doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text
35218806	5	0	theme	scanning	1019:1026	arg1	SEM					1049:1051	SEM	1049:1051	SEM	1049:1051	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	0	theme	scanning	1019:1026	arg1	microscopy					1037:1046	scanning electron microscopy	1019:1046	scanning electron microscopy (SEM)	1019:1052	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	3	1	theme	cancer	479:484	arg1	treatment					461:469	the treatment	457:469	the treatment of skin cancer	457:484	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	5	2	theme	electron	1028:1035	arg1	SEM					1049:1051	SEM	1049:1051	SEM	1049:1051	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	2	theme	electron	1028:1035	arg1	microscopy					1037:1046	scanning electron microscopy	1019:1046	scanning electron microscopy (SEM)	1019:1052	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	2	3	theme	side	346:349	arg1	effects					351:357	severe side effects	339:357	severe side effects	339:357	Despite its proven efficacy in treating malignancies, its systemic administration is limited due to severe side effects.
35218806	8	4	theme	delivery	1325:1332	arg1	platform					1334:1341	a promising delivery platform	1313:1341	a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity	1313:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	8	4	theme	delivery	1325:1332	arg1	Mic-Hyd					1296:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	0	5	theme	deoxycholic	100:110	arg1	acid					112:115	deoxycholic acid	100:115	deoxycholic acid	100:115	Controlled release of 5-fluorouracil to melanoma cells using a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan.
35218806	7	6	theme	anticancer	1219:1228	arg1	activity					1230:1237	enhanced anticancer activity	1210:1237	enhanced anticancer activity than 5-FU against the growth of melanoma cells	1210:1284	The final formulation was demonstrated to have enhanced anticancer activity than 5-FU against the growth of melanoma cells.
35218806	8	7	theme	@	1295:1295	arg1	platform					1334:1341	a promising delivery platform	1313:1341	a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity	1313:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	8	7	theme	@	1295:1295	arg1	Mic-Hyd					1296:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	5	8	theme	Mic/Hyd	861:867	arg1	system					869:874	the Mic/Hyd system	857:874	the Mic/Hyd system	857:874	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	8	9	theme	systemic	1407:1414	arg1	toxicity					1416:1423	systemic toxicity	1407:1423	systemic toxicity	1407:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	5	10	theme	Fourier-transform	895:911	arg1	FT-IR					936:940	FT-IR	936:940	FT-IR	936:940	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	10	theme	Fourier-transform	895:911	arg1	spectroscopy					922:933	Fourier-transform infrared spectroscopy	895:933	Fourier-transform infrared spectroscopy (FT-IR)	895:941	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	6	11	theme	Hyd	1158:1160	arg1	properties					1140:1149	pH-dependent properties	1127:1149	pH-dependent properties of the Hyd	1127:1160	Drug release studies showed pH-dependent properties of the Hyd.
35218806	4	12	theme	CMC	707:709	arg1	hydrogel					697:704	carboxymethyl chitosan hydrogel	674:704	carboxymethyl chitosan hydrogel (CMC Hyd)	674:714	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	12	theme	CMC	707:709	arg1	Hyd					711:713	CMC Hyd	707:713	CMC Hyd	707:713	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	8	13	from	efficacy	1357:1364	arg1	management					1373:1382	the management	1369:1382	the management of skin cancer without systemic toxicity	1369:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	4	14	theme	systemic	776:783	arg1	effects					790:796	its systemic side effects	772:796	its systemic side effects	772:796	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	2	15	theme	treating	270:277	arg1	malignancies					279:290	treating malignancies	270:290	treating malignancies	270:290	Despite its proven efficacy in treating malignancies, its systemic administration is limited due to severe side effects.
35218806	6	16	theme	pH-dependent	1127:1138	arg1	properties					1140:1149	pH-dependent properties	1127:1149	pH-dependent properties of the Hyd	1127:1160	Drug release studies showed pH-dependent properties of the Hyd.
35218806	3	17	theme	poor	500:503	arg1	permeability					505:516	the poor permeability	496:516	the poor permeability of 5-FU through the skin	496:541	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	3	17	theme	poor	500:503	arg1	challenge					554:562	a challenge	552:562	a challenge	552:562	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	5	18	theme	infrared	913:920	arg1	FT-IR					936:940	FT-IR	936:940	FT-IR	936:940	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	18	theme	infrared	913:920	arg1	spectroscopy					922:933	Fourier-transform infrared spectroscopy	895:933	Fourier-transform infrared spectroscopy (FT-IR)	895:941	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	1	19	theme	antimetabolite	173:186	arg1	drug					188:191	an antimetabolite drug	170:191	an antimetabolite drug widely used for the treatment of skin cancer	170:236	5-Fluorouracil (5-FU) is an antimetabolite drug widely used for the treatment of skin cancer.
35218806	1	19	theme	antimetabolite	173:186	arg1	5-Fluorouracil					145:158	5-Fluorouracil	145:158	5-Fluorouracil (5-FU)	145:165	5-Fluorouracil (5-FU) is an antimetabolite drug widely used for the treatment of skin cancer.
35218806	4	20	theme	chitosan	688:695	arg1	hydrogel					697:704	carboxymethyl chitosan hydrogel	674:704	carboxymethyl chitosan hydrogel (CMC Hyd)	674:714	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	20	theme	chitosan	688:695	arg1	Hyd					711:713	CMC Hyd	707:713	CMC Hyd	707:713	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	8	21	theme	cancer	1392:1397	arg1	management					1373:1382	the management	1369:1382	the management of skin cancer without systemic toxicity	1369:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	4	22	theme	side	785:788	arg1	effects					790:796	its systemic side effects	772:796	its systemic side effects	772:796	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	23	theme	carboxymethyl	674:686	arg1	hydrogel					697:704	carboxymethyl chitosan hydrogel	674:704	carboxymethyl chitosan hydrogel (CMC Hyd)	674:714	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	23	theme	carboxymethyl	674:686	arg1	Hyd					711:713	CMC Hyd	707:713	CMC Hyd	707:713	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	7	24	theme	enhanced	1210:1217	arg1	activity					1230:1237	enhanced anticancer activity	1210:1237	enhanced anticancer activity than 5-FU against the growth of melanoma cells	1210:1284	The final formulation was demonstrated to have enhanced anticancer activity than 5-FU against the growth of melanoma cells.
35218806	0	25	theme	5-fluorouracil	22:35	arg1	release					11:17	release	11:17	release of 5-fluorouracil to melanoma cells using a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan	11:142	Controlled release of 5-fluorouracil to melanoma cells using a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan.
35218806	5	26	theme	transmission	1059:1070	arg1	TEM					1093:1095	TEM	1093:1095	TEM	1093:1095	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	26	theme	transmission	1059:1070	arg1	microscopy					1081:1090	transmission electron microscopy	1059:1090	transmission electron microscopy (TEM)	1059:1096	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	8	27	theme	5-FU	1291:1294	arg1	platform					1334:1341	a promising delivery platform	1313:1341	a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity	1313:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	8	27	theme	5-FU	1291:1294	arg1	Mic-Hyd					1296:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	3	28	theme	5-FU	521:524	arg1	permeability					505:516	the poor permeability	496:516	the poor permeability of 5-FU through the skin	496:541	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	3	28	theme	5-FU	521:524	arg1	challenge					554:562	a challenge	552:562	a challenge	552:562	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	7	29	theme	melanoma	1271:1278	arg1	cells					1280:1284	melanoma cells	1271:1284	melanoma cells	1271:1284	The final formulation was demonstrated to have enhanced anticancer activity than 5-FU against the growth of melanoma cells.
35218806	5	30	theme	system	869:874	arg1	properties					843:852	The properties	839:852	The properties of the Mic/Hyd system	839:874	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	31	theme	electron	1072:1079	arg1	TEM					1093:1095	TEM	1093:1095	TEM	1093:1095	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	31	theme	electron	1072:1079	arg1	microscopy					1081:1090	transmission electron microscopy	1059:1090	transmission electron microscopy (TEM)	1059:1096	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	0	32	theme	carboxymethyl	121:133	arg1	chitosan					135:142	carboxymethyl chitosan	121:142	carboxymethyl chitosan	121:142	Controlled release of 5-fluorouracil to melanoma cells using a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan.
35218806	5	33	theme	sizer	981:985	arg1	zeta					976:979	zeta sizer	976:985	zeta sizer	976:985	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	2	34	from	efficacy	258:265	arg1	malignancies					279:290	treating malignancies	270:290	treating malignancies	270:290	Despite its proven efficacy in treating malignancies, its systemic administration is limited due to severe side effects.
35218806	0	35	theme	melanoma	40:47	arg1	cells					49:53	melanoma cells	40:53	melanoma cells using a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan	40:142	Controlled release of 5-fluorouracil to melanoma cells using a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan.
35218806	4	36	theme	DCA	661:663	arg1	micelle					652:658	deoxycholic acid micelle	635:658	deoxycholic acid micelle (DCA Mic)	635:668	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	36	theme	DCA	661:663	arg1	Mic					665:667	DCA Mic	661:667	DCA Mic	661:667	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	8	37	theme	promising	1315:1323	arg1	platform					1334:1341	a promising delivery platform	1313:1341	a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity	1313:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	8	37	theme	promising	1315:1323	arg1	Mic-Hyd					1296:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd	1287:1302	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	5	38	theme	dynamic	944:950	arg1	DLS					970:972	DLS	970:972	DLS	970:972	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	38	theme	dynamic	944:950	arg1	scattering					958:967	dynamic light scattering	944:967	dynamic light scattering (DLS)	944:973	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	4	39	theme	micellar	601:608	arg1	system					619:624	a pH-responsive micellar hydrogel system	585:624	a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd)	585:714	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	3	40	theme	5-FU	403:406	arg1	delivery					391:398	topical delivery	383:398	topical delivery of 5-FU	383:406	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	3	40	theme	5-FU	403:406	arg1	approach					444:451	an alternative approach	429:451	an alternative approach for the treatment of skin cancer	429:484	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	5	41	theme	atomic	988:993	arg1	AFM					1013:1015	AFM	1013:1015	AFM	1013:1015	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	41	theme	atomic	988:993	arg1	microscopy					1001:1010	atomic force microscopy	988:1010	atomic force microscopy (AFM)	988:1016	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	4	42	theme	skin	749:752	arg1	cancer					754:759	skin cancer	749:759	skin cancer	749:759	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	3	43	theme	alternative	432:442	arg1	delivery					391:398	topical delivery	383:398	topical delivery of 5-FU	383:406	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	3	43	theme	alternative	432:442	arg1	approach					444:451	an alternative approach	429:451	an alternative approach for the treatment of skin cancer	429:484	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	3	44	theme	topical	383:389	arg1	delivery					391:398	topical delivery	383:398	topical delivery of 5-FU	383:406	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	3	44	theme	topical	383:389	arg1	approach					444:451	an alternative approach	429:451	an alternative approach for the treatment of skin cancer	429:484	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	5	45	theme	force	995:999	arg1	AFM					1013:1015	AFM	1013:1015	AFM	1013:1015	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	45	theme	force	995:999	arg1	microscopy					1001:1010	atomic force microscopy	988:1010	atomic force microscopy (AFM)	988:1016	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	6	46	theme	release	1104:1110	arg1	studies					1112:1118	Drug release studies	1099:1118	Drug release studies	1099:1118	Drug release studies showed pH-dependent properties of the Hyd.
35218806	2	47	theme	severe	339:344	arg1	effects					351:357	severe side effects	339:357	severe side effects	339:357	Despite its proven efficacy in treating malignancies, its systemic administration is limited due to severe side effects.
35218806	8	48	theme	skin	1387:1390	arg1	cancer					1392:1397	skin cancer	1387:1397	skin cancer	1387:1397	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	6	49	theme	Drug	1099:1102	arg1	studies					1112:1118	Drug release studies	1099:1118	Drug release studies	1099:1118	Drug release studies showed pH-dependent properties of the Hyd.
35218806	3	50	theme	skin	474:477	arg1	cancer					479:484	skin cancer	474:484	skin cancer	474:484	To address this issue, topical delivery of 5-FU has been proposed as an alternative approach for the treatment of skin cancer, however, the poor permeability of 5-FU through the skin is still a challenge.
35218806	7	51	contain	have	1205:1208	arg2	activity					1230:1237	enhanced anticancer activity	1210:1237	enhanced anticancer activity than 5-FU against the growth of melanoma cells	1210:1284	The final formulation was demonstrated to have enhanced anticancer activity than 5-FU against the growth of melanoma cells.
35218806	7	51	contain	have	1205:1208	arg1	formulation					1173:1183	The final formulation	1163:1183	The final formulation	1163:1183	The final formulation was demonstrated to have enhanced anticancer activity than 5-FU against the growth of melanoma cells.
35218806	4	52	theme	5-FU	727:730	arg1	efficacy					732:739	5-FU efficacy	727:739	5-FU efficacy against skin cancer	727:759	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	5	53	theme	light	952:956	arg1	DLS					970:972	DLS	970:972	DLS	970:972	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	5	53	theme	light	952:956	arg1	scattering					958:967	dynamic light scattering	944:967	dynamic light scattering (DLS)	944:973	The properties of the Mic/Hyd system were determined by Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), zeta sizer, atomic force microscopy (AFM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM).
35218806	2	54	theme	systemic	297:304	arg1	administration					306:319	its systemic administration	293:319	its systemic administration	293:319	Despite its proven efficacy in treating malignancies, its systemic administration is limited due to severe side effects.
35218806	2	55	theme	proven	251:256	arg1	efficacy					258:265	its proven efficacy	247:265	its proven efficacy in treating malignancies	247:290	Despite its proven efficacy in treating malignancies, its systemic administration is limited due to severe side effects.
35218806	8	56	theme	enhanced	1348:1355	arg1	efficacy					1357:1364	enhanced efficacy	1348:1364	enhanced efficacy in the management of skin cancer without systemic toxicity	1348:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	1	57	theme	skin	226:229	arg1	cancer					231:236	skin cancer	226:236	skin cancer	226:236	5-Fluorouracil (5-FU) is an antimetabolite drug widely used for the treatment of skin cancer.
35218806	0	58	theme	hydrogel/micelle	63:78	arg1	composites					80:89	a hydrogel/micelle composites	61:89	a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan	61:142	Controlled release of 5-fluorouracil to melanoma cells using a hydrogel/micelle composites based on deoxycholic acid and carboxymethyl chitosan.
35218806	4	59	theme	pH-responsive	587:599	arg1	system					619:624	a pH-responsive micellar hydrogel system	585:624	a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd)	585:714	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	8	60	with	platform	1334:1341	arg1	efficacy					1357:1364	enhanced efficacy	1348:1364	enhanced efficacy in the management of skin cancer without systemic toxicity	1348:1423	The 5-FU@Mic-Hyd could be a promising delivery platform with enhanced efficacy in the management of skin cancer without systemic toxicity.
35218806	4	61	theme	acid	647:650	arg1	micelle					652:658	deoxycholic acid micelle	635:658	deoxycholic acid micelle (DCA Mic)	635:668	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	61	theme	acid	647:650	arg1	Mic					665:667	DCA Mic	661:667	DCA Mic	661:667	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	62	theme	hydrogel	610:617	arg1	system					619:624	a pH-responsive micellar hydrogel system	585:624	a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd)	585:714	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	1	63	theme	cancer	231:236	arg1	treatment					213:221	the treatment	209:221	the treatment of skin cancer	209:236	5-Fluorouracil (5-FU) is an antimetabolite drug widely used for the treatment of skin cancer.
35218806	7	64	theme	final	1167:1171	arg1	formulation					1173:1183	The final formulation	1163:1183	The final formulation	1163:1183	The final formulation was demonstrated to have enhanced anticancer activity than 5-FU against the growth of melanoma cells.
35218806	4	65	theme	deoxycholic	635:645	arg1	micelle					652:658	deoxycholic acid micelle	635:658	deoxycholic acid micelle (DCA Mic)	635:668	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	4	65	theme	deoxycholic	635:645	arg1	Mic					665:667	DCA Mic	661:667	DCA Mic	661:667	Here, we introduced a pH-responsive micellar hydrogel system based on deoxycholic acid micelle (DCA Mic) and carboxymethyl chitosan hydrogel (CMC Hyd) to enhance 5-FU efficacy against skin cancer and reduce its systemic side effects by improving its delivery into the skin.
35218806	7	66	theme	cells	1280:1284	arg1	growth					1261:1266	the growth	1257:1266	the growth of melanoma cells	1257:1284	The final formulation was demonstrated to have enhanced anticancer activity than 5-FU against the growth of melanoma cells.
36552848	11	0	theme	depth	1967:1971	arg1	ratios					1973:1978	lesion depth ratios	1960:1978	lesion depth ratios (44%)	1960:1984	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	0	theme	depth	1967:1971	arg1	%					1983:1983	44%	1981:1983	44%	1981:1983	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	12	1	theme	damage	2117:2122	arg1	evaluation					2086:2095	histopathologic evaluation	2070:2095	histopathologic evaluation of subchondral bone damage	2070:2122	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	6	2	theme	intra-articularly	1037:1053	arg1	doses					1031:1035	two doses intra-articularly	1027:1053	two doses intra-articularly (IA)	1027:1058	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	6	2	theme	intra-articularly	1037:1053	arg1	IA					1056:1057	IA	1056:1057	IA	1056:1057	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	10	3	theme	1D	1778:1779	arg1	dose					1756:1759	the high dose	1747:1759	the high dose (i.e., 24 µg)	1747:1773	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	10	3	theme	1D	1778:1779	arg1	µg					1771:1772	24 µg	1768:1772	24 µg	1768:1772	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	2	4	theme	HU308	323:327	arg1	effects					312:318	The effects	308:318	The effects of HU308	308:327	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	2	4	theme	HU308	323:327	arg1	related					342:348	related	342:348	related	342:348	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	13	5	theme	increased	2304:2312	arg1	size					2325:2328	increased osteophyte size	2304:2328	increased osteophyte size	2304:2328	Contrary to the anabolic effect of FGF18, synovial inflammation was observed and was accompanied by increased osteophyte size.
36552848	9	6	theme	Co-administering	1554:1569	arg1	antagonists					1577:1587	Co-administering a CB2 antagonists	1554:1587	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model	1554:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	7	7	theme	International	1345:1357	arg1	guidelines					1367:1376	the Osteoarthritis Research Society International (OARSI) guidelines	1309:1376	the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT	1309:1418	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	9	8	theme	CB2	1573:1575	arg1	antagonists					1577:1587	Co-administering a CB2 antagonists	1554:1587	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model	1554:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	7	9	theme	Research	1328:1335	arg1	guidelines					1367:1376	the Osteoarthritis Research Society International (OARSI) guidelines	1309:1376	the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT	1309:1418	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	10	10	theme	IA	1798:1799	arg1	route					1801:1805	IA route	1798:1805	IA route	1798:1805	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	1	11	theme	receptor	180:187	arg1	agonist					197:203	the cannabinoid receptor 2 (CB2) agonist HU308	164:209	the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice	164:305	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	6	12	theme	tear	1097:1100	arg1	model					1120:1124	a post-traumatic medial meniscal tear (MMT, Lewis rats) model	1064:1124	a post-traumatic medial meniscal tear (MMT, Lewis rats) model	1064:1124	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	9	13	theme	inflammatory	1613:1624	arg1	model					1631:1635	a paw inflammatory pain model	1607:1635	a paw inflammatory pain model	1607:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	6	14	dep	tear	1097:1100	arg1	MMT					1103:1105	MMT	1103:1105	MMT	1103:1105	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	6	14	dep	tear	1097:1100	arg1	rats					1114:1117	Lewis rats	1108:1117	Lewis rats	1108:1117	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	4	15	theme	Gi-protein	887:896	arg1	pathway					912:918	the Gi-protein receptor cAMP pathway	883:918	the Gi-protein receptor cAMP pathway	883:918	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	6	16	theme	medial	1081:1086	arg1	tear					1097:1100	medial meniscal tear	1081:1100	a post-traumatic medial meniscal tear (MMT, Lewis rats) model	1064:1124	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	10	17	theme	reduced	1818:1824	arg1	damage					1836:1841	reduced cartilage damage	1818:1841	reduced cartilage damage	1818:1841	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	8	18	from	effects	1511:1517	arg1	lines					1527:1531	cell lines	1522:1531	cell lines	1522:1531	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	8	18	from	effects	1511:1517	arg1	chondrocytes					1540:1551	chondrocytes	1540:1551	chondrocytes	1540:1551	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	11	19	theme	30	1886:1887	arg1	%					1888:1888	%	1888:1888	%	1888:1888	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	1	20	theme	previous	128:135	arg1	report					137:142	a previous report	126:142	a previous report	126:142	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	4	21	theme	cAMP	907:910	arg1	pathway					912:918	the Gi-protein receptor cAMP pathway	883:918	the Gi-protein receptor cAMP pathway	883:918	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	3	22	theme	novel	581:585	arg1	agonists					612:619	the novel cannabinoid-fenchone CB2 agonists	577:619	the novel cannabinoid-fenchone CB2 agonists (1B, 1D)	577:628	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	9	23	theme	paw	1609:1611	arg1	model					1631:1635	a paw inflammatory pain model	1607:1635	a paw inflammatory pain model	1607:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	8	24	contain	possess	1489:1495	arg1	1D					1486:1487	1D	1486:1487	1D	1486:1487	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	8	24	contain	possess	1489:1495	arg1	1B					1479:1480	1B	1479:1480	1B	1479:1480	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	8	24	contain	possess	1489:1495	arg2	effects					1511:1517	CB2 agonistic effects	1497:1517	CB2 agonistic effects in cell lines and in chondrocytes	1497:1551	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	3	25	used	utilized	568:575	arg2	we					565:566	we	565:566	we	565:566	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	6	26	theme	growth	1206:1211	arg1	factor					1213:1218	fibroblast growth factor 18	1195:1221	fibroblast growth factor 18 (FGF18) administration	1195:1244	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	6	26	theme	growth	1206:1211	arg1	FGF18					1224:1228	FGF18	1224:1228	FGF18	1224:1228	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	4	27	dep	METHODS	718:724	arg1	assessed					740:747	assessed	740:747	assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway	740:918	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	14	28	theme	joint	2490:2494	arg1	structure					2496:2504	the joint structure	2486:2504	the joint structure	2486:2504	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	9	29	theme	pain	1626:1629	arg1	model					1631:1635	a paw inflammatory pain model	1607:1635	a paw inflammatory pain model	1607:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	14	30	dep	CONCLUSION	2331:2340	arg1	supports					2384:2391	supports	2384:2391	supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure	2384:2504	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	6	31	theme	Lewis	1108:1112	arg1	MMT					1103:1105	MMT	1103:1105	MMT	1103:1105	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	6	31	theme	Lewis	1108:1112	arg1	rats					1114:1117	Lewis rats	1108:1117	Lewis rats	1108:1117	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	1	32	dep	BACKGROUND	112:121	arg1	identified					153:162	identified	153:162	have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice	148:305	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	0	33	theme	Post-Traumatic	67:80	arg1	Osteoarthritis					82:95	Post-Traumatic Osteoarthritis	67:95	Post-Traumatic Osteoarthritis in Lewis Rats	67:109	A Fenchone Derivative Effectively Abrogates Joint Damage Following Post-Traumatic Osteoarthritis in Lewis Rats.
36552848	3	34	theme	CB2	608:610	arg1	agonists					612:619	the novel cannabinoid-fenchone CB2 agonists	577:619	the novel cannabinoid-fenchone CB2 agonists (1B, 1D)	577:628	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	14	35	theme	deleterious	2463:2473	arg1	effects					2475:2481	any deleterious effects	2459:2481	any deleterious effects on the joint structure	2459:2504	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	9	36	theme	anti-swelling	1658:1670	arg1	effect					1672:1677	1D-related anti-swelling effect	1647:1677	1D-related anti-swelling effect	1647:1677	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	11	37	theme	tibial	1941:1946	arg1	degeneration					1915:1926	cartilage degeneration	1905:1926	cartilage degeneration (zonal/total tibial scores)	1905:1954	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	37	theme	tibial	1941:1946	arg1	scores					1948:1953	zonal/total tibial scores	1929:1953	zonal/total tibial scores	1929:1953	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	38	theme	lesion	1960:1965	arg1	ratios					1973:1978	lesion depth ratios	1960:1978	lesion depth ratios (44%)	1960:1984	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	38	theme	lesion	1960:1965	arg1	%					1983:1983	44%	1981:1983	44%	1981:1983	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	12	39	theme	histopathologic	2070:2084	arg1	evaluation					2086:2095	histopathologic evaluation	2070:2095	histopathologic evaluation of subchondral bone damage	2070:2122	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	9	40	from	1D	1601:1602	arg1	model					1631:1635	a paw inflammatory pain model	1607:1635	a paw inflammatory pain model	1607:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	12	41	theme	subchondral	2100:2110	arg1	damage					2117:2122	subchondral bone damage	2100:2122	subchondral bone damage	2100:2122	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	13	42	theme	osteophyte	2314:2323	arg1	size					2325:2328	increased osteophyte size	2304:2328	increased osteophyte size	2304:2328	Contrary to the anabolic effect of FGF18, synovial inflammation was observed and was accompanied by increased osteophyte size.
36552848	9	43	theme	analgesic	1708:1716	arg1	effects					1718:1724	its analgesic effects	1704:1724	its analgesic effects	1704:1724	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	8	44	theme	protein	1435:1441	arg1	assays					1452:1457	The G protein receptor assays	1429:1457	The G protein receptor assays	1429:1457	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	14	45	theme	1D	2439:2440	arg1	compound					2442:2449	IA-administered 1D compound	2423:2449	IA-administered 1D compound	2423:2449	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	13	46	theme	synovial	2246:2253	arg1	inflammation					2255:2266	synovial inflammation	2246:2266	synovial inflammation	2246:2266	Contrary to the anabolic effect of FGF18, synovial inflammation was observed and was accompanied by increased osteophyte size.
36552848	10	47	theme	MMT	1736:1738	arg1	model					1740:1744	an MMT model	1733:1744	an MMT model	1733:1744	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	13	48	theme	anabolic	2220:2227	arg1	effect					2229:2234	the anabolic effect	2216:2234	the anabolic effect of FGF18	2216:2243	Contrary to the anabolic effect of FGF18, synovial inflammation was observed and was accompanied by increased osteophyte size.
36552848	14	49	theme	IA-administered	2423:2437	arg1	compound					2442:2449	IA-administered 1D compound	2423:2449	IA-administered 1D compound	2423:2449	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	2	50	theme	CREB/SOX9	440:448	arg1	axis					450:453	the CREB/SOX9 axis	436:453	the CREB/SOX9 axis	436:453	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	4	51	theme	Gs-protein	780:789	arg1	assay					813:817	a Gs-protein receptor cAMP potency assay	778:817	a Gs-protein receptor cAMP potency assay	778:817	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	2	52	theme	compound	374:381	arg1	capacity					357:364	the capacity	353:364	the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment	353:556	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	5	53	theme	inflammatory	973:984	arg1	model					991:995	a zymosan inflammatory pain model	963:995	a zymosan inflammatory pain model	963:995	Based on EC50 values, 1D was selected for a zymosan inflammatory pain model.
36552848	11	54	theme	FGF18	2005:2009	arg1	control					2020:2026	the FGF18 positive control	2001:2026	the FGF18 positive control	2001:2026	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	4	55	theme	cAMP	800:803	arg1	assay					813:817	a Gs-protein receptor cAMP potency assay	778:817	a Gs-protein receptor cAMP potency assay	778:817	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	13	56	theme	FGF18	2239:2243	arg1	effect					2229:2234	the anabolic effect	2216:2234	the anabolic effect of FGF18	2216:2243	Contrary to the anabolic effect of FGF18, synovial inflammation was observed and was accompanied by increased osteophyte size.
36552848	11	57	theme	comparable	1987:1996	arg1	improvement					1890:1900	a 30% improvement	1884:1900	a 30% improvement	1884:1900	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	8	58	theme	CB2	1497:1499	arg1	effects					1511:1517	CB2 agonistic effects	1497:1517	CB2 agonistic effects in cell lines and in chondrocytes	1497:1551	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	14	59	from	effects	2475:2481	arg1	structure					2496:2504	the joint structure	2486:2504	the joint structure	2486:2504	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	14	60	theme	structural	2346:2355	arg1	analysis					2375:2382	The structural histopathological analysis	2342:2382	The structural histopathological analysis	2342:2382	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	3	61	theme	anti-inflammatory	672:688	arg1	effects					690:696	anti-inflammatory effects	672:696	anti-inflammatory effects	672:696	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	8	62	dep	RESULTS	1421:1427	arg1	confirmed					1459:1467	confirmed	1459:1467	confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes	1459:1551	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	7	63	theme	rat	1382:1384	arg1	models					1386:1391	rat models	1382:1391	rat models following 28 days post-MMT	1382:1418	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	8	64	theme	cell	1522:1525	arg1	lines					1527:1531	cell lines	1522:1531	cell lines	1522:1531	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	6	65	theme	positive	1164:1171	arg1	control					1173:1179	a positive control	1162:1179	a positive control consisting of fibroblast growth factor 18 (FGF18) administration	1162:1244	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	11	66	theme	zonal/total	1929:1939	arg1	degeneration					1915:1926	cartilage degeneration	1905:1926	cartilage degeneration (zonal/total tibial scores)	1905:1954	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	66	theme	zonal/total	1929:1939	arg1	scores					1948:1953	zonal/total tibial scores	1929:1953	zonal/total tibial scores	1929:1953	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	67	theme	1D	1871:1872	arg1	dose					1863:1866	this dose	1858:1866	this dose of 1D	1858:1872	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	9	68	theme	a	1571:1571	arg1	antagonists					1577:1587	Co-administering a CB2 antagonists	1554:1587	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model	1554:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	3	69	theme	zymosan	703:709	arg1	model					711:715	a zymosan model	701:715	a zymosan model	701:715	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	1	70	from	osteoarthritis	279:292	arg1	mice					302:305	mice	302:305	mice	302:305	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	1	71	theme	cannabinoid	168:178	arg1	agonist					197:203	the cannabinoid receptor 2 (CB2) agonist HU308	164:209	the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice	164:305	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	11	72	theme	positive	2011:2018	arg1	control					2020:2026	the FGF18 positive control	2001:2026	the FGF18 positive control	2001:2026	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	7	73	theme	OARSI	1360:1364	arg1	guidelines					1367:1376	the Osteoarthritis Research Society International (OARSI) guidelines	1309:1376	the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT	1309:1418	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	4	74	theme	potency	805:811	arg1	assay					813:817	a Gs-protein receptor cAMP potency assay	778:817	a Gs-protein receptor cAMP potency assay	778:817	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	7	75	theme	Society	1337:1343	arg1	guidelines					1367:1376	the Osteoarthritis Research Society International (OARSI) guidelines	1309:1376	the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT	1309:1418	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	11	76	theme	%	1888:1888	arg1	improvement					1890:1900	a 30% improvement	1884:1900	a 30% improvement	1884:1900	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	9	77	theme	25	1592:1593	arg1	mg/kg					1595:1599	mg/kg	1595:1599	mg/kg	1595:1599	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	7	78	theme	Osteoarthritis	1313:1326	arg1	guidelines					1367:1376	the Osteoarthritis Research Society International (OARSI) guidelines	1309:1376	the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT	1309:1418	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	10	79	theme	cartilage	1826:1834	arg1	damage					1836:1841	reduced cartilage damage	1818:1841	reduced cartilage damage	1818:1841	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	1	80	theme	CB2	192:194	arg1	agonist					197:203	the cannabinoid receptor 2 (CB2) agonist HU308	164:209	the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice	164:305	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	6	81	theme	meniscal	1088:1095	arg1	tear					1097:1100	medial meniscal tear	1081:1100	a post-traumatic medial meniscal tear (MMT, Lewis rats) model	1064:1124	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	2	82	theme	cartilage	528:536	arg1	hallmarks					505:513	pro-survival and pro-proliferative hallmarks	470:513	pro-survival and pro-proliferative hallmarks of articular cartilage	470:536	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	6	83	theme	post-traumatic	1066:1079	arg1	model					1120:1124	a post-traumatic medial meniscal tear (MMT, Lewis rats) model	1064:1124	a post-traumatic medial meniscal tear (MMT, Lewis rats) model	1064:1124	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	3	84	dep	agonists	612:619	arg1	1D					626:627	1D	626:627	1D	626:627	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	3	84	dep	agonists	612:619	arg1	1B					622:623	1B	622:623	1B	622:623	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	4	85	theme	receptor	898:905	arg1	pathway					912:918	the Gi-protein receptor cAMP pathway	883:918	the Gi-protein receptor cAMP pathway	883:918	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	0	86	from	Osteoarthritis	82:95	arg1	Rats					106:109	Lewis Rats	100:109	Lewis Rats	100:109	A Fenchone Derivative Effectively Abrogates Joint Damage Following Post-Traumatic Osteoarthritis in Lewis Rats.
36552848	6	87	theme	factor	1213:1218	arg1	administration					1231:1244	fibroblast growth factor 18 (FGF18) administration	1195:1244	fibroblast growth factor 18 (FGF18) administration	1195:1244	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	4	88	theme	antagonistic	849:860	arg1	effects					862:868	antagonistic effects	849:868	antagonistic effects dependent on the Gi-protein receptor cAMP pathway	849:918	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	6	89	theme	fibroblast	1195:1204	arg1	factor					1213:1218	fibroblast growth factor 18	1195:1221	fibroblast growth factor 18 (FGF18) administration	1195:1244	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	6	89	theme	fibroblast	1195:1204	arg1	FGF18					1224:1228	FGF18	1224:1228	FGF18	1224:1228	Next, 1D was administered in two doses intra-articularly (IA), in a post-traumatic medial meniscal tear (MMT, Lewis rats) model, and compared to sham, vehicle, and a positive control consisting of fibroblast growth factor 18 (FGF18) administration.
36552848	4	90	theme	dependent	870:878	arg1	effects					862:868	antagonistic effects	849:868	antagonistic effects dependent on the Gi-protein receptor cAMP pathway	849:918	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	12	91	theme	load-bearing	2170:2181	arg1	ability					2183:2189	the load-bearing ability	2166:2189	the load-bearing ability of the rats	2166:2201	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	7	92	theme	histopathological	1251:1267	arg1	assessment					1269:1278	The histopathological assessment	1247:1278	The histopathological assessment	1247:1278	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	3	93	theme	cannabinoid-fenchone	587:606	arg1	agonists					612:619	the novel cannabinoid-fenchone CB2 agonists	577:619	the novel cannabinoid-fenchone CB2 agonists (1B, 1D)	577:628	Here, we utilized the novel cannabinoid-fenchone CB2 agonists (1B, 1D), which were previously reported to render anti-inflammatory effects in a zymosan model.
36552848	9	94	theme	1D-related	1647:1656	arg1	effect					1672:1677	1D-related anti-swelling effect	1647:1677	1D-related anti-swelling effect	1647:1677	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	0	95	theme	Lewis	100:104	arg1	Rats					106:109	Lewis Rats	100:109	Lewis Rats	100:109	A Fenchone Derivative Effectively Abrogates Joint Damage Following Post-Traumatic Osteoarthritis in Lewis Rats.
36552848	12	96	theme	bone	2112:2115	arg1	damage					2117:2122	subchondral bone damage	2100:2122	subchondral bone damage	2100:2122	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	1	97	from	age	256:258	arg1	mice					302:305	mice	302:305	mice	302:305	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	12	98	theme	1D	2145:2146	arg1	treatment					2148:2156	1D treatment	2145:2156	1D treatment	2145:2156	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	5	99	theme	EC50	930:933	arg1	values					935:940	EC50 values	930:940	EC50 values	930:940	Based on EC50 values, 1D was selected for a zymosan inflammatory pain model.
36552848	14	100	theme	compound	2442:2449	arg1	effect					2413:2418	a disease-modifying effect	2393:2418	a disease-modifying effect of IA-administered 1D compound	2393:2449	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	2	101	theme	pro-proliferative	487:503	arg1	hallmarks					505:513	pro-survival and pro-proliferative hallmarks	470:513	pro-survival and pro-proliferative hallmarks of articular cartilage	470:536	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	8	102	theme	G	1433:1433	arg1	assays					1452:1457	The G protein receptor assays	1429:1457	The G protein receptor assays	1429:1457	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	2	103	theme	pro-survival	470:481	arg1	hallmarks					505:513	pro-survival and pro-proliferative hallmarks	470:513	pro-survival and pro-proliferative hallmarks of articular cartilage	470:536	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	8	104	theme	receptor	1443:1450	arg1	assays					1452:1457	The G protein receptor assays	1429:1457	The G protein receptor assays	1429:1457	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	0	105	theme	Fenchone	2:9	arg1	Derivative					11:20	A Fenchone Derivative	0:20	A Fenchone Derivative	0:20	A Fenchone Derivative Effectively Abrogates Joint Damage Following Post-Traumatic Osteoarthritis in Lewis Rats.
36552848	2	106	theme	articular	518:526	arg1	cartilage					528:536	articular cartilage	518:536	articular cartilage	518:536	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	14	107	theme	histopathological	2357:2373	arg1	analysis					2375:2382	The structural histopathological analysis	2342:2382	The structural histopathological analysis	2342:2382	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	12	108	theme	Synovitis	2029:2037	arg1	scores					2039:2044	Synovitis scores	2029:2044	Synovitis scores	2029:2044	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	1	109	theme	trauma-induced	264:277	arg1	osteoarthritis					279:292	trauma-induced osteoarthritis	264:292	trauma-induced osteoarthritis (OA)	264:297	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	1	109	theme	trauma-induced	264:277	arg1	OA					295:296	OA	295:296	OA	295:296	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
36552848	2	110	theme	cartilage	393:401	arg1	anabolism					403:411	cartilage anabolism	393:411	cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment	393:556	The effects of HU308 were largely related to the capacity of this compound to induce cartilage anabolism which was dependent on the CREB/SOX9 axis, and exhibited pro-survival and pro-proliferative hallmarks of articular cartilage following treatment.
36552848	0	111	theme	Joint	44:48	arg1	Damage					50:55	Joint Damage	44:55	Joint Damage	44:55	A Fenchone Derivative Effectively Abrogates Joint Damage Following Post-Traumatic Osteoarthritis in Lewis Rats.
36552848	5	112	theme	zymosan	965:971	arg1	model					991:995	a zymosan inflammatory pain model	963:995	a zymosan inflammatory pain model	963:995	Based on EC50 values, 1D was selected for a zymosan inflammatory pain model.
36552848	10	113	theme	high	1751:1754	arg1	dose					1756:1759	the high dose	1747:1759	the high dose (i.e., 24 µg)	1747:1773	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	10	113	theme	high	1751:1754	arg1	µg					1771:1772	24 µg	1768:1772	24 µg	1768:1772	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	14	114	theme	disease-modifying	2395:2411	arg1	effect					2413:2418	a disease-modifying effect	2393:2418	a disease-modifying effect of IA-administered 1D compound	2393:2449	CONCLUSION The structural histopathological analysis supports a disease-modifying effect of IA-administered 1D compound without any deleterious effects on the joint structure.
36552848	5	115	theme	pain	986:989	arg1	model					991:995	a zymosan inflammatory pain model	963:995	a zymosan inflammatory pain model	963:995	Based on EC50 values, 1D was selected for a zymosan inflammatory pain model.
36552848	11	116	theme	cartilage	1905:1913	arg1	degeneration					1915:1926	cartilage degeneration	1905:1926	cartilage degeneration (zonal/total tibial scores)	1905:1954	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	116	theme	cartilage	1905:1913	arg1	scores					1948:1953	zonal/total tibial scores	1929:1953	zonal/total tibial scores	1929:1953	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	4	117	theme	receptor	791:798	arg1	assay					813:817	a Gs-protein receptor cAMP potency assay	778:817	a Gs-protein receptor cAMP potency assay	778:817	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	12	118	theme	rats	2198:2201	arg1	ability					2183:2189	the load-bearing ability	2166:2189	the load-bearing ability of the rats	2166:2201	Synovitis scores remained unaffected and histopathologic evaluation of subchondral bone damage did not suggest that 1D treatment changed the load-bearing ability of the rats.
36552848	9	119	theme	mg/kg	1595:1599	arg1	1D					1601:1602	25 mg/kg 1D	1592:1602	25 mg/kg 1D in a paw inflammatory pain model	1592:1635	Co-administering a CB2 antagonists to 25 mg/kg 1D in a paw inflammatory pain model abolished 1D-related anti-swelling effect and partially abolishing its analgesic effects.
36552848	8	120	theme	agonistic	1501:1509	arg1	effects					1511:1517	CB2 agonistic effects	1497:1517	CB2 agonistic effects in cell lines and in chondrocytes	1497:1551	RESULTS The G protein receptor assays confirmed that both 1B and 1D possess CB2 agonistic effects in cell lines and in chondrocytes.
36552848	7	121	theme	post-MMT	1411:1418	arg1	days					1406:1409	28 days post-MMT	1403:1418	28 days post-MMT	1403:1418	The histopathological assessment was carried out according to the Osteoarthritis Research Society International (OARSI) guidelines for rat models following 28 days post-MMT.
36552848	11	122	from	improvement	1890:1900	arg1	degeneration					1915:1926	cartilage degeneration	1905:1926	cartilage degeneration (zonal/total tibial scores)	1905:1954	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	122	from	improvement	1890:1900	arg1	scores					1948:1953	zonal/total tibial scores	1929:1953	zonal/total tibial scores	1929:1953	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	122	from	improvement	1890:1900	arg1	ratios					1973:1978	lesion depth ratios	1960:1978	lesion depth ratios (44%)	1960:1984	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	11	122	from	improvement	1890:1900	arg1	%					1983:1983	44%	1981:1983	44%	1981:1983	Particularly, this dose of 1D exhibited a 30% improvement in cartilage degeneration (zonal/total tibial scores) and lesion depth ratios (44%), comparable to the FGF18 positive control.
36552848	10	123	dep	µg	1771:1772	arg1	i.e.					1762:1765	i.e.	1762:1765	i.e.	1762:1765	Using an MMT model, the high dose (i.e., 24 µg) of 1D administered via IA route, exhibited reduced cartilage damage.
36552848	4	124	theme	CB2	768:770	arg1	selectivity					753:763	the selectivity	749:763	the selectivity of CB2	749:770	METHODS Initially, we assessed the selectivity of CB2 using a Gs-protein receptor cAMP potency assay, which was also validated for antagonistic effects dependent on the Gi-protein receptor cAMP pathway.
36552848	1	125	theme	beneficial	224:233	arg1	effect					235:240	a beneficial effect	222:240	a beneficial effect	222:240	BACKGROUND In a previous report, we have identified the cannabinoid receptor 2 (CB2) agonist HU308 to possess a beneficial effect in preventing age and trauma-induced osteoarthritis (OA) in mice.
35248703	0	0	theme	lipid	90:94	arg1	asymmetry					96:104	outer-membrane lipid asymmetry	75:104	outer-membrane lipid asymmetry	75:104	Pal depletion results in hypervesiculation and affects cell morphology and outer-membrane lipid asymmetry in bordetellae.
35248703	3	1	theme	Novel	351:355	arg1	vaccines					357:364	Novel vaccines	351:364	Novel vaccines	351:364	Novel vaccines could be based on outer-membrane vesicles (OMVs), but vesiculation of bordetellae needs to be increased for cost-effective vaccine production.
35248703	0	2	theme	outer-membrane	75:88	arg1	asymmetry					96:104	outer-membrane lipid asymmetry	75:104	outer-membrane lipid asymmetry	75:104	Pal depletion results in hypervesiculation and affects cell morphology and outer-membrane lipid asymmetry in bordetellae.
35248703	4	3	theme	OMV	540:542	arg1	production					544:553	OMV production	540:553	OMV production	540:553	Here, we focused on increasing OMV production by reducing the anchoring of the outer membrane to the peptidoglycan layer.
35248703	5	4	theme	pal	663:665	arg1	Inactivation					631:642	Inactivation	631:642	Inactivation of rmpM, tolR, and pal	631:665	Inactivation of rmpM, tolR, and pal failed, presumably because their products are essential in bordetellae.
35248703	4	5	theme	membrane	594:601	arg1	anchoring					571:579	the anchoring	567:579	the anchoring of the outer membrane to the peptidoglycan layer	567:628	Here, we focused on increasing OMV production by reducing the anchoring of the outer membrane to the peptidoglycan layer.
35248703	3	6	theme	outer-membrane	384:397	arg1	OMVs					409:412	OMVs	409:412	OMVs	409:412	Novel vaccines could be based on outer-membrane vesicles (OMVs), but vesiculation of bordetellae needs to be increased for cost-effective vaccine production.
35248703	3	6	theme	outer-membrane	384:397	arg1	vesicles					399:406	outer-membrane vesicles	384:406	outer-membrane vesicles (OMVs)	384:413	Novel vaccines could be based on outer-membrane vesicles (OMVs), but vesiculation of bordetellae needs to be increased for cost-effective vaccine production.
35248703	7	7	theme	OMVs	916:919	arg1	composition					901:911	the protein composition	889:911	the protein composition of OMVs produced under Pal-depletion conditions	889:959	SDS-PAGE and Western blot analyses showed that the protein composition of OMVs produced under Pal-depletion conditions resembled that of the outer membrane but differed from that of OMVs released by the wild type.
35248703	4	8	theme	peptidoglycan	610:622	arg1	layer					624:628	the peptidoglycan layer	606:628	the peptidoglycan layer	606:628	Here, we focused on increasing OMV production by reducing the anchoring of the outer membrane to the peptidoglycan layer.
35248703	9	9	from	lipoproteins	1293:1304	arg1	bordetellae					1309:1319	bordetellae	1309:1319	bordetellae	1309:1319	We also identified additional lipoproteins in bordetellae with a putative peptidoglycan-anchoring domain.
35248703	7	10	theme	protein	893:899	arg1	composition					901:911	the protein composition	889:911	the protein composition of OMVs produced under Pal-depletion conditions	889:959	SDS-PAGE and Western blot analyses showed that the protein composition of OMVs produced under Pal-depletion conditions resembled that of the outer membrane but differed from that of OMVs released by the wild type.
35248703	5	11	from	essential	713:721	arg1	bordetellae					726:736	bordetellae	726:736	bordetellae	726:736	Inactivation of rmpM, tolR, and pal failed, presumably because their products are essential in bordetellae.
35248703	11	12	theme	OMV-based	1517:1525	arg1	vaccines					1527:1534	OMV-based vaccines	1517:1534	OMV-based vaccines	1517:1534	We conclude that the conditional pal mutants could be valuable for the development of OMV-based vaccines.
35248703	5	13	theme	rmpM	647:650	arg1	Inactivation					631:642	Inactivation	631:642	Inactivation of rmpM, tolR, and pal	631:665	Inactivation of rmpM, tolR, and pal failed, presumably because their products are essential in bordetellae.
35248703	2	14	theme	vaccines	295:302	arg1	efficacy					283:290	efficacy	283:290	efficacy of vaccines for Bordetella bronchiseptica	283:332	Besides, efficacy of vaccines for Bordetella bronchiseptica remains unclear.
35248703	11	15	theme	vaccines	1527:1534	arg1	development					1502:1512	the development	1498:1512	the development of OMV-based vaccines	1498:1534	We conclude that the conditional pal mutants could be valuable for the development of OMV-based vaccines.
35248703	8	16	theme	Tol-Pal	1210:1216	arg1	system					1218:1223	the Tol-Pal system	1206:1223	the Tol-Pal system	1206:1223	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	8	17	theme	cell	1083:1086	arg1	morphology					1088:1097	the cell morphology	1079:1097	the cell morphology	1079:1097	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	6	18	theme	Pal-depletion	816:828	arg1	conditions					830:839	Pal-depletion conditions	816:839	Pal-depletion conditions	816:839	Conditional pal mutants were constructed, which were hypervesiculating under Pal-depletion conditions.
35248703	8	19	theme	retrograde	1228:1237	arg1	transport					1252:1260	retrograde phospholipid transport	1228:1260	retrograde phospholipid transport	1228:1260	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	11	20	theme	pal	1464:1466	arg1	mutants					1468:1474	the conditional pal mutants	1448:1474	the conditional pal mutants	1448:1474	We conclude that the conditional pal mutants could be valuable for the development of OMV-based vaccines.
35248703	11	20	theme	pal	1464:1466	arg1	valuable					1485:1492	valuable	1485:1492	valuable	1485:1492	We conclude that the conditional pal mutants could be valuable for the development of OMV-based vaccines.
35248703	5	21	theme	tolR	653:656	arg1	Inactivation					631:642	Inactivation	631:642	Inactivation of rmpM, tolR, and pal	631:665	Inactivation of rmpM, tolR, and pal failed, presumably because their products are essential in bordetellae.
35248703	0	22	theme	Pal	0:2	arg1	depletion					4:12	Pal depletion	0:12	Pal depletion	0:12	Pal depletion results in hypervesiculation and affects cell morphology and outer-membrane lipid asymmetry in bordetellae.
35248703	9	23	theme	peptidoglycan-anchoring	1337:1359	arg1	domain					1361:1366	a putative peptidoglycan-anchoring domain	1326:1366	a putative peptidoglycan-anchoring domain	1326:1366	We also identified additional lipoproteins in bordetellae with a putative peptidoglycan-anchoring domain.
35248703	8	24	theme	Pal	1056:1058	arg1	depletion					1060:1068	Pal depletion	1056:1068	Pal depletion	1056:1068	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	3	25	theme	vaccine	489:495	arg1	production					497:506	cost-effective vaccine production	474:506	cost-effective vaccine production	474:506	Novel vaccines could be based on outer-membrane vesicles (OMVs), but vesiculation of bordetellae needs to be increased for cost-effective vaccine production.
35248703	8	26	theme	phospholipid	1239:1250	arg1	transport					1252:1260	retrograde phospholipid transport	1228:1260	retrograde phospholipid transport	1228:1260	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	4	27	theme	outer	588:592	arg1	membrane					594:601	the outer membrane	584:601	the outer membrane	584:601	Here, we focused on increasing OMV production by reducing the anchoring of the outer membrane to the peptidoglycan layer.
35248703	8	28	from	role	1197:1200	arg1	transport					1252:1260	retrograde phospholipid transport	1228:1260	retrograde phospholipid transport	1228:1260	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	9	29	with	lipoproteins	1293:1304	arg1	domain					1361:1366	a putative peptidoglycan-anchoring domain	1326:1366	a putative peptidoglycan-anchoring domain	1326:1366	We also identified additional lipoproteins in bordetellae with a putative peptidoglycan-anchoring domain.
35248703	7	30	theme	Western	855:861	arg1	analyses					868:875	Western blot analyses	855:875	Western blot analyses	855:875	SDS-PAGE and Western blot analyses showed that the protein composition of OMVs produced under Pal-depletion conditions resembled that of the outer membrane but differed from that of OMVs released by the wild type.
35248703	1	31	theme	bacteria	220:227	arg1	transmission					200:211	transmission	200:211	transmission	200:211	Current vaccines against Bordetella pertussis do not prevent colonization and transmission of the bacteria, and vaccine-induced immunity wanes rapidly.
35248703	1	31	theme	bacteria	220:227	arg1	colonization					183:194	colonization	183:194	colonization	183:194	Current vaccines against Bordetella pertussis do not prevent colonization and transmission of the bacteria, and vaccine-induced immunity wanes rapidly.
35248703	7	32	theme	blot	863:866	arg1	analyses					868:875	Western blot analyses	855:875	Western blot analyses	855:875	SDS-PAGE and Western blot analyses showed that the protein composition of OMVs produced under Pal-depletion conditions resembled that of the outer membrane but differed from that of OMVs released by the wild type.
35248703	3	33	theme	bordetellae	436:446	arg1	vesiculation					420:431	vesiculation	420:431	vesiculation of bordetellae	420:446	Novel vaccines could be based on outer-membrane vesicles (OMVs), but vesiculation of bordetellae needs to be increased for cost-effective vaccine production.
35248703	11	34	theme	conditional	1452:1462	arg1	mutants					1468:1474	the conditional pal mutants	1448:1474	the conditional pal mutants	1448:1474	We conclude that the conditional pal mutants could be valuable for the development of OMV-based vaccines.
35248703	11	34	theme	conditional	1452:1462	arg1	valuable					1485:1492	valuable	1485:1492	valuable	1485:1492	We conclude that the conditional pal mutants could be valuable for the development of OMV-based vaccines.
35248703	5	35	from	bordetellae	726:736	arg1	essential					713:721	essential	713:721	essential	713:721	Inactivation of rmpM, tolR, and pal failed, presumably because their products are essential in bordetellae.
35248703	3	36	theme	cost-effective	474:487	arg1	production					497:506	cost-effective vaccine production	474:506	cost-effective vaccine production	474:506	Novel vaccines could be based on outer-membrane vesicles (OMVs), but vesiculation of bordetellae needs to be increased for cost-effective vaccine production.
35248703	0	37	theme	cell	55:58	arg1	morphology					60:69	cell morphology	55:69	cell morphology	55:69	Pal depletion results in hypervesiculation and affects cell morphology and outer-membrane lipid asymmetry in bordetellae.
35248703	8	38	theme	cell-surface-exposed	1139:1158	arg1	phospholipids					1160:1172	cell-surface-exposed phospholipids	1139:1172	cell-surface-exposed phospholipids	1139:1172	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	0	39	from	morphology	60:69	arg1	bordetellae					109:119	bordetellae	109:119	bordetellae	109:119	Pal depletion results in hypervesiculation and affects cell morphology and outer-membrane lipid asymmetry in bordetellae.
35248703	1	40	theme	Current	122:128	arg1	vaccines					130:137	Current vaccines	122:137	Current vaccines against Bordetella pertussis	122:166	Current vaccines against Bordetella pertussis do not prevent colonization and transmission of the bacteria, and vaccine-induced immunity wanes rapidly.
35248703	10	41	theme	OMV	1415:1417	arg1	production					1419:1428	OMV production	1415:1428	OMV production	1415:1428	However, their inactivation did not influence OMV production.
35248703	7	42	theme	outer	983:987	arg1	membrane					989:996	the outer membrane	979:996	the outer membrane	979:996	SDS-PAGE and Western blot analyses showed that the protein composition of OMVs produced under Pal-depletion conditions resembled that of the outer membrane but differed from that of OMVs released by the wild type.
35248703	7	43	theme	wild	1045:1048	arg1	type					1050:1053	the wild type	1041:1053	the wild type	1041:1053	SDS-PAGE and Western blot analyses showed that the protein composition of OMVs produced under Pal-depletion conditions resembled that of the outer membrane but differed from that of OMVs released by the wild type.
35248703	1	44	theme	vaccine-induced	234:248	arg1	immunity					250:257	vaccine-induced immunity	234:257	vaccine-induced immunity	234:257	Current vaccines against Bordetella pertussis do not prevent colonization and transmission of the bacteria, and vaccine-induced immunity wanes rapidly.
35248703	8	45	theme	phospholipids	1160:1172	arg1	amounts					1128:1134	the amounts	1124:1134	the amounts of cell-surface-exposed phospholipids	1124:1172	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	8	45	theme	phospholipids	1160:1172	arg1	phospholipids					1160:1172	cell-surface-exposed phospholipids	1139:1172	cell-surface-exposed phospholipids	1139:1172	Pal depletion affected the cell morphology and appeared to increase the amounts of cell-surface-exposed phospholipids, possibly reflecting a role for the Tol-Pal system in retrograde phospholipid transport.
35248703	9	46	theme	putative	1328:1335	arg1	domain					1361:1366	a putative peptidoglycan-anchoring domain	1326:1366	a putative peptidoglycan-anchoring domain	1326:1366	We also identified additional lipoproteins in bordetellae with a putative peptidoglycan-anchoring domain.
35248703	1	47	theme	Bordetella	147:156	arg1	pertussis					158:166	Bordetella pertussis	147:166	Bordetella pertussis	147:166	Current vaccines against Bordetella pertussis do not prevent colonization and transmission of the bacteria, and vaccine-induced immunity wanes rapidly.
35248703	7	48	theme	Pal-depletion	936:948	arg1	conditions					950:959	Pal-depletion conditions	936:959	Pal-depletion conditions	936:959	SDS-PAGE and Western blot analyses showed that the protein composition of OMVs produced under Pal-depletion conditions resembled that of the outer membrane but differed from that of OMVs released by the wild type.
35248703	0	49	from	asymmetry	96:104	arg1	bordetellae					109:119	bordetellae	109:119	bordetellae	109:119	Pal depletion results in hypervesiculation and affects cell morphology and outer-membrane lipid asymmetry in bordetellae.
35248703	9	50	theme	additional	1282:1291	arg1	lipoproteins					1293:1304	additional lipoproteins	1282:1304	additional lipoproteins in bordetellae with a putative peptidoglycan-anchoring domain	1282:1366	We also identified additional lipoproteins in bordetellae with a putative peptidoglycan-anchoring domain.
35248703	6	51	theme	pal	751:753	arg1	mutants					755:761	Conditional pal mutants	739:761	Conditional pal mutants	739:761	Conditional pal mutants were constructed, which were hypervesiculating under Pal-depletion conditions.
35248703	6	52	theme	Conditional	739:749	arg1	mutants					755:761	Conditional pal mutants	739:761	Conditional pal mutants	739:761	Conditional pal mutants were constructed, which were hypervesiculating under Pal-depletion conditions.
37336380	6	0	theme	stimuli-responsive	954:971	arg1	release					978:984	stimuli-responsive drug release	954:984	stimuli-responsive drug release	954:984	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	6	1	theme	loading	942:948	arg1	efficiency					986:995	the drug loading and stimuli-responsive drug release efficiency	933:995	the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system	933:1017	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	2	2	theme	complex-based	318:330	arg1	hydrogel					332:339	polyelectrolyte complex-based hydrogel	302:339	integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	291:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	2	2	theme	complex-based	318:330	arg1	HG					347:348	MPEC HG	342:348	MPEC HG	342:348	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	1	3	theme	dual	129:132	arg1	system					167:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	7	4	theme	loading	1147:1153	arg1	%					1172:1172	~ 68 %	1167:1172	~ 68 %	1167:1172	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	4	theme	loading	1147:1153	arg1	efficiency					1155:1164	great drug loading efficiency	1136:1164	great drug loading efficiency (~ 68 %)	1136:1173	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	2	5	theme	integrated	291:300	arg1	system					351:356	integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	291:356	integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	291:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	9	6	theme	HG	1416:1417	arg1	system					1419:1424	the MPEC HG system	1407:1424	the MPEC HG system	1407:1424	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	9	6	theme	HG	1416:1417	arg1	result					1399:1404	a result	1397:1404	a result	1397:1404	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	4	7	theme	sulfonate	713:721	arg1	groups					723:728	negatively charged sulfonate groups	694:728	negatively charged sulfonate groups	694:728	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	7	8	theme	great	1136:1140	arg1	%					1172:1172	~ 68 %	1167:1172	~ 68 %	1167:1172	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	8	theme	great	1136:1140	arg1	efficiency					1155:1164	great drug loading efficiency	1136:1164	great drug loading efficiency (~ 68 %)	1136:1173	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	8	9	theme	HG	1224:1225	arg1	system					1227:1232	the MPEC HG system	1215:1232	the MPEC HG system's in vitro cytotoxicity behavior	1215:1265	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	6	10	theme	system	1012:1017	arg1	efficiency					986:995	the drug loading and stimuli-responsive drug release efficiency	933:995	the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system	933:1017	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	4	11	theme	charged	666:672	arg1	units					684:688	positively charged guanidine units	655:688	positively charged guanidine units	655:688	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	1	12	theme	drug	220:223	arg1	delivery					225:232	controlled drug delivery	209:232	controlled drug delivery to specific sites	209:250	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	6	13	theme	MPEC	1004:1007	arg1	system					1012:1017	the MPEC HG system	1000:1017	the MPEC HG system	1000:1017	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	8	14	theme	liver	1295:1299	arg1	line					1321:1324	a human liver cancer (HepG2) cell line	1287:1324	a human liver cancer (HepG2) cell line	1287:1324	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	7	15	theme	developed	1097:1105	arg1	system					1116:1121	the developed hydrogel system	1093:1121	the developed hydrogel system	1093:1121	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	16	theme	drug	1188:1191	arg1	release					1193:1199	drug release	1188:1199	drug release	1188:1199	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	17	theme	stimuli	1058:1064	arg1	pH 5.0/42 °C					1078:1089	pH 5.0/42 °C	1078:1089	pH 5.0/42 °C	1078:1089	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	17	theme	stimuli	1058:1064	arg1	conditions					1066:1075	the combined pH and temperature stimuli conditions	1026:1075	the combined pH and temperature stimuli conditions (pH 5.0/42 °C)	1026:1090	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	9	18	theme	dual	1444:1447	arg1	pH					1449:1450	dual pH	1444:1450	dual pH	1444:1450	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	1	19	theme	stimuli-responsive	134:151	arg1	system					167:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	6	20	theme	release	978:984	arg1	efficiency					986:995	the drug loading and stimuli-responsive drug release efficiency	933:995	the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system	933:1017	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	5	21	theme	characterization	739:754	arg1	approaches					756:765	Various characterization approaches	731:765	Various characterization approaches	731:765	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	7	22	theme	combined	1030:1037	arg1	pH					1039:1040	combined pH	1030:1040	combined pH	1030:1040	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	9	23	theme	stimuli-responsive	1468:1485	arg1	delivery					1492:1499	stimuli-responsive drug delivery	1468:1499	stimuli-responsive drug delivery	1468:1499	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	9	24	theme	temperature	1456:1466	arg1	applications					1501:1512	dual pH and temperature stimuli-responsive drug delivery applications	1444:1512	dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy	1444:1530	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	5	25	theme	complex	832:838	arg1	hydrogel					840:847	magnetic polyelectrolyte complex hydrogel	807:847	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	5	25	theme	complex	832:838	arg1	HG					855:856	MPEC HG	850:856	MPEC HG	850:856	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	2	26	theme	magnetic	257:264	arg1	oxide					271:275	The magnetic iron oxide	253:275	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	253:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	4	27	theme	natural	516:522	arg1	kCG					550:552	kCG	550:552	kCG	550:552	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	4	27	theme	natural	516:522	arg1	k-carrageenan					535:547	the natural biopolymer k-carrageenan	512:547	the natural biopolymer k-carrageenan (kCG)	512:553	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	0	28	theme	curcumin	106:113	arg1	delivery					115:122	temperature-responsive curcumin delivery	83:122	temperature-responsive curcumin delivery	83:122	k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.
37336380	1	29	theme	delivery	158:165	arg1	system					167:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	9	30	dep	pH	1449:1450	arg1	delivery					1492:1499	stimuli-responsive drug delivery	1468:1499	stimuli-responsive drug delivery	1468:1499	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	3	31	theme	polymer	455:461	arg1	PHMG					492:495	PHMG	492:495	PHMG	492:495	First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG).
37336380	3	31	theme	polymer	455:461	arg1	guanidine					481:489	the synthetic polymer polyhexamethylene guanidine	441:489	the synthetic polymer polyhexamethylene guanidine (PHMG)	441:496	First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG).
37336380	5	32	theme	magnetic	807:814	arg1	hydrogel					840:847	magnetic polyelectrolyte complex hydrogel	807:847	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	5	32	theme	magnetic	807:814	arg1	HG					855:856	MPEC HG	850:856	MPEC HG	850:856	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	0	33	theme	complex	45:51	arg1	hydrogel					63:70	magnetic@polyelectrolyte complex composite hydrogel	20:70	magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery	20:122	k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.
37336380	9	34	used	used	1435:1438	arg2	result					1399:1404	a result	1397:1404	a result	1397:1404	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	9	34	used	used	1435:1438	arg2	system					1419:1424	the MPEC HG system	1407:1424	the MPEC HG system	1407:1424	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	8	35	theme	MPEC	1361:1364	arg1	biocompatible					1379:1391	biocompatible	1379:1391	biocompatible	1379:1391	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	8	35	theme	MPEC	1361:1364	arg1	system					1369:1374	the MPEC HG system	1357:1374	the MPEC HG system	1357:1374	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	0	36	theme	magnetic	20:27	arg1	hydrogel					63:70	magnetic@polyelectrolyte complex composite hydrogel	20:70	magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery	20:122	k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.
37336380	0	37	theme	polyelectrolyte	29:43	arg1	hydrogel					63:70	magnetic@polyelectrolyte complex composite hydrogel	20:70	magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery	20:122	k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.
37336380	6	38	theme	model	900:904	arg1	Curcumin					867:874	Curcumin	867:874	Curcumin (Cur)	867:880	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	6	38	theme	model	900:904	arg1	agent					916:920	a model bioactive agent	898:920	a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system	898:1017	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	0	39	theme	composite	53:61	arg1	hydrogel					63:70	magnetic@polyelectrolyte complex composite hydrogel	20:70	magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery	20:122	k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.
37336380	1	40	theme	specific	237:244	arg1	sites					246:250	specific sites	237:250	specific sites	237:250	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	6	41	theme	drug	973:976	arg1	release					978:984	stimuli-responsive drug release	954:984	stimuli-responsive drug release	954:984	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	5	42	theme	hydrogel	840:847	arg1	system					859:864	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	7	43	theme	~	1167:1167	arg1	%					1172:1172	~ 68 %	1167:1172	~ 68 %	1167:1172	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	43	theme	~	1167:1167	arg1	efficiency					1155:1164	great drug loading efficiency	1136:1164	great drug loading efficiency (~ 68 %)	1136:1173	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	9	44	from	applications	1501:1512	arg1	therapy					1524:1530	cancer therapy	1517:1530	cancer therapy	1517:1530	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	2	45	theme	hydrogel	332:339	arg1	system					351:356	integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	291:356	integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	291:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	6	46	theme	drug	937:940	arg1	loading					942:948	drug loading	937:948	drug loading	937:948	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	7	47	theme	drug	1142:1145	arg1	%					1172:1172	~ 68 %	1167:1172	~ 68 %	1167:1172	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	47	theme	drug	1142:1145	arg1	efficiency					1155:1164	great drug loading efficiency	1136:1164	great drug loading efficiency (~ 68 %)	1136:1173	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	2	48	theme	polyelectrolyte	302:316	arg1	hydrogel					332:339	polyelectrolyte complex-based hydrogel	302:339	integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	291:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	2	48	theme	polyelectrolyte	302:316	arg1	HG					347:348	MPEC HG	342:348	MPEC HG	342:348	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	9	49	theme	MPEC	1411:1414	arg1	system					1419:1424	the MPEC HG system	1407:1424	the MPEC HG system	1407:1424	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	9	49	theme	MPEC	1411:1414	arg1	result					1399:1404	a result	1397:1404	a result	1397:1404	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	8	50	theme	cytotoxicity	1245:1256	arg1	behavior					1258:1265	the MPEC HG system's in vitro cytotoxicity behavior	1215:1265	the MPEC HG system's in vitro cytotoxicity behavior	1215:1265	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	1	51	theme	interest	197:204	arg1	interest					197:204	interest	197:204	interest	197:204	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	1	51	theme	interest	197:204	arg1	lot					190:192	a lot	188:192	a lot of interest	188:204	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	2	52	dep	oxide	271:275	arg1	nanoparticles					277:289	nanoparticles	277:289	nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	277:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	7	53	theme	hydrogel	1107:1114	arg1	system					1116:1121	the developed hydrogel system	1093:1121	the developed hydrogel system	1093:1121	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	2	54	theme	MPEC	342:345	arg1	hydrogel					332:339	polyelectrolyte complex-based hydrogel	302:339	integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	291:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	2	54	theme	MPEC	342:345	arg1	HG					347:348	MPEC HG	342:348	MPEC HG	342:348	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	1	55	theme	controlled	209:218	arg1	delivery					225:232	controlled drug delivery	209:232	controlled drug delivery to specific sites	209:250	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	8	56	theme	MPEC	1219:1222	arg1	system					1227:1232	the MPEC HG system	1215:1232	the MPEC HG system's in vitro cytotoxicity behavior	1215:1265	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	6	57	theme	HG	1009:1010	arg1	system					1012:1017	the MPEC HG system	1000:1017	the MPEC HG system	1000:1017	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	4	58	theme	charged	705:711	arg1	groups					723:728	negatively charged sulfonate groups	694:728	negatively charged sulfonate groups	694:728	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	5	59	used	used	772:775	arg2	approaches					756:765	Various characterization approaches	731:765	Various characterization approaches	731:765	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	4	60	theme	guanidine	674:682	arg1	units					684:688	positively charged guanidine units	655:688	positively charged guanidine units	655:688	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	9	61	theme	pH	1449:1450	arg1	applications					1501:1512	dual pH and temperature stimuli-responsive drug delivery applications	1444:1512	dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy	1444:1530	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	8	62	theme	in	1236:1237	arg1	cytotoxicity					1245:1256	in vitro cytotoxicity	1236:1256	the MPEC HG system's in vitro cytotoxicity behavior	1215:1265	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	8	63	theme	cancer	1301:1306	arg1	line					1321:1324	a human liver cancer (HepG2) cell line	1287:1324	a human liver cancer (HepG2) cell line	1287:1324	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	5	64	theme	Various	731:737	arg1	approaches					756:765	Various characterization approaches	731:765	Various characterization approaches	731:765	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	7	65	theme	temperature	1046:1056	arg1	stimuli					1058:1064	temperature stimuli	1046:1064	temperature stimuli	1046:1064	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	1	66	theme	drug	153:156	arg1	system					167:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system	125:172	The dual stimuli-responsive drug delivery system has attracted a lot of interest in controlled drug delivery to specific sites.
37336380	8	67	theme	HepG2	1309:1313	arg1	line					1321:1324	a human liver cancer (HepG2) cell line	1287:1324	a human liver cancer (HepG2) cell line	1287:1324	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	5	68	theme	polyelectrolyte	816:830	arg1	hydrogel					840:847	magnetic polyelectrolyte complex hydrogel	807:847	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	5	68	theme	polyelectrolyte	816:830	arg1	HG					855:856	MPEC HG	850:856	MPEC HG	850:856	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	2	69	theme	iron	266:269	arg1	oxide					271:275	The magnetic iron oxide	253:275	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system	253:356	The magnetic iron oxide nanoparticles integrated polyelectrolyte complex-based hydrogel (MPEC HG) system was developed in this work.
37336380	7	70	theme	pH	1039:1040	arg1	pH 5.0/42 °C					1078:1089	pH 5.0/42 °C	1078:1089	pH 5.0/42 °C	1078:1089	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	7	70	theme	pH	1039:1040	arg1	conditions					1066:1075	the combined pH and temperature stimuli conditions	1026:1075	the combined pH and temperature stimuli conditions (pH 5.0/42 °C)	1026:1090	Under the combined pH and temperature stimuli conditions (pH 5.0/42 °C), the developed hydrogel system demonstrated great drug loading efficiency (~ 68 %) and enhanced drug release.
37336380	9	71	theme	drug	1487:1490	arg1	delivery					1492:1499	stimuli-responsive drug delivery	1468:1499	stimuli-responsive drug delivery	1468:1499	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	4	72	theme	biopolymer	524:533	arg1	kCG					550:552	kCG	550:552	kCG	550:552	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	4	72	theme	biopolymer	524:533	arg1	k-carrageenan					535:547	the natural biopolymer k-carrageenan	512:547	the natural biopolymer k-carrageenan (kCG)	512:553	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	8	73	theme	cell	1316:1319	arg1	line					1321:1324	a human liver cancer (HepG2) cell line	1287:1324	a human liver cancer (HepG2) cell line	1287:1324	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	0	74	theme	temperature-responsive	83:104	arg1	delivery					115:122	temperature-responsive curcumin delivery	83:122	temperature-responsive curcumin delivery	83:122	k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.
37336380	9	75	theme	cancer	1517:1522	arg1	therapy					1524:1530	cancer therapy	1517:1530	cancer therapy	1517:1530	As a result, the MPEC HG system might be used for dual pH and temperature stimuli-responsive drug delivery applications in cancer therapy.
37336380	5	76	theme	developed	797:805	arg1	system					859:864	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	3	77	theme	polyhexamethylene	463:479	arg1	PHMG					492:495	PHMG	492:495	PHMG	492:495	First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG).
37336380	3	77	theme	polyhexamethylene	463:479	arg1	guanidine					481:489	the synthetic polymer polyhexamethylene guanidine	441:489	the synthetic polymer polyhexamethylene guanidine (PHMG)	441:496	First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG).
37336380	3	78	theme	synthetic	445:453	arg1	PHMG					492:495	PHMG	492:495	PHMG	492:495	First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG).
37336380	3	78	theme	synthetic	445:453	arg1	guanidine					481:489	the synthetic polymer polyhexamethylene guanidine	441:489	the synthetic polymer polyhexamethylene guanidine (PHMG)	441:496	First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG).
37336380	0	79	theme	@	28:28	arg1	hydrogel					63:70	magnetic@polyelectrolyte complex composite hydrogel	20:70	magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery	20:122	k-Carrageenan based magnetic@polyelectrolyte complex composite hydrogel for pH and temperature-responsive curcumin delivery.
37336380	3	80	theme	magnetic	393:400	arg1	nanoparticles					402:414	magnetic nanoparticles	393:414	magnetic nanoparticles	393:414	First, magnetic nanoparticles were produced in situ in the synthetic polymer polyhexamethylene guanidine (PHMG).
37336380	4	81	theme	polyelectrolyte	580:594	arg1	PEC					605:607	PEC	605:607	PEC	605:607	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	4	81	theme	polyelectrolyte	580:594	arg1	complex					596:602	the polyelectrolyte complex	576:602	the polyelectrolyte complex (PEC)	576:608	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	8	82	dep	in	1236:1237	arg1	vitro					1239:1243	vitro	1239:1243	vitro	1239:1243	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	8	83	theme	HG	1366:1367	arg1	biocompatible					1379:1391	biocompatible	1379:1391	biocompatible	1379:1391	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	8	83	theme	HG	1366:1367	arg1	system					1369:1374	the MPEC HG system	1357:1374	the MPEC HG system	1357:1374	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
37336380	5	84	theme	MPEC	850:853	arg1	hydrogel					840:847	magnetic polyelectrolyte complex hydrogel	807:847	the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system	793:864	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	5	84	theme	MPEC	850:853	arg1	HG					855:856	MPEC HG	850:856	MPEC HG	850:856	Various characterization approaches were used to characterize the developed magnetic polyelectrolyte complex hydrogel (MPEC HG) system.
37336380	6	85	theme	bioactive	906:914	arg1	Curcumin					867:874	Curcumin	867:874	Curcumin (Cur)	867:880	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	6	85	theme	bioactive	906:914	arg1	agent					916:920	a model bioactive agent	898:920	a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system	898:1017	Curcumin (Cur) was employed as a model bioactive agent to examine the drug loading and stimuli-responsive drug release efficiency of the MPEC HG system.
37336380	4	86	theme	charge-balancing	618:633	arg1	interaction					635:645	charge-balancing interaction	618:645	charge-balancing interaction between positively charged guanidine units	618:688	Furthermore, the natural biopolymer k-carrageenan (kCG) was employed to form the polyelectrolyte complex (PEC) through charge-balancing interaction between positively charged guanidine units and negatively charged sulfonate groups.
37336380	8	87	theme	human	1289:1293	arg1	line					1321:1324	a human liver cancer (HepG2) cell line	1287:1324	a human liver cancer (HepG2) cell line	1287:1324	Furthermore, the MPEC HG system's in vitro cytotoxicity behavior was investigated on a human liver cancer (HepG2) cell line, and the results revealed that the MPEC HG system is biocompatible.
36444094	2	0	theme	microbiota	427:436	arg1	influence					410:418	the influence	406:418	the influence of gut microbiota and serum metabolic profile	406:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	5	1	theme	metabolic	1075:1083	arg1	function					1085:1092	metabolic function	1075:1092	metabolic function	1075:1092	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	2	2	theme	foxtail	307:313	arg1	starch					322:327	heat-treated foxtail millet starch	294:327	heat-treated foxtail millet starch	294:327	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	1	3	theme	protein	202:208	arg1	role					183:186	the role	179:186	the role of starch and protein in millet in these effects	179:235	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	1	3	theme	protein	202:208	arg1	unclear					240:246	unclear	240:246	unclear	240:246	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	2	4	theme	gut	423:425	arg1	microbiota					427:436	gut microbiota	423:436	gut microbiota	423:436	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	4	5	theme	probiotics	778:787	arg1	abundance					765:773	the abundance	761:773	the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration	761:919	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	0	6	from	millet	56:61	arg1	protein					35:41	protein	35:41	protein	35:41	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	0	6	from	millet	56:61	arg1	Effects					0:6	Effects	0:6	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.	0:104	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	0	6	from	millet	56:61	arg1	starch					24:29	starch	24:29	starch	24:29	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	4	7	theme	reduced	837:843	arg1	bacteria					853:860	reduced harmful bacteria	837:860	reduced harmful bacteria	837:860	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	0	8	from	Effects	0:6	arg1	millet					56:61	foxtail millet	48:61	foxtail millet (Setaria italica)	48:79	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	0	8	from	Effects	0:6	arg1	italica					72:78	Setaria italica	64:78	Setaria italica	64:78	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	0	8	from	Effects	0:6	arg1	mice					100:103	type 2 diabetic mice	84:103	type 2 diabetic mice	84:103	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	4	9	theme	fatty	805:809	arg1	acids					811:815	short-chain fatty acids	793:815	short-chain fatty acids	793:815	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	4	10	theme	foxtail	678:684	arg1	starch					693:698	heat-treated foxtail millet starch	665:698	heat-treated foxtail millet starch	665:698	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	5	11	theme	millet	1038:1043	arg1	starch					1045:1050	heat-treated foxtail millet starch	1017:1050	heat-treated foxtail millet starch	1017:1050	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	4	12	theme	short-chain	793:803	arg1	acids					811:815	short-chain fatty acids	793:815	short-chain fatty acids	793:815	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	6	13	theme	serum	1234:1238	arg1	profile					1250:1256	serum metabolic profile	1234:1256	serum metabolic profile	1234:1256	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	4	14	theme	heat-treated	665:676	arg1	starch					693:698	heat-treated foxtail millet starch	665:698	heat-treated foxtail millet starch	665:698	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	3	15	theme	foxtail	517:523	arg1	starch					532:537	heat-treated foxtail millet starch	504:537	heat-treated foxtail millet starch	504:537	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	2	16	theme	profile	458:464	arg1	influence					410:418	the influence	406:418	the influence of gut microbiota and serum metabolic profile	406:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	3	17	dep	glucose	588:594	arg1	%					601:601	18.52%	596:601	18.52%	596:601	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	3	17	dep	glucose	588:594	arg1	%					612:612	26.33%	607:612	26.33%	607:612	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	2	18	from	impact	284:289	arg1	mechanisms					384:393	the underlying mechanisms	369:393	the underlying mechanisms	369:393	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	2	18	from	impact	284:289	arg1	influence					410:418	the influence	406:418	the influence of gut microbiota and serum metabolic profile	406:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	2	18	from	impact	284:289	arg1	mice					360:363	type 2 diabetic mice	344:363	type 2 diabetic mice	344:363	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	3	19	theme	diabetic	470:477	arg1	mice					479:482	diabetic mice	470:482	diabetic mice	470:482	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	4	20	theme	gut	724:726	arg1	composition					739:749	the gut microbiota composition	720:749	the gut microbiota composition	720:749	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	2	21	theme	metabolic	448:456	arg1	profile					458:464	serum metabolic profile	442:464	serum metabolic profile	442:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	5	22	theme	greater	1056:1062	arg1	effects					1064:1070	greater effects	1056:1070	greater effects	1056:1070	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	6	23	theme	microbiota	1219:1228	arg1	modulation					1196:1205	the modulation	1192:1205	the modulation of both gut microbiota and serum metabolic profile	1192:1256	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	4	24	theme	millet	686:691	arg1	starch					693:698	heat-treated foxtail millet starch	665:698	heat-treated foxtail millet starch	665:698	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	1	25	theme	hypoglycemic	145:156	arg1	effects					158:164	hypoglycemic effects	145:164	hypoglycemic effects on mice	145:172	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	5	26	theme	greater	962:968	arg1	effects					970:976	greater effects	962:976	greater effects	962:976	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	0	27	theme	Setaria	64:70	arg1	millet					56:61	foxtail millet	48:61	foxtail millet (Setaria italica)	48:79	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	0	27	theme	Setaria	64:70	arg1	italica					72:78	Setaria italica	64:78	Setaria italica	64:78	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	6	28	theme	gut	1215:1217	arg1	microbiota					1219:1228	gut microbiota	1215:1228	gut microbiota	1215:1228	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	4	29	theme	acids	901:905	arg1	concentration					907:919	increased fecal short-chain fatty acids concentration	867:919	increased fecal short-chain fatty acids concentration	867:919	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	3	30	theme	heat-treated	504:515	arg1	starch					532:537	heat-treated foxtail millet starch	504:537	heat-treated foxtail millet starch	504:537	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	2	31	theme	protein	333:339	arg1	impact					284:289	the impact	280:289	the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile	280:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	2	32	theme	present	253:259	arg1	study					261:265	The present study	249:265	The present study	249:265	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	6	33	theme	profile	1250:1256	arg1	modulation					1196:1205	the modulation	1192:1205	the modulation of both gut microbiota and serum metabolic profile	1192:1256	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	0	34	theme	starch	24:29	arg1	Effects					0:6	Effects	0:6	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.	0:104	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	4	35	theme	harmful	845:851	arg1	bacteria					853:860	reduced harmful bacteria	837:860	reduced harmful bacteria	837:860	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	5	36	theme	gut	981:983	arg1	composition					996:1006	gut microbiota composition	981:1006	gut microbiota composition	981:1006	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	0	37	theme	heat-treated	11:22	arg1	starch					24:29	starch	24:29	starch	24:29	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	3	38	dep	levels	626:631	arg1	%					638:638	12.22%	633:638	12.22%	633:638	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	3	38	dep	levels	626:631	arg1	%					649:649	15.96%	644:649	15.96%	644:649	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	0	39	theme	diabetic	91:98	arg1	mice					100:103	type 2 diabetic mice	84:103	type 2 diabetic mice	84:103	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	2	40	theme	heat-treated	294:305	arg1	starch					322:327	heat-treated foxtail millet starch	294:327	heat-treated foxtail millet starch	294:327	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	0	41	theme	protein	35:41	arg1	Effects					0:6	Effects	0:6	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.	0:104	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	5	42	theme	heat-treated	1017:1028	arg1	starch					1045:1050	heat-treated foxtail millet starch	1017:1050	heat-treated foxtail millet starch	1017:1050	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	4	43	theme	microbiota	728:737	arg1	composition					739:749	the gut microbiota composition	720:749	the gut microbiota composition	720:749	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	2	44	theme	diabetic	351:358	arg1	influence					410:418	the influence	406:418	the influence of gut microbiota and serum metabolic profile	406:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	2	44	theme	diabetic	351:358	arg1	mice					360:363	type 2 diabetic mice	344:363	type 2 diabetic mice	344:363	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	5	45	theme	foxtail	1030:1036	arg1	starch					1045:1050	heat-treated foxtail millet starch	1017:1050	heat-treated foxtail millet starch	1017:1050	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	2	46	theme	underlying	373:382	arg1	mechanisms					384:393	the underlying mechanisms	369:393	the underlying mechanisms	369:393	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	2	46	theme	underlying	373:382	arg1	influence					410:418	the influence	406:418	the influence of gut microbiota and serum metabolic profile	406:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	4	47	theme	fecal	877:881	arg1	acids					901:905	fecal short-chain fatty acids	877:905	increased fecal short-chain fatty acids concentration	867:919	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	0	48	theme	foxtail	48:54	arg1	millet					56:61	foxtail millet	48:61	foxtail millet (Setaria italica)	48:79	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	0	48	theme	foxtail	48:54	arg1	italica					72:78	Setaria italica	64:78	Setaria italica	64:78	Effects of heat-treated starch and protein from foxtail millet (Setaria italica) on type 2 diabetic mice.
36444094	4	49	theme	increased	867:875	arg1	concentration					907:919	increased fecal short-chain fatty acids concentration	867:919	increased fecal short-chain fatty acids concentration	867:919	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	5	50	contain	had	1052:1054	arg1	starch					1045:1050	heat-treated foxtail millet starch	1017:1050	heat-treated foxtail millet starch	1017:1050	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	5	50	contain	had	1052:1054	arg2	effects					1064:1070	greater effects	1056:1070	greater effects	1056:1070	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	1	51	contain	have	140:143	arg1	components					129:138	its components	125:138	its components	125:138	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	1	51	contain	have	140:143	arg1	millet					114:119	Foxtail millet	106:119	Foxtail millet	106:119	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	1	51	contain	have	140:143	arg2	effects					158:164	hypoglycemic effects	145:164	hypoglycemic effects on mice	145:172	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	3	52	theme	millet	525:530	arg1	starch					532:537	heat-treated foxtail millet starch	504:537	heat-treated foxtail millet starch	504:537	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	3	53	theme	insulin	618:624	arg1	levels					626:631	insulin levels	618:631	insulin levels 12.22% and 15.96%	618:649	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	6	54	theme	millet	1146:1151	arg1	potential					1112:1120	The hypoglycemic potential	1095:1120	The hypoglycemic potential of heat-treated foxtail millet starch and protein	1095:1170	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	4	55	theme	acids	811:815	arg1	abundance					765:773	the abundance	761:773	the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration	761:919	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	3	56	theme	blood	582:586	arg1	glucose					588:594	blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%	582:649	glucose	588:594	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	3	57	theme	starch	532:537	arg1	consumption					489:499	the consumption	485:499	the consumption of heat-treated foxtail millet starch and protein	485:549	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	6	58	theme	metabolic	1240:1248	arg1	profile					1250:1256	serum metabolic profile	1234:1256	serum metabolic profile	1234:1256	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	4	59	theme	short-chain	883:893	arg1	acids					901:905	fecal short-chain fatty acids	877:905	increased fecal short-chain fatty acids concentration	867:919	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	1	60	from	role	183:186	arg1	millet					213:218	millet	213:218	millet	213:218	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	1	60	from	role	183:186	arg1	effects					229:235	these effects	223:235	these effects	223:235	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	5	61	theme	microbiota	985:994	arg1	composition					996:1006	gut microbiota composition	981:1006	gut microbiota composition	981:1006	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	5	62	theme	Heat-treated	922:933	arg1	protein					950:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	3	63	theme	protein	543:549	arg1	consumption					489:499	the consumption	485:499	the consumption of heat-treated foxtail millet starch and protein	485:549	In diabetic mice, the consumption of heat-treated foxtail millet starch and protein reduced, respectively, fasting blood glucose 18.52% and 26.33% and insulin levels 12.22% and 15.96%.
36444094	6	64	theme	hypoglycemic	1099:1110	arg1	potential					1112:1120	The hypoglycemic potential	1095:1120	The hypoglycemic potential of heat-treated foxtail millet starch and protein	1095:1170	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	6	65	theme	heat-treated	1125:1136	arg1	millet					1146:1151	heat-treated foxtail millet starch and protein	1125:1170	heat-treated foxtail millet starch and protein	1125:1170	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	5	66	theme	foxtail	935:941	arg1	protein					950:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	1	67	from	effects	158:164	arg1	mice					169:172	mice	169:172	mice	169:172	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	1	68	theme	Foxtail	106:112	arg1	millet					114:119	Foxtail millet	106:119	Foxtail millet	106:119	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	2	69	theme	serum	442:446	arg1	profile					458:464	serum metabolic profile	442:464	serum metabolic profile	442:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	6	70	dep	millet	1146:1151	arg1	protein					1164:1170	protein	1164:1170	protein	1164:1170	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	6	70	dep	millet	1146:1151	arg1	starch					1153:1158	starch	1153:1158	starch	1153:1158	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	5	71	theme	millet	943:948	arg1	protein					950:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	6	72	theme	foxtail	1138:1144	arg1	millet					1146:1151	heat-treated foxtail millet starch and protein	1125:1170	heat-treated foxtail millet starch and protein	1125:1170	The hypoglycemic potential of heat-treated foxtail millet starch and protein was associated with the modulation of both gut microbiota and serum metabolic profile.
36444094	2	73	theme	starch	322:327	arg1	impact					284:289	the impact	280:289	the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile	280:464	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
36444094	5	74	contain	had	958:960	arg1	protein					950:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein	922:956	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	5	74	contain	had	958:960	arg2	effects					970:976	greater effects	962:976	greater effects	962:976	Heat-treated foxtail millet protein had greater effects on gut microbiota composition, whereas heat-treated foxtail millet starch had greater effects on metabolic function.
36444094	1	75	theme	starch	191:196	arg1	role					183:186	the role	179:186	the role of starch and protein in millet in these effects	179:235	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	1	75	theme	starch	191:196	arg1	unclear					240:246	unclear	240:246	unclear	240:246	Foxtail millet and its components have hypoglycemic effects on mice, but the role of starch and protein in millet in these effects is unclear.
36444094	4	76	theme	fatty	895:899	arg1	acids					901:905	fecal short-chain fatty acids	877:905	increased fecal short-chain fatty acids concentration	867:919	In addition, heat-treated foxtail millet starch and protein altered the gut microbiota composition, enriched the abundance of probiotics and short-chain fatty acids producing bacteria, reduced harmful bacteria, and increased fecal short-chain fatty acids concentration.
36444094	2	77	theme	millet	315:320	arg1	starch					322:327	heat-treated foxtail millet starch	294:327	heat-treated foxtail millet starch	294:327	The present study investigated the impact of heat-treated foxtail millet starch and protein on type 2 diabetic mice and the underlying mechanisms, including the influence of gut microbiota and serum metabolic profile.
37148934	4	0	theme	surface	676:682	arg1	morphology					684:693	surface morphology	676:693	surface morphology	676:693	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	3	1	theme	VB	501:502	arg1	effects					490:496	The effects	486:496	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water,	486:638	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	5	2	theme	Young	973:977	arg1	modulus					981:987	Young's modulus	973:987	Young's modulus	973:987	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	1	3	theme	bentonite	275:283	arg1	clay					285:288	bentonite clay	275:288	bentonite clay (BC)	275:293	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	1	3	theme	bentonite	275:283	arg1	BC					291:292	BC	291:292	BC	291:292	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	5	4	dep	strength	960:967	arg1	the					948:950	the	948:950	the	948:950	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	4	5	theme	nanocomposites	865:878	arg1	relationship					845:856	the structure-property relationship	822:856	the structure-property relationship of the nanocomposites	822:878	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	3	6	from	loss	625:628	arg1	water					633:637	water	633:637	water	633:637	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	4	7	theme	SEM	775:777	arg1	microscopy					779:788	high-resolution SEM microscopy	759:788	high-resolution SEM microscopy	759:788	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	5	8	theme	highest	1015:1021	arg1	values					1023:1028	the highest values	1011:1028	the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC	1011:1095	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	3	9	theme	TPS/BC	541:546	arg1	loss					625:628	weight loss	618:628	weight loss in water	618:637	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	3	9	theme	TPS/BC	541:546	arg1	films					548:552	TPS/BC films	541:552	TPS/BC films	541:552	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	3	9	theme	TPS/BC	541:546	arg1	properties					588:597	mechanical and thermal properties	565:597	mechanical and thermal properties	565:597	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	3	9	theme	TPS/BC	541:546	arg1	uptake					606:611	water uptake	600:611	water uptake	600:611	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	4	10	dep	morphology	684:693	arg1	the					672:674	the	672:674	the	672:674	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	0	11	theme	release	114:120	arg1	behavior					122:129	release behavior	114:129	release behavior	114:129	Thermoplastic starch/bentonite clay nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior.
37148934	7	12	theme	biopolymer	1435:1444	arg1	industry					1446:1453	the biopolymer industry	1431:1453	the biopolymer industry	1431:1453	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	3	13	theme	films	548:552	arg1	properties					527:536	the physicochemical properties	507:536	the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water,	507:638	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	1	14	dep	development	156:166	arg1	the					152:154	the	152:154	the	152:154	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	3	15	from	uptake	606:611	arg1	water					633:637	water	633:637	water	633:637	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	4	16	theme	structure-property	826:843	arg1	relationship					845:856	the structure-property relationship	822:856	the structure-property relationship of the nanocomposites	822:878	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	7	17	theme	TPS/BC/VB	1259:1267	arg1	nanocomposites					1269:1282	TPS/BC/VB nanocomposites	1259:1282	TPS/BC/VB nanocomposites	1259:1282	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	4	18	theme	high-resolution	759:773	arg1	microscopy					779:788	high-resolution SEM microscopy	759:788	high-resolution SEM microscopy	759:788	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	1	19	theme	nanocomposite	194:206	arg1	material					208:215	a nanocomposite material	192:215	a nanocomposite material	192:215	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	6	20	theme	VB	1203:1204	arg1	release					1206:1212	lower VB release	1197:1212	lower VB release	1197:1212	Furthermore, the release of VB was controlled by the BC content, with higher BC content leading to lower VB release.
37148934	0	21	theme	Thermoplastic	0:12	arg1	clay					31:34	Thermoplastic starch/bentonite clay	0:34	Thermoplastic starch/bentonite clay	0:34	Thermoplastic starch/bentonite clay nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior.
37148934	1	22	theme	material	208:215	arg1	characterization					172:187	characterization	172:187	characterization	172:187	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	1	22	theme	material	208:215	arg1	development					156:166	development	156:166	development	156:166	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	0	23	theme	starch/bentonite	14:29	arg1	clay					31:34	Thermoplastic starch/bentonite clay	0:34	Thermoplastic starch/bentonite clay	0:34	Thermoplastic starch/bentonite clay nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior.
37148934	6	24	theme	VB	1126:1127	arg1	release					1115:1121	the release	1111:1121	the release of VB	1111:1127	Furthermore, the release of VB was controlled by the BC content, with higher BC content leading to lower VB release.
37148934	6	25	theme	lower	1197:1201	arg1	release					1206:1212	lower VB release	1197:1212	lower VB release	1197:1212	Furthermore, the release of VB was controlled by the BC content, with higher BC content leading to lower VB release.
37148934	7	26	with	materials	1312:1320	arg1	properties					1347:1356	improved mechanical properties	1327:1356	improved mechanical properties	1327:1356	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	7	26	with	materials	1312:1320	arg1	release					1373:1379	controlled release	1362:1379	controlled release of VB, which can have significant applications in the biopolymer industry	1362:1453	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	3	27	theme	mechanical	565:574	arg1	properties					588:597	mechanical and thermal properties	565:597	mechanical and thermal properties	565:597	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	7	28	theme	nanocomposites	1269:1282	arg1	potential					1246:1254	the potential	1242:1254	the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry	1242:1453	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	2	29	theme	petroleum-based	432:446	arg1	materials					448:456	petroleum-based materials	432:456	petroleum-based materials	432:456	The research is motivated by the potential of TPS as a renewable and biodegradable substitute for petroleum-based materials in the biopolymer industry.
37148934	7	30	theme	significant	1403:1413	arg1	applications					1415:1426	significant applications	1403:1426	significant applications	1403:1426	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	3	31	from	effects	490:496	arg1	properties					527:536	the physicochemical properties	507:536	the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water,	507:638	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	5	32	theme	TPS/BC	992:997	arg1	films					999:1003	TPS/BC films	992:1003	TPS/BC films	992:1003	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	7	33	theme	mechanical	1336:1345	arg1	properties					1347:1356	improved mechanical properties	1327:1356	improved mechanical properties	1327:1356	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	4	34	theme	samples	731:737	arg1	morphology					684:693	surface morphology	676:693	surface morphology	676:693	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	4	34	theme	samples	731:737	arg1	composition					708:718	chemical composition	699:718	chemical composition	699:718	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	3	35	from	properties	588:597	arg1	water					633:637	water	633:637	water	633:637	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	4	36	theme	TPS	727:729	arg1	samples					731:737	the TPS samples	723:737	the TPS samples	723:737	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	1	37	theme	thermoplastic	232:244	arg1	TPS					254:256	TPS	254:256	TPS	254:256	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	1	37	theme	thermoplastic	232:244	arg1	starch					246:251	thermoplastic starch	232:251	thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB)	232:331	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	5	38	contain	containing	1058:1067	arg1	nanocomposites					1043:1056	nanocomposites	1043:1056	nanocomposites containing 5 php of VB and 3 php of BC	1043:1095	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	5	38	contain	containing	1058:1067	arg2	php					1071:1073	5 php	1069:1073	5 php of VB	1069:1079	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	5	38	contain	containing	1058:1067	arg2	php					1087:1089	3 php	1085:1089	3 php of BC	1085:1095	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	6	39	theme	BC	1175:1176	arg1	content					1178:1184	higher BC content	1168:1184	higher BC content leading to lower VB release	1168:1212	Furthermore, the release of VB was controlled by the BC content, with higher BC content leading to lower VB release.
37148934	2	40	dep	renewable	389:397	arg1	substitute					417:426	substitute	417:426	substitute for petroleum-based materials in the biopolymer industry	417:483	The research is motivated by the potential of TPS as a renewable and biodegradable substitute for petroleum-based materials in the biopolymer industry.
37148934	2	41	theme	biopolymer	465:474	arg1	industry					476:483	the biopolymer industry	461:483	the biopolymer industry	461:483	The research is motivated by the potential of TPS as a renewable and biodegradable substitute for petroleum-based materials in the biopolymer industry.
37148934	1	42	theme	vitamin	317:323	arg1	B2					325:326	vitamin B2	317:326	vitamin B2 (VB)	317:331	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	1	42	theme	vitamin	317:323	arg1	VB					329:330	VB	329:330	VB	329:330	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	7	43	theme	environmentally	1287:1301	arg1	materials					1312:1320	environmentally friendly materials	1287:1320	environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry	1287:1453	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	3	44	theme	water	600:604	arg1	uptake					606:611	water uptake	600:611	water uptake	600:611	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	0	45	theme	B2	74:75	arg1	characteristics					94:108	vitamin B2: Physicochemical characteristics	66:108	vitamin B2: Physicochemical characteristics	66:108	Thermoplastic starch/bentonite clay nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior.
37148934	7	46	contain	have	1398:1401	arg2	applications					1415:1426	significant applications	1403:1426	significant applications	1403:1426	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	7	46	contain	have	1398:1401	arg1	VB					1384:1385	VB	1384:1385	VB	1384:1385	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	7	47	theme	improved	1327:1334	arg1	properties					1347:1356	improved mechanical properties	1327:1356	improved mechanical properties	1327:1356	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	5	48	theme	VB	1078:1079	arg1	php					1087:1089	3 php	1085:1089	3 php of BC	1085:1095	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	5	48	theme	VB	1078:1079	arg1	php					1071:1073	5 php	1069:1073	5 php of VB	1069:1079	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	3	49	theme	thermal	580:586	arg1	properties					588:597	mechanical and thermal properties	565:597	mechanical and thermal properties	565:597	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	0	50	theme	vitamin	66:72	arg1	characteristics					94:108	vitamin B2: Physicochemical characteristics	66:108	vitamin B2: Physicochemical characteristics	66:108	Thermoplastic starch/bentonite clay nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior.
37148934	7	51	theme	controlled	1362:1371	arg1	release					1373:1379	controlled release	1362:1379	controlled release of VB, which can have significant applications in the biopolymer industry	1362:1453	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	6	52	theme	higher	1168:1173	arg1	content					1178:1184	higher BC content	1168:1184	higher BC content leading to lower VB release	1168:1212	Furthermore, the release of VB was controlled by the BC content, with higher BC content leading to lower VB release.
37148934	7	53	theme	friendly	1303:1310	arg1	materials					1312:1320	environmentally friendly materials	1287:1320	environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry	1287:1453	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	0	54	dep	nanocomposite	36:48	arg1	reinforced					50:59	reinforced	50:59	nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior	36:129	Thermoplastic starch/bentonite clay nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior.
37148934	1	55	dep	starch	246:251	arg1	reinforced					259:268	reinforced	259:268	reinforced with bentonite clay (BC)	259:293	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	1	55	dep	starch	246:251	arg1	encapsulated					299:310	encapsulated	299:310	encapsulated with vitamin B2 (VB)	299:331	This study presents the development and characterization of a nanocomposite material, consisting of thermoplastic starch (TPS) reinforced with bentonite clay (BC) and encapsulated with vitamin B2 (VB).
37148934	5	56	theme	VB	921:922	arg1	addition					909:916	the addition	905:916	the addition of VB	905:922	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	5	57	theme	tensile	952:958	arg1	strength					960:967	tensile strength	952:967	tensile strength	952:967	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	7	58	theme	VB	1384:1385	arg1	properties					1347:1356	improved mechanical properties	1327:1356	improved mechanical properties	1327:1356	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	7	58	theme	VB	1384:1385	arg1	release					1373:1379	controlled release	1362:1379	controlled release of VB, which can have significant applications in the biopolymer industry	1362:1453	These findings demonstrate the potential of TPS/BC/VB nanocomposites as environmentally friendly materials with improved mechanical properties and controlled release of VB, which can have significant applications in the biopolymer industry.
37148934	3	59	theme	weight	618:623	arg1	loss					625:628	weight loss	618:628	weight loss in water	618:637	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
37148934	4	60	theme	chemical	699:706	arg1	composition					708:718	chemical composition	699:718	chemical composition	699:718	In addition, the surface morphology and chemical composition of the TPS samples were analyzed using high-resolution SEM microscopy and EDS, providing insight into the structure-property relationship of the nanocomposites.
37148934	0	61	theme	Physicochemical	78:92	arg1	characteristics					94:108	vitamin B2: Physicochemical characteristics	66:108	vitamin B2: Physicochemical characteristics	66:108	Thermoplastic starch/bentonite clay nanocomposite reinforced with vitamin B2: Physicochemical characteristics and release behavior.
37148934	5	62	theme	films	999:1003	arg1	strength					960:967	tensile strength	952:967	tensile strength	952:967	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	5	62	theme	films	999:1003	arg1	modulus					981:987	Young's modulus	973:987	Young's modulus	973:987	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	2	63	theme	TPS	380:382	arg1	potential					367:375	the potential	363:375	the potential of TPS as a renewable and biodegradable substitute for petroleum-based materials in the biopolymer industry	363:483	The research is motivated by the potential of TPS as a renewable and biodegradable substitute for petroleum-based materials in the biopolymer industry.
37148934	5	64	theme	BC	1094:1095	arg1	php					1087:1089	3 php	1085:1089	3 php of BC	1085:1095	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	5	64	theme	BC	1094:1095	arg1	php					1071:1073	5 php	1069:1073	5 php of VB	1069:1079	The results showed that the addition of VB significantly increased the tensile strength and Young's modulus of TPS/BC films, with the highest values observed for nanocomposites containing 5 php of VB and 3 php of BC.
37148934	6	65	theme	BC	1151:1152	arg1	content					1154:1160	the BC content	1147:1160	the BC content	1147:1160	Furthermore, the release of VB was controlled by the BC content, with higher BC content leading to lower VB release.
37148934	3	66	theme	physicochemical	511:525	arg1	properties					527:536	the physicochemical properties	507:536	the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water,	507:638	The effects of VB on the physicochemical properties of TPS/BC films, including mechanical and thermal properties, water uptake, and weight loss in water, were investigated.
36076432	6	0	theme	ether	1141:1145	arg1	glucuronide					1147:1157	ellagic acid dimethyl ether glucuronide	1119:1157	ellagic acid dimethyl ether glucuronide	1119:1157	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	1	1	from	effects	252:258	arg1	function					292:299	liver function	286:299	liver function	286:299	How stimulation of the intestinal microbiota may regulate the health-promoting effects of dietary polyphenols on liver function has not been fully elucidated.
36076432	10	2	theme	antioxidative	1948:1960	arg1	status					1962:1967	antioxidative status	1948:1967	antioxidative status	1948:1967	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	4	3	with	diet	750:753	arg1	PP					764:765	the PP	760:765	the PP (CPF group)	760:777	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	4	3	with	diet	750:753	arg1	group					772:776	CPF group	768:776	CPF group	768:776	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	1	4	theme	polyphenols	271:281	arg1	effects					252:258	the health-promoting effects	231:258	the health-promoting effects of dietary polyphenols on liver function	231:299	How stimulation of the intestinal microbiota may regulate the health-promoting effects of dietary polyphenols on liver function has not been fully elucidated.
36076432	10	5	theme	metabolite	1882:1891	arg1	levels					1893:1898	polyphenolic metabolite levels	1869:1898	polyphenolic metabolite levels in the liver	1869:1911	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	4	6	theme	propionic	904:912	arg1	acid					914:917	propionic acid	904:917	propionic acid	904:917	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	4	6	theme	propionic	904:912	arg1	acids					890:894	short-chain fatty acids	872:894	short-chain fatty acids	872:894	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	11	7	theme	valuable	2048:2055	arg1	supplement					2065:2074	a valuable dietary supplement	2046:2074	a valuable dietary supplement that is able to support preventive healthcare	2046:2120	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	7	theme	valuable	2048:2055	arg1	able					2084:2087	able	2084:2087	able	2084:2087	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	7	theme	valuable	2048:2055	arg1	combination					2001:2011	the combination	1997:2011	the combination of FOSs and polyphenols	1997:2035	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	1	8	theme	liver	286:290	arg1	function					292:299	liver function	286:299	liver function	286:299	How stimulation of the intestinal microbiota may regulate the health-promoting effects of dietary polyphenols on liver function has not been fully elucidated.
36076432	9	9	theme	dismutase	1695:1703	arg1	activity					1672:1679	activity	1672:1679	activity	1672:1679	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	9	9	theme	dismutase	1695:1703	arg1	status					1661:1666	total antioxidant status	1643:1666	total antioxidant status	1643:1666	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	2	10	link	polyphenol-derived	529:546	arg1	compounds					558:566	polyphenol-derived bioactive compounds	529:566	polyphenol-derived bioactive compounds	529:566	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	10	11	theme	healthy	1972:1978	arg1	rats					1980:1983	healthy rats	1972:1983	healthy rats	1972:1983	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	10	12	theme	microbial	1762:1770	arg1	composition					1772:1782	the intestinal microbial composition	1747:1782	the intestinal microbial composition	1747:1782	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	4	13	theme	fatty	884:888	arg1	acid					914:917	propionic acid	904:917	propionic acid	904:917	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	4	13	theme	fatty	884:888	arg1	acids					890:894	short-chain fatty acids	872:894	short-chain fatty acids	872:894	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	10	14	from	status	1962:1967	arg1	rats					1980:1983	healthy rats	1972:1983	healthy rats	1972:1983	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	0	15	theme	metabolism	122:131	arg1	levels					74:79	hepatic levels	66:79	hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status	66:154	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	6	16	theme	dimethyl	1132:1139	arg1	glucuronide					1147:1157	ellagic acid dimethyl ether glucuronide	1119:1157	ellagic acid dimethyl ether glucuronide	1119:1157	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	1	17	theme	microbiota	207:216	arg1	stimulation					177:187	stimulation	177:187	stimulation of the intestinal microbiota	177:216	How stimulation of the intestinal microbiota may regulate the health-promoting effects of dietary polyphenols on liver function has not been fully elucidated.
36076432	2	18	theme	bioactive	548:556	arg1	compounds					558:566	polyphenol-derived bioactive compounds	529:566	polyphenol-derived bioactive compounds	529:566	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	8	19	theme	reduced	1481:1487	arg1	levels					1489:1494	the considerably reduced levels	1464:1494	the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group	1464:1569	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	7	20	theme	lipid	1363:1367	arg1	metabolism					1369:1378	lipid metabolism	1363:1378	lipid metabolism	1363:1378	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	0	21	theme	antioxidant	137:147	arg1	status					149:154	antioxidant status	137:154	antioxidant status	137:154	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	3	22	theme	lipid	624:628	arg1	metabolism					630:639	lipid metabolism	624:639	lipid metabolism	624:639	We also investigated how this supplementation affected lipid metabolism and antioxidative status in Wistar rats without diet-induced metabolic disturbances.
36076432	10	23	theme	health-promoting	1821:1836	arg1	effects					1838:1844	the health-promoting effects	1817:1844	the health-promoting effects of the PP	1817:1854	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	9	24	from	activity	1672:1679	arg1	blood					1712:1716	the blood	1708:1716	the blood	1708:1716	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	10	25	theme	polyphenolic	1869:1880	arg1	levels					1893:1898	polyphenolic metabolite levels	1869:1898	polyphenolic metabolite levels in the liver	1869:1911	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	9	26	theme	FOSs	1601:1604	arg1	combination					1586:1596	the combination	1582:1596	the combination of FOSs and the PP	1582:1615	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	11	27	theme	polyphenols	2025:2035	arg1	supplement					2065:2074	a valuable dietary supplement	2046:2074	a valuable dietary supplement that is able to support preventive healthcare	2046:2120	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	27	theme	polyphenols	2025:2035	arg1	able					2084:2087	able	2084:2087	able	2084:2087	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	27	theme	polyphenols	2025:2035	arg1	combination					2001:2011	the combination	1997:2011	the combination of FOSs and polyphenols	1997:2035	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	5	28	theme	relative	954:961	arg1	abundances					963:972	increased relative abundances	944:972	increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation	944:1062	The CPF group exhibited increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation.
36076432	7	29	theme	antioxidative	1384:1396	arg1	status					1398:1403	antioxidative status	1384:1403	antioxidative status	1384:1403	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	0	30	theme	hepatic	66:72	arg1	levels					74:79	hepatic levels	66:79	hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status	66:154	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	10	31	theme	PP	1853:1854	arg1	effects					1838:1844	the health-promoting effects	1817:1844	the health-promoting effects of the PP	1817:1854	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	2	32	theme	intestinal	479:488	arg1	microbiota					490:499	the intestinal microbiota	475:499	the intestinal microbiota	475:499	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	8	33	theme	peroxidation	1528:1539	arg1	levels					1489:1494	the considerably reduced levels	1464:1494	the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group	1464:1569	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	5	34	theme	CPF	924:926	arg1	group					928:932	The CPF group	920:932	The CPF group	920:932	The CPF group exhibited increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation.
36076432	0	35	theme	polyphenolic	92:103	arg1	compounds					105:113	dietary polyphenolic compounds	84:113	dietary polyphenolic compounds	84:113	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	2	36	theme	liver	513:517	arg1	levels					519:524	liver levels	513:524	liver levels of polyphenol-derived bioactive compounds	513:566	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	2	37	theme	fructooligosaccharides	395:416	arg1	combination					380:390	a combination	378:390	a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP)	378:457	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	6	38	theme	ellagic	1119:1125	arg1	acid					1127:1130	ellagic acid	1119:1130	ellagic acid dimethyl ether glucuronide	1119:1157	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	8	39	theme	CPF	1561:1563	arg1	group					1565:1569	the CPF group	1557:1569	the CPF group	1557:1569	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	3	40	from	status	659:664	arg1	rats					676:679	Wistar rats	669:679	Wistar rats	669:679	We also investigated how this supplementation affected lipid metabolism and antioxidative status in Wistar rats without diet-induced metabolic disturbances.
36076432	6	41	theme	acid	1113:1116	arg1	concentrations					1087:1100	Considerably elevated concentrations	1065:1100	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside	1065:1214	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	2	42	theme	polyphenol	431:440	arg1	preparation					442:452	a polyphenol preparation	429:452	a polyphenol preparation (PP)	429:457	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	2	42	theme	polyphenol	431:440	arg1	PP					455:456	PP	455:456	PP	455:456	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	5	43	theme	bacteria	984:991	arg1	abundances					963:972	increased relative abundances	944:972	increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation	944:1062	The CPF group exhibited increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation.
36076432	0	44	theme	microbiota	30:39	arg1	Stimulation					0:10	Stimulation	0:10	Stimulation of the intestinal microbiota with prebiotics	0:55	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	9	45	theme	PP	1614:1615	arg1	combination					1586:1596	the combination	1582:1596	the combination of FOSs and the PP	1582:1615	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	7	46	theme	molecular	1331:1339	arg1	factors					1341:1347	molecular factors	1331:1347	molecular factors that regulate lipid metabolism and antioxidative status	1331:1403	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	7	46	theme	molecular	1331:1339	arg1	SREBP1c					1312:1318	SREBP1c	1312:1318	SREBP1c	1312:1318	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	7	46	theme	molecular	1331:1339	arg1	AHR					1304:1306	AHR	1304:1306	AHR	1304:1306	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	7	46	theme	molecular	1331:1339	arg1	HIF1α					1297:1301	HIF1α	1297:1301	HIF1α	1297:1301	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	3	47	theme	Wistar	669:674	arg1	rats					676:679	Wistar rats	669:679	Wistar rats	669:679	We also investigated how this supplementation affected lipid metabolism and antioxidative status in Wistar rats without diet-induced metabolic disturbances.
36076432	11	48	theme	FOSs	2016:2019	arg1	supplement					2065:2074	a valuable dietary supplement	2046:2074	a valuable dietary supplement that is able to support preventive healthcare	2046:2120	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	48	theme	FOSs	2016:2019	arg1	able					2084:2087	able	2084:2087	able	2084:2087	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	48	theme	FOSs	2016:2019	arg1	combination					2001:2011	the combination	1997:2011	the combination of FOSs and polyphenols	1997:2035	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	0	49	with	Stimulation	0:10	arg1	prebiotics					46:55	prebiotics	46:55	prebiotics	46:55	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	9	50	theme	total	1643:1647	arg1	status					1661:1666	total antioxidant status	1643:1666	total antioxidant status	1643:1666	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	7	51	theme	SREBP1c	1312:1318	arg1	downregulation					1279:1292	hepatic downregulation	1271:1292	hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status,	1271:1404	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	6	52	theme	elevated	1078:1085	arg1	concentrations					1087:1100	Considerably elevated concentrations	1065:1100	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside	1065:1214	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	4	53	theme	microbial	823:831	arg1	enzymes					833:839	microbial enzymes	823:839	microbial enzymes	823:839	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	10	54	theme	intestinal	1751:1760	arg1	composition					1772:1782	the intestinal microbial composition	1747:1782	the intestinal microbial composition	1747:1782	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	7	55	theme	AHR	1304:1306	arg1	downregulation					1279:1292	hepatic downregulation	1271:1292	hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status,	1271:1404	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	8	56	from	levels	1489:1494	arg1	liver					1548:1552	the liver	1544:1552	the liver in the CPF group	1544:1569	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	1	57	theme	dietary	263:269	arg1	polyphenols					271:281	dietary polyphenols	263:281	dietary polyphenols	263:281	How stimulation of the intestinal microbiota may regulate the health-promoting effects of dietary polyphenols on liver function has not been fully elucidated.
36076432	4	58	theme	acids	890:894	arg1	production					858:867	the production	854:867	the production of short-chain fatty acids, mostly propionic acid	854:917	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	7	59	theme	HIF1α	1297:1301	arg1	downregulation					1279:1292	hepatic downregulation	1271:1292	hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status,	1271:1404	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	10	60	theme	activity	1788:1795	arg1	stimulation					1732:1742	stimulation	1732:1742	stimulation of the intestinal microbial composition and activity with FOSs	1732:1805	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	7	61	located	found	1415:1419	arg2	downregulation					1279:1292	hepatic downregulation	1271:1292	hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status,	1271:1404	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	7	61	located	found	1415:1419	arg1	group					1429:1433	this group	1424:1433	this group	1424:1433	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	7	61	located	found	1415:1419	arg1	addition					1261:1268	addition	1261:1268	addition	1261:1268	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	9	62	theme	superoxide	1684:1693	arg1	dismutase					1695:1703	superoxide dismutase	1684:1703	superoxide dismutase	1684:1703	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	10	63	theme	composition	1772:1782	arg1	stimulation					1732:1742	stimulation	1732:1742	stimulation of the intestinal microbial composition and activity with FOSs	1732:1805	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	10	64	from	metabolism	1933:1942	arg1	rats					1980:1983	healthy rats	1972:1983	healthy rats	1972:1983	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	4	65	theme	short-chain	872:882	arg1	acid					914:917	propionic acid	904:917	propionic acid	904:917	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	4	65	theme	short-chain	872:882	arg1	acids					890:894	short-chain fatty acids	872:894	short-chain fatty acids	872:894	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	11	66	theme	dietary	2057:2063	arg1	supplement					2065:2074	a valuable dietary supplement	2046:2074	a valuable dietary supplement that is able to support preventive healthcare	2046:2120	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	66	theme	dietary	2057:2063	arg1	able					2084:2087	able	2084:2087	able	2084:2087	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	11	66	theme	dietary	2057:2063	arg1	combination					2001:2011	the combination	1997:2011	the combination of FOSs and polyphenols	1997:2035	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	1	67	theme	intestinal	196:205	arg1	microbiota					207:216	the intestinal microbiota	192:216	the intestinal microbiota	192:216	How stimulation of the intestinal microbiota may regulate the health-promoting effects of dietary polyphenols on liver function has not been fully elucidated.
36076432	2	68	theme	compounds	558:566	arg1	levels					519:524	liver levels	513:524	liver levels of polyphenol-derived bioactive compounds	513:566	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	6	69	theme	cyanidin-3-sophoroside	1193:1214	arg1	concentrations					1087:1100	Considerably elevated concentrations	1065:1100	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside	1065:1214	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	9	70	dep	status	1661:1666	arg1	the					1639:1641	the	1639:1641	the	1639:1641	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	0	71	theme	status	149:154	arg1	levels					74:79	hepatic levels	66:79	hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status	66:154	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	5	72	from	preparation	1052:1062	arg1	polyphenols					1022:1032	polyphenols	1022:1032	polyphenols from the examined preparation	1022:1062	The CPF group exhibited increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation.
36076432	2	73	theme	polyphenol-derived	529:546	arg1	compounds					558:566	polyphenol-derived bioactive compounds	529:566	polyphenol-derived bioactive compounds	529:566	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	8	74	theme	fat	1499:1501	arg1	levels					1489:1494	the considerably reduced levels	1464:1494	the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group	1464:1569	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	0	75	theme	healthy	159:165	arg1	rats					167:170	healthy rats	159:170	healthy rats	159:170	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	8	76	theme	triglycerides	1504:1516	arg1	levels					1489:1494	the considerably reduced levels	1464:1494	the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group	1464:1569	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	3	77	theme	antioxidative	645:657	arg1	status					659:664	antioxidative status	645:664	antioxidative status	645:664	We also investigated how this supplementation affected lipid metabolism and antioxidative status in Wistar rats without diet-induced metabolic disturbances.
36076432	5	78	theme	increased	944:952	arg1	abundances					963:972	increased relative abundances	944:972	increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation	944:1062	The CPF group exhibited increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation.
36076432	8	79	theme	lipid	1522:1526	arg1	peroxidation					1528:1539	lipid peroxidation	1522:1539	lipid peroxidation	1522:1539	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	7	80	theme	hepatic	1271:1277	arg1	downregulation					1279:1292	hepatic downregulation	1271:1292	hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status,	1271:1404	In addition, hepatic downregulation of HIF1α, AHR and SREBP1c, which are molecular factors that regulate lipid metabolism and antioxidative status, was also found in this group.
36076432	6	81	theme	CPF	1247:1249	arg1	group					1251:1255	the CPF group	1243:1255	the CPF group	1243:1255	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	0	82	theme	dietary	84:90	arg1	compounds					105:113	dietary polyphenolic compounds	84:113	dietary polyphenolic compounds	84:113	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	11	83	theme	preventive	2100:2109	arg1	healthcare					2111:2120	preventive healthcare	2100:2120	preventive healthcare	2100:2120	Therefore, the combination of FOSs and polyphenols might be a valuable dietary supplement that is able to support preventive healthcare.
36076432	4	84	theme	FOSs	738:741	arg1	Addition					726:733	Addition	726:733	Addition of FOSs to the diet with the PP (CPF group)	726:777	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	0	85	theme	compounds	105:113	arg1	levels					74:79	hepatic levels	66:79	hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status	66:154	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	3	86	from	metabolism	630:639	arg1	rats					676:679	Wistar rats	669:679	Wistar rats	669:679	We also investigated how this supplementation affected lipid metabolism and antioxidative status in Wistar rats without diet-induced metabolic disturbances.
36076432	6	87	theme	4-hydroxybenzoic	1160:1175	arg1	acid					1177:1180	4-hydroxybenzoic acid	1160:1180	4-hydroxybenzoic acid	1160:1180	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	0	88	theme	lipid	116:120	arg1	metabolism					122:131	lipid metabolism	116:131	lipid metabolism	116:131	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	9	89	from	status	1661:1666	arg1	blood					1712:1716	the blood	1708:1716	the blood	1708:1716	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
36076432	6	90	theme	acid	1127:1130	arg1	glucuronide					1147:1157	ellagic acid dimethyl ether glucuronide	1119:1157	ellagic acid dimethyl ether glucuronide	1119:1157	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	8	91	from	liver	1548:1552	arg1	group					1565:1569	the CPF group	1557:1569	the CPF group	1557:1569	These changes might explain the considerably reduced levels of fat, triglycerides and lipid peroxidation in the liver in the CPF group.
36076432	2	92	theme	preparation	442:452	arg1	combination					380:390	a combination	378:390	a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP)	378:457	Therefore, in this study, we examined whether a combination of fructooligosaccharides (FOSs) and a polyphenol preparation (PP) might stimulate the intestinal microbiota to increase liver levels of polyphenol-derived bioactive compounds.
36076432	3	93	theme	metabolic	702:710	arg1	disturbances					712:723	diet-induced metabolic disturbances	689:723	diet-induced metabolic disturbances	689:723	We also investigated how this supplementation affected lipid metabolism and antioxidative status in Wistar rats without diet-induced metabolic disturbances.
36076432	0	94	theme	intestinal	19:28	arg1	microbiota					30:39	the intestinal microbiota	15:39	the intestinal microbiota	15:39	Stimulation of the intestinal microbiota with prebiotics enhances hepatic levels of dietary polyphenolic compounds, lipid metabolism and antioxidant status in healthy rats.
36076432	5	95	theme	caecal	977:982	arg1	bacteria					984:991	caecal bacteria	977:991	caecal bacteria	977:991	The CPF group exhibited increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation.
36076432	6	96	theme	ellagic	1105:1111	arg1	acid					1113:1116	ellagic acid	1105:1116	ellagic acid	1105:1116	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	4	97	theme	CPF	768:770	arg1	PP					764:765	the PP	760:765	the PP (CPF group)	760:777	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	4	97	theme	CPF	768:770	arg1	group					772:776	CPF group	768:776	CPF group	768:776	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	5	98	theme	examined	1043:1050	arg1	preparation					1052:1062	the examined preparation	1039:1062	the examined preparation	1039:1062	The CPF group exhibited increased relative abundances of caecal bacteria that were able to metabolize polyphenols from the examined preparation.
36076432	4	99	theme	enzymes	833:839	arg1	activity					811:818	the caecal activity	800:818	the caecal activity of microbial enzymes	800:839	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	6	100	theme	methyl	1186:1191	arg1	cyanidin-3-sophoroside					1193:1214	methyl cyanidin-3-sophoroside	1186:1214	methyl cyanidin-3-sophoroside	1186:1214	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	10	101	theme	lipid	1927:1931	arg1	metabolism					1933:1942	lipid metabolism	1927:1942	lipid metabolism	1927:1942	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	10	102	with	stimulation	1732:1742	arg1	FOSs					1802:1805	FOSs	1802:1805	FOSs	1802:1805	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	6	103	theme	acid	1177:1180	arg1	concentrations					1087:1100	Considerably elevated concentrations	1065:1100	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside	1065:1214	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	6	104	located	found	1221:1225	arg1	liver					1234:1238	the liver	1230:1238	the liver	1230:1238	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	6	104	located	found	1221:1225	arg1	group					1251:1255	the CPF group	1243:1255	the CPF group	1243:1255	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	6	104	located	found	1221:1225	arg2	concentrations					1087:1100	Considerably elevated concentrations	1065:1100	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside	1065:1214	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	3	105	theme	diet-induced	689:700	arg1	disturbances					712:723	diet-induced metabolic disturbances	689:723	diet-induced metabolic disturbances	689:723	We also investigated how this supplementation affected lipid metabolism and antioxidative status in Wistar rats without diet-induced metabolic disturbances.
36076432	4	106	theme	caecal	804:809	arg1	activity					811:818	the caecal activity	800:818	the caecal activity of microbial enzymes	800:839	Addition of FOSs to the diet with the PP (CPF group) considerably reduced the caecal activity of microbial enzymes and elevated the production of short-chain fatty acids, mostly propionic acid.
36076432	6	107	theme	glucuronide	1147:1157	arg1	concentrations					1087:1100	Considerably elevated concentrations	1065:1100	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside	1065:1214	Considerably elevated concentrations of ellagic acid, ellagic acid dimethyl ether glucuronide, 4-hydroxybenzoic acid and methyl cyanidin-3-sophoroside were found in the liver in the CPF group.
36076432	1	108	theme	health-promoting	235:250	arg1	effects					252:258	the health-promoting effects	231:258	the health-promoting effects of dietary polyphenols on liver function	231:299	How stimulation of the intestinal microbiota may regulate the health-promoting effects of dietary polyphenols on liver function has not been fully elucidated.
36076432	10	109	from	levels	1893:1898	arg1	liver					1907:1911	the liver	1903:1911	the liver	1903:1911	To conclude, stimulation of the intestinal microbial composition and activity with FOSs increased the health-promoting effects of the PP by elevating polyphenolic metabolite levels in the liver and improving lipid metabolism and antioxidative status in healthy rats.
36076432	9	110	theme	antioxidant	1649:1659	arg1	status					1661:1666	total antioxidant status	1643:1666	total antioxidant status	1643:1666	Moreover, the combination of FOSs and the PP considerably elevated the total antioxidant status and activity of superoxide dismutase in the blood.
35224663	10	0	theme	genes	1631:1635	arg1	Characterization					1574:1589	Characterization	1574:1589	Characterization of these two groups of co-expressed key genes	1574:1635	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	8	1	attach	linked	1273:1278	arg1	content					1289:1295	sugar content	1283:1295	sugar content	1283:1295	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	8	1	attach	linked	1273:1278	arg1	content					1307:1313	fibre content	1301:1313	fibre content	1301:1313	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	8	1	attach	linked	1273:1278	arg2	pathways					1264:1271	interconnected metabolic pathways	1239:1271	interconnected metabolic pathways linked to sugar content and fibre content	1239:1313	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	1	2	theme	bio-energy	248:257	arg1	production					234:243	the production	230:243	the production of bio-energy, bio-plastics and high-value products	230:295	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	6	3	theme	co-expression	907:919	arg1	WGCNA					939:943	WGCNA	939:943	WGCNA	939:943	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	6	3	theme	co-expression	907:919	arg1	analysis					929:936	Weighted gene co-expression network analysis	893:936	Weighted gene co-expression network analysis (WGCNA)	893:944	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	10	4	theme	high	1690:1693	arg1	species					1701:1707	high sugar species	1690:1707	high sugar species	1690:1707	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	8	5	theme	metabolic	1254:1262	arg1	pathways					1264:1271	interconnected metabolic pathways	1239:1271	interconnected metabolic pathways linked to sugar content and fibre content	1239:1313	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	10	6	theme	co-expressed	1614:1625	arg1	genes					1631:1635	key genes	1627:1635	co-expressed key genes	1614:1635	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	0	7	theme	Transcriptome	0:12	arg1	changes					14:20	Transcriptome changes	0:20	Transcriptome changes in the developing sugarcane	0:48	Transcriptome changes in the developing sugarcane culm associated with high yield and early-season high sugar content.
35224663	5	8	theme	development	873:883	arg1	stages					885:890	five different development stages	858:890	five different development stages	858:890	In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 120 samples in triplicate across 24 sugarcane cultivars at five different development stages.
35224663	8	9	theme	Correlation	1189:1199	arg1	analysis					1201:1208	Correlation analysis	1189:1208	Correlation analysis	1189:1208	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	6	10	theme	Weighted	893:900	arg1	WGCNA					939:943	WGCNA	939:943	WGCNA	939:943	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	6	10	theme	Weighted	893:900	arg1	analysis					929:936	Weighted gene co-expression network analysis	893:936	Weighted gene co-expression network analysis (WGCNA)	893:944	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	7	11	theme	Gene	1092:1095	arg1	identification					1097:1110	Gene identification	1092:1110	Gene identification	1092:1110	Gene identification and metabolism pathways analysis was used to define these two sets of genes.
35224663	9	12	theme	dominant	1487:1494	arg1	influence					1504:1512	a dominant genetic influence	1485:1512	a dominant genetic influence on biomass composition	1485:1535	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	6	13	theme	first	976:980	arg1	time					982:985	the first time	972:985	the first time	972:985	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	8	14	theme	sugar	1283:1287	arg1	content					1289:1295	sugar content	1283:1295	sugar content	1283:1295	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	6	15	used	used	950:953	arg2	WGCNA					939:943	WGCNA	939:943	WGCNA	939:943	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	6	15	used	used	950:953	arg2	analysis					929:936	Weighted gene co-expression network analysis	893:936	Weighted gene co-expression network analysis (WGCNA)	893:944	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	8	16	theme	large	1223:1227	arg1	number					1229:1234	a large number	1221:1234	a large number of interconnected metabolic pathways linked to sugar content and fibre content	1221:1313	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	9	17	theme	biomass	1517:1523	arg1	composition					1525:1535	biomass composition	1517:1535	biomass composition	1517:1535	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	10	18	dep	fibre	1683:1687	arg1	biology					1728:1734	synthetic biology	1718:1734	synthetic biology	1718:1734	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	5	19	from	stages	885:890	arg1	cultivars					845:853	24 sugarcane cultivars	832:853	24 sugarcane cultivars at five different development stages	832:890	In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 120 samples in triplicate across 24 sugarcane cultivars at five different development stages.
35224663	9	20	theme	breeding	1554:1561	arg1	selection					1563:1571	breeding selection	1554:1571	breeding selection	1554:1571	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	1	21	theme	sugar	192:196	arg1	yield					198:202	biomass and sugar yield	180:202	yield	198:202	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	21	theme	sugar	192:196	arg1	assimilation					166:177	its exceptional carbon dioxide assimilation	135:177	its exceptional carbon dioxide assimilation	135:177	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	22	theme	exceptional	139:149	arg1	yield					198:202	biomass and sugar yield	180:202	yield	198:202	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	22	theme	exceptional	139:149	arg1	assimilation					166:177	its exceptional carbon dioxide assimilation	135:177	its exceptional carbon dioxide assimilation	135:177	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	6	23	theme	genes	1012:1016	arg1	sets					991:994	two sets	987:994	two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content	987:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	6	23	theme	genes	1012:1016	arg1	genes					1012:1016	co-expressed genes	999:1016	co-expressed genes with a distinct and opposite correlation between fibre and sugar content	999:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	2	24	theme	sequestration	495:507	arg1	production					450:459	the production	446:459	the production of biomass composition and carbon sequestration	446:507	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	1	25	theme	dioxide	158:164	arg1	yield					198:202	biomass and sugar yield	180:202	yield	198:202	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	25	theme	dioxide	158:164	arg1	assimilation					166:177	its exceptional carbon dioxide assimilation	135:177	its exceptional carbon dioxide assimilation	135:177	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	9	26	dep	revealed	1372:1379	arg1	facilitating					1541:1552	facilitating	1541:1552	facilitating breeding selection	1541:1571	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	9	26	dep	revealed	1372:1379	arg1	suggesting					1474:1483	suggesting	1474:1483	suggesting a dominant genetic influence on biomass composition	1474:1535	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	0	27	theme	early-season	86:97	arg1	content					110:116	early-season high sugar content	86:116	early-season high sugar content	86:116	Transcriptome changes in the developing sugarcane culm associated with high yield and early-season high sugar content.
35224663	9	28	theme	hierarchical	1329:1340	arg1	clustering					1342:1351	Unsupervised hierarchical clustering	1316:1351	Unsupervised hierarchical clustering of gene expression	1316:1370	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	0	29	theme	sugar	104:108	arg1	content					110:116	early-season high sugar content	86:116	early-season high sugar content	86:116	Transcriptome changes in the developing sugarcane culm associated with high yield and early-season high sugar content.
35224663	6	30	theme	sugar	1077:1081	arg1	content					1083:1089	fibre and sugar content	1067:1089	content	1083:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	9	31	theme	stronger	1383:1390	arg1	level					1392:1396	a stronger level	1381:1396	a stronger level of segregation associated with the genotypes than the stage of development	1381:1471	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	2	32	theme	economic	374:381	arg1	viability					383:391	long-term economic viability	364:391	long-term economic viability	364:391	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	7	33	theme	genes	1182:1186	arg1	sets					1174:1177	these two sets	1164:1177	these two sets of genes	1164:1186	Gene identification and metabolism pathways analysis was used to define these two sets of genes.
35224663	7	33	theme	genes	1182:1186	arg1	genes					1182:1186	genes	1182:1186	genes	1182:1186	Gene identification and metabolism pathways analysis was used to define these two sets of genes.
35224663	9	34	theme	expression	1361:1370	arg1	clustering					1342:1351	Unsupervised hierarchical clustering	1316:1351	Unsupervised hierarchical clustering of gene expression	1316:1370	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	1	35	with	Sugarcane	119:127	arg1	yield					198:202	biomass and sugar yield	180:202	yield	198:202	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	35	with	Sugarcane	119:127	arg1	assimilation					166:177	its exceptional carbon dioxide assimilation	135:177	its exceptional carbon dioxide assimilation	135:177	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	6	36	theme	fibre	1067:1071	arg1	content					1083:1089	fibre and sugar content	1067:1089	content	1083:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	4	37	theme	high	677:680	arg1	yield					682:686	high yield	677:686	high yield	677:686	These varieties are characterized by a high early-season sugar content associated with high yield.
35224663	1	38	theme	products	288:295	arg1	production					234:243	the production	230:243	the production of bio-energy, bio-plastics and high-value products	230:295	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	0	39	theme	developing	29:38	arg1	sugarcane					40:48	the developing sugarcane	25:48	the developing sugarcane	25:48	Transcriptome changes in the developing sugarcane culm associated with high yield and early-season high sugar content.
35224663	2	40	theme	biomass	464:470	arg1	composition					472:482	biomass composition	464:482	biomass composition	464:482	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	5	41	theme	samples	803:809	arg1	set					792:794	a set	790:794	a set of 120 samples	790:809	In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 120 samples in triplicate across 24 sugarcane cultivars at five different development stages.
35224663	5	41	theme	samples	803:809	arg1	samples					803:809	120 samples	799:809	120 samples	799:809	In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 120 samples in triplicate across 24 sugarcane cultivars at five different development stages.
35224663	9	42	theme	segregation	1401:1411	arg1	level					1392:1396	a stronger level	1381:1396	a stronger level of segregation associated with the genotypes than the stage of development	1381:1471	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	7	43	used	used	1149:1152	arg2	pathways					1127:1134	metabolism pathways	1116:1134	metabolism pathways	1116:1134	Gene identification and metabolism pathways analysis was used to define these two sets of genes.
35224663	7	43	used	used	1149:1152	arg2	identification					1097:1110	Gene identification	1092:1110	Gene identification	1092:1110	Gene identification and metabolism pathways analysis was used to define these two sets of genes.
35224663	4	44	theme	sugar	647:651	arg1	content					653:659	a high early-season sugar content	627:659	a high early-season sugar content associated with high yield	627:686	These varieties are characterized by a high early-season sugar content associated with high yield.
35224663	2	45	theme	crucial	342:348	arg1	challenge					350:358	A crucial challenge	340:358	A crucial challenge for long-term economic viability and environmental sustainability	340:424	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	7	46	theme	metabolism	1116:1125	arg1	pathways					1127:1134	metabolism pathways	1116:1134	metabolism pathways	1116:1134	Gene identification and metabolism pathways analysis was used to define these two sets of genes.
35224663	6	47	theme	network	921:927	arg1	WGCNA					939:943	WGCNA	939:943	WGCNA	939:943	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	6	47	theme	network	921:927	arg1	analysis					929:936	Weighted gene co-expression network analysis	893:936	Weighted gene co-expression network analysis (WGCNA)	893:944	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	10	48	theme	key	1627:1629	arg1	genes					1631:1635	key genes	1627:1635	co-expressed key genes	1614:1635	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	5	49	theme	different	863:871	arg1	stages					885:890	five different development stages	858:890	five different development stages	858:890	In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 120 samples in triplicate across 24 sugarcane cultivars at five different development stages.
35224663	6	50	theme	gene	902:905	arg1	WGCNA					939:943	WGCNA	939:943	WGCNA	939:943	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	6	50	theme	gene	902:905	arg1	analysis					929:936	Weighted gene co-expression network analysis	893:936	Weighted gene co-expression network analysis (WGCNA)	893:944	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	8	51	theme	interconnected	1239:1252	arg1	pathways					1264:1271	interconnected metabolic pathways	1239:1271	interconnected metabolic pathways linked to sugar content and fibre content	1239:1313	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	10	52	theme	breeding	1657:1664	arg1	program					1666:1672	breeding program	1657:1672	breeding program for high fibre, high sugar species or plant synthetic biology	1657:1734	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	1	53	theme	biomass	180:186	arg1	yield					198:202	biomass and sugar yield	180:202	yield	198:202	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	53	theme	biomass	180:186	arg1	assimilation					166:177	its exceptional carbon dioxide assimilation	135:177	its exceptional carbon dioxide assimilation	135:177	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	8	54	theme	pathways	1264:1271	arg1	number					1229:1234	a large number	1221:1234	a large number of interconnected metabolic pathways linked to sugar content and fibre content	1221:1313	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	5	55	theme	sugarcane	835:843	arg1	cultivars					845:853	24 sugarcane cultivars	832:853	24 sugarcane cultivars at five different development stages	832:890	In order to investigate these correlations, 1,440 internodes were collected and combined to generate a set of 120 samples in triplicate across 24 sugarcane cultivars at five different development stages.
35224663	4	56	theme	early-season	634:645	arg1	content					653:659	a high early-season sugar content	627:659	a high early-season sugar content associated with high yield	627:686	These varieties are characterized by a high early-season sugar content associated with high yield.
35224663	6	57	with	genes	1012:1016	arg1	correlation					1047:1057	a distinct and opposite correlation	1023:1057	a distinct and opposite correlation between fibre and sugar content	1023:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	7	58	dep	identification	1097:1110	arg1	analysis					1136:1143	analysis	1136:1143	analysis	1136:1143	Gene identification and metabolism pathways analysis was used to define these two sets of genes.
35224663	8	59	theme	fibre	1301:1305	arg1	content					1307:1313	fibre content	1301:1313	fibre content	1301:1313	Correlation analysis identified a large number of interconnected metabolic pathways linked to sugar content and fibre content.
35224663	6	60	theme	distinct	1025:1032	arg1	correlation					1047:1057	a distinct and opposite correlation	1023:1057	a distinct and opposite correlation between fibre and sugar content	1023:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	0	61	theme	high	71:74	arg1	yield					76:80	high yield	71:80	high yield	71:80	Transcriptome changes in the developing sugarcane culm associated with high yield and early-season high sugar content.
35224663	9	62	theme	genetic	1496:1502	arg1	influence					1504:1512	a dominant genetic influence	1485:1512	a dominant genetic influence on biomass composition	1485:1535	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	1	63	theme	pharmaceutical	313:326	arg1	industries					328:337	the food and pharmaceutical industries	300:337	the food and pharmaceutical industries	300:337	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	64	theme	carbon	151:156	arg1	yield					198:202	biomass and sugar yield	180:202	yield	198:202	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	64	theme	carbon	151:156	arg1	assimilation					166:177	its exceptional carbon dioxide assimilation	135:177	its exceptional carbon dioxide assimilation	135:177	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	6	65	theme	co-expressed	999:1010	arg1	genes					1012:1016	co-expressed genes	999:1016	co-expressed genes with a distinct and opposite correlation between fibre and sugar content	999:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	2	66	theme	carbon	488:493	arg1	sequestration					495:507	carbon sequestration	488:507	carbon sequestration	488:507	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	0	67	from	changes	14:20	arg1	sugarcane					40:48	the developing sugarcane	25:48	the developing sugarcane	25:48	Transcriptome changes in the developing sugarcane culm associated with high yield and early-season high sugar content.
35224663	0	68	theme	high	99:102	arg1	content					110:116	early-season high sugar content	86:116	early-season high sugar content	86:116	Transcriptome changes in the developing sugarcane culm associated with high yield and early-season high sugar content.
35224663	9	69	theme	Unsupervised	1316:1327	arg1	clustering					1342:1351	Unsupervised hierarchical clustering	1316:1351	Unsupervised hierarchical clustering of gene expression	1316:1370	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	3	70	used	utilized	564:571	arg2	KQ228					538:542	KQ228	538:542	KQ228	538:542	Sugarcane varieties such as KQ228 and Q253 are highly utilized in the industry.
35224663	3	70	used	utilized	564:571	arg2	varieties					520:528	Sugarcane varieties	510:528	Sugarcane varieties such as KQ228 and Q253	510:551	Sugarcane varieties such as KQ228 and Q253 are highly utilized in the industry.
35224663	3	70	used	utilized	564:571	arg2	Q253					548:551	Q253	548:551	Q253	548:551	Sugarcane varieties such as KQ228 and Q253 are highly utilized in the industry.
35224663	9	71	theme	development	1461:1471	arg1	stage					1452:1456	the stage	1448:1456	the stage of development	1448:1471	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	10	72	theme	sugar	1695:1699	arg1	species					1701:1707	high sugar species	1690:1707	high sugar species	1690:1707	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	9	73	theme	gene	1356:1359	arg1	expression					1361:1370	gene expression	1356:1370	gene expression	1356:1370	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	2	74	theme	long-term	364:372	arg1	viability					383:391	long-term economic viability	364:391	long-term economic viability	364:391	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	1	75	theme	bio-plastics	260:271	arg1	production					234:243	the production	230:243	the production of bio-energy, bio-plastics and high-value products	230:295	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	10	76	theme	synthetic	1718:1726	arg1	biology					1728:1734	synthetic biology	1718:1734	synthetic biology	1718:1734	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
35224663	1	77	theme	high-value	277:286	arg1	products					288:295	high-value products	277:295	high-value products	277:295	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	6	78	theme	opposite	1038:1045	arg1	correlation					1047:1057	a distinct and opposite correlation	1023:1057	a distinct and opposite correlation between fibre and sugar content	1023:1089	Weighted gene co-expression network analysis (WGCNA) was used and revealed for the first time two sets of co-expressed genes with a distinct and opposite correlation between fibre and sugar content.
35224663	2	79	theme	composition	472:482	arg1	production					450:459	the production	446:459	the production of biomass composition and carbon sequestration	446:507	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	2	80	theme	environmental	397:409	arg1	sustainability					411:424	environmental sustainability	397:424	environmental sustainability	397:424	A crucial challenge for long-term economic viability and environmental sustainability is also to optimize the production of biomass composition and carbon sequestration.
35224663	1	81	contain	has	205:207	arg1	Sugarcane					119:127	Sugarcane	119:127	Sugarcane	119:127	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	1	81	contain	has	205:207	arg2	potential					216:224	a high potential	209:224	a high potential for the production of bio-energy, bio-plastics and high-value products	209:295	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	3	82	theme	Sugarcane	510:518	arg1	Q253					548:551	Q253	548:551	Q253	548:551	Sugarcane varieties such as KQ228 and Q253 are highly utilized in the industry.
35224663	3	82	theme	Sugarcane	510:518	arg1	varieties					520:528	Sugarcane varieties	510:528	Sugarcane varieties such as KQ228 and Q253	510:551	Sugarcane varieties such as KQ228 and Q253 are highly utilized in the industry.
35224663	3	82	theme	Sugarcane	510:518	arg1	KQ228					538:542	KQ228	538:542	KQ228	538:542	Sugarcane varieties such as KQ228 and Q253 are highly utilized in the industry.
35224663	1	83	theme	food	304:307	arg1	industries					328:337	the food and pharmaceutical industries	300:337	the food and pharmaceutical industries	300:337	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	9	84	from	influence	1504:1512	arg1	composition					1525:1535	biomass composition	1517:1535	biomass composition	1517:1535	Unsupervised hierarchical clustering of gene expression revealed a stronger level of segregation associated with the genotypes than the stage of development, suggesting a dominant genetic influence on biomass composition and facilitating breeding selection.
35224663	1	85	theme	high	211:214	arg1	potential					216:224	a high potential	209:224	a high potential for the production of bio-energy, bio-plastics and high-value products	209:295	Sugarcane, with its exceptional carbon dioxide assimilation, biomass and sugar yield, has a high potential for the production of bio-energy, bio-plastics and high-value products in the food and pharmaceutical industries.
35224663	4	86	theme	high	629:632	arg1	content					653:659	a high early-season sugar content	627:659	a high early-season sugar content associated with high yield	627:686	These varieties are characterized by a high early-season sugar content associated with high yield.
35224663	10	87	theme	groups	1604:1609	arg1	Characterization					1574:1589	Characterization	1574:1589	Characterization of these two groups of co-expressed key genes	1574:1635	Characterization of these two groups of co-expressed key genes can help to improve breeding program for high fibre, high sugar species or plant synthetic biology.
36898454	0	0	theme	antibacterial	109:121	arg1	activity					123:130	efficient antibacterial activity	99:130	efficient antibacterial activity for fruit packaging	99:150	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	4	1	theme	porous	683:688	arg1	g-C3N4					690:695	the porous g-C3N4	679:695	the porous g-C3N4	679:695	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	4	1	theme	porous	683:688	arg1	agent					748:752	a photocatalytically-active antibacterial agent	706:752	a photocatalytically-active antibacterial agent	706:752	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	5	2	from	EAB	806:808	arg1	TS					777:778	TS	777:778	TS	777:778	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	5	2	from	EAB	806:808	arg1	elongation					785:794	elongation	785:794	elongation	785:794	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	5	2	from	EAB	806:808	arg1	strength					767:774	The tensile strength	755:774	The tensile strength (TS)	755:779	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	7	3	theme	g-C3N4/CS/PVA	1182:1194	arg1	films					1196:1200	g-C3N4/CS/PVA films	1182:1200	g-C3N4/CS/PVA films at room temperature	1182:1220	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	5	4	theme	tensile	759:765	arg1	TS					777:778	TS	777:778	TS	777:778	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	5	4	theme	tensile	759:765	arg1	strength					767:774	The tensile strength	755:774	The tensile strength (TS)	755:779	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	1	5	theme	good	251:254	arg1	properties					269:278	its good film-forming properties	247:278	its good film-forming properties	247:278	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	0	6	theme	efficient	99:107	arg1	activity					123:130	efficient antibacterial activity	99:130	efficient antibacterial activity for fruit packaging	99:150	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	2	7	contain	have	346:349	arg2	properties					385:394	weak mechanical properties	369:394	weak mechanical properties	369:394	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	2	7	contain	have	346:349	arg2	drawbacks					351:359	drawbacks	351:359	drawbacks such as weak mechanical properties and limited antimicrobial activity	351:429	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	2	7	contain	have	346:349	arg1	films					340:344	pure chitosan films	326:344	pure chitosan films	326:344	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	2	7	contain	have	346:349	arg2	activity					422:429	limited antimicrobial activity	400:429	limited antimicrobial activity	400:429	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	9	8	theme	regenerated	1562:1572	arg1	films					1574:1578	the regenerated films	1558:1578	the regenerated films offering almost identical mechanical properties and activities as the original films	1558:1663	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	1	9	theme	excellent	173:181	arg1	material					187:194	an excellent raw material	170:194	an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability	170:314	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	1	9	theme	excellent	173:181	arg1	Chitosan					153:160	Chitosan	153:160	Chitosan (CS)	153:165	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	1	10	theme	film-forming	256:267	arg1	properties					269:278	its good film-forming properties	247:278	its good film-forming properties	247:278	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	7	11	theme	shelf	1142:1146	arg1	strawberries					1156:1167	The shelf life of strawberries	1138:1167	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature	1138:1220	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	5	12	theme	~10 wt	936:941	arg1	%					942:942	~10 wt%	936:942	~10 wt%	936:942	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	4	13	theme	films	665:669	arg1	properties					632:641	the mechanical properties	617:641	the mechanical properties of the chitosan-based films	617:669	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	8	14	dep	coli	1445:1448	arg1	coli					1454:1457	E. coli	1451:1457	E. coli	1451:1457	The g-C3N4/CS/PVA films offered good antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).
36898454	5	15	theme	optimum	910:916	arg1	loading					925:931	the optimum g-C3N4 loading	906:931	the optimum g-C3N4 loading of ~10 wt%	906:942	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	5	16	theme	%	942:942	arg1	loading					925:931	the optimum g-C3N4 loading	906:931	the optimum g-C3N4 loading of ~10 wt%	906:942	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	3	17	theme	polyvinyl	494:502	arg1	alcohol					504:510	polyvinyl alcohol	494:510	polyvinyl alcohol (PVA)	494:516	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	17	theme	polyvinyl	494:502	arg1	PVA					513:515	PVA	513:515	PVA	513:515	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	5	18	theme	films	832:836	arg1	TS					777:778	TS	777:778	TS	777:778	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	5	18	theme	films	832:836	arg1	elongation					785:794	elongation	785:794	elongation	785:794	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	5	18	theme	films	832:836	arg1	strength					767:774	The tensile strength	755:774	The tensile strength (TS)	755:779	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	6	19	theme	vapor	1065:1069	arg1	WVP					1085:1087	WVP	1085:1087	WVP	1085:1087	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	6	19	theme	vapor	1065:1069	arg1	permeability					1071:1082	the water vapor permeability	1055:1082	the water vapor permeability (WVP)	1055:1088	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	5	20	theme	g-C3N4/CS/PVA	818:830	arg1	films					832:836	the g-C3N4/CS/PVA films	814:836	the g-C3N4/CS/PVA films	814:836	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	0	21	with	films	88:92	arg1	activity					123:130	efficient antibacterial activity	99:130	efficient antibacterial activity for fruit packaging	99:150	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	3	22	theme	graphitic	529:537	arg1	nitride					546:552	porous graphitic carbon nitride	522:552	porous graphitic carbon nitride (g-C3N4)	522:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	22	theme	graphitic	529:537	arg1	g-C3N4					555:560	g-C3N4	555:560	g-C3N4	555:560	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	1	23	theme	raw	183:185	arg1	material					187:194	an excellent raw material	170:194	an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability	170:314	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	1	23	theme	raw	183:185	arg1	Chitosan					153:160	Chitosan	153:160	Chitosan (CS)	153:165	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	0	24	theme	fruit	136:140	arg1	packaging					142:150	fruit packaging	136:150	fruit packaging	136:150	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	9	25	theme	identical	1596:1604	arg1	films					1659:1663	the original films	1646:1663	the original films	1646:1663	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	9	25	theme	identical	1596:1604	arg1	activities					1632:1641	activities	1632:1641	activities	1632:1641	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	9	25	theme	identical	1596:1604	arg1	properties					1617:1626	almost identical mechanical properties	1589:1626	almost identical mechanical properties	1589:1626	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	7	26	from	temperature	1210:1220	arg1	films					1196:1200	g-C3N4/CS/PVA films	1182:1200	g-C3N4/CS/PVA films at room temperature	1182:1220	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	3	27	theme	carbon	539:544	arg1	nitride					546:552	porous graphitic carbon nitride	522:552	porous graphitic carbon nitride (g-C3N4)	522:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	27	theme	carbon	539:544	arg1	g-C3N4					555:560	g-C3N4	555:560	g-C3N4	555:560	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	2	28	theme	limited	400:406	arg1	activity					422:429	limited antimicrobial activity	400:429	limited antimicrobial activity	400:429	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	3	29	theme	porous	522:527	arg1	nitride					546:552	porous graphitic carbon nitride	522:552	porous graphitic carbon nitride (g-C3N4)	522:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	29	theme	porous	522:527	arg1	g-C3N4					555:560	g-C3N4	555:560	g-C3N4	555:560	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	9	30	theme	mechanical	1606:1615	arg1	films					1659:1663	the original films	1646:1663	the original films	1646:1663	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	9	30	theme	mechanical	1606:1615	arg1	activities					1632:1641	activities	1632:1641	activities	1632:1641	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	9	30	theme	mechanical	1606:1615	arg1	properties					1617:1626	almost identical mechanical properties	1589:1626	almost identical mechanical properties	1589:1626	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	4	31	theme	chitosan-based	650:663	arg1	films					665:669	the chitosan-based films	646:669	the chitosan-based films	646:669	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	2	32	theme	pure	326:329	arg1	films					340:344	pure chitosan films	326:344	pure chitosan films	326:344	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	6	33	theme	films	1014:1018	arg1	WCA					1002:1004	WCA	1002:1004	WCA	1002:1004	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	6	33	theme	films	1014:1018	arg1	angle					995:999	the water contact angle	977:999	the water contact angle (WCA) of the films from 38° to 50°	977:1034	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	4	34	theme	photocatalytically-active	708:732	arg1	g-C3N4					690:695	the porous g-C3N4	679:695	the porous g-C3N4	679:695	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	4	34	theme	photocatalytically-active	708:732	arg1	agent					748:752	a photocatalytically-active antibacterial agent	706:752	a photocatalytically-active antibacterial agent	706:752	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	5	35	theme	g-C3N4	918:923	arg1	loading					925:931	the optimum g-C3N4 loading	906:931	the optimum g-C3N4 loading of ~10 wt%	906:942	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	0	36	theme	graphitic	21:29	arg1	alcohol					65:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	7	37	theme	CS/PVA	1335:1340	arg1	films					1342:1346	CS/PVA films	1335:1346	CS/PVA films	1335:1346	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	10	38	theme	antimicrobial	1731:1743	arg1	applications					1755:1766	low-cost antimicrobial packaging applications	1722:1766	low-cost antimicrobial packaging applications	1722:1766	The prepared g-C3N4/CS/PVA films thus offer promise for low-cost antimicrobial packaging applications.
36898454	0	39	theme	recyclable	10:19	arg1	alcohol					65:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	10	40	theme	g-C3N4/CS/PVA	1679:1691	arg1	films					1693:1697	The prepared g-C3N4/CS/PVA films	1666:1697	The prepared g-C3N4/CS/PVA films	1666:1697	The prepared g-C3N4/CS/PVA films thus offer promise for low-cost antimicrobial packaging applications.
36898454	6	41	dep	135 × 10-12 g∙Pa-1 s-1 m-1	1110:1135	arg1	to					1107:1108	to	1107:1108	to	1107:1108	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	5	42	theme	pristine	881:888	arg1	films					897:901	the pristine CS/PVA films	877:901	the pristine CS/PVA films	877:901	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	4	43	theme	mechanical	621:630	arg1	properties					632:641	the mechanical properties	617:641	the mechanical properties of the chitosan-based films	617:669	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	0	44	theme	nitride/chitosan/polyvinyl	38:63	arg1	alcohol					65:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	9	45	theme	composite	1512:1520	arg1	films					1522:1526	the composite films	1508:1526	the composite films	1508:1526	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	3	46	theme	packaging	457:465	arg1	films					467:471	novel food packaging films	446:471	novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4)	446:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	6	47	dep	50°	1032:1034	arg1	to					1029:1030	to	1029:1030	to	1029:1030	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	1	48	theme	food	219:222	arg1	films					234:238	food packaging films	219:238	food packaging films due to its good film-forming properties, non-toxicity, and biodegradability	219:314	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	0	49	theme	carbon	31:36	arg1	alcohol					65:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol	10:71	Green and recyclable graphitic carbon nitride/chitosan/polyvinyl alcohol photocatalytic films with efficient antibacterial activity for fruit packaging.
36898454	6	50	theme	g-C3N4	961:966	arg1	addition					949:956	The addition	945:956	The addition of g-C3N4	945:966	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	7	51	theme	PE	1322:1323	arg1	films					1326:1330	polyethylene (PE) films	1308:1330	polyethylene (PE) films	1308:1330	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	1	52	theme	packaging	224:232	arg1	films					234:238	food packaging films	219:238	food packaging films due to its good film-forming properties, non-toxicity, and biodegradability	219:314	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	9	53	theme	original	1650:1657	arg1	films					1659:1663	the original films	1646:1663	the original films	1646:1663	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	9	53	theme	original	1650:1657	arg1	activities					1632:1641	activities	1632:1641	activities	1632:1641	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	9	53	theme	original	1650:1657	arg1	properties					1617:1626	almost identical mechanical properties	1589:1626	almost identical mechanical properties	1589:1626	Further, the composite films could be easily recycled with the regenerated films offering almost identical mechanical properties and activities as the original films.
36898454	3	54	contain	containing	473:482	arg2	alcohol					504:510	polyvinyl alcohol	494:510	polyvinyl alcohol (PVA)	494:516	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	54	contain	containing	473:482	arg2	chitosan					484:491	chitosan	484:491	chitosan	484:491	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	54	contain	containing	473:482	arg2	PVA					513:515	PVA	513:515	PVA	513:515	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	54	contain	containing	473:482	arg2	nitride					546:552	porous graphitic carbon nitride	522:552	porous graphitic carbon nitride (g-C3N4)	522:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	54	contain	containing	473:482	arg2	g-C3N4					555:560	g-C3N4	555:560	g-C3N4	555:560	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	3	54	contain	containing	473:482	arg1	films					467:471	novel food packaging films	446:471	novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4)	446:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	10	55	theme	packaging	1745:1753	arg1	applications					1755:1766	low-cost antimicrobial packaging applications	1722:1766	low-cost antimicrobial packaging applications	1722:1766	The prepared g-C3N4/CS/PVA films thus offer promise for low-cost antimicrobial packaging applications.
36898454	5	56	theme	CS/PVA	890:895	arg1	films					897:901	the pristine CS/PVA films	877:901	the pristine CS/PVA films	877:901	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	5	57	theme	break	799:803	arg1	EAB					806:808	break (EAB)	799:809	break (EAB)	799:809	The tensile strength (TS) and elongation at break (EAB) of the g-C3N4/CS/PVA films both increased by ~4 times compared to the pristine CS/PVA films at the optimum g-C3N4 loading of ~10 wt%.
36898454	2	58	theme	antimicrobial	408:420	arg1	activity					422:429	limited antimicrobial activity	400:429	limited antimicrobial activity	400:429	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	8	59	theme	antibacterial	1400:1412	arg1	properties					1414:1423	good antibacterial properties	1395:1423	good antibacterial properties	1395:1423	The g-C3N4/CS/PVA films offered good antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).
36898454	7	60	theme	polyethylene	1308:1319	arg1	films					1326:1330	polyethylene (PE) films	1308:1330	polyethylene (PE) films	1308:1330	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	6	61	theme	water	1059:1063	arg1	WVP					1085:1087	WVP	1085:1087	WVP	1085:1087	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	6	61	theme	water	1059:1063	arg1	permeability					1071:1082	the water vapor permeability	1055:1082	the water vapor permeability (WVP)	1055:1088	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	3	62	theme	novel	446:450	arg1	films					467:471	novel food packaging films	446:471	novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4)	446:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	7	63	theme	room	1205:1208	arg1	temperature					1210:1220	room temperature	1205:1220	room temperature	1205:1220	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	8	64	theme	good	1395:1398	arg1	properties					1414:1423	good antibacterial properties	1395:1423	good antibacterial properties	1395:1423	The g-C3N4/CS/PVA films offered good antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).
36898454	6	65	theme	contact	987:993	arg1	WCA					1002:1004	WCA	1002:1004	WCA	1002:1004	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	6	65	theme	contact	987:993	arg1	angle					995:999	the water contact angle	977:999	the water contact angle (WCA) of the films from 38° to 50°	977:1034	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	4	66	theme	antibacterial	734:746	arg1	g-C3N4					690:695	the porous g-C3N4	679:695	the porous g-C3N4	679:695	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	4	66	theme	antibacterial	734:746	arg1	agent					748:752	a photocatalytically-active antibacterial agent	706:752	a photocatalytically-active antibacterial agent	706:752	The PVA served to improve the mechanical properties of the chitosan-based films, whilst the porous g-C3N4 acted as a photocatalytically-active antibacterial agent.
36898454	1	67	theme	films	234:238	arg1	preparation					204:214	the preparation	200:214	the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability	200:314	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	7	68	theme	of	1153:1154	arg1	strawberries					1156:1167	The shelf life of strawberries	1138:1167	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature	1138:1220	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	8	69	theme	g-C3N4/CS/PVA	1367:1379	arg1	films					1381:1385	The g-C3N4/CS/PVA films	1363:1385	The g-C3N4/CS/PVA films	1363:1385	The g-C3N4/CS/PVA films offered good antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).
36898454	6	70	from	50°	1032:1034	arg1	WCA					1002:1004	WCA	1002:1004	WCA	1002:1004	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	6	70	from	50°	1032:1034	arg1	angle					995:999	the water contact angle	977:999	the water contact angle (WCA) of the films from 38° to 50°	977:1034	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	2	71	theme	chitosan	331:338	arg1	films					340:344	pure chitosan films	326:344	pure chitosan films	326:344	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	10	72	theme	low-cost	1722:1729	arg1	applications					1755:1766	low-cost antimicrobial packaging applications	1722:1766	low-cost antimicrobial packaging applications	1722:1766	The prepared g-C3N4/CS/PVA films thus offer promise for low-cost antimicrobial packaging applications.
36898454	1	73	theme	due	240:242	arg1	films					234:238	food packaging films	219:238	food packaging films due to its good film-forming properties, non-toxicity, and biodegradability	219:314	Chitosan (CS) is an excellent raw material for the preparation of food packaging films due to its good film-forming properties, non-toxicity, and biodegradability.
36898454	8	74	dep	aureus	1479:1484	arg1	aureus					1490:1495	S. aureus	1487:1495	S. aureus	1487:1495	The g-C3N4/CS/PVA films offered good antibacterial properties against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus).
36898454	7	75	dep	96 h	1246:1249	arg1	up					1240:1241	up	1240:1241	up	1240:1241	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
36898454	2	76	theme	mechanical	374:383	arg1	properties					385:394	weak mechanical properties	369:394	weak mechanical properties	369:394	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	3	77	theme	food	452:455	arg1	films					467:471	novel food packaging films	446:471	novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4)	446:561	In this work, novel food packaging films containing chitosan, polyvinyl alcohol (PVA) and porous graphitic carbon nitride (g-C3N4) were successfully prepared.
36898454	6	78	theme	water	981:985	arg1	WCA					1002:1004	WCA	1002:1004	WCA	1002:1004	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	6	78	theme	water	981:985	arg1	angle					995:999	the water contact angle	977:999	the water contact angle (WCA) of the films from 38° to 50°	977:1034	The addition of g-C3N4 increase the water contact angle (WCA) of the films from 38° to 50°, whilst decreasing the water vapor permeability (WVP) from 160 × 10-12 to 135 × 10-12 g∙Pa-1 s-1 m-1.
36898454	10	79	theme	prepared	1670:1677	arg1	films					1693:1697	The prepared g-C3N4/CS/PVA films	1666:1697	The prepared g-C3N4/CS/PVA films	1666:1697	The prepared g-C3N4/CS/PVA films thus offer promise for low-cost antimicrobial packaging applications.
36898454	2	80	theme	weak	369:372	arg1	properties					385:394	weak mechanical properties	369:394	weak mechanical properties	369:394	However, pure chitosan films have drawbacks such as weak mechanical properties and limited antimicrobial activity.
36898454	7	81	theme	life	1148:1151	arg1	strawberries					1156:1167	The shelf life of strawberries	1138:1167	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature	1138:1220	The shelf life of strawberries covered with g-C3N4/CS/PVA films at room temperature could be extended up to 96 h, compared to 48 h and 72 h for strawberries covered with polyethylene (PE) films or CS/PVA films, respectively.
35327264	1	0	theme	wall	314:317	arg1	activities					353:362	activities	353:362	activities	353:362	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	0	theme	wall	314:317	arg1	compositions					289:300	fruit, the ultrastructure and polysaccharides compositions	243:300	fruit, the ultrastructure and polysaccharides compositions	243:300	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	0	theme	wall	314:317	arg1	quality					341:347	the fruit quality	331:347	the fruit quality	331:347	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	4	1	theme	polymers	1035:1042	arg1	production					1002:1011	the production	998:1011	the production of new macromolecular polymers	998:1042	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	5	2	from	stages	1369:1374	arg1	softening					1346:1354	fruit softening	1340:1354	fruit softening at different stages of storage	1340:1385	Polygalacturonase, pectin methylesterase, xyloglucan endo-transglycosylase/hydrolase, α-arabinofuranosidase, β-galactosidase, and cellulase were associated with fruit softening at different stages of storage.
35327264	6	3	theme	similar	1399:1405	arg1	mechanisms					1417:1426	similar softening mechanisms	1399:1426	similar softening mechanisms	1399:1426	There were similar softening mechanisms in the three Hami melons.
35327264	1	4	theme	carbohydrate	216:227	arg1	metabolism					229:238	cell wall carbohydrate metabolism	206:238	cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels	206:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	4	5	dep	weight	743:748	arg1	The					729:731	The	729:731	The	729:731	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	6	theme	macromolecular	1020:1033	arg1	polymers					1035:1042	new macromolecular polymers	1016:1042	new macromolecular polymers	1016:1042	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	0	7	theme	Softening	85:93	arg1	Process					95:101	the Softening Process	81:101	the Softening Process of Hami Melon	81:115	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	4	8	theme	molecular	733:741	arg1	weight					743:748	molecular weight	733:748	molecular weight	733:748	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	5	9	theme	storage	1379:1385	arg1	stages					1369:1374	different stages	1359:1374	different stages of storage	1359:1385	Polygalacturonase, pectin methylesterase, xyloglucan endo-transglycosylase/hydrolase, α-arabinofuranosidase, β-galactosidase, and cellulase were associated with fruit softening at different stages of storage.
35327264	4	10	theme	new	1016:1018	arg1	polymers					1035:1042	new macromolecular polymers	1016:1042	new macromolecular polymers	1016:1042	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	1	11	theme	fruit	243:247	arg1	compositions					289:300	fruit, the ultrastructure and polysaccharides compositions	243:300	fruit, the ultrastructure and polysaccharides compositions	243:300	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	5	12	theme	xyloglucan	1221:1230	arg1	endo-transglycosylase/hydrolase					1232:1262	xyloglucan endo-transglycosylase/hydrolase	1221:1262	xyloglucan endo-transglycosylase/hydrolase	1221:1262	Polygalacturonase, pectin methylesterase, xyloglucan endo-transglycosylase/hydrolase, α-arabinofuranosidase, β-galactosidase, and cellulase were associated with fruit softening at different stages of storage.
35327264	7	13	theme	further	1492:1498	arg1	study					1500:1504	further study	1492:1504	further study on the fruit softening mechanisms of Hami melons	1492:1553	This study will provide reference for further study on the fruit softening mechanisms of Hami melons.
35327264	3	14	theme	better	674:679	arg1	quality					689:695	a better storage quality	672:695	a better storage quality	672:695	'Chougua' showed a better storage quality and intact cell wall structure.
35327264	3	15	theme	storage	681:687	arg1	quality					689:695	a better storage quality	672:695	a better storage quality	672:695	'Chougua' showed a better storage quality and intact cell wall structure.
35327264	5	16	theme	fruit	1340:1344	arg1	softening					1346:1354	fruit softening	1340:1354	fruit softening at different stages of storage	1340:1385	Polygalacturonase, pectin methylesterase, xyloglucan endo-transglycosylase/hydrolase, α-arabinofuranosidase, β-galactosidase, and cellulase were associated with fruit softening at different stages of storage.
35327264	1	17	dep	varieties	438:446	arg1	Jinhuami					465:472	Jinhuami	465:472	Jinhuami	465:472	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	17	dep	varieties	438:446	arg1	Xizhoumi					450:457	Xizhoumi	450:457	Xizhoumi	450:457	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	17	dep	varieties	438:446	arg1	Chougua					484:490	'Chougua'	483:491	'Chougua'	483:491	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	17	dep	varieties	438:446	arg1	varieties					438:446	three Hami melon varieties	421:446	three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels	421:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	4	18	from	depolymerization	939:954	arg1	monosaccharides					1078:1092	pectin monosaccharides	1071:1092	pectin monosaccharides (glucose, galactose, and arabinose)	1071:1128	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	18	from	depolymerization	939:954	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	18	from	depolymerization	939:954	arg1	arabinose					1119:1127	arabinose	1119:1127	arabinose	1119:1127	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	18	from	depolymerization	939:954	arg1	glucose					1095:1101	glucose	1095:1101	glucose	1095:1101	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	19	theme	pectin	889:894	arg1	polysaccharides					896:910	pectin polysaccharides	889:910	pectin polysaccharides	889:910	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	1	20	theme	fruit	335:339	arg1	quality					341:347	the fruit quality	331:347	the fruit quality	331:347	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	2	21	theme	lowest	626:631	arg1	incidence					639:647	the lowest decay incidence	622:647	the lowest decay incidence (0%)	622:652	The results showed that 'Chougua' maintained a higher firmness on day 18, with the lowest decay incidence (0%).
35327264	2	21	theme	lowest	626:631	arg1	%					651:651	0%	650:651	0%	650:651	The results showed that 'Chougua' maintained a higher firmness on day 18, with the lowest decay incidence (0%).
35327264	7	22	from	study	1500:1504	arg1	mechanisms					1529:1538	the fruit softening mechanisms	1509:1538	the fruit softening mechanisms of Hami melons	1509:1553	This study will provide reference for further study on the fruit softening mechanisms of Hami melons.
35327264	1	23	theme	Hami	427:430	arg1	varieties					438:446	three Hami melon varieties	421:446	three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels	421:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	23	theme	Hami	427:430	arg1	Xizhoumi					450:457	Xizhoumi	450:457	Xizhoumi	450:457	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	23	theme	Hami	427:430	arg1	Chougua					484:490	'Chougua'	483:491	'Chougua'	483:491	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	23	theme	Hami	427:430	arg1	Jinhuami					465:472	Jinhuami	465:472	Jinhuami	465:472	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	0	24	dep	Changes	70:76	arg1	melo					126:129	melo	126:129	melo	126:129	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	4	25	theme	macromolecular	959:972	arg1	polymers					974:981	macromolecular polymers	959:981	macromolecular polymers accompanied by the production of new macromolecular polymers	959:1042	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	0	26	theme	Cell	12:15	arg1	Polysaccharides					22:36	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	26	theme	Cell	12:15	arg1	Disassembly					38:48	Disassembly	38:48	Disassembly	38:48	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	26	theme	Cell	12:15	arg1	Activity					61:68	Enzyme Activity	54:68	Enzyme Activity	54:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	27	theme	Melon	111:115	arg1	Process					95:101	the Softening Process	81:101	the Softening Process of Hami Melon	81:115	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	1	28	theme	melon	432:436	arg1	varieties					438:446	three Hami melon varieties	421:446	three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels	421:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	28	theme	melon	432:436	arg1	Xizhoumi					450:457	Xizhoumi	450:457	Xizhoumi	450:457	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	28	theme	melon	432:436	arg1	Chougua					484:490	'Chougua'	483:491	'Chougua'	483:491	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	28	theme	melon	432:436	arg1	Jinhuami					465:472	Jinhuami	465:472	Jinhuami	465:472	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	5	29	theme	pectin	1198:1203	arg1	methylesterase					1205:1218	pectin methylesterase	1198:1218	pectin methylesterase	1198:1218	Polygalacturonase, pectin methylesterase, xyloglucan endo-transglycosylase/hydrolase, α-arabinofuranosidase, β-galactosidase, and cellulase were associated with fruit softening at different stages of storage.
35327264	4	30	theme	polysaccharides	896:910	arg1	obvious					916:922	obvious	916:922	obvious	916:922	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	30	theme	polysaccharides	896:910	arg1	degradation					874:884	the degradation	870:884	the degradation of pectin polysaccharides	870:910	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	1	31	theme	ultrastructure	254:267	arg1	compositions					289:300	fruit, the ultrastructure and polysaccharides compositions	243:300	fruit, the ultrastructure and polysaccharides compositions	243:300	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	0	32	theme	Hami	106:109	arg1	Melon					111:115	Hami Melon	106:115	Hami Melon	106:115	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	4	33	theme	melons	1171:1176	arg1	process					1151:1157	the softening process	1137:1157	the softening process of the Hami melons	1137:1176	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	6	34	theme	Hami	1441:1444	arg1	melons					1446:1451	the three Hami melons	1431:1451	the three Hami melons	1431:1451	There were similar softening mechanisms in the three Hami melons.
35327264	4	35	dep	monosaccharides	1078:1092	arg1	monosaccharides					1078:1092	pectin monosaccharides	1071:1092	pectin monosaccharides (glucose, galactose, and arabinose)	1071:1128	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	35	dep	monosaccharides	1078:1092	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	35	dep	monosaccharides	1078:1092	arg1	arabinose					1119:1127	arabinose	1119:1127	arabinose	1119:1127	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	35	dep	monosaccharides	1078:1092	arg1	glucose					1095:1101	glucose	1095:1101	glucose	1095:1101	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	0	36	theme	Polysaccharides	22:36	arg1	Role					4:7	The Role	0:7	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity	0:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	4	37	theme	polysaccharides	794:808	arg1	weight					743:748	molecular weight	733:748	molecular weight	733:748	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	37	theme	polysaccharides	794:808	arg1	composition					769:779	monosaccharide composition	754:779	monosaccharide composition	754:779	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	38	theme	Hami	1166:1169	arg1	melons					1171:1176	the Hami melons	1162:1176	the Hami melons	1162:1176	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	3	39	theme	intact	701:706	arg1	structure					718:726	intact cell wall structure	701:726	intact cell wall structure	701:726	'Chougua' showed a better storage quality and intact cell wall structure.
35327264	1	40	theme	molecular	173:181	arg1	properties					183:192	molecular properties	173:192	molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels	173:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	0	41	theme	Wall	17:20	arg1	Polysaccharides					22:36	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	41	theme	Wall	17:20	arg1	Disassembly					38:48	Disassembly	38:48	Disassembly	38:48	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	41	theme	Wall	17:20	arg1	Activity					61:68	Enzyme Activity	54:68	Enzyme Activity	54:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	1	42	theme	polysaccharides	273:287	arg1	compositions					289:300	fruit, the ultrastructure and polysaccharides compositions	243:300	fruit, the ultrastructure and polysaccharides compositions	243:300	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	43	from	metabolism	229:238	arg1	compositions					289:300	fruit, the ultrastructure and polysaccharides compositions	243:300	fruit, the ultrastructure and polysaccharides compositions	243:300	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	43	from	metabolism	229:238	arg1	quality					341:347	the fruit quality	331:347	the fruit quality	331:347	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	7	44	theme	Hami	1543:1546	arg1	melons					1548:1553	Hami melons	1543:1553	Hami melons	1543:1553	This study will provide reference for further study on the fruit softening mechanisms of Hami melons.
35327264	7	45	theme	melons	1548:1553	arg1	mechanisms					1529:1538	the fruit softening mechanisms	1509:1538	the fruit softening mechanisms of Hami melons	1509:1553	This study will provide reference for further study on the fruit softening mechanisms of Hami melons.
35327264	4	46	theme	Hami	814:817	arg1	melons					819:824	Hami melons	814:824	Hami melons	814:824	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	47	theme	great	836:840	arg1	changes					842:848	great changes	836:848	great changes	836:848	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	0	48	dep	Polysaccharides	22:36	arg1	Polysaccharides					22:36	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	48	dep	Polysaccharides	22:36	arg1	Disassembly					38:48	Disassembly	38:48	Disassembly	38:48	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	48	dep	Polysaccharides	22:36	arg1	Activity					61:68	Enzyme Activity	54:68	Enzyme Activity	54:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	4	49	theme	pectin	1071:1076	arg1	monosaccharides					1078:1092	pectin monosaccharides	1071:1092	pectin monosaccharides (glucose, galactose, and arabinose)	1071:1128	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	49	theme	pectin	1071:1076	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	49	theme	pectin	1071:1076	arg1	arabinose					1119:1127	arabinose	1119:1127	arabinose	1119:1127	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	49	theme	pectin	1071:1076	arg1	glucose					1095:1101	glucose	1095:1101	glucose	1095:1101	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	50	theme	wall	789:792	arg1	polysaccharides					794:808	cell wall polysaccharides	784:808	cell wall polysaccharides	784:808	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	51	theme	cell	784:787	arg1	polysaccharides					794:808	cell wall polysaccharides	784:808	cell wall polysaccharides	784:808	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	1	52	theme	enzymes	367:373	arg1	activities					353:362	activities	353:362	activities	353:362	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	52	theme	enzymes	367:373	arg1	compositions					289:300	fruit, the ultrastructure and polysaccharides compositions	243:300	fruit, the ultrastructure and polysaccharides compositions	243:300	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	52	theme	enzymes	367:373	arg1	quality					341:347	the fruit quality	331:347	the fruit quality	331:347	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	3	53	theme	cell	708:711	arg1	structure					718:726	intact cell wall structure	701:726	intact cell wall structure	701:726	'Chougua' showed a better storage quality and intact cell wall structure.
35327264	5	54	theme	different	1359:1367	arg1	stages					1369:1374	different stages	1359:1374	different stages of storage	1359:1385	Polygalacturonase, pectin methylesterase, xyloglucan endo-transglycosylase/hydrolase, α-arabinofuranosidase, β-galactosidase, and cellulase were associated with fruit softening at different stages of storage.
35327264	4	55	theme	softening	1141:1149	arg1	process					1151:1157	the softening process	1137:1157	the softening process of the Hami melons	1137:1176	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	2	56	theme	higher	590:595	arg1	firmness					597:604	a higher firmness	588:604	a higher firmness	588:604	The results showed that 'Chougua' maintained a higher firmness on day 18, with the lowest decay incidence (0%).
35327264	3	57	theme	wall	713:716	arg1	structure					718:726	intact cell wall structure	701:726	intact cell wall structure	701:726	'Chougua' showed a better storage quality and intact cell wall structure.
35327264	7	58	theme	softening	1519:1527	arg1	mechanisms					1529:1538	the fruit softening mechanisms	1509:1538	the fruit softening mechanisms of Hami melons	1509:1553	This study will provide reference for further study on the fruit softening mechanisms of Hami melons.
35327264	4	59	theme	monosaccharide	754:767	arg1	composition					769:779	monosaccharide composition	754:779	monosaccharide composition	754:779	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	60	from	changes	1060:1066	arg1	monosaccharides					1078:1092	pectin monosaccharides	1071:1092	pectin monosaccharides (glucose, galactose, and arabinose)	1071:1128	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	60	from	changes	1060:1066	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	60	from	changes	1060:1066	arg1	arabinose					1119:1127	arabinose	1119:1127	arabinose	1119:1127	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	60	from	changes	1060:1066	arg1	glucose					1095:1101	glucose	1095:1101	glucose	1095:1101	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	61	theme	composition	1048:1058	arg1	changes					1060:1066	composition changes	1048:1066	composition changes in pectin monosaccharides (glucose, galactose, and arabinose)	1048:1128	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	0	62	dep	melo	126:129	arg1	L					131:131	L	131:131	Cucumis melo L.	118:132	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	4	63	theme	polymers	974:981	arg1	changes					1060:1066	composition changes	1048:1066	composition changes in pectin monosaccharides (glucose, galactose, and arabinose)	1048:1128	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	4	63	theme	polymers	974:981	arg1	depolymerization					939:954	the depolymerization	935:954	the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers	935:1042	The molecular weight and monosaccharide composition of cell wall polysaccharides for Hami melons underwent great changes during storage, and the degradation of pectin polysaccharides was obvious, involving the depolymerization of macromolecular polymers accompanied by the production of new macromolecular polymers and composition changes in pectin monosaccharides (glucose, galactose, and arabinose) during the softening process of the Hami melons.
35327264	2	64	theme	decay	633:637	arg1	incidence					639:647	the lowest decay incidence	622:647	the lowest decay incidence (0%)	622:652	The results showed that 'Chougua' maintained a higher firmness on day 18, with the lowest decay incidence (0%).
35327264	2	64	theme	decay	633:637	arg1	%					651:651	0%	650:651	0%	650:651	The results showed that 'Chougua' maintained a higher firmness on day 18, with the lowest decay incidence (0%).
35327264	1	65	theme	fruit	387:391	arg1	softening					393:401	fruit softening	387:401	fruit softening	387:401	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	66	dep	physiological	155:167	arg1	the					151:153	the	151:153	the	151:153	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	6	67	theme	softening	1407:1415	arg1	mechanisms					1417:1426	similar softening mechanisms	1399:1426	similar softening mechanisms	1399:1426	There were similar softening mechanisms in the three Hami melons.
35327264	1	68	theme	different	513:521	arg1	levels					535:540	three different storability levels	507:540	three different storability levels	507:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	69	theme	cell	206:209	arg1	metabolism					229:238	cell wall carbohydrate metabolism	206:238	cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels	206:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	0	70	theme	Enzyme	54:59	arg1	Polysaccharides					22:36	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	Cell Wall Polysaccharides Disassembly and Enzyme Activity	12:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	0	70	theme	Enzyme	54:59	arg1	Activity					61:68	Enzyme Activity	54:68	Enzyme Activity	54:68	The Role of Cell Wall Polysaccharides Disassembly and Enzyme Activity Changes in the Softening Process of Hami Melon (Cucumis melo L.).
35327264	1	71	theme	cell	309:312	arg1	wall					314:317	the cell wall	305:317	the cell wall	305:317	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	7	72	theme	fruit	1513:1517	arg1	mechanisms					1529:1538	the fruit softening mechanisms	1509:1538	the fruit softening mechanisms of Hami melons	1509:1553	This study will provide reference for further study on the fruit softening mechanisms of Hami melons.
35327264	1	73	theme	storability	523:533	arg1	levels					535:540	three different storability levels	507:540	three different storability levels	507:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
35327264	1	74	theme	wall	211:214	arg1	metabolism					229:238	cell wall carbohydrate metabolism	206:238	cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels	206:540	To investigate the physiological and molecular properties relating to cell wall carbohydrate metabolism in fruit, the ultrastructure and polysaccharides compositions of the cell wall, as well as the fruit quality and activities of enzymes relating to fruit softening, were studied for three Hami melon varieties ('Xizhoumi 17', 'Jinhuami 25', and 'Chougua') representing three different storability levels.
36794816	6	0	from	infantis	715:722	arg1	infants					748:754	Kenyan infants	741:754	Kenyan infants (80%)	741:760	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	6	0	from	infantis	715:722	arg1	%					759:759	80%	757:759	80%	757:759	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	0	1	with	association	72:82	arg1	oligosaccharides					100:115	human milk oligosaccharides	89:115	human milk oligosaccharides	89:115	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	11	2	theme	HMO-gut	1696:1702	arg1	association					1715:1725	a specific HMO-gut microbiome association	1685:1725	a specific HMO-gut microbiome association	1685:1725	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	12	3	theme	modern	1834:1839	arg1	factors					1861:1867	modern microbiome-altering factors	1834:1867	modern microbiome-altering factors	1834:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	11	4	theme	gut	1457:1459	arg1	microbiome					1461:1470	the gut microbiome	1453:1470	the gut microbiome of partially breastfed Kenyan infants over the age of six months	1453:1535	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	10	5	theme	polymorphisms	1269:1281	arg1	basis					1241:1245	the basis	1237:1245	the basis of secretor and Lewis polymorphisms	1237:1281	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	11	6	theme	specific	1687:1694	arg1	association					1715:1725	a specific HMO-gut microbiome association	1685:1725	a specific HMO-gut microbiome association	1685:1725	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	2	7	from	differences	325:335	arg1	microbiome					348:357	the gut microbiome	340:357	the gut microbiome	340:357	Lifestyle and rurality have been shown to contribute to differences in the gut microbiome, including Bifidobacterium levels, between infants.
36794816	10	8	theme	HM	1315:1316	arg1	group					1318:1322	HM group III	1315:1326	HM group III (Se+, Le-) (22%)	1315:1343	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	10	8	theme	HM	1315:1316	arg1	Se+					1329:1331	Se+	1329:1331	Se+	1329:1331	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	10	8	theme	HM	1315:1316	arg1	%					1342:1342	22%	1340:1342	22%	1340:1342	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	11	9	theme	high	1633:1636	arg1	prevalence					1638:1647	the high prevalence	1629:1647	the high prevalence of a specific HM group	1629:1670	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	6	10	theme	Kenyan	741:746	arg1	infants					748:754	Kenyan infants	741:754	Kenyan infants (80%)	741:760	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	6	10	theme	Kenyan	741:746	arg1	%					759:759	80%	757:759	80%	757:759	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	9	11	theme	genes	1084:1088	arg1	pH					1056:1057	a lower pH	1048:1057	a lower pH	1048:1057	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	9	11	theme	genes	1084:1088	arg1	abundance					1071:1079	a lower abundance	1063:1079	a lower abundance of genes encoding pathogenic features	1063:1117	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	11	12	theme	specific	1654:1661	arg1	group					1666:1670	a specific HM group	1652:1670	a specific HM group	1652:1670	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	5	13	theme	B.	698:699	arg1	subsp					708:712	B. longum subsp	698:712	B. longum subsp	698:712	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	12	14	theme	understudied	1785:1796	arg1	population					1798:1807	an understudied population	1782:1807	an understudied population with limited exposure to modern microbiome-altering factors	1782:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	6	15	from	co-existence	776:787	arg1	infants					748:754	Kenyan infants	741:754	Kenyan infants (80%)	741:760	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	6	15	from	co-existence	776:787	arg1	%					759:759	80%	757:759	80%	757:759	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	10	16	theme	oligosaccharides	1146:1161	arg1	analysis					1123:1130	An analysis	1120:1130	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms	1120:1281	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	10	17	theme	human	1135:1139	arg1	oligosaccharides					1146:1161	human milk oligosaccharides	1135:1161	human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms	1135:1281	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	10	17	theme	human	1135:1139	arg1	HMOs					1164:1167	HMOs	1164:1167	HMOs	1164:1167	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	11	18	theme	Bifidobacterium	1570:1584	arg1	community					1586:1594	the Bifidobacterium community	1566:1594	the Bifidobacterium community	1566:1594	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	0	19	theme	rural	56:60	arg1	Kenya					62:66	rural Kenya	56:66	rural Kenya	56:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	20	from	function	15:22	arg1	infants					43:49	infants	43:49	infants from rural Kenya	43:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	20	from	function	15:22	arg1	Kenya					62:66	rural Kenya	56:66	rural Kenya	56:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	9	21	theme	pathogenic	1099:1108	arg1	features					1110:1117	pathogenic features	1099:1117	pathogenic features	1099:1117	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	5	22	theme	subsp	708:712	arg1	prevalence					684:693	a high prevalence	677:693	a high prevalence of B. longum subsp	677:712	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	9	23	contain	had	1044:1046	arg2	abundance					1071:1079	a lower abundance	1063:1079	a lower abundance of genes encoding pathogenic features	1063:1117	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	9	23	contain	had	1044:1046	arg1	types					967:971	GMC types	963:971	GMC types with a higher prevalence of B. infantis and abundance of B. breve	963:1037	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	9	23	contain	had	1044:1046	arg2	pH					1056:1057	a lower pH	1048:1057	a lower pH	1048:1057	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	12	24	theme	gut	1754:1756	arg1	variation					1769:1777	gut microbiome variation	1754:1777	gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors	1754:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	0	25	theme	milk	95:98	arg1	oligosaccharides					100:115	human milk oligosaccharides	89:115	human milk oligosaccharides	89:115	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	26	from	composition	28:38	arg1	infants					43:49	infants	43:49	infants from rural Kenya	43:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	26	from	composition	28:38	arg1	Kenya					62:66	rural Kenya	56:66	rural Kenya	56:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	10	27	theme	human	1185:1189	arg1	samples					1201:1207	the human milk (HM) samples	1181:1207	the human milk (HM) samples	1181:1207	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	2	28	theme	Bifidobacterium	370:384	arg1	levels					386:391	Bifidobacterium levels	370:391	Bifidobacterium levels	370:391	Lifestyle and rurality have been shown to contribute to differences in the gut microbiome, including Bifidobacterium levels, between infants.
36794816	9	29	theme	breve	1033:1037	arg1	abundance					1017:1025	abundance	1017:1025	abundance of B. breve	1017:1037	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	9	29	theme	breve	1033:1037	arg1	prevalence					987:996	a higher prevalence	978:996	a higher prevalence of B. infantis	978:1011	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	6	30	theme	B.	813:814	arg1	subsp					823:827	B. longum subsp	813:827	B. longum subsp	813:827	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	4	31	theme	Shotgun	535:541	arg1	metagenomics					543:554	Shotgun metagenomics	535:554	Shotgun metagenomics	535:554	Shotgun metagenomics showed Bifidobacterium longum to be the dominant species.
36794816	6	32	theme	subspecies	797:806	arg1	co-existence					776:787	possible co-existence	767:787	possible co-existence of this subspecies with B. longum subsp	767:827	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	6	32	theme	subspecies	797:806	arg1	infantis					715:722	infantis	715:722	infantis (B. infantis) in Kenyan infants (80%)	715:760	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	8	33	theme	microbiome	864:873	arg1	Stratification					838:851	Stratification	838:851	Stratification of the gut microbiome into community (GMC) types	838:900	Stratification of the gut microbiome into community (GMC) types revealed differences in composition and functional features.
36794816	10	34	theme	studied	1369:1375	arg1	populations					1377:1387	most previously studied populations	1353:1387	most previously studied populations	1353:1387	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	11	35	theme	months	1530:1535	arg1	age					1519:1521	the age	1515:1521	the age of six months	1515:1535	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	0	36	with	function	15:22	arg1	oligosaccharides					100:115	human milk oligosaccharides	89:115	human milk oligosaccharides	89:115	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	3	37	theme	microbiomes	475:485	arg1	composition					426:436	composition	426:436	composition	426:436	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	3	37	theme	microbiomes	475:485	arg1	variability					452:462	variability	452:462	variability	452:462	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	3	37	theme	microbiomes	475:485	arg1	function					439:446	function	439:446	function	439:446	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	11	38	theme	group	1666:1670	arg1	prevalence					1638:1647	the high prevalence	1629:1647	the high prevalence of a specific HM group	1629:1670	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	6	39	theme	possible	767:774	arg1	co-existence					776:787	possible co-existence	767:787	possible co-existence of this subspecies with B. longum subsp	767:827	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	6	40	dep	infantis	715:722	arg1	infantis					728:735	B. infantis	725:735	B. infantis	725:735	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	10	41	theme	secretor	1250:1257	arg1	polymorphisms					1269:1281	secretor and Lewis polymorphisms	1250:1281	polymorphisms	1269:1281	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	11	42	theme	microbiome	1704:1713	arg1	association					1715:1725	a specific HMO-gut microbiome association	1685:1725	a specific HMO-gut microbiome association	1685:1725	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	4	43	theme	dominant	596:603	arg1	species					605:611	the dominant species	592:611	the dominant species	592:611	Shotgun metagenomics showed Bifidobacterium longum to be the dominant species.
36794816	11	44	theme	infants	1502:1508	arg1	microbiome					1461:1470	the gut microbiome	1453:1470	the gut microbiome of partially breastfed Kenyan infants over the age of six months	1453:1535	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	0	45	with	composition	28:38	arg1	oligosaccharides					100:115	human milk oligosaccharides	89:115	human milk oligosaccharides	89:115	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	2	46	theme	gut	344:346	arg1	microbiome					348:357	the gut microbiome	340:357	the gut microbiome	340:357	Lifestyle and rurality have been shown to contribute to differences in the gut microbiome, including Bifidobacterium levels, between infants.
36794816	1	47	theme	short-	240:245	arg1	health					261:266	short- and long-term health	240:266	short- and long-term health	240:266	The gut microbiota evolves rapidly after birth, responding dynamically to environmental factors and playing a key role in short- and long-term health.
36794816	11	48	theme	breastfed	1485:1493	arg1	infants					1502:1508	partially breastfed Kenyan infants	1475:1508	partially breastfed Kenyan infants	1475:1508	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	5	49	theme	longum	642:647	arg1	analysis					627:634	A pangenomic analysis	614:634	A pangenomic analysis of B. longum in gut metagenomes	614:666	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	1	50	theme	long-term	251:259	arg1	health					261:266	short- and long-term health	240:266	short- and long-term health	240:266	The gut microbiota evolves rapidly after birth, responding dynamically to environmental factors and playing a key role in short- and long-term health.
36794816	9	51	theme	lower	1050:1054	arg1	pH					1056:1057	a lower pH	1048:1057	a lower pH	1048:1057	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	10	52	theme	Lewis	1263:1267	arg1	polymorphisms					1269:1281	secretor and Lewis polymorphisms	1250:1281	polymorphisms	1269:1281	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	3	53	theme	infants	516:522	arg1	microbiomes					475:485	the gut microbiomes	467:485	the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105)	467:532	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	12	54	theme	microbiome-altering	1841:1859	arg1	factors					1861:1867	modern microbiome-altering factors	1834:1867	modern microbiome-altering factors	1834:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	8	55	theme	functional	942:951	arg1	features					953:960	functional features	942:960	functional features	942:960	Stratification of the gut microbiome into community (GMC) types revealed differences in composition and functional features.
36794816	5	56	theme	pangenomic	616:625	arg1	analysis					627:634	A pangenomic analysis	614:634	A pangenomic analysis of B. longum in gut metagenomes	614:666	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	5	57	theme	high	679:682	arg1	prevalence					684:693	a high prevalence	677:693	a high prevalence of B. longum subsp	677:712	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	10	58	theme	higher	1294:1299	arg1	prevalence					1301:1310	a higher prevalence	1292:1310	a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations	1292:1387	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	1	59	theme	environmental	192:204	arg1	factors					206:212	environmental factors	192:212	environmental factors	192:212	The gut microbiota evolves rapidly after birth, responding dynamically to environmental factors and playing a key role in short- and long-term health.
36794816	12	60	theme	limited	1814:1820	arg1	exposure					1822:1829	limited exposure	1814:1829	limited exposure to modern microbiome-altering factors	1814:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	0	61	from	association	72:82	arg1	infants					43:49	infants	43:49	infants from rural Kenya	43:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	61	from	association	72:82	arg1	Kenya					62:66	rural Kenya	56:66	rural Kenya	56:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	62	from	Kenya	62:66	arg1	function					15:22	function	15:22	function	15:22	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	62	from	Kenya	62:66	arg1	association					72:82	association	72:82	association with human milk oligosaccharides	72:115	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	62	from	Kenya	62:66	arg1	infants					43:49	infants	43:49	infants from rural Kenya	43:66	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	0	62	from	Kenya	62:66	arg1	composition					28:38	composition	28:38	composition	28:38	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	11	63	theme	HM	1663:1664	arg1	group					1666:1670	a specific HM group	1652:1670	a specific HM group	1652:1670	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	5	64	theme	gut	652:654	arg1	metagenomes					656:666	gut metagenomes	652:666	gut metagenomes	652:666	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	11	65	from	community	1586:1594	arg1	bacteria					1552:1559	bacteria	1552:1559	bacteria from the Bifidobacterium community	1552:1594	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	9	66	theme	GMC	963:965	arg1	types					967:971	GMC types	963:971	GMC types with a higher prevalence of B. infantis and abundance of B. breve	963:1037	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	1	67	theme	gut	122:124	arg1	microbiota					126:135	The gut microbiota	118:135	The gut microbiota	118:135	The gut microbiota evolves rapidly after birth, responding dynamically to environmental factors and playing a key role in short- and long-term health.
36794816	10	68	theme	milk	1141:1144	arg1	oligosaccharides					1146:1161	human milk oligosaccharides	1135:1161	human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms	1135:1281	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	10	68	theme	milk	1141:1144	arg1	HMOs					1164:1167	HMOs	1164:1167	HMOs	1164:1167	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	5	69	dep	B.	698:699	arg1	longum					701:706	longum	701:706	longum	701:706	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	9	70	theme	higher	980:985	arg1	prevalence					987:996	a higher prevalence	978:996	a higher prevalence of B. infantis	978:1011	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	0	71	theme	human	89:93	arg1	oligosaccharides					100:115	human milk oligosaccharides	89:115	human milk oligosaccharides	89:115	Gut microbiome function and composition in infants from rural Kenya and association with human milk oligosaccharides.
36794816	12	72	with	population	1798:1807	arg1	exposure					1822:1829	limited exposure	1814:1829	limited exposure to modern microbiome-altering factors	1814:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	12	73	theme	microbiome	1758:1767	arg1	variation					1769:1777	gut microbiome variation	1754:1777	gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors	1754:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	10	74	theme	group	1318:1322	arg1	prevalence					1301:1310	a higher prevalence	1292:1310	a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations	1292:1387	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	9	75	theme	infantis	1004:1011	arg1	abundance					1017:1025	abundance	1017:1025	abundance of B. breve	1017:1037	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	9	75	theme	infantis	1004:1011	arg1	prevalence					987:996	a higher prevalence	978:996	a higher prevalence of B. infantis	978:1011	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	10	76	theme	HM	1197:1198	arg1	samples					1201:1207	the human milk (HM) samples	1181:1207	the human milk (HM) samples	1181:1207	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	6	77	with	subspecies	797:806	arg1	subsp					823:827	B. longum subsp	813:827	B. longum subsp	813:827	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	10	78	theme	milk	1191:1194	arg1	samples					1201:1207	the human milk (HM) samples	1181:1207	the human milk (HM) samples	1181:1207	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	9	79	with	types	967:971	arg1	abundance					1017:1025	abundance	1017:1025	abundance of B. breve	1017:1037	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	9	79	with	types	967:971	arg1	prevalence					987:996	a higher prevalence	978:996	a higher prevalence of B. infantis	978:1011	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	3	80	dep	6-	490:491	arg1	to					493:494	to	493:494	to	493:494	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	9	81	theme	B.	1030:1031	arg1	breve					1033:1037	B. breve	1030:1037	B. breve	1030:1037	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	5	82	from	analysis	627:634	arg1	metagenomes					656:666	gut metagenomes	652:666	gut metagenomes	652:666	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36794816	9	83	theme	lower	1065:1069	arg1	abundance					1071:1079	a lower abundance	1063:1079	a lower abundance of genes encoding pathogenic features	1063:1117	GMC types with a higher prevalence of B. infantis and abundance of B. breve also had a lower pH and a lower abundance of genes encoding pathogenic features.
36794816	10	84	dep	Se+	1329:1331	arg1	Le-					1334:1336	Le-	1334:1336	Le-	1334:1336	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	3	85	dep	composition	426:436	arg1	the					422:424	the	422:424	the	422:424	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	8	86	theme	gut	860:862	arg1	microbiome					864:873	the gut microbiome	856:873	the gut microbiome	856:873	Stratification of the gut microbiome into community (GMC) types revealed differences in composition and functional features.
36794816	6	87	dep	B.	813:814	arg1	longum					816:821	longum	816:821	longum	816:821	infantis (B. infantis) in Kenyan infants (80%), and possible co-existence of this subspecies with B. longum subsp.
36794816	10	88	from	enrichment	1398:1407	arg1	2'-fucosyllactose					1412:1428	2'-fucosyllactose	1412:1428	2'-fucosyllactose	1412:1428	An analysis of human milk oligosaccharides (HMOs) classified the human milk (HM) samples into four groups defined on the basis of secretor and Lewis polymorphisms revealed a higher prevalence of HM group III (Se+, Le-) (22%) than in most previously studied populations, with an enrichment in 2'-fucosyllactose.
36794816	8	89	from	differences	911:921	arg1	composition					926:936	composition	926:936	composition	926:936	Stratification of the gut microbiome into community (GMC) types revealed differences in composition and functional features.
36794816	8	89	from	differences	911:921	arg1	features					953:960	functional features	942:960	functional features	942:960	Stratification of the gut microbiome into community (GMC) types revealed differences in composition and functional features.
36794816	3	90	theme	gut	471:473	arg1	microbiomes					475:485	the gut microbiomes	467:485	the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105)	467:532	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	1	91	theme	key	228:230	arg1	role					232:235	a key role	226:235	a key role	226:235	The gut microbiota evolves rapidly after birth, responding dynamically to environmental factors and playing a key role in short- and long-term health.
36794816	12	92	from	variation	1769:1777	arg1	population					1798:1807	an understudied population	1782:1807	an understudied population with limited exposure to modern microbiome-altering factors	1782:1867	This study sheds light on gut microbiome variation in an understudied population with limited exposure to modern microbiome-altering factors.
36794816	11	93	theme	Kenyan	1495:1500	arg1	infants					1502:1508	partially breastfed Kenyan infants	1475:1508	partially breastfed Kenyan infants	1475:1508	Our results show that the gut microbiome of partially breastfed Kenyan infants over the age of six months is enriched in bacteria from the Bifidobacterium community, including B. infantis, and that the high prevalence of a specific HM group may indicate a specific HMO-gut microbiome association.
36794816	3	94	theme	Kenyan	509:514	arg1	n					525:525	n = 105	525:531	n = 105	525:531	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	3	94	theme	Kenyan	509:514	arg1	infants					516:522	6- to 11-month-old Kenyan infants	490:522	6- to 11-month-old Kenyan infants (n = 105)	490:532	We studied the composition, function and variability of the gut microbiomes of 6- to 11-month-old Kenyan infants (n = 105).
36794816	5	95	from	metagenomes	656:666	arg1	analysis					627:634	A pangenomic analysis	614:634	A pangenomic analysis of B. longum in gut metagenomes	614:666	A pangenomic analysis of B. longum in gut metagenomes revealed a high prevalence of B. longum subsp.
36070613	8	0	from	differentiation	1305:1319	arg1	layer					1384:1388	the lower layer	1374:1388	the lower layer	1374:1388	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	8	0	from	differentiation	1305:1319	arg1	layer					1334:1338	the upper layer	1324:1338	the upper layer	1324:1338	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	5	1	theme	situ</i>	754:761	arg1	layer					798:802	an <i>in situ</i> mineralized, osteoconductive lower layer	745:802	layer	798:802	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	7	2	theme	%	1222:1222	arg1	strength					1160:1167	a compressive strength	1146:1167	a compressive strength of up to 2.5 MPa	1146:1184	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	7	2	theme	%	1222:1222	arg1	strain					1204:1209	a compressive strain	1190:1209	a compressive strain of up to 40%	1190:1222	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	4	3	from	application	583:593	arg1	engineering					619:629	osteochondral tissue engineering	598:629	osteochondral tissue engineering	598:629	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	5	4	dep	strong	649:654	arg1	osteochondral-mimicking					667:689	osteochondral-mimicking	667:689	osteochondral-mimicking	667:689	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	2	5	theme	osteochondral	317:329	arg1	unit					331:334	the osteochondral unit	313:334	the osteochondral unit	313:334	Developing layered and multiphasic hydrogels to mimic the intrinsic hierarchical structure of the osteochondral unit is a promising strategy.
36070613	8	6	theme	chondrogenic	1292:1303	arg1	differentiation					1305:1319	chondrogenic differentiation	1292:1319	chondrogenic differentiation in the upper layer	1292:1338	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	7	7	dep	40	1220:1221	arg1	to					1217:1218	to	1217:1218	to	1217:1218	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	4	8	theme	insufficient	443:454	arg1	strength					467:474	insufficient mechanical strength	443:474	insufficient mechanical strength	443:474	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	7	9	from	layer	1131:1135	arg1	deposition					1069:1078	the homogeneous deposition	1053:1078	the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer	1053:1135	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	7	9	from	layer	1131:1135	arg1	network					1041:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	5	10	theme	basic	810:814	arg1	bFGF					842:845	bFGF	842:845	bFGF	842:845	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	10	theme	basic	810:814	arg1	factor					834:839	a basic fibroblast growth factor	808:839	a basic fibroblast growth factor (bFGF)	808:846	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	2	11	theme	promising	341:349	arg1	strategy					351:358	a promising strategy	339:358	a promising strategy	339:358	Developing layered and multiphasic hydrogels to mimic the intrinsic hierarchical structure of the osteochondral unit is a promising strategy.
36070613	8	12	theme	upper	1328:1332	arg1	layer					1334:1338	the upper layer	1324:1338	the upper layer	1324:1338	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	5	13	dep	-incorporated	847:859	arg1	chondrogenic					862:873	chondrogenic	862:873	chondrogenic	862:873	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	4	14	theme	damaged	515:521	arg1	cartilage					523:531	damaged cartilage	515:531	damaged cartilage	515:531	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	7	15	theme	MPa	1182:1184	arg1	strength					1160:1167	a compressive strength	1146:1167	a compressive strength of up to 2.5 MPa	1146:1184	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	7	15	theme	MPa	1182:1184	arg1	strain					1204:1209	a compressive strain	1190:1209	a compressive strain of up to 40%	1190:1222	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	5	16	theme	lower	792:796	arg1	layer					798:802	an <i>in situ</i> mineralized, osteoconductive lower layer	745:802	layer	798:802	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	8	17	from	differentiation	1355:1369	arg1	layer					1384:1388	the lower layer	1374:1388	the lower layer	1374:1388	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	8	17	from	differentiation	1355:1369	arg1	layer					1334:1338	the upper layer	1324:1338	the upper layer	1324:1338	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	4	18	theme	biological	488:497	arg1	cues					499:502	biological cues	488:502	biological cues	488:502	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	9	19	theme	osteochondral	1558:1570	arg1	outcome					1587:1593	an improved osteochondral reconstruction outcome	1546:1593	an improved osteochondral reconstruction outcome	1546:1593	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	1	20	theme	osteochondral	164:176	arg1	repair					185:190	full-thickness osteochondral defect repair	149:190	full-thickness osteochondral defect repair	149:190	Fabrication of a hydrogel scaffold for full-thickness osteochondral defect repair remains a grand challenge.
36070613	0	21	theme	Osteochondral	81:93	arg1	Repair					102:107	Full-Thickness Osteochondral Defect Repair	66:107	Full-Thickness Osteochondral Defect Repair	66:107	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	9	22	theme	vivo</i>	1397:1404	arg1	implantation					1406:1417	<i>In vivo</i> implantation	1391:1417	<i>In vivo</i> implantation	1391:1417	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	2	23	theme	hierarchical	287:298	arg1	structure					300:308	the intrinsic hierarchical structure	273:308	the intrinsic hierarchical structure of the osteochondral unit	273:334	Developing layered and multiphasic hydrogels to mimic the intrinsic hierarchical structure of the osteochondral unit is a promising strategy.
36070613	5	24	theme	inducing	875:882	arg1	layer					890:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	25	theme	functional	691:700	arg1	bilayer-gel					727:737	bilayer-gel	727:737	bilayer-gel	727:737	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	25	theme	functional	691:700	arg1	hydrogel					717:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel	647:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	647:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	4	26	theme	osteochondral	598:610	arg1	engineering					619:629	osteochondral tissue engineering	598:629	osteochondral tissue engineering	598:629	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	7	27	theme	hydroxyapatite	1083:1096	arg1	nanoparticles					1098:1110	hydroxyapatite nanoparticles	1083:1110	hydroxyapatite nanoparticles (HAp)	1083:1116	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	7	27	theme	hydroxyapatite	1083:1096	arg1	HAp					1113:1115	HAp	1113:1115	HAp	1113:1115	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	0	28	theme	Defect	95:100	arg1	Repair					102:107	Full-Thickness Osteochondral Defect Repair	66:107	Full-Thickness Osteochondral Defect Repair	66:107	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	10	29	theme	defect	1680:1685	arg1	repair					1687:1692	full-thickness osteochondral defect repair	1651:1692	full-thickness osteochondral defect repair	1651:1692	The present bilayer-gel thus shows great potential for full-thickness osteochondral defect repair.
36070613	0	30	theme	Biomimetic	15:24	arg1	Hydrogels					52:60	Biomimetic Functional Chitosan-Based Hydrogels	15:60	Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair	15:107	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	0	30	theme	Biomimetic	15:24	arg1	High-Strength					0:12	High-Strength	0:12	High-Strength	0:12	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	9	31	theme	cartilage	1486:1494	arg1	formation					1517:1525	hyaline cartilage and subchondral bone formation	1478:1525	hyaline cartilage and subchondral bone formation	1478:1525	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	1	32	theme	hydrogel	127:134	arg1	scaffold					136:143	a hydrogel scaffold	125:143	a hydrogel scaffold for full-thickness osteochondral defect repair	125:190	Fabrication of a hydrogel scaffold for full-thickness osteochondral defect repair remains a grand challenge.
36070613	10	33	theme	full-thickness	1651:1664	arg1	repair					1687:1692	full-thickness osteochondral defect repair	1651:1692	full-thickness osteochondral defect repair	1651:1692	The present bilayer-gel thus shows great potential for full-thickness osteochondral defect repair.
36070613	0	34	theme	Chitosan-Based	37:50	arg1	Hydrogels					52:60	Biomimetic Functional Chitosan-Based Hydrogels	15:60	Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair	15:107	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	0	34	theme	Chitosan-Based	37:50	arg1	High-Strength					0:12	High-Strength	0:12	High-Strength	0:12	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	9	35	theme	subchondral	1500:1510	arg1	bone					1512:1515	subchondral bone	1500:1515	subchondral bone	1500:1515	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	6	36	theme	gradient	961:968	arg1	structure					975:983	a depth-dependent gradient pore structure	943:983	a depth-dependent gradient pore structure	943:983	The obtained bilayer-gel showed a depth-dependent gradient pore structure and composition.
36070613	2	37	theme	multiphasic	242:252	arg1	hydrogels					254:262	layered and multiphasic hydrogels	230:262	layered and multiphasic hydrogels	230:262	Developing layered and multiphasic hydrogels to mimic the intrinsic hierarchical structure of the osteochondral unit is a promising strategy.
36070613	7	38	dep	2.5	1178:1180	arg1	to					1175:1176	to	1175:1176	to	1175:1176	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	2	39	theme	layered	230:236	arg1	hydrogels					254:262	layered and multiphasic hydrogels	230:262	layered and multiphasic hydrogels	230:262	Developing layered and multiphasic hydrogels to mimic the intrinsic hierarchical structure of the osteochondral unit is a promising strategy.
36070613	7	40	theme	hydrogel	1032:1039	arg1	network					1041:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	8	41	theme	<i>In	1225:1229	arg1	study					1241:1245	<i>In vitro</i> study	1225:1245	<i>In vitro</i> study	1225:1245	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	5	42	theme	<i>in	748:752	arg1	layer					798:802	an <i>in situ</i> mineralized, osteoconductive lower layer	745:802	layer	798:802	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	7	43	theme	double	1013:1018	arg1	network					1041:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	9	44	theme	improved	1549:1556	arg1	outcome					1587:1593	an improved osteochondral reconstruction outcome	1546:1593	an improved osteochondral reconstruction outcome	1546:1593	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	5	45	theme	mineralized	763:773	arg1	layer					798:802	an <i>in situ</i> mineralized, osteoconductive lower layer	745:802	layer	798:802	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	6	46	theme	obtained	915:922	arg1	bilayer-gel					924:934	The obtained bilayer-gel	911:934	The obtained bilayer-gel	911:934	The obtained bilayer-gel showed a depth-dependent gradient pore structure and composition.
36070613	9	47	theme	reconstruction	1572:1585	arg1	outcome					1587:1593	an improved osteochondral reconstruction outcome	1546:1593	an improved osteochondral reconstruction outcome	1546:1593	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	2	48	theme	unit	331:334	arg1	structure					300:308	the intrinsic hierarchical structure	273:308	the intrinsic hierarchical structure of the osteochondral unit	273:334	Developing layered and multiphasic hydrogels to mimic the intrinsic hierarchical structure of the osteochondral unit is a promising strategy.
36070613	1	49	theme	grand	202:206	arg1	challenge					208:216	a grand challenge	200:216	a grand challenge	200:216	Fabrication of a hydrogel scaffold for full-thickness osteochondral defect repair remains a grand challenge.
36070613	8	50	theme	osteogenic	1344:1353	arg1	differentiation					1355:1369	osteogenic differentiation	1344:1369	osteogenic differentiation in the lower layer	1344:1388	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	7	51	theme	compressive	1192:1202	arg1	strain					1204:1209	a compressive strain	1190:1209	a compressive strain of up to 40%	1190:1222	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	4	52	theme	mechanical	456:465	arg1	strength					467:474	insufficient mechanical strength	443:474	insufficient mechanical strength	443:474	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	8	53	theme	lower	1378:1382	arg1	layer					1384:1388	the lower layer	1374:1388	the lower layer	1374:1388	<i>In vitro</i> study showed that the bilayer-gel facilitates both chondrogenic differentiation in the upper layer and osteogenic differentiation in the lower layer.
36070613	5	54	theme	fibroblast	816:825	arg1	bFGF					842:845	bFGF	842:845	bFGF	842:845	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	54	theme	fibroblast	816:825	arg1	factor					834:839	a basic fibroblast growth factor	808:839	a basic fibroblast growth factor (bFGF)	808:846	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	2	55	theme	intrinsic	277:285	arg1	structure					300:308	the intrinsic hierarchical structure	273:308	the intrinsic hierarchical structure of the osteochondral unit	273:334	Developing layered and multiphasic hydrogels to mimic the intrinsic hierarchical structure of the osteochondral unit is a promising strategy.
36070613	5	56	with	hydrogel	717:724	arg1	layer					798:802	an <i>in situ</i> mineralized, osteoconductive lower layer	745:802	layer	798:802	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	56	with	hydrogel	717:724	arg1	layer					890:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	4	57	theme	subchondral	537:547	arg1	tissue					549:554	subchondral tissue	537:554	subchondral tissue	537:554	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	5	58	theme	osteoconductive	776:790	arg1	layer					798:802	an <i>in situ</i> mineralized, osteoconductive lower layer	745:802	layer	798:802	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	7	59	theme	compressive	1148:1158	arg1	strength					1160:1167	a compressive strength	1146:1167	a compressive strength of up to 2.5 MPa	1146:1184	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	1	60	theme	scaffold	136:143	arg1	Fabrication					110:120	Fabrication	110:120	Fabrication of a hydrogel scaffold for full-thickness osteochondral defect repair	110:190	Fabrication of a hydrogel scaffold for full-thickness osteochondral defect repair remains a grand challenge.
36070613	3	61	theme	Chitosan-based	361:374	arg1	hydrogels					376:384	Chitosan-based hydrogels	361:384	Chitosan-based hydrogels	361:384	Chitosan-based hydrogels are widely applied for biomedical applications.
36070613	4	62	theme	cues	499:502	arg1	tissue					549:554	subchondral tissue	537:554	subchondral tissue	537:554	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	4	62	theme	cues	499:502	arg1	lack					480:483	lack	480:483	lack	480:483	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	4	62	theme	cues	499:502	arg1	strength					467:474	insufficient mechanical strength	443:474	insufficient mechanical strength	443:474	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	5	63	theme	strong	649:654	arg1	bilayer-gel					727:737	bilayer-gel	727:737	bilayer-gel	727:737	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	63	theme	strong	649:654	arg1	hydrogel					717:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel	647:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	647:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	1	64	theme	full-thickness	149:162	arg1	repair					185:190	full-thickness osteochondral defect repair	149:190	full-thickness osteochondral defect repair	149:190	Fabrication of a hydrogel scaffold for full-thickness osteochondral defect repair remains a grand challenge.
36070613	5	65	theme	-incorporated	847:859	arg1	layer					890:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	9	66	theme	<i>In	1391:1395	arg1	implantation					1406:1417	<i>In vivo</i> implantation	1391:1417	<i>In vivo</i> implantation	1391:1417	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	7	67	theme	lower	1125:1129	arg1	layer					1131:1135	the lower layer	1121:1135	the lower layer	1121:1135	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	1	68	theme	defect	178:183	arg1	repair					185:190	full-thickness osteochondral defect repair	149:190	full-thickness osteochondral defect repair	149:190	Fabrication of a hydrogel scaffold for full-thickness osteochondral defect repair remains a grand challenge.
36070613	4	69	theme	tissue	612:617	arg1	engineering					619:629	osteochondral tissue engineering	598:629	osteochondral tissue engineering	598:629	However, insufficient mechanical strength and lack of biological cues to restore damaged cartilage and subchondral tissue significantly hinder their application in osteochondral tissue engineering.
36070613	10	70	theme	present	1600:1606	arg1	bilayer-gel					1608:1618	The present bilayer-gel	1596:1618	The present bilayer-gel	1596:1618	The present bilayer-gel thus shows great potential for full-thickness osteochondral defect repair.
36070613	5	71	theme	chitosan-based	702:715	arg1	bilayer-gel					727:737	bilayer-gel	727:737	bilayer-gel	727:737	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	71	theme	chitosan-based	702:715	arg1	hydrogel					717:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel	647:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	647:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	7	72	theme	nanoparticles	1098:1110	arg1	deposition					1069:1078	the homogeneous deposition	1053:1078	the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer	1053:1135	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	7	72	theme	nanoparticles	1098:1110	arg1	network					1041:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	3	73	theme	biomedical	409:418	arg1	applications					420:431	biomedical applications	409:431	biomedical applications	409:431	Chitosan-based hydrogels are widely applied for biomedical applications.
36070613	10	74	theme	great	1631:1635	arg1	potential					1637:1645	great potential	1631:1645	great potential for full-thickness osteochondral defect repair	1631:1692	The present bilayer-gel thus shows great potential for full-thickness osteochondral defect repair.
36070613	5	75	theme	tough	660:664	arg1	bilayer-gel					727:737	bilayer-gel	727:737	bilayer-gel	727:737	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	75	theme	tough	660:664	arg1	hydrogel					717:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel	647:724	a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	647:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	0	76	theme	Functional	26:35	arg1	Hydrogels					52:60	Biomimetic Functional Chitosan-Based Hydrogels	15:60	Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair	15:107	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	0	76	theme	Functional	26:35	arg1	High-Strength					0:12	High-Strength	0:12	High-Strength	0:12	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	9	77	theme	hyaline	1478:1484	arg1	cartilage					1486:1494	hyaline cartilage	1478:1494	hyaline cartilage	1478:1494	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	7	78	theme	homogeneous	1057:1067	arg1	deposition					1069:1078	the homogeneous deposition	1053:1078	the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer	1053:1135	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	10	79	theme	osteochondral	1666:1678	arg1	repair					1687:1692	full-thickness osteochondral defect repair	1651:1692	full-thickness osteochondral defect repair	1651:1692	The present bilayer-gel thus shows great potential for full-thickness osteochondral defect repair.
36070613	5	80	theme	upper	884:888	arg1	layer					890:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer	808:894	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	0	81	theme	Full-Thickness	66:79	arg1	Repair					102:107	Full-Thickness Osteochondral Defect Repair	66:107	Full-Thickness Osteochondral Defect Repair	66:107	High-Strength, Biomimetic Functional Chitosan-Based Hydrogels for Full-Thickness Osteochondral Defect Repair.
36070613	9	82	theme	bone	1512:1515	arg1	formation					1517:1525	hyaline cartilage and subchondral bone formation	1478:1525	hyaline cartilage and subchondral bone formation	1478:1525	<i>In vivo</i> implantation revealed that the bilayer-gel could simultaneously promote hyaline cartilage and subchondral bone formation, thus resulting in an improved osteochondral reconstruction outcome.
36070613	6	83	theme	pore	970:973	arg1	structure					975:983	a depth-dependent gradient pore structure	943:983	a depth-dependent gradient pore structure	943:983	The obtained bilayer-gel showed a depth-dependent gradient pore structure and composition.
36070613	5	84	theme	growth	827:832	arg1	bFGF					842:845	bFGF	842:845	bFGF	842:845	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	5	84	theme	growth	827:832	arg1	factor					834:839	a basic fibroblast growth factor	808:839	a basic fibroblast growth factor (bFGF)	808:846	In this study, a strong and tough, osteochondral-mimicking functional chitosan-based hydrogel (bilayer-gel) with an <i>in situ</i> mineralized, osteoconductive lower layer and a basic fibroblast growth factor (bFGF)-incorporated, chondrogenic inducing upper layer was developed.
36070613	7	85	link	crosslinked	1020:1030	arg1	network					1041:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	6	86	theme	depth-dependent	945:959	arg1	structure					975:983	a depth-dependent gradient pore structure	943:983	a depth-dependent gradient pore structure	943:983	The obtained bilayer-gel showed a depth-dependent gradient pore structure and composition.
36070613	7	87	theme	crosslinked	1020:1030	arg1	network					1041:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
36070613	7	88	theme	strong	1006:1011	arg1	network					1041:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network	1002:1047	The strong double crosslinked hydrogel network and the homogeneous deposition of hydroxyapatite nanoparticles (HAp) at the lower layer provided a compressive strength of up to 2.5 MPa and a compressive strain of up to 40%.
37053174	1	0	with	horses	231:236	arg1	typhlocolitis					243:255	typhlocolitis	243:255	typhlocolitis	243:255	Next generation sequencing has demonstrated that alpha diversity of the fecal microbiota is significantly altered in horses with typhlocolitis.
37053174	9	1	theme	Greater	1147:1153	arg1	richness					1165:1172	Greater bacterial richness	1147:1172	Greater bacterial richness	1147:1172	Greater bacterial richness was identified in sequenced cecal contents as compared to cultured plates (P < 0.05).
37053174	8	2	theme	dominant	1130:1137	arg1	Proteobacteria					1107:1120	Proteobacteria	1107:1120	Proteobacteria	1107:1120	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	8	2	theme	dominant	1130:1137	arg1	phylum					1139:1144	the dominant phylum	1126:1144	the dominant phylum	1126:1144	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	5	3	theme	brain	670:674	arg1	BHI					692:694	BHI	692:694	BHI	692:694	Another was serially diluted with brain heart infusion (BHI) and plated onto five different agar media.
37053174	5	3	theme	brain	670:674	arg1	infusion					682:689	brain heart infusion	670:689	brain heart infusion (BHI)	670:695	Another was serially diluted with brain heart infusion (BHI) and plated onto five different agar media.
37053174	11	4	theme	healthy	1531:1537	arg1	horses					1539:1544	healthy horses	1531:1544	healthy horses	1531:1544	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	3	5	theme	Cecal	538:542	arg1	content					544:550	Cecal content	538:550	Cecal content	538:550	Cecal content was collected after euthanasia.
37053174	8	6	theme	healthy	976:982	arg1	horses					997:1002	healthy and diseased horses	976:1002	healthy and diseased horses	976:1002	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	0	7	with	horses	87:92	arg1	typhlocolitis					99:111	typhlocolitis	99:111	typhlocolitis	99:111	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	2	8	theme	bacterial	306:314	arg1	composition					316:326	the bacterial composition	302:326	the bacterial composition of the cecum content of horses with and without typhlocolitis	302:388	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	1	9	theme	fecal	186:190	arg1	microbiota					192:201	the fecal microbiota	182:201	the fecal microbiota	182:201	Next generation sequencing has demonstrated that alpha diversity of the fecal microbiota is significantly altered in horses with typhlocolitis.
37053174	8	10	theme	diseased	988:995	arg1	horses					997:1002	healthy and diseased horses	976:1002	healthy and diseased horses	976:1002	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	11	11	theme	diarrheic	1502:1510	arg1	horses					1512:1517	diarrheic horses	1502:1517	diarrheic horses	1502:1517	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	4	12	used	used	599:602	arg2	aliquot					587:593	An aliquot	584:593	An aliquot	584:593	An aliquot was used for direct 16S gene sequencing.
37053174	9	13	theme	cultured	1232:1239	arg1	plates					1241:1246	cultured plates	1232:1246	cultured plates	1232:1246	Greater bacterial richness was identified in sequenced cecal contents as compared to cultured plates (P < 0.05).
37053174	1	14	theme	Next	114:117	arg1	sequencing					130:139	Next generation sequencing	114:139	Next generation sequencing	114:139	Next generation sequencing has demonstrated that alpha diversity of the fecal microbiota is significantly altered in horses with typhlocolitis.
37053174	12	15	from	horses	1644:1649	arg1	samples					1621:1627	2/6 samples	1617:1627	2/6 samples from diarrheic horses	1617:1649	A higher relative abundance of Fusobacteriota was found in 2/6 samples from diarrheic horses.
37053174	0	16	theme	content	57:63	arg1	sequencing					37:46	Direct and culture-enriched 16S rRNA sequencing	0:46	sequencing	37:46	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	0	16	theme	content	57:63	arg1	horses					87:92	horses	87:92	horses with typhlocolitis	87:111	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	2	17	with	horses	476:481	arg1	typhlocolitis					494:506	acute typhlocolitis	488:506	acute typhlocolitis	488:506	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	2	18	theme	acute	488:492	arg1	typhlocolitis					494:506	acute typhlocolitis	488:506	acute typhlocolitis	488:506	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	12	19	located	found	1608:1612	arg1	samples					1621:1627	2/6 samples	1617:1627	2/6 samples from diarrheic horses	1617:1649	A higher relative abundance of Fusobacteriota was found in 2/6 samples from diarrheic horses.
37053174	12	19	located	found	1608:1612	arg2	abundance					1576:1584	A higher relative abundance	1558:1584	A higher relative abundance of Fusobacteriota	1558:1602	A higher relative abundance of Fusobacteriota was found in 2/6 samples from diarrheic horses.
37053174	0	20	theme	healthy	68:74	arg1	horses					76:81	healthy horses	68:81	healthy horses	68:81	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	7	21	theme	MiSeq	939:943	arg1	platform					945:952	the Illumina MiSeq platform	926:952	the Illumina MiSeq platform	926:952	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	1	22	theme	alpha	163:167	arg1	diversity					169:177	alpha diversity	163:177	alpha diversity of the fecal microbiota	163:201	Next generation sequencing has demonstrated that alpha diversity of the fecal microbiota is significantly altered in horses with typhlocolitis.
37053174	10	23	theme	horses	1403:1408	arg1	microbiota					1389:1398	the gut microbiota	1381:1398	the gut microbiota of horses	1381:1408	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	11	24	theme	diversity	1470:1478	arg1	lower					1493:1497	lower	1493:1497	lower	1493:1497	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	11	24	theme	diversity	1470:1478	arg1	indices					1480:1486	the alpha diversity indices	1460:1486	the alpha diversity indices	1460:1486	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	5	25	theme	different	718:726	arg1	media					733:737	five different agar media	713:737	five different agar media	713:737	Another was serially diluted with brain heart infusion (BHI) and plated onto five different agar media.
37053174	10	26	theme	molecular	1277:1285	arg1	profiling					1287:1295	Culture-enriched molecular profiling	1260:1295	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing	1260:1334	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	2	27	theme	case-control	511:522	arg1	design					530:535	a case-control study design	509:535	a case-control study design	509:535	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	2	28	theme	direct	398:403	arg1	sequencing					435:444	direct and culture-enriched 16S gene sequencing	398:444	sequencing	435:444	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	2	29	theme	healthy	453:459	arg1	horses					461:466	six healthy horses	449:466	six healthy horses	449:466	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	0	30	theme	Direct	0:5	arg1	sequencing					37:46	Direct and culture-enriched 16S rRNA sequencing	0:46	sequencing	37:46	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	11	31	theme	16S	1436:1438	arg1	amplification					1445:1457	direct cecum content 16S gene amplification	1415:1457	direct cecum content 16S gene amplification	1415:1457	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	2	32	theme	gene	430:433	arg1	sequencing					435:444	direct and culture-enriched 16S gene sequencing	398:444	sequencing	435:444	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	0	33	theme	culture-enriched	11:26	arg1	sequencing					37:46	Direct and culture-enriched 16S rRNA sequencing	0:46	sequencing	37:46	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	8	34	theme	cultured	1054:1061	arg1	medias					1063:1068	all cultured medias	1050:1068	all cultured medias	1050:1068	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	11	35	theme	cecum	1422:1426	arg1	amplification					1445:1457	direct cecum content 16S gene amplification	1415:1457	direct cecum content 16S gene amplification	1415:1457	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	12	36	theme	Fusobacteriota	1589:1602	arg1	abundance					1576:1584	A higher relative abundance	1558:1584	A higher relative abundance of Fusobacteriota	1558:1602	A higher relative abundance of Fusobacteriota was found in 2/6 samples from diarrheic horses.
37053174	2	37	theme	horses	476:481	arg1	sequencing					435:444	direct and culture-enriched 16S gene sequencing	398:444	sequencing	435:444	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	10	38	theme	Culture-enriched	1260:1275	arg1	profiling					1287:1295	Culture-enriched molecular profiling	1260:1295	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing	1260:1334	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	2	39	theme	culture-enriched	409:424	arg1	sequencing					435:444	direct and culture-enriched 16S gene sequencing	398:444	sequencing	435:444	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	0	40	theme	rRNA	32:35	arg1	sequencing					37:46	Direct and culture-enriched 16S rRNA sequencing	0:46	sequencing	37:46	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	13	41	from	role	1656:1659	arg1	colitis					1689:1695	equine colitis	1682:1695	equine colitis	1682:1695	The role of Fusobacteriota in equine colitis deserves investigation.
37053174	6	42	theme	culture	744:750	arg1	medias					752:757	All culture medias	740:757	All culture medias	740:757	All culture medias, except for MacConkey, were incubated anaerobically.
37053174	2	43	theme	content	341:347	arg1	composition					316:326	the bacterial composition	302:326	the bacterial composition of the cecum content of horses with and without typhlocolitis	302:388	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	8	44	from	phyla	967:971	arg1	horses					997:1002	healthy and diseased horses	976:1002	healthy and diseased horses	976:1002	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	11	45	dep	lower	1493:1497	arg1	<					1549:1549	P < 0.05	1547:1554	P < 0.05	1547:1554	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	10	46	theme	microbiota	1389:1398	arg1	study					1372:1376	the study	1368:1376	the study of the gut microbiota of horses	1368:1408	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	11	47	theme	P	1547:1547	arg1	<					1549:1549	P < 0.05	1547:1554	P < 0.05	1547:1554	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	7	48	theme	16S	883:885	arg1	extraction					871:880	DNA extraction	867:880	DNA extraction	867:880	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	7	48	theme	16S	883:885	arg1	amplification					891:903	16S PCR amplification	883:903	16S PCR amplification	883:903	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	4	49	theme	16S	615:617	arg1	sequencing					624:633	direct 16S gene sequencing	608:633	direct 16S gene sequencing	608:633	An aliquot was used for direct 16S gene sequencing.
37053174	2	50	theme	horses	352:357	arg1	content					341:347	the cecum content	331:347	the cecum content of horses	331:357	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	8	51	theme	Predominant	955:965	arg1	phyla					967:971	Predominant phyla	955:971	Predominant phyla in healthy and diseased horses	955:1002	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	9	52	theme	bacterial	1155:1163	arg1	richness					1165:1172	Greater bacterial richness	1147:1172	Greater bacterial richness	1147:1172	Greater bacterial richness was identified in sequenced cecal contents as compared to cultured plates (P < 0.05).
37053174	10	53	theme	rRNA	1315:1318	arg1	sequencing					1325:1334	16S rRNA gene sequencing	1311:1334	16S rRNA gene sequencing	1311:1334	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	2	54	theme	study	280:284	arg1	objective					262:270	The objective	258:270	The objective of this study	258:284	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	10	55	theme	gut	1385:1387	arg1	microbiota					1389:1398	the gut microbiota	1381:1398	the gut microbiota of horses	1381:1408	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	9	56	theme	sequenced	1192:1200	arg1	contents					1208:1215	sequenced cecal contents	1192:1215	sequenced cecal contents	1192:1215	Greater bacterial richness was identified in sequenced cecal contents as compared to cultured plates (P < 0.05).
37053174	9	57	theme	cecal	1202:1206	arg1	contents					1208:1215	sequenced cecal contents	1192:1215	sequenced cecal contents	1192:1215	Greater bacterial richness was identified in sequenced cecal contents as compared to cultured plates (P < 0.05).
37053174	1	58	theme	microbiota	192:201	arg1	diversity					169:177	alpha diversity	163:177	alpha diversity of the fecal microbiota	163:201	Next generation sequencing has demonstrated that alpha diversity of the fecal microbiota is significantly altered in horses with typhlocolitis.
37053174	12	59	theme	higher	1560:1565	arg1	abundance					1576:1584	A higher relative abundance	1558:1584	A higher relative abundance of Fusobacteriota	1558:1602	A higher relative abundance of Fusobacteriota was found in 2/6 samples from diarrheic horses.
37053174	13	60	theme	Fusobacteriota	1664:1677	arg1	role					1656:1659	The role	1652:1659	The role of Fusobacteriota in equine colitis	1652:1695	The role of Fusobacteriota in equine colitis deserves investigation.
37053174	7	61	theme	Bacterial	812:820	arg1	colonies					822:829	Bacterial colonies	812:829	Bacterial colonies	812:829	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	13	62	theme	equine	1682:1687	arg1	colitis					1689:1695	equine colitis	1682:1695	equine colitis	1682:1695	The role of Fusobacteriota in equine colitis deserves investigation.
37053174	5	63	theme	heart	676:680	arg1	BHI					692:694	BHI	692:694	BHI	692:694	Another was serially diluted with brain heart infusion (BHI) and plated onto five different agar media.
37053174	5	63	theme	heart	676:680	arg1	infusion					682:689	brain heart infusion	670:689	brain heart infusion (BHI)	670:695	Another was serially diluted with brain heart infusion (BHI) and plated onto five different agar media.
37053174	8	64	from	Bacteroidetes	1033:1045	arg1	medias					1063:1068	all cultured medias	1050:1068	all cultured medias	1050:1068	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	1	65	theme	generation	119:128	arg1	sequencing					130:139	Next generation sequencing	114:139	Next generation sequencing	114:139	Next generation sequencing has demonstrated that alpha diversity of the fecal microbiota is significantly altered in horses with typhlocolitis.
37053174	11	66	theme	gene	1440:1443	arg1	amplification					1445:1457	direct cecum content 16S gene amplification	1415:1457	direct cecum content 16S gene amplification	1415:1457	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	9	67	theme	P	1249:1249	arg1	<					1251:1251	P < 0.05	1249:1256	P < 0.05	1249:1256	Greater bacterial richness was identified in sequenced cecal contents as compared to cultured plates (P < 0.05).
37053174	2	68	with	horses	461:466	arg1	typhlocolitis					494:506	acute typhlocolitis	488:506	acute typhlocolitis	488:506	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	9	69	dep	compared	1220:1227	arg1	<					1251:1251	P < 0.05	1249:1256	P < 0.05	1249:1256	Greater bacterial richness was identified in sequenced cecal contents as compared to cultured plates (P < 0.05).
37053174	0	70	theme	horses	76:81	arg1	content					57:63	cecal content	51:63	cecal content of healthy horses	51:81	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	2	71	dep	was	286:288	arg1	design					530:535	a case-control study design	509:535	a case-control study design	509:535	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	11	72	theme	alpha	1464:1468	arg1	lower					1493:1497	lower	1493:1497	lower	1493:1497	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	11	72	theme	alpha	1464:1468	arg1	indices					1480:1486	the alpha diversity indices	1460:1486	the alpha diversity indices	1460:1486	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	12	73	theme	diarrheic	1634:1642	arg1	horses					1644:1649	diarrheic horses	1634:1649	diarrheic horses	1634:1649	A higher relative abundance of Fusobacteriota was found in 2/6 samples from diarrheic horses.
37053174	2	74	theme	study	524:528	arg1	design					530:535	a case-control study design	509:535	a case-control study design	509:535	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	5	75	theme	agar	728:731	arg1	media					733:737	five different agar media	713:737	five different agar media	713:737	Another was serially diluted with brain heart infusion (BHI) and plated onto five different agar media.
37053174	7	76	theme	Illumina	930:937	arg1	platform					945:952	the Illumina MiSeq platform	926:952	the Illumina MiSeq platform	926:952	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	11	77	theme	content	1428:1434	arg1	amplification					1445:1457	direct cecum content 16S gene amplification	1415:1457	direct cecum content 16S gene amplification	1415:1457	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	0	78	theme	16S	28:30	arg1	sequencing					37:46	Direct and culture-enriched 16S rRNA sequencing	0:46	sequencing	37:46	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	12	79	theme	relative	1567:1574	arg1	abundance					1576:1584	A higher relative abundance	1558:1584	A higher relative abundance of Fusobacteriota	1558:1602	A higher relative abundance of Fusobacteriota was found in 2/6 samples from diarrheic horses.
37053174	11	80	theme	direct	1415:1420	arg1	amplification					1445:1457	direct cecum content 16S gene amplification	1415:1457	direct cecum content 16S gene amplification	1415:1457	For direct cecum content 16S gene amplification, the alpha diversity indices were lower in diarrheic horses compared to healthy horses (P < 0.05).
37053174	2	81	theme	16S	426:428	arg1	sequencing					435:444	direct and culture-enriched 16S gene sequencing	398:444	sequencing	435:444	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	10	82	theme	gene	1320:1323	arg1	sequencing					1325:1334	16S rRNA gene sequencing	1311:1334	16S rRNA gene sequencing	1311:1334	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	0	83	theme	cecal	51:55	arg1	content					57:63	cecal content	51:63	cecal content of healthy horses	51:81	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	8	84	theme	MacConkey	1082:1090	arg1	agar					1092:1095	MacConkey agar	1082:1095	MacConkey agar	1082:1095	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	2	85	theme	horses	461:466	arg1	sequencing					435:444	direct and culture-enriched 16S gene sequencing	398:444	sequencing	435:444	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	10	86	theme	16S	1311:1313	arg1	sequencing					1325:1334	16S rRNA gene sequencing	1311:1334	16S rRNA gene sequencing	1311:1334	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	2	87	theme	cecum	335:339	arg1	content					341:347	the cecum content	331:347	the cecum content of horses	331:357	The objective of this study was to evaluate the bacterial composition of the cecum content of horses with and without typhlocolitis through direct and culture-enriched 16S gene sequencing of six healthy horses and six horses with acute typhlocolitis; a case-control study design.
37053174	7	88	theme	PCR	887:889	arg1	extraction					871:880	DNA extraction	867:880	DNA extraction	867:880	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	7	88	theme	PCR	887:889	arg1	amplification					891:903	16S PCR amplification	883:903	16S PCR amplification	883:903	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	8	89	from	phylum	1139:1144	arg1	agar					1092:1095	MacConkey agar	1082:1095	MacConkey agar	1082:1095	Predominant phyla in healthy and diseased horses were Firmicutes, followed by Bacteroidetes in all cultured medias, except for MacConkey agar, in which Proteobacteria was the dominant phylum.
37053174	4	90	theme	direct	608:613	arg1	sequencing					624:633	direct 16S gene sequencing	608:633	direct 16S gene sequencing	608:633	An aliquot was used for direct 16S gene sequencing.
37053174	10	91	theme	alternative	1345:1355	arg1	method					1357:1362	an alternative method	1342:1362	an alternative method for the study of the gut microbiota of horses	1342:1408	Culture-enriched molecular profiling combined with 16S rRNA gene sequencing offer an alternative method for the study of the gut microbiota of horses.
37053174	7	92	used	used	858:861	arg2	colonies					822:829	Bacterial colonies	812:829	Bacterial colonies	812:829	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	4	93	theme	gene	619:622	arg1	sequencing					624:633	direct 16S gene sequencing	608:633	direct 16S gene sequencing	608:633	An aliquot was used for direct 16S gene sequencing.
37053174	0	94	with	sequencing	37:46	arg1	typhlocolitis					99:111	typhlocolitis	99:111	typhlocolitis	99:111	Direct and culture-enriched 16S rRNA sequencing of cecal content of healthy horses and horses with typhlocolitis.
37053174	7	95	theme	DNA	867:869	arg1	extraction					871:880	DNA extraction	867:880	DNA extraction	867:880	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
37053174	7	95	theme	DNA	867:869	arg1	amplification					891:903	16S PCR amplification	883:903	16S PCR amplification	883:903	Bacterial colonies were harvested in bulk and used for DNA extraction, 16S PCR amplification, and sequenced using the Illumina MiSeq platform.
35138894	5	0	theme	binding	787:793	arg1	assays					795:800	binding assays	787:800	binding assays	787:800	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	7	1	theme	autoimmune	1109:1118	arg1	disease					1120:1126	a major human autoimmune disease	1095:1126	a major human autoimmune disease	1095:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	2	2	gly	glycosylation	316:328	arg2	sites					330:334	N-linked glycosylation sites	307:334	N-linked glycosylation sites	307:334	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	7	3	theme	B	1084:1084	arg1	cells					1086:1090	autoreactive B cells	1071:1090	autoreactive B cells in a major human autoimmune disease	1071:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	7	4	theme	cells	1086:1090	arg1	tolerance					1058:1066	tolerance	1058:1066	tolerance of autoreactive B cells in a major human autoimmune disease	1058:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	1	5	theme	rheumatoid	164:173	arg1	arthritis					175:183	rheumatoid arthritis	164:183	rheumatoid arthritis	164:183	The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs).
35138894	4	6	theme	key	601:603	arg1	characteristics					605:619	key characteristics	601:619	key characteristics of VDGs on human B cell biology	601:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	0	7	theme	human	95:99	arg1	activation					121:130	human autoreactive B cell activation	95:130	human autoreactive B cell activation	95:130	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	7	8	theme	major	1097:1101	arg1	disease					1120:1126	a major human autoimmune disease	1095:1126	a major human autoimmune disease	1095:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	4	9	theme	VDGs	624:627	arg1	characteristics					605:619	key characteristics	601:619	key characteristics of VDGs on human B cell biology	601:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	7	10	theme	human	1103:1107	arg1	disease					1120:1126	a major human autoimmune disease	1095:1126	a major human autoimmune disease	1095:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	0	11	theme	B	114:114	arg1	activation					121:130	human autoreactive B cell activation	95:130	human autoreactive B cell activation	95:130	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	6	12	theme	cell	905:908	arg1	surface					910:916	the B cell surface	899:916	the B cell surface	899:916	We found that VDG-expressing B cell receptors stay longer on the B cell surface and that VDGs enhance B cell activation.
35138894	1	13	from	autoantibodies	146:159	arg1	arthritis					175:183	rheumatoid arthritis	164:183	rheumatoid arthritis	164:183	The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs).
35138894	2	14	theme	selective	281:289	arg1	introduction					291:302	the selective introduction	277:302	the selective introduction of N-linked glycosylation sites during somatic hypermutation	277:363	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	3	15	theme	VDGs	464:467	arg1	consequences					448:459	the functional consequences	433:459	the functional consequences of VDGs on autoreactive B cells	433:491	However, the functional consequences of VDGs on autoreactive B cells remain elusive.
35138894	0	16	theme	autoreactive	101:112	arg1	activation					121:130	human autoreactive B cell activation	95:130	human autoreactive B cell activation	95:130	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	6	17	theme	B	903:903	arg1	surface					910:916	the B cell surface	899:916	the B cell surface	899:916	We found that VDG-expressing B cell receptors stay longer on the B cell surface and that VDGs enhance B cell activation.
35138894	7	18	theme	autoreactive	1071:1082	arg1	cells					1086:1090	autoreactive B cells	1071:1090	autoreactive B cells in a major human autoimmune disease	1071:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	4	19	from	characteristics	605:619	arg1	biology					645:651	human B cell biology	632:651	human B cell biology	632:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	0	20	theme	Ig	8:9	arg1	glycosylation					27:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	5	21	theme	Crystal	654:660	arg1	structures					662:671	Crystal structures	654:671	Crystal structures	654:671	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	2	22	theme	disease	403:409	arg1	development					411:421	disease development	403:421	disease development	403:421	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	0	23	theme	Surface	0:6	arg1	glycosylation					27:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	6	24	theme	B	940:940	arg1	activation					947:956	B cell activation	940:956	B cell activation	940:956	We found that VDG-expressing B cell receptors stay longer on the B cell surface and that VDGs enhance B cell activation.
35138894	2	25	theme	sites	330:334	arg1	introduction					291:302	the selective introduction	277:302	the selective introduction of N-linked glycosylation sites during somatic hypermutation	277:363	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	4	26	from	VDGs	624:627	arg1	biology					645:651	human B cell biology	632:651	human B cell biology	632:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	0	27	theme	domain	20:25	arg1	glycosylation					27:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	7	28	from	cells	1086:1090	arg1	disease					1120:1126	a major human autoimmune disease	1095:1126	a major human autoimmune disease	1095:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	2	29	theme	glycosylation	316:328	arg1	sites					330:334	N-linked glycosylation sites	307:334	N-linked glycosylation sites	307:334	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	1	30	theme	variable	206:213	arg1	VDGs					231:234	VDGs	231:234	VDGs	231:234	The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs).
35138894	1	30	theme	variable	206:213	arg1	glycans					222:228	variable domain glycans	206:228	variable domain glycans (VDGs)	206:235	The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs).
35138894	0	31	theme	variable	11:18	arg1	glycosylation					27:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation	0:39	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	0	32	theme	cell	116:119	arg1	activation					121:130	human autoreactive B cell activation	95:130	human autoreactive B cell activation	95:130	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	2	33	theme	N-linked	307:314	arg1	sites					330:334	N-linked glycosylation sites	307:334	N-linked glycosylation sites	307:334	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	1	34	theme	domain	215:220	arg1	VDGs					231:234	VDGs	231:234	VDGs	231:234	The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs).
35138894	1	34	theme	domain	215:220	arg1	glycans					222:228	variable domain glycans	206:228	variable domain glycans (VDGs)	206:235	The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs).
35138894	5	35	theme	antigen-binding	728:742	arg1	pocket					744:749	the antigen-binding pocket	724:749	the antigen-binding pocket	724:749	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	4	36	from	biology	645:651	arg1	characteristics					605:619	key characteristics	601:619	key characteristics of VDGs on human B cell biology	601:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	3	37	theme	autoreactive	472:483	arg1	cells					487:491	autoreactive B cells	472:491	autoreactive B cells	472:491	However, the functional consequences of VDGs on autoreactive B cells remain elusive.
35138894	3	38	theme	functional	437:446	arg1	consequences					448:459	the functional consequences	433:459	the functional consequences of VDGs on autoreactive B cells	433:491	However, the functional consequences of VDGs on autoreactive B cells remain elusive.
35138894	4	39	theme	B	565:565	arg1	assays					572:577	functional B cell assays	554:577	functional B cell assays	554:577	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	5	40	theme	pocket	744:749	arg1	vicinity					712:719	the vicinity	708:719	the vicinity of the antigen-binding pocket	708:749	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	5	41	from	positioned	694:703	arg1	vicinity					712:719	the vicinity	708:719	the vicinity of the antigen-binding pocket	708:749	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	7	42	theme	VDGs	1019:1022	arg1	acquisition					1004:1014	the acquisition	1000:1014	the acquisition of VDGs	1000:1022	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	3	43	theme	B	485:485	arg1	cells					487:491	autoreactive B cells	472:491	autoreactive B cells	472:491	However, the functional consequences of VDGs on autoreactive B cells remain elusive.
35138894	2	44	link	N-linked	307:314	arg1	sites					330:334	N-linked glycosylation sites	307:334	N-linked glycosylation sites	307:334	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	7	45	from	disease	1120:1126	arg1	tolerance					1058:1066	tolerance	1058:1066	tolerance of autoreactive B cells in a major human autoimmune disease	1058:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	7	46	theme	tolerance	1058:1066	arg1	breach					1048:1053	the breach	1044:1053	the breach of tolerance of autoreactive B cells in a major human autoimmune disease	1044:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	0	47	theme	autoantigen	49:59	arg1	binding					61:67	autoantigen binding	49:67	autoantigen binding	49:67	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	6	48	theme	cell	942:945	arg1	activation					947:956	B cell activation	940:956	B cell activation	940:956	We found that VDG-expressing B cell receptors stay longer on the B cell surface and that VDGs enhance B cell activation.
35138894	6	49	theme	cell	869:872	arg1	receptors					874:882	VDG-expressing B cell receptors	852:882	VDG-expressing B cell receptors	852:882	We found that VDG-expressing B cell receptors stay longer on the B cell surface and that VDGs enhance B cell activation.
35138894	4	50	theme	cell	567:570	arg1	assays					572:577	functional B cell assays	554:577	functional B cell assays	554:577	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	6	51	theme	B	867:867	arg1	receptors					874:882	VDG-expressing B cell receptors	852:882	VDG-expressing B cell receptors	852:882	We found that VDG-expressing B cell receptors stay longer on the B cell surface and that VDGs enhance B cell activation.
35138894	0	52	gly	glycosylation	27:39	arg1	autoantigen					49:59	autoantigen binding	49:67	autoantigen binding	49:67	Surface Ig variable domain glycosylation affects autoantigen binding and acts as threshold for human autoreactive B cell activation.
35138894	4	53	theme	human	632:636	arg1	biology					645:651	human B cell biology	632:651	human B cell biology	632:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	6	54	theme	VDG-expressing	852:865	arg1	receptors					874:882	VDG-expressing B cell receptors	852:882	VDG-expressing B cell receptors	852:882	We found that VDG-expressing B cell receptors stay longer on the B cell surface and that VDGs enhance B cell activation.
35138894	3	55	from	consequences	448:459	arg1	cells					487:491	autoreactive B cells	472:491	autoreactive B cells	472:491	However, the functional consequences of VDGs on autoreactive B cells remain elusive.
35138894	2	56	theme	somatic	343:349	arg1	hypermutation					351:363	somatic hypermutation	343:363	somatic hypermutation	343:363	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	5	57	from	vicinity	712:719	arg1	positioned					694:703	positioned	694:703	positioned	694:703	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	4	58	theme	functional	554:563	arg1	assays					572:577	functional B cell assays	554:577	functional B cell assays	554:577	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	5	59	theme	dynamic	756:762	arg1	modeling					764:771	dynamic modeling	756:771	dynamic modeling combined with binding assays	756:800	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	7	60	from	tolerance	1058:1066	arg1	disease					1120:1126	a major human autoimmune disease	1095:1126	a major human autoimmune disease	1095:1126	These results provide a rationale on how the acquisition of VDGs might contribute to the breach of tolerance of autoreactive B cells in a major human autoimmune disease.
35138894	1	61	theme	hallmark	137:144	arg1	autoantibodies					146:159	The hallmark autoantibodies	133:159	The hallmark autoantibodies in rheumatoid arthritis	133:183	The hallmark autoantibodies in rheumatoid arthritis are characterized by variable domain glycans (VDGs).
35138894	4	62	theme	cell	640:643	arg1	biology					645:651	human B cell biology	632:651	human B cell biology	632:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
35138894	5	63	from	impact	819:824	arg1	binding					829:835	binding	829:835	binding	829:835	Crystal structures showed that VDGs are positioned in the vicinity of the antigen-binding pocket, and dynamic modeling combined with binding assays elucidated their impact on binding.
35138894	2	64	theme	abundant	244:251	arg1	occurrence					253:262	Their abundant occurrence	238:262	Their abundant occurrence	238:262	Their abundant occurrence results from the selective introduction of N-linked glycosylation sites during somatic hypermutation, and their presence is predictive for disease development.
35138894	4	65	theme	B	638:638	arg1	biology					645:651	human B cell biology	632:651	human B cell biology	632:651	Combining crystallography, glycobiology, and functional B cell assays allowed us to dissect key characteristics of VDGs on human B cell biology.
36880221	8	0	theme	More	1109:1112	arg1	counts					1129:1134	More S. Typhimurium counts	1109:1134	More S. Typhimurium counts in feces and other tissues	1109:1161	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	6	1	theme	normal	908:913	arg1	mice					924:927	normal diet-fed mice	908:927	normal diet-fed mice	908:927	Bacteroidetes and Verrucomicrobiota were more abundant in normal diet-fed mice than in HSD-fed mice.
36880221	7	2	theme	fatty	973:977	arg1	acids					979:983	short-chain fatty acids	961:983	short-chain fatty acids (SCFAs)	961:991	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	7	2	theme	fatty	973:977	arg1	SCFAs					986:990	SCFAs	986:990	SCFAs	986:990	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	2	3	theme	various	362:368	arg1	diseases					370:377	various diseases	362:377	various diseases	362:377	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	10	4	theme	fecal	1485:1489	arg1	microbiota					1491:1500	HSD fecal microbiota	1481:1500	HSD fecal microbiota	1481:1500	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	10	5	theme	Typhimurium	1428:1438	arg1	burdens					1440:1446	lower Salmonella Typhimurium burdens	1411:1446	lower Salmonella Typhimurium burdens	1411:1446	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	1	6	theme	high	169:172	arg1	fat					174:176	high fat	169:176	high fat	169:176	A westernized diet characterized by high fat and sugar is tightly associated with the development of metabolic diseases and inflammatory bowel disease.
36880221	1	7	theme	inflammatory	257:268	arg1	disease					276:282	inflammatory bowel disease	257:282	inflammatory bowel disease	257:282	A westernized diet characterized by high fat and sugar is tightly associated with the development of metabolic diseases and inflammatory bowel disease.
36880221	8	8	theme	Typhimurium	1117:1127	arg1	counts					1129:1134	More S. Typhimurium counts	1109:1134	More S. Typhimurium counts in feces and other tissues	1109:1161	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	7	9	theme	control	1075:1081	arg1	group					1083:1087	the control group	1071:1087	the control group	1071:1087	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	2	10	theme	high-fat	296:303	arg1	diet					305:308	a high-fat diet	294:308	a high-fat diet	294:308	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	3	11	from	effect	545:550	arg1	infection					609:617	Salmonella Typhimurium-induced infection	578:617	Salmonella Typhimurium-induced infection	578:617	This study aimed to explore the effect of a high sucrose diet on Salmonella Typhimurium-induced infection.
36880221	1	12	theme	bowel	270:274	arg1	disease					276:282	inflammatory bowel disease	257:282	inflammatory bowel disease	257:282	A westernized diet characterized by high fat and sugar is tightly associated with the development of metabolic diseases and inflammatory bowel disease.
36880221	0	13	theme	mice	91:94	arg1	susceptibility					73:86	the susceptibility	69:86	the susceptibility of mice to Salmonella Typhimurium infection	69:130	A high-sucrose diet causes microbiota composition shift and promotes the susceptibility of mice to Salmonella Typhimurium infection.
36880221	10	14	theme	microbiota	1313:1322	arg1	FMT					1341:1343	FMT	1341:1343	FMT	1341:1343	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	10	14	theme	microbiota	1313:1322	arg1	transplantation					1324:1338	Fecal microbiota transplantation	1307:1338	Fecal microbiota transplantation (FMT)	1307:1344	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	2	15	from	involvement	347:357	arg1	diseases					370:377	various diseases	362:377	various diseases	362:377	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	1	16	theme	disease	276:282	arg1	development					219:229	the development	215:229	the development of metabolic diseases and inflammatory bowel disease	215:282	A westernized diet characterized by high fat and sugar is tightly associated with the development of metabolic diseases and inflammatory bowel disease.
36880221	0	17	theme	Typhimurium	110:120	arg1	infection					122:130	Salmonella Typhimurium infection	99:130	Salmonella Typhimurium infection	99:130	A high-sucrose diet causes microbiota composition shift and promotes the susceptibility of mice to Salmonella Typhimurium infection.
36880221	11	18	theme	intestinal	1679:1688	arg1	homeostasis					1690:1700	intestinal homeostasis	1679:1700	intestinal homeostasis	1679:1700	Together, these findings suggest that the excessive intake of sucrose disturbs intestinal homeostasis and predisposes mice to Salmonella-induced infection.
36880221	8	19	theme	other	1149:1153	arg1	tissues					1155:1161	other tissues	1149:1161	other tissues	1149:1161	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	5	20	theme	microbial	834:842	arg1	taxa					844:847	certain microbial taxa	826:847	certain microbial taxa	826:847	The high-sugar diet profoundly altered the relative abundance of certain microbial taxa.
36880221	0	21	theme	Salmonella	99:108	arg1	infection					122:130	Salmonella Typhimurium infection	99:130	Salmonella Typhimurium infection	99:130	A high-sucrose diet causes microbiota composition shift and promotes the susceptibility of mice to Salmonella Typhimurium infection.
36880221	9	22	theme	HSD-fed	1293:1299	arg1	mice					1301:1304	HSD-fed mice	1293:1304	HSD-fed mice	1293:1304	Tight junction proteins and antimicrobial peptides were significantly decreased in HSD-fed mice.
36880221	0	23	theme	composition	38:48	arg1	shift					50:54	microbiota composition shift	27:54	microbiota composition shift	27:54	A high-sucrose diet causes microbiota composition shift and promotes the susceptibility of mice to Salmonella Typhimurium infection.
36880221	5	24	theme	taxa	844:847	arg1	abundance					813:821	the relative abundance	800:821	the relative abundance of certain microbial taxa	800:847	The high-sugar diet profoundly altered the relative abundance of certain microbial taxa.
36880221	0	25	theme	high-sucrose	2:13	arg1	diet					15:18	A high-sucrose diet	0:18	A high-sucrose diet	0:18	A high-sucrose diet causes microbiota composition shift and promotes the susceptibility of mice to Salmonella Typhimurium infection.
36880221	10	26	theme	altered	1523:1529	arg1	communities					1541:1551	the altered microbial communities	1519:1551	the altered microbial communities	1519:1551	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	10	27	theme	lower	1411:1415	arg1	burdens					1440:1446	lower Salmonella Typhimurium burdens	1411:1446	lower Salmonella Typhimurium burdens	1411:1446	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	3	28	theme	high	557:560	arg1	diet					570:573	a high sucrose diet	555:573	a high sucrose diet	555:573	This study aimed to explore the effect of a high sucrose diet on Salmonella Typhimurium-induced infection.
36880221	10	29	theme	infection	1589:1597	arg1	severity					1577:1584	the severity	1573:1584	the severity of infection	1573:1597	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	11	30	theme	Salmonella-induced	1726:1743	arg1	infection					1745:1753	Salmonella-induced infection	1726:1753	Salmonella-induced infection	1726:1753	Together, these findings suggest that the excessive intake of sucrose disturbs intestinal homeostasis and predisposes mice to Salmonella-induced infection.
36880221	10	31	theme	fecal	1390:1394	arg1	microbiota					1396:1405	normal fecal microbiota	1383:1405	normal fecal microbiota	1383:1405	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	3	32	theme	sucrose	562:568	arg1	diet					570:573	a high sucrose diet	555:573	a high sucrose diet	555:573	This study aimed to explore the effect of a high sucrose diet on Salmonella Typhimurium-induced infection.
36880221	2	33	theme	fewer	380:384	arg1	studies					386:392	fewer studies	380:392	fewer studies	380:392	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	2	34	theme	diet	435:438	arg1	impact					412:417	the impact	408:417	the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections	408:510	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	11	35	theme	sucrose	1662:1668	arg1	intake					1652:1657	the excessive intake	1638:1657	the excessive intake of sucrose	1638:1668	Together, these findings suggest that the excessive intake of sucrose disturbs intestinal homeostasis and predisposes mice to Salmonella-induced infection.
36880221	10	36	theme	normal	1383:1388	arg1	microbiota					1396:1405	normal fecal microbiota	1383:1405	normal fecal microbiota	1383:1405	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	5	37	theme	relative	804:811	arg1	abundance					813:821	the relative abundance	800:821	the relative abundance of certain microbial taxa	800:847	The high-sugar diet profoundly altered the relative abundance of certain microbial taxa.
36880221	2	38	theme	high-sugar	424:433	arg1	diet					435:438	a high-sugar diet	422:438	a high-sugar diet	422:438	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	6	39	from	mice	945:948	arg1	abundant					896:903	abundant	896:903	abundant	896:903	Bacteroidetes and Verrucomicrobiota were more abundant in normal diet-fed mice than in HSD-fed mice.
36880221	4	40	theme	sucrose	676:682	arg1	HSD					690:692	HSD	690:692	HSD	690:692	C57BL/6 mice received a normal diet (Control) or a high sucrose diet (HSD) for eight weeks and then were infected by Salmonella Typhimurium.
36880221	4	40	theme	sucrose	676:682	arg1	diet					684:687	a high sucrose diet	669:687	a high sucrose diet (HSD)	669:693	C57BL/6 mice received a normal diet (Control) or a high sucrose diet (HSD) for eight weeks and then were infected by Salmonella Typhimurium.
36880221	10	41	theme	microbial	1531:1539	arg1	communities					1541:1551	the altered microbial communities	1519:1551	the altered microbial communities	1519:1551	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	9	42	theme	antimicrobial	1238:1250	arg1	peptides					1252:1259	antimicrobial peptides	1238:1259	antimicrobial peptides	1238:1259	Tight junction proteins and antimicrobial peptides were significantly decreased in HSD-fed mice.
36880221	2	43	theme	diseases	470:477	arg1	development					447:457	the development	443:457	the development of certain diseases, particularly enteric infections	443:510	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	11	44	theme	excessive	1642:1650	arg1	intake					1652:1657	the excessive intake	1638:1657	the excessive intake of sucrose	1638:1668	Together, these findings suggest that the excessive intake of sucrose disturbs intestinal homeostasis and predisposes mice to Salmonella-induced infection.
36880221	10	45	theme	HSD	1481:1483	arg1	microbiota					1491:1500	HSD fecal microbiota	1481:1500	HSD fecal microbiota	1481:1500	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	3	46	theme	Salmonella	578:587	arg1	infection					609:617	Salmonella Typhimurium-induced infection	578:617	Salmonella Typhimurium-induced infection	578:617	This study aimed to explore the effect of a high sucrose diet on Salmonella Typhimurium-induced infection.
36880221	0	47	theme	microbiota	27:36	arg1	composition					38:48	microbiota composition	27:48	microbiota composition shift	27:54	A high-sucrose diet causes microbiota composition shift and promotes the susceptibility of mice to Salmonella Typhimurium infection.
36880221	6	48	theme	HSD-fed	937:943	arg1	mice					945:948	HSD-fed mice	937:948	HSD-fed mice	937:948	Bacteroidetes and Verrucomicrobiota were more abundant in normal diet-fed mice than in HSD-fed mice.
36880221	2	49	theme	certain	462:468	arg1	infections					501:510	enteric infections	493:510	enteric infections	493:510	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	2	49	theme	certain	462:468	arg1	diseases					470:477	certain diseases	462:477	certain diseases	462:477	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	10	50	contain	had	1407:1409	arg2	burdens					1440:1446	lower Salmonella Typhimurium burdens	1411:1446	lower Salmonella Typhimurium burdens	1411:1446	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	10	50	contain	had	1407:1409	arg1	mice					1364:1367	mice	1364:1367	mice that received normal fecal microbiota	1364:1405	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	3	51	theme	Typhimurium-induced	589:607	arg1	infection					609:617	Salmonella Typhimurium-induced infection	578:617	Salmonella Typhimurium-induced infection	578:617	This study aimed to explore the effect of a high sucrose diet on Salmonella Typhimurium-induced infection.
36880221	4	52	theme	normal	644:649	arg1	Control					657:663	Control	657:663	Control	657:663	C57BL/6 mice received a normal diet (Control) or a high sucrose diet (HSD) for eight weeks and then were infected by Salmonella Typhimurium.
36880221	4	52	theme	normal	644:649	arg1	diet					651:654	a normal diet	642:654	a normal diet (Control)	642:664	C57BL/6 mice received a normal diet (Control) or a high sucrose diet (HSD) for eight weeks and then were infected by Salmonella Typhimurium.
36880221	7	53	theme	HSD	1098:1100	arg1	group					1102:1106	the HSD group	1094:1106	the HSD group	1094:1106	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	7	54	from	group	1083:1087	arg1	mice					1061:1064	mice	1061:1064	mice from the control group	1061:1087	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	6	55	from	abundant	896:903	arg1	mice					924:927	normal diet-fed mice	908:927	normal diet-fed mice	908:927	Bacteroidetes and Verrucomicrobiota were more abundant in normal diet-fed mice than in HSD-fed mice.
36880221	6	55	from	abundant	896:903	arg1	mice					945:948	HSD-fed mice	937:948	HSD-fed mice	937:948	Bacteroidetes and Verrucomicrobiota were more abundant in normal diet-fed mice than in HSD-fed mice.
36880221	3	56	theme	diet	570:573	arg1	effect					545:550	the effect	541:550	the effect of a high sucrose diet on Salmonella Typhimurium-induced infection	541:617	This study aimed to explore the effect of a high sucrose diet on Salmonella Typhimurium-induced infection.
36880221	7	57	theme	branched-chain	997:1010	arg1	BCFAs					1025:1029	BCFAs	1025:1029	BCFAs	1025:1029	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	7	57	theme	branched-chain	997:1010	arg1	acids					1018:1022	branched-chain fatty acids	997:1022	branched-chain fatty acids (BCFAs)	997:1030	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	5	58	theme	high-sugar	765:774	arg1	diet					776:779	The high-sugar diet	761:779	The high-sugar diet	761:779	The high-sugar diet profoundly altered the relative abundance of certain microbial taxa.
36880221	6	59	from	mice	924:927	arg1	abundant					896:903	abundant	896:903	abundant	896:903	Bacteroidetes and Verrucomicrobiota were more abundant in normal diet-fed mice than in HSD-fed mice.
36880221	9	60	theme	Tight	1210:1214	arg1	proteins					1225:1232	Tight junction proteins	1210:1232	Tight junction proteins	1210:1232	Tight junction proteins and antimicrobial peptides were significantly decreased in HSD-fed mice.
36880221	8	61	from	counts	1129:1134	arg1	feces					1139:1143	feces	1139:1143	feces	1139:1143	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	8	61	from	counts	1129:1134	arg1	tissues					1155:1161	other tissues	1149:1161	other tissues	1149:1161	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	10	62	theme	Fecal	1307:1311	arg1	FMT					1341:1343	FMT	1341:1343	FMT	1341:1343	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	10	62	theme	Fecal	1307:1311	arg1	transplantation					1324:1338	Fecal microbiota transplantation	1307:1338	Fecal microbiota transplantation (FMT)	1307:1344	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	10	63	theme	Salmonella	1417:1426	arg1	burdens					1440:1446	lower Salmonella Typhimurium burdens	1411:1446	lower Salmonella Typhimurium burdens	1411:1446	Fecal microbiota transplantation (FMT) demonstrated that mice that received normal fecal microbiota had lower Salmonella Typhimurium burdens compared with mice that received HSD fecal microbiota, indicating that the altered microbial communities are associated with the severity of infection.
36880221	7	64	theme	fatty	1012:1016	arg1	BCFAs					1025:1029	BCFAs	1025:1029	BCFAs	1025:1029	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	7	64	theme	fatty	1012:1016	arg1	acids					1018:1022	branched-chain fatty acids	997:1022	branched-chain fatty acids (BCFAs)	997:1030	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	8	65	theme	S.	1114:1115	arg1	counts					1129:1134	More S. Typhimurium counts	1109:1134	More S. Typhimurium counts in feces and other tissues	1109:1161	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	8	66	located	observed	1168:1175	arg1	mice					1188:1191	HSD-fed mice	1180:1191	HSD-fed mice	1180:1191	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	8	66	located	observed	1168:1175	arg2	counts					1129:1134	More S. Typhimurium counts	1109:1134	More S. Typhimurium counts in feces and other tissues	1109:1161	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	2	67	from	impact	412:417	arg1	development					447:457	the development	443:457	the development of certain diseases, particularly enteric infections	443:510	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	1	68	theme	westernized	135:145	arg1	diet					147:150	A westernized diet	133:150	A westernized diet characterized by high fat and sugar	133:186	A westernized diet characterized by high fat and sugar is tightly associated with the development of metabolic diseases and inflammatory bowel disease.
36880221	8	69	theme	HSD-fed	1180:1186	arg1	mice					1188:1191	HSD-fed mice	1180:1191	HSD-fed mice	1180:1191	More S. Typhimurium counts in feces and other tissues were observed in HSD-fed mice after infection.
36880221	5	70	theme	certain	826:832	arg1	taxa					844:847	certain microbial taxa	826:847	certain microbial taxa	826:847	The high-sugar diet profoundly altered the relative abundance of certain microbial taxa.
36880221	4	71	theme	high	671:674	arg1	HSD					690:692	HSD	690:692	HSD	690:692	C57BL/6 mice received a normal diet (Control) or a high sucrose diet (HSD) for eight weeks and then were infected by Salmonella Typhimurium.
36880221	4	71	theme	high	671:674	arg1	diet					684:687	a high sucrose diet	669:687	a high sucrose diet (HSD)	669:693	C57BL/6 mice received a normal diet (Control) or a high sucrose diet (HSD) for eight weeks and then were infected by Salmonella Typhimurium.
36880221	9	72	theme	junction	1216:1223	arg1	proteins					1225:1232	Tight junction proteins	1210:1232	Tight junction proteins	1210:1232	Tight junction proteins and antimicrobial peptides were significantly decreased in HSD-fed mice.
36880221	1	73	theme	metabolic	234:242	arg1	diseases					244:251	metabolic diseases	234:251	metabolic diseases	234:251	A westernized diet characterized by high fat and sugar is tightly associated with the development of metabolic diseases and inflammatory bowel disease.
36880221	2	74	theme	enteric	493:499	arg1	infections					501:510	enteric infections	493:510	enteric infections	493:510	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	2	74	theme	enteric	493:499	arg1	diseases					470:477	certain diseases	462:477	certain diseases	462:477	Although a high-fat diet has been extensively studied for its involvement in various diseases, fewer studies have examined the impact of a high-sugar diet on the development of certain diseases, particularly enteric infections.
36880221	6	75	theme	diet-fed	915:922	arg1	mice					924:927	normal diet-fed mice	908:927	normal diet-fed mice	908:927	Bacteroidetes and Verrucomicrobiota were more abundant in normal diet-fed mice than in HSD-fed mice.
36880221	4	76	theme	C57BL/6	620:626	arg1	mice					628:631	C57BL/6 mice	620:631	C57BL/6 mice	620:631	C57BL/6 mice received a normal diet (Control) or a high sucrose diet (HSD) for eight weeks and then were infected by Salmonella Typhimurium.
36880221	7	77	theme	short-chain	961:971	arg1	acids					979:983	short-chain fatty acids	961:983	short-chain fatty acids (SCFAs)	961:991	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	7	77	theme	short-chain	961:971	arg1	SCFAs					986:990	SCFAs	986:990	SCFAs	986:990	Moreover, short-chain fatty acids (SCFAs) and branched-chain fatty acids (BCFAs) were significantly higher in mice from the control group than the HSD group.
36880221	1	78	theme	diseases	244:251	arg1	development					219:229	the development	215:229	the development of metabolic diseases and inflammatory bowel disease	215:282	A westernized diet characterized by high fat and sugar is tightly associated with the development of metabolic diseases and inflammatory bowel disease.
35907356	0	0	theme	ZnO	124:126	arg1	nanoparticles					128:140	carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles	83:140	carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles	83:140	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	5	1	theme	high	1218:1221	arg1	%					1246:1246	~3089%	1241:1246	~3089%	1241:1246	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	1	theme	high	1218:1221	arg1	absorption					1229:1238	high water absorption	1218:1238	high water absorption (~3089%)	1218:1247	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	6	2	theme	antibacterial	1352:1364	arg1	potential					1366:1374	antibacterial potential	1352:1374	antibacterial potential of the composite sponge	1352:1398	The incorporation of Cu-doped ZnO nanoparticles enhanced antibacterial potential of the composite sponge.
35907356	8	3	theme	dressing	1615:1622	arg1	candidate					1639:1647	a promising candidate	1627:1647	a promising candidate for managing infected wounds	1627:1676	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	8	3	theme	dressing	1615:1622	arg1	potential					1557:1565	the potential	1553:1565	the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1553:1622	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	4	4	theme	process	768:774	arg1	effect					747:752	the effect	743:752	the effect of the doping process on the ZnO crystalline structure	743:807	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	8	5	theme	ZnO	1611:1613	arg1	dressing					1615:1622	carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1570:1622	carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1570:1622	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	1	6	theme	composite	335:343	arg1	sponge					345:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge	272:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method	272:394	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	0	7	theme	cellulose/gelatin/Cu-doped	97:122	arg1	nanoparticles					128:140	carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles	83:140	carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles	83:140	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	5	8	theme	water	1223:1227	arg1	%					1246:1246	~3089%	1241:1246	~3089%	1241:1246	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	8	theme	water	1223:1227	arg1	absorption					1229:1238	high water absorption	1218:1238	high water absorption (~3089%)	1218:1247	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	1	9	theme	superabsorbent	197:210	arg1	dressing					212:219	a novel antibacterial and superabsorbent dressing	171:219	a novel antibacterial and superabsorbent dressing	171:219	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	8	10	theme	promising	1629:1637	arg1	candidate					1639:1647	a promising candidate	1627:1647	a promising candidate for managing infected wounds	1627:1676	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	8	10	theme	promising	1629:1637	arg1	potential					1557:1565	the potential	1553:1565	the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1553:1622	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	6	11	theme	nanoparticles	1329:1341	arg1	incorporation					1299:1311	The incorporation	1295:1311	The incorporation of Cu-doped ZnO nanoparticles	1295:1341	The incorporation of Cu-doped ZnO nanoparticles enhanced antibacterial potential of the composite sponge.
35907356	5	12	theme	mechanical	1038:1047	arg1	performance					1049:1059	the physical and mechanical performance	1021:1059	the physical and mechanical performance of the optimized composite sponge	1021:1093	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	3	13	theme	copper	660:665	arg1	concentration					643:655	the concentration	639:655	the concentration of copper in ZnO	639:672	The XRD evaluation determined that the concentration of copper in ZnO is limited to below 5%.
35907356	4	14	dep	aureus	939:944	arg1	strains					977:983	bacterial strains	967:983	bacterial strains	967:983	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	6	15	theme	ZnO	1325:1327	arg1	nanoparticles					1329:1341	Cu-doped ZnO nanoparticles	1316:1341	Cu-doped ZnO nanoparticles	1316:1341	The incorporation of Cu-doped ZnO nanoparticles enhanced antibacterial potential of the composite sponge.
35907356	4	16	theme	zinc	827:830	arg1	ions					843:846	more zinc and copper ions	822:846	more zinc and copper ions	822:846	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	4	17	from	effect	747:752	arg1	structure					799:807	the ZnO crystalline structure	779:807	the ZnO crystalline structure	779:807	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	4	18	theme	ZnO	783:785	arg1	structure					799:807	the ZnO crystalline structure	779:807	the ZnO crystalline structure	779:807	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	8	19	theme	cellulose/gelatin/Cu-doped	1584:1609	arg1	dressing					1615:1622	carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1570:1622	carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1570:1622	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	6	20	theme	Cu-doped	1316:1323	arg1	nanoparticles					1329:1341	Cu-doped ZnO nanoparticles	1316:1341	Cu-doped ZnO nanoparticles	1316:1341	The incorporation of Cu-doped ZnO nanoparticles enhanced antibacterial potential of the composite sponge.
35907356	2	21	theme	agent-used	508:517	arg1	process					533:539	the stabilizing agent-used precipitation process	492:539	the stabilizing agent-used precipitation process	492:539	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	7	22	theme	human	1484:1488	arg1	cells					1508:1512	human dermal fibroblast cells	1484:1512	human dermal fibroblast cells	1484:1512	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	5	23	theme	ZnO	1000:1002	arg1	nanoparticles					1004:1016	ZnO nanoparticles	1000:1016	ZnO nanoparticles	1000:1016	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	0	24	theme	composite	54:62	arg1	sponges					64:70	antibacterial and superabsorbent wound composite sponges	15:70	antibacterial and superabsorbent wound composite sponges	15:70	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	4	25	theme	antibacterial	893:905	arg1	activity					907:914	antibacterial activity	893:914	antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains	893:983	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	4	26	theme	copper	836:841	arg1	ions					843:846	more zinc and copper ions	822:846	more zinc and copper ions	822:846	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	3	27	theme	XRD	608:610	arg1	evaluation					612:621	The XRD evaluation	604:621	The XRD evaluation	604:621	The XRD evaluation determined that the concentration of copper in ZnO is limited to below 5%.
35907356	1	28	link	glutaraldehyde-crosslinked	308:333	arg1	sponge					345:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge	272:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method	272:394	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	2	29	theme	Cu-doped	409:416	arg1	nanoparticles					453:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles	397:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles	397:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	8	30	theme	carboxymethyl	1570:1582	arg1	dressing					1615:1622	carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1570:1622	carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing	1570:1622	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	7	31	theme	fibroblast	1497:1506	arg1	cells					1508:1512	human dermal fibroblast cells	1484:1512	human dermal fibroblast cells	1484:1512	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	5	32	from	effect	990:995	arg1	performance					1049:1059	the physical and mechanical performance	1021:1059	the physical and mechanical performance of the optimized composite sponge	1021:1093	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	7	33	theme	cells	1508:1512	arg1	adhesion					1472:1479	adhesion	1472:1479	adhesion	1472:1479	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	7	33	theme	cells	1508:1512	arg1	proliferation					1454:1466	proliferation	1454:1466	proliferation	1454:1466	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	7	33	theme	cells	1508:1512	arg1	viability					1443:1451	viability	1443:1451	viability	1443:1451	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	5	34	theme	proper	1253:1258	arg1	elongation					1260:1269	proper elongation	1253:1269	proper elongation (~49%)	1253:1276	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	34	theme	proper	1253:1258	arg1	%					1275:1275	~49%	1272:1275	~49%	1272:1275	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	35	theme	nanoparticles	1004:1016	arg1	effect					990:995	The effect	986:995	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge	986:1093	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	2	36	theme	Undoped	397:403	arg1	nanoparticles					453:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles	397:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles	397:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	2	37	theme	precipitation	519:531	arg1	process					533:539	the stabilizing agent-used precipitation process	492:539	the stabilizing agent-used precipitation process	492:539	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	0	38	theme	antibacterial	15:27	arg1	sponges					64:70	antibacterial and superabsorbent wound composite sponges	15:70	antibacterial and superabsorbent wound composite sponges	15:70	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	1	39	theme	lyophilization	374:387	arg1	method					389:394	lyophilization method	374:394	lyophilization method	374:394	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	5	40	theme	physical	1025:1032	arg1	performance					1049:1059	the physical and mechanical performance	1021:1059	the physical and mechanical performance of the optimized composite sponge	1021:1093	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	4	41	theme	doping	761:766	arg1	process					768:774	the doping process	757:774	the doping process	757:774	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	1	42	theme	ZnO	249:251	arg1	nanoparticles					253:265	the Cu-doped ZnO nanoparticles	236:265	the Cu-doped ZnO nanoparticles	236:265	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	0	43	theme	wound	48:52	arg1	sponges					64:70	antibacterial and superabsorbent wound composite sponges	15:70	antibacterial and superabsorbent wound composite sponges	15:70	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	4	44	theme	ICP-OES	716:722	arg1	analysis					724:731	The ICP-OES analysis	712:731	The ICP-OES analysis	712:731	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	2	45	theme	stabilizing	496:506	arg1	process					533:539	the stabilizing agent-used precipitation process	492:539	the stabilizing agent-used precipitation process	492:539	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	0	46	theme	superabsorbent	33:46	arg1	sponges					64:70	antibacterial and superabsorbent wound composite sponges	15:70	antibacterial and superabsorbent wound composite sponges	15:70	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	8	47	theme	infected	1662:1669	arg1	wounds					1671:1676	infected wounds	1662:1676	infected wounds	1662:1676	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	6	48	theme	sponge	1393:1398	arg1	potential					1366:1374	antibacterial potential	1352:1374	antibacterial potential of the composite sponge	1352:1398	The incorporation of Cu-doped ZnO nanoparticles enhanced antibacterial potential of the composite sponge.
35907356	5	49	theme	well-interconnected	1166:1184	arg1	~156 µm					1204:1210	~156 µm	1204:1210	~156 µm	1204:1210	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	49	theme	well-interconnected	1166:1184	arg1	structure					1193:1201	a well-interconnected porous structure	1164:1201	a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%)	1164:1276	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	0	50	theme	sponges	64:70	arg1	Development					0:10	Development	0:10	Development of antibacterial and superabsorbent wound composite sponges	0:70	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	2	51	theme	ICP-OES	584:590	arg1	techniques					592:601	XRD, FESEM, FTIR, and ICP-OES techniques	562:601	techniques	592:601	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	6	52	theme	composite	1383:1391	arg1	sponge					1393:1398	the composite sponge	1379:1398	the composite sponge	1379:1398	The incorporation of Cu-doped ZnO nanoparticles enhanced antibacterial potential of the composite sponge.
35907356	5	53	theme	porous	1186:1191	arg1	~156 µm					1204:1210	~156 µm	1204:1210	~156 µm	1204:1210	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	53	theme	porous	1186:1191	arg1	structure					1193:1201	a well-interconnected porous structure	1164:1201	a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%)	1164:1276	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	2	54	theme	XRD	562:564	arg1	FTIR					574:577	XRD, FESEM, FTIR, and ICP-OES techniques	562:601	FTIR	574:577	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	2	54	theme	XRD	562:564	arg1	FESEM					567:571	FESEM	567:571	FESEM	567:571	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	4	55	theme	bacterial	967:975	arg1	strains					977:983	bacterial strains	967:983	bacterial strains	967:983	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	5	56	theme	3 wt	1131:1134	arg1	nanoparticles					1141:1153	3 wt% ZnO nanoparticles	1131:1153	3 wt% ZnO nanoparticles	1131:1153	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	8	57	theme	obtained	1528:1535	arg1	data					1537:1540	the obtained data	1524:1540	the obtained data	1524:1540	Overall, the obtained data has proved the potential of carboxymethyl cellulose/gelatin/Cu-doped ZnO dressing as a promising candidate for managing infected wounds.
35907356	3	58	from	concentration	643:655	arg1	ZnO					670:672	ZnO	670:672	ZnO	670:672	The XRD evaluation determined that the concentration of copper in ZnO is limited to below 5%.
35907356	5	59	theme	wet	1283:1285	arg1	medium					1287:1292	a wet medium	1281:1292	a wet medium	1281:1292	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	60	theme	%	1135:1135	arg1	nanoparticles					1141:1153	3 wt% ZnO nanoparticles	1131:1153	3 wt% ZnO nanoparticles	1131:1153	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	4	61	theme	Cu-doped	853:860	arg1	ZnO					862:864	Cu-doped ZnO	853:864	Cu-doped ZnO	853:864	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	4	62	theme	crystalline	787:797	arg1	structure					799:807	the ZnO crystalline structure	779:807	the ZnO crystalline structure	779:807	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	4	63	theme	more	822:825	arg1	ions					843:846	more zinc and copper ions	822:846	more zinc and copper ions	822:846	Additionally, The ICP-OES analysis confirmed the effect of the doping process on the ZnO crystalline structure by releasing more zinc and copper ions from Cu-doped ZnO, which resulted to improve antibacterial activity against Staphylococcus aureus and Escherichia coli bacterial strains.
35907356	7	64	theme	dermal	1490:1495	arg1	cells					1508:1512	human dermal fibroblast cells	1484:1512	human dermal fibroblast cells	1484:1512	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	5	65	theme	ZnO	1137:1139	arg1	nanoparticles					1141:1153	3 wt% ZnO nanoparticles	1131:1153	3 wt% ZnO nanoparticles	1131:1153	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	66	theme	composite	1078:1086	arg1	sponge					1088:1093	the optimized composite sponge	1064:1093	the optimized composite sponge	1064:1093	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	2	67	theme	ZnO	418:420	arg1	nanoparticles					453:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles	397:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles	397:465	Undoped and Cu-doped ZnO (Zn1-xCuxO, x = 0.03 and 0.05) nanoparticles were synthesized through the stabilizing agent-used precipitation process and characterized by XRD, FESEM, FTIR, and ICP-OES techniques.
35907356	5	68	with	structure	1193:1201	arg1	%					1246:1246	~3089%	1241:1246	~3089%	1241:1246	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	68	with	structure	1193:1201	arg1	elongation					1260:1269	proper elongation	1253:1269	proper elongation (~49%)	1253:1276	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	68	with	structure	1193:1201	arg1	%					1275:1275	~49%	1272:1275	~49%	1272:1275	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	68	with	structure	1193:1201	arg1	absorption					1229:1238	high water absorption	1218:1238	high water absorption (~3089%)	1218:1247	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	1	69	theme	carboxymethyl	276:288	arg1	sponge					345:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge	272:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method	272:394	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	0	70	theme	carboxymethyl	83:95	arg1	nanoparticles					128:140	carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles	83:140	carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles	83:140	Development of antibacterial and superabsorbent wound composite sponges containing carboxymethyl cellulose/gelatin/Cu-doped ZnO nanoparticles.
35907356	5	71	theme	nanoparticles	1141:1153	arg1	incorporation					1114:1126	the incorporation	1110:1126	the incorporation of 3 wt% ZnO nanoparticles	1110:1153	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	5	72	theme	sponge	1088:1093	arg1	performance					1049:1059	the physical and mechanical performance	1021:1059	the physical and mechanical performance of the optimized composite sponge	1021:1093	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	1	73	theme	Cu-doped	240:247	arg1	nanoparticles					253:265	the Cu-doped ZnO nanoparticles	236:265	the Cu-doped ZnO nanoparticles	236:265	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	1	74	theme	novel	173:177	arg1	dressing					212:219	a novel antibacterial and superabsorbent dressing	171:219	a novel antibacterial and superabsorbent dressing	171:219	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	1	75	theme	cellulose/gelatin	290:306	arg1	sponge					345:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge	272:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method	272:394	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	5	76	theme	optimized	1068:1076	arg1	sponge					1088:1093	the optimized composite sponge	1064:1093	the optimized composite sponge	1064:1093	The effect of ZnO nanoparticles on the physical and mechanical performance of the optimized composite sponge indicated that the incorporation of 3 wt% ZnO nanoparticles produces a well-interconnected porous structure (~156 µm) with high water absorption (~3089%) and proper elongation (~49%) in a wet medium.
35907356	7	77	theme	sponge	1416:1421	arg1	groups					1423:1428	all sponge groups	1412:1428	all sponge groups	1412:1428	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	7	77	theme	sponge	1416:1421	arg1	Meanwhile					1401:1409	Meanwhile	1401:1409	Meanwhile	1401:1409	Meanwhile, all sponge groups are safe for viability, proliferation and adhesion of human dermal fibroblast cells.
35907356	1	78	theme	antibacterial	179:191	arg1	dressing					212:219	a novel antibacterial and superabsorbent dressing	171:219	a novel antibacterial and superabsorbent dressing	171:219	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
35907356	1	79	theme	glutaraldehyde-crosslinked	308:333	arg1	sponge					345:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge	272:350	the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method	272:394	This study aimed to develop a novel antibacterial and superabsorbent dressing by introducing the Cu-doped ZnO nanoparticles into the carboxymethyl cellulose/gelatin glutaraldehyde-crosslinked composite sponge that is fabricated by lyophilization method.
36635576	13	0	theme	mixed	1560:1564	arg1	caused the					1587:1596	the mixed carbon source medium caused the high production and broth viscosity	1556:1632	caused the	1587:1596	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	13	1	from	synthesis	1543:1551	arg1	viscosity					1624:1632	broth viscosity	1618:1632	broth viscosity	1618:1632	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	13	1	from	synthesis	1543:1551	arg1	caused the					1587:1596	the mixed carbon source medium caused the high production and broth viscosity	1556:1632	caused the	1587:1596	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	2	2	theme	WL	141:142	arg1	gum					144:146	The polysaccharide WL gum	122:146	The polysaccharide WL gum	122:146	The polysaccharide WL gum is produced by the marine microorganism Sphingomonas sp.
36635576	3	3	theme	commercial	227:236	arg1	utility					238:244	great commercial utility potential	221:254	great commercial utility potential in many industries	221:273	WG and presents great commercial utility potential in many industries especially in oil industries.
36635576	12	4	theme	elastic	1352:1358	arg1	modulus					1360:1366	elastic modulus	1352:1366	elastic modulus	1352:1366	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	13	5	from	level	1474:1478	arg1	synthesis					1543:1551	WL gum synthesis	1536:1551	WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity	1536:1632	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	13	6	dep	caused the	1587:1596	arg1	production					1603:1612	high production	1598:1612	high production	1598:1612	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	13	7	theme	carbon	1566:1571	arg1	caused the					1587:1596	the mixed carbon source medium caused the high production and broth viscosity	1556:1632	caused the	1587:1596	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	10	8	theme	pretreated	1027:1036	arg1	molasses					1038:1045	unpretreated and pretreated molasses	1010:1045	unpretreated and pretreated molasses affecting WL production	1010:1069	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	3	9	theme	many	259:262	arg1	industries					264:273	many industries	259:273	many industries	259:273	WG and presents great commercial utility potential in many industries especially in oil industries.
36635576	11	10	theme	production	1091:1100	arg1	medium					1102:1107	The cost-efficient production medium	1072:1107	The cost-efficient production medium	1072:1107	The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, K2HPO4 1, MgSO4 0.1, ZnSO4 0.1 and the WL gum production reached 40.25 ± 1.15 g/L.
36635576	3	11	theme	potential	246:254	arg1	utility					238:244	great commercial utility potential	221:254	great commercial utility potential in many industries	221:273	WG and presents great commercial utility potential in many industries especially in oil industries.
36635576	8	12	theme	rheological	733:743	arg1	properties					745:754	rheological properties	733:754	rheological properties	733:754	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	7	13	theme	fermentation	681:692	arg1	medium					694:699	the WL gum fermentation medium	670:699	the WL gum fermentation medium	670:699	One-factor design and RSM statistical analysis were employed to optimize the WL gum fermentation medium.
36635576	13	14	theme	genes	1483:1487	arg1	level					1474:1478	The higher expressional level	1450:1478	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.	1450:1633	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	3	15	theme	oil	289:291	arg1	industries					293:302	oil industries	289:302	oil industries	289:302	WG and presents great commercial utility potential in many industries especially in oil industries.
36635576	12	16	theme	higher	1301:1306	arg1	viscosity					1317:1325	the higher apparent viscosity	1297:1325	the higher apparent viscosity	1297:1325	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	7	17	theme	WL	674:675	arg1	medium					694:699	the WL gum fermentation medium	670:699	the WL gum fermentation medium	670:699	One-factor design and RSM statistical analysis were employed to optimize the WL gum fermentation medium.
36635576	12	18	theme	WL	1263:1264	arg1	WL-molasses					1278:1288	The WL gum product WL-molasses	1259:1288	The WL gum product WL-molasses	1259:1288	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	10	19	theme	unpretreated	1010:1021	arg1	molasses					1038:1045	unpretreated and pretreated molasses	1010:1045	unpretreated and pretreated molasses affecting WL production	1010:1069	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	13	20	theme	higher	1454:1459	arg1	level					1474:1478	The higher expressional level	1450:1478	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.	1450:1633	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	5	21	theme	used	498:501	arg1	sources					510:516	the commonly used carbon sources	485:516	the commonly used carbon sources	485:516	Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources.
36635576	12	22	theme	product	1270:1276	arg1	WL-molasses					1278:1288	The WL gum product WL-molasses	1259:1288	The WL gum product WL-molasses	1259:1288	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	8	23	theme	WL	779:780	arg1	gum					782:784	WL gum	779:784	WL gum	779:784	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	0	24	theme	Sphingomonas	52:63	arg1	sp					65:66	Sphingomonas sp	52:66	Sphingomonas sp	52:66	Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp.
36635576	5	25	theme	efficient	384:392	arg1	system					405:410	an efficient production system	381:410	an efficient production system at a lower cost	381:426	Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources.
36635576	11	26	theme	WL	1215:1216	arg1	production					1222:1231	the WL gum production	1211:1231	the WL gum production	1211:1231	The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, K2HPO4 1, MgSO4 0.1, ZnSO4 0.1 and the WL gum production reached 40.25 ± 1.15 g/L.
36635576	7	27	theme	RSM	619:621	arg1	analysis					635:642	RSM statistical analysis	619:642	RSM statistical analysis	619:642	One-factor design and RSM statistical analysis were employed to optimize the WL gum fermentation medium.
36635576	7	28	theme	One-factor	597:606	arg1	design					608:613	One-factor design	597:613	One-factor design	597:613	One-factor design and RSM statistical analysis were employed to optimize the WL gum fermentation medium.
36635576	14	29	theme	WL	1682:1683	arg1	production					1689:1698	WL gum production	1682:1698	WL gum production	1682:1698	This work provided a cost-efficient method for WL gum production.
36635576	9	30	theme	pretreated	838:847	arg1	molasses					854:861	the pretreated beet molasses	834:861	the pretreated beet molasses	834:861	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	0	31	theme	Cost-Efficient	0:13	arg1	Production					15:24	Cost-Efficient Production	0:24	Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp.	0:67	Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp.
36635576	8	32	dep	properties	745:754	arg1	the					729:731	the	729:731	the	729:731	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	14	33	theme	gum	1685:1687	arg1	production					1689:1698	WL gum production	1682:1698	WL gum production	1682:1698	This work provided a cost-efficient method for WL gum production.
36635576	12	34	theme	molecular	1434:1442	arg1	mass					1444:1447	its highest molecular mass	1422:1447	its highest molecular mass	1422:1447	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	5	35	from	cost	423:426	arg1	system					405:410	an efficient production system	381:410	an efficient production system at a lower cost	381:426	Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources.
36635576	13	36	theme	gum	1539:1541	arg1	synthesis					1543:1551	WL gum synthesis	1536:1551	WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity	1536:1632	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	0	37	theme	Sphingan	33:40	arg1	Gum					45:47	the Sphingan WL Gum	29:47	the Sphingan WL Gum	29:47	Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp.
36635576	9	38	dep	production	910:919	arg1	11.16 g/L					937:945	11.16 g/L	937:945	11.16 g/L	937:945	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	9	38	dep	production	910:919	arg1	12.94 Pa·s					922:931	12.94 Pa·s	922:931	12.94 Pa·s	922:931	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	4	39	theme	fermentation	323:334	arg1	cost					336:339	the high fermentation cost	314:339	the high fermentation cost	314:339	However, the high fermentation cost limits its wide application.
36635576	9	40	theme	broth	883:887	arg1	viscosity					889:897	high broth viscosity	878:897	high broth viscosity	878:897	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	0	41	theme	Gum	45:47	arg1	Production					15:24	Cost-Efficient Production	0:24	Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp.	0:67	Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp.
36635576	14	42	theme	cost-efficient	1656:1669	arg1	method					1671:1676	a cost-efficient method	1654:1676	a cost-efficient method for WL gum production	1654:1698	This work provided a cost-efficient method for WL gum production.
36635576	10	43	theme	Heavy	949:953	arg1	ions					961:964	Heavy metal ions	949:964	Heavy metal ions	949:964	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	10	43	theme	Heavy	949:953	arg1	factors					999:1005	the key factors	991:1005	the key factors	991:1005	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	10	43	theme	Heavy	949:953	arg1	ash					970:972	ash	970:972	ash	970:972	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	2	44	theme	microorganism	174:186	arg1	sp					201:202	the marine microorganism Sphingomonas sp	163:202	the marine microorganism Sphingomonas sp	163:202	The polysaccharide WL gum is produced by the marine microorganism Sphingomonas sp.
36635576	9	45	theme	gum	906:908	arg1	production					910:919	WL gum production	903:919	WL gum production (12.94 Pa·s and 11.16 g/L)	903:946	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	6	46	theme	different	524:532	arg1	molasses					534:541	Four different molasses	519:541	Four different molasses	519:541	Four different molasses were screened and their composition was investigated.
36635576	13	47	theme	source	1573:1578	arg1	caused the					1587:1596	the mixed carbon source medium caused the high production and broth viscosity	1556:1632	caused the	1587:1596	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	12	48	theme	viscous	1332:1338	arg1	modulus					1340:1346	viscous modulus	1332:1346	viscous modulus	1332:1346	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	2	49	theme	polysaccharide	126:139	arg1	gum					144:146	The polysaccharide WL gum	122:146	The polysaccharide WL gum	122:146	The polysaccharide WL gum is produced by the marine microorganism Sphingomonas sp.
36635576	4	50	theme	wide	352:355	arg1	application					357:367	its wide application	348:367	its wide application	348:367	However, the high fermentation cost limits its wide application.
36635576	3	51	theme	great	221:225	arg1	utility					238:244	great commercial utility potential	221:254	great commercial utility potential in many industries	221:273	WG and presents great commercial utility potential in many industries especially in oil industries.
36635576	12	52	theme	apparent	1308:1315	arg1	viscosity					1317:1325	the higher apparent viscosity	1297:1325	the higher apparent viscosity	1297:1325	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	13	53	theme	medium	1580:1585	arg1	caused the					1587:1596	the mixed carbon source medium caused the high production and broth viscosity	1556:1632	caused the	1587:1596	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	5	54	theme	beet	450:453	arg1	molasses					455:462	beet molasses	450:462	beet molasses	450:462	Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources.
36635576	11	55	theme	cost-efficient	1076:1089	arg1	medium					1102:1107	The cost-efficient production medium	1072:1107	The cost-efficient production medium	1072:1107	The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, K2HPO4 1, MgSO4 0.1, ZnSO4 0.1 and the WL gum production reached 40.25 ± 1.15 g/L.
36635576	8	56	theme	molasses	717:724	arg1	effects					706:712	The effects	702:712	The effects of molasses on the rheological properties and gene expression of WL gum	702:784	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	10	57	theme	key	995:997	arg1	ions					961:964	Heavy metal ions	949:964	Heavy metal ions	949:964	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	10	57	theme	key	995:997	arg1	factors					999:1005	the key factors	991:1005	the key factors	991:1005	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	10	57	theme	key	995:997	arg1	ash					970:972	ash	970:972	ash	970:972	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	13	58	theme	broth	1618:1622	arg1	viscosity					1624:1632	broth viscosity	1618:1632	broth viscosity	1618:1632	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	7	59	theme	gum	677:679	arg1	medium					694:699	the WL gum fermentation medium	670:699	the WL gum fermentation medium	670:699	One-factor design and RSM statistical analysis were employed to optimize the WL gum fermentation medium.
36635576	1	60	theme	Carbon	106:111	arg1	Molasses					78:85	Molasses	78:85	Molasses	78:85	WG Using Molasses and Sucrose as the Carbon Sources.
36635576	1	60	theme	Carbon	106:111	arg1	Sources					113:119	the Carbon Sources	102:119	the Carbon Sources	102:119	WG Using Molasses and Sucrose as the Carbon Sources.
36635576	1	60	theme	Carbon	106:111	arg1	Sucrose					91:97	Sucrose	91:97	Sucrose	91:97	WG Using Molasses and Sucrose as the Carbon Sources.
36635576	8	61	theme	gene	760:763	arg1	expression					765:774	gene expression	760:774	gene expression	760:774	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	13	62	theme	expressional	1461:1472	arg1	level					1474:1478	The higher expressional level	1450:1478	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.	1450:1633	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	5	63	theme	carbon	503:508	arg1	sources					510:516	the commonly used carbon sources	485:516	the commonly used carbon sources	485:516	Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources.
36635576	12	64	theme	gum	1266:1268	arg1	WL-molasses					1278:1288	The WL gum product WL-molasses	1259:1288	The WL gum product WL-molasses	1259:1288	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	10	65	from	factors	999:1005	arg1	molasses					1038:1045	unpretreated and pretreated molasses	1010:1045	unpretreated and pretreated molasses affecting WL production	1010:1069	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	10	66	theme	WL	1057:1058	arg1	production					1060:1069	WL production	1057:1069	WL production	1057:1069	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	11	67	theme	gum	1218:1220	arg1	production					1222:1231	the WL gum production	1211:1231	the WL gum production	1211:1231	The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, K2HPO4 1, MgSO4 0.1, ZnSO4 0.1 and the WL gum production reached 40.25 ± 1.15 g/L.
36635576	8	68	from	effects	706:712	arg1	properties					745:754	rheological properties	733:754	rheological properties	733:754	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	8	68	from	effects	706:712	arg1	expression					765:774	gene expression	760:774	gene expression	760:774	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	8	69	theme	gum	782:784	arg1	properties					745:754	rheological properties	733:754	rheological properties	733:754	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	8	69	theme	gum	782:784	arg1	expression					765:774	gene expression	760:774	gene expression	760:774	The effects of molasses on the rheological properties and gene expression of WL gum were also investigated.
36635576	13	70	theme	high	1598:1601	arg1	production					1603:1612	high production	1598:1612	high production	1598:1612	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	7	71	theme	statistical	623:633	arg1	analysis					635:642	RSM statistical analysis	619:642	RSM statistical analysis	619:642	One-factor design and RSM statistical analysis were employed to optimize the WL gum fermentation medium.
36635576	11	72	dep	contained	1109:1117	arg1	g/L					1120:1122	g/L	1120:1122	g/L	1120:1122	The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, K2HPO4 1, MgSO4 0.1, ZnSO4 0.1 and the WL gum production reached 40.25 ± 1.15 g/L.
36635576	9	73	theme	beet	849:852	arg1	molasses					854:861	the pretreated beet molasses	834:861	the pretreated beet molasses	834:861	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	11	74	theme	yeast	1156:1160	arg1	extract					1162:1168	yeast extract 1.23	1156:1173	yeast extract 1.23	1156:1173	The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, K2HPO4 1, MgSO4 0.1, ZnSO4 0.1 and the WL gum production reached 40.25 ± 1.15 g/L.
36635576	13	75	theme	WL	1536:1537	arg1	synthesis					1543:1551	WL gum synthesis	1536:1551	WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity	1536:1632	The higher expressional level of genes such as pgm, ugp, ugd, rmlA, welS, and welG in WL gum synthesis in the mixed carbon source medium caused the high production and broth viscosity.
36635576	0	76	theme	WL	42:43	arg1	Gum					45:47	the Sphingan WL Gum	29:47	the Sphingan WL Gum	29:47	Cost-Efficient Production of the Sphingan WL Gum by Sphingomonas sp.
36635576	3	77	from	utility	238:244	arg1	industries					264:273	many industries	259:273	many industries	259:273	WG and presents great commercial utility potential in many industries especially in oil industries.
36635576	12	78	theme	highest	1426:1432	arg1	mass					1444:1447	its highest molecular mass	1422:1447	its highest molecular mass	1422:1447	The WL gum product WL-molasses showed the higher apparent viscosity, and viscous modulus and elastic modulus than WL-sucrose and WL-mix, which might be related to its highest molecular mass.
36635576	9	79	theme	high	878:881	arg1	viscosity					889:897	high broth viscosity	878:897	high broth viscosity	878:897	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	10	80	theme	metal	955:959	arg1	ions					961:964	Heavy metal ions	949:964	Heavy metal ions	949:964	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	10	80	theme	metal	955:959	arg1	factors					999:1005	the key factors	991:1005	the key factors	991:1005	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	10	80	theme	metal	955:959	arg1	ash					970:972	ash	970:972	ash	970:972	Heavy metal ions and ash were found to be the key factors in unpretreated and pretreated molasses affecting WL production.
36635576	4	81	theme	high	318:321	arg1	cost					336:339	the high fermentation cost	314:339	the high fermentation cost	314:339	However, the high fermentation cost limits its wide application.
36635576	2	82	theme	Sphingomonas	188:199	arg1	sp					201:202	the marine microorganism Sphingomonas sp	163:202	the marine microorganism Sphingomonas sp	163:202	The polysaccharide WL gum is produced by the marine microorganism Sphingomonas sp.
36635576	5	83	theme	lower	417:421	arg1	cost					423:426	a lower cost	415:426	a lower cost	415:426	Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources.
36635576	11	84	theme	sucrose	1126:1132	arg1	61.79					1134:1138	sucrose 61.79	1126:1138	sucrose 61.79	1126:1138	The cost-efficient production medium contained (g/L): sucrose 61.79, molasses 9.95, yeast extract 1.23, K2HPO4 1, MgSO4 0.1, ZnSO4 0.1 and the WL gum production reached 40.25 ± 1.15 g/L.
36635576	9	85	theme	WL	903:904	arg1	production					910:919	WL gum production	903:919	WL gum production (12.94 Pa·s and 11.16 g/L)	903:946	The results showed that the pretreated beet molasses generated both high broth viscosity and WL gum production (12.94 Pa·s and 11.16 g/L).
36635576	2	86	theme	marine	167:172	arg1	sp					201:202	the marine microorganism Sphingomonas sp	163:202	the marine microorganism Sphingomonas sp	163:202	The polysaccharide WL gum is produced by the marine microorganism Sphingomonas sp.
36635576	5	87	theme	production	394:403	arg1	system					405:410	an efficient production system	381:410	an efficient production system at a lower cost	381:426	Therefore, an efficient production system at a lower cost was established using beet molasses to partially replace the commonly used carbon sources.
35695541	9	0	theme	%	1640:1640	arg1	contents					1612:1619	water contents	1606:1619	water contents of 60 wt% and 70 wt%	1606:1640	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	6	1	theme	crosslinking	1145:1156	arg1	monomer					1158:1164	crosslinking monomer	1145:1164	crosslinking monomer	1145:1164	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	11	2	theme	heparin	1876:1882	arg1	profiles					1892:1899	desirable heparin release profiles	1866:1899	desirable heparin release profiles	1866:1899	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	6	3	theme	hydrophilic	1220:1230	arg1	monomer					1232:1238	the hydrophilic monomer	1216:1238	the hydrophilic monomer	1216:1238	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	6	3	theme	hydrophilic	1220:1230	arg1	diacrylate					1119:1128	poly(ethylene glycol) diacrylate	1097:1128	poly(ethylene glycol) diacrylate (PEGDA)	1097:1136	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	14	4	theme	potential	2323:2331	arg1	coatings					2351:2358	potential anti-thrombogenic coatings	2323:2358	potential anti-thrombogenic coatings for ECMO	2323:2367	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	14	4	theme	potential	2323:2331	arg1	hydrogels					2309:2317	heparin-loaded PEG hydrogels	2290:2317	heparin-loaded PEG hydrogels	2290:2317	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	3	5	theme	biocompatible	743:755	arg1	Poly					684:687	Poly	684:687	Poly(ethylene glycol) (PEG)	684:710	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	3	5	theme	biocompatible	743:755	arg1	material					774:781	a biocompatible and anti-fouling material	741:781	a biocompatible and anti-fouling material with wide biomedical application	741:814	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	11	6	theme	ECMO	2003:2006	arg1	membranes					2008:2016	the PMP ECMO membranes	1995:2016	the PMP ECMO membranes	1995:2016	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	1	7	theme	haemostasis	285:295	arg1	maintenance					263:273	the maintenance	259:273	the maintenance of normal haemostasis	259:295	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	9	8	theme	sustained	1658:1666	arg1	release					1676:1682	a sustained heparin release	1656:1682	a sustained heparin release	1656:1682	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	7	9	theme	hydrogel	1464:1471	arg1	flow					1441:1444	the flow	1437:1444	the flow of fluid past the hydrogel	1437:1471	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	7	9	theme	hydrogel	1464:1471	arg1	content					1399:1405	water content	1393:1405	water content	1393:1405	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	7	9	theme	hydrogel	1464:1471	arg1	level					1416:1420	loading level	1408:1420	loading level of heparin	1408:1431	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	1	10	theme	critical	195:202	arg1	tool					220:223	a critical life-sustaining tool	193:223	a critical life-sustaining tool	193:223	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	10	theme	critical	195:202	arg1	oxygenation					173:183	Extracorporeal membrane oxygenation	149:183	Extracorporeal membrane oxygenation (ECMO)	149:190	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	9	11	with	hydrogels	1591:1599	arg1	contents					1612:1619	water contents	1606:1619	water contents of 60 wt% and 70 wt%	1606:1640	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	3	12	theme	anti-fouling	761:772	arg1	Poly					684:687	Poly	684:687	Poly(ethylene glycol) (PEG)	684:710	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	3	12	theme	anti-fouling	761:772	arg1	material					774:781	a biocompatible and anti-fouling material	741:781	a biocompatible and anti-fouling material with wide biomedical application	741:814	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	0	13	theme	membrane	127:134	arg1	oxygenation					136:146	extracorporeal membrane oxygenation	112:146	extracorporeal membrane oxygenation	112:146	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	10	14	theme	heparin	1768:1774	arg1	efficacy					1743:1750	The anticoagulation efficacy	1723:1750	The anticoagulation efficacy of the released heparin	1723:1774	The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood.
35695541	6	15	theme	pre-gel	1270:1276	arg1	solution					1278:1285	the pre-gel solution	1266:1285	the pre-gel solution	1266:1285	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	1	16	theme	shear	416:420	arg1	stresses					422:429	excessive shear stresses	406:429	excessive shear stresses	406:429	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	10	17	theme	activated	1807:1815	arg1	time					1826:1829	the activated clotting time	1803:1829	the activated clotting time of whole blood	1803:1844	The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood.
35695541	9	18	theme	PEGDA/PEGMA	1710:1720	arg1	ratio					1701:1705	the ratio	1697:1705	the ratio of PEGDA/PEGMA	1697:1720	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	3	19	theme	biomedical	793:802	arg1	application					804:814	wide biomedical application	788:814	wide biomedical application	788:814	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	2	20	theme	anticoagulant	600:612	arg1	drug					614:617	an anticoagulant drug	597:617	an anticoagulant drug	597:617	From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit.
35695541	11	21	theme	chemical	1971:1978	arg1	composition					1980:1990	the same chemical composition	1962:1990	the same chemical composition as the PMP ECMO membranes	1962:2016	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	1	22	theme	extracorporeal	438:451	arg1	circuit					453:459	the extracorporeal circuit	434:459	the extracorporeal circuit	434:459	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	7	23	theme	fluid	1449:1453	arg1	hydrogel					1464:1471	fluid past the hydrogel	1449:1471	fluid past the hydrogel	1449:1471	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	5	24	theme	anti-thrombogenic	975:991	arg1	hydrogels					962:970	heparin-loaded PEG-based hydrogels	937:970	heparin-loaded PEG-based hydrogels	937:970	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	5	24	theme	anti-thrombogenic	975:991	arg1	coatings					1001:1008	anti-thrombogenic surface coatings	975:1008	anti-thrombogenic surface coatings for ECMO	975:1017	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	6	25	theme	poly	1097:1100	arg1	PEGDA					1131:1135	PEGDA	1131:1135	PEGDA	1131:1135	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	6	25	theme	poly	1097:1100	arg1	monomer					1232:1238	the hydrophilic monomer	1216:1238	the hydrophilic monomer	1216:1238	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	6	25	theme	poly	1097:1100	arg1	diacrylate					1119:1128	poly(ethylene glycol) diacrylate	1097:1128	poly(ethylene glycol) diacrylate (PEGDA)	1097:1136	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	2	26	theme	drug	525:528	arg1	coatings					538:545	drug eluting coatings	525:545	drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug	525:617	From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit.
35695541	12	27	theme	sustained	2039:2047	arg1	release					2057:2063	sustained heparin release	2039:2063	sustained heparin release	2039:2063	The coatings showed sustained heparin release with a cumulative release of 70-80% after 7 days.
35695541	0	28	theme	-based	53:58	arg1	poly					32:35	heparin-loaded poly	17:35	heparin-loaded poly(ethylene glycol)-based	17:58	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	11	29	theme	same	1966:1969	arg1	composition					1980:1990	the same chemical composition	1962:1990	the same chemical composition as the PMP ECMO membranes	1962:2016	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	1	30	with	contact	358:364	arg1	surfaces					382:389	artificial surfaces	371:389	artificial surfaces	371:389	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	30	with	contact	358:364	arg1	stresses					422:429	excessive shear stresses	406:429	excessive shear stresses	406:429	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	30	with	contact	358:364	arg1	hyperoxia					392:400	hyperoxia	392:400	hyperoxia	392:400	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	8	31	theme	crosslinker	1508:1518	arg1	content					1520:1526	increased crosslinker content	1498:1526	increased crosslinker content	1498:1526	Our results showed that increased crosslinker content and decreased water content led to slower heparin release.
35695541	5	32	theme	heparin-loaded	937:950	arg1	hydrogels					962:970	heparin-loaded PEG-based hydrogels	937:970	heparin-loaded PEG-based hydrogels	937:970	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	5	32	theme	heparin-loaded	937:950	arg1	coatings					1001:1008	anti-thrombogenic surface coatings	975:1008	anti-thrombogenic surface coatings for ECMO	975:1017	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	10	33	theme	whole	1834:1838	arg1	blood					1840:1844	whole blood	1834:1844	whole blood	1834:1844	The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood.
35695541	9	34	theme	water	1606:1610	arg1	contents					1612:1619	water contents	1606:1619	water contents of 60 wt% and 70 wt%	1606:1640	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	0	35	theme	surface	91:97	arg1	coatings					99:106	anti-thrombogenic surface coatings	73:106	anti-thrombogenic surface coatings for extracorporeal membrane oxygenation	73:146	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	1	36	theme	artificial	371:380	arg1	surfaces					382:389	artificial surfaces	371:389	artificial surfaces	371:389	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	11	37	with	films	1951:1955	arg1	composition					1980:1990	the same chemical composition	1962:1990	the same chemical composition as the PMP ECMO membranes	1962:2016	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	1	38	theme	Extracorporeal	149:162	arg1	tool					220:223	a critical life-sustaining tool	193:223	a critical life-sustaining tool	193:223	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	38	theme	Extracorporeal	149:162	arg1	oxygenation					173:183	Extracorporeal membrane oxygenation	149:183	Extracorporeal membrane oxygenation (ECMO)	149:190	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	38	theme	Extracorporeal	149:162	arg1	ECMO					186:189	ECMO	186:189	ECMO	186:189	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	8	39	theme	water	1542:1546	arg1	content					1548:1554	decreased water content	1532:1554	decreased water content	1532:1554	Our results showed that increased crosslinker content and decreased water content led to slower heparin release.
35695541	4	40	theme	Unfractionated	817:830	arg1	heparin					832:838	Unfractionated heparin	817:838	Unfractionated heparin	817:838	Unfractionated heparin is the most commonly used anticoagulant for ECMO.
35695541	4	40	theme	Unfractionated	817:830	arg1	anticoagulant					866:878	the most commonly used anticoagulant	843:878	the most commonly used anticoagulant for ECMO	843:887	Unfractionated heparin is the most commonly used anticoagulant for ECMO.
35695541	13	41	theme	PEG	2158:2160	arg1	coatings					2171:2178	PEG hydrogel coatings	2158:2178	PEG hydrogel coatings	2158:2178	Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time.
35695541	2	42	theme	biomaterials	469:480	arg1	perspective					482:492	a biomaterials perspective	467:492	a biomaterials perspective	467:492	From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit.
35695541	14	43	theme	heparin-loaded	2290:2303	arg1	coatings					2351:2358	potential anti-thrombogenic coatings	2323:2358	potential anti-thrombogenic coatings for ECMO	2323:2367	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	14	43	theme	heparin-loaded	2290:2303	arg1	hydrogels					2309:2317	heparin-loaded PEG hydrogels	2290:2317	heparin-loaded PEG hydrogels	2290:2317	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	13	44	theme	plasma	2234:2239	arg1	time					2257:2260	plasma recalcification time	2234:2260	plasma recalcification time	2234:2260	Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time.
35695541	8	45	theme	slower	1563:1568	arg1	release					1578:1584	slower heparin release	1563:1584	slower heparin release	1563:1584	Our results showed that increased crosslinker content and decreased water content led to slower heparin release.
35695541	0	46	theme	heparin-loaded	17:30	arg1	poly					32:35	heparin-loaded poly	17:35	heparin-loaded poly(ethylene glycol)-based	17:58	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	0	47	dep	-based	53:58	arg1	glycol					46:51	ethylene glycol	37:51	ethylene glycol	37:51	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	4	48	theme	used	861:864	arg1	heparin					832:838	Unfractionated heparin	817:838	Unfractionated heparin	817:838	Unfractionated heparin is the most commonly used anticoagulant for ECMO.
35695541	4	48	theme	used	861:864	arg1	anticoagulant					866:878	the most commonly used anticoagulant	843:878	the most commonly used anticoagulant for ECMO	843:887	Unfractionated heparin is the most commonly used anticoagulant for ECMO.
35695541	13	49	theme	platelet	2202:2209	arg1	adhesion					2211:2218	platelet adhesion	2202:2218	platelet adhesion	2202:2218	Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time.
35695541	7	50	theme	heparin	1330:1336	arg1	release					1319:1325	the release	1315:1325	the release of heparin	1315:1336	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	1	51	theme	circulating	324:334	arg1	blood					336:340	circulating blood	324:340	circulating blood	324:340	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	9	52	theme	heparin	1668:1674	arg1	release					1676:1682	a sustained heparin release	1656:1682	a sustained heparin release	1656:1682	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	6	53	theme	ethylene	1175:1182	arg1	poly					1170:1173	poly	1170:1173	poly(ethylene glycol) methacrylate (PEGMA)	1170:1211	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	6	53	theme	ethylene	1175:1182	arg1	glycol					1184:1189	ethylene glycol	1175:1189	ethylene glycol	1175:1189	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	1	54	theme	significant	232:242	arg1	challenges					244:253	significant challenges	232:253	significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood	232:340	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	6	55	theme	poly	1170:1173	arg1	PEGMA					1206:1210	PEGMA	1206:1210	PEGMA	1206:1210	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	6	55	theme	poly	1170:1173	arg1	methacrylate					1192:1203	poly(ethylene glycol) methacrylate	1170:1203	poly(ethylene glycol) methacrylate (PEGMA)	1170:1211	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	6	56	theme	ethylene	1102:1109	arg1	poly					1097:1100	poly	1097:1100	poly(ethylene glycol) diacrylate (PEGDA)	1097:1136	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	6	56	theme	ethylene	1102:1109	arg1	glycol					1111:1116	ethylene glycol	1102:1116	ethylene glycol	1102:1116	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	10	57	theme	anticoagulation	1727:1741	arg1	efficacy					1743:1750	The anticoagulation efficacy	1723:1750	The anticoagulation efficacy of the released heparin	1723:1774	The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood.
35695541	9	58	theme	wt	1638:1639	arg1	%					1640:1640	60 wt% and 70 wt%	1624:1640	%	1640:1640	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	11	59	theme	desirable	1866:1874	arg1	profiles					1892:1899	desirable heparin release profiles	1866:1899	desirable heparin release profiles	1866:1899	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	11	60	theme	PMP	1999:2001	arg1	membranes					2008:2016	the PMP ECMO membranes	1995:2016	the PMP ECMO membranes	1995:2016	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	1	61	theme	normal	278:283	arg1	haemostasis					285:295	normal haemostasis	278:295	normal haemostasis	278:295	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	7	62	theme	past	1455:1458	arg1	hydrogel					1464:1471	fluid past the hydrogel	1449:1471	fluid past the hydrogel	1449:1471	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	5	63	theme	present	897:903	arg1	study					905:909	the present study	893:909	the present study	893:909	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	2	64	theme	circuit	675:681	arg1	haemocompatibility					649:666	the haemocompatibility	645:666	the haemocompatibility of the circuit	645:681	From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit.
35695541	10	65	theme	released	1759:1766	arg1	heparin					1768:1774	the released heparin	1755:1774	the released heparin	1755:1774	The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood.
35695541	11	66	with	hydrogels	1851:1859	arg1	profiles					1892:1899	desirable heparin release profiles	1866:1899	desirable heparin release profiles	1866:1899	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	3	67	theme	wide	788:791	arg1	application					804:814	wide biomedical application	788:814	wide biomedical application	788:814	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	9	68	theme	wt	1627:1628	arg1	%					1629:1629	60 wt% and 70 wt%	1624:1640	%	1629:1629	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
35695541	1	69	theme	life-sustaining	204:218	arg1	tool					220:223	a critical life-sustaining tool	193:223	a critical life-sustaining tool	193:223	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	69	theme	life-sustaining	204:218	arg1	oxygenation					173:183	Extracorporeal membrane oxygenation	149:183	Extracorporeal membrane oxygenation (ECMO)	149:190	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	7	70	theme	heparin	1425:1431	arg1	flow					1441:1444	the flow	1437:1444	the flow of fluid past the hydrogel	1437:1471	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	7	70	theme	heparin	1425:1431	arg1	content					1399:1405	water content	1393:1405	water content	1393:1405	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	7	70	theme	heparin	1425:1431	arg1	level					1416:1420	loading level	1408:1420	loading level of heparin	1408:1431	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	1	71	theme	excessive	406:414	arg1	stresses					422:429	excessive shear stresses	406:429	excessive shear stresses	406:429	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	10	72	theme	clotting	1817:1824	arg1	time					1826:1829	the activated clotting time	1803:1829	the activated clotting time of whole blood	1803:1844	The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood.
35695541	2	73	theme	haemocompatible	559:573	arg1	hydrogels					575:583	haemocompatible hydrogels	559:583	haemocompatible hydrogels loaded with an anticoagulant drug	559:617	From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit.
35695541	12	74	theme	%	2099:2099	arg1	release					2083:2089	a cumulative release	2070:2089	a cumulative release of 70-80% after 7 days	2070:2112	The coatings showed sustained heparin release with a cumulative release of 70-80% after 7 days.
35695541	13	75	theme	Haemocompatibility	2115:2132	arg1	tests					2134:2138	Haemocompatibility tests	2115:2138	Haemocompatibility tests	2115:2138	Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time.
35695541	5	76	theme	PEG-based	952:960	arg1	hydrogels					962:970	heparin-loaded PEG-based hydrogels	937:970	heparin-loaded PEG-based hydrogels	937:970	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	5	76	theme	PEG-based	952:960	arg1	coatings					1001:1008	anti-thrombogenic surface coatings	975:1008	anti-thrombogenic surface coatings for ECMO	975:1017	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	10	77	theme	blood	1840:1844	arg1	time					1826:1829	the activated clotting time	1803:1829	the activated clotting time of whole blood	1803:1844	The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood.
35695541	8	78	theme	increased	1498:1506	arg1	content					1520:1526	increased crosslinker content	1498:1526	increased crosslinker content	1498:1526	Our results showed that increased crosslinker content and decreased water content led to slower heparin release.
35695541	7	79	theme	water	1393:1397	arg1	content					1399:1405	water content	1393:1405	water content	1393:1405	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	12	80	theme	cumulative	2072:2081	arg1	release					2083:2089	a cumulative release	2070:2089	a cumulative release of 70-80% after 7 days	2070:2112	The coatings showed sustained heparin release with a cumulative release of 70-80% after 7 days.
35695541	1	81	theme	circuit	453:459	arg1	surfaces					382:389	artificial surfaces	371:389	artificial surfaces	371:389	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	81	theme	circuit	453:459	arg1	stresses					422:429	excessive shear stresses	406:429	excessive shear stresses	406:429	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	81	theme	circuit	453:459	arg1	hyperoxia					392:400	hyperoxia	392:400	hyperoxia	392:400	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	82	theme	membrane	164:171	arg1	tool					220:223	a critical life-sustaining tool	193:223	a critical life-sustaining tool	193:223	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	82	theme	membrane	164:171	arg1	oxygenation					173:183	Extracorporeal membrane oxygenation	149:183	Extracorporeal membrane oxygenation (ECMO)	149:190	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	1	82	theme	membrane	164:171	arg1	ECMO					186:189	ECMO	186:189	ECMO	186:189	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	7	83	theme	PEGDA/PEGMA	1380:1390	arg1	ratio					1371:1375	the ratio	1367:1375	the ratio of PEGDA/PEGMA	1367:1390	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	13	84	theme	hydrogel	2162:2169	arg1	coatings					2171:2178	PEG hydrogel coatings	2158:2178	PEG hydrogel coatings	2158:2178	Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time.
35695541	0	85	theme	anti-thrombogenic	73:89	arg1	coatings					99:106	anti-thrombogenic surface coatings	73:106	anti-thrombogenic surface coatings for extracorporeal membrane oxygenation	73:146	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	8	86	theme	decreased	1532:1540	arg1	content					1548:1554	decreased water content	1532:1554	decreased water content	1532:1554	Our results showed that increased crosslinker content and decreased water content led to slower heparin release.
35695541	12	87	theme	heparin	2049:2055	arg1	release					2057:2063	sustained heparin release	2039:2063	sustained heparin release	2039:2063	The coatings showed sustained heparin release with a cumulative release of 70-80% after 7 days.
35695541	14	88	theme	anti-thrombogenic	2333:2349	arg1	coatings					2351:2358	potential anti-thrombogenic coatings	2323:2358	potential anti-thrombogenic coatings for ECMO	2323:2367	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	14	88	theme	anti-thrombogenic	2333:2349	arg1	hydrogels					2309:2317	heparin-loaded PEG hydrogels	2290:2317	heparin-loaded PEG hydrogels	2290:2317	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	0	89	theme	extracorporeal	112:125	arg1	oxygenation					136:146	extracorporeal membrane oxygenation	112:146	extracorporeal membrane oxygenation	112:146	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	2	90	theme	eluting	530:536	arg1	coatings					538:545	drug eluting coatings	525:545	drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug	525:617	From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit.
35695541	14	91	theme	PEG	2305:2307	arg1	coatings					2351:2358	potential anti-thrombogenic coatings	2323:2358	potential anti-thrombogenic coatings for ECMO	2323:2367	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	14	91	theme	PEG	2305:2307	arg1	hydrogels					2309:2317	heparin-loaded PEG hydrogels	2290:2317	heparin-loaded PEG hydrogels	2290:2317	These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
35695541	11	92	theme	poly	1920:1923	arg1	films					1951:1955	poly(4-methyl-1-pentene) (PMP) films	1920:1955	poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes	1920:2016	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	3	93	with	material	774:781	arg1	application					804:814	wide biomedical application	788:814	wide biomedical application	788:814	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	5	94	theme	surface	993:999	arg1	hydrogels					962:970	heparin-loaded PEG-based hydrogels	937:970	heparin-loaded PEG-based hydrogels	937:970	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	5	94	theme	surface	993:999	arg1	coatings					1001:1008	anti-thrombogenic surface coatings	975:1008	anti-thrombogenic surface coatings for ECMO	975:1017	In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated.
35695541	8	95	theme	heparin	1570:1576	arg1	release					1578:1584	slower heparin release	1563:1584	slower heparin release	1563:1584	Our results showed that increased crosslinker content and decreased water content led to slower heparin release.
35695541	0	96	theme	poly	32:35	arg1	Investigation					0:12	Investigation	0:12	Investigation of heparin-loaded poly(ethylene glycol)-based	0:58	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	13	97	theme	recalcification	2241:2255	arg1	time					2257:2260	plasma recalcification time	2234:2260	plasma recalcification time	2234:2260	Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time.
35695541	7	98	theme	loading	1408:1414	arg1	level					1416:1420	loading level	1408:1420	loading level of heparin	1408:1431	Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel.
35695541	3	99	theme	ethylene	689:696	arg1	Poly					684:687	Poly	684:687	Poly(ethylene glycol) (PEG)	684:710	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	3	99	theme	ethylene	689:696	arg1	glycol					698:703	ethylene glycol	689:703	ethylene glycol	689:703	Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application.
35695541	11	100	theme	release	1884:1890	arg1	profiles					1892:1899	desirable heparin release profiles	1866:1899	desirable heparin release profiles	1866:1899	The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes.
35695541	0	101	theme	ethylene	37:44	arg1	glycol					46:51	ethylene glycol	37:51	ethylene glycol	37:51	Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.
35695541	1	102	theme	large	308:312	arg1	volume					314:319	the large volume	304:319	the large volume of circulating blood	304:340	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	6	103	dep	monomer	1158:1164	arg1	the					1141:1143	the	1141:1143	the	1141:1143	The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution.
35695541	1	104	theme	blood	336:340	arg1	volume					314:319	the large volume	304:319	the large volume of circulating blood	304:340	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit.
35695541	9	105	theme	%	1629:1629	arg1	contents					1612:1619	water contents	1606:1619	water contents of 60 wt% and 70 wt%	1606:1640	The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA.
36320702	0	0	theme	protein	85:91	arg1	RBD					93:95	Spike protein RBD	79:95	Spike protein RBD	79:95	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	1	1	theme	neutralizing	332:343	arg1	antibodies					345:354	neutralizing antibodies	332:354	neutralizing antibodies	332:354	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	4	2	theme	mass	941:944	arg1	MS					960:961	MS	960:961	MS	960:961	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	4	2	theme	mass	941:944	arg1	spectrometry					946:957	high-resolution top-down mass spectrometry	916:957	high-resolution top-down mass spectrometry (MS)	916:962	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	3	3	theme	principal	509:517	arg1	target					519:524	the principal target	505:524	the principal target for neutralizing antibodies and therapeutics	505:569	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	1	4	theme	S	239:239	arg1	S-RBD					266:270	S-RBD	266:270	S-RBD	266:270	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	1	4	theme	S	239:239	arg1	domain					258:263	the S receptor-binding domain	235:263	the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies	235:354	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	0	5	theme	Spike	79:83	arg1	RBD					93:95	Spike protein RBD	79:95	Spike protein RBD	79:95	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	1	6	theme	antibodies	345:354	arg1	transmissibility					300:315	transmissibility	300:315	transmissibility	300:315	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	1	6	theme	antibodies	345:354	arg1	evasion					321:327	evasion	321:327	evasion	321:327	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	1	7	theme	B.1.1.529	161:169	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	1	8	theme	receptor-binding	241:256	arg1	S-RBD					266:270	S-RBD	266:270	S-RBD	266:270	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	1	8	theme	receptor-binding	241:256	arg1	domain					258:263	the S receptor-binding domain	235:263	the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies	235:354	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	7	9	theme	O-glycoforms	1255:1266	arg1	utilization					1278:1288	their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1272:1450	their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1272:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	7	9	theme	O-glycoforms	1255:1266	arg1	detail					1237:1242	high resolution detail	1221:1242	high resolution detail of Omicron O-glycoforms	1221:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	2	10	from	protection	450:459	arg1	escape					424:429	viral escape	418:429	viral escape from immunological protection	418:459	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.
36320702	5	11	theme	Omicron	1008:1014	arg1	variant					1016:1022	the Omicron variant	1004:1022	the Omicron variant	1004:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.
36320702	7	12	theme	Omicron	1360:1366	arg1	variant					1368:1374	the Omicron variant	1356:1374	the Omicron variant which could shed light on how this variant escapes immunological protection	1356:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	6	13	dep	Core	1081:1084	arg1	structures					1108:1117	O-glycan structures	1099:1117	O-glycan structures	1099:1117	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.
36320702	6	13	dep	Core	1081:1084	arg1	the					1077:1079	the	1077:1079	the	1077:1079	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.
36320702	3	14	theme	vaccines	723:730	arg1	design					713:718	rational design	704:718	rational design of vaccines and therapeutics	704:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	7	15	theme	direct	1301:1306	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	0	16	theme	top-down	109:116	arg1	spectrometry					123:134	top-down mass spectrometry	109:134	top-down mass spectrometry	109:134	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	7	17	theme	molecular	1308:1316	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	3	18	dep	remains	497:503	arg1	inform					697:702	inform	697:702	can inform rational design of vaccines and therapeutics	693:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	2	19	theme	Omicron	394:400	arg1	variant					402:408	the Omicron variant	390:408	the Omicron variant	390:408	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.
36320702	5	20	gly	O-glycosite	973:983	arg2	Thr376					986:991	Thr376	986:991	Thr376	986:991	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.
36320702	5	20	gly	O-glycosite	973:983	arg2	O-glycosite					973:983	A novel O-glycosite	965:983	A novel O-glycosite (Thr376) unique to the Omicron variant	965:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.
36320702	5	21	theme	unique	994:999	arg1	O-glycosite					973:983	A novel O-glycosite	965:983	A novel O-glycosite (Thr376) unique to the Omicron variant	965:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.
36320702	5	21	theme	unique	994:999	arg1	Thr376					986:991	Thr376	986:991	Thr376	986:991	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.
36320702	4	22	theme	S-RBD	829:833	arg1	variant					835:841	the Omicron S-RBD variant	817:841	the Omicron S-RBD variant	817:841	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	0	23	theme	core	9:12	arg1	glycan					14:19	Distinct core glycan	0:19	Distinct core glycan	0:19	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	7	24	theme	Omicron	1247:1253	arg1	O-glycoforms					1255:1266	Omicron O-glycoforms	1247:1266	Omicron O-glycoforms	1247:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	3	25	theme	structural	581:590	arg1	insights					592:599	new structural insights	577:599	new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes	577:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	0	26	theme	Distinct	0:7	arg1	glycan					14:19	Distinct core glycan	0:19	Distinct core glycan	0:19	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	4	27	theme	molecular	769:777	arg1	variations					779:788	molecular variations	769:788	molecular variations	769:788	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	1	28	theme	numerous	190:197	arg1	mutations					209:217	numerous spike (S) mutations	190:217	numerous spike (S) mutations	190:217	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	2	29	theme	immunological	436:448	arg1	protection					450:459	immunological protection	436:459	immunological protection	436:459	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.
36320702	6	30	theme	structural	1153:1162	arg1	heterogeneity					1164:1176	the O-glycoform structural heterogeneity	1137:1176	the O-glycoform structural heterogeneity of the three variants	1137:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.
36320702	4	31	theme	O-glycoform	794:804	arg1	changes					806:812	O-glycoform changes	794:812	O-glycoform changes	794:812	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	1	32	theme	spike	199:203	arg1	mutations					209:217	numerous spike (S) mutations	190:217	numerous spike (S) mutations	190:217	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	5	33	theme	novel	967:971	arg1	O-glycosite					973:983	A novel O-glycosite	965:983	A novel O-glycosite (Thr376) unique to the Omicron variant	965:1022	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.
36320702	5	33	theme	novel	967:971	arg1	Thr376					986:991	Thr376	986:991	Thr376	986:991	A novel O-glycosite (Thr376) unique to the Omicron variant is identified.
36320702	0	34	theme	mass	118:121	arg1	spectrometry					123:134	top-down mass spectrometry	109:134	top-down mass spectrometry	109:134	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	3	35	theme	changes	685:691	arg1	characterization					628:643	characterization	628:643	characterization	628:643	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	3	35	theme	changes	685:691	arg1	S-RBD					618:622	Omicron S-RBD	610:622	Omicron S-RBD	610:622	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	3	36	theme	new	577:579	arg1	insights					592:599	new structural insights	577:599	new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes	577:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	2	37	theme	viral	418:422	arg1	escape					424:429	viral escape	418:429	viral escape from immunological protection	418:459	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.
36320702	1	38	contain	possesses	180:188	arg2	mutations					209:217	numerous spike (S) mutations	190:217	numerous spike (S) mutations	190:217	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	1	38	contain	possesses	180:188	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	3	39	theme	Omicron	610:616	arg1	S-RBD					618:622	Omicron S-RBD	610:622	Omicron S-RBD	610:622	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	3	40	dep	S-RBD	618:622	arg1	the					606:608	the	606:608	the	606:608	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	4	41	dep	wild-type	858:866	arg1	variants					901:908	variants	901:908	variants	901:908	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	0	42	theme	O-glycoform	25:35	arg1	utilization					37:47	O-glycoform utilization	25:47	O-glycoform utilization of SARS-CoV-2 Omicron variant	25:77	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	3	43	theme	post-translational	652:669	arg1	changes					685:691	the post-translational glycosylation changes	648:691	the post-translational glycosylation changes	648:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	7	44	theme	proteoform	1330:1339	arg1	alterations					1341:1351	proteoform alterations	1330:1351	proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1330:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	0	45	theme	SARS-CoV-2	52:61	arg1	variant					71:77	SARS-CoV-2 Omicron variant	52:77	SARS-CoV-2 Omicron variant	52:77	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	7	46	theme	high	1221:1224	arg1	detail					1237:1242	high resolution detail	1221:1242	high resolution detail of Omicron O-glycoforms	1221:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	4	47	theme	top-down	932:939	arg1	MS					960:961	MS	960:961	MS	960:961	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	4	47	theme	top-down	932:939	arg1	spectrometry					946:957	high-resolution top-down mass spectrometry	916:957	high-resolution top-down mass spectrometry (MS)	916:962	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	3	48	theme	glycosylation	671:683	arg1	changes					685:691	the post-translational glycosylation changes	648:691	the post-translational glycosylation changes	648:691	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	2	49	from	mutations	377:385	arg1	variant					402:408	the Omicron variant	390:408	the Omicron variant	390:408	But exactly how the mutations in the Omicron variant enhance viral escape from immunological protection remains to be understood.
36320702	6	50	theme	O-glycoform	1141:1151	arg1	heterogeneity					1164:1176	the O-glycoform structural heterogeneity	1137:1176	the O-glycoform structural heterogeneity of the three variants	1137:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.
36320702	4	51	theme	high-resolution	916:930	arg1	MS					960:961	MS	960:961	MS	960:961	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	4	51	theme	high-resolution	916:930	arg1	spectrometry					946:957	high-resolution top-down mass spectrometry	916:957	high-resolution top-down mass spectrometry (MS)	916:962	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	7	52	gly	O-glycoforms	1255:1266	arg1	Omicron					1247:1253	Omicron O-glycoforms	1247:1266	Omicron O-glycoforms	1247:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	7	53	from	alterations	1341:1351	arg1	variant					1368:1374	the Omicron variant	1356:1374	the Omicron variant which could shed light on how this variant escapes immunological protection	1356:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	7	54	from	evidence	1318:1325	arg1	variant					1368:1374	the Omicron variant	1356:1374	the Omicron variant which could shed light on how this variant escapes immunological protection	1356:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	4	55	theme	variant	835:841	arg1	variations					779:788	molecular variations	769:788	molecular variations	769:788	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	4	55	theme	variant	835:841	arg1	changes					806:812	O-glycoform changes	794:812	O-glycoform changes	794:812	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	4	56	dep	variations	779:788	arg1	the					765:767	the	765:767	the	765:767	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	7	57	theme	immunological	1427:1439	arg1	protection					1441:1450	immunological protection	1427:1450	immunological protection	1427:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	4	58	theme	Omicron	821:827	arg1	variant					835:841	the Omicron S-RBD variant	817:841	the Omicron S-RBD variant	817:841	Here we report the molecular variations and O-glycoform changes of the Omicron S-RBD variant as compared to wild-type (WA1/2020) and Delta (B.1.617.2) variants using high-resolution top-down mass spectrometry (MS).
36320702	0	59	dep	glycan	14:19	arg1	RBD					93:95	Spike protein RBD	79:95	Spike protein RBD	79:95	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	6	60	theme	O-glycan	1099:1106	arg1	structures					1108:1117	O-glycan structures	1099:1117	O-glycan structures	1099:1117	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.
36320702	0	61	theme	variant	71:77	arg1	glycan					14:19	Distinct core glycan	0:19	Distinct core glycan	0:19	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	0	61	theme	variant	71:77	arg1	utilization					37:47	O-glycoform utilization	25:47	O-glycoform utilization of SARS-CoV-2 Omicron variant	25:77	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	3	62	theme	rational	704:711	arg1	design					713:718	rational design	704:718	rational design of vaccines and therapeutics	704:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	1	63	dep	spike	199:203	arg1	S					206:206	S	206:206	S	206:206	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	7	64	theme	alterations	1341:1351	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	1	65	theme	SARS-CoV-2	141:150	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	6	66	theme	variants	1191:1198	arg1	heterogeneity					1164:1176	the O-glycoform structural heterogeneity	1137:1176	the O-glycoform structural heterogeneity of the three variants	1137:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.
36320702	0	67	theme	Omicron	63:69	arg1	variant					71:77	SARS-CoV-2 Omicron variant	52:77	SARS-CoV-2 Omicron variant	52:77	Distinct core glycan and O-glycoform utilization of SARS-CoV-2 Omicron variant Spike protein RBD revealed by top-down mass spectrometry.
36320702	3	68	theme	therapeutics	736:747	arg1	design					713:718	rational design	704:718	rational design of vaccines and therapeutics	704:747	The S-RBD remains the principal target for neutralizing antibodies and therapeutics, thus new structural insights into the Omicron S-RBD and characterization of the post-translational glycosylation changes can inform rational design of vaccines and therapeutics.
36320702	6	69	gly	heterogeneity	1164:1176	arg1	variants					1191:1198	the three variants	1181:1198	the three variants	1181:1198	Moreover, we have directly quantified the Core 1 and Core 2 O-glycan structures and characterized the O-glycoform structural heterogeneity of the three variants.
36320702	7	70	from	variant	1368:1374	arg1	evidence					1318:1325	direct molecular evidence	1301:1325	direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection	1301:1450	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36320702	1	71	theme	Omicron	152:158	arg1	variant					172:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant	137:178	The SARS-CoV-2 Omicron (B.1.1.529) variant possesses numerous spike (S) mutations particularly in the S receptor-binding domain (S-RBD) that significantly improve transmissibility and evasion of neutralizing antibodies.
36320702	7	72	theme	resolution	1226:1235	arg1	detail					1237:1242	high resolution detail	1221:1242	high resolution detail of Omicron O-glycoforms	1221:1266	Our findings reveal high resolution detail of Omicron O-glycoforms and their utilization to provide direct molecular evidence of proteoform alterations in the Omicron variant which could shed light on how this variant escapes immunological protection.
36881787	11	0	theme	SNCE	1718:1721	arg1	supplementation					1699:1713	the dietary supplementation	1687:1713	the dietary supplementation of SNCE	1687:1721	Broiler cecal microbiota was impacted by age (P < 0.01) but not by the dietary supplementation of SNCE.
36881787	4	1	theme	body	803:806	arg1	weight					808:813	body weight	803:813	body weight	803:813	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	13	2	theme	SNCE	1884:1887	arg1	characterization					1864:1879	The characterization	1860:1879	The characterization of SNCE	1860:1887	The characterization of SNCE allowed to identify compounds such as eriocitrin, naringin, hesperidin, and POS.
36881787	6	3	from	d	1092:1092	arg1	analysis					1080:1087	microbiota analysis	1069:1087	microbiota analysis at d 7 and 42	1069:1101	One broiler chicken was randomly selected from each pen (10 chickens/group) and ceca samples were collected for microbiota analysis at d 7 and 42.
36881787	1	4	theme	natural	245:251	arg1	SNCE					269:272	SNCE	269:272	SNCE	269:272	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	1	4	theme	natural	245:251	arg1	extract					260:266	a standardized natural citrus extract	230:266	a standardized natural citrus extract (SNCE)	230:273	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	0	5	theme	positive	77:84	arg1	effect					86:91	the positive effect	73:91	the positive effect of standardized natural citrus extract on broiler chickens	73:150	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	1	6	theme	intestinal	325:334	arg1	microbiota					336:345	intestinal microbiota	325:345	intestinal microbiota	325:345	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	6	7	from	42	1100:1101	arg1	analysis					1080:1087	microbiota analysis	1069:1087	microbiota analysis at d 7 and 42	1069:1101	One broiler chicken was randomly selected from each pen (10 chickens/group) and ceca samples were collected for microbiota analysis at d 7 and 42.
36881787	1	8	theme	citrus	253:258	arg1	SNCE					269:272	SNCE	269:272	SNCE	269:272	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	1	8	theme	citrus	253:258	arg1	extract					260:266	a standardized natural citrus extract	230:266	a standardized natural citrus extract (SNCE)	230:273	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	0	9	theme	standardized	96:107	arg1	extract					124:130	standardized natural citrus extract	96:130	standardized natural citrus extract	96:130	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	2	10	theme	broiler	544:550	arg1	chickens					552:559	broiler chickens	544:559	broiler chickens	544:559	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	8	11	theme	pectic	1268:1273	arg1	component					1309:1317	a major component	1301:1317	a major component of the SNCE	1301:1329	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	8	11	theme	pectic	1268:1273	arg1	POS					1293:1295	POS	1293:1295	POS	1293:1295	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	8	11	theme	pectic	1268:1273	arg1	oligosaccharides					1275:1290	pectic oligosaccharides	1268:1290	pectic oligosaccharides (POS)	1268:1296	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	1	12	theme	extract	260:266	arg1	composition					215:225	composition	215:225	composition	215:225	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	1	12	theme	extract	260:266	arg1	effect					204:209	the effect	200:209	the effect	200:209	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	14	13	theme	better	2003:2008	arg1	understanding					2010:2022	a better understanding	2001:2022	a better understanding of the observed effect on broiler chickens' growth performances	2001:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	10	14	theme	P	1609:1609	arg1	diets					1602:1606	the CTL diets	1594:1606	the CTL diets (P < 0.01)	1594:1617	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	10	14	theme	P	1609:1609	arg1	<					1611:1611	P < 0.01	1609:1616	P < 0.01	1609:1616	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	14	15	theme	growth	2068:2073	arg1	performances					2075:2086	broiler chickens' growth performances	2050:2086	broiler chickens' growth performances	2050:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	0	16	theme	citrus	117:122	arg1	extract					124:130	standardized natural citrus extract	96:130	standardized natural citrus extract	96:130	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	2	17	theme	same	579:582	arg1	diet					593:596	the same standard diet	575:596	the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively	575:658	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	2	18	theme	standard	584:591	arg1	diet					593:596	the same standard diet	575:596	the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively	575:658	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	5	19	theme	Litter	881:886	arg1	quality					888:894	Litter quality	881:894	Litter quality	881:894	Litter quality was also weekly recorded while mortality was daily recorded.
36881787	10	20	theme	broiler	1518:1524	arg1	chickens					1526:1533	broiler chickens	1518:1533	broiler chickens fed diets supplemented with SNCE	1518:1566	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	10	21	theme	chickens	1526:1533	arg1	higher					1572:1577	higher	1572:1577	higher	1572:1577	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	10	21	theme	chickens	1526:1533	arg1	weight					1508:1513	the final body weight	1493:1513	the final body weight of broiler chickens fed diets supplemented with SNCE	1493:1566	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	0	22	theme	natural	109:115	arg1	extract					124:130	standardized natural citrus extract	96:130	standardized natural citrus extract	96:130	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	14	23	theme	new	1984:1986	arg1	horizons					1988:1995	new horizons	1984:1995	new horizons	1984:1995	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	12	24	theme	chickens	1850:1857	arg1	microbiota					1828:1837	the cecal microbiota	1818:1837	the cecal microbiota of broiler chickens	1818:1857	Results indicate that SNCE allowed enhancing chickens' performances without any modulation of the cecal microbiota of broiler chickens.
36881787	8	25	theme	SNCE	1243:1246	arg1	characterization					1223:1238	the characterization	1219:1238	the characterization of SNCE	1219:1246	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	11	26	theme	Broiler	1620:1626	arg1	microbiota					1634:1643	Broiler cecal microbiota	1620:1643	Broiler cecal microbiota	1620:1643	Broiler cecal microbiota was impacted by age (P < 0.01) but not by the dietary supplementation of SNCE.
36881787	1	27	theme	study	175:179	arg1	objective					157:165	The objective	153:165	The objective of this study	153:179	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	3	28	theme	dietary	666:672	arg1	treatment					674:682	Each dietary treatment	661:682	Each dietary treatment	661:682	Each dietary treatment was composed of 10 experimental units (pen) of 31 broiler chickens each.
36881787	7	29	theme	Chromatographic	1104:1118	arg1	methods					1120:1126	Chromatographic methods	1104:1126	Chromatographic methods	1104:1126	Chromatographic methods were used to determine molecules that enter into the composition of the SNCE.
36881787	12	30	theme	broiler	1842:1848	arg1	chickens					1850:1857	broiler chickens	1842:1857	broiler chickens	1842:1857	Results indicate that SNCE allowed enhancing chickens' performances without any modulation of the cecal microbiota of broiler chickens.
36881787	10	31	theme	broiler	1464:1470	arg1	chickens					1472:1479	broiler chickens	1464:1479	broiler chickens	1464:1479	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	2	32	theme	one-day-old	363:373	arg1	males					375:379	930 one-day-old males	359:379	930 one-day-old males	359:379	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	0	33	theme	Fine	0:3	arg1	characterization					5:20	Fine characterization	0:20	Fine characterization	0:20	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	4	34	theme	feed	785:788	arg1	consumption					790:800	feed consumption	785:800	feed consumption	785:800	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	0	35	theme	microbiota	26:35	arg1	assessment					37:46	microbiota assessment	26:46	microbiota assessment	26:46	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	3	36	theme	experimental	703:714	arg1	pen					723:725	pen	723:725	pen	723:725	Each dietary treatment was composed of 10 experimental units (pen) of 31 broiler chickens each.
36881787	3	36	theme	experimental	703:714	arg1	units					716:720	10 experimental units	700:720	10 experimental units (pen) of 31 broiler chickens each	700:754	Each dietary treatment was composed of 10 experimental units (pen) of 31 broiler chickens each.
36881787	14	37	from	understanding	2010:2022	arg1	performances					2075:2086	broiler chickens' growth performances	2050:2086	broiler chickens' growth performances	2050:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	14	38	theme	observed	2031:2038	arg1	effect					2040:2045	the observed effect	2027:2045	the observed effect on broiler chickens' growth performances	2027:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	6	39	theme	broiler	961:967	arg1	chicken					969:975	One broiler chicken	957:975	One broiler chicken	957:975	One broiler chicken was randomly selected from each pen (10 chickens/group) and ceca samples were collected for microbiota analysis at d 7 and 42.
36881787	2	40	theme	dietary	409:415	arg1	treatments					417:426	3 dietary treatments	407:426	3 dietary treatments	407:426	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	11	41	theme	cecal	1628:1632	arg1	microbiota					1634:1643	Broiler cecal microbiota	1620:1643	Broiler cecal microbiota	1620:1643	Broiler cecal microbiota was impacted by age (P < 0.01) but not by the dietary supplementation of SNCE.
36881787	0	42	theme	extract	124:130	arg1	effect					86:91	the positive effect	73:91	the positive effect of standardized natural citrus extract on broiler chickens	73:150	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	0	43	from	effect	86:91	arg1	chickens					143:150	broiler chickens	135:150	broiler chickens	135:150	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	6	44	theme	ceca	1037:1040	arg1	samples					1042:1048	ceca samples	1037:1048	ceca samples	1037:1048	One broiler chicken was randomly selected from each pen (10 chickens/group) and ceca samples were collected for microbiota analysis at d 7 and 42.
36881787	14	45	theme	broiler	2050:2056	arg1	performances					2075:2086	broiler chickens' growth performances	2050:2086	broiler chickens' growth performances	2050:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	7	46	theme	SNCE	1200:1203	arg1	composition					1181:1191	the composition	1177:1191	the composition of the SNCE	1177:1203	Chromatographic methods were used to determine molecules that enter into the composition of the SNCE.
36881787	10	47	theme	body	1503:1506	arg1	higher					1572:1577	higher	1572:1577	higher	1572:1577	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	10	47	theme	body	1503:1506	arg1	weight					1508:1513	the final body weight	1493:1513	the final body weight of broiler chickens fed diets supplemented with SNCE	1493:1566	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	2	48	theme	standard	497:504	arg1	diet					506:509	a standard diet	495:509	a standard diet	495:509	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	8	49	from	characterization	1223:1238	arg1	Results					1206:1212	Results	1206:1212	Results from the characterization of SNCE	1206:1246	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	10	50	theme	final	1497:1501	arg1	higher					1572:1577	higher	1572:1577	higher	1572:1577	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	10	50	theme	final	1497:1501	arg1	weight					1508:1513	the final body weight	1493:1513	the final body weight of broiler chickens fed diets supplemented with SNCE	1493:1566	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	1	51	theme	broiler	283:289	arg1	performances					308:319	broiler chickens' growth performances	283:319	broiler chickens' growth performances	283:319	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	0	52	theme	broiler	135:141	arg1	chickens					143:150	broiler chickens	135:150	broiler chickens	135:150	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	0	53	dep	keys	51:54	arg1	understanding					59:71	understanding	59:71	to understanding the positive effect of standardized natural citrus extract on broiler chickens	56:150	Fine characterization and microbiota assessment as keys to understanding the positive effect of standardized natural citrus extract on broiler chickens.
36881787	9	54	theme	secondary	1348:1356	arg1	hesperidin					1393:1402	hesperidin	1393:1402	hesperidin	1393:1402	In addition, 35 secondary metabolites, including eriocitrin, hesperidin, and naringin, were identified.
36881787	9	54	theme	secondary	1348:1356	arg1	metabolites					1358:1368	35 secondary metabolites	1345:1368	35 secondary metabolites	1345:1368	In addition, 35 secondary metabolites, including eriocitrin, hesperidin, and naringin, were identified.
36881787	9	54	theme	secondary	1348:1356	arg1	eriocitrin					1381:1390	eriocitrin	1381:1390	eriocitrin	1381:1390	In addition, 35 secondary metabolites, including eriocitrin, hesperidin, and naringin, were identified.
36881787	9	54	theme	secondary	1348:1356	arg1	naringin					1409:1416	naringin	1409:1416	naringin	1409:1416	In addition, 35 secondary metabolites, including eriocitrin, hesperidin, and naringin, were identified.
36881787	11	55	theme	P	1666:1666	arg1	age					1661:1663	age	1661:1663	age (P < 0.01)	1661:1674	Broiler cecal microbiota was impacted by age (P < 0.01) but not by the dietary supplementation of SNCE.
36881787	11	55	theme	P	1666:1666	arg1	<					1668:1668	P < 0.01	1666:1673	P < 0.01	1666:1673	Broiler cecal microbiota was impacted by age (P < 0.01) but not by the dietary supplementation of SNCE.
36881787	4	56	theme	feed	820:823	arg1	FCR					843:845	FCR	843:845	FCR	843:845	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	4	56	theme	feed	820:823	arg1	ratio					836:840	feed conversion ratio	820:840	feed conversion ratio (FCR)	820:846	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	3	57	dep	chickens	742:749	arg1	each					751:754	each	751:754	each	751:754	Each dietary treatment was composed of 10 experimental units (pen) of 31 broiler chickens each.
36881787	2	58	theme	control	431:437	arg1	treatment					439:447	a control treatment	429:447	a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively	429:658	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	2	58	theme	control	431:437	arg1	CTL					450:452	CTL	450:452	CTL	450:452	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	12	59	theme	microbiota	1828:1837	arg1	modulation					1804:1813	any modulation	1800:1813	any modulation of the cecal microbiota of broiler chickens	1800:1857	Results indicate that SNCE allowed enhancing chickens' performances without any modulation of the cecal microbiota of broiler chickens.
36881787	4	60	theme	Growth	757:762	arg1	ratio					836:840	feed conversion ratio	820:840	feed conversion ratio (FCR)	820:846	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	4	60	theme	Growth	757:762	arg1	consumption					790:800	feed consumption	785:800	feed consumption	785:800	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	4	60	theme	Growth	757:762	arg1	weight					808:813	body weight	803:813	body weight	803:813	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	4	60	theme	Growth	757:762	arg1	performances					764:775	Growth performances	757:775	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR)	757:846	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	1	61	theme	growth	301:306	arg1	performances					308:319	broiler chickens' growth performances	283:319	broiler chickens' growth performances	283:319	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	2	62	theme	citrus	517:522	arg1	treatments					524:533	2 citrus treatments	515:533	2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively	515:658	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	6	63	theme	microbiota	1069:1078	arg1	analysis					1080:1087	microbiota analysis	1069:1087	microbiota analysis at d 7 and 42	1069:1101	One broiler chicken was randomly selected from each pen (10 chickens/group) and ceca samples were collected for microbiota analysis at d 7 and 42.
36881787	8	64	theme	major	1303:1307	arg1	component					1309:1317	a major component	1301:1317	a major component of the SNCE	1301:1329	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	8	64	theme	major	1303:1307	arg1	oligosaccharides					1275:1290	pectic oligosaccharides	1268:1290	pectic oligosaccharides (POS)	1268:1296	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	2	65	theme	SNCE	641:644	arg1	2,500 ppm					628:636	2,500 ppm	628:636	2,500 ppm	628:636	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	2	65	theme	SNCE	641:644	arg1	250 ppm					616:622	250 ppm	616:622	250 ppm	616:622	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	14	66	theme	effect	2040:2045	arg1	understanding					2010:2022	a better understanding	2001:2022	a better understanding of the observed effect on broiler chickens' growth performances	2001:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	3	67	theme	broiler	734:740	arg1	chickens					742:749	31 broiler chickens	731:749	31 broiler chickens each	731:754	Each dietary treatment was composed of 10 experimental units (pen) of 31 broiler chickens each.
36881787	4	68	theme	conversion	825:834	arg1	FCR					843:845	FCR	843:845	FCR	843:845	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	4	68	theme	conversion	825:834	arg1	ratio					836:840	feed conversion ratio	820:840	feed conversion ratio (FCR)	820:846	Growth performances such as feed consumption, body weight, and feed conversion ratio (FCR) were recorded weekly until d 42.
36881787	8	69	theme	SNCE	1326:1329	arg1	component					1309:1317	a major component	1301:1317	a major component of the SNCE	1301:1329	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	8	69	theme	SNCE	1326:1329	arg1	oligosaccharides					1275:1290	pectic oligosaccharides	1268:1290	pectic oligosaccharides (POS)	1268:1296	Results from the characterization of SNCE allowed to identify pectic oligosaccharides (POS) as a major component of the SNCE.
36881787	7	70	used	used	1133:1136	arg2	methods					1120:1126	Chromatographic methods	1104:1126	Chromatographic methods	1104:1126	Chromatographic methods were used to determine molecules that enter into the composition of the SNCE.
36881787	14	71	from	performances	2075:2086	arg1	understanding					2010:2022	a better understanding	2001:2022	a better understanding of the observed effect on broiler chickens' growth performances	2001:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	3	72	theme	chickens	742:749	arg1	pen					723:725	pen	723:725	pen	723:725	Each dietary treatment was composed of 10 experimental units (pen) of 31 broiler chickens each.
36881787	3	72	theme	chickens	742:749	arg1	units					716:720	10 experimental units	700:720	10 experimental units (pen) of 31 broiler chickens each	700:754	Each dietary treatment was composed of 10 experimental units (pen) of 31 broiler chickens each.
36881787	2	73	theme	broiler	464:470	arg1	chickens					472:479	broiler chickens	464:479	broiler chickens	464:479	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	10	74	theme	CTL	1598:1600	arg1	diets					1602:1606	the CTL diets	1594:1606	the CTL diets (P < 0.01)	1594:1617	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	10	74	theme	CTL	1598:1600	arg1	<					1611:1611	P < 0.01	1609:1616	P < 0.01	1609:1616	The experiment performed on broiler chickens showed that the final body weight of broiler chickens fed diets supplemented with SNCE was higher than those fed the CTL diets (P < 0.01).
36881787	2	75	theme	males	375:379	arg1	total					350:354	A total	348:354	A total of 930 one-day-old males	348:379	A total of 930 one-day-old males were randomly assigned to 3 dietary treatments: a control treatment (CTL) in which broiler chickens were fed with a standard diet and 2 citrus treatments in which broiler chickens were fed with the same standard diet supplemented with 250 ppm and 2,500 ppm of SNCE, respectively.
36881787	14	76	from	effect	2040:2045	arg1	performances					2075:2086	broiler chickens' growth performances	2050:2086	broiler chickens' growth performances	2050:2086	Thus, opening new horizons for a better understanding of the observed effect on broiler chickens' growth performances.
36881787	12	77	theme	cecal	1822:1826	arg1	microbiota					1828:1837	the cecal microbiota	1818:1837	the cecal microbiota of broiler chickens	1818:1857	Results indicate that SNCE allowed enhancing chickens' performances without any modulation of the cecal microbiota of broiler chickens.
36881787	11	78	theme	dietary	1691:1697	arg1	supplementation					1699:1713	the dietary supplementation	1687:1713	the dietary supplementation of SNCE	1687:1721	Broiler cecal microbiota was impacted by age (P < 0.01) but not by the dietary supplementation of SNCE.
36881787	1	79	theme	standardized	232:243	arg1	SNCE					269:272	SNCE	269:272	SNCE	269:272	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
36881787	1	79	theme	standardized	232:243	arg1	extract					260:266	a standardized natural citrus extract	230:266	a standardized natural citrus extract (SNCE)	230:273	The objective of this study was to investigate the effect and composition of a standardized natural citrus extract (SNCE) on both broiler chickens' growth performances and intestinal microbiota.
35149089	6	0	theme	PVA/ST/ZnO	1110:1119	arg1	films					1121:1125	The developed PVA/ST/ZnO films	1096:1125	The developed PVA/ST/ZnO films	1096:1125	The developed PVA/ST/ZnO films were tested for packaging fresh-cut carrot slices to prevent microbial infection and prolong their shelf life.
35149089	6	1	theme	packaging	1143:1151	arg1	slices					1170:1175	packaging fresh-cut carrot slices	1143:1175	packaging fresh-cut carrot slices	1143:1175	The developed PVA/ST/ZnO films were tested for packaging fresh-cut carrot slices to prevent microbial infection and prolong their shelf life.
35149089	1	2	theme	polyvinyl	189:197	arg1	film					223:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film	175:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials	175:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	1	3	theme	packaging	295:303	arg1	materials					305:313	multifunctional food packaging materials	274:313	multifunctional food packaging materials	274:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	0	4	theme	films	99:103	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide	0:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	6	5	theme	fresh-cut	1153:1161	arg1	slices					1170:1175	packaging fresh-cut carrot slices	1143:1175	packaging fresh-cut carrot slices	1143:1175	The developed PVA/ST/ZnO films were tested for packaging fresh-cut carrot slices to prevent microbial infection and prolong their shelf life.
35149089	7	6	from	prospects	1377:1385	arg1	field					1412:1416	active food packaging field	1390:1416	active food packaging field	1390:1416	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	1	7	theme	alcohol/starch	199:212	arg1	film					223:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film	175:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials	175:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	7	8	theme	PVA/ST/ZnO	1320:1329	arg1	films					1345:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	3	9	theme	nanocomposite	685:697	arg1	films					699:703	compact and dense nanocomposite films	667:703	compact and dense nanocomposite films	667:703	Results revealed that rod-like ZnO nanofillers could be uniformly distributed into the PVA/ST matrix, playing the role of compatibilizers to provide compact and dense nanocomposite films.
35149089	2	10	theme	ZnO	342:344	arg1	nanofillers					346:356	rod-like ZnO nanofillers	333:356	rod-like ZnO nanofillers	333:356	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	4	11	theme	virgin	828:833	arg1	film					842:845	virgin PVA/ST film	828:845	virgin PVA/ST film	828:845	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	0	12	theme	zinc	111:114	arg1	oxide					116:120	zinc oxide	111:120	zinc oxide	111:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	2	13	theme	rod-like	333:340	arg1	nanofillers					346:356	rod-like ZnO nanofillers	333:356	rod-like ZnO nanofillers	333:356	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	4	14	theme	barrier	794:800	arg1	properties					802:811	greatly improved mechanical and water vapor barrier properties	750:811	greatly improved mechanical and water vapor barrier properties	750:811	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	7	15	theme	developed	1271:1279	arg1	films					1345:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	5	16	with	endowed	883:889	arg1	activity					932:939	excellent antimicrobial activity	908:939	excellent antimicrobial activity against both E. coli and S. aureus	908:974	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	2	17	theme	water	446:450	arg1	performances					460:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	7	18	theme	multifunctional	1304:1318	arg1	films					1345:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	6	19	theme	shelf	1226:1230	arg1	life					1232:1235	their shelf life	1220:1235	their shelf life	1220:1235	The developed PVA/ST/ZnO films were tested for packaging fresh-cut carrot slices to prevent microbial infection and prolong their shelf life.
35149089	5	20	theme	meanwhile	1027:1035	arg1	capability					1016:1025	outstanding UV-shielding capability meanwhile	991:1035	outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%)	991:1093	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	6	21	theme	microbial	1188:1196	arg1	infection					1198:1206	microbial infection	1188:1206	microbial infection	1188:1206	The developed PVA/ST/ZnO films were tested for packaging fresh-cut carrot slices to prevent microbial infection and prolong their shelf life.
35149089	1	22	theme	PVA/ST	215:220	arg1	film					223:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film	175:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials	175:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	7	23	theme	broad	1359:1363	arg1	prospects					1377:1385	broad application prospects	1359:1385	broad application prospects in active food packaging field	1359:1416	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	2	24	theme	microstructural	365:379	arg1	performances					460:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	5	25	theme	PVA/ST	891:896	arg1	film					898:901	PVA/ST film	891:901	PVA/ST film	891:901	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	6	26	theme	carrot	1163:1168	arg1	slices					1170:1175	packaging fresh-cut carrot slices	1143:1175	packaging fresh-cut carrot slices	1143:1175	The developed PVA/ST/ZnO films were tested for packaging fresh-cut carrot slices to prevent microbial infection and prolong their shelf life.
35149089	0	27	theme	transparent	22:32	arg1	films					99:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films	15:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide	15:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	7	28	theme	active	1390:1395	arg1	field					1412:1416	active food packaging field	1390:1416	active food packaging field	1390:1416	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	4	29	theme	PVA/ST	835:840	arg1	film					842:845	virgin PVA/ST film	828:845	virgin PVA/ST film	828:845	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	5	30	theme	optical	1054:1060	arg1	%					1092:1092	approximately 90%	1076:1092	approximately 90%	1076:1092	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	5	30	theme	optical	1054:1060	arg1	transparency					1062:1073	highly optical transparency	1047:1073	highly optical transparency (approximately 90%)	1047:1093	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	2	31	theme	PVA/ST	476:481	arg1	films					493:497	PVA/ST composite films	476:497	PVA/ST composite films	476:497	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	2	32	theme	composite	483:491	arg1	films					493:497	PVA/ST composite films	476:497	PVA/ST composite films	476:497	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	2	33	theme	nanofillers	346:356	arg1	influence					320:328	The influence	316:328	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films	316:497	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	5	34	theme	antimicrobial	918:930	arg1	activity					932:939	excellent antimicrobial activity	908:939	excellent antimicrobial activity against both E. coli and S. aureus	908:974	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	0	35	theme	multifunctional	38:52	arg1	films					99:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films	15:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide	15:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	5	36	theme	outstanding	991:1001	arg1	capability					1016:1025	outstanding UV-shielding capability meanwhile	991:1035	outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%)	991:1093	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	4	37	theme	resulting	710:718	arg1	films					734:738	The resulting nanocomposite films	706:738	The resulting nanocomposite films	706:738	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	1	38	theme	rod-like	246:253	arg1	nanofillers					259:269	rod-like ZnO nanofillers	246:269	rod-like ZnO nanofillers as multifunctional food packaging materials	246:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	2	39	theme	films	493:497	arg1	influence					320:328	The influence	316:328	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films	316:497	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	5	40	theme	distributed	867:877	arg1	ZnO					879:881	the well distributed ZnO	858:881	the well distributed ZnO	858:881	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	1	41	theme	ZnO	255:257	arg1	nanofillers					259:269	rod-like ZnO nanofillers	246:269	rod-like ZnO nanofillers as multifunctional food packaging materials	246:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	0	42	theme	alcohol/starch	64:77	arg1	films					99:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films	15:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide	15:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	7	43	theme	application	1365:1375	arg1	prospects					1377:1385	broad application prospects	1359:1385	broad application prospects in active food packaging field	1359:1416	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	3	44	theme	ZnO	549:551	arg1	nanofillers					553:563	rod-like ZnO nanofillers	540:563	rod-like ZnO nanofillers	540:563	Results revealed that rod-like ZnO nanofillers could be uniformly distributed into the PVA/ST matrix, playing the role of compatibilizers to provide compact and dense nanocomposite films.
35149089	0	45	theme	polyvinyl	54:62	arg1	films					99:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films	15:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide	15:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	3	46	theme	compatibilizers	640:654	arg1	role					632:635	the role	628:635	the role of compatibilizers	628:654	Results revealed that rod-like ZnO nanofillers could be uniformly distributed into the PVA/ST matrix, playing the role of compatibilizers to provide compact and dense nanocomposite films.
35149089	7	47	theme	nanocomposite	1331:1343	arg1	films					1345:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	4	48	theme	vapor	788:792	arg1	properties					802:811	greatly improved mechanical and water vapor barrier properties	750:811	greatly improved mechanical and water vapor barrier properties	750:811	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	7	49	theme	food	1397:1400	arg1	field					1412:1416	active food packaging field	1390:1416	active food packaging field	1390:1416	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	3	50	theme	dense	679:683	arg1	films					699:703	compact and dense nanocomposite films	667:703	compact and dense nanocomposite films	667:703	Results revealed that rod-like ZnO nanofillers could be uniformly distributed into the PVA/ST matrix, playing the role of compatibilizers to provide compact and dense nanocomposite films.
35149089	2	51	dep	together	432:439	arg1	with					441:444	with	441:444	with	441:444	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	4	52	theme	improved	758:765	arg1	properties					802:811	greatly improved mechanical and water vapor barrier properties	750:811	greatly improved mechanical and water vapor barrier properties	750:811	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	2	53	dep	microstructural	365:379	arg1	mechanical					411:420	mechanical	411:420	mechanical	411:420	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	2	53	dep	microstructural	365:379	arg1	antibacterial					396:408	antibacterial	396:408	antibacterial	396:408	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	2	53	dep	microstructural	365:379	arg1	UV-shielding					382:393	UV-shielding	382:393	UV-shielding	382:393	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	2	53	dep	microstructural	365:379	arg1	thermal					423:429	thermal	423:429	thermal	423:429	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	2	54	from	influence	320:328	arg1	performances					460:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	3	55	theme	PVA/ST	605:610	arg1	matrix					612:617	the PVA/ST matrix	601:617	the PVA/ST matrix	601:617	Results revealed that rod-like ZnO nanofillers could be uniformly distributed into the PVA/ST matrix, playing the role of compatibilizers to provide compact and dense nanocomposite films.
35149089	7	56	contain	possess	1351:1357	arg2	prospects					1377:1385	broad application prospects	1359:1385	broad application prospects in active food packaging field	1359:1416	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	7	56	contain	possess	1351:1357	arg1	films					1345:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	3	57	theme	rod-like	540:547	arg1	nanofillers					553:563	rod-like ZnO nanofillers	540:563	rod-like ZnO nanofillers	540:563	Results revealed that rod-like ZnO nanofillers could be uniformly distributed into the PVA/ST matrix, playing the role of compatibilizers to provide compact and dense nanocomposite films.
35149089	5	58	theme	UV-shielding	1003:1014	arg1	capability					1016:1025	outstanding UV-shielding capability meanwhile	991:1035	outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%)	991:1093	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	0	59	theme	nanocomposite	85:97	arg1	films					99:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films	15:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide	15:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	2	60	theme	barrier	452:458	arg1	performances					460:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances	361:471	The influence of rod-like ZnO nanofillers on the microstructural, UV-shielding, antibacterial, mechanical, thermal, together with water barrier performances of PVA/ST composite films was fully studied.
35149089	3	61	theme	compact	667:673	arg1	films					699:703	compact and dense nanocomposite films	667:703	compact and dense nanocomposite films	667:703	Results revealed that rod-like ZnO nanofillers could be uniformly distributed into the PVA/ST matrix, playing the role of compatibilizers to provide compact and dense nanocomposite films.
35149089	5	62	theme	excellent	908:916	arg1	activity					932:939	excellent antimicrobial activity	908:939	excellent antimicrobial activity against both E. coli and S. aureus	908:974	Moreover, the well distributed ZnO endowed PVA/ST film with excellent antimicrobial activity against both E. coli and S. aureus, together with outstanding UV-shielding capability meanwhile retaining highly optical transparency (approximately 90%).
35149089	4	63	theme	mechanical	767:776	arg1	properties					802:811	greatly improved mechanical and water vapor barrier properties	750:811	greatly improved mechanical and water vapor barrier properties	750:811	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	4	64	theme	nanocomposite	720:732	arg1	films					734:738	The resulting nanocomposite films	706:738	The resulting nanocomposite films	706:738	The resulting nanocomposite films presented greatly improved mechanical and water vapor barrier properties as compared to virgin PVA/ST film.
35149089	1	65	theme	multifunctional	274:288	arg1	materials					305:313	multifunctional food packaging materials	274:313	multifunctional food packaging materials	274:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	0	66	theme	based	79:83	arg1	films					99:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films	15:103	highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide	15:120	Fabrication of highly transparent and multifunctional polyvinyl alcohol/starch based nanocomposite films using zinc oxide nanoparticles as compatibilizers.
35149089	6	67	theme	developed	1100:1108	arg1	films					1121:1125	The developed PVA/ST/ZnO films	1096:1125	The developed PVA/ST/ZnO films	1096:1125	The developed PVA/ST/ZnO films were tested for packaging fresh-cut carrot slices to prevent microbial infection and prolong their shelf life.
35149089	7	68	theme	packaging	1402:1410	arg1	field					1412:1416	active food packaging field	1390:1416	active food packaging field	1390:1416	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	1	69	theme	biodegradable	175:187	arg1	film					223:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film	175:226	biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials	175:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
35149089	7	70	theme	transparent	1288:1298	arg1	films					1345:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films	1267:1349	These results indicated that the developed highly transparent and multifunctional PVA/ST/ZnO nanocomposite films possess broad application prospects in active food packaging field.
35149089	1	71	theme	food	290:293	arg1	materials					305:313	multifunctional food packaging materials	274:313	multifunctional food packaging materials	274:313	This work explored biodegradable polyvinyl alcohol/starch (PVA/ST) film compatibilized by rod-like ZnO nanofillers as multifunctional food packaging materials.
36943661	5	0	theme	VTE	696:698	arg1	VTE					696:698	recurrent VTE	686:698	recurrent VTE	686:698	The outcome was a composite of recurrent VTE, major bleeding or clinically relevant non-major bleeding.
36943661	5	0	theme	VTE	696:698	arg1	outcome					659:665	The outcome	655:665	The outcome	655:665	The outcome was a composite of recurrent VTE, major bleeding or clinically relevant non-major bleeding.
36943661	5	0	theme	VTE	696:698	arg1	composite					673:681	a composite	671:681	a composite of recurrent VTE	671:698	The outcome was a composite of recurrent VTE, major bleeding or clinically relevant non-major bleeding.
36943661	5	0	theme	VTE	696:698	arg1	bleeding					749:756	bleeding	749:756	bleeding	749:756	The outcome was a composite of recurrent VTE, major bleeding or clinically relevant non-major bleeding.
36943661	5	0	theme	VTE	696:698	arg1	bleeding					707:714	bleeding	707:714	bleeding	707:714	The outcome was a composite of recurrent VTE, major bleeding or clinically relevant non-major bleeding.
36943661	6	1	theme	anticoagulation	835:849	arg1	group					851:855	each anticoagulation group	830:855	each anticoagulation group (LMWH, DOAC)	830:868	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	0	2	with	patients	30:37	arg1	malignancies					85:96	hematological malignancies	71:96	hematological malignancies	71:96	Direct oral anticoagulants in patients with venous thromboembolism and hematological malignancies.
36943661	0	2	with	patients	30:37	arg1	thromboembolism					51:65	venous thromboembolism	44:65	venous thromboembolism	44:65	Direct oral anticoagulants in patients with venous thromboembolism and hematological malignancies.
36943661	9	3	theme	outcome	1204:1210	arg1	incidence					1177:1185	The 12-month cumulative incidence	1153:1185	The 12-month cumulative incidence of the composite outcome	1153:1210	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	3	theme	outcome	1204:1210	arg1	%					1220:1220	24.2%	1216:1220	24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group	1216:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	8	4	theme	cell	1123:1126	arg1	dyscrasia					1128:1136	plasma cell dyscrasia	1116:1136	plasma cell dyscrasia in 32 (22.3%)	1116:1150	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	10	5	theme	recurrent	1344:1352	arg1	VTE					1354:1356	Two recurrent VTE	1340:1356	Two recurrent VTE	1340:1356	Two recurrent VTE occurred (both in the DOAC group while off-treatment).
36943661	9	6	theme	CI	1227:1228	arg1	%					1239:1239	95% CI 15.9-33.5%	1223:1239	95% CI 15.9-33.5%	1223:1239	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	6	theme	CI	1227:1228	arg1	%					1220:1220	24.2%	1216:1220	24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group	1216:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	12	7	theme	safe	1588:1591	arg1	alternative					1607:1617	a safe and effective alternative	1586:1617	a safe and effective alternative to LMWH for VTE	1586:1633	This study generates the hypothesis that DOACs may be a safe and effective alternative to LMWH for VTE in patients with HM types represented in the study.
36943661	12	7	theme	safe	1588:1591	arg1	DOACs					1573:1577	DOACs	1573:1577	DOACs	1573:1577	This study generates the hypothesis that DOACs may be a safe and effective alternative to LMWH for VTE in patients with HM types represented in the study.
36943661	4	8	theme	cohort	511:516	arg1	study					518:522	a retrospective cohort study	495:522	a retrospective cohort study	495:522	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	4	8	theme	cohort	511:516	arg1	This					487:490	This	487:490	This	487:490	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	9	9	from	%	1276:1276	arg1	HR					1316:1317	HR 1.51 [0.695-3.297]	1316:1336	HR 1.51 [0.695-3.297]	1316:1336	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	9	from	%	1276:1276	arg1	group					1309:1313	the DOAC group	1300:1313	the DOAC group (HR 1.51 [0.695-3.297])	1300:1337	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	9	from	%	1276:1276	arg1	group					1262:1266	the LMWH group	1253:1266	the LMWH group	1253:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	10	10	dep	group	1385:1389	arg1	both					1368:1371	both	1368:1371	both	1368:1371	Two recurrent VTE occurred (both in the DOAC group while off-treatment).
36943661	6	11	dep	group	851:855	arg1	DOAC					864:867	DOAC	864:867	DOAC	864:867	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	6	11	dep	group	851:855	arg1	LMWH					858:861	LMWH	858:861	LMWH	858:861	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	9	12	theme	LMWH	1257:1260	arg1	group					1262:1266	the LMWH group	1253:1266	the LMWH group	1253:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	7	13	theme	acute	1036:1040	arg1	VTE					1042:1044	acute VTE	1036:1044	acute VTE	1036:1044	143 HM patients treated with LMWH (96) or DOACs (47) for acute VTE were included.
36943661	9	14	dep	%	1287:1287	arg1	n = 8					1290:1294	n = 8	1290:1294	8.5-31.5%; n = 8	1279:1294	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	7	15	theme	HM	983:984	arg1	patients					986:993	143 HM patients	979:993	143 HM patients treated with LMWH (96) or DOACs (47) for acute VTE	979:1044	143 HM patients treated with LMWH (96) or DOACs (47) for acute VTE were included.
36943661	4	16	theme	newly-diagnosed	562:576	arg1	VTE					578:580	newly-diagnosed VTE	562:580	newly-diagnosed VTE	562:580	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	6	17	theme	hazards	932:938	arg1	model					940:944	cox-proportional hazards model	915:944	cox-proportional hazards model	915:944	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	9	18	theme	%	1225:1225	arg1	%					1239:1239	95% CI 15.9-33.5%	1223:1239	95% CI 15.9-33.5%	1223:1239	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	18	theme	%	1225:1225	arg1	%					1220:1220	24.2%	1216:1220	24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group	1216:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	12	19	with	patients	1638:1645	arg1	types					1655:1659	HM types	1652:1659	HM types represented in the study	1652:1684	This study generates the hypothesis that DOACs may be a safe and effective alternative to LMWH for VTE in patients with HM types represented in the study.
36943661	10	20	theme	DOAC	1380:1383	arg1	group					1385:1389	the DOAC group	1376:1389	the DOAC group while off-treatment	1376:1409	Two recurrent VTE occurred (both in the DOAC group while off-treatment).
36943661	3	21	theme	acute	476:480	arg1	VTE					482:484	acute VTE	476:484	acute VTE	476:484	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	3	22	theme	HM	402:403	arg1	patients					405:412	HM patients	402:412	HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE	402:484	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	0	23	theme	oral	7:10	arg1	anticoagulants					12:25	oral anticoagulants	7:25	oral anticoagulants	7:25	Direct oral anticoagulants in patients with venous thromboembolism and hematological malignancies.
36943661	12	24	theme	HM	1652:1653	arg1	types					1655:1659	HM types	1652:1659	HM types represented in the study	1652:1684	This study generates the hypothesis that DOACs may be a safe and effective alternative to LMWH for VTE in patients with HM types represented in the study.
36943661	11	25	theme	DOAC-treated	1487:1498	arg1	HR					1509:1510	HR 4.85 [0.64-36.56]	1509:1528	HR 4.85 [0.64-36.56]	1509:1528	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	11	25	theme	DOAC-treated	1487:1498	arg1	%					1484:1484	2.1%	1481:1484	2.1%	1481:1484	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	11	25	theme	DOAC-treated	1487:1498	arg1	patient					1500:1506	1 (2.1%) DOAC-treated patient	1478:1506	1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56])	1478:1529	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	1	26	theme	venous	174:179	arg1	VTE					198:200	VTE	198:200	VTE	198:200	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	1	26	theme	venous	174:179	arg1	thromboembolism					181:195	venous thromboembolism	174:195	venous thromboembolism (VTE)	174:201	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	6	27	dep	incidence	770:778	arg1	interval					796:803	[95% confidence interval	780:803	Cumulative incidence [95% confidence interval (CI)]	759:809	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	6	27	dep	incidence	770:778	arg1	CI					806:807	CI	806:807	CI	806:807	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	7	28	theme	143	979:981	arg1	patients					986:993	143 HM patients	979:993	143 HM patients treated with LMWH (96) or DOACs (47) for acute VTE	979:1044	143 HM patients treated with LMWH (96) or DOACs (47) for acute VTE were included.
36943661	8	29	from	dyscrasia	1128:1136	arg1	%					1149:1149	22.3%	1145:1149	22.3%	1145:1149	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	29	from	dyscrasia	1128:1136	arg1	83					1103:1104	83	1103:1104	83	1103:1104	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	29	from	dyscrasia	1128:1136	arg1	%					1109:1109	58%	1107:1109	58%	1107:1109	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	29	from	dyscrasia	1128:1136	arg1	32					1141:1142	32	1141:1142	32	1141:1142	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	9	30	dep	%	1276:1276	arg1	%					1287:1287	8.5-31.5%	1279:1287	8.5-31.5%; n = 8	1279:1294	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	3	31	theme	bleeding	390:397	arg1	aim					336:338	Out aim	332:338	Out aim	332:338	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	3	31	theme	bleeding	390:397	arg1	incidence					358:366	the incidence	354:366	the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE	354:484	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	6	32	theme	hazard	874:879	arg1	HR					889:890	HR	889:890	HR	889:890	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	6	32	theme	hazard	874:879	arg1	ratios					881:886	hazard ratios	874:886	hazard ratios (HR)	874:891	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	11	33	contain	had	1447:1449	arg1	%					1422:1422	9.4%	1419:1422	9.4%	1419:1422	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	11	33	contain	had	1447:1449	arg1	patients					1438:1445	Nine (9.4%) LMWH-treated patients	1413:1445	Nine (9.4%) LMWH-treated patients	1413:1445	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	11	33	contain	had	1447:1449	arg2	bleeding					1457:1464	bleeding	1457:1464	bleeding	1457:1464	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	4	34	theme	retrospective	497:509	arg1	study					518:522	a retrospective cohort study	495:522	a retrospective cohort study	495:522	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	4	34	theme	retrospective	497:509	arg1	This					487:490	This	487:490	This	487:490	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	9	35	from	%	1220:1220	arg1	HR					1316:1317	HR 1.51 [0.695-3.297]	1316:1336	HR 1.51 [0.695-3.297]	1316:1336	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	35	from	%	1220:1220	arg1	group					1309:1313	the DOAC group	1300:1313	the DOAC group (HR 1.51 [0.695-3.297])	1300:1337	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	35	from	%	1220:1220	arg1	group					1262:1266	the LMWH group	1253:1266	the LMWH group	1253:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	11	36	theme	LMWH-treated	1425:1436	arg1	%					1422:1422	9.4%	1419:1422	9.4%	1419:1422	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	11	36	theme	LMWH-treated	1425:1436	arg1	patients					1438:1445	Nine (9.4%) LMWH-treated patients	1413:1445	Nine (9.4%) LMWH-treated patients	1413:1445	Nine (9.4%) LMWH-treated patients had major bleeding compared to 1 (2.1%) DOAC-treated patient (HR 4.85 [0.64-36.56]).
36943661	2	37	theme	control	278:284	arg1	group					286:290	a control group	276:290	a control group	276:290	Retrospective studies to date lacked a control group and did not focus on patients with VTE.
36943661	3	38	theme	low	427:429	arg1	heparin					448:454	low molecular weight heparin	427:454	low molecular weight heparin (LMWH)	427:461	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	3	38	theme	low	427:429	arg1	LMWH					457:460	LMWH	457:460	LMWH	457:460	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	3	39	theme	VTE	371:373	arg1	recurrence					375:384	VTE recurrence	371:384	VTE recurrence	371:384	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	4	40	dep	study	518:522	arg1	indexed					583:589	indexed	583:589	indexed on the first day of anticoagulation	583:625	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	4	40	dep	study	518:522	arg1	followed					631:638	followed	631:638	followed for 12 months	631:652	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	12	41	from	alternative	1607:1617	arg1	patients					1638:1645	patients	1638:1645	patients with HM types represented in the study	1638:1684	This study generates the hypothesis that DOACs may be a safe and effective alternative to LMWH for VTE in patients with HM types represented in the study.
36943661	9	42	theme	DOAC	1304:1307	arg1	HR					1316:1317	HR 1.51 [0.695-3.297]	1316:1336	HR 1.51 [0.695-3.297]	1316:1336	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	42	theme	DOAC	1304:1307	arg1	group					1309:1313	the DOAC group	1300:1313	the DOAC group (HR 1.51 [0.695-3.297])	1300:1337	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	1	43	theme	thromboembolism	181:195	arg1	treatment					161:169	the treatment	157:169	the treatment of venous thromboembolism (VTE) in hematological malignancies (HM)	157:236	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	3	44	theme	recurrence	375:384	arg1	aim					336:338	Out aim	332:338	Out aim	332:338	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	3	44	theme	recurrence	375:384	arg1	incidence					358:366	the incidence	354:366	the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE	354:484	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	8	45	theme	plasma	1116:1121	arg1	dyscrasia					1128:1136	plasma cell dyscrasia	1116:1136	plasma cell dyscrasia in 32 (22.3%)	1116:1150	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	2	46	with	patients	313:320	arg1	VTE					327:329	VTE	327:329	VTE	327:329	Retrospective studies to date lacked a control group and did not focus on patients with VTE.
36943661	10	47	dep	occurred	1358:1365	arg1	group					1385:1389	the DOAC group	1376:1389	the DOAC group while off-treatment	1376:1409	Two recurrent VTE occurred (both in the DOAC group while off-treatment).
36943661	8	48	from	lymphoma	1091:1098	arg1	%					1149:1149	22.3%	1145:1149	22.3%	1145:1149	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	48	from	lymphoma	1091:1098	arg1	83					1103:1104	83	1103:1104	83	1103:1104	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	48	from	lymphoma	1091:1098	arg1	%					1109:1109	58%	1107:1109	58%	1107:1109	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	48	from	lymphoma	1091:1098	arg1	32					1141:1142	32	1141:1142	32	1141:1142	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	49	theme	common	1070:1075	arg1	lymphoma					1091:1098	lymphoma	1091:1098	lymphoma in 83 (58%)	1091:1110	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	49	theme	common	1070:1075	arg1	types					1080:1084	The most common HM types	1061:1084	The most common HM types	1061:1084	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	6	50	theme	confidence	785:794	arg1	interval					796:803	[95% confidence interval	780:803	Cumulative incidence [95% confidence interval (CI)]	759:809	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	6	50	theme	confidence	785:794	arg1	CI					806:807	CI	806:807	CI	806:807	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	1	51	theme	direct	118:123	arg1	anticoagulants					130:143	direct oral anticoagulants	118:143	direct oral anticoagulants (DOACs)	118:151	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	1	51	theme	direct	118:123	arg1	DOACs					146:150	DOACs	146:150	DOACs	146:150	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	0	52	theme	venous	44:49	arg1	thromboembolism					51:65	venous thromboembolism	44:65	venous thromboembolism	44:65	Direct oral anticoagulants in patients with venous thromboembolism and hematological malignancies.
36943661	9	53	theme	12-month	1157:1164	arg1	incidence					1177:1185	The 12-month cumulative incidence	1153:1185	The 12-month cumulative incidence of the composite outcome	1153:1210	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	53	theme	12-month	1157:1164	arg1	%					1220:1220	24.2%	1216:1220	24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group	1216:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	6	54	theme	%	783:783	arg1	interval					796:803	[95% confidence interval	780:803	Cumulative incidence [95% confidence interval (CI)]	759:809	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	6	54	theme	%	783:783	arg1	CI					806:807	CI	806:807	CI	806:807	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	6	55	theme	[95	780:782	arg1	%					783:783	%	783:783	%	783:783	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	1	56	theme	oral	125:128	arg1	anticoagulants					130:143	direct oral anticoagulants	118:143	direct oral anticoagulants (DOACs)	118:151	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	1	56	theme	oral	125:128	arg1	DOACs					146:150	DOACs	146:150	DOACs	146:150	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	12	57	theme	effective	1597:1605	arg1	alternative					1607:1617	a safe and effective alternative	1586:1617	a safe and effective alternative to LMWH for VTE	1586:1633	This study generates the hypothesis that DOACs may be a safe and effective alternative to LMWH for VTE in patients with HM types represented in the study.
36943661	12	57	theme	effective	1597:1605	arg1	DOACs					1573:1577	DOACs	1573:1577	DOACs	1573:1577	This study generates the hypothesis that DOACs may be a safe and effective alternative to LMWH for VTE in patients with HM types represented in the study.
36943661	9	58	theme	cumulative	1166:1175	arg1	incidence					1177:1185	The 12-month cumulative incidence	1153:1185	The 12-month cumulative incidence of the composite outcome	1153:1210	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	9	58	theme	cumulative	1166:1175	arg1	%					1220:1220	24.2%	1216:1220	24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group	1216:1266	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	4	59	theme	first	598:602	arg1	day					604:606	the first day	594:606	the first day of anticoagulation	594:625	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	4	60	with	patients	534:541	arg1	HM					555:556	active HM	548:556	active HM	548:556	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	4	60	with	patients	534:541	arg1	VTE					578:580	newly-diagnosed VTE	562:580	newly-diagnosed VTE	562:580	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	3	61	theme	molecular	431:439	arg1	heparin					448:454	low molecular weight heparin	427:454	low molecular weight heparin (LMWH)	427:461	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	3	61	theme	molecular	431:439	arg1	LMWH					457:460	LMWH	457:460	LMWH	457:460	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	1	62	theme	hematological	206:218	arg1	HM					234:235	HM	234:235	HM	234:235	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	1	62	theme	hematological	206:218	arg1	malignancies					220:231	hematological malignancies	206:231	hematological malignancies (HM)	206:236	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	8	63	theme	HM	1077:1078	arg1	lymphoma					1091:1098	lymphoma	1091:1098	lymphoma in 83 (58%)	1091:1110	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	8	63	theme	HM	1077:1078	arg1	types					1080:1084	The most common HM types	1061:1084	The most common HM types	1061:1084	The most common HM types were lymphoma in 83 (58%) and plasma cell dyscrasia in 32 (22.3%).
36943661	2	64	theme	Retrospective	239:251	arg1	studies					253:259	Retrospective studies	239:259	Retrospective studies to date	239:267	Retrospective studies to date lacked a control group and did not focus on patients with VTE.
36943661	3	65	theme	weight	441:446	arg1	heparin					448:454	low molecular weight heparin	427:454	low molecular weight heparin (LMWH)	427:461	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	3	65	theme	weight	441:446	arg1	LMWH					457:460	LMWH	457:460	LMWH	457:460	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	6	66	theme	cox-proportional	915:930	arg1	model					940:944	cox-proportional hazards model	915:944	cox-proportional hazards model	915:944	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	0	67	theme	hematological	71:83	arg1	malignancies					85:96	hematological malignancies	71:96	hematological malignancies	71:96	Direct oral anticoagulants in patients with venous thromboembolism and hematological malignancies.
36943661	6	68	theme	Cumulative	759:768	arg1	incidence					770:778	Cumulative incidence [95% confidence interval (CI)]	759:809	Cumulative incidence [95% confidence interval (CI)]	759:809	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	4	69	theme	anticoagulation	611:625	arg1	day					604:606	the first day	594:606	the first day of anticoagulation	594:625	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	6	70	theme	competing	963:971	arg1	risk					973:976	a competing risk	961:976	a competing risk	961:976	Cumulative incidence [95% confidence interval (CI)] was calculated for each anticoagulation group (LMWH, DOAC) and hazard ratios (HR) were calculated using cox-proportional hazards model, with death as a competing risk.
36943661	5	71	theme	recurrent	686:694	arg1	VTE					696:698	recurrent VTE	686:698	recurrent VTE	686:698	The outcome was a composite of recurrent VTE, major bleeding or clinically relevant non-major bleeding.
36943661	3	72	from	incidence	358:366	arg1	patients					405:412	HM patients	402:412	HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE	402:484	Out aim was to assess the incidence of VTE recurrence and bleeding in HM patients treated with low molecular weight heparin (LMWH) or DOACs for acute VTE.
36943661	9	73	dep	%	1239:1239	arg1	n = 22					1242:1247	n = 22	1242:1247	n = 22	1242:1247	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
36943661	1	74	from	treatment	161:169	arg1	HM					234:235	HM	234:235	HM	234:235	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	1	74	from	treatment	161:169	arg1	malignancies					220:231	hematological malignancies	206:231	hematological malignancies (HM)	206:236	Data are needed on direct oral anticoagulants (DOACs) for the treatment of venous thromboembolism (VTE) in hematological malignancies (HM).
36943661	4	75	theme	active	548:553	arg1	HM					555:556	active HM	548:556	active HM	548:556	This is a retrospective cohort study including patients with active HM and newly-diagnosed VTE, indexed on the first day of anticoagulation and followed for 12 months.
36943661	9	76	theme	composite	1194:1202	arg1	outcome					1204:1210	the composite outcome	1190:1210	the composite outcome	1190:1210	The 12-month cumulative incidence of the composite outcome was 24.2% (95% CI 15.9-33.5%; n = 22) in the LMWH group and 18.5% (8.5-31.5%; n = 8) in the DOAC group (HR 1.51 [0.695-3.297]).
35288311	3	0	theme	endothelial	493:503	arg1	HUVECs					512:517	HUVECs	512:517	HUVECs	512:517	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	0	theme	endothelial	493:503	arg1	cells					505:509	human umbilical vein endothelial cells	472:509	human umbilical vein endothelial cells (HUVECs)	472:518	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	15	1	theme	medical	2517:2523	arg1	management					2525:2534	the medical management	2513:2534	the medical management of hair loss	2513:2547	The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss.
35288311	1	2	theme	hair	256:259	arg1	neogenesis					261:270	hair neogenesis	256:270	hair neogenesis	256:270	Hair follicle (HF) regeneration remains challenging, principally due to the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis.
35288311	1	3	theme	Hair	96:99	arg1	follicle					101:108	Hair follicle	96:108	Hair follicle (HF) regeneration	96:126	Hair follicle (HF) regeneration remains challenging, principally due to the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis.
35288311	1	3	theme	Hair	96:99	arg1	HF					111:112	HF	111:112	HF	111:112	Hair follicle (HF) regeneration remains challenging, principally due to the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis.
35288311	3	4	theme	dermal	521:526	arg1	cells					536:540	dermal papilla cells	521:540	dermal papilla cells (DPCs)	521:547	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	4	theme	dermal	521:526	arg1	DPCs					543:546	DPCs	543:546	DPCs	543:546	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	10	5	contain	have	1617:1620	arg2	applications					1630:1641	broader applications	1622:1641	broader applications	1622:1641	This method may also have broader applications in skin tissue (and appendage) engineering.
35288311	10	5	contain	have	1617:1620	arg1	method					1601:1606	This method	1596:1606	This method	1596:1606	This method may also have broader applications in skin tissue (and appendage) engineering.
35288311	3	6	theme	alginate	642:649	arg1	mixture					619:625	a mixture	617:625	a mixture of gelatin and alginate	617:649	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	12	7	theme	biomedical	1927:1936	arg1	applications					1938:1949	other biomedical applications	1921:1949	other biomedical applications	1921:1949	Three-dimensional (3D) bioprinting has become a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications.
35288311	14	8	theme	relevant	2313:2320	arg1	matrix					2336:2341	a physiologically relevant extracellular matrix	2295:2341	a physiologically relevant extracellular matrix	2295:2341	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	2	9	theme	bioprinting	299:309	arg1	technique					311:319	a 3D bioprinting technique	294:319	a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo	294:450	Here, we demonstrate a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo.
35288311	5	10	dep	1.2-fold	984:991	arg1	P < 0.05					994:1001	P < 0.05	994:1001	1.2-fold; P < 0.05	984:1001	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	15	11	contain	have	2484:2487	arg2	impact					2503:2508	a significant impact	2489:2508	a significant impact	2489:2508	The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss.
35288311	15	11	contain	have	2484:2487	arg1	ability					2444:2450	The ability to regenerate entire HFs	2440:2475	The ability to regenerate entire HFs	2440:2475	The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss.
35288311	11	12	theme	challenging	1734:1744	arg1	problem					1755:1761	a challenging clinical problem	1732:1761	a challenging clinical problem that influences quality of life	1732:1793	STATEMENT OF SIGNIFICANCE: Hair loss remains a challenging clinical problem that influences quality of life.
35288311	11	13	dep	STATEMENT	1687:1695	arg1	remains					1724:1730	remains	1724:1730	remains a challenging clinical problem that influences quality of life	1724:1793	STATEMENT OF SIGNIFICANCE: Hair loss remains a challenging clinical problem that influences quality of life.
35288311	9	14	theme	3D	1423:1424	arg1	scaffolds					1434:1442	Our 3D printed scaffolds	1419:1442	Our 3D printed scaffolds	1419:1442	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	5	15	dep	DPCs	978:981	arg1	1.2-fold					984:991	1.2-fold	984:991	1.2-fold; P < 0.05	984:1001	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	13	16	theme	cuticular	2090:2098	arg1	layers					2111:2116	cuticular and corium layers	2090:2116	cuticular and corium layers	2090:2116	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	3	17	theme	epidermal	554:562	arg1	EPCs					571:574	EPCs	571:574	EPCs	571:574	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	17	theme	epidermal	554:562	arg1	cells					564:568	epidermal cells	554:568	epidermal cells (EPCs)	554:575	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	4	18	theme	nude	860:863	arg1	mice					865:868	nude mice	860:868	nude mice	860:868	The bioprinted scaffold with epidermis- and dermis-like structure was subsequently transplanted into full-thickness wounds in nude mice.
35288311	8	19	theme	DPCs	1413:1416	arg1	bioprinting					1398:1408	the dot bioprinting	1390:1408	the dot bioprinting of DPCs	1390:1416	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	8	19	theme	DPCs	1413:1416	arg1	structure					1360:1368	the hierarchical grid structure	1338:1368	the hierarchical grid structure of the scaffold	1338:1384	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	5	20	theme	multilayer	875:884	arg1	scaffold					886:893	The multilayer scaffold	871:893	The multilayer scaffold	871:893	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	8	21	theme	appropriate	1271:1281	arg1	orientation					1283:1293	the appropriate orientation	1267:1293	the appropriate orientation	1267:1293	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	11	22	theme	SIGNIFICANCE	1700:1711	arg1	STATEMENT					1687:1695	STATEMENT	1687:1695	STATEMENT OF SIGNIFICANCE: Hair loss remains a challenging clinical problem that influences quality of life.	1687:1794	STATEMENT OF SIGNIFICANCE: Hair loss remains a challenging clinical problem that influences quality of life.
35288311	13	23	theme	multi-layer	2054:2064	arg1	scaffold					2076:2083	a multi-layer composite scaffold	2052:2083	a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body	2052:2198	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	9	24	from	contribution	1543:1554	arg1	management					1571:1580	the medical management	1559:1580	the medical management of hair loss	1559:1593	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	3	25	theme	scaffold	724:731	arg1	layers					702:707	the different layers	688:707	the different layers of a composite scaffold	688:731	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	0	26	theme	hair	68:71	arg1	regeneration					82:93	tissue-engineered hair follicle regeneration	50:93	tissue-engineered hair follicle regeneration	50:93	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration.
35288311	4	27	from	wounds	850:855	arg1	mice					865:868	nude mice	860:868	nude mice	860:868	The bioprinted scaffold with epidermis- and dermis-like structure was subsequently transplanted into full-thickness wounds in nude mice.
35288311	14	28	theme	self-aggregating	2257:2272	arg1	spheroids					2274:2282	self-aggregating spheroids	2257:2282	self-aggregating spheroids of DPCs	2257:2290	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	11	29	theme	Hair	1714:1717	arg1	loss					1719:1722	Hair loss	1714:1722	Hair loss	1714:1722	STATEMENT OF SIGNIFICANCE: Hair loss remains a challenging clinical problem that influences quality of life.
35288311	13	30	theme	bioprinting	1980:1990	arg1	technique					1992:2000	a 3D bioprinting technique	1975:2000	a 3D bioprinting technique based on a gelatin/alginate hydrogel	1975:2037	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	15	31	theme	significant	2491:2501	arg1	impact					2503:2508	a significant impact	2489:2508	a significant impact	2489:2508	The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss.
35288311	5	32	theme	proliferation	953:965	arg1	ability					967:973	increased the proliferation ability	939:973	increased the proliferation ability of DPCs (1.2-fold; P < 0.05)	939:1002	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	2	33	theme	multilayer	379:388	arg1	scaffold					400:407	a multilayer composite scaffold	377:407	a multilayer composite scaffold simulating the HF microenvironment	377:442	Here, we demonstrate a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo.
35288311	4	34	theme	bioprinted	738:747	arg1	scaffold					749:756	The bioprinted scaffold	734:756	The bioprinted scaffold with epidermis- and dermis-like structure	734:798	The bioprinted scaffold with epidermis- and dermis-like structure was subsequently transplanted into full-thickness wounds in nude mice.
35288311	6	35	theme	DPC	1059:1061	arg1	spheroids					1063:1071	self-aggregating DPC spheroids	1042:1071	self-aggregating DPC spheroids	1042:1071	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	0	36	theme	3D	0:1	arg1	bioprinting					3:13	3D bioprinting	0:13	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration	0:93	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration.
35288311	1	37	theme	follicle	101:108	arg1	regeneration					115:126	Hair follicle (HF) regeneration	96:126	Hair follicle (HF) regeneration	96:126	Hair follicle (HF) regeneration remains challenging, principally due to the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis.
35288311	8	38	theme	hierarchical	1342:1353	arg1	structure					1360:1368	the hierarchical grid structure	1338:1368	the hierarchical grid structure of the scaffold	1338:1384	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	5	39	theme	increased	939:947	arg1	ability					967:973	increased the proliferation ability	939:973	increased the proliferation ability of DPCs (1.2-fold; P < 0.05)	939:1002	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	13	40	with	scaffold	2076:2083	arg1	layers					2111:2116	cuticular and corium layers	2090:2116	cuticular and corium layers	2090:2116	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	10	41	theme	broader	1622:1628	arg1	applications					1630:1641	broader applications	1622:1641	broader applications	1622:1641	This method may also have broader applications in skin tissue (and appendage) engineering.
35288311	0	42	theme	gelatin-alginate	20:35	arg1	hydrogel					37:44	a gelatin-alginate hydrogel	18:44	a gelatin-alginate hydrogel	18:44	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration.
35288311	13	43	theme	papilla	2161:2167	arg1	DPCs					2176:2179	DPCs	2176:2179	DPCs	2176:2179	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	13	43	theme	papilla	2161:2167	arg1	cells					2169:2173	dermal papilla cells	2154:2173	dermal papilla cells (DPCs)	2154:2180	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	14	44	theme	HF	2418:2419	arg1	formation					2421:2429	HF formation	2418:2429	HF formation	2418:2429	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	3	45	theme	human	472:476	arg1	HUVECs					512:517	HUVECs	512:517	HUVECs	512:517	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	45	theme	human	472:476	arg1	cells					505:509	human umbilical vein endothelial cells	472:509	human umbilical vein endothelial cells (HUVECs)	472:518	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	6	46	theme	hair	1112:1115	arg1	induction					1117:1125	hair induction	1112:1125	hair induction (ALP, β-catenin, and α-SMA)	1112:1153	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	13	47	theme	3D	1977:1978	arg1	technique					1992:2000	a 3D bioprinting technique	1975:2000	a 3D bioprinting technique based on a gelatin/alginate hydrogel	1975:2037	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	9	48	theme	hair	1585:1588	arg1	loss					1590:1593	hair loss	1585:1593	hair loss	1585:1593	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	6	49	dep	induction	1117:1125	arg1	β-catenin					1133:1141	β-catenin	1133:1141	β-catenin	1133:1141	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	6	49	dep	induction	1117:1125	arg1	α-SMA					1148:1152	α-SMA	1148:1152	α-SMA	1148:1152	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	6	49	dep	induction	1117:1125	arg1	ALP					1128:1130	ALP	1128:1130	ALP	1128:1130	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	1	50	theme	microenvironmental	229:246	arg1	cues					248:251	the microenvironmental cues	225:251	the microenvironmental cues of hair neogenesis	225:270	Hair follicle (HF) regeneration remains challenging, principally due to the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis.
35288311	6	51	theme	DPC	1086:1088	arg1	genes					1090:1094	DPC genes	1086:1094	DPC genes associated with hair induction (ALP, β-catenin, and α-SMA)	1086:1153	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	12	52	dep	Three-dimensional	1796:1812	arg1	3D					1815:1816	3D	1815:1816	3D	1815:1816	Three-dimensional (3D) bioprinting has become a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications.
35288311	14	53	theme	epidermal-mesenchymal	2365:2385	arg1	interactions					2387:2398	epidermal-mesenchymal interactions	2365:2398	epidermal-mesenchymal interactions	2365:2398	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	3	54	theme	vein	488:491	arg1	HUVECs					512:517	HUVECs	512:517	HUVECs	512:517	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	54	theme	vein	488:491	arg1	cells					505:509	human umbilical vein endothelial cells	472:509	human umbilical vein endothelial cells (HUVECs)	472:518	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	15	55	theme	hair	2539:2542	arg1	loss					2544:2547	hair loss	2539:2547	hair loss	2539:2547	The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss.
35288311	13	56	theme	corium	2104:2109	arg1	layers					2111:2116	cuticular and corium layers	2090:2116	cuticular and corium layers	2090:2116	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	7	57	theme	immature	1220:1227	arg1	HFs					1229:1231	immature HFs	1220:1231	immature HFs	1220:1231	The dermal and epidermal cells self-assembled successfully into immature HFs in vitro.
35288311	1	58	theme	neogenesis	261:270	arg1	cues					248:251	the microenvironmental cues	225:251	the microenvironmental cues of hair neogenesis	225:270	Hair follicle (HF) regeneration remains challenging, principally due to the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis.
35288311	4	59	theme	full-thickness	835:848	arg1	wounds					850:855	full-thickness wounds	835:855	full-thickness wounds in nude mice	835:868	The bioprinted scaffold with epidermis- and dermis-like structure was subsequently transplanted into full-thickness wounds in nude mice.
35288311	2	60	theme	gelatin/alginate	332:347	arg1	GAH					359:361	GAH	359:361	GAH	359:361	Here, we demonstrate a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo.
35288311	2	60	theme	gelatin/alginate	332:347	arg1	hydrogel					349:356	a gelatin/alginate hydrogel	330:356	a gelatin/alginate hydrogel (GAH)	330:362	Here, we demonstrate a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo.
35288311	4	61	theme	dermis-like	778:788	arg1	structure					790:798	dermis-like structure	778:798	dermis-like structure	778:798	The bioprinted scaffold with epidermis- and dermis-like structure was subsequently transplanted into full-thickness wounds in nude mice.
35288311	14	62	theme	extracellular	2322:2334	arg1	matrix					2336:2341	a physiologically relevant extracellular matrix	2295:2341	a physiologically relevant extracellular matrix	2295:2341	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	12	63	theme	other	1921:1925	arg1	applications					1938:1949	other biomedical applications	1921:1949	other biomedical applications	1921:1949	Three-dimensional (3D) bioprinting has become a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications.
35288311	3	64	theme	different	692:700	arg1	layers					702:707	the different layers	688:707	the different layers of a composite scaffold	688:731	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	65	theme	papilla	528:534	arg1	cells					536:540	dermal papilla cells	521:540	dermal papilla cells (DPCs)	521:547	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	65	theme	papilla	528:534	arg1	DPCs					543:546	DPCs	543:546	DPCs	543:546	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	1	66	theme	platform	185:192	arg1	absence					172:178	the absence	168:178	the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis	168:270	Hair follicle (HF) regeneration remains challenging, principally due to the absence of a platform that can successfully generate the microenvironmental cues of hair neogenesis.
35288311	11	67	theme	clinical	1746:1753	arg1	problem					1755:1761	a challenging clinical problem	1732:1761	a challenging clinical problem that influences quality of life	1732:1793	STATEMENT OF SIGNIFICANCE: Hair loss remains a challenging clinical problem that influences quality of life.
35288311	9	68	theme	printed	1426:1432	arg1	scaffolds					1434:1442	Our 3D printed scaffolds	1419:1442	Our 3D printed scaffolds	1419:1442	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	12	69	theme	constructs	1886:1895	arg1	fabrication					1864:1874	the fabrication	1860:1874	the fabrication of tissue constructs for transplantation and other biomedical applications	1860:1949	Three-dimensional (3D) bioprinting has become a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications.
35288311	11	70	theme	life	1790:1793	arg1	quality					1779:1785	quality	1779:1785	quality of life	1779:1793	STATEMENT OF SIGNIFICANCE: Hair loss remains a challenging clinical problem that influences quality of life.
35288311	15	71	theme	entire	2466:2471	arg1	HFs					2473:2475	entire HFs	2466:2475	entire HFs	2466:2475	The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss.
35288311	9	72	theme	suitable	1454:1461	arg1	microenvironment					1463:1478	a suitable microenvironment	1452:1478	a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss	1452:1593	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	2	73	theme	3D	296:297	arg1	technique					311:319	a 3D bioprinting technique	294:319	a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo	294:450	Here, we demonstrate a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo.
35288311	14	74	theme	DPCs	2287:2290	arg1	spheroids					2274:2282	self-aggregating spheroids	2257:2282	self-aggregating spheroids of DPCs	2257:2290	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	0	75	theme	follicle	73:80	arg1	regeneration					82:93	tissue-engineered hair follicle regeneration	50:93	tissue-engineered hair follicle regeneration	50:93	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration.
35288311	13	76	theme	composite	2066:2074	arg1	scaffold					2076:2083	a multi-layer composite scaffold	2052:2083	a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body	2052:2198	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	14	77	theme	spheroids	2274:2282	arg1	formation					2244:2252	the controllable formation	2227:2252	the controllable formation of self-aggregating spheroids of DPCs	2227:2290	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	4	78	with	scaffold	749:756	arg1	epidermis-					763:772	epidermis-	763:772	epidermis-	763:772	The bioprinted scaffold with epidermis- and dermis-like structure was subsequently transplanted into full-thickness wounds in nude mice.
35288311	4	78	with	scaffold	749:756	arg1	structure					790:798	dermis-like structure	778:798	dermis-like structure	778:798	The bioprinted scaffold with epidermis- and dermis-like structure was subsequently transplanted into full-thickness wounds in nude mice.
35288311	7	79	theme	epidermal	1171:1179	arg1	cells					1181:1185	The dermal and epidermal cells	1156:1185	The dermal and epidermal cells	1156:1185	The dermal and epidermal cells self-assembled successfully into immature HFs in vitro.
35288311	13	80	theme	gelatin/alginate	2013:2028	arg1	hydrogel					2030:2037	a gelatin/alginate hydrogel	2011:2037	a gelatin/alginate hydrogel	2011:2037	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	7	81	theme	dermal	1160:1165	arg1	cells					1181:1185	The dermal and epidermal cells	1156:1185	The dermal and epidermal cells	1156:1185	The dermal and epidermal cells self-assembled successfully into immature HFs in vitro.
35288311	12	82	theme	tissue	1879:1884	arg1	constructs					1886:1895	tissue constructs	1879:1895	tissue constructs	1879:1895	Three-dimensional (3D) bioprinting has become a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications.
35288311	9	83	theme	entire	1503:1508	arg1	HFs					1510:1512	entire HFs	1503:1512	entire HFs	1503:1512	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	9	84	dep	microenvironment	1463:1478	arg1	make					1524:1527	make	1524:1527	could make a significant contribution in the medical management of hair loss	1518:1593	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	9	84	dep	microenvironment	1463:1478	arg1	regenerate					1492:1501	regenerate	1492:1501	to regenerate entire HFs	1489:1512	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	14	85	theme	controllable	2231:2242	arg1	formation					2244:2252	the controllable formation	2227:2252	the controllable formation of self-aggregating spheroids of DPCs	2227:2290	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	12	86	theme	useful	1844:1849	arg1	tool					1851:1854	a useful tool	1842:1854	a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications	1842:1949	Three-dimensional (3D) bioprinting has become a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications.
35288311	6	87	theme	spheroids	1063:1071	arg1	formation					1029:1037	the formation	1025:1037	the formation of self-aggregating DPC spheroids	1025:1071	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	5	88	theme	suitable	908:915	arg1	cytocompatibility					917:933	suitable cytocompatibility	908:933	suitable cytocompatibility	908:933	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	6	89	theme	self-aggregating	1042:1057	arg1	spheroids					1063:1071	self-aggregating DPC spheroids	1042:1071	self-aggregating DPC spheroids	1042:1071	It also facilitated the formation of self-aggregating DPC spheroids and restored DPC genes associated with hair induction (ALP, β-catenin, and α-SMA).
35288311	14	90	theme	new	2206:2208	arg1	approach					2210:2217	This new approach	2201:2217	This new approach	2201:2217	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	8	91	theme	dot	1394:1396	arg1	bioprinting					1398:1408	the dot bioprinting	1390:1408	the dot bioprinting of DPCs	1390:1416	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	9	92	theme	significant	1531:1541	arg1	contribution					1543:1554	a significant contribution	1529:1554	a significant contribution in the medical management of hair loss	1529:1593	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	2	93	theme	HF	424:425	arg1	microenvironment					427:442	the HF microenvironment	420:442	the HF microenvironment	420:442	Here, we demonstrate a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo.
35288311	0	94	theme	hydrogel	37:44	arg1	bioprinting					3:13	3D bioprinting	0:13	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration	0:93	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration.
35288311	13	95	theme	human	2189:2193	arg1	body					2195:2198	the human body	2185:2198	the human body	2185:2198	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	13	96	used	used	1970:1973	arg2	we					1967:1968	we	1967:1968	we	1967:1968	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	8	97	theme	grid	1355:1358	arg1	structure					1360:1368	the hierarchical grid structure	1338:1368	the hierarchical grid structure of the scaffold	1338:1384	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	2	98	theme	composite	390:398	arg1	scaffold					400:407	a multilayer composite scaffold	377:407	a multilayer composite scaffold simulating the HF microenvironment	377:442	Here, we demonstrate a 3D bioprinting technique based on a gelatin/alginate hydrogel (GAH) to construct a multilayer composite scaffold simulating the HF microenvironment in vivo.
35288311	0	99	theme	tissue-engineered	50:66	arg1	regeneration					82:93	tissue-engineered hair follicle regeneration	50:93	tissue-engineered hair follicle regeneration	50:93	3D bioprinting of a gelatin-alginate hydrogel for tissue-engineered hair follicle regeneration.
35288311	13	100	theme	cells	2169:2173	arg1	microenvironment					2134:2149	the microenvironment	2130:2149	the microenvironment of dermal papilla cells (DPCs) in the human body	2130:2198	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	12	101	theme	Three-dimensional	1796:1812	arg1	bioprinting					1819:1829	Three-dimensional (3D) bioprinting	1796:1829	Three-dimensional (3D) bioprinting	1796:1829	Three-dimensional (3D) bioprinting has become a useful tool for the fabrication of tissue constructs for transplantation and other biomedical applications.
35288311	9	102	theme	medical	1563:1569	arg1	management					1571:1580	the medical management	1559:1580	the medical management of hair loss	1559:1593	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	3	103	theme	umbilical	478:486	arg1	HUVECs					512:517	HUVECs	512:517	HUVECs	512:517	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	3	103	theme	umbilical	478:486	arg1	cells					505:509	human umbilical vein endothelial cells	472:509	human umbilical vein endothelial cells (HUVECs)	472:518	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	5	104	theme	DPCs	978:981	arg1	ability					967:973	increased the proliferation ability	939:973	increased the proliferation ability of DPCs (1.2-fold; P < 0.05)	939:1002	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	5	104	theme	DPCs	978:981	arg1	cytocompatibility					917:933	suitable cytocompatibility	908:933	suitable cytocompatibility	908:933	The multilayer scaffold demonstrated suitable cytocompatibility and increased the proliferation ability of DPCs (1.2-fold; P < 0.05).
35288311	13	105	from	microenvironment	2134:2149	arg1	body					2195:2198	the human body	2185:2198	the human body	2185:2198	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	8	106	theme	scaffold	1377:1384	arg1	bioprinting					1398:1408	the dot bioprinting	1390:1408	the dot bioprinting of DPCs	1390:1416	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	8	106	theme	scaffold	1377:1384	arg1	structure					1360:1368	the hierarchical grid structure	1338:1368	the hierarchical grid structure of the scaffold	1338:1384	HFs were regenerated in the appropriate orientation in vivo, which can mainly be attributed to the hierarchical grid structure of the scaffold and the dot bioprinting of DPCs.
35288311	10	107	theme	skin	1646:1649	arg1	tissue					1651:1656	skin tissue	1646:1656	skin tissue	1646:1656	This method may also have broader applications in skin tissue (and appendage) engineering.
35288311	15	108	theme	loss	2544:2547	arg1	management					2525:2534	the medical management	2513:2534	the medical management of hair loss	2513:2547	The ability to regenerate entire HFs should have a significant impact on the medical management of hair loss.
35288311	9	109	theme	loss	1590:1593	arg1	management					1571:1580	the medical management	1559:1580	the medical management of hair loss	1559:1593	Our 3D printed scaffolds provide a suitable microenvironment for DPCs to regenerate entire HFs and could make a significant contribution in the medical management of hair loss.
35288311	3	110	theme	gelatin	630:636	arg1	mixture					619:625	a mixture	617:625	a mixture of gelatin and alginate	617:649	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35288311	13	111	theme	dermal	2154:2159	arg1	DPCs					2176:2179	DPCs	2176:2179	DPCs	2176:2179	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	13	111	theme	dermal	2154:2159	arg1	cells					2169:2173	dermal papilla cells	2154:2173	dermal papilla cells (DPCs)	2154:2180	In this study, we used a 3D bioprinting technique based on a gelatin/alginate hydrogel to construct a multi-layer composite scaffold with cuticular and corium layers to simulate the microenvironment of dermal papilla cells (DPCs) in the human body.
35288311	14	112	theme	interactions	2387:2398	arg1	matrix					2336:2341	a physiologically relevant extracellular matrix	2295:2341	a physiologically relevant extracellular matrix	2295:2341	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	14	112	theme	interactions	2387:2398	arg1	initiation					2351:2360	the initiation	2347:2360	the initiation of epidermal-mesenchymal interactions	2347:2398	This new approach permits the controllable formation of self-aggregating spheroids of DPCs in a physiologically relevant extracellular matrix and the initiation of epidermal-mesenchymal interactions, which results in HF formation in vivo.
35288311	3	113	theme	composite	714:722	arg1	scaffold					724:731	a composite scaffold	712:731	a composite scaffold	712:731	Fibroblasts (FBs), human umbilical vein endothelial cells (HUVECs), dermal papilla cells (DPCs), and epidermal cells (EPCs) were encapsulated in GAH (prepared from a mixture of gelatin and alginate) and respectively 3D-bioprinted into the different layers of a composite scaffold.
35572712	0	0	theme	Proteome	82:89	arg1	Microbiome					30:39	Human Gut Microbiome	20:39	Human Gut Microbiome	20:39	Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome.
35572712	0	0	theme	Proteome	82:89	arg1	Composition					54:64	N-Glycan Composition	45:64	N-Glycan Composition	45:64	Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome.
35572712	1	1	gly	glycosylation	150:162	arg1	proteins					167:174	proteins	167:174	proteins	167:174	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	3	2	theme	microbiome	695:704	arg1	diversity					706:714	alpha and beta microbiome diversity	680:714	alpha and beta microbiome diversity	680:714	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	1	3	theme	human	138:142	arg1	body					144:147	the human body	134:147	the human body	134:147	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	5	4	theme	well-selected	1188:1200	arg1	groups					1210:1215	well-selected patient groups	1188:1215	well-selected patient groups	1188:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	0	5	theme	Plasma	75:80	arg1	Proteome					82:89	Total Plasma Proteome	69:89	Total Plasma Proteome	69:89	Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome.
35572712	4	6	theme	significant	836:846	arg1	associations					848:859	significant associations	836:859	significant associations with specific glycans	836:881	Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown.
35572712	5	7	theme	patient	1202:1208	arg1	groups					1210:1215	well-selected patient groups	1188:1215	well-selected patient groups	1188:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	5	8	theme	Further	1120:1126	arg1	studies					1128:1134	Further studies	1120:1134	Further studies of glycans linked to microbiome-related proteins in well-selected patient groups	1120:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	4	9	theme	specific	866:873	arg1	glycans					875:881	specific glycans	866:881	specific glycans	866:881	Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown.
35572712	1	10	theme	immune	243:248	arg1	system					250:255	the immune system	239:255	the immune system	239:255	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	5	11	theme	immune	1068:1073	arg1	system					1075:1080	immune system	1068:1080	the immune system proteins	1064:1089	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	2	12	theme	G	574:574	arg1	N-glycome					546:554	N-glycome	546:554	N-glycome of immunoglobulin G	546:574	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	1	13	with	interaction	275:285	arg1	consortium					312:321	the human microbial consortium	292:321	the human microbial consortium	292:321	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	5	14	theme	system	1075:1080	arg1	proteins					1082:1089	the immune system proteins	1064:1089	the immune system proteins	1064:1089	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	5	14	theme	system	1075:1080	arg1	immunoglobulin					1102:1115	immunoglobulin	1102:1115	immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations	1102:1276	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	4	15	theme	Clostridium	814:824	arg1	innocuum					826:833	Clostridium innocuum	814:833	Clostridium innocuum	814:833	Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown.
35572712	3	16	from	linkages	598:605	arg1	level					627:631	the multivariate level	610:631	the multivariate level	610:631	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	2	17	theme	gut	489:491	arg1	microbiome					493:502	the gut microbiome	485:502	the gut microbiome	485:502	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	2	18	theme	potential	457:465	arg1	N-glycome					546:554	N-glycome	546:554	N-glycome of immunoglobulin G	546:574	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	2	18	theme	potential	457:465	arg1	relations					467:475	potential relations	457:475	potential relations	457:475	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	1	19	theme	gut	353:355	arg1	microbiome					357:366	the gut microbiome	349:366	the gut microbiome	349:366	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	1	20	from	entities	122:129	arg1	body					144:147	the human body	134:147	the human body	134:147	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	3	21	theme	plasma	730:735	arg1	profile					747:753	the blood plasma N-glycome profile	720:753	the blood plasma N-glycome profile	720:753	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	5	22	from	proteins	1176:1183	arg1	groups					1210:1215	well-selected patient groups	1188:1215	well-selected patient groups	1188:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	1	23	theme	proteins	167:174	arg1	glycosylation					150:162	glycosylation	150:162	glycosylation of proteins	150:174	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	0	24	theme	Gut	26:28	arg1	Microbiome					30:39	Human Gut Microbiome	20:39	Human Gut Microbiome	20:39	Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome.
35572712	3	25	theme	N-glycome	737:745	arg1	profile					747:753	the blood plasma N-glycome profile	720:753	the blood plasma N-glycome profile	720:753	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	5	26	theme	total	974:978	arg1	N-glycome					987:995	the total plasma N-glycome	970:995	the total plasma N-glycome	970:995	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	5	27	attach	linked	1147:1152	arg1	proteins					1176:1183	microbiome-related proteins	1157:1183	microbiome-related proteins in well-selected patient groups	1157:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	5	27	attach	linked	1147:1152	arg2	glycans					1139:1145	glycans	1139:1145	glycans linked to microbiome-related proteins in well-selected patient groups	1139:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	0	28	theme	Human	20:24	arg1	Microbiome					30:39	Human Gut Microbiome	20:39	Human Gut Microbiome	20:39	Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome.
35572712	5	29	theme	non-trivial	930:940	arg1	link					957:960	a non-trivial, possibly weak link	928:960	a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations	928:1276	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	2	30	theme	healthy	389:395	arg1	individuals					397:407	194 healthy individuals	385:407	194 healthy individuals	385:407	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	3	31	theme	blood	724:728	arg1	profile					747:753	the blood plasma N-glycome profile	720:753	the blood plasma N-glycome profile	720:753	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	2	32	theme	plasma	518:523	arg1	N-glycome					525:533	the blood plasma N-glycome	508:533	the blood plasma N-glycome	508:533	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	5	33	theme	related	1053:1059	arg1	glycans					1045:1051	glycans	1045:1051	glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations	1045:1276	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	5	34	theme	plasma	980:985	arg1	N-glycome					987:995	the total plasma N-glycome	970:995	the total plasma N-glycome	970:995	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	2	35	theme	blood	512:516	arg1	N-glycome					525:533	the blood plasma N-glycome	508:533	the blood plasma N-glycome	508:533	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	3	36	theme	multivariate	614:625	arg1	level					627:631	the multivariate level	610:631	the multivariate level	610:631	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	2	37	theme	immunoglobulin	559:572	arg1	G					574:574	immunoglobulin G	559:574	immunoglobulin G	559:574	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	5	38	theme	microbiome-related	1157:1174	arg1	proteins					1176:1183	microbiome-related proteins	1157:1183	microbiome-related proteins in well-selected patient groups	1157:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	3	39	theme	beta	690:693	arg1	diversity					706:714	alpha and beta microbiome diversity	680:714	alpha and beta microbiome diversity	680:714	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	0	40	theme	N-Glycan	45:52	arg1	Composition					54:64	N-Glycan Composition	45:64	N-Glycan Composition	45:64	Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome.
35572712	5	41	theme	glycans	1139:1145	arg1	studies					1128:1134	Further studies	1120:1134	Further studies of glycans linked to microbiome-related proteins in well-selected patient groups	1120:1215	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	3	42	theme	alpha	680:684	arg1	diversity					706:714	alpha and beta microbiome diversity	680:714	alpha and beta microbiome diversity	680:714	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	2	43	theme	associational	426:438	arg1	study					440:444	an associational study	423:444	an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G	423:574	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	5	44	theme	gut	1005:1007	arg1	microbiome					1009:1018	the gut microbiome	1001:1018	the gut microbiome	1001:1018	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	1	45	theme	human	296:300	arg1	consortium					312:321	the human microbial consortium	292:321	the human microbial consortium	292:321	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	1	46	theme	dynamic	114:120	arg1	entities					122:129	the most dynamic entities	105:129	the most dynamic entities in the human body	105:147	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	1	47	theme	entities	122:129	arg1	entities					122:129	the most dynamic entities	105:129	the most dynamic entities in the human body	105:147	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	1	47	theme	entities	122:129	arg1	one					98:100	one	98:100	one	98:100	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	4	48	with	associations	848:859	arg1	glycans					875:881	specific glycans	866:881	specific glycans	866:881	Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown.
35572712	1	49	theme	organismal	207:216	arg1	machinery					218:226	the organismal machinery	203:226	the organismal machinery	203:226	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	0	50	theme	Total	69:73	arg1	Proteome					82:89	Total Plasma Proteome	69:89	Total Plasma Proteome	69:89	Association Between Human Gut Microbiome and N-Glycan Composition of Total Plasma Proteome.
35572712	3	51	theme	strong	591:596	arg1	linkages					598:605	strong linkages	591:605	strong linkages on the multivariate level	591:631	While lacking strong linkages on the multivariate level, we were able to identify associations between alpha and beta microbiome diversity and the blood plasma N-glycome profile.
35572712	5	52	dep	immunoglobulin	1102:1115	arg1	required					1221:1228	required	1221:1228	are required to conclusively establish specific associations	1217:1276	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	1	53	theme	machinery	218:226	arg1	activity					191:198	the activity	187:198	the activity of the organismal machinery, including the immune system	187:255	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	1	54	theme	microbial	302:310	arg1	consortium					312:321	the human microbial consortium	292:321	the human microbial consortium	292:321	Being one of the most dynamic entities in the human body, glycosylation of proteins fine-tunes the activity of the organismal machinery, including the immune system, and mediates the interaction with the human microbial consortium, typically represented by the gut microbiome.
35572712	4	55	theme	bacterial	774:782	arg1	genera					784:789	two bacterial genera	770:789	two bacterial genera	770:789	Moreover, for two bacterial genera, namely, Bilophila and Clostridium innocuum, significant associations with specific glycans were also shown.
35572712	2	56	from	individuals	397:407	arg1	data					375:378	data	375:378	data from 194 healthy individuals	375:407	Using data from 194 healthy individuals, we conducted an associational study to uncover potential relations between the gut microbiome and the blood plasma N-glycome, including N-glycome of immunoglobulin G.
35572712	5	57	dep	non-trivial	930:940	arg1	weak					952:955	weak	952:955	weak	952:955	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
35572712	5	58	theme	specific	1256:1263	arg1	associations					1265:1276	specific associations	1256:1276	specific associations	1256:1276	The study's results suggest a non-trivial, possibly weak link between the total plasma N-glycome and the gut microbiome, predominantly involving glycans related to the immune system proteins, including immunoglobulin G. Further studies of glycans linked to microbiome-related proteins in well-selected patient groups are required to conclusively establish specific associations.
36740600	1	0	theme	dentistry	337:345	arg1	application					347:357	regenerative dentistry application	324:357	regenerative dentistry application	324:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	18	1	theme	favorable	2258:2266	arg1	properties					2247:2256	mechanical and biological properties	2221:2256	mechanical and biological properties favorable for the regeneration process	2221:2295	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	18	1	theme	favorable	2258:2266	arg1	characteristics					2204:2218	morphological characteristics	2190:2218	morphological characteristics	2190:2218	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	11	2	dep	the	1475:1477	arg1	GO					1479:1480	GO	1479:1480	GO	1479:1480	Raman spectroscopy analysis evidenced a high quality of the GO.
36740600	13	3	theme	adhered	1659:1665	arg1	HA					1639:1640	HA	1639:1640	HA incorporated and adhered to the polymer	1639:1680	SEM analysis showed pores in the scaffolds, in addition to HA incorporated and adhered to the polymer.
36740600	11	4	theme	spectroscopy	1425:1436	arg1	analysis					1438:1445	Raman spectroscopy analysis	1419:1445	Raman spectroscopy analysis	1419:1445	Raman spectroscopy analysis evidenced a high quality of the GO.
36740600	2	5	theme	gum	381:383	arg1	complex					390:396	The chitosan-xanthan gum (CX) complex	360:396	The chitosan-xanthan gum (CX) complex	360:396	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	8	6	theme	contact	1151:1157	arg1	data					1165:1168	contact angle data	1151:1168	contact angle data	1151:1168	Compressive strength and contact angle data were statistically analyzed by one-way ANOVA, followed by Tukey's test (α = 0.05).
36740600	18	7	theme	biological	2236:2245	arg1	properties					2247:2256	mechanical and biological properties	2221:2256	mechanical and biological properties favorable for the regeneration process	2221:2295	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	18	7	theme	biological	2236:2245	arg1	characteristics					2204:2218	morphological characteristics	2190:2218	morphological characteristics	2190:2218	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	3	8	theme	electron	735:742	arg1	microscopy					744:753	scanning electron microscopy	726:753	scanning electron microscopy (SEM)	726:759	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	3	8	theme	electron	735:742	arg1	SEM					756:758	SEM	756:758	SEM	756:758	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	14	9	theme	highest	1781:1787	arg1	angle					1797:1801	the highest contact angle	1777:1801	the highest contact angle	1777:1801	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	3	10	theme	contact	765:771	arg1	angle					773:777	contact angle	765:777	contact angle	765:777	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	11	11	theme	high	1459:1462	arg1	quality					1464:1470	a high quality	1457:1470	a high quality of the GO	1457:1480	Raman spectroscopy analysis evidenced a high quality of the GO.
36740600	2	12	dep	Oxides	494:499	arg1	GO					502:503	GO	502:503	GO	502:503	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	1	13	theme	Chitosan/Xanthan/Hydroxyapatite-Graphene	220:259	arg1	nanocomposite					267:279	Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite	220:279	Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application	220:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	13	14	theme	incorporated	1642:1653	arg1	HA					1639:1640	HA	1639:1640	HA incorporated and adhered to the polymer	1639:1680	SEM analysis showed pores in the scaffolds, in addition to HA incorporated and adhered to the polymer.
36740600	7	15	theme	two-way	1080:1086	arg1	ANOVA					1074:1078	ANOVA two-way	1074:1086	ANOVA two-way	1074:1086	In vitro Indirect Cytotoxicity assay data was statistically analyzed by ANOVA two-way, followed by Tukey's test (α = 0.05).
36740600	1	16	theme	nanocomposite	267:279	arg1	scaffolds					207:215	polymeric scaffolds	197:215	polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application	197:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	16	17	theme	scaffold	1994:2001	arg1	surface					2003:2009	the scaffold surface	1990:2009	the scaffold surface	1990:2009	In the bioactivity test, the apatite crystals precipitation on the scaffold surface was observed.
36740600	7	18	theme	Indirect	1011:1018	arg1	assay					1033:1037	In vitro Indirect Cytotoxicity assay data	1002:1042	In vitro Indirect Cytotoxicity assay data	1002:1042	In vitro Indirect Cytotoxicity assay data was statistically analyzed by ANOVA two-way, followed by Tukey's test (α = 0.05).
36740600	14	19	theme	Contact	1683:1689	arg1	test					1697:1700	Contact angle test	1683:1700	Contact angle test	1683:1700	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	7	20	theme	In	1002:1003	arg1	assay					1033:1037	In vitro Indirect Cytotoxicity assay data	1002:1042	In vitro Indirect Cytotoxicity assay data	1002:1042	In vitro Indirect Cytotoxicity assay data was statistically analyzed by ANOVA two-way, followed by Tukey's test (α = 0.05).
36740600	14	21	theme	hydrophilic	1731:1741	arg1	characteristic					1743:1756	a hydrophilic characteristic	1729:1756	a hydrophilic characteristic	1729:1756	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	18	22	theme	mechanical	2221:2230	arg1	properties					2247:2256	mechanical and biological properties	2221:2256	mechanical and biological properties favorable for the regeneration process	2221:2295	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	18	22	theme	mechanical	2221:2230	arg1	characteristics					2204:2218	morphological characteristics	2190:2218	morphological characteristics	2190:2218	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	14	23	theme	contact	1789:1795	arg1	angle					1797:1801	the highest contact angle	1777:1801	the highest contact angle	1777:1801	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	9	24	dep	%	1340:1340	arg1	CXHA					1324:1327	CXHA	1324:1327	CXHA	1324:1327	XRD showed the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%.
36740600	15	25	dep	%	1841:1841	arg1	GO					1843:1844	GO	1843:1844	GO	1843:1844	1.0 wt% GO significantly increased the compressive strength compared to other compositions.
36740600	5	26	theme	in	849:850	arg1	test					900:903	the in vitro cytotoxicity test	874:903	the in vitro cytotoxicity test (MTT test)	874:914	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	26	theme	in	849:850	arg1	bioactivity					858:868	The in vitro bioactivity	845:868	The in vitro bioactivity	845:868	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	26	theme	in	849:850	arg1	well					933:936	well	933:936	well	933:936	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	27	theme	MTT	906:908	arg1	test					910:913	MTT test	906:913	MTT test	906:913	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	27	theme	MTT	906:908	arg1	test					900:903	the in vitro cytotoxicity test	874:903	the in vitro cytotoxicity test (MTT test)	874:914	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	14	28	contain	have	1724:1727	arg2	characteristic					1743:1756	a hydrophilic characteristic	1729:1756	a hydrophilic characteristic	1729:1756	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	14	28	contain	have	1724:1727	arg1	scaffolds					1714:1722	scaffolds	1714:1722	scaffolds	1714:1722	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	18	29	contain	have	2185:2188	arg1	they					2180:2183	they	2180:2183	they	2180:2183	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	18	29	contain	have	2185:2188	arg2	characteristics					2204:2218	morphological characteristics	2190:2218	morphological characteristics	2190:2218	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	18	29	contain	have	2185:2188	arg2	properties					2247:2256	mechanical and biological properties	2221:2256	mechanical and biological properties favorable for the regeneration process	2221:2295	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	11	30	theme	Raman	1419:1423	arg1	analysis					1438:1445	Raman spectroscopy analysis	1419:1445	Raman spectroscopy analysis	1419:1445	Raman spectroscopy analysis evidenced a high quality of the GO.
36740600	0	31	theme	dentin-pulp	106:116	arg1	regeneration					126:137	dentin-pulp complex regeneration	106:137	dentin-pulp complex regeneration	106:137	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold associated with mesenchymal stem cells for dentin-pulp complex regeneration.
36740600	16	32	from	precipitation	1973:1985	arg1	surface					2003:2009	the scaffold surface	1990:2009	the scaffold surface	1990:2009	In the bioactivity test, the apatite crystals precipitation on the scaffold surface was observed.
36740600	16	33	theme	bioactivity	1934:1944	arg1	test					1946:1949	the bioactivity test	1930:1949	the bioactivity test	1930:1949	In the bioactivity test, the apatite crystals precipitation on the scaffold surface was observed.
36740600	9	34	from	peaks	1300:1304	arg1	structures					1313:1322	the structures	1309:1322	the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%	1309:1354	XRD showed the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%.
36740600	10	35	theme	amino	1370:1374	arg1	bands					1391:1395	amino and carboxylic bands	1370:1395	amino and carboxylic bands characteristic of CX	1370:1416	FT-IR showed amino and carboxylic bands characteristic of CX.
36740600	2	36	theme	Oxides	494:499	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	36	theme	Oxides	494:499	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	37	theme	%	527:527	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	37	theme	%	527:527	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	16	38	theme	apatite	1956:1962	arg1	crystals					1964:1971	the apatite crystals	1952:1971	the apatite crystals precipitation on the scaffold surface	1952:2009	In the bioactivity test, the apatite crystals precipitation on the scaffold surface was observed.
36740600	17	39	theme	high	2041:2044	arg1	viability					2051:2059	high cell viability	2041:2059	high cell viability	2041:2059	MTT test showed high cell viability in CXHAGO 1.0% and CXHAGO 1.5% scaffold.
36740600	2	40	theme	different	475:483	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	40	theme	different	475:483	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	0	41	theme	Chitosan/Xanthan/Hydroxyapatite-graphene	0:39	arg1	scaffold					54:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold	0:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold	0:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold associated with mesenchymal stem cells for dentin-pulp complex regeneration.
36740600	14	42	theme	angle	1691:1695	arg1	test					1697:1700	Contact angle test	1683:1700	Contact angle test	1683:1700	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	12	43	theme	mass	1514:1517	arg1	loss					1519:1522	the mass loss	1510:1522	the mass loss associated with the CX degradation by depolymerization	1510:1577	In the TGA it was observed the mass loss associated with the CX degradation by depolymerization.
36740600	10	44	theme	characteristic	1397:1410	arg1	bands					1391:1395	amino and carboxylic bands	1370:1395	amino and carboxylic bands characteristic of CX	1370:1416	FT-IR showed amino and carboxylic bands characteristic of CX.
36740600	0	45	theme	porous	47:52	arg1	scaffold					54:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold	0:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold	0:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold associated with mesenchymal stem cells for dentin-pulp complex regeneration.
36740600	18	46	theme	regenerative	2137:2148	arg1	application					2160:2170	regenerative dentistry application	2137:2170	regenerative dentistry application	2137:2170	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	2	47	theme	%	536:536	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	47	theme	%	536:536	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	10	48	theme	carboxylic	1380:1389	arg1	bands					1391:1395	amino and carboxylic bands	1370:1395	amino and carboxylic bands characteristic of CX	1370:1416	FT-IR showed amino and carboxylic bands characteristic of CX.
36740600	1	49	theme	polymeric	197:205	arg1	scaffolds					207:215	polymeric scaffolds	197:215	polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application	197:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	13	50	from	pores	1600:1604	arg1	scaffolds					1613:1621	the scaffolds	1609:1621	the scaffolds	1609:1621	SEM analysis showed pores in the scaffolds, in addition to HA incorporated and adhered to the polymer.
36740600	14	51	theme	p	1824:1824	arg1	lowest					1816:1821	lowest	1816:1821	lowest	1816:1821	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	14	51	theme	p	1824:1824	arg1	<					1826:1826	p < 0.05	1824:1831	p < 0.05	1824:1831	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	5	52	dep	in	878:879	arg1	vitro					881:885	vitro	881:885	vitro	881:885	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	2	53	theme	Oxide	442:446	arg1	nanocomposite					456:468	Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite	418:468	Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	418:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	0	54	theme	mesenchymal	79:89	arg1	cells					96:100	mesenchymal stem cells	79:100	mesenchymal stem cells for dentin-pulp complex regeneration	79:137	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold associated with mesenchymal stem cells for dentin-pulp complex regeneration.
36740600	10	55	theme	CX	1415:1416	arg1	characteristic					1397:1410	characteristic	1397:1410	characteristic	1397:1410	FT-IR showed amino and carboxylic bands characteristic of CX.
36740600	1	56	theme	mesenchymal	297:307	arg1	cells					314:318	mesenchymal stem cells	297:318	mesenchymal stem cells for regenerative dentistry application	297:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	2	57	with	nanocomposite	456:468	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	57	with	nanocomposite	456:468	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	7	58	dep	analyzed	1062:1069	arg1	followed					1089:1096	followed	1089:1096	followed by Tukey's test (α = 0.05)	1089:1123	In vitro Indirect Cytotoxicity assay data was statistically analyzed by ANOVA two-way, followed by Tukey's test (α = 0.05).
36740600	3	59	theme	thermogravimetric	692:708	arg1	TGA					720:722	TGA	720:722	TGA	720:722	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	3	59	theme	thermogravimetric	692:708	arg1	analysis					710:717	thermogravimetric analysis	692:717	thermogravimetric analysis (TGA)	692:723	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	4	60	theme	compressive	823:833	arg1	strength					835:842	compressive strength	823:842	compressive strength	823:842	The mechanical properties were assessed by compressive strength.
36740600	3	61	theme	scanning	726:733	arg1	microscopy					744:753	scanning electron microscopy	726:753	scanning electron microscopy (SEM)	726:759	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	3	61	theme	scanning	726:733	arg1	SEM					756:758	SEM	756:758	SEM	756:758	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	1	62	theme	regenerative	324:335	arg1	application					347:357	regenerative dentistry application	324:357	regenerative dentistry application	324:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	5	63	dep	in	849:850	arg1	vitro					852:856	vitro	852:856	vitro	852:856	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	7	64	dep	assay	1033:1037	arg1	data					1039:1042	data	1039:1042	In vitro Indirect Cytotoxicity assay data	1002:1042	In vitro Indirect Cytotoxicity assay data was statistically analyzed by ANOVA two-way, followed by Tukey's test (α = 0.05).
36740600	8	65	theme	Compressive	1126:1136	arg1	strength					1138:1145	Compressive strength	1126:1145	Compressive strength	1126:1145	Compressive strength and contact angle data were statistically analyzed by one-way ANOVA, followed by Tukey's test (α = 0.05).
36740600	2	66	theme	chitosan-xanthan	364:379	arg1	complex					390:396	The chitosan-xanthan gum (CX) complex	360:396	The chitosan-xanthan gum (CX) complex	360:396	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	5	67	theme	cytotoxicity	887:898	arg1	test					910:913	MTT test	906:913	MTT test	906:913	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	67	theme	cytotoxicity	887:898	arg1	well					933:936	well	933:936	well	933:936	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	67	theme	cytotoxicity	887:898	arg1	bioactivity					858:868	The in vitro bioactivity	845:868	The in vitro bioactivity	845:868	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	67	theme	cytotoxicity	887:898	arg1	test					900:903	the in vitro cytotoxicity test	874:903	the in vitro cytotoxicity test (MTT test)	874:914	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	68	theme	in	878:879	arg1	test					910:913	MTT test	906:913	MTT test	906:913	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	68	theme	in	878:879	arg1	well					933:936	well	933:936	well	933:936	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	68	theme	in	878:879	arg1	bioactivity					858:868	The in vitro bioactivity	845:868	The in vitro bioactivity	845:868	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	5	68	theme	in	878:879	arg1	test					900:903	the in vitro cytotoxicity test	874:903	the in vitro cytotoxicity test (MTT test)	874:914	The in vitro bioactivity and the in vitro cytotoxicity test (MTT test) were analyzed as well.
36740600	16	69	dep	crystals	1964:1971	arg1	precipitation					1973:1985	precipitation	1973:1985	precipitation	1973:1985	In the bioactivity test, the apatite crystals precipitation on the scaffold surface was observed.
36740600	8	70	theme	angle	1159:1163	arg1	data					1165:1168	contact angle data	1151:1168	contact angle data	1151:1168	Compressive strength and contact angle data were statistically analyzed by one-way ANOVA, followed by Tukey's test (α = 0.05).
36740600	11	71	theme	the	1475:1477	arg1	quality					1464:1470	a high quality	1457:1470	a high quality of the GO	1457:1480	Raman spectroscopy analysis evidenced a high quality of the GO.
36740600	3	72	dep	Fourier	624:630	arg1	Transform					632:640	Transform	632:640	Transform Infrared Spectroscopy (FTIR)	632:669	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	9	73	from	presence	1268:1275	arg1	structures					1313:1322	the structures	1309:1322	the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%	1309:1354	XRD showed the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%.
36740600	17	74	dep	%	2074:2074	arg1	1.0					2071:2073	1.0	2071:2073	1.0	2071:2073	MTT test showed high cell viability in CXHAGO 1.0% and CXHAGO 1.5% scaffold.
36740600	18	75	theme	regeneration	2276:2287	arg1	process					2289:2295	the regeneration process	2272:2295	the regeneration process	2272:2295	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	16	76	located	observed	2015:2022	arg2	crystals					1964:1971	the apatite crystals	1952:1971	the apatite crystals precipitation on the scaffold surface	1952:2009	In the bioactivity test, the apatite crystals precipitation on the scaffold surface was observed.
36740600	16	76	located	observed	2015:2022	arg1	test					1946:1949	the bioactivity test	1930:1949	the bioactivity test	1930:1949	In the bioactivity test, the apatite crystals precipitation on the scaffold surface was observed.
36740600	3	77	theme	X-ray	599:603	arg1	diffraction					605:615	X-ray diffraction	599:615	X-ray diffraction (XRD)	599:621	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	3	77	theme	X-ray	599:603	arg1	XRD					618:620	XRD	618:620	XRD	618:620	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	1	78	theme	Oxide	261:265	arg1	nanocomposite					267:279	Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite	220:279	Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application	220:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	15	79	theme	wt	1839:1840	arg1	%					1841:1841	1.0 wt% GO	1835:1844	1.0 wt% GO	1835:1844	1.0 wt% GO significantly increased the compressive strength compared to other compositions.
36740600	17	80	dep	%	2090:2090	arg1	1.5					2087:2089	1.5	2087:2089	1.5	2087:2089	MTT test showed high cell viability in CXHAGO 1.0% and CXHAGO 1.5% scaffold.
36740600	7	81	theme	Cytotoxicity	1020:1031	arg1	assay					1033:1037	In vitro Indirect Cytotoxicity assay data	1002:1042	In vitro Indirect Cytotoxicity assay data	1002:1042	In vitro Indirect Cytotoxicity assay data was statistically analyzed by ANOVA two-way, followed by Tukey's test (α = 0.05).
36740600	8	82	theme	one-way	1201:1207	arg1	ANOVA					1209:1213	one-way ANOVA	1201:1213	one-way ANOVA	1201:1213	Compressive strength and contact angle data were statistically analyzed by one-way ANOVA, followed by Tukey's test (α = 0.05).
36740600	12	83	located	observed	1501:1508	arg2	it					1494:1495	it	1494:1495	it	1494:1495	In the TGA it was observed the mass loss associated with the CX degradation by depolymerization.
36740600	12	83	located	observed	1501:1508	arg1	TGA					1490:1492	the TGA	1486:1492	the TGA	1486:1492	In the TGA it was observed the mass loss associated with the CX degradation by depolymerization.
36740600	1	84	theme	paper	156:160	arg1	aim					144:146	The aim	140:146	The aim of this paper	140:160	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	6	85	dep	Normality	969:977	arg1	the					965:967	the	965:967	the	965:967	The data was submitted to the Normality and Homogeneity tests.
36740600	3	86	theme	Infrared	642:649	arg1	Spectroscopy					651:662	Infrared Spectroscopy	642:662	Infrared Spectroscopy (FTIR)	642:669	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	3	86	theme	Infrared	642:649	arg1	FTIR					665:668	FTIR	665:668	FTIR	665:668	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	14	87	theme	CX	1768:1769	arg1	group					1771:1775	the CX group the highest contact angle and CXHA the lowest (p < 0.05)	1764:1832	group	1771:1775	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	2	88	theme	wt	543:544	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	88	theme	wt	543:544	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	18	89	theme	morphological	2190:2202	arg1	properties					2247:2256	mechanical and biological properties	2221:2256	mechanical and biological properties favorable for the regeneration process	2221:2295	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	18	89	theme	morphological	2190:2202	arg1	characteristics					2204:2218	morphological characteristics	2190:2218	morphological characteristics	2190:2218	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	0	90	theme	complex	118:124	arg1	regeneration					126:137	dentin-pulp complex regeneration	106:137	dentin-pulp complex regeneration	106:137	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold associated with mesenchymal stem cells for dentin-pulp complex regeneration.
36740600	17	91	theme	%	2074:2074	arg1	scaffold					2092:2099	CXHAGO 1.0% and CXHAGO 1.5% scaffold	2064:2099	CXHAGO 1.0% and CXHAGO 1.5% scaffold	2064:2099	MTT test showed high cell viability in CXHAGO 1.0% and CXHAGO 1.5% scaffold.
36740600	15	92	theme	other	1907:1911	arg1	compositions					1913:1924	other compositions	1907:1924	other compositions	1907:1924	1.0 wt% GO significantly increased the compressive strength compared to other compositions.
36740600	6	93	theme	Homogeneity	983:993	arg1	tests					995:999	Homogeneity tests	983:999	Homogeneity tests	983:999	The data was submitted to the Normality and Homogeneity tests.
36740600	17	94	theme	MTT	2025:2027	arg1	test					2029:2032	MTT test	2025:2032	MTT test	2025:2032	MTT test showed high cell viability in CXHAGO 1.0% and CXHAGO 1.5% scaffold.
36740600	7	95	dep	In	1002:1003	arg1	vitro					1005:1009	vitro	1005:1009	vitro	1005:1009	In vitro Indirect Cytotoxicity assay data was statistically analyzed by ANOVA two-way, followed by Tukey's test (α = 0.05).
36740600	17	96	theme	%	2090:2090	arg1	scaffold					2092:2099	CXHAGO 1.0% and CXHAGO 1.5% scaffold	2064:2099	CXHAGO 1.0% and CXHAGO 1.5% scaffold	2064:2099	MTT test showed high cell viability in CXHAGO 1.0% and CXHAGO 1.5% scaffold.
36740600	4	97	theme	mechanical	784:793	arg1	properties					795:804	The mechanical properties	780:804	The mechanical properties	780:804	The mechanical properties were assessed by compressive strength.
36740600	2	98	theme	Graphene	485:492	arg1	Oxides					494:499	Graphene Oxides	485:499	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	0	99	theme	oxide	41:45	arg1	scaffold					54:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold	0:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold	0:61	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold associated with mesenchymal stem cells for dentin-pulp complex regeneration.
36740600	15	100	theme	compressive	1874:1884	arg1	strength					1886:1893	the compressive strength	1870:1893	the compressive strength	1870:1893	1.0 wt% GO significantly increased the compressive strength compared to other compositions.
36740600	12	101	theme	CX	1544:1545	arg1	degradation					1547:1557	the CX degradation	1540:1557	the CX degradation by depolymerization	1540:1577	In the TGA it was observed the mass loss associated with the CX degradation by depolymerization.
36740600	18	102	theme	CXHAGO	2102:2107	arg1	scaffolds					2109:2117	CXHAGO scaffolds	2102:2117	CXHAGO scaffolds	2102:2117	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	2	103	theme	wt	525:526	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	103	theme	wt	525:526	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	13	104	theme	SEM	1580:1582	arg1	analysis					1584:1591	SEM analysis	1580:1591	SEM analysis	1580:1591	SEM analysis showed pores in the scaffolds, in addition to HA incorporated and adhered to the polymer.
36740600	17	105	theme	cell	2046:2049	arg1	viability					2051:2059	high cell viability	2041:2059	high cell viability	2041:2059	MTT test showed high cell viability in CXHAGO 1.0% and CXHAGO 1.5% scaffold.
36740600	9	106	theme	Hydroxyapatite	1280:1293	arg1	peaks					1300:1304	Hydroxyapatite (HA) peaks	1280:1304	Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%	1280:1354	XRD showed the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%.
36740600	18	107	theme	dentistry	2150:2158	arg1	application					2160:2170	regenerative dentistry application	2137:2170	regenerative dentistry application	2137:2170	CXHAGO scaffolds are promising for regenerative dentistry application because they have morphological characteristics, mechanical and biological properties favorable for the regeneration process.
36740600	3	108	theme	Raman	672:676	arg1	spectroscopy					678:689	Raman spectroscopy	672:689	Raman spectroscopy	672:689	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	14	109	dep	group	1771:1775	arg1	angle					1797:1801	the highest contact angle	1777:1801	the highest contact angle	1777:1801	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	14	109	dep	group	1771:1775	arg1	lowest					1816:1821	lowest	1816:1821	lowest	1816:1821	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	14	109	dep	group	1771:1775	arg1	<					1826:1826	p < 0.05	1824:1831	p < 0.05	1824:1831	Contact angle test showed that scaffolds have a hydrophilic characteristic, with the CX group the highest contact angle and CXHA the lowest (p < 0.05).
36740600	2	110	theme	wt	534:535	arg1	%					545:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	0.5 wt%; 1.0 wt%; 1.5 wt%	521:545	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	110	theme	wt	534:535	arg1	concentration					506:518	different Graphene Oxides (GO) concentration	475:518	different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	475:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	8	111	dep	analyzed	1189:1196	arg1	followed					1216:1223	followed	1216:1223	followed by Tukey's test (α = 0.05)	1216:1250	Compressive strength and contact angle data were statistically analyzed by one-way ANOVA, followed by Tukey's test (α = 0.05).
36740600	0	112	theme	stem	91:94	arg1	cells					96:100	mesenchymal stem cells	79:100	mesenchymal stem cells for dentin-pulp complex regeneration	79:137	Chitosan/Xanthan/Hydroxyapatite-graphene oxide porous scaffold associated with mesenchymal stem cells for dentin-pulp complex regeneration.
36740600	9	113	theme	peaks	1300:1304	arg1	presence					1268:1275	the presence	1264:1275	the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%	1264:1354	XRD showed the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%.
36740600	2	114	theme	Hydroxyapatite-Graphene	418:440	arg1	HA-GO					449:453	HA-GO	449:453	HA-GO	449:453	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	2	114	theme	Hydroxyapatite-Graphene	418:440	arg1	Oxide					442:446	Hydroxyapatite-Graphene Oxide	418:446	Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%)	418:546	The chitosan-xanthan gum (CX) complex was associated with Hydroxyapatite-Graphene Oxide (HA-GO) nanocomposite with different Graphene Oxides (GO) concentration (0.5 wt%; 1.0 wt%; 1.5 wt%).
36740600	9	115	from	structures	1313:1322	arg1	presence					1268:1275	the presence	1264:1275	the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%	1264:1354	XRD showed the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%.
36740600	1	116	theme	stem	309:312	arg1	cells					314:318	mesenchymal stem cells	297:318	mesenchymal stem cells for regenerative dentistry application	297:357	The aim of this paper was to synthesize and characterize polymeric scaffolds of Chitosan/Xanthan/Hydroxyapatite-Graphene Oxide nanocomposite associated with mesenchymal stem cells for regenerative dentistry application.
36740600	3	117	theme	scaffolds	553:561	arg1	characterizations					563:579	The scaffolds characterizations	549:579	The scaffolds characterizations	549:579	The scaffolds characterizations were performed by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and contact angle.
36740600	9	118	theme	CXHAGO	1330:1335	arg1	%					1340:1340	CXHAGO 0.5%	1330:1340	CXHAGO 0.5%	1330:1340	XRD showed the presence of Hydroxyapatite (HA) peaks in the structures CXHA, CXHAGO 0.5%,1.0% and 1.5%.
36712950	4	0	theme	bonded	733:738	arg1	teeth					740:744	The bonded teeth	729:744	The bonded teeth of each group	729:758	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	8	1	from	expression	1682:1691	arg1	<					1667:1667	P < 0.05	1665:1672	P < 0.05	1665:1672	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	1	from	expression	1682:1691	arg1	layers					1657:1662	the hybrid layers	1646:1662	the hybrid layers (P < 0.05)	1646:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	2	theme	higher	1614:1619	arg1	modulus					1621:1627	significantly higher modulus	1600:1627	significantly higher modulus of elasticity in the hybrid layers (P < 0.05)	1600:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	7	3	theme	two-way	1281:1287	arg1	ANOVA					1289:1293	two-way ANOVA	1281:1293	two-way ANOVA	1281:1293	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	6	4	theme	selected	1096:1103	arg1	area					1105:1108	a selected area	1094:1108	a selected area	1094:1108	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	8	5	theme	statistical	1491:1501	arg1	significance					1503:1514	a statistical significance	1489:1514	a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1489:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	5	theme	statistical	1491:1501	arg1	>					1519:1519	P > 0.05	1517:1524	P > 0.05	1517:1524	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	7	6	theme	comparison	1312:1321	arg1	test					1323:1326	LSD multiple comparison test	1299:1326	LSD multiple comparison test (P < 0.05)	1299:1337	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	7	6	theme	comparison	1312:1321	arg1	<					1331:1331	P < 0.05	1329:1336	P < 0.05	1329:1336	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	6	7	theme	bonding	999:1005	arg1	interface					1007:1015	bonding interface	999:1015	bonding interface	999:1015	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	2	8	theme	third	299:303	arg1	molars					305:310	non-carious human third molars	281:310	non-carious human third molars	281:310	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	7	9	theme	LSD	1299:1301	arg1	test					1323:1326	LSD multiple comparison test	1299:1326	LSD multiple comparison test (P < 0.05)	1299:1337	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	7	9	theme	LSD	1299:1301	arg1	<					1331:1331	P < 0.05	1329:1336	P < 0.05	1329:1336	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	8	10	theme	hybrid	1650:1655	arg1	<					1667:1667	P < 0.05	1665:1672	P < 0.05	1665:1672	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	10	theme	hybrid	1650:1655	arg1	layers					1657:1662	the hybrid layers	1646:1662	the hybrid layers (P < 0.05)	1646:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	3	11	theme	CS	488:489	arg1	groups					506:511	the DW, CS, CHX, and CONP groups	480:511	the DW, CS, CHX, and CONP groups	480:511	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	9	12	contain	had	1871:1873	arg1	CONPs					1865:1869	the CONPs	1861:1869	the CONPs	1861:1869	Conclusion Altogether, the CONPs had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability.
36712950	9	12	contain	had	1871:1873	arg2	potential					1879:1887	the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability	1875:1982	the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability	1875:1982	Conclusion Altogether, the CONPs had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability.
36712950	6	13	theme	endogenous	1166:1175	arg1	activity					1188:1195	the endogenous gelatinase activity	1162:1195	the endogenous gelatinase activity	1162:1195	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	5	14	theme	bonded	900:905	arg1	beams					907:911	the bonded beams	896:911	the bonded beams	896:911	The bonded teeth of each group were then cut into the bonded beams.
36712950	3	15	theme	DW	484:485	arg1	groups					506:511	the DW, CS, CHX, and CONP groups	480:511	the DW, CS, CHX, and CONP groups	480:511	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	9	16	theme	dentin	1901:1906	arg1	primer					1908:1913	a dentin primer	1899:1913	a dentin primer	1899:1913	Conclusion Altogether, the CONPs had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability.
36712950	9	17	dep	Conclusion	1838:1847	arg1	had					1871:1873	had	1871:1873	had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability	1871:1982	Conclusion Altogether, the CONPs had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability.
36712950	8	18	dep	h.	1563:1564	arg1	presented					1590:1598	presented	1590:1598	presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1590:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	1	19	theme	bonding	228:234	arg1	interfaces					236:245	resin-dentin bonding interfaces	215:245	resin-dentin bonding interfaces	215:245	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	4	20	theme	group	754:758	arg1	teeth					740:744	The bonded teeth	729:744	The bonded teeth of each group	729:758	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	3	21	theme	100	636:638	arg1	mg/L					640:643	mg/L	640:643	mg/L	640:643	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	3	22	theme	CHX	492:494	arg1	groups					506:511	the DW, CS, CHX, and CONP groups	480:511	the DW, CS, CHX, and CONP groups	480:511	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	1	23	theme	dentin	120:125	arg1	pretreatment					127:138	dentin pretreatment	120:138	dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs)	120:192	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	2	24	dep	groups	344:349	arg1	group					459:463	a CONP group	452:463	a CONP group	452:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	24	dep	groups	344:349	arg1	CS					409:410	CS	409:410	CS	409:410	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	24	dep	groups	344:349	arg1	groups					344:349	four groups	339:349	four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group	339:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	24	dep	groups	344:349	arg1	CHX					437:439	CHX	437:439	CHX	437:439	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	24	dep	groups	344:349	arg1	group					390:394	a de-ionized water (DW) group	366:394	a de-ionized water (DW) group	366:394	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	24	dep	groups	344:349	arg1	group					442:446	a chlorhexidine (CHX) group	420:446	a chlorhexidine (CHX) group	420:446	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	24	dep	groups	344:349	arg1	n					352:352	n	352:352	n = 20 each	352:362	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	24	dep	groups	344:349	arg1	group					413:417	a chitosan (CS) group	397:417	a chitosan (CS) group	397:417	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	6	25	theme	nanoleakage	1126:1136	arg1	analysis					1138:1145	interfacial nanoleakage analysis	1114:1145	interfacial nanoleakage analysis	1114:1145	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	2	26	theme	non-carious	281:291	arg1	molars					305:310	non-carious human third molars	281:310	non-carious human third molars	281:310	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	6	27	theme	force	1027:1031	arg1	microscope					1033:1042	atomic force microscope	1020:1042	atomic force microscope	1020:1042	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	6	28	from	percentage	1060:1069	arg1	area					1105:1108	a selected area	1094:1108	a selected area	1094:1108	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	6	29	theme	in-situ	1230:1236	arg1	analysis					1247:1254	in-situ zymogram analysis	1230:1254	in-situ zymogram analysis	1230:1254	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	3	30	dep	suspension	610:619	arg1	prepared					622:629	prepared	622:629	prepared with 100 mg/L oleuropein	622:654	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	8	31	theme	inhibitory	1731:1740	arg1	effect					1742:1747	better inhibitory effect	1724:1747	better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1724:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	1	32	theme	oleuropein	161:170	arg1	CONPs					187:191	CONPs	187:191	CONPs	187:191	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	1	32	theme	oleuropein	161:170	arg1	nanoparticles					172:184	chitosan-loaded oleuropein nanoparticles	145:184	chitosan-loaded oleuropein nanoparticles (CONPs)	145:192	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	8	33	theme	lower	1676:1680	arg1	expression					1682:1691	lower expression	1676:1691	lower expression of nanoleakage (P < 0.05)	1676:1717	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	3	34	theme	universal	688:696	arg1	composites					717:726	the universal adhesive and resin composites	684:726	the universal adhesive and resin composites	684:726	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	9	35	theme	binding	1965:1971	arg1	durability					1973:1982	the dentin-resin binding durability	1948:1982	the dentin-resin binding durability	1948:1982	Conclusion Altogether, the CONPs had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability.
36712950	8	36	theme	nanoleakage	1696:1706	arg1	effect					1742:1747	better inhibitory effect	1724:1747	better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1724:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	36	theme	nanoleakage	1696:1706	arg1	modulus					1621:1627	significantly higher modulus	1600:1627	significantly higher modulus of elasticity in the hybrid layers (P < 0.05)	1600:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	36	theme	nanoleakage	1696:1706	arg1	expression					1682:1691	lower expression	1676:1691	lower expression of nanoleakage (P < 0.05)	1676:1717	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	2	37	theme	DW	386:387	arg1	group					390:394	a de-ionized water (DW) group	366:394	a de-ionized water (DW) group	366:394	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	37	theme	DW	386:387	arg1	groups					344:349	four groups	339:349	four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group	339:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	0	38	theme	Bonding	76:82	arg1	Durability					55:64	the Durability	51:64	the Durability of Dentin Bonding	51:82	Evaluation of Chitosan-Oleuropein Nanoparticles on the Durability of Dentin Bonding.
36712950	2	39	theme	water	379:383	arg1	group					390:394	a de-ionized water (DW) group	366:394	a de-ionized water (DW) group	366:394	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	39	theme	water	379:383	arg1	groups					344:349	four groups	339:349	four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group	339:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	40	dep	20	356:357	arg1	each					359:362	each	359:362	each	359:362	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	0	41	theme	Chitosan-Oleuropein	14:32	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Chitosan-Oleuropein	0:32	Evaluation of Chitosan-Oleuropein Nanoparticles on the Durability of Dentin Bonding.
36712950	2	42	theme	chlorhexidine	422:434	arg1	group					442:446	a chlorhexidine (CHX) group	420:446	a chlorhexidine (CHX) group	420:446	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	42	theme	chlorhexidine	422:434	arg1	CHX					437:439	CHX	437:439	CHX	437:439	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	42	theme	chlorhexidine	422:434	arg1	groups					344:349	four groups	339:349	four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group	339:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	0	43	theme	Dentin	69:74	arg1	Bonding					76:82	Dentin Bonding	69:82	Dentin Bonding	69:82	Evaluation of Chitosan-Oleuropein Nanoparticles on the Durability of Dentin Bonding.
36712950	3	44	theme	de-ionized	534:543	arg1	water					545:549	de-ionized water	534:549	de-ionized water	534:549	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	8	45	theme	metalloproteinases	1759:1776	arg1	effect					1742:1747	better inhibitory effect	1724:1747	better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1724:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	45	theme	metalloproteinases	1759:1776	arg1	modulus					1621:1627	significantly higher modulus	1600:1627	significantly higher modulus of elasticity in the hybrid layers (P < 0.05)	1600:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	45	theme	metalloproteinases	1759:1776	arg1	expression					1682:1691	lower expression	1676:1691	lower expression of nanoleakage (P < 0.05)	1676:1717	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	46	theme	other	1444:1448	arg1	groups					1456:1461	the other three groups	1440:1461	the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1440:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	1	47	from	effects	109:115	arg1	durability					201:210	the durability	197:210	the durability of resin-dentin bonding interfaces	197:245	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	1	48	theme	interfaces	236:245	arg1	durability					201:210	the durability	197:210	the durability of resin-dentin bonding interfaces	197:245	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	3	49	theme	mg/L	556:559	arg1	solution					564:571	1.0 mg/L CS solution	552:571	1.0 mg/L CS solution	552:571	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	7	50	theme	P	1329:1329	arg1	test					1323:1326	LSD multiple comparison test	1299:1326	LSD multiple comparison test (P < 0.05)	1299:1337	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	7	50	theme	P	1329:1329	arg1	<					1331:1331	P < 0.05	1329:1336	P < 0.05	1329:1336	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	6	51	theme	microtensile	932:943	arg1	μTBS					960:963	μTBS	960:963	μTBS	960:963	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	6	51	theme	microtensile	932:943	arg1	strength					950:957	their microtensile bond strength	926:957	their microtensile bond strength (μTBS)	926:964	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	8	52	theme	P	1425:1425	arg1	μTBS					1419:1422	better μTBS	1412:1422	better μTBS (P < 0.05)	1412:1433	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	52	theme	P	1425:1425	arg1	<					1427:1427	P < 0.05	1425:1432	P < 0.05	1425:1432	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	3	53	theme	chlorhexidine	577:589	arg1	solution					591:598	2% chlorhexidine solution	574:598	2% chlorhexidine solution	574:598	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	6	54	theme	interfacial	1114:1124	arg1	analysis					1138:1145	interfacial nanoleakage analysis	1114:1145	interfacial nanoleakage analysis	1114:1145	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	3	55	theme	2	574:574	arg1	%					575:575	%	575:575	%	575:575	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	8	56	theme	elasticity	1632:1641	arg1	effect					1742:1747	better inhibitory effect	1724:1747	better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1724:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	56	theme	elasticity	1632:1641	arg1	modulus					1621:1627	significantly higher modulus	1600:1627	significantly higher modulus of elasticity in the hybrid layers (P < 0.05)	1600:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	56	theme	elasticity	1632:1641	arg1	expression					1682:1691	lower expression	1676:1691	lower expression of nanoleakage (P < 0.05)	1676:1717	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	7	57	theme	multiple	1303:1310	arg1	test					1323:1326	LSD multiple comparison test	1299:1326	LSD multiple comparison test (P < 0.05)	1299:1337	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	7	57	theme	multiple	1303:1310	arg1	<					1331:1331	P < 0.05	1329:1336	P < 0.05	1329:1336	Data were analyzed with two-way ANOVA and LSD multiple comparison test (P < 0.05).
36712950	6	58	theme	interface	1007:1015	arg1	characteristics					980:994	the characteristics	976:994	the characteristics of bonding interface	976:1015	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	3	59	theme	CONP	605:608	arg1	suspension					610:619	CONP suspension	605:619	CONP suspension (prepared with 100 mg/L oleuropein)	605:655	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	2	60	dep	freshly	263:269	arg1	Eighty					256:261	Eighty	256:261	Eighty	256:261	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	6	61	theme	particles	1081:1089	arg1	percentage					1060:1069	the percentage	1056:1069	the percentage of silver particles in a selected area	1056:1108	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	6	62	theme	silver	1074:1079	arg1	particles					1081:1089	silver particles	1074:1089	silver particles	1074:1089	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	6	63	theme	gelatinase	1177:1186	arg1	activity					1188:1195	the endogenous gelatinase activity	1162:1195	the endogenous gelatinase activity	1162:1195	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	8	64	theme	P	1517:1517	arg1	significance					1503:1514	a statistical significance	1489:1514	a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1489:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	64	theme	P	1517:1517	arg1	>					1519:1519	P > 0.05	1517:1524	P > 0.05	1517:1524	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	1	65	theme	resin-dentin	215:226	arg1	interfaces					236:245	resin-dentin bonding interfaces	215:245	resin-dentin bonding interfaces	215:245	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	9	66	theme	dentin-resin	1952:1963	arg1	durability					1973:1982	the dentin-resin binding durability	1948:1982	the dentin-resin binding durability	1948:1982	Conclusion Altogether, the CONPs had the potential to act as a dentin primer, which could effectively improve the dentin-resin binding durability.
36712950	4	67	dep	subgroups	791:799	arg1	subgroup					815:822	an immediate subgroup	802:822	an immediate subgroup	802:822	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	4	67	dep	subgroups	791:799	arg1	subgroups					791:799	two subgroups	787:799	two subgroups: an immediate subgroup and an aged subgroup	787:843	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	4	67	dep	subgroups	791:799	arg1	subgroup					836:843	an aged subgroup	828:843	an aged subgroup	828:843	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	8	68	theme	P	1665:1665	arg1	<					1667:1667	P < 0.05	1665:1672	P < 0.05	1665:1672	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	68	theme	P	1665:1665	arg1	layers					1657:1662	the hybrid layers	1646:1662	the hybrid layers (P < 0.05)	1646:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	3	69	theme	mg/L	640:643	arg1	oleuropein					645:654	100 mg/L oleuropein	636:654	100 mg/L oleuropein	636:654	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	2	70	theme	human	293:297	arg1	molars					305:310	non-carious human third molars	281:310	non-carious human third molars	281:310	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	8	71	theme	CONP	1534:1537	arg1	groups					1547:1552	the CONP and CHX groups	1530:1552	groups	1547:1552	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	1	72	theme	pretreatment	127:138	arg1	effects					109:115	the effects	105:115	the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces	105:245	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	6	73	theme	zymogram	1238:1245	arg1	analysis					1247:1254	in-situ zymogram analysis	1230:1254	in-situ zymogram analysis	1230:1254	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	3	74	theme	CONP	501:504	arg1	groups					506:511	the DW, CS, CHX, and CONP groups	480:511	the DW, CS, CHX, and CONP groups	480:511	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	8	75	theme	CHX	1543:1545	arg1	groups					1547:1552	the CONP and CHX groups	1530:1552	groups	1547:1552	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	1	76	theme	chitosan-loaded	145:159	arg1	CONPs					187:191	CONPs	187:191	CONPs	187:191	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	1	76	theme	chitosan-loaded	145:159	arg1	nanoparticles					172:184	chitosan-loaded oleuropein nanoparticles	145:184	chitosan-loaded oleuropein nanoparticles (CONPs)	145:192	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	3	77	theme	resin	711:715	arg1	composites					717:726	the universal adhesive and resin composites	684:726	the universal adhesive and resin composites	684:726	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	8	78	from	effect	1742:1747	arg1	<					1667:1667	P < 0.05	1665:1672	P < 0.05	1665:1672	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	78	from	effect	1742:1747	arg1	layers					1657:1662	the hybrid layers	1646:1662	the hybrid layers (P < 0.05)	1646:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	6	79	theme	atomic	1020:1025	arg1	microscope					1033:1042	atomic force microscope	1020:1042	atomic force microscope	1020:1042	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	8	80	theme	P	1709:1709	arg1	nanoleakage					1696:1706	nanoleakage	1696:1706	nanoleakage (P < 0.05)	1696:1717	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	80	theme	P	1709:1709	arg1	<					1711:1711	P < 0.05	1709:1716	P < 0.05	1709:1716	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	4	81	theme	aged	831:834	arg1	subgroups					791:799	two subgroups	787:799	two subgroups: an immediate subgroup and an aged subgroup	787:843	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	4	81	theme	aged	831:834	arg1	subgroup					836:843	an aged subgroup	828:843	an aged subgroup	828:843	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	3	82	from	dentin	470:475	arg1	groups					506:511	the DW, CS, CHX, and CONP groups	480:511	the DW, CS, CHX, and CONP groups	480:511	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	3	83	theme	adhesive	698:705	arg1	composites					717:726	the universal adhesive and resin composites	684:726	the universal adhesive and resin composites	684:726	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	6	84	theme	bonding	1208:1214	arg1	interface					1216:1224	the bonding interface	1204:1224	the bonding interface for in-situ zymogram analysis	1204:1254	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	2	85	theme	CONP	454:457	arg1	groups					344:349	four groups	339:349	four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group	339:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	85	theme	CONP	454:457	arg1	group					459:463	a CONP group	452:463	a CONP group	452:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	1	86	with	pretreatment	127:138	arg1	CONPs					187:191	CONPs	187:191	CONPs	187:191	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	1	86	with	pretreatment	127:138	arg1	nanoparticles					172:184	chitosan-loaded oleuropein nanoparticles	145:184	chitosan-loaded oleuropein nanoparticles (CONPs)	145:192	Purpose To evaluate the effects of dentin pretreatment with chitosan-loaded oleuropein nanoparticles (CONPs) on the durability of resin-dentin bonding interfaces.
36712950	8	87	theme	other	1787:1791	arg1	groups					1799:1804	the other three groups	1783:1804	the other three groups before and after thermocycling	1783:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	4	88	theme	immediate	805:813	arg1	subgroup					815:822	an immediate subgroup	802:822	an immediate subgroup	802:822	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	4	88	theme	immediate	805:813	arg1	subgroups					791:799	two subgroups	787:799	two subgroups: an immediate subgroup and an aged subgroup	787:843	The bonded teeth of each group were randomly divided into two subgroups: an immediate subgroup and an aged subgroup.
36712950	8	89	theme	better	1724:1729	arg1	effect					1742:1747	better inhibitory effect	1724:1747	better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1724:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	90	theme	CONP	1579:1582	arg1	group					1584:1588	the CONP group	1575:1588	the CONP group	1575:1588	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	91	from	elasticity	1632:1641	arg1	<					1667:1667	P < 0.05	1665:1672	P < 0.05	1665:1672	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	91	from	elasticity	1632:1641	arg1	layers					1657:1662	the hybrid layers	1646:1662	the hybrid layers (P < 0.05)	1646:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	2	92	theme	de-ionized	368:377	arg1	group					390:394	a de-ionized water (DW) group	366:394	a de-ionized water (DW) group	366:394	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	92	theme	de-ionized	368:377	arg1	groups					344:349	four groups	339:349	four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group	339:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	93	theme	chitosan	399:406	arg1	CS					409:410	CS	409:410	CS	409:410	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	93	theme	chitosan	399:406	arg1	group					413:417	a chitosan (CS) group	397:417	a chitosan (CS) group	397:417	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	2	93	theme	chitosan	399:406	arg1	groups					344:349	four groups	339:349	four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group	339:463	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	8	94	from	modulus	1621:1627	arg1	<					1667:1667	P < 0.05	1665:1672	P < 0.05	1665:1672	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	94	from	modulus	1621:1627	arg1	layers					1657:1662	the hybrid layers	1646:1662	the hybrid layers (P < 0.05)	1646:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	2	95	dep	=	354:354	arg1	20					356:357	20	356:357	20	356:357	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	8	96	theme	matrix	1752:1757	arg1	metalloproteinases					1759:1776	matrix metalloproteinases	1752:1776	matrix metalloproteinases	1752:1776	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	97	from	layers	1657:1662	arg1	effect					1742:1747	better inhibitory effect	1724:1747	better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling	1724:1835	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	97	from	layers	1657:1662	arg1	modulus					1621:1627	significantly higher modulus	1600:1627	significantly higher modulus of elasticity in the hybrid layers (P < 0.05)	1600:1673	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	97	from	layers	1657:1662	arg1	expression					1682:1691	lower expression	1676:1691	lower expression of nanoleakage (P < 0.05)	1676:1717	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	98	from	significance	1503:1514	arg1	groups					1547:1552	the CONP and CHX groups	1530:1552	groups	1547:1552	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	5	99	theme	group	871:875	arg1	teeth					857:861	The bonded teeth	846:861	The bonded teeth of each group	846:875	The bonded teeth of each group were then cut into the bonded beams.
36712950	8	100	theme	better	1412:1417	arg1	μTBS					1419:1422	better μTBS	1412:1422	better μTBS (P < 0.05)	1412:1433	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	100	theme	better	1412:1417	arg1	<					1427:1427	P < 0.05	1425:1432	P < 0.05	1425:1432	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	3	101	theme	CS	561:562	arg1	solution					564:571	1.0 mg/L CS solution	552:571	1.0 mg/L CS solution	552:571	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	2	102	theme	=	354:354	arg1	n					352:352	n	352:352	n = 20 each	352:362	Methods Eighty freshly extracted non-carious human third molars were randomly divided into four groups (n = 20 each): a de-ionized water (DW) group, a chitosan (CS) group, a chlorhexidine (CHX) group and a CONP group.
36712950	3	103	theme	1.0	552:554	arg1	mg/L					556:559	mg/L	556:559	mg/L	556:559	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	3	104	dep	pretreated	518:527	arg1	followed					672:679	followed	672:679	followed by the universal adhesive and resin composites	672:726	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	5	105	theme	bonded	850:855	arg1	teeth					857:861	The bonded teeth	846:861	The bonded teeth of each group	846:875	The bonded teeth of each group were then cut into the bonded beams.
36712950	6	106	theme	bond	945:948	arg1	μTBS					960:963	μTBS	960:963	μTBS	960:963	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	6	106	theme	bond	945:948	arg1	strength					950:957	their microtensile bond strength	926:957	their microtensile bond strength (μTBS)	926:964	We measured their microtensile bond strength (μTBS), observed the characteristics of bonding interface by atomic force microscope, calculated the percentage of silver particles in a selected area for interfacial nanoleakage analysis, and evaluated the endogenous gelatinase activity within the bonding interface for in-situ zymogram analysis.
36712950	3	107	theme	%	575:575	arg1	solution					591:598	2% chlorhexidine solution	574:598	2% chlorhexidine solution	574:598	The dentin in the DW, CS, CHX, and CONP groups were pretreated with de-ionized water, 1.0 mg/L CS solution, 2% chlorhexidine solution, and CONP suspension (prepared with 100 mg/L oleuropein), respectively, followed by the universal adhesive and resin composites.
36712950	8	108	dep	Regardless	1348:1357	arg1	CONP					1397:1400	CONP	1397:1400	CONP	1397:1400	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	108	dep	Regardless	1348:1357	arg1	h					1371:1371	24 h	1368:1371	24 h	1368:1371	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36712950	8	108	dep	Regardless	1348:1357	arg1	thermocycling					1382:1394	thermocycling	1382:1394	thermocycling	1382:1394	Results Regardless of after 24 h or after thermocycling, CONP exhibited better μTBS (P < 0.05) than the other three groups except that there was not a statistical significance (P > 0.05) in the CONP and CHX groups after 24 h. Besides, the CONP group presented significantly higher modulus of elasticity in the hybrid layers (P < 0.05), lower expression of nanoleakage (P < 0.05), and better inhibitory effect of matrix metalloproteinases than the other three groups before and after thermocycling.
36197573	9	0	theme	patients	1165:1172	arg1	cohort					1155:1160	SIBO-positive cohort	1141:1160	SIBO-positive cohort of patients	1141:1172	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	7	1	theme	intolerance	923:933	arg1	frequency					878:886	higher frequency	871:886	higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002)	871:1034	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	2	theme	patients	838:845	arg1	24/56					823:827	24/56	823:827	24/56	823:827	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	2	theme	patients	838:845	arg1	%					832:832	43%	830:832	43%	830:832	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	2	theme	patients	838:845	arg1	patients					838:845	patients	838:845	patients	838:845	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	1	3	theme	small	252:256	arg1	bowel					258:262	the small bowel	248:262	the small bowel	248:262	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	8	4	theme	Activity	1048:1055	arg1	Score					1057:1061	Mean NAFLD Activity Score	1037:1061	Mean NAFLD Activity Score in SIBO patients (n = 18)	1037:1087	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	7	5	dep	RESULTS	779:785	arg1	positive					811:818	positive	811:818	positive	811:818	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	5	dep	RESULTS	779:785	arg1	associated					855:864	associated	855:864	was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002)	851:1034	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	3	6	theme	liver	489:493	arg1	pathology					495:503	liver pathology	489:503	liver pathology	489:503	The purpose of this study is to evaluate the incidence of SIBO after bariatric bypass procedures, connection between SIBO, symptoms, comorbidities, and liver pathology.
36197573	2	7	theme	complications	322:334	arg1	risk					297:300	increased risk	287:300	increased risk of SIBO and related complications	287:334	Obese patients are at increased risk of SIBO and related complications.
36197573	8	8	theme	SIBO	1066:1069	arg1	patients					1071:1078	SIBO patients	1066:1078	SIBO patients	1066:1078	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	9	9	theme	dose	1296:1299	arg1	application					1301:1311	subtherapeutic dose application	1281:1311	subtherapeutic dose application	1281:1311	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	2	10	theme	increased	287:295	arg1	risk					297:300	increased risk	287:300	increased risk of SIBO and related complications	287:334	Obese patients are at increased risk of SIBO and related complications.
36197573	1	11	theme	BACKGROUND	116:125	arg1	SIBO					166:169	SIBO	166:169	SIBO	166:169	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	11	theme	BACKGROUND	116:125	arg1	overgrowth					154:163	BACKGROUND Small intestinal bacterial overgrowth	116:163	BACKGROUND Small intestinal bacterial overgrowth (SIBO)	116:170	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	11	theme	BACKGROUND	116:125	arg1	growth					199:204	an excessive growth	186:204	an excessive growth	186:204	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	11	theme	BACKGROUND	116:125	arg1	composition					221:231	changed composition	213:231	changed composition of bacteria	213:243	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	0	12	theme	Gastric	100:106	arg1	Roux-en-Y					55:63	Roux-en-Y	55:63	Roux-en-Y Gastric Bypass and One-Anastomosis Gastric	55:106	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	12	13	theme	underlying	1680:1689	arg1	disease					1691:1697	the underlying disease	1676:1697	the underlying disease	1676:1697	Mandatory SIBO screening and appropriate treatment would affect the clinical outcome of the underlying disease, improve it significantly, and prevent the development of its complications.
36197573	4	14	with	test	551:554	arg1	substrate					569:577	glucose substrate	561:577	glucose substrate (25 g/200 ml of water)	561:600	METHODS Patients underwent a hydrogen breath test with glucose substrate (25 g/200 ml of water).
36197573	8	15	theme	Mean	1037:1040	arg1	Score					1057:1061	Mean NAFLD Activity Score	1037:1061	Mean NAFLD Activity Score in SIBO patients (n = 18)	1037:1087	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	12	16	theme	disease	1691:1697	arg1	outcome					1665:1671	the clinical outcome	1652:1671	the clinical outcome of the underlying disease	1652:1697	Mandatory SIBO screening and appropriate treatment would affect the clinical outcome of the underlying disease, improve it significantly, and prevent the development of its complications.
36197573	9	17	theme	important	1191:1199	arg1	trend					1201:1205	a statistically important trend	1175:1205	a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application	1175:1311	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	10	18	theme	bariatric	1369:1377	arg1	surgery					1379:1385	bariatric surgery bypass procedures	1369:1403	bariatric surgery bypass procedures	1369:1403	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	7	19	theme	diabetes	1015:1022	arg1	frequency					878:886	higher frequency	871:886	higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002)	871:1034	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	6	20	theme	liver	763:767	arg1	biopsies					769:776	liver biopsies	763:776	liver biopsies	763:776	In 45 patients, the NAFLD Activity Score was evaluated in liver biopsies.
36197573	8	21	from	3.33	1093:1096	arg1	n = 27					1129:1134	n = 27	1129:1134	n = 27	1129:1134	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	8	21	from	3.33	1093:1096	arg1	patients					1119:1126	non-SIBO patients	1110:1126	non-SIBO patients (n = 27)	1110:1135	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	0	22	theme	Intestinal	6:15	arg1	Overgrowth					27:36	Small Intestinal Bacterial Overgrowth	0:36	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric	0:106	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	10	23	theme	SIBO	1358:1361	arg1	high					1419:1422	high	1419:1422	high	1419:1422	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	10	23	theme	SIBO	1358:1361	arg1	%					1427:1427	43%	1425:1427	43%	1425:1427	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	10	23	theme	SIBO	1358:1361	arg1	incidence					1345:1353	The incidence	1341:1353	The incidence of SIBO after bariatric surgery bypass procedures	1341:1403	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	7	24	theme	defecation	891:900	arg1	frequency					878:886	higher frequency	871:886	higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002)	871:1034	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	11	25	theme	reduced	1543:1549	arg1	response					1551:1558	reduced response	1543:1558	reduced response to the application of LMWH	1543:1585	The results of our study conclude that diagnosis cannot be set based on specific symptom and SIBO is related to reduced response to the application of LMWH.
36197573	3	26	theme	bariatric	406:414	arg1	procedures					423:432	bariatric bypass procedures	406:432	bariatric bypass procedures	406:432	The purpose of this study is to evaluate the incidence of SIBO after bariatric bypass procedures, connection between SIBO, symptoms, comorbidities, and liver pathology.
36197573	0	27	theme	Small	0:4	arg1	Overgrowth					27:36	Small Intestinal Bacterial Overgrowth	0:36	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric	0:106	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	4	28	theme	breath	544:549	arg1	test					551:554	a hydrogen breath test	533:554	a hydrogen breath test with glucose substrate (25 g/200 ml of water)	533:600	METHODS Patients underwent a hydrogen breath test with glucose substrate (25 g/200 ml of water).
36197573	5	29	theme	anthropometric	620:633	arg1	data					635:638	anthropometric data	620:638	anthropometric data	620:638	The demographic, anthropometric data, comorbidities, and symptoms were analysed with a questionnaire.
36197573	7	30	theme	higher	871:876	arg1	frequency					878:886	higher frequency	871:886	higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002)	871:1034	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	2	31	theme	SIBO	305:308	arg1	risk					297:300	increased risk	287:300	increased risk of SIBO and related complications	287:334	Obese patients are at increased risk of SIBO and related complications.
36197573	11	32	theme	specific	1503:1510	arg1	symptom					1512:1518	specific symptom	1503:1518	specific symptom	1503:1518	The results of our study conclude that diagnosis cannot be set based on specific symptom and SIBO is related to reduced response to the application of LMWH.
36197573	1	33	theme	excessive	189:197	arg1	overgrowth					154:163	BACKGROUND Small intestinal bacterial overgrowth	116:163	BACKGROUND Small intestinal bacterial overgrowth (SIBO)	116:170	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	33	theme	excessive	189:197	arg1	growth					199:204	an excessive growth	186:204	an excessive growth	186:204	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	4	34	theme	hydrogen	535:542	arg1	test					551:554	a hydrogen breath test	533:554	a hydrogen breath test with glucose substrate (25 g/200 ml of water)	533:600	METHODS Patients underwent a hydrogen breath test with glucose substrate (25 g/200 ml of water).
36197573	10	35	dep	high	1419:1422	arg1	high					1419:1422	high	1419:1422	high	1419:1422	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	10	35	dep	high	1419:1422	arg1	%					1427:1427	43%	1425:1427	43%	1425:1427	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	10	35	dep	high	1419:1422	arg1	incidence					1345:1353	The incidence	1341:1353	The incidence of SIBO after bariatric surgery bypass procedures	1341:1403	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	0	36	theme	Bacterial	17:25	arg1	Overgrowth					27:36	Small Intestinal Bacterial Overgrowth	0:36	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric	0:106	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	10	37	dep	surgery	1379:1385	arg1	procedures					1394:1403	procedures	1394:1403	bariatric surgery bypass procedures	1369:1403	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	10	37	dep	surgery	1379:1385	arg1	bypass					1387:1392	bypass	1387:1392	bypass	1387:1392	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	6	38	theme	NAFLD	725:729	arg1	Score					740:744	the NAFLD Activity Score	721:744	the NAFLD Activity Score	721:744	In 45 patients, the NAFLD Activity Score was evaluated in liver biopsies.
36197573	8	39	from	Score	1057:1061	arg1	patients					1071:1078	SIBO patients	1066:1078	SIBO patients	1066:1078	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	0	40	dep	Gastric	65:71	arg1	Bypass					73:78	Bypass	73:78	Bypass	73:78	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	7	41	theme	breath	795:800	arg1	test					802:805	Glucose breath test	787:805	Glucose breath test	787:805	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	3	42	theme	SIBO	395:398	arg1	incidence					382:390	the incidence	378:390	the incidence of SIBO	378:398	The purpose of this study is to evaluate the incidence of SIBO after bariatric bypass procedures, connection between SIBO, symptoms, comorbidities, and liver pathology.
36197573	7	43	theme	bowel	983:987	arg1	syndrome					989:996	irritable bowel syndrome	973:996	irritable bowel syndrome (p = 0.018)	973:1008	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	43	theme	bowel	983:987	arg1	p = 0.018					999:1007	p = 0.018	999:1007	p = 0.018	999:1007	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	1	44	theme	changed	213:219	arg1	overgrowth					154:163	BACKGROUND Small intestinal bacterial overgrowth	116:163	BACKGROUND Small intestinal bacterial overgrowth (SIBO)	116:170	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	44	theme	changed	213:219	arg1	composition					221:231	changed composition	213:231	changed composition of bacteria	213:243	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	12	45	theme	complications	1761:1773	arg1	development					1742:1752	the development	1738:1752	the development of its complications	1738:1773	Mandatory SIBO screening and appropriate treatment would affect the clinical outcome of the underlying disease, improve it significantly, and prevent the development of its complications.
36197573	4	46	theme	glucose	561:567	arg1	substrate					569:577	glucose substrate	561:577	glucose substrate (25 g/200 ml of water)	561:600	METHODS Patients underwent a hydrogen breath test with glucose substrate (25 g/200 ml of water).
36197573	4	47	dep	substrate	569:577	arg1	25 g/200 ml					580:590	25 g/200 ml	580:590	25 g/200 ml of water	580:599	METHODS Patients underwent a hydrogen breath test with glucose substrate (25 g/200 ml of water).
36197573	7	48	theme	scleroderma	948:958	arg1	frequency					878:886	higher frequency	871:886	higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002)	871:1034	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	8	49	theme	non-SIBO	1110:1117	arg1	n = 27					1129:1134	n = 27	1129:1134	n = 27	1129:1134	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	8	49	theme	non-SIBO	1110:1117	arg1	patients					1119:1126	non-SIBO patients	1110:1126	non-SIBO patients (n = 27)	1110:1135	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	4	50	theme	METHODS	506:512	arg1	Patients					514:521	METHODS Patients	506:521	METHODS Patients	506:521	METHODS Patients underwent a hydrogen breath test with glucose substrate (25 g/200 ml of water).
36197573	7	51	theme	Glucose	787:793	arg1	test					802:805	Glucose breath test	787:805	Glucose breath test	787:805	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	3	52	dep	procedures	423:432	arg1	bypass					416:421	bypass	416:421	bypass	416:421	The purpose of this study is to evaluate the incidence of SIBO after bariatric bypass procedures, connection between SIBO, symptoms, comorbidities, and liver pathology.
36197573	6	53	theme	Activity	731:738	arg1	Score					740:744	the NAFLD Activity Score	721:744	the NAFLD Activity Score	721:744	In 45 patients, the NAFLD Activity Score was evaluated in liver biopsies.
36197573	1	54	theme	Small	127:131	arg1	SIBO					166:169	SIBO	166:169	SIBO	166:169	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	54	theme	Small	127:131	arg1	overgrowth					154:163	BACKGROUND Small intestinal bacterial overgrowth	116:163	BACKGROUND Small intestinal bacterial overgrowth (SIBO)	116:170	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	54	theme	Small	127:131	arg1	growth					199:204	an excessive growth	186:204	an excessive growth	186:204	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	54	theme	Small	127:131	arg1	composition					221:231	changed composition	213:231	changed composition of bacteria	213:243	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	7	55	theme	syndrome	989:996	arg1	frequency					878:886	higher frequency	871:886	higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002)	871:1034	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	9	56	theme	range	1251:1255	arg1	4 h					1271:1273	range value anti-Xa 4 h	1251:1273	range value anti-Xa 4 h after subtherapeutic dose application	1251:1311	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	7	57	theme	irritable	973:981	arg1	syndrome					989:996	irritable bowel syndrome	973:996	irritable bowel syndrome (p = 0.018)	973:1008	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	57	theme	irritable	973:981	arg1	p = 0.018					999:1007	p = 0.018	999:1007	p = 0.018	999:1007	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	58	from	positive	811:818	arg1	24/56					823:827	24/56	823:827	24/56	823:827	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	58	from	positive	811:818	arg1	%					832:832	43%	830:832	43%	830:832	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	58	from	positive	811:818	arg1	patients					838:845	patients	838:845	patients	838:845	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	1	59	from	growth	199:204	arg1	bowel					258:262	the small bowel	248:262	the small bowel	248:262	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	60	theme	intestinal	133:142	arg1	SIBO					166:169	SIBO	166:169	SIBO	166:169	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	60	theme	intestinal	133:142	arg1	overgrowth					154:163	BACKGROUND Small intestinal bacterial overgrowth	116:163	BACKGROUND Small intestinal bacterial overgrowth (SIBO)	116:170	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	60	theme	intestinal	133:142	arg1	growth					199:204	an excessive growth	186:204	an excessive growth	186:204	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	60	theme	intestinal	133:142	arg1	composition					221:231	changed composition	213:231	changed composition of bacteria	213:243	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	11	61	theme	study	1450:1454	arg1	results					1435:1441	The results	1431:1441	The results of our study	1431:1454	The results of our study conclude that diagnosis cannot be set based on specific symptom and SIBO is related to reduced response to the application of LMWH.
36197573	9	62	theme	value	1257:1261	arg1	4 h					1271:1273	range value anti-Xa 4 h	1251:1273	range value anti-Xa 4 h after subtherapeutic dose application	1251:1311	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	2	63	theme	Obese	265:269	arg1	patients					271:278	Obese patients	265:278	Obese patients	265:278	Obese patients are at increased risk of SIBO and related complications.
36197573	12	64	theme	SIBO	1598:1601	arg1	screening					1603:1611	Mandatory SIBO screening	1588:1611	Mandatory SIBO screening	1588:1611	Mandatory SIBO screening and appropriate treatment would affect the clinical outcome of the underlying disease, improve it significantly, and prevent the development of its complications.
36197573	1	65	theme	bacterial	144:152	arg1	SIBO					166:169	SIBO	166:169	SIBO	166:169	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	65	theme	bacterial	144:152	arg1	overgrowth					154:163	BACKGROUND Small intestinal bacterial overgrowth	116:163	BACKGROUND Small intestinal bacterial overgrowth (SIBO)	116:170	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	65	theme	bacterial	144:152	arg1	growth					199:204	an excessive growth	186:204	an excessive growth	186:204	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	65	theme	bacterial	144:152	arg1	composition					221:231	changed composition	213:231	changed composition of bacteria	213:243	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	7	66	theme	lactose	915:921	arg1	p = 0.047					936:944	p = 0.047	936:944	p = 0.047	936:944	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	7	66	theme	lactose	915:921	arg1	intolerance					923:933	lactose intolerance	915:933	lactose intolerance (p = 0.047)	915:945	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	4	67	theme	water	595:599	arg1	25 g/200 ml					580:590	25 g/200 ml	580:590	25 g/200 ml of water	580:599	METHODS Patients underwent a hydrogen breath test with glucose substrate (25 g/200 ml of water).
36197573	9	68	theme	SIBO-positive	1141:1153	arg1	cohort					1155:1160	SIBO-positive cohort	1141:1160	SIBO-positive cohort of patients	1141:1172	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	7	69	from	24/56	823:827	arg1	positive					811:818	positive	811:818	positive	811:818	RESULTS Glucose breath test was positive in 24/56 (43%) of patients and was associated with higher frequency of defecation (p = 0.022), lactose intolerance (p = 0.047), scleroderma (p = 0.042), irritable bowel syndrome (p = 0.018), and diabetes (p = 0.002).
36197573	2	70	theme	related	314:320	arg1	complications					322:334	related complications	314:334	related complications	314:334	Obese patients are at increased risk of SIBO and related complications.
36197573	12	71	theme	Mandatory	1588:1596	arg1	screening					1603:1611	Mandatory SIBO screening	1588:1611	Mandatory SIBO screening	1588:1611	Mandatory SIBO screening and appropriate treatment would affect the clinical outcome of the underlying disease, improve it significantly, and prevent the development of its complications.
36197573	1	72	theme	bacteria	236:243	arg1	overgrowth					154:163	BACKGROUND Small intestinal bacterial overgrowth	116:163	BACKGROUND Small intestinal bacterial overgrowth (SIBO)	116:170	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	1	72	theme	bacteria	236:243	arg1	composition					221:231	changed composition	213:231	changed composition of bacteria	213:243	BACKGROUND Small intestinal bacterial overgrowth (SIBO) is defined as an excessive growth and/or changed composition of bacteria in the small bowel.
36197573	8	73	theme	NAFLD	1042:1046	arg1	Score					1057:1061	Mean NAFLD Activity Score	1037:1061	Mean NAFLD Activity Score in SIBO patients (n = 18)	1037:1087	Mean NAFLD Activity Score in SIBO patients (n = 18) was 3.33 and 3.00 in non-SIBO patients (n = 27).
36197573	11	74	theme	LMWH	1582:1585	arg1	application					1567:1577	the application	1563:1577	the application of LMWH	1563:1585	The results of our study conclude that diagnosis cannot be set based on specific symptom and SIBO is related to reduced response to the application of LMWH.
36197573	12	75	theme	clinical	1656:1663	arg1	outcome					1665:1671	the clinical outcome	1652:1671	the clinical outcome of the underlying disease	1652:1697	Mandatory SIBO screening and appropriate treatment would affect the clinical outcome of the underlying disease, improve it significantly, and prevent the development of its complications.
36197573	12	76	theme	appropriate	1617:1627	arg1	treatment					1629:1637	appropriate treatment	1617:1637	appropriate treatment	1617:1637	Mandatory SIBO screening and appropriate treatment would affect the clinical outcome of the underlying disease, improve it significantly, and prevent the development of its complications.
36197573	10	77	dep	CONCLUSIONS	1329:1339	arg1	high					1419:1422	high	1419:1422	high	1419:1422	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	10	77	dep	CONCLUSIONS	1329:1339	arg1	%					1427:1427	43%	1425:1427	43%	1425:1427	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	10	77	dep	CONCLUSIONS	1329:1339	arg1	incidence					1345:1353	The incidence	1341:1353	The incidence of SIBO after bariatric surgery bypass procedures	1341:1403	CONCLUSIONS The incidence of SIBO after bariatric surgery bypass procedures is alarmingly high (43%).
36197573	0	78	theme	Gastric	65:71	arg1	Roux-en-Y					55:63	Roux-en-Y	55:63	Roux-en-Y Gastric Bypass and One-Anastomosis Gastric	55:106	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	0	79	from	Overgrowth	27:36	arg1	Patients					41:48	Patients	41:48	Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric	41:106	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	9	80	theme	subtherapeutic	1281:1294	arg1	application					1301:1311	subtherapeutic dose application	1281:1311	subtherapeutic dose application	1281:1311	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	0	81	with	Patients	41:48	arg1	Roux-en-Y					55:63	Roux-en-Y	55:63	Roux-en-Y Gastric Bypass and One-Anastomosis Gastric	55:106	Small Intestinal Bacterial Overgrowth in Patients with Roux-en-Y Gastric Bypass and One-Anastomosis Gastric Bypass.
36197573	9	82	theme	anti-Xa	1263:1269	arg1	4 h					1271:1273	range value anti-Xa 4 h	1251:1273	range value anti-Xa 4 h after subtherapeutic dose application	1251:1311	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	9	83	from	trend	1201:1205	arg1	difference					1210:1219	difference	1210:1219	difference between NAS	1210:1231	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	9	83	from	trend	1201:1205	arg1	difference					1237:1246	difference	1237:1246	difference	1237:1246	In SIBO-positive cohort of patients, a statistically important trend in difference between NAS and difference to range value anti-Xa 4 h after subtherapeutic dose application was calculated.
36197573	3	84	theme	study	357:361	arg1	purpose					341:347	The purpose	337:347	The purpose of this study	337:361	The purpose of this study is to evaluate the incidence of SIBO after bariatric bypass procedures, connection between SIBO, symptoms, comorbidities, and liver pathology.
36290991	5	0	from	loss	1115:1118	arg1	performance					1123:1133	performance	1123:1133	performance	1123:1133	A single cortisol sensor patch could be reused 15 times over a 30-day period with no loss in performance, attesting to excellent reusability.
36290991	6	1	from	levels	1262:1267	arg1	sweat					1278:1282	human sweat	1272:1282	human sweat	1272:1282	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	1	2	from	sensor	183:188	arg1	sweat					222:226	human sweat	216:226	human sweat	216:226	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	1	3	theme	MIP	162:164	arg1	sensor					183:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor	105:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat	105:226	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	2	4	from	assembly	314:321	arg1	substrate					358:366	a flexible cotton textile substrate	332:366	a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	332:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	4	5	theme	physiological	993:1005	arg1	levels					1007:1012	the typical physiological levels	981:1012	the typical physiological levels in human sweat	981:1027	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	4	6	theme	detection	934:942	arg1	limit					925:929	an excellent limit	912:929	an excellent limit	912:929	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	3	7	theme	cortisol	690:697	arg1	sensor					699:704	The cortisol sensor	686:704	The cortisol sensor	686:704	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	6	8	theme	human	1272:1276	arg1	sweat					1278:1282	human sweat	1272:1282	human sweat	1272:1282	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	2	9	theme	textile	350:356	arg1	substrate					358:366	a flexible cotton textile substrate	332:366	a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	332:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	3	10	theme	%	824:824	arg1	deviation					801:809	an average relative standard deviation	772:809	an average relative standard deviation (%RSD) of 6.4% across the dynamic range	772:849	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	3	10	theme	%	824:824	arg1	RSD					813:815	%RSD	812:815	%RSD	812:815	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	3	11	theme	%	812:812	arg1	deviation					801:809	an average relative standard deviation	772:809	an average relative standard deviation (%RSD) of 6.4% across the dynamic range	772:849	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	3	11	theme	%	812:812	arg1	RSD					813:815	%RSD	812:815	%RSD	812:815	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	6	12	from	quantification	1235:1248	arg1	sweat					1278:1282	human sweat	1272:1282	human sweat	1272:1282	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	2	13	theme	layer-by-layer	299:312	arg1	LbL					324:326	LbL	324:326	LbL	324:326	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	13	theme	layer-by-layer	299:312	arg1	assembly					314:321	layer-by-layer assembly	299:321	layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	299:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	4	14	theme	typical	985:991	arg1	levels					1007:1012	the typical physiological levels	981:1012	the typical physiological levels in human sweat	981:1027	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	3	15	theme	standard	792:799	arg1	deviation					801:809	an average relative standard deviation	772:809	an average relative standard deviation (%RSD) of 6.4% across the dynamic range	772:849	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	3	15	theme	standard	792:799	arg1	RSD					813:815	%RSD	812:815	%RSD	812:815	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	6	16	theme	sensor	1185:1190	arg1	patch					1192:1196	The cortisol sensor patch	1172:1196	The cortisol sensor patch	1172:1196	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	4	17	from	levels	1007:1012	arg1	sweat					1023:1027	human sweat	1017:1027	human sweat	1017:1027	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	2	18	theme	cortisol-imprinted	521:538	arg1	dimethacrylate					585:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	18	theme	cortisol-imprinted	521:538	arg1	poly					540:543	a selective cortisol-imprinted poly	509:543	a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	509:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	6	19	theme	cortisol	1176:1183	arg1	patch					1192:1196	The cortisol sensor patch	1172:1196	The cortisol sensor patch	1172:1196	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	3	20	theme	dynamic	837:843	arg1	range					845:849	the dynamic range	833:849	the dynamic range	833:849	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	3	21	theme	excellent	863:871	arg1	precision					873:881	excellent precision	863:881	excellent precision	863:881	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	1	22	theme	electrochemical	167:181	arg1	sensor					183:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor	105:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat	105:226	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	2	23	theme	selective	511:519	arg1	dimethacrylate					585:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	23	theme	selective	511:519	arg1	poly					540:543	a selective cortisol-imprinted poly	509:543	a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	509:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	24	theme	nanocrystal	430:440	arg1	suspension					462:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	4	25	theme	ng/mL	958:962	arg1	LOD					945:947	LOD	945:947	LOD	945:947	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	4	25	theme	ng/mL	958:962	arg1	detection					934:942	detection	934:942	detection (LOD) of 8.00 ng/mL	934:962	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	2	26	theme	nanoporous	393:402	arg1	suspension					462:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	0	27	theme	Wearable	2:9	arg1	Sensor					65:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor	0:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.	0:103	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.
36290991	5	28	theme	cortisol	1039:1046	arg1	patch					1055:1059	A single cortisol sensor patch	1030:1059	A single cortisol sensor patch	1030:1059	A single cortisol sensor patch could be reused 15 times over a 30-day period with no loss in performance, attesting to excellent reusability.
36290991	2	29	theme	conductive	382:391	arg1	suspension					462:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	4	30	theme	cortisol	888:895	arg1	sensor					897:902	The cortisol sensor	884:902	The cortisol sensor	884:902	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	2	31	theme	gold	637:640	arg1	nanoparticles					642:654	gold nanoparticles	637:654	gold nanoparticles (AuNPs)	637:662	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	31	theme	gold	637:640	arg1	AuNPs					657:661	AuNPs	657:661	AuNPs	657:661	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	1	32	theme	cortisol	194:201	arg1	detection					203:211	cortisol detection	194:211	cortisol detection	194:211	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	2	33	theme	composite	452:460	arg1	suspension					462:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	34	theme	wearable	255:262	arg1	sensor					273:278	The wearable cortisol sensor	251:278	The wearable cortisol sensor	251:278	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	6	35	theme	levels	1262:1267	arg1	quantification					1235:1248	quantification	1235:1248	quantification of cortisol levels in human sweat	1235:1282	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	6	36	from	sweat	1278:1282	arg1	quantification					1235:1248	quantification	1235:1248	quantification of cortisol levels in human sweat	1235:1282	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	2	37	theme	CNT/CNC	443:449	arg1	suspension					462:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	3	38	dep	rapidly	706:712	arg1	min					721:723	&lt;2 min	715:723	&lt;2 min	715:723	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	6	39	theme	cortisol	1253:1260	arg1	levels					1262:1267	cortisol levels	1253:1267	cortisol levels in human sweat	1253:1282	The cortisol sensor patch was successfully verified for use in quantification of cortisol levels in human sweat.
36290991	1	40	theme	wearable	107:114	arg1	sensor					183:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor	105:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat	105:226	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	0	41	theme	Imprinted	39:47	arg1	Sensor					65:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor	0:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.	0:103	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.
36290991	2	42	theme	glycol	578:583	arg1	dimethacrylate					585:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	42	theme	glycol	578:583	arg1	poly					540:543	a selective cortisol-imprinted poly	509:543	a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	509:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	0	43	from	Detection	85:93	arg1	Sweat					98:102	Sweat	98:102	Sweat	98:102	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.
36290991	1	44	theme	human	216:220	arg1	sweat					222:226	human sweat	216:226	human sweat	216:226	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	2	45	theme	carbon	404:409	arg1	suspension					462:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	3	46	theme	cortisol	757:764	arg1	ng/mL					748:752	9.8-49.5 ng/mL	739:752	9.8-49.5 ng/mL of cortisol	739:764	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	0	47	theme	Polyacrylate	26:37	arg1	Sensor					65:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor	0:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.	0:103	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.
36290991	2	48	theme	glycidylmethacrylate-co-ethylene	545:576	arg1	dimethacrylate					585:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	glycidylmethacrylate-co-ethylene glycol dimethacrylate	545:598	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	48	theme	glycidylmethacrylate-co-ethylene	545:576	arg1	poly					540:543	a selective cortisol-imprinted poly	509:543	a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	509:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	49	theme	nanotube/cellulose	411:428	arg1	suspension					462:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension	380:471	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	5	50	theme	30-day	1093:1098	arg1	period					1100:1105	a 30-day period	1091:1105	a 30-day period	1091:1105	A single cortisol sensor patch could be reused 15 times over a 30-day period with no loss in performance, attesting to excellent reusability.
36290991	3	51	theme	&lt	715:717	arg1	min					721:723	&lt;2 min	715:723	&lt;2 min	715:723	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	2	52	theme	cotton	343:348	arg1	substrate					358:366	a flexible cotton textile substrate	332:366	a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	332:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	0	53	theme	Electrochemical	49:63	arg1	Sensor					65:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor	0:70	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.	0:103	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.
36290991	2	54	theme	conductive	474:483	arg1	polyaniline					485:495	conductive polyaniline	474:495	conductive polyaniline (PANI)	474:502	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	54	theme	conductive	474:483	arg1	PANI					498:501	PANI	498:501	PANI	498:501	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	55	theme	flexible	334:341	arg1	substrate					358:366	a flexible cotton textile substrate	332:366	a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold	332:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	4	56	theme	excellent	915:923	arg1	limit					925:929	an excellent limit	912:929	an excellent limit	912:929	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	3	57	theme	average	775:781	arg1	deviation					801:809	an average relative standard deviation	772:809	an average relative standard deviation (%RSD) of 6.4% across the dynamic range	772:849	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	3	57	theme	average	775:781	arg1	RSD					813:815	%RSD	812:815	%RSD	812:815	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	2	58	theme	cortisol	264:271	arg1	sensor					273:278	The wearable cortisol sensor	251:278	The wearable cortisol sensor	251:278	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	59	dep	poly	540:543	arg1	decorated					622:630	decorated	622:630	decorated with gold nanoparticles (AuNPs)	622:662	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	59	dep	poly	540:543	arg1	poly					602:605	poly	602:605	poly(GMA-co-EGDMA)	602:619	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	59	dep	poly	540:543	arg1	plated					668:673	plated	668:673	plated with gold	668:683	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	2	59	dep	poly	540:543	arg1	GMA-co-EGDMA					607:618	GMA-co-EGDMA	607:618	GMA-co-EGDMA	607:618	The wearable cortisol sensor was fabricated via layer-by-layer assembly (LbL) on a flexible cotton textile substrate coated with a conductive nanoporous carbon nanotube/cellulose nanocrystal (CNT/CNC) composite suspension, conductive polyaniline (PANI), and a selective cortisol-imprinted poly(glycidylmethacrylate-co-ethylene glycol dimethacrylate) (poly(GMA-co-EGDMA)) decorated with gold nanoparticles (AuNPs), or plated with gold.
36290991	1	60	theme	imprinted	143:151	arg1	sensor					183:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor	105:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat	105:226	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	3	61	theme	relative	783:790	arg1	deviation					801:809	an average relative standard deviation	772:809	an average relative standard deviation (%RSD) of 6.4% across the dynamic range	772:849	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	3	61	theme	relative	783:790	arg1	RSD					813:815	%RSD	812:815	%RSD	812:815	The cortisol sensor rapidly (&lt;2 min) responded to 9.8-49.5 ng/mL of cortisol, with an average relative standard deviation (%RSD) of 6.4% across the dynamic range, indicating excellent precision.
36290991	0	62	theme	Cortisol	76:83	arg1	Detection					85:93	Cortisol Detection	76:93	Cortisol Detection in Sweat	76:102	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.
36290991	0	63	dep	Wearable	2:9	arg1	Textile-Based					12:24	Textile-Based	12:24	Textile-Based	12:24	A Wearable, Textile-Based Polyacrylate Imprinted Electrochemical Sensor for Cortisol Detection in Sweat.
36290991	1	64	theme	polymer	153:159	arg1	sensor					183:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor	105:188	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat	105:226	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	4	65	theme	human	1017:1021	arg1	sweat					1023:1027	human sweat	1017:1027	human sweat	1017:1027	The cortisol sensor yielded an excellent limit of detection (LOD) of 8.00 ng/mL, which is within the typical physiological levels in human sweat.
36290991	5	66	theme	sensor	1048:1053	arg1	patch					1055:1059	A single cortisol sensor patch	1030:1059	A single cortisol sensor patch	1030:1059	A single cortisol sensor patch could be reused 15 times over a 30-day period with no loss in performance, attesting to excellent reusability.
36290991	5	67	theme	single	1032:1037	arg1	patch					1055:1059	A single cortisol sensor patch	1030:1059	A single cortisol sensor patch	1030:1059	A single cortisol sensor patch could be reused 15 times over a 30-day period with no loss in performance, attesting to excellent reusability.
36290991	1	68	dep	wearable	107:114	arg1	textile-based					117:129	textile-based	117:129	textile-based	117:129	A wearable, textile-based molecularly imprinted polymer (MIP) electrochemical sensor for cortisol detection in human sweat has been demonstrated.
36290991	5	69	theme	excellent	1149:1157	arg1	reusability					1159:1169	excellent reusability	1149:1169	excellent reusability	1149:1169	A single cortisol sensor patch could be reused 15 times over a 30-day period with no loss in performance, attesting to excellent reusability.
36329527	11	0	theme	IGMs	2063:2066	arg1	production					2035:2044	scalable and controllable production	2009:2044	scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering	2009:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	9	1	theme	PRP-loaded	1628:1637	arg1	DPC/EPC					1639:1645	a PRP-loaded DPC/EPC	1626:1645	a PRP-loaded DPC/EPC	1626:1645	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	6	2	theme	long	1213:1216	arg1	phase					1230:1234	a relatively long hair growth phase	1200:1234	a relatively long hair growth phase	1200:1234	Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase.
36329527	3	3	theme	GelMA/chitosan-microcarriers	735:762	arg1	IGMs					765:768	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs)	688:769	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs	688:829	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	8	4	dep	control	1490:1496	arg1	group					1507:1511	group (p < 0.05)	1507:1522	group (p < 0.05)	1507:1522	Importantly, PRP-loaded IGMs presented a higher hair inducibility of DPCs in vitro compared to the control and IGMs group (p < 0.05).
36329527	3	5	theme	interpenetrating	710:725	arg1	IGMs					765:768	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs)	688:769	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs	688:829	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	11	6	theme	DECHS	2072:2076	arg1	production					2035:2044	scalable and controllable production	2009:2044	scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering	2009:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	11	7	theme	ECM-mimicking	2049:2061	arg1	IGMs					2063:2066	ECM-mimicking IGMs	2049:2066	ECM-mimicking IGMs	2049:2066	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	11	8	theme	new	2216:2218	arg1	strategy					2220:2227	a potential new strategy	2204:2227	a potential new strategy for HF tissue engineering	2204:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	5	9	with	production	980:989	arg1	diameters					1025:1033	distinct and precise diameters	1004:1033	distinct and precise diameters	1004:1033	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	11	10	theme	high-throughput	1966:1980	arg1	microfluidics					1982:1994	high-throughput microfluidics	1966:1994	high-throughput microfluidics	1966:1994	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	1	11	theme	follicles	210:218	arg1	engineering					190:200	BACKGROUND Tissue engineering	172:200	BACKGROUND Tissue engineering of hair follicles (HFs)	172:224	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	6	12	theme	factor	1171:1176	arg1	release					1178:1184	sustained growth factor release	1154:1184	sustained growth factor release	1154:1184	Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase.
36329527	5	13	theme	flow	1087:1090	arg1	ratio					1097:1101	the oil and aqueous phase flow rate ratio	1061:1101	the oil and aqueous phase flow rate ratio	1061:1101	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	3	14	dep	methacryloyl	604:615	arg1	hydrogels					638:646	hydrogels	638:646	hydrogels	638:646	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	4	15	theme	high	960:963	arg1	porosity					965:972	high porosity	960:972	high porosity	960:972	RESULTS The ECM-mimicking hydrogels used for IGMs exhibited surface nano-topography and high porosity.
36329527	11	16	theme	tissue	2236:2241	arg1	engineering					2243:2253	HF tissue engineering	2233:2253	HF tissue engineering	2233:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	0	17	theme	follicle	143:150	arg1	engineering					159:169	hair follicle tissue engineering	138:169	hair follicle tissue engineering	138:169	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	11	18	theme	controllable	2022:2033	arg1	production					2035:2044	scalable and controllable production	2009:2044	scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering	2009:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	8	19	theme	DPCs	1460:1463	arg1	inducibility					1444:1455	a higher hair inducibility	1430:1455	a higher hair inducibility of DPCs	1430:1463	Importantly, PRP-loaded IGMs presented a higher hair inducibility of DPCs in vitro compared to the control and IGMs group (p < 0.05).
36329527	7	20	theme	control	1375:1381	arg1	group					1383:1387	control group	1375:1387	control group	1375:1387	DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group).
36329527	3	21	theme	platelet-rich	783:795	arg1	plasma					797:802	platelet-rich plasma	783:802	platelet-rich plasma (PRP)	783:808	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	3	21	theme	platelet-rich	783:795	arg1	PRP					805:807	PRP	805:807	PRP	805:807	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	5	22	theme	oil	1065:1067	arg1	ratio					1097:1101	the oil and aqueous phase flow rate ratio	1061:1101	the oil and aqueous phase flow rate ratio	1061:1101	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	11	23	theme	scalable	2009:2016	arg1	production					2035:2044	scalable and controllable production	2009:2044	scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering	2009:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	7	24	theme	greater	1362:1368	arg1	1.2-fold					1353:1360	1.2-fold	1353:1360	1.2-fold greater than control group	1353:1387	DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group).
36329527	3	25	used	utilized	587:594	arg2	we					584:585	we	584:585	we	584:585	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	5	26	theme	aqueous	1073:1079	arg1	ratio					1097:1101	the oil and aqueous phase flow rate ratio	1061:1101	the oil and aqueous phase flow rate ratio	1061:1101	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	3	27	theme	high-throughput	837:851	arg1	chip					866:869	a high-throughput microfluidic chip	835:869	a high-throughput microfluidic chip	835:869	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	11	28	theme	hair	2167:2170	arg1	regeneration					2172:2183	hair regeneration	2167:2183	hair regeneration	2167:2183	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	6	29	theme	growth	1223:1228	arg1	phase					1230:1234	a relatively long hair growth phase	1200:1234	a relatively long hair growth phase	1200:1234	Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase.
36329527	7	30	theme	PRP-loaded	1252:1261	arg1	IGMs					1263:1266	PRP-loaded IGMs	1252:1266	PRP-loaded IGMs	1252:1266	DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group).
36329527	3	31	dep	IGMs	765:768	arg1	seeded					814:819	seeded	814:819	seeded with DPCs	814:829	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	3	31	dep	IGMs	765:768	arg1	loaded					771:776	loaded	771:776	loaded with platelet-rich plasma (PRP)	771:808	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	0	32	theme	PRP	79:81	arg1	/cell-laden					83:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	1	33	theme	Tissue	183:188	arg1	engineering					190:200	BACKGROUND Tissue engineering	172:200	BACKGROUND Tissue engineering of hair follicles (HFs)	172:224	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	2	34	theme	ECM	523:525	arg1	substrates					538:547	extracellular matrix (ECM)-mimicking substrates	501:547	extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	501:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	0	35	theme	/cell-laden	83:93	arg1	production					29:38	Scalable and high-throughput production	0:38	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden	0:93	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	5	36	theme	IGMs	994:997	arg1	production					980:989	Mass production	975:989	Mass production of IGMs with distinct and precise diameters	975:1033	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	2	37	theme	matrix	515:520	arg1	substrates					538:547	extracellular matrix (ECM)-mimicking substrates	501:547	extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	501:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	3	38	from	IGMs	765:768	arg1	chip					866:869	a high-throughput microfluidic chip	835:869	a high-throughput microfluidic chip	835:869	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	0	39	theme	composite	117:125	arg1	system					127:132	composite system	117:132	composite system for hair follicle tissue engineering	117:169	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	5	40	theme	distinct	1004:1011	arg1	diameters					1025:1033	distinct and precise diameters	1004:1033	distinct and precise diameters	1004:1033	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	2	41	theme	associated	425:434	arg1	process					472:478	the associated inefficient and tedious preparation process	421:478	the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	421:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	2	42	theme	proliferation	374:386	arg1	process					472:478	the associated inefficient and tedious preparation process	421:478	the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	421:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	2	42	theme	proliferation	374:386	arg1	weakening					320:328	weakening	320:328	weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility	320:407	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	0	43	theme	Scalable	0:7	arg1	production					29:38	Scalable and high-throughput production	0:38	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden	0:93	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	0	44	theme	high-throughput	13:27	arg1	production					29:38	Scalable and high-throughput production	0:38	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden	0:93	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	2	45	theme	preparation	460:470	arg1	process					472:478	the associated inefficient and tedious preparation process	421:478	the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	421:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	2	46	theme	inducibility	396:407	arg1	process					472:478	the associated inefficient and tedious preparation process	421:478	the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	421:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	2	46	theme	inducibility	396:407	arg1	weakening					320:328	weakening	320:328	weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility	320:407	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	11	47	theme	DPC	2147:2149	arg1	bioactivity					2151:2161	DPC bioactivity	2147:2161	DPC bioactivity	2147:2161	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	9	48	theme	cell	1595:1598	arg1	GelMA					1612:1616	epidermal cell (EPC)-laden GelMA	1585:1616	epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice	1585:1749	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	0	49	theme	plasma	71:76	arg1	/cell-laden					83:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	2	50	theme	cell	352:355	arg1	viability					363:371	the dermal papilla cell (DPC) viability	333:371	the dermal papilla cell (DPC) viability	333:371	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	0	51	theme	injectable	46:55	arg1	/cell-laden					83:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	9	52	theme	EPC	1601:1603	arg1	GelMA					1612:1616	epidermal cell (EPC)-laden GelMA	1585:1616	epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice	1585:1749	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	2	53	theme	dermal	337:342	arg1	papilla					344:350	the dermal papilla	333:350	the dermal papilla cell (DPC) viability	333:371	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	1	54	theme	enormous	230:237	arg1	potential					239:247	enormous potential	230:247	enormous potential for hair loss treatment	230:271	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	5	55	theme	precise	1017:1023	arg1	diameters					1025:1033	distinct and precise diameters	1004:1033	distinct and precise diameters	1004:1033	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	6	56	theme	appropriate	1129:1139	arg1	swelling					1141:1148	appropriate swelling	1129:1148	appropriate swelling	1129:1148	Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase.
36329527	10	57	dep	vessels	1832:1838	arg1	~ 12					1841:1844	~ 12	1841:1844	~ 12	1841:1844	The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each).
36329527	8	58	theme	hair	1439:1442	arg1	inducibility					1444:1455	a higher hair inducibility	1430:1455	a higher hair inducibility of DPCs	1430:1463	Importantly, PRP-loaded IGMs presented a higher hair inducibility of DPCs in vitro compared to the control and IGMs group (p < 0.05).
36329527	2	59	theme	viability	363:371	arg1	process					472:478	the associated inefficient and tedious preparation process	421:478	the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	421:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	2	59	theme	viability	363:371	arg1	weakening					320:328	weakening	320:328	weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility	320:407	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	3	60	from	scalable	659:666	arg1	chip					866:869	a high-throughput microfluidic chip	835:869	a high-throughput microfluidic chip	835:869	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	3	61	theme	diameter-controllable	688:708	arg1	IGMs					765:768	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs)	688:769	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs	688:829	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	10	62	theme	other	1865:1869	arg1	groups					1871:1876	the other groups	1861:1876	the other groups (p < 0.05 for each)	1861:1896	The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each).
36329527	2	63	theme	DPC	358:360	arg1	viability					363:371	the dermal papilla cell (DPC) viability	333:371	the dermal papilla cell (DPC) viability	333:371	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	1	64	theme	loss	258:261	arg1	treatment					263:271	hair loss treatment	253:271	hair loss treatment	253:271	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	4	65	theme	ECM-mimicking	884:896	arg1	hydrogels					898:906	The ECM-mimicking hydrogels	880:906	The ECM-mimicking hydrogels used for IGMs	880:920	RESULTS The ECM-mimicking hydrogels used for IGMs exhibited surface nano-topography and high porosity.
36329527	2	66	theme	certain	283:289	arg1	challenges					291:300	certain challenges	283:300	certain challenges	283:300	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	4	67	dep	RESULTS	872:878	arg1	exhibited					922:930	exhibited	922:930	exhibited surface nano-topography and high porosity	922:972	RESULTS The ECM-mimicking hydrogels used for IGMs exhibited surface nano-topography and high porosity.
36329527	9	68	theme	hydrogel	1659:1666	arg1	DECHS					1676:1680	DECHS	1676:1680	DECHS	1676:1680	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	9	68	theme	hydrogel	1659:1666	arg1	system					1668:1673	hydrogel system	1659:1673	hydrogel system (DECHS)	1659:1681	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	11	69	theme	potential	2206:2214	arg1	strategy					2220:2227	a potential new strategy	2204:2227	a potential new strategy for HF tissue engineering	2204:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	10	70	dep	HFs	1802:1804	arg1	~ 35					1807:1810	~ 35	1807:1810	~ 35	1807:1810	The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each).
36329527	3	71	theme	network	727:733	arg1	IGMs					765:768	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs)	688:769	diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs	688:829	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	10	72	theme	PRP-loaded	1756:1765	arg1	DECHS					1767:1771	The PRP-loaded DECHS	1752:1771	The PRP-loaded DECHS	1752:1771	The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each).
36329527	1	73	theme	hair	205:208	arg1	HFs					221:223	HFs	221:223	HFs	221:223	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	1	73	theme	hair	205:208	arg1	follicles					210:218	hair follicles	205:218	hair follicles (HFs)	205:224	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	0	74	theme	hair	138:141	arg1	follicle					143:150	hair follicle	138:150	hair follicle tissue engineering	138:169	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	7	75	dep	properties	1341:1350	arg1	1.2-fold					1353:1360	1.2-fold	1353:1360	1.2-fold greater than control group	1353:1387	DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group).
36329527	4	76	theme	surface	932:938	arg1	nano-topography					940:954	surface nano-topography	932:954	surface nano-topography	932:954	RESULTS The ECM-mimicking hydrogels used for IGMs exhibited surface nano-topography and high porosity.
36329527	6	77	theme	growth	1164:1169	arg1	release					1178:1184	sustained growth factor release	1154:1184	sustained growth factor release	1154:1184	Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase.
36329527	11	78	theme	HF	2233:2234	arg1	engineering					2243:2253	HF tissue engineering	2233:2253	HF tissue engineering	2233:2253	CONCLUSION Taken together, these results illustrate that, based on high-throughput microfluidics, we obtained scalable and controllable production of ECM-mimicking IGMs and DECHS, which simulate an effective micro- and macro-environment to promote DPC bioactivity and hair regeneration, thus representing a potential new strategy for HF tissue engineering.
36329527	3	79	from	monodispersed	669:681	arg1	chip					866:869	a high-throughput microfluidic chip	835:869	a high-throughput microfluidic chip	835:869	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	0	80	theme	tissue	152:157	arg1	engineering					159:169	hair follicle tissue engineering	138:169	hair follicle tissue engineering	138:169	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	5	81	theme	rate	1092:1095	arg1	ratio					1097:1101	the oil and aqueous phase flow rate ratio	1061:1101	the oil and aqueous phase flow rate ratio	1061:1101	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	10	82	theme	more	1797:1800	arg1	HFs					1802:1804	significantly more HFs	1783:1804	significantly more HFs (~ 35 per site)	1783:1820	The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each).
36329527	9	83	theme	mice	1746:1749	arg1	hypodermis					1727:1736	the hypodermis	1723:1736	the hypodermis of nude mice	1723:1749	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	5	84	theme	phase	1081:1085	arg1	ratio					1097:1101	the oil and aqueous phase flow rate ratio	1061:1101	the oil and aqueous phase flow rate ratio	1061:1101	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	3	85	theme	gelatin	596:602	arg1	methacryloyl					604:615	gelatin methacryloyl	596:615	gelatin methacryloyl (GelMA)	596:623	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	3	85	theme	gelatin	596:602	arg1	GelMA					618:622	GelMA	618:622	GelMA	618:622	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	3	86	theme	microfluidic	853:864	arg1	chip					866:869	a high-throughput microfluidic chip	835:869	a high-throughput microfluidic chip	835:869	Herein, we utilized gelatin methacryloyl (GelMA) and chitosan hydrogels to prepare scalable, monodispersed, and diameter-controllable interpenetrating network GelMA/chitosan-microcarriers (IGMs) loaded with platelet-rich plasma (PRP) and seeded with DPCs, on a high-throughput microfluidic chip.
36329527	6	87	theme	hair	1218:1221	arg1	phase					1230:1234	a relatively long hair growth phase	1200:1234	a relatively long hair growth phase	1200:1234	Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase.
36329527	1	88	theme	BACKGROUND	172:181	arg1	engineering					190:200	BACKGROUND Tissue engineering	172:200	BACKGROUND Tissue engineering of hair follicles (HFs)	172:224	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	9	89	theme	nude	1741:1744	arg1	mice					1746:1749	nude mice	1741:1749	nude mice	1741:1749	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	9	90	theme	-laden	1605:1610	arg1	GelMA					1612:1616	epidermal cell (EPC)-laden GelMA	1585:1616	epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice	1585:1749	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	9	91	theme	DPC/PRP-laden	1538:1550	arg1	IGMs					1552:1555	DPC/PRP-laden IGMs	1538:1555	DPC/PRP-laden IGMs	1538:1555	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	7	92	theme	proliferative	1327:1339	arg1	properties					1341:1350	proliferative properties	1327:1350	proliferative properties (1.2-fold greater than control group)	1327:1388	DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group).
36329527	2	93	theme	extracellular	501:513	arg1	substrates					538:547	extracellular matrix (ECM)-mimicking substrates	501:547	extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	501:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	2	94	theme	-mimicking	527:536	arg1	substrates					538:547	extracellular matrix (ECM)-mimicking substrates	501:547	extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	501:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	10	95	dep	groups	1871:1876	arg1	p < 0.05					1879:1886	p < 0.05	1879:1886	p < 0.05	1879:1886	The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each).
36329527	5	96	theme	Mass	975:978	arg1	production					980:989	Mass production	975:989	Mass production of IGMs with distinct and precise diameters	975:1033	Mass production of IGMs with distinct and precise diameters was achieved by adjusting the oil and aqueous phase flow rate ratio.
36329527	10	97	theme	novel	1826:1830	arg1	vessels					1832:1838	novel vessels	1826:1838	novel vessels (~ 12 per site)	1826:1854	The PRP-loaded DECHS generated significantly more HFs (~ 35 per site) and novel vessels (~ 12 per site) than the other groups (p < 0.05 for each).
36329527	9	98	theme	epidermal	1585:1593	arg1	GelMA					1612:1616	epidermal cell (EPC)-laden GelMA	1585:1616	epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice	1585:1749	Furthermore, DPC/PRP-laden IGMs were effectively mixed with epidermal cell (EPC)-laden GelMA to form a PRP-loaded DPC/EPC co-cultured hydrogel system (DECHS), which was subcutaneously injected into the hypodermis of nude mice.
36329527	2	99	theme	tedious	452:458	arg1	process					472:478	the associated inefficient and tedious preparation process	421:478	the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	421:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	2	100	theme	HF	393:394	arg1	inducibility					396:407	HF inducibility	393:407	HF inducibility	393:407	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	0	101	theme	platelet-rich	57:69	arg1	/cell-laden					83:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	an injectable platelet-rich plasma (PRP)/cell-laden	43:93	Scalable and high-throughput production of an injectable platelet-rich plasma (PRP)/cell-laden microcarrier/hydrogel composite system for hair follicle tissue engineering.
36329527	6	102	theme	sustained	1154:1162	arg1	release					1178:1184	sustained growth factor release	1154:1184	sustained growth factor release	1154:1184	Moreover, IGMs exhibited appropriate swelling and sustained growth factor release to facilitate a relatively long hair growth phase.
36329527	2	103	theme	inefficient	436:446	arg1	process					472:478	the associated inefficient and tedious preparation process	421:478	the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells	421:573	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	1	104	contain	has	226:228	arg1	engineering					190:200	BACKGROUND Tissue engineering	172:200	BACKGROUND Tissue engineering of hair follicles (HFs)	172:224	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	1	104	contain	has	226:228	arg2	potential					239:247	enormous potential	230:247	enormous potential for hair loss treatment	230:271	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36329527	8	105	theme	PRP-loaded	1404:1413	arg1	IGMs					1415:1418	PRP-loaded IGMs	1404:1418	PRP-loaded IGMs	1404:1418	Importantly, PRP-loaded IGMs presented a higher hair inducibility of DPCs in vitro compared to the control and IGMs group (p < 0.05).
36329527	2	106	theme	papilla	344:350	arg1	viability					363:371	the dermal papilla cell (DPC) viability	333:371	the dermal papilla cell (DPC) viability	333:371	However, certain challenges remain, including weakening of the dermal papilla cell (DPC) viability, proliferation, and HF inducibility, as well as the associated inefficient and tedious preparation process required to generate extracellular matrix (ECM)-mimicking substrates for biomolecules or cells.
36329527	7	107	theme	good	1278:1281	arg1	viability					1283:1291	good viability	1278:1291	good viability (> 90%)	1278:1299	DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group).
36329527	7	107	theme	good	1278:1281	arg1	%					1298:1298	> 90%	1294:1298	> 90%	1294:1298	DPCs seeded on PRP-loaded IGMs exhibited good viability (> 90%), adhesion, spreading, and proliferative properties (1.2-fold greater than control group).
36329527	8	108	theme	higher	1432:1437	arg1	inducibility					1444:1455	a higher hair inducibility	1430:1455	a higher hair inducibility of DPCs	1430:1463	Importantly, PRP-loaded IGMs presented a higher hair inducibility of DPCs in vitro compared to the control and IGMs group (p < 0.05).
36329527	1	109	theme	hair	253:256	arg1	treatment					263:271	hair loss treatment	253:271	hair loss treatment	253:271	BACKGROUND Tissue engineering of hair follicles (HFs) has enormous potential for hair loss treatment.
36460010	0	0	theme	predatory	64:72	arg1	nematode					74:81	a predatory nematode	62:81	a predatory nematode	62:81	Chitin contributes to the formation of a feeding structure in a predatory nematode.
36460010	10	1	theme	chitin	1284:1289	arg1	role					1276:1279	key role	1272:1279	key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes	1272:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	5	2	theme	chitin-synthase	766:780	arg1	domain					782:787	the chitin-synthase domain	762:787	the chitin-synthase domain	762:787	Generating CRISPR-Cas9-based gene knockouts, we found that Ppa-chs-2 mutations that eliminate the chitin-synthase domain are lethal.
36460010	8	3	theme	Teethless	1019:1027	arg1	worms					1029:1033	Teethless worms	1019:1033	Teethless worms	1019:1033	Teethless worms can feed on various bacterial food sources but are incapable of predation.
36460010	7	4	theme	chitin-synthase	945:959	arg1	Z					982:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	Similarly, treatment with the chitin-synthase inhibitor Nikkomycin Z also results in teethless animals.
36460010	2	5	from	formation	294:302	arg1	pacificus					344:352	Pristionchus pacificus	331:352	Pristionchus pacificus	331:352	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	2	5	from	formation	294:302	arg1	system					363:368	a model system	355:368	a model system for studying predation and feeding structure plasticity	355:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	7	6	theme	inhibitor	961:969	arg1	Z					982:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	Similarly, treatment with the chitin-synthase inhibitor Nikkomycin Z also results in teethless animals.
36460010	1	7	theme	nematode	89:96	arg1	predators					98:106	Some nematode predators	84:106	Some nematode predators	84:106	Some nematode predators and parasites form teeth-like denticles that are histologically different from vertebrate teeth, but their biochemical composition remains elusive.
36460010	10	8	theme	apparatus	1356:1364	arg1	formation					1298:1306	the formation	1294:1306	the formation of teeth-like denticles and the complex feeding apparatus in nematodes	1294:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	6	9	theme	severe	881:886	arg1	malformation					888:899	severe malformation	881:899	severe malformation of the mouth	881:912	However, mutations in the C terminus result in viable but teethless worms, with severe malformation of the mouth.
36460010	9	10	theme	developmental	1217:1229	arg1	Ppa-eud-1					1238:1246	the sulfatase-encoding developmental switch Ppa-eud-1	1194:1246	the sulfatase-encoding developmental switch Ppa-eud-1	1194:1246	High-resolution transcriptomics revealed that Ppa-chs-2 expression is controlled by the sulfatase-encoding developmental switch Ppa-eud-1.
36460010	5	11	theme	Ppa-chs-2	727:735	arg1	mutations					737:745	Ppa-chs-2 mutations	727:745	Ppa-chs-2 mutations that eliminate the chitin-synthase domain	727:787	Generating CRISPR-Cas9-based gene knockouts, we found that Ppa-chs-2 mutations that eliminate the chitin-synthase domain are lethal.
36460010	8	12	theme	various	1047:1053	arg1	sources					1070:1076	various bacterial food sources	1047:1076	various bacterial food sources	1047:1076	Teethless worms can feed on various bacterial food sources but are incapable of predation.
36460010	9	13	theme	switch	1231:1236	arg1	Ppa-eud-1					1238:1246	the sulfatase-encoding developmental switch Ppa-eud-1	1194:1246	the sulfatase-encoding developmental switch Ppa-eud-1	1194:1246	High-resolution transcriptomics revealed that Ppa-chs-2 expression is controlled by the sulfatase-encoding developmental switch Ppa-eud-1.
36460010	10	14	theme	denticles	1322:1330	arg1	formation					1298:1306	the formation	1294:1306	the formation of teeth-like denticles and the complex feeding apparatus in nematodes	1294:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	10	15	from	nematodes	1369:1377	arg1	formation					1298:1306	the formation	1294:1306	the formation of teeth-like denticles and the complex feeding apparatus in nematodes	1294:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	10	16	from	role	1276:1279	arg1	formation					1298:1306	the formation	1294:1306	the formation of teeth-like denticles and the complex feeding apparatus in nematodes	1294:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	10	17	theme	teeth-like	1311:1320	arg1	denticles					1322:1330	teeth-like denticles	1311:1330	teeth-like denticles	1311:1330	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	0	18	from	formation	26:34	arg1	nematode					74:81	a predatory nematode	62:81	a predatory nematode	62:81	Chitin contributes to the formation of a feeding structure in a predatory nematode.
36460010	8	19	theme	food	1065:1068	arg1	sources					1070:1076	various bacterial food sources	1047:1076	various bacterial food sources	1047:1076	Teethless worms can feed on various bacterial food sources but are incapable of predation.
36460010	6	20	from	mutations	810:818	arg1	terminus					829:836	the C terminus	823:836	the C terminus	823:836	However, mutations in the C terminus result in viable but teethless worms, with severe malformation of the mouth.
36460010	8	21	theme	bacterial	1055:1063	arg1	sources					1070:1076	various bacterial food sources	1047:1076	various bacterial food sources	1047:1076	Teethless worms can feed on various bacterial food sources but are incapable of predation.
36460010	9	22	theme	High-resolution	1110:1124	arg1	transcriptomics					1126:1140	High-resolution transcriptomics	1110:1140	High-resolution transcriptomics	1110:1140	High-resolution transcriptomics revealed that Ppa-chs-2 expression is controlled by the sulfatase-encoding developmental switch Ppa-eud-1.
36460010	2	23	theme	model	357:361	arg1	pacificus					344:352	Pristionchus pacificus	331:352	Pristionchus pacificus	331:352	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	2	23	theme	model	357:361	arg1	system					363:368	a model system	355:368	a model system for studying predation and feeding structure plasticity	355:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	5	24	theme	gene	697:700	arg1	knockouts					702:710	CRISPR-Cas9-based gene knockouts	679:710	CRISPR-Cas9-based gene knockouts	679:710	Generating CRISPR-Cas9-based gene knockouts, we found that Ppa-chs-2 mutations that eliminate the chitin-synthase domain are lethal.
36460010	9	25	theme	sulfatase-encoding	1198:1215	arg1	Ppa-eud-1					1238:1246	the sulfatase-encoding developmental switch Ppa-eud-1	1194:1246	the sulfatase-encoding developmental switch Ppa-eud-1	1194:1246	High-resolution transcriptomics revealed that Ppa-chs-2 expression is controlled by the sulfatase-encoding developmental switch Ppa-eud-1.
36460010	10	26	theme	feeding	1348:1354	arg1	apparatus					1356:1364	the complex feeding apparatus	1336:1364	the complex feeding apparatus in nematodes	1336:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	2	27	theme	structure	405:413	arg1	plasticity					415:424	predation and feeding structure plasticity	383:424	plasticity	415:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	6	28	theme	teethless	859:867	arg1	worms					869:873	viable but teethless worms	848:873	viable but teethless worms	848:873	However, mutations in the C terminus result in viable but teethless worms, with severe malformation of the mouth.
36460010	2	29	theme	chitin	280:285	arg1	role					272:275	a role	270:275	a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity	270:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	10	30	theme	complex	1340:1346	arg1	apparatus					1356:1364	the complex feeding apparatus	1336:1364	the complex feeding apparatus in nematodes	1336:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	2	31	theme	feeding	397:403	arg1	plasticity					415:424	predation and feeding structure plasticity	383:424	plasticity	415:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	4	32	theme	chitin	554:559	arg1	genome					535:540	The P. pacificus genome	518:540	The P. pacificus genome	518:540	The P. pacificus genome encodes two chitin synthases, with the highly conserved chs-2 gene being composed of 60 exons forming at least four isoforms.
36460010	4	32	theme	chitin	554:559	arg1	synthases					561:569	two chitin synthases	550:569	two chitin synthases	550:569	The P. pacificus genome encodes two chitin synthases, with the highly conserved chs-2 gene being composed of 60 exons forming at least four isoforms.
36460010	7	33	with	treatment	926:934	arg1	Z					982:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	Similarly, treatment with the chitin-synthase inhibitor Nikkomycin Z also results in teethless animals.
36460010	1	34	theme	vertebrate	187:196	arg1	teeth					198:202	vertebrate teeth	187:202	vertebrate teeth	187:202	Some nematode predators and parasites form teeth-like denticles that are histologically different from vertebrate teeth, but their biochemical composition remains elusive.
36460010	9	35	theme	Ppa-chs-2	1156:1164	arg1	expression					1166:1175	Ppa-chs-2 expression	1156:1175	Ppa-chs-2 expression	1156:1175	High-resolution transcriptomics revealed that Ppa-chs-2 expression is controlled by the sulfatase-encoding developmental switch Ppa-eud-1.
36460010	6	36	theme	viable	848:853	arg1	worms					869:873	viable but teethless worms	848:873	viable but teethless worms	848:873	However, mutations in the C terminus result in viable but teethless worms, with severe malformation of the mouth.
36460010	3	37	theme	alternative	450:460	arg1	morphs					468:473	two alternative mouth morphs	446:473	two alternative mouth morphs	446:473	Pristionchus forms two alternative mouth morphs with one tooth or two teeth, respectively.
36460010	0	38	theme	feeding	41:47	arg1	structure					49:57	a feeding structure	39:57	a feeding structure	39:57	Chitin contributes to the formation of a feeding structure in a predatory nematode.
36460010	8	39	theme	predation	1099:1107	arg1	incapable					1086:1094	incapable	1086:1094	incapable	1086:1094	Teethless worms can feed on various bacterial food sources but are incapable of predation.
36460010	10	40	from	denticles	1322:1330	arg1	nematodes					1369:1377	nematodes	1369:1377	nematodes	1369:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	1	41	from	teeth	198:202	arg1	different					172:180	different	172:180	different	172:180	Some nematode predators and parasites form teeth-like denticles that are histologically different from vertebrate teeth, but their biochemical composition remains elusive.
36460010	2	42	theme	denticles	318:326	arg1	formation					294:302	the formation	290:302	the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity	290:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	4	43	theme	P. pacificus	522:533	arg1	genome					535:540	The P. pacificus genome	518:540	The P. pacificus genome	518:540	The P. pacificus genome encodes two chitin synthases, with the highly conserved chs-2 gene being composed of 60 exons forming at least four isoforms.
36460010	4	43	theme	P. pacificus	522:533	arg1	synthases					561:569	two chitin synthases	550:569	two chitin synthases	550:569	The P. pacificus genome encodes two chitin synthases, with the highly conserved chs-2 gene being composed of 60 exons forming at least four isoforms.
36460010	7	44	theme	teethless	1000:1008	arg1	animals					1010:1016	teethless animals	1000:1016	teethless animals	1000:1016	Similarly, treatment with the chitin-synthase inhibitor Nikkomycin Z also results in teethless animals.
36460010	2	45	theme	teeth-like	307:316	arg1	denticles					318:326	teeth-like denticles	307:326	teeth-like denticles	307:326	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	10	46	from	formation	1298:1306	arg1	nematodes					1369:1377	nematodes	1369:1377	nematodes	1369:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	1	47	theme	biochemical	215:225	arg1	composition					227:237	their biochemical composition	209:237	their biochemical composition	209:237	Some nematode predators and parasites form teeth-like denticles that are histologically different from vertebrate teeth, but their biochemical composition remains elusive.
36460010	5	48	theme	CRISPR-Cas9-based	679:695	arg1	knockouts					702:710	CRISPR-Cas9-based gene knockouts	679:710	CRISPR-Cas9-based gene knockouts	679:710	Generating CRISPR-Cas9-based gene knockouts, we found that Ppa-chs-2 mutations that eliminate the chitin-synthase domain are lethal.
36460010	6	49	theme	C	827:827	arg1	terminus					829:836	the C terminus	823:836	the C terminus	823:836	However, mutations in the C terminus result in viable but teethless worms, with severe malformation of the mouth.
36460010	10	50	theme	key	1272:1274	arg1	role					1276:1279	key role	1272:1279	key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes	1272:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	4	51	theme	conserved	588:596	arg1	gene					604:607	the highly conserved chs-2 gene	577:607	the highly conserved chs-2 gene	577:607	The P. pacificus genome encodes two chitin synthases, with the highly conserved chs-2 gene being composed of 60 exons forming at least four isoforms.
36460010	2	52	theme	predation	383:391	arg1	plasticity					415:424	predation and feeding structure plasticity	383:424	plasticity	415:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	10	53	from	apparatus	1356:1364	arg1	nematodes					1369:1377	nematodes	1369:1377	nematodes	1369:1377	This study indicates a key role of chitin in the formation of teeth-like denticles and the complex feeding apparatus in nematodes.
36460010	3	54	theme	mouth	462:466	arg1	morphs					468:473	two alternative mouth morphs	446:473	two alternative mouth morphs	446:473	Pristionchus forms two alternative mouth morphs with one tooth or two teeth, respectively.
36460010	1	55	theme	teeth-like	127:136	arg1	denticles					138:146	teeth-like denticles	127:146	teeth-like denticles that are histologically different from vertebrate teeth	127:202	Some nematode predators and parasites form teeth-like denticles that are histologically different from vertebrate teeth, but their biochemical composition remains elusive.
36460010	2	56	from	role	272:275	arg1	formation					294:302	the formation	290:302	the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity	290:424	Here, we show a role of chitin in the formation of teeth-like denticles in Pristionchus pacificus, a model system for studying predation and feeding structure plasticity.
36460010	0	57	theme	structure	49:57	arg1	formation					26:34	the formation	22:34	the formation of a feeding structure in a predatory nematode	22:81	Chitin contributes to the formation of a feeding structure in a predatory nematode.
36460010	6	58	theme	mouth	908:912	arg1	malformation					888:899	severe malformation	881:899	severe malformation of the mouth	881:912	However, mutations in the C terminus result in viable but teethless worms, with severe malformation of the mouth.
36460010	7	59	theme	Nikkomycin	971:980	arg1	Z					982:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	the chitin-synthase inhibitor Nikkomycin Z	941:982	Similarly, treatment with the chitin-synthase inhibitor Nikkomycin Z also results in teethless animals.
36460010	4	60	theme	chs-2	598:602	arg1	gene					604:607	the highly conserved chs-2 gene	577:607	the highly conserved chs-2 gene	577:607	The P. pacificus genome encodes two chitin synthases, with the highly conserved chs-2 gene being composed of 60 exons forming at least four isoforms.
35917175	9	0	theme	acids	1270:1274	arg1	biosynthesis					1223:1234	the biosynthesis	1219:1234	the biosynthesis of amino acids, short-chain fatty acids, and L-arginine	1219:1290	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	2	1	theme	particular	334:343	arg1	effect					345:350	a particular effect	332:350	a particular effect on Treg homeostasis	332:370	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	9	2	theme	L-arginine	1281:1290	arg1	biosynthesis					1223:1234	the biosynthesis	1219:1234	the biosynthesis of amino acids, short-chain fatty acids, and L-arginine	1219:1290	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	2	3	contain	has	263:265	arg2	effect					276:281	a direct effect	267:281	a direct effect	267:281	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	2	3	contain	has	263:265	arg1	composition					251:261	Gut microbiota composition	236:261	Gut microbiota composition	236:261	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	5	4	used	used	587:590	arg2	We					584:585	We	584:585	We	584:585	We used 16S ribosomal RNA profiling and metagenomics to characterize gut microbiota of mice and humans treated or not with IL-2LD.
35917175	6	5	theme	diabetes	871:878	arg1	models					840:845	models	840:845	models of gut inflammation and diabetes	840:878	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	8	6	theme	sodium-induced	1042:1055	arg1	colitis					1057:1063	dextran sulfate sodium-induced colitis	1026:1063	dextran sulfate sodium-induced colitis	1026:1063	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	10	7	theme	IL-2LD	1416:1421	arg1	effects					1405:1411	the immunoregulatory effects	1384:1411	the immunoregulatory effects of IL-2LD	1384:1421	Our results demonstrate that IL-2LD induced changes in gut microbiota that are involved in the immunoregulatory effects of IL-2LD and suggest a crosstalk between Tregs and gut microbiota.
35917175	2	8	contain	has	328:330	arg1	it					325:326	it	325:326	it	325:326	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	2	8	contain	has	328:330	arg2	effect					345:350	a particular effect	332:350	a particular effect on Treg homeostasis	332:370	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	6	9	theme	IL-2LD-treated	772:785	arg1	mice					806:809	IL-2LD-treated to naive recipient mice	772:809	IL-2LD-treated to naive recipient mice	772:809	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	8	10	theme	sulfate	1034:1040	arg1	colitis					1057:1063	dextran sulfate sodium-induced colitis	1026:1063	dextran sulfate sodium-induced colitis	1026:1063	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	6	11	theme	inflammation	854:865	arg1	models					840:845	models	840:845	models of gut inflammation and diabetes	840:878	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	8	12	theme	C57BL/6J	1007:1014	arg1	mice					1016:1019	C57BL/6J mice	1007:1019	C57BL/6J mice	1007:1019	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	2	13	theme	immune	290:295	arg1	system					297:302	the immune system	286:302	the immune system	286:302	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	11	14	theme	action	1559:1564	arg1	mode					1551:1554	the mode	1547:1554	the mode of action of Treg-directed therapies	1547:1591	These results provide potentially novel insight for understanding the mode of action of Treg-directed therapies.
35917175	6	15	theme	gut	850:852	arg1	inflammation					854:865	gut inflammation	850:865	gut inflammation	850:865	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	8	16	theme	dextran	1026:1032	arg1	sulfate					1034:1040	dextran sulfate	1026:1040	dextran sulfate sodium-induced colitis	1026:1063	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	11	17	theme	novel	1515:1519	arg1	insight					1521:1527	potentially novel insight	1503:1527	potentially novel insight for understanding the mode of action of Treg-directed therapies	1503:1591	These results provide potentially novel insight for understanding the mode of action of Treg-directed therapies.
35917175	5	18	theme	16S	592:594	arg1	RNA					606:608	16S ribosomal RNA	592:608	16S ribosomal RNA profiling	592:618	We used 16S ribosomal RNA profiling and metagenomics to characterize gut microbiota of mice and humans treated or not with IL-2LD.
35917175	7	19	theme	gut	906:908	arg1	composition					921:931	gut microbiota composition	906:931	gut microbiota composition	906:931	IL-2LD markedly affected gut microbiota composition in mice and humans.
35917175	0	20	theme	Low-dose	0:7	arg1	IL-2					9:12	Low-dose IL-2	0:12	Low-dose IL-2	0:12	Low-dose IL-2 shapes a tolerogenic gut microbiota that improves autoimmunity and gut inflammation.
35917175	6	21	dep	mice	806:809	arg1	to					787:788	to	787:788	to	787:788	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	5	22	theme	ribosomal	596:604	arg1	RNA					606:608	16S ribosomal RNA	592:608	16S ribosomal RNA profiling	592:618	We used 16S ribosomal RNA profiling and metagenomics to characterize gut microbiota of mice and humans treated or not with IL-2LD.
35917175	7	23	theme	microbiota	910:919	arg1	composition					921:931	gut microbiota composition	906:931	gut microbiota composition	906:931	IL-2LD markedly affected gut microbiota composition in mice and humans.
35917175	3	24	theme	Low-dose	373:380	arg1	IL-2LD					388:393	IL-2LD	388:393	IL-2LD	388:393	Low-dose IL-2 (IL-2LD) stimulates Tregs and is a promising treatment for autoimmune and inflammatory diseases.
35917175	3	24	theme	Low-dose	373:380	arg1	IL-2					382:385	Low-dose IL-2	373:385	Low-dose IL-2 (IL-2LD)	373:394	Low-dose IL-2 (IL-2LD) stimulates Tregs and is a promising treatment for autoimmune and inflammatory diseases.
35917175	5	25	theme	RNA	606:608	arg1	profiling					610:618	16S ribosomal RNA profiling	592:618	16S ribosomal RNA profiling	592:618	We used 16S ribosomal RNA profiling and metagenomics to characterize gut microbiota of mice and humans treated or not with IL-2LD.
35917175	10	26	theme	immunoregulatory	1388:1403	arg1	effects					1405:1411	the immunoregulatory effects	1384:1411	the immunoregulatory effects of IL-2LD	1384:1421	Our results demonstrate that IL-2LD induced changes in gut microbiota that are involved in the immunoregulatory effects of IL-2LD and suggest a crosstalk between Tregs and gut microbiota.
35917175	2	27	theme	Treg	355:358	arg1	homeostasis					360:370	Treg homeostasis	355:370	Treg homeostasis	355:370	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	4	28	theme	IL-2LD	519:524	arg1	impact					509:514	the impact	505:514	the impact of IL-2LD on gut microbiota and correlatively on the immune system	505:581	We aimed to evaluate the impact of IL-2LD on gut microbiota and correlatively on the immune system.
35917175	5	29	theme	mice	671:674	arg1	microbiota					657:666	gut microbiota	653:666	gut microbiota of mice and humans treated or not with IL-2LD	653:712	We used 16S ribosomal RNA profiling and metagenomics to characterize gut microbiota of mice and humans treated or not with IL-2LD.
35917175	2	30	from	effect	345:350	arg1	homeostasis					360:370	Treg homeostasis	355:370	Treg homeostasis	355:370	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	0	31	dep	autoimmunity	64:75	arg1	inflammation					85:96	inflammation	85:96	inflammation	85:96	Low-dose IL-2 shapes a tolerogenic gut microbiota that improves autoimmunity and gut inflammation.
35917175	8	32	theme	microbiota	979:988	arg1	Transfer					953:960	Transfer	953:960	Transfer of an IL-2-tuned microbiota by FMT	953:995	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	1	33	theme	microbiota	103:112	arg1	dysbiosis					114:122	Gut microbiota dysbiosis	99:122	Gut microbiota dysbiosis	99:122	Gut microbiota dysbiosis is associated with inflammatory bowel diseases and with cardiometabolic, neurological, and autoimmune diseases.
35917175	10	34	from	changes	1337:1343	arg1	microbiota					1352:1361	gut microbiota	1348:1361	gut microbiota	1348:1361	Our results demonstrate that IL-2LD induced changes in gut microbiota that are involved in the immunoregulatory effects of IL-2LD and suggest a crosstalk between Tregs and gut microbiota.
35917175	0	35	theme	gut	35:37	arg1	microbiota					39:48	a tolerogenic gut microbiota	21:48	a tolerogenic gut microbiota that improves autoimmunity and gut inflammation	21:96	Low-dose IL-2 shapes a tolerogenic gut microbiota that improves autoimmunity and gut inflammation.
35917175	4	36	theme	gut	529:531	arg1	microbiota					533:542	gut microbiota	529:542	gut microbiota	529:542	We aimed to evaluate the impact of IL-2LD on gut microbiota and correlatively on the immune system.
35917175	5	37	theme	humans	680:685	arg1	microbiota					657:666	gut microbiota	653:666	gut microbiota of mice and humans treated or not with IL-2LD	653:712	We used 16S ribosomal RNA profiling and metagenomics to characterize gut microbiota of mice and humans treated or not with IL-2LD.
35917175	1	38	theme	autoimmune	215:224	arg1	diseases					226:233	autoimmune diseases	215:233	autoimmune diseases	215:233	Gut microbiota dysbiosis is associated with inflammatory bowel diseases and with cardiometabolic, neurological, and autoimmune diseases.
35917175	0	39	theme	tolerogenic	23:33	arg1	microbiota					39:48	a tolerogenic gut microbiota	21:48	a tolerogenic gut microbiota that improves autoimmunity and gut inflammation	21:96	Low-dose IL-2 shapes a tolerogenic gut microbiota that improves autoimmunity and gut inflammation.
35917175	4	40	from	impact	509:514	arg1	system					576:581	the immune system	565:581	the immune system	565:581	We aimed to evaluate the impact of IL-2LD on gut microbiota and correlatively on the immune system.
35917175	4	40	from	impact	509:514	arg1	microbiota					533:542	gut microbiota	529:542	gut microbiota	529:542	We aimed to evaluate the impact of IL-2LD on gut microbiota and correlatively on the immune system.
35917175	9	41	theme	Metagenomic	1101:1111	arg1	analyses					1113:1120	Metagenomic analyses	1101:1120	Metagenomic analyses	1101:1120	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	2	42	theme	microbiota	240:249	arg1	composition					251:261	Gut microbiota composition	236:261	Gut microbiota composition	236:261	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	6	43	theme	fecal	728:732	arg1	FMT					762:764	FMT	762:764	FMT	762:764	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	6	43	theme	fecal	728:732	arg1	transplantation					745:759	fecal microbiota transplantation	728:759	fecal microbiota transplantation (FMT)	728:765	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	3	44	theme	promising	422:430	arg1	treatment					432:440	a promising treatment	420:440	a promising treatment for autoimmune and inflammatory diseases	420:481	Low-dose IL-2 (IL-2LD) stimulates Tregs and is a promising treatment for autoimmune and inflammatory diseases.
35917175	9	45	theme	short-chain	1252:1262	arg1	acids					1270:1274	short-chain fatty acids	1252:1274	short-chain fatty acids	1252:1274	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	8	46	theme	NOD	1091:1093	arg1	mice					1095:1098	NOD mice	1091:1098	NOD mice	1091:1098	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	2	47	theme	Gut	236:238	arg1	composition					251:261	Gut microbiota composition	236:261	Gut microbiota composition	236:261	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	3	48	theme	inflammatory	461:472	arg1	diseases					474:481	autoimmune and inflammatory diseases	446:481	autoimmune and inflammatory diseases	446:481	Low-dose IL-2 (IL-2LD) stimulates Tregs and is a promising treatment for autoimmune and inflammatory diseases.
35917175	10	49	theme	gut	1465:1467	arg1	microbiota					1469:1478	gut microbiota	1465:1478	gut microbiota	1465:1478	Our results demonstrate that IL-2LD induced changes in gut microbiota that are involved in the immunoregulatory effects of IL-2LD and suggest a crosstalk between Tregs and gut microbiota.
35917175	5	50	theme	gut	653:655	arg1	microbiota					657:666	gut microbiota	653:666	gut microbiota of mice and humans treated or not with IL-2LD	653:712	We used 16S ribosomal RNA profiling and metagenomics to characterize gut microbiota of mice and humans treated or not with IL-2LD.
35917175	9	51	theme	amino	1239:1243	arg1	acids					1245:1249	amino acids	1239:1249	amino acids	1239:1249	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	9	52	theme	acids	1245:1249	arg1	biosynthesis					1223:1234	the biosynthesis	1219:1234	the biosynthesis of amino acids, short-chain fatty acids, and L-arginine	1219:1290	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	11	53	theme	Treg-directed	1569:1581	arg1	therapies					1583:1591	Treg-directed therapies	1569:1591	Treg-directed therapies	1569:1591	These results provide potentially novel insight for understanding the mode of action of Treg-directed therapies.
35917175	6	54	theme	microbiota	734:743	arg1	FMT					762:764	FMT	762:764	FMT	762:764	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	6	54	theme	microbiota	734:743	arg1	transplantation					745:759	fecal microbiota transplantation	728:759	fecal microbiota transplantation (FMT)	728:765	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	8	55	theme	IL-2-tuned	968:977	arg1	microbiota					979:988	an IL-2-tuned microbiota	965:988	an IL-2-tuned microbiota	965:988	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	11	56	theme	therapies	1583:1591	arg1	action					1559:1564	action	1559:1564	action of Treg-directed therapies	1559:1591	These results provide potentially novel insight for understanding the mode of action of Treg-directed therapies.
35917175	3	57	theme	autoimmune	446:455	arg1	diseases					474:481	autoimmune and inflammatory diseases	446:481	autoimmune and inflammatory diseases	446:481	Low-dose IL-2 (IL-2LD) stimulates Tregs and is a promising treatment for autoimmune and inflammatory diseases.
35917175	1	58	theme	Gut	99:101	arg1	dysbiosis					114:122	Gut microbiota dysbiosis	99:122	Gut microbiota dysbiosis	99:122	Gut microbiota dysbiosis is associated with inflammatory bowel diseases and with cardiometabolic, neurological, and autoimmune diseases.
35917175	8	59	from	diabetes	1079:1086	arg1	mice					1095:1098	NOD mice	1091:1098	NOD mice	1091:1098	Transfer of an IL-2-tuned microbiota by FMT protected C57BL/6J mice from dextran sulfate sodium-induced colitis and prevented diabetes in NOD mice.
35917175	6	60	theme	recipient	796:804	arg1	mice					806:809	IL-2LD-treated to naive recipient mice	772:809	IL-2LD-treated to naive recipient mice	772:809	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	2	61	theme	direct	269:274	arg1	effect					276:281	a direct effect	267:281	a direct effect	267:281	Gut microbiota composition has a direct effect on the immune system, and vice versa, and it has a particular effect on Treg homeostasis.
35917175	4	62	theme	immune	569:574	arg1	system					576:581	the immune system	565:581	the immune system	565:581	We aimed to evaluate the impact of IL-2LD on gut microbiota and correlatively on the immune system.
35917175	6	63	theme	naive	790:794	arg1	mice					806:809	IL-2LD-treated to naive recipient mice	772:809	IL-2LD-treated to naive recipient mice	772:809	We performed fecal microbiota transplantation (FMT) from IL-2LD-treated to naive recipient mice and evaluated its effects in models of gut inflammation and diabetes.
35917175	10	64	theme	gut	1348:1350	arg1	microbiota					1352:1361	gut microbiota	1348:1361	gut microbiota	1348:1361	Our results demonstrate that IL-2LD induced changes in gut microbiota that are involved in the immunoregulatory effects of IL-2LD and suggest a crosstalk between Tregs and gut microbiota.
35917175	1	65	theme	inflammatory	143:154	arg1	diseases					162:169	inflammatory bowel diseases	143:169	inflammatory bowel diseases	143:169	Gut microbiota dysbiosis is associated with inflammatory bowel diseases and with cardiometabolic, neurological, and autoimmune diseases.
35917175	9	66	theme	microbial	1188:1196	arg1	pathways					1198:1205	microbial pathways	1188:1205	microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine	1188:1290	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	9	67	theme	fatty	1264:1268	arg1	acids					1270:1274	short-chain fatty acids	1252:1274	short-chain fatty acids	1252:1274	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	9	68	theme	several	1145:1151	arg1	species					1153:1159	several species	1145:1159	several species affected by IL-2LD	1145:1178	Metagenomic analyses highlighted a role for several species affected by IL-2LD and for microbial pathways involved in the biosynthesis of amino acids, short-chain fatty acids, and L-arginine.
35917175	1	69	theme	bowel	156:160	arg1	diseases					162:169	inflammatory bowel diseases	143:169	inflammatory bowel diseases	143:169	Gut microbiota dysbiosis is associated with inflammatory bowel diseases and with cardiometabolic, neurological, and autoimmune diseases.
35305322	5	0	dep	nanosheets	807:816	arg1	%					829:829	2%	828:829	2%	828:829	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	5	0	dep	nanosheets	807:816	arg1	%					822:822	0.5%	819:822	0.5%	819:822	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	6	1	theme	myogenic	1195:1202	arg1	differentiation					1204:1218	myogenic differentiation	1195:1218	myogenic differentiation	1195:1218	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	8	2	theme	nanohybrid	1652:1661	arg1	hydrogels					1663:1671	the nanohybrid hydrogels	1648:1671	the nanohybrid hydrogels	1648:1671	The incorporation of rGO significantly increases the electrical conductivity of the nanohybrid hydrogels, with values in the range of muscle tissue.
35305322	10	3	theme	great	1983:1987	arg1	potential					1989:1997	great potential	1983:1997	great potential for biomedical applications related to the regeneration of electroactive tissues	1983:2078	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	2	4	theme	muscle	323:328	arg1	cells					330:334	muscle cells	323:334	muscle cells	323:334	Electrical conductivity is a crucial factor for muscle cells, which are electrically active.
35305322	8	5	theme	tissue	1709:1714	arg1	range					1693:1697	the range	1689:1697	the range of muscle tissue	1689:1714	The incorporation of rGO significantly increases the electrical conductivity of the nanohybrid hydrogels, with values in the range of muscle tissue.
35305322	7	6	theme	SA	1428:1429	arg1	chains					1431:1436	SA chains	1428:1436	SA chains	1428:1436	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	6	7	theme	surface	1066:1072	arg1	wettability					1074:1084	surface wettability	1066:1084	surface wettability	1066:1084	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	4	8	theme	stability	668:676	arg1	adhesion					633:640	the low cell adhesion	620:640	the low cell adhesion of SA and weak structural stability	620:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	1	9	theme	novel	216:220	arg1	biomaterials					222:233	novel biomaterials	216:233	novel biomaterials for their unique electrical properties	216:272	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	6	10	theme	electrical	1152:1161	arg1	conductivity					1163:1174	electrical conductivity	1152:1174	electrical conductivity	1152:1174	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	4	11	theme	SA	645:646	arg1	adhesion					633:640	the low cell adhesion	620:640	the low cell adhesion of SA and weak structural stability	620:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	6	12	theme	thermal	1087:1093	arg1	behavior					1095:1102	thermal behavior	1087:1102	thermal behavior	1087:1102	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	4	13	theme	weak	652:655	arg1	stability					668:676	weak structural stability	652:676	weak structural stability	652:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	1	14	theme	unique	245:250	arg1	properties					263:272	their unique electrical properties	239:272	their unique electrical properties	239:272	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	4	15	theme	PCL	720:722	arg1	incorporation					703:715	the incorporation	699:715	the incorporation of PCL	699:722	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	1	16	theme	graphene	169:176	arg1	oxide					178:182	reduced graphene oxide	161:182	reduced graphene oxide (rGO)	161:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	16	theme	graphene	169:176	arg1	rGO					185:187	rGO	185:187	rGO	185:187	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	17	used	used	194:197	arg2	derivatives					141:151	Graphene derivatives	132:151	Graphene derivatives such as reduced graphene oxide (rGO)	132:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	17	used	used	194:197	arg2	components					202:211	components	202:211	components of novel biomaterials for their unique electrical properties	202:272	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	17	used	used	194:197	arg2	oxide					178:182	reduced graphene oxide	161:182	reduced graphene oxide (rGO)	161:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	6	18	theme	advanced	1020:1027	arg1	materials					1029:1037	these advanced materials	1014:1037	these advanced materials	1014:1037	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	9	19	theme	murine	1746:1751	arg1	myoblasts					1753:1761	C2C12 murine myoblasts	1740:1761	C2C12 murine myoblasts	1740:1761	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	7	20	theme	Ca2+	1368:1371	arg1	ions					1373:1376	Ca2+ ions	1368:1376	Ca2+ ions (egg-box model)	1368:1392	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	7	20	theme	Ca2+	1368:1371	arg1	model					1387:1391	egg-box model	1379:1391	egg-box model	1379:1391	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	10	21	theme	electroactive	2058:2070	arg1	tissues					2072:2078	electroactive tissues	2058:2078	electroactive tissues	2058:2078	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	0	22	theme	muscle	105:110	arg1	engineering					119:129	skeletal muscle tissue engineering	96:129	skeletal muscle tissue engineering	96:129	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid hydrogels for skeletal muscle tissue engineering.
35305322	5	23	theme	electroactive	864:876	arg1	biomaterials					899:910	electroactive nanohybrid composite biomaterials	864:910	electroactive nanohybrid composite biomaterials	864:910	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	5	24	theme	composite	889:897	arg1	biomaterials					899:910	electroactive nanohybrid composite biomaterials	864:910	electroactive nanohybrid composite biomaterials	864:910	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	6	25	theme	biological	989:998	arg1	properties					1000:1009	physical and biological properties	976:1009	physical and biological properties	976:1009	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	properties					1140:1149	mechanical properties	1129:1149	mechanical properties	1129:1149	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	adhesion					1182:1189	cell adhesion	1177:1189	cell adhesion	1177:1189	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	sorption					1056:1063	water sorption	1050:1063	water sorption	1050:1063	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	behavior					1095:1102	thermal behavior	1087:1102	thermal behavior	1087:1102	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	conductivity					1163:1174	electrical conductivity	1152:1174	electrical conductivity	1152:1174	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	differentiation					1204:1218	myogenic differentiation	1195:1218	myogenic differentiation	1195:1218	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	wettability					1074:1084	surface wettability	1066:1084	surface wettability	1066:1084	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	25	theme	biological	989:998	arg1	degradation					1116:1126	thermal degradation	1108:1126	thermal degradation	1108:1126	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	8	26	from	values	1679:1684	arg1	range					1693:1697	the range	1689:1697	the range of muscle tissue	1689:1714	The incorporation of rGO significantly increases the electrical conductivity of the nanohybrid hydrogels, with values in the range of muscle tissue.
35305322	6	27	theme	physical	976:983	arg1	properties					1000:1009	physical and biological properties	976:1009	physical and biological properties	976:1009	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	properties					1140:1149	mechanical properties	1129:1149	mechanical properties	1129:1149	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	adhesion					1182:1189	cell adhesion	1177:1189	cell adhesion	1177:1189	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	sorption					1056:1063	water sorption	1050:1063	water sorption	1050:1063	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	behavior					1095:1102	thermal behavior	1087:1102	thermal behavior	1087:1102	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	conductivity					1163:1174	electrical conductivity	1152:1174	electrical conductivity	1152:1174	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	differentiation					1204:1218	myogenic differentiation	1195:1218	myogenic differentiation	1195:1218	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	wettability					1074:1084	surface wettability	1066:1084	surface wettability	1066:1084	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	27	theme	physical	976:983	arg1	degradation					1116:1126	thermal degradation	1108:1126	thermal degradation	1108:1126	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	9	28	theme	nanohybrid	1792:1801	arg1	cytotoxic					1821:1829	cytotoxic	1821:1829	cytotoxic	1821:1829	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	9	28	theme	nanohybrid	1792:1801	arg1	hydrogels					1803:1811	the conductive nanohybrid hydrogels	1777:1811	the conductive nanohybrid hydrogels	1777:1811	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	3	29	theme	biodegradable	470:482	arg1	polycaprolactone					536:551	polycaprolactone	536:551	polycaprolactone (PCL)	536:557	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	29	theme	biodegradable	470:482	arg1	biomaterials					497:508	two biodegradable FDA-approved biomaterials	466:508	two biodegradable FDA-approved biomaterials	466:508	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	29	theme	biodegradable	470:482	arg1	alginate					518:525	sodium alginate	511:525	sodium alginate (SA)	511:530	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	30	theme	type	412:415	arg1	development					391:401	the development	387:401	the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker	387:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	0	31	theme	Electroactive	0:12	arg1	nanohybrid					71:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid hydrogels for skeletal muscle tissue engineering.
35305322	3	32	theme	sodium	511:516	arg1	SA					528:529	SA	528:529	SA	528:529	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	32	theme	sodium	511:516	arg1	biomaterials					497:508	two biodegradable FDA-approved biomaterials	466:508	two biodegradable FDA-approved biomaterials	466:508	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	32	theme	sodium	511:516	arg1	alginate					518:525	sodium alginate	511:525	sodium alginate (SA)	511:530	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	0	33	theme	graphene	56:63	arg1	nanohybrid					71:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid hydrogels for skeletal muscle tissue engineering.
35305322	7	34	theme	rGO	1538:1540	arg1	nanosheets					1542:1551	rGO nanosheets	1538:1551	rGO nanosheets	1538:1551	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	6	35	theme	water	1050:1054	arg1	sorption					1056:1063	water sorption	1050:1063	water sorption	1050:1063	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	3	36	theme	network	449:455	arg1	type					412:415	a new type	406:415	a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker	406:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	37	theme	semi-interpenetrated	420:439	arg1	network					449:455	semi-interpenetrated polymer network	420:455	semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker	420:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	9	38	dep	In	1717:1718	arg1	vitro					1720:1724	vitro	1720:1724	vitro	1720:1724	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	5	39	theme	rGO	803:805	arg1	nanosheets					807:816	rGO nanosheets	803:816	rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets)	803:851	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	7	40	theme	strong	1506:1511	arg1	bonding					1522:1528	strong hydrogen bonding	1506:1528	strong hydrogen bonding between rGO nanosheets and SA chains	1506:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	6	41	theme	mechanical	1129:1138	arg1	properties					1140:1149	mechanical properties	1129:1149	mechanical properties	1129:1149	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	8	42	theme	hydrogels	1663:1671	arg1	conductivity					1632:1643	the electrical conductivity	1617:1643	the electrical conductivity of the nanohybrid hydrogels	1617:1671	The incorporation of rGO significantly increases the electrical conductivity of the nanohybrid hydrogels, with values in the range of muscle tissue.
35305322	1	43	theme	electrical	252:261	arg1	properties					263:272	their unique electrical properties	239:272	their unique electrical properties	239:272	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	9	44	theme	myogenic	1877:1884	arg1	differentiation					1886:1900	myogenic differentiation	1877:1900	myogenic differentiation	1877:1900	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	7	45	theme	Ca2+	1491:1494	arg1	ions					1496:1499	Ca2+ ions	1491:1499	Ca2+ ions	1491:1499	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	4	46	theme	cell	628:631	arg1	adhesion					633:640	the low cell adhesion	620:640	the low cell adhesion of SA and weak structural stability	620:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	10	47	theme	novel	1937:1941	arg1	hydrogels					1968:1976	these novel electroactive nanohybrid hydrogels	1931:1976	these novel electroactive nanohybrid hydrogels	1931:1976	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	5	48	dep	%	822:822	arg1	nanosheets					841:850	wt/wt rGO nanosheets	831:850	wt/wt rGO nanosheets	831:850	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	7	49	theme	complex	1260:1266	arg1	nano-network					1268:1279	a complex nano-network	1258:1279	a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains	1258:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	4	50	theme	Several	594:600	arg1	drawbacks					602:610	Several drawbacks	594:610	Several drawbacks such as the low cell adhesion of SA and weak structural stability	594:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	4	50	theme	Several	594:600	arg1	adhesion					633:640	the low cell adhesion	620:640	the low cell adhesion of SA and weak structural stability	620:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	7	51	with	nano-network	1268:1279	arg1	interactions					1296:1307	different interactions	1286:1307	different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains	1286:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	3	52	theme	Ca2+	565:568	arg1	ions					570:573	Ca2+ ions	565:573	Ca2+ ions as SA crosslinker	565:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	7	53	theme	rGO	1457:1459	arg1	nanosheets					1461:1470	rGO nanosheets	1457:1470	rGO nanosheets themselves through Ca2+ ions	1457:1499	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	8	54	theme	muscle	1702:1707	arg1	tissue					1709:1714	muscle tissue	1702:1714	muscle tissue	1702:1714	The incorporation of rGO significantly increases the electrical conductivity of the nanohybrid hydrogels, with values in the range of muscle tissue.
35305322	5	55	theme	rGO	837:839	arg1	nanosheets					841:850	wt/wt rGO nanosheets	831:850	wt/wt rGO nanosheets	831:850	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	3	56	theme	polymer	441:447	arg1	network					449:455	semi-interpenetrated polymer network	420:455	semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker	420:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	6	57	theme	cell	1177:1180	arg1	adhesion					1182:1189	cell adhesion	1177:1189	cell adhesion	1177:1189	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	4	58	theme	structural	657:666	arg1	stability					668:676	weak structural stability	652:676	weak structural stability	652:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	10	59	theme	nanohybrid	1957:1966	arg1	hydrogels					1968:1976	these novel electroactive nanohybrid hydrogels	1931:1976	these novel electroactive nanohybrid hydrogels	1931:1976	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	10	60	theme	biomedical	2003:2012	arg1	applications					2014:2025	biomedical applications	2003:2025	biomedical applications related to the regeneration of electroactive tissues	2003:2078	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	3	61	with	biomaterials	497:508	arg1	ions					570:573	Ca2+ ions	565:573	Ca2+ ions as SA crosslinker	565:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	1	62	theme	biomaterials	222:233	arg1	oxide					178:182	reduced graphene oxide	161:182	reduced graphene oxide (rGO)	161:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	62	theme	biomaterials	222:233	arg1	derivatives					141:151	Graphene derivatives	132:151	Graphene derivatives such as reduced graphene oxide (rGO)	132:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	62	theme	biomaterials	222:233	arg1	components					202:211	components	202:211	components of novel biomaterials for their unique electrical properties	202:272	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	7	63	theme	rGO	1409:1411	arg1	nanosheets					1413:1422	rGO nanosheets	1409:1422	rGO nanosheets	1409:1422	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	10	64	contain	have	1978:1981	arg1	hydrogels					1968:1976	these novel electroactive nanohybrid hydrogels	1931:1976	these novel electroactive nanohybrid hydrogels	1931:1976	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	10	64	contain	have	1978:1981	arg2	potential					1989:1997	great potential	1983:1997	great potential for biomedical applications related to the regeneration of electroactive tissues	1983:2078	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	2	65	theme	Electrical	275:284	arg1	factor					312:317	a crucial factor	302:317	a crucial factor for muscle cells, which are electrically active	302:365	Electrical conductivity is a crucial factor for muscle cells, which are electrically active.
35305322	2	65	theme	Electrical	275:284	arg1	conductivity					286:297	Electrical conductivity	275:297	Electrical conductivity	275:297	Electrical conductivity is a crucial factor for muscle cells, which are electrically active.
35305322	9	66	theme	In	1717:1718	arg1	cultures					1726:1733	In vitro cultures	1717:1733	In vitro cultures with C2C12 murine myoblasts	1717:1761	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	10	67	dep	muscle	2106:2111	arg1	engineering					2120:2130	tissue engineering	2113:2130	skeletal muscle tissue engineering	2097:2130	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	1	68	theme	reduced	161:167	arg1	oxide					178:182	reduced graphene oxide	161:182	reduced graphene oxide (rGO)	161:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	68	theme	reduced	161:167	arg1	rGO					185:187	rGO	185:187	rGO	185:187	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	10	69	theme	related	2027:2033	arg1	applications					2014:2025	biomedical applications	2003:2025	biomedical applications related to the regeneration of electroactive tissues	2003:2078	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	2	70	theme	crucial	304:310	arg1	factor					312:317	a crucial factor	302:317	a crucial factor for muscle cells, which are electrically active	302:365	Electrical conductivity is a crucial factor for muscle cells, which are electrically active.
35305322	2	70	theme	crucial	304:310	arg1	conductivity					286:297	Electrical conductivity	275:297	Electrical conductivity	275:297	Electrical conductivity is a crucial factor for muscle cells, which are electrically active.
35305322	6	71	theme	materials	1029:1037	arg1	properties					1000:1009	physical and biological properties	976:1009	physical and biological properties	976:1009	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	properties					1140:1149	mechanical properties	1129:1149	mechanical properties	1129:1149	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	adhesion					1182:1189	cell adhesion	1177:1189	cell adhesion	1177:1189	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	sorption					1056:1063	water sorption	1050:1063	water sorption	1050:1063	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	behavior					1095:1102	thermal behavior	1087:1102	thermal behavior	1087:1102	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	conductivity					1163:1174	electrical conductivity	1152:1174	electrical conductivity	1152:1174	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	differentiation					1204:1218	myogenic differentiation	1195:1218	myogenic differentiation	1195:1218	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	wettability					1074:1084	surface wettability	1066:1084	surface wettability	1066:1084	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	71	theme	materials	1029:1037	arg1	degradation					1116:1126	thermal degradation	1108:1126	thermal degradation	1108:1126	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	9	72	theme	C2C12	1740:1744	arg1	myoblasts					1753:1761	C2C12 murine myoblasts	1740:1761	C2C12 murine myoblasts	1740:1761	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	7	73	theme	egg-box	1379:1385	arg1	ions					1373:1376	Ca2+ ions	1368:1376	Ca2+ ions (egg-box model)	1368:1392	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	7	73	theme	egg-box	1379:1385	arg1	model					1387:1391	egg-box model	1379:1391	egg-box model	1379:1391	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	10	74	theme	tissues	2072:2078	arg1	regeneration					2042:2053	the regeneration	2038:2053	the regeneration of electroactive tissues	2038:2078	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	0	75	theme	tissue	112:117	arg1	engineering					119:129	skeletal muscle tissue engineering	96:129	skeletal muscle tissue engineering	96:129	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid hydrogels for skeletal muscle tissue engineering.
35305322	5	76	theme	nanohybrid	878:887	arg1	biomaterials					899:910	electroactive nanohybrid composite biomaterials	864:910	electroactive nanohybrid composite biomaterials	864:910	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	6	77	theme	properties	1000:1009	arg1	microstructure					938:951	the microstructure	934:951	the microstructure	934:951	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	6	77	theme	properties	1000:1009	arg1	enhancement					961:971	the enhancement	957:971	the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation	957:1218	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	10	78	theme	skeletal	2097:2104	arg1	muscle					2106:2111	skeletal muscle tissue engineering	2097:2130	skeletal muscle tissue engineering	2097:2130	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	3	79	theme	SA	578:579	arg1	crosslinker					581:591	SA crosslinker	578:591	SA crosslinker	578:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	9	80	theme	conductive	1781:1790	arg1	cytotoxic					1821:1829	cytotoxic	1821:1829	cytotoxic	1821:1829	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	9	80	theme	conductive	1781:1790	arg1	hydrogels					1803:1811	the conductive nanohybrid hydrogels	1777:1811	the conductive nanohybrid hydrogels	1777:1811	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	7	81	theme	SA	1557:1558	arg1	chains					1560:1565	SA chains	1557:1565	SA chains	1557:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	3	82	theme	FDA-approved	484:495	arg1	polycaprolactone					536:551	polycaprolactone	536:551	polycaprolactone (PCL)	536:557	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	82	theme	FDA-approved	484:495	arg1	biomaterials					497:508	two biodegradable FDA-approved biomaterials	466:508	two biodegradable FDA-approved biomaterials	466:508	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	3	82	theme	FDA-approved	484:495	arg1	alginate					518:525	sodium alginate	511:525	sodium alginate (SA)	511:530	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	0	83	theme	calcium-alginate/polycaprolactone/reduced	14:54	arg1	nanohybrid					71:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid hydrogels for skeletal muscle tissue engineering.
35305322	1	84	theme	Graphene	132:139	arg1	oxide					178:182	reduced graphene oxide	161:182	reduced graphene oxide (rGO)	161:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	84	theme	Graphene	132:139	arg1	derivatives					141:151	Graphene derivatives	132:151	Graphene derivatives such as reduced graphene oxide (rGO)	132:188	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	1	84	theme	Graphene	132:139	arg1	components					202:211	components	202:211	components of novel biomaterials for their unique electrical properties	202:272	Graphene derivatives such as reduced graphene oxide (rGO) are used as components of novel biomaterials for their unique electrical properties.
35305322	7	85	theme	SA	1347:1348	arg1	chains					1350:1355	SA chains	1347:1355	SA chains induced by Ca2+ ions (egg-box model)	1347:1392	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	9	86	with	cultures	1726:1733	arg1	myoblasts					1753:1761	C2C12 murine myoblasts	1740:1761	C2C12 murine myoblasts	1740:1761	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	0	87	theme	oxide	65:69	arg1	nanohybrid					71:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid	0:80	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid hydrogels for skeletal muscle tissue engineering.
35305322	3	88	theme	new	408:410	arg1	type					412:415	a new type	406:415	a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker	406:591	This study reports the development of a new type of semi-interpenetrated polymer network based on two biodegradable FDA-approved biomaterials, sodium alginate (SA) and polycaprolactone (PCL), with Ca2+ ions as SA crosslinker.
35305322	6	89	theme	thermal	1108:1114	arg1	degradation					1116:1126	thermal degradation	1108:1126	thermal degradation	1108:1126	The study focuses on the microstructure and the enhancement of physical and biological properties of these advanced materials, including water sorption, surface wettability, thermal behavior and thermal degradation, mechanical properties, electrical conductivity, cell adhesion and myogenic differentiation.
35305322	0	90	theme	skeletal	96:103	arg1	engineering					119:129	skeletal muscle tissue engineering	96:129	skeletal muscle tissue engineering	96:129	Electroactive calcium-alginate/polycaprolactone/reduced graphene oxide nanohybrid hydrogels for skeletal muscle tissue engineering.
35305322	9	91	theme	myoblast	1855:1862	arg1	adhesion					1864:1871	myoblast adhesion	1855:1871	myoblast adhesion	1855:1871	In vitro cultures with C2C12 murine myoblasts revealed that the conductive nanohybrid hydrogels are not cytotoxic and can greatly enhance myoblast adhesion and myogenic differentiation.
35305322	8	92	theme	rGO	1589:1591	arg1	incorporation					1572:1584	The incorporation	1568:1584	The incorporation of rGO	1568:1591	The incorporation of rGO significantly increases the electrical conductivity of the nanohybrid hydrogels, with values in the range of muscle tissue.
35305322	7	93	theme	hydrogen	1513:1520	arg1	bonding					1522:1528	strong hydrogen bonding	1506:1528	strong hydrogen bonding between rGO nanosheets and SA chains	1506:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	7	94	dep	components	1321:1330	arg1	bonds					1333:1337	bonds	1333:1337	the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains	1317:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	7	95	theme	different	1286:1294	arg1	interactions					1296:1307	different interactions	1286:1307	different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains	1286:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	4	96	theme	low	624:626	arg1	adhesion					633:640	the low cell adhesion	620:640	the low cell adhesion of SA and weak structural stability	620:676	Several drawbacks such as the low cell adhesion of SA and weak structural stability can be improved with the incorporation of PCL.
35305322	5	97	theme	wt/wt	831:835	arg1	nanosheets					841:850	wt/wt rGO nanosheets	831:850	wt/wt rGO nanosheets	831:850	Furthermore, this study demonstrates how this semi-IPN can be engineered with rGO nanosheets (0.5% and 2% wt/wt rGO nanosheets) to produce electroactive nanohybrid composite biomaterials.
35305322	7	98	theme	nano-network	1268:1279	arg1	formation					1245:1253	the formation	1241:1253	the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains	1241:1565	The results suggest the formation of a complex nano-network with different interactions between the components: bonds between SA chains induced by Ca2+ ions (egg-box model), links between rGO nanosheets and SA chains as well as between rGO nanosheets themselves through Ca2+ ions, and strong hydrogen bonding between rGO nanosheets and SA chains.
35305322	10	99	theme	tissue	2113:2118	arg1	engineering					2120:2130	tissue engineering	2113:2130	skeletal muscle tissue engineering	2097:2130	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
35305322	8	100	theme	electrical	1621:1630	arg1	conductivity					1632:1643	the electrical conductivity	1617:1643	the electrical conductivity of the nanohybrid hydrogels	1617:1671	The incorporation of rGO significantly increases the electrical conductivity of the nanohybrid hydrogels, with values in the range of muscle tissue.
35305322	10	101	theme	electroactive	1943:1955	arg1	hydrogels					1968:1976	these novel electroactive nanohybrid hydrogels	1931:1976	these novel electroactive nanohybrid hydrogels	1931:1976	These results indicate that these novel electroactive nanohybrid hydrogels have great potential for biomedical applications related to the regeneration of electroactive tissues, particularly in skeletal muscle tissue engineering.
36868069	0	0	theme	fresh-cut	82:90	arg1	apples					92:97	fresh-cut apples	82:97	fresh-cut apples	82:97	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	6	1	theme	cold	1079:1082	arg1	storage					1084:1090	cold storage	1079:1090	cold storage	1079:1090	Overall, this study showed that CNF coating could effectively maintain the quality of fresh-cut apples during cold storage.
36868069	3	2	theme	titratable	612:621	arg1	acid					623:626	titratable acid	612:626	titratable acid	612:626	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	4	3	theme	chromatography-mass	648:666	arg1	spectrometry					668:679	Gas chromatography-mass spectrometry	644:679	Gas chromatography-mass spectrometry	644:679	Gas chromatography-mass spectrometry showed that CNF treatment could maintain the aroma components of apple wedges (stored for 4 days).
36868069	5	4	theme	membrane	918:925	arg1	peroxidation					933:944	membrane lipid peroxidation	918:944	membrane lipid peroxidation level of apple wedges	918:966	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	3	5	theme	apple	504:508	arg1	wedges					510:515	apple wedges	504:515	apple wedges	504:515	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	2	6	theme	CNF	283:285	arg1	treatment					287:295	CNF treatment	283:295	CNF treatment	283:295	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	0	7	theme	apples	92:97	arg1	aroma					73:77	aroma	73:77	aroma of fresh-cut apples	73:97	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	0	7	theme	apples	92:97	arg1	properties					58:67	antioxidant properties	46:67	antioxidant properties	46:67	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	5	8	theme	apple	955:959	arg1	wedges					961:966	apple wedges	955:966	apple wedges	955:966	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	3	9	theme	decay	530:534	arg1	rate					536:539	the decay rate	526:539	the decay rate of apple wedges	526:555	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	3	10	from	decline	574:580	arg1	acid					623:626	titratable acid	612:626	titratable acid	612:626	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	3	10	from	decline	574:580	arg1	firmness					598:605	firmness	598:605	firmness	598:605	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	3	10	from	decline	574:580	arg1	loss					592:595	weight loss	585:595	weight loss	585:595	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	4	11	theme	CNF	693:695	arg1	treatment					697:705	CNF treatment	693:705	CNF treatment	693:705	Gas chromatography-mass spectrometry showed that CNF treatment could maintain the aroma components of apple wedges (stored for 4 days).
36868069	4	12	theme	wedges	752:757	arg1	components					732:741	the aroma components	722:741	the aroma components of apple wedges (stored for 4 days)	722:777	Gas chromatography-mass spectrometry showed that CNF treatment could maintain the aroma components of apple wedges (stored for 4 days).
36868069	5	13	theme	antioxidant	843:853	arg1	level					862:866	the antioxidant system level	839:866	the antioxidant system level	839:866	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	6	14	theme	apples	1065:1070	arg1	quality					1044:1050	the quality	1040:1050	the quality of fresh-cut apples	1040:1070	Overall, this study showed that CNF coating could effectively maintain the quality of fresh-cut apples during cold storage.
36868069	5	15	theme	reactive	882:889	arg1	species					898:904	reactive oxygen species	882:904	reactive oxygen species content	882:912	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	4	16	theme	apple	746:750	arg1	wedges					752:757	apple wedges	746:757	apple wedges (stored for 4 days)	746:777	Gas chromatography-mass spectrometry showed that CNF treatment could maintain the aroma components of apple wedges (stored for 4 days).
36868069	2	17	theme	apple	376:380	arg1	wedges					400:405	fresh-cut apple (Malus domestica) wedges	366:405	fresh-cut apple (Malus domestica) wedges	366:405	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	0	18	from	treatment	33:41	arg1	aroma					73:77	aroma	73:77	aroma of fresh-cut apples	73:97	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	0	18	from	treatment	33:41	arg1	properties					58:67	antioxidant properties	46:67	antioxidant properties	46:67	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	2	19	theme	storage	304:310	arg1	quality					312:318	storage quality	304:318	storage quality	304:318	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	20	theme	fresh-cut	366:374	arg1	apple					376:380	fresh-cut apple	366:380	fresh-cut apple (Malus domestica) wedges	366:405	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	20	theme	fresh-cut	366:374	arg1	domestica					389:397	Malus domestica	383:397	Malus domestica	383:397	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	21	dep	quality	312:318	arg1	the					300:302	the	300:302	the	300:302	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	5	22	theme	system	855:860	arg1	level					862:866	the antioxidant system level	839:866	the antioxidant system level	839:866	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	0	23	theme	nanofibrils	21:31	arg1	Effects					0:6	Effects	0:6	Effects of cellulose nanofibrils	0:31	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	6	24	theme	fresh-cut	1055:1063	arg1	apples					1065:1070	fresh-cut apples	1055:1070	fresh-cut apples	1055:1070	Overall, this study showed that CNF coating could effectively maintain the quality of fresh-cut apples during cold storage.
36868069	1	25	theme	Horticultural	100:112	arg1	products					114:121	Horticultural products	100:121	Horticultural products	100:121	Horticultural products tend to deteriorate during postharvest storage and processing.
36868069	2	26	theme	wedges	400:405	arg1	quality					312:318	storage quality	304:318	storage quality	304:318	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	26	theme	wedges	400:405	arg1	composition					327:337	aroma composition	321:337	aroma composition	321:337	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	26	theme	wedges	400:405	arg1	system					356:361	antioxidant system	344:361	antioxidant system	344:361	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	0	27	theme	cellulose	11:19	arg1	nanofibrils					21:31	cellulose nanofibrils	11:31	cellulose nanofibrils	11:31	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	5	28	theme	lipid	927:931	arg1	peroxidation					933:944	membrane lipid peroxidation	918:944	membrane lipid peroxidation level of apple wedges	918:966	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	5	29	theme	peroxidation	933:944	arg1	level					946:950	membrane lipid peroxidation level	918:950	membrane lipid peroxidation level of apple wedges	918:966	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	2	30	theme	treatment	287:295	arg1	effects					272:278	the effects	268:278	the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges	268:405	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	3	31	theme	coating	445:451	arg1	treatment					453:461	CNF coating treatment	441:461	CNF coating treatment	441:461	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	5	32	theme	species	898:904	arg1	content					906:912	reactive oxygen species content	882:912	reactive oxygen species content	882:912	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	5	33	theme	CNF	815:817	arg1	treatment					819:827	CNF treatment	815:827	CNF treatment	815:827	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	3	34	theme	wedges	550:555	arg1	rate					536:539	the decay rate	526:539	the decay rate of apple wedges	526:555	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	4	35	theme	Gas	644:646	arg1	spectrometry					668:679	Gas chromatography-mass spectrometry	644:679	Gas chromatography-mass spectrometry	644:679	Gas chromatography-mass spectrometry showed that CNF treatment could maintain the aroma components of apple wedges (stored for 4 days).
36868069	5	36	theme	Further	780:786	arg1	investigations					788:801	Further investigations	780:801	Further investigations	780:801	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	2	37	theme	Malus	383:387	arg1	apple					376:380	fresh-cut apple	366:380	fresh-cut apple (Malus domestica) wedges	366:405	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	37	theme	Malus	383:387	arg1	domestica					389:397	Malus domestica	383:397	Malus domestica	383:397	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	5	38	theme	oxygen	891:896	arg1	species					898:904	reactive oxygen species	882:904	reactive oxygen species content	882:912	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	0	39	theme	antioxidant	46:56	arg1	properties					58:67	antioxidant properties	46:67	antioxidant properties	46:67	Effects of cellulose nanofibrils treatment on antioxidant properties and aroma of fresh-cut apples.
36868069	2	40	theme	aroma	321:325	arg1	composition					327:337	aroma composition	321:337	aroma composition	321:337	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	41	from	effects	272:278	arg1	quality					312:318	storage quality	304:318	storage quality	304:318	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	41	from	effects	272:278	arg1	composition					327:337	aroma composition	321:337	aroma composition	321:337	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	41	from	effects	272:278	arg1	system					356:361	antioxidant system	344:361	antioxidant system	344:361	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	3	42	theme	apple	544:548	arg1	wedges					550:555	apple wedges	544:555	apple wedges	544:555	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	3	43	theme	weight	585:590	arg1	loss					592:595	weight loss	585:595	weight loss	585:595	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	4	44	theme	aroma	726:730	arg1	components					732:741	the aroma components	722:741	the aroma components of apple wedges (stored for 4 days)	722:777	Gas chromatography-mass spectrometry showed that CNF treatment could maintain the aroma components of apple wedges (stored for 4 days).
36868069	2	45	theme	cellulose	201:209	arg1	CNFs					223:226	CNFs	223:226	CNFs	223:226	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	2	45	theme	cellulose	201:209	arg1	nanofibers					211:220	cellulose nanofibers	201:220	cellulose nanofibers (CNFs)	201:227	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
36868069	3	46	theme	wedges	510:515	arg1	appearance					490:499	the appearance	486:499	the appearance of apple wedges	486:515	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	6	47	theme	CNF	1001:1003	arg1	coating					1005:1011	CNF coating	1001:1011	CNF coating	1001:1011	Overall, this study showed that CNF coating could effectively maintain the quality of fresh-cut apples during cold storage.
36868069	5	48	theme	wedges	961:966	arg1	level					946:950	membrane lipid peroxidation level	918:950	membrane lipid peroxidation level of apple wedges	918:966	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	5	48	theme	wedges	961:966	arg1	content					906:912	reactive oxygen species content	882:912	reactive oxygen species content	882:912	Further investigations showed that CNF treatment increased the antioxidant system level and decreased reactive oxygen species content and membrane lipid peroxidation level of apple wedges.
36868069	3	49	theme	CNF	441:443	arg1	treatment					453:461	CNF coating treatment	441:461	CNF coating treatment	441:461	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	1	50	theme	postharvest	150:160	arg1	storage					162:168	postharvest storage	150:168	postharvest storage	150:168	Horticultural products tend to deteriorate during postharvest storage and processing.
36868069	3	51	theme	control	422:428	arg1	treatment					430:438	control treatment	422:438	control treatment	422:438	Compared with control treatment, CNF coating treatment significantly improved the appearance of apple wedges; reduced the decay rate of apple wedges; and delayed the decline in weight loss, firmness, and titratable acid during storage.
36868069	2	52	theme	antioxidant	344:354	arg1	system					356:361	antioxidant system	344:361	antioxidant system	344:361	In this study, cellulose nanofibers (CNFs) were prepared from wood to investigate the effects of CNF treatment on the storage quality, aroma composition, and antioxidant system of fresh-cut apple (Malus domestica) wedges.
35961554	0	0	theme	experimental	93:104	arg1	injury					122:127	experimental traumatic brain injury	93:127	experimental traumatic brain injury	93:127	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	6	1	theme	biocompatible	878:890	arg1	features					892:899	well biocompatible features	873:899	well biocompatible features	873:899	Moreover, GGA/Odex hydrogel possessed well biocompatible features.
35961554	4	2	theme	dextran	675:681	arg1	reaction					613:620	the Schiff base reaction	597:620	the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex)	597:688	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	5	3	theme	DPPH	750:753	arg1	radicals					764:771	DPPH and ABTS radicals	750:771	DPPH and ABTS radicals	750:771	The resulting GGA/Odex hydrogel could effectively scavenge DPPH and ABTS radicals, as well as protect cells from the oxidative damage in vitro.
35961554	7	4	theme	memory	1049:1054	arg1	abilities					1056:1064	the motor, learning and memory abilities	1025:1064	the motor, learning and memory abilities	1025:1064	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	1	5	theme	reactive	179:186	arg1	ROS					204:206	ROS	204:206	ROS	204:206	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	1	5	theme	reactive	179:186	arg1	species					195:201	reactive oxygen species	179:201	reactive oxygen species (ROS)	179:207	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	1	6	theme	traumatic	256:264	arg1	TBI					280:282	TBI	280:282	TBI	280:282	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	1	6	theme	traumatic	256:264	arg1	injury					272:277	traumatic brain injury	256:277	traumatic brain injury (TBI)	256:283	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	0	7	theme	brain	116:120	arg1	injury					122:127	experimental traumatic brain injury	93:127	experimental traumatic brain injury	93:127	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	7	8	theme	moderate	907:914	arg1	model					926:930	a moderate TBI mouse model	905:930	a moderate TBI mouse model	905:930	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	4	9	theme	gelatin	648:654	arg1	reaction					613:620	the Schiff base reaction	597:620	the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex)	597:688	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	1	10	theme	brain	266:270	arg1	TBI					280:282	TBI	280:282	TBI	280:282	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	1	10	theme	brain	266:270	arg1	injury					272:277	traumatic brain injury	256:277	traumatic brain injury (TBI)	256:283	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	0	11	theme	traumatic	106:114	arg1	injury					122:127	experimental traumatic brain injury	93:127	experimental traumatic brain injury	93:127	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	9	12	theme	GGA6Odex	1320:1327	arg1	hydrogel					1329:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	9	12	theme	GGA6Odex	1320:1327	arg1	biomaterial					1353:1363	a promising biomaterial	1341:1363	a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance	1341:1471	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	9	13	theme	promising	1343:1351	arg1	hydrogel					1329:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	9	13	theme	promising	1343:1351	arg1	biomaterial					1353:1363	a promising biomaterial	1341:1363	a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance	1341:1471	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	7	14	theme	TBI	916:918	arg1	model					926:930	a moderate TBI mouse model	905:930	a moderate TBI mouse model	905:930	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	1	15	theme	injury	272:277	arg1	pathogenesis					240:251	the pathogenesis	236:251	the pathogenesis of traumatic brain injury (TBI)	236:283	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	4	16	theme	oxidized	666:673	arg1	Odex					684:687	Odex	684:687	Odex	684:687	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	4	16	theme	oxidized	666:673	arg1	dextran					675:681	oxidized dextran	666:681	oxidized dextran (Odex)	666:688	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	3	17	theme	great	471:475	arg1	significance					477:488	great significance	471:488	great significance	471:488	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	7	18	theme	mouse	920:924	arg1	model					926:930	a moderate TBI mouse model	905:930	a moderate TBI mouse model	905:930	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	3	19	with	design	390:395	arg1	ability					457:463	ROS scavenging ability	442:463	ROS scavenging ability	442:463	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	1	20	theme	oxygen	188:193	arg1	ROS					204:206	ROS	204:206	ROS	204:206	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	1	20	theme	oxygen	188:193	arg1	species					195:201	reactive oxygen species	179:201	reactive oxygen species (ROS)	179:207	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	9	21	theme	raised	1449:1454	arg1	circumstance					1460:1471	raised ROS circumstance	1449:1471	raised ROS circumstance	1449:1471	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	8	22	dep	factors	1217:1223	arg1	factors					1217:1223	inflammatory factors secretion and macrophage/microglia polarization	1204:1271	inflammatory factors secretion and macrophage/microglia polarization	1204:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	22	dep	factors	1217:1223	arg1	secretion					1225:1233	secretion	1225:1233	secretion	1225:1233	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	22	dep	factors	1217:1223	arg1	polarization					1260:1271	macrophage/microglia polarization	1239:1271	macrophage/microglia polarization	1239:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	1	23	theme	species	195:201	arg1	overexpression					161:174	the overexpression	157:174	the overexpression of reactive oxygen species (ROS)	157:207	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	8	24	theme	Nrf2/HO-1	1164:1172	arg1	pathway					1174:1180	Nrf2/HO-1 pathway	1164:1180	Nrf2/HO-1 pathway	1164:1180	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	3	25	dep	design	390:395	arg1	the					386:388	the	386:388	the	386:388	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	4	26	theme	acid-conjugated	632:646	arg1	gelatin					648:654	gallic acid-conjugated gelatin	625:654	gallic acid-conjugated gelatin (GGA)	625:660	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	4	26	theme	acid-conjugated	632:646	arg1	GGA					657:659	GGA	657:659	GGA	657:659	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	6	27	theme	GGA/Odex	845:852	arg1	hydrogel					854:861	GGA/Odex hydrogel	845:861	GGA/Odex hydrogel	845:861	Moreover, GGA/Odex hydrogel possessed well biocompatible features.
35961554	8	28	theme	macrophage/microglia	1239:1258	arg1	factors					1217:1223	inflammatory factors secretion and macrophage/microglia polarization	1204:1271	inflammatory factors secretion and macrophage/microglia polarization	1204:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	28	theme	macrophage/microglia	1239:1258	arg1	polarization					1260:1271	macrophage/microglia polarization	1239:1271	macrophage/microglia polarization	1239:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	0	29	theme	Injectable	0:9	arg1	hydrogel					58:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	4	30	theme	antioxidant	535:545	arg1	hydrogel					547:554	an injectable and antioxidant hydrogel	517:554	an injectable and antioxidant hydrogel	517:554	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	6	31	contain	possessed	863:871	arg2	features					892:899	well biocompatible features	873:899	well biocompatible features	873:899	Moreover, GGA/Odex hydrogel possessed well biocompatible features.
35961554	6	31	contain	possessed	863:871	arg1	hydrogel					854:861	GGA/Odex hydrogel	845:861	GGA/Odex hydrogel	845:861	Moreover, GGA/Odex hydrogel possessed well biocompatible features.
35961554	2	32	theme	ROS	302:304	arg1	Accumulation					286:297	Accumulation	286:297	Accumulation of ROS	286:304	Accumulation of ROS can lead to cell death, neurodegeneration, and neurological deficit.
35961554	7	33	dep	in	933:934	arg1	situ					936:939	situ	936:939	situ	936:939	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	2	34	theme	neurological	353:364	arg1	deficit					366:372	neurological deficit	353:372	neurological deficit	353:372	Accumulation of ROS can lead to cell death, neurodegeneration, and neurological deficit.
35961554	0	35	theme	oxygen	24:29	arg1	hydrogel					58:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	7	36	theme	learning	1036:1043	arg1	abilities					1056:1064	the motor, learning and memory abilities	1025:1064	the motor, learning and memory abilities	1025:1064	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	9	37	theme	ROS	1456:1458	arg1	circumstance					1460:1471	raised ROS circumstance	1449:1471	raised ROS circumstance	1449:1471	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	3	38	theme	scavenging	446:455	arg1	ability					457:463	ROS scavenging ability	442:463	ROS scavenging ability	442:463	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	0	39	theme	reactive	15:22	arg1	hydrogel					58:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	3	40	theme	neural	494:499	arg1	repair					501:506	neural repair	494:506	neural repair	494:506	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	4	41	theme	base	608:611	arg1	reaction					613:620	the Schiff base reaction	597:620	the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex)	597:688	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	0	42	theme	gelatin	50:56	arg1	hydrogel					58:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	9	43	theme	tissue	1369:1374	arg1	medicine					1389:1396	tissue regenerative medicine	1369:1396	tissue regenerative medicine	1369:1396	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	7	44	theme	motor	1029:1033	arg1	abilities					1056:1064	the motor, learning and memory abilities	1025:1064	the motor, learning and memory abilities	1025:1064	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	9	45	theme	injectable	1290:1299	arg1	hydrogel					1329:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	9	45	theme	injectable	1290:1299	arg1	biomaterial					1353:1363	a promising biomaterial	1341:1363	a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance	1341:1471	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	4	46	theme	gallic	625:630	arg1	gelatin					648:654	gallic acid-conjugated gelatin	625:654	gallic acid-conjugated gelatin (GGA)	625:660	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	4	46	theme	gallic	625:630	arg1	GGA					657:659	GGA	657:659	GGA	657:659	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	0	47	theme	species-scavenging	31:48	arg1	hydrogel					58:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel	0:65	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	9	48	theme	regenerative	1376:1387	arg1	medicine					1389:1396	tissue regenerative medicine	1369:1396	tissue regenerative medicine	1369:1396	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	5	49	theme	ABTS	759:762	arg1	radicals					764:771	DPPH and ABTS radicals	750:771	DPPH and ABTS radicals	750:771	The resulting GGA/Odex hydrogel could effectively scavenge DPPH and ABTS radicals, as well as protect cells from the oxidative damage in vitro.
35961554	9	50	theme	other	1417:1421	arg1	repair					1430:1435	other tissue repair	1417:1435	other tissue repair	1417:1435	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	5	51	theme	oxidative	808:816	arg1	damage					818:823	the oxidative damage	804:823	the oxidative damage	804:823	The resulting GGA/Odex hydrogel could effectively scavenge DPPH and ABTS radicals, as well as protect cells from the oxidative damage in vitro.
35961554	3	52	theme	ROS	442:444	arg1	ability					457:463	ROS scavenging ability	442:463	ROS scavenging ability	442:463	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	8	53	theme	inflammatory	1204:1215	arg1	factors					1217:1223	inflammatory factors secretion and macrophage/microglia polarization	1204:1271	inflammatory factors secretion and macrophage/microglia polarization	1204:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	53	theme	inflammatory	1204:1215	arg1	secretion					1225:1233	secretion	1225:1233	secretion	1225:1233	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	53	theme	inflammatory	1204:1215	arg1	polarization					1260:1271	macrophage/microglia polarization	1239:1271	macrophage/microglia polarization	1239:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	3	54	with	application	401:411	arg1	ability					457:463	ROS scavenging ability	442:463	ROS scavenging ability	442:463	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	3	55	theme	functional	416:425	arg1	materials					427:435	functional materials	416:435	functional materials	416:435	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	1	56	theme	Oxidative	130:138	arg1	stress					140:145	Oxidative stress	130:145	Oxidative stress caused by the overexpression of reactive oxygen species (ROS)	130:207	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	9	57	theme	ROS-scavenging	1305:1318	arg1	hydrogel					1329:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	this injectable and ROS-scavenging GGA6Odex hydrogel	1285:1336	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	9	57	theme	ROS-scavenging	1305:1318	arg1	biomaterial					1353:1363	a promising biomaterial	1341:1363	a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance	1341:1471	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	4	58	theme	injectable	520:529	arg1	hydrogel					547:554	an injectable and antioxidant hydrogel	517:554	an injectable and antioxidant hydrogel	517:554	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	7	59	theme	GGA6Odex	957:964	arg1	hydrogel					966:973	GGA6Odex hydrogel	957:973	GGA6Odex hydrogel	957:973	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	2	60	theme	cell	318:321	arg1	death					323:327	cell death	318:327	cell death	318:327	Accumulation of ROS can lead to cell death, neurodegeneration, and neurological deficit.
35961554	7	61	theme	hydrogel	966:973	arg1	implantation					941:952	in situ implantation	933:952	in situ implantation of GGA6Odex hydrogel	933:973	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	5	62	theme	GGA/Odex	705:712	arg1	hydrogel					714:721	The resulting GGA/Odex hydrogel	691:721	The resulting GGA/Odex hydrogel	691:721	The resulting GGA/Odex hydrogel could effectively scavenge DPPH and ABTS radicals, as well as protect cells from the oxidative damage in vitro.
35961554	8	63	theme	composite	1078:1086	arg1	hydrogel					1088:1095	this composite hydrogel	1073:1095	this composite hydrogel	1073:1095	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	9	64	theme	relevant	1437:1444	arg1	TBI					1409:1411	TBI	1409:1411	TBI	1409:1411	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	8	65	theme	oxidative	1108:1116	arg1	stress					1118:1123	oxidative stress	1108:1123	oxidative stress	1108:1123	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	1	66	theme	important	218:226	arg1	role					228:231	an important role	215:231	an important role	215:231	Oxidative stress caused by the overexpression of reactive oxygen species (ROS) plays an important role in the pathogenesis of traumatic brain injury (TBI).
35961554	3	67	theme	materials	427:435	arg1	application					401:411	application	401:411	application	401:411	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	3	67	theme	materials	427:435	arg1	design					390:395	design	390:395	design	390:395	Therefore, the design and application of functional materials with ROS scavenging ability is of great significance for neural repair.
35961554	4	68	theme	Schiff	601:606	arg1	reaction					613:620	the Schiff base reaction	597:620	the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex)	597:688	Herein, an injectable and antioxidant hydrogel was developed for TBI treatment based on the Schiff base reaction of gallic acid-conjugated gelatin (GGA) and oxidized dextran (Odex).
35961554	0	69	theme	neural	76:81	arg1	repair					83:88	neural repair	76:88	neural repair	76:88	Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.
35961554	8	70	theme	factors	1217:1223	arg1	regulating					1190:1199	the regulating	1186:1199	the regulating of inflammatory factors secretion and macrophage/microglia polarization	1186:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	70	theme	factors	1217:1223	arg1	activation					1150:1159	the activation	1146:1159	the activation of Nrf2/HO-1 pathway	1146:1180	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	71	theme	pathway	1174:1180	arg1	regulating					1190:1199	the regulating	1186:1199	the regulating of inflammatory factors secretion and macrophage/microglia polarization	1186:1271	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	8	71	theme	pathway	1174:1180	arg1	activation					1150:1159	the activation	1146:1159	the activation of Nrf2/HO-1 pathway	1146:1180	Also, this composite hydrogel suppressed oxidative stress and inflammation via the activation of Nrf2/HO-1 pathway and the regulating of inflammatory factors secretion and macrophage/microglia polarization.
35961554	7	72	theme	in	933:934	arg1	implantation					941:952	in situ implantation	933:952	in situ implantation of GGA6Odex hydrogel	933:973	In a moderate TBI mouse model, in situ implantation of GGA6Odex hydrogel efficiently facilitated neurogenesis and promoted the motor, learning and memory abilities.
35961554	9	73	theme	tissue	1423:1428	arg1	repair					1430:1435	other tissue repair	1417:1435	other tissue repair	1417:1435	Therefore, this injectable and ROS-scavenging GGA6Odex hydrogel is a promising biomaterial for tissue regenerative medicine, including TBI and other tissue repair relevant to raised ROS circumstance.
35961554	5	74	theme	resulting	695:703	arg1	hydrogel					714:721	The resulting GGA/Odex hydrogel	691:721	The resulting GGA/Odex hydrogel	691:721	The resulting GGA/Odex hydrogel could effectively scavenge DPPH and ABTS radicals, as well as protect cells from the oxidative damage in vitro.
35380849	6	0	theme	Successful	935:944	arg1	targeting					955:963	Successful in vitro targeting	935:963	Successful in vitro targeting of breast cancer cells	935:986	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	6	1	theme	cells	982:986	arg1	targeting					955:963	Successful in vitro targeting	935:963	Successful in vitro targeting of breast cancer cells	935:986	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	7	2	theme	Pt	1292:1293	arg1	sera					1295:1298	BrCr Pt sera	1287:1298	BrCr Pt sera	1287:1298	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	5	3	theme	breast	817:822	arg1	Pt					846:847	BrCr Pt	841:847	BrCr Pt	841:847	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	3	theme	breast	817:822	arg1	patients					831:838	breast cancer patients	817:838	breast cancer patients (BrCr Pt)	817:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	4	theme	proteomic	696:704	arg1	analysis					706:713	proteomic analysis	696:713	proteomic analysis	696:713	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	8	5	theme	serum	1342:1346	arg1	source					1348:1353	the serum source	1338:1353	the serum source	1338:1353	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	5	6	from	volunteers	801:810	arg1	composition					757:767	the composition	753:767	the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt)	753:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	6	from	volunteers	801:810	arg1	Pt					846:847	BrCr Pt	841:847	BrCr Pt	841:847	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	6	from	volunteers	801:810	arg1	sera					777:780	sera	777:780	sera from healthy human volunteers	777:810	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	6	from	volunteers	801:810	arg1	FBS					772:774	FBS	772:774	FBS	772:774	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	6	from	volunteers	801:810	arg1	patients					831:838	breast cancer patients	817:838	breast cancer patients (BrCr Pt)	817:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	6	7	theme	cancer	975:980	arg1	cells					982:986	breast cancer cells	968:986	breast cancer cells	968:986	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	1	8	from	Impact	47:52	arg1	Targeting					86:94	Cellular Targeting	77:94	Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles	77:146	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	4	9	theme	corona	528:533	arg1	role					516:519	the role	512:519	the role of the corona	512:533	In the former, the role of the corona is overlooked, and in the latter, the effects of a corona that does not represent the one forming in humans nor the respective disease state are considered.
35380849	7	10	theme	specific	1202:1209	arg1	proteins					1217:1224	specific serum proteins	1202:1224	specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera	1202:1298	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	8	11	theme	limited	1546:1552	arg1	success					1563:1569	the limited clinical success	1542:1569	the limited clinical success of drug targeting by NPs in cancer	1542:1604	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	7	12	theme	serum	1211:1215	arg1	proteins					1217:1224	specific serum proteins	1202:1224	specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera	1202:1298	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	2	13	theme	biological	290:299	arg1	fate					301:304	their cellular interaction and biological fate	259:304	fate	301:304	The nanoparticle (NP) protein corona represents an interface between biological components and NPs, dictating their cellular interaction and biological fate.
35380849	4	14	theme	corona	586:591	arg1	effects					573:579	the effects	569:579	the effects of a corona that does not represent the one forming in humans	569:641	In the former, the role of the corona is overlooked, and in the latter, the effects of a corona that does not represent the one forming in humans nor the respective disease state are considered.
35380849	8	15	theme	targeting	1482:1490	arg1	success					1453:1459	consequent success	1442:1459	consequent success	1442:1459	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	8	15	theme	targeting	1482:1490	arg1	failure					1464:1470	failure	1464:1470	failure	1464:1470	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	8	15	theme	targeting	1482:1490	arg1	reliability					1367:1377	the reliability	1363:1377	the reliability of in vitro experiments assessing NP-cell interactions	1363:1432	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	4	16	theme	respective	651:660	arg1	state					670:674	the respective disease state	647:674	the respective disease state	647:674	In the former, the role of the corona is overlooked, and in the latter, the effects of a corona that does not represent the one forming in humans nor the respective disease state are considered.
35380849	2	17	theme	nanoparticle	153:164	arg1	corona					179:184	The nanoparticle (NP) protein corona	149:184	The nanoparticle (NP) protein corona	149:184	The nanoparticle (NP) protein corona represents an interface between biological components and NPs, dictating their cellular interaction and biological fate.
35380849	3	18	theme	fetal	471:475	arg1	FBS					491:493	FBS	491:493	FBS	491:493	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	3	18	theme	fetal	471:475	arg1	serum					484:488	fetal bovine serum	471:488	fetal bovine serum (FBS)	471:494	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	3	19	theme	targeting	341:349	arg1	success					321:327	the success	317:327	the success of cellular targeting	317:349	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	5	20	from	difference	739:748	arg1	composition					757:767	the composition	753:767	the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt)	753:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	6	21	theme	breast	968:973	arg1	cells					982:986	breast cancer cells	968:986	breast cancer cells	968:986	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	6	22	dep	in	946:947	arg1	vitro					949:953	vitro	949:953	vitro	949:953	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	8	23	from	NPs	1592:1594	arg1	cancer					1599:1604	cancer	1599:1604	cancer	1599:1604	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	3	24	theme	culture	434:440	arg1	medium					442:447	serum-free culture medium	423:447	serum-free culture medium	423:447	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	8	25	theme	active	1475:1480	arg1	targeting					1482:1490	active targeting	1475:1490	active targeting	1475:1490	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	0	26	theme	Corona	35:40	arg1	!					41:41	Different Serum, Different Protein Corona!	0:41	Different Serum, Different Protein Corona!	0:41	Different Serum, Different Protein Corona!
35380849	1	27	theme	Serum	61:65	arg1	Source					67:72	the Serum Source	57:72	the Serum Source	57:72	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	8	28	theme	clinical	1554:1561	arg1	success					1563:1569	the limited clinical success	1542:1569	the limited clinical success of drug targeting by NPs in cancer	1542:1604	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	0	29	theme	Serum	10:14	arg1	!					41:41	Different Serum, Different Protein Corona!	0:41	Different Serum, Different Protein Corona!	0:41	Different Serum, Different Protein Corona!
35380849	7	30	theme	BrCr	1287:1290	arg1	sera					1295:1298	BrCr Pt sera	1287:1298	BrCr Pt sera	1287:1298	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	8	31	dep	success	1453:1459	arg1	the					1438:1440	the	1438:1440	the	1438:1440	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	1	32	theme	Source	67:72	arg1	Impact					47:52	The Impact	43:52	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles	43:146	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	7	33	theme	sera	1295:1298	arg1	presence					1275:1282	the presence	1271:1282	the presence of BrCr Pt sera	1271:1298	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	5	34	theme	sera	777:780	arg1	composition					757:767	the composition	753:767	the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt)	753:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	6	35	theme	Pt	1072:1073	arg1	sera					1075:1078	BrCr Pt sera	1067:1078	BrCr Pt sera	1067:1078	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	8	36	theme	in	1382:1383	arg1	experiments					1391:1401	in vitro experiments	1382:1401	in vitro experiments assessing NP-cell interactions	1382:1432	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	5	37	theme	FBS	772:774	arg1	composition					757:767	the composition	753:767	the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt)	753:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	38	theme	BrCr	841:844	arg1	Pt					846:847	BrCr Pt	841:847	BrCr Pt	841:847	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	38	theme	BrCr	841:844	arg1	patients					831:838	breast cancer patients	817:838	breast cancer patients (BrCr Pt)	817:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	3	39	theme	serum-free	423:432	arg1	medium					442:447	serum-free culture medium	423:447	serum-free culture medium	423:447	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	5	40	theme	different	889:897	arg1	coronas					907:913	completely different protein coronas	878:913	completely different protein coronas around the same NP	878:932	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	41	theme	patients	831:838	arg1	composition					757:767	the composition	753:767	the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt)	753:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	1	42	theme	Cellular	77:84	arg1	Targeting					86:94	Cellular Targeting	77:94	Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles	77:146	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	6	43	theme	in	946:947	arg1	targeting					955:963	Successful in vitro targeting	935:963	Successful in vitro targeting of breast cancer cells	935:986	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	3	44	theme	targeting	370:378	arg1	ligands					380:386	targeting ligands	370:386	targeting ligands	370:386	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	8	45	theme	experiments	1391:1401	arg1	success					1453:1459	consequent success	1442:1459	consequent success	1442:1459	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	8	45	theme	experiments	1391:1401	arg1	failure					1464:1470	failure	1464:1470	failure	1464:1470	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	8	45	theme	experiments	1391:1401	arg1	reliability					1367:1377	the reliability	1363:1377	the reliability of in vitro experiments assessing NP-cell interactions	1363:1432	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	2	46	theme	protein	171:177	arg1	corona					179:184	The nanoparticle (NP) protein corona	149:184	The nanoparticle (NP) protein corona	149:184	The nanoparticle (NP) protein corona represents an interface between biological components and NPs, dictating their cellular interaction and biological fate.
35380849	4	47	theme	disease	662:668	arg1	state					670:674	the respective disease state	647:674	the respective disease state	647:674	In the former, the role of the corona is overlooked, and in the latter, the effects of a corona that does not represent the one forming in humans nor the respective disease state are considered.
35380849	8	48	theme	targeting	1579:1587	arg1	success					1563:1569	the limited clinical success	1542:1569	the limited clinical success of drug targeting by NPs in cancer	1542:1604	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	5	49	theme	healthy	787:793	arg1	volunteers					801:810	healthy human volunteers	787:810	healthy human volunteers	787:810	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	3	50	theme	target	407:412	arg1	cells					414:418	target cells	407:418	target cells	407:418	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	3	51	theme	serum	484:488	arg1	presence					459:466	the presence	455:466	the presence of fetal bovine serum (FBS)	455:494	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	5	52	theme	coronas	907:913	arg1	formation					865:873	the formation	861:873	the formation of completely different protein coronas around the same NP	861:932	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	8	53	theme	drug	1574:1577	arg1	targeting					1579:1587	drug targeting	1574:1587	drug targeting by NPs in cancer	1574:1604	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	0	54	theme	Protein	27:33	arg1	!					41:41	Different Serum, Different Protein Corona!	0:41	Different Serum, Different Protein Corona!	0:41	Different Serum, Different Protein Corona!
35380849	8	55	theme	NP-cell	1413:1419	arg1	interactions					1421:1432	NP-cell interactions	1413:1432	NP-cell interactions	1413:1432	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	5	56	from	composition	757:767	arg1	volunteers					801:810	healthy human volunteers	787:810	healthy human volunteers	787:810	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	2	57	theme	biological	218:227	arg1	components					229:238	biological components	218:238	biological components	218:238	The nanoparticle (NP) protein corona represents an interface between biological components and NPs, dictating their cellular interaction and biological fate.
35380849	3	58	theme	bovine	477:482	arg1	FBS					491:493	FBS	491:493	FBS	491:493	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	3	58	theme	bovine	477:482	arg1	serum					484:488	fetal bovine serum	471:488	fetal bovine serum (FBS)	471:494	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	5	59	theme	same	926:929	arg1	NP					931:932	the same NP	922:932	the same NP	922:932	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	1	60	theme	Folic	99:103	arg1	Nanoparticles					134:146	Folic Acid-Modified Chitosan-Based Nanoparticles	99:146	Folic Acid-Modified Chitosan-Based Nanoparticles	99:146	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	7	61	theme	proteins	1217:1224	arg1	adsorption					1188:1197	the adsorption	1184:1197	the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera	1184:1298	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	6	62	theme	BrCr	1067:1070	arg1	sera					1075:1078	BrCr Pt sera	1067:1078	BrCr Pt sera	1067:1078	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	1	63	theme	Acid-Modified	105:117	arg1	Nanoparticles					134:146	Folic Acid-Modified Chitosan-Based Nanoparticles	99:146	Folic Acid-Modified Chitosan-Based Nanoparticles	99:146	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	7	64	theme	NP	1242:1243	arg1	uptake					1245:1250	NP uptake	1242:1250	NP uptake by cancer cells	1242:1266	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	8	65	theme	consequent	1442:1451	arg1	success					1453:1459	consequent success	1442:1459	consequent success	1442:1459	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	2	66	theme	cellular	265:272	arg1	interaction					274:284	their cellular interaction and biological fate	259:304	interaction	274:284	The nanoparticle (NP) protein corona represents an interface between biological components and NPs, dictating their cellular interaction and biological fate.
35380849	8	67	dep	in	1382:1383	arg1	vitro					1385:1389	vitro	1385:1389	vitro	1385:1389	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	6	68	theme	sera	1075:1078	arg1	presence					1055:1062	the presence	1051:1062	the presence of BrCr Pt sera	1051:1078	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	7	69	theme	cancer	1255:1260	arg1	cells					1262:1266	cancer cells	1255:1266	cancer cells	1255:1266	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	1	70	theme	Chitosan-Based	119:132	arg1	Nanoparticles					134:146	Folic Acid-Modified Chitosan-Based Nanoparticles	99:146	Folic Acid-Modified Chitosan-Based Nanoparticles	99:146	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	5	71	theme	cancer	824:829	arg1	Pt					846:847	BrCr Pt	841:847	BrCr Pt	841:847	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	5	71	theme	cancer	824:829	arg1	patients					831:838	breast cancer patients	817:838	breast cancer patients (BrCr Pt)	817:848	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	7	72	theme	such	1089:1092	arg1	cases					1094:1098	such cases	1089:1098	such cases	1089:1098	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	8	73	theme	additional	1520:1529	arg1	reason					1531:1536	an additional reason	1517:1536	an additional reason for the limited clinical success of drug targeting by NPs in cancer	1517:1604	This work therefore demonstrates how the serum source affects the reliability of in vitro experiments assessing NP-cell interactions and the consequent success or failure of active targeting and may in fact indicate an additional reason for the limited clinical success of drug targeting by NPs in cancer.
35380849	0	74	dep	Serum	10:14	arg1	Different					0:8	Different	0:8	Different	0:8	Different Serum, Different Protein Corona!
35380849	0	74	dep	Serum	10:14	arg1	Different					17:25	Different	17:25	Different	17:25	Different Serum, Different Protein Corona!
35380849	7	75	theme	targeting	1152:1160	arg1	ligand					1162:1167	the targeting ligand itself	1148:1174	the targeting ligand itself	1148:1174	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	1	76	theme	Nanoparticles	134:146	arg1	Targeting					86:94	Cellular Targeting	77:94	Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles	77:146	The Impact of the Serum Source on Cellular Targeting of Folic Acid-Modified Chitosan-Based Nanoparticles.
35380849	6	77	theme	target	1035:1040	arg1	cells					1042:1046	target cells	1035:1046	target cells	1035:1046	Successful in vitro targeting of breast cancer cells was only observed when NPs were incubated with target cells in the presence of BrCr Pt sera only.
35380849	3	78	theme	cellular	332:339	arg1	targeting					341:349	cellular targeting	332:349	cellular targeting	332:349	To assess the success of cellular targeting, NPs modified with targeting ligands are incubated with target cells in serum-free culture medium or in the presence of fetal bovine serum (FBS).
35380849	5	79	theme	protein	899:905	arg1	coronas					907:913	completely different protein coronas	878:913	completely different protein coronas around the same NP	878:932	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	7	80	theme	targeting	1116:1124	arg1	success					1105:1111	the success	1101:1111	the success of targeting	1101:1124	In such cases, the success of targeting was not attributed to the targeting ligand itself, but to the adsorption of specific serum proteins that facilitate NP uptake by cancer cells in the presence of BrCr Pt sera.
35380849	5	81	theme	human	795:799	arg1	volunteers					801:810	healthy human volunteers	787:810	healthy human volunteers	787:810	Via proteomic analysis, we demonstrate how the difference in the composition of FBS, sera from healthy human volunteers, and breast cancer patients (BrCr Pt) results in the formation of completely different protein coronas around the same NP.
35380849	4	82	from	one	621:623	arg1	humans					636:641	humans	636:641	humans	636:641	In the former, the role of the corona is overlooked, and in the latter, the effects of a corona that does not represent the one forming in humans nor the respective disease state are considered.
35380849	4	83	theme	forming	625:631	arg1	one					621:623	the one forming	617:631	the one forming in humans	617:641	In the former, the role of the corona is overlooked, and in the latter, the effects of a corona that does not represent the one forming in humans nor the respective disease state are considered.
35427693	0	0	theme	cancer	101:106	arg1	therapy					108:114	ultrasound-induced cancer therapy	82:114	ultrasound-induced cancer therapy	82:114	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	10	1	theme	Ultrasound	1606:1615	arg1	exposure					1617:1624	Ultrasound exposure	1606:1624	Ultrasound exposure for 10 min	1606:1635	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	5	2	theme	stabilized	886:895	arg1	nanodroplets					913:924	dextran stabilized perfluorohexane nanodroplets	878:924	dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug	878:958	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	1	3	theme	drug	248:251	arg1	systems					262:268	drug delivery systems	248:268	drug delivery systems	248:268	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	0	4	theme	ultrasound-induced	82:99	arg1	therapy					108:114	ultrasound-induced cancer therapy	82:114	ultrasound-induced cancer therapy	82:114	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	10	5	theme	triggered	1649:1657	arg1	release					1659:1665	triggered release	1649:1665	triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg)	1649:1799	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	5	6	theme	perfluorohexane	897:911	arg1	nanodroplets					913:924	dextran stabilized perfluorohexane nanodroplets	878:924	dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug	878:958	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	1	7	theme	delivery	253:260	arg1	systems					262:268	drug delivery systems	248:268	drug delivery systems	248:268	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	7	8	theme	homogenization	1133:1146	arg1	speed					1148:1152	the homogenization speed	1129:1152	the homogenization speed	1129:1152	In addition, by increasing the homogenization speed, the particle size was increased while entrapment efficiency was decreased.
35427693	2	9	dep	tackles	338:344	arg1	challenged					358:367	challenged	358:367	tackles with strict challenged which confine its broad application	338:403	Although, plenty of novel approaches are reported in this area, it tackles with strict challenged which confine its broad application.
35427693	9	10	theme	nanodroplets	1575:1586	arg1	composition					1593:1603	the nanodroplets (ND) composition	1571:1603	the nanodroplets (ND) composition	1571:1603	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	10	11	theme	sample	1712:1717	arg1	C3					1719:1720	sample C3	1712:1720	sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg)	1712:1799	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	10	12	theme	%	1726:1726	arg1	w/v					1727:1729	0.1%w/v dextran	1723:1737	0.1%w/v dextran	1723:1737	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	10	12	theme	%	1726:1726	arg1	homogenization					1740:1753	homogenization	1740:1753	homogenization speed of 24000 rpm and Dox content of 500 µg	1740:1798	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	9	13	from	incubation	1524:1533	arg1	PBS					1538:1540	PBS	1538:1540	PBS	1538:1540	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	8	14	theme	47.2 nm	1366:1372	arg1	values					1356:1361	optimum values	1348:1361	optimum values	1348:1361	On the other hand, by increasing the surfactant concentration, the particle size and entrapment efficiency reached to optimum values of 47.2 nm and 80.2%, respectively.
35427693	0	15	theme	novel	119:123	arg1	approach					143:150	A novel nanobiotechnology approach	117:150	A novel nanobiotechnology approach	117:150	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	8	16	theme	entrapment	1315:1324	arg1	efficiency					1326:1335	entrapment efficiency	1315:1335	entrapment efficiency	1315:1335	On the other hand, by increasing the surfactant concentration, the particle size and entrapment efficiency reached to optimum values of 47.2 nm and 80.2%, respectively.
35427693	8	17	theme	%	1382:1382	arg1	values					1356:1361	optimum values	1348:1361	optimum values	1348:1361	On the other hand, by increasing the surfactant concentration, the particle size and entrapment efficiency reached to optimum values of 47.2 nm and 80.2%, respectively.
35427693	5	18	theme	release	858:864	arg1	kinetics					866:873	drug release kinetics	853:873	drug release kinetics	853:873	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	4	19	theme	cancer	646:651	arg1	therapy					653:659	ultrasound-induced cancer therapy	627:659	ultrasound-induced cancer therapy	627:659	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	5	20	theme	parameters	710:719	arg1	effect					700:705	the effect	696:705	the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug	696:958	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	10	21	theme	Dox	1680:1682	arg1	Dox					1680:1682	Dox	1680:1682	Dox	1680:1682	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	10	21	theme	Dox	1680:1682	arg1	%					1675:1675	82.95%	1670:1675	82.95% of Dox	1670:1682	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	9	22	theme	doxorubicin	1427:1437	arg1	profile					1416:1422	In vitro release profile	1399:1422	In vitro release profile of doxorubicin	1399:1437	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	9	22	theme	doxorubicin	1427:1437	arg1	process					1474:1480	an apparently biphasic release process	1443:1480	an apparently biphasic release process	1443:1480	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	7	23	theme	particle	1159:1166	arg1	size					1168:1171	the particle size	1155:1171	the particle size	1155:1171	In addition, by increasing the homogenization speed, the particle size was increased while entrapment efficiency was decreased.
35427693	9	24	theme	release	1408:1414	arg1	profile					1416:1422	In vitro release profile	1399:1422	In vitro release profile of doxorubicin	1399:1437	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	9	24	theme	release	1408:1414	arg1	process					1474:1480	an apparently biphasic release process	1443:1480	an apparently biphasic release process	1443:1480	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	4	25	theme	ultrasound-induced	627:644	arg1	therapy					653:659	ultrasound-induced cancer therapy	627:659	ultrasound-induced cancer therapy	627:659	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	5	26	theme	surfactant	783:792	arg1	concentrations					794:807	surfactant concentrations	783:807	surfactant concentrations	783:807	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	10	27	dep	C3	1719:1720	arg1	w/v					1727:1729	0.1%w/v dextran	1723:1737	0.1%w/v dextran	1723:1737	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	10	27	dep	C3	1719:1720	arg1	homogenization					1740:1753	homogenization	1740:1753	homogenization speed of 24000 rpm and Dox content of 500 µg	1740:1798	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	10	28	from	formulation	1697:1707	arg1	release					1659:1665	triggered release	1649:1665	triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg)	1649:1799	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	9	29	dep	In	1399:1400	arg1	vitro					1402:1406	vitro	1402:1406	vitro	1402:1406	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	1	30	theme	biotechnology	190:202	arg1	approach					204:211	a biotechnology approach	188:211	a biotechnology approach	188:211	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	5	31	theme	concentrations	794:807	arg1	formulation					756:766	formulation	756:766	formulation	756:766	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	5	31	theme	concentrations	794:807	arg1	speed					746:750	homogenization speed	731:750	homogenization speed	731:750	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	8	32	theme	other	1237:1241	arg1	hand					1243:1246	the other hand	1233:1246	the other hand	1233:1246	On the other hand, by increasing the surfactant concentration, the particle size and entrapment efficiency reached to optimum values of 47.2 nm and 80.2%, respectively.
35427693	1	33	theme	approach	204:211	arg1	nanobiotechnology					157:173	The nanobiotechnology	153:173	The nanobiotechnology	153:173	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	1	33	theme	approach	204:211	arg1	heck					180:183	one heck	176:183	one heck of a biotechnology approach	176:211	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	10	34	theme	%	1675:1675	arg1	release					1659:1665	triggered release	1649:1665	triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg)	1649:1799	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	5	35	theme	work	678:681	arg1	aim					666:668	The aim	662:668	The aim of this work	662:681	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	9	36	theme	biphasic	1457:1464	arg1	process					1474:1480	an apparently biphasic release process	1443:1480	an apparently biphasic release process	1443:1480	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	9	36	theme	biphasic	1457:1464	arg1	profile					1416:1422	In vitro release profile	1399:1422	In vitro release profile of doxorubicin	1399:1437	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	2	37	theme	approaches	297:306	arg1	plenty					281:286	plenty	281:286	plenty of novel approaches	281:306	Although, plenty of novel approaches are reported in this area, it tackles with strict challenged which confine its broad application.
35427693	9	38	theme	In	1399:1400	arg1	profile					1416:1422	In vitro release profile	1399:1422	In vitro release profile of doxorubicin	1399:1437	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	9	38	theme	In	1399:1400	arg1	process					1474:1480	an apparently biphasic release process	1443:1480	an apparently biphasic release process	1443:1480	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	4	39	theme	stabilized	599:608	arg1	nanodroplets					610:621	phase shift dextran stabilized nanodroplets	579:621	phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy	579:659	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	5	40	theme	particle	812:819	arg1	size					821:824	particle size	812:824	particle size	812:824	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	6	41	theme	obtained	965:972	arg1	results					974:980	The obtained results	961:980	The obtained results	961:980	The obtained results showed that the particle size and encapsulation efficiency significantly increased by increasing polymer concentration.
35427693	0	42	theme	nanobiotechnology	125:141	arg1	approach					143:150	A novel nanobiotechnology approach	117:150	A novel nanobiotechnology approach	117:150	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	2	43	theme	novel	291:295	arg1	approaches					297:306	novel approaches	291:306	novel approaches	291:306	Although, plenty of novel approaches are reported in this area, it tackles with strict challenged which confine its broad application.
35427693	4	44	theme	dextran	591:597	arg1	nanodroplets					610:621	phase shift dextran stabilized nanodroplets	579:621	phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy	579:659	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	0	45	theme	shift	40:44	arg1	dextran					46:52	phase shift dextran	34:52	phase shift dextran	34:52	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	10	46	theme	Dox	1778:1780	arg1	content					1782:1788	Dox content	1778:1788	Dox content of 500 µg	1778:1798	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	8	47	theme	surfactant	1267:1276	arg1	concentration					1278:1290	the surfactant concentration	1263:1290	the surfactant concentration	1263:1290	On the other hand, by increasing the surfactant concentration, the particle size and entrapment efficiency reached to optimum values of 47.2 nm and 80.2%, respectively.
35427693	5	48	theme	entrapment	827:836	arg1	efficiency					838:847	entrapment efficiency	827:847	entrapment efficiency	827:847	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	0	49	theme	phase	34:38	arg1	dextran					46:52	phase shift dextran	34:52	phase shift dextran	34:52	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	6	50	theme	particle	998:1005	arg1	size					1007:1010	particle size	998:1010	particle size	998:1010	The obtained results showed that the particle size and encapsulation efficiency significantly increased by increasing polymer concentration.
35427693	6	51	theme	encapsulation	1016:1028	arg1	efficiency					1030:1039	encapsulation efficiency	1016:1039	encapsulation efficiency	1016:1039	The obtained results showed that the particle size and encapsulation efficiency significantly increased by increasing polymer concentration.
35427693	2	52	theme	broad	387:391	arg1	application					393:403	its broad application	383:403	its broad application	383:403	Although, plenty of novel approaches are reported in this area, it tackles with strict challenged which confine its broad application.
35427693	5	53	theme	dextran	771:777	arg1	formulation					756:766	formulation	756:766	formulation	756:766	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	5	53	theme	dextran	771:777	arg1	speed					746:750	homogenization speed	731:750	homogenization speed	731:750	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	9	54	theme	release	1466:1472	arg1	process					1474:1480	an apparently biphasic release process	1443:1480	an apparently biphasic release process	1443:1480	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	9	54	theme	release	1466:1472	arg1	profile					1416:1422	In vitro release profile	1399:1422	In vitro release profile of doxorubicin	1399:1437	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	1	55	from	point	239:243	arg1	systems					262:268	drug delivery systems	248:268	drug delivery systems	248:268	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	5	56	from	effect	700:705	arg1	size					821:824	particle size	812:824	particle size	812:824	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	5	56	from	effect	700:705	arg1	efficiency					838:847	entrapment efficiency	827:847	entrapment efficiency	827:847	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	5	56	from	effect	700:705	arg1	kinetics					866:873	drug release kinetics	853:873	drug release kinetics	853:873	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	10	57	theme	optimal	1689:1695	arg1	formulation					1697:1707	optimal formulation	1689:1707	optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg)	1689:1799	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	5	58	theme	nanodroplets	913:924	arg1	size					821:824	particle size	812:824	particle size	812:824	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	5	58	theme	nanodroplets	913:924	arg1	efficiency					838:847	entrapment efficiency	827:847	entrapment efficiency	827:847	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	5	58	theme	nanodroplets	913:924	arg1	kinetics					866:873	drug release kinetics	853:873	drug release kinetics	853:873	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	0	59	theme	dextran	46:52	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	0	59	theme	dextran	46:52	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy: A novel nanobiotechnology approach.
35427693	4	60	theme	nanobiotechnology	491:507	arg1	approach					509:516	a novel nanobiotechnology approach	483:516	a novel nanobiotechnology approach	483:516	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	5	61	contain	containing	926:935	arg1	nanodroplets					913:924	dextran stabilized perfluorohexane nanodroplets	878:924	dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug	878:958	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	5	61	contain	containing	926:935	arg2	drug					955:958	doxorubicin (Dox) drug	937:958	doxorubicin (Dox) drug	937:958	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	9	62	with	incubation	1524:1533	arg1	pH					1547:1548	pH	1547:1548	pH of 5.5	1547:1555	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	10	63	theme	dextran	1731:1737	arg1	w/v					1727:1729	0.1%w/v dextran	1723:1737	0.1%w/v dextran	1723:1737	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	10	63	theme	dextran	1731:1737	arg1	homogenization					1740:1753	homogenization	1740:1753	homogenization speed of 24000 rpm and Dox content of 500 µg	1740:1798	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	4	64	theme	novel	485:489	arg1	approach					509:516	a novel nanobiotechnology approach	483:516	a novel nanobiotechnology approach	483:516	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	5	65	theme	doxorubicin	937:947	arg1	drug					955:958	doxorubicin (Dox) drug	937:958	doxorubicin (Dox) drug	937:958	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	10	66	theme	C3	1719:1720	arg1	formulation					1697:1707	optimal formulation	1689:1707	optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg)	1689:1799	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	8	67	theme	particle	1297:1304	arg1	size					1306:1309	the particle size	1293:1309	the particle size	1293:1309	On the other hand, by increasing the surfactant concentration, the particle size and entrapment efficiency reached to optimum values of 47.2 nm and 80.2%, respectively.
35427693	10	68	theme	500 µg	1793:1798	arg1	24000 rpm					1764:1772	24000 rpm	1764:1772	24000 rpm	1764:1772	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	10	68	theme	500 µg	1793:1798	arg1	content					1782:1788	Dox content	1778:1788	Dox content of 500 µg	1778:1798	Ultrasound exposure for 10 min resulted in triggered release of 82.95% of Dox from optimal formulation of sample C3 (0.1%w/v dextran, homogenization speed of 24000 rpm and Dox content of 500 µg).
35427693	9	69	theme	24 h	1519:1522	arg1	incubation					1524:1533	24 h incubation	1519:1533	24 h incubation in PBS with pH of 5.5	1519:1555	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	1	70	theme	focal	233:237	arg1	point					239:243	a focal point	231:243	a focal point in drug delivery systems	231:268	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	1	70	theme	focal	233:237	arg1	nanobiotechnology					157:173	The nanobiotechnology	153:173	The nanobiotechnology	153:173	The nanobiotechnology, one heck of a biotechnology approach, is considered as a focal point in drug delivery systems.
35427693	9	71	theme	drug	1495:1498	arg1	%					1490:1490	7-13%	1486:1490	7-13% of drug	1486:1498	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	9	71	theme	drug	1495:1498	arg1	drug					1495:1498	drug	1495:1498	drug	1495:1498	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	4	72	theme	nanodroplets	610:621	arg1	synthesis					545:553	synthesis	545:553	synthesis	545:553	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	4	72	theme	nanodroplets	610:621	arg1	characterization					559:574	characterization	559:574	characterization	559:574	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	6	73	theme	polymer	1079:1085	arg1	concentration					1087:1099	polymer concentration	1079:1099	polymer concentration	1079:1099	The obtained results showed that the particle size and encapsulation efficiency significantly increased by increasing polymer concentration.
35427693	8	74	theme	optimum	1348:1354	arg1	values					1356:1361	optimum values	1348:1361	optimum values	1348:1361	On the other hand, by increasing the surfactant concentration, the particle size and entrapment efficiency reached to optimum values of 47.2 nm and 80.2%, respectively.
35427693	5	75	theme	drug	853:856	arg1	kinetics					866:873	drug release kinetics	853:873	drug release kinetics	853:873	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	4	76	theme	shift	585:589	arg1	nanodroplets					610:621	phase shift dextran stabilized nanodroplets	579:621	phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy	579:659	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
35427693	5	77	theme	homogenization	731:744	arg1	speed					746:750	homogenization speed	731:750	homogenization speed	731:750	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	7	78	theme	entrapment	1193:1202	arg1	efficiency					1204:1213	entrapment efficiency	1193:1213	entrapment efficiency	1193:1213	In addition, by increasing the homogenization speed, the particle size was increased while entrapment efficiency was decreased.
35427693	9	79	theme	5.5	1553:1555	arg1	pH					1547:1548	pH	1547:1548	pH of 5.5	1547:1555	In vitro release profile of doxorubicin was an apparently biphasic release process and 7-13% of drug was released after 24 h incubation in PBS with pH of 5.5, depending on the nanodroplets (ND) composition.
35427693	5	80	theme	dextran	878:884	arg1	nanodroplets					913:924	dextran stabilized perfluorohexane nanodroplets	878:924	dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug	878:958	The aim of this work was to study the effect of parameters including homogenization speed and formulation of dextran and surfactant concentrations on particle size, entrapment efficiency and drug release kinetics of dextran stabilized perfluorohexane nanodroplets containing doxorubicin (Dox) drug.
35427693	4	81	theme	phase	579:583	arg1	nanodroplets					610:621	phase shift dextran stabilized nanodroplets	579:621	phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy	579:659	Here, a novel nanobiotechnology approach is described in detail for synthesis and characterization of phase shift dextran stabilized nanodroplets for ultrasound-induced cancer therapy.
36055857	11	0	theme	dry	1616:1618	arg1	days					1594:1597	days	1594:1597	days in milk	1594:1605	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	0	theme	dry	1616:1618	arg1	intake					1627:1632	FA-free dry matter intake	1608:1632	FA-free dry matter intake	1608:1632	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	6	1	theme	sample	1003:1008	arg1	size					1010:1013	sample size	1003:1013	sample size	1003:1013	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	2	2	theme	lactating	304:312	arg1	cows					320:323	lactating dairy cows	304:323	lactating dairy cows	304:323	The objective of this meta-analysis was to develop models to predict milk fat concentration and yield of lactating dairy cows.
36055857	15	3	theme	dairy	2157:2161	arg1	cows					2163:2166	dairy cows	2157:2166	dairy cows	2157:2166	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	8	4	theme	mean	1264:1267	arg1	deviation					1280:1288	mean ± standard deviation	1264:1288	mean ± standard deviation	1264:1288	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	8	4	theme	mean	1264:1267	arg1	%					1261:1261	50.8 ± 10.3%	1250:1261	50.8 ± 10.3% (mean ± standard deviation)	1250:1289	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	5	5	theme	Lys	714:716	arg1	Met					719:721	Met	719:721	Met	719:721	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	Trp					734:736	Trp	734:736	Trp	734:736	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	Val					739:741	Val	739:741	Val	739:741	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	amounts					668:674	absorbed amounts	659:674	absorbed amounts (g/d)	659:680	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	Phe					724:726	Phe	724:726	Phe	724:726	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	g/d					677:679	g/d	677:679	g/d	677:679	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	Thr					729:731	Thr	729:731	Thr	729:731	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	FA					579:580	FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3)	579:653	FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3)	579:653	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	5	theme	Lys	714:716	arg1	Lys					714:716	Lys	714:716	Lys	714:716	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	7	6	theme	fat	1112:1114	arg1	yield					1116:1120	milk fat yield	1107:1120	milk fat yield (MFY)	1107:1126	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	7	6	theme	fat	1112:1114	arg1	MFY					1123:1125	MFY	1123:1125	MFY	1123:1125	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	6	7	theme	inference	821:829	arg1	method					831:836	A multi-model inference method	807:836	A multi-model inference method	807:836	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	12	8	from	intake	1770:1775	arg1	Ile					1809:1811	Ile	1809:1811	Ile	1809:1811	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	8	from	intake	1770:1775	arg1	milk					1786:1789	milk	1786:1789	milk	1786:1789	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	8	from	intake	1770:1775	arg1	Met					1801:1803	absorbed Met	1792:1803	absorbed Met	1792:1803	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	4	9	from	databases	430:438	arg1	Data					407:410	Data	407:410	Data from several feed databases	407:438	Data from several feed databases were used to calculate dietary nutrients when dietary nutrient composition was not reported.
36055857	5	10	dep	C18:3	648:652	arg1	C18:1					629:633	C18:1	629:633	C18:1	629:633	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	11	theme	acid	573:576	arg1	intake					542:547	Digested intake	533:547	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val)	533:742	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	11	theme	acid	573:576	arg1	variables					782:790	candidate variables	772:790	candidate variables in the models	772:804	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	11	theme	acid	573:576	arg1	DI					550:551	DI	550:551	DI	550:551	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	11	12	theme	best	1555:1558	arg1	days					1594:1597	days	1594:1597	days in milk	1594:1605	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	12	theme	best	1555:1558	arg1	variables					1560:1568	The best variables	1551:1568	The best variables for predicting MFC	1551:1587	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	5	13	from	variables	782:790	arg1	models					799:804	the models	795:804	the models	795:804	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	7	14	dep	4.78	1096:1099	arg1	to					1093:1094	to	1093:1094	to	1093:1094	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	13	15	theme	mean	1877:1880	arg1	error					1889:1893	a root mean square error	1870:1893	a root mean square error of 14.1%	1870:1902	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	12	16	theme	matter	1763:1768	arg1	intake					1770:1775	FA-free dry matter intake	1751:1775	FA-free dry matter intake	1751:1775	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	16	theme	matter	1763:1768	arg1	variables					1728:1736	The best predictor variables	1709:1736	The best predictor variables for MFY	1709:1744	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	7	17	theme	milk	1047:1050	arg1	fat					1052:1054	milk fat	1047:1054	Observed milk fat concentration (MFC)	1038:1074	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	0	18	theme	milk	18:21	arg1	fat					23:25	milk fat	18:25	milk fat concentration	18:39	Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis.
36055857	6	19	used	used	842:845	arg2	method					831:836	A multi-model inference method	807:836	A multi-model inference method	807:836	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	6	20	with	models	875:880	arg1	study					887:891	study	887:891	study	887:891	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	10	21	theme	other	1541:1545	arg1	FA					1547:1548	the other FA	1537:1548	the other FA	1537:1548	The DI of C18:2 (g/d) was more negatively correlated with MFC (-0.313) than that of the other FA.
36055857	14	22	theme	C18:3	1973:1977	arg1	DI					1967:1968	DI	1967:1968	DI of C18:3	1967:1977	Surprisingly, DI of C18:3 was positively related to milk fat, and this relationship was consistently observed among models.
36055857	12	23	theme	FA-free	1751:1757	arg1	intake					1770:1775	FA-free dry matter intake	1751:1775	FA-free dry matter intake	1751:1775	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	23	theme	FA-free	1751:1757	arg1	variables					1728:1736	The best predictor variables	1709:1736	The best predictor variables for MFY	1709:1744	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	24	theme	absorbed	1792:1799	arg1	Met					1801:1803	absorbed Met	1792:1803	absorbed Met	1792:1803	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	11	25	theme	C18:3	1670:1674	arg1	Trp					1704:1706	Trp	1704:1706	Trp	1704:1706	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	25	theme	C18:3	1670:1674	arg1	DI					1664:1665	DI	1664:1665	DI of C18:3	1664:1674	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	25	theme	C18:3	1670:1674	arg1	Met					1690:1692	absorbed Met	1681:1692	absorbed Met	1681:1692	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	25	theme	C18:3	1670:1674	arg1	starch					1643:1648	starch	1643:1648	starch	1643:1648	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	25	theme	C18:3	1670:1674	arg1	His					1695:1697	His	1695:1697	His	1695:1697	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	25	theme	C18:3	1670:1674	arg1	DI					1651:1652	DI	1651:1652	DI of C18:2	1651:1661	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	25	theme	C18:3	1670:1674	arg1	days					1594:1597	days	1594:1597	days in milk	1594:1605	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	25	theme	C18:3	1670:1674	arg1	intake					1627:1632	FA-free dry matter intake	1608:1632	FA-free dry matter intake	1608:1632	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	12	26	theme	best	1713:1716	arg1	intake					1770:1775	FA-free dry matter intake	1751:1775	FA-free dry matter intake	1751:1775	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	26	theme	best	1713:1716	arg1	variables					1728:1736	The best predictor variables	1709:1736	The best predictor variables for MFY	1709:1744	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	0	27	theme	dairy	64:68	arg1	cows					70:73	lactating dairy cows	54:73	lactating dairy cows	54:73	Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis.
36055857	1	28	theme	milk	136:139	arg1	fat					141:143	total milk fat	130:143	total milk fat	130:143	Few models have attempted to predict total milk fat because of its high variation among and within herds.
36055857	8	29	theme	Dietary	1175:1181	arg1	levels					1183:1188	Dietary levels	1175:1188	Dietary levels of forage, starch, and total FA (dry matter basis)	1175:1239	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	4	30	theme	several	417:423	arg1	databases					430:438	several feed databases	417:438	several feed databases	417:438	Data from several feed databases were used to calculate dietary nutrients when dietary nutrient composition was not reported.
36055857	5	31	dep	Lys	714:716	arg1	Leu					709:711	each AA (Arg, His, Ile, Leu, Lys	685:716	Leu	709:711	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	31	dep	Lys	714:716	arg1	Ile					704:706	each AA (Arg, His, Ile, Leu, Lys	685:716	Ile	704:706	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	31	dep	Lys	714:716	arg1	His					699:701	each AA (Arg, His, Ile, Leu, Lys	685:716	His	699:701	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	15	32	theme	fat	2237:2239	arg1	yield					2241:2245	fat yield	2237:2245	fat yield	2237:2245	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	12	33	theme	C18:3	1848:1852	arg1	intake					1770:1775	FA-free dry matter intake	1751:1775	FA-free dry matter intake	1751:1775	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	33	theme	C18:3	1848:1852	arg1	variables					1728:1736	The best predictor variables	1709:1736	The best predictor variables for MFY	1709:1744	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	33	theme	C18:3	1848:1852	arg1	intakes					1818:1824	intakes	1818:1824	intakes of digested C16:0 and C18:3	1818:1852	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	33	theme	C18:3	1848:1852	arg1	days					1778:1781	days	1778:1781	days in milk, absorbed Met and Ile	1778:1811	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	15	34	theme	practical	2115:2123	arg1	tool					2125:2128	a practical tool	2113:2128	a practical tool for predicting milk fat of dairy cows	2113:2166	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	15	34	theme	practical	2115:2123	arg1	models					2081:2086	The models	2077:2086	The models developed	2077:2096	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	6	35	theme	multi-model	809:819	arg1	method					831:836	A multi-model inference method	807:836	A multi-model inference method	807:836	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	12	36	theme	C16:0	1838:1842	arg1	intake					1770:1775	FA-free dry matter intake	1751:1775	FA-free dry matter intake	1751:1775	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	36	theme	C16:0	1838:1842	arg1	variables					1728:1736	The best predictor variables	1709:1736	The best predictor variables for MFY	1709:1744	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	36	theme	C16:0	1838:1842	arg1	intakes					1818:1824	intakes	1818:1824	intakes of digested C16:0 and C18:3	1818:1852	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	36	theme	C16:0	1838:1842	arg1	days					1778:1781	days	1778:1781	days in milk, absorbed Met and Ile	1778:1811	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	6	37	theme	random	900:905	arg1	effect					907:912	the random effect	896:912	the random effect	896:912	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	15	38	theme	cows	2163:2166	arg1	fat					2150:2152	milk fat	2145:2152	milk fat of dairy cows	2145:2166	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	3	39	theme	658	362:364	arg1	treatments					366:375	658 treatments	362:375	658 treatments	362:375	Data from 158 studies consisting of 658 treatments from 2,843 animals were used.
36055857	8	40	theme	total	1213:1217	arg1	FA					1219:1220	total FA	1213:1220	total FA (dry matter basis)	1213:1239	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	8	40	theme	total	1213:1217	arg1	basis					1234:1238	dry matter basis	1223:1238	dry matter basis	1223:1238	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	0	41	theme	fat	23:25	arg1	concentration					27:39	milk fat concentration	18:39	milk fat concentration	18:39	Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis.
36055857	5	42	theme	candidate	772:780	arg1	intake					542:547	Digested intake	533:547	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val)	533:742	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	42	theme	candidate	772:780	arg1	variables					782:790	candidate variables	772:790	candidate variables in the models	772:804	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	11	43	theme	C18:2	1657:1661	arg1	Trp					1704:1706	Trp	1704:1706	Trp	1704:1706	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	43	theme	C18:2	1657:1661	arg1	DI					1664:1665	DI	1664:1665	DI of C18:3	1664:1674	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	43	theme	C18:2	1657:1661	arg1	Met					1690:1692	absorbed Met	1681:1692	absorbed Met	1681:1692	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	43	theme	C18:2	1657:1661	arg1	starch					1643:1648	starch	1643:1648	starch	1643:1648	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	43	theme	C18:2	1657:1661	arg1	His					1695:1697	His	1695:1697	His	1695:1697	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	43	theme	C18:2	1657:1661	arg1	DI					1651:1652	DI	1651:1652	DI of C18:2	1651:1661	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	43	theme	C18:2	1657:1661	arg1	days					1594:1597	days	1594:1597	days in milk	1594:1605	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	43	theme	C18:2	1657:1661	arg1	intake					1627:1632	FA-free dry matter intake	1608:1632	FA-free dry matter intake	1608:1632	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	5	44	used	used	764:767	arg2	variables					782:790	candidate variables	772:790	candidate variables in the models	772:804	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	44	used	used	764:767	arg2	DI					550:551	DI	550:551	DI	550:551	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	44	used	used	764:767	arg2	intake					542:547	Digested intake	533:547	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val)	533:742	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	45	theme	absorbed	659:666	arg1	g/d					677:679	g/d	677:679	g/d	677:679	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	45	theme	absorbed	659:666	arg1	Lys					714:716	Lys	714:716	Lys	714:716	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	45	theme	absorbed	659:666	arg1	amounts					668:674	absorbed amounts	659:674	absorbed amounts (g/d)	659:680	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	6	46	theme	information	967:977	arg1	criterion					979:987	Akaike's information criterion	958:987	Akaike's information criterion corrected for sample size	958:1013	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	15	47	used	used	2105:2108	arg2	models					2081:2086	The models	2077:2086	The models developed	2077:2096	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	15	47	used	used	2105:2108	arg2	tool					2125:2128	a practical tool	2113:2128	a practical tool for predicting milk fat of dairy cows	2113:2166	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	8	48	theme	matter	1227:1232	arg1	FA					1219:1220	total FA	1213:1220	total FA (dry matter basis)	1213:1239	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	8	48	theme	matter	1227:1232	arg1	basis					1234:1238	dry matter basis	1223:1238	dry matter basis	1223:1238	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	8	49	theme	±	1269:1269	arg1	deviation					1280:1288	mean ± standard deviation	1264:1288	mean ± standard deviation	1264:1288	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	8	49	theme	±	1269:1269	arg1	%					1261:1261	50.8 ± 10.3%	1250:1261	50.8 ± 10.3% (mean ± standard deviation)	1250:1289	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	0	50	dep	predict	10:16	arg1	meta-analysis					78:90	A meta-analysis	76:90	A meta-analysis	76:90	Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis.
36055857	6	51	theme	large	856:860	arg1	set					862:864	a large set	854:864	a large set of mixed models with study as the random effect	854:912	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	6	51	theme	large	856:860	arg1	models					875:880	mixed models	869:880	mixed models with study as the random effect	869:912	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	5	52	dep	DI	550:551	arg1	g/d					554:556	g/d	554:556	g/d	554:556	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	8	53	theme	50.8	1250:1253	arg1	±					1255:1255	±	1255:1255	±	1255:1255	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	10	54	theme	C18:2	1463:1467	arg1	DI					1457:1458	The DI	1453:1458	The DI of C18:2 (g/d)	1453:1473	The DI of C18:2 (g/d) was more negatively correlated with MFC (-0.313) than that of the other FA.
36055857	13	55	theme	concordance	1908:1918	arg1	coefficient					1932:1942	concordance correlation coefficient	1908:1942	concordance correlation coefficient of 0.81	1908:1950	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	13	56	theme	correlation	1920:1930	arg1	coefficient					1932:1942	concordance correlation coefficient	1908:1942	concordance correlation coefficient of 0.81	1908:1950	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	2	57	theme	dairy	314:318	arg1	cows					320:323	lactating dairy cows	304:323	lactating dairy cows	304:323	The objective of this meta-analysis was to develop models to predict milk fat concentration and yield of lactating dairy cows.
36055857	11	58	theme	matter	1620:1625	arg1	days					1594:1597	days	1594:1597	days in milk	1594:1605	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	58	theme	matter	1620:1625	arg1	intake					1627:1632	FA-free dry matter intake	1608:1632	FA-free dry matter intake	1608:1632	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	8	59	theme	10.3	1257:1260	arg1	±					1255:1255	±	1255:1255	±	1255:1255	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	9	60	dep	dietary	1374:1380	arg1	forage					1382:1387	forage	1382:1387	forage	1382:1387	The MFC was positively correlated with dietary forage (0.294) and negatively associated with dietary starch (-0.286).
36055857	8	61	theme	±	1297:1297	arg1	%					1302:1302	27.5 ± 7.0%	1292:1302	27.5 ± 7.0%	1292:1302	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	11	62	theme	FA-free	1608:1614	arg1	days					1594:1597	days	1594:1597	days in milk	1594:1605	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	11	62	theme	FA-free	1608:1614	arg1	intake					1627:1632	FA-free dry matter intake	1608:1632	FA-free dry matter intake	1608:1632	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	5	63	theme	fatty	567:571	arg1	acid					573:576	each fatty acid	562:576	each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val)	562:742	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	15	64	theme	milk	2145:2148	arg1	fat					2150:2152	milk fat	2145:2152	milk fat of dairy cows	2145:2166	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	2	65	theme	fat	273:275	arg1	concentration					277:289	milk fat concentration	268:289	milk fat concentration	268:289	The objective of this meta-analysis was to develop models to predict milk fat concentration and yield of lactating dairy cows.
36055857	7	66	theme	milk	1107:1110	arg1	yield					1116:1120	milk fat yield	1107:1120	milk fat yield (MFY)	1107:1126	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	7	66	theme	milk	1107:1110	arg1	MFY					1123:1125	MFY	1123:1125	MFY	1123:1125	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	13	67	theme	root	1872:1875	arg1	error					1889:1893	a root mean square error	1870:1893	a root mean square error of 14.1%	1870:1902	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	8	68	theme	±	1313:1313	arg1	%					1318:1318	3.4 ± 1.3%	1309:1318	3.4 ± 1.3%	1309:1318	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	1	69	theme	high	160:163	arg1	variation					165:173	its high variation	156:173	its high variation among and within herds	156:196	Few models have attempted to predict total milk fat because of its high variation among and within herds.
36055857	2	70	theme	milk	268:271	arg1	fat					273:275	milk fat	268:275	milk fat concentration	268:289	The objective of this meta-analysis was to develop models to predict milk fat concentration and yield of lactating dairy cows.
36055857	6	71	theme	models	875:880	arg1	set					862:864	a large set	854:864	a large set of mixed models with study as the random effect	854:912	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	6	71	theme	models	875:880	arg1	models					875:880	mixed models	869:880	mixed models with study as the random effect	869:912	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	13	72	theme	square	1882:1887	arg1	error					1889:1893	a root mean square error	1870:1893	a root mean square error of 14.1%	1870:1902	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	14	73	theme	milk	2005:2008	arg1	fat					2010:2012	milk fat	2005:2012	milk fat	2005:2012	Surprisingly, DI of C18:3 was positively related to milk fat, and this relationship was consistently observed among models.
36055857	4	74	theme	dietary	486:492	arg1	composition					503:513	dietary nutrient composition	486:513	dietary nutrient composition	486:513	Data from several feed databases were used to calculate dietary nutrients when dietary nutrient composition was not reported.
36055857	1	75	theme	Few	93:95	arg1	models					97:102	Few models	93:102	Few models	93:102	Few models have attempted to predict total milk fat because of its high variation among and within herds.
36055857	12	76	from	intakes	1818:1824	arg1	Ile					1809:1811	Ile	1809:1811	Ile	1809:1811	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	76	from	intakes	1818:1824	arg1	milk					1786:1789	milk	1786:1789	milk	1786:1789	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	76	from	intakes	1818:1824	arg1	Met					1801:1803	absorbed Met	1792:1803	absorbed Met	1792:1803	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	2	77	theme	meta-analysis	221:233	arg1	objective					203:211	The objective	199:211	The objective of this meta-analysis	199:233	The objective of this meta-analysis was to develop models to predict milk fat concentration and yield of lactating dairy cows.
36055857	12	78	theme	dry	1759:1761	arg1	intake					1770:1775	FA-free dry matter intake	1751:1775	FA-free dry matter intake	1751:1775	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	78	theme	dry	1759:1761	arg1	variables					1728:1736	The best predictor variables	1709:1736	The best predictor variables for MFY	1709:1744	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	6	79	theme	best	923:926	arg1	models					928:933	the best models	919:933	the best models	919:933	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	11	80	from	days	1594:1597	arg1	milk					1602:1605	milk	1602:1605	milk	1602:1605	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	0	81	theme	lactating	54:62	arg1	cows					70:73	lactating dairy cows	54:73	lactating dairy cows	54:73	Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis.
36055857	11	82	theme	absorbed	1681:1688	arg1	Met					1690:1692	absorbed Met	1681:1692	absorbed Met	1681:1692	The best variables for predicting MFC were days in milk, FA-free dry matter intake, forage, starch, DI of C18:2, DI of C18:3, and absorbed Met, His, and Trp.
36055857	12	83	theme	predictor	1718:1726	arg1	intake					1770:1775	FA-free dry matter intake	1751:1775	FA-free dry matter intake	1751:1775	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	83	theme	predictor	1718:1726	arg1	variables					1728:1736	The best predictor variables	1709:1736	The best predictor variables for MFY	1709:1744	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	0	84	theme	cows	70:73	arg1	concentration					27:39	milk fat concentration	18:39	milk fat concentration	18:39	Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis.
36055857	0	84	theme	cows	70:73	arg1	yield					45:49	yield	45:49	yield	45:49	Models to predict milk fat concentration and yield of lactating dairy cows: A meta-analysis.
36055857	1	85	theme	total	130:134	arg1	fat					141:143	total milk fat	130:143	total milk fat	130:143	Few models have attempted to predict total milk fat because of its high variation among and within herds.
36055857	4	86	theme	feed	425:428	arg1	databases					430:438	several feed databases	417:438	several feed databases	417:438	Data from several feed databases were used to calculate dietary nutrients when dietary nutrient composition was not reported.
36055857	4	87	theme	dietary	463:469	arg1	nutrients					471:479	dietary nutrients	463:479	dietary nutrients	463:479	Data from several feed databases were used to calculate dietary nutrients when dietary nutrient composition was not reported.
36055857	8	88	theme	forage	1193:1198	arg1	levels					1183:1188	Dietary levels	1175:1188	Dietary levels of forage, starch, and total FA (dry matter basis)	1175:1239	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	7	89	theme	fat	1052:1054	arg1	MFC					1071:1073	MFC	1071:1073	MFC	1071:1073	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	7	89	theme	fat	1052:1054	arg1	concentration					1056:1068	Observed milk fat concentration	1038:1068	Observed milk fat concentration (MFC)	1038:1074	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	8	90	theme	starch	1201:1206	arg1	levels					1183:1188	Dietary levels	1175:1188	Dietary levels of forage, starch, and total FA (dry matter basis)	1175:1239	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	12	91	from	days	1778:1781	arg1	Ile					1809:1811	Ile	1809:1811	Ile	1809:1811	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	91	from	days	1778:1781	arg1	milk					1786:1789	milk	1786:1789	milk	1786:1789	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	12	91	from	days	1778:1781	arg1	Met					1801:1803	absorbed Met	1792:1803	absorbed Met	1792:1803	The best predictor variables for MFY were FA-free dry matter intake, days in milk, absorbed Met and Ile, and intakes of digested C16:0 and C18:3.
36055857	3	92	from	studies	340:346	arg1	Data					326:329	Data	326:329	Data from 158 studies consisting of 658 treatments from 2,843 animals	326:394	Data from 158 studies consisting of 658 treatments from 2,843 animals were used.
36055857	5	93	theme	Digested	533:540	arg1	intake					542:547	Digested intake	533:547	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val)	533:742	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	93	theme	Digested	533:540	arg1	variables					782:790	candidate variables	772:790	candidate variables in the models	772:804	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	93	theme	Digested	533:540	arg1	DI					550:551	DI	550:551	DI	550:551	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	7	94	theme	Observed	1038:1045	arg1	MFC					1071:1073	MFC	1071:1073	MFC	1071:1073	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	7	94	theme	Observed	1038:1045	arg1	concentration					1056:1068	Observed milk fat concentration	1038:1068	Observed milk fat concentration (MFC)	1038:1074	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	15	95	theme	additional	2192:2201	arg1	factors					2203:2209	additional factors	2192:2209	additional factors	2192:2209	The models developed can be used as a practical tool for predicting milk fat of dairy cows, while recognizing that additional factors are likely to also affect fat yield.
36055857	3	96	used	used	401:404	arg2	Data					326:329	Data	326:329	Data from 158 studies consisting of 658 treatments from 2,843 animals	326:394	Data from 158 studies consisting of 658 treatments from 2,843 animals were used.
36055857	4	97	used	used	445:448	arg2	Data					407:410	Data	407:410	Data from several feed databases	407:438	Data from several feed databases were used to calculate dietary nutrients when dietary nutrient composition was not reported.
36055857	7	98	dep	1.787	1149:1153	arg1	to					1146:1147	to	1146:1147	to	1146:1147	Observed milk fat concentration (MFC) ranged from 2.26 to 4.78%, and milk fat yield (MFY) ranged from 0.488 to 1.787 kg/d among studies.
36055857	5	99	dep	FA	579:580	arg1	C18:3					648:652	C18:3	648:652	C18:3	648:652	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	8	100	theme	FA	1219:1220	arg1	levels					1183:1188	Dietary levels	1175:1188	Dietary levels of forage, starch, and total FA (dry matter basis)	1175:1239	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	13	101	contain	had	1866:1868	arg2	error					1889:1893	a root mean square error	1870:1893	a root mean square error of 14.1%	1870:1902	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	13	101	contain	had	1866:1868	arg2	coefficient					1932:1942	concordance correlation coefficient	1908:1942	concordance correlation coefficient of 0.81	1908:1950	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	13	101	contain	had	1866:1868	arg1	model					1860:1864	This model	1855:1864	This model	1855:1864	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	13	102	theme	0.81	1947:1950	arg1	coefficient					1932:1942	concordance correlation coefficient	1908:1942	concordance correlation coefficient of 0.81	1908:1950	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	13	102	theme	0.81	1947:1950	arg1	error					1889:1893	a root mean square error	1870:1893	a root mean square error of 14.1%	1870:1902	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	5	103	dep	acid	573:576	arg1	Met					719:721	Met	719:721	Met	719:721	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	Trp					734:736	Trp	734:736	Trp	734:736	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	Val					739:741	Val	739:741	Val	739:741	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	amounts					668:674	absorbed amounts	659:674	absorbed amounts (g/d)	659:680	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	Phe					724:726	Phe	724:726	Phe	724:726	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	g/d					677:679	g/d	677:679	g/d	677:679	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	Thr					729:731	Thr	729:731	Thr	729:731	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	FA					579:580	FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3)	579:653	FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3)	579:653	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	5	103	dep	acid	573:576	arg1	Lys					714:716	Lys	714:716	Lys	714:716	Digested intake (DI, g/d) of each fatty acid (FA; C12:0, C14:0, C16:0, C16:1, C18:0, C18:1 cis, C18:1 trans C18:2, C18:3) and absorbed amounts (g/d) of each AA (Arg, His, Ile, Leu, Lys, Met, Phe, Thr, Trp, Val) were calculated and used as candidate variables in the models.
36055857	8	104	theme	dry	1223:1225	arg1	FA					1219:1220	total FA	1213:1220	total FA (dry matter basis)	1213:1239	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	8	104	theme	dry	1223:1225	arg1	basis					1234:1238	dry matter basis	1223:1238	dry matter basis	1223:1238	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	13	105	theme	%	1902:1902	arg1	coefficient					1932:1942	concordance correlation coefficient	1908:1942	concordance correlation coefficient of 0.81	1908:1950	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	13	105	theme	%	1902:1902	arg1	error					1889:1893	a root mean square error	1870:1893	a root mean square error of 14.1%	1870:1902	This model had a root mean square error of 14.1% and concordance correlation coefficient of 0.81.
36055857	6	106	theme	mixed	869:873	arg1	models					875:880	mixed models	869:880	mixed models with study as the random effect	869:912	A multi-model inference method was used to fit a large set of mixed models with study as the random effect, and the best models were selected based on Akaike's information criterion corrected for sample size and evaluated further.
36055857	4	107	theme	nutrient	494:501	arg1	composition					503:513	dietary nutrient composition	486:513	dietary nutrient composition	486:513	Data from several feed databases were used to calculate dietary nutrients when dietary nutrient composition was not reported.
36055857	8	108	theme	standard	1271:1278	arg1	deviation					1280:1288	mean ± standard deviation	1264:1288	mean ± standard deviation	1264:1288	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	8	108	theme	standard	1271:1278	arg1	%					1261:1261	50.8 ± 10.3%	1250:1261	50.8 ± 10.3% (mean ± standard deviation)	1250:1289	Dietary levels of forage, starch, and total FA (dry matter basis) averaged 50.8 ± 10.3% (mean ± standard deviation), 27.5 ± 7.0%, and 3.4 ± 1.3%, respectively.
36055857	9	109	theme	dietary	1428:1434	arg1	starch					1436:1441	dietary starch	1428:1441	dietary starch (-0.286)	1428:1450	The MFC was positively correlated with dietary forage (0.294) and negatively associated with dietary starch (-0.286).
36055857	9	109	theme	dietary	1428:1434	arg1	-0.286					1444:1449	-0.286	1444:1449	-0.286	1444:1449	The MFC was positively correlated with dietary forage (0.294) and negatively associated with dietary starch (-0.286).
36055857	2	110	theme	cows	320:323	arg1	yield					295:299	yield	295:299	yield	295:299	The objective of this meta-analysis was to develop models to predict milk fat concentration and yield of lactating dairy cows.
36055857	2	110	theme	cows	320:323	arg1	concentration					277:289	milk fat concentration	268:289	milk fat concentration	268:289	The objective of this meta-analysis was to develop models to predict milk fat concentration and yield of lactating dairy cows.
36014816	6	0	theme	supplementation	1144:1158	arg1	weeks					1130:1134	four weeks	1125:1134	four weeks of Tana supplementation	1125:1158	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	2	1	theme	most	350:353	arg1	ways					362:365	the most direct ways	346:365	the most direct ways	346:365	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	9	2	theme	exercise	1941:1948	arg1	performance					1950:1960	exercise performance	1941:1960	exercise performance	1941:1960	Taken together, Tana supplementation for four weeks was effective in improving the gut microbiota, thereby enhancing exercise performance, and had antifatigue effects.
36014816	8	3	theme	increasing	1696:1705	arg1	doses					1707:1711	increasing doses	1696:1711	increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut	1696:1821	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	5	4	theme	Cancer	893:898	arg1	mice					915:918	40 male Institute of Cancer Research (ICR) mice	872:918	40 male Institute of Cancer Research (ICR) mice	872:918	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	7	5	theme	urea	1481:1484	arg1	BUN					1496:1498	BUN	1496:1498	BUN	1496:1498	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	7	5	theme	urea	1481:1484	arg1	nitrogen					1486:1493	blood urea nitrogen	1475:1493	blood urea nitrogen (BUN)	1475:1499	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	8	6	theme	supplementation	1721:1735	arg1	doses					1707:1711	increasing doses	1696:1711	increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut	1696:1821	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	5	7	theme	×	1015:1015	arg1	CFU/mice/day					1021:1032	6.15 × 107 CFU/mice/day	1010:1032	6.15 × 107 CFU/mice/day	1010:1032	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	5	7	theme	×	1015:1015	arg1	Tana-1×					1001:1007	(2) Tana-1×	997:1007	(2) Tana-1× (6.15 × 107 CFU/mice/day)	997:1033	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	7	8	with	supplementation	1363:1377	arg1	Tana					1384:1387	Tana	1384:1387	Tana	1384:1387	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	8	9	from	abundance	1769:1777	arg1	gut					1819:1821	the gut	1815:1821	the gut	1815:1821	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	5	10	dep	vehicle	971:977	arg1	1					968:968	1	968:968	1	968:968	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	6	11	theme	grip	1179:1182	arg1	strength					1184:1191	the grip strength	1175:1191	the grip strength	1175:1191	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	5	12	theme	Institute	880:888	arg1	mice					915:918	40 male Institute of Cancer Research (ICR) mice	872:918	40 male Institute of Cancer Research (ICR) mice	872:918	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	7	13	theme	ammonia	1462:1468	arg1	levels					1501:1506	lactate, ammonia, and blood urea nitrogen (BUN) levels	1453:1506	lactate, ammonia, and blood urea nitrogen (BUN) levels	1453:1506	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	9	14	contain	had	1967:1969	arg1	supplementation					1845:1859	Tana supplementation	1840:1859	Tana supplementation for four weeks	1840:1874	Taken together, Tana supplementation for four weeks was effective in improving the gut microbiota, thereby enhancing exercise performance, and had antifatigue effects.
36014816	9	14	contain	had	1967:1969	arg2	effects					1983:1989	antifatigue effects	1971:1989	antifatigue effects	1971:1989	Taken together, Tana supplementation for four weeks was effective in improving the gut microbiota, thereby enhancing exercise performance, and had antifatigue effects.
36014816	7	15	theme	lactate	1453:1459	arg1	levels					1501:1506	lactate, ammonia, and blood urea nitrogen (BUN) levels	1453:1506	lactate, ammonia, and blood urea nitrogen (BUN) levels	1453:1506	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	5	16	dep	Tana-5×	1084:1090	arg1	4					1081:1081	4	1081:1081	4	1081:1081	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	4	17	theme	Olympic	716:722	arg1	Games					724:728	the Olympic Games	712:728	the Olympic Games	712:728	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	4	17	theme	Olympic	716:722	arg1	World					688:692	the World	684:692	the World	684:692	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	6	18	from	storage	1239:1245	arg1	muscle					1264:1269	muscle	1264:1269	muscle	1264:1269	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	6	18	from	storage	1239:1245	arg1	liver					1254:1258	liver	1254:1258	liver	1254:1258	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	7	19	theme	fatigue-related	1416:1430	arg1	markers					1444:1450	fatigue-related biochemical markers	1416:1450	fatigue-related biochemical markers	1416:1450	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	5	20	dep	Tana-2×	1040:1046	arg1	3					1037:1037	3	1037:1037	3	1037:1037	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	0	21	theme	Exercise	98:105	arg1	Performance					107:117	Exercise Performance	98:117	Exercise Performance	98:117	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	4	22	theme	exercise	797:804	arg1	performance					806:816	exercise performance	797:816	exercise performance	797:816	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	6	23	from	performance	1213:1223	arg1	muscle					1264:1269	muscle	1264:1269	muscle	1264:1269	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	6	23	from	performance	1213:1223	arg1	liver					1254:1258	liver	1254:1258	liver	1254:1258	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	8	24	theme	exercise	1613:1620	arg1	dose-dependent					1678:1691	dose-dependent	1678:1691	dose-dependent	1678:1691	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	8	24	theme	exercise	1613:1620	arg1	performance					1622:1632	the improved exercise performance	1600:1632	the improved exercise performance	1600:1632	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	7	25	theme	creatine	1512:1519	arg1	CK					1529:1530	CK	1529:1530	CK	1529:1530	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	7	25	theme	creatine	1512:1519	arg1	kinase					1521:1526	creatine kinase	1512:1526	creatine kinase (CK) activity	1512:1540	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	0	26	theme	Antifatigue	132:142	arg1	Effects					144:150	Antifatigue Effects	132:150	Antifatigue Effects in Mice	132:158	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	4	27	theme	Tana	762:765	arg1	supplementation					767:781	Tana supplementation	762:781	Tana supplementation	762:781	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	0	28	theme	Probiotic	0:8	arg1	Tana					40:43	Probiotic Lactiplantibacillus plantarum Tana	0:43	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter	0:87	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	6	29	from	strength	1184:1191	arg1	muscle					1264:1269	muscle	1264:1269	muscle	1264:1269	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	6	29	from	strength	1184:1191	arg1	liver					1254:1258	liver	1254:1258	liver	1254:1258	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	8	30	from	ratio	1783:1787	arg1	gut					1819:1821	the gut	1815:1821	the gut	1815:1821	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	9	31	theme	Tana	1840:1843	arg1	supplementation					1845:1859	Tana supplementation	1840:1859	Tana supplementation for four weeks	1840:1874	Taken together, Tana supplementation for four weeks was effective in improving the gut microbiota, thereby enhancing exercise performance, and had antifatigue effects.
36014816	3	32	theme	human-origin	449:460	arg1	probiotics					462:471	human-origin probiotics	449:471	human-origin probiotics	449:471	In recent years, the development and application of human-origin probiotics has gradually attracted attention.
36014816	0	33	theme	plantarum	30:38	arg1	Tana					40:43	Probiotic Lactiplantibacillus plantarum Tana	0:43	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter	0:87	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	6	34	dep	liver	1254:1258	arg1	the					1250:1252	the	1250:1252	the	1250:1252	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	7	35	theme	nitrogen	1486:1493	arg1	levels					1501:1506	lactate, ammonia, and blood urea nitrogen (BUN) levels	1453:1506	lactate, ammonia, and blood urea nitrogen (BUN) levels	1453:1506	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	3	36	theme	recent	400:405	arg1	years					407:411	recent years	400:411	recent years	400:411	In recent years, the development and application of human-origin probiotics has gradually attracted attention.
36014816	5	37	theme	of	890:891	arg1	mice					915:918	40 male Institute of Cancer Research (ICR) mice	872:918	40 male Institute of Cancer Research (ICR) mice	872:918	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	8	38	theme	beneficial	1792:1801	arg1	bacteria					1803:1810	beneficial bacteria	1792:1810	beneficial bacteria	1792:1810	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	4	39	theme	Lactiplantibacillus	542:560	arg1	“Tana”					572:577	intestinal Lactiplantibacillus plantarum “Tana”	531:577	intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice	531:858	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	4	40	theme	antifatigue	832:842	arg1	effects					844:850	antifatigue effects	832:850	antifatigue effects in mice	832:858	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	6	41	theme	p	1339:1339	arg1	group					1332:1336	the vehicle group	1320:1336	the vehicle group (p < 0.05)	1320:1347	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	6	41	theme	p	1339:1339	arg1	<					1341:1341	p < 0.05	1339:1346	p < 0.05	1339:1346	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	5	42	theme	×	1098:1098	arg1	CFU/mice/day					1104:1115	3.09 × 108 CFU/mice/day	1093:1115	3.09 × 108 CFU/mice/day	1093:1115	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	5	42	theme	×	1098:1098	arg1	Tana-5×					1084:1090	(4) Tana-5×	1080:1090	(4) Tana-5× (3.09 × 108 CFU/mice/day)	1080:1116	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	8	43	theme	antifatigue	1638:1648	arg1	benefits					1650:1657	antifatigue benefits	1638:1657	antifatigue benefits	1638:1657	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	9	44	theme	gut	1907:1909	arg1	microbiota					1911:1920	the gut microbiota	1903:1920	the gut microbiota	1903:1920	Taken together, Tana supplementation for four weeks was effective in improving the gut microbiota, thereby enhancing exercise performance, and had antifatigue effects.
36014816	2	45	theme	direct	355:360	arg1	ways					362:365	the most direct ways	346:365	the most direct ways	346:365	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	1	46	theme	microbiota	248:257	arg1	composition					225:235	the composition	221:235	the composition of the gut microbiota	221:257	Exercise causes changes in the gut microbiota, and in turn, the composition of the gut microbiota affects exercise performance.
36014816	2	47	from	one	339:341	arg1	addition					292:299	addition	292:299	addition	292:299	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	6	48	theme	Tana	1139:1142	arg1	supplementation					1144:1158	Tana supplementation	1139:1158	Tana supplementation	1139:1158	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	1	49	theme	exercise	267:274	arg1	performance					276:286	exercise performance	267:286	exercise performance	267:286	Exercise causes changes in the gut microbiota, and in turn, the composition of the gut microbiota affects exercise performance.
36014816	5	50	dep	Tana-1×	1001:1007	arg1	2					998:998	2	998:998	2	998:998	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	1	51	theme	gut	192:194	arg1	microbiota					196:205	the gut microbiota	188:205	the gut microbiota	188:205	Exercise causes changes in the gut microbiota, and in turn, the composition of the gut microbiota affects exercise performance.
36014816	8	52	dep	abundance	1769:1777	arg1	the					1765:1767	the	1765:1767	the	1765:1767	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	0	53	attach	Isolated	45:52	arg1	Weightlifter					76:87	Weightlifter	76:87	Weightlifter	76:87	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	0	53	attach	Isolated	45:52	arg2	Tana					40:43	Probiotic Lactiplantibacillus plantarum Tana	0:43	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter	0:87	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	6	54	theme	glycogen	1230:1237	arg1	storage					1239:1245	glycogen storage	1230:1245	glycogen storage	1230:1245	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	8	55	theme	Tana	1716:1719	arg1	supplementation					1721:1735	Tana supplementation	1716:1735	Tana supplementation (p < 0.05)	1716:1746	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	8	55	theme	Tana	1716:1719	arg1	<					1740:1740	p < 0.05	1738:1745	p < 0.05	1738:1745	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	2	56	theme	probiotics	325:334	arg1	supplementation					306:320	the supplementation	302:320	the supplementation of probiotics	302:334	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	2	56	theme	probiotics	325:334	arg1	ways					362:365	the most direct ways	346:365	the most direct ways	346:365	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	2	56	theme	probiotics	325:334	arg1	one					339:341	one	339:341	one	339:341	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	5	57	theme	Research	900:907	arg1	mice					915:918	40 male Institute of Cancer Research (ICR) mice	872:918	40 male Institute of Cancer Research (ICR) mice	872:918	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	7	58	theme	blood	1475:1479	arg1	BUN					1496:1498	BUN	1496:1498	BUN	1496:1498	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	7	58	theme	blood	1475:1479	arg1	nitrogen					1486:1493	blood urea nitrogen	1475:1493	blood urea nitrogen (BUN)	1475:1499	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	7	59	theme	p	1570:1570	arg1	<					1572:1572	p < 0.05	1570:1577	p < 0.05	1570:1577	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	6	60	theme	endurance	1194:1202	arg1	performance					1213:1223	endurance exercise performance	1194:1223	endurance exercise performance	1194:1223	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	4	61	theme	international	649:661	arg1	World					688:692	the World	684:692	the World	684:692	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	4	61	theme	international	649:661	arg1	Championships					694:706	Championships	694:706	Championships	694:706	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	4	61	theme	international	649:661	arg1	competitions					663:674	various international competitions	641:674	various international competitions such as the World Championships	641:706	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	9	62	theme	antifatigue	1971:1981	arg1	effects					1983:1989	antifatigue effects	1971:1989	antifatigue effects	1971:1989	Taken together, Tana supplementation for four weeks was effective in improving the gut microbiota, thereby enhancing exercise performance, and had antifatigue effects.
36014816	5	63	theme	male	875:878	arg1	mice					915:918	40 male Institute of Cancer Research (ICR) mice	872:918	40 male Institute of Cancer Research (ICR) mice	872:918	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	0	64	from	Effects	144:150	arg1	Mice					155:158	Mice	155:158	Mice	155:158	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	7	65	theme	biochemical	1432:1442	arg1	markers					1444:1450	fatigue-related biochemical markers	1416:1450	fatigue-related biochemical markers	1416:1450	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	4	66	from	effects	844:850	arg1	mice					855:858	mice	855:858	mice	855:858	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	5	67	theme	ICR	910:912	arg1	mice					915:918	40 male Institute of Cancer Research (ICR) mice	872:918	40 male Institute of Cancer Research (ICR) mice	872:918	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	4	68	dep	Lactiplantibacillus	542:560	arg1	plantarum					562:570	plantarum	562:570	plantarum	562:570	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	5	69	theme	mice	915:918	arg1	total					863:867	A total	861:867	A total of 40 male Institute of Cancer Research (ICR) mice	861:918	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	4	70	from	weightlifter	605:616	arg1	“Tana”					572:577	intestinal Lactiplantibacillus plantarum “Tana”	531:577	intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice	531:858	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	7	71	theme	significant	1393:1403	arg1	effects					1405:1411	significant effects	1393:1411	significant effects	1393:1411	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	5	72	theme	×	1054:1054	arg1	/mice/day					1064:1072	1.23 × 108 CFU /mice/day	1049:1072	1.23 × 108 CFU /mice/day	1049:1072	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	5	72	theme	×	1054:1054	arg1	Tana-2×					1040:1046	(3) Tana-2×	1036:1046	(3) Tana-2× (1.23 × 108 CFU /mice/day)	1036:1073	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	7	73	dep	lowered	1561:1567	arg1	<					1572:1572	p < 0.05	1570:1577	p < 0.05	1570:1577	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	8	74	theme	bacteria	1803:1810	arg1	abundance					1769:1777	abundance	1769:1777	abundance	1769:1777	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	8	74	theme	bacteria	1803:1810	arg1	ratio					1783:1787	ratio	1783:1787	ratio	1783:1787	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	1	75	from	changes	177:183	arg1	microbiota					196:205	the gut microbiota	188:205	the gut microbiota	188:205	Exercise causes changes in the gut microbiota, and in turn, the composition of the gut microbiota affects exercise performance.
36014816	7	76	theme	kinase	1521:1526	arg1	activity					1533:1540	creatine kinase (CK) activity	1512:1540	creatine kinase (CK) activity	1512:1540	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	10	77	theme	histopathological	2056:2072	arg1	damage					2074:2079	any physiological or histopathological damage	2035:2079	any physiological or histopathological damage	2035:2079	Furthermore, supplementation did not cause any physiological or histopathological damage.
36014816	2	78	theme	gut	381:383	arg1	microbiota					385:394	the gut microbiota	377:394	the gut microbiota	377:394	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	0	79	theme	Lactiplantibacillus	10:28	arg1	Tana					40:43	Probiotic Lactiplantibacillus plantarum Tana	0:43	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter	0:87	Probiotic Lactiplantibacillus plantarum Tana Isolated from an International Weightlifter Enhances Exercise Performance and Promotes Antifatigue Effects in Mice.
36014816	10	80	theme	physiological	2039:2051	arg1	damage					2074:2079	any physiological or histopathological damage	2035:2079	any physiological or histopathological damage	2035:2079	Furthermore, supplementation did not cause any physiological or histopathological damage.
36014816	8	81	theme	improved	1604:1611	arg1	dose-dependent					1678:1691	dose-dependent	1678:1691	dose-dependent	1678:1691	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	8	81	theme	improved	1604:1611	arg1	performance					1622:1632	the improved exercise performance	1600:1632	the improved exercise performance	1600:1632	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	4	82	theme	gold-medal-winning	586:603	arg1	weightlifter					605:616	a gold-medal-winning weightlifter	584:616	a gold-medal-winning weightlifter	584:616	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	4	83	theme	supplementation	767:781	arg1	benefits					750:757	the benefits	746:757	the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice	746:858	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	3	84	theme	probiotics	462:471	arg1	development					418:428	development	418:428	development	418:428	In recent years, the development and application of human-origin probiotics has gradually attracted attention.
36014816	3	84	theme	probiotics	462:471	arg1	application					434:444	application	434:444	application	434:444	In recent years, the development and application of human-origin probiotics has gradually attracted attention.
36014816	7	85	contain	had	1389:1391	arg2	effects					1405:1411	significant effects	1393:1411	significant effects	1393:1411	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	7	85	contain	had	1389:1391	arg1	supplementation					1363:1377	supplementation	1363:1377	supplementation with Tana	1363:1387	In addition, supplementation with Tana had significant effects on fatigue-related biochemical markers; lactate, ammonia, and blood urea nitrogen (BUN) levels and creatine kinase (CK) activity were significantly lowered (p < 0.05).
36014816	4	86	theme	intestinal	531:540	arg1	“Tana”					572:577	intestinal Lactiplantibacillus plantarum “Tana”	531:577	intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice	531:858	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	6	87	theme	exercise	1204:1211	arg1	performance					1213:1223	endurance exercise performance	1194:1223	endurance exercise performance	1194:1223	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	5	88	theme	CFU	1060:1062	arg1	/mice/day					1064:1072	1.23 × 108 CFU /mice/day	1049:1072	1.23 × 108 CFU /mice/day	1049:1072	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	5	88	theme	CFU	1060:1062	arg1	Tana-2×					1040:1046	(3) Tana-2×	1036:1046	(3) Tana-2× (1.23 × 108 CFU /mice/day)	1036:1073	A total of 40 male Institute of Cancer Research (ICR) mice were divided into four groups (10 mice/group): (1) vehicle (0 CFU/mice/day), (2) Tana-1× (6.15 × 107 CFU/mice/day), (3) Tana-2× (1.23 × 108 CFU /mice/day), and (4) Tana-5× (3.09 × 108 CFU/mice/day).
36014816	3	89	dep	development	418:428	arg1	the					414:416	the	414:416	the	414:416	In recent years, the development and application of human-origin probiotics has gradually attracted attention.
36014816	8	90	theme	p	1738:1738	arg1	supplementation					1721:1735	Tana supplementation	1716:1735	Tana supplementation (p < 0.05)	1716:1746	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	8	90	theme	p	1738:1738	arg1	<					1740:1740	p < 0.05	1738:1745	p < 0.05	1738:1745	We also found that the improved exercise performance and antifatigue benefits were significantly dose-dependent on increasing doses of Tana supplementation (p < 0.05), which increased the abundance and ratio of beneficial bacteria in the gut.
36014816	4	91	theme	various	641:647	arg1	World					688:692	the World	684:692	the World	684:692	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	4	91	theme	various	641:647	arg1	Championships					694:706	Championships	694:706	Championships	694:706	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	4	91	theme	various	641:647	arg1	competitions					663:674	various international competitions	641:674	various international competitions such as the World Championships	641:706	Therefore, we obtained intestinal Lactiplantibacillus plantarum “Tana” from a gold-medal-winning weightlifter, who has taken part in various international competitions such as the World Championships and the Olympic Games, to investigate the benefits of Tana supplementation for improving exercise performance and promoting antifatigue effects in mice.
36014816	2	92	theme	ways	362:365	arg1	supplementation					306:320	the supplementation	302:320	the supplementation of probiotics	302:334	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	2	92	theme	ways	362:365	arg1	ways					362:365	the most direct ways	346:365	the most direct ways	346:365	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	2	92	theme	ways	362:365	arg1	one					339:341	one	339:341	one	339:341	In addition, the supplementation of probiotics is one of the most direct ways to change the gut microbiota.
36014816	6	93	theme	vehicle	1324:1330	arg1	group					1332:1336	the vehicle group	1320:1336	the vehicle group (p < 0.05)	1320:1347	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	6	93	theme	vehicle	1324:1330	arg1	<					1341:1341	p < 0.05	1339:1346	p < 0.05	1339:1346	After four weeks of Tana supplementation, we found that the grip strength, endurance exercise performance, and glycogen storage in the liver and muscle were significantly improved compared to those in the vehicle group (p < 0.05).
36014816	1	94	theme	gut	244:246	arg1	microbiota					248:257	the gut microbiota	240:257	the gut microbiota	240:257	Exercise causes changes in the gut microbiota, and in turn, the composition of the gut microbiota affects exercise performance.
36462278	2	0	theme	analysis	527:534	arg1	accuracy					536:543	the analysis accuracy	523:543	the analysis accuracy	523:543	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	3	1	theme	native	641:646	arg1	β-CD					662:665	β-CD	662:665	β-CD	662:665	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	3	1	theme	native	641:646	arg1	α-CD					653:656	α-CD	653:656	α-CD	653:656	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	3	1	theme	native	641:646	arg1	CDs					648:650	Two native CDs	637:650	Two native CDs (α-CD and β-CD)	637:666	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	5	2	with	interaction	1099:1109	arg1	fluoroquinolones					1158:1173	various fluoroquinolones	1150:1173	various fluoroquinolones	1150:1173	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	4	3	from	determination	881:893	arg1	samples					1039:1045	urine and blood plasma samples	1016:1045	urine and blood plasma samples	1016:1045	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	7	4	theme	following	1450:1458	arg1	figures					1460:1466	The following figures	1446:1466	The following figures of merit	1446:1475	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	2	5	used	used	291:294	arg2	CDs					282:284	CDs	282:284	CDs	282:284	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	2	6	theme	substrate	585:593	arg1	components					610:619	the substrate and endogenous components	581:619	the substrate and endogenous components of body fluids	581:634	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	1	7	theme	fluoroquinolone	183:197	arg1	antibiotics					199:209	fluoroquinolone antibiotics	183:209	fluoroquinolone antibiotics	183:209	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	4	8	theme	plasma	1032:1037	arg1	samples					1039:1045	urine and blood plasma samples	1016:1045	urine and blood plasma samples	1016:1045	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	5	9	theme	various	1150:1156	arg1	fluoroquinolones					1158:1173	various fluoroquinolones	1150:1173	various fluoroquinolones	1150:1173	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	6	10	from	plasma	1424:1429	arg1	determination					1355:1367	the determination	1351:1367	the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1)	1351:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	1	11	theme	body	220:223	arg1	fluids					225:230	human body fluids	214:230	human body fluids using surface-enhanced Raman spectroscopy (SERS)	214:279	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	4	12	theme	CD-SERS	826:832	arg1	assay					834:838	The CD-SERS assay	822:838	The CD-SERS assay	822:838	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	3	13	dep	CDs	648:650	arg1	β-CD					662:665	β-CD	662:665	β-CD	662:665	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	3	13	dep	CDs	648:650	arg1	α-CD					653:656	α-CD	653:656	α-CD	653:656	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	3	13	dep	CDs	648:650	arg1	CDs					648:650	Two native CDs	637:650	Two native CDs (α-CD and β-CD)	637:666	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	6	14	theme	Calibration	1281:1291	arg1	plots					1293:1297	Calibration plots	1281:1297	Calibration plots	1281:1297	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	15	from	urine	1391:1395	arg1	determination					1355:1367	the determination	1351:1367	the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1)	1351:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	3	16	with	derivatives	685:695	arg1	groups					716:721	hydroxypropyl groups	702:721	hydroxypropyl groups	702:721	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	10	17	theme	spectrometer	2031:2042	arg1	time					1984:1987	the short analysis time	1965:1987	the short analysis time (≤15 min)	1965:1997	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	10	17	theme	spectrometer	2031:2042	arg1	use					2007:2009	the use	2003:2009	the use of a portable Raman spectrometer	2003:2042	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	10	17	theme	spectrometer	2031:2042	arg1	≤15 min					1990:1996	≤15 min	1990:1996	≤15 min	1990:1996	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	10	18	from	monitoring	2102:2111	arg1	hospitals					2116:2124	hospitals	2116:2124	hospitals	2116:2124	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	8	19	theme	concentrations	1692:1705	arg1	influence					1669:1677	the influence	1665:1677	the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal	1665:1784	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	4	20	theme	used	907:910	arg1	determination					881:893	the determination	877:893	the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples	877:1045	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	10	21	theme	analysis	1975:1982	arg1	time					1984:1987	the short analysis time	1965:1987	the short analysis time (≤15 min)	1965:1997	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	10	21	theme	analysis	1975:1982	arg1	≤15 min					1990:1996	≤15 min	1990:1996	≤15 min	1990:1996	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	5	22	theme	analyte-class-specific	1248:1269	arg1	analysis					1271:1278	the analyte-class-specific analysis	1244:1278	the analyte-class-specific analysis	1244:1278	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	7	23	theme	RSD	1535:1537	arg1	values					1539:1544	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values	1446:1544	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values	1446:1544	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	7	23	theme	RSD	1535:1537	arg1	%					1553:1553	≤15%	1550:1553	≤15%	1550:1553	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	9	24	theme	composition	1901:1911	arg1	deviation					1884:1892	the deviation	1880:1892	the deviation of the composition of the body fluid matrix	1880:1936	This step was used to evaluate possible limitations of the assay associated with the deviation of the composition of the body fluid matrix.
36462278	6	25	from	antibiotics	1376:1386	arg1	1-6 μg mL-1					1432:1442	1-6 μg mL-1	1432:1442	1-6 μg mL-1	1432:1442	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	25	from	antibiotics	1376:1386	arg1	50-500 μg mL-1					1398:1411	50-500 μg mL-1	1398:1411	50-500 μg mL-1	1398:1411	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	25	from	antibiotics	1376:1386	arg1	urine					1391:1395	urine	1391:1395	urine (50-500 μg mL-1)	1391:1412	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	25	from	antibiotics	1376:1386	arg1	plasma					1424:1429	blood plasma	1418:1429	blood plasma (1-6 μg mL-1)	1418:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	8	26	theme	endogenous	1720:1729	arg1	components					1731:1740	some main endogenous components	1710:1740	some main endogenous components of the body fluids	1710:1759	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	10	27	theme	drug	2097:2100	arg1	monitoring					2102:2111	therapeutic drug monitoring	2085:2111	therapeutic drug monitoring in hospitals	2085:2124	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	3	28	theme	best	744:747	arg1	results					749:755	the best results	740:755	the best results	740:755	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	9	29	theme	fluid	1925:1929	arg1	matrix					1931:1936	the body fluid matrix	1916:1936	the body fluid matrix	1916:1936	This step was used to evaluate possible limitations of the assay associated with the deviation of the composition of the body fluid matrix.
36462278	10	30	theme	therapeutic	2085:2095	arg1	monitoring					2102:2111	therapeutic drug monitoring	2085:2111	therapeutic drug monitoring in hospitals	2085:2124	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	2	31	dep	increase	393:400	arg1	ii					389:390	ii	389:390	ii	389:390	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	8	32	theme	body	1749:1752	arg1	fluids					1754:1759	the body fluids	1745:1759	the body fluids	1745:1759	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	2	33	theme	analyte	468:474	arg1	molecules					476:484	analyte molecules	468:484	analyte molecules	468:484	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	2	34	theme	silver	365:370	arg1	nanoparticles					372:384	silver nanoparticles	365:384	silver nanoparticles	365:384	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	6	35	theme	blood	1418:1422	arg1	1-6 μg mL-1					1432:1442	1-6 μg mL-1	1432:1442	1-6 μg mL-1	1432:1442	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	35	theme	blood	1418:1422	arg1	plasma					1424:1429	blood plasma	1418:1429	blood plasma (1-6 μg mL-1)	1418:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	2	36	theme	body	624:627	arg1	fluids					629:634	body fluids	624:634	body fluids	624:634	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	5	37	theme	SERS	1130:1133	arg1	substrate					1135:1143	the CD-modified SERS substrate	1114:1143	the CD-modified SERS substrate	1114:1143	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	4	38	theme	fluoroquinolones	937:952	arg1	determination					881:893	the determination	877:893	the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples	877:1045	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	5	39	from	difference	1081:1090	arg1	interaction					1099:1109	the interaction	1095:1109	the interaction of the CD-modified SERS substrate with various fluoroquinolones	1095:1173	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	0	40	theme	fluoroquinolone	44:58	arg1	antibiotics					60:70	fluoroquinolone antibiotics	44:70	fluoroquinolone antibiotics	44:70	Cyclodextrin-assisted SERS determination of fluoroquinolone antibiotics in urine and blood plasma.
36462278	2	41	theme	assay	425:429	arg1	sensitivity					406:416	the sensitivity	402:416	the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate	402:502	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	4	42	theme	urine	1016:1020	arg1	samples					1039:1045	urine and blood plasma samples	1016:1045	urine and blood plasma samples	1016:1045	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	7	43	theme	merit	1471:1475	arg1	figures					1460:1466	The following figures	1446:1466	The following figures of merit	1446:1475	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	4	44	theme	blood	1026:1030	arg1	samples					1039:1045	urine and blood plasma samples	1016:1045	urine and blood plasma samples	1016:1045	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	1	45	theme	surface-enhanced	238:253	arg1	SERS					275:278	SERS	275:278	SERS	275:278	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	1	45	theme	surface-enhanced	238:253	arg1	spectroscopy					261:272	surface-enhanced Raman spectroscopy	238:272	surface-enhanced Raman spectroscopy (SERS)	238:279	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	8	46	theme	analytical	1768:1777	arg1	signal					1779:1784	the analytical signal	1764:1784	the analytical signal	1764:1784	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	7	47	dep	obtained	1482:1489	arg1	respectively					1520:1531	respectively	1520:1531	respectively	1520:1531	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	5	48	used	used	1197:1200	arg2	difference					1081:1090	the non-significant difference	1061:1090	the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones	1061:1173	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	8	49	from	influence	1669:1677	arg1	signal					1779:1784	the analytical signal	1764:1784	the analytical signal	1764:1784	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	5	50	theme	non-significant	1065:1079	arg1	difference					1081:1090	the non-significant difference	1061:1090	the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones	1061:1173	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	7	51	theme	LOD	1565:1567	arg1	2.9-5.8					1580:1586	2.9-5.8	1580:1586	2.9-5.8	1580:1586	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	7	51	theme	LOD	1565:1567	arg1	values					1569:1574	LOD values	1565:1574	LOD values	1565:1574	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	9	52	theme	possible	1830:1837	arg1	limitations					1839:1849	possible limitations	1830:1849	possible limitations of the assay associated with the deviation of the composition of the body fluid matrix	1830:1936	This step was used to evaluate possible limitations of the assay associated with the deviation of the composition of the body fluid matrix.
36462278	3	53	theme	derivatives	685:695	arg1	two					672:674	two	672:674	two	672:674	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	3	53	theme	derivatives	685:695	arg1	derivatives					685:695	their derivatives	679:695	their derivatives with hydroxypropyl groups	679:721	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	6	54	theme	concentration	1317:1329	arg1	ranges					1331:1336	concentration ranges	1317:1336	concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1)	1317:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	1	55	theme	antibiotics	199:209	arg1	determination					166:178	the determination	162:178	the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS)	162:279	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	10	56	theme	Raman	2025:2029	arg1	spectrometer					2031:2042	a portable Raman spectrometer	2014:2042	a portable Raman spectrometer	2014:2042	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	2	57	theme	CD-SERS	315:321	arg1	substrate					323:331	the CD-SERS substrate	311:331	the CD-SERS substrate	311:331	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	4	58	from	samples	1039:1045	arg1	determination					881:893	the determination	877:893	the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples	877:1045	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	10	59	theme	short	1969:1973	arg1	time					1984:1987	the short analysis time	1965:1987	the short analysis time (≤15 min)	1965:1997	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	10	59	theme	short	1969:1973	arg1	≤15 min					1990:1996	≤15 min	1990:1996	≤15 min	1990:1996	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	9	60	theme	assay	1858:1862	arg1	limitations					1839:1849	possible limitations	1830:1849	possible limitations of the assay associated with the deviation of the composition of the body fluid matrix	1830:1936	This step was used to evaluate possible limitations of the assay associated with the deviation of the composition of the body fluid matrix.
36462278	5	61	theme	suitable	1231:1238	arg1	assay					1225:1229	a versatile assay	1213:1229	a versatile assay suitable for the analyte-class-specific analysis	1213:1278	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	3	62	theme	hydroxypropyl	702:714	arg1	groups					716:721	hydroxypropyl groups	702:721	hydroxypropyl groups	702:721	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	4	63	dep	used	907:910	arg1	levofloxacin					999:1010	levofloxacin	999:1010	levofloxacin	999:1010	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	4	63	dep	used	907:910	arg1	norfloxacin					970:980	norfloxacin	970:980	norfloxacin	970:980	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	4	63	dep	used	907:910	arg1	ciprofloxacin					955:967	ciprofloxacin	955:967	ciprofloxacin	955:967	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	4	63	dep	used	907:910	arg1	pefloxacin					983:992	pefloxacin	983:992	pefloxacin	983:992	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	8	64	theme	main	1715:1718	arg1	components					1731:1740	some main endogenous components	1710:1740	some main endogenous components of the body fluids	1710:1759	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	1	65	theme	cyclodextrins	131:143	arg1	use					124:126	the use	120:126	the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS)	120:279	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	10	66	theme	portable	2016:2023	arg1	spectrometer					2031:2042	a portable Raman spectrometer	2014:2042	a portable Raman spectrometer	2014:2042	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	6	67	theme	antibiotics	1376:1386	arg1	determination					1355:1367	the determination	1351:1367	the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1)	1351:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	8	68	theme	excessive	1682:1690	arg1	concentrations					1692:1705	excessive concentrations	1682:1705	excessive concentrations of some main endogenous components of the body fluids	1682:1759	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	3	69	theme	CD-SERS	776:782	arg1	substrate					784:792	CD-SERS substrate	776:792	CD-SERS substrate prepared using native β-CD	776:819	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	6	70	from	determination	1355:1367	arg1	1-6 μg mL-1					1432:1442	1-6 μg mL-1	1432:1442	1-6 μg mL-1	1432:1442	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	70	from	determination	1355:1367	arg1	50-500 μg mL-1					1398:1411	50-500 μg mL-1	1398:1411	50-500 μg mL-1	1398:1411	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	70	from	determination	1355:1367	arg1	urine					1391:1395	urine	1391:1395	urine (50-500 μg mL-1)	1391:1412	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	6	70	from	determination	1355:1367	arg1	plasma					1424:1429	blood plasma	1418:1429	blood plasma (1-6 μg mL-1)	1418:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	1	71	from	determination	166:178	arg1	fluids					225:230	human body fluids	214:230	human body fluids using surface-enhanced Raman spectroscopy (SERS)	214:279	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	5	72	theme	versatile	1215:1223	arg1	assay					1225:1229	a versatile assay	1213:1229	a versatile assay suitable for the analyte-class-specific analysis	1213:1278	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	9	73	theme	body	1920:1923	arg1	matrix					1931:1936	the body fluid matrix	1916:1936	the body fluid matrix	1916:1936	This step was used to evaluate possible limitations of the assay associated with the deviation of the composition of the body fluid matrix.
36462278	3	74	theme	native	809:814	arg1	β-CD					816:819	native β-CD	809:819	native β-CD	809:819	Two native CDs (α-CD and β-CD) and two of their derivatives with hydroxypropyl groups were tested, and the best results were obtained with CD-SERS substrate prepared using native β-CD.
36462278	2	75	theme	endogenous	599:608	arg1	components					610:619	the substrate and endogenous components	581:619	the substrate and endogenous components of body fluids	581:634	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	6	76	theme	suitable	1338:1345	arg1	ranges					1331:1336	concentration ranges	1317:1336	concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1)	1317:1443	Calibration plots were obtained for concentration ranges suitable for the determination of the antibiotics in urine (50-500 μg mL-1) and blood plasma (1-6 μg mL-1).
36462278	8	77	theme	components	1731:1740	arg1	concentrations					1692:1705	excessive concentrations	1682:1705	excessive concentrations of some main endogenous components of the body fluids	1682:1759	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	9	78	theme	matrix	1931:1936	arg1	composition					1901:1911	the composition	1897:1911	the composition of the body fluid matrix	1897:1936	This step was used to evaluate possible limitations of the assay associated with the deviation of the composition of the body fluid matrix.
36462278	2	79	theme	nanoparticles	372:384	arg1	synthesis					334:342	synthesis	334:342	synthesis	334:342	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	2	79	theme	nanoparticles	372:384	arg1	stabilization					348:360	stabilization	348:360	stabilization	348:360	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	7	80	theme	recovery	1611:1618	arg1	ranges					1620:1625	recovery ranges	1611:1625	recovery ranges	1611:1625	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	7	80	theme	recovery	1611:1618	arg1	%					1637:1637	96-105%	1631:1637	96-105%	1631:1637	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	2	81	theme	fluids	629:634	arg1	components					610:619	the substrate and endogenous components	581:619	the substrate and endogenous components of body fluids	581:634	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	2	82	dep	prepare	303:309	arg1	i					300:300	i	300:300	i	300:300	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	5	83	theme	CD-modified	1118:1128	arg1	substrate					1135:1143	the CD-modified SERS substrate	1114:1143	the CD-modified SERS substrate	1114:1143	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	8	84	theme	fluids	1754:1759	arg1	components					1731:1740	some main endogenous components	1710:1740	some main endogenous components of the body fluids	1710:1759	In addition, the influence of excessive concentrations of some main endogenous components of the body fluids on the analytical signal was studied.
36462278	0	85	theme	antibiotics	60:70	arg1	determination					27:39	determination	27:39	determination of fluoroquinolone antibiotics	27:70	Cyclodextrin-assisted SERS determination of fluoroquinolone antibiotics in urine and blood plasma.
36462278	5	86	theme	substrate	1135:1143	arg1	interaction					1099:1109	the interaction	1095:1109	the interaction of the CD-modified SERS substrate with various fluoroquinolones	1095:1173	Importantly, the non-significant difference in the interaction of the CD-modified SERS substrate with various fluoroquinolones has been successfully used to develop a versatile assay suitable for the analyte-class-specific analysis.
36462278	1	87	theme	human	214:218	arg1	fluids					225:230	human body fluids	214:230	human body fluids using surface-enhanced Raman spectroscopy (SERS)	214:279	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	0	88	dep	urine	75:79	arg1	plasma					91:96	plasma	91:96	plasma	91:96	Cyclodextrin-assisted SERS determination of fluoroquinolone antibiotics in urine and blood plasma.
36462278	9	89	used	used	1813:1816	arg2	step					1804:1807	This step	1799:1807	This step	1799:1807	This step was used to evaluate possible limitations of the assay associated with the deviation of the composition of the body fluid matrix.
36462278	4	90	theme	related	929:935	arg1	fluoroquinolones					937:952	structurally related fluoroquinolones	916:952	structurally related fluoroquinolones	916:952	The CD-SERS assay has been developed and optimized for the determination of commonly used and structurally related fluoroquinolones (ciprofloxacin, norfloxacin, pefloxacin, and levofloxacin) in urine and blood plasma samples.
36462278	7	91	dep	values	1539:1544	arg1	obtained					1482:1489	obtained	1482:1489	were obtained (for urine and blood plasma, respectively)	1477:1532	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	10	92	theme	proposed	2049:2056	arg1	assay					2058:2062	the proposed assay	2045:2062	the proposed assay	2045:2062	Therefore, accounting for the short analysis time (≤15 min) and the use of a portable Raman spectrometer, the proposed assay can be suggested for therapeutic drug monitoring in hospitals.
36462278	7	93	dep	urine	1496:1500	arg1	plasma					1512:1517	plasma	1512:1517	plasma	1512:1517	The following figures of merit were obtained (for urine and blood plasma, respectively): RSD values are ≤15% and ≤23%, LOD values are 2.9-5.8 and 0.05-0.34 μg mL-1, recovery ranges are 96-105% and 91-111%.
36462278	2	94	dep	improve	515:521	arg1	iii					510:512	iii	510:512	iii	510:512	CDs were used to (i) prepare the CD-SERS substrate (synthesis and stabilization of silver nanoparticles), (ii) increase the sensitivity of the assay by enhancing the interaction between analyte molecules and the substrate, and (iii) improve the analysis accuracy by reducing the interaction between the substrate and endogenous components of body fluids.
36462278	1	95	theme	Raman	255:259	arg1	SERS					275:278	SERS	275:278	SERS	275:278	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36462278	1	95	theme	Raman	255:259	arg1	spectroscopy					261:272	surface-enhanced Raman spectroscopy	238:272	surface-enhanced Raman spectroscopy (SERS)	238:279	This paper describes the use of cyclodextrins (CDs) to improve the determination of fluoroquinolone antibiotics in human body fluids using surface-enhanced Raman spectroscopy (SERS).
36603677	0	0	theme	decabrominated	82:95	arg1	soil					125:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	8	1	theme	high	1281:1284	arg1	efficiency					1294:1303	the high removal efficiency	1277:1303	the high removal efficiency provided by the 450°C-thermal treatment	1277:1343	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	6	2	theme	agricultural	980:991	arg1	residue					993:999	a common agricultural residue	971:999	a common agricultural residue	971:999	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	6	2	theme	agricultural	980:991	arg1	bagasse					962:968	Sugarcane bagasse	952:968	Sugarcane bagasse	952:968	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	1	3	theme	commercial	243:252	arg1	retardant					271:279	a widely used commercial brominated flame retardant	229:279	a widely used commercial brominated flame retardant	229:279	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	0	4	theme	field-weathered	66:80	arg1	soil					125:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	5	5	theme	2.47	816:819	arg1	%					820:820	%	820:820	%	820:820	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	1	6	theme	brominated	254:263	arg1	retardant					271:279	a widely used commercial brominated flame retardant	229:279	a widely used commercial brominated flame retardant	229:279	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	0	7	theme	ether-contaminated	106:123	arg1	soil					125:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	8	8	theme	recovery	1358:1365	arg1	efficiency					1367:1376	the high recovery efficiency	1349:1376	the high recovery efficiency of sugarcane bagasse	1349:1397	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	1	9	from	environment	303:313	arg1	persistent					285:294	persistent	285:294	persistent	285:294	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	1	9	from	environment	303:313	arg1	years					329:333	more than 10 years	316:333	more than 10 years	316:333	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	5	10	dep	%	820:820	arg1	to					822:823	to	822:823	to	822:823	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	1	11	theme	flame	265:269	arg1	retardant					271:279	a widely used commercial brominated flame retardant	229:279	a widely used commercial brominated flame retardant	229:279	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	0	12	theme	diphenyl	97:104	arg1	soil					125:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	field-weathered decabrominated diphenyl ether-contaminated soil	66:128	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	8	13	theme	sugarcane	1381:1389	arg1	bagasse					1391:1397	sugarcane bagasse	1381:1397	sugarcane bagasse	1381:1397	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	7	14	dep	2.73	1132:1135	arg1	to					1129:1130	to	1129:1130	to	1129:1130	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	4	15	from	removal	690:696	arg1	soil					712:715	soil	712:715	soil	712:715	In our previous study (Ko et al., 2022), we found that heating to 450 °C for 30 min achieved a complete removal of BDE-209 in soil.
36603677	4	16	theme	complete	681:688	arg1	removal					690:696	a complete removal	679:696	a complete removal of BDE-209 in soil	679:715	In our previous study (Ko et al., 2022), we found that heating to 450 °C for 30 min achieved a complete removal of BDE-209 in soil.
36603677	4	17	theme	BDE-209	701:707	arg1	removal					690:696	a complete removal	679:696	a complete removal of BDE-209 in soil	679:715	In our previous study (Ko et al., 2022), we found that heating to 450 °C for 30 min achieved a complete removal of BDE-209 in soil.
36603677	5	18	theme	soil	785:788	arg1	SOM					806:808	SOM	806:808	SOM	806:808	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	5	18	theme	soil	785:788	arg1	matter					798:803	the original soil organic matter	772:803	the original soil organic matter (SOM)	772:809	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	0	19	theme	soil	125:128	arg1	treatment					53:61	thermal treatment	45:61	thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil	45:128	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	8	20	theme	sustainable	1473:1483	arg1	remediation					1485:1495	sustainable remediation	1473:1495	sustainable remediation	1473:1495	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	5	21	theme	microbial	868:876	arg1	biomass					878:884	damaging microbial biomass	859:884	damaging microbial biomass	859:884	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	5	21	theme	microbial	868:876	arg1	texture					850:856	the soil texture	841:856	the soil texture	841:856	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	6	22	theme	common	973:978	arg1	residue					993:999	a common agricultural residue	971:999	a common agricultural residue	971:999	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	6	22	theme	common	973:978	arg1	bagasse					962:968	Sugarcane bagasse	952:968	Sugarcane bagasse	952:968	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	5	23	theme	organic	790:796	arg1	SOM					806:808	SOM	806:808	SOM	806:808	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	5	23	theme	organic	790:796	arg1	matter					798:803	the original soil organic matter	772:803	the original soil organic matter (SOM)	772:809	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	0	24	theme	thermal	4:10	arg1	degradation					12:22	thermal degradation	4:22	thermal degradation	4:22	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	8	25	theme	bagasse	1391:1397	arg1	efficiency					1367:1376	the high recovery efficiency	1349:1376	the high recovery efficiency of sugarcane bagasse	1349:1397	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	8	25	theme	bagasse	1391:1397	arg1	light					1268:1272	light	1268:1272	light of the high removal efficiency provided by the 450°C-thermal treatment	1268:1343	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	1	26	from	Taoyuan	141:147	arg1	farm					133:136	A farm	131:136	A farm at Taoyuan in Taiwan	131:157	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	3	27	theme	Thermal	528:534	arg1	treatment					536:544	Thermal treatment	528:544	Thermal treatment	528:544	Thermal treatment was studied among different approaches.
36603677	8	28	theme	promising	1452:1460	arg1	method					1462:1467	a promising method	1450:1467	a promising method for sustainable remediation	1450:1495	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	8	28	theme	promising	1452:1460	arg1	strategy					1404:1411	the strategy	1400:1411	the strategy presented in this study	1400:1435	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	1	29	theme	decabrominated	188:201	arg1	BDE-209					219:225	BDE-209	219:225	BDE-209	219:225	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	1	29	theme	decabrominated	188:201	arg1	ether					212:216	decabrominated diphenyl ether	188:216	decabrominated diphenyl ether (BDE-209)	188:226	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	1	30	from	persistent	285:294	arg1	environment					303:313	the environment	299:313	the environment	299:313	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	6	31	theme	soil	1036:1039	arg1	fertility					1041:1049	soil fertility	1036:1049	soil fertility	1036:1049	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	1	32	theme	diphenyl	203:210	arg1	BDE-209					219:225	BDE-209	219:225	BDE-209	219:225	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	1	32	theme	diphenyl	203:210	arg1	ether					212:216	decabrominated diphenyl ether	188:216	decabrominated diphenyl ether (BDE-209)	188:226	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	5	33	theme	high	731:734	arg1	temperature					736:746	the high temperature	727:746	the high temperature	727:746	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	4	34	attach	removal	690:696	arg2	BDE-209					701:707	BDE-209	701:707	BDE-209	701:707	In our previous study (Ko et al., 2022), we found that heating to 450 °C for 30 min achieved a complete removal of BDE-209 in soil.
36603677	4	34	attach	removal	690:696	arg3	soil					712:715	soil	712:715	soil	712:715	In our previous study (Ko et al., 2022), we found that heating to 450 °C for 30 min achieved a complete removal of BDE-209 in soil.
36603677	2	35	theme	soil	512:515	arg1	treatment					517:525	soil treatment	512:525	soil treatment	512:525	Since crops are able to absorb and accumulate BDE-209 from soils in our previous research, posing a hazardous risk for humans, it is essential to develop a practical method of soil treatment.
36603677	1	36	from	farm	133:136	arg1	Taiwan					152:157	Taiwan	152:157	Taiwan	152:157	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
36603677	7	37	theme	contaminated	1246:1257	arg1	soil					1259:1262	the untreated contaminated soil	1232:1262	the untreated contaminated soil	1232:1262	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	7	38	theme	bacterial	1154:1162	arg1	composition					1164:1174	its bacterial composition	1150:1174	its bacterial composition	1150:1174	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	2	39	theme	previous	408:415	arg1	research					417:424	our previous research	404:424	our previous research	404:424	Since crops are able to absorb and accumulate BDE-209 from soils in our previous research, posing a hazardous risk for humans, it is essential to develop a practical method of soil treatment.
36603677	5	40	theme	damaging	859:866	arg1	biomass					878:884	damaging microbial biomass	859:884	damaging microbial biomass	859:884	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	5	40	theme	damaging	859:866	arg1	texture					850:856	the soil texture	841:856	the soil texture	841:856	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	8	41	theme	efficiency	1294:1303	arg1	efficiency					1367:1376	the high recovery efficiency	1349:1376	the high recovery efficiency of sugarcane bagasse	1349:1397	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	8	41	theme	efficiency	1294:1303	arg1	light					1268:1272	light	1268:1272	light of the high removal efficiency provided by the 450°C-thermal treatment	1268:1343	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	5	42	theme	soil	845:848	arg1	biomass					878:884	damaging microbial biomass	859:884	damaging microbial biomass	859:884	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	5	42	theme	soil	845:848	arg1	texture					850:856	the soil texture	841:856	the soil texture	841:856	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	2	43	theme	hazardous	436:444	arg1	risk					446:449	a hazardous risk	434:449	a hazardous risk for humans	434:460	Since crops are able to absorb and accumulate BDE-209 from soils in our previous research, posing a hazardous risk for humans, it is essential to develop a practical method of soil treatment.
36603677	4	44	theme	previous	593:600	arg1	Ko					609:610	Ko	609:610	Ko	609:610	In our previous study (Ko et al., 2022), we found that heating to 450 °C for 30 min achieved a complete removal of BDE-209 in soil.
36603677	4	44	theme	previous	593:600	arg1	study					602:606	our previous study	589:606	our previous study (Ko et al., 2022)	589:624	In our previous study (Ko et al., 2022), we found that heating to 450 °C for 30 min achieved a complete removal of BDE-209 in soil.
36603677	8	45	theme	removal	1286:1292	arg1	efficiency					1294:1303	the high removal efficiency	1277:1303	the high removal efficiency provided by the 450°C-thermal treatment	1277:1343	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	6	46	theme	Sugarcane	952:960	arg1	residue					993:999	a common agricultural residue	971:999	a common agricultural residue	971:999	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	6	46	theme	Sugarcane	952:960	arg1	bagasse					962:968	Sugarcane bagasse	952:968	Sugarcane bagasse	952:968	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	6	46	theme	Sugarcane	952:960	arg1	amendment					1015:1023	an amendment	1012:1023	an amendment to restore soil fertility	1012:1049	Sugarcane bagasse, a common agricultural residue, served as an amendment to restore soil fertility.
36603677	0	47	theme	soil	28:31	arg1	recovery					33:40	soil recovery	28:40	soil recovery	28:40	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	2	48	theme	practical	492:500	arg1	method					502:507	a practical method	490:507	a practical method of soil treatment	490:525	Since crops are able to absorb and accumulate BDE-209 from soils in our previous research, posing a hazardous risk for humans, it is essential to develop a practical method of soil treatment.
36603677	3	49	theme	different	564:572	arg1	approaches					574:583	different approaches	564:583	different approaches	564:583	Thermal treatment was studied among different approaches.
36603677	5	50	theme	original	776:783	arg1	SOM					806:808	SOM	806:808	SOM	806:808	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	5	50	theme	original	776:783	arg1	matter					798:803	the original soil organic matter	772:803	the original soil organic matter (SOM)	772:809	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	0	51	theme	thermal	45:51	arg1	treatment					53:61	thermal treatment	45:61	thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil	45:128	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	7	52	theme	%	1085:1085	arg1	bagasse					1087:1093	2.5% bagasse	1082:1093	2.5% bagasse	1082:1093	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	7	53	theme	2.5	1082:1084	arg1	%					1085:1085	%	1085:1085	%	1085:1085	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	8	54	theme	450°C-thermal	1321:1333	arg1	treatment					1335:1343	the 450°C-thermal treatment	1317:1343	the 450°C-thermal treatment	1317:1343	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	2	55	from	BDE-209	382:388	arg1	research					417:424	our previous research	404:424	our previous research	404:424	Since crops are able to absorb and accumulate BDE-209 from soils in our previous research, posing a hazardous risk for humans, it is essential to develop a practical method of soil treatment.
36603677	5	56	theme	thermal	933:939	arg1	treatment					941:949	the thermal treatment	929:949	the thermal treatment	929:949	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	7	57	theme	similar	1212:1218	arg1	conditions					1201:1210	the plant growth conditions	1184:1210	the plant growth conditions similar to those of the untreated contaminated soil	1184:1262	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	7	58	theme	untreated	1236:1244	arg1	soil					1259:1262	the untreated contaminated soil	1232:1262	the untreated contaminated soil	1232:1262	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	5	59	theme	0.27	825:828	arg1	%					820:820	%	820:820	%	820:820	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	2	60	theme	treatment	517:525	arg1	method					502:507	a practical method	490:507	a practical method of soil treatment	490:525	Since crops are able to absorb and accumulate BDE-209 from soils in our previous research, posing a hazardous risk for humans, it is essential to develop a practical method of soil treatment.
36603677	0	61	dep	degradation	12:22	arg1	The					0:2	The	0:2	The	0:2	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	2	62	from	soils	395:399	arg1	BDE-209					382:388	BDE-209	382:388	BDE-209 from soils in our previous research	382:424	Since crops are able to absorb and accumulate BDE-209 from soils in our previous research, posing a hazardous risk for humans, it is essential to develop a practical method of soil treatment.
36603677	7	63	from	SOM	1111:1113	arg1	soil					1118:1121	soil	1118:1121	soil	1118:1121	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	7	64	theme	growth	1194:1199	arg1	conditions					1201:1210	the plant growth conditions	1184:1210	the plant growth conditions similar to those of the untreated contaminated soil	1184:1262	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	7	65	theme	plant	1188:1192	arg1	conditions					1201:1210	the plant growth conditions	1184:1210	the plant growth conditions similar to those of the untreated contaminated soil	1184:1262	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	0	66	theme	treatment	53:61	arg1	degradation					12:22	thermal degradation	4:22	thermal degradation	4:22	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	0	66	theme	treatment	53:61	arg1	recovery					33:40	soil recovery	28:40	soil recovery	28:40	The thermal degradation and soil recovery of thermal treatment of field-weathered decabrominated diphenyl ether-contaminated soil.
36603677	8	67	theme	high	1353:1356	arg1	efficiency					1367:1376	the high recovery efficiency	1349:1376	the high recovery efficiency of sugarcane bagasse	1349:1397	In light of the high removal efficiency provided by the 450°C-thermal treatment and the high recovery efficiency of sugarcane bagasse, the strategy presented in this study serves to be a promising method for sustainable remediation.
36603677	5	68	dep	decreased	762:770	arg1	altering					832:839	altering	832:839	altering the soil texture, damaging microbial biomass,	832:885	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	5	68	dep	decreased	762:770	arg1	affecting					896:904	affecting	896:904	thus affecting the revegetation after the thermal treatment	891:949	However, the high temperature significantly decreased the original soil organic matter (SOM) from 2.47% to 0.27%, altering the soil texture, damaging microbial biomass, and thus affecting the revegetation after the thermal treatment.
36603677	7	69	theme	Current	1052:1058	arg1	results					1060:1066	Current results	1052:1066	Current results	1052:1066	Current results indicate that 2.5% bagasse can improve the SOM in soil by up to 2.73% and restore its bacterial composition, making the plant growth conditions similar to those of the untreated contaminated soil.
36603677	1	70	theme	used	238:241	arg1	retardant					271:279	a widely used commercial brominated flame retardant	229:279	a widely used commercial brominated flame retardant	229:279	A farm at Taoyuan in Taiwan was highly contaminated with decabrominated diphenyl ether (BDE-209), a widely used commercial brominated flame retardant and persistent in the environment, more than 10 years.
37223350	0	0	theme	rare	84:87	arg1	sugars					104:109	rare bacterial-like sugars	84:109	rare bacterial-like sugars	84:109	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	7	1	theme	amoeba	1476:1481	arg1	host					1483:1486	the same amoeba host	1467:1486	the same amoeba host	1467:1486	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	2	2	theme	icosahedral	483:493	arg1	capsids					495:501	their icosahedral capsids	477:501	their icosahedral capsids	477:501	We focused on the proposed Megavirinae subfamily, for which glycan-related genes were proposed to code for proteins involved in glycosylation of the layer of fibrils surrounding their icosahedral capsids.
37223350	8	3	theme	glycobiology	1527:1538	arg1	field					1512:1516	the field	1508:1516	the field of viral glycobiology	1508:1538	This study expands the field of viral glycobiology and raises questions on how Megavirinae evolved such versatile glycosylation machinery.
37223350	0	4	contain	possess	43:49	arg2	pathways					64:71	biosynthetic pathways	51:71	biosynthetic pathways	51:71	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	0	4	contain	possess	43:49	arg1	viruses					6:12	Giant viruses	0:12	Giant viruses of the Megavirinae subfamily	0:41	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	1	5	theme	giant	165:169	arg1	proteins					186:193	proteins	186:193	proteins related to sugar synthesis and processing	186:235	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	1	5	theme	giant	165:169	arg1	discovery					150:158	The recent discovery	139:158	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing	139:235	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	1	5	theme	giant	165:169	arg1	viruses					171:177	giant viruses	165:177	giant viruses	165:177	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	8	6	theme	viral	1521:1525	arg1	glycobiology					1527:1538	viral glycobiology	1521:1538	viral glycobiology	1521:1538	This study expands the field of viral glycobiology and raises questions on how Megavirinae evolved such versatile glycosylation machinery.
37223350	4	7	theme	glycosylation	803:815	arg1	clusters					822:829	complex glycosylation gene clusters	795:829	complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases)	795:966	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	0	8	theme	bacterial-like	89:102	arg1	sugars					104:109	rare bacterial-like sugars	84:109	rare bacterial-like sugars	84:109	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	4	9	theme	biosynthetic	898:909	arg1	pathways					911:918	biosynthetic pathways	898:918	biosynthetic pathways for nucleotide-sugars and glycosyltransferases	898:965	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	4	10	theme	complex	795:801	arg1	clusters					822:829	complex glycosylation gene clusters	795:829	complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases)	795:966	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	7	11	theme	same	1437:1440	arg1	family					1442:1447	the same family	1433:1447	the same family	1433:1447	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	4	12	dep	33	853:854	arg1	to					850:851	to	850:851	to	850:851	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	3	13	theme	corresponding	539:551	arg1	pathways					566:573	corresponding biosynthetic pathways	539:573	corresponding biosynthetic pathways	539:573	We compared sugar compositions and corresponding biosynthetic pathways among clade members using a combination of chemical and bioinformatics approaches.
37223350	4	14	theme	many	722:725	arg1	aspects					727:733	many aspects	722:733	many aspects	722:733	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	7	15	theme	same	1471:1474	arg1	host					1483:1486	the same amoeba host	1467:1486	the same amoeba host	1467:1486	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	7	16	from	existence	1294:1302	arg1	family					1339:1344	this family	1334:1344	this family	1334:1344	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	3	17	theme	biosynthetic	553:564	arg1	pathways					566:573	corresponding biosynthetic pathways	539:573	corresponding biosynthetic pathways	539:573	We compared sugar compositions and corresponding biosynthetic pathways among clade members using a combination of chemical and bioinformatics approaches.
37223350	7	18	theme	advantageous	1365:1376	arg1	strategy					1378:1385	an advantageous strategy	1362:1385	an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host	1362:1486	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	2	19	theme	layer	448:452	arg1	glycosylation					427:439	glycosylation	427:439	glycosylation of the layer of fibrils surrounding their icosahedral capsids	427:501	We focused on the proposed Megavirinae subfamily, for which glycan-related genes were proposed to code for proteins involved in glycosylation of the layer of fibrils surrounding their icosahedral capsids.
37223350	6	20	theme	key	1213:1215	arg1	features					1217:1224	key features	1213:1224	key features	1213:1224	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	4	21	theme	fibril	882:887	arg1	glycans					889:895	their fibril glycans	876:895	their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases)	876:966	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	5	22	theme	rare	993:996	arg1	amino-sugars					998:1009	rare amino-sugars	993:1009	rare amino-sugars	993:1009	Second, they synthesize rare amino-sugars, usually restricted to bacteria and absent from their eukaryotic host.
37223350	3	23	dep	chemical	618:625	arg1	approaches					646:655	approaches	646:655	approaches	646:655	We compared sugar compositions and corresponding biosynthetic pathways among clade members using a combination of chemical and bioinformatics approaches.
37223350	0	24	theme	Giant	0:4	arg1	viruses					6:12	Giant viruses	0:12	Giant viruses of the Megavirinae subfamily	0:41	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	6	25	theme	clade	1253:1257	arg1	E					1259:1259	clade E	1253:1259	clade E	1253:1259	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	6	25	theme	clade	1253:1257	arg1	japonicus					1242:1250	Cotonvirus japonicus	1231:1250	Cotonvirus japonicus (clade E)	1231:1260	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	7	26	theme	family	1442:1447	arg1	members					1422:1428	members	1422:1428	members of the same family	1422:1447	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	5	27	from	host	1076:1079	arg1	absent					1047:1052	absent	1047:1052	absent	1047:1052	Second, they synthesize rare amino-sugars, usually restricted to bacteria and absent from their eukaryotic host.
37223350	1	28	theme	related	195:201	arg1	proteins					186:193	proteins	186:193	proteins related to sugar synthesis and processing	186:235	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	1	28	theme	related	195:201	arg1	viruses					171:177	giant viruses	165:177	giant viruses	165:177	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	1	29	theme	glycosylation	274:286	arg1	machinery					288:296	their glycosylation machinery	268:296	their glycosylation machinery	268:296	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	3	30	theme	clade	581:585	arg1	members					587:593	clade members	581:593	clade members	581:593	We compared sugar compositions and corresponding biosynthetic pathways among clade members using a combination of chemical and bioinformatics approaches.
37223350	6	31	theme	clade	1280:1284	arg1	Tupanviruses					1266:1277	Tupanviruses	1266:1277	Tupanviruses (clade D)	1266:1287	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	6	31	theme	clade	1280:1284	arg1	D					1286:1286	clade D	1280:1286	clade D	1280:1286	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	1	32	theme	machinery	288:296	arg1	study					259:263	the study	255:263	the study of their glycosylation machinery	255:296	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	0	33	theme	clade-specific	116:129	arg1	manner					131:136	a clade-specific manner	114:136	a clade-specific manner	114:136	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	3	34	theme	sugar	516:520	arg1	compositions					522:533	sugar compositions	516:533	sugar compositions	516:533	We compared sugar compositions and corresponding biosynthetic pathways among clade members using a combination of chemical and bioinformatics approaches.
37223350	1	35	theme	sugar	206:210	arg1	synthesis					212:220	sugar synthesis	206:220	sugar synthesis	206:220	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	0	36	theme	subfamily	33:41	arg1	viruses					6:12	Giant viruses	0:12	Giant viruses of the Megavirinae subfamily	0:41	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	7	37	theme	toolbox	1323:1329	arg1	existence					1294:1302	The existence	1290:1302	The existence of a glycosylation toolbox in this family	1290:1344	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	3	38	theme	chemical	618:625	arg1	combination					603:613	a combination	601:613	a combination of chemical and bioinformatics approaches	601:655	We compared sugar compositions and corresponding biosynthetic pathways among clade members using a combination of chemical and bioinformatics approaches.
37223350	4	39	theme	Megavirinae	685:695	arg1	glycosylation					697:709	Megavirinae glycosylation	685:709	Megavirinae glycosylation	685:709	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	0	40	theme	Megavirinae	21:31	arg1	subfamily					33:41	the Megavirinae subfamily	17:41	the Megavirinae subfamily	17:41	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	8	41	theme	glycosylation	1603:1615	arg1	machinery					1617:1625	versatile glycosylation machinery	1593:1625	versatile glycosylation machinery	1593:1625	This study expands the field of viral glycobiology and raises questions on how Megavirinae evolved such versatile glycosylation machinery.
37223350	5	42	theme	eukaryotic	1065:1074	arg1	host					1076:1079	their eukaryotic host	1059:1079	their eukaryotic host	1059:1079	Second, they synthesize rare amino-sugars, usually restricted to bacteria and absent from their eukaryotic host.
37223350	6	43	theme	Megavirinae	1106:1116	arg1	glycosylation					1118:1130	Megavirinae glycosylation	1106:1130	Megavirinae glycosylation	1106:1130	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	2	44	theme	fibrils	457:463	arg1	layer					448:452	the layer	444:452	the layer of fibrils surrounding their icosahedral capsids	444:501	We focused on the proposed Megavirinae subfamily, for which glycan-related genes were proposed to code for proteins involved in glycosylation of the layer of fibrils surrounding their icosahedral capsids.
37223350	8	45	theme	versatile	1593:1601	arg1	machinery					1617:1625	versatile glycosylation machinery	1593:1625	versatile glycosylation machinery	1593:1625	This study expands the field of viral glycobiology and raises questions on how Megavirinae evolved such versatile glycosylation machinery.
37223350	4	46	contain	have	790:793	arg1	they					785:788	they	785:788	they	785:788	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	4	46	contain	have	790:793	arg2	clusters					822:829	complex glycosylation gene clusters	795:829	complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases)	795:966	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	6	47	theme	Moumouvirus	1159:1169	arg1	outsider					1196:1203	a B-clade outsider	1186:1203	a B-clade outsider	1186:1203	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	6	47	theme	Moumouvirus	1159:1169	arg1	australiensis					1171:1183	Moumouvirus australiensis	1159:1183	Moumouvirus australiensis	1159:1183	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	6	48	theme	B-clade	1188:1194	arg1	outsider					1196:1203	a B-clade outsider	1186:1203	a B-clade outsider	1186:1203	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	6	48	theme	B-clade	1188:1194	arg1	australiensis					1171:1183	Moumouvirus australiensis	1159:1183	Moumouvirus australiensis	1159:1183	Finally, we showed that Megavirinae glycosylation is clade-specific and that Moumouvirus australiensis, a B-clade outsider, shares key features with Cotonvirus japonicus (clade E) and Tupanviruses (clade D).
37223350	4	49	dep	glycans	889:895	arg1	pathways					911:918	biosynthetic pathways	898:918	biosynthetic pathways for nucleotide-sugars and glycosyltransferases	898:965	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	5	50	dep	synthesize	982:991	arg1	absent					1047:1052	absent	1047:1052	absent	1047:1052	Second, they synthesize rare amino-sugars, usually restricted to bacteria and absent from their eukaryotic host.
37223350	5	50	dep	synthesize	982:991	arg1	restricted					1020:1029	restricted	1020:1029	restricted	1020:1029	Second, they synthesize rare amino-sugars, usually restricted to bacteria and absent from their eukaryotic host.
37223350	7	51	theme	glycosylation	1309:1321	arg1	toolbox					1323:1329	a glycosylation toolbox	1307:1329	a glycosylation toolbox	1307:1329	The existence of a glycosylation toolbox in this family could represent an advantageous strategy to survive in an environment where members of the same family are competing for the same amoeba host.
37223350	2	52	theme	Megavirinae	326:336	arg1	subfamily					338:346	the proposed Megavirinae subfamily	313:346	the proposed Megavirinae subfamily	313:346	We focused on the proposed Megavirinae subfamily, for which glycan-related genes were proposed to code for proteins involved in glycosylation of the layer of fibrils surrounding their icosahedral capsids.
37223350	3	53	theme	bioinformatics	631:644	arg1	combination					603:613	a combination	601:613	a combination of chemical and bioinformatics approaches	601:655	We compared sugar compositions and corresponding biosynthetic pathways among clade members using a combination of chemical and bioinformatics approaches.
37223350	4	54	theme	gene	817:820	arg1	clusters					822:829	complex glycosylation gene clusters	795:829	complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases)	795:966	We first demonstrated that Megavirinae glycosylation differs in many aspects from what was previously reported for viruses, as they have complex glycosylation gene clusters made of six and up to 33 genes to synthetize their fibril glycans (biosynthetic pathways for nucleotide-sugars and glycosyltransferases).
37223350	2	55	theme	proposed	317:324	arg1	subfamily					338:346	the proposed Megavirinae subfamily	313:346	the proposed Megavirinae subfamily	313:346	We focused on the proposed Megavirinae subfamily, for which glycan-related genes were proposed to code for proteins involved in glycosylation of the layer of fibrils surrounding their icosahedral capsids.
37223350	2	56	gly	glycosylation	427:439	arg1	layer					448:452	the layer	444:452	the layer of fibrils surrounding their icosahedral capsids	444:501	We focused on the proposed Megavirinae subfamily, for which glycan-related genes were proposed to code for proteins involved in glycosylation of the layer of fibrils surrounding their icosahedral capsids.
37223350	1	57	theme	recent	143:148	arg1	discovery					150:158	The recent discovery	139:158	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing	139:235	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	1	57	theme	recent	143:148	arg1	viruses					171:177	giant viruses	165:177	giant viruses	165:177	The recent discovery that giant viruses encode proteins related to sugar synthesis and processing paved the way for the study of their glycosylation machinery.
37223350	0	58	theme	biosynthetic	51:62	arg1	pathways					64:71	biosynthetic pathways	51:71	biosynthetic pathways	51:71	Giant viruses of the Megavirinae subfamily possess biosynthetic pathways to produce rare bacterial-like sugars in a clade-specific manner.
37223350	2	59	theme	glycan-related	359:372	arg1	genes					374:378	glycan-related genes	359:378	glycan-related genes	359:378	We focused on the proposed Megavirinae subfamily, for which glycan-related genes were proposed to code for proteins involved in glycosylation of the layer of fibrils surrounding their icosahedral capsids.
36266410	12	0	theme	HA	1658:1659	arg1	concentrations					1661:1674	The total HA concentrations	1648:1674	The total HA concentrations	1648:1674	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	12	0	theme	HA	1658:1659	arg1	lower					1686:1690	lower	1686:1690	lower	1686:1690	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	10	1	theme	linear	1458:1463	arg1	count					1472:1476	count	1472:1476	count	1472:1476	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	10	1	theme	linear	1458:1463	arg1	model					1465:1469	generalized linear model	1446:1469	generalized linear model	1446:1469	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	11	2	theme	total	1563:1567	arg1	count					1572:1576	the OA grade and total EV count	1546:1576	count	1572:1576	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	8	3	theme	larger	1149:1154	arg1	EVs					1156:1158	larger EVs	1149:1158	larger EVs	1149:1158	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	14	4	from	potential	1904:1912	arg1	pathogenesis					1875:1886	the pathogenesis	1871:1886	the pathogenesis of OA	1871:1892	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	4	5	theme	horse-a	615:621	arg1	model					642:646	the horse-a well-recognized OA model	611:646	the horse-a well-recognized OA model	611:646	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	12	6	dep	model	1718:1722	arg1	p = 0.002					1725:1733	p = 0.002	1725:1733	p = 0.002	1725:1733	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	2	7	theme	synovial	294:301	arg1	cells					309:313	synovial joint cells	294:313	synovial joint cells	294:313	Extracellular vesicles (EVs) released by synovial joint cells can induce both pro- and anti-OA effects.
36266410	4	8	theme	OA	639:640	arg1	model					642:646	the horse-a well-recognized OA model	611:646	the horse-a well-recognized OA model	611:646	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	13	9	theme	SF	1791:1792	arg1	HA-EVs					1805:1810	HA-EVs	1805:1810	HA-EVs	1805:1810	To conclude, the present study discovered a potential SF biomarker (HA-EVs) for naturally occurring equine OA.
36266410	13	9	theme	SF	1791:1792	arg1	biomarker					1794:1802	a potential SF biomarker	1779:1802	a potential SF biomarker (HA-EVs) for naturally occurring equine OA	1779:1845	To conclude, the present study discovered a potential SF biomarker (HA-EVs) for naturally occurring equine OA.
36266410	2	10	theme	pro-	331:334	arg1	effects					348:354	both pro- and anti-OA effects	326:354	both pro- and anti-OA effects	326:354	Extracellular vesicles (EVs) released by synovial joint cells can induce both pro- and anti-OA effects.
36266410	8	11	theme	nanoparticle	994:1005	arg1	NTA					1026:1028	NTA	1026:1028	NTA	1026:1028	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	8	11	theme	nanoparticle	994:1005	arg1	analysis					1016:1023	nanoparticle tracking analysis	994:1023	nanoparticle tracking analysis (NTA)	994:1029	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	11	12	dep	was	1511:1513	arg1	- 0.327					1621:1627	- 0.327	1621:1627	- 0.327	1621:1627	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	4	13	theme	early	595:599	arg1	stages					601:606	early stages	595:606	early stages in the horse-a well-recognized OA model	595:646	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	9	14	theme	small	1197:1201	arg1	EVs					1203:1205	small EVs	1197:1205	small EVs measured by NTA	1197:1221	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	14	15	theme	future	1974:1979	arg1	studies					1981:1987	future studies	1974:1987	future studies	1974:1987	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	5	16	theme	SF	677:678	arg1	EVs					680:682	SF EVs	677:682	SF EVs	677:682	The aim was to characterize SF EVs and their HA cargo in 19 horses.
36266410	5	17	from	EVs	680:682	arg1	horses					709:714	19 horses	706:714	19 horses	706:714	The aim was to characterize SF EVs and their HA cargo in 19 horses.
36266410	13	18	theme	present	1754:1760	arg1	study					1762:1766	the present study	1750:1766	the present study	1750:1766	To conclude, the present study discovered a potential SF biomarker (HA-EVs) for naturally occurring equine OA.
36266410	5	19	from	cargo	697:701	arg1	horses					709:714	19 horses	706:714	19 horses	706:714	The aim was to characterize SF EVs and their HA cargo in 19 horses.
36266410	1	20	theme	degenerative	140:151	arg1	disease					159:165	a degenerative joint disease	138:165	a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations	138:250	Osteoarthritis (OA) is a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations.
36266410	1	20	theme	degenerative	140:151	arg1	Osteoarthritis					115:128	Osteoarthritis	115:128	Osteoarthritis (OA)	115:133	Osteoarthritis (OA) is a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations.
36266410	7	21	theme	size-exclusion	931:944	arg1	chromatography					946:959	size-exclusion chromatography	931:959	size-exclusion chromatography	931:959	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
36266410	4	22	theme	altered	494:500	arg1	counts					505:510	altered EV counts	494:510	altered EV counts	494:510	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	14	23	theme	therapeutic	1947:1957	arg1	target					1959:1964	therapeutic target	1947:1964	therapeutic target	1947:1964	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	7	24	link	enzyme-linked	899:911	arg1	assay					921:925	a sandwich-type enzyme-linked sorbent assay	883:925	a sandwich-type enzyme-linked sorbent assay	883:925	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
36266410	0	25	theme	equine	92:97	arg1	osteoarthritis					99:112	naturally occurring equine osteoarthritis	72:112	naturally occurring equine osteoarthritis	72:112	Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.
36266410	1	26	with	disease	159:165	arg1	pathogenesis					196:207	inadequately understood pathogenesis	172:207	inadequately understood pathogenesis leading to pain and functional limitations	172:250	Osteoarthritis (OA) is a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations.
36266410	7	27	theme	enzyme-linked	899:911	arg1	assay					921:925	a sandwich-type enzyme-linked sorbent assay	883:925	a sandwich-type enzyme-linked sorbent assay	883:925	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
36266410	14	28	theme	joint	1919:1923	arg1	biomarker					1933:1941	a joint disease biomarker	1917:1941	a joint disease biomarker	1917:1941	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	5	29	theme	HA	694:695	arg1	cargo					697:701	their HA cargo	688:701	their HA cargo	688:701	The aim was to characterize SF EVs and their HA cargo in 19 horses.
36266410	10	30	theme	HA-containing	1399:1411	arg1	HA-EVs					1418:1423	larger HA-containing EVs (HA-EVs)	1392:1424	larger HA-containing EVs (HA-EVs)	1392:1424	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	12	31	dep	lower	1686:1690	arg1	model					1718:1722	generalized linear model	1699:1722	generalized linear model	1699:1722	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	0	32	theme	hyaluronic	10:19	arg1	vesicles					51:58	hyaluronic acid-containing extracellular vesicles	10:58	hyaluronic acid-containing extracellular vesicles	10:58	Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.
36266410	8	33	theme	small	1057:1061	arg1	EVs					1063:1065	small EVs	1057:1065	small EVs	1057:1065	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	4	34	theme	synovial	550:557	arg1	SF					566:567	SF	566:567	SF	566:567	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	4	34	theme	synovial	550:557	arg1	fluid					559:563	OA synovial fluid	547:563	OA synovial fluid (SF)	547:568	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	14	35	theme	OA	1891:1892	arg1	pathogenesis					1875:1886	the pathogenesis	1871:1886	the pathogenesis of OA	1871:1892	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	3	36	theme	molecules	453:461	arg1	one					432:434	one	432:434	one	432:434	Hyaluronic acid (HA) lubricates the surfaces of articular cartilage and is one of the bioactive molecules transported by EVs.
36266410	3	36	theme	molecules	453:461	arg1	molecules					453:461	the bioactive molecules	439:461	the bioactive molecules transported by EVs	439:480	Hyaluronic acid (HA) lubricates the surfaces of articular cartilage and is one of the bioactive molecules transported by EVs.
36266410	10	37	dep	number	1367:1372	arg1	the					1363:1365	the	1363:1365	the	1363:1365	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	9	38	theme	hyaluronidase	1294:1306	arg1	pre-treatment					1308:1320	hyaluronidase pre-treatment	1294:1320	hyaluronidase pre-treatment of the samples	1294:1335	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	0	39	theme	extracellular	37:49	arg1	vesicles					51:58	hyaluronic acid-containing extracellular vesicles	10:58	hyaluronic acid-containing extracellular vesicles	10:58	Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.
36266410	6	40	theme	metacarpophalangeal	787:805	arg1	joints					807:812	contralateral metacarpophalangeal joints	773:812	contralateral metacarpophalangeal joints	773:812	SF was collected after euthanasia from control, OA, and contralateral metacarpophalangeal joints.
36266410	3	41	theme	articular	405:413	arg1	cartilage					415:423	articular cartilage	405:423	articular cartilage	405:423	Hyaluronic acid (HA) lubricates the surfaces of articular cartilage and is one of the bioactive molecules transported by EVs.
36266410	10	42	theme	generalized	1446:1456	arg1	count					1472:1476	count	1472:1476	count	1472:1476	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	10	42	theme	generalized	1446:1456	arg1	model					1465:1469	generalized linear model	1446:1469	generalized linear model	1446:1469	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	8	43	theme	confocal	1074:1081	arg1	CLSM					1110:1113	CLSM	1110:1113	CLSM	1110:1113	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	8	43	theme	confocal	1074:1081	arg1	microscopy					1098:1107	confocal laser scanning microscopy	1074:1107	confocal laser scanning microscopy (CLSM)	1074:1114	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	9	44	theme	samples	1329:1335	arg1	pre-treatment					1308:1320	hyaluronidase pre-treatment	1294:1320	hyaluronidase pre-treatment of the samples	1294:1335	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	12	45	theme	generalized	1699:1709	arg1	model					1718:1722	generalized linear model	1699:1722	generalized linear model	1699:1722	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	12	46	theme	total	1652:1656	arg1	concentrations					1661:1674	The total HA concentrations	1648:1674	The total HA concentrations	1648:1674	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	12	46	theme	total	1652:1656	arg1	lower					1686:1690	lower	1686:1690	lower	1686:1690	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	11	47	theme	inverse	1518:1524	arg1	association					1526:1536	an inverse association	1515:1536	an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV %	1515:1602	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	7	48	theme	size	844:847	arg1	distribution					849:860	size distribution	844:860	size distribution	844:860	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
36266410	11	49	theme	OA	1550:1551	arg1	grade					1553:1557	the OA grade and total EV count	1546:1576	grade	1553:1557	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	13	50	theme	potential	1781:1789	arg1	HA-EVs					1805:1810	HA-EVs	1805:1810	HA-EVs	1805:1810	To conclude, the present study discovered a potential SF biomarker (HA-EVs) for naturally occurring equine OA.
36266410	13	50	theme	potential	1781:1789	arg1	biomarker					1794:1802	a potential SF biomarker	1779:1802	a potential SF biomarker (HA-EVs) for naturally occurring equine OA	1779:1845	To conclude, the present study discovered a potential SF biomarker (HA-EVs) for naturally occurring equine OA.
36266410	7	51	theme	SF	819:820	arg1	concentrations					825:838	The SF HA concentrations	815:838	The SF HA concentrations	815:838	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
36266410	8	52	theme	image	1120:1124	arg1	analysis					1126:1133	image analysis	1120:1133	image analysis	1120:1133	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	14	53	theme	HA-EVs	1861:1866	arg1	potential					1904:1912	their potential	1898:1912	their potential as a joint disease biomarker and therapeutic target	1898:1964	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	14	53	theme	HA-EVs	1861:1866	arg1	roles					1852:1856	The roles	1848:1856	The roles of HA-EVs in the pathogenesis of OA	1848:1892	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	11	54	theme	EV	1569:1570	arg1	count					1572:1576	the OA grade and total EV count	1546:1576	count	1572:1576	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	2	55	theme	joint	303:307	arg1	cells					309:313	synovial joint cells	294:313	synovial joint cells	294:313	Extracellular vesicles (EVs) released by synovial joint cells can induce both pro- and anti-OA effects.
36266410	1	56	theme	understood	185:194	arg1	pathogenesis					196:207	inadequately understood pathogenesis	172:207	inadequately understood pathogenesis leading to pain and functional limitations	172:250	Osteoarthritis (OA) is a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations.
36266410	9	57	theme	size	1176:1179	arg1	distribution					1181:1192	size distribution	1176:1192	size distribution	1176:1192	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	8	58	theme	tracking	1007:1014	arg1	NTA					1026:1028	NTA	1026:1028	NTA	1026:1028	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	8	58	theme	tracking	1007:1014	arg1	analysis					1016:1023	nanoparticle tracking analysis	994:1023	nanoparticle tracking analysis (NTA)	994:1029	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	4	59	theme	well-recognized	623:637	arg1	model					642:646	the horse-a well-recognized OA model	611:646	the horse-a well-recognized OA model	611:646	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	4	60	from	stages	601:606	arg1	model					642:646	the horse-a well-recognized OA model	611:646	the horse-a well-recognized OA model	611:646	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	10	61	dep	%	1490:1490	arg1	p = 0.028					1493:1501	p = 0.028	1493:1501	p = 0.028	1493:1501	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	9	62	theme	EVs	1203:1205	arg1	number					1165:1170	number	1165:1170	number	1165:1170	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	9	62	theme	EVs	1203:1205	arg1	distribution					1181:1192	size distribution	1176:1192	size distribution	1176:1192	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	1	63	theme	functional	229:238	arg1	limitations					240:250	functional limitations	229:250	functional limitations	229:250	Osteoarthritis (OA) is a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations.
36266410	10	64	dep	model	1465:1469	arg1	p = 0.024					1479:1487	p = 0.024	1479:1487	p = 0.024	1479:1487	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	10	64	dep	model	1465:1469	arg1	%					1490:1490	%	1490:1490	%: p = 0.028	1490:1501	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	11	65	dep	- 0.327	1621:1627	arg1	to					1618:1619	to	1618:1619	to	1618:1619	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	11	65	dep	- 0.327	1621:1627	arg1	p = 0.012-0.045					1630:1644	p = 0.012-0.045	1630:1644	p = 0.012-0.045	1630:1644	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	2	66	theme	Extracellular	253:265	arg1	vesicles					267:274	Extracellular vesicles	253:274	Extracellular vesicles (EVs) released by synovial joint cells	253:313	Extracellular vesicles (EVs) released by synovial joint cells can induce both pro- and anti-OA effects.
36266410	2	66	theme	Extracellular	253:265	arg1	EVs					277:279	EVs	277:279	EVs	277:279	Extracellular vesicles (EVs) released by synovial joint cells can induce both pro- and anti-OA effects.
36266410	9	67	dep	number	1165:1170	arg1	The					1161:1163	The	1161:1163	The	1161:1163	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	0	68	theme	occurring	82:90	arg1	osteoarthritis					99:112	naturally occurring equine osteoarthritis	72:112	naturally occurring equine osteoarthritis	72:112	Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.
36266410	1	69	theme	joint	153:157	arg1	disease					159:165	a degenerative joint disease	138:165	a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations	138:250	Osteoarthritis (OA) is a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations.
36266410	1	69	theme	joint	153:157	arg1	Osteoarthritis					115:128	Osteoarthritis	115:128	Osteoarthritis (OA)	115:133	Osteoarthritis (OA) is a degenerative joint disease with inadequately understood pathogenesis leading to pain and functional limitations.
36266410	3	70	theme	Hyaluronic	357:366	arg1	HA					374:375	HA	374:375	HA	374:375	Hyaluronic acid (HA) lubricates the surfaces of articular cartilage and is one of the bioactive molecules transported by EVs.
36266410	3	70	theme	Hyaluronic	357:366	arg1	acid					368:371	Hyaluronic acid	357:371	Hyaluronic acid (HA)	357:376	Hyaluronic acid (HA) lubricates the surfaces of articular cartilage and is one of the bioactive molecules transported by EVs.
36266410	4	71	theme	EV	502:503	arg1	counts					505:510	altered EV counts	494:510	altered EV counts	494:510	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	8	72	theme	scanning	1089:1096	arg1	CLSM					1110:1113	CLSM	1110:1113	CLSM	1110:1113	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	8	72	theme	scanning	1089:1096	arg1	microscopy					1098:1107	confocal laser scanning microscopy	1074:1107	confocal laser scanning microscopy (CLSM)	1074:1114	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	14	73	theme	disease	1925:1931	arg1	biomarker					1933:1941	a joint disease biomarker	1917:1941	a joint disease biomarker	1917:1941	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	7	74	theme	sorbent	913:919	arg1	assay					921:925	a sandwich-type enzyme-linked sorbent assay	883:925	a sandwich-type enzyme-linked sorbent assay	883:925	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
36266410	8	75	used	utilized	1036:1043	arg2	Ultracentrifugation					962:980	Ultracentrifugation	962:980	Ultracentrifugation followed by nanoparticle tracking analysis (NTA)	962:1029	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	10	76	theme	EVs	1413:1415	arg1	HA-EVs					1418:1423	larger HA-containing EVs (HA-EVs)	1392:1424	larger HA-containing EVs (HA-EVs)	1392:1424	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	3	77	theme	bioactive	443:451	arg1	molecules					453:461	the bioactive molecules	439:461	the bioactive molecules transported by EVs	439:480	Hyaluronic acid (HA) lubricates the surfaces of articular cartilage and is one of the bioactive molecules transported by EVs.
36266410	7	78	theme	sandwich-type	885:897	arg1	assay					921:925	a sandwich-type enzyme-linked sorbent assay	883:925	a sandwich-type enzyme-linked sorbent assay	883:925	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
36266410	4	79	theme	OA	547:548	arg1	SF					566:567	SF	566:567	SF	566:567	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	4	79	theme	OA	547:548	arg1	fluid					559:563	OA synovial fluid	547:563	OA synovial fluid (SF)	547:568	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	10	80	theme	larger	1392:1397	arg1	HA-EVs					1418:1423	larger HA-containing EVs (HA-EVs)	1392:1424	larger HA-containing EVs (HA-EVs)	1392:1424	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	0	81	theme	acid-containing	21:35	arg1	vesicles					51:58	hyaluronic acid-containing extracellular vesicles	10:58	hyaluronic acid-containing extracellular vesicles	10:58	Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.
36266410	14	82	from	roles	1852:1856	arg1	pathogenesis					1875:1886	the pathogenesis	1871:1886	the pathogenesis of OA	1871:1892	The roles of HA-EVs in the pathogenesis of OA and their potential as a joint disease biomarker and therapeutic target warrant future studies.
36266410	13	83	theme	occurring	1827:1835	arg1	OA					1844:1845	naturally occurring equine OA	1817:1845	naturally occurring equine OA	1817:1845	To conclude, the present study discovered a potential SF biomarker (HA-EVs) for naturally occurring equine OA.
36266410	11	84	theme	HA-EV	1579:1583	arg1	count					1585:1589	HA-EV count	1579:1589	HA-EV count	1579:1589	There was an inverse association between the OA grade and total EV count, HA-EV count, and HA-EV % (rs = - 0.264 to - 0.327, p = 0.012-0.045).
36266410	4	85	located	observed	535:542	arg1	humans					486:491	humans	486:491	humans	486:491	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	4	85	located	observed	535:542	arg2	composition					516:526	composition	516:526	composition	516:526	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	4	85	located	observed	535:542	arg2	counts					505:510	altered EV counts	494:510	altered EV counts	494:510	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	4	85	located	observed	535:542	arg1	SF					566:567	SF	566:567	SF	566:567	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	4	85	located	observed	535:542	arg1	fluid					559:563	OA synovial fluid	547:563	OA synovial fluid (SF)	547:568	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	0	86	theme	vesicles	51:58	arg1	Counts					0:5	Counts	0:5	Counts of hyaluronic acid-containing extracellular vesicles	0:58	Counts of hyaluronic acid-containing extracellular vesicles decrease in naturally occurring equine osteoarthritis.
36266410	10	87	theme	HA-EVs	1418:1423	arg1	proportion					1378:1387	proportion	1378:1387	proportion	1378:1387	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	10	87	theme	HA-EVs	1418:1423	arg1	number					1367:1372	number	1367:1372	number	1367:1372	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	10	88	dep	decreased	1426:1434	arg1	count					1472:1476	count	1472:1476	count	1472:1476	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	10	88	dep	decreased	1426:1434	arg1	model					1465:1469	generalized linear model	1446:1469	generalized linear model	1446:1469	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	9	89	theme	pre-treatment	1308:1320	arg1	lack					1286:1289	the lack	1282:1289	the lack of hyaluronidase pre-treatment of the samples	1282:1335	The number and size distribution of small EVs measured by NTA were unaffected by OA, but these results may be limited by the lack of hyaluronidase pre-treatment of the samples.
36266410	8	90	theme	laser	1083:1087	arg1	CLSM					1110:1113	CLSM	1110:1113	CLSM	1110:1113	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	8	90	theme	laser	1083:1087	arg1	microscopy					1098:1107	confocal laser scanning microscopy	1074:1107	confocal laser scanning microscopy (CLSM)	1074:1114	Ultracentrifugation followed by nanoparticle tracking analysis (NTA) were utilized to quantify small EVs, while confocal laser scanning microscopy (CLSM) and image analysis characterized larger EVs.
36266410	6	91	theme	contralateral	773:785	arg1	joints					807:812	contralateral metacarpophalangeal joints	773:812	contralateral metacarpophalangeal joints	773:812	SF was collected after euthanasia from control, OA, and contralateral metacarpophalangeal joints.
36266410	3	92	theme	cartilage	415:423	arg1	surfaces					393:400	the surfaces	389:400	the surfaces of articular cartilage	389:423	Hyaluronic acid (HA) lubricates the surfaces of articular cartilage and is one of the bioactive molecules transported by EVs.
36266410	12	93	theme	linear	1711:1716	arg1	model					1718:1722	generalized linear model	1699:1722	generalized linear model	1699:1722	The total HA concentrations were also lower in OA (generalized linear model, p = 0.002).
36266410	4	94	theme	EV	577:578	arg1	research					580:587	EV research	577:587	EV research	577:587	In humans, altered EV counts and composition can be observed in OA synovial fluid (SF), while EV research is in early stages in the horse-a well-recognized OA model.
36266410	10	95	theme	OA	1439:1440	arg1	SF					1442:1443	OA SF	1439:1443	OA SF	1439:1443	When visualized by CLSM, the number and proportion of larger HA-containing EVs (HA-EVs) decreased in OA SF (generalized linear model, count: p = 0.024, %: p = 0.028).
36266410	2	96	theme	anti-OA	340:346	arg1	effects					348:354	both pro- and anti-OA effects	326:354	both pro- and anti-OA effects	326:354	Extracellular vesicles (EVs) released by synovial joint cells can induce both pro- and anti-OA effects.
36266410	13	97	theme	equine	1837:1842	arg1	OA					1844:1845	naturally occurring equine OA	1817:1845	naturally occurring equine OA	1817:1845	To conclude, the present study discovered a potential SF biomarker (HA-EVs) for naturally occurring equine OA.
36266410	7	98	theme	HA	822:823	arg1	concentrations					825:838	The SF HA concentrations	815:838	The SF HA concentrations	815:838	The SF HA concentrations and size distribution were determined with a sandwich-type enzyme-linked sorbent assay and size-exclusion chromatography.
35934389	9	0	theme	other	1620:1624	arg1	electrodes					1644:1653	other sequential enzyme electrodes	1620:1653	other sequential enzyme electrodes for potential applications	1620:1680	This work provides new ideas for the development of other sequential enzyme electrodes for potential applications.
35934389	7	1	theme	negative	1274:1281	arg1	potential					1283:1291	negative potential	1274:1291	negative potential	1274:1291	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	7	2	dep	starch	1400:1405	arg1	50-3500 mg/L					1422:1433	50-3500 mg/L	1422:1433	50-3500 mg/L	1422:1433	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	7	2	dep	starch	1400:1405	arg1	range					1415:1419	linear range	1408:1419	linear range	1408:1419	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	1	3	theme	single-enzyme-based	286:304	arg1	biosensors					306:315	single-enzyme-based biosensors	286:315	single-enzyme-based biosensors	286:315	The sequential enzyme biosensors hold significant importance in measuring species which are usually hard to process with single-enzyme-based biosensors.
35934389	7	4	theme	reduction	1251:1259	arg1	detection					1261:1269	reduction detection	1251:1269	reduction detection at negative potential without interference	1251:1312	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	8	5	theme	yeast-GOx/RGO/GCE	1462:1478	arg1	sensor					1488:1493	yeast-GOx/RGO/GCE glucose sensor	1462:1493	yeast-GOx/RGO/GCE glucose sensor	1462:1493	Coupled with yeast-GOx/RGO/GCE glucose sensor, both glucose and starch in real samples can be detected satisfactorily.
35934389	8	6	theme	real	1523:1526	arg1	samples					1528:1534	real samples	1523:1534	real samples	1523:1534	Coupled with yeast-GOx/RGO/GCE glucose sensor, both glucose and starch in real samples can be detected satisfactorily.
35934389	4	7	theme	electrode	933:941	arg1	GOx					959:961	the electrode and recombinant GOx	929:961	the electrode and recombinant GOx	929:961	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	7	8	from	potential	1283:1291	arg1	detection					1261:1269	reduction detection	1251:1269	reduction detection at negative potential without interference	1251:1312	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	5	9	theme	sensors	1016:1022	arg1	responses					1003:1011	the current responses	991:1011	the current responses of sensors to starch and glucose	991:1044	Interestingly, the current responses of sensors to starch and glucose are dependent on the displayed enzyme composition, of which the yeast-GA&GOx (2:1) exhibited the highest current.
35934389	5	9	theme	sensors	1016:1022	arg1	dependent					1050:1058	dependent	1050:1058	dependent	1050:1058	Interestingly, the current responses of sensors to starch and glucose are dependent on the displayed enzyme composition, of which the yeast-GA&GOx (2:1) exhibited the highest current.
35934389	4	10	theme	graphene	827:834	arg1	RGO					843:845	RGO	843:845	RGO	843:845	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	10	theme	graphene	827:834	arg1	oxide					836:840	reduced graphene oxide	819:840	reduced graphene oxide (RGO)	819:846	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	6	11	theme	yeast-GA&GOx	1200:1211	arg1	/RGO/GCE					1218:1225	yeast-GA&GOx (2:1)/RGO/GCE	1200:1225	yeast-GA&GOx (2:1)/RGO/GCE	1200:1225	Thereafter, sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE was developed.
35934389	2	12	theme	enzyme	338:343	arg1	electrodes					345:354	sequential enzyme electrodes	327:354	sequential enzyme electrodes	327:354	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	3	13	theme	specific	602:609	arg1	interaction					637:647	the specific cohesion-dockerin protein interaction	598:647	the specific cohesion-dockerin protein interaction	598:647	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	6	14	theme	sequential	1172:1181	arg1	sensor					1190:1195	sequential enzyme sensor	1172:1195	sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE	1172:1225	Thereafter, sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE was developed.
35934389	7	15	theme	linear	1408:1413	arg1	50-3500 mg/L					1422:1433	50-3500 mg/L	1422:1433	50-3500 mg/L	1422:1433	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	7	15	theme	linear	1408:1413	arg1	range					1415:1419	linear range	1408:1419	linear range	1408:1419	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	2	16	theme	sequential	327:336	arg1	electrodes					345:354	sequential enzyme electrodes	327:354	sequential enzyme electrodes	327:354	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	1	17	theme	significant	203:213	arg1	importance					215:224	hold significant importance	198:224	hold significant importance	198:224	The sequential enzyme biosensors hold significant importance in measuring species which are usually hard to process with single-enzyme-based biosensors.
35934389	5	18	theme	highest	1143:1149	arg1	current					1151:1157	the highest current	1139:1157	the highest current	1139:1157	Interestingly, the current responses of sensors to starch and glucose are dependent on the displayed enzyme composition, of which the yeast-GA&GOx (2:1) exhibited the highest current.
35934389	7	19	theme	linear	1369:1374	arg1	range					1376:1380	linear range	1369:1380	linear range: 2.0-100 mg/L	1369:1394	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	0	20	theme	electrochemical	10:24	arg1	biosensor					44:52	Sensitive electrochemical sequential enzyme biosensor	0:52	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose	0:110	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose oxidase-controllably co-displayed yeast recombinant.
35934389	4	21	theme	electron	903:910	arg1	arrived					967:973	arrived	967:973	arrived	967:973	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	21	theme	electron	903:910	arg1	transfer					912:919	the direct electron transfer	892:919	the direct electron transfer between the electrode and recombinant GOx	892:961	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	22	theme	reduced	819:825	arg1	RGO					843:845	RGO	843:845	RGO	843:845	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	22	theme	reduced	819:825	arg1	oxide					836:840	reduced graphene oxide	819:840	reduced graphene oxide (RGO)	819:846	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	0	23	theme	Sensitive	0:8	arg1	biosensor					44:52	Sensitive electrochemical sequential enzyme biosensor	0:52	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose	0:110	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose oxidase-controllably co-displayed yeast recombinant.
35934389	4	24	theme	direct	896:901	arg1	arrived					967:973	arrived	967:973	arrived	967:973	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	24	theme	direct	896:901	arg1	transfer					912:919	the direct electron transfer	892:919	the direct electron transfer between the electrode and recombinant GOx	892:961	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	5	25	theme	current	995:1001	arg1	responses					1003:1011	the current responses	991:1011	the current responses of sensors to starch and glucose	991:1044	Interestingly, the current responses of sensors to starch and glucose are dependent on the displayed enzyme composition, of which the yeast-GA&GOx (2:1) exhibited the highest current.
35934389	5	25	theme	current	995:1001	arg1	dependent					1050:1058	dependent	1050:1058	dependent	1050:1058	Interestingly, the current responses of sensors to starch and glucose are dependent on the displayed enzyme composition, of which the yeast-GA&GOx (2:1) exhibited the highest current.
35934389	3	26	theme	sequential	499:508	arg1	enzymes					510:516	sequential enzymes	499:516	sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios	499:588	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	0	27	theme	enzyme	37:42	arg1	biosensor					44:52	Sensitive electrochemical sequential enzyme biosensor	0:52	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose	0:110	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose oxidase-controllably co-displayed yeast recombinant.
35934389	3	28	theme	cohesion-dockerin	611:627	arg1	interaction					637:647	the specific cohesion-dockerin protein interaction	598:647	the specific cohesion-dockerin protein interaction	598:647	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	7	29	dep	range	1376:1380	arg1	2.0-100 mg/L					1383:1394	2.0-100 mg/L	1383:1394	linear range: 2.0-100 mg/L	1369:1394	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	0	30	theme	sequential	26:35	arg1	biosensor					44:52	Sensitive electrochemical sequential enzyme biosensor	0:52	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose	0:110	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose oxidase-controllably co-displayed yeast recombinant.
35934389	0	31	theme	yeast	146:150	arg1	recombinant					152:162	yeast recombinant	146:162	yeast recombinant	146:162	Sensitive electrochemical sequential enzyme biosensor for glucose and starch based on glucoamylase- and glucose oxidase-controllably co-displayed yeast recombinant.
35934389	6	32	theme	/RGO/GCE	1218:1225	arg1	sensor					1190:1195	sequential enzyme sensor	1172:1195	sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE	1172:1225	Thereafter, sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE was developed.
35934389	3	33	theme	protein	629:635	arg1	interaction					637:647	the specific cohesion-dockerin protein interaction	598:647	the specific cohesion-dockerin protein interaction	598:647	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	1	34	theme	sequential	169:178	arg1	enzyme					180:185	The sequential enzyme	165:185	The sequential enzyme	165:185	The sequential enzyme biosensors hold significant importance in measuring species which are usually hard to process with single-enzyme-based biosensors.
35934389	3	35	theme	yeast	471:475	arg1	surface					477:483	yeast surface	471:483	yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction	471:647	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	9	36	theme	new	1587:1589	arg1	ideas					1591:1595	new ideas	1587:1595	new ideas	1587:1595	This work provides new ideas for the development of other sequential enzyme electrodes for potential applications.
35934389	3	37	theme	glucoamylase	521:532	arg1	enzymes					510:516	sequential enzymes	499:516	sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios	499:588	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	9	38	theme	electrodes	1644:1653	arg1	development					1605:1615	the development	1601:1615	the development of other sequential enzyme electrodes for potential applications	1601:1680	This work provides new ideas for the development of other sequential enzyme electrodes for potential applications.
35934389	5	39	theme	displayed	1067:1075	arg1	composition					1084:1094	the displayed enzyme composition	1063:1094	the displayed enzyme composition	1063:1094	Interestingly, the current responses of sensors to starch and glucose are dependent on the displayed enzyme composition, of which the yeast-GA&GOx (2:1) exhibited the highest current.
35934389	4	40	theme	recombinant	947:957	arg1	GOx					959:961	the electrode and recombinant GOx	929:961	the electrode and recombinant GOx	929:961	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	8	41	theme	glucose	1480:1486	arg1	sensor					1488:1493	yeast-GOx/RGO/GCE glucose sensor	1462:1493	yeast-GOx/RGO/GCE glucose sensor	1462:1493	Coupled with yeast-GOx/RGO/GCE glucose sensor, both glucose and starch in real samples can be detected satisfactorily.
35934389	5	42	theme	enzyme	1077:1082	arg1	composition					1084:1094	the displayed enzyme composition	1063:1094	the displayed enzyme composition	1063:1094	Interestingly, the current responses of sensors to starch and glucose are dependent on the displayed enzyme composition, of which the yeast-GA&GOx (2:1) exhibited the highest current.
35934389	3	43	theme	oxidase	551:557	arg1	enzymes					510:516	sequential enzymes	499:516	sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios	499:588	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	9	44	theme	sequential	1626:1635	arg1	electrodes					1644:1653	other sequential enzyme electrodes	1620:1653	other sequential enzyme electrodes for potential applications	1620:1680	This work provides new ideas for the development of other sequential enzyme electrodes for potential applications.
35934389	2	45	theme	catalytic	395:403	arg1	efficiency					405:414	low catalytic efficiency	391:414	low catalytic efficiency	391:414	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	9	46	theme	potential	1659:1667	arg1	applications					1669:1680	potential applications	1659:1680	potential applications	1659:1680	This work provides new ideas for the development of other sequential enzyme electrodes for potential applications.
35934389	3	47	with	enzymes	510:516	arg1	ratios					583:588	controllable ratios	570:588	controllable ratios	570:588	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	9	48	theme	enzyme	1637:1642	arg1	electrodes					1644:1653	other sequential enzyme electrodes	1620:1653	other sequential enzyme electrodes for potential applications	1620:1680	This work provides new ideas for the development of other sequential enzyme electrodes for potential applications.
35934389	4	49	theme	carbon	858:863	arg1	electrode					865:873	glassy carbon electrode	851:873	glassy carbon electrode (GCE)	851:879	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	49	theme	carbon	858:863	arg1	GCE					876:878	GCE	876:878	GCE	876:878	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	50	theme	modified	741:748	arg1	electrodes					750:759	The modified electrodes	737:759	The modified electrodes	737:759	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	2	51	theme	low	391:393	arg1	efficiency					405:414	low catalytic efficiency	391:414	low catalytic efficiency	391:414	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	2	52	theme	critical	367:374	arg1	insensitivity					417:429	insensitivity	417:429	insensitivity	417:429	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	2	52	theme	critical	367:374	arg1	reproducibility					440:454	poor reproducibility	435:454	poor reproducibility	435:454	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	2	52	theme	critical	367:374	arg1	efficiency					405:414	low catalytic efficiency	391:414	low catalytic efficiency	391:414	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	2	52	theme	critical	367:374	arg1	issues					376:381	critical issues	367:381	critical issues such as low catalytic efficiency, insensitivity and poor reproducibility	367:454	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	3	53	theme	rate-limiting	717:729	arg1	step					731:734	the rate-limiting step	713:734	the rate-limiting step	713:734	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	3	53	theme	rate-limiting	717:729	arg1	starch					672:677	starch	672:677	starch hydrolyzing by GA into glucose	672:708	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	4	54	theme	glassy	851:856	arg1	electrode					865:873	glassy carbon electrode	851:873	glassy carbon electrode (GCE)	851:879	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	4	54	theme	glassy	851:856	arg1	GCE					876:878	GCE	876:878	GCE	876:878	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	3	55	theme	glucose	543:549	arg1	oxidase					551:557	glucose oxidase	543:557	glucose oxidase (GOx)	543:563	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	3	55	theme	glucose	543:549	arg1	GOx					560:562	GOx	560:562	GOx	560:562	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	4	56	theme	whole-cell	804:813	arg1	yeast-GA&GOx					791:802	yeast-GA&GOx whole-cell	791:813	yeast-GA&GOx whole-cell	791:813	The modified electrodes were prepared by immobilizing yeast-GA&GOx whole-cell and reduced graphene oxide (RGO) on glassy carbon electrode (GCE), for which the direct electron transfer between the electrode and recombinant GOx was arrived.
35934389	2	57	theme	poor	435:438	arg1	reproducibility					440:454	poor reproducibility	435:454	poor reproducibility	435:454	However, sequential enzyme electrodes experience critical issues such as low catalytic efficiency, insensitivity and poor reproducibility.
35934389	6	58	theme	2:1	1214:1216	arg1	/RGO/GCE					1218:1225	yeast-GA&GOx (2:1)/RGO/GCE	1200:1225	yeast-GA&GOx (2:1)/RGO/GCE	1200:1225	Thereafter, sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE was developed.
35934389	7	59	dep	glucose	1360:1366	arg1	range					1376:1380	linear range	1369:1380	linear range: 2.0-100 mg/L	1369:1394	Based on reduction detection at negative potential without interference, the sensor is stable and capable of assaying glucose (linear range: 2.0-100 mg/L) or starch (linear range, 50-3500 mg/L), separately.
35934389	6	60	theme	enzyme	1183:1188	arg1	sensor					1190:1195	sequential enzyme sensor	1172:1195	sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE	1172:1225	Thereafter, sequential enzyme sensor of yeast-GA&GOx (2:1)/RGO/GCE was developed.
35934389	8	61	from	starch	1513:1518	arg1	samples					1528:1534	real samples	1523:1534	real samples	1523:1534	Coupled with yeast-GOx/RGO/GCE glucose sensor, both glucose and starch in real samples can be detected satisfactorily.
35934389	3	62	theme	controllable	570:581	arg1	ratios					583:588	controllable ratios	570:588	controllable ratios	570:588	In this work, yeast surface co-displaying sequential enzymes of glucoamylase (GA) and glucose oxidase (GOx) with controllable ratios through the specific cohesion-dockerin protein interaction was explored, by which starch hydrolyzing by GA into glucose is the rate-limiting step.
35934389	8	63	from	glucose	1501:1507	arg1	samples					1528:1534	real samples	1523:1534	real samples	1523:1534	Coupled with yeast-GOx/RGO/GCE glucose sensor, both glucose and starch in real samples can be detected satisfactorily.
36674104	2	0	theme	group	466:470	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	0	theme	group	466:470	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	5	1	theme	week	1231:1234	arg1	groats					1246:1251	a week buckwheat groats	1229:1251	a week buckwheat groats	1229:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	4	2	theme	group	677:681	arg1	A					683:683	group A	677:683	group A	677:683	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	3	theme	standard	688:695	arg1	diet					708:711	a standard low-energy diet	686:711	a standard low-energy diet	686:711	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	4	theme	consumption	831:841	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	4	theme	consumption	831:841	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	4	theme	consumption	831:841	arg1	frequency					818:826	frequency	818:826	frequency	818:826	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	4	theme	consumption	831:841	arg1	amount					807:812	the amount	803:812	the amount	803:812	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	5	5	theme	fermented	1199:1207	arg1	cucumbers					1209:1217	fermented cucumbers	1199:1217	a week fermented cucumbers	1192:1217	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	3	6	used	used	646:649	arg2	types					599:603	two types	595:603	two types of weight-reduction diets (A and B)	595:639	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	0	7	contain	Containing	18:27	arg1	Reduction					112:120	the Reduction	108:120	the Reduction of Body Fat	108:132	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	0	7	contain	Containing	18:27	arg2	Diet					13:16	a Diet	11:16	a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat	11:132	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	4	8	theme	fermented	890:898	arg1	drinks					905:910	fermented milk drinks	890:910	fermented milk drinks (300-400 g)	890:922	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	8	theme	fermented	890:898	arg1	g					921:921	300-400 g	913:921	300-400 g	913:921	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	3	9	theme	multi-center	540:551	arg1	study					588:592	a multi-center, two-arm, parallel, non-randomized study	538:592	a multi-center, two-arm, parallel, non-randomized study	538:592	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	1	10	from	proportions	246:256	arg1	Region					278:283	European Region	269:283	the WHO European Region	261:283	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	2	11	theme	average	474:480	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	11	theme	average	474:480	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	4	12	theme	drinks	905:910	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	12	theme	drinks	905:910	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	2	13	theme	BMI	431:433	arg1	kg/m2					447:451	A average BMI 34.9 ± 4.86 kg/m2	421:451	A average BMI 34.9 ± 4.86 kg/m2	421:451	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	1	14	theme	World	161:165	arg1	Organization					174:185	the World Health Organization (WHO)	157:191	the World Health Organization (WHO)	157:191	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	6	15	theme	body	1315:1318	arg1	mass					1324:1327	body fat mass	1315:1327	body fat mass	1315:1327	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	4	16	theme	intake	880:885	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	16	theme	intake	880:885	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	16	theme	intake	880:885	arg1	frequency					818:826	frequency	818:826	frequency	818:826	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	16	theme	intake	880:885	arg1	amount					807:812	the amount	803:812	the amount	803:812	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	10	17	theme	muciniphila	2106:2116	arg1	bacteria					2118:2125	Akkermansia muciniphila bacteria	2094:2125	Akkermansia muciniphila bacteria	2094:2125	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	9	18	theme	introduced	1809:1818	arg1	intervention					1832:1843	the introduced nutritional intervention	1805:1843	the introduced nutritional intervention	1805:1843	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	10	19	theme	dietary	2038:2044	arg1	changes					2046:2052	dietary changes	2038:2052	dietary changes	2038:2052	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	4	20	theme	fermented	925:933	arg1	cucumbers					935:943	fermented cucumbers	925:943	fermented cucumbers (100 g)	925:951	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	20	theme	fermented	925:933	arg1	g					950:950	100 g	946:950	100 g	946:950	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	9	21	theme	intervention	1832:1843	arg1	influence					1792:1800	the influence	1788:1800	the influence of the introduced nutritional intervention	1788:1843	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	0	22	theme	Body	125:128	arg1	Fat					130:132	Body Fat	125:132	Body Fat	125:132	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	2	23	theme	±	440:440	arg1	kg/m2					447:451	A average BMI 34.9 ± 4.86 kg/m2	421:451	A average BMI 34.9 ± 4.86 kg/m2	421:451	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	7	24	theme	Body	1402:1405	arg1	weight					1407:1412	Body weight	1402:1412	Body weight	1402:1412	Body weight and body composition were measured using the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method.
36674104	11	25	theme	prausnitzii	2287:2297	arg1	bacteria					2299:2306	Faecalibacterium prausnitzii bacteria	2270:2306	Faecalibacterium prausnitzii bacteria	2270:2306	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	8	26	theme	stool	1550:1554	arg1	samples					1556:1562	The stool samples	1546:1562	The stool samples	1546:1562	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	10	27	theme	weight	2009:2014	arg1	reduction					2016:2024	weight reduction	2009:2024	weight reduction	2009:2024	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	11	28	theme	low-energy	2153:2162	arg1	diet					2164:2167	The low-energy diet	2149:2167	The low-energy diet containing sources of natural prebiotics and probiotics	2149:2223	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	3	29	theme	weight-reduction	608:623	arg1	B					638:638	B	638:638	B	638:638	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	3	29	theme	weight-reduction	608:623	arg1	A					632:632	A	632:632	A	632:632	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	3	29	theme	weight-reduction	608:623	arg1	diets					625:629	weight-reduction diets	608:629	weight-reduction diets (A and B)	608:639	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	2	30	theme	women	382:386	arg1	group					370:374	a group	368:374	a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	368:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	30	theme	women	382:386	arg1	women					382:386	56 women	379:386	56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	379:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	31	theme	women	409:413	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	31	theme	women	409:413	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	4	32	theme	water	962:966	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	32	theme	water	962:966	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	8	33	theme	microbiology	1585:1596	arg1	laboratory					1598:1607	the microbiology laboratory	1581:1607	the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed	1581:1779	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	4	34	theme	caloric	749:755	arg1	intake					757:762	individually adjusted caloric intake	727:762	individually adjusted caloric intake of 1100-1300 kcal	727:780	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	6	35	theme	water	1330:1334	arg1	content					1336:1342	water content	1330:1342	water content	1330:1342	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	5	36	theme	products	1135:1142	arg1	intake					1110:1115	daily intake	1104:1115	daily intake of fermented milk products (150 g), highly mineralized water (0.5 L)	1104:1184	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	2	37	theme	±	491:491	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	37	theme	±	491:491	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	9	38	theme	body	1900:1903	arg1	fat					1905:1907	body fat	1900:1907	body fat	1900:1907	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	0	39	theme	Gut	90:92	arg1	Microbiota					94:103	the Gut Microbiota	86:103	the Gut Microbiota	86:103	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	8	40	theme	prausnitzii	1690:1700	arg1	species					1702:1708	Faecalibacterium prausnitzii species	1673:1708	Faecalibacterium prausnitzii species	1673:1708	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	11	41	theme	favorable	2236:2244	arg1	effect					2246:2251	a more favorable effect	2229:2251	a more favorable effect on the number of Faecalibacterium prausnitzii bacteria	2229:2306	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	5	42	theme	standard	1014:1021	arg1	diet					1034:1037	a standard low-energy diet	1012:1037	a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats	1012:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	7	43	theme	TANITA	1480:1485	arg1	analyzer					1494:1501	the Tanita MC-780 MA and TANITA BC-601 analyzer	1455:1501	the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method	1455:1543	Body weight and body composition were measured using the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method.
36674104	7	44	theme	bioelectric	1513:1523	arg1	method					1538:1543	the bioelectric bioimpedance method	1509:1543	the bioelectric bioimpedance method	1509:1543	Body weight and body composition were measured using the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method.
36674104	5	45	theme	fermented	1120:1128	arg1	products					1135:1142	fermented milk products	1120:1142	fermented milk products (150 g)	1120:1150	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	5	45	theme	fermented	1120:1128	arg1	g					1149:1149	150 g	1145:1149	150 g	1145:1149	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	5	45	theme	fermented	1120:1128	arg1	water					1172:1176	highly mineralized water	1153:1176	highly mineralized water (0.5 L)	1153:1184	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	2	46	theme	Mass	355:358	arg1	Index					360:364	Body Mass Index	350:364	Body Mass Index	350:364	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	46	theme	Mass	355:358	arg1	BMI					345:347	BMI	345:347	BMI (Body Mass Index)	345:365	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	8	47	theme	spp.	1649:1652	arg1	quantification					1615:1628	quantification	1615:1628	quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC)	1615:1765	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	1	48	theme	epidemic	237:244	arg1	proportions					246:256	epidemic proportions	237:256	epidemic proportions	237:256	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	8	49	theme	muciniphila	1723:1733	arg1	quantification					1615:1628	quantification	1615:1628	quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC)	1615:1765	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	6	50	theme	hip	1383:1385	arg1	circumference					1387:1399	hip circumference	1383:1399	hip circumference	1383:1399	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	4	51	theme	oil	988:990	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	51	theme	oil	988:990	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	5	52	theme	group	1003:1007	arg1	B					1009:1009	group B	1003:1009	group B	1003:1009	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	3	53	dep	multi-center	540:551	arg1	non-randomized					573:586	non-randomized	573:586	non-randomized	573:586	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	3	53	dep	multi-center	540:551	arg1	parallel					563:570	parallel	563:570	parallel	563:570	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	3	53	dep	multi-center	540:551	arg1	two-arm					554:560	two-arm	554:560	two-arm	554:560	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	7	54	theme	BC-601	1487:1492	arg1	analyzer					1494:1501	the Tanita MC-780 MA and TANITA BC-601 analyzer	1455:1501	the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method	1455:1543	Body weight and body composition were measured using the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method.
36674104	9	55	theme	significant	1862:1872	arg1	reduction					1874:1882	a statistically significant reduction	1846:1882	a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference	1846:1951	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	8	56	theme	total	1739:1743	arg1	TBC					1762:1764	TBC	1762:1764	TBC	1762:1764	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	8	56	theme	total	1739:1743	arg1	count					1755:1759	total bacterial count	1739:1759	total bacterial count (TBC)	1739:1765	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	4	57	from	increase	791:798	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	57	from	increase	791:798	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	57	from	increase	791:798	arg1	frequency					818:826	frequency	818:826	frequency	818:826	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	57	from	increase	791:798	arg1	amount					807:812	the amount	803:812	the amount	803:812	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	5	58	theme	caloric	1066:1072	arg1	intake					1074:1079	individually adjusted caloric intake	1044:1079	individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats	1044:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	8	59	theme	count	1755:1759	arg1	quantification					1615:1628	quantification	1615:1628	quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC)	1615:1765	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	6	60	theme	waist	1358:1362	arg1	circumference					1364:1376	waist circumference	1358:1376	waist circumference	1358:1376	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	2	61	dep	aged	388:391	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	61	dep	aged	388:391	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	7	62	theme	body	1418:1421	arg1	composition					1423:1433	body composition	1418:1433	body composition	1418:1433	Body weight and body composition were measured using the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method.
36674104	11	63	contain	containing	2169:2178	arg2	sources					2180:2186	sources	2180:2186	sources of natural prebiotics and probiotics	2180:2223	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	11	63	contain	containing	2169:2178	arg1	diet					2164:2167	The low-energy diet	2149:2167	The low-energy diet containing sources of natural prebiotics and probiotics	2149:2223	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	11	64	theme	prebiotics	2199:2208	arg1	sources					2180:2186	sources	2180:2186	sources of natural prebiotics and probiotics	2180:2223	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	4	65	theme	cod	978:980	arg1	oil					988:990	cod liver oil	978:990	cod liver oil (5 mL)	978:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	65	theme	cod	978:980	arg1	mL					995:996	5 mL	993:996	5 mL	993:996	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	8	66	theme	Faecalibacterium	1673:1688	arg1	species					1702:1708	Faecalibacterium prausnitzii species	1673:1708	Faecalibacterium prausnitzii species	1673:1708	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	11	67	theme	Faecalibacterium	2270:2285	arg1	bacteria					2299:2306	Faecalibacterium prausnitzii bacteria	2270:2306	Faecalibacterium prausnitzii bacteria	2270:2306	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	4	68	theme	kcal	777:780	arg1	intake					757:762	individually adjusted caloric intake	727:762	individually adjusted caloric intake of 1100-1300 kcal	727:780	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	5	69	with	intake	1074:1079	arg1	intake					1110:1115	daily intake	1104:1115	daily intake of fermented milk products (150 g), highly mineralized water (0.5 L)	1104:1184	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	2	70	theme	women	460:464	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	70	theme	women	460:464	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	4	71	theme	milk	900:903	arg1	drinks					905:910	fermented milk drinks	890:910	fermented milk drinks (300-400 g)	890:922	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	71	theme	milk	900:903	arg1	g					921:921	300-400 g	913:921	300-400 g	913:921	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	11	72	theme	probiotics	2214:2223	arg1	sources					2180:2186	sources	2180:2186	sources of natural prebiotics and probiotics	2180:2223	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	5	73	theme	milk	1130:1133	arg1	products					1135:1142	fermented milk products	1120:1142	fermented milk products (150 g)	1120:1150	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	5	73	theme	milk	1130:1133	arg1	g					1149:1149	150 g	1145:1149	150 g	1145:1149	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	5	73	theme	milk	1130:1133	arg1	water					1172:1176	highly mineralized water	1153:1176	highly mineralized water (0.5 L)	1153:1184	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	2	74	theme	BMI	482:484	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	74	theme	BMI	482:484	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	5	75	theme	kcal	1094:1097	arg1	intake					1074:1079	individually adjusted caloric intake	1044:1079	individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats	1044:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	9	76	from	reduction	1874:1882	arg1	circumference					1939:1951	hip circumference	1935:1951	hip circumference	1935:1951	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	9	76	from	reduction	1874:1882	arg1	weight					1892:1897	body weight	1887:1897	body weight	1887:1897	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	9	76	from	reduction	1874:1882	arg1	fat					1905:1907	body fat	1900:1907	body fat	1900:1907	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	9	76	from	reduction	1874:1882	arg1	circumference					1916:1928	waist circumference	1910:1928	waist circumference	1910:1928	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	2	77	theme	B	472:472	arg1	kg/m2					498:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2	406:502	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	77	theme	B	472:472	arg1	years					399:403	25-45 years	393:403	25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	393:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	4	78	theme	daily	874:878	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	5	79	theme	daily	1104:1108	arg1	intake					1110:1115	daily intake	1104:1115	daily intake of fermented milk products (150 g), highly mineralized water (0.5 L)	1104:1184	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	4	80	theme	low-energy	697:706	arg1	diet					708:711	a standard low-energy diet	686:711	a standard low-energy diet	686:711	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	6	81	theme	fat	1320:1322	arg1	mass					1324:1327	body fat mass	1315:1327	body fat mass	1315:1327	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	10	82	theme	bacteria	2118:2125	arg1	number					2084:2089	the number	2080:2089	the number of Akkermansia muciniphila bacteria	2080:2125	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	5	83	theme	buckwheat	1236:1244	arg1	groats					1246:1251	a week buckwheat groats	1229:1251	a week buckwheat groats	1229:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	3	84	dep	diets	625:629	arg1	B					638:638	B	638:638	B	638:638	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	3	84	dep	diets	625:629	arg1	A					632:632	A	632:632	A	632:632	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	3	84	dep	diets	625:629	arg1	diets					625:629	weight-reduction diets	608:629	weight-reduction diets (A and B)	608:639	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	1	85	theme	Health	167:172	arg1	Organization					174:185	the World Health Organization (WHO)	157:191	the World Health Organization (WHO)	157:191	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	4	86	theme	cucumbers	935:943	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	86	theme	cucumbers	935:943	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	10	87	theme	Akkermansia	2094:2104	arg1	bacteria					2118:2125	Akkermansia muciniphila bacteria	2094:2125	Akkermansia muciniphila bacteria	2094:2125	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	9	88	theme	nutritional	1820:1830	arg1	intervention					1832:1843	the introduced nutritional intervention	1805:1843	the introduced nutritional intervention	1805:1843	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	0	89	theme	Fat	130:132	arg1	Reduction					112:120	the Reduction	108:120	the Reduction of Body Fat	108:132	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	5	90	used	used	1257:1260	arg2	diet					1034:1037	a standard low-energy diet	1012:1037	a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats	1012:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	6	91	theme	body	1302:1305	arg1	weight					1307:1312	body weight	1302:1312	body weight	1302:1312	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	3	92	theme	3-month	658:664	arg1	period					666:671	a 3-month period	656:671	a 3-month period	656:671	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	11	93	theme	bacteria	2299:2306	arg1	number					2260:2265	the number	2256:2265	the number of Faecalibacterium prausnitzii bacteria	2256:2306	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	0	94	theme	Probiotics	55:64	arg1	Sources					29:35	Sources	29:35	Sources of Prebiotics and Probiotics	29:64	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	0	94	theme	Probiotics	55:64	arg1	Modification					70:81	Modification	70:81	Modification of the Gut Microbiota	70:103	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	2	95	theme	aged	388:391	arg1	women					382:386	56 women	379:386	56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2)	379:503	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	3	96	theme	diets	625:629	arg1	types					599:603	two types	595:603	two types of weight-reduction diets (A and B)	595:639	In a multi-center, two-arm, parallel, non-randomized study, two types of weight-reduction diets (A and B) were used over a 3-month period.
36674104	10	97	theme	reduction	2016:2024	arg1	influence					1996:2004	the influence	1992:2004	the influence of weight reduction, as well as dietary changes	1992:2052	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	2	98	theme	average	423:429	arg1	kg/m2					447:451	A average BMI 34.9 ± 4.86 kg/m2	421:451	A average BMI 34.9 ± 4.86 kg/m2	421:451	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	6	99	theme	body	1345:1348	arg1	height					1350:1355	body height	1345:1355	body height	1345:1355	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	11	100	theme	standard	2324:2331	arg1	diet					2333:2336	the standard diet	2320:2336	the standard diet	2320:2336	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	9	101	theme	body	1887:1890	arg1	weight					1892:1897	body weight	1887:1897	body weight	1887:1897	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	6	102	theme	following	1267:1275	arg1	measurements					1277:1288	The following measurements	1263:1288	The following measurements	1263:1288	The following measurements were taken: body weight, body fat mass, water content, body height, waist circumference, and hip circumference.
36674104	5	103	theme	mineralized	1160:1170	arg1	water					1172:1176	highly mineralized water	1153:1176	highly mineralized water (0.5 L)	1153:1184	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	5	103	theme	mineralized	1160:1170	arg1	L					1183:1183	0.5 L	1179:1183	0.5 L	1179:1183	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	5	103	theme	mineralized	1160:1170	arg1	products					1135:1142	fermented milk products	1120:1142	fermented milk products (150 g)	1120:1150	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	0	104	theme	Microbiota	94:103	arg1	Sources					29:35	Sources	29:35	Sources of Prebiotics and Probiotics	29:64	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	0	104	theme	Microbiota	94:103	arg1	Modification					70:81	Modification	70:81	Modification of the Gut Microbiota	70:103	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	7	105	theme	bioimpedance	1525:1536	arg1	method					1538:1543	the bioelectric bioimpedance method	1509:1543	the bioelectric bioimpedance method	1509:1543	Body weight and body composition were measured using the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method.
36674104	8	106	theme	species	1702:1708	arg1	quantification					1615:1628	quantification	1615:1628	quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC)	1615:1765	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	4	107	theme	adjusted	740:747	arg1	intake					757:762	individually adjusted caloric intake	727:762	individually adjusted caloric intake of 1100-1300 kcal	727:780	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	10	108	from	increase	2068:2075	arg1	number					2084:2089	the number	2080:2089	the number of Akkermansia muciniphila bacteria	2080:2125	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	10	108	from	increase	2068:2075	arg1	women					2134:2138	the women	2130:2138	the women studied	2130:2146	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	1	109	theme	adults	310:315	arg1	%					305:305	almost 60%	296:305	almost 60% of adults	296:315	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	1	109	theme	adults	310:315	arg1	adults					310:315	adults	310:315	adults	310:315	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	11	110	from	effect	2246:2251	arg1	number					2260:2265	the number	2256:2265	the number of Faecalibacterium prausnitzii bacteria	2256:2306	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	10	111	theme	changes	2046:2052	arg1	influence					1996:2004	the influence	1992:2004	the influence of weight reduction, as well as dietary changes	1992:2052	Under the influence of weight reduction, as well as dietary changes, there was an increase in the number of Akkermansia muciniphila bacteria in the women studied.
36674104	4	112	theme	mineral	954:960	arg1	water					962:966	mineral water	954:966	mineral water (1 L)	954:972	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	112	theme	mineral	954:960	arg1	L					971:971	1 L	969:971	1 L	969:971	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	9	113	theme	waist	1910:1914	arg1	circumference					1916:1928	waist circumference	1910:1928	waist circumference	1910:1928	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	0	114	theme	Diet	13:16	arg1	Effects					0:6	Effects	0:6	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.	0:133	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	11	115	contain	had	2225:2227	arg1	diet					2164:2167	The low-energy diet	2149:2167	The low-energy diet containing sources of natural prebiotics and probiotics	2149:2223	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	11	115	contain	had	2225:2227	arg2	effect					2246:2251	a more favorable effect	2229:2251	a more favorable effect on the number of Faecalibacterium prausnitzii bacteria	2229:2306	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	2	116	theme	Body	350:353	arg1	Index					360:364	Body Mass Index	350:364	Body Mass Index	350:364	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	2	116	theme	Body	350:353	arg1	BMI					345:347	BMI	345:347	BMI (Body Mass Index)	345:365	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	1	117	dep	Region	278:283	arg1	WHO					265:267	WHO	265:267	WHO	265:267	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	2	118	theme	BMI	345:347	arg1	assessment					331:340	the assessment	327:340	the assessment of BMI (Body Mass Index)	327:365	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	8	119	theme	spp.	1667:1670	arg1	quantification					1615:1628	quantification	1615:1628	quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC)	1615:1765	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	2	120	dep	women	409:413	arg1	group					415:419	group	415:419	group	415:419	Based on the assessment of BMI (Body Mass Index), a group of 56 women aged 25-45 years (31 women group A average BMI 34.9 ± 4.86 kg/m2 and 25 women group B average BMI 33.4 ± 4.02 kg/m2) were qualified for the study.
36674104	8	121	theme	bacterial	1745:1753	arg1	TBC					1762:1764	TBC	1762:1764	TBC	1762:1764	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	8	121	theme	bacterial	1745:1753	arg1	count					1755:1759	total bacterial count	1739:1759	total bacterial count (TBC)	1739:1765	The stool samples were analyzed in the microbiology laboratory where quantification of Bifidobcaterium spp., Bacteroides spp., Faecalibacterium prausnitzii species, Akkermansia muciniphila and total bacterial count (TBC) was performed.
36674104	5	122	theme	adjusted	1057:1064	arg1	intake					1074:1079	individually adjusted caloric intake	1044:1079	individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats	1044:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	9	123	theme	hip	1935:1937	arg1	circumference					1939:1951	hip circumference	1935:1951	hip circumference	1935:1951	Under the influence of the introduced nutritional intervention, a statistically significant reduction in body weight, body fat, waist circumference, and hip circumference was demonstrated after 3 months.
36674104	0	124	theme	Prebiotics	40:49	arg1	Sources					29:35	Sources	29:35	Sources of Prebiotics and Probiotics	29:64	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	0	124	theme	Prebiotics	40:49	arg1	Modification					70:81	Modification	70:81	Modification of the Gut Microbiota	70:103	Effects of a Diet Containing Sources of Prebiotics and Probiotics and Modification of the Gut Microbiota on the Reduction of Body Fat.
36674104	7	125	theme	MA	1473:1474	arg1	analyzer					1494:1501	the Tanita MC-780 MA and TANITA BC-601 analyzer	1455:1501	the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method	1455:1543	Body weight and body composition were measured using the Tanita MC-780 MA and TANITA BC-601 analyzer using the bioelectric bioimpedance method.
36674104	1	126	theme	European	269:276	arg1	Region					278:283	European Region	269:283	the WHO European Region	261:283	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	5	127	dep	week	1194:1197	arg1	cucumbers					1209:1217	fermented cucumbers	1199:1217	a week fermented cucumbers	1192:1217	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	4	128	theme	sauerkraut	846:855	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	128	theme	sauerkraut	846:855	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	11	129	theme	natural	2191:2197	arg1	prebiotics					2199:2208	natural prebiotics	2191:2208	natural prebiotics	2191:2208	The low-energy diet containing sources of natural prebiotics and probiotics had a more favorable effect on the number of Faecalibacterium prausnitzii bacteria compared to the standard diet.
36674104	4	130	theme	liver	982:986	arg1	oil					988:990	cod liver oil	978:990	cod liver oil (5 mL)	978:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	130	theme	liver	982:986	arg1	mL					995:996	5 mL	993:996	5 mL	993:996	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	1	131	dep	Organization	174:185	arg1	report					193:198	report	193:198	report	193:198	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	1	131	dep	Organization	174:185	arg1	WHO					188:190	WHO	188:190	WHO	188:190	In 2022, according to the World Health Organization (WHO) report, overweight and obesity have reached epidemic proportions in the WHO European Region, affecting almost 60% of adults.
36674104	5	132	theme	low-energy	1023:1032	arg1	diet					1034:1037	a standard low-energy diet	1012:1037	a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats	1012:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
36674104	4	133	used	used	717:720	arg2	diet					708:711	a standard low-energy diet	686:711	a standard low-energy diet	686:711	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	134	theme	groats	861:866	arg1	consumption					831:841	consumption	831:841	consumption of sauerkraut and groats	831:866	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	4	134	theme	groats	861:866	arg1	intake					880:885	a daily intake	872:885	a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL)	872:997	In group A, a standard low-energy diet was used with individually adjusted caloric intake of 1100-1300 kcal, with an increase in the amount and frequency of consumption of sauerkraut and groats and a daily intake of fermented milk drinks (300-400 g), fermented cucumbers (100 g), mineral water (1 L) and cod liver oil (5 mL).
36674104	5	135	with	diet	1034:1037	arg1	intake					1074:1079	individually adjusted caloric intake	1044:1079	individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats	1044:1251	In group B, a standard low-energy diet with individually adjusted caloric intake of 1100-1300 kcal with daily intake of fermented milk products (150 g), highly mineralized water (0.5 L), once a week fermented cucumbers, and once a week buckwheat groats was used.
35337551	1	0	with	diets	241:245	arg1	sources					269:275	different starch sources	252:275	different starch sources	252:275	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	4	1	dep	decreased	878:886	arg1	P < 0.05					889:896	P < 0.05	889:896	P < 0.05	889:896	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	5	2	theme	microbial	1050:1058	arg1	functions					1060:1068	microbial functions	1050:1068	microbial functions	1050:1068	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	7	3	theme	related	1537:1543	arg1	metabolites					1525:1535	the resultant metabolites	1511:1535	the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol)	1511:1772	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	7	4	theme	amino	1548:1552	arg1	acids					1554:1558	amino acids	1548:1558	amino acids	1548:1558	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	6	5	theme	pathway	1295:1301	arg1	analysis					1303:1310	KEGG pathway analysis	1290:1310	KEGG pathway analysis	1290:1310	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	6	5	theme	pathway	1295:1301	arg1	Meanwhile					1279:1287	Meanwhile	1279:1287	Meanwhile	1279:1287	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	8	6	from	ratio	1999:2003	arg1	diets					2034:2038	diets	2034:2038	diets	2034:2038	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	7	7	theme	resultant	1515:1523	arg1	metabolites					1525:1535	the resultant metabolites	1511:1535	the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol)	1511:1772	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	7	8	dep	derivatives	1570:1580	arg1	such					1583:1586	such	1583:1586	such	1583:1586	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	3	9	theme	diets	637:641	arg1	amylopectin					614:624	amylopectin	614:624	amylopectin	614:624	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	3	9	theme	diets	637:641	arg1	amylose					602:608	amylose	602:608	amylose	602:608	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	4	10	theme	PS	746:747	arg1	diet					749:752	the PS diet	742:752	the PS diet	742:752	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	8	11	theme	intestinal	2061:2070	arg1	health					2072:2077	intestinal health	2061:2077	intestinal health of pigs	2061:2085	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	4	12	theme	amylolytic	801:810	arg1	bacteria					812:819	amylolytic bacteria	801:819	amylolytic bacteria	801:819	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	3	13	theme	starch	540:545	arg1	diet					552:555	a tapioca starch (TS) diet	530:555	a tapioca starch (TS) diet	530:555	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	4	14	from	Porphyromonas	973:985	arg1	cecum					1010:1014	the cecum	1006:1014	the cecum of pigs	1006:1022	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	7	15	dep	changed	1784:1790	arg1	P < 0.05					1793:1800	P < 0.05	1793:1800	P < 0.05	1793:1800	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	8	16	theme	responsive	1929:1938	arg1	changes					1940:1946	responsive changes	1929:1946	responsive changes to diets with different starch sources	1929:1985	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	0	17	theme	different	136:144	arg1	sources					153:159	different starch sources	136:159	different starch sources	136:159	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	4	18	from	Tyzzerella	961:970	arg1	cecum					1010:1014	the cecum	1006:1014	the cecum of pigs	1006:1022	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	7	19	theme	fatty	1687:1691	arg1	acids					1693:1697	fatty acids	1687:1697	fatty acids	1687:1697	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	7	20	theme	pigs	1819:1822	arg1	cecum					1810:1814	the cecum	1806:1814	the cecum of pigs fed the PS diet	1806:1838	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	6	21	from	trend	1332:1336	arg1	pathways					1468:1475	the lipid metabolism pathways	1447:1475	the lipid metabolism pathways in the PS group	1447:1491	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	6	22	theme	decreasing	1321:1330	arg1	P < 0.1					1339:1345	P < 0.1	1339:1345	P < 0.1	1339:1345	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	6	22	theme	decreasing	1321:1330	arg1	trend					1332:1336	a decreasing trend	1319:1336	a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways	1319:1403	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	8	23	theme	starch	1972:1977	arg1	sources					1979:1985	different starch sources	1962:1985	different starch sources	1962:1985	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	0	24	with	diets	125:129	arg1	sources					153:159	different starch sources	136:159	different starch sources	136:159	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	3	25	theme	starch	566:571	arg1	diet					578:581	a pea starch (PS) diet	560:581	a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively)	560:675	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	4	26	theme	spp.	868:871	arg1	abundance					788:796	the relative abundance	775:796	the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp.	775:871	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	4	27	theme	pigs	1019:1022	arg1	cecum					1010:1014	the cecum	1006:1014	the cecum of pigs	1006:1022	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	5	28	theme	TS	1258:1259	arg1	P < 0.05					1268:1275	P < 0.05	1268:1275	P < 0.05	1268:1275	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	28	theme	TS	1258:1259	arg1	group					1261:1265	the TS group	1254:1265	the TS group (P < 0.05)	1254:1276	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	6	29	theme	acid	1380:1383	arg1	metabolism					1385:1394	amino acid metabolism	1374:1394	amino acid metabolism	1374:1394	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	2	30	theme	pigs	505:508	arg1	pens					495:498	eight replicate pens	479:498	eight replicate pens of 3 pigs each	479:513	In this study, 48 crossbred growing barrows were randomly allocated to 2 treatment groups with eight replicate pens of 3 pigs each.
35337551	6	31	theme	lipid	1451:1455	arg1	pathways					1468:1475	the lipid metabolism pathways	1447:1475	the lipid metabolism pathways in the PS group	1447:1491	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	0	32	theme	pigs	116:119	arg1	function					87:94	function	87:94	function	87:94	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	0	32	theme	pigs	116:119	arg1	metabolites					101:111	metabolites	101:111	metabolites	101:111	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	0	32	theme	pigs	116:119	arg1	composition					74:84	the cecal microbial composition	54:84	the cecal microbial composition	54:84	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	1	33	theme	pigs	378:381	arg1	cecum					359:363	the cecum	355:363	the cecum of finishing pigs	355:381	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	7	34	theme	pyroglutamic	1663:1674	arg1	acid					1676:1679	pyroglutamic acid	1663:1679	pyroglutamic acid	1663:1679	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	0	35	theme	Integrated	0:9	arg1	analysis					37:44	Integrated metagenomics-metabolomics analysis	0:44	Integrated metagenomics-metabolomics analysis	0:44	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	1	36	theme	microbial	284:292	arg1	community					294:302	the microbial community	280:302	the microbial community	280:302	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	6	37	theme	energy	1352:1357	arg1	metabolism					1359:1368	energy metabolism	1352:1368	energy metabolism	1352:1368	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	4	38	theme	relative	903:910	arg1	abundance					912:920	the relative abundance	899:920	the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs	899:1022	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	7	39	theme	increased	1629:1637	arg1	acid					1654:1657	increased γ-aminobutyric acid	1629:1657	increased γ-aminobutyric acid	1629:1657	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	2	40	theme	crossbred	402:410	arg1	barrows					420:426	48 crossbred growing barrows	399:426	48 crossbred growing barrows	399:426	In this study, 48 crossbred growing barrows were randomly allocated to 2 treatment groups with eight replicate pens of 3 pigs each.
35337551	2	41	theme	treatment	457:465	arg1	groups					467:472	2 treatment groups	455:472	2 treatment groups	455:472	In this study, 48 crossbred growing barrows were randomly allocated to 2 treatment groups with eight replicate pens of 3 pigs each.
35337551	4	42	theme	TS	733:734	arg1	diet					736:739	the TS diet	729:739	the TS diet	729:739	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	0	43	theme	microbial	64:72	arg1	composition					74:84	the cecal microbial composition	54:84	the cecal microbial composition	54:84	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	7	44	theme	decreased	1591:1599	arg1	histamine					1601:1609	histamine	1601:1609	histamine	1601:1609	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	8	45	theme	amylose	2008:2014	arg1	ratio					1999:2003	a high ratio	1992:2003	a high ratio of amylose to amylopectin in diets	1992:2038	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	8	45	theme	amylose	2008:2014	arg1	beneficial					2047:2056	beneficial	2047:2056	beneficial	2047:2056	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	5	46	theme	carbohydrate-active	1085:1103	arg1	AA3					1128:1130	AA3	1128:1130	AA3	1128:1130	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	46	theme	carbohydrate-active	1085:1103	arg1	AA6					1137:1139	AA6	1137:1139	AA6	1137:1139	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	46	theme	carbohydrate-active	1085:1103	arg1	GH73					1122:1125	GH73	1122:1125	GH73	1122:1125	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	46	theme	carbohydrate-active	1085:1103	arg1	enzymes					1105:1111	11 carbohydrate-active enzymes	1082:1111	11 carbohydrate-active enzymes	1082:1111	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	4	47	dep	increased	754:762	arg1	P < 0.05					765:772	P < 0.05	765:772	P < 0.05	765:772	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	1	48	theme	starch	262:267	arg1	sources					269:275	different starch sources	252:275	different starch sources	252:275	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	4	49	theme	inflammatory	930:941	arg1	Porphyromonas					973:985	Porphyromonas	973:985	Porphyromonas	973:985	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	4	49	theme	inflammatory	930:941	arg1	Tannerella					992:1001	Tannerella	992:1001	Tannerella	992:1001	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	4	49	theme	inflammatory	930:941	arg1	Tyzzerella					961:970	Tyzzerella	961:970	Tyzzerella	961:970	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	4	49	theme	inflammatory	930:941	arg1	bacteria					943:950	some inflammatory bacteria	925:950	some inflammatory bacteria	925:950	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	1	50	from	functions	315:323	arg1	community					294:302	the microbial community	280:302	the microbial community	280:302	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	5	51	theme	functions	1060:1068	arg1	analysis					1038:1045	analysis	1038:1045	analysis of microbial functions	1038:1068	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	6	52	theme	KEGG	1290:1293	arg1	analysis					1303:1310	KEGG pathway analysis	1290:1310	KEGG pathway analysis	1290:1310	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	6	52	theme	KEGG	1290:1293	arg1	Meanwhile					1279:1287	Meanwhile	1279:1287	Meanwhile	1279:1287	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	7	53	dep	acids	1693:1697	arg1	such					1711:1714	such	1711:1714	such	1711:1714	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	4	54	from	Tannerella	992:1001	arg1	cecum					1010:1014	the cecum	1006:1014	the cecum of pigs	1006:1022	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	7	55	theme	increased	1719:1727	arg1	tetradecanoylcarnitine					1729:1750	increased tetradecanoylcarnitine	1719:1750	increased tetradecanoylcarnitine	1719:1750	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	8	56	theme	cecal	1887:1891	arg1	microbiota					1893:1902	the cecal microbiota	1883:1902	the cecal microbiota	1883:1902	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	3	57	theme	tapioca	532:538	arg1	starch					540:545	tapioca starch	532:545	a tapioca starch (TS) diet	530:555	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	3	57	theme	tapioca	532:538	arg1	TS					548:549	TS	548:549	TS	548:549	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	8	58	theme	pigs	2082:2085	arg1	health					2072:2077	intestinal health	2061:2077	intestinal health of pigs	2061:2085	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	0	59	theme	starch	146:151	arg1	sources					153:159	different starch sources	136:159	different starch sources	136:159	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	4	60	theme	bacteria	812:819	arg1	abundance					788:796	the relative abundance	775:796	the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp.	775:871	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	7	61	theme	PS	1832:1833	arg1	diet					1835:1838	the PS diet	1828:1838	the PS diet	1828:1838	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	1	62	from	effects	230:236	arg1	community					294:302	the microbial community	280:302	the microbial community	280:302	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	6	63	theme	significant	1412:1422	arg1	P < 0.05					1434:1441	P < 0.05	1434:1441	P < 0.05	1434:1441	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	6	63	theme	significant	1412:1422	arg1	increase					1424:1431	a significant increase	1410:1431	a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group	1410:1491	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	4	64	theme	relative	779:786	arg1	abundance					788:796	the relative abundance	775:796	the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp.	775:871	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	3	65	theme	pea	562:564	arg1	starch					566:571	pea starch	562:571	a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively)	560:675	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	3	65	theme	pea	562:564	arg1	PS					574:575	PS	574:575	PS	574:575	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	6	66	from	pathways	1468:1475	arg1	group					1487:1491	the PS group	1480:1491	the PS group	1480:1491	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	6	67	theme	PS	1484:1485	arg1	group					1487:1491	the PS group	1480:1491	the PS group	1480:1491	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	8	68	theme	different	1962:1970	arg1	sources					1979:1985	different starch sources	1962:1985	different starch sources	1962:1985	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	2	69	dep	pens	495:498	arg1	replicate					485:493	replicate	485:493	replicate	485:493	In this study, 48 crossbred growing barrows were randomly allocated to 2 treatment groups with eight replicate pens of 3 pigs each.
35337551	4	70	theme	such	822:825	arg1	bacteria					812:819	amylolytic bacteria	801:819	amylolytic bacteria	801:819	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	6	71	theme	metabolism	1457:1466	arg1	pathways					1468:1475	the lipid metabolism pathways	1447:1475	the lipid metabolism pathways in the PS group	1447:1491	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	1	72	theme	finishing	368:376	arg1	pigs					378:381	finishing pigs	368:381	finishing pigs	368:381	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	6	73	theme	amino	1374:1378	arg1	metabolism					1385:1394	amino acid metabolism	1374:1394	amino acid metabolism	1374:1394	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	1	74	theme	diets	241:245	arg1	metabolites					340:350	resultant metabolites	330:350	resultant metabolites	330:350	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	1	74	theme	diets	241:245	arg1	functions					315:323	metabolic functions	305:323	metabolic functions	305:323	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	1	74	theme	diets	241:245	arg1	effects					230:236	the effects	226:236	the effects of diets with different starch sources on the microbial community	226:302	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	5	75	theme	PS	1163:1164	arg1	P < 0.05					1173:1180	P < 0.05	1173:1180	P < 0.05	1173:1180	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	75	theme	PS	1163:1164	arg1	group					1166:1170	the PS group	1159:1170	the PS group (P < 0.05)	1159:1181	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	0	76	theme	metagenomics-metabolomics	11:35	arg1	analysis					37:44	Integrated metagenomics-metabolomics analysis	0:44	Integrated metagenomics-metabolomics analysis	0:44	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	7	77	theme	γ-aminobutyric	1639:1652	arg1	acid					1654:1657	increased γ-aminobutyric acid	1629:1657	increased γ-aminobutyric acid	1629:1657	Correspondingly, the resultant metabolites related to amino acids and their derivatives (such as decreased histamine and indole, while increased γ-aminobutyric acid and pyroglutamic acid), and fatty acids and lipids (such as increased tetradecanoylcarnitine and monoacylglycerol) were also changed (P < 0.05) in the cecum of pigs fed the PS diet.
35337551	6	78	dep	metabolism	1359:1368	arg1	pathways					1396:1403	pathways	1396:1403	pathways	1396:1403	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	6	79	from	increase	1424:1431	arg1	pathways					1468:1475	the lipid metabolism pathways	1447:1475	the lipid metabolism pathways in the PS group	1447:1491	Meanwhile, KEGG pathway analysis showed a decreasing trend (P < 0.1) for energy metabolism and amino acid metabolism pathways, and a significant increase (P < 0.05) in the lipid metabolism pathways in the PS group.
35337551	2	80	dep	pigs	505:508	arg1	each					510:513	each	510:513	each	510:513	In this study, 48 crossbred growing barrows were randomly allocated to 2 treatment groups with eight replicate pens of 3 pigs each.
35337551	2	81	theme	growing	412:418	arg1	barrows					420:426	48 crossbred growing barrows	399:426	48 crossbred growing barrows	399:426	In this study, 48 crossbred growing barrows were randomly allocated to 2 treatment groups with eight replicate pens of 3 pigs each.
35337551	0	82	theme	cecal	58:62	arg1	composition					74:84	the cecal microbial composition	54:84	the cecal microbial composition	54:84	Integrated metagenomics-metabolomics analysis reveals the cecal microbial composition, function, and metabolites of pigs fed diets with different starch sources.
35337551	1	83	theme	metabolic	305:313	arg1	functions					315:323	metabolic functions	305:323	metabolic functions	305:323	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	1	84	from	metabolites	340:350	arg1	community					294:302	the microbial community	280:302	the microbial community	280:302	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	5	85	theme	other	1193:1197	arg1	GH35					1221:1224	GH35	1221:1224	GH35	1221:1224	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	85	theme	other	1193:1197	arg1	GH53					1231:1234	GH53	1231:1234	GH53	1231:1234	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	85	theme	other	1193:1197	arg1	GH2					1216:1218	GH2	1216:1218	GH2	1216:1218	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	5	85	theme	other	1193:1197	arg1	enzymes					1199:1205	26 other enzymes	1190:1205	26 other enzymes	1190:1205	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	1	86	theme	resultant	330:338	arg1	metabolites					340:350	resultant metabolites	330:350	resultant metabolites	330:350	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35337551	8	87	theme	high	1994:1997	arg1	ratio					1999:2003	a high ratio	1992:2003	a high ratio of amylose to amylopectin in diets	1992:2038	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	8	87	theme	high	1994:1997	arg1	beneficial					2047:2056	beneficial	2047:2056	beneficial	2047:2056	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	4	88	theme	bacteria	943:950	arg1	abundance					912:920	the relative abundance	899:920	the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs	899:1022	The results showed that, compared with the TS diet, the PS diet increased (P < 0.05) the relative abundance of amylolytic bacteria, such as Lactobacillus spp., and Streptococcus spp., and decreased (P < 0.05) the relative abundance of some inflammatory bacteria, such as Tyzzerella, Porphyromonas, and Tannerella in the cecum of pigs.
35337551	8	89	with	diets	1951:1955	arg1	sources					1979:1985	different starch sources	1962:1985	different starch sources	1962:1985	In summary, these findings indicated that the cecal microbiota and metabolism underwent responsive changes to diets with different starch sources, and a high ratio of amylose to amylopectin in diets may be beneficial to intestinal health of pigs.
35337551	3	90	dep	diet	578:581	arg1	0.44					657:660	0.44	657:660	0.44	657:660	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	3	90	dep	diet	578:581	arg1	0.11					648:651	0.11	648:651	0.11	648:651	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	3	90	dep	diet	578:581	arg1	ratio					588:592	the ratio	584:592	the ratio between amylose and amylopectin of the two diets	584:641	Pigs were fed a tapioca starch (TS) diet or a pea starch (PS) diet (the ratio between amylose and amylopectin of the two diets were 0.11 and 0.44, respectively) for 44 days.
35337551	5	91	dep	enriched	1147:1154	arg1	while					1184:1188	while	1184:1188	while	1184:1188	In addition, analysis of microbial functions showed that 11 carbohydrate-active enzymes, such as GH73, AA3, and AA6, were enriched in the PS group (P < 0.05), while 26 other enzymes, such as GH2, GH35, and GH53, were enriched in the TS group (P < 0.05).
35337551	1	92	theme	different	252:260	arg1	sources					269:275	different starch sources	252:275	different starch sources	252:275	This study integrated metagenomics and metabolomics to evaluate the effects of diets with different starch sources on the microbial community, metabolic functions, and resultant metabolites in the cecum of finishing pigs.
35761655	3	0	theme	biological	288:297	arg1	bioactivity					299:309	biological bioactivity	288:309	biological bioactivity	288:309	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	4	1	theme	polyhedral	448:457	arg1	surface					472:478	polyhedral or spherical surface	448:478	polyhedral or spherical surface	448:478	This study showed that PLS consisted of mixed population of granules with polyhedral or spherical surface.
35761655	8	2	theme	altered	907:913	arg1	composition					915:925	specifically altered composition	894:925	specifically altered composition of gut microbiota	894:943	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	9	3	with	mice	1106:1109	arg1	diet					1142:1145	high-fat high-cholesterol diet	1116:1145	high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD)	1116:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	10	4	theme	Turicibacter	1378:1389	arg1	abundance					1330:1338	the abundance	1326:1338	the abundance of Lactobacillus, Bifidobacterium and Turicibacter	1326:1389	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	3	5	dep	structure	261:269	arg1	the					257:259	the	257:259	the	257:259	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	6	6	theme	chains	718:723	arg1	proportion					695:704	fairly high proportion	683:704	fairly high proportion of B1 short chains	683:723	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	1	7	theme	Pueraria	164:171	arg1	lobata					173:178	Pueraria lobata	164:178	Pueraria lobata	164:178	The dried roots of Pueraria lobata (Willd.)
35761655	10	8	theme	Lactobacillus	1343:1355	arg1	abundance					1330:1338	the abundance	1326:1338	the abundance of Lactobacillus, Bifidobacterium and Turicibacter	1326:1389	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	0	9	theme	fatty	116:120	arg1	disease					128:134	non-alcoholic fatty liver disease	102:134	non-alcoholic fatty liver disease in mice	102:142	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	4	10	theme	spherical	462:470	arg1	surface					472:478	polyhedral or spherical surface	448:478	polyhedral or spherical surface	448:478	This study showed that PLS consisted of mixed population of granules with polyhedral or spherical surface.
35761655	7	11	theme	viscosity	842:850	arg1	stability					825:833	high hot stability	816:833	high hot stability of the viscosity	816:850	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	5	12	theme	molecular	542:550	arg1	Da					574:575	1.93 × 107 Da	563:575	1.93 × 107 Da	563:575	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	5	12	theme	molecular	542:550	arg1	weight					552:557	the molecular weight	538:557	the molecular weight	538:557	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	0	13	dep	high-fat	63:70	arg1	high-cholesterol					72:87	high-cholesterol	72:87	high-cholesterol	72:87	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	0	14	theme	non-alcoholic	102:114	arg1	disease					128:134	non-alcoholic fatty liver disease	102:134	non-alcoholic fatty liver disease in mice	102:142	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	6	15	theme	B1	709:710	arg1	chains					718:723	B1 short chains	709:723	B1 short chains	709:723	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	10	16	theme	protective	1202:1211	arg1	effect					1213:1218	The protective effect	1198:1218	The protective effect of PLS	1198:1225	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	8	17	theme	microbiota	934:943	arg1	composition					915:925	specifically altered composition	894:925	specifically altered composition of gut microbiota	894:943	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	8	17	theme	microbiota	934:943	arg1	production					959:968	increased production	949:968	increased production of SCFAs	949:977	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	10	18	theme	dysbiosis	1284:1292	arg1	amelioration					1247:1258	amelioration	1247:1258	amelioration of NAFLD-associated gut dysbiosis	1247:1292	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	7	19	theme	PLS	763:765	arg1	%					777:777	38.51%	772:777	38.51%	772:777	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	7	19	theme	PLS	763:765	arg1	power					754:758	The solubility and swelling power	726:758	The solubility and swelling power of PLS	726:765	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	1	20	theme	lobata	173:178	arg1	roots					155:159	The dried roots	145:159	The dried roots of Pueraria lobata (Willd.)	145:187	The dried roots of Pueraria lobata (Willd.)
35761655	0	21	theme	liver	122:126	arg1	disease					128:134	non-alcoholic fatty liver disease	102:134	non-alcoholic fatty liver disease in mice	102:142	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	5	22	theme	×	568:568	arg1	Da					574:575	1.93 × 107 Da	563:575	1.93 × 107 Da	563:575	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	5	22	theme	×	568:568	arg1	weight					552:557	the molecular weight	538:557	the molecular weight	538:557	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	7	23	theme	high	816:819	arg1	stability					825:833	high hot stability	816:833	high hot stability of the viscosity	816:850	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	6	24	theme	polymerization	623:636	arg1	rate					638:641	an average polymerization rate	612:641	an average polymerization rate	612:641	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	9	25	from	inflammation	1054:1065	arg1	mice					1106:1109	mice	1106:1109	mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD)	1106:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	0	26	theme	lobata	9:14	arg1	starch					16:21	Pueraria lobata starch	0:21	Pueraria lobata starch	0:21	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	4	27	theme	granules	434:441	arg1	population					420:429	mixed population	414:429	mixed population of granules with polyhedral or spherical surface	414:478	This study showed that PLS consisted of mixed population of granules with polyhedral or spherical surface.
35761655	8	28	theme	increased	949:957	arg1	production					959:968	increased production	949:968	increased production of SCFAs	949:977	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	6	29	theme	high	690:693	arg1	proportion					695:704	fairly high proportion	683:704	fairly high proportion of B1 short chains	683:723	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	6	30	theme	average	615:621	arg1	rate					638:641	an average polymerization rate	612:641	an average polymerization rate	612:641	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	0	31	theme	Pueraria	0:7	arg1	starch					16:21	Pueraria lobata starch	0:21	Pueraria lobata starch	0:21	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	9	32	theme	hepatic	1068:1074	arg1	steatosis					1076:1084	hepatic steatosis	1068:1084	hepatic steatosis	1068:1084	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	9	33	theme	non-alcoholic	1155:1167	arg1	NAFLD					1190:1194	NAFLD	1190:1194	NAFLD	1190:1194	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	9	33	theme	non-alcoholic	1155:1167	arg1	disease					1181:1187	non-alcoholic fatty liver disease	1155:1187	non-alcoholic fatty liver disease (NAFLD)	1155:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	7	34	theme	solubility	730:739	arg1	%					777:777	38.51%	772:777	38.51%	772:777	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	7	34	theme	solubility	730:739	arg1	power					754:758	The solubility and swelling power	726:758	The solubility and swelling power of PLS	726:765	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	10	35	theme	Bifidobacterium	1358:1372	arg1	abundance					1330:1338	the abundance	1326:1338	the abundance of Lactobacillus, Bifidobacterium and Turicibacter	1326:1389	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	6	36	theme	short	712:716	arg1	chains					718:723	B1 short chains	709:723	B1 short chains	709:723	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	8	37	theme	In	853:854	arg1	fermentation					862:873	In vitro fermentation	853:873	In vitro fermentation of PLS	853:880	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	10	38	theme	PLS	1223:1225	arg1	effect					1213:1218	The protective effect	1198:1218	The protective effect of PLS	1198:1225	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	9	39	theme	high-fat	1116:1123	arg1	diet					1142:1145	high-fat high-cholesterol diet	1116:1145	high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD)	1116:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	1	40	dep	roots	155:159	arg1	Willd					181:185	Willd	181:185	Willd	181:185	The dried roots of Pueraria lobata (Willd.)
35761655	8	41	dep	In	853:854	arg1	vitro					856:860	vitro	856:860	vitro	856:860	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	9	42	from	steatosis	1076:1084	arg1	mice					1106:1109	mice	1106:1109	mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD)	1106:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	6	43	theme	branching	591:599	arg1	degree					601:606	a branching degree	589:606	a branching degree	589:606	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	11	44	theme	functional	1468:1477	arg1	use					1452:1454	the use	1448:1454	the use of PLS	1448:1461	The results would support the use of PLS as a functional prebiotic for protecting against NAFLD.
35761655	11	44	theme	functional	1468:1477	arg1	prebiotic					1479:1487	a functional prebiotic	1466:1487	a functional prebiotic for protecting against NAFLD	1466:1516	The results would support the use of PLS as a functional prebiotic for protecting against NAFLD.
35761655	0	45	theme	gut	33:35	arg1	microbiota					37:46	gut microbiota	33:46	gut microbiota	33:46	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	3	46	dep	P.	314:315	arg1	lobata					317:322	lobata	317:322	lobata	317:322	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	4	47	theme	mixed	414:418	arg1	population					420:429	mixed population	414:429	mixed population of granules with polyhedral or spherical surface	414:478	This study showed that PLS consisted of mixed population of granules with polyhedral or spherical surface.
35761655	3	48	theme	starch	324:329	arg1	physiology					272:281	physiology	272:281	physiology	272:281	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	3	48	theme	starch	324:329	arg1	structure					261:269	structure	261:269	structure	261:269	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	3	48	theme	starch	324:329	arg1	bioactivity					299:309	biological bioactivity	288:309	biological bioactivity	288:309	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	0	49	theme	high-fat	63:70	arg1	diet					89:92	high-fat high-cholesterol diet	63:92	high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice	63:142	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	2	50	theme	medicinal	207:215	arg1	herb					217:220	an edible medicinal herb	197:220	an edible medicinal herb	197:220	Ohwi as an edible medicinal herb are enriched with starch.
35761655	5	51	theme	resistant	505:513	arg1	starch					515:520	resistant starch	505:520	resistant starch	505:520	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	8	52	theme	SCFAs	973:977	arg1	composition					915:925	specifically altered composition	894:925	specifically altered composition of gut microbiota	894:943	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	8	52	theme	SCFAs	973:977	arg1	production					959:968	increased production	949:968	increased production of SCFAs	949:977	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	0	53	from	disease	128:134	arg1	mice					139:142	mice	139:142	mice	139:142	Pueraria lobata starch regulates gut microbiota and alleviates high-fat high-cholesterol diet induced non-alcoholic fatty liver disease in mice.
35761655	2	54	theme	edible	200:205	arg1	herb					217:220	an edible medicinal herb	197:220	an edible medicinal herb	197:220	Ohwi as an edible medicinal herb are enriched with starch.
35761655	5	55	theme	starch	515:520	arg1	content					494:500	The apparent content	481:500	The apparent content of resistant starch	481:520	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	5	55	theme	starch	515:520	arg1	%					531:531	23.14%	526:531	23.14%	526:531	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	8	56	theme	gut	930:932	arg1	microbiota					934:943	gut microbiota	930:943	gut microbiota	930:943	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	8	57	theme	prebiotic	1000:1008	arg1	effect					1010:1015	a potential prebiotic effect	988:1015	a potential prebiotic effect	988:1015	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	8	58	theme	PLS	878:880	arg1	fermentation					862:873	In vitro fermentation	853:873	In vitro fermentation of PLS	853:880	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	11	59	theme	PLS	1459:1461	arg1	use					1452:1454	the use	1448:1454	the use of PLS	1448:1461	The results would support the use of PLS as a functional prebiotic for protecting against NAFLD.
35761655	11	59	theme	PLS	1459:1461	arg1	prebiotic					1479:1487	a functional prebiotic	1466:1487	a functional prebiotic for protecting against NAFLD	1466:1516	The results would support the use of PLS as a functional prebiotic for protecting against NAFLD.
35761655	6	60	theme	%	661:661	arg1	rate					638:641	an average polymerization rate	612:641	an average polymerization rate	612:641	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	6	60	theme	%	661:661	arg1	degree					601:606	a branching degree	589:606	a branching degree	589:606	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	9	61	from	dyslipidemia	1090:1101	arg1	mice					1106:1109	mice	1106:1109	mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD)	1106:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	6	62	theme	%	650:650	arg1	rate					638:641	an average polymerization rate	612:641	an average polymerization rate	612:641	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	6	62	theme	%	650:650	arg1	degree					601:606	a branching degree	589:606	a branching degree	589:606	PLS showed a branching degree and an average polymerization rate of 2.06% and 20.74%, respectively, with fairly high proportion of B1 short chains.
35761655	8	63	theme	potential	990:998	arg1	effect					1010:1015	a potential prebiotic effect	988:1015	a potential prebiotic effect	988:1015	In vitro fermentation of PLS resulted in specifically altered composition of gut microbiota and increased production of SCFAs, showing a potential prebiotic effect.
35761655	9	64	theme	liver	1175:1179	arg1	NAFLD					1190:1194	NAFLD	1190:1194	NAFLD	1190:1194	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	9	64	theme	liver	1175:1179	arg1	disease					1181:1187	non-alcoholic fatty liver disease	1155:1187	non-alcoholic fatty liver disease (NAFLD)	1155:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	10	65	theme	gut	1280:1282	arg1	dysbiosis					1284:1292	NAFLD-associated gut dysbiosis	1263:1292	NAFLD-associated gut dysbiosis	1263:1292	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	7	66	theme	hot	821:823	arg1	stability					825:833	high hot stability	816:833	high hot stability of the viscosity	816:850	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	7	67	theme	swelling	745:752	arg1	%					777:777	38.51%	772:777	38.51%	772:777	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	7	67	theme	swelling	745:752	arg1	power					754:758	The solubility and swelling power	726:758	The solubility and swelling power of PLS	726:765	The solubility and swelling power of PLS were 38.51% and 28.10 g/g, respectively, showing high hot stability of the viscosity.
35761655	10	68	theme	NAFLD-associated	1263:1278	arg1	dysbiosis					1284:1292	NAFLD-associated gut dysbiosis	1263:1292	NAFLD-associated gut dysbiosis	1263:1292	The protective effect of PLS was associated with amelioration of NAFLD-associated gut dysbiosis through specifically increasing the abundance of Lactobacillus, Bifidobacterium and Turicibacter, and decreasing Desulfovibrio.
35761655	1	69	theme	dried	149:153	arg1	roots					155:159	The dried roots	145:159	The dried roots of Pueraria lobata (Willd.)	145:187	The dried roots of Pueraria lobata (Willd.)
35761655	9	70	theme	fatty	1169:1173	arg1	NAFLD					1190:1194	NAFLD	1190:1194	NAFLD	1190:1194	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	9	70	theme	fatty	1169:1173	arg1	disease					1181:1187	non-alcoholic fatty liver disease	1155:1187	non-alcoholic fatty liver disease (NAFLD)	1155:1195	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	9	71	dep	high-fat	1116:1123	arg1	high-cholesterol					1125:1140	high-cholesterol	1125:1140	high-cholesterol	1125:1140	Moreover, PLS remarkably alleviated inflammation, hepatic steatosis and dyslipidemia in mice with high-fat high-cholesterol diet induced non-alcoholic fatty liver disease (NAFLD).
35761655	3	72	theme	P.	314:315	arg1	starch					324:329	P. lobata starch	314:329	P. lobata starch (PLS)	314:335	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	3	72	theme	P.	314:315	arg1	PLS					332:334	PLS	332:334	PLS	332:334	However, the structure, physiology, and biological bioactivity of P. lobata starch (PLS) has not yet been fully investigated.
35761655	5	73	theme	apparent	485:492	arg1	content					494:500	The apparent content	481:500	The apparent content of resistant starch	481:520	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	5	73	theme	apparent	485:492	arg1	%					531:531	23.14%	526:531	23.14%	526:531	The apparent content of resistant starch was 23.14%, and the molecular weight was 1.93 × 107 Da.
35761655	4	74	with	granules	434:441	arg1	surface					472:478	polyhedral or spherical surface	448:478	polyhedral or spherical surface	448:478	This study showed that PLS consisted of mixed population of granules with polyhedral or spherical surface.
36219969	12	0	theme	positive	1899:1906	arg1	results					1908:1914	The positive results	1895:1914	The positive results	1895:1914	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	4	1	theme	Schiff	645:650	arg1	reaction					657:664	Schiff base reaction	645:664	Schiff base reaction	645:664	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	7	2	theme	inhibition	1224:1233	arg1	kinetics					1235:1242	the performance, enzyme reaction kinetics and inhibition kinetics	1178:1242	the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase	1178:1276	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	9	3	theme	%	1536:1536	arg1	RSD					1526:1528	RSD	1526:1528	RSD of 4.3% (n = 10)	1526:1545	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	7	4	theme	kinetics	1211:1218	arg1	kinetics					1235:1242	the performance, enzyme reaction kinetics and inhibition kinetics	1178:1242	the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase	1178:1276	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	6	5	theme	functional	896:905	arg1	composite					930:938	the prepared composite	917:938	the prepared composite	917:938	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	5	theme	functional	896:905	arg1	groups					907:912	functional groups	896:912	functional groups	896:912	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	6	dep	Fourier	998:1004	arg1	transform					1006:1014	transform	1006:1014	transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS)	1006:1120	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	7	7	theme	performance	1182:1192	arg1	kinetics					1235:1242	the performance, enzyme reaction kinetics and inhibition kinetics	1178:1242	the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase	1178:1276	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	12	8	theme	enzyme	2113:2118	arg1	inhibitors					2120:2129	enzyme inhibitors	2113:2129	enzyme inhibitors	2113:2129	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	4	9	theme	quinine	717:723	arg1	groups					725:730	the residual quinine groups	704:730	the residual quinine groups in the coating	704:745	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	12	10	theme	reliable	2079:2086	arg1	method					2066:2071	the proposed screening method	2043:2071	the proposed screening method	2043:2071	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	12	10	theme	reliable	2079:2086	arg1	approach					2088:2095	a reliable approach	2077:2095	a reliable approach for discovering enzyme inhibitors from TCMs	2077:2139	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	4	11	theme	enzyme	771:776	arg1	molecules					778:786	enzyme molecules	771:786	enzyme molecules	771:786	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	6	12	theme	total	1036:1040	arg1	spectroscopy					1054:1065	infrared-Attenuated total reflectance spectroscopy	1016:1065	infrared-Attenuated total reflectance spectroscopy (FTIR-ATR)	1016:1076	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	12	theme	total	1036:1040	arg1	FTIR-ATR					1068:1075	FTIR-ATR	1068:1075	FTIR-ATR	1068:1075	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	7	13	theme	capillary	1137:1145	arg1	electrophoresis					1147:1161	capillary electrophoresis	1137:1161	capillary electrophoresis (CE) analysis	1137:1175	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	7	13	theme	capillary	1137:1145	arg1	CE					1164:1165	CE	1164:1165	CE	1164:1165	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	2	14	theme	α-glucosidase	305:317	arg1	immobilization					319:332	α-glucosidase immobilization	305:332	α-glucosidase immobilization	305:332	In this work, polyvinylidene fluoride (PVDF) membrane was employed as the carrier for α-glucosidase immobilization.
36219969	1	15	theme	various	200:206	arg1	materials					208:216	various materials	200:216	various materials	200:216	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	9	16	with	%	1436:1436	arg1	RSD					1526:1528	RSD	1526:1528	RSD of 4.3% (n = 10)	1526:1545	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	3	17	theme	acid	380:383	arg1	co-polymerization					352:368	the co-polymerization	348:368	the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES)	348:429	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	10	18	theme	Michaelis-Menten	1552:1567	arg1	Km					1579:1580	Km	1579:1580	Km	1579:1580	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	10	18	theme	Michaelis-Menten	1552:1567	arg1	constant					1569:1576	The Michaelis-Menten constant	1548:1576	The Michaelis-Menten constant (Km) of immobilized enzyme	1548:1603	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	10	18	theme	Michaelis-Menten	1552:1567	arg1	4.16 mM					1626:1632	4.16 mM	1626:1632	4.16 mM	1626:1632	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	11	19	theme	inhibitory	1874:1883	arg1	activity					1885:1892	the strongest inhibitory activity	1860:1892	the strongest inhibitory activity	1860:1892	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	11	20	theme	medicines	1802:1810	arg1	kinds					1773:1777	13 kinds	1770:1777	13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity	1770:1892	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	12	21	theme	proposed	2047:2054	arg1	method					2066:2071	the proposed screening method	2043:2071	the proposed screening method	2043:2071	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	12	21	theme	proposed	2047:2054	arg1	approach					2088:2095	a reliable approach	2077:2095	a reliable approach for discovering enzyme inhibitors from TCMs	2077:2139	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	6	22	theme	X-ray	1083:1087	arg1	spectroscopy					1103:1114	X-ray photoelectron spectroscopy	1083:1114	X-ray photoelectron spectroscopy (XPS)	1083:1120	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	22	theme	X-ray	1083:1087	arg1	XPS					1117:1119	XPS	1117:1119	XPS	1117:1119	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	3	23	theme	nanospheres	491:501	arg1	coating					503:509	a hierarchical layer-colloidal nanospheres coating	460:509	a hierarchical layer-colloidal nanospheres coating	460:509	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	1	24	theme	catechol-amine	129:142	arg1	strategy					187:194	an effective modification strategy	161:194	an effective modification strategy for various materials	161:216	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	1	24	theme	catechol-amine	129:142	arg1	co-deposition					144:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	9	25	contain	possessed	1393:1401	arg2	reusability					1408:1418	good reusability	1403:1418	good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10)	1403:1545	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	9	25	contain	possessed	1393:1401	arg1	it					1390:1391	it	1390:1391	it	1390:1391	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	3	26	theme	hierarchical	462:473	arg1	coating					503:509	a hierarchical layer-colloidal nanospheres coating	460:509	a hierarchical layer-colloidal nanospheres coating	460:509	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	0	27	theme	Tannic	0:5	arg1	co-deposition					39:51	Tannic acid-aminopropyltriethoxysilane co-deposition	0:51	Tannic acid-aminopropyltriethoxysilane co-deposition	0:51	Tannic acid-aminopropyltriethoxysilane co-deposition modified polymer membrane for α-glucosidase immobilization.
36219969	9	28	theme	relative	1449:1456	arg1	activity					1458:1465	initial relative activity	1441:1465	initial relative activity after 11 recycles	1441:1483	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	12	29	theme	polymer	2022:2028	arg1	membrane					2030:2037	chemically inert polymer membrane	2005:2037	chemically inert polymer membrane	2005:2037	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	8	30	dep	temperature	1351:1361	arg1	value					1370:1374	value	1370:1374	value	1370:1374	The immobilized enzyme exhibited the enhanced tolerance to temperature and pH value.
36219969	12	31	theme	mild	1971:1974	arg1	method					1994:1999	a simple and mild functionalization method	1958:1999	a simple and mild functionalization method for chemically inert polymer membrane	1958:2037	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	12	31	theme	mild	1971:1974	arg1	co-deposition					1940:1952	TA/APTES co-deposition	1931:1952	TA/APTES co-deposition	1931:1952	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	6	32	theme	electron	971:978	arg1	SEM					992:994	SEM	992:994	SEM	992:994	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	32	theme	electron	971:978	arg1	Fourier					998:1004	Fourier	998:1004	Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS)	998:1120	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	32	theme	electron	971:978	arg1	microscopy					980:989	scanning electron microscopy	962:989	scanning electron microscopy (SEM)	962:995	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	1	33	theme	effective	164:172	arg1	strategy					187:194	an effective modification strategy	161:194	an effective modification strategy for various materials	161:216	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	1	33	theme	effective	164:172	arg1	co-deposition					144:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	4	34	theme	PVDF	623:626	arg1	membrane					628:635	the modified PVDF membrane	610:635	the modified PVDF membrane	610:635	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	10	35	theme	immobilized	1586:1596	arg1	enzyme					1598:1603	immobilized enzyme	1586:1603	immobilized enzyme	1586:1603	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	2	36	theme	polyvinylidene	233:246	arg1	carrier					293:299	the carrier	289:299	the carrier for α-glucosidase immobilization	289:332	In this work, polyvinylidene fluoride (PVDF) membrane was employed as the carrier for α-glucosidase immobilization.
36219969	2	36	theme	polyvinylidene	233:246	arg1	membrane					264:271	polyvinylidene fluoride (PVDF) membrane	233:271	polyvinylidene fluoride (PVDF) membrane	233:271	In this work, polyvinylidene fluoride (PVDF) membrane was employed as the carrier for α-glucosidase immobilization.
36219969	0	37	theme	polymer	62:68	arg1	membrane					70:77	polymer membrane	62:77	polymer membrane	62:77	Tannic acid-aminopropyltriethoxysilane co-deposition modified polymer membrane for α-glucosidase immobilization.
36219969	7	38	theme	α-glucosidase	1264:1276	arg1	kinetics					1235:1242	the performance, enzyme reaction kinetics and inhibition kinetics	1178:1242	the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase	1178:1276	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	11	39	theme	Chinese	1794:1800	arg1	medicines					1802:1810	traditional Chinese medicines	1782:1810	traditional Chinese medicines (TCMs)	1782:1817	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	11	39	theme	Chinese	1794:1800	arg1	TCMs					1813:1816	TCMs	1813:1816	TCMs	1813:1816	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	3	40	theme	PVDF	543:546	arg1	membrane					548:555	PVDF membrane	543:555	PVDF membrane	543:555	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	7	41	theme	enzyme	1195:1200	arg1	kinetics					1211:1218	enzyme reaction kinetics	1195:1218	enzyme reaction kinetics	1195:1218	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	12	42	theme	simple	1960:1965	arg1	method					1994:1999	a simple and mild functionalization method	1958:1999	a simple and mild functionalization method for chemically inert polymer membrane	1958:2037	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	12	42	theme	simple	1960:1965	arg1	co-deposition					1940:1952	TA/APTES co-deposition	1931:1952	TA/APTES co-deposition	1931:1952	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	6	43	theme	prepared	921:928	arg1	composite					930:938	the prepared composite	917:938	the prepared composite	917:938	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	44	theme	infrared-Attenuated	1016:1034	arg1	spectroscopy					1054:1065	infrared-Attenuated total reflectance spectroscopy	1016:1065	infrared-Attenuated total reflectance spectroscopy (FTIR-ATR)	1016:1076	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	44	theme	infrared-Attenuated	1016:1034	arg1	FTIR-ATR					1068:1075	FTIR-ATR	1068:1075	FTIR-ATR	1068:1075	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	4	45	theme	addition	678:685	arg1	reaction					687:694	Michael addition reaction	670:694	Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules	670:786	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	7	46	theme	reaction	1202:1209	arg1	kinetics					1211:1218	enzyme reaction kinetics	1195:1218	enzyme reaction kinetics	1195:1218	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	4	47	theme	base	652:655	arg1	reaction					657:664	Schiff base reaction	645:664	Schiff base reaction	645:664	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	10	48	theme	acarbose	1653:1660	arg1	value					1644:1648	IC50 value	1639:1648	IC50 value of acarbose	1639:1660	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	6	49	dep	morphology	881:890	arg1	The					877:879	The	877:879	The	877:879	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	4	50	from	groups	725:730	arg1	molecules					778:786	enzyme molecules	771:786	enzyme molecules	771:786	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	4	50	from	groups	725:730	arg1	coating					739:745	the coating	735:745	the coating	735:745	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	8	51	theme	immobilized	1296:1306	arg1	enzyme					1308:1313	The immobilized enzyme	1292:1313	The immobilized enzyme	1292:1313	The immobilized enzyme exhibited the enhanced tolerance to temperature and pH value.
36219969	8	52	theme	enhanced	1329:1336	arg1	tolerance					1338:1346	the enhanced tolerance	1325:1346	the enhanced tolerance to temperature and pH value	1325:1374	The immobilized enzyme exhibited the enhanced tolerance to temperature and pH value.
36219969	4	53	theme	residual	708:715	arg1	groups					725:730	the residual quinine groups	704:730	the residual quinine groups in the coating	704:745	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	7	54	theme	electrophoresis	1147:1161	arg1	analysis					1168:1175	capillary electrophoresis (CE) analysis	1137:1175	capillary electrophoresis (CE) analysis	1137:1175	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	1	55	theme	modification	174:185	arg1	strategy					187:194	an effective modification strategy	161:194	an effective modification strategy for various materials	161:216	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	1	55	theme	modification	174:185	arg1	co-deposition					144:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	6	56	theme	reflectance	1042:1052	arg1	spectroscopy					1054:1065	infrared-Attenuated total reflectance spectroscopy	1016:1065	infrared-Attenuated total reflectance spectroscopy (FTIR-ATR)	1016:1076	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	56	theme	reflectance	1042:1052	arg1	FTIR-ATR					1068:1075	FTIR-ATR	1068:1075	FTIR-ATR	1068:1075	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	3	57	theme	tannic	373:378	arg1	TA					386:387	TA	386:387	TA	386:387	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	3	57	theme	tannic	373:378	arg1	acid					380:383	tannic acid	373:383	tannic acid (TA)	373:388	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	3	58	theme	co-polymerization	352:368	arg1	virtue					338:343	virtue	338:343	virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES)	338:429	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	3	58	theme	co-polymerization	352:368	arg1	hydrolysis					439:448	the hydrolysis	435:448	the hydrolysis of APTES	435:457	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	5	59	theme	immobilization	822:835	arg1	procedure					837:845	the immobilization procedure	818:845	the immobilization procedure	818:845	Several parameters affecting the immobilization procedure were investigated thoroughly.
36219969	11	60	theme	Sanguisorba	1832:1842	arg1	Radix					1844:1848	Sanguisorba Radix	1832:1848	Sanguisorba Radix	1832:1848	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	9	61	theme	good	1403:1406	arg1	reusability					1408:1418	good reusability	1403:1418	good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10)	1403:1545	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	4	62	theme	amino	755:759	arg1	groups					761:766	the amino groups	751:766	the amino groups in enzyme molecules	751:786	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	5	63	theme	Several	789:795	arg1	parameters					797:806	Several parameters	789:806	Several parameters affecting the immobilization procedure	789:845	Several parameters affecting the immobilization procedure were investigated thoroughly.
36219969	12	64	theme	screening	2056:2064	arg1	method					2066:2071	the proposed screening method	2043:2071	the proposed screening method	2043:2071	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	12	64	theme	screening	2056:2064	arg1	approach					2088:2095	a reliable approach	2077:2095	a reliable approach for discovering enzyme inhibitors from TCMs	2077:2139	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	6	65	theme	photoelectron	1089:1101	arg1	spectroscopy					1103:1114	X-ray photoelectron spectroscopy	1083:1114	X-ray photoelectron spectroscopy (XPS)	1083:1120	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	65	theme	photoelectron	1089:1101	arg1	XPS					1117:1119	XPS	1117:1119	XPS	1117:1119	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	11	66	theme	strongest	1864:1872	arg1	activity					1885:1892	the strongest inhibitory activity	1860:1892	the strongest inhibitory activity	1860:1892	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	12	67	theme	inert	2016:2020	arg1	membrane					2030:2037	chemically inert polymer membrane	2005:2037	chemically inert polymer membrane	2005:2037	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	1	68	theme	Mussel-inspired	113:127	arg1	strategy					187:194	an effective modification strategy	161:194	an effective modification strategy for various materials	161:216	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	1	68	theme	Mussel-inspired	113:127	arg1	co-deposition					144:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition	113:156	Mussel-inspired catechol-amine co-deposition is an effective modification strategy for various materials.
36219969	3	69	theme	3-aminopropyltriethoxysilane	394:421	arg1	co-polymerization					352:368	the co-polymerization	348:368	the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES)	348:429	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	0	70	theme	acid-aminopropyltriethoxysilane	7:37	arg1	co-deposition					39:51	Tannic acid-aminopropyltriethoxysilane co-deposition	0:51	Tannic acid-aminopropyltriethoxysilane co-deposition	0:51	Tannic acid-aminopropyltriethoxysilane co-deposition modified polymer membrane for α-glucosidase immobilization.
36219969	9	71	theme	initial	1441:1447	arg1	activity					1458:1465	initial relative activity	1441:1465	initial relative activity after 11 recycles	1441:1483	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	4	72	theme	modified	614:621	arg1	membrane					628:635	the modified PVDF membrane	610:635	the modified PVDF membrane	610:635	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	3	73	theme	layer-colloidal	475:489	arg1	coating					503:509	a hierarchical layer-colloidal nanospheres coating	460:509	a hierarchical layer-colloidal nanospheres coating	460:509	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	9	74	theme	activity	1458:1465	arg1	%					1436:1436	77.1%	1432:1436	77.1% of initial relative activity after 11 recycles	1432:1483	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	9	74	theme	activity	1458:1465	arg1	reproducibility					1505:1519	batch-to-batch reproducibility	1490:1519	batch-to-batch reproducibility with RSD of 4.3% (n = 10)	1490:1545	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	9	74	theme	activity	1458:1465	arg1	activity					1458:1465	initial relative activity	1441:1465	initial relative activity after 11 recycles	1441:1483	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	12	75	theme	functionalization	1976:1992	arg1	method					1994:1999	a simple and mild functionalization method	1958:1999	a simple and mild functionalization method for chemically inert polymer membrane	1958:2037	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	12	75	theme	functionalization	1976:1992	arg1	co-deposition					1940:1952	TA/APTES co-deposition	1931:1952	TA/APTES co-deposition	1931:1952	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	10	76	theme	enzyme	1598:1603	arg1	Km					1579:1580	Km	1579:1580	Km	1579:1580	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	10	76	theme	enzyme	1598:1603	arg1	constant					1569:1576	The Michaelis-Menten constant	1548:1576	The Michaelis-Menten constant (Km) of immobilized enzyme	1548:1603	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	10	76	theme	enzyme	1598:1603	arg1	4.16 mM					1626:1632	4.16 mM	1626:1632	4.16 mM	1626:1632	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	2	77	theme	fluoride	248:255	arg1	carrier					293:299	the carrier	289:299	the carrier for α-glucosidase immobilization	289:332	In this work, polyvinylidene fluoride (PVDF) membrane was employed as the carrier for α-glucosidase immobilization.
36219969	2	77	theme	fluoride	248:255	arg1	membrane					264:271	polyvinylidene fluoride (PVDF) membrane	233:271	polyvinylidene fluoride (PVDF) membrane	233:271	In this work, polyvinylidene fluoride (PVDF) membrane was employed as the carrier for α-glucosidase immobilization.
36219969	7	78	theme	PVDF-immobilized	1247:1262	arg1	α-glucosidase					1264:1276	PVDF-immobilized α-glucosidase	1247:1276	PVDF-immobilized α-glucosidase	1247:1276	Combined with capillary electrophoresis (CE) analysis, the performance, enzyme reaction kinetics and inhibition kinetics of PVDF-immobilized α-glucosidase were studied.
36219969	6	79	theme	scanning	962:969	arg1	SEM					992:994	SEM	992:994	SEM	992:994	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	79	theme	scanning	962:969	arg1	Fourier					998:1004	Fourier	998:1004	Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS)	998:1120	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	79	theme	scanning	962:969	arg1	microscopy					980:989	scanning electron microscopy	962:989	scanning electron microscopy (SEM)	962:995	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	11	80	theme	traditional	1782:1792	arg1	medicines					1802:1810	traditional Chinese medicines	1782:1810	traditional Chinese medicines (TCMs)	1782:1817	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	11	80	theme	traditional	1782:1792	arg1	TCMs					1813:1816	TCMs	1813:1816	TCMs	1813:1816	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	0	81	theme	α-glucosidase	83:95	arg1	immobilization					97:110	α-glucosidase immobilization	83:110	α-glucosidase immobilization	83:110	Tannic acid-aminopropyltriethoxysilane co-deposition modified polymer membrane for α-glucosidase immobilization.
36219969	3	82	theme	APTES	453:457	arg1	virtue					338:343	virtue	338:343	virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES)	338:429	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	3	82	theme	APTES	453:457	arg1	hydrolysis					439:448	the hydrolysis	435:448	the hydrolysis of APTES	435:457	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	3	83	theme	membrane	548:555	arg1	surface					532:538	the surface	528:538	the surface of PVDF membrane	528:555	By virtue of the co-polymerization of tannic acid (TA) and 3-aminopropyltriethoxysilane (APTES) and the hydrolysis of APTES, a hierarchical layer-colloidal nanospheres coating was decorated on the surface of PVDF membrane.
36219969	9	84	theme	batch-to-batch	1490:1503	arg1	reproducibility					1505:1519	batch-to-batch reproducibility	1490:1519	batch-to-batch reproducibility with RSD of 4.3% (n = 10)	1490:1545	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	6	85	theme	composite	930:938	arg1	composite					930:938	the prepared composite	917:938	the prepared composite	917:938	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	85	theme	composite	930:938	arg1	morphology					881:890	morphology	881:890	morphology	881:890	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	6	85	theme	composite	930:938	arg1	groups					907:912	functional groups	896:912	functional groups	896:912	The morphology and functional groups of the prepared composite were characterized by scanning electron microscopy (SEM), Fourier transform infrared-Attenuated total reflectance spectroscopy (FTIR-ATR), and X-ray photoelectron spectroscopy (XPS).
36219969	12	86	theme	TA/APTES	1931:1938	arg1	method					1994:1999	a simple and mild functionalization method	1958:1999	a simple and mild functionalization method for chemically inert polymer membrane	1958:2037	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	12	86	theme	TA/APTES	1931:1938	arg1	co-deposition					1940:1952	TA/APTES co-deposition	1931:1952	TA/APTES co-deposition	1931:1952	The positive results suggested that TA/APTES co-deposition was a simple and mild functionalization method for chemically inert polymer membrane and the proposed screening method was a reliable approach for discovering enzyme inhibitors from TCMs.
36219969	9	87	with	reproducibility	1505:1519	arg1	RSD					1526:1528	RSD	1526:1528	RSD of 4.3% (n = 10)	1526:1545	In addition, it possessed good reusability maintaining 77.1% of initial relative activity after 11 recycles, and batch-to-batch reproducibility with RSD of 4.3% (n = 10).
36219969	4	88	theme	Michael	670:676	arg1	reaction					687:694	Michael addition reaction	670:694	Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules	670:786	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	2	89	theme	PVDF	258:261	arg1	carrier					293:299	the carrier	289:299	the carrier for α-glucosidase immobilization	289:332	In this work, polyvinylidene fluoride (PVDF) membrane was employed as the carrier for α-glucosidase immobilization.
36219969	2	89	theme	PVDF	258:261	arg1	membrane					264:271	polyvinylidene fluoride (PVDF) membrane	233:271	polyvinylidene fluoride (PVDF) membrane	233:271	In this work, polyvinylidene fluoride (PVDF) membrane was employed as the carrier for α-glucosidase immobilization.
36219969	10	90	theme	IC50	1639:1642	arg1	value					1644:1648	IC50 value	1639:1648	IC50 value of acarbose	1639:1660	The Michaelis-Menten constant (Km) of immobilized enzyme was calculated to be 4.16 mM, and IC50 value of acarbose was 0.10 µM.
36219969	11	91	theme	potential	1744:1752	arg1	inhibitors					1754:1763	potential inhibitors	1744:1763	potential inhibitors	1744:1763	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	11	92	theme	PVDF-immobilized	1688:1703	arg1	α-glucosidase					1705:1717	the PVDF-immobilized α-glucosidase	1684:1717	the PVDF-immobilized α-glucosidase	1684:1717	Finally, the PVDF-immobilized α-glucosidase was applied to screening potential inhibitors from 13 kinds of traditional Chinese medicines (TCMs), among which Sanguisorba Radix exhibited the strongest inhibitory activity.
36219969	4	93	from	groups	761:766	arg1	molecules					778:786	enzyme molecules	771:786	enzyme molecules	771:786	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
36219969	4	93	from	groups	761:766	arg1	coating					739:745	the coating	735:745	the coating	735:745	Subsequently, α-glucosidase was covalently bound to the modified PVDF membrane through Schiff base reaction and Michael addition reaction between the residual quinine groups in the coating and the amino groups in enzyme molecules.
35112687	5	0	theme	Subsequent	695:704	arg1	digestions					718:727	Subsequent oro-gastric digestions	695:727	Subsequent oro-gastric digestions	695:727	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	1	1	theme	density	139:145	arg1	Breads					122:127	Breads	122:127	Breads of higher density	122:145	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	5	2	theme	oro-gastric	706:716	arg1	digestions					718:727	Subsequent oro-gastric digestions	695:727	Subsequent oro-gastric digestions	695:727	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	8	3	theme	gastric	1400:1406	arg1	phase					1408:1412	the gastric phase	1396:1412	the gastric phase	1396:1412	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	7	4	theme	mediating	1032:1040	arg1	role					1042:1045	a mediating role	1030:1045	a mediating role for gastric digestion in the relationship between bread density and GI	1030:1116	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	7	5	theme	in	1004:1005	arg1	studies					1012:1018	in vivo studies	1004:1018	in vivo studies	1004:1018	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	2	6	theme	work	300:303	arg1	aim					288:290	The aim	284:290	The aim of this work	284:303	The aim of this work was to gain a better understanding of the relationship between bread density, oral and gastric digestion, and GI.
35112687	7	7	dep	in	1004:1005	arg1	vivo					1007:1010	vivo	1007:1010	vivo	1007:1010	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	8	8	theme	salivary	1304:1311	arg1	α-amylase					1313:1321	salivary α-amylase	1304:1321	salivary α-amylase	1304:1321	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	3	9	theme	French	485:490	arg1	baguettes					492:500	French baguettes	485:500	industrial-style and traditional-style French baguettes (similar composition, different densities)	446:543	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	8	10	theme	starch	1326:1331	arg1	digestion					1333:1341	starch digestion	1326:1341	starch digestion	1326:1341	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	0	11	theme	breads	74:79	arg1	index					65:69	the glycaemic index	51:69	the glycaemic index of breads with different composition or structure	51:119	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	6	12	theme	bread	911:915	arg1	density					917:923	higher bread density	904:923	higher bread density	904:923	They further showed that higher bread density led to a lower hydrolysis rate.
35112687	3	13	dep	baguettes	492:500	arg1	composition					511:521	similar composition	503:521	similar composition	503:521	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	3	13	dep	baguettes	492:500	arg1	densities					534:542	different densities	524:542	different densities	524:542	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	7	14	theme	bread	1097:1101	arg1	density					1103:1109	bread density	1097:1109	bread density	1097:1109	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	8	15	theme	phase	1408:1412	arg1	role					1388:1391	the possible role	1375:1391	the possible role of the gastric phase in determining the GI	1375:1434	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	5	16	theme	extensive	770:778	arg1	digestion					787:795	extensive starch digestion	770:795	extensive starch digestion at the gastric stage by salivary α-amylase	770:838	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	5	17	theme	gastric	804:810	arg1	stage					812:816	the gastric stage	800:816	the gastric stage	800:816	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	1	18	theme	lower	155:159	arg1	index					171:175	lower glycaemic index	155:175	lower glycaemic index (GI)	155:180	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	1	18	theme	lower	155:159	arg1	GI					178:179	GI	178:179	GI	178:179	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	0	19	theme	different	86:94	arg1	composition					96:106	different composition	86:106	different composition	86:106	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	3	20	theme	traditional-style	467:483	arg1	baguettes					492:500	French baguettes	485:500	industrial-style and traditional-style French baguettes (similar composition, different densities)	446:543	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	1	21	theme	glycaemic	161:169	arg1	index					171:175	lower glycaemic index	155:175	lower glycaemic index (GI)	155:180	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	1	21	theme	glycaemic	161:169	arg1	GI					178:179	GI	178:179	GI	178:179	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	5	22	theme	dynamic	740:746	arg1	system					748:753	the dynamic system	736:753	the dynamic system DIDGI®	736:760	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	7	23	theme	starch	1157:1162	arg1	proportion					1164:1173	the starch proportion	1153:1173	the starch proportion that remains to be hydrolysed in the small intestine	1153:1226	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	5	24	theme	published	863:871	arg1	data					873:876	recently published data	854:876	recently published data	854:876	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	2	25	theme	better	319:324	arg1	understanding					326:338	a better understanding	317:338	a better understanding of the relationship between bread density, oral and gastric digestion, and GI	317:416	The aim of this work was to gain a better understanding of the relationship between bread density, oral and gastric digestion, and GI.
35112687	2	26	theme	oral	383:386	arg1	digestion					400:408	oral and gastric digestion	383:408	oral and gastric digestion	383:408	The aim of this work was to gain a better understanding of the relationship between bread density, oral and gastric digestion, and GI.
35112687	6	27	theme	higher	904:909	arg1	density					917:923	higher bread density	904:923	higher bread density	904:923	They further showed that higher bread density led to a lower hydrolysis rate.
35112687	5	28	with	line	844:847	arg1	data					873:876	recently published data	854:876	recently published data	854:876	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	3	29	dep	studied	437:443	arg1	baguettes					492:500	French baguettes	485:500	industrial-style and traditional-style French baguettes (similar composition, different densities)	446:543	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	3	29	dep	studied	437:443	arg1	baguette					562:569	whole-wheat baguette	550:569	whole-wheat baguette	550:569	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	0	30	theme	starch	41:46	arg1	digestion					28:36	gastric digestion	20:36	gastric digestion of starch	20:46	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	0	31	theme	gastric	20:26	arg1	digestion					28:36	gastric digestion	20:36	gastric digestion of starch	20:46	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	5	32	theme	system	748:753	arg1	DIDGI®					755:760	the dynamic system DIDGI®	736:760	the dynamic system DIDGI®	736:760	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	0	33	with	breads	74:79	arg1	structure					111:119	structure	111:119	structure	111:119	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	0	33	with	breads	74:79	arg1	composition					96:106	different composition	86:106	different composition	86:106	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	4	34	theme	lower	685:689	arg1	GI					691:692	a lower GI	683:692	a lower GI	683:692	In vitro GI predictions confirmed that, for an identical composition, higher bread density was associated with a lower GI.
35112687	7	35	from	repercussions	1136:1148	arg1	proportion					1164:1173	the starch proportion	1153:1173	the starch proportion that remains to be hydrolysed in the small intestine	1153:1226	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	2	36	theme	bread	368:372	arg1	density					374:380	bread density	368:380	bread density	368:380	The aim of this work was to gain a better understanding of the relationship between bread density, oral and gastric digestion, and GI.
35112687	8	37	theme	scientific	1262:1271	arg1	knowledge					1273:1281	the scientific knowledge	1258:1281	the scientific knowledge of the importance of salivary α-amylase to starch digestion	1258:1341	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	7	38	with	concurrence	961:971	arg1	those					995:999	those	995:999	those	995:999	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	3	39	theme	similar	503:509	arg1	composition					511:521	similar composition	503:521	similar composition	503:521	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	3	39	theme	similar	503:509	arg1	densities					534:542	different densities	524:542	different densities	524:542	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	4	40	theme	In	572:573	arg1	predictions					584:594	In vitro GI predictions	572:594	In vitro GI predictions	572:594	In vitro GI predictions confirmed that, for an identical composition, higher bread density was associated with a lower GI.
35112687	7	41	theme	gastric	1051:1057	arg1	digestion					1059:1067	gastric digestion	1051:1067	gastric digestion	1051:1067	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	1	42	theme	slower	248:253	arg1	digestion					273:281	a slower intestinal starch digestion	246:281	a slower intestinal starch digestion	246:281	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	8	43	theme	importance	1290:1299	arg1	knowledge					1273:1281	the scientific knowledge	1258:1281	the scientific knowledge of the importance of salivary α-amylase to starch digestion	1258:1341	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	2	44	theme	relationship	347:358	arg1	understanding					326:338	a better understanding	317:338	a better understanding of the relationship between bread density, oral and gastric digestion, and GI	317:416	The aim of this work was to gain a better understanding of the relationship between bread density, oral and gastric digestion, and GI.
35112687	0	45	theme	digestion	28:36	arg1	contribution					4:15	The contribution	0:15	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure	0:119	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	1	46	theme	intestinal	255:264	arg1	digestion					273:281	a slower intestinal starch digestion	246:281	a slower intestinal starch digestion	246:281	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	7	47	theme	results	982:988	arg1	concurrence					961:971	The concurrence	957:971	The concurrence	957:971	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	8	48	theme	special	1354:1360	arg1	attention					1362:1370	special attention	1354:1370	special attention	1354:1370	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	4	49	theme	GI	581:582	arg1	predictions					584:594	In vitro GI predictions	572:594	In vitro GI predictions	572:594	In vitro GI predictions confirmed that, for an identical composition, higher bread density was associated with a lower GI.
35112687	1	50	theme	starch	266:271	arg1	digestion					273:281	a slower intestinal starch digestion	246:281	a slower intestinal starch digestion	246:281	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	3	51	theme	different	524:532	arg1	composition					511:521	similar composition	503:521	similar composition	503:521	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	3	51	theme	different	524:532	arg1	densities					534:542	different densities	524:542	different densities	524:542	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	4	52	theme	bread	649:653	arg1	density					655:661	higher bread density	642:661	higher bread density	642:661	In vitro GI predictions confirmed that, for an identical composition, higher bread density was associated with a lower GI.
35112687	2	53	theme	gastric	392:398	arg1	digestion					400:408	oral and gastric digestion	383:408	oral and gastric digestion	383:408	The aim of this work was to gain a better understanding of the relationship between bread density, oral and gastric digestion, and GI.
35112687	4	54	dep	In	572:573	arg1	vitro					575:579	vitro	575:579	vitro	575:579	In vitro GI predictions confirmed that, for an identical composition, higher bread density was associated with a lower GI.
35112687	4	55	theme	higher	642:647	arg1	density					655:661	higher bread density	642:661	higher bread density	642:661	In vitro GI predictions confirmed that, for an identical composition, higher bread density was associated with a lower GI.
35112687	5	56	dep	extensive	770:778	arg1	starch					780:785	starch	780:785	starch	780:785	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	8	57	theme	possible	1379:1386	arg1	role					1388:1391	the possible role	1375:1391	the possible role of the gastric phase in determining the GI	1375:1434	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	8	58	theme	α-amylase	1313:1321	arg1	importance					1290:1299	the importance	1286:1299	the importance of salivary α-amylase to starch digestion	1286:1341	This study therefore adds to the scientific knowledge of the importance of salivary α-amylase to starch digestion, and draws special attention to the possible role of the gastric phase in determining the GI.
35112687	1	59	theme	higher	132:137	arg1	density					139:145	higher density	132:145	higher density	132:145	Breads of higher density exhibit lower glycaemic index (GI) both in vivo and in vitro, a phenomenon generally attributed to a slower intestinal starch digestion.
35112687	7	60	theme	small	1212:1216	arg1	intestine					1218:1226	the small intestine	1208:1226	the small intestine	1208:1226	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	6	61	theme	hydrolysis	940:949	arg1	rate					951:954	a lower hydrolysis rate	932:954	a lower hydrolysis rate	932:954	They further showed that higher bread density led to a lower hydrolysis rate.
35112687	0	62	theme	glycaemic	55:63	arg1	index					65:69	the glycaemic index	51:69	the glycaemic index of breads with different composition or structure	51:119	The contribution of gastric digestion of starch to the glycaemic index of breads with different composition or structure.
35112687	5	63	theme	salivary	821:828	arg1	α-amylase					830:838	salivary α-amylase	821:838	salivary α-amylase	821:838	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	5	64	from	stage	812:816	arg1	digestion					787:795	extensive starch digestion	770:795	extensive starch digestion at the gastric stage by salivary α-amylase	770:838	Subsequent oro-gastric digestions, using the dynamic system DIDGI®, showed extensive starch digestion at the gastric stage by salivary α-amylase, in line with recently published data.
35112687	4	65	theme	identical	619:627	arg1	composition					629:639	an identical composition	616:639	an identical composition	616:639	In vitro GI predictions confirmed that, for an identical composition, higher bread density was associated with a lower GI.
35112687	6	66	theme	lower	934:938	arg1	rate					951:954	a lower hydrolysis rate	932:954	a lower hydrolysis rate	932:954	They further showed that higher bread density led to a lower hydrolysis rate.
35112687	7	67	from	role	1042:1045	arg1	relationship					1076:1087	the relationship	1072:1087	the relationship between bread density and GI	1072:1116	The concurrence of these results with those of in vivo studies, suggests a mediating role for gastric digestion in the relationship between bread density and GI, possibly via the repercussions on the starch proportion that remains to be hydrolysed in the small intestine.
35112687	3	68	theme	whole-wheat	550:560	arg1	baguette					562:569	whole-wheat baguette	550:569	whole-wheat baguette	550:569	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
35112687	3	69	theme	industrial-style	446:461	arg1	baguettes					492:500	French baguettes	485:500	industrial-style and traditional-style French baguettes (similar composition, different densities)	446:543	Three breads were studied: industrial-style and traditional-style French baguettes (similar composition, different densities), and whole-wheat baguette.
36575259	11	0	theme	integrated	3109:3118	arg1	approach					3134:3141	an integrated nanocomposite approach	3106:3141	an integrated nanocomposite approach	3106:3141	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	7	1	theme	nanocomposite	1599:1611	arg1	beads					1613:1617	The freeze-dried chitosan-titania nanocomposite beads	1565:1617	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag	1565:1640	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	8	2	theme	experimental	2223:2234	arg1	concentration					2282:2294	concentration	2282:2294	concentration of feed solution	2282:2311	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	2	theme	experimental	2223:2234	arg1	conditions					2236:2245	varying experimental conditions	2215:2245	varying experimental conditions such as flow rate, bed height, and concentration of feed solution	2215:2311	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	2	theme	experimental	2223:2234	arg1	rate					2260:2263	flow rate	2255:2263	flow rate	2255:2263	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	2	theme	experimental	2223:2234	arg1	height					2270:2275	bed height	2266:2275	bed height	2266:2275	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	3	theme	photocatalytic	2063:2076	arg1	study					2078:2082	Continuous flow adsorption cum photocatalytic study	2032:2082	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads	2032:2123	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	2	4	theme	present	363:369	arg1	work					371:374	the present work	359:374	the present work	359:374	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	9	5	from	beads	2429:2433	arg1	experiment					2488:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	1	6	theme	anthropogenic	254:266	arg1	activities					268:277	various anthropogenic activities	246:277	various anthropogenic activities	246:277	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	7	7	theme	freeze-dried	1569:1580	arg1	beads					1613:1617	The freeze-dried chitosan-titania nanocomposite beads	1565:1617	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag	1565:1640	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	3	8	theme	textural	721:728	arg1	features					749:756	their structural, textural, and morphological features	703:756	their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy	703:984	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	8	9	theme	flow	2255:2258	arg1	rate					2260:2263	flow rate	2255:2263	flow rate	2255:2263	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	2	10	link	sol-gel-derived	377:391	arg1	nanocomposites					404:417	sol-gel-derived functional nanocomposites	377:417	sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites	377:516	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	5	11	theme	contaminated	1273:1284	arg1	water					1286:1290	the model contaminated water	1263:1290	the model contaminated water	1263:1290	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	6	12	theme	photocatalytic	1515:1528	arg1	efficiencies					1530:1541	the adsorption cum photocatalytic efficiencies	1496:1541	the adsorption cum photocatalytic efficiencies of the nanocomposite	1496:1562	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	1	13	theme	increased	176:184	arg1	discharge					186:194	the increased discharge	172:194	the increased discharge of nitrates to the natural water resources	172:237	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	11	14	theme	nanocomposite	3120:3132	arg1	approach					3134:3141	an integrated nanocomposite approach	3106:3141	an integrated nanocomposite approach	3106:3141	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	9	15	theme	adsorption	2458:2467	arg1	experiment					2488:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	9	16	dep	concentration	2533:2545	arg1	5.0 mL min-1					2591:2602	5.0 mL min-1	2591:2602	5.0 mL min-1	2591:2602	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	5	17	theme	Adsorption/photocatalytic	1111:1135	arg1	reduction					1137:1145	Adsorption/photocatalytic reduction	1111:1145	Adsorption/photocatalytic reduction of nitrates	1111:1157	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	9	18	theme	photocatalysis	2473:2486	arg1	experiment					2488:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	7	19	from	reduction	1982:1990	arg1	solution					2022:2029	an aqueous solution	2011:2029	an aqueous solution	2011:2029	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	0	20	from	based	81:85	arg1	nanocomposite					116:128	chitosan-titanium dioxide nanocomposite	90:128	chitosan-titanium dioxide nanocomposite	90:128	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	9	21	theme	Nitrate	2314:2320	arg1	efficiency					2332:2341	Nitrate reduction efficiency	2314:2341	Nitrate reduction efficiency of 87.6%	2314:2350	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	7	22	theme	composition	1828:1838	arg1	beads					1810:1814	the oven dried beads	1795:1814	the oven dried beads of the same composition	1795:1838	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	5	23	theme	water	1286:1290	arg1	concentration					1246:1258	the nitrate concentration	1234:1258	the nitrate concentration of the model contaminated water	1234:1290	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	11	24	from	reduction	3054:3062	arg1	water/wastewater					3081:3096	water/wastewater	3081:3096	water/wastewater through an integrated nanocomposite approach	3081:3141	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	1	25	theme	natural	215:221	arg1	resources					229:237	the natural water resources	211:237	the natural water resources	211:237	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	7	26	theme	reduction	1731:1739	arg1	%					1759:1759	photocatalytic reduction capability of ~ 95%	1716:1759	photocatalytic reduction capability of ~ 95% (under sunlight for 2 h)	1716:1784	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	9	27	theme	continuous	2442:2451	arg1	experiment					2488:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	3	28	theme	X-ray	764:768	arg1	analysis					782:789	X-ray diffraction analysis	764:789	X-ray diffraction analysis	764:789	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	11	29	theme	cum	2937:2939	arg1	efficiency					2974:2983	the adsorption cum photocatalytic nitrate reduction efficiency	2922:2983	the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material	2922:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	11	30	theme	adsorption	2926:2935	arg1	efficiency					2974:2983	the adsorption cum photocatalytic nitrate reduction efficiency	2922:2983	the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material	2922:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	3	31	theme	area	870:873	arg1	analysis					875:882	BET surface area analysis	858:882	BET surface area analysis	858:882	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	2	32	theme	beads	563:567	arg1	form					555:558	the form	551:558	the form of beads	551:567	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	8	33	theme	solution	2304:2311	arg1	concentration					2282:2294	concentration	2282:2294	concentration of feed solution	2282:2311	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	33	theme	solution	2304:2311	arg1	rate					2260:2263	flow rate	2255:2263	flow rate	2255:2263	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	33	theme	solution	2304:2311	arg1	height					2270:2275	bed height	2266:2275	bed height	2266:2275	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	3	34	theme	infrared	810:817	arg1	spectroscopy					819:830	infrared spectroscopy	810:830	infrared spectroscopy	810:830	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	3	35	theme	BET	858:860	arg1	analysis					875:882	BET surface area analysis	858:882	BET surface area analysis	858:882	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	2	36	theme	-coated	486:492	arg1	nanocomposites					503:516	-coated chitosan nanocomposites	486:516	-coated chitosan nanocomposites	486:516	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	10	37	theme	representative	2607:2620	arg1	experiment					2650:2659	A representative fixed-bed column adsorption experiment	2605:2659	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala	2605:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	0	38	theme	photocatalyst	57:69	arg1	based					81:85	an integrated photocatalyst adsorbent based	43:85	an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite	43:128	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	1	39	theme	huge	156:159	arg1	concern					161:167	a huge concern	154:167	a huge concern on the increased discharge of nitrates to the natural water resources	154:237	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	3	40	theme	electron	894:901	arg1	microscopy					903:912	Scanning electron microscopy	885:912	Scanning electron microscopy	885:912	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	4	41	theme	titania	1014:1020	arg1	species					1022:1028	doped titania species	1008:1028	doped titania species	1008:1028	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
36575259	0	42	from	reduction	18:26	arg1	water					31:35	water	31:35	water	31:35	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	10	43	dep	along	2848:2852	arg1	with					2854:2857	with	2854:2857	with	2854:2857	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	5	44	theme	nanocomposite	1200:1212	arg1	beads					1214:1218	functional nanocomposite beads	1189:1218	functional nanocomposite beads	1189:1218	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	10	45	theme	Kerala	2776:2781	arg1	District					2764:2771	the Palakkad District	2751:2771	the Palakkad District of Kerala	2751:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	1	46	from	concern	161:167	arg1	discharge					186:194	the increased discharge	172:194	the increased discharge of nitrates to the natural water resources	172:237	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	7	47	theme	oven	1799:1802	arg1	beads					1810:1814	the oven dried beads	1795:1814	the oven dried beads of the same composition	1795:1838	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	5	48	theme	photocatalytic	1341:1354	arg1	study					1356:1360	photocatalytic study	1341:1360	photocatalytic study	1341:1360	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	0	49	theme	chitosan-titanium	90:106	arg1	nanocomposite					116:128	chitosan-titanium dioxide nanocomposite	90:128	chitosan-titanium dioxide nanocomposite	90:128	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	7	50	theme	nitrate	1995:2001	arg1	ions					2003:2006	nitrate ions	1995:2006	nitrate ions	1995:2006	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	3	51	theme	electron	928:935	arg1	microscopy					937:946	Transmission electron microscopy	915:946	Transmission electron microscopy	915:946	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	4	52	theme	uniform	989:995	arg1	coating					997:1003	A uniform coating	987:1003	A uniform coating of doped titania species on the chitosan porous structure	987:1061	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
36575259	10	53	theme	%	2834:2834	arg1	reduction					2819:2827	nitrate reduction	2811:2827	nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well	2811:2913	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	10	53	theme	%	2834:2834	arg1	efficiency					2836:2845	85.9% efficiency	2830:2845	85.9% efficiency	2830:2845	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	10	54	theme	column	2632:2637	arg1	experiment					2650:2659	A representative fixed-bed column adsorption experiment	2605:2659	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala	2605:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	9	55	theme	reduction	2322:2330	arg1	efficiency					2332:2341	Nitrate reduction efficiency	2314:2341	Nitrate reduction efficiency of 87.6%	2314:2350	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	8	56	theme	adsorption	2048:2057	arg1	study					2078:2082	Continuous flow adsorption cum photocatalytic study	2032:2082	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads	2032:2123	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	9	57	theme	bed	2559:2561	arg1	12 cm					2570:2574	bed height 12 cm	2559:2574	bed height 12 cm	2559:2574	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	2	58	theme	N	454:454	arg1	TiO2					481:484	TiO2	481:484	TiO2	481:484	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	2	58	theme	N	454:454	arg1	dioxide					472:478	nitrogen (N)-doped titanium dioxide	444:478	nitrogen (N)-doped titanium dioxide (TiO2)	444:485	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	10	59	theme	dried	2681:2685	arg1	beads					2701:2705	oven dried nanocomposite beads	2676:2705	oven dried nanocomposite beads	2676:2705	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	0	60	theme	Efficient	0:8	arg1	reduction					18:26	Efficient nitrate reduction	0:26	Efficient nitrate reduction in water	0:35	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	3	61	theme	X-ray	953:957	arg1	spectroscopy					973:984	X-ray photoelectron spectroscopy	953:984	X-ray photoelectron spectroscopy	953:984	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	2	62	theme	nitrogen	444:451	arg1	TiO2					481:484	TiO2	481:484	TiO2	481:484	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	2	62	theme	nitrogen	444:451	arg1	dioxide					472:478	nitrogen (N)-doped titanium dioxide	444:478	nitrogen (N)-doped titanium dioxide (TiO2)	444:485	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	5	63	theme	definite	1386:1393	arg1	period					1400:1405	a definite time period	1384:1405	a definite time period	1384:1405	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	9	64	theme	%	2350:2350	arg1	capacity					2370:2377	an adsorption capacity	2356:2377	an adsorption capacity of 7.9 mg g-1	2356:2391	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	9	64	theme	%	2350:2350	arg1	efficiency					2332:2341	Nitrate reduction efficiency	2314:2341	Nitrate reduction efficiency of 87.6%	2314:2350	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	6	65	theme	adsorption	1500:1509	arg1	efficiencies					1530:1541	the adsorption cum photocatalytic efficiencies	1496:1541	the adsorption cum photocatalytic efficiencies of the nanocomposite	1496:1562	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	1	66	theme	serious	292:298	arg1	pollution					314:322	serious environmental pollution	292:322	serious environmental pollution	292:322	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	11	67	theme	photocatalytic	2941:2954	arg1	efficiency					2974:2983	the adsorption cum photocatalytic nitrate reduction efficiency	2922:2983	the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material	2922:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	7	68	theme	same	1823:1826	arg1	composition					1828:1838	the same composition	1819:1838	the same composition	1819:1838	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	8	69	theme	oven-dried	2094:2103	arg1	beads					2119:2123	the oven-dried nanocomposite beads	2090:2123	the oven-dried nanocomposite beads	2090:2123	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	4	70	theme	species	1022:1028	arg1	coating					997:1003	A uniform coating	987:1003	A uniform coating of doped titania species on the chitosan porous structure	987:1061	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
36575259	4	71	theme	chitosan	1037:1044	arg1	structure					1053:1061	the chitosan porous structure	1033:1061	the chitosan porous structure	1033:1061	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
36575259	2	72	from	reduction	600:608	arg1	water					625:629	water	625:629	water	625:629	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	3	73	theme	structural	709:718	arg1	features					749:756	their structural, textural, and morphological features	703:756	their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy	703:984	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	6	74	theme	significant	1474:1484	arg1	effect					1486:1491	a significant effect	1472:1491	a significant effect	1472:1491	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	1	75	theme	associated	328:337	arg1	effects					347:353	associated harmful effects	328:353	associated harmful effects	328:353	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	7	76	theme	0.5 mol	1630:1636	arg1	Ag					1639:1640	0.5 mol% Ag	1630:1640	0.5 mol% Ag	1630:1640	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	8	77	theme	experimental	2166:2177	arg1	setup					2179:2183	an experimental setup	2163:2183	an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution	2163:2311	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	10	78	theme	significant	2861:2871	arg1	rate					2881:2884	a significant removal rate	2859:2884	nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well	2811:2913	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	9	79	theme	7.9 mg g-1	2382:2391	arg1	capacity					2370:2377	an adsorption capacity	2356:2377	an adsorption capacity of 7.9 mg g-1	2356:2391	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	9	79	theme	7.9 mg g-1	2382:2391	arg1	efficiency					2332:2341	Nitrate reduction efficiency	2314:2341	Nitrate reduction efficiency of 87.6%	2314:2350	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	1	80	theme	water	223:227	arg1	resources					229:237	the natural water resources	211:237	the natural water resources	211:237	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	8	81	dep	the	2151:2153	arg1	help					2155:2158	help	2155:2158	help	2155:2158	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	3	82	theme	synthesized	648:658	arg1	beads					674:678	The synthesized nanocomposite beads	644:678	The synthesized nanocomposite beads	644:678	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	10	83	theme	groundwater	2717:2727	arg1	sample					2729:2734	a real groundwater sample	2710:2734	a real groundwater sample collected from the Palakkad District of Kerala	2710:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	2	84	theme	sol-gel-derived	377:391	arg1	nanocomposites					404:417	sol-gel-derived functional nanocomposites	377:417	sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites	377:516	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	2	85	theme	-doped	456:461	arg1	TiO2					481:484	TiO2	481:484	TiO2	481:484	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	2	85	theme	-doped	456:461	arg1	dioxide					472:478	nitrogen (N)-doped titanium dioxide	444:478	nitrogen (N)-doped titanium dioxide (TiO2)	444:485	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	7	86	theme	chitosan-titania	1582:1597	arg1	beads					1613:1617	The freeze-dried chitosan-titania nanocomposite beads	1565:1617	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag	1565:1640	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	4	87	theme	electrostatic	1084:1096	arg1	interaction					1098:1108	electrostatic interaction	1084:1108	electrostatic interaction	1084:1108	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
36575259	6	88	theme	nanocomposite	1550:1562	arg1	efficiencies					1530:1541	the adsorption cum photocatalytic efficiencies	1496:1541	the adsorption cum photocatalytic efficiencies of the nanocomposite	1496:1562	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	1	89	theme	various	246:252	arg1	activities					268:277	various anthropogenic activities	246:277	various anthropogenic activities	246:277	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	10	90	theme	nitrate	2811:2817	arg1	reduction					2819:2827	nitrate reduction	2811:2827	nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well	2811:2913	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	10	90	theme	nitrate	2811:2817	arg1	efficiency					2836:2845	85.9% efficiency	2830:2845	85.9% efficiency	2830:2845	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	7	91	theme	%	1897:1897	arg1	adsorption					1849:1858	adsorption	1849:1858	adsorption	1849:1858	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	7	91	theme	%	1897:1897	arg1	efficiencies					1879:1890	photocatalytic efficiencies	1864:1890	photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively	1864:1967	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	8	92	theme	varying	2215:2221	arg1	concentration					2282:2294	concentration	2282:2294	concentration of feed solution	2282:2311	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	92	theme	varying	2215:2221	arg1	conditions					2236:2245	varying experimental conditions	2215:2245	varying experimental conditions such as flow rate, bed height, and concentration of feed solution	2215:2311	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	92	theme	varying	2215:2221	arg1	rate					2260:2263	flow rate	2255:2263	flow rate	2255:2263	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	8	92	theme	varying	2215:2221	arg1	height					2270:2275	bed height	2266:2275	bed height	2266:2275	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	9	93	theme	inlet	2527:2531	arg1	concentration					2533:2545	an inlet concentration	2524:2545	an inlet concentration of 100 ppm	2524:2556	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	6	94	contain	have	1467:1470	arg1	conditions					1415:1424	Drying conditions	1408:1424	Drying conditions of the nanocomposite beads	1408:1451	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	6	94	contain	have	1467:1470	arg2	effect					1486:1491	a significant effect	1472:1491	a significant effect	1472:1491	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	7	95	contain	containing	1619:1628	arg1	beads					1613:1617	The freeze-dried chitosan-titania nanocomposite beads	1565:1617	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag	1565:1640	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	7	95	contain	containing	1619:1628	arg2	Ag					1639:1640	0.5 mol% Ag	1630:1640	0.5 mol% Ag	1630:1640	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	2	96	theme	nitrates	613:620	arg1	reduction					600:608	the reduction	596:608	the reduction of nitrates in water	596:629	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	7	97	theme	photocatalytic	1864:1877	arg1	efficiencies					1879:1890	photocatalytic efficiencies	1864:1890	photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively	1864:1967	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	9	98	theme	flow	2453:2456	arg1	experiment					2488:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	11	99	theme	nitrates	3067:3074	arg1	reduction					3054:3062	the reduction	3050:3062	the reduction of nitrates from water/wastewater through an integrated nanocomposite approach	3050:3141	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	11	100	theme	material	3017:3024	arg1	efficiency					2974:2983	the adsorption cum photocatalytic nitrate reduction efficiency	2922:2983	the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material	2922:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	9	101	theme	cum	2469:2471	arg1	experiment					2488:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	the continuous flow adsorption cum photocatalysis experiment	2438:2497	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	1	102	theme	nitrates	199:206	arg1	discharge					186:194	the increased discharge	172:194	the increased discharge of nitrates to the natural water resources	172:237	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	7	103	theme	capability	1741:1750	arg1	%					1759:1759	photocatalytic reduction capability of ~ 95%	1716:1759	photocatalytic reduction capability of ~ 95% (under sunlight for 2 h)	1716:1784	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	8	104	theme	bed	2266:2268	arg1	height					2270:2275	bed height	2266:2275	bed height	2266:2275	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	3	105	theme	morphological	735:747	arg1	features					749:756	their structural, textural, and morphological features	703:756	their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy	703:984	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	6	106	theme	Drying	1408:1413	arg1	conditions					1415:1424	Drying conditions	1408:1424	Drying conditions of the nanocomposite beads	1408:1451	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	7	107	theme	photocatalytic	1716:1729	arg1	%					1759:1759	photocatalytic reduction capability of ~ 95%	1716:1759	photocatalytic reduction capability of ~ 95% (under sunlight for 2 h)	1716:1784	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	1	108	theme	harmful	339:345	arg1	effects					347:353	associated harmful effects	328:353	associated harmful effects	328:353	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	8	109	theme	flow	2043:2046	arg1	study					2078:2082	Continuous flow adsorption cum photocatalytic study	2032:2082	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads	2032:2123	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	5	110	theme	nitrates	1150:1157	arg1	reduction					1137:1145	Adsorption/photocatalytic reduction	1111:1145	Adsorption/photocatalytic reduction of nitrates	1111:1157	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	7	111	theme	aqueous	2014:2020	arg1	solution					2022:2029	an aqueous solution	2011:2029	an aqueous solution	2011:2029	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	1	112	dep	activities	268:277	arg1	out					239:241	out	239:241	out	239:241	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	3	113	theme	diffraction	770:780	arg1	analysis					782:789	X-ray diffraction analysis	764:789	X-ray diffraction analysis	764:789	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	5	114	dep	contaminated	1273:1284	arg1	model					1267:1271	model	1267:1271	model	1267:1271	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	8	115	theme	feed	2299:2302	arg1	solution					2304:2311	feed solution	2299:2311	feed solution	2299:2311	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	0	116	theme	adsorbent	71:79	arg1	based					81:85	an integrated photocatalyst adsorbent based	43:85	an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite	43:128	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	7	117	theme	dried	1804:1808	arg1	beads					1810:1814	the oven dried beads	1795:1814	the oven dried beads of the same composition	1795:1838	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	3	118	theme	surface	862:868	arg1	analysis					875:882	BET surface area analysis	858:882	BET surface area analysis	858:882	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	5	119	theme	dark	1322:1325	arg1	condition					1327:1335	dark condition	1322:1335	dark condition	1322:1335	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	6	120	theme	beads	1447:1451	arg1	conditions					1415:1424	Drying conditions	1408:1424	Drying conditions of the nanocomposite beads	1408:1451	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	5	121	theme	functional	1189:1198	arg1	beads					1214:1218	functional nanocomposite beads	1189:1218	functional nanocomposite beads	1189:1218	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	7	122	theme	ions	2003:2006	arg1	reduction					1982:1990	the reduction	1978:1990	the reduction of nitrate ions in an aqueous solution	1978:2029	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	11	123	theme	reduction	2964:2972	arg1	efficiency					2974:2983	the adsorption cum photocatalytic nitrate reduction efficiency	2922:2983	the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material	2922:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	4	124	theme	doped	1008:1012	arg1	species					1022:1028	doped titania species	1008:1028	doped titania species	1008:1028	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
36575259	9	125	theme	100 ppm	2550:2556	arg1	rate					2586:2589	flow rate	2581:2589	flow rate	2581:2589	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	9	125	theme	100 ppm	2550:2556	arg1	concentration					2533:2545	an inlet concentration	2524:2545	an inlet concentration of 100 ppm	2524:2556	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	9	125	theme	100 ppm	2550:2556	arg1	12 cm					2570:2574	bed height 12 cm	2559:2574	bed height 12 cm	2559:2574	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	11	126	theme	functional	2992:3001	arg1	material					3017:3024	the functional nanocomposite material	2988:3024	the functional nanocomposite material	2988:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	3	127	dep	Fourier	792:798	arg1	transform					800:808	transform	800:808	transform infrared spectroscopy	800:830	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	0	128	theme	dioxide	108:114	arg1	nanocomposite					116:128	chitosan-titanium dioxide nanocomposite	90:128	chitosan-titanium dioxide nanocomposite	90:128	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	3	129	theme	Scanning	885:892	arg1	microscopy					903:912	Scanning electron microscopy	885:912	Scanning electron microscopy	885:912	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	7	130	theme	efficiency	1666:1675	arg1	%					1686:1686	an adsorption efficiency of ~ 43.5%	1652:1686	an adsorption efficiency of ~ 43.5% (under dark for 30 min)	1652:1710	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	10	131	theme	adsorption	2639:2648	arg1	experiment					2650:2659	A representative fixed-bed column adsorption experiment	2605:2659	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala	2605:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	3	132	theme	UV-visible	833:842	arg1	spectroscopy					844:855	UV-visible spectroscopy	833:855	UV-visible spectroscopy	833:855	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	6	133	theme	nanocomposite	1433:1445	arg1	beads					1447:1451	the nanocomposite beads	1429:1451	the nanocomposite beads	1429:1451	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	8	134	theme	Continuous	2032:2041	arg1	study					2078:2082	Continuous flow adsorption cum photocatalytic study	2032:2082	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads	2032:2123	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	2	135	theme	chitosan	494:501	arg1	nanocomposites					503:516	-coated chitosan nanocomposites	486:516	-coated chitosan nanocomposites	486:516	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	10	136	theme	fixed-bed	2622:2630	arg1	experiment					2650:2659	A representative fixed-bed column adsorption experiment	2605:2659	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala	2605:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	8	137	theme	cum	2059:2061	arg1	study					2078:2082	Continuous flow adsorption cum photocatalytic study	2032:2082	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads	2032:2123	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	0	138	theme	nitrate	10:16	arg1	reduction					18:26	Efficient nitrate reduction	0:26	Efficient nitrate reduction in water	0:35	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	9	139	theme	height	2563:2568	arg1	12 cm					2570:2574	bed height 12 cm	2559:2574	bed height 12 cm	2559:2574	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	3	140	theme	Transmission	915:926	arg1	microscopy					937:946	Transmission electron microscopy	915:946	Transmission electron microscopy	915:946	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	4	141	theme	porous	1046:1051	arg1	structure					1053:1061	the chitosan porous structure	1033:1061	the chitosan porous structure	1033:1061	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
36575259	10	142	theme	oven	2676:2679	arg1	beads					2701:2705	oven dried nanocomposite beads	2676:2705	oven dried nanocomposite beads	2676:2705	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	2	143	theme	functional	393:402	arg1	nanocomposites					404:417	sol-gel-derived functional nanocomposites	377:417	sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites	377:516	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	6	144	theme	cum	1511:1513	arg1	efficiencies					1530:1541	the adsorption cum photocatalytic efficiencies	1496:1541	the adsorption cum photocatalytic efficiencies of the nanocomposite	1496:1562	Drying conditions of the nanocomposite beads were found to have a significant effect on the adsorption cum photocatalytic efficiencies of the nanocomposite.
36575259	10	145	theme	promising	2789:2797	arg1	results					2799:2805	promising results	2789:2805	promising results	2789:2805	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	3	146	theme	photoelectron	959:971	arg1	spectroscopy					973:984	X-ray photoelectron spectroscopy	953:984	X-ray photoelectron spectroscopy	953:984	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	11	147	theme	nanocomposite	3003:3015	arg1	material					3017:3024	the functional nanocomposite material	2988:3024	the functional nanocomposite material	2988:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	5	148	theme	time	1395:1398	arg1	period					1400:1405	a definite time period	1384:1405	a definite time period	1384:1405	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	8	149	theme	nanocomposite	2105:2117	arg1	beads					2119:2123	the oven-dried nanocomposite beads	2090:2123	the oven-dried nanocomposite beads	2090:2123	Continuous flow adsorption cum photocatalytic study using the oven-dried nanocomposite beads was also carried out with the help of an experimental setup fabricated in-house and under varying experimental conditions such as flow rate, bed height, and concentration of feed solution.
36575259	1	150	theme	environmental	300:312	arg1	pollution					314:322	serious environmental pollution	292:322	serious environmental pollution	292:322	Globally, there exists a huge concern on the increased discharge of nitrates to the natural water resources out of various anthropogenic activities as it causes serious environmental pollution and associated harmful effects.
36575259	9	151	theme	flow	2581:2584	arg1	rate					2586:2589	flow rate	2581:2589	flow rate	2581:2589	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	7	152	theme	adsorption	1655:1664	arg1	%					1686:1686	an adsorption efficiency of ~ 43.5%	1652:1686	an adsorption efficiency of ~ 43.5% (under dark for 30 min)	1652:1710	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	2	153	theme	titanium	463:470	arg1	TiO2					481:484	TiO2	481:484	TiO2	481:484	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	2	153	theme	titanium	463:470	arg1	dioxide					472:478	nitrogen (N)-doped titanium dioxide	444:478	nitrogen (N)-doped titanium dioxide (TiO2)	444:485	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	2	154	dep	silver	428:433	arg1	nanocomposites					503:516	-coated chitosan nanocomposites	486:516	-coated chitosan nanocomposites	486:516	In the present work, sol-gel-derived functional nanocomposites based on silver (Ag) and nitrogen (N)-doped titanium dioxide (TiO2)-coated chitosan nanocomposites were successfully synthesized in the form of beads, and their application for the reduction of nitrates in water was studied.
36575259	9	155	theme	adsorption	2359:2368	arg1	capacity					2370:2377	an adsorption capacity	2356:2377	an adsorption capacity of 7.9 mg g-1	2356:2391	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	0	156	theme	integrated	46:55	arg1	based					81:85	an integrated photocatalyst adsorbent based	43:85	an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite	43:128	Efficient nitrate reduction in water using an integrated photocatalyst adsorbent based on chitosan-titanium dioxide nanocomposite.
36575259	7	157	theme	UV	1938:1939	arg1	light					1941:1945	UV light	1938:1945	UV light for 2 h	1938:1953	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	7	158	theme	%	1929:1929	arg1	adsorption					1849:1858	adsorption	1849:1858	adsorption	1849:1858	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	7	158	theme	%	1929:1929	arg1	efficiencies					1879:1890	photocatalytic efficiencies	1864:1890	photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively	1864:1967	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	10	159	theme	removal	2873:2879	arg1	rate					2881:2884	a significant removal rate	2859:2884	nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well	2811:2913	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	3	160	theme	nanocomposite	660:672	arg1	beads					674:678	The synthesized nanocomposite beads	644:678	The synthesized nanocomposite beads	644:678	The synthesized nanocomposite beads were characterized for their structural, textural, and morphological features using X-ray diffraction analysis, Fourier transform infrared spectroscopy, UV-visible spectroscopy, BET surface area analysis, Scanning electron microscopy, Transmission electron microscopy, and X-ray photoelectron spectroscopy.
36575259	10	161	theme	nanocomposite	2687:2699	arg1	beads					2701:2705	oven dried nanocomposite beads	2676:2705	oven dried nanocomposite beads	2676:2705	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	5	162	theme	nitrate	1238:1244	arg1	concentration					1246:1258	the nitrate concentration	1234:1258	the nitrate concentration of the model contaminated water	1234:1290	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	11	163	theme	nitrate	2956:2962	arg1	efficiency					2974:2983	the adsorption cum photocatalytic nitrate reduction efficiency	2922:2983	the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material	2922:3024	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	7	164	theme	%	1637:1637	arg1	Ag					1639:1640	0.5 mol% Ag	1630:1640	0.5 mol% Ag	1630:1640	The freeze-dried chitosan-titania nanocomposite beads containing 0.5 mol% Ag exhibited an adsorption efficiency of ~ 43.5% (under dark for 30 min) and photocatalytic reduction capability of ~ 95% (under sunlight for 2 h), whereas the oven dried beads of the same composition exhibits adsorption and photocatalytic efficiencies of 40% (under dark for 30 min) and 70% (under UV light for 2 h) respectively, towards the reduction of nitrate ions in an aqueous solution.
36575259	11	165	from	water/wastewater	3081:3096	arg1	nitrates					3067:3074	nitrates	3067:3074	nitrates from water/wastewater through an integrated nanocomposite approach	3067:3141	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	11	165	from	water/wastewater	3081:3096	arg1	reduction					3054:3062	the reduction	3050:3062	the reduction of nitrates from water/wastewater through an integrated nanocomposite approach	3050:3141	Thus, the adsorption cum photocatalytic nitrate reduction efficiency of the functional nanocomposite material makes them suitable for the reduction of nitrates from water/wastewater through an integrated nanocomposite approach.
36575259	9	166	theme	nanocomposite	2415:2427	arg1	beads					2429:2433	the nanocomposite beads	2411:2433	the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment	2411:2497	Nitrate reduction efficiency of 87.6% and an adsorption capacity of 7.9 mg g-1 were obtained for the nanocomposite beads in the continuous flow adsorption cum photocatalysis experiment for up to 8 h when using an inlet concentration of 100 ppm, bed height 12 cm, and flow rate 5.0 mL min-1.
36575259	5	167	theme	adsorption	1299:1308	arg1	study					1310:1314	an adsorption study	1296:1314	an adsorption study under dark condition	1296:1335	Adsorption/photocatalytic reduction of nitrates was further carried out using functional nanocomposite beads by monitoring the nitrate concentration of the model contaminated water, in an adsorption study under dark condition and photocatalytic study under UV/sunlight for a definite time period.
36575259	10	168	theme	Palakkad	2755:2762	arg1	District					2764:2771	the Palakkad District	2751:2771	the Palakkad District of Kerala	2751:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	10	169	theme	other	2894:2898	arg1	anions					2900:2905	the other anions	2890:2905	the other anions as well	2890:2913	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	10	170	theme	real	2712:2715	arg1	sample					2729:2734	a real groundwater sample	2710:2734	a real groundwater sample collected from the Palakkad District of Kerala	2710:2781	A representative fixed-bed column adsorption experiment performed with oven dried nanocomposite beads in a real groundwater sample collected from the Palakkad District of Kerala shows promising results for nitrate reduction (85.9% efficiency) along with a significant removal rate for the other anions as well.
36575259	4	171	from	coating	997:1003	arg1	structure					1053:1061	the chitosan porous structure	1033:1061	the chitosan porous structure	1033:1061	A uniform coating of doped titania species on the chitosan porous structure was achieved through electrostatic interaction.
35917312	6	0	from	solution	1089:1096	arg1	day					1112:1114	the fourth day	1101:1114	the fourth day	1101:1114	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	6	1	theme	mechanical	929:938	arg1	properties					940:949	mechanical properties	929:949	mechanical properties	929:949	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	4	2	theme	in-vitro	728:735	arg1	release					742:748	in-vitro drug release	728:748	in-vitro drug release analysis	728:757	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	4	3	theme	In-vitro	589:596	arg1	analysis					598:605	In-vitro analysis	589:605	In-vitro analysis	589:605	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	5	4	theme	dissolution	806:816	arg1	capability					818:827	the dissolution capability	802:827	the dissolution capability of the microneedle patch	802:852	In-vivo studies were performed to analyze the dissolution capability of the microneedle patch.
35917312	6	5	dep	reusing	1077:1083	arg1	results					1116:1122	results	1116:1122	results in failure patches	1116:1141	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	9	6	theme	%	1721:1721	arg1	percentage					1695:1704	a dissolution percentage	1681:1704	a dissolution percentage of more than 50% of the original height of microneedle after 7 days	1681:1772	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	1	7	theme	macromolecules	159:172	arg1	diffusion					146:154	transdermal diffusion	134:154	transdermal diffusion of macromolecules	134:172	Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin.
35917312	5	8	theme	In-vivo	760:766	arg1	studies					768:774	In-vivo studies	760:774	In-vivo studies	760:774	In-vivo studies were performed to analyze the dissolution capability of the microneedle patch.
35917312	6	9	theme	rheological	966:976	arg1	properties					978:987	rheological properties	966:987	rheological properties	966:987	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	3	10	theme	chemical	532:539	arg1	composition					541:551	the chemical composition	528:551	the chemical composition	528:551	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	9	11	theme	dissolving	1658:1667	arg1	capable					1634:1640	capable	1634:1640	capable	1634:1640	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	9	12	theme	height	1739:1744	arg1	height					1739:1744	the original height	1726:1744	the original height of microneedle after 7 days	1726:1772	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	9	12	theme	height	1739:1744	arg1	%					1721:1721	more than 50%	1709:1721	more than 50% of the original height of microneedle after 7 days	1709:1772	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	3	13	theme	composition	541:551	arg1	preservation					512:523	preservation	512:523	preservation of the chemical composition after sterilization	512:571	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	0	14	theme	Biodegradable	0:12	arg1	patch					26:30	Biodegradable microneedle patch	0:30	Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle.	0:85	Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle.
35917312	11	15	theme	biodegradable	1901:1913	arg1	patches					1927:1933	chitosan biodegradable microneedle patches	1892:1933	chitosan biodegradable microneedle patches	1892:1933	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	4	16	theme	release	742:748	arg1	analysis					750:757	in-vitro drug release analysis	728:757	in-vitro drug release analysis	728:757	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	7	17	theme	chemical	1178:1185	arg1	studies					1199:1205	chemical composition studies	1178:1205	chemical composition studies	1178:1205	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	4	18	theme	mechanical	650:659	arg1	properties					661:670	in-vitro penetration mechanical properties	629:670	in-vitro penetration mechanical properties	629:670	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	8	19	theme	±	1422:1422	arg1	%					1428:1428	approximately 33.02 ± 3.88%	1402:1428	approximately 33.02 ± 3.88% of the meloxicam	1402:1445	In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days.
35917312	8	19	theme	±	1422:1422	arg1	meloxicam					1437:1445	the meloxicam	1433:1445	the meloxicam	1433:1445	In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days.
35917312	5	20	theme	microneedle	836:846	arg1	patch					848:852	the microneedle patch	832:852	the microneedle patch	832:852	In-vivo studies were performed to analyze the dissolution capability of the microneedle patch.
35917312	5	21	theme	patch	848:852	arg1	capability					818:827	the dissolution capability	802:827	the dissolution capability of the microneedle patch	802:852	In-vivo studies were performed to analyze the dissolution capability of the microneedle patch.
35917312	8	22	theme	In-vitro	1373:1380	arg1	studies					1382:1388	In-vitro studies	1373:1388	In-vitro studies	1373:1388	In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days.
35917312	2	23	theme	microneedle	265:275	arg1	patch					277:281	a biodegradable chitosan microneedle patch	240:281	a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle	240:330	In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested.
35917312	4	24	theme	testing	685:691	arg1	analysis					693:700	compression testing analysis	673:700	compression testing analysis of microneedle patch	673:721	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	0	25	theme	microneedle	14:24	arg1	patch					26:30	Biodegradable microneedle patch	0:30	Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle.	0:85	Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle.
35917312	6	26	theme	significant	1023:1033	arg1	differences					1035:1045	significant differences	1023:1045	significant differences	1023:1045	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	10	27	located	observed	1825:1832	arg2	swelling					1795:1802	swelling	1795:1802	swelling	1795:1802	No abnormal tissue, swelling, or inflammation was observed in the implanted area.
35917312	10	27	located	observed	1825:1832	arg2	tissue					1787:1792	No abnormal tissue	1775:1792	No abnormal tissue	1775:1792	No abnormal tissue, swelling, or inflammation was observed in the implanted area.
35917312	10	27	located	observed	1825:1832	arg2	inflammation					1808:1819	inflammation	1808:1819	inflammation	1808:1819	No abnormal tissue, swelling, or inflammation was observed in the implanted area.
35917312	10	27	located	observed	1825:1832	arg1	area					1851:1854	the implanted area	1837:1854	the implanted area	1837:1854	No abnormal tissue, swelling, or inflammation was observed in the implanted area.
35917312	4	28	theme	microneedle	705:715	arg1	patch					717:721	microneedle patch	705:721	microneedle patch	705:721	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	3	29	theme	polymeric	374:382	arg1	solution					384:391	the polymeric solution	370:391	the polymeric solution	370:391	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	9	30	theme	microneedle	1749:1759	arg1	height					1739:1744	the original height	1726:1744	the original height of microneedle after 7 days	1726:1772	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	1	31	theme	Microneedle	87:97	arg1	source					123:128	a promising source	111:128	a promising source for transdermal diffusion of macromolecules	111:172	Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin.
35917312	1	31	theme	Microneedle	87:97	arg1	patches					99:105	Microneedle patches	87:105	Microneedle patches	87:105	Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin.
35917312	6	32	theme	failure	1127:1133	arg1	patches					1135:1141	failure patches	1127:1141	failure patches	1127:1141	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	3	33	theme	reuse	361:365	arg1	potential					348:356	The potential	344:356	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization	344:571	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	11	34	theme	cattle	2000:2005	arg1	management					1986:1995	pain management	1981:1995	pain management of cattle with positive effects for commercial manufacturing	1981:2056	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	9	35	theme	dissolution	1683:1693	arg1	percentage					1695:1704	a dissolution percentage	1681:1704	a dissolution percentage of more than 50% of the original height of microneedle after 7 days	1681:1772	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	3	36	theme	morphological	448:460	arg1	patches					410:416	the patches	406:416	the patches	406:416	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	36	theme	morphological	448:460	arg1	analysis					462:469	morphological analysis	448:469	morphological analysis of the microneedle patch	448:494	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	7	37	theme	oxide	1260:1264	arg1	sterilization					1236:1248	sterilization	1236:1248	sterilization (ethylene oxide gas) needed for implanting the patches into the skin	1236:1317	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	7	37	theme	oxide	1260:1264	arg1	gas					1266:1268	ethylene oxide gas	1251:1268	ethylene oxide gas	1251:1268	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	11	38	theme	pain	1981:1984	arg1	management					1986:1995	pain management	1981:1995	pain management of cattle with positive effects for commercial manufacturing	1981:2056	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	7	39	theme	Morphological	1144:1156	arg1	characteristics					1158:1172	Morphological characteristics	1144:1172	Morphological characteristics	1144:1172	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	9	40	theme	swelling	1645:1652	arg1	capable					1634:1640	capable	1634:1640	capable	1634:1640	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	1	41	theme	transdermal	134:144	arg1	diffusion					146:154	transdermal diffusion	134:154	transdermal diffusion of macromolecules	134:172	Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin.
35917312	7	42	theme	sterilization	1236:1248	arg1	process					1225:1231	the process	1221:1231	the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin	1221:1317	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	3	43	theme	patch	490:494	arg1	optimization					419:430	optimization	419:430	optimization of fabrication	419:445	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	43	theme	patch	490:494	arg1	analysis					500:507	analysis	500:507	analysis of preservation of the chemical composition after sterilization	500:571	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	43	theme	patch	490:494	arg1	patches					410:416	the patches	406:416	the patches	406:416	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	43	theme	patch	490:494	arg1	analysis					462:469	morphological analysis	448:469	morphological analysis of the microneedle patch	448:494	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	9	44	dep	N.	1572:1573	arg1	demonstrated					1591:1602	demonstrated	1591:1602	demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days	1591:1772	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	4	45	theme	compression	673:683	arg1	analysis					693:700	compression testing analysis	673:700	compression testing analysis of microneedle patch	673:721	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	10	46	theme	implanted	1841:1849	arg1	area					1851:1854	the implanted area	1837:1854	the implanted area	1837:1854	No abnormal tissue, swelling, or inflammation was observed in the implanted area.
35917312	3	47	theme	fabrication	435:445	arg1	optimization					419:430	optimization	419:430	optimization of fabrication	419:445	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	47	theme	fabrication	435:445	arg1	analysis					500:507	analysis	500:507	analysis of preservation of the chemical composition after sterilization	500:571	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	47	theme	fabrication	435:445	arg1	patches					410:416	the patches	406:416	the patches	406:416	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	47	theme	fabrication	435:445	arg1	analysis					462:469	morphological analysis	448:469	morphological analysis of the microneedle patch	448:494	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	9	48	theme	microneedles	1499:1510	arg1	penetration					1480:1490	A full penetration	1473:1490	A full penetration of the microneedles into the skin	1473:1524	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	11	49	with	management	1986:1995	arg1	effects					2021:2027	positive effects	2012:2027	positive effects for commercial manufacturing	2012:2056	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	7	50	theme	ethylene	1251:1258	arg1	oxide					1260:1264	ethylene oxide	1251:1264	ethylene oxide gas	1251:1268	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	9	51	theme	full	1475:1478	arg1	penetration					1480:1490	A full penetration	1473:1490	A full penetration of the microneedles into the skin	1473:1524	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	6	52	theme	fourth	1105:1110	arg1	day					1112:1114	the fourth day	1101:1114	the fourth day	1101:1114	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	3	53	theme	solution	384:391	arg1	reuse					361:365	reuse	361:365	reuse of the polymeric solution	361:391	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	11	54	theme	work	1877:1880	arg1	results					1861:1867	The results	1857:1867	The results of this work	1857:1880	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	6	55	theme	chemical	903:910	arg1	composition					912:922	chemical composition	903:922	chemical composition	903:922	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	8	56	theme	meloxicam	1437:1445	arg1	%					1428:1428	approximately 33.02 ± 3.88%	1402:1428	approximately 33.02 ± 3.88% of the meloxicam	1402:1445	In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days.
35917312	8	56	theme	meloxicam	1437:1445	arg1	meloxicam					1437:1445	the meloxicam	1433:1445	the meloxicam	1433:1445	In-vitro studies showed that approximately 33.02 ± 3.88% of the meloxicam was released over 7 days.
35917312	3	57	theme	microneedle	478:488	arg1	patch					490:494	the microneedle patch	474:494	the microneedle patch	474:494	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	0	58	theme	meloxicam	48:56	arg1	delivery					36:43	delivery	36:43	delivery of meloxicam	36:56	Biodegradable microneedle patch for delivery of meloxicam for managing pain in cattle.
35917312	6	59	theme	solution	1005:1012	arg1	properties					978:987	rheological properties	966:987	rheological properties	966:987	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	9	60	theme	microneedle	1609:1619	arg1	patches					1621:1627	microneedle patches	1609:1627	microneedle patches	1609:1627	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	7	61	theme	composition	1187:1197	arg1	studies					1199:1205	chemical composition studies	1178:1205	chemical composition studies	1178:1205	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	7	62	theme	microneedle	1352:1362	arg1	patches					1364:1370	microneedle patches	1352:1370	microneedle patches	1352:1370	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	1	63	theme	promising	113:121	arg1	source					123:128	a promising source	111:128	a promising source for transdermal diffusion of macromolecules	111:172	Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin.
35917312	1	63	theme	promising	113:121	arg1	patches					99:105	Microneedle patches	87:105	Microneedle patches	87:105	Microneedle patches are a promising source for transdermal diffusion of macromolecules and are designed to painlessly penetrate the skin.
35917312	6	64	theme	chitosan	996:1003	arg1	solution					1005:1012	the chitosan solution	992:1012	the chitosan solution	992:1012	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	11	65	theme	chitosan	1892:1899	arg1	patches					1927:1933	chitosan biodegradable microneedle patches	1892:1933	chitosan biodegradable microneedle patches	1892:1933	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	11	66	theme	commercial	2033:2042	arg1	manufacturing					2044:2056	commercial manufacturing	2033:2056	commercial manufacturing	2033:2056	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	2	67	theme	chitosan	256:263	arg1	patch					277:281	a biodegradable chitosan microneedle patch	240:281	a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle	240:330	In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested.
35917312	11	68	theme	positive	2012:2019	arg1	effects					2021:2027	positive effects	2012:2027	positive effects for commercial manufacturing	2012:2056	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	10	69	theme	abnormal	1778:1785	arg1	tissue					1787:1792	No abnormal tissue	1775:1792	No abnormal tissue	1775:1792	No abnormal tissue, swelling, or inflammation was observed in the implanted area.
35917312	6	70	theme	physical	877:884	arg1	characteristics					886:900	the physical characteristics	873:900	the physical characteristics	873:900	Results regarding the physical characteristics, chemical composition, and mechanical properties confirmed that rheological properties of the chitosan solution, present significant differences over time, demonstrating that reusing the solution on the fourth day results in failure patches.
35917312	2	71	theme	biodegradable	242:254	arg1	patch					277:281	a biodegradable chitosan microneedle patch	240:281	a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle	240:330	In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested.
35917312	4	72	theme	patch	717:721	arg1	analysis					693:700	compression testing analysis	673:700	compression testing analysis of microneedle patch	673:721	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	4	72	theme	patch	717:721	arg1	analysis					750:757	in-vitro drug release analysis	728:757	in-vitro drug release analysis	728:757	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	4	72	theme	patch	717:721	arg1	properties					661:670	in-vitro penetration mechanical properties	629:670	in-vitro penetration mechanical properties	629:670	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	11	73	theme	microneedle	1915:1925	arg1	patches					1927:1933	chitosan biodegradable microneedle patches	1892:1933	chitosan biodegradable microneedle patches	1892:1933	The results of this work show that chitosan biodegradable microneedle patches may be useful to deliver meloxicam to improve pain management of cattle with positive effects for commercial manufacturing.
35917312	9	74	theme	In-vivo	1575:1581	arg1	studies					1583:1589	In-vivo studies	1575:1589	In-vivo studies	1575:1589	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	2	75	dep	patch	277:281	arg1	deliver					286:292	deliver	286:292	to deliver meloxicam for managing pain in cattle	283:330	In this study, a biodegradable chitosan microneedle patch to deliver meloxicam for managing pain in cattle was tested.
35917312	4	76	theme	penetration	638:648	arg1	properties					661:670	in-vitro penetration mechanical properties	629:670	in-vitro penetration mechanical properties	629:670	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	9	77	theme	original	1730:1737	arg1	height					1739:1744	the original height	1726:1744	the original height of microneedle after 7 days	1726:1772	A full penetration of the microneedles into the skin can be obtained by applying approximately 3.2 N. In-vivo studies demonstrated that microneedle patches were capable of swelling and dissolving, exhibiting a dissolution percentage of more than 50% of the original height of microneedle after 7 days.
35917312	4	78	theme	in-vitro	629:636	arg1	properties					661:670	in-vitro penetration mechanical properties	629:670	in-vitro penetration mechanical properties	629:670	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
35917312	3	79	theme	preservation	512:523	arg1	optimization					419:430	optimization	419:430	optimization of fabrication	419:445	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	79	theme	preservation	512:523	arg1	analysis					500:507	analysis	500:507	analysis of preservation of the chemical composition after sterilization	500:571	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	79	theme	preservation	512:523	arg1	patches					410:416	the patches	406:416	the patches	406:416	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	3	79	theme	preservation	512:523	arg1	analysis					462:469	morphological analysis	448:469	morphological analysis of the microneedle patch	448:494	The potential of reuse of the polymeric solution to fabricate the patches, optimization of fabrication, morphological analysis of the microneedle patch and analysis of preservation of the chemical composition after sterilization were evaluated.
35917312	7	80	theme	patches	1364:1370	arg1	properties					1338:1347	the properties	1334:1347	the properties of microneedle patches	1334:1370	Morphological characteristics and chemical composition studies revealed that the process of sterilization (ethylene oxide gas) needed for implanting the patches into the skin did not affect the properties of microneedle patches.
35917312	4	81	theme	drug	737:740	arg1	release					742:748	in-vitro drug release	728:748	in-vitro drug release analysis	728:757	In-vitro analysis consisted of studying in-vitro penetration mechanical properties, compression testing analysis of microneedle patch, and in-vitro drug release analysis.
36549625	4	0	theme	Electron	844:851	arg1	SEM					865:867	SEM	865:867	SEM	865:867	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	4	0	theme	Electron	844:851	arg1	Microscopy					853:862	Scanning Electron Microscopy	835:862	The Scanning Electron Microscopy (SEM) images	831:875	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	8	1	dep	Salmonella	1510:1519	arg1	typhimurium					1521:1531	typhimurium	1521:1531	typhimurium	1521:1531	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	8	2	dep	Pseudomonas	1538:1548	arg1	fluorescens					1550:1560	fluorescens	1550:1560	fluorescens	1550:1560	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	8	3	theme	bacterial	1562:1570	arg1	strains					1572:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	2	4	theme	mechanical	476:485	arg1	properties					487:496	its better mechanical properties	465:496	its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %)	465:545	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	3	5	theme	WVP = 6.60 ± 0.31	685:701	arg1	permeability					671:682	less water vapor permeability	654:682	less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	654:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	5	theme	WVP = 6.60 ± 0.31	685:701	arg1	 × 10-8					711:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	4	6	theme	Scanning	835:842	arg1	SEM					865:867	SEM	865:867	SEM	865:867	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	4	6	theme	Scanning	835:842	arg1	Microscopy					853:862	Scanning Electron Microscopy	835:862	The Scanning Electron Microscopy (SEM) images	831:875	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	1	7	theme	current	178:184	arg1	investigation					186:198	the current investigation	174:198	the current investigation	174:198	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	6	8	theme	biopolymer	1201:1210	arg1	substrate					1212:1220	the biopolymer substrate	1197:1220	the biopolymer substrate	1197:1220	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	4	9	theme	agglomeration	907:919	arg1	phenomenon					893:902	the phenomenon	889:902	the phenomenon of agglomeration and point accumulation of REO	889:949	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	3	10	theme	best	790:793	arg1	properties					801:810	the best color properties	786:810	the best color properties among the samples	786:828	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	0	11	theme	carboxymethyl	100:112	arg1	cellulose					114:122	carboxymethyl cellulose	100:122	carboxymethyl cellulose	100:122	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	8	12	theme	antimicrobial	1435:1447	arg1	effects					1449:1455	antimicrobial effects	1435:1455	antimicrobial effects	1435:1455	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	4	13	theme	Microscopy	853:862	arg1	images					870:875	The Scanning Electron Microscopy (SEM) images	831:875	The Scanning Electron Microscopy (SEM) images	831:875	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	6	14	theme	spectroscopy	1116:1127	arg1	spectra					1136:1142	Fourier-transform infrared spectroscopy (FTIR) spectra	1089:1142	Fourier-transform infrared spectroscopy (FTIR) spectra	1089:1142	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	3	15	contain	had	650:652	arg2	properties					801:810	the best color properties	786:810	the best color properties among the samples	786:828	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	15	contain	had	650:652	arg1	it					647:648	it	647:648	it	647:648	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	15	contain	had	650:652	arg2	permeability					671:682	less water vapor permeability	654:682	less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	654:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	15	contain	had	650:652	arg2	 × 10-8					711:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	16	theme	color	795:799	arg1	properties					801:810	the best color properties	786:810	the best color properties among the samples	786:828	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	0	17	theme	rosemary	135:142	arg1	oil					154:156	rosemary essential oil	135:156	rosemary essential oil	135:156	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	2	18	theme	other	559:563	arg1	samples					576:582	other emulsified samples	559:582	other emulsified samples containing REO	559:597	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	7	19	theme	Adding	1326:1331	arg1	REO					1337:1339	Adding 5 % REO	1326:1339	Adding 5 % REO	1326:1339	Adding 5 % REO showed antioxidant effects.
36549625	6	20	theme	infrared	1107:1114	arg1	FTIR					1130:1133	FTIR	1130:1133	FTIR	1130:1133	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	6	20	theme	infrared	1107:1114	arg1	spectroscopy					1116:1127	Fourier-transform infrared spectroscopy	1089:1127	Fourier-transform infrared spectroscopy (FTIR) spectra	1089:1142	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	8	21	dep	Staphylococcus	1469:1482	arg1	aureus					1484:1489	aureus	1484:1489	aureus	1484:1489	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	1	22	theme	investigation	186:198	arg1	purpose					163:169	The purpose	159:169	The purpose of the current investigation	159:198	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	2	23	theme	due	458:460	arg1	REO					453:455	5 % REO	449:455	5 % REO	449:455	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	7	24	theme	antioxidant	1348:1358	arg1	effects					1360:1366	antioxidant effects	1348:1366	antioxidant effects	1348:1366	Adding 5 % REO showed antioxidant effects.
36549625	2	25	theme	better	469:474	arg1	properties					487:496	its better mechanical properties	465:496	its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %)	465:545	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	6	26	theme	Fourier-transform	1089:1105	arg1	FTIR					1130:1133	FTIR	1130:1133	FTIR	1130:1133	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	6	26	theme	Fourier-transform	1089:1105	arg1	spectroscopy					1116:1127	Fourier-transform infrared spectroscopy	1089:1127	Fourier-transform infrared spectroscopy (FTIR) spectra	1089:1142	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	8	27	contain	had	1431:1433	arg2	effects					1449:1455	antimicrobial effects	1435:1455	antimicrobial effects	1435:1455	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	8	27	contain	had	1431:1433	arg1	films					1410:1414	all films	1406:1414	all films containing REO	1406:1429	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	4	28	theme	point	925:929	arg1	accumulation					931:942	point accumulation	925:942	point accumulation of REO	925:949	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	7	29	theme	5 	1333:1334	arg1	REO					1337:1339	Adding 5 % REO	1326:1339	Adding 5 % REO	1326:1339	Adding 5 % REO showed antioxidant effects.
36549625	3	30	theme	control	737:743	arg1	sample					745:750	control sample	737:750	control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	737:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	30	theme	control	737:743	arg1	 × 10-8					773:779	8.21 ± 0.10 (g/mhPa) × 10-8	753:779	8.21 ± 0.10 (g/mhPa) × 10-8	753:779	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	8	31	theme	Salmonella	1510:1519	arg1	strains					1572:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	8	32	theme	Staphylococcus	1469:1482	arg1	strains					1572:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	3	33	theme	vapor	665:669	arg1	permeability					671:682	less water vapor permeability	654:682	less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	654:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	33	theme	vapor	665:669	arg1	 × 10-8					711:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	0	34	theme	Physico-chemical	0:15	arg1	characteristics					35:49	Physico-chemical and antimicrobial characteristics	0:49	Physico-chemical and antimicrobial characteristics of novel biodegradable films	0:78	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	1	35	theme	Carboxymethyl	283:295	arg1	biopolymers					313:323	Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers	266:323	biopolymers	313:323	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	2	36	theme	optimal	620:626	arg1	film					628:631	the optimal film	616:631	the optimal film	616:631	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	2	36	theme	optimal	620:626	arg1	film					433:436	The film	429:436	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO,	429:598	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	1	37	contain	containing	325:334	arg1	Gla					274:276	Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers	266:323	Gla	274:276	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	1	37	contain	containing	325:334	arg2	REO					361:363	REO	361:363	REO	361:363	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	1	37	contain	containing	325:334	arg2	oils					355:358	rosemary essential oils	336:358	rosemary essential oils (REO)	336:364	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	8	38	contain	containing	1416:1425	arg2	REO					1427:1429	REO	1427:1429	REO	1427:1429	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	8	38	contain	containing	1416:1425	arg1	films					1410:1414	all films	1406:1414	all films containing REO	1406:1429	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	2	39	theme	%	451:451	arg1	REO					453:455	5 % REO	449:455	5 % REO	449:455	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	1	40	theme	cellulose	297:305	arg1	biopolymers					313:323	Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers	266:323	biopolymers	313:323	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	2	41	theme	emulsified	565:574	arg1	samples					576:582	other emulsified samples	559:582	other emulsified samples containing REO	559:597	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	6	42	theme	Gla-CMC	1280:1286	arg1	chains					1288:1293	the Gla-CMC chains	1276:1293	the Gla-CMC chains	1276:1293	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	2	43	contain	containing	584:593	arg2	REO					595:597	REO	595:597	REO	595:597	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	2	43	contain	containing	584:593	arg1	samples					576:582	other emulsified samples	559:582	other emulsified samples containing REO	559:597	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	0	44	theme	antimicrobial	21:33	arg1	characteristics					35:49	Physico-chemical and antimicrobial characteristics	0:49	Physico-chemical and antimicrobial characteristics of novel biodegradable films	0:78	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	0	45	theme	essential	144:152	arg1	oil					154:156	rosemary essential oil	135:156	rosemary essential oil	135:156	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	1	46	theme	physicochemical	385:399	arg1	features					419:426	their physicochemical and antimicrobial features	379:426	their physicochemical and antimicrobial features	379:426	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	1	47	theme	novel	217:221	arg1	film					258:261	a novel functional composite biodegradable film	215:261	a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO)	215:364	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	0	48	theme	novel	54:58	arg1	films					74:78	novel biodegradable films	54:78	novel biodegradable films	54:78	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	1	49	theme	CMC	308:310	arg1	biopolymers					313:323	Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers	266:323	biopolymers	313:323	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	5	50	theme	film	1017:1020	arg1	compactness					998:1008	the increased compactness	984:1008	the increased compactness of the film in comparison to the film without the REO	984:1062	Also, 5 % of REO contributed to the increased compactness of the film in comparison to the film without the REO.
36549625	1	51	theme	functional	223:232	arg1	film					258:261	a novel functional composite biodegradable film	215:261	a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO)	215:364	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	3	52	theme	g/mhPa	766:771	arg1	sample					745:750	control sample	737:750	control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	737:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	52	theme	g/mhPa	766:771	arg1	 × 10-8					773:779	8.21 ± 0.10 (g/mhPa) × 10-8	753:779	8.21 ± 0.10 (g/mhPa) × 10-8	753:779	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	1	53	theme	antimicrobial	405:417	arg1	features					419:426	their physicochemical and antimicrobial features	379:426	their physicochemical and antimicrobial features	379:426	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	5	54	theme	REO	965:967	arg1	REO					965:967	REO	965:967	REO	965:967	Also, 5 % of REO contributed to the increased compactness of the film in comparison to the film without the REO.
36549625	5	54	theme	REO	965:967	arg1	%					960:960	5 %	958:960	5 % of REO	958:967	Also, 5 % of REO contributed to the increased compactness of the film in comparison to the film without the REO.
36549625	0	55	contain	containing	124:133	arg1	gellan					89:94	gellan	89:94	gellan	89:94	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	0	55	contain	containing	124:133	arg2	oil					154:156	rosemary essential oil	135:156	rosemary essential oil	135:156	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	1	56	theme	composite	234:242	arg1	film					258:261	a novel functional composite biodegradable film	215:261	a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO)	215:364	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	0	57	theme	films	74:78	arg1	characteristics					35:49	Physico-chemical and antimicrobial characteristics	0:49	Physico-chemical and antimicrobial characteristics of novel biodegradable films	0:78	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	8	58	theme	Escherichia	1492:1502	arg1	strains					1572:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	2	59	dep	properties	487:496	arg1	UTS = 13.44 ± 0.30 Mpa					499:520	UTS = 13.44 ± 0.30 Mpa	499:520	UTS = 13.44 ± 0.30 Mpa	499:520	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	2	59	dep	properties	487:496	arg1	%					544:544	SB = 21.14 ± 1.15 %	526:544	SB = 21.14 ± 1.15 %	526:544	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	8	60	dep	Escherichia	1492:1502	arg1	coli					1504:1507	coli	1504:1507	coli	1504:1507	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	6	61	theme	new	1148:1150	arg1	bonds					1161:1165	no new chemical bonds	1145:1165	no new chemical bonds	1145:1165	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	0	62	theme	biodegradable	60:72	arg1	films					74:78	novel biodegradable films	54:78	novel biodegradable films	54:78	Physico-chemical and antimicrobial characteristics of novel biodegradable films based on gellan and carboxymethyl cellulose containing rosemary essential oil.
36549625	3	63	theme	8.21 ± 0.10	753:763	arg1	sample					745:750	control sample	737:750	control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	737:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	63	theme	8.21 ± 0.10	753:763	arg1	 × 10-8					773:779	8.21 ± 0.10 (g/mhPa) × 10-8	753:779	8.21 ± 0.10 (g/mhPa) × 10-8	753:779	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	64	theme	g/mhPa	704:709	arg1	permeability					671:682	less water vapor permeability	654:682	less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	654:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	64	theme	g/mhPa	704:709	arg1	 × 10-8					711:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	8	65	theme	antimicrobial	1385:1397	arg1	tests					1399:1403	the antimicrobial tests	1381:1403	the antimicrobial tests	1381:1403	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	1	66	theme	rosemary	336:343	arg1	REO					361:363	REO	361:363	REO	361:363	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	1	66	theme	rosemary	336:343	arg1	oils					355:358	rosemary essential oils	336:358	rosemary essential oils (REO)	336:364	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	5	67	theme	increased	988:996	arg1	compactness					998:1008	the increased compactness	984:1008	the increased compactness of the film in comparison to the film without the REO	984:1062	Also, 5 % of REO contributed to the increased compactness of the film in comparison to the film without the REO.
36549625	6	68	theme	chemical	1152:1159	arg1	bonds					1161:1165	no new chemical bonds	1145:1165	no new chemical bonds	1145:1165	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	1	69	theme	biodegradable	244:256	arg1	film					258:261	a novel functional composite biodegradable film	215:261	a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO)	215:364	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	7	70	theme	%	1335:1335	arg1	REO					1337:1339	Adding 5 % REO	1326:1339	Adding 5 % REO	1326:1339	Adding 5 % REO showed antioxidant effects.
36549625	3	71	theme	water	659:663	arg1	permeability					671:682	less water vapor permeability	654:682	less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8)	654:780	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	3	71	theme	water	659:663	arg1	 × 10-8					711:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	WVP = 6.60 ± 0.31 (g/mhPa) × 10-8	685:717	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	6	72	theme	spectra	1136:1142	arg1	results					1078:1084	the results	1074:1084	the results of Fourier-transform infrared spectroscopy (FTIR) spectra	1074:1142	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	4	73	theme	REO	947:949	arg1	agglomeration					907:919	agglomeration	907:919	agglomeration	907:919	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	4	73	theme	REO	947:949	arg1	accumulation					931:942	point accumulation	925:942	point accumulation of REO	925:949	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	5	74	from	compactness	998:1008	arg1	comparison					1025:1034	comparison	1025:1034	comparison to the film without the REO	1025:1062	Also, 5 % of REO contributed to the increased compactness of the film in comparison to the film without the REO.
36549625	2	75	contain	containing	438:447	arg1	film					628:631	the optimal film	616:631	the optimal film	616:631	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	2	75	contain	containing	438:447	arg1	film					433:436	The film	429:436	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO,	429:598	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	2	75	contain	containing	438:447	arg2	REO					453:455	5 % REO	449:455	5 % REO	449:455	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	6	76	theme	film	1310:1313	arg1	substrate					1315:1323	the film substrate	1306:1323	the film substrate	1306:1323	Based on the results of Fourier-transform infrared spectroscopy (FTIR) spectra, no new chemical bonds were created by adding REO to the biopolymer substrate, and the REO was well dispersed and distributed among the Gla-CMC chains throughout the film substrate.
36549625	3	77	dep	sample	745:750	arg1	comparison					723:732	comparison	723:732	comparison	723:732	Furthermore, it had less water vapor permeability (WVP = 6.60 ± 0.31 (g/mhPa) × 10-8) in comparison to control sample (8.21 ± 0.10 (g/mhPa) × 10-8) and the best color properties among the samples.
36549625	8	78	theme	Pseudomonas	1538:1548	arg1	strains					1572:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains	1465:1578	Considering the antimicrobial tests, all films containing REO had antimicrobial effects against the Staphylococcus aureus, Escherichia coli, Salmonella typhimurium, and Pseudomonas fluorescens bacterial strains.
36549625	4	79	theme	accumulation	931:942	arg1	phenomenon					893:902	the phenomenon	889:902	the phenomenon of agglomeration and point accumulation of REO	889:949	The Scanning Electron Microscopy (SEM) images didn't show the phenomenon of agglomeration and point accumulation of REO.
36549625	1	80	theme	essential	345:353	arg1	REO					361:363	REO	361:363	REO	361:363	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	1	80	theme	essential	345:353	arg1	oils					355:358	rosemary essential oils	336:358	rosemary essential oils (REO)	336:364	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
36549625	2	81	theme	5 	449:450	arg1	REO					453:455	5 % REO	449:455	5 % REO	449:455	The film containing 5 % REO, due to its better mechanical properties (UTS = 13.44 ± 0.30 Mpa and SB = 21.14 ± 1.15 %) compared to other emulsified samples containing REO, was selected as the optimal film.
36549625	1	82	theme	Gellan	266:271	arg1	Gla					274:276	Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers	266:323	Gla	274:276	The purpose of the current investigation was to produce a novel functional composite biodegradable film by Gellan (Gla) and Carboxymethyl cellulose (CMC) biopolymers containing rosemary essential oils (REO) and evaluate their physicochemical and antimicrobial features.
35832119	6	0	theme	glutathione	896:906	arg1	GSH-Px					920:925	GSH-Px	920:925	GSH-Px	920:925	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	6	0	theme	glutathione	896:906	arg1	peroxidase					908:917	glutathione peroxidase	896:917	glutathione peroxidase (GSH-Px) activity	896:935	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	2	1	theme	biomedical	374:383	arg1	field					385:389	the biomedical field	370:389	the biomedical field	370:389	Selenium nanoparticles (SeNPs) have attracted increasing attention in the biomedical field, given their antiviral effects.
35832119	1	2	theme	global	277:282	arg1	industry					290:297	the global swine industry	273:297	the global swine industry	273:297	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	8	3	theme	ROS	1118:1120	arg1	generation					1122:1131	ROS generation	1118:1131	ROS generation	1118:1131	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	10	4	theme	N	1573:1573	arg1	protein					1575:1581	N protein	1573:1581	N protein	1573:1581	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	1	5	theme	prevalent	193:201	arg1	virus					167:171	Introduction Porcine reproductive and respiratory syndrome virus	108:171	virus	167:171	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	5	theme	prevalent	193:201	arg1	pathogen					221:228	a highly prevalent and endemic swine pathogen	184:228	a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry	184:297	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	5	theme	prevalent	193:201	arg1	reproductive					129:140	reproductive	129:140	reproductive	129:140	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	6	6	theme	glutathione	873:883	arg1	concentrations					826:839	the concentrations	822:839	the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH)	822:889	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	6	6	theme	glutathione	873:883	arg1	activity					928:935	glutathione peroxidase (GSH-Px) activity	896:935	glutathione peroxidase (GSH-Px) activity	896:935	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	8	7	theme	dihydroethidium	1215:1229	arg1	staining					1231:1238	dihydroethidium staining	1215:1238	dihydroethidium staining	1215:1238	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	12	8	dep	Conclusion	1892:1901	arg1	demonstrated					1917:1928	demonstrated	1917:1928	demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications	1917:2189	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	1	9	theme	endemic	207:213	arg1	virus					167:171	Introduction Porcine reproductive and respiratory syndrome virus	108:171	virus	167:171	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	9	theme	endemic	207:213	arg1	pathogen					221:228	a highly prevalent and endemic swine pathogen	184:228	a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry	184:297	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	9	theme	endemic	207:213	arg1	reproductive					129:140	reproductive	129:140	reproductive	129:140	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	11	10	theme	ROS	1795:1797	arg1	generation					1799:1808	ROS generation	1795:1808	ROS generation	1795:1808	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	8	11	theme	/caspase-3/PARP	1159:1173	arg1	expression					1183:1192	JNK /caspase-3/PARP protein expression	1155:1192	JNK /caspase-3/PARP protein expression	1155:1192	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	12	12	theme	further	2143:2149	arg1	investigation					2151:2163	further investigation	2143:2163	further investigation for clinical applications	2143:2189	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	10	13	theme	hours	1612:1616	arg1	hpi					1634:1636	hpi	1634:1636	hpi	1634:1636	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	10	13	theme	hours	1612:1616	arg1	post-infection					1618:1631	24 and 48 hours post-infection	1602:1631	24 and 48 hours post-infection (hpi)	1602:1637	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	9	14	theme	H2O2	1474:1477	arg1	synthesis					1479:1487	H2O2 synthesis	1474:1487	H2O2 synthesis	1474:1487	Results The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis.
35832119	12	15	theme	signaling	2011:2019	arg1	pathway					2021:2027	the ROS/JNK signaling pathway	1999:2027	the ROS/JNK signaling pathway	1999:2027	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	5	16	theme	Marc-145	713:720	arg1	cells					722:726	Marc-145 cells	713:726	Marc-145 cells	713:726	Marc-145 cells were infected with r-PRRSV-EGFP (0.1 MOI) and inoculated with CS-SeNPs (10 μM).
35832119	12	17	theme	antiviral	2098:2106	arg1	activity					2108:2115	the potential antiviral activity	2084:2115	the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications	2084:2189	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	11	18	theme	phosphorylation	1815:1829	arg1	levels					1831:1836	JNK phosphorylation levels	1811:1836	JNK phosphorylation levels	1811:1836	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	7	19	theme	ORF5	989:992	arg1	expression					998:1007	ORF5 RNA expression	989:1007	ORF5 RNA expression	989:1007	ORF5 RNA expression, viral titer, and nucleocapsid (N) protein expression were assessed using qRT-PCR, TCID50, and Western blot.
35832119	1	20	theme	syndrome	158:165	arg1	virus					167:171	Introduction Porcine reproductive and respiratory syndrome virus	108:171	virus	167:171	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	20	theme	syndrome	158:165	arg1	pathogen					221:228	a highly prevalent and endemic swine pathogen	184:228	a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry	184:297	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	20	theme	syndrome	158:165	arg1	reproductive					129:140	reproductive	129:140	reproductive	129:140	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	21	theme	swine	284:288	arg1	industry					290:297	the global swine industry	273:297	the global swine industry	273:297	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	11	22	from	increase	1662:1669	arg1	rates					1684:1688	apoptosis rates	1674:1688	apoptosis rates	1674:1688	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	11	23	theme	CS-SeNPs	1755:1762	arg1	inoculation					1764:1774	CS-SeNPs inoculation	1755:1774	CS-SeNPs inoculation	1755:1774	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	6	24	theme	commercial	966:975	arg1	kits					983:986	specific commercial assay kits	957:986	specific commercial assay kits	957:986	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	3	25	theme	inhibitory	459:468	arg1	effect					470:475	the inhibitory effect	455:475	the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication	455:532	This study aimed to investigate the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication.
35832119	2	26	theme	antiviral	404:412	arg1	effects					414:420	their antiviral effects	398:420	their antiviral effects	398:420	Selenium nanoparticles (SeNPs) have attracted increasing attention in the biomedical field, given their antiviral effects.
35832119	0	27	theme	Chitosan-Coated	0:14	arg1	Nanoparticles					25:37	Chitosan-Coated Selenium Nanoparticles	0:37	Chitosan-Coated Selenium Nanoparticles	0:37	Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro.
35832119	3	28	theme	SeNPs	496:500	arg1	effect					470:475	the inhibitory effect	455:475	the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication	455:532	This study aimed to investigate the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication.
35832119	9	29	theme	CS-SeNPs	1307:1314	arg1	treatment					1316:1324	CS-SeNPs treatment	1307:1324	CS-SeNPs treatment	1307:1324	Results The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis.
35832119	0	30	theme	PRRSV	49:53	arg1	Replication					55:65	PRRSV Replication	49:65	PRRSV Replication	49:65	Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro.
35832119	10	31	theme	gene	1538:1541	arg1	expression					1543:1552	ORF5 gene expression	1533:1552	ORF5 gene expression	1533:1552	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	11	32	theme	caspase-3	1855:1863	arg1	levels					1831:1836	JNK phosphorylation levels	1811:1836	JNK phosphorylation levels	1811:1836	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	11	32	theme	caspase-3	1855:1863	arg1	cleavage					1843:1850	cleavage	1843:1850	cleavage of caspase-3 and PARP	1843:1872	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	11	32	theme	caspase-3	1855:1863	arg1	generation					1799:1808	ROS generation	1795:1808	ROS generation	1795:1808	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	9	33	theme	oxidative	1351:1359	arg1	stress					1361:1366	oxidative stress	1351:1366	oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis	1351:1487	Results The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis.
35832119	6	34	theme	peroxide	853:860	arg1	concentrations					826:839	the concentrations	822:839	the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH)	822:889	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	6	34	theme	peroxide	853:860	arg1	activity					928:935	glutathione peroxidase (GSH-Px) activity	896:935	glutathione peroxidase (GSH-Px) activity	896:935	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	9	35	dep	Results	1275:1281	arg1	showed					1295:1300	showed	1295:1300	showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis	1295:1487	Results The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis.
35832119	12	36	dep	inhibiting	2038:2047	arg1	suggested					2074:2082	suggested	2074:2082	suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications	2074:2189	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	1	37	theme	significant	242:252	arg1	losses					263:268	significant economic losses	242:268	significant economic losses	242:268	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	10	38	theme	Marc-145	1642:1649	arg1	cells					1651:1655	Marc-145 cells	1642:1655	Marc-145 cells	1642:1655	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	9	39	theme	r-PRRSV-EGFP	1379:1390	arg1	infection					1392:1400	r-PRRSV-EGFP infection	1379:1400	r-PRRSV-EGFP infection	1379:1400	Results The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis.
35832119	6	40	theme	peroxidase	908:917	arg1	activity					928:935	glutathione peroxidase (GSH-Px) activity	896:935	glutathione peroxidase (GSH-Px) activity	896:935	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	3	41	from	effect	470:475	arg1	replication					522:532	PRRSV replication	516:532	PRRSV replication	516:532	This study aimed to investigate the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication.
35832119	11	42	theme	PARP	1869:1872	arg1	levels					1831:1836	JNK phosphorylation levels	1811:1836	JNK phosphorylation levels	1811:1836	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	11	42	theme	PARP	1869:1872	arg1	cleavage					1843:1850	cleavage	1843:1850	cleavage of caspase-3 and PARP	1843:1872	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	11	42	theme	PARP	1869:1872	arg1	generation					1799:1808	ROS generation	1795:1808	ROS generation	1795:1808	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	12	43	theme	PRRSV	2049:2053	arg1	replication					2055:2065	PRRSV replication	2049:2065	PRRSV replication	2049:2065	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	2	44	from	attention	357:365	arg1	field					385:389	the biomedical field	370:389	the biomedical field	370:389	Selenium nanoparticles (SeNPs) have attracted increasing attention in the biomedical field, given their antiviral effects.
35832119	9	45	theme	GSH-Px	1416:1421	arg1	activity					1423:1430	GSH-Px activity	1416:1430	GSH-Px activity	1416:1430	Results The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis.
35832119	12	46	theme	ROS/JNK	2003:2009	arg1	pathway					2021:2027	the ROS/JNK signaling pathway	1999:2027	the ROS/JNK signaling pathway	1999:2027	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	2	47	theme	Selenium	300:307	arg1	nanoparticles					309:321	Selenium nanoparticles	300:321	Selenium nanoparticles (SeNPs)	300:329	Selenium nanoparticles (SeNPs) have attracted increasing attention in the biomedical field, given their antiviral effects.
35832119	2	47	theme	Selenium	300:307	arg1	SeNPs					324:328	SeNPs	324:328	SeNPs	324:328	Selenium nanoparticles (SeNPs) have attracted increasing attention in the biomedical field, given their antiviral effects.
35832119	4	48	theme	element	692:698	arg1	composition					700:710	element composition	692:710	element composition	692:710	Methods In this study, CS-SeNPs were synthesized by chemical reduction and characterized by assessing the morphology, size distribution, zeta potential, and element composition.
35832119	12	49	theme	Marc-145	1980:1987	arg1	cells					1989:1993	Marc-145 cells	1980:1993	Marc-145 cells	1980:1993	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	7	50	theme	protein	1044:1050	arg1	expression					1052:1061	nucleocapsid (N) protein expression	1027:1061	nucleocapsid (N) protein expression	1027:1061	ORF5 RNA expression, viral titer, and nucleocapsid (N) protein expression were assessed using qRT-PCR, TCID50, and Western blot.
35832119	9	51	theme	GSH	1443:1445	arg1	production					1447:1456	GSH production	1443:1456	GSH production	1443:1456	Results The results showed that CS-SeNPs treatment significantly suppressed oxidative stress induced by r-PRRSV-EGFP infection by increasing GSH-Px activity, promoting GSH production, and inhibiting H2O2 synthesis.
35832119	8	52	theme	apoptosis	1134:1142	arg1	rates					1144:1148	apoptosis rates	1134:1148	apoptosis rates	1134:1148	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	6	53	theme	specific	957:964	arg1	kits					983:986	specific commercial assay kits	957:986	specific commercial assay kits	957:986	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	12	54	theme	clinical	2169:2176	arg1	applications					2178:2189	clinical applications	2169:2189	clinical applications	2169:2189	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	10	55	theme	r-PRRSV-EGFP	1586:1597	arg1	expression					1543:1552	ORF5 gene expression	1533:1552	ORF5 gene expression	1533:1552	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	10	55	theme	r-PRRSV-EGFP	1586:1597	arg1	titers					1561:1566	viral titers	1555:1566	viral titers	1555:1566	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	10	55	theme	r-PRRSV-EGFP	1586:1597	arg1	protein					1575:1581	N protein	1573:1581	N protein	1573:1581	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	11	56	theme	JNK	1811:1813	arg1	levels					1831:1836	JNK phosphorylation levels	1811:1836	JNK phosphorylation levels	1811:1836	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	1	57	theme	swine	215:219	arg1	virus					167:171	Introduction Porcine reproductive and respiratory syndrome virus	108:171	virus	167:171	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	57	theme	swine	215:219	arg1	pathogen					221:228	a highly prevalent and endemic swine pathogen	184:228	a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry	184:297	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	57	theme	swine	215:219	arg1	reproductive					129:140	reproductive	129:140	reproductive	129:140	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	12	58	theme	CS-SeNPs	2120:2127	arg1	activity					2108:2115	the potential antiviral activity	2084:2115	the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications	2084:2189	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	7	59	theme	nucleocapsid	1027:1038	arg1	expression					1052:1061	nucleocapsid (N) protein expression	1027:1061	nucleocapsid (N) protein expression	1027:1061	ORF5 RNA expression, viral titer, and nucleocapsid (N) protein expression were assessed using qRT-PCR, TCID50, and Western blot.
35832119	8	60	theme	JNK	1155:1157	arg1	expression					1183:1192	JNK /caspase-3/PARP protein expression	1155:1192	JNK /caspase-3/PARP protein expression	1155:1192	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	8	61	theme	flow	1241:1244	arg1	cytometry					1246:1254	flow cytometry	1241:1254	flow cytometry	1241:1254	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	1	62	theme	respiratory	146:156	arg1	virus					167:171	Introduction Porcine reproductive and respiratory syndrome virus	108:171	virus	167:171	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	62	theme	respiratory	146:156	arg1	pathogen					221:228	a highly prevalent and endemic swine pathogen	184:228	a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry	184:297	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	1	62	theme	respiratory	146:156	arg1	reproductive					129:140	reproductive	129:140	reproductive	129:140	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	8	63	theme	protein	1175:1181	arg1	expression					1183:1192	JNK /caspase-3/PARP protein expression	1155:1192	JNK /caspase-3/PARP protein expression	1155:1192	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	12	64	from	apoptosis	1967:1975	arg1	cells					1989:1993	Marc-145 cells	1980:1993	Marc-145 cells	1980:1993	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	12	65	theme	potential	2088:2096	arg1	activity					2108:2115	the potential antiviral activity	2084:2115	the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications	2084:2189	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	7	66	theme	viral	1010:1014	arg1	titer					1016:1020	viral titer	1010:1020	viral titer	1010:1020	ORF5 RNA expression, viral titer, and nucleocapsid (N) protein expression were assessed using qRT-PCR, TCID50, and Western blot.
35832119	7	67	theme	RNA	994:996	arg1	expression					998:1007	ORF5 RNA expression	989:1007	ORF5 RNA expression	989:1007	ORF5 RNA expression, viral titer, and nucleocapsid (N) protein expression were assessed using qRT-PCR, TCID50, and Western blot.
35832119	8	68	theme	Western	1261:1267	arg1	blot					1269:1272	Western blot	1261:1272	Western blot	1261:1272	ROS generation, apoptosis rates, and JNK /caspase-3/PARP protein expression were evaluated using dihydroethidium staining, flow cytometry, and Western blot.
35832119	6	69	theme	assay	977:981	arg1	kits					983:986	specific commercial assay kits	957:986	specific commercial assay kits	957:986	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	4	70	theme	size	653:656	arg1	distribution					658:669	size distribution	653:669	size distribution	653:669	Methods In this study, CS-SeNPs were synthesized by chemical reduction and characterized by assessing the morphology, size distribution, zeta potential, and element composition.
35832119	12	71	theme	PRRSV-induced	1953:1965	arg1	apoptosis					1967:1975	PRRSV-induced apoptosis	1953:1975	PRRSV-induced apoptosis in Marc-145 cells	1953:1993	Conclusion These results demonstrated that CS-SeNPs suppress PRRSV-induced apoptosis in Marc-145 cells via the ROS/JNK signaling pathway, thereby inhibiting PRRSV replication, which suggested the potential antiviral activity of CS-SeNPs that deserves further investigation for clinical applications.
35832119	0	72	theme	Selenium	16:23	arg1	Nanoparticles					25:37	Chitosan-Coated Selenium Nanoparticles	0:37	Chitosan-Coated Selenium Nanoparticles	0:37	Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro.
35832119	4	73	theme	chemical	587:594	arg1	reduction					596:604	chemical reduction	587:604	chemical reduction	587:604	Methods In this study, CS-SeNPs were synthesized by chemical reduction and characterized by assessing the morphology, size distribution, zeta potential, and element composition.
35832119	10	74	theme	CS-SeNPs	1490:1497	arg1	treatment					1499:1507	CS-SeNPs treatment	1490:1507	CS-SeNPs treatment	1490:1507	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	11	75	theme	apoptosis	1674:1682	arg1	rates					1684:1688	apoptosis rates	1674:1688	apoptosis rates	1674:1688	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	7	76	theme	Western	1104:1110	arg1	blot					1112:1115	Western blot	1104:1115	Western blot	1104:1115	ORF5 RNA expression, viral titer, and nucleocapsid (N) protein expression were assessed using qRT-PCR, TCID50, and Western blot.
35832119	4	77	theme	zeta	672:675	arg1	potential					677:685	zeta potential	672:685	zeta potential	672:685	Methods In this study, CS-SeNPs were synthesized by chemical reduction and characterized by assessing the morphology, size distribution, zeta potential, and element composition.
35832119	3	78	theme	chitosan-coated	480:494	arg1	CS-SeNPs					503:510	CS-SeNPs	503:510	CS-SeNPs	503:510	This study aimed to investigate the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication.
35832119	3	78	theme	chitosan-coated	480:494	arg1	SeNPs					496:500	chitosan-coated SeNPs	480:500	chitosan-coated SeNPs (CS-SeNPs)	480:511	This study aimed to investigate the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication.
35832119	0	79	theme	ROS/JNK-Mediated	71:86	arg1	Apoptosis					88:96	ROS/JNK-Mediated Apoptosis	71:96	ROS/JNK-Mediated Apoptosis	71:96	Chitosan-Coated Selenium Nanoparticles Attenuate PRRSV Replication and ROS/JNK-Mediated Apoptosis in vitro.
35832119	4	80	dep	Methods	535:541	arg1	characterized					610:622	characterized	610:622	characterized by assessing the morphology, size distribution, zeta potential, and element composition	610:710	Methods In this study, CS-SeNPs were synthesized by chemical reduction and characterized by assessing the morphology, size distribution, zeta potential, and element composition.
35832119	4	80	dep	Methods	535:541	arg1	synthesized					572:582	synthesized	572:582	synthesized by chemical reduction	572:604	Methods In this study, CS-SeNPs were synthesized by chemical reduction and characterized by assessing the morphology, size distribution, zeta potential, and element composition.
35832119	10	81	theme	ORF5	1533:1536	arg1	expression					1543:1552	ORF5 gene expression	1533:1552	ORF5 gene expression	1533:1552	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35832119	11	82	theme	r-PRRSV-EGFP	1701:1712	arg1	infection					1714:1722	r-PRRSV-EGFP infection	1701:1722	r-PRRSV-EGFP infection	1701:1722	The increase in apoptosis rates induced by r-PRRSV-EGFP infection was significantly decreased by CS-SeNPs inoculation through inhibiting ROS generation, JNK phosphorylation levels, and cleavage of caspase-3 and PARP mainly at 48 hpi.
35832119	3	83	theme	PRRSV	516:520	arg1	replication					522:532	PRRSV replication	516:532	PRRSV replication	516:532	This study aimed to investigate the inhibitory effect of chitosan-coated SeNPs (CS-SeNPs) on PRRSV replication.
35832119	6	84	theme	hydrogen	844:851	arg1	H2O2					863:866	H2O2	863:866	H2O2	863:866	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	6	84	theme	hydrogen	844:851	arg1	peroxide					853:860	hydrogen peroxide	844:860	hydrogen peroxide (H2O2)	844:867	Subsequently, the concentrations of hydrogen peroxide (H2O2) and glutathione (GSH), and glutathione peroxidase (GSH-Px) activity were measured using specific commercial assay kits.
35832119	2	85	theme	increasing	346:355	arg1	attention					357:365	increasing attention	346:365	increasing attention in the biomedical field	346:389	Selenium nanoparticles (SeNPs) have attracted increasing attention in the biomedical field, given their antiviral effects.
35832119	1	86	theme	economic	254:261	arg1	losses					263:268	significant economic losses	242:268	significant economic losses	242:268	Introduction Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly prevalent and endemic swine pathogen that causes significant economic losses to the global swine industry.
35832119	10	87	theme	viral	1555:1559	arg1	titers					1561:1566	viral titers	1555:1566	viral titers	1555:1566	CS-SeNPs treatment significantly inhibited ORF5 gene expression, viral titers, and N protein of r-PRRSV-EGFP at 24 and 48 hours post-infection (hpi) in Marc-145 cells.
35913239	2	0	theme	silver	523:528	arg1	nanoparticles					530:542	silver nanoparticles	523:542	silver nanoparticles (AgNPs) of around 30 nm in diameter	523:578	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	2	0	theme	silver	523:528	arg1	AgNPs					545:549	AgNPs	545:549	AgNPs	545:549	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	3	1	theme	chitosan/Eudragit	727:743	arg1	coating					749:755	a pH-sensitive chitosan/Eudragit 100 coating	712:755	a pH-sensitive chitosan/Eudragit 100 coating	712:755	To ensure a controlled release of the bactericidal substance, the samples were additionally coated with a pH-sensitive chitosan/Eudragit 100 coating, also exhibiting bactericidal properties.
35913239	1	2	theme	titanium	184:191	arg1	samples					193:199	the titanium samples	180:199	the titanium samples	180:199	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	4	3	theme	XPS	880:882	arg1	techniques					884:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques.
35913239	5	4	theme	corrosion	913:921	arg1	properties					923:932	corrosion properties	913:932	corrosion properties	913:932	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	2	5	theme	silver	410:415	arg1	film					422:425	silver 5 nm film	410:425	silver 5 nm film	410:425	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	0	6	theme	chitosan/Eudragit	115:131	arg1	polymers					133:140	chitosan/Eudragit polymers	115:140	chitosan/Eudragit polymers	115:140	Antibacterial properties of laser-encapsulated titanium oxide nanotubes decorated with nanosilver and covered with chitosan/Eudragit polymers.
35913239	4	7	theme	modified	803:810	arg1	samples					821:827	The modified titanium samples	799:827	The modified titanium samples	799:827	The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques.
35913239	1	8	theme	titanium	320:327	arg1	surface					347:353	a titanium oxide nanotubular surface	318:353	a titanium oxide nanotubular surface	318:353	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	7	9	from	increase	1212:1219	arg1	silver					1238:1243	silver	1238:1243	silver	1238:1243	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	7	9	from	increase	1212:1219	arg1	amount					1228:1233	the amount	1224:1233	the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH	1224:1332	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	2	10	theme	5 nm	417:420	arg1	film					422:425	silver 5 nm film	410:425	silver 5 nm film	410:425	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	1	11	theme	oxide	329:333	arg1	surface					347:353	a titanium oxide nanotubular surface	318:353	a titanium oxide nanotubular surface	318:353	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	4	12	theme	Raman	869:873	arg1	techniques					884:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques.
35913239	7	13	theme	avoiding	1425:1432	arg1	effect					1440:1445	exposure avoiding burst effect	1416:1445	exposure avoiding burst effect	1416:1445	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	7	14	with	samples	1360:1366	arg1	biopolymer					1377:1386	the biopolymer	1373:1386	the biopolymer coating after three days of exposure avoiding burst effect	1373:1445	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	2	15	from	appearance	509:518	arg1	surface					599:605	such a modified surface	583:605	such a modified surface	583:605	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	2	16	from	30 nm	562:566	arg1	diameter					571:578	diameter	571:578	diameter	571:578	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	5	17	theme	coating	951:957	arg1	adhesion					935:942	adhesion	935:942	adhesion of the coating to the substrate	935:974	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	17	theme	coating	951:957	arg1	wettability					900:910	The wettability	896:910	The wettability	896:910	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	17	theme	coating	951:957	arg1	properties					923:932	corrosion properties	913:932	corrosion properties	913:932	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	17	theme	coating	951:957	arg1	release					981:987	the release	977:987	the release of AgNPs into solutions simulating body fluids at different pH	977:1050	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	17	theme	coating	951:957	arg1	properties					1071:1080	antibacterial properties	1057:1080	antibacterial properties	1057:1080	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	7	18	theme	acidic	1324:1329	arg1	pH					1331:1332	acidic pH	1324:1332	acidic pH	1324:1332	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	0	19	theme	Antibacterial	0:12	arg1	properties					14:23	Antibacterial properties	0:23	Antibacterial properties of laser-encapsulated titanium oxide nanotubes	0:70	Antibacterial properties of laser-encapsulated titanium oxide nanotubes decorated with nanosilver and covered with chitosan/Eudragit polymers.
35913239	1	20	theme	nanotubular	335:345	arg1	surface					347:353	a titanium oxide nanotubular surface	318:353	a titanium oxide nanotubular surface	318:353	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	7	21	theme	exposure	1416:1423	arg1	effect					1440:1445	exposure avoiding burst effect	1416:1445	exposure avoiding burst effect	1416:1445	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	1	22	theme	electrochemical	219:233	arg1	oxidation					235:243	electrochemical oxidation	219:243	electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface	219:353	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	0	23	theme	laser-encapsulated	28:45	arg1	nanotubes					62:70	laser-encapsulated titanium oxide nanotubes	28:70	laser-encapsulated titanium oxide nanotubes	28:70	Antibacterial properties of laser-encapsulated titanium oxide nanotubes decorated with nanosilver and covered with chitosan/Eudragit polymers.
35913239	4	24	theme	SEM	854:856	arg1	techniques					884:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques.
35913239	5	25	theme	body	1024:1027	arg1	fluids					1029:1034	body fluids	1024:1034	body fluids	1024:1034	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	7	26	theme	fluid	1306:1310	arg1	solution					1312:1319	a simulated body fluid solution	1289:1319	a simulated body fluid solution at acidic pH	1289:1332	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	8	27	theme	proposed	1452:1459	arg1	modification					1461:1472	The proposed modification	1448:1472	The proposed modification	1448:1472	The proposed modification was effective against both Gram-positive and Gram-negative bacteria.
35913239	8	27	theme	proposed	1452:1459	arg1	effective					1478:1486	effective	1478:1486	effective	1478:1486	The proposed modification was effective against both Gram-positive and Gram-negative bacteria.
35913239	0	28	theme	oxide	56:60	arg1	nanotubes					62:70	laser-encapsulated titanium oxide nanotubes	28:70	laser-encapsulated titanium oxide nanotubes	28:70	Antibacterial properties of laser-encapsulated titanium oxide nanotubes decorated with nanosilver and covered with chitosan/Eudragit polymers.
35913239	3	29	theme	bactericidal	774:785	arg1	properties					787:796	bactericidal properties	774:796	bactericidal properties	774:796	To ensure a controlled release of the bactericidal substance, the samples were additionally coated with a pH-sensitive chitosan/Eudragit 100 coating, also exhibiting bactericidal properties.
35913239	7	30	theme	body	1301:1304	arg1	solution					1312:1319	a simulated body fluid solution	1289:1319	a simulated body fluid solution at acidic pH	1289:1332	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	4	31	theme	EDS	859:861	arg1	techniques					884:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques.
35913239	2	32	theme	nanotubes	448:456	arg1	tops					436:439	the tops	432:439	the tops of the nanotubes	432:456	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	0	33	theme	titanium	47:54	arg1	nanotubes					62:70	laser-encapsulated titanium oxide nanotubes	28:70	laser-encapsulated titanium oxide nanotubes	28:70	Antibacterial properties of laser-encapsulated titanium oxide nanotubes decorated with nanosilver and covered with chitosan/Eudragit polymers.
35913239	2	34	theme	30 nm	562:566	arg1	nanoparticles					530:542	silver nanoparticles	523:542	silver nanoparticles (AgNPs) of around 30 nm in diameter	523:578	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	2	34	theme	30 nm	562:566	arg1	AgNPs					545:549	AgNPs	545:549	AgNPs	545:549	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	3	35	theme	bactericidal	646:657	arg1	substance					659:667	the bactericidal substance	642:667	the bactericidal substance	642:667	To ensure a controlled release of the bactericidal substance, the samples were additionally coated with a pH-sensitive chitosan/Eudragit 100 coating, also exhibiting bactericidal properties.
35913239	2	36	theme	laser	476:480	arg1	treatment					482:490	laser treatment	476:490	laser treatment	476:490	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	1	37	theme	fluoride-containing	252:270	arg1	electrolyte					296:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	2	38	theme	around	555:560	arg1	30 nm					562:566	around 30 nm	555:566	around 30 nm in diameter	555:578	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	2	39	from	nanoparticles	530:542	arg1	diameter					571:578	diameter	571:578	diameter	571:578	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	4	40	theme	AFM	864:866	arg1	techniques					884:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	SEM, EDS, AFM, Raman, and XPS techniques	854:893	The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques.
35913239	8	41	theme	Gram-negative	1519:1531	arg1	bacteria					1533:1540	both Gram-positive and Gram-negative bacteria	1496:1540	both Gram-positive and Gram-negative bacteria	1496:1540	The proposed modification was effective against both Gram-positive and Gram-negative bacteria.
35913239	0	42	theme	nanotubes	62:70	arg1	properties					14:23	Antibacterial properties	0:23	Antibacterial properties of laser-encapsulated titanium oxide nanotubes	0:70	Antibacterial properties of laser-encapsulated titanium oxide nanotubes decorated with nanosilver and covered with chitosan/Eudragit polymers.
35913239	2	43	theme	modified	590:597	arg1	surface					599:605	such a modified surface	583:605	such a modified surface	583:605	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	3	44	theme	substance	659:667	arg1	release					631:637	a controlled release	618:637	a controlled release of the bactericidal substance	618:667	To ensure a controlled release of the bactericidal substance, the samples were additionally coated with a pH-sensitive chitosan/Eudragit 100 coating, also exhibiting bactericidal properties.
35913239	5	45	theme	different	1039:1047	arg1	pH					1049:1050	different pH	1039:1050	different pH	1039:1050	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	1	46	from	oxidation	235:243	arg1	electrolyte					296:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	7	47	theme	simulated	1291:1299	arg1	solution					1312:1319	a simulated body fluid solution	1289:1319	a simulated body fluid solution at acidic pH	1289:1332	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	7	48	theme	burst	1434:1438	arg1	effect					1440:1445	exposure avoiding burst effect	1416:1445	exposure avoiding burst effect	1416:1445	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	5	49	theme	AgNPs	992:996	arg1	adhesion					935:942	adhesion	935:942	adhesion of the coating to the substrate	935:974	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	49	theme	AgNPs	992:996	arg1	wettability					900:910	The wettability	896:910	The wettability	896:910	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	49	theme	AgNPs	992:996	arg1	properties					923:932	corrosion properties	913:932	corrosion properties	913:932	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	49	theme	AgNPs	992:996	arg1	release					981:987	the release	977:987	the release of AgNPs into solutions simulating body fluids at different pH	977:1050	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	5	49	theme	AgNPs	992:996	arg1	properties					1071:1080	antibacterial properties	1057:1080	antibacterial properties	1057:1080	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	1	50	theme	antibacterial	154:166	arg1	properties					168:177	antibacterial properties	154:177	antibacterial properties	154:177	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	1	51	theme	diethylene	272:281	arg1	electrolyte					296:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	4	52	theme	titanium	812:819	arg1	samples					821:827	The modified titanium samples	799:827	The modified titanium samples	799:827	The modified titanium samples were characterized using SEM, EDS, AFM, Raman, and XPS techniques.
35913239	8	53	theme	Gram-positive	1501:1513	arg1	bacteria					1533:1540	both Gram-positive and Gram-negative bacteria	1496:1540	both Gram-positive and Gram-negative bacteria	1496:1540	The proposed modification was effective against both Gram-positive and Gram-negative bacteria.
35913239	7	54	theme	effect	1440:1445	arg1	days					1408:1411	three days	1402:1411	three days of exposure avoiding burst effect	1402:1445	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	7	55	theme	metallic	1265:1272	arg1	particles					1274:1282	metallic particles	1265:1282	metallic particles	1265:1282	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	1	56	theme	glycol-based	283:294	arg1	electrolyte					296:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	the fluoride-containing diethylene glycol-based electrolyte	248:306	To provide antibacterial properties, the titanium samples were subjected to electrochemical oxidation in the fluoride-containing diethylene glycol-based electrolyte to create a titanium oxide nanotubular surface.
35913239	7	57	theme	silver	1238:1243	arg1	silver					1238:1243	silver	1238:1243	silver	1238:1243	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	7	57	theme	silver	1238:1243	arg1	amount					1228:1233	the amount	1224:1233	the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH	1224:1332	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	5	58	theme	antibacterial	1057:1069	arg1	properties					1071:1080	antibacterial properties	1057:1080	antibacterial properties	1057:1080	The wettability, corrosion properties, adhesion of the coating to the substrate, the release of AgNPs into solutions simulating body fluids at different pH, and antibacterial properties were further investigated.
35913239	3	59	theme	controlled	620:629	arg1	release					631:637	a controlled release	618:637	a controlled release of the bactericidal substance	618:667	To ensure a controlled release of the bactericidal substance, the samples were additionally coated with a pH-sensitive chitosan/Eudragit 100 coating, also exhibiting bactericidal properties.
35913239	7	60	from	pH	1331:1332	arg1	solution					1312:1319	a simulated body fluid solution	1289:1319	a simulated body fluid solution at acidic pH	1289:1332	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	6	61	theme	composite	1122:1130	arg1	hydrophilic					1146:1156	hydrophilic	1146:1156	hydrophilic	1146:1156	The obtained composite coatings were hydrophilic, adjacent to the surface, and corrosion-resistant.
35913239	6	61	theme	composite	1122:1130	arg1	coatings					1132:1139	The obtained composite coatings	1109:1139	The obtained composite coatings	1109:1139	The obtained composite coatings were hydrophilic, adjacent to the surface, and corrosion-resistant.
35913239	2	62	theme	nanoparticles	530:542	arg1	appearance					509:518	an appearance	506:518	an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface	506:605	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	3	63	theme	pH-sensitive	714:725	arg1	coating					749:755	a pH-sensitive chitosan/Eudragit 100 coating	712:755	a pH-sensitive chitosan/Eudragit 100 coating	712:755	To ensure a controlled release of the bactericidal substance, the samples were additionally coated with a pH-sensitive chitosan/Eudragit 100 coating, also exhibiting bactericidal properties.
35913239	7	64	theme	modified	1351:1358	arg1	samples					1360:1366	modified samples	1351:1366	modified samples with the biopolymer coating after three days of exposure avoiding burst effect	1351:1445	An increase in the amount of silver released as ions or metallic particles into a simulated body fluid solution at acidic pH was observed for modified samples with the biopolymer coating after three days of exposure avoiding burst effect.
35913239	6	65	theme	obtained	1113:1120	arg1	hydrophilic					1146:1156	hydrophilic	1146:1156	hydrophilic	1146:1156	The obtained composite coatings were hydrophilic, adjacent to the surface, and corrosion-resistant.
35913239	6	65	theme	obtained	1113:1120	arg1	coatings					1132:1139	The obtained composite coatings	1109:1139	The obtained composite coatings	1109:1139	The obtained composite coatings were hydrophilic, adjacent to the surface, and corrosion-resistant.
35913239	2	66	from	diameter	571:578	arg1	nanoparticles					530:542	silver nanoparticles	523:542	silver nanoparticles (AgNPs) of around 30 nm in diameter	523:578	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
35913239	2	66	from	diameter	571:578	arg1	AgNPs					545:549	AgNPs	545:549	AgNPs	545:549	Afterward, the surface was covered by sputtering with silver 5 nm film, and the tops of the nanotubes were capped using laser treatment, resulting in an appearance of silver nanoparticles (AgNPs) of around 30 nm in diameter on such a modified surface.
36077562	2	0	from	mechanism	189:197	arg1	relief					228:233	inflammation relief	215:233	inflammation relief	215:233	Few studies have focused on the role and mechanism of emulsions in inflammation relief.
36077562	3	1	theme	Pickering	310:318	arg1	emulsions					320:328	the novel and natural Pickering emulsions	288:328	the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles	288:378	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	12	2	theme	inflammasome	1603:1614	arg1	activation					1585:1594	the activation	1581:1594	the activation of the inflammasome	1581:1614	Therefore, it can be concluded that the LPNE may inhibit the activation of the inflammasome by decreasing LDH activity, potassium and chloride ions through binding with compositions of NLRP3.
36077562	2	3	dep	role	180:183	arg1	the					176:178	the	176:178	the	176:178	Few studies have focused on the role and mechanism of emulsions in inflammation relief.
36077562	5	4	theme	different	660:668	arg1	doses					670:674	different doses	660:674	different doses of the emulsion	660:690	Then, the inflammasome stimulation by different doses of the emulsion was tested in RAW 264.7 and THP-1 cells.
36077562	11	5	theme	I-conjugated	1415:1426	arg1	chitosan					1428:1435	parasin I-conjugated chitosan	1407:1435	parasin I-conjugated chitosan	1407:1435	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	5	6	theme	THP-1	720:724	arg1	cells					726:730	RAW 264.7 and THP-1 cells	706:730	cells	726:730	Then, the inflammasome stimulation by different doses of the emulsion was tested in RAW 264.7 and THP-1 cells.
36077562	2	7	theme	Few	148:150	arg1	studies					152:158	Few studies	148:158	Few studies	148:158	Few studies have focused on the role and mechanism of emulsions in inflammation relief.
36077562	1	8	theme	Antibacterial	56:68	arg1	emulsions					79:87	Antibacterial delivery emulsions	56:87	Antibacterial delivery emulsions	56:87	Antibacterial delivery emulsions are potential materials for treating bacterial infections.
36077562	1	8	theme	Antibacterial	56:68	arg1	materials					103:111	potential materials	93:111	potential materials for treating bacterial infections	93:145	Antibacterial delivery emulsions are potential materials for treating bacterial infections.
36077562	10	9	dep	6	1325:1325	arg1	to					1322:1323	to	1322:1323	to	1322:1323	However, from 1.5 to 6 mg/mL, LPNE had a dose-dependent effect on the release of casp-1.
36077562	10	10	contain	had	1339:1341	arg1	LPNE					1334:1337	LPNE	1334:1337	LPNE	1334:1337	However, from 1.5 to 6 mg/mL, LPNE had a dose-dependent effect on the release of casp-1.
36077562	10	10	contain	had	1339:1341	arg2	effect					1360:1365	a dose-dependent effect	1343:1365	a dose-dependent effect	1343:1365	However, from 1.5 to 6 mg/mL, LPNE had a dose-dependent effect on the release of casp-1.
36077562	9	11	theme	peptide-embedded	1200:1215	arg1	CPENE					1241:1245	CPENE	1241:1245	CPENE	1241:1245	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	9	11	theme	peptide-embedded	1200:1215	arg1	emulsion					1231:1238	the chitosan peptide-embedded nanoparticles emulsion	1187:1238	the chitosan peptide-embedded nanoparticles emulsion (CPENE)	1187:1246	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	10	12	theme	dose-dependent	1345:1358	arg1	effect					1360:1365	a dose-dependent effect	1343:1365	a dose-dependent effect	1343:1365	However, from 1.5 to 6 mg/mL, LPNE had a dose-dependent effect on the release of casp-1.
36077562	6	13	theme	IL-1β	786:790	arg1	expression					792:801	IL-1β expression	786:801	IL-1β expression	786:801	Finally, in Kunming mice with peritonitis, NLRP3 and IL-1β expression in the peritoneum were evaluated.
36077562	1	14	theme	delivery	70:77	arg1	emulsions					79:87	Antibacterial delivery emulsions	56:87	Antibacterial delivery emulsions	56:87	Antibacterial delivery emulsions are potential materials for treating bacterial infections.
36077562	1	14	theme	delivery	70:77	arg1	materials					103:111	potential materials	93:111	potential materials for treating bacterial infections	93:145	Antibacterial delivery emulsions are potential materials for treating bacterial infections.
36077562	3	15	theme	antimicrobial	344:356	arg1	nanoparticles					366:378	antimicrobial peptide nanoparticles	344:378	antimicrobial peptide nanoparticles	344:378	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	9	16	theme	lipid/peptide	1252:1264	arg1	nanoparticles					1266:1278	lipid/peptide nanoparticles	1252:1278	lipid/peptide nanoparticles	1252:1278	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	6	17	with	mice	753:756	arg1	peritonitis					763:773	peritonitis	763:773	peritonitis	763:773	Finally, in Kunming mice with peritonitis, NLRP3 and IL-1β expression in the peritoneum were evaluated.
36077562	12	18	theme	LDH	1630:1632	arg1	activity					1634:1641	LDH activity	1630:1641	LDH activity	1630:1641	Therefore, it can be concluded that the LPNE may inhibit the activation of the inflammasome by decreasing LDH activity, potassium and chloride ions through binding with compositions of NLRP3.
36077562	3	19	theme	peptide	358:364	arg1	nanoparticles					366:378	antimicrobial peptide nanoparticles	344:378	antimicrobial peptide nanoparticles	344:378	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	3	20	theme	previous	260:267	arg1	analysis					269:276	our previous analysis	256:276	our previous analysis	256:276	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	0	21	theme	Nanoparticle	0:11	arg1	Emulsions					13:21	Nanoparticle Emulsions	0:21	Nanoparticle Emulsions	0:21	Nanoparticle Emulsions Enhance the Inhibition of NLRP3.
36077562	9	22	theme	emulsion	1280:1287	arg1	groups					1296:1301	emulsion (LPNE) groups	1280:1301	emulsion (LPNE) groups	1280:1301	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	5	23	theme	inflammasome	632:643	arg1	stimulation					645:655	the inflammasome stimulation	628:655	the inflammasome stimulation by different doses of the emulsion	628:690	Then, the inflammasome stimulation by different doses of the emulsion was tested in RAW 264.7 and THP-1 cells.
36077562	1	24	theme	potential	93:101	arg1	emulsions					79:87	Antibacterial delivery emulsions	56:87	Antibacterial delivery emulsions	56:87	Antibacterial delivery emulsions are potential materials for treating bacterial infections.
36077562	1	24	theme	potential	93:101	arg1	materials					103:111	potential materials	93:111	potential materials for treating bacterial infections	93:145	Antibacterial delivery emulsions are potential materials for treating bacterial infections.
36077562	5	25	theme	emulsion	683:690	arg1	doses					670:674	different doses	660:674	different doses of the emulsion	660:690	Then, the inflammasome stimulation by different doses of the emulsion was tested in RAW 264.7 and THP-1 cells.
36077562	3	26	theme	regulation	399:408	arg1	effect					410:415	the regulation effect	395:415	the regulation effect of emulsion on inflammasome	395:443	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	8	27	theme	dose-dependent	1015:1028	arg1	effect					1041:1046	a dose-dependent inhibition effect	1013:1046	a dose-dependent inhibition effect on the release of IL-1β and casp-1	1013:1081	LPNE showed a dose-dependent inhibition effect on the release of IL-1β and casp-1.
36077562	9	28	dep	emulsion	1231:1238	arg1	groups					1296:1301	emulsion (LPNE) groups	1280:1301	emulsion (LPNE) groups	1280:1301	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	6	29	from	expression	792:801	arg1	peritoneum					810:819	the peritoneum	806:819	the peritoneum	806:819	Finally, in Kunming mice with peritonitis, NLRP3 and IL-1β expression in the peritoneum were evaluated.
36077562	12	30	theme	chloride	1658:1665	arg1	ions					1667:1670	chloride ions	1658:1670	chloride ions	1658:1670	Therefore, it can be concluded that the LPNE may inhibit the activation of the inflammasome by decreasing LDH activity, potassium and chloride ions through binding with compositions of NLRP3.
36077562	4	31	theme	parasin	553:559	arg1	I					561:561	parasin I or Pickering emulsion	553:583	I	561:561	Firstly, the interactions between inflammasome components and parasin I or Pickering emulsion were predicted by molecular docking.
36077562	4	32	theme	Pickering	566:574	arg1	emulsion					576:583	parasin I or Pickering emulsion	553:583	emulsion	576:583	Firstly, the interactions between inflammasome components and parasin I or Pickering emulsion were predicted by molecular docking.
36077562	9	33	theme	LDH	1161:1163	arg1	activity					1165:1172	the LDH activity	1157:1172	the LDH activity	1157:1172	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	3	34	theme	emulsion	420:427	arg1	effect					410:415	the regulation effect	395:415	the regulation effect of emulsion on inflammasome	395:443	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	6	35	from	NLRP3	776:780	arg1	peritoneum					810:819	the peritoneum	806:819	the peritoneum	806:819	Finally, in Kunming mice with peritonitis, NLRP3 and IL-1β expression in the peritoneum were evaluated.
36077562	4	36	theme	inflammasome	525:536	arg1	components					538:547	inflammasome components	525:547	inflammasome components	525:547	Firstly, the interactions between inflammasome components and parasin I or Pickering emulsion were predicted by molecular docking.
36077562	11	37	theme	chloride	1509:1516	arg1	ions					1518:1521	potassium and chloride ions	1495:1521	potassium and chloride ions	1495:1521	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	11	38	theme	ions	1518:1521	arg1	release					1484:1490	the release	1480:1490	the release of potassium and chloride ions	1480:1521	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	8	39	theme	IL-1β	1066:1070	arg1	release					1055:1061	the release	1051:1061	the release of IL-1β and casp-1	1051:1081	LPNE showed a dose-dependent inhibition effect on the release of IL-1β and casp-1.
36077562	9	40	theme	LPNE	1290:1293	arg1	groups					1296:1301	emulsion (LPNE) groups	1280:1301	emulsion (LPNE) groups	1280:1301	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	6	41	theme	Kunming	745:751	arg1	mice					753:756	Kunming mice	745:756	Kunming mice with peritonitis	745:773	Finally, in Kunming mice with peritonitis, NLRP3 and IL-1β expression in the peritoneum were evaluated.
36077562	1	42	theme	bacterial	126:134	arg1	infections					136:145	bacterial infections	126:145	bacterial infections	126:145	Antibacterial delivery emulsions are potential materials for treating bacterial infections.
36077562	0	43	theme	NLRP3	49:53	arg1	Inhibition					35:44	the Inhibition	31:44	the Inhibition of NLRP3	31:53	Nanoparticle Emulsions Enhance the Inhibition of NLRP3.
36077562	3	44	theme	natural	302:308	arg1	emulsions					320:328	the novel and natural Pickering emulsions	288:328	the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles	288:378	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	2	45	theme	inflammation	215:226	arg1	relief					228:233	inflammation relief	215:233	inflammation relief	215:233	Few studies have focused on the role and mechanism of emulsions in inflammation relief.
36077562	10	46	theme	casp-1	1385:1390	arg1	release					1374:1380	the release	1370:1380	the release of casp-1	1370:1390	However, from 1.5 to 6 mg/mL, LPNE had a dose-dependent effect on the release of casp-1.
36077562	11	47	theme	potassium	1495:1503	arg1	ions					1518:1521	potassium and chloride ions	1495:1521	potassium and chloride ions	1495:1521	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	8	48	theme	casp-1	1076:1081	arg1	release					1055:1061	the release	1051:1061	the release of IL-1β and casp-1	1051:1081	LPNE showed a dose-dependent inhibition effect on the release of IL-1β and casp-1.
36077562	9	49	theme	chitosan	1191:1198	arg1	CPENE					1241:1245	CPENE	1241:1245	CPENE	1241:1245	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	9	49	theme	chitosan	1191:1198	arg1	emulsion					1231:1238	the chitosan peptide-embedded nanoparticles emulsion	1187:1238	the chitosan peptide-embedded nanoparticles emulsion (CPENE)	1187:1246	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	7	50	theme	NLRP3	961:965	arg1	assembly					945:952	the assembly	941:952	the assembly of the NLRP3	941:965	The results showed that the Pickering emulsion could combine with ALK, casp-1, NEK7, or NLRP3 to affect the assembly of the NLRP3 and further relieve inflammation.
36077562	11	51	theme	parasin	1407:1413	arg1	chitosan					1428:1435	parasin I-conjugated chitosan	1407:1435	parasin I-conjugated chitosan	1407:1435	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	3	52	theme	novel	292:296	arg1	emulsions					320:328	the novel and natural Pickering emulsions	288:328	the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles	288:378	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	2	53	theme	emulsions	202:210	arg1	mechanism					189:197	mechanism	189:197	mechanism	189:197	Few studies have focused on the role and mechanism of emulsions in inflammation relief.
36077562	2	53	theme	emulsions	202:210	arg1	role					180:183	role	180:183	role	180:183	Few studies have focused on the role and mechanism of emulsions in inflammation relief.
36077562	11	54	theme	nanoparticles	1437:1449	arg1	PCNE					1461:1464	PCNE	1461:1464	PCNE	1461:1464	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	11	54	theme	nanoparticles	1437:1449	arg1	emulsion					1451:1458	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion	1393:1458	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE)	1393:1465	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	4	55	theme	molecular	603:611	arg1	docking					613:619	molecular docking	603:619	molecular docking	603:619	Firstly, the interactions between inflammasome components and parasin I or Pickering emulsion were predicted by molecular docking.
36077562	2	56	from	role	180:183	arg1	relief					228:233	inflammation relief	215:233	inflammation relief	215:233	Few studies have focused on the role and mechanism of emulsions in inflammation relief.
36077562	9	57	theme	nanoparticles	1217:1229	arg1	CPENE					1241:1245	CPENE	1241:1245	CPENE	1241:1245	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	9	57	theme	nanoparticles	1217:1229	arg1	emulsion					1231:1238	the chitosan peptide-embedded nanoparticles emulsion	1187:1238	the chitosan peptide-embedded nanoparticles emulsion (CPENE)	1187:1246	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	11	58	theme	chitosan	1428:1435	arg1	nanoparticles					1437:1449	The CPENE and parasin I-conjugated chitosan nanoparticles	1393:1449	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE)	1393:1465	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	9	59	theme	parasin	1110:1116	arg1	concentration					1093:1105	the concentration	1089:1105	the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL	1089:1154	With the concentration of parasin I increased from 1.5 mg/mL to 3 mg/mL, the LDH activity decreased in the chitosan peptide-embedded nanoparticles emulsion (CPENE) and lipid/peptide nanoparticles emulsion (LPNE) groups.
36077562	11	60	theme	CPENE	1397:1401	arg1	nanoparticles					1437:1449	The CPENE and parasin I-conjugated chitosan nanoparticles	1393:1449	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE)	1393:1465	The CPENE and parasin I-conjugated chitosan nanoparticles emulsion (PCNE) may decrease the release of potassium and chloride ions.
36077562	3	61	from	effect	410:415	arg1	inflammasome					432:443	inflammasome	432:443	inflammasome	432:443	Therefore, based on our previous analysis, in which the novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were prepared, the regulation effect of emulsion on inflammasome was explored in silico, in vitro and in vivo.
36077562	8	62	theme	inhibition	1030:1039	arg1	effect					1041:1046	a dose-dependent inhibition effect	1013:1046	a dose-dependent inhibition effect on the release of IL-1β and casp-1	1013:1081	LPNE showed a dose-dependent inhibition effect on the release of IL-1β and casp-1.
36077562	12	63	theme	NLRP3	1709:1713	arg1	compositions					1693:1704	compositions	1693:1704	compositions of NLRP3	1693:1713	Therefore, it can be concluded that the LPNE may inhibit the activation of the inflammasome by decreasing LDH activity, potassium and chloride ions through binding with compositions of NLRP3.
36077562	7	64	theme	Pickering	865:873	arg1	emulsion					875:882	the Pickering emulsion	861:882	the Pickering emulsion	861:882	The results showed that the Pickering emulsion could combine with ALK, casp-1, NEK7, or NLRP3 to affect the assembly of the NLRP3 and further relieve inflammation.
36077562	8	65	from	effect	1041:1046	arg1	release					1055:1061	the release	1051:1061	the release of IL-1β and casp-1	1051:1081	LPNE showed a dose-dependent inhibition effect on the release of IL-1β and casp-1.
35711960	3	0	theme	β1,4-galactosyl	582:596	arg1	moieties					618:625	the β1,4-galactosyl and β1,3-galactosyl moieties	578:625	the β1,4-galactosyl and β1,3-galactosyl moieties	578:625	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	4	1	theme	target	869:874	arg1	LNH					876:878	the target LNH	865:878	the target LNH	865:878	Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
35711960	3	2	dep	C6-arm	666:671	arg1	the					662:664	the	662:664	the	662:664	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	4	3	theme	milk	810:813	arg1	Lacto-N-neotetraose					761:779	Lacto-N-neotetraose	761:779	Lacto-N-neotetraose (LNnH)	761:786	Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
35711960	4	3	theme	milk	810:813	arg1	oligosaccharide					815:829	another common human milk oligosaccharide	789:829	another common human milk oligosaccharide	789:829	Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
35711960	1	4	theme	chemical	188:195	arg1	synthesis					210:218	chemical carbohydrate synthesis	188:218	chemical carbohydrate synthesis	188:218	We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy.
35711960	2	5	theme	GlcNAcβ1→6	318:327	arg1	Galβ1→4Glc					330:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	5	theme	GlcNAcβ1→6	318:327	arg1	precursor					348:356	a key precursor	342:356	a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	342:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	1	6	theme	carbohydrate	197:208	arg1	synthesis					210:218	chemical carbohydrate synthesis	188:218	chemical carbohydrate synthesis	188:218	We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy.
35711960	0	7	theme	Chemoenzymatic	0:13	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	0:93	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.
35711960	2	8	theme	milk	437:440	arg1	oligosaccharides					442:457	asymmetrically branched human milk oligosaccharides	407:457	asymmetrically branched human milk oligosaccharides	407:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	9	theme	key	344:346	arg1	Galβ1→4Glc					330:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	9	theme	key	344:346	arg1	precursor					348:356	a key precursor	342:356	a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	342:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	3	10	theme	reactions	540:548	arg1	order					499:503	the order	495:503	the order of galactosyltransferase-catalyzed reactions	495:548	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	3	11	theme	efficient	736:744	arg1	synthesis					750:758	an efficient LNH synthesis	733:758	an efficient LNH synthesis	733:758	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	1	12	theme	chemoenzymatic	125:138	arg1	synthesis					140:148	the first chemoenzymatic synthesis	115:148	the first chemoenzymatic synthesis of lacto-N-hexaose (LNH)	115:173	We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy.
35711960	3	13	theme	β1,3-galactosyl	602:616	arg1	moieties					618:625	the β1,4-galactosyl and β1,3-galactosyl moieties	578:625	the β1,4-galactosyl and β1,3-galactosyl moieties	578:625	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	0	14	theme	Human	52:56	arg1	Lacto-N-Hexaose					79:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.
35711960	4	15	theme	common	797:802	arg1	Lacto-N-neotetraose					761:779	Lacto-N-neotetraose	761:779	Lacto-N-neotetraose (LNnH)	761:786	Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
35711960	4	15	theme	common	797:802	arg1	oligosaccharide					815:829	another common human milk oligosaccharide	789:829	another common human milk oligosaccharide	789:829	Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
35711960	3	16	theme	LNH	746:748	arg1	synthesis					750:758	an efficient LNH synthesis	733:758	an efficient LNH synthesis	733:758	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	2	17	theme	tetrasaccharide	275:289	arg1	Galβ1→4Glc					330:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	17	theme	tetrasaccharide	275:289	arg1	precursor					348:356	a key precursor	342:356	a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	342:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	1	18	theme	enzymatic	239:247	arg1	strategy					263:270	a selectively enzymatic glycosylation strategy	225:270	a selectively enzymatic glycosylation strategy	225:270	We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy.
35711960	0	19	theme	Branched	43:50	arg1	Lacto-N-Hexaose					79:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.
35711960	3	20	theme	tetrasaccharide	691:705	arg1	C3-arm					677:682	C3-arm	677:682	C3-arm	677:682	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	3	20	theme	tetrasaccharide	691:705	arg1	tetrasaccharide					691:705	the tetrasaccharide	687:705	the tetrasaccharide	687:705	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	3	20	theme	tetrasaccharide	691:705	arg1	C6-arm					666:671	C6-arm	666:671	C6-arm	666:671	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	1	21	theme	glycosylation	249:261	arg1	strategy					263:270	a selectively enzymatic glycosylation strategy	225:270	a selectively enzymatic glycosylation strategy	225:270	We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy.
35711960	0	22	theme	Oligosaccharide	63:77	arg1	Lacto-N-Hexaose					79:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.
35711960	4	23	theme	human	804:808	arg1	Lacto-N-neotetraose					761:779	Lacto-N-neotetraose	761:779	Lacto-N-neotetraose (LNnH)	761:786	Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
35711960	4	23	theme	human	804:808	arg1	oligosaccharide					815:829	another common human milk oligosaccharide	789:829	another common human milk oligosaccharide	789:829	Lacto-N-neotetraose (LNnH), another common human milk oligosaccharide, was also synthesized en route to the target LNH.
35711960	0	24	theme	Milk	58:61	arg1	Lacto-N-Hexaose					79:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	28:93	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.
35711960	2	25	theme	glycan	383:388	arg1	extension					390:398	subsequent enzymatic glycan extension	362:398	subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	362:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	1	26	theme	lacto-N-hexaose	153:167	arg1	synthesis					140:148	the first chemoenzymatic synthesis	115:148	the first chemoenzymatic synthesis of lacto-N-hexaose (LNH)	115:173	We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy.
35711960	1	27	theme	first	119:123	arg1	synthesis					140:148	the first chemoenzymatic synthesis	115:148	the first chemoenzymatic synthesis of lacto-N-hexaose (LNH)	115:173	We herein reported the first chemoenzymatic synthesis of lacto-N-hexaose (LNH) by combining chemical carbohydrate synthesis with a selectively enzymatic glycosylation strategy.
35711960	2	28	theme	GlcNH2β1→3	306:315	arg1	Galβ1→4Glc					330:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	28	theme	GlcNH2β1→3	306:315	arg1	precursor					348:356	a key precursor	342:356	a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	342:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	29	theme	subsequent	362:371	arg1	extension					390:398	subsequent enzymatic glycan extension	362:398	subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	362:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	30	theme	enzymatic	373:381	arg1	extension					390:398	subsequent enzymatic glycan extension	362:398	subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	362:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	3	31	theme	galactosyltransferase-catalyzed	508:538	arg1	reactions					540:548	galactosyltransferase-catalyzed reactions	508:548	galactosyltransferase-catalyzed reactions	508:548	When the order of galactosyltransferase-catalyzed reactions was appropriately arranged, the β1,4-galactosyl and β1,3-galactosyl moieties could be sequentially assembled on the C6-arm and C3-arm of the tetrasaccharide, respectively, to achieve an efficient LNH synthesis.
35711960	2	32	theme	structure	296:304	arg1	Galβ1→4Glc					330:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	32	theme	structure	296:304	arg1	precursor					348:356	a key precursor	342:356	a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	342:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	33	theme	human	431:435	arg1	oligosaccharides					442:457	asymmetrically branched human milk oligosaccharides	407:457	asymmetrically branched human milk oligosaccharides	407:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	0	34	theme	Lacto-N-Hexaose	79:93	arg1	Synthesis					15:23	Chemoenzymatic Synthesis	0:23	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose	0:93	Chemoenzymatic Synthesis of Asymmetrically Branched Human Milk Oligosaccharide Lacto-N-Hexaose.
35711960	2	35	theme	core	291:294	arg1	Galβ1→4Glc					330:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc	273:339	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	35	theme	core	291:294	arg1	precursor					348:356	a key precursor	342:356	a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides	342:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35711960	2	36	theme	branched	422:429	arg1	oligosaccharides					442:457	asymmetrically branched human milk oligosaccharides	407:457	asymmetrically branched human milk oligosaccharides	407:457	A tetrasaccharide core structure GlcNH2β1→3 (GlcNAcβ1→6) Galβ1→4Glc, a key precursor for subsequent enzymatic glycan extension toward asymmetrically branched human milk oligosaccharides, was synthesized in this work.
35169131	5	0	theme	malignant	680:688	arg1	behaviors					690:698	malignant behaviors	680:698	malignant behaviors of NB cells	680:710	Downregulation of C1GALT1 promotes malignant behaviors of NB cells in vitro and in vivo.
35169131	6	1	theme	Vicia	840:844	arg1	beads					865:869	Vicia villosa agglutinin beads	840:869	Vicia villosa agglutinin beads	840:869	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	2	2	theme	altered	352:358	arg1	expression					360:369	its altered expression	348:369	its altered expression	348:369	Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors.
35169131	4	3	theme	good	609:612	arg1	prognosis					614:622	good prognosis	609:622	good prognosis	609:622	Our data showed that C1GALT1 expression was significantly associated with differentiated tumor histology, correlated with TrkA expression, and predicted good prognosis independently in NB.
35169131	11	4	theme	novel	1720:1724	arg1	role					1737:1740	the novel prognostic role	1716:1740	the novel prognostic role of C1GALT1 in NB	1716:1757	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	6	5	theme	O-glycans	901:909	arg1	modulation					887:896	the modulation	883:896	the modulation of O-glycans on TrkA by C1GALT1	883:928	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	5	6	theme	cells	706:710	arg1	behaviors					690:698	malignant behaviors	680:698	malignant behaviors of NB cells	680:710	Downregulation of C1GALT1 promotes malignant behaviors of NB cells in vitro and in vivo.
35169131	5	7	theme	C1GALT1	663:669	arg1	Downregulation					645:658	Downregulation	645:658	Downregulation of C1GALT1	645:669	Downregulation of C1GALT1 promotes malignant behaviors of NB cells in vitro and in vivo.
35169131	1	8	theme	sympathoadrenal	180:194	arg1	lineage					196:202	the sympathoadrenal lineage	176:202	the sympathoadrenal lineage of the neural crest progenitor cells	176:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	8	9	theme	migration	1231:1239	arg1	effects					1220:1226	the inhibitory effects	1205:1226	the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells	1205:1287	Moreover, the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells were blocked by TrkA downregulation.
35169131	8	10	theme	TrkA	1305:1308	arg1	downregulation					1310:1323	TrkA downregulation	1305:1323	TrkA downregulation	1305:1323	Moreover, the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells were blocked by TrkA downregulation.
35169131	6	11	from	modulation	887:896	arg1	TrkA					914:917	TrkA	914:917	TrkA by C1GALT1	914:928	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	2	12	theme	crucial	299:305	arg1	step					307:310	the crucial step	295:310	the crucial step of GalNAc-type O-glycosylation	295:341	Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors.
35169131	8	13	theme	invasion	1245:1252	arg1	effects					1220:1226	the inhibitory effects	1205:1226	the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells	1205:1287	Moreover, the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells were blocked by TrkA downregulation.
35169131	1	14	theme	neural	211:216	arg1	cells					235:239	the neural crest progenitor cells	207:239	the neural crest progenitor cells	207:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	7	15	theme	TrkA	1138:1141	arg1	knockdown					1125:1133	knockdown	1125:1133	knockdown of TrkA	1125:1141	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	10	16	dep	structures	1627:1636	arg1	regulate					1653:1660	regulate	1653:1660	can regulate TrkA signaling in NB cells	1649:1687	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	7	17	theme	TrkA	1066:1069	arg1	levels					1056:1061	the protein levels	1044:1061	the protein levels of TrkA	1044:1069	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	11	18	theme	TrkA	1803:1806	arg1	information					1776:1786	new information	1772:1786	new information of C1GALT1 and TrkA on the pathogenesis of NB	1772:1832	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	0	19	from	signaling	95:103	arg1	neuroblastoma					108:120	neuroblastoma	108:120	neuroblastoma	108:120	C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
35169131	11	20	theme	C1GALT1	1791:1797	arg1	information					1776:1786	new information	1772:1786	new information of C1GALT1 and TrkA on the pathogenesis of NB	1772:1832	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	0	21	theme	malignant	65:73	arg1	phenotypes					75:84	malignant phenotypes	65:84	malignant phenotypes	65:84	C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
35169131	9	22	theme	C1GALT1	1326:1332	arg1	knockdown					1334:1342	C1GALT1 knockdown	1326:1342	C1GALT1 knockdown	1326:1342	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	2	23	theme	Core	242:245	arg1	C1GALT1					277:283	C1GALT1	277:283	C1GALT1	277:283	Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors.
35169131	2	23	theme	Core	242:245	arg1	β1,3-galactosyltransferase					249:274	Core 1 β1,3-galactosyltransferase	242:274	Core 1 β1,3-galactosyltransferase (C1GALT1)	242:284	Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors.
35169131	10	24	from	existence	1563:1571	arg1	TrkA					1601:1604	TrkA	1601:1604	TrkA	1601:1604	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	11	25	from	information	1776:1786	arg1	pathogenesis					1815:1826	the pathogenesis	1811:1826	the pathogenesis of NB	1811:1832	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	6	26	theme	cell	994:997	arg1	surface					999:1005	the NB cell surface	987:1005	the NB cell surface	987:1005	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	4	27	theme	C1GALT1	477:483	arg1	expression					485:494	C1GALT1 expression	477:494	C1GALT1 expression	477:494	Our data showed that C1GALT1 expression was significantly associated with differentiated tumor histology, correlated with TrkA expression, and predicted good prognosis independently in NB.
35169131	0	28	theme	C1GALT1	0:6	arg1	expression					8:17	C1GALT1 expression	0:17	C1GALT1 expression	0:17	C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
35169131	11	29	from	role	1737:1740	arg1	NB					1756:1757	NB	1756:1757	NB	1756:1757	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	4	30	theme	TrkA	578:581	arg1	expression					583:592	TrkA expression	578:592	TrkA expression	578:592	Our data showed that C1GALT1 expression was significantly associated with differentiated tumor histology, correlated with TrkA expression, and predicted good prognosis independently in NB.
35169131	1	31	attach	derived	163:169	arg2	tumor					157:161	a childhood tumor	145:161	a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells	145:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	1	31	attach	derived	163:169	arg2	Neuroblastoma					123:135	Neuroblastoma	123:135	Neuroblastoma (NB)	123:140	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	1	31	attach	derived	163:169	arg1	lineage					196:202	the sympathoadrenal lineage	176:202	the sympathoadrenal lineage of the neural crest progenitor cells	176:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	0	32	theme	favorable	30:38	arg1	prognosis					40:48	a favorable prognosis	28:48	a favorable prognosis	28:48	C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
35169131	4	33	theme	differentiated	530:543	arg1	histology					551:559	differentiated tumor histology	530:559	differentiated tumor histology	530:559	Our data showed that C1GALT1 expression was significantly associated with differentiated tumor histology, correlated with TrkA expression, and predicted good prognosis independently in NB.
35169131	6	34	theme	C1GALT1	785:791	arg1	knockdown					772:780	knockdown	772:780	knockdown of C1GALT1 in NB cells	772:803	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	7	35	theme	C1GALT1-induced	1153:1167	arg1	differentiation					1178:1192	C1GALT1-induced neuronal differentiation	1153:1192	C1GALT1-induced neuronal differentiation	1153:1192	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	6	36	from	knockdown	772:780	arg1	cells					799:803	NB cells	796:803	NB cells	796:803	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	10	37	theme	O-glycans	1588:1596	arg1	existence					1563:1571	the existence	1559:1571	the existence of GalNAc-type O-glycans on TrkA	1559:1604	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	9	38	theme	C1GALT1-overexpressing	1449:1470	arg1	cells					1475:1479	C1GALT1-overexpressing NB cells	1449:1479	C1GALT1-overexpressing NB cells with TrkA knockdown	1449:1499	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	6	39	theme	agglutinin	854:863	arg1	beads					865:869	Vicia villosa agglutinin beads	840:869	Vicia villosa agglutinin beads	840:869	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	11	40	theme	new	1772:1774	arg1	information					1776:1786	new information	1772:1786	new information of C1GALT1 and TrkA on the pathogenesis of NB	1772:1832	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	2	41	theme	cancer	379:384	arg1	behaviors					386:394	cancer behaviors	379:394	cancer behaviors	379:394	Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors.
35169131	1	42	theme	cells	235:239	arg1	lineage					196:202	the sympathoadrenal lineage	176:202	the sympathoadrenal lineage of the neural crest progenitor cells	176:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	6	43	theme	silencing	935:943	arg1	C1GALT1					945:951	silencing C1GALT1	935:951	silencing C1GALT1	935:951	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	6	44	theme	Mechanistic	734:744	arg1	investigation					746:758	Mechanistic investigation	734:758	Mechanistic investigation	734:758	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	9	45	theme	TrkA	1486:1489	arg1	knockdown					1491:1499	TrkA knockdown	1486:1499	TrkA knockdown	1486:1499	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	8	46	from	effects	1220:1226	arg1	cells					1283:1287	C1GALT1-overexpressing NB cells	1257:1287	C1GALT1-overexpressing NB cells	1257:1287	Moreover, the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells were blocked by TrkA downregulation.
35169131	7	47	theme	cells	1110:1114	arg1	differentiation					1088:1102	the differentiation	1084:1102	the differentiation of NB cells	1084:1114	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	8	48	theme	NB	1280:1281	arg1	cells					1283:1287	C1GALT1-overexpressing NB cells	1257:1287	C1GALT1-overexpressing NB cells	1257:1287	Moreover, the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells were blocked by TrkA downregulation.
35169131	10	49	theme	NB	1680:1681	arg1	cells					1683:1687	NB cells	1680:1687	NB cells	1680:1687	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	11	50	theme	prognostic	1726:1735	arg1	role					1737:1740	the novel prognostic role	1716:1740	the novel prognostic role of C1GALT1 in NB	1716:1757	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	2	51	theme	GalNAc-type	315:325	arg1	O-glycosylation					327:341	GalNAc-type O-glycosylation	315:341	GalNAc-type O-glycosylation	315:341	Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors.
35169131	8	52	theme	inhibitory	1209:1218	arg1	effects					1220:1226	the inhibitory effects	1205:1226	the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells	1205:1287	Moreover, the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells were blocked by TrkA downregulation.
35169131	6	53	theme	NB	991:992	arg1	surface					999:1005	the NB cell surface	987:1005	the NB cell surface	987:1005	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	7	54	theme	NB	1107:1108	arg1	cells					1110:1114	NB cells	1107:1114	NB cells	1107:1114	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	6	55	theme	NB	796:797	arg1	cells					799:803	NB cells	796:803	NB cells	796:803	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	6	56	theme	TrkA	968:971	arg1	expression					973:982	the TrkA expression	964:982	the TrkA expression on the NB cell surface	964:1005	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	5	57	theme	NB	703:704	arg1	cells					706:710	NB cells	703:710	NB cells	703:710	Downregulation of C1GALT1 promotes malignant behaviors of NB cells in vitro and in vivo.
35169131	7	58	theme	protein	1048:1054	arg1	levels					1056:1061	the protein levels	1044:1061	the protein levels of TrkA	1044:1069	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	7	59	theme	C1GALT1	1026:1032	arg1	Overexpression					1008:1021	Overexpression	1008:1021	Overexpression of C1GALT1	1008:1032	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	10	60	theme	TrkA	1662:1665	arg1	signaling					1667:1675	TrkA signaling	1662:1675	TrkA signaling	1662:1675	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	1	61	theme	crest	218:222	arg1	cells					235:239	the neural crest progenitor cells	207:239	the neural crest progenitor cells	207:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	0	62	theme	TrkA	90:93	arg1	signaling					95:103	TrkA signaling	90:103	TrkA signaling in neuroblastoma	90:120	C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
35169131	3	63	from	role	410:413	arg1	tumors					432:437	NB tumors	429:437	NB tumors	429:437	However, the role of C1GALT1 in NB tumors remains unclear.
35169131	9	64	from	consistent	1435:1444	arg1	cells					1475:1479	C1GALT1-overexpressing NB cells	1449:1479	C1GALT1-overexpressing NB cells with TrkA knockdown	1449:1499	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	1	65	theme	childhood	147:155	arg1	Neuroblastoma					123:135	Neuroblastoma	123:135	Neuroblastoma (NB)	123:140	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	1	65	theme	childhood	147:155	arg1	tumor					157:161	a childhood tumor	145:161	a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells	145:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	10	66	from	O-glycans	1588:1596	arg1	TrkA					1601:1604	TrkA	1601:1604	TrkA	1601:1604	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	11	67	theme	NB	1831:1832	arg1	pathogenesis					1815:1826	the pathogenesis	1811:1826	the pathogenesis of NB	1811:1832	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	9	68	theme	AKT	1353:1355	arg1	phosphorylation					1357:1371	AKT phosphorylation	1353:1371	AKT phosphorylation	1353:1371	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	10	69	from	TrkA	1601:1604	arg1	existence					1563:1571	the existence	1559:1571	the existence of GalNAc-type O-glycans on TrkA	1559:1604	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	10	70	theme	first	1540:1544	arg1	evidence					1546:1553	the first evidence	1536:1553	the first evidence for the existence of GalNAc-type O-glycans on TrkA	1536:1604	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	1	71	theme	progenitor	224:233	arg1	cells					235:239	the neural crest progenitor cells	207:239	the neural crest progenitor cells	207:239	Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of the neural crest progenitor cells.
35169131	7	72	dep	increased	1034:1042	arg1	whereas					1117:1123	whereas	1117:1123	whereas	1117:1123	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	9	73	theme	ERK	1388:1390	arg1	phosphorylation					1392:1406	ERK phosphorylation	1388:1406	ERK phosphorylation	1388:1406	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	3	74	theme	NB	429:430	arg1	tumors					432:437	NB tumors	429:437	NB tumors	429:437	However, the role of C1GALT1 in NB tumors remains unclear.
35169131	6	75	from	expression	973:982	arg1	surface					999:1005	the NB cell surface	987:1005	the NB cell surface	987:1005	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	7	76	theme	neuronal	1169:1176	arg1	differentiation					1178:1192	C1GALT1-induced neuronal differentiation	1153:1192	C1GALT1-induced neuronal differentiation	1153:1192	Overexpression of C1GALT1 increased the protein levels of TrkA and promoted the differentiation of NB cells, whereas knockdown of TrkA inhibited C1GALT1-induced neuronal differentiation.
35169131	3	77	theme	C1GALT1	418:424	arg1	role					410:413	the role	406:413	the role of C1GALT1 in NB tumors	406:437	However, the role of C1GALT1 in NB tumors remains unclear.
35169131	4	78	theme	tumor	545:549	arg1	histology					551:559	differentiated tumor histology	530:559	differentiated tumor histology	530:559	Our data showed that C1GALT1 expression was significantly associated with differentiated tumor histology, correlated with TrkA expression, and predicted good prognosis independently in NB.
35169131	11	79	theme	C1GALT1	1745:1751	arg1	role					1737:1740	the novel prognostic role	1716:1740	the novel prognostic role of C1GALT1 in NB	1716:1757	This study sheds light on the novel prognostic role of C1GALT1 in NB and provides new information of C1GALT1 and TrkA on the pathogenesis of NB.
35169131	9	80	from	cells	1475:1479	arg1	consistent					1435:1444	consistent	1435:1444	consistent	1435:1444	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	10	81	theme	GalNAc-type	1576:1586	arg1	O-glycans					1588:1596	GalNAc-type O-glycans	1576:1596	GalNAc-type O-glycans on TrkA	1576:1604	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
35169131	9	82	with	cells	1475:1479	arg1	knockdown					1491:1499	TrkA knockdown	1486:1499	TrkA knockdown	1486:1499	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	2	83	theme	O-glycosylation	327:341	arg1	step					307:310	the crucial step	295:310	the crucial step of GalNAc-type O-glycosylation	295:341	Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors.
35169131	8	84	theme	C1GALT1-overexpressing	1257:1278	arg1	cells					1283:1287	C1GALT1-overexpressing NB cells	1257:1287	C1GALT1-overexpressing NB cells	1257:1287	Moreover, the inhibitory effects of migration and invasion in C1GALT1-overexpressing NB cells were blocked by TrkA downregulation.
35169131	9	85	theme	NB	1472:1473	arg1	cells					1475:1479	C1GALT1-overexpressing NB cells	1449:1479	C1GALT1-overexpressing NB cells with TrkA knockdown	1449:1499	C1GALT1 knockdown enhanced AKT phosphorylation but attenuated ERK phosphorylation, and these properties were consistent in C1GALT1-overexpressing NB cells with TrkA knockdown.
35169131	6	86	theme	villosa	846:852	arg1	beads					865:869	Vicia villosa agglutinin beads	840:869	Vicia villosa agglutinin beads	840:869	Mechanistic investigation showed that knockdown of C1GALT1 in NB cells increased TrkA pulled down through Vicia villosa agglutinin beads, indicating the modulation of O-glycans on TrkA by C1GALT1, and silencing C1GALT1 suppressed the TrkA expression on the NB cell surface.
35169131	10	87	theme	O-glycan	1618:1625	arg1	structures					1627:1636	O-glycan structures	1618:1636	O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells	1618:1687	Taken together, our data provided the first evidence for the existence of GalNAc-type O-glycans on TrkA and altered O-glycan structures by C1GALT1 can regulate TrkA signaling in NB cells.
36014377	0	0	theme	Cheese	79:84	arg1	Acids					28:32	Organic Acids	20:32	Organic Acids	20:32	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	0	0	theme	Cheese	79:84	arg1	Microstructure					38:51	Microstructure	38:51	Microstructure of Synbiotic Cheddar-Type Cheese	38:84	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	3	1	theme	acids	315:319	arg1	content					321:327	minerals and organic acids content	294:327	minerals and organic acids content	294:327	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	5	2	theme	electron	610:617	arg1	microscopy					619:628	Scanning electron microscopy	601:628	Scanning electron microscopy (SEM) of the cheese	601:648	Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure.
36014377	5	2	theme	electron	610:617	arg1	SEM					631:633	SEM	631:633	SEM	631:633	Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure.
36014377	5	3	theme	cheese	643:648	arg1	microscopy					619:628	Scanning electron microscopy	601:628	Scanning electron microscopy (SEM) of the cheese	601:648	Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure.
36014377	5	3	theme	cheese	643:648	arg1	SEM					631:633	SEM	631:633	SEM	631:633	Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure.
36014377	0	4	theme	Cheddar-Type	66:77	arg1	Cheese					79:84	Synbiotic Cheddar-Type Cheese	56:84	Synbiotic Cheddar-Type Cheese	56:84	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	6	5	theme	probiotic	773:781	arg1	viability					783:791	its probiotic viability	769:791	its probiotic viability	769:791	Meanwhile, inulin increased the likeliness of the cheese, and its probiotic viability remained above 107 colony forming unit (CFU) per gram during ripening.
36014377	1	6	theme	synbiotic	145:153	arg1	cheese					155:160	synbiotic cheese	145:160	synbiotic cheese	145:160	The current study aimed to produce synbiotic cheese, adding inulin and Bifidobacterium animalis subsp.
36014377	0	7	from	Effect	0:5	arg1	Acids					28:32	Organic Acids	20:32	Organic Acids	20:32	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	0	7	from	Effect	0:5	arg1	Microstructure					38:51	Microstructure	38:51	Microstructure of Synbiotic Cheddar-Type Cheese	38:84	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	3	8	theme	probiotic	354:362	arg1	count					364:368	probiotic count	354:368	probiotic count	354:368	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	4	9	theme	≤	456:456	arg1	p					454:454	p ≤ 0.01	454:461	p ≤ 0.01	454:461	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	4	9	theme	≤	456:456	arg1	inulin					446:451	inulin	446:451	inulin (p ≤ 0.01)	446:462	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	6	10	theme	cheese	757:762	arg1	likeliness					739:748	the likeliness	735:748	the likeliness of the cheese	735:762	Meanwhile, inulin increased the likeliness of the cheese, and its probiotic viability remained above 107 colony forming unit (CFU) per gram during ripening.
36014377	5	11	theme	cheese	689:694	arg1	structure					696:704	the cheese structure	685:704	the cheese structure	685:704	Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure.
36014377	0	12	theme	Buffalo	96:102	arg1	Milk					104:107	Buffalo Milk	96:107	Buffalo Milk	96:107	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	3	13	theme	cheese	377:382	arg1	count					364:368	probiotic count	354:368	probiotic count	354:368	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	3	13	theme	cheese	377:382	arg1	analysis					284:291	The physicochemical analysis	264:291	The physicochemical analysis	264:291	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	3	13	theme	cheese	377:382	arg1	content					321:327	minerals and organic acids content	294:327	minerals and organic acids content	294:327	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	3	13	theme	cheese	377:382	arg1	evaluation					338:347	sensory evaluation	330:347	sensory evaluation	330:347	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	5	14	theme	Scanning	601:608	arg1	microscopy					619:628	Scanning electron microscopy	601:628	Scanning electron microscopy (SEM) of the cheese	601:648	Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure.
36014377	5	14	theme	Scanning	601:608	arg1	SEM					631:633	SEM	631:633	SEM	631:633	Scanning electron microscopy (SEM) of the cheese revealed that inulin could improve the cheese structure.
36014377	1	15	theme	inulin	170:175	arg1	subsp					206:210	inulin and Bifidobacterium animalis subsp	170:210	subsp	206:210	The current study aimed to produce synbiotic cheese, adding inulin and Bifidobacterium animalis subsp.
36014377	3	16	theme	physicochemical	268:282	arg1	analysis					284:291	The physicochemical analysis	264:291	The physicochemical analysis	264:291	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	0	17	theme	Inulin	10:15	arg1	Effect					0:5	Effect	0:5	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese	0:84	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	1	18	theme	Bifidobacterium	181:195	arg1	subsp					206:210	inulin and Bifidobacterium animalis subsp	170:210	subsp	206:210	The current study aimed to produce synbiotic cheese, adding inulin and Bifidobacterium animalis subsp.
36014377	4	19	theme	physiochemical	517:530	arg1	composition					532:542	physiochemical composition	517:542	physiochemical composition	517:542	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	1	20	theme	animalis	197:204	arg1	subsp					206:210	inulin and Bifidobacterium animalis subsp	170:210	subsp	206:210	The current study aimed to produce synbiotic cheese, adding inulin and Bifidobacterium animalis subsp.
36014377	0	21	theme	Organic	20:26	arg1	Acids					28:32	Organic Acids	20:32	Organic Acids	20:32	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	4	22	theme	ripening	485:492	arg1	period					494:499	the ripening period	481:499	the ripening period	481:499	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	4	23	theme	inulin	446:451	arg1	effect					436:441	The significant effect	420:441	The significant effect of inulin (p ≤ 0.01)	420:462	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	3	24	theme	organic	307:313	arg1	acids					315:319	organic acids	307:319	organic acids	307:319	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	1	25	theme	current	114:120	arg1	study					122:126	The current study	110:126	The current study	110:126	The current study aimed to produce synbiotic cheese, adding inulin and Bifidobacterium animalis subsp.
36014377	4	26	theme	acid	567:570	arg1	contents					572:579	organic acid contents	559:579	organic acid contents	559:579	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	3	27	theme	minerals	294:301	arg1	content					321:327	minerals and organic acids content	294:327	minerals and organic acids content	294:327	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	4	28	theme	significant	424:434	arg1	effect					436:441	The significant effect	420:441	The significant effect of inulin (p ≤ 0.01)	420:462	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	4	29	theme	organic	559:565	arg1	contents					572:579	organic acid contents	559:579	organic acid contents	559:579	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	0	30	theme	Synbiotic	56:64	arg1	Cheese					79:84	Synbiotic Cheddar-Type Cheese	56:84	Synbiotic Cheddar-Type Cheese	56:84	Effect of Inulin on Organic Acids and Microstructure of Synbiotic Cheddar-Type Cheese Made from Buffalo Milk.
36014377	3	31	theme	sensory	330:336	arg1	evaluation					338:347	sensory evaluation	330:347	sensory evaluation	330:347	The physicochemical analysis, minerals and organic acids content, sensory evaluation, and probiotic count of the cheese were performed during the ripening.
36014377	4	32	from	changes	506:512	arg1	contents					572:579	organic acid contents	559:579	organic acid contents	559:579	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	4	32	from	changes	506:512	arg1	minerals					545:552	minerals	545:552	minerals	545:552	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
36014377	4	32	from	changes	506:512	arg1	composition					532:542	physiochemical composition	517:542	physiochemical composition	517:542	The significant effect of inulin (p ≤ 0.01) was found during the ripening period, and changes in physiochemical composition, minerals, and organic acid contents were also observed.
37052727	0	0	theme	endosperm	72:80	arg1	starch					82:87	endosperm starch	72:87	endosperm starch	72:87	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	1	1	theme	endosperm	260:268	arg1	pyrophosphorylase					282:298	endosperm ADP-glucose pyrophosphorylase	260:298	endosperm ADP-glucose pyrophosphorylase	260:298	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	0	2	dep	mays	103:106	arg1	L					108:108	L	108:108	Zea mays L.	99:109	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	9	3	theme	maize	1632:1636	arg1	content					1645:1651	maize starch content	1632:1651	maize starch content	1632:1651	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	1	4	theme	ADP-glucose	270:280	arg1	pyrophosphorylase					282:298	endosperm ADP-glucose pyrophosphorylase	260:298	endosperm ADP-glucose pyrophosphorylase	260:298	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	4	5	theme	transgenic	581:590	arg1	plants					592:597	transgenic plants	581:597	transgenic plants	581:597	In this study, ZmSUS1 was transformed into maize inbred line KN5585, and transgenic plants were obtained.
37052727	7	6	theme	amylose	1195:1201	arg1	content					1203:1209	the amylose content	1191:1209	the amylose content	1191:1209	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	9	7	theme	technology	1598:1607	arg1	strategy					1609:1616	a new technology strategy	1592:1616	a new technology strategy for improving maize starch content and yield	1592:1661	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	1	8	theme	pyrophosphorylase	282:298	arg1	Shrunken2					191:199	Shrunken2	191:199	Shrunken2 (Sh2)	191:205	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	8	theme	pyrophosphorylase	282:298	arg1	Brittle2					211:218	Brittle2	211:218	Brittle2 (Bt2)	211:224	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	8	theme	pyrophosphorylase	282:298	arg1	subunits					248:255	the size subunits	239:255	the size subunits of endosperm ADP-glucose pyrophosphorylase	239:298	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	8	9	theme	synthase1	1460:1468	arg1	synthase					1496:1503	starch synthase	1489:1503	starch synthase	1489:1503	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	8	9	theme	synthase1	1460:1468	arg1	expression					1396:1405	the expression	1392:1405	the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase	1392:1503	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	6	10	theme	maize	1066:1070	arg1	seeds					1072:1076	maize seeds	1066:1076	maize seeds	1066:1076	These results indicated that overexpression of ZmSUS1 can significantly improve the traits of maize seeds and obtain new lines with high amylose content.
37052727	6	11	theme	ZmSUS1	1019:1024	arg1	overexpression					1001:1014	overexpression	1001:1014	overexpression of ZmSUS1	1001:1024	These results indicated that overexpression of ZmSUS1 can significantly improve the traits of maize seeds and obtain new lines with high amylose content.
37052727	9	12	from	role	1538:1541	arg1	synthesis					1569:1577	maize starch synthesis	1556:1577	maize starch synthesis	1556:1577	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	0	13	theme	starch	82:87	arg1	composition					57:67	composition	57:67	composition	57:67	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	0	13	theme	starch	82:87	arg1	content					45:51	content	45:51	content	45:51	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	0	14	from	content	45:51	arg1	maize					92:96	maize	92:96	maize (Zea mays L.)	92:110	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	0	14	from	content	45:51	arg1	mays					103:106	Zea mays L.	99:109	Zea mays L.	99:109	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	9	15	theme	new	1594:1596	arg1	strategy					1609:1616	a new technology strategy	1592:1616	a new technology strategy for improving maize starch content and yield	1592:1661	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	1	16	theme	Brittle2	211:218	arg1	GBSS1					336:340	GBSS1	336:340	GBSS1	336:340	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	16	theme	Brittle2	211:218	arg1	SS1					365:367	SS1	365:367	SS1	365:367	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	16	theme	Brittle2	211:218	arg1	expression					177:186	the expression	173:186	the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase	173:298	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	16	theme	Brittle2	211:218	arg1	synthase1					354:362	Starch synthase1	347:362	Starch synthase1 (SS1)	347:368	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	16	theme	Brittle2	211:218	arg1	starchsynthase1					319:333	Granule bound starchsynthase1	305:333	Granule bound starchsynthase1 (GBSS1)	305:341	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	5	17	theme	non-transgenic	632:645	arg1	control					656:662	the non-transgenic negative control	628:662	the non-transgenic negative control	628:662	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	8	18	theme	ZmSUS1	1371:1376	arg1	expression					1357:1366	the ectopic expression	1345:1366	the ectopic expression of ZmSUS1	1345:1376	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	7	19	theme	ADP-glucose	1251:1261	arg1	subunits					1290:1297	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	19	theme	ADP-glucose	1251:1261	arg1	Brittle2					1319:1326	Brittle2	1319:1326	Brittle2 (Bt2)	1319:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	19	theme	ADP-glucose	1251:1261	arg1	Shrunken2					1299:1307	Shrunken2	1299:1307	Shrunken2 (Sh2)	1299:1313	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	4	20	theme	inbred	557:562	arg1	KN5585					569:574	maize inbred line KN5585	551:574	maize inbred line KN5585	551:574	In this study, ZmSUS1 was transformed into maize inbred line KN5585, and transgenic plants were obtained.
37052727	5	21	theme	negative	647:654	arg1	control					656:662	the non-transgenic negative control	628:662	the non-transgenic negative control	628:662	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	1	22	theme	Granule	305:311	arg1	GBSS1					336:340	GBSS1	336:340	GBSS1	336:340	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	22	theme	Granule	305:311	arg1	starchsynthase1					319:333	Granule bound starchsynthase1	305:333	Granule bound starchsynthase1 (GBSS1)	305:341	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	23	theme	amylose	134:140	arg1	content					142:148	the amylose content	130:148	the amylose content of maize	130:157	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	2	24	theme	Cereal	371:376	arg1	crops					378:382	Cereal crops	371:382	Cereal crops	371:382	Cereal crops accumulate starch in seeds as an energy reserve.
37052727	1	25	theme	bound	313:317	arg1	GBSS1					336:340	GBSS1	336:340	GBSS1	336:340	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	25	theme	bound	313:317	arg1	starchsynthase1					319:333	Granule bound starchsynthase1	305:333	Granule bound starchsynthase1 (GBSS1)	305:341	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	5	26	theme	starch	819:824	arg1	content					826:832	the total starch content	809:832	the total starch content	809:832	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	8	27	theme	Starch	1453:1458	arg1	SS1					1471:1473	SS1	1471:1473	SS1	1471:1473	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	8	27	theme	Starch	1453:1458	arg1	synthase1					1460:1468	Starch synthase1	1453:1468	Starch synthase1 (SS1)	1453:1474	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	9	28	theme	starch	1638:1643	arg1	content					1645:1651	maize starch content	1632:1651	maize starch content	1632:1651	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	5	29	theme	mature	751:756	arg1	seeds					758:762	mature seeds	751:762	mature seeds of transgenic maize	751:782	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	7	30	theme	subunits	1290:1297	arg1	expression					1227:1236	the expression	1223:1236	the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1223:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	4	31	theme	line	564:567	arg1	KN5585					569:574	maize inbred line KN5585	551:574	maize inbred line KN5585	551:574	In this study, ZmSUS1 was transformed into maize inbred line KN5585, and transgenic plants were obtained.
37052727	0	32	theme	ZmSUS1	22:27	arg1	gene					29:32	the ZmSUS1 gene	18:32	the ZmSUS1 gene	18:32	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	9	33	theme	important	1528:1536	arg1	role					1538:1541	the important role	1524:1541	the important role of ZmSUS1 in maize starch synthesis	1524:1577	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	7	34	theme	endosperm	1241:1249	arg1	subunits					1290:1297	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	34	theme	endosperm	1241:1249	arg1	Brittle2					1319:1326	Brittle2	1319:1326	Brittle2 (Bt2)	1319:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	34	theme	endosperm	1241:1249	arg1	Shrunken2					1299:1307	Shrunken2	1299:1307	Shrunken2 (Sh2)	1299:1313	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	6	35	theme	amylose	1109:1115	arg1	content					1117:1123	high amylose content	1104:1123	high amylose content	1104:1123	These results indicated that overexpression of ZmSUS1 can significantly improve the traits of maize seeds and obtain new lines with high amylose content.
37052727	1	36	theme	maize	153:157	arg1	content					142:148	the amylose content	130:148	the amylose content of maize	130:157	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	8	37	theme	bound	1418:1422	arg1	synthase1					1431:1439	Granule bound starch synthase1	1410:1439	Granule bound starch synthase1 (GBSS1)	1410:1447	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	8	37	theme	bound	1418:1422	arg1	GBSS1					1442:1446	GBSS1	1442:1446	GBSS1	1442:1446	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	0	38	theme	Zea	99:101	arg1	maize					92:96	maize	92:96	maize (Zea mays L.)	92:110	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	0	38	theme	Zea	99:101	arg1	mays					103:106	Zea mays L.	99:109	Zea mays L.	99:109	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	5	39	theme	granules	938:945	arg1	diameter					919:926	the average diameter	907:926	the average diameter of starch granules	907:945	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	3	40	theme	important	466:474	arg1	role					476:479	an important role	463:479	an important role	463:479	Sucrose Synthase (SuSy) plays an important role in grain starch synthesis.
37052727	5	41	theme	amylose	732:738	arg1	content					740:746	the amylose content	728:746	the amylose content in mature seeds of transgenic maize	728:782	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	8	42	theme	Granule	1410:1416	arg1	synthase1					1431:1439	Granule bound starch synthase1	1410:1439	Granule bound starch synthase1 (GBSS1)	1410:1447	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	8	42	theme	Granule	1410:1416	arg1	GBSS1					1442:1446	GBSS1	1442:1446	GBSS1	1442:1446	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	0	43	dep	content	45:51	arg1	the					41:43	the	41:43	the	41:43	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	5	44	theme	transgenic	767:776	arg1	maize					778:782	transgenic maize	767:782	transgenic maize	767:782	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	0	45	theme	gene	29:32	arg1	Overexpression					0:13	Overexpression	0:13	Overexpression of the ZmSUS1 gene	0:32	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	8	46	theme	synthase1	1431:1439	arg1	synthase					1496:1503	starch synthase	1489:1503	starch synthase	1489:1503	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	8	46	theme	synthase1	1431:1439	arg1	expression					1396:1405	the expression	1392:1405	the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase	1392:1503	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	9	47	theme	ZmSUS1	1546:1551	arg1	role					1538:1541	the important role	1524:1541	the important role of ZmSUS1 in maize starch synthesis	1524:1577	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	0	48	from	composition	57:67	arg1	maize					92:96	maize	92:96	maize (Zea mays L.)	92:110	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	0	48	from	composition	57:67	arg1	mays					103:106	Zea mays L.	99:109	Zea mays L.	99:109	Overexpression of the ZmSUS1 gene alters the content and composition of endosperm starch in maize (Zea mays L.).
37052727	6	49	theme	high	1104:1107	arg1	content					1117:1123	high amylose content	1104:1123	high amylose content	1104:1123	These results indicated that overexpression of ZmSUS1 can significantly improve the traits of maize seeds and obtain new lines with high amylose content.
37052727	5	50	from	content	740:746	arg1	seeds					758:762	mature seeds	751:762	mature seeds of transgenic maize	751:782	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	5	51	theme	maize	778:782	arg1	seeds					758:762	mature seeds	751:762	mature seeds of transgenic maize	751:782	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	3	52	theme	grain	484:488	arg1	synthesis					497:505	grain starch synthesis	484:505	grain starch synthesis	484:505	Sucrose Synthase (SuSy) plays an important role in grain starch synthesis.
37052727	5	53	theme	SuSy	693:696	arg1	activity					681:688	activity	681:688	activity	681:688	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	5	53	theme	SuSy	693:696	arg1	content					669:675	the content	665:675	the content	665:675	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	1	54	theme	size	243:246	arg1	Shrunken2					191:199	Shrunken2	191:199	Shrunken2 (Sh2)	191:205	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	54	theme	size	243:246	arg1	Brittle2					211:218	Brittle2	211:218	Brittle2 (Bt2)	211:224	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	54	theme	size	243:246	arg1	subunits					248:255	the size subunits	239:255	the size subunits of endosperm ADP-glucose pyrophosphorylase	239:298	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	9	55	theme	maize	1556:1560	arg1	synthesis					1569:1577	maize starch synthesis	1556:1577	maize starch synthesis	1556:1577	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	5	56	theme	average	911:917	arg1	diameter					919:926	the average diameter	907:926	the average diameter of starch granules	907:945	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	3	57	theme	starch	490:495	arg1	synthesis					497:505	grain starch synthesis	484:505	grain starch synthesis	484:505	Sucrose Synthase (SuSy) plays an important role in grain starch synthesis.
37052727	8	58	theme	starch	1489:1494	arg1	synthase					1496:1503	starch synthase	1489:1503	starch synthase	1489:1503	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	8	58	theme	starch	1489:1494	arg1	expression					1396:1405	the expression	1392:1405	the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase	1392:1503	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	9	59	theme	starch	1562:1567	arg1	synthesis					1569:1577	maize starch synthesis	1556:1577	maize starch synthesis	1556:1577	This study proved the important role of ZmSUS1 in maize starch synthesis and provided a new technology strategy for improving maize starch content and yield.
37052727	1	60	theme	Starch	347:352	arg1	SS1					365:367	SS1	365:367	SS1	365:367	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	60	theme	Starch	347:352	arg1	synthase1					354:362	Starch synthase1	347:362	Starch synthase1 (SS1)	347:368	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	5	61	theme	100-grain	862:870	arg1	weight					872:877	the 100-grain weight	858:877	the 100-grain weight	858:877	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	7	62	theme	pyrophosphorylase	1263:1279	arg1	subunits					1290:1297	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	62	theme	pyrophosphorylase	1263:1279	arg1	Brittle2					1319:1326	Brittle2	1319:1326	Brittle2 (Bt2)	1319:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	62	theme	pyrophosphorylase	1263:1279	arg1	Shrunken2					1299:1307	Shrunken2	1299:1307	Shrunken2 (Sh2)	1299:1313	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	63	theme	AGPase	1282:1287	arg1	subunits					1290:1297	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	63	theme	AGPase	1282:1287	arg1	Brittle2					1319:1326	Brittle2	1319:1326	Brittle2 (Bt2)	1319:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	63	theme	AGPase	1282:1287	arg1	Shrunken2					1299:1307	Shrunken2	1299:1307	Shrunken2 (Sh2)	1299:1313	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	5	64	theme	total	813:817	arg1	content					826:832	the total starch content	809:832	the total starch content	809:832	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	6	65	with	lines	1093:1097	arg1	content					1117:1123	high amylose content	1104:1123	high amylose content	1104:1123	These results indicated that overexpression of ZmSUS1 can significantly improve the traits of maize seeds and obtain new lines with high amylose content.
37052727	2	66	theme	energy	417:422	arg1	reserve					424:430	an energy reserve	414:430	an energy reserve	414:430	Cereal crops accumulate starch in seeds as an energy reserve.
37052727	5	67	theme	starch	931:936	arg1	granules					938:945	starch granules	931:945	starch granules	931:945	Compared with the non-transgenic negative control, the content and activity of SuSy were significantly increased, the amylose content in mature seeds of transgenic maize increased by 41.1-69.2%, the total starch content increased by 5.0-13.5%, the 100-grain weight increased by 19.0-26.2% and the average diameter of starch granules increased by 10.8-17.2%.
37052727	7	68	dep	subunits	1290:1297	arg1	subunits					1290:1297	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2)	1241:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	68	dep	subunits	1290:1297	arg1	Bt2					1329:1331	Bt2	1329:1331	Bt2	1329:1331	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	68	dep	subunits	1290:1297	arg1	Sh2					1310:1312	Sh2	1310:1312	Sh2	1310:1312	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	68	dep	subunits	1290:1297	arg1	Brittle2					1319:1326	Brittle2	1319:1326	Brittle2 (Bt2)	1319:1332	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	68	dep	subunits	1290:1297	arg1	Shrunken2					1299:1307	Shrunken2	1299:1307	Shrunken2 (Sh2)	1299:1313	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	7	69	theme	ZmSUS1	1171:1176	arg1	overexpression					1153:1166	the overexpression	1149:1166	the overexpression of ZmSUS1	1149:1176	It was also found that the overexpression of ZmSUS1 may increase the amylose content by altering the expression of endosperm ADP-glucose pyrophosphorylase (AGPase) subunits Shrunken2 (Sh2) and Brittle2 (Bt2).
37052727	3	70	theme	Sucrose	433:439	arg1	SuSy					451:454	SuSy	451:454	SuSy	451:454	Sucrose Synthase (SuSy) plays an important role in grain starch synthesis.
37052727	3	70	theme	Sucrose	433:439	arg1	Synthase					441:448	Sucrose Synthase	433:448	Sucrose Synthase (SuSy)	433:455	Sucrose Synthase (SuSy) plays an important role in grain starch synthesis.
37052727	4	71	theme	maize	551:555	arg1	KN5585					569:574	maize inbred line KN5585	551:574	maize inbred line KN5585	551:574	In this study, ZmSUS1 was transformed into maize inbred line KN5585, and transgenic plants were obtained.
37052727	1	72	theme	Shrunken2	191:199	arg1	GBSS1					336:340	GBSS1	336:340	GBSS1	336:340	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	72	theme	Shrunken2	191:199	arg1	SS1					365:367	SS1	365:367	SS1	365:367	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	72	theme	Shrunken2	191:199	arg1	expression					177:186	the expression	173:186	the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase	173:298	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	72	theme	Shrunken2	191:199	arg1	synthase1					354:362	Starch synthase1	347:362	Starch synthase1 (SS1)	347:368	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	1	72	theme	Shrunken2	191:199	arg1	starchsynthase1					319:333	Granule bound starchsynthase1	305:333	Granule bound starchsynthase1 (GBSS1)	305:341	ZmSUS1 increases the amylose content of maize by regulating the expression of Shrunken2 (Sh2) and Brittle2 (Bt2) which encode the size subunits of endosperm ADP-glucose pyrophosphorylase, and Granule bound starchsynthase1 (GBSS1) and Starch synthase1 (SS1).
37052727	8	73	theme	ectopic	1349:1355	arg1	expression					1357:1366	the ectopic expression	1345:1366	the ectopic expression of ZmSUS1	1345:1376	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	6	74	theme	seeds	1072:1076	arg1	traits					1056:1061	the traits	1052:1061	the traits of maize seeds	1052:1076	These results indicated that overexpression of ZmSUS1 can significantly improve the traits of maize seeds and obtain new lines with high amylose content.
37052727	6	75	theme	new	1089:1091	arg1	lines					1093:1097	new lines	1089:1097	new lines with high amylose content	1089:1123	These results indicated that overexpression of ZmSUS1 can significantly improve the traits of maize seeds and obtain new lines with high amylose content.
37052727	8	76	theme	starch	1424:1429	arg1	synthase1					1431:1439	Granule bound starch synthase1	1410:1439	Granule bound starch synthase1 (GBSS1)	1410:1447	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37052727	8	76	theme	starch	1424:1429	arg1	GBSS1					1442:1446	GBSS1	1442:1446	GBSS1	1442:1446	Moreover, the ectopic expression of ZmSUS1 also affected the expression of Granule bound starch synthase1 (GBSS1) and Starch synthase1 (SS1) which encode starch synthase.
37329602	5	0	theme	FTIR	815:818	arg1	analysis					820:827	FTIR analysis	815:827	FTIR analysis	815:827	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	9	1	theme	magnetic	1412:1419	arg1	NPs					1421:1423	surface-functionalized magnetic NPs	1389:1423	surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy	1389:1517	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	5	2	theme	OH	717:718	arg1	NPs					792:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	3	3	theme	chitosan-coated	582:596	arg1	NPs					604:606	chitosan-coated Mn3O4 NPs	582:606	chitosan-coated Mn3O4 NPs	582:606	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	4	4	theme	EDX	670:672	arg1	analysis					674:681	EDX analysis	670:681	EDX analysis	670:681	Elemental composition in the prepared sample was examined by EDX analysis.
37329602	8	5	theme	cell	1246:1249	arg1	line					1251:1254	the MCF-7 cancer cell line	1229:1254	the MCF-7 cancer cell line	1229:1254	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	8	6	theme	MTT	1176:1178	arg1	assay					1180:1184	The MTT assay	1172:1184	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line	1172:1254	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	5	7	theme	Various	684:690	arg1	NPs					792:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	5	7	theme	Various	684:690	arg1	modes					692:696	Various modes	684:696	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	684:794	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	1	8	theme	biomedical	221:230	arg1	sciences					232:239	biomedical sciences	221:239	biomedical sciences	221:239	Biocompatible polymer-functionalized magnetic nanoparticles could offer promising applications in biomedical sciences.
37329602	7	9	theme	antimicrobial	1146:1158	arg1	activities					1160:1169	clear antimicrobial activities	1140:1169	clear antimicrobial activities	1140:1169	In-vitro bioassay, antibacterial activity was tested against Escherichiacoli, Bacillus cereus, and anti-fungal activities against two Fusarium strains indicated clear antimicrobial activities.
37329602	2	10	theme	octahedral	367:376	arg1	structure					386:394	an octahedral crystal structure	364:394	an octahedral crystal structure having a crystallite size of 10-17 nm	364:432	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	11	theme	crystal	378:384	arg1	structure					386:394	an octahedral crystal structure	364:394	an octahedral crystal structure having a crystallite size of 10-17 nm	364:432	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	5	12	theme	Mn3O4	786:790	arg1	spectrum					774:781	the spectrum	770:781	the spectrum of Mn3O4	770:790	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	9	13	theme	cancer	1451:1456	arg1	treatment					1438:1446	the treatment	1434:1446	the treatment of cancer	1434:1456	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	7	14	dep	bioassay	988:995	arg1	In-vitro					979:986	In-vitro	979:986	In-vitro	979:986	In-vitro bioassay, antibacterial activity was tested against Escherichiacoli, Bacillus cereus, and anti-fungal activities against two Fusarium strains indicated clear antimicrobial activities.
37329602	9	15	theme	controlled	1476:1485	arg1	process					1487:1493	a remote controlled process	1467:1493	a remote controlled process of hyperthermia therapy	1467:1517	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	8	16	theme	MCF-7	1233:1237	arg1	line					1251:1254	the MCF-7 cancer cell line	1229:1254	the MCF-7 cancer cell line	1229:1254	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	2	17	theme	10-17 nm	425:432	arg1	size					417:420	a crystallite size	403:420	a crystallite size of 10-17 nm	403:432	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	5	18	attach	attached	758:765	arg2	CH2					754:756	CH2	754:756	CH2 attached to the spectrum of Mn3O4	754:790	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	5	18	attach	attached	758:765	arg1	spectrum					774:781	the spectrum	770:781	the spectrum of Mn3O4	770:790	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	8	19	dep	assay	1180:1184	arg1	examine					1189:1195	examine	1189:1195	to examine the anticancer activity against the MCF-7 cancer cell line	1186:1254	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	8	20	theme	PEG-coated	1318:1327	arg1	NPs					1335:1337	PEG-coated Mn3O4 NPs	1318:1337	PEG-coated Mn3O4 NPs	1318:1337	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	9	21	dep	propose	1376:1382	arg1	NPs					1421:1423	surface-functionalized magnetic NPs	1389:1423	surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy	1389:1517	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	3	22	theme	SEM	471:473	arg1	graph					475:479	The SEM graph	467:479	The SEM graph	467:479	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	8	23	theme	MRI	1281:1283	arg1	agent					1294:1298	the T1 MRI contrast agent	1274:1298	the T1 MRI contrast agent	1274:1298	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	5	24	theme	MnOH	711:714	arg1	NPs					792:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	6	25	theme	magnetization	834:846	arg1	factor					848:853	The magnetization factor	830:853	The magnetization factor	830:853	The magnetization factor decreased and increase the coreacivity and retentivity of surface functionalized Mn3O4-NPs was calculated via VSM analysis.
37329602	6	26	theme	VSM	965:967	arg1	analysis					969:976	VSM analysis	965:976	VSM analysis	965:976	The magnetization factor decreased and increase the coreacivity and retentivity of surface functionalized Mn3O4-NPs was calculated via VSM analysis.
37329602	0	27	theme	Polyethylene	0:11	arg1	glycol					13:18	Polyethylene glycol	0:18	Polyethylene glycol	0:18	Polyethylene glycol and chitosan functionalized manganese oxide nanoparticles for antimicrobial and anticancer activities.
37329602	8	28	theme	anticancer	1201:1210	arg1	activity					1212:1219	the anticancer activity	1197:1219	the anticancer activity against the MCF-7 cancer cell line	1197:1254	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	7	29	theme	antibacterial	998:1010	arg1	activity					1012:1019	antibacterial activity	998:1019	antibacterial activity	998:1019	In-vitro bioassay, antibacterial activity was tested against Escherichiacoli, Bacillus cereus, and anti-fungal activities against two Fusarium strains indicated clear antimicrobial activities.
37329602	3	30	theme	smooth	510:515	arg1	surface					517:523	the non-uniform and smooth surface	490:523	the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs	490:555	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	4	31	theme	prepared	638:645	arg1	sample					647:652	the prepared sample	634:652	the prepared sample	634:652	Elemental composition in the prepared sample was examined by EDX analysis.
37329602	1	32	theme	Biocompatible	123:135	arg1	nanoparticles					169:181	Biocompatible polymer-functionalized magnetic nanoparticles	123:181	Biocompatible polymer-functionalized magnetic nanoparticles	123:181	Biocompatible polymer-functionalized magnetic nanoparticles could offer promising applications in biomedical sciences.
37329602	3	33	theme	NPs	553:555	arg1	surface					517:523	the non-uniform and smooth surface	490:523	the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs	490:555	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	8	34	theme	contrast	1285:1292	arg1	agent					1294:1298	the T1 MRI contrast agent	1274:1298	the T1 MRI contrast agent	1274:1298	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	1	35	theme	polymer-functionalized	137:158	arg1	nanoparticles					169:181	Biocompatible polymer-functionalized magnetic nanoparticles	123:181	Biocompatible polymer-functionalized magnetic nanoparticles	123:181	Biocompatible polymer-functionalized magnetic nanoparticles could offer promising applications in biomedical sciences.
37329602	1	36	theme	promising	195:203	arg1	applications					205:216	promising applications	195:216	promising applications	195:216	Biocompatible polymer-functionalized magnetic nanoparticles could offer promising applications in biomedical sciences.
37329602	0	37	theme	manganese	48:56	arg1	nanoparticles					64:76	manganese oxide nanoparticles	48:76	manganese oxide nanoparticles for antimicrobial and anticancer activities	48:120	Polyethylene glycol and chitosan functionalized manganese oxide nanoparticles for antimicrobial and anticancer activities.
37329602	6	38	theme	Mn3O4-NPs	936:944	arg1	retentivity					898:908	retentivity	898:908	retentivity of surface functionalized Mn3O4-NPs	898:944	The magnetization factor decreased and increase the coreacivity and retentivity of surface functionalized Mn3O4-NPs was calculated via VSM analysis.
37329602	8	39	theme	anti-cancer	1349:1359	arg1	activities					1361:1370	anti-cancer activities	1349:1370	anti-cancer activities	1349:1370	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	3	40	theme	PEG-functionalized	528:545	arg1	NPs					553:555	PEG-functionalized Mn3O4 NPs	528:555	PEG-functionalized Mn3O4 NPs	528:555	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	3	41	theme	non-uniform	494:504	arg1	surface					517:523	the non-uniform and smooth surface	490:523	the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs	490:555	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	9	42	theme	remote	1469:1474	arg1	process					1487:1493	a remote controlled process	1467:1493	a remote controlled process of hyperthermia therapy	1467:1517	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	6	43	theme	functionalized	921:934	arg1	Mn3O4-NPs					936:944	surface functionalized Mn3O4-NPs	913:944	surface functionalized Mn3O4-NPs	913:944	The magnetization factor decreased and increase the coreacivity and retentivity of surface functionalized Mn3O4-NPs was calculated via VSM analysis.
37329602	3	44	theme	Mn3O4	547:551	arg1	NPs					553:555	PEG-functionalized Mn3O4 NPs	528:555	PEG-functionalized Mn3O4 NPs	528:555	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	4	45	from	composition	619:629	arg1	sample					647:652	the prepared sample	634:652	the prepared sample	634:652	Elemental composition in the prepared sample was examined by EDX analysis.
37329602	2	46	with	nanoparticles	313:325	arg1	method					353:358	the co-precipitation method	332:358	the co-precipitation method	332:358	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	7	47	theme	clear	1140:1144	arg1	activities					1160:1169	clear antimicrobial activities	1140:1169	clear antimicrobial activities	1140:1169	In-vitro bioassay, antibacterial activity was tested against Escherichiacoli, Bacillus cereus, and anti-fungal activities against two Fusarium strains indicated clear antimicrobial activities.
37329602	6	48	theme	surface	913:919	arg1	Mn3O4-NPs					936:944	surface functionalized Mn3O4-NPs	913:944	surface functionalized Mn3O4-NPs	913:944	The magnetization factor decreased and increase the coreacivity and retentivity of surface functionalized Mn3O4-NPs was calculated via VSM analysis.
37329602	7	49	theme	Fusarium	1113:1120	arg1	strains					1122:1128	two Fusarium strains	1109:1128	two Fusarium strains	1109:1128	In-vitro bioassay, antibacterial activity was tested against Escherichiacoli, Bacillus cereus, and anti-fungal activities against two Fusarium strains indicated clear antimicrobial activities.
37329602	1	50	theme	magnetic	160:167	arg1	nanoparticles					169:181	Biocompatible polymer-functionalized magnetic nanoparticles	123:181	Biocompatible polymer-functionalized magnetic nanoparticles	123:181	Biocompatible polymer-functionalized magnetic nanoparticles could offer promising applications in biomedical sciences.
37329602	0	51	theme	oxide	58:62	arg1	nanoparticles					64:76	manganese oxide nanoparticles	48:76	manganese oxide nanoparticles for antimicrobial and anticancer activities	48:120	Polyethylene glycol and chitosan functionalized manganese oxide nanoparticles for antimicrobial and anticancer activities.
37329602	2	52	contain	having	396:401	arg1	structure					386:394	an octahedral crystal structure	364:394	an octahedral crystal structure having a crystallite size of 10-17 nm	364:432	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	52	contain	having	396:401	arg2	size					417:420	a crystallite size	403:420	a crystallite size of 10-17 nm	403:432	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	5	53	theme	anti-symmetric	736:749	arg1	NPs					792:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs	706:794	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	2	54	theme	tetra	293:297	arg1	Mn3O4					306:310	Mn3O4	306:310	Mn3O4	306:310	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	54	theme	tetra	293:297	arg1	oxide					299:303	polymer functionalized tri-manganese tetra oxide	256:303	polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method	256:358	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	55	theme	oxide	299:303	arg1	nanoparticles					313:325	polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles	256:325	polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method	256:358	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	3	56	theme	Mn3O4	598:602	arg1	NPs					604:606	chitosan-coated Mn3O4 NPs	582:606	chitosan-coated Mn3O4 NPs	582:606	The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn3O4 NPs as compared to Mn3O4 and chitosan-coated Mn3O4 NPs.
37329602	2	57	theme	tri-manganese	279:291	arg1	Mn3O4					306:310	Mn3O4	306:310	Mn3O4	306:310	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	57	theme	tri-manganese	279:291	arg1	oxide					299:303	polymer functionalized tri-manganese tetra oxide	256:303	polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method	256:358	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	58	theme	co-precipitation	336:351	arg1	method					353:358	the co-precipitation method	332:358	the co-precipitation method	332:358	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	0	59	dep	antimicrobial	82:94	arg1	activities					111:120	activities	111:120	activities	111:120	Polyethylene glycol and chitosan functionalized manganese oxide nanoparticles for antimicrobial and anticancer activities.
37329602	9	60	theme	hyperthermia	1498:1509	arg1	therapy					1511:1517	hyperthermia therapy	1498:1517	hyperthermia therapy	1498:1517	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	5	61	theme	CH2	754:756	arg1	anti-symmetric					736:749	anti-symmetric	736:749	anti-symmetric	736:749	Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH2 attached to the spectrum of Mn3O4 NPs were observed with FTIR analysis.
37329602	2	62	theme	functionalized	264:277	arg1	Mn3O4					306:310	Mn3O4	306:310	Mn3O4	306:310	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	62	theme	functionalized	264:277	arg1	oxide					299:303	polymer functionalized tri-manganese tetra oxide	256:303	polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method	256:358	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	8	63	theme	T1	1278:1279	arg1	agent					1294:1298	the T1 MRI contrast agent	1274:1298	the T1 MRI contrast agent	1274:1298	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	2	64	theme	crystallite	405:415	arg1	size					417:420	a crystallite size	403:420	a crystallite size of 10-17 nm	403:432	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	9	65	theme	therapy	1511:1517	arg1	process					1487:1493	a remote controlled process	1467:1493	a remote controlled process of hyperthermia therapy	1467:1517	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	8	66	theme	cancer	1239:1244	arg1	line					1251:1254	the MCF-7 cancer cell line	1229:1254	the MCF-7 cancer cell line	1229:1254	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	2	67	theme	polymer	256:262	arg1	Mn3O4					306:310	Mn3O4	306:310	Mn3O4	306:310	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	2	67	theme	polymer	256:262	arg1	oxide					299:303	polymer functionalized tri-manganese tetra oxide	256:303	polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method	256:358	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	7	68	theme	anti-fungal	1078:1088	arg1	activities					1090:1099	anti-fungal activities	1078:1099	anti-fungal activities against two Fusarium strains	1078:1128	In-vitro bioassay, antibacterial activity was tested against Escherichiacoli, Bacillus cereus, and anti-fungal activities against two Fusarium strains indicated clear antimicrobial activities.
37329602	2	69	theme	XRD	453:455	arg1	analyses					457:464	XRD analyses	453:464	XRD analyses	453:464	We fabricated polymer functionalized tri-manganese tetra oxide (Mn3O4) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses.
37329602	8	70	theme	Mn3O4	1329:1333	arg1	NPs					1335:1337	PEG-coated Mn3O4 NPs	1318:1337	PEG-coated Mn3O4 NPs	1318:1337	The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T1 MRI contrast agent demonstrated that PEG-coated Mn3O4 NPs exhibited anti-cancer activities.
37329602	9	71	theme	surface-functionalized	1389:1410	arg1	NPs					1421:1423	surface-functionalized magnetic NPs	1389:1423	surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy	1389:1517	We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.
37329602	4	72	theme	Elemental	609:617	arg1	composition					619:629	Elemental composition	609:629	Elemental composition in the prepared sample	609:652	Elemental composition in the prepared sample was examined by EDX analysis.
36500449	7	0	theme	at	1162:1163	arg1	break					1165:1169	elongation at break	1151:1169	elongation at break	1151:1169	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	2	1	theme	antimicrobial	488:500	arg1	properties					502:511	antimicrobial properties	488:511	antimicrobial properties	488:511	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	1	theme	antimicrobial	488:500	arg1	mechanical					450:459	mechanical	450:459	mechanical	450:459	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	2	theme	alginate-based	326:339	arg1	films					341:345	alginate-based films	326:345	alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm)	326:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	7	3	from	break	1165:1169	arg1	at					1162:1163	elongation at break	1151:1169	elongation at break	1151:1169	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	7	3	from	break	1165:1169	arg1	elongation					1151:1160	elongation at break	1151:1169	elongation at break	1151:1169	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	2	4	theme	food	549:552	arg1	applications					564:575	food packaging applications	549:575	food packaging applications	549:575	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	5	from	bio-filler	312:321	arg1	films					341:345	alginate-based films	326:345	alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm)	326:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	5	from	bio-filler	312:321	arg1	concentrations					360:373	different concentrations	350:373	different concentrations (10, 30, and 50%, w/w alginate)	350:405	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	5	from	bio-filler	312:321	arg1	μm					439:440	100 and 200 μm	427:440	100 and 200 μm	427:440	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	5	from	bio-filler	312:321	arg1	sizes					420:424	particle sizes	411:424	particle sizes (100 and 200 μm)	411:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	6	theme	w/w	393:395	arg1	%					390:390	10, 30, and 50%	376:390	10, 30, and 50%	376:390	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	6	theme	w/w	393:395	arg1	alginate					397:404	w/w alginate	393:404	w/w alginate	393:404	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	7	theme	seaweed	292:298	arg1	powder					300:305	seaweed powder	292:305	seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm)	292:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	4	8	theme	positive	796:803	arg1	effect					805:810	any positive effect	792:810	any positive effect	792:810	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	4	9	theme	films	857:861	arg1	properties					830:839	the mechanical properties	815:839	the mechanical properties of the alginate films	815:861	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	10	10	theme	different	1841:1849	arg1	composition					1851:1861	different composition	1841:1861	different composition	1841:1861	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	10	11	theme	respective	1884:1893	arg1	walls					1900:1904	the respective cell walls	1880:1904	the respective cell walls	1880:1904	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	0	12	theme	Water	127:131	arg1	Effect					81:86	Effect	81:86	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.	0:132	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	10	13	theme	pronounced	1700:1709	arg1	effect					1722:1727	A more pronounced inhibitory effect	1693:1727	A more pronounced inhibitory effect	1693:1727	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	9	14	theme	highest	1629:1635	arg1	concentration					1637:1649	the highest concentration	1625:1649	the highest concentration of the film-forming solution (83.3% v/v)	1625:1690	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	3	15	theme	optimal	695:701	arg1	temperature					703:713	the optimal temperature	691:713	the optimal temperature for preparing alginate solution	691:745	Initially, mechanical properties of the alginate films prepared at different temperatures were evaluated to find the optimal temperature for preparing alginate solution.
36500449	4	16	contain	have	787:790	arg2	effect					805:810	any positive effect	792:810	any positive effect	792:810	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	4	16	contain	have	787:790	arg1	addition					752:759	The addition	748:759	The addition of seaweed powder	748:777	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	5	17	theme	seaweed	979:985	arg1	filler					987:992	seaweed filler	979:992	seaweed filler	979:992	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	11	18	theme	enhanced	1999:2006	arg1	functionality					2008:2020	enhanced functionality	1999:2020	enhanced functionality	1999:2020	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	9	19	theme	film-forming	1658:1669	arg1	v/v					1687:1689	83.3% v/v	1681:1689	83.3% v/v	1681:1689	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	9	19	theme	film-forming	1658:1669	arg1	solution					1671:1678	the film-forming solution	1654:1678	the film-forming solution (83.3% v/v)	1654:1690	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	7	20	theme	distilled	1246:1254	arg1	water					1256:1260	distilled water	1246:1260	distilled water	1246:1260	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	3	21	theme	alginate	729:736	arg1	solution					738:745	alginate solution	729:745	alginate solution	729:745	Initially, mechanical properties of the alginate films prepared at different temperatures were evaluated to find the optimal temperature for preparing alginate solution.
36500449	7	22	theme	elongation	1151:1160	arg1	break					1165:1169	elongation at break	1151:1169	elongation at break	1151:1169	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	9	23	from	concentration	1637:1649	arg1	pronounced					1554:1563	pronounced	1554:1563	pronounced	1554:1563	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	9	23	from	concentration	1637:1649	arg1	activity					1479:1486	The antimicrobial activity	1461:1486	The antimicrobial activity of the alginate-seaweed film-forming solution	1461:1532	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	8	24	theme	barrier	1395:1401	arg1	properties					1403:1412	improved barrier properties	1386:1412	improved barrier properties	1386:1412	The films prepared in PAW also showed improved barrier properties compared to those prepared in distilled water.
36500449	11	25	from	potential	1939:1947	arg1	combination					1970:1980	combination	1970:1980	combination with PAW	1970:1989	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	0	26	theme	Films	74:78	arg1	Properties					51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties	0:60	Properties	51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	1	27	theme	natural	155:161	arg1	fillers					163:169	natural fillers	155:169	natural fillers such as seaweed	155:185	The incorporation of natural fillers such as seaweed may potentially enhance the properties of biopolymer films.
36500449	1	27	theme	natural	155:161	arg1	seaweed					179:185	seaweed	179:185	seaweed	179:185	The incorporation of natural fillers such as seaweed may potentially enhance the properties of biopolymer films.
36500449	9	28	theme	%	1685:1685	arg1	v/v					1687:1689	83.3% v/v	1681:1689	83.3% v/v	1681:1689	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	9	28	theme	%	1685:1685	arg1	solution					1671:1678	the film-forming solution	1654:1678	the film-forming solution (83.3% v/v)	1654:1690	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	5	29	theme	vapor	892:896	arg1	barrier					877:883	the barrier	873:883	the barrier (water vapor transmission rate)	873:915	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	5	29	theme	vapor	892:896	arg1	rate					911:914	water vapor transmission rate	886:914	water vapor transmission rate	886:914	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	9	30	from	pronounced	1554:1563	arg1	general					1541:1547	general	1541:1547	general	1541:1547	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	2	31	theme	packaging	554:562	arg1	applications					564:575	food packaging applications	549:575	food packaging applications	549:575	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	0	32	theme	Seaweed	91:97	arg1	Powder					99:104	Seaweed Powder	91:104	Seaweed Powder	91:104	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	9	33	theme	antimicrobial	1465:1477	arg1	pronounced					1554:1563	pronounced	1554:1563	pronounced	1554:1563	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	9	33	theme	antimicrobial	1465:1477	arg1	activity					1479:1486	The antimicrobial activity	1461:1486	The antimicrobial activity of the alginate-seaweed film-forming solution	1461:1532	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	9	34	from	general	1541:1547	arg1	pronounced					1554:1563	pronounced	1554:1563	pronounced	1554:1563	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	9	34	from	general	1541:1547	arg1	activity					1479:1486	The antimicrobial activity	1461:1486	The antimicrobial activity of the alginate-seaweed film-forming solution	1461:1532	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	11	35	with	combination	1970:1980	arg1	PAW					1987:1989	PAW	1987:1989	PAW	1987:1989	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	11	36	theme	food	2070:2073	arg1	applications					2085:2096	potential food packaging applications	2060:2096	potential food packaging applications	2060:2096	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	10	37	dep	composition	1851:1861	arg1	the					1837:1839	the	1837:1839	the	1837:1839	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	0	38	theme	Mechanical	0:9	arg1	Properties					51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties	0:60	Properties	51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	11	39	theme	alginate	2041:2048	arg1	films					2050:2054	alginate films	2041:2054	alginate films	2041:2054	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	0	40	dep	Properties	51:60	arg1	Barrier					12:18	Mechanical, Barrier, Antioxidant and Antimicrobial Properties	0:60	Barrier	12:18	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	0	40	dep	Properties	51:60	arg1	Effect					81:86	Effect	81:86	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.	0:132	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	9	41	theme	alginate-seaweed	1495:1510	arg1	solution					1525:1532	the alginate-seaweed film-forming solution	1491:1532	the alginate-seaweed film-forming solution	1491:1532	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	7	42	theme	mechanical	1102:1111	arg1	properties					1113:1122	The mechanical properties	1098:1122	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW	1098:1201	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	0	43	theme	Antioxidant	21:31	arg1	Properties					51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties	0:60	Properties	51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	9	44	theme	solution	1525:1532	arg1	pronounced					1554:1563	pronounced	1554:1563	pronounced	1554:1563	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	9	44	theme	solution	1525:1532	arg1	activity					1479:1486	The antimicrobial activity	1461:1486	The antimicrobial activity of the alginate-seaweed film-forming solution	1461:1532	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	7	45	dep	properties	1113:1122	arg1	strength					1133:1140	tensile strength	1125:1140	tensile strength	1125:1140	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	7	45	dep	properties	1113:1122	arg1	break					1165:1169	elongation at break	1151:1169	elongation at break	1151:1169	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	4	46	theme	powder	772:777	arg1	addition					752:759	The addition	748:759	The addition of seaweed powder	748:777	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	0	47	theme	Antimicrobial	37:49	arg1	Properties					51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties	0:60	Properties	51:60	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	7	48	theme	films	1179:1183	arg1	properties					1113:1122	The mechanical properties	1098:1122	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW	1098:1201	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	3	49	theme	mechanical	589:598	arg1	properties					600:609	mechanical properties	589:609	mechanical properties of the alginate films prepared at different temperatures	589:666	Initially, mechanical properties of the alginate films prepared at different temperatures were evaluated to find the optimal temperature for preparing alginate solution.
36500449	2	50	theme	different	350:358	arg1	concentrations					360:373	different concentrations	350:373	different concentrations (10, 30, and 50%, w/w alginate)	350:405	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	1	51	theme	biopolymer	229:238	arg1	films					240:244	biopolymer films	229:244	biopolymer films	229:244	The incorporation of natural fillers such as seaweed may potentially enhance the properties of biopolymer films.
36500449	11	52	theme	seaweed	1952:1958	arg1	filler					1960:1965	seaweed filler	1952:1965	seaweed filler in combination with PAW	1952:1989	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	2	53	theme	alginate	516:523	arg1	barrier					462:468	barrier	462:468	barrier	462:468	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	53	theme	alginate	516:523	arg1	properties					502:511	antimicrobial properties	488:511	antimicrobial properties	488:511	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	53	theme	alginate	516:523	arg1	mechanical					450:459	mechanical	450:459	mechanical	450:459	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	53	theme	alginate	516:523	arg1	antioxidant					471:481	antioxidant	471:481	antioxidant	471:481	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	3	54	theme	alginate	618:625	arg1	films					627:631	the alginate films	614:631	the alginate films prepared at different temperatures	614:666	Initially, mechanical properties of the alginate films prepared at different temperatures were evaluated to find the optimal temperature for preparing alginate solution.
36500449	2	55	from	concentrations	360:373	arg1	bio-filler					312:321	a bio-filler	310:321	a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm)	310:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	55	from	concentrations	360:373	arg1	films					341:345	alginate-based films	326:345	alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm)	326:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	1	56	theme	fillers	163:169	arg1	incorporation					138:150	The incorporation	134:150	The incorporation of natural fillers such as seaweed	134:185	The incorporation of natural fillers such as seaweed may potentially enhance the properties of biopolymer films.
36500449	11	57	theme	films	2050:2054	arg1	functionality					2008:2020	enhanced functionality	1999:2020	enhanced functionality	1999:2020	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	11	57	theme	films	2050:2054	arg1	bioactivity					2026:2036	bioactivity	2026:2036	bioactivity of alginate films for potential food packaging applications	2026:2096	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	7	58	theme	seaweed	1332:1338	arg1	filler					1340:1345	the seaweed filler	1328:1345	the seaweed filler	1328:1345	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	3	59	theme	different	645:653	arg1	temperatures					655:666	different temperatures	645:666	different temperatures	645:666	Initially, mechanical properties of the alginate films prepared at different temperatures were evaluated to find the optimal temperature for preparing alginate solution.
36500449	2	60	theme	powder	300:305	arg1	effect					282:287	the effect	278:287	the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications	278:575	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	0	61	theme	Plasma-Activated	110:125	arg1	Water					127:131	Plasma-Activated Water	110:131	Plasma-Activated Water	110:131	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	11	62	theme	filler	1960:1965	arg1	potential					1939:1947	the potential	1935:1947	the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications	1935:2096	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	6	63	theme	plasma-activated	1068:1083	arg1	PAW					1092:1094	PAW	1092:1094	PAW	1092:1094	In addition, selected films were prepared in plasma-activated water (PAW).
36500449	6	63	theme	plasma-activated	1068:1083	arg1	water					1085:1089	plasma-activated water	1068:1089	plasma-activated water (PAW)	1068:1095	In addition, selected films were prepared in plasma-activated water (PAW).
36500449	10	64	theme	inhibitory	1711:1720	arg1	effect					1722:1727	A more pronounced inhibitory effect	1693:1727	A more pronounced inhibitory effect	1693:1727	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	4	65	theme	mechanical	819:828	arg1	properties					830:839	the mechanical properties	815:839	the mechanical properties of the alginate films	815:861	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	7	66	theme	significant	1271:1281	arg1	decrease					1283:1290	a significant decrease	1269:1290	a significant decrease	1269:1290	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	8	67	theme	improved	1386:1393	arg1	properties					1403:1412	improved barrier properties	1386:1412	improved barrier properties	1386:1412	The films prepared in PAW also showed improved barrier properties compared to those prepared in distilled water.
36500449	11	68	from	combination	1970:1980	arg1	potential					1939:1947	the potential	1935:1947	the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications	1935:2096	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	0	69	theme	Alginate	65:72	arg1	Films					74:78	Alginate Films	65:78	Alginate Films	65:78	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	5	70	theme	filler	987:992	arg1	addition					967:974	the addition	963:974	the addition of seaweed filler	963:992	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	9	71	theme	solution	1671:1678	arg1	concentration					1637:1649	the highest concentration	1625:1649	the highest concentration of the film-forming solution (83.3% v/v)	1625:1690	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	10	72	theme	cell	1895:1898	arg1	walls					1900:1904	the respective cell walls	1880:1904	the respective cell walls	1880:1904	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	2	73	from	effect	282:287	arg1	barrier					462:468	barrier	462:468	barrier	462:468	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	73	from	effect	282:287	arg1	mechanical					450:459	mechanical	450:459	mechanical	450:459	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	73	from	effect	282:287	arg1	properties					502:511	antimicrobial properties	488:511	antimicrobial properties	488:511	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	73	from	effect	282:287	arg1	antioxidant					471:481	antioxidant	471:481	antioxidant	471:481	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	4	74	theme	alginate	848:855	arg1	films					857:861	the alginate films	844:861	the alginate films	844:861	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	7	75	theme	tensile	1125:1131	arg1	strength					1133:1140	tensile strength	1125:1140	tensile strength	1125:1140	The mechanical properties (tensile strength, but not elongation at break) of the films prepared with PAW improved compared to the films prepared in distilled water, while a significant decrease was observed when incorporated with the seaweed filler.
36500449	5	76	theme	water	886:890	arg1	barrier					877:883	the barrier	873:883	the barrier (water vapor transmission rate)	873:915	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	5	76	theme	water	886:890	arg1	rate					911:914	water vapor transmission rate	886:914	water vapor transmission rate	886:914	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	11	77	from	filler	1960:1965	arg1	combination					1970:1980	combination	1970:1980	combination with PAW	1970:1989	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	0	78	theme	Powder	99:104	arg1	Effect					81:86	Effect	81:86	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.	0:132	Mechanical, Barrier, Antioxidant and Antimicrobial Properties of Alginate Films: Effect of Seaweed Powder and Plasma-Activated Water.
36500449	5	79	theme	transmission	898:909	arg1	barrier					877:883	the barrier	873:883	the barrier (water vapor transmission rate)	873:915	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	5	79	theme	transmission	898:909	arg1	rate					911:914	water vapor transmission rate	886:914	water vapor transmission rate	886:914	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	2	80	from	sizes	420:424	arg1	bio-filler					312:321	a bio-filler	310:321	a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm)	310:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	80	from	sizes	420:424	arg1	films					341:345	alginate-based films	326:345	alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm)	326:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	10	81	located	observed	1733:1740	arg1	aureus					1766:1771	the Gram-positive S. aureus	1745:1771	the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls	1745:1904	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	10	81	located	observed	1733:1740	arg2	effect					1722:1727	A more pronounced inhibitory effect	1693:1727	A more pronounced inhibitory effect	1693:1727	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	11	82	theme	packaging	2075:2083	arg1	applications					2085:2096	potential food packaging applications	2060:2096	potential food packaging applications	2060:2096	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	5	83	theme	barrier	877:883	arg1	properties					933:942	the barrier (water vapor transmission rate) and antioxidant properties	873:942	the barrier (water vapor transmission rate) and antioxidant properties	873:942	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	11	84	theme	potential	2060:2068	arg1	applications					2085:2096	potential food packaging applications	2060:2096	potential food packaging applications	2060:2096	This study has demonstrated the potential of seaweed filler in combination with PAW towards enhanced functionality and bioactivity of alginate films for potential food packaging applications.
36500449	9	85	theme	film-forming	1512:1523	arg1	solution					1525:1532	the alginate-seaweed film-forming solution	1491:1532	the alginate-seaweed film-forming solution	1491:1532	The antimicrobial activity of the alginate-seaweed film-forming solution was in general more pronounced when prepared with PAW and stored at 10 °C, particularly at the highest concentration of the film-forming solution (83.3% v/v).
36500449	2	86	theme	particle	411:418	arg1	μm					439:440	100 and 200 μm	427:440	100 and 200 μm	427:440	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	86	theme	particle	411:418	arg1	sizes					420:424	particle sizes	411:424	particle sizes (100 and 200 μm)	411:441	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	8	87	theme	distilled	1444:1452	arg1	water					1454:1458	distilled water	1444:1458	distilled water	1444:1458	The films prepared in PAW also showed improved barrier properties compared to those prepared in distilled water.
36500449	4	88	theme	seaweed	764:770	arg1	powder					772:777	seaweed powder	764:777	seaweed powder	764:777	The addition of seaweed powder did not have any positive effect on the mechanical properties of the alginate films.
36500449	10	89	theme	walls	1900:1904	arg1	composition					1851:1861	different composition	1841:1861	different composition	1841:1861	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	10	89	theme	walls	1900:1904	arg1	structure					1867:1875	structure	1867:1875	structure	1867:1875	A more pronounced inhibitory effect was observed on the Gram-positive S. aureus than on the Gram-negative E. coli, which has been attributed to the different composition and structure of the respective cell walls.
36500449	2	90	dep	concentrations	360:373	arg1	%					390:390	10, 30, and 50%	376:390	10, 30, and 50%	376:390	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	2	90	dep	concentrations	360:373	arg1	alginate					397:404	w/w alginate	393:404	w/w alginate	393:404	In this study, we investigated the effect of seaweed powder as a bio-filler in alginate-based films at different concentrations (10, 30, and 50%, w/w alginate) and particle sizes (100 and 200 μm) on the mechanical, barrier, antioxidant, and antimicrobial properties of alginate which are essential for food packaging applications.
36500449	5	91	theme	antioxidant	921:931	arg1	properties					933:942	the barrier (water vapor transmission rate) and antioxidant properties	873:942	the barrier (water vapor transmission rate) and antioxidant properties	873:942	However, the barrier (water vapor transmission rate) and antioxidant properties were improved with the addition of seaweed filler regardless of concentration.
36500449	6	92	theme	selected	1036:1043	arg1	films					1045:1049	selected films	1036:1049	selected films	1036:1049	In addition, selected films were prepared in plasma-activated water (PAW).
36500449	3	93	theme	films	627:631	arg1	properties					600:609	mechanical properties	589:609	mechanical properties of the alginate films prepared at different temperatures	589:666	Initially, mechanical properties of the alginate films prepared at different temperatures were evaluated to find the optimal temperature for preparing alginate solution.
36500449	1	94	theme	films	240:244	arg1	properties					215:224	the properties	211:224	the properties of biopolymer films	211:244	The incorporation of natural fillers such as seaweed may potentially enhance the properties of biopolymer films.
36499146	1	0	theme	chitosan	168:175	arg1	scaffolds					177:185	chitosan scaffolds	168:185	chitosan scaffolds	168:185	It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body.
36499146	0	1	theme	Biological	66:75	arg1	Properties					77:86	the Physicochemical, Mechanical, and Biological Properties	29:86	the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	29:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	5	2	theme	cell	1086:1089	arg1	proliferation					1091:1103	better cell proliferation	1079:1103	better cell proliferation	1079:1103	The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology.
36499146	0	3	theme	Ammonium-Hydroxide-Crosslinked	91:120	arg1	Scaffolds					131:139	Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	91:139	Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	91:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	0	4	from	Effects	0:6	arg1	Properties					77:86	the Physicochemical, Mechanical, and Biological Properties	29:86	the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	29:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	6	5	theme	high	1167:1170	arg1	pH					1172:1173	the chitosan scaffolds' high pH	1143:1173	the chitosan scaffolds' high pH (due to residual ammonium hydroxide)	1143:1210	It was concluded that the chitosan scaffolds' high pH (due to residual ammonium hydroxide) decreases DPSCs' cell viability.
36499146	2	6	theme	biological	365:374	arg1	properties					376:385	the physicochemical, mechanical, and biological properties	328:385	the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide	328:443	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	3	7	theme	mechanical	907:916	arg1	testing					918:924	mechanical testing	907:924	mechanical testing	907:924	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	1	8	theme	human	286:290	arg1	body					292:295	the human body	282:295	the human body	282:295	It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body.
36499146	0	9	theme	Scaffolds	131:139	arg1	Properties					77:86	the Physicochemical, Mechanical, and Biological Properties	29:86	the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	29:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	0	10	theme	Chitosan	122:129	arg1	Scaffolds					131:139	Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	91:139	Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	91:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	3	11	theme	scanning	736:743	arg1	SEM					766:768	SEM	766:768	SEM	766:768	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	3	11	theme	scanning	736:743	arg1	microscopy					754:763	scanning electron microscopy	736:763	scanning electron microscopy (SEM)	736:769	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	0	12	theme	Physicochemical	33:47	arg1	Properties					77:86	the Physicochemical, Mechanical, and Biological Properties	29:86	the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	29:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	4	13	theme	dental-pulp	966:976	arg1	cells					983:987	dental-pulp stem cells	966:987	dental-pulp stem cells' (DPSCs') biocompatibility	966:1014	In vitro cytotoxicity was assessed via dental-pulp stem cells' (DPSCs') biocompatibility.
36499146	4	13	theme	dental-pulp	966:976	arg1	DPSCs					991:995	DPSCs'	991:996	DPSCs'	991:996	In vitro cytotoxicity was assessed via dental-pulp stem cells' (DPSCs') biocompatibility.
36499146	4	14	dep	In	927:928	arg1	vitro					930:934	vitro	930:934	vitro	930:934	In vitro cytotoxicity was assessed via dental-pulp stem cells' (DPSCs') biocompatibility.
36499146	3	15	theme	electron	745:752	arg1	SEM					766:768	SEM	766:768	SEM	766:768	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	3	15	theme	electron	745:752	arg1	microscopy					754:763	scanning electron microscopy	736:763	scanning electron microscopy (SEM)	736:769	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	3	16	theme	sodium	586:591	arg1	solution					603:610	sodium hydroxide solution	586:610	sodium hydroxide solution	586:610	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	2	17	theme	scaffolds	399:407	arg1	properties					376:385	the physicochemical, mechanical, and biological properties	328:385	the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide	328:443	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	6	18	theme	ammonium	1192:1199	arg1	hydroxide					1201:1209	residual ammonium hydroxide	1183:1209	residual ammonium hydroxide	1183:1209	It was concluded that the chitosan scaffolds' high pH (due to residual ammonium hydroxide) decreases DPSCs' cell viability.
36499146	1	19	theme	body	292:295	arg1	tissues					271:277	different tissues	261:277	different tissues of the human body	261:295	It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body.
36499146	3	20	theme	hydroxide	593:601	arg1	solution					603:610	sodium hydroxide solution	586:610	sodium hydroxide solution	586:610	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	2	21	theme	chitosan	390:397	arg1	scaffolds					399:407	chitosan scaffolds	390:407	chitosan scaffolds crosslinked with ammonium hydroxide	390:443	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	2	22	theme	pH	461:462	arg1	values					464:469	different pH values	451:469	different pH values	451:469	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	3	23	theme	elemental	772:780	arg1	EDX					795:797	EDX	795:797	EDX	795:797	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	3	23	theme	elemental	772:780	arg1	composition					782:792	elemental composition	772:792	elemental composition (EDX)	772:798	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	4	24	theme	In	927:928	arg1	cytotoxicity					936:947	In vitro cytotoxicity	927:947	In vitro cytotoxicity	927:947	In vitro cytotoxicity was assessed via dental-pulp stem cells' (DPSCs') biocompatibility.
36499146	1	25	theme	physicochemical	201:215	arg1	properties					217:226	their physicochemical properties	195:226	their physicochemical properties	195:226	It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body.
36499146	0	26	theme	Neutralization	11:24	arg1	Effects					0:6	Effects	0:6	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	0:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	3	27	dep	characterization	710:725	arg1	pH.					690:692	pH.	690:692	pH.	690:692	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	6	28	theme	chitosan	1147:1154	arg1	pH					1172:1173	the chitosan scaffolds' high pH	1143:1173	the chitosan scaffolds' high pH (due to residual ammonium hydroxide)	1143:1210	It was concluded that the chitosan scaffolds' high pH (due to residual ammonium hydroxide) decreases DPSCs' cell viability.
36499146	2	29	theme	cell	493:496	arg1	behavior					498:505	cell behavior	493:505	cell behavior	493:505	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	5	30	theme	better	1079:1084	arg1	proliferation					1091:1103	better cell proliferation	1079:1103	better cell proliferation	1079:1103	The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology.
36499146	6	31	theme	cell	1229:1232	arg1	viability					1234:1242	DPSCs' cell viability	1222:1242	DPSCs' cell viability	1222:1242	It was concluded that the chitosan scaffolds' high pH (due to residual ammonium hydroxide) decreases DPSCs' cell viability.
36499146	3	32	dep	infrared	819:826	arg1	FTIR					829:832	FTIR	829:832	FTIR	829:832	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	2	33	theme	ammonium	426:433	arg1	hydroxide					435:443	ammonium hydroxide	426:443	ammonium hydroxide	426:443	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	0	34	theme	Mechanical	50:59	arg1	Properties					77:86	the Physicochemical, Mechanical, and Biological Properties	29:86	the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	29:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	2	35	theme	biomaterial	534:544	arg1	pH					524:525	the pH	520:525	the pH of the biomaterial	520:544	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	3	36	theme	neutral	658:664	arg1	pH					666:667	a neutral pH	656:667	a neutral pH	656:667	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	3	37	theme	Physicochemical	694:708	arg1	characterization					710:725	Physicochemical characterization	694:725	Physicochemical characterization	694:725	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	3	38	theme	Fourier-transform	801:817	arg1	spectroscopy					835:846	Fourier-transform infrared (FTIR) spectroscopy	801:846	Fourier-transform infrared (FTIR) spectroscopy	801:846	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	2	39	theme	mechanical	349:358	arg1	properties					376:385	the physicochemical, mechanical, and biological properties	328:385	the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide	328:443	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	6	40	theme	residual	1183:1190	arg1	hydroxide					1201:1209	residual ammonium hydroxide	1183:1209	residual ammonium hydroxide	1183:1209	It was concluded that the chitosan scaffolds' high pH (due to residual ammonium hydroxide) decreases DPSCs' cell viability.
36499146	3	41	theme	thermogravimetric	869:885	arg1	TGA					897:899	TGA	897:899	TGA	897:899	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	3	41	theme	thermogravimetric	869:885	arg1	analysis					887:894	thermogravimetric analysis	869:894	thermogravimetric analysis (TGA)	869:900	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	1	42	theme	cell	239:242	arg1	proliferation					244:256	cell proliferation	239:256	cell proliferation	239:256	It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body.
36499146	5	43	theme	neutralized	1047:1057	arg1	scaffolds					1059:1067	the neutralized scaffolds	1043:1067	the neutralized scaffolds	1043:1067	The results revealed that the neutralized scaffolds exhibited better cell proliferation and morphology.
36499146	2	44	theme	physicochemical	332:346	arg1	properties					376:385	the physicochemical, mechanical, and biological properties	328:385	the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide	328:443	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	3	45	theme	Raman	849:853	arg1	spectroscopy					855:866	Raman spectroscopy	849:866	Raman spectroscopy	849:866	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	4	46	theme	cells	983:987	arg1	biocompatibility					999:1014	dental-pulp stem cells' (DPSCs') biocompatibility	966:1014	dental-pulp stem cells' (DPSCs') biocompatibility	966:1014	In vitro cytotoxicity was assessed via dental-pulp stem cells' (DPSCs') biocompatibility.
36499146	3	47	theme	distilled	625:633	arg1	water					635:639	distilled water	625:639	distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing	625:924	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	4	48	theme	stem	978:981	arg1	cells					983:987	dental-pulp stem cells	966:987	dental-pulp stem cells' (DPSCs') biocompatibility	966:1014	In vitro cytotoxicity was assessed via dental-pulp stem cells' (DPSCs') biocompatibility.
36499146	4	48	theme	stem	978:981	arg1	DPSCs					991:995	DPSCs'	991:996	DPSCs'	991:996	In vitro cytotoxicity was assessed via dental-pulp stem cells' (DPSCs') biocompatibility.
36499146	2	49	theme	different	451:459	arg1	values					464:469	different pH values	451:469	different pH values	451:469	This study aimed to determine the physicochemical, mechanical, and biological properties of chitosan scaffolds crosslinked with ammonium hydroxide, with different pH values, to better understand cell behavior depending on the pH of the biomaterial.
36499146	0	50	link	Ammonium-Hydroxide-Crosslinked	91:120	arg1	Scaffolds					131:139	Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	91:139	Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds	91:139	Effects of Neutralization on the Physicochemical, Mechanical, and Biological Properties of Ammonium-Hydroxide-Crosslinked Chitosan Scaffolds.
36499146	3	51	theme	infrared	819:826	arg1	spectroscopy					835:846	Fourier-transform infrared (FTIR) spectroscopy	801:846	Fourier-transform infrared (FTIR) spectroscopy	801:846	Scaffolds were either neutralized with sodium hydroxide solution, washed with distilled water until reaching a neutral pH, or kept at alkaline pH. Physicochemical characterization included scanning electron microscopy (SEM), elemental composition (EDX), Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, thermogravimetric analysis (TGA), and mechanical testing.
36499146	1	52	theme	different	261:269	arg1	tissues					271:277	different tissues	261:277	different tissues of the human body	261:295	It has been reported that chitosan scaffolds, due to their physicochemical properties, stimulate cell proliferation in different tissues of the human body.
36541457	6	0	theme	intracellular	906:918	arg1	concentration					920:932	Their intracellular concentration	900:932	Their intracellular concentration	900:932	Their intracellular concentration, localization and effect on the viability of MDA-MB-231 cells were assessed.
36541457	1	1	theme	cancer	301:306	arg1	signaling					233:241	immune checkpoint signaling	215:241	immune checkpoint signaling	215:241	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	1	theme	cancer	301:306	arg1	reprogramming					180:192	Metabolic reprogramming	170:192	Metabolic reprogramming 'Warburg effect'	170:209	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	1	theme	cancer	301:306	arg1	hallmarks					265:273	immunosuppressive hallmarks	247:273	immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy	247:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	2	dep	reprogramming	180:192	arg1	effect					203:208	Warburg effect	195:208	Warburg effect	195:208	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	10	3	theme	checkpoint	1648:1657	arg1	signaling					1659:1667	immune checkpoint signaling	1641:1667	immune checkpoint signaling of TNBC cells	1641:1681	Chitosan, lecithin and the NPs-formulated forms have been shown to influence the 'Warburg effect' and immune checkpoint signaling of TNBC cells differently.
36541457	2	4	used	used	481:484	arg2	microenvironment					452:467	the tumor microenvironment	442:467	the tumor microenvironment that can be used alongside conventional immunotherapeutics	442:526	Biomaterials arise as novel tools for immunomodulation of the tumor microenvironment that can be used alongside conventional immunotherapeutics.
36541457	3	5	theme	biomaterials	577:588	arg1	Chitosan					529:536	Chitosan	529:536	Chitosan	529:536	Chitosan and lecithin are examples of versatile biomaterials with interesting immunomodulatory properties.
36541457	3	5	theme	biomaterials	577:588	arg1	examples					555:562	examples	555:562	examples of versatile biomaterials with interesting immunomodulatory properties	555:633	Chitosan and lecithin are examples of versatile biomaterials with interesting immunomodulatory properties.
36541457	3	5	theme	biomaterials	577:588	arg1	lecithin					542:549	lecithin	542:549	lecithin	542:549	Chitosan and lecithin are examples of versatile biomaterials with interesting immunomodulatory properties.
36541457	4	6	from	mediators	748:756	arg1	investigation					663:675	investigation	663:675	investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	663:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	5	7	theme	lecithin-chitosan	845:861	arg1	NPs					863:865	Hybrid lecithin-chitosan NPs	838:865	Hybrid lecithin-chitosan NPs	838:865	Hybrid lecithin-chitosan NPs were prepared and characterized.
36541457	6	8	theme	cells	990:994	arg1	viability					966:974	the viability	962:974	the viability of MDA-MB-231 cells	962:994	Their intracellular concentration, localization and effect on the viability of MDA-MB-231 cells were assessed.
36541457	9	9	theme	polymerase	1501:1510	arg1	q-RT-PCR					1528:1535	q-RT-PCR	1528:1535	q-RT-PCR	1528:1535	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	9	theme	polymerase	1501:1510	arg1	reaction					1518:1525	quantitative reverse transcription polymerase chain reaction	1466:1525	quantitative reverse transcription polymerase chain reaction (q-RT-PCR)	1466:1536	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	6	10	from	effect	952:957	arg1	viability					966:974	the viability	962:974	the viability of MDA-MB-231 cells	962:994	Their intracellular concentration, localization and effect on the viability of MDA-MB-231 cells were assessed.
36541457	9	11	theme	kinase	1341:1346	arg1	B					1348:1348	protein kinase B	1333:1348	protein kinase B (PKB or Akt)	1333:1361	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	12	theme	phosphatidylinositol-3-kinase	1295:1323	arg1	expression					1241:1250	Gene expression	1236:1250	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1	1236:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	4	13	theme	nanoparticles	718:730	arg1	role					684:687	the role	680:687	the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	680:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	1	14	theme	checkpoint	222:231	arg1	reprogramming					180:192	Metabolic reprogramming	170:192	Metabolic reprogramming 'Warburg effect'	170:209	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	14	theme	checkpoint	222:231	arg1	signaling					233:241	immune checkpoint signaling	215:241	immune checkpoint signaling	215:241	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	14	theme	checkpoint	222:231	arg1	hallmarks					265:273	immunosuppressive hallmarks	247:273	immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy	247:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	6	15	from	concentration	920:932	arg1	viability					966:974	the viability	962:974	the viability of MDA-MB-231 cells	962:994	Their intracellular concentration, localization and effect on the viability of MDA-MB-231 cells were assessed.
36541457	0	16	theme	breast	155:160	arg1	cancer					162:167	triple-negative breast cancer	139:167	triple-negative breast cancer	139:167	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	7	17	theme	lactate	1123:1129	arg1	consumption					1069:1079	glucose consumption	1061:1079	glucose consumption	1061:1079	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	7	17	theme	lactate	1123:1129	arg1	production					1131:1140	lactate production	1123:1140	lactate production	1123:1140	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	6	18	from	localization	935:946	arg1	viability					966:974	the viability	962:974	the viability of MDA-MB-231 cells	962:994	Their intracellular concentration, localization and effect on the viability of MDA-MB-231 cells were assessed.
36541457	7	19	theme	extracellular	1146:1158	arg1	acidification					1160:1172	extracellular acidification	1146:1172	extracellular acidification	1146:1172	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	7	19	theme	extracellular	1146:1158	arg1	consumption					1069:1079	glucose consumption	1061:1079	glucose consumption	1061:1079	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	9	20	theme	mammalian	1364:1372	arg1	target					1374:1379	mammalian target	1364:1379	mammalian target of rapamycin (mTOR)	1364:1399	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	0	21	from	signaling	98:106	arg1	cancer					162:167	triple-negative breast cancer	139:167	triple-negative breast cancer	139:167	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	11	22	theme	immunosuppressive	1823:1839	arg1	characteristics					1841:1855	the immunosuppressive characteristics	1819:1855	the immunosuppressive characteristics of TNBC cells	1819:1869	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	1	23	theme	immunotherapy	369:381	arg1	applicability					352:364	the limited clinical applicability	331:364	the limited clinical applicability of immunotherapy	331:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	7	24	theme	triphosphate	1092:1103	arg1	consumption					1069:1079	glucose consumption	1061:1079	glucose consumption	1061:1079	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	7	24	theme	triphosphate	1092:1103	arg1	generation					1111:1120	adenosine triphosphate (ATP) generation	1082:1120	adenosine triphosphate (ATP) generation	1082:1120	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	1	25	theme	Metabolic	170:178	arg1	signaling					233:241	immune checkpoint signaling	215:241	immune checkpoint signaling	215:241	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	25	theme	Metabolic	170:178	arg1	reprogramming					180:192	Metabolic reprogramming	170:192	Metabolic reprogramming 'Warburg effect'	170:209	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	25	theme	Metabolic	170:178	arg1	hallmarks					265:273	immunosuppressive hallmarks	247:273	immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy	247:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	4	26	theme	death	777:781	arg1	ligand					783:788	death ligand 1	777:790	programmed death ligand 1 (PD-L1) expression	766:809	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	4	26	theme	death	777:781	arg1	PD-L1					793:797	PD-L1	793:797	PD-L1	793:797	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	0	27	theme	immune	80:85	arg1	signaling					98:106	immune checkpoint signaling	80:106	immune checkpoint signaling	80:106	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	11	28	theme	cells	1865:1869	arg1	characteristics					1841:1855	the immunosuppressive characteristics	1819:1855	the immunosuppressive characteristics of TNBC cells	1819:1869	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	7	29	theme	glucose	1061:1067	arg1	acidification					1160:1172	extracellular acidification	1146:1172	extracellular acidification	1146:1172	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	7	29	theme	glucose	1061:1067	arg1	consumption					1069:1079	glucose consumption	1061:1079	glucose consumption	1061:1079	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	7	29	theme	glucose	1061:1067	arg1	generation					1111:1120	adenosine triphosphate (ATP) generation	1082:1120	adenosine triphosphate (ATP) generation	1082:1120	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	7	29	theme	glucose	1061:1067	arg1	production					1131:1140	lactate production	1123:1140	lactate production	1123:1140	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	2	30	theme	tumor	446:450	arg1	microenvironment					452:467	the tumor microenvironment	442:467	the tumor microenvironment that can be used alongside conventional immunotherapeutics	442:526	Biomaterials arise as novel tools for immunomodulation of the tumor microenvironment that can be used alongside conventional immunotherapeutics.
36541457	0	31	theme	metabolic	112:120	arg1	reprogramming					122:134	metabolic reprogramming	112:134	metabolic reprogramming	112:134	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	9	32	theme	hypoxia-inducible	1402:1418	arg1	HIF-1α					1430:1435	HIF-1α	1430:1435	HIF-1α	1430:1435	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	32	theme	hypoxia-inducible	1402:1418	arg1	factor					1420:1425	hypoxia-inducible factor 1α	1402:1428	hypoxia-inducible factor 1α(HIF-1α)	1402:1436	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	8	33	theme	Nitric	1175:1180	arg1	production					1188:1197	Nitric oxide production	1175:1197	Nitric oxide production	1175:1197	Nitric oxide production was quantified using Greiss reagent.
36541457	4	34	theme	expression	800:809	arg1	mediators					748:756	common mediators	741:756	common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	741:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	12	35	theme	expression	2044:2053	arg1	reversal					2059:2066	reversal	2059:2066	reversal of the Warburg effect	2059:2088	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	12	35	theme	expression	2044:2053	arg1	reduction					2025:2033	reduction	2025:2033	reduction of PD-L1 expression	2025:2053	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	4	36	theme	glycolytic	815:824	arg1	metabolism					826:835	glycolytic metabolism	815:835	glycolytic metabolism	815:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	9	37	theme	Gene	1236:1239	arg1	expression					1241:1250	Gene expression	1236:1250	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1	1236:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	12	38	theme	immune-active	1990:2002	arg1	microenvironment					2004:2019	an immune-active microenvironment	1987:2019	an immune-active microenvironment	1987:2019	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	1	39	theme	limited	335:341	arg1	applicability					352:364	the limited clinical applicability	331:364	the limited clinical applicability of immunotherapy	331:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	8	40	theme	Greiss	1220:1225	arg1	reagent					1227:1233	Greiss reagent	1220:1233	Greiss reagent	1220:1233	Nitric oxide production was quantified using Greiss reagent.
36541457	9	41	theme	nitric	1265:1270	arg1	iNOS					1288:1291	iNOS	1288:1291	iNOS	1288:1291	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	41	theme	nitric	1265:1270	arg1	synthase					1278:1285	inducible nitric oxide synthase	1255:1285	inducible nitric oxide synthase (iNOS)	1255:1292	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	0	42	theme	intertwined	43:53	arg1	pathways					55:62	intertwined pathways	43:62	intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer	43:167	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	9	43	theme	synthase	1278:1285	arg1	expression					1241:1250	Gene expression	1236:1250	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1	1236:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	1	44	theme	immunosuppressive	247:263	arg1	signaling					233:241	immune checkpoint signaling	215:241	immune checkpoint signaling	215:241	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	44	theme	immunosuppressive	247:263	arg1	reprogramming					180:192	Metabolic reprogramming	170:192	Metabolic reprogramming 'Warburg effect'	170:209	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	44	theme	immunosuppressive	247:263	arg1	hallmarks					265:273	immunosuppressive hallmarks	247:273	immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy	247:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	12	45	theme	lecithin-chitosan	1900:1916	arg1	NPs					1918:1920	Carefully engineered hybrid lecithin-chitosan NPs	1872:1920	Carefully engineered hybrid lecithin-chitosan NPs	1872:1920	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	4	46	theme	role	684:687	arg1	investigation					663:675	investigation	663:675	investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	663:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	3	47	theme	interesting	595:605	arg1	properties					624:633	interesting immunomodulatory properties	595:633	interesting immunomodulatory properties	595:633	Chitosan and lecithin are examples of versatile biomaterials with interesting immunomodulatory properties.
36541457	9	48	theme	reverse	1479:1485	arg1	q-RT-PCR					1528:1535	q-RT-PCR	1528:1535	q-RT-PCR	1528:1535	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	48	theme	reverse	1479:1485	arg1	reaction					1518:1525	quantitative reverse transcription polymerase chain reaction	1466:1525	quantitative reverse transcription polymerase chain reaction (q-RT-PCR)	1466:1536	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	49	theme	PD-L1	1442:1446	arg1	expression					1241:1250	Gene expression	1236:1250	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1	1236:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	10	50	theme	Warburg	1621:1627	arg1	effect					1629:1634	the 'Warburg effect'	1616:1635	the 'Warburg effect'	1616:1635	Chitosan, lecithin and the NPs-formulated forms have been shown to influence the 'Warburg effect' and immune checkpoint signaling of TNBC cells differently.
36541457	5	51	theme	Hybrid	838:843	arg1	NPs					863:865	Hybrid lecithin-chitosan NPs	838:865	Hybrid lecithin-chitosan NPs	838:865	Hybrid lecithin-chitosan NPs were prepared and characterized.
36541457	3	52	with	biomaterials	577:588	arg1	properties					624:633	interesting immunomodulatory properties	595:633	interesting immunomodulatory properties	595:633	Chitosan and lecithin are examples of versatile biomaterials with interesting immunomodulatory properties.
36541457	11	53	theme	subcellular	1749:1759	arg1	localization					1761:1772	their subcellular localization	1743:1772	their subcellular localization	1743:1772	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	2	54	theme	novel	406:410	arg1	tools					412:416	novel tools	406:416	novel tools for immunomodulation of the tumor microenvironment that can be used alongside conventional immunotherapeutics	406:526	Biomaterials arise as novel tools for immunomodulation of the tumor microenvironment that can be used alongside conventional immunotherapeutics.
36541457	1	55	theme	breast	294:299	arg1	cancer					301:306	triple-negative breast cancer	278:306	triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy	278:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	55	theme	breast	294:299	arg1	TNBC					309:312	TNBC	309:312	TNBC	309:312	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	11	56	theme	hybrid	1719:1724	arg1	systems					1726:1732	the hybrid systems	1715:1732	the hybrid systems	1715:1732	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	12	57	theme	engineered	1882:1891	arg1	NPs					1918:1920	Carefully engineered hybrid lecithin-chitosan NPs	1872:1920	Carefully engineered hybrid lecithin-chitosan NPs	1872:1920	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	10	58	theme	immune	1641:1646	arg1	signaling					1659:1667	immune checkpoint signaling	1641:1667	immune checkpoint signaling of TNBC cells	1641:1681	Chitosan, lecithin and the NPs-formulated forms have been shown to influence the 'Warburg effect' and immune checkpoint signaling of TNBC cells differently.
36541457	1	59	theme	Warburg	195:201	arg1	effect					203:208	Warburg effect	195:208	Warburg effect	195:208	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	6	60	theme	MDA-MB-231	979:988	arg1	cells					990:994	MDA-MB-231 cells	979:994	MDA-MB-231 cells	979:994	Their intracellular concentration, localization and effect on the viability of MDA-MB-231 cells were assessed.
36541457	10	61	theme	cells	1677:1681	arg1	effect					1629:1634	the 'Warburg effect'	1616:1635	the 'Warburg effect'	1616:1635	Chitosan, lecithin and the NPs-formulated forms have been shown to influence the 'Warburg effect' and immune checkpoint signaling of TNBC cells differently.
36541457	10	61	theme	cells	1677:1681	arg1	signaling					1659:1667	immune checkpoint signaling	1641:1667	immune checkpoint signaling of TNBC cells	1641:1681	Chitosan, lecithin and the NPs-formulated forms have been shown to influence the 'Warburg effect' and immune checkpoint signaling of TNBC cells differently.
36541457	4	62	from	role	684:687	arg1	mediators					748:756	common mediators	741:756	common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	741:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	9	63	theme	protein	1333:1339	arg1	B					1348:1348	protein kinase B	1333:1348	protein kinase B (PKB or Akt)	1333:1361	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	4	64	theme	common	741:746	arg1	mediators					748:756	common mediators	741:756	common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	741:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	10	65	theme	TNBC	1672:1675	arg1	cells					1677:1681	TNBC cells	1672:1681	TNBC cells	1672:1681	Chitosan, lecithin and the NPs-formulated forms have been shown to influence the 'Warburg effect' and immune checkpoint signaling of TNBC cells differently.
36541457	0	66	theme	triple-negative	139:153	arg1	cancer					162:167	triple-negative breast cancer	139:167	triple-negative breast cancer	139:167	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	9	67	theme	B	1348:1348	arg1	expression					1241:1250	Gene expression	1236:1250	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1	1236:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	1	68	theme	immune	215:220	arg1	reprogramming					180:192	Metabolic reprogramming	170:192	Metabolic reprogramming 'Warburg effect'	170:209	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	68	theme	immune	215:220	arg1	signaling					233:241	immune checkpoint signaling	215:241	immune checkpoint signaling	215:241	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	68	theme	immune	215:220	arg1	hallmarks					265:273	immunosuppressive hallmarks	247:273	immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy	247:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	4	69	theme	hybrid	711:716	arg1	NPs					733:735	NPs	733:735	NPs	733:735	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	4	69	theme	hybrid	711:716	arg1	nanoparticles					718:730	carefully designed hybrid nanoparticles	692:730	carefully designed hybrid nanoparticles (NPs)	692:736	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	12	70	theme	effect	2083:2088	arg1	reversal					2059:2066	reversal	2059:2066	reversal of the Warburg effect	2059:2088	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	12	70	theme	effect	2083:2088	arg1	reduction					2025:2033	reduction	2025:2033	reduction of PD-L1 expression	2025:2053	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	0	71	from	reprogramming	122:134	arg1	cancer					162:167	triple-negative breast cancer	139:167	triple-negative breast cancer	139:167	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	4	72	theme	ligand	783:788	arg1	expression					800:809	programmed death ligand 1 (PD-L1) expression	766:809	programmed death ligand 1 (PD-L1) expression	766:809	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	11	73	theme	TNBC	1860:1863	arg1	cells					1865:1869	TNBC cells	1860:1869	TNBC cells	1860:1869	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	9	74	theme	target	1374:1379	arg1	expression					1241:1250	Gene expression	1236:1250	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1	1236:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	11	75	from	impact	1809:1814	arg1	characteristics					1841:1855	the immunosuppressive characteristics	1819:1855	the immunosuppressive characteristics of TNBC cells	1819:1869	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	0	76	theme	checkpoint	87:96	arg1	signaling					98:106	immune checkpoint signaling	80:106	immune checkpoint signaling	80:106	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	9	77	theme	rapamycin	1384:1392	arg1	PI3K					1326:1329	PI3K	1326:1329	PI3K	1326:1329	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	factor					1420:1425	hypoxia-inducible factor 1α	1402:1428	hypoxia-inducible factor 1α(HIF-1α)	1402:1436	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	PD-L1					1442:1446	PD-L1	1442:1446	PD-L1	1442:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	iNOS					1288:1291	iNOS	1288:1291	iNOS	1288:1291	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	B					1348:1348	protein kinase B	1333:1348	protein kinase B (PKB or Akt)	1333:1361	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	HIF-1α					1430:1435	HIF-1α	1430:1435	HIF-1α	1430:1435	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	phosphatidylinositol-3-kinase					1295:1323	phosphatidylinositol-3-kinase	1295:1323	phosphatidylinositol-3-kinase (PI3K)	1295:1330	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	synthase					1278:1285	inducible nitric oxide synthase	1255:1285	inducible nitric oxide synthase (iNOS)	1255:1292	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	77	theme	rapamycin	1384:1392	arg1	target					1374:1379	mammalian target	1364:1379	mammalian target of rapamycin (mTOR)	1364:1399	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	78	dep	B	1348:1348	arg1	Akt					1358:1360	Akt	1358:1360	Akt	1358:1360	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	78	dep	B	1348:1348	arg1	PKB					1351:1353	PKB	1351:1353	PKB	1351:1353	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	4	79	theme	programmed	766:775	arg1	expression					800:809	programmed death ligand 1 (PD-L1) expression	766:809	programmed death ligand 1 (PD-L1) expression	766:809	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	12	80	theme	Warburg	2075:2081	arg1	effect					2083:2088	the Warburg effect	2071:2088	the Warburg effect	2071:2088	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	7	81	theme	adenosine	1082:1090	arg1	ATP					1106:1108	ATP	1106:1108	ATP	1106:1108	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	7	81	theme	adenosine	1082:1090	arg1	triphosphate					1092:1103	adenosine triphosphate	1082:1103	adenosine triphosphate (ATP) generation	1082:1120	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	4	82	theme	metabolism	826:835	arg1	mediators					748:756	common mediators	741:756	common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	741:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	4	83	from	investigation	663:675	arg1	mediators					748:756	common mediators	741:756	common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism	741:835	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	8	84	theme	oxide	1182:1186	arg1	production					1188:1197	Nitric oxide production	1175:1197	Nitric oxide production	1175:1197	Nitric oxide production was quantified using Greiss reagent.
36541457	9	85	theme	chain	1512:1516	arg1	q-RT-PCR					1528:1535	q-RT-PCR	1528:1535	q-RT-PCR	1528:1535	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	85	theme	chain	1512:1516	arg1	reaction					1518:1525	quantitative reverse transcription polymerase chain reaction	1466:1525	quantitative reverse transcription polymerase chain reaction (q-RT-PCR)	1466:1536	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	86	theme	factor	1420:1425	arg1	expression					1241:1250	Gene expression	1236:1250	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1	1236:1446	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	2	87	theme	conventional	496:507	arg1	immunotherapeutics					509:526	conventional immunotherapeutics	496:526	conventional immunotherapeutics	496:526	Biomaterials arise as novel tools for immunomodulation of the tumor microenvironment that can be used alongside conventional immunotherapeutics.
36541457	12	88	theme	PD-L1	2038:2042	arg1	expression					2044:2053	PD-L1 expression	2038:2053	PD-L1 expression	2038:2053	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	11	89	theme	positive	1788:1795	arg1	impact					1809:1814	the positive or negative impact	1784:1814	the positive or negative impact on the immunosuppressive characteristics of TNBC cells	1784:1869	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	10	90	theme	NPs-formulated	1566:1579	arg1	forms					1581:1585	the NPs-formulated forms	1562:1585	the NPs-formulated forms	1562:1585	Chitosan, lecithin and the NPs-formulated forms have been shown to influence the 'Warburg effect' and immune checkpoint signaling of TNBC cells differently.
36541457	9	91	theme	inducible	1255:1263	arg1	iNOS					1288:1291	iNOS	1288:1291	iNOS	1288:1291	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	91	theme	inducible	1255:1263	arg1	synthase					1278:1285	inducible nitric oxide synthase	1255:1285	inducible nitric oxide synthase (iNOS)	1255:1292	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	2	92	theme	microenvironment	452:467	arg1	immunomodulation					422:437	immunomodulation	422:437	immunomodulation of the tumor microenvironment that can be used alongside conventional immunotherapeutics	422:526	Biomaterials arise as novel tools for immunomodulation of the tumor microenvironment that can be used alongside conventional immunotherapeutics.
36541457	0	93	theme	pathways	55:62	arg1	array					34:38	tune-up an array	23:38	tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer	23:167	Drugless nanoparticles tune-up an array of intertwined pathways contributing to immune checkpoint signaling and metabolic reprogramming in triple-negative breast cancer.
36541457	1	94	theme	clinical	343:350	arg1	applicability					352:364	the limited clinical applicability	331:364	the limited clinical applicability of immunotherapy	331:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	12	95	theme	TNBC	1979:1982	arg1	microenvironment					1959:1974	the immune-suppressive microenvironment	1936:1974	the immune-suppressive microenvironment of TNBC	1936:1982	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	9	96	theme	oxide	1272:1276	arg1	iNOS					1288:1291	iNOS	1288:1291	iNOS	1288:1291	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	96	theme	oxide	1272:1276	arg1	synthase					1278:1285	inducible nitric oxide synthase	1255:1285	inducible nitric oxide synthase (iNOS)	1255:1292	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	3	97	theme	versatile	567:575	arg1	biomaterials					577:588	versatile biomaterials	567:588	versatile biomaterials with interesting immunomodulatory properties	567:633	Chitosan and lecithin are examples of versatile biomaterials with interesting immunomodulatory properties.
36541457	7	98	theme	Glycolytic	1011:1020	arg1	metabolism					1022:1031	Glycolytic metabolism	1011:1031	Glycolytic metabolism	1011:1031	Glycolytic metabolism was quantified by measuring glucose consumption, adenosine triphosphate (ATP) generation, lactate production and extracellular acidification.
36541457	12	99	theme	immune-suppressive	1940:1957	arg1	microenvironment					1959:1974	the immune-suppressive microenvironment	1936:1974	the immune-suppressive microenvironment of TNBC	1936:1982	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	11	100	theme	negative	1800:1807	arg1	impact					1809:1814	the positive or negative impact	1784:1814	the positive or negative impact on the immunosuppressive characteristics of TNBC cells	1784:1869	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	9	101	theme	quantitative	1466:1477	arg1	q-RT-PCR					1528:1535	q-RT-PCR	1528:1535	q-RT-PCR	1528:1535	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	101	theme	quantitative	1466:1477	arg1	reaction					1518:1525	quantitative reverse transcription polymerase chain reaction	1466:1525	quantitative reverse transcription polymerase chain reaction (q-RT-PCR)	1466:1536	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	12	102	theme	hybrid	1893:1898	arg1	NPs					1918:1920	Carefully engineered hybrid lecithin-chitosan NPs	1872:1920	Carefully engineered hybrid lecithin-chitosan NPs	1872:1920	Carefully engineered hybrid lecithin-chitosan NPs could convert the immune-suppressive microenvironment of TNBC to an immune-active microenvironment via reduction of PD-L1 expression and reversal of the Warburg effect.
36541457	4	103	theme	designed	702:709	arg1	NPs					733:735	NPs	733:735	NPs	733:735	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	4	103	theme	designed	702:709	arg1	nanoparticles					718:730	carefully designed hybrid nanoparticles	692:730	carefully designed hybrid nanoparticles (NPs)	692:736	In this study, we aimed at investigation of the role of carefully designed hybrid nanoparticles (NPs) on common mediators of both programmed death ligand 1 (PD-L1) expression and glycolytic metabolism.
36541457	11	104	theme	systems	1726:1732	arg1	composition					1700:1710	The composition	1696:1710	The composition of the hybrid systems	1696:1732	The composition of the hybrid systems dictated their subcellular localization and hence the positive or negative impact on the immunosuppressive characteristics of TNBC cells.
36541457	1	105	theme	triple-negative	278:292	arg1	cancer					301:306	triple-negative breast cancer	278:306	triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy	278:381	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	1	105	theme	triple-negative	278:292	arg1	TNBC					309:312	TNBC	309:312	TNBC	309:312	Metabolic reprogramming 'Warburg effect' and immune checkpoint signaling are immunosuppressive hallmarks of triple-negative breast cancer (TNBC) contributing to the limited clinical applicability of immunotherapy.
36541457	3	106	theme	immunomodulatory	607:622	arg1	properties					624:633	interesting immunomodulatory properties	595:633	interesting immunomodulatory properties	595:633	Chitosan and lecithin are examples of versatile biomaterials with interesting immunomodulatory properties.
36541457	9	107	theme	transcription	1487:1499	arg1	q-RT-PCR					1528:1535	q-RT-PCR	1528:1535	q-RT-PCR	1528:1535	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36541457	9	107	theme	transcription	1487:1499	arg1	reaction					1518:1525	quantitative reverse transcription polymerase chain reaction	1466:1525	quantitative reverse transcription polymerase chain reaction (q-RT-PCR)	1466:1536	Gene expression of inducible nitric oxide synthase (iNOS), phosphatidylinositol-3-kinase (PI3K), protein kinase B (PKB or Akt), mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α(HIF-1α) and PD-L1 was quantified by quantitative reverse transcription polymerase chain reaction (q-RT-PCR).
36430484	2	0	theme	ozone	349:353	arg1	effect					339:344	The antibacterial effect	321:344	The antibacterial effect of ozone	321:353	The antibacterial effect of ozone is widely known and used, but the instability the gas causes, severely limits its application.
36430484	9	1	theme	keratinocyte	1654:1665	arg1	model					1667:1671	the HaCat keratinocyte model	1644:1671	the HaCat keratinocyte model	1644:1671	The studies proved that the obtained emulsions were rheologically stable, exhibited an antimicrobial effect and did not show cytotoxicity in the HaCat keratinocyte model.
36430484	5	2	with	polymer	765:771	arg1	ingredients					831:841	biologically active ingredients	811:841	biologically active ingredients	811:841	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	5	2	with	polymer	765:771	arg1	capacity					798:805	a high regenerative capacity	778:805	a high regenerative capacity	778:805	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	4	3	contain	containing	676:685	arg2	micro/nanocapsules					687:704	micro/nanocapsules	687:704	micro/nanocapsules of ozonated olive oil	687:726	In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil.
36430484	4	3	contain	containing	676:685	arg1	acid					671:674	hyaluronic acid	660:674	hyaluronic acid containing micro/nanocapsules of ozonated olive oil	660:726	In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil.
36430484	3	4	from	entrapment	456:465	arg1	oil					476:478	olive oil	470:478	olive oil	470:478	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	3	5	theme	unsaturated	504:514	arg1	bond					516:519	an unsaturated bond	501:519	an unsaturated bond	501:519	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	3	6	dep	stable	550:555	arg1	active					574:579	active	574:579	active	574:579	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	4	7	theme	innovative	630:639	arg1	hydrogel					641:648	an innovative hydrogel	627:648	an innovative hydrogel	627:648	In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil.
36430484	6	8	theme	electron	1139:1146	arg1	microscopy					1148:1157	scanning electron microscopy	1130:1157	scanning electron microscopy (SEM)	1130:1163	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	6	8	theme	electron	1139:1146	arg1	SEM					1160:1162	SEM	1160:1162	SEM	1160:1162	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	5	9	with	effect	923:928	arg1	properties					885:894	regenerative properties	872:894	regenerative properties	872:894	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	1	10	theme	natural	203:209	arg1	polymers					211:218	natural polymers	203:218	natural polymers	203:218	Hydrogels, based on natural polymers, such as hyaluronic acid, are gaining an increasing popularity because of their biological activity.
36430484	8	11	theme	in	1443:1444	arg1	testing					1452:1458	in vitro testing	1443:1458	in vitro testing	1443:1458	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	6	12	theme	scattering	1204:1213	arg1	method					1221:1226	the dynamic light scattering (DLS) method	1186:1226	the dynamic light scattering (DLS) method	1186:1226	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	3	13	theme	stable	550:555	arg1	derivatives					587:597	stable, therapeutically active ozone derivatives	550:597	stable, therapeutically active ozone derivatives	550:597	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	8	14	dep	analysis	1430:1437	arg1	the					1410:1412	the	1410:1412	the	1410:1412	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	6	15	theme	dynamic	1190:1196	arg1	scattering					1204:1213	dynamic light scattering	1190:1213	the dynamic light scattering (DLS) method	1186:1226	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	6	15	theme	dynamic	1190:1196	arg1	DLS					1216:1218	DLS	1216:1218	DLS	1216:1218	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	4	16	theme	oil	724:726	arg1	micro/nanocapsules					687:704	micro/nanocapsules	687:704	micro/nanocapsules of ozonated olive oil	687:726	In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil.
36430484	0	17	theme	Obtained	160:167	arg1	Biocomposite					169:180	the Obtained Biocomposite	156:180	the Obtained Biocomposite	156:180	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	6	18	theme	particle	1169:1176	arg1	size					1178:1181	particle size	1169:1181	particle size	1169:1181	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	5	19	theme	weak	907:910	arg1	effect					923:928	a very weak inhibitory effect	900:928	a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts	900:1012	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	8	20	theme	human	1473:1477	arg1	line					1497:1500	the HaCat human keratinocyte cell line	1463:1500	the HaCat human keratinocyte cell line	1463:1500	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	2	21	used	used	375:378	arg2	effect					339:344	The antibacterial effect	321:344	The antibacterial effect of ozone	321:353	The antibacterial effect of ozone is widely known and used, but the instability the gas causes, severely limits its application.
36430484	8	22	from	testing	1452:1458	arg1	line					1497:1500	the HaCat human keratinocyte cell line	1463:1500	the HaCat human keratinocyte cell line	1463:1500	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	3	23	theme	ozone	581:585	arg1	derivatives					587:597	stable, therapeutically active ozone derivatives	550:597	stable, therapeutically active ozone derivatives	550:597	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	0	24	from	Nano/Microcapsules	15:32	arg1	Matrix					70:75	Hyaluronan Matrix	59:75	Hyaluronan Matrix	59:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	5	25	with	hydrogel	858:865	arg1	properties					885:894	regenerative properties	872:894	regenerative properties	872:894	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	5	26	theme	commensal	953:961	arg1	microbiota					968:977	both bacterial commensal skin microbiota and pathogenic Candida-like yeasts	938:1012	microbiota	968:977	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	0	27	from	Oil	52:54	arg1	Matrix					70:75	Hyaluronan Matrix	59:75	Hyaluronan Matrix	59:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	6	28	theme	gel	1075:1077	arg1	stability					1031:1039	stability	1031:1039	stability	1031:1039	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	6	28	theme	gel	1075:1077	arg1	properties					1057:1066	rheological properties	1045:1066	rheological properties	1045:1066	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	4	29	theme	ozonated	709:716	arg1	oil					724:726	ozonated olive oil	709:726	ozonated olive oil	709:726	In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil.
36430484	5	30	theme	high	780:783	arg1	capacity					798:805	a high regenerative capacity	778:805	a high regenerative capacity	778:805	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	1	31	theme	increasing	261:270	arg1	popularity					272:281	an increasing popularity	258:281	an increasing popularity	258:281	Hydrogels, based on natural polymers, such as hyaluronic acid, are gaining an increasing popularity because of their biological activity.
36430484	7	32	theme	FTIR-ATR	1304:1311	arg1	spectroscopy					1314:1325	(FTIR-ATR) spectroscopy	1303:1325	(FTIR-ATR) spectroscopy	1303:1325	We also performed Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy.
36430484	8	33	theme	antimicrobial	1369:1381	arg1	potential					1383:1391	the antimicrobial potential	1365:1391	the antimicrobial potential	1365:1391	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	2	34	theme	gas	405:407	arg1	causes					409:414	the gas causes	401:414	the gas causes	401:414	The antibacterial effect of ozone is widely known and used, but the instability the gas causes, severely limits its application.
36430484	0	35	theme	Nano/Microcapsules	15:32	arg1	Preparation					0:10	Preparation	0:10	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix	0:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	0	35	theme	Nano/Microcapsules	15:32	arg1	Analysis					81:88	Analysis	81:88	Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite	81:180	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	6	36	theme	rheological	1045:1055	arg1	properties					1057:1066	rheological properties	1045:1066	rheological properties	1045:1066	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	2	37	dep	instability	389:399	arg1	causes					409:414	the gas causes	401:414	the gas causes	401:414	The antibacterial effect of ozone is widely known and used, but the instability the gas causes, severely limits its application.
36430484	0	38	theme	Ozonated	37:44	arg1	Oil					52:54	Ozonated Olive Oil	37:54	Ozonated Olive Oil in Hyaluronan Matrix	37:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	5	39	theme	biocompatible	751:763	arg1	polymer					765:771	the biocompatible polymer	747:771	the biocompatible polymer with a high regenerative capacity and biologically active ingredients	747:841	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	0	40	theme	Hyaluronan	59:68	arg1	Matrix					70:75	Hyaluronan Matrix	59:75	Hyaluronan Matrix	59:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	3	41	theme	olive	470:474	arg1	oil					476:478	olive oil	470:478	olive oil	470:478	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	0	42	theme	Oil	52:54	arg1	Nano/Microcapsules					15:32	Nano/Microcapsules	15:32	Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix	15:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	7	43	theme	total	1258:1262	arg1	Fourier					1276:1282	Attenuated total reflectance Fourier	1247:1282	Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy	1247:1325	We also performed Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy.
36430484	8	44	theme	microbiological	1414:1428	arg1	analysis					1430:1437	microbiological analysis	1414:1437	microbiological analysis	1414:1437	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	2	45	theme	antibacterial	325:337	arg1	effect					339:344	The antibacterial effect	321:344	The antibacterial effect of ozone	321:353	The antibacterial effect of ozone is widely known and used, but the instability the gas causes, severely limits its application.
36430484	7	46	dep	Fourier	1276:1282	arg1	transform					1284:1292	transform	1284:1292	transform infrared (FTIR-ATR) spectroscopy	1284:1325	We also performed Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy.
36430484	1	47	theme	hyaluronic	229:238	arg1	acid					240:243	hyaluronic acid	229:243	hyaluronic acid	229:243	Hydrogels, based on natural polymers, such as hyaluronic acid, are gaining an increasing popularity because of their biological activity.
36430484	8	48	theme	cell	1492:1495	arg1	line					1497:1500	the HaCat human keratinocyte cell line	1463:1500	the HaCat human keratinocyte cell line	1463:1500	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	9	49	theme	HaCat	1648:1652	arg1	model					1667:1671	the HaCat keratinocyte model	1644:1671	the HaCat keratinocyte model	1644:1671	The studies proved that the obtained emulsions were rheologically stable, exhibited an antimicrobial effect and did not show cytotoxicity in the HaCat keratinocyte model.
36430484	3	50	with	reaction	487:494	arg1	bond					516:519	an unsaturated bond	501:519	an unsaturated bond	501:519	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	0	51	theme	Biological	130:139	arg1	Properties					142:151	Physicochemical and Microbiological (Biological) Properties	93:151	Properties	142:151	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	8	52	from	analysis	1430:1437	arg1	line					1497:1500	the HaCat human keratinocyte cell line	1463:1500	the HaCat human keratinocyte cell line	1463:1500	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	6	53	theme	scanning	1130:1137	arg1	microscopy					1148:1157	scanning electron microscopy	1130:1157	scanning electron microscopy (SEM)	1130:1163	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	6	53	theme	scanning	1130:1137	arg1	SEM					1160:1162	SEM	1160:1162	SEM	1160:1162	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	0	54	theme	Properties	142:151	arg1	Preparation					0:10	Preparation	0:10	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix	0:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	0	54	theme	Properties	142:151	arg1	Analysis					81:88	Analysis	81:88	Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite	81:180	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	5	55	theme	active	824:829	arg1	ingredients					831:841	biologically active ingredients	811:841	biologically active ingredients	811:841	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	0	56	from	Matrix	70:75	arg1	Nano/Microcapsules					15:32	Nano/Microcapsules	15:32	Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix	15:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	8	57	theme	HaCat	1467:1471	arg1	line					1497:1500	the HaCat human keratinocyte cell line	1463:1500	the HaCat human keratinocyte cell line	1463:1500	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	6	58	theme	light	1198:1202	arg1	scattering					1204:1213	dynamic light scattering	1190:1213	the dynamic light scattering (DLS) method	1186:1226	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	6	58	theme	light	1198:1202	arg1	DLS					1216:1218	DLS	1216:1218	DLS	1216:1218	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	4	59	theme	hyaluronic	660:669	arg1	acid					671:674	hyaluronic acid	660:674	hyaluronic acid containing micro/nanocapsules of ozonated olive oil	660:726	In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil.
36430484	0	60	theme	Biocomposite	169:180	arg1	Properties					142:151	Physicochemical and Microbiological (Biological) Properties	93:151	Properties	142:151	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	3	61	theme	derivatives	587:597	arg1	formation					537:545	the formation	533:545	the formation of stable, therapeutically active ozone derivatives	533:597	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	8	62	theme	keratinocyte	1479:1490	arg1	line					1497:1500	the HaCat human keratinocyte cell line	1463:1500	the HaCat human keratinocyte cell line	1463:1500	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	5	63	theme	bacterial	943:951	arg1	microbiota					968:977	both bacterial commensal skin microbiota and pathogenic Candida-like yeasts	938:1012	microbiota	968:977	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	5	64	theme	regenerative	872:883	arg1	properties					885:894	regenerative properties	872:894	regenerative properties	872:894	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	0	65	theme	Physicochemical	93:107	arg1	Properties					142:151	Physicochemical and Microbiological (Biological) Properties	93:151	Properties	142:151	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	5	66	theme	pathogenic	983:992	arg1	yeasts					1007:1012	both bacterial commensal skin microbiota and pathogenic Candida-like yeasts	938:1012	yeasts	1007:1012	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	9	67	theme	obtained	1531:1538	arg1	emulsions					1540:1548	the obtained emulsions	1527:1548	the obtained emulsions	1527:1548	The studies proved that the obtained emulsions were rheologically stable, exhibited an antimicrobial effect and did not show cytotoxicity in the HaCat keratinocyte model.
36430484	9	67	theme	obtained	1531:1538	arg1	stable					1569:1574	stable	1569:1574	stable	1569:1574	The studies proved that the obtained emulsions were rheologically stable, exhibited an antimicrobial effect and did not show cytotoxicity in the HaCat keratinocyte model.
36430484	6	68	dep	stability	1031:1039	arg1	the					1027:1029	the	1027:1029	the	1027:1029	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	0	69	theme	Microbiological	113:127	arg1	Properties					142:151	Physicochemical and Microbiological (Biological) Properties	93:151	Properties	142:151	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	5	70	theme	skin	963:966	arg1	microbiota					968:977	both bacterial commensal skin microbiota and pathogenic Candida-like yeasts	938:1012	microbiota	968:977	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	8	71	theme	functional	1332:1341	arg1	potential					1383:1391	the antimicrobial potential	1365:1391	the antimicrobial potential	1365:1391	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	8	71	theme	functional	1332:1341	arg1	properties					1343:1352	The functional properties	1328:1352	The functional properties	1328:1352	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	5	72	theme	inhibitory	912:921	arg1	effect					923:928	a very weak inhibitory effect	900:928	a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts	900:1012	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	5	73	theme	regenerative	785:796	arg1	capacity					798:805	a high regenerative capacity	778:805	a high regenerative capacity	778:805	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	5	74	theme	Candida-like	994:1005	arg1	yeasts					1007:1012	both bacterial commensal skin microbiota and pathogenic Candida-like yeasts	938:1012	yeasts	1007:1012	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	4	75	theme	olive	718:722	arg1	oil					724:726	ozonated olive oil	709:726	ozonated olive oil	709:726	In this study, we obtained an innovative hydrogel, based on hyaluronic acid containing micro/nanocapsules of ozonated olive oil.
36430484	3	76	theme	Ozone	450:454	arg1	entrapment					456:465	Ozone entrapment	450:465	Ozone entrapment	450:465	Ozone entrapment in olive oil by its reaction with an unsaturated bond, allows for the formation of stable, therapeutically active ozone derivatives.
36430484	0	77	theme	Olive	46:50	arg1	Oil					52:54	Ozonated Olive Oil	37:54	Ozonated Olive Oil in Hyaluronan Matrix	37:75	Preparation of Nano/Microcapsules of Ozonated Olive Oil in Hyaluronan Matrix and Analysis of Physicochemical and Microbiological (Biological) Properties of the Obtained Biocomposite.
36430484	9	78	theme	antimicrobial	1590:1602	arg1	effect					1604:1609	an antimicrobial effect	1587:1609	an antimicrobial effect	1587:1609	The studies proved that the obtained emulsions were rheologically stable, exhibited an antimicrobial effect and did not show cytotoxicity in the HaCat keratinocyte model.
36430484	7	79	theme	reflectance	1264:1274	arg1	Fourier					1276:1282	Attenuated total reflectance Fourier	1247:1282	Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy	1247:1325	We also performed Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy.
36430484	5	80	theme	polymer	765:771	arg1	combination					732:742	combination	732:742	combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients	732:841	By combination of the biocompatible polymer with a high regenerative capacity and biologically active ingredients, we obtained a hydrogel with regenerative properties and a very weak inhibitory effect against both bacterial commensal skin microbiota and pathogenic Candida-like yeasts.
36430484	1	81	theme	biological	300:309	arg1	activity					311:318	their biological activity	294:318	their biological activity	294:318	Hydrogels, based on natural polymers, such as hyaluronic acid, are gaining an increasing popularity because of their biological activity.
36430484	7	82	theme	Attenuated	1247:1256	arg1	Fourier					1276:1282	Attenuated total reflectance Fourier	1247:1282	Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy	1247:1325	We also performed Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy.
36430484	8	83	dep	in	1443:1444	arg1	vitro					1446:1450	vitro	1446:1450	vitro	1446:1450	The functional properties, including the antimicrobial potential were assessed by the microbiological analysis and in vitro testing on the HaCat human keratinocyte cell line.
36430484	6	84	theme	composite	1113:1121	arg1	morphology					1095:1104	the morphology	1091:1104	the morphology of the composite	1091:1121	We assessed the stability and rheological properties of the gel, determined the morphology of the composite, using scanning electron microscopy (SEM) and particle size by the dynamic light scattering (DLS) method.
36430484	7	85	dep	transform	1284:1292	arg1	infrared					1294:1301	infrared	1294:1301	transform infrared (FTIR-ATR) spectroscopy	1284:1325	We also performed Attenuated total reflectance Fourier transform infrared (FTIR-ATR) spectroscopy.
35744991	6	0	theme	Taste	927:931	arg1	differences					946:956	Taste and nutrient differences	927:956	Taste and nutrient differences	927:956	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	7	1	theme	chemical	1125:1132	arg1	characterization					1134:1149	a comprehensive chemical characterization	1109:1149	a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits	1109:1213	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	6	2	theme	acids	1061:1065	arg1	abundance					1009:1017	abundance	1009:1017	abundance	1009:1017	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	6	2	theme	acids	1061:1065	arg1	composition					993:1003	composition	993:1003	composition	993:1003	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	5	3	theme	organic	900:906	arg1	biosynthesis					913:924	the sugar and organic acid biosynthesis	886:924	biosynthesis	913:924	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	7	4	from	compounds	1173:1181	arg1	apricot					1199:1205	apricot	1199:1205	apricot	1199:1205	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	7	5	from	taste	1154:1158	arg1	apricot					1199:1205	apricot	1199:1205	apricot	1199:1205	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	0	6	from	Identification	15:28	arg1	Apricot					86:92	Apricot	86:92	Apricot	86:92	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	1	7	theme	content	158:164	arg1	variation					166:174	the content variation	154:174	the content variation in taste substances and nutrients in 'Shushanggan apricot'	154:233	The chemotypic and the content variation in taste substances and nutrients in 'Shushanggan apricot' fruits were detected by UPLC-MS/MS.
35744991	7	8	from	apricot	1199:1205	arg1	characterization					1134:1149	a comprehensive chemical characterization	1109:1149	a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits	1109:1213	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	7	9	theme	compounds	1173:1181	arg1	characterization					1134:1149	a comprehensive chemical characterization	1109:1149	a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits	1109:1213	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	3	10	theme	dominant	518:525	arg1	acids					538:542	the dominant free fatty acids	514:542	the dominant free fatty acids	514:542	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	10	theme	dominant	518:525	arg1	acid					468:471	γ-linolenic acid	456:471	γ-linolenic acid	456:471	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	10	theme	dominant	518:525	arg1	acid					486:489	α-linolenic acid	474:489	α-linolenic acid	474:489	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	10	theme	dominant	518:525	arg1	acid					504:507	linoleic acid	495:507	linoleic acid	495:507	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	6	11	theme	amino	1055:1059	arg1	acids					1061:1065	amino acids	1055:1065	amino acids	1055:1065	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	0	12	from	Components	59:68	arg1	Apricot					86:92	Apricot	86:92	Apricot	86:92	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	3	13	theme	fatty	532:536	arg1	acids					538:542	the dominant free fatty acids	514:542	the dominant free fatty acids	514:542	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	13	theme	fatty	532:536	arg1	acid					468:471	γ-linolenic acid	456:471	γ-linolenic acid	456:471	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	13	theme	fatty	532:536	arg1	acid					486:489	α-linolenic acid	474:489	α-linolenic acid	474:489	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	13	theme	fatty	532:536	arg1	acid					504:507	linoleic acid	495:507	linoleic acid	495:507	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	14	theme	free	527:530	arg1	acids					538:542	the dominant free fatty acids	514:542	the dominant free fatty acids	514:542	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	14	theme	free	527:530	arg1	acid					468:471	γ-linolenic acid	456:471	γ-linolenic acid	456:471	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	14	theme	free	527:530	arg1	acid					486:489	α-linolenic acid	474:489	α-linolenic acid	474:489	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	14	theme	free	527:530	arg1	acid					504:507	linoleic acid	495:507	linoleic acid	495:507	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	5	15	theme	pathways	865:872	arg1	biosynthesis					801:812	the biosynthesis	797:812	the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways	797:872	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	5	16	theme	2-oxocarboxylic	833:847	arg1	pathways					865:872	2-oxocarboxylic acid metabolism pathways	833:872	2-oxocarboxylic acid metabolism pathways	833:872	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	2	17	theme	malic	372:376	arg1	acid					378:381	malic acid	372:381	malic acid	372:381	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	7	18	theme	taste	1154:1158	arg1	characterization					1134:1149	a comprehensive chemical characterization	1109:1149	a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits	1109:1213	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	5	19	theme	acid	908:911	arg1	biosynthesis					913:924	the sugar and organic acid biosynthesis	886:924	biosynthesis	913:924	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	7	20	dep	taste	1154:1158	arg1	fruits					1208:1213	fruits	1208:1213	fruits	1208:1213	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	2	21	theme	organic	414:420	arg1	acids					422:426	important organic acids	404:426	important organic acids affecting sweet-sour taste	404:453	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	7	22	theme	study	1088:1092	arg1	purpose					1072:1078	The purpose	1068:1078	The purpose of this study	1068:1092	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	4	23	theme	citric	724:729	arg1	acid					731:734	citric acid	724:734	citric acid	724:734	Fruit taste was positively correlated with sucrose and negatively correlated with malic acid and citric acid.
35744991	1	24	theme	taste	179:183	arg1	substances					185:194	taste substances	179:194	taste substances	179:194	The chemotypic and the content variation in taste substances and nutrients in 'Shushanggan apricot' fruits were detected by UPLC-MS/MS.
35744991	0	25	theme	Chemotaxonomic	0:13	arg1	Identification					15:28	Chemotaxonomic Identification	0:28	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot'	0:93	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	7	26	theme	nutrient	1164:1171	arg1	compounds					1173:1181	nutrient compounds	1164:1181	nutrient compounds in 'Shushanggan apricot'	1164:1206	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	6	27	theme	nutrient	937:944	arg1	differences					946:956	Taste and nutrient differences	927:956	Taste and nutrient differences	927:956	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	2	28	theme	sweet	356:360	arg1	taste					362:366	the sweet taste	352:366	the sweet taste	352:366	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	0	29	theme	Key	33:35	arg1	Components					59:68	Key Taste and Nutritional Components	33:68	Key Taste and Nutritional Components in 'Shushanggan Apricot'	33:93	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	7	30	theme	comprehensive	1111:1123	arg1	characterization					1134:1149	a comprehensive chemical characterization	1109:1149	a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits	1109:1213	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	2	31	theme	compounds	286:294	arg1	total					273:277	A total	271:277	A total of 592 compounds	271:294	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	5	32	theme	acids	823:827	arg1	biosynthesis					801:812	the biosynthesis	797:812	the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways	797:872	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	0	33	theme	Targeted	112:119	arg1	Metabolomics					121:132	Widely Targeted Metabolomics	105:132	Widely Targeted Metabolomics	105:132	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	0	34	from	Apricot	86:92	arg1	Identification					15:28	Chemotaxonomic Identification	0:28	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot'	0:93	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	2	35	theme	592	282:284	arg1	compounds					286:294	592 compounds	282:294	592 compounds	282:294	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	2	36	theme	citric	387:392	arg1	acid					394:397	citric acid	387:397	citric acid	387:397	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	5	37	theme	acid	849:852	arg1	pathways					865:872	2-oxocarboxylic acid metabolism pathways	833:872	2-oxocarboxylic acid metabolism pathways	833:872	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	4	38	theme	Fruit	627:631	arg1	taste					633:637	Fruit taste	627:637	Fruit taste	627:637	Fruit taste was positively correlated with sucrose and negatively correlated with malic acid and citric acid.
35744991	0	39	theme	Taste	37:41	arg1	Components					59:68	Key Taste and Nutritional Components	33:68	Key Taste and Nutritional Components in 'Shushanggan Apricot'	33:93	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	6	40	from	variations	979:988	arg1	abundance					1009:1017	abundance	1009:1017	abundance	1009:1017	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	6	40	from	variations	979:988	arg1	composition					993:1003	composition	993:1003	composition	993:1003	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	3	41	theme	phenolic	611:618	arg1	acids					620:624	the predominant phenolic acids	595:624	the predominant phenolic acids	595:624	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	41	theme	phenolic	611:618	arg1	acid					585:588	chlorogenic acid	573:588	chlorogenic acid	573:588	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	41	theme	phenolic	611:618	arg1	acid					564:567	neochlorogenic acid	549:567	neochlorogenic acid	549:567	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	2	42	theme	important	404:412	arg1	acids					422:426	important organic acids	404:426	important organic acids affecting sweet-sour taste	404:453	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	5	43	theme	amino	817:821	arg1	acids					823:827	amino acids	817:827	amino acids	817:827	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	0	44	theme	Components	59:68	arg1	Identification					15:28	Chemotaxonomic Identification	0:28	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot'	0:93	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	5	45	theme	differential	741:752	arg1	metabolites					754:764	The differential metabolites	737:764	The differential metabolites	737:764	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	3	46	theme	neochlorogenic	549:562	arg1	acid					564:567	neochlorogenic acid	549:567	neochlorogenic acid	549:567	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	46	theme	neochlorogenic	549:562	arg1	acid					585:588	chlorogenic acid	573:588	chlorogenic acid	573:588	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	46	theme	neochlorogenic	549:562	arg1	acids					620:624	the predominant phenolic acids	595:624	the predominant phenolic acids	595:624	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	7	47	from	characterization	1134:1149	arg1	apricot					1199:1205	apricot	1199:1205	apricot	1199:1205	The purpose of this study was to provide a comprehensive chemical characterization of taste and nutrient compounds in 'Shushanggan apricot' fruits.
35744991	0	48	theme	Nutritional	47:57	arg1	Components					59:68	Key Taste and Nutritional Components	33:68	Key Taste and Nutritional Components in 'Shushanggan Apricot'	33:93	Chemotaxonomic Identification of Key Taste and Nutritional Components in 'Shushanggan Apricot' Fruits by Widely Targeted Metabolomics.
35744991	3	49	theme	α-linolenic	474:484	arg1	acids					538:542	the dominant free fatty acids	514:542	the dominant free fatty acids	514:542	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	49	theme	α-linolenic	474:484	arg1	acid					468:471	γ-linolenic acid	456:471	γ-linolenic acid	456:471	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	49	theme	α-linolenic	474:484	arg1	acid					486:489	α-linolenic acid	474:489	α-linolenic acid	474:489	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	49	theme	α-linolenic	474:484	arg1	acid					504:507	linoleic acid	495:507	linoleic acid	495:507	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	6	50	theme	organic	1037:1043	arg1	acids					1045:1049	organic acids	1037:1049	organic acids	1037:1049	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	3	51	theme	chlorogenic	573:583	arg1	acids					620:624	the predominant phenolic acids	595:624	the predominant phenolic acids	595:624	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	51	theme	chlorogenic	573:583	arg1	acid					585:588	chlorogenic acid	573:588	chlorogenic acid	573:588	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	51	theme	chlorogenic	573:583	arg1	acid					564:567	neochlorogenic acid	549:567	neochlorogenic acid	549:567	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	1	52	from	nutrients	200:208	arg1	apricot					226:232	apricot	226:232	apricot	226:232	The chemotypic and the content variation in taste substances and nutrients in 'Shushanggan apricot' fruits were detected by UPLC-MS/MS.
35744991	2	53	dep	identified	301:310	arg1	acids					422:426	important organic acids	404:426	important organic acids affecting sweet-sour taste	404:453	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	2	53	dep	identified	301:310	arg1	contributed					330:340	contributed	330:340	contributed mainly to the sweet taste	330:366	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	1	54	dep	chemotypic	139:148	arg1	fruits					235:240	fruits	235:240	fruits	235:240	The chemotypic and the content variation in taste substances and nutrients in 'Shushanggan apricot' fruits were detected by UPLC-MS/MS.
35744991	6	55	theme	acids	1045:1049	arg1	abundance					1009:1017	abundance	1009:1017	abundance	1009:1017	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	6	55	theme	acids	1045:1049	arg1	composition					993:1003	composition	993:1003	composition	993:1003	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	3	56	theme	predominant	599:609	arg1	acids					620:624	the predominant phenolic acids	595:624	the predominant phenolic acids	595:624	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	56	theme	predominant	599:609	arg1	acid					585:588	chlorogenic acid	573:588	chlorogenic acid	573:588	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	56	theme	predominant	599:609	arg1	acid					564:567	neochlorogenic acid	549:567	neochlorogenic acid	549:567	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	4	57	theme	malic	709:713	arg1	acid					715:718	malic acid	709:718	malic acid	709:718	Fruit taste was positively correlated with sucrose and negatively correlated with malic acid and citric acid.
35744991	3	58	theme	γ-linolenic	456:466	arg1	acids					538:542	the dominant free fatty acids	514:542	the dominant free fatty acids	514:542	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	58	theme	γ-linolenic	456:466	arg1	acid					468:471	γ-linolenic acid	456:471	γ-linolenic acid	456:471	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	58	theme	γ-linolenic	456:466	arg1	acid					486:489	α-linolenic acid	474:489	α-linolenic acid	474:489	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	58	theme	γ-linolenic	456:466	arg1	acid					504:507	linoleic acid	495:507	linoleic acid	495:507	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	5	59	theme	metabolism	854:863	arg1	pathways					865:872	2-oxocarboxylic acid metabolism pathways	833:872	2-oxocarboxylic acid metabolism pathways	833:872	The differential metabolites were significantly enriched in the biosynthesis of amino acids and 2-oxocarboxylic acid metabolism pathways, regulating the sugar and organic acid biosynthesis.
35744991	1	60	from	substances	185:194	arg1	apricot					226:232	apricot	226:232	apricot	226:232	The chemotypic and the content variation in taste substances and nutrients in 'Shushanggan apricot' fruits were detected by UPLC-MS/MS.
35744991	3	61	theme	linoleic	495:502	arg1	acids					538:542	the dominant free fatty acids	514:542	the dominant free fatty acids	514:542	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	61	theme	linoleic	495:502	arg1	acid					468:471	γ-linolenic acid	456:471	γ-linolenic acid	456:471	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	61	theme	linoleic	495:502	arg1	acid					486:489	α-linolenic acid	474:489	α-linolenic acid	474:489	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	3	61	theme	linoleic	495:502	arg1	acid					504:507	linoleic acid	495:507	linoleic acid	495:507	γ-linolenic acid, α-linolenic acid and linoleic acid were the dominant free fatty acids, and neochlorogenic acid and chlorogenic acid were the predominant phenolic acids.
35744991	2	62	theme	sweet-sour	438:447	arg1	taste					449:453	sweet-sour taste	438:453	sweet-sour taste	438:453	A total of 592 compounds were identified, of which sucrose contributed mainly to the sweet taste and malic acid and citric acid were important organic acids affecting sweet-sour taste.
35744991	1	63	from	variation	166:174	arg1	nutrients					200:208	nutrients	200:208	nutrients	200:208	The chemotypic and the content variation in taste substances and nutrients in 'Shushanggan apricot' fruits were detected by UPLC-MS/MS.
35744991	1	63	from	variation	166:174	arg1	substances					185:194	taste substances	179:194	taste substances	179:194	The chemotypic and the content variation in taste substances and nutrients in 'Shushanggan apricot' fruits were detected by UPLC-MS/MS.
35744991	6	64	theme	carbohydrates	1022:1034	arg1	abundance					1009:1017	abundance	1009:1017	abundance	1009:1017	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
35744991	6	64	theme	carbohydrates	1022:1034	arg1	composition					993:1003	composition	993:1003	composition	993:1003	Taste and nutrient differences could be revealed by variations in composition and abundance of carbohydrates, organic acids and amino acids.
36569878	11	0	theme	chronic	2456:2462	arg1	state					2477:2481	the chronic inflammatory state	2452:2481	the chronic inflammatory state leading to low dietary fiber-related diseases in animals	2452:2538	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	3	1	theme	gut	728:730	arg1	architecture					743:754	gut microbiota architecture	728:754	gut microbiota architecture	728:754	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	1	2	theme	barrier	298:304	arg1	functions					306:314	the intestinal barrier functions	283:314	the intestinal barrier functions	283:314	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	10	3	theme	meat	2381:2384	arg1	geese					2386:2390	AGF meat geese	2377:2390	AGF meat geese	2377:2390	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	9	4	theme	top	1966:1968	arg1	markers					1978:1984	top 44 host markers	1966:1984	top 44 host markers with gut microbiota	1966:2004	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	8	5	theme	significant	1738:1748	arg1	increase					1750:1757	A significant increase	1736:1757	A significant increase in intestinal ALP-induced Nrf2 signaling pathway	1736:1806	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	7	6	dep	showed	1538:1543	arg1	compared					1614:1621	compared	1614:1621	showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system	1538:1733	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	7	from	increase	1559:1566	arg1	richness					1591:1598	commensal microbial richness	1571:1598	commensal microbial richness	1571:1598	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	8	theme	microbiota	1480:1489	arg1	analysis					1491:1498	the gut microbiota analysis	1472:1498	the gut microbiota analysis	1472:1498	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	8	theme	microbiota	1480:1489	arg1	geese					1506:1510	meat geese	1501:1510	meat geese supplemented with pasture	1501:1536	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	6	9	theme	protein	1211:1217	arg1	expression					1219:1228	protein expression	1211:1228	protein expression of serum and cecal tissues	1211:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	9	10	theme	host	1973:1976	arg1	markers					1978:1984	top 44 host markers	1966:1984	top 44 host markers with gut microbiota	1966:2004	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	5	11	dep	IHF	1076:1078	arg1	systems					1088:1094	systems	1088:1094	systems	1088:1094	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	3	12	theme	gut	760:762	arg1	functions					772:780	gut barrier functions	760:780	gut barrier functions	760:780	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	2	13	theme	In-house	373:380	arg1	system					390:395	In-house feeding system	373:395	In-house feeding system (IHF, a low dietary fiber source)	373:429	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	8	14	theme	ALP-induced	1773:1783	arg1	pathway					1800:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	1	15	theme	microbiota	228:237	arg1	composition					239:249	gut microbiota composition	224:249	gut microbiota composition	224:249	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	11	16	theme	low	2494:2496	arg1	diseases					2520:2527	low dietary fiber-related diseases	2494:2527	low dietary fiber-related diseases in animals	2494:2538	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	6	17	theme	host	1181:1184	arg1	analysis					1194:1201	The host markers analysis	1177:1201	The host markers analysis through protein expression of serum and cecal tissues	1177:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	5	18	dep	Methods	1053:1059	arg1	assessed					1153:1160	assessed	1153:1160	were assessed in meat geese	1148:1174	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	11	19	theme	dietary	2498:2504	arg1	diseases					2520:2527	low dietary fiber-related diseases	2494:2527	low dietary fiber-related diseases in animals	2494:2538	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	6	20	theme	western	1359:1365	arg1	analysis					1376:1383	western blotting analysis	1359:1383	western blotting analysis of ALP	1359:1390	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	5	21	theme	microbial	1103:1111	arg1	composition					1113:1123	gut microbial composition	1099:1123	gut microbial composition via 16 sRNA sequencing	1099:1146	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	5	22	from	impacts	1065:1071	arg1	composition					1113:1123	gut microbial composition	1099:1123	gut microbial composition via 16 sRNA sequencing	1099:1146	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	6	23	theme	eosin	1274:1278	arg1	staining					1286:1293	hematoxylin and eosin (H&E) staining	1258:1293	hematoxylin and eosin (H&E) staining	1258:1293	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	11	24	theme	AGF	2397:2399	arg1	system					2401:2406	The AGF system	2393:2406	The AGF system	2393:2406	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	3	25	theme	grazing	664:670	arg1	system					672:677	artificial pasture grazing system	645:677	artificial pasture grazing system (AGF, a high dietary fiber source)	645:712	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	1	26	theme	gut	256:258	arg1	bacteria					260:267	gut bacteria	256:267	gut bacteria	256:267	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	4	27	theme	oxygen	941:946	arg1	species					948:954	reactive oxygen species	932:954	reactive oxygen species (ROS) generation	932:971	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	4	27	theme	oxygen	941:946	arg1	ROS					957:959	ROS	957:959	ROS	957:959	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	6	28	theme	hematoxylin	1258:1268	arg1	staining					1286:1293	hematoxylin and eosin (H&E) staining	1258:1293	hematoxylin and eosin (H&E) staining	1258:1293	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	9	29	theme	pasture	2029:2035	arg1	intake					2037:2042	artificial pasture intake	2018:2042	artificial pasture intake	2018:2042	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	8	30	from	mechanisms	1899:1908	arg1	geese					1922:1926	AGF meat geese	1913:1926	AGF meat geese	1913:1926	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	3	31	theme	meat	812:815	arg1	geese					817:821	meat geese	812:821	meat geese	812:821	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	5	32	theme	AGF	1084:1086	arg1	impacts					1065:1071	The impacts	1061:1071	The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing	1061:1146	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	3	33	theme	artificial	645:654	arg1	system					672:677	artificial pasture grazing system	645:677	artificial pasture grazing system (AGF, a high dietary fiber source)	645:712	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	10	34	theme	LPS-induced	2347:2357	arg1	production					2363:2372	LPS-induced ROS production	2347:2372	LPS-induced ROS production	2347:2372	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	6	35	theme	tissues	1249:1255	arg1	expression					1219:1228	protein expression	1211:1228	protein expression of serum and cecal tissues	1211:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	10	36	theme	microbial	2247:2255	arg1	homeostasis					2257:2267	gut microbial homeostasis	2243:2267	gut microbial homeostasis	2243:2267	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	7	37	theme	IHF	1626:1628	arg1	geese					1635:1639	IHF meat geese	1626:1639	IHF meat geese	1626:1639	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	9	38	theme	barrier	2058:2064	arg1	functions					2066:2074	gut barrier functions	2054:2074	gut barrier functions	2054:2074	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	4	39	theme	meat	1041:1044	arg1	geese					1046:1050	meat geese	1041:1050	meat geese	1041:1050	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	0	40	theme	gut	101:103	arg1	microbiota					105:114	gut microbiota	101:114	gut microbiota	101:114	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	8	41	theme	ROS	1885:1887	arg1	mechanisms					1899:1908	TLR4/MyD88 induced ROS reduction mechanisms	1866:1908	TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese	1866:1926	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	2	42	theme	alkaline	591:598	arg1	ALP					613:615	ALP	613:615	ALP	613:615	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	2	42	theme	alkaline	591:598	arg1	phosphatase					600:610	intestinal alkaline phosphatase	580:610	reduced intestinal alkaline phosphatase (ALP) production	572:627	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	9	43	theme	ROS-mediated	2089:2100	arg1	permeability					2128:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	6	44	theme	ALP	1388:1390	arg1	staining					1286:1293	hematoxylin and eosin (H&E) staining	1258:1293	hematoxylin and eosin (H&E) staining	1258:1293	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	44	theme	ALP	1388:1390	arg1	localization					1296:1307	localization	1296:1307	localization of NF-қB and Nrf2 by immunofluorescence analysis	1296:1356	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	44	theme	ALP	1388:1390	arg1	analysis					1376:1383	western blotting analysis	1359:1383	western blotting analysis of ALP	1359:1390	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	44	theme	ALP	1388:1390	arg1	analysis					1194:1201	The host markers analysis	1177:1201	The host markers analysis through protein expression of serum and cecal tissues	1177:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	44	theme	ALP	1388:1390	arg1	PCR					1410:1412	quantitative PCR	1397:1412	quantitative PCR of cecal tissues	1397:1429	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	5	45	theme	sRNA	1132:1135	arg1	sequencing					1137:1146	16 sRNA sequencing	1129:1146	16 sRNA sequencing	1129:1146	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	7	46	theme	system	1728:1733	arg1	ability					1709:1715	the antimicrobial, antioxidant, and anti-inflammatory ability	1655:1715	the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system	1655:1733	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	47	theme	microbial	1581:1589	arg1	richness					1591:1598	commensal microbial richness	1571:1598	commensal microbial richness	1571:1598	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	2	48	theme	reduced	572:578	arg1	production					618:627	reduced intestinal alkaline phosphatase (ALP) production	572:627	reduced intestinal alkaline phosphatase (ALP) production	572:627	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	9	49	theme	pathway-induced	2108:2122	arg1	permeability					2128:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	0	50	theme	intestinal	135:144	arg1	phosphatase					155:165	intestinal alkaline phosphatase	135:165	intestinal alkaline phosphatase enzyme in meat geese	135:186	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	8	51	theme	AGF	1913:1915	arg1	geese					1922:1926	AGF meat geese	1913:1926	AGF meat geese	1913:1926	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	0	52	theme	Pasture	0:6	arg1	intake					8:13	Pasture intake	0:13	Pasture intake	0:13	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	1	53	theme	systemic	320:327	arg1	inflammation					329:340	systemic inflammation	320:340	systemic inflammation	320:340	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	2	54	theme	inflammatory	482:493	arg1	responses					495:503	inflammatory responses	482:503	inflammatory responses	482:503	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	9	55	theme	systemic	2142:2149	arg1	inflammation					2151:2162	systemic inflammation	2142:2162	systemic inflammation	2142:2162	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	2	56	theme	increased	523:531	arg1	lipopolysaccharides					546:564	lipopolysaccharides	546:564	lipopolysaccharides	546:564	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	2	56	theme	increased	523:531	arg1	endotoxemia					533:543	increased endotoxemia	523:543	increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production	523:627	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	10	57	theme	intestinal	2206:2215	arg1	functions					2221:2229	the intestinal ALP functions	2202:2229	the intestinal ALP functions to regulate gut microbial homeostasis and barrier function	2202:2288	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	3	58	theme	fiber	700:704	arg1	AGF					680:682	AGF	680:682	AGF	680:682	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	3	58	theme	fiber	700:704	arg1	source					706:711	a high dietary fiber source	685:711	a high dietary fiber source	685:711	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	2	59	theme	cecal	449:453	arg1	composition					466:476	altered cecal microbiota composition	441:476	altered cecal microbiota composition	441:476	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	0	60	theme	commercial	32:41	arg1	production					75:84	commercial diet-induced lipopolysaccharide production	32:84	commercial diet-induced lipopolysaccharide production facilitated by gut microbiota	32:114	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	3	61	theme	high	687:690	arg1	AGF					680:682	AGF	680:682	AGF	680:682	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	3	61	theme	high	687:690	arg1	source					706:711	a high dietary fiber source	685:711	a high dietary fiber source	685:711	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	4	62	theme	intestinal	875:884	arg1	ALP					886:888	intestinal ALP	875:888	intestinal ALP	875:888	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	6	63	theme	cecal	1417:1421	arg1	tissues					1423:1429	cecal tissues	1417:1429	cecal tissues	1417:1429	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	11	64	theme	novel	2424:2428	arg1	therapy					2430:2436	a novel therapy	2422:2436	a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals	2422:2538	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	2	65	theme	fiber	417:421	arg1	IHF					398:400	IHF	398:400	IHF	398:400	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	2	65	theme	fiber	417:421	arg1	source					423:428	a low dietary fiber source	403:428	a low dietary fiber source	403:428	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	4	66	from	inflammation	1025:1036	arg1	geese					1046:1050	meat geese	1041:1050	meat geese	1041:1050	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	7	67	theme	meat	1501:1504	arg1	analysis					1491:1498	the gut microbiota analysis	1472:1498	the gut microbiota analysis	1472:1498	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	67	theme	meat	1501:1504	arg1	geese					1506:1510	meat geese	1501:1510	meat geese supplemented with pasture	1501:1536	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	9	68	theme	correlation	1942:1952	arg1	analysis					1954:1961	the correlation analysis	1938:1961	the correlation analysis of top 44 host markers with gut microbiota	1938:2004	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	6	69	theme	NF-қB	1312:1316	arg1	staining					1286:1293	hematoxylin and eosin (H&E) staining	1258:1293	hematoxylin and eosin (H&E) staining	1258:1293	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	69	theme	NF-қB	1312:1316	arg1	localization					1296:1307	localization	1296:1307	localization of NF-қB and Nrf2 by immunofluorescence analysis	1296:1356	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	69	theme	NF-қB	1312:1316	arg1	analysis					1376:1383	western blotting analysis	1359:1383	western blotting analysis of ALP	1359:1390	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	69	theme	NF-қB	1312:1316	arg1	analysis					1194:1201	The host markers analysis	1177:1201	The host markers analysis through protein expression of serum and cecal tissues	1177:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	69	theme	NF-қB	1312:1316	arg1	PCR					1410:1412	quantitative PCR	1397:1412	quantitative PCR of cecal tissues	1397:1429	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	2	70	theme	low	405:407	arg1	IHF					398:400	IHF	398:400	IHF	398:400	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	2	70	theme	low	405:407	arg1	source					423:428	a low dietary fiber source	403:428	a low dietary fiber source	403:428	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	1	71	theme	intestinal	287:296	arg1	functions					306:314	the intestinal barrier functions	283:314	the intestinal barrier functions	283:314	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	4	72	theme	critical	903:910	arg1	role					912:915	a critical role	901:915	a critical role	901:915	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	9	73	theme	aging	2169:2173	arg1	phenotypes					2175:2184	aging phenotypes	2169:2184	aging phenotypes	2169:2184	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	3	74	theme	microbiota	732:741	arg1	architecture					743:754	gut microbiota architecture	728:754	gut microbiota architecture	728:754	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	7	75	theme	gut	1476:1478	arg1	analysis					1491:1498	the gut microbiota analysis	1472:1498	the gut microbiota analysis	1472:1498	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	75	theme	gut	1476:1478	arg1	geese					1506:1510	meat geese	1501:1510	meat geese supplemented with pasture	1501:1536	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	10	76	theme	AGF	2377:2379	arg1	geese					2386:2390	AGF meat geese	2377:2390	AGF meat geese	2377:2390	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	10	77	theme	ALP	2217:2219	arg1	functions					2221:2229	the intestinal ALP functions	2202:2229	the intestinal ALP functions to regulate gut microbial homeostasis and barrier function	2202:2288	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	0	78	theme	lipopolysaccharide	56:73	arg1	production					75:84	commercial diet-induced lipopolysaccharide production	32:84	commercial diet-induced lipopolysaccharide production facilitated by gut microbiota	32:114	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	9	79	theme	markers	1978:1984	arg1	analysis					1954:1961	the correlation analysis	1938:1961	the correlation analysis of top 44 host markers with gut microbiota	1938:2004	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	3	80	theme	barrier	764:770	arg1	functions					772:780	gut barrier functions	760:780	gut barrier functions	760:780	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	2	81	theme	feeding	382:388	arg1	system					390:395	In-house feeding system	373:395	In-house feeding system (IHF, a low dietary fiber source)	373:429	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	1	82	theme	gut	224:226	arg1	composition					239:249	gut microbiota composition	224:249	gut microbiota composition	224:249	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	7	83	theme	antioxidant	1674:1684	arg1	ability					1709:1715	the antimicrobial, antioxidant, and anti-inflammatory ability	1655:1715	the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system	1655:1733	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	6	84	theme	markers	1186:1192	arg1	analysis					1194:1201	The host markers analysis	1177:1201	The host markers analysis through protein expression of serum and cecal tissues	1177:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	9	85	theme	gut	1991:1993	arg1	microbiota					1995:2004	gut microbiota	1991:2004	gut microbiota	1991:2004	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	6	86	theme	blotting	1367:1374	arg1	analysis					1376:1383	western blotting analysis	1359:1383	western blotting analysis of ALP	1359:1390	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	10	87	theme	pro-inflammatory	2308:2323	arg1	cytokines					2325:2333	pro-inflammatory cytokines	2308:2333	pro-inflammatory cytokines	2308:2333	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	8	88	theme	intestinal	1762:1771	arg1	pathway					1800:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	3	89	dep	system	672:677	arg1	AGF					680:682	AGF	680:682	AGF	680:682	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	3	89	dep	system	672:677	arg1	source					706:711	a high dietary fiber source	685:711	a high dietary fiber source	685:711	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	7	90	theme	antimicrobial	1659:1671	arg1	ability					1709:1715	the antimicrobial, antioxidant, and anti-inflammatory ability	1655:1715	the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system	1655:1733	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	10	91	theme	gut	2243:2245	arg1	homeostasis					2257:2267	gut microbial homeostasis	2243:2267	gut microbial homeostasis	2243:2267	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	0	92	theme	meat	177:180	arg1	geese					182:186	meat geese	177:186	meat geese	177:186	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	3	93	theme	pasture	656:662	arg1	system					672:677	artificial pasture grazing system	645:677	artificial pasture grazing system (AGF, a high dietary fiber source)	645:712	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	8	94	theme	Nrf2	1785:1788	arg1	pathway					1800:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	2	95	theme	phosphatase	600:610	arg1	production					618:627	reduced intestinal alkaline phosphatase (ALP) production	572:627	reduced intestinal alkaline phosphatase (ALP) production	572:627	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	9	96	theme	artificial	2018:2027	arg1	intake					2037:2042	artificial pasture intake	2018:2042	artificial pasture intake	2018:2042	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	6	97	theme	immunofluorescence	1330:1347	arg1	analysis					1349:1356	immunofluorescence analysis	1330:1356	immunofluorescence analysis	1330:1356	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	7	98	theme	meat	1630:1633	arg1	geese					1635:1639	IHF meat geese	1626:1639	IHF meat geese	1626:1639	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	4	99	theme	reactive	932:939	arg1	species					948:954	reactive oxygen species	932:954	reactive oxygen species (ROS) generation	932:971	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	4	99	theme	reactive	932:939	arg1	ROS					957:959	ROS	957:959	ROS	957:959	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	6	100	theme	Nrf2	1322:1325	arg1	staining					1286:1293	hematoxylin and eosin (H&E) staining	1258:1293	hematoxylin and eosin (H&E) staining	1258:1293	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	100	theme	Nrf2	1322:1325	arg1	localization					1296:1307	localization	1296:1307	localization of NF-қB and Nrf2 by immunofluorescence analysis	1296:1356	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	100	theme	Nrf2	1322:1325	arg1	analysis					1376:1383	western blotting analysis	1359:1383	western blotting analysis of ALP	1359:1390	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	100	theme	Nrf2	1322:1325	arg1	analysis					1194:1201	The host markers analysis	1177:1201	The host markers analysis through protein expression of serum and cecal tissues	1177:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	100	theme	Nrf2	1322:1325	arg1	PCR					1410:1412	quantitative PCR	1397:1412	quantitative PCR of cecal tissues	1397:1429	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	8	101	theme	induced	1877:1883	arg1	mechanisms					1899:1908	TLR4/MyD88 induced ROS reduction mechanisms	1866:1908	TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese	1866:1926	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	4	102	theme	species	948:954	arg1	generation					962:971	reactive oxygen species (ROS) generation	932:971	reactive oxygen species (ROS) generation	932:971	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	6	103	theme	cecal	1243:1247	arg1	tissues					1249:1255	cecal tissues	1243:1255	cecal tissues	1243:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	9	104	theme	gut	2054:2056	arg1	functions					2066:2074	gut barrier functions	2054:2074	gut barrier functions	2054:2074	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	5	105	theme	gut	1099:1101	arg1	composition					1113:1123	gut microbial composition	1099:1123	gut microbial composition via 16 sRNA sequencing	1099:1146	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	3	106	theme	system	672:677	arg1	effects					634:640	The effects	630:640	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions	630:780	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	8	107	theme	LPS	1835:1837	arg1	dephosphorylation					1839:1855	LPS dephosphorylation	1835:1855	LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese	1835:1926	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	4	108	theme	systemic	1016:1023	arg1	inflammation					1025:1036	NF-κB pathway-induced systemic inflammation	994:1036	NF-κB pathway-induced systemic inflammation in meat geese	994:1050	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	6	109	theme	serum	1233:1237	arg1	expression					1219:1228	protein expression	1211:1228	protein expression of serum and cecal tissues	1211:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	110	theme	quantitative	1397:1408	arg1	PCR					1410:1412	quantitative PCR	1397:1412	quantitative PCR of cecal tissues	1397:1429	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	5	111	theme	meat	1165:1168	arg1	geese					1170:1174	meat geese	1165:1174	meat geese	1165:1174	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	1	112	theme	Introduction	189:200	arg1	Diet					202:205	Introduction Diet	189:205	Introduction Diet	189:205	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	7	113	from	Results	1446:1452	arg1	analysis					1491:1498	the gut microbiota analysis	1472:1498	the gut microbiota analysis	1472:1498	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	113	from	Results	1446:1452	arg1	geese					1506:1510	meat geese	1501:1510	meat geese supplemented with pasture	1501:1536	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	0	114	theme	phosphatase	155:165	arg1	enzyme					167:172	intestinal alkaline phosphatase enzyme	135:172	intestinal alkaline phosphatase enzyme in meat geese	135:186	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	2	115	with	endotoxemia	533:543	arg1	production					618:627	reduced intestinal alkaline phosphatase (ALP) production	572:627	reduced intestinal alkaline phosphatase (ALP) production	572:627	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	11	116	theme	inflammatory	2464:2475	arg1	state					2477:2481	the chronic inflammatory state	2452:2481	the chronic inflammatory state leading to low dietary fiber-related diseases in animals	2452:2538	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	7	117	from	Discussion	1458:1467	arg1	analysis					1491:1498	the gut microbiota analysis	1472:1498	the gut microbiota analysis	1472:1498	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	117	from	Discussion	1458:1467	arg1	geese					1506:1510	meat geese	1501:1510	meat geese supplemented with pasture	1501:1536	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	118	theme	commensal	1571:1579	arg1	richness					1591:1598	commensal microbial richness	1571:1598	commensal microbial richness	1571:1598	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	2	119	theme	intestinal	580:589	arg1	ALP					613:615	ALP	613:615	ALP	613:615	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	2	119	theme	intestinal	580:589	arg1	phosphatase					600:610	intestinal alkaline phosphatase	580:610	reduced intestinal alkaline phosphatase (ALP) production	572:627	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	9	120	theme	NF-κB	2102:2106	arg1	permeability					2128:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	8	121	theme	signaling	1790:1798	arg1	pathway					1800:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	0	122	theme	alkaline	146:153	arg1	phosphatase					155:165	intestinal alkaline phosphatase	135:165	intestinal alkaline phosphatase enzyme in meat geese	135:186	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	8	123	theme	reduction	1889:1897	arg1	mechanisms					1899:1908	TLR4/MyD88 induced ROS reduction mechanisms	1866:1908	TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese	1866:1926	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	4	124	theme	NF-κB	994:998	arg1	inflammation					1025:1036	NF-κB pathway-induced systemic inflammation	994:1036	NF-κB pathway-induced systemic inflammation in meat geese	994:1050	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	9	125	theme	gut	2124:2126	arg1	permeability					2128:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	ROS-mediated NF-κB pathway-induced gut permeability	2089:2139	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	4	126	theme	pathway-induced	1000:1014	arg1	inflammation					1025:1036	NF-κB pathway-induced systemic inflammation	994:1036	NF-κB pathway-induced systemic inflammation in meat geese	994:1050	Therefore, this study aimed to investigate whether intestinal ALP could play a critical role in attenuating reactive oxygen species (ROS) generation and ROS facilitating NF-κB pathway-induced systemic inflammation in meat geese.
36569878	7	127	theme	AGF	1724:1726	arg1	system					1728:1733	the AGF system	1720:1733	the AGF system	1720:1733	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	10	128	theme	ROS	2359:2361	arg1	production					2363:2372	LPS-induced ROS production	2347:2372	LPS-induced ROS production	2347:2372	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	2	129	dep	system	390:395	arg1	IHF					398:400	IHF	398:400	IHF	398:400	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	2	129	dep	system	390:395	arg1	source					423:428	a low dietary fiber source	403:428	a low dietary fiber source	403:428	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	8	130	theme	meat	1917:1920	arg1	geese					1922:1926	AGF meat geese	1913:1926	AGF meat geese	1913:1926	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	3	131	theme	dietary	692:698	arg1	AGF					680:682	AGF	680:682	AGF	680:682	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	3	131	theme	dietary	692:698	arg1	source					706:711	a high dietary fiber source	685:711	a high dietary fiber source	685:711	The effects of artificial pasture grazing system (AGF, a high dietary fiber source) on modulating gut microbiota architecture and gut barrier functions have not been investigated in meat geese.
36569878	2	132	theme	microbiota	455:464	arg1	composition					466:476	altered cecal microbiota composition	441:476	altered cecal microbiota composition	441:476	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	0	133	theme	diet-induced	43:54	arg1	production					75:84	commercial diet-induced lipopolysaccharide production	32:84	commercial diet-induced lipopolysaccharide production facilitated by gut microbiota	32:114	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	1	134	theme	metabolic	350:358	arg1	endotoxemia					360:370	metabolic endotoxemia	350:370	metabolic endotoxemia	350:370	Introduction Diet strongly affects gut microbiota composition, and gut bacteria can influence the intestinal barrier functions and systemic inflammation through metabolic endotoxemia.
36569878	7	135	theme	anti-inflammatory	1691:1707	arg1	ability					1709:1715	the antimicrobial, antioxidant, and anti-inflammatory ability	1655:1715	the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system	1655:1733	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	8	136	from	increase	1750:1757	arg1	pathway					1800:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	intestinal ALP-induced Nrf2 signaling pathway	1762:1806	A significant increase in intestinal ALP-induced Nrf2 signaling pathway was confirmed representing LPS dephosphorylation mediated TLR4/MyD88 induced ROS reduction mechanisms in AGF meat geese.
36569878	2	137	theme	altered	441:447	arg1	composition					466:476	altered cecal microbiota composition	441:476	altered cecal microbiota composition	441:476	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	6	138	theme	tissues	1423:1429	arg1	staining					1286:1293	hematoxylin and eosin (H&E) staining	1258:1293	hematoxylin and eosin (H&E) staining	1258:1293	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	138	theme	tissues	1423:1429	arg1	localization					1296:1307	localization	1296:1307	localization of NF-қB and Nrf2 by immunofluorescence analysis	1296:1356	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	138	theme	tissues	1423:1429	arg1	analysis					1376:1383	western blotting analysis	1359:1383	western blotting analysis of ALP	1359:1390	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	138	theme	tissues	1423:1429	arg1	analysis					1194:1201	The host markers analysis	1177:1201	The host markers analysis through protein expression of serum and cecal tissues	1177:1255	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	6	138	theme	tissues	1423:1429	arg1	PCR					1410:1412	quantitative PCR	1397:1412	quantitative PCR of cecal tissues	1397:1429	The host markers analysis through protein expression of serum and cecal tissues, hematoxylin and eosin (H&E) staining, localization of NF-қB and Nrf2 by immunofluorescence analysis, western blotting analysis of ALP, and quantitative PCR of cecal tissues was evaluated.
36569878	7	139	from	diversity	1604:1612	arg1	richness					1591:1598	commensal microbial richness	1571:1598	commensal microbial richness	1571:1598	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	7	140	theme	significant	1547:1557	arg1	increase					1559:1566	a significant increase	1545:1566	a significant increase in commensal microbial richness	1545:1598	Results and Discussion In the gut microbiota analysis, meat geese supplemented with pasture showed a significant increase in commensal microbial richness and diversity compared to IHF meat geese demonstrating the antimicrobial, antioxidant, and anti-inflammatory ability of the AGF system.
36569878	5	141	theme	IHF	1076:1078	arg1	impacts					1065:1071	The impacts	1061:1071	The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing	1061:1146	Methods The impacts of IHF and AGF systems on gut microbial composition via 16 sRNA sequencing were assessed in meat geese.
36569878	10	142	dep	functions	2221:2229	arg1	regulate					2234:2241	regulate	2234:2241	to regulate gut microbial homeostasis and barrier function	2231:2288	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	2	143	theme	meat	508:511	arg1	geese					513:517	meat geese	508:517	meat geese	508:517	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	9	144	with	markers	1978:1984	arg1	microbiota					1995:2004	gut microbiota	1991:2004	gut microbiota	1991:2004	Further, the correlation analysis of top 44 host markers with gut microbiota showed that artificial pasture intake protected gut barrier functions via reducing ROS-mediated NF-κB pathway-induced gut permeability, systemic inflammation, and aging phenotypes.
36569878	11	145	theme	fiber-related	2506:2518	arg1	diseases					2520:2527	low dietary fiber-related diseases	2494:2527	low dietary fiber-related diseases in animals	2494:2538	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	10	146	theme	barrier	2273:2279	arg1	function					2281:2288	barrier function	2273:2288	barrier function	2273:2288	In conclusion, the intestinal ALP functions to regulate gut microbial homeostasis and barrier function appear to inhibit pro-inflammatory cytokines by reducing LPS-induced ROS production in AGF meat geese.
36569878	11	147	from	diseases	2520:2527	arg1	animals					2532:2538	animals	2532:2538	animals	2532:2538	The AGF system may represent a novel therapy to counteract the chronic inflammatory state leading to low dietary fiber-related diseases in animals.
36569878	0	148	from	enzyme	167:172	arg1	geese					182:186	meat geese	177:186	meat geese	177:186	Pasture intake protects against commercial diet-induced lipopolysaccharide production facilitated by gut microbiota through activating intestinal alkaline phosphatase enzyme in meat geese.
36569878	2	149	theme	dietary	409:415	arg1	IHF					398:400	IHF	398:400	IHF	398:400	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
36569878	2	149	theme	dietary	409:415	arg1	source					423:428	a low dietary fiber source	403:428	a low dietary fiber source	403:428	In-house feeding system (IHF, a low dietary fiber source) may cause altered cecal microbiota composition and inflammatory responses in meat geese via increased endotoxemia (lipopolysaccharides) with reduced intestinal alkaline phosphatase (ALP) production.
37155011	4	0	theme	specific	661:668	arg1	markers					682:688	13 cellulose synthase specific polymorphic markers	639:688	13 cellulose synthase specific polymorphic markers	639:688	For genotyping, 13 cellulose synthase specific polymorphic markers were used.
37155011	11	1	theme	moderate	1398:1405	arg1	level					1407:1411	The moderate level	1394:1411	CONCLUSIONS The moderate level of both PIC and H average estimates	1382:1447	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	13	2	theme	cellulose	1972:1980	arg1	accumulation					1982:1993	higher cellulose accumulation	1965:1993	higher cellulose accumulation	1965:1993	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	8	3	theme	genotypes	1183:1191	arg1	genotypes					1183:1191	genotypes	1183:1191	genotypes	1183:1191	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	8	3	theme	genotypes	1183:1191	arg1	system					1204:1209	marker system	1197:1209	marker system	1197:1209	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	8	3	theme	genotypes	1183:1191	arg1	set					1176:1178	current set	1168:1178	current set of genotypes	1168:1191	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	2	4	theme	calibre	313:319	arg1	calibre					313:319	such calibre	308:319	such calibre	308:319	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	2	4	theme	calibre	313:319	arg1	varieties					295:303	rice varieties	290:303	rice varieties of such calibre	290:319	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	8	5	theme	marker	1197:1202	arg1	system					1204:1209	marker system	1197:1209	marker system	1197:1209	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	11	6	theme	PIC	1421:1423	arg1	level					1407:1411	The moderate level	1394:1411	CONCLUSIONS The moderate level of both PIC and H average estimates	1382:1447	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	4	7	theme	cellulose	642:650	arg1	markers					682:688	13 cellulose synthase specific polymorphic markers	639:688	13 cellulose synthase specific polymorphic markers	639:688	For genotyping, 13 cellulose synthase specific polymorphic markers were used.
37155011	14	8	theme	food	2116:2119	arg1	security					2121:2128	food security	2116:2128	food security	2116:2128	This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
37155011	2	9	theme	biochemical	334:344	arg1	characterization					346:361	biochemical characterization	334:361	biochemical characterization	334:361	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	12	10	theme	bioenergy	1650:1658	arg1	varieties					1670:1678	bioenergy efficient varieties	1650:1678	bioenergy efficient varieties	1650:1678	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	4	11	used	used	695:698	arg2	markers					682:688	13 cellulose synthase specific polymorphic markers	639:688	13 cellulose synthase specific polymorphic markers	639:688	For genotyping, 13 cellulose synthase specific polymorphic markers were used.
37155011	7	12	theme	OsCESA-6.3	1072:1081	arg1	0.128					1098:1102	0.128	1098:1102	0.128	1098:1102	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	7	12	theme	OsCESA-6.3	1072:1081	arg1	PIC					1093:1095	the marker OsCESA-6.3 of lowest PIC	1061:1095	the marker OsCESA-6.3 of lowest PIC (0.128)	1061:1103	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	1	13	theme	human	242:246	arg1	dependence					228:237	the dependence	224:237	the dependence of human on non-renewable fuel sources	224:276	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	8	14	dep	average	1117:1123	arg1	estimate					1125:1132	estimate	1125:1132	estimate	1125:1132	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	5	15	theme	×	766:766	arg1	6.51b2					768:773	GenAlE × 6.51b2	759:773	GenAlE × 6.51b2	759:773	The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program.
37155011	5	15	theme	×	766:766	arg1	program					785:791	software program	776:791	software program	776:791	The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program.
37155011	12	16	theme	hybridization	1615:1627	arg1	programme					1629:1637	a hybridization programme	1613:1637	a hybridization programme	1613:1637	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	7	17	theme	marker	1065:1070	arg1	0.128					1098:1102	0.128	1098:1102	0.128	1098:1102	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	7	17	theme	marker	1065:1070	arg1	PIC					1093:1095	the marker OsCESA-6.3 of lowest PIC	1061:1095	the marker OsCESA-6.3 of lowest PIC (0.128)	1061:1103	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	2	18	theme	rice	290:293	arg1	calibre					313:319	such calibre	308:319	such calibre	308:319	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	2	18	theme	rice	290:293	arg1	varieties					295:303	rice varieties	290:303	rice varieties of such calibre	290:319	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	11	19	theme	germplasms	1490:1499	arg1	bases					1477:1481	the narrow genetic bases	1458:1481	the narrow genetic bases of the germplasms	1458:1499	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	0	20	theme	rice	62:65	arg1	varieties					67:75	elite rice varieties	56:75	elite rice varieties for straw lignocellulose	56:100	Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose.
37155011	1	21	theme	enormous	164:171	arg1	potential					173:181	enormous potential	164:181	enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources	164:276	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	12	22	used	used	1605:1608	arg2	varieties					1506:1514	The varieties	1502:1514	The varieties falling under different clusters possessing desirable lignocellulosic composition	1502:1596	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	13	23	theme	bioenergy	1760:1768	arg1	genotypes					1780:1788	bioenergy efficient genotypes	1760:1788	bioenergy efficient genotypes	1760:1788	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	14	24	theme	dual	2044:2047	arg1	varieties					2062:2070	suitable dual purpose rice varieties	2035:2070	suitable dual purpose rice varieties for biofuel production	2035:2093	This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
37155011	12	25	theme	desirable	1560:1568	arg1	composition					1586:1596	desirable lignocellulosic composition	1560:1596	desirable lignocellulosic composition	1560:1596	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	2	26	theme	rice	428:431	arg1	genotypes					433:441	the rice genotypes	424:441	the rice genotypes with respect to cellulose content	424:475	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	13	27	theme	varietal	1695:1702	arg1	combinations					1704:1715	The promising varietal combinations	1681:1715	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.	1681:1994	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	27	theme	varietal	1695:1702	arg1	parents					1737:1743	parents	1737:1743	parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation	1737:1993	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	9	28	theme	dendrogram	1216:1225	arg1	analysis					1227:1234	The dendrogram analysis	1212:1234	The dendrogram analysis	1212:1234	The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II.
37155011	3	29	theme	rice	516:519	arg1	genotypes					521:529	Forty-three elite rice genotypes	498:529	METHODS AND RESULTS Forty-three elite rice genotypes	478:529	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37155011	3	30	theme	genetic	599:605	arg1	fingerprinting					607:620	SSR marker-based genetic fingerprinting	582:620	SSR marker-based genetic fingerprinting	582:620	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37155011	6	31	theme	lignocellulosic	930:944	arg1	composition					946:956	desirable lignocellulosic composition	920:956	desirable lignocellulosic composition	920:956	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	1	32	theme	Lignocellulosic	114:128	arg1	biomass					130:136	BACKGROUND Lignocellulosic biomass	103:136	BACKGROUND Lignocellulosic biomass from rice straw	103:152	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	11	33	theme	genetic	1469:1475	arg1	bases					1477:1481	the narrow genetic bases	1458:1481	the narrow genetic bases of the germplasms	1458:1499	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	3	34	theme	Forty-three	498:508	arg1	genotypes					521:529	Forty-three elite rice genotypes	498:529	METHODS AND RESULTS Forty-three elite rice genotypes	478:529	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37155011	0	35	theme	molecular	26:34	arg1	characterization					36:51	molecular characterization	26:51	molecular characterization of elite rice varieties for straw lignocellulose	26:100	Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose.
37155011	12	36	theme	different	1530:1538	arg1	clusters					1540:1547	different clusters	1530:1547	different clusters possessing desirable lignocellulosic composition	1530:1596	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	3	37	dep	METHODS	478:484	arg1	genotypes					521:529	Forty-three elite rice genotypes	498:529	METHODS AND RESULTS Forty-three elite rice genotypes	478:529	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37155011	14	38	theme	biofuel	2076:2082	arg1	production					2084:2093	biofuel production	2076:2093	biofuel production	2076:2093	This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
37155011	14	39	theme	varieties	2062:2070	arg1	identification					2017:2030	identification	2017:2030	identification of suitable dual purpose rice varieties for biofuel production	2017:2093	This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
37155011	8	40	theme	PIC	1145:1147	arg1	average					1117:1123	average	1117:1123	average	1117:1123	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	8	40	theme	PIC	1145:1147	arg1	0.367					1135:1139	0.367	1135:1139	0.367	1135:1139	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	6	41	theme	rice	804:807	arg1	varieties					809:817	the 43 rice varieties	797:817	the 43 rice varieties	797:817	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	3	42	theme	SSR	582:584	arg1	fingerprinting					607:620	SSR marker-based genetic fingerprinting	582:620	SSR marker-based genetic fingerprinting	582:620	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37155011	1	43	theme	fuel	265:268	arg1	sources					270:276	non-renewable fuel sources	251:276	non-renewable fuel sources	251:276	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	2	44	theme	genetic	400:406	arg1	diversity					408:416	genetic diversity	400:416	genetic diversity among the rice genotypes with respect to cellulose content	400:475	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	8	45	theme	current	1168:1174	arg1	genotypes					1183:1191	genotypes	1183:1191	genotypes	1183:1191	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	8	45	theme	current	1168:1174	arg1	set					1176:1178	current set	1168:1178	current set of genotypes	1168:1191	A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system.
37155011	6	46	theme	green	985:989	arg1	fuels					991:995	green fuels	985:995	green fuels	985:995	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	4	47	theme	synthase	652:659	arg1	markers					682:688	13 cellulose synthase specific polymorphic markers	639:688	13 cellulose synthase specific polymorphic markers	639:688	For genotyping, 13 cellulose synthase specific polymorphic markers were used.
37155011	13	48	theme	accumulation	1982:1993	arg1	advantage					1952:1960	an advantage	1949:1960	an advantage of higher cellulose accumulation	1949:1993	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	4	49	theme	polymorphic	670:680	arg1	markers					682:688	13 cellulose synthase specific polymorphic markers	639:688	13 cellulose synthase specific polymorphic markers	639:688	For genotyping, 13 cellulose synthase specific polymorphic markers were used.
37155011	5	50	theme	diversity	705:713	arg1	analysis					715:722	The diversity analysis	701:722	The diversity analysis	701:722	The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program.
37155011	12	51	contain	possessing	1549:1558	arg1	clusters					1540:1547	different clusters	1530:1547	different clusters possessing desirable lignocellulosic composition	1530:1596	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	12	51	contain	possessing	1549:1558	arg2	composition					1586:1596	desirable lignocellulosic composition	1560:1596	desirable lignocellulosic composition	1560:1596	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	2	52	theme	such	308:311	arg1	calibre					313:319	such calibre	308:319	such calibre	308:319	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	1	53	contain	possesses	154:162	arg1	biomass					130:136	BACKGROUND Lignocellulosic biomass	103:136	BACKGROUND Lignocellulosic biomass from rice straw	103:152	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	1	53	contain	possesses	154:162	arg2	potential					173:181	enormous potential	164:181	enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources	164:276	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	11	54	dep	CONCLUSIONS	1382:1392	arg1	level					1407:1411	The moderate level	1394:1411	CONCLUSIONS The moderate level of both PIC and H average estimates	1382:1447	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	5	55	theme	software	776:783	arg1	6.51b2					768:773	GenAlE × 6.51b2	759:773	GenAlE × 6.51b2	759:773	The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program.
37155011	5	55	theme	software	776:783	arg1	program					785:791	software program	776:791	software program	776:791	The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program.
37155011	13	56	theme	higher	1965:1970	arg1	accumulation					1982:1993	higher cellulose accumulation	1965:1993	higher cellulose accumulation	1965:1993	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	12	57	theme	efficient	1660:1668	arg1	varieties					1670:1678	bioenergy efficient varieties	1650:1678	bioenergy efficient varieties	1650:1678	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	5	58	theme	GenAlE	759:764	arg1	6.51b2					768:773	GenAlE × 6.51b2	759:773	GenAlE × 6.51b2	759:773	The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program.
37155011	5	58	theme	GenAlE	759:764	arg1	program					785:791	software program	776:791	software program	776:791	The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program.
37155011	9	59	theme	principal	1272:1280	arg1	clusters					1282:1289	two principal clusters	1268:1289	two principal clusters i.e., cluster I and II	1268:1312	The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II.
37155011	7	60	theme	of	1083:1084	arg1	0.128					1098:1102	0.128	1098:1102	0.128	1098:1102	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	7	60	theme	of	1083:1084	arg1	PIC					1093:1095	the marker OsCESA-6.3 of lowest PIC	1061:1095	the marker OsCESA-6.3 of lowest PIC (0.128)	1061:1103	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	11	61	dep	PIC	1421:1423	arg1	estimates					1439:1447	estimates	1439:1447	estimates	1439:1447	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	6	62	contain	possess	912:918	arg2	composition					946:956	desirable lignocellulosic composition	920:956	desirable lignocellulosic composition	920:956	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	6	62	contain	possess	912:918	arg1	Jagabandhu					857:866	Jagabandhu	857:866	Jagabandhu	857:866	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	6	62	contain	possess	912:918	arg1	Chandrama					888:896	Chandrama	888:896	Chandrama	888:896	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	6	62	contain	possess	912:918	arg1	CR-Dhan-601					820:830	CR-Dhan-601	820:830	CR-Dhan-601	820:830	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	6	62	contain	possess	912:918	arg1	CR-Dhan-1014					833:844	CR-Dhan-1014	833:844	CR-Dhan-1014	833:844	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	6	62	contain	possess	912:918	arg1	Samanta					876:882	Samanta	876:882	Samanta	876:882	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	9	63	theme	rice	1248:1251	arg1	genotypes					1253:1261	the rice genotypes	1244:1261	the rice genotypes	1244:1261	The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II.
37155011	7	64	theme	lowest	1086:1091	arg1	0.128					1098:1102	0.128	1098:1102	0.128	1098:1102	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	7	64	theme	lowest	1086:1091	arg1	PIC					1093:1095	the marker OsCESA-6.3 of lowest PIC	1061:1095	the marker OsCESA-6.3 of lowest PIC (0.128)	1061:1103	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	1	65	from	dependence	228:237	arg1	sources					270:276	non-renewable fuel sources	251:276	non-renewable fuel sources	251:276	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	1	66	theme	rice	143:146	arg1	straw					148:152	rice straw	143:152	rice straw	143:152	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	0	67	theme	varieties	67:75	arg1	characterization					36:51	molecular characterization	26:51	molecular characterization of elite rice varieties for straw lignocellulose	26:100	Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose.
37155011	9	68	theme	cluster	1297:1303	arg1	I					1305:1305	cluster I	1297:1305	cluster I	1297:1305	The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II.
37155011	0	69	theme	straw	81:85	arg1	lignocellulose					87:100	straw lignocellulose	81:100	straw lignocellulose	81:100	Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose.
37155011	14	70	theme	rice	2057:2060	arg1	varieties					2062:2070	suitable dual purpose rice varieties	2035:2070	suitable dual purpose rice varieties for biofuel production	2035:2093	This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
37155011	13	71	used	used	1729:1732	arg2	combinations					1704:1715	The promising varietal combinations	1681:1715	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.	1681:1994	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	71	used	used	1729:1732	arg2	parents					1737:1743	parents	1737:1743	parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation	1737:1993	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	14	72	theme	purpose	2049:2055	arg1	varieties					2062:2070	suitable dual purpose rice varieties	2035:2070	suitable dual purpose rice varieties for biofuel production	2035:2093	This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
37155011	12	73	theme	lignocellulosic	1570:1584	arg1	composition					1586:1596	desirable lignocellulosic composition	1560:1596	desirable lignocellulosic composition	1560:1596	The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties.
37155011	13	74	theme	efficient	1770:1778	arg1	genotypes					1780:1788	bioenergy efficient genotypes	1760:1788	bioenergy efficient genotypes	1760:1788	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	Mahanadi					1836:1843	Mahanadi	1836:1843	Mahanadi	1836:1843	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	Rambha					1874:1879	Rambha	1874:1879	Rambha	1874:1879	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	Indravati					1900:1908	Indravati	1900:1908	Indravati	1900:1908	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	Mahanadi					1813:1820	Mahanadi	1813:1820	Mahanadi	1813:1820	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	CR-Dhan-601					1914:1924	CR-Dhan-601	1914:1924	CR-Dhan-601	1914:1924	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	Mahanadi					1855:1862	Mahanadi	1855:1862	Mahanadi	1855:1862	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	Rambha					1891:1896	Rambha	1891:1896	Rambha	1891:1896	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	75	dep	Kanchan	1794:1800	arg1	Manika					1928:1933	Manika	1928:1933	Manika	1928:1933	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	7	76	theme	highest	1034:1040	arg1	0.640					1047:1051	0.640	1047:1051	0.640	1047:1051	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	7	76	theme	highest	1034:1040	arg1	PIC					1042:1044	the highest PIC	1030:1044	the highest PIC (0.640)	1030:1052	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	14	77	theme	suitable	2035:2042	arg1	varieties					2062:2070	suitable dual purpose rice varieties	2035:2070	suitable dual purpose rice varieties for biofuel production	2035:2093	This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
37155011	11	78	theme	average	1431:1437	arg1	level					1407:1411	The moderate level	1394:1411	CONCLUSIONS The moderate level of both PIC and H average estimates	1382:1447	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	1	79	from	straw	148:152	arg1	biomass					130:136	BACKGROUND Lignocellulosic biomass	103:136	BACKGROUND Lignocellulosic biomass from rice straw	103:152	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	7	80	theme	marker	1002:1007	arg1	OsCESA-1.3					1009:1018	The marker OsCESA-1.3	998:1018	The marker OsCESA-1.3	998:1018	The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128).
37155011	1	81	theme	BACKGROUND	103:112	arg1	biomass					130:136	BACKGROUND Lignocellulosic biomass	103:136	BACKGROUND Lignocellulosic biomass from rice straw	103:152	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	9	82	dep	I	1305:1305	arg1	i.e.					1291:1294	i.e.	1291:1294	i.e.	1291:1294	The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II.
37155011	11	83	theme	narrow	1462:1467	arg1	bases					1477:1481	the narrow genetic bases	1458:1481	the narrow genetic bases of the germplasms	1458:1499	CONCLUSIONS The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms.
37155011	2	84	theme	cellulose	459:467	arg1	content					469:475	cellulose content	459:475	cellulose content	459:475	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	6	85	theme	desirable	920:928	arg1	composition					946:956	desirable lignocellulosic composition	920:956	desirable lignocellulosic composition	920:956	Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels.
37155011	3	86	theme	elite	510:514	arg1	genotypes					521:529	Forty-three elite rice genotypes	498:529	METHODS AND RESULTS Forty-three elite rice genotypes	478:529	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37155011	0	87	theme	elite	56:60	arg1	varieties					67:75	elite rice varieties	56:75	elite rice varieties for straw lignocellulose	56:100	Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose.
37155011	3	88	theme	biochemical	549:559	arg1	characterization					561:576	biochemical characterization	549:576	biochemical characterization	549:576	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37155011	13	89	theme	promising	1685:1693	arg1	combinations					1704:1715	The promising varietal combinations	1681:1715	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.	1681:1994	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	13	89	theme	promising	1685:1693	arg1	parents					1737:1743	parents	1737:1743	parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation	1737:1993	The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation.
37155011	2	90	theme	diversity	408:416	arg1	presence					388:395	the presence	384:395	the presence of genetic diversity among the rice genotypes with respect to cellulose content	384:475	Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
37155011	1	91	theme	non-renewable	251:263	arg1	sources					270:276	non-renewable fuel sources	251:276	non-renewable fuel sources	251:276	BACKGROUND Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources.
37155011	3	92	theme	marker-based	586:597	arg1	fingerprinting					607:620	SSR marker-based genetic fingerprinting	582:620	SSR marker-based genetic fingerprinting	582:620	METHODS AND RESULTS Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting.
37178747	8	0	theme	bacterial	1139:1147	arg1	proliferation					1149:1161	bacterial proliferation	1139:1161	bacterial proliferation	1139:1161	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	7	1	theme	polymeric	959:967	arg1	substances					969:978	extracellular polymeric substances	945:978	extracellular polymeric substances	945:978	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	5	2	theme	%	676:676	arg1	%					684:684	60% to >80%	674:684	60% to >80%	674:684	The degradation efficiency of 2-CP was improved from 60% to >80% under LPS treatment.
37178747	7	3	theme	activity	932:939	arg1	level					905:909	the level	901:909	the level of electron transfer activity and extracellular polymeric substances secretion	901:988	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	1	4	dep	Lactobacillus	84:96	arg1	plantarum					98:106	plantarum	98:106	plantarum	98:106	stimulated by Lactobacillus plantarum fermentation waste: A bio-waste reuse.
37178747	3	5	theme	fermentation	371:382	arg1	LPS					400:402	LPS	400:402	LPS	400:402	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	3	5	theme	fermentation	371:382	arg1	solution					390:397	Lactobacillus plantarum fermentation waste solution	347:397	Lactobacillus plantarum fermentation waste solution (LPS)	347:403	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	9	6	theme	fermentation	1296:1307	arg1	streams					1315:1321	fermentation waste streams	1296:1321	fermentation waste streams in biostimulation methods	1296:1347	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	6	7	theme	species	798:804	arg1	level					773:777	the level	769:777	the level of reactive oxygen species	769:804	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	2	8	theme	more	307:310	arg1	attention					321:329	more and more attention	307:329	more and more attention	307:329	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	4	9	theme	physiology	589:598	arg1	terms					570:574	terms	570:574	terms of both cell physiology and transcriptomics	570:618	Strain ZY1 in terms of both cell physiology and transcriptomics.
37178747	3	10	theme	waste	384:388	arg1	LPS					400:402	LPS	400:402	LPS	400:402	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	3	10	theme	waste	384:388	arg1	solution					390:397	Lactobacillus plantarum fermentation waste solution	347:397	Lactobacillus plantarum fermentation waste solution (LPS)	347:403	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	9	11	theme	waste	1309:1313	arg1	streams					1315:1321	fermentation waste streams	1296:1321	fermentation waste streams in biostimulation methods	1296:1347	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	7	12	theme	metabolic	1007:1015	arg1	activity					1017:1024	the metabolic activity	1003:1024	the metabolic activity of the strain	1003:1038	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	6	13	theme	oxygen	791:796	arg1	species					798:804	reactive oxygen species	782:804	reactive oxygen species	782:804	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	4	14	theme	cell	584:587	arg1	physiology					589:598	cell physiology	584:598	cell physiology	584:598	Strain ZY1 in terms of both cell physiology and transcriptomics.
37178747	3	15	theme	Lactobacillus	347:359	arg1	LPS					400:402	LPS	400:402	LPS	400:402	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	3	15	theme	Lactobacillus	347:359	arg1	solution					390:397	Lactobacillus plantarum fermentation waste solution	347:397	Lactobacillus plantarum fermentation waste solution (LPS)	347:403	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	2	16	theme	Green	147:151	arg1	methods					189:195	Green and economical pollution management methods	147:195	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants	147:291	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	5	17	theme	degradation	625:635	arg1	efficiency					637:646	The degradation efficiency	621:646	The degradation efficiency of 2-CP	621:654	The degradation efficiency of 2-CP was improved from 60% to >80% under LPS treatment.
37178747	6	18	theme	reactive	782:789	arg1	species					798:804	reactive oxygen species	782:804	reactive oxygen species	782:804	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	3	19	theme	strain	531:536	arg1	sp					552:553	strain Acinetobacter sp	531:553	strain Acinetobacter sp	531:553	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	3	20	theme	plantarum	361:369	arg1	LPS					400:402	LPS	400:402	LPS	400:402	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	3	20	theme	plantarum	361:369	arg1	solution					390:397	Lactobacillus plantarum fermentation waste solution	347:397	Lactobacillus plantarum fermentation waste solution (LPS)	347:403	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	8	21	theme	energy	1212:1217	arg1	conversion					1219:1228	energy conversion	1212:1228	energy conversion	1212:1228	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	6	22	theme	membrane	830:837	arg1	permeability					839:850	the cell membrane permeability	821:850	the cell membrane permeability	821:850	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	2	23	theme	pollutants	282:291	arg1	removal					264:270	the removal	260:270	the removal of target pollutants	260:291	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	7	24	theme	electron	914:921	arg1	activity					932:939	electron transfer activity	914:939	electron transfer activity	914:939	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	9	25	theme	biostimulation	1326:1339	arg1	methods					1341:1347	biostimulation methods	1326:1347	biostimulation methods	1326:1347	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	9	26	from	methods	1341:1347	arg1	reuse					1287:1291	the reuse	1283:1291	the reuse of fermentation waste streams in biostimulation methods	1283:1347	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	6	27	theme	22	864:865	arg1	%					859:859	%	859:859	%	859:859	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	8	28	theme	membrane	1176:1183	arg1	composition					1195:1205	membrane structure composition	1176:1205	membrane structure composition	1176:1205	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	8	29	theme	LPS	1095:1097	arg1	stimulation					1080:1090	the stimulation	1076:1090	the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion	1076:1228	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	5	30	theme	2-CP	651:654	arg1	efficiency					637:646	The degradation efficiency	621:646	The degradation efficiency of 2-CP	621:654	The degradation efficiency of 2-CP was improved from 60% to >80% under LPS treatment.
37178747	1	31	theme	Lactobacillus	84:96	arg1	waste					121:125	Lactobacillus plantarum fermentation waste	84:125	Lactobacillus plantarum fermentation waste	84:125	stimulated by Lactobacillus plantarum fermentation waste: A bio-waste reuse.
37178747	3	32	theme	facilitative	433:444	arg1	effect					446:451	its facilitative effect	429:451	its facilitative effect	429:451	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	0	33	theme	mechanism	19:27	arg1	Exploration					0:10	Exploration	0:10	Exploration of the mechanism of 2-CP degradation by Acinetobacter sp.	0:68	Exploration of the mechanism of 2-CP degradation by Acinetobacter sp.
37178747	7	34	theme	transfer	923:930	arg1	activity					932:939	electron transfer activity	914:939	electron transfer activity	914:939	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	2	35	theme	more	316:319	arg1	attention					321:329	more and more attention	307:329	more and more attention	307:329	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	9	36	from	streams	1315:1321	arg1	methods					1341:1347	biostimulation methods	1326:1347	biostimulation methods	1326:1347	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	5	37	theme	LPS	692:694	arg1	treatment					696:704	LPS treatment	692:704	LPS treatment	692:704	The degradation efficiency of 2-CP was improved from 60% to >80% under LPS treatment.
37178747	6	38	theme	39	857:858	arg1	%					859:859	%	859:859	%	859:859	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	1	39	theme	fermentation	108:119	arg1	waste					121:125	Lactobacillus plantarum fermentation waste	84:125	Lactobacillus plantarum fermentation waste	84:125	stimulated by Lactobacillus plantarum fermentation waste: A bio-waste reuse.
37178747	0	40	theme	2-CP	32:35	arg1	degradation					37:47	2-CP degradation	32:47	2-CP degradation	32:47	Exploration of the mechanism of 2-CP degradation by Acinetobacter sp.
37178747	3	41	theme	2-chlorophenol	506:519	arg1	degradation					491:501	the degradation	487:501	the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp	487:553	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	9	42	theme	streams	1315:1321	arg1	reuse					1287:1291	the reuse	1283:1291	the reuse of fermentation waste streams in biostimulation methods	1283:1347	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	2	43	theme	management	178:187	arg1	methods					189:195	Green and economical pollution management methods	147:195	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants	147:291	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	2	44	theme	pollution	168:176	arg1	methods					189:195	Green and economical pollution management methods	147:195	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants	147:291	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	3	45	theme	stimulation	461:471	arg1	mechanisms					473:482	the stimulation mechanisms	457:482	the stimulation mechanisms	457:482	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	3	46	used	used	409:412	arg2	solution					390:397	Lactobacillus plantarum fermentation waste solution	347:397	Lactobacillus plantarum fermentation waste solution (LPS)	347:403	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	3	46	used	used	409:412	arg2	LPS					400:402	LPS	400:402	LPS	400:402	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	2	47	theme	economical	157:166	arg1	methods					189:195	Green and economical pollution management methods	147:195	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants	147:291	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	6	48	theme	strain	753:758	arg1	morphology					739:748	the morphology	735:748	the morphology of strain	735:758	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	0	49	theme	degradation	37:47	arg1	mechanism					19:27	the mechanism	15:27	the mechanism of 2-CP degradation	15:47	Exploration of the mechanism of 2-CP degradation by Acinetobacter sp.
37178747	7	50	theme	strain	1033:1038	arg1	activity					1017:1024	the metabolic activity	1003:1024	the metabolic activity of the strain	1003:1038	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	6	51	theme	cell	825:828	arg1	permeability					839:850	the cell membrane permeability	821:850	the cell membrane permeability	821:850	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	9	52	theme	new	1251:1253	arg1	insights					1255:1262	new insights	1251:1262	new insights	1251:1262	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	3	53	from	mechanisms	473:482	arg1	degradation					491:501	the degradation	487:501	the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp	487:553	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	1	54	dep	stimulated	70:79	arg1	reuse					140:144	A bio-waste reuse	128:144	A bio-waste reuse	128:144	stimulated by Lactobacillus plantarum fermentation waste: A bio-waste reuse.
37178747	8	55	theme	biological	1110:1119	arg1	composition					1195:1205	membrane structure composition	1176:1205	membrane structure composition	1176:1205	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	8	55	theme	biological	1110:1119	arg1	conversion					1219:1228	energy conversion	1212:1228	energy conversion	1212:1228	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	8	55	theme	biological	1110:1119	arg1	processes					1121:1129	biological processes	1110:1129	biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion	1110:1228	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	8	55	theme	biological	1110:1119	arg1	metabolism					1164:1173	metabolism	1164:1173	metabolism	1164:1173	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	8	55	theme	biological	1110:1119	arg1	proliferation					1149:1161	bacterial proliferation	1139:1161	bacterial proliferation	1139:1161	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	2	56	theme	target	275:280	arg1	pollutants					282:291	target pollutants	275:291	target pollutants	275:291	Green and economical pollution management methods which reusing bio-waste as biostimulant to effectively improve the removal of target pollutants are receiving more and more attention.
37178747	7	57	theme	extracellular	945:957	arg1	substances					969:978	extracellular polymeric substances	945:978	extracellular polymeric substances	945:978	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	4	58	theme	transcriptomics	604:618	arg1	terms					570:574	terms	570:574	terms of both cell physiology and transcriptomics	570:618	Strain ZY1 in terms of both cell physiology and transcriptomics.
37178747	3	59	theme	Acinetobacter	538:550	arg1	sp					552:553	strain Acinetobacter sp	531:553	strain Acinetobacter sp	531:553	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	7	60	theme	substances	969:978	arg1	level					905:909	the level	901:909	the level of electron transfer activity and extracellular polymeric substances secretion	901:988	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	1	61	theme	bio-waste	130:138	arg1	reuse					140:144	A bio-waste reuse	128:144	A bio-waste reuse	128:144	stimulated by Lactobacillus plantarum fermentation waste: A bio-waste reuse.
37178747	7	62	dep	activity	932:939	arg1	secretion					980:988	secretion	980:988	secretion	980:988	It also significantly increased the level of electron transfer activity and extracellular polymeric substances secretion and improved the metabolic activity of the strain.
37178747	6	63	dep	%	859:859	arg1	to					861:862	to	861:862	to	861:862	The biostimulant maintained the morphology of strain, reduced the level of reactive oxygen species, and recovered the cell membrane permeability from 39% to 22%.
37178747	8	64	theme	transcriptome	1045:1057	arg1	results					1059:1065	The transcriptome results	1041:1065	The transcriptome results	1041:1065	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	9	65	from	reuse	1287:1291	arg1	methods					1341:1347	biostimulation methods	1326:1347	biostimulation methods	1326:1347	This study provided new insights and references for the reuse of fermentation waste streams in biostimulation methods.
37178747	0	66	theme	Acinetobacter	52:64	arg1	sp					66:67	Acinetobacter sp	52:67	Acinetobacter sp	52:67	Exploration of the mechanism of 2-CP degradation by Acinetobacter sp.
37178747	3	67	from	effect	446:451	arg1	degradation					491:501	the degradation	487:501	the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp	487:553	In this study, Lactobacillus plantarum fermentation waste solution (LPS) was used to investigate its facilitative effect and the stimulation mechanisms on the degradation of 2-chlorophenol (2-CP) by strain Acinetobacter sp.
37178747	8	68	theme	structure	1185:1193	arg1	composition					1195:1205	membrane structure composition	1176:1205	membrane structure composition	1176:1205	The transcriptome results revealed the stimulation of LPS to promote biological processes such as bacterial proliferation, metabolism, membrane structure composition, and energy conversion.
37178747	5	69	dep	%	684:684	arg1	to					678:679	to	678:679	to	678:679	The degradation efficiency of 2-CP was improved from 60% to >80% under LPS treatment.
36176579	0	0	theme	membrane	72:79	arg1	permeability					81:92	membrane permeability	72:92	membrane permeability	72:92	Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability.
36176579	5	1	from	defect	755:760	arg1	Cnm					765:767	Cnm	765:767	Cnm	765:767	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	7	2	theme	particular	1350:1359	arg1	antibiotics					1361:1371	particular antibiotics	1350:1371	particular antibiotics	1350:1371	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	2	theme	particular	1350:1359	arg1	bacitracin					1384:1393	bacitracin	1384:1393	bacitracin	1384:1393	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	2	theme	particular	1350:1359	arg1	chloramphenicol					1399:1413	chloramphenicol	1399:1413	chloramphenicol	1399:1413	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	8	3	theme	biological	1623:1632	arg1	properties					1634:1643	several biological properties	1615:1643	several biological properties related to membrane permeability	1615:1676	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	5	4	theme	strains	729:735	arg1	sequencing					711:720	Deep RNA sequencing	702:720	Deep RNA sequencing of the strains	702:735	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	3	5	theme	Cnm	478:480	arg1	properties					464:473	the properties	460:473	the properties of Cnm	460:480	In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm.
36176579	6	6	theme	Cnm-defective	942:954	arg1	strain					956:961	the Cnm-defective strain	938:961	the Cnm-defective strain	938:961	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	7	7	theme	Cnm-defective	1324:1336	arg1	strain					1338:1343	the Cnm-defective strain	1320:1343	the Cnm-defective strain	1320:1343	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	8	8	located	located	1511:1517	arg2	Cnm					1504:1506	S. mutans Cnm	1494:1506	S. mutans Cnm	1494:1506	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	8	8	located	located	1511:1517	arg1	surface					1531:1537	the cell surface	1522:1537	the cell surface	1522:1537	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	7	9	theme	complemented	1447:1458	arg1	strains					1460:1466	complemented strains	1447:1466	complemented strains	1447:1466	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	8	10	theme	related	1645:1651	arg1	properties					1634:1643	several biological properties	1615:1643	several biological properties related to membrane permeability	1615:1676	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	6	11	theme	glucan	1113:1118	arg1	enzymes					1130:1136	glucan synthesis enzymes	1113:1136	glucan synthesis enzymes	1113:1136	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	6	12	dep	parental	991:998	arg1	the					987:989	the	987:989	the	987:989	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	4	13	theme	electron	552:559	arg1	microscopy					561:570	electron microscopy	552:570	electron microscopy	552:570	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	5	14	theme	Deep	702:705	arg1	sequencing					711:720	Deep RNA sequencing	702:720	Deep RNA sequencing of the strains	702:735	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	6	15	dep	increased	963:971	arg1	compared					973:980	compared	973:980	increased compared with the parental and complemented strains	963:1023	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	6	16	theme	complemented	1004:1015	arg1	strains					1017:1023	complemented strains	1004:1023	complemented strains	1004:1023	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	4	17	theme	cell	632:635	arg1	surface					637:643	the cell surface	628:643	the cell surface	628:643	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	5	18	theme	related	813:819	arg1	genes					807:811	many genes	802:811	many genes related to ABC transporters and cell-surface proteins	802:865	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	5	19	theme	RNA	707:709	arg1	sequencing					711:720	Deep RNA sequencing	702:720	Deep RNA sequencing of the strains	702:735	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	6	20	theme	elevated	1075:1082	arg1	expression					1084:1093	elevated expression	1075:1093	elevated expression of genes encoding glucan synthesis enzymes	1075:1136	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	2	21	theme	collagen	248:255	arg1	binding					257:263	collagen binding	248:263	collagen binding	248:263	The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown.
36176579	1	22	theme	major	171:175	arg1	pathogen					177:184	a major pathogen	169:184	a major pathogen of dental caries	169:201	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	1	22	theme	major	171:175	arg1	mutans					109:114	Streptococcus mutans	95:114	Streptococcus mutans	95:114	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	7	23	contain	had	1271:1273	arg1	bacitracin					1239:1248	bacitracin	1239:1248	bacitracin	1239:1248	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	23	contain	had	1271:1273	arg2	concentration					1302:1314	a lower minimum inhibitory concentration	1275:1314	a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol,	1275:1414	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	23	contain	had	1271:1273	arg1	chloramphenicol					1254:1268	chloramphenicol	1254:1268	chloramphenicol	1254:1268	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	23	contain	had	1271:1273	arg1	antibiotics					1216:1226	Particular antibiotics	1205:1226	Particular antibiotics	1205:1226	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	24	theme	lower	1277:1281	arg1	concentration					1302:1314	a lower minimum inhibitory concentration	1275:1314	a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol,	1275:1414	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	6	25	theme	synthesis	1120:1128	arg1	enzymes					1130:1136	glucan synthesis enzymes	1113:1136	glucan synthesis enzymes	1113:1136	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	8	26	theme	cell	1526:1529	arg1	surface					1531:1537	the cell surface	1522:1537	the cell surface	1522:1537	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	3	27	theme	isogenic	345:352	arg1	mutant					354:359	a Cnm-deficient isogenic mutant	329:359	a Cnm-deficient isogenic mutant	329:359	In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm.
36176579	4	28	theme	surface	517:523	arg1	structure					525:533	the cell surface structure	508:533	the cell surface structure	508:533	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	4	29	theme	cell	678:681	arg1	structure					691:699	a protruding cell surface structure	665:699	a protruding cell surface structure	665:699	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	2	30	theme	biological	280:289	arg1	functions					291:299	its other biological functions	270:299	its other biological functions	270:299	The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown.
36176579	3	31	theme	Cnm-deficient	331:343	arg1	mutant					354:359	a Cnm-deficient isogenic mutant	329:359	a Cnm-deficient isogenic mutant	329:359	In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm.
36176579	4	32	theme	cell	512:515	arg1	structure					525:533	the cell surface structure	508:533	the cell surface structure	508:533	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	6	33	theme	polysaccharides	1188:1202	arg1	production					1160:1169	increased production	1150:1169	increased production of extracellular polysaccharides	1150:1202	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	2	34	theme	other	274:278	arg1	functions					291:299	its other biological functions	270:299	its other biological functions	270:299	The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown.
36176579	1	35	theme	dental	189:194	arg1	caries					196:201	dental caries	189:201	dental caries	189:201	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	0	36	theme	Streptococcus	7:19	arg1	mutans					21:26	Streptococcus mutans	7:26	Streptococcus mutans	7:26	Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability.
36176579	8	37	theme	observed	1558:1565	arg1	surface					1583:1589	the observed protruding cell surface	1554:1589	the observed protruding cell surface	1554:1589	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	4	38	located	localized	615:623	arg2	Cnm					597:599	Cnm	597:599	Cnm	597:599	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	4	38	located	localized	615:623	arg1	surface					637:643	the cell surface	628:643	the cell surface	628:643	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	6	39	theme	genes	1098:1102	arg1	expression					1084:1093	elevated expression	1075:1093	elevated expression of genes encoding glucan synthesis enzymes	1075:1136	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	1	40	theme	caries	196:201	arg1	pathogen					177:184	a major pathogen	169:184	a major pathogen of dental caries	169:201	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	1	40	theme	caries	196:201	arg1	mutans					109:114	Streptococcus mutans	95:114	Streptococcus mutans	95:114	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	7	41	theme	minimum	1283:1289	arg1	concentration					1302:1314	a lower minimum inhibitory concentration	1275:1314	a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol,	1275:1414	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	4	42	theme	structure	525:533	arg1	comparison					494:503	comparison	494:503	comparison of the cell surface structure	494:533	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	5	43	theme	many	802:805	arg1	genes					807:811	many genes	802:811	many genes related to ABC transporters and cell-surface proteins	802:865	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	3	44	theme	S.	425:426	arg1	strain					435:440	a Cnm-positive S. mutans strain	410:440	a Cnm-positive S. mutans strain to help determine the properties of Cnm	410:480	In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm.
36176579	6	45	theme	biofilm	1034:1040	arg1	thinner					1056:1062	thinner	1056:1062	thinner	1056:1062	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	6	45	theme	biofilm	1034:1040	arg1	structure					1042:1050	the biofilm structure	1030:1050	the biofilm structure	1030:1050	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	8	46	theme	cell	1578:1581	arg1	surface					1583:1589	the observed protruding cell surface	1554:1589	the observed protruding cell surface	1554:1589	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	5	47	theme	ABC	824:826	arg1	transporters					828:839	ABC transporters	824:839	ABC transporters	824:839	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	0	48	theme	mutans	21:26	arg1	Cnm					0:2	Cnm	0:2	Cnm of Streptococcus mutans	0:26	Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability.
36176579	3	49	theme	mutans	428:433	arg1	strain					435:440	a Cnm-positive S. mutans strain	410:440	a Cnm-positive S. mutans strain to help determine the properties of Cnm	410:480	In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm.
36176579	0	50	theme	cell	45:48	arg1	structure					58:66	cell surface structure	45:66	cell surface structure	45:66	Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability.
36176579	8	51	theme	protruding	1567:1576	arg1	surface					1583:1589	the observed protruding cell surface	1554:1589	the observed protruding cell surface	1554:1589	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	6	52	theme	biofilm	920:926	arg1	biofilm					920:926	biofilm	920:926	biofilm	920:926	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	6	52	theme	biofilm	920:926	arg1	amount					910:915	The amount	906:915	The amount of biofilm formed by the Cnm-defective strain	906:961	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	8	53	theme	mutans	1497:1502	arg1	Cnm					1504:1506	S. mutans Cnm	1494:1506	S. mutans Cnm	1494:1506	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	4	54	theme	protruding	667:676	arg1	structure					691:699	a protruding cell surface structure	665:699	a protruding cell surface structure	665:699	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	3	55	theme	complementation	367:381	arg1	strain					383:388	a complementation strain	365:388	a complementation strain	365:388	In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm.
36176579	8	56	theme	S.	1494:1495	arg1	Cnm					1504:1506	S. mutans Cnm	1494:1506	S. mutans Cnm	1494:1506	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	2	57	theme	protein	208:214	arg1	Cnm					216:218	The protein Cnm	204:218	The protein Cnm of S. mutans	204:231	The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown.
36176579	5	58	theme	few	876:878	arg1	genes					880:884	a few genes	874:884	a few genes	874:884	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	8	59	theme	membrane	1656:1663	arg1	permeability					1665:1676	membrane permeability	1656:1676	membrane permeability	1656:1676	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	6	60	theme	increased	1150:1158	arg1	production					1160:1169	increased production	1150:1169	increased production of extracellular polysaccharides	1150:1202	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	8	61	theme	several	1615:1621	arg1	properties					1634:1643	several biological properties	1615:1643	several biological properties related to membrane permeability	1615:1676	Our results suggest that S. mutans Cnm is located on the cell surface, gives rise to the observed protruding cell surface, and is associated with several biological properties related to membrane permeability.
36176579	6	62	theme	extracellular	1174:1186	arg1	polysaccharides					1188:1202	extracellular polysaccharides	1174:1202	extracellular polysaccharides	1174:1202	The amount of biofilm formed by the Cnm-defective strain increased compared with the parental and complemented strains, but the biofilm structure was thinner because of elevated expression of genes encoding glucan synthesis enzymes, leading to increased production of extracellular polysaccharides.
36176579	1	63	theme	Gram-positive	119:131	arg1	bacterium					155:163	a Gram-positive facultative anaerobic bacterium	117:163	a Gram-positive facultative anaerobic bacterium	117:163	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	1	63	theme	Gram-positive	119:131	arg1	mutans					109:114	Streptococcus mutans	95:114	Streptococcus mutans	95:114	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	7	64	dep	parental	1434:1441	arg1	the					1430:1432	the	1430:1432	the	1430:1432	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	5	65	theme	upregulated	776:786	arg1	expression					788:797	upregulated expression	776:797	upregulated expression of many genes related to ABC transporters and cell-surface proteins	776:865	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	1	66	theme	facultative	133:143	arg1	bacterium					155:163	a Gram-positive facultative anaerobic bacterium	117:163	a Gram-positive facultative anaerobic bacterium	117:163	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	1	66	theme	facultative	133:143	arg1	mutans					109:114	Streptococcus mutans	95:114	Streptococcus mutans	95:114	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	4	67	theme	surface	683:689	arg1	structure					691:699	a protruding cell surface structure	665:699	a protruding cell surface structure	665:699	Initially, comparison of the cell surface structure was performed by electron microscopy, which demonstrated that Cnm appears to be localized on the cell surface and associated with a protruding cell surface structure.
36176579	7	68	theme	Particular	1205:1214	arg1	bacitracin					1239:1248	bacitracin	1239:1248	bacitracin	1239:1248	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	68	theme	Particular	1205:1214	arg1	chloramphenicol					1254:1268	chloramphenicol	1254:1268	chloramphenicol	1254:1268	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	7	68	theme	Particular	1205:1214	arg1	antibiotics					1216:1226	Particular antibiotics	1205:1226	Particular antibiotics	1205:1226	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	5	69	theme	genes	807:811	arg1	expression					788:797	upregulated expression	776:797	upregulated expression of many genes related to ABC transporters and cell-surface proteins	776:865	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
36176579	7	70	theme	inhibitory	1291:1300	arg1	concentration					1302:1314	a lower minimum inhibitory concentration	1275:1314	a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol,	1275:1414	Particular antibiotics, including bacitracin and chloramphenicol, had a lower minimum inhibitory concentration for the Cnm-defective strain than particular antibiotics, including bacitracin and chloramphenicol, compared with the parental and complemented strains.
36176579	1	71	theme	anaerobic	145:153	arg1	bacterium					155:163	a Gram-positive facultative anaerobic bacterium	117:163	a Gram-positive facultative anaerobic bacterium	117:163	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	1	71	theme	anaerobic	145:153	arg1	mutans					109:114	Streptococcus mutans	95:114	Streptococcus mutans	95:114	Streptococcus mutans, a Gram-positive facultative anaerobic bacterium, is a major pathogen of dental caries.
36176579	3	72	theme	Cnm-positive	412:423	arg1	strain					435:440	a Cnm-positive S. mutans strain	410:440	a Cnm-positive S. mutans strain to help determine the properties of Cnm	410:480	In this study, a Cnm-deficient isogenic mutant and a complementation strain were generated from a Cnm-positive S. mutans strain to help determine the properties of Cnm.
36176579	0	73	theme	surface	50:56	arg1	structure					58:66	cell surface structure	45:66	cell surface structure	45:66	Cnm of Streptococcus mutans is important for cell surface structure and membrane permeability.
36176579	2	74	theme	mutans	226:231	arg1	Cnm					216:218	The protein Cnm	204:218	The protein Cnm of S. mutans	204:231	The protein Cnm of S. mutans is involved in collagen binding, but its other biological functions are unknown.
36176579	5	75	theme	cell-surface	845:856	arg1	proteins					858:865	cell-surface proteins	845:865	cell-surface proteins	845:865	Deep RNA sequencing of the strains revealed that the defect in Cnm caused upregulated expression of many genes related to ABC transporters and cell-surface proteins, while a few genes were downregulated.
37353546	5	0	theme	successful	865:874	arg1	production					876:885	the successful production	861:885	the successful production of silver crystal, graphene oxidation and the blending of both components	861:959	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	10	1	from	research	1610:1617	arg1	fabrication					1626:1636	the fabrication	1622:1636	the fabrication of bioactive wound dressings	1622:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	1	2	theme	quality	257:263	arg1	life					265:268	patients' quality life	247:268	patients' quality life	247:268	An adequate wound dressing reduces time of healing, provides cost-effective care, thereby improving patients' quality life.
37353546	9	3	used	used	1356:1359	arg2	coli					1320:1323	Escherichia coli	1308:1323	Escherichia coli	1308:1323	Escherichia coli and Staphylococcus aureus were used to demonstrate the antibacterial bioactivity and L-929 fibroblast cells were utilized to visualize their biocompatibility.
37353546	9	3	used	used	1356:1359	arg2	aureus					1344:1349	Staphylococcus aureus	1329:1349	Staphylococcus aureus	1329:1349	Escherichia coli and Staphylococcus aureus were used to demonstrate the antibacterial bioactivity and L-929 fibroblast cells were utilized to visualize their biocompatibility.
37353546	10	4	theme	bioactive	1641:1649	arg1	dressings					1657:1665	bioactive wound dressings	1641:1665	bioactive wound dressings	1641:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	4	5	theme	graphene	679:686	arg1	GrOx					695:698	GrOx	695:698	GrOx	695:698	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	5	theme	graphene	679:686	arg1	oxide					688:692	the graphene oxide	675:692	the graphene oxide (GrOx)	675:699	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	5	6	theme	graphene	906:913	arg1	oxidation					915:923	graphene oxidation	906:923	graphene oxidation	906:923	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	10	7	theme	fiber	1560:1564	arg1	network					1566:1572	specific fiber network	1551:1572	specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings	1551:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	4	8	theme	family	740:745	arg1	onion					759:763	onion	759:763	onion	759:763	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	8	theme	family	740:745	arg1	members					710:716	two members	706:716	two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials	706:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	8	theme	family	740:745	arg1	garlic					748:753	garlic	748:753	garlic	748:753	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	9	theme	series	643:648	arg1	result					631:636	the result	627:636	the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials	627:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	9	theme	series	643:648	arg1	nanocomposite					601:613	The nanocomposite	597:613	The nanocomposite obtained	597:622	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	0	10	theme	potential	103:111	arg1	use					113:115	its potential use	99:115	its potential use in bioactive wound dressings	99:144	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	9	11	theme	fibroblast	1416:1425	arg1	cells					1427:1431	L-929 fibroblast cells	1410:1431	L-929 fibroblast cells	1410:1431	Escherichia coli and Staphylococcus aureus were used to demonstrate the antibacterial bioactivity and L-929 fibroblast cells were utilized to visualize their biocompatibility.
37353546	7	12	dep	thermal	1194:1200	arg1	better					1187:1192	better	1187:1192	better	1187:1192	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	3	13	from	corn	532:535	arg1	chitosan					518:525	chitosan	518:525	chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum)	518:594	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	4	14	theme	Amaryllidaceae	725:738	arg1	family					740:745	the Amaryllidaceae family	721:745	the Amaryllidaceae family	721:745	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	9	15	used	utilized	1438:1445	arg2	cells					1427:1431	L-929 fibroblast cells	1410:1431	L-929 fibroblast cells	1410:1431	Escherichia coli and Staphylococcus aureus were used to demonstrate the antibacterial bioactivity and L-929 fibroblast cells were utilized to visualize their biocompatibility.
37353546	8	16	theme	successful	1258:1267	arg1	production					1269:1278	the successful production	1254:1278	the successful production of the ChAgG nanocomposite	1254:1305	It was also confirmed the successful production of the ChAgG nanocomposite.
37353546	0	17	theme	bioactive	120:128	arg1	dressings					136:144	bioactive wound dressings	120:144	bioactive wound dressings	120:144	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	4	18	theme	different	795:803	arg1	materials					812:820	different sulfur materials	795:820	different sulfur materials	795:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	1	19	theme	cost-effective	208:221	arg1	care					223:226	cost-effective care	208:226	cost-effective care	208:226	An adequate wound dressing reduces time of healing, provides cost-effective care, thereby improving patients' quality life.
37353546	8	20	theme	ChAgG	1287:1291	arg1	nanocomposite					1293:1305	the ChAgG nanocomposite	1283:1305	the ChAgG nanocomposite	1283:1305	It was also confirmed the successful production of the ChAgG nanocomposite.
37353546	4	21	theme	experiments	653:663	arg1	series					643:648	a series	641:648	a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials	641:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	2	22	theme	antimicrobial	379:391	arg1	oxide					448:452	chitosan/silver nanocrystals/graphene oxide	410:452	chitosan/silver nanocrystals/graphene oxide (ChAgG)	410:460	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	2	22	theme	antimicrobial	379:391	arg1	nanocomposite					393:405	an antimicrobial nanocomposite	376:405	an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG)	376:460	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	7	23	theme	nanocrystals	1146:1157	arg1	synthesis					1126:1134	the synthesis	1122:1134	the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties	1122:1229	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	6	24	dep	graphene	1007:1014	arg1	nanocrystals					1027:1038	nanocrystals	1027:1038	nanocrystals	1027:1038	The role of the chitosan as a binder between graphene and silver nanocrystals is proved.
37353546	2	25	theme	antimicrobial	274:286	arg1	bioactivity					288:298	An antimicrobial bioactivity	271:298	An antimicrobial bioactivity	271:298	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	3	26	dep	Allium	580:585	arg1	sativum					587:593	Allium sativum	580:593	Allium sativum	580:593	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	10	27	theme	current	1590:1596	arg1	research					1610:1617	current challenging research	1590:1617	current challenging research in the fabrication of bioactive wound dressings	1590:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	1	28	dep	dressing	165:172	arg1	reduces					174:180	reduces	174:180	reduces time of healing	174:196	An adequate wound dressing reduces time of healing, provides cost-effective care, thereby improving patients' quality life.
37353546	1	29	theme	healing	190:196	arg1	time					182:185	time	182:185	time of healing	182:196	An adequate wound dressing reduces time of healing, provides cost-effective care, thereby improving patients' quality life.
37353546	0	30	theme	Antibacterial	0:12	arg1	nanocomposite					14:26	Antibacterial nanocomposite	0:26	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.	0:145	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	0	30	theme	Antibacterial	0:12	arg1	development					83:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	10	31	theme	challenging	1598:1608	arg1	research					1610:1617	current challenging research	1590:1617	current challenging research in the fabrication of bioactive wound dressings	1590:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	4	32	theme	sulfur	805:810	arg1	materials					812:820	different sulfur materials	795:820	different sulfur materials	795:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	5	33	theme	oxidation	915:923	arg1	production					876:885	the successful production	861:885	the successful production of silver crystal, graphene oxidation and the blending of both components	861:959	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	9	34	theme	L-929	1410:1414	arg1	cells					1427:1431	L-929 fibroblast cells	1410:1431	L-929 fibroblast cells	1410:1431	Escherichia coli and Staphylococcus aureus were used to demonstrate the antibacterial bioactivity and L-929 fibroblast cells were utilized to visualize their biocompatibility.
37353546	0	35	theme	chitosan/silver	31:45	arg1	development					83:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	10	36	theme	wound	1651:1655	arg1	dressings					1657:1665	bioactive wound dressings	1641:1665	bioactive wound dressings	1641:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	9	37	theme	antibacterial	1380:1392	arg1	bioactivity					1394:1404	the antibacterial bioactivity	1376:1404	the antibacterial bioactivity	1376:1404	Escherichia coli and Staphylococcus aureus were used to demonstrate the antibacterial bioactivity and L-929 fibroblast cells were utilized to visualize their biocompatibility.
37353546	3	38	theme	silver	547:552	arg1	nanocrystals					554:565	silver nanocrystals	547:565	silver nanocrystals from garlic (Allium sativum)	547:594	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	2	39	theme	nanocrystals/graphene	426:446	arg1	ChAgG					455:459	ChAgG	455:459	ChAgG	455:459	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	2	39	theme	nanocrystals/graphene	426:446	arg1	oxide					448:452	chitosan/silver nanocrystals/graphene oxide	410:452	chitosan/silver nanocrystals/graphene oxide (ChAgG)	410:460	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	2	40	theme	oxide	448:452	arg1	oxide					448:452	chitosan/silver nanocrystals/graphene oxide	410:452	chitosan/silver nanocrystals/graphene oxide (ChAgG)	410:460	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	2	40	theme	oxide	448:452	arg1	nanocomposite					393:405	an antimicrobial nanocomposite	376:405	an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG)	376:460	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	0	41	theme	wound	130:134	arg1	dressings					136:144	bioactive wound dressings	120:144	bioactive wound dressings	120:144	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	2	42	theme	chitosan/silver	410:424	arg1	ChAgG					455:459	ChAgG	455:459	ChAgG	455:459	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	2	42	theme	chitosan/silver	410:424	arg1	oxide					448:452	chitosan/silver nanocrystals/graphene oxide	410:452	chitosan/silver nanocrystals/graphene oxide (ChAgG)	410:460	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	0	43	theme	oxide	69:73	arg1	development					83:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	7	44	theme	crystallinity	1206:1218	arg1	properties					1220:1229	better thermal and crystallinity properties	1187:1229	properties	1220:1229	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	7	45	dep	⁓	1168:1168	arg1	to					1172:1173	to	1172:1173	to	1172:1173	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	0	46	theme	nanocrystals/graphene	47:67	arg1	oxide					69:73	nanocrystals/graphene oxide	47:73	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	0	46	theme	nanocrystals/graphene	47:67	arg1	ChAgG					76:80	ChAgG	76:80	ChAgG	76:80	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	5	47	theme	characterization	827:842	arg1	arrays					844:849	The characterization arrays	823:849	The characterization arrays	823:849	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	7	48	with	nanocrystals	1146:1157	arg1	thermal					1194:1200	thermal	1194:1200	thermal	1194:1200	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	7	48	with	nanocrystals	1146:1157	arg1	properties					1220:1229	better thermal and crystallinity properties	1187:1229	properties	1220:1229	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	3	49	theme	ChAgG	463:467	arg1	material					494:501	ChAgG nanostructured composite material	463:501	ChAgG nanostructured composite material	463:501	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	10	50	theme	ChAgG	1497:1501	arg1	nanomaterial					1503:1514	The proposed ChAgG nanomaterial	1484:1514	The proposed ChAgG nanomaterial	1484:1514	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	10	50	theme	ChAgG	1497:1501	arg1	useful					1524:1529	useful	1524:1529	useful	1524:1529	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	8	51	theme	nanocomposite	1293:1305	arg1	production					1269:1278	the successful production	1254:1278	the successful production of the ChAgG nanocomposite	1254:1305	It was also confirmed the successful production of the ChAgG nanocomposite.
37353546	3	52	theme	composite	484:492	arg1	material					494:501	ChAgG nanostructured composite material	463:501	ChAgG nanostructured composite material	463:501	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	4	53	dep	Allium	766:771	arg1	cebae					773:777	Allium cebae	766:777	Allium cebae	766:777	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	10	54	theme	proposed	1488:1495	arg1	nanomaterial					1503:1514	The proposed ChAgG nanomaterial	1484:1514	The proposed ChAgG nanomaterial	1484:1514	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	10	54	theme	proposed	1488:1495	arg1	useful					1524:1529	useful	1524:1529	useful	1524:1529	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	5	55	theme	silver	890:895	arg1	crystal					897:903	silver crystal	890:903	silver crystal	890:903	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	7	56	theme	optimal	1094:1100	arg1	source					1102:1107	an optimal source	1091:1107	an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties	1091:1229	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	7	56	theme	optimal	1094:1100	arg1	garlic					1081:1086	garlic	1081:1086	garlic	1081:1086	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	4	57	dep	members	710:716	arg1	Allium					766:771	Allium	766:771	Allium	766:771	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	57	dep	members	710:716	arg1	members					710:716	two members	706:716	two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials	706:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	57	dep	members	710:716	arg1	onion					759:763	onion	759:763	onion	759:763	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	57	dep	members	710:716	arg1	garlic					748:753	garlic	748:753	garlic	748:753	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	3	58	from	nanocrystals	554:565	arg1	chitosan					518:525	chitosan	518:525	chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum)	518:594	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	5	59	theme	components	950:959	arg1	crystal					897:903	silver crystal	890:903	silver crystal	890:903	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	5	59	theme	components	950:959	arg1	oxidation					915:923	graphene oxidation	906:923	graphene oxidation	906:923	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	5	59	theme	components	950:959	arg1	blending					933:940	the blending	929:940	the blending of both components	929:959	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	0	60	from	use	113:115	arg1	dressings					136:144	bioactive wound dressings	120:144	bioactive wound dressings	120:144	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	5	61	theme	blending	933:940	arg1	production					876:885	the successful production	861:885	the successful production of silver crystal, graphene oxidation and the blending of both components	861:959	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	1	62	theme	wound	159:163	arg1	dressing					165:172	An adequate wound dressing	147:172	An adequate wound dressing	147:172	An adequate wound dressing reduces time of healing, provides cost-effective care, thereby improving patients' quality life.
37353546	4	63	contain	contain	787:793	arg1	Allium					766:771	Allium	766:771	Allium	766:771	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	63	contain	contain	787:793	arg2	materials					812:820	different sulfur materials	795:820	different sulfur materials	795:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	63	contain	contain	787:793	arg1	onion					759:763	onion	759:763	onion	759:763	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	63	contain	contain	787:793	arg1	members					710:716	two members	706:716	two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials	706:820	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	4	63	contain	contain	787:793	arg1	garlic					748:753	garlic	748:753	garlic	748:753	The nanocomposite obtained is the result of a series of experiments combining the graphene oxide (GrOx) with two members of the Amaryllidaceae family; garlic and onion (Allium cebae), which contain different sulfur materials.
37353546	5	64	theme	crystal	897:903	arg1	production					876:885	the successful production	861:885	the successful production of silver crystal, graphene oxidation and the blending of both components	861:959	The characterization arrays confirmed the successful production of silver crystal, graphene oxidation and the blending of both components.
37353546	0	65	theme	development	83:93	arg1	nanocomposite					14:26	Antibacterial nanocomposite	0:26	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.	0:145	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	0	65	theme	development	83:93	arg1	development					83:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	chitosan/silver nanocrystals/graphene oxide (ChAgG) development	31:93	Antibacterial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG) development for its potential use in bioactive wound dressings.
37353546	10	66	theme	dressings	1657:1665	arg1	fabrication					1626:1636	the fabrication	1622:1636	the fabrication of bioactive wound dressings	1622:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
37353546	3	67	theme	nanostructured	469:482	arg1	material					494:501	ChAgG nanostructured composite material	463:501	ChAgG nanostructured composite material	463:501	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	1	68	theme	adequate	150:157	arg1	dressing					165:172	An adequate wound dressing	147:172	An adequate wound dressing	147:172	An adequate wound dressing reduces time of healing, provides cost-effective care, thereby improving patients' quality life.
37353546	3	69	from	garlic	572:577	arg1	nanocrystals					554:565	silver nanocrystals	547:565	silver nanocrystals from garlic (Allium sativum)	547:594	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	3	69	from	garlic	572:577	arg1	Ch					538:539	Ch	538:539	Ch	538:539	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	3	69	from	garlic	572:577	arg1	corn					532:535	corn	532:535	corn (Ch)	532:540	ChAgG nanostructured composite material is composed of chitosan from corn (Ch), and silver nanocrystals from garlic (Allium sativum).
37353546	6	70	theme	chitosan	978:985	arg1	role					966:969	The role	962:969	The role of the chitosan as a binder between graphene and silver nanocrystals	962:1038	The role of the chitosan as a binder between graphene and silver nanocrystals is proved.
37353546	7	71	theme	silver	1139:1144	arg1	AgNCs					1160:1164	AgNCs	1160:1164	AgNCs	1160:1164	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	7	71	theme	silver	1139:1144	arg1	⁓					1168:1168	⁓ 2 to 10 nm	1168:1179	⁓ 2 to 10 nm	1168:1179	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	7	71	theme	silver	1139:1144	arg1	nanocrystals					1146:1157	silver nanocrystals	1139:1157	silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties	1139:1229	Moreover, the study discusses garlic as an optimal source that permits the synthesis of silver nanocrystals (AgNCs) (⁓ 2 to 10 nm) with better thermal and crystallinity properties.
37353546	2	72	theme	work	358:361	arg1	objective					340:348	the objective	336:348	the objective of this work	336:361	An antimicrobial bioactivity is always desired, for that reason, the objective of this work is to design an antimicrobial nanocomposite of chitosan/silver nanocrystals/graphene oxide (ChAgG).
37353546	10	73	theme	specific	1551:1558	arg1	network					1566:1572	specific fiber network	1551:1572	specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings	1551:1665	The proposed ChAgG nanomaterial will be useful for functionalizing specific fiber network that represents current challenging research in the fabrication of bioactive wound dressings.
35392922	4	0	theme	biochemical	910:920	arg1	characterization					937:952	The physiochemical, biochemical and structural characterization	890:952	The physiochemical, biochemical and structural characterization of generated molecules	890:975	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	2	1	theme	efficacious	595:605	arg1	extract					446:452	plant extract	440:452	plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	440:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	1	theme	efficacious	595:605	arg1	agents/nanocomposites					618:638	efficacious fungicidal agents/nanocomposites	595:638	efficacious fungicidal agents/nanocomposites	595:638	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	2	theme	fungicides	373:382	arg1	demands					431:437	global demands	424:437	global demands	424:437	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	2	theme	fungicides	373:382	arg1	substitutions					333:345	The substitutions	329:345	The substitutions of chemical and synthetic fungicides with effectual natural alternatives	329:418	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	4	3	theme	structural	926:935	arg1	characterization					937:952	The physiochemical, biochemical and structural characterization	890:952	The physiochemical, biochemical and structural characterization of generated molecules	890:975	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	6	4	theme	synthesized	1353:1363	arg1	agents/composites					1365:1381	synthesized agents/composites	1353:1381	synthesized agents/composites	1353:1381	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	10	5	theme	fungal	1939:1944	arg1	mycelia					1946:1952	fungal mycelia	1939:1952	fungal mycelia	1939:1952	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	6	6	from	bonding	1342:1348	arg1	agents/composites					1365:1381	synthesized agents/composites	1353:1381	synthesized agents/composites	1353:1381	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	4	7	theme	physiochemical	894:907	arg1	characterization					937:952	The physiochemical, biochemical and structural characterization	890:952	The physiochemical, biochemical and structural characterization of generated molecules	890:975	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	7	8	theme	85.17 nm	1512:1519	arg1	diameters					1526:1534	82.72, 9.41 and 85.17 nm mean diameters	1496:1534	diameters	1526:1534	The nanoparticles had 82.72, 9.41 and 85.17 nm mean diameters for NCT, PPE/SeNPs and NCT/PPE/SeNPs nanocomposites, respectively.
35392922	9	9	theme	fungicidal	1874:1883	arg1	action					1885:1890	the fungicidal action	1870:1890	the fungicidal action of standard fungicide	1870:1912	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	6	10	theme	biochemical	1435:1445	arg1	interactions					1460:1471	both biochemical and physical interactions	1430:1471	both biochemical and physical interactions	1430:1471	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	1	11	theme	fungal	224:229	arg1	worldwide					238:246	fungal decays worldwide	224:246	fungal decays worldwide	224:246	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	4	12	theme	infra-red	998:1006	arg1	imaging					1090:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	7	13	theme	82.72	1496:1500	arg1	diameters					1526:1534	82.72, 9.41 and 85.17 nm mean diameters	1496:1534	diameters	1526:1534	The nanoparticles had 82.72, 9.41 and 85.17 nm mean diameters for NCT, PPE/SeNPs and NCT/PPE/SeNPs nanocomposites, respectively.
35392922	8	14	contain	had	1621:1623	arg2	attributes					1675:1684	good distribution attributes	1657:1684	good distribution attributes	1657:1684	The nanoparticles had homogenous spherical shapes and good distribution attributes.
35392922	8	14	contain	had	1621:1623	arg1	nanoparticles					1607:1619	The nanoparticles	1603:1619	The nanoparticles	1603:1619	The nanoparticles had homogenous spherical shapes and good distribution attributes.
35392922	8	14	contain	had	1621:1623	arg2	shapes					1646:1651	homogenous spherical shapes	1625:1651	homogenous spherical shapes	1625:1651	The nanoparticles had homogenous spherical shapes and good distribution attributes.
35392922	11	15	theme	infection	2162:2170	arg1	signs					2172:2176	the green mold infection signs	2147:2176	the green mold infection signs	2147:2176	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	9	16	theme	fungicide	1904:1912	arg1	action					1885:1890	the fungicidal action	1870:1890	the fungicidal action of standard fungicide	1870:1912	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	12	17	theme	existing	2561:2568	arg1	infections					2570:2579	their existing infections	2555:2579	their existing infections	2555:2579	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	11	18	theme	coating	2243:2249	arg1	solutions					2251:2259	based coating solutions	2237:2259	based coating solutions	2237:2259	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	3	19	theme	gelation	802:809	arg1	method					811:816	ionic gelation method	796:816	ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells	796:887	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	5	20	theme	Antifungal	1099:1108	arg1	potentialities					1110:1123	Antifungal potentialities	1099:1123	Antifungal potentialities	1099:1123	Antifungal potentialities were investigated in vitro and in infected fruits with P. digitatum by applying NCT nanocomposites-based edible coating.
35392922	6	21	contain	have	1425:1428	arg1	bonding					1342:1348	the molecular bonding	1328:1348	the molecular bonding in synthesized agents/composites	1328:1381	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	6	21	contain	have	1425:1428	arg2	interactions					1460:1471	both biochemical and physical interactions	1430:1471	both biochemical and physical interactions	1430:1471	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	4	22	theme	molecules	967:975	arg1	characterization					937:952	The physiochemical, biochemical and structural characterization	890:952	The physiochemical, biochemical and structural characterization of generated molecules	890:975	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	6	23	theme	NCT	1297:1299	arg1	synthesis					1258:1266	The synthesis	1254:1266	The synthesis of PPE-synthesized SeNPs and NCT	1254:1299	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	3	24	theme	white	845:849	arg1	shells					882:887	white prawn (Fenneropenaeus indicus) shells	845:887	white prawn (Fenneropenaeus indicus) shells	845:887	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	11	25	theme	100	2196:2198	arg1	%					2199:2199	91.7, 95.4 and 100%	2181:2199	%	2199:2199	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	10	26	with	treatment	1926:1934	arg1	nanocomposite					1973:1985	NCT/PPE/SeNPs nanocomposite	1959:1985	NCT/PPE/SeNPs nanocomposite	1959:1985	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	6	27	theme	SeNPs	1287:1291	arg1	synthesis					1258:1266	The synthesis	1254:1266	The synthesis of PPE-synthesized SeNPs and NCT	1254:1299	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	5	28	with	fruits	1168:1173	arg1	digitatum					1183:1191	P. digitatum	1180:1191	P. digitatum	1180:1191	Antifungal potentialities were investigated in vitro and in infected fruits with P. digitatum by applying NCT nanocomposites-based edible coating.
35392922	3	29	dep	extracted	709:717	arg1	whereas					765:771	whereas	765:771	whereas	765:771	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	1	30	theme	Citrus	168:173	arg1	production					175:184	BACKGROUND Citrus production	157:184	BACKGROUND Citrus production	157:184	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	3	31	theme	indicus	873:879	arg1	shells					882:887	white prawn (Fenneropenaeus indicus) shells	845:887	white prawn (Fenneropenaeus indicus) shells	845:887	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	11	32	with	coating	2081:2087	arg1	coatings					2130:2137	NCT-based edible coatings	2113:2137	NCT-based edible coatings	2113:2137	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	2	33	theme	chitosan	548:555	arg1	NCT					572:574	NCT	572:574	NCT	572:574	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	33	theme	chitosan	548:555	arg1	nanoparticles					557:569	PPE (PPE/SeNPs) and chitosan nanoparticles	528:569	PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	528:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	8	34	theme	homogenous	1625:1634	arg1	shapes					1646:1651	homogenous spherical shapes	1625:1651	homogenous spherical shapes	1625:1651	The nanoparticles had homogenous spherical shapes and good distribution attributes.
35392922	0	35	theme	edible	107:112	arg1	coatings					114:121	edible coatings	107:121	edible coatings for citrus green mold protection	107:154	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	5	36	theme	infected	1159:1166	arg1	fruits					1168:1173	infected fruits	1159:1173	infected fruits with P. digitatum	1159:1191	Antifungal potentialities were investigated in vitro and in infected fruits with P. digitatum by applying NCT nanocomposites-based edible coating.
35392922	12	37	theme	postharvest	2465:2475	arg1	pathogen					2484:2491	postharvest fungal pathogen	2465:2491	postharvest fungal pathogen	2465:2491	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	11	38	theme	orange	2101:2106	arg1	coating					2081:2087	The coating	2077:2087	The coating of infected orange with NCT-based edible coatings	2077:2137	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	6	39	theme	physical	1451:1458	arg1	interactions					1460:1471	both biochemical and physical interactions	1430:1471	both biochemical and physical interactions	1430:1471	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	2	40	theme	selenium	500:507	arg1	nanoparticles					509:521	biosynthesized selenium nanoparticles	485:521	biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	485:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	40	theme	selenium	500:507	arg1	peels					472:476	pomegranates peels	459:476	pomegranates peels (PPE)	459:482	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	9	41	theme	entire	1691:1696	arg1	agents/nanocomposites					1698:1718	The entire agents/nanocomposites	1687:1718	The entire agents/nanocomposites	1687:1718	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	8	42	theme	good	1657:1660	arg1	attributes					1675:1684	good distribution attributes	1657:1684	good distribution attributes	1657:1684	The nanoparticles had homogenous spherical shapes and good distribution attributes.
35392922	12	43	theme	effectual	2411:2419	arg1	coatings					2443:2450	effectual antifungal and edible coatings	2411:2450	effectual antifungal and edible coatings	2411:2450	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	0	44	theme	Antifungal	0:9	arg1	application					11:21	Antifungal application	0:21	Antifungal application of biosynthesized selenium	0:48	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	9	45	theme	fungicidal	1737:1746	arg1	potentialities					1748:1761	potent fungicidal potentialities	1730:1761	potent fungicidal potentialities	1730:1761	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	4	46	theme	charge	1047:1052	arg1	assessment					1054:1063	charge assessment and electron microscopes	1047:1088	assessment	1054:1063	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	11	47	theme	NCT-based	2113:2121	arg1	coatings					2130:2137	NCT-based edible coatings	2113:2137	NCT-based edible coatings	2113:2137	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	2	48	theme	plant	440:444	arg1	extract					446:452	plant extract	440:452	plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	440:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	48	theme	plant	440:444	arg1	agents/nanocomposites					618:638	efficacious fungicidal agents/nanocomposites	595:638	efficacious fungicidal agents/nanocomposites	595:638	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	0	49	theme	selenium	41:48	arg1	application					11:21	Antifungal application	0:21	Antifungal application of biosynthesized selenium	0:48	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	5	50	theme	nanocomposites-based	1209:1228	arg1	coating					1237:1243	NCT nanocomposites-based edible coating	1205:1243	NCT nanocomposites-based edible coating	1205:1243	Antifungal potentialities were investigated in vitro and in infected fruits with P. digitatum by applying NCT nanocomposites-based edible coating.
35392922	2	51	theme	PPE	528:530	arg1	NCT					572:574	NCT	572:574	NCT	572:574	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	51	theme	PPE	528:530	arg1	nanoparticles					557:569	PPE (PPE/SeNPs) and chitosan nanoparticles	528:569	PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	528:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	52	theme	synthetic	363:371	arg1	fungicides					373:382	chemical and synthetic fungicides	350:382	chemical and synthetic fungicides with effectual natural alternatives	350:418	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	9	53	theme	digitatum	1773:1781	arg1	isolates					1783:1790	P. digitatum isolates	1770:1790	P. digitatum isolates	1770:1790	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	12	54	theme	innovative	2326:2335	arg1	NCT/PPE/SeNPs					2352:2364	their innovative nanocomposites NCT/PPE/SeNPs	2320:2364	their innovative nanocomposites NCT/PPE/SeNPs	2320:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	12	54	theme	innovative	2326:2335	arg1	CONCLUSIONS					2276:2286	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	2	55	theme	chemical	350:357	arg1	fungicides					373:382	chemical and synthetic fungicides	350:382	chemical and synthetic fungicides with effectual natural alternatives	350:418	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	3	56	from	granatum	696:703	arg1	PPE					680:682	METHOD PPE	673:682	METHOD PPE from Punica granatum	673:703	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	10	57	theme	treatment	2066:2074	arg1	12 h					2058:2061	12 h	2058:2061	12 h of treatment	2058:2074	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	2	58	theme	natural	399:405	arg1	alternatives					407:418	effectual natural alternatives	389:418	effectual natural alternatives	389:418	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	1	59	theme	green	253:257	arg1	disastrous					317:326	disastrous	317:326	disastrous	317:326	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	1	59	theme	green	253:257	arg1	infection					264:272	the green mold infection	249:272	the green mold infection by Penicillium digitatum	249:297	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	12	60	from	invasion	2526:2533	arg1	protection					2504:2513	protection	2504:2513	protection from their invasion	2504:2533	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	7	61	contain	had	1492:1494	arg2	diameters					1526:1534	82.72, 9.41 and 85.17 nm mean diameters	1496:1534	diameters	1526:1534	The nanoparticles had 82.72, 9.41 and 85.17 nm mean diameters for NCT, PPE/SeNPs and NCT/PPE/SeNPs nanocomposites, respectively.
35392922	7	61	contain	had	1492:1494	arg1	nanoparticles					1478:1490	The nanoparticles	1474:1490	The nanoparticles	1474:1490	The nanoparticles had 82.72, 9.41 and 85.17 nm mean diameters for NCT, PPE/SeNPs and NCT/PPE/SeNPs nanocomposites, respectively.
35392922	10	62	theme	hyphae	2044:2049	arg1	lysis					2005:2009	remarkable lysis	1994:2009	remarkable lysis	1994:2009	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	10	62	theme	hyphae	2044:2049	arg1	deformations					2015:2026	deformations	2015:2026	deformations of P. digitatum hyphae within 12 h of treatment	2015:2074	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	12	63	dep	CONCLUSIONS	2276:2286	arg1	NCT					2288:2290	NCT	2288:2290	NCT	2288:2290	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	12	63	dep	CONCLUSIONS	2276:2286	arg1	SeNPs					2309:2313	PPE-synthesized SeNPs	2293:2313	PPE-synthesized SeNPs	2293:2313	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	12	63	dep	CONCLUSIONS	2276:2286	arg1	NCT/PPE/SeNPs					2352:2364	their innovative nanocomposites NCT/PPE/SeNPs	2320:2364	their innovative nanocomposites NCT/PPE/SeNPs	2320:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	12	63	dep	CONCLUSIONS	2276:2286	arg1	CONCLUSIONS					2276:2286	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	10	64	theme	mycelia	1946:1952	arg1	treatment					1926:1934	The direct treatment	1915:1934	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite	1915:1985	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	1	65	theme	Penicillium	277:287	arg1	digitatum					289:297	Penicillium digitatum	277:297	Penicillium digitatum	277:297	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	7	66	theme	NCT/PPE/SeNPs	1559:1571	arg1	nanocomposites					1573:1586	NCT/PPE/SeNPs nanocomposites	1559:1586	NCT/PPE/SeNPs nanocomposites	1559:1586	The nanoparticles had 82.72, 9.41 and 85.17 nm mean diameters for NCT, PPE/SeNPs and NCT/PPE/SeNPs nanocomposites, respectively.
35392922	2	67	theme	fungicidal	607:616	arg1	extract					446:452	plant extract	440:452	plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	440:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	67	theme	fungicidal	607:616	arg1	agents/nanocomposites					618:638	efficacious fungicidal agents/nanocomposites	595:638	efficacious fungicidal agents/nanocomposites	595:638	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	12	68	theme	PPE-synthesized	2293:2307	arg1	SeNPs					2309:2313	PPE-synthesized SeNPs	2293:2313	PPE-synthesized SeNPs	2293:2313	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	12	68	theme	PPE-synthesized	2293:2307	arg1	CONCLUSIONS					2276:2286	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	6	69	theme	molecular	1332:1340	arg1	bonding					1342:1348	the molecular bonding	1328:1348	the molecular bonding in synthesized agents/composites	1328:1381	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	2	70	dep	P.	651:652	arg1	digitatum					654:662	digitatum	654:662	digitatum	654:662	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	71	theme	P.	651:652	arg1	strains					664:670	P. digitatum strains	651:670	P. digitatum strains	651:670	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	7	72	theme	mean	1521:1524	arg1	diameters					1526:1534	82.72, 9.41 and 85.17 nm mean diameters	1496:1534	diameters	1526:1534	The nanoparticles had 82.72, 9.41 and 85.17 nm mean diameters for NCT, PPE/SeNPs and NCT/PPE/SeNPs nanocomposites, respectively.
35392922	3	73	theme	ionic	796:800	arg1	method					811:816	ionic gelation method	796:816	ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells	796:887	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	0	74	theme	mold	140:143	arg1	protection					145:154	citrus green mold protection	127:154	citrus green mold protection	127:154	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	10	75	theme	NCT/PPE/SeNPs	1959:1971	arg1	nanocomposite					1973:1985	NCT/PPE/SeNPs nanocomposite	1959:1985	NCT/PPE/SeNPs nanocomposite	1959:1985	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	11	76	theme	based	2237:2241	arg1	solutions					2251:2259	based coating solutions	2237:2259	based coating solutions	2237:2259	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	6	77	dep	RESULTS	1246:1252	arg1	achieved					1318:1325	achieved	1318:1325	achieved	1318:1325	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	11	78	theme	mold	2157:2160	arg1	signs					2172:2176	the green mold infection signs	2147:2176	the green mold infection signs	2147:2176	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	1	79	theme	decays	231:236	arg1	worldwide					238:246	fungal decays worldwide	224:246	fungal decays worldwide	224:246	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	9	80	theme	standard	1895:1902	arg1	fungicide					1904:1912	standard fungicide	1895:1912	standard fungicide	1895:1912	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	3	81	theme	chitosan	821:828	arg1	method					811:816	ionic gelation method	796:816	ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells	796:887	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	10	82	theme	remarkable	1994:2003	arg1	lysis					2005:2009	remarkable lysis	1994:2009	remarkable lysis	1994:2009	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	6	83	theme	infrared	1400:1407	arg1	spectroscopy					1409:1420	infrared spectroscopy	1400:1420	infrared spectroscopy	1400:1420	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	11	84	theme	91.7	2181:2184	arg1	%					2199:2199	91.7, 95.4 and 100%	2181:2199	%	2199:2199	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	4	85	theme	generated	957:965	arg1	molecules					967:975	generated molecules	957:975	generated molecules	957:975	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	3	86	theme	prawn	851:855	arg1	shells					882:887	white prawn (Fenneropenaeus indicus) shells	845:887	white prawn (Fenneropenaeus indicus) shells	845:887	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	1	87	theme	BACKGROUND	157:166	arg1	production					175:184	BACKGROUND Citrus production	157:184	BACKGROUND Citrus production	157:184	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	4	88	theme	size	1033:1036	arg1	imaging					1090:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	2	89	from	peels	472:476	arg1	extract					446:452	plant extract	440:452	plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	440:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	89	from	peels	472:476	arg1	agents/nanocomposites					618:638	efficacious fungicidal agents/nanocomposites	595:638	efficacious fungicidal agents/nanocomposites	595:638	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	6	90	theme	PPE-synthesized	1271:1285	arg1	SeNPs					1287:1291	PPE-synthesized SeNPs	1271:1291	PPE-synthesized SeNPs	1271:1291	RESULTS The synthesis of PPE-synthesized SeNPs and NCT was successfully achieved, the molecular bonding in synthesized agents/composites were proved with infrared spectroscopy to have both biochemical and physical interactions.
35392922	11	91	dep	NCT	2206:2208	arg1	solutions					2251:2259	based coating solutions	2237:2259	based coating solutions	2237:2259	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	0	92	theme	green	134:138	arg1	protection					145:154	citrus green mold protection	127:154	citrus green mold protection	127:154	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	8	93	theme	spherical	1636:1644	arg1	shapes					1646:1651	homogenous spherical shapes	1625:1651	homogenous spherical shapes	1625:1651	The nanoparticles had homogenous spherical shapes and good distribution attributes.
35392922	5	94	theme	NCT	1205:1207	arg1	coating					1237:1243	NCT nanocomposites-based edible coating	1205:1243	NCT nanocomposites-based edible coating	1205:1243	Antifungal potentialities were investigated in vitro and in infected fruits with P. digitatum by applying NCT nanocomposites-based edible coating.
35392922	2	95	with	nanoparticles	509:521	arg1	NCT					572:574	NCT	572:574	NCT	572:574	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	95	with	nanoparticles	509:521	arg1	nanoparticles					557:569	PPE (PPE/SeNPs) and chitosan nanoparticles	528:569	PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	528:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	4	96	theme	spectroscopy	1008:1019	arg1	imaging					1090:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	12	97	theme	fungal	2477:2482	arg1	pathogen					2484:2491	postharvest fungal pathogen	2465:2491	postharvest fungal pathogen	2465:2491	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	12	98	theme	edible	2436:2441	arg1	coatings					2443:2450	effectual antifungal and edible coatings	2411:2450	effectual antifungal and edible coatings	2411:2450	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	3	99	theme	Fenneropenaeus	858:871	arg1	shells					882:887	white prawn (Fenneropenaeus indicus) shells	845:887	white prawn (Fenneropenaeus indicus) shells	845:887	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	0	100	theme	citrus	127:132	arg1	protection					145:154	citrus green mold protection	127:154	citrus green mold protection	127:154	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	8	101	theme	distribution	1662:1673	arg1	attributes					1675:1684	good distribution attributes	1657:1684	good distribution attributes	1657:1684	The nanoparticles had homogenous spherical shapes and good distribution attributes.
35392922	2	102	theme	global	424:429	arg1	demands					431:437	global demands	424:437	global demands	424:437	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	102	theme	global	424:429	arg1	substitutions					333:345	The substitutions	329:345	The substitutions of chemical and synthetic fungicides with effectual natural alternatives	329:418	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	4	103	theme	electron	1069:1076	arg1	microscopes					1078:1088	charge assessment and electron microscopes	1047:1088	microscopes	1078:1088	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	11	104	theme	infected	2092:2099	arg1	orange					2101:2106	infected orange	2092:2106	infected orange	2092:2106	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	0	105	theme	biosynthesized	26:39	arg1	selenium					41:48	biosynthesized selenium	26:48	biosynthesized selenium	26:48	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	9	106	theme	potent	1730:1735	arg1	potentialities					1748:1761	potent fungicidal potentialities	1730:1761	potent fungicidal potentialities	1730:1761	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	2	107	theme	pomegranates	459:470	arg1	nanoparticles					509:521	biosynthesized selenium nanoparticles	485:521	biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	485:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	107	theme	pomegranates	459:470	arg1	PPE					479:481	PPE	479:481	PPE	479:481	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	107	theme	pomegranates	459:470	arg1	peels					472:476	pomegranates peels	459:476	pomegranates peels (PPE)	459:482	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	12	108	theme	antifungal	2421:2430	arg1	coatings					2443:2450	effectual antifungal and edible coatings	2411:2450	effectual antifungal and edible coatings	2411:2450	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	10	109	theme	direct	1919:1924	arg1	treatment					1926:1934	The direct treatment	1915:1934	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite	1915:1985	The direct treatment of fungal mycelia with NCT/PPE/SeNPs nanocomposite led to remarkable lysis and deformations of P. digitatum hyphae within 12 h of treatment.
35392922	2	110	theme	biosynthesized	485:498	arg1	nanoparticles					509:521	biosynthesized selenium nanoparticles	485:521	biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT)	485:575	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	2	110	theme	biosynthesized	485:498	arg1	peels					472:476	pomegranates peels	459:476	pomegranates peels (PPE)	459:482	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	3	111	theme	METHOD	673:678	arg1	PPE					680:682	METHOD PPE	673:682	METHOD PPE from Punica granatum	673:703	METHOD PPE from Punica granatum was extracted and employed directly for synthesizing SeNPs, whereas NCT was produced using ionic gelation method of chitosan extracted from white prawn (Fenneropenaeus indicus) shells.
35392922	11	112	theme	edible	2123:2128	arg1	coatings					2130:2137	NCT-based edible coatings	2113:2137	NCT-based edible coatings	2113:2137	The coating of infected orange with NCT-based edible coatings reduced the green mold infection signs by 91.7, 95.4 and 100%, for NCT, NCT/PPE and NCT/PPE/SeNPs based coating solutions, respectively.
35392922	0	113	theme	pomegranate	69:79	arg1	peels					81:85	pomegranate peels	69:85	pomegranate peels	69:85	Antifungal application of biosynthesized selenium nanoparticles with pomegranate peels and nanochitosan as edible coatings for citrus green mold protection.
35392922	5	114	theme	edible	1230:1235	arg1	coating					1237:1243	NCT nanocomposites-based edible coating	1205:1243	NCT nanocomposites-based edible coating	1205:1243	Antifungal potentialities were investigated in vitro and in infected fruits with P. digitatum by applying NCT nanocomposites-based edible coating.
35392922	9	115	theme	P.	1770:1771	arg1	isolates					1783:1790	P. digitatum isolates	1770:1790	P. digitatum isolates	1770:1790	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	4	116	theme	assessment	1054:1063	arg1	imaging					1090:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging	998:1096	The physiochemical, biochemical and structural characterization of generated molecules were conducted using infra-red spectroscopy, particles' size (Ps) and charge assessment and electron microscopes imaging.
35392922	2	117	with	fungicides	373:382	arg1	alternatives					407:418	effectual natural alternatives	389:418	effectual natural alternatives	389:418	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	9	118	theme	NCT/PPE/SeNPs	1793:1805	arg1	forceful					1834:1841	forceful	1834:1841	forceful	1834:1841	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	9	118	theme	NCT/PPE/SeNPs	1793:1805	arg1	nanocomposite					1807:1819	NCT/PPE/SeNPs nanocomposite	1793:1819	NCT/PPE/SeNPs nanocomposite	1793:1819	The entire agents/nanocomposites exhibited potent fungicidal potentialities toward P. digitatum isolates; NCT/PPE/SeNPs nanocomposite was the most forceful and significantly exceeded the fungicidal action of standard fungicide.
35392922	12	119	theme	nanocomposites	2337:2350	arg1	NCT/PPE/SeNPs					2352:2364	their innovative nanocomposites NCT/PPE/SeNPs	2320:2364	their innovative nanocomposites NCT/PPE/SeNPs	2320:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	12	119	theme	nanocomposites	2337:2350	arg1	CONCLUSIONS					2276:2286	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs	2276:2364	CONCLUSIONS NCT, PPE-synthesized SeNPs, and their innovative nanocomposites NCT/PPE/SeNPs are convincingly recommended for formulating effectual antifungal and edible coatings to eliminate postharvest fungal pathogen, both with protection from their invasion or with destructing their existing infections.
35392922	2	120	theme	effectual	389:397	arg1	alternatives					407:418	effectual natural alternatives	389:418	effectual natural alternatives	389:418	The substitutions of chemical and synthetic fungicides with effectual natural alternatives are global demands; plant extract from pomegranates peels (PPE), biosynthesized selenium nanoparticles with PPE (PPE/SeNPs) and chitosan nanoparticles (NCT) were suggested as efficacious fungicidal agents/nanocomposites to control P. digitatum strains.
35392922	1	121	theme	mold	259:262	arg1	disastrous					317:326	disastrous	317:326	disastrous	317:326	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
35392922	1	121	theme	mold	259:262	arg1	infection					264:272	the green mold infection	249:272	the green mold infection by Penicillium digitatum	249:297	BACKGROUND Citrus production and trading are seriously affected by fungal decays worldwide; the green mold infection by Penicillium digitatum could be the most disastrous.
36678325	0	0	theme	Dietary	72:78	arg1	Fiber					80:84	Dietary Fiber	72:84	Dietary Fiber	72:84	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	2	1	theme	diet	470:473	arg1	effects					445:451	metabolic effects	435:451	metabolic effects of an obesogenic diet	435:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	7	2	theme	gut	1130:1132	arg1	composition					1145:1155	gut microbiota composition	1130:1155	gut microbiota composition	1130:1155	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	1	3	theme	microbiota	145:154	arg1	tool					192:195	a therapeutic tool	178:195	a therapeutic tool for treating obesity and related disorders	178:238	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	1	3	theme	microbiota	145:154	arg1	transfer					156:163	Fecal microbiota transfer	139:163	Fecal microbiota transfer	139:163	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	2	4	theme	obesogenic	459:468	arg1	diet					470:473	an obesogenic diet	456:473	an obesogenic diet	456:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	0	5	from	Effect	11:16	arg1	Steatosis					47:55	Liver Steatosis	41:55	Liver Steatosis	41:55	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	6	6	from	changes	991:997	arg1	mice					1029:1032	non-humanized conventional mice	1002:1032	non-humanized conventional mice	1002:1032	In contrast, inulin did not affect WD-induced metabolic changes in non-humanized conventional mice.
36678325	7	7	theme	sulfur-containing	1272:1288	arg1	compounds					1290:1298	sulfur-containing compounds	1272:1298	sulfur-containing compounds	1272:1298	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	8	8	theme	adverse	1386:1392	arg1	effects					1394:1400	adverse effects	1386:1400	adverse effects of WD	1386:1406	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	2	9	theme	potential	483:491	arg1	role					493:496	the potential role	479:496	the potential role of dietary inulin in mediating these effects	479:541	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	10	theme	dietary	501:507	arg1	inulin					509:514	dietary inulin	501:514	dietary inulin	501:514	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	11	from	impact	425:430	arg1	effects					445:451	metabolic effects	435:451	metabolic effects of an obesogenic diet	435:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	5	12	with	supplementation	854:868	arg1	inulin					875:880	inulin	875:880	inulin	875:880	However, supplementation with inulin reversed steatosis and improved glucose homeostasis.
36678325	9	13	from	shift	1539:1543	arg1	expense					1625:1631	the expense	1621:1631	the expense of proteolytic fermentation	1621:1659	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	9	13	from	shift	1539:1543	arg1	composition					1559:1569	microbiota composition	1548:1569	microbiota composition	1548:1569	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	9	13	from	shift	1539:1543	arg1	accentuation					1575:1586	accentuation	1575:1586	accentuation of saccharolytic fermentation at the expense of proteolytic fermentation	1575:1659	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	7	14	theme	saccharolytic	1232:1244	arg1	fermentation					1246:1257	saccharolytic fermentation	1232:1257	saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA)	1232:1317	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	4	15	theme	vegan	723:727	arg1	microbiota					729:738	vegan microbiota	723:738	vegan microbiota	723:738	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	6	16	theme	WD-induced	970:979	arg1	changes					991:997	WD-induced metabolic changes	970:997	WD-induced metabolic changes in non-humanized conventional mice	970:1032	In contrast, inulin did not affect WD-induced metabolic changes in non-humanized conventional mice.
36678325	9	17	theme	fermentation	1605:1616	arg1	composition					1559:1569	microbiota composition	1548:1569	microbiota composition	1548:1569	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	9	17	theme	fermentation	1605:1616	arg1	accentuation					1575:1586	accentuation	1575:1586	accentuation of saccharolytic fermentation at the expense of proteolytic fermentation	1575:1659	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	9	18	theme	microbiota	1548:1557	arg1	composition					1559:1569	microbiota composition	1548:1569	microbiota composition	1548:1569	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	8	19	theme	vegan	1338:1342	arg1	microbiota					1344:1353	vegan microbiota	1338:1353	vegan microbiota	1338:1353	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	5	20	theme	glucose	914:920	arg1	homeostasis					922:932	glucose homeostasis	914:932	glucose homeostasis	914:932	However, supplementation with inulin reversed steatosis and improved glucose homeostasis.
36678325	8	21	dep	supplementation	1418:1432	arg1	ii					1414:1415	ii	1414:1415	ii	1414:1415	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	4	22	theme	excessive	752:760	arg1	gain					769:772	excessive weight gain	752:772	excessive weight gain	752:772	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	0	23	theme	Liver	41:45	arg1	Steatosis					47:55	Liver Steatosis	41:55	Liver Steatosis	41:55	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	7	24	theme	significant	1108:1118	arg1	change					1120:1125	a significant change	1106:1125	a significant change in gut microbiota composition and its metabolic performance	1106:1185	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	4	25	theme	liver	828:832	arg1	steatosis					834:842	liver steatosis	828:842	liver steatosis	828:842	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	7	26	dep	fermentation	1246:1257	arg1	increase					1301:1308	increase	1301:1308	increase of SCFA	1301:1316	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	7	26	dep	fermentation	1246:1257	arg1	decrease					1260:1267	decrease	1260:1267	decrease of sulfur-containing compounds	1260:1298	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	3	27	theme	Ex-germ-free	544:555	arg1	animals					557:563	Ex-germ-free animals	544:563	Ex-germ-free animals	544:563	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	7	28	theme	microbiota-colonized	1044:1063	arg1	mice					1065:1068	vegan microbiota-colonized mice	1038:1068	vegan microbiota-colonized mice	1038:1068	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	0	29	theme	Protective	0:9	arg1	Effect					11:16	Protective Effect	0:16	Protective Effect of Vegan Microbiota on Liver Steatosis	0:55	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	7	30	theme	metabolic	1165:1173	arg1	performance					1175:1185	its metabolic performance	1161:1185	its metabolic performance	1161:1185	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	9	31	theme	fermentation	1648:1659	arg1	expense					1625:1631	the expense	1621:1631	the expense of proteolytic fermentation	1621:1659	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	2	32	from	donors	359:364	arg1	microbiota					337:346	microbiota	337:346	microbiota	337:346	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	33	from	role	493:496	arg1	effects					445:451	metabolic effects	435:451	metabolic effects of an obesogenic diet	435:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	9	34	from	expense	1625:1631	arg1	composition					1559:1569	microbiota composition	1548:1569	microbiota composition	1548:1569	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	9	34	from	expense	1625:1631	arg1	accentuation					1575:1586	accentuation	1575:1586	accentuation of saccharolytic fermentation at the expense of proteolytic fermentation	1575:1659	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	9	34	from	expense	1625:1631	arg1	shift					1539:1543	the shift	1535:1543	the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation	1535:1659	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	7	35	theme	microbiota	1134:1143	arg1	composition					1145:1155	gut microbiota composition	1130:1155	gut microbiota composition	1130:1155	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	0	36	theme	Vegan	21:25	arg1	Microbiota					27:36	Vegan Microbiota	21:36	Vegan Microbiota	21:36	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	4	37	theme	impaired	775:782	arg1	metabolism					792:801	impaired glucose metabolism	775:801	impaired glucose metabolism	775:801	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	0	38	theme	Microbiota	110:119	arg1	Therapy					130:136	Fecal Microbiota Transfer Therapy	104:136	Fecal Microbiota Transfer Therapy	104:136	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	9	39	theme	saccharolytic	1591:1603	arg1	fermentation					1605:1616	saccharolytic fermentation	1591:1616	saccharolytic fermentation	1591:1616	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	2	40	theme	non-communicable	398:413	arg1	diseases					415:422	non-communicable diseases	398:422	non-communicable diseases	398:422	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	41	theme	diseases	415:422	arg1	impact					425:430	impact	425:430	impact on metabolic effects of an obesogenic diet	425:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	41	theme	diseases	415:422	arg1	incidence					385:393	a low incidence	379:393	a low incidence of non-communicable diseases	379:422	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	41	theme	diseases	415:422	arg1	role					493:496	the potential role	479:496	the potential role of dietary inulin in mediating these effects	479:541	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	0	42	theme	Fecal	104:108	arg1	Therapy					130:136	Fecal Microbiota Transfer Therapy	104:136	Fecal Microbiota Transfer Therapy	104:136	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	2	43	dep	studied	325:331	arg1	microbiota					337:346	microbiota	337:346	microbiota	337:346	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	3	44	theme	Western-type	630:641	arg1	WD					649:650	WD	649:650	WD	649:650	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	3	44	theme	Western-type	630:641	arg1	diet					643:646	a standard or Western-type diet	616:646	a standard or Western-type diet (WD)	616:651	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	8	45	theme	WD	1405:1406	arg1	effects					1394:1400	adverse effects	1386:1400	adverse effects of WD	1386:1406	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	8	46	dep	protect	1370:1376	arg1	i					1335:1335	i	1335:1335	i	1335:1335	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	8	47	with	supplementation	1418:1432	arg1	inulin					1439:1444	inulin	1439:1444	inulin	1439:1444	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	0	48	theme	Microbiota	27:36	arg1	Effect					11:16	Protective Effect	0:16	Protective Effect of Vegan Microbiota on Liver Steatosis	0:55	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	7	49	from	change	1120:1125	arg1	composition					1145:1155	gut microbiota composition	1130:1155	gut microbiota composition	1130:1155	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	7	49	from	change	1120:1125	arg1	performance					1175:1185	its metabolic performance	1161:1185	its metabolic performance	1161:1185	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	0	50	theme	Transfer	121:128	arg1	Therapy					130:136	Fecal Microbiota Transfer Therapy	104:136	Fecal Microbiota Transfer Therapy	104:136	Protective Effect of Vegan Microbiota on Liver Steatosis Is Conveyed by Dietary Fiber: Implications for Fecal Microbiota Transfer Therapy.
36678325	6	51	theme	metabolic	981:989	arg1	changes					991:997	WD-induced metabolic changes	970:997	WD-induced metabolic changes in non-humanized conventional mice	970:1032	In contrast, inulin did not affect WD-induced metabolic changes in non-humanized conventional mice.
36678325	3	52	theme	human	585:589	arg1	microbiota					597:606	human vegan microbiota	585:606	human vegan microbiota	585:606	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	2	53	theme	low	381:383	arg1	incidence					385:393	a low incidence	379:393	a low incidence of non-communicable diseases	379:422	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	8	54	theme	normalized	1469:1478	arg1	metabolism					1488:1497	normalized glucose metabolism	1469:1497	normalized glucose metabolism	1469:1497	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	1	55	theme	optimal	291:297	arg1	characteristics					305:319	the optimal donor characteristics	287:319	the optimal donor characteristics	287:319	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	7	56	theme	vegan	1038:1042	arg1	mice					1065:1068	vegan microbiota-colonized mice	1038:1068	vegan microbiota-colonized mice	1038:1068	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	3	57	theme	standard	618:625	arg1	WD					649:650	WD	649:650	WD	649:650	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	3	57	theme	standard	618:625	arg1	diet					643:646	a standard or Western-type diet	616:646	a standard or Western-type diet (WD)	616:651	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	4	58	theme	insulin	804:810	arg1	resistance					812:821	insulin resistance	804:821	insulin resistance	804:821	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	7	59	theme	SCFA	1313:1316	arg1	increase					1301:1308	increase	1301:1308	increase of SCFA	1301:1316	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	7	59	theme	SCFA	1313:1316	arg1	decrease					1260:1267	decrease	1260:1267	decrease of sulfur-containing compounds	1260:1298	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	2	60	theme	inulin	509:514	arg1	impact					425:430	impact	425:430	impact on metabolic effects of an obesogenic diet	425:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	60	theme	inulin	509:514	arg1	incidence					385:393	a low incidence	379:393	a low incidence of non-communicable diseases	379:422	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	2	60	theme	inulin	509:514	arg1	role					493:496	the potential role	479:496	the potential role of dietary inulin in mediating these effects	479:541	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	1	61	theme	donor	299:303	arg1	characteristics					305:319	the optimal donor characteristics	287:319	the optimal donor characteristics	287:319	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	7	62	theme	inulin	1071:1076	arg1	supplementation					1078:1092	inulin supplementation	1071:1092	inulin supplementation	1071:1092	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	2	63	theme	metabolic	435:443	arg1	effects					445:451	metabolic effects	435:451	metabolic effects of an obesogenic diet	435:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	7	64	from	proteolytic	1212:1222	arg1	shift					1201:1205	the shift	1197:1205	the shift from proteolytic	1197:1222	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	6	65	theme	non-humanized	1002:1014	arg1	mice					1029:1032	non-humanized conventional mice	1002:1032	non-humanized conventional mice	1002:1032	In contrast, inulin did not affect WD-induced metabolic changes in non-humanized conventional mice.
36678325	4	66	with	colonization	705:716	arg1	microbiota					729:738	vegan microbiota	723:738	vegan microbiota	723:738	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	9	67	theme	proteolytic	1636:1646	arg1	fermentation					1648:1659	proteolytic fermentation	1636:1659	proteolytic fermentation	1636:1659	This phenomenon is associated with the shift in microbiota composition and accentuation of saccharolytic fermentation at the expense of proteolytic fermentation.
36678325	4	68	theme	glucose	784:790	arg1	metabolism					792:801	impaired glucose metabolism	775:801	impaired glucose metabolism	775:801	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	4	69	theme	weight	762:767	arg1	gain					769:772	excessive weight gain	752:772	excessive weight gain	752:772	Despite the colonization with vegan microbiota, WD induced excessive weight gain, impaired glucose metabolism, insulin resistance, and liver steatosis.
36678325	2	70	from	incidence	385:393	arg1	effects					445:451	metabolic effects	435:451	metabolic effects of an obesogenic diet	435:473	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36678325	1	71	theme	Fecal	139:143	arg1	tool					192:195	a therapeutic tool	178:195	a therapeutic tool for treating obesity and related disorders	178:238	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	1	71	theme	Fecal	139:143	arg1	transfer					156:163	Fecal microbiota transfer	139:163	Fecal microbiota transfer	139:163	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	6	72	theme	conventional	1016:1027	arg1	mice					1029:1032	non-humanized conventional mice	1002:1032	non-humanized conventional mice	1002:1032	In contrast, inulin did not affect WD-induced metabolic changes in non-humanized conventional mice.
36678325	8	73	theme	glucose	1480:1486	arg1	metabolism					1488:1497	normalized glucose metabolism	1469:1497	normalized glucose metabolism	1469:1497	We found that (i) vegan microbiota alone does not protect against adverse effects of WD; and (ii) supplementation with inulin reversed steatosis and normalized glucose metabolism.
36678325	3	74	theme	vegan	591:595	arg1	microbiota					597:606	human vegan microbiota	585:606	human vegan microbiota	585:606	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	1	75	theme	therapeutic	180:190	arg1	tool					192:195	a therapeutic tool	178:195	a therapeutic tool for treating obesity and related disorders	178:238	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	1	75	theme	therapeutic	180:190	arg1	transfer					156:163	Fecal microbiota transfer	139:163	Fecal microbiota transfer	139:163	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	7	76	theme	compounds	1290:1298	arg1	increase					1301:1308	increase	1301:1308	increase of SCFA	1301:1316	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	7	76	theme	compounds	1290:1298	arg1	decrease					1260:1267	decrease	1260:1267	decrease of sulfur-containing compounds	1260:1298	In vegan microbiota-colonized mice, inulin supplementation resulted in a significant change in gut microbiota composition and its metabolic performance, inducing the shift from proteolytic towards saccharolytic fermentation (decrease of sulfur-containing compounds, increase of SCFA).
36678325	1	77	theme	related	222:228	arg1	disorders					230:238	related disorders	222:238	related disorders	222:238	Fecal microbiota transfer may serve as a therapeutic tool for treating obesity and related disorders but currently, there is no consensus regarding the optimal donor characteristics.
36678325	3	78	theme	inulin	669:674	arg1	supplementation					676:690	inulin supplementation	669:690	inulin supplementation	669:690	Ex-germ-free animals were colonized with human vegan microbiota and fed a standard or Western-type diet (WD) with or without inulin supplementation.
36678325	2	79	theme	vegan	353:357	arg1	donors					359:364	vegan donors	353:364	vegan donors	353:364	We studied how microbiota from vegan donors, who exhibit a low incidence of non-communicable diseases, impact on metabolic effects of an obesogenic diet and the potential role of dietary inulin in mediating these effects.
36521212	0	0	theme	semi-continuous	73:87	arg1	investigation					95:107	semi-continuous scale investigation	73:107	semi-continuous scale investigation	73:107	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.
36521212	4	1	theme	potential	643:651	arg1	production					661:670	the potential methane production	639:670	the potential methane production from each substrate at batch scale	639:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	1	2	theme	bioplastics	147:157	arg1	behaviour					134:142	the behaviour	130:142	the behaviour of bioplastics in anaerobic co-digestion	130:183	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	5	3	theme	methane	856:862	arg1	yield					864:868	a methane yield	854:868	a methane yield of 331 NmlCH4/gVS	854:886	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	7	4	theme	specimens	1303:1311	arg1	accumulation					1284:1295	the accumulation	1280:1295	the accumulation of CA specimens	1280:1311	An increase was registered after 65 days of semi-continuous process, due to the accumulation of CA specimens.
36521212	4	5	from	evaluation	625:634	arg1	scale					701:705	each substrate at batch scale	677:705	each substrate at batch scale	677:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	1	6	theme	batch	248:252	arg1	mode					254:257	batch mode	248:257	batch mode	248:257	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	0	7	theme	scale	89:93	arg1	investigation					95:107	semi-continuous scale investigation	73:107	semi-continuous scale investigation	73:107	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.
36521212	6	8	theme	production	1118:1127	arg1	rates					1129:1133	methane production rates	1110:1133	methane production rates	1110:1133	The results were both lower than the one obtained from batch co-digestion, although methane production rates were comparable regardless of being fed with or without bioplastics.
36521212	1	9	from	behaviour	134:142	arg1	co-digestion					172:183	anaerobic co-digestion	162:183	anaerobic co-digestion	162:183	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	3	10	theme	food	502:505	arg1	waste					507:511	food waste	502:511	food waste	502:511	This work aims to cover this gap, carrying out a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored).
36521212	8	11	dep	bioplastics	1369:1379	arg1	waste					1390:1394	waste	1390:1394	waste	1390:1394	This confirms the different degradation trends between bioplastics and food waste.
36521212	4	12	theme	production	661:670	arg1	evaluation					625:634	a first evaluation	617:634	a first evaluation of the potential methane production from each substrate at batch scale	617:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	4	13	theme	at	692:693	arg1	scale					701:705	each substrate at batch scale	677:705	each substrate at batch scale	677:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	3	14	theme	anaerobic	476:484	arg1	co-digestion					486:497	a semi-continuous anaerobic co-digestion	458:497	a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored)	458:608	This work aims to cover this gap, carrying out a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored).
36521212	4	15	theme	first	619:623	arg1	evaluation					625:634	a first evaluation	617:634	a first evaluation of the potential methane production from each substrate at batch scale	617:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	1	16	theme	anaerobic	162:170	arg1	co-digestion					172:183	anaerobic co-digestion	162:183	anaerobic co-digestion	162:183	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	4	17	from	scale	701:705	arg1	batch					695:699	each substrate at batch scale	677:705	each substrate at batch scale	677:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	4	17	from	scale	701:705	arg1	production					661:670	the potential methane production	639:670	the potential methane production from each substrate at batch scale	639:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	4	17	from	scale	701:705	arg1	evaluation					625:634	a first evaluation	617:634	a first evaluation of the potential methane production from each substrate at batch scale	617:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	4	17	from	scale	701:705	arg1	at					692:693	each substrate at batch scale	677:705	each substrate at batch scale	677:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	4	18	theme	semi-continuous	712:726	arg1	co-digestion					728:739	the semi-continuous co-digestion	708:739	the semi-continuous co-digestion of food waste and cellulose acetate	708:775	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	8	19	theme	degradation	1342:1352	arg1	trends					1354:1359	the different degradation trends	1328:1359	the different degradation trends between bioplastics and food waste	1328:1394	This confirms the different degradation trends between bioplastics and food waste.
36521212	3	20	theme	cellulose	517:525	arg1	acetate					527:533	cellulose acetate	517:533	cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored)	517:608	This work aims to cover this gap, carrying out a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored).
36521212	2	21	theme	batch	362:366	arg1	ones					368:371	batch ones	362:371	batch ones	362:371	Despite the fact that continuous experiments confirm or add new insight to the findings acquired from batch ones, there is still lack of such studies.
36521212	2	22	theme	studies	402:408	arg1	lack					389:392	lack	389:392	lack of such studies	389:408	Despite the fact that continuous experiments confirm or add new insight to the findings acquired from batch ones, there is still lack of such studies.
36521212	3	23	theme	acetate	527:533	arg1	co-digestion					486:497	a semi-continuous anaerobic co-digestion	458:497	a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored)	458:608	This work aims to cover this gap, carrying out a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored).
36521212	5	24	theme	semi-continuous	829:843	arg1	process					845:851	the semi-continuous process	825:851	the semi-continuous process	825:851	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	2	25	theme	such	397:400	arg1	studies					402:408	such studies	397:408	such studies	397:408	Despite the fact that continuous experiments confirm or add new insight to the findings acquired from batch ones, there is still lack of such studies.
36521212	4	26	theme	substrate	682:690	arg1	scale					701:705	each substrate at batch scale	677:705	each substrate at batch scale	677:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	1	27	from	studies	119:125	arg1	behaviour					134:142	the behaviour	130:142	the behaviour of bioplastics in anaerobic co-digestion	130:183	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	3	28	theme	waste	507:511	arg1	co-digestion					486:497	a semi-continuous anaerobic co-digestion	458:497	a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored)	458:608	This work aims to cover this gap, carrying out a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored).
36521212	5	29	theme	bioplastics	966:976	arg1	specimens					978:986	bioplastics specimens	966:986	bioplastics specimens	966:986	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	7	30	theme	semi-continuous	1248:1262	arg1	process					1264:1270	semi-continuous process	1248:1270	semi-continuous process	1248:1270	An increase was registered after 65 days of semi-continuous process, due to the accumulation of CA specimens.
36521212	0	31	theme	food	16:19	arg1	waste					21:25	food waste	16:25	food waste	16:25	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.
36521212	5	32	theme	food	927:930	arg1	waste					932:936	food waste	927:936	food waste	927:936	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	8	33	theme	different	1332:1340	arg1	trends					1354:1359	the different degradation trends	1328:1359	the different degradation trends between bioplastics and food waste	1328:1394	This confirms the different degradation trends between bioplastics and food waste.
36521212	4	34	theme	batch	695:699	arg1	scale					701:705	each substrate at batch scale	677:705	each substrate at batch scale	677:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	7	35	theme	process	1264:1270	arg1	65 days					1237:1243	65 days	1237:1243	65 days of semi-continuous process	1237:1270	An increase was registered after 65 days of semi-continuous process, due to the accumulation of CA specimens.
36521212	0	36	theme	cellulose-based	31:45	arg1	bioplastic					47:56	cellulose-based bioplastic	31:56	cellulose-based bioplastic	31:56	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.
36521212	4	37	theme	methane	653:659	arg1	production					661:670	the potential methane production	639:670	the potential methane production from each substrate at batch scale	639:705	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	5	38	theme	%	1023:1023	arg1	loss					1006:1009	a weight loss	997:1009	a weight loss of about 45 %	997:1023	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	4	39	theme	acetate	769:775	arg1	co-digestion					728:739	the semi-continuous co-digestion	708:739	the semi-continuous co-digestion of food waste and cellulose acetate	708:775	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	0	40	from	batch	64:68	arg1	Co-digestion					0:11	Co-digestion	0:11	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.	0:108	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.
36521212	0	41	theme	waste	21:25	arg1	Co-digestion					0:11	Co-digestion	0:11	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.	0:108	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.
36521212	1	42	theme	few	115:117	arg1	studies					119:125	Only few studies	110:125	Only few studies on the behaviour of bioplastics in anaerobic co-digestion	110:183	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	0	43	theme	bioplastic	47:56	arg1	Co-digestion					0:11	Co-digestion	0:11	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.	0:108	Co-digestion of food waste and cellulose-based bioplastic: From batch to semi-continuous scale investigation.
36521212	5	44	theme	weight	999:1004	arg1	loss					1006:1009	a weight loss	997:1009	a weight loss of about 45 %	997:1023	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	6	45	theme	batch	1081:1085	arg1	co-digestion					1087:1098	batch co-digestion	1081:1098	batch co-digestion	1081:1098	The results were both lower than the one obtained from batch co-digestion, although methane production rates were comparable regardless of being fed with or without bioplastics.
36521212	2	46	theme	new	320:322	arg1	insight					324:330	new insight	320:330	new insight	320:330	Despite the fact that continuous experiments confirm or add new insight to the findings acquired from batch ones, there is still lack of such studies.
36521212	4	47	theme	waste	749:753	arg1	co-digestion					728:739	the semi-continuous co-digestion	708:739	the semi-continuous co-digestion of food waste and cellulose acetate	708:775	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	1	48	located	found	194:198	arg1	literature					203:212	literature	203:212	literature	203:212	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	1	48	located	found	194:198	arg2	studies					119:125	Only few studies	110:125	Only few studies on the behaviour of bioplastics in anaerobic co-digestion	110:183	Only few studies on the behaviour of bioplastics in anaerobic co-digestion could be found in literature and most of them are conducted in batch mode.
36521212	4	49	theme	food	744:747	arg1	waste					749:753	food waste	744:753	food waste	744:753	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	4	50	theme	cellulose	759:767	arg1	acetate					769:775	cellulose acetate	759:775	cellulose acetate	759:775	After a first evaluation of the potential methane production from each substrate at batch scale, the semi-continuous co-digestion of food waste and cellulose acetate was carried out in three configurations.
36521212	2	51	theme	continuous	282:291	arg1	experiments					293:303	continuous experiments	282:303	continuous experiments	282:303	Despite the fact that continuous experiments confirm or add new insight to the findings acquired from batch ones, there is still lack of such studies.
36521212	5	52	theme	waste	932:936	arg1	co-digestion					911:922	the co-digestion	907:922	the co-digestion of food waste and cellulose acetate	907:958	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	5	53	theme	cellulose	942:950	arg1	acetate					952:958	cellulose acetate	942:958	cellulose acetate	942:958	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	5	54	theme	NmlCH4/gVS	877:886	arg1	yield					864:868	a methane yield	854:868	a methane yield of 331 NmlCH4/gVS	854:886	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	5	55	theme	acetate	952:958	arg1	co-digestion					911:922	the co-digestion	907:922	the co-digestion of food waste and cellulose acetate	907:958	During the semi-continuous process, a methane yield of 331 NmlCH4/gVS was generated from the co-digestion of food waste and cellulose acetate while bioplastics specimens achieved a weight loss of about 45 %.
36521212	6	56	theme	methane	1110:1116	arg1	rates					1129:1133	methane production rates	1110:1133	methane production rates	1110:1133	The results were both lower than the one obtained from batch co-digestion, although methane production rates were comparable regardless of being fed with or without bioplastics.
36521212	7	57	theme	CA	1300:1301	arg1	specimens					1303:1311	CA specimens	1300:1311	CA specimens	1300:1311	An increase was registered after 65 days of semi-continuous process, due to the accumulation of CA specimens.
36521212	3	58	theme	anaerobic	562:570	arg1	environment					572:582	anaerobic environment	562:582	anaerobic environment	562:582	This work aims to cover this gap, carrying out a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored).
36521212	3	59	theme	semi-continuous	460:474	arg1	co-digestion					486:497	a semi-continuous anaerobic co-digestion	458:497	a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored)	458:608	This work aims to cover this gap, carrying out a semi-continuous anaerobic co-digestion of food waste and cellulose acetate (which its behaviour under anaerobic environment is also quite unexplored).
36532477	13	0	from	h	2134:2134	arg1	<					2121:2121	p < 0.05	2119:2126	p < 0.05 at 24 h	2119:2134	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	14	1	attach	present	2302:2308	arg2	biofilms					2293:2300	S. aureus biofilms	2283:2300	S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies	2283:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	14	1	attach	present	2302:2308	arg1	industry					2323:2330	the dairy industry	2313:2330	the dairy industry	2313:2330	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	2	2	theme	complex	316:322	arg1	system					324:329	a complex system	314:329	a complex system	314:329	Biofilm is a complex system, influenced by nutritional-related factors that regulate the synthesis of the components of the biofilm matrix.
36532477	2	2	theme	complex	316:322	arg1	Biofilm					303:309	Biofilm	303:309	Biofilm	303:309	Biofilm is a complex system, influenced by nutritional-related factors that regulate the synthesis of the components of the biofilm matrix.
36532477	4	3	from	TSBNaCl	670:676	arg1	SS					712:713	SS	712:713	SS	712:713	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	4	3	from	TSBNaCl	670:676	arg1	stainless-steel					695:709	stainless-steel	695:709	stainless-steel (SS)	695:714	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	13	4	with	treatment	1999:2007	arg1	K					2025:2025	proteinase K	2014:2025	proteinase K	2014:2025	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	10	5	theme	Epifluorescence	1444:1458	arg1	microscopy					1460:1469	Epifluorescence microscopy	1444:1469	Epifluorescence microscopy	1444:1469	Epifluorescence microscopy and SEM revealed the metabolically active cells and the different stages of biofilm formation.
36532477	4	6	from	TSBG	664:667	arg1	SS					712:713	SS	712:713	SS	712:713	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	4	6	from	TSBG	664:667	arg1	stainless-steel					695:709	stainless-steel	695:709	stainless-steel (SS)	695:714	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	6	7	theme	FCS-polypropylene	1094:1110	arg1	[n					1112:1113	FCS-polypropylene [n	1094:1113	FCS-polypropylene [n = 110]) for this study	1094:1136	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	6	8	dep	=	1083:1083	arg1	183					1085:1087	183	1085:1087	183	1085:1087	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	13	9	theme	biofilm	2089:2095	arg1	detachment					2063:2072	biomass detachment	2055:2072	biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h)	2055:2135	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	13	9	theme	biofilm	2089:2095	arg1	%					2079:2079	17.7%	2075:2079	17.7%	2075:2079	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	4	10	from	TSB	659:661	arg1	SS					712:713	SS	712:713	SS	712:713	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	4	10	from	TSB	659:661	arg1	stainless-steel					695:709	stainless-steel	695:709	stainless-steel (SS)	695:714	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	14	11	theme	dairy	2317:2321	arg1	industry					2323:2330	the dairy industry	2313:2330	the dairy industry	2313:2330	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	13	12	dep	TSBGNaCl	2172:2179	arg1	%					2202:2202	33.6, 36.9, and 37.8%	2182:2202	33.6, 36.9, and 37.8%	2182:2202	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	6	13	theme	=	1083:1083	arg1	[n					1080:1081	FCS-stainless steel [n	1060:1081	FCS-stainless steel [n = 183	1060:1087	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	14	14	theme	disinfection	2403:2414	arg1	strategies					2416:2425	more efficient composition-specific disinfection strategies	2367:2425	more efficient composition-specific disinfection strategies	2367:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	3	15	theme	growth	554:559	arg1	conditions					561:570	different growth conditions	544:570	different growth conditions	544:570	This study determines the prevalence of biofilm-associated genes and evaluates the development under different growth conditions and compositions of biofilms produced by S. aureus.
36532477	1	16	theme	contact	206:212	arg1	FCS					224:226	FCS	224:226	FCS	224:226	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	1	16	theme	contact	206:212	arg1	surfaces					214:221	food contact surfaces	201:221	food contact surfaces (FCS)	201:227	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	14	17	theme	biofilms	2293:2300	arg1	composition					2268:2278	the composition	2264:2278	the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies	2264:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	14	18	theme	efficient	2372:2380	arg1	strategies					2416:2425	more efficient composition-specific disinfection strategies	2367:2425	more efficient composition-specific disinfection strategies	2367:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	13	19	dep	TSBNaCl	2110:2116	arg1	<					2121:2121	p < 0.05	2119:2126	p < 0.05 at 24 h	2119:2134	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	13	20	theme	biomass	2055:2061	arg1	detachment					2063:2072	biomass detachment	2055:2072	biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h)	2055:2135	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	13	20	theme	biomass	2055:2061	arg1	%					2079:2079	17.7%	2075:2079	17.7%	2075:2079	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	1	21	from	biofilms	189:196	arg1	industry					242:249	the dairy industry	232:249	the dairy industry	232:249	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	1	21	from	biofilms	189:196	arg1	FCS					224:226	FCS	224:226	FCS	224:226	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	1	21	from	biofilms	189:196	arg1	surfaces					214:221	food contact surfaces	201:221	food contact surfaces (FCS)	201:227	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	4	22	from	h	748:748	arg1	enumeration					722:732	enumeration	722:732	enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM)	722:817	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	1	23	theme	important	156:164	arg1	Introduction					115:126	Introduction	115:126	Introduction Staphylococcus aureus	115:148	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	1	23	theme	important	156:164	arg1	pathogen					166:173	an important pathogen	153:173	an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern	153:300	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	11	24	from	biofilms	1626:1633	arg1	SS					1638:1639	SS	1638:1639	SS	1638:1639	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	0	25	theme	food	92:95	arg1	surfaces					105:112	food contact surfaces	92:112	food contact surfaces	92:112	Genetic and compositional analysis of biofilm formed by Staphylococcus aureus isolated from food contact surfaces.
36532477	11	26	theme	aureus	1619:1624	arg1	polymeric					1603:1611	polymeric	1603:1611	polymeric	1603:1611	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	12	27	from	h	1877:1877	arg1	NaCl					1866:1869	NaCl	1866:1869	NaCl at 24 h	1866:1877	Finally, the level of detachment on being treated with DNase I (44.7%) and NaIO 4(42.4%) was greater in the biofilms developed in TSB compared to culture medium supplemented with NaCl at 24 h; however, there was no significant difference when the culture medium was supplemented with glucose.
36532477	12	28	theme	culture	1833:1839	arg1	medium					1841:1846	culture medium	1833:1846	culture medium supplemented with NaCl at 24 h	1833:1877	Finally, the level of detachment on being treated with DNase I (44.7%) and NaIO 4(42.4%) was greater in the biofilms developed in TSB compared to culture medium supplemented with NaCl at 24 h; however, there was no significant difference when the culture medium was supplemented with glucose.
36532477	2	29	theme	nutritional-related	346:364	arg1	factors					366:372	nutritional-related factors	346:372	nutritional-related factors that regulate the synthesis of the components of the biofilm matrix	346:440	Biofilm is a complex system, influenced by nutritional-related factors that regulate the synthesis of the components of the biofilm matrix.
36532477	0	30	theme	Genetic	0:6	arg1	analysis					26:33	Genetic and compositional analysis	0:33	Genetic and compositional analysis of biofilm	0:44	Genetic and compositional analysis of biofilm formed by Staphylococcus aureus isolated from food contact surfaces.
36532477	5	31	theme	confocal	907:914	arg1	CLSM					943:946	CLSM	943:946	CLSM	943:946	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	5	31	theme	confocal	907:914	arg1	microscopy					931:940	confocal laser scanning microscopy	907:940	confocal laser scanning microscopy (CLSM)	907:947	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	2	32	theme	components	409:418	arg1	synthesis					392:400	the synthesis	388:400	the synthesis of the components of the biofilm matrix	388:440	Biofilm is a complex system, influenced by nutritional-related factors that regulate the synthesis of the components of the biofilm matrix.
36532477	0	33	theme	compositional	12:24	arg1	analysis					26:33	Genetic and compositional analysis	0:33	Genetic and compositional analysis of biofilm	0:44	Genetic and compositional analysis of biofilm formed by Staphylococcus aureus isolated from food contact surfaces.
36532477	1	34	theme	food	269:272	arg1	safety					274:279	a serious food safety, and quality concern	259:300	safety	274:279	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	5	35	theme	scanning	922:929	arg1	CLSM					943:946	CLSM	943:946	CLSM	943:946	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	5	35	theme	scanning	922:929	arg1	microscopy					931:940	confocal laser scanning microscopy	907:940	confocal laser scanning microscopy (CLSM)	907:947	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	9	36	theme	high	1337:1340	arg1	density					1347:1353	a high cell density	1335:1353	a high cell density in all culture media	1335:1374	In particular, the biofilm formed by bap-positive S. aureus onto SS showed a high cell density in all culture media at 192 h in comparison with the biofilms formed at 24 h (p < 0.05).
36532477	2	37	theme	matrix	435:440	arg1	components					409:418	the components	405:418	the components of the biofilm matrix	405:440	Biofilm is a complex system, influenced by nutritional-related factors that regulate the synthesis of the components of the biofilm matrix.
36532477	10	38	theme	biofilm	1547:1553	arg1	formation					1555:1563	biofilm formation	1547:1563	biofilm formation	1547:1563	Epifluorescence microscopy and SEM revealed the metabolically active cells and the different stages of biofilm formation.
36532477	4	39	theme	electron	793:800	arg1	SEM					814:816	SEM	814:816	SEM	814:816	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	4	39	theme	electron	793:800	arg1	microscopy					802:811	scanning electron microscopy	784:811	scanning electron microscopy (SEM)	784:817	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	1	40	theme	quality	286:292	arg1	concern					294:300	a serious food safety, and quality concern	259:300	concern	294:300	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	0	41	attach	isolated	78:85	arg1	surfaces					105:112	food contact surfaces	92:112	food contact surfaces	92:112	Genetic and compositional analysis of biofilm formed by Staphylococcus aureus isolated from food contact surfaces.
36532477	0	41	attach	isolated	78:85	arg2	aureus					71:76	Staphylococcus aureus	56:76	Staphylococcus aureus isolated from food contact surfaces	56:112	Genetic and compositional analysis of biofilm formed by Staphylococcus aureus isolated from food contact surfaces.
36532477	6	42	theme	FCS-stainless	1060:1072	arg1	[n					1080:1081	FCS-stainless steel [n	1060:1081	FCS-stainless steel [n = 183	1060:1087	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	9	43	with	comparison	1388:1397	arg1	biofilms					1408:1415	the biofilms	1404:1415	the biofilms formed at 24 h (p < 0.05)	1404:1441	In particular, the biofilm formed by bap-positive S. aureus onto SS showed a high cell density in all culture media at 192 h in comparison with the biofilms formed at 24 h (p < 0.05).
36532477	11	44	theme	extracellular	1589:1601	arg1	polysaccharides					1670:1684	polysaccharides	1670:1684	polysaccharides	1670:1684	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	11	44	theme	extracellular	1589:1601	arg1	biofilms					1626:1633	extracellular polymeric of S. aureus biofilms	1589:1633	extracellular polymeric of S. aureus biofilms	1589:1633	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	11	44	theme	extracellular	1589:1601	arg1	proteins					1656:1663	proteins	1656:1663	proteins	1656:1663	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	11	44	theme	extracellular	1589:1601	arg1	eDNA					1650:1653	eDNA	1650:1653	eDNA	1650:1653	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	4	45	from	TSBGNaCl	683:690	arg1	SS					712:713	SS	712:713	SS	712:713	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	4	45	from	TSBGNaCl	683:690	arg1	stainless-steel					695:709	stainless-steel	695:709	stainless-steel (SS)	695:714	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	7	46	theme	genes	1165:1169	arg1	sigB					1171:1174	the genes sigB	1161:1174	the genes sigB (66%)	1161:1180	The isolates harbored the genes sigB (66%), sar (53%), agrD (52%), clfB/clfA (38%), fnbA/fnbB (20%), and bap (9.5%).
36532477	7	46	theme	genes	1165:1169	arg1	%					1179:1179	66%	1177:1179	66%	1177:1179	The isolates harbored the genes sigB (66%), sar (53%), agrD (52%), clfB/clfA (38%), fnbA/fnbB (20%), and bap (9.5%).
36532477	1	47	theme	dairy	236:240	arg1	industry					242:249	the dairy industry	232:249	the dairy industry	232:249	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	13	48	theme	p	2119:2119	arg1	<					2121:2121	p < 0.05	2119:2126	p < 0.05 at 24 h	2119:2134	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	14	49	from	industry	2323:2330	arg1	present					2302:2308	present	2302:2308	present	2302:2308	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	14	50	from	present	2302:2308	arg1	industry					2323:2330	the dairy industry	2313:2330	the dairy industry	2313:2330	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	14	51	theme	strategies	2416:2425	arg1	development					2352:2362	the development	2348:2362	the development of more efficient composition-specific disinfection strategies	2348:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	3	52	theme	different	544:552	arg1	conditions					561:570	different growth conditions	544:570	different growth conditions	544:570	This study determines the prevalence of biofilm-associated genes and evaluates the development under different growth conditions and compositions of biofilms produced by S. aureus.
36532477	1	53	theme	food	201:204	arg1	FCS					224:226	FCS	224:226	FCS	224:226	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	1	53	theme	food	201:204	arg1	surfaces					214:221	food contact surfaces	201:221	food contact surfaces (FCS)	201:227	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	14	54	theme	present	2302:2308	arg1	biofilms					2293:2300	S. aureus biofilms	2283:2300	S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies	2283:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	4	55	theme	Methods	624:630	arg1	Biofilms					632:639	Methods Biofilms	624:639	Methods Biofilms	624:639	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	14	56	theme	composition-specific	2382:2401	arg1	strategies					2416:2425	more efficient composition-specific disinfection strategies	2367:2425	more efficient composition-specific disinfection strategies	2367:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	3	57	theme	biofilms	592:599	arg1	compositions					576:587	compositions	576:587	compositions of biofilms produced by S. aureus	576:621	This study determines the prevalence of biofilm-associated genes and evaluates the development under different growth conditions and compositions of biofilms produced by S. aureus.
36532477	3	57	theme	biofilms	592:599	arg1	conditions					561:570	different growth conditions	544:570	different growth conditions	544:570	This study determines the prevalence of biofilm-associated genes and evaluates the development under different growth conditions and compositions of biofilms produced by S. aureus.
36532477	14	58	theme	aureus	2286:2291	arg1	biofilms					2293:2300	S. aureus biofilms	2283:2300	S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies	2283:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	10	59	theme	active	1506:1511	arg1	cells					1513:1517	the metabolically active cells	1488:1517	the metabolically active cells	1488:1517	Epifluorescence microscopy and SEM revealed the metabolically active cells and the different stages of biofilm formation.
36532477	5	60	theme	enzymatic	869:877	arg1	treatments					892:901	enzymatic and chemical treatments	869:901	enzymatic and chemical treatments	869:901	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	9	61	theme	p	1433:1433	arg1	h					1430:1430	24 h	1427:1430	24 h (p < 0.05)	1427:1441	In particular, the biofilm formed by bap-positive S. aureus onto SS showed a high cell density in all culture media at 192 h in comparison with the biofilms formed at 24 h (p < 0.05).
36532477	9	61	theme	p	1433:1433	arg1	<					1435:1435	p < 0.05	1433:1440	p < 0.05	1433:1440	In particular, the biofilm formed by bap-positive S. aureus onto SS showed a high cell density in all culture media at 192 h in comparison with the biofilms formed at 24 h (p < 0.05).
36532477	13	62	theme	detachment	2063:2072	arg1	level					2046:2050	a lower level	2038:2050	a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h)	2038:2135	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	5	63	theme	chemical	883:890	arg1	treatments					892:901	enzymatic and chemical treatments	869:901	enzymatic and chemical treatments	869:901	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	12	64	theme	culture	1934:1940	arg1	medium					1942:1947	the culture medium	1930:1947	the culture medium	1930:1947	Finally, the level of detachment on being treated with DNase I (44.7%) and NaIO 4(42.4%) was greater in the biofilms developed in TSB compared to culture medium supplemented with NaCl at 24 h; however, there was no significant difference when the culture medium was supplemented with glucose.
36532477	10	65	theme	different	1527:1535	arg1	stages					1537:1542	the different stages	1523:1542	the different stages of biofilm formation	1523:1563	Epifluorescence microscopy and SEM revealed the metabolically active cells and the different stages of biofilm formation.
36532477	5	66	theme	biofilms	839:846	arg1	composition					824:834	The composition	820:834	The composition of biofilms	820:846	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	6	67	dep	Results	950:956	arg1	total					975:979	A total	973:979	Results and discussion A total of 84 S. aureus (SA1-SA84) strains	950:1014	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	12	68	theme	significant	1902:1912	arg1	difference					1914:1923	no significant difference	1899:1923	no significant difference	1899:1923	Finally, the level of detachment on being treated with DNase I (44.7%) and NaIO 4(42.4%) was greater in the biofilms developed in TSB compared to culture medium supplemented with NaCl at 24 h; however, there was no significant difference when the culture medium was supplemented with glucose.
36532477	0	69	theme	contact	97:103	arg1	surfaces					105:112	food contact surfaces	92:112	food contact surfaces	92:112	Genetic and compositional analysis of biofilm formed by Staphylococcus aureus isolated from food contact surfaces.
36532477	4	70	theme	192	744:746	arg1	h					748:748	24 and 192 h	737:748	h	748:748	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	13	71	theme	lower	2040:2044	arg1	level					2046:2050	a lower level	2038:2050	a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h)	2038:2135	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	14	72	theme	deep	2246:2249	arg1	insight					2251:2257	a deep insight	2244:2257	a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies	2244:2425	These results represent a deep insight into the composition of S. aureus biofilms present in the dairy industry, which promotes the development of more efficient composition-specific disinfection strategies.
36532477	4	73	theme	24	737:738	arg1	h					748:748	24 and 192 h	737:748	h	748:748	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	13	74	theme	proteinase	2014:2023	arg1	K					2025:2025	proteinase K	2014:2025	proteinase K	2014:2025	In addition, after treatment with proteinase K, there was a lower level of biomass detachment (17.7%) of the biofilm developed in TSBNaCl (p < 0.05 at 24 h) compared to that in TSB, TSBG, and TSBGNaCl (33.6, 36.9, and 37.8%, respectively).
36532477	6	75	theme	strains	1008:1014	arg1	total					975:979	A total	973:979	Results and discussion A total of 84 S. aureus (SA1-SA84) strains	950:1014	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	1	76	theme	serious	261:267	arg1	safety					274:279	a serious food safety, and quality concern	259:300	safety	274:279	Introduction Staphylococcus aureus is an important pathogen that can form biofilms on food contact surfaces (FCS) in the dairy industry, posing a serious food safety, and quality concern.
36532477	5	77	theme	laser	916:920	arg1	CLSM					943:946	CLSM	943:946	CLSM	943:946	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	5	77	theme	laser	916:920	arg1	microscopy					931:940	confocal laser scanning microscopy	907:940	confocal laser scanning microscopy (CLSM)	907:947	The composition of biofilms was determined using enzymatic and chemical treatments and confocal laser scanning microscopy (CLSM).
36532477	9	78	from	density	1347:1353	arg1	media					1370:1374	all culture media	1358:1374	all culture media	1358:1374	In particular, the biofilm formed by bap-positive S. aureus onto SS showed a high cell density in all culture media at 192 h in comparison with the biofilms formed at 24 h (p < 0.05).
36532477	3	79	theme	genes	502:506	arg1	prevalence					469:478	the prevalence	465:478	the prevalence of biofilm-associated genes	465:506	This study determines the prevalence of biofilm-associated genes and evaluates the development under different growth conditions and compositions of biofilms produced by S. aureus.
36532477	0	80	theme	biofilm	38:44	arg1	analysis					26:33	Genetic and compositional analysis	0:33	Genetic and compositional analysis of biofilm	0:44	Genetic and compositional analysis of biofilm formed by Staphylococcus aureus isolated from food contact surfaces.
36532477	6	81	theme	aureus	990:995	arg1	strains					1008:1014	84 S. aureus (SA1-SA84) strains	984:1014	84 S. aureus (SA1-SA84) strains	984:1014	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	10	82	theme	formation	1555:1563	arg1	cells					1513:1517	the metabolically active cells	1488:1517	the metabolically active cells	1488:1517	Epifluorescence microscopy and SEM revealed the metabolically active cells and the different stages of biofilm formation.
36532477	10	82	theme	formation	1555:1563	arg1	stages					1537:1542	the different stages	1523:1542	the different stages of biofilm formation	1523:1563	Epifluorescence microscopy and SEM revealed the metabolically active cells and the different stages of biofilm formation.
36532477	9	83	theme	cell	1342:1345	arg1	density					1347:1353	a high cell density	1335:1353	a high cell density in all culture media	1335:1374	In particular, the biofilm formed by bap-positive S. aureus onto SS showed a high cell density in all culture media at 192 h in comparison with the biofilms formed at 24 h (p < 0.05).
36532477	6	84	theme	steel	1074:1078	arg1	[n					1080:1081	FCS-stainless steel [n	1060:1081	FCS-stainless steel [n = 183	1060:1087	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	2	85	theme	biofilm	427:433	arg1	matrix					435:440	the biofilm matrix	423:440	the biofilm matrix	423:440	Biofilm is a complex system, influenced by nutritional-related factors that regulate the synthesis of the components of the biofilm matrix.
36532477	4	86	theme	scanning	784:791	arg1	SEM					814:816	SEM	814:816	SEM	814:816	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	4	86	theme	scanning	784:791	arg1	microscopy					802:811	scanning electron microscopy	784:811	scanning electron microscopy (SEM)	784:817	Methods Biofilms were developed in TSB, TSBG, TSBNaCl, and TSBGNaCl on stainless-steel (SS), with enumeration at 24 and 192 h visualized by epifluorescence and scanning electron microscopy (SEM).
36532477	3	87	theme	biofilm-associated	483:500	arg1	genes					502:506	biofilm-associated genes	483:506	biofilm-associated genes	483:506	This study determines the prevalence of biofilm-associated genes and evaluates the development under different growth conditions and compositions of biofilms produced by S. aureus.
36532477	11	88	theme	CLSM	1566:1569	arg1	analysis					1571:1578	CLSM analysis	1566:1578	CLSM analysis	1566:1578	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	6	89	dep	FCS	1055:1057	arg1	[n					1080:1081	FCS-stainless steel [n	1060:1081	FCS-stainless steel [n = 183	1060:1087	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	6	90	theme	dairy	1040:1044	arg1	FCS					1055:1057	293 dairy industry FCS	1036:1057	293 dairy industry FCS (FCS-stainless steel [n = 183]	1036:1088	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	12	91	theme	detachment	1709:1718	arg1	greater					1780:1786	greater	1780:1786	greater	1780:1786	Finally, the level of detachment on being treated with DNase I (44.7%) and NaIO 4(42.4%) was greater in the biofilms developed in TSB compared to culture medium supplemented with NaCl at 24 h; however, there was no significant difference when the culture medium was supplemented with glucose.
36532477	12	91	theme	detachment	1709:1718	arg1	level					1700:1704	the level	1696:1704	the level of detachment on being treated with DNase I (44.7%) and NaIO 4(42.4%)	1696:1774	Finally, the level of detachment on being treated with DNase I (44.7%) and NaIO 4(42.4%) was greater in the biofilms developed in TSB compared to culture medium supplemented with NaCl at 24 h; however, there was no significant difference when the culture medium was supplemented with glucose.
36532477	9	92	theme	culture	1362:1368	arg1	media					1370:1374	all culture media	1358:1374	all culture media	1358:1374	In particular, the biofilm formed by bap-positive S. aureus onto SS showed a high cell density in all culture media at 192 h in comparison with the biofilms formed at 24 h (p < 0.05).
36532477	6	93	theme	industry	1046:1053	arg1	FCS					1055:1057	293 dairy industry FCS	1036:1057	293 dairy industry FCS (FCS-stainless steel [n = 183]	1036:1088	Results and discussion A total of 84 S. aureus (SA1-SA84) strains were collected from 293 dairy industry FCS (FCS-stainless steel [n = 183] and FCS-polypropylene [n = 110]) for this study.
36532477	11	94	theme	polymeric	1603:1611	arg1	polysaccharides					1670:1684	polysaccharides	1670:1684	polysaccharides	1670:1684	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	11	94	theme	polymeric	1603:1611	arg1	biofilms					1626:1633	extracellular polymeric of S. aureus biofilms	1589:1633	extracellular polymeric of S. aureus biofilms	1589:1633	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	11	94	theme	polymeric	1603:1611	arg1	proteins					1656:1663	proteins	1656:1663	proteins	1656:1663	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36532477	11	94	theme	polymeric	1603:1611	arg1	eDNA					1650:1653	eDNA	1650:1653	eDNA	1650:1653	CLSM analysis detected extracellular polymeric of S. aureus biofilms on SS, such as eDNA, proteins, and polysaccharides.
36963682	6	0	theme	great	1539:1543	arg1	composition					1551:1561	great lipid composition	1539:1561	great lipid composition	1539:1561	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	3	1	theme	discharge	727:735	arg1	standards					737:745	the discharge standards	723:745	the discharge standards	723:745	The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards.
36963682	5	2	theme	nutrients	1390:1398	arg1	removal					1400:1406	nutrients removal	1390:1406	nutrients removal	1390:1406	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	2	3	theme	pyrenoidosa	396:406	arg1	metabolism					372:381	nitrogen metabolism	363:381	nitrogen metabolism	363:381	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	2	3	theme	pyrenoidosa	396:406	arg1	carbon					352:357	carbon	352:357	carbon	352:357	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	7	4	theme	microalgae	1739:1748	arg1	metabolism					1725:1734	the carbon and nitrogen metabolism	1701:1734	metabolism	1725:1734	This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.
36963682	5	5	theme	tricarboxylic	1201:1213	arg1	cycle					1215:1219	tricarboxylic cycle	1201:1219	tricarboxylic cycle	1201:1219	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	0	6	theme	aquaculture	77:87	arg1	wastewater					89:98	aquaculture wastewater	77:98	aquaculture wastewater	77:98	Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors.
36963682	5	7	theme	red	1128:1130	arg1	light					1132:1136	red light	1128:1136	red light	1128:1136	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	8	theme	molecular	1053:1061	arg1	level					1063:1067	a molecular level	1051:1067	a molecular level	1051:1067	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	9	theme	cycle	1215:1219	arg1	flux					1154:1157	carbon flux	1147:1157	carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle	1147:1219	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	4	10	dep	standards	993:1001	arg1	the					979:981	the	979:981	the	979:981	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	3	11	theme	red	576:578	arg1	light					584:588	The red LED light	572:588	The red LED light	572:588	The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards.
36963682	1	12	theme	strict	285:290	arg1	standards					302:310	the strict discharge standards	281:310	the strict discharge standards	281:310	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	2	13	theme	plate	549:553	arg1	photobioreactor					555:569	a plate photobioreactor	547:569	a plate photobioreactor	547:569	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	3	14	theme	LED	580:582	arg1	light					584:588	The red LED light	572:588	The red LED light	572:588	The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards.
36963682	1	15	theme	discharge	292:300	arg1	standards					302:310	the strict discharge standards	281:310	the strict discharge standards	281:310	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	4	16	dep	%	920:920	arg1	met					975:977	met	975:977	met	975:977	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	17	theme	total	861:865	arg1	nitrogen					875:882	total ammonia nitrogen	861:882	total ammonia nitrogen	861:882	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	2	18	theme	starch	446:451	arg1	addition					453:460	starch addition	446:460	starch addition	446:460	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	4	19	theme	phosphorus	805:814	arg1	%					920:920	85.15, 96.96, 88.53, and 98.01 %	889:920	85.15, 96.96, 88.53, and 98.01 %	889:920	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	19	theme	phosphorus	805:814	arg1	efficiencies					783:794	the removal efficiencies	771:794	the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen	771:882	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	2	20	theme	LED	432:434	arg1	light					436:440	the red LED light	424:440	the red LED light	424:440	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	0	21	theme	high-quality	112:123	arg1	protein					125:131	high-quality protein	112:131	high-quality protein in plate photobioreactors	112:157	Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors.
36963682	5	22	theme	transcriptome	1093:1105	arg1	analyses					1107:1114	the metabolic flux and transcriptome analyses	1070:1114	analyses	1107:1114	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	4	23	theme	protein	1011:1017	arg1	production					1019:1028	the protein production	1007:1028	the protein production	1007:1028	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	5	24	theme	ammonium	1350:1357	arg1	genes					1252:1256	genes	1252:1256	genes encoding α-amylase	1252:1275	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	24	theme	ammonium	1350:1357	arg1	transporter					1359:1369	ammonium transporter	1350:1369	ammonium transporter	1350:1369	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	2	25	theme	aquaculture	479:489	arg1	T-AW					503:506	T-AW	503:506	T-AW	503:506	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	2	25	theme	aquaculture	479:489	arg1	wastewater					491:500	Tilapia aquaculture wastewater	471:500	Tilapia aquaculture wastewater (T-AW)	471:507	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	4	26	theme	nitrogen	875:882	arg1	%					920:920	85.15, 96.96, 88.53, and 98.01 %	889:920	85.15, 96.96, 88.53, and 98.01 %	889:920	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	26	theme	nitrogen	875:882	arg1	efficiencies					783:794	the removal efficiencies	771:794	the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen	771:882	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	3	27	from	temperature	649:659	arg1	removals					626:633	the nutrient removals	613:633	the nutrient removals at an outdoor temperature	613:659	The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards.
36963682	1	28	theme	economical	202:211	arg1	treatment					171:179	Wastewater treatment	160:179	Wastewater treatment by microalgae	160:193	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	1	28	theme	economical	202:211	arg1	strategy					242:249	the economical and environmentally friendly strategy	198:249	the economical and environmentally friendly strategy	198:249	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	2	29	theme	Tilapia	471:477	arg1	T-AW					503:506	T-AW	503:506	T-AW	503:506	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	2	29	theme	Tilapia	471:477	arg1	wastewater					491:500	Tilapia aquaculture wastewater	471:500	Tilapia aquaculture wastewater (T-AW)	471:507	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	4	30	theme	ammonia	867:873	arg1	nitrogen					875:882	total ammonia nitrogen	861:882	total ammonia nitrogen	861:882	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	5	31	theme	protein	1442:1448	arg1	biosynthesis					1450:1461	protein biosynthesis	1442:1461	protein biosynthesis	1442:1461	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	7	32	theme	nitrogen	1716:1723	arg1	metabolism					1725:1734	the carbon and nitrogen metabolism	1701:1734	metabolism	1725:1734	This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.
36963682	0	33	theme	nitrogen	23:30	arg1	metabolism					32:41	nitrogen metabolism	23:41	nitrogen metabolism	23:41	Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors.
36963682	5	34	theme	Embden-Meyerhof-Pranas	1166:1187	arg1	pathway					1189:1195	the Embden-Meyerhof-Pranas pathway	1162:1195	the Embden-Meyerhof-Pranas pathway	1162:1195	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	0	35	theme	plate	136:140	arg1	photobioreactors					142:157	plate photobioreactors	136:157	plate photobioreactors	136:157	Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors.
36963682	4	36	theme	removal	775:781	arg1	%					920:920	85.15, 96.96, 88.53, and 98.01 %	889:920	85.15, 96.96, 88.53, and 98.01 %	889:920	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	36	theme	removal	775:781	arg1	efficiencies					783:794	the removal efficiencies	771:794	the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen	771:882	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	37	theme	oxygen	842:847	arg1	demand					849:854	chemical oxygen demand	833:854	chemical oxygen demand	833:854	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	1	38	theme	environmentally	217:231	arg1	treatment					171:179	Wastewater treatment	160:179	Wastewater treatment by microalgae	160:193	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	1	38	theme	environmentally	217:231	arg1	strategy					242:249	the economical and environmentally friendly strategy	198:249	the economical and environmentally friendly strategy	198:249	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	7	39	theme	carbon	1705:1710	arg1	metabolism					1725:1734	the carbon and nitrogen metabolism	1701:1734	metabolism	1725:1734	This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.
36963682	1	40	theme	valuable	316:323	arg1	exploitations					333:345	valuable biomass exploitations	316:345	valuable biomass exploitations	316:345	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	5	41	theme	pathway	1189:1195	arg1	flux					1154:1157	carbon flux	1147:1157	carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle	1147:1219	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	42	theme	nitrate	1325:1331	arg1	genes					1252:1256	genes	1252:1256	genes encoding α-amylase	1252:1275	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	42	theme	nitrate	1325:1331	arg1	transporter					1333:1343	nitrate transporter	1325:1343	nitrate transporter	1325:1343	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	1	43	theme	friendly	233:240	arg1	treatment					171:179	Wastewater treatment	160:179	Wastewater treatment by microalgae	160:193	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	1	43	theme	friendly	233:240	arg1	strategy					242:249	the economical and environmentally friendly strategy	198:249	the economical and environmentally friendly strategy	198:249	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	1	44	theme	biomass	325:331	arg1	exploitations					333:345	valuable biomass exploitations	316:345	valuable biomass exploitations	316:345	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	3	45	theme	nutrient	617:624	arg1	removals					626:633	the nutrient removals	613:633	the nutrient removals at an outdoor temperature	613:659	The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards.
36963682	6	46	theme	%	1511:1511	arg1	acids					1529:1533	the 62 % essential amino acids	1504:1533	the 62 % essential amino acids	1504:1533	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	4	47	theme	discharge	983:991	arg1	standards					993:1001	discharge standards	983:1001	discharge standards	983:1001	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	7	48	theme	protein	1652:1658	arg1	production					1660:1669	protein production	1652:1669	protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae	1652:1748	This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.
36963682	7	49	theme	new	1599:1601	arg1	orientation					1603:1613	a new orientation	1597:1613	a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae	1597:1748	This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.
36963682	4	50	from	%	920:920	arg1	photobioreactor					938:952	a flat-plate photobioreactor	925:952	a flat-plate photobioreactor	925:952	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	51	theme	chemical	833:840	arg1	demand					849:854	chemical oxygen demand	833:854	chemical oxygen demand	833:854	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	0	52	theme	pyrenoidosa	56:66	arg1	carbon					12:17	carbon	12:17	carbon	12:17	Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors.
36963682	0	52	theme	pyrenoidosa	56:66	arg1	metabolism					32:41	nitrogen metabolism	23:41	nitrogen metabolism	23:41	Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors.
36963682	5	53	theme	carbon	1147:1152	arg1	flux					1154:1157	carbon flux	1147:1157	carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle	1147:1219	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	54	theme	metabolic	1074:1082	arg1	flux					1084:1087	the metabolic flux and transcriptome analyses	1070:1114	flux	1084:1087	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	6	55	theme	harvesting	1468:1477	arg1	pyrenoidosa					1482:1492	The harvesting C. pyrenoidosa	1464:1492	The harvesting C. pyrenoidosa	1464:1492	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	6	56	contain	possessed	1494:1502	arg2	acids					1529:1533	the 62 % essential amino acids	1504:1533	the 62 % essential amino acids	1504:1533	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	6	56	contain	possessed	1494:1502	arg1	pyrenoidosa					1482:1492	The harvesting C. pyrenoidosa	1464:1492	The harvesting C. pyrenoidosa	1464:1492	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	6	56	contain	possessed	1494:1502	arg2	composition					1551:1561	great lipid composition	1539:1561	great lipid composition	1539:1561	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	0	57	from	protein	125:131	arg1	photobioreactors					142:157	plate photobioreactors	136:157	plate photobioreactors	136:157	Stimulating carbon and nitrogen metabolism of Chlorella pyrenoidosa to treat aquaculture wastewater and produce high-quality protein in plate photobioreactors.
36963682	4	58	theme	demand	849:854	arg1	%					920:920	85.15, 96.96, 88.53, and 98.01 %	889:920	85.15, 96.96, 88.53, and 98.01 %	889:920	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	58	theme	demand	849:854	arg1	efficiencies					783:794	the removal efficiencies	771:794	the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen	771:882	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	59	theme	starch	754:759	arg1	addition					761:768	starch addition	754:768	starch addition	754:768	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	5	60	theme	glutamine	1278:1286	arg1	synthetase					1288:1297	glutamine synthetase	1278:1297	glutamine synthetase	1278:1297	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	60	theme	glutamine	1278:1286	arg1	genes					1252:1256	genes	1252:1256	genes encoding α-amylase	1252:1275	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	4	61	theme	total	817:821	arg1	nitrogen					823:830	total nitrogen	817:830	total nitrogen	817:830	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	3	62	theme	outdoor	641:647	arg1	temperature					649:659	an outdoor temperature	638:659	an outdoor temperature	638:659	The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards.
36963682	5	63	theme	nitrogen	1421:1428	arg1	sources					1430:1436	nitrogen sources	1421:1436	nitrogen sources for protein biosynthesis	1421:1461	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	2	64	theme	nitrogen	363:370	arg1	metabolism					372:381	nitrogen metabolism	363:381	nitrogen metabolism	363:381	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	6	65	theme	amino	1523:1527	arg1	acids					1529:1533	the 62 % essential amino acids	1504:1533	the 62 % essential amino acids	1504:1533	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	7	66	theme	outdoor	1619:1625	arg1	treatment					1638:1646	outdoor wastewater treatment	1619:1646	outdoor wastewater treatment	1619:1646	This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.
36963682	4	67	theme	total	799:803	arg1	phosphorus					805:814	total phosphorus	799:814	total phosphorus	799:814	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	6	68	theme	lipid	1545:1549	arg1	composition					1551:1561	great lipid composition	1539:1561	great lipid composition	1539:1561	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	6	69	theme	essential	1513:1521	arg1	acids					1529:1533	the 62 % essential amino acids	1504:1533	the 62 % essential amino acids	1504:1533	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	2	70	dep	carbon	352:357	arg1	The					348:350	The	348:350	The	348:350	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	7	71	theme	wastewater	1627:1636	arg1	treatment					1638:1646	outdoor wastewater treatment	1619:1646	outdoor wastewater treatment	1619:1646	This study provided a new orientation for outdoor wastewater treatment and protein production by collaboratively regulating the carbon and nitrogen metabolism of microalgae.
36963682	3	72	theme	total	692:696	arg1	nitrogen					698:705	total nitrogen	692:705	total nitrogen	692:705	The red LED light was applied to improve the nutrient removals at an outdoor temperature, but the concentrations except total nitrogen did not satisfy the discharge standards.
36963682	2	73	dep	LED	432:434	arg1	red					428:430	red	428:430	red	428:430	The carbon and nitrogen metabolism of Chlorella pyrenoidosa was improved by the red LED light and starch addition to treat Tilapia aquaculture wastewater (T-AW) and produce protein simultaneously in a plate photobioreactor.
36963682	6	74	theme	62 	1508:1510	arg1	%					1511:1511	%	1511:1511	%	1511:1511	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	1	75	theme	Wastewater	160:169	arg1	treatment					171:179	Wastewater treatment	160:179	Wastewater treatment by microalgae	160:193	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	1	75	theme	Wastewater	160:169	arg1	strategy					242:249	the economical and environmentally friendly strategy	198:249	the economical and environmentally friendly strategy	198:249	Wastewater treatment by microalgae is the economical and environmentally friendly strategy, but is still challenged with the strict discharge standards and valuable biomass exploitations.
36963682	5	76	theme	glutamate	1300:1308	arg1	dehydrogenase					1310:1322	glutamate dehydrogenase	1300:1322	glutamate dehydrogenase	1300:1322	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	5	76	theme	glutamate	1300:1308	arg1	genes					1252:1256	genes	1252:1256	genes encoding α-amylase	1252:1275	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	4	77	theme	nitrogen	823:830	arg1	%					920:920	85.15, 96.96, 88.53, and 98.01 %	889:920	85.15, 96.96, 88.53, and 98.01 %	889:920	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	4	77	theme	nitrogen	823:830	arg1	efficiencies					783:794	the removal efficiencies	771:794	the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen	771:882	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
36963682	6	78	theme	C.	1479:1480	arg1	pyrenoidosa					1482:1492	The harvesting C. pyrenoidosa	1464:1492	The harvesting C. pyrenoidosa	1464:1492	The harvesting C. pyrenoidosa possessed the 62 % essential amino acids and great lipid composition for biofuels.
36963682	5	79	theme	genes	1252:1256	arg1	levels					1242:1247	the levels	1238:1247	the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis	1238:1461	At a molecular level, the metabolic flux and transcriptome analyses showed that red light promoted carbon flux of the Embden-Meyerhof-Pranas pathway and tricarboxylic cycle, and upregulated the levels of genes encoding α-amylase, glutamine synthetase, glutamate dehydrogenase, nitrate transporter, and ammonium transporter, which facilitated nutrients removal and provided nitrogen sources for protein biosynthesis.
36963682	4	80	theme	flat-plate	927:936	arg1	photobioreactor					938:952	a flat-plate photobioreactor	925:952	a flat-plate photobioreactor	925:952	After starch addition, the removal efficiencies of total phosphorus, total nitrogen, chemical oxygen demand, and total ammonia nitrogen were 85.15, 96.96, 88.53, and 98.01 % in a flat-plate photobioreactor, respectively, which met the discharge standards and the protein production reached 0.60 g/L.
35092364	1	0	attach	linked	120:125	arg1	facets					139:144	multiple facets	130:144	multiple facets of human physiology	130:164	The human microbiome has been inextricably linked to multiple facets of human physiology.
35092364	1	0	attach	linked	120:125	arg2	microbiome					87:96	The human microbiome	77:96	The human microbiome	77:96	The human microbiome has been inextricably linked to multiple facets of human physiology.
35092364	2	1	theme	new	360:362	arg1	avenues					364:370	new avenues	360:370	new avenues of therapeutic and diagnostic agents	360:407	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	3	2	from	studies	529:535	arg1	microbiome					544:553	the microbiome	540:553	the microbiome	540:553	While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions.
35092364	4	3	attach	presented	722:730	arg1	Conference					655:664	the 3rd International Conference	633:664	the 3rd International Conference on Microbiome Engineering	633:690	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	4	3	attach	presented	722:730	arg2	experts					701:707	leading experts	693:707	leading experts in the field	693:720	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	4	4	from	experts	701:707	arg1	field					716:720	the field	712:720	the field	712:720	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	4	5	theme	Microbiome	669:678	arg1	Engineering					680:690	Microbiome Engineering	669:690	Microbiome Engineering	669:690	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	2	6	theme	great	285:289	arg1	promise					291:297	great promise	285:297	great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents	285:407	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	4	7	theme	state-of-the-art	732:747	arg1	work					749:752	state-of-the-art work	732:752	state-of-the-art work in microbiome engineering	732:778	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	3	8	theme	new	525:527	arg1	studies					529:535	new studies	525:535	new studies in the microbiome	525:553	While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions.
35092364	3	9	theme	research	440:447	arg1	field					420:424	the field	416:424	the field of microbiome research	416:447	While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions.
35092364	0	10	from	Conference	39:48	arg1	Proceedings					0:10	Proceedings	0:10	Proceedings from the 3rd International Conference on Microbiome Engineering	0:74	Proceedings from the 3rd International Conference on Microbiome Engineering.
35092364	0	10	from	Conference	39:48	arg1	Engineering					64:74	Microbiome Engineering	53:74	Microbiome Engineering	53:74	Proceedings from the 3rd International Conference on Microbiome Engineering.
35092364	1	11	theme	human	81:85	arg1	microbiome					87:96	The human microbiome	77:96	The human microbiome	77:96	The human microbiome has been inextricably linked to multiple facets of human physiology.
35092364	2	12	theme	diagnostic	391:400	arg1	agents					402:407	therapeutic and diagnostic agents	375:407	therapeutic and diagnostic agents	375:407	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	4	13	theme	microbiome	757:766	arg1	engineering					768:778	microbiome engineering	757:778	microbiome engineering	757:778	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	4	14	link	derived	849:855	arg1	biomolecules					857:868	microbially derived biomolecules	837:868	microbially derived biomolecules	837:868	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	4	15	from	Conference	655:664	arg1	Engineering					680:690	Microbiome Engineering	669:690	Microbiome Engineering	669:690	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	3	16	theme	microbiome	429:438	arg1	research					440:447	microbiome research	429:447	microbiome research	429:447	While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions.
35092364	0	17	theme	3rd	21:23	arg1	Conference					39:48	the 3rd International Conference	17:48	the 3rd International Conference on Microbiome Engineering	17:74	Proceedings from the 3rd International Conference on Microbiome Engineering.
35092364	4	18	theme	3rd	637:639	arg1	Conference					655:664	the 3rd International Conference	633:664	the 3rd International Conference on Microbiome Engineering	633:690	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	2	19	theme	engineering	175:185	arg1	standpoint					187:196	an engineering standpoint	172:196	an engineering standpoint	172:196	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	4	20	theme	derived	849:855	arg1	biomolecules					857:868	microbially derived biomolecules	837:868	microbially derived biomolecules	837:868	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	2	21	theme	therapeutic	375:385	arg1	agents					402:407	therapeutic and diagnostic agents	375:407	therapeutic and diagnostic agents	375:407	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	2	22	dep	composition	236:246	arg1	the					232:234	the	232:234	the	232:234	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	2	23	theme	agents	402:407	arg1	avenues					364:370	new avenues	360:370	new avenues of therapeutic and diagnostic agents	360:407	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	0	24	theme	International	25:37	arg1	Conference					39:48	the 3rd International Conference	17:48	the 3rd International Conference on Microbiome Engineering	17:74	Proceedings from the 3rd International Conference on Microbiome Engineering.
35092364	4	25	theme	International	641:653	arg1	Conference					655:664	the 3rd International Conference	633:664	the 3rd International Conference on Microbiome Engineering	633:690	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	0	26	theme	Microbiome	53:62	arg1	Engineering					64:74	Microbiome Engineering	53:74	Microbiome Engineering	53:74	Proceedings from the 3rd International Conference on Microbiome Engineering.
35092364	3	27	theme	engineering	482:492	arg1	efforts					494:500	growing engineering efforts	474:500	growing engineering efforts	474:500	While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions.
35092364	2	28	theme	etiology	343:350	arg1	understanding					318:330	our understanding	314:330	our understanding of disease etiology	314:350	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	4	29	from	work	749:752	arg1	engineering					768:778	microbiome engineering	757:778	microbiome engineering	757:778	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	4	30	theme	engineered	816:825	arg1	microbes					827:834	engineered microbes	816:834	engineered microbes	816:834	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	4	31	theme	leading	693:699	arg1	experts					701:707	leading experts	693:707	leading experts in the field	693:720	At the 3rd International Conference on Microbiome Engineering, leading experts in the field presented state-of-the-art work in microbiome engineering, discussing probiotics, prebiotics, engineered microbes, microbially derived biomolecules, and bacteriophage.
35092364	1	32	theme	multiple	130:137	arg1	facets					139:144	multiple facets	130:144	multiple facets of human physiology	130:164	The human microbiome has been inextricably linked to multiple facets of human physiology.
35092364	2	33	theme	microbiome	268:277	arg1	activity					252:259	activity	252:259	activity	252:259	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	2	33	theme	microbiome	268:277	arg1	composition					236:246	composition	236:246	composition	236:246	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	3	34	theme	growing	474:480	arg1	efforts					494:500	growing engineering efforts	474:500	growing engineering efforts	474:500	While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions.
35092364	2	35	theme	disease	335:341	arg1	etiology					343:350	disease etiology	335:350	disease etiology	335:350	From an engineering standpoint, the ability to precisely control the composition and activity of the microbiome holds great promise for furthering our understanding of disease etiology and for new avenues of therapeutic and diagnostic agents.
35092364	3	36	theme	microbiome-based	598:613	arg1	interventions					615:627	microbiome-based interventions	598:627	microbiome-based interventions	598:627	While the field of microbiome research is still in its infancy, growing engineering efforts are emerging to enable new studies in the microbiome and to rapidly translate these findings to microbiome-based interventions.
35092364	1	37	theme	physiology	155:164	arg1	facets					139:144	multiple facets	130:144	multiple facets of human physiology	130:164	The human microbiome has been inextricably linked to multiple facets of human physiology.
35092364	1	38	theme	human	149:153	arg1	physiology					155:164	human physiology	149:164	human physiology	149:164	The human microbiome has been inextricably linked to multiple facets of human physiology.
34993771	0	0	theme	amoxicillin	98:108	arg1	removal					87:93	adsorptive removal	76:93	adsorptive removal of amoxicillin from aqueous medium	76:128	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	2	1	theme	precipitation	482:494	arg1	method					496:501	a facile precipitation method	473:501	a facile precipitation method	473:501	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	9	2	theme	BiOI-Ch	1768:1774	arg1	performance					1747:1757	improved performance	1738:1757	improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1738:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	3	3	theme	adsorbent	809:817	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	3	theme	adsorbent	809:817	arg1	dose					819:822	1.7 g L-1 adsorbent dose	799:822	1.7 g L-1 adsorbent dose	799:822	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	2	4	theme	facile	475:480	arg1	method					496:501	a facile precipitation method	473:501	a facile precipitation method	473:501	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	1	5	theme	water	354:358	arg1	bodies					360:365	water bodies	354:365	water bodies	354:365	Amoxicillin (AMX) is a widely used antibiotic, which induces harmful effects to nature via bioaccumulation and persistence in the environment if discharged untreated into water bodies.
34993771	8	6	theme	surface	1500:1506	arg1	area					1508:1511	surface area	1500:1511	enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1462:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	8	7	theme	functional	1471:1480	arg1	nanocomposite					1520:1532	the nanocomposite	1516:1532	the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1516:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	8	7	theme	functional	1471:1480	arg1	groups					1482:1487	enhanced functional groups	1462:1487	enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1462:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	3	8	theme	ultrasonication	847:861	arg1	20 min					868:873	ultrasonication time 20 min	847:873	ultrasonication time 20 min	847:873	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	8	theme	ultrasonication	847:861	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	0	9	theme	aqueous	115:121	arg1	medium					123:128	aqueous medium	115:128	aqueous medium	115:128	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	3	10	theme	maximum	696:702	arg1	adsorption					704:713	maximum adsorption	696:713	maximum adsorption of AMX	696:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	11	theme	time	863:866	arg1	20 min					868:873	ultrasonication time 20 min	847:873	ultrasonication time 20 min	847:873	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	11	theme	time	863:866	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	12	theme	1.7 g	799:803	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	12	theme	1.7 g	799:803	arg1	dose					819:822	1.7 g L-1 adsorbent dose	799:822	1.7 g L-1 adsorbent dose	799:822	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	5	13	theme	pure	1097:1100	arg1	33.78 mg g-1					1108:1119	33.78 mg g-1	1108:1119	33.78 mg g-1	1108:1119	BiOI-Ch exhibited superior adsorption capacity as compared to pure BiOI (33.78 mg g-1).
34993771	5	13	theme	pure	1097:1100	arg1	BiOI					1102:1105	pure BiOI	1097:1105	pure BiOI (33.78 mg g-1)	1097:1120	BiOI-Ch exhibited superior adsorption capacity as compared to pure BiOI (33.78 mg g-1).
34993771	2	14	theme	waves	579:583	arg1	presence					556:563	the presence	552:563	the presence of ultrasonic waves	552:583	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	8	15	theme	molecules	1652:1660	arg1	removal					1637:1643	the removal	1633:1643	the removal of AMX molecules	1633:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	3	16	theme	L-1	805:807	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	16	theme	L-1	805:807	arg1	dose					819:822	1.7 g L-1 adsorbent dose	799:822	1.7 g L-1 adsorbent dose	799:822	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	4	17	dep	AMX	912:914	arg1	%					910:910	%	910:910	%	910:910	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	3	18	theme	obtained	730:737	arg1	results					739:745	the obtained results	726:745	the obtained results were as follows	726:761	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	1	19	from	persistence	294:304	arg1	environment					313:323	the environment	309:323	the environment if discharged untreated into water bodies	309:365	Amoxicillin (AMX) is a widely used antibiotic, which induces harmful effects to nature via bioaccumulation and persistence in the environment if discharged untreated into water bodies.
34993771	9	20	from	effluent	1878:1885	arg1	pollutants					1851:1860	persistent and detrimental pollutants	1824:1860	persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1824:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	9	20	from	effluent	1878:1885	arg1	elimination					1809:1819	the elimination	1805:1819	the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1805:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	9	21	theme	large-scale	1920:1930	arg1	operation					1932:1940	large-scale operation	1920:1940	large-scale operation	1920:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	2	22	theme	novel	392:396	arg1	bionanocomposite					398:413	a novel bionanocomposite	390:413	a novel bionanocomposite	390:413	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	2	22	theme	novel	392:396	arg1	oxyiodide-chitosan					424:441	bismuth oxyiodide-chitosan	416:441	bismuth oxyiodide-chitosan (BiOI-Ch)	416:451	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	3	23	dep	parameters	681:690	arg1	concentration					784:796	80 mg g-1 AMX concentration	770:796	80 mg g-1 AMX concentration	770:796	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	23	dep	parameters	681:690	arg1	temperature					825:835	temperature 298 K	825:841	temperature 298 K	825:841	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	23	dep	parameters	681:690	arg1	pH					764:765	pH 3	764:767	pH 3	764:767	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	23	dep	parameters	681:690	arg1	dose					819:822	1.7 g L-1 adsorbent dose	799:822	1.7 g L-1 adsorbent dose	799:822	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	23	dep	parameters	681:690	arg1	20 min					868:873	ultrasonication time 20 min	847:873	ultrasonication time 20 min	847:873	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	23	dep	parameters	681:690	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	24	theme	AMX	718:720	arg1	adsorption					704:713	maximum adsorption	696:713	maximum adsorption of AMX	696:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	8	25	theme	AMX	1648:1650	arg1	molecules					1652:1660	AMX molecules	1648:1660	AMX molecules	1648:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	9	26	theme	detrimental	1839:1849	arg1	pollutants					1851:1860	persistent and detrimental pollutants	1824:1860	persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1824:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	8	27	theme	superior	1547:1554	arg1	capacity					1567:1574	superior adsorption capacity	1547:1574	superior adsorption capacity	1547:1574	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	1	28	from	bioaccumulation	274:288	arg1	environment					313:323	the environment	309:323	the environment if discharged untreated into water bodies	309:365	Amoxicillin (AMX) is a widely used antibiotic, which induces harmful effects to nature via bioaccumulation and persistence in the environment if discharged untreated into water bodies.
34993771	6	29	theme	reaction	1153:1160	arg1	dynamics					1141:1148	the dynamics	1137:1148	the dynamics of reaction	1137:1160	To understand the dynamics of reaction, several kinetic and isotherm models were also examined.
34993771	9	30	theme	synergistic	1697:1707	arg1	effect					1709:1714	the synergistic effect	1693:1714	the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1693:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	0	31	theme	New	0:2	arg1	bismuth					4:10	New bismuth	0:10	New bismuth	0:10	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	8	32	theme	fast	1577:1580	arg1	kinetics					1593:1600	fast adsorption kinetics	1577:1600	fast adsorption kinetics	1577:1600	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	5	33	theme	adsorption	1062:1071	arg1	capacity					1073:1080	superior adsorption capacity	1053:1080	superior adsorption capacity	1053:1080	BiOI-Ch exhibited superior adsorption capacity as compared to pure BiOI (33.78 mg g-1).
34993771	9	34	from	application	1790:1800	arg1	elimination					1809:1819	the elimination	1805:1819	the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1805:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	9	35	from	elimination	1809:1819	arg1	effluent					1878:1885	industrial effluent	1867:1885	industrial effluent after necessary optimization for large-scale operation	1867:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	7	36	theme	Freundlich	1318:1327	arg1	R2 = 0.99					1339:1347	R2 = 0.99	1339:1347	R2 = 0.99	1339:1347	The adsorption process obeyed pseudo-second-order kinetic model (R2 = 0.98) and was well fitted to Freundlich isotherm (R2 = 0.99).
34993771	7	36	theme	Freundlich	1318:1327	arg1	isotherm					1329:1336	Freundlich isotherm	1318:1336	Freundlich isotherm (R2 = 0.99)	1318:1348	The adsorption process obeyed pseudo-second-order kinetic model (R2 = 0.98) and was well fitted to Freundlich isotherm (R2 = 0.99).
34993771	9	37	theme	pollutants	1851:1860	arg1	elimination					1809:1819	the elimination	1805:1819	the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1805:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	9	38	from	effect	1709:1714	arg1	performance					1747:1757	improved performance	1738:1757	improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1738:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	6	39	dep	kinetic	1171:1177	arg1	models					1192:1197	models	1192:1197	models	1192:1197	To understand the dynamics of reaction, several kinetic and isotherm models were also examined.
34993771	8	40	theme	chitosan	1376:1383	arg1	addition					1355:1362	The addition	1351:1362	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication	1351:1453	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	9	41	theme	necessary	1893:1901	arg1	optimization					1903:1914	necessary optimization	1893:1914	necessary optimization for large-scale operation	1893:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	8	42	theme	adsorption	1582:1591	arg1	kinetics					1593:1600	fast adsorption kinetics	1577:1600	fast adsorption kinetics	1577:1600	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	0	43	theme	kinetic	131:137	arg1	investigations					167:180	kinetic, isotherm and thermodynamic investigations	131:180	kinetic, isotherm and thermodynamic investigations	131:180	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	8	44	theme	enhanced	1462:1469	arg1	nanocomposite					1520:1532	the nanocomposite	1516:1532	the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1516:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	8	44	theme	enhanced	1462:1469	arg1	groups					1482:1487	enhanced functional groups	1462:1487	enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1462:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	8	45	theme	biowaste	1367:1374	arg1	chitosan					1376:1383	biowaste chitosan	1367:1383	biowaste chitosan	1367:1383	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	2	46	theme	bismuth	416:422	arg1	BiOI-Ch					444:450	BiOI-Ch	444:450	BiOI-Ch	444:450	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	2	46	theme	bismuth	416:422	arg1	bionanocomposite					398:413	a novel bionanocomposite	390:413	a novel bionanocomposite	390:413	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	2	46	theme	bismuth	416:422	arg1	oxyiodide-chitosan					424:441	bismuth oxyiodide-chitosan	416:441	bismuth oxyiodide-chitosan (BiOI-Ch)	416:451	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	0	47	theme	ultrasonic	49:58	arg1	waves					60:64	ultrasonic waves	49:64	ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations	49:180	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	8	48	theme	improved	1606:1613	arg1	transfer					1620:1627	improved mass transfer	1606:1627	improved mass transfer	1606:1627	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	2	49	theme	adsorption	529:538	arg1	capacity					540:547	adsorption capacity	529:547	adsorption capacity	529:547	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	7	50	theme	pseudo-second-order	1249:1267	arg1	R2 = 0.98					1284:1292	R2 = 0.98	1284:1292	R2 = 0.98	1284:1292	The adsorption process obeyed pseudo-second-order kinetic model (R2 = 0.98) and was well fitted to Freundlich isotherm (R2 = 0.99).
34993771	7	50	theme	pseudo-second-order	1249:1267	arg1	model					1277:1281	pseudo-second-order kinetic model	1249:1281	pseudo-second-order kinetic model (R2 = 0.98)	1249:1293	The adsorption process obeyed pseudo-second-order kinetic model (R2 = 0.98) and was well fitted to Freundlich isotherm (R2 = 0.99).
34993771	8	51	theme	non-toxic	1388:1396	arg1	nanoparticles					1412:1424	non-toxic bismuth-based nanoparticles	1388:1424	non-toxic bismuth-based nanoparticles coupled with ultrasonication	1388:1453	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	4	52	theme	adsorption	980:989	arg1	81.01 mg g-1					1021:1032	81.01 mg g-1	1021:1032	81.01 mg g-1	1021:1032	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	4	52	theme	adsorption	980:989	arg1	capacity					991:998	the maximal adsorption capacity	968:998	the maximal adsorption capacity	968:998	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	0	53	theme	isotherm	140:147	arg1	investigations					167:180	kinetic, isotherm and thermodynamic investigations	131:180	kinetic, isotherm and thermodynamic investigations	131:180	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	4	54	theme	optimized	940:948	arg1	conditions					950:959	optimized conditions	940:959	optimized conditions	940:959	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	2	55	theme	ultrasonic	568:577	arg1	waves					579:583	ultrasonic waves	568:583	ultrasonic waves	568:583	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	4	56	from	solution	925:932	arg1	AMX					912:914	approximately 90% AMX	894:914	approximately 90% AMX from the solution	894:932	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	8	57	theme	adsorption	1556:1565	arg1	capacity					1567:1574	superior adsorption capacity	1547:1574	superior adsorption capacity	1547:1574	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	9	58	theme	improved	1738:1745	arg1	performance					1747:1757	improved performance	1738:1757	improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1738:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	8	59	theme	mass	1615:1618	arg1	transfer					1620:1627	improved mass transfer	1606:1627	improved mass transfer	1606:1627	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	0	60	theme	thermodynamic	153:165	arg1	investigations					167:180	kinetic, isotherm and thermodynamic investigations	131:180	kinetic, isotherm and thermodynamic investigations	131:180	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	3	61	theme	operational	669:679	arg1	concentration					784:796	80 mg g-1 AMX concentration	770:796	80 mg g-1 AMX concentration	770:796	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	61	theme	operational	669:679	arg1	temperature					825:835	temperature 298 K	825:841	temperature 298 K	825:841	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	61	theme	operational	669:679	arg1	pH					764:765	pH 3	764:767	pH 3	764:767	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	61	theme	operational	669:679	arg1	dose					819:822	1.7 g L-1 adsorbent dose	799:822	1.7 g L-1 adsorbent dose	799:822	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	61	theme	operational	669:679	arg1	20 min					868:873	ultrasonication time 20 min	847:873	ultrasonication time 20 min	847:873	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	61	theme	operational	669:679	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	8	62	theme	bismuth-based	1398:1410	arg1	nanoparticles					1412:1424	non-toxic bismuth-based nanoparticles	1388:1424	non-toxic bismuth-based nanoparticles coupled with ultrasonication	1388:1453	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	3	63	theme	Multiple	604:611	arg1	experiments					619:629	Multiple batch experiments	604:629	Multiple batch experiments	604:629	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	9	64	theme	novel	1762:1766	arg1	BiOI-Ch					1768:1774	novel BiOI-Ch	1762:1774	novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1762:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	0	65	from	medium	123:128	arg1	removal					87:93	adsorptive removal	76:93	adsorptive removal of amoxicillin from aqueous medium	76:128	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	3	66	theme	batch	613:617	arg1	experiments					619:629	Multiple batch experiments	604:629	Multiple batch experiments	604:629	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	4	67	dep	%	910:910	arg1	90					908:909	90	908:909	90	908:909	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	0	68	dep	investigations	167:180	arg1	removal					87:93	adsorptive removal	76:93	adsorptive removal of amoxicillin from aqueous medium	76:128	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	2	69	dep	method	496:501	arg1	capacity					540:547	adsorption capacity	529:547	adsorption capacity	529:547	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	5	70	theme	superior	1053:1060	arg1	capacity					1073:1080	superior adsorption capacity	1053:1080	superior adsorption capacity	1053:1080	BiOI-Ch exhibited superior adsorption capacity as compared to pure BiOI (33.78 mg g-1).
34993771	9	71	theme	persistent	1824:1833	arg1	pollutants					1851:1860	persistent and detrimental pollutants	1824:1860	persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1824:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	9	72	theme	industrial	1867:1876	arg1	effluent					1878:1885	industrial effluent	1867:1885	industrial effluent after necessary optimization for large-scale operation	1867:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	8	73	theme	nanocomposite	1520:1532	arg1	nanocomposite					1520:1532	the nanocomposite	1516:1532	the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1516:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	8	73	theme	nanocomposite	1520:1532	arg1	area					1508:1511	surface area	1500:1511	enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1462:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	8	73	theme	nanocomposite	1520:1532	arg1	groups					1482:1487	enhanced functional groups	1462:1487	enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules	1462:1660	The addition of biowaste chitosan to non-toxic bismuth-based nanoparticles coupled with ultrasonication led to enhanced functional groups as well as surface area of the nanocomposite resulting in superior adsorption capacity, fast adsorption kinetics and improved mass transfer for the removal of AMX molecules.
34993771	3	74	theme	optimum	661:667	arg1	concentration					784:796	80 mg g-1 AMX concentration	770:796	80 mg g-1 AMX concentration	770:796	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	74	theme	optimum	661:667	arg1	temperature					825:835	temperature 298 K	825:841	temperature 298 K	825:841	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	74	theme	optimum	661:667	arg1	pH					764:765	pH 3	764:767	pH 3	764:767	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	74	theme	optimum	661:667	arg1	dose					819:822	1.7 g L-1 adsorbent dose	799:822	1.7 g L-1 adsorbent dose	799:822	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	74	theme	optimum	661:667	arg1	20 min					868:873	ultrasonication time 20 min	847:873	ultrasonication time 20 min	847:873	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	74	theme	optimum	661:667	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	9	75	theme	potential	1780:1788	arg1	application					1790:1800	potential application	1780:1800	potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1780:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	2	76	theme	current	375:381	arg1	study					383:387	the current study	371:387	the current study	371:387	In the current study, a novel bionanocomposite, bismuth oxyiodide-chitosan (BiOI-Ch), was synthesized by a facile precipitation method and its amoxicillin (AMX) adsorption capacity in the presence of ultrasonic waves has been explored.
34993771	7	77	theme	adsorption	1223:1232	arg1	process					1234:1240	The adsorption process	1219:1240	The adsorption process	1219:1240	The adsorption process obeyed pseudo-second-order kinetic model (R2 = 0.98) and was well fitted to Freundlich isotherm (R2 = 0.99).
34993771	3	78	theme	80 mg g-1	770:778	arg1	concentration					784:796	80 mg g-1 AMX concentration	770:796	80 mg g-1 AMX concentration	770:796	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	78	theme	80 mg g-1	770:778	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	0	79	theme	adsorptive	76:85	arg1	removal					87:93	adsorptive removal	76:93	adsorptive removal of amoxicillin from aqueous medium	76:128	New bismuth oxyiodide/chitosan nanocomposite for ultrasonic waves expedited adsorptive removal of amoxicillin from aqueous medium: kinetic, isotherm and thermodynamic investigations.
34993771	4	80	theme	maximal	972:978	arg1	81.01 mg g-1					1021:1032	81.01 mg g-1	1021:1032	81.01 mg g-1	1021:1032	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	4	80	theme	maximal	972:978	arg1	capacity					991:998	the maximal adsorption capacity	968:998	the maximal adsorption capacity	968:998	Composite removed approximately 90% AMX from the solution under optimized conditions, while the maximal adsorption capacity was determined to be 81.01 mg g-1.
34993771	3	81	theme	AMX	780:782	arg1	concentration					784:796	80 mg g-1 AMX concentration	770:796	80 mg g-1 AMX concentration	770:796	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	3	81	theme	AMX	780:782	arg1	parameters					681:690	the optimum operational parameters	657:690	the optimum operational parameters for maximum adsorption of AMX	657:720	Multiple batch experiments were performed to achieve the optimum operational parameters for maximum adsorption of AMX and the obtained results were as follows: pH 3, 80 mg g-1 AMX concentration, 1.7 g L-1 adsorbent dose, temperature 298 K and ultrasonication time 20 min.
34993771	7	82	theme	kinetic	1269:1275	arg1	R2 = 0.98					1284:1292	R2 = 0.98	1284:1292	R2 = 0.98	1284:1292	The adsorption process obeyed pseudo-second-order kinetic model (R2 = 0.98) and was well fitted to Freundlich isotherm (R2 = 0.99).
34993771	7	82	theme	kinetic	1269:1275	arg1	model					1277:1281	pseudo-second-order kinetic model	1249:1281	pseudo-second-order kinetic model (R2 = 0.98)	1249:1293	The adsorption process obeyed pseudo-second-order kinetic model (R2 = 0.98) and was well fitted to Freundlich isotherm (R2 = 0.99).
34993771	9	83	theme	ultrasonication	1719:1733	arg1	effect					1709:1714	the synergistic effect	1693:1714	the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation	1693:1940	Thus, this study demonstrates the synergistic effect of ultrasonication in improved performance of novel BiOI-Ch for potential application in the elimination of persistent and detrimental pollutants from industrial effluent after necessary optimization for large-scale operation.
34993771	1	84	theme	harmful	244:250	arg1	effects					252:258	harmful effects	244:258	harmful effects	244:258	Amoxicillin (AMX) is a widely used antibiotic, which induces harmful effects to nature via bioaccumulation and persistence in the environment if discharged untreated into water bodies.
37068716	5	0	dep	microspheres	860:871	arg1	adhered					885:891	adhered	885:891	adhered	885:891	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	5	0	dep	microspheres	860:871	arg1	administration					784:797	intrarectal administration	772:797	intrarectal administration	772:797	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	1	1	theme	bowel	156:160	arg1	disease					162:168	inflammatory bowel disease	143:168	inflammatory bowel disease	143:168	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	4	2	theme	glucomannan	605:615	arg1	LDKT					650:653	LDKT	650:653	LDKT	650:653	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	2	theme	glucomannan	605:615	arg1	microspheres					636:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres	560:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm	560:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	7	3	theme	LDKT	1272:1275	arg1	microspheres					1277:1288	the LDKT microspheres	1268:1288	the LDKT microspheres	1268:1288	Overall, the LDKT microspheres are promising carriers to deliver drugs for UC treatment.
37068716	7	3	theme	LDKT	1272:1275	arg1	carriers					1304:1311	promising carriers	1294:1311	promising carriers	1294:1311	Overall, the LDKT microspheres are promising carriers to deliver drugs for UC treatment.
37068716	6	4	theme	ROS	1150:1152	arg1	cytokines					1175:1183	pro-inflammatory cytokines	1158:1183	pro-inflammatory cytokines	1158:1183	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	6	4	theme	ROS	1150:1152	arg1	levels					1132:1137	the levels	1128:1137	the levels of colonic ROS	1128:1152	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	1	5	theme	disease	162:168	arg1	type					135:138	an important type	122:138	an important type of inflammatory bowel disease	122:168	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	4	6	theme	conjugate/konjac	588:603	arg1	LDKT					650:653	LDKT	650:653	LDKT	650:653	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	6	theme	conjugate/konjac	588:603	arg1	microspheres					636:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres	560:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm	560:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	6	7	theme	colonic	1142:1148	arg1	ROS					1150:1152	colonic ROS	1142:1152	colonic ROS	1142:1152	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	3	8	theme	-loaded	388:394	arg1	microspheres					405:416	olsalazine (Olsa)-loaded hydrogel microspheres	371:416	olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa)	371:428	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	3	8	theme	-loaded	388:394	arg1	LDKT/Olsa					419:427	LDKT/Olsa	419:427	LDKT/Olsa	419:427	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	2	9	theme	delivery	295:302	arg1	insufficiency					273:285	the insufficiency	269:285	the insufficiency of drug delivery and retention	269:316	The UC treatment is challenged by the insufficiency of drug delivery and retention.
37068716	6	10	theme	gut	1195:1197	arg1	integrity					1215:1223	gut mucosal barrier integrity	1195:1223	gut mucosal barrier integrity	1195:1223	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	4	11	theme	hydrogel	627:634	arg1	LDKT					650:653	LDKT	650:653	LDKT	650:653	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	11	theme	hydrogel	627:634	arg1	microspheres					636:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres	560:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm	560:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	2	12	theme	drug	290:293	arg1	delivery					295:302	drug delivery	290:302	drug delivery	290:302	The UC treatment is challenged by the insufficiency of drug delivery and retention.
37068716	4	13	theme	composite	617:625	arg1	LDKT					650:653	LDKT	650:653	LDKT	650:653	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	13	theme	composite	617:625	arg1	microspheres					636:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres	560:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm	560:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	3	14	theme	intrarectal	344:354	arg1	formulation					356:366	an intrarectal formulation	341:366	an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa)	341:428	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	5	15	theme	mucosa	929:934	arg1	surface					905:911	cationic surface	896:911	cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects	896:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	3	16	theme	good	435:438	arg1	bio-adhesiveness					440:455	good bio-adhesiveness	435:455	good bio-adhesiveness	435:455	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	0	17	theme	targeted	58:65	arg1	therapy					86:92	targeted ulcerative colitis therapy	58:92	targeted ulcerative colitis therapy	58:92	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.
37068716	6	18	theme	mucosal	1199:1205	arg1	integrity					1215:1223	gut mucosal barrier integrity	1195:1223	gut mucosal barrier integrity	1195:1223	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	1	19	theme	Ulcerative	95:104	arg1	disease					183:189	a chronic disease	173:189	a chronic disease characterized by intestinal dyshomeostasis	173:232	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	1	19	theme	Ulcerative	95:104	arg1	UC					115:116	UC	115:116	UC	115:116	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	1	19	theme	Ulcerative	95:104	arg1	colitis					106:112	Ulcerative colitis	95:112	Ulcerative colitis (UC) as an important type of inflammatory bowel disease	95:168	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	5	20	theme	inflammatory	916:927	arg1	mucosa					929:934	inflammatory mucosa	916:934	inflammatory mucosa	916:934	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	5	21	theme	dopamine-functionalized	827:849	arg1	microspheres					860:871	the negatively charged and dopamine-functionalized hydrogel microspheres	800:871	the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects	800:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	5	22	theme	antibacterial	984:996	arg1	effects					1020:1026	antibacterial and anti-inflammatory effects	984:1026	antibacterial and anti-inflammatory effects	984:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	1	23	theme	chronic	175:181	arg1	disease					183:189	a chronic disease	173:189	a chronic disease characterized by intestinal dyshomeostasis	173:232	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	1	23	theme	chronic	175:181	arg1	colitis					106:112	Ulcerative colitis	95:112	Ulcerative colitis (UC) as an important type of inflammatory bowel disease	95:168	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	0	24	theme	Bio-adhesive	0:11	arg1	microspheres					41:52	Bio-adhesive and ROS-scavenging hydrogel microspheres	0:52	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.	0:93	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.
37068716	4	25	theme	Low	560:562	arg1	LDKT					650:653	LDKT	650:653	LDKT	650:653	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	25	theme	Low	560:562	arg1	microspheres					636:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres	560:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm	560:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	6	26	theme	barrier	1207:1213	arg1	integrity					1215:1223	gut mucosal barrier integrity	1195:1223	gut mucosal barrier integrity	1195:1223	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	0	27	theme	hydrogel	32:39	arg1	microspheres					41:52	Bio-adhesive and ROS-scavenging hydrogel microspheres	0:52	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.	0:93	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.
37068716	5	28	theme	hydrogel	851:858	arg1	microspheres					860:871	the negatively charged and dopamine-functionalized hydrogel microspheres	800:871	the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects	800:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	2	29	theme	retention	308:316	arg1	insufficiency					273:285	the insufficiency	269:285	the insufficiency of drug delivery and retention	269:316	The UC treatment is challenged by the insufficiency of drug delivery and retention.
37068716	3	30	theme	oxygen	470:475	arg1	ROS					486:488	ROS	486:488	ROS	486:488	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	3	30	theme	oxygen	470:475	arg1	species					477:483	reactive oxygen species	461:483	reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect	461:557	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	0	31	theme	ROS-scavenging	17:30	arg1	microspheres					41:52	Bio-adhesive and ROS-scavenging hydrogel microspheres	0:52	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.	0:93	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.
37068716	4	32	theme	pectin-dopamine	572:586	arg1	LDKT					650:653	LDKT	650:653	LDKT	650:653	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	32	theme	pectin-dopamine	572:586	arg1	microspheres					636:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres	560:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm	560:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	5	33	theme	releasing	956:964	arg1	Zn2+					966:969	Zn2+	966:969	Zn2+	966:969	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	4	34	theme	methoxy	564:570	arg1	LDKT					650:653	LDKT	650:653	LDKT	650:653	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	34	theme	methoxy	564:570	arg1	microspheres					636:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres	560:647	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm	560:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	5	35	theme	cationic	896:903	arg1	surface					905:911	cationic surface	896:911	cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects	896:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	1	36	theme	intestinal	208:217	arg1	dyshomeostasis					219:232	intestinal dyshomeostasis	208:232	intestinal dyshomeostasis	208:232	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	2	37	theme	UC	239:240	arg1	treatment					242:250	The UC treatment	235:250	The UC treatment	235:250	The UC treatment is challenged by the insufficiency of drug delivery and retention.
37068716	3	38	theme	reactive	461:468	arg1	ROS					486:488	ROS	486:488	ROS	486:488	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	3	38	theme	reactive	461:468	arg1	species					477:483	reactive oxygen species	461:483	reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect	461:557	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	3	39	theme	hydrogel	396:403	arg1	microspheres					405:416	olsalazine (Olsa)-loaded hydrogel microspheres	371:416	olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa)	371:428	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	3	39	theme	hydrogel	396:403	arg1	LDKT/Olsa					419:427	LDKT/Olsa	419:427	LDKT/Olsa	419:427	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	0	40	theme	colitis	78:84	arg1	therapy					86:92	targeted ulcerative colitis therapy	58:92	targeted ulcerative colitis therapy	58:92	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.
37068716	5	41	theme	Zn2+	966:969	arg1	surface					905:911	cationic surface	896:911	cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects	896:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	3	42	theme	microspheres	405:416	arg1	formulation					356:366	an intrarectal formulation	341:366	an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa)	341:428	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	4	43	with	microspheres	636:647	arg1	size					663:666	a size	661:666	a size ranging from 10 to 100 μm	661:692	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	6	44	theme	mouse	1073:1077	arg1	model					1082:1086	the dextran sodium sulfate (DSS)-induced mouse UC model	1032:1086	the dextran sodium sulfate (DSS)-induced mouse UC model	1032:1086	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	5	45	theme	charged	815:821	arg1	microspheres					860:871	the negatively charged and dopamine-functionalized hydrogel microspheres	800:871	the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects	800:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	4	46	dep	100	687:689	arg1	to					684:685	to	684:685	to	684:685	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	5	47	theme	intrarectal	772:782	arg1	administration					784:797	intrarectal administration	772:797	intrarectal administration	772:797	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	1	48	theme	important	125:133	arg1	type					135:138	an important type	122:138	an important type of inflammatory bowel disease	122:168	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	5	49	theme	anti-inflammatory	1002:1018	arg1	effects					1020:1026	antibacterial and anti-inflammatory effects	984:1026	antibacterial and anti-inflammatory effects	984:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	6	50	theme	UC	1079:1080	arg1	model					1082:1086	the dextran sodium sulfate (DSS)-induced mouse UC model	1032:1086	the dextran sodium sulfate (DSS)-induced mouse UC model	1032:1086	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	3	51	theme	therapeutic	540:550	arg1	effect					552:557	therapeutic effect	540:557	therapeutic effect	540:557	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	1	52	theme	inflammatory	143:154	arg1	disease					162:168	inflammatory bowel disease	143:168	inflammatory bowel disease	143:168	Ulcerative colitis (UC) as an important type of inflammatory bowel disease is a chronic disease characterized by intestinal dyshomeostasis.
37068716	4	53	theme	ROS-sensitive	726:738	arg1	crosslinkers					753:764	crosslinkers	753:764	crosslinkers	753:764	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	53	theme	ROS-sensitive	726:738	arg1	Zn2+					717:720	Zn2+	717:720	Zn2+	717:720	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	4	53	theme	ROS-sensitive	726:738	arg1	thioketal					740:748	ROS-sensitive thioketal	726:748	ROS-sensitive thioketal	726:748	Low methoxy pectin-dopamine conjugate/konjac glucomannan composite hydrogel microspheres (LDKT) with a size ranging from 10 to 100 μm were prepared by using Zn2+ and ROS-sensitive thioketal as crosslinkers.
37068716	5	54	theme	ROS	948:950	arg1	surface					905:911	cationic surface	896:911	cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects	896:1026	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	5	55	theme	scavenging	937:946	arg1	ROS					948:950	scavenging ROS	937:950	scavenging ROS	937:950	Upon intrarectal administration, the negatively charged and dopamine-functionalized hydrogel microspheres efficiently adhered to cationic surface of inflammatory mucosa, scavenging ROS and releasing Zn2+ and Olsa for antibacterial and anti-inflammatory effects.
37068716	6	56	theme	-induced	1064:1071	arg1	model					1082:1086	the dextran sodium sulfate (DSS)-induced mouse UC model	1032:1086	the dextran sodium sulfate (DSS)-induced mouse UC model	1032:1086	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	0	57	theme	ulcerative	67:76	arg1	therapy					86:92	targeted ulcerative colitis therapy	58:92	targeted ulcerative colitis therapy	58:92	Bio-adhesive and ROS-scavenging hydrogel microspheres for targeted ulcerative colitis therapy.
37068716	6	58	theme	pro-inflammatory	1158:1173	arg1	cytokines					1175:1183	pro-inflammatory cytokines	1158:1183	pro-inflammatory cytokines	1158:1183	In the dextran sodium sulfate (DSS)-induced mouse UC model, the microspheres significantly reduced the levels of colonic ROS and pro-inflammatory cytokines, improved gut mucosal barrier integrity, and remarkably relieved colitis.
37068716	3	59	theme	drug	521:524	arg1	retention					526:534	drug retention	521:534	drug retention	521:534	Herein, we fabricated an intrarectal formulation of olsalazine (Olsa)-loaded hydrogel microspheres (LDKT/Olsa) with good bio-adhesiveness and reactive oxygen species (ROS)-scavenging ability to enhance drug retention and therapeutic effect.
37068716	7	60	theme	promising	1294:1302	arg1	microspheres					1277:1288	the LDKT microspheres	1268:1288	the LDKT microspheres	1268:1288	Overall, the LDKT microspheres are promising carriers to deliver drugs for UC treatment.
37068716	7	60	theme	promising	1294:1302	arg1	carriers					1304:1311	promising carriers	1294:1311	promising carriers	1294:1311	Overall, the LDKT microspheres are promising carriers to deliver drugs for UC treatment.
36063681	8	0	theme	apple	1448:1452	arg1	juices					1454:1459	fermented apple juices	1438:1459	fermented apple juices	1438:1459	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	3	1	theme	fermentation	478:489	arg1	efficacy					491:498	the fermentation efficacy	474:498	the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae	474:559	In this study, the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae was focused.
36063681	9	2	theme	commercial	1702:1711	arg1	strains					1719:1725	commercial yeast strains	1702:1725	commercial yeast strains	1702:1725	This work provides very good information of commercial yeast strains for industrial pálinka production.
36063681	0	3	theme	yeast	81:85	arg1	strains					87:93	different commercial yeast strains	60:93	different commercial yeast strains of Saccharomyces cerevisiae	60:121	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	6	4	theme	volatile	1049:1056	arg1	compounds					1058:1066	volatile compounds	1049:1066	volatile compounds	1049:1066	The differences in the concentration and composition of volatile compounds were recorded.
36063681	5	5	theme	-9.43 v/v 	866:875	arg1	%					865:865	%	865:865	%	865:865	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	6	theme	Uvaferm	932:938	arg1	strains					924:930	strains Uvaferm Danstil A and Fermicru AR2	924:965	strains Uvaferm Danstil A and Fermicru AR2	924:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	6	theme	Uvaferm	932:938	arg1	A					948:948	Uvaferm Danstil A	932:948	Uvaferm Danstil A	932:948	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	0	7	theme	Saccharomyces	98:110	arg1	cerevisiae					112:121	Saccharomyces cerevisiae	98:121	Saccharomyces cerevisiae	98:121	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	3	8	theme	strains	525:531	arg1	efficacy					491:498	the fermentation efficacy	474:498	the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae	474:559	In this study, the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae was focused.
36063681	3	9	theme	cerevisiae	550:559	arg1	strains					525:531	nine commercial yeast strains	503:531	nine commercial yeast strains of Saccharomyces cerevisiae	503:559	In this study, the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae was focused.
36063681	8	10	theme	characteristic	1587:1600	arg1	indicators					1602:1611	characteristic indicators	1587:1611	characteristic indicators of samples fermented with Uvaferm Danstil A	1587:1655	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	11	theme	fusel	1476:1480	arg1	2-methyl-1-butanol					1513:1530	2-methyl-1-butanol	1513:1530	2-methyl-1-butanol	1513:1530	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	11	theme	fusel	1476:1480	arg1	2-methyl-1-propanol					1492:1510	2-methyl-1-propanol	1492:1510	2-methyl-1-propanol	1492:1510	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	11	theme	fusel	1476:1480	arg1	alcohols					1482:1489	total fusel alcohols	1470:1489	total fusel alcohols	1470:1489	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	11	theme	fusel	1476:1480	arg1	compounds					1572:1580	total volatile compounds	1557:1580	total volatile compounds	1557:1580	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	11	theme	fusel	1476:1480	arg1	3-methyl-1-butanol					1533:1550	3-methyl-1-butanol	1533:1550	3-methyl-1-butanol	1533:1550	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	12	theme	samples	1616:1622	arg1	indicators					1602:1611	characteristic indicators	1587:1611	characteristic indicators of samples fermented with Uvaferm Danstil A	1587:1655	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	4	13	dep	sugars	674:679	arg1	fructose					682:689	fructose	682:689	fructose	682:689	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	2	14	theme	oenological	329:339	arg1	purpose					341:347	oenological purpose	329:347	oenological purpose	329:347	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	8	15	theme	total	1470:1474	arg1	2-methyl-1-butanol					1513:1530	2-methyl-1-butanol	1513:1530	2-methyl-1-butanol	1513:1530	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	15	theme	total	1470:1474	arg1	2-methyl-1-propanol					1492:1510	2-methyl-1-propanol	1492:1510	2-methyl-1-propanol	1492:1510	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	15	theme	total	1470:1474	arg1	alcohols					1482:1489	total fusel alcohols	1470:1489	total fusel alcohols	1470:1489	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	15	theme	total	1470:1474	arg1	compounds					1572:1580	total volatile compounds	1557:1580	total volatile compounds	1557:1580	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	15	theme	total	1470:1474	arg1	3-methyl-1-butanol					1533:1550	3-methyl-1-butanol	1533:1550	3-methyl-1-butanol	1533:1550	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	7	16	theme	compounds	1120:1128	arg1	levels					1095:1100	The highest levels	1083:1100	The highest levels of total volatile compounds	1083:1128	The highest levels of total volatile compounds were observed in samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2.
36063681	3	17	theme	commercial	508:517	arg1	strains					525:531	nine commercial yeast strains	503:531	nine commercial yeast strains of Saccharomyces cerevisiae	503:559	In this study, the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae was focused.
36063681	0	18	theme	cerevisiae	112:121	arg1	strains					87:93	different commercial yeast strains	60:93	different commercial yeast strains of Saccharomyces cerevisiae	60:121	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	2	19	dep	type	427:430	arg1	the					423:425	the	423:425	the	423:425	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	7	20	located	observed	1135:1142	arg1	samples					1147:1153	samples	1147:1153	samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2	1147:1239	The highest levels of total volatile compounds were observed in samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2.
36063681	7	20	located	observed	1135:1142	arg2	levels					1095:1100	The highest levels	1083:1100	The highest levels of total volatile compounds	1083:1128	The highest levels of total volatile compounds were observed in samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2.
36063681	3	21	theme	yeast	519:523	arg1	strains					525:531	nine commercial yeast strains	503:531	nine commercial yeast strains of Saccharomyces cerevisiae	503:559	In this study, the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae was focused.
36063681	5	22	theme	significant	824:834	arg1	capacity					797:804	the alcohol production capacity	774:804	the alcohol production capacity	774:804	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	22	theme	significant	824:834	arg1	differences					836:846	no significant differences	821:846	no significant differences	821:846	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	9	23	theme	industrial	1731:1740	arg1	production					1750:1759	industrial pálinka production	1731:1759	industrial pálinka production	1731:1759	This work provides very good information of commercial yeast strains for industrial pálinka production.
36063681	0	24	theme	Chemical	0:7	arg1	composition					22:32	Chemical and volatile composition	0:32	composition	22:32	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	6	25	from	differences	997:1007	arg1	composition					1034:1044	composition	1034:1044	composition	1034:1044	The differences in the concentration and composition of volatile compounds were recorded.
36063681	6	25	from	differences	997:1007	arg1	concentration					1016:1028	concentration	1016:1028	concentration	1016:1028	The differences in the concentration and composition of volatile compounds were recorded.
36063681	4	26	dep	fructose	682:689	arg1	sucrose					704:710	sucrose	704:710	sucrose	704:710	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	4	26	dep	fructose	682:689	arg1	glucose					692:698	glucose	692:698	glucose	692:698	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	8	27	theme	key	1393:1395	arg1	compounds					1267:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	27	theme	key	1393:1395	arg1	parameters					1397:1406	key parameters	1393:1406	key parameters for describing the profile of fermented apple juices	1393:1459	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	5	28	theme	alcohol	778:784	arg1	capacity					797:804	the alcohol production capacity	774:804	the alcohol production capacity	774:804	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	28	theme	alcohol	778:784	arg1	differences					836:846	no significant differences	821:846	no significant differences	821:846	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	8	29	theme	total	1252:1256	arg1	compounds					1267:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	29	theme	total	1252:1256	arg1	parameters					1397:1406	key parameters	1393:1406	key parameters for describing the profile of fermented apple juices	1393:1459	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	29	theme	total	1252:1256	arg1	2-methyl-1-propanol					1278:1296	2-methyl-1-propanol	1278:1296	2-methyl-1-propanol	1278:1296	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	29	theme	total	1252:1256	arg1	alcohols					1332:1339	total higher alcohols	1319:1339	total higher alcohols	1319:1339	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	29	theme	total	1252:1256	arg1	esters					1367:1372	total esters	1361:1372	total esters	1361:1372	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	29	theme	total	1252:1256	arg1	3-methyl-1-butanol					1299:1316	3-methyl-1-butanol	1299:1316	3-methyl-1-butanol	1299:1316	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	29	theme	total	1252:1256	arg1	acetate					1348:1354	ethyl acetate	1342:1354	ethyl acetate	1342:1354	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	30	theme	Meanwhile	1242:1250	arg1	compounds					1267:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	30	theme	Meanwhile	1242:1250	arg1	parameters					1397:1406	key parameters	1393:1406	key parameters for describing the profile of fermented apple juices	1393:1459	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	30	theme	Meanwhile	1242:1250	arg1	2-methyl-1-propanol					1278:1296	2-methyl-1-propanol	1278:1296	2-methyl-1-propanol	1278:1296	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	30	theme	Meanwhile	1242:1250	arg1	alcohols					1332:1339	total higher alcohols	1319:1339	total higher alcohols	1319:1339	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	30	theme	Meanwhile	1242:1250	arg1	esters					1367:1372	total esters	1361:1372	total esters	1361:1372	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	30	theme	Meanwhile	1242:1250	arg1	3-methyl-1-butanol					1299:1316	3-methyl-1-butanol	1299:1316	3-methyl-1-butanol	1299:1316	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	30	theme	Meanwhile	1242:1250	arg1	acetate					1348:1354	ethyl acetate	1342:1354	ethyl acetate	1342:1354	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	3	31	theme	Saccharomyces	536:548	arg1	cerevisiae					550:559	Saccharomyces cerevisiae	536:559	Saccharomyces cerevisiae	536:559	In this study, the fermentation efficacy of nine commercial yeast strains of Saccharomyces cerevisiae was focused.
36063681	5	32	theme	sugar	903:907	arg1	consumption					909:919	sugar consumption	903:919	sugar consumption of strains Uvaferm Danstil A and Fermicru AR2	903:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	33	theme	consumption	909:919	arg1	stronger					971:978	stronger	971:978	stronger	971:978	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	33	theme	consumption	909:919	arg1	ability					892:898	the ability	888:898	the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2	888:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	0	34	theme	volatile	13:20	arg1	composition					22:32	Chemical and volatile composition	0:32	composition	22:32	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	5	35	theme	strains	924:930	arg1	consumption					909:919	sugar consumption	903:919	sugar consumption of strains Uvaferm Danstil A and Fermicru AR2	903:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	2	36	theme	pálinka	304:310	arg1	production					290:299	the production	286:299	the production of pálinka	286:310	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	4	37	theme	simple	667:672	arg1	sugars					674:679	the simple sugars	663:679	the simple sugars (fructose, glucose and sucrose)	663:711	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	0	38	theme	Pálinka	37:43	arg1	composition					22:32	Chemical and volatile composition	0:32	composition	22:32	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	4	39	theme	juice	634:638	arg1	fermentation					612:623	alcoholic fermentation	602:623	alcoholic fermentation of apple juice	602:638	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	5	40	dep	differences	836:846	arg1	%					876:876	9.17 v/v %-9.43 v/v %	856:876	9.17 v/v %-9.43 v/v %	856:876	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	40	dep	differences	836:846	arg1	whereas					880:886	whereas	880:886	whereas	880:886	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	1	41	theme	applied	215:221	arg1	strain					229:234	applied yeast strain	215:234	applied yeast strain	215:234	Pálinka is Hungarian traditional alcoholic drink, and its quality is strongly depending on applied yeast strain.
36063681	7	42	theme	total	1105:1109	arg1	compounds					1120:1128	total volatile compounds	1105:1128	total volatile compounds	1105:1128	The highest levels of total volatile compounds were observed in samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2.
36063681	8	43	theme	volatile	1258:1265	arg1	compounds					1267:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	43	theme	volatile	1258:1265	arg1	parameters					1397:1406	key parameters	1393:1406	key parameters for describing the profile of fermented apple juices	1393:1459	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	43	theme	volatile	1258:1265	arg1	2-methyl-1-propanol					1278:1296	2-methyl-1-propanol	1278:1296	2-methyl-1-propanol	1278:1296	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	43	theme	volatile	1258:1265	arg1	alcohols					1332:1339	total higher alcohols	1319:1339	total higher alcohols	1319:1339	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	43	theme	volatile	1258:1265	arg1	esters					1367:1372	total esters	1361:1372	total esters	1361:1372	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	43	theme	volatile	1258:1265	arg1	3-methyl-1-butanol					1299:1316	3-methyl-1-butanol	1299:1316	3-methyl-1-butanol	1299:1316	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	43	theme	volatile	1258:1265	arg1	acetate					1348:1354	ethyl acetate	1342:1354	ethyl acetate	1342:1354	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	7	44	theme	volatile	1111:1118	arg1	compounds					1120:1128	total volatile compounds	1105:1128	total volatile compounds	1105:1128	The highest levels of total volatile compounds were observed in samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2.
36063681	1	45	theme	yeast	223:227	arg1	strain					229:234	applied yeast strain	215:234	applied yeast strain	215:234	Pálinka is Hungarian traditional alcoholic drink, and its quality is strongly depending on applied yeast strain.
36063681	7	46	theme	highest	1087:1093	arg1	levels					1095:1100	The highest levels	1083:1100	The highest levels of total volatile compounds	1083:1128	The highest levels of total volatile compounds were observed in samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2.
36063681	5	47	theme	production	786:795	arg1	capacity					797:804	the alcohol production capacity	774:804	the alcohol production capacity	774:804	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	47	theme	production	786:795	arg1	differences					836:846	no significant differences	821:846	no significant differences	821:846	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	9	48	theme	pálinka	1742:1748	arg1	production					1750:1759	industrial pálinka production	1731:1759	industrial pálinka production	1731:1759	This work provides very good information of commercial yeast strains for industrial pálinka production.
36063681	8	49	theme	total	1361:1365	arg1	compounds					1267:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	49	theme	total	1361:1365	arg1	esters					1367:1372	total esters	1361:1372	total esters	1361:1372	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	6	50	theme	compounds	1058:1066	arg1	composition					1034:1044	composition	1034:1044	composition	1034:1044	The differences in the concentration and composition of volatile compounds were recorded.
36063681	6	50	theme	compounds	1058:1066	arg1	concentration					1016:1028	concentration	1016:1028	concentration	1016:1028	The differences in the concentration and composition of volatile compounds were recorded.
36063681	5	51	dep	strains	924:930	arg1	strains					924:930	strains Uvaferm Danstil A and Fermicru AR2	924:965	strains Uvaferm Danstil A and Fermicru AR2	924:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	51	dep	strains	924:930	arg1	A					948:948	Uvaferm Danstil A	932:948	Uvaferm Danstil A	932:948	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	51	dep	strains	924:930	arg1	AR2					963:965	Fermicru AR2	954:965	Fermicru AR2	954:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	2	52	theme	fruit	447:451	arg1	quality					436:442	quality	436:442	quality	436:442	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	2	52	theme	fruit	447:451	arg1	type					427:430	type	427:430	type	427:430	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	8	53	theme	total	1557:1561	arg1	alcohols					1482:1489	total fusel alcohols	1470:1489	total fusel alcohols	1470:1489	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	53	theme	total	1557:1561	arg1	compounds					1572:1580	total volatile compounds	1557:1580	total volatile compounds	1557:1580	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	9	54	theme	yeast	1713:1717	arg1	strains					1719:1725	commercial yeast strains	1702:1725	commercial yeast strains	1702:1725	This work provides very good information of commercial yeast strains for industrial pálinka production.
36063681	6	55	dep	concentration	1016:1028	arg1	the					1012:1014	the	1012:1014	the	1012:1014	The differences in the concentration and composition of volatile compounds were recorded.
36063681	8	56	theme	Uvaferm	1639:1645	arg1	A					1655:1655	Uvaferm Danstil A	1639:1655	Uvaferm Danstil A	1639:1655	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	7	57	theme	Vin-O-Ferm	1206:1215	arg1	Roses					1217:1221	Vin-O-Ferm Roses	1206:1221	Vin-O-Ferm Roses	1206:1221	The highest levels of total volatile compounds were observed in samples fermented with Uvaferm Danstil A, Fermiblanc Arom, Vin-O-Ferm Roses, and Fermicru AR2.
36063681	9	58	theme	strains	1719:1725	arg1	information					1687:1697	very good information	1677:1697	very good information of commercial yeast strains for industrial pálinka production	1677:1759	This work provides very good information of commercial yeast strains for industrial pálinka production.
36063681	5	59	theme	Danstil	940:946	arg1	strains					924:930	strains Uvaferm Danstil A and Fermicru AR2	924:965	strains Uvaferm Danstil A and Fermicru AR2	924:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	59	theme	Danstil	940:946	arg1	A					948:948	Uvaferm Danstil A	932:948	Uvaferm Danstil A	932:948	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	4	60	theme	fermentation	749:760	arg1	end					742:744	the end	738:744	the end of fermentation	738:760	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	1	61	theme	Hungarian	135:143	arg1	drink					167:171	Hungarian traditional alcoholic drink	135:171	Hungarian traditional alcoholic drink	135:171	Pálinka is Hungarian traditional alcoholic drink, and its quality is strongly depending on applied yeast strain.
36063681	8	62	theme	ethyl	1342:1346	arg1	compounds					1267:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	62	theme	ethyl	1342:1346	arg1	acetate					1348:1354	ethyl acetate	1342:1354	ethyl acetate	1342:1354	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	63	theme	total	1319:1323	arg1	alcohols					1332:1339	total higher alcohols	1319:1339	total higher alcohols	1319:1339	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	63	theme	total	1319:1323	arg1	compounds					1267:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds	1242:1275	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	2	64	dep	efficacy	363:370	arg1	the					359:361	the	359:361	the	359:361	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	8	65	theme	fermented	1438:1446	arg1	juices					1454:1459	fermented apple juices	1438:1459	fermented apple juices	1438:1459	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	2	66	theme	yeast	267:271	arg1	strains					273:279	all commercial yeast strains	252:279	all commercial yeast strains used the production of pálinka	252:310	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	1	67	theme	traditional	145:155	arg1	drink					167:171	Hungarian traditional alcoholic drink	135:171	Hungarian traditional alcoholic drink	135:171	Pálinka is Hungarian traditional alcoholic drink, and its quality is strongly depending on applied yeast strain.
36063681	8	68	theme	volatile	1563:1570	arg1	alcohols					1482:1489	total fusel alcohols	1470:1489	total fusel alcohols	1470:1489	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	8	68	theme	volatile	1563:1570	arg1	compounds					1572:1580	total volatile compounds	1557:1580	total volatile compounds	1557:1580	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	0	69	theme	commercial	70:79	arg1	strains					87:93	different commercial yeast strains	60:93	different commercial yeast strains of Saccharomyces cerevisiae	60:121	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	4	70	theme	apple	628:632	arg1	juice					634:638	apple juice	628:638	apple juice	628:638	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	2	71	theme	commercial	256:265	arg1	strains					273:279	all commercial yeast strains	252:279	all commercial yeast strains used the production of pálinka	252:310	Unfortunately, all commercial yeast strains used the production of pálinka are selected for oenological purpose, and thus the efficacy and aroma releasing capacity are vary depending on the type and quality of fruit used.
36063681	8	72	dep	total	1319:1323	arg1	higher					1325:1330	higher	1325:1330	higher	1325:1330	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	1	73	theme	alcoholic	157:165	arg1	drink					167:171	Hungarian traditional alcoholic drink	135:171	Hungarian traditional alcoholic drink	135:171	Pálinka is Hungarian traditional alcoholic drink, and its quality is strongly depending on applied yeast strain.
36063681	8	74	theme	Danstil	1647:1653	arg1	A					1655:1655	Uvaferm Danstil A	1639:1655	Uvaferm Danstil A	1639:1655	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	4	75	theme	alcoholic	602:610	arg1	fermentation					612:623	alcoholic fermentation	602:623	alcoholic fermentation of apple juice	602:638	All strains were able to do alcoholic fermentation of apple juice quite efficiently, and the simple sugars (fructose, glucose and sucrose) were almost exhausted at the end of fermentation.
36063681	0	76	theme	different	60:68	arg1	strains					87:93	different commercial yeast strains	60:93	different commercial yeast strains of Saccharomyces cerevisiae	60:121	Chemical and volatile composition of Pálinka produced using different commercial yeast strains of Saccharomyces cerevisiae.
36063681	8	77	theme	juices	1454:1459	arg1	profile					1427:1433	the profile	1423:1433	the profile of fermented apple juices	1423:1459	Meanwhile total volatile compounds, 2-methyl-1-propanol, 3-methyl-1-butanol, total higher alcohols, ethyl acetate, and total esters were considered as key parameters for describing the profile of fermented apple juices, whereas total fusel alcohols, 2-methyl-1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol, and total volatile compounds were characteristic indicators of samples fermented with Uvaferm Danstil A.
36063681	5	78	theme	Fermicru	954:961	arg1	strains					924:930	strains Uvaferm Danstil A and Fermicru AR2	924:965	strains Uvaferm Danstil A and Fermicru AR2	924:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	78	theme	Fermicru	954:961	arg1	AR2					963:965	Fermicru AR2	954:965	Fermicru AR2	954:965	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	5	79	theme	9.17 v/v 	856:864	arg1	%					865:865	%	865:865	%	865:865	Meanwhile, the alcohol production capacity and yield were no significant differences (around 9.17 v/v %-9.43 v/v %), whereas the ability of sugar consumption of strains Uvaferm Danstil A and Fermicru AR2 was stronger than others.
36063681	9	80	theme	good	1682:1685	arg1	information					1687:1697	very good information	1677:1697	very good information of commercial yeast strains for industrial pálinka production	1677:1759	This work provides very good information of commercial yeast strains for industrial pálinka production.
35763420	5	0	theme	Genome	676:681	arg1	sequencing					683:692	Genome sequencing	676:692	Genome sequencing	676:692	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	4	1	theme	gene	428:431	arg1	sequences					433:441	16S rRNA gene sequences	419:441	16S rRNA gene sequences	419:441	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	10	2	theme	phenotypic	1237:1246	arg1	data					1267:1270	phylogenetic and phenotypic and chemotaxonomic data	1220:1270	phylogenetic and phenotypic and chemotaxonomic data	1220:1270	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	6	3	with	values	805:810	arg1	LC6T					857:860	A. alkaliphilus LC6T	841:860	A. alkaliphilus LC6T	841:860	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	6	3	with	values	805:810	arg1	TGAT					835:838	A. methylotrophus TGAT	817:838	A. methylotrophus TGAT	817:838	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	6	3	with	values	805:810	arg1	CCM1646T					877:884	A. ramosus CCM1646T	866:884	A. ramosus CCM1646T	866:884	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	6	4	dep	A.	817:818	arg1	methylotrophus					820:833	methylotrophus	820:833	methylotrophus	820:833	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	7	5	dep	anteiso-C15 	954:965	arg1	 0					984:985	 0	984:985	 0	984:985	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	7	5	dep	anteiso-C15 	954:965	arg1	iso-C16 					991:998	iso-C16 	991:998	iso-C16 	991:998	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	7	5	dep	anteiso-C15 	954:965	arg1	 0					1000:1001	 0	1000:1001	 0	1000:1001	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	7	5	dep	anteiso-C15 	954:965	arg1	 0					967:968	 0	967:968	 0	967:968	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	2	6	theme	novel	96:100	arg1	bacterium					182:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	94:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T	94:208	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	10	7	theme	Arthrobacter	1327:1338	arg1	species					1306:1312	a novel species	1298:1312	a novel species	1298:1312	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	10	8	theme	cavernae	1373:1380	arg1	sp					1382:1383	the name Arthrobacter cavernae sp	1351:1383	the name Arthrobacter cavernae sp	1351:1383	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	6	9	dep	A.	841:842	arg1	alkaliphilus					844:855	alkaliphilus	844:855	alkaliphilus	844:855	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	3	10	theme	R2A	335:337	arg1	agar					339:342	R2A agar	335:342	R2A agar	335:342	The isolate grew optimally on R2A agar at 25 °C, pH 8.0 and with 0.5 % (w/v) NaCl.
35763420	9	11	theme	unidentified	1184:1195	arg1	lipids					1197:1202	unidentified lipids	1184:1202	unidentified lipids	1184:1202	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylinositol and unidentified lipids.
35763420	10	12	theme	genus	1321:1325	arg1	Arthrobacter					1327:1338	the genus Arthrobacter	1317:1338	the genus Arthrobacter	1317:1338	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	10	13	theme	chemotaxonomic	1252:1265	arg1	data					1267:1270	phylogenetic and phenotypic and chemotaxonomic data	1220:1270	phylogenetic and phenotypic and chemotaxonomic data	1220:1270	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	2	14	attach	isolated	214:221	arg1	sediment					228:235	sediment	228:235	sediment of karst cave collected in Libo county, Guizhou Province, PR China	228:302	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	2	14	attach	isolated	214:221	arg2	bacterium					182:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	94:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T	94:208	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	8	15	theme	major	1008:1012	arg1	MK-9					1038:1041	MK-9	1038:1041	MK-9	1038:1041	The major respiratory quinone was MK-9(H2).
35763420	8	15	theme	major	1008:1012	arg1	quinone					1026:1032	The major respiratory quinone	1004:1032	The major respiratory quinone	1004:1032	The major respiratory quinone was MK-9(H2).
35763420	2	16	theme	non-endospore-forming	133:153	arg1	bacterium					182:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	94:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T	94:208	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	10	17	theme	phylogenetic	1220:1231	arg1	data					1267:1270	phylogenetic and phenotypic and chemotaxonomic data	1220:1270	phylogenetic and phenotypic and chemotaxonomic data	1220:1270	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	10	18	theme	strain	1273:1278	arg1	PO-11T					1280:1285	strain PO-11T	1273:1285	strain PO-11T	1273:1285	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	6	19	theme	relatedness	793:803	arg1	values					805:810	Its DNA-DNA relatedness values	781:810	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T	781:884	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	10	20	theme	name	1355:1358	arg1	sp					1382:1383	the name Arthrobacter cavernae sp	1351:1383	the name Arthrobacter cavernae sp	1351:1383	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	5	21	theme	DNA	749:751	arg1	content					757:763	the genomic DNA G+C content	737:763	the genomic DNA G+C content	737:763	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	5	21	theme	DNA	749:751	arg1	%					778:778	66.16 mol%	769:778	66.16 mol%	769:778	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	4	22	theme	alkaliphilus	605:616	arg1	LC6T					618:621	Arthrobacter alkaliphilus LC6T	592:621	Arthrobacter alkaliphilus LC6T (97.7 %)	592:630	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	22	theme	alkaliphilus	605:616	arg1	%					629:629	97.7 %	624:629	97.7 %	624:629	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	2	23	theme	aerobic	124:130	arg1	bacterium					182:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	94:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T	94:208	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	12	24	theme	type	1408:1411	arg1	strain					1413:1418	The type strain	1404:1418	The type strain	1404:1418	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	12	24	theme	type	1408:1411	arg1	PO-11T					1430:1435	strain PO-11T	1423:1435	strain PO-11T (=CCTCC AB 2021070T=LMG 32459T)	1423:1467	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	5	25	theme	4 073 119 bp	720:731	arg1	size					712:715	a genome size	703:715	a genome size of 4 073 119 bp	703:731	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	5	26	theme	66.16 mol	769:777	arg1	content					757:763	the genomic DNA G+C content	737:763	the genomic DNA G+C content	737:763	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	5	26	theme	66.16 mol	769:777	arg1	%					778:778	66.16 mol%	769:778	66.16 mol%	769:778	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	0	27	theme	cavernae	13:20	arg1	sp					22:23	Arthrobacter cavernae sp	0:23	Arthrobacter cavernae sp.	0:24	Arthrobacter cavernae sp.
35763420	4	28	theme	16S	419:421	arg1	sequences					433:441	16S rRNA gene sequences	419:441	16S rRNA gene sequences	419:441	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	3	29	theme	%	374:374	arg1	NaCl					382:385	0.5 % (w/v) NaCl	370:385	0.5 % (w/v) NaCl	370:385	The isolate grew optimally on R2A agar at 25 °C, pH 8.0 and with 0.5 % (w/v) NaCl.
35763420	0	30	theme	Arthrobacter	0:11	arg1	sp					22:23	Arthrobacter cavernae sp	0:23	Arthrobacter cavernae sp.	0:24	Arthrobacter cavernae sp.
35763420	1	31	attach	isolated	56:63	arg2	actinobacterium					40:54	a novel actinobacterium	32:54	a novel actinobacterium isolated from sediment of karst cave	32:91	nov., a novel actinobacterium isolated from sediment of karst cave.
35763420	1	31	attach	isolated	56:63	arg1	sediment					70:77	sediment	70:77	sediment of karst cave	70:91	nov., a novel actinobacterium isolated from sediment of karst cave.
35763420	9	32	theme	polar	1052:1056	arg1	lipids					1058:1063	The polar lipids	1048:1063	The polar lipids	1048:1063	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylinositol and unidentified lipids.
35763420	1	33	theme	karst	82:86	arg1	cave					88:91	karst cave	82:91	karst cave	82:91	nov., a novel actinobacterium isolated from sediment of karst cave.
35763420	4	34	theme	Arthrobacter	592:603	arg1	LC6T					618:621	Arthrobacter alkaliphilus LC6T	592:621	Arthrobacter alkaliphilus LC6T (97.7 %)	592:630	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	34	theme	Arthrobacter	592:603	arg1	%					629:629	97.7 %	624:629	97.7 %	624:629	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	35	theme	genus	478:482	arg1	Arthrobacter					484:495	the genus Arthrobacter	474:495	the genus Arthrobacter	474:495	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	6	36	dep	A.	866:867	arg1	ramosus					869:875	ramosus	869:875	ramosus	869:875	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	1	37	theme	cave	88:91	arg1	sediment					70:77	sediment	70:77	sediment of karst cave	70:91	nov., a novel actinobacterium isolated from sediment of karst cave.
35763420	4	38	theme	%	568:568	arg1	similarity					579:588	98.3 % sequence similarity	563:588	98.3 % sequence similarity	563:588	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	38	theme	%	568:568	arg1	TGAT					557:560	Arthrobacter methylotrophus TGAT	529:560	Arthrobacter methylotrophus TGAT (98.3 % sequence similarity)	529:589	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	2	39	theme	PR	295:296	arg1	China					298:302	PR China	295:302	PR China	295:302	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	6	40	theme	A.	866:867	arg1	CCM1646T					877:884	A. ramosus CCM1646T	866:884	A. ramosus CCM1646T	866:884	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	2	41	theme	rod-shaped	171:180	arg1	bacterium					182:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	94:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T	94:208	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	4	42	theme	98.3 	563:567	arg1	%					568:568	%	568:568	%	568:568	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	6	43	theme	DNA-DNA	785:791	arg1	relatedness					793:803	Its DNA-DNA relatedness	781:803	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T	781:884	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	7	44	theme	fatty	937:941	arg1	acids					943:947	The main fatty acids	928:947	The main fatty acids	928:947	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	7	44	theme	fatty	937:941	arg1	anteiso-C15 					954:965	anteiso-C15 	954:965	anteiso-C15 	954:965	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	3	45	dep	%	374:374	arg1	w/v					377:379	w/v	377:379	w/v	377:379	The isolate grew optimally on R2A agar at 25 °C, pH 8.0 and with 0.5 % (w/v) NaCl.
35763420	5	46	theme	G+C	753:755	arg1	content					757:763	the genomic DNA G+C content	737:763	the genomic DNA G+C content	737:763	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	5	46	theme	G+C	753:755	arg1	%					778:778	66.16 mol%	769:778	66.16 mol%	769:778	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	3	47	theme	0.5 	370:373	arg1	%					374:374	%	374:374	%	374:374	The isolate grew optimally on R2A agar at 25 °C, pH 8.0 and with 0.5 % (w/v) NaCl.
35763420	10	48	theme	Arthrobacter	1360:1371	arg1	sp					1382:1383	the name Arthrobacter cavernae sp	1351:1383	the name Arthrobacter cavernae sp	1351:1383	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	2	49	theme	non-motile	156:165	arg1	bacterium					182:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	94:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T	94:208	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	12	50	theme	=CCTCC AB	1438:1446	arg1	PO-11T					1430:1435	strain PO-11T	1423:1435	strain PO-11T (=CCTCC AB 2021070T=LMG 32459T)	1423:1467	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	12	50	theme	=CCTCC AB	1438:1446	arg1	32459T					1461:1466	=CCTCC AB 2021070T=LMG 32459T	1438:1466	=CCTCC AB 2021070T=LMG 32459T	1438:1466	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	4	51	theme	sequence	570:577	arg1	similarity					579:588	98.3 % sequence similarity	563:588	98.3 % sequence similarity	563:588	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	51	theme	sequence	570:577	arg1	TGAT					557:560	Arthrobacter methylotrophus TGAT	529:560	Arthrobacter methylotrophus TGAT (98.3 % sequence similarity)	529:589	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	5	52	theme	genome	705:710	arg1	size					712:715	a genome size	703:715	a genome size of 4 073 119 bp	703:731	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	8	53	theme	respiratory	1014:1024	arg1	MK-9					1038:1041	MK-9	1038:1041	MK-9	1038:1041	The major respiratory quinone was MK-9(H2).
35763420	8	53	theme	respiratory	1014:1024	arg1	quinone					1026:1032	The major respiratory quinone	1004:1032	The major respiratory quinone	1004:1032	The major respiratory quinone was MK-9(H2).
35763420	4	54	theme	Phylogenetic	388:399	arg1	analysis					401:408	Phylogenetic analysis	388:408	Phylogenetic analysis based on 16S rRNA gene sequences	388:441	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	55	theme	rRNA	423:426	arg1	sequences					433:441	16S rRNA gene sequences	419:441	16S rRNA gene sequences	419:441	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	6	56	theme	A.	841:842	arg1	LC6T					857:860	A. alkaliphilus LC6T	841:860	A. alkaliphilus LC6T	841:860	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	7	57	theme	main	932:935	arg1	acids					943:947	The main fatty acids	928:947	The main fatty acids	928:947	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	7	57	theme	main	932:935	arg1	anteiso-C15 					954:965	anteiso-C15 	954:965	anteiso-C15 	954:965	The main fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
35763420	6	58	theme	A.	817:818	arg1	TGAT					835:838	A. methylotrophus TGAT	817:838	A. methylotrophus TGAT	817:838	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	10	59	theme	novel	1300:1304	arg1	species					1306:1312	a novel species	1298:1312	a novel species	1298:1312	Thus, based on phylogenetic and phenotypic and chemotaxonomic data, strain PO-11T represents a novel species of the genus Arthrobacter, for which the name Arthrobacter cavernae sp.
35763420	2	60	dep	Province	285:292	arg1	China					298:302	PR China	295:302	PR China	295:302	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	1	61	theme	novel	34:38	arg1	actinobacterium					40:54	a novel actinobacterium	32:54	a novel actinobacterium isolated from sediment of karst cave	32:91	nov., a novel actinobacterium isolated from sediment of karst cave.
35763420	1	62	dep	actinobacterium	40:54	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel actinobacterium isolated from sediment of karst cave.
35763420	12	63	theme	strain	1423:1428	arg1	strain					1413:1418	The type strain	1404:1418	The type strain	1404:1418	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	12	63	theme	strain	1423:1428	arg1	PO-11T					1430:1435	strain PO-11T	1423:1435	strain PO-11T (=CCTCC AB 2021070T=LMG 32459T)	1423:1467	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	12	63	theme	strain	1423:1428	arg1	32459T					1461:1466	=CCTCC AB 2021070T=LMG 32459T	1438:1466	=CCTCC AB 2021070T=LMG 32459T	1438:1466	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	4	64	theme	Arthrobacter	636:647	arg1	CCM1646T					657:664	Arthrobacter ramosus CCM1646T	636:664	Arthrobacter ramosus CCM1646T (97.1 %)	636:673	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	64	theme	Arthrobacter	636:647	arg1	%					672:672	97.1 %	667:672	97.1 %	667:672	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	65	dep	Arthrobacter	636:647	arg1	ramosus					649:655	ramosus	649:655	ramosus	649:655	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	66	theme	Arthrobacter	529:540	arg1	similarity					579:588	98.3 % sequence similarity	563:588	98.3 % sequence similarity	563:588	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	66	theme	Arthrobacter	529:540	arg1	TGAT					557:560	Arthrobacter methylotrophus TGAT	529:560	Arthrobacter methylotrophus TGAT (98.3 % sequence similarity)	529:589	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	4	67	dep	Arthrobacter	529:540	arg1	methylotrophus					542:555	methylotrophus	542:555	methylotrophus	542:555	Phylogenetic analysis based on 16S rRNA gene sequences showed that PO-11T belonged to the genus Arthrobacter and was most closely related to Arthrobacter methylotrophus TGAT (98.3 % sequence similarity), Arthrobacter alkaliphilus LC6T (97.7 %) and Arthrobacter ramosus CCM1646T (97.1 %).
35763420	2	68	theme	cave	246:249	arg1	sediment					228:235	sediment	228:235	sediment of karst cave collected in Libo county, Guizhou Province, PR China	228:302	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	5	69	theme	genomic	741:747	arg1	content					757:763	the genomic DNA G+C content	737:763	the genomic DNA G+C content	737:763	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	5	69	theme	genomic	741:747	arg1	%					778:778	66.16 mol%	769:778	66.16 mol%	769:778	Genome sequencing revealed a genome size of 4 073 119 bp and the genomic DNA G+C content was 66.16 mol%.
35763420	6	70	theme	23.2 	906:910	arg1	%					911:911	23.0, 22.9 and 23.2 %	891:911	%	911:911	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	6	71	theme	23.0	891:894	arg1	%					911:911	23.0, 22.9 and 23.2 %	891:911	%	911:911	Its DNA-DNA relatedness values with A. methylotrophus TGAT, A. alkaliphilus LC6T and A. ramosus CCM1646T were 23.0, 22.9 and 23.2 %, respectively.
35763420	2	72	theme	Gram-stain-positive	103:121	arg1	bacterium					182:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	94:190	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T	94:208	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	2	73	theme	karst	240:244	arg1	cave					246:249	karst cave	240:249	karst cave collected in Libo county, Guizhou Province, PR China	240:302	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated PO-11T was isolated from sediment of karst cave collected in Libo county, Guizhou Province, PR China.
35763420	12	74	theme	2021070T=LMG	1448:1459	arg1	PO-11T					1430:1435	strain PO-11T	1423:1435	strain PO-11T (=CCTCC AB 2021070T=LMG 32459T)	1423:1467	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35763420	12	74	theme	2021070T=LMG	1448:1459	arg1	32459T					1461:1466	=CCTCC AB 2021070T=LMG 32459T	1438:1466	=CCTCC AB 2021070T=LMG 32459T	1438:1466	The type strain is strain PO-11T (=CCTCC AB 2021070T=LMG 32459T).
35259431	0	0	theme	Stability	95:103	arg1	studies					115:121	Stability & kinetic studies	95:121	chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies	37:121	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	7	1	theme	9th	1368:1370	arg1	cycle					1381:1385	the 9th repeated cycle	1364:1385	the 9th repeated cycle of utilization	1364:1400	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	7	2	theme	utilization	1390:1400	arg1	cycle					1381:1385	the 9th repeated cycle	1364:1385	the 9th repeated cycle of utilization	1364:1400	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	3	3	theme	surface	325:331	arg1	morphology					333:342	surface morphology	325:342	surface morphology	325:342	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	3	theme	surface	325:331	arg1	Size					308:311	Size	308:311	Size	308:311	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	8	4	theme	enzymatic	1449:1457	arg1	activity					1459:1466	enzymatic activity	1449:1466	enzymatic activity	1449:1466	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	5	5	theme	catalytic	962:970	arg1	activity					972:979	the optimum catalytic activity	950:979	the optimum catalytic activity	950:979	Immobilized & solubilized trypsin exhibited the optimum catalytic activity at pH 8.5, 60 °C, and pH 7.8, 45 °C respectively.
35259431	5	6	theme	&	918:918	arg1	trypsin					932:938	Immobilized & solubilized trypsin	906:938	Immobilized & solubilized trypsin	906:938	Immobilized & solubilized trypsin exhibited the optimum catalytic activity at pH 8.5, 60 °C, and pH 7.8, 45 °C respectively.
35259431	0	7	theme	kinetic	107:113	arg1	studies					115:121	Stability & kinetic studies	95:121	chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies	37:121	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	7	8	theme	&	1313:1313	arg1	2 h					1295:1297	2 h	1295:1297	2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization	1295:1400	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	4	9	theme	maximum	851:857	arg1	activity					869:876	the maximum expressed activity	847:876	the maximum expressed activity of the immobilized trypsin	847:903	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	1	10	dep	Try	133:135	arg1	EC					138:139	EC	138:139	EC	138:139	Trypsin (Try, EC.
35259431	7	11	theme	catalytic	1339:1347	arg1	activity					1349:1356	catalytic activity	1339:1356	catalytic activity	1339:1356	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	0	12	theme	&	105:105	arg1	studies					115:121	Stability & kinetic studies	95:121	chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies	37:121	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	6	13	theme	free	1102:1105	arg1	trypsin					1107:1113	immobilized (27.12 μM, 8.82 μM/min) & free trypsin	1064:1113	trypsin	1107:1113	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	5	14	theme	optimum	954:960	arg1	activity					972:979	the optimum catalytic activity	950:979	the optimum catalytic activity	950:979	Immobilized & solubilized trypsin exhibited the optimum catalytic activity at pH 8.5, 60 °C, and pH 7.8, 45 °C respectively.
35259431	8	15	theme	promising	1578:1586	arg1	ZnO/Chitosan-GA-Trypsin					1543:1565	ZnO/Chitosan-GA-Trypsin	1543:1565	ZnO/Chitosan-GA-Trypsin	1543:1565	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	8	15	theme	promising	1578:1586	arg1	biocatalyst					1588:1598	a promising biocatalyst	1576:1598	a promising biocatalyst for large-scale biotechnological applications	1576:1644	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	2	16	theme	Schiff-base	286:296	arg1	linkages					298:305	Schiff-base linkages	286:305	Schiff-base linkages	286:305	3.4.21.4) was effectively immobilized on the surface of glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite through covalent attachment via Schiff-base linkages.
35259431	7	17	theme	activity	1349:1356	arg1	activity					1349:1356	catalytic activity	1339:1356	catalytic activity	1339:1356	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	7	17	theme	activity	1349:1356	arg1	%					1334:1334	nearly 50%	1325:1334	nearly 50% of catalytic activity	1325:1356	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	8	18	theme	activity	1459:1466	arg1	%					1444:1444	50%	1442:1444	50% of enzymatic activity	1442:1466	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	8	18	theme	activity	1459:1466	arg1	activity					1459:1466	enzymatic activity	1449:1466	enzymatic activity	1449:1466	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	5	19	theme	solubilized	920:930	arg1	trypsin					932:938	Immobilized & solubilized trypsin	906:938	Immobilized & solubilized trypsin	906:938	Immobilized & solubilized trypsin exhibited the optimum catalytic activity at pH 8.5, 60 °C, and pH 7.8, 45 °C respectively.
35259431	7	20	theme	initial	1262:1268	arg1	activity					1270:1277	initial activity	1262:1277	initial activity	1262:1277	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	1	21	dep	Trypsin	124:130	arg1	Try					133:135	Try	133:135	Try	133:135	Trypsin (Try, EC.
35259431	7	22	theme	activity	1270:1277	arg1	%					1257:1257	67%	1255:1257	67% of initial activity	1255:1277	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	7	22	theme	activity	1270:1277	arg1	activity					1270:1277	initial activity	1262:1277	initial activity	1262:1277	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	3	23	theme	percentage	347:356	arg1	composition					368:378	percentage elemental composition	347:378	percentage elemental composition of the prepared ZnO nanoparticles	347:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	8	24	dep	4 °C.	1496:1500	arg1	suggest					1530:1536	suggest	1530:1536	suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications	1530:1644	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	0	25	theme	Zinc	0:3	arg1	oxide					5:9	Zinc oxide	0:9	Zinc oxide	0:9	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	6	26	dep	parameters	1039:1048	arg1	Vmax					1055:1058	Vmax	1055:1058	Vmax	1055:1058	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	6	26	dep	parameters	1039:1048	arg1	Km					1051:1052	Km	1051:1052	Km	1051:1052	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	3	27	theme	prepared	387:394	arg1	nanoparticles					400:412	the prepared ZnO nanoparticles	383:412	the prepared ZnO nanoparticles	383:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	6	28	theme	trypsin	1107:1113	arg1	parameters					1039:1048	Kinetic parameters	1031:1048	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min)	1031:1137	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	3	29	theme	electron	588:595	arg1	TEM					609:611	TEM	609:611	TEM	609:611	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	29	theme	electron	588:595	arg1	microscopy					597:606	transmission electron microscopy	575:606	transmission electron microscopy (TEM)	575:612	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	7	30	theme	Immobilized	1225:1235	arg1	trypsin					1237:1243	Immobilized trypsin	1225:1243	Immobilized trypsin	1225:1243	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	6	31	dep	trypsin	1107:1113	arg1	25.76 μM					1116:1123	25.76 μM	1116:1123	25.76 μM	1116:1123	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	6	31	dep	trypsin	1107:1113	arg1	4.16 μM/min					1126:1136	4.16 μM/min	1126:1136	4.16 μM/min	1126:1136	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	6	31	dep	trypsin	1107:1113	arg1	8.82 μM/min					1087:1097	immobilized (27.12 μM, 8.82 μM/min) & free trypsin	1064:1113	8.82 μM/min	1087:1097	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	3	32	theme	ZnO	396:398	arg1	nanoparticles					400:412	the prepared ZnO nanoparticles	383:412	the prepared ZnO nanoparticles	383:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	7	33	theme	repeated	1372:1379	arg1	cycle					1381:1385	the 9th repeated cycle	1364:1385	the 9th repeated cycle of utilization	1364:1400	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	6	34	theme	trypsin	1186:1192	arg1	efficiency					1172:1181	efficiency	1172:1181	efficiency of trypsin	1172:1192	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	4	35	theme	incubation	747:756	arg1	16 h					764:767	16 h	764:767	16 h	764:767	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	4	35	theme	incubation	747:756	arg1	time					758:761	incubation time	747:761	incubation time (16 h)	747:768	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	0	36	theme	chitosan	37:44	arg1	surfaces					46:53	chitosan surfaces	37:53	chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies	37:121	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	3	37	theme	Scanning	615:622	arg1	SEM					645:647	SEM	645:647	SEM	645:647	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	37	theme	Scanning	615:622	arg1	microscopy					633:642	Scanning electron microscopy	615:642	Scanning electron microscopy (SEM)	615:648	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	4	38	theme	trypsin	897:903	arg1	activity					869:876	the maximum expressed activity	847:876	the maximum expressed activity of the immobilized trypsin	847:903	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	6	39	theme	&	1100:1100	arg1	trypsin					1107:1113	immobilized (27.12 μM, 8.82 μM/min) & free trypsin	1064:1113	trypsin	1107:1113	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	6	40	theme	Kinetic	1031:1037	arg1	parameters					1039:1048	Kinetic parameters	1031:1048	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min)	1031:1137	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	4	41	dep	conditions	735:744	arg1	1.8 mg/ml					793:801	1.8 mg/ml	793:801	1.8 mg/ml	793:801	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	4	41	dep	conditions	735:744	arg1	pH					809:810	pH (7.8)	809:816	pH (7.8)	809:816	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	4	41	dep	conditions	735:744	arg1	16 h					764:767	16 h	764:767	16 h	764:767	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	4	41	dep	conditions	735:744	arg1	time					758:761	incubation time	747:761	incubation time (16 h)	747:768	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	4	41	dep	conditions	735:744	arg1	concentration					778:790	enzyme concentration	771:790	enzyme concentration (1.8 mg/ml)	771:802	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	4	42	theme	immobilized	885:895	arg1	trypsin					897:903	the immobilized trypsin	881:903	the immobilized trypsin	881:903	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	3	43	theme	transmission	575:586	arg1	TEM					609:611	TEM	609:611	TEM	609:611	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	43	theme	transmission	575:586	arg1	microscopy					597:606	transmission electron microscopy	575:606	transmission electron microscopy (TEM)	575:612	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	44	theme	Fourier-transform	493:509	arg1	FTIR					534:537	FTIR	534:537	FTIR	534:537	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	44	theme	Fourier-transform	493:509	arg1	spectroscopy					520:531	Fourier-transform infrared spectroscopy	493:531	Fourier-transform infrared spectroscopy (FTIR)	493:538	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	45	theme	chitosan-coated	418:432	arg1	nanocomposite					438:450	chitosan-coated ZnO nanocomposite	418:450	chitosan-coated ZnO nanocomposite	418:450	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	45	theme	chitosan-coated	418:432	arg1	nanoparticles					400:412	the prepared ZnO nanoparticles	383:412	the prepared ZnO nanoparticles	383:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	2	46	theme	-activated	216:225	arg1	nanocomposite					240:252	glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite	198:252	glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite	198:252	3.4.21.4) was effectively immobilized on the surface of glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite through covalent attachment via Schiff-base linkages.
35259431	3	47	theme	UV-Visible	468:477	arg1	spectroscopy					479:490	UV-Visible spectroscopy	468:490	UV-Visible spectroscopy	468:490	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	48	theme	ZnO	434:436	arg1	nanocomposite					438:450	chitosan-coated ZnO nanocomposite	418:450	chitosan-coated ZnO nanocomposite	418:450	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	48	theme	ZnO	434:436	arg1	nanoparticles					400:412	the prepared ZnO nanoparticles	383:412	the prepared ZnO nanoparticles	383:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	8	49	theme	current	1513:1519	arg1	findings					1521:1528	the current findings	1509:1528	the current findings	1509:1528	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	3	50	theme	nanoparticles	400:412	arg1	nanocomposite					438:450	chitosan-coated ZnO nanocomposite	418:450	chitosan-coated ZnO nanocomposite	418:450	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	50	theme	nanoparticles	400:412	arg1	nanoparticles					400:412	the prepared ZnO nanoparticles	383:412	the prepared ZnO nanoparticles	383:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	50	theme	nanoparticles	400:412	arg1	composition					368:378	percentage elemental composition	347:378	percentage elemental composition of the prepared ZnO nanoparticles	347:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	51	theme	elemental	358:366	arg1	composition					368:378	percentage elemental composition	347:378	percentage elemental composition of the prepared ZnO nanoparticles	347:412	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	52	dep	&	345:345	arg1	studied					457:463	studied	457:463	were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques	452:709	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	52	dep	&	345:345	arg1	Size					308:311	Size	308:311	Size	308:311	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	52	dep	&	345:345	arg1	morphology					333:342	surface morphology	325:342	surface morphology	325:342	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	52	dep	&	345:345	arg1	structure					314:322	structure	314:322	structure	314:322	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	8	53	theme	storage	1485:1491	arg1	90 days					1474:1480	90 days	1474:1480	90 days of storage	1474:1491	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	6	54	theme	immobilized	1064:1074	arg1	trypsin					1107:1113	immobilized (27.12 μM, 8.82 μM/min) & free trypsin	1064:1113	trypsin	1107:1113	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	4	55	theme	immobilization	720:733	arg1	conditions					735:744	Optimal immobilization conditions	712:744	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8))	712:817	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	0	56	theme	covalent	59:66	arg1	immobilization					68:81	covalent immobilization	59:81	covalent immobilization of trypsin	59:92	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	4	57	theme	expressed	859:867	arg1	activity					869:876	the maximum expressed activity	847:876	the maximum expressed activity of the immobilized trypsin	847:903	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	8	58	theme	immobilized	1413:1423	arg1	trypsin					1425:1431	immobilized trypsin	1413:1431	immobilized trypsin	1413:1431	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	0	59	dep	surfaces	46:53	arg1	studies					115:121	Stability & kinetic studies	95:121	chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies	37:121	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	4	60	theme	Optimal	712:718	arg1	conditions					735:744	Optimal immobilization conditions	712:744	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8))	712:817	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	8	61	theme	large-scale	1604:1614	arg1	applications					1633:1644	large-scale biotechnological applications	1604:1644	large-scale biotechnological applications	1604:1644	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	3	62	theme	electron	624:631	arg1	SEM					645:647	SEM	645:647	SEM	645:647	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	62	theme	electron	624:631	arg1	microscopy					633:642	Scanning electron microscopy	615:642	Scanning electron microscopy (SEM)	615:648	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	7	63	theme	incubation	1302:1311	arg1	&					1313:1313	incubation &	1302:1313	incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization	1302:1400	Immobilized trypsin preserved 67% of initial activity at 50 °C during 2 h of incubation & sustained nearly 50% of catalytic activity until the 9th repeated cycle of utilization.
35259431	2	64	theme	covalent	262:269	arg1	attachment					271:280	covalent attachment	262:280	covalent attachment via Schiff-base linkages	262:305	3.4.21.4) was effectively immobilized on the surface of glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite through covalent attachment via Schiff-base linkages.
35259431	3	65	theme	infrared	511:518	arg1	FTIR					534:537	FTIR	534:537	FTIR	534:537	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	65	theme	infrared	511:518	arg1	spectroscopy					520:531	Fourier-transform infrared spectroscopy	493:531	Fourier-transform infrared spectroscopy (FTIR)	493:538	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	4	66	theme	enzyme	771:776	arg1	1.8 mg/ml					793:801	1.8 mg/ml	793:801	1.8 mg/ml	793:801	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	4	66	theme	enzyme	771:776	arg1	concentration					778:790	enzyme concentration	771:790	enzyme concentration (1.8 mg/ml)	771:802	Optimal immobilization conditions (incubation time (16 h), enzyme concentration (1.8 mg/ml), and pH (7.8)) were investigated to obtain the maximum expressed activity of the immobilized trypsin.
35259431	3	67	theme	Energy-Dispersive	655:671	arg1	EDAX					694:697	EDAX	694:697	EDAX	694:697	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	67	theme	Energy-Dispersive	655:671	arg1	Microanalysis					679:691	Energy-Dispersive X-Ray Microanalysis	655:691	Energy-Dispersive X-Ray Microanalysis (EDAX)	655:698	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	68	dep	spectroscopy	479:490	arg1	techniques					700:709	techniques	700:709	techniques	700:709	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	5	69	theme	Immobilized	906:916	arg1	trypsin					932:938	Immobilized & solubilized trypsin	906:938	Immobilized & solubilized trypsin	906:938	Immobilized & solubilized trypsin exhibited the optimum catalytic activity at pH 8.5, 60 °C, and pH 7.8, 45 °C respectively.
35259431	6	70	theme	27.12 μM	1077:1084	arg1	trypsin					1107:1113	immobilized (27.12 μM, 8.82 μM/min) & free trypsin	1064:1113	trypsin	1107:1113	Kinetic parameters (Km, Vmax) of immobilized (27.12 μM, 8.82 μM/min) & free trypsin (25.76 μM, 4.16 μM/min) were determined, indicating that efficiency of trypsin improves after immobilization.
35259431	0	71	theme	trypsin	86:92	arg1	immobilization					68:81	covalent immobilization	59:81	covalent immobilization of trypsin	59:92	Zinc oxide nanoparticles-impregnated chitosan surfaces for covalent immobilization of trypsin: Stability & kinetic studies.
35259431	3	72	theme	X-Ray	673:677	arg1	EDAX					694:697	EDAX	694:697	EDAX	694:697	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	72	theme	X-Ray	673:677	arg1	Microanalysis					679:691	Energy-Dispersive X-Ray Microanalysis	655:691	Energy-Dispersive X-Ray Microanalysis (EDAX)	655:698	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	2	73	theme	nanocomposite	240:252	arg1	surface					187:193	the surface	183:193	the surface of glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite	183:252	3.4.21.4) was effectively immobilized on the surface of glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite through covalent attachment via Schiff-base linkages.
35259431	3	74	theme	X-Ray	541:545	arg1	XRD					569:571	XRD	569:571	XRD	569:571	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	74	theme	X-Ray	541:545	arg1	analysis					559:566	X-Ray diffraction analysis	541:566	X-Ray diffraction analysis (XRD)	541:572	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	8	75	theme	biotechnological	1616:1631	arg1	applications					1633:1644	large-scale biotechnological applications	1604:1644	large-scale biotechnological applications	1604:1644	Moreover, immobilized trypsin retained 50% of enzymatic activity after 90 days of storage at 4 °C. Hence, the current findings suggest that ZnO/Chitosan-GA-Trypsin would be a promising biocatalyst for large-scale biotechnological applications.
35259431	2	76	theme	ZnO/Chitosan	227:238	arg1	nanocomposite					240:252	glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite	198:252	glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite	198:252	3.4.21.4) was effectively immobilized on the surface of glutaraldehyde(GA)-activated ZnO/Chitosan nanocomposite through covalent attachment via Schiff-base linkages.
35259431	3	77	theme	diffraction	547:557	arg1	XRD					569:571	XRD	569:571	XRD	569:571	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
35259431	3	77	theme	diffraction	547:557	arg1	analysis					559:566	X-Ray diffraction analysis	541:566	X-Ray diffraction analysis (XRD)	541:572	Size, structure, surface morphology, & percentage elemental composition of the prepared ZnO nanoparticles and chitosan-coated ZnO nanocomposite were studied by UV-Visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-Ray diffraction analysis (XRD), transmission electron microscopy (TEM), Scanning electron microscopy (SEM), and Energy-Dispersive X-Ray Microanalysis (EDAX) techniques.
36738944	8	0	from	mechanisms	1190:1199	arg1	mice					1228:1231	DSS-induced UC mice	1213:1231	DSS-induced UC mice	1213:1231	Ultimately, the potential mechanisms of EHLHP on DSS-induced UC mice were verified.
36738944	3	1	theme	experiment	652:661	arg1	verification					663:674	the animal experiment verification	641:674	the animal experiment verification	641:674	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	2	theme	potential	502:510	arg1	mechanisms					520:529	the potential anti-UC mechanisms	498:529	the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP)	498:576	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	6	3	theme	sequencing	917:926	arg1	method					928:933	the transcriptomics sequencing method	897:933	the transcriptomics sequencing method	897:933	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	9	4	dep	RESULTS	1248:1254	arg1	total					1258:1262	A total	1256:1262	RESULTS A total of 34 components of EHLHP	1248:1288	RESULTS A total of 34 components of EHLHP were identified by UPLC-QE-MS analysis.
36738944	11	5	theme	MAPK	1612:1615	arg1	pathway					1627:1633	MAPK signaling pathway	1612:1633	MAPK signaling pathway	1612:1633	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	5	theme	MAPK	1612:1615	arg1	pathway					1603:1609	PI3K-Akt signaling pathway	1584:1609	PI3K-Akt signaling pathway	1584:1609	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	3	6	theme	ethanol	538:544	arg1	extraction					546:555	50% ethanol extraction	534:555	50% ethanol extraction of HL	534:561	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	17	7	theme	viable	2600:2605	arg1	strategy					2607:2614	a viable strategy	2598:2614	a viable strategy to elucidate the mechanisms of TCM classical formula	2598:2667	It would provide a viable strategy to elucidate the mechanisms of TCM classical formula.
36738944	3	8	theme	50	534:535	arg1	%					536:536	%	536:536	%	536:536	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	1	9	theme	classical	203:211	arg1	prescription					213:224	a classical prescription	201:224	a classical prescription	201:224	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	9	theme	classical	203:211	arg1	Decoction					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction	136:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP)	136:198	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	8	10	theme	potential	1180:1188	arg1	mechanisms					1190:1199	the potential mechanisms	1176:1199	the potential mechanisms of EHLHP on DSS-induced UC mice	1176:1231	Ultimately, the potential mechanisms of EHLHP on DSS-induced UC mice were verified.
36738944	16	11	theme	potential	2378:2386	arg1	mechanisms					2388:2397	The potential mechanisms	2374:2397	The potential mechanisms of HLHP extract on DSS-induced UC mice	2374:2436	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	15	12	from	expression	2168:2177	arg1	determination					2251:2263	F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination	2214:2263	F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination	2214:2263	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	2	13	from	treatment	383:391	arg1	constituents					329:340	effective constituents	319:340	effective constituents	319:340	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	2	13	from	treatment	383:391	arg1	mechanisms					357:366	underlying mechanisms	346:366	underlying mechanisms	346:366	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	16	14	theme	integrated	2474:2483	arg1	analysis					2485:2492	integrated analysis	2474:2492	integrated analysis of transcriptomes and network pharmacology	2474:2535	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	14	15	theme	mice	2136:2139	arg1	barrier					2122:2128	the intestinal barrier	2107:2128	the intestinal barrier of UC mice	2107:2139	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	14	16	theme	staining	2043:2050	arg1	results					2052:2058	The IHC staining results	2035:2058	The IHC staining results of ZO-1, Occludin and Claudin-1	2035:2090	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	13	17	theme	level	2028:2032	arg1	decrease					1989:1996	the decrease	1985:1996	the decrease of DAI value and inflammation level	1985:2032	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	8	18	theme	UC	1225:1226	arg1	mice					1228:1231	DSS-induced UC mice	1213:1231	DSS-induced UC mice	1213:1231	Ultimately, the potential mechanisms of EHLHP on DSS-induced UC mice were verified.
36738944	7	19	theme	key	1040:1042	arg1	targets					1044:1050	The key targets	1036:1050	The key targets	1036:1050	The key targets and pathways of EHLHP were selected by the combination of the network pharmacology and transcriptomics results.
36738944	1	20	theme	RELEVANCE	157:165	arg1	prescription					213:224	a classical prescription	201:224	a classical prescription	201:224	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	20	theme	RELEVANCE	157:165	arg1	HLHP					194:197	HLHP	194:197	HLHP	194:197	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	20	theme	RELEVANCE	157:165	arg1	Decoction					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction	136:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP)	136:198	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	13	21	theme	DAI	2001:2003	arg1	value					2005:2009	DAI value	2001:2009	DAI value	2001:2009	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	15	22	theme	macrophage	2329:2338	arg1	M1					2353:2354	macrophage polarization M1	2329:2354	macrophage polarization M1 to M2	2329:2360	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	16	23	theme	DSS-induced	2418:2428	arg1	mice					2433:2436	DSS-induced UC mice	2418:2436	DSS-induced UC mice	2418:2436	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	16	24	theme	analysis	2485:2492	arg1	prediction					2460:2469	the prediction	2456:2469	the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification	2456:2578	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	0	25	theme	intestinal	69:78	arg1	barrier					88:94	intestinal mucosal barrier	69:94	intestinal mucosal barrier	69:94	Huanglian-Houpo extract attenuates DSS-induced UC mice by protecting intestinal mucosal barrier and regulating macrophage polarization.
36738944	9	26	theme	34	1267:1268	arg1	components					1270:1279	34 components	1267:1279	34 components of EHLHP	1267:1288	RESULTS A total of 34 components of EHLHP were identified by UPLC-QE-MS analysis.
36738944	12	27	theme	cell	1832:1835	arg1	receptor					1837:1844	B cell receptor	1830:1844	B cell receptor	1830:1844	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	12	27	theme	cell	1832:1835	arg1	IL-17					1823:1827	IL-17	1823:1827	IL-17	1823:1827	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	12	28	theme	calcium	1714:1720	arg1	pathway					1732:1738	calcium signaling pathway	1714:1738	calcium signaling pathway	1714:1738	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	3	29	dep	aimed	482:486	arg1	AIM					454:456	AIM	454:456	AIM OF THE STUDY	454:469	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	6	30	from	EHLHP	1006:1010	arg1	treatment					1019:1027	the treatment	1015:1027	the treatment of UC	1015:1033	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	16	31	from	mechanisms	2388:2397	arg1	mice					2433:2436	DSS-induced UC mice	2418:2436	DSS-induced UC mice	2418:2436	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	4	32	theme	chemical	714:721	arg1	composition					723:733	the chemical composition	710:733	the chemical composition of EHLHP	710:742	METHODS Primarily, we identified the chemical composition of EHLHP via UPLC-QE-MS analysis.
36738944	2	33	theme	colitis	407:413	arg1	treatment					383:391	the treatment	379:391	the treatment of ulcerative colitis (UC)	379:418	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	0	34	theme	Huanglian-Houpo	0:14	arg1	extract					16:22	Huanglian-Houpo extract	0:22	Huanglian-Houpo extract	0:22	Huanglian-Houpo extract attenuates DSS-induced UC mice by protecting intestinal mucosal barrier and regulating macrophage polarization.
36738944	16	35	dep	CONCLUSION	2363:2372	arg1	revealed					2443:2450	revealed	2443:2450	revealed	2443:2450	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	3	36	theme	STUDY	465:469	arg1	AIM					454:456	AIM	454:456	AIM OF THE STUDY	454:469	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	5	37	theme	UC	829:830	arg1	treatment					832:840	UC treatment	829:840	UC treatment	829:840	A visualization network with components-targets-pathways on UC treatment were constructed using network pharmacology.
36738944	17	38	theme	classical	2651:2659	arg1	formula					2661:2667	TCM classical formula	2647:2667	TCM classical formula	2647:2667	It would provide a viable strategy to elucidate the mechanisms of TCM classical formula.
36738944	14	39	theme	UC	2133:2134	arg1	mice					2136:2139	UC mice	2133:2139	UC mice	2133:2139	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	7	40	theme	transcriptomics	1139:1153	arg1	combination					1095:1105	the combination	1091:1105	the combination of the network pharmacology and transcriptomics results	1091:1161	The key targets and pathways of EHLHP were selected by the combination of the network pharmacology and transcriptomics results.
36738944	0	41	theme	UC	47:48	arg1	mice					50:53	DSS-induced UC mice	35:53	DSS-induced UC mice	35:53	Huanglian-Houpo extract attenuates DSS-induced UC mice by protecting intestinal mucosal barrier and regulating macrophage polarization.
36738944	6	42	theme	UC	1032:1033	arg1	treatment					1019:1027	the treatment	1015:1027	the treatment of UC	1015:1033	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	2	43	theme	HLHP	371:374	arg1	constituents					329:340	effective constituents	319:340	effective constituents	319:340	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	2	43	theme	HLHP	371:374	arg1	mechanisms					357:366	underlying mechanisms	346:366	underlying mechanisms	346:366	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	7	44	theme	pharmacology	1122:1133	arg1	combination					1095:1105	the combination	1091:1105	the combination of the network pharmacology and transcriptomics results	1091:1161	The key targets and pathways of EHLHP were selected by the combination of the network pharmacology and transcriptomics results.
36738944	16	45	theme	HLHP	2402:2405	arg1	extract					2407:2413	HLHP extract	2402:2413	HLHP extract	2402:2413	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	16	46	theme	pharmacology	2524:2535	arg1	analysis					2485:2492	integrated analysis	2474:2492	integrated analysis of transcriptomes and network pharmacology	2474:2535	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	16	46	theme	pharmacology	2524:2535	arg1	verification					2567:2578	animal test verification	2555:2578	animal test verification	2555:2578	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	15	47	theme	F4/80+	2214:2219	arg1	determination					2251:2263	F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination	2214:2263	F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination	2214:2263	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	3	48	theme	animal	645:650	arg1	experiment					652:661	the animal experiment	641:661	the animal experiment verification	641:674	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	13	49	theme	induced	1902:1908	arg1	mice					1913:1916	DSS induced UC mice	1898:1916	DSS induced UC mice	1898:1916	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	3	50	theme	anti-UC	512:518	arg1	mechanisms					520:529	the potential anti-UC mechanisms	498:529	the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP)	498:576	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	15	51	theme	immune	2198:2203	arg1	cells					2205:2209	immune cells	2198:2209	immune cells	2198:2209	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	5	52	theme	visualization	771:783	arg1	network					785:791	A visualization network	769:791	A visualization network with components-targets-pathways on UC treatment	769:840	A visualization network with components-targets-pathways on UC treatment were constructed using network pharmacology.
36738944	12	53	theme	inflammatory	1741:1752	arg1	pathway					1764:1770	inflammatory signaling pathway	1741:1770	inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG)	1741:1798	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	16	54	theme	animal	2555:2560	arg1	verification					2567:2578	animal test verification	2555:2578	animal test verification	2555:2578	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	14	55	theme	intestinal	2111:2120	arg1	barrier					2122:2128	the intestinal barrier	2107:2128	the intestinal barrier of UC mice	2107:2139	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	11	56	theme	signaling	1593:1601	arg1	etc					1636:1638	etc	1636:1638	etc	1636:1638	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	56	theme	signaling	1593:1601	arg1	pathway					1627:1633	MAPK signaling pathway	1612:1633	MAPK signaling pathway	1612:1633	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	56	theme	signaling	1593:1601	arg1	pathway					1603:1609	PI3K-Akt signaling pathway	1584:1609	PI3K-Akt signaling pathway	1584:1609	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	57	theme	interaction	1557:1567	arg1	paths					1569:1573	79 interaction paths	1554:1573	79 interaction paths	1554:1573	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	57	theme	interaction	1557:1567	arg1	pathway					1603:1609	PI3K-Akt signaling pathway	1584:1609	PI3K-Akt signaling pathway	1584:1609	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	15	58	theme	macrophages	2182:2192	arg1	expression					2168:2177	The expression	2164:2177	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination	2164:2263	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	9	59	theme	components	1270:1279	arg1	total					1258:1262	A total	1256:1262	RESULTS A total of 34 components of EHLHP	1248:1288	RESULTS A total of 34 components of EHLHP were identified by UPLC-QE-MS analysis.
36738944	7	60	theme	EHLHP	1068:1072	arg1	pathways					1056:1063	pathways	1056:1063	pathways	1056:1063	The key targets and pathways of EHLHP were selected by the combination of the network pharmacology and transcriptomics results.
36738944	7	60	theme	EHLHP	1068:1072	arg1	targets					1044:1050	The key targets	1036:1050	The key targets	1036:1050	The key targets and pathways of EHLHP were selected by the combination of the network pharmacology and transcriptomics results.
36738944	2	61	from	mechanisms	357:366	arg1	treatment					383:391	the treatment	379:391	the treatment of ulcerative colitis (UC)	379:418	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	16	62	theme	verification	2567:2578	arg1	prediction					2460:2469	the prediction	2456:2469	the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification	2456:2578	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	12	63	dep	regulation	1811:1820	arg1	receptor					1837:1844	B cell receptor	1830:1844	B cell receptor	1830:1844	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	12	63	dep	regulation	1811:1820	arg1	IL-17					1823:1827	IL-17	1823:1827	IL-17	1823:1827	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	10	64	theme	transcriptomics	1385:1399	arg1	analysis					1348:1355	the analysis	1344:1355	the analysis of network pharmacology and transcriptomics	1344:1399	Combined with the analysis of network pharmacology and transcriptomics, there were 262 DEGs between the normal group and the model group, and 151 DEGs between the model group and the EHLHP group.
36738944	3	65	theme	%	536:536	arg1	extraction					546:555	50% ethanol extraction	534:555	50% ethanol extraction of HL	534:561	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	1	66	theme	years	292:296	arg1	hundreds					280:287	hundreds	280:287	hundreds of years in TCM	280:303	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	6	67	theme	transcriptomics	901:915	arg1	method					928:933	the transcriptomics sequencing method	897:933	the transcriptomics sequencing method	897:933	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	2	68	from	HLHP	371:374	arg1	treatment					383:391	the treatment	379:391	the treatment of ulcerative colitis (UC)	379:418	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	5	69	with	network	785:791	arg1	components-targets-pathways					798:824	components-targets-pathways	798:824	components-targets-pathways	798:824	A visualization network with components-targets-pathways on UC treatment were constructed using network pharmacology.
36738944	14	70	theme	Claudin-1	2082:2090	arg1	results					2052:2058	The IHC staining results	2035:2058	The IHC staining results of ZO-1, Occludin and Claudin-1	2035:2090	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	11	71	theme	signaling	1617:1625	arg1	pathway					1627:1633	MAPK signaling pathway	1612:1633	MAPK signaling pathway	1612:1633	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	71	theme	signaling	1617:1625	arg1	pathway					1603:1609	PI3K-Akt signaling pathway	1584:1609	PI3K-Akt signaling pathway	1584:1609	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	10	72	theme	pharmacology	1368:1379	arg1	analysis					1348:1355	the analysis	1344:1355	the analysis of network pharmacology and transcriptomics	1344:1399	Combined with the analysis of network pharmacology and transcriptomics, there were 262 DEGs between the normal group and the model group, and 151 DEGs between the model group and the EHLHP group.
36738944	5	73	theme	network	865:871	arg1	pharmacology					873:884	network pharmacology	865:884	network pharmacology	865:884	A visualization network with components-targets-pathways on UC treatment were constructed using network pharmacology.
36738944	12	74	theme	anti-UC	1674:1680	arg1	mechanisms					1682:1691	the anti-UC mechanisms	1670:1691	the anti-UC mechanisms	1670:1691	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	14	75	theme	Occludin	2069:2076	arg1	results					2052:2058	The IHC staining results	2035:2058	The IHC staining results of ZO-1, Occludin and Claudin-1	2035:2090	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	1	76	from	TCM	301:303	arg1	hundreds					280:287	hundreds	280:287	hundreds of years in TCM	280:303	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	11	77	theme	same	1533:1536	arg1	time					1538:1541	the same time	1529:1541	the same time	1529:1541	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	3	78	theme	HL	560:561	arg1	EHLHP					571:575	EHLHP	571:575	EHLHP	571:575	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	78	theme	HL	560:561	arg1	HP					567:568	HP	567:568	HP (EHLHP)	567:576	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	78	theme	HL	560:561	arg1	extraction					546:555	50% ethanol extraction	534:555	50% ethanol extraction of HL	534:561	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	14	79	theme	ZO-1	2063:2066	arg1	results					2052:2058	The IHC staining results	2035:2058	The IHC staining results of ZO-1, Occludin and Claudin-1	2035:2090	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	1	80	from	years	292:296	arg1	TCM					301:303	TCM	301:303	TCM	301:303	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	13	81	theme	320 mg/kg	1868:1876	arg1	EHLHP					1878:1882	320 mg/kg EHLHP	1868:1882	320 mg/kg EHLHP	1868:1882	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	2	82	theme	effective	319:327	arg1	constituents					329:340	effective constituents	319:340	effective constituents	319:340	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	6	83	theme	expressed	980:988	arg1	DEGs					997:1000	DEGs	997:1000	DEGs	997:1000	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	6	83	theme	expressed	980:988	arg1	genes					990:994	the differentially expressed genes	961:994	the differentially expressed genes (DEGs) of EHLHP in the treatment of UC	961:1033	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	6	84	dep	screen	950:955	arg1	out					957:959	out	957:959	out	957:959	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	1	85	from	hundreds	280:287	arg1	TCM					301:303	TCM	301:303	TCM	301:303	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	13	86	theme	inflammation	2015:2026	arg1	level					2028:2032	inflammation level	2015:2032	inflammation level	2015:2032	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	8	87	theme	EHLHP	1204:1208	arg1	mechanisms					1190:1199	the potential mechanisms	1176:1199	the potential mechanisms of EHLHP on DSS-induced UC mice	1176:1231	Ultimately, the potential mechanisms of EHLHP on DSS-induced UC mice were verified.
36738944	16	88	theme	transcriptomes	2497:2510	arg1	analysis					2485:2492	integrated analysis	2474:2492	integrated analysis of transcriptomes and network pharmacology	2474:2535	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	16	88	theme	transcriptomes	2497:2510	arg1	verification					2567:2578	animal test verification	2555:2578	animal test verification	2555:2578	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	1	89	used	used	236:239	arg2	HLHP					194:197	HLHP	194:197	HLHP	194:197	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	89	used	used	236:239	arg2	Decoction					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction	136:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP)	136:198	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	89	used	used	236:239	arg2	prescription					213:224	a classical prescription	201:224	a classical prescription	201:224	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	14	90	theme	IHC	2039:2041	arg1	results					2052:2058	The IHC staining results	2035:2058	The IHC staining results of ZO-1, Occludin and Claudin-1	2035:2090	The IHC staining results of ZO-1, Occludin and Claudin-1 suggested that the intestinal barrier of UC mice was enhanced by EHLHP.
36738944	3	91	theme	HP	567:568	arg1	verification					663:674	the animal experiment verification	641:674	the animal experiment verification	641:674	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	91	theme	HP	567:568	arg1	transcriptomes					589:602	transcriptomes	589:602	transcriptomes	589:602	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	91	theme	HP	567:568	arg1	pharmacology					616:627	network pharmacology	608:627	network pharmacology	608:627	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	91	theme	HP	567:568	arg1	mechanisms					520:529	the potential anti-UC mechanisms	498:529	the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP)	498:576	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	1	92	theme	ETHNOPHARMACOLOGICAL	136:155	arg1	prescription					213:224	a classical prescription	201:224	a classical prescription	201:224	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	92	theme	ETHNOPHARMACOLOGICAL	136:155	arg1	HLHP					194:197	HLHP	194:197	HLHP	194:197	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	92	theme	ETHNOPHARMACOLOGICAL	136:155	arg1	Decoction					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction	136:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP)	136:198	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	8	93	theme	DSS-induced	1213:1223	arg1	mice					1228:1231	DSS-induced UC mice	1213:1231	DSS-induced UC mice	1213:1231	Ultimately, the potential mechanisms of EHLHP on DSS-induced UC mice were verified.
36738944	16	94	theme	network	2516:2522	arg1	pharmacology					2524:2535	network pharmacology	2516:2535	network pharmacology	2516:2535	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	1	95	theme	Huanglian-Houpo	167:181	arg1	prescription					213:224	a classical prescription	201:224	a classical prescription	201:224	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	95	theme	Huanglian-Houpo	167:181	arg1	HLHP					194:197	HLHP	194:197	HLHP	194:197	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	1	95	theme	Huanglian-Houpo	167:181	arg1	Decoction					183:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction	136:191	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP)	136:198	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	3	96	theme	extraction	546:555	arg1	verification					663:674	the animal experiment verification	641:674	the animal experiment verification	641:674	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	96	theme	extraction	546:555	arg1	transcriptomes					589:602	transcriptomes	589:602	transcriptomes	589:602	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	96	theme	extraction	546:555	arg1	pharmacology					616:627	network pharmacology	608:627	network pharmacology	608:627	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	3	96	theme	extraction	546:555	arg1	mechanisms					520:529	the potential anti-UC mechanisms	498:529	the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP)	498:576	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	16	97	theme	UC	2430:2431	arg1	mice					2433:2436	DSS-induced UC mice	2418:2436	DSS-induced UC mice	2418:2436	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	2	98	dep	constituents	329:340	arg1	the					315:317	the	315:317	the	315:317	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	0	99	theme	mucosal	80:86	arg1	barrier					88:94	intestinal mucosal barrier	69:94	intestinal mucosal barrier	69:94	Huanglian-Houpo extract attenuates DSS-induced UC mice by protecting intestinal mucosal barrier and regulating macrophage polarization.
36738944	13	100	theme	value	2005:2009	arg1	decrease					1989:1996	the decrease	1985:1996	the decrease of DAI value and inflammation level	1985:2032	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	15	101	theme	polarization	2340:2351	arg1	M1					2353:2354	macrophage polarization M1	2329:2354	macrophage polarization M1 to M2	2329:2360	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	5	102	from	network	785:791	arg1	treatment					832:840	UC treatment	829:840	UC treatment	829:840	A visualization network with components-targets-pathways on UC treatment were constructed using network pharmacology.
36738944	6	103	theme	EHLHP	1006:1010	arg1	DEGs					997:1000	DEGs	997:1000	DEGs	997:1000	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	6	103	theme	EHLHP	1006:1010	arg1	genes					990:994	the differentially expressed genes	961:994	the differentially expressed genes (DEGs) of EHLHP in the treatment of UC	961:1033	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	10	104	theme	normal	1434:1439	arg1	group					1441:1445	the normal group	1430:1445	the normal group	1430:1445	Combined with the analysis of network pharmacology and transcriptomics, there were 262 DEGs between the normal group and the model group, and 151 DEGs between the model group and the EHLHP group.
36738944	0	105	theme	macrophage	111:120	arg1	polarization					122:133	macrophage polarization	111:133	macrophage polarization	111:133	Huanglian-Houpo extract attenuates DSS-induced UC mice by protecting intestinal mucosal barrier and regulating macrophage polarization.
36738944	9	106	theme	EHLHP	1284:1288	arg1	components					1270:1279	34 components	1267:1279	34 components of EHLHP	1267:1288	RESULTS A total of 34 components of EHLHP were identified by UPLC-QE-MS analysis.
36738944	4	107	dep	METHODS	677:683	arg1	identified					699:708	identified	699:708	identified the chemical composition of EHLHP via UPLC-QE-MS analysis	699:766	METHODS Primarily, we identified the chemical composition of EHLHP via UPLC-QE-MS analysis.
36738944	2	108	from	constituents	329:340	arg1	treatment					383:391	the treatment	379:391	the treatment of ulcerative colitis (UC)	379:418	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	6	109	from	treatment	1019:1027	arg1	DEGs					997:1000	DEGs	997:1000	DEGs	997:1000	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	6	109	from	treatment	1019:1027	arg1	genes					990:994	the differentially expressed genes	961:994	the differentially expressed genes (DEGs) of EHLHP in the treatment of UC	961:1033	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	4	110	theme	EHLHP	738:742	arg1	composition					723:733	the chemical composition	710:733	the chemical composition of EHLHP	710:742	METHODS Primarily, we identified the chemical composition of EHLHP via UPLC-QE-MS analysis.
36738944	12	111	theme	immune	1804:1809	arg1	regulation					1811:1820	immune regulation	1804:1820	immune regulation (IL-17, B cell receptor)	1804:1845	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	7	112	dep	pharmacology	1122:1133	arg1	results					1155:1161	results	1155:1161	results	1155:1161	The key targets and pathways of EHLHP were selected by the combination of the network pharmacology and transcriptomics results.
36738944	4	113	theme	UPLC-QE-MS	748:757	arg1	analysis					759:766	UPLC-QE-MS analysis	748:766	UPLC-QE-MS analysis	748:766	METHODS Primarily, we identified the chemical composition of EHLHP via UPLC-QE-MS analysis.
36738944	9	114	theme	UPLC-QE-MS	1309:1318	arg1	analysis					1320:1327	UPLC-QE-MS analysis	1309:1327	UPLC-QE-MS analysis	1309:1327	RESULTS A total of 34 components of EHLHP were identified by UPLC-QE-MS analysis.
36738944	12	115	theme	B	1830:1830	arg1	receptor					1837:1844	B cell receptor	1830:1844	B cell receptor	1830:1844	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	12	115	theme	B	1830:1830	arg1	IL-17					1823:1827	IL-17	1823:1827	IL-17	1823:1827	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	2	116	theme	ulcerative	396:405	arg1	UC					416:417	UC	416:417	UC	416:417	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	2	116	theme	ulcerative	396:405	arg1	colitis					407:413	ulcerative colitis	396:413	ulcerative colitis (UC)	396:418	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	0	117	theme	DSS-induced	35:45	arg1	mice					50:53	DSS-induced UC mice	35:53	DSS-induced UC mice	35:53	Huanglian-Houpo extract attenuates DSS-induced UC mice by protecting intestinal mucosal barrier and regulating macrophage polarization.
36738944	17	118	theme	TCM	2647:2649	arg1	formula					2661:2667	TCM classical formula	2647:2667	TCM classical formula	2647:2667	It would provide a viable strategy to elucidate the mechanisms of TCM classical formula.
36738944	12	119	dep	pathway	1764:1770	arg1	cGMP-PKG					1790:1797	cGMP-PKG	1790:1797	cGMP-PKG	1790:1797	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	12	119	dep	pathway	1764:1770	arg1	TNF-α					1783:1787	TNF-α	1783:1787	TNF-α	1783:1787	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	12	119	dep	pathway	1764:1770	arg1	JAK-STAT					1773:1780	JAK-STAT	1773:1780	JAK-STAT	1773:1780	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	17	120	theme	formula	2661:2667	arg1	mechanisms					2633:2642	the mechanisms	2629:2642	the mechanisms of TCM classical formula	2629:2667	It would provide a viable strategy to elucidate the mechanisms of TCM classical formula.
36738944	6	121	from	genes	990:994	arg1	treatment					1019:1027	the treatment	1015:1027	the treatment of UC	1015:1033	And then, the transcriptomics sequencing method was applied to screen out the differentially expressed genes (DEGs) of EHLHP in the treatment of UC.
36738944	3	122	theme	network	608:614	arg1	pharmacology					616:627	network pharmacology	608:627	network pharmacology	608:627	AIM OF THE STUDY This study aimed to reveal the potential anti-UC mechanisms of 50% ethanol extraction of HL and HP (EHLHP), combining transcriptomes and network pharmacology, as well as the animal experiment verification.
36738944	7	123	theme	network	1114:1120	arg1	pharmacology					1122:1133	the network pharmacology	1110:1133	the network pharmacology	1110:1133	The key targets and pathways of EHLHP were selected by the combination of the network pharmacology and transcriptomics results.
36738944	15	124	dep	F4/80+	2214:2219	arg1	NK1.1+					2237:2242	NK1.1+	2237:2242	NK1.1+	2237:2242	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	15	124	dep	F4/80+	2214:2219	arg1	Gr-1+					2230:2234	Gr-1+	2230:2234	Gr-1+	2230:2234	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	15	124	dep	F4/80+	2214:2219	arg1	CD11c+					2222:2227	CD11c+	2222:2227	CD11c+	2222:2227	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
36738944	12	125	theme	signaling	1754:1762	arg1	pathway					1764:1770	inflammatory signaling pathway	1741:1770	inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG)	1741:1798	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	10	126	theme	model	1493:1497	arg1	group					1499:1503	the model group	1489:1503	the model group	1489:1503	Combined with the analysis of network pharmacology and transcriptomics, there were 262 DEGs between the normal group and the model group, and 151 DEGs between the model group and the EHLHP group.
36738944	10	127	theme	model	1455:1459	arg1	group					1461:1465	the model group	1451:1465	the model group	1451:1465	Combined with the analysis of network pharmacology and transcriptomics, there were 262 DEGs between the normal group and the model group, and 151 DEGs between the model group and the EHLHP group.
36738944	13	128	theme	typical	1925:1931	arg1	symptoms					1933:1940	these typical symptoms	1919:1940	these typical symptoms	1919:1940	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	10	129	theme	network	1360:1366	arg1	pharmacology					1368:1379	network pharmacology	1360:1379	network pharmacology	1360:1379	Combined with the analysis of network pharmacology and transcriptomics, there were 262 DEGs between the normal group and the model group, and 151 DEGs between the model group and the EHLHP group.
36738944	2	130	theme	underlying	346:355	arg1	mechanisms					357:366	underlying mechanisms	346:366	underlying mechanisms	346:366	However, the effective constituents and underlying mechanisms of HLHP in the treatment of ulcerative colitis (UC) have not been fully investigated.
36738944	1	131	theme	gastrointestinal	250:265	arg1	diseases					267:274	gastrointestinal diseases	250:274	gastrointestinal diseases	250:274	ETHNOPHARMACOLOGICAL RELEVANCE Huanglian-Houpo Decoction (HLHP), a classical prescription, has been used to treat gastrointestinal diseases for hundreds of years in TCM.
36738944	16	132	theme	extract	2407:2413	arg1	mechanisms					2388:2397	The potential mechanisms	2374:2397	The potential mechanisms of HLHP extract on DSS-induced UC mice	2374:2436	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	12	133	theme	signaling	1722:1730	arg1	pathway					1732:1738	calcium signaling pathway	1714:1738	calcium signaling pathway	1714:1738	These results indicated that the anti-UC mechanisms would be involved in calcium signaling pathway, inflammatory signaling pathway (JAK-STAT, TNF-α, cGMP-PKG) and immune regulation (IL-17, B cell receptor).
36738944	16	134	theme	test	2562:2565	arg1	verification					2567:2578	animal test verification	2555:2578	animal test verification	2555:2578	CONCLUSION The potential mechanisms of HLHP extract on DSS-induced UC mice were revealed, by the prediction of integrated analysis of transcriptomes and network pharmacology, and subsequently animal test verification.
36738944	10	135	theme	EHLHP	1513:1517	arg1	group					1519:1523	the EHLHP group	1509:1523	the EHLHP group	1509:1523	Combined with the analysis of network pharmacology and transcriptomics, there were 262 DEGs between the normal group and the model group, and 151 DEGs between the model group and the EHLHP group.
36738944	11	136	theme	PI3K-Akt	1584:1591	arg1	etc					1636:1638	etc	1636:1638	etc	1636:1638	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	136	theme	PI3K-Akt	1584:1591	arg1	pathway					1627:1633	MAPK signaling pathway	1612:1633	MAPK signaling pathway	1612:1633	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	11	136	theme	PI3K-Akt	1584:1591	arg1	pathway					1603:1609	PI3K-Akt signaling pathway	1584:1609	PI3K-Akt signaling pathway	1584:1609	At the same time, there are 79 interaction paths, such as PI3K-Akt signaling pathway, MAPK signaling pathway, etc.
36738944	13	137	theme	UC	1910:1911	arg1	mice					1913:1916	DSS induced UC mice	1898:1916	DSS induced UC mice	1898:1916	After 160 mg/kg and 320 mg/kg EHLHP were given to DSS induced UC mice, these typical symptoms could be significantly alleviated, such as the decrease of DAI value and inflammation level.
36738944	15	138	theme	cells	2205:2209	arg1	expression					2168:2177	The expression	2164:2177	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination	2164:2263	The expression of macrophages and immune cells in F4/80+, CD11c+, Gr-1+, NK1.1+ by FCM determination indicated that EHLHP could suppress UC by immunosuppression and macrophage polarization M1 to M2.
35333990	8	0	theme	interleukin	989:999	arg1	IL					1002:1003	interleukin (IL)-1β	989:1007	interleukin (IL)-1β	989:1007	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	8	1	theme	quantitative	1058:1069	arg1	PCR					1071:1073	quantitative PCR	1058:1073	quantitative PCR	1058:1073	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	7	2	theme	intestinal	875:884	arg1	tract					886:890	the intestinal tract	871:890	the intestinal tract	871:890	After euthanizing the mice, pathological sections of the intestinal tract were prepared and stained with hematoxylin and eosin (H&E).
35333990	4	3	theme	lipopolysaccharide	566:583	arg1	effect					534:539	the effect	530:539	the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model	530:647	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	4	4	theme	present	499:505	arg1	study					507:511	the present study	495:511	the present study	495:511	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	6	5	theme	large	800:804	arg1	intestines					806:815	the small and large intestines	786:815	the small and large intestines	786:815	Three months later, DNA was extracted from stool, and RNA from the small and large intestines.
35333990	4	6	theme	gingivalis-derived	547:564	arg1	Pg-LPS					586:591	Pg-LPS	586:591	Pg-LPS	586:591	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	4	6	theme	gingivalis-derived	547:564	arg1	lipopolysaccharide					566:583	gingivalis-derived lipopolysaccharide	547:583	P. gingivalis-derived lipopolysaccharide (Pg-LPS)	544:592	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	8	7	theme	factor	967:972	arg1	TNF-α					981:985	TNF-α	981:985	TNF-α	981:985	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	8	7	theme	factor	967:972	arg1	alpha					974:978	Tumor necrosis factor alpha	952:978	Tumor necrosis factor alpha (TNF-α)	952:986	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	10	8	theme	Quantitative	1198:1209	arg1	Insights					1211:1218	Quantitative Insights	1198:1218	Quantitative Insights into Microbial Ecology 2	1198:1243	Microbial diversity and composition were analyzed using Quantitative Insights into Microbial Ecology 2.
35333990	10	9	theme	Microbial	1142:1150	arg1	diversity					1152:1160	Microbial diversity	1142:1160	Microbial diversity	1142:1160	Microbial diversity and composition were analyzed using Quantitative Insights into Microbial Ecology 2.
35333990	3	10	theme	P.	365:366	arg1	gingivalis					368:377	P. gingivalis	365:377	P. gingivalis	365:377	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	16	11	theme	periodontal	1733:1743	arg1	pathogens					1745:1753	periodontal pathogens	1733:1753	periodontal pathogens	1733:1753	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	5	12	theme	Eight-week-old	650:663	arg1	mice					674:677	Eight-week-old C57BL/6J mice	650:677	Eight-week-old C57BL/6J mice	650:677	Eight-week-old C57BL/6J mice were intraperitoneally administered Pg-LPS.
35333990	6	13	theme	small	790:794	arg1	intestines					806:815	the small and large intestines	786:815	the small and large intestines	786:815	Three months later, DNA was extracted from stool, and RNA from the small and large intestines.
35333990	3	14	theme	Oral	342:345	arg1	administration					347:360	Oral administration	342:360	Oral administration of P. gingivalis	342:377	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	13	15	from	differences	1398:1408	arg1	levels					1422:1427	the mRNA levels	1413:1427	the mRNA levels of IL-1β, IL-6, and TNF-α	1413:1453	No significant differences in the mRNA levels of IL-1β, IL-6, and TNF-α were observed between the groups.
35333990	8	16	theme	necrosis	958:965	arg1	TNF-α					981:985	TNF-α	981:985	TNF-α	981:985	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	8	16	theme	necrosis	958:965	arg1	alpha					974:978	Tumor necrosis factor alpha	952:978	Tumor necrosis factor alpha (TNF-α)	952:986	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	2	17	theme	tissue	282:287	arg1	breakdown					289:297	periodontal tissue breakdown	270:297	periodontal tissue breakdown	270:297	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	7	18	theme	pathological	846:857	arg1	sections					859:866	pathological sections	846:866	pathological sections of the intestinal tract	846:890	After euthanizing the mice, pathological sections of the intestinal tract were prepared and stained with hematoxylin and eosin (H&E).
35333990	3	19	theme	systemic	423:430	arg1	administration					432:445	no systemic administration	420:445	no systemic administration of P. gingivalis	420:462	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	12	20	located	observed	1362:1369	arg1	H&E					1378:1380	the H&E	1374:1380	the H&E	1374:1380	No significant inflammatory changes were observed in the H&E.
35333990	12	20	located	observed	1362:1369	arg2	changes					1349:1355	No significant inflammatory changes	1321:1355	No significant inflammatory changes	1321:1355	No significant inflammatory changes were observed in the H&E.
35333990	11	21	theme	microbial	1274:1282	arg1	function					1284:1291	microbial function	1274:1291	microbial function	1274:1291	Furthermore, alterations in microbial function were performed by PICRUSt2.
35333990	1	22	theme	Gut	87:89	arg1	dysbiosis					91:99	Gut dysbiosis	87:99	Gut dysbiosis	87:99	Gut dysbiosis induces 'leaky gut,' a condition associated with diabetes, NASH, and various auto-immune diseases.
35333990	15	23	theme	metagenomic	1577:1587	arg1	analysis					1589:1596	A predictive metagenomic analysis	1564:1596	A predictive metagenomic analysis by PICRUSt2 and STAMP	1564:1618	A predictive metagenomic analysis by PICRUSt2 and STAMP showed that 47 pathways increased and 17 pathways decreased after Pg-LPS administration.
35333990	4	24	theme	mouse	637:641	arg1	model					643:647	our established mouse model	621:647	our established mouse model	621:647	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	0	25	theme	flora	4:8	arg1	alterations					10:20	Gut flora alterations	0:20	Gut flora alterations due to lipopolysaccharide	0:46	Gut flora alterations due to lipopolysaccharide derived from Porphyromonas gingivalis.
35333990	13	26	theme	significant	1386:1396	arg1	differences					1398:1408	No significant differences	1383:1408	No significant differences in the mRNA levels of IL-1β, IL-6, and TNF-α	1383:1453	No significant differences in the mRNA levels of IL-1β, IL-6, and TNF-α were observed between the groups.
35333990	14	27	theme	Allobacterium	1538:1550	arg1	abundance					1525:1533	the abundance	1521:1533	the abundance of Allobacterium in the gut	1521:1561	Pg-LPS administration decreased the abundance of Allobacterium in the gut.
35333990	0	28	theme	Gut	0:2	arg1	alterations					10:20	Gut flora alterations	0:20	Gut flora alterations due to lipopolysaccharide	0:46	Gut flora alterations due to lipopolysaccharide derived from Porphyromonas gingivalis.
35333990	12	29	theme	inflammatory	1336:1347	arg1	changes					1349:1355	No significant inflammatory changes	1321:1355	No significant inflammatory changes	1321:1355	No significant inflammatory changes were observed in the H&E.
35333990	16	30	from	changes	1765:1771	arg1	flora					1791:1795	the intestinal flora	1776:1795	the intestinal flora which may affect the physiological functions of the intestinal tract	1776:1864	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	3	31	theme	gingivalis	368:377	arg1	administration					347:360	Oral administration	342:360	Oral administration of P. gingivalis	342:377	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	11	32	from	alterations	1259:1269	arg1	function					1284:1291	microbial function	1274:1291	microbial function	1274:1291	Furthermore, alterations in microbial function were performed by PICRUSt2.
35333990	8	33	theme	expression	1019:1028	arg1	levels					1030:1035	IL-6 expression levels	1014:1035	IL-6 expression levels	1014:1035	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	14	34	theme	Pg-LPS	1489:1494	arg1	administration					1496:1509	Pg-LPS administration	1489:1509	Pg-LPS administration	1489:1509	Pg-LPS administration decreased the abundance of Allobacterium in the gut.
35333990	8	35	theme	Tumor	952:956	arg1	TNF-α					981:985	TNF-α	981:985	TNF-α	981:985	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	8	35	theme	Tumor	952:956	arg1	alpha					974:978	Tumor necrosis factor alpha	952:978	Tumor necrosis factor alpha (TNF-α)	952:986	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	3	36	theme	gingivalis	453:462	arg1	administration					432:445	no systemic administration	420:445	no systemic administration of P. gingivalis	420:462	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	9	37	theme	amplicon	1094:1101	arg1	analysis					1103:1110	16S rRNA gene PCR amplicon analysis	1076:1110	16S rRNA gene PCR amplicon analysis data	1076:1115	16S rRNA gene PCR amplicon analysis data were acquired using NGS.
35333990	8	38	theme	IL-6	1014:1017	arg1	levels					1030:1035	IL-6 expression levels	1014:1035	IL-6 expression levels	1014:1035	Tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, and IL-6 expression levels were evaluated using quantitative PCR.
35333990	1	39	theme	leaky	110:114	arg1	gut					116:118	'leaky gut	109:118	'leaky gut	109:118	Gut dysbiosis induces 'leaky gut,' a condition associated with diabetes, NASH, and various auto-immune diseases.
35333990	1	39	theme	leaky	110:114	arg1	condition					124:132	a condition	122:132	' a condition associated with diabetes, NASH, and various auto-immune diseases	120:197	Gut dysbiosis induces 'leaky gut,' a condition associated with diabetes, NASH, and various auto-immune diseases.
35333990	15	40	theme	Pg-LPS	1686:1691	arg1	administration					1693:1706	Pg-LPS administration	1686:1706	Pg-LPS administration	1686:1706	A predictive metagenomic analysis by PICRUSt2 and STAMP showed that 47 pathways increased and 17 pathways decreased after Pg-LPS administration.
35333990	1	41	theme	auto-immune	178:188	arg1	diseases					190:197	various auto-immune diseases	170:197	various auto-immune diseases	170:197	Gut dysbiosis induces 'leaky gut,' a condition associated with diabetes, NASH, and various auto-immune diseases.
35333990	13	42	theme	IL-6	1439:1442	arg1	levels					1422:1427	the mRNA levels	1413:1427	the mRNA levels of IL-1β, IL-6, and TNF-α	1413:1453	No significant differences in the mRNA levels of IL-1β, IL-6, and TNF-α were observed between the groups.
35333990	16	43	theme	tract	1860:1864	arg1	functions					1832:1840	the physiological functions	1814:1840	the physiological functions of the intestinal tract	1814:1864	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	4	44	theme	established	625:635	arg1	model					643:647	our established mouse model	621:647	our established mouse model	621:647	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	4	45	theme	model	643:647	arg1	flora					612:616	the intestinal flora	597:616	the intestinal flora of our established mouse model	597:647	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	16	46	theme	Systemic	1709:1716	arg1	application					1718:1728	Systemic application	1709:1728	Systemic application of periodontal pathogens	1709:1753	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	4	47	dep	lipopolysaccharide	566:583	arg1	P.					544:545	P. gingivalis-derived lipopolysaccharide (Pg-LPS)	544:592	P. gingivalis-derived lipopolysaccharide (Pg-LPS)	544:592	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	4	48	from	effect	534:539	arg1	flora					612:616	the intestinal flora	597:616	the intestinal flora of our established mouse model	597:647	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	3	49	theme	P.	450:451	arg1	gingivalis					453:462	P. gingivalis	450:462	P. gingivalis	450:462	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	5	50	theme	C57BL/6J	665:672	arg1	mice					674:677	Eight-week-old C57BL/6J mice	650:677	Eight-week-old C57BL/6J mice	650:677	Eight-week-old C57BL/6J mice were intraperitoneally administered Pg-LPS.
35333990	16	51	theme	pathogens	1745:1753	arg1	application					1718:1728	Systemic application	1709:1728	Systemic application of periodontal pathogens	1709:1753	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	12	52	theme	significant	1324:1334	arg1	changes					1349:1355	No significant inflammatory changes	1321:1355	No significant inflammatory changes	1321:1355	No significant inflammatory changes were observed in the H&E.
35333990	9	53	theme	16S	1076:1078	arg1	analysis					1103:1110	16S rRNA gene PCR amplicon analysis	1076:1110	16S rRNA gene PCR amplicon analysis data	1076:1115	16S rRNA gene PCR amplicon analysis data were acquired using NGS.
35333990	3	54	theme	mice	404:407	arg1	model					409:413	mice model	404:413	mice model	404:413	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	13	55	theme	IL-1β	1432:1436	arg1	levels					1422:1427	the mRNA levels	1413:1427	the mRNA levels of IL-1β, IL-6, and TNF-α	1413:1453	No significant differences in the mRNA levels of IL-1β, IL-6, and TNF-α were observed between the groups.
35333990	16	56	theme	physiological	1818:1830	arg1	functions					1832:1840	the physiological functions	1814:1840	the physiological functions of the intestinal tract	1814:1864	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	2	57	theme	Porphyromonas	200:212	arg1	bacterium					247:255	a periodontopathic bacterium	228:255	a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow	228:339	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	2	57	theme	Porphyromonas	200:212	arg1	gingivalis					214:223	Porphyromonas gingivalis	200:223	Porphyromonas gingivalis	200:223	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	9	58	theme	rRNA	1080:1083	arg1	analysis					1103:1110	16S rRNA gene PCR amplicon analysis	1076:1110	16S rRNA gene PCR amplicon analysis data	1076:1115	16S rRNA gene PCR amplicon analysis data were acquired using NGS.
35333990	13	59	theme	mRNA	1417:1420	arg1	levels					1422:1427	the mRNA levels	1413:1427	the mRNA levels of IL-1β, IL-6, and TNF-α	1413:1453	No significant differences in the mRNA levels of IL-1β, IL-6, and TNF-α were observed between the groups.
35333990	16	60	theme	intestinal	1780:1789	arg1	flora					1791:1795	the intestinal flora	1776:1795	the intestinal flora which may affect the physiological functions of the intestinal tract	1776:1864	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	16	61	theme	intestinal	1849:1858	arg1	tract					1860:1864	the intestinal tract	1845:1864	the intestinal tract	1845:1864	Systemic application of periodontal pathogens may cause changes in the intestinal flora which may affect the physiological functions of the intestinal tract.
35333990	9	62	theme	gene	1085:1088	arg1	analysis					1103:1110	16S rRNA gene PCR amplicon analysis	1076:1110	16S rRNA gene PCR amplicon analysis data	1076:1115	16S rRNA gene PCR amplicon analysis data were acquired using NGS.
35333990	2	63	theme	blood	330:334	arg1	flow					336:339	the systemic blood flow	317:339	the systemic blood flow	317:339	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	15	64	theme	predictive	1566:1575	arg1	analysis					1589:1596	A predictive metagenomic analysis	1564:1596	A predictive metagenomic analysis by PICRUSt2 and STAMP	1564:1618	A predictive metagenomic analysis by PICRUSt2 and STAMP showed that 47 pathways increased and 17 pathways decreased after Pg-LPS administration.
35333990	2	65	theme	periodontal	270:280	arg1	breakdown					289:297	periodontal tissue breakdown	270:297	periodontal tissue breakdown	270:297	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	14	66	from	abundance	1525:1533	arg1	gut					1559:1561	the gut	1555:1561	the gut	1555:1561	Pg-LPS administration decreased the abundance of Allobacterium in the gut.
35333990	9	67	theme	PCR	1090:1092	arg1	analysis					1103:1110	16S rRNA gene PCR amplicon analysis	1076:1110	16S rRNA gene PCR amplicon analysis data	1076:1115	16S rRNA gene PCR amplicon analysis data were acquired using NGS.
35333990	2	68	theme	systemic	321:328	arg1	flow					336:339	the systemic blood flow	317:339	the systemic blood flow	317:339	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	7	69	theme	tract	886:890	arg1	sections					859:866	pathological sections	846:866	pathological sections of the intestinal tract	846:890	After euthanizing the mice, pathological sections of the intestinal tract were prepared and stained with hematoxylin and eosin (H&E).
35333990	4	70	link	gingivalis-derived	547:564	arg1	Pg-LPS					586:591	Pg-LPS	586:591	Pg-LPS	586:591	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	4	70	link	gingivalis-derived	547:564	arg1	lipopolysaccharide					566:583	gingivalis-derived lipopolysaccharide	547:583	P. gingivalis-derived lipopolysaccharide (Pg-LPS)	544:592	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	1	71	theme	various	170:176	arg1	diseases					190:197	various auto-immune diseases	170:197	various auto-immune diseases	170:197	Gut dysbiosis induces 'leaky gut,' a condition associated with diabetes, NASH, and various auto-immune diseases.
35333990	13	72	theme	TNF-α	1449:1453	arg1	levels					1422:1427	the mRNA levels	1413:1427	the mRNA levels of IL-1β, IL-6, and TNF-α	1413:1453	No significant differences in the mRNA levels of IL-1β, IL-6, and TNF-α were observed between the groups.
35333990	4	73	theme	intestinal	601:610	arg1	flora					612:616	the intestinal flora	597:616	the intestinal flora of our established mouse model	597:647	In the present study, we investigated the effect of P. gingivalis-derived lipopolysaccharide (Pg-LPS) on the intestinal flora of our established mouse model.
35333990	0	74	theme	Porphyromonas	61:73	arg1	gingivalis					75:84	Porphyromonas gingivalis	61:84	Porphyromonas gingivalis	61:84	Gut flora alterations due to lipopolysaccharide derived from Porphyromonas gingivalis.
35333990	2	75	theme	periodontopathic	230:245	arg1	bacterium					247:255	a periodontopathic bacterium	228:255	a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow	228:339	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	2	75	theme	periodontopathic	230:245	arg1	gingivalis					214:223	Porphyromonas gingivalis	200:223	Porphyromonas gingivalis	200:223	Porphyromonas gingivalis is a periodontopathic bacterium which causes periodontal tissue breakdown, and often enters the systemic blood flow.
35333990	9	76	theme	analysis	1103:1110	arg1	data					1112:1115	16S rRNA gene PCR amplicon analysis data	1076:1115	16S rRNA gene PCR amplicon analysis data	1076:1115	16S rRNA gene PCR amplicon analysis data were acquired using NGS.
35333990	3	77	theme	gut	387:389	arg1	dysbiosis					391:399	gut dysbiosis	387:399	gut dysbiosis	387:399	Oral administration of P. gingivalis induced gut dysbiosis in mice model, but no systemic administration of P. gingivalis has been reported thus far.
35333990	10	78	theme	Microbial	1225:1233	arg1	Ecology					1235:1241	Microbial Ecology 2	1225:1243	Microbial Ecology 2	1225:1243	Microbial diversity and composition were analyzed using Quantitative Insights into Microbial Ecology 2.
35939489	7	0	theme	inflammation	1143:1154	arg1	biomarkers					1129:1138	biomarkers	1129:1138	biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes	1129:1205	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	4	1	theme	Low-grade	805:813	arg1	inflammation					815:826	Low-grade inflammation	805:826	Low-grade inflammation	805:826	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	2	2	theme	fiber	297:301	arg1	fractions					303:311	protein and fiber fractions	285:311	fractions	303:311	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	1	3	theme	colon	215:219	arg1	health					221:226	colon health	215:226	colon health	215:226	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
35939489	9	4	theme	fecal	1316:1320	arg1	lipocalin-2					1322:1332	fecal lipocalin-2	1316:1332	fecal lipocalin-2	1316:1332	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	10	5	contain	had	1539:1541	arg1	mice					1529:1532	the mice	1525:1532	the mice that had faba-bean proteins	1525:1560	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	10	5	contain	had	1539:1541	arg2	proteins					1553:1560	faba-bean proteins	1543:1560	faba-bean proteins	1543:1560	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	10	6	theme	Proteobacteria	1584:1597	arg1	amount					1574:1579	a lower amount	1566:1579	a lower amount of Proteobacteria	1566:1597	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	10	6	theme	Proteobacteria	1584:1597	arg1	Proteobacteria					1584:1597	Proteobacteria	1584:1597	Proteobacteria	1584:1597	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	3	7	theme	male	483:486	arg1	mice					488:491	sixty C57BL/6JRj male mice	466:491	sixty C57BL/6JRj male mice	466:491	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	8	theme	=	595:595	arg1	n					593:593	n = 12	593:598	n = 12	593:598	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	8	theme	=	595:595	arg1	groups					585:590	four groups	580:590	four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24)	580:802	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	9	theme	sixty	466:470	arg1	mice					488:491	sixty C57BL/6JRj male mice	466:491	sixty C57BL/6JRj male mice	466:491	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	10	dep	WD	515:516	arg1	fat					523:525	41% fat	519:525	41% fat	519:525	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	10	dep	WD	515:516	arg1	carbohydrates					532:544	43% carbohydrates	528:544	43% carbohydrates	528:544	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	9	11	theme	disease	1346:1352	arg1	score					1369:1373	disease activity index score	1346:1373	disease activity index score	1346:1373	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	12	12	theme	inflammation	1777:1788	arg1	model					1790:1794	a low-grade inflammation model	1765:1794	a low-grade inflammation model	1765:1794	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	9	13	theme	index	1363:1367	arg1	score					1369:1373	disease activity index score	1346:1373	disease activity index score	1346:1373	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	4	14	theme	sodium	854:859	arg1	DSS					870:872	DSS	870:872	DSS	870:872	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	4	14	theme	sodium	854:859	arg1	sulfate					861:867	1% dextran sodium sulfate	843:867	1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial	843:922	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	5	15	from	Half	925:928	arg1	group					942:946	group 4)	942:949	group 4)	942:949	Half (n = 12) in group 4) were given only water (controls).
35939489	3	16	theme	43	528:529	arg1	%					530:530	%	530:530	%	530:530	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	9	17	theme	bean	1401:1404	arg1	fractions					1406:1414	faba bean fractions	1396:1414	faba bean fractions	1396:1414	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	11	18	contain	had	1693:1695	arg2	fraction					1703:1710	fiber fraction	1697:1710	fiber fraction from faba-beans	1697:1726	The Actinobacteria abundance was also lower in the group that had fiber fraction from faba-beans.
35939489	11	18	contain	had	1693:1695	arg1	group					1682:1686	the group	1678:1686	the group that had fiber fraction from faba-beans	1678:1726	The Actinobacteria abundance was also lower in the group that had fiber fraction from faba-beans.
35939489	9	19	from	presence	1384:1391	arg1	WD					1419:1420	WD	1419:1420	WD	1419:1420	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	4	20	theme	last	897:900	arg1	days					906:909	the last six days	893:909	the last six days of the trial	893:922	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	12	21	with	WD	1838:1839	arg1	fractions					1856:1864	faba bean fractions	1846:1864	faba bean fractions	1846:1864	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	6	22	dep	weight	1004:1009	arg1	assays					1057:1062	tolerance assays	1047:1062	tolerance assays	1047:1062	Prior to DSS, body weight, energy intake, glucose and insulin tolerance assays were performed.
35939489	7	23	from	status	1093:1098	arg1	colon					1107:1111	the colon	1103:1111	the colon	1103:1111	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	12	24	theme	colonic	1918:1924	arg1	microbiota					1926:1935	colonic microbiota	1918:1935	colonic microbiota	1918:1935	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	5	25	dep	given	956:960	arg1	controls					974:981	controls	974:981	controls	974:981	Half (n = 12) in group 4) were given only water (controls).
35939489	12	26	theme	bean	1851:1854	arg1	fractions					1856:1864	faba bean fractions	1846:1864	faba bean fractions	1846:1864	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	3	27	theme	faba-bean	662:670	arg1	proteins					672:679	faba-bean proteins	662:679	faba-bean proteins	662:679	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	6	28	theme	energy	1012:1017	arg1	intake					1019:1024	energy intake	1012:1024	energy intake	1012:1024	Prior to DSS, body weight, energy intake, glucose and insulin tolerance assays were performed.
35939489	10	29	from	group	1612:1616	arg1	WD					1627:1628	plain WD	1621:1628	plain WD	1621:1628	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	3	30	theme	faba-bean	723:731	arg1	fibers					733:738	faba-bean fibers	723:738	faba-bean fibers	723:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	12	31	theme	marginal	1870:1877	arg1	effects					1879:1885	marginal effects	1870:1885	marginal effects	1870:1885	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	0	32	theme	DSS	81:83	arg1	dose					73:76	low dose	69:76	low dose of DSS	69:83	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	2	33	theme	mice-model	401:410	arg1	inflammation					388:399	a low-grade inflammation mice-model	376:410	a low-grade inflammation mice-model when incorporated in a Western diet (WD)	376:451	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	0	34	theme	Intestinal	0:9	arg1	effect					11:16	Intestinal effect	0:16	Intestinal effect of faba bean fractions in WD-fed mice	0:54	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	2	35	theme	low-grade	378:386	arg1	inflammation					388:399	a low-grade inflammation mice-model	376:410	a low-grade inflammation mice-model when incorporated in a Western diet (WD)	376:451	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	8	36	theme	Fecal	1208:1212	arg1	composition					1225:1235	Fecal microbiota composition	1208:1235	Fecal microbiota composition	1208:1235	Fecal microbiota composition was assessed by 16S rRNA gene sequencing.
35939489	7	37	theme	genes	1201:1205	arg1	analyses					1168:1175	qRT-PCR analyses	1160:1175	qRT-PCR analyses	1160:1175	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	7	37	theme	genes	1201:1205	arg1	inflammation					1143:1154	inflammation	1143:1154	inflammation	1143:1154	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	2	38	theme	microbiota	350:359	arg1	composition					361:371	microbiota composition	350:371	microbiota composition	350:371	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	0	39	theme	bean	26:29	arg1	fractions					31:39	faba bean fractions	21:39	faba bean fractions	21:39	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	7	40	theme	inflammatory	1180:1191	arg1	genes					1201:1205	inflammatory related genes	1180:1205	inflammatory related genes	1180:1205	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	9	41	theme	inflammatory	1485:1496	arg1	genes					1509:1513	inflammatory associated genes	1485:1513	inflammatory associated genes	1485:1513	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	12	42	theme	fiber	1827:1831	arg1	replacement					1797:1807	replacement	1797:1807	replacement of protein and or fiber in a WD with faba bean fractions	1797:1864	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	6	43	theme	tolerance	1047:1055	arg1	assays					1057:1062	tolerance assays	1047:1062	tolerance assays	1047:1062	Prior to DSS, body weight, energy intake, glucose and insulin tolerance assays were performed.
35939489	7	44	theme	analyses	1168:1175	arg1	biomarkers					1129:1138	biomarkers	1129:1138	biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes	1129:1205	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	9	45	theme	1	1279:1279	arg1	%					1280:1280	%	1280:1280	%	1280:1280	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	4	46	theme	1	843:843	arg1	%					844:844	%	844:844	%	844:844	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	3	47	dep	WD	792:793	arg1	4					783:783	4	783:783	4	783:783	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	5	48	theme	=	933:933	arg1	n					931:931	n = 12	931:936	n = 12	931:936	Half (n = 12) in group 4) were given only water (controls).
35939489	5	48	theme	=	933:933	arg1	Half					925:928	Half	925:928	Half (n = 12) in group 4)	925:949	Half (n = 12) in group 4) were given only water (controls).
35939489	9	49	theme	DSS	1282:1284	arg1	treatment					1286:1294	1% DSS treatment	1279:1294	1% DSS treatment	1279:1294	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	4	50	theme	dextran	846:852	arg1	DSS					870:872	DSS	870:872	DSS	870:872	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	4	50	theme	dextran	846:852	arg1	sulfate					861:867	1% dextran sodium sulfate	843:867	1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial	843:922	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	8	51	theme	gene	1262:1265	arg1	sequencing					1267:1276	rRNA gene sequencing	1257:1276	rRNA gene sequencing	1257:1276	Fecal microbiota composition was assessed by 16S rRNA gene sequencing.
35939489	9	52	attach	presence	1384:1391	arg1	WD					1419:1420	WD	1419:1420	WD	1419:1420	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	9	52	attach	presence	1384:1391	arg2	fractions					1406:1414	faba bean fractions	1396:1414	faba bean fractions	1396:1414	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	1	53	theme	induced	145:151	arg1	inflammation					161:172	chemical induced colonic inflammation	136:172	chemical induced colonic inflammation	136:172	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
35939489	1	54	theme	bean	196:199	arg1	fractions					201:209	faba bean fractions	191:209	faba bean fractions	191:209	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
35939489	2	55	theme	fractions	303:311	arg1	role					277:280	the role	273:280	the role of protein and fiber fractions of faba beans for colonic health and microbiota composition	273:371	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	12	56	from	replacement	1797:1807	arg1	WD					1838:1839	a WD	1836:1839	a WD with faba bean fractions	1836:1864	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	10	57	theme	faba-bean	1543:1551	arg1	proteins					1553:1560	faba-bean proteins	1543:1560	faba-bean proteins	1543:1560	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	3	58	theme	C57BL/6JRj	472:481	arg1	mice					488:491	sixty C57BL/6JRj male mice	466:491	sixty C57BL/6JRj male mice	466:491	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	59	theme	plain	786:790	arg1	n					796:796	n = 24	796:801	n = 24	796:801	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	59	theme	plain	786:790	arg1	WD					792:793	4) plain WD	783:793	4) plain WD (n = 24)	783:802	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	11	60	theme	Actinobacteria	1635:1648	arg1	abundance					1650:1658	The Actinobacteria abundance	1631:1658	The Actinobacteria abundance	1631:1658	The Actinobacteria abundance was also lower in the group that had fiber fraction from faba-beans.
35939489	11	60	theme	Actinobacteria	1635:1648	arg1	lower					1669:1673	lower	1669:1673	lower	1669:1673	The Actinobacteria abundance was also lower in the group that had fiber fraction from faba-beans.
35939489	1	61	theme	chemical	136:143	arg1	inflammation					161:172	chemical induced colonic inflammation	136:172	chemical induced colonic inflammation	136:172	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
35939489	7	62	theme	Inflammatory	1080:1091	arg1	status					1093:1098	Inflammatory status	1080:1098	Inflammatory status in the colon	1080:1111	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	10	63	theme	lower	1568:1572	arg1	amount					1574:1579	a lower amount	1566:1579	a lower amount of Proteobacteria	1566:1597	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	10	63	theme	lower	1568:1572	arg1	Proteobacteria					1584:1597	Proteobacteria	1584:1597	Proteobacteria	1584:1597	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	1	64	theme	colonic	153:159	arg1	inflammation					161:172	chemical induced colonic inflammation	136:172	chemical induced colonic inflammation	136:172	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
35939489	9	65	theme	activity	1354:1361	arg1	score					1369:1373	disease activity index score	1346:1373	disease activity index score	1346:1373	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	3	66	theme	=	798:798	arg1	n					796:796	n = 24	796:801	n = 24	796:801	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	66	theme	=	798:798	arg1	WD					792:793	4) plain WD	783:793	4) plain WD (n = 24)	783:802	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	12	67	theme	low-grade	1767:1775	arg1	model					1790:1794	a low-grade inflammation model	1765:1794	a low-grade inflammation model	1765:1794	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	6	68	theme	body	999:1002	arg1	weight					1004:1009	body weight	999:1009	body weight	999:1009	Prior to DSS, body weight, energy intake, glucose and insulin tolerance assays were performed.
35939489	3	69	theme	mice	488:491	arg1	diet					458:461	The diet	454:461	The diet of sixty C57BL/6JRj male mice	454:491	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	9	70	theme	genes	1509:1513	arg1	level					1476:1480	the expression level	1461:1480	the expression level of inflammatory associated genes	1461:1513	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	3	71	with	WD	744:745	arg1	%					694:694	7%	693:694	7% of the fiber replaced with faba-bean fibers	693:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	71	with	WD	744:745	arg1	fractions					770:778	protein and fiber fractions	752:778	fractions	770:778	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	71	with	WD	744:745	arg1	fiber					703:707	the fiber	699:707	the fiber replaced with faba-bean fibers	699:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	72	with	proteins	672:679	arg1	%					694:694	7%	693:694	7% of the fiber replaced with faba-bean fibers	693:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	72	with	proteins	672:679	arg1	fractions					770:778	protein and fiber fractions	752:778	fractions	770:778	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	72	with	proteins	672:679	arg1	fiber					703:707	the fiber	699:707	the fiber replaced with faba-bean fibers	699:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	1	73	theme	Rodent	86:91	arg1	studies					93:99	Rodent studies	86:99	Rodent studies	86:99	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
35939489	9	74	from	levels	1306:1311	arg1	lipocalin-2					1322:1332	fecal lipocalin-2	1316:1332	fecal lipocalin-2	1316:1332	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	11	75	theme	fiber	1697:1701	arg1	fraction					1703:1710	fiber fraction	1697:1710	fiber fraction from faba-beans	1697:1726	The Actinobacteria abundance was also lower in the group that had fiber fraction from faba-beans.
35939489	4	76	theme	trial	918:922	arg1	days					906:909	the last six days	893:909	the last six days of the trial	893:922	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	10	77	theme	plain	1621:1625	arg1	WD					1627:1628	plain WD	1621:1628	plain WD	1621:1628	However, the mice that had faba-bean proteins had a lower amount of Proteobacteria compared the group on plain WD.
35939489	9	78	theme	faba	1396:1399	arg1	fractions					1406:1414	faba bean fractions	1396:1414	faba bean fractions	1396:1414	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	11	79	from	faba-beans	1717:1726	arg1	fraction					1703:1710	fiber fraction	1697:1710	fiber fraction from faba-beans	1697:1726	The Actinobacteria abundance was also lower in the group that had fiber fraction from faba-beans.
35939489	3	80	theme	%	521:521	arg1	fat					523:525	41% fat	519:525	41% fat	519:525	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	80	theme	%	521:521	arg1	carbohydrates					532:544	43% carbohydrates	528:544	43% carbohydrates	528:544	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	9	81	theme	fractions	1406:1414	arg1	presence					1384:1391	the presence	1380:1391	the presence of faba bean fractions in WD	1380:1420	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	3	82	theme	protein	640:646	arg1	%					631:631	30%	629:631	30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24)	629:802	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	82	theme	protein	640:646	arg1	protein					640:646	the protein	636:646	the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24)	636:802	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	0	83	theme	low	69:71	arg1	dose					73:76	low dose	69:76	low dose of DSS	69:83	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	3	84	dep	WD	744:745	arg1	3					741:741	3	741:741	3	741:741	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	85	theme	fiber	703:707	arg1	%					694:694	7%	693:694	7% of the fiber replaced with faba-bean fibers	693:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	85	theme	fiber	703:707	arg1	fiber					703:707	the fiber	699:707	the fiber replaced with faba-bean fibers	699:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	2	86	theme	Western	435:441	arg1	WD					449:450	WD	449:450	WD	449:450	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	2	86	theme	Western	435:441	arg1	diet					443:446	a Western diet	433:446	a Western diet (WD)	433:451	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	0	87	from	effect	11:16	arg1	mice					51:54	WD-fed mice	44:54	WD-fed mice	44:54	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	3	88	dep	WD	621:622	arg1	either					611:616	either	611:616	either	611:616	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	88	dep	WD	621:622	arg1	1					618:618	1	618:618	1	618:618	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	89	dep	WD	685:686	arg1	2					682:682	2	682:682	2	682:682	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	12	90	contain	had	1866:1868	arg1	replacement					1797:1807	replacement	1797:1807	replacement of protein and or fiber in a WD with faba bean fractions	1797:1864	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	12	90	contain	had	1866:1868	arg2	effects					1879:1885	marginal effects	1870:1885	marginal effects	1870:1885	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	12	91	theme	faba	1846:1849	arg1	fractions					1856:1864	faba bean fractions	1846:1864	faba bean fractions	1846:1864	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	3	92	with	WD	685:686	arg1	fractions					770:778	protein and fiber fractions	752:778	fractions	770:778	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	92	with	WD	685:686	arg1	%					694:694	7%	693:694	7% of the fiber replaced with faba-bean fibers	693:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	92	with	WD	685:686	arg1	fiber					703:707	the fiber	699:707	the fiber replaced with faba-bean fibers	699:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	12	93	theme	inflammatory	1890:1901	arg1	parameters					1903:1912	inflammatory parameters	1890:1912	inflammatory parameters	1890:1912	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	2	94	theme	colonic	331:337	arg1	health					339:344	colonic health	331:344	colonic health	331:344	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	8	95	theme	microbiota	1214:1223	arg1	composition					1225:1235	Fecal microbiota composition	1208:1235	Fecal microbiota composition	1208:1235	Fecal microbiota composition was assessed by 16S rRNA gene sequencing.
35939489	2	96	theme	beans	321:325	arg1	fractions					303:311	protein and fiber fractions	285:311	fractions	303:311	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	0	97	theme	faba	21:24	arg1	fractions					31:39	faba bean fractions	21:39	faba bean fractions	21:39	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	9	98	theme	expression	1465:1474	arg1	level					1476:1480	the expression level	1461:1480	the expression level of inflammatory associated genes	1461:1513	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	2	99	theme	faba	316:319	arg1	beans					321:325	faba beans	316:325	faba beans	316:325	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	0	100	theme	fractions	31:39	arg1	effect					11:16	Intestinal effect	0:16	Intestinal effect of faba bean fractions in WD-fed mice	0:54	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	3	101	theme	%	530:530	arg1	fat					523:525	41% fat	519:525	41% fat	519:525	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	101	theme	%	530:530	arg1	carbohydrates					532:544	43% carbohydrates	528:544	43% carbohydrates	528:544	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	12	102	theme	protein	1812:1818	arg1	replacement					1797:1807	replacement	1797:1807	replacement of protein and or fiber in a WD with faba bean fractions	1797:1864	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	0	103	theme	WD-fed	44:49	arg1	mice					51:54	WD-fed mice	44:54	WD-fed mice	44:54	Intestinal effect of faba bean fractions in WD-fed mice treated with low dose of DSS.
35939489	3	104	with	WD	792:793	arg1	%					694:694	7%	693:694	7% of the fiber replaced with faba-bean fibers	693:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	104	with	WD	792:793	arg1	fractions					770:778	protein and fiber fractions	752:778	fractions	770:778	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	3	104	with	WD	792:793	arg1	fiber					703:707	the fiber	699:707	the fiber replaced with faba-bean fibers	699:738	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	7	105	theme	related	1193:1199	arg1	genes					1201:1205	inflammatory related genes	1180:1205	inflammatory related genes	1180:1205	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	3	106	theme	fiber	764:768	arg1	fractions					770:778	protein and fiber fractions	752:778	fractions	770:778	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	9	107	theme	associated	1498:1507	arg1	genes					1509:1513	inflammatory associated genes	1485:1513	inflammatory associated genes	1485:1513	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	8	108	theme	rRNA	1257:1260	arg1	sequencing					1267:1276	rRNA gene sequencing	1257:1276	rRNA gene sequencing	1257:1276	Fecal microbiota composition was assessed by 16S rRNA gene sequencing.
35939489	12	109	theme	or	1824:1825	arg1	fiber					1827:1831	or fiber	1824:1831	or fiber	1824:1831	Overall, outcomes indicated that in a low-grade inflammation model, replacement of protein and or fiber in a WD with faba bean fractions had marginal effects on inflammatory parameters and colonic microbiota.
35939489	2	110	theme	protein	285:291	arg1	fractions					303:311	protein and fiber fractions	285:311	fractions	303:311	We have investigated the role of protein and fiber fractions of faba beans for colonic health and microbiota composition in a low-grade inflammation mice-model when incorporated in a Western diet (WD).
35939489	9	111	theme	%	1280:1280	arg1	treatment					1286:1294	1% DSS treatment	1279:1294	1% DSS treatment	1279:1294	1% DSS treatment increased levels in fecal lipocalin-2 and induced disease activity index score, but the presence of faba bean fractions in WD did not influence these indicators nor the expression level of inflammatory associated genes.
35939489	3	112	theme	protein	752:758	arg1	fractions					770:778	protein and fiber fractions	752:778	fractions	770:778	The diet of sixty C57BL/6JRj male mice was standardized to a WD (41% fat, 43% carbohydrates) before were randomly assigned to four groups (n = 12) receiving either 1) WD with 30% of the protein replaced with faba-bean proteins, 2) WD with 7% of the fiber replaced with faba-bean fibers, 3) WD with protein and fiber fractions or 4) plain WD (n = 24).
35939489	1	113	theme	faba	191:194	arg1	fractions					201:209	faba bean fractions	191:209	faba bean fractions	191:209	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
35939489	7	114	theme	qRT-PCR	1160:1166	arg1	analyses					1168:1175	qRT-PCR analyses	1160:1175	qRT-PCR analyses	1160:1175	Inflammatory status in the colon was assessed by biomarkers of inflammation and qRT-PCR analyses of inflammatory related genes.
35939489	4	115	theme	%	844:844	arg1	DSS					870:872	DSS	870:872	DSS	870:872	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	4	115	theme	%	844:844	arg1	sulfate					861:867	1% dextran sodium sulfate	843:867	1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial	843:922	Low-grade inflammation was induced by 1% dextran sodium sulfate (DSS) given to mice for the last six days of the trial.
35939489	1	116	theme	fractions	201:209	arg1	role					183:186	the role	179:186	the role of faba bean fractions for colon health	179:226	Rodent studies have shown that legumes can reduce chemical induced colonic inflammation, but the role of faba bean fractions for colon health has not been described.
36547553	4	0	from	weight	651:656	arg1	similar					614:620	similar	614:620	similar	614:620	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	1	1	dep	extent	91:96	arg1	The					87:89	The	87:89	The	87:89	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	6	2	theme	host	1109:1112	arg1	response					1114:1121	host response	1109:1121	host response	1109:1121	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	7	3	from	influence	1370:1378	arg1	FBR					1410:1412	FBR	1410:1412	FBR	1410:1412	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	2	4	theme	excellent	360:368	arg1	characteristics					398:412	their excellent polymeric and biocompatible characteristics	354:412	their excellent polymeric and biocompatible characteristics	354:412	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	4	5	theme	similar	614:620	arg1	glucomannans					600:611	two glucomannans	596:611	two glucomannans	596:611	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	7	6	dep	stimulate	1294:1302	arg1	providing					1418:1426	providing	1418:1426	providing clues for the fine regulation of immune response and novel biomaterials design	1418:1505	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	7	6	dep	stimulate	1294:1302	arg1	suggesting					1355:1364	suggesting	1355:1364	suggesting the influence of sugar chain arrangement on FBR	1355:1412	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	4	7	with	weight	651:656	arg1	differences					663:673	differences	663:673	differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP)	663:792	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	3	8	theme	biological	421:430	arg1	effects					432:438	Their biological effects	415:438	Their biological effects	415:438	Their biological effects are usually associated with their monosaccharide composition or functional groups, yet the contribution of their glycan structure is still unknown.
36547553	2	9	theme	diverse	291:297	arg1	biomaterials					299:310	diverse biomaterials design and biomedical applications	291:345	diverse biomaterials design and biomedical applications	291:345	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	5	10	theme	surface	1009:1015	arg1	morphology					1017:1026	surface morphology	1009:1026	surface morphology	1009:1026	After acetyl modification, these polysaccharides were fabricated into electrospun scaffolds to reduce the impacts derived from the physical properties and surface morphology.
36547553	6	11	theme	systematic	1044:1053	arg1	study					1055:1059	a systematic study	1042:1059	a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo	1042:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	4	12	from	composition	625:635	arg1	similar					614:620	similar	614:620	similar	614:620	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	6	13	theme	KGM	1197:1199	arg1	scaffolds					1209:1217	acetyl KGM (acKGM) scaffolds	1190:1217	acetyl KGM (acKGM) scaffolds	1190:1217	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	14	from	effects	1081:1087	arg1	cells					1099:1103	immune cells	1092:1103	immune cells	1092:1103	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	14	from	effects	1081:1087	arg1	response					1114:1121	host response	1109:1121	host response	1109:1121	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	2	15	theme	polymeric	370:378	arg1	characteristics					398:412	their excellent polymeric and biocompatible characteristics	354:412	their excellent polymeric and biocompatible characteristics	354:412	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	7	16	theme	immune	1461:1466	arg1	response					1468:1475	immune response	1461:1475	immune response	1461:1475	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	7	17	theme	chain	1389:1393	arg1	arrangement					1395:1405	sugar chain arrangement	1383:1405	sugar chain arrangement	1383:1405	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	3	18	theme	monosaccharide	474:487	arg1	composition					489:499	their monosaccharide composition	468:499	their monosaccharide composition	468:499	Their biological effects are usually associated with their monosaccharide composition or functional groups, yet the contribution of their glycan structure is still unknown.
36547553	4	19	from	similar	614:620	arg1	composition					625:635	composition	625:635	composition	625:635	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	19	from	similar	614:620	arg1	weight					651:656	molecular weight	641:656	molecular weight	641:656	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	7	20	theme	biomaterials	1487:1498	arg1	design					1500:1505	novel biomaterials design	1481:1505	novel biomaterials design	1481:1505	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	6	21	from	cells	1099:1103	arg1	study					1055:1059	a systematic study	1042:1059	a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo	1042:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	21	from	cells	1099:1103	arg1	model					1154:1158	a subcutaneous implantation model	1126:1158	a subcutaneous implantation model in vivo	1126:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	2	22	theme	increasing	267:276	arg1	attention					278:286	increasing attention	267:286	increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics	267:412	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	6	23	theme	implantation	1141:1152	arg1	model					1154:1158	a subcutaneous implantation model	1126:1158	a subcutaneous implantation model in vivo	1126:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	7	24	theme	sugar	1383:1387	arg1	arrangement					1395:1405	sugar chain arrangement	1383:1405	sugar chain arrangement	1383:1405	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	0	25	theme	Foreign	13:19	arg1	Reactions					26:34	Differential Foreign Body Reactions	0:34	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.	0:85	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.
36547553	7	26	theme	design	1500:1505	arg1	regulation					1447:1456	the fine regulation	1438:1456	the fine regulation of immune response and novel biomaterials design	1438:1505	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	2	27	theme	biomedical	323:332	arg1	applications					334:345	biomedical applications	323:345	biomedical applications	323:345	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	0	28	theme	Differential	0:11	arg1	Reactions					26:34	Differential Foreign Body Reactions	0:34	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.	0:85	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.
36547553	4	29	dep	polysaccharide	773:786	arg1	BSP					789:791	BSP	789:791	BSP	789:791	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	30	theme	striata	765:771	arg1	branched-chain					740:753	branched-chain	740:753	branched-chain (Bletilla striata polysaccharide, BSP)	740:792	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	30	theme	striata	765:771	arg1	polysaccharide					773:786	Bletilla striata polysaccharide	756:786	Bletilla striata polysaccharide	756:786	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	5	31	theme	electrospun	924:934	arg1	scaffolds					936:944	electrospun scaffolds	924:944	electrospun scaffolds	924:944	After acetyl modification, these polysaccharides were fabricated into electrospun scaffolds to reduce the impacts derived from the physical properties and surface morphology.
36547553	6	32	theme	stronger	1228:1235	arg1	FBR					1237:1239	a stronger FBR	1226:1239	a stronger FBR than acetyl BSP materials	1226:1265	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	33	dep	in	1160:1161	arg1	vivo					1163:1166	vivo	1163:1166	vivo	1163:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	34	theme	acetyl	1190:1195	arg1	KGM					1197:1199	acetyl KGM	1190:1199	acetyl KGM (acKGM) scaffolds	1190:1217	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	34	theme	acetyl	1190:1195	arg1	acKGM					1202:1206	acKGM	1202:1206	acKGM	1202:1206	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	1	35	theme	biomaterials	184:195	arg1	feasibility					169:179	feasibility	169:179	feasibility	169:179	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	1	35	theme	biomaterials	184:195	arg1	function					156:163	function	156:163	function	156:163	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	0	36	theme	Body	21:24	arg1	Reactions					26:34	Differential Foreign Body Reactions	0:34	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.	0:85	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.
36547553	3	37	theme	glycan	553:558	arg1	structure					560:568	their glycan structure	547:568	their glycan structure	547:568	Their biological effects are usually associated with their monosaccharide composition or functional groups, yet the contribution of their glycan structure is still unknown.
36547553	3	38	theme	structure	560:568	arg1	contribution					531:542	the contribution	527:542	the contribution of their glycan structure	527:568	Their biological effects are usually associated with their monosaccharide composition or functional groups, yet the contribution of their glycan structure is still unknown.
36547553	3	38	theme	structure	560:568	arg1	unknown					579:585	unknown	579:585	unknown	579:585	Their biological effects are usually associated with their monosaccharide composition or functional groups, yet the contribution of their glycan structure is still unknown.
36547553	0	39	theme	Branched	44:51	arg1	Scaffolds					76:84	Branched and Linear Glucomannan Scaffolds	44:84	Branched and Linear Glucomannan Scaffolds	44:84	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.
36547553	2	40	dep	biomaterials	299:310	arg1	design					312:317	design	312:317	design	312:317	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	2	40	dep	biomaterials	299:310	arg1	applications					334:345	biomedical applications	323:345	biomedical applications	323:345	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	6	41	theme	BSP	1253:1255	arg1	materials					1257:1265	acetyl BSP materials	1246:1265	acetyl BSP materials	1246:1265	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	2	42	theme	biomaterial	231:241	arg1	category					243:250	an important biomaterial category	218:250	an important biomaterial category	218:250	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	7	43	theme	novel	1481:1485	arg1	design					1500:1505	novel biomaterials design	1481:1505	novel biomaterials design	1481:1505	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	7	44	theme	pro-inflammatory	1327:1342	arg1	cytokines					1344:1352	pro-inflammatory cytokines	1327:1352	pro-inflammatory cytokines	1327:1352	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	6	45	theme	subcutaneous	1128:1139	arg1	model					1154:1158	a subcutaneous implantation model	1126:1158	a subcutaneous implantation model in vivo	1126:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	2	46	theme	important	221:229	arg1	category					243:250	an important biomaterial category	218:250	an important biomaterial category	218:250	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	3	47	theme	functional	504:513	arg1	groups					515:520	functional groups	504:520	functional groups	504:520	Their biological effects are usually associated with their monosaccharide composition or functional groups, yet the contribution of their glycan structure is still unknown.
36547553	0	48	theme	Linear	57:62	arg1	Scaffolds					76:84	Branched and Linear Glucomannan Scaffolds	44:84	Branched and Linear Glucomannan Scaffolds	44:84	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.
36547553	1	49	dep	function	156:163	arg1	the					152:154	the	152:154	the	152:154	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	1	50	theme	foreign	114:120	arg1	reaction					127:134	foreign body reaction	114:134	foreign body reaction (FBR)	114:140	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	1	50	theme	foreign	114:120	arg1	FBR					137:139	FBR	137:139	FBR	137:139	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	4	51	theme	molecular	641:649	arg1	weight					651:656	molecular weight	641:656	molecular weight	641:656	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	6	52	from	study	1055:1059	arg1	cells					1099:1103	immune cells	1092:1103	immune cells	1092:1103	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	52	from	study	1055:1059	arg1	response					1114:1121	host response	1109:1121	host response	1109:1121	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	53	theme	acetyl	1246:1251	arg1	materials					1257:1265	acetyl BSP materials	1246:1265	acetyl BSP materials	1246:1265	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	1	54	theme	body	122:125	arg1	reaction					127:134	foreign body reaction	114:134	foreign body reaction (FBR)	114:140	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	1	54	theme	body	122:125	arg1	FBR					137:139	FBR	137:139	FBR	137:139	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	2	55	from	attention	278:286	arg1	biomaterials					299:310	diverse biomaterials design and biomedical applications	291:345	diverse biomaterials design and biomedical applications	291:345	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	7	56	theme	arrangement	1395:1405	arg1	influence					1370:1378	the influence	1366:1378	the influence of sugar chain arrangement on FBR	1366:1412	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	5	57	theme	physical	985:992	arg1	properties					994:1003	the physical properties	981:1003	the physical properties	981:1003	After acetyl modification, these polysaccharides were fabricated into electrospun scaffolds to reduce the impacts derived from the physical properties and surface morphology.
36547553	4	58	dep	glucomannan	717:727	arg1	KGM					730:732	KGM	730:732	KGM	730:732	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	6	59	theme	effects	1081:1087	arg1	study					1055:1059	a systematic study	1042:1059	a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo	1042:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	1	60	theme	reaction	127:134	arg1	patterns					102:109	patterns	102:109	patterns	102:109	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	1	60	theme	reaction	127:134	arg1	extent					91:96	extent	91:96	extent	91:96	The extent and patterns of foreign body reaction (FBR) influence the function and feasibility of biomaterials.
36547553	7	61	theme	fine	1442:1445	arg1	regulation					1447:1456	the fine regulation	1438:1456	the fine regulation of immune response and novel biomaterials design	1438:1505	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	7	62	theme	response	1468:1475	arg1	regulation					1447:1456	the fine regulation	1438:1456	the fine regulation of immune response and novel biomaterials design	1438:1505	Additionally, acKGM could stimulate macrophages to release pro-inflammatory cytokines, suggesting the influence of sugar chain arrangement on FBR and providing clues for the fine regulation of immune response and novel biomaterials design.
36547553	4	63	with	composition	625:635	arg1	differences					663:673	differences	663:673	differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP)	663:792	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	64	theme	Bletilla	756:763	arg1	branched-chain					740:753	branched-chain	740:753	branched-chain (Bletilla striata polysaccharide, BSP)	740:792	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	64	theme	Bletilla	756:763	arg1	polysaccharide					773:786	Bletilla striata polysaccharide	756:786	Bletilla striata polysaccharide	756:786	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	65	from	differences	663:673	arg1	structure					685:693	glycan structure	678:693	glycan structure	678:693	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	65	from	differences	663:673	arg1	branched-chain					740:753	branched-chain	740:753	branched-chain (Bletilla striata polysaccharide, BSP)	740:792	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	65	from	differences	663:673	arg1	polysaccharide					773:786	Bletilla striata polysaccharide	756:786	Bletilla striata polysaccharide	756:786	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	65	from	differences	663:673	arg1	linear-chain					696:707	linear-chain	696:707	linear-chain (Konjac glucomannan, KGM)	696:733	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	65	from	differences	663:673	arg1	glucomannan					717:727	glucomannan	717:727	glucomannan	717:727	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	6	66	theme	immune	1092:1097	arg1	cells					1099:1103	immune cells	1092:1103	immune cells	1092:1103	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	4	67	theme	host-biomaterials	823:839	arg1	interaction					841:851	the host-biomaterials interaction	819:851	the host-biomaterials interaction	819:851	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	4	68	theme	glycan	678:683	arg1	structure					685:693	glycan structure	678:693	glycan structure	678:693	Herein, two glucomannans, similar in composition and molecular weight with differences in glycan structure, linear-chain (Konjac glucomannan, KGM), and branched-chain (Bletilla striata polysaccharide, BSP), were adopted to explore the host-biomaterials interaction.
36547553	2	69	theme	biocompatible	384:396	arg1	characteristics					398:412	their excellent polymeric and biocompatible characteristics	354:412	their excellent polymeric and biocompatible characteristics	354:412	Polysaccharides, as an important biomaterial category, have received increasing attention in diverse biomaterials design and biomedical applications due to their excellent polymeric and biocompatible characteristics.
36547553	6	70	theme	biological	1070:1079	arg1	effects					1081:1087	their biological effects	1064:1087	their biological effects on immune cells and host response in a subcutaneous implantation model in vivo	1064:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	0	71	theme	Glucomannan	64:74	arg1	Scaffolds					76:84	Branched and Linear Glucomannan Scaffolds	44:84	Branched and Linear Glucomannan Scaffolds	44:84	Differential Foreign Body Reactions between Branched and Linear Glucomannan Scaffolds.
36547553	5	72	attach	derived	968:974	arg2	impacts					960:966	the impacts	956:966	the impacts derived from the physical properties and surface morphology	956:1026	After acetyl modification, these polysaccharides were fabricated into electrospun scaffolds to reduce the impacts derived from the physical properties and surface morphology.
36547553	5	72	attach	derived	968:974	arg1	morphology					1017:1026	surface morphology	1009:1026	surface morphology	1009:1026	After acetyl modification, these polysaccharides were fabricated into electrospun scaffolds to reduce the impacts derived from the physical properties and surface morphology.
36547553	5	72	attach	derived	968:974	arg1	properties					994:1003	the physical properties	981:1003	the physical properties	981:1003	After acetyl modification, these polysaccharides were fabricated into electrospun scaffolds to reduce the impacts derived from the physical properties and surface morphology.
36547553	5	73	theme	acetyl	860:865	arg1	modification					867:878	acetyl modification	860:878	acetyl modification	860:878	After acetyl modification, these polysaccharides were fabricated into electrospun scaffolds to reduce the impacts derived from the physical properties and surface morphology.
36547553	6	74	from	response	1114:1121	arg1	study					1055:1059	a systematic study	1042:1059	a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo	1042:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	74	from	response	1114:1121	arg1	model					1154:1158	a subcutaneous implantation model	1126:1158	a subcutaneous implantation model in vivo	1126:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
36547553	6	75	theme	in	1160:1161	arg1	model					1154:1158	a subcutaneous implantation model	1126:1158	a subcutaneous implantation model in vivo	1126:1166	According to a systematic study of their biological effects on immune cells and host response in a subcutaneous implantation model in vivo, it was revealed that acetyl KGM (acKGM) scaffolds caused a stronger FBR than acetyl BSP materials.
35961187	4	0	theme	peptide	878:884	arg1	release					864:870	a burst release	856:870	a burst release of E7 peptide	856:884	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	1	theme	peptide	1030:1036	arg1	release					1015:1021	a sustained release	1003:1021	a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h	1003:1101	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	3	2	theme	400 μm	714:719	arg1	pores					705:709	lower pores	699:709	lower pores of 400 μm for bone regeneration	699:741	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	3	2	theme	400 μm	714:719	arg1	pores					652:656	upper pores	646:656	upper pores of 200 μm for cartilage regeneration	646:693	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	6	3	theme	osteochondral	1500:1512	arg1	model					1521:1525	rabbit osteochondral defect model	1493:1525	rabbit osteochondral defect model	1493:1525	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	5	4	theme	good	1203:1206	arg1	biocompatibility					1208:1223	good biocompatibility	1203:1223	good biocompatibility	1203:1223	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	4	5	theme	burst	858:862	arg1	release					864:870	a burst release	856:870	a burst release of E7 peptide	856:884	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	5	6	theme	BE-PSA	1183:1188	arg1	scaffold					1190:1197	The BE-PSA scaffold	1179:1197	The BE-PSA scaffold	1179:1197	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	1	7	link	marrow-derived	263:276	arg1	cells					295:299	bone marrow-derived mesenchymal stem cells	258:299	bone marrow-derived mesenchymal stem cells (BMSCs)	258:307	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	1	7	link	marrow-derived	263:276	arg1	BMSCs					302:306	BMSCs	302:306	BMSCs	302:306	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	4	8	theme	degradation	1131:1141	arg1	rates					1143:1147	the different degradation rates	1117:1147	the different degradation rates of SA hydrogel and SF matrix	1117:1176	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	3	9	theme	polycaprolactone	605:620	arg1	scaffold					628:635	The 3D-printed polycaprolactone (PCL) scaffold	590:635	The 3D-printed polycaprolactone (PCL) scaffold	590:635	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	7	10	theme	biomaterials	1699:1710	arg1	fabrication					1665:1675	the fabrication	1661:1675	the fabrication of tissue engineering biomaterials based on the structural and biochemical modification	1661:1763	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	5	11	dep	migration	1247:1255	arg1	the					1243:1245	the	1243:1245	the	1243:1245	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	0	12	theme	osteochondral	122:134	arg1	regeneration					136:147	osteochondral regeneration	122:147	osteochondral regeneration	122:147	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	7	13	theme	tissue	1680:1685	arg1	biomaterials					1699:1710	tissue engineering biomaterials	1680:1710	tissue engineering biomaterials based on the structural and biochemical modification	1680:1763	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	4	14	theme	slowly-degrading	934:949	arg1	SF					965:966	SF	965:966	SF	965:966	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	14	theme	slowly-degrading	934:949	arg1	fibroin					956:962	slowly-degrading silk fibroin	934:962	a slowly-degrading silk fibroin (SF) porous matrix	932:981	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	15	theme	SF	1168:1169	arg1	matrix					1171:1176	SF matrix	1168:1176	SF matrix	1168:1176	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	16	theme	sequential	750:759	arg1	modulation					761:770	a sequential modulation	748:770	a sequential modulation of BMSCs behavior	748:788	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	17	theme	porous	969:974	arg1	matrix					976:981	a slowly-degrading silk fibroin (SF) porous matrix	932:981	a slowly-degrading silk fibroin (SF) porous matrix	932:981	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	6	18	theme	BE-PSA	1414:1419	arg1	scaffold					1421:1428	the BE-PSA scaffold	1410:1428	the BE-PSA scaffold	1410:1428	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	3	19	theme	upper	646:650	arg1	pores					652:656	upper pores	646:656	upper pores of 200 μm for cartilage regeneration	646:693	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	2	20	theme	fused	507:511	arg1	printing					542:549	fused deposition modeling (FDM) 3D printing	507:549	fused deposition modeling (FDM) 3D printing	507:549	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	1	21	dep	cartilage	322:330	arg1	regeneration					353:364	regeneration	353:364	regeneration	353:364	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	4	22	theme	hydrogel	1155:1162	arg1	rates					1143:1147	the different degradation rates	1117:1147	the different degradation rates of SA hydrogel and SF matrix	1117:1176	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	1	23	with	scaffolds	183:191	arg1	cues					230:233	both structural and biochemical cues	198:233	both structural and biochemical cues	198:233	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	1	24	theme	scaffolds	183:191	arg1	challenge					377:385	a challenge	375:385	a challenge	375:385	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	1	24	theme	scaffolds	183:191	arg1	fabrication					154:164	The fabrication	150:164	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration	150:364	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	2	25	theme	3D	539:540	arg1	printing					542:549	fused deposition modeling (FDM) 3D printing	507:549	fused deposition modeling (FDM) 3D printing	507:549	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	4	26	theme	fast-degrading	791:804	arg1	hydrogel					827:834	fast-degrading sodium alginate (SA) hydrogel	791:834	fast-degrading sodium alginate (SA) hydrogel	791:834	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	5	27	theme	osteogenic/chondrogenic	1261:1283	arg1	differentiation					1285:1299	osteogenic/chondrogenic differentiation	1261:1299	osteogenic/chondrogenic differentiation	1261:1299	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	6	28	theme	biomolecule	1388:1398	arg1	delivery					1400:1407	programmed biomolecule delivery	1377:1407	programmed biomolecule delivery	1377:1407	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	2	29	theme	FDM	534:536	arg1	printing					542:549	fused deposition modeling (FDM) 3D printing	507:549	fused deposition modeling (FDM) 3D printing	507:549	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	4	30	theme	alginate	813:820	arg1	hydrogel					827:834	fast-degrading sodium alginate (SA) hydrogel	791:834	fast-degrading sodium alginate (SA) hydrogel	791:834	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	31	used	used	987:990	arg2	matrix					976:981	a slowly-degrading silk fibroin (SF) porous matrix	932:981	a slowly-degrading silk fibroin (SF) porous matrix	932:981	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	2	32	theme	programmed	459:468	arg1	delivery					482:489	programmed biomolecule delivery	459:489	programmed biomolecule delivery	459:489	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	2	33	theme	modeling	524:531	arg1	printing					542:549	fused deposition modeling (FDM) 3D printing	507:549	fused deposition modeling (FDM) 3D printing	507:549	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	0	34	theme	3D-printed	0:9	arg1	scaffold					21:28	3D-printed composite scaffold	0:28	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery	0:88	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	4	35	theme	B2A	1026:1028	arg1	peptide					1030:1036	B2A peptide	1026:1036	B2A peptide	1026:1036	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	36	theme	behavior	781:788	arg1	modulation					761:770	a sequential modulation	748:770	a sequential modulation of BMSCs behavior	748:788	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	6	37	theme	subchondral	1460:1470	arg1	bone					1472:1475	subchondral bone	1460:1475	subchondral bone	1460:1475	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	7	38	theme	guide	1580:1584	arg1	behavior					1592:1599	guide BMSCs behavior	1580:1599	guide BMSCs behavior	1580:1599	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	0	39	theme	gradient	35:42	arg1	structure					44:52	gradient structure	35:52	gradient structure	35:52	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	6	40	theme	cartilage	1446:1454	arg1	regeneration					1477:1488	enhanced cartilage and subchondral bone regeneration	1437:1488	enhanced cartilage and subchondral bone regeneration	1437:1488	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	0	41	theme	biomolecule	69:79	arg1	delivery					81:88	programmed biomolecule delivery	58:88	programmed biomolecule delivery	58:88	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	0	42	with	scaffold	21:28	arg1	structure					44:52	gradient structure	35:52	gradient structure	35:52	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	0	42	with	scaffold	21:28	arg1	delivery					81:88	programmed biomolecule delivery	58:88	programmed biomolecule delivery	58:88	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	4	43	theme	SA	823:824	arg1	hydrogel					827:834	fast-degrading sodium alginate (SA) hydrogel	791:834	fast-degrading sodium alginate (SA) hydrogel	791:834	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	1	44	theme	endogenous	247:256	arg1	behavior					309:316	endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior	247:316	endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration	247:364	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	3	45	theme	lower	699:703	arg1	pores					705:709	lower pores	699:709	lower pores of 400 μm for bone regeneration	699:741	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	1	46	theme	marrow-derived	263:276	arg1	cells					295:299	bone marrow-derived mesenchymal stem cells	258:299	bone marrow-derived mesenchymal stem cells (BMSCs)	258:307	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	1	46	theme	marrow-derived	263:276	arg1	BMSCs					302:306	BMSCs	302:306	BMSCs	302:306	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	6	47	theme	enhanced	1437:1444	arg1	regeneration					1477:1488	enhanced cartilage and subchondral bone regeneration	1437:1488	enhanced cartilage and subchondral bone regeneration	1437:1488	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	6	48	theme	defect	1514:1519	arg1	model					1521:1525	rabbit osteochondral defect model	1493:1525	rabbit osteochondral defect model	1493:1525	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	1	49	theme	stem	290:293	arg1	cells					295:299	bone marrow-derived mesenchymal stem cells	258:299	bone marrow-derived mesenchymal stem cells (BMSCs)	258:307	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	1	49	theme	stem	290:293	arg1	BMSCs					302:306	BMSCs	302:306	BMSCs	302:306	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	2	50	with	scaffold	413:420	arg1	delivery					482:489	programmed biomolecule delivery	459:489	programmed biomolecule delivery	459:489	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	2	50	with	scaffold	413:420	arg1	structure					445:453	gradient structure	436:453	gradient structure	436:453	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	4	51	theme	E7	875:876	arg1	peptide					878:884	E7 peptide	875:884	E7 peptide	875:884	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	52	theme	BMSCs	1049:1053	arg1	differentiation					1068:1082	BMSCs dual-lineage differentiation	1049:1082	BMSCs dual-lineage differentiation	1049:1082	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	6	53	theme	rabbit	1493:1498	arg1	model					1521:1525	rabbit osteochondral defect model	1493:1525	rabbit osteochondral defect model	1493:1525	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	4	54	theme	different	1121:1129	arg1	rates					1143:1147	the different degradation rates	1117:1147	the different degradation rates of SA hydrogel and SF matrix	1117:1176	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	5	55	contain	had	1199:1201	arg1	scaffold					1190:1197	The BE-PSA scaffold	1179:1197	The BE-PSA scaffold	1179:1197	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	5	55	contain	had	1199:1201	arg2	biocompatibility					1208:1223	good biocompatibility	1203:1223	good biocompatibility	1203:1223	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	3	56	theme	3D-printed	594:603	arg1	PCL					623:625	PCL	623:625	PCL	623:625	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	3	56	theme	3D-printed	594:603	arg1	polycaprolactone					605:620	The 3D-printed polycaprolactone	590:620	The 3D-printed polycaprolactone (PCL) scaffold	590:635	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	4	57	used	used	840:843	arg2	hydrogel					827:834	fast-degrading sodium alginate (SA) hydrogel	791:834	fast-degrading sodium alginate (SA) hydrogel	791:834	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	1	58	theme	structural	203:212	arg1	cues					230:233	both structural and biochemical cues	198:233	both structural and biochemical cues	198:233	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	7	59	theme	engineering	1687:1697	arg1	biomaterials					1699:1710	tissue engineering biomaterials	1680:1710	tissue engineering biomaterials based on the structural and biochemical modification	1680:1763	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	6	60	theme	structures	1362:1371	arg1	effects					1343:1349	the synergistic effects	1327:1349	the synergistic effects of spatial structures and programmed biomolecule delivery	1327:1407	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	1	61	theme	biochemical	218:228	arg1	cues					230:233	both structural and biochemical cues	198:233	both structural and biochemical cues	198:233	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	2	62	theme	multi-material-based	555:574	arg1	modification					576:587	multi-material-based modification	555:587	multi-material-based modification	555:587	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	1	63	dep	endogenous	247:256	arg1	cells					295:299	bone marrow-derived mesenchymal stem cells	258:299	bone marrow-derived mesenchymal stem cells (BMSCs)	258:307	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	1	63	dep	endogenous	247:256	arg1	BMSCs					302:306	BMSCs	302:306	BMSCs	302:306	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	4	64	theme	matrix	1171:1176	arg1	rates					1143:1147	the different degradation rates	1117:1147	the different degradation rates of SA hydrogel and SF matrix	1117:1176	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	6	65	theme	delivery	1400:1407	arg1	effects					1343:1349	the synergistic effects	1327:1349	the synergistic effects of spatial structures and programmed biomolecule delivery	1327:1407	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	6	66	theme	synergistic	1331:1341	arg1	effects					1343:1349	the synergistic effects	1327:1349	the synergistic effects of spatial structures and programmed biomolecule delivery	1327:1407	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	4	67	theme	BMSCs	897:901	arg1	migration					903:911	BMSCs migration	897:911	BMSCs migration	897:911	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	68	theme	SA	1152:1153	arg1	hydrogel					1155:1162	SA hydrogel	1152:1162	SA hydrogel	1152:1162	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	5	69	theme	BMSCs	1304:1308	arg1	migration					1247:1255	migration	1247:1255	migration	1247:1255	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	5	69	theme	BMSCs	1304:1308	arg1	differentiation					1285:1299	osteogenic/chondrogenic differentiation	1261:1299	osteogenic/chondrogenic differentiation	1261:1299	The BE-PSA scaffold had good biocompatibility and could improve the migration and osteogenic/chondrogenic differentiation of BMSCs.
35961187	2	70	theme	deposition	513:522	arg1	printing					542:549	fused deposition modeling (FDM) 3D printing	507:549	fused deposition modeling (FDM) 3D printing	507:549	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	0	71	theme	stem	99:102	arg1	behavior					109:116	stem cell behavior	99:116	stem cell behavior for osteochondral regeneration	99:147	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	1	72	theme	osteochondral	169:181	arg1	scaffolds					183:191	osteochondral scaffolds	169:191	osteochondral scaffolds with both structural and biochemical cues	169:233	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	7	73	theme	osteochondral	1605:1617	arg1	regeneration					1619:1630	osteochondral regeneration	1605:1630	osteochondral regeneration	1605:1630	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	6	74	theme	spatial	1354:1360	arg1	structures					1362:1371	spatial structures	1354:1371	spatial structures	1354:1371	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	0	75	theme	cell	104:107	arg1	behavior					109:116	stem cell behavior	99:116	stem cell behavior for osteochondral regeneration	99:147	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	4	76	theme	silk	951:954	arg1	SF					965:966	SF	965:966	SF	965:966	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	4	76	theme	silk	951:954	arg1	fibroin					956:962	slowly-degrading silk fibroin	934:962	a slowly-degrading silk fibroin (SF) porous matrix	932:981	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	6	77	theme	programmed	1377:1386	arg1	delivery					1400:1407	programmed biomolecule delivery	1377:1407	programmed biomolecule delivery	1377:1407	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	4	78	theme	sodium	806:811	arg1	hydrogel					827:834	fast-degrading sodium alginate (SA) hydrogel	791:834	fast-degrading sodium alginate (SA) hydrogel	791:834	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	2	79	theme	composite	403:411	arg1	BE-PSA					423:428	BE-PSA	423:428	BE-PSA	423:428	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	2	79	theme	composite	403:411	arg1	scaffold					413:420	a composite scaffold	401:420	a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery	401:489	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	0	80	theme	composite	11:19	arg1	scaffold					21:28	3D-printed composite scaffold	0:28	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery	0:88	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	4	81	theme	fibroin	956:962	arg1	matrix					976:981	a slowly-degrading silk fibroin (SF) porous matrix	932:981	a slowly-degrading silk fibroin (SF) porous matrix	932:981	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	7	82	theme	biochemical	1740:1750	arg1	modification					1752:1763	the structural and biochemical modification	1721:1763	the structural and biochemical modification	1721:1763	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	4	83	theme	sustained	1005:1013	arg1	release					1015:1021	a sustained release	1003:1021	a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h	1003:1101	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	6	84	theme	bone	1472:1475	arg1	regeneration					1477:1488	enhanced cartilage and subchondral bone regeneration	1437:1488	enhanced cartilage and subchondral bone regeneration	1437:1488	Benefiting from the synergistic effects of spatial structures and programmed biomolecule delivery, the BE-PSA scaffold showed enhanced cartilage and subchondral bone regeneration in rabbit osteochondral defect model.
35961187	7	85	theme	structural	1725:1734	arg1	modification					1752:1763	the structural and biochemical modification	1721:1763	the structural and biochemical modification	1721:1763	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	2	86	theme	gradient	436:443	arg1	structure					445:453	gradient structure	436:453	gradient structure	436:453	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	3	87	theme	cartilage	672:680	arg1	regeneration					682:693	cartilage regeneration	672:693	cartilage regeneration	672:693	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	4	88	theme	BMSCs	775:779	arg1	behavior					781:788	BMSCs behavior	775:788	BMSCs behavior	775:788	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	7	89	theme	BMSCs	1586:1590	arg1	behavior					1592:1599	guide BMSCs behavior	1580:1599	guide BMSCs behavior	1580:1599	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	3	90	theme	200 μm	661:666	arg1	pores					705:709	lower pores	699:709	lower pores of 400 μm for bone regeneration	699:741	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	3	90	theme	200 μm	661:666	arg1	pores					652:656	upper pores	646:656	upper pores of 200 μm for cartilage regeneration	646:693	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	0	91	theme	programmed	58:67	arg1	delivery					81:88	programmed biomolecule delivery	58:88	programmed biomolecule delivery	58:88	3D-printed composite scaffold with gradient structure and programmed biomolecule delivery to guide stem cell behavior for osteochondral regeneration.
35961187	1	92	theme	subchondral	336:346	arg1	bone					348:351	subchondral bone	336:351	subchondral bone	336:351	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	2	93	theme	biomolecule	470:480	arg1	delivery					482:489	programmed biomolecule delivery	459:489	programmed biomolecule delivery	459:489	To this end, a composite scaffold (BE-PSA) with gradient structure and programmed biomolecule delivery was prepared by fused deposition modeling (FDM) 3D printing and multi-material-based modification.
35961187	7	94	theme	promising	1558:1566	arg1	scaffold					1568:1575	a promising scaffold	1556:1575	a promising scaffold to guide BMSCs behavior for osteochondral regeneration	1556:1630	This work not only provides a promising scaffold to guide BMSCs behavior for osteochondral regeneration but also offers a method for the fabrication of tissue engineering biomaterials based on the structural and biochemical modification.
35961187	4	95	theme	dual-lineage	1055:1066	arg1	differentiation					1068:1082	BMSCs dual-lineage differentiation	1049:1082	BMSCs dual-lineage differentiation	1049:1082	For a sequential modulation of BMSCs behavior, fast-degrading sodium alginate (SA) hydrogel was used to deliver a burst release of E7 peptide to enhance BMSCs migration within 72 h, while a slowly-degrading silk fibroin (SF) porous matrix was used to provide a sustained release of B2A peptide to improve BMSCs dual-lineage differentiation lasting for >300 h, depending on the different degradation rates of SA hydrogel and SF matrix.
35961187	3	96	theme	bone	725:728	arg1	regeneration					730:741	bone regeneration	725:741	bone regeneration	725:741	The 3D-printed polycaprolactone (PCL) scaffold included upper pores of 200 μm for cartilage regeneration and lower pores of 400 μm for bone regeneration.
35961187	1	97	theme	mesenchymal	278:288	arg1	cells					295:299	bone marrow-derived mesenchymal stem cells	258:299	bone marrow-derived mesenchymal stem cells (BMSCs)	258:307	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
35961187	1	97	theme	mesenchymal	278:288	arg1	BMSCs					302:306	BMSCs	302:306	BMSCs	302:306	The fabrication of osteochondral scaffolds with both structural and biochemical cues to regulate endogenous bone marrow-derived mesenchymal stem cells (BMSCs) behavior for cartilage and subchondral bone regeneration is still a challenge.
36153996	6	0	theme	health	1308:1313	arg1	targets					1315:1321	intestinal health targets	1297:1321	intestinal health targets	1297:1321	However, high concentrations of CNM induced serious adverse effects, negatively improving intestinal health targets.
36153996	3	1	theme	bioactive	548:556	arg1	peptides					558:565	bioactive peptides	548:565	bioactive peptides	548:565	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	0	2	dep	Antimicrobials	95:108	arg1	Antimicrobials					95:108	Novel Antimicrobials	89:108	Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157	89:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	2	dep	Antimicrobials	95:108	arg1	Assessment					141:150	Risk Assessment	136:150	Risk Assessment	136:150	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	2	dep	Antimicrobials	95:108	arg1	O157					197:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	5	3	theme	CNM	922:924	arg1	levels					912:917	low levels	908:917	low levels of CNM	908:924	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	2	4	theme	severe	437:442	arg1	threat					451:456	a severe global threat	435:456	a severe global threat to public health	435:473	Infections by zoonotic enterohemorrhagic Escherichia coli are a severe global threat to public health.
36153996	2	4	theme	severe	437:442	arg1	Infections					373:382	Infections	373:382	Infections by zoonotic enterohemorrhagic Escherichia coli	373:429	Infections by zoonotic enterohemorrhagic Escherichia coli are a severe global threat to public health.
36153996	4	5	theme	CNM	700:702	arg1	assessment					686:695	the risk assessment	677:695	the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts	677:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	0	6	theme	Immune	218:223	arg1	Regulation					225:234	Immune Regulation	218:234	Immune Regulation	218:234	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	4	7	from	CNM	700:702	arg1	modulation					745:754	its potential immune modulation	724:754	its potential immune modulation	724:754	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	4	7	from	CNM	700:702	arg1	health					714:719	animal health	707:719	animal health	707:719	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	7	8	dep	E.	1403:1404	arg1	coli					1406:1409	coli	1406:1409	coli	1406:1409	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	4	9	theme	animal	707:712	arg1	health					714:719	animal health	707:719	animal health	707:719	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	6	10	theme	adverse	1259:1265	arg1	effects					1267:1273	serious adverse effects	1251:1273	serious adverse effects	1251:1273	However, high concentrations of CNM induced serious adverse effects, negatively improving intestinal health targets.
36153996	4	11	from	assessment	686:695	arg1	modulation					745:754	its potential immune modulation	724:754	its potential immune modulation	724:754	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	4	11	from	assessment	686:695	arg1	health					714:719	animal health	707:719	animal health	707:719	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	7	12	dep	O157	1411:1414	arg1	H7					1416:1417	H7	1416:1417	E. coli O157:H7 infection	1403:1427	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	3	13	used	used	602:605	arg2	class					521:525	a class	519:525	a class of APs engineered by bioactive peptides and chitosan nanoparticles	519:592	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	13	used	used	602:605	arg2	agent					632:636	a novel antimicrobial agent	610:636	a novel antimicrobial agent against bacterial infections	610:665	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	13	used	used	602:605	arg2	CNM					513:515	CNM	513:515	CNM	513:515	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	13	used	used	602:605	arg2	J25					508:510	Chitosan nanoparticles-microcin J25	476:510	Chitosan nanoparticles-microcin J25 (CNM)	476:516	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	0	14	theme	Risk	136:139	arg1	Antimicrobials					95:108	Novel Antimicrobials	89:108	Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157	89:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	14	theme	Risk	136:139	arg1	Assessment					141:150	Risk Assessment	136:150	Risk Assessment	136:150	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	6	15	theme	serious	1251:1257	arg1	effects					1267:1273	serious adverse effects	1251:1273	serious adverse effects	1251:1273	However, high concentrations of CNM induced serious adverse effects, negatively improving intestinal health targets.
36153996	1	16	theme	Polymeric	237:245	arg1	APs					262:264	APs	262:264	APs	262:264	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	1	16	theme	Polymeric	237:245	arg1	nanomaterials					247:259	Polymeric nanomaterials	237:259	Polymeric nanomaterials (APs)	237:265	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	6	17	theme	CNM	1239:1241	arg1	concentrations					1221:1234	high concentrations	1216:1234	high concentrations of CNM	1216:1241	However, high concentrations of CNM induced serious adverse effects, negatively improving intestinal health targets.
36153996	0	18	theme	Pathogen	171:178	arg1	Antimicrobials					95:108	Novel Antimicrobials	89:108	Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157	89:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	18	theme	Pathogen	171:178	arg1	O157					197:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	5	19	theme	significant	1046:1056	arg1	improvement					1058:1068	a significant improvement	1044:1068	a significant improvement in gut health	1044:1082	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	3	20	theme	antimicrobial	618:630	arg1	agent					632:636	a novel antimicrobial agent	610:636	a novel antimicrobial agent against bacterial infections	610:665	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	20	theme	antimicrobial	618:630	arg1	J25					508:510	Chitosan nanoparticles-microcin J25	476:510	Chitosan nanoparticles-microcin J25 (CNM)	476:516	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	5	21	from	findings	845:852	arg1	models					863:868	mouse models	857:868	mouse models	857:868	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	6	22	theme	intestinal	1297:1306	arg1	targets					1315:1321	intestinal health targets	1297:1321	intestinal health targets	1297:1321	However, high concentrations of CNM induced serious adverse effects, negatively improving intestinal health targets.
36153996	7	23	theme	host	1382:1385	arg1	defense					1387:1393	host defense	1382:1393	host defense against E. coli O157:H7 infection	1382:1427	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	5	24	from	effects	986:992	arg1	lifespan					1001:1008	the lifespan	997:1008	the lifespan	997:1008	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	24	from	effects	986:992	arg1	signs					1022:1026	clinical signs	1013:1026	clinical signs	1013:1026	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	25	theme	immune	1129:1134	arg1	modulation					1136:1145	immune modulation	1129:1145	immune modulation	1129:1145	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	26	theme	low	908:910	arg1	levels					912:917	low levels	908:917	low levels of CNM	908:924	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	4	27	theme	potential	728:736	arg1	modulation					745:754	its potential immune modulation	724:754	its potential immune modulation	724:754	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	0	28	theme	Novel	89:93	arg1	Antimicrobials					95:108	Novel Antimicrobials	89:108	Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157	89:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	28	theme	Novel	89:93	arg1	Assessment					141:150	Risk Assessment	136:150	Risk Assessment	136:150	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	28	theme	Novel	89:93	arg1	O157					197:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	9	29	theme	host	1792:1795	arg1	immunity					1797:1804	host immunity	1792:1804	host immunity	1792:1804	In addition, CNM as a promising AP may markedly enhance host immunity and therapeutic effects by oral administration.
36153996	8	30	theme	instrumental	1533:1544	arg1	link					1546:1549	an instrumental link	1530:1549	an instrumental link between the dosage and toxicity risk	1530:1586	In summary, these findings uncover an instrumental link between the dosage and toxicity risk, suggesting that APs need to be comprehensively assessed for risk before application as safe and reliable food preservatives or therapeutic agents.
36153996	4	31	dep	health	714:719	arg1	treat					759:763	treat	759:763	to treat serotype E. coli O157:H7 infection impacts	756:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	7	32	theme	%	1367:1367	arg1	CNM					1369:1371	oral 0.1% CNM	1359:1371	oral 0.1% CNM	1359:1371	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	4	33	theme	E.	774:775	arg1	O157					782:785	serotype E. coli O157	765:785	serotype E. coli O157:H7 infection impacts	765:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	3	34	theme	Chitosan	476:483	arg1	J25					508:510	Chitosan nanoparticles-microcin J25	476:510	Chitosan nanoparticles-microcin J25 (CNM)	476:516	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	34	theme	Chitosan	476:483	arg1	agent					632:636	a novel antimicrobial agent	610:636	a novel antimicrobial agent against bacterial infections	610:665	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	34	theme	Chitosan	476:483	arg1	class					521:525	a class	519:525	a class of APs engineered by bioactive peptides and chitosan nanoparticles	519:592	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	34	theme	Chitosan	476:483	arg1	CNM					513:515	CNM	513:515	CNM	513:515	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	0	35	theme	Polymeric	0:8	arg1	Nanohybrids					10:20	Polymeric Nanohybrids	0:20	Polymeric Nanohybrids	0:20	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	1	36	theme	promising	292:300	arg1	antimicrobials					311:324	promising clinical antimicrobials	292:324	promising clinical antimicrobials with rapidly increasing antibiotic resistance	292:370	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	1	36	theme	promising	292:300	arg1	attention					279:287	attention	279:287	attention	279:287	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	8	37	dep	dosage	1563:1568	arg1	risk					1583:1586	risk	1583:1586	risk	1583:1586	In summary, these findings uncover an instrumental link between the dosage and toxicity risk, suggesting that APs need to be comprehensively assessed for risk before application as safe and reliable food preservatives or therapeutic agents.
36153996	8	37	dep	dosage	1563:1568	arg1	the					1559:1561	the	1559:1561	the	1559:1561	In summary, these findings uncover an instrumental link between the dosage and toxicity risk, suggesting that APs need to be comprehensively assessed for risk before application as safe and reliable food preservatives or therapeutic agents.
36153996	7	38	theme	oral	1359:1362	arg1	CNM					1369:1371	oral 0.1% CNM	1359:1371	oral 0.1% CNM	1359:1371	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	4	39	theme	O157	782:785	arg1	impacts					800:806	serotype E. coli O157:H7 infection impacts	765:806	serotype E. coli O157:H7 infection impacts	765:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	9	40	theme	oral	1833:1836	arg1	administration					1838:1851	oral administration	1833:1851	oral administration	1833:1851	In addition, CNM as a promising AP may markedly enhance host immunity and therapeutic effects by oral administration.
36153996	2	41	theme	public	461:466	arg1	health					468:473	public health	461:473	public health	461:473	Infections by zoonotic enterohemorrhagic Escherichia coli are a severe global threat to public health.
36153996	0	42	theme	Chitosan	36:43	arg1	Nanoparticles					45:57	Chitosan Nanoparticles	36:57	Chitosan Nanoparticles	36:57	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	7	43	theme	Anti-infective	1324:1337	arg1	results					1339:1345	Anti-infective results	1324:1345	Anti-infective results	1324:1345	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	3	44	theme	APs	530:532	arg1	J25					508:510	Chitosan nanoparticles-microcin J25	476:510	Chitosan nanoparticles-microcin J25 (CNM)	476:516	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	44	theme	APs	530:532	arg1	class					521:525	a class	519:525	a class of APs engineered by bioactive peptides and chitosan nanoparticles	519:592	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	5	45	from	modulation	1136:1145	arg1	metabolites					1194:1204	metabolites	1194:1204	metabolites	1194:1204	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	45	from	modulation	1136:1145	arg1	compositions					1178:1189	gut microbiota compositions	1163:1189	gut microbiota compositions	1163:1189	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	46	theme	gut	1163:1165	arg1	compositions					1178:1189	gut microbiota compositions	1163:1189	gut microbiota compositions	1163:1189	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	0	47	dep	Engineered	22:31	arg1	Infection					205:213	H7 Infection	202:213	H7 Infection by Immune Regulation	202:234	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	8	48	theme	safe	1676:1679	arg1	preservatives					1699:1711	food preservatives	1694:1711	food preservatives	1694:1711	In summary, these findings uncover an instrumental link between the dosage and toxicity risk, suggesting that APs need to be comprehensively assessed for risk before application as safe and reliable food preservatives or therapeutic agents.
36153996	0	49	theme	H7	202:203	arg1	Infection					205:213	H7 Infection	202:213	H7 Infection by Immune Regulation	202:234	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	8	50	theme	reliable	1685:1692	arg1	preservatives					1699:1711	food preservatives	1694:1711	food preservatives	1694:1711	In summary, these findings uncover an instrumental link between the dosage and toxicity risk, suggesting that APs need to be comprehensively assessed for risk before application as safe and reliable food preservatives or therapeutic agents.
36153996	5	51	from	changes	1152:1158	arg1	metabolites					1194:1204	metabolites	1194:1204	metabolites	1194:1204	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	51	from	changes	1152:1158	arg1	compositions					1178:1189	gut microbiota compositions	1163:1189	gut microbiota compositions	1163:1189	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	4	52	from	health	714:719	arg1	assessment					686:695	the risk assessment	677:695	the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts	677:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	7	53	theme	Th1/Th2	1478:1484	arg1	balance					1486:1492	the Th1/Th2 balance	1474:1492	the Th1/Th2 balance	1474:1492	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	3	54	theme	chitosan	571:578	arg1	nanoparticles					580:592	chitosan nanoparticles	571:592	chitosan nanoparticles	571:592	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	8	55	theme	therapeutic	1716:1726	arg1	agents					1728:1733	therapeutic agents	1716:1733	therapeutic agents	1716:1733	In summary, these findings uncover an instrumental link between the dosage and toxicity risk, suggesting that APs need to be comprehensively assessed for risk before application as safe and reliable food preservatives or therapeutic agents.
36153996	1	56	with	antimicrobials	311:324	arg1	resistance					361:370	rapidly increasing antibiotic resistance	331:370	rapidly increasing antibiotic resistance	331:370	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	7	57	theme	immune	1442:1447	arg1	functions					1449:1457	immune functions	1442:1457	immune functions	1442:1457	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	1	58	theme	antibiotic	350:359	arg1	resistance					361:370	rapidly increasing antibiotic resistance	331:370	rapidly increasing antibiotic resistance	331:370	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	5	59	from	improvement	1058:1068	arg1	health					1077:1082	gut health	1073:1082	gut health	1073:1082	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	60	theme	oral	885:888	arg1	administration					890:903	oral administration	885:903	oral administration of low levels of CNM	885:924	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	0	61	from	Antimicrobials	95:108	arg1	Biopreservatives					118:133	Biopreservatives	118:133	Biopreservatives	118:133	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	62	theme	Anti-Foodborne	156:169	arg1	Antimicrobials					95:108	Novel Antimicrobials	89:108	Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157	89:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	62	theme	Anti-Foodborne	156:169	arg1	O157					197:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	5	63	theme	mouse	857:861	arg1	models					863:868	mouse models	857:868	mouse models	857:868	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	7	64	theme	O157	1411:1414	arg1	infection					1419:1427	E. coli O157:H7 infection	1403:1427	E. coli O157:H7 infection	1403:1427	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	4	65	theme	risk	681:684	arg1	assessment					686:695	the risk assessment	677:695	the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts	677:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	0	66	theme	Escherichia	180:190	arg1	Antimicrobials					95:108	Novel Antimicrobials	89:108	Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157	89:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	0	66	theme	Escherichia	180:190	arg1	O157					197:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Anti-Foodborne Pathogen Escherichia coli O157	156:200	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	3	67	theme	novel	612:616	arg1	agent					632:636	a novel antimicrobial agent	610:636	a novel antimicrobial agent against bacterial infections	610:665	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	67	theme	novel	612:616	arg1	J25					508:510	Chitosan nanoparticles-microcin J25	476:510	Chitosan nanoparticles-microcin J25 (CNM)	476:516	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	4	68	dep	O157	782:785	arg1	H7					787:788	H7	787:788	serotype E. coli O157:H7 infection impacts	765:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	7	69	theme	E.	1403:1404	arg1	O157					1411:1414	E. coli O157	1403:1414	E. coli O157:H7 infection	1403:1427	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	4	70	theme	immune	738:743	arg1	modulation					745:754	its potential immune modulation	724:754	its potential immune modulation	724:754	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	0	71	dep	Escherichia	180:190	arg1	coli					192:195	coli	192:195	coli	192:195	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	6	72	theme	high	1216:1219	arg1	concentrations					1221:1234	high concentrations	1216:1234	high concentrations of CNM	1216:1241	However, high concentrations of CNM induced serious adverse effects, negatively improving intestinal health targets.
36153996	5	73	theme	gut	1073:1075	arg1	health					1077:1082	gut health	1073:1082	gut health	1073:1082	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	3	74	theme	bacterial	646:654	arg1	infections					656:665	bacterial infections	646:665	bacterial infections	646:665	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	9	75	theme	promising	1758:1766	arg1	AP					1768:1769	a promising AP	1756:1769	a promising AP	1756:1769	In addition, CNM as a promising AP may markedly enhance host immunity and therapeutic effects by oral administration.
36153996	5	76	theme	levels	912:917	arg1	administration					890:903	oral administration	885:903	oral administration of low levels of CNM	885:924	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	77	theme	beneficial	975:984	arg1	effects					986:992	beneficial effects	975:992	beneficial effects on the lifespan or clinical signs	975:1026	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	78	theme	intestinal	1109:1118	arg1	barrier					1120:1126	the intestinal barrier	1105:1126	the intestinal barrier	1105:1126	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	7	79	theme	0.1	1364:1366	arg1	%					1367:1367	%	1367:1367	%	1367:1367	Anti-infective results proved that oral 0.1% CNM enhances host defense against E. coli O157:H7 infection by improving immune functions and modulating the Th1/Th2 balance.
36153996	4	80	theme	infection	790:798	arg1	impacts					800:806	serotype E. coli O157:H7 infection impacts	765:806	serotype E. coli O157:H7 infection impacts	765:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	2	81	theme	global	444:449	arg1	threat					451:456	a severe global threat	435:456	a severe global threat to public health	435:473	Infections by zoonotic enterohemorrhagic Escherichia coli are a severe global threat to public health.
36153996	2	81	theme	global	444:449	arg1	Infections					373:382	Infections	373:382	Infections by zoonotic enterohemorrhagic Escherichia coli	373:429	Infections by zoonotic enterohemorrhagic Escherichia coli are a severe global threat to public health.
36153996	5	82	from	barrier	1120:1126	arg1	metabolites					1194:1204	metabolites	1194:1204	metabolites	1194:1204	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	82	from	barrier	1120:1126	arg1	compositions					1178:1189	gut microbiota compositions	1163:1189	gut microbiota compositions	1163:1189	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	5	83	theme	body	954:957	arg1	weight					959:964	the body weight	950:964	the body weight	950:964	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	9	84	theme	therapeutic	1810:1820	arg1	effects					1822:1828	therapeutic effects	1810:1828	therapeutic effects	1810:1828	In addition, CNM as a promising AP may markedly enhance host immunity and therapeutic effects by oral administration.
36153996	4	85	theme	serotype	765:772	arg1	O157					782:785	serotype E. coli O157	765:785	serotype E. coli O157:H7 infection impacts	765:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	3	86	theme	nanoparticles-microcin	485:506	arg1	J25					508:510	Chitosan nanoparticles-microcin J25	476:510	Chitosan nanoparticles-microcin J25 (CNM)	476:516	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	86	theme	nanoparticles-microcin	485:506	arg1	agent					632:636	a novel antimicrobial agent	610:636	a novel antimicrobial agent against bacterial infections	610:665	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	86	theme	nanoparticles-microcin	485:506	arg1	class					521:525	a class	519:525	a class of APs engineered by bioactive peptides and chitosan nanoparticles	519:592	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	3	86	theme	nanoparticles-microcin	485:506	arg1	CNM					513:515	CNM	513:515	CNM	513:515	Chitosan nanoparticles-microcin J25 (CNM), a class of APs engineered by bioactive peptides and chitosan nanoparticles, can be used as a novel antimicrobial agent against bacterial infections.
36153996	1	87	theme	clinical	302:309	arg1	antimicrobials					311:324	promising clinical antimicrobials	292:324	promising clinical antimicrobials with rapidly increasing antibiotic resistance	292:370	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	1	87	theme	clinical	302:309	arg1	attention					279:287	attention	279:287	attention	279:287	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	4	88	theme	coli	777:780	arg1	O157					782:785	serotype E. coli O157	765:785	serotype E. coli O157:H7 infection impacts	765:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	0	89	theme	Antimicrobial	63:75	arg1	Peptides					77:84	Antimicrobial Peptides	63:84	Antimicrobial Peptides	63:84	Polymeric Nanohybrids Engineered by Chitosan Nanoparticles and Antimicrobial Peptides as Novel Antimicrobials in Food Biopreservatives: Risk Assessment and Anti-Foodborne Pathogen Escherichia coli O157:H7 Infection by Immune Regulation.
36153996	1	90	theme	increasing	339:348	arg1	resistance					361:370	rapidly increasing antibiotic resistance	331:370	rapidly increasing antibiotic resistance	331:370	Polymeric nanomaterials (APs) are gaining attention as promising clinical antimicrobials with rapidly increasing antibiotic resistance.
36153996	5	91	theme	microbiota	1167:1176	arg1	compositions					1178:1189	gut microbiota compositions	1163:1189	gut microbiota compositions	1163:1189	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	4	92	from	modulation	745:754	arg1	assessment					686:695	the risk assessment	677:695	the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts	677:806	However, the risk assessment of CNM on animal health or its potential immune modulation to treat serotype E. coli O157:H7 infection impacts are not well understood.
36153996	5	93	theme	clinical	1013:1020	arg1	signs					1022:1026	clinical signs	1013:1026	clinical signs	1013:1026	Herein, our findings in mouse models uncovered that oral administration of low levels of CNM significantly increased the body weight and made beneficial effects on the lifespan or clinical signs, accompanied by a significant improvement in gut health, including enhancing the intestinal barrier, immune modulation, and changes in gut microbiota compositions or metabolites.
36153996	8	94	theme	food	1694:1697	arg1	preservatives					1699:1711	food preservatives	1694:1711	food preservatives	1694:1711	In summary, these findings uncover an instrumental link between the dosage and toxicity risk, suggesting that APs need to be comprehensively assessed for risk before application as safe and reliable food preservatives or therapeutic agents.
37083594	13	0	theme	genus	1839:1843	arg1	Streptomyces					1845:1856	the genus Streptomyces	1835:1856	the genus Streptomyces	1835:1856	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	2	1	theme	Gram-stain-positive	105:123	arg1	actinobacterium					125:139	A Gram-stain-positive actinobacterium	103:139	A Gram-stain-positive actinobacterium	103:139	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	6	2	theme	distance	845:852	arg1	values					759:764	the values	755:764	the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives	755:934	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	5	3	theme	Streptomyces	687:698	arg1	65594T					717:722	Streptomyces endophyticus YIM 65594T	687:722	Streptomyces endophyticus YIM 65594T (similarity 96.80 %)	687:743	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	5	3	theme	Streptomyces	687:698	arg1	%					742:742	similarity 96.80 %	725:742	similarity 96.80 %	725:742	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	11	4	theme	strain	1570:1575	arg1	GXMU-J5T					1577:1584	strain GXMU-J5T	1570:1584	strain GXMU-J5T	1570:1584	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	13	5	theme	novel	1818:1822	arg1	species					1824:1830	a novel species	1816:1830	a novel species	1816:1830	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	2	6	theme	strain	153:158	arg1	GXMU-J5T					160:167	strain GXMU-J5T	153:167	strain GXMU-J5T	153:167	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	6	7	theme	nucleotide	808:817	arg1	identity					819:826	average nucleotide identity	800:826	average nucleotide identity	800:826	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	10	8	dep	iso-C15 	1469:1476	arg1	 0					1521:1522	 0	1521:1522	 0	1521:1522	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	10	8	dep	iso-C15 	1469:1476	arg1	 0					1495:1496	 0	1495:1496	 0	1495:1496	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	10	8	dep	iso-C15 	1469:1476	arg1	 0					1478:1479	 0	1478:1479	 0	1478:1479	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	10	8	dep	iso-C15 	1469:1476	arg1	 0					1541:1542	 0	1541:1542	 0	1541:1542	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	10	8	dep	iso-C15 	1469:1476	arg1	anteiso-C17 					1528:1539	anteiso-C17 	1528:1539	anteiso-C17 	1528:1539	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	15	9	theme	type	1929:1932	arg1	strain					1934:1939	The type strain	1925:1939	The type strain	1925:1939	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	15	9	theme	type	1929:1932	arg1	GXMU-J5T					1951:1958	strain GXMU-J5T	1944:1958	strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T)	1944:1986	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	13	10	dep	data	1762:1765	arg1	the					1718:1720	the	1718:1720	the	1718:1720	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	13	10	dep	data	1762:1765	arg1	basis					1722:1726	basis	1722:1726	basis	1722:1726	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	12	11	theme	Genomic	1630:1636	arg1	analysis					1638:1645	Genomic analysis	1630:1645	Genomic analysis	1630:1645	Genomic analysis indicated that strain GXMU-J5T had the potential to degrade chitin.
37083594	13	12	theme	strain	1789:1794	arg1	GXMU-J5T					1796:1803	strain GXMU-J5T	1789:1803	strain GXMU-J5T	1789:1803	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	3	13	from	classification	361:374	arg1	Streptomyces					389:400	the genus Streptomyces	379:400	the genus Streptomyces	379:400	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	5	14	theme	sequences	567:575	arg1	analysis					541:548	Phylogenetic analysis	528:548	Phylogenetic analysis of 16S rRNA gene sequences	528:575	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	3	15	theme	chemotaxonomic	288:301	arg1	consistent					341:350	consistent	341:350	consistent	341:350	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	3	15	theme	chemotaxonomic	288:301	arg1	characteristics					320:334	The morphological, chemotaxonomic and phylogenetic characteristics	269:334	The morphological, chemotaxonomic and phylogenetic characteristics	269:334	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	4	16	theme	abundant	472:479	arg1	hyphae					488:493	abundant aerial hyphae	472:493	abundant aerial hyphae that differentiated into spores	472:525	The organism formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores.
37083594	5	17	theme	YIM	713:715	arg1	65594T					717:722	Streptomyces endophyticus YIM 65594T	687:722	Streptomyces endophyticus YIM 65594T (similarity 96.80 %)	687:743	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	5	17	theme	YIM	713:715	arg1	%					742:742	similarity 96.80 %	725:742	similarity 96.80 %	725:742	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	2	18	theme	soil	212:215	arg1	sample					190:195	a sample	188:195	a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China	188:266	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	6	19	theme	distinct	968:975	arg1	species					977:983	a distinct species	966:983	a distinct species	966:983	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	3	20	theme	morphological	273:285	arg1	consistent					341:350	consistent	341:350	consistent	341:350	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	3	20	theme	morphological	273:285	arg1	characteristics					320:334	The morphological, chemotaxonomic and phylogenetic characteristics	269:334	The morphological, chemotaxonomic and phylogenetic characteristics	269:334	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	7	21	theme	major	1043:1047	arg1	xylose					1078:1083	xylose	1078:1083	xylose	1078:1083	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	7	21	theme	major	1043:1047	arg1	hydrolysates					1060:1071	the major whole-cell hydrolysates	1039:1071	the major whole-cell hydrolysates	1039:1071	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	4	22	theme	substrate	447:455	arg1	mycelium					457:464	an extensively branched substrate mycelium	423:464	an extensively branched substrate mycelium	423:464	The organism formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores.
37083594	2	23	dep	China	262:266	arg1	PR					259:260	Tieshangang Saltern, Beihai, PR China	230:266	PR	259:260	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	2	24	theme	Saltern	242:248	arg1	Beihai					251:256	Beihai	251:256	Beihai	251:256	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	2	24	theme	Saltern	242:248	arg1	China					262:266	Tieshangang Saltern, Beihai, PR China	230:266	China	262:266	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	5	25	theme	rRNA	557:560	arg1	sequences					567:575	16S rRNA gene sequences	553:575	16S rRNA gene sequences	553:575	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	3	26	theme	phylogenetic	307:318	arg1	consistent					341:350	consistent	341:350	consistent	341:350	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	3	26	theme	phylogenetic	307:318	arg1	characteristics					320:334	The morphological, chemotaxonomic and phylogenetic characteristics	269:334	The morphological, chemotaxonomic and phylogenetic characteristics	269:334	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	15	27	theme	1K08064T=JCM	1967:1978	arg1	GXMU-J5T					1951:1958	strain GXMU-J5T	1944:1958	strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T)	1944:1986	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	15	27	theme	1K08064T=JCM	1967:1978	arg1	35629T					1980:1985	=MCCC 1K08064T=JCM 35629T	1961:1985	=MCCC 1K08064T=JCM 35629T	1961:1985	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	7	28	theme	ll-diaminopimelic	1012:1028	arg1	acid					1030:1033	ll-diaminopimelic acid	1012:1033	ll-diaminopimelic acid	1012:1033	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	13	29	theme	genotypic	1737:1745	arg1	data					1762:1765	these genotypic and phenotypic data	1731:1765	these genotypic and phenotypic data	1731:1765	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	11	30	theme	genome	1555:1560	arg1	size					1562:1565	The whole genome size	1545:1565	The whole genome size of strain GXMU-J5T	1545:1584	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	11	30	theme	genome	1555:1560	arg1	Mbp					1595:1597	6.79 Mbp	1590:1597	6.79 Mbp	1590:1597	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	9	31	theme	unknown	1385:1391	arg1	structure					1393:1401	unknown structure	1385:1401	unknown structure containing glucosamine	1385:1424	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	15	32	theme	strain	1944:1949	arg1	strain					1934:1939	The type strain	1925:1939	The type strain	1925:1939	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	15	32	theme	strain	1944:1949	arg1	GXMU-J5T					1951:1958	strain GXMU-J5T	1944:1958	strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T)	1944:1986	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	15	32	theme	strain	1944:1949	arg1	35629T					1980:1985	=MCCC 1K08064T=JCM 35629T	1961:1985	=MCCC 1K08064T=JCM 35629T	1961:1985	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	13	33	theme	name	1873:1876	arg1	sp					1903:1904	the name Streptomyces beihaiensis sp	1869:1904	the name Streptomyces beihaiensis sp	1869:1904	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	13	34	theme	beihaiensis	1891:1901	arg1	sp					1903:1904	the name Streptomyces beihaiensis sp	1869:1904	the name Streptomyces beihaiensis sp	1869:1904	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	0	35	theme	Streptomyces	0:11	arg1	sp					25:26	Streptomyces beihaiensis sp	0:26	Streptomyces beihaiensis sp.	0:27	Streptomyces beihaiensis sp.
37083594	8	36	theme	strain	1132:1137	arg1	GXMU-J5T					1139:1146	strain GXMU-J5T	1132:1146	strain GXMU-J5T	1132:1146	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	1	37	theme	pond	92:95	arg1	soil					97:100	shrimp pond soil	85:100	shrimp pond soil	85:100	nov., a chitin-degrading actinobacterium, isolated from shrimp pond soil.
37083594	6	38	theme	identity	819:826	arg1	values					759:764	the values	755:764	the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives	755:934	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	9	39	contain	containing	1403:1412	arg2	glucosamine					1414:1424	glucosamine	1414:1424	glucosamine	1414:1424	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	9	39	contain	containing	1403:1412	arg1	structure					1393:1401	unknown structure	1385:1401	unknown structure containing glucosamine	1385:1424	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	4	40	with	mycelium	457:464	arg1	hyphae					488:493	abundant aerial hyphae	472:493	abundant aerial hyphae that differentiated into spores	472:525	The organism formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores.
37083594	5	41	theme	16S	553:555	arg1	sequences					567:575	16S rRNA gene sequences	553:575	16S rRNA gene sequences	553:575	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	6	42	theme	strain	894:899	arg1	GXMU-J5T					901:908	strain GXMU-J5T	894:908	strain GXMU-J5T	894:908	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	2	43	attach	isolated	174:181	arg1	sample					190:195	a sample	188:195	a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China	188:266	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	2	43	attach	isolated	174:181	arg2	actinobacterium					125:139	A Gram-stain-positive actinobacterium	103:139	A Gram-stain-positive actinobacterium	103:139	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	10	44	theme	predominant	1431:1441	arg1	acids					1458:1462	The predominant cellular fatty acids	1427:1462	The predominant cellular fatty acids	1427:1462	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	10	44	theme	predominant	1431:1441	arg1	iso-C15 					1469:1476	iso-C15 	1469:1476	iso-C15 	1469:1476	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	6	45	theme	analysis	877:884	arg1	hybridization					785:797	digital DNA-DNA hybridization	769:797	digital DNA-DNA hybridization	769:797	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	6	45	theme	analysis	877:884	arg1	identity					819:826	average nucleotide identity	800:826	average nucleotide identity	800:826	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	6	45	theme	analysis	877:884	arg1	distance					845:852	evolutionary distance	832:852	evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives	832:934	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	2	46	theme	shrimp	200:205	arg1	soil					212:215	shrimp pond soil	200:215	shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China	200:266	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	11	47	theme	G+C	1617:1619	arg1	content					1621:1627	a 71.39 mol% G+C content	1604:1627	a 71.39 mol% G+C content	1604:1627	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	6	48	theme	multilocus	857:866	arg1	analysis					877:884	multilocus sequence analysis	857:884	multilocus sequence analysis	857:884	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	6	49	theme	DNA-DNA	777:783	arg1	hybridization					785:797	digital DNA-DNA hybridization	769:797	digital DNA-DNA hybridization	769:797	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	5	50	theme	Phylogenetic	528:539	arg1	analysis					541:548	Phylogenetic analysis	528:548	Phylogenetic analysis of 16S rRNA gene sequences	528:575	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	1	51	theme	chitin-degrading	37:52	arg1	actinobacterium					54:68	a chitin-degrading actinobacterium	35:68	a chitin-degrading actinobacterium	35:68	nov., a chitin-degrading actinobacterium, isolated from shrimp pond soil.
37083594	1	51	theme	chitin-degrading	37:52	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a chitin-degrading actinobacterium, isolated from shrimp pond soil.
37083594	10	52	theme	fatty	1452:1456	arg1	acids					1458:1462	The predominant cellular fatty acids	1427:1462	The predominant cellular fatty acids	1427:1462	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	10	52	theme	fatty	1452:1456	arg1	iso-C15 					1469:1476	iso-C15 	1469:1476	iso-C15 	1469:1476	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	6	53	theme	average	800:806	arg1	identity					819:826	average nucleotide identity	800:826	average nucleotide identity	800:826	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	6	54	theme	evolutionary	832:843	arg1	distance					845:852	evolutionary distance	832:852	evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives	832:934	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	6	55	theme	digital	769:775	arg1	hybridization					785:797	digital DNA-DNA hybridization	769:797	digital DNA-DNA hybridization	769:797	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	9	56	theme	polar	1202:1206	arg1	lipids					1208:1213	The polar lipids	1198:1213	The polar lipids	1198:1213	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	6	57	theme	closest	918:924	arg1	relatives					926:934	its closest relatives	914:934	its closest relatives	914:934	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	5	58	theme	similarity	663:672	arg1	41681T					655:660	Streptomyces kunmingensis DSM 41681T	625:660	Streptomyces kunmingensis DSM 41681T (similarity 97.74 %)	625:681	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	5	58	theme	similarity	663:672	arg1	%					680:680	similarity 97.74 %	663:680	similarity 97.74 %	663:680	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	11	59	theme	71.39 mol	1606:1614	arg1	content					1621:1627	a 71.39 mol% G+C content	1604:1627	a 71.39 mol% G+C content	1604:1627	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	5	60	theme	similarity	725:734	arg1	65594T					717:722	Streptomyces endophyticus YIM 65594T	687:722	Streptomyces endophyticus YIM 65594T (similarity 96.80 %)	687:743	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	5	60	theme	similarity	725:734	arg1	%					742:742	similarity 96.80 %	725:742	similarity 96.80 %	725:742	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	6	61	theme	hybridization	785:797	arg1	values					759:764	the values	755:764	the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives	755:934	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	7	62	contain	contained	1002:1010	arg2	acid					1030:1033	ll-diaminopimelic acid	1012:1033	ll-diaminopimelic acid	1012:1033	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	7	62	contain	contained	1002:1010	arg1	GXMU-J5T					993:1000	Strain GXMU-J5T	986:1000	Strain GXMU-J5T	986:1000	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	2	63	theme	Tieshangang	230:240	arg1	Beihai					251:256	Beihai	251:256	Beihai	251:256	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	2	63	theme	Tieshangang	230:240	arg1	China					262:266	Tieshangang Saltern, Beihai, PR China	230:266	China	262:266	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	4	64	theme	aerial	481:486	arg1	hyphae					488:493	abundant aerial hyphae	472:493	abundant aerial hyphae that differentiated into spores	472:525	The organism formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores.
37083594	4	65	theme	branched	438:445	arg1	mycelium					457:464	an extensively branched substrate mycelium	423:464	an extensively branched substrate mycelium	423:464	The organism formed an extensively branched substrate mycelium, with abundant aerial hyphae that differentiated into spores.
37083594	5	66	theme	strain	589:594	arg1	GXMU-J5T					596:603	strain GXMU-J5T	589:603	strain GXMU-J5T	589:603	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	9	67	theme	phosphatidylinositol	1332:1351	arg1	mannosides					1353:1362	phosphatidylinositol mannosides	1332:1362	phosphatidylinositol mannosides	1332:1362	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	7	68	theme	whole-cell	1049:1058	arg1	xylose					1078:1083	xylose	1078:1083	xylose	1078:1083	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	7	68	theme	whole-cell	1049:1058	arg1	hydrolysates					1060:1071	the major whole-cell hydrolysates	1039:1071	the major whole-cell hydrolysates	1039:1071	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	3	69	with	consistent	341:350	arg1	classification					361:374	its classification	357:374	its classification in the genus Streptomyces	357:400	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	11	70	theme	GXMU-J5T	1577:1584	arg1	size					1562:1565	The whole genome size	1545:1565	The whole genome size of strain GXMU-J5T	1545:1584	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	11	70	theme	GXMU-J5T	1577:1584	arg1	Mbp					1595:1597	6.79 Mbp	1590:1597	6.79 Mbp	1590:1597	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	5	71	theme	gene	562:565	arg1	sequences					567:575	16S rRNA gene sequences	553:575	16S rRNA gene sequences	553:575	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	11	72	theme	whole	1549:1553	arg1	size					1562:1565	The whole genome size	1545:1565	The whole genome size of strain GXMU-J5T	1545:1584	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	11	72	theme	whole	1549:1553	arg1	Mbp					1595:1597	6.79 Mbp	1590:1597	6.79 Mbp	1590:1597	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	8	73	theme	predominant	1104:1114	arg1	MK-9					1188:1191	MK-9	1188:1191	MK-9	1188:1191	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	8	73	theme	predominant	1104:1114	arg1	MK-9					1175:1178	MK-9	1175:1178	MK-9	1175:1178	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	8	73	theme	predominant	1104:1114	arg1	MK-9					1165:1168	MK-9	1165:1168	MK-9	1165:1168	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	8	73	theme	predominant	1104:1114	arg1	menaquinones					1116:1127	The predominant menaquinones	1100:1127	The predominant menaquinones of strain GXMU-J5T	1100:1146	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	5	74	theme	Streptomyces	625:636	arg1	41681T					655:660	Streptomyces kunmingensis DSM 41681T	625:660	Streptomyces kunmingensis DSM 41681T (similarity 97.74 %)	625:681	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	5	74	theme	Streptomyces	625:636	arg1	%					680:680	similarity 97.74 %	663:680	similarity 97.74 %	663:680	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	15	75	theme	=MCCC	1961:1965	arg1	GXMU-J5T					1951:1958	strain GXMU-J5T	1944:1958	strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T)	1944:1986	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	15	75	theme	=MCCC	1961:1965	arg1	35629T					1980:1985	=MCCC 1K08064T=JCM 35629T	1961:1985	=MCCC 1K08064T=JCM 35629T	1961:1985	The type strain is strain GXMU-J5T (=MCCC 1K08064T=JCM 35629T).
37083594	9	76	theme	structure	1393:1401	arg1	phosphatidylmethylethanolamine					1252:1281	phosphatidylmethylethanolamine	1252:1281	phosphatidylmethylethanolamine	1252:1281	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	9	76	theme	structure	1393:1401	arg1	mannosides					1353:1362	phosphatidylinositol mannosides	1332:1362	phosphatidylinositol mannosides	1332:1362	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	9	76	theme	structure	1393:1401	arg1	phosphatidylethanolamine					1284:1307	phosphatidylethanolamine	1284:1307	phosphatidylethanolamine	1284:1307	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	9	76	theme	structure	1393:1401	arg1	phospholipids					1368:1380	phospholipids	1368:1380	phospholipids of unknown structure containing glucosamine	1368:1424	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	9	76	theme	structure	1393:1401	arg1	diphosphatidylglycerol					1228:1249	diphosphatidylglycerol	1228:1249	diphosphatidylglycerol	1228:1249	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	9	76	theme	structure	1393:1401	arg1	phosphatidylglycerol					1310:1329	phosphatidylglycerol	1310:1329	phosphatidylglycerol	1310:1329	The polar lipids consisted of diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides and phospholipids of unknown structure containing glucosamine.
37083594	5	77	theme	DSM	651:653	arg1	41681T					655:660	Streptomyces kunmingensis DSM 41681T	625:660	Streptomyces kunmingensis DSM 41681T (similarity 97.74 %)	625:681	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	5	77	theme	DSM	651:653	arg1	%					680:680	similarity 97.74 %	663:680	similarity 97.74 %	663:680	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	13	78	theme	phenotypic	1751:1760	arg1	data					1762:1765	these genotypic and phenotypic data	1731:1765	these genotypic and phenotypic data	1731:1765	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	13	79	theme	Streptomyces	1878:1889	arg1	sp					1903:1904	the name Streptomyces beihaiensis sp	1869:1904	the name Streptomyces beihaiensis sp	1869:1904	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	0	80	theme	beihaiensis	13:23	arg1	sp					25:26	Streptomyces beihaiensis sp	0:26	Streptomyces beihaiensis sp.	0:27	Streptomyces beihaiensis sp.
37083594	8	81	theme	GXMU-J5T	1139:1146	arg1	MK-9					1188:1191	MK-9	1188:1191	MK-9	1188:1191	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	8	81	theme	GXMU-J5T	1139:1146	arg1	MK-9					1175:1178	MK-9	1175:1178	MK-9	1175:1178	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	8	81	theme	GXMU-J5T	1139:1146	arg1	MK-9					1165:1168	MK-9	1165:1168	MK-9	1165:1168	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	8	81	theme	GXMU-J5T	1139:1146	arg1	menaquinones					1116:1127	The predominant menaquinones	1100:1127	The predominant menaquinones of strain GXMU-J5T	1100:1146	The predominant menaquinones of strain GXMU-J5T were revealed as MK-9(H4), MK-9(H6) and MK-9(H8).
37083594	7	82	theme	Strain	986:991	arg1	GXMU-J5T					993:1000	Strain GXMU-J5T	986:1000	Strain GXMU-J5T	986:1000	Strain GXMU-J5T contained ll-diaminopimelic acid and the major whole-cell hydrolysates were xylose and galactose.
37083594	1	83	theme	shrimp	85:90	arg1	soil					97:100	shrimp pond soil	85:100	shrimp pond soil	85:100	nov., a chitin-degrading actinobacterium, isolated from shrimp pond soil.
37083594	5	84	dep	Streptomyces	687:698	arg1	endophyticus					700:711	endophyticus	700:711	endophyticus	700:711	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	11	85	with	Mbp	1595:1597	arg1	content					1621:1627	a 71.39 mol% G+C content	1604:1627	a 71.39 mol% G+C content	1604:1627	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	13	86	theme	Streptomyces	1845:1856	arg1	species					1824:1830	a novel species	1816:1830	a novel species	1816:1830	On the basis of these genotypic and phenotypic data, it is supported that strain GXMU-J5T represents a novel species of the genus Streptomyces, for which the name Streptomyces beihaiensis sp.
37083594	12	87	theme	strain	1662:1667	arg1	GXMU-J5T					1669:1676	strain GXMU-J5T	1662:1676	strain GXMU-J5T	1662:1676	Genomic analysis indicated that strain GXMU-J5T had the potential to degrade chitin.
37083594	3	88	theme	genus	383:387	arg1	Streptomyces					389:400	the genus Streptomyces	379:400	the genus Streptomyces	379:400	The morphological, chemotaxonomic and phylogenetic characteristics were consistent with its classification in the genus Streptomyces.
37083594	10	89	theme	cellular	1443:1450	arg1	acids					1458:1462	The predominant cellular fatty acids	1427:1462	The predominant cellular fatty acids	1427:1462	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	10	89	theme	cellular	1443:1450	arg1	iso-C15 					1469:1476	iso-C15 	1469:1476	iso-C15 	1469:1476	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	2	90	theme	pond	207:210	arg1	soil					212:215	shrimp pond soil	200:215	shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China	200:266	A Gram-stain-positive actinobacterium, designated strain GXMU-J5T, was isolated from a sample of shrimp pond soil collected in Tieshangang Saltern, Beihai, PR China.
37083594	11	91	theme	%	1615:1615	arg1	content					1621:1627	a 71.39 mol% G+C content	1604:1627	a 71.39 mol% G+C content	1604:1627	The whole genome size of strain GXMU-J5T was 6.79 Mbp with a 71.39 mol% G+C content.
37083594	10	92	dep	 0	1495:1496	arg1	 0					1508:1509	 0	1508:1509	 0	1508:1509	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
37083594	6	93	theme	sequence	868:875	arg1	analysis					877:884	multilocus sequence analysis	857:884	multilocus sequence analysis	857:884	However, the values of digital DNA-DNA hybridization, average nucleotide identity and evolutionary distance of multilocus sequence analysis between strain GXMU-J5T and its closest relatives indicated that it represented a distinct species.
37083594	5	94	dep	Streptomyces	625:636	arg1	kunmingensis					638:649	kunmingensis	638:649	kunmingensis	638:649	Phylogenetic analysis of 16S rRNA gene sequences showed that strain GXMU-J5T was most related to Streptomyces kunmingensis DSM 41681T (similarity 97.74 %) and Streptomyces endophyticus YIM 65594T (similarity 96.80 %).
37083594	12	95	contain	had	1678:1680	arg1	GXMU-J5T					1669:1676	strain GXMU-J5T	1662:1676	strain GXMU-J5T	1662:1676	Genomic analysis indicated that strain GXMU-J5T had the potential to degrade chitin.
37083594	12	95	contain	had	1678:1680	arg2	potential					1686:1694	the potential to degrade chitin	1682:1712	the potential to degrade chitin	1682:1712	Genomic analysis indicated that strain GXMU-J5T had the potential to degrade chitin.
37279587	11	0	contain	had	1501:1503	arg2	incidence					1521:1529	similar disease incidence	1505:1529	similar disease incidence	1505:1529	Prior to the experiment, cows in both treatments had similar disease incidence.
37279587	11	0	contain	had	1501:1503	arg1	cows					1477:1480	cows	1477:1480	cows in both treatments	1477:1499	Prior to the experiment, cows in both treatments had similar disease incidence.
37279587	5	1	theme	treatments	977:986	arg1	onset					968:972	the onset	964:972	the onset of treatments	964:986	All cows were fed the same diet containing OG for at least one year before the onset of treatments.
37279587	2	2	theme	dairy	530:534	arg1	cows					536:539	mid-lactation dairy cows	516:539	mid-lactation dairy cows	516:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	16	3	theme	cows	2317:2320	arg1	diet					2293:2296	the diet	2289:2296	the diet of lactating dairy cows	2289:2320	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	3	4	dep	placebo	771:777	arg1	CTL					780:782	CTL	780:782	CTL	780:782	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	4	dep	placebo	771:777	arg1	56 g/d/cow					785:794	56 g/d/cow	785:794	56 g/d/cow	785:794	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	1	5	theme	immune	251:256	arg1	competency					258:267	immune competency	251:267	immune competency	251:267	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	14	6	dep	score	1764:1768	arg1	=					1788:1788	=	1788:1788	=	1788:1788	Compared with CTL, feeding OG maintained greater body condition score (2.83 vs. 2.92, P = 0.04).
37279587	4	7	theme	same	822:825	arg1	pen					838:840	the same free-stall pen	818:840	the same free-stall pen	818:840	Cows were housed in the same free-stall pen and individually fed 1×/d through Calan gates.
37279587	1	8	theme	additives	195:203	arg1	Supplementation					154:168	Supplementation	154:168	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG),	154:231	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	6	9	theme	milk	1015:1018	arg1	yield					1020:1024	milk yield	1015:1024	milk yield	1015:1024	Cows were milked 3×/d and milk yield was recorded at each milking.
37279587	0	10	theme	blood	116:120	arg1	cell					134:137	peripheral blood mononuclear cell	105:137	peripheral blood mononuclear cell proliferation	105:151	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.
37279587	16	11	from	diet	2293:2296	arg1	withdrawal					2273:2282	its withdrawal	2269:2282	its withdrawal from the diet of lactating dairy cows	2269:2320	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	1	12	theme	OmniGen	214:220	arg1	OG					228:229	OG	228:229	OG	228:229	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	1	12	theme	OmniGen	214:220	arg1	AF					224:225	OmniGen ® AF	214:225	OmniGen ® AF (OG)	214:230	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	2	13	theme	mononuclear	474:484	arg1	cells					486:490	peripheral blood mononuclear cells	457:490	peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	457:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	2	13	theme	mononuclear	474:484	arg1	PBMC					493:496	PBMC	493:496	PBMC	493:496	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	10	14	theme	concanavalin	1344:1355	arg1	ConA					1360:1363	ConA	1360:1363	ConA	1360:1363	The PBMC were cultured with concanavalin A (ConA) and lipopolysaccharides (LPS) for 72 h in vitro to determine proliferative responses.
37279587	10	14	theme	concanavalin	1344:1355	arg1	A					1357:1357	concanavalin A	1344:1357	concanavalin A (ConA)	1344:1364	The PBMC were cultured with concanavalin A (ConA) and lipopolysaccharides (LPS) for 72 h in vitro to determine proliferative responses.
37279587	0	15	theme	cell	134:137	arg1	proliferation					139:151	peripheral blood mononuclear cell proliferation	105:151	peripheral blood mononuclear cell proliferation	105:151	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.
37279587	15	16	theme	stimulation	2036:2046	arg1	index					2048:2052	stimulation index	2036:2052	stimulation index	2036:2052	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	16	17	theme	cows	2142:2145	arg1	diet					2120:2123	the diet	2116:2123	the diet of mid-lactation cows	2116:2145	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	11	18	theme	disease	1513:1519	arg1	incidence					1521:1529	similar disease incidence	1505:1529	similar disease incidence	1505:1529	Prior to the experiment, cows in both treatments had similar disease incidence.
37279587	3	19	with	dressed	732:738	arg1	56 g/d/cow					756:765	56 g/d/cow	756:765	56 g/d/cow	756:765	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	19	with	dressed	732:738	arg1	placebo					771:777	placebo	771:777	placebo (CTL, 56 g/d/cow)	771:795	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	19	with	dressed	732:738	arg1	OG					752:753	OG	752:753	OG (56 g/d/cow)	752:766	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	16	20	theme	proliferative	2155:2167	arg1	response					2169:2176	proliferative response	2155:2176	proliferative response of PBMC	2155:2184	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	15	21	theme	stimulation	1924:1934	arg1	LPS					1919:1921	LPS	1919:1921	LPS (stimulation index: 1.27 vs. 1.80, P = 0.05)	1919:1966	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	21	theme	stimulation	1924:1934	arg1	index					1936:1940	stimulation index	1924:1940	stimulation index	1924:1940	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	2	22	theme	OG	437:438	arg1	effect					415:420	the effect	411:420	the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	411:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	3	23	theme	treatments	687:696	arg1	treatments					687:696	the two dietary treatments	671:696	the two dietary treatments	671:696	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	23	theme	treatments	687:696	arg1	one					664:666	one	664:666	one	664:666	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	16	24	theme	lactating	2301:2309	arg1	cows					2317:2320	lactating dairy cows	2301:2320	lactating dairy cows	2301:2320	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	15	25	theme	greater	1871:1877	arg1	rate					1893:1896	a greater proliferative rate	1869:1896	a greater proliferative rate	1869:1896	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	5	26	theme	same	911:914	arg1	diet					916:919	the same diet	907:919	the same diet containing OG for at least one year	907:955	All cows were fed the same diet containing OG for at least one year before the onset of treatments.
37279587	3	27	theme	Holstein	554:561	arg1	n					569:569	n = 32	569:574	n = 32	569:574	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	27	theme	Holstein	554:561	arg1	cows					563:566	Multiparous Holstein cows	542:566	Multiparous Holstein cows (n = 32)	542:575	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	2	28	theme	withdrawing	425:435	arg1	OG					437:438	withdrawing OG	425:438	withdrawing OG from the diet	425:452	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	1	29	theme	immunomodulatory	173:188	arg1	additives					195:203	immunomodulatory feed additives	173:203	immunomodulatory feed additives	173:203	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	1	29	theme	immunomodulatory	173:188	arg1	AF					224:225	OmniGen ® AF	214:225	OmniGen ® AF (OG)	214:230	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	15	30	dep	index	1936:1940	arg1	1.80					1952:1955	1.80	1952:1955	1.80	1952:1955	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	30	dep	index	1936:1940	arg1	1.27					1943:1946	1.27	1943:1946	1.27	1943:1946	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	30	dep	index	1936:1940	arg1	P					1958:1958	P = 0.05	1958:1965	P = 0.05	1958:1965	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	2	31	theme	peripheral	457:466	arg1	cells					486:490	peripheral blood mononuclear cells	457:490	peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	457:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	2	31	theme	peripheral	457:466	arg1	PBMC					493:496	PBMC	493:496	PBMC	493:496	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	13	32	theme	Withdrawing	1594:1604	arg1	OG					1606:1607	Withdrawing OG	1594:1607	Withdrawing OG from the diet	1594:1621	Withdrawing OG from the diet did not affect (P ≥ 0.20) milk yield or composition, intake, or body weight.
37279587	0	33	theme	cow	92:94	arg1	diets					96:100	cow diets	92:100	cow diets	92:100	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.
37279587	13	34	theme	body	1687:1690	arg1	weight					1692:1697	body weight	1687:1697	body weight	1687:1697	Withdrawing OG from the diet did not affect (P ≥ 0.20) milk yield or composition, intake, or body weight.
37279587	14	35	theme	body	1749:1752	arg1	score					1764:1768	greater body condition score	1741:1768	greater body condition score (2.83 vs. 2.92, P = 0.04)	1741:1794	Compared with CTL, feeding OG maintained greater body condition score (2.83 vs. 2.92, P = 0.04).
37279587	15	36	attach	isolated	1839:1846	arg2	PBMC					1834:1837	PBMC	1834:1837	PBMC isolated from cows fed OG	1834:1863	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	36	attach	isolated	1839:1846	arg1	cows					1853:1856	cows	1853:1856	cows fed OG	1853:1863	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	1	37	attach	removed	350:356	arg2	OG					344:345	OG	344:345	OG	344:345	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	1	37	attach	removed	350:356	arg1	diet					367:370	the diet	363:370	the diet	363:370	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	15	38	theme	proliferative	1879:1891	arg1	rate					1893:1896	a greater proliferative rate	1869:1896	a greater proliferative rate	1869:1896	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	39	theme	=	1960:1960	arg1	P					1958:1958	P = 0.05	1958:1965	P = 0.05	1958:1965	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	16	40	theme	OG	2231:2232	arg1	role					2223:2226	the immunomodulatory role	2202:2226	the immunomodulatory role of OG	2202:2232	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	16	40	theme	OG	2231:2232	arg1	week					2258:2261	one week	2254:2261	one week after its withdrawal from the diet of lactating dairy cows	2254:2320	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	4	41	theme	free-stall	827:836	arg1	pen					838:840	the same free-stall pen	818:840	the same free-stall pen	818:840	Cows were housed in the same free-stall pen and individually fed 1×/d through Calan gates.
37279587	8	42	theme	Body	1145:1148	arg1	weight					1150:1155	Body weight	1145:1155	Body weight	1145:1155	Body weight and condition score were measured weekly.
37279587	15	43	dep	index	2048:2052	arg1	7.80					2064:2067	7.80	2064:2067	7.80	2064:2067	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	43	dep	index	2048:2052	arg1	5.24					2055:2058	5.24	2055:2058	5.24	2055:2058	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	43	dep	index	2048:2052	arg1	0.08					2074:2077	0.08	2074:2077	0.08	2074:2077	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	0	44	theme	Short	0:4	arg1	Communication					6:18	Short Communication	0:18	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.	0:152	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.
37279587	13	45	from	diet	1618:1621	arg1	OG					1606:1607	Withdrawing OG	1594:1607	Withdrawing OG from the diet	1594:1621	Withdrawing OG from the diet did not affect (P ≥ 0.20) milk yield or composition, intake, or body weight.
37279587	14	46	theme	feeding	1719:1725	arg1	OG					1727:1728	feeding OG	1719:1728	feeding OG	1719:1728	Compared with CTL, feeding OG maintained greater body condition score (2.83 vs. 2.92, P = 0.04).
37279587	9	47	theme	PBMC	1310:1313	arg1	isolation					1297:1305	the isolation	1293:1305	the isolation of PBMC	1293:1313	Blood samples were collected at -1, 1, 3, 5, and 7 wk relative to the onset of treatments for the isolation of PBMC.
37279587	3	48	theme	=	571:571	arg1	n					569:569	n = 32	569:574	n = 32	569:574	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	48	theme	=	571:571	arg1	cows					563:566	Multiparous Holstein cows	542:566	Multiparous Holstein cows (n = 32)	542:575	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	11	49	from	cows	1477:1480	arg1	treatments					1490:1499	both treatments	1485:1499	both treatments	1485:1499	Prior to the experiment, cows in both treatments had similar disease incidence.
37279587	1	50	theme	immune	296:301	arg1	benefits					303:310	immune benefits	296:310	immune benefits	296:310	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	7	51	dep	milkings	1103:1110	arg1	composition					1123:1133	composition	1123:1133	composition	1123:1133	Milk samples were collected from 3 consecutive milkings weekly and composition analyzed.
37279587	7	51	dep	milkings	1103:1110	arg1	weekly					1112:1117	weekly	1112:1117	weekly	1112:1117	Milk samples were collected from 3 consecutive milkings weekly and composition analyzed.
37279587	7	51	dep	milkings	1103:1110	arg1	milkings					1103:1110	3 consecutive milkings weekly and composition	1089:1133	3 consecutive milkings weekly and composition analyzed	1089:1142	Milk samples were collected from 3 consecutive milkings weekly and composition analyzed.
37279587	2	52	theme	cows	536:539	arg1	proliferation					499:511	peripheral blood mononuclear cells (PBMC) proliferation	457:511	peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	457:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	15	53	contain	have	1982:1985	arg2	proliferation					1995:2007	greater proliferation	1987:2007	greater proliferation	1987:2007	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	53	contain	have	1982:1985	arg1	PBMC					1834:1837	PBMC	1834:1837	PBMC isolated from cows fed OG	1834:1863	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	16	54	from	diet	2120:2123	arg1	OG					2108:2109	withdrawing OG	2096:2109	withdrawing OG from the diet of mid-lactation cows	2096:2145	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	9	55	theme	Blood	1199:1203	arg1	samples					1205:1211	Blood samples	1199:1211	Blood samples	1199:1211	Blood samples were collected at -1, 1, 3, 5, and 7 wk relative to the onset of treatments for the isolation of PBMC.
37279587	2	56	theme	mid-lactation	516:528	arg1	cows					536:539	mid-lactation dairy cows	516:539	mid-lactation dairy cows	516:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	16	57	theme	dairy	2311:2315	arg1	cows					2317:2320	lactating dairy cows	2301:2320	lactating dairy cows	2301:2320	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	1	58	theme	feed	190:193	arg1	additives					195:203	immunomodulatory feed additives	173:203	immunomodulatory feed additives	173:203	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	1	58	theme	feed	190:193	arg1	AF					224:225	OmniGen ® AF	214:225	OmniGen ® AF (OG)	214:230	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	3	59	from	days	612:615	arg1	d					635:635	153 ± 39 d	626:635	153 ± 39 d	626:635	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	59	from	days	612:615	arg1	milk					620:623	milk	620:623	milk (153 ± 39 d)	620:636	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	60	theme	±	630:630	arg1	d					635:635	153 ± 39 d	626:635	153 ± 39 d	626:635	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	60	theme	±	630:630	arg1	milk					620:623	milk	620:623	milk (153 ± 39 d)	620:636	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	0	61	theme	mononuclear	122:132	arg1	cell					134:137	peripheral blood mononuclear cell	105:137	peripheral blood mononuclear cell proliferation	105:151	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.
37279587	16	62	theme	mid-lactation	2128:2140	arg1	cows					2142:2145	mid-lactation cows	2128:2145	mid-lactation cows	2128:2145	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	2	63	theme	cells	486:490	arg1	proliferation					499:511	peripheral blood mononuclear cells (PBMC) proliferation	457:511	peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	457:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	15	64	contain	had	1865:1867	arg1	PBMC					1834:1837	PBMC	1834:1837	PBMC isolated from cows fed OG	1834:1863	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	15	64	contain	had	1865:1867	arg2	rate					1893:1896	a greater proliferative rate	1869:1896	a greater proliferative rate	1869:1896	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	1	65	theme	®	222:222	arg1	OG					228:229	OG	228:229	OG	228:229	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	1	65	theme	®	222:222	arg1	AF					224:225	OmniGen ® AF	214:225	OmniGen ® AF (OG)	214:230	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	2	66	theme	blood	468:472	arg1	cells					486:490	peripheral blood mononuclear cells	457:490	peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	457:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	2	66	theme	blood	468:472	arg1	PBMC					493:496	PBMC	493:496	PBMC	493:496	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	11	67	theme	similar	1505:1511	arg1	incidence					1521:1529	similar disease incidence	1505:1529	similar disease incidence	1505:1529	Prior to the experiment, cows in both treatments had similar disease incidence.
37279587	2	68	from	diet	449:452	arg1	OG					437:438	withdrawing OG	425:438	withdrawing OG from the diet	425:452	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	2	68	from	diet	449:452	arg1	effect					415:420	the effect	411:420	the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	411:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	3	69	theme	Multiparous	542:552	arg1	n					569:569	n = 32	569:574	n = 32	569:574	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	69	theme	Multiparous	542:552	arg1	cows					563:566	Multiparous Holstein cows	542:566	Multiparous Holstein cows (n = 32)	542:575	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	5	70	contain	containing	921:930	arg2	OG					932:933	OG	932:933	OG	932:933	All cows were fed the same diet containing OG for at least one year before the onset of treatments.
37279587	5	70	contain	containing	921:930	arg1	diet					916:919	the same diet	907:919	the same diet containing OG for at least one year	907:955	All cows were fed the same diet containing OG for at least one year before the onset of treatments.
37279587	15	71	theme	greater	1987:1993	arg1	proliferation					1995:2007	greater proliferation	1987:2007	greater proliferation	1987:2007	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	1	72	theme	lactating	323:331	arg1	cows					333:336	lactating cows	323:336	lactating cows	323:336	Supplementation of immunomodulatory feed additives, such as OmniGen ® AF (OG), helps to maintain immune competency; however, it is unknown if immune benefits persist in lactating cows after OG is removed from the diet.
37279587	16	73	theme	withdrawing	2096:2106	arg1	OG					2108:2109	withdrawing OG	2096:2109	withdrawing OG from the diet of mid-lactation cows	2096:2145	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	3	74	theme	dietary	679:685	arg1	treatments					687:696	the two dietary treatments	671:696	the two dietary treatments	671:696	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	14	75	theme	condition	1754:1762	arg1	score					1764:1768	greater body condition score	1741:1768	greater body condition score (2.83 vs. 2.92, P = 0.04)	1741:1794	Compared with CTL, feeding OG maintained greater body condition score (2.83 vs. 2.92, P = 0.04).
37279587	0	76	theme	peripheral	105:114	arg1	cell					134:137	peripheral blood mononuclear cell	105:137	peripheral blood mononuclear cell proliferation	105:151	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.
37279587	12	77	theme	disease	1585:1591	arg1	symptoms					1573:1580	symptoms	1573:1580	symptoms of disease	1573:1591	During the experiment, cows did not show symptoms of disease.
37279587	9	78	theme	treatments	1278:1287	arg1	onset					1269:1273	the onset	1265:1273	the onset of treatments	1265:1287	Blood samples were collected at -1, 1, 3, 5, and 7 wk relative to the onset of treatments for the isolation of PBMC.
37279587	7	79	theme	consecutive	1091:1101	arg1	composition					1123:1133	composition	1123:1133	composition	1123:1133	Milk samples were collected from 3 consecutive milkings weekly and composition analyzed.
37279587	7	79	theme	consecutive	1091:1101	arg1	weekly					1112:1117	weekly	1112:1117	weekly	1112:1117	Milk samples were collected from 3 consecutive milkings weekly and composition analyzed.
37279587	7	79	theme	consecutive	1091:1101	arg1	milkings					1103:1110	3 consecutive milkings weekly and composition	1089:1133	3 consecutive milkings weekly and composition analyzed	1089:1142	Milk samples were collected from 3 consecutive milkings weekly and composition analyzed.
37279587	2	80	from	effect	415:420	arg1	diet					449:452	the diet	445:452	the diet	445:452	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	2	80	from	effect	415:420	arg1	proliferation					499:511	peripheral blood mononuclear cells (PBMC) proliferation	457:511	peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows	457:539	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	14	81	theme	greater	1741:1747	arg1	score					1764:1768	greater body condition score	1741:1768	greater body condition score (2.83 vs. 2.92, P = 0.04)	1741:1794	Compared with CTL, feeding OG maintained greater body condition score (2.83 vs. 2.92, P = 0.04).
37279587	3	82	from	cows	563:566	arg1	d					635:635	153 ± 39 d	626:635	153 ± 39 d	626:635	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	3	82	from	cows	563:566	arg1	milk					620:623	milk	620:623	milk (153 ± 39 d)	620:636	Multiparous Holstein cows (n = 32), blocked by parity (2.7 ± 0.8) and days in milk (153 ± 39 d) were randomly assigned to one of the two dietary treatments within each block: diets were top dressed with either OG (56 g/d/cow) or placebo (CTL, 56 g/d/cow).
37279587	2	83	theme	trial	389:393	arg1	aim					377:379	The aim	373:379	The aim of this trial	373:393	The aim of this trial was to evaluate the effect of withdrawing OG from the diet on peripheral blood mononuclear cells (PBMC) proliferation of mid-lactation dairy cows.
37279587	15	84	dep	stimulated	2014:2023	arg1	index					2048:2052	stimulation index	2036:2052	stimulation index	2036:2052	Regardless of time, relative to CTL, PBMC isolated from cows fed OG had a greater proliferative rate when stimulated with LPS (stimulation index: 1.27 vs. 1.80, P = 0.05) and tended to have greater proliferation when stimulated with ConA (stimulation index: 5.24 vs. 7.80, P = 0.08).
37279587	13	85	dep	yield	1654:1658	arg1	≥					1641:1641	≥	1641:1641	≥	1641:1641	Withdrawing OG from the diet did not affect (P ≥ 0.20) milk yield or composition, intake, or body weight.
37279587	7	86	theme	Milk	1056:1059	arg1	samples					1061:1067	Milk samples	1056:1067	Milk samples	1056:1067	Milk samples were collected from 3 consecutive milkings weekly and composition analyzed.
37279587	14	87	dep	=	1788:1788	arg1	2.92					1780:1783	2.92	1780:1783	2.92	1780:1783	Compared with CTL, feeding OG maintained greater body condition score (2.83 vs. 2.92, P = 0.04).
37279587	14	87	dep	=	1788:1788	arg1	2.83					1771:1774	2.83	1771:1774	2.83	1771:1774	Compared with CTL, feeding OG maintained greater body condition score (2.83 vs. 2.92, P = 0.04).
37279587	8	88	theme	condition	1161:1169	arg1	score					1171:1175	condition score	1161:1175	condition score	1161:1175	Body weight and condition score were measured weekly.
37279587	16	89	theme	PBMC	2181:2184	arg1	response					2169:2176	proliferative response	2155:2176	proliferative response of PBMC	2155:2184	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	4	90	theme	Calan	876:880	arg1	gates					882:886	Calan gates	876:886	Calan gates	876:886	Cows were housed in the same free-stall pen and individually fed 1×/d through Calan gates.
37279587	13	91	theme	milk	1649:1652	arg1	yield					1654:1658	(P ≥ 0.20) milk yield	1638:1658	(P ≥ 0.20) milk yield	1638:1658	Withdrawing OG from the diet did not affect (P ≥ 0.20) milk yield or composition, intake, or body weight.
37279587	0	92	dep	Communication	6:18	arg1	Effect					21:26	Effect	21:26	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.	0:152	Short Communication: Effect of withdrawing an immunomodulatory feed additive from lactating cow diets on peripheral blood mononuclear cell proliferation.
37279587	16	93	theme	immunomodulatory	2206:2221	arg1	role					2223:2226	the immunomodulatory role	2202:2226	the immunomodulatory role of OG	2202:2232	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	16	93	theme	immunomodulatory	2206:2221	arg1	week					2258:2261	one week	2254:2261	one week after its withdrawal from the diet of lactating dairy cows	2254:2320	In conclusion, withdrawing OG from the diet of mid-lactation cows reduced proliferative response of PBMC suggesting that the immunomodulatory role of OG is lost as early as one week after its withdrawal from the diet of lactating dairy cows.
37279587	10	94	theme	proliferative	1427:1439	arg1	responses					1441:1449	proliferative responses	1427:1449	proliferative responses	1427:1449	The PBMC were cultured with concanavalin A (ConA) and lipopolysaccharides (LPS) for 72 h in vitro to determine proliferative responses.
36397114	14	0	theme	Lactobacillus_hayakitensis	2557:2582	arg1	abundance					2495:2503	the relative abundance	2482:2503	the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis	2482:2582	Particularly, oats fed to the foals significantly increased the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis.
36397114	7	1	from	curves	1243:1248	arg1	foals					1335:1339	all foals	1331:1339	all foals	1331:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	4	2	theme	grains	824:829	arg1	effect					808:813	the effect	804:813	the mechanism through which these ingested grains are chemically digested as well as the effect of these grains on the stomach	719:844	However, the mechanism through which these ingested grains are chemically digested as well as the effect of these grains on the stomach remains elusive.
36397114	4	2	theme	grains	824:829	arg1	mechanism					723:731	the mechanism	719:731	the mechanism through which these ingested grains are chemically digested as well as the effect of these grains on the stomach	719:844	However, the mechanism through which these ingested grains are chemically digested as well as the effect of these grains on the stomach remains elusive.
36397114	5	3	from	effects	887:893	arg1	structure					972:980	structure	972:980	structure	972:980	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	3	from	effects	887:893	arg1	composition					987:997	composition	987:997	composition	987:997	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	3	from	effects	887:893	arg1	diversity					961:969	bacterial diversity	951:969	bacterial diversity	951:969	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	7	4	theme	microbiota	1317:1326	arg1	OTUs					1282:1285	OTUs	1282:1285	OTUs	1282:1285	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	7	4	theme	microbiota	1317:1326	arg1	diversity					1292:1300	diversity	1292:1300	diversity of the stomach microbiota in all foals	1292:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	7	4	theme	microbiota	1317:1326	arg1	Units					1275:1279	Operational Taxonomic Units	1253:1279	Operational Taxonomic Units (OTUs)	1253:1286	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	3	5	theme	microbial	654:662	arg1	fermentation					664:675	their microbial fermentation	648:675	their microbial fermentation into Volatile fatty acids (VFA)	648:707	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	1	6	from	microorganisms	258:271	arg1	stomach					280:286	the stomach	276:286	the stomach	276:286	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	15	7	theme	enzyme	2682:2687	arg1	viability					2689:2697	enzyme viability	2682:2697	enzyme viability	2682:2697	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	1	8	theme	cattle	177:182	arg1	BACKGROUND					128:137	BACKGROUND	128:137	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse	128:204	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	2	9	theme	equine	494:499	arg1	stomach					501:507	the equine stomach	490:507	the equine stomach	490:507	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	7	10	theme	diversity	1292:1300	arg1	curves					1243:1248	the bacterial rarefaction curves	1217:1248	the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals	1217:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	15	11	contain	had	2595:2597	arg2	effect					2614:2619	no significant effect	2599:2619	no significant effect	2599:2619	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	15	11	contain	had	2595:2597	arg1	grain					2589:2593	The grain	2585:2593	The grain	2585:2593	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	3	12	theme	fatty	691:695	arg1	VFA					704:706	VFA	704:706	VFA	704:706	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	3	12	theme	fatty	691:695	arg1	acids					697:701	Volatile fatty acids	682:701	Volatile fatty acids (VFA)	682:707	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	5	13	dep	grains	916:921	arg1	corn					924:927	corn	924:927	corn	924:927	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	13	dep	grains	916:921	arg1	grains					916:921	feeding different grains	898:921	feeding different grains (corn, oats, and barley)	898:946	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	13	dep	grains	916:921	arg1	oats					930:933	oats	930:933	oats	930:933	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	13	dep	grains	916:921	arg1	barley					940:945	barley	940:945	barley	940:945	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	1	14	theme	environment	358:368	arg1	homeostasis					330:340	the homeostasis	326:340	the homeostasis of the internal environment	326:368	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	5	15	from	composition	987:997	arg1	content					1021:1027	the foal's stomach content	1002:1027	the foal's stomach content	1002:1027	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	11	16	theme	glucose	2146:2152	arg1	concentration					2154:2166	glucose concentration	2146:2166	glucose concentration	2146:2166	The study also revealed statistical differences for pH, glucose concentration, β-amylase, maltase, and amylase.
36397114	6	17	theme	stomach	1119:1125	arg1	enzymes					1127:1133	starch digestion-related stomach enzymes	1094:1133	starch digestion-related stomach enzymes	1094:1133	Furthermore, the effects of different grains on the vitality of starch digestion-related stomach enzymes were investigated.
36397114	8	18	dep	Streptococcaceae	1475:1490	arg1	Actinobacteria					1426:1439	Actinobacteria	1426:1439	Actinobacteria	1426:1439	This study also revealed the statistical differences for Firmicutes, Cyanobacteria, Actinobacteria, Fibrobacteres, Lactobacillaceae, Streptococcaceae, Unidentified_Clostridiales, Prevotellaceae, Lactobacillus, Streptococcus, Unidentified_Cyanobacteria, Unidentified_Clostridiales, Lactococcus, Sphingomonas, Lactobacillus_hayakitensis, Lactobacillus_equigenerosi, and Clostridium_perfringens.
36397114	8	18	dep	Streptococcaceae	1475:1490	arg1	Cyanobacteria					1411:1423	Firmicutes, Cyanobacteria, Actinobacteria, Fibrobacteres, Lactobacillaceae, Streptococcaceae	1399:1490	Cyanobacteria	1411:1423	This study also revealed the statistical differences for Firmicutes, Cyanobacteria, Actinobacteria, Fibrobacteres, Lactobacillaceae, Streptococcaceae, Unidentified_Clostridiales, Prevotellaceae, Lactobacillus, Streptococcus, Unidentified_Cyanobacteria, Unidentified_Clostridiales, Lactococcus, Sphingomonas, Lactobacillus_hayakitensis, Lactobacillus_equigenerosi, and Clostridium_perfringens.
36397114	3	19	theme	main	525:528	arg1	source					537:542	the main energy source	521:542	the main energy source for plant-eating livestock	521:569	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	3	19	theme	main	525:528	arg1	Grains					510:515	Grains	510:515	Grains	510:515	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	7	20	from	diversity	1292:1300	arg1	foals					1335:1339	all foals	1331:1339	all foals	1331:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	9	21	theme	discriminant	1746:1757	arg1	analysis					1759:1766	linear discriminant analysis	1739:1766	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis revealed 9 bacteria at each classification level.
36397114	2	22	from	diversity	451:459	arg1	stomach					501:507	the equine stomach	490:507	the equine stomach	490:507	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	7	23	theme	Taxonomic	1265:1273	arg1	OTUs					1282:1285	OTUs	1282:1285	OTUs	1282:1285	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	7	23	theme	Taxonomic	1265:1273	arg1	Units					1275:1279	Operational Taxonomic Units	1253:1279	Operational Taxonomic Units (OTUs)	1253:1286	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	5	24	theme	bacterial	951:959	arg1	diversity					961:969	bacterial diversity	951:969	bacterial diversity	951:969	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	13	25	theme	relative	2337:2344	arg1	abundances					2356:2365	the relative bacterial abundances	2333:2365	the relative bacterial abundances	2333:2365	However, the relative bacterial abundances differed significantly in response to different diets.
36397114	9	26	theme	effect	1768:1773	arg1	analysis					1780:1787	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis revealed 9 bacteria at each classification level.
36397114	0	27	from	digestion	87:95	arg1	stomach					119:125	the foal stomach	110:125	the foal stomach	110:125	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	5	28	from	diversity	961:969	arg1	content					1021:1027	the foal's stomach content	1002:1027	the foal's stomach content	1002:1027	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	10	29	theme	functional	1843:1852	arg1	analysis					1854:1861	The functional analysis	1839:1861	The functional analysis of species information by using FAPROTAX software	1839:1911	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	10	29	theme	functional	1843:1852	arg1	able					1917:1920	able	1917:1920	able	1917:1920	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	4	30	from	mechanism	723:731	arg1	stomach					838:844	the stomach	834:844	the stomach	834:844	However, the mechanism through which these ingested grains are chemically digested as well as the effect of these grains on the stomach remains elusive.
36397114	0	31	from	Effects	0:6	arg1	diversity					41:49	bacterial diversity	31:49	bacterial diversity	31:49	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	0	31	from	Effects	0:6	arg1	activity					62:69	enzyme activity	55:69	enzyme activity	55:69	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	15	32	from	pH	2628:2629	arg1	foal					2706:2709	the foal	2702:2709	the foal	2702:2709	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	7	33	located	observed	1194:1201	arg2	differences					1177:1187	No significant differences	1162:1187	No significant differences	1162:1187	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	7	33	located	observed	1194:1201	arg1	curves					1243:1248	the bacterial rarefaction curves	1217:1248	the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals	1217:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	15	34	theme	significant	2602:2612	arg1	effect					2614:2619	no significant effect	2599:2619	no significant effect	2599:2619	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	12	35	theme	content	2303:2309	arg1	diversity					2278:2286	the microbial diversity	2264:2286	the microbial diversity of the stomach content of the foal	2264:2321	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	0	36	theme	starch	100:105	arg1	digestion					87:95	digestion	87:95	digestion of starch in the foal stomach	87:125	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	5	37	theme	different	906:914	arg1	corn					924:927	corn	924:927	corn	924:927	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	37	theme	different	906:914	arg1	grains					916:921	feeding different grains	898:921	feeding different grains (corn, oats, and barley)	898:946	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	37	theme	different	906:914	arg1	barley					940:945	barley	940:945	barley	940:945	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	9	38	theme	classification	1817:1830	arg1	level					1832:1836	each classification level	1812:1836	each classification level	1812:1836	The linear discriminant analysis effect size analysis revealed 9 bacteria at each classification level.
36397114	12	39	theme	foal	2318:2321	arg1	content					2303:2309	the stomach content	2291:2309	the stomach content of the foal	2291:2321	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	10	40	theme	top	1955:1957	arg1	chemoheterotrophy					1976:1992	chemoheterotrophy	1976:1992	chemoheterotrophy	1976:1992	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	10	40	theme	top	1955:1957	arg1	functions					1961:1969	the top 5 functions	1951:1969	the top 5 functions	1951:1969	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	8	41	theme	statistical	1371:1381	arg1	differences					1383:1393	the statistical differences	1367:1393	the statistical differences for Firmicutes, Cyanobacteria, Actinobacteria, Fibrobacteres, Lactobacillaceae, Streptococcaceae, Unidentified_Clostridiales, Prevotellaceae, Lactobacillus, Streptococcus, Unidentified_Cyanobacteria, Unidentified_Clostridiales, Lactococcus, Sphingomonas, Lactobacillus_hayakitensis, Lactobacillus_equigenerosi, and Clostridium_perfringens	1367:1732	This study also revealed the statistical differences for Firmicutes, Cyanobacteria, Actinobacteria, Fibrobacteres, Lactobacillaceae, Streptococcaceae, Unidentified_Clostridiales, Prevotellaceae, Lactobacillus, Streptococcus, Unidentified_Cyanobacteria, Unidentified_Clostridiales, Lactococcus, Sphingomonas, Lactobacillus_hayakitensis, Lactobacillus_equigenerosi, and Clostridium_perfringens.
36397114	7	42	theme	bacterial	1221:1229	arg1	curves					1243:1248	the bacterial rarefaction curves	1217:1248	the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals	1217:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	0	43	theme	different	11:19	arg1	grains					21:26	different grains	11:26	different grains	11:26	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	2	44	dep	diversity	451:459	arg1	the					447:449	the	447:449	the	447:449	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	0	45	theme	enzyme	55:60	arg1	activity					62:69	enzyme activity	55:69	enzyme activity	55:69	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	4	46	theme	ingested	753:760	arg1	grains					762:767	these ingested grains	747:767	these ingested grains	747:767	However, the mechanism through which these ingested grains are chemically digested as well as the effect of these grains on the stomach remains elusive.
36397114	12	47	contain	had	2235:2237	arg2	effect					2254:2259	no significant effect	2239:2259	no significant effect	2239:2259	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	12	47	contain	had	2235:2237	arg1	grains					2228:2233	The different grains	2214:2233	The different grains	2214:2233	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	10	48	theme	information	1874:1884	arg1	analysis					1854:1861	The functional analysis	1839:1861	The functional analysis of species information by using FAPROTAX software	1839:1911	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	10	48	theme	information	1874:1884	arg1	able					1917:1920	able	1917:1920	able	1917:1920	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	3	49	theme	plant-eating	548:559	arg1	livestock					561:569	plant-eating livestock	548:569	plant-eating livestock	548:569	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	3	50	theme	grains	625:630	arg1	hydrolysis					611:620	enzymatic hydrolysis	601:620	enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA)	601:707	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	14	51	theme	relative	2486:2493	arg1	abundance					2495:2503	the relative abundance	2482:2503	the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis	2482:2582	Particularly, oats fed to the foals significantly increased the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis.
36397114	15	52	theme	concentration	2663:2675	arg1	pH					2628:2629	the pH	2624:2629	the pH of the stomach content, glucose concentration, and enzyme viability in the foal	2624:2709	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	1	53	theme	horse	200:204	arg1	cattle					177:182	ruminant cattle	168:182	ruminant cattle	168:182	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	1	53	theme	horse	200:204	arg1	stomach					189:195	the stomach	185:195	the stomach of horse	185:204	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	13	54	theme	different	2405:2413	arg1	diets					2415:2419	different diets	2405:2419	different diets	2405:2419	However, the relative bacterial abundances differed significantly in response to different diets.
36397114	2	55	from	complexity	382:391	arg1	stomach					416:422	the stomach	412:422	the stomach	412:422	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	15	56	theme	stomach	2638:2644	arg1	content					2646:2652	the stomach content	2634:2652	the stomach content	2634:2652	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	4	57	from	effect	808:813	arg1	stomach					838:844	the stomach	834:844	the stomach	834:844	However, the mechanism through which these ingested grains are chemically digested as well as the effect of these grains on the stomach remains elusive.
36397114	1	58	theme	chemical	230:237	arg1	digestion					239:247	chemical digestion	230:247	chemical digestion	230:247	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	14	59	theme	Lactobacillus	2538:2550	arg1	abundance					2495:2503	the relative abundance	2482:2503	the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis	2482:2582	Particularly, oats fed to the foals significantly increased the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis.
36397114	6	60	theme	grains	1068:1073	arg1	effects					1047:1053	the effects	1043:1053	the effects of different grains on the vitality of starch digestion-related stomach enzymes	1043:1133	Furthermore, the effects of different grains on the vitality of starch digestion-related stomach enzymes were investigated.
36397114	3	61	theme	Volatile	682:689	arg1	VFA					704:706	VFA	704:706	VFA	704:706	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	3	61	theme	Volatile	682:689	arg1	acids					697:701	Volatile fatty acids	682:701	Volatile fatty acids (VFA)	682:707	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	2	62	from	structure	465:473	arg1	stomach					501:507	the equine stomach	490:507	the equine stomach	490:507	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	14	63	theme	Lactobacillaceae	2520:2535	arg1	abundance					2495:2503	the relative abundance	2482:2503	the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis	2482:2582	Particularly, oats fed to the foals significantly increased the relative abundance of Firmicutes, Lactobacillaceae, Lactobacillus, and Lactobacillus_hayakitensis.
36397114	1	64	theme	ruminant	168:175	arg1	cattle					177:182	ruminant cattle	168:182	ruminant cattle	168:182	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	1	64	theme	ruminant	168:175	arg1	stomach					189:195	the stomach	185:195	the stomach of horse	185:204	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	7	65	theme	stomach	1309:1315	arg1	microbiota					1317:1326	the stomach microbiota	1305:1326	the stomach microbiota	1305:1326	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	0	66	theme	foal	114:117	arg1	stomach					119:125	the foal stomach	110:125	the foal stomach	110:125	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	15	67	theme	viability	2689:2697	arg1	pH					2628:2629	the pH	2624:2629	the pH of the stomach content, glucose concentration, and enzyme viability in the foal	2624:2709	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	13	68	theme	bacterial	2346:2354	arg1	abundances					2356:2365	the relative bacterial abundances	2333:2365	the relative bacterial abundances	2333:2365	However, the relative bacterial abundances differed significantly in response to different diets.
36397114	1	69	theme	internal	349:356	arg1	environment					358:368	the internal environment	345:368	the internal environment	345:368	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	3	70	theme	energy	530:535	arg1	source					537:542	the main energy source	521:542	the main energy source for plant-eating livestock	521:569	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	3	70	theme	energy	530:535	arg1	Grains					510:515	Grains	510:515	Grains	510:515	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	12	71	dep	CONCLUSIONS	2202:2212	arg1	had					2235:2237	had	2235:2237	had no significant effect on the microbial diversity of the stomach content of the foal	2235:2321	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	2	72	theme	bacteria	478:485	arg1	structure					465:473	structure	465:473	structure	465:473	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	2	72	theme	bacteria	478:485	arg1	diversity					451:459	diversity	451:459	diversity	451:459	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	6	73	theme	enzymes	1127:1133	arg1	vitality					1082:1089	the vitality	1078:1089	the vitality of starch digestion-related stomach enzymes	1078:1133	Furthermore, the effects of different grains on the vitality of starch digestion-related stomach enzymes were investigated.
36397114	9	74	theme	linear	1739:1744	arg1	analysis					1759:1766	linear discriminant analysis	1739:1766	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis revealed 9 bacteria at each classification level.
36397114	6	75	theme	digestion-related	1101:1117	arg1	enzymes					1127:1133	starch digestion-related stomach enzymes	1094:1133	starch digestion-related stomach enzymes	1094:1133	Furthermore, the effects of different grains on the vitality of starch digestion-related stomach enzymes were investigated.
36397114	10	76	theme	species	1866:1872	arg1	information					1874:1884	species information	1866:1884	species information	1866:1884	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	9	77	theme	analysis	1759:1766	arg1	analysis					1780:1787	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis revealed 9 bacteria at each classification level.
36397114	7	78	theme	Units	1275:1279	arg1	curves					1243:1248	the bacterial rarefaction curves	1217:1248	the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals	1217:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	9	79	theme	size	1775:1778	arg1	analysis					1780:1787	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis	1735:1787	The linear discriminant analysis effect size analysis revealed 9 bacteria at each classification level.
36397114	7	80	theme	Operational	1253:1263	arg1	OTUs					1282:1285	OTUs	1282:1285	OTUs	1282:1285	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	7	80	theme	Operational	1253:1263	arg1	Units					1275:1279	Operational Taxonomic Units	1253:1279	Operational Taxonomic Units (OTUs)	1253:1286	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	1	81	theme	important	296:304	arg1	role					306:309	an important role	293:309	an important role	293:309	BACKGROUND Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment.
36397114	7	82	from	foals	1335:1339	arg1	curves					1243:1248	the bacterial rarefaction curves	1217:1248	the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals	1217:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	5	83	theme	feeding	898:904	arg1	corn					924:927	corn	924:927	corn	924:927	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	83	theme	feeding	898:904	arg1	grains					916:921	feeding different grains	898:921	feeding different grains (corn, oats, and barley)	898:946	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	5	83	theme	feeding	898:904	arg1	barley					940:945	barley	940:945	barley	940:945	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	7	84	theme	rarefaction	1231:1241	arg1	curves					1243:1248	the bacterial rarefaction curves	1217:1248	the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals	1217:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	12	85	theme	stomach	2295:2301	arg1	content					2303:2309	the stomach content	2291:2309	the stomach content of the foal	2291:2321	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	7	86	from	Units	1275:1279	arg1	foals					1335:1339	all foals	1331:1339	all foals	1331:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	2	87	theme	microbes	400:407	arg1	complexity					382:391	the complexity	378:391	the complexity of the microbes in the stomach	378:422	Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach.
36397114	0	88	theme	grains	21:26	arg1	Effects					0:6	Effects	0:6	Effects of different grains on bacterial diversity and enzyme activity	0:69	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	7	89	dep	RESULTS	1154:1160	arg1	observed					1194:1201	observed	1194:1201	were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals	1189:1339	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	5	90	theme	stomach	1013:1019	arg1	content					1021:1027	the foal's stomach content	1002:1027	the foal's stomach content	1002:1027	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	10	91	theme	FAPROTAX	1895:1902	arg1	software					1904:1911	FAPROTAX software	1895:1911	FAPROTAX software	1895:1911	The functional analysis of species information by using FAPROTAX software was able to predict 35 functions, and the top 5 functions were chemoheterotrophy, fermentation, animal_parasites_or_symbionts, nitrate_reduction, and aerobic_chemoheterotrophy.
36397114	12	92	theme	microbial	2268:2276	arg1	diversity					2278:2286	the microbial diversity	2264:2286	the microbial diversity of the stomach content of the foal	2264:2321	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	6	93	from	effects	1047:1053	arg1	vitality					1082:1089	the vitality	1078:1089	the vitality of starch digestion-related stomach enzymes	1078:1133	Furthermore, the effects of different grains on the vitality of starch digestion-related stomach enzymes were investigated.
36397114	0	94	theme	bacterial	31:39	arg1	diversity					41:49	bacterial diversity	31:49	bacterial diversity	31:49	Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
36397114	5	95	from	structure	972:980	arg1	content					1021:1027	the foal's stomach content	1002:1027	the foal's stomach content	1002:1027	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	11	96	theme	statistical	2114:2124	arg1	differences					2126:2136	statistical differences	2114:2136	statistical differences for pH, glucose concentration, β-amylase, maltase, and amylase	2114:2199	The study also revealed statistical differences for pH, glucose concentration, β-amylase, maltase, and amylase.
36397114	12	97	theme	significant	2242:2252	arg1	effect					2254:2259	no significant effect	2239:2259	no significant effect	2239:2259	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	15	98	theme	glucose	2655:2661	arg1	concentration					2663:2675	glucose concentration	2655:2675	glucose concentration	2655:2675	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	5	99	theme	grains	916:921	arg1	effects					887:893	the effects	883:893	the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content	883:1027	This study explored the effects of feeding different grains (corn, oats, and barley) on bacterial diversity, structure, and composition in the foal's stomach content.
36397114	7	100	theme	significant	1165:1175	arg1	differences					1177:1187	No significant differences	1162:1187	No significant differences	1162:1187	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	7	101	dep	observed	1194:1201	arg1	P > 0.05					1204:1211	P > 0.05	1204:1211	P > 0.05	1204:1211	RESULTS No significant differences were observed (P > 0.05) in the bacterial rarefaction curves of Operational Taxonomic Units (OTUs) and diversity of the stomach microbiota in all foals.
36397114	3	102	theme	enzymatic	601:609	arg1	hydrolysis					611:620	enzymatic hydrolysis	601:620	enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA)	601:707	Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA).
36397114	15	103	theme	content	2646:2652	arg1	pH					2628:2629	the pH	2624:2629	the pH of the stomach content, glucose concentration, and enzyme viability in the foal	2624:2709	The grain had no significant effect on the pH of the stomach content, glucose concentration, and enzyme viability in the foal.
36397114	6	104	theme	different	1058:1066	arg1	grains					1068:1073	different grains	1058:1073	different grains	1058:1073	Furthermore, the effects of different grains on the vitality of starch digestion-related stomach enzymes were investigated.
36397114	12	105	theme	different	2218:2226	arg1	grains					2228:2233	The different grains	2214:2233	The different grains	2214:2233	CONCLUSIONS The different grains had no significant effect on the microbial diversity of the stomach content of the foal.
36397114	6	106	theme	starch	1094:1099	arg1	enzymes					1127:1133	starch digestion-related stomach enzymes	1094:1133	starch digestion-related stomach enzymes	1094:1133	Furthermore, the effects of different grains on the vitality of starch digestion-related stomach enzymes were investigated.
35068268	4	0	theme	eye	865:867	arg1	model					869:873	a rabbit's eye model	854:873	a rabbit's eye model	854:873	Healing of corneal epithelium ulcers was investigated in a rabbit's eye model.
35068268	3	1	from	properties	648:657	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	2	theme	conjunctival	707:718	arg1	METHODS					533:539	METHODS	533:539	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation	533:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	2	theme	conjunctival	707:718	arg1	potential					731:739	conjunctival irritation potential	707:739	conjunctival irritation potential	707:739	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	2	3	theme	study	266:270	arg1	aim					254:256	The aim	250:256	The aim of this study	250:270	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	4	theme	polymer	306:312	arg1	poloxamer					314:322	the thermo-gelling polymer poloxamer 407	287:326	the thermo-gelling polymer poloxamer 407	287:326	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	3	5	theme	in vitro	742:749	arg1	METHODS					533:539	METHODS	533:539	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation	533:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	5	theme	in vitro	742:749	arg1	release					751:757	in vitro release	742:757	in vitro release	742:757	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	8	6	theme	poloxamer-chitosan	1490:1507	arg1	combinations					1474:1485	combinations	1474:1485	combinations of poloxamer-chitosan	1474:1507	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	8	7	theme	superior	1519:1526	arg1	time					1537:1540	superior gelation time	1519:1540	superior gelation time	1519:1540	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	7	8	theme	poloxamer	1396:1404	arg1	systems					1419:1425	poloxamer 407/chitosan systems	1396:1425	poloxamer 407/chitosan systems	1396:1425	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	3	9	theme	ex	764:765	arg1	METHODS					533:539	METHODS	533:539	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation	533:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	9	theme	ex	764:765	arg1	permeation					772:781	ex vivo permeation	764:781	ex vivo permeation	764:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	potential					731:739	conjunctival irritation potential	707:739	conjunctival irritation potential	707:739	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	effect					545:550	The effect	541:550	The effect of different polymer combinations on parameters like gelation time and temperature	541:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	coefficients					679:690	spreading coefficients	669:690	spreading coefficients	669:690	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	properties					648:657	rheological properties	636:657	rheological properties	636:657	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	permeation					772:781	ex vivo permeation	764:781	ex vivo permeation	764:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	mucoadhesion					693:704	mucoadhesion	693:704	mucoadhesion	693:704	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	release					751:757	in vitro release	742:757	in vitro release	742:757	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	texture					660:666	texture	660:666	texture	660:666	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	10	theme	combinations	573:584	arg1	METHODS					533:539	METHODS	533:539	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation	533:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	1	11	theme	in	139:140	arg1	gels					147:150	Thermosensitive in situ gels	123:150	PURPOSE Thermosensitive in situ gels	115:150	PURPOSE Thermosensitive in situ gels have been around for decades but only a few have been translated into ophthalmic pharmaceuticals.
35068268	3	12	from	effect	545:550	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	potential					731:739	conjunctival irritation potential	707:739	conjunctival irritation potential	707:739	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	effect					545:550	The effect	541:550	The effect of different polymer combinations on parameters like gelation time and temperature	541:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	coefficients					679:690	spreading coefficients	669:690	spreading coefficients	669:690	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	properties					648:657	rheological properties	636:657	rheological properties	636:657	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	permeation					772:781	ex vivo permeation	764:781	ex vivo permeation	764:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	mucoadhesion					693:704	mucoadhesion	693:704	mucoadhesion	693:704	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	release					751:757	in vitro release	742:757	in vitro release	742:757	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	texture					660:666	texture	660:666	texture	660:666	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	13	dep	METHODS	533:539	arg1	METHODS					533:539	METHODS	533:539	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation	533:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	2	14	theme	dipeptide	490:498	arg1	drug					500:503	a natural dipeptide drug	480:503	a natural dipeptide drug	480:503	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	14	theme	dipeptide	490:498	arg1	L-carnosine					467:477	L-carnosine	467:477	L-carnosine (a natural dipeptide drug)	467:504	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	0	15	theme	corneal	70:76	arg1	healing					84:90	corneal wound healing	70:90	enhanced corneal wound healing effect of L-carnosine	61:112	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	3	16	theme	gelation	605:612	arg1	time					614:617	gelation time	605:617	gelation time	605:617	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	0	17	theme	healing	84:90	arg1	effect					92:97	enhanced corneal wound healing effect	61:97	enhanced corneal wound healing effect of L-carnosine	61:112	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	8	18	dep	CONCLUSION	1428:1437	arg1	situ					1442:1445	situ	1442:1445	situ	1442:1445	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	2	19	theme	corneal	510:516	arg1	healing					524:530	corneal wound healing	510:530	corneal wound healing	510:530	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	3	20	from	permeation	772:781	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	21	dep	ex	764:765	arg1	vivo					767:770	vivo	767:770	vivo	767:770	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	5	22	theme	poloxamer	973:981	arg1	concentrations					955:968	the concentrations	951:968	the concentrations	951:968	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	7	23	theme	wound	1364:1368	arg1	healing					1370:1376	enhanced corneal epithelium wound healing	1336:1376	enhanced corneal epithelium wound healing	1336:1376	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	2	24	theme	ophthalmic	435:444	arg1	systems					455:461	effective and long-acting ophthalmic delivery systems	409:461	effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing	409:530	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	25	theme	methyl	372:377	arg1	cellulose					379:387	methyl cellulose	372:387	methyl cellulose	372:387	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	7	26	theme	corneal	1345:1351	arg1	healing					1370:1376	enhanced corneal epithelium wound healing	1336:1376	enhanced corneal epithelium wound healing	1336:1376	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	8	27	theme	long-acting	1628:1638	arg1	systems					1654:1660	effective long-acting drug delivery systems	1618:1660	effective long-acting drug delivery systems for corneal wounds	1618:1679	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	5	28	dep	RESULTS	876:882	arg1	dependent					938:946	dependent	938:946	dependent	938:946	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	4	29	theme	epithelium	816:825	arg1	ulcers					827:832	corneal epithelium ulcers	808:832	corneal epithelium ulcers	808:832	Healing of corneal epithelium ulcers was investigated in a rabbit's eye model.
35068268	0	30	theme	in	36:37	arg1	gels					52:55	in situ forming gels	36:55	in situ forming gels	36:55	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	8	31	theme	delivery	1645:1652	arg1	systems					1654:1660	effective long-acting drug delivery systems	1618:1660	effective long-acting drug delivery systems for corneal wounds	1618:1679	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	8	32	theme	In	1439:1440	arg1	CONCLUSION					1428:1437	CONCLUSION	1428:1437	CONCLUSION	1428:1437	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	0	33	theme	forming	44:50	arg1	gels					52:55	in situ forming gels	36:55	in situ forming gels	36:55	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	5	34	theme	methyl	1023:1028	arg1	cellulose					1030:1038	methyl cellulose	1023:1038	methyl cellulose	1023:1038	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	5	34	theme	methyl	1023:1028	arg1	polymers					1000:1007	additive polymers	991:1007	additive polymers (chitosan and methyl cellulose)	991:1039	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	8	35	theme	gelling	1447:1453	arg1	systems					1455:1461	CONCLUSION In situ gelling systems	1428:1461	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan	1428:1507	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	2	36	theme	polymers	345:352	arg1	CS					364:365	CS	364:365	CS	364:365	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	36	theme	polymers	345:352	arg1	MC					390:391	MC	390:391	MC	390:391	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	36	theme	polymers	345:352	arg1	chitosan					354:361	mucoadhesive polymers chitosan	332:361	mucoadhesive polymers chitosan (CS)	332:366	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	6	37	theme	texture	1132:1138	arg1	analysis					1140:1147	texture analysis	1132:1147	texture analysis (hardness, adhesiveness, and cohesiveness)	1132:1190	Mechanical properties investigated through texture analysis (hardness, adhesiveness, and cohesiveness) were dependent on composition.
35068268	3	38	theme	spreading	669:677	arg1	coefficients					679:690	spreading coefficients	669:690	spreading coefficients	669:690	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	4	39	theme	corneal	808:814	arg1	ulcers					827:832	corneal epithelium ulcers	808:832	corneal epithelium ulcers	808:832	Healing of corneal epithelium ulcers was investigated in a rabbit's eye model.
35068268	5	40	theme	polymers	1000:1007	arg1	concentrations					955:968	the concentrations	951:968	the concentrations	951:968	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	7	41	theme	L-carnosine	1293:1303	arg1	mucoadhesion					1252:1263	mucoadhesion	1252:1263	mucoadhesion	1252:1263	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	7	41	theme	L-carnosine	1293:1303	arg1	healing					1370:1376	enhanced corneal epithelium wound healing	1336:1376	enhanced corneal epithelium wound healing	1336:1376	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	7	41	theme	L-carnosine	1293:1303	arg1	permeation					1279:1288	transcorneal permeation	1266:1288	transcorneal permeation of L-carnosine	1266:1303	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	7	41	theme	L-carnosine	1293:1303	arg1	spreading-ability					1233:1249	Promising spreading-ability	1223:1249	Promising spreading-ability	1223:1249	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	7	41	theme	L-carnosine	1293:1303	arg1	tolerability					1318:1329	high ocular tolerability	1306:1329	high ocular tolerability	1306:1329	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	6	42	dep	analysis	1140:1147	arg1	adhesiveness					1160:1171	adhesiveness	1160:1171	adhesiveness	1160:1171	Mechanical properties investigated through texture analysis (hardness, adhesiveness, and cohesiveness) were dependent on composition.
35068268	6	42	dep	analysis	1140:1147	arg1	cohesiveness					1178:1189	cohesiveness	1178:1189	cohesiveness	1178:1189	Mechanical properties investigated through texture analysis (hardness, adhesiveness, and cohesiveness) were dependent on composition.
35068268	6	42	dep	analysis	1140:1147	arg1	hardness					1150:1157	hardness	1150:1157	hardness	1150:1157	Mechanical properties investigated through texture analysis (hardness, adhesiveness, and cohesiveness) were dependent on composition.
35068268	6	43	theme	Mechanical	1089:1098	arg1	properties					1100:1109	Mechanical properties	1089:1109	Mechanical properties investigated through texture analysis (hardness, adhesiveness, and cohesiveness)	1089:1190	Mechanical properties investigated through texture analysis (hardness, adhesiveness, and cohesiveness) were dependent on composition.
35068268	3	44	theme	irritation	720:729	arg1	METHODS					533:539	METHODS	533:539	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation	533:781	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	44	theme	irritation	720:729	arg1	potential					731:739	conjunctival irritation potential	707:739	conjunctival irritation potential	707:739	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	7	45	theme	Promising	1223:1231	arg1	spreading-ability					1233:1249	Promising spreading-ability	1223:1249	Promising spreading-ability	1223:1249	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	3	46	from	potential	731:739	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	2	47	theme	thermo-gelling	291:304	arg1	poloxamer					314:322	the thermo-gelling polymer poloxamer 407	287:326	the thermo-gelling polymer poloxamer 407	287:326	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	3	48	theme	different	555:563	arg1	combinations					573:584	different polymer combinations	555:584	different polymer combinations	555:584	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	3	49	from	coefficients	679:690	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	7	50	theme	high	1306:1309	arg1	tolerability					1318:1329	high ocular tolerability	1306:1329	high ocular tolerability	1306:1329	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	2	51	theme	wound	518:522	arg1	healing					524:530	corneal wound healing	510:530	corneal wound healing	510:530	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	8	52	theme	gelation	1528:1535	arg1	time					1537:1540	superior gelation time	1519:1540	superior gelation time	1519:1540	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	5	53	dep	polymers	1000:1007	arg1	chitosan					1010:1017	chitosan	1010:1017	chitosan	1010:1017	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	5	53	dep	polymers	1000:1007	arg1	cellulose					1030:1038	methyl cellulose	1023:1038	methyl cellulose	1023:1038	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	5	53	dep	polymers	1000:1007	arg1	polymers					1000:1007	additive polymers	991:1007	additive polymers (chitosan and methyl cellulose)	991:1039	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	3	54	from	mucoadhesion	693:704	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	5	55	theme	several	1072:1078	arg1	minutes					1080:1086	several minutes	1072:1086	several minutes	1072:1086	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	3	56	theme	polymer	565:571	arg1	combinations					573:584	different polymer combinations	555:584	different polymer combinations	555:584	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	1	57	theme	Thermosensitive	123:137	arg1	gels					147:150	Thermosensitive in situ gels	123:150	PURPOSE Thermosensitive in situ gels	115:150	PURPOSE Thermosensitive in situ gels have been around for decades but only a few have been translated into ophthalmic pharmaceuticals.
35068268	7	58	theme	407/chitosan	1406:1417	arg1	systems					1419:1425	poloxamer 407/chitosan systems	1396:1425	poloxamer 407/chitosan systems	1396:1425	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	0	59	theme	wound	78:82	arg1	healing					84:90	corneal wound healing	70:90	enhanced corneal wound healing effect of L-carnosine	61:112	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	2	60	theme	natural	482:488	arg1	drug					500:503	a natural dipeptide drug	480:503	a natural dipeptide drug	480:503	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	60	theme	natural	482:488	arg1	L-carnosine					467:477	L-carnosine	467:477	L-carnosine (a natural dipeptide drug)	467:504	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	1	61	dep	PURPOSE	115:121	arg1	gels					147:150	Thermosensitive in situ gels	123:150	PURPOSE Thermosensitive in situ gels	115:150	PURPOSE Thermosensitive in situ gels have been around for decades but only a few have been translated into ophthalmic pharmaceuticals.
35068268	5	62	theme	gelation	889:896	arg1	time					898:901	gelation time	889:901	gelation time	889:901	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	8	63	theme	rheological	1577:1587	arg1	characteristics					1589:1603	rheological characteristics	1577:1603	rheological characteristics	1577:1603	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	0	64	theme	L-carnosine	102:112	arg1	effect					92:97	enhanced corneal wound healing effect	61:97	enhanced corneal wound healing effect of L-carnosine	61:112	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	0	65	dep	in	36:37	arg1	situ					39:42	situ	39:42	situ	39:42	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	8	66	theme	suitable	1605:1612	arg1	temperature					1546:1556	temperature	1546:1556	temperature	1546:1556	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	7	67	theme	epithelium	1353:1362	arg1	healing					1370:1376	enhanced corneal epithelium wound healing	1336:1376	enhanced corneal epithelium wound healing	1336:1376	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	8	68	theme	effective	1618:1626	arg1	systems					1654:1660	effective long-acting drug delivery systems	1618:1660	effective long-acting drug delivery systems for corneal wounds	1618:1679	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	3	69	from	release	751:757	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	1	70	dep	in	139:140	arg1	situ					142:145	situ	142:145	situ	142:145	PURPOSE Thermosensitive in situ gels have been around for decades but only a few have been translated into ophthalmic pharmaceuticals.
35068268	7	71	theme	enhanced	1336:1343	arg1	healing					1370:1376	enhanced corneal epithelium wound healing	1336:1376	enhanced corneal epithelium wound healing	1336:1376	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	8	72	theme	drug	1640:1643	arg1	systems					1654:1660	effective long-acting drug delivery systems	1618:1660	effective long-acting drug delivery systems for corneal wounds	1618:1679	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	2	73	theme	effective	409:417	arg1	systems					455:461	effective and long-acting ophthalmic delivery systems	409:461	effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing	409:530	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	3	74	theme	rheological	636:646	arg1	properties					648:657	rheological properties	636:657	rheological properties	636:657	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
35068268	7	75	theme	ocular	1311:1316	arg1	tolerability					1318:1329	high ocular tolerability	1306:1329	high ocular tolerability	1306:1329	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	8	76	theme	corneal	1666:1672	arg1	wounds					1674:1679	corneal wounds	1666:1679	corneal wounds	1666:1679	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	8	77	theme	CONCLUSION	1428:1437	arg1	systems					1455:1461	CONCLUSION In situ gelling systems	1428:1461	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan	1428:1507	CONCLUSION In situ gelling systems comprising combinations of poloxamer-chitosan exhibited superior gelation time and temperature, mucoadhesion, and rheological characteristics suitable for effective long-acting drug delivery systems for corneal wounds.
35068268	0	78	theme	enhanced	61:68	arg1	effect					92:97	enhanced corneal wound healing effect	61:97	enhanced corneal wound healing effect of L-carnosine	61:112	Hybrid thermosensitive-mucoadhesive in situ forming gels for enhanced corneal wound healing effect of L-carnosine.
35068268	2	79	theme	delivery	446:453	arg1	systems					455:461	effective and long-acting ophthalmic delivery systems	409:461	effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing	409:530	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	4	80	theme	ulcers	827:832	arg1	Healing					797:803	Healing	797:803	Healing of corneal epithelium ulcers	797:832	Healing of corneal epithelium ulcers was investigated in a rabbit's eye model.
35068268	2	81	theme	mucoadhesive	332:343	arg1	CS					364:365	CS	364:365	CS	364:365	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	81	theme	mucoadhesive	332:343	arg1	MC					390:391	MC	390:391	MC	390:391	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	2	81	theme	mucoadhesive	332:343	arg1	chitosan					354:361	mucoadhesive polymers chitosan	332:361	mucoadhesive polymers chitosan (CS)	332:366	The aim of this study was to combine the thermo-gelling polymer poloxamer 407 and mucoadhesive polymers chitosan (CS) and methyl cellulose (MC) for developing effective and long-acting ophthalmic delivery systems for L-carnosine (a natural dipeptide drug) for corneal wound healing.
35068268	5	82	theme	additive	991:998	arg1	chitosan					1010:1017	chitosan	1010:1017	chitosan	1010:1017	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	5	82	theme	additive	991:998	arg1	cellulose					1030:1038	methyl cellulose	1023:1038	methyl cellulose	1023:1038	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	5	82	theme	additive	991:998	arg1	polymers					1000:1007	additive polymers	991:1007	additive polymers (chitosan and methyl cellulose)	991:1039	RESULTS Both gelation time and temperature were significantly dependent on the concentrations of poloxamer 407 and additive polymers (chitosan and methyl cellulose), where it ranged from <10 s to several minutes.
35068268	1	83	theme	ophthalmic	222:231	arg1	pharmaceuticals					233:247	ophthalmic pharmaceuticals	222:247	ophthalmic pharmaceuticals	222:247	PURPOSE Thermosensitive in situ gels have been around for decades but only a few have been translated into ophthalmic pharmaceuticals.
35068268	7	84	theme	transcorneal	1266:1277	arg1	permeation					1279:1288	transcorneal permeation	1266:1288	transcorneal permeation of L-carnosine	1266:1303	Promising spreading-ability, mucoadhesion, transcorneal permeation of L-carnosine, high ocular tolerability, and enhanced corneal epithelium wound healing were recorded for poloxamer 407/chitosan systems.
35068268	3	85	from	texture	660:666	arg1	parameters					589:598	parameters	589:598	parameters like gelation time and temperature	589:633	METHODS The effect of different polymer combinations on parameters like gelation time and temperature, rheological properties, texture, spreading coefficients, mucoadhesion, conjunctival irritation potential, in vitro release, and ex vivo permeation were studied.
37181283	11	0	theme	chondroitin	1982:1992	arg1	sulfate					1994:2000	chondroitin sulfate	1982:2000	chondroitin sulfate	1982:2000	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	8	1	theme	compounds	1547:1555	arg1	content					1530:1536	the content	1526:1536	the content of these compounds in cartilage	1526:1568	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	3	2	theme	chondroitin	625:635	arg1	sulfate					637:643	chondroitin sulfate	625:643	chondroitin sulfate	625:643	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	4	3	theme	component	825:833	arg1	technique					858:866	the principal component regression calibration technique	811:866	the principal component regression calibration technique	811:866	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	13	4	theme	prolonged	2441:2449	arg1	preparation					2472:2482	prolonged and laborious sample preparation	2441:2482	prolonged and laborious sample preparation	2441:2482	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	1	5	theme	multivariate	240:251	arg1	analysis					265:272	multivariate IR spectral analysis	240:272	multivariate IR spectral analysis	240:272	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	10	6	from	difference	1786:1795	arg1	ratio					1804:1808	the ratio	1800:1808	the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra	1800:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	4	7	theme	TQ	787:788	arg1	software					798:805	the TQ Analyst software	783:805	the TQ Analyst software	783:805	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	10	8	theme	main	1817:1820	arg1	components					1822:1831	the main components	1813:1831	the main components in the matrix of different samples in the absence of obvious changes in the IR spectra	1813:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	12	9	theme	correct	2250:2256	arg1	determination					2258:2270	a correct determination	2248:2270	a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans	2248:2372	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	1	10	theme	quantitative	144:155	arg1	determination					157:169	the quantitative determination	140:169	the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	140:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	6	11	theme	mass	1083:1086	arg1	fractions					1088:1096	the mass fractions	1079:1096	the mass fractions of collagen and chondroitin sulfate	1079:1132	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	10	12	theme	different	1850:1858	arg1	samples					1860:1866	different samples	1850:1866	different samples in the absence of obvious changes in the IR spectra	1850:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	10	13	theme	calibration	1680:1690	arg1	model					1692:1696	a calibration model	1678:1696	a calibration model with a complete analysis of the cartilage IR spectrum	1678:1750	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	8	14	from	change	1368:1373	arg1	ratio					1382:1386	the ratio	1378:1386	the ratio between the relative intensities of the characteristic bands of compounds	1378:1460	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	8	14	from	change	1368:1373	arg1	spectrum					1472:1479	the IR spectrum	1465:1479	the IR spectrum	1465:1479	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	8	15	from	content	1530:1536	arg1	cartilage					1560:1568	cartilage	1560:1568	cartilage	1560:1568	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	1	16	theme	main	178:181	arg1	components					183:192	the main components	174:192	the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	174:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	12	17	theme	component	2213:2221	arg1	method					2234:2239	the principal component regression method	2199:2239	the principal component regression method	2199:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	7	18	theme	chondroitin	1301:1311	arg1	sulfate					1313:1319	chondroitin sulfate	1301:1319	chondroitin sulfate	1301:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	11	19	from	content	1971:1977	arg1	Kchem~5.5					2048:2056	Kchem~5.5	2048:2056	Kchem~5.5	2048:2056	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	11	19	from	content	1971:1977	arg1	cartilage					2024:2032	degraded articular cartilage	2005:2032	degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5)	2005:2057	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	8	20	with	obvious	1484:1490	arg1	change					1516:1521	a significant change	1502:1521	a significant change in the content of these compounds in cartilage	1502:1568	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	4	21	theme	calibration	846:856	arg1	technique					858:866	the principal component regression calibration technique	811:866	the principal component regression calibration technique	811:866	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	7	22	theme	collagen	1288:1295	arg1	spectra					1277:1283	the IR spectra	1270:1283	the IR spectra of collagen and chondroitin sulfate	1270:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	5	23	theme	chondroitin	988:998	arg1	sulfate					1000:1006	chondroitin sulfate	988:1006	chondroitin sulfate	988:1006	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	3	24	theme	IR	540:541	arg1	spectra					543:549	The IR spectra	536:549	The IR spectra of the cartilage samples	536:574	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	13	25	theme	types	2733:2737	arg1	tissue					2713:2718	cartilage tissue	2703:2718	cartilage tissue of different types	2703:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	14	26	theme	tissue	2819:2824	arg1	diagnostics					2794:2804	non-invasive diagnostics	2781:2804	non-invasive diagnostics of cartilage tissue	2781:2824	In future, this approach can be used for non-invasive diagnostics of cartilage tissue.
37181283	11	27	theme	significant	1943:1953	arg1	decrease					1955:1962	a statistically significant decrease	1927:1962	a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5)	1927:2057	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	10	28	theme	IR	1740:1741	arg1	spectrum					1743:1750	the cartilage IR spectrum	1726:1750	the cartilage IR spectrum	1726:1750	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	7	29	theme	spectra	1277:1283	arg1	spectra					1218:1224	The IR spectra	1211:1224	The IR spectra of cartilage tissues	1211:1245	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	7	29	theme	spectra	1277:1283	arg1	superposition					1253:1265	a superposition	1251:1265	a superposition of the IR spectra of collagen and chondroitin sulfate	1251:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	4	30	theme	IR	665:666	arg1	spectra					668:674	The IR spectra	661:674	The IR spectra	661:674	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	10	31	theme	cartilage	1730:1738	arg1	spectrum					1743:1750	the cartilage IR spectrum	1726:1750	the cartilage IR spectrum	1726:1750	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	13	32	theme	samples	2692:2698	arg1	samples					2692:2698	samples	2692:2698	samples of cartilage tissue of different types	2692:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	13	32	theme	samples	2692:2698	arg1	set					2685:2687	a large set	2677:2687	a large set of samples of cartilage tissue of different types	2677:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	6	33	theme	sulfate	1126:1132	arg1	fractions					1088:1096	the mass fractions	1079:1096	the mass fractions of collagen and chondroitin sulfate	1079:1132	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	13	34	theme	chemical	2534:2541	arg1	manipulations					2543:2555	long, multi-stage and laborious chemical manipulations	2502:2555	long, multi-stage and laborious chemical manipulations	2502:2555	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	10	35	theme	obvious	1886:1892	arg1	changes					1894:1900	obvious changes	1886:1900	obvious changes in the IR spectra	1886:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	9	36	dep	0.88±0.05	1636:1644	arg1	4.55					1661:1664	4.55	1661:1664	4.55	1661:1664	This change occurs after trypsinization, when Ksp increases from 0.88±0.05 (Kchem~0.8) to 4.55.
37181283	9	36	dep	0.88±0.05	1636:1644	arg1	to					1658:1659	to	1658:1659	to	1658:1659	This change occurs after trypsinization, when Ksp increases from 0.88±0.05 (Kchem~0.8) to 4.55.
37181283	1	37	theme	classical	305:313	arg1	analysis					324:331	classical chemical analysis	305:331	classical chemical analysis	305:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	3	38	theme	cartilage	558:566	arg1	samples					568:574	the cartilage samples	554:574	the cartilage samples	554:574	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	13	39	theme	cartilage	2703:2711	arg1	tissue					2713:2718	cartilage tissue	2703:2718	cartilage tissue of different types	2703:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	1	40	theme	diagnostic	118:127	arg1	method					129:134	a diagnostic method	116:134	a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	116:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	12	41	theme	express	2152:2158	arg1	analysis					2160:2167	Conclusion IR spectrometric express analysis	2124:2167	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method	2124:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	0	42	theme	Spectra	71:77	arg1	Analysis					56:63	Multivariate Analysis	43:63	Multivariate Analysis of IR Spectra	43:77	Express Analysis of Cartilage Tissue Using Multivariate Analysis of IR Spectra.
37181283	14	43	theme	cartilage	2809:2817	arg1	tissue					2819:2824	cartilage tissue	2809:2824	cartilage tissue	2809:2824	In future, this approach can be used for non-invasive diagnostics of cartilage tissue.
37181283	2	44	theme	defective	481:489	arg1	Methods					348:354	Methods	348:354	Methods	348:354	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	44	theme	defective	481:489	arg1	samples					527:533	samples	527:533	samples	527:533	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	44	theme	defective	481:489	arg1	samples					501:507	trypsinized and defective cartilage samples	465:507	trypsinized and defective cartilage samples	465:507	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	44	theme	defective	481:489	arg1	Materials					334:342	Materials	334:342	Materials	334:342	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	8	45	theme	bands	1443:1447	arg1	intensities					1409:1419	the relative intensities	1396:1419	the relative intensities of the characteristic bands of compounds	1396:1460	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	4	46	theme	attenuated	701:710	arg1	techniques					730:739	the attenuated total reflectance techniques	697:739	the attenuated total reflectance techniques	697:739	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	10	47	theme	IR	1909:1910	arg1	spectra					1912:1918	the IR spectra	1905:1918	the IR spectra	1905:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	10	48	theme	complete	1705:1712	arg1	analysis					1714:1721	a complete analysis	1703:1721	a complete analysis of the cartilage IR spectrum	1703:1750	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	7	49	dep	Results	1203:1209	arg1	reflect					1339:1345	reflect	1339:1345	qualitatively reflect their composition	1325:1363	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	7	49	dep	Results	1203:1209	arg1	spectra					1218:1224	The IR spectra	1211:1224	The IR spectra of cartilage tissues	1211:1245	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	7	49	dep	Results	1203:1209	arg1	superposition					1253:1265	a superposition	1251:1265	a superposition of the IR spectra of collagen and chondroitin sulfate	1251:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	2	50	theme	trypsinized	465:475	arg1	Methods					348:354	Methods	348:354	Methods	348:354	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	50	theme	trypsinized	465:475	arg1	samples					527:533	samples	527:533	samples	527:533	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	50	theme	trypsinized	465:475	arg1	samples					501:507	trypsinized and defective cartilage samples	465:507	trypsinized and defective cartilage samples	465:507	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	50	theme	trypsinized	465:475	arg1	Materials					334:342	Materials	334:342	Materials	334:342	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	8	51	theme	compounds	1452:1460	arg1	bands					1443:1447	the characteristic bands	1424:1447	the characteristic bands of compounds	1424:1460	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	4	52	theme	reflectance	718:728	arg1	techniques					730:739	the attenuated total reflectance techniques	697:739	the attenuated total reflectance techniques	697:739	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	2	53	theme	septum	380:385	arg1	samples					527:533	samples	527:533	samples	527:533	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	53	theme	septum	380:385	arg1	Methods					348:354	Methods	348:354	Methods	348:354	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	53	theme	septum	380:385	arg1	samples					501:507	trypsinized and defective cartilage samples	465:507	trypsinized and defective cartilage samples	465:507	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	53	theme	septum	380:385	arg1	Materials					334:342	Materials	334:342	Materials	334:342	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	10	54	from	samples	1860:1866	arg1	absence					1875:1881	the absence	1871:1881	the absence of obvious changes in the IR spectra	1871:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	11	55	from	decrease	1955:1962	arg1	content					1971:1977	the content	1967:1977	the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5)	1967:2057	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	2	56	theme	nucleus	409:415	arg1	pulposus					417:424	nucleus pulposus	409:424	nucleus pulposus	409:424	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	12	57	theme	IR	2135:2136	arg1	analysis					2160:2167	Conclusion IR spectrometric express analysis	2124:2167	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method	2124:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	13	58	theme	laborious	2524:2532	arg1	manipulations					2543:2555	long, multi-stage and laborious chemical manipulations	2502:2555	long, multi-stage and laborious chemical manipulations	2502:2555	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	0	59	theme	Express	0:6	arg1	Analysis					8:15	Express Analysis	0:15	Express Analysis of Cartilage Tissue	0:35	Express Analysis of Cartilage Tissue Using Multivariate Analysis of IR Spectra.
37181283	1	60	theme	IR	253:254	arg1	analysis					265:272	multivariate IR spectral analysis	240:272	multivariate IR spectral analysis	240:272	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	8	61	from	change	1516:1521	arg1	content					1530:1536	the content	1526:1536	the content of these compounds in cartilage	1526:1568	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	12	62	theme	cartilage	2315:2323	arg1	matrix					2325:2330	the cartilage matrix	2311:2330	the cartilage matrix	2311:2330	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	7	63	theme	tissues	1239:1245	arg1	spectra					1218:1224	The IR spectra	1211:1224	The IR spectra of cartilage tissues	1211:1245	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	7	63	theme	tissues	1239:1245	arg1	superposition					1253:1265	a superposition	1251:1265	a superposition of the IR spectra of collagen and chondroitin sulfate	1251:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	2	64	theme	intervertebral	433:446	arg1	disc					448:451	the intervertebral disc	429:451	the intervertebral disc	429:451	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	3	65	theme	calibration	588:598	arg1	mixtures					600:607	calibration mixtures	588:607	calibration mixtures of collagen and chondroitin sulfate	588:643	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	2	66	theme	knee	388:391	arg1	joint					393:397	knee joint	388:397	knee joint	388:397	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	0	67	theme	Tissue	30:35	arg1	Analysis					8:15	Express Analysis	0:15	Express Analysis of Cartilage Tissue	0:35	Express Analysis of Cartilage Tissue Using Multivariate Analysis of IR Spectra.
37181283	10	68	from	changes	1894:1900	arg1	spectra					1912:1918	the IR spectra	1905:1918	the IR spectra	1905:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	12	69	theme	main	2292:2295	arg1	components					2297:2306	the main components	2288:2306	the main components in the cartilage matrix	2288:2330	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	12	69	theme	main	2292:2295	arg1	those					2333:2337	those	2333:2337	those	2333:2337	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	10	70	theme	spectrum	1743:1750	arg1	analysis					1714:1721	a complete analysis	1703:1721	a complete analysis of the cartilage IR spectrum	1703:1750	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	11	71	theme	degraded	2005:2012	arg1	Kchem~5.5					2048:2056	Kchem~5.5	2048:2056	Kchem~5.5	2048:2056	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	11	71	theme	degraded	2005:2012	arg1	cartilage					2024:2032	degraded articular cartilage	2005:2032	degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5)	2005:2057	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	0	72	theme	Multivariate	43:54	arg1	Analysis					56:63	Multivariate Analysis	43:63	Multivariate Analysis of IR Spectra	43:77	Express Analysis of Cartilage Tissue Using Multivariate Analysis of IR Spectra.
37181283	1	73	theme	data	294:297	arg1	analysis					265:272	multivariate IR spectral analysis	240:272	multivariate IR spectral analysis	240:272	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	1	73	theme	data	294:297	arg1	verification					278:289	verification	278:289	verification of data using classical chemical analysis	278:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	7	74	theme	IR	1215:1216	arg1	spectra					1218:1224	The IR spectra	1211:1224	The IR spectra of cartilage tissues	1211:1245	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	7	74	theme	IR	1215:1216	arg1	superposition					1253:1265	a superposition	1251:1265	a superposition of the IR spectra of collagen and chondroitin sulfate	1251:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	6	75	theme	chondroitin	1114:1124	arg1	sulfate					1126:1132	chondroitin sulfate	1114:1132	chondroitin sulfate	1114:1132	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	1	76	theme	cartilage	197:205	arg1	tissue					207:212	cartilage tissue	197:212	cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	197:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	10	77	from	spectra	1912:1918	arg1	absence					1875:1881	the absence	1871:1881	the absence of obvious changes in the IR spectra	1871:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	5	78	theme	mass	957:960	arg1	fractions					962:970	the mass fractions	953:970	the mass fractions of collagen and chondroitin sulfate	953:1006	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	1	79	theme	types	225:229	arg1	tissue					207:212	cartilage tissue	197:212	cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	197:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	11	80	theme	sulfate	1994:2000	arg1	content					1971:1977	the content	1967:1977	the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5)	1967:2057	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	11	81	theme	articular	2014:2022	arg1	Kchem~5.5					2048:2056	Kchem~5.5	2048:2056	Kchem~5.5	2048:2056	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	11	81	theme	articular	2014:2022	arg1	cartilage					2024:2032	degraded articular cartilage	2005:2032	degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5)	2005:2057	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	3	82	theme	sulfate	637:643	arg1	spectra					543:549	The IR spectra	536:549	The IR spectra of the cartilage samples	536:574	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	3	82	theme	sulfate	637:643	arg1	mixtures					600:607	calibration mixtures	588:607	calibration mixtures of collagen and chondroitin sulfate	588:643	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	12	83	from	ratio	2279:2283	arg1	matrix					2325:2330	the cartilage matrix	2311:2330	the cartilage matrix	2311:2330	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	4	84	theme	principal	815:823	arg1	technique					858:866	the principal component regression calibration technique	811:866	the principal component regression calibration technique	811:866	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	6	85	theme	collagen	1101:1108	arg1	fractions					1088:1096	the mass fractions	1079:1096	the mass fractions of collagen and chondroitin sulfate	1079:1132	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	10	86	theme	components	1822:1831	arg1	ratio					1804:1808	the ratio	1800:1808	the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra	1800:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	6	87	theme	substances	1191:1200	arg1	analysis					1173:1180	the classical chemical analysis	1150:1180	the classical chemical analysis of these substances	1150:1200	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	6	88	theme	fractions	1088:1096	arg1	ratio					1070:1074	the ratio	1066:1074	the ratio of the mass fractions of collagen and chondroitin sulfate	1066:1132	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	11	89	theme	intact	2083:2088	arg1	samples					2090:2096	intact samples	2083:2096	intact samples (Ksp=2.8±1.1; Kchem~2.6)	2083:2121	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	11	89	theme	intact	2083:2088	arg1	Kchem~2.6					2112:2120	Kchem~2.6	2112:2120	Kchem~2.6	2112:2120	Thus, a statistically significant decrease in the content of chondroitin sulfate in degraded articular cartilage (Ksp=4.4±1.8; Kchem~5.5) was shown compared with intact samples (Ksp=2.8±1.1; Kchem~2.6).
37181283	14	90	theme	non-invasive	2781:2792	arg1	diagnostics					2794:2804	non-invasive diagnostics	2781:2804	non-invasive diagnostics of cartilage tissue	2781:2824	In future, this approach can be used for non-invasive diagnostics of cartilage tissue.
37181283	10	91	theme	model	1692:1696	arg1	use					1671:1673	The use	1667:1673	The use of a calibration model with a complete analysis of the cartilage IR spectrum	1667:1750	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	4	92	theme	Analyst	790:796	arg1	software					798:805	the TQ Analyst software	783:805	the TQ Analyst software	783:805	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	13	93	from	changes	2646:2652	arg1	composition					2661:2671	the composition	2657:2671	the composition for a large set of samples of cartilage tissue of different types	2657:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	10	94	from	ratio	1804:1808	arg1	matrix					1840:1845	the matrix	1836:1845	the matrix of different samples in the absence of obvious changes in the IR spectra	1836:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	12	95	theme	regression	2223:2232	arg1	method					2234:2239	the principal component regression method	2199:2239	the principal component regression method	2199:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	13	96	from	features	2633:2640	arg1	composition					2661:2671	the composition	2657:2671	the composition for a large set of samples of cartilage tissue of different types	2657:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	12	97	from	matrix	2325:2330	arg1	ratio					2279:2283	the ratio	2275:2283	the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans	2275:2372	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	1	98	theme	components	183:192	arg1	determination					157:169	the quantitative determination	140:169	the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	140:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	2	99	theme	cartilage	491:499	arg1	Methods					348:354	Methods	348:354	Methods	348:354	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	99	theme	cartilage	491:499	arg1	samples					527:533	samples	527:533	samples	527:533	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	99	theme	cartilage	491:499	arg1	samples					501:507	trypsinized and defective cartilage samples	465:507	trypsinized and defective cartilage samples	465:507	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	99	theme	cartilage	491:499	arg1	Materials					334:342	Materials	334:342	Materials	334:342	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	6	100	theme	classical	1154:1162	arg1	analysis					1173:1180	the classical chemical analysis	1150:1180	the classical chemical analysis of these substances	1150:1200	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	8	101	theme	significant	1504:1514	arg1	change					1516:1521	a significant change	1502:1521	a significant change in the content of these compounds in cartilage	1502:1568	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	13	102	theme	different	2723:2731	arg1	types					2733:2737	different types	2723:2737	different types	2723:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	1	103	theme	study	95:99	arg1	aim					84:86	The aim	80:86	The aim of the study	80:99	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	10	104	from	components	1822:1831	arg1	matrix					1840:1845	the matrix	1836:1845	the matrix of different samples in the absence of obvious changes in the IR spectra	1836:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	4	105	theme	regression	835:844	arg1	technique					858:866	the principal component regression calibration technique	811:866	the principal component regression calibration technique	811:866	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	8	106	theme	relative	1400:1407	arg1	intensities					1409:1419	the relative intensities	1396:1419	the relative intensities of the characteristic bands of compounds	1396:1460	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	10	107	theme	samples	1860:1866	arg1	matrix					1840:1845	the matrix	1836:1845	the matrix of different samples in the absence of obvious changes in the IR spectra	1836:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	12	108	from	components	2297:2306	arg1	matrix					2325:2330	the cartilage matrix	2311:2330	the cartilage matrix	2311:2330	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	5	109	theme	sulfate	1000:1006	arg1	fractions					962:970	the mass fractions	953:970	the mass fractions of collagen and chondroitin sulfate	953:1006	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	3	110	theme	samples	568:574	arg1	spectra					543:549	The IR spectra	536:549	The IR spectra of the cartilage samples	536:574	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	3	110	theme	samples	568:574	arg1	mixtures					600:607	calibration mixtures	588:607	calibration mixtures of collagen and chondroitin sulfate	588:643	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	5	111	theme	calibration	878:888	arg1	dependence					890:899	calibration dependence	878:899	calibration dependence	878:899	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	12	112	theme	principal	2203:2211	arg1	method					2234:2239	the principal component regression method	2199:2239	the principal component regression method	2199:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	10	113	theme	changes	1894:1900	arg1	absence					1875:1881	the absence	1871:1881	the absence of obvious changes in the IR spectra	1871:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	0	114	theme	IR	68:69	arg1	Spectra					71:77	IR Spectra	68:77	IR Spectra	68:77	Express Analysis of Cartilage Tissue Using Multivariate Analysis of IR Spectra.
37181283	8	115	theme	characteristic	1428:1441	arg1	bands					1443:1447	the characteristic bands	1424:1447	the characteristic bands of compounds	1424:1460	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	7	116	theme	IR	1274:1275	arg1	spectra					1277:1283	the IR spectra	1270:1283	the IR spectra of collagen and chondroitin sulfate	1270:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	1	117	theme	chemical	315:322	arg1	analysis					324:331	classical chemical analysis	305:331	classical chemical analysis	305:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	10	118	from	matrix	1840:1845	arg1	ratio					1804:1808	the ratio	1800:1808	the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra	1800:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	10	118	from	matrix	1840:1845	arg1	absence					1875:1881	the absence	1871:1881	the absence of obvious changes in the IR spectra	1871:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	13	119	theme	tissue	2713:2718	arg1	samples					2692:2698	samples	2692:2698	samples of cartilage tissue of different types	2692:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	2	120	theme	disc	448:451	arg1	rib					400:402	rib	400:402	rib	400:402	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	120	theme	disc	448:451	arg1	septum					380:385	the nasal septum	370:385	the nasal septum	370:385	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	120	theme	disc	448:451	arg1	joint					393:397	knee joint	388:397	knee joint	388:397	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	120	theme	disc	448:451	arg1	pulposus					417:424	nucleus pulposus	409:424	nucleus pulposus	409:424	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	12	121	theme	cartilage	2172:2180	arg1	tissue					2182:2187	cartilage tissue	2172:2187	cartilage tissue	2172:2187	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	13	122	theme	long	2502:2505	arg1	manipulations					2543:2555	long, multi-stage and laborious chemical manipulations	2502:2555	long, multi-stage and laborious chemical manipulations	2502:2555	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	13	123	theme	large	2679:2683	arg1	samples					2692:2698	samples	2692:2698	samples of cartilage tissue of different types	2692:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	13	123	theme	large	2679:2683	arg1	set					2685:2687	a large set	2677:2687	a large set of samples of cartilage tissue of different types	2677:2737	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	10	124	with	model	1692:1696	arg1	analysis					1714:1721	a complete analysis	1703:1721	a complete analysis of the cartilage IR spectrum	1703:1750	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	12	125	theme	Conclusion	2124:2133	arg1	analysis					2160:2167	Conclusion IR spectrometric express analysis	2124:2167	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method	2124:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	2	126	dep	Materials	334:342	arg1	Cartilages					356:365	Cartilages	356:365	Cartilages	356:365	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	13	127	theme	multi-stage	2508:2518	arg1	manipulations					2543:2555	long, multi-stage and laborious chemical manipulations	2502:2555	long, multi-stage and laborious chemical manipulations	2502:2555	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	8	128	theme	IR	1469:1470	arg1	spectrum					1472:1479	the IR spectrum	1465:1479	the IR spectrum	1465:1479	A change in the ratio between the relative intensities of the characteristic bands of compounds in the IR spectrum is obvious only with a significant change in the content of these compounds in cartilage.
37181283	4	129	theme	total	712:716	arg1	techniques					730:739	the attenuated total reflectance techniques	697:739	the attenuated total reflectance techniques	697:739	The IR spectra were collected using the attenuated total reflectance techniques, and their processing was performed using the TQ Analyst software and the principal component regression calibration technique.
37181283	2	130	theme	nasal	374:378	arg1	septum					380:385	the nasal septum	370:385	the nasal septum	370:385	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	10	131	from	absence	1875:1881	arg1	spectra					1912:1918	the IR spectra	1905:1918	the IR spectra	1905:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	10	131	from	absence	1875:1881	arg1	matrix					1840:1845	the matrix	1836:1845	the matrix of different samples in the absence of obvious changes in the IR spectra	1836:1918	The use of a calibration model with a complete analysis of the cartilage IR spectrum made it possible to determine the difference in the ratio of the main components in the matrix of different samples in the absence of obvious changes in the IR spectra.
37181283	12	132	theme	tissue	2182:2187	arg1	analysis					2160:2167	Conclusion IR spectrometric express analysis	2124:2167	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method	2124:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	13	133	theme	proposed	2379:2386	arg1	technique					2388:2396	The proposed technique	2375:2396	The proposed technique	2375:2396	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	13	134	theme	laborious	2455:2463	arg1	preparation					2472:2482	prolonged and laborious sample preparation	2441:2482	prolonged and laborious sample preparation	2441:2482	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	0	135	theme	Cartilage	20:28	arg1	Tissue					30:35	Cartilage Tissue	20:35	Cartilage Tissue	20:35	Express Analysis of Cartilage Tissue Using Multivariate Analysis of IR Spectra.
37181283	1	136	theme	spectral	256:263	arg1	analysis					265:272	multivariate IR spectral analysis	240:272	multivariate IR spectral analysis	240:272	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	5	137	theme	Ksp	906:908	arg1	ratio					944:948	the ratio	940:948	the ratio of the mass fractions of collagen and chondroitin sulfate	940:1006	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	5	137	theme	Ksp	906:908	arg1	coefficient					910:920	the Ksp coefficient	902:920	the Ksp coefficient	902:920	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	6	138	theme	chemical	1164:1171	arg1	analysis					1173:1180	the classical chemical analysis	1150:1180	the classical chemical analysis of these substances	1150:1200	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	7	139	theme	cartilage	1229:1237	arg1	tissues					1239:1245	cartilage tissues	1229:1245	cartilage tissues	1229:1245	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	2	140	theme	rib	400:402	arg1	samples					527:533	samples	527:533	samples	527:533	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	140	theme	rib	400:402	arg1	Methods					348:354	Methods	348:354	Methods	348:354	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	140	theme	rib	400:402	arg1	samples					501:507	trypsinized and defective cartilage samples	465:507	trypsinized and defective cartilage samples	465:507	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	140	theme	rib	400:402	arg1	Materials					334:342	Materials	334:342	Materials	334:342	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	141	theme	joint	393:397	arg1	samples					527:533	samples	527:533	samples	527:533	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	141	theme	joint	393:397	arg1	Methods					348:354	Methods	348:354	Methods	348:354	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	141	theme	joint	393:397	arg1	samples					501:507	trypsinized and defective cartilage samples	465:507	trypsinized and defective cartilage samples	465:507	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	141	theme	joint	393:397	arg1	Materials					334:342	Materials	334:342	Materials	334:342	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	5	142	theme	fractions	962:970	arg1	ratio					944:948	the ratio	940:948	the ratio of the mass fractions of collagen and chondroitin sulfate	940:1006	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	5	142	theme	fractions	962:970	arg1	coefficient					910:920	the Ksp coefficient	902:920	the Ksp coefficient	902:920	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	12	143	theme	components	2297:2306	arg1	ratio					2279:2283	the ratio	2275:2283	the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans	2275:2372	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	13	144	theme	sample	2465:2470	arg1	preparation					2472:2482	prolonged and laborious sample preparation	2441:2482	prolonged and laborious sample preparation	2441:2482	The proposed technique includes one measurement, does not require prolonged and laborious sample preparation, does not require long, multi-stage and laborious chemical manipulations to determine each of the components, and makes it possible to determine the features and changes in the composition for a large set of samples of cartilage tissue of different types.
37181283	5	145	theme	collagen	975:982	arg1	fractions					962:970	the mass fractions	953:970	the mass fractions of collagen and chondroitin sulfate	953:1006	Based on calibration dependence, the Ksp coefficient was determined as the ratio of the mass fractions of collagen and chondroitin sulfate.
37181283	12	146	theme	spectrometric	2138:2150	arg1	analysis					2160:2167	Conclusion IR spectrometric express analysis	2124:2167	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method	2124:2239	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	2	147	theme	pulposus	417:424	arg1	samples					527:533	samples	527:533	samples	527:533	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	147	theme	pulposus	417:424	arg1	Methods					348:354	Methods	348:354	Methods	348:354	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	147	theme	pulposus	417:424	arg1	samples					501:507	trypsinized and defective cartilage samples	465:507	trypsinized and defective cartilage samples	465:507	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	2	147	theme	pulposus	417:424	arg1	Materials					334:342	Materials	334:342	Materials	334:342	Materials and Methods Cartilages of the nasal septum, knee joint, rib, and nucleus pulposus of the intervertebral disc, as well as trypsinized and defective cartilage samples, were examined as samples.
37181283	3	148	theme	collagen	612:619	arg1	spectra					543:549	The IR spectra	536:549	The IR spectra of the cartilage samples	536:574	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	3	148	theme	collagen	612:619	arg1	mixtures					600:607	calibration mixtures	588:607	calibration mixtures of collagen and chondroitin sulfate	588:643	The IR spectra of the cartilage samples, as well as calibration mixtures of collagen and chondroitin sulfate, were obtained.
37181283	7	149	theme	sulfate	1313:1319	arg1	spectra					1277:1283	the IR spectra	1270:1283	the IR spectra of collagen and chondroitin sulfate	1270:1319	Results The IR spectra of cartilage tissues are a superposition of the IR spectra of collagen and chondroitin sulfate and qualitatively reflect their composition.
37181283	6	150	theme	equal	1057:1061	arg1	value					1041:1045	the value	1037:1045	the value	1037:1045	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	12	151	theme	ratio	2279:2283	arg1	determination					2258:2270	a correct determination	2248:2270	a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans	2248:2372	Conclusion IR spectrometric express analysis of cartilage tissue employing the principal component regression method allows a correct determination of the ratio of the main components in the cartilage matrix, those of collagen and glycosaminoglycans.
37181283	1	152	theme	tissue	207:212	arg1	components					183:192	the main components	174:192	the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	174:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
37181283	14	153	used	used	2772:2775	arg2	approach					2756:2763	this approach	2751:2763	this approach	2751:2763	In future, this approach can be used for non-invasive diagnostics of cartilage tissue.
37181283	6	154	theme	Kchem	1050:1054	arg1	value					1041:1045	the value	1037:1045	the value	1037:1045	Its value was compared with the value of Kchem, equal to the ratio of the mass fractions of collagen and chondroitin sulfate, obtained using the classical chemical analysis of these substances.
37181283	1	155	theme	various	217:223	arg1	types					225:229	various types	217:229	various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis	217:331	The aim of the study was to develop a diagnostic method for the quantitative determination of the main components of cartilage tissue of various types based on multivariate IR spectral analysis and verification of data using classical chemical analysis.
36342282	18	0	theme	shell	2784:2788	arg1	disease					2790:2796	shell disease	2784:2796	shell disease	2784:2796	Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease.
36342282	13	1	theme	complex	1855:1861	arg1	community					1876:1884	a functional complex epibacterial community	1842:1884	a functional complex epibacterial community on the carapace of C. pagurus	1842:1914	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	8	2	theme	abundant	952:959	arg1	families					972:979	the most abundant and active families	943:979	the most abundant and active families	943:979	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	15	3	from	%	2114:2114	arg1	areas					2188:2192	different North Sea areas	2168:2192	different North Sea areas	2168:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	13	4	theme	isolates	1810:1817	arg1	properties					1792:1801	the physiological properties	1774:1801	the physiological properties of the isolates	1774:1817	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	13	4	theme	isolates	1810:1817	arg1	combination					1726:1736	The combination	1722:1736	The combination of bacterial community analysis	1722:1768	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	11	5	located	observed	1487:1494	arg1	Flavobacteriia					1379:1392	the Flavobacteriia	1375:1392	the Flavobacteriia	1375:1392	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	11	5	located	observed	1487:1494	arg2	chitin					1475:1480	the polysaccharide chitin	1456:1480	the polysaccharide chitin	1456:1480	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	11	5	located	observed	1487:1494	arg2	monomer					1445:1451	the monomer	1441:1451	the monomer	1441:1451	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	16	6	theme	disease	2275:2281	arg1	all					2284:2286	all	2284:2286	all	2284:2286	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	16	6	theme	disease	2275:2281	arg1	forms					2260:2264	different forms	2250:2264	different forms of shell disease	2250:2281	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	8	7	theme	active	965:970	arg1	families					972:979	the most abundant and active families	943:979	the most abundant and active families	943:979	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	9	8	from	areas	1137:1141	arg1	strains					1103:1109	75 bacterial strains	1090:1109	75 bacterial strains	1090:1109	We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing.
36342282	16	9	theme	different	2250:2258	arg1	all					2284:2286	all	2284:2286	all	2284:2286	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	16	9	theme	different	2250:2258	arg1	forms					2260:2264	different forms	2250:2264	different forms of shell disease	2250:2281	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	6	10	theme	black	779:783	arg1	disease					790:796	the black spot disease	775:796	the black spot disease	775:796	This indicates a reduced and shifted microbial community composition caused by the black spot disease.
36342282	2	11	theme	epibacterial	257:268	arg1	community					270:278	the epibacterial community	253:278	the epibacterial community	253:278	However, little is known about concomitant changes in the epibacterial community.
36342282	14	12	theme	shell	1945:1949	arg1	syndrome					1959:1966	shell disease syndrome	1945:1966	shell disease syndrome	1945:1966	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	14	12	theme	shell	1945:1949	arg1	IMPORTANCE					1917:1926	IMPORTANCE	1917:1926	IMPORTANCE In recent years	1917:1942	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	19	13	theme	physiological	2919:2931	arg1	properties					2933:2942	their physiological properties	2913:2942	their physiological properties	2913:2942	Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
36342282	17	14	theme	areas	2423:2427	arg1	communities					2387:2397	the bacterial communities	2373:2397	the bacterial communities of healthy and diseased areas of the shell of C. pagurus	2373:2454	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	13	15	theme	physiological	1778:1790	arg1	properties					1792:1801	the physiological properties	1774:1801	the physiological properties of the isolates	1774:1817	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	19	16	theme	isolates	2829:2836	arg1	Characterization					2799:2814	Characterization	2799:2814	Characterization of bacterial isolates obtained from affected and nonaffected spots	2799:2881	Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
36342282	14	17	from	IMPORTANCE	1917:1926	arg1	years					1938:1942	recent years	1931:1942	recent years	1931:1942	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	1	18	theme	disease	181:187	arg1	syndrome					189:196	shell disease syndrome	175:196	shell disease syndrome	175:196	Cancer pagurus is highly susceptible to shell disease syndrome.
36342282	0	19	theme	Crustacean	108:117	arg1	pagurus					126:132	the Crustacean Cancer pagurus	104:132	the Crustacean Cancer pagurus	104:132	Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.
36342282	10	20	dep	Gammaproteobacteria	1328:1346	arg1	spp					1363:1365	spp	1363:1365	spp	1363:1365	The ability to degrade chitin was mainly found for Gammaproteobacteria and Aquimarina spp.
36342282	0	21	from	Diversity	75:83	arg1	Carapace					92:99	the Carapace	88:99	the Carapace of the Crustacean Cancer pagurus	88:132	Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.
36342282	17	22	theme	reduced	2499:2505	arg1	diversity					2517:2525	a reduced bacterial diversity	2497:2525	a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases	2497:2604	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	13	23	theme	community	1751:1759	arg1	analysis					1761:1768	bacterial community analysis	1741:1768	bacterial community analysis	1741:1768	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	0	24	theme	pagurus	126:132	arg1	Carapace					92:99	the Carapace	88:99	the Carapace of the Crustacean Cancer pagurus	88:132	Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.
36342282	6	25	theme	reduced	713:719	arg1	composition					753:763	a reduced and shifted microbial community composition	711:763	a reduced and shifted microbial community composition caused by the black spot disease	711:796	This indicates a reduced and shifted microbial community composition caused by the black spot disease.
36342282	3	26	theme	bacterial	297:305	arg1	communities					307:317	the bacterial communities	293:317	the bacterial communities of black spot affected and nonaffected areas of the carapace	293:378	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	12	27	theme	most	1588:1591	arg1	spp.					1604:1607	most Aquimarina spp.	1588:1607	most Aquimarina spp.	1588:1607	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	15	28	from	areas	2188:2192	arg1	pagurus					2157:2163	the widely distributed species Cancer pagurus	2119:2163	the widely distributed species Cancer pagurus in different North Sea areas	2119:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	15	28	from	areas	2188:2192	arg1	%					2114:2114	>60%	2111:2114	>60% of the widely distributed species Cancer pagurus in different North Sea areas	2111:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	15	29	theme	populations	2079:2089	arg1	proportions					2064:2074	Large proportions	2058:2074	Large proportions of populations	2058:2089	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	5	30	theme	different	667:675	arg1	areas					689:693	different nonaffected areas	667:693	different nonaffected areas	667:693	Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas.
36342282	3	31	theme	spot	328:331	arg1	areas					358:362	black spot affected and nonaffected areas	322:362	black spot affected and nonaffected areas of the carapace	322:378	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	16	32	theme	chitinolytic	2315:2326	arg1	degradation					2328:2338	microbial chitinolytic degradation	2305:2338	microbial chitinolytic degradation of the outer shell	2305:2357	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	2	33	theme	concomitant	230:240	arg1	changes					242:248	concomitant changes	230:248	concomitant changes in the epibacterial community	230:278	However, little is known about concomitant changes in the epibacterial community.
36342282	4	34	theme	affected	478:485	arg1	areas					487:491	affected areas	478:491	affected areas	478:491	Within each spot, bacterial communities of affected areas were less diverse compared to communities from nonaffected areas.
36342282	18	35	theme	increased	2729:2737	arg1	activity					2739:2746	increased activity	2729:2746	increased activity	2729:2746	Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease.
36342282	16	36	theme	outer	2347:2351	arg1	shell					2353:2357	the outer shell	2343:2357	the outer shell	2343:2357	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	5	37	theme	spots	593:597	arg1	Communities					559:569	Communities	559:569	Communities of different affected spots	559:597	Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas.
36342282	19	38	theme	deeper	2892:2897	arg1	insights					2899:2906	deeper insights	2892:2906	deeper insights into their physiological properties and thus the possible role within the microbiome	2892:2991	Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
36342282	3	39	theme	areas	358:362	arg1	communities					307:317	the bacterial communities	293:317	the bacterial communities of black spot affected and nonaffected areas of the carapace	293:378	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	11	40	theme	most	1500:1503	arg1	Alphaproteobacteria					1530:1548	many Alphaproteobacteria	1525:1548	many Alphaproteobacteria	1525:1548	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	11	40	theme	most	1500:1503	arg1	isolates					1505:1512	most isolates	1500:1512	most isolates	1500:1512	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	8	41	theme	spp.	1015:1018	arg1	increase					992:999	the increase	988:999	the increase of Aquimarina spp.	988:1018	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	17	42	theme	many	2586:2589	arg1	diseases					2597:2604	many other diseases	2586:2604	many other diseases	2586:2604	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	3	43	theme	16S	425:427	arg1	rRNA					429:432	16S rRNA	425:432	16S rRNA	425:432	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	13	44	theme	pagurus	1908:1914	arg1	carapace					1893:1900	the carapace	1889:1900	the carapace of C. pagurus	1889:1914	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	0	45	theme	Shell	0:4	arg1	Syndrome					14:21	Shell Disease Syndrome	0:21	Shell Disease Syndrome	0:21	Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.
36342282	8	46	theme	significant	1034:1044	arg1	role					1046:1049	a significant role	1032:1049	a significant role in shell disease syndrome	1032:1075	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	5	47	theme	different	574:582	arg1	spots					593:597	different affected spots	574:597	different affected spots	574:597	Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas.
36342282	14	48	theme	crustacean	2038:2047	arg1	species					2049:2055	several ecologically and economically important crustacean species	1990:2055	several ecologically and economically important crustacean species	1990:2055	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	2	49	from	changes	242:248	arg1	community					270:278	the epibacterial community	253:278	the epibacterial community	253:278	However, little is known about concomitant changes in the epibacterial community.
36342282	4	50	from	areas	552:556	arg1	communities					523:533	communities	523:533	communities from nonaffected areas	523:556	Within each spot, bacterial communities of affected areas were less diverse compared to communities from nonaffected areas.
36342282	11	51	theme	many	1525:1528	arg1	Alphaproteobacteria					1530:1548	many Alphaproteobacteria	1525:1548	many Alphaproteobacteria	1525:1548	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	11	52	theme	polysaccharide	1460:1473	arg1	chitin					1475:1480	the polysaccharide chitin	1456:1480	the polysaccharide chitin	1456:1480	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	3	53	theme	16S	406:408	arg1	rRNA					410:413	16S rRNA genes and 16S rRNA	406:432	rRNA	410:413	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	17	54	theme	shell	2436:2440	arg1	areas					2423:2427	healthy and diseased areas	2402:2427	healthy and diseased areas of the shell of C. pagurus	2402:2454	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	7	55	located	found	821:825	arg2	communities					809:819	Different communities	799:819	Different communities found in black spots	799:840	Different communities found in black spots likely indicate different stages of the disease.
36342282	7	55	located	found	821:825	arg1	spots					836:840	black spots	830:840	black spots	830:840	Different communities found in black spots likely indicate different stages of the disease.
36342282	18	56	theme	relative	2697:2704	arg1	abundances					2706:2715	high relative abundances	2692:2715	high relative abundances	2692:2715	Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease.
36342282	8	57	theme	disease	1060:1066	arg1	syndrome					1068:1075	shell disease syndrome	1054:1075	shell disease syndrome	1054:1075	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	6	58	theme	community	743:751	arg1	composition					753:763	a reduced and shifted microbial community composition	711:763	a reduced and shifted microbial community composition caused by the black spot disease	711:796	This indicates a reduced and shifted microbial community composition caused by the black spot disease.
36342282	13	59	from	community	1876:1884	arg1	carapace					1893:1900	the carapace	1889:1900	the carapace of C. pagurus	1889:1914	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	17	60	theme	bacterial	2507:2515	arg1	diversity					2517:2525	a reduced bacterial diversity	2497:2525	a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases	2497:2604	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	9	61	theme	diseased	1116:1123	arg1	areas					1137:1141	diseased and healthy areas	1116:1141	diseased and healthy areas	1116:1141	We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing.
36342282	15	62	theme	distributed	2130:2140	arg1	pagurus					2157:2163	the widely distributed species Cancer pagurus	2119:2163	the widely distributed species Cancer pagurus in different North Sea areas	2119:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	15	63	dep	%	2114:2114	arg1	e.g.					2105:2108	e.g.	2105:2108	e.g.	2105:2108	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	9	64	theme	healthy	1129:1135	arg1	areas					1137:1141	diseased and healthy areas	1116:1141	diseased and healthy areas	1116:1141	We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing.
36342282	13	65	theme	functional	1844:1853	arg1	community					1876:1884	a functional complex epibacterial community	1842:1884	a functional complex epibacterial community on the carapace of C. pagurus	1842:1914	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	7	66	theme	disease	882:888	arg1	stages					868:873	different stages	858:873	different stages of the disease	858:888	Different communities found in black spots likely indicate different stages of the disease.
36342282	17	67	theme	healthy	2402:2408	arg1	areas					2423:2427	healthy and diseased areas	2402:2427	healthy and diseased areas of the shell of C. pagurus	2402:2454	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	19	68	theme	possible	2957:2964	arg1	role					2966:2969	thus the possible role	2948:2969	thus the possible role within the microbiome	2948:2991	Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
36342282	15	69	theme	species	2142:2148	arg1	pagurus					2157:2163	the widely distributed species Cancer pagurus	2119:2163	the widely distributed species Cancer pagurus in different North Sea areas	2119:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	8	70	theme	families	972:979	arg1	families					972:979	the most abundant and active families	943:979	the most abundant and active families	943:979	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	8	70	theme	families	972:979	arg1	one					936:938	one	936:938	one	936:938	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	17	71	theme	diseased	2414:2421	arg1	areas					2423:2427	healthy and diseased areas	2402:2427	healthy and diseased areas of the shell of C. pagurus	2402:2454	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	16	72	theme	forms	2260:2264	arg1	development					2235:2245	the development	2231:2245	the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell	2231:2357	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	6	73	theme	spot	785:788	arg1	disease					790:796	the black spot disease	775:796	the black spot disease	775:796	This indicates a reduced and shifted microbial community composition caused by the black spot disease.
36342282	7	74	theme	different	858:866	arg1	stages					868:873	different stages	858:873	different stages of the disease	858:888	Different communities found in black spots likely indicate different stages of the disease.
36342282	14	75	theme	disease	1951:1957	arg1	syndrome					1959:1966	shell disease syndrome	1945:1966	shell disease syndrome	1945:1966	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	14	75	theme	disease	1951:1957	arg1	IMPORTANCE					1917:1926	IMPORTANCE	1917:1926	IMPORTANCE In recent years	1917:1942	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	1	76	theme	shell	175:179	arg1	syndrome					189:196	shell disease syndrome	175:196	shell disease syndrome	175:196	Cancer pagurus is highly susceptible to shell disease syndrome.
36342282	0	77	theme	Cancer	119:124	arg1	pagurus					126:132	the Crustacean Cancer pagurus	104:132	the Crustacean Cancer pagurus	104:132	Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.
36342282	15	78	theme	Cancer	2150:2155	arg1	pagurus					2157:2163	the widely distributed species Cancer pagurus	2119:2163	the widely distributed species Cancer pagurus in different North Sea areas	2119:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	7	79	theme	black	830:834	arg1	spots					836:840	black spots	830:840	black spots	830:840	Different communities found in black spots likely indicate different stages of the disease.
36342282	14	80	theme	recent	1931:1936	arg1	years					1938:1942	recent years	1931:1942	recent years	1931:1942	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	12	81	theme	antagonistic	1617:1628	arg1	properties					1630:1639	antagonistic properties	1617:1639	antagonistic properties	1617:1639	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	15	82	from	pagurus	2157:2163	arg1	areas					2188:2192	different North Sea areas	2168:2192	different North Sea areas	2168:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	13	83	theme	bacterial	1741:1749	arg1	analysis					1761:1768	bacterial community analysis	1741:1768	bacterial community analysis	1741:1768	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	15	84	theme	different	2168:2176	arg1	areas					2188:2192	different North Sea areas	2168:2192	different North Sea areas	2168:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	8	85	from	role	1046:1049	arg1	syndrome					1068:1075	shell disease syndrome	1054:1075	shell disease syndrome	1054:1075	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	13	86	theme	analysis	1761:1768	arg1	properties					1792:1801	the physiological properties	1774:1801	the physiological properties of the isolates	1774:1817	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	13	86	theme	analysis	1761:1768	arg1	combination					1726:1736	The combination	1722:1736	The combination of bacterial community analysis	1722:1768	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	9	87	theme	dominant	1229:1236	arg1	phyla					1238:1242	the dominant phyla	1225:1242	the dominant phyla detected by amplicon sequencing	1225:1274	We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing.
36342282	19	88	theme	nonaffected	2865:2875	arg1	spots					2877:2881	affected and nonaffected spots	2852:2881	spots	2877:2881	Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
36342282	12	89	theme	Aquimarina	1593:1602	arg1	spp.					1604:1607	most Aquimarina spp.	1588:1607	most Aquimarina spp.	1588:1607	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	5	90	theme	nonaffected	677:687	arg1	areas					689:693	different nonaffected areas	667:693	different nonaffected areas	667:693	Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas.
36342282	19	91	theme	affected	2852:2859	arg1	spots					2877:2881	affected and nonaffected spots	2852:2881	spots	2877:2881	Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
36342282	6	92	theme	shifted	725:731	arg1	composition					753:763	a reduced and shifted microbial community composition	711:763	a reduced and shifted microbial community composition caused by the black spot disease	711:796	This indicates a reduced and shifted microbial community composition caused by the black spot disease.
36342282	11	93	theme	chitin	1475:1480	arg1	chitin					1475:1480	the polysaccharide chitin	1456:1480	the polysaccharide chitin	1456:1480	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	11	93	theme	chitin	1475:1480	arg1	monomer					1445:1451	the monomer	1441:1451	the monomer	1441:1451	within the Flavobacteriia, while the ability to use N-acetylglucosamine, the monomer of the polysaccharide chitin, was observed for most isolates, including many Alphaproteobacteria.
36342282	0	94	theme	Epibacterial	62:73	arg1	Diversity					75:83	Epibacterial Diversity	62:83	Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus	62:132	Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.
36342282	9	95	theme	amplicon	1256:1263	arg1	sequencing					1265:1274	amplicon sequencing	1256:1274	amplicon sequencing	1256:1274	We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing.
36342282	15	96	theme	Sea	2184:2186	arg1	areas					2188:2192	different North Sea areas	2168:2192	different North Sea areas	2168:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	1	97	theme	Cancer	135:140	arg1	pagurus					142:148	Cancer pagurus	135:148	Cancer pagurus	135:148	Cancer pagurus is highly susceptible to shell disease syndrome.
36342282	12	98	theme	isolates	1568:1575	arg1	spp.					1604:1607	most Aquimarina spp.	1588:1607	most Aquimarina spp.	1588:1607	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	12	98	theme	isolates	1568:1575	arg1	One-third					1551:1559	One-third	1551:1559	One-third	1551:1559	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	12	98	theme	isolates	1568:1575	arg1	isolates					1568:1575	the isolates	1564:1575	the isolates	1564:1575	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	3	99	theme	affected	333:340	arg1	areas					358:362	black spot affected and nonaffected areas	322:362	black spot affected and nonaffected areas of the carapace	322:378	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	4	100	theme	areas	487:491	arg1	communities					463:473	bacterial communities	453:473	bacterial communities of affected areas	453:491	Within each spot, bacterial communities of affected areas were less diverse compared to communities from nonaffected areas.
36342282	16	101	theme	shell	2353:2357	arg1	degradation					2328:2338	microbial chitinolytic degradation	2305:2338	microbial chitinolytic degradation of the outer shell	2305:2357	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	8	102	theme	Aquimarina	1004:1013	arg1	spp.					1015:1018	Aquimarina spp.	1004:1018	Aquimarina spp.	1004:1018	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	3	103	theme	black	322:326	arg1	areas					358:362	black spot affected and nonaffected areas	322:362	black spot affected and nonaffected areas of the carapace	322:378	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	12	104	theme	bacterial	1699:1707	arg1	populations					1709:1719	the bacterial populations	1695:1719	the bacterial populations	1695:1719	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	4	105	theme	bacterial	453:461	arg1	communities					463:473	bacterial communities	453:473	bacterial communities of affected areas	453:491	Within each spot, bacterial communities of affected areas were less diverse compared to communities from nonaffected areas.
36342282	16	106	theme	shell	2269:2273	arg1	disease					2275:2281	shell disease	2269:2281	shell disease	2269:2281	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	18	107	theme	high	2692:2695	arg1	abundances					2706:2715	high relative abundances	2692:2715	high relative abundances	2692:2715	Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease.
36342282	12	108	theme	high	1655:1658	arg1	potential					1660:1668	a high potential	1653:1668	a high potential for interactions between the bacterial populations	1653:1719	One-third of the isolates, including most Aquimarina spp., showed antagonistic properties, indicating a high potential for interactions between the bacterial populations.
36342282	3	109	theme	nonaffected	346:356	arg1	areas					358:362	black spot affected and nonaffected areas	322:362	black spot affected and nonaffected areas of the carapace	322:378	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	17	110	theme	other	2591:2595	arg1	diseases					2597:2604	many other diseases	2586:2604	many other diseases	2586:2604	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	3	111	theme	rRNA	429:432	arg1	sequencing					392:401	amplicon sequencing	383:401	amplicon sequencing of 16S rRNA genes and 16S rRNA	383:432	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	0	112	theme	Disease	6:12	arg1	Syndrome					14:21	Shell Disease Syndrome	0:21	Shell Disease Syndrome	0:21	Shell Disease Syndrome Is Associated with Reduced and Shifted Epibacterial Diversity on the Carapace of the Crustacean Cancer pagurus.
36342282	18	113	from	participation	2767:2779	arg1	disease					2790:2796	shell disease	2784:2796	shell disease	2784:2796	Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease.
36342282	3	114	theme	amplicon	383:390	arg1	sequencing					392:401	amplicon sequencing	383:401	amplicon sequencing of 16S rRNA genes and 16S rRNA	383:432	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	4	115	theme	nonaffected	540:550	arg1	areas					552:556	nonaffected areas	540:556	nonaffected areas	540:556	Within each spot, bacterial communities of affected areas were less diverse compared to communities from nonaffected areas.
36342282	3	116	dep	rRNA	410:413	arg1	genes					415:419	genes	415:419	genes	415:419	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	13	117	theme	epibacterial	1863:1874	arg1	community					1876:1884	a functional complex epibacterial community	1842:1884	a functional complex epibacterial community on the carapace of C. pagurus	1842:1914	The combination of bacterial community analysis and the physiological properties of the isolates provided insights into a functional complex epibacterial community on the carapace of C. pagurus.
36342282	7	118	theme	Different	799:807	arg1	communities					809:819	Different communities	799:819	Different communities found in black spots	799:840	Different communities found in black spots likely indicate different stages of the disease.
36342282	8	119	theme	shell	1054:1058	arg1	syndrome					1068:1075	shell disease syndrome	1054:1075	shell disease syndrome	1054:1075	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	5	120	theme	affected	584:591	arg1	spots					593:597	different affected spots	574:597	different affected spots	574:597	Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas.
36342282	14	121	theme	important	2028:2036	arg1	species					2049:2055	several ecologically and economically important crustacean species	1990:2055	several ecologically and economically important crustacean species	1990:2055	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	3	122	theme	carapace	371:378	arg1	areas					358:362	black spot affected and nonaffected areas	322:362	black spot affected and nonaffected areas of the carapace	322:378	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	16	123	theme	microbial	2305:2313	arg1	degradation					2328:2338	microbial chitinolytic degradation	2305:2338	microbial chitinolytic degradation of the outer shell	2305:2357	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36342282	19	124	theme	bacterial	2819:2827	arg1	isolates					2829:2836	bacterial isolates	2819:2836	bacterial isolates obtained from affected and nonaffected spots	2819:2881	Characterization of bacterial isolates obtained from affected and nonaffected spots provided deeper insights into their physiological properties and thus the possible role within the microbiome.
36342282	15	125	theme	pagurus	2157:2163	arg1	pagurus					2157:2163	the widely distributed species Cancer pagurus	2119:2163	the widely distributed species Cancer pagurus in different North Sea areas	2119:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	15	125	theme	pagurus	2157:2163	arg1	%					2114:2114	>60%	2111:2114	>60% of the widely distributed species Cancer pagurus in different North Sea areas	2111:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	8	126	theme	affected	894:901	arg1	areas					903:907	affected areas	894:907	affected areas	894:907	In affected areas, Flavobacteriaceae rose to one of the most abundant and active families due to the increase of Aquimarina spp., suggesting a significant role in shell disease syndrome.
36342282	3	127	theme	rRNA	410:413	arg1	sequencing					392:401	amplicon sequencing	383:401	amplicon sequencing of 16S rRNA genes and 16S rRNA	383:432	We compared the bacterial communities of black spot affected and nonaffected areas of the carapace by amplicon sequencing of 16S rRNA genes and 16S rRNA.
36342282	15	128	theme	Large	2058:2062	arg1	proportions					2064:2074	Large proportions	2058:2074	Large proportions of populations	2058:2089	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	9	129	theme	bacterial	1093:1101	arg1	strains					1103:1109	75 bacterial strains	1090:1109	75 bacterial strains	1090:1109	We isolated 75 bacterial strains from diseased and healthy areas, which are primarily affiliated with Proteobacteria and Bacteroidetes, reflecting the dominant phyla detected by amplicon sequencing.
36342282	18	130	theme	strong	2760:2765	arg1	participation					2767:2779	strong participation	2760:2779	strong participation in shell disease	2760:2796	Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease.
36342282	18	131	theme	community	2624:2632	arg1	composition					2634:2644	the community composition	2620:2644	the community composition	2620:2644	Furthermore, the community composition dramatically changed with some taxa rising to high relative abundances and showing increased activity, indicating strong participation in shell disease.
36342282	14	132	theme	several	1990:1996	arg1	species					2049:2055	several ecologically and economically important crustacean species	1990:2055	several ecologically and economically important crustacean species	1990:2055	IMPORTANCE In recent years, shell disease syndrome has been detected for several ecologically and economically important crustacean species.
36342282	17	133	theme	pagurus	2448:2454	arg1	shell					2436:2440	the shell	2432:2440	the shell of C. pagurus	2432:2454	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	6	134	theme	microbial	733:741	arg1	composition					753:763	a reduced and shifted microbial community composition	711:763	a reduced and shifted microbial community composition caused by the black spot disease	711:796	This indicates a reduced and shifted microbial community composition caused by the black spot disease.
36342282	5	135	from	other	639:643	arg1	divergent					619:627	divergent	619:627	divergent	619:627	Communities of different affected spots were, however, more divergent from each other, compared to those of different nonaffected areas.
36342282	15	136	theme	North	2178:2182	arg1	areas					2188:2192	different North Sea areas	2168:2192	different North Sea areas	2168:2192	Large proportions of populations are affected, e.g., >60% of the widely distributed species Cancer pagurus in different North Sea areas.
36342282	17	137	theme	affected	2534:2541	arg1	areas					2543:2547	affected areas	2534:2547	affected areas	2534:2547	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	17	137	theme	affected	2534:2541	arg1	phenomenon					2552:2561	a phenomenon	2550:2561	a phenomenon co-occurring also with many other diseases	2550:2604	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	17	138	theme	bacterial	2377:2385	arg1	communities					2387:2397	the bacterial communities	2373:2397	the bacterial communities of healthy and diseased areas of the shell of C. pagurus	2373:2454	By comparing the bacterial communities of healthy and diseased areas of the shell of C. pagurus, we demonstrated that the disease causes a reduced bacterial diversity within affected areas, a phenomenon co-occurring also with many other diseases.
36342282	16	139	theme	significant	2211:2221	arg1	role					2223:2226	a significant role	2209:2226	a significant role	2209:2226	Bacteria play a significant role in the development of different forms of shell disease, all characterized by microbial chitinolytic degradation of the outer shell.
36244214	12	0	theme	brain	1807:1811	arg1	oxylipins					1813:1821	brain oxylipins	1807:1821	brain oxylipins	1807:1821	CONCLUSION A HSD substantially changes brain oxylipins in a distinctly sexually dimorphic manner.
36244214	8	1	dep	RESULTS	944:950	arg1	were					958:961	were	958:961	were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet	958:1097	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	14	2	theme	oxylipin	1983:1990	arg1	composition					1992:2002	brain oxylipin composition	1977:2002	brain oxylipin composition	1977:2002	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	10	3	theme	profiles	1517:1524	arg1	separation					1490:1499	the separation	1486:1499	the separation of the oxylipin profiles by diet in male mice	1486:1545	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	13	4	theme	links	1925:1929	arg1	terms					1891:1895	terms	1891:1895	terms of potential mechanisms and links to metabolic disease	1891:1950	Results are discussed in terms of potential mechanisms and links to metabolic disease.
36244214	10	5	from	separation	1490:1499	arg1	influential					1471:1481	influential	1471:1481	influential	1471:1481	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	2	6	theme	dementia	286:293	arg1	progression					271:281	progression	271:281	progression	271:281	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	2	6	theme	dementia	286:293	arg1	onset					261:265	onset	261:265	onset	261:265	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	13	7	theme	metabolic	1934:1942	arg1	disease					1944:1950	metabolic disease	1934:1950	metabolic disease	1934:1950	Results are discussed in terms of potential mechanisms and links to metabolic disease.
36244214	8	8	theme	fasting	1046:1052	arg1	levels					1068:1073	fasting serum insulin levels	1046:1073	fasting serum insulin levels	1046:1073	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	14	9	theme	diet	1961:1964	arg1	effects					1966:1972	Sex and diet effects	1953:1972	Sex and diet effects on brain oxylipin composition	1953:2002	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	10	10	theme	male	1537:1540	arg1	mice					1542:1545	male mice	1537:1545	male mice	1537:1545	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	8	11	theme	insulin	1060:1066	arg1	levels					1068:1073	fasting serum insulin levels	1046:1073	fasting serum insulin levels	1046:1073	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	8	12	from	mice	1079:1082	arg1	diet					1094:1097	either diet	1087:1097	either diet	1087:1097	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	14	13	theme	Sex	1953:1955	arg1	effects					1966:1972	Sex and diet effects	1953:1972	Sex and diet effects on brain oxylipin composition	1953:2002	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	10	14	from	diet	1529:1532	arg1	mice					1542:1545	male mice	1537:1545	male mice	1537:1545	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	5	15	theme	tandem	736:741	arg1	spectrometry					748:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	13	16	theme	potential	1900:1908	arg1	mechanisms					1910:1919	potential mechanisms	1900:1919	potential mechanisms	1900:1919	Results are discussed in terms of potential mechanisms and links to metabolic disease.
36244214	3	17	theme	dietary	310:316	arg1	interventions					318:330	most dietary interventions	305:330	most dietary interventions	305:330	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	12	18	theme	dimorphic	1848:1856	arg1	manner					1858:1863	a distinctly sexually dimorphic manner	1826:1863	a distinctly sexually dimorphic manner	1826:1863	CONCLUSION A HSD substantially changes brain oxylipins in a distinctly sexually dimorphic manner.
36244214	9	19	theme	lipoxygenase	1300:1311	arg1	enzymes					1313:1319	lipoxygenase enzymes	1300:1319	lipoxygenase enzymes	1300:1319	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	20	from	reduction	1336:1344	arg1	oxylipins					1355:1363	eight oxylipins	1349:1363	eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females	1349:1431	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	20	from	reduction	1336:1344	arg1	%					1279:1279	16%	1277:1279	16%	1277:1279	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	20	from	reduction	1336:1344	arg1	oxylipins					1222:1230	three oxylipins	1216:1230	three oxylipins (by 47-61%)	1216:1242	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	20	from	reduction	1336:1344	arg1	oxylipin					1267:1274	one oxylipin	1263:1274	one oxylipin (16%)	1263:1280	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	20	from	reduction	1336:1344	arg1	males					1324:1328	males	1324:1328	males	1324:1328	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	4	21	theme	age	623:625	arg1	weeks					614:618	20 weeks	611:618	20 weeks of age	611:625	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	7	22	theme	sex	927:929	arg1	differences					931:941	sex differences	927:941	sex differences	927:941	We first assessed whether there were any effects of the diet on the brain oxylipin profile, then assessed for sex differences.
36244214	9	23	theme	oxylipin	1126:1133	arg1	profile					1135:1141	the brain oxylipin profile	1116:1141	the brain oxylipin profile in both sexes in distinctly different patterns	1116:1188	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	7	24	theme	brain	885:889	arg1	profile					900:906	the brain oxylipin profile	881:906	the brain oxylipin profile	881:906	We first assessed whether there were any effects of the diet on the brain oxylipin profile, then assessed for sex differences.
36244214	1	25	theme	biological	122:131	arg1	processes					133:141	many biological processes	117:141	many biological processes	117:141	BACKGROUND Oxylipins have been implicated in many biological processes and diseases.
36244214	1	26	theme	many	117:120	arg1	processes					133:141	many biological processes	117:141	many biological processes	117:141	BACKGROUND Oxylipins have been implicated in many biological processes and diseases.
36244214	7	27	from	effects	858:864	arg1	profile					900:906	the brain oxylipin profile	881:906	the brain oxylipin profile	881:906	We first assessed whether there were any effects of the diet on the brain oxylipin profile, then assessed for sex differences.
36244214	5	28	theme	free	646:649	arg1	oxylipins					651:659	53 free oxylipins	643:659	53 free oxylipins	643:659	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	5	29	theme	liquid	714:719	arg1	spectrometry					748:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	8	30	from	differences	966:976	arg1	glucose					995:1001	fasting serum glucose	981:1001	fasting serum glucose	981:1001	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	9	31	dep	reduction	1203:1211	arg1	downstream					1286:1295	downstream	1286:1295	downstream	1286:1295	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	3	32	theme	fats	370:373	arg1	modulation					348:357	modulation	348:357	modulation of dietary fats	348:373	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	10	33	from	influential	1585:1595	arg1	separation					1604:1613	the separation	1600:1613	the separation by diet in female mice	1600:1636	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	4	34	theme	Male	460:463	arg1	mice					485:488	Male and female C57BL/6J mice	460:488	METHODS Male and female C57BL/6J mice	452:488	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	14	35	theme	neuroinflammatory	2053:2069	arg1	diseases					2071:2078	neuroinflammatory diseases	2053:2078	neuroinflammatory diseases	2053:2078	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	3	36	theme	oxylipin	423:430	arg1	profile					432:438	the brain oxylipin profile	413:438	the brain oxylipin profile	413:438	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	9	37	from	reduction	1203:1211	arg1	oxylipins					1355:1363	eight oxylipins	1349:1363	eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females	1349:1431	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	37	from	reduction	1203:1211	arg1	%					1279:1279	16%	1277:1279	16%	1277:1279	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	37	from	reduction	1203:1211	arg1	oxylipins					1222:1230	three oxylipins	1216:1230	three oxylipins (by 47-61%)	1216:1242	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	37	from	reduction	1203:1211	arg1	oxylipin					1267:1274	one oxylipin	1263:1274	one oxylipin (16%)	1263:1280	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	37	from	reduction	1203:1211	arg1	males					1324:1328	males	1324:1328	males	1324:1328	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	11	38	dep	Oxylipins	1639:1647	arg1	11-HETE					1689:1695	11-HETE	1689:1695	11-HETE	1689:1695	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	11	38	dep	Oxylipins	1639:1647	arg1	acid					1676:1679	9‑hydroxy-eicosatetraenoic acid	1649:1679	9‑hydroxy-eicosatetraenoic acid (HETE)	1649:1686	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	11	38	dep	Oxylipins	1639:1647	arg1	HETE					1682:1685	HETE	1682:1685	HETE	1682:1685	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	11	38	dep	Oxylipins	1639:1647	arg1	Oxylipins					1639:1647	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE	1639:1708	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE	1639:1708	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	11	38	dep	Oxylipins	1639:1647	arg1	15-HETE					1702:1708	15-HETE	1702:1708	15-HETE	1702:1708	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	9	39	from	activity	1413:1420	arg1	females					1425:1431	females	1425:1431	females	1425:1431	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	12	40	theme	A	1779:1779	arg1	HSD					1781:1783	CONCLUSION A HSD	1768:1783	CONCLUSION A HSD	1768:1783	CONCLUSION A HSD substantially changes brain oxylipins in a distinctly sexually dimorphic manner.
36244214	4	41	theme	female	469:474	arg1	mice					485:488	Male and female C57BL/6J mice	460:488	METHODS Male and female C57BL/6J mice	452:488	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	5	42	theme	ultra-high	691:700	arg1	spectrometry					748:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	0	43	theme	sucrose	7:13	arg1	diet					15:18	A high sucrose diet	0:18	A high sucrose diet	0:18	A high sucrose diet modifies brain oxylipins in a sex-dependent manner.
36244214	3	44	theme	diet	405:408	arg1	unknown					443:449	unknown	443:449	unknown	443:449	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	3	44	theme	diet	405:408	arg1	impact					380:385	the impact	376:385	the impact of a high sucrose diet on the brain oxylipin profile	376:438	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	2	45	theme	cerebral	174:181	arg1	homeostasis					189:199	cerebral lipid homeostasis	174:199	cerebral lipid homeostasis	174:199	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	10	46	dep	influential	1471:1481	arg1	whereas					1548:1554	whereas	1548:1554	whereas	1548:1554	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	14	47	theme	future	2016:2021	arg1	dementia					2089:2096	dementia	2089:2096	dementia	2089:2096	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	14	47	theme	future	2016:2021	arg1	targets					2023:2029	future targets	2016:2029	future targets	2016:2029	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	4	48	from	diet	514:517	arg1	comparison					533:542	comparison	533:542	comparison to a control low sucrose diet (LSD, 12%)	533:583	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	8	49	theme	fasting	981:987	arg1	glucose					995:1001	fasting serum glucose	981:1001	fasting serum glucose	981:1001	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	9	50	theme	downstream	1384:1393	arg1	oxylipins					1355:1363	eight oxylipins	1349:1363	eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females	1349:1431	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	3	51	theme	high	392:395	arg1	diet					405:408	a high sucrose diet	390:408	a high sucrose diet	390:408	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	4	52	theme	sucrose	506:512	arg1	diet					514:517	a high sucrose diet	499:517	a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%)	499:583	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	10	53	theme	female	1626:1631	arg1	mice					1633:1636	female mice	1626:1636	female mice	1626:1636	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	10	54	theme	oxylipin	1508:1515	arg1	profiles					1517:1524	the oxylipin profiles	1504:1524	the oxylipin profiles	1504:1524	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	9	55	theme	cyclooxygenase	1398:1411	arg1	activity					1413:1420	cyclooxygenase activity	1398:1420	cyclooxygenase activity in females	1398:1431	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	2	56	theme	altered	205:211	arg1	metabolites					219:229	altered lipid metabolites	205:229	altered lipid metabolites	205:229	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	8	57	from	or	1040:1041	arg1	levels					1068:1073	fasting serum insulin levels	1046:1073	fasting serum insulin levels	1046:1073	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	10	58	from	influential	1471:1481	arg1	separation					1490:1499	the separation	1486:1499	the separation of the oxylipin profiles by diet in male mice	1486:1545	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	2	59	theme	homeostasis	189:199	arg1	Dysregulation					157:169	Dysregulation	157:169	Dysregulation of cerebral lipid homeostasis	157:199	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	2	59	theme	homeostasis	189:199	arg1	metabolites					219:229	altered lipid metabolites	205:229	altered lipid metabolites	205:229	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	9	60	from	profile	1135:1141	arg1	sexes					1151:1155	both sexes	1146:1155	both sexes	1146:1155	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	60	from	profile	1135:1141	arg1	patterns					1181:1188	distinctly different patterns	1160:1188	distinctly different patterns	1160:1188	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	4	61	theme	high	501:504	arg1	diet					514:517	a high sucrose diet	499:517	a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%)	499:583	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	11	62	theme	9‑hydroxy-eicosatetraenoic	1649:1674	arg1	acid					1676:1679	9‑hydroxy-eicosatetraenoic acid	1649:1679	9‑hydroxy-eicosatetraenoic acid (HETE)	1649:1686	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	11	62	theme	9‑hydroxy-eicosatetraenoic	1649:1674	arg1	Oxylipins					1639:1647	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE	1639:1708	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE	1639:1708	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	11	62	theme	9‑hydroxy-eicosatetraenoic	1649:1674	arg1	HETE					1682:1685	HETE	1682:1685	HETE	1682:1685	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	4	63	dep	METHODS	452:458	arg1	mice					485:488	Male and female C57BL/6J mice	460:488	METHODS Male and female C57BL/6J mice	452:488	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	14	64	theme	brain	1977:1981	arg1	composition					1992:2002	brain oxylipin composition	1977:2002	brain oxylipin composition	1977:2002	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	8	65	theme	HSD	1036:1038	arg1	or					1040:1041	a HSD or	1034:1041	a HSD or in fasting serum insulin levels for mice on either diet	1034:1097	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	12	66	theme	CONCLUSION	1768:1777	arg1	HSD					1781:1783	CONCLUSION A HSD	1768:1783	CONCLUSION A HSD	1768:1783	CONCLUSION A HSD substantially changes brain oxylipins in a distinctly sexually dimorphic manner.
36244214	8	67	theme	serum	1054:1058	arg1	levels					1068:1073	fasting serum insulin levels	1046:1073	fasting serum insulin levels	1046:1073	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	2	68	dep	onset	261:265	arg1	the					257:259	the	257:259	the	257:259	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	4	69	dep	diet	569:572	arg1	LSD					575:577	LSD	575:577	LSD	575:577	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	4	69	dep	diet	569:572	arg1	%					582:582	12%	580:582	12%	580:582	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	5	70	theme	chromatography	721:734	arg1	spectrometry					748:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	5	71	theme	mass	743:746	arg1	spectrometry					748:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	13	72	theme	mechanisms	1910:1919	arg1	terms					1891:1895	terms	1891:1895	terms of potential mechanisms and links to metabolic disease	1891:1950	Results are discussed in terms of potential mechanisms and links to metabolic disease.
36244214	9	73	theme	brain	1120:1124	arg1	profile					1135:1141	the brain oxylipin profile	1116:1141	the brain oxylipin profile in both sexes in distinctly different patterns	1116:1188	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	74	theme	enzymes	1313:1319	arg1	reduction					1203:1211	a reduction	1201:1211	a reduction in three oxylipins (by 47-61%)	1201:1242	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	74	theme	enzymes	1313:1319	arg1	increase					1251:1258	an increase	1248:1258	an increase in one oxylipin (16%)	1248:1280	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	74	theme	enzymes	1313:1319	arg1	reduction					1336:1344	a reduction	1334:1344	a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females	1334:1431	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	3	75	theme	most	305:308	arg1	interventions					318:330	most dietary interventions	305:330	most dietary interventions	305:330	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	0	76	theme	sex-dependent	50:62	arg1	manner					64:69	a sex-dependent manner	48:69	a sex-dependent manner	48:69	A high sucrose diet modifies brain oxylipins in a sex-dependent manner.
36244214	11	77	theme	females	1739:1745	arg1	brains					1729:1734	the brains	1725:1734	the brains of females	1725:1745	Oxylipins 9‑hydroxy-eicosatetraenoic acid (HETE), 11-HETE, and 15-HETE were higher in the brains of females, regardless of diet.
36244214	7	78	theme	oxylipin	891:898	arg1	profile					900:906	the brain oxylipin profile	881:906	the brain oxylipin profile	881:906	We first assessed whether there were any effects of the diet on the brain oxylipin profile, then assessed for sex differences.
36244214	5	79	theme	oxylipins	651:659	arg1	profile					632:638	The profile	628:638	The profile of 53 free oxylipins	628:659	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	4	80	dep	diet	514:517	arg1	HSD					520:522	HSD	520:522	HSD	520:522	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	4	80	dep	diet	514:517	arg1	%					527:527	34%	525:527	34%	525:527	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	14	81	from	effects	1966:1972	arg1	composition					1992:2002	brain oxylipin composition	1977:2002	brain oxylipin composition	1977:2002	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	7	82	theme	diet	873:876	arg1	effects					858:864	any effects	854:864	any effects of the diet on the brain oxylipin profile	854:906	We first assessed whether there were any effects of the diet on the brain oxylipin profile, then assessed for sex differences.
36244214	14	83	theme	diseases	2071:2078	arg1	management					2039:2048	the management	2035:2048	the management of neuroinflammatory diseases	2035:2078	Sex and diet effects on brain oxylipin composition may provide future targets for the management of neuroinflammatory diseases, such as dementia.
36244214	9	84	theme	different	1171:1179	arg1	patterns					1181:1188	distinctly different patterns	1160:1188	distinctly different patterns	1160:1188	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	3	85	theme	dietary	362:368	arg1	fats					370:373	dietary fats	362:373	dietary fats	362:373	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	4	86	theme	C57BL/6J	476:483	arg1	mice					485:488	Male and female C57BL/6J mice	460:488	METHODS Male and female C57BL/6J mice	452:488	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	0	87	theme	high	2:5	arg1	diet					15:18	A high sucrose diet	0:18	A high sucrose diet	0:18	A high sucrose diet modifies brain oxylipins in a sex-dependent manner.
36244214	1	88	theme	BACKGROUND	72:81	arg1	Oxylipins					83:91	BACKGROUND Oxylipins	72:91	BACKGROUND Oxylipins	72:91	BACKGROUND Oxylipins have been implicated in many biological processes and diseases.
36244214	3	89	from	impact	380:385	arg1	profile					432:438	the brain oxylipin profile	413:438	the brain oxylipin profile	413:438	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	3	90	theme	brain	417:421	arg1	profile					432:438	the brain oxylipin profile	413:438	the brain oxylipin profile	413:438	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	5	91	theme	performance	702:712	arg1	spectrometry					748:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	ultra-high performance liquid chromatography tandem mass spectrometry	691:759	The profile of 53 free oxylipins was then measured in brain by ultra-high performance liquid chromatography tandem mass spectrometry.
36244214	9	92	from	increase	1251:1258	arg1	oxylipins					1355:1363	eight oxylipins	1349:1363	eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females	1349:1431	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	92	from	increase	1251:1258	arg1	%					1279:1279	16%	1277:1279	16%	1277:1279	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	92	from	increase	1251:1258	arg1	oxylipins					1222:1230	three oxylipins	1216:1230	three oxylipins (by 47-61%)	1216:1242	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	92	from	increase	1251:1258	arg1	oxylipin					1267:1274	one oxylipin	1263:1274	one oxylipin (16%)	1263:1280	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	9	92	from	increase	1251:1258	arg1	males					1324:1328	males	1324:1328	males	1324:1328	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	10	93	from	separation	1604:1613	arg1	mice					1633:1636	female mice	1626:1636	female mice	1626:1636	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	10	93	from	separation	1604:1613	arg1	influential					1585:1595	influential	1585:1595	influential	1585:1595	9-oxo-ODE and 6-trans-LTB4 were most influential in the separation of the oxylipin profiles by diet in male mice, whereas 5-HEPE and 12-HEPE were most influential in the separation by diet in female mice.
36244214	2	94	theme	lipid	183:187	arg1	homeostasis					189:199	cerebral lipid homeostasis	174:199	cerebral lipid homeostasis	174:199	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	0	95	theme	brain	29:33	arg1	oxylipins					35:43	brain oxylipins	29:43	brain oxylipins	29:43	A high sucrose diet modifies brain oxylipins in a sex-dependent manner.
36244214	8	96	theme	serum	989:993	arg1	glucose					995:1001	fasting serum glucose	981:1001	fasting serum glucose	981:1001	RESULTS There were no differences in fasting serum glucose between the sexes for mice fed a HSD or in fasting serum insulin levels for mice on either diet.
36244214	6	97	dep	Serum	762:766	arg1	insulin					780:786	insulin	780:786	insulin	780:786	Serum glucose and insulin were measured enzymatically.
36244214	6	97	dep	Serum	762:766	arg1	glucose					768:774	glucose	768:774	glucose	768:774	Serum glucose and insulin were measured enzymatically.
36244214	3	98	theme	sucrose	397:403	arg1	diet					405:408	a high sucrose diet	390:408	a high sucrose diet	390:408	Although most dietary interventions have focused on modulation of dietary fats, the impact of a high sucrose diet on the brain oxylipin profile is unknown.
36244214	2	99	theme	lipid	213:217	arg1	metabolites					219:229	altered lipid metabolites	205:229	altered lipid metabolites	205:229	Dysregulation of cerebral lipid homeostasis and altered lipid metabolites have been associated with the onset and progression of dementia.
36244214	9	100	theme	activity	1413:1420	arg1	downstream					1384:1393	downstream	1384:1393	downstream	1384:1393	The HSD altered the brain oxylipin profile in both sexes in distinctly different patterns: there was a reduction in three oxylipins (by 47-61%) and an increase in one oxylipin (16%) all downstream of lipoxygenase enzymes in males and a reduction in eight oxylipins (by 14-94%) mostly downstream of cyclooxygenase activity in females.
36244214	4	101	theme	control	549:555	arg1	diet					569:572	a control low sucrose diet	547:572	a control low sucrose diet (LSD, 12%)	547:583	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	4	102	theme	sucrose	561:567	arg1	diet					569:572	a control low sucrose diet	547:572	a control low sucrose diet (LSD, 12%)	547:583	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
36244214	4	103	theme	low	557:559	arg1	diet					569:572	a control low sucrose diet	547:572	a control low sucrose diet (LSD, 12%)	547:583	METHODS Male and female C57BL/6J mice were fed a high sucrose diet (HSD, 34%) in comparison to a control low sucrose diet (LSD, 12%) for 12 weeks beginning at 20 weeks of age.
35802400	0	0	theme	optical	77:83	arg1	tomography					95:104	optical coherence tomography	77:104	optical coherence tomography	77:104	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	4	1	theme	acoustical	864:873	arg1	reflector					875:883	an acoustical reflector	861:883	an acoustical reflector	861:883	The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS.
35802400	2	2	theme	thicker	541:547	arg1	≥4 mm					558:562	≥4 mm	558:562	≥4 mm	558:562	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	2	theme	thicker	541:547	arg1	lesions					549:555	thicker lesions	541:555	thicker lesions (≥4 mm)	541:563	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	6	3	theme	mouse	1297:1301	arg1	models					1303:1308	mouse models	1297:1308	mouse models with ∼4.3 mm thick melanoma	1297:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	1	4	theme	single-head	149:159	arg1	system					180:185	a new single-head multimodal optical system	143:185	a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement	143:396	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	4	5	theme	same	927:930	arg1	position					932:939	the same position	923:939	the same position as OCT and RS	923:953	The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS.
35802400	1	6	theme	ultrasound	246:255	arg1	tomography					262:271	18 MHz ultrasound (US) tomography	239:271	18 MHz ultrasound (US) tomography	239:271	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	6	7	theme	depth	1217:1221	arg1	capabilities					1223:1234	the resolution and depth capabilities	1198:1234	capabilities	1223:1234	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	1	8	theme	fast	315:318	arg1	diagnostics					357:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	9	dep	ultrasound	246:255	arg1	US					258:259	US	258:259	US	258:259	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	10	theme	multimodal	161:170	arg1	system					180:185	a new single-head multimodal optical system	143:185	a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement	143:396	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	5	11	theme	flexible	1106:1113	arg1	membrane					1115:1122	a flexible membrane	1104:1122	a flexible membrane	1104:1122	The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane.
35802400	4	12	theme	other	831:835	arg1	side					837:840	the other side	827:840	the other side	827:840	The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS.
35802400	3	13	theme	optical	762:768	arg1	setup					770:774	the same scanning and optical setup	740:774	setup	770:774	The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements.
35802400	6	14	theme	resolution	1202:1211	arg1	capabilities					1223:1234	the resolution and depth capabilities	1198:1234	capabilities	1223:1234	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	0	15	theme	coherence	85:93	arg1	tomography					95:104	optical coherence tomography	77:104	optical coherence tomography	77:104	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	2	16	theme	penetration	520:530	arg1	depth					532:536	the penetration depth	516:536	the penetration depth of thicker lesions (≥4 mm)	516:563	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	3	17	theme	co-localized	790:801	arg1	measurements					803:814	co-localized measurements	790:814	co-localized measurements	790:814	The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements.
35802400	0	18	theme	Raman	110:114	arg1	spectroscopy					116:127	Raman spectroscopy	110:127	Raman spectroscopy	110:127	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	5	19	theme	B-mode	963:968	arg1	scans					970:974	The US B-mode scans	956:974	The US B-mode scans	956:974	The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane.
35802400	2	20	from	spot	631:634	arg1	composition					599:609	the chemical composition	586:609	the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma	586:714	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	6	21	theme	∼4.3 mm	1315:1321	arg1	melanoma					1329:1336	∼4.3 mm thick melanoma	1315:1336	∼4.3 mm thick melanoma	1315:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	1	22	theme	optical	172:178	arg1	system					180:185	a new single-head multimodal optical system	143:185	a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement	143:396	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	2	23	theme	lesions	468:474	arg1	information					440:450	structural and depth information	419:450	structural and depth information of smaller skin lesions (<1 mm)	419:482	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	4	24	theme	B-mode	900:905	arg1	measurements					907:918	B-mode measurements	900:918	B-mode measurements on the same position as OCT and RS	900:953	The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS.
35802400	4	25	from	measurements	907:918	arg1	position					932:939	the same position	923:939	the same position as OCT and RS	923:953	The US on the other side is integrated with an acoustical reflector, which enables B-mode measurements on the same position as OCT and RS.
35802400	2	26	theme	chemical	590:597	arg1	composition					599:609	the chemical composition	586:609	the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma	586:714	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	0	27	theme	Multimodal	0:9	arg1	system					11:16	Multimodal system	0:16	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.	0:128	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	1	28	dep	fast	315:318	arg1	min					324:326	<2 min	321:326	<2 min	321:326	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	2	29	theme	lesions	549:555	arg1	depth					532:536	the penetration depth	516:536	the penetration depth of thicker lesions (≥4 mm)	516:563	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	30	theme	benign	686:691	arg1	melanoma					707:714	benign and malignant melanoma	686:714	benign and malignant melanoma	686:714	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	0	31	theme	optical	22:28	arg1	biopsy					30:35	optical biopsy	22:35	optical biopsy of melanoma	22:47	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	5	32	theme	US	1036:1037	arg1	transducer					1039:1048	the US transducer	1032:1048	the US transducer	1032:1048	The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane.
35802400	1	33	theme	optical	203:209	arg1	OCT					233:235	OCT	233:235	OCT	233:235	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	33	theme	optical	203:209	arg1	tomography					221:230	optical coherence tomography	203:230	optical coherence tomography (OCT)	203:236	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	34	theme	Raman	277:281	arg1	RS					297:298	RS	297:298	RS	297:298	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	34	theme	Raman	277:281	arg1	spectroscopy					283:294	Raman spectroscopy	277:294	Raman spectroscopy (RS)	277:299	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	35	theme	noninvasive	333:343	arg1	diagnostics					357:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	36	theme	coherence	211:219	arg1	OCT					233:235	OCT	233:235	OCT	233:235	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	36	theme	coherence	211:219	arg1	tomography					221:230	optical coherence tomography	203:230	optical coherence tomography (OCT)	203:236	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	2	37	theme	depth	434:438	arg1	information					440:450	structural and depth information	419:450	structural and depth information of smaller skin lesions (<1 mm)	419:482	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	6	38	with	models	1303:1308	arg1	melanoma					1329:1336	∼4.3 mm thick melanoma	1315:1336	∼4.3 mm thick melanoma	1315:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	6	39	theme	agar	1174:1177	arg1	phantoms					1179:1186	agar phantoms	1174:1186	agar phantoms	1174:1186	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	1	40	theme	skin	345:348	arg1	diagnostics					357:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	5	41	dep	scans	970:974	arg1	US					960:961	US	960:961	US	960:961	The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane.
35802400	1	42	theme	cancer	350:355	arg1	diagnostics					357:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	fast (<2 min) and noninvasive skin cancer diagnostics	315:367	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	6	43	theme	preliminary	1261:1271	arg1	measurements					1281:1292	preliminary ex vivo measurements	1261:1292	preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma	1261:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	2	44	theme	chosen	624:629	arg1	≤300 μm					637:643	≤300 μm	637:643	≤300 μm	637:643	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	44	theme	chosen	624:629	arg1	spot					631:634	a small chosen spot	616:634	a small chosen spot (≤300 μm)	616:644	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	45	theme	structural	419:428	arg1	information					440:450	structural and depth information	419:450	structural and depth information of smaller skin lesions (<1 mm)	419:482	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	6	46	from	results	1140:1146	arg1	liquid					1163:1168	liquid	1163:1168	liquid	1163:1168	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	6	46	from	results	1140:1146	arg1	phantoms					1179:1186	agar phantoms	1174:1186	agar phantoms	1174:1186	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	2	47	theme	smaller	455:461	arg1	<1 mm					477:481	<1 mm	477:481	<1 mm	477:481	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	47	theme	smaller	455:461	arg1	lesions					468:474	smaller skin lesions	455:474	smaller skin lesions (<1 mm)	455:482	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	48	theme	small	618:622	arg1	≤300 μm					637:643	≤300 μm	637:643	≤300 μm	637:643	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	48	theme	small	618:622	arg1	spot					631:634	a small chosen spot	616:634	a small chosen spot (≤300 μm)	616:644	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	6	49	theme	ex	1273:1274	arg1	measurements					1281:1292	preliminary ex vivo measurements	1261:1292	preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma	1261:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	0	50	theme	melanoma	40:47	arg1	biopsy					30:35	optical biopsy	22:35	optical biopsy of melanoma	22:47	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	3	51	dep	RS	721:722	arg1	The					717:719	The	717:719	The	717:719	The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements.
35802400	0	52	with	system	11:16	arg1	tomography					95:104	optical coherence tomography	77:104	optical coherence tomography	77:104	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	0	52	with	system	11:16	arg1	spectroscopy					116:127	Raman spectroscopy	110:127	Raman spectroscopy	110:127	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	0	52	with	system	11:16	arg1	ultrasound					65:74	integrated ultrasound	54:74	integrated ultrasound	54:74	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	3	53	theme	same	744:747	arg1	scanning					749:756	the same scanning and optical setup	740:774	scanning	749:756	The RS and OCT utilize the same scanning and optical setup, allowing for co-localized measurements.
35802400	1	54	theme	lesion	373:378	arg1	measurement					386:396	lesion depth measurement	373:396	lesion depth measurement	373:396	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	6	55	theme	thick	1323:1327	arg1	melanoma					1329:1336	∼4.3 mm thick melanoma	1315:1336	∼4.3 mm thick melanoma	1315:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	6	56	dep	ex	1273:1274	arg1	vivo					1276:1279	vivo	1276:1279	vivo	1276:1279	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	1	57	theme	depth	380:384	arg1	measurement					386:396	lesion depth measurement	373:396	lesion depth measurement	373:396	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	6	58	from	measurements	1281:1292	arg1	models					1303:1308	mouse models	1297:1308	mouse models with ∼4.3 mm thick melanoma	1297:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	2	59	theme	malignant	697:705	arg1	melanoma					707:714	benign and malignant melanoma	686:714	benign and malignant melanoma	686:714	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	60	theme	skin	463:466	arg1	<1 mm					477:481	<1 mm	477:481	<1 mm	477:481	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	2	60	theme	skin	463:466	arg1	lesions					468:474	smaller skin lesions	455:474	smaller skin lesions (<1 mm)	455:482	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	5	61	with	adapter	1091:1097	arg1	membrane					1115:1122	a flexible membrane	1104:1122	a flexible membrane	1104:1122	The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane.
35802400	6	62	theme	setup	1243:1247	arg1	capabilities					1223:1234	the resolution and depth capabilities	1198:1234	capabilities	1223:1234	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	6	62	theme	setup	1243:1247	arg1	measurements					1281:1292	preliminary ex vivo measurements	1261:1292	preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma	1261:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	5	63	theme	developed	1081:1089	arg1	adapter					1091:1097	the developed adapter	1077:1097	the developed adapter with a flexible membrane	1077:1122	The US B-mode scans can be translated across the sample by laterally moving the US transducer, which is made possible by the developed adapter with a flexible membrane.
35802400	0	64	theme	integrated	54:63	arg1	ultrasound					65:74	integrated ultrasound	54:74	integrated ultrasound	54:74	Multimodal system for optical biopsy of melanoma with integrated ultrasound, optical coherence tomography and Raman spectroscopy.
35802400	6	65	from	capabilities	1223:1234	arg1	models					1303:1308	mouse models	1297:1308	mouse models with ∼4.3 mm thick melanoma	1297:1336	We present the results on custom-made liquid and agar phantoms that show the resolution and depth capabilities of the setup, as well as preliminary ex vivo measurements on mouse models with ∼4.3 mm thick melanoma.
35802400	2	66	used	used	658:661	arg2	composition					599:609	the chemical composition	586:609	the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma	586:714	The OCT can deliver structural and depth information of smaller skin lesions (<1 mm), while the US allows to measure the penetration depth of thicker lesions (≥4 mm), and the RS analyzes the chemical composition from a small chosen spot (≤300 μm) that can be used to distinguish between benign and malignant melanoma.
35802400	1	67	theme	new	145:147	arg1	system					180:185	a new single-head multimodal optical system	143:185	a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement	143:396	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35802400	1	68	theme	18 MHz	239:244	arg1	ultrasound					246:255	18 MHz ultrasound	239:255	18 MHz ultrasound (US) tomography	239:271	We introduce a new single-head multimodal optical system that integrates optical coherence tomography (OCT), 18 MHz ultrasound (US) tomography and Raman spectroscopy (RS), allowing for fast (<2 min) and noninvasive skin cancer diagnostics and lesion depth measurement.
35297441	2	0	theme	signal	621:626	arg1	proteins					644:651	signal pathway-related proteins	621:651	signal pathway-related proteins	621:651	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	4	1	theme	signaling	1081:1089	arg1	pathway					1091:1097	the NF-κB signaling pathway	1071:1097	the NF-κB signaling pathway	1071:1097	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	1	2	theme	pectin	312:317	arg1	complexes					324:332	cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes	271:332	cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes	271:332	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	2	3	theme	activation	607:616	arg1	expression					563:572	the expression	559:572	the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice	559:720	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	5	4	from	effect	1335:1340	arg1	mice					1360:1363	DSS-induced UC mice	1345:1363	DSS-induced UC mice	1345:1363	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	2	5	from	factors	598:604	arg1	flora					684:688	the intestinal flora	669:688	the intestinal flora	669:688	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	5	6	theme	C3G-BP	1275:1280	arg1	complexes					1282:1290	HHP-treated C3G-BP complexes	1263:1290	the HHP-treated C3G-BP complexes group	1259:1296	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	2	7	theme	proteins	644:651	arg1	changes					658:664	changes	658:664	changes in the intestinal flora	658:688	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	7	theme	proteins	644:651	arg1	activation					607:616	activation	607:616	activation of signal pathway-related proteins	607:651	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	7	theme	proteins	644:651	arg1	factors					598:604	inflammation-related factors	577:604	inflammation-related factors	577:604	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	1	8	with	mice	337:340	arg1	colitis					384:390	dextran sodium sulfate (DSS)-induced colitis	347:390	dextran sodium sulfate (DSS)-induced colitis	347:390	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	4	9	theme	improved	1181:1188	arg1	composition					1205:1215	improved gut microbiota composition	1181:1215	improved gut microbiota composition	1181:1215	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	3	10	theme	C3G-BP	759:764	arg1	complexes					766:774	HHP-treated C3G-BP complexes	747:774	HHP-treated C3G-BP complexes	747:774	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	4	11	theme	intestinal	936:945	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	1	12	theme	high	409:412	arg1	pressure					426:433	high hydrostatic pressure	409:433	high hydrostatic pressure (HHP) treatment	409:449	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	1	12	theme	high	409:412	arg1	HHP					436:438	HHP	436:438	HHP	436:438	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	4	13	theme	stress	957:962	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	14	theme	caspase-3	1160:1168	arg1	genes					1170:1174	caspase-3/cleaved caspase-3 genes	1142:1174	caspase-3/cleaved caspase-3 genes	1142:1174	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	6	15	theme	new	1390:1392	arg1	ideas					1394:1398	new ideas	1390:1398	new ideas	1390:1398	The results may provide new ideas for using C3G-BP complexes for treating UC and help develop better processing methods.
35297441	1	16	theme	pressure	426:433	arg1	treatment					441:449	high hydrostatic pressure (HHP) treatment	409:449	high hydrostatic pressure (HHP) treatment	409:449	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	2	17	theme	polymerase	462:471	arg1	RT-PCR					489:494	RT-PCR	489:494	RT-PCR	489:494	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	17	theme	polymerase	462:471	arg1	reaction					479:486	Real-time polymerase chain reaction	452:486	Real-time polymerase chain reaction (RT-PCR)	452:495	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	1	18	theme	cyanidin-3-glucoside	271:290	arg1	complexes					324:332	cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes	271:332	cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes	271:332	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	2	19	theme	factors	598:604	arg1	expression					563:572	the expression	559:572	the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice	559:720	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	5	20	theme	other	1232:1236	arg1	groups					1251:1256	other experimental groups	1232:1256	other experimental groups	1232:1256	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	0	21	theme	ulcerative	189:198	arg1	colitis					200:206	sulfate-induced ulcerative colitis	173:206	sulfate-induced ulcerative colitis	173:206	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	4	22	from	reduction	923:931	arg1	levels					1049:1054	anti-inflammatory factor levels	1024:1054	anti-inflammatory factor levels	1024:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	22	from	reduction	923:931	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	3	23	theme	colon	862:866	arg1	shortening					868:877	colon shortening	862:877	colon shortening	862:877	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	5	24	theme	UC	1357:1358	arg1	mice					1360:1363	DSS-induced UC mice	1345:1363	DSS-induced UC mice	1345:1363	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	4	25	theme	microbiota	1194:1203	arg1	composition					1205:1215	improved gut microbiota composition	1181:1215	improved gut microbiota composition	1181:1215	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	3	26	theme	blood	812:816	arg1	loss					818:821	blood loss	812:821	blood loss	812:821	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	0	27	theme	hydrostatic	74:84	arg1	pressure					86:93	high hydrostatic pressure	69:93	high hydrostatic pressure	69:93	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	5	28	theme	anti-inflammatory	1317:1333	arg1	effect					1335:1340	the best anti-inflammatory effect	1308:1340	the best anti-inflammatory effect on DSS-induced UC mice	1308:1363	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	2	29	used	used	545:548	arg2	RT-PCR					489:494	RT-PCR	489:494	RT-PCR	489:494	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	29	used	used	545:548	arg2	sequencing					529:538	16S rDNA sequencing	520:538	16S rDNA sequencing	520:538	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	29	used	used	545:548	arg2	reaction					479:486	Real-time polymerase chain reaction	452:486	Real-time polymerase chain reaction (RT-PCR)	452:495	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	29	used	used	545:548	arg2	blotting					506:513	western blotting	498:513	western blotting	498:513	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	3	30	from	diarrhea	799:806	arg1	stool					830:834	the stool	826:834	the stool of UC mice	826:845	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	2	31	theme	rDNA	524:527	arg1	sequencing					529:538	16S rDNA sequencing	520:538	16S rDNA sequencing	520:538	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	32	theme	changes	658:664	arg1	expression					563:572	the expression	559:572	the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice	559:720	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	0	33	theme	pectin	138:143	arg1	complexes					145:153	blueberry pectin complexes	128:153	blueberry pectin complexes on dextran sodium	128:171	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	2	34	theme	ulcerative	693:702	arg1	UC					713:714	UC	713:714	UC	713:714	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	34	theme	ulcerative	693:702	arg1	colitis					704:710	ulcerative colitis	693:710	ulcerative colitis (UC) mice	693:720	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	0	35	theme	Anti-inflammatory	0:16	arg1	properties					55:64	Anti-inflammatory and intestinal microbiota modulation properties	0:64	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure	0:93	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	4	36	theme	NF-κB	1075:1079	arg1	pathway					1091:1097	the NF-κB signaling pathway	1071:1097	the NF-κB signaling pathway	1071:1097	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	0	37	theme	intestinal	22:31	arg1	properties					55:64	Anti-inflammatory and intestinal microbiota modulation properties	0:64	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure	0:93	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	5	38	theme	experimental	1238:1249	arg1	groups					1251:1256	other experimental groups	1232:1256	other experimental groups	1232:1256	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	4	39	theme	factor	1042:1047	arg1	levels					1049:1054	anti-inflammatory factor levels	1024:1054	anti-inflammatory factor levels	1024:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	0	40	theme	modulation	44:53	arg1	properties					55:64	Anti-inflammatory and intestinal microbiota modulation properties	0:64	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure	0:93	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	6	41	theme	better	1460:1465	arg1	methods					1478:1484	better processing methods	1460:1484	better processing methods	1460:1484	The results may provide new ideas for using C3G-BP complexes for treating UC and help develop better processing methods.
35297441	4	42	theme	action	907:912	arg1	mechanism					894:902	The potential mechanism	880:902	The potential mechanism of action	880:912	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	5	43	theme	complexes	1282:1290	arg1	group					1292:1296	the HHP-treated C3G-BP complexes group	1259:1296	the HHP-treated C3G-BP complexes group	1259:1296	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	4	44	theme	Bcl-2/Bax	1128:1136	arg1	levels					1118:1123	increased protein levels	1100:1123	increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes	1100:1174	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	44	theme	Bcl-2/Bax	1128:1136	arg1	reduction					923:931	reduction	923:931	reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors	923:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	44	theme	Bcl-2/Bax	1128:1136	arg1	improvement					1009:1019	improvement	1009:1019	improvement in anti-inflammatory factor levels	1009:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	44	theme	Bcl-2/Bax	1128:1136	arg1	composition					1205:1215	improved gut microbiota composition	1181:1215	improved gut microbiota composition	1181:1215	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	44	theme	Bcl-2/Bax	1128:1136	arg1	inhibition					1057:1066	inhibition	1057:1066	inhibition of the NF-κB signaling pathway	1057:1097	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	6	45	theme	processing	1467:1476	arg1	methods					1478:1484	better processing methods	1460:1484	better processing methods	1460:1484	The results may provide new ideas for using C3G-BP complexes for treating UC and help develop better processing methods.
35297441	4	46	theme	pathway	1091:1097	arg1	levels					1118:1123	increased protein levels	1100:1123	increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes	1100:1174	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	46	theme	pathway	1091:1097	arg1	reduction					923:931	reduction	923:931	reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors	923:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	46	theme	pathway	1091:1097	arg1	improvement					1009:1019	improvement	1009:1019	improvement in anti-inflammatory factor levels	1009:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	46	theme	pathway	1091:1097	arg1	composition					1205:1215	improved gut microbiota composition	1181:1215	improved gut microbiota composition	1181:1215	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	46	theme	pathway	1091:1097	arg1	inhibition					1057:1066	inhibition	1057:1066	inhibition of the NF-κB signaling pathway	1057:1097	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	1	47	theme	blueberry	302:310	arg1	pectin					312:317	blueberry pectin	302:317	blueberry pectin (BP)	302:322	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	1	47	theme	blueberry	302:310	arg1	BP					320:321	BP	320:321	BP	320:321	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	4	48	theme	increased	1100:1108	arg1	levels					1118:1123	increased protein levels	1100:1123	increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes	1100:1174	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	2	49	from	expression	563:572	arg1	mice					717:720	ulcerative colitis (UC) mice	693:720	ulcerative colitis (UC) mice	693:720	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	49	from	expression	563:572	arg1	flora					684:688	the intestinal flora	669:688	the intestinal flora	669:688	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	5	50	theme	HHP-treated	1263:1273	arg1	complexes					1282:1290	HHP-treated C3G-BP complexes	1263:1290	the HHP-treated C3G-BP complexes group	1259:1296	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	4	51	theme	potential	884:892	arg1	mechanism					894:902	The potential mechanism	880:902	The potential mechanism of action	880:912	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	3	52	theme	UC	839:840	arg1	mice					842:845	UC mice	839:845	UC mice	839:845	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	4	53	theme	gut	1190:1192	arg1	composition					1205:1215	improved gut microbiota composition	1181:1215	improved gut microbiota composition	1181:1215	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	2	54	theme	pathway-related	628:642	arg1	proteins					644:651	signal pathway-related proteins	621:651	signal pathway-related proteins	621:651	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	4	55	theme	caspase-3/cleaved	1142:1158	arg1	genes					1170:1174	caspase-3/cleaved caspase-3 genes	1142:1174	caspase-3/cleaved caspase-3 genes	1142:1174	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	0	56	theme	dextran	158:164	arg1	sodium					166:171	dextran sodium	158:171	dextran sodium	158:171	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	1	57	theme	anti-inflammatory	242:258	arg1	effects					260:266	the anti-inflammatory effects	238:266	the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis	238:390	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	0	58	from	cyanidin-3-glucoside	103:122	arg1	sodium					166:171	dextran sodium	158:171	dextran sodium	158:171	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	4	59	theme	genes	1170:1174	arg1	levels					1118:1123	increased protein levels	1100:1123	increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes	1100:1174	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	59	theme	genes	1170:1174	arg1	reduction					923:931	reduction	923:931	reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors	923:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	59	theme	genes	1170:1174	arg1	improvement					1009:1019	improvement	1009:1019	improvement in anti-inflammatory factor levels	1009:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	59	theme	genes	1170:1174	arg1	composition					1205:1215	improved gut microbiota composition	1181:1215	improved gut microbiota composition	1181:1215	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	59	theme	genes	1170:1174	arg1	inhibition					1057:1066	inhibition	1057:1066	inhibition of the NF-κB signaling pathway	1057:1097	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	3	60	theme	HHP-treated	747:757	arg1	complexes					766:774	HHP-treated C3G-BP complexes	747:774	HHP-treated C3G-BP complexes	747:774	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	0	61	theme	sulfate-induced	173:187	arg1	colitis					200:206	sulfate-induced ulcerative colitis	173:206	sulfate-induced ulcerative colitis	173:206	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	1	62	theme	hydrostatic	414:424	arg1	pressure					426:433	high hydrostatic pressure	409:433	high hydrostatic pressure (HHP) treatment	409:449	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	1	62	theme	hydrostatic	414:424	arg1	HHP					436:438	HHP	436:438	HHP	436:438	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	4	63	theme	oxidative	947:955	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	2	64	theme	inflammation-related	577:596	arg1	factors					598:604	inflammation-related factors	577:604	inflammation-related factors	577:604	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	0	65	from	complexes	145:153	arg1	sodium					166:171	dextran sodium	158:171	dextran sodium	158:171	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	2	66	from	changes	658:664	arg1	flora					684:688	the intestinal flora	669:688	the intestinal flora	669:688	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	5	67	theme	DSS-induced	1345:1355	arg1	mice					1360:1363	DSS-induced UC mice	1345:1363	DSS-induced UC mice	1345:1363	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	1	68	from	effects	260:266	arg1	mice					337:340	mice	337:340	mice with dextran sodium sulfate (DSS)-induced colitis	337:390	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	4	69	from	levels	1118:1123	arg1	levels					1049:1054	anti-inflammatory factor levels	1024:1054	anti-inflammatory factor levels	1024:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	69	from	levels	1118:1123	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	0	70	theme	pressure	86:93	arg1	properties					55:64	Anti-inflammatory and intestinal microbiota modulation properties	0:64	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure	0:93	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	4	71	theme	factors	1000:1006	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	2	72	from	flora	684:688	arg1	expression					563:572	the expression	559:572	the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice	559:720	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	5	73	theme	best	1312:1315	arg1	effect					1335:1340	the best anti-inflammatory effect	1308:1340	the best anti-inflammatory effect on DSS-induced UC mice	1308:1363	Compared with other experimental groups, the HHP-treated C3G-BP complexes group exhibited the best anti-inflammatory effect on DSS-induced UC mice.
35297441	0	74	theme	cyanidin-3-glucoside	103:122	arg1	mice					208:211	cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice	103:211	cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice	103:211	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	2	75	theme	western	498:504	arg1	blotting					506:513	western blotting	498:513	western blotting	498:513	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	76	theme	intestinal	673:682	arg1	flora					684:688	the intestinal flora	669:688	the intestinal flora	669:688	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	3	77	theme	mice	842:845	arg1	stool					830:834	the stool	826:834	the stool of UC mice	826:845	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	0	78	theme	blueberry	128:136	arg1	complexes					145:153	blueberry pectin complexes	128:153	blueberry pectin complexes on dextran sodium	128:171	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	1	79	theme	-induced	375:382	arg1	colitis					384:390	dextran sodium sulfate (DSS)-induced colitis	347:390	dextran sodium sulfate (DSS)-induced colitis	347:390	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	4	80	theme	mRNA	964:967	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	0	81	theme	complexes	145:153	arg1	mice					208:211	cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice	103:211	cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice	103:211	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	2	82	theme	16S	520:522	arg1	sequencing					529:538	16S rDNA sequencing	520:538	16S rDNA sequencing	520:538	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	83	theme	colitis	704:710	arg1	mice					717:720	ulcerative colitis (UC) mice	693:720	ulcerative colitis (UC) mice	693:720	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	4	84	theme	pro-inflammatory	983:998	arg1	factors					1000:1006	pro-inflammatory factors	983:1006	pro-inflammatory factors	983:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	0	85	dep	cyanidin-3-glucoside	103:122	arg1	colitis					200:206	sulfate-induced ulcerative colitis	173:206	sulfate-induced ulcerative colitis	173:206	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	2	86	theme	chain	473:477	arg1	RT-PCR					489:494	RT-PCR	489:494	RT-PCR	489:494	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	86	theme	chain	473:477	arg1	reaction					479:486	Real-time polymerase chain reaction	452:486	Real-time polymerase chain reaction (RT-PCR)	452:495	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	87	from	activation	607:616	arg1	flora					684:688	the intestinal flora	669:688	the intestinal flora	669:688	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	0	88	theme	microbiota	33:42	arg1	properties					55:64	Anti-inflammatory and intestinal microbiota modulation properties	0:64	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure	0:93	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	1	89	theme	complexes	324:332	arg1	effects					260:266	the anti-inflammatory effects	238:266	the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis	238:390	This study investigated the anti-inflammatory effects of cyanidin-3-glucoside (C3G) and blueberry pectin (BP) complexes on mice with dextran sodium sulfate (DSS)-induced colitis before and after high hydrostatic pressure (HHP) treatment.
35297441	2	90	theme	Real-time	452:460	arg1	RT-PCR					489:494	RT-PCR	489:494	RT-PCR	489:494	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	2	90	theme	Real-time	452:460	arg1	reaction					479:486	Real-time polymerase chain reaction	452:486	Real-time polymerase chain reaction (RT-PCR)	452:495	Real-time polymerase chain reaction (RT-PCR), western blotting, and 16S rDNA sequencing were used to study the expression of inflammation-related factors, activation of signal pathway-related proteins, and changes in the intestinal flora in ulcerative colitis (UC) mice.
35297441	4	91	from	inhibition	1057:1066	arg1	levels					1049:1054	anti-inflammatory factor levels	1024:1054	anti-inflammatory factor levels	1024:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	91	from	inhibition	1057:1066	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	92	theme	anti-inflammatory	1024:1040	arg1	levels					1049:1054	anti-inflammatory factor levels	1024:1054	anti-inflammatory factor levels	1024:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	6	93	theme	C3G-BP	1410:1415	arg1	complexes					1417:1425	C3G-BP complexes	1410:1425	C3G-BP complexes	1410:1425	The results may provide new ideas for using C3G-BP complexes for treating UC and help develop better processing methods.
35297441	0	94	theme	high	69:72	arg1	pressure					86:93	high hydrostatic pressure	69:93	high hydrostatic pressure	69:93	Anti-inflammatory and intestinal microbiota modulation properties of high hydrostatic pressure treated cyanidin-3-glucoside and blueberry pectin complexes on dextran sodium sulfate-induced ulcerative colitis mice.
35297441	3	95	from	loss	818:821	arg1	stool					830:834	the stool	826:834	the stool of UC mice	826:845	The results showed that HHP-treated C3G-BP complexes significantly relieved diarrhea and blood loss in the stool of UC mice and alleviated colon shortening.
35297441	4	96	theme	protein	1110:1116	arg1	levels					1118:1123	increased protein levels	1100:1123	increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes	1100:1174	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	97	from	improvement	1009:1019	arg1	levels					1049:1054	anti-inflammatory factor levels	1024:1054	anti-inflammatory factor levels	1024:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	97	from	improvement	1009:1019	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	98	from	composition	1205:1215	arg1	levels					1049:1054	anti-inflammatory factor levels	1024:1054	anti-inflammatory factor levels	1024:1054	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
35297441	4	98	from	composition	1205:1215	arg1	expression					969:978	intestinal oxidative stress mRNA expression	936:978	intestinal oxidative stress mRNA expression of pro-inflammatory factors	936:1006	The potential mechanism of action involved reduction in intestinal oxidative stress mRNA expression of pro-inflammatory factors, improvement in anti-inflammatory factor levels, inhibition of the NF-κB signaling pathway, increased protein levels of Bcl-2/Bax and caspase-3/cleaved caspase-3 genes, and improved gut microbiota composition.
36112615	4	0	theme	same	915:918	arg1	diet					933:936	the same high protein diet	911:936	the same high protein diet	911:936	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	7	1	theme	fish	1310:1313	arg1	scales					1315:1320	fish scales	1310:1320	fish scales	1310:1320	These bacteria sometimes digest collagen, the major component of fish scales, supporting an adaptive shift.
36112615	1	2	theme	niche	276:280	arg1	specialization					282:295	host niche specialization	271:295	host niche specialization	271:295	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	4	3	theme	protein	925:931	arg1	diet					933:936	the same high protein diet	911:936	the same high protein diet	911:936	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	2	4	theme	laboratory	303:312	arg1	garden					321:326	a laboratory common garden	301:326	a laboratory common garden	301:326	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	9	5	from	diversification	1641:1655	arg1	traits					1678:1683	all other trophic traits	1660:1683	all other trophic traits	1660:1683	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	5	from	diversification	1641:1655	arg1	expression					1776:1785	gene expression	1771:1785	gene expression	1771:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	5	from	diversification	1641:1655	arg1	aggression					1755:1764	aggression	1755:1764	aggression	1755:1764	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	5	from	diversification	1641:1655	arg1	morphology					1724:1733	craniofacial morphology	1711:1733	craniofacial morphology	1711:1733	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	5	from	diversification	1641:1655	arg1	behavior					1745:1752	foraging behavior	1736:1752	foraging behavior	1736:1752	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	6	theme	Overall	1499:1505	arg1	conservation					1520:1531	Overall phylogenetic conservation	1499:1531	Overall phylogenetic conservation of microbiome composition	1499:1557	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	1	7	theme	excellent	183:191	arg1	opportunity					193:203	an excellent opportunity	180:203	an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization	180:295	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	6	8	theme	scale-eater	1220:1230	arg1	populations					1232:1242	replicated lab-reared scale-eater populations	1198:1242	replicated lab-reared scale-eater populations	1198:1242	However, we did find significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations.
36112615	2	9	theme	stable	565:570	arg1	diets					583:587	stable generalist diets	565:587	stable generalist diets persisting over 11 Mya	565:610	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	6	10	theme	replicated	1198:1207	arg1	populations					1232:1242	replicated lab-reared scale-eater populations	1198:1242	replicated lab-reared scale-eater populations	1198:1242	However, we did find significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations.
36112615	3	11	theme	diversification	769:783	arg1	rates					732:736	their rapid rates	720:736	their rapid rates of craniofacial and behavioral diversification	720:783	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	0	12	theme	adaptive	116:123	arg1	enrichment					125:134	potential adaptive enrichment	106:134	potential adaptive enrichment for scale-eating	106:151	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	5	13	theme	phylosymbiosis	1106:1119	arg1	support					1095:1101	support	1095:1101	support of phylosymbiosis	1095:1119	In contrast to our predictions, gut microbiota largely reflected phylogenetic distance among species, rather than generalist or specialist life history, in support of phylosymbiosis.
36112615	9	14	theme	phylogenetic	1507:1518	arg1	conservation					1520:1531	Overall phylogenetic conservation	1499:1531	Overall phylogenetic conservation of microbiome composition	1499:1557	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	15	theme	radiation	1598:1606	arg1	theory					1608:1613	adaptive radiation theory	1589:1613	adaptive radiation theory	1589:1613	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	16	theme	shifts	1839:1844	arg1	role					1819:1822	the functional role	1804:1822	the functional role of these minor shifts in microbiota	1804:1858	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	16	theme	shifts	1839:1844	arg1	important					1868:1876	important	1868:1876	important	1868:1876	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	17	theme	diversification	1641:1655	arg1	observations					1619:1630	observations	1619:1630	observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression	1619:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	17	theme	diversification	1641:1655	arg1	predictions					1574:1584	predictions	1574:1584	predictions of adaptive radiation theory	1574:1613	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	18	from	traits	1678:1683	arg1	observations					1619:1630	observations	1619:1630	observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression	1619:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	18	from	traits	1678:1683	arg1	hosts					1694:1698	these hosts	1688:1698	these hosts	1688:1698	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	18	from	traits	1678:1683	arg1	predictions					1574:1584	predictions	1574:1584	predictions of adaptive radiation theory	1574:1613	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	0	19	from	divergence	16:25	arg1	radiation					64:72	an adaptive radiation	52:72	an adaptive radiation of Cyprinodon	52:86	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	7	20	theme	major	1291:1295	arg1	collagen					1277:1284	collagen	1277:1284	collagen	1277:1284	These bacteria sometimes digest collagen, the major component of fish scales, supporting an adaptive shift.
36112615	7	20	theme	major	1291:1295	arg1	component					1297:1305	the major component	1287:1305	the major component of fish scales	1287:1320	These bacteria sometimes digest collagen, the major component of fish scales, supporting an adaptive shift.
36112615	4	21	theme	rRNA	803:806	arg1	amplicons					808:816	16S rRNA amplicons	799:816	16S rRNA amplicons of gut microbiomes	799:835	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	0	22	theme	Cyprinodon	77:86	arg1	radiation					64:72	an adaptive radiation	52:72	an adaptive radiation of Cyprinodon	52:86	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	9	23	theme	trophic	1670:1676	arg1	traits					1678:1683	all other trophic traits	1660:1683	all other trophic traits	1660:1683	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	23	theme	trophic	1670:1676	arg1	expression					1776:1785	gene expression	1771:1785	gene expression	1771:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	23	theme	trophic	1670:1676	arg1	aggression					1755:1764	aggression	1755:1764	aggression	1755:1764	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	23	theme	trophic	1670:1676	arg1	morphology					1724:1733	craniofacial morphology	1711:1733	craniofacial morphology	1711:1733	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	23	theme	trophic	1670:1676	arg1	behavior					1745:1752	foraging behavior	1736:1752	foraging behavior	1736:1752	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	2	24	theme	trophic	529:535	arg1	evolution					537:545	rapid trophic evolution	523:545	rapid trophic evolution within 10 kya	523:559	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	8	25	theme	molluscivore	1436:1447	arg1	populations					1449:1459	lab-reared molluscivore populations	1425:1459	lab-reared molluscivore populations	1425:1459	We also found some enrichment of Rhodobacteraceae and Planctomycetia in lab-reared molluscivore populations, but these bacteria target cellulose.
36112615	9	26	theme	microbiome	1912:1921	arg1	role					1900:1903	the role	1896:1903	the role of the microbiome in trophic diversification	1896:1948	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	2	27	theme	pupfishes	400:408	arg1	microbiota					349:358	the gut microbiota	341:358	the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations	341:506	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	0	28	theme	Minimal	0:6	arg1	divergence					16:25	Minimal overall divergence	0:25	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon	0:86	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	9	29	theme	trophic	1926:1932	arg1	diversification					1934:1948	trophic diversification	1926:1948	trophic diversification	1926:1948	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	2	30	theme	generalist	485:494	arg1	populations					496:506	closely related and distant outgroup generalist populations	448:506	closely related and distant outgroup generalist populations	448:506	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	31	theme	distant	468:474	arg1	populations					496:506	closely related and distant outgroup generalist populations	448:506	closely related and distant outgroup generalist populations	448:506	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	3	32	theme	divergent	649:657	arg1	composition					670:680	an adaptive and highly divergent microbiome composition	626:680	an adaptive and highly divergent microbiome composition	626:680	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	6	33	from	enrichment	1155:1164	arg1	populations					1232:1242	replicated lab-reared scale-eater populations	1198:1242	replicated lab-reared scale-eater populations	1198:1242	However, we did find significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations.
36112615	0	34	theme	microbiome	38:47	arg1	divergence					16:25	Minimal overall divergence	0:25	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon	0:86	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	9	35	theme	foraging	1736:1743	arg1	behavior					1745:1752	foraging behavior	1736:1752	foraging behavior	1736:1752	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	36	from	predictions	1574:1584	arg1	traits					1678:1683	all other trophic traits	1660:1683	all other trophic traits	1660:1683	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	36	from	predictions	1574:1584	arg1	expression					1776:1785	gene expression	1771:1785	gene expression	1771:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	36	from	predictions	1574:1584	arg1	aggression					1755:1764	aggression	1755:1764	aggression	1755:1764	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	36	from	predictions	1574:1584	arg1	morphology					1724:1733	craniofacial morphology	1711:1733	craniofacial morphology	1711:1733	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	36	from	predictions	1574:1584	arg1	behavior					1745:1752	foraging behavior	1736:1752	foraging behavior	1736:1752	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	6	37	theme	Burkholderiaceae	1169:1184	arg1	bacteria					1186:1193	Burkholderiaceae bacteria	1169:1193	Burkholderiaceae bacteria	1169:1193	However, we did find significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations.
36112615	4	38	theme	microbiomes	825:835	arg1	amplicons					808:816	16S rRNA amplicons	799:816	16S rRNA amplicons of gut microbiomes	799:835	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	2	39	theme	gut	345:347	arg1	microbiota					349:358	the gut microbiota	341:358	the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations	341:506	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	7	40	theme	adaptive	1337:1344	arg1	shift					1346:1350	an adaptive shift	1334:1350	an adaptive shift	1334:1350	These bacteria sometimes digest collagen, the major component of fish scales, supporting an adaptive shift.
36112615	4	41	theme	lab-reared	842:851	arg1	pupfishes					859:867	lab-reared adult pupfishes	842:867	lab-reared adult pupfishes raised under identical conditions	842:901	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	2	42	theme	trophic	381:387	arg1	pupfishes					400:408	two novel derived trophic specialist pupfishes	363:408	two novel derived trophic specialist pupfishes	363:408	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	42	theme	trophic	381:387	arg1	scale-eater					413:423	a scale-eater	411:423	a scale-eater	411:423	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	42	theme	trophic	381:387	arg1	molluscivore					431:442	a molluscivore	429:442	a molluscivore	429:442	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	9	43	theme	composition	1547:1557	arg1	conservation					1520:1531	Overall phylogenetic conservation	1499:1531	Overall phylogenetic conservation of microbiome composition	1499:1557	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	1	44	theme	microbiota	256:265	arg1	dynamics					240:247	the eco-evolutionary dynamics	219:247	the eco-evolutionary dynamics of gut microbiota and host niche specialization	219:295	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	2	45	link	derived	373:379	arg1	pupfishes					400:408	two novel derived trophic specialist pupfishes	363:408	two novel derived trophic specialist pupfishes	363:408	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	45	link	derived	373:379	arg1	scale-eater					413:423	a scale-eater	411:423	a scale-eater	411:423	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	45	link	derived	373:379	arg1	molluscivore					431:442	a molluscivore	429:442	a molluscivore	429:442	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	46	theme	novel	367:371	arg1	pupfishes					400:408	two novel derived trophic specialist pupfishes	363:408	two novel derived trophic specialist pupfishes	363:408	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	46	theme	novel	367:371	arg1	scale-eater					413:423	a scale-eater	411:423	a scale-eater	411:423	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	46	theme	novel	367:371	arg1	molluscivore					431:442	a molluscivore	429:442	a molluscivore	429:442	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	1	47	theme	host	271:274	arg1	specialization					282:295	host niche specialization	271:295	host niche specialization	271:295	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	9	48	theme	gene	1771:1774	arg1	expression					1776:1785	gene expression	1771:1785	gene expression	1771:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	7	49	theme	scales	1315:1320	arg1	collagen					1277:1284	collagen	1277:1284	collagen	1277:1284	These bacteria sometimes digest collagen, the major component of fish scales, supporting an adaptive shift.
36112615	7	49	theme	scales	1315:1320	arg1	component					1297:1305	the major component	1287:1305	the major component of fish scales	1287:1320	These bacteria sometimes digest collagen, the major component of fish scales, supporting an adaptive shift.
36112615	2	50	theme	common	314:319	arg1	garden					321:326	a laboratory common garden	301:326	a laboratory common garden	301:326	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	5	51	from	distance	1017:1024	arg1	support					1095:1101	support	1095:1101	support of phylosymbiosis	1095:1119	In contrast to our predictions, gut microbiota largely reflected phylogenetic distance among species, rather than generalist or specialist life history, in support of phylosymbiosis.
36112615	1	52	theme	specialization	282:295	arg1	dynamics					240:247	the eco-evolutionary dynamics	219:247	the eco-evolutionary dynamics of gut microbiota and host niche specialization	219:295	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	4	53	theme	high	920:923	arg1	diet					933:936	the same high protein diet	911:936	the same high protein diet	911:936	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	3	54	theme	behavioral	758:767	arg1	diversification					769:783	craniofacial and behavioral diversification	741:783	craniofacial and behavioral diversification	741:783	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	9	55	theme	microbiome	1536:1545	arg1	composition					1547:1557	microbiome composition	1536:1557	microbiome composition	1536:1557	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	4	56	theme	identical	882:890	arg1	conditions					892:901	identical conditions	882:901	identical conditions	882:901	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	9	57	from	observations	1619:1630	arg1	traits					1678:1683	all other trophic traits	1660:1683	all other trophic traits	1660:1683	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	57	from	observations	1619:1630	arg1	expression					1776:1785	gene expression	1771:1785	gene expression	1771:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	57	from	observations	1619:1630	arg1	aggression					1755:1764	aggression	1755:1764	aggression	1755:1764	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	57	from	observations	1619:1630	arg1	morphology					1724:1733	craniofacial morphology	1711:1733	craniofacial morphology	1711:1733	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	57	from	observations	1619:1630	arg1	behavior					1745:1752	foraging behavior	1736:1752	foraging behavior	1736:1752	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	3	58	theme	craniofacial	741:752	arg1	diversification					769:783	craniofacial and behavioral diversification	741:783	craniofacial and behavioral diversification	741:783	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	9	59	theme	functional	1808:1817	arg1	role					1819:1822	the functional role	1804:1822	the functional role of these minor shifts in microbiota	1804:1858	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	59	theme	functional	1808:1817	arg1	important					1868:1876	important	1868:1876	important	1868:1876	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	6	60	theme	lab-reared	1209:1218	arg1	populations					1232:1242	replicated lab-reared scale-eater populations	1198:1242	replicated lab-reared scale-eater populations	1198:1242	However, we did find significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations.
36112615	5	61	dep	generalist	1053:1062	arg1	history					1083:1089	life history	1078:1089	life history	1078:1089	In contrast to our predictions, gut microbiota largely reflected phylogenetic distance among species, rather than generalist or specialist life history, in support of phylosymbiosis.
36112615	1	62	theme	eco-evolutionary	223:238	arg1	dynamics					240:247	the eco-evolutionary dynamics	219:247	the eco-evolutionary dynamics of gut microbiota and host niche specialization	219:295	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	9	63	theme	minor	1833:1837	arg1	shifts					1839:1844	these minor shifts	1827:1844	these minor shifts	1827:1844	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	64	theme	theory	1608:1613	arg1	observations					1619:1630	observations	1619:1630	observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression	1619:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	64	theme	theory	1608:1613	arg1	predictions					1574:1584	predictions	1574:1584	predictions of adaptive radiation theory	1574:1613	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	3	65	theme	adaptive	629:636	arg1	composition					670:680	an adaptive and highly divergent microbiome composition	626:680	an adaptive and highly divergent microbiome composition	626:680	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	2	66	theme	generalist	572:581	arg1	diets					583:587	stable generalist diets	565:587	stable generalist diets persisting over 11 Mya	565:610	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	9	67	theme	other	1664:1668	arg1	traits					1678:1683	all other trophic traits	1660:1683	all other trophic traits	1660:1683	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	67	theme	other	1664:1668	arg1	expression					1776:1785	gene expression	1771:1785	gene expression	1771:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	67	theme	other	1664:1668	arg1	aggression					1755:1764	aggression	1755:1764	aggression	1755:1764	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	67	theme	other	1664:1668	arg1	morphology					1724:1733	craniofacial morphology	1711:1733	craniofacial morphology	1711:1733	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	67	theme	other	1664:1668	arg1	behavior					1745:1752	foraging behavior	1736:1752	foraging behavior	1736:1752	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	0	68	theme	adaptive	55:62	arg1	radiation					64:72	an adaptive radiation	52:72	an adaptive radiation of Cyprinodon	52:86	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	9	69	theme	rapid	1635:1639	arg1	diversification					1641:1655	rapid diversification	1635:1655	rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression	1635:1785	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	8	70	theme	Rhodobacteraceae	1386:1401	arg1	enrichment					1372:1381	some enrichment	1367:1381	some enrichment of Rhodobacteraceae and Planctomycetia in lab-reared molluscivore populations	1367:1459	We also found some enrichment of Rhodobacteraceae and Planctomycetia in lab-reared molluscivore populations, but these bacteria target cellulose.
36112615	5	71	theme	phylogenetic	1004:1015	arg1	distance					1017:1024	phylogenetic distance	1004:1024	phylogenetic distance among species, rather than generalist or specialist life history, in support of phylosymbiosis	1004:1119	In contrast to our predictions, gut microbiota largely reflected phylogenetic distance among species, rather than generalist or specialist life history, in support of phylosymbiosis.
36112615	9	72	from	role	1819:1822	arg1	microbiota					1849:1858	microbiota	1849:1858	microbiota	1849:1858	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	8	73	theme	Planctomycetia	1407:1420	arg1	enrichment					1372:1381	some enrichment	1367:1381	some enrichment of Rhodobacteraceae and Planctomycetia in lab-reared molluscivore populations	1367:1459	We also found some enrichment of Rhodobacteraceae and Planctomycetia in lab-reared molluscivore populations, but these bacteria target cellulose.
36112615	4	74	theme	16S	799:801	arg1	rRNA					803:806	16S rRNA	799:806	16S rRNA amplicons of gut microbiomes	799:835	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	0	75	theme	potential	106:114	arg1	enrichment					125:134	potential adaptive enrichment	106:134	potential adaptive enrichment for scale-eating	106:151	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	2	76	theme	rapid	523:527	arg1	evolution					537:545	rapid trophic evolution	523:545	rapid trophic evolution within 10 kya	523:559	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	8	77	theme	lab-reared	1425:1434	arg1	populations					1449:1459	lab-reared molluscivore populations	1425:1459	lab-reared molluscivore populations	1425:1459	We also found some enrichment of Rhodobacteraceae and Planctomycetia in lab-reared molluscivore populations, but these bacteria target cellulose.
36112615	0	78	theme	overall	8:14	arg1	divergence					16:25	Minimal overall divergence	0:25	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon	0:86	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	5	79	theme	gut	971:973	arg1	microbiota					975:984	gut microbiota	971:984	gut microbiota	971:984	In contrast to our predictions, gut microbiota largely reflected phylogenetic distance among species, rather than generalist or specialist life history, in support of phylosymbiosis.
36112615	2	80	theme	specialist	389:398	arg1	pupfishes					400:408	two novel derived trophic specialist pupfishes	363:408	two novel derived trophic specialist pupfishes	363:408	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	80	theme	specialist	389:398	arg1	scale-eater					413:423	a scale-eater	411:423	a scale-eater	411:423	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	80	theme	specialist	389:398	arg1	molluscivore					431:442	a molluscivore	429:442	a molluscivore	429:442	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	8	81	from	enrichment	1372:1381	arg1	populations					1449:1459	lab-reared molluscivore populations	1425:1459	lab-reared molluscivore populations	1425:1459	We also found some enrichment of Rhodobacteraceae and Planctomycetia in lab-reared molluscivore populations, but these bacteria target cellulose.
36112615	3	82	theme	microbiome	659:668	arg1	composition					670:680	an adaptive and highly divergent microbiome composition	626:680	an adaptive and highly divergent microbiome composition	626:680	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	0	83	theme	gut	34:36	arg1	microbiome					38:47	the gut microbiome	30:47	the gut microbiome	30:47	Minimal overall divergence of the gut microbiome in an adaptive radiation of Cyprinodon pupfishes despite potential adaptive enrichment for scale-eating.
36112615	9	84	theme	adaptive	1589:1596	arg1	theory					1608:1613	adaptive radiation theory	1589:1613	adaptive radiation theory	1589:1613	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	9	85	theme	craniofacial	1711:1722	arg1	morphology					1724:1733	craniofacial morphology	1711:1733	craniofacial morphology	1711:1733	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	2	86	theme	outgroup	476:483	arg1	populations					496:506	closely related and distant outgroup generalist populations	448:506	closely related and distant outgroup generalist populations	448:506	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	4	87	theme	adult	853:857	arg1	pupfishes					859:867	lab-reared adult pupfishes	842:867	lab-reared adult pupfishes raised under identical conditions	842:901	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	9	88	from	role	1900:1903	arg1	diversification					1934:1948	trophic diversification	1926:1948	trophic diversification	1926:1948	Overall phylogenetic conservation of microbiome composition contrasts with predictions of adaptive radiation theory and observations of rapid diversification in all other trophic traits in these hosts, including craniofacial morphology, foraging behavior, aggression, and gene expression, suggesting that the functional role of these minor shifts in microbiota will be important for understanding the role of the microbiome in trophic diversification.
36112615	5	89	theme	life	1078:1081	arg1	history					1083:1089	life history	1078:1089	life history	1078:1089	In contrast to our predictions, gut microbiota largely reflected phylogenetic distance among species, rather than generalist or specialist life history, in support of phylosymbiosis.
36112615	6	90	theme	bacteria	1186:1193	arg1	enrichment					1155:1164	significant enrichment	1143:1164	significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations	1143:1242	However, we did find significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations.
36112615	3	91	theme	trophic	689:695	arg1	specialists					697:707	the trophic specialists	685:707	the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification	685:783	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	1	92	theme	Adaptive	154:161	arg1	radiations					163:172	Adaptive radiations	154:172	Adaptive radiations	154:172	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	4	93	theme	gut	821:823	arg1	microbiomes					825:835	gut microbiomes	821:835	gut microbiomes	821:835	We sequenced 16S rRNA amplicons of gut microbiomes from lab-reared adult pupfishes raised under identical conditions and fed the same high protein diet.
36112615	2	94	theme	derived	373:379	arg1	pupfishes					400:408	two novel derived trophic specialist pupfishes	363:408	two novel derived trophic specialist pupfishes	363:408	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	94	theme	derived	373:379	arg1	scale-eater					413:423	a scale-eater	411:423	a scale-eater	411:423	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	2	94	theme	derived	373:379	arg1	molluscivore					431:442	a molluscivore	429:442	a molluscivore	429:442	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36112615	1	95	theme	gut	252:254	arg1	microbiota					256:265	gut microbiota	252:265	gut microbiota	252:265	Adaptive radiations offer an excellent opportunity to understand the eco-evolutionary dynamics of gut microbiota and host niche specialization.
36112615	6	96	theme	significant	1143:1153	arg1	enrichment					1155:1164	significant enrichment	1143:1164	significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations	1143:1242	However, we did find significant enrichment of Burkholderiaceae bacteria in replicated lab-reared scale-eater populations.
36112615	3	97	theme	rapid	726:730	arg1	rates					732:736	their rapid rates	720:736	their rapid rates of craniofacial and behavioral diversification	720:783	We predicted an adaptive and highly divergent microbiome composition in the trophic specialists reflecting their rapid rates of craniofacial and behavioral diversification.
36112615	2	98	theme	related	456:462	arg1	populations					496:506	closely related and distant outgroup generalist populations	448:506	closely related and distant outgroup generalist populations	448:506	In a laboratory common garden, we compared the gut microbiota of two novel derived trophic specialist pupfishes, a scale-eater and a molluscivore, to closely related and distant outgroup generalist populations, spanning both rapid trophic evolution within 10 kya and stable generalist diets persisting over 11 Mya.
36176029	5	0	theme	host-microbe	516:527	arg1	interactions					529:540	host-microbe interactions	516:540	host-microbe interactions in colitis	516:551	However, the roles of EVs-miRNAs and MVs in host-microbe interactions in colitis remain unclear.
36176029	1	1	theme	global	148:153	arg1	disease					129:135	Inflammatory bowel disease	110:135	Inflammatory bowel disease (IBD)	110:141	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	1	1	theme	global	148:153	arg1	disease					155:161	a global disease	146:161	a global disease with no cure	146:174	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	4	2	link	host-derived	365:376	arg1	EVs					378:380	host-derived EVs	365:380	host-derived EVs	365:380	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	10	3	theme	microbiota	1217:1226	arg1	composition					1187:1197	the composition	1183:1197	the composition of the intestinal microbiota	1183:1226	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	3	4	theme	vital	295:299	arg1	participants					301:312	vital participants	295:312	vital participants in cell-cell and cell-organism communication	295:357	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	3	4	theme	vital	295:299	arg1	vesicles					276:283	Extracellular vesicles	262:283	Extracellular vesicles (EVs)	262:289	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	1	5	with	disease	155:161	arg1	cure					171:174	no cure	168:174	no cure	168:174	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	4	6	theme	bacteria-derived	386:401	arg1	OMVs					422:425	OMVs	422:425	OMVs	422:425	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	4	6	theme	bacteria-derived	386:401	arg1	vesicles					412:419	bacteria-derived membrane vesicles	386:419	bacteria-derived membrane vesicles (OMVs)	386:426	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	8	7	dep	inflammation	909:920	arg1	both					922:925	both	922:925	both	922:925	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	8	8	theme	M1	829:830	arg1	polarization					843:854	M1 macrophage polarization	829:854	M1 macrophage polarization	829:854	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	3	9	theme	cell-organism	331:343	arg1	communication					345:357	cell-cell and cell-organism communication	317:357	cell-cell and cell-organism communication	317:357	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	10	10	from	colitis	1294:1300	arg1	BMVs					1284:1287	BMVs	1284:1287	BMVs from colitis	1284:1300	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	6	11	dep	sulfate	646:652	arg1	DSS					662:664	DSS	662:664	DSS	662:664	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	6	11	dep	sulfate	646:652	arg1	sodium					654:659	sodium	654:659	dextran sulfate sodium (DSS)	638:665	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	6	12	theme	animal	595:600	arg1	model					602:606	the animal model	591:606	the animal model of colitis	591:617	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	8	13	theme	promoted	860:867	arg1	polarization					872:883	promoted M2 polarization	860:883	promoted M2 polarization	860:883	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	8	14	theme	M2	869:870	arg1	polarization					872:883	promoted M2 polarization	860:883	promoted M2 polarization	860:883	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	3	15	theme	cell-cell	317:325	arg1	communication					345:357	cell-cell and cell-organism communication	317:357	cell-cell and cell-organism communication	317:357	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	4	16	theme	membrane	403:410	arg1	OMVs					422:425	OMVs	422:425	OMVs	422:425	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	4	16	theme	membrane	403:410	arg1	vesicles					412:419	bacteria-derived membrane vesicles	386:419	bacteria-derived membrane vesicles (OMVs)	386:426	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	2	17	theme	IBD	257:259	arg1	Disruption					177:186	Disruption	177:186	Disruption of the microbial ecosystem	177:213	Disruption of the microbial ecosystem is considered to be an important cause of IBD.
36176029	2	17	theme	IBD	257:259	arg1	cause					248:252	an important cause	235:252	an important cause of IBD	235:259	Disruption of the microbial ecosystem is considered to be an important cause of IBD.
36176029	11	18	theme	new	1468:1470	arg1	ideas					1472:1476	new ideas	1468:1476	new ideas for colitis treatment	1468:1498	Our results preliminarily identify a vesicle-based host-microbe interaction cycle in colitis and provide new ideas for colitis treatment.
36176029	11	19	theme	interaction	1427:1437	arg1	cycle					1439:1443	a vesicle-based host-microbe interaction cycle	1398:1443	a vesicle-based host-microbe interaction cycle	1398:1443	Our results preliminarily identify a vesicle-based host-microbe interaction cycle in colitis and provide new ideas for colitis treatment.
36176029	10	20	theme	intestinal	1241:1250	arg1	barrier					1252:1258	the intestinal barrier	1237:1258	the intestinal barrier	1237:1258	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	6	21	theme	present	576:582	arg1	study					584:588	the present study	572:588	the present study	572:588	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	0	22	theme	vesicle	14:20	arg1	miRNAs					22:27	Extracellular vesicle miRNAs	0:27	Extracellular vesicle miRNAs	0:27	Extracellular vesicle miRNAs promote the intestinal microenvironment by interacting with microbes in colitis.
36176029	10	23	contain	have	1313:1316	arg1	BMVs					1284:1287	BMVs	1284:1287	BMVs from colitis	1284:1300	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	10	23	contain	have	1313:1316	arg2	effects					1326:1332	similar effects	1318:1332	similar effects	1318:1332	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	5	24	theme	EVs-miRNAs	494:503	arg1	roles					485:489	the roles	481:489	the roles of EVs-miRNAs and MVs in host-microbe interactions in colitis	481:551	However, the roles of EVs-miRNAs and MVs in host-microbe interactions in colitis remain unclear.
36176029	0	25	theme	Extracellular	0:12	arg1	miRNAs					22:27	Extracellular vesicle miRNAs	0:27	Extracellular vesicle miRNAs	0:27	Extracellular vesicle miRNAs promote the intestinal microenvironment by interacting with microbes in colitis.
36176029	10	26	from	feces	1151:1155	arg1	MVs					1135:1137	MVs	1135:1137	MVs from normal feces	1135:1155	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	8	27	theme	chronic	953:959	arg1	colitis					961:967	chronic colitis	953:967	chronic colitis	953:967	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	9	28	theme	barrier	1091:1097	arg1	integrity					1099:1107	intestinal barrier integrity	1080:1107	intestinal barrier integrity	1080:1107	miR-200b-3p could interact with bacteria and regulate the composition of the microbiota, which contributed to intestinal barrier integrity and homeostasis.
36176029	10	29	theme	miR-200b-3p	1340:1350	arg1	treatment					1352:1360	miR-200b-3p treatment	1340:1360	miR-200b-3p treatment	1340:1360	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	7	30	theme	colitis	778:784	arg1	EVs					786:788	colitis EVs	778:788	colitis EVs	778:788	Several miRNAs were significantly altered in colitis EVs.
36176029	4	31	theme	host-derived	365:376	arg1	EVs					378:380	host-derived EVs	365:380	host-derived EVs	365:380	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	1	32	theme	Inflammatory	110:121	arg1	IBD					138:140	IBD	138:140	IBD	138:140	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	1	32	theme	Inflammatory	110:121	arg1	disease					129:135	Inflammatory bowel disease	110:135	Inflammatory bowel disease (IBD)	110:141	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	1	32	theme	Inflammatory	110:121	arg1	disease					155:161	a global disease	146:161	a global disease with no cure	146:174	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	8	33	theme	colitis	961:967	arg1	acute					930:934	acute	930:934	acute	930:934	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	6	34	theme	miRNAs	697:702	arg1	changes					686:692	the changes	682:692	the changes of miRNAs in colonic EVs from colitis	682:730	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	3	35	theme	Extracellular	262:274	arg1	participants					301:312	vital participants	295:312	vital participants in cell-cell and cell-organism communication	295:357	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	3	35	theme	Extracellular	262:274	arg1	EVs					286:288	EVs	286:288	EVs	286:288	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	3	35	theme	Extracellular	262:274	arg1	vesicles					276:283	Extracellular vesicles	262:283	Extracellular vesicles (EVs)	262:289	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	1	36	theme	bowel	123:127	arg1	IBD					138:140	IBD	138:140	IBD	138:140	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	1	36	theme	bowel	123:127	arg1	disease					129:135	Inflammatory bowel disease	110:135	Inflammatory bowel disease (IBD)	110:141	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	1	36	theme	bowel	123:127	arg1	disease					155:161	a global disease	146:161	a global disease with no cure	146:174	Inflammatory bowel disease (IBD) is a global disease with no cure.
36176029	11	37	theme	colitis	1482:1488	arg1	treatment					1490:1498	colitis treatment	1482:1498	colitis treatment	1482:1498	Our results preliminarily identify a vesicle-based host-microbe interaction cycle in colitis and provide new ideas for colitis treatment.
36176029	0	38	theme	intestinal	41:50	arg1	microenvironment					52:67	the intestinal microenvironment	37:67	the intestinal microenvironment	37:67	Extracellular vesicle miRNAs promote the intestinal microenvironment by interacting with microbes in colitis.
36176029	0	39	from	microbes	89:96	arg1	colitis					101:107	colitis	101:107	colitis	101:107	Extracellular vesicle miRNAs promote the intestinal microenvironment by interacting with microbes in colitis.
36176029	9	40	theme	microbiota	1047:1056	arg1	composition					1028:1038	the composition	1024:1038	the composition of the microbiota, which contributed to intestinal barrier integrity and homeostasis	1024:1123	miR-200b-3p could interact with bacteria and regulate the composition of the microbiota, which contributed to intestinal barrier integrity and homeostasis.
36176029	5	41	from	interactions	529:540	arg1	colitis					545:551	colitis	545:551	colitis	545:551	However, the roles of EVs-miRNAs and MVs in host-microbe interactions in colitis remain unclear.
36176029	9	42	theme	intestinal	1080:1089	arg1	integrity					1099:1107	intestinal barrier integrity	1080:1107	intestinal barrier integrity	1080:1107	miR-200b-3p could interact with bacteria and regulate the composition of the microbiota, which contributed to intestinal barrier integrity and homeostasis.
36176029	10	43	theme	intestinal	1206:1215	arg1	microbiota					1217:1226	the intestinal microbiota	1202:1226	the intestinal microbiota	1202:1226	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	8	44	theme	inflammation	909:920	arg1	levels					899:904	the levels	895:904	the levels of inflammation both in acute and remission of chronic colitis	895:967	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	10	45	theme	normal	1144:1149	arg1	feces					1151:1155	normal feces	1144:1155	normal feces	1144:1155	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	2	46	theme	microbial	195:203	arg1	ecosystem					205:213	the microbial ecosystem	191:213	the microbial ecosystem	191:213	Disruption of the microbial ecosystem is considered to be an important cause of IBD.
36176029	2	47	theme	ecosystem	205:213	arg1	Disruption					177:186	Disruption	177:186	Disruption of the microbial ecosystem	177:213	Disruption of the microbial ecosystem is considered to be an important cause of IBD.
36176029	2	47	theme	ecosystem	205:213	arg1	cause					248:252	an important cause	235:252	an important cause of IBD	235:259	Disruption of the microbial ecosystem is considered to be an important cause of IBD.
36176029	6	48	theme	dextran	638:644	arg1	sulfate					646:652	dextran sulfate sodium (DSS)	638:665	dextran sulfate sodium (DSS)	638:665	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	7	49	theme	Several	733:739	arg1	miRNAs					741:746	Several miRNAs	733:746	Several miRNAs	733:746	Several miRNAs were significantly altered in colitis EVs.
36176029	6	50	theme	colonic	707:713	arg1	EVs					715:717	colonic EVs	707:717	colonic EVs from colitis	707:730	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	6	51	from	colitis	724:730	arg1	EVs					715:717	colonic EVs	707:717	colonic EVs from colitis	707:730	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	6	51	from	colitis	724:730	arg1	changes					686:692	the changes	682:692	the changes of miRNAs in colonic EVs from colitis	682:730	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	10	52	theme	similar	1318:1324	arg1	effects					1326:1332	similar effects	1318:1332	similar effects	1318:1332	Notably, MVs from normal feces could effectively reverse the composition of the intestinal microbiota, restore the intestinal barrier and rescue colitis, and BMVs from colitis would also have similar effects after miR-200b-3p treatment.
36176029	3	53	from	participants	301:312	arg1	communication					345:357	cell-cell and cell-organism communication	317:357	cell-cell and cell-organism communication	317:357	Extracellular vesicles (EVs) are vital participants in cell-cell and cell-organism communication.
36176029	2	54	theme	important	238:246	arg1	Disruption					177:186	Disruption	177:186	Disruption of the microbial ecosystem	177:213	Disruption of the microbial ecosystem is considered to be an important cause of IBD.
36176029	2	54	theme	important	238:246	arg1	cause					248:252	an important cause	235:252	an important cause of IBD	235:259	Disruption of the microbial ecosystem is considered to be an important cause of IBD.
36176029	11	55	theme	host-microbe	1414:1425	arg1	cycle					1439:1443	a vesicle-based host-microbe interaction cycle	1398:1443	a vesicle-based host-microbe interaction cycle	1398:1443	Our results preliminarily identify a vesicle-based host-microbe interaction cycle in colitis and provide new ideas for colitis treatment.
36176029	4	56	link	bacteria-derived	386:401	arg1	OMVs					422:425	OMVs	422:425	OMVs	422:425	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	4	56	link	bacteria-derived	386:401	arg1	vesicles					412:419	bacteria-derived membrane vesicles	386:419	bacteria-derived membrane vesicles (OMVs)	386:426	Both host-derived EVs and bacteria-derived membrane vesicles (OMVs) contribute to homeostasis in the intestine.
36176029	8	57	theme	macrophage	832:841	arg1	polarization					843:854	M1 macrophage polarization	829:854	M1 macrophage polarization	829:854	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	5	58	theme	MVs	509:511	arg1	roles					485:489	the roles	481:489	the roles of EVs-miRNAs and MVs in host-microbe interactions in colitis	481:551	However, the roles of EVs-miRNAs and MVs in host-microbe interactions in colitis remain unclear.
36176029	8	59	theme	miR-181b-5p	791:801	arg1	transplantation					803:817	miR-181b-5p transplantation	791:817	miR-181b-5p transplantation	791:817	miR-181b-5p transplantation inhibited M1 macrophage polarization and promoted M2 polarization to reduce the levels of inflammation both in acute and remission of chronic colitis.
36176029	11	60	theme	vesicle-based	1400:1412	arg1	cycle					1439:1443	a vesicle-based host-microbe interaction cycle	1398:1443	a vesicle-based host-microbe interaction cycle	1398:1443	Our results preliminarily identify a vesicle-based host-microbe interaction cycle in colitis and provide new ideas for colitis treatment.
36176029	5	61	from	roles	485:489	arg1	interactions					529:540	host-microbe interactions	516:540	host-microbe interactions in colitis	516:551	However, the roles of EVs-miRNAs and MVs in host-microbe interactions in colitis remain unclear.
36176029	6	62	from	changes	686:692	arg1	EVs					715:717	colonic EVs	707:717	colonic EVs from colitis	707:730	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	6	62	from	changes	686:692	arg1	colitis					724:730	colitis	724:730	colitis	724:730	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36176029	6	63	theme	colitis	611:617	arg1	model					602:606	the animal model	591:606	the animal model of colitis	591:617	In the present study, the animal model of colitis was established by dextran sulfate sodium (DSS) to investigate the changes of miRNAs in colonic EVs from colitis.
36144825	7	0	theme	raw	1141:1143	arg1	material					1145:1152	the analyzed lignocellulosic raw material	1112:1152	the analyzed lignocellulosic raw material	1112:1152	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	6	1	theme	skeleton	1056:1063	arg1	lignins					971:977	lignins	971:977	lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	971:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	1	theme	skeleton	1056:1063	arg1	stretching					1069:1078	aromatic skeleton C-C stretching	1047:1078	aromatic skeleton C-C stretching	1047:1078	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	1	2	theme	ethanol	173:179	arg1	production					181:190	cellulosic ethanol production	162:190	cellulosic ethanol production	162:190	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	6	3	theme	asymmetric	1016:1025	arg1	lignins					971:977	lignins	971:977	lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	971:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	3	theme	asymmetric	1016:1025	arg1	bending					1027:1033	asymmetric bending	1016:1033	asymmetric bending in CH3	1016:1040	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	4	theme	groups	936:941	arg1	peaks					916:920	The peaks	912:920	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	912:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	5	from	bending	1027:1033	arg1	CH3					1038:1040	CH3	1038:1040	CH3	1038:1040	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	5	from	bending	1027:1033	arg1	ring					1010:1013	the syringyl ring	997:1013	the syringyl ring	997:1013	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	1	6	theme	production	181:190	arg1	efficiency					148:157	the efficiency	144:157	the efficiency of cellulosic ethanol production	144:190	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	8	7	theme	stillage	1458:1465	arg1	pretreatment					1436:1447	the pretreatment	1432:1447	the pretreatment of wheat stillage that did not require additional nutrient supplementation	1432:1522	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	8	theme	ethanol	1349:1355	arg1	g/L					1371:1373	23.57 ± 0.10 g/L	1358:1373	23.57 ± 0.10 g/L	1358:1373	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	8	theme	ethanol	1349:1355	arg1	concentration					1332:1344	The highest observed concentration	1311:1344	The highest observed concentration of ethanol	1311:1355	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	6	9	theme	functional	925:934	arg1	groups					936:941	functional groups	925:941	functional groups	925:941	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	2	10	theme	sodium	370:375	arg1	hydrotrope					402:411	sodium cumene sulphonate (NaCS) hydrotrope	370:411	sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol	370:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	6	11	from	stretching	1069:1078	arg1	CH3					1038:1040	CH3	1038:1040	CH3	1038:1040	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	11	from	stretching	1069:1078	arg1	ring					1010:1013	the syringyl ring	997:1013	the syringyl ring	997:1013	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	2	12	theme	microwave-assisted	301:318	arg1	pretreatment					320:331	microwave-assisted pretreatment	301:331	microwave-assisted pretreatment of wheat stillage	301:349	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	2	13	theme	pretreatment	320:331	arg1	effect					291:296	the effect	287:296	the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol	287:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	5	14	from	changes	846:852	arg1	composition					865:875	biomass composition	857:875	biomass composition	857:875	In post pretreatment, changes in biomass composition were also visible on FTIR spectra.
36144825	7	15	theme	lignocellulosic	1125:1139	arg1	material					1145:1152	the analyzed lignocellulosic raw material	1112:1152	the analyzed lignocellulosic raw material	1112:1152	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	2	16	theme	cumene	377:382	arg1	hydrotrope					402:411	sodium cumene sulphonate (NaCS) hydrotrope	370:411	sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol	370:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	3	17	theme	raw	600:602	arg1	material					604:611	the raw material	596:611	the raw material used	596:616	As a result of microwave pretreatment, the composition of the wheat stillage biomass changed significantly when compared with the raw material used, before treatment.
36144825	7	18	theme	glucose	1203:1209	arg1	conversion					1189:1198	the complete conversion	1176:1198	the complete conversion of glucose to ethanol	1176:1220	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	4	19	theme	dominant	758:765	arg1	component					767:775	the dominant component	754:775	the dominant component	754:775	Microwave-assisted pretreatment with NaCS effectively reduced the lignin content and hemicellulose, making cellulose the dominant component of biomass, which accounted for 42.91 ± 0.10%.
36144825	7	20	theme	complete	1180:1187	arg1	conversion					1189:1198	the complete conversion	1176:1198	the complete conversion of glucose to ethanol	1176:1220	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	3	21	theme	microwave	485:493	arg1	pretreatment					495:506	microwave pretreatment	485:506	microwave pretreatment	485:506	As a result of microwave pretreatment, the composition of the wheat stillage biomass changed significantly when compared with the raw material used, before treatment.
36144825	3	22	theme	biomass	547:553	arg1	composition					513:523	the composition	509:523	the composition of the wheat stillage biomass	509:553	As a result of microwave pretreatment, the composition of the wheat stillage biomass changed significantly when compared with the raw material used, before treatment.
36144825	5	23	theme	FTIR	898:901	arg1	spectra					903:909	FTIR spectra	898:909	FTIR spectra	898:909	In post pretreatment, changes in biomass composition were also visible on FTIR spectra.
36144825	4	24	theme	lignin	703:708	arg1	content					710:716	the lignin content	699:716	the lignin content	699:716	Microwave-assisted pretreatment with NaCS effectively reduced the lignin content and hemicellulose, making cellulose the dominant component of biomass, which accounted for 42.91 ± 0.10%.
36144825	6	25	theme	syringyl	1001:1008	arg1	ring					1010:1013	the syringyl ring	997:1013	the syringyl ring	997:1013	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	5	26	from	pretreatment	832:843	arg1	visible					887:893	visible	887:893	visible	887:893	In post pretreatment, changes in biomass composition were also visible on FTIR spectra.
36144825	8	27	theme	highest	1315:1321	arg1	g/L					1371:1373	23.57 ± 0.10 g/L	1358:1373	23.57 ± 0.10 g/L	1358:1373	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	27	theme	highest	1315:1321	arg1	concentration					1332:1344	The highest observed concentration	1311:1344	The highest observed concentration of ethanol	1311:1355	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	28	theme	±	1364:1364	arg1	g/L					1371:1373	23.57 ± 0.10 g/L	1358:1373	23.57 ± 0.10 g/L	1358:1373	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	28	theme	±	1364:1364	arg1	concentration					1332:1344	The highest observed concentration	1311:1344	The highest observed concentration of ethanol	1311:1355	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	2	29	theme	hydrotrope	402:411	arg1	presence					358:365	the presence	354:365	the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol	354:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	0	30	theme	Ethanol	11:17	arg1	Production					19:28	Cellulosic Ethanol Production	0:28	Cellulosic Ethanol Production	0:28	Cellulosic Ethanol Production Using Waste Wheat Stillage after Microwave-Assisted Hydrotropic Pretreatment.
36144825	3	31	theme	stillage	538:545	arg1	biomass					547:553	the wheat stillage biomass	528:553	the wheat stillage biomass	528:553	As a result of microwave pretreatment, the composition of the wheat stillage biomass changed significantly when compared with the raw material used, before treatment.
36144825	2	32	theme	stillage	342:349	arg1	pretreatment					320:331	microwave-assisted pretreatment	301:331	microwave-assisted pretreatment of wheat stillage	301:349	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	1	33	theme	effective	199:207	arg1	elements					123:130	the key elements	115:130	the key elements influencing the efficiency of cellulosic ethanol production	115:190	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	1	33	theme	effective	199:207	arg1	pretreatment					209:220	the effective pretreatment	195:220	the effective pretreatment of lignocellulosic biomass	195:247	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	1	33	theme	effective	199:207	arg1	One					108:110	One	108:110	One	108:110	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	0	34	theme	Cellulosic	0:9	arg1	Production					19:28	Cellulosic Ethanol Production	0:28	Cellulosic Ethanol Production	0:28	Cellulosic Ethanol Production Using Waste Wheat Stillage after Microwave-Assisted Hydrotropic Pretreatment.
36144825	2	35	theme	wheat	336:340	arg1	stillage					342:349	wheat stillage	336:349	wheat stillage	336:349	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	8	36	theme	observed	1323:1330	arg1	g/L					1371:1373	23.57 ± 0.10 g/L	1358:1373	23.57 ± 0.10 g/L	1358:1373	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	36	theme	observed	1323:1330	arg1	concentration					1332:1344	The highest observed concentration	1311:1344	The highest observed concentration of ethanol	1311:1355	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	2	37	theme	NaCS	396:399	arg1	hydrotrope					402:411	sodium cumene sulphonate (NaCS) hydrotrope	370:411	sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol	370:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	2	38	theme	bioethanol	458:467	arg1	production					426:435	the production	422:435	the production of second-generation bioethanol	422:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	4	39	theme	biomass	780:786	arg1	component					767:775	the dominant component	754:775	the dominant component	754:775	Microwave-assisted pretreatment with NaCS effectively reduced the lignin content and hemicellulose, making cellulose the dominant component of biomass, which accounted for 42.91 ± 0.10%.
36144825	6	40	from	vibration	984:992	arg1	CH3					1038:1040	CH3	1038:1040	CH3	1038:1040	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	40	from	vibration	984:992	arg1	ring					1010:1013	the syringyl ring	997:1013	the syringyl ring	997:1013	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	7	41	from	yield	1254:1258	arg1	relation					1264:1271	relation	1264:1271	relation to the theoretical one	1264:1294	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	7	42	with	pretreatment	1096:1107	arg1	NaCS					1159:1162	NaCS	1159:1162	NaCS	1159:1162	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	5	43	theme	biomass	857:863	arg1	composition					865:875	biomass composition	857:875	biomass composition	857:875	In post pretreatment, changes in biomass composition were also visible on FTIR spectra.
36144825	2	44	theme	second-generation	440:456	arg1	bioethanol					458:467	second-generation bioethanol	440:467	second-generation bioethanol	440:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	4	45	theme	0.10	817:820	arg1	±					815:815	±	815:815	±	815:815	Microwave-assisted pretreatment with NaCS effectively reduced the lignin content and hemicellulose, making cellulose the dominant component of biomass, which accounted for 42.91 ± 0.10%.
36144825	1	46	theme	key	119:121	arg1	elements					123:130	the key elements	115:130	the key elements influencing the efficiency of cellulosic ethanol production	115:190	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	1	47	theme	lignocellulosic	225:239	arg1	biomass					241:247	lignocellulosic biomass	225:247	lignocellulosic biomass	225:247	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	0	48	theme	Wheat	42:46	arg1	Stillage					48:55	Waste Wheat Stillage	36:55	Waste Wheat Stillage	36:55	Cellulosic Ethanol Production Using Waste Wheat Stillage after Microwave-Assisted Hydrotropic Pretreatment.
36144825	6	49	theme	C-O	980:982	arg1	lignins					971:977	lignins	971:977	lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	971:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	49	theme	C-O	980:982	arg1	vibration					984:992	C-O vibration	980:992	C-O vibration in the syringyl ring	980:1013	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	50	theme	aromatic	1047:1054	arg1	lignins					971:977	lignins	971:977	lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	971:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	50	theme	aromatic	1047:1054	arg1	stretching					1069:1078	aromatic skeleton C-C stretching	1047:1078	aromatic skeleton C-C stretching	1047:1078	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	4	51	with	pretreatment	656:667	arg1	NaCS					674:677	NaCS	674:677	NaCS	674:677	Microwave-assisted pretreatment with NaCS effectively reduced the lignin content and hemicellulose, making cellulose the dominant component of biomass, which accounted for 42.91 ± 0.10%.
36144825	1	52	theme	biomass	241:247	arg1	elements					123:130	the key elements	115:130	the key elements influencing the efficiency of cellulosic ethanol production	115:190	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	1	52	theme	biomass	241:247	arg1	pretreatment					209:220	the effective pretreatment	195:220	the effective pretreatment of lignocellulosic biomass	195:247	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	1	52	theme	biomass	241:247	arg1	One					108:110	One	108:110	One	108:110	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	0	53	theme	Waste	36:40	arg1	Stillage					48:55	Waste Wheat Stillage	36:55	Waste Wheat Stillage	36:55	Cellulosic Ethanol Production Using Waste Wheat Stillage after Microwave-Assisted Hydrotropic Pretreatment.
36144825	8	54	theme	method	1416:1421	arg1	effectiveness					1395:1407	the high effectiveness	1386:1407	the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation	1386:1522	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	55	theme	23.57	1358:1362	arg1	g/L					1371:1373	23.57 ± 0.10 g/L	1358:1373	23.57 ± 0.10 g/L	1358:1373	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	8	55	theme	23.57	1358:1362	arg1	concentration					1332:1344	The highest observed concentration	1311:1344	The highest observed concentration of ethanol	1311:1355	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	7	56	theme	theoretical	1280:1290	arg1	one					1292:1294	the theoretical one	1276:1294	the theoretical one	1276:1294	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	7	57	theme	material	1145:1152	arg1	pretreatment					1096:1107	The pretreatment	1092:1107	The pretreatment of the analyzed lignocellulosic raw material with NaCS	1092:1162	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	6	58	theme	lignins	971:977	arg1	characteristic					953:966	characteristic	953:966	characteristic	953:966	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	59	dep	lignins	971:977	arg1	lignins					971:977	lignins	971:977	lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	971:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	59	dep	lignins	971:977	arg1	vibration					984:992	C-O vibration	980:992	C-O vibration in the syringyl ring	980:1013	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	59	dep	lignins	971:977	arg1	stretching					1069:1078	aromatic skeleton C-C stretching	1047:1078	aromatic skeleton C-C stretching	1047:1078	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	59	dep	lignins	971:977	arg1	bending					1027:1033	asymmetric bending	1016:1033	asymmetric bending in CH3	1016:1040	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	8	60	theme	additional	1488:1497	arg1	supplementation					1508:1522	additional nutrient supplementation	1488:1522	additional nutrient supplementation	1488:1522	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	1	61	theme	elements	123:130	arg1	elements					123:130	the key elements	115:130	the key elements influencing the efficiency of cellulosic ethanol production	115:190	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	1	61	theme	elements	123:130	arg1	pretreatment					209:220	the effective pretreatment	195:220	the effective pretreatment of lignocellulosic biomass	195:247	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	1	61	theme	elements	123:130	arg1	One					108:110	One	108:110	One	108:110	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	5	62	theme	post	827:830	arg1	pretreatment					832:843	post pretreatment	827:843	post pretreatment	827:843	In post pretreatment, changes in biomass composition were also visible on FTIR spectra.
36144825	8	63	theme	wheat	1452:1456	arg1	stillage					1458:1465	wheat stillage	1452:1465	wheat stillage that did not require additional nutrient supplementation	1452:1522	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	7	64	theme	analyzed	1116:1123	arg1	material					1145:1152	the analyzed lignocellulosic raw material	1112:1152	the analyzed lignocellulosic raw material	1112:1152	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	2	65	from	effect	291:296	arg1	presence					358:365	the presence	354:365	the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol	354:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	6	66	theme	characteristic	953:966	arg1	bonds					947:951	bonds	947:951	bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	947:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	3	67	theme	pretreatment	495:506	arg1	result					475:480	a result	473:480	a result of microwave pretreatment	473:506	As a result of microwave pretreatment, the composition of the wheat stillage biomass changed significantly when compared with the raw material used, before treatment.
36144825	4	68	theme	42.91	809:813	arg1	±					815:815	±	815:815	±	815:815	Microwave-assisted pretreatment with NaCS effectively reduced the lignin content and hemicellulose, making cellulose the dominant component of biomass, which accounted for 42.91 ± 0.10%.
36144825	1	69	theme	cellulosic	162:171	arg1	production					181:190	cellulosic ethanol production	162:190	cellulosic ethanol production	162:190	One of the key elements influencing the efficiency of cellulosic ethanol production is the effective pretreatment of lignocellulosic biomass.
36144825	8	70	theme	nutrient	1499:1506	arg1	supplementation					1508:1522	additional nutrient supplementation	1488:1522	additional nutrient supplementation	1488:1522	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	0	71	theme	Hydrotropic	82:92	arg1	Pretreatment					94:105	Microwave-Assisted Hydrotropic Pretreatment	63:105	Microwave-Assisted Hydrotropic Pretreatment	63:105	Cellulosic Ethanol Production Using Waste Wheat Stillage after Microwave-Assisted Hydrotropic Pretreatment.
36144825	8	72	theme	high	1390:1393	arg1	effectiveness					1395:1407	the high effectiveness	1386:1407	the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation	1386:1522	The highest observed concentration of ethanol, 23.57 ± 0.10 g/L, indicated the high effectiveness of the method used for the pretreatment of wheat stillage that did not require additional nutrient supplementation.
36144825	6	73	theme	C-C	1065:1067	arg1	lignins					971:977	lignins	971:977	lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	971:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	6	73	theme	C-C	1065:1067	arg1	stretching					1069:1078	aromatic skeleton C-C stretching	1047:1078	aromatic skeleton C-C stretching	1047:1078	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	3	74	theme	wheat	532:536	arg1	biomass					547:553	the wheat stillage biomass	528:553	the wheat stillage biomass	528:553	As a result of microwave pretreatment, the composition of the wheat stillage biomass changed significantly when compared with the raw material used, before treatment.
36144825	7	75	theme	%	1308:1308	arg1	yield					1254:1258	yield	1254:1258	yield (in relation to the theoretical one) of above 91%	1254:1308	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	0	76	theme	Microwave-Assisted	63:80	arg1	Pretreatment					94:105	Microwave-Assisted Hydrotropic Pretreatment	63:105	Microwave-Assisted Hydrotropic Pretreatment	63:105	Cellulosic Ethanol Production Using Waste Wheat Stillage after Microwave-Assisted Hydrotropic Pretreatment.
36144825	6	77	theme	bonds	947:951	arg1	peaks					916:920	The peaks	912:920	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching)	912:1079	The peaks of functional groups and bonds characteristic of lignins (C-O vibration in the syringyl ring, asymmetric bending in CH3, and aromatic skeleton C-C stretching) decreased.
36144825	2	78	theme	study	265:269	arg1	aim					254:256	The aim	250:256	The aim of the study	250:269	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	5	79	from	visible	887:893	arg1	pretreatment					832:843	post pretreatment	827:843	post pretreatment	827:843	In post pretreatment, changes in biomass composition were also visible on FTIR spectra.
36144825	2	80	theme	sulphonate	384:393	arg1	hydrotrope					402:411	sodium cumene sulphonate (NaCS) hydrotrope	370:411	sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol	370:467	The aim of the study was to evaluate the effect of microwave-assisted pretreatment of wheat stillage in the presence of sodium cumene sulphonate (NaCS) hydrotrope used for the production of second-generation bioethanol.
36144825	7	81	theme	process	1240:1246	arg1	h					1231:1231	48 h	1228:1231	48 h of the process	1228:1246	The pretreatment of the analyzed lignocellulosic raw material with NaCS resulted in the complete conversion of glucose to ethanol after 48 h of the process, with yield (in relation to the theoretical one) of above 91%.
36144825	4	82	theme	Microwave-assisted	637:654	arg1	pretreatment					656:667	Microwave-assisted pretreatment	637:667	Microwave-assisted pretreatment with NaCS	637:677	Microwave-assisted pretreatment with NaCS effectively reduced the lignin content and hemicellulose, making cellulose the dominant component of biomass, which accounted for 42.91 ± 0.10%.
35931091	2	0	theme	treatments	711:720	arg1	treatments					711:720	the following inoculant treatments	687:720	the following inoculant treatments	687:720	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	0	theme	treatments	711:720	arg1	one					680:682	one	680:682	one	680:682	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	1	theme	DSM	769:771	arg1	12856					773:777	Lentilactobacillus buchneri DSM 12856	741:777	Lentilactobacillus buchneri DSM 12856	741:777	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	5	2	theme	=	1334:1334	arg1	P					1332:1332	P = 0.001	1332:1340	P = 0.001	1332:1340	Concentrations of total acids were greater (P = 0.001) in HMC65 than HMC70 and greater (P = 0.001) in HMC treated with LBLP and LBLD than CON.
35931091	1	3	theme	inoculation	244:254	arg1	effect					224:229	the effect	220:229	the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations	220:408	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	12	4	theme	fermentability	2385:2398	arg1	determinant					2366:2376	a primary determinant	2356:2376	a primary determinant of HMC fermentability	2356:2398	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	2	5	theme	following	691:699	arg1	treatments					711:720	the following inoculant treatments	687:720	the following inoculant treatments	687:720	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	6	theme	Lentilactobacillus	741:758	arg1	12856					773:777	Lentilactobacillus buchneri DSM 12856	741:777	Lentilactobacillus buchneri DSM 12856	741:777	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	5	7	theme	total	1306:1310	arg1	acids					1312:1316	total acids	1306:1316	total acids	1306:1316	Concentrations of total acids were greater (P = 0.001) in HMC65 than HMC70 and greater (P = 0.001) in HMC treated with LBLP and LBLD than CON.
35931091	1	8	theme	storage	260:266	arg1	length					268:273	storage length	260:273	storage length	260:273	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	4	9	theme	three-way	1098:1106	arg1	interaction					1108:1118	A three-way interaction	1096:1118	A three-way interaction	1096:1118	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	12	10	theme	primary	2358:2364	arg1	determinant					2366:2376	a primary determinant	2356:2376	a primary determinant of HMC fermentability	2356:2398	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	2	11	theme	quadruplicate	626:638	arg1	pouches					647:653	quadruplicate vacuum pouches	626:653	quadruplicate vacuum pouches untreated (CON)	626:669	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	6	12	theme	acetic	1560:1565	arg1	acid					1567:1570	acetic acid	1560:1570	acetic acid	1560:1570	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for concentrations of acetic acid.
35931091	12	13	theme	DM	2319:2320	arg1	concentration					2322:2334	DM concentration	2319:2334	DM concentration at ensiling	2319:2346	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	12	14	theme	acid	2304:2307	arg1	production					2272:2281	the production	2268:2281	the production of antifungal acetic acid	2268:2307	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	0	15	theme	corn	116:119	arg1	profile					71:77	fermentation profile	58:77	fermentation profile	58:77	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	0	15	theme	corn	116:119	arg1	value					93:97	nutritive value	83:97	nutritive value	83:97	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	1	16	theme	experiment	193:202	arg1	objective					175:183	The objective	171:183	The objective of this experiment	171:202	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	11	17	theme	small	2143:2147	arg1	differences					2149:2159	small differences	2143:2159	small differences among inoculants	2143:2176	StarchD was also generally greater for HMC65 treatments compared to HMC70, with small differences among inoculants.
35931091	1	18	theme	dry	379:381	arg1	DM					391:392	DM	391:392	DM	391:392	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	1	18	theme	dry	379:381	arg1	matter					383:388	dry matter	379:388	2 different dry matter (DM) concentrations	367:408	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	2	19	theme	%	539:539	arg1	as-fed					495:500	as-fed	495:500	as-fed	495:500	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	19	theme	%	539:539	arg1	DM					541:542	HMC65; 67.6% DM	528:542	HMC65; 67.6% DM	528:542	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	9	20	theme	DM	1823:1824	arg1	concentration					1826:1838	DM concentration	1823:1838	DM concentration	1823:1838	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for 7-h starch disappearance (starchD).
35931091	0	21	theme	different	134:142	arg1	concentrations					155:168	2 different dry matter concentrations	132:168	2 different dry matter concentrations	132:168	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	2	22	theme	×	888:888	arg1	cfu					894:896	3.0 × 105 cfu	884:896	3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	884:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	4	23	contain	had	1250:1252	arg1	treatments					1229:1238	HMC65 treatments	1223:1238	HMC65 treatments	1223:1238	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	4	23	contain	had	1250:1252	arg2	pH					1262:1263	a lower pH	1254:1263	a lower pH	1254:1263	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	2	24	theme	%	599:599	arg1	DM					584:585	approximately 70% DM	566:585	approximately 70% DM (HMC70; 71.0% DM)	566:603	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	24	theme	%	599:599	arg1	DM					601:602	HMC70; 71.0% DM	588:602	HMC70; 71.0% DM	588:602	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	0	25	theme	matter	148:153	arg1	concentrations					155:168	2 different dry matter concentrations	132:168	2 different dry matter concentrations	132:168	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	10	26	theme	inoculant	1979:1987	arg1	combinations					1999:2010	all DM concentration and inoculant treatment combinations	1954:2010	combinations	1999:2010	Across all DM concentration and inoculant treatment combinations, starchD increased with increasing storage length.
35931091	6	27	theme	storage	1499:1505	arg1	length					1507:1512	storage length	1499:1512	storage length	1499:1512	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for concentrations of acetic acid.
35931091	2	28	theme	DSM	850:852	arg1	16243					854:858	Pediococcus acidilactici DSM 16243	825:858	Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	825:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	1	29	theme	corn	345:348	arg1	profile					295:301	fermentation profile	282:301	fermentation profile	282:301	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	1	29	theme	corn	345:348	arg1	composition					316:326	nutrient composition	307:326	nutrient composition	307:326	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	2	30	dep	DM	584:585	arg1	%					582:582	%	582:582	%	582:582	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	0	31	from	Effect	0:5	arg1	profile					71:77	fermentation profile	58:77	fermentation profile	58:77	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	0	31	from	Effect	0:5	arg1	value					93:97	nutritive value	83:97	nutritive value	83:97	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	0	32	theme	nutritive	83:91	arg1	value					93:97	nutritive value	83:97	nutritive value	83:97	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	7	33	theme	acid	1589:1592	arg1	concentrations					1594:1607	acetic acid concentrations	1582:1607	acetic acid concentrations	1582:1607	At 14 d, acetic acid concentrations were greater in HMC65 treated with LBLD than other treatments.
35931091	2	34	theme	DSM	971:973	arg1	32074					975:979	Lentilactobacillus diolivorans DSM 32074	940:979	Lentilactobacillus diolivorans DSM 32074	940:979	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	12	35	from	ensiling	2339:2346	arg1	concentration					2322:2334	DM concentration	2319:2334	DM concentration at ensiling	2319:2346	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	10	36	theme	storage	2047:2053	arg1	length					2055:2060	storage length	2047:2060	storage length	2047:2060	Across all DM concentration and inoculant treatment combinations, starchD increased with increasing storage length.
35931091	2	37	theme	DSM	1002:1004	arg1	16243					1006:1010	P. acidilactici DSM 16243	986:1010	P. acidilactici DSM 16243	986:1010	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	38	dep	Lentilactobacillus	940:957	arg1	diolivorans					959:969	diolivorans	959:969	diolivorans	959:969	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	1	39	theme	length	268:273	arg1	effect					224:229	the effect	220:229	the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations	220:408	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	0	40	dep	profile	71:77	arg1	the					54:56	the	54:56	the	54:56	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	8	41	theme	acetic	1709:1714	arg1	acid					1716:1719	acetic acid	1709:1719	acetic acid	1709:1719	Likewise, at 56 d, concentrations of acetic acid were greatest in HMC65 treated with LBLD, followed by HMC70 treated with LBLD.
35931091	2	42	theme	High-moisture	411:423	arg1	corn					425:428	High-moisture corn	411:428	High-moisture corn	411:428	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	43	theme	Lactiplantibacillus	780:798	arg1	12836					814:818	Lactiplantibacillus plantarum DSM 12836	780:818	Lactiplantibacillus plantarum DSM 12836	780:818	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	44	theme	P.	986:987	arg1	16243					1006:1010	P. acidilactici DSM 16243	986:1010	P. acidilactici DSM 16243	986:1010	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	4	45	theme	storage	1203:1209	arg1	lengths					1211:1217	storage lengths	1203:1217	storage lengths	1203:1217	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	0	46	theme	microbial	10:18	arg1	inoculation					20:30	microbial inoculation	10:30	microbial inoculation	10:30	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	2	47	theme	DM	456:457	arg1	concentrations					459:472	kernel DM concentrations	449:472	kernel DM concentrations	449:472	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	48	theme	12856	773:777	arg1	cfu					734:736	105 cfu	730:736	one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	680:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	5	49	theme	=	1378:1378	arg1	HMC70					1357:1361	HMC70	1357:1361	HMC70	1357:1361	Concentrations of total acids were greater (P = 0.001) in HMC65 than HMC70 and greater (P = 0.001) in HMC treated with LBLP and LBLD than CON.
35931091	5	49	theme	=	1378:1378	arg1	P					1376:1376	P = 0.001	1376:1384	P = 0.001	1376:1384	Concentrations of total acids were greater (P = 0.001) in HMC65 than HMC70 and greater (P = 0.001) in HMC treated with LBLP and LBLD than CON.
35931091	6	50	theme	DM	1454:1455	arg1	concentration					1457:1469	DM concentration	1454:1469	DM concentration	1454:1469	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for concentrations of acetic acid.
35931091	9	51	theme	starch	1915:1920	arg1	starchD					1937:1943	starchD	1937:1943	starchD	1937:1943	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for 7-h starch disappearance (starchD).
35931091	9	51	theme	starch	1915:1920	arg1	disappearance					1922:1934	7-h starch disappearance	1911:1934	7-h starch disappearance (starchD)	1911:1944	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for 7-h starch disappearance (starchD).
35931091	0	52	theme	length	44:49	arg1	Effect					0:5	Effect	0:5	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn	0:119	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	1	53	theme	nutrient	307:314	arg1	composition					316:326	nutrient composition	307:326	nutrient composition	307:326	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	2	54	theme	DSM	810:812	arg1	12836					814:818	Lactiplantibacillus plantarum DSM 12836	780:818	Lactiplantibacillus plantarum DSM 12836	780:818	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	55	theme	Lentilactobacillus	940:957	arg1	32074					975:979	Lentilactobacillus diolivorans DSM 32074	940:979	Lentilactobacillus diolivorans DSM 32074	940:979	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	9	56	theme	7-h	1911:1913	arg1	starchD					1937:1943	starchD	1937:1943	starchD	1937:1943	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for 7-h starch disappearance (starchD).
35931091	9	56	theme	7-h	1911:1913	arg1	disappearance					1922:1934	7-h starch disappearance	1911:1934	7-h starch disappearance (starchD)	1911:1944	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for 7-h starch disappearance (starchD).
35931091	2	57	theme	12856	933:937	arg1	g					864:864	g	864:864	g of HMC (LBLP)	864:878	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	57	theme	12856	933:937	arg1	cfu					894:896	3.0 × 105 cfu	884:896	3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	884:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	4	58	theme	lower	1256:1260	arg1	pH					1262:1263	a lower pH	1254:1263	a lower pH	1254:1263	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	2	59	dep	untreated	655:663	arg1	CON					666:668	CON	666:668	CON	666:668	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	1	60	theme	microbial	234:242	arg1	inoculation					244:254	microbial inoculation	234:254	microbial inoculation	234:254	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	2	61	theme	inoculant	701:709	arg1	treatments					711:720	the following inoculant treatments	687:720	the following inoculant treatments	687:720	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	12	62	theme	HMC	2381:2383	arg1	fermentability					2385:2398	HMC fermentability	2381:2398	HMC fermentability	2381:2398	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	2	63	theme	untreated	655:663	arg1	pouches					647:653	quadruplicate vacuum pouches	626:653	quadruplicate vacuum pouches untreated (CON)	626:669	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	5	64	theme	acids	1312:1316	arg1	Concentrations					1288:1301	Concentrations	1288:1301	Concentrations of total acids	1288:1316	Concentrations of total acids were greater (P = 0.001) in HMC65 than HMC70 and greater (P = 0.001) in HMC treated with LBLP and LBLD than CON.
35931091	8	65	dep	greatest	1726:1733	arg1	followed					1763:1770	followed	1763:1770	followed by HMC70 treated with LBLD	1763:1797	Likewise, at 56 d, concentrations of acetic acid were greatest in HMC65 treated with LBLD, followed by HMC70 treated with LBLD.
35931091	2	66	dep	one	680:682	arg1	cfu					734:736	105 cfu	730:736	one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	680:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	67	theme	vacuum	640:645	arg1	pouches					647:653	quadruplicate vacuum pouches	626:653	quadruplicate vacuum pouches untreated (CON)	626:669	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	6	68	theme	acid	1567:1570	arg1	concentrations					1542:1555	concentrations	1542:1555	concentrations of acetic acid	1542:1570	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for concentrations of acetic acid.
35931091	7	69	theme	other	1654:1658	arg1	treatments					1660:1669	other treatments	1654:1669	other treatments	1654:1669	At 14 d, acetic acid concentrations were greater in HMC65 treated with LBLD than other treatments.
35931091	3	70	theme	Vacuum	1033:1038	arg1	pouches					1040:1046	Vacuum pouches	1033:1046	Vacuum pouches	1033:1046	Vacuum pouches were allowed to ferment for 7, 14, 28, or 56 d.
35931091	1	71	theme	different	369:377	arg1	concentrations					395:408	2 different dry matter (DM) concentrations	367:408	2 different dry matter (DM) concentrations	367:408	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	9	72	theme	storage	1868:1874	arg1	length					1876:1881	storage length	1868:1881	storage length	1868:1881	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for 7-h starch disappearance (starchD).
35931091	1	73	from	effect	224:229	arg1	profile					295:301	fermentation profile	282:301	fermentation profile	282:301	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	1	73	from	effect	224:229	arg1	composition					316:326	nutrient composition	307:326	nutrient composition	307:326	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	1	74	theme	matter	383:388	arg1	concentrations					395:408	2 different dry matter (DM) concentrations	367:408	2 different dry matter (DM) concentrations	367:408	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	2	75	theme	Pediococcus	825:835	arg1	16243					854:858	Pediococcus acidilactici DSM 16243	825:858	Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	825:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	12	76	theme	acetic	2297:2302	arg1	acid					2304:2307	antifungal acetic acid	2286:2307	antifungal acetic acid	2286:2307	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	12	77	theme	antifungal	2286:2295	arg1	acid					2304:2307	antifungal acetic acid	2286:2307	antifungal acetic acid	2286:2307	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	12	78	theme	HMC	2250:2252	arg1	fermentation					2234:2245	fermentation	2234:2245	fermentation of HMC	2234:2252	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	2	79	theme	16243	1006:1010	arg1	g					864:864	g	864:864	g of HMC (LBLP)	864:878	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	79	theme	16243	1006:1010	arg1	cfu					894:896	3.0 × 105 cfu	884:896	3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	884:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	9	80	theme	microbial	1841:1849	arg1	inoculation					1851:1861	microbial inoculation	1841:1861	microbial inoculation	1841:1861	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for 7-h starch disappearance (starchD).
35931091	0	81	theme	fermentation	58:69	arg1	profile					71:77	fermentation profile	58:77	fermentation profile	58:77	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	2	82	theme	16243	854:858	arg1	cfu					734:736	105 cfu	730:736	one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	680:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	1	83	theme	high-moisture	331:343	arg1	corn					345:348	high-moisture corn	331:348	high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations	331:408	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	1	83	theme	high-moisture	331:343	arg1	HMC					351:353	HMC	351:353	HMC	351:353	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	10	84	theme	DM	1958:1959	arg1	concentration					1961:1973	all DM concentration and inoculant treatment combinations	1954:2010	concentration	1961:1973	Across all DM concentration and inoculant treatment combinations, starchD increased with increasing storage length.
35931091	2	85	theme	acidilactici	837:848	arg1	16243					854:858	Pediococcus acidilactici DSM 16243	825:858	Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	825:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	86	theme	HMC	1021:1023	arg1	g					1016:1016	g	1016:1016	g of HMC (LBLD)	1016:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	4	87	theme	HMC	1158:1160	arg1	pH					1152:1153	the pH	1148:1153	the pH	1148:1153	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	7	88	theme	acetic	1582:1587	arg1	acid					1589:1592	acetic acid	1582:1592	acetic acid concentrations	1582:1607	At 14 d, acetic acid concentrations were greater in HMC65 treated with LBLD than other treatments.
35931091	2	89	dep	%	582:582	arg1	70					580:581	70	580:581	70	580:581	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	90	theme	HMC	869:871	arg1	g					864:864	g	864:864	g of HMC (LBLP)	864:878	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	90	theme	HMC	869:871	arg1	cfu					894:896	3.0 × 105 cfu	884:896	3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	884:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	91	theme	32074	975:979	arg1	g					864:864	g	864:864	g of HMC (LBLP)	864:878	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	91	theme	32074	975:979	arg1	cfu					894:896	3.0 × 105 cfu	884:896	3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	884:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	0	92	theme	high-moisture	102:114	arg1	corn					116:119	high-moisture corn	102:119	high-moisture corn	102:119	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	10	93	theme	treatment	1989:1997	arg1	combinations					1999:2010	all DM concentration and inoculant treatment combinations	1954:2010	combinations	1999:2010	Across all DM concentration and inoculant treatment combinations, starchD increased with increasing storage length.
35931091	1	94	dep	profile	295:301	arg1	the					278:280	the	278:280	the	278:280	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	4	95	theme	HMC65	1223:1227	arg1	treatments					1229:1238	HMC65 treatments	1223:1238	HMC65 treatments	1223:1238	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	8	96	theme	acid	1716:1719	arg1	concentrations					1691:1704	concentrations	1691:1704	concentrations of acetic acid	1691:1719	Likewise, at 56 d, concentrations of acetic acid were greatest in HMC65 treated with LBLD, followed by HMC70 treated with LBLD.
35931091	2	97	theme	plantarum	800:808	arg1	12836					814:818	Lactiplantibacillus plantarum DSM 12836	780:818	Lactiplantibacillus plantarum DSM 12836	780:818	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	12	98	theme	microbial	2200:2208	arg1	inoculation					2210:2220	microbial inoculation	2200:2220	microbial inoculation	2200:2220	Results suggest that microbial inoculation can improve fermentation of HMC by increasing the production of antifungal acetic acid, but that DM concentration at ensiling remains a primary determinant of HMC fermentability.
35931091	2	99	theme	acidilactici	989:1000	arg1	16243					1006:1010	P. acidilactici DSM 16243	986:1010	P. acidilactici DSM 16243	986:1010	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	0	100	theme	inoculation	20:30	arg1	Effect					0:5	Effect	0:5	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn	0:119	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	6	101	theme	microbial	1472:1480	arg1	inoculation					1482:1492	microbial inoculation	1472:1492	microbial inoculation	1472:1492	An interaction between DM concentration, microbial inoculation, and storage length was observed (P = 0.05) for concentrations of acetic acid.
35931091	2	102	dep	Lentilactobacillus	741:758	arg1	buchneri					760:767	buchneri	760:767	buchneri	760:767	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	0	103	theme	dry	144:146	arg1	concentrations					155:168	2 different dry matter concentrations	132:168	2 different dry matter concentrations	132:168	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	0	104	theme	storage	36:42	arg1	length					44:49	storage length	36:49	storage length	36:49	Effect of microbial inoculation and storage length on the fermentation profile and nutritive value of high-moisture corn ensiled at 2 different dry matter concentrations.
35931091	1	105	theme	fermentation	282:293	arg1	profile					295:301	fermentation profile	282:301	fermentation profile	282:301	The objective of this experiment was to evaluate the effect of microbial inoculation and storage length on the fermentation profile and nutrient composition of high-moisture corn (HMC) ensiled at 2 different dry matter (DM) concentrations.
35931091	5	106	dep	greater	1323:1329	arg1	P					1332:1332	P = 0.001	1332:1340	P = 0.001	1332:1340	Concentrations of total acids were greater (P = 0.001) in HMC65 than HMC70 and greater (P = 0.001) in HMC treated with LBLP and LBLD than CON.
35931091	2	107	theme	kernel	449:454	arg1	concentrations					459:472	kernel DM concentrations	449:472	kernel DM concentrations	449:472	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	108	theme	Lentilactobacillus	901:918	arg1	12856					933:937	Lentilactobacillus buchneri DSM 12856	901:937	Lentilactobacillus buchneri DSM 12856	901:937	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	109	dep	Lentilactobacillus	901:918	arg1	buchneri					920:927	buchneri	920:927	buchneri	920:927	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	11	110	theme	HMC65	2102:2106	arg1	treatments					2108:2117	HMC65 treatments	2102:2117	HMC65 treatments	2102:2117	StarchD was also generally greater for HMC65 treatments compared to HMC70, with small differences among inoculants.
35931091	2	111	theme	12836	814:818	arg1	cfu					734:736	105 cfu	730:736	one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	680:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	4	112	theme	other	1270:1274	arg1	treatments					1276:1285	other treatments	1270:1285	other treatments	1270:1285	A three-way interaction was observed (P = 0.01) for the pH of HMC, where CON for HMC70 was greatest across storage lengths and HMC65 treatments generally had a lower pH than other treatments.
35931091	2	113	theme	×	728:728	arg1	cfu					734:736	105 cfu	730:736	one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD)	680:1030	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35931091	2	114	theme	DSM	929:931	arg1	12856					933:937	Lentilactobacillus buchneri DSM 12856	901:937	Lentilactobacillus buchneri DSM 12856	901:937	High-moisture corn was harvested when kernel DM concentrations were approaching 65% as-fed, and either left undried (HMC65; 67.6% DM) or dried at 40 °C to approximately 70% DM (HMC70; 71.0% DM), and then ensiled in quadruplicate vacuum pouches untreated (CON) or after one of the following inoculant treatments: 6.36 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lactiplantibacillus plantarum DSM 12836, and Pediococcus acidilactici DSM 16243 per g of HMC (LBLP); or 3.0 × 105 cfu of Lentilactobacillus buchneri DSM 12856, Lentilactobacillus diolivorans DSM 32074, and P. acidilactici DSM 16243 per g of HMC (LBLD).
35216292	0	0	theme	Vesicle	99:105	arg1	Capacity					115:122	Different Extracellular Vesicle Release Capacity	75:122	Different Extracellular Vesicle Release Capacity	75:122	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	4	1	theme	CRC	648:650	arg1	organoids					668:676	CRC patient-derived organoids	648:676	CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue	648:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	2	2	theme	cases	347:351	arg1	cause					317:321	a leading cause	307:321	a leading cause of cancer-related death cases	307:351	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	1	3	theme	target	266:271	arg1	cells					273:277	target cells	266:277	target cells	266:277	Extracellular vesicles (EV) are released by virtually all cells and they transport biologically important molecules from the release site to target cells.
35216292	6	4	theme	CD44low	1030:1036	arg1	cell					1038:1041	CD44low cell	1030:1041	CD44low cell	1030:1041	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	5	5	theme	CD44high	950:957	arg1	cells					963:967	CD44high CRC cells	950:967	CD44high CRC cells	950:967	Interestingly, we detected an increased EV release by CD44high CRC cells.
35216292	4	6	theme	proliferation	841:853	arg1	intensity					855:863	a higher proliferation intensity	832:863	a higher proliferation intensity	832:863	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	6	7	dep	derived	1043:1049	arg1	cell					1038:1041	CD44low cell	1030:1041	CD44low cell	1030:1041	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	7	8	theme	fibroblasts	1244:1254	arg1	activation					1224:1233	activation	1224:1233	activation	1224:1233	We observed that EVs released by CD44high cells induced the proliferation and activation of colon fibroblasts more strongly than CD44low cells.
35216292	7	8	theme	fibroblasts	1244:1254	arg1	proliferation					1206:1218	proliferation	1206:1218	proliferation	1206:1218	We observed that EVs released by CD44high cells induced the proliferation and activation of colon fibroblasts more strongly than CD44low cells.
35216292	0	9	theme	Release	107:113	arg1	Capacity					115:122	Different Extracellular Vesicle Release Capacity	75:122	Different Extracellular Vesicle Release Capacity	75:122	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	4	10	theme	higher	834:839	arg1	intensity					855:863	a higher proliferation intensity	832:863	a higher proliferation intensity	832:863	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	6	11	theme	subpopulations	1130:1143	arg1	subpopulations					1130:1143	the above subpopulations	1120:1143	the above subpopulations	1120:1143	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	6	11	theme	subpopulations	1130:1143	arg1	one					1113:1115	one	1113:1115	one	1113:1115	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	3	12	theme	great	504:508	arg1	promise					510:516	a great promise	502:516	a great promise for tumor diagnostics	502:538	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	3	13	theme	releasing	463:471	arg1	cells					473:477	the releasing cells	459:477	the releasing cells	459:477	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	4	14	theme	cellular	696:703	arg1	heterogeneity					719:731	the cellular and molecular heterogeneity	692:731	the cellular and molecular heterogeneity of the original epithelial tumor tissue	692:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	4	15	theme	original	740:747	arg1	tissue					766:771	the original epithelial tumor tissue	736:771	the original epithelial tumor tissue	736:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	1	16	theme	all	179:181	arg1	cells					183:187	virtually all cells	169:187	virtually all cells	169:187	Extracellular vesicles (EV) are released by virtually all cells and they transport biologically important molecules from the release site to target cells.
35216292	9	17	contain	have	1511:1514	arg2	effect					1536:1541	a miRNA-independent effect	1516:1541	a miRNA-independent effect	1516:1541	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	9	17	contain	have	1511:1514	arg1	EVs					1507:1509	CRC cell-released EVs	1489:1509	CRC cell-released EVs	1489:1509	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	9	18	with	subpopulations	1414:1427	arg1	we					1474:1475	we	1474:1475	we	1474:1475	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	9	18	with	subpopulations	1414:1427	arg1	capabilities					1457:1468	different EV releasing capabilities	1434:1468	different EV releasing capabilities	1434:1468	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	9	19	theme	fibroblast	1546:1555	arg1	proliferation					1557:1569	fibroblast proliferation	1546:1569	fibroblast proliferation	1546:1569	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	2	20	theme	Colorectal	280:289	arg1	CRC					299:301	CRC	299:301	CRC	299:301	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	2	20	theme	Colorectal	280:289	arg1	cancer					291:296	Colorectal cancer	280:296	Colorectal cancer (CRC) is a leading cause of cancer-related death cases	280:351	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	7	21	theme	CD44low	1275:1281	arg1	cells					1283:1287	CD44low cells	1275:1287	CD44low cells	1275:1287	We observed that EVs released by CD44high cells induced the proliferation and activation of colon fibroblasts more strongly than CD44low cells.
35216292	0	22	theme	Expression	5:14	arg1	Intensity					16:24	CD44 Expression Intensity	0:24	CD44 Expression Intensity	0:24	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	6	23	theme	CD44high	1017:1024	arg1	cargos					1007:1012	the miRNA cargos	997:1012	the miRNA cargos of CD44high and CD44low cell derived EVs	997:1053	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	4	24	gly	heterogeneity	719:731	arg1	tissue					766:771	the original epithelial tumor tissue	736:771	the original epithelial tumor tissue	736:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	2	25	theme	leading	309:315	arg1	cause					317:321	a leading cause	307:321	a leading cause of cancer-related death cases	307:351	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	0	26	theme	CD44	0:3	arg1	Intensity					16:24	CD44 Expression Intensity	0:24	CD44 Expression Intensity	0:24	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	6	27	theme	above	1124:1128	arg1	subpopulations					1130:1143	the above subpopulations	1120:1143	the above subpopulations	1120:1143	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	4	28	theme	more	812:815	arg1	organoids					817:825	more organoids	812:825	more organoids	812:825	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	9	29	theme	CRC	1489:1491	arg1	EVs					1507:1509	CRC cell-released EVs	1489:1509	CRC cell-released EVs	1489:1509	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	3	30	theme	EV	409:410	arg1	cargo					412:416	the EV cargo	405:416	the EV cargo	405:416	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	5	31	theme	CRC	959:961	arg1	cells					963:967	CD44high CRC cells	950:967	CD44high CRC cells	950:967	Interestingly, we detected an increased EV release by CD44high CRC cells.
35216292	4	32	theme	CD44low	881:887	arg1	cells					889:893	CD44low cells	881:893	CD44low cells	881:893	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	7	33	theme	CD44high	1179:1186	arg1	cells					1188:1192	CD44high cells	1179:1192	CD44high cells	1179:1192	We observed that EVs released by CD44high cells induced the proliferation and activation of colon fibroblasts more strongly than CD44low cells.
35216292	0	34	dep	Different	75:83	arg1	Extracellular					85:97	Extracellular	85:97	Extracellular	85:97	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	3	35	theme	molecular	434:442	arg1	composition					444:454	the molecular composition	430:454	the molecular composition of the releasing cells	430:477	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	4	36	theme	tissue	766:771	arg1	heterogeneity					719:731	the cellular and molecular heterogeneity	692:731	the cellular and molecular heterogeneity of the original epithelial tumor tissue	692:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	5	37	theme	EV	936:937	arg1	release					939:945	an increased EV release	923:945	an increased EV release	923:945	Interestingly, we detected an increased EV release by CD44high CRC cells.
35216292	3	38	theme	EV	602:603	arg1	release					605:611	EV release	602:611	EV release	602:611	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	1	39	theme	Extracellular	125:137	arg1	EV					149:150	EV	149:150	EV	149:150	Extracellular vesicles (EV) are released by virtually all cells and they transport biologically important molecules from the release site to target cells.
35216292	1	39	theme	Extracellular	125:137	arg1	vesicles					139:146	Extracellular vesicles	125:146	Extracellular vesicles (EV)	125:151	Extracellular vesicles (EV) are released by virtually all cells and they transport biologically important molecules from the release site to target cells.
35216292	3	40	from	impact	545:550	arg1	intensity					589:597	the intensity	585:597	the intensity of EV release	585:611	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	5	41	theme	increased	926:934	arg1	release					939:945	an increased EV release	923:945	an increased EV release	923:945	Interestingly, we detected an increased EV release by CD44high CRC cells.
35216292	0	42	theme	Cancer	43:48	arg1	Subpopulations					55:68	Colorectal Cancer Cell Subpopulations	32:68	Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity	32:122	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	3	43	theme	cells	473:477	arg1	composition					444:454	the molecular composition	430:454	the molecular composition of the releasing cells	430:477	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	9	44	theme	cell-released	1493:1505	arg1	EVs					1507:1509	CRC cell-released EVs	1489:1509	CRC cell-released EVs	1489:1509	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	3	45	theme	release	605:611	arg1	intensity					589:597	the intensity	585:597	the intensity of EV release	585:611	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	9	46	dep	identified	1399:1408	arg1	proved					1477:1482	proved	1477:1482	identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation	1399:1584	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	6	47	theme	miRNA	1001:1005	arg1	cargos					1007:1012	the miRNA cargos	997:1012	the miRNA cargos of CD44high and CD44low cell derived EVs	997:1053	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	1	48	theme	important	221:229	arg1	molecules					231:239	biologically important molecules	208:239	biologically important molecules	208:239	Extracellular vesicles (EV) are released by virtually all cells and they transport biologically important molecules from the release site to target cells.
35216292	2	49	theme	major	376:380	arg1	issue					389:393	a major health issue	374:393	a major health issue	374:393	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	0	50	theme	Colorectal	32:41	arg1	Subpopulations					55:68	Colorectal Cancer Cell Subpopulations	32:68	Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity	32:122	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	9	51	theme	releasing	1447:1455	arg1	capabilities					1457:1468	different EV releasing capabilities	1434:1468	different EV releasing capabilities	1434:1468	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	8	52	theme	miRNA	1362:1366	arg1	cargo					1368:1372	the miRNA cargo	1358:1372	the miRNA cargo of EVs	1358:1379	However, this effect was due to the higher EV number rather than to the miRNA cargo of EVs.
35216292	2	53	theme	health	382:387	arg1	issue					389:393	a major health issue	374:393	a major health issue	374:393	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	9	54	theme	EV	1444:1445	arg1	capabilities					1457:1468	different EV releasing capabilities	1434:1468	different EV releasing capabilities	1434:1468	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	4	55	theme	CD44high	789:796	arg1	cells					798:802	CD44high cells	789:802	CD44high cells	789:802	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	6	56	theme	derived	1043:1049	arg1	EVs					1051:1053	CD44low cell derived EVs	1030:1053	CD44low cell derived EVs	1030:1053	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	2	57	theme	death	341:345	arg1	cases					347:351	cancer-related death cases	326:351	cancer-related death cases	326:351	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	0	58	theme	Cell	50:53	arg1	Subpopulations					55:68	Colorectal Cancer Cell Subpopulations	32:68	Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity	32:122	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	8	59	theme	EV	1333:1334	arg1	number					1336:1341	the higher EV number	1322:1341	the higher EV number	1322:1341	However, this effect was due to the higher EV number rather than to the miRNA cargo of EVs.
35216292	8	60	theme	higher	1326:1331	arg1	number					1336:1341	the higher EV number	1322:1341	the higher EV number	1322:1341	However, this effect was due to the higher EV number rather than to the miRNA cargo of EVs.
35216292	2	61	theme	cancer-related	326:339	arg1	cases					347:351	cancer-related death cases	326:351	cancer-related death cases	326:351	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	3	62	theme	tumor	522:526	arg1	diagnostics					528:538	tumor diagnostics	522:538	tumor diagnostics	522:538	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	9	63	theme	miRNA-independent	1518:1534	arg1	effect					1536:1541	a miRNA-independent effect	1516:1541	a miRNA-independent effect	1516:1541	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	8	64	theme	EVs	1377:1379	arg1	cargo					1368:1372	the miRNA cargo	1358:1372	the miRNA cargo of EVs	1358:1379	However, this effect was due to the higher EV number rather than to the miRNA cargo of EVs.
35216292	4	65	theme	tumor	760:764	arg1	tissue					766:771	the original epithelial tumor tissue	736:771	the original epithelial tumor tissue	736:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	9	66	theme	CRC	1410:1412	arg1	subpopulations					1414:1427	CRC subpopulations	1410:1427	CRC subpopulations with different EV releasing capabilities and we	1410:1475	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	4	67	theme	molecular	709:717	arg1	heterogeneity					719:731	the cellular and molecular heterogeneity	692:731	the cellular and molecular heterogeneity of the original epithelial tumor tissue	692:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	4	68	link	patient-derived	652:666	arg1	organoids					668:676	CRC patient-derived organoids	648:676	CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue	648:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	6	69	link	derived	1043:1049	arg1	EVs					1051:1053	CD44low cell derived EVs	1030:1053	CD44low cell derived EVs	1030:1053	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	4	70	theme	epithelial	749:758	arg1	tissue					766:771	the original epithelial tumor tissue	736:771	the original epithelial tumor tissue	736:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
35216292	0	71	with	Subpopulations	55:68	arg1	Capacity					115:122	Different Extracellular Vesicle Release Capacity	75:122	Different Extracellular Vesicle Release Capacity	75:122	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	6	72	theme	EVs	1051:1053	arg1	cargos					1007:1012	the miRNA cargos	997:1012	the miRNA cargos of CD44high and CD44low cell derived EVs	997:1053	In addition, we found that the miRNA cargos of CD44high and CD44low cell derived EVs largely overlapped and only four miRNAs were specific for one of the above subpopulations.
35216292	0	73	theme	Different	75:83	arg1	Capacity					115:122	Different Extracellular Vesicle Release Capacity	75:122	Different Extracellular Vesicle Release Capacity	75:122	CD44 Expression Intensity Marks Colorectal Cancer Cell Subpopulations with Different Extracellular Vesicle Release Capacity.
35216292	3	74	theme	intratumoral	555:566	arg1	heterogeneity					568:580	intratumoral heterogeneity	555:580	intratumoral heterogeneity	555:580	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	7	75	theme	colon	1238:1242	arg1	fibroblasts					1244:1254	colon fibroblasts	1238:1254	colon fibroblasts	1238:1254	We observed that EVs released by CD44high cells induced the proliferation and activation of colon fibroblasts more strongly than CD44low cells.
35216292	2	76	dep	cancer	291:296	arg1	cause					317:321	a leading cause	307:321	a leading cause of cancer-related death cases	307:351	Colorectal cancer (CRC) is a leading cause of cancer-related death cases, thus, it represents a major health issue.
35216292	1	77	theme	release	250:256	arg1	site					258:261	the release site	246:261	the release site to target cells	246:277	Extracellular vesicles (EV) are released by virtually all cells and they transport biologically important molecules from the release site to target cells.
35216292	3	78	theme	heterogeneity	568:580	arg1	impact					545:550	the impact	541:550	the impact of intratumoral heterogeneity on the intensity of EV release	541:611	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	3	78	theme	heterogeneity	568:580	arg1	unknown					630:636	unknown	630:636	unknown	630:636	Although the EV cargo may reflect the molecular composition of the releasing cells and thus, EVs may hold a great promise for tumor diagnostics, the impact of intratumoral heterogeneity on the intensity of EV release is still largely unknown.
35216292	9	79	theme	different	1434:1442	arg1	capabilities					1457:1468	different EV releasing capabilities	1434:1468	different EV releasing capabilities	1434:1468	Collectively, we identified CRC subpopulations with different EV releasing capabilities and we proved that CRC cell-released EVs have a miRNA-independent effect on fibroblast proliferation and activation.
35216292	4	80	theme	patient-derived	652:666	arg1	organoids					668:676	CRC patient-derived organoids	648:676	CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue	648:771	By using CRC patient-derived organoids that maintain the cellular and molecular heterogeneity of the original epithelial tumor tissue, we proved that CD44high cells produce more organoids with a higher proliferation intensity, as compared to CD44low cells.
36748483	6	0	theme	fatty	631:635	arg1	acids					637:641	The main fatty acids	622:641	The main fatty acids of the strain	622:655	The main fatty acids of the strain is the C18 : 1ω9c and C16 : 0.
36748483	6	0	theme	fatty	631:635	arg1	C18 					664:667	C18 	664:667	C18 	664:667	The main fatty acids of the strain is the C18 : 1ω9c and C16 : 0.
36748483	7	1	theme	G+C	692:694	arg1	%					736:736	62.8 mol %	727:736	62.8 mol %	727:736	The G+C content of the genomic DNA was 62.8 mol %.
36748483	7	1	theme	G+C	692:694	arg1	content					696:702	The G+C content	688:702	The G+C content of the genomic DNA	688:721	The G+C content of the genomic DNA was 62.8 mol %.
36748483	6	2	dep	C18 	664:667	arg1	the					660:662	the	660:662	the	660:662	The main fatty acids of the strain is the C18 : 1ω9c and C16 : 0.
36748483	2	3	attach	isolated	99:106	arg2	bacteria					90:97	A lactic acid bacteria	76:97	A lactic acid bacteria isolated from pig faeces	76:122	A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach.
36748483	2	3	attach	isolated	99:106	arg1	faeces					117:122	pig faeces	113:122	pig faeces	113:122	A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach.
36748483	6	4	theme	main	626:629	arg1	acids					637:641	The main fatty acids	622:641	The main fatty acids of the strain	622:655	The main fatty acids of the strain is the C18 : 1ω9c and C16 : 0.
36748483	6	4	theme	main	626:629	arg1	C18 					664:667	C18 	664:667	C18 	664:667	The main fatty acids of the strain is the C18 : 1ω9c and C16 : 0.
36748483	1	5	theme	pig	54:56	arg1	farm					58:61	pig farm	54:61	pig farm	54:61	nov., isolated from pig farm faeces dump.
36748483	4	6	theme	16S	280:282	arg1	rRNA					284:287	16S rRNA	280:287	16S rRNA gene sequence	280:301	Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus; however, the similarity to other homologues within the genus was <98 %.
36748483	4	7	theme	gene	289:292	arg1	sequence					294:301	16S rRNA gene sequence	280:301	16S rRNA gene sequence	280:301	Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus; however, the similarity to other homologues within the genus was <98 %.
36748483	1	8	attach	isolated	40:47	arg2	nov.					34:37	nov.	34:37	nov.	34:37	nov., isolated from pig farm faeces dump.
36748483	1	8	attach	isolated	40:47	arg1	farm					58:61	pig farm	54:61	pig farm	54:61	nov., isolated from pig farm faeces dump.
36748483	4	9	theme	other	399:403	arg1	homologues					405:414	other homologues	399:414	other homologues	399:414	Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus; however, the similarity to other homologues within the genus was <98 %.
36748483	4	10	theme	rRNA	284:287	arg1	sequence					294:301	16S rRNA gene sequence	280:301	16S rRNA gene sequence	280:301	Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus; however, the similarity to other homologues within the genus was <98 %.
36748483	8	11	theme	polar	749:753	arg1	diphosphatidylglycerol					767:788	diphosphatidylglycerol	767:788	diphosphatidylglycerol	767:788	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36748483	8	11	theme	polar	749:753	arg1	lipids					755:760	The major polar lipids	739:760	The major polar lipids	739:760	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36748483	8	12	theme	major	743:747	arg1	diphosphatidylglycerol					767:788	diphosphatidylglycerol	767:788	diphosphatidylglycerol	767:788	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36748483	8	12	theme	major	743:747	arg1	lipids					755:760	The major polar lipids	739:760	The major polar lipids	739:760	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36748483	5	13	theme	adjacent	546:553	arg1	sub-cluster					534:544	a sub-cluster	532:544	a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis	532:619	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	0	14	theme	Lacticaseibacillus	0:17	arg1	sp					30:31	Lacticaseibacillus kribbianus sp	0:31	Lacticaseibacillus kribbianus sp.	0:32	Lacticaseibacillus kribbianus sp.
36748483	0	15	theme	kribbianus	19:28	arg1	sp					30:31	Lacticaseibacillus kribbianus sp	0:31	Lacticaseibacillus kribbianus sp.	0:32	Lacticaseibacillus kribbianus sp.
36748483	3	16	theme	strain	184:189	arg1	Cells					171:175	Cells	171:175	Cells of the strain	171:189	Cells of the strain were Gram-stain-positive, rod-shaped and facultative anaerobic.
36748483	4	17	theme	sequence	294:301	arg1	analysis					268:275	Phylogenetic analysis	255:275	Phylogenetic analysis of 16S rRNA gene sequence	255:301	Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus; however, the similarity to other homologues within the genus was <98 %.
36748483	3	18	dep	Gram-stain-positive	196:214	arg1	anaerobic					244:252	anaerobic	244:252	anaerobic	244:252	Cells of the strain were Gram-stain-positive, rod-shaped and facultative anaerobic.
36748483	10	19	theme	=KCTC	938:942	arg1	YH-lac21T					927:935	YH-lac21T	927:935	YH-lac21T (=KCTC 21185=JCM 34953)	927:959	Thus, YH-lac21T (=KCTC 21185=JCM 34953) represents a novel species.
36748483	10	19	theme	=KCTC	938:942	arg1	21185=JCM					944:952	=KCTC 21185=JCM 34953	938:958	=KCTC 21185=JCM 34953	938:958	Thus, YH-lac21T (=KCTC 21185=JCM 34953) represents a novel species.
36748483	5	20	theme	housekeeping	456:467	arg1	pheS					485:488	pheS	485:488	pheS	485:488	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	5	20	theme	housekeeping	456:467	arg1	sequences					474:482	housekeeping gene sequences	456:482	housekeeping gene sequences (pheS and recA)	456:498	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	5	20	theme	housekeeping	456:467	arg1	recA					494:497	recA	494:497	recA	494:497	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	11	21	theme	Lacticaseibacillus	998:1015	arg1	sp					1028:1029	The name Lacticaseibacillus kribbianus sp	989:1029	The name Lacticaseibacillus kribbianus sp.	989:1030	The name Lacticaseibacillus kribbianus sp.
36748483	5	22	theme	gene	469:472	arg1	pheS					485:488	pheS	485:488	pheS	485:488	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	5	22	theme	gene	469:472	arg1	sequences					474:482	housekeeping gene sequences	456:482	housekeeping gene sequences (pheS and recA)	456:498	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	5	22	theme	gene	469:472	arg1	recA					494:497	recA	494:497	recA	494:497	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	9	23	theme	cell-wall	855:863	arg1	peptidoglycan					865:877	The cell-wall peptidoglycan	851:877	The cell-wall peptidoglycan	851:877	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
36748483	5	24	theme	sequences	474:482	arg1	Analysis					444:451	Analysis	444:451	Analysis of housekeeping gene sequences (pheS and recA)	444:498	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	2	25	theme	acid	85:88	arg1	bacteria					90:97	A lactic acid bacteria	76:97	A lactic acid bacteria isolated from pig faeces	76:122	A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach.
36748483	2	26	theme	polyphasic	150:159	arg1	approach					161:168	a polyphasic approach	148:168	a polyphasic approach	148:168	A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach.
36748483	2	27	theme	lactic	78:83	arg1	bacteria					90:97	A lactic acid bacteria	76:97	A lactic acid bacteria isolated from pig faeces	76:122	A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach.
36748483	11	28	theme	kribbianus	1017:1026	arg1	sp					1028:1029	The name Lacticaseibacillus kribbianus sp	989:1029	The name Lacticaseibacillus kribbianus sp.	989:1030	The name Lacticaseibacillus kribbianus sp.
36748483	4	29	theme	genus	346:350	arg1	Lacticaseibacillus					352:369	the genus Lacticaseibacillus	342:369	the genus Lacticaseibacillus	342:369	Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus; however, the similarity to other homologues within the genus was <98 %.
36748483	11	30	theme	name	993:996	arg1	sp					1028:1029	The name Lacticaseibacillus kribbianus sp	989:1029	The name Lacticaseibacillus kribbianus sp.	989:1030	The name Lacticaseibacillus kribbianus sp.
36748483	6	31	theme	strain	650:655	arg1	acids					637:641	The main fatty acids	622:641	The main fatty acids of the strain	622:655	The main fatty acids of the strain is the C18 : 1ω9c and C16 : 0.
36748483	6	31	theme	strain	650:655	arg1	C18 					664:667	C18 	664:667	C18 	664:667	The main fatty acids of the strain is the C18 : 1ω9c and C16 : 0.
36748483	7	32	theme	genomic	711:717	arg1	DNA					719:721	the genomic DNA	707:721	the genomic DNA	707:721	The G+C content of the genomic DNA was 62.8 mol %.
36748483	9	33	theme	meso-diaminopimelic	895:913	arg1	acid					915:918	meso-diaminopimelic acid	895:918	meso-diaminopimelic acid	895:918	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
36748483	4	34	theme	Phylogenetic	255:266	arg1	analysis					268:275	Phylogenetic analysis	255:275	Phylogenetic analysis of 16S rRNA gene sequence	255:301	Phylogenetic analysis of 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus; however, the similarity to other homologues within the genus was <98 %.
36748483	7	35	theme	DNA	719:721	arg1	%					736:736	62.8 mol %	727:736	62.8 mol %	727:736	The G+C content of the genomic DNA was 62.8 mol %.
36748483	7	35	theme	DNA	719:721	arg1	content					696:702	The G+C content	688:702	The G+C content of the genomic DNA	688:721	The G+C content of the genomic DNA was 62.8 mol %.
36748483	5	36	dep	sequences	474:482	arg1	pheS					485:488	pheS	485:488	pheS	485:488	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	5	36	dep	sequences	474:482	arg1	sequences					474:482	housekeeping gene sequences	456:482	housekeeping gene sequences (pheS and recA)	456:498	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	5	36	dep	sequences	474:482	arg1	recA					494:497	recA	494:497	recA	494:497	Analysis of housekeeping gene sequences (pheS and recA) revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus absianus and Lacticaseibacillus daqingensis.
36748483	2	37	theme	pig	113:115	arg1	faeces					117:122	pig faeces	113:122	pig faeces	113:122	A lactic acid bacteria isolated from pig faeces was characterized using a polyphasic approach.
36748483	10	38	theme	novel	974:978	arg1	species					980:986	a novel species	972:986	a novel species	972:986	Thus, YH-lac21T (=KCTC 21185=JCM 34953) represents a novel species.
36748483	9	39	contain	contain	887:893	arg2	acid					915:918	meso-diaminopimelic acid	895:918	meso-diaminopimelic acid	895:918	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
36748483	9	39	contain	contain	887:893	arg1	peptidoglycan					865:877	The cell-wall peptidoglycan	851:877	The cell-wall peptidoglycan	851:877	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
35337221	4	0	theme	OAE	927:929	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	0	theme	OAE	927:929	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	1	theme	equivalent	915:924	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	1	theme	equivalent	915:924	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	2	theme	acid	829:832	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	2	theme	acid	829:832	arg1	/g					887:888	38.2 mg quercetin equivalent (QE)/g	854:888	38.2 mg quercetin equivalent (QE)/g	854:888	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	2	theme	acid	829:832	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	2	theme	acid	829:832	arg1	content					800:806	the total content	790:806	the total content	790:806	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	3	theme	Chemical	649:656	arg1	analysis					670:677	Chemical composition analysis	649:677	Chemical composition analysis	649:677	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	4	theme	acid	910:913	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	4	theme	acid	910:913	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	5	theme	gallic	822:827	arg1	acid					829:832	30.6 mg gallic acid	814:832	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	1	6	theme	Semiliquidambar	153:167	arg1	WESCL					187:191	WESCL	187:191	WESCL	187:191	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	1	6	theme	Semiliquidambar	153:167	arg1	leaf					181:184	Semiliquidambar cathayensis leaf	153:184	Semiliquidambar cathayensis leaf (WESCL)	153:192	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	4	7	theme	flavonoids	746:755	arg1	flavonoids					746:755	flavonoids	746:755	flavonoids	746:755	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	7	theme	flavonoids	746:755	arg1	amounts					715:721	high amounts	710:721	high amounts of phenolic compounds, flavonoids, and triterpenoid saponins	710:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	7	theme	flavonoids	746:755	arg1	compounds					735:743	phenolic compounds	726:743	phenolic compounds	726:743	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	7	theme	flavonoids	746:755	arg1	saponins					775:782	triterpenoid saponins	762:782	triterpenoid saponins	762:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	2	8	from	production	325:334	arg1	macrophages					420:430	LPS-stimulated RAW 264.7 macrophages	395:430	LPS-stimulated RAW 264.7 macrophages	395:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	4	9	theme	phenolic	726:733	arg1	compounds					735:743	phenolic compounds	726:743	phenolic compounds	726:743	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	10	theme	high	710:713	arg1	flavonoids					746:755	flavonoids	746:755	flavonoids	746:755	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	10	theme	high	710:713	arg1	amounts					715:721	high amounts	710:721	high amounts of phenolic compounds, flavonoids, and triterpenoid saponins	710:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	10	theme	high	710:713	arg1	compounds					735:743	phenolic compounds	726:743	phenolic compounds	726:743	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	10	theme	high	710:713	arg1	saponins					775:782	triterpenoid saponins	762:782	triterpenoid saponins	762:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	11	theme	QE	884:885	arg1	/g					887:888	38.2 mg quercetin equivalent (QE)/g	854:888	38.2 mg quercetin equivalent (QE)/g	854:888	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	11	theme	QE	884:885	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	5	12	theme	anti-inflammatory	1022:1038	arg1	agent					1040:1044	a naturally-occurring anti-inflammatory agent	1000:1044	a naturally-occurring anti-inflammatory agent	1000:1044	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	5	12	theme	anti-inflammatory	1022:1038	arg1	WESCL					991:995	WESCL	991:995	WESCL	991:995	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	4	13	theme	equivalent	834:843	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	13	theme	equivalent	834:843	arg1	/g					887:888	38.2 mg quercetin equivalent (QE)/g	854:888	38.2 mg quercetin equivalent (QE)/g	854:888	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	13	theme	equivalent	834:843	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	13	theme	equivalent	834:843	arg1	content					800:806	the total content	790:806	the total content	790:806	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	3	14	from	level	610:614	arg1	mice					643:646	mice	643:646	mice	643:646	Furthermore, an in vivo study revealed that WESCL could alleviate the disease development of osteoarthritis (OA) and decrease the level of interleukin-6 (IL-6) in mice.
35337221	2	15	theme	oxide	346:350	arg1	metamorphosis					306:318	the cell metamorphosis	297:318	the cell metamorphosis	297:318	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	15	theme	oxide	346:350	arg1	production					325:334	the production	321:334	the production of nitric oxide (NO)	321:355	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	15	theme	oxide	346:350	arg1	species					378:384	reactive oxygen species	362:384	reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages	362:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	15	theme	oxide	346:350	arg1	ROS					387:389	ROS	387:389	ROS	387:389	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	3	16	theme	in vivo	496:502	arg1	study					504:508	an in vivo study	493:508	an in vivo study	493:508	Furthermore, an in vivo study revealed that WESCL could alleviate the disease development of osteoarthritis (OA) and decrease the level of interleukin-6 (IL-6) in mice.
35337221	1	17	theme	cathayensis	169:179	arg1	WESCL					187:191	WESCL	187:191	WESCL	187:191	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	1	17	theme	cathayensis	169:179	arg1	leaf					181:184	Semiliquidambar cathayensis leaf	153:184	Semiliquidambar cathayensis leaf (WESCL)	153:192	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	0	18	theme	extract	40:46	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.	0:83	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.
35337221	4	19	theme	compounds	735:743	arg1	flavonoids					746:755	flavonoids	746:755	flavonoids	746:755	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	19	theme	compounds	735:743	arg1	amounts					715:721	high amounts	710:721	high amounts of phenolic compounds, flavonoids, and triterpenoid saponins	710:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	19	theme	compounds	735:743	arg1	compounds					735:743	phenolic compounds	726:743	phenolic compounds	726:743	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	19	theme	compounds	735:743	arg1	saponins					775:782	triterpenoid saponins	762:782	triterpenoid saponins	762:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	1	20	theme	leaf	181:184	arg1	extract					142:148	the water extract	132:148	the water extract of Semiliquidambar cathayensis leaf (WESCL)	132:192	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	4	21	theme	30.6 mg	814:820	arg1	acid					829:832	30.6 mg gallic acid	814:832	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	22	theme	oleanolic	900:908	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	22	theme	oleanolic	900:908	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	2	23	theme	reactive	362:369	arg1	ROS					387:389	ROS	387:389	ROS	387:389	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	23	theme	reactive	362:369	arg1	species					378:384	reactive oxygen species	362:384	reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages	362:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	0	24	theme	Anti-inflammatory	0:16	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.	0:83	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.
35337221	5	25	theme	diseases	1123:1130	arg1	treatment					1089:1097	the treatment	1085:1097	the treatment of inflammation-related diseases	1085:1130	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	5	26	theme	naturally-occurring	1002:1020	arg1	agent					1040:1044	a naturally-occurring anti-inflammatory agent	1000:1044	a naturally-occurring anti-inflammatory agent	1000:1044	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	5	26	theme	naturally-occurring	1002:1020	arg1	WESCL					991:995	WESCL	991:995	WESCL	991:995	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	2	27	theme	RAW	410:412	arg1	macrophages					420:430	LPS-stimulated RAW 264.7 macrophages	395:430	LPS-stimulated RAW 264.7 macrophages	395:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	4	28	theme	quercetin	862:870	arg1	/g					887:888	38.2 mg quercetin equivalent (QE)/g	854:888	38.2 mg quercetin equivalent (QE)/g	854:888	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	28	theme	quercetin	862:870	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	2	29	theme	LPS-stimulated	395:408	arg1	macrophages					420:430	LPS-stimulated RAW 264.7 macrophages	395:430	LPS-stimulated RAW 264.7 macrophages	395:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	5	30	theme	potent	1062:1067	arg1	application					1069:1079	its potent application	1058:1079	its potent application for the treatment of inflammation-related diseases	1058:1130	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	4	31	theme	38.2 mg	854:860	arg1	/g					887:888	38.2 mg quercetin equivalent (QE)/g	854:888	38.2 mg quercetin equivalent (QE)/g	854:888	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	31	theme	38.2 mg	854:860	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	2	32	theme	cell	301:304	arg1	metamorphosis					306:318	the cell metamorphosis	297:318	the cell metamorphosis	297:318	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	33	theme	cytotoxic	449:457	arg1	effect					459:464	no cytotoxic effect	446:464	no cytotoxic effect	446:464	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	4	34	contain	contained	700:708	arg2	flavonoids					746:755	flavonoids	746:755	flavonoids	746:755	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	34	contain	contained	700:708	arg1	WESCL					694:698	WESCL	694:698	WESCL	694:698	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	34	contain	contained	700:708	arg2	compounds					735:743	phenolic compounds	726:743	phenolic compounds	726:743	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	34	contain	contained	700:708	arg2	amounts					715:721	high amounts	710:721	high amounts of phenolic compounds, flavonoids, and triterpenoid saponins	710:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	34	contain	contained	700:708	arg2	saponins					775:782	triterpenoid saponins	762:782	triterpenoid saponins	762:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	0	35	theme	water	34:38	arg1	extract					40:46	the water extract	30:46	the water extract of Semiliquidambar cathayensis leaf	30:82	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.
35337221	4	36	theme	equivalent	872:881	arg1	/g					887:888	38.2 mg quercetin equivalent (QE)/g	854:888	38.2 mg quercetin equivalent (QE)/g	854:888	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	36	theme	equivalent	872:881	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	3	37	theme	osteoarthritis	573:586	arg1	development					558:568	the disease development	546:568	the disease development of osteoarthritis (OA)	546:591	Furthermore, an in vivo study revealed that WESCL could alleviate the disease development of osteoarthritis (OA) and decrease the level of interleukin-6 (IL-6) in mice.
35337221	0	38	theme	Semiliquidambar	51:65	arg1	leaf					79:82	Semiliquidambar cathayensis leaf	51:82	Semiliquidambar cathayensis leaf	51:82	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.
35337221	4	39	theme	100.5 mg	891:898	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	39	theme	100.5 mg	891:898	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	3	40	theme	disease	550:556	arg1	development					558:568	the disease development	546:568	the disease development of osteoarthritis (OA)	546:591	Furthermore, an in vivo study revealed that WESCL could alleviate the disease development of osteoarthritis (OA) and decrease the level of interleukin-6 (IL-6) in mice.
35337221	1	41	theme	anti-inflammatory	104:120	arg1	effect					122:127	the anti-inflammatory effect	100:127	the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL)	100:192	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	4	42	theme	GAE	846:848	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	42	theme	GAE	846:848	arg1	/g					887:888	38.2 mg quercetin equivalent (QE)/g	854:888	38.2 mg quercetin equivalent (QE)/g	854:888	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	42	theme	GAE	846:848	arg1	/g					931:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	100.5 mg oleanolic acid equivalent (OAE)/g	891:932	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	42	theme	GAE	846:848	arg1	content					800:806	the total content	790:806	the total content	790:806	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	5	43	theme	present	953:959	arg1	study					961:965	The present study	949:965	The present study	949:965	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	4	44	theme	total	794:798	arg1	/g					850:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	30.6 mg gallic acid equivalent (GAE)/g	814:851	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	44	theme	total	794:798	arg1	content					800:806	the total content	790:806	the total content	790:806	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	2	45	from	species	378:384	arg1	macrophages					420:430	LPS-stimulated RAW 264.7 macrophages	395:430	LPS-stimulated RAW 264.7 macrophages	395:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	46	theme	nitric	339:344	arg1	NO					353:354	NO	353:354	NO	353:354	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	46	theme	nitric	339:344	arg1	oxide					346:350	nitric oxide	339:350	nitric oxide (NO)	339:355	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	5	47	theme	inflammation-related	1102:1121	arg1	diseases					1123:1130	inflammation-related diseases	1102:1130	inflammation-related diseases	1102:1130	The present study successfully identified WESCL as a naturally-occurring anti-inflammatory agent, supporting its potent application for the treatment of inflammation-related diseases.
35337221	4	48	theme	saponins	775:782	arg1	flavonoids					746:755	flavonoids	746:755	flavonoids	746:755	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	48	theme	saponins	775:782	arg1	amounts					715:721	high amounts	710:721	high amounts of phenolic compounds, flavonoids, and triterpenoid saponins	710:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	48	theme	saponins	775:782	arg1	compounds					735:743	phenolic compounds	726:743	phenolic compounds	726:743	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	4	48	theme	saponins	775:782	arg1	saponins					775:782	triterpenoid saponins	762:782	triterpenoid saponins	762:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	3	49	theme	interleukin-6	619:631	arg1	level					610:614	the level	606:614	the level of interleukin-6 (IL-6) in mice	606:646	Furthermore, an in vivo study revealed that WESCL could alleviate the disease development of osteoarthritis (OA) and decrease the level of interleukin-6 (IL-6) in mice.
35337221	2	50	theme	anti-inflammatory	244:260	arg1	activity					262:269	anti-inflammatory activity	244:269	anti-inflammatory activity	244:269	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	1	51	theme	first	216:220	arg1	time					222:225	the first time	212:225	the first time	212:225	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	0	52	theme	leaf	79:82	arg1	extract					40:46	the water extract	30:46	the water extract of Semiliquidambar cathayensis leaf	30:82	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.
35337221	4	53	theme	triterpenoid	762:773	arg1	saponins					775:782	triterpenoid saponins	762:782	triterpenoid saponins	762:782	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35337221	1	54	theme	water	136:140	arg1	extract					142:148	the water extract	132:148	the water extract of Semiliquidambar cathayensis leaf (WESCL)	132:192	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	2	55	theme	oxygen	371:376	arg1	ROS					387:389	ROS	387:389	ROS	387:389	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	2	55	theme	oxygen	371:376	arg1	species					378:384	reactive oxygen species	362:384	reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages	362:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	0	56	theme	cathayensis	67:77	arg1	leaf					79:82	Semiliquidambar cathayensis leaf	51:82	Semiliquidambar cathayensis leaf	51:82	Anti-inflammatory activity of the water extract of Semiliquidambar cathayensis leaf.
35337221	1	57	theme	extract	142:148	arg1	effect					122:127	the anti-inflammatory effect	100:127	the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL)	100:192	In this study, the anti-inflammatory effect of the water extract of Semiliquidambar cathayensis leaf (WESCL) was evaluated for the first time.
35337221	2	58	from	metamorphosis	306:318	arg1	macrophages					420:430	LPS-stimulated RAW 264.7 macrophages	395:430	LPS-stimulated RAW 264.7 macrophages	395:430	WESCL exhibited anti-inflammatory activity by significantly reducing the cell metamorphosis, the production of nitric oxide (NO), and reactive oxygen species (ROS) in LPS-stimulated RAW 264.7 macrophages while showing no cytotoxic effect to the cells.
35337221	4	59	theme	composition	658:668	arg1	analysis					670:677	Chemical composition analysis	649:677	Chemical composition analysis	649:677	Chemical composition analysis indicated that WESCL contained high amounts of phenolic compounds, flavonoids, and triterpenoid saponins, with the total content being 30.6 mg gallic acid equivalent (GAE)/g, 38.2 mg quercetin equivalent (QE)/g, 100.5 mg oleanolic acid equivalent (OAE)/g, respectively.
35041881	7	0	theme	@	1164:1164	arg1	fluorescence					1171:1182	MCM@CS@Au fluorescence	1161:1182	MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor	1161:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	7	1	theme	base	1079:1082	arg1	pairs					1084:1088	the base pairs	1075:1088	the base pairs in the hairpin	1075:1103	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	1	2	theme	CS	223:224	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	2	theme	CS	223:224	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	11	3	theme	non-invasive	1880:1891	arg1	imaging					1906:1912	non-invasive fluorescence imaging	1880:1912	non-invasive fluorescence imaging	1880:1912	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	7	4	theme	energy	1051:1056	arg1	quencher					1148:1155	a quencher	1146:1155	a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor	1146:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	7	4	theme	energy	1051:1056	arg1	transfer					1058:1065	the energy transfer	1047:1065	the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer	1047:1136	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	10	5	theme	toxicity	1590:1597	arg1	results					1599:1605	the toxicity results	1586:1605	the toxicity results	1586:1605	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	5	6	theme	release	805:811	arg1	mechanism					783:791	The mechanism	779:791	The mechanism of curcumin release	779:811	The mechanism of curcumin release was likewise investigated through different kinetic models.
35041881	3	7	theme	successful	524:533	arg1	approved					551:558	approved	551:558	approved	551:558	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	3	7	theme	successful	524:533	arg1	fabrication					535:545	The nanosystem successful fabrication	509:545	The nanosystem successful fabrication	509:545	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	1	8	theme	silica	197:202	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	8	theme	silica	197:202	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	6	9	theme	designed	906:913	arg1	nanosystem					915:924	the designed nanosystem	902:924	the designed nanosystem	902:924	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	2	10	theme	@	499:499	arg1	Au-Apt					500:505	MCM@CS@Au-Apt	493:505	MCM@CS@Au-Apt	493:505	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	2	10	theme	@	499:499	arg1	nanosystem					481:490	the nanosystem	477:490	the nanosystem (MCM@CS@Au-Apt)	477:506	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	10	11	theme	MUC-1-positive	1653:1666	arg1	cells					1674:1678	the MUC-1-positive tumor cells	1649:1678	the MUC-1-positive tumor cells	1649:1678	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	1	12	theme	chitosan	204:211	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	12	theme	chitosan	204:211	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	8	13	theme	desired	1360:1366	arg1	cells					1368:1372	the desired cells	1356:1372	the desired cells	1356:1372	On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells.
35041881	11	14	theme	nanotheranostic	1814:1828	arg1	system					1830:1835	a smart anticancer nanotheranostic system	1795:1835	a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery	1795:1944	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	11	14	theme	nanotheranostic	1814:1828	arg1	nanosystem					1761:1770	this nanosystem	1756:1770	this nanosystem	1756:1770	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	10	15	theme	MUC-1-negative	1685:1698	arg1	cells					1700:1704	MUC-1-negative cells	1685:1704	MUC-1-negative cells	1685:1704	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	6	16	theme	flow	1013:1016	arg1	cytometry					1018:1026	flow cytometry	1013:1026	flow cytometry	1013:1026	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	7	17	theme	MCM	1161:1163	arg1	fluorescence					1171:1182	MCM@CS@Au fluorescence	1161:1182	MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor	1161:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	1	18	theme	positive	278:285	arg1	cells					293:297	the MUC-1 positive tumor cells	268:297	the MUC-1 positive tumor cells	268:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	0	19	theme	@	17:17	arg1	gold					27:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	1	20	theme	cancer	132:137	arg1	system					155:160	A cancer nanotheranostic system	130:160	A cancer nanotheranostic system	130:160	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	5	21	theme	kinetic	857:863	arg1	models					865:870	different kinetic models	847:870	different kinetic models	847:870	The mechanism of curcumin release was likewise investigated through different kinetic models.
35041881	2	22	theme	@	496:496	arg1	Au-Apt					500:505	MCM@CS@Au-Apt	493:505	MCM@CS@Au-Apt	493:505	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	2	22	theme	@	496:496	arg1	nanosystem					481:490	the nanosystem	477:490	the nanosystem (MCM@CS@Au-Apt)	477:506	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	2	23	theme	anticancer	346:355	arg1	drug					357:360	an efficient herbal anticancer drug	326:360	an efficient herbal anticancer drug	326:360	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	0	24	theme	pH-sensitive	80:91	arg1	"					56:56	"on/off" optical biosensor and pH-sensitive theranostic platform	49:112	"on/off" optical biosensor and pH-sensitive theranostic platform against cancer	49:127	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	24	theme	pH-sensitive	80:91	arg1	platform					105:112	pH-sensitive theranostic platform	80:112	pH-sensitive theranostic platform	80:112	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	9	25	theme	MUC-1	1426:1430	arg1	strands					1453:1459	its double strands	1442:1459	its double strands separate under the low pH condition	1442:1495	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	9	25	theme	MUC-1	1426:1430	arg1	receptor					1432:1439	the MUC-1 receptor	1422:1439	the MUC-1 receptor	1422:1439	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	2	26	theme	efficient	329:337	arg1	drug					357:360	an efficient herbal anticancer drug	326:360	an efficient herbal anticancer drug	326:360	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	0	27	theme	Mesoporous	0:9	arg1	gold					27:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	28	dep	"	56:56	arg1	"					56:56	"on/off" optical biosensor and pH-sensitive theranostic platform	49:112	"on/off" optical biosensor and pH-sensitive theranostic platform against cancer	49:127	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	28	dep	"	56:56	arg1	platform					105:112	pH-sensitive theranostic platform	80:112	pH-sensitive theranostic platform	80:112	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	28	dep	"	56:56	arg1	biosensor					66:74	optical biosensor	58:74	optical biosensor	58:74	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	2	29	theme	chitosan-triphosphate	390:410	arg1	nanoparticles					412:424	chitosan-triphosphate nanoparticles	390:424	chitosan-triphosphate nanoparticles	390:424	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	8	30	theme	chitosan	1278:1285	arg1	nanoparticles					1287:1299	pH-sensitive chitosan nanoparticles	1265:1299	pH-sensitive chitosan nanoparticles	1265:1299	On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells.
35041881	7	31	theme	double	1108:1113	arg1	strands					1115:1121	double strands	1108:1121	double strands of DNA aptamer	1108:1136	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	9	32	theme	low	1480:1482	arg1	condition					1487:1495	the low pH condition	1476:1495	the low pH condition	1476:1495	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	7	33	theme	optical	1211:1217	arg1	biosensor					1219:1227	an "on/off" optical biosensor	1199:1227	an "on/off" optical biosensor	1199:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	9	34	from	state	1569:1573	arg1	recovery					1534:1541	the recovery	1530:1541	the recovery of the fluorescence ("On" state)	1530:1574	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	9	34	from	state	1569:1573	arg1	release					1518:1524	the drug release	1509:1524	the drug release	1509:1524	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	4	35	theme	molecular	747:755	arg1	simulations					766:776	full atomistic molecular dynamics simulations	732:776	full atomistic molecular dynamics simulations	732:776	Besides, the interaction between aptamer and curcumin was evaluated using full atomistic molecular dynamics simulations.
35041881	0	36	theme	@	26:26	arg1	gold					27:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	7	37	theme	on/off	1203:1208	arg1	biosensor					1219:1227	an "on/off" optical biosensor	1199:1227	an "on/off" optical biosensor	1199:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	6	38	from	potential	889:897	arg1	imaging					938:944	targeted imaging	929:944	targeted imaging	929:944	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	6	38	from	potential	889:897	arg1	delivery					956:963	drug delivery	951:963	drug delivery	951:963	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	3	39	theme	synthesis	568:576	arg1	step					578:581	each synthesis step	563:581	each synthesis step	563:581	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	4	40	theme	atomistic	737:745	arg1	simulations					766:776	full atomistic molecular dynamics simulations	732:776	full atomistic molecular dynamics simulations	732:776	Besides, the interaction between aptamer and curcumin was evaluated using full atomistic molecular dynamics simulations.
35041881	1	41	theme	@	212:212	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	41	theme	@	212:212	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	6	42	theme	fluorescence	985:996	arg1	microscopy					998:1007	fluorescence microscopy	985:1007	fluorescence microscopy	985:1007	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	4	43	theme	full	732:735	arg1	simulations					766:776	full atomistic molecular dynamics simulations	732:776	full atomistic molecular dynamics simulations	732:776	Besides, the interaction between aptamer and curcumin was evaluated using full atomistic molecular dynamics simulations.
35041881	7	44	theme	DNA	1126:1128	arg1	aptamer					1130:1136	DNA aptamer	1126:1136	DNA aptamer	1126:1136	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	7	45	theme	Au	1168:1169	arg1	fluorescence					1171:1182	MCM@CS@Au fluorescence	1161:1182	MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor	1161:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	1	46	theme	@	222:222	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	46	theme	@	222:222	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	7	47	theme	CS	1165:1166	arg1	fluorescence					1171:1182	MCM@CS@Au fluorescence	1161:1182	MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor	1161:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	1	48	theme	@	225:225	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	48	theme	@	225:225	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	5	49	theme	different	847:855	arg1	models					865:870	different kinetic models	847:870	different kinetic models	847:870	The mechanism of curcumin release was likewise investigated through different kinetic models.
35041881	5	50	theme	curcumin	796:803	arg1	release					805:811	curcumin release	796:811	curcumin release	796:811	The mechanism of curcumin release was likewise investigated through different kinetic models.
35041881	11	51	theme	particular	1849:1858	arg1	MUC-1					1872:1876	MUC-1	1872:1876	MUC-1	1872:1876	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	11	51	theme	particular	1849:1858	arg1	biomarkers					1860:1869	particular biomarkers	1849:1869	particular biomarkers (MUC-1)	1849:1877	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	9	52	theme	fluorescence	1550:1561	arg1	recovery					1534:1541	the recovery	1530:1541	the recovery of the fluorescence ("On" state)	1530:1574	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	9	52	theme	fluorescence	1550:1561	arg1	release					1518:1524	the drug release	1509:1524	the drug release	1509:1524	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	3	53	theme	fluorescence	631:642	arg1	spectroscopy					644:655	fluorescence spectroscopy	631:655	fluorescence spectroscopy	631:655	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	3	54	theme	nanosystem	513:522	arg1	approved					551:558	approved	551:558	approved	551:558	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	3	54	theme	nanosystem	513:522	arg1	fabrication					535:545	The nanosystem successful fabrication	509:545	The nanosystem successful fabrication	509:545	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	6	55	theme	targeted	929:936	arg1	imaging					938:944	targeted imaging	929:944	targeted imaging	929:944	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	4	56	theme	dynamics	757:764	arg1	simulations					766:776	full atomistic molecular dynamics simulations	732:776	full atomistic molecular dynamics simulations	732:776	Besides, the interaction between aptamer and curcumin was evaluated using full atomistic molecular dynamics simulations.
35041881	11	57	theme	fluorescence	1893:1904	arg1	imaging					1906:1912	non-invasive fluorescence imaging	1880:1912	non-invasive fluorescence imaging	1880:1912	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	1	58	theme	mesoporous	186:195	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	58	theme	mesoporous	186:195	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	10	59	theme	more	1628:1631	arg1	toxicity					1633:1640	more toxicity	1628:1640	more toxicity toward the MUC-1-positive tumor cells	1628:1678	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	6	60	theme	nanosystem	915:924	arg1	potential					889:897	the potential	885:897	the potential of the designed nanosystem in targeted imaging, and drug delivery	885:963	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	9	61	theme	"	1567:1567	arg1	state					1569:1573	" state	1567:1573	" state	1567:1573	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	10	62	theme	tumor	1668:1672	arg1	cells					1674:1678	the MUC-1-positive tumor cells	1649:1678	the MUC-1-positive tumor cells	1649:1678	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	1	63	theme	@	203:203	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	63	theme	@	203:203	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	9	64	from	recovery	1534:1541	arg1	state					1569:1573	" state	1567:1573	" state	1567:1573	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	2	65	theme	CS	497:498	arg1	Au-Apt					500:505	MCM@CS@Au-Apt	493:505	MCM@CS@Au-Apt	493:505	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	2	65	theme	CS	497:498	arg1	nanosystem					481:490	the nanosystem	477:490	the nanosystem (MCM@CS@Au-Apt)	477:506	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	11	66	theme	anticancer	1803:1812	arg1	system					1830:1835	a smart anticancer nanotheranostic system	1795:1835	a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery	1795:1944	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	11	66	theme	anticancer	1803:1812	arg1	nanosystem					1761:1770	this nanosystem	1756:1770	this nanosystem	1756:1770	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	0	67	theme	on/off	50:55	arg1	"					56:56	"on/off" optical biosensor and pH-sensitive theranostic platform	49:112	"on/off" optical biosensor and pH-sensitive theranostic platform against cancer	49:127	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	67	theme	on/off	50:55	arg1	platform					105:112	pH-sensitive theranostic platform	80:112	pH-sensitive theranostic platform	80:112	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	67	theme	on/off	50:55	arg1	biosensor					66:74	optical biosensor	58:74	optical biosensor	58:74	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	7	68	from	pairs	1084:1088	arg1	hairpin					1097:1103	the hairpin	1093:1103	the hairpin	1093:1103	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	10	69	contain	had	1624:1626	arg2	toxicity					1633:1640	more toxicity	1628:1640	more toxicity toward the MUC-1-positive tumor cells	1628:1678	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	10	69	contain	had	1624:1626	arg1	nanosystem					1613:1622	this nanosystem	1608:1622	this nanosystem	1608:1622	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	0	70	theme	optical	58:64	arg1	"					56:56	"on/off" optical biosensor and pH-sensitive theranostic platform	49:112	"on/off" optical biosensor and pH-sensitive theranostic platform against cancer	49:127	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	70	theme	optical	58:64	arg1	biosensor					66:74	optical biosensor	58:74	optical biosensor	58:74	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	1	71	theme	tumor	287:291	arg1	cells					293:297	the MUC-1 positive tumor cells	268:297	the MUC-1 positive tumor cells	268:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	72	theme	nanotheranostic	139:153	arg1	system					155:160	A cancer nanotheranostic system	130:160	A cancer nanotheranostic system	130:160	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	6	73	theme	drug	951:954	arg1	delivery					956:963	drug delivery	951:963	drug delivery	951:963	Afterwards, the potential of the designed nanosystem in targeted imaging, and drug delivery was evaluated using fluorescence microscopy and flow cytometry.
35041881	2	74	theme	MCM	493:495	arg1	Au-Apt					500:505	MCM@CS@Au-Apt	493:505	MCM@CS@Au-Apt	493:505	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	2	74	theme	MCM	493:495	arg1	nanosystem					481:490	the nanosystem	477:490	the nanosystem (MCM@CS@Au-Apt)	477:506	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	8	75	theme	other	1237:1241	arg1	hand					1243:1246	the other hand	1233:1246	the other hand	1233:1246	On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells.
35041881	0	76	theme	theranostic	93:103	arg1	"					56:56	"on/off" optical biosensor and pH-sensitive theranostic platform	49:112	"on/off" optical biosensor and pH-sensitive theranostic platform against cancer	49:127	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	0	76	theme	theranostic	93:103	arg1	platform					105:112	pH-sensitive theranostic platform	80:112	pH-sensitive theranostic platform	80:112	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	9	77	theme	double	1446:1451	arg1	strands					1453:1459	its double strands	1442:1459	its double strands separate under the low pH condition	1442:1495	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	9	77	theme	double	1446:1451	arg1	receptor					1432:1439	the MUC-1 receptor	1422:1439	the MUC-1 receptor	1422:1439	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	9	78	from	release	1518:1524	arg1	state					1569:1573	" state	1567:1573	" state	1567:1573	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	3	79	from	step	578:581	arg1	approved					551:558	approved	551:558	approved	551:558	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	3	79	from	step	578:581	arg1	fabrication					535:545	The nanosystem successful fabrication	509:545	The nanosystem successful fabrication	509:545	The nanosystem successful fabrication was approved at each synthesis step through FTIR, XRD, BET, DLS, FE-SEM, HRTEM, and fluorescence spectroscopy.
35041881	2	80	theme	herbal	339:344	arg1	drug					357:360	an efficient herbal anticancer drug	326:360	an efficient herbal anticancer drug	326:360	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	0	81	theme	silica	11:16	arg1	gold					27:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	9	82	from	fluorescence	1550:1561	arg1	state					1569:1573	" state	1567:1573	" state	1567:1573	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	9	83	theme	separate	1461:1468	arg1	strands					1453:1459	its double strands	1442:1459	its double strands separate under the low pH condition	1442:1495	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	9	83	theme	separate	1461:1468	arg1	receptor					1432:1439	the MUC-1 receptor	1422:1439	the MUC-1 receptor	1422:1439	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	11	84	theme	targeted	1919:1926	arg1	delivery					1937:1944	targeted curcumin delivery	1919:1944	targeted curcumin delivery	1919:1944	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	0	85	theme	chitosan	18:25	arg1	gold					27:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold	0:30	Mesoporous silica@chitosan@gold nanoparticles as "on/off" optical biosensor and pH-sensitive theranostic platform against cancer.
35041881	7	86	theme	"	1209:1209	arg1	biosensor					1219:1227	an "on/off" optical biosensor	1199:1227	an "on/off" optical biosensor	1199:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	10	87	theme	selective	1724:1732	arg1	targeting					1734:1742	its selective targeting	1720:1742	its selective targeting	1720:1742	Based on the toxicity results, this nanosystem had more toxicity toward the MUC-1-positive tumor cells than MUC-1-negative cells, representing its selective targeting.
35041881	8	88	theme	pH-sensitive	1265:1276	arg1	nanoparticles					1287:1299	pH-sensitive chitosan nanoparticles	1265:1299	pH-sensitive chitosan nanoparticles	1265:1299	On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells.
35041881	11	89	theme	smart	1797:1801	arg1	system					1830:1835	a smart anticancer nanotheranostic system	1795:1835	a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery	1795:1944	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	11	89	theme	smart	1797:1801	arg1	nanosystem					1761:1770	this nanosystem	1756:1770	this nanosystem	1756:1770	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	9	90	theme	pH	1484:1485	arg1	condition					1487:1495	the low pH condition	1476:1495	the low pH condition	1476:1495	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	2	91	theme	resulted	439:446	arg1	nanoparticle					448:459	then the resulted nanoparticle	430:459	then the resulted nanoparticle	430:459	Subsequently, curcumin as an efficient herbal anticancer drug was first encapsulated into chitosan-triphosphate nanoparticles and then the resulted nanoparticle was loaded into the nanosystem (MCM@CS@Au-Apt).
35041881	8	92	theme	nanoparticles	1287:1299	arg1	presence					1253:1260	the presence	1249:1260	the presence of pH-sensitive chitosan nanoparticles	1249:1299	On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells.
35041881	7	93	theme	aptamer	1130:1136	arg1	strands					1115:1121	double strands	1108:1121	double strands of DNA aptamer	1108:1136	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	11	94	theme	curcumin	1928:1935	arg1	delivery					1937:1944	targeted curcumin delivery	1919:1944	targeted curcumin delivery	1919:1944	Therefore, this nanosystem could be introduced as a smart anticancer nanotheranostic system for tracing particular biomarkers (MUC-1), non-invasive fluorescence imaging, and targeted curcumin delivery.
35041881	8	95	theme	smart	1309:1313	arg1	nanosystem					1315:1324	smart nanosystem	1309:1324	smart nanosystem to deliver more curcumin into the desired cells	1309:1372	On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells.
35041881	1	96	theme	MUC-1	272:276	arg1	cells					293:297	the MUC-1 positive tumor cells	268:297	the MUC-1 positive tumor cells	268:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	97	theme	gold	213:216	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	97	theme	gold	213:216	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	9	98	theme	drug	1513:1516	arg1	release					1518:1524	the drug release	1509:1524	the drug release	1509:1524	Indeed, when the aptamer specifically binds to the MUC-1 receptor, its double strands separate under the low pH condition, leading to the drug release and the recovery of the fluorescence ("On" state).
35041881	7	99	theme	@	1167:1167	arg1	fluorescence					1171:1182	MCM@CS@Au fluorescence	1161:1182	MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor	1161:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	8	100	theme	more	1337:1340	arg1	curcumin					1342:1349	more curcumin	1337:1349	more curcumin	1337:1349	On the other hand, the presence of pH-sensitive chitosan nanoparticles creates smart nanosystem to deliver more curcumin into the desired cells.
35041881	7	101	theme	strands	1115:1121	arg1	quencher					1148:1155	a quencher	1146:1155	a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor	1146:1227	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	7	101	theme	strands	1115:1121	arg1	transfer					1058:1065	the energy transfer	1047:1065	the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer	1047:1136	It was found that the energy transfer between the base pairs in the hairpin of double strands of DNA aptamer acts as a quencher for MCM@CS@Au fluorescence culminating in an "on/off" optical biosensor.
35041881	1	102	theme	MCM	219:221	arg1	nanosystem					230:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem	186:239	mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells	186:297	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
35041881	1	102	theme	MCM	219:221	arg1	Au					226:227	MCM@CS@Au	219:227	MCM@CS@Au	219:227	A cancer nanotheranostic system was fabricated based on mesoporous silica@chitosan@gold (MCM@CS@Au) nanosystem targeted by aptamer toward the MUC-1 positive tumor cells.
36252894	3	0	theme	environmental	341:353	arg1	dysbiosis					388:396	dysbiosis	388:396	dysbiosis of the intestinal flora and autoimmunity	388:437	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	3	0	theme	environmental	341:353	arg1	factors					355:361	environmental factors	341:361	environmental factors	341:361	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	3	0	theme	environmental	341:353	arg1	susceptibility					372:385	genetic susceptibility	364:385	genetic susceptibility	364:385	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	9	1	theme	diversity	1293:1301	arg1	relationship					1312:1323	its relationship	1308:1323	its relationship with NF-κB p65, STAT3 and IL-6 expression	1308:1365	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	1	theme	diversity	1293:1301	arg1	changes					1249:1255	the changes	1245:1255	the changes of intestinal flora composition and diversity	1245:1301	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	5	2	theme	flora	685:689	arg1	composition					656:666	the composition	652:666	the composition of UC intestinal flora	652:689	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	9	3	theme	flora	1271:1275	arg1	relationship					1312:1323	its relationship	1308:1323	its relationship with NF-κB p65, STAT3 and IL-6 expression	1308:1365	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	3	theme	flora	1271:1275	arg1	changes					1249:1255	the changes	1245:1255	the changes of intestinal flora composition and diversity	1245:1301	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	1	4	theme	Ulcerative	186:195	arg1	disease					236:242	a chronic inflammatory disease	213:242	a chronic inflammatory disease of the intestine	213:259	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	1	4	theme	Ulcerative	186:195	arg1	UC					206:207	UC	206:207	UC	206:207	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	1	4	theme	Ulcerative	186:195	arg1	colitis					197:203	Ulcerative colitis	186:203	Ulcerative colitis (UC)	186:208	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	10	5	theme	NF-κB	1471:1475	arg1	pathway					1487:1493	NF-κB signaling pathway	1471:1493	NF-κB signaling pathway	1471:1493	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	7	6	theme	IL-6	1033:1036	arg1	expression					998:1007	expression	998:1007	expression	998:1007	ELISA and immunohistochemistry were applied to detect the concentration and expression of NF-κB p65, STAT3 and IL-6.
36252894	7	6	theme	IL-6	1033:1036	arg1	concentration					980:992	concentration	980:992	concentration	980:992	ELISA and immunohistochemistry were applied to detect the concentration and expression of NF-κB p65, STAT3 and IL-6.
36252894	9	7	with	relationship	1312:1323	arg1	STAT3					1341:1345	STAT3	1341:1345	STAT3	1341:1345	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	7	with	relationship	1312:1323	arg1	expression					1356:1365	IL-6 expression	1351:1365	IL-6 expression	1351:1365	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	7	with	relationship	1312:1323	arg1	p65					1336:1338	NF-κB p65	1330:1338	NF-κB p65	1330:1338	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	6	8	used	used	767:770	arg2	we					764:765	we	764:765	we	764:765	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	4	9	dep	pathogenesis	486:497	arg1	the					482:484	the	482:484	the	482:484	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	0	10	theme	intestinal	112:121	arg1	composition					129:139	intestinal flora composition	112:139	intestinal flora composition	112:139	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	9	11	theme	treatment	1161:1169	arg1	effect					1171:1176	the treatment effect	1157:1176	the treatment effect of FMT on UC mice	1157:1194	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	4	12	from	role	444:447	arg1	progression					503:513	progression	503:513	progression	503:513	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	4	12	from	role	444:447	arg1	pathogenesis					486:497	pathogenesis	486:497	pathogenesis	486:497	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	5	13	theme	More	557:560	arg1	studies					571:577	More and more studies	557:577	More and more studies	557:577	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	10	14	theme	flora	1414:1418	arg1	disorder					1420:1427	intestinal flora disorder	1403:1427	intestinal flora disorder in mice with ulcerative colitis	1403:1459	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	6	15	theme	sodium	791:796	arg1	DSS					799:801	2% Dextran sulfate sodium (DSS)	772:802	2% Dextran sulfate sodium (DSS) for 7 days	772:813	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	0	16	theme	flora	123:127	arg1	composition					129:139	intestinal flora composition	112:139	intestinal flora composition	112:139	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	0	17	from	inflammation	53:64	arg1	mice					69:72	mice	69:72	mice with ulcerative colitis	69:96	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	9	18	theme	potential	1212:1220	arg1	mechanism					1222:1230	its potential mechanism	1208:1230	its potential mechanism	1208:1230	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	5	19	theme	microbiota	605:614	arg1	FMT					633:635	FMT	633:635	FMT	633:635	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	5	19	theme	microbiota	605:614	arg1	transplantation					616:630	fecal microbiota transplantation	599:630	fecal microbiota transplantation (FMT)	599:636	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	10	20	theme	UC	1548:1549	arg1	mice					1551:1554	UC mice	1548:1554	UC mice	1548:1554	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	0	21	theme	microbiota	6:15	arg1	transplantation					17:31	Fecal microbiota transplantation	0:31	Fecal microbiota transplantation	0:31	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	4	22	theme	UC	518:519	arg1	progression					503:513	progression	503:513	progression	503:513	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	4	22	theme	UC	518:519	arg1	pathogenesis					486:497	pathogenesis	486:497	pathogenesis	486:497	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	5	23	theme	UC	671:672	arg1	flora					685:689	UC intestinal flora	671:689	UC intestinal flora	671:689	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	6	24	theme	Dextran	775:781	arg1	DSS					799:801	2% Dextran sulfate sodium (DSS)	772:802	2% Dextran sulfate sodium (DSS) for 7 days	772:813	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	7	25	theme	NF-κB	1012:1016	arg1	p65					1018:1020	NF-κB p65	1012:1020	NF-κB p65	1012:1020	ELISA and immunohistochemistry were applied to detect the concentration and expression of NF-κB p65, STAT3 and IL-6.
36252894	0	26	theme	Fecal	0:4	arg1	transplantation					17:31	Fecal microbiota transplantation	0:31	Fecal microbiota transplantation	0:31	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	5	27	theme	intestinal	674:683	arg1	flora					685:689	UC intestinal flora	671:689	UC intestinal flora	671:689	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	6	28	theme	%	773:773	arg1	DSS					799:801	2% Dextran sulfate sodium (DSS)	772:802	2% Dextran sulfate sodium (DSS) for 7 days	772:813	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	10	29	theme	signaling	1477:1485	arg1	pathway					1487:1493	NF-κB signaling pathway	1471:1493	NF-κB signaling pathway	1471:1493	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	6	30	from	model	839:843	arg1	mice					848:851	mice	848:851	mice	848:851	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	5	31	theme	new	718:720	arg1	strategy					722:729	a new strategy	716:729	a new strategy for UC treatment	716:746	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	5	31	theme	new	718:720	arg1	it					695:696	it	695:696	it	695:696	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	3	32	theme	intestinal	405:414	arg1	flora					416:420	the intestinal flora	401:420	the intestinal flora	401:420	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	7	33	theme	p65	1018:1020	arg1	expression					998:1007	expression	998:1007	expression	998:1007	ELISA and immunohistochemistry were applied to detect the concentration and expression of NF-κB p65, STAT3 and IL-6.
36252894	7	33	theme	p65	1018:1020	arg1	concentration					980:992	concentration	980:992	concentration	980:992	ELISA and immunohistochemistry were applied to detect the concentration and expression of NF-κB p65, STAT3 and IL-6.
36252894	9	34	theme	IL-6	1351:1354	arg1	expression					1356:1365	IL-6 expression	1351:1365	IL-6 expression	1351:1365	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	35	theme	FMT	1181:1183	arg1	effect					1171:1176	the treatment effect	1157:1176	the treatment effect of FMT on UC mice	1157:1194	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	1	36	theme	chronic	215:221	arg1	colitis					197:203	Ulcerative colitis	186:203	Ulcerative colitis (UC)	186:208	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	1	36	theme	chronic	215:221	arg1	disease					236:242	a chronic inflammatory disease	213:242	a chronic inflammatory disease of the intestine	213:259	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	0	37	theme	signaling	167:175	arg1	pathway					177:183	NF-kB signaling pathway	161:183	NF-kB signaling pathway	161:183	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	6	38	theme	colitis	831:837	arg1	model					839:843	acute colitis model	825:843	acute colitis model in mice	825:851	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	1	39	theme	inflammatory	223:234	arg1	colitis					197:203	Ulcerative colitis	186:203	Ulcerative colitis (UC)	186:208	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	1	39	theme	inflammatory	223:234	arg1	disease					236:242	a chronic inflammatory disease	213:242	a chronic inflammatory disease of the intestine	213:259	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	0	40	theme	intestinal	42:51	arg1	inflammation					53:64	intestinal inflammation	42:64	intestinal inflammation in mice with ulcerative colitis	42:96	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	0	41	theme	NF-kB	161:165	arg1	pathway					177:183	NF-kB signaling pathway	161:183	NF-kB signaling pathway	161:183	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	6	42	theme	acute	825:829	arg1	model					839:843	acute colitis model	825:843	acute colitis model in mice	825:851	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	3	43	theme	autoimmunity	426:437	arg1	dysbiosis					388:396	dysbiosis	388:396	dysbiosis of the intestinal flora and autoimmunity	388:437	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	3	43	theme	autoimmunity	426:437	arg1	factors					355:361	environmental factors	341:361	environmental factors	341:361	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	8	44	theme	flora	1129:1133	arg1	composition					1103:1113	the composition	1099:1113	the composition of intestinal flora	1099:1133	16SrRNA high-throughput sequencing was performed to explore the composition of intestinal flora.
36252894	10	45	theme	intestinal	1521:1530	arg1	inflammation					1532:1543	intestinal inflammation	1521:1543	intestinal inflammation in UC mice	1521:1554	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	9	46	theme	intestinal	1260:1269	arg1	flora					1271:1275	intestinal flora composition and diversity	1260:1301	flora	1271:1275	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	47	from	effect	1171:1176	arg1	mice					1191:1194	UC mice	1188:1194	UC mice	1188:1194	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	8	48	theme	16SrRNA	1039:1045	arg1	sequencing					1063:1072	16SrRNA high-throughput sequencing	1039:1072	16SrRNA high-throughput sequencing	1039:1072	16SrRNA high-throughput sequencing was performed to explore the composition of intestinal flora.
36252894	7	49	theme	STAT3	1023:1027	arg1	expression					998:1007	expression	998:1007	expression	998:1007	ELISA and immunohistochemistry were applied to detect the concentration and expression of NF-κB p65, STAT3 and IL-6.
36252894	7	49	theme	STAT3	1023:1027	arg1	concentration					980:992	concentration	980:992	concentration	980:992	ELISA and immunohistochemistry were applied to detect the concentration and expression of NF-κB p65, STAT3 and IL-6.
36252894	4	50	theme	intestinal	452:461	arg1	disorders					469:477	intestinal flora disorders	452:477	intestinal flora disorders	452:477	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	10	51	theme	intestinal	1403:1412	arg1	flora					1414:1418	intestinal flora	1403:1418	intestinal flora disorder in mice with ulcerative colitis	1403:1459	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	9	52	theme	UC	1188:1189	arg1	mice					1191:1194	UC mice	1188:1194	UC mice	1188:1194	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	10	53	from	inflammation	1532:1543	arg1	mice					1551:1554	UC mice	1548:1554	UC mice	1548:1554	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	9	54	dep	flora	1271:1275	arg1	composition					1277:1287	composition	1277:1287	composition	1277:1287	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	8	55	theme	high-throughput	1047:1061	arg1	sequencing					1063:1072	16SrRNA high-throughput sequencing	1039:1072	16SrRNA high-throughput sequencing	1039:1072	16SrRNA high-throughput sequencing was performed to explore the composition of intestinal flora.
36252894	6	56	theme	sulfate	783:789	arg1	DSS					799:801	2% Dextran sulfate sodium (DSS)	772:802	2% Dextran sulfate sodium (DSS) for 7 days	772:813	In this study, we used 2% Dextran sulfate sodium (DSS) for 7 days to induce acute colitis model in mice, and interfere with FMT and Enterotoxigenic Escherichia coli (ETEC).
36252894	5	57	theme	fecal	599:603	arg1	FMT					633:635	FMT	633:635	FMT	633:635	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	5	57	theme	fecal	599:603	arg1	transplantation					616:630	fecal microbiota transplantation	599:630	fecal microbiota transplantation (FMT)	599:636	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	9	58	theme	NF-κB	1330:1334	arg1	p65					1336:1338	NF-κB p65	1330:1338	NF-κB p65	1330:1338	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	10	59	theme	ulcerative	1442:1451	arg1	colitis					1453:1459	ulcerative colitis	1442:1459	ulcerative colitis	1442:1459	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	4	60	theme	disorders	469:477	arg1	role					444:447	The role	440:447	The role of intestinal flora disorders in the pathogenesis and progression of UC	440:519	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	0	61	with	mice	69:72	arg1	colitis					90:96	ulcerative colitis	79:96	ulcerative colitis	79:96	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	9	62	with	changes	1249:1255	arg1	STAT3					1341:1345	STAT3	1341:1345	STAT3	1341:1345	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	62	with	changes	1249:1255	arg1	expression					1356:1365	IL-6 expression	1351:1365	IL-6 expression	1351:1365	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	9	62	with	changes	1249:1255	arg1	p65					1336:1338	NF-κB p65	1330:1338	NF-κB p65	1330:1338	The aim was to study the treatment effect of FMT on UC mice and explore its potential mechanism by observing the changes of intestinal flora composition and diversity, and its relationship with NF-κB p65, STAT3 and IL-6 expression.
36252894	10	63	from	disorder	1420:1427	arg1	mice					1432:1435	mice	1432:1435	mice with ulcerative colitis	1432:1459	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	0	64	theme	ulcerative	79:88	arg1	colitis					90:96	ulcerative colitis	79:96	ulcerative colitis	79:96	Fecal microbiota transplantation improves intestinal inflammation in mice with ulcerative colitis by modulating intestinal flora composition and down-regulating NF-kB signaling pathway.
36252894	4	65	theme	flora	463:467	arg1	disorders					469:477	intestinal flora disorders	452:477	intestinal flora disorders	452:477	The role of intestinal flora disorders in the pathogenesis and progression of UC is becoming increasingly prominent.
36252894	10	66	with	mice	1432:1435	arg1	colitis					1453:1459	ulcerative colitis	1442:1459	ulcerative colitis	1442:1459	We conclude that FMT could improve intestinal flora disorder in mice with ulcerative colitis, regulate NF-κB signaling pathway, and significantly reduce intestinal inflammation in UC mice.
36252894	1	67	theme	intestine	251:259	arg1	colitis					197:203	Ulcerative colitis	186:203	Ulcerative colitis (UC)	186:208	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	1	67	theme	intestine	251:259	arg1	disease					236:242	a chronic inflammatory disease	213:242	a chronic inflammatory disease of the intestine	213:259	Ulcerative colitis (UC) is a chronic inflammatory disease of the intestine.
36252894	3	68	theme	genetic	364:370	arg1	factors					355:361	environmental factors	341:361	environmental factors	341:361	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	3	68	theme	genetic	364:370	arg1	susceptibility					372:385	genetic susceptibility	364:385	genetic susceptibility	364:385	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	5	69	theme	more	566:569	arg1	studies					571:577	More and more studies	557:577	More and more studies	557:577	More and more studies have confirmed that fecal microbiota transplantation (FMT) could reshape the composition of UC intestinal flora and it is expected to be a new strategy for UC treatment.
36252894	3	70	theme	flora	416:420	arg1	dysbiosis					388:396	dysbiosis	388:396	dysbiosis of the intestinal flora and autoimmunity	388:437	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	3	70	theme	flora	416:420	arg1	factors					355:361	environmental factors	341:361	environmental factors	341:361	The pathogenesis is mainly associated with environmental factors, genetic susceptibility, dysbiosis of the intestinal flora and autoimmunity.
36252894	8	71	theme	intestinal	1118:1127	arg1	flora					1129:1133	intestinal flora	1118:1133	intestinal flora	1118:1133	16SrRNA high-throughput sequencing was performed to explore the composition of intestinal flora.
36807933	9	0	theme	NAFLD	1689:1693	arg1	progression					1703:1713	NAFLD disease progression	1689:1713	NAFLD disease progression	1689:1713	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	3	1	from	signatures	508:517	arg1	NASH					531:534	NASH	531:534	NASH	531:534	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	3	1	from	signatures	508:517	arg1	NAFLD					522:526	NAFLD	522:526	NAFLD	522:526	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	8	2	from	differences	1379:1389	arg1	pathways					1411:1418	LPS biosynthesis pathways	1394:1418	LPS biosynthesis pathways	1394:1418	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	5	3	dep	evaluated	818:826	arg1	11					905:906	11	905:906	11	905:906	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	3	dep	evaluated	818:826	arg1	controls					884:891	11 non-alcoholic fatty liver controls	855:891	11 non-alcoholic fatty liver controls (non-NAFL)	855:902	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	3	dep	evaluated	818:826	arg1	23					930:931	23	930:931	23	930:931	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	3	dep	evaluated	818:826	arg1	non-NAFL					894:901	non-NAFL	894:901	non-NAFL	894:901	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	3	4	from	NAFLD	522:526	arg1	comparisons					483:493	comparisons	483:493	comparisons of microbial signatures in NAFLD or NASH	483:534	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	3	5	theme	discrepant	550:559	arg1	results					561:567	discrepant results	550:567	discrepant results	550:567	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	6	6	theme	fatty	1075:1079	arg1	liver					1081:1085	fatty liver	1075:1085	fatty liver	1075:1085	RESULTS Our study showed that Parabacteroides distasonis and Alistipes putredenis were enriched in fatty liver but not in NASH patients.
36807933	4	7	theme	gut	655:657	arg1	composition					670:680	the gut metagenome composition	651:680	the gut metagenome composition of patients with fatty liver disease	651:717	Thus, we aimed to characterize the gut metagenome composition of patients with fatty liver disease.
36807933	4	8	with	patients	685:692	arg1	disease					711:717	fatty liver disease	699:717	fatty liver disease	699:717	Thus, we aimed to characterize the gut metagenome composition of patients with fatty liver disease.
36807933	9	9	theme	intestinal	1742:1751	arg1	permeability					1753:1764	higher intestinal permeability	1735:1764	higher intestinal permeability	1735:1764	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	7	10	dep	Prevotella	1246:1255	arg1	copri					1257:1261	copri	1257:1261	copri	1257:1261	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	8	11	theme	higher	1468:1473	arg1	levels					1487:1492	higher circulating levels	1468:1492	higher circulating levels of LPS	1468:1499	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	5	12	theme	METHODS	720:726	arg1	microbiome					732:741	METHODS Gut microbiome	720:741	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD	720:812	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	3	13	theme	environmental	597:609	arg1	factors					611:617	ethnic and environmental factors	586:617	ethnic and environmental factors	586:617	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	5	14	theme	fatty	872:876	arg1	controls					884:891	11 non-alcoholic fatty liver controls	855:891	11 non-alcoholic fatty liver controls (non-NAFL)	855:902	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	14	theme	fatty	872:876	arg1	non-NAFL					894:901	non-NAFL	894:901	non-NAFL	894:901	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	3	15	theme	ethnic	586:591	arg1	factors					611:617	ethnic and environmental factors	586:617	ethnic and environmental factors	586:617	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	9	16	theme	butyrate	1789:1796	arg1	production					1798:1807	butyrate production	1789:1807	butyrate production	1789:1807	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	8	17	theme	LPS	1497:1499	arg1	levels					1487:1492	higher circulating levels	1468:1492	higher circulating levels of LPS	1468:1499	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	8	17	theme	LPS	1497:1499	arg1	abundance					1513:1521	a lower abundance	1505:1521	a lower abundance of pathways encoding butyrate production	1505:1562	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	9	18	theme	dominant	1622:1629	arg1	community					1641:1649	a Prevotella-copri dominant bacterial community	1603:1649	a Prevotella-copri dominant bacterial community	1603:1649	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	0	19	from	steatohepatitis	107:121	arg1	subjects					126:133	subjects	126:133	subjects with obesity	126:146	A metagenomic study identifies a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity.
36807933	4	20	theme	patients	685:692	arg1	composition					670:680	the gut metagenome composition	651:680	the gut metagenome composition of patients with fatty liver disease	651:717	Thus, we aimed to characterize the gut metagenome composition of patients with fatty liver disease.
36807933	5	21	theme	patients	768:775	arg1	microbiome					732:741	METHODS Gut microbiome	720:741	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD	720:812	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	1	22	theme	Non-alcoholic	168:180	arg1	NAFLD					203:207	NAFLD	203:207	NAFLD	203:207	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	22	theme	Non-alcoholic	168:180	arg1	disease					194:200	Non-alcoholic fatty liver disease	168:200	Non-alcoholic fatty liver disease (NAFLD)	168:208	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	6	23	dep	RESULTS	976:982	arg1	showed					994:999	showed	994:999	showed that Parabacteroides distasonis and Alistipes putredenis were enriched in fatty liver but not in NASH patients	994:1110	RESULTS Our study showed that Parabacteroides distasonis and Alistipes putredenis were enriched in fatty liver but not in NASH patients.
36807933	4	24	theme	fatty	699:703	arg1	disease					711:717	fatty liver disease	699:717	fatty liver disease	699:717	Thus, we aimed to characterize the gut metagenome composition of patients with fatty liver disease.
36807933	1	25	theme	liver	188:192	arg1	NAFLD					203:207	NAFLD	203:207	NAFLD	203:207	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	25	theme	liver	188:192	arg1	disease					194:200	Non-alcoholic fatty liver disease	168:200	Non-alcoholic fatty liver disease (NAFLD)	168:208	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	8	26	theme	pathways	1526:1533	arg1	levels					1487:1492	higher circulating levels	1468:1492	higher circulating levels of LPS	1468:1499	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	8	26	theme	pathways	1526:1533	arg1	abundance					1513:1521	a lower abundance	1505:1521	a lower abundance of pathways encoding butyrate production	1505:1562	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	1	27	dep	BACKGROUND	149:158	arg1	NAFLD					203:207	NAFLD	203:207	NAFLD	203:207	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	27	dep	BACKGROUND	149:158	arg1	disease					194:200	Non-alcoholic fatty liver disease	168:200	Non-alcoholic fatty liver disease (NAFLD)	168:208	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	2	28	theme	gut	292:294	arg1	microbiota					296:305	the gut microbiota	288:305	the gut microbiota	288:305	Increasing evidence indicates that the gut microbiota can play an important role in the pathophysiology of NAFLD.
36807933	8	29	theme	butyrate	1544:1551	arg1	production					1553:1562	butyrate production	1544:1562	butyrate production	1544:1562	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	3	30	theme	microbiome	433:442	arg1	profiles					444:451	gut microbiome profiles	429:451	gut microbiome profiles	429:451	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	9	31	theme	Prevotella-copri	1605:1620	arg1	community					1641:1649	a Prevotella-copri dominant bacterial community	1603:1649	a Prevotella-copri dominant bacterial community	1603:1649	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	7	32	theme	NASH	1329:1332	arg1	risk					1310:1313	a greater risk	1300:1313	a greater risk of developing NASH	1300:1332	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	8	33	theme	LPS	1394:1396	arg1	pathways					1411:1418	LPS biosynthesis pathways	1394:1418	LPS biosynthesis pathways	1394:1418	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	9	34	attach	linked	1725:1730	arg2	risk					1680:1683	a greater risk	1670:1683	a greater risk	1670:1683	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	9	34	attach	linked	1725:1730	arg1	permeability					1753:1764	higher intestinal permeability	1735:1764	higher intestinal permeability	1735:1764	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	9	34	attach	linked	1725:1730	arg1	capacity					1776:1783	lower capacity	1770:1783	lower capacity for butyrate production	1770:1807	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	7	35	theme	microbial	1161:1169	arg1	profiles					1171:1178	microbial profiles	1161:1178	microbial profiles	1161:1178	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	9	36	theme	bacterial	1631:1639	arg1	community					1641:1649	a Prevotella-copri dominant bacterial community	1603:1649	a Prevotella-copri dominant bacterial community	1603:1649	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	3	37	theme	predictive	409:418	arg1	value					420:424	the predictive value	405:424	the predictive value of gut microbiome profiles in NAFLD progression	405:472	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	5	38	theme	biopsy-proven	794:806	arg1	NAFLD					808:812	biopsy-proven NAFLD	794:812	biopsy-proven NAFLD	794:812	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	7	39	theme	greater	1302:1308	arg1	risk					1310:1313	a greater risk	1300:1313	a greater risk of developing NASH	1300:1332	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	7	40	theme	hierarchical	1127:1138	arg1	analysis					1151:1158	a hierarchical clustering analysis	1125:1158	a hierarchical clustering analysis	1125:1158	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	8	41	theme	Prevotella-dominant	1435:1453	arg1	subjects					1455:1462	Prevotella-dominant subjects	1435:1462	Prevotella-dominant subjects	1435:1462	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	9	42	theme	greater	1672:1678	arg1	risk					1680:1683	a greater risk	1670:1683	a greater risk	1670:1683	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	1	43	theme	liver	238:242	arg1	disease					244:250	liver disease	238:250	liver disease	238:250	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	7	44	theme	dominant	1263:1270	arg1	cluster					1272:1278	a Prevotella copri dominant cluster	1244:1278	a Prevotella copri dominant cluster	1244:1278	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	3	45	theme	signatures	508:517	arg1	comparisons					483:493	comparisons	483:493	comparisons of microbial signatures in NAFLD or NASH	483:534	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	9	46	theme	disease	1695:1701	arg1	progression					1703:1713	NAFLD disease progression	1689:1713	NAFLD disease progression	1689:1713	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	7	47	theme	Prevotella	1246:1255	arg1	cluster					1272:1278	a Prevotella copri dominant cluster	1244:1278	a Prevotella copri dominant cluster	1244:1278	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	0	48	with	subjects	126:133	arg1	obesity					140:146	obesity	140:146	obesity	140:146	A metagenomic study identifies a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity.
36807933	8	49	theme	Functional	1335:1344	arg1	analyses					1346:1353	Functional analyses	1335:1353	Functional analyses	1335:1353	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	5	50	theme	liver	878:882	arg1	controls					884:891	11 non-alcoholic fatty liver controls	855:891	11 non-alcoholic fatty liver controls (non-NAFL)	855:902	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	50	theme	liver	878:882	arg1	non-NAFL					894:901	non-NAFL	894:901	non-NAFL	894:901	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	0	51	theme	enriched	50:57	arg1	profile					69:75	a Prevotella-copri enriched microbial profile	31:75	a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity	31:146	A metagenomic study identifies a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity.
36807933	3	52	from	comparisons	483:493	arg1	NASH					531:534	NASH	531:534	NASH	531:534	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	3	52	from	comparisons	483:493	arg1	NAFLD					522:526	NAFLD	522:526	NAFLD	522:526	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	2	53	theme	important	319:327	arg1	role					329:332	an important role	316:332	an important role	316:332	Increasing evidence indicates that the gut microbiota can play an important role in the pathophysiology of NAFLD.
36807933	9	54	theme	higher	1735:1740	arg1	permeability					1753:1764	higher intestinal permeability	1735:1764	higher intestinal permeability	1735:1764	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	4	55	theme	metagenome	659:668	arg1	composition					670:680	the gut metagenome composition	651:680	the gut metagenome composition of patients with fatty liver disease	651:717	Thus, we aimed to characterize the gut metagenome composition of patients with fatty liver disease.
36807933	8	56	theme	circulating	1475:1485	arg1	levels					1487:1492	higher circulating levels	1468:1492	higher circulating levels of LPS	1468:1499	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	5	57	theme	non-alcoholic	858:870	arg1	controls					884:891	11 non-alcoholic fatty liver controls	855:891	11 non-alcoholic fatty liver controls (non-NAFL)	855:902	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	57	theme	non-alcoholic	858:870	arg1	non-NAFL					894:901	non-NAFL	894:901	non-NAFL	894:901	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	2	58	theme	NAFLD	360:364	arg1	pathophysiology					341:355	the pathophysiology	337:355	the pathophysiology of NAFLD	337:364	Increasing evidence indicates that the gut microbiota can play an important role in the pathophysiology of NAFLD.
36807933	9	59	theme	lower	1770:1774	arg1	capacity					1776:1783	lower capacity	1770:1783	lower capacity for butyrate production	1770:1807	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	5	60	theme	fatty	913:917	arg1	liver					919:923	fatty liver	913:923	fatty liver	913:923	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	4	61	theme	liver	705:709	arg1	disease					711:717	fatty liver disease	699:717	fatty liver disease	699:717	Thus, we aimed to characterize the gut metagenome composition of patients with fatty liver disease.
36807933	8	62	theme	lower	1507:1511	arg1	abundance					1513:1521	a lower abundance	1505:1521	a lower abundance of pathways encoding butyrate production	1505:1562	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	5	63	theme	well-characterized	749:766	arg1	patients					768:775	45 well-characterized patients	746:775	45 well-characterized patients with obesity and biopsy-proven NAFLD	746:812	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	2	64	theme	Increasing	253:262	arg1	evidence					264:271	Increasing evidence	253:271	Increasing evidence	253:271	Increasing evidence indicates that the gut microbiota can play an important role in the pathophysiology of NAFLD.
36807933	5	65	with	patients	768:775	arg1	NAFLD					808:812	biopsy-proven NAFLD	794:812	biopsy-proven NAFLD	794:812	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	65	with	patients	768:775	arg1	obesity					782:788	obesity	782:788	obesity	782:788	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	3	66	from	NASH	531:534	arg1	comparisons					483:493	comparisons	483:493	comparisons of microbial signatures in NAFLD or NASH	483:534	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	0	67	theme	non-alcoholic	93:105	arg1	steatohepatitis					107:121	non-alcoholic steatohepatitis	93:121	non-alcoholic steatohepatitis in subjects with obesity	93:146	A metagenomic study identifies a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity.
36807933	1	68	theme	fatty	182:186	arg1	NAFLD					203:207	NAFLD	203:207	NAFLD	203:207	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	68	theme	fatty	182:186	arg1	disease					194:200	Non-alcoholic fatty liver disease	168:200	Non-alcoholic fatty liver disease (NAFLD)	168:208	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	5	69	theme	Gut	728:730	arg1	microbiome					732:741	METHODS Gut microbiome	720:741	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD	720:812	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	3	70	from	value	420:424	arg1	progression					462:472	NAFLD progression	456:472	NAFLD progression	456:472	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	3	71	theme	profiles	444:451	arg1	value					420:424	the predictive value	405:424	the predictive value of gut microbiome profiles in NAFLD progression	405:472	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	0	72	theme	metagenomic	2:12	arg1	study					14:18	A metagenomic study	0:18	A metagenomic study	0:18	A metagenomic study identifies a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity.
36807933	7	73	theme	developing	1318:1327	arg1	NASH					1329:1332	developing NASH	1318:1332	developing NASH	1318:1332	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	8	74	contain	had	1464:1466	arg2	abundance					1513:1521	a lower abundance	1505:1521	a lower abundance of pathways encoding butyrate production	1505:1562	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	8	74	contain	had	1464:1466	arg1	subjects					1455:1462	Prevotella-dominant subjects	1435:1462	Prevotella-dominant subjects	1435:1462	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	8	74	contain	had	1464:1466	arg2	levels					1487:1492	higher circulating levels	1468:1492	higher circulating levels of LPS	1468:1499	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	9	75	dep	CONCLUSIONS	1565:1575	arg1	suggest					1590:1596	suggest	1590:1596	suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production	1590:1807	CONCLUSIONS Our findings suggest that a Prevotella-copri dominant bacterial community is associated with a greater risk for NAFLD disease progression, probably linked to higher intestinal permeability and lower capacity for butyrate production.
36807933	3	76	theme	gut	429:431	arg1	profiles					444:451	gut microbiome profiles	429:451	gut microbiome profiles	429:451	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	5	77	theme	shot-gun	834:841	arg1	sequencing					843:852	shot-gun sequencing	834:852	shot-gun sequencing	834:852	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	78	theme	non-alcoholic	938:950	arg1	NASH					969:972	NASH	969:972	NASH	969:972	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	5	78	theme	non-alcoholic	938:950	arg1	steatohepatitis					952:966	non-alcoholic steatohepatitis	938:966	non-alcoholic steatohepatitis (NASH)	938:973	METHODS Gut microbiome of 45 well-characterized patients with obesity and biopsy-proven NAFLD was evaluated using shot-gun sequencing: 11 non-alcoholic fatty liver controls (non-NAFL), 11 with fatty liver, and 23 with non-alcoholic steatohepatitis (NASH).
36807933	3	79	theme	several	377:383	arg1	studies					385:391	several studies	377:391	several studies	377:391	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	7	80	theme	clustering	1140:1149	arg1	analysis					1151:1158	a hierarchical clustering analysis	1125:1158	a hierarchical clustering analysis	1125:1158	Notably, in a hierarchical clustering analysis, microbial profiles were differentially distributed among groups, and membership to a Prevotella copri dominant cluster was associated with a greater risk of developing NASH.
36807933	8	81	theme	biosynthesis	1398:1409	arg1	pathways					1411:1418	LPS biosynthesis pathways	1394:1418	LPS biosynthesis pathways	1394:1418	Functional analyses showed that although no differences in LPS biosynthesis pathways were observed, Prevotella-dominant subjects had higher circulating levels of LPS and a lower abundance of pathways encoding butyrate production.
36807933	3	82	theme	NAFLD	456:460	arg1	progression					462:472	NAFLD progression	456:472	NAFLD progression	456:472	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	0	83	theme	microbial	59:67	arg1	profile					69:75	a Prevotella-copri enriched microbial profile	31:75	a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity	31:146	A metagenomic study identifies a Prevotella-copri enriched microbial profile associated with non-alcoholic steatohepatitis in subjects with obesity.
36807933	3	84	theme	microbial	498:506	arg1	signatures					508:517	microbial signatures	498:517	microbial signatures in NAFLD or NASH	498:534	Recently, several studies have tested the predictive value of gut microbiome profiles in NAFLD progression, however comparisons of microbial signatures in NAFLD or NASH have produced discrepant results, possibly due to ethnic and environmental factors.
36807933	6	85	theme	NASH	1098:1101	arg1	patients					1103:1110	NASH patients	1098:1110	NASH patients	1098:1110	RESULTS Our study showed that Parabacteroides distasonis and Alistipes putredenis were enriched in fatty liver but not in NASH patients.
36807933	1	86	theme	common	222:227	arg1	BACKGROUND					149:158	BACKGROUND	149:158	BACKGROUND	149:158	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	86	theme	common	222:227	arg1	cause					229:233	the most common cause	213:233	the most common cause of liver disease	213:250	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	86	theme	common	222:227	arg1	AIM					164:166	AIM	164:166	AIM	164:166	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	87	theme	disease	244:250	arg1	BACKGROUND					149:158	BACKGROUND	149:158	BACKGROUND	149:158	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	87	theme	disease	244:250	arg1	cause					229:233	the most common cause	213:233	the most common cause of liver disease	213:250	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
36807933	1	87	theme	disease	244:250	arg1	AIM					164:166	AIM	164:166	AIM	164:166	BACKGROUND AND AIM Non-alcoholic fatty liver disease (NAFLD) is the most common cause of liver disease.
35850197	14	0	theme	potential	2093:2101	arg1	targets					2113:2119	potential microbial targets	2093:2119	potential microbial targets	2093:2119	These findings may identify potential microbial targets to modulate gut barrier.
35850197	1	1	theme	barrier	225:231	arg1	hemostasis					233:242	gut barrier hemostasis	221:242	gut barrier hemostasis	221:242	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	12	2	theme	gut	1895:1897	arg1	function					1907:1914	gut barrier function	1895:1914	gut barrier function	1895:1914	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	13	3	dep	CONCLUSIONS	1958:1968	arg1	pathways					2003:2010	pathways	2003:2010	pathways	2003:2010	CONCLUSIONS The gut microbiome community and pathways are associated with changes in gut barrier function.
35850197	13	3	dep	CONCLUSIONS	1958:1968	arg1	community					1989:1997	The gut microbiome community	1970:1997	The gut microbiome community	1970:1997	CONCLUSIONS The gut microbiome community and pathways are associated with changes in gut barrier function.
35850197	11	4	theme	Adlercreutzia	1567:1579	arg1	abundance					1648:1656	increased abundance	1638:1656	increased abundance of Colidextribacter	1638:1676	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	11	4	theme	Adlercreutzia	1567:1579	arg1	prevalence					1553:1562	decreased prevalence	1543:1562	decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1	1543:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	8	5	theme	community	1108:1116	arg1	contribution					1118:1129	microbial community contribution	1098:1129	microbial community contribution to barrier function	1098:1149	A random forest classifier algorithm was performed to assess microbial community contribution to barrier function.
35850197	9	6	theme	P =	1343:1345	arg1	1.0e-3					1347:1352	P = 1.0e-3	1343:1352	P = 1.0e-3	1343:1352	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	6	theme	P =	1343:1345	arg1	index					1324:1328	Bray-Curtis dissimilarity index	1298:1328	Bray-Curtis dissimilarity index	1298:1328	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	5	7	theme	sequencing	833:842	arg1	RNA					864:866	sequencing fecal 16S ribosomal RNA	833:866	sequencing fecal 16S ribosomal RNA	833:866	Microbiome composition was assessed by sequencing fecal 16S ribosomal RNA.
35850197	5	8	theme	16S	850:852	arg1	RNA					864:866	sequencing fecal 16S ribosomal RNA	833:866	sequencing fecal 16S ribosomal RNA	833:866	Microbiome composition was assessed by sequencing fecal 16S ribosomal RNA.
35850197	10	9	theme	LMR	1507:1509	arg1	>0.025					1511:1516	an LMR >0.025	1504:1516	an LMR >0.025 (q < 0.05)	1504:1527	When taxa were assessed individually, we identified 8 genera and 52 microbial pathways associated with an LMR >0.025 (q < 0.05).
35850197	10	9	theme	LMR	1507:1509	arg1	<					1521:1521	q < 0.05	1519:1526	q < 0.05	1519:1526	When taxa were assessed individually, we identified 8 genera and 52 microbial pathways associated with an LMR >0.025 (q < 0.05).
35850197	7	10	theme	microbial	1000:1008	arg1	associations					1010:1021	microbial associations	1000:1021	microbial associations with the LMR	1000:1034	A regression model was used to assess microbial associations with the LMR.
35850197	9	11	theme	impaired	1177:1184	arg1	function					1194:1201	impaired barrier function	1177:1201	impaired barrier function (LMR >0.025)	1177:1214	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	11	theme	impaired	1177:1184	arg1	LMR					1204:1206	LMR >0.025	1204:1213	LMR >0.025	1204:1213	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	3	12	theme	Colitis	551:557	arg1	Canada					559:564	Colitis Canada	551:564	Colitis Canada	551:564	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	11	13	theme	decreased	1705:1713	arg1	biosynthesis					1715:1726	decreased biosynthesis	1705:1726	decreased biosynthesis of glutamate, tryptophan, and threonine	1705:1766	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	4	14	theme	Gut	683:685	arg1	function					695:702	Gut barrier function	683:702	Gut barrier function	683:702	Gut barrier function was assessed using the urinary fractional excretion of lactulose-to-mannitol ratio (LMR).
35850197	11	15	theme	increased	1638:1646	arg1	abundance					1648:1656	increased abundance	1638:1656	increased abundance of Colidextribacter	1638:1676	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	7	16	theme	regression	964:973	arg1	model					975:979	A regression model	962:979	A regression model	962:979	A regression model was used to assess microbial associations with the LMR.
35850197	13	17	theme	gut	1974:1976	arg1	community					1989:1997	The gut microbiome community	1970:1997	The gut microbiome community	1970:1997	CONCLUSIONS The gut microbiome community and pathways are associated with changes in gut barrier function.
35850197	3	18	theme	Microbial	588:596	arg1	cohort					608:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort	535:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	535:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	2	19	theme	first-degree	462:473	arg1	relatives					475:483	healthy first-degree relatives	454:483	healthy first-degree relatives of patients with Crohn's disease	454:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	0	20	with	Patients	116:123	arg1	Disease					138:144	Crohn's Disease	130:144	Crohn's Disease	130:144	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	9	21	theme	reduced	1220:1226	arg1	alpha-diversity					1228:1242	reduced alpha-diversity	1220:1242	reduced alpha-diversity (Chao1 index, P = 4.0e-4)	1220:1268	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	3	22	theme	Genetic	566:572	arg1	cohort					608:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort	535:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	535:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	9	23	dep	beta-diversity	1282:1295	arg1	1.0e-3					1347:1352	P = 1.0e-3	1343:1352	P = 1.0e-3	1343:1352	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	23	dep	beta-diversity	1282:1295	arg1	R2 =					1331:1334	R2 =	1331:1334	R2 =	1331:1334	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	23	dep	beta-diversity	1282:1295	arg1	index					1324:1328	Bray-Curtis dissimilarity index	1298:1328	Bray-Curtis dissimilarity index	1298:1328	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	0	24	theme	Barrier	72:78	arg1	Dysfunction					80:90	Gut Barrier Dysfunction	68:90	Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease	68:144	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	1	25	theme	&	158:158	arg1	AIMS					160:163	BACKGROUND & AIMS	147:163	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.	147:294	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	12	26	theme	random	1815:1820	arg1	approach					1829:1836	The random forest approach	1811:1836	The random forest approach	1811:1836	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	12	27	dep	0.63	1939:1942	arg1	P =					1945:1947	P =	1945:1947	P =	1945:1947	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	12	28	dep	associated	1879:1888	arg1	area					1917:1920	area	1917:1920	area under the curve	1917:1936	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	4	29	theme	fractional	735:744	arg1	excretion					746:754	the urinary fractional excretion	723:754	the urinary fractional excretion of lactulose-to-mannitol ratio (LMR)	723:791	Gut barrier function was assessed using the urinary fractional excretion of lactulose-to-mannitol ratio (LMR).
35850197	2	30	theme	microbial	352:360	arg1	composition					362:372	gut microbial composition	348:372	gut microbial composition	348:372	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	9	31	theme	P =	1258:1260	arg1	index					1251:1255	Chao1 index	1245:1255	Chao1 index	1245:1255	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	31	theme	P =	1258:1260	arg1	4.0e-4					1262:1267	P = 4.0e-4	1258:1267	P = 4.0e-4	1258:1267	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	2	32	theme	gut	418:420	arg1	function					430:437	gut barrier function	418:437	gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease	418:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	0	33	theme	Altered	0:6	arg1	Composition					23:33	Altered Gut Microbiome Composition	0:33	Altered Gut Microbiome Composition	0:33	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	9	34	with	Individuals	1160:1170	arg1	function					1194:1201	impaired barrier function	1177:1201	impaired barrier function (LMR >0.025)	1177:1214	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	34	with	Individuals	1160:1170	arg1	LMR					1204:1206	LMR >0.025	1204:1213	LMR >0.025	1204:1213	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	13	35	from	changes	2032:2038	arg1	function					2055:2062	gut barrier function	2043:2062	gut barrier function	2043:2062	CONCLUSIONS The gut microbiome community and pathways are associated with changes in gut barrier function.
35850197	0	36	theme	Microbiome	12:21	arg1	Composition					23:33	Altered Gut Microbiome Composition	0:33	Altered Gut Microbiome Composition	0:33	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	9	37	theme	altered	1274:1280	arg1	beta-diversity					1282:1295	altered beta-diversity	1274:1295	altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3)	1274:1353	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	3	38	dep	METHODS	519:525	arg1	used					530:533	used	530:533	used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	530:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	9	39	dep	alpha-diversity	1228:1242	arg1	index					1251:1255	Chao1 index	1245:1255	Chao1 index	1245:1255	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	39	dep	alpha-diversity	1228:1242	arg1	4.0e-4					1262:1267	P = 4.0e-4	1258:1267	P = 4.0e-4	1258:1267	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	12	40	theme	barrier	1899:1905	arg1	function					1907:1914	gut barrier function	1895:1914	gut barrier function	1895:1914	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	3	41	theme	CCC-GEM	599:605	arg1	cohort					608:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort	535:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	535:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	1	42	dep	AIMS	160:163	arg1	scarce					258:263	scarce	258:263	scarce	258:263	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	1	42	dep	AIMS	160:163	arg1	suggested					193:201	suggested	193:201	has been suggested to play a role in gut barrier hemostasis	184:242	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	9	43	theme	dissimilarity	1310:1322	arg1	1.0e-3					1347:1352	P = 1.0e-3	1343:1352	P = 1.0e-3	1343:1352	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	43	theme	dissimilarity	1310:1322	arg1	R2 =					1331:1334	R2 =	1331:1334	R2 =	1331:1334	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	43	theme	dissimilarity	1310:1322	arg1	index					1324:1328	Bray-Curtis dissimilarity index	1298:1328	Bray-Curtis dissimilarity index	1298:1328	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	12	44	dep	area	1917:1920	arg1	0.63					1939:1942	0.63	1939:1942	0.63	1939:1942	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	3	45	theme	relatives	639:647	arg1	cohort					608:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort	535:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	535:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	13	46	theme	gut	2043:2045	arg1	function					2055:2062	gut barrier function	2043:2062	gut barrier function	2043:2062	CONCLUSIONS The gut microbiome community and pathways are associated with changes in gut barrier function.
35850197	8	47	theme	random	1039:1044	arg1	algorithm					1064:1072	A random forest classifier algorithm	1037:1072	A random forest classifier algorithm	1037:1072	A random forest classifier algorithm was performed to assess microbial community contribution to barrier function.
35850197	10	48	theme	microbial	1469:1477	arg1	pathways					1479:1486	52 microbial pathways	1466:1486	52 microbial pathways associated with an LMR >0.025 (q < 0.05)	1466:1527	When taxa were assessed individually, we identified 8 genera and 52 microbial pathways associated with an LMR >0.025 (q < 0.05).
35850197	3	49	theme	healthy	618:624	arg1	relatives					639:647	healthy first-degree relatives	618:647	healthy first-degree relatives of patients with Crohn's disease	618:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	4	50	theme	ratio	781:785	arg1	excretion					746:754	the urinary fractional excretion	723:754	the urinary fractional excretion of lactulose-to-mannitol ratio (LMR)	723:791	Gut barrier function was assessed using the urinary fractional excretion of lactulose-to-mannitol ratio (LMR).
35850197	8	51	theme	classifier	1053:1062	arg1	algorithm					1064:1072	A random forest classifier algorithm	1037:1072	A random forest classifier algorithm	1037:1072	A random forest classifier algorithm was performed to assess microbial community contribution to barrier function.
35850197	14	52	theme	microbial	2103:2111	arg1	targets					2113:2119	potential microbial targets	2093:2119	potential microbial targets	2093:2119	These findings may identify potential microbial targets to modulate gut barrier.
35850197	11	53	theme	decreased	1543:1551	arg1	prevalence					1553:1562	decreased prevalence	1543:1562	decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1	1543:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	11	54	theme	threonine	1758:1766	arg1	biosynthesis					1715:1726	decreased biosynthesis	1705:1726	decreased biosynthesis of glutamate, tryptophan, and threonine	1705:1766	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	3	55	with	patients	652:659	arg1	disease					674:680	Crohn's disease	666:680	Crohn's disease	666:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	1	56	theme	gut	221:223	arg1	hemostasis					233:242	gut barrier hemostasis	221:242	gut barrier hemostasis	221:242	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	11	57	theme	UCG	1593:1595	arg1	014					1597:1599	Clostridia UCG 014	1582:1599	Clostridia UCG 014	1582:1599	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	3	58	theme	patients	652:659	arg1	relatives					639:647	healthy first-degree relatives	618:647	healthy first-degree relatives of patients with Crohn's disease	618:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	1	59	theme	gut	169:171	arg1	microbiome					173:182	The gut microbiome	165:182	The gut microbiome	165:182	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	8	60	theme	microbial	1098:1106	arg1	contribution					1118:1129	microbial community contribution	1098:1129	microbial community contribution to barrier function	1098:1149	A random forest classifier algorithm was performed to assess microbial community contribution to barrier function.
35850197	12	61	theme	bacterial	1856:1864	arg1	community					1866:1874	the bacterial community	1852:1874	the bacterial community	1852:1874	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	10	62	theme	q	1519:1519	arg1	>0.025					1511:1516	an LMR >0.025	1504:1516	an LMR >0.025 (q < 0.05)	1504:1527	When taxa were assessed individually, we identified 8 genera and 52 microbial pathways associated with an LMR >0.025 (q < 0.05).
35850197	10	62	theme	q	1519:1519	arg1	<					1521:1521	q < 0.05	1519:1526	q < 0.05	1519:1526	When taxa were assessed individually, we identified 8 genera and 52 microbial pathways associated with an LMR >0.025 (q < 0.05).
35850197	5	63	theme	fecal	844:848	arg1	RNA					864:866	sequencing fecal 16S ribosomal RNA	833:866	sequencing fecal 16S ribosomal RNA	833:866	Microbiome composition was assessed by sequencing fecal 16S ribosomal RNA.
35850197	7	64	used	used	985:988	arg2	model					975:979	A regression model	962:979	A regression model	962:979	A regression model was used to assess microbial associations with the LMR.
35850197	11	65	theme	sensu	1618:1622	arg1	stricto					1624:1630	Clostridium sensu stricto 1	1606:1632	Clostridium sensu stricto 1	1606:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	5	66	theme	Microbiome	794:803	arg1	composition					805:815	Microbiome composition	794:815	Microbiome composition	794:815	Microbiome composition was assessed by sequencing fecal 16S ribosomal RNA.
35850197	8	67	theme	barrier	1134:1140	arg1	function					1142:1149	barrier function	1134:1149	barrier function	1134:1149	A random forest classifier algorithm was performed to assess microbial community contribution to barrier function.
35850197	0	68	from	Dysfunction	80:90	arg1	Relatives					103:111	Healthy Relatives	95:111	Healthy Relatives of Patients With Crohn's Disease	95:144	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	9	69	theme	barrier	1186:1192	arg1	function					1194:1201	impaired barrier function	1177:1201	impaired barrier function (LMR >0.025)	1177:1214	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	69	theme	barrier	1186:1192	arg1	LMR					1204:1206	LMR >0.025	1204:1213	LMR >0.025	1204:1213	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	3	70	used	used	530:533	arg2	We					527:528	We	527:528	We	527:528	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	11	71	theme	glutamate	1731:1739	arg1	biosynthesis					1715:1726	decreased biosynthesis	1705:1726	decreased biosynthesis of glutamate, tryptophan, and threonine	1705:1766	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	3	72	theme	Crohn	539:543	arg1	cohort					608:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort	535:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	535:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	6	73	theme	discovery	899:907	arg1	cohort					909:914	a discovery cohort	897:914	a discovery cohort (n = 2472)	897:925	The cohort was divided into a discovery cohort (n = 2472) and a validation cohort (n = 655).
35850197	6	73	theme	discovery	899:907	arg1	n =					917:919	n = 2472	917:924	n = 2472	917:924	The cohort was divided into a discovery cohort (n = 2472) and a validation cohort (n = 655).
35850197	11	74	theme	stricto	1624:1630	arg1	abundance					1648:1656	increased abundance	1638:1656	increased abundance of Colidextribacter	1638:1676	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	11	74	theme	stricto	1624:1630	arg1	prevalence					1553:1562	decreased prevalence	1543:1562	decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1	1543:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	3	75	theme	Environmental	574:586	arg1	cohort					608:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort	535:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	535:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	0	76	theme	Gut	68:70	arg1	Dysfunction					80:90	Gut Barrier Dysfunction	68:90	Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease	68:144	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	13	77	theme	microbiome	1978:1987	arg1	community					1989:1997	The gut microbiome community	1970:1997	The gut microbiome community	1970:1997	CONCLUSIONS The gut microbiome community and pathways are associated with changes in gut barrier function.
35850197	3	78	theme	Canada	559:564	arg1	cohort					608:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort	535:613	the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease	535:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	9	79	theme	LMR	1389:1391	arg1	≤0.025					1393:1398	an LMR ≤0.025	1386:1398	an LMR ≤0.025	1386:1398	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	5	80	theme	ribosomal	854:862	arg1	RNA					864:866	sequencing fecal 16S ribosomal RNA	833:866	sequencing fecal 16S ribosomal RNA	833:866	Microbiome composition was assessed by sequencing fecal 16S ribosomal RNA.
35850197	2	81	theme	healthy	454:460	arg1	relatives					475:483	healthy first-degree relatives	454:483	healthy first-degree relatives of patients with Crohn's disease	454:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	9	82	contain	had	1216:1218	arg2	beta-diversity					1282:1295	altered beta-diversity	1274:1295	altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3)	1274:1353	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	82	contain	had	1216:1218	arg1	Individuals					1160:1170	Individuals	1160:1170	Individuals with impaired barrier function (LMR >0.025)	1160:1214	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	82	contain	had	1216:1218	arg2	alpha-diversity					1228:1242	reduced alpha-diversity	1220:1242	reduced alpha-diversity (Chao1 index, P = 4.0e-4)	1220:1268	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	1	83	theme	BACKGROUND	147:156	arg1	AIMS					160:163	BACKGROUND & AIMS	147:163	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.	147:294	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	0	84	theme	Patients	116:123	arg1	Relatives					103:111	Healthy Relatives	95:111	Healthy Relatives of Patients With Crohn's Disease	95:144	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	0	85	theme	Healthy	95:101	arg1	Relatives					103:111	Healthy Relatives	95:111	Healthy Relatives of Patients With Crohn's Disease	95:144	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	9	86	theme	Chao1	1245:1249	arg1	index					1251:1255	Chao1 index	1245:1255	Chao1 index	1245:1255	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	86	theme	Chao1	1245:1249	arg1	4.0e-4					1262:1267	P = 4.0e-4	1258:1267	P = 4.0e-4	1258:1267	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	12	87	theme	forest	1822:1827	arg1	approach					1829:1836	The random forest approach	1811:1836	The random forest approach	1811:1836	The random forest approach revealed that the bacterial community is associated with gut barrier function (area under the curve, 0.63; P = 1.4e-6).
35850197	9	88	dep	RESULTS	1152:1158	arg1	had					1216:1218	had	1216:1218	had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025	1216:1398	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	6	89	theme	validation	933:942	arg1	cohort					944:949	a validation cohort	931:949	a validation cohort (n = 655)	931:959	The cohort was divided into a discovery cohort (n = 2472) and a validation cohort (n = 655).
35850197	6	89	theme	validation	933:942	arg1	655					956:958	n = 655	952:958	n = 655	952:958	The cohort was divided into a discovery cohort (n = 2472) and a validation cohort (n = 655).
35850197	2	90	theme	patients	488:495	arg1	relatives					475:483	healthy first-degree relatives	454:483	healthy first-degree relatives of patients with Crohn's disease	454:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	2	91	from	function	430:437	arg1	cohort					444:449	a cohort	442:449	a cohort of healthy first-degree relatives of patients with Crohn's disease	442:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	11	92	theme	Colidextribacter	1661:1676	arg1	abundance					1648:1656	increased abundance	1638:1656	increased abundance of Colidextribacter	1638:1676	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	11	92	theme	Colidextribacter	1661:1676	arg1	prevalence					1553:1562	decreased prevalence	1543:1562	decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1	1543:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	2	93	theme	relatives	475:483	arg1	cohort					444:449	a cohort	442:449	a cohort of healthy first-degree relatives of patients with Crohn's disease	442:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	4	94	theme	urinary	727:733	arg1	excretion					746:754	the urinary fractional excretion	723:754	the urinary fractional excretion of lactulose-to-mannitol ratio (LMR)	723:791	Gut barrier function was assessed using the urinary fractional excretion of lactulose-to-mannitol ratio (LMR).
35850197	2	95	theme	gut	348:350	arg1	composition					362:372	gut microbial composition	348:372	gut microbial composition	348:372	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	0	96	theme	Gut	8:10	arg1	Composition					23:33	Altered Gut Microbiome Composition	0:33	Altered Gut Microbiome Composition	0:33	Altered Gut Microbiome Composition and Function Are Associated With Gut Barrier Dysfunction in Healthy Relatives of Patients With Crohn's Disease.
35850197	11	97	theme	Clostridium	1606:1616	arg1	stricto					1624:1630	Clostridium sensu stricto 1	1606:1632	Clostridium sensu stricto 1	1606:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	4	98	theme	barrier	687:693	arg1	function					695:702	Gut barrier function	683:702	Gut barrier function	683:702	Gut barrier function was assessed using the urinary fractional excretion of lactulose-to-mannitol ratio (LMR).
35850197	11	99	dep	genera	1535:1540	arg1	abundance					1648:1656	increased abundance	1638:1656	increased abundance of Colidextribacter	1638:1676	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	11	99	dep	genera	1535:1540	arg1	prevalence					1553:1562	decreased prevalence	1543:1562	decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1	1543:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	2	100	with	patients	488:495	arg1	disease					510:516	Crohn's disease	502:516	Crohn's disease	502:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	11	101	theme	014	1597:1599	arg1	abundance					1648:1656	increased abundance	1638:1656	increased abundance of Colidextribacter	1638:1676	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	11	101	theme	014	1597:1599	arg1	prevalence					1553:1562	decreased prevalence	1543:1562	decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1	1543:1632	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	14	102	theme	gut	2133:2135	arg1	barrier					2137:2143	gut barrier	2133:2143	gut barrier	2133:2143	These findings may identify potential microbial targets to modulate gut barrier.
35850197	2	103	theme	functional	378:387	arg1	pathways					389:396	functional pathways	378:396	functional pathways	378:396	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	11	104	theme	validation	1792:1801	arg1	cohort					1803:1808	the validation cohort	1788:1808	the validation cohort	1788:1808	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	11	105	theme	Clostridia	1582:1591	arg1	014					1597:1599	Clostridia UCG 014	1582:1599	Clostridia UCG 014	1582:1599	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	9	106	theme	Bray-Curtis	1298:1308	arg1	1.0e-3					1347:1352	P = 1.0e-3	1343:1352	P = 1.0e-3	1343:1352	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	106	theme	Bray-Curtis	1298:1308	arg1	R2 =					1331:1334	R2 =	1331:1334	R2 =	1331:1334	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	9	106	theme	Bray-Curtis	1298:1308	arg1	index					1324:1328	Bray-Curtis dissimilarity index	1298:1328	Bray-Curtis dissimilarity index	1298:1328	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	2	107	from	alterations	333:343	arg1	composition					362:372	gut microbial composition	348:372	gut microbial composition	348:372	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	2	107	from	alterations	333:343	arg1	pathways					389:396	functional pathways	378:396	functional pathways	378:396	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	7	108	with	associations	1010:1021	arg1	LMR					1032:1034	the LMR	1028:1034	the LMR	1028:1034	A regression model was used to assess microbial associations with the LMR.
35850197	2	109	theme	barrier	422:428	arg1	function					430:437	gut barrier function	418:437	gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease	418:516	We therefore aimed to assess whether alterations in gut microbial composition and functional pathways are associated with gut barrier function in a cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	3	110	theme	first-degree	626:637	arg1	relatives					639:647	healthy first-degree relatives	618:647	healthy first-degree relatives of patients with Crohn's disease	618:680	METHODS We used the Crohn's and Colitis Canada Genetic Environmental Microbial (CCC-GEM) cohort of healthy first-degree relatives of patients with Crohn's disease.
35850197	1	111	theme	animal	280:285	arg1	studies					287:293	animal studies	280:293	animal studies	280:293	BACKGROUND & AIMS The gut microbiome has been suggested to play a role in gut barrier hemostasis, but data are scarce and limited to animal studies.
35850197	8	112	theme	forest	1046:1051	arg1	algorithm					1064:1072	A random forest classifier algorithm	1037:1072	A random forest classifier algorithm	1037:1072	A random forest classifier algorithm was performed to assess microbial community contribution to barrier function.
35850197	9	113	with	individuals	1369:1379	arg1	≤0.025					1393:1398	an LMR ≤0.025	1386:1398	an LMR ≤0.025	1386:1398	RESULTS Individuals with impaired barrier function (LMR >0.025) had reduced alpha-diversity (Chao1 index, P = 4.0e-4) and altered beta-diversity (Bray-Curtis dissimilarity index, R2 = 0.001, P = 1.0e-3) compared with individuals with an LMR ≤0.025.
35850197	11	114	theme	tryptophan	1742:1751	arg1	biosynthesis					1715:1726	decreased biosynthesis	1705:1726	decreased biosynthesis of glutamate, tryptophan, and threonine	1705:1766	Four genera (decreased prevalence of Adlercreutzia, Clostridia UCG 014, and Clostridium sensu stricto 1 and increased abundance of Colidextribacter) and 8 pathways (including decreased biosynthesis of glutamate, tryptophan, and threonine) were replicated in the validation cohort.
35850197	13	115	theme	barrier	2047:2053	arg1	function					2055:2062	gut barrier function	2043:2062	gut barrier function	2043:2062	CONCLUSIONS The gut microbiome community and pathways are associated with changes in gut barrier function.
35850197	4	116	theme	lactulose-to-mannitol	759:779	arg1	LMR					788:790	LMR	788:790	LMR	788:790	Gut barrier function was assessed using the urinary fractional excretion of lactulose-to-mannitol ratio (LMR).
35850197	4	116	theme	lactulose-to-mannitol	759:779	arg1	ratio					781:785	lactulose-to-mannitol ratio	759:785	lactulose-to-mannitol ratio (LMR)	759:791	Gut barrier function was assessed using the urinary fractional excretion of lactulose-to-mannitol ratio (LMR).
37241738	3	0	from	metabolism	689:698	arg1	mice					721:724	HFD-induced obese mice	703:724	HFD-induced obese mice	703:724	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	5	1	theme	key	1177:1179	arg1	genera					1191:1196	the key bacterial genera	1173:1196	the key bacterial genera	1173:1196	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	5	1	theme	key	1177:1179	arg1	Muribaculum					1152:1162	Muribaculum	1152:1162	Muribaculum	1152:1162	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	5	1	theme	key	1177:1179	arg1	Bifidobacterium					1132:1146	Bifidobacterium	1132:1146	Bifidobacterium	1132:1146	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	1	2	theme	obesity-related	169:183	arg1	disorders					185:193	obesity-related disorders	169:193	obesity-related disorders	169:193	The role of inulin in alleviating obesity-related disorders has been documented; yet, its underlying mechanisms still need to be further investigated.
37241738	0	3	theme	Microbiota	84:93	arg1	Transplantation					95:109	Fecal Microbiota Transplantation	78:109	Fecal Microbiota Transplantation from Inulin-Dosed Mice	78:132	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	3	4	theme	HFD-induced	703:713	arg1	mice					721:724	HFD-induced obese mice	703:724	HFD-induced obese mice	703:724	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	2	5	from	link	334:337	arg1	disorders					421:429	obesity-related disorders	405:429	obesity-related disorders	405:429	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	0	6	theme	Inulin-Dosed	116:127	arg1	Mice					129:132	Inulin-Dosed Mice	116:132	Inulin-Dosed Mice	116:132	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	0	7	from	Amelioration	0:11	arg1	Mice					63:66	High-Fat Diet-Fed Mice	45:66	High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice	45:132	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	6	8	theme	obesity-related	1254:1268	arg1	disorders					1270:1278	obesity-related disorders	1254:1278	obesity-related disorders	1254:1278	Therefore, our results suggest that inulin ameliorates obesity-related disorders by targeting the gut microbiota.
37241738	4	9	theme	Bifidobacterium	897:911	arg1	abundances					883:892	the relative abundances	870:892	the relative abundances of Bifidobacterium and Muribaculum	870:927	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	2	10	theme	recipient	529:537	arg1	mice					539:542	high-fat diet (HFD)-induced obese recipient mice	495:542	high-fat diet (HFD)-induced obese recipient mice	495:542	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	4	11	theme	microbiota	799:808	arg1	composition					776:786	composition	776:786	composition	776:786	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	11	theme	microbiota	799:808	arg1	structure					762:770	structure	762:770	structure	762:770	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	2	12	theme	obesity-related	405:419	arg1	disorders					421:429	obesity-related disorders	405:429	obesity-related disorders	405:429	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	2	13	theme	obese	523:527	arg1	mice					539:542	high-fat diet (HFD)-induced obese recipient mice	495:542	high-fat diet (HFD)-induced obese recipient mice	495:542	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	4	14	from	composition	776:786	arg1	mice					831:834	HFD-induced obese mice	813:834	HFD-induced obese mice	813:834	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	15	theme	gut	795:797	arg1	microbiota					799:808	the gut microbiota	791:808	the gut microbiota	791:808	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	2	16	theme	inulin-dosed	474:485	arg1	mice					487:490	inulin-dosed mice	474:490	inulin-dosed mice	474:490	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	4	17	from	increases	857:865	arg1	Lachnoclostridium					979:995	Lachnoclostridium	979:995	Lachnoclostridium	979:995	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	17	from	increases	857:865	arg1	abundances					883:892	the relative abundances	870:892	the relative abundances of Bifidobacterium and Muribaculum	870:927	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	17	from	increases	857:865	arg1	unidentified_Lachnospiraceae					946:973	unidentified_Lachnospiraceae	946:973	unidentified_Lachnospiraceae	946:973	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	18	theme	relative	874:881	arg1	abundances					883:892	the relative abundances	870:892	the relative abundances of Bifidobacterium and Muribaculum	870:927	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	0	19	theme	Fecal	78:82	arg1	Transplantation					95:109	Fecal Microbiota Transplantation	78:109	Fecal Microbiota Transplantation from Inulin-Dosed Mice	78:132	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	2	20	theme	inulin	395:400	arg1	effect					385:390	the beneficial effect	370:390	the beneficial effect of inulin on obesity-related disorders	370:429	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	2	20	theme	inulin	395:400	arg1	link					334:337	the causative link	320:337	the causative link between the gut microbiota	320:364	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	2	21	from	effect	385:390	arg1	disorders					421:429	obesity-related disorders	405:429	obesity-related disorders	405:429	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	2	22	theme	causative	324:332	arg1	link					334:337	the causative link	320:337	the causative link between the gut microbiota	320:364	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	0	23	theme	Disorders	32:40	arg1	Amelioration					0:11	Amelioration	0:11	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.	0:133	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	4	24	from	structure	762:770	arg1	mice					831:834	HFD-induced obese mice	813:834	HFD-induced obese mice	813:834	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	5	25	theme	inulin	1052:1057	arg1	effects					1041:1047	these favorable effects	1025:1047	these favorable effects of inulin	1025:1057	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	6	26	theme	gut	1297:1299	arg1	microbiota					1301:1310	the gut microbiota	1293:1310	the gut microbiota	1293:1310	Therefore, our results suggest that inulin ameliorates obesity-related disorders by targeting the gut microbiota.
37241738	3	27	theme	inulin	567:572	arg1	supplementation					574:588	inulin supplementation	567:588	inulin supplementation	567:588	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	2	28	theme	fecal	452:456	arg1	microbiota					458:467	the fecal microbiota	448:467	the fecal microbiota	448:467	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	4	29	with	Treatment	727:735	arg1	inulin					742:747	inulin	742:747	inulin	742:747	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	0	30	theme	High-Fat	45:52	arg1	Mice					63:66	High-Fat Diet-Fed Mice	45:66	High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice	45:132	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	1	31	dep	documented	204:213	arg1	need					253:256	need	253:256	need to be further investigated	253:283	The role of inulin in alleviating obesity-related disorders has been documented; yet, its underlying mechanisms still need to be further investigated.
37241738	2	32	theme	high-fat	495:502	arg1	HFD					510:512	HFD	510:512	HFD	510:512	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	2	32	theme	high-fat	495:502	arg1	diet					504:507	high-fat diet	495:507	high-fat diet (HFD)	495:513	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	3	33	theme	fat	616:618	arg1	accumulation					620:631	fat accumulation	616:631	fat accumulation	616:631	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	1	34	theme	underlying	225:234	arg1	mechanisms					236:245	its underlying mechanisms	221:245	its underlying mechanisms	221:245	The role of inulin in alleviating obesity-related disorders has been documented; yet, its underlying mechanisms still need to be further investigated.
37241738	5	35	theme	microbiota	1100:1109	arg1	transplantation					1111:1125	fecal microbiota transplantation	1094:1125	fecal microbiota transplantation	1094:1125	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	3	36	theme	body	603:606	arg1	weight					608:613	body weight	603:613	body weight	603:613	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	0	37	theme	Diet-Fed	54:61	arg1	Mice					63:66	High-Fat Diet-Fed Mice	45:66	High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice	45:132	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	4	38	theme	Muribaculum	917:927	arg1	abundances					883:892	the relative abundances	870:892	the relative abundances of Bifidobacterium and Muribaculum	870:927	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	5	39	theme	fecal	1094:1098	arg1	transplantation					1111:1125	fecal microbiota transplantation	1094:1125	fecal microbiota transplantation	1094:1125	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	4	40	from	decreases	933:941	arg1	Lachnoclostridium					979:995	Lachnoclostridium	979:995	Lachnoclostridium	979:995	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	40	from	decreases	933:941	arg1	abundances					883:892	the relative abundances	870:892	the relative abundances of Bifidobacterium and Muribaculum	870:927	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	40	from	decreases	933:941	arg1	unidentified_Lachnospiraceae					946:973	unidentified_Lachnospiraceae	946:973	unidentified_Lachnospiraceae	946:973	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	2	41	theme	gut	351:353	arg1	microbiota					355:364	the gut microbiota	347:364	the gut microbiota	347:364	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	4	42	theme	obese	825:829	arg1	mice					831:834	HFD-induced obese mice	813:834	HFD-induced obese mice	813:834	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	0	43	from	Mice	129:132	arg1	Transplantation					95:109	Fecal Microbiota Transplantation	78:109	Fecal Microbiota Transplantation from Inulin-Dosed Mice	78:132	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	4	44	theme	HFD-induced	813:823	arg1	mice					831:834	HFD-induced obese mice	813:834	HFD-induced obese mice	813:834	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	4	45	dep	structure	762:770	arg1	the					758:760	the	758:760	the	758:760	Treatment with inulin reshaped the structure and composition of the gut microbiota in HFD-induced obese mice, as characterized by increases in the relative abundances of Bifidobacterium and Muribaculum and decreases in unidentified_Lachnospiraceae and Lachnoclostridium.
37241738	5	46	theme	favorable	1031:1039	arg1	effects					1041:1047	these favorable effects	1025:1047	these favorable effects of inulin	1025:1057	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	3	47	theme	systemic	638:645	arg1	inflammation					647:658	systemic inflammation	638:658	systemic inflammation	638:658	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	1	48	theme	inulin	147:152	arg1	role					139:142	The role	135:142	The role of inulin in alleviating obesity-related disorders	135:193	The role of inulin in alleviating obesity-related disorders has been documented; yet, its underlying mechanisms still need to be further investigated.
37241738	2	49	theme	-induced	514:521	arg1	mice					539:542	high-fat diet (HFD)-induced obese recipient mice	495:542	high-fat diet (HFD)-induced obese recipient mice	495:542	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37241738	3	50	theme	glucose	681:687	arg1	metabolism					689:698	glucose metabolism	681:698	glucose metabolism in HFD-induced obese mice	681:724	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	3	51	theme	obese	715:719	arg1	mice					721:724	HFD-induced obese mice	703:724	HFD-induced obese mice	703:724	The results show that inulin supplementation can decrease body weight, fat accumulation, and systemic inflammation and can also enhance glucose metabolism in HFD-induced obese mice.
37241738	5	52	theme	bacterial	1181:1189	arg1	genera					1191:1196	the key bacterial genera	1173:1196	the key bacterial genera	1173:1196	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	5	52	theme	bacterial	1181:1189	arg1	Muribaculum					1152:1162	Muribaculum	1152:1162	Muribaculum	1152:1162	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	5	52	theme	bacterial	1181:1189	arg1	Bifidobacterium					1132:1146	Bifidobacterium	1132:1146	Bifidobacterium	1132:1146	In addition, we found that these favorable effects of inulin could be partially transferable by fecal microbiota transplantation, and Bifidobacterium and Muribaculum might be the key bacterial genera.
37241738	0	53	theme	Obesity-Related	16:30	arg1	Disorders					32:40	Obesity-Related Disorders	16:40	Obesity-Related Disorders	16:40	Amelioration of Obesity-Related Disorders in High-Fat Diet-Fed Mice following Fecal Microbiota Transplantation from Inulin-Dosed Mice.
37241738	2	54	theme	beneficial	374:383	arg1	effect					385:390	the beneficial effect	370:390	the beneficial effect of inulin on obesity-related disorders	370:429	This study attempted to elucidate the causative link between the gut microbiota and the beneficial effect of inulin on obesity-related disorders via transferring the fecal microbiota from inulin-dosed mice to high-fat diet (HFD)-induced obese recipient mice.
37379943	7	0	theme	antioxidant	1368:1378	arg1	activity					1380:1387	in vitro antioxidant activity	1359:1387	in vitro antioxidant activity	1359:1387	Further CS-Ag nanocomposite was subjected for in vitro antioxidant activity.
37379943	4	1	theme	A549	781:784	arg1	line					792:795	Lung cancer (A549) cell line	768:795	Lung cancer (A549) cell line	768:795	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	3	2	theme	NaBH4	643:647	arg1	presence					631:638	the presence	627:638	the presence of NaBH4	627:647	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	6	3	dep	drug	1283:1286	arg1	acid					1297:1300	ascorbic acid	1288:1300	the standard drug ascorbic acid (99.27 %)	1270:1310	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	6	3	dep	drug	1283:1286	arg1	%					1309:1309	99.27 %	1303:1309	99.27 %	1303:1309	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	0	4	theme	nitroarenes	99:109	arg1	reduction					86:94	reduction	86:94	reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications	86:173	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	7	5	theme	in	1359:1360	arg1	activity					1380:1387	in vitro antioxidant activity	1359:1387	in vitro antioxidant activity	1359:1387	Further CS-Ag nanocomposite was subjected for in vitro antioxidant activity.
37379943	4	6	theme	cell	787:790	arg1	line					792:795	Lung cancer (A549) cell line	768:795	Lung cancer (A549) cell line	768:795	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	5	7	theme	CS-Ag	924:928	arg1	NC					930:931	The CS-Ag NC	920:931	The CS-Ag NC	920:931	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	7	8	theme	Further	1313:1319	arg1	nanocomposite					1327:1339	Further CS-Ag nanocomposite	1313:1339	Further CS-Ag nanocomposite	1313:1339	Further CS-Ag nanocomposite was subjected for in vitro antioxidant activity.
37379943	1	9	theme	kiwi	276:279	arg1	juice					287:291	kiwi fruit juice	276:291	kiwi fruit juice	276:291	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	0	10	theme	anti-proliferative	112:129	arg1	reduction					86:94	reduction	86:94	reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications	86:173	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	6	11	theme	CS-Ag	1176:1180	arg1	NC					1182:1183	CS-Ag NC	1176:1183	CS-Ag NC	1176:1183	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	3	12	theme	CS-Ag	506:510	arg1	reductant					652:660	reductant	652:660	reductant	652:660	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	3	12	theme	CS-Ag	506:510	arg1	catalyst					550:557	catalyst	550:557	catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4	550:647	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	3	12	theme	CS-Ag	506:510	arg1	nanocomposite					512:524	The prepared CS-Ag nanocomposite	493:524	The prepared CS-Ag nanocomposite	493:524	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	2	13	theme	characterization	389:404	arg1	SEM-EDX					430:436	SEM-EDX	430:436	SEM-EDX	430:436	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	13	theme	characterization	389:404	arg1	size					467:470	particle size	458:470	particle size	458:470	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	13	theme	characterization	389:404	arg1	UV-visible					439:448	UV-visible	439:448	UV-visible	439:448	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	13	theme	characterization	389:404	arg1	techniques					406:415	characterization techniques	389:415	characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential	389:490	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	13	theme	characterization	389:404	arg1	XRD					425:427	XRD	425:427	XRD	425:427	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	13	theme	characterization	389:404	arg1	FT-IR					451:455	FT-IR	451:455	FT-IR	451:455	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	13	theme	characterization	389:404	arg1	potential					482:490	zeta potential	477:490	zeta potential	477:490	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	7	14	theme	CS-Ag	1321:1325	arg1	nanocomposite					1327:1339	Further CS-Ag nanocomposite	1313:1339	Further CS-Ag nanocomposite	1313:1339	Further CS-Ag nanocomposite was subjected for in vitro antioxidant activity.
37379943	0	15	theme	chitosan‑silver	35:49	arg1	synthesis					22:30	Novel jointured green synthesis	0:30	Novel jointured green synthesis of chitosan‑silver	0:49	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	4	16	theme	CS-Ag	718:722	arg1	NC					724:725	CS-Ag NC	718:725	CS-Ag NC	718:725	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	4	17	theme	respective	842:851	arg1	IC50values					853:862	their respective IC50values	836:862	their respective IC50values observed	836:871	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	0	18	theme	jointured	6:14	arg1	synthesis					22:30	Novel jointured green synthesis	0:30	Novel jointured green synthesis of chitosan‑silver	0:49	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	3	19	theme	aqueous	666:672	arg1	medium					674:679	aqueous medium	666:679	aqueous medium at room temperature	666:699	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	4	20	theme	cancer	773:778	arg1	line					792:795	Lung cancer (A549) cell line	768:795	Lung cancer (A549) cell line	768:795	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	6	21	theme	closure	1213:1219	arg1	identical					1249:1257	identical	1249:1257	identical	1249:1257	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	6	21	theme	closure	1213:1219	arg1	percentage					1193:1202	the percentage	1189:1202	the percentage of wound closure (97.92 %)	1189:1229	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	0	22	theme	Novel	0:4	arg1	synthesis					22:30	Novel jointured green synthesis	0:30	Novel jointured green synthesis of chitosan‑silver	0:49	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	1	23	theme	fruit	281:285	arg1	juice					287:291	kiwi fruit juice	276:291	kiwi fruit juice	276:291	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	4	24	theme	Lung	768:771	arg1	line					792:795	Lung cancer (A549) cell line	768:795	Lung cancer (A549) cell line	768:795	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	1	25	theme	simple	196:201	arg1	synthesis					209:217	the simple green synthesis	192:217	the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC)	192:261	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	7	26	dep	in	1359:1360	arg1	vitro					1362:1366	vitro	1362:1366	vitro	1362:1366	Further CS-Ag nanocomposite was subjected for in vitro antioxidant activity.
37379943	0	27	theme	wound	132:136	arg1	healing					138:144	wound healing	132:144	wound healing	132:144	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	3	28	from	reduction	566:574	arg1	presence					631:638	the presence	627:638	the presence of NaBH4	627:647	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	1	29	theme	green	203:207	arg1	synthesis					209:217	the simple green synthesis	192:217	the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC)	192:261	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	0	30	theme	green	16:20	arg1	synthesis					22:30	Novel jointured green synthesis	0:30	Novel jointured green synthesis of chitosan‑silver	0:49	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	3	31	theme	room	684:687	arg1	temperature					689:699	room temperature	684:699	room temperature	684:699	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	4	32	theme	NC	724:725	arg1	toxicity					706:713	The toxicity	702:713	The toxicity of CS-Ag NC	702:725	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	3	33	used	used	542:545	arg2	catalyst					550:557	catalyst	550:557	catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4	550:647	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	3	33	used	used	542:545	arg2	nanocomposite					512:524	The prepared CS-Ag nanocomposite	493:524	The prepared CS-Ag nanocomposite	493:524	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	3	33	used	used	542:545	arg2	reductant					652:660	reductant	652:660	reductant	652:660	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	2	34	theme	particle	458:465	arg1	size					467:470	particle size	458:470	particle size	458:470	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	1	35	theme	reducing	296:303	arg1	agent					305:309	reducing agent	296:309	reducing agent	296:309	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	4	36	theme	cell	822:825	arg1	line					827:830	Oral cancer (KB-3-1) cell line	801:830	Oral cancer (KB-3-1) cell line	801:830	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	4	37	theme	L929	751:754	arg1	line					762:765	Normal (L929) cell line	743:765	Normal (L929) cell line	743:765	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	5	38	theme	cell	1041:1044	arg1	lines					1046:1050	cell lines	1041:1050	cell lines	1041:1050	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	0	39	theme	healing	138:144	arg1	reduction					86:94	reduction	86:94	reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications	86:173	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	5	40	theme	cell	982:985	arg1	percentage					997:1006	the cell viability percentage	978:1006	the cell viability percentage for normal, lung and oral cancer cell lines	978:1050	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	5	40	theme	cell	982:985	arg1	42.87 ± 0.0060					1069:1082	42.87 ± 0.0060	1069:1082	42.87 ± 0.0060	1069:1082	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	3	41	theme	4-nitrophenol	579:591	arg1	reduction					566:574	the reduction	562:574	the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4	562:647	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	1	42	theme	chitosan‑silver	222:236	arg1	nanocomposite					238:250	chitosan‑silver nanocomposite	222:250	chitosan‑silver nanocomposite (CS-Ag NC)	222:261	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	1	42	theme	chitosan‑silver	222:236	arg1	NC					259:260	CS-Ag NC	253:260	CS-Ag NC	253:260	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	5	43	dep	normal	1012:1017	arg1	lines					1046:1050	cell lines	1041:1050	cell lines	1041:1050	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	0	44	theme	applications	162:173	arg1	reduction					86:94	reduction	86:94	reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications	86:173	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	5	45	theme	viability	987:995	arg1	percentage					997:1006	the cell viability percentage	978:1006	the cell viability percentage for normal, lung and oral cancer cell lines	978:1050	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	5	45	theme	viability	987:995	arg1	42.87 ± 0.0060					1069:1082	42.87 ± 0.0060	1069:1082	42.87 ± 0.0060	1069:1082	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	4	46	theme	cell	757:760	arg1	line					762:765	Normal (L929) cell line	743:765	Normal (L929) cell line	743:765	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	6	47	theme	wound	1207:1211	arg1	closure					1213:1219	wound closure	1207:1219	wound closure (97.92 %)	1207:1229	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	6	47	theme	wound	1207:1211	arg1	%					1228:1228	97.92 %	1222:1228	97.92 %	1222:1228	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	5	48	theme	oral	1029:1032	arg1	cancer					1034:1039	oral cancer	1029:1039	oral cancer	1029:1039	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	6	49	theme	ascorbic	1288:1295	arg1	acid					1297:1300	ascorbic acid	1288:1300	the standard drug ascorbic acid (99.27 %)	1270:1310	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	6	49	theme	ascorbic	1288:1295	arg1	%					1309:1309	99.27 %	1303:1309	99.27 %	1303:1309	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	0	50	theme	antioxidant	150:160	arg1	applications					162:173	antioxidant applications	150:173	antioxidant applications	150:173	Novel jointured green synthesis of chitosan‑silver nanocomposite: An approach towards reduction of nitroarenes, anti-proliferative, wound healing and antioxidant applications.
37379943	3	51	from	temperature	689:699	arg1	medium					674:679	aqueous medium	666:679	aqueous medium at room temperature	666:699	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	4	52	theme	cancer	806:811	arg1	line					827:830	Oral cancer (KB-3-1) cell line	801:830	Oral cancer (KB-3-1) cell line	801:830	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	5	53	theme	significant	943:953	arg1	activity					965:972	significant cytotoxic activity	943:972	significant cytotoxic activity	943:972	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	6	54	theme	cell	1142:1145	arg1	migration					1147:1155	Stronger cell migration	1133:1155	Stronger cell migration	1133:1155	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	1	55	theme	nanocomposite	238:250	arg1	synthesis					209:217	the simple green synthesis	192:217	the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC)	192:261	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	4	56	theme	Oral	801:804	arg1	KB-3-1					814:819	KB-3-1	814:819	KB-3-1	814:819	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	4	56	theme	Oral	801:804	arg1	cancer					806:811	Oral cancer	801:811	Oral cancer (KB-3-1) cell line	801:830	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	5	57	theme	cytotoxic	955:963	arg1	activity					965:972	significant cytotoxic activity	943:972	significant cytotoxic activity	943:972	The CS-Ag NC displayed significant cytotoxic activity and the cell viability percentage for normal, lung and oral cancer cell lines were found to be 42.87 ± 0.0060, 31.28 ± 0.0045 and 35.90 ± 0.0065 respectively.
37379943	3	58	theme	prepared	497:504	arg1	reductant					652:660	reductant	652:660	reductant	652:660	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	3	58	theme	prepared	497:504	arg1	catalyst					550:557	catalyst	550:557	catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4	550:647	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	3	58	theme	prepared	497:504	arg1	nanocomposite					512:524	The prepared CS-Ag nanocomposite	493:524	The prepared CS-Ag nanocomposite	493:524	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	3	59	from	catalyst	550:557	arg1	reduction					566:574	the reduction	562:574	the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4	562:647	The prepared CS-Ag nanocomposite was effectively used as catalyst in the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) in the presence of NaBH4 as reductant, in aqueous medium at room temperature.
37379943	2	60	theme	zeta	477:480	arg1	potential					482:490	zeta potential	477:490	zeta potential	477:490	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	4	61	theme	Normal	743:748	arg1	line					762:765	Normal (L929) cell line	743:765	Normal (L929) cell line	743:765	The toxicity of CS-Ag NC was assessed on Normal (L929) cell line, Lung cancer (A549) cell line and Oral cancer (KB-3-1) cell line and their respective IC50values observed were 83.52 μg/mL, 66.74 μg/mL and 75.11 μg/mL.
37379943	6	62	theme	Stronger	1133:1140	arg1	migration					1147:1155	Stronger cell migration	1133:1155	Stronger cell migration	1133:1155	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	2	63	theme	NC	364:365	arg1	morphology					327:336	morphology	327:336	morphology	327:336	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	63	theme	NC	364:365	arg1	composition					343:353	composition	343:353	composition	343:353	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	2	63	theme	NC	364:365	arg1	structure					316:324	The structure	312:324	The structure	312:324	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
37379943	1	64	theme	CS-Ag	253:257	arg1	nanocomposite					238:250	chitosan‑silver nanocomposite	222:250	chitosan‑silver nanocomposite (CS-Ag NC)	222:261	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	1	64	theme	CS-Ag	253:257	arg1	NC					259:260	CS-Ag NC	253:260	CS-Ag NC	253:260	Here we present the simple green synthesis of chitosan‑silver nanocomposite (CS-Ag NC) by employing kiwi fruit juice as reducing agent.
37379943	6	65	theme	standard	1274:1281	arg1	drug					1283:1286	the standard drug ascorbic acid (99.27 %)	1270:1310	the standard drug ascorbic acid (99.27 %)	1270:1310	Stronger cell migration was exemplified by CS-Ag NC and the percentage of wound closure (97.92 %) was substantially identical to that of the standard drug ascorbic acid (99.27 %).
37379943	2	66	theme	CS-Ag	358:362	arg1	NC					364:365	CS-Ag NC	358:365	CS-Ag NC	358:365	The structure, morphology, and composition of CS-Ag NC were determined using characterization techniques such as XRD, SEM-EDX, UV-visible, FT-IR, particle size, and zeta potential.
35899535	12	0	theme	Further	1712:1718	arg1	studies					1720:1726	Further studies	1712:1726	Further studies	1712:1726	Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.
35899535	6	1	theme	dietary	996:1002	arg1	intake					1010:1015	Daily dietary fiber intake	990:1015	Daily dietary fiber intake in the SMID group	990:1033	Daily dietary fiber intake in the SMID group was evaluated using questionnaires.
35899535	4	2	dep	group	754:758	arg1	age					768:770	median age	761:770	median age	761:770	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	2	dep	group	754:758	arg1	years					778:782	10.0 years	773:782	10.0 years	773:782	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	11	3	with	Children	1563:1570	arg1	SMIDs					1577:1581	SMIDs	1577:1581	SMIDs	1577:1581	CONCLUSION Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake.
35899535	9	4	theme	butyric	1309:1315	arg1	bacteria					1332:1339	butyric acid-producing bacteria	1309:1339	butyric acid-producing bacteria	1309:1339	Compared with the HC group, Clostridiales and butyric acid-producing bacteria were less abundant and Bacteroidales were more abundant in the SMID group.
35899535	4	5	theme	children	801:808	arg1	samples					640:646	fecal samples	634:646	fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years)	634:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	9	6	from	group	1409:1413	arg1	abundant					1388:1395	abundant	1388:1395	abundant	1388:1395	Compared with the HC group, Clostridiales and butyric acid-producing bacteria were less abundant and Bacteroidales were more abundant in the SMID group.
35899535	10	7	theme	average	1501:1507	arg1	requirement					1509:1519	the estimated average requirement	1487:1519	the estimated average requirement for healthy Japanese children	1487:1549	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	3	8	theme	gut	542:544	arg1	microbiota					546:555	the gut microbiota	538:555	the gut microbiota	538:555	Therefore, we aimed to examine the characteristics of the gut microbiota in children with SMIDs.
35899535	1	9	theme	intellectual	178:189	arg1	disabilities					191:202	severe motor and intellectual disabilities	161:202	disabilities	191:202	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	4	10	theme	gene	598:601	arg1	sequencing					603:612	rRNA gene sequencing	593:612	rRNA gene sequencing	593:612	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	11	theme	median	839:844	arg1	age					846:848	median age	839:848	median age	839:848	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	10	12	theme	Japanese	1533:1540	arg1	children					1542:1549	healthy Japanese children	1525:1549	healthy Japanese children	1525:1549	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	0	13	theme	pilot	123:127	arg1	study					129:133	A pilot study	121:133	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.	0:134	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	1	14	theme	gut	378:380	arg1	microbiota					382:391	the gut microbiota	374:391	the gut microbiota	374:391	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	0	15	theme	gut	17:19	arg1	microbiota					21:30	the gut microbiota	13:30	the gut microbiota	13:30	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	4	16	theme	healthy	811:817	arg1	group					832:836	healthy control [HC] group	811:836	healthy control [HC] group	811:836	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	16	theme	healthy	811:817	arg1	children					801:808	19 healthy children	790:808	19 healthy children (healthy control [HC] group: median age, 9.0 years)	790:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	5	17	theme	acid-producing	932:945	arg1	bacteria					947:954	butyric acid-producing bacteria	924:954	butyric acid-producing bacteria	924:954	Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups.
35899535	4	18	with	children	801:808	arg1	SMIDs					668:672	SMIDs	668:672	SMIDs	668:672	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	19	theme	[HC	827:829	arg1	group					832:836	healthy control [HC] group	811:836	healthy control [HC] group	811:836	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	19	theme	[HC	827:829	arg1	children					801:808	19 healthy children	790:808	19 healthy children (healthy control [HC] group: median age, 9.0 years)	790:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	0	20	theme	severe	49:54	arg1	motor					56:60	severe motor and intellectual disabilities	49:90	motor	56:60	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	2	21	with	children	451:458	arg1	SMIDs					465:469	SMIDs	465:469	SMIDs	465:469	However, studies on the composition of gut microbiota in children with SMIDs are limited.
35899535	1	22	theme	BACKGROUND	136:145	arg1	Children					147:154	BACKGROUND Children	136:154	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs)	136:210	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	12	23	from	dysbiosis	1819:1827	arg1	children					1832:1839	children	1832:1839	children with SMIDs	1832:1850	Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.
35899535	8	24	theme	diversity	1213:1221	arg1	analysis					1223:1230	Beta diversity analysis	1208:1230	Beta diversity analysis	1208:1230	Beta diversity analysis identified different clusters.
35899535	11	25	theme	dietary	1690:1696	arg1	intake					1704:1709	their low dietary fiber intake	1680:1709	their low dietary fiber intake	1680:1709	CONCLUSION Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake.
35899535	7	26	theme	diversity	1118:1126	arg1	indices					1103:1109	The Shannon and Simpson indices	1079:1109	RESULTS The Shannon and Simpson indices (alpha diversity indices)	1071:1135	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	7	26	theme	diversity	1118:1126	arg1	indices					1128:1134	alpha diversity indices	1112:1134	alpha diversity indices	1112:1134	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	11	27	from	alteration	1605:1614	arg1	diversity					1633:1641	the microbial diversity	1619:1641	the microbial diversity	1619:1641	CONCLUSION Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake.
35899535	2	28	from	composition	418:428	arg1	children					451:458	children	451:458	children with SMIDs	451:469	However, studies on the composition of gut microbiota in children with SMIDs are limited.
35899535	4	29	theme	gastric	735:741	arg1	tube					743:746	gastric tube	735:746	gastric tube	735:746	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	0	30	theme	enteral	102:108	arg1	nutrition					110:118	enteral nutrition	102:118	enteral nutrition	102:118	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	7	31	theme	Shannon	1083:1089	arg1	indices					1103:1109	The Shannon and Simpson indices	1079:1109	RESULTS The Shannon and Simpson indices (alpha diversity indices)	1071:1135	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	7	31	theme	Shannon	1083:1089	arg1	indices					1128:1134	alpha diversity indices	1112:1134	alpha diversity indices	1112:1134	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	1	32	theme	adequate	291:298	arg1	exercise					300:307	adequate exercise	291:307	adequate exercise	291:307	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	7	33	theme	HC	1198:1199	arg1	group					1201:1205	the HC group	1194:1205	the HC group	1194:1205	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	11	34	theme	microbial	1623:1631	arg1	diversity					1633:1641	the microbial diversity	1619:1641	the microbial diversity	1619:1641	CONCLUSION Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake.
35899535	4	35	theme	median	761:766	arg1	age					768:770	median age	761:770	median age	761:770	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	0	36	theme	microbiota	21:30	arg1	Dysbiosis					0:8	Dysbiosis	0:8	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.	0:134	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	12	37	theme	synbiotics	1782:1791	arg1	intervention					1738:1749	the intervention	1734:1749	the intervention of prebiotics, probiotics, and synbiotics	1734:1791	Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.
35899535	2	38	theme	microbiota	437:446	arg1	composition					418:428	the composition	414:428	the composition of gut microbiota in children with SMIDs	414:469	However, studies on the composition of gut microbiota in children with SMIDs are limited.
35899535	5	39	theme	butyric	924:930	arg1	bacteria					947:954	butyric acid-producing bacteria	924:954	butyric acid-producing bacteria	924:954	Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups.
35899535	6	40	theme	SMID	1024:1027	arg1	group					1029:1033	the SMID group	1020:1033	the SMID group	1020:1033	Daily dietary fiber intake in the SMID group was evaluated using questionnaires.
35899535	10	41	theme	Dietary	1416:1422	arg1	intake					1430:1435	Dietary fiber intake	1416:1435	Dietary fiber intake in the SMID group	1416:1453	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	9	42	theme	HC	1281:1282	arg1	group					1284:1288	the HC group	1277:1288	the HC group	1277:1288	Compared with the HC group, Clostridiales and butyric acid-producing bacteria were less abundant and Bacteroidales were more abundant in the SMID group.
35899535	5	43	theme	microbial	884:892	arg1	composition					894:904	microbial composition	884:904	microbial composition	884:904	Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups.
35899535	7	44	theme	SMID	1169:1172	arg1	group					1174:1178	the SMID group	1165:1178	the SMID group	1165:1178	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	0	45	from	Dysbiosis	0:8	arg1	children					35:42	children	35:42	children with severe motor and intellectual disabilities receiving enteral nutrition	35:118	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	0	46	with	children	35:42	arg1	motor					56:60	severe motor and intellectual disabilities	49:90	motor	56:60	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	0	46	with	children	35:42	arg1	disabilities					79:90	severe motor and intellectual disabilities	49:90	disabilities	79:90	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	0	47	dep	Dysbiosis	0:8	arg1	study					129:133	A pilot study	121:133	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.	0:134	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	2	48	from	studies	403:409	arg1	composition					418:428	the composition	414:428	the composition of gut microbiota in children with SMIDs	414:469	However, studies on the composition of gut microbiota in children with SMIDs are limited.
35899535	6	49	theme	fiber	1004:1008	arg1	intake					1010:1015	Daily dietary fiber intake	990:1015	Daily dietary fiber intake in the SMID group	990:1033	Daily dietary fiber intake in the SMID group was evaluated using questionnaires.
35899535	7	50	theme	alpha	1112:1116	arg1	indices					1103:1109	The Shannon and Simpson indices	1079:1109	RESULTS The Shannon and Simpson indices (alpha diversity indices)	1071:1135	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	7	50	theme	alpha	1112:1116	arg1	indices					1128:1134	alpha diversity indices	1112:1134	alpha diversity indices	1112:1134	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	1	51	theme	enteral	248:254	arg1	nutrition					256:264	enteral nutrition	248:264	enteral nutrition	248:264	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	6	52	theme	Daily	990:994	arg1	intake					1010:1015	Daily dietary fiber intake	990:1015	Daily dietary fiber intake in the SMID group	990:1033	Daily dietary fiber intake in the SMID group was evaluated using questionnaires.
35899535	11	53	theme	CONCLUSION	1552:1561	arg1	Children					1563:1570	CONCLUSION Children	1552:1570	CONCLUSION Children with SMIDs	1552:1581	CONCLUSION Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake.
35899535	9	54	theme	acid-producing	1317:1330	arg1	bacteria					1332:1339	butyric acid-producing bacteria	1309:1339	butyric acid-producing bacteria	1309:1339	Compared with the HC group, Clostridiales and butyric acid-producing bacteria were less abundant and Bacteroidales were more abundant in the SMID group.
35899535	4	55	theme	healthy	793:799	arg1	group					832:836	healthy control [HC] group	811:836	healthy control [HC] group	811:836	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	55	theme	healthy	793:799	arg1	children					801:808	19 healthy children	790:808	19 healthy children (healthy control [HC] group: median age, 9.0 years)	790:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	56	with	children	654:661	arg1	SMIDs					668:672	SMIDs	668:672	SMIDs	668:672	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	1	57	theme	severe	161:166	arg1	SMIDs					205:209	SMIDs	205:209	SMIDs	205:209	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	1	57	theme	severe	161:166	arg1	motor					168:172	severe motor and intellectual disabilities	161:202	motor	168:172	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	10	58	theme	requirement	1509:1519	arg1	requirement					1509:1519	the estimated average requirement	1487:1519	the estimated average requirement for healthy Japanese children	1487:1549	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	10	58	theme	requirement	1509:1519	arg1	two-thirds					1473:1482	two-thirds	1473:1482	two-thirds	1473:1482	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	4	59	theme	fecal	634:638	arg1	samples					640:646	fecal samples	634:646	fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years)	634:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	10	60	theme	estimated	1491:1499	arg1	requirement					1509:1519	the estimated average requirement	1487:1519	the estimated average requirement for healthy Japanese children	1487:1549	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	12	61	with	studies	1720:1726	arg1	intervention					1738:1749	the intervention	1734:1749	the intervention of prebiotics, probiotics, and synbiotics	1734:1791	Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.
35899535	8	62	theme	Beta	1208:1211	arg1	analysis					1223:1230	Beta diversity analysis	1208:1230	Beta diversity analysis	1208:1230	Beta diversity analysis identified different clusters.
35899535	4	63	theme	rRNA	593:596	arg1	sequencing					603:612	rRNA gene sequencing	593:612	rRNA gene sequencing	593:612	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	10	64	theme	healthy	1525:1531	arg1	children					1542:1549	healthy Japanese children	1525:1549	healthy Japanese children	1525:1549	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	1	65	theme	microbiota	382:391	arg1	composition					359:369	the composition	355:369	the composition of the gut microbiota	355:391	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	3	66	theme	microbiota	546:555	arg1	characteristics					519:533	the characteristics	515:533	the characteristics of the gut microbiota in children with SMIDs	515:578	Therefore, we aimed to examine the characteristics of the gut microbiota in children with SMIDs.
35899535	1	67	dep	lack	286:289	arg1	lead					320:323	lead	320:323	may lead to dysbiosis	316:336	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	3	68	from	characteristics	519:533	arg1	children					560:567	children	560:567	children with SMIDs	560:578	Therefore, we aimed to examine the characteristics of the gut microbiota in children with SMIDs.
35899535	5	69	theme	bacteria	947:954	arg1	composition					894:904	microbial composition	884:904	microbial composition	884:904	Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups.
35899535	5	69	theme	bacteria	947:954	arg1	abundance					911:919	abundance	911:919	abundance	911:919	Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups.
35899535	5	69	theme	bacteria	947:954	arg1	diversity					873:881	Microbial diversity	863:881	Microbial diversity	863:881	Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups.
35899535	9	70	theme	SMID	1404:1407	arg1	group					1409:1413	the SMID group	1400:1413	the SMID group	1400:1413	Compared with the HC group, Clostridiales and butyric acid-producing bacteria were less abundant and Bacteroidales were more abundant in the SMID group.
35899535	4	71	theme	control	819:825	arg1	group					832:836	healthy control [HC] group	811:836	healthy control [HC] group	811:836	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	71	theme	control	819:825	arg1	children					801:808	19 healthy children	790:808	19 healthy children (healthy control [HC] group: median age, 9.0 years)	790:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	0	72	theme	intellectual	66:77	arg1	disabilities					79:90	severe motor and intellectual disabilities	49:90	disabilities	79:90	Dysbiosis of the gut microbiota in children with severe motor and intellectual disabilities receiving enteral nutrition: A pilot study.
35899535	1	73	with	Children	147:154	arg1	SMIDs					205:209	SMIDs	205:209	SMIDs	205:209	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	1	73	with	Children	147:154	arg1	motor					168:172	severe motor and intellectual disabilities	161:202	motor	168:172	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	1	73	with	Children	147:154	arg1	disabilities					191:202	severe motor and intellectual disabilities	161:202	disabilities	191:202	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	7	74	dep	RESULTS	1071:1077	arg1	indices					1103:1109	The Shannon and Simpson indices	1079:1109	RESULTS The Shannon and Simpson indices (alpha diversity indices)	1071:1135	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	7	74	dep	RESULTS	1071:1077	arg1	indices					1128:1134	alpha diversity indices	1112:1134	alpha diversity indices	1112:1134	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	4	75	theme	children	654:661	arg1	samples					640:646	fecal samples	634:646	fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years)	634:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	11	76	theme	fiber	1698:1702	arg1	intake					1704:1709	their low dietary fiber intake	1680:1709	their low dietary fiber intake	1680:1709	CONCLUSION Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake.
35899535	10	77	from	intake	1430:1435	arg1	group					1449:1453	the SMID group	1440:1453	the SMID group	1440:1453	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	4	78	theme	gastric	716:722	arg1	fistula					724:730	a gastric fistula	714:730	a gastric fistula	714:730	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	79	with	tube	743:746	arg1	SMIDs					668:672	SMIDs	668:672	SMIDs	668:672	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	3	80	with	children	560:567	arg1	SMIDs					574:578	SMIDs	574:578	SMIDs	574:578	Therefore, we aimed to examine the characteristics of the gut microbiota in children with SMIDs.
35899535	5	81	theme	Microbial	863:871	arg1	diversity					873:881	Microbial diversity	863:881	Microbial diversity	863:881	Microbial diversity, microbial composition, and abundance of butyric acid-producing bacteria were compared between the groups.
35899535	11	82	theme	low	1686:1688	arg1	intake					1704:1709	their low dietary fiber intake	1680:1709	their low dietary fiber intake	1680:1709	CONCLUSION Children with SMIDs showed dysbiosis with alteration in the microbial diversity, which could partly be attributed to their low dietary fiber intake.
35899535	8	83	theme	different	1243:1251	arg1	clusters					1253:1260	different clusters	1243:1260	different clusters	1243:1260	Beta diversity analysis identified different clusters.
35899535	4	84	dep	group	832:836	arg1	age					846:848	median age	839:848	median age	839:848	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	84	dep	group	832:836	arg1	years					855:859	9.0 years	851:859	9.0 years	851:859	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	85	theme	enteral	688:694	arg1	nutrition					696:704	enteral nutrition	688:704	enteral nutrition	688:704	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	1	86	from	imbalance	342:350	arg1	composition					359:369	the composition	355:369	the composition of the gut microbiota	355:391	BACKGROUND Children with severe motor and intellectual disabilities (SMIDs) frequently and continuously receive enteral nutrition and medications and lack adequate exercise, which may lead to dysbiosis, an imbalance in the composition of the gut microbiota.
35899535	12	87	with	children	1832:1839	arg1	SMIDs					1846:1850	SMIDs	1846:1850	SMIDs	1846:1850	Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.
35899535	7	88	theme	Simpson	1095:1101	arg1	indices					1103:1109	The Shannon and Simpson indices	1079:1109	RESULTS The Shannon and Simpson indices (alpha diversity indices)	1071:1135	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	7	88	theme	Simpson	1095:1101	arg1	indices					1128:1134	alpha diversity indices	1112:1134	alpha diversity indices	1112:1134	RESULTS The Shannon and Simpson indices (alpha diversity indices) were significantly lower in the SMID group than those in the HC group.
35899535	12	89	theme	prebiotics	1754:1763	arg1	intervention					1738:1749	the intervention	1734:1749	the intervention of prebiotics, probiotics, and synbiotics	1734:1791	Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.
35899535	9	90	from	abundant	1388:1395	arg1	group					1409:1413	the SMID group	1400:1413	the SMID group	1400:1413	Compared with the HC group, Clostridiales and butyric acid-producing bacteria were less abundant and Bacteroidales were more abundant in the SMID group.
35899535	2	91	theme	gut	433:435	arg1	microbiota					437:446	gut microbiota	433:446	gut microbiota	433:446	However, studies on the composition of gut microbiota in children with SMIDs are limited.
35899535	10	92	theme	fiber	1424:1428	arg1	intake					1430:1435	Dietary fiber intake	1416:1435	Dietary fiber intake in the SMID group	1416:1453	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	12	93	theme	probiotics	1766:1775	arg1	intervention					1738:1749	the intervention	1734:1749	the intervention of prebiotics, probiotics, and synbiotics	1734:1791	Further studies, with the intervention of prebiotics, probiotics, and synbiotics, are warranted to improve dysbiosis in children with SMIDs.
35899535	4	94	theme	tube	743:746	arg1	samples					640:646	fecal samples	634:646	fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years)	634:860	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	10	95	theme	SMID	1444:1447	arg1	group					1449:1453	the SMID group	1440:1453	the SMID group	1440:1453	Dietary fiber intake in the SMID group was approximately two-thirds of the estimated average requirement for healthy Japanese children.
35899535	4	96	theme	SMID	749:752	arg1	children					654:661	10 children	651:661	10 children with SMIDs, who received enteral nutrition through a gastric fistula	651:730	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	4	96	theme	SMID	749:752	arg1	group					754:758	SMID group	749:758	SMID group	749:758	METHODS 16S rRNA gene sequencing was performed using fecal samples of 10 children with SMIDs, who received enteral nutrition through a gastric fistula or gastric tube (SMID group: median age, 10.0 years), and 19 healthy children (healthy control [HC] group: median age, 9.0 years).
35899535	6	97	from	intake	1010:1015	arg1	group					1029:1033	the SMID group	1020:1033	the SMID group	1020:1033	Daily dietary fiber intake in the SMID group was evaluated using questionnaires.
35485727	9	0	from	reductions	1263:1272	arg1	occludin					1291:1298	occludin	1291:1298	occludin	1291:1298	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	9	0	from	reductions	1263:1272	arg1	claudin-1					1277:1285	claudin-1	1277:1285	claudin-1	1277:1285	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	1	1	theme	composition	279:289	arg1	dynamics					262:269	associated pathobiological dynamics	235:269	associated pathobiological dynamics of body composition and intestinal barrier function	235:321	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	1	1	theme	composition	279:289	arg1	diets					225:229	nutrient-deficient diets	206:229	nutrient-deficient diets	206:229	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	9	2	from	reduction	1328:1336	arg1	levels					1345:1350	the levels	1341:1350	the levels of claudin-2 and occludin	1341:1376	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	12	3	theme	claudin-1	1609:1617	arg1	transcripts					1632:1642	claudin-1 and occludin transcripts	1609:1642	claudin-1 and occludin transcripts	1609:1642	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	3	4	theme	control	550:556	arg1	diet					558:561	a control diet	548:561	a control diet for 21 d	548:570	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	1	5	theme	intestinal	295:304	arg1	function					314:321	intestinal barrier function	295:321	intestinal barrier function	295:321	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	11	6	theme	physical	1451:1458	arg1	development					1460:1470	physical development	1451:1470	physical development	1451:1470	RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional.
35485727	4	7	theme	quantitative	668:679	arg1	transcription-PCR					689:705	quantitative reverse transcription-PCR	668:705	quantitative reverse transcription-PCR	668:705	The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters.
35485727	0	8	from	composition	110:120	arg1	barrier					96:102	the intestinal morphofunctional barrier	64:102	the intestinal morphofunctional barrier	64:102	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	12	9	from	period	1686:1691	arg1	damage					1556:1561	damage	1556:1561	damage to intestinal permeability	1556:1588	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	12	9	from	period	1686:1691	arg1	levels					1599:1604	reduced levels	1591:1604	reduced levels of claudin-1 and occludin transcripts	1591:1642	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	12	9	from	period	1686:1691	arg1	return					1648:1653	return	1648:1653	return of bowel function in a chronic period	1648:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	2	10	from	development	397:407	arg1	mice					452:455	mice	452:455	mice	452:455	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	4	11	theme	lactulose	626:634	arg1	test					636:639	lactulose test	626:639	lactulose test	626:639	The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters.
35485727	10	12	theme	SGLT-1	1379:1384	arg1	levels					1386:1391	SGLT-1 levels	1379:1391	SGLT-1 levels	1379:1391	SGLT-1 levels decreased on day 21.
35485727	1	13	theme	nutrient-deficient	206:223	arg1	diets					225:229	nutrient-deficient diets	206:229	nutrient-deficient diets	206:229	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	8	14	theme	Lactulose	1136:1144	arg1	ratio					1155:1159	Lactulose:mannitol ratio	1136:1159	Lactulose:mannitol ratio	1136:1159	Lactulose:mannitol ratio increased on day 14 (P < 0·05).
35485727	0	15	theme	physical	135:142	arg1	development					144:154	impaired physical development	126:154	impaired physical development	126:154	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	7	16	theme	crypt	1062:1066	arg1	ratio					1068:1072	the villus:crypt ratio	1051:1072	the villus:crypt ratio on day 7	1051:1081	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	6	17	theme	body	912:915	arg1	water					917:921	total body water	906:921	total body water	906:921	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	12	18	theme	intestinal	1566:1575	arg1	permeability					1577:1588	intestinal permeability	1566:1588	intestinal permeability	1566:1588	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	4	19	theme	intestinal	739:748	arg1	transporters					750:761	intestinal transporters	739:761	intestinal transporters	739:761	The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters.
35485727	0	20	theme	intestinal	68:77	arg1	barrier					96:102	the intestinal morphofunctional barrier	64:102	the intestinal morphofunctional barrier	64:102	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	2	21	from	impact	349:354	arg1	barrier					441:447	intestinal morphofunctional barrier	413:447	intestinal morphofunctional barrier	413:447	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	2	21	from	impact	349:354	arg1	development					397:407	physical development	388:407	physical development	388:407	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	11	22	from	changes	1485:1491	arg1	barrier					1507:1513	intestinal barrier morphofunctional	1496:1530	intestinal barrier morphofunctional	1496:1530	RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional.
35485727	8	23	theme	P	1182:1182	arg1	0·05					1186:1189	P < 0·05	1182:1189	P < 0·05	1182:1189	Lactulose:mannitol ratio increased on day 14 (P < 0·05).
35485727	8	23	theme	P	1182:1182	arg1	day					1174:1176	day 14	1174:1179	day 14 (P < 0·05)	1174:1190	Lactulose:mannitol ratio increased on day 14 (P < 0·05).
35485727	6	24	theme	fat	945:947	arg1	mass					949:952	fat mass	945:952	fat mass	945:952	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	7	25	from	ratio	1068:1072	arg1	day					1077:1079	day 7	1077:1081	day 7	1077:1081	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	12	26	from	damage	1556:1561	arg1	period					1686:1691	a chronic period	1676:1691	a chronic period	1676:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	0	27	theme	body	105:108	arg1	composition					110:120	body composition	105:120	body composition	105:120	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	5	28	theme	P	806:806	arg1	0·05					810:813	P < 0·05	806:813	P < 0·05	806:813	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	28	theme	P	806:806	arg1	weight					798:803	weight	798:803	weight (P < 0·05) from day 5	798:825	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	7	29	from	day	1077:1079	arg1	increase					1039:1046	an increase	1036:1046	an increase	1036:1046	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	3	30	dep	C57BL/6	458:464	arg1	days					470:473	21 days	467:473	21 days of age	467:480	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	2	31	theme	morphofunctional	424:439	arg1	barrier					441:447	intestinal morphofunctional barrier	413:447	intestinal morphofunctional barrier	413:447	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	1	32	theme	barrier	306:312	arg1	function					314:321	intestinal barrier function	295:321	intestinal barrier function	295:321	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	6	33	theme	<	986:986	arg1	0·05					988:991	P < 0·05	984:991	P < 0·05	984:991	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	6	33	theme	<	986:986	arg1	day					977:979	day 7	977:981	day 7 (P < 0·05)	977:992	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	3	34	theme	C57BL/6	458:464	arg1	mice					483:486	C57BL/6 (21 days of age) mice	458:486	C57BL/6 (21 days of age) mice	458:486	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	0	35	theme	diet	33:36	arg1	Consumption					0:10	Consumption	0:10	Consumption of a multi-deficient diet	0:36	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	5	36	theme	<	808:808	arg1	0·05					810:813	P < 0·05	806:813	P < 0·05	806:813	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	36	theme	<	808:808	arg1	weight					798:803	weight	798:803	weight (P < 0·05) from day 5	798:825	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	12	37	theme	transcripts	1632:1642	arg1	damage					1556:1561	damage	1556:1561	damage to intestinal permeability	1556:1588	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	12	37	theme	transcripts	1632:1642	arg1	levels					1599:1604	reduced levels	1591:1604	reduced levels of claudin-1 and occludin transcripts	1591:1642	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	12	37	theme	transcripts	1632:1642	arg1	return					1648:1653	return	1648:1653	return of bowel function in a chronic period	1648:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	2	38	theme	diet	380:383	arg1	impact					349:354	the impact	345:354	the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice	345:455	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	3	39	theme	Brazil	512:517	arg1	diet					534:537	a Northeastern Brazil regional basic diet	497:537	a Northeastern Brazil regional basic diet (RBD)	497:543	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	3	39	theme	Brazil	512:517	arg1	RBD					540:542	RBD	540:542	RBD	540:542	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	0	40	theme	dynamic	45:51	arg1	changes					53:59	dynamic changes	45:59	dynamic changes in the intestinal morphofunctional barrier	45:102	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	7	41	from	increase	1039:1046	arg1	ratio					1068:1072	the villus:crypt ratio	1051:1072	the villus:crypt ratio on day 7	1051:1081	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	7	41	from	increase	1039:1046	arg1	day					1077:1079	day 7	1077:1081	day 7	1077:1081	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	9	42	theme	intestinal	1204:1213	arg1	function					1223:1230	intestinal barrier function	1204:1230	intestinal barrier function on day 14	1204:1240	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	12	43	theme	function	1664:1671	arg1	damage					1556:1561	damage	1556:1561	damage to intestinal permeability	1556:1588	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	12	43	theme	function	1664:1671	arg1	levels					1599:1604	reduced levels	1591:1604	reduced levels of claudin-1 and occludin transcripts	1591:1642	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	12	43	theme	function	1664:1671	arg1	return					1648:1653	return	1648:1653	return of bowel function in a chronic period	1648:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	6	44	theme	RBD	894:896	arg1	intake					884:889	The intake	880:889	The intake of RBD	880:896	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	7	45	theme	P	1125:1125	arg1	0·05					1129:1132	P < 0·05	1125:1132	P < 0·05	1125:1132	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	7	45	theme	P	1125:1125	arg1	days					1109:1112	days 14 and 21	1109:1122	days	1109:1112	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	4	46	theme	bioimpedance	603:614	arg1	analysis					616:623	bioimpedance analysis	603:623	bioimpedance analysis	603:623	The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters.
35485727	2	47	theme	physical	388:395	arg1	development					397:407	physical development	388:407	physical development	388:407	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	12	48	theme	bowel	1658:1662	arg1	function					1664:1671	bowel function	1658:1671	bowel function in a chronic period	1658:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	1	49	theme	body	274:277	arg1	composition					279:289	body composition	274:289	body composition	274:289	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	12	50	from	levels	1599:1604	arg1	period					1686:1691	a chronic period	1676:1691	a chronic period	1676:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	11	51	theme	body	1430:1433	arg1	composition					1435:1445	body composition	1430:1445	body composition	1430:1445	RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional.
35485727	12	52	theme	reduced	1591:1597	arg1	levels					1599:1604	reduced levels	1591:1604	reduced levels of claudin-1 and occludin transcripts	1591:1642	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	11	53	theme	dynamic	1477:1483	arg1	changes					1485:1491	dynamic changes	1477:1491	dynamic changes in intestinal barrier morphofunctional	1477:1530	RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional.
35485727	1	54	theme	Few	178:180	arg1	studies					182:188	Few studies	178:188	Few studies	178:188	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	4	55	theme	morphometric	642:653	arg1	analysis					655:662	morphometric analysis	642:662	morphometric analysis	642:662	The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters.
35485727	5	56	theme	tail	855:858	arg1	length					860:865	tail length	855:865	tail length from day 14	855:877	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	1	57	theme	associated	235:244	arg1	dynamics					262:269	associated pathobiological dynamics	235:269	associated pathobiological dynamics of body composition and intestinal barrier function	235:321	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	0	58	theme	impaired	126:133	arg1	development					144:154	impaired physical development	126:154	impaired physical development	126:154	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	5	59	theme	weight	828:833	arg1	gain					835:838	weight gain	828:838	weight gain from day 3	828:849	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	6	60	theme	extracellular	924:936	arg1	fluid					938:942	extracellular fluid	924:942	extracellular fluid	924:942	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	3	61	theme	basic	528:532	arg1	diet					534:537	a Northeastern Brazil regional basic diet	497:537	a Northeastern Brazil regional basic diet (RBD)	497:543	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	3	61	theme	basic	528:532	arg1	RBD					540:542	RBD	540:542	RBD	540:542	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	2	62	theme	nutrient-deficient	361:378	arg1	diet					380:383	a nutrient-deficient diet	359:383	a nutrient-deficient diet	359:383	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	0	63	theme	post-weaning	159:170	arg1	mice					172:175	post-weaning mice	159:175	post-weaning mice	159:175	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	5	64	from	day	845:847	arg1	0·05					810:813	P < 0·05	806:813	P < 0·05	806:813	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	64	from	day	845:847	arg1	weight					798:803	weight	798:803	weight (P < 0·05) from day 5	798:825	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	64	from	day	845:847	arg1	gain					835:838	weight gain	828:838	weight gain from day 3	828:849	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	64	from	day	845:847	arg1	length					860:865	tail length	855:865	tail length from day 14	855:877	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	6	65	theme	total	906:910	arg1	water					917:921	total body water	906:921	total body water	906:921	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	7	66	from	reduction	1096:1104	arg1	0·05					1129:1132	P < 0·05	1125:1132	P < 0·05	1125:1132	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	7	66	from	reduction	1096:1104	arg1	days					1109:1112	days 14 and 21	1109:1122	days	1109:1112	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	7	66	from	reduction	1096:1104	arg1	21					1121:1122	21	1121:1122	21	1121:1122	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	4	67	theme	tight	719:723	arg1	junctions					725:733	tight junctions	719:733	tight junctions	719:733	The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters.
35485727	8	68	theme	mannitol	1146:1153	arg1	ratio					1155:1159	Lactulose:mannitol ratio	1136:1159	Lactulose:mannitol ratio	1136:1159	Lactulose:mannitol ratio increased on day 14 (P < 0·05).
35485727	4	69	theme	reverse	681:687	arg1	transcription-PCR					689:705	quantitative reverse transcription-PCR	668:705	quantitative reverse transcription-PCR	668:705	The animals were subjected to bioimpedance analysis, lactulose test, morphometric analysis and quantitative reverse transcription-PCR to evaluate tight junctions and intestinal transporters.
35485727	0	70	theme	morphofunctional	79:94	arg1	barrier					96:102	the intestinal morphofunctional barrier	64:102	the intestinal morphofunctional barrier	64:102	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	9	71	from	Changes	1193:1199	arg1	function					1223:1230	intestinal barrier function	1204:1230	intestinal barrier function on day 14	1204:1240	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	9	71	from	Changes	1193:1199	arg1	day					1235:1237	day 14	1235:1240	day 14	1235:1240	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	6	72	theme	fat-free	958:965	arg1	mass					967:970	fat-free mass	958:970	fat-free mass	958:970	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	0	73	from	changes	53:59	arg1	barrier					96:102	the intestinal morphofunctional barrier	64:102	the intestinal morphofunctional barrier	64:102	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	5	74	theme	RBD	764:766	arg1	feeding					768:774	RBD feeding	764:774	RBD feeding	764:774	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	75	from	day	872:874	arg1	0·05					810:813	P < 0·05	806:813	P < 0·05	806:813	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	75	from	day	872:874	arg1	weight					798:803	weight	798:803	weight (P < 0·05) from day 5	798:825	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	75	from	day	872:874	arg1	gain					835:838	weight gain	828:838	weight gain from day 3	828:849	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	75	from	day	872:874	arg1	length					860:865	tail length	855:865	tail length from day 14	855:877	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	8	76	theme	<	1184:1184	arg1	0·05					1186:1189	P < 0·05	1182:1189	P < 0·05	1182:1189	Lactulose:mannitol ratio increased on day 14 (P < 0·05).
35485727	8	76	theme	<	1184:1184	arg1	day					1174:1176	day 14	1174:1179	day 14 (P < 0·05)	1174:1190	Lactulose:mannitol ratio increased on day 14 (P < 0·05).
35485727	3	77	theme	age	478:480	arg1	days					470:473	21 days	467:473	21 days of age	467:480	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	5	78	from	day	821:823	arg1	0·05					810:813	P < 0·05	806:813	P < 0·05	806:813	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	78	from	day	821:823	arg1	weight					798:803	weight	798:803	weight (P < 0·05) from day 5	798:825	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	78	from	day	821:823	arg1	gain					835:838	weight gain	828:838	weight gain from day 3	828:849	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	5	78	from	day	821:823	arg1	length					860:865	tail length	855:865	tail length from day 14	855:877	RBD feeding significantly reduced weight (P < 0·05) from day 5, weight gain from day 3 and tail length from day 14.
35485727	9	79	theme	claudin-2	1355:1363	arg1	levels					1345:1350	the levels	1341:1350	the levels of claudin-2 and occludin	1341:1376	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	7	80	from	changes	1007:1013	arg1	jejunum					1022:1028	the jejunum	1018:1028	the jejunum	1018:1028	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	2	81	theme	intestinal	413:422	arg1	barrier					441:447	intestinal morphofunctional barrier	413:447	intestinal morphofunctional barrier	413:447	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	0	82	theme	multi-deficient	17:31	arg1	diet					33:36	a multi-deficient diet	15:36	a multi-deficient diet	15:36	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	1	83	theme	function	314:321	arg1	dynamics					262:269	associated pathobiological dynamics	235:269	associated pathobiological dynamics of body composition and intestinal barrier function	235:321	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	1	83	theme	function	314:321	arg1	diets					225:229	nutrient-deficient diets	206:229	nutrient-deficient diets	206:229	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	12	84	theme	chronic	1678:1684	arg1	period					1686:1691	a chronic period	1676:1691	a chronic period	1676:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	9	85	theme	occludin	1369:1376	arg1	levels					1345:1350	the levels	1341:1350	the levels of claudin-2 and occludin	1341:1376	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	11	86	theme	intestinal	1496:1505	arg1	barrier					1507:1513	intestinal barrier morphofunctional	1496:1530	intestinal barrier morphofunctional	1496:1530	RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional.
35485727	7	87	theme	villus	1055:1060	arg1	ratio					1068:1072	the villus:crypt ratio	1051:1072	the villus:crypt ratio on day 7	1051:1081	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	9	88	from	day	1235:1237	arg1	Changes					1193:1199	Changes	1193:1199	Changes in intestinal barrier function on day 14	1193:1240	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	6	89	theme	P	984:984	arg1	0·05					988:991	P < 0·05	984:991	P < 0·05	984:991	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	6	89	theme	P	984:984	arg1	day					977:979	day 7	977:981	day 7 (P < 0·05)	977:992	The intake of RBD reduced total body water, extracellular fluid, fat mass and fat-free mass from day 7 (P < 0·05).
35485727	1	90	theme	pathobiological	246:260	arg1	dynamics					262:269	associated pathobiological dynamics	235:269	associated pathobiological dynamics of body composition and intestinal barrier function	235:321	Few studies have focused on nutrient-deficient diets and associated pathobiological dynamics of body composition and intestinal barrier function.
35485727	9	91	from	function	1223:1230	arg1	day					1235:1237	day 14	1235:1240	day 14	1235:1240	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	12	92	theme	occludin	1623:1630	arg1	transcripts					1632:1642	claudin-1 and occludin transcripts	1609:1642	claudin-1 and occludin transcripts	1609:1642	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	12	93	from	return	1648:1653	arg1	period					1686:1691	a chronic period	1676:1691	a chronic period	1676:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485727	3	94	theme	regional	519:526	arg1	diet					534:537	a Northeastern Brazil regional basic diet	497:537	a Northeastern Brazil regional basic diet (RBD)	497:543	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	3	94	theme	regional	519:526	arg1	RBD					540:542	RBD	540:542	RBD	540:542	C57BL/6 (21 days of age) mice were fed a Northeastern Brazil regional basic diet (RBD) or a control diet for 21 d.
35485727	2	95	from	barrier	441:447	arg1	mice					452:455	mice	452:455	mice	452:455	This study evaluated the impact of a nutrient-deficient diet on physical development and intestinal morphofunctional barrier in mice.
35485727	7	96	theme	<	1127:1127	arg1	0·05					1129:1132	P < 0·05	1125:1132	P < 0·05	1125:1132	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	7	96	theme	<	1127:1127	arg1	days					1109:1112	days 14 and 21	1109:1122	days	1109:1112	RBD induced changes in the jejunum, with an increase in the villus:crypt ratio on day 7, followed by reduction on days 14 and 21 (P < 0·05).
35485727	0	97	from	development	144:154	arg1	barrier					96:102	the intestinal morphofunctional barrier	64:102	the intestinal morphofunctional barrier	64:102	Consumption of a multi-deficient diet causes dynamic changes in the intestinal morphofunctional barrier, body composition and impaired physical development in post-weaning mice.
35485727	11	98	theme	morphofunctional	1515:1530	arg1	barrier					1507:1513	intestinal barrier morphofunctional	1496:1530	intestinal barrier morphofunctional	1496:1530	RBD compromises body composition and physical development with dynamic changes in intestinal barrier morphofunctional.
35485727	9	99	theme	barrier	1215:1221	arg1	function					1223:1230	intestinal barrier function	1204:1230	intestinal barrier function on day 14	1204:1240	Changes in intestinal barrier function on day 14 were associated with reductions in claudin-1 and occludin, and on day 21, there was a reduction in the levels of claudin-2 and occludin.
35485727	12	100	from	function	1664:1671	arg1	period					1686:1691	a chronic period	1676:1691	a chronic period	1676:1691	RBD is associated with damage to intestinal permeability, reduced levels of claudin-1 and occludin transcripts and return of bowel function in a chronic period.
35485815	7	0	theme	patients	1412:1419	arg1	examination					1397:1407	The examination	1393:1407	The examination of patients	1393:1419	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	11	1	from	0.44+/-0.10	2482:2492	arg1	times					2455:2459	2.8 times	2451:2459	2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05	2451:2525	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	2	2	from	pregnancy	499:507	arg1	cycle					524:528	the natural cycle	512:528	the natural cycle	512:528	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	1	3	from	men	284:286	arg1	disorder					220:227	a disorder	218:227	a disorder	218:227	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	3	4	dep	LTD	972:974	arg1	Novosibirsk					977:987	Novosibirsk	977:987	Novosibirsk	977:987	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	15	5	theme	main	3140:3143	arg1	characteristics					3145:3159	the main characteristics	3136:3159	the main characteristics of spermatozoa	3136:3174	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	5	theme	main	3140:3143	arg1	parameters					3075:3084	the parameters	3071:3084	the parameters of the ejaculate	3071:3101	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	6	theme	"	2917:2917	arg1	use					2894:2896	the use	2890:2896	the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	2890:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	7	theme	chronic	2931:2937	arg1	prostatitis					2939:2949	chronic prostatitis	2931:2949	chronic prostatitis associated with secondary infertility	2931:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	1	8	from	functions	271:279	arg1	men					284:286	men	284:286	men	284:286	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	1	9	dep	oligoasthenoteratozoospermia	419:446	arg1	up					413:414	up	413:414	up	413:414	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	15	10	theme	ANDROEXPERT	2902:2912	arg1	"					2917:2917	"ANDROEXPERT SV1"	2901:2917	"ANDROEXPERT SV1"	2901:2917	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	7	11	theme	09.11.2012	1688:1697	arg1	775n					1680:1683	Russia No. 775n	1669:1683	Russia No. 775n of 09.11.2012	1669:1697	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	8	12	dep	groups	1736:1741	arg1	received					1770:1777	received	1770:1777	received oxidised dextran rectally once per day for 10 days, then 20 days every other day	1770:1858	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	16	13	theme	ANDROEXPERT	3183:3193	arg1	suppositories					3199:3211	ANDROEXPERT SV1 suppositories	3183:3211	ANDROEXPERT SV1 suppositories	3183:3211	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	15	14	from	men	2922:2924	arg1	result					2880:2885	a result	2878:2885	a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	2878:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	14	from	men	2922:2924	arg1	dynamics					2841:2848	the dynamics	2837:2848	the dynamics of spermogram parameters	2837:2873	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	8	15	theme	patients	1761:1768	arg1	group					1752:1756	the 1st group	1744:1756	the 1st group of patients	1744:1768	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	8	15	theme	patients	1761:1768	arg1	patients					1761:1768	patients	1761:1768	patients	1761:1768	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	1	16	theme	spermogram	390:399	arg1	parameters					401:410	spermogram parameters	390:410	spermogram parameters	390:410	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	2	17	with	impossible	533:542	arg1	function					592:599	a completely preserved reproductive function	556:599	a completely preserved reproductive function in a woman	556:610	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	15	18	from	use	2894:2896	arg1	men					2922:2924	men	2922:2924	men with chronic prostatitis associated with secondary infertility	2922:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	7	19	theme	775n	1680:1683	arg1	Health					1659:1664	Health	1659:1664	Health of Russia No. 775n of 09.11.2012	1659:1697	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	14	20	from	spermogram	2705:2714	arg1	patients					2719:2726	patients	2719:2726	patients with chronic prostatitis of the second group	2719:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	3	21	theme	chronic	664:670	arg1	prostatitis					672:682	chronic prostatitis	664:682	chronic prostatitis associated with secondary infertility	664:720	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	8	22	theme	oxidised	1779:1786	arg1	dextran					1788:1794	oxidised dextran	1779:1794	oxidised dextran	1779:1794	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	7	23	theme	Russia	1669:1674	arg1	775n					1680:1683	Russia No. 775n	1669:1683	Russia No. 775n of 09.11.2012	1669:1697	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	4	24	theme	study	1017:1021	arg1	purpose					1002:1008	The purpose	998:1008	The purpose of the study	998:1021	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	16	25	theme	high	3218:3221	arg1	efficacy					3232:3239	high clinical efficacy	3218:3239	high clinical efficacy	3218:3239	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	3	26	theme	men	655:657	arg1	recovery					643:650	the recovery	639:650	the recovery of men with chronic prostatitis associated with secondary infertility	639:720	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	14	27	theme	second	2760:2765	arg1	group					2767:2771	the second group	2756:2771	the second group	2756:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	15	28	theme	ejaculate	3093:3101	arg1	properties					3121:3130	its general properties	3109:3130	its general properties	3109:3130	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	28	theme	ejaculate	3093:3101	arg1	characteristics					3145:3159	the main characteristics	3136:3159	the main characteristics of spermatozoa	3136:3174	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	28	theme	ejaculate	3093:3101	arg1	parameters					3075:3084	the parameters	3071:3084	the parameters of the ejaculate	3071:3101	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	14	29	with	patients	2719:2726	arg1	prostatitis					2741:2751	chronic prostatitis	2733:2751	chronic prostatitis of the second group	2733:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	4	30	with	men	1160:1162	arg1	prostatitis					1177:1187	chronic prostatitis	1169:1187	chronic prostatitis associated with secondary infertility	1169:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	3	31	theme	secondary	700:708	arg1	infertility					710:720	secondary infertility	700:720	secondary infertility	700:720	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	0	32	theme	ANDROEXPERT	120:130	arg1	suppositories					136:148	ANDROEXPERT SV1 suppositories	120:148	ANDROEXPERT SV1 suppositories	120:148	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	9	33	dep	RESULTS	1988:1994	arg1	increased					2052:2060	increased	2052:2060	increased from 2.54+/-0.15 to 3.13+/-0.18 (ml), which amounted to 23.23%; p<0.05	2052:2131	RESULTS The volume of ejaculate among patients of the 1st group increased from 2.54+/-0.15 to 3.13+/-0.18 (ml), which amounted to 23.23%; p<0.05.
35485815	7	34	dep	prostatitis	1605:1615	arg1	Appendix					1618:1625	Appendix	1618:1625	Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012	1618:1697	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	3	35	from	substance	626:634	arg1	recovery					643:650	the recovery	639:650	the recovery of men with chronic prostatitis associated with secondary infertility	639:720	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	0	36	theme	suppositories	136:148	arg1	use					113:115	the use	109:115	the use of ANDROEXPERT SV1 suppositories	109:148	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	1	37	theme	ejaculate	341:349	arg1	quality					326:332	the quality	322:332	the quality of the ejaculate	322:349	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	3	38	theme	pharmaceutical	769:782	arg1	composition					784:794	a pharmaceutical composition	767:794	a pharmaceutical composition	767:794	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	7	39	theme	European	1521:1528	arg1	Association					1541:1551	the European Urological Association	1517:1551	the European Urological Association	1517:1551	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	2	40	theme	pregnancy	499:507	arg1	impossible					533:542	impossible	533:542	impossible	533:542	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	2	40	theme	pregnancy	499:507	arg1	onset					490:494	the onset	486:494	the onset of pregnancy in the natural cycle	486:528	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	4	41	theme	use	1096:1098	arg1	dynamics					1043:1050	the dynamics	1039:1050	the dynamics of spermogram parameters	1039:1075	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	4	41	theme	use	1096:1098	arg1	result					1082:1087	a result	1080:1087	a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1080:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	2	42	from	function	592:599	arg1	woman					606:610	a woman	604:610	a woman	604:610	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	10	43	theme	concentration	2144:2156	arg1	indicator					2158:2166	The sperm concentration indicator	2134:2166	The sperm concentration indicator	2134:2166	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	9	44	theme	1st	2042:2044	arg1	group					2046:2050	the 1st group	2038:2050	the 1st group	2038:2050	RESULTS The volume of ejaculate among patients of the 1st group increased from 2.54+/-0.15 to 3.13+/-0.18 (ml), which amounted to 23.23%; p<0.05.
35485815	10	45	from	increase	2262:2269	arg1	number					2284:2289	the total number	2274:2289	the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05	2274:2392	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	12	46	theme	agglutination	2567:2579	arg1	severity					2549:2556	the severity	2545:2556	the severity of sperm agglutination	2545:2579	The indicator of the severity of sperm agglutination decreased by 3 times: from 0.52+/-0.07 to 0.17+/-0.03 - by 0.35 arb.
35485815	4	47	theme	parameters	1066:1075	arg1	dynamics					1043:1050	the dynamics	1039:1050	the dynamics of spermogram parameters	1039:1075	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	4	47	theme	parameters	1066:1075	arg1	result					1082:1087	a result	1080:1087	a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1080:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	3	48	theme	composition	784:794	arg1	form					759:762	the form	755:762	the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia	755:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	8	49	theme	comparison	1870:1879	arg1	2nd					1865:1867	2nd	1865:1867	2nd	1865:1867	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	8	49	theme	comparison	1870:1879	arg1	group					1881:1885	comparison group	1870:1885	comparison group	1870:1885	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	15	50	from	result	2880:2885	arg1	men					2922:2924	men	2922:2924	men with chronic prostatitis associated with secondary infertility	2922:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	10	51	from	number	2284:2289	arg1	ejaculate					2313:2321	the ejaculate	2309:2321	the ejaculate by 88.5%	2309:2330	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	10	51	from	number	2284:2289	arg1	pieces					2378:2383	million pieces	2370:2383	million pieces	2370:2383	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	10	51	from	number	2284:2289	arg1	123.41+/-12.33					2354:2367	123.41+/-12.33	2354:2367	123.41+/-12.33	2354:2367	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	15	52	theme	leukocytes	3027:3036	arg1	number					3017:3022	the number	3013:3022	the number of leukocytes	3013:3036	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	0	53	from	[Dynamics	0:8	arg1	men					33:35	men	33:35	men with chronic prostatitis	33:60	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	2	54	theme	reproductive	579:590	arg1	function					592:599	a completely preserved reproductive function	556:599	a completely preserved reproductive function in a woman	556:610	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	8	55	theme	other	1850:1854	arg1	day					1856:1858	every other day	1844:1858	every other day	1844:1858	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	3	56	theme	Research	828:835	arg1	Centre					837:842	Federal Research Centre	820:842	Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia	820:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	11	57	theme	same	2427:2430	arg1	group					2432:2436	the same group	2423:2436	the same group	2423:2436	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	4	58	theme	SV1	1152:1154	arg1	"					1155:1155	oxidised dextran "ANDROEXPERT SV1"	1122:1155	oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1122:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	16	59	theme	management	3306:3315	arg1	tactics					3317:3323	the management tactics	3302:3323	the management tactics for this group of patients	3302:3350	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	3	60	with	men	655:657	arg1	prostatitis					672:682	chronic prostatitis	664:682	chronic prostatitis associated with secondary infertility	664:720	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	9	61	theme	group	2046:2050	arg1	patients					2026:2033	patients	2026:2033	patients of the 1st group	2026:2050	RESULTS The volume of ejaculate among patients of the 1st group increased from 2.54+/-0.15 to 3.13+/-0.18 (ml), which amounted to 23.23%; p<0.05.
35485815	7	62	theme	chronic	1597:1603	arg1	prostatitis					1605:1615	chronic prostatitis	1597:1615	chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012)	1597:1698	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	4	63	theme	suppositories	1103:1115	arg1	use					1096:1098	the use	1092:1098	the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1092:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	1	64	theme	sexual	241:246	arg1	functions					271:279	not only sexual, but also reproductive functions	232:279	not only sexual, but also reproductive functions in men	232:286	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	10	65	dep	%	2235:2235	arg1	p<0.05					2238:2243	p<0.05	2238:2243	55.75%; p<0.05	2230:2243	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	7	66	theme	care	1588:1591	arg1	standard					1561:1568	standard	1561:1568	standard	1561:1568	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	3	67	from	dextran	744:750	arg1	form					759:762	the form	755:762	the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia	755:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	68	theme	ANDROEXPERT	925:935	arg1	Research					937:944	ANDROEXPERT Research	925:944	ANDROEXPERT Research	925:944	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	10	69	theme	million	2370:2376	arg1	123.41+/-12.33					2354:2367	123.41+/-12.33	2354:2367	123.41+/-12.33	2354:2367	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	10	69	theme	million	2370:2376	arg1	pieces					2378:2383	million pieces	2370:2383	million pieces	2370:2383	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	0	70	theme	sperm	13:17	arg1	indicators					19:28	sperm indicators	13:28	sperm indicators	13:28	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	3	71	theme	Fundamental	848:858	arg1	Novosibirsk					888:898	Novosibirsk	888:898	Novosibirsk	888:898	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	71	theme	Fundamental	848:858	arg1	Medicine					878:885	Fundamental and Translational Medicine	848:885	Fundamental and Translational Medicine	848:885	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	71	theme	Fundamental	848:858	arg1	Russia					901:906	Russia	901:906	Russia	901:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	7	72	theme	primary	1573:1579	arg1	care					1588:1591	primary health care	1573:1591	primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012)	1573:1698	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	8	73	theme	rectal	1922:1927	arg1	suppositories					1929:1941	rectal suppositories	1922:1941	rectal suppositories consisting of cocoa butter in a similar way	1922:1985	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	8	74	dep	day	1814:1816	arg1	day					1856:1858	every other day	1844:1858	every other day	1844:1858	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	1	75	theme	reproductive	258:269	arg1	functions					271:279	not only sexual, but also reproductive functions	232:279	not only sexual, but also reproductive functions in men	232:286	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	12	76	from	0.17+/-0.03	2623:2633	arg1	times					2596:2600	3 times	2594:2600	3 times: from 0.52+/-0.07 to 0.17+/-0.03 - by 0.35 arb	2594:2647	The indicator of the severity of sperm agglutination decreased by 3 times: from 0.52+/-0.07 to 0.17+/-0.03 - by 0.35 arb.
35485815	9	77	dep	3.13+/-0.18	2082:2092	arg1	to					2079:2080	to	2079:2080	to	2079:2080	RESULTS The volume of ejaculate among patients of the 1st group increased from 2.54+/-0.15 to 3.13+/-0.18 (ml), which amounted to 23.23%; p<0.05.
35485815	8	78	theme	cocoa	1957:1961	arg1	butter					1963:1968	cocoa butter	1957:1968	cocoa butter in a similar way	1957:1985	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	13	79	dep	p<0.05	2675:2680	arg1	units					2650:2654	units	2650:2654	units	2650:2654	units, which was 67.31%; p<0.05.
35485815	5	80	theme	secondary	1321:1329	arg1	infertility					1331:1341	secondary infertility	1321:1341	secondary infertility	1321:1341	MATERIAL AND METHODS The study included 52 patients with chronic prostatitis associated with secondary infertility.
35485815	1	81	theme	common	202:207	arg1	cause					209:213	the most common cause	193:213	the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia	193:462	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	1	81	theme	common	202:207	arg1	INTRODUCTION					152:163	INTRODUCTION Chronic prostatitis (CP)	152:188	INTRODUCTION Chronic prostatitis (CP)	152:188	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	5	82	dep	MATERIAL	1228:1235	arg1	study					1253:1257	The study	1249:1257	The study	1249:1257	MATERIAL AND METHODS The study included 52 patients with chronic prostatitis associated with secondary infertility.
35485815	15	83	from	decrease	3001:3008	arg1	properties					3121:3130	its general properties	3109:3130	its general properties	3109:3130	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	83	from	decrease	3001:3008	arg1	number					3017:3022	the number	3013:3022	the number of leukocytes	3013:3036	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	83	from	decrease	3001:3008	arg1	characteristics					3145:3159	the main characteristics	3136:3159	the main characteristics of spermatozoa	3136:3174	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	83	from	decrease	3001:3008	arg1	parameters					3075:3084	the parameters	3071:3084	the parameters of the ejaculate	3071:3101	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	10	84	from	123.41+/-12.33	2354:2367	arg1	spermatozoa					2294:2304	spermatozoa	2294:2304	spermatozoa	2294:2304	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	10	84	from	123.41+/-12.33	2354:2367	arg1	number					2284:2289	the total number	2274:2289	the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05	2274:2392	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	2	85	from	situation	475:483	arg1	onset					490:494	the onset	486:494	the onset of pregnancy in the natural cycle	486:528	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	2	85	from	situation	475:483	arg1	impossible					533:542	impossible	533:542	impossible	533:542	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	8	86	theme	similar	1975:1981	arg1	way					1983:1985	a similar way	1973:1985	a similar way	1973:1985	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	14	87	theme	chronic	2733:2739	arg1	prostatitis					2741:2751	chronic prostatitis	2733:2751	chronic prostatitis of the second group	2733:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	8	88	theme	1st	1748:1750	arg1	group					1752:1756	the 1st group	1744:1756	the 1st group of patients	1744:1768	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	8	88	theme	1st	1748:1750	arg1	patients					1761:1768	patients	1761:1768	patients	1761:1768	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	5	89	theme	chronic	1285:1291	arg1	prostatitis					1293:1303	chronic prostatitis	1285:1303	chronic prostatitis associated with secondary infertility	1285:1341	MATERIAL AND METHODS The study included 52 patients with chronic prostatitis associated with secondary infertility.
35485815	10	90	dep	123.41+/-12.33	2354:2367	arg1	to					2351:2352	to	2351:2352	to	2351:2352	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	10	90	dep	123.41+/-12.33	2354:2367	arg1	p<0.05					2387:2392	p<0.05	2387:2392	p<0.05	2387:2392	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	14	91	theme	treatment	2805:2813	arg1	course					2795:2800	the course	2791:2800	the course of treatment	2791:2813	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	1	92	theme	disorder	220:227	arg1	cause					209:213	the most common cause	193:213	the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia	193:462	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	1	92	theme	disorder	220:227	arg1	INTRODUCTION					152:163	INTRODUCTION Chronic prostatitis (CP)	152:188	INTRODUCTION Chronic prostatitis (CP)	152:188	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	15	93	theme	spermatozoa	3164:3174	arg1	parameters					3075:3084	the parameters	3071:3084	the parameters of the ejaculate	3071:3101	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	93	theme	spermatozoa	3164:3174	arg1	characteristics					3145:3159	the main characteristics	3136:3159	the main characteristics of spermatozoa	3136:3174	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	93	theme	spermatozoa	3164:3174	arg1	properties					3121:3130	its general properties	3109:3130	its general properties	3109:3130	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	94	theme	SV1	2914:2916	arg1	"					2917:2917	"ANDROEXPERT SV1"	2901:2917	"ANDROEXPERT SV1"	2901:2917	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	95	with	men	2922:2924	arg1	prostatitis					2939:2949	chronic prostatitis	2931:2949	chronic prostatitis associated with secondary infertility	2931:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	14	96	from	patients	2719:2726	arg1	indicators					2687:2696	The indicators	2683:2696	The indicators of the spermogram in patients with chronic prostatitis of the second group	2683:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	15	97	from	improvement	3056:3066	arg1	properties					3121:3130	its general properties	3109:3130	its general properties	3109:3130	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	97	from	improvement	3056:3066	arg1	number					3017:3022	the number	3013:3022	the number of leukocytes	3013:3036	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	97	from	improvement	3056:3066	arg1	characteristics					3145:3159	the main characteristics	3136:3159	the main characteristics of spermatozoa	3136:3174	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	97	from	improvement	3056:3066	arg1	parameters					3075:3084	the parameters	3071:3084	the parameters of the ejaculate	3071:3101	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	2	98	theme	natural	516:522	arg1	cycle					524:528	the natural cycle	512:528	the natural cycle	512:528	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	8	99	from	butter	1963:1968	arg1	way					1983:1985	a similar way	1973:1985	a similar way	1973:1985	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	15	100	theme	parameters	2864:2873	arg1	result					2880:2885	a result	2878:2885	a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	2878:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	100	theme	parameters	2864:2873	arg1	dynamics					2841:2848	the dynamics	2837:2848	the dynamics of spermogram parameters	2837:2873	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	1	101	theme	Chronic	165:171	arg1	CP					186:187	CP	186:187	CP	186:187	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	1	101	theme	Chronic	165:171	arg1	prostatitis					173:183	Chronic prostatitis	165:183	INTRODUCTION Chronic prostatitis (CP)	152:188	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	15	102	theme	use	2894:2896	arg1	result					2880:2885	a result	2878:2885	a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	2878:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	102	theme	use	2894:2896	arg1	dynamics					2841:2848	the dynamics	2837:2848	the dynamics of spermogram parameters	2837:2873	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	7	103	theme	No.	1676:1678	arg1	775n					1680:1683	Russia No. 775n	1669:1683	Russia No. 775n of 09.11.2012	1669:1697	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	4	104	theme	spermogram	1055:1064	arg1	parameters					1066:1075	spermogram parameters	1055:1075	spermogram parameters	1055:1075	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	3	105	theme	Manufacturing	950:962	arg1	Company					964:970	Manufacturing Company	950:970	Manufacturing Company	950:970	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	12	106	dep	0.17+/-0.03	2623:2633	arg1	to					2620:2621	to	2620:2621	to	2620:2621	The indicator of the severity of sperm agglutination decreased by 3 times: from 0.52+/-0.07 to 0.17+/-0.03 - by 0.35 arb.
35485815	16	107	theme	SV1	3195:3197	arg1	suppositories					3199:3211	ANDROEXPERT SV1 suppositories	3183:3211	ANDROEXPERT SV1 suppositories	3183:3211	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	11	108	from	group	2432:2436	arg1	number					2399:2404	The number	2395:2404	The number of leukocytes in the same group	2395:2436	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	14	109	theme	group	2767:2771	arg1	prostatitis					2741:2751	chronic prostatitis	2733:2751	chronic prostatitis of the second group	2733:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	9	110	theme	ejaculate	2010:2018	arg1	volume					2000:2005	The volume	1996:2005	The volume of ejaculate	1996:2018	RESULTS The volume of ejaculate among patients of the 1st group increased from 2.54+/-0.15 to 3.13+/-0.18 (ml), which amounted to 23.23%; p<0.05.
35485815	1	111	from	disorder	220:227	arg1	men					284:286	men	284:286	men	284:286	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	7	112	theme	Urological	1530:1539	arg1	Association					1541:1551	the European Urological Association	1517:1551	the European Urological Association	1517:1551	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	16	113	theme	clinical	3223:3230	arg1	efficacy					3232:3239	high clinical efficacy	3218:3239	high clinical efficacy	3218:3239	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	4	114	with	suppositories	1103:1115	arg1	"					1155:1155	oxidised dextran "ANDROEXPERT SV1"	1122:1155	oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1122:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	11	115	dep	0.44+/-0.10	2482:2492	arg1	to					2479:2480	to	2479:2480	to	2479:2480	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	10	116	from	spermatozoa	2294:2304	arg1	ejaculate					2313:2321	the ejaculate	2309:2321	the ejaculate by 88.5%	2309:2330	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	7	117	theme	Health	1659:1664	arg1	Ministry					1647:1654	the Ministry	1643:1654	the Ministry of Health of Russia No. 775n of 09.11.2012	1643:1697	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	10	118	theme	sperm	2138:2142	arg1	indicator					2158:2166	The sperm concentration indicator	2134:2166	The sperm concentration indicator	2134:2166	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	9	119	dep	%	2123:2123	arg1	p<0.05					2126:2131	p<0.05	2126:2131	23.23%; p<0.05	2118:2131	RESULTS The volume of ejaculate among patients of the 1st group increased from 2.54+/-0.15 to 3.13+/-0.18 (ml), which amounted to 23.23%; p<0.05.
35485815	10	120	theme	spermatozoa	2294:2304	arg1	number					2284:2289	the total number	2274:2289	the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05	2274:2392	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	3	121	theme	oxidised	735:742	arg1	substance					626:634	an active substance	616:634	an active substance in the recovery of men with chronic prostatitis associated with secondary infertility	616:720	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	121	theme	oxidised	735:742	arg1	dextran					744:750	oxidised dextran	735:750	oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia	735:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	7	122	theme	Ministry	1647:1654	arg1	order					1634:1638	the order	1630:1638	the order of the Ministry of Health of Russia No. 775n of 09.11.2012	1630:1697	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	12	123	theme	sperm	2561:2565	arg1	agglutination					2567:2579	sperm agglutination	2561:2579	sperm agglutination	2561:2579	The indicator of the severity of sperm agglutination decreased by 3 times: from 0.52+/-0.07 to 0.17+/-0.03 - by 0.35 arb.
35485815	0	124	theme	secondary	78:86	arg1	infertility					88:98	secondary infertility	78:98	secondary infertility based on the use of ANDROEXPERT SV1 suppositories	78:148	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	11	125	from	number	2399:2404	arg1	group					2432:2436	the same group	2423:2436	the same group	2423:2436	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	15	126	theme	significant	3044:3054	arg1	improvement					3056:3066	a significant improvement	3042:3066	a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa	3042:3174	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	4	127	theme	ANDROEXPERT	1140:1150	arg1	"					1155:1155	oxidised dextran "ANDROEXPERT SV1"	1122:1155	oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1122:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	11	128	from	leukocytes	2409:2418	arg1	group					2432:2436	the same group	2423:2436	the same group	2423:2436	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	10	129	from	ejaculate	2313:2321	arg1	number					2284:2289	the total number	2274:2289	the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05	2274:2392	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	4	130	from	"	1155:1155	arg1	men					1160:1162	men	1160:1162	men with chronic prostatitis associated with secondary infertility	1160:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	4	131	theme	oxidised	1122:1129	arg1	"					1155:1155	oxidised dextran "ANDROEXPERT SV1"	1122:1155	oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1122:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	2	132	theme	preserved	569:577	arg1	function					592:599	a completely preserved reproductive function	556:599	a completely preserved reproductive function in a woman	556:610	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	14	133	theme	spermogram	2705:2714	arg1	indicators					2687:2696	The indicators	2683:2696	The indicators of the spermogram in patients with chronic prostatitis of the second group	2683:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	15	134	theme	general	3113:3119	arg1	parameters					3075:3084	the parameters	3071:3084	the parameters of the ejaculate	3071:3101	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	15	134	theme	general	3113:3119	arg1	properties					3121:3130	its general properties	3109:3130	its general properties	3109:3130	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	12	135	theme	severity	2549:2556	arg1	indicator					2532:2540	The indicator	2528:2540	The indicator of the severity of sperm agglutination	2528:2579	The indicator of the severity of sperm agglutination decreased by 3 times: from 0.52+/-0.07 to 0.17+/-0.03 - by 0.35 arb.
35485815	1	136	from	decrease	310:317	arg1	quality					326:332	the quality	322:332	the quality of the ejaculate	322:349	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	3	137	theme	Federal	820:826	arg1	Centre					837:842	Federal Research Centre	820:842	Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia	820:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	11	138	dep	%	2517:2517	arg1	p<0.05					2520:2525	p<0.05	2520:2525	64.80%; p<0.05	2512:2525	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	0	139	theme	SV1	132:134	arg1	suppositories					136:148	ANDROEXPERT SV1 suppositories	120:148	ANDROEXPERT SV1 suppositories	120:148	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	2	140	from	cycle	524:528	arg1	impossible					533:542	impossible	533:542	impossible	533:542	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	2	140	from	cycle	524:528	arg1	onset					490:494	the onset	486:494	the onset of pregnancy in the natural cycle	486:528	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	4	141	theme	dextran	1131:1137	arg1	"					1155:1155	oxidised dextran "ANDROEXPERT SV1"	1122:1155	oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility	1122:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	1	142	dep	INTRODUCTION	152:163	arg1	CP					186:187	CP	186:187	CP	186:187	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	1	142	dep	INTRODUCTION	152:163	arg1	prostatitis					173:183	Chronic prostatitis	165:183	INTRODUCTION Chronic prostatitis (CP)	152:188	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	0	143	theme	indicators	19:28	arg1	[Dynamics					0:8	[Dynamics	0:8	[Dynamics of sperm indicators in men with chronic prostatitis	0:60	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	16	144	theme	patients	3343:3350	arg1	patients					3343:3350	patients	3343:3350	patients	3343:3350	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	16	144	theme	patients	3343:3350	arg1	group					3334:3338	this group	3329:3338	this group of patients	3329:3350	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	3	145	theme	Translational	864:876	arg1	Novosibirsk					888:898	Novosibirsk	888:898	Novosibirsk	888:898	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	145	theme	Translational	864:876	arg1	Medicine					878:885	Fundamental and Translational Medicine	848:885	Fundamental and Translational Medicine	848:885	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	145	theme	Translational	864:876	arg1	Russia					901:906	Russia	901:906	Russia	901:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	8	146	theme	suppositories	1929:1941	arg1	form					1914:1917	the form	1910:1917	the form of rectal suppositories consisting of cocoa butter in a similar way	1910:1985	The patients were divided into two groups: the 1st group of patients received oxidised dextran rectally once per day for 10 days, then 20 days every other day; The 2nd (comparison group) received a placebo in the form of rectal suppositories consisting of cocoa butter in a similar way.
35485815	2	147	from	impossible	533:542	arg1	situation					475:483	such a situation	468:483	such a situation	468:483	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	6	148	theme	mean	1348:1351	arg1	years					1386:1390	32+/-2.2 years	1377:1390	32+/-2.2 years	1377:1390	The mean age of the patients was 32+/-2.2 years.
35485815	6	148	theme	mean	1348:1351	arg1	age					1353:1355	The mean age	1344:1355	The mean age of the patients	1344:1371	The mean age of the patients was 32+/-2.2 years.
35485815	14	149	from	indicators	2687:2696	arg1	patients					2719:2726	patients	2719:2726	patients with chronic prostatitis of the second group	2719:2771	The indicators of the spermogram in patients with chronic prostatitis of the second group did not change in the course of treatment.
35485815	10	150	theme	total	2278:2282	arg1	number					2284:2289	the total number	2274:2289	the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05	2274:2392	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	7	151	theme	health	1581:1586	arg1	care					1588:1591	primary health care	1573:1591	primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012)	1573:1698	The examination of patients was carried out in accordance with the algorithm recommended by the Russian Society of Urology, the European Urological Association and the standard of primary health care for chronic prostatitis (Appendix to the order of the Ministry of Health of Russia No. 775n of 09.11.2012).
35485815	11	152	theme	leukocytes	2409:2418	arg1	number					2399:2404	The number	2395:2404	The number of leukocytes in the same group	2395:2436	The number of leukocytes in the same group decreased by 2.8 times: from 1.25+/-0.17 to 0.44+/-0.10 (million/ml) - by 64.80%; p<0.05.
35485815	4	153	theme	secondary	1205:1213	arg1	infertility					1215:1225	secondary infertility	1205:1225	secondary infertility	1205:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	0	154	theme	chronic	42:48	arg1	prostatitis					50:60	chronic prostatitis	42:60	chronic prostatitis	42:60	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	1	155	theme	functions	271:279	arg1	disorder					220:227	a disorder	218:227	a disorder	218:227	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	16	156	from	efficacy	3232:3239	arg1	monotherapy					3255:3265	monotherapy	3255:3265	monotherapy	3255:3265	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	10	157	dep	41.12+/-5.37	2199:2210	arg1	to					2196:2197	to	2196:2197	to	2196:2197	The sperm concentration indicator increased from 26.40+/-4.18 to 41.12+/-5.37 (million/ml) - by 55.75%; p<0.05, which led to an increase in the total number of spermatozoa in the ejaculate by 88.5%, from 65.47+/-8.41 to 123.41+/-12.33 (million pieces); p<0.05.
35485815	15	158	theme	secondary	2967:2975	arg1	infertility					2977:2987	secondary infertility	2967:2987	secondary infertility	2967:2987	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	4	159	theme	chronic	1169:1175	arg1	prostatitis					1177:1187	chronic prostatitis	1169:1187	chronic prostatitis associated with secondary infertility	1169:1225	The purpose of the study was to evaluate the dynamics of spermogram parameters as a result of the use of suppositories with oxidised dextran "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility.
35485815	3	160	dep	Research	937:944	arg1	Russia					990:995	Russia	990:995	Russia	990:995	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	160	dep	Research	937:944	arg1	LTD					972:974	LTD	972:974	LTD	972:974	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	16	161	from	safety	3245:3250	arg1	monotherapy					3255:3265	monotherapy	3255:3265	monotherapy	3255:3265	Thus, ANDROEXPERT SV1 suppositories show high clinical efficacy and safety in monotherapy and may be promising when choosing the management tactics for this group of patients.
35485815	15	162	theme	spermogram	2853:2862	arg1	parameters					2864:2873	spermogram parameters	2853:2873	spermogram parameters	2853:2873	CONCLUSION Assessing the dynamics of spermogram parameters as a result of the use of "ANDROEXPERT SV1" in men with chronic prostatitis associated with secondary infertility, there is a decrease in the number of leukocytes and a significant improvement in the parameters of the ejaculate, both its general properties and the main characteristics of spermatozoa.
35485815	1	163	from	deterioration	373:385	arg1	parameters					401:410	spermogram parameters	390:410	spermogram parameters	390:410	INTRODUCTION Chronic prostatitis (CP) is the most common cause of a disorder of not only sexual, but also reproductive functions in men, which is caused by a decrease in the quality of the ejaculate and is confirmed by a deterioration in spermogram parameters, up to oligoasthenoteratozoospermia and azoospermia.
35485815	2	164	from	onset	490:494	arg1	cycle					524:528	the natural cycle	512:528	the natural cycle	512:528	In such a situation, the onset of pregnancy in the natural cycle is impossible - even with a completely preserved reproductive function in a woman.
35485815	3	165	theme	active	619:624	arg1	substance					626:634	an active substance	616:634	an active substance in the recovery of men with chronic prostatitis associated with secondary infertility	616:720	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	3	165	theme	active	619:624	arg1	dextran					744:750	oxidised dextran	735:750	oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia	735:906	As an active substance in the recovery of men with chronic prostatitis associated with secondary infertility, we proposed oxidised dextran in the form of a pharmaceutical composition, which was developed at Federal Research Centre for Fundamental and Translational Medicine, Novosibirsk, Russia; and produced by ANDROEXPERT Research and Manufacturing Company LTD, Novosibirsk, Russia.
35485815	0	166	with	men	33:35	arg1	prostatitis					50:60	chronic prostatitis	42:60	chronic prostatitis	42:60	[Dynamics of sperm indicators in men with chronic prostatitis associated with secondary infertility based on the use of ANDROEXPERT SV1 suppositories].
35485815	6	167	theme	patients	1364:1371	arg1	years					1386:1390	32+/-2.2 years	1377:1390	32+/-2.2 years	1377:1390	The mean age of the patients was 32+/-2.2 years.
35485815	6	167	theme	patients	1364:1371	arg1	age					1353:1355	The mean age	1344:1355	The mean age of the patients	1344:1371	The mean age of the patients was 32+/-2.2 years.
35485815	5	168	with	patients	1271:1278	arg1	prostatitis					1293:1303	chronic prostatitis	1285:1303	chronic prostatitis associated with secondary infertility	1285:1341	MATERIAL AND METHODS The study included 52 patients with chronic prostatitis associated with secondary infertility.
35718141	7	0	theme	high-density	1424:1435	arg1	concentration					1461:1473	high-density lipoprotein-cholesterol concentration	1424:1473	high-density lipoprotein-cholesterol concentration	1424:1473	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	6	1	from	Patescibacteria	1101:1115	arg1	gut					1124:1126	the gut	1120:1126	the gut	1120:1126	GOS and SA also increased the abundance of Bacteroidota, Bifidobacterium, and Lactobacillus; and reduced that of Patescibacteria in the gut.
35718141	7	2	theme	correlation	1498:1508	arg1	analysis					1510:1517	Spearman correlation analysis	1489:1517	Spearman correlation analysis	1489:1517	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	0	3	theme	gut	108:110	arg1	microbiota					112:121	the gut microbiota	104:121	the gut microbiota	104:121	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	1	4	dep	metabolism	268:277	arg1	the					264:266	the	264:266	the	264:266	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	8	5	theme	associated	1573:1582	arg1	disorders					1594:1602	the associated metabolic disorders	1569:1602	the associated metabolic disorders	1569:1602	In conclusion, SA and GOS ameliorate obesity and the associated metabolic disorders in mice, and also modulate their gut microbial composition.
35718141	8	6	theme	microbial	1641:1649	arg1	composition					1651:1661	their gut microbial composition	1631:1661	their gut microbial composition	1631:1661	In conclusion, SA and GOS ameliorate obesity and the associated metabolic disorders in mice, and also modulate their gut microbial composition.
35718141	5	7	theme	D-lactic	823:830	arg1	D-LA					838:841	D-LA	838:841	D-LA	838:841	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	7	theme	D-lactic	823:830	arg1	acid					832:835	D-lactic acid	823:835	D-lactic acid (D-LA)	823:842	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	1	8	theme	obese	325:329	arg1	mice					331:334	high-fat diet (HFD)-fed obese mice	301:334	high-fat diet (HFD)-fed obese mice	301:334	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	3	9	theme	weight	508:513	arg1	gain					515:518	weight gain	508:518	weight gain	508:518	Low- and high-dose GOS reduced weight gain by 48.8 % and 35.3 %, and low- and high-dose SA reduced it by 37.7 % and 34.4 %, respectively.
35718141	1	10	theme	mice	331:334	arg1	metabolism					268:277	metabolism	268:277	metabolism	268:277	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	1	10	theme	mice	331:334	arg1	microbiota					287:296	gut microbiota	283:296	gut microbiota	283:296	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	3	11	theme	high-dose	486:494	arg1	GOS					496:498	Low- and high-dose GOS	477:498	Low- and high-dose GOS	477:498	Low- and high-dose GOS reduced weight gain by 48.8 % and 35.3 %, and low- and high-dose SA reduced it by 37.7 % and 34.4 %, respectively.
35718141	2	12	theme	high-fat	356:363	arg1	obesity					378:384	high-fat diet-induced obesity	356:384	high-fat diet-induced obesity	356:384	GOS and SA delayed high-fat diet-induced obesity, reduced the epididymal fat and liver indices, and improved the circulating lipid profile.
35718141	0	13	theme	microbiota	112:121	arg1	composition					89:99	the composition	85:99	the composition of the gut microbiota in mice with high-fat diet-induced obesity	85:164	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	7	14	theme	factor-α	1345:1352	arg1	concentration					1354:1366	tumor necrosis factor-α concentration	1330:1366	tumor necrosis factor-α concentration	1330:1366	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	5	15	theme	tight	766:770	arg1	occludin					800:807	occludin	800:807	occludin	800:807	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	15	theme	tight	766:770	arg1	ZO-1					791:794	ZO-1	791:794	ZO-1	791:794	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	15	theme	tight	766:770	arg1	proteins					781:788	tight junction proteins	766:788	tight junction proteins (ZO-1 and occludin)	766:808	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	7	16	theme	circulating	1193:1203	arg1	lipoprotein-cholesterol					1217:1239	the circulating low-density lipoprotein-cholesterol	1189:1239	the circulating low-density lipoprotein-cholesterol (LDL-C) concentration	1189:1261	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	7	16	theme	circulating	1193:1203	arg1	LDL-C					1242:1246	LDL-C	1242:1246	LDL-C	1242:1246	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	4	17	theme	glucose	640:646	arg1	concentration					648:660	blood glucose concentration	634:660	blood glucose concentration	634:660	GOS and SA reduced blood glucose concentration, probably by increasing the expression of glucose transporter 4.
35718141	5	18	with	consistent	934:943	arg1	function					978:985	improved intestinal barrier function	950:985	improved intestinal barrier function	950:985	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	19	theme	junction	772:779	arg1	occludin					800:807	occludin	800:807	occludin	800:807	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	19	theme	junction	772:779	arg1	ZO-1					791:794	ZO-1	791:794	ZO-1	791:794	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	19	theme	junction	772:779	arg1	proteins					781:788	tight junction proteins	766:788	tight junction proteins (ZO-1 and occludin)	766:808	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	0	20	theme	Sodium	0:5	arg1	alginate					7:14	Sodium alginate	0:14	Sodium alginate	0:14	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	4	21	theme	blood	634:638	arg1	concentration					648:660	blood glucose concentration	634:660	blood glucose concentration	634:660	GOS and SA reduced blood glucose concentration, probably by increasing the expression of glucose transporter 4.
35718141	7	22	theme	low-density	1205:1215	arg1	lipoprotein-cholesterol					1217:1239	the circulating low-density lipoprotein-cholesterol	1189:1239	the circulating low-density lipoprotein-cholesterol (LDL-C) concentration	1189:1261	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	7	22	theme	low-density	1205:1215	arg1	LDL-C					1242:1246	LDL-C	1242:1246	LDL-C	1242:1246	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	5	23	theme	barrier	970:976	arg1	function					978:985	improved intestinal barrier function	950:985	improved intestinal barrier function	950:985	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	2	24	theme	epididymal	399:408	arg1	fat					410:412	the epididymal fat	395:412	the epididymal fat	395:412	GOS and SA delayed high-fat diet-induced obesity, reduced the epididymal fat and liver indices, and improved the circulating lipid profile.
35718141	7	25	theme	tumor	1330:1334	arg1	concentration					1354:1366	tumor necrosis factor-α concentration	1330:1366	tumor necrosis factor-α concentration	1330:1366	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	7	26	theme	lipoprotein-cholesterol	1217:1239	arg1	concentration					1249:1261	the circulating low-density lipoprotein-cholesterol (LDL-C) concentration	1189:1261	the circulating low-density lipoprotein-cholesterol (LDL-C) concentration	1189:1261	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	5	27	theme	acid	832:835	arg1	concentrations					867:880	the D-lactic acid (D-LA) and lipopolysaccharide concentrations	819:880	the D-lactic acid (D-LA) and lipopolysaccharide concentrations	819:880	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	28	theme	lipopolysaccharide	848:865	arg1	concentrations					867:880	the D-lactic acid (D-LA) and lipopolysaccharide concentrations	819:880	the D-lactic acid (D-LA) and lipopolysaccharide concentrations	819:880	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	29	theme	intestinal	959:968	arg1	function					978:985	improved intestinal barrier function	950:985	improved intestinal barrier function	950:985	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	6	30	theme	Bacteroidota	1031:1042	arg1	abundance					1018:1026	the abundance	1014:1026	the abundance of Bacteroidota, Bifidobacterium, and Lactobacillus;	1014:1079	GOS and SA also increased the abundance of Bacteroidota, Bifidobacterium, and Lactobacillus; and reduced that of Patescibacteria in the gut.
35718141	1	31	theme	sodium	206:211	arg1	SA					223:224	SA	223:224	SA	223:224	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	1	31	theme	sodium	206:211	arg1	alginate					213:220	sodium alginate	206:220	sodium alginate (SA)	206:225	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	0	32	theme	metabolic	55:63	arg1	disorders					65:73	metabolic disorders	55:73	metabolic disorders	55:73	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	1	33	theme	gut	283:285	arg1	microbiota					287:296	gut microbiota	283:296	gut microbiota	283:296	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	0	34	theme	diet-induced	145:156	arg1	obesity					158:164	high-fat diet-induced obesity	136:164	high-fat diet-induced obesity	136:164	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	0	35	from	composition	89:99	arg1	mice					126:129	mice	126:129	mice with high-fat diet-induced obesity	126:164	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	5	36	theme	proteins	781:788	arg1	expression					752:761	the expression	748:761	the expression of tight junction proteins (ZO-1 and occludin)	748:808	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	1	37	theme	alginate	213:220	arg1	effects					195:201	the effects	191:201	the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice	191:334	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	0	38	theme	high-fat	136:143	arg1	obesity					158:164	high-fat diet-induced obesity	136:164	high-fat diet-induced obesity	136:164	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	5	39	dep	proteins	781:788	arg1	occludin					800:807	occludin	800:807	occludin	800:807	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	39	dep	proteins	781:788	arg1	ZO-1					791:794	ZO-1	791:794	ZO-1	791:794	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	5	39	dep	proteins	781:788	arg1	proteins					781:788	tight junction proteins	766:788	tight junction proteins (ZO-1 and occludin)	766:808	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	2	40	theme	diet-induced	365:376	arg1	obesity					378:384	high-fat diet-induced obesity	356:384	high-fat diet-induced obesity	356:384	GOS and SA delayed high-fat diet-induced obesity, reduced the epididymal fat and liver indices, and improved the circulating lipid profile.
35718141	7	41	theme	Spearman	1489:1496	arg1	analysis					1510:1517	Spearman correlation analysis	1489:1517	Spearman correlation analysis	1489:1517	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	5	42	theme	toll-like	913:921	arg1	receptors					923:931	toll-like receptors	913:931	toll-like receptors	913:931	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	8	43	from	disorders	1594:1602	arg1	mice					1607:1610	mice	1607:1610	mice	1607:1610	In conclusion, SA and GOS ameliorate obesity and the associated metabolic disorders in mice, and also modulate their gut microbial composition.
35718141	1	44	theme	-fed	320:323	arg1	mice					331:334	high-fat diet (HFD)-fed obese mice	301:334	high-fat diet (HFD)-fed obese mice	301:334	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	1	45	theme	high-fat	301:308	arg1	HFD					316:318	HFD	316:318	HFD	316:318	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	1	45	theme	high-fat	301:308	arg1	diet					310:313	high-fat diet	301:313	high-fat diet (HFD)	301:319	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	2	46	theme	liver	418:422	arg1	indices					424:430	liver indices	418:430	liver indices	418:430	GOS and SA delayed high-fat diet-induced obesity, reduced the epididymal fat and liver indices, and improved the circulating lipid profile.
35718141	5	47	theme	receptors	923:931	arg1	expression					899:908	the expression	895:908	the expression of toll-like receptors	895:931	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	4	48	theme	glucose	704:710	arg1	transporter					712:722	glucose transporter 4	704:724	glucose transporter 4	704:724	GOS and SA reduced blood glucose concentration, probably by increasing the expression of glucose transporter 4.
35718141	8	49	theme	metabolic	1584:1592	arg1	disorders					1594:1602	the associated metabolic disorders	1569:1602	the associated metabolic disorders	1569:1602	In conclusion, SA and GOS ameliorate obesity and the associated metabolic disorders in mice, and also modulate their gut microbial composition.
35718141	6	50	theme	Lactobacillus	1066:1078	arg1	abundance					1018:1026	the abundance	1014:1026	the abundance of Bacteroidota, Bifidobacterium, and Lactobacillus;	1014:1079	GOS and SA also increased the abundance of Bacteroidota, Bifidobacterium, and Lactobacillus; and reduced that of Patescibacteria in the gut.
35718141	7	51	theme	necrosis	1336:1343	arg1	concentration					1354:1366	tumor necrosis factor-α concentration	1330:1366	tumor necrosis factor-α concentration	1330:1366	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	8	52	from	obesity	1557:1563	arg1	mice					1607:1610	mice	1607:1610	mice	1607:1610	In conclusion, SA and GOS ameliorate obesity and the associated metabolic disorders in mice, and also modulate their gut microbial composition.
35718141	7	53	theme	lipoprotein-cholesterol	1437:1459	arg1	concentration					1461:1473	high-density lipoprotein-cholesterol concentration	1424:1473	high-density lipoprotein-cholesterol concentration	1424:1473	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	3	54	theme	high-dose	555:563	arg1	SA					565:566	low- and high-dose SA	546:566	low- and high-dose SA	546:566	Low- and high-dose GOS reduced weight gain by 48.8 % and 35.3 %, and low- and high-dose SA reduced it by 37.7 % and 34.4 %, respectively.
35718141	2	55	theme	lipid	462:466	arg1	profile					468:474	the circulating lipid profile	446:474	the circulating lipid profile	446:474	GOS and SA delayed high-fat diet-induced obesity, reduced the epididymal fat and liver indices, and improved the circulating lipid profile.
35718141	5	56	theme	improved	950:957	arg1	function					978:985	improved intestinal barrier function	950:985	improved intestinal barrier function	950:985	GOS and SA increased the expression of tight junction proteins (ZO-1 and occludin), reduced the D-lactic acid (D-LA) and lipopolysaccharide concentrations, and reduced the expression of toll-like receptors, consistent with improved intestinal barrier function.
35718141	4	57	theme	transporter	712:722	arg1	expression					690:699	the expression	686:699	the expression of glucose transporter 4	686:724	GOS and SA reduced blood glucose concentration, probably by increasing the expression of glucose transporter 4.
35718141	1	58	from	effects	195:201	arg1	metabolism					268:277	metabolism	268:277	metabolism	268:277	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	1	58	from	effects	195:201	arg1	microbiota					287:296	gut microbiota	283:296	gut microbiota	283:296	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35718141	3	59	theme	Low-	477:480	arg1	GOS					496:498	Low- and high-dose GOS	477:498	Low- and high-dose GOS	477:498	Low- and high-dose GOS reduced weight gain by 48.8 % and 35.3 %, and low- and high-dose SA reduced it by 37.7 % and 34.4 %, respectively.
35718141	0	60	with	mice	126:129	arg1	obesity					158:164	high-fat diet-induced obesity	136:164	high-fat diet-induced obesity	136:164	Sodium alginate and galactooligosaccharides ameliorate metabolic disorders and alter the composition of the gut microbiota in mice with high-fat diet-induced obesity.
35718141	6	61	theme	Bifidobacterium	1045:1059	arg1	abundance					1018:1026	the abundance	1014:1026	the abundance of Bacteroidota, Bifidobacterium, and Lactobacillus;	1014:1079	GOS and SA also increased the abundance of Bacteroidota, Bifidobacterium, and Lactobacillus; and reduced that of Patescibacteria in the gut.
35718141	2	62	theme	circulating	450:460	arg1	profile					468:474	the circulating lipid profile	446:474	the circulating lipid profile	446:474	GOS and SA delayed high-fat diet-induced obesity, reduced the epididymal fat and liver indices, and improved the circulating lipid profile.
35718141	3	63	theme	low-	546:549	arg1	SA					565:566	low- and high-dose SA	546:566	low- and high-dose SA	546:566	Low- and high-dose GOS reduced weight gain by 48.8 % and 35.3 %, and low- and high-dose SA reduced it by 37.7 % and 34.4 %, respectively.
35718141	7	64	theme	Parabacteroides	1146:1160	arg1	abundance					1133:1141	The abundance	1129:1141	The abundance of Parabacteroides	1129:1160	The abundance of Parabacteroides positively correlated with the circulating low-density lipoprotein-cholesterol (LDL-C) concentration; that of Lactobacillus negatively correlated with LDL-C, D-LA, and tumor necrosis factor-α concentration; and that of Bifidobacterium positively correlated with high-density lipoprotein-cholesterol concentration, according to Spearman correlation analysis.
35718141	8	65	theme	gut	1637:1639	arg1	composition					1651:1661	their gut microbial composition	1631:1661	their gut microbial composition	1631:1661	In conclusion, SA and GOS ameliorate obesity and the associated metabolic disorders in mice, and also modulate their gut microbial composition.
35718141	1	66	theme	galactooligosaccharides	231:253	arg1	effects					195:201	the effects	191:201	the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice	191:334	We aimed to investigate the effects of sodium alginate (SA) and galactooligosaccharides (GOS) on the metabolism and gut microbiota of high-fat diet (HFD)-fed obese mice.
35151255	8	0	theme	microbiota	931:940	arg1	profiling					942:950	Gut microbiota profiling	927:950	Gut microbiota profiling	927:950	Gut microbiota profiling was conducted by metagenomic high throughput sequencing technology.
35151255	14	1	dep	correlated	1985:1994	arg1	P < 0.05					1975:1982	P < 0.05	1975:1982	P < 0.05	1975:1982	In UC group, the specie Bacteroides_massiliensis was negatively (P < 0.05) correlated with IL-23, Bacteroides_eggerthii_CAG109 and Bacteroides_eggerthii were negatively (P < 0.05) correlated with TGF-β.
35151255	7	2	theme	haematoxylin	846:857	arg1	staining					869:876	haematoxylin and eosin staining	846:876	haematoxylin and eosin staining	846:876	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	10	3	theme	mesalazine	1208:1217	arg1	groups					1219:1224	the moxibustion and mesalazine groups	1188:1224	groups	1219:1224	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	2	4	dep	inflammation	231:242	arg1	the					227:229	the	227:229	the	227:229	Moxibustion has been used to improve the inflammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC.
35151255	11	5	theme	Proteobacteria	1442:1455	arg1	abundance					1417:1425	abundance	1417:1425	abundance of Firmicutes, Proteobacteria	1417:1455	Moxibustion treatment increased abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes and decreased abundance of Firmicutes, Proteobacteria.
35151255	10	6	from	scores	1152:1157	arg1	groups					1219:1224	the moxibustion and mesalazine groups	1188:1224	groups	1219:1224	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	6	7	theme	daily	783:787	arg1	mesalazine					767:776	mesalazine	767:776	mesalazine once daily for 7 consecutive days	767:810	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	11	8	dep	Synergistetes	1389:1401	arg1	Actinobacteria					1361:1374	Actinobacteria	1361:1374	Actinobacteria	1361:1374	Moxibustion treatment increased abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes and decreased abundance of Firmicutes, Proteobacteria.
35151255	8	9	theme	high	981:984	arg1	technology					1008:1017	metagenomic high throughput sequencing technology	969:1017	metagenomic high throughput sequencing technology	969:1017	Gut microbiota profiling was conducted by metagenomic high throughput sequencing technology.
35151255	7	10	theme	activity	821:828	arg1	DAI					837:839	DAI	837:839	DAI	837:839	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	7	10	theme	activity	821:828	arg1	index					830:834	Disease activity index	813:834	Disease activity index (DAI)	813:840	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	1	11	theme	gut	174:176	arg1	microbiota					178:187	the gut microbiota	170:187	the gut microbiota	170:187	BACKGROUND The pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota.
35151255	8	12	theme	sequencing	997:1006	arg1	technology					1008:1017	metagenomic high throughput sequencing technology	969:1017	metagenomic high throughput sequencing technology	969:1017	Gut microbiota profiling was conducted by metagenomic high throughput sequencing technology.
35151255	2	13	used	used	211:214	arg2	Moxibustion					190:200	Moxibustion	190:200	Moxibustion	190:200	Moxibustion has been used to improve the inflammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC.
35151255	16	14	theme	certain	2304:2310	arg1	conversion					2362:2371	conversion	2362:2371	conversion	2362:2371	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	14	theme	certain	2304:2310	arg1	pathways					2322:2329	certain metabolic pathways	2304:2329	certain metabolic pathways	2304:2329	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	14	theme	certain	2304:2310	arg1	transport					2424:2432	carbohydrate transport	2411:2432	carbohydrate transport	2411:2432	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	14	theme	certain	2304:2310	arg1	transport					2385:2393	amino acid transport	2374:2393	amino acid transport	2374:2393	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	14	theme	certain	2304:2310	arg1	metabolism					2438:2447	metabolism	2438:2447	metabolism	2438:2447	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	14	theme	certain	2304:2310	arg1	production					2347:2356	energy production	2340:2356	energy production	2340:2356	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	4	15	theme	Twenty-five	457:467	arg1	rats					474:477	METHODS Twenty-five male rats	449:477	METHODS Twenty-five male rats	449:477	METHODS Twenty-five male rats were randomly assigned into five groups.
35151255	13	16	theme	Bacteroides_bacterium_H3	1805:1828	arg1	abundance					1776:1784	the abundance	1772:1784	the abundance of Bacteroides and Bacteroides_bacterium_H3	1772:1828	Moxibustion increased the abundance of Bacteroides and Bacteroides_bacterium_H3 and decreased Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2.
35151255	5	17	theme	DSS	570:572	arg1	solution					574:581	DSS solution	570:581	DSS solution	570:581	The UC rat model was established by administering DSS solution.
35151255	1	18	theme	ulcerative	124:133	arg1	colitis					135:141	ulcerative colitis	124:141	ulcerative colitis (UC)	124:146	BACKGROUND The pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota.
35151255	1	18	theme	ulcerative	124:133	arg1	UC					144:145	UC	144:145	UC	144:145	BACKGROUND The pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota.
35151255	5	19	theme	rat	527:529	arg1	model					531:535	The UC rat model	520:535	The UC rat model	520:535	The UC rat model was established by administering DSS solution.
35151255	16	20	from	changes	2500:2506	arg1	pathways					2525:2532	the metabolic pathways	2511:2532	the metabolic pathways	2511:2532	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	9	21	theme	microbiota	1028:1037	arg1	composition					1039:1049	The gut microbiota composition	1020:1049	The gut microbiota composition	1020:1049	The gut microbiota composition, diversity and function were analyzed and compared using metagenomics methodologies.
35151255	15	22	dep	significant	2185:2195	arg1	P < 0.05					2209:2216	P < 0.05	2209:2216	P < 0.05	2209:2216	And the species Prevotella_sp_CAG1031 and Bacteroides_bacterium_H2 were significant positively (P < 0.05) correlated with IL-23.
35151255	0	23	from	alterations	15:25	arg1	rats					38:41	colitis rats	30:41	colitis rats	30:41	Gut microbiome alterations in colitis rats after moxibustion at bilateral Tianshu acupoints.
35151255	11	24	theme	Moxibustion	1294:1304	arg1	treatment					1306:1314	Moxibustion treatment	1294:1314	Moxibustion treatment	1294:1314	Moxibustion treatment increased abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes and decreased abundance of Firmicutes, Proteobacteria.
35151255	6	25	theme	moxibustion	633:643	arg1	groups					645:650	moxibustion groups	633:650	moxibustion groups	633:650	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	0	26	theme	Tianshu	74:80	arg1	acupoints					82:90	bilateral Tianshu acupoints	64:90	bilateral Tianshu acupoints	64:90	Gut microbiome alterations in colitis rats after moxibustion at bilateral Tianshu acupoints.
35151255	17	27	theme	intestinal	2728:2737	arg1	community					2750:2758	the intestinal microbiota community	2724:2758	the intestinal microbiota community	2724:2758	CONCLUSIONS Our findings suggest that moxibustion treatment may protect the host from mucosal inflammation by modulating the intestinal microbiota community.
35151255	10	28	theme	UC	1273:1274	arg1	group					1276:1280	the UC group	1269:1280	the UC group (P < 0.01)	1269:1291	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	10	28	theme	UC	1273:1274	arg1	P < 0.01					1283:1290	P < 0.01	1283:1290	P < 0.01	1283:1290	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	6	29	theme	Tianshu	685:691	arg1	points					711:716	Tianshu (bilateral, ST25) points	685:716	Tianshu (bilateral, ST25) points	685:716	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	12	30	theme	NG	1740:1741	arg1	group					1743:1747	the NG group	1736:1747	the NG group	1736:1747	At the genus level, the abundance of Bacteroides, Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2, were increased and Bacteroides_bacterium_H3, Parabacteroides, Porphyromonas, Alistipes, Parasutterella were decreased in the UC group in comparsion with those in the NG group.
35151255	3	31	theme	gut	389:391	arg1	dysbiosis					403:411	the gut microbial dysbiosis	385:411	the gut microbial dysbiosis induced by dextran sulphate sodium	385:446	In this study, we investigated whether moxibustion could improve the gut microbial dysbiosis induced by dextran sulphate sodium.
35151255	3	32	theme	sulphate	432:439	arg1	sodium					441:446	dextran sulphate sodium	424:446	dextran sulphate sodium	424:446	In this study, we investigated whether moxibustion could improve the gut microbial dysbiosis induced by dextran sulphate sodium.
35151255	10	33	theme	DAI	1148:1150	arg1	scores					1152:1157	RESULTS The DAI scores	1136:1157	RESULTS The DAI scores	1136:1157	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	2	34	from	inflammation	231:242	arg1	gastrointestinal disorders					281:306	gastrointestinal disorders	281:306	gastrointestinal disorders such as UC	281:317	Moxibustion has been used to improve the inflammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC.
35151255	2	34	from	inflammation	231:242	arg1	UC					316:317	UC	316:317	UC	316:317	Moxibustion has been used to improve the inflammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC.
35151255	10	35	theme	RESULTS	1136:1142	arg1	scores					1152:1157	RESULTS The DAI scores	1136:1157	RESULTS The DAI scores	1136:1157	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	14	36	theme	specie	1927:1932	arg1	Bacteroides_massiliensis					1934:1957	the specie Bacteroides_massiliensis	1923:1957	the specie Bacteroides_massiliensis	1923:1957	In UC group, the specie Bacteroides_massiliensis was negatively (P < 0.05) correlated with IL-23, Bacteroides_eggerthii_CAG109 and Bacteroides_eggerthii were negatively (P < 0.05) correlated with TGF-β.
35151255	0	37	theme	colitis	30:36	arg1	rats					38:41	colitis rats	30:41	colitis rats	30:41	Gut microbiome alterations in colitis rats after moxibustion at bilateral Tianshu acupoints.
35151255	7	38	used	used	883:886	arg2	staining					869:876	haematoxylin and eosin staining	846:876	haematoxylin and eosin staining	846:876	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	7	38	used	used	883:886	arg2	DAI					837:839	DAI	837:839	DAI	837:839	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	7	38	used	used	883:886	arg2	index					830:834	Disease activity index	813:834	Disease activity index (DAI)	813:840	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	16	39	theme	acid	2380:2383	arg1	transport					2385:2393	amino acid transport	2374:2393	amino acid transport	2374:2393	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	12	40	theme	Bacteroides_bacterium_M7	1508:1531	arg1	abundance					1482:1490	the abundance	1478:1490	the abundance of Bacteroides, Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2,	1478:1572	At the genus level, the abundance of Bacteroides, Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2, were increased and Bacteroides_bacterium_H3, Parabacteroides, Porphyromonas, Alistipes, Parasutterella were decreased in the UC group in comparsion with those in the NG group.
35151255	6	41	theme	group	738:742	arg1	rats					744:747	the mesalazine group rats	723:747	the mesalazine group rats	723:747	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	12	42	with	comparsion	1711:1720	arg1	those					1727:1731	those	1727:1731	those	1727:1731	At the genus level, the abundance of Bacteroides, Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2, were increased and Bacteroides_bacterium_H3, Parabacteroides, Porphyromonas, Alistipes, Parasutterella were decreased in the UC group in comparsion with those in the NG group.
35151255	8	43	theme	Gut	927:929	arg1	profiling					942:950	Gut microbiota profiling	927:950	Gut microbiota profiling	927:950	Gut microbiota profiling was conducted by metagenomic high throughput sequencing technology.
35151255	6	44	theme	normal	616:621	arg1	rats					623:626	normal rats	616:626	normal rats with moxibustion groups	616:650	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	17	45	theme	mucosal	2689:2695	arg1	inflammation					2697:2708	mucosal inflammation	2689:2708	mucosal inflammation	2689:2708	CONCLUSIONS Our findings suggest that moxibustion treatment may protect the host from mucosal inflammation by modulating the intestinal microbiota community.
35151255	16	46	theme	amino	2374:2378	arg1	transport					2385:2393	amino acid transport	2374:2393	amino acid transport	2374:2393	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	6	47	theme	consecutive	795:805	arg1	days					807:810	7 consecutive days	793:810	7 consecutive days	793:810	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	12	48	theme	genus	1465:1469	arg1	level					1471:1475	the genus level	1461:1475	the genus level	1461:1475	At the genus level, the abundance of Bacteroides, Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2, were increased and Bacteroides_bacterium_H3, Parabacteroides, Porphyromonas, Alistipes, Parasutterella were decreased in the UC group in comparsion with those in the NG group.
35151255	8	49	theme	metagenomic	969:979	arg1	technology					1008:1017	metagenomic high throughput sequencing technology	969:1017	metagenomic high throughput sequencing technology	969:1017	Gut microbiota profiling was conducted by metagenomic high throughput sequencing technology.
35151255	16	50	theme	UC	2480:2481	arg1	group					2483:2487	the UC group	2476:2487	the UC group	2476:2487	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	8	51	theme	throughput	986:995	arg1	technology					1008:1017	metagenomic high throughput sequencing technology	969:1017	metagenomic high throughput sequencing technology	969:1017	Gut microbiota profiling was conducted by metagenomic high throughput sequencing technology.
35151255	13	52	theme	Bacteroides_bacterium_M7	1844:1867	arg1	Bacteroidales_bacterium_H2					1882:1907	Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2	1844:1907	Bacteroidales_bacterium_H2	1882:1907	Moxibustion increased the abundance of Bacteroides and Bacteroides_bacterium_H3 and decreased Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2.
35151255	13	52	theme	Bacteroides_bacterium_M7	1844:1867	arg1	Prevotella					1870:1879	Prevotella	1870:1879	Prevotella	1870:1879	Moxibustion increased the abundance of Bacteroides and Bacteroides_bacterium_H3 and decreased Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2.
35151255	10	53	from	scores	1178:1183	arg1	groups					1219:1224	the moxibustion and mesalazine groups	1188:1224	groups	1219:1224	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	16	54	theme	metabolic	2515:2523	arg1	pathways					2525:2532	the metabolic pathways	2511:2532	the metabolic pathways	2511:2532	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	5	55	theme	UC	524:525	arg1	model					531:535	The UC rat model	520:535	The UC rat model	520:535	The UC rat model was established by administering DSS solution.
35151255	16	56	theme	metabolic	2312:2320	arg1	conversion					2362:2371	conversion	2362:2371	conversion	2362:2371	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	56	theme	metabolic	2312:2320	arg1	pathways					2322:2329	certain metabolic pathways	2304:2329	certain metabolic pathways	2304:2329	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	56	theme	metabolic	2312:2320	arg1	transport					2424:2432	carbohydrate transport	2411:2432	carbohydrate transport	2411:2432	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	56	theme	metabolic	2312:2320	arg1	transport					2385:2393	amino acid transport	2374:2393	amino acid transport	2374:2393	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	56	theme	metabolic	2312:2320	arg1	metabolism					2438:2447	metabolism	2438:2447	metabolism	2438:2447	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	56	theme	metabolic	2312:2320	arg1	production					2347:2356	energy production	2340:2356	energy production	2340:2356	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	6	57	with	rats	588:591	arg1	groups					645:650	moxibustion groups	633:650	moxibustion groups	633:650	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	12	58	theme	UC	1699:1700	arg1	group					1702:1706	the UC group	1695:1706	the UC group	1695:1706	At the genus level, the abundance of Bacteroides, Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2, were increased and Bacteroides_bacterium_H3, Parabacteroides, Porphyromonas, Alistipes, Parasutterella were decreased in the UC group in comparsion with those in the NG group.
35151255	4	59	theme	male	469:472	arg1	rats					474:477	METHODS Twenty-five male rats	449:477	METHODS Twenty-five male rats	449:477	METHODS Twenty-five male rats were randomly assigned into five groups.
35151255	11	60	theme	Synergistetes	1389:1401	arg1	levels					1336:1341	abundance levels	1326:1341	abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes	1326:1401	Moxibustion treatment increased abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes and decreased abundance of Firmicutes, Proteobacteria.
35151255	7	61	theme	Disease	813:819	arg1	DAI					837:839	DAI	837:839	DAI	837:839	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	7	61	theme	Disease	813:819	arg1	index					830:834	Disease activity index	813:834	Disease activity index (DAI)	813:840	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	2	62	theme	gastrointestinal	248:263	arg1	dysfunctions					265:276	gastrointestinal dysfunctions	248:276	gastrointestinal dysfunctions	248:276	Moxibustion has been used to improve the inflammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC.
35151255	1	63	theme	colitis	135:141	arg1	related					159:165	related	159:165	related	159:165	BACKGROUND The pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota.
35151255	1	63	theme	colitis	135:141	arg1	pathogenesis					108:119	The pathogenesis	104:119	The pathogenesis of ulcerative colitis (UC)	104:146	BACKGROUND The pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota.
35151255	2	64	from	dysfunctions	265:276	arg1	gastrointestinal disorders					281:306	gastrointestinal disorders	281:306	gastrointestinal disorders such as UC	281:317	Moxibustion has been used to improve the inflammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC.
35151255	2	64	from	dysfunctions	265:276	arg1	UC					316:317	UC	316:317	UC	316:317	Moxibustion has been used to improve the inflammation and gastrointestinal dysfunctions in gastrointestinal disorders such as UC.
35151255	16	65	theme	moxibustion	2555:2565	arg1	treatment					2567:2575	moxibustion treatment	2555:2575	moxibustion treatment	2555:2575	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	4	66	theme	METHODS	449:455	arg1	rats					474:477	METHODS Twenty-five male rats	449:477	METHODS Twenty-five male rats	449:477	METHODS Twenty-five male rats were randomly assigned into five groups.
35151255	6	67	from	rats	623:626	arg1	moxibustion					600:610	the moxibustion	596:610	the moxibustion	596:610	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	17	68	theme	moxibustion	2641:2651	arg1	treatment					2653:2661	moxibustion treatment	2641:2661	moxibustion treatment	2641:2661	CONCLUSIONS Our findings suggest that moxibustion treatment may protect the host from mucosal inflammation by modulating the intestinal microbiota community.
35151255	9	69	theme	gut	1024:1026	arg1	composition					1039:1049	The gut microbiota composition	1020:1049	The gut microbiota composition	1020:1049	The gut microbiota composition, diversity and function were analyzed and compared using metagenomics methodologies.
35151255	0	70	theme	bilateral	64:72	arg1	acupoints					82:90	bilateral Tianshu acupoints	64:90	bilateral Tianshu acupoints	64:90	Gut microbiome alterations in colitis rats after moxibustion at bilateral Tianshu acupoints.
35151255	1	71	dep	BACKGROUND	93:102	arg1	related					159:165	related	159:165	related	159:165	BACKGROUND The pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota.
35151255	1	71	dep	BACKGROUND	93:102	arg1	pathogenesis					108:119	The pathogenesis	104:119	The pathogenesis of ulcerative colitis (UC)	104:146	BACKGROUND The pathogenesis of ulcerative colitis (UC) is closely related to the gut microbiota.
35151255	15	72	dep	species	2121:2127	arg1	species					2121:2127	And the species Prevotella_sp_CAG1031 and Bacteroides_bacterium_H2	2113:2178	species	2121:2127	And the species Prevotella_sp_CAG1031 and Bacteroides_bacterium_H2 were significant positively (P < 0.05) correlated with IL-23.
35151255	15	72	dep	species	2121:2127	arg1	Bacteroides_bacterium_H2					2155:2178	Bacteroides_bacterium_H2	2155:2178	Bacteroides_bacterium_H2	2155:2178	And the species Prevotella_sp_CAG1031 and Bacteroides_bacterium_H2 were significant positively (P < 0.05) correlated with IL-23.
35151255	15	72	dep	species	2121:2127	arg1	Prevotella_sp_CAG1031					2129:2149	Prevotella_sp_CAG1031	2129:2149	Prevotella_sp_CAG1031	2129:2149	And the species Prevotella_sp_CAG1031 and Bacteroides_bacterium_H2 were significant positively (P < 0.05) correlated with IL-23.
35151255	6	73	theme	mesalazine	727:736	arg1	rats					744:747	the mesalazine group rats	723:747	the mesalazine group rats	723:747	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	13	74	theme	Bacteroides	1789:1799	arg1	abundance					1776:1784	the abundance	1772:1784	the abundance of Bacteroides and Bacteroides_bacterium_H3	1772:1828	Moxibustion increased the abundance of Bacteroides and Bacteroides_bacterium_H3 and decreased Bacteroides_bacterium_M7, Prevotella, Bacteroidales_bacterium_H2.
35151255	17	75	theme	microbiota	2739:2748	arg1	community					2750:2758	the intestinal microbiota community	2724:2758	the intestinal microbiota community	2724:2758	CONCLUSIONS Our findings suggest that moxibustion treatment may protect the host from mucosal inflammation by modulating the intestinal microbiota community.
35151255	6	76	with	rats	623:626	arg1	groups					645:650	moxibustion groups	633:650	moxibustion groups	633:650	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	16	77	theme	carbohydrate	2411:2422	arg1	transport					2424:2432	carbohydrate transport	2411:2432	carbohydrate transport	2411:2432	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	7	78	theme	moxibustion	914:924	arg1	effect					904:909	the effect	900:909	the effect of moxibustion	900:924	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
35151255	3	79	theme	microbial	393:401	arg1	dysbiosis					403:411	the gut microbial dysbiosis	385:411	the gut microbial dysbiosis induced by dextran sulphate sodium	385:446	In this study, we investigated whether moxibustion could improve the gut microbial dysbiosis induced by dextran sulphate sodium.
35151255	11	80	theme	abundance	1326:1334	arg1	levels					1336:1341	abundance levels	1326:1341	abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes	1326:1401	Moxibustion treatment increased abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes and decreased abundance of Firmicutes, Proteobacteria.
35151255	6	81	dep	points	711:716	arg1	ST25					705:708	ST25	705:708	ST25	705:708	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	6	81	dep	points	711:716	arg1	bilateral					694:702	bilateral	694:702	bilateral	694:702	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	9	82	theme	metagenomics	1108:1119	arg1	methodologies					1121:1133	metagenomics methodologies	1108:1133	metagenomics methodologies	1108:1133	The gut microbiota composition, diversity and function were analyzed and compared using metagenomics methodologies.
35151255	3	83	theme	dextran	424:430	arg1	sodium					441:446	dextran sulphate sodium	424:446	dextran sulphate sodium	424:446	In this study, we investigated whether moxibustion could improve the gut microbial dysbiosis induced by dextran sulphate sodium.
35151255	14	84	dep	negatively	2068:2077	arg1	P < 0.05					2080:2087	P < 0.05	2080:2087	P < 0.05	2080:2087	In UC group, the specie Bacteroides_massiliensis was negatively (P < 0.05) correlated with IL-23, Bacteroides_eggerthii_CAG109 and Bacteroides_eggerthii were negatively (P < 0.05) correlated with TGF-β.
35151255	10	85	theme	histopathology	1163:1176	arg1	scores					1178:1183	histopathology scores	1163:1183	histopathology scores	1163:1183	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	16	86	theme	energy	2340:2345	arg1	production					2347:2356	energy production	2340:2356	energy production	2340:2356	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	16	87	theme	normal	2272:2277	arg1	group					2279:2283	the normal group	2268:2283	the normal group	2268:2283	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	17	88	dep	CONCLUSIONS	2603:2613	arg1	suggest					2628:2634	suggest	2628:2634	suggest that moxibustion treatment may protect the host from mucosal inflammation by modulating the intestinal microbiota community	2628:2758	CONCLUSIONS Our findings suggest that moxibustion treatment may protect the host from mucosal inflammation by modulating the intestinal microbiota community.
35151255	16	89	theme	mesalazine	2581:2590	arg1	treatment					2592:2600	mesalazine treatment	2581:2600	mesalazine treatment	2581:2600	In addition, compare with the normal group, genes involved in certain metabolic pathways, such as energy production and conversion, amino acid transport and metabolism, carbohydrate transport and metabolism, were under-represented in the UC group, and these changes in the metabolic pathways could be reversed by moxibustion treatment and mesalazine treatment.
35151255	10	90	theme	moxibustion	1192:1202	arg1	groups					1219:1224	the moxibustion and mesalazine groups	1188:1224	groups	1219:1224	RESULTS The DAI scores and histopathology scores in the moxibustion and mesalazine groups were significantly decreased compared with the UC group (P < 0.01).
35151255	6	91	from	rats	588:591	arg1	moxibustion					600:610	the moxibustion	596:610	the moxibustion	596:610	The rats in the moxibustion and normal rats with moxibustion groups were treated with moxibustion at Tianshu (bilateral, ST25) points, and the mesalazine group rats were treated with mesalazine once daily for 7 consecutive days.
35151255	11	92	theme	Firmicutes	1430:1439	arg1	Proteobacteria					1442:1455	Firmicutes, Proteobacteria	1430:1455	Proteobacteria	1442:1455	Moxibustion treatment increased abundance levels of Bacteroidetes, Actinobacteria, Ascomycota, Synergistetes and decreased abundance of Firmicutes, Proteobacteria.
35151255	7	93	theme	eosin	863:867	arg1	staining					869:876	haematoxylin and eosin staining	846:876	haematoxylin and eosin staining	846:876	Disease activity index (DAI) and haematoxylin and eosin staining were used to evaluate the effect of moxibustion.
37276662	0	0	theme	N-linked	75:82	arg1	glycans					84:90	N-linked glycans	75:90	N-linked glycans	75:90	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	5	1	theme	monoclonal	779:788	arg1	model					806:810	a model	804:810	a model of terminal GlcNAc N-glycosylated proteins	804:853	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	5	1	theme	monoclonal	779:788	arg1	antibodies					790:799	therapeutic monoclonal antibodies	767:799	therapeutic monoclonal antibodies	767:799	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	7	2	theme	sperm	1134:1138	arg1	surface					1140:1146	the sperm surface	1130:1146	the sperm surface	1130:1146	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	7	3	gly	glycoforms	1303:1312	arg1	mannose					1295:1301	high mannose glycoforms	1290:1312	high mannose glycoforms	1290:1312	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	4	4	link	N-linked	737:744	arg1	glycosylation					746:758	terminal GlcNAc N-linked glycosylation	721:758	terminal GlcNAc N-linked glycosylation	721:758	We speculated that AANL6 could also be used to identify terminal GlcNAc N-linked glycosylation.
37276662	6	5	theme	human	1038:1042	arg1	serum					1044:1048	human serum	1038:1048	human serum	1038:1048	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
37276662	8	6	theme	glycosylation	1476:1488	arg1	function					1459:1466	the function	1455:1466	the function of this glycosylation	1455:1488	In conclusion, AANL6 was identified as a powerful tool to probe terminal GlcNAc N-linked glycosylation and would be valuable for uncovering the function of this glycosylation.
37276662	1	7	gly	N-glycosylated	169:182	arg1	modification					184:195	a truncated N-glycosylated modification	157:195	a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases	157:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	7	gly	N-glycosylated	169:182	arg1	glycosylation					140:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	8	theme	truncated	159:167	arg1	modification					184:195	a truncated N-glycosylated modification	157:195	a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases	157:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	8	theme	truncated	159:167	arg1	glycosylation					140:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	3	9	link	O-linked	641:648	arg1	glycosylation					650:662	terminal GlcNAc O-linked glycosylation	625:662	terminal GlcNAc O-linked glycosylation	625:662	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	7	10	theme	terminal	1174:1181	arg1	N-glycans					1190:1198	abundant terminal GlcNAc N-glycans	1165:1198	abundant terminal GlcNAc N-glycans	1165:1198	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	8	11	theme	N-linked	1395:1402	arg1	glycosylation					1404:1416	terminal GlcNAc N-linked glycosylation	1379:1416	terminal GlcNAc N-linked glycosylation	1379:1416	In conclusion, AANL6 was identified as a powerful tool to probe terminal GlcNAc N-linked glycosylation and would be valuable for uncovering the function of this glycosylation.
37276662	8	12	theme	powerful	1356:1363	arg1	AANL6					1330:1334	AANL6	1330:1334	AANL6	1330:1334	In conclusion, AANL6 was identified as a powerful tool to probe terminal GlcNAc N-linked glycosylation and would be valuable for uncovering the function of this glycosylation.
37276662	8	12	theme	powerful	1356:1363	arg1	tool					1365:1368	a powerful tool	1354:1368	a powerful tool to probe terminal GlcNAc N-linked glycosylation	1354:1416	In conclusion, AANL6 was identified as a powerful tool to probe terminal GlcNAc N-linked glycosylation and would be valuable for uncovering the function of this glycosylation.
37276662	0	13	theme	glycans	84:90	arg1	N-acetylglucosamine					52:70	non-reducing N-acetylglucosamine	39:70	non-reducing N-acetylglucosamine of N-linked glycans	39:90	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	4	14	used	used	704:707	arg2	AANL6					684:688	AANL6	684:688	AANL6	684:688	We speculated that AANL6 could also be used to identify terminal GlcNAc N-linked glycosylation.
37276662	5	15	theme	N-linked	920:927	arg1	glycosylation					929:941	terminal GlcNAc N-linked glycosylation	904:941	terminal GlcNAc N-linked glycosylation	904:941	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	1	16	theme	N-linked	131:138	arg1	modification					184:195	a truncated N-glycosylated modification	157:195	a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases	157:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	16	theme	N-linked	131:138	arg1	glycosylation					140:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	7	17	theme	GlcNAc	1183:1188	arg1	N-glycans					1190:1198	abundant terminal GlcNAc N-glycans	1165:1198	abundant terminal GlcNAc N-glycans	1165:1198	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	7	18	theme	mannose	1295:1301	arg1	glycoforms					1303:1312	high mannose glycoforms	1290:1312	high mannose glycoforms	1290:1312	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	3	19	theme	GlcNAc	530:535	arg1	lectin					547:552	GlcNAc selective lectin	530:552	an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL)	479:559	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	3	19	theme	GlcNAc	530:535	arg1	AANL					555:558	AANL	555:558	AANL	555:558	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	1	20	theme	N-glycosylated	169:182	arg1	modification					184:195	a truncated N-glycosylated modification	157:195	a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases	157:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	20	theme	N-glycosylated	169:182	arg1	glycosylation					140:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	21	theme	autoimmune	264:273	arg1	diseases					275:282	autoimmune diseases	264:282	autoimmune diseases	264:282	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	3	22	theme	selective	537:545	arg1	lectin					547:552	GlcNAc selective lectin	530:552	an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL)	479:559	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	3	22	theme	selective	537:545	arg1	AANL					555:558	AANL	555:558	AANL	555:558	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	5	23	theme	terminal	815:822	arg1	proteins					846:853	terminal GlcNAc N-glycosylated proteins	815:853	terminal GlcNAc N-glycosylated proteins	815:853	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	4	24	theme	N-linked	737:744	arg1	glycosylation					746:758	terminal GlcNAc N-linked glycosylation	721:758	terminal GlcNAc N-linked glycosylation	721:758	We speculated that AANL6 could also be used to identify terminal GlcNAc N-linked glycosylation.
37276662	2	25	theme	further	375:381	arg1	characterization					383:398	further characterization	375:398	further characterization of the functions of this kind of glycosylation	375:445	New and simple tools will be always valuable for further characterization of the functions of this kind of glycosylation.
37276662	2	26	theme	glycosylation	433:445	arg1	kind					425:428	this kind	420:428	this kind of glycosylation	420:445	New and simple tools will be always valuable for further characterization of the functions of this kind of glycosylation.
37276662	6	27	theme	terminal	957:964	arg1	glycosylation					982:994	terminal GlcNAc N-linked glycosylation	957:994	terminal GlcNAc N-linked glycosylation	957:994	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
37276662	4	28	theme	GlcNAc	730:735	arg1	glycosylation					746:758	terminal GlcNAc N-linked glycosylation	721:758	terminal GlcNAc N-linked glycosylation	721:758	We speculated that AANL6 could also be used to identify terminal GlcNAc N-linked glycosylation.
37276662	0	29	theme	new	11:13	arg1	tool					25:28	a new efficient tool	9:28	a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans	9:90	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	0	29	theme	new	11:13	arg1	AANL6					0:4	AANL6	0:4	AANL6	0:4	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	5	30	theme	GlcNAc	824:829	arg1	proteins					846:853	terminal GlcNAc N-glycosylated proteins	815:853	terminal GlcNAc N-glycosylated proteins	815:853	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	5	31	link	N-linked	920:927	arg1	glycosylation					929:941	terminal GlcNAc N-linked glycosylation	904:941	terminal GlcNAc N-linked glycosylation	904:941	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	8	32	theme	terminal	1379:1386	arg1	glycosylation					1404:1416	terminal GlcNAc N-linked glycosylation	1379:1416	terminal GlcNAc N-linked glycosylation	1379:1416	In conclusion, AANL6 was identified as a powerful tool to probe terminal GlcNAc N-linked glycosylation and would be valuable for uncovering the function of this glycosylation.
37276662	1	33	theme	Terminal	93:100	arg1	GlcNAc					123:128	GlcNAc	123:128	GlcNAc	123:128	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	33	theme	Terminal	93:100	arg1	N-acetylglucosamine					102:120	Terminal N-acetylglucosamine	93:120	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	7	34	theme	high	1290:1293	arg1	glycoforms					1303:1312	high mannose glycoforms	1290:1312	high mannose glycoforms	1290:1312	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	2	35	theme	kind	425:428	arg1	functions					407:415	the functions	403:415	the functions of this kind of glycosylation	403:445	New and simple tools will be always valuable for further characterization of the functions of this kind of glycosylation.
37276662	7	36	theme	complex	1085:1091	arg1	sample					1093:1098	a complex sample	1083:1098	a complex sample	1083:1098	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	7	36	theme	complex	1085:1091	arg1	proteins					1071:1078	cell membrane proteins	1057:1078	cell membrane proteins	1057:1078	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	6	37	with	enrichment	1013:1022	arg1	AANL6					1029:1033	AANL6	1029:1033	AANL6	1029:1033	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
37276662	3	38	theme	O-linked	641:648	arg1	glycosylation					650:662	terminal GlcNAc O-linked glycosylation	625:662	terminal GlcNAc O-linked glycosylation	625:662	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	1	39	theme	N-acetylglucosamine	102:120	arg1	modification					184:195	a truncated N-glycosylated modification	157:195	a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases	157:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	39	theme	N-acetylglucosamine	102:120	arg1	glycosylation					140:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	8	40	link	N-linked	1395:1402	arg1	glycosylation					1404:1416	terminal GlcNAc N-linked glycosylation	1379:1416	terminal GlcNAc N-linked glycosylation	1379:1416	In conclusion, AANL6 was identified as a powerful tool to probe terminal GlcNAc N-linked glycosylation and would be valuable for uncovering the function of this glycosylation.
37276662	5	41	theme	terminal	904:911	arg1	glycosylation					929:941	terminal GlcNAc N-linked glycosylation	904:941	terminal GlcNAc N-linked glycosylation	904:941	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	6	42	from	enrichment	1013:1022	arg1	serum					1044:1048	human serum	1038:1048	human serum	1038:1048	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
37276662	2	43	theme	functions	407:415	arg1	characterization					383:398	further characterization	375:398	further characterization of the functions of this kind of glycosylation	375:445	New and simple tools will be always valuable for further characterization of the functions of this kind of glycosylation.
37276662	0	44	theme	efficient	15:23	arg1	tool					25:28	a new efficient tool	9:28	a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans	9:90	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	0	44	theme	efficient	15:23	arg1	AANL6					0:4	AANL6	0:4	AANL6	0:4	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	6	45	theme	glycosylation	982:994	arg1	ratio					948:952	The ratio	944:952	The ratio of terminal GlcNAc N-linked glycosylation	944:994	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
37276662	7	46	theme	abundant	1165:1172	arg1	N-glycans					1190:1198	abundant terminal GlcNAc N-glycans	1165:1198	abundant terminal GlcNAc N-glycans	1165:1198	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	3	47	theme	terminal	625:632	arg1	glycosylation					650:662	terminal GlcNAc O-linked glycosylation	625:662	terminal GlcNAc O-linked glycosylation	625:662	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	5	48	theme	N-glycosylated	831:844	arg1	proteins					846:853	terminal GlcNAc N-glycosylated proteins	815:853	terminal GlcNAc N-glycosylated proteins	815:853	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	0	49	link	N-linked	75:82	arg1	glycans					84:90	N-linked glycans	75:90	N-linked glycans	75:90	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	3	50	theme	previous	452:459	arg1	paper					461:465	Our previous paper	448:465	Our previous paper	448:465	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	1	51	theme	neurodegenerative	298:314	arg1	diseases					316:323	neurodegenerative diseases	298:323	neurodegenerative diseases	298:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	3	52	theme	GlcNAc	634:639	arg1	glycosylation					650:662	terminal GlcNAc O-linked glycosylation	625:662	terminal GlcNAc O-linked glycosylation	625:662	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	8	53	theme	GlcNAc	1388:1393	arg1	glycosylation					1404:1416	terminal GlcNAc N-linked glycosylation	1379:1416	terminal GlcNAc N-linked glycosylation	1379:1416	In conclusion, AANL6 was identified as a powerful tool to probe terminal GlcNAc N-linked glycosylation and would be valuable for uncovering the function of this glycosylation.
37276662	7	54	theme	cell	1057:1060	arg1	sample					1093:1098	a complex sample	1083:1098	a complex sample	1083:1098	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	7	54	theme	cell	1057:1060	arg1	proteins					1071:1078	cell membrane proteins	1057:1078	cell membrane proteins	1057:1078	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	3	55	theme	optimized	482:490	arg1	lectin					492:497	an optimized lectin	479:497	an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL)	479:559	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	6	56	theme	GlcNAc	966:971	arg1	glycosylation					982:994	terminal GlcNAc N-linked glycosylation	957:994	terminal GlcNAc N-linked glycosylation	957:994	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
37276662	1	57	link	N-linked	131:138	arg1	modification					184:195	a truncated N-glycosylated modification	157:195	a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases	157:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	57	link	N-linked	131:138	arg1	glycosylation					140:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation	93:152	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	5	58	gly	N-glycosylated	831:844	arg1	proteins					846:853	terminal GlcNAc N-glycosylated proteins	815:853	terminal GlcNAc N-glycosylated proteins	815:853	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	2	59	theme	simple	334:339	arg1	tools					341:345	New and simple tools	326:345	New and simple tools	326:345	New and simple tools will be always valuable for further characterization of the functions of this kind of glycosylation.
37276662	6	60	link	N-linked	973:980	arg1	glycosylation					982:994	terminal GlcNAc N-linked glycosylation	957:994	terminal GlcNAc N-linked glycosylation	957:994	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
37276662	7	61	theme	membrane	1062:1069	arg1	sample					1093:1098	a complex sample	1083:1098	a complex sample	1083:1098	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	7	61	theme	membrane	1062:1069	arg1	proteins					1071:1078	cell membrane proteins	1057:1078	cell membrane proteins	1057:1078	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	5	62	theme	therapeutic	767:777	arg1	model					806:810	a model	804:810	a model of terminal GlcNAc N-glycosylated proteins	804:853	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	5	62	theme	therapeutic	767:777	arg1	antibodies					790:799	therapeutic monoclonal antibodies	767:799	therapeutic monoclonal antibodies	767:799	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	4	63	theme	terminal	721:728	arg1	glycosylation					746:758	terminal GlcNAc N-linked glycosylation	721:758	terminal GlcNAc N-linked glycosylation	721:758	We speculated that AANL6 could also be used to identify terminal GlcNAc N-linked glycosylation.
37276662	3	64	dep	lectin	492:497	arg1	lectin					547:552	GlcNAc selective lectin	530:552	an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL)	479:559	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	3	64	dep	lectin	492:497	arg1	AANL					555:558	AANL	555:558	AANL	555:558	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	7	65	from	rich	1282:1285	arg1	glycoforms					1303:1312	high mannose glycoforms	1290:1312	high mannose glycoforms	1290:1312	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	7	66	from	glycoforms	1303:1312	arg1	rich					1282:1285	rich	1282:1285	rich	1282:1285	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	2	67	theme	New	326:328	arg1	tools					341:345	New and simple tools	326:345	New and simple tools	326:345	New and simple tools will be always valuable for further characterization of the functions of this kind of glycosylation.
37276662	7	68	theme	tumor	1226:1230	arg1	surfaces					1237:1244	some tumor cell surfaces	1221:1244	some tumor cell surfaces	1221:1244	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	3	69	theme	Agrocybe	512:519	arg1	aegerita					521:528	Agrocybe aegerita	512:528	Agrocybe aegerita	512:528	Our previous paper proved that an optimized lectin created from Agrocybe aegerita GlcNAc selective lectin (AANL) named AANL6, can effectively identify O-GlcNAcylation, which is terminal GlcNAc O-linked glycosylation.
37276662	7	70	theme	cell	1232:1235	arg1	surfaces					1237:1244	some tumor cell surfaces	1221:1244	some tumor cell surfaces	1221:1244	Using cell membrane proteins as a complex sample, we found that AANL6 bound to the sperm surface, which expresses abundant terminal GlcNAc N-glycans, but did not bind to some tumor cell surfaces such A549 and MCF-7 cells, which is rich in high mannose glycoforms.
37276662	1	71	theme	various	238:244	arg1	cancers					285:291	cancers	285:291	cancers	285:291	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	71	theme	various	238:244	arg1	diseases					275:282	autoimmune diseases	264:282	autoimmune diseases	264:282	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	71	theme	various	238:244	arg1	diseases					246:253	various diseases	238:253	various diseases	238:253	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	1	71	theme	various	238:244	arg1	diseases					316:323	neurodegenerative diseases	298:323	neurodegenerative diseases	298:323	Terminal N-acetylglucosamine (GlcNAc) N-linked glycosylation is a truncated N-glycosylated modification that has been reported to be involved in various diseases, such as autoimmune diseases, cancers, and neurodegenerative diseases.
37276662	0	72	theme	non-reducing	39:50	arg1	N-acetylglucosamine					52:70	non-reducing N-acetylglucosamine	39:70	non-reducing N-acetylglucosamine of N-linked glycans	39:90	AANL6 is a new efficient tool to probe non-reducing N-acetylglucosamine of N-linked glycans.
37276662	5	73	theme	proteins	846:853	arg1	model					806:810	a model	804:810	a model of terminal GlcNAc N-glycosylated proteins	804:853	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	5	73	theme	proteins	846:853	arg1	antibodies					790:799	therapeutic monoclonal antibodies	767:799	therapeutic monoclonal antibodies	767:799	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	5	74	theme	GlcNAc	913:918	arg1	glycosylation					929:941	terminal GlcNAc N-linked glycosylation	904:941	terminal GlcNAc N-linked glycosylation	904:941	Using therapeutic monoclonal antibodies as a model of terminal GlcNAc N-glycosylated proteins, we proved that AANL6 could selectively identify terminal GlcNAc N-linked glycosylation.
37276662	6	75	theme	N-linked	973:980	arg1	glycosylation					982:994	terminal GlcNAc N-linked glycosylation	957:994	terminal GlcNAc N-linked glycosylation	957:994	The ratio of terminal GlcNAc N-linked glycosylation was increased by enrichment with AANL6 in human serum.
36640899	3	0	dep	METHODS	322:328	arg1	used					333:336	used	333:336	used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila	333:491	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	9	1	theme	carbohydrates	1495:1507	arg1	balance					1472:1478	the dietary balance	1460:1478	the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine	1460:1592	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	3	2	dep	organism	473:480	arg1	Drosophila					482:491	the model organism Drosophila	463:491	the model organism Drosophila	463:491	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	2	3	from	investigation	246:258	arg1	effect					267:272	the effect	263:272	the effect of dietary macronutrient balance on sleep	263:314	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	7	4	theme	threonine	1244:1252	arg1	pathway					1265:1271	the glycine, serine, and threonine metabolism pathway	1219:1271	pathway	1265:1271	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	8	5	theme	glycine	1350:1356	arg1	contents					1338:1345	the contents	1334:1345	the contents of glycine, serine, and threonine	1334:1379	Further studies confirmed that the contents of glycine, serine, and threonine affected sleep.
36640899	2	6	from	effect	267:272	arg1	sleep					310:314	sleep	310:314	sleep	310:314	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	6	7	theme	hemolymph	981:989	arg1	glucose					991:997	hemolymph glucose	981:997	hemolymph glucose	981:997	We further found that the effects of macronutrients on sleep mirrored levels of hemolymph glucose and whole-body glycogen.
36640899	5	8	theme	sleep	885:889	arg1	duration					891:898	longer sleep duration	878:898	longer sleep duration	878:898	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	5	9	dep	FINDINGS	714:721	arg1	showed					735:740	showed	735:740	showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration	735:898	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	8	10	theme	Further	1303:1309	arg1	studies					1311:1317	Further studies	1303:1317	Further studies	1303:1317	Further studies confirmed that the contents of glycine, serine, and threonine affected sleep.
36640899	2	11	theme	systematic	235:244	arg1	investigation					246:258	a systematic investigation	233:258	a systematic investigation on the effect of dietary macronutrient balance on sleep	233:314	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	3	12	theme	MAIN	317:320	arg1	METHODS					322:328	MAIN METHODS	317:328	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.	317:492	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	5	13	theme	sleep	859:863	arg1	episodes					865:872	fewer sleep episodes	853:872	fewer sleep episodes	853:872	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	4	14	theme	Activity	679:686	arg1	system					702:707	the Drosophila Activity Monitor (DAM) system	664:707	the Drosophila Activity Monitor (DAM) system	664:707	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	4	15	theme	female	499:504	arg1	flies					515:519	Both female and male flies	494:519	Both female and male flies	494:519	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	3	16	theme	protein	426:432	arg1	sleep					417:421	sleep	417:421	sleep of protein (P) and carbohydrates (C)	417:458	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	1	17	theme	fundamental	57:67	arg1	function					83:90	a fundamental physiological function	55:90	a fundamental physiological function	55:90	AIMS Sleep is a fundamental physiological function and is essential for all animals.
36640899	1	17	theme	fundamental	57:67	arg1	Sleep					46:50	AIMS Sleep	41:50	AIMS Sleep	41:50	AIMS Sleep is a fundamental physiological function and is essential for all animals.
36640899	9	18	from	change	1534:1539	arg1	threonine					1584:1592	threonine	1584:1592	threonine	1584:1592	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	9	18	from	change	1534:1539	arg1	glycine					1563:1569	glycine	1563:1569	glycine	1563:1569	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	9	18	from	change	1534:1539	arg1	glycogen					1553:1560	glycogen	1553:1560	glycogen	1553:1560	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	9	18	from	change	1534:1539	arg1	glucose					1544:1550	glucose	1544:1550	glucose	1544:1550	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	9	18	from	change	1534:1539	arg1	serine					1572:1577	serine	1572:1577	serine	1572:1577	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	5	19	theme	low	766:768	arg1	protein					770:776	low protein	766:776	low protein	766:776	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	5	20	theme	KEY	710:712	arg1	FINDINGS					714:721	KEY FINDINGS	710:721	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.	710:899	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	6	21	theme	glycogen	1014:1021	arg1	levels					971:976	levels	971:976	levels of hemolymph glucose and whole-body glycogen	971:1021	We further found that the effects of macronutrients on sleep mirrored levels of hemolymph glucose and whole-body glycogen.
36640899	6	22	from	effects	927:933	arg1	sleep					956:960	sleep	956:960	sleep	956:960	We further found that the effects of macronutrients on sleep mirrored levels of hemolymph glucose and whole-body glycogen.
36640899	1	23	theme	physiological	69:81	arg1	function					83:90	a fundamental physiological function	55:90	a fundamental physiological function	55:90	AIMS Sleep is a fundamental physiological function and is essential for all animals.
36640899	1	23	theme	physiological	69:81	arg1	Sleep					46:50	AIMS Sleep	41:50	AIMS Sleep	41:50	AIMS Sleep is a fundamental physiological function and is essential for all animals.
36640899	5	24	theme	protein	770:776	arg1	combination					751:761	the combination	747:761	the combination of low protein and high carbohydrates (LPHC)	747:806	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	0	25	theme	geometry	12:19	arg1	framework					21:29	Nutritional geometry framework	0:29	Nutritional geometry framework of sleep	0:38	Nutritional geometry framework of sleep.
36640899	5	26	theme	sleep	833:837	arg1	quality					839:845	sleep quality	833:845	sleep quality	833:845	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	7	27	theme	high-protein	1074:1085	arg1	diet					1112:1115	a high-protein, low-carbohydrate (HPLC) diet	1072:1115	a high-protein, low-carbohydrate (HPLC) diet	1072:1115	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	0	28	theme	Nutritional	0:10	arg1	framework					21:29	Nutritional geometry framework	0:29	Nutritional geometry framework of sleep	0:38	Nutritional geometry framework of sleep.
36640899	9	29	theme	dietary	1464:1470	arg1	balance					1472:1478	the dietary balance	1460:1478	the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine	1460:1592	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	4	30	theme	protein-to-carbohydrates	571:594	arg1	ratios					561:566	seven ratios	555:566	seven ratios of protein-to-carbohydrates	555:594	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	4	31	theme	Drosophila	668:677	arg1	system					702:707	the Drosophila Activity Monitor (DAM) system	664:707	the Drosophila Activity Monitor (DAM) system	664:707	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	9	32	theme	protein	1483:1489	arg1	balance					1472:1478	the dietary balance	1460:1478	the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine	1460:1592	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	5	33	theme	fewer	853:857	arg1	episodes					865:872	fewer sleep episodes	853:872	fewer sleep episodes	853:872	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	6	34	theme	whole-body	1003:1012	arg1	glycogen					1014:1021	whole-body glycogen	1003:1021	whole-body glycogen	1003:1021	We further found that the effects of macronutrients on sleep mirrored levels of hemolymph glucose and whole-body glycogen.
36640899	4	35	theme	DAM	697:699	arg1	system					702:707	the Drosophila Activity Monitor (DAM) system	664:707	the Drosophila Activity Monitor (DAM) system	664:707	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	4	36	contain	containing	544:553	arg1	diets					538:542	various diets	530:542	various diets containing seven ratios of protein-to-carbohydrates at different energetic levels	530:624	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	4	36	contain	containing	544:553	arg2	ratios					561:566	seven ratios	555:566	seven ratios of protein-to-carbohydrates	555:594	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	3	37	theme	model	467:471	arg1	organism					473:480	the model organism Drosophila	463:491	the model organism Drosophila	463:491	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	4	38	theme	various	530:536	arg1	diets					538:542	various diets	530:542	various diets containing seven ratios of protein-to-carbohydrates at different energetic levels	530:624	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	7	39	theme	LPHC	1203:1206	arg1	diet					1208:1211	the LPHC diet	1199:1211	the LPHC diet	1199:1211	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	5	40	theme	sleep	818:822	arg1	time					824:827	sleep time	818:827	sleep time	818:827	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	9	41	dep	SIGNIFICANCE	1397:1408	arg1	demonstrate					1422:1432	demonstrate	1422:1432	demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine	1422:1592	SIGNIFICANCE Our results demonstrate that sleep is affected by the dietary balance of protein and carbohydrates possibly mediated by the change in glucose, glycogen, glycine, serine, and threonine.
36640899	7	42	dep	high-protein	1074:1085	arg1	low-carbohydrate					1088:1103	low-carbohydrate	1088:1103	low-carbohydrate	1088:1103	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	5	43	theme	high	782:785	arg1	LPHC					802:805	LPHC	802:805	LPHC	802:805	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	5	43	theme	high	782:785	arg1	carbohydrates					787:799	high carbohydrates	782:799	high carbohydrates (LPHC)	782:806	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	0	44	theme	sleep	34:38	arg1	framework					21:29	Nutritional geometry framework	0:29	Nutritional geometry framework of sleep	0:38	Nutritional geometry framework of sleep.
36640899	3	45	used	used	333:336	arg2	We					330:331	We	330:331	We	330:331	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	7	46	theme	glycine	1223:1229	arg1	serine					1232:1237	the glycine, serine, and threonine metabolism pathway	1219:1271	serine	1232:1237	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	3	47	theme	interactive	394:404	arg1	effects					406:412	the interactive effects	390:412	the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila	390:491	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	3	48	theme	nutritional	342:352	arg1	NGF					374:376	NGF	374:376	NGF	374:376	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	3	48	theme	nutritional	342:352	arg1	framework					363:371	the nutritional geometry framework	338:371	the nutritional geometry framework (NGF)	338:377	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	5	49	theme	carbohydrates	787:799	arg1	combination					751:761	the combination	747:761	the combination of low protein and high carbohydrates (LPHC)	747:806	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	7	50	dep	low-carbohydrate	1088:1103	arg1	HPLC					1106:1109	HPLC	1106:1109	HPLC	1106:1109	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	2	51	theme	diet	147:150	arg1	carbohydrates					191:203	carbohydrates	191:203	carbohydrates (C)	191:207	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	2	51	theme	diet	147:150	arg1	protein					175:181	protein (P) and carbohydrates (C)	175:207	protein	175:181	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	2	51	theme	diet	147:150	arg1	compositions					152:163	diet compositions	147:163	diet compositions including protein (P) and carbohydrates (C)	147:207	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	7	52	theme	metabolism	1254:1263	arg1	pathway					1265:1271	the glycine, serine, and threonine metabolism pathway	1219:1271	pathway	1265:1271	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	2	53	theme	balance	299:305	arg1	effect					267:272	the effect	263:272	the effect of dietary macronutrient balance on sleep	263:314	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	3	54	theme	geometry	354:361	arg1	NGF					374:376	NGF	374:376	NGF	374:376	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	3	54	theme	geometry	354:361	arg1	framework					363:371	the nutritional geometry framework	338:371	the nutritional geometry framework (NGF)	338:377	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	3	55	from	effects	406:412	arg1	sleep					417:421	sleep	417:421	sleep of protein (P) and carbohydrates (C)	417:458	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	3	55	from	effects	406:412	arg1	organism					473:480	the model organism Drosophila	463:491	the model organism Drosophila	463:491	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	2	56	theme	macronutrient	285:297	arg1	balance					299:305	dietary macronutrient balance	277:305	dietary macronutrient balance	277:305	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	7	57	theme	metabolic	1163:1171	arg1	pathways					1173:1180	metabolic pathways	1163:1180	metabolic pathways	1163:1180	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	7	58	theme	transcriptomic	1034:1047	arg1	analyses					1049:1056	transcriptomic analyses	1034:1056	transcriptomic analyses	1034:1056	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	8	59	theme	serine	1359:1364	arg1	contents					1338:1345	the contents	1334:1345	the contents of glycine, serine, and threonine	1334:1379	Further studies confirmed that the contents of glycine, serine, and threonine affected sleep.
36640899	5	60	theme	longer	878:883	arg1	duration					891:898	longer sleep duration	878:898	longer sleep duration	878:898	KEY FINDINGS Our results showed that the combination of low protein and high carbohydrates (LPHC) prolonged sleep time and sleep quality, with fewer sleep episodes and longer sleep duration.
36640899	2	61	theme	dietary	277:283	arg1	balance					299:305	dietary macronutrient balance	277:305	dietary macronutrient balance	277:305	Sleep is affected by diet compositions including protein (P) and carbohydrates (C), but there has not been a systematic investigation on the effect of dietary macronutrient balance on sleep.
36640899	8	62	theme	threonine	1371:1379	arg1	contents					1338:1345	the contents	1334:1345	the contents of glycine, serine, and threonine	1334:1379	Further studies confirmed that the contents of glycine, serine, and threonine affected sleep.
36640899	4	63	theme	Monitor	688:694	arg1	system					702:707	the Drosophila Activity Monitor (DAM) system	664:707	the Drosophila Activity Monitor (DAM) system	664:707	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	4	64	theme	different	599:607	arg1	levels					619:624	different energetic levels	599:624	different energetic levels	599:624	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	4	65	theme	male	510:513	arg1	flies					515:519	Both female and male flies	494:519	Both female and male flies	494:519	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	6	66	theme	glucose	991:997	arg1	levels					971:976	levels	971:976	levels of hemolymph glucose and whole-body glycogen	971:1021	We further found that the effects of macronutrients on sleep mirrored levels of hemolymph glucose and whole-body glycogen.
36640899	1	67	theme	AIMS	41:44	arg1	function					83:90	a fundamental physiological function	55:90	a fundamental physiological function	55:90	AIMS Sleep is a fundamental physiological function and is essential for all animals.
36640899	1	67	theme	AIMS	41:44	arg1	Sleep					46:50	AIMS Sleep	41:50	AIMS Sleep	41:50	AIMS Sleep is a fundamental physiological function and is essential for all animals.
36640899	6	68	theme	macronutrients	938:951	arg1	effects					927:933	the effects	923:933	the effects of macronutrients on sleep	923:960	We further found that the effects of macronutrients on sleep mirrored levels of hemolymph glucose and whole-body glycogen.
36640899	4	69	theme	energetic	609:617	arg1	levels					619:624	different energetic levels	599:624	different energetic levels	599:624	Both female and male flies were fed various diets containing seven ratios of protein-to-carbohydrates at different energetic levels for 5 days and sleep was monitored by the Drosophila Activity Monitor (DAM) system.
36640899	7	70	theme	pathways	1173:1180	arg1	expression					1149:1158	the gene expression	1140:1158	the gene expression of metabolic pathways	1140:1180	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36640899	3	71	theme	carbohydrates	442:454	arg1	sleep					417:421	sleep	417:421	sleep of protein (P) and carbohydrates (C)	417:458	MAIN METHODS We used the nutritional geometry framework (NGF) to explore the interactive effects on sleep of protein (P) and carbohydrates (C) in the model organism Drosophila.
36640899	7	72	theme	gene	1144:1147	arg1	expression					1149:1158	the gene expression	1140:1158	the gene expression of metabolic pathways	1140:1180	Moreover, transcriptomic analyses revealed that a high-protein, low-carbohydrate (HPLC) diet significantly elevated the gene expression of metabolic pathways when compared to the LPHC diet, with the glycine, serine, and threonine metabolism pathway being most strongly elevated.
36067066	1	0	theme	present	142:148	arg1	study					150:154	The present study	138:154	The present study	138:154	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	4	1	theme	differential	647:658	arg1	calorimetry					669:679	differential scanning calorimetry	647:679	differential scanning calorimetry	647:679	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	3	2	theme	BA	537:538	arg1	NPs					544:546	the BA LCH NPs	533:546	the BA LCH NPs	533:546	Then, the BA LCH NPs were characterized based on morphology, particle size, and zeta potentials.
36067066	1	3	theme	low-molecular-weight	203:222	arg1	mPEG-PLGA					314:322	mPEG-PLGA	314:322	mPEG-PLGA	314:322	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	3	theme	low-molecular-weight	203:222	arg1	poly					257:260	low-molecular-weight chitosan-coated baicalin methoxy poly	203:260	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	3	theme	low-molecular-weight	203:222	arg1	glycol					271:276	ethylene glycol	262:276	ethylene glycol	262:276	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	4	4	theme	calorimetry	669:679	arg1	data					639:642	The analytical data	624:642	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy	624:736	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	11	5	theme	BA	1727:1728	arg1	NPs					1734:1736	BA LCH NPs	1727:1736	BA LCH NPs	1727:1736	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	7	6	theme	LCH	1058:1060	arg1	NPs					1062:1064	BA LCH NPs	1055:1064	BA LCH NPs	1055:1064	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	9	7	theme	BA	1384:1385	arg1	NPs					1391:1393	The optimized BA LCH NPs	1370:1393	The optimized BA LCH NPs	1370:1393	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	6	8	theme	selenium	958:965	arg1	model					976:980	the chronic selenium cataract model	946:980	the chronic selenium cataract model	946:980	In vivo, the chronic selenium cataract model was selected to assess the therapeutic effect of BA LCH NPs.
36067066	12	9	theme	LCH	2002:2004	arg1	NPs					2006:2008	the BA LCH NPs	1995:2008	the BA LCH NPs	1995:2008	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	12	9	theme	LCH	2002:2004	arg1	system					2040:2045	a promising drug delivery system	2014:2045	a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms	2014:2163	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	11	10	theme	solution	1949:1956	arg1	group					1958:1962	the BA solution group	1942:1962	the BA solution group	1942:1962	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	5	11	theme	potential	837:845	arg1	irritation					854:863	potential ocular irritation	837:863	potential ocular irritation	837:863	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	8	12	theme	uptake	1152:1157	arg1	results					1159:1165	Cellular uptake results	1143:1165	Cellular uptake results	1143:1165	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	11	13	theme	catalase	1825:1832	arg1	activities					1811:1820	the activities	1807:1820	the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group	1807:1962	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	8	14	from	intensity	1196:1204	arg1	group					1234:1238	each group	1229:1238	each group	1229:1238	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	9	15	theme	precorneal	1404:1413	arg1	time					1425:1428	precorneal residence time	1404:1428	precorneal residence time	1404:1428	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	4	16	theme	transmission	705:716	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy	705:736	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	5	17	theme	BA	925:926	arg1	NPs					932:934	BA LCH NPs	925:934	BA LCH NPs	925:934	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	11	18	theme	positive	1909:1916	arg1	control					1918:1924	positive control	1909:1924	positive control	1909:1924	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	4	19	theme	microscopy	727:736	arg1	data					639:642	The analytical data	624:642	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy	624:736	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	5	20	theme	NPs	932:934	arg1	retention					912:920	the precorneal retention	897:920	the precorneal retention of BA LCH NPs	897:934	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	5	20	theme	NPs	932:934	arg1	irritation					854:863	potential ocular irritation	837:863	potential ocular irritation	837:863	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	5	20	theme	NPs	932:934	arg1	permeability					879:890	transcorneal permeability	866:890	transcorneal permeability	866:890	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	5	20	theme	NPs	932:934	arg1	viability					809:817	cell viability	804:817	cell viability	804:817	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	5	20	theme	NPs	932:934	arg1	uptake					829:834	cellular uptake	820:834	cellular uptake	820:834	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	6	21	theme	therapeutic	1009:1019	arg1	effect					1021:1026	the therapeutic effect	1005:1026	the therapeutic effect of BA LCH NPs	1005:1040	In vivo, the chronic selenium cataract model was selected to assess the therapeutic effect of BA LCH NPs.
36067066	2	22	theme	drug	513:516	arg1	loading					518:524	drug loading	513:524	drug loading	513:524	mPEG-PLGA NPs were optimized by the Box-Behnken design and the central composite design based on the encapsulation efficiency and drug loading.
36067066	4	23	theme	X-ray	682:686	arg1	diffraction					688:698	X-ray diffraction	682:698	X-ray diffraction	682:698	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	11	24	theme	therapeutic	1688:1698	arg1	efficacy					1700:1707	therapeutic efficacy	1688:1707	therapeutic efficacy	1688:1707	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	7	25	from	nm	1103:1104	arg1	mV					1139:1140	19-25 mV	1133:1140	19-25 mV	1133:1140	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	7	25	from	nm	1103:1104	arg1	range					1081:1085	the range	1077:1085	the range from 148 to 219 nm	1077:1104	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	7	25	from	nm	1103:1104	arg1	potential					1119:1127	the zeta potential	1110:1127	the zeta potential	1110:1127	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	4	26	theme	drug	751:754	arg1	compatibility					766:778	the drug excipient compatibility	747:778	the drug excipient compatibility	747:778	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	11	27	theme	glutathione	1861:1871	arg1	peroxidase					1873:1882	glutathione peroxidase	1861:1882	glutathione peroxidase	1861:1882	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	1	28	theme	cataract	373:380	arg1	treatment					360:368	the treatment	356:368	the treatment of cataract	356:380	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	2	29	theme	composite	454:462	arg1	design					464:469	the central composite design	442:469	the central composite design	442:469	mPEG-PLGA NPs were optimized by the Box-Behnken design and the central composite design based on the encapsulation efficiency and drug loading.
36067066	7	30	dep	219	1099:1101	arg1	to					1096:1097	to	1096:1097	to	1096:1097	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	8	31	theme	NP	1303:1304	arg1	group					1306:1310	the LCH NP group	1295:1310	the LCH NP group	1295:1310	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	1	32	dep	poly	257:260	arg1	-poly					278:282	-poly	278:282	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	32	dep	poly	257:260	arg1	d					284:284	d	284:284	d	284:284	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	32	dep	poly	257:260	arg1	acid					307:310	l-lactic-co-glycolic acid	286:310	l-lactic-co-glycolic acid	286:310	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	10	33	theme	fluorescence	1566:1577	arg1	tracking					1579:1586	fluorescence tracking	1566:1586	fluorescence tracking on the rabbit cornea	1566:1607	Also, fluorescence tracking on the rabbit cornea showed increased corneal retention of the LCH NPs.
36067066	9	34	theme	BA	1500:1501	arg1	release					1489:1495	a sustained release	1477:1495	a sustained release of BA through the cornea for effective management of cataract	1477:1557	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	4	35	theme	excipient	756:764	arg1	compatibility					766:778	the drug excipient compatibility	747:778	the drug excipient compatibility	747:778	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	6	36	theme	LCH	1034:1036	arg1	NPs					1038:1040	BA LCH NPs	1031:1040	BA LCH NPs	1031:1040	In vivo, the chronic selenium cataract model was selected to assess the therapeutic effect of BA LCH NPs.
36067066	11	37	theme	dismutase	1846:1854	arg1	activities					1811:1820	the activities	1807:1820	the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group	1807:1962	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	4	38	theme	analytical	628:637	arg1	data					639:642	The analytical data	624:642	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy	624:736	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	10	39	theme	rabbit	1595:1600	arg1	cornea					1602:1607	the rabbit cornea	1591:1607	the rabbit cornea	1591:1607	Also, fluorescence tracking on the rabbit cornea showed increased corneal retention of the LCH NPs.
36067066	0	40	theme	Low-Molecular-Weight	37:56	arg1	mPEG-PLGA					83:91	Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA	37:91	Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA	37:91	Optimization and Characterization of Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA Nanoparticles for the Treatment of Cataract.
36067066	1	41	theme	nanoparticles	325:337	arg1	effectiveness					186:198	the potential effectiveness	172:198	the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract	172:380	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	12	42	theme	BA	2094:2095	arg1	administration					2076:2089	the ophthalmic administration	2061:2089	the ophthalmic administration of BA to the posterior segment of the eye	2061:2131	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	5	43	theme	cell	804:807	arg1	viability					809:817	cell viability	804:817	cell viability	804:817	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	1	44	theme	BA	340:341	arg1	NPs					347:349	BA LCH NPs	340:349	BA LCH NPs	340:349	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	44	theme	BA	340:341	arg1	nanoparticles					325:337	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles	203:337	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	10	45	theme	corneal	1626:1632	arg1	retention					1634:1642	increased corneal retention	1616:1642	increased corneal retention of the LCH NPs	1616:1657	Also, fluorescence tracking on the rabbit cornea showed increased corneal retention of the LCH NPs.
36067066	9	46	theme	cataract	1550:1557	arg1	management					1536:1545	effective management	1526:1545	effective management of cataract	1526:1557	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	5	47	theme	transcorneal	866:877	arg1	permeability					879:890	transcorneal permeability	866:890	transcorneal permeability	866:890	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	10	48	theme	LCH	1651:1653	arg1	NPs					1655:1657	the LCH NPs	1647:1657	the LCH NPs	1647:1657	Also, fluorescence tracking on the rabbit cornea showed increased corneal retention of the LCH NPs.
36067066	3	49	theme	LCH	540:542	arg1	NPs					544:546	the BA LCH NPs	533:546	the BA LCH NPs	533:546	Then, the BA LCH NPs were characterized based on morphology, particle size, and zeta potentials.
36067066	1	50	theme	l-lactic-co-glycolic	286:305	arg1	d					284:284	d	284:284	d	284:284	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	50	theme	l-lactic-co-glycolic	286:305	arg1	acid					307:310	l-lactic-co-glycolic acid	286:310	l-lactic-co-glycolic acid	286:310	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	5	51	theme	precorneal	901:910	arg1	retention					912:920	the precorneal retention	897:920	the precorneal retention of BA LCH NPs	897:934	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	7	52	theme	NPs	1062:1064	arg1	size					1047:1050	The size	1043:1050	The size of BA LCH NPs	1043:1064	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	9	53	theme	optimized	1374:1382	arg1	NPs					1391:1393	The optimized BA LCH NPs	1370:1393	The optimized BA LCH NPs	1370:1393	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	0	54	theme	Baicalin	74:81	arg1	mPEG-PLGA					83:91	Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA	37:91	Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA	37:91	Optimization and Characterization of Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA Nanoparticles for the Treatment of Cataract.
36067066	12	55	theme	delivery	2031:2038	arg1	NPs					2006:2008	the BA LCH NPs	1995:2008	the BA LCH NPs	1995:2008	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	12	55	theme	delivery	2031:2038	arg1	system					2040:2045	a promising drug delivery system	2014:2045	a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms	2014:2163	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	7	56	theme	BA	1055:1056	arg1	NPs					1062:1064	BA LCH NPs	1055:1064	BA LCH NPs	1055:1064	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	1	57	theme	chitosan-coated	224:238	arg1	mPEG-PLGA					314:322	mPEG-PLGA	314:322	mPEG-PLGA	314:322	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	57	theme	chitosan-coated	224:238	arg1	poly					257:260	low-molecular-weight chitosan-coated baicalin methoxy poly	203:260	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	57	theme	chitosan-coated	224:238	arg1	glycol					271:276	ethylene glycol	262:276	ethylene glycol	262:276	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	6	58	theme	cataract	967:974	arg1	model					976:980	the chronic selenium cataract model	946:980	the chronic selenium cataract model	946:980	In vivo, the chronic selenium cataract model was selected to assess the therapeutic effect of BA LCH NPs.
36067066	8	59	theme	fluorescence	1183:1194	arg1	intensity					1196:1204	the fluorescence intensity	1179:1204	the fluorescence intensity of the preparations in each group	1179:1238	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	11	60	theme	LCH	1730:1732	arg1	NPs					1734:1736	BA LCH NPs	1727:1736	BA LCH NPs	1727:1736	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	11	61	theme	BA	1946:1947	arg1	group					1958:1962	the BA solution group	1942:1962	the BA solution group	1942:1962	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	9	62	theme	LCH	1387:1389	arg1	NPs					1391:1393	The optimized BA LCH NPs	1370:1393	The optimized BA LCH NPs	1370:1393	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	6	63	theme	chronic	950:956	arg1	model					976:980	the chronic selenium cataract model	946:980	the chronic selenium cataract model	946:980	In vivo, the chronic selenium cataract model was selected to assess the therapeutic effect of BA LCH NPs.
36067066	12	64	theme	BA	1999:2000	arg1	NPs					2006:2008	the BA LCH NPs	1995:2008	the BA LCH NPs	1995:2008	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	12	64	theme	BA	1999:2000	arg1	system					2040:2045	a promising drug delivery system	2014:2045	a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms	2014:2163	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	3	65	theme	particle	588:595	arg1	size					597:600	particle size	588:600	particle size	588:600	Then, the BA LCH NPs were characterized based on morphology, particle size, and zeta potentials.
36067066	1	66	theme	baicalin	240:247	arg1	mPEG-PLGA					314:322	mPEG-PLGA	314:322	mPEG-PLGA	314:322	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	66	theme	baicalin	240:247	arg1	poly					257:260	low-molecular-weight chitosan-coated baicalin methoxy poly	203:260	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	66	theme	baicalin	240:247	arg1	glycol					271:276	ethylene glycol	262:276	ethylene glycol	262:276	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	5	67	theme	ocular	847:852	arg1	irritation					854:863	potential ocular irritation	837:863	potential ocular irritation	837:863	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	8	68	theme	Cellular	1143:1150	arg1	results					1159:1165	Cellular uptake results	1143:1165	Cellular uptake results	1143:1165	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	8	69	theme	preparations	1213:1224	arg1	intensity					1196:1204	the fluorescence intensity	1179:1204	the fluorescence intensity of the preparations in each group	1179:1238	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	11	70	theme	peroxidase	1873:1882	arg1	activities					1811:1820	the activities	1807:1820	the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group	1807:1962	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	9	71	theme	residence	1415:1423	arg1	time					1425:1428	precorneal residence time	1404:1428	precorneal residence time	1404:1428	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	3	72	theme	zeta	607:610	arg1	potentials					612:621	zeta potentials	607:621	zeta potentials	607:621	Then, the BA LCH NPs were characterized based on morphology, particle size, and zeta potentials.
36067066	5	73	theme	LCH	928:930	arg1	NPs					932:934	BA LCH NPs	925:934	BA LCH NPs	925:934	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	4	74	theme	electron	718:725	arg1	microscopy					727:736	transmission electron microscopy	705:736	transmission electron microscopy	705:736	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	1	75	theme	methoxy	249:255	arg1	mPEG-PLGA					314:322	mPEG-PLGA	314:322	mPEG-PLGA	314:322	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	75	theme	methoxy	249:255	arg1	poly					257:260	low-molecular-weight chitosan-coated baicalin methoxy poly	203:260	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	75	theme	methoxy	249:255	arg1	glycol					271:276	ethylene glycol	262:276	ethylene glycol	262:276	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	9	76	theme	eye	1446:1448	arg1	irritation					1450:1459	eye irritation	1446:1459	eye irritation	1446:1459	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	1	77	theme	potential	176:184	arg1	effectiveness					186:198	the potential effectiveness	172:198	the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract	172:380	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	2	78	theme	encapsulation	484:496	arg1	efficiency					498:507	the encapsulation efficiency	480:507	the encapsulation efficiency	480:507	mPEG-PLGA NPs were optimized by the Box-Behnken design and the central composite design based on the encapsulation efficiency and drug loading.
36067066	4	79	theme	diffraction	688:698	arg1	data					639:642	The analytical data	624:642	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy	624:736	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	11	80	theme	efficacy	1700:1707	arg1	results					1677:1683	the results	1673:1683	the results of therapeutic efficacy	1673:1707	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	0	81	theme	Cataract	128:135	arg1	Treatment					115:123	the Treatment	111:123	the Treatment of Cataract	111:135	Optimization and Characterization of Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA Nanoparticles for the Treatment of Cataract.
36067066	12	82	theme	cataract	2147:2154	arg1	symptoms					2156:2163	cataract symptoms	2147:2163	cataract symptoms	2147:2163	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	8	83	theme	fluorescence	1269:1280	arg1	intensity					1282:1290	the fluorescence intensity	1265:1290	the fluorescence intensity of the LCH NP group	1265:1310	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	8	83	theme	fluorescence	1269:1280	arg1	higher					1330:1335	higher	1330:1335	higher	1330:1335	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	1	84	theme	poly	257:260	arg1	NPs					347:349	BA LCH NPs	340:349	BA LCH NPs	340:349	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	84	theme	poly	257:260	arg1	nanoparticles					325:337	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles	203:337	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	9	85	theme	sustained	1479:1487	arg1	release					1489:1495	a sustained release	1477:1495	a sustained release of BA through the cornea for effective management of cataract	1477:1557	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	12	86	theme	eye	2129:2131	arg1	segment					2114:2120	the posterior segment	2100:2120	the posterior segment of the eye	2100:2131	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	11	87	theme	superoxide	1835:1844	arg1	dismutase					1846:1854	superoxide dismutase	1835:1854	superoxide dismutase	1835:1854	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	7	88	theme	zeta	1114:1117	arg1	mV					1139:1140	19-25 mV	1133:1140	19-25 mV	1133:1140	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	7	88	theme	zeta	1114:1117	arg1	potential					1119:1127	the zeta potential	1110:1127	the zeta potential	1110:1127	The size of BA LCH NPs was within the range from 148 to 219 nm and the zeta potential was 19-25 mV.
36067066	2	89	theme	central	446:452	arg1	design					464:469	the central composite design	442:469	the central composite design	442:469	mPEG-PLGA NPs were optimized by the Box-Behnken design and the central composite design based on the encapsulation efficiency and drug loading.
36067066	8	90	theme	LCH	1299:1301	arg1	group					1306:1310	the LCH NP group	1295:1310	the LCH NP group	1295:1310	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	2	91	theme	mPEG-PLGA	383:391	arg1	NPs					393:395	mPEG-PLGA NPs	383:395	mPEG-PLGA NPs	383:395	mPEG-PLGA NPs were optimized by the Box-Behnken design and the central composite design based on the encapsulation efficiency and drug loading.
36067066	5	92	theme	cellular	820:827	arg1	uptake					829:834	cellular uptake	820:834	cellular uptake	820:834	In vitro, we evaluated cell viability, cellular uptake, potential ocular irritation, transcorneal permeability, and the precorneal retention of BA LCH NPs.
36067066	6	93	theme	NPs	1038:1040	arg1	effect					1021:1026	the therapeutic effect	1005:1026	the therapeutic effect of BA LCH NPs	1005:1040	In vivo, the chronic selenium cataract model was selected to assess the therapeutic effect of BA LCH NPs.
36067066	12	94	theme	drug	2026:2029	arg1	NPs					2006:2008	the BA LCH NPs	1995:2008	the BA LCH NPs	1995:2008	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	12	94	theme	drug	2026:2029	arg1	system					2040:2045	a promising drug delivery system	2014:2045	a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms	2014:2163	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	8	95	theme	group	1306:1310	arg1	intensity					1282:1290	the fluorescence intensity	1265:1290	the fluorescence intensity of the LCH NP group	1265:1310	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	8	95	theme	group	1306:1310	arg1	higher					1330:1335	higher	1330:1335	higher	1330:1335	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	2	96	theme	Box-Behnken	419:429	arg1	design					431:436	the Box-Behnken design	415:436	the Box-Behnken design	415:436	mPEG-PLGA NPs were optimized by the Box-Behnken design and the central composite design based on the encapsulation efficiency and drug loading.
36067066	0	97	theme	Chitosan-Coated	58:72	arg1	mPEG-PLGA					83:91	Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA	37:91	Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA	37:91	Optimization and Characterization of Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA Nanoparticles for the Treatment of Cataract.
36067066	6	98	theme	BA	1031:1032	arg1	NPs					1038:1040	BA LCH NPs	1031:1040	BA LCH NPs	1031:1040	In vivo, the chronic selenium cataract model was selected to assess the therapeutic effect of BA LCH NPs.
36067066	12	99	theme	posterior	2104:2112	arg1	segment					2114:2120	the posterior segment	2100:2120	the posterior segment of the eye	2100:2131	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	12	100	theme	ophthalmic	2065:2074	arg1	administration					2076:2089	the ophthalmic administration	2061:2089	the ophthalmic administration of BA to the posterior segment of the eye	2061:2131	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	0	101	theme	mPEG-PLGA	83:91	arg1	Characterization					17:32	Characterization	17:32	Characterization	17:32	Optimization and Characterization of Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA Nanoparticles for the Treatment of Cataract.
36067066	0	101	theme	mPEG-PLGA	83:91	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization and Characterization of Low-Molecular-Weight Chitosan-Coated Baicalin mPEG-PLGA Nanoparticles for the Treatment of Cataract.
36067066	9	102	theme	effective	1526:1534	arg1	management					1536:1545	effective management	1526:1545	effective management of cataract	1526:1557	The optimized BA LCH NPs improved precorneal residence time without causing eye irritation and also showed a sustained release of BA through the cornea for effective management of cataract.
36067066	12	103	theme	promising	2016:2024	arg1	NPs					2006:2008	the BA LCH NPs	1995:2008	the BA LCH NPs	1995:2008	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	12	103	theme	promising	2016:2024	arg1	system					2040:2045	a promising drug delivery system	2014:2045	a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms	2014:2163	Thus, it can be inferred that the BA LCH NPs are a promising drug delivery system for enhancing the ophthalmic administration of BA to the posterior segment of the eye and improving cataract symptoms.
36067066	11	104	theme	malondialdehyde	1778:1792	arg1	content					1767:1773	the content	1763:1773	the content of malondialdehyde	1763:1792	In addition, the results of therapeutic efficacy demonstrated that BA LCH NPs can significantly reduce the content of malondialdehyde and enhanced the activities of catalase, superoxide dismutase, and glutathione peroxidase, which was comparable to positive control and better than the BA solution group.
36067066	1	105	theme	LCH	343:345	arg1	NPs					347:349	BA LCH NPs	340:349	BA LCH NPs	340:349	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	105	theme	LCH	343:345	arg1	nanoparticles					325:337	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles	203:337	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	4	106	theme	scanning	660:667	arg1	calorimetry					669:679	differential scanning calorimetry	647:679	differential scanning calorimetry	647:679	The analytical data of differential scanning calorimetry, X-ray diffraction, and transmission electron microscopy depicted the drug excipient compatibility.
36067066	10	107	from	tracking	1579:1586	arg1	cornea					1602:1607	the rabbit cornea	1591:1607	the rabbit cornea	1591:1607	Also, fluorescence tracking on the rabbit cornea showed increased corneal retention of the LCH NPs.
36067066	10	108	theme	increased	1616:1624	arg1	retention					1634:1642	increased corneal retention	1616:1642	increased corneal retention of the LCH NPs	1616:1657	Also, fluorescence tracking on the rabbit cornea showed increased corneal retention of the LCH NPs.
36067066	1	109	theme	ethylene	262:269	arg1	poly					257:260	low-molecular-weight chitosan-coated baicalin methoxy poly	203:260	low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs)	203:350	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	1	109	theme	ethylene	262:269	arg1	glycol					271:276	ethylene glycol	262:276	ethylene glycol	262:276	The present study was to evaluate the potential effectiveness of low-molecular-weight chitosan-coated baicalin methoxy poly(ethylene glycol)-poly(d,l-lactic-co-glycolic acid) (mPEG-PLGA) nanoparticles (BA LCH NPs) for the treatment of cataract.
36067066	8	110	theme	solution	1354:1361	arg1	group					1363:1367	the solution group	1350:1367	the solution group	1350:1367	Cellular uptake results showed that the fluorescence intensity of the preparations in each group increased with time, and the fluorescence intensity of the LCH NP group was significantly higher than that of the solution group.
36067066	10	111	theme	NPs	1655:1657	arg1	retention					1634:1642	increased corneal retention	1616:1642	increased corneal retention of the LCH NPs	1616:1657	Also, fluorescence tracking on the rabbit cornea showed increased corneal retention of the LCH NPs.
37170263	12	0	theme	performing	1490:1499	arg1	extract					1507:1513	poorly performing yeast extract	1483:1513	poorly performing yeast extract	1483:1513	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	4	1	theme	biological	565:574	arg1	cultivations					576:587	biological cultivations	565:587	biological cultivations	565:587	These variations can have an immense impact on the results of biological cultivations.
37170263	2	2	theme	complex	345:351	arg1	substrates					353:362	complex substrates	345:362	complex substrates	345:362	For microbial fermentations, complex substrates and media components are often used.
37170263	19	3	theme	media	2383:2387	arg1	potential					2473:2481	the potential	2469:2481	the potential of targeted supplementation of the media	2469:2522	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	19	3	theme	media	2383:2387	arg1	potential					2359:2367	the potential	2355:2367	the potential of optimising media containing yeast extract for alginate production with A. vinelandii	2355:2455	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	10	4	theme	performing	1247:1256	arg1	extracts					1264:1271	poorly performing yeast extracts	1240:1271	poorly performing yeast extracts	1240:1271	Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration.
37170263	15	5	theme	yeast	1878:1882	arg1	extract					1884:1890	yeast extract	1878:1890	yeast extract	1878:1890	CONCLUSION The choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations.
37170263	13	6	theme	copper	1659:1664	arg1	sulphate					1666:1673	copper sulphate	1659:1673	copper sulphate	1659:1673	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	1	7	theme	high	255:258	arg1	standards					279:287	high quality and safety standards	255:287	high quality and safety standards	255:287	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	4	8	contain	have	524:527	arg2	impact					540:545	an immense impact	529:545	an immense impact	529:545	These variations can have an immense impact on the results of biological cultivations.
37170263	4	8	contain	have	524:527	arg1	variations					509:518	These variations	503:518	These variations	503:518	These variations can have an immense impact on the results of biological cultivations.
37170263	11	9	theme	yeast	1383:1387	arg1	extracts					1389:1396	well-performing yeast extracts	1367:1396	well-performing yeast extracts	1367:1396	On the other hand, the results with well-performing yeast extracts seem to reach a saturation, when their concentration is increased.
37170263	5	10	theme	yeast	692:696	arg1	extract					698:704	the complex media component yeast extract	664:704	the complex media component yeast extract	664:704	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	19	11	contain	containing	2389:2398	arg2	extract					2406:2412	yeast extract	2400:2412	yeast extract	2400:2412	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	19	11	contain	containing	2389:2398	arg1	media					2383:2387	optimising media	2372:2387	optimising media containing yeast extract for alginate production with A. vinelandii	2372:2455	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	0	12	theme	alginate	128:135	arg1	production					137:146	alginate production	128:146	alginate production by A. vinelandii ATCC9046	128:172	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	14	13	theme	accumulated	1750:1760	arg1	transfer					1769:1776	the accumulated oxygen transfer	1746:1776	the accumulated oxygen transfer	1746:1776	Furthermore, a correlation between the accumulated oxygen transfer and the final viscosity (as a key performance indicator), was established.
37170263	13	14	theme	Beneficial	1598:1607	arg1	results					1609:1615	Beneficial results	1598:1615	Beneficial results	1598:1615	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	18	15	theme	mechanisms	2255:2264	arg1	surface					2229:2235	the surface	2225:2235	the surface of the underlying mechanisms	2225:2264	The results only scratch the surface of the underlying mechanisms, as they are not providing explanations on a molecular level.
37170263	15	16	theme	vinelandii	1910:1919	arg1	cultivations					1921:1932	A. vinelandii cultivations	1907:1932	A. vinelandii cultivations	1907:1932	CONCLUSION The choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations.
37170263	1	17	from	factor	239:244	arg1	production					202:211	production	202:211	production	202:211	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	1	17	from	factor	239:244	arg1	research					189:196	research	189:196	research	189:196	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	8	18	dep	extracts	965:972	arg1	manufacturers					991:1003	6 different manufacturers	979:1003	6 different manufacturers	979:1003	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	8	18	dep	extracts	965:972	arg1	lots					1021:1024	2 different lots	1009:1024	2 different lots	1009:1024	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	5	19	theme	media	676:680	arg1	extract					698:704	the complex media component yeast extract	664:704	the complex media component yeast extract	664:704	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	10	20	theme	extracts	1264:1271	arg1	performance					1225:1235	the performance	1221:1235	the performance of poorly performing yeast extracts	1221:1271	Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration.
37170263	5	21	theme	microaerobic	754:765	arg1	conditions					767:776	microaerobic conditions	754:776	microaerobic conditions	754:776	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	10	22	theme	Concentration	1150:1162	arg1	variations					1164:1173	Concentration variations	1150:1173	Concentration variations of three different yeast extracts	1150:1207	Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration.
37170263	2	23	used	used	395:398	arg2	media					368:372	media	368:372	media	368:372	For microbial fermentations, complex substrates and media components are often used.
37170263	2	23	used	used	395:398	arg2	substrates					353:362	complex substrates	345:362	complex substrates	345:362	For microbial fermentations, complex substrates and media components are often used.
37170263	9	24	theme	Significant	1064:1074	arg1	differences					1076:1086	Significant differences	1064:1086	Significant differences on respiratory activity, growth and production	1064:1133	Significant differences on respiratory activity, growth and production were observed.
37170263	2	25	dep	substrates	353:362	arg1	components					374:383	components	374:383	components	374:383	For microbial fermentations, complex substrates and media components are often used.
37170263	0	26	theme	elements	89:96	arg1	supplementation					48:62	supplementation	48:62	supplementation of amino acids and micro elements	48:96	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	0	26	theme	elements	89:96	arg1	manufacturers					30:42	different manufacturers	20:42	different manufacturers	20:42	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	16	27	theme	specific	2021:2028	arg1	extracts					2036:2043	specific yeast extracts	2021:2043	specific yeast extracts	2021:2043	The proper use of specific yeast extracts allows the cultivation results to be specifically optimised.
37170263	12	28	theme	micro	1582:1586	arg1	elements					1588:1595	micro elements	1582:1595	micro elements	1582:1595	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	14	29	theme	final	1786:1790	arg1	viscosity					1792:1800	the final viscosity	1782:1800	the final viscosity (as a key performance indicator)	1782:1833	Furthermore, a correlation between the accumulated oxygen transfer and the final viscosity (as a key performance indicator), was established.
37170263	0	30	from	supplementation	48:62	arg1	extracts					6:13	Yeast extracts	0:13	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements	0:96	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	18	31	from	explanations	2293:2304	arg1	level					2321:2325	a molecular level	2309:2325	a molecular level	2309:2325	The results only scratch the surface of the underlying mechanisms, as they are not providing explanations on a molecular level.
37170263	16	32	theme	extracts	2036:2043	arg1	use					2014:2016	The proper use	2003:2016	The proper use of specific yeast extracts	2003:2043	The proper use of specific yeast extracts allows the cultivation results to be specifically optimised.
37170263	0	33	from	manufacturers	30:42	arg1	extracts					6:13	Yeast extracts	0:13	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements	0:96	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	11	34	theme	other	1338:1342	arg1	hand					1344:1347	the other hand	1334:1347	the other hand	1334:1347	On the other hand, the results with well-performing yeast extracts seem to reach a saturation, when their concentration is increased.
37170263	11	35	with	results	1354:1360	arg1	extracts					1389:1396	well-performing yeast extracts	1367:1396	well-performing yeast extracts	1367:1396	On the other hand, the results with well-performing yeast extracts seem to reach a saturation, when their concentration is increased.
37170263	0	36	theme	Yeast	0:4	arg1	extracts					6:13	Yeast extracts	0:13	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements	0:96	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	1	37	theme	quality	260:266	arg1	standards					279:287	high quality and safety standards	255:287	high quality and safety standards	255:287	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	18	38	theme	molecular	2311:2319	arg1	level					2321:2325	a molecular level	2309:2325	a molecular level	2309:2325	The results only scratch the surface of the underlying mechanisms, as they are not providing explanations on a molecular level.
37170263	1	39	theme	safety	272:277	arg1	standards					279:287	high quality and safety standards	255:287	high quality and safety standards	255:287	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	8	40	from	extracts	965:972	arg1	RESULTS					951:957	RESULTS	951:957	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer	951:1046	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	3	41	theme	complex	405:411	arg1	components					419:428	The complex media components	401:428	The complex media components	401:428	The complex media components can vary in composition, depending on the lot and manufacturing process.
37170263	10	42	theme	extracts	1200:1207	arg1	variations					1164:1173	Concentration variations	1150:1173	Concentration variations of three different yeast extracts	1150:1207	Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration.
37170263	12	43	theme	amino	1566:1570	arg1	acids					1572:1576	single amino acids	1559:1576	single amino acids	1559:1576	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	12	44	with	Cultivations	1465:1476	arg1	extract					1507:1513	poorly performing yeast extract	1483:1513	poorly performing yeast extract	1483:1513	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	19	45	theme	supplementation	2495:2509	arg1	potential					2473:2481	the potential	2469:2481	the potential of targeted supplementation of the media	2469:2522	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	19	45	theme	supplementation	2495:2509	arg1	potential					2359:2367	the potential	2355:2367	the potential of optimising media containing yeast extract for alginate production with A. vinelandii	2355:2455	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	13	46	theme	cysteine	1676:1683	arg1	supplementation					1640:1654	the supplementation	1636:1654	the supplementation of copper sulphate, cysteine or a combination of both	1636:1708	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	0	47	theme	amino	67:71	arg1	acids					73:77	amino acids	67:77	amino acids	67:77	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	10	48	theme	different	1184:1192	arg1	extracts					1200:1207	three different yeast extracts	1178:1207	three different yeast extracts	1178:1207	Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration.
37170263	2	49	theme	microbial	320:328	arg1	fermentations					330:342	microbial fermentations	320:342	microbial fermentations	320:342	For microbial fermentations, complex substrates and media components are often used.
37170263	19	50	theme	optimising	2372:2381	arg1	media					2383:2387	optimising media	2372:2387	optimising media containing yeast extract for alginate production with A. vinelandii	2372:2455	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	14	51	theme	key	1808:1810	arg1	indicator					1824:1832	a key performance indicator	1806:1832	a key performance indicator	1806:1832	Furthermore, a correlation between the accumulated oxygen transfer and the final viscosity (as a key performance indicator), was established.
37170263	12	52	theme	amino	1546:1550	arg1	acids					1552:1556	grouped amino acids	1538:1556	grouped amino acids	1538:1556	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	8	53	theme	Yeast	959:963	arg1	extracts					965:972	Yeast extracts	959:972	Yeast extracts	959:972	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	1	54	theme	key	235:237	arg1	factor					239:244	a key factor	233:244	a key factor	233:244	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	1	54	theme	key	235:237	arg1	reproducibility					214:228	reproducibility	214:228	reproducibility	214:228	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	12	55	theme	yeast	1501:1505	arg1	extract					1507:1513	poorly performing yeast extract	1483:1513	poorly performing yeast extract	1483:1513	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	5	56	from	influence	651:659	arg1	cultivations					709:720	cultivations	709:720	cultivations of Azotobacter vinelandii	709:746	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	15	57	theme	extract	1884:1890	arg1	crucial					1895:1901	crucial	1895:1901	crucial	1895:1901	CONCLUSION The choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations.
37170263	15	57	theme	extract	1884:1890	arg1	choice					1868:1873	The choice	1864:1873	The choice of yeast extract	1864:1890	CONCLUSION The choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations.
37170263	7	58	theme	respiration	892:902	arg1	activity					904:911	the respiration activity	888:911	the respiration activity	888:911	The focus of the investigation was on the respiration activity, cell growth and alginate production.
37170263	10	59	theme	yeast	1258:1262	arg1	extracts					1264:1271	poorly performing yeast extracts	1240:1271	poorly performing yeast extracts	1240:1271	Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration.
37170263	8	60	theme	different	981:989	arg1	manufacturers					991:1003	6 different manufacturers	979:1003	6 different manufacturers	979:1003	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	18	61	theme	providing	2283:2291	arg1	explanations					2293:2304	providing explanations	2283:2304	providing explanations on a molecular level	2283:2325	The results only scratch the surface of the underlying mechanisms, as they are not providing explanations on a molecular level.
37170263	18	61	theme	providing	2283:2291	arg1	they					2270:2273	they	2270:2273	they	2270:2273	The results only scratch the surface of the underlying mechanisms, as they are not providing explanations on a molecular level.
37170263	4	62	theme	cultivations	576:587	arg1	results					554:560	the results	550:560	the results of biological cultivations	550:587	These variations can have an immense impact on the results of biological cultivations.
37170263	5	63	theme	work	606:609	arg1	aim					594:596	The aim	590:596	The aim of this work	590:609	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	13	64	theme	sulphate	1666:1673	arg1	supplementation					1640:1654	the supplementation	1636:1654	the supplementation of copper sulphate, cysteine or a combination of both	1636:1708	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	7	65	theme	investigation	867:879	arg1	focus					854:858	The focus	850:858	The focus of the investigation	850:879	The focus of the investigation was on the respiration activity, cell growth and alginate production.
37170263	8	66	theme	different	1011:1019	arg1	lots					1021:1024	2 different lots	1009:1024	2 different lots	1009:1024	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	11	67	theme	well-performing	1367:1381	arg1	extracts					1389:1396	well-performing yeast extracts	1367:1396	well-performing yeast extracts	1367:1396	On the other hand, the results with well-performing yeast extracts seem to reach a saturation, when their concentration is increased.
37170263	19	68	theme	alginate	2418:2425	arg1	production					2427:2436	alginate production	2418:2436	alginate production with A. vinelandii	2418:2455	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	5	69	theme	component	682:690	arg1	extract					698:704	the complex media component yeast extract	664:704	the complex media component yeast extract	664:704	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	17	70	theme	alginate	2190:2197	arg1	properties					2172:2181	the properties	2168:2181	the properties of the alginate	2168:2197	In addition, supplements can be applied to modify and improve the properties of the alginate.
37170263	8	71	from	manufacturer	1035:1046	arg1	manufacturers					991:1003	6 different manufacturers	979:1003	6 different manufacturers	979:1003	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	8	71	from	manufacturer	1035:1046	arg1	lots					1021:1024	2 different lots	1009:1024	2 different lots	1009:1024	RESULTS Yeast extracts from 6 different manufacturers and 2 different lots from one manufacturer were evaluated.
37170263	5	72	theme	extract	698:704	arg1	influence					651:659	the influence	647:659	the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii	647:746	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	14	73	theme	oxygen	1762:1767	arg1	transfer					1769:1776	the accumulated oxygen transfer	1746:1776	the accumulated oxygen transfer	1746:1776	Furthermore, a correlation between the accumulated oxygen transfer and the final viscosity (as a key performance indicator), was established.
37170263	19	74	theme	targeted	2486:2493	arg1	supplementation					2495:2509	targeted supplementation	2486:2509	targeted supplementation of the media	2486:2522	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	15	75	theme	A.	1907:1908	arg1	cultivations					1921:1932	A. vinelandii cultivations	1907:1932	A. vinelandii cultivations	1907:1932	CONCLUSION The choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations.
37170263	18	76	theme	underlying	2244:2253	arg1	mechanisms					2255:2264	the underlying mechanisms	2240:2264	the underlying mechanisms	2240:2264	The results only scratch the surface of the underlying mechanisms, as they are not providing explanations on a molecular level.
37170263	5	77	theme	complex	668:674	arg1	media					676:680	complex media	668:680	the complex media component yeast extract	664:704	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	15	78	dep	CONCLUSION	1853:1862	arg1	crucial					1895:1901	crucial	1895:1901	crucial	1895:1901	CONCLUSION The choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations.
37170263	15	78	dep	CONCLUSION	1853:1862	arg1	choice					1868:1873	The choice	1864:1873	The choice of yeast extract	1864:1890	CONCLUSION The choice of yeast extract is crucial for A. vinelandii cultivations, to maintain reproducibility and comparability between cultivations.
37170263	9	79	from	differences	1076:1086	arg1	production					1124:1133	production	1124:1133	production	1124:1133	Significant differences on respiratory activity, growth and production were observed.
37170263	9	79	from	differences	1076:1086	arg1	growth					1113:1118	growth	1113:1118	growth	1113:1118	Significant differences on respiratory activity, growth and production were observed.
37170263	9	79	from	differences	1076:1086	arg1	activity					1103:1110	respiratory activity	1091:1110	respiratory activity	1091:1110	Significant differences on respiratory activity, growth and production were observed.
37170263	0	80	theme	acids	73:77	arg1	supplementation					48:62	supplementation	48:62	supplementation of amino acids and micro elements	48:96	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	0	80	theme	acids	73:77	arg1	manufacturers					30:42	different manufacturers	20:42	different manufacturers	20:42	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	0	81	theme	micro	83:87	arg1	elements					89:96	micro elements	83:96	micro elements	83:96	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	16	82	theme	yeast	2030:2034	arg1	extracts					2036:2043	specific yeast extracts	2021:2043	specific yeast extracts	2021:2043	The proper use of specific yeast extracts allows the cultivation results to be specifically optimised.
37170263	0	83	theme	vinelandii	154:163	arg1	ATCC9046					165:172	A. vinelandii ATCC9046	151:172	A. vinelandii ATCC9046	151:172	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	9	84	theme	respiratory	1091:1101	arg1	activity					1103:1110	respiratory activity	1091:1110	respiratory activity	1091:1110	Significant differences on respiratory activity, growth and production were observed.
37170263	0	85	theme	remarkable	107:116	arg1	impact					118:123	a remarkable impact	105:123	a remarkable impact on alginate production by A. vinelandii ATCC9046	105:172	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	5	86	theme	Azotobacter	725:735	arg1	cultivations					709:720	cultivations	709:720	cultivations of Azotobacter vinelandii	709:746	The aim of this work was to investigate and characterize the influence of the complex media component yeast extract on cultivations of Azotobacter vinelandii under microaerobic conditions.
37170263	16	87	theme	cultivation	2056:2066	arg1	results					2068:2074	the cultivation results	2052:2074	the cultivation results	2052:2074	The proper use of specific yeast extracts allows the cultivation results to be specifically optimised.
37170263	6	88	theme	biopolymer	829:838	arg1	alginate					840:847	the biopolymer alginate	825:847	the biopolymer alginate	825:847	Under these conditions, the organism produces the biopolymer alginate.
37170263	0	89	theme	different	20:28	arg1	manufacturers					30:42	different manufacturers	20:42	different manufacturers	20:42	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	7	90	theme	alginate	930:937	arg1	production					939:948	alginate production	930:948	alginate production	930:948	The focus of the investigation was on the respiration activity, cell growth and alginate production.
37170263	13	91	theme	both	1705:1708	arg1	cysteine					1676:1683	cysteine	1676:1683	cysteine	1676:1683	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	13	91	theme	both	1705:1708	arg1	combination					1690:1700	a combination	1688:1700	a combination of both	1688:1708	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	13	91	theme	both	1705:1708	arg1	sulphate					1666:1673	copper sulphate	1659:1673	copper sulphate	1659:1673	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	3	92	theme	media	413:417	arg1	components					419:428	The complex media components	401:428	The complex media components	401:428	The complex media components can vary in composition, depending on the lot and manufacturing process.
37170263	10	93	theme	yeast	1194:1198	arg1	extracts					1200:1207	three different yeast extracts	1178:1207	three different yeast extracts	1178:1207	Concentration variations of three different yeast extracts showed that the performance of poorly performing yeast extracts can be improved by simply increasing their concentration.
37170263	12	94	theme	single	1559:1564	arg1	acids					1572:1576	single amino acids	1559:1576	single amino acids	1559:1576	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	4	95	theme	immense	532:538	arg1	impact					540:545	an immense impact	529:545	an immense impact	529:545	These variations can have an immense impact on the results of biological cultivations.
37170263	1	96	dep	BACKGROUND	175:184	arg1	factor					239:244	a key factor	233:244	a key factor	233:244	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	1	96	dep	BACKGROUND	175:184	arg1	reproducibility					214:228	reproducibility	214:228	reproducibility	214:228	BACKGROUND In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity.
37170263	3	97	theme	manufacturing	480:492	arg1	process					494:500	the lot and manufacturing process	468:500	the lot and manufacturing process	468:500	The complex media components can vary in composition, depending on the lot and manufacturing process.
37170263	7	98	theme	cell	914:917	arg1	growth					919:924	cell growth	914:924	cell growth	914:924	The focus of the investigation was on the respiration activity, cell growth and alginate production.
37170263	14	99	theme	performance	1812:1822	arg1	indicator					1824:1832	a key performance indicator	1806:1832	a key performance indicator	1806:1832	Furthermore, a correlation between the accumulated oxygen transfer and the final viscosity (as a key performance indicator), was established.
37170263	16	100	theme	proper	2007:2012	arg1	use					2014:2016	The proper use	2003:2016	The proper use of specific yeast extracts	2003:2043	The proper use of specific yeast extracts allows the cultivation results to be specifically optimised.
37170263	13	101	theme	combination	1690:1700	arg1	supplementation					1640:1654	the supplementation	1636:1654	the supplementation of copper sulphate, cysteine or a combination of both	1636:1708	Beneficial results were obtained with the supplementation of copper sulphate, cysteine or a combination of both.
37170263	19	102	with	production	2427:2436	arg1	vinelandii					2446:2455	A. vinelandii	2443:2455	A. vinelandii	2443:2455	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	3	103	theme	lot	472:474	arg1	process					494:500	the lot and manufacturing process	468:500	the lot and manufacturing process	468:500	The complex media components can vary in composition, depending on the lot and manufacturing process.
37170263	12	104	theme	grouped	1538:1544	arg1	acids					1552:1556	grouped amino acids	1538:1556	grouped amino acids	1538:1556	Cultivations with poorly performing yeast extract were supplemented with grouped amino acids, single amino acids and micro elements.
37170263	0	105	from	impact	118:123	arg1	production					137:146	alginate production	128:146	alginate production by A. vinelandii ATCC9046	128:172	Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046.
37170263	19	106	theme	yeast	2400:2404	arg1	extract					2406:2412	yeast extract	2400:2412	yeast extract	2400:2412	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37170263	19	107	theme	media	2518:2522	arg1	supplementation					2495:2509	targeted supplementation	2486:2509	targeted supplementation of the media	2486:2522	However, the findings show the potential of optimising media containing yeast extract for alginate production with A. vinelandii, as well as the potential of targeted supplementation of the media.
37024794	5	0	theme	stem	1082:1085	arg1	cells					1087:1091	mesenchymal stem cells	1070:1091	mesenchymal stem cells	1070:1091	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	6	1	theme	glial	1250:1254	arg1	cells					1256:1260	glial cells	1250:1260	glial cells	1250:1260	CONCLUSIONS DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells.
37024794	4	2	theme	homologous	823:832	arg1	growth-factors					850:863	homologous heparin-binding growth-factors	823:863	homologous heparin-binding growth-factors	823:863	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	4	2	theme	homologous	823:832	arg1	Genes					768:772	Genes	768:772	Genes coding for pleiotrophin (PTN) and midkine (MDK)	768:820	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	0	3	theme	growth	76:81	arg1	factors					83:89	heparin-binding growth factors Pleiotrophin and Midkine	60:114	heparin-binding growth factors Pleiotrophin and Midkine	60:114	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	0	3	theme	growth	76:81	arg1	Midkine					108:114	Midkine	108:114	Midkine	108:114	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	0	3	theme	growth	76:81	arg1	Pleiotrophin					91:102	Pleiotrophin	91:102	Pleiotrophin	91:102	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	0	4	from	crosstalk	14:22	arg1	pulp					40:43	adult dental pulp	27:43	adult dental pulp	27:43	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	5	5	theme	DP	1026:1027	arg1	cells					1038:1042	several other DP resident cells	1012:1042	several other DP resident cells	1012:1042	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	1	6	theme	molecular	167:175	arg1	composition					177:187	the cellular and molecular composition	150:187	the cellular and molecular composition of dental pulp (DP)	150:207	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	4	7	from	levels	912:917	arg1	fibroblasts					925:935	DP fibroblasts	922:935	DP fibroblasts	922:935	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	4	8	theme	expression	901:910	arg1	levels					912:917	the highest differential expression levels	876:917	the highest differential expression levels in DP fibroblasts	876:935	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	7	9	theme	regulatory	1339:1348	arg1	functions					1350:1358	major regulatory functions	1333:1358	major regulatory functions	1333:1358	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	10	theme	therapeutic	1401:1411	arg1	role					1386:1389	a potential role	1374:1389	a potential role	1374:1389	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	10	theme	therapeutic	1401:1411	arg1	targets					1413:1419	dental therapeutic targets	1394:1419	dental therapeutic targets	1394:1419	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	11	contain	possess	1325:1331	arg2	functions					1350:1358	major regulatory functions	1333:1358	major regulatory functions	1333:1358	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	11	contain	possess	1325:1331	arg1	factors					1317:1323	fibroblast-derived growth factors	1291:1323	fibroblast-derived growth factors	1291:1323	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	4	12	theme	differential	888:899	arg1	levels					912:917	the highest differential expression levels	876:917	the highest differential expression levels in DP fibroblasts	876:935	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	2	13	dep	tissues	459:465	arg1	to					438:439	to	438:439	to	438:439	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	1	14	theme	tissue	255:260	arg1	homeostasis					262:272	tissue homeostasis	255:272	tissue homeostasis	255:272	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	2	15	theme	bone	504:507	arg1	marrow					509:514	bone marrow	504:514	bone marrow	504:514	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	15	theme	bone	504:507	arg1	transcriptomes					397:410	publicly available single-cell transcriptomes	366:410	publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	366:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	3	16	theme	DP	689:690	arg1	type					722:725	the main cell type	708:725	the main cell type contributing to this expression profile	708:765	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	16	theme	DP	689:690	arg1	fibroblasts					692:702	DP fibroblasts	689:702	DP fibroblasts	689:702	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	17	contain	have	602:605	arg2	profile					632:638	a unique gene expression profile	607:638	a unique gene expression profile	607:638	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	17	contain	have	602:605	arg1	cells					596:600	DP resident cells	584:600	DP resident cells	584:600	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	7	18	contain	have	1369:1372	arg2	targets					1413:1419	dental therapeutic targets	1394:1419	dental therapeutic targets	1394:1419	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	18	contain	have	1369:1372	arg2	role					1386:1389	a potential role	1374:1389	a potential role	1374:1389	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	18	contain	have	1369:1372	arg1	factors					1317:1323	fibroblast-derived growth factors	1291:1323	fibroblast-derived growth factors	1291:1323	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	19	theme	growth	1310:1315	arg1	factors					1317:1323	fibroblast-derived growth factors	1291:1323	fibroblast-derived growth factors	1291:1323	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	1	20	theme	composition	177:187	arg1	knowledge					137:145	BACKGROUND In-depth knowledge	117:145	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP)	117:207	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	1	20	theme	composition	177:187	arg1	crosstalk					217:225	the crosstalk	213:225	the crosstalk between DP cells that drive tissue homeostasis	213:272	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	6	21	theme	CONCLUSIONS	1136:1146	arg1	fibroblasts					1151:1161	CONCLUSIONS DP fibroblasts	1136:1161	CONCLUSIONS DP fibroblasts	1136:1161	CONCLUSIONS DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells.
37024794	7	22	theme	dental	1394:1399	arg1	role					1386:1389	a potential role	1374:1389	a potential role	1374:1389	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	22	theme	dental	1394:1399	arg1	targets					1413:1419	dental therapeutic targets	1394:1419	dental therapeutic targets	1394:1419	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	2	23	theme	available	375:383	arg1	marrow					509:514	bone marrow	504:514	bone marrow	504:514	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	23	theme	available	375:383	arg1	skin					543:546	skin	543:546	skin	543:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	23	theme	available	375:383	arg1	blood					479:483	peripheral blood mononuclear cells	468:501	peripheral blood mononuclear cells	468:501	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	23	theme	available	375:383	arg1	transcriptomes					397:410	publicly available single-cell transcriptomes	366:410	publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	366:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	23	theme	available	375:383	arg1	tissue					525:530	adipose tissue	517:530	adipose tissue	517:530	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	23	theme	available	375:383	arg1	lung					533:536	lung	533:536	lung	533:536	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	24	theme	tissues	459:465	arg1	marrow					509:514	bone marrow	504:514	bone marrow	504:514	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	24	theme	tissues	459:465	arg1	skin					543:546	skin	543:546	skin	543:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	24	theme	tissues	459:465	arg1	blood					479:483	peripheral blood mononuclear cells	468:501	peripheral blood mononuclear cells	468:501	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	24	theme	tissues	459:465	arg1	transcriptomes					397:410	publicly available single-cell transcriptomes	366:410	publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	366:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	24	theme	tissues	459:465	arg1	tissue					525:530	adipose tissue	517:530	adipose tissue	517:530	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	24	theme	tissues	459:465	arg1	lung					533:536	lung	533:536	lung	533:536	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	25	theme	peripheral	468:477	arg1	blood					479:483	peripheral blood mononuclear cells	468:501	peripheral blood mononuclear cells	468:501	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	25	theme	peripheral	468:477	arg1	transcriptomes					397:410	publicly available single-cell transcriptomes	366:410	publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	366:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	0	26	dep	factors	83:89	arg1	factors					83:89	heparin-binding growth factors Pleiotrophin and Midkine	60:114	heparin-binding growth factors Pleiotrophin and Midkine	60:114	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	0	26	dep	factors	83:89	arg1	Midkine					108:114	Midkine	108:114	Midkine	108:114	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	0	26	dep	factors	83:89	arg1	Pleiotrophin					91:102	Pleiotrophin	91:102	Pleiotrophin	91:102	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	5	27	theme	Schwann	1055:1061	arg1	cells					1063:1067	Schwann cells	1055:1067	Schwann cells	1055:1067	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	1	28	theme	dental	192:197	arg1	DP					205:206	DP	205:206	DP	205:206	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	1	28	theme	dental	192:197	arg1	pulp					199:202	dental pulp	192:202	dental pulp (DP)	192:207	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	0	29	theme	Intercellular	0:12	arg1	crosstalk					14:22	Intercellular crosstalk	0:22	Intercellular crosstalk in adult dental pulp	0:43	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	6	30	theme	DP	1198:1199	arg1	homeostasis					1201:1211	DP homeostasis	1198:1211	DP homeostasis	1198:1211	CONCLUSIONS DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells.
37024794	1	31	theme	pulp	199:202	arg1	composition					177:187	the cellular and molecular composition	150:187	the cellular and molecular composition of dental pulp (DP)	150:207	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	2	32	theme	DP	435:436	arg1	tissues					459:465	healthy adult human DP to 5 other reference tissues	415:465	healthy adult human DP to 5 other reference tissues	415:465	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	3	33	theme	main	712:715	arg1	type					722:725	the main cell type	708:725	the main cell type contributing to this expression profile	708:765	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	33	theme	main	712:715	arg1	fibroblasts					692:702	DP fibroblasts	689:702	DP fibroblasts	689:702	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	34	dep	RESULTS	549:555	arg1	revealed					570:577	revealed	570:577	revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile	570:765	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	2	35	theme	adult	423:427	arg1	tissues					459:465	healthy adult human DP to 5 other reference tissues	415:465	healthy adult human DP to 5 other reference tissues	415:465	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	36	theme	human	429:433	arg1	tissues					459:465	healthy adult human DP to 5 other reference tissues	415:465	healthy adult human DP to 5 other reference tissues	415:465	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	3	37	theme	unique	609:614	arg1	profile					632:638	a unique gene expression profile	607:638	a unique gene expression profile	607:638	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	7	38	theme	potential	1376:1384	arg1	role					1386:1389	a potential role	1374:1389	a potential role	1374:1389	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	7	38	theme	potential	1376:1384	arg1	targets					1413:1419	dental therapeutic targets	1394:1419	dental therapeutic targets	1394:1419	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	2	39	theme	healthy	415:421	arg1	tissues					459:465	healthy adult human DP to 5 other reference tissues	415:465	healthy adult human DP to 5 other reference tissues	415:465	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	4	40	theme	highest	880:886	arg1	levels					912:917	the highest differential expression levels	876:917	the highest differential expression levels in DP fibroblasts	876:935	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	3	41	theme	gene	616:619	arg1	profile					632:638	a unique gene expression profile	607:638	a unique gene expression profile	607:638	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	5	42	theme	extensive	965:973	arg1	crosstalk					975:983	extensive crosstalk	965:983	extensive crosstalk	965:983	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	4	43	contain	possessed	866:874	arg1	growth-factors					850:863	homologous heparin-binding growth-factors	823:863	homologous heparin-binding growth-factors	823:863	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	4	43	contain	possessed	866:874	arg1	Genes					768:772	Genes	768:772	Genes coding for pleiotrophin (PTN) and midkine (MDK)	768:820	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	4	43	contain	possessed	866:874	arg2	levels					912:917	the highest differential expression levels	876:917	the highest differential expression levels in DP fibroblasts	876:935	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	0	44	theme	dental	33:38	arg1	pulp					40:43	adult dental pulp	27:43	adult dental pulp	27:43	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	6	45	with	crosstalk	1235:1243	arg1	cells					1256:1260	glial cells	1250:1260	glial cells	1250:1260	CONCLUSIONS DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells.
37024794	2	46	theme	adipose	517:523	arg1	tissue					525:530	adipose tissue	517:530	adipose tissue	517:530	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	46	theme	adipose	517:523	arg1	transcriptomes					397:410	publicly available single-cell transcriptomes	366:410	publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	366:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	3	47	theme	cell	717:720	arg1	type					722:725	the main cell type	708:725	the main cell type contributing to this expression profile	708:765	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	47	theme	cell	717:720	arg1	fibroblasts					692:702	DP fibroblasts	689:702	DP fibroblasts	689:702	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	0	48	theme	adult	27:31	arg1	pulp					40:43	adult dental pulp	27:43	adult dental pulp	27:43	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	2	49	theme	transcriptomes	397:410	arg1	analysis					354:361	a comparative analysis	340:361	a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	340:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	50	theme	reference	449:457	arg1	tissues					459:465	healthy adult human DP to 5 other reference tissues	415:465	healthy adult human DP to 5 other reference tissues	415:465	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	7	51	link	fibroblast-derived	1291:1308	arg1	factors					1317:1323	fibroblast-derived growth factors	1291:1323	fibroblast-derived growth factors	1291:1323	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	2	52	dep	blood	479:483	arg1	cells					497:501	mononuclear cells	485:501	peripheral blood mononuclear cells	468:501	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	4	53	theme	DP	922:923	arg1	fibroblasts					925:935	DP fibroblasts	922:935	DP fibroblasts	922:935	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	6	54	theme	unappreciated	1173:1185	arg1	players					1187:1193	unappreciated players	1173:1193	unappreciated players in DP homeostasis	1173:1211	CONCLUSIONS DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells.
37024794	1	55	theme	BACKGROUND	117:126	arg1	knowledge					137:145	BACKGROUND In-depth knowledge	117:145	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP)	117:207	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	2	56	theme	other	443:447	arg1	tissues					459:465	healthy adult human DP to 5 other reference tissues	415:465	healthy adult human DP to 5 other reference tissues	415:465	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	57	theme	single-cell	385:395	arg1	marrow					509:514	bone marrow	504:514	bone marrow	504:514	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	57	theme	single-cell	385:395	arg1	skin					543:546	skin	543:546	skin	543:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	57	theme	single-cell	385:395	arg1	blood					479:483	peripheral blood mononuclear cells	468:501	peripheral blood mononuclear cells	468:501	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	57	theme	single-cell	385:395	arg1	transcriptomes					397:410	publicly available single-cell transcriptomes	366:410	publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	366:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	57	theme	single-cell	385:395	arg1	tissue					525:530	adipose tissue	517:530	adipose tissue	517:530	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	57	theme	single-cell	385:395	arg1	lung					533:536	lung	533:536	lung	533:536	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	5	58	theme	mesenchymal	1070:1080	arg1	cells					1087:1091	mesenchymal stem cells	1070:1091	mesenchymal stem cells	1070:1091	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	5	59	theme	DP	993:994	arg1	fibroblasts					996:1006	DP fibroblasts	993:1006	DP fibroblasts	993:1006	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	7	60	theme	fibroblast-derived	1291:1308	arg1	factors					1317:1323	fibroblast-derived growth factors	1291:1323	fibroblast-derived growth factors	1291:1323	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	1	61	theme	In-depth	128:135	arg1	knowledge					137:145	BACKGROUND In-depth knowledge	117:145	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP)	117:207	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	3	62	theme	DP	584:585	arg1	cells					596:600	DP resident cells	584:600	DP resident cells	584:600	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	63	theme	resident	587:594	arg1	cells					596:600	DP resident cells	584:600	DP resident cells	584:600	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	2	64	dep	transcriptomes	397:410	arg1	marrow					509:514	bone marrow	504:514	bone marrow	504:514	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	64	dep	transcriptomes	397:410	arg1	skin					543:546	skin	543:546	skin	543:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	64	dep	transcriptomes	397:410	arg1	blood					479:483	peripheral blood mononuclear cells	468:501	peripheral blood mononuclear cells	468:501	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	64	dep	transcriptomes	397:410	arg1	transcriptomes					397:410	publicly available single-cell transcriptomes	366:410	publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	366:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	64	dep	transcriptomes	397:410	arg1	tissue					525:530	adipose tissue	517:530	adipose tissue	517:530	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	2	64	dep	transcriptomes	397:410	arg1	lung					533:536	lung	533:536	lung	533:536	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	4	65	theme	heparin-binding	834:848	arg1	growth-factors					850:863	homologous heparin-binding growth-factors	823:863	homologous heparin-binding growth-factors	823:863	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	4	65	theme	heparin-binding	834:848	arg1	Genes					768:772	Genes	768:772	Genes coding for pleiotrophin (PTN) and midkine (MDK)	768:820	Genes coding for pleiotrophin (PTN) and midkine (MDK), homologous heparin-binding growth-factors, possessed the highest differential expression levels in DP fibroblasts.
37024794	3	66	theme	expression	621:630	arg1	profile					632:638	a unique gene expression profile	607:638	a unique gene expression profile	607:638	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	7	67	theme	major	1333:1337	arg1	functions					1350:1358	major regulatory functions	1333:1358	major regulatory functions	1333:1358	These findings suggest that fibroblast-derived growth factors possess major regulatory functions and thus have a potential role as dental therapeutic targets.
37024794	5	68	theme	resident	1029:1036	arg1	cells					1038:1042	several other DP resident cells	1012:1042	several other DP resident cells	1012:1042	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	2	69	theme	comparative	342:352	arg1	analysis					354:361	a comparative analysis	340:361	a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin	340:546	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	5	70	theme	several	1012:1018	arg1	cells					1038:1042	several other DP resident cells	1012:1042	several other DP resident cells	1012:1042	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	0	71	theme	heparin-binding	60:74	arg1	factors					83:89	heparin-binding growth factors Pleiotrophin and Midkine	60:114	heparin-binding growth factors Pleiotrophin and Midkine	60:114	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	0	71	theme	heparin-binding	60:74	arg1	Midkine					108:114	Midkine	108:114	Midkine	108:114	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	0	71	theme	heparin-binding	60:74	arg1	Pleiotrophin					91:102	Pleiotrophin	91:102	Pleiotrophin	91:102	Intercellular crosstalk in adult dental pulp is mediated by heparin-binding growth factors Pleiotrophin and Midkine.
37024794	5	72	theme	other	1020:1024	arg1	cells					1038:1042	several other DP resident cells	1012:1042	several other DP resident cells	1012:1042	In addition, we identified extensive crosstalk between DP fibroblasts and several other DP resident cells, including Schwann cells, mesenchymal stem cells and odontoblasts, mediated by PTN and MDK.
37024794	6	73	theme	DP	1148:1149	arg1	fibroblasts					1151:1161	CONCLUSIONS DP fibroblasts	1136:1161	CONCLUSIONS DP fibroblasts	1136:1161	CONCLUSIONS DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells.
37024794	6	74	from	players	1187:1193	arg1	homeostasis					1201:1211	DP homeostasis	1198:1211	DP homeostasis	1198:1211	CONCLUSIONS DP fibroblasts emerge as unappreciated players in DP homeostasis, mainly through their crosstalk with glial cells.
37024794	2	75	theme	mononuclear	485:495	arg1	cells					497:501	mononuclear cells	485:501	peripheral blood mononuclear cells	468:501	To address these questions, we performed a comparative analysis of publicly available single-cell transcriptomes of healthy adult human DP to 5 other reference tissues: peripheral blood mononuclear cells, bone marrow, adipose tissue, lung, and skin.
37024794	3	76	theme	reference	661:669	arg1	tissues					671:677	the reference tissues	657:677	the reference tissues	657:677	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	3	77	theme	expression	748:757	arg1	profile					759:765	this expression profile	743:765	this expression profile	743:765	RESULTS Our analysis revealed that DP resident cells have a unique gene expression profile when compared to the reference tissues, and that DP fibroblasts are the main cell type contributing to this expression profile.
37024794	1	78	theme	cellular	154:161	arg1	composition					177:187	the cellular and molecular composition	150:187	the cellular and molecular composition of dental pulp (DP)	150:207	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
37024794	1	79	theme	DP	235:236	arg1	cells					238:242	DP cells	235:242	DP cells that drive tissue homeostasis	235:272	BACKGROUND In-depth knowledge of the cellular and molecular composition of dental pulp (DP) and the crosstalk between DP cells that drive tissue homeostasis are not well understood.
36720403	3	0	theme	NAM	689:691	arg1	VU6008667					693:701	the novel, short-acting M5 NAM VU6008667	662:701	the novel, short-acting M5 NAM VU6008667	662:701	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	10	1	theme	novel	1841:1845	arg1	treatment					1865:1873	a novel, non-opioid based treatment	1839:1873	a novel, non-opioid based treatment for distinct aspects of OUD	1839:1901	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	5	2	theme	behavior	1275:1282	arg1	acquisition					1230:1240	acquisition	1230:1240	acquisition of oxycodone self-administration behavior	1230:1282	When administered daily to opioid-naïve rats, VU6008667 prevented acquisition of oxycodone self-administration behavior.
36720403	10	3	theme	distinct	1879:1886	arg1	aspects					1888:1894	distinct aspects	1879:1894	distinct aspects of OUD	1879:1901	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	1	4	theme	opioid	292:297	arg1	OUD					313:315	OUD	313:315	OUD	313:315	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	1	4	theme	opioid	292:297	arg1	disorder					303:310	opioid use disorder	292:310	opioid use disorder (OUD)	292:316	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	2	5	from	group	345:349	arg1	studies					328:334	Previous studies	319:334	Previous studies from our group and others	319:360	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	7	6	theme	self-administration	1525:1543	arg1	acquisition					1491:1501	acquisition	1491:1501	acquisition of sucrose maintained self-administration	1491:1543	After acute administration, VU6008667 did not inhibit sucrose self-administration and, when given chronically, delayed but did not prevent acquisition of sucrose maintained self-administration.
36720403	3	7	theme	reinforcing	710:720	arg1	effects					722:728	the reinforcing effects	706:728	the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats	706:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	5	8	theme	self-administration	1255:1273	arg1	behavior					1275:1282	oxycodone self-administration behavior	1245:1282	oxycodone self-administration behavior	1245:1282	When administered daily to opioid-naïve rats, VU6008667 prevented acquisition of oxycodone self-administration behavior.
36720403	1	9	theme	receptor	213:220	arg1	inhibition					167:176	inhibition	167:176	inhibition of the M5 muscarinic acetylcholine receptor (mAChR)	167:228	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	4	10	theme	Acute	876:880	arg1	VU6008667					882:890	Acute VU6008667	876:890	Acute VU6008667	876:890	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	2	11	from	others	355:360	arg1	studies					328:334	Previous studies	319:334	Previous studies from our group and others	319:360	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	7	12	theme	sucrose	1506:1512	arg1	self-administration					1525:1543	sucrose maintained self-administration	1506:1543	sucrose maintained self-administration	1506:1543	After acute administration, VU6008667 did not inhibit sucrose self-administration and, when given chronically, delayed but did not prevent acquisition of sucrose maintained self-administration.
36720403	3	13	from	effects	722:728	arg1	rats					815:818	male Sprague-Dawley rats	795:818	male Sprague-Dawley rats	795:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	6	14	theme	naloxone-precipitated	1318:1338	arg1	withdrawal					1340:1349	naloxone-precipitated withdrawal	1318:1349	naloxone-precipitated withdrawal	1318:1349	VU6008667 had minimal effects on naloxone-precipitated withdrawal.
36720403	2	15	theme	Previous	319:326	arg1	studies					328:334	Previous studies	319:334	Previous studies from our group and others	319:360	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	4	16	theme	used	1144:1147	arg1	model					1157:1161	a commonly used relapse model	1133:1161	a commonly used relapse model	1133:1161	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	4	16	theme	used	1144:1147	arg1	self-administration					1112:1130	oxycodone self-administration	1102:1130	oxycodone self-administration	1102:1130	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	3	17	from	oxycodone	733:741	arg1	rats					815:818	male Sprague-Dawley rats	795:818	male Sprague-Dawley rats	795:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	3	18	theme	male	795:798	arg1	rats					815:818	male Sprague-Dawley rats	795:818	male Sprague-Dawley rats	795:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	6	19	theme	minimal	1299:1305	arg1	effects					1307:1313	minimal effects	1299:1313	minimal effects	1299:1313	VU6008667 had minimal effects on naloxone-precipitated withdrawal.
36720403	3	20	theme	behaviors	782:790	arg1	reinstatement					747:759	reinstatement	747:759	reinstatement	747:759	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	3	20	theme	behaviors	782:790	arg1	oxycodone					733:741	oxycodone	733:741	oxycodone	733:741	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	10	21	theme	opioid	1917:1922	arg1	intake					1924:1929	opioid intake	1917:1929	opioid intake in established OUD	1917:1948	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	4	22	theme	ratio	949:953	arg1	schedules					990:998	both fixed ratio 3 (FR3) and progressive ratio (PR) schedules	938:998	both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement	938:1015	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	8	23	theme	induced	1586:1592	arg1	anti-nociception					1594:1609	oxycodone induced anti-nociception	1576:1609	oxycodone induced anti-nociception	1576:1609	VU6008667 also did not impact oxycodone induced anti-nociception or motor coordination, but mildly decreased novelty exploration.
36720403	4	24	from	self-administration	1112:1130	arg1	extinction					1086:1095	extinction	1086:1095	extinction from oxycodone self-administration, a commonly used relapse model	1086:1161	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	2	25	theme	ethanol	525:531	arg1	self-administration					493:511	established self-administration	481:511	established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats	481:568	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	10	26	theme	M5	1818:1819	arg1	mAChR					1821:1825	the M5 mAChR	1814:1825	the M5 mAChR	1814:1825	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	4	27	theme	lever	1061:1065	arg1	pressing					1067:1074	lever pressing	1061:1074	lever pressing	1061:1074	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	2	28	theme	cocaine	516:522	arg1	self-administration					493:511	established self-administration	481:511	established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats	481:568	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	3	29	theme	physiological	835:847	arg1	withdrawal					849:858	physiological withdrawal	835:858	physiological withdrawal from oxycodone	835:873	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	1	30	theme	muscarinic	188:197	arg1	mAChR					223:227	mAChR	223:227	mAChR	223:227	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	1	30	theme	muscarinic	188:197	arg1	receptor					213:220	the M5 muscarinic acetylcholine receptor	181:220	the M5 muscarinic acetylcholine receptor (mAChR)	181:228	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	4	31	theme	progressive	967:977	arg1	ratio					979:983	progressive ratio	967:983	progressive ratio (PR)	967:988	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	4	31	theme	progressive	967:977	arg1	PR					986:987	PR	986:987	PR	986:987	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	0	32	theme	opioid	109:114	arg1	self-administration					116:134	opioid self-administration	109:134	opioid self-administration	109:134	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	10	33	from	intake	1924:1929	arg1	OUD					1946:1948	established OUD	1934:1948	established OUD	1934:1948	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	2	34	theme	oxycodone	534:542	arg1	self-administration					493:511	established self-administration	481:511	established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats	481:568	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	0	35	theme	Selective	0:8	arg1	modulator					67:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator	0:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	1	36	theme	use	299:301	arg1	OUD					313:315	OUD	313:315	OUD	313:315	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	1	36	theme	use	299:301	arg1	disorder					303:310	opioid use disorder	292:310	opioid use disorder (OUD)	292:316	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	9	37	theme	VU6008667	1700:1708	arg1	administration					1710:1723	acute or daily VU6008667 administration	1685:1723	acute or daily VU6008667 administration	1685:1723	Finally, acute or daily VU6008667 administration did not impair cued fear conditioning.
36720403	4	38	theme	pressing	1067:1074	arg1	reinstatement					1044:1056	cue-induced reinstatement	1032:1056	cue-induced reinstatement of lever pressing	1032:1074	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	2	39	theme	allosteric	437:446	arg1	NAM					459:461	NAM	459:461	NAM	459:461	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	2	39	theme	allosteric	437:446	arg1	modulator					448:456	negative allosteric modulator	428:456	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	0	40	theme	muscarinic	13:22	arg1	modulator					67:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator	0:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	1	41	theme	Recent	137:142	arg1	evidence					144:151	Recent evidence	137:151	Recent evidence	137:151	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	7	42	theme	sucrose	1406:1412	arg1	self-administration					1414:1432	sucrose self-administration	1406:1432	sucrose self-administration	1406:1432	After acute administration, VU6008667 did not inhibit sucrose self-administration and, when given chronically, delayed but did not prevent acquisition of sucrose maintained self-administration.
36720403	2	43	theme	M5	425:426	arg1	ML375					464:468	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	0	44	theme	receptor	38:45	arg1	modulator					67:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator	0:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	5	45	theme	opioid-naïve	1191:1202	arg1	rats					1204:1207	opioid-naïve rats	1191:1207	opioid-naïve rats	1191:1207	When administered daily to opioid-naïve rats, VU6008667 prevented acquisition of oxycodone self-administration behavior.
36720403	2	46	theme	established	481:491	arg1	self-administration					493:511	established self-administration	481:511	established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats	481:568	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	4	47	theme	cue-induced	1032:1042	arg1	reinstatement					1044:1056	cue-induced reinstatement	1032:1056	cue-induced reinstatement of lever pressing	1032:1074	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	3	48	from	rats	815:818	arg1	effects					722:728	the reinforcing effects	706:728	the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats	706:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	3	49	theme	administration	644:657	arg1	effects					614:620	the effects	610:620	the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone	610:873	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	0	50	theme	allosteric	56:65	arg1	modulator					67:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator	0:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	9	51	theme	fear	1745:1748	arg1	conditioning					1750:1761	cued fear conditioning	1740:1761	cued fear conditioning	1740:1761	Finally, acute or daily VU6008667 administration did not impair cued fear conditioning.
36720403	3	52	theme	M5	686:687	arg1	VU6008667					693:701	the novel, short-acting M5 NAM VU6008667	662:701	the novel, short-acting M5 NAM VU6008667	662:701	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	1	53	theme	novel	244:248	arg1	mechanism					261:269	a novel non-opioid mechanism	242:269	a novel non-opioid mechanism for the treatment of opioid use disorder (OUD)	242:316	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	10	54	theme	based	1859:1863	arg1	treatment					1865:1873	a novel, non-opioid based treatment	1839:1873	a novel, non-opioid based treatment for distinct aspects of OUD	1839:1901	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	3	55	dep	novel	666:670	arg1	short-acting					673:684	short-acting	673:684	short-acting	673:684	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	4	56	theme	oxycodone	1102:1110	arg1	model					1157:1161	a commonly used relapse model	1133:1161	a commonly used relapse model	1133:1161	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	4	56	theme	oxycodone	1102:1110	arg1	self-administration					1112:1130	oxycodone self-administration	1102:1130	oxycodone self-administration	1102:1130	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	3	57	theme	VU6008667	693:701	arg1	administration					644:657	acute and repeated administration	625:657	acute and repeated administration of the novel, short-acting M5 NAM VU6008667	625:701	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	4	58	theme	oxycodone	902:910	arg1	self-administration					912:930	oxycodone self-administration	902:930	oxycodone self-administration	902:930	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	3	59	theme	oxycodone	733:741	arg1	effects					722:728	the reinforcing effects	706:728	the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats	706:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	1	60	theme	acetylcholine	199:211	arg1	mAChR					223:227	mAChR	223:227	mAChR	223:227	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	1	60	theme	acetylcholine	199:211	arg1	receptor					213:220	the M5 muscarinic acetylcholine receptor	181:220	the M5 muscarinic acetylcholine receptor (mAChR)	181:228	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	3	61	theme	present	578:584	arg1	study					586:590	the present study	574:590	the present study	574:590	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	7	62	theme	maintained	1514:1523	arg1	self-administration					1525:1543	sucrose maintained self-administration	1506:1543	sucrose maintained self-administration	1506:1543	After acute administration, VU6008667 did not inhibit sucrose self-administration and, when given chronically, delayed but did not prevent acquisition of sucrose maintained self-administration.
36720403	4	63	theme	relapse	1149:1155	arg1	model					1157:1161	a commonly used relapse model	1133:1161	a commonly used relapse model	1133:1161	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	4	63	theme	relapse	1149:1155	arg1	self-administration					1112:1130	oxycodone self-administration	1102:1130	oxycodone self-administration	1102:1130	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	3	64	theme	oxycodone-seeking	764:780	arg1	behaviors					782:790	oxycodone-seeking behaviors	764:790	oxycodone-seeking behaviors	764:790	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	10	65	theme	OUD	1899:1901	arg1	aspects					1888:1894	distinct aspects	1879:1894	distinct aspects of OUD	1879:1901	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	3	66	from	oxycodone	865:873	arg1	withdrawal					849:858	physiological withdrawal	835:858	physiological withdrawal from oxycodone	835:873	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	3	67	theme	reinstatement	747:759	arg1	effects					722:728	the reinforcing effects	706:728	the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats	706:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	10	68	theme	established	1934:1944	arg1	OUD					1946:1948	established OUD	1934:1948	established OUD	1934:1948	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	2	69	theme	remifentanil	549:560	arg1	self-administration					493:511	established self-administration	481:511	established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats	481:568	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	8	70	theme	oxycodone	1576:1584	arg1	anti-nociception					1594:1609	oxycodone induced anti-nociception	1576:1609	oxycodone induced anti-nociception	1576:1609	VU6008667 also did not impact oxycodone induced anti-nociception or motor coordination, but mildly decreased novelty exploration.
36720403	3	71	theme	Sprague-Dawley	800:813	arg1	rats					815:818	male Sprague-Dawley rats	795:818	male Sprague-Dawley rats	795:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	3	72	theme	acute	625:629	arg1	administration					644:657	acute and repeated administration	625:657	acute and repeated administration of the novel, short-acting M5 NAM VU6008667	625:701	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	4	73	theme	fixed	943:947	arg1	ratio					949:953	fixed ratio 3	943:955	fixed ratio 3 (FR3)	943:961	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	8	74	theme	motor	1614:1618	arg1	coordination					1620:1631	motor coordination	1614:1631	motor coordination	1614:1631	VU6008667 also did not impact oxycodone induced anti-nociception or motor coordination, but mildly decreased novelty exploration.
36720403	4	75	dep	ratio	949:953	arg1	FR3					958:960	FR3	958:960	FR3	958:960	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	1	76	theme	M5	185:186	arg1	mAChR					223:227	mAChR	223:227	mAChR	223:227	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	1	76	theme	M5	185:186	arg1	receptor					213:220	the M5 muscarinic acetylcholine receptor	181:220	the M5 muscarinic acetylcholine receptor (mAChR)	181:228	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	0	77	theme	self-administration	116:134	arg1	acquisition					94:104	acquisition	94:104	acquisition of opioid self-administration	94:134	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	8	78	theme	novelty	1655:1661	arg1	exploration					1663:1673	novelty exploration	1655:1673	novelty exploration	1655:1673	VU6008667 also did not impact oxycodone induced anti-nociception or motor coordination, but mildly decreased novelty exploration.
36720403	10	79	dep	novel	1841:1845	arg1	non-opioid					1848:1857	non-opioid	1848:1857	non-opioid	1848:1857	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	3	80	from	effects	614:620	arg1	effects					722:728	the reinforcing effects	706:728	the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats	706:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	3	80	from	effects	614:620	arg1	withdrawal					849:858	physiological withdrawal	835:858	physiological withdrawal from oxycodone	835:873	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	9	81	theme	acute	1685:1689	arg1	administration					1710:1723	acute or daily VU6008667 administration	1685:1723	acute or daily VU6008667 administration	1685:1723	Finally, acute or daily VU6008667 administration did not impair cued fear conditioning.
36720403	4	82	theme	ratio	979:983	arg1	schedules					990:998	both fixed ratio 3 (FR3) and progressive ratio (PR) schedules	938:998	both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement	938:1015	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	2	83	from	self-administration	493:511	arg1	rats					565:568	rats	565:568	rats	565:568	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	3	84	from	reinstatement	747:759	arg1	rats					815:818	male Sprague-Dawley rats	795:818	male Sprague-Dawley rats	795:818	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	0	85	theme	M5	10:11	arg1	modulator					67:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator	0:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	9	86	theme	daily	1694:1698	arg1	administration					1710:1723	acute or daily VU6008667 administration	1685:1723	acute or daily VU6008667 administration	1685:1723	Finally, acute or daily VU6008667 administration did not impair cued fear conditioning.
36720403	6	87	contain	had	1295:1297	arg2	effects					1307:1313	minimal effects	1299:1313	minimal effects	1299:1313	VU6008667 had minimal effects on naloxone-precipitated withdrawal.
36720403	6	87	contain	had	1295:1297	arg1	VU6008667					1285:1293	VU6008667	1285:1293	VU6008667	1285:1293	VU6008667 had minimal effects on naloxone-precipitated withdrawal.
36720403	2	88	theme	acute	385:389	arg1	administration					391:404	acute administration	385:404	acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375	385:468	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	0	89	theme	acetylcholine	24:36	arg1	modulator					67:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator	0:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	1	90	theme	disorder	303:310	arg1	treatment					279:287	the treatment	275:287	the treatment of opioid use disorder (OUD)	275:316	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	2	91	theme	modulator	448:456	arg1	ML375					464:468	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	2	92	theme	negative	428:435	arg1	NAM					459:461	NAM	459:461	NAM	459:461	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	2	92	theme	negative	428:435	arg1	modulator					448:456	negative allosteric modulator	428:456	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	0	93	theme	negative	47:54	arg1	modulator					67:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator	0:75	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	5	94	theme	oxycodone	1245:1253	arg1	behavior					1275:1282	oxycodone self-administration behavior	1245:1282	oxycodone self-administration behavior	1245:1282	When administered daily to opioid-naïve rats, VU6008667 prevented acquisition of oxycodone self-administration behavior.
36720403	4	95	theme	reinforcement	1003:1015	arg1	schedules					990:998	both fixed ratio 3 (FR3) and progressive ratio (PR) schedules	938:998	both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement	938:1015	Acute VU6008667 decreased oxycodone self-administration under both fixed ratio 3 (FR3) and progressive ratio (PR) schedules of reinforcement and attenuated cue-induced reinstatement of lever pressing following extinction from oxycodone self-administration, a commonly used relapse model.
36720403	3	96	theme	repeated	635:642	arg1	administration					644:657	acute and repeated administration	625:657	acute and repeated administration of the novel, short-acting M5 NAM VU6008667	625:701	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	2	97	theme	long-acting	413:423	arg1	ML375					464:468	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	the long-acting M5 negative allosteric modulator (NAM) ML375	409:468	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	0	98	theme	modulator	67:75	arg1	VU6008667					77:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667	0:85	Selective M5 muscarinic acetylcholine receptor negative allosteric modulator VU6008667 blocks acquisition of opioid self-administration.
36720403	9	99	theme	cued	1740:1743	arg1	conditioning					1750:1761	cued fear conditioning	1740:1761	cued fear conditioning	1740:1761	Finally, acute or daily VU6008667 administration did not impair cued fear conditioning.
36720403	2	100	theme	ML375	464:468	arg1	administration					391:404	acute administration	385:404	acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375	385:468	Previous studies from our group and others have demonstrated that acute administration of the long-acting M5 negative allosteric modulator (NAM) ML375 attenuates established self-administration of cocaine, ethanol, oxycodone, and remifentanil in rats.
36720403	7	101	theme	acute	1358:1362	arg1	administration					1364:1377	acute administration	1358:1377	acute administration	1358:1377	After acute administration, VU6008667 did not inhibit sucrose self-administration and, when given chronically, delayed but did not prevent acquisition of sucrose maintained self-administration.
36720403	3	102	theme	novel	666:670	arg1	VU6008667					693:701	the novel, short-acting M5 NAM VU6008667	662:701	the novel, short-acting M5 NAM VU6008667	662:701	In the present study, we characterized the effects of acute and repeated administration of the novel, short-acting M5 NAM VU6008667 on the reinforcing effects of oxycodone and reinstatement of oxycodone-seeking behaviors in male Sprague-Dawley rats, as well as on physiological withdrawal from oxycodone.
36720403	10	103	theme	mAChR	1821:1825	arg1	inhibition					1800:1809	inhibition	1800:1809	inhibition of the M5 mAChR	1800:1825	Overall, these results suggest that inhibition of the M5 mAChR may provide a novel, non-opioid based treatment for distinct aspects of OUD by inhibiting opioid intake in established OUD, reducing relapse during abstinence, and by reducing the risk of developing OUD.
36720403	1	104	theme	non-opioid	250:259	arg1	mechanism					261:269	a novel non-opioid mechanism	242:269	a novel non-opioid mechanism for the treatment of opioid use disorder (OUD)	242:316	Recent evidence suggests that inhibition of the M5 muscarinic acetylcholine receptor (mAChR) may provide a novel non-opioid mechanism for the treatment of opioid use disorder (OUD).
36720403	7	105	dep	inhibit	1398:1404	arg1	delayed					1463:1469	delayed	1463:1469	delayed	1463:1469	After acute administration, VU6008667 did not inhibit sucrose self-administration and, when given chronically, delayed but did not prevent acquisition of sucrose maintained self-administration.
36403513	9	0	from	effects	1198:1204	arg1	IBD					1216:1218	IBD	1216:1218	IBD	1216:1218	The gut microbiota composition was also studied to determine the mechanism for the beneficial effects of MDA on IBD.
36403513	12	1	theme	factor-2	1704:1711	arg1	pathway					1731:1737	the NF-E2-related factor-2 (Nrf2) signalling pathway	1686:1737	the NF-E2-related factor-2 (Nrf2) signalling pathway	1686:1737	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	6	2	theme	body	750:753	arg1	weight					766:771	The body and spleen weight	746:771	weight	766:771	The body and spleen weight and colon length and weight were measured and the clinical symptoms were analysed.
36403513	15	3	theme	strategies	2253:2262	arg1	development					2211:2221	the development	2207:2221	the development of preventive and therapeutic strategies for IBD	2207:2270	The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.
36403513	6	4	theme	clinical	823:830	arg1	symptoms					832:839	the clinical symptoms	819:839	the clinical symptoms	819:839	The body and spleen weight and colon length and weight were measured and the clinical symptoms were analysed.
36403513	12	5	theme	barrier	1655:1661	arg1	function					1663:1670	the intestinal barrier function	1640:1670	the intestinal barrier function	1640:1670	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	13	6	theme	DSS-induced	1911:1921	arg1	colitis					1923:1929	DSS-induced colitis	1911:1929	DSS-induced colitis mice	1911:1934	In addition, MDA administration modulates the gut microbiota composition in DSS-induced colitis mice.
36403513	11	7	theme	tumour	1456:1461	arg1	factor-alpha					1472:1483	tumour necrosis factor-alpha	1456:1483	tumour necrosis factor-alpha (TNFα)	1456:1490	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	7	theme	tumour	1456:1461	arg1	TNFα					1486:1489	TNFα	1486:1489	TNFα	1486:1489	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	7	theme	tumour	1456:1461	arg1	cytokines					1446:1454	serum inflammatory cytokines	1427:1454	serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6)	1427:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	1	8	theme	gastrointestinal	238:253	arg1	disease					295:301	inflammatory bowel disease	276:301	inflammatory bowel disease (IBD)	276:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	8	theme	gastrointestinal	238:253	arg1	disorders					255:263	gastrointestinal disorders	238:263	gastrointestinal disorders	238:263	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	5	9	theme	acute	719:723	arg1	model					739:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	METHODS The effects of MDA were evaluated using a dextran sulphate sodium (DSS)-induced acute colitis mouse model.
36403513	12	10	theme	proteins	1786:1793	arg1	expression					1757:1766	the expression	1753:1766	the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin	1753:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	10	11	theme	disease	1342:1348	arg1	index					1359:1363	disease activity index	1342:1363	disease activity index	1342:1363	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	3	12	theme	constituents	481:492	arg1	constituents					481:492	the most abundant constituents	463:492	the most abundant constituents isolated from CMG	463:510	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	3	12	theme	constituents	481:492	arg1	one					456:458	one	456:458	one	456:458	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	4	13	theme	colitis	622:628	arg1	pathogenesis					606:617	the pathogenesis	602:617	the pathogenesis of colitis	602:628	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	13	14	from	composition	1896:1906	arg1	mice					1931:1934	DSS-induced colitis mice	1911:1934	DSS-induced colitis mice	1911:1934	In addition, MDA administration modulates the gut microbiota composition in DSS-induced colitis mice.
36403513	8	15	theme	Colon	937:941	arg1	tissues					943:949	Colon tissues	937:949	Colon tissues	937:949	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	9	16	theme	microbiota	1112:1121	arg1	composition					1123:1133	The gut microbiota composition	1104:1133	The gut microbiota composition	1104:1133	The gut microbiota composition was also studied to determine the mechanism for the beneficial effects of MDA on IBD.
36403513	14	17	theme	gut	2095:2097	arg1	microbiota					2099:2108	the gut microbiota	2091:2108	the gut microbiota	2091:2108	CONCLUSION The results indicate that MDA attenuated experimental colitis by restoring intestinal barrier integrity, reducing inflammation, and modulating the gut microbiota.
36403513	5	18	theme	MDA	654:656	arg1	effects					643:649	The effects	639:649	METHODS The effects of MDA	631:656	METHODS The effects of MDA were evaluated using a dextran sulphate sodium (DSS)-induced acute colitis mouse model.
36403513	1	19	theme	Greek	196:200	arg1	medicine					202:209	a traditional Greek medicine	182:209	a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD)	182:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	19	theme	Greek	196:200	arg1	gum					169:171	BACKGROUND Chios mastic gum	145:171	BACKGROUND Chios mastic gum (CMG)	145:177	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	12	20	theme	tight	1771:1775	arg1	occludin					1825:1832	occludin	1825:1832	occludin	1825:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	20	theme	tight	1771:1775	arg1	occludens-1					1802:1812	zonula occludens-1	1795:1812	zonula occludens-1 (ZO-1)	1795:1819	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	20	theme	tight	1771:1775	arg1	proteins					1786:1793	tight junction proteins	1771:1793	tight junction proteins zonula occludens-1 (ZO-1) and occludin	1771:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	7	21	theme	markers	928:934	arg1	level					900:904	the level	896:904	the level of serum inflammatory markers	896:934	Blood samples were collected to analyse the level of serum inflammatory markers.
36403513	12	22	theme	MDA	1601:1603	arg1	supplementation					1605:1619	MDA supplementation	1601:1619	MDA supplementation	1601:1619	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	9	23	theme	MDA	1209:1211	arg1	effects					1198:1204	the beneficial effects	1183:1204	the beneficial effects of MDA on IBD	1183:1218	The gut microbiota composition was also studied to determine the mechanism for the beneficial effects of MDA on IBD.
36403513	7	24	theme	serum	909:913	arg1	markers					928:934	serum inflammatory markers	909:934	serum inflammatory markers	909:934	Blood samples were collected to analyse the level of serum inflammatory markers.
36403513	1	25	theme	BACKGROUND	145:154	arg1	CMG					174:176	CMG	174:176	CMG	174:176	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	25	theme	BACKGROUND	145:154	arg1	gum					169:171	BACKGROUND Chios mastic gum	145:171	BACKGROUND Chios mastic gum (CMG)	145:177	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	25	theme	BACKGROUND	145:154	arg1	medicine					202:209	a traditional Greek medicine	182:209	a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD)	182:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	8	26	theme	barrier	1030:1036	arg1	function					1038:1045	the epithelial barrier function	1015:1045	the epithelial barrier function	1015:1045	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	11	27	theme	cytokines	1446:1454	arg1	release					1416:1422	the release	1412:1422	the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6)	1412:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	4	28	dep	effects	558:564	arg1	the					544:546	the	544:546	the	544:546	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	1	29	theme	mastic	162:167	arg1	CMG					174:176	CMG	174:176	CMG	174:176	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	29	theme	mastic	162:167	arg1	gum					169:171	BACKGROUND Chios mastic gum	145:171	BACKGROUND Chios mastic gum (CMG)	145:177	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	29	theme	mastic	162:167	arg1	medicine					202:209	a traditional Greek medicine	182:209	a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD)	182:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	11	30	theme	serum	1427:1431	arg1	factor-alpha					1472:1483	tumour necrosis factor-alpha	1456:1483	tumour necrosis factor-alpha (TNFα)	1456:1490	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	30	theme	serum	1427:1431	arg1	interleukin					1525:1535	interleukin 6	1525:1537	interleukin 6 (IL-6)	1525:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	30	theme	serum	1427:1431	arg1	beta					1507:1510	interleukin 1 beta	1493:1510	interleukin 1 beta (IL-1β)	1493:1518	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	30	theme	serum	1427:1431	arg1	cytokines					1446:1454	serum inflammatory cytokines	1427:1454	serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6)	1427:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	0	31	theme	inflammatory	84:95	arg1	response					97:104	inflammatory response	84:104	inflammatory response	84:104	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	14	32	theme	barrier	2034:2040	arg1	integrity					2042:2050	intestinal barrier integrity	2023:2050	intestinal barrier integrity	2023:2050	CONCLUSION The results indicate that MDA attenuated experimental colitis by restoring intestinal barrier integrity, reducing inflammation, and modulating the gut microbiota.
36403513	10	33	theme	RESULTS	1221:1227	arg1	MDA					1229:1231	RESULTS MDA	1221:1231	RESULTS MDA	1221:1231	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	12	34	theme	zonula	1795:1800	arg1	ZO-1					1815:1818	ZO-1	1815:1818	ZO-1	1815:1818	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	34	theme	zonula	1795:1800	arg1	occludens-1					1802:1812	zonula occludens-1	1795:1812	zonula occludens-1 (ZO-1)	1795:1819	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	34	theme	zonula	1795:1800	arg1	proteins					1786:1793	tight junction proteins	1771:1793	tight junction proteins zonula occludens-1 (ZO-1) and occludin	1771:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	0	35	theme	Masticadienonic	0:14	arg1	acid					16:19	Masticadienonic acid	0:19	Masticadienonic acid from Chios mastic gum	0:41	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	1	36	theme	disorders	255:263	arg1	variety					227:233	a variety	225:233	a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD)	225:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	36	theme	disorders	255:263	arg1	disorders					255:263	gastrointestinal disorders	238:263	gastrointestinal disorders	238:263	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	36	theme	disorders	255:263	arg1	disease					295:301	inflammatory bowel disease	276:301	inflammatory bowel disease (IBD)	276:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	11	37	dep	cytokines	1446:1454	arg1	IL-1β					1513:1517	IL-1β	1513:1517	IL-1β	1513:1517	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	37	dep	cytokines	1446:1454	arg1	TNFα					1486:1489	TNFα	1486:1489	TNFα	1486:1489	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	37	dep	cytokines	1446:1454	arg1	cytokines					1446:1454	serum inflammatory cytokines	1427:1454	serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6)	1427:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	37	dep	cytokines	1446:1454	arg1	IL-6					1540:1543	IL-6	1540:1543	IL-6	1540:1543	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	37	dep	cytokines	1446:1454	arg1	interleukin					1525:1535	interleukin 6	1525:1537	interleukin 6 (IL-6)	1525:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	37	dep	cytokines	1446:1454	arg1	factor-alpha					1472:1483	tumour necrosis factor-alpha	1456:1483	tumour necrosis factor-alpha (TNFα)	1456:1490	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	37	dep	cytokines	1446:1454	arg1	beta					1507:1510	interleukin 1 beta	1493:1510	interleukin 1 beta (IL-1β)	1493:1518	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	38	theme	MDA	1390:1392	arg1	treatments					1394:1403	MDA treatments	1390:1403	MDA treatments	1390:1403	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	3	39	theme	Masticadienonic	426:440	arg1	MDA					448:450	MDA	448:450	MDA	448:450	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	3	39	theme	Masticadienonic	426:440	arg1	acid					442:445	PURPOSE Masticadienonic acid	418:445	PURPOSE Masticadienonic acid (MDA)	418:451	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	10	40	theme	reducing	1318:1325	arg1	weight					1334:1339	reducing spleen weight	1318:1339	reducing spleen weight	1318:1339	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	2	41	theme	action	372:377	arg1	mechanisms					358:367	the mechanisms	354:367	the mechanisms of action	354:377	However, the bioactive compounds of CMG and the mechanisms of action for controlling of IBD remain unknown.
36403513	2	41	theme	action	372:377	arg1	compounds					333:341	the bioactive compounds	319:341	the bioactive compounds of CMG	319:348	However, the bioactive compounds of CMG and the mechanisms of action for controlling of IBD remain unknown.
36403513	0	42	theme	mastic	32:37	arg1	gum					39:41	Chios mastic gum	26:41	Chios mastic gum	26:41	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	1	43	theme	bowel	289:293	arg1	disease					295:301	inflammatory bowel disease	276:301	inflammatory bowel disease (IBD)	276:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	43	theme	bowel	289:293	arg1	IBD					304:306	IBD	304:306	IBD	304:306	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	12	44	theme	junction	1777:1784	arg1	occludin					1825:1832	occludin	1825:1832	occludin	1825:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	44	theme	junction	1777:1784	arg1	occludens-1					1802:1812	zonula occludens-1	1795:1812	zonula occludens-1 (ZO-1)	1795:1819	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	44	theme	junction	1777:1784	arg1	proteins					1786:1793	tight junction proteins	1771:1793	tight junction proteins zonula occludens-1 (ZO-1) and occludin	1771:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	11	45	theme	NF-κB	1574:1578	arg1	pathways					1591:1598	NF-κB signalling pathways	1574:1598	NF-κB signalling pathways	1574:1598	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	5	46	theme	mouse	733:737	arg1	model					739:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	METHODS The effects of MDA were evaluated using a dextran sulphate sodium (DSS)-induced acute colitis mouse model.
36403513	13	47	theme	gut	1881:1883	arg1	composition					1896:1906	the gut microbiota composition	1877:1906	the gut microbiota composition in DSS-induced colitis mice	1877:1934	In addition, MDA administration modulates the gut microbiota composition in DSS-induced colitis mice.
36403513	6	48	theme	spleen	759:764	arg1	weight					766:771	The body and spleen weight	746:771	weight	766:771	The body and spleen weight and colon length and weight were measured and the clinical symptoms were analysed.
36403513	12	49	theme	NF-E2-related	1690:1702	arg1	factor-2					1704:1711	NF-E2-related factor-2	1690:1711	the NF-E2-related factor-2 (Nrf2) signalling pathway	1686:1737	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	49	theme	NF-E2-related	1690:1702	arg1	Nrf2					1714:1717	Nrf2	1714:1717	Nrf2	1714:1717	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	15	50	theme	novel	2138:2142	arg1	insights					2144:2151	novel insights	2138:2151	novel insights into CMG-mediated remission of IBD	2138:2186	The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.
36403513	4	51	theme	potential	548:556	arg1	effects					558:564	potential effects	548:564	potential effects	548:564	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	9	52	theme	gut	1108:1110	arg1	composition					1123:1133	The gut microbiota composition	1104:1133	The gut microbiota composition	1104:1133	The gut microbiota composition was also studied to determine the mechanism for the beneficial effects of MDA on IBD.
36403513	2	53	theme	bioactive	323:331	arg1	compounds					333:341	the bioactive compounds	319:341	the bioactive compounds of CMG	319:348	However, the bioactive compounds of CMG and the mechanisms of action for controlling of IBD remain unknown.
36403513	15	54	theme	therapeutic	2241:2251	arg1	strategies					2253:2262	preventive and therapeutic strategies	2226:2262	preventive and therapeutic strategies for IBD	2226:2270	The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.
36403513	8	55	theme	mechanisms	1082:1091	arg1	investigation					1052:1064	investigation	1052:1064	investigation of the probable mechanisms of action	1052:1101	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	8	55	theme	mechanisms	1082:1091	arg1	evaluation					1001:1010	evaluation	1001:1010	evaluation of the epithelial barrier function	1001:1045	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	8	55	theme	mechanisms	1082:1091	arg1	examination					988:998	histopathological examination	970:998	histopathological examination	970:998	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	12	56	dep	proteins	1786:1793	arg1	ZO-1					1815:1818	ZO-1	1815:1818	ZO-1	1815:1818	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	56	dep	proteins	1786:1793	arg1	occludin					1825:1832	occludin	1825:1832	occludin	1825:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	56	dep	proteins	1786:1793	arg1	occludens-1					1802:1812	zonula occludens-1	1795:1812	zonula occludens-1 (ZO-1)	1795:1819	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	12	56	dep	proteins	1786:1793	arg1	proteins					1786:1793	tight junction proteins	1771:1793	tight junction proteins zonula occludens-1 (ZO-1) and occludin	1771:1832	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	15	57	theme	preventive	2226:2235	arg1	strategies					2253:2262	preventive and therapeutic strategies	2226:2262	preventive and therapeutic strategies for IBD	2226:2270	The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.
36403513	8	58	theme	action	1096:1101	arg1	mechanisms					1082:1091	the probable mechanisms	1069:1091	the probable mechanisms of action	1069:1101	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	15	59	theme	present	2115:2121	arg1	study					2123:2127	The present study	2111:2127	The present study	2111:2127	The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.
36403513	10	60	theme	colon	1304:1308	arg1	length					1310:1315	colon length	1304:1315	colon length	1304:1315	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	3	61	theme	abundant	472:479	arg1	constituents					481:492	the most abundant constituents	463:492	the most abundant constituents isolated from CMG	463:510	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	11	62	theme	interleukin	1493:1503	arg1	IL-1β					1513:1517	IL-1β	1513:1517	IL-1β	1513:1517	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	62	theme	interleukin	1493:1503	arg1	beta					1507:1510	interleukin 1 beta	1493:1510	interleukin 1 beta (IL-1β)	1493:1518	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	62	theme	interleukin	1493:1503	arg1	cytokines					1446:1454	serum inflammatory cytokines	1427:1454	serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6)	1427:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	12	63	theme	intestinal	1644:1653	arg1	function					1663:1670	the intestinal barrier function	1640:1670	the intestinal barrier function	1640:1670	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	10	64	theme	spleen	1327:1332	arg1	weight					1334:1339	reducing spleen weight	1318:1339	reducing spleen weight	1318:1339	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	14	65	dep	CONCLUSION	1937:1946	arg1	indicate					1960:1967	indicate	1960:1967	indicate that MDA attenuated experimental colitis by restoring intestinal barrier integrity, reducing inflammation, and modulating the gut microbiota	1960:2108	CONCLUSION The results indicate that MDA attenuated experimental colitis by restoring intestinal barrier integrity, reducing inflammation, and modulating the gut microbiota.
36403513	4	66	from	mechanisms	581:590	arg1	pathogenesis					606:617	the pathogenesis	602:617	the pathogenesis of colitis	602:628	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	1	67	theme	traditional	184:194	arg1	medicine					202:209	a traditional Greek medicine	182:209	a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD)	182:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	67	theme	traditional	184:194	arg1	gum					169:171	BACKGROUND Chios mastic gum	145:171	BACKGROUND Chios mastic gum (CMG)	145:177	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	4	68	theme	MDA	595:597	arg1	effects					558:564	potential effects	548:564	potential effects	548:564	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	4	68	theme	MDA	595:597	arg1	mechanisms					581:590	underlying mechanisms	570:590	underlying mechanisms	570:590	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	9	69	theme	beneficial	1187:1196	arg1	effects					1198:1204	the beneficial effects	1183:1204	the beneficial effects of MDA on IBD	1183:1218	The gut microbiota composition was also studied to determine the mechanism for the beneficial effects of MDA on IBD.
36403513	15	70	theme	CMG-mediated	2158:2169	arg1	remission					2171:2179	CMG-mediated remission	2158:2179	CMG-mediated remission of IBD	2158:2186	The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.
36403513	13	71	theme	MDA	1848:1850	arg1	administration					1852:1865	MDA administration	1848:1865	MDA administration	1848:1865	In addition, MDA administration modulates the gut microbiota composition in DSS-induced colitis mice.
36403513	7	72	theme	inflammatory	915:926	arg1	markers					928:934	serum inflammatory markers	909:934	serum inflammatory markers	909:934	Blood samples were collected to analyse the level of serum inflammatory markers.
36403513	11	73	theme	inflammatory	1433:1444	arg1	factor-alpha					1472:1483	tumour necrosis factor-alpha	1456:1483	tumour necrosis factor-alpha (TNFα)	1456:1490	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	73	theme	inflammatory	1433:1444	arg1	interleukin					1525:1535	interleukin 6	1525:1537	interleukin 6 (IL-6)	1525:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	73	theme	inflammatory	1433:1444	arg1	beta					1507:1510	interleukin 1 beta	1493:1510	interleukin 1 beta (IL-1β)	1493:1518	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	73	theme	inflammatory	1433:1444	arg1	cytokines					1446:1454	serum inflammatory cytokines	1427:1454	serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6)	1427:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	4	74	from	effects	558:564	arg1	pathogenesis					606:617	the pathogenesis	602:617	the pathogenesis of colitis	602:628	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	1	75	theme	Chios	156:160	arg1	CMG					174:176	CMG	174:176	CMG	174:176	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	75	theme	Chios	156:160	arg1	gum					169:171	BACKGROUND Chios mastic gum	145:171	BACKGROUND Chios mastic gum (CMG)	145:177	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	75	theme	Chios	156:160	arg1	medicine					202:209	a traditional Greek medicine	182:209	a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD)	182:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	0	76	theme	barrier	111:117	arg1	integrity					119:127	gut barrier integrity	107:127	gut barrier integrity	107:127	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	8	77	theme	histopathological	970:986	arg1	examination					988:998	histopathological examination	970:998	histopathological examination	970:998	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	0	78	from	colitis	53:59	arg1	mice					64:67	mice	64:67	mice	64:67	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	10	79	theme	activity	1350:1357	arg1	index					1359:1363	disease activity index	1342:1363	disease activity index	1342:1363	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	0	80	theme	gut	107:109	arg1	integrity					119:127	gut barrier integrity	107:127	gut barrier integrity	107:127	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	5	81	dep	METHODS	631:637	arg1	effects					643:649	The effects	639:649	METHODS The effects of MDA	631:656	METHODS The effects of MDA were evaluated using a dextran sulphate sodium (DSS)-induced acute colitis mouse model.
36403513	0	82	from	gum	39:41	arg1	acid					16:19	Masticadienonic acid	0:19	Masticadienonic acid from Chios mastic gum	0:41	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	14	83	theme	intestinal	2023:2032	arg1	integrity					2042:2050	intestinal barrier integrity	2023:2050	intestinal barrier integrity	2023:2050	CONCLUSION The results indicate that MDA attenuated experimental colitis by restoring intestinal barrier integrity, reducing inflammation, and modulating the gut microbiota.
36403513	10	84	theme	histological	1370:1381	arg1	score					1383:1387	histological score	1370:1387	histological score	1370:1387	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	2	85	theme	CMG	346:348	arg1	mechanisms					358:367	the mechanisms	354:367	the mechanisms of action	354:377	However, the bioactive compounds of CMG and the mechanisms of action for controlling of IBD remain unknown.
36403513	2	85	theme	CMG	346:348	arg1	compounds					333:341	the bioactive compounds	319:341	the bioactive compounds of CMG	319:348	However, the bioactive compounds of CMG and the mechanisms of action for controlling of IBD remain unknown.
36403513	0	86	theme	Chios	26:30	arg1	gum					39:41	Chios mastic gum	26:41	Chios mastic gum	26:41	Masticadienonic acid from Chios mastic gum mitigates colitis in mice via modulating inflammatory response, gut barrier integrity and microbiota.
36403513	8	87	theme	epithelial	1019:1028	arg1	function					1038:1045	the epithelial barrier function	1015:1045	the epithelial barrier function	1015:1045	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	7	88	theme	Blood	856:860	arg1	samples					862:868	Blood samples	856:868	Blood samples	856:868	Blood samples were collected to analyse the level of serum inflammatory markers.
36403513	3	89	attach	isolated	494:501	arg1	CMG					508:510	CMG	508:510	CMG	508:510	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	3	89	attach	isolated	494:501	arg2	constituents					481:492	the most abundant constituents	463:492	the most abundant constituents isolated from CMG	463:510	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	14	90	theme	experimental	1989:2000	arg1	colitis					2002:2008	experimental colitis	1989:2008	experimental colitis	1989:2008	CONCLUSION The results indicate that MDA attenuated experimental colitis by restoring intestinal barrier integrity, reducing inflammation, and modulating the gut microbiota.
36403513	11	91	theme	signalling	1580:1589	arg1	pathways					1591:1598	NF-κB signalling pathways	1574:1598	NF-κB signalling pathways	1574:1598	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	5	92	theme	-induced	710:717	arg1	model					739:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	METHODS The effects of MDA were evaluated using a dextran sulphate sodium (DSS)-induced acute colitis mouse model.
36403513	1	93	theme	inflammatory	276:287	arg1	disease					295:301	inflammatory bowel disease	276:301	inflammatory bowel disease (IBD)	276:307	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	1	93	theme	inflammatory	276:287	arg1	IBD					304:306	IBD	304:306	IBD	304:306	BACKGROUND Chios mastic gum (CMG) is a traditional Greek medicine used to treat a variety of gastrointestinal disorders, including inflammatory bowel disease (IBD).
36403513	8	94	theme	function	1038:1045	arg1	investigation					1052:1064	investigation	1052:1064	investigation of the probable mechanisms of action	1052:1101	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	8	94	theme	function	1038:1045	arg1	evaluation					1001:1010	evaluation	1001:1010	evaluation of the epithelial barrier function	1001:1045	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	8	94	theme	function	1038:1045	arg1	examination					988:998	histopathological examination	970:998	histopathological examination	970:998	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	13	95	theme	colitis	1923:1929	arg1	mice					1931:1934	DSS-induced colitis mice	1911:1934	DSS-induced colitis mice	1911:1934	In addition, MDA administration modulates the gut microbiota composition in DSS-induced colitis mice.
36403513	3	96	theme	PURPOSE	418:424	arg1	MDA					448:450	MDA	448:450	MDA	448:450	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	3	96	theme	PURPOSE	418:424	arg1	acid					442:445	PURPOSE Masticadienonic acid	418:445	PURPOSE Masticadienonic acid (MDA)	418:451	PURPOSE Masticadienonic acid (MDA) is one of the most abundant constituents isolated from CMG.
36403513	5	97	theme	colitis	725:731	arg1	model					739:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	a dextran sulphate sodium (DSS)-induced acute colitis mouse model	679:743	METHODS The effects of MDA were evaluated using a dextran sulphate sodium (DSS)-induced acute colitis mouse model.
36403513	6	98	theme	colon	777:781	arg1	length					783:788	colon length	777:788	colon length	777:788	The body and spleen weight and colon length and weight were measured and the clinical symptoms were analysed.
36403513	10	99	theme	IBD	1266:1268	arg1	severity					1254:1261	the severity	1250:1261	the severity of IBD	1250:1268	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	15	100	theme	IBD	2184:2186	arg1	remission					2171:2179	CMG-mediated remission	2158:2179	CMG-mediated remission of IBD	2158:2186	The present study provides novel insights into CMG-mediated remission of IBD and may facilitate the development of preventive and therapeutic strategies for IBD.
36403513	12	101	theme	signalling	1720:1729	arg1	pathway					1731:1737	the NF-E2-related factor-2 (Nrf2) signalling pathway	1686:1737	the NF-E2-related factor-2 (Nrf2) signalling pathway	1686:1737	MDA supplementation could also improve the intestinal barrier function by activating the NF-E2-related factor-2 (Nrf2) signalling pathway and restoring the expression of tight junction proteins zonula occludens-1 (ZO-1) and occludin.
36403513	13	102	theme	microbiota	1885:1894	arg1	composition					1896:1906	the gut microbiota composition	1877:1906	the gut microbiota composition in DSS-induced colitis mice	1877:1934	In addition, MDA administration modulates the gut microbiota composition in DSS-induced colitis mice.
36403513	8	103	theme	probable	1073:1080	arg1	mechanisms					1082:1091	the probable mechanisms	1069:1091	the probable mechanisms of action	1069:1101	Colon tissues were processed for histopathological examination, evaluation of the epithelial barrier function, and investigation of the probable mechanisms of action.
36403513	4	104	theme	underlying	570:579	arg1	mechanisms					581:590	underlying mechanisms	570:590	underlying mechanisms	570:590	This study aims to investigate the potential effects and underlying mechanisms of MDA in the pathogenesis of colitis.
36403513	10	105	theme	body	1288:1291	arg1	weight					1293:1298	the body weight	1284:1298	the body weight	1284:1298	RESULTS MDA could ameliorate the severity of IBD by increasing the body weight and colon length, reducing spleen weight, disease activity index, and histological score.
36403513	11	106	theme	necrosis	1463:1470	arg1	factor-alpha					1472:1483	tumour necrosis factor-alpha	1456:1483	tumour necrosis factor-alpha (TNFα)	1456:1490	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	106	theme	necrosis	1463:1470	arg1	TNFα					1486:1489	TNFα	1486:1489	TNFα	1486:1489	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
36403513	11	106	theme	necrosis	1463:1470	arg1	cytokines					1446:1454	serum inflammatory cytokines	1427:1454	serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6)	1427:1544	MDA treatments reduce the release of serum inflammatory cytokines tumour necrosis factor-alpha (TNFα), interleukin 1 beta (IL-1β), and interleukin 6 (IL-6) via inhibiting the MAPK and NF-κB signalling pathways.
35964941	7	0	theme	hydrogel	1488:1495	arg1	network					1504:1510	the hydrogel matrix network	1484:1510	the hydrogel matrix network	1484:1510	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	11	1	theme	ChsMA	2071:2075	arg1	Lipo					2077:2080	ChsMA@Lipo	2071:2080	ChsMA@Lipo	2071:2080	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	10	2	theme	liposome	1891:1898	arg1	carriers					1853:1860	the composite carriers	1839:1860	the composite carriers of hydrogel microspheres and liposome	1839:1898	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	6	3	theme	dual	1287:1290	arg1	antioxidation					1292:1304	dual antioxidation	1287:1304	dual antioxidation	1287:1304	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	9	4	theme	@	1646:1646	arg1	option					1729:1734	a promising option	1717:1734	a promising option for osteoarthritis treatment	1717:1763	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	9	4	theme	@	1646:1646	arg1	Lipo					1647:1650	ChsMA@Lipo	1641:1650	ChsMA@Lipo	1641:1650	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	1	5	theme	Reactive	134:141	arg1	ROS					159:161	ROS	159:161	ROS	159:161	Reactive oxygen species (ROS) play a critical role in the pathogenesis of osteoarthritis.
35964941	1	5	theme	Reactive	134:141	arg1	species					150:156	Reactive oxygen species	134:156	Reactive oxygen species (ROS)	134:162	Reactive oxygen species (ROS) play a critical role in the pathogenesis of osteoarthritis.
35964941	10	6	theme	different	1933:1941	arg1	materials					1943:1951	different materials	1933:1951	different materials	1933:1951	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	6	7	theme	osteoarthritis	1269:1282	arg1	progression					1254:1264	the progression	1250:1264	the progression of osteoarthritis	1250:1282	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	3	8	theme	delivery	494:501	arg1	microspheres					534:545	microspheres	534:545	microspheres	534:545	Currently, the drug circulation half-life can be extended using delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases.
35964941	3	8	theme	delivery	494:501	arg1	vehicles					503:510	delivery vehicles	494:510	delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases	494:586	Currently, the drug circulation half-life can be extended using delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases.
35964941	3	8	theme	delivery	494:501	arg1	liposomes					520:528	liposomes	520:528	liposomes	520:528	Currently, the drug circulation half-life can be extended using delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases.
35964941	7	9	theme	lipid	1465:1469	arg1	membrane					1471:1478	the lipid membrane	1461:1478	the lipid membrane	1461:1478	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	4	10	theme	drug	837:840	arg1	systems					851:857	new local drug delivery systems	827:857	new local drug delivery systems	827:857	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	4	11	theme	hydrogel	642:649	arg1	microspheres					651:662	hydrogel microspheres	642:662	hydrogel microspheres	642:662	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	2	12	theme	drug	262:265	arg1	injection					228:236	The injection	224:236	The injection of a single antioxidant drug	224:265	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	5	13	theme	Chondroitin	860:870	arg1	glycosaminoglycan					892:908	a sulfated glycosaminoglycan	881:908	a sulfated glycosaminoglycan commonly found in native cartilage	881:943	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	13	theme	Chondroitin	860:870	arg1	sulfate					872:878	Chondroitin sulfate	860:878	Chondroitin sulfate	860:878	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	11	14	theme	IL-1β-induced	2102:2114	arg1	degradation					2120:2130	IL-1β-induced ECM degradation	2102:2130	IL-1β-induced ECM degradation in chondrocytes	2102:2146	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	7	15	theme	sustained-release	1401:1417	arg1	kinetics					1419:1426	significant sustained-release kinetics	1389:1426	significant sustained-release kinetics	1389:1426	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	11	16	from	degradation	2120:2130	arg1	chondrocytes					2135:2146	chondrocytes	2135:2146	chondrocytes	2135:2146	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	0	17	theme	osteoarthritis	118:131	arg1	treatment					105:113	the treatment	101:113	the treatment of osteoarthritis	101:131	Chondroitin sulfate microspheres anchored with drug-loaded liposomes play a dual antioxidant role in the treatment of osteoarthritis.
35964941	2	18	theme	low	287:289	arg1	utilization					296:306	low drug utilization	287:306	low drug utilization	287:306	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	9	19	theme	antioxidant	1678:1688	arg1	platform					1704:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	7	20	theme	Lipo	1365:1368	arg1	microgels					1370:1378	ChsMA@Lipo microgels	1359:1378	ChsMA@Lipo microgels	1359:1378	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	4	21	theme	liposomes	628:636	arg1	carriers					616:623	the composite carriers	602:623	the composite carriers of liposomes and hydrogel microspheres	602:662	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	5	22	theme	methacryloyl	1113:1124	arg1	ChsMA					1134:1138	ChsMA	1134:1138	ChsMA	1134:1138	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	22	theme	methacryloyl	1113:1124	arg1	groups					1126:1131	photo-cross-linkable methacryloyl groups	1092:1131	photo-cross-linkable methacryloyl groups (ChsMA)	1092:1139	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	9	23	theme	delivery	1695:1702	arg1	platform					1704:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	12	24	theme	osteoarthritis	2332:2345	arg1	treatment					2347:2355	osteoarthritis treatment	2332:2355	osteoarthritis treatment	2332:2355	The obtained ChsMA@Lipo alleviates the progression of osteoarthritis in vivo, which is promising for osteoarthritis treatment.
35964941	7	25	theme	ChsMA	1359:1363	arg1	microgels					1370:1378	ChsMA@Lipo microgels	1359:1378	ChsMA@Lipo microgels	1359:1378	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	4	26	theme	material	704:711	arg1	forms					713:717	different material forms	694:717	different material forms	694:717	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	10	27	theme	single	1823:1828	arg1	carrier					1830:1836	the traditional single carrier	1807:1836	the traditional single carrier	1807:1836	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	5	28	theme	covalent	1065:1072	arg1	modification					1074:1085	covalent modification	1065:1085	covalent modification with photo-cross-linkable methacryloyl groups (ChsMA)	1065:1139	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	2	29	theme	single	243:248	arg1	drug					262:265	a single antioxidant drug	241:265	a single antioxidant drug	241:265	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	1	30	theme	oxygen	143:148	arg1	ROS					159:161	ROS	159:161	ROS	159:161	Reactive oxygen species (ROS) play a critical role in the pathogenesis of osteoarthritis.
35964941	1	30	theme	oxygen	143:148	arg1	species					150:156	Reactive oxygen species	134:156	Reactive oxygen species (ROS)	134:162	Reactive oxygen species (ROS) play a critical role in the pathogenesis of osteoarthritis.
35964941	4	31	dep	stronger	728:735	arg1	plasticity					737:746	plasticity	737:746	plasticity	737:746	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	4	31	dep	stronger	728:735	arg1	flexibility					752:762	flexibility	752:762	flexibility	752:762	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	11	32	theme	inflammasome	2206:2217	arg1	activation					2219:2228	the inflammasome activation	2202:2228	the inflammasome activation	2202:2228	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	11	32	theme	inflammasome	2206:2217	arg1	macrophages					2173:2183	the M1 macrophages polarization and the inflammasome activation	2166:2228	the M1 macrophages polarization and the inflammasome activation	2166:2228	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	7	33	theme	drug	1340:1343	arg1	LQ					1345:1346	the antioxidant drug LQ	1324:1346	the antioxidant drug LQ wrapped in ChsMA@Lipo microgels	1324:1378	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	5	34	theme	sulfated	883:890	arg1	glycosaminoglycan					892:908	a sulfated glycosaminoglycan	881:908	a sulfated glycosaminoglycan commonly found in native cartilage	881:943	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	34	theme	sulfated	883:890	arg1	sulfate					872:878	Chondroitin sulfate	860:878	Chondroitin sulfate	860:878	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	10	35	theme	new	2031:2033	arg1	system					2063:2068	a new and efficient drug delivery system	2029:2068	a new and efficient drug delivery system	2029:2068	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	0	36	theme	antioxidant	81:91	arg1	role					93:96	a dual antioxidant role	74:96	a dual antioxidant role	74:96	Chondroitin sulfate microspheres anchored with drug-loaded liposomes play a dual antioxidant role in the treatment of osteoarthritis.
35964941	9	37	theme	osteoarthritis	1740:1753	arg1	treatment					1755:1763	osteoarthritis treatment	1740:1763	osteoarthritis treatment	1740:1763	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	10	38	theme	traditional	1811:1821	arg1	carrier					1830:1836	the traditional single carrier	1807:1836	the traditional single carrier	1807:1836	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	6	39	theme	-loaded	1195:1201	arg1	Lipo					1220:1223	ChsMA@Lipo	1214:1223	ChsMA@Lipo	1214:1223	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	6	39	theme	-loaded	1195:1201	arg1	liposomes					1203:1211	liquiritin (LQ)-loaded liposomes	1180:1211	liquiritin (LQ)-loaded liposomes (ChsMA@Lipo)	1180:1224	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	12	40	theme	@	2249:2249	arg1	Lipo					2250:2253	The obtained ChsMA@Lipo	2231:2253	The obtained ChsMA@Lipo	2231:2253	The obtained ChsMA@Lipo alleviates the progression of osteoarthritis in vivo, which is promising for osteoarthritis treatment.
35964941	4	41	theme	single	781:786	arg1	carriers					788:795	traditional single carriers	769:795	traditional single carriers	769:795	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	5	42	with	modification	1074:1085	arg1	ChsMA					1134:1138	ChsMA	1134:1138	ChsMA	1134:1138	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	42	with	modification	1074:1085	arg1	groups					1126:1131	photo-cross-linkable methacryloyl groups	1092:1131	photo-cross-linkable methacryloyl groups (ChsMA)	1092:1139	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	7	43	theme	antioxidant	1328:1338	arg1	LQ					1345:1346	the antioxidant drug LQ	1324:1346	the antioxidant drug LQ wrapped in ChsMA@Lipo microgels	1324:1378	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	11	44	dep	macrophages	2173:2183	arg1	activation					2219:2228	the inflammasome activation	2202:2228	the inflammasome activation	2202:2228	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	11	44	dep	macrophages	2173:2183	arg1	macrophages					2173:2183	the M1 macrophages polarization and the inflammasome activation	2166:2228	the M1 macrophages polarization and the inflammasome activation	2166:2228	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	11	44	dep	macrophages	2173:2183	arg1	polarization					2185:2196	polarization	2185:2196	polarization	2185:2196	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	0	45	theme	Chondroitin	0:10	arg1	microspheres					20:31	Chondroitin sulfate microspheres	0:31	Chondroitin sulfate microspheres anchored with drug-loaded liposomes	0:67	Chondroitin sulfate microspheres anchored with drug-loaded liposomes play a dual antioxidant role in the treatment of osteoarthritis.
35964941	6	46	theme	ChsMA	1214:1218	arg1	Lipo					1220:1223	ChsMA@Lipo	1214:1223	ChsMA@Lipo	1214:1223	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	6	46	theme	ChsMA	1214:1218	arg1	liposomes					1203:1211	liquiritin (LQ)-loaded liposomes	1180:1211	liquiritin (LQ)-loaded liposomes (ChsMA@Lipo)	1180:1224	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	3	47	theme	circulation	450:460	arg1	half-life					462:470	the drug circulation half-life	441:470	the drug circulation half-life	441:470	Currently, the drug circulation half-life can be extended using delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases.
35964941	5	48	theme	antioxidant	955:965	arg1	properties					967:976	good antioxidant properties	950:976	good antioxidant properties	950:976	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	2	49	theme	antioxidant	393:403	arg1	drugs					405:409	antioxidant drugs	393:409	antioxidant drugs	393:409	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	5	50	theme	injectable	1022:1031	arg1	sulfate					1045:1051	an injectable chondroitin sulfate	1019:1051	an injectable chondroitin sulfate hydrogel	1019:1060	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	4	51	theme	new	827:829	arg1	systems					851:857	new local drug delivery systems	827:857	new local drug delivery systems	827:857	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	11	52	theme	ECM	2116:2118	arg1	degradation					2120:2130	IL-1β-induced ECM degradation	2102:2130	IL-1β-induced ECM degradation in chondrocytes	2102:2146	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	5	53	theme	sulfate	1045:1051	arg1	hydrogel					1053:1060	an injectable chondroitin sulfate hydrogel	1019:1060	an injectable chondroitin sulfate hydrogel	1019:1060	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	2	54	from	effect	383:388	arg1	osteoarthritis					414:427	osteoarthritis	414:427	osteoarthritis	414:427	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	6	55	theme	ChsMA	1150:1154	arg1	microgels					1156:1164	ChsMA microgels	1150:1164	ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo)	1150:1224	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	10	56	theme	delivery	2054:2061	arg1	system					2063:2068	a new and efficient drug delivery system	2029:2068	a new and efficient drug delivery system	2029:2068	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	10	57	theme	hydrogel	1865:1872	arg1	microspheres					1874:1885	hydrogel microspheres	1865:1885	hydrogel microspheres	1865:1885	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	5	58	used	used	1003:1006	arg2	sulfate					872:878	Chondroitin sulfate	860:878	Chondroitin sulfate	860:878	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	58	used	used	1003:1006	arg2	glycosaminoglycan					892:908	a sulfated glycosaminoglycan	881:908	a sulfated glycosaminoglycan commonly found in native cartilage	881:943	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	1	59	theme	osteoarthritis	208:221	arg1	pathogenesis					192:203	the pathogenesis	188:203	the pathogenesis of osteoarthritis	188:221	Reactive oxygen species (ROS) play a critical role in the pathogenesis of osteoarthritis.
35964941	7	60	theme	matrix	1497:1502	arg1	network					1504:1510	the hydrogel matrix network	1484:1510	the hydrogel matrix network	1484:1510	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	10	61	theme	efficient	2039:2047	arg1	system					2063:2068	a new and efficient drug delivery system	2029:2068	a new and efficient drug delivery system	2029:2068	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	8	62	theme	sulfate	1593:1599	arg1	monomers					1601:1608	chondroitin sulfate monomers	1581:1608	chondroitin sulfate monomers	1581:1608	On the other hand, ChsMA can eliminate ROS through degradation into chondroitin sulfate monomers by enzymes in vivo.
35964941	2	63	theme	articular	340:348	arg1	cavity					350:355	the articular cavity	336:355	the articular cavity	336:355	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	7	64	theme	membrane	1471:1478	arg1	obstruction					1446:1456	the double obstruction	1435:1456	the double obstruction of the lipid membrane and the hydrogel matrix network	1435:1510	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	9	65	theme	ChsMA	1641:1645	arg1	option					1729:1734	a promising option	1717:1734	a promising option for osteoarthritis treatment	1717:1763	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	9	65	theme	ChsMA	1641:1645	arg1	Lipo					1647:1650	ChsMA@Lipo	1641:1650	ChsMA@Lipo	1641:1650	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	7	66	theme	double	1439:1444	arg1	obstruction					1446:1456	the double obstruction	1435:1456	the double obstruction of the lipid membrane and the hydrogel matrix network	1435:1510	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	4	67	theme	local	831:835	arg1	systems					851:857	new local drug delivery systems	827:857	new local drug delivery systems	827:857	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	1	68	theme	critical	171:178	arg1	role					180:183	a critical role	169:183	a critical role	169:183	Reactive oxygen species (ROS) play a critical role in the pathogenesis of osteoarthritis.
35964941	4	69	theme	delivery	842:849	arg1	systems					851:857	new local drug delivery systems	827:857	new local drug delivery systems	827:857	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	4	70	theme	microspheres	651:662	arg1	carriers					616:623	the composite carriers	602:623	the composite carriers of liposomes and hydrogel microspheres	602:662	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	2	71	theme	drug	291:294	arg1	utilization					296:306	low drug utilization	287:306	low drug utilization	287:306	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	9	72	theme	dual	1673:1676	arg1	platform					1704:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	7	73	theme	significant	1389:1399	arg1	kinetics					1419:1426	significant sustained-release kinetics	1389:1426	significant sustained-release kinetics	1389:1426	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	5	74	contain	has	946:948	arg1	glycosaminoglycan					892:908	a sulfated glycosaminoglycan	881:908	a sulfated glycosaminoglycan commonly found in native cartilage	881:943	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	74	contain	has	946:948	arg2	degradability					982:994	degradability	982:994	degradability	982:994	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	74	contain	has	946:948	arg1	sulfate					872:878	Chondroitin sulfate	860:878	Chondroitin sulfate	860:878	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	74	contain	has	946:948	arg2	properties					967:976	good antioxidant properties	950:976	good antioxidant properties	950:976	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	4	75	theme	composite	606:614	arg1	carriers					616:623	the composite carriers	602:623	the composite carriers of liposomes and hydrogel microspheres	602:662	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	10	76	theme	materials	1943:1951	arg1	advantages					1919:1928	the advantages	1915:1928	the advantages of different materials, which shows stronger plasticity and flexibility	1915:2000	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	9	77	theme	degradable	1658:1667	arg1	platform					1704:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	9	78	theme	drug	1690:1693	arg1	platform					1704:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	a degradable and dual antioxidant drug delivery platform	1656:1711	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	10	79	theme	SIGNIFICANCE	1779:1790	arg1	STATEMENT					1766:1774	STATEMENT	1766:1774	STATEMENT OF SIGNIFICANCE	1766:1790	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	12	80	theme	osteoarthritis	2285:2298	arg1	progression					2270:2280	the progression	2266:2280	the progression of osteoarthritis	2266:2298	The obtained ChsMA@Lipo alleviates the progression of osteoarthritis in vivo, which is promising for osteoarthritis treatment.
35964941	11	81	theme	@	2076:2076	arg1	Lipo					2077:2080	ChsMA@Lipo	2071:2080	ChsMA@Lipo	2071:2080	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	7	82	theme	@	1364:1364	arg1	microgels					1370:1378	ChsMA@Lipo microgels	1359:1378	ChsMA@Lipo microgels	1359:1378	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35964941	4	83	theme	different	694:702	arg1	forms					713:717	different material forms	694:717	different material forms	694:717	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	10	84	theme	stronger	1966:1973	arg1	plasticity					1975:1984	stronger plasticity	1966:1984	stronger plasticity	1966:1984	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	2	85	theme	antioxidant	250:260	arg1	drug					262:265	a single antioxidant drug	241:265	a single antioxidant drug	241:265	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	5	86	theme	native	928:933	arg1	cartilage					935:943	native cartilage	928:943	native cartilage	928:943	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	6	87	theme	@	1219:1219	arg1	Lipo					1220:1223	ChsMA@Lipo	1214:1223	ChsMA@Lipo	1214:1223	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	6	87	theme	@	1219:1219	arg1	liposomes					1203:1211	liquiritin (LQ)-loaded liposomes	1180:1211	liquiritin (LQ)-loaded liposomes (ChsMA@Lipo)	1180:1224	Herein, ChsMA microgels anchored with liquiritin (LQ)-loaded liposomes (ChsMA@Lipo) were developed to delay the progression of osteoarthritis by dual antioxidation.
35964941	4	88	theme	forms	713:717	arg1	advantages					680:689	the advantages	676:689	the advantages of different material forms	676:717	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	2	89	theme	residence	318:326	arg1	time					328:331	short residence time	312:331	short residence time	312:331	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	0	90	theme	dual	76:79	arg1	role					93:96	a dual antioxidant role	74:96	a dual antioxidant role	74:96	Chondroitin sulfate microspheres anchored with drug-loaded liposomes play a dual antioxidant role in the treatment of osteoarthritis.
35964941	9	91	theme	promising	1719:1727	arg1	option					1729:1734	a promising option	1717:1734	a promising option for osteoarthritis treatment	1717:1763	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	9	91	theme	promising	1719:1727	arg1	Lipo					1647:1650	ChsMA@Lipo	1641:1650	ChsMA@Lipo	1641:1650	Therefore, ChsMA@Lipo, as a degradable and dual antioxidant drug delivery platform, is a promising option for osteoarthritis treatment.
35964941	5	92	located	found	919:923	arg2	glycosaminoglycan					892:908	a sulfated glycosaminoglycan	881:908	a sulfated glycosaminoglycan commonly found in native cartilage	881:943	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	92	located	found	919:923	arg2	sulfate					872:878	Chondroitin sulfate	860:878	Chondroitin sulfate	860:878	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	92	located	found	919:923	arg1	cartilage					935:943	native cartilage	928:943	native cartilage	928:943	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	8	93	theme	other	1520:1524	arg1	hand					1526:1529	the other hand	1516:1529	the other hand	1516:1529	On the other hand, ChsMA can eliminate ROS through degradation into chondroitin sulfate monomers by enzymes in vivo.
35964941	4	94	theme	traditional	769:779	arg1	carriers					788:795	traditional single carriers	769:795	traditional single carriers	769:795	In addition, the composite carriers of liposomes and hydrogel microspheres can combine the advantages of different material forms and show stronger plasticity and flexibility than traditional single carriers, which are expected to become new local drug delivery systems.
35964941	2	95	theme	therapeutic	371:381	arg1	effect					383:388	the therapeutic effect	367:388	the therapeutic effect of antioxidant drugs on osteoarthritis	367:427	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	0	96	theme	sulfate	12:18	arg1	microspheres					20:31	Chondroitin sulfate microspheres	0:31	Chondroitin sulfate microspheres anchored with drug-loaded liposomes	0:67	Chondroitin sulfate microspheres anchored with drug-loaded liposomes play a dual antioxidant role in the treatment of osteoarthritis.
35964941	5	97	theme	photo-cross-linkable	1092:1111	arg1	ChsMA					1134:1138	ChsMA	1134:1138	ChsMA	1134:1138	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	5	97	theme	photo-cross-linkable	1092:1111	arg1	groups					1126:1131	photo-cross-linkable methacryloyl groups	1092:1131	photo-cross-linkable methacryloyl groups (ChsMA)	1092:1139	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	12	98	dep	alleviates	2255:2264	arg1	promising					2318:2326	promising	2318:2326	promising	2318:2326	The obtained ChsMA@Lipo alleviates the progression of osteoarthritis in vivo, which is promising for osteoarthritis treatment.
35964941	5	99	theme	good	950:953	arg1	properties					967:976	good antioxidant properties	950:976	good antioxidant properties	950:976	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	12	100	theme	ChsMA	2244:2248	arg1	Lipo					2250:2253	The obtained ChsMA@Lipo	2231:2253	The obtained ChsMA@Lipo	2231:2253	The obtained ChsMA@Lipo alleviates the progression of osteoarthritis in vivo, which is promising for osteoarthritis treatment.
35964941	10	101	theme	composite	1843:1851	arg1	carriers					1853:1860	the composite carriers	1839:1860	the composite carriers of hydrogel microspheres and liposome	1839:1898	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	2	102	theme	drugs	405:409	arg1	effect					383:388	the therapeutic effect	367:388	the therapeutic effect of antioxidant drugs on osteoarthritis	367:427	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	0	103	theme	drug-loaded	47:57	arg1	liposomes					59:67	drug-loaded liposomes	47:67	drug-loaded liposomes	47:67	Chondroitin sulfate microspheres anchored with drug-loaded liposomes play a dual antioxidant role in the treatment of osteoarthritis.
35964941	3	104	used	used	565:568	arg2	liposomes					520:528	liposomes	520:528	liposomes	520:528	Currently, the drug circulation half-life can be extended using delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases.
35964941	3	104	used	used	565:568	arg2	microspheres					534:545	microspheres	534:545	microspheres	534:545	Currently, the drug circulation half-life can be extended using delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases.
35964941	3	105	theme	drug	445:448	arg1	half-life					462:470	the drug circulation half-life	441:470	the drug circulation half-life	441:470	Currently, the drug circulation half-life can be extended using delivery vehicles such as liposomes and microspheres, which are widely used to treat diseases.
35964941	5	106	theme	chondroitin	1033:1043	arg1	sulfate					1045:1051	an injectable chondroitin sulfate	1019:1051	an injectable chondroitin sulfate hydrogel	1019:1060	Chondroitin sulfate, a sulfated glycosaminoglycan commonly found in native cartilage, has good antioxidant properties and degradability and is used to develop an injectable chondroitin sulfate hydrogel by covalent modification with photo-cross-linkable methacryloyl groups (ChsMA).
35964941	11	107	theme	M1	2170:2171	arg1	activation					2219:2228	the inflammasome activation	2202:2228	the inflammasome activation	2202:2228	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	11	107	theme	M1	2170:2171	arg1	macrophages					2173:2183	the M1 macrophages polarization and the inflammasome activation	2166:2228	the M1 macrophages polarization and the inflammasome activation	2166:2228	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	11	107	theme	M1	2170:2171	arg1	polarization					2185:2196	polarization	2185:2196	polarization	2185:2196	ChsMA@Lipo not only attenuates IL-1β-induced ECM degradation in chondrocytes but also inhibits the M1 macrophages polarization and the inflammasome activation.
35964941	8	108	theme	chondroitin	1581:1591	arg1	sulfate					1593:1599	chondroitin sulfate	1581:1599	chondroitin sulfate monomers	1581:1608	On the other hand, ChsMA can eliminate ROS through degradation into chondroitin sulfate monomers by enzymes in vivo.
35964941	10	109	theme	microspheres	1874:1885	arg1	carriers					1853:1860	the composite carriers	1839:1860	the composite carriers of hydrogel microspheres and liposome	1839:1898	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	10	110	theme	drug	2049:2052	arg1	system					2063:2068	a new and efficient drug delivery system	2029:2068	a new and efficient drug delivery system	2029:2068	STATEMENT OF SIGNIFICANCE: Compared with the traditional single carrier, the composite carriers of hydrogel microspheres and liposome can complement the advantages of different materials, which shows stronger plasticity and flexibility, and is expected to become a new and efficient drug delivery system.
35964941	12	111	theme	obtained	2235:2242	arg1	Lipo					2250:2253	The obtained ChsMA@Lipo	2231:2253	The obtained ChsMA@Lipo	2231:2253	The obtained ChsMA@Lipo alleviates the progression of osteoarthritis in vivo, which is promising for osteoarthritis treatment.
35964941	2	112	theme	short	312:316	arg1	time					328:331	short residence time	312:331	short residence time	312:331	The injection of a single antioxidant drug is characterized by low drug utilization and short residence time in the articular cavity, limiting the therapeutic effect of antioxidant drugs on osteoarthritis.
35964941	7	113	theme	network	1504:1510	arg1	obstruction					1446:1456	the double obstruction	1435:1456	the double obstruction of the lipid membrane and the hydrogel matrix network	1435:1510	On the one hand, the antioxidant drug LQ wrapped in ChsMA@Lipo microgels exhibits significant sustained-release kinetics due to the double obstruction of the lipid membrane and the hydrogel matrix network.
35816908	4	0	theme	X-ray	738:742	arg1	spectroscopy					758:769	X-ray photoelectron spectroscopy	738:769	X-ray photoelectron spectroscopy	738:769	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	1	1	theme	amperometric	170:181	arg1	determination					183:195	amperometric determination	170:195	amperometric determination of alpha-fetoprotein (AFP)	170:222	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	9	2	from	detection	1448:1456	arg1	serum					1474:1478	human serum	1468:1478	human serum	1468:1478	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	4	3	dep	Fourier	697:703	arg1	transform					705:713	transform	705:713	transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy	705:886	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	6	4	used	used	1073:1076	arg2	voltammetry					1056:1066	cyclic voltammetry	1049:1066	cyclic voltammetry	1049:1066	Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the assembly process of immunosensor.
35816908	6	4	used	used	1073:1076	arg2	spectroscopy					1032:1043	Electrochemical impedance spectroscopy	1006:1043	Electrochemical impedance spectroscopy	1006:1043	Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the assembly process of immunosensor.
35816908	0	5	theme	AFP	116:118	arg1	detection					103:111	the detection	99:111	the detection of AFP	99:118	An ultrasensitive immunosensor based on cellulose nanofibrils/polydopamine/Cu-Ag nanocomposite for the detection of AFP.
35816908	1	6	theme	signal	368:373	arg1	amplifier					375:383	signal amplifier	368:383	signal amplifier	368:383	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	7	7	theme	Cu-Ag	1208:1212	arg1	nanoparticles					1214:1226	Cu-Ag nanoparticles	1208:1226	Cu-Ag nanoparticles	1208:1226	The immunoreaction was amplified by electrocatalytical reduction of H2O2 on Cu-Ag nanoparticles, through which AFP was quantitatively detected.
35816908	4	8	theme	electron	807:814	arg1	microscopy					816:825	scanning electron microscopy	798:825	scanning electron microscopy	798:825	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	8	9	theme	detection	1367:1375	arg1	limit					1377:1381	low detection limit	1363:1381	low detection limit of 4.27 pg mL-1 (S/N = 3)	1363:1407	The developed sensor exhibits wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963) with low detection limit of 4.27 pg mL-1 (S/N = 3).
35816908	9	10	theme	human	1468:1472	arg1	serum					1474:1478	human serum	1468:1478	human serum	1468:1478	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	5	11	theme	modified	908:915	arg1	electrode					931:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode can fix AFP antibody (Ab), and further capture AFP specifically.
35816908	4	12	theme	synthesized	634:644	arg1	nanocomposite					661:673	the synthesized CNFs/PDA/Cu-Ag nanocomposite	630:673	the synthesized CNFs/PDA/Cu-Ag nanocomposite	630:673	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	4	13	theme	particle	828:835	arg1	analyzer					842:849	particle size analyzer	828:849	particle size analyzer	828:849	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	9	14	theme	application	1508:1518	arg1	prospect					1520:1527	its preeminent application prospect	1493:1527	its preeminent application prospect	1493:1527	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	9	15	theme	liver	1538:1542	arg1	diagnosis					1551:1559	early liver cancer diagnosis	1532:1559	early liver cancer diagnosis	1532:1559	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	7	16	theme	H2O2	1200:1203	arg1	reduction					1187:1195	electrocatalytical reduction	1168:1195	electrocatalytical reduction of H2O2 on Cu-Ag nanoparticles, through which AFP was quantitatively detected	1168:1273	The immunoreaction was amplified by electrocatalytical reduction of H2O2 on Cu-Ag nanoparticles, through which AFP was quantitatively detected.
35816908	3	17	theme	Cu-Ag	546:550	arg1	nanoparticles					552:564	Cu-Ag nanoparticles	546:564	Cu-Ag nanoparticles	546:564	The obtained CNFs/PDA was applied as substrate to electrolessly deposit Cu-Ag nanoparticles, using NaBH4 as reducing agent.
35816908	1	18	theme	alpha-fetoprotein	200:216	arg1	determination					183:195	amperometric determination	170:195	amperometric determination of alpha-fetoprotein (AFP)	170:222	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	0	19	theme	ultrasensitive	3:16	arg1	immunosensor					18:29	An ultrasensitive immunosensor	0:29	An ultrasensitive immunosensor based on cellulose nanofibrils/polydopamine/Cu-Ag	0:79	An ultrasensitive immunosensor based on cellulose nanofibrils/polydopamine/Cu-Ag nanocomposite for the detection of AFP.
35816908	6	20	theme	impedance	1022:1030	arg1	spectroscopy					1032:1043	Electrochemical impedance spectroscopy	1006:1043	Electrochemical impedance spectroscopy	1006:1043	Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the assembly process of immunosensor.
35816908	7	21	theme	electrocatalytical	1168:1185	arg1	reduction					1187:1195	electrocatalytical reduction	1168:1195	electrocatalytical reduction of H2O2 on Cu-Ag nanoparticles, through which AFP was quantitatively detected	1168:1273	The immunoreaction was amplified by electrocatalytical reduction of H2O2 on Cu-Ag nanoparticles, through which AFP was quantitatively detected.
35816908	9	22	from	serum	1474:1478	arg1	detection					1448:1456	the detection	1444:1456	the detection of AFP in human serum	1444:1478	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	9	23	theme	cancer	1544:1549	arg1	diagnosis					1551:1559	early liver cancer diagnosis	1532:1559	early liver cancer diagnosis	1532:1559	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	8	24	theme	developed	1280:1288	arg1	sensor					1290:1295	The developed sensor	1276:1295	The developed sensor	1276:1295	The developed sensor exhibits wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963) with low detection limit of 4.27 pg mL-1 (S/N = 3).
35816908	6	25	theme	cyclic	1049:1054	arg1	voltammetry					1056:1066	cyclic voltammetry	1049:1066	cyclic voltammetry	1049:1066	Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the assembly process of immunosensor.
35816908	3	26	theme	reducing	582:589	arg1	agent					591:595	reducing agent	582:595	reducing agent	582:595	The obtained CNFs/PDA was applied as substrate to electrolessly deposit Cu-Ag nanoparticles, using NaBH4 as reducing agent.
35816908	8	27	theme	low	1363:1365	arg1	limit					1377:1381	low detection limit	1363:1381	low detection limit of 4.27 pg mL-1 (S/N = 3)	1363:1407	The developed sensor exhibits wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963) with low detection limit of 4.27 pg mL-1 (S/N = 3).
35816908	5	28	theme	AFP	949:951	arg1	Ab					963:964	Ab	963:964	Ab	963:964	The CNFs/PDA/Cu-Ag modified glassy carbon electrode can fix AFP antibody (Ab), and further capture AFP specifically.
35816908	5	28	theme	AFP	949:951	arg1	antibody					953:960	AFP antibody	949:960	AFP antibody (Ab)	949:965	The CNFs/PDA/Cu-Ag modified glassy carbon electrode can fix AFP antibody (Ab), and further capture AFP specifically.
35816908	8	29	theme	wide	1306:1309	arg1	range					1318:1322	wide linear range	1306:1322	wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963)	1306:1356	The developed sensor exhibits wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963) with low detection limit of 4.27 pg mL-1 (S/N = 3).
35816908	1	30	theme	functionalized	294:307	arg1	CNFs/PDA/Cu-Ag					349:362	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	4	31	theme	scanning	798:805	arg1	microscopy					816:825	scanning electron microscopy	798:825	scanning electron microscopy	798:825	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	4	32	theme	CNFs/PDA/Cu-Ag	646:659	arg1	nanocomposite					661:673	the synthesized CNFs/PDA/Cu-Ag nanocomposite	630:673	the synthesized CNFs/PDA/Cu-Ag nanocomposite	630:673	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	4	33	theme	powder	778:783	arg1	diffraction					785:795	X-ray powder diffraction	772:795	X-ray powder diffraction	772:795	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	1	34	theme	cellulose	309:317	arg1	CNFs/PDA/Cu-Ag					349:362	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	4	35	dep	structure	602:610	arg1	The					598:600	The	598:600	The	598:600	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	9	36	theme	preeminent	1497:1506	arg1	prospect					1520:1527	its preeminent application prospect	1493:1527	its preeminent application prospect	1493:1527	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	2	37	theme	dopamine	433:440	arg1	self-polymerizing					412:428	self-polymerizing	412:428	self-polymerizing of dopamine	412:440	PDA was first prepared by self-polymerizing of dopamine, and then was adsorbed on CNFs.
35816908	0	38	theme	cellulose	40:48	arg1	nanofibrils/polydopamine/Cu-Ag					50:79	cellulose nanofibrils/polydopamine/Cu-Ag	40:79	cellulose nanofibrils/polydopamine/Cu-Ag	40:79	An ultrasensitive immunosensor based on cellulose nanofibrils/polydopamine/Cu-Ag nanocomposite for the detection of AFP.
35816908	1	39	theme	nanofibrils	319:329	arg1	CNFs/PDA/Cu-Ag					349:362	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	6	40	theme	immunosensor	1118:1129	arg1	process					1107:1113	the assembly process	1094:1113	the assembly process of immunosensor	1094:1129	Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the assembly process of immunosensor.
35816908	9	41	theme	early	1532:1536	arg1	diagnosis					1551:1559	early liver cancer diagnosis	1532:1559	early liver cancer diagnosis	1532:1559	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	5	42	theme	glassy	917:922	arg1	electrode					931:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode can fix AFP antibody (Ab), and further capture AFP specifically.
35816908	8	43	theme	linear	1311:1316	arg1	range					1318:1322	wide linear range	1306:1322	wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963)	1306:1356	The developed sensor exhibits wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963) with low detection limit of 4.27 pg mL-1 (S/N = 3).
35816908	6	44	theme	Electrochemical	1006:1020	arg1	spectroscopy					1032:1043	Electrochemical impedance spectroscopy	1006:1043	Electrochemical impedance spectroscopy	1006:1043	Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the assembly process of immunosensor.
35816908	4	45	theme	electron	868:875	arg1	microscopy					877:886	transmission electron microscopy	855:886	transmission electron microscopy	855:886	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	4	46	theme	nanocomposite	661:673	arg1	morphology					616:625	morphology	616:625	morphology	616:625	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	4	46	theme	nanocomposite	661:673	arg1	structure					602:610	structure	602:610	structure	602:610	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	8	47	theme	0.01-100 ng mL-1	1327:1342	arg1	range					1318:1322	wide linear range	1306:1322	wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963)	1306:1356	The developed sensor exhibits wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963) with low detection limit of 4.27 pg mL-1 (S/N = 3).
35816908	1	48	theme	CNFs	332:335	arg1	CNFs/PDA/Cu-Ag					349:362	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	7	49	from	reduction	1187:1195	arg1	nanoparticles					1214:1226	Cu-Ag nanoparticles	1208:1226	Cu-Ag nanoparticles	1208:1226	The immunoreaction was amplified by electrocatalytical reduction of H2O2 on Cu-Ag nanoparticles, through which AFP was quantitatively detected.
35816908	9	50	from	AFP	1461:1463	arg1	serum					1474:1478	human serum	1468:1478	human serum	1468:1478	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	9	51	used	used	1435:1438	arg2	it					1423:1424	it	1423:1424	it	1423:1424	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	1	52	from	nanoparticles	258:270	arg1	CNFs/PDA/Cu-Ag					349:362	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	4	53	theme	photoelectron	744:756	arg1	spectroscopy					758:769	X-ray photoelectron spectroscopy	738:769	X-ray photoelectron spectroscopy	738:769	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	1	54	theme	composite	338:346	arg1	CNFs/PDA/Cu-Ag					349:362	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	1	55	from	Ag	248:249	arg1	CNFs/PDA/Cu-Ag					349:362	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag)	275:363	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	6	56	theme	assembly	1098:1105	arg1	process					1107:1113	the assembly process	1094:1113	the assembly process of immunosensor	1094:1129	Electrochemical impedance spectroscopy and cyclic voltammetry were used to characterize the assembly process of immunosensor.
35816908	4	57	theme	X-ray	772:776	arg1	diffraction					785:795	X-ray powder diffraction	772:795	X-ray powder diffraction	772:795	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	9	58	theme	AFP	1461:1463	arg1	detection					1448:1456	the detection	1444:1456	the detection of AFP in human serum	1444:1478	In addition, it has been used for the detection of AFP in human serum, manifesting its preeminent application prospect in early liver cancer diagnosis.
35816908	4	59	theme	size	837:840	arg1	analyzer					842:849	particle size analyzer	828:849	particle size analyzer	828:849	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	1	60	theme	ultrasensitive	138:151	arg1	immunosensor					153:164	an ultrasensitive immunosensor	135:164	an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP)	135:222	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	3	61	theme	obtained	478:485	arg1	substrate					511:519	substrate	511:519	substrate	511:519	The obtained CNFs/PDA was applied as substrate to electrolessly deposit Cu-Ag nanoparticles, using NaBH4 as reducing agent.
35816908	3	61	theme	obtained	478:485	arg1	CNFs/PDA					487:494	The obtained CNFs/PDA	474:494	The obtained CNFs/PDA	474:494	The obtained CNFs/PDA was applied as substrate to electrolessly deposit Cu-Ag nanoparticles, using NaBH4 as reducing agent.
35816908	5	62	theme	carbon	924:929	arg1	electrode					931:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode can fix AFP antibody (Ab), and further capture AFP specifically.
35816908	4	63	theme	transmission	855:866	arg1	microscopy					877:886	transmission electron microscopy	855:886	transmission electron microscopy	855:886	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	5	64	theme	CNFs/PDA/Cu-Ag	893:906	arg1	electrode					931:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode	889:939	The CNFs/PDA/Cu-Ag modified glassy carbon electrode can fix AFP antibody (Ab), and further capture AFP specifically.
35816908	1	65	theme	Cu	255:256	arg1	nanoparticles					258:270	Cu nanoparticles	255:270	Cu nanoparticles	255:270	In this work, an ultrasensitive immunosensor for amperometric determination of alpha-fetoprotein (AFP) was developed utilizing Ag and Cu nanoparticles on polydopamine (PDA) functionalized cellulose nanofibrils (CNFs) composite (CNFs/PDA/Cu-Ag) as signal amplifier.
35816908	4	66	theme	infrared	715:722	arg1	spectroscopy					724:735	infrared spectroscopy	715:735	infrared spectroscopy	715:735	The structure and morphology of the synthesized CNFs/PDA/Cu-Ag nanocomposite were analyzed through Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, scanning electron microscopy, particle size analyzer and transmission electron microscopy.
35816908	8	67	theme	4.27 pg mL-1	1386:1397	arg1	limit					1377:1381	low detection limit	1363:1381	low detection limit of 4.27 pg mL-1 (S/N = 3)	1363:1407	The developed sensor exhibits wide linear range of 0.01-100 ng mL-1 (R2 = 0.9963) with low detection limit of 4.27 pg mL-1 (S/N = 3).
37224635	0	0	theme	chilled	99:105	arg1	preservation					115:126	chilled chicken preservation	99:126	chilled chicken preservation	99:126	Novel PVA/carboxylated cellulose antimicrobial hydrogel grafted with curcumin and ε-polylysine for chilled chicken preservation.
37224635	1	1	contain	containing	159:168	arg2	ε-polylysine					189:200	ε-polylysine	189:200	ε-polylysine (PL)	189:205	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	1	1	contain	containing	159:168	arg2	CUR					180:182	CUR	180:182	CUR	180:182	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	1	1	contain	containing	159:168	arg2	curcumin					170:177	curcumin	170:177	curcumin (CUR)	170:183	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	1	1	contain	containing	159:168	arg1	films					153:157	PVA/CC/CUR/PL composite films	129:157	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL)	129:205	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	1	1	contain	containing	159:168	arg2	PL					203:204	PL	203:204	PL	203:204	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	7	2	theme	good	1135:1138	arg1	properties					1151:1160	good mechanical properties	1135:1160	good mechanical properties	1135:1160	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	2	3	theme	PL	361:362	arg1	grafting					341:348	the grafting	337:348	the grafting of CUR and PL	337:362	Morphological analysis showed that the grafting of CUR and PL resulted in a rough cross-section of the polymer matrix.
37224635	7	4	theme	mechanical	1140:1149	arg1	properties					1151:1160	good mechanical properties	1135:1160	good mechanical properties	1135:1160	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	5	5	theme	PVA/CC/CUR/PL	729:741	arg1	films					743:747	The PVA/CC/CUR/PL films	725:747	The PVA/CC/CUR/PL films	725:747	The PVA/CC/CUR/PL films exhibited strong bactericidal activity, reaching 99.0% and 99.8% for Pseudomonas lundensis and Shewanella putrefaciens, respectively.
37224635	6	6	theme	total	912:916	arg1	number					918:923	the total number	908:923	the total number of colonies	908:935	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	3	7	theme	CUR	531:533	arg1	grafting					519:526	the grafting	515:526	the grafting of CUR and PL into the polymer matrix	515:564	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	2	8	theme	Morphological	302:314	arg1	analysis					316:323	Morphological analysis	302:323	Morphological analysis	302:323	Morphological analysis showed that the grafting of CUR and PL resulted in a rough cross-section of the polymer matrix.
37224635	0	9	theme	chicken	107:113	arg1	preservation					115:126	chilled chicken preservation	99:126	chilled chicken preservation	99:126	Novel PVA/carboxylated cellulose antimicrobial hydrogel grafted with curcumin and ε-polylysine for chilled chicken preservation.
37224635	2	10	theme	CUR	353:355	arg1	grafting					341:348	the grafting	337:348	the grafting of CUR and PL	337:362	Morphological analysis showed that the grafting of CUR and PL resulted in a rough cross-section of the polymer matrix.
37224635	7	11	theme	promising	1098:1106	arg1	films					1072:1076	PVA/CC/CUR/PL films	1058:1076	PVA/CC/CUR/PL films	1058:1076	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	7	11	theme	promising	1098:1106	arg1	material					1121:1128	a promising bactericidal material	1096:1128	a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics	1096:1212	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	3	12	theme	infrared	439:446	arg1	FTIR					462:465	FTIR	462:465	FTIR	462:465	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	3	12	theme	infrared	439:446	arg1	spectroscopy					448:459	infrared spectroscopy	439:459	infrared spectroscopy (FTIR)	439:466	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	5	13	theme	strong	759:764	arg1	activity					779:786	strong bactericidal activity	759:786	strong bactericidal activity	759:786	The PVA/CC/CUR/PL films exhibited strong bactericidal activity, reaching 99.0% and 99.8% for Pseudomonas lundensis and Shewanella putrefaciens, respectively.
37224635	6	14	theme	storage	899:905	arg1	8 days					889:894	8 days	889:894	8 days of storage	889:905	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	7	15	theme	bactericidal	1108:1119	arg1	films					1072:1076	PVA/CC/CUR/PL films	1058:1076	PVA/CC/CUR/PL films	1058:1076	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	7	15	theme	bactericidal	1108:1119	arg1	material					1121:1128	a promising bactericidal material	1096:1128	a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics	1096:1212	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	0	16	theme	PVA/carboxylated	6:21	arg1	hydrogel					47:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel grafted with curcumin and ε-polylysine for chilled chicken preservation.
37224635	6	17	from	number	918:923	arg1	group					980:984	the PVA/CC/CUR/PL group	962:984	the PVA/CC/CUR/PL group	962:984	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	2	18	theme	rough	378:382	arg1	cross-section					384:396	a rough cross-section	376:396	a rough cross-section of the polymer matrix	376:418	Morphological analysis showed that the grafting of CUR and PL resulted in a rough cross-section of the polymer matrix.
37224635	0	19	theme	Novel	0:4	arg1	hydrogel					47:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel grafted with curcumin and ε-polylysine for chilled chicken preservation.
37224635	3	20	theme	polymer	551:557	arg1	matrix					559:564	the polymer matrix	547:564	the polymer matrix	547:564	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	0	21	theme	antimicrobial	33:45	arg1	hydrogel					47:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel grafted with curcumin and ε-polylysine for chilled chicken preservation.
37224635	1	22	theme	food	287:290	arg1	spoilage					292:299	food spoilage	287:299	food spoilage	287:299	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	3	23	theme	PL	539:540	arg1	grafting					519:526	the grafting	515:526	the grafting of CUR and PL into the polymer matrix	515:564	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	3	24	dep	Fourier	421:427	arg1	transform					429:437	transform	429:437	transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis	429:503	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	4	25	theme	Thermal	624:630	arg1	loss					639:642	Thermal weight loss	624:642	Thermal weight loss analysis	624:651	Thermal weight loss analysis showed that grafting process positively improved the thermal stability.
37224635	0	26	theme	cellulose	23:31	arg1	hydrogel					47:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel	0:54	Novel PVA/carboxylated cellulose antimicrobial hydrogel grafted with curcumin and ε-polylysine for chilled chicken preservation.
37224635	1	27	theme	PVA/CC/CUR/PL	129:141	arg1	films					153:157	PVA/CC/CUR/PL composite films	129:157	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL)	129:205	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	2	28	theme	matrix	413:418	arg1	cross-section					384:396	a rough cross-section	376:396	a rough cross-section of the polymer matrix	376:418	Morphological analysis showed that the grafting of CUR and PL resulted in a rough cross-section of the polymer matrix.
37224635	7	29	theme	PVA/CC/CUR/PL	1058:1070	arg1	films					1072:1076	PVA/CC/CUR/PL films	1058:1076	PVA/CC/CUR/PL films	1058:1076	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	7	29	theme	PVA/CC/CUR/PL	1058:1070	arg1	material					1121:1128	a promising bactericidal material	1096:1128	a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics	1096:1212	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	4	30	theme	loss	639:642	arg1	analysis					644:651	Thermal weight loss analysis	624:651	Thermal weight loss analysis	624:651	Thermal weight loss analysis showed that grafting process positively improved the thermal stability.
37224635	3	31	theme	esterification	570:583	arg1	reactions					599:607	esterification and amidation reactions	570:607	esterification and amidation reactions	570:607	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	2	32	theme	polymer	405:411	arg1	matrix					413:418	the polymer matrix	401:418	the polymer matrix	401:418	Morphological analysis showed that the grafting of CUR and PL resulted in a rough cross-section of the polymer matrix.
37224635	4	33	theme	weight	632:637	arg1	loss					639:642	Thermal weight loss	624:642	Thermal weight loss analysis	624:651	Thermal weight loss analysis showed that grafting process positively improved the thermal stability.
37224635	6	34	theme	TVB-N	945:949	arg1	content					951:957	the TVB-N content	941:957	the TVB-N content in the PVA/CC/CUR/PL group	941:984	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	6	35	theme	PVA/CC/CUR/PL	966:978	arg1	group					980:984	the PVA/CC/CUR/PL group	962:984	the PVA/CC/CUR/PL group	962:984	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	1	36	theme	composite	143:151	arg1	films					153:157	PVA/CC/CUR/PL composite films	129:157	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL)	129:205	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	4	37	theme	thermal	706:712	arg1	stability					714:722	the thermal stability	702:722	the thermal stability	702:722	Thermal weight loss analysis showed that grafting process positively improved the thermal stability.
37224635	6	38	from	content	951:957	arg1	group					980:984	the PVA/CC/CUR/PL group	962:984	the PVA/CC/CUR/PL group	962:984	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	3	39	theme	X-ray	472:476	arg1	XRD					491:493	XRD	491:493	XRD	491:493	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	3	39	theme	X-ray	472:476	arg1	diffraction					478:488	X-ray diffraction	472:488	X-ray diffraction (XRD) analysis	472:503	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	3	40	theme	diffraction	478:488	arg1	analysis					496:503	X-ray diffraction (XRD) analysis	472:503	X-ray diffraction (XRD) analysis	472:503	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	1	41	theme	chemical	236:243	arg1	methods					254:260	chemical grafting methods	236:260	chemical grafting methods	236:260	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	5	42	theme	bactericidal	766:777	arg1	activity					779:786	strong bactericidal activity	759:786	strong bactericidal activity	759:786	The PVA/CC/CUR/PL films exhibited strong bactericidal activity, reaching 99.0% and 99.8% for Pseudomonas lundensis and Shewanella putrefaciens, respectively.
37224635	3	43	theme	amidation	589:597	arg1	reactions					599:607	esterification and amidation reactions	570:607	esterification and amidation reactions	570:607	Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) analysis confirmed the grafting of CUR and PL into the polymer matrix via esterification and amidation reactions, respectively.
37224635	7	44	with	material	1121:1128	arg1	functionality					1163:1175	functionality	1163:1175	functionality	1163:1175	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	7	44	with	material	1121:1128	arg1	properties					1151:1160	good mechanical properties	1135:1160	good mechanical properties	1135:1160	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	7	44	with	material	1121:1128	arg1	characteristics					1198:1212	other excellent characteristics	1182:1212	other excellent characteristics	1182:1212	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	1	45	theme	grafting	245:252	arg1	methods					254:260	chemical grafting methods	236:260	chemical grafting methods	236:260	PVA/CC/CUR/PL composite films containing curcumin (CUR) and ε-polylysine (PL) were prepared by casting and chemical grafting methods to address the threat to food spoilage.
37224635	6	46	theme	colonies	928:935	arg1	number					918:923	the total number	908:923	the total number of colonies	908:935	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	6	46	theme	colonies	928:935	arg1	content					951:957	the TVB-N content	941:957	the TVB-N content in the PVA/CC/CUR/PL group	941:984	After 8 days of storage, the total number of colonies and the TVB-N content in the PVA/CC/CUR/PL group decreased by 1.51 lg CFU/g and 13.77 mg/100 g, respectively.
37224635	7	47	theme	other	1182:1186	arg1	characteristics					1198:1212	other excellent characteristics	1182:1212	other excellent characteristics	1182:1212	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
37224635	7	48	theme	excellent	1188:1196	arg1	characteristics					1198:1212	other excellent characteristics	1182:1212	other excellent characteristics	1182:1212	Therefore, PVA/CC/CUR/PL films are considered as a promising bactericidal material with good mechanical properties, functionality, and other excellent characteristics.
35427747	4	0	theme	5-FU	952:955	arg1	loading					941:947	physical loading	932:947	physical loading of 5-FU	932:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	0	theme	5-FU	952:955	arg1	oligomers					898:906	chitosan oligomers	889:906	chitosan oligomers (EDC/NHS chemistry)	889:926	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	0	theme	5-FU	952:955	arg1	chemistry					917:925	EDC/NHS chemistry	909:925	EDC/NHS chemistry	909:925	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	3	1	theme	oligomeric	548:557	arg1	nanoparticles					582:594	oligomeric chitosan coated silver nanoparticles	548:594	oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer	548:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	6	2	theme	oxidant/antioxidant	1307:1325	arg1	status					1327:1332	testing oxidant/antioxidant status	1299:1332	testing oxidant/antioxidant status	1299:1332	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	4	3	theme	reduction	731:739	arg1	method					741:746	wet reduction method	727:746	wet reduction method of silver salt	727:761	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	1	4	theme	conventional	182:193	arg1	modalities					205:214	conventional treatment modalities	182:214	conventional treatment modalities	182:214	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	4	5	theme	loading	941:947	arg1	coating					837:843	oligomeric chitosan coating	817:843	oligomeric chitosan coating	817:843	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	5	theme	loading	941:947	arg1	conjugation					852:862	nisin conjugation	846:862	nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU	846:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	7	6	theme	therapeutic	1535:1545	arg1	potential					1547:1555	anticancer therapeutic potential	1524:1555	anticancer therapeutic potential of nisin and 5-florouracil	1524:1582	Interestingly, anticancer therapeutic potential of nisin and 5-florouracil was found to be enhanced in vivo when bound on single composite nanoconstruct.
35427747	6	7	theme	tumor	1380:1384	arg1	volume					1386:1391	mean tumor volume	1375:1391	mean tumor volume (68.34 %)	1375:1401	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	7	theme	tumor	1380:1384	arg1	%					1400:1400	68.34 %	1394:1400	68.34 %	1394:1400	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	5	8	theme	Biophysical	958:968	arg1	studies					987:993	Biophysical characterisation studies	958:993	Biophysical characterisation studies	958:993	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	2	9	theme	combination	307:317	arg1	therapy					319:325	combination therapy	307:325	combination therapy involving anticancer peptides and chemodrugs	307:370	To overcome such deficits, combination therapy involving anticancer peptides and chemodrugs is being considered as an attractive strategy.
35427747	2	9	theme	combination	307:317	arg1	strategy					409:416	an attractive strategy	395:416	an attractive strategy	395:416	To overcome such deficits, combination therapy involving anticancer peptides and chemodrugs is being considered as an attractive strategy.
35427747	1	10	theme	several	221:227	arg1	resistance					254:263	drug resistance	249:263	drug resistance	249:263	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	1	10	theme	several	221:227	arg1	limitations					229:239	several limitations	221:239	several limitations such as drug resistance, toxicity etc	221:277	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	6	11	theme	mean	1375:1378	arg1	volume					1386:1391	mean tumor volume	1375:1391	mean tumor volume (68.34 %)	1375:1401	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	11	theme	mean	1375:1378	arg1	%					1400:1400	68.34 %	1394:1400	68.34 %	1394:1400	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	3	12	theme	DMBA/TPA-induced	666:681	arg1	cancer					695:700	DMBA/TPA-induced murine skin cancer	666:700	DMBA/TPA-induced murine skin cancer	666:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	5	13	theme	developed	1013:1021	arg1	nanoconstruct					1023:1035	the developed nanoconstruct	1009:1035	the developed nanoconstruct	1009:1035	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	14	theme	marked	1350:1355	arg1	reduction					1357:1365	marked reduction	1350:1365	marked reduction	1350:1365	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	15	theme	tumor	1412:1416	arg1	burden					1418:1423	mean tumor burden	1407:1423	mean tumor burden (82.39 %)	1407:1433	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	15	theme	tumor	1412:1416	arg1	%					1432:1432	82.39 %	1426:1432	82.39 %	1426:1432	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	4	16	theme	salt	758:761	arg1	method					741:746	wet reduction method	727:746	wet reduction method of silver salt	727:761	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	0	17	theme	chitosan	71:78	arg1	nanoparticles					94:106	chitosan coated silver nanoparticles	71:106	chitosan coated silver nanoparticles against murine skin cancer	71:133	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	3	18	theme	skin	690:693	arg1	cancer					695:700	DMBA/TPA-induced murine skin cancer	666:700	DMBA/TPA-induced murine skin cancer	666:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	1	19	theme	health	154:159	arg1	Cancer					136:141	Cancer	136:141	Cancer	136:141	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	1	19	theme	health	154:159	arg1	worldwide					169:177	a major health concern worldwide	146:177	a major health concern worldwide	146:177	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	0	20	theme	silver	87:92	arg1	nanoparticles					94:106	chitosan coated silver nanoparticles	71:106	chitosan coated silver nanoparticles against murine skin cancer	71:133	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	4	21	theme	nisin	846:850	arg1	coating					837:843	oligomeric chitosan coating	817:843	oligomeric chitosan coating	817:843	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	21	theme	nisin	846:850	arg1	conjugation					852:862	nisin conjugation	846:862	nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU	846:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	6	22	theme	testing	1299:1305	arg1	status					1327:1332	testing oxidant/antioxidant status	1299:1332	testing oxidant/antioxidant status	1299:1332	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	0	23	theme	skin	123:126	arg1	cancer					128:133	murine skin cancer	116:133	murine skin cancer	116:133	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	5	24	theme	potential	1086:1094	arg1	of + 32.90 mV					1096:1108	zeta potential of + 32.90 mV	1081:1108	zeta potential of + 32.90 mV	1081:1108	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	24	theme	potential	1086:1094	arg1	420 nm					1073:1078	420 nm	1073:1078	420 nm	1073:1078	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	24	theme	potential	1086:1094	arg1	analysis					1142:1149	TEM analysis	1138:1149	TEM analysis	1138:1149	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	4	25	theme	oligomeric	817:826	arg1	coating					837:843	oligomeric chitosan coating	817:843	oligomeric chitosan coating	817:843	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	25	theme	oligomeric	817:826	arg1	conjugation					852:862	nisin conjugation	846:862	nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU	846:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	0	26	theme	Anticancer	0:9	arg1	potential					23:31	Anticancer therapeutic potential	0:31	Anticancer therapeutic potential of 5-fluorouracil and nisin	0:59	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	5	27	theme	absorption	1052:1061	arg1	maxima					1063:1068	UV-visible absorption maxima	1041:1068	UV-visible absorption maxima	1041:1068	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	28	theme	anticancer	1161:1170	arg1	potential					1184:1192	In vivo anticancer therapeutic potential	1153:1192	In vivo anticancer therapeutic potential	1153:1192	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	3	29	theme	silver	575:580	arg1	nanoparticles					582:594	oligomeric chitosan coated silver nanoparticles	548:594	oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer	548:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	6	30	theme	In	1153:1154	arg1	potential					1184:1192	In vivo anticancer therapeutic potential	1153:1192	In vivo anticancer therapeutic potential	1153:1192	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	0	31	theme	5-fluorouracil	36:49	arg1	potential					23:31	Anticancer therapeutic potential	0:31	Anticancer therapeutic potential of 5-fluorouracil and nisin	0:59	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	3	32	theme	nanoconstruct	520:532	arg1	potential					498:506	the anticancer potential	483:506	the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer	483:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	0	33	theme	nisin	55:59	arg1	potential					23:31	Anticancer therapeutic potential	0:31	Anticancer therapeutic potential of 5-fluorouracil and nisin	0:59	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	7	34	theme	single	1631:1636	arg1	nanoconstruct					1648:1660	single composite nanoconstruct	1631:1660	single composite nanoconstruct	1631:1660	Interestingly, anticancer therapeutic potential of nisin and 5-florouracil was found to be enhanced in vivo when bound on single composite nanoconstruct.
35427747	8	35	theme	single	1713:1718	arg1	platforms					1720:1728	synergetic single platforms	1702:1728	synergetic single platforms	1702:1728	The study forms a basis for developing synergetic single platforms against variety of cancers.
35427747	6	36	dep	assessed	1198:1205	arg1	status					1501:1506	improved oxidant/antioxidant status	1472:1506	improved oxidant/antioxidant status	1472:1506	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	36	dep	assessed	1198:1205	arg1	histoarchitecture					1450:1466	restored skin histoarchitecture	1436:1466	restored skin histoarchitecture	1436:1466	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	37	theme	analyses	1286:1293	arg1	terms					1210:1214	terms	1210:1214	terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction	1210:1365	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	38	theme	tumor	1219:1223	arg1	statistics					1225:1234	tumor statistics	1219:1234	tumor statistics	1219:1234	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	3	39	theme	anticancer	487:496	arg1	potential					498:506	the anticancer potential	483:506	the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer	483:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	4	40	theme	chitosan	889:896	arg1	oligomers					898:906	chitosan oligomers	889:906	chitosan oligomers (EDC/NHS chemistry)	889:926	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	40	theme	chitosan	889:896	arg1	chemistry					917:925	EDC/NHS chemistry	909:925	EDC/NHS chemistry	909:925	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	3	41	theme	chitosan	559:566	arg1	nanoparticles					582:594	oligomeric chitosan coated silver nanoparticles	548:594	oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer	548:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	5	42	theme	particle	1123:1130	arg1	420 nm					1073:1078	420 nm	1073:1078	420 nm	1073:1078	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	42	theme	particle	1123:1130	arg1	size					1132:1135	72.39 nm particle size	1114:1135	72.39 nm particle size	1114:1135	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	43	theme	mean	1407:1410	arg1	burden					1418:1423	mean tumor burden	1407:1423	mean tumor burden (82.39 %)	1407:1433	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	43	theme	mean	1407:1410	arg1	%					1432:1432	82.39 %	1426:1432	82.39 %	1426:1432	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	1	44	theme	toxicity	266:273	arg1	resistance					254:263	drug resistance	249:263	drug resistance	249:263	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	1	44	theme	toxicity	266:273	arg1	etc					275:277	toxicity etc	266:277	toxicity etc	266:277	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	7	45	theme	5-florouracil	1570:1582	arg1	potential					1547:1555	anticancer therapeutic potential	1524:1555	anticancer therapeutic potential of nisin and 5-florouracil	1524:1582	Interestingly, anticancer therapeutic potential of nisin and 5-florouracil was found to be enhanced in vivo when bound on single composite nanoconstruct.
35427747	2	46	theme	such	292:295	arg1	deficits					297:304	such deficits	292:304	such deficits	292:304	To overcome such deficits, combination therapy involving anticancer peptides and chemodrugs is being considered as an attractive strategy.
35427747	7	47	theme	nisin	1560:1564	arg1	potential					1547:1555	anticancer therapeutic potential	1524:1555	anticancer therapeutic potential of nisin and 5-florouracil	1524:1582	Interestingly, anticancer therapeutic potential of nisin and 5-florouracil was found to be enhanced in vivo when bound on single composite nanoconstruct.
35427747	4	48	theme	wet	727:729	arg1	method					741:746	wet reduction method	727:746	wet reduction method of silver salt	727:761	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	6	49	theme	oxidant/antioxidant	1481:1499	arg1	status					1501:1506	improved oxidant/antioxidant status	1472:1506	improved oxidant/antioxidant status	1472:1506	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	2	50	theme	anticancer	337:346	arg1	peptides					348:355	anticancer peptides	337:355	anticancer peptides	337:355	To overcome such deficits, combination therapy involving anticancer peptides and chemodrugs is being considered as an attractive strategy.
35427747	1	51	theme	treatment	195:203	arg1	modalities					205:214	conventional treatment modalities	182:214	conventional treatment modalities	182:214	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	4	52	theme	physical	932:939	arg1	loading					941:947	physical loading	932:947	physical loading of 5-FU	932:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	6	53	theme	restored	1436:1443	arg1	histoarchitecture					1450:1466	restored skin histoarchitecture	1436:1466	restored skin histoarchitecture	1436:1466	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	6	54	dep	terms	1210:1214	arg1	both					1367:1370	both	1367:1370	both	1367:1370	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	7	55	theme	anticancer	1524:1533	arg1	potential					1547:1555	anticancer therapeutic potential	1524:1555	anticancer therapeutic potential of nisin and 5-florouracil	1524:1582	Interestingly, anticancer therapeutic potential of nisin and 5-florouracil was found to be enhanced in vivo when bound on single composite nanoconstruct.
35427747	1	56	theme	major	148:152	arg1	Cancer					136:141	Cancer	136:141	Cancer	136:141	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	1	56	theme	major	148:152	arg1	worldwide					169:177	a major health concern worldwide	146:177	a major health concern worldwide	146:177	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	5	57	contain	had	1037:1039	arg1	nanoconstruct					1023:1035	the developed nanoconstruct	1009:1035	the developed nanoconstruct	1009:1035	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	57	contain	had	1037:1039	arg2	maxima					1063:1068	UV-visible absorption maxima	1041:1068	UV-visible absorption maxima	1041:1068	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	58	theme	improved	1472:1479	arg1	status					1501:1506	improved oxidant/antioxidant status	1472:1506	improved oxidant/antioxidant status	1472:1506	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	4	59	theme	silver	771:776	arg1	nanoparticles					778:790	silver nanoparticles	771:790	silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU	771:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	0	60	theme	coated	80:85	arg1	nanoparticles					94:106	chitosan coated silver nanoparticles	71:106	chitosan coated silver nanoparticles against murine skin cancer	71:133	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	3	61	theme	murine	683:688	arg1	cancer					695:700	DMBA/TPA-induced murine skin cancer	666:700	DMBA/TPA-induced murine skin cancer	666:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	5	62	theme	characterisation	970:985	arg1	studies					987:993	Biophysical characterisation studies	958:993	Biophysical characterisation studies	958:993	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	63	theme	status	1327:1332	arg1	terms					1210:1214	terms	1210:1214	terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction	1210:1365	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	4	64	theme	silver	751:756	arg1	salt					758:761	silver salt	751:761	silver salt	751:761	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	1	65	theme	concern	161:167	arg1	Cancer					136:141	Cancer	136:141	Cancer	136:141	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	1	65	theme	concern	161:167	arg1	worldwide					169:177	a major health concern worldwide	146:177	a major health concern worldwide	146:177	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	0	66	theme	murine	116:121	arg1	cancer					128:133	murine skin cancer	116:133	murine skin cancer	116:133	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	5	67	theme	zeta	1081:1084	arg1	of + 32.90 mV					1096:1108	zeta potential of + 32.90 mV	1081:1108	zeta potential of + 32.90 mV	1081:1108	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	67	theme	zeta	1081:1084	arg1	420 nm					1073:1078	420 nm	1073:1078	420 nm	1073:1078	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	67	theme	zeta	1081:1084	arg1	analysis					1142:1149	TEM analysis	1138:1149	TEM analysis	1138:1149	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	68	dep	In	1153:1154	arg1	vivo					1156:1159	vivo	1156:1159	vivo	1156:1159	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	0	69	theme	therapeutic	11:21	arg1	potential					23:31	Anticancer therapeutic potential	0:31	Anticancer therapeutic potential of 5-fluorouracil and nisin	0:59	Anticancer therapeutic potential of 5-fluorouracil and nisin co-loaded chitosan coated silver nanoparticles against murine skin cancer.
35427747	5	70	theme	UV-visible	1041:1050	arg1	maxima					1063:1068	UV-visible absorption maxima	1041:1068	UV-visible absorption maxima	1041:1068	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	71	theme	therapeutic	1172:1182	arg1	potential					1184:1192	In vivo anticancer therapeutic potential	1153:1192	In vivo anticancer therapeutic potential	1153:1192	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	2	72	theme	attractive	398:407	arg1	therapy					319:325	combination therapy	307:325	combination therapy involving anticancer peptides and chemodrugs	307:370	To overcome such deficits, combination therapy involving anticancer peptides and chemodrugs is being considered as an attractive strategy.
35427747	2	72	theme	attractive	398:407	arg1	strategy					409:416	an attractive strategy	395:416	an attractive strategy	395:416	To overcome such deficits, combination therapy involving anticancer peptides and chemodrugs is being considered as an attractive strategy.
35427747	6	73	dep	histopathological	1237:1253	arg1	microscopic					1274:1284	microscopic	1274:1284	microscopic	1274:1284	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	4	74	theme	chitosan	828:835	arg1	coating					837:843	oligomeric chitosan coating	817:843	oligomeric chitosan coating	817:843	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	74	theme	chitosan	828:835	arg1	conjugation					852:862	nisin conjugation	846:862	nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU	846:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	8	75	theme	synergetic	1702:1711	arg1	platforms					1720:1728	synergetic single platforms	1702:1728	synergetic single platforms	1702:1728	The study forms a basis for developing synergetic single platforms against variety of cancers.
35427747	4	76	theme	oligomers	898:906	arg1	coating					837:843	oligomeric chitosan coating	817:843	oligomeric chitosan coating	817:843	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	76	theme	oligomers	898:906	arg1	conjugation					852:862	nisin conjugation	846:862	nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU	846:955	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	6	77	theme	skin	1445:1448	arg1	histoarchitecture					1450:1466	restored skin histoarchitecture	1436:1466	restored skin histoarchitecture	1436:1466	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	5	78	theme	TEM	1138:1140	arg1	of + 32.90 mV					1096:1108	zeta potential of + 32.90 mV	1081:1108	zeta potential of + 32.90 mV	1081:1108	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	78	theme	TEM	1138:1140	arg1	analysis					1142:1149	TEM analysis	1138:1149	TEM analysis	1138:1149	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	6	79	theme	histopathological	1237:1253	arg1	analyses					1286:1293	histopathological, scanning electron microscopic analyses	1237:1293	histopathological, scanning electron microscopic analyses	1237:1293	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	3	80	theme	present	430:436	arg1	study					438:442	present study	430:442	present study	430:442	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	4	81	theme	EDC/NHS	909:915	arg1	oligomers					898:906	chitosan oligomers	889:906	chitosan oligomers (EDC/NHS chemistry)	889:926	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	4	81	theme	EDC/NHS	909:915	arg1	chemistry					917:925	EDC/NHS chemistry	909:925	EDC/NHS chemistry	909:925	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	7	82	theme	composite	1638:1646	arg1	nanoconstruct					1648:1660	single composite nanoconstruct	1631:1660	single composite nanoconstruct	1631:1660	Interestingly, anticancer therapeutic potential of nisin and 5-florouracil was found to be enhanced in vivo when bound on single composite nanoconstruct.
35427747	6	83	theme	statistics	1225:1234	arg1	terms					1210:1214	terms	1210:1214	terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction	1210:1365	In vivo anticancer therapeutic potential was assessed in terms of tumor statistics, histopathological, scanning electron microscopic analyses and testing oxidant/antioxidant status which exhibited marked reduction both in mean tumor volume (68.34 %) and mean tumor burden (82.39 %); restored skin histoarchitecture and improved oxidant/antioxidant status.
35427747	4	84	theme	deacetylated	867:878	arg1	units					880:884	deacetylated units	867:884	deacetylated units	867:884	It was fabricated using wet reduction method of silver salt to form silver nanoparticles followed sequentially by oligomeric chitosan coating, nisin conjugation to deacetylated units of chitosan oligomers (EDC/NHS chemistry) and physical loading of 5-FU.
35427747	3	85	theme	single	513:518	arg1	nanoconstruct					520:532	a single nanoconstruct	511:532	a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer	511:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35427747	1	86	theme	drug	249:252	arg1	resistance					254:263	drug resistance	249:263	drug resistance	249:263	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	1	86	theme	drug	249:252	arg1	etc					275:277	toxicity etc	266:277	toxicity etc	266:277	Cancer is a major health concern worldwide as conventional treatment modalities face several limitations such as drug resistance, toxicity etc.
35427747	5	87	theme	72.39 nm	1114:1121	arg1	420 nm					1073:1078	420 nm	1073:1078	420 nm	1073:1078	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	5	87	theme	72.39 nm	1114:1121	arg1	size					1132:1135	72.39 nm particle size	1114:1135	72.39 nm particle size	1114:1135	Biophysical characterisation studies revealed that the developed nanoconstruct had UV-visible absorption maxima at 420 nm, zeta potential of + 32.90 mV and 72.39 nm particle size (TEM analysis).
35427747	8	88	theme	cancers	1749:1755	arg1	cancers					1749:1755	cancers	1749:1755	cancers	1749:1755	The study forms a basis for developing synergetic single platforms against variety of cancers.
35427747	8	88	theme	cancers	1749:1755	arg1	variety					1738:1744	variety	1738:1744	variety of cancers	1738:1755	The study forms a basis for developing synergetic single platforms against variety of cancers.
35427747	3	89	theme	coated	568:573	arg1	nanoparticles					582:594	oligomeric chitosan coated silver nanoparticles	548:594	oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer	548:700	Therefore, present study developed, characterized and evaluated the anticancer potential of a single nanoconstruct comprising of oligomeric chitosan coated silver nanoparticles co-loaded with nisin and 5-florouracil (5-FU/nisin-CHI-AgNPs) against DMBA/TPA-induced murine skin cancer.
35804766	6	0	theme	multi-attribute	1017:1031	arg1	data					1033:1036	The multi-attribute data	1013:1036	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure	1013:1104	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	3	1	theme	meat	450:453	arg1	profiling					437:445	the profiling	433:445	the profiling of meat from different species	433:476	In this study, we use a glycomic approach for the profiling of meat from different species.
35804766	7	2	from	O-glycan	1327:1334	arg1	data					1317:1320	extract multi-attribute data	1293:1320	extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling	1293:1375	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	4	3	theme	chicken	682:688	arg1	samples					711:717	chicken, pork, and beef meat samples	682:717	chicken, pork, and beef meat samples	682:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	7	4	theme	multi-attribute	1301:1315	arg1	data					1317:1320	extract multi-attribute data	1293:1320	extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling	1293:1375	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	8	5	theme	established	1383:1393	arg1	methodology					1411:1421	This established glycoanalytical methodology	1378:1421	This established glycoanalytical methodology	1378:1421	This established glycoanalytical methodology could be extended to other high-value biotechnology industries for product authentication.
35804766	4	6	theme	high-resolution	565:579	arg1	spectrometry					652:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry	563:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples	563:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	4	6	theme	high-resolution	565:579	arg1	UPLC-FLR-MS					666:676	UPLC-FLR-MS	666:676	UPLC-FLR-MS	666:676	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	7	7	from	analysis	1349:1356	arg1	data					1317:1320	extract multi-attribute data	1293:1320	extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling	1293:1375	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	5	8	theme	high	902:905	arg1	mannose					907:913	high mannose	902:913	high mannose	902:913	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	7	9	theme	glycomics-based	1265:1279	arg1	workflow					1281:1288	a glycomics-based workflow	1263:1288	a glycomics-based workflow	1263:1288	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	3	10	from	species	470:476	arg1	profiling					437:445	the profiling	433:445	the profiling of meat from different species	433:476	In this study, we use a glycomic approach for the profiling of meat from different species.
35804766	6	11	theme	different	1150:1158	arg1	species					1160:1166	different species	1150:1166	different species	1150:1166	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	8	12	theme	glycoanalytical	1395:1409	arg1	methodology					1411:1421	This established glycoanalytical methodology	1378:1421	This established glycoanalytical methodology	1378:1421	This established glycoanalytical methodology could be extended to other high-value biotechnology industries for product authentication.
35804766	2	13	theme	profile	365:371	arg1	samples					378:384	and profile meat samples	361:384	samples	378:384	To overcome this problem, novel and robust quantitative methods are needed to accurately characterise and profile meat samples.
35804766	6	14	theme	structure	1096:1104	arg1	abundance					1056:1064	the abundance	1052:1064	the abundance of each O-glycan and N-glycan structure	1052:1104	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	4	15	from	spectrometry	652:663	arg1	samples					711:717	chicken, pork, and beef meat samples	682:717	chicken, pork, and beef meat samples	682:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	4	16	theme	chromatography-fluorescence-mass	619:650	arg1	spectrometry					652:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry	563:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples	563:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	4	16	theme	chromatography-fluorescence-mass	619:650	arg1	UPLC-FLR-MS					666:676	UPLC-FLR-MS	666:676	UPLC-FLR-MS	666:676	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	0	17	theme	Integrative	3:13	arg1	Approach					24:31	An Integrative Glycomic Approach	0:31	An Integrative Glycomic Approach for Quantitative Meat Species Profiling	0:71	An Integrative Glycomic Approach for Quantitative Meat Species Profiling.
35804766	6	18	from	species	1160:1166	arg1	separation					1121:1130	a clear separation	1113:1130	a clear separation between meat from different species	1113:1166	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	5	19	theme	chicken	792:798	arg1	profile					781:787	the distinct glycan profile	761:787	the distinct glycan profile of chicken, pork, and beef samples	761:822	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	4	20	theme	meat	706:709	arg1	samples					711:717	chicken, pork, and beef meat samples	682:717	chicken, pork, and beef meat samples	682:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	4	21	theme	O-glycan	496:503	arg1	analysis					505:512	an O-glycan analysis	493:512	an O-glycan analysis using LC-MS qTOF	493:529	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	8	22	theme	product	1490:1496	arg1	authentication					1498:1511	product authentication	1490:1511	product authentication	1490:1511	This established glycoanalytical methodology could be extended to other high-value biotechnology industries for product authentication.
35804766	1	23	from	species	133:139	arg1	meat					113:116	meat	113:116	meat from different species	113:139	It is estimated that food fraud, where meat from different species is deceitfully labelled or contaminated, has cost the global food industry around USD 6.2 to USD 40 billion annually.
35804766	1	24	theme	food	95:98	arg1	fraud					100:104	food fraud	95:104	food fraud	95:104	It is estimated that food fraud, where meat from different species is deceitfully labelled or contaminated, has cost the global food industry around USD 6.2 to USD 40 billion annually.
35804766	5	25	from	species	1004:1010	arg1	meat					984:987	meat	984:987	meat from different species	984:1010	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	3	26	theme	glycomic	411:418	arg1	approach					420:427	a glycomic approach	409:427	a glycomic approach for the profiling of meat from different species	409:476	In this study, we use a glycomic approach for the profiling of meat from different species.
35804766	0	27	theme	Glycomic	15:22	arg1	Approach					24:31	An Integrative Glycomic Approach	0:31	An Integrative Glycomic Approach for Quantitative Meat Species Profiling	0:71	An Integrative Glycomic Approach for Quantitative Meat Species Profiling.
35804766	4	28	theme	LC-MS	520:524	arg1	qTOF					526:529	LC-MS qTOF	520:529	LC-MS qTOF	520:529	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	8	29	theme	biotechnology	1461:1473	arg1	industries					1475:1484	other high-value biotechnology industries	1444:1484	other high-value biotechnology industries for product authentication	1444:1511	This established glycoanalytical methodology could be extended to other high-value biotechnology industries for product authentication.
35804766	6	30	theme	clear	1115:1119	arg1	separation					1121:1130	a clear separation	1113:1130	a clear separation between meat from different species	1113:1166	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	1	31	theme	different	123:131	arg1	species					133:139	different species	123:139	different species	123:139	It is estimated that food fraud, where meat from different species is deceitfully labelled or contaminated, has cost the global food industry around USD 6.2 to USD 40 billion annually.
35804766	0	32	theme	Quantitative	37:48	arg1	Profiling					63:71	Quantitative Meat Species Profiling	37:71	Quantitative Meat Species Profiling	37:71	An Integrative Glycomic Approach for Quantitative Meat Species Profiling.
35804766	4	33	theme	pork	691:694	arg1	samples					711:717	chicken, pork, and beef meat samples	682:717	chicken, pork, and beef meat samples	682:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	5	34	theme	beef	811:814	arg1	samples					816:822	beef samples	811:822	beef samples	811:822	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	4	35	theme	ultra-performance	594:610	arg1	spectrometry					652:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry	563:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples	563:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	4	35	theme	ultra-performance	594:610	arg1	UPLC-FLR-MS					666:676	UPLC-FLR-MS	666:676	UPLC-FLR-MS	666:676	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	7	36	theme	extract	1293:1299	arg1	data					1317:1320	extract multi-attribute data	1293:1320	extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling	1293:1375	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	4	37	theme	liquid	612:617	arg1	spectrometry					652:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry	563:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples	563:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	4	37	theme	liquid	612:617	arg1	UPLC-FLR-MS					666:676	UPLC-FLR-MS	666:676	UPLC-FLR-MS	666:676	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	5	38	theme	distinct	765:772	arg1	profile					781:787	the distinct glycan profile	761:787	the distinct glycan profile of chicken, pork, and beef samples	761:822	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	39	theme	samples	816:822	arg1	profile					781:787	the distinct glycan profile	761:787	the distinct glycan profile of chicken, pork, and beef samples	761:822	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	2	40	theme	meat	373:376	arg1	samples					378:384	and profile meat samples	361:384	samples	378:384	To overcome this problem, novel and robust quantitative methods are needed to accurately characterise and profile meat samples.
35804766	0	41	theme	Species	55:61	arg1	Profiling					63:71	Quantitative Meat Species Profiling	37:71	Quantitative Meat Species Profiling	37:71	An Integrative Glycomic Approach for Quantitative Meat Species Profiling.
35804766	5	42	theme	glycomic	735:742	arg1	approach					744:751	Our integrated glycomic approach	720:751	Our integrated glycomic approach	720:751	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	43	theme	different	994:1002	arg1	species					1004:1010	different species	994:1010	different species	994:1010	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	2	44	theme	quantitative	302:313	arg1	methods					315:321	novel and robust quantitative methods	285:321	novel and robust quantitative methods	285:321	To overcome this problem, novel and robust quantitative methods are needed to accurately characterise and profile meat samples.
35804766	0	45	theme	Meat	50:53	arg1	Profiling					63:71	Quantitative Meat Species Profiling	37:71	Quantitative Meat Species Profiling	37:71	An Integrative Glycomic Approach for Quantitative Meat Species Profiling.
35804766	1	46	dep	USD	234:236	arg1	billion					241:247	billion	241:247	USD 40 billion	234:247	It is estimated that food fraud, where meat from different species is deceitfully labelled or contaminated, has cost the global food industry around USD 6.2 to USD 40 billion annually.
35804766	8	47	theme	high-value	1450:1459	arg1	industries					1475:1484	other high-value biotechnology industries	1444:1484	other high-value biotechnology industries for product authentication	1444:1511	This established glycoanalytical methodology could be extended to other high-value biotechnology industries for product authentication.
35804766	7	48	theme	N-glycan	1340:1347	arg1	analysis					1349:1356	N-glycan analysis	1340:1356	N-glycan analysis	1340:1356	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	2	49	theme	robust	295:300	arg1	methods					315:321	novel and robust quantitative methods	285:321	novel and robust quantitative methods	285:321	To overcome this problem, novel and robust quantitative methods are needed to accurately characterise and profile meat samples.
35804766	6	50	theme	principal	1176:1184	arg1	analysis					1196:1203	principal component analysis	1176:1203	principal component analysis	1176:1203	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	8	51	theme	other	1444:1448	arg1	industries					1475:1484	other high-value biotechnology industries	1444:1484	other high-value biotechnology industries for product authentication	1444:1511	This established glycoanalytical methodology could be extended to other high-value biotechnology industries for product authentication.
35804766	7	52	theme	meat	1362:1365	arg1	profiling					1367:1375	meat profiling	1362:1375	meat profiling	1362:1375	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	4	53	theme	beef	701:704	arg1	meat					706:709	beef meat	701:709	beef meat	701:709	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	6	54	theme	N-glycan	1087:1094	arg1	structure					1096:1104	each O-glycan and N-glycan structure	1069:1104	structure	1096:1104	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	1	55	theme	food	202:205	arg1	industry					207:214	the global food industry	191:214	the global food industry around USD 6.2 to USD 40 billion	191:247	It is estimated that food fraud, where meat from different species is deceitfully labelled or contaminated, has cost the global food industry around USD 6.2 to USD 40 billion annually.
35804766	7	56	theme	workflow	1281:1288	arg1	use					1256:1258	the use	1252:1258	the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling	1252:1375	Altogether, we have successfully demonstrated the use of a glycomics-based workflow to extract multi-attribute data from O-glycan and N-glycan analysis for meat profiling.
35804766	2	57	theme	novel	285:289	arg1	methods					315:321	novel and robust quantitative methods	285:321	novel and robust quantitative methods	285:321	To overcome this problem, novel and robust quantitative methods are needed to accurately characterise and profile meat samples.
35804766	4	58	theme	N-glycan	539:546	arg1	analysis					548:555	an N-glycan analysis	536:555	an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples	536:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	5	59	theme	integrated	724:733	arg1	approach					744:751	Our integrated glycomic approach	720:751	Our integrated glycomic approach	720:751	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	1	60	theme	global	195:200	arg1	industry					207:214	the global food industry	191:214	the global food industry around USD 6.2 to USD 40 billion	191:247	It is estimated that food fraud, where meat from different species is deceitfully labelled or contaminated, has cost the global food industry around USD 6.2 to USD 40 billion annually.
35804766	6	61	theme	component	1186:1194	arg1	analysis					1196:1203	principal component analysis	1176:1203	principal component analysis	1176:1203	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
35804766	4	62	theme	non-targeted	581:592	arg1	spectrometry					652:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry	563:663	a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples	563:717	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	4	62	theme	non-targeted	581:592	arg1	UPLC-FLR-MS					666:676	UPLC-FLR-MS	666:676	UPLC-FLR-MS	666:676	This involves an O-glycan analysis using LC-MS qTOF, and an N-glycan analysis using a high-resolution non-targeted ultra-performance liquid chromatography-fluorescence-mass spectrometry (UPLC-FLR-MS) on chicken, pork, and beef meat samples.
35804766	3	63	theme	different	460:468	arg1	species					470:476	different species	460:476	different species	460:476	In this study, we use a glycomic approach for the profiling of meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	galactosylation					885:899	galactosylation	885:899	galactosylation	885:899	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	Neu5Gc					929:934	Neu5Gc	929:934	Neu5Gc	929:934	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	fucosylation					858:869	fucosylation	858:869	fucosylation	858:869	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	Neu5Ac					941:946	Neu5Ac	941:946	Neu5Ac	941:946	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	mannose					907:913	high mannose	902:913	high mannose	902:913	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	sialylation					872:882	sialylation	872:882	sialylation	872:882	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	α-galactose					916:926	α-galactose	916:926	α-galactose	916:926	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	64	theme	glycosylation	825:837	arg1	attributes					839:848	glycosylation attributes	825:848	glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac	825:946	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	65	theme	glycan	774:779	arg1	profile					781:787	the distinct glycan profile	761:787	the distinct glycan profile of chicken, pork, and beef samples	761:822	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	5	66	theme	pork	801:804	arg1	profile					781:787	the distinct glycan profile	761:787	the distinct glycan profile of chicken, pork, and beef samples	761:822	Our integrated glycomic approach reveals the distinct glycan profile of chicken, pork, and beef samples; glycosylation attributes such as fucosylation, sialylation, galactosylation, high mannose, α-galactose, Neu5Gc, and Neu5Ac are significantly different between meat from different species.
35804766	6	67	theme	O-glycan	1074:1081	arg1	structure					1096:1104	each O-glycan and N-glycan structure	1069:1104	structure	1096:1104	The multi-attribute data consisting of the abundance of each O-glycan and N-glycan structure allows a clear separation between meat from different species through principal component analysis.
36137988	11	0	from	increase	1575:1582	arg1	R.					1601:1602	R.	1601:1602	R.	1601:1602	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	11	0	from	increase	1575:1582	arg1	amount					1591:1596	the amount	1587:1596	the amount of R. rubra	1587:1608	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	4	1	theme	food	554:557	arg1	regimens					559:566	Five food regimens	549:566	Five food regimens	549:566	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	3	2	theme	oxidative	483:491	arg1	biomarkers					454:463	several biomarkers	446:463	several biomarkers (protein content, oxidative stress, energetic reserves and pigments)	446:532	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	2	theme	oxidative	483:491	arg1	stress					493:498	oxidative stress	483:498	oxidative stress	483:498	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	6	3	theme	carotenoids	981:991	arg1	composition					993:1003	a different C, N, and carotenoids composition	959:1003	a different C, N, and carotenoids composition	959:1003	RESULTS The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets.
36137988	14	4	theme	D.	2095:2096	arg1	magna					2098:2102	D. magna performance	2095:2114	D. magna performance	2095:2114	CONCLUSIONS Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance.
36137988	10	5	from	induction	1382:1390	arg1	peroxidation					1487:1498	lipid peroxidation	1481:1498	lipid peroxidation	1481:1498	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	10	5	from	induction	1382:1390	arg1	accumulation					1507:1518	an accumulation	1504:1518	an accumulation of glycogen and carotenoids	1504:1546	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	2	6	dep	Rhodopirellula	232:245	arg1	rubra					247:251	Rhodopirellula rubra	232:251	Rhodopirellula rubra	232:251	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	12	7	theme	significant	1699:1709	arg1	effects					1711:1717	No significant effects	1696:1717	No significant effects on Daphnia longevity (a 110-day assay)	1696:1756	No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested.
36137988	4	8	theme	R.	754:755	arg1	suspension					740:749	suspension	740:749	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga	614:820	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	9	theme	0.2	634:636	arg1	A					614:614	A	614:614	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	16	10	from	sufficient	2330:2339	arg1	diet					2279:2282	single diet	2272:2282	single diet	2272:2282	When in single diet, bacterial concentration is not nutritionally sufficient to raise D. magna even when in increased concentration.
36137988	6	11	theme	different	961:969	arg1	composition					993:1003	a different C, N, and carotenoids composition	959:1003	a different C, N, and carotenoids composition	959:1003	RESULTS The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets.
36137988	2	12	theme	food	282:285	arg1	source					287:292	a single or supplementary food source	256:292	a single or supplementary food source for Daphnia magna	256:310	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	2	12	theme	food	282:285	arg1	potential					198:206	the potential	194:206	the potential of the concentration of Rhodopirellula rubra	194:251	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	6	13	theme	mixed	1030:1034	arg1	diets					1036:1040	the mixed diets	1026:1040	the mixed diets	1026:1040	RESULTS The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets.
36137988	10	14	theme	oxidative	1395:1403	arg1	response					1412:1419	oxidative stress response	1395:1419	oxidative stress response (increased catalase and GST)	1395:1448	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	5	15	theme	D.	905:906	arg1	magna					908:912	D. magna	905:912	D. magna	905:912	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
36137988	0	16	from	rubra	49:53	arg1	Rich					12:15	Rich	12:15	Rich	12:15	Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?
36137988	4	17	dep	individual	591:600	arg1	A					614:614	A	614:614	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	17	dep	individual	591:600	arg1	replicates					602:611	replicates	602:611	replicates	602:611	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	17	dep	individual	591:600	arg1	suspension					698:707	suspension	698:707	suspension	698:707	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	11	18	theme	daphnid	1675:1681	arg1	performance					1683:1693	daphnid performance	1675:1693	daphnid performance	1675:1693	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	4	19	from	AU	728:729	arg1	A					614:614	A	614:614	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	1	20	theme	wild	108:111	arg1	organisms					121:129	the wild various organisms	104:129	the wild various organisms	104:129	BACKGROUND In the wild various organisms contribute to daphnids diet.
36137988	4	21	theme	arbitrary	670:678	arg1	AU					687:688	AU	687:688	AU	687:688	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	21	theme	arbitrary	670:678	arg1	units					680:684	0.2 arbitrary units	666:684	0.2 arbitrary units (AU)	666:689	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	6	22	from	increase	1014:1021	arg1	diets					1036:1040	the mixed diets	1026:1040	the mixed diets	1026:1040	RESULTS The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets.
36137988	13	23	theme	significant	1813:1823	arg1	percentage					1834:1843	a significant survival percentage	1811:1843	a significant survival percentage	1811:1843	However, a significant survival percentage and fertility (cumulative offspring is more than twice) was observed when D. magna was fed with the mixed diet.
36137988	10	24	theme	glycogen	1523:1530	arg1	peroxidation					1487:1498	lipid peroxidation	1481:1498	lipid peroxidation	1481:1498	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	10	24	theme	glycogen	1523:1530	arg1	accumulation					1507:1518	an accumulation	1504:1518	an accumulation of glycogen and carotenoids	1504:1546	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	9	25	theme	single	1285:1290	arg1	diets					1292:1296	bacterial single diets	1275:1296	bacterial single diets	1275:1296	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	1	26	theme	various	113:119	arg1	organisms					121:129	the wild various organisms	104:129	the wild various organisms	104:129	BACKGROUND In the wild various organisms contribute to daphnids diet.
36137988	4	27	theme	suspension	640:649	arg1	A					614:614	A	614:614	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	14	28	theme	diversified	2034:2044	arg1	food					2046:2049	a nutritional diversified food	2020:2049	a nutritional diversified food to the daphnids that induced differences in D. magna performance	2020:2114	CONCLUSIONS Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance.
36137988	4	29	dep	R.	796:797	arg1	rubra					799:803	R. rubra	796:803	R. rubra	796:803	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	9	30	from	parameters	1329:1338	arg1	content					1370:1376	the protein content	1358:1376	the protein content	1358:1376	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	4	31	theme	R.	654:655	arg1	suspension					640:649	suspension	640:649	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	0	32	theme	Daphnia	63:69	arg1	Performance					77:87	Daphnia magna Performance	63:87	Daphnia magna Performance	63:87	Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?
36137988	15	33	theme	bacterial	2232:2240	arg1	concentration					2242:2254	the bacterial concentration	2228:2254	the bacterial concentration tested	2228:2261	The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested.
36137988	16	34	theme	single	2272:2277	arg1	diet					2279:2282	single diet	2272:2282	single diet	2272:2282	When in single diet, bacterial concentration is not nutritionally sufficient to raise D. magna even when in increased concentration.
36137988	15	35	with	beneficial	2146:2155	arg1	increase					2163:2170	increase	2163:2170	increase in offspring	2163:2183	The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested.
36137988	15	36	theme	mixed	2121:2125	arg1	diets					2127:2131	The mixed diets	2117:2131	The mixed diets	2117:2131	The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested.
36137988	15	36	theme	mixed	2121:2125	arg1	beneficial					2146:2155	beneficial	2146:2155	beneficial	2146:2155	The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested.
36137988	9	37	theme	history	1321:1327	arg1	parameters					1329:1338	life history parameters	1316:1338	life history parameters	1316:1338	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	15	38	theme	magna	2192:2196	arg1	performance					2198:2208	D. magna performance	2189:2208	D. magna performance	2189:2208	The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested.
36137988	0	39	dep	Daphnia	63:69	arg1	magna					71:75	magna	71:75	magna	71:75	Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?
36137988	14	40	dep	magna	2098:2102	arg1	performance					2104:2114	performance	2104:2114	D. magna performance	2095:2114	CONCLUSIONS Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance.
36137988	3	41	theme	energetic	501:509	arg1	biomarkers					454:463	several biomarkers	446:463	several biomarkers (protein content, oxidative stress, energetic reserves and pigments)	446:532	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	41	theme	energetic	501:509	arg1	reserves					511:518	energetic reserves	501:518	energetic reserves	501:518	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	7	42	theme	magna	1098:1102	arg1	parameters					1117:1126	D. magna life-history parameters	1095:1126	D. magna life-history parameters	1095:1126	The results confirmed that the mixed diets improved D. magna life-history parameters.
36137988	3	43	dep	METHODS	313:319	arg1	assays					329:334	Feeding assays	321:334	METHODS Feeding assays	313:334	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	44	theme	life-history	418:429	arg1	parameters					431:440	life-history parameters	418:440	life-history parameters	418:440	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	4	45	theme	subcapitata	621:631	arg1	A					614:614	A	614:614	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	46	dep	A	614:614	arg1	suspension					740:749	suspension	740:749	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga	614:820	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	46	dep	A	614:614	arg1	0.2+A-suspension					776:791	0.2+A-suspension	776:791	0.2+A-suspension of R. rubra at 0.4 AU	776:813	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	46	dep	A	614:614	arg1	alga					817:820	alga	817:820	alga	817:820	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	4	46	dep	A	614:614	arg1	0.2+A					732:736	0.2+A	732:736	0.2+A	732:736	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	3	47	theme	chronic	388:394	arg1	assays					396:401	chronic assays	388:401	chronic assays (21 days)	388:411	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	47	theme	chronic	388:394	arg1	days					407:410	21 days	404:410	21 days	404:410	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	6	48	dep	RESULTS	929:935	arg1	diets					946:950	The five diets	937:950	RESULTS The five diets	929:950	RESULTS The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets.
36137988	3	49	theme	protein	466:472	arg1	biomarkers					454:463	several biomarkers	446:463	several biomarkers (protein content, oxidative stress, energetic reserves and pigments)	446:532	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	49	theme	protein	466:472	arg1	content					474:480	protein content	466:480	protein content	466:480	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	0	50	theme	Bacterium	24:32	arg1	rubra					49:53	the Bacterium Rhodopirellula rubra	20:53	the Bacterium Rhodopirellula rubra	20:53	Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?
36137988	13	51	theme	D.	1919:1920	arg1	magna					1922:1926	D. magna	1919:1926	D. magna	1919:1926	However, a significant survival percentage and fertility (cumulative offspring is more than twice) was observed when D. magna was fed with the mixed diet.
36137988	7	52	theme	mixed	1074:1078	arg1	diets					1080:1084	the mixed diets	1070:1084	the mixed diets	1070:1084	The results confirmed that the mixed diets improved D. magna life-history parameters.
36137988	9	53	dep	D.	1257:1258	arg1	magna					1260:1264	D. magna	1257:1264	D. magna fed with bacterial single diets, presented	1257:1307	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	5	54	theme	diets	859:863	arg1	effects					842:848	the effects	838:848	the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna	838:912	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
36137988	14	55	theme	nutritional	2022:2032	arg1	food					2046:2049	a nutritional diversified food	2020:2049	a nutritional diversified food to the daphnids that induced differences in D. magna performance	2020:2114	CONCLUSIONS Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance.
36137988	8	56	from	decrease	1131:1138	arg1	glycogen					1143:1150	glycogen	1143:1150	glycogen	1143:1150	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	12	57	theme	110-day	1743:1749	arg1	assay					1751:1755	a 110-day assay	1741:1755	a 110-day assay	1741:1755	No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested.
36137988	12	57	theme	110-day	1743:1749	arg1	longevity					1730:1738	Daphnia longevity	1722:1738	Daphnia longevity (a 110-day assay)	1722:1756	No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested.
36137988	16	58	theme	increased	2372:2380	arg1	concentration					2382:2394	increased concentration	2372:2394	increased concentration	2372:2394	When in single diet, bacterial concentration is not nutritionally sufficient to raise D. magna even when in increased concentration.
36137988	10	59	theme	lipid	1481:1485	arg1	peroxidation					1487:1498	lipid peroxidation	1481:1498	lipid peroxidation	1481:1498	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	8	60	from	increase	1161:1168	arg1	glycogen					1143:1150	glycogen	1143:1150	glycogen	1143:1150	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	13	61	theme	cumulative	1860:1869	arg1	offspring					1871:1879	cumulative offspring	1860:1879	cumulative offspring	1860:1879	However, a significant survival percentage and fertility (cumulative offspring is more than twice) was observed when D. magna was fed with the mixed diet.
36137988	2	62	theme	supplementary	268:280	arg1	source					287:292	a single or supplementary food source	256:292	a single or supplementary food source for Daphnia magna	256:310	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	2	62	theme	supplementary	268:280	arg1	potential					198:206	the potential	194:206	the potential of the concentration of Rhodopirellula rubra	194:251	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	12	63	theme	Daphnia	1722:1728	arg1	assay					1751:1755	a 110-day assay	1741:1755	a 110-day assay	1741:1755	No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested.
36137988	12	63	theme	Daphnia	1722:1728	arg1	longevity					1730:1738	Daphnia longevity	1722:1738	Daphnia longevity (a 110-day assay)	1722:1756	No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested.
36137988	10	64	from	decrease	1469:1476	arg1	peroxidation					1487:1498	lipid peroxidation	1481:1498	lipid peroxidation	1481:1498	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	10	64	from	decrease	1469:1476	arg1	accumulation					1507:1518	an accumulation	1504:1518	an accumulation of glycogen and carotenoids	1504:1546	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	2	65	theme	single	258:263	arg1	source					287:292	a single or supplementary food source	256:292	a single or supplementary food source for Daphnia magna	256:310	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	2	65	theme	single	258:263	arg1	potential					198:206	the potential	194:206	the potential of the concentration of Rhodopirellula rubra	194:251	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	15	66	from	increase	2163:2170	arg1	offspring					2175:2183	offspring	2175:2183	offspring	2175:2183	The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested.
36137988	6	67	theme	N	974:974	arg1	composition					993:1003	a different C, N, and carotenoids composition	959:1003	a different C, N, and carotenoids composition	959:1003	RESULTS The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets.
36137988	2	68	theme	Daphnia	298:304	arg1	magna					306:310	Daphnia magna	298:310	Daphnia magna	298:310	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	4	69	dep	R.	712:713	arg1	rubra					715:719	R. rubra	712:719	R. rubra	712:719	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	6	70	theme	C	971:971	arg1	composition					993:1003	a different C, N, and carotenoids composition	959:1003	a different C, N, and carotenoids composition	959:1003	RESULTS The five diets showed a different C, N, and carotenoids composition, with an increase in the mixed diets.
36137988	10	71	theme	carotenoids	1536:1546	arg1	peroxidation					1487:1498	lipid peroxidation	1481:1498	lipid peroxidation	1481:1498	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	10	71	theme	carotenoids	1536:1546	arg1	accumulation					1507:1518	an accumulation	1504:1518	an accumulation of glycogen and carotenoids	1504:1546	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	10	72	theme	stress	1405:1410	arg1	response					1412:1419	oxidative stress response	1395:1419	oxidative stress response (increased catalase and GST)	1395:1448	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	4	73	from	AU	812:813	arg1	0.2+A-suspension					776:791	0.2+A-suspension	776:791	0.2+A-suspension of R. rubra at 0.4 AU	776:813	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	2	74	theme	concentration	215:227	arg1	source					287:292	a single or supplementary food source	256:292	a single or supplementary food source for Daphnia magna	256:310	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	2	74	theme	concentration	215:227	arg1	potential					198:206	the potential	194:206	the potential of the concentration of Rhodopirellula rubra	194:251	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	5	75	dep	diets	859:863	arg1	0.2					869:871	0.2	869:871	0.2	869:871	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
36137988	5	75	dep	diets	859:863	arg1	0.2+A					878:882	0.2+A	878:882	0.2+A	878:882	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
36137988	5	75	dep	diets	859:863	arg1	A					866:866	A	866:866	A	866:866	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
36137988	5	75	dep	diets	859:863	arg1	diets					859:863	three diets	853:863	three diets (A, 0.2, and 0.2+A)	853:883	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
36137988	5	76	theme	magna	908:912	arg1	longevity					892:900	the longevity	888:900	the longevity of D. magna	888:912	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
36137988	15	77	theme	D.	2189:2190	arg1	performance					2198:2208	D. magna performance	2189:2208	D. magna performance	2189:2208	The mixed diets proved to be beneficial (with increase in offspring) on D. magna performance, independently of the bacterial concentration tested.
36137988	16	78	theme	D.	2350:2351	arg1	magna					2353:2357	D. magna	2350:2357	D. magna	2350:2357	When in single diet, bacterial concentration is not nutritionally sufficient to raise D. magna even when in increased concentration.
36137988	16	79	from	diet	2279:2282	arg1	sufficient					2330:2339	sufficient	2330:2339	sufficient	2330:2339	When in single diet, bacterial concentration is not nutritionally sufficient to raise D. magna even when in increased concentration.
36137988	8	80	theme	gluthione-S-transferase	1199:1221	arg1	decrease					1131:1138	A decrease	1129:1138	A decrease in glycogen	1129:1150	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	8	80	theme	gluthione-S-transferase	1199:1221	arg1	increase					1161:1168	the increase	1157:1168	the increase of haemoglobin, protein, and gluthione-S-transferase (GST)	1157:1227	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	9	81	theme	bacterial	1275:1283	arg1	diets					1292:1296	bacterial single diets	1275:1296	bacterial single diets	1275:1296	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	14	82	from	differences	2080:2090	arg1	magna					2098:2102	D. magna performance	2095:2114	D. magna performance	2095:2114	CONCLUSIONS Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance.
36137988	4	83	theme	R.	796:797	arg1	0.2+A-suspension					776:791	0.2+A-suspension	776:791	0.2+A-suspension of R. rubra at 0.4 AU	776:813	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	3	84	dep	biomarkers	454:463	arg1	biomarkers					454:463	several biomarkers	446:463	several biomarkers (protein content, oxidative stress, energetic reserves and pigments)	446:532	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	84	dep	biomarkers	454:463	arg1	pigments					524:531	pigments	524:531	pigments	524:531	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	84	dep	biomarkers	454:463	arg1	stress					493:498	oxidative stress	483:498	oxidative stress	483:498	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	84	dep	biomarkers	454:463	arg1	reserves					511:518	energetic reserves	501:518	energetic reserves	501:518	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	84	dep	biomarkers	454:463	arg1	content					474:480	protein content	466:480	protein content	466:480	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	4	85	theme	R.	712:713	arg1	A					614:614	A	614:614	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	13	86	theme	survival	1825:1832	arg1	percentage					1834:1843	a significant survival percentage	1811:1843	a significant survival percentage	1811:1843	However, a significant survival percentage and fertility (cumulative offspring is more than twice) was observed when D. magna was fed with the mixed diet.
36137988	2	87	theme	Rhodopirellula	232:245	arg1	concentration					215:227	the concentration	211:227	the concentration of Rhodopirellula rubra	211:251	This study, intendeds to evaluate the potential of the concentration of Rhodopirellula rubra as a single or supplementary food source for Daphnia magna.
36137988	13	88	theme	mixed	1945:1949	arg1	diet					1951:1954	the mixed diet	1941:1954	the mixed diet	1941:1954	However, a significant survival percentage and fertility (cumulative offspring is more than twice) was observed when D. magna was fed with the mixed diet.
36137988	9	89	from	decrease	1346:1353	arg1	content					1370:1376	the protein content	1358:1376	the protein content	1358:1376	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	3	90	theme	Feeding	321:327	arg1	assays					329:334	Feeding assays	321:334	METHODS Feeding assays	313:334	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	0	91	from	Rich	12:15	arg1	rubra					49:53	the Bacterium Rhodopirellula rubra	20:53	the Bacterium Rhodopirellula rubra	20:53	Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?
36137988	4	92	dep	R.	654:655	arg1	rubra					657:661	R. rubra	654:661	R. rubra	654:661	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	11	93	theme	D.	1622:1623	arg1	magna					1625:1629	D. magna	1622:1629	D. magna	1622:1629	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	4	94	from	0.2+alga	766:773	arg1	suspension					740:749	suspension	740:749	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga	614:820	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	1	95	theme	daphnids	145:152	arg1	diet					154:157	daphnids diet	145:157	daphnids diet	145:157	BACKGROUND In the wild various organisms contribute to daphnids diet.
36137988	9	96	theme	life	1316:1319	arg1	parameters					1329:1338	life history parameters	1316:1338	life history parameters	1316:1338	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	16	97	theme	bacterial	2285:2293	arg1	concentration					2295:2307	bacterial concentration	2285:2307	bacterial concentration	2285:2307	When in single diet, bacterial concentration is not nutritionally sufficient to raise D. magna even when in increased concentration.
36137988	11	98	dep	R.	1601:1602	arg1	rubra					1604:1608	R. rubra	1601:1608	R. rubra	1601:1608	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	14	99	theme	different	1995:2003	arg1	diets					2005:2009	different diets	1995:2009	different diets	1995:2009	CONCLUSIONS Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance.
36137988	10	100	theme	increased	1422:1430	arg1	catalase					1432:1439	catalase	1432:1439	catalase	1432:1439	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	4	101	dep	R.	754:755	arg1	rubra					757:761	R. rubra	754:761	R. rubra	754:761	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	7	102	theme	life-history	1104:1115	arg1	parameters					1117:1126	D. magna life-history parameters	1095:1126	D. magna life-history parameters	1095:1126	The results confirmed that the mixed diets improved D. magna life-history parameters.
36137988	14	103	theme	CONCLUSIONS	1957:1967	arg1	Results					1969:1975	CONCLUSIONS Results	1957:1975	CONCLUSIONS Results	1957:1975	CONCLUSIONS Results demonstrated that different diets provided a nutritional diversified food to the daphnids that induced differences in D. magna performance.
36137988	10	104	theme	response	1412:1419	arg1	decrease					1469:1476	a significant decrease	1455:1476	a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids	1455:1546	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	10	104	theme	response	1412:1419	arg1	induction					1382:1390	An induction	1379:1390	An induction of oxidative stress response (increased catalase and GST)	1379:1448	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	0	105	theme	Rich	12:15	arg1	Diet					7:10	a Diet	5:10	a Diet Rich in the Bacterium Rhodopirellula rubra	5:53	Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?
36137988	4	106	from	units	680:684	arg1	suspension					640:649	suspension	640:649	A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4	614:694	Five food regimens were conducted with 20 individual replicates (A - R. subcapitata; 0.2 - suspension of R. rubra at 0.2 arbitrary units (AU); 0.4 - suspension of R. rubra at 0.4 AU; 0.2+A - suspension of R. rubra at 0.2+alga; 0.2+A-suspension of R. rubra at 0.4 AU + alga).
36137988	7	107	theme	D.	1095:1096	arg1	parameters					1117:1126	D. magna life-history parameters	1095:1126	D. magna life-history parameters	1095:1126	The results confirmed that the mixed diets improved D. magna life-history parameters.
36137988	3	108	theme	standard	364:371	arg1	guidelines					373:382	standard guidelines	364:382	standard guidelines for chronic assays (21 days)	364:411	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	109	theme	several	446:452	arg1	biomarkers					454:463	several biomarkers	446:463	several biomarkers (protein content, oxidative stress, energetic reserves and pigments)	446:532	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	109	theme	several	446:452	arg1	content					474:480	protein content	466:480	protein content	466:480	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	109	theme	several	446:452	arg1	stress					493:498	oxidative stress	483:498	oxidative stress	483:498	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	3	109	theme	several	446:452	arg1	reserves					511:518	energetic reserves	501:518	energetic reserves	501:518	METHODS Feeding assays were performed according to standard guidelines for chronic assays (21 days), and life-history parameters and several biomarkers (protein content, oxidative stress, energetic reserves and pigments) were measured.
36137988	12	110	from	effects	1711:1717	arg1	assay					1751:1755	a 110-day assay	1741:1755	a 110-day assay	1741:1755	No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested.
36137988	12	110	from	effects	1711:1717	arg1	longevity					1730:1738	Daphnia longevity	1722:1738	Daphnia longevity (a 110-day assay)	1722:1756	No significant effects on Daphnia longevity (a 110-day assay) were observed among the three diets tested.
36137988	9	111	theme	protein	1362:1368	arg1	content					1370:1376	the protein content	1358:1376	the protein content	1358:1376	Furthermore, D. magna fed with bacterial single diets, presented worsen life history parameters and a decrease in the protein content.
36137988	8	112	theme	haemoglobin	1173:1183	arg1	decrease					1131:1138	A decrease	1129:1138	A decrease in glycogen	1129:1150	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	8	112	theme	haemoglobin	1173:1183	arg1	increase					1161:1168	the increase	1157:1168	the increase of haemoglobin, protein, and gluthione-S-transferase (GST)	1157:1227	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	0	113	theme	Rhodopirellula	34:47	arg1	rubra					49:53	the Bacterium Rhodopirellula rubra	20:53	the Bacterium Rhodopirellula rubra	20:53	Does a Diet Rich in the Bacterium Rhodopirellula rubra Improve Daphnia magna Performance?
36137988	11	114	dep	AU	1641:1642	arg1	AU					1651:1652	0.4 AU	1647:1652	0.2 AU to 0.4 AU	1637:1652	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	11	114	dep	AU	1641:1642	arg1	to					1644:1645	to	1644:1645	to	1644:1645	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	8	115	theme	protein	1186:1192	arg1	decrease					1131:1138	A decrease	1129:1138	A decrease in glycogen	1129:1150	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	8	115	theme	protein	1186:1192	arg1	increase					1161:1168	the increase	1157:1168	the increase of haemoglobin, protein, and gluthione-S-transferase (GST)	1157:1227	A decrease in glycogen, and the increase of haemoglobin, protein, and gluthione-S-transferase (GST) were observed.
36137988	10	116	theme	significant	1457:1467	arg1	decrease					1469:1476	a significant decrease	1455:1476	a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids	1455:1546	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	1	117	from	BACKGROUND	90:99	arg1	organisms					121:129	the wild various organisms	104:129	the wild various organisms	104:129	BACKGROUND In the wild various organisms contribute to daphnids diet.
36137988	13	118	dep	fertility	1849:1857	arg1	more					1884:1887	more	1884:1887	more	1884:1887	However, a significant survival percentage and fertility (cumulative offspring is more than twice) was observed when D. magna was fed with the mixed diet.
36137988	10	119	dep	response	1412:1419	arg1	GST					1445:1447	GST	1445:1447	GST	1445:1447	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	10	119	dep	response	1412:1419	arg1	catalase					1432:1439	catalase	1432:1439	catalase	1432:1439	An induction of oxidative stress response (increased catalase and GST), and a significant decrease in lipid peroxidation and an accumulation of glycogen and carotenoids were observed.
36137988	11	120	theme	R.	1601:1602	arg1	R.					1601:1602	R.	1601:1602	R.	1601:1602	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	11	120	theme	R.	1601:1602	arg1	amount					1591:1596	the amount	1587:1596	the amount of R. rubra	1587:1608	Overall, an increase in the amount of R. rubra provided to D. magna, from 0.2 AU to 0.4 AU, negatively impacted daphnid performance.
36137988	5	121	from	effects	842:848	arg1	longevity					892:900	the longevity	888:900	the longevity of D. magna	888:912	Additionally, the effects of three diets (A, 0.2, and 0.2+A) on the longevity of D. magna were assessed.
35290821	8	0	from	diffusion	1290:1298	arg1	biocatalysis					1303:1314	biocatalysis	1303:1314	biocatalysis	1303:1314	Silica shells can not only regulate the mass diffusion in biocatalysis but also enhance the long-term mechanical and chemical stability of the microcapsules.
35290821	13	1	from	progress	2230:2237	arg1	materials					2261:2269	cell-encapsulating materials	2242:2269	cell-encapsulating materials	2242:2269	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	7	2	theme	yeast	1232:1236	arg1	cells					1238:1242	the encapsulated yeast cells	1215:1242	the encapsulated yeast cells	1215:1242	Polydopamine enables to stimulate the biocatalytic productivity of the encapsulated yeast cells.
35290821	3	3	theme	enhanced	538:545	arg1	stability					547:555	enhanced stability	538:555	enhanced stability	538:555	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	13	4	theme	biocatalytic	2288:2299	arg1	industry					2322:2329	biocatalytic asymmetric synthesis industry	2288:2329	biocatalytic asymmetric synthesis industry	2288:2329	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	11	5	theme	alginate	1803:1810	arg1	microcapsules					1812:1824	pure alginate microcapsules	1798:1824	pure alginate microcapsules	1798:1824	The 24 h product yield of the cells encapsulated in the hybrid microcapsules shows 1.75 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	11	6	theme	product	1667:1673	arg1	yield					1675:1679	The 24 h product yield	1658:1679	The 24 h product yield of the cells encapsulated in the hybrid microcapsules	1658:1733	The 24 h product yield of the cells encapsulated in the hybrid microcapsules shows 1.75 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	13	7	theme	synthesis	2312:2320	arg1	industry					2322:2329	biocatalytic asymmetric synthesis industry	2288:2329	biocatalytic asymmetric synthesis industry	2288:2329	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	4	8	theme	alginate	687:694	arg1	microcapsules					714:726	hybrid alginate@polydopamine@SiO2 microcapsules	680:726	hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity	680:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	8	9	theme	mass	1285:1288	arg1	diffusion					1290:1298	the mass diffusion	1281:1298	the mass diffusion in biocatalysis	1281:1314	Silica shells can not only regulate the mass diffusion in biocatalysis but also enhance the long-term mechanical and chemical stability of the microcapsules.
35290821	4	10	theme	polydopamine	696:707	arg1	microcapsules					714:726	hybrid alginate@polydopamine@SiO2 microcapsules	680:726	hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity	680:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	11	11	theme	cells	1688:1692	arg1	yield					1675:1679	The 24 h product yield	1658:1679	The 24 h product yield of the cells encapsulated in the hybrid microcapsules	1658:1733	The 24 h product yield of the cells encapsulated in the hybrid microcapsules shows 1.75 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	5	12	theme	ionic	911:915	arg1	cross-linking					917:929	the ionic cross-linking	907:929	the ionic cross-linking of alginate	907:941	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	1	13	from	prospect	267:274	arg1	industry					313:320	chemical, pharmaceutical and fuel industry	279:320	chemical, pharmaceutical and fuel industry	279:320	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	3	14	theme	biocatalytic	584:595	arg1	productivity					597:608	low biocatalytic productivity	580:608	low biocatalytic productivity	580:608	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	5	15	theme	hybrid	872:877	arg1	microcapsules					879:891	The hybrid microcapsules	868:891	The hybrid microcapsules	868:891	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	4	16	theme	controlled	733:742	arg1	porosity					744:751	controlled porosity	733:751	controlled porosity	733:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	0	17	theme	-1-phenylethanol	123:138	arg1	biosynthesis					104:115	whole-cell based enantioselective biosynthesis	70:115	whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol	70:138	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	3	18	theme	poor	637:640	arg1	control					657:663	poor mass diffusion control	637:663	poor mass diffusion control	637:663	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	6	19	with	cells	1103:1107	arg1	environment					1135:1145	highly biocompatible environment	1114:1145	highly biocompatible environment	1114:1145	Alginate provides the encapsulated cells with highly biocompatible environment.
35290821	13	20	theme	significant	2218:2228	arg1	progress					2230:2237	a significant progress	2216:2237	a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry	2216:2329	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	5	21	theme	dopamine	966:973	arg1	monomers					975:982	dopamine monomers	966:982	dopamine monomers	966:982	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	12	22	theme	product	1853:1859	arg1	yield					1861:1865	the 24 h product yield	1844:1865	the 24 h product yield of the cells encapsulated in the hybrid microcapsules	1844:1919	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	12	22	theme	product	1853:1859	arg1	maintained					1929:1938	maintained	1929:1938	maintained	1929:1938	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	6	23	theme	encapsulated	1090:1101	arg1	cells					1103:1107	the encapsulated cells	1086:1107	the encapsulated cells with highly biocompatible environment	1086:1145	Alginate provides the encapsulated cells with highly biocompatible environment.
35290821	4	24	theme	SiO2	709:712	arg1	microcapsules					714:726	hybrid alginate@polydopamine@SiO2 microcapsules	680:726	hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity	680:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	13	25	theme	asymmetric	2301:2310	arg1	industry					2322:2329	biocatalytic asymmetric synthesis industry	2288:2329	biocatalytic asymmetric synthesis industry	2288:2329	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	3	26	theme	diffusion	647:655	arg1	control					657:663	poor mass diffusion control	637:663	poor mass diffusion control	637:663	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	10	27	theme	bioreduction	1575:1586	arg1	performance					1588:1598	asymmetric bioreduction performance	1564:1598	asymmetric bioreduction performance	1564:1598	The viability and asymmetric bioreduction performance of the cells encapsulated in microcapsules are evaluated.
35290821	11	28	theme	24 h	1662:1665	arg1	yield					1675:1679	The 24 h product yield	1658:1679	The 24 h product yield of the cells encapsulated in the hybrid microcapsules	1658:1733	The 24 h product yield of the cells encapsulated in the hybrid microcapsules shows 1.75 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	0	29	theme	whole-cell	70:79	arg1	biosynthesis					104:115	whole-cell based enantioselective biosynthesis	70:115	whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol	70:138	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	4	30	from	biosynthesis	812:823	arg1	acetophenone					854:865	acetophenone	854:865	acetophenone	854:865	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	0	31	theme	enantioselective	87:102	arg1	biosynthesis					104:115	whole-cell based enantioselective biosynthesis	70:115	whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol	70:138	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	10	32	theme	cells	1607:1611	arg1	viability					1550:1558	viability	1550:1558	viability	1550:1558	The viability and asymmetric bioreduction performance of the cells encapsulated in microcapsules are evaluated.
35290821	10	32	theme	cells	1607:1611	arg1	performance					1588:1598	asymmetric bioreduction performance	1564:1598	asymmetric bioreduction performance	1564:1598	The viability and asymmetric bioreduction performance of the cells encapsulated in microcapsules are evaluated.
35290821	13	33	theme	cells	2188:2192	arg1	activity					2132:2139	asymmetric biocatalytic activity	2108:2139	asymmetric biocatalytic activity	2108:2139	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	13	33	theme	cells	2188:2192	arg1	reusability					2156:2166	reusability	2156:2166	reusability	2156:2166	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	13	33	theme	cells	2188:2192	arg1	stability					2142:2150	stability	2142:2150	stability	2142:2150	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	12	34	theme	alginate	2003:2010	arg1	microcapsules					2012:2024	pure alginate microcapsules	1998:2024	pure alginate microcapsules	1998:2024	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	5	35	theme	uniform-sized	1032:1044	arg1	nanoparticles					1053:1065	uniform-sized silica nanoparticles	1032:1065	uniform-sized silica nanoparticles	1032:1065	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	0	36	theme	Alginate	0:7	arg1	SiO2					22:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	13	37	dep	activity	2132:2139	arg1	the					2104:2106	the	2104:2106	the	2104:2106	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	9	38	dep	morphology	1407:1416	arg1	The					1403:1405	The	1403:1405	The	1403:1405	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	12	39	dep	times	1946:1950	arg1	higher					1952:1957	higher	1952:1957	higher	1952:1957	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	0	40	theme	polydopamine	9:20	arg1	SiO2					22:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	5	41	theme	protamine-assisted	992:1009	arg1	packing					1021:1027	the protamine-assisted colloidal packing	988:1027	the protamine-assisted colloidal packing of uniform-sized silica nanoparticles	988:1065	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	6	42	theme	biocompatible	1121:1133	arg1	environment					1135:1145	highly biocompatible environment	1114:1145	highly biocompatible environment	1114:1145	Alginate provides the encapsulated cells with highly biocompatible environment.
35290821	2	43	theme	low	406:408	arg1	productivity					410:421	the low productivity	402:421	the low productivity	402:421	However, several problems still limit its wide applications, mainly concerning the low productivity and poor stability.
35290821	10	44	dep	viability	1550:1558	arg1	The					1546:1548	The	1546:1548	The	1546:1548	The viability and asymmetric bioreduction performance of the cells encapsulated in microcapsules are evaluated.
35290821	9	45	theme	molecular	1466:1474	arg1	accessibility					1476:1488	molecular accessibility	1466:1488	molecular accessibility	1466:1488	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	12	46	theme	2	1944:1944	arg1	times					1946:1950	times	1946:1950	times	1946:1950	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	3	47	theme	alginate	507:514	arg1	hydrogels					516:524	the most-commonly-used alginate hydrogels	484:524	the most-commonly-used alginate hydrogels	484:524	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	9	48	theme	hybrid	1497:1502	arg1	microcapsules					1504:1516	the hybrid microcapsules	1493:1516	the hybrid microcapsules	1493:1516	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	5	49	theme	nanoparticles	1053:1065	arg1	polymerization					948:961	the polymerization	944:961	the polymerization of dopamine monomers	944:982	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	5	49	theme	nanoparticles	1053:1065	arg1	cross-linking					917:929	the ionic cross-linking	907:929	the ionic cross-linking of alginate	907:941	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	5	49	theme	nanoparticles	1053:1065	arg1	packing					1021:1027	the protamine-assisted colloidal packing	988:1027	the protamine-assisted colloidal packing of uniform-sized silica nanoparticles	988:1065	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	4	50	theme	@	708:708	arg1	microcapsules					714:726	hybrid alginate@polydopamine@SiO2 microcapsules	680:726	hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity	680:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	13	51	theme	biocatalytic	2119:2130	arg1	activity					2132:2139	asymmetric biocatalytic activity	2108:2139	asymmetric biocatalytic activity	2108:2139	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	2	52	theme	wide	365:368	arg1	applications					370:381	its wide applications	361:381	its wide applications	361:381	However, several problems still limit its wide applications, mainly concerning the low productivity and poor stability.
35290821	7	53	theme	cells	1238:1242	arg1	productivity					1199:1210	the biocatalytic productivity	1182:1210	the biocatalytic productivity of the encapsulated yeast cells	1182:1242	Polydopamine enables to stimulate the biocatalytic productivity of the encapsulated yeast cells.
35290821	7	54	theme	encapsulated	1219:1230	arg1	cells					1238:1242	the encapsulated yeast cells	1215:1242	the encapsulated yeast cells	1215:1242	Polydopamine enables to stimulate the biocatalytic productivity of the encapsulated yeast cells.
35290821	11	55	theme	higher	1752:1757	arg1	times					1746:1750	1.75 times	1741:1750	1.75 times higher than that of the cells encapsulated in pure alginate microcapsules	1741:1824	The 24 h product yield of the cells encapsulated in the hybrid microcapsules shows 1.75 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	8	56	theme	Silica	1245:1250	arg1	shells					1252:1257	Silica shells	1245:1257	Silica shells	1245:1257	Silica shells can not only regulate the mass diffusion in biocatalysis but also enhance the long-term mechanical and chemical stability of the microcapsules.
35290821	1	57	theme	great	261:265	arg1	prospect					267:274	a great prospect	259:274	a great prospect in chemical, pharmaceutical and fuel industry	259:320	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	12	58	theme	hybrid	1900:1905	arg1	microcapsules					1907:1919	the hybrid microcapsules	1896:1919	the hybrid microcapsules	1896:1919	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	4	59	with	microcapsules	714:726	arg1	porosity					744:751	controlled porosity	733:751	controlled porosity	733:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	7	60	theme	biocatalytic	1186:1197	arg1	productivity					1199:1210	the biocatalytic productivity	1182:1210	the biocatalytic productivity of the encapsulated yeast cells	1182:1242	Polydopamine enables to stimulate the biocatalytic productivity of the encapsulated yeast cells.
35290821	4	61	theme	hybrid	680:685	arg1	microcapsules					714:726	hybrid alginate@polydopamine@SiO2 microcapsules	680:726	hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity	680:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	11	62	theme	pure	1798:1801	arg1	microcapsules					1812:1824	pure alginate microcapsules	1798:1824	pure alginate microcapsules	1798:1824	The 24 h product yield of the cells encapsulated in the hybrid microcapsules shows 1.75 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	1	63	theme	high	179:182	arg1	enantioselectivity					184:201	its high enantioselectivity	175:201	its high enantioselectivity	175:201	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	12	64	theme	cells	1874:1878	arg1	yield					1861:1865	the 24 h product yield	1844:1865	the 24 h product yield of the cells encapsulated in the hybrid microcapsules	1844:1919	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	12	64	theme	cells	1874:1878	arg1	maintained					1929:1938	maintained	1929:1938	maintained	1929:1938	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	8	65	theme	mechanical	1347:1356	arg1	stability					1371:1379	the long-term mechanical and chemical stability	1333:1379	the long-term mechanical and chemical stability of the microcapsules	1333:1400	Silica shells can not only regulate the mass diffusion in biocatalysis but also enhance the long-term mechanical and chemical stability of the microcapsules.
35290821	3	66	theme	low	580:582	arg1	productivity					597:608	low biocatalytic productivity	580:608	low biocatalytic productivity	580:608	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	8	67	theme	chemical	1362:1369	arg1	stability					1371:1379	the long-term mechanical and chemical stability	1333:1379	the long-term mechanical and chemical stability of the microcapsules	1333:1400	Silica shells can not only regulate the mass diffusion in biocatalysis but also enhance the long-term mechanical and chemical stability of the microcapsules.
35290821	4	68	theme	@	695:695	arg1	microcapsules					714:726	hybrid alginate@polydopamine@SiO2 microcapsules	680:726	hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity	680:751	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	3	69	theme	long-term	611:619	arg1	reusability					621:631	long-term reusability	611:631	long-term reusability	611:631	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	4	70	from	acetophenone	854:865	arg1	- 1-phenylethonal					831:847	(S)- 1-phenylethonal	828:847	(S)- 1-phenylethonal from acetophenone	828:865	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	4	70	from	acetophenone	854:865	arg1	biosynthesis					812:823	the asymmetric biosynthesis	797:823	the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone	797:865	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	8	71	theme	microcapsules	1388:1400	arg1	stability					1371:1379	the long-term mechanical and chemical stability	1333:1379	the long-term mechanical and chemical stability of the microcapsules	1333:1400	Silica shells can not only regulate the mass diffusion in biocatalysis but also enhance the long-term mechanical and chemical stability of the microcapsules.
35290821	13	72	theme	hybrid	2042:2047	arg1	microcapsules					2049:2061	the hybrid microcapsules	2038:2061	the hybrid microcapsules designed in this study	2038:2084	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	13	73	theme	cell-encapsulating	2242:2259	arg1	materials					2261:2269	cell-encapsulating materials	2242:2269	cell-encapsulating materials	2242:2269	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	3	74	theme	mass	642:645	arg1	control					657:663	poor mass diffusion control	637:663	poor mass diffusion control	637:663	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	1	75	theme	Whole-cell	141:150	arg1	biocatalysis					152:163	Whole-cell biocatalysis	141:163	Whole-cell biocatalysis	141:163	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	12	76	theme	24 h	1848:1851	arg1	yield					1861:1865	the 24 h product yield	1844:1865	the 24 h product yield of the cells encapsulated in the hybrid microcapsules	1844:1919	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	12	76	theme	24 h	1848:1851	arg1	maintained					1929:1938	maintained	1929:1938	maintained	1929:1938	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	1	77	theme	mild	229:232	arg1	condition					243:251	mild reaction condition	229:251	mild reaction condition	229:251	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	0	78	theme	based	81:85	arg1	biosynthesis					104:115	whole-cell based enantioselective biosynthesis	70:115	whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol	70:138	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	5	79	theme	alginate	934:941	arg1	polymerization					948:961	the polymerization	944:961	the polymerization of dopamine monomers	944:982	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	5	79	theme	alginate	934:941	arg1	cross-linking					917:929	the ionic cross-linking	907:929	the ionic cross-linking of alginate	907:941	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	5	79	theme	alginate	934:941	arg1	packing					1021:1027	the protamine-assisted colloidal packing	988:1027	the protamine-assisted colloidal packing of uniform-sized silica nanoparticles	988:1065	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	0	80	theme	S	121:121	arg1	-1-phenylethanol					123:138	(S)-1-phenylethanol	120:138	(S)-1-phenylethanol	120:138	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	10	81	theme	asymmetric	1564:1573	arg1	performance					1588:1598	asymmetric bioreduction performance	1564:1598	asymmetric bioreduction performance	1564:1598	The viability and asymmetric bioreduction performance of the cells encapsulated in microcapsules are evaluated.
35290821	5	82	theme	colloidal	1011:1019	arg1	packing					1021:1027	the protamine-assisted colloidal packing	988:1027	the protamine-assisted colloidal packing of uniform-sized silica nanoparticles	988:1065	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	13	83	theme	encapsulated	2175:2186	arg1	cells					2188:2192	the encapsulated cells	2171:2192	the encapsulated cells	2171:2192	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	1	84	theme	environment	204:214	arg1	friendly					216:223	environment friendly	204:223	environment friendly	204:223	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	4	85	theme	asymmetric	801:810	arg1	biosynthesis					812:823	the asymmetric biosynthesis	797:823	the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone	797:865	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	0	86	theme	@	8:8	arg1	SiO2					22:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	9	87	theme	chemical	1430:1437	arg1	composition					1439:1449	chemical composition	1430:1449	chemical composition	1430:1449	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	5	88	theme	monomers	975:982	arg1	polymerization					948:961	the polymerization	944:961	the polymerization of dopamine monomers	944:982	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	5	88	theme	monomers	975:982	arg1	cross-linking					917:929	the ionic cross-linking	907:929	the ionic cross-linking of alginate	907:941	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	5	88	theme	monomers	975:982	arg1	packing					1021:1027	the protamine-assisted colloidal packing	988:1027	the protamine-assisted colloidal packing of uniform-sized silica nanoparticles	988:1065	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	8	89	theme	long-term	1337:1345	arg1	stability					1371:1379	the long-term mechanical and chemical stability	1333:1379	the long-term mechanical and chemical stability of the microcapsules	1333:1400	Silica shells can not only regulate the mass diffusion in biocatalysis but also enhance the long-term mechanical and chemical stability of the microcapsules.
35290821	0	90	theme	@	21:21	arg1	SiO2					22:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2	0:25	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	1	91	theme	chemical	279:286	arg1	industry					313:320	chemical, pharmaceutical and fuel industry	279:320	chemical, pharmaceutical and fuel industry	279:320	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	2	92	theme	poor	427:430	arg1	stability					432:440	poor stability	427:440	poor stability	427:440	However, several problems still limit its wide applications, mainly concerning the low productivity and poor stability.
35290821	4	93	theme	yeast	781:785	arg1	cells					787:791	yeast cells	781:791	yeast cells	781:791	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	1	94	theme	pharmaceutical	289:302	arg1	industry					313:320	chemical, pharmaceutical and fuel industry	279:320	chemical, pharmaceutical and fuel industry	279:320	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	12	95	theme	pure	1998:2001	arg1	microcapsules					2012:2024	pure alginate microcapsules	1998:2024	pure alginate microcapsules	1998:2024	After 6 batches, the 24 h product yield of the cells encapsulated in the hybrid microcapsules is well maintained and 2 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	4	96	theme	- 1-phenylethonal	831:847	arg1	biosynthesis					812:823	the asymmetric biosynthesis	797:823	the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone	797:865	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
35290821	1	97	theme	reaction	234:241	arg1	condition					243:251	mild reaction condition	229:251	mild reaction condition	229:251	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	0	98	theme	controlled	46:55	arg1	porosity					57:64	controlled porosity	46:64	controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol	46:138	Alginate@polydopamine@SiO2 microcapsules with controlled porosity for whole-cell based enantioselective biosynthesis of (S)-1-phenylethanol.
35290821	1	99	theme	fuel	308:311	arg1	industry					313:320	chemical, pharmaceutical and fuel industry	279:320	chemical, pharmaceutical and fuel industry	279:320	Whole-cell biocatalysis, owing to its high enantioselectivity, environment friendly and mild reaction condition, show a great prospect in chemical, pharmaceutical and fuel industry.
35290821	11	100	theme	hybrid	1714:1719	arg1	microcapsules					1721:1733	the hybrid microcapsules	1710:1733	the hybrid microcapsules	1710:1733	The 24 h product yield of the cells encapsulated in the hybrid microcapsules shows 1.75 times higher than that of the cells encapsulated in pure alginate microcapsules.
35290821	2	101	theme	several	332:338	arg1	problems					340:347	several problems	332:347	several problems	332:347	However, several problems still limit its wide applications, mainly concerning the low productivity and poor stability.
35290821	5	102	theme	silica	1046:1051	arg1	nanoparticles					1053:1065	uniform-sized silica nanoparticles	1032:1065	uniform-sized silica nanoparticles	1032:1065	The hybrid microcapsules are formed by the ionic cross-linking of alginate, the polymerization of dopamine monomers and the protamine-assisted colloidal packing of uniform-sized silica nanoparticles.
35290821	13	103	theme	asymmetric	2108:2117	arg1	activity					2132:2139	asymmetric biocatalytic activity	2108:2139	asymmetric biocatalytic activity	2108:2139	Therefore, the hybrid microcapsules designed in this study enable to enhance the asymmetric biocatalytic activity, stability and reusability of the encapsulated cells, thus contributing to a significant progress in cell-encapsulating materials to be applied in biocatalytic asymmetric synthesis industry.
35290821	3	104	theme	most-commonly-used	488:505	arg1	hydrogels					516:524	the most-commonly-used alginate hydrogels	484:524	the most-commonly-used alginate hydrogels	484:524	Although the biocatalyst encapsulated in the most-commonly-used alginate hydrogels demonstrate enhanced stability, it still suffers from low biocatalytic productivity, long-term reusability and poor mass diffusion control.
35290821	9	105	theme	microcapsules	1504:1516	arg1	stability					1452:1460	stability	1452:1460	stability	1452:1460	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	9	105	theme	microcapsules	1504:1516	arg1	accessibility					1476:1488	molecular accessibility	1466:1488	molecular accessibility	1466:1488	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	9	105	theme	microcapsules	1504:1516	arg1	composition					1439:1449	chemical composition	1430:1449	chemical composition	1430:1449	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	9	105	theme	microcapsules	1504:1516	arg1	morphology					1407:1416	morphology	1407:1416	morphology	1407:1416	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	9	105	theme	microcapsules	1504:1516	arg1	structure					1419:1427	structure	1419:1427	structure	1419:1427	The morphology, structure, chemical composition, stability and molecular accessibility of the hybrid microcapsules are investigated in detail.
35290821	4	106	theme	S	829:829	arg1	- 1-phenylethonal					831:847	(S)- 1-phenylethonal	828:847	(S)- 1-phenylethonal from acetophenone	828:865	In this work, hybrid alginate@polydopamine@SiO2 microcapsules with controlled porosity are designed to encapsulate yeast cells for the asymmetric biosynthesis of (S)- 1-phenylethonal from acetophenone.
36367514	6	0	theme	unique	929:934	arg1	spectra					968:974	uniform, unique and reproducible MALDI-TOF mass spectra	920:974	uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum	920:1031	They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum.
36367514	4	1	theme	small	554:558	arg1	colonies					579:586	small Campylobacter-like colonies	554:586	small Campylobacter-like colonies	554:586	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	9	2	theme	ANI	1419:1421	arg1	species					1410:1416	the previously described Helicobacter species	1372:1416	the previously described Helicobacter species (ANI ≤90.6 %)	1372:1430	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	2	theme	ANI	1419:1421	arg1	%					1429:1429	ANI ≤90.6 %	1419:1429	ANI ≤90.6 %	1419:1429	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	8	3	theme	≥99.8 	1182:1187	arg1	similarity					1170:1179	a high similarity	1163:1179	a high similarity (≥99.8 % identity) to Helicobacter canadensis	1163:1225	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	8	3	theme	≥99.8 	1182:1187	arg1	identity					1190:1197	≥99.8 % identity	1182:1197	≥99.8 % identity	1182:1197	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	6	4	theme	uniform	920:926	arg1	spectra					968:974	uniform, unique and reproducible MALDI-TOF mass spectra	920:974	uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum	920:1031	They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum.
36367514	4	5	theme	standard	374:381	arg1	procedure					393:401	the standard ISO 10272 procedure	370:401	the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time	370:481	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	2	6	theme	harmonized	257:266	arg1	monitoring					268:277	the EFSA-coordinated harmonized monitoring	236:277	the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	236:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	4	7	theme	10272	387:391	arg1	procedure					393:401	the standard ISO 10272 procedure	370:401	the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time	370:481	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	4	8	theme	Skirow	653:658	arg1	plates					665:670	modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates	592:670	plates	665:670	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	6	9	theme	biochemical	894:904	arg1	tests					906:910	the standard-prescribed biochemical tests	870:910	the standard-prescribed biochemical tests	870:910	They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum.
36367514	9	10	theme	average	1237:1243	arg1	ANI					1266:1268	ANI	1266:1268	ANI	1266:1268	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	10	theme	average	1237:1243	arg1	identity					1256:1263	Pairwise average nucleotide identity	1228:1263	Pairwise average nucleotide identity (ANI) values	1228:1276	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	2	11	theme	resistance	296:305	arg1	monitoring					268:277	the EFSA-coordinated harmonized monitoring	236:277	the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	236:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	12	12	theme	strain	1742:1747	arg1	76053T					1781:1786	= DSM 113688T = CCUG 76053T	1760:1786	= DSM 113688T = CCUG 76053T	1760:1786	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	12	12	theme	strain	1742:1747	arg1	11154-15T					1749:1757	strain 11154-15T	1742:1757	strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain	1742:1806	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	4	13	theme	ISO	383:385	arg1	procedure					393:401	the standard ISO 10272 procedure	370:401	the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time	370:481	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	7	14	theme	whole-genome	1093:1104	arg1	sequencing					1106:1115	whole-genome sequencing	1093:1115	whole-genome sequencing	1093:1115	Three strains (11154-15T, 14348-15 and 16470-15) underwent whole-genome sequencing.
36367514	9	15	theme	identity	1256:1263	arg1	values					1271:1276	Pairwise average nucleotide identity (ANI) values	1228:1276	Pairwise average nucleotide identity (ANI) values	1228:1276	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	4	16	theme	thermotolerant	424:437	arg1	Campylobacter					439:451	thermotolerant Campylobacter	424:451	thermotolerant Campylobacter	424:451	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	6	17	theme	mass	963:966	arg1	spectra					968:974	uniform, unique and reproducible MALDI-TOF mass spectra	920:974	uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum	920:1031	They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum.
36367514	6	18	theme	reproducible	940:951	arg1	spectra					968:974	uniform, unique and reproducible MALDI-TOF mass spectra	920:974	uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum	920:1031	They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum.
36367514	2	19	from	monitoring	268:277	arg1	sp					324:325	Campylobacter sp	310:325	Campylobacter sp	310:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	1	20	dep	Sus	80:82	arg1	domesticus					91:100	domesticus	91:100	Sus scrofa domesticus	80:100	nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus).
36367514	4	21	theme	small	494:498	arg1	colonies					522:529	small, grey, moist and flat colonies	494:529	small, grey, moist and flat colonies	494:529	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	10	22	theme	core	1437:1440	arg1	phylogeny					1455:1463	The core genome-based phylogeny	1433:1463	The core genome-based phylogeny	1433:1463	The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis.
36367514	1	23	dep	pigs	74:77	arg1	Sus					80:82	Sus	80:82	Sus	80:82	nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus).
36367514	4	24	theme	grey	501:504	arg1	colonies					522:529	small, grey, moist and flat colonies	494:529	small, grey, moist and flat colonies	494:529	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	4	25	theme	incubation	467:476	arg1	time					478:481	extended incubation time	458:481	extended incubation time	458:481	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	1	26	theme	domestic	65:72	arg1	pigs					74:77	domestic pigs	65:77	domestic pigs (Sus scrofa domesticus)	65:101	nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus).
36367514	4	27	with	procedure	393:401	arg1	time					478:481	extended incubation time	458:481	extended incubation time	458:481	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	0	28	theme	Helicobacter	0:11	arg1	sp					22:23	Helicobacter colisuis sp	0:23	Helicobacter colisuis sp.	0:24	Helicobacter colisuis sp.
36367514	12	29	theme	DSM	1762:1764	arg1	76053T					1781:1786	= DSM 113688T = CCUG 76053T	1760:1786	= DSM 113688T = CCUG 76053T	1760:1786	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	12	29	theme	DSM	1762:1764	arg1	11154-15T					1749:1757	strain 11154-15T	1742:1757	strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain	1742:1806	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	6	30	theme	standard-prescribed	874:892	arg1	tests					906:910	the standard-prescribed biochemical tests	870:910	the standard-prescribed biochemical tests	870:910	They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum.
36367514	4	31	theme	metallic	538:545	arg1	sheen					547:551	a metallic sheen	536:551	a metallic sheen	536:551	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	10	32	theme	new	1484:1486	arg1	strains					1488:1494	the new strains	1480:1494	the new strains	1480:1494	The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis.
36367514	4	33	theme	moist	507:511	arg1	colonies					522:529	small, grey, moist and flat colonies	494:529	small, grey, moist and flat colonies	494:529	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	11	34	theme	Helicobacter	1692:1703	arg1	sp					1714:1715	the name Helicobacter colisuis sp	1683:1715	the name Helicobacter colisuis sp	1683:1715	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	9	35	from	species	1410:1416	arg1	distinct					1358:1365	distinct	1358:1365	distinct	1358:1365	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	2	36	theme	caecal	156:161	arg1	contents					163:170	caecal contents	156:170	caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	156:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	9	37	dep	related	1331:1337	arg1	strains					1310:1316	the three studied strains	1292:1316	the three studied strains	1292:1316	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	37	dep	related	1331:1337	arg1	related					1331:1337	related	1331:1337	related	1331:1337	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	37	dep	related	1331:1337	arg1	%					1350:1350	ANI ≥98.9 %	1340:1350	ANI ≥98.9 %	1340:1350	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	10	38	theme	distinct	1503:1510	arg1	clade					1512:1516	a distinct clade	1501:1516	a distinct clade most closely related to H. canadensis	1501:1554	The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis.
36367514	2	39	theme	pigs	188:191	arg1	contents					163:170	caecal contents	156:170	caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	156:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	11	40	theme	taxonomic	1582:1590	arg1	analysis					1592:1599	The conducted polyphasic taxonomic analysis	1557:1599	The conducted polyphasic taxonomic analysis	1557:1599	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	8	41	theme	gene	1139:1142	arg1	sequences					1144:1152	16S rRNA gene sequences	1130:1152	16S rRNA gene sequences	1130:1152	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	12	42	theme	CCUG	1776:1779	arg1	76053T					1781:1786	= DSM 113688T = CCUG 76053T	1760:1786	= DSM 113688T = CCUG 76053T	1760:1786	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	12	42	theme	CCUG	1776:1779	arg1	11154-15T					1749:1757	strain 11154-15T	1742:1757	strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain	1742:1806	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	10	43	theme	H.	1542:1543	arg1	canadensis					1545:1554	H. canadensis	1542:1554	H. canadensis	1542:1554	The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis.
36367514	9	44	theme	Helicobacter	1397:1408	arg1	species					1410:1416	the previously described Helicobacter species	1372:1416	the previously described Helicobacter species (ANI ≤90.6 %)	1372:1430	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	44	theme	Helicobacter	1397:1408	arg1	%					1429:1429	ANI ≤90.6 %	1419:1429	ANI ≤90.6 %	1419:1429	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	4	45	theme	modified	592:599	arg1	mCCDA					642:646	modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates	592:670	mCCDA	642:646	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	10	46	theme	related	1531:1537	arg1	clade					1512:1516	a distinct clade	1501:1516	a distinct clade most closely related to H. canadensis	1501:1554	The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis.
36367514	11	47	theme	Helicobacter	1652:1663	arg1	species					1665:1671	a novel Helicobacter species	1644:1671	a novel Helicobacter species for which the name Helicobacter colisuis sp	1644:1715	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	8	48	theme	high	1165:1168	arg1	similarity					1170:1179	a high similarity	1163:1179	a high similarity (≥99.8 % identity) to Helicobacter canadensis	1163:1225	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	8	48	theme	high	1165:1168	arg1	identity					1190:1197	≥99.8 % identity	1182:1197	≥99.8 % identity	1182:1197	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	2	49	from	sp	324:325	arg1	part					228:231	part	228:231	part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	228:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	9	50	theme	Pairwise	1228:1235	arg1	ANI					1266:1268	ANI	1266:1268	ANI	1266:1268	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	50	theme	Pairwise	1228:1235	arg1	identity					1256:1263	Pairwise average nucleotide identity	1228:1263	Pairwise average nucleotide identity (ANI) values	1228:1276	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	2	51	theme	monitoring	268:277	arg1	part					228:231	part	228:231	part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	228:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	12	52	theme	113688T	1766:1772	arg1	76053T					1781:1786	= DSM 113688T = CCUG 76053T	1760:1786	= DSM 113688T = CCUG 76053T	1760:1786	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	12	52	theme	113688T	1766:1772	arg1	11154-15T					1749:1757	strain 11154-15T	1742:1757	strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain	1742:1806	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	4	53	theme	Campylobacter-like	560:577	arg1	colonies					579:586	small Campylobacter-like colonies	554:586	small Campylobacter-like colonies	554:586	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	2	54	theme	EFSA-coordinated	240:255	arg1	monitoring					268:277	the EFSA-coordinated harmonized monitoring	236:277	the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	236:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	8	55	theme	%	1188:1188	arg1	similarity					1170:1179	a high similarity	1163:1179	a high similarity (≥99.8 % identity) to Helicobacter canadensis	1163:1225	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	8	55	theme	%	1188:1188	arg1	identity					1190:1197	≥99.8 % identity	1182:1197	≥99.8 % identity	1182:1197	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	2	56	theme	Helicobacter-like	110:126	arg1	isolates					128:135	Seven Helicobacter-like isolates	104:135	Seven Helicobacter-like isolates	104:135	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	11	57	theme	conducted	1561:1569	arg1	analysis					1592:1599	The conducted polyphasic taxonomic analysis	1557:1599	The conducted polyphasic taxonomic analysis	1557:1599	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	8	58	theme	16S	1130:1132	arg1	sequences					1144:1152	16S rRNA gene sequences	1130:1152	16S rRNA gene sequences	1130:1152	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	4	59	theme	agar	660:663	arg1	plates					665:670	modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates	592:670	plates	665:670	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	5	60	theme	bacterial	694:702	arg1	cells					704:708	the bacterial cells	690:708	the bacterial cells	690:708	Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1-2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive.
36367514	5	60	theme	bacterial	694:702	arg1	wide					774:777	wide	774:777	wide	774:777	Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1-2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive.
36367514	5	60	theme	bacterial	694:702	arg1	spirilli-shaped					715:729	spirilli-shaped	715:729	spirilli-shaped	715:729	Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1-2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive.
36367514	9	61	theme	nucleotide	1245:1254	arg1	ANI					1266:1268	ANI	1266:1268	ANI	1266:1268	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	61	theme	nucleotide	1245:1254	arg1	identity					1256:1263	Pairwise average nucleotide identity	1228:1263	Pairwise average nucleotide identity (ANI) values	1228:1276	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	2	62	theme	antimicrobial	282:294	arg1	resistance					296:305	antimicrobial resistance	282:305	antimicrobial resistance	282:305	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	8	63	theme	Helicobacter	1203:1214	arg1	canadensis					1216:1225	Helicobacter canadensis	1203:1225	Helicobacter canadensis	1203:1225	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	4	64	theme	Campylobacter	439:451	arg1	isolation					411:419	the isolation	407:419	the isolation of thermotolerant Campylobacter	407:451	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	4	65	theme	Agar	636:639	arg1	mCCDA					642:646	modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates	592:670	mCCDA	642:646	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	4	66	theme	extended	458:465	arg1	time					478:481	extended incubation time	458:481	extended incubation time	458:481	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	4	67	theme	flat	517:520	arg1	colonies					522:529	small, grey, moist and flat colonies	494:529	small, grey, moist and flat colonies	494:529	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	6	68	theme	MALDI-TOF	953:961	arg1	spectra					968:974	uniform, unique and reproducible MALDI-TOF mass spectra	920:974	uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum	920:1031	They could not be identified using the standard-prescribed biochemical tests and had uniform, unique and reproducible MALDI-TOF mass spectra that most closely matched those of Helicobacter pullorum.
36367514	5	69	dep	spirilli-shaped	715:729	arg1	cells					704:708	the bacterial cells	690:708	the bacterial cells	690:708	Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1-2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive.
36367514	5	69	dep	spirilli-shaped	715:729	arg1	wide					774:777	wide	774:777	wide	774:777	Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1-2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive.
36367514	5	69	dep	spirilli-shaped	715:729	arg1	spirilli-shaped					715:729	spirilli-shaped	715:729	spirilli-shaped	715:729	Morphologically, the bacterial cells were spirilli-shaped and highly motile, 1-2 µm long and ≤0.5 µm wide, Gram-negative, oxidase-positive and catalase-positive.
36367514	9	70	theme	studied	1302:1308	arg1	strains					1310:1316	the three studied strains	1292:1316	the three studied strains	1292:1316	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	70	theme	studied	1302:1308	arg1	related					1331:1337	related	1331:1337	related	1331:1337	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	70	theme	studied	1302:1308	arg1	%					1350:1350	ANI ≥98.9 %	1340:1350	ANI ≥98.9 %	1340:1350	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	1	71	theme	caecal	46:51	arg1	contents					53:60	caecal contents	46:60	caecal contents of domestic pigs (Sus scrofa domesticus)	46:101	nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus).
36367514	2	72	dep	Sus	194:196	arg1	domesticus					205:214	domesticus	205:214	Sus scrofa domesticus	194:214	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	7	73	dep	strains	1040:1046	arg1	14348-15					1060:1067	14348-15	1060:1067	14348-15	1060:1067	Three strains (11154-15T, 14348-15 and 16470-15) underwent whole-genome sequencing.
36367514	7	73	dep	strains	1040:1046	arg1	11154-15T					1049:1057	11154-15T	1049:1057	11154-15T	1049:1057	Three strains (11154-15T, 14348-15 and 16470-15) underwent whole-genome sequencing.
36367514	7	73	dep	strains	1040:1046	arg1	16470-15					1073:1080	16470-15	1073:1080	16470-15	1073:1080	Three strains (11154-15T, 14348-15 and 16470-15) underwent whole-genome sequencing.
36367514	0	74	theme	colisuis	13:20	arg1	sp					22:23	Helicobacter colisuis sp	0:23	Helicobacter colisuis sp.	0:24	Helicobacter colisuis sp.
36367514	9	75	theme	ANI	1340:1342	arg1	strains					1310:1316	the three studied strains	1292:1316	the three studied strains	1292:1316	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	75	theme	ANI	1340:1342	arg1	related					1331:1337	related	1331:1337	related	1331:1337	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	75	theme	ANI	1340:1342	arg1	%					1350:1350	ANI ≥98.9 %	1340:1350	ANI ≥98.9 %	1340:1350	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	1	76	theme	pigs	74:77	arg1	contents					53:60	caecal contents	46:60	caecal contents of domestic pigs (Sus scrofa domesticus)	46:101	nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus).
36367514	10	77	theme	genome-based	1442:1453	arg1	phylogeny					1455:1463	The core genome-based phylogeny	1433:1463	The core genome-based phylogeny	1433:1463	The core genome-based phylogeny confirmed that the new strains form a distinct clade most closely related to H. canadensis.
36367514	11	78	theme	colisuis	1705:1712	arg1	sp					1714:1715	the name Helicobacter colisuis sp	1683:1715	the name Helicobacter colisuis sp	1683:1715	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	2	79	from	part	228:231	arg1	sp					324:325	Campylobacter sp	310:325	Campylobacter sp	310:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	11	80	theme	name	1687:1690	arg1	sp					1714:1715	the name Helicobacter colisuis sp	1683:1715	the name Helicobacter colisuis sp	1683:1715	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	8	81	theme	rRNA	1134:1137	arg1	sequences					1144:1152	16S rRNA gene sequences	1130:1152	16S rRNA gene sequences	1130:1152	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	12	82	theme	=	1760:1760	arg1	76053T					1781:1786	= DSM 113688T = CCUG 76053T	1760:1786	= DSM 113688T = CCUG 76053T	1760:1786	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	12	82	theme	=	1760:1760	arg1	11154-15T					1749:1757	strain 11154-15T	1742:1757	strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain	1742:1806	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	11	83	theme	polyphasic	1571:1580	arg1	analysis					1592:1599	The conducted polyphasic taxonomic analysis	1557:1599	The conducted polyphasic taxonomic analysis	1557:1599	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	8	84	theme	sequences	1144:1152	arg1	Analysis					1118:1125	Analysis	1118:1125	Analysis of 16S rRNA gene sequences	1118:1152	Analysis of 16S rRNA gene sequences revealed a high similarity (≥99.8 % identity) to Helicobacter canadensis.
36367514	12	85	theme	type	1796:1799	arg1	strain					1801:1806	the type strain	1792:1806	the type strain	1792:1806	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	2	86	theme	domestic	179:186	arg1	pigs					188:191	100 domestic pigs	175:191	100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp	175:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	2	87	theme	Campylobacter	310:322	arg1	sp					324:325	Campylobacter sp	310:325	Campylobacter sp	310:325	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
36367514	9	88	theme	described	1387:1395	arg1	species					1410:1416	the previously described Helicobacter species	1372:1416	the previously described Helicobacter species (ANI ≤90.6 %)	1372:1430	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	9	88	theme	described	1387:1395	arg1	%					1429:1429	ANI ≤90.6 %	1419:1429	ANI ≤90.6 %	1419:1429	Pairwise average nucleotide identity (ANI) values revealed that the three studied strains were closely related (ANI ≥98.9 %), but distinct from the previously described Helicobacter species (ANI ≤90.6 %).
36367514	12	89	theme	=	1774:1774	arg1	76053T					1781:1786	= DSM 113688T = CCUG 76053T	1760:1786	= DSM 113688T = CCUG 76053T	1760:1786	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	12	89	theme	=	1774:1774	arg1	11154-15T					1749:1757	strain 11154-15T	1742:1757	strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain	1742:1806	nov. is suggested, with strain 11154-15T (= DSM 113688T = CCUG 76053T) as the type strain.
36367514	4	90	theme	Charcoal-Cefoperazone-Deoxycholate	601:634	arg1	mCCDA					642:646	modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates	592:670	mCCDA	642:646	The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small Campylobacter-like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
36367514	11	91	theme	novel	1646:1650	arg1	species					1665:1671	a novel Helicobacter species	1644:1671	a novel Helicobacter species for which the name Helicobacter colisuis sp	1644:1715	The conducted polyphasic taxonomic analysis confirmed that the three strains represent a novel Helicobacter species for which the name Helicobacter colisuis sp.
36367514	2	92	dep	pigs	188:191	arg1	Sus					194:196	Sus	194:196	Sus	194:196	Seven Helicobacter-like isolates were cultured from caecal contents of 100 domestic pigs (Sus scrofa domesticus) sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in Campylobacter sp.
35934080	3	0	theme	cellulose	388:396	arg1	functionalization					398:414	cellulose functionalization	388:414	cellulose functionalization	388:414	Here, we aim to utilize inexpensive fertilizer-grade phosphate for cellulose functionalization.
35934080	4	1	theme	hydrogen	571:578	arg1	phosphate					580:588	a fertilizer-grade diammonium hydrogen phosphate	541:588	a fertilizer-grade diammonium hydrogen phosphate (DAP)	541:594	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	1	theme	hydrogen	571:578	arg1	DAP					591:593	DAP	591:593	DAP	591:593	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	5	2	theme	phosphorylated	941:954	arg1	materials					956:964	the phosphorylated materials	937:964	the phosphorylated materials	937:964	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	6	3	theme	charge	1061:1066	arg1	content					1068:1074	the same charge content	1052:1074	the same charge content as that registered when using the reagent-grade DAP	1052:1126	It was found that cellulose phosphorylation using DAP fertilizer gave materials with the same charge content as that registered when using the reagent-grade DAP.
35934080	0	4	theme	paper	98:102	arg1	sorbent					104:110	excellent methylene blue paper sorbent	73:110	excellent methylene blue paper sorbent	73:110	Powerful cellulose phosphorylation by fertilizer-grade phosphate enables excellent methylene blue paper sorbent.
35934080	11	5	theme	second	2079:2084	arg1	cycle					2086:2090	the second cycle	2075:2090	the second cycle	2075:2090	Adsorption/desorption tests confirmed the reusability and regeneration of the paper with a recovery of 100 % for MB in the second cycle.
35934080	1	6	theme	various	206:212	arg1	applications					214:225	various applications	206:225	various applications	206:225	Cellulose is an interesting biopolymer offering numerous functionalization possibilities for various applications.
35934080	8	7	theme	corresponding	1290:1302	arg1	sorbent					1371:1377	sorbent	1371:1377	sorbent	1371:1377	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	8	7	theme	corresponding	1290:1302	arg1	P-CMF					1324:1328	P-CMF	1324:1328	P-CMF	1324:1328	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	8	7	theme	corresponding	1290:1302	arg1	CMF					1319:1321	corresponding phosphorylated CMF	1290:1321	The corresponding phosphorylated CMF (P-CMF)	1286:1329	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	4	8	theme	water-based	632:642	arg1	protocol					644:651	a water-based protocol	630:651	a water-based protocol	630:651	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	10	9	theme	~1200 mg·g-1	1763:1774	arg1	kinetics					1676:1683	fast adsorption kinetics	1660:1683	fast adsorption kinetics (2-6 h)	1660:1691	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	9	theme	~1200 mg·g-1	1763:1774	arg1	capacity					1751:1758	a MB adsorption capacity	1735:1758	a MB adsorption capacity of ~1200 mg·g-1	1735:1774	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	9	theme	~1200 mg·g-1	1763:1774	arg1	%					1728:1728	92-99 %	1722:1728	92-99 %	1722:1728	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	9	theme	~1200 mg·g-1	1763:1774	arg1	efficiency					1710:1719	high adsorption efficiency	1694:1719	high adsorption efficiency (92-99 %)	1694:1729	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	9	theme	~1200 mg·g-1	1763:1774	arg1	2-6 h					1686:1690	2-6 h	1686:1690	2-6 h	1686:1690	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	11	10	theme	paper	2034:2038	arg1	regeneration					2014:2025	regeneration	2014:2025	regeneration	2014:2025	Adsorption/desorption tests confirmed the reusability and regeneration of the paper with a recovery of 100 % for MB in the second cycle.
35934080	11	10	theme	paper	2034:2038	arg1	reusability					1998:2008	reusability	1998:2008	reusability	1998:2008	Adsorption/desorption tests confirmed the reusability and regeneration of the paper with a recovery of 100 % for MB in the second cycle.
35934080	8	11	theme	aqueous	1416:1422	arg1	solutions					1424:1432	aqueous solutions	1416:1432	aqueous solutions with different concentrations	1416:1462	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	5	12	theme	conductometric	726:739	arg1	titration					741:749	conductometric titration	726:749	conductometric titration	726:749	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	5	13	theme	DAP	668:670	arg1	effect					658:663	The effect	654:663	The effect of DAP on the phosphorylation reaction	654:702	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	5	14	from	effect	658:663	arg1	reaction					695:702	the phosphorylation reaction	675:702	the phosphorylation reaction	675:702	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	10	15	theme	MB	1737:1738	arg1	capacity					1751:1758	a MB adsorption capacity	1735:1758	a MB adsorption capacity of ~1200 mg·g-1	1735:1774	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	7	16	theme	charge	1254:1259	arg1	7000 mmol·g-1					1270:1282	7000 mmol·g-1	1270:1282	7000 mmol·g-1	1270:1282	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	7	16	theme	charge	1254:1259	arg1	content					1261:1267	high charge content	1249:1267	high charge content (7000 mmol·g-1)	1249:1283	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	8	17	with	solutions	1424:1432	arg1	concentrations					1449:1462	different concentrations	1439:1462	different concentrations	1439:1462	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	6	18	with	materials	1037:1045	arg1	content					1068:1074	the same charge content	1052:1074	the same charge content as that registered when using the reagent-grade DAP	1052:1126	It was found that cellulose phosphorylation using DAP fertilizer gave materials with the same charge content as that registered when using the reagent-grade DAP.
35934080	8	19	theme	methylene	1383:1391	arg1	removal					1403:1409	methylene blue (MB) removal	1383:1409	methylene blue (MB) removal from aqueous solutions with different concentrations	1383:1462	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	7	20	theme	DAP	1211:1213	arg1	amount					1184:1189	the amount	1180:1189	the amount of fertilizer-grade DAP used for the phosphorylation	1180:1242	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	7	20	theme	DAP	1211:1213	arg1	DAP					1211:1213	fertilizer-grade DAP	1194:1213	fertilizer-grade DAP	1194:1213	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	4	21	theme	diammonium	560:569	arg1	phosphate					580:588	a fertilizer-grade diammonium hydrogen phosphate	541:588	a fertilizer-grade diammonium hydrogen phosphate (DAP)	541:594	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	21	theme	diammonium	560:569	arg1	DAP					591:593	DAP	591:593	DAP	591:593	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	5	22	theme	phosphorylation	679:693	arg1	reaction					695:702	the phosphorylation reaction	675:702	the phosphorylation reaction	675:702	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	7	23	theme	high	1249:1252	arg1	7000 mmol·g-1					1270:1282	7000 mmol·g-1	1270:1282	7000 mmol·g-1	1270:1282	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	7	23	theme	high	1249:1252	arg1	content					1261:1267	high charge content	1249:1267	high charge content (7000 mmol·g-1)	1249:1283	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	8	24	theme	phosphorylated	1304:1317	arg1	sorbent					1371:1377	sorbent	1371:1377	sorbent	1371:1377	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	8	24	theme	phosphorylated	1304:1317	arg1	P-CMF					1324:1328	P-CMF	1324:1328	P-CMF	1324:1328	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	8	24	theme	phosphorylated	1304:1317	arg1	CMF					1319:1321	corresponding phosphorylated CMF	1290:1321	The corresponding phosphorylated CMF (P-CMF)	1286:1329	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	0	25	theme	cellulose	9:17	arg1	phosphorylation					19:33	Powerful cellulose phosphorylation	0:33	Powerful cellulose phosphorylation by fertilizer-grade phosphate	0:63	Powerful cellulose phosphorylation by fertilizer-grade phosphate enables excellent methylene blue paper sorbent.
35934080	4	26	theme	fertilizer-grade	543:558	arg1	phosphate					580:588	a fertilizer-grade diammonium hydrogen phosphate	541:588	a fertilizer-grade diammonium hydrogen phosphate (DAP)	541:594	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	26	theme	fertilizer-grade	543:558	arg1	DAP					591:593	DAP	591:593	DAP	591:593	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	7	27	theme	reaction	1144:1151	arg1	conditions					1153:1162	the reaction conditions	1140:1162	the reaction conditions	1140:1162	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	11	28	from	recovery	2047:2054	arg1	cycle					2086:2090	the second cycle	2075:2090	the second cycle	2075:2090	Adsorption/desorption tests confirmed the reusability and regeneration of the paper with a recovery of 100 % for MB in the second cycle.
35934080	9	29	theme	adsorption	1633:1642	arg1	isotherms					1644:1652	the adsorption isotherms	1629:1652	the adsorption isotherms	1629:1652	The findings indicated that the pseudo-second-order model could be useful to assess the adsorption kinetics while the Langmuir isotherm model can suitably describe the adsorption isotherms.
35934080	5	30	theme	thermal	914:920	arg1	degradation					922:932	thermal degradation	914:932	thermal degradation of the phosphorylated materials	914:964	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	0	31	theme	Powerful	0:7	arg1	phosphorylation					19:33	Powerful cellulose phosphorylation	0:33	Powerful cellulose phosphorylation by fertilizer-grade phosphate	0:63	Powerful cellulose phosphorylation by fertilizer-grade phosphate enables excellent methylene blue paper sorbent.
35934080	4	32	theme	urea	615:618	arg1	presence					603:610	the presence	599:610	the presence of urea following a water-based protocol	599:651	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	10	33	theme	wastewater	1934:1943	arg1	effluents					1945:1953	dye-contaminated wastewater effluents	1917:1953	dye-contaminated wastewater effluents	1917:1953	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	4	34	theme	Giant	464:468	arg1	GR					476:477	GR	476:477	GR	476:477	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	34	theme	Giant	464:468	arg1	Reed					470:473	Giant Reed	464:473	Giant Reed (GR)	464:478	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	10	35	theme	adsorption	1699:1708	arg1	efficiency					1710:1719	high adsorption efficiency	1694:1719	high adsorption efficiency (92-99 %)	1694:1729	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	35	theme	adsorption	1699:1708	arg1	%					1728:1728	92-99 %	1722:1728	92-99 %	1722:1728	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	36	theme	adsorption	1665:1674	arg1	kinetics					1676:1683	fast adsorption kinetics	1660:1683	fast adsorption kinetics (2-6 h)	1660:1691	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	36	theme	adsorption	1665:1674	arg1	2-6 h					1686:1690	2-6 h	1686:1690	2-6 h	1686:1690	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	11	37	dep	reusability	1998:2008	arg1	the					1994:1996	the	1994:1996	the	1994:1996	Adsorption/desorption tests confirmed the reusability and regeneration of the paper with a recovery of 100 % for MB in the second cycle.
35934080	10	38	theme	cellulose-based	1821:1835	arg1	sorbents					1837:1844	cellulose-based sorbents	1821:1844	cellulose-based sorbents	1821:1844	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	8	39	theme	different	1439:1447	arg1	concentrations					1449:1462	different concentrations	1439:1462	different concentrations	1439:1462	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	5	40	theme	charge	883:888	arg1	content					890:896	charge content	883:896	charge content	883:896	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	10	41	theme	fast	1660:1663	arg1	kinetics					1676:1683	fast adsorption kinetics	1660:1683	fast adsorption kinetics (2-6 h)	1660:1691	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	41	theme	fast	1660:1663	arg1	2-6 h					1686:1690	2-6 h	1686:1690	2-6 h	1686:1690	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	42	theme	dye-contaminated	1917:1932	arg1	effluents					1945:1953	dye-contaminated wastewater effluents	1917:1953	dye-contaminated wastewater effluents	1917:1953	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	5	43	theme	materials	956:964	arg1	structure					899:907	structure	899:907	structure	899:907	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	5	43	theme	materials	956:964	arg1	composition					870:880	composition	870:880	composition	870:880	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	5	43	theme	materials	956:964	arg1	content					890:896	charge content	883:896	charge content	883:896	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	5	43	theme	materials	956:964	arg1	morphology					858:867	the morphology	854:867	the morphology	854:867	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	5	43	theme	materials	956:964	arg1	degradation					922:932	thermal degradation	914:932	thermal degradation of the phosphorylated materials	914:964	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	10	44	theme	high	1694:1697	arg1	efficiency					1710:1719	high adsorption efficiency	1694:1719	high adsorption efficiency (92-99 %)	1694:1729	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	44	theme	high	1694:1697	arg1	%					1728:1728	92-99 %	1722:1728	92-99 %	1722:1728	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	6	45	theme	DAP	1017:1019	arg1	fertilizer					1021:1030	DAP fertilizer	1017:1030	DAP fertilizer	1017:1030	It was found that cellulose phosphorylation using DAP fertilizer gave materials with the same charge content as that registered when using the reagent-grade DAP.
35934080	2	46	theme	complex	302:308	arg1	processes					310:318	complex processes	302:318	complex processes	302:318	Yet, cellulose functionalization usually involves expensive chemicals and complex processes.
35934080	11	47	theme	Adsorption/desorption	1956:1976	arg1	tests					1978:1982	Adsorption/desorption tests	1956:1982	Adsorption/desorption tests	1956:1982	Adsorption/desorption tests confirmed the reusability and regeneration of the paper with a recovery of 100 % for MB in the second cycle.
35934080	9	48	theme	isotherm	1592:1599	arg1	model					1601:1605	the Langmuir isotherm model	1579:1605	the Langmuir isotherm model	1579:1605	The findings indicated that the pseudo-second-order model could be useful to assess the adsorption kinetics while the Langmuir isotherm model can suitably describe the adsorption isotherms.
35934080	4	49	attach	isolated	450:457	arg2	CMF					440:442	CMF	440:442	CMF	440:442	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	49	attach	isolated	450:457	arg1	GR					476:477	GR	476:477	GR	476:477	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	49	attach	isolated	450:457	arg2	microfibers					427:437	Cellulose microfibers	417:437	Cellulose microfibers (CMF)	417:443	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	49	attach	isolated	450:457	arg1	Reed					470:473	Giant Reed	464:473	Giant Reed (GR)	464:478	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	0	50	theme	fertilizer-grade	38:53	arg1	phosphate					55:63	fertilizer-grade phosphate	38:63	fertilizer-grade phosphate	38:63	Powerful cellulose phosphorylation by fertilizer-grade phosphate enables excellent methylene blue paper sorbent.
35934080	6	51	theme	same	1056:1059	arg1	content					1068:1074	the same charge content	1052:1074	the same charge content as that registered when using the reagent-grade DAP	1052:1126	It was found that cellulose phosphorylation using DAP fertilizer gave materials with the same charge content as that registered when using the reagent-grade DAP.
35934080	8	52	used	used	1363:1366	arg2	sorbent					1371:1377	sorbent	1371:1377	sorbent	1371:1377	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	8	52	used	used	1363:1366	arg2	P-CMF					1324:1328	P-CMF	1324:1328	P-CMF	1324:1328	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	8	52	used	used	1363:1366	arg2	CMF					1319:1321	corresponding phosphorylated CMF	1290:1321	The corresponding phosphorylated CMF (P-CMF)	1286:1329	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	0	53	theme	excellent	73:81	arg1	sorbent					104:110	excellent methylene blue paper sorbent	73:110	excellent methylene blue paper sorbent	73:110	Powerful cellulose phosphorylation by fertilizer-grade phosphate enables excellent methylene blue paper sorbent.
35934080	10	54	theme	effective	1892:1900	arg1	remediation					1902:1912	the effective remediation	1888:1912	the effective remediation of dye-contaminated wastewater effluents	1888:1953	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	55	theme	P-CMF	1851:1855	arg1	paper					1857:1861	the P-CMF paper	1847:1861	the P-CMF paper	1847:1861	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	1	56	theme	interesting	129:139	arg1	Cellulose					113:121	Cellulose	113:121	Cellulose	113:121	Cellulose is an interesting biopolymer offering numerous functionalization possibilities for various applications.
35934080	1	56	theme	interesting	129:139	arg1	biopolymer					141:150	an interesting biopolymer	126:150	an interesting biopolymer offering numerous functionalization possibilities for various applications	126:225	Cellulose is an interesting biopolymer offering numerous functionalization possibilities for various applications.
35934080	5	57	theme	further	776:782	arg1	characterization					784:799	further characterization	776:799	further characterization	776:799	The effect of DAP on the phosphorylation reaction was mainly studied by conductometric titration, ICP-OES and FTIR, while further characterization was performed by SEM/EDX, TGA and XRD to investigate the morphology, composition, charge content, structure, and thermal degradation of the phosphorylated materials.
35934080	6	58	theme	cellulose	985:993	arg1	phosphorylation					995:1009	cellulose phosphorylation	985:1009	cellulose phosphorylation using DAP fertilizer	985:1030	It was found that cellulose phosphorylation using DAP fertilizer gave materials with the same charge content as that registered when using the reagent-grade DAP.
35934080	2	59	theme	expensive	278:286	arg1	chemicals					288:296	expensive chemicals	278:296	expensive chemicals	278:296	Yet, cellulose functionalization usually involves expensive chemicals and complex processes.
35934080	9	60	theme	adsorption	1553:1562	arg1	kinetics					1564:1571	the adsorption kinetics	1549:1571	the adsorption kinetics	1549:1571	The findings indicated that the pseudo-second-order model could be useful to assess the adsorption kinetics while the Langmuir isotherm model can suitably describe the adsorption isotherms.
35934080	3	61	theme	inexpensive	345:355	arg1	phosphate					374:382	inexpensive fertilizer-grade phosphate	345:382	inexpensive fertilizer-grade phosphate	345:382	Here, we aim to utilize inexpensive fertilizer-grade phosphate for cellulose functionalization.
35934080	11	62	theme	%	2063:2063	arg1	recovery					2047:2054	a recovery	2045:2054	a recovery of 100 % for MB in the second cycle	2045:2090	Adsorption/desorption tests confirmed the reusability and regeneration of the paper with a recovery of 100 % for MB in the second cycle.
35934080	10	63	theme	adsorption	1740:1749	arg1	capacity					1751:1758	a MB adsorption capacity	1735:1758	a MB adsorption capacity of ~1200 mg·g-1	1735:1774	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	3	64	theme	fertilizer-grade	357:372	arg1	phosphate					374:382	inexpensive fertilizer-grade phosphate	345:382	inexpensive fertilizer-grade phosphate	345:382	Here, we aim to utilize inexpensive fertilizer-grade phosphate for cellulose functionalization.
35934080	10	65	theme	effluents	1945:1953	arg1	remediation					1902:1912	the effective remediation	1888:1912	the effective remediation of dye-contaminated wastewater effluents	1888:1953	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	10	66	dep	great	1869:1873	arg1	promises					1875:1882	promises	1875:1882	promises	1875:1882	With fast adsorption kinetics (2-6 h), high adsorption efficiency (92-99 %) and a MB adsorption capacity of ~1200 mg·g-1 surpassing what has been reported so far for cellulose-based sorbents, the P-CMF paper holds great promises for the effective remediation of dye-contaminated wastewater effluents.
35934080	1	67	theme	numerous	161:168	arg1	possibilities					188:200	numerous functionalization possibilities	161:200	numerous functionalization possibilities for various applications	161:225	Cellulose is an interesting biopolymer offering numerous functionalization possibilities for various applications.
35934080	8	68	theme	blue	1393:1396	arg1	MB					1399:1400	MB	1399:1400	MB	1399:1400	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	8	68	theme	blue	1393:1396	arg1	methylene					1383:1391	methylene blue	1383:1396	methylene blue (MB) removal from aqueous solutions with different concentrations	1383:1462	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
35934080	0	69	theme	blue	93:96	arg1	sorbent					104:110	excellent methylene blue paper sorbent	73:110	excellent methylene blue paper sorbent	73:110	Powerful cellulose phosphorylation by fertilizer-grade phosphate enables excellent methylene blue paper sorbent.
35934080	2	70	theme	cellulose	233:241	arg1	functionalization					243:259	cellulose functionalization	233:259	cellulose functionalization	233:259	Yet, cellulose functionalization usually involves expensive chemicals and complex processes.
35934080	6	71	theme	reagent-grade	1110:1122	arg1	DAP					1124:1126	the reagent-grade DAP	1106:1126	the reagent-grade DAP	1106:1126	It was found that cellulose phosphorylation using DAP fertilizer gave materials with the same charge content as that registered when using the reagent-grade DAP.
35934080	1	72	theme	functionalization	170:186	arg1	possibilities					188:200	numerous functionalization possibilities	161:200	numerous functionalization possibilities for various applications	161:225	Cellulose is an interesting biopolymer offering numerous functionalization possibilities for various applications.
35934080	9	73	theme	pseudo-second-order	1497:1515	arg1	model					1517:1521	the pseudo-second-order model	1493:1521	the pseudo-second-order model	1493:1521	The findings indicated that the pseudo-second-order model could be useful to assess the adsorption kinetics while the Langmuir isotherm model can suitably describe the adsorption isotherms.
35934080	9	73	theme	pseudo-second-order	1497:1515	arg1	useful					1532:1537	useful	1532:1537	useful	1532:1537	The findings indicated that the pseudo-second-order model could be useful to assess the adsorption kinetics while the Langmuir isotherm model can suitably describe the adsorption isotherms.
35934080	7	74	theme	fertilizer-grade	1194:1209	arg1	DAP					1211:1213	fertilizer-grade DAP	1194:1213	fertilizer-grade DAP	1194:1213	Optimizing the reaction conditions with respect to the amount of fertilizer-grade DAP used for the phosphorylation gave high charge content (7000 mmol·g-1).
35934080	4	75	theme	Cellulose	417:425	arg1	CMF					440:442	CMF	440:442	CMF	440:442	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	4	75	theme	Cellulose	417:425	arg1	microfibers					427:437	Cellulose microfibers	417:437	Cellulose microfibers (CMF)	417:443	Cellulose microfibers (CMF) were isolated from Giant Reed (GR) and were then phosphorylated using either a reagent-grade or a fertilizer-grade diammonium hydrogen phosphate (DAP) in the presence of urea following a water-based protocol.
35934080	9	76	theme	Langmuir	1583:1590	arg1	model					1601:1605	the Langmuir isotherm model	1579:1605	the Langmuir isotherm model	1579:1605	The findings indicated that the pseudo-second-order model could be useful to assess the adsorption kinetics while the Langmuir isotherm model can suitably describe the adsorption isotherms.
35934080	8	77	from	solutions	1424:1432	arg1	removal					1403:1409	methylene blue (MB) removal	1383:1409	methylene blue (MB) removal from aqueous solutions with different concentrations	1383:1462	The corresponding phosphorylated CMF (P-CMF) were processed into a paper and used as sorbent for methylene blue (MB) removal from aqueous solutions with different concentrations.
37099324	9	0	from	impact	1427:1432	arg1	survival					1458:1465	stem/progenitor cell survival	1437:1465	stem/progenitor cell survival (>95%)	1437:1472	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	9	0	from	impact	1427:1432	arg1	%					1471:1471	>95%	1468:1471	>95%	1468:1471	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	9	0	from	impact	1427:1432	arg1	differentiation					1477:1491	differentiation	1477:1491	differentiation (∼90%)	1477:1498	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	9	0	from	impact	1427:1432	arg1	%					1497:1497	∼90%	1494:1497	∼90%	1494:1497	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	7	1	theme	mature	1245:1250	arg1	marker					1261:1266	a mature neuronal marker	1243:1266	a mature neuronal marker (MAP-2, day 8)	1243:1281	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	1	theme	mature	1245:1250	arg1	MAP-2					1269:1273	MAP-2	1269:1273	MAP-2	1269:1273	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	2	theme	neurospheres	1097:1108	arg1	expression					1129:1138	stage-specific expression	1114:1138	stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%)	1114:1325	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	2	theme	neurospheres	1097:1108	arg1	generation					1073:1082	the generation	1069:1082	the generation of prominent neurospheres	1069:1108	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	11	3	theme	self-healing	1833:1844	arg1	properties					1846:1855	self-healing properties	1833:1855	self-healing properties mimicking native neural tissue	1833:1886	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	12	4	theme	stem/neural	1987:1997	arg1	growth					2015:2020	stem/neural progenitor cell growth	1987:2020	stem/neural progenitor cell growth	1987:2020	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	11	5	theme	native	1867:1872	arg1	tissue					1881:1886	native neural tissue	1867:1886	native neural tissue	1867:1886	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	2	6	theme	stem	286:289	arg1	ADMSC					298:302	ADMSC	298:302	ADMSC	298:302	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	2	6	theme	stem	286:289	arg1	cells					291:295	adipose-derived mesenchymal stem cells	258:295	adipose-derived mesenchymal stem cells (ADMSC)	258:303	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	12	7	theme	asiatic	1952:1958	arg1	acid					1960:1963	asiatic acid	1952:1963	asiatic acid	1952:1963	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	12	8	theme	transplantation	2136:2150	arg1	site					2152:2155	the cell transplantation site	2127:2155	the cell transplantation site	2127:2155	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	11	9	theme	porous	1774:1779	arg1	niche					1790:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	7	10	theme	progenitor	1152:1161	arg1	marker					1163:1168	a neural progenitor marker	1143:1168	a neural progenitor marker (nestin, day 4)	1143:1184	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	10	theme	progenitor	1152:1161	arg1	nestin					1171:1176	nestin	1171:1176	nestin	1171:1176	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	9	11	theme	stem/progenitor	1437:1451	arg1	survival					1458:1465	stem/progenitor cell survival	1437:1465	stem/progenitor cell survival (>95%)	1437:1472	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	9	11	theme	stem/progenitor	1437:1451	arg1	%					1471:1471	>95%	1468:1471	>95%	1468:1471	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	12	12	contain	have	2046:2049	arg2	antioxidants					2077:2088	antioxidants	2077:2088	antioxidants	2077:2088	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	12	12	contain	have	2046:2049	arg1	hydrogel					1906:1913	ADA-gelatin hydrogel	1894:1913	ADA-gelatin hydrogel by itself	1894:1923	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	12	12	contain	have	2046:2049	arg2	applications					2061:2072	potential applications	2051:2072	potential applications	2051:2072	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	12	12	contain	have	2046:2049	arg2	promoters					2101:2109	growth promoters	2094:2109	growth promoters	2094:2109	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	11	13	theme	Optimized	1749:1757	arg1	niche					1790:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	5	14	theme	hydrogel	728:735	arg1	system					737:742	an appropriate injectable hydrogel system	702:742	an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation	702:806	Here, we focus on identifying an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation for neural tissue engineering.
37099324	11	15	theme	neural	1874:1879	arg1	tissue					1881:1886	native neural tissue	1867:1886	native neural tissue	1867:1886	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	2	16	theme	neural	372:377	arg1	tissues					379:385	neural tissues	372:385	neural tissues	372:385	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	10	17	theme	asiatic	1583:1589	arg1	acid					1591:1594	asiatic acid	1583:1594	asiatic acid specific for neural niche	1583:1620	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	11	18	theme	rapid	1806:1810	arg1	min					1824:1826	3 min	1822:1826	3 min	1822:1826	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	11	18	theme	rapid	1806:1810	arg1	gelation					1812:1819	rapid gelation	1806:1819	rapid gelation (3 min)	1806:1827	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	0	19	theme	Scaffold	122:129	arg1	Role					79:82	The Role	75:82	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.	0:173	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	1	20	theme	limited	206:212	arg1	potential					227:235	limited regeneration potential	206:235	limited regeneration potential	206:235	The central nervous system has limited regeneration potential.
37099324	5	21	dep	cell	772:775	arg1	attachment					777:786	attachment	777:786	attachment	777:786	Here, we focus on identifying an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation for neural tissue engineering.
37099324	10	22	theme	quantities	1569:1578	arg1	Addition					1545:1552	Addition	1545:1552	Addition of appropriate quantities of asiatic acid specific for neural niche	1545:1620	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	13	23	theme	delivery	2283:2290	arg1	matrix					2172:2177	the matrix itself or incorporated with phytomoieties	2168:2219	the matrix itself or incorporated with phytomoieties	2168:2219	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	13	23	theme	delivery	2283:2290	arg1	vehicle					2292:2298	a potential minimally invasive injectable cell delivery vehicle	2236:2298	a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases	2236:2342	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	7	24	theme	prominent	1087:1095	arg1	neurospheres					1097:1108	prominent neurospheres	1087:1108	prominent neurospheres	1087:1108	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	2	25	theme	adipose-derived	258:272	arg1	ADMSC					298:302	ADMSC	298:302	ADMSC	298:302	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	2	25	theme	adipose-derived	258:272	arg1	cells					291:295	adipose-derived mesenchymal stem cells	258:295	adipose-derived mesenchymal stem cells (ADMSC)	258:303	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	5	26	theme	appropriate	705:715	arg1	system					737:742	an appropriate injectable hydrogel system	702:742	an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation	702:806	Here, we focus on identifying an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation for neural tissue engineering.
37099324	12	27	theme	potential	2051:2059	arg1	antioxidants					2077:2088	antioxidants	2077:2088	antioxidants	2077:2088	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	12	27	theme	potential	2051:2059	arg1	promoters					2101:2109	growth promoters	2094:2109	growth promoters	2094:2109	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	12	27	theme	potential	2051:2059	arg1	applications					2061:2072	potential applications	2051:2072	potential applications	2051:2072	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	5	28	theme	neural	812:817	arg1	engineering					826:836	neural tissue engineering	812:836	neural tissue engineering	812:836	Here, we focus on identifying an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation for neural tissue engineering.
37099324	13	29	theme	injectable	2267:2276	arg1	matrix					2172:2177	the matrix itself or incorporated with phytomoieties	2168:2219	the matrix itself or incorporated with phytomoieties	2168:2219	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	13	29	theme	injectable	2267:2276	arg1	vehicle					2292:2298	a potential minimally invasive injectable cell delivery vehicle	2236:2298	a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases	2236:2342	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	5	30	theme	stem/progenitor	756:770	arg1	cell					772:775	stem/progenitor cell attachment and differentiation	756:806	cell	772:775	Here, we focus on identifying an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation for neural tissue engineering.
37099324	0	31	dep	Injectable	90:99	arg1	Resorbable					102:111	Resorbable	102:111	Resorbable	102:111	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	7	32	with	generation	1073:1082	arg1	networking					1309:1318	networking	1309:1318	networking (>85%)	1309:1325	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	32	with	generation	1073:1082	arg1	%					1324:1324	>85%	1321:1324	>85%	1321:1324	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	32	with	generation	1073:1082	arg1	branching					1295:1303	neural branching	1288:1303	neural branching	1288:1303	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	0	33	theme	Injectable	90:99	arg1	Scaffold					122:129	an Injectable, Resorbable Hydrogel Scaffold	87:129	an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin	87:172	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	0	34	theme	Adipose-Derived	35:49	arg1	Cells					68:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	9	35	theme	two-dimensional	1515:1529	arg1	culture					1536:1542	two-dimensional (2D) culture	1515:1542	two-dimensional (2D) culture	1515:1542	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	2	36	theme	tissues	379:385	arg1	regeneration					356:367	the regeneration	352:367	the regeneration of neural tissues	352:385	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	0	37	theme	Neural	0:5	arg1	Engineering					14:24	Neural Tissue Engineering	0:24	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.	0:173	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	13	38	dep	potential	2238:2246	arg1	invasive					2258:2265	invasive	2258:2265	invasive	2258:2265	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	7	39	theme	marker	1212:1217	arg1	expression					1129:1138	stage-specific expression	1114:1138	stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%)	1114:1325	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	39	theme	marker	1212:1217	arg1	generation					1073:1082	the generation	1069:1082	the generation of prominent neurospheres	1069:1108	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	40	with	expression	1129:1138	arg1	networking					1309:1318	networking	1309:1318	networking (>85%)	1309:1325	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	40	with	expression	1129:1138	arg1	%					1324:1324	>85%	1321:1324	>85%	1321:1324	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	40	with	expression	1129:1138	arg1	branching					1295:1303	neural branching	1288:1303	neural branching	1288:1303	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	3	41	theme	cell	451:454	arg1	lineages					456:463	unwanted cell lineages	442:463	unwanted cell lineages when transplanted into a hostile injury environment	442:515	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
37099324	8	42	theme	functional	1372:1381	arg1	synaptophysin					1390:1402	the functional marker synaptophysin	1368:1402	the functional marker synaptophysin	1368:1402	The differentiated cells also expressed the functional marker synaptophysin.
37099324	10	43	theme	cell	1632:1635	arg1	growth					1637:1642	cell growth	1632:1642	cell growth	1632:1642	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	7	44	theme	intermittent	1190:1201	arg1	marker					1212:1217	an intermittent neuronal marker	1187:1217	an intermittent neuronal marker (β-III tub, day 5)	1187:1236	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	44	theme	intermittent	1190:1201	arg1	tub					1226:1228	β-III tub	1220:1228	β-III tub	1220:1228	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	0	45	theme	Rat	31:33	arg1	Cells					68:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	7	46	dep	tub	1226:1228	arg1	day					1231:1233	day 5	1231:1235	day 5	1231:1235	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	0	47	with	Engineering	14:24	arg1	Cells					68:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	6	48	attach	derived	882:888	arg1	gelatin					925:931	gelatin	925:931	gelatin	925:931	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	6	48	attach	derived	882:888	arg1	dialdehyde					904:913	alginate dialdehyde	895:913	alginate dialdehyde (ADA)	895:919	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	6	48	attach	derived	882:888	arg2	composition					853:863	An injectable composition	839:863	An injectable composition	839:863	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	6	48	attach	derived	882:888	arg1	ADA					916:918	ADA	916:918	ADA	916:918	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	0	49	theme	Mesenchymal	51:61	arg1	Cells					68:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	1	50	contain	has	202:204	arg1	system					195:200	The central nervous system	175:200	The central nervous system	175:200	The central nervous system has limited regeneration potential.
37099324	1	50	contain	has	202:204	arg2	potential					227:235	limited regeneration potential	206:235	limited regeneration potential	206:235	The central nervous system has limited regeneration potential.
37099324	2	51	theme	autologous	325:334	arg1	source					341:346	an ideal autologous cell source	316:346	an ideal autologous cell source for the regeneration of neural tissues	316:385	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	1	52	theme	regeneration	214:225	arg1	potential					227:235	limited regeneration potential	206:235	limited regeneration potential	206:235	The central nervous system has limited regeneration potential.
37099324	0	53	dep	Engineering	14:24	arg1	Role					79:82	The Role	75:82	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.	0:173	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	4	54	theme	site-specific	619:631	arg1	delivery					633:640	site-specific delivery	619:640	site-specific delivery for better survival of cells	619:669	Transplanting predifferentiated cells via an injectable carrier may aid in site-specific delivery for better survival of cells.
37099324	12	55	theme	progenitor	1999:2008	arg1	growth					2015:2020	stem/neural progenitor cell growth	1987:2020	stem/neural progenitor cell growth	1987:2020	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	10	56	theme	appropriate	1557:1567	arg1	quantities					1569:1578	appropriate quantities	1557:1578	appropriate quantities of asiatic acid specific for neural niche	1557:1620	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	7	57	theme	neuronal	1252:1259	arg1	marker					1261:1266	a mature neuronal marker	1243:1266	a mature neuronal marker (MAP-2, day 8)	1243:1281	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	57	theme	neuronal	1252:1259	arg1	MAP-2					1269:1273	MAP-2	1269:1273	MAP-2	1269:1273	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	10	58	theme	neural	1716:1721	arg1	branching					1723:1731	neural branching	1716:1731	neural branching	1716:1731	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	12	59	from	site	2152:2155	arg1	release					2116:2122	release	2116:2122	release at the cell transplantation site	2116:2155	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	10	60	theme	cell	1682:1685	arg1	survival					1687:1694	cell survival	1682:1694	cell survival	1682:1694	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	7	61	theme	marker	1163:1168	arg1	expression					1129:1138	stage-specific expression	1114:1138	stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%)	1114:1325	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	61	theme	marker	1163:1168	arg1	generation					1073:1082	the generation	1069:1082	the generation of prominent neurospheres	1069:1108	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	6	62	theme	injectable	842:851	arg1	composition					853:863	An injectable composition	839:863	An injectable composition	839:863	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	2	63	theme	cells	291:295	arg1	multipotency					242:253	The multipotency	238:253	The multipotency of adipose-derived mesenchymal stem cells (ADMSC)	238:303	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	9	64	theme	negative	1418:1425	arg1	impact					1427:1432	no negative impact	1415:1432	no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%)	1415:1498	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	7	65	dep	nestin	1171:1176	arg1	day					1179:1181	day 4	1179:1183	day 4	1179:1183	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	13	66	theme	diseases	2335:2342	arg1	therapies					2315:2323	cell-based therapies	2304:2323	cell-based therapies of neural diseases	2304:2342	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	7	67	theme	neural	1145:1150	arg1	marker					1163:1168	a neural progenitor marker	1143:1168	a neural progenitor marker (nestin, day 4)	1143:1184	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	67	theme	neural	1145:1150	arg1	nestin					1171:1176	nestin	1171:1176	nestin	1171:1176	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	12	68	theme	cell	2131:2134	arg1	site					2152:2155	the cell transplantation site	2127:2155	the cell transplantation site	2127:2155	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	11	69	theme	hydrogel	1781:1788	arg1	niche					1790:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	0	70	theme	Hydrogel	113:120	arg1	Scaffold					122:129	an Injectable, Resorbable Hydrogel Scaffold	87:129	an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin	87:172	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	9	71	theme	cell	1453:1456	arg1	survival					1458:1465	stem/progenitor cell survival	1437:1465	stem/progenitor cell survival (>95%)	1437:1472	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	9	71	theme	cell	1453:1456	arg1	%					1471:1471	>95%	1468:1471	>95%	1468:1471	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	13	72	dep	matrix	2172:2177	arg1	incorporated					2189:2200	incorporated	2189:2200	incorporated with phytomoieties	2189:2219	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	12	73	theme	growth	2094:2099	arg1	promoters					2101:2109	growth promoters	2094:2109	growth promoters	2094:2109	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	12	73	theme	growth	2094:2099	arg1	applications					2061:2072	potential applications	2051:2072	potential applications	2051:2072	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	10	74	theme	acid	1591:1594	arg1	quantities					1569:1578	appropriate quantities	1557:1578	appropriate quantities of asiatic acid specific for neural niche	1557:1620	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	2	75	theme	cell	336:339	arg1	source					341:346	an ideal autologous cell source	316:346	an ideal autologous cell source for the regeneration of neural tissues	316:385	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	7	76	theme	stage-specific	1114:1127	arg1	expression					1129:1138	stage-specific expression	1114:1138	stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%)	1114:1325	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	1	77	theme	central	179:185	arg1	system					195:200	The central nervous system	175:200	The central nervous system	175:200	The central nervous system has limited regeneration potential.
37099324	4	78	theme	cells	665:669	arg1	survival					653:660	better survival	646:660	better survival of cells	646:669	Transplanting predifferentiated cells via an injectable carrier may aid in site-specific delivery for better survival of cells.
37099324	10	79	theme	specific	1596:1603	arg1	acid					1591:1594	asiatic acid	1583:1594	asiatic acid specific for neural niche	1583:1620	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	0	80	theme	Oxidized	144:151	arg1	Alginate					153:160	Alginate	153:160	Alginate	153:160	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	13	81	theme	cell	2278:2281	arg1	matrix					2172:2177	the matrix itself or incorporated with phytomoieties	2168:2219	the matrix itself or incorporated with phytomoieties	2168:2219	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	13	81	theme	cell	2278:2281	arg1	vehicle					2292:2298	a potential minimally invasive injectable cell delivery vehicle	2236:2298	a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases	2236:2342	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	5	82	theme	injectable	717:726	arg1	system					737:742	an appropriate injectable hydrogel system	702:742	an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation	702:806	Here, we focus on identifying an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation for neural tissue engineering.
37099324	9	83	dep	two-dimensional	1515:1529	arg1	2D					1532:1533	2D	1532:1533	2D	1532:1533	There was no negative impact on stem/progenitor cell survival (>95%) or differentiation (∼90%) as compared to two-dimensional (2D) culture.
37099324	13	84	theme	neural	2328:2333	arg1	diseases					2335:2342	neural diseases	2328:2342	neural diseases	2328:2342	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	2	85	theme	mesenchymal	274:284	arg1	ADMSC					298:302	ADMSC	298:302	ADMSC	298:302	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	2	85	theme	mesenchymal	274:284	arg1	cells					291:295	adipose-derived mesenchymal stem cells	258:295	adipose-derived mesenchymal stem cells (ADMSC)	258:303	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	1	86	theme	nervous	187:193	arg1	system					195:200	The central nervous system	175:200	The central nervous system	175:200	The central nervous system has limited regeneration potential.
37099324	13	87	theme	potential	2238:2246	arg1	matrix					2172:2177	the matrix itself or incorporated with phytomoieties	2168:2219	the matrix itself or incorporated with phytomoieties	2168:2219	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	13	87	theme	potential	2238:2246	arg1	vehicle					2292:2298	a potential minimally invasive injectable cell delivery vehicle	2236:2298	a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases	2236:2342	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	12	88	theme	ADA-gelatin	1894:1904	arg1	hydrogel					1906:1913	ADA-gelatin hydrogel	1894:1913	ADA-gelatin hydrogel by itself	1894:1923	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	7	89	theme	neural	1033:1038	arg1	progenitors					1040:1050	neural progenitors	1033:1050	neural progenitors	1033:1050	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	90	theme	ADMSCs	1023:1028	arg1	proliferation/differentiation					990:1018	proliferation/differentiation	990:1018	proliferation/differentiation of ADMSCs to neural progenitors	990:1050	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	91	dep	MAP-2	1269:1273	arg1	day					1276:1278	day 8	1276:1280	day 8	1276:1280	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	13	92	theme	cell-based	2304:2313	arg1	therapies					2315:2323	cell-based therapies	2304:2323	cell-based therapies of neural diseases	2304:2342	In short, the matrix itself or incorporated with phytomoieties could serve as a potential minimally invasive injectable cell delivery vehicle for cell-based therapies of neural diseases.
37099324	2	93	link	adipose-derived	258:272	arg1	ADMSC					298:302	ADMSC	298:302	ADMSC	298:302	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	2	93	link	adipose-derived	258:272	arg1	cells					291:295	adipose-derived mesenchymal stem cells	258:295	adipose-derived mesenchymal stem cells (ADMSC)	258:303	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	3	94	theme	hostile	490:496	arg1	environment					505:515	a hostile injury environment	488:515	a hostile injury environment	488:515	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
37099324	3	95	theme	unwanted	442:449	arg1	lineages					456:463	unwanted cell lineages	442:463	unwanted cell lineages when transplanted into a hostile injury environment	442:515	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
37099324	0	96	theme	Tissue	7:12	arg1	Engineering					14:24	Neural Tissue Engineering	0:24	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.	0:173	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	11	97	theme	interconnected	1759:1772	arg1	niche					1790:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche	1749:1794	Optimized interconnected porous hydrogel niche exhibited rapid gelation (3 min) and self-healing properties mimicking native neural tissue.
37099324	0	98	attach	Derived	131:137	arg1	Gelatin					166:172	Gelatin	166:172	Gelatin	166:172	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	0	98	attach	Derived	131:137	arg1	Alginate					153:160	Alginate	153:160	Alginate	153:160	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	0	98	attach	Derived	131:137	arg2	Scaffold					122:129	an Injectable, Resorbable Hydrogel Scaffold	87:129	an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin	87:172	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	8	99	theme	differentiated	1332:1345	arg1	cells					1347:1351	The differentiated cells	1328:1351	The differentiated cells	1328:1351	The differentiated cells also expressed the functional marker synaptophysin.
37099324	3	100	theme	differentiation	421:435	arg1	disadvantage					530:541	a serious disadvantage	520:541	a serious disadvantage	520:541	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
37099324	3	100	theme	differentiation	421:435	arg1	likelihood					401:410	the likelihood	397:410	the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment	397:515	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
37099324	7	101	theme	neuronal	1203:1210	arg1	marker					1212:1217	an intermittent neuronal marker	1187:1217	an intermittent neuronal marker (β-III tub, day 5)	1187:1236	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	101	theme	neuronal	1203:1210	arg1	tub					1226:1228	β-III tub	1220:1228	β-III tub	1220:1228	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	102	theme	neural	1288:1293	arg1	branching					1295:1303	neural branching	1288:1303	neural branching	1288:1303	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	4	103	theme	injectable	589:598	arg1	carrier					600:606	an injectable carrier	586:606	an injectable carrier	586:606	Transplanting predifferentiated cells via an injectable carrier may aid in site-specific delivery for better survival of cells.
37099324	5	104	theme	tissue	819:824	arg1	engineering					826:836	neural tissue engineering	812:836	neural tissue engineering	812:836	Here, we focus on identifying an appropriate injectable hydrogel system that favors stem/progenitor cell attachment and differentiation for neural tissue engineering.
37099324	10	105	dep	supported	1622:1630	arg1	%					1700:1700	>90%	1697:1700	>90%	1697:1700	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	0	106	theme	Stem	63:66	arg1	Cells					68:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Rat Adipose-Derived Mesenchymal Stem Cells	31:72	Neural Tissue Engineering with Rat Adipose-Derived Mesenchymal Stem Cells: The Role of an Injectable, Resorbable Hydrogel Scaffold Derived from Oxidized Alginate and Gelatin.
37099324	6	107	theme	alginate	895:902	arg1	dialdehyde					904:913	alginate dialdehyde	895:913	alginate dialdehyde (ADA)	895:919	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	6	107	theme	alginate	895:902	arg1	ADA					916:918	ADA	916:918	ADA	916:918	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	4	108	theme	predifferentiated	558:574	arg1	cells					576:580	predifferentiated cells	558:580	predifferentiated cells	558:580	Transplanting predifferentiated cells via an injectable carrier may aid in site-specific delivery for better survival of cells.
37099324	8	109	theme	marker	1383:1388	arg1	synaptophysin					1390:1402	the functional marker synaptophysin	1368:1402	the functional marker synaptophysin	1368:1402	The differentiated cells also expressed the functional marker synaptophysin.
37099324	3	110	theme	injury	498:503	arg1	environment					505:515	a hostile injury environment	488:515	a hostile injury environment	488:515	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
37099324	2	111	theme	ideal	319:323	arg1	source					341:346	an ideal autologous cell source	316:346	an ideal autologous cell source for the regeneration of neural tissues	316:385	The multipotency of adipose-derived mesenchymal stem cells (ADMSC) makes them an ideal autologous cell source for the regeneration of neural tissues.
37099324	12	112	theme	cell	2010:2013	arg1	growth					2015:2020	stem/neural progenitor cell growth	1987:2020	stem/neural progenitor cell growth	1987:2020	Both ADA-gelatin hydrogel by itself and that incorporated with asiatic acid were found to support stem/neural progenitor cell growth and differentiation and have potential applications as antioxidants and growth promoters upon release at the cell transplantation site.
37099324	6	113	theme	hydrogel	872:879	arg1	composition					853:863	An injectable composition	839:863	An injectable composition	839:863	An injectable composition of the hydrogel, derived from alginate dialdehyde (ADA) and gelatin, was formulated for this purpose.
37099324	7	114	theme	marker	1261:1266	arg1	expression					1129:1138	stage-specific expression	1114:1138	stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%)	1114:1325	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	7	114	theme	marker	1261:1266	arg1	generation					1073:1082	the generation	1069:1082	the generation of prominent neurospheres	1069:1108	This hydrogel promoted proliferation/differentiation of ADMSCs to neural progenitors, visualized from the generation of prominent neurospheres and stage-specific expression of a neural progenitor marker (nestin, day 4), an intermittent neuronal marker (β-III tub, day 5), and a mature neuronal marker (MAP-2, day 8) with neural branching and networking (>85%).
37099324	10	115	theme	neural	1609:1614	arg1	niche					1616:1620	neural niche	1609:1620	neural niche	1609:1620	Addition of appropriate quantities of asiatic acid specific for neural niche supported cell growth and differentiation without affecting cell survival (>90%) and improved neural branching and elongation.
37099324	4	116	theme	better	646:651	arg1	survival					653:660	better survival	646:660	better survival of cells	646:669	Transplanting predifferentiated cells via an injectable carrier may aid in site-specific delivery for better survival of cells.
37099324	3	117	theme	serious	522:528	arg1	disadvantage					530:541	a serious disadvantage	520:541	a serious disadvantage	520:541	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
37099324	3	117	theme	serious	522:528	arg1	likelihood					401:410	the likelihood	397:410	the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment	397:515	However, the likelihood of their differentiation into unwanted cell lineages when transplanted into a hostile injury environment is a serious disadvantage.
36825081	0	0	theme	Intestinal	85:94	arg1	Function					104:111	Intestinal Barrier Function	85:111	Intestinal Barrier Function	85:111	Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function.
36825081	4	1	from	mechanisms	615:624	arg1	models					698:703	colitis mouse models	684:703	colitis mouse models	684:703	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	5	2	theme	disease	773:779	arg1	symptoms					781:788	the disease symptoms	769:788	the disease symptoms	769:788	The results showed that all the treatment groups could improve the disease symptoms, and the combined administration showed the best effect.
36825081	8	3	theme	treatment	1301:1309	arg1	groups					1311:1316	all the treatment groups	1293:1316	all the treatment groups	1293:1316	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	6	4	theme	occludin	1027:1034	arg1	expression					1001:1010	the expression	997:1010	the expression of E-cadherin, occludin, ZO-1, and MUC2	997:1050	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	6	5	theme	intestinal	1066:1075	arg1	permeability					1077:1088	intestinal permeability	1066:1088	intestinal permeability	1066:1088	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	10	6	theme	treatment	1628:1636	arg1	groups					1638:1643	all the treatment groups	1620:1643	all the treatment groups	1620:1643	Finally, all the treatment groups modulated intestinal microbiota composition, increased the proportion of beneficial microbiota, and decreased the proportion of microbiota.
36825081	10	7	theme	microbiota	1666:1675	arg1	composition					1677:1687	intestinal microbiota composition	1655:1687	intestinal microbiota composition	1655:1687	Finally, all the treatment groups modulated intestinal microbiota composition, increased the proportion of beneficial microbiota, and decreased the proportion of microbiota.
36825081	1	8	theme	traditional	160:170	arg1	medicine					180:187	traditional Chinese medicine	160:187	traditional Chinese medicine indigo naturalis	160:204	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	2	9	from	profiles	317:324	arg1	anti-inflammation					329:345	anti-inflammation	329:345	anti-inflammation	329:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	7	10	theme	cytokines	1153:1161	arg1	expression					1139:1148	the expression	1135:1148	the expression of cytokines, including TNF-α, IFN-γ, IL-12, IL-23, and IL-17A	1135:1211	The treatment groups significantly improved the expression of cytokines, including TNF-α, IFN-γ, IL-12, IL-23, and IL-17A, and indirubin presented the most potent anti-inflammatory effect.
36825081	4	11	from	effect	604:609	arg1	models					698:703	colitis mouse models	684:703	colitis mouse models	684:703	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	6	12	theme	combination	907:917	arg1	group					919:923	the combination group	903:923	the combination group	903:923	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	8	13	theme	cells	1357:1361	arg1	infiltration					1330:1341	the infiltration	1326:1341	the infiltration of the immune cells in intestinal lamina propria	1326:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	8	13	theme	cells	1357:1361	arg1	production					1400:1409	the production	1396:1409	the production of ROS/RNS	1396:1420	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	4	14	theme	indirubin	671:679	arg1	administration					642:655	the combined administration	629:655	the combined administration of indigo and indirubin	629:679	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	6	15	theme	E-cadherin	1015:1024	arg1	expression					1001:1010	the expression	997:1010	the expression of E-cadherin, occludin, ZO-1, and MUC2	997:1050	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	0	16	theme	Barrier	96:102	arg1	Function					104:111	Intestinal Barrier Function	85:111	Intestinal Barrier Function	85:111	Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function.
36825081	10	17	theme	microbiota	1729:1738	arg1	proportion					1704:1713	the proportion	1700:1713	the proportion of beneficial microbiota	1700:1738	Finally, all the treatment groups modulated intestinal microbiota composition, increased the proportion of beneficial microbiota, and decreased the proportion of microbiota.
36825081	7	18	theme	potent	1247:1252	arg1	effect					1272:1277	the most potent anti-inflammatory effect	1238:1277	the most potent anti-inflammatory effect	1238:1277	The treatment groups significantly improved the expression of cytokines, including TNF-α, IFN-γ, IL-12, IL-23, and IL-17A, and indirubin presented the most potent anti-inflammatory effect.
36825081	8	19	theme	immune	1350:1355	arg1	cells					1357:1361	the immune cells	1346:1361	the immune cells in intestinal lamina propria	1346:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	11	20	theme	intestinal	1864:1873	arg1	function					1883:1890	the intestinal barrier function	1860:1890	the intestinal barrier function	1860:1890	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	3	21	theme	combined	498:505	arg1	administration					507:520	its combined administration	494:520	its combined administration's integrated advantages for UC	494:551	Thus, exploring its combined administration's integrated advantages for UC is critical.
36825081	4	22	theme	combined	633:640	arg1	administration					642:655	the combined administration	629:655	the combined administration of indigo and indirubin	629:679	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	8	23	theme	intestinal	1366:1375	arg1	propria					1384:1390	intestinal lamina propria	1366:1390	intestinal lamina propria	1366:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	4	24	theme	colitis	684:690	arg1	models					698:703	colitis mouse models	684:703	colitis mouse models	684:703	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	1	25	theme	Chinese	172:178	arg1	medicine					180:187	traditional Chinese medicine	160:187	traditional Chinese medicine indigo naturalis	160:204	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	10	26	theme	intestinal	1655:1664	arg1	composition					1677:1687	intestinal microbiota composition	1655:1687	intestinal microbiota composition	1655:1687	Finally, all the treatment groups modulated intestinal microbiota composition, increased the proportion of beneficial microbiota, and decreased the proportion of microbiota.
36825081	2	27	theme	oxidative	401:409	arg1	regulation					418:427	oxidative stress regulation	401:427	oxidative stress regulation	401:427	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	27	theme	oxidative	401:409	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	8	28	from	propria	1384:1390	arg1	infiltration					1330:1341	the infiltration	1326:1341	the infiltration of the immune cells in intestinal lamina propria	1326:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	8	28	from	propria	1384:1390	arg1	production					1400:1409	the production	1396:1409	the production of ROS/RNS	1396:1420	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	8	29	from	production	1400:1409	arg1	propria					1384:1390	intestinal lamina propria	1366:1390	intestinal lamina propria	1366:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	1	30	theme	indigo	189:194	arg1	naturalis					196:204	traditional Chinese medicine indigo naturalis	160:204	traditional Chinese medicine indigo naturalis	160:204	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	0	31	theme	Combination	0:10	arg1	Therapy					12:18	Combination Therapy	0:18	Combination Therapy with Indigo and Indirubin for Ulcerative Colitis	0:67	Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function.
36825081	8	32	from	infiltration	1330:1341	arg1	propria					1384:1390	intestinal lamina propria	1366:1390	intestinal lamina propria	1366:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	2	33	theme	distinctive	305:315	arg1	regulation					355:364	immune regulation	348:364	immune regulation	348:364	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	33	theme	distinctive	305:315	arg1	regulation					389:398	intestinal microbiota regulation	367:398	intestinal microbiota regulation	367:398	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	33	theme	distinctive	305:315	arg1	repair					453:458	intestinal mucosal repair	434:458	intestinal mucosal repair for UC treatment	434:475	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	33	theme	distinctive	305:315	arg1	regulation					418:427	oxidative stress regulation	401:427	oxidative stress regulation	401:427	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	33	theme	distinctive	305:315	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	1	34	theme	naturalis	196:204	arg1	molecules					147:155	the active molecules	136:155	the active molecules of traditional Chinese medicine indigo naturalis	136:204	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	1	34	theme	naturalis	196:204	arg1	Indigo					114:119	Indigo	114:119	Indigo	114:119	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	7	35	theme	anti-inflammatory	1254:1270	arg1	effect					1272:1277	the most potent anti-inflammatory effect	1238:1277	the most potent anti-inflammatory effect	1238:1277	The treatment groups significantly improved the expression of cytokines, including TNF-α, IFN-γ, IL-12, IL-23, and IL-17A, and indirubin presented the most potent anti-inflammatory effect.
36825081	10	36	theme	microbiota	1773:1782	arg1	proportion					1759:1768	the proportion	1755:1768	the proportion of microbiota	1755:1782	Finally, all the treatment groups modulated intestinal microbiota composition, increased the proportion of beneficial microbiota, and decreased the proportion of microbiota.
36825081	2	37	theme	intestinal	434:443	arg1	repair					453:458	intestinal mucosal repair	434:458	intestinal mucosal repair for UC treatment	434:475	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	37	theme	intestinal	434:443	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	38	theme	mucosal	445:451	arg1	repair					453:458	intestinal mucosal repair	434:458	intestinal mucosal repair for UC treatment	434:475	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	38	theme	mucosal	445:451	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	7	39	theme	treatment	1095:1103	arg1	groups					1105:1110	The treatment groups	1091:1110	The treatment groups	1091:1110	The treatment groups significantly improved the expression of cytokines, including TNF-α, IFN-γ, IL-12, IL-23, and IL-17A, and indirubin presented the most potent anti-inflammatory effect.
36825081	4	40	dep	effect	604:609	arg1	the					600:602	the	600:602	the	600:602	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	3	41	theme	integrated	524:533	arg1	advantages					535:544	its combined administration's integrated advantages	494:544	its combined administration's integrated advantages for UC	494:551	Thus, exploring its combined administration's integrated advantages for UC is critical.
36825081	1	42	theme	active	140:145	arg1	molecules					147:155	the active molecules	136:155	the active molecules of traditional Chinese medicine indigo naturalis	136:204	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	1	42	theme	active	140:145	arg1	Indigo					114:119	Indigo	114:119	Indigo	114:119	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	11	43	theme	barrier	1875:1881	arg1	function					1883:1890	the intestinal barrier function	1860:1890	the intestinal barrier function	1860:1890	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	4	44	theme	indigo	660:665	arg1	administration					642:655	the combined administration	629:655	the combined administration of indigo and indirubin	629:679	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	9	45	theme	combination	1572:1582	arg1	group					1584:1588	the combination group	1568:1588	the combination group	1568:1588	Notably, indigo exhibited a more substantial capacity to regulate natural killer (NK) cells, ILC3, neutrophils, and dendritic cells, followed by the combination group and indirubin alone.
36825081	1	46	theme	therapeutic	213:223	arg1	activity					225:232	therapeutic activity	213:232	therapeutic activity for ulcerative colitis (UC)	213:260	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	2	47	theme	stress	411:416	arg1	regulation					418:427	oxidative stress regulation	401:427	oxidative stress regulation	401:427	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	47	theme	stress	411:416	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	11	48	theme	regulation	2042:2051	arg1	advantage					2053:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	11	49	theme	intestinal	1970:1979	arg1	microbiota					1981:1990	intestinal microbiota	1970:1990	intestinal microbiota	1970:1990	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	11	50	theme	anti-inflammatory	1948:1964	arg1	indirubin					1938:1946	the indirubin anti-inflammatory	1934:1964	the indirubin anti-inflammatory	1934:1964	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	6	51	theme	MUC2	1047:1050	arg1	expression					1001:1010	the expression	997:1010	the expression of E-cadherin, occludin, ZO-1, and MUC2	997:1050	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	0	52	theme	Ulcerative	50:59	arg1	Colitis					61:67	Ulcerative Colitis	50:67	Ulcerative Colitis	50:67	Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function.
36825081	2	53	contain	have	300:303	arg1	indirubin					274:282	indirubin	274:282	indirubin	274:282	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	53	contain	have	300:303	arg2	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	53	contain	have	300:303	arg2	regulation					389:398	intestinal microbiota regulation	367:398	intestinal microbiota regulation	367:398	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	53	contain	have	300:303	arg1	isomers					288:294	isomers	288:294	isomers	288:294	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	53	contain	have	300:303	arg2	regulation					355:364	immune regulation	348:364	immune regulation	348:364	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	53	contain	have	300:303	arg2	repair					453:458	intestinal mucosal repair	434:458	intestinal mucosal repair for UC treatment	434:475	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	53	contain	have	300:303	arg2	regulation					418:427	oxidative stress regulation	401:427	oxidative stress regulation	401:427	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	53	contain	have	300:303	arg1	Indigo					263:268	Indigo	263:268	Indigo	263:268	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	9	54	theme	natural	1489:1495	arg1	NK					1505:1506	NK	1505:1506	NK	1505:1506	Notably, indigo exhibited a more substantial capacity to regulate natural killer (NK) cells, ILC3, neutrophils, and dendritic cells, followed by the combination group and indirubin alone.
36825081	9	54	theme	natural	1489:1495	arg1	killer					1497:1502	natural killer	1489:1502	natural killer (NK) cells	1489:1513	Notably, indigo exhibited a more substantial capacity to regulate natural killer (NK) cells, ILC3, neutrophils, and dendritic cells, followed by the combination group and indirubin alone.
36825081	8	55	theme	lamina	1377:1382	arg1	propria					1384:1390	intestinal lamina propria	1366:1390	intestinal lamina propria	1366:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	2	56	theme	immune	348:353	arg1	regulation					355:364	immune regulation	348:364	immune regulation	348:364	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	56	theme	immune	348:353	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	5	57	theme	combined	799:806	arg1	administration					808:821	the combined administration	795:821	the combined administration	795:821	The results showed that all the treatment groups could improve the disease symptoms, and the combined administration showed the best effect.
36825081	6	58	theme	intestinal	955:964	arg1	function					974:981	intestinal barrier function	955:981	intestinal barrier function	955:981	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	8	59	theme	ROS/RNS	1414:1420	arg1	infiltration					1330:1341	the infiltration	1326:1341	the infiltration of the immune cells in intestinal lamina propria	1326:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	8	59	theme	ROS/RNS	1414:1420	arg1	production					1400:1409	the production	1396:1409	the production of ROS/RNS	1396:1420	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	0	60	with	Therapy	12:18	arg1	Indirubin					36:44	Indirubin	36:44	Indirubin	36:44	Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function.
36825081	0	60	with	Therapy	12:18	arg1	Indigo					25:30	Indigo	25:30	Indigo	25:30	Combination Therapy with Indigo and Indirubin for Ulcerative Colitis via Reinforcing Intestinal Barrier Function.
36825081	11	61	theme	immune	2023:2028	arg1	advantage					2053:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	10	62	theme	beneficial	1718:1727	arg1	microbiota					1729:1738	beneficial microbiota	1718:1738	beneficial microbiota	1718:1738	Finally, all the treatment groups modulated intestinal microbiota composition, increased the proportion of beneficial microbiota, and decreased the proportion of microbiota.
36825081	11	63	theme	ROS/RNS	2034:2040	arg1	advantage					2053:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	4	64	theme	mouse	692:696	arg1	models					698:703	colitis mouse models	684:703	colitis mouse models	684:703	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	8	65	from	cells	1357:1361	arg1	propria					1384:1390	intestinal lamina propria	1366:1390	intestinal lamina propria	1366:1390	Furthermore, all the treatment groups reduced the infiltration of the immune cells in intestinal lamina propria and the production of ROS/RNS.
36825081	1	66	theme	ulcerative	238:247	arg1	colitis					249:255	ulcerative colitis	238:255	ulcerative colitis (UC)	238:260	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	1	66	theme	ulcerative	238:247	arg1	UC					258:259	UC	258:259	UC	258:259	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	9	67	theme	killer	1497:1502	arg1	cells					1509:1513	natural killer (NK) cells	1489:1513	natural killer (NK) cells	1489:1513	Notably, indigo exhibited a more substantial capacity to regulate natural killer (NK) cells, ILC3, neutrophils, and dendritic cells, followed by the combination group and indirubin alone.
36825081	11	68	theme	indigo	2016:2021	arg1	advantage					2053:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	indigo immune and ROS/RNS regulation advantage	2016:2061	Our results indicated that indigo and indirubin synergistically reinforced the intestinal barrier function, which may be associated with integrating the indirubin anti-inflammatory and intestinal microbiota regulating strength and indigo immune and ROS/RNS regulation advantage.
36825081	4	69	theme	administration	642:655	arg1	mechanisms					615:624	mechanisms	615:624	mechanisms	615:624	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	4	69	theme	administration	642:655	arg1	effect					604:609	effect	604:609	effect	604:609	This study is aimed at clarifying the effect and mechanisms of the combined administration of indigo and indirubin on colitis mouse models.
36825081	2	70	theme	microbiota	378:387	arg1	regulation					389:398	intestinal microbiota regulation	367:398	intestinal microbiota regulation	367:398	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	70	theme	microbiota	378:387	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	1	71	theme	medicine	180:187	arg1	naturalis					196:204	traditional Chinese medicine indigo naturalis	160:204	traditional Chinese medicine indigo naturalis	160:204	Indigo and indirubin, the active molecules of traditional Chinese medicine indigo naturalis, exert therapeutic activity for ulcerative colitis (UC).
36825081	6	72	theme	ZO-1	1037:1040	arg1	expression					1001:1010	the expression	997:1010	the expression of E-cadherin, occludin, ZO-1, and MUC2	997:1050	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	9	73	theme	substantial	1456:1466	arg1	capacity					1468:1475	a more substantial capacity	1449:1475	a more substantial capacity to regulate natural killer (NK) cells, ILC3, neutrophils, and dendritic cells	1449:1553	Notably, indigo exhibited a more substantial capacity to regulate natural killer (NK) cells, ILC3, neutrophils, and dendritic cells, followed by the combination group and indirubin alone.
36825081	5	74	theme	best	834:837	arg1	effect					839:844	the best effect	830:844	the best effect	830:844	The results showed that all the treatment groups could improve the disease symptoms, and the combined administration showed the best effect.
36825081	9	75	theme	dendritic	1539:1547	arg1	cells					1549:1553	dendritic cells	1539:1553	dendritic cells	1539:1553	Notably, indigo exhibited a more substantial capacity to regulate natural killer (NK) cells, ILC3, neutrophils, and dendritic cells, followed by the combination group and indirubin alone.
36825081	2	76	theme	intestinal	367:376	arg1	regulation					389:398	intestinal microbiota regulation	367:398	intestinal microbiota regulation	367:398	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	2	76	theme	intestinal	367:376	arg1	profiles					317:324	distinctive profiles	305:324	distinctive profiles in anti-inflammation	305:345	Indigo and indirubin are isomers and have distinctive profiles in anti-inflammation, immune regulation, intestinal microbiota regulation, oxidative stress regulation, and intestinal mucosal repair for UC treatment.
36825081	6	77	theme	barrier	966:972	arg1	function					974:981	intestinal barrier function	955:981	intestinal barrier function	955:981	Additionally, compared with indigo and indirubin alone, the combination group could significantly reinforce intestinal barrier function by increasing the expression of E-cadherin, occludin, ZO-1, and MUC2 and improving intestinal permeability.
36825081	5	78	theme	treatment	738:746	arg1	groups					748:753	all the treatment groups	730:753	all the treatment groups	730:753	The results showed that all the treatment groups could improve the disease symptoms, and the combined administration showed the best effect.
37061003	7	0	theme	melanoma	1052:1059	arg1	cells					1061:1065	human melanoma cells	1046:1065	human melanoma cells	1046:1065	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	2	1	theme	assembly	307:314	arg1	Dysregulation					287:299	Dysregulation	287:299	Dysregulation of HS assembly	287:314	Dysregulation of HS assembly has been implicated in pathophysiological conditions, such as tumorigenesis and rare genetic disorders.
37061003	9	2	theme	enzymes	1355:1361	arg1	expression					1332:1341	expression	1332:1341	expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1	1332:1430	Additionally, RNA sequencing revealed that TFCP2 regulates expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1.
37061003	10	3	from	knockdown	1553:1561	arg1	cells					1599:1603	TFCP2 mutant cells	1586:1603	TFCP2 mutant cells	1586:1603	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	10	4	theme	SULF1	1531:1535	arg1	expression					1537:1546	SULF1 expression	1531:1546	SULF1 expression	1531:1546	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	1	5	theme	many	220:223	arg1	signaling					260:268	cell signaling	255:268	cell signaling	255:268	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	1	5	theme	many	220:223	arg1	processes					234:242	many cellular processes	220:242	many cellular processes	220:242	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	1	5	theme	many	220:223	arg1	development					274:284	development	274:284	development	274:284	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	8	6	theme	knockout	1103:1110	arg1	cells					1112:1116	TFCP2 knockout cells	1097:1116	TFCP2 knockout cells	1097:1116	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	8	7	theme	surface	1179:1185	arg1	HS					1187:1188	cell surface HS	1174:1188	cell surface HS	1174:1188	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	9	8	theme	multiple	1346:1353	arg1	enzymes					1355:1361	multiple enzymes	1346:1361	multiple enzymes	1346:1361	Additionally, RNA sequencing revealed that TFCP2 regulates expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1.
37061003	9	8	theme	multiple	1346:1353	arg1	endosulfatase					1411:1423	the secreted endosulfatase	1398:1423	the secreted endosulfatase	1398:1423	Additionally, RNA sequencing revealed that TFCP2 regulates expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1.
37061003	5	9	theme	novel	826:830	arg1	factors					843:849	novel regulatory factors	826:849	novel regulatory factors of HS biosynthesis	826:868	To address this gap in knowledge, we recently performed genome-wide CRISPR/Cas9 screens to identify novel regulatory factors of HS biosynthesis.
37061003	11	10	theme	possible	1760:1767	arg1	target					1769:1774	a possible target	1758:1774	a possible target to slow melanoma growth	1758:1798	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	11	10	theme	possible	1760:1767	arg1	interactions					1742:1753	HS-protein interactions	1731:1753	HS-protein interactions	1731:1753	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	3	11	theme	large	540:544	arg1	group					546:550	a large group	538:550	a large group of biosynthetic enzymes	538:574	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	3	11	theme	large	540:544	arg1	enzymes					568:574	biosynthetic enzymes	555:574	biosynthetic enzymes	555:574	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	8	12	theme	fibroblast	1138:1147	arg1	factor					1156:1161	fibroblast growth factor	1138:1161	decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells	1128:1270	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	9	13	theme	secreted	1402:1409	arg1	SULF1					1426:1430	SULF1	1426:1430	SULF1	1426:1430	Additionally, RNA sequencing revealed that TFCP2 regulates expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1.
37061003	9	13	theme	secreted	1402:1409	arg1	endosulfatase					1411:1423	the secreted endosulfatase	1398:1423	the secreted endosulfatase	1398:1423	Additionally, RNA sequencing revealed that TFCP2 regulates expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1.
37061003	8	14	theme	factor	1156:1161	arg1	binding					1163:1169	decreased fibroblast growth factor binding	1128:1169	decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells	1128:1270	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	6	15	theme	globin	915:920	arg1	hit					960:962	a top hit	954:962	a top hit	954:962	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	6	15	theme	globin	915:920	arg1	TFCP2					944:948	TFCP2	944:948	TFCP2	944:948	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	6	15	theme	globin	915:920	arg1	factor					936:941	the alpha globin transcription factor	905:941	the alpha globin transcription factor	905:941	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	11	16	theme	melanoma	1784:1791	arg1	growth					1793:1798	slow melanoma growth	1779:1798	slow melanoma growth	1779:1798	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	3	17	theme	enzymes	568:574	arg1	group					546:550	a large group	538:550	a large group of biosynthetic enzymes	538:574	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	3	17	theme	enzymes	568:574	arg1	enzymes					568:574	biosynthetic enzymes	555:574	biosynthetic enzymes	555:574	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	1	18	theme	animal	183:188	arg1	cells					190:194	all animal cells	179:194	all animal cells	179:194	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	4	19	theme	assembly	669:676	arg1	regulation					652:661	the regulation	648:661	the regulation of HS assembly across diverse tissue types and disease states	648:723	While much is known about its biosynthesis, little is understood about the regulation of HS assembly across diverse tissue types and disease states.
37061003	10	20	theme	SULF1	1566:1570	arg1	expression					1572:1581	SULF1 expression	1566:1581	SULF1 expression in TFCP2 mutant cells	1566:1603	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	4	21	theme	diverse	685:691	arg1	types					700:704	diverse tissue types	685:704	diverse tissue types	685:704	While much is known about its biosynthesis, little is understood about the regulation of HS assembly across diverse tissue types and disease states.
37061003	8	22	theme	cell	1233:1236	arg1	growth					1238:1243	slowed cell growth	1226:1243	slowed cell growth	1226:1243	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	11	23	theme	transcriptional	1685:1699	arg1	regulator					1701:1709	a novel transcriptional regulator	1677:1709	a novel transcriptional regulator of HS	1677:1715	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	11	23	theme	transcriptional	1685:1699	arg1	TFCP2					1668:1672	TFCP2	1668:1672	TFCP2	1668:1672	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	0	24	theme	assembly	56:63	arg1	TFCP2					0:4	TFCP2	0:4	TFCP2	0:4	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	0	24	theme	assembly	56:63	arg1	regulator					27:35	a transcriptional regulator	9:35	a transcriptional regulator of heparan sulfate assembly and melanoma cell growth	9:88	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	5	25	theme	HS	854:855	arg1	biosynthesis					857:868	HS biosynthesis	854:868	HS biosynthesis	854:868	To address this gap in knowledge, we recently performed genome-wide CRISPR/Cas9 screens to identify novel regulatory factors of HS biosynthesis.
37061003	6	26	theme	top	956:958	arg1	hit					960:962	a top hit	954:962	a top hit	954:962	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	6	26	theme	top	956:958	arg1	factor					936:941	the alpha globin transcription factor	905:941	the alpha globin transcription factor	905:941	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	8	27	theme	wildtype	1257:1264	arg1	cells					1266:1270	wildtype cells	1257:1270	wildtype cells	1257:1270	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	11	28	theme	HS-protein	1731:1740	arg1	target					1769:1774	a possible target	1758:1774	a possible target to slow melanoma growth	1758:1798	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	11	28	theme	HS-protein	1731:1740	arg1	interactions					1742:1753	HS-protein interactions	1731:1753	HS-protein interactions	1731:1753	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	0	29	theme	melanoma	69:76	arg1	growth					83:88	melanoma cell growth	69:88	melanoma cell growth	69:88	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	0	30	theme	growth	83:88	arg1	TFCP2					0:4	TFCP2	0:4	TFCP2	0:4	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	0	30	theme	growth	83:88	arg1	regulator					27:35	a transcriptional regulator	9:35	a transcriptional regulator of heparan sulfate assembly and melanoma cell growth	9:88	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	10	31	theme	melanoma	1614:1621	arg1	growth					1628:1633	melanoma cell growth	1614:1633	melanoma cell growth	1614:1633	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	2	32	theme	pathophysiological	339:356	arg1	conditions					358:367	pathophysiological conditions	339:367	pathophysiological conditions	339:367	Dysregulation of HS assembly has been implicated in pathophysiological conditions, such as tumorigenesis and rare genetic disorders.
37061003	2	32	theme	pathophysiological	339:356	arg1	disorders					409:417	rare genetic disorders	396:417	rare genetic disorders	396:417	Dysregulation of HS assembly has been implicated in pathophysiological conditions, such as tumorigenesis and rare genetic disorders.
37061003	2	32	theme	pathophysiological	339:356	arg1	tumorigenesis					378:390	tumorigenesis	378:390	tumorigenesis	378:390	Dysregulation of HS assembly has been implicated in pathophysiological conditions, such as tumorigenesis and rare genetic disorders.
37061003	1	33	theme	cellular	225:232	arg1	signaling					260:268	cell signaling	255:268	cell signaling	255:268	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	1	33	theme	cellular	225:232	arg1	processes					234:242	many cellular processes	220:242	many cellular processes	220:242	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	1	33	theme	cellular	225:232	arg1	development					274:284	development	274:284	development	274:284	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	2	34	theme	genetic	401:407	arg1	disorders					409:417	rare genetic disorders	396:417	rare genetic disorders	396:417	Dysregulation of HS assembly has been implicated in pathophysiological conditions, such as tumorigenesis and rare genetic disorders.
37061003	4	35	theme	disease	710:716	arg1	states					718:723	disease states	710:723	disease states	710:723	While much is known about its biosynthesis, little is understood about the regulation of HS assembly across diverse tissue types and disease states.
37061003	3	36	theme	HS	420:421	arg1	biosynthesis					423:434	HS biosynthesis	420:434	HS biosynthesis	420:434	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	7	37	theme	CRISPR/Cas9	1077:1087	arg1	system					1089:1094	the CRISPR/Cas9 system	1073:1094	the CRISPR/Cas9 system	1073:1094	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	1	38	theme	cell	255:258	arg1	signaling					260:268	cell signaling	255:268	cell signaling	255:268	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	10	39	from	cells	1599:1603	arg1	knockdown					1553:1561	knockdown	1553:1561	knockdown of SULF1 expression in TFCP2 mutant cells	1553:1603	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	9	40	theme	RNA	1287:1289	arg1	sequencing					1291:1300	RNA sequencing	1287:1300	RNA sequencing	1287:1300	Additionally, RNA sequencing revealed that TFCP2 regulates expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1.
37061003	3	41	theme	driven	461:466	arg1	manner					468:473	a non-template driven manner	446:473	a non-template driven manner in the endoplasmic reticulum and Golgi	446:512	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	0	42	theme	heparan	40:46	arg1	assembly					56:63	heparan sulfate assembly	40:63	heparan sulfate assembly	40:63	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	7	43	theme	TFCP2	1026:1030	arg1	expression					1032:1041	TFCP2 expression	1026:1041	TFCP2 expression in human melanoma cells	1026:1065	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	10	44	theme	TFCP2	1462:1466	arg1	activity					1468:1475	TFCP2 activity	1462:1475	TFCP2 activity	1462:1475	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	11	45	theme	novel	1679:1683	arg1	regulator					1701:1709	a novel transcriptional regulator	1677:1709	a novel transcriptional regulator of HS	1677:1715	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	11	45	theme	novel	1679:1683	arg1	TFCP2					1668:1672	TFCP2	1668:1672	TFCP2	1668:1672	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	1	46	theme	long	117:120	arg1	polysaccharide					130:143	a long, linear polysaccharide	115:143	a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development	115:284	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	1	46	theme	long	117:120	arg1	sulfate					99:105	Heparan sulfate	91:105	Heparan sulfate (HS)	91:110	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	3	47	theme	endoplasmic	482:492	arg1	reticulum					494:502	the endoplasmic reticulum	478:502	the endoplasmic reticulum	478:502	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	10	48	theme	growth	1495:1500	arg1	factor					1502:1507	growth factor	1495:1507	growth factor binding	1495:1515	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	7	49	theme	human	1046:1050	arg1	cells					1061:1065	human melanoma cells	1046:1065	human melanoma cells	1046:1065	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	1	50	theme	key	208:210	arg1	role					212:215	a key role	206:215	a key role	206:215	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	10	51	from	expression	1572:1581	arg1	cells					1599:1603	TFCP2 mutant cells	1586:1603	TFCP2 mutant cells	1586:1603	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	2	52	theme	HS	304:305	arg1	assembly					307:314	HS assembly	304:314	HS assembly	304:314	Dysregulation of HS assembly has been implicated in pathophysiological conditions, such as tumorigenesis and rare genetic disorders.
37061003	9	53	theme	HS	1375:1376	arg1	assembly					1378:1385	HS assembly	1375:1385	HS assembly	1375:1385	Additionally, RNA sequencing revealed that TFCP2 regulates expression of multiple enzymes involved in HS assembly, including the secreted endosulfatase, SULF1.
37061003	8	54	theme	TFCP2	1097:1101	arg1	cells					1112:1116	TFCP2 knockout cells	1097:1116	TFCP2 knockout cells	1097:1116	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	8	55	theme	cell	1174:1177	arg1	HS					1187:1188	cell surface HS	1174:1188	cell surface HS	1174:1188	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	5	56	theme	regulatory	832:841	arg1	factors					843:849	novel regulatory factors	826:849	novel regulatory factors of HS biosynthesis	826:868	To address this gap in knowledge, we recently performed genome-wide CRISPR/Cas9 screens to identify novel regulatory factors of HS biosynthesis.
37061003	7	57	theme	HS	1001:1002	arg1	assembly					1004:1011	HS assembly	1001:1011	HS assembly	1001:1011	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	3	58	from	manner	468:473	arg1	Golgi					508:512	Golgi	508:512	Golgi	508:512	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	3	58	from	manner	468:473	arg1	reticulum					494:502	the endoplasmic reticulum	478:502	the endoplasmic reticulum	478:502	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	5	59	theme	genome-wide	782:792	arg1	screens					806:812	genome-wide CRISPR/Cas9 screens	782:812	genome-wide CRISPR/Cas9 screens	782:812	To address this gap in knowledge, we recently performed genome-wide CRISPR/Cas9 screens to identify novel regulatory factors of HS biosynthesis.
37061003	8	60	theme	decreased	1128:1136	arg1	binding					1163:1169	decreased fibroblast growth factor binding	1128:1169	decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells	1128:1270	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	6	61	theme	transcription	922:934	arg1	hit					960:962	a top hit	954:962	a top hit	954:962	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	6	61	theme	transcription	922:934	arg1	TFCP2					944:948	TFCP2	944:948	TFCP2	944:948	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	6	61	theme	transcription	922:934	arg1	factor					936:941	the alpha globin transcription factor	905:941	the alpha globin transcription factor	905:941	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	10	62	theme	TFCP2	1586:1590	arg1	cells					1599:1603	TFCP2 mutant cells	1586:1603	TFCP2 mutant cells	1586:1603	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	7	63	theme	TFCP2	992:996	arg1	role					984:987	the role	980:987	the role of TFCP2 in HS assembly	980:1011	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	3	64	theme	biosynthetic	555:566	arg1	enzymes					568:574	biosynthetic enzymes	555:574	biosynthetic enzymes	555:574	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	8	65	theme	growth	1149:1154	arg1	factor					1156:1161	fibroblast growth factor	1138:1161	decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells	1128:1270	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	6	66	theme	alpha	909:913	arg1	hit					960:962	a top hit	954:962	a top hit	954:962	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	6	66	theme	alpha	909:913	arg1	TFCP2					944:948	TFCP2	944:948	TFCP2	944:948	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	6	66	theme	alpha	909:913	arg1	factor					936:941	the alpha globin transcription factor	905:941	the alpha globin transcription factor	905:941	From these screens, we identified the alpha globin transcription factor, TFCP2, as a top hit.
37061003	8	67	theme	HS	1206:1207	arg1	composition					1209:1219	HS composition	1206:1219	HS composition	1206:1219	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	10	68	theme	expression	1572:1581	arg1	knockdown					1553:1561	knockdown	1553:1561	knockdown of SULF1 expression in TFCP2 mutant cells	1553:1603	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	3	69	theme	group	546:550	arg1	activity					526:533	the activity	522:533	the activity of a large group of biosynthetic enzymes	522:574	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	8	70	from	HS	1187:1188	arg1	composition					1209:1219	HS composition	1206:1219	HS composition	1206:1219	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	4	71	theme	HS	666:667	arg1	assembly					669:676	HS assembly	666:676	HS assembly	666:676	While much is known about its biosynthesis, little is understood about the regulation of HS assembly across diverse tissue types and disease states.
37061003	11	72	theme	slow	1779:1782	arg1	growth					1793:1798	slow melanoma growth	1779:1798	slow melanoma growth	1779:1798	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	8	73	theme	slowed	1226:1231	arg1	growth					1238:1243	slowed cell growth	1226:1243	slowed cell growth	1226:1243	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	11	74	theme	HS	1714:1715	arg1	regulator					1701:1709	a novel transcriptional regulator	1677:1709	a novel transcriptional regulator of HS	1677:1715	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	11	74	theme	HS	1714:1715	arg1	TFCP2					1668:1672	TFCP2	1668:1672	TFCP2	1668:1672	Overall, these studies identify TFCP2 as a novel transcriptional regulator of HS and highlight HS-protein interactions as a possible target to slow melanoma growth.
37061003	0	75	theme	cell	78:81	arg1	growth					83:88	melanoma cell growth	69:88	melanoma cell growth	69:88	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	8	76	from	alterations	1191:1201	arg1	composition					1209:1219	HS composition	1206:1219	HS composition	1206:1219	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	5	77	theme	biosynthesis	857:868	arg1	factors					843:849	novel regulatory factors	826:849	novel regulatory factors of HS biosynthesis	826:868	To address this gap in knowledge, we recently performed genome-wide CRISPR/Cas9 screens to identify novel regulatory factors of HS biosynthesis.
37061003	5	78	from	gap	742:744	arg1	knowledge					749:757	knowledge	749:757	knowledge	749:757	To address this gap in knowledge, we recently performed genome-wide CRISPR/Cas9 screens to identify novel regulatory factors of HS biosynthesis.
37061003	5	79	theme	CRISPR/Cas9	794:804	arg1	screens					806:812	genome-wide CRISPR/Cas9 screens	782:812	genome-wide CRISPR/Cas9 screens	782:812	To address this gap in knowledge, we recently performed genome-wide CRISPR/Cas9 screens to identify novel regulatory factors of HS biosynthesis.
37061003	3	80	dep	driven	461:466	arg1	non-template					448:459	non-template	448:459	non-template	448:459	HS biosynthesis occurs in a non-template driven manner in the endoplasmic reticulum and Golgi through the activity of a large group of biosynthetic enzymes.
37061003	4	81	theme	tissue	693:698	arg1	types					700:704	diverse tissue types	685:704	diverse tissue types	685:704	While much is known about its biosynthesis, little is understood about the regulation of HS assembly across diverse tissue types and disease states.
37061003	10	82	theme	mutant	1592:1597	arg1	cells					1599:1603	TFCP2 mutant cells	1586:1603	TFCP2 mutant cells	1586:1603	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	0	83	theme	transcriptional	11:25	arg1	TFCP2					0:4	TFCP2	0:4	TFCP2	0:4	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	0	83	theme	transcriptional	11:25	arg1	regulator					27:35	a transcriptional regulator	9:35	a transcriptional regulator of heparan sulfate assembly and melanoma cell growth	9:88	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	8	84	from	growth	1238:1243	arg1	composition					1209:1219	HS composition	1206:1219	HS composition	1206:1219	TFCP2 knockout cells exhibited decreased fibroblast growth factor binding to cell surface HS, alterations in HS composition, and slowed cell growth compared to wildtype cells.
37061003	1	85	theme	Heparan	91:97	arg1	polysaccharide					130:143	a long, linear polysaccharide	115:143	a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development	115:284	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	1	85	theme	Heparan	91:97	arg1	HS					108:109	HS	108:109	HS	108:109	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	1	85	theme	Heparan	91:97	arg1	sulfate					99:105	Heparan sulfate	91:105	Heparan sulfate (HS)	91:110	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	2	86	theme	rare	396:399	arg1	disorders					409:417	rare genetic disorders	396:417	rare genetic disorders	396:417	Dysregulation of HS assembly has been implicated in pathophysiological conditions, such as tumorigenesis and rare genetic disorders.
37061003	10	87	theme	Pharmacological	1433:1447	arg1	targeting					1449:1457	Pharmacological targeting	1433:1457	Pharmacological targeting of TFCP2 activity	1433:1475	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	1	88	dep	long	117:120	arg1	linear					123:128	linear	123:128	linear	123:128	Heparan sulfate (HS) is a long, linear polysaccharide that is ubiquitously expressed in all animal cells and plays a key role in many cellular processes, including cell signaling and development.
37061003	7	89	from	expression	1032:1041	arg1	cells					1061:1065	human melanoma cells	1046:1065	human melanoma cells	1046:1065	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	10	90	theme	cell	1623:1626	arg1	growth					1628:1633	melanoma cell growth	1614:1633	melanoma cell growth	1614:1633	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	0	91	theme	sulfate	48:54	arg1	assembly					56:63	heparan sulfate assembly	40:63	heparan sulfate assembly	40:63	TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth.
37061003	10	92	theme	activity	1468:1475	arg1	targeting					1449:1457	Pharmacological targeting	1433:1457	Pharmacological targeting of TFCP2 activity	1433:1475	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
37061003	7	93	from	role	984:987	arg1	assembly					1004:1011	HS assembly	1001:1011	HS assembly	1001:1011	To investigate the role of TFCP2 in HS assembly, we targeted TFCP2 expression in human melanoma cells using the CRISPR/Cas9 system.
37061003	10	94	theme	factor	1502:1507	arg1	binding					1509:1515	growth factor binding	1495:1515	growth factor binding	1495:1515	Pharmacological targeting of TFCP2 activity similarly reduced growth factor binding and increased SULF1 expression, and knockdown of SULF1 expression in TFCP2 mutant cells restored melanoma cell growth.
35647280	4	0	theme	metagenomic	903:913	arg1	libraries					915:923	metagenomic libraries	903:923	metagenomic libraries	903:923	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	7	1	theme	careful	1477:1483	arg1	consideration					1485:1497	careful consideration	1477:1497	careful consideration	1477:1497	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	5	2	theme	secondary	936:944	arg1	carbons					946:952	secondary carbons	936:952	secondary carbons	936:952	Azides at secondary carbons are not significantly accommodated, and thus, associated substrates are not processed, while those at primary carbons are productively recognized by only a small subset of the enzymes and often then only very poorly.
35647280	7	3	theme	such	1560:1563	arg1	studies					1565:1571	such studies	1560:1571	such studies	1560:1571	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	4	4	theme	N-acetylhexosaminides	804:824	arg1	series					768:773	a series	766:773	a series of azidodeoxy glucosides and N-acetylhexosaminides	766:824	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	6	5	theme	situation	1297:1305	arg1	representative					1275:1288	representative	1275:1288	representative	1275:1288	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	5	6	from	carbons	946:952	arg1	Azides					926:931	Azides	926:931	Azides at secondary carbons	926:952	Azides at secondary carbons are not significantly accommodated, and thus, associated substrates are not processed, while those at primary carbons are productively recognized by only a small subset of the enzymes and often then only very poorly.
35647280	4	7	theme	azidodeoxy	778:787	arg1	glucosides					789:798	azidodeoxy glucosides	778:798	azidodeoxy glucosides	778:798	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	3	8	theme	model	560:564	arg1	systems					573:579	tractable model enzyme systems	550:579	tractable model enzyme systems	550:579	To quantitatively assess how well azides are accommodated, we have used glycosidases as tractable model enzyme systems reflecting what would also be expected for glycosyltransferases and other sugar binding/modifying proteins.
35647280	3	9	theme	tractable	550:558	arg1	systems					573:579	tractable model enzyme systems	550:579	tractable model enzyme systems	550:579	To quantitatively assess how well azides are accommodated, we have used glycosidases as tractable model enzyme systems reflecting what would also be expected for glycosyltransferases and other sugar binding/modifying proteins.
35647280	5	10	theme	enzymes	1130:1136	arg1	subset					1116:1121	only a small subset	1103:1121	only a small subset of the enzymes	1103:1136	Azides at secondary carbons are not significantly accommodated, and thus, associated substrates are not processed, while those at primary carbons are productively recognized by only a small subset of the enzymes and often then only very poorly.
35647280	4	11	theme	expressed	874:882	arg1	gene					894:897	expressed synthetic gene	874:897	expressed synthetic gene	874:897	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	1	12	theme	Azido	74:78	arg1	sugars					80:85	Azido sugars	74:85	Azido sugars	74:85	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	7	13	used	used	1587:1590	arg2	studies					1565:1571	such studies	1560:1571	such studies	1560:1571	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	4	14	theme	glucosides	789:798	arg1	series					768:773	a series	766:773	a series of azidodeoxy glucosides and N-acetylhexosaminides	766:824	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	3	15	used	used	529:532	arg2	we					521:522	we	521:522	we	521:522	To quantitatively assess how well azides are accommodated, we have used glycosidases as tractable model enzyme systems reflecting what would also be expected for glycosyltransferases and other sugar binding/modifying proteins.
35647280	7	16	theme	glycosylation	1647:1659	arg1	activity					1661:1668	glycosylation activity	1647:1668	glycosylation activity	1647:1668	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	4	17	theme	series	768:773	arg1	hydrolysis					752:761	the hydrolysis	748:761	the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides	748:824	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	0	18	theme	Azido	0:4	arg1	Groups					6:11	Azido Groups	0:11	Azido Groups	0:11	Azido Groups Hamper Glycan Acceptance by Carbohydrate Processing Enzymes.
35647280	7	19	theme	activity	1661:1668	arg1	absence					1636:1642	the absence	1632:1642	the absence of glycosylation activity	1632:1668	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	4	20	theme	synthetic	884:892	arg1	gene					894:897	expressed synthetic gene	874:897	expressed synthetic gene	874:897	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	2	21	theme	azide-substituted	313:329	arg1	sugars					331:336	azide-substituted sugars	313:336	azide-substituted sugars	313:336	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	1	22	theme	metabolic	183:191	arg1	labeling					193:200	metabolic labeling	183:200	cell surface metabolic labeling	170:200	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	0	23	theme	Glycan	20:25	arg1	Acceptance					27:36	Glycan Acceptance	20:36	Glycan Acceptance	20:36	Azido Groups Hamper Glycan Acceptance by Carbohydrate Processing Enzymes.
35647280	6	24	theme	considerable	1361:1372	arg1	caution					1374:1380	considerable caution	1361:1380	considerable caution	1361:1380	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	6	25	dep	controls	1210:1217	arg1	absence					1191:1197	absence	1191:1197	absence	1191:1197	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	6	25	dep	controls	1210:1217	arg1	the					1187:1189	the	1187:1189	the	1187:1189	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	1	26	theme	frequent	98:105	arg1	use					107:109	frequent use	98:109	frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases	98:254	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	2	27	theme	azide	432:436	arg1	size					407:410	size	407:410	size	407:410	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	2	27	theme	azide	432:436	arg1	structure					416:424	structure	416:424	structure	416:424	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	6	28	theme	careful	1202:1208	arg1	controls					1210:1217	careful controls	1202:1217	careful controls	1202:1217	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	3	29	theme	binding/modifying	661:677	arg1	proteins					679:686	other sugar binding/modifying proteins	649:686	other sugar binding/modifying proteins	649:686	To quantitatively assess how well azides are accommodated, we have used glycosidases as tractable model enzyme systems reflecting what would also be expected for glycosyltransferases and other sugar binding/modifying proteins.
35647280	3	30	theme	enzyme	566:571	arg1	systems					573:579	tractable model enzyme systems	550:579	tractable model enzyme systems	550:579	To quantitatively assess how well azides are accommodated, we have used glycosidases as tractable model enzyme systems reflecting what would also be expected for glycosyltransferases and other sugar binding/modifying proteins.
35647280	1	31	theme	systems	135:141	arg1	probes					114:119	probes	114:119	probes of biological systems	114:141	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	5	32	theme	associated	1000:1009	arg1	substrates					1011:1020	associated substrates	1000:1020	associated substrates	1000:1020	Azides at secondary carbons are not significantly accommodated, and thus, associated substrates are not processed, while those at primary carbons are productively recognized by only a small subset of the enzymes and often then only very poorly.
35647280	5	33	theme	small	1110:1114	arg1	subset					1116:1121	only a small subset	1103:1121	only a small subset of the enzymes	1103:1136	Azides at secondary carbons are not significantly accommodated, and thus, associated substrates are not processed, while those at primary carbons are productively recognized by only a small subset of the enzymes and often then only very poorly.
35647280	1	34	dep	surface	175:181	arg1	labeling					193:200	metabolic labeling	183:200	cell surface metabolic labeling	170:200	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	1	35	theme	activity-based	205:218	arg1	profiling					230:238	activity-based proteomic profiling	205:238	activity-based proteomic profiling of glycosidases	205:254	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	4	36	theme	specificity	703:713	arg1	constants					715:723	specificity constants	703:723	specificity constants	703:723	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	7	37	theme	useful	1424:1429	arg1	tool					1431:1434	a useful tool	1422:1434	a useful tool to monitor and detect glycosylation	1422:1470	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	3	38	theme	other	649:653	arg1	proteins					679:686	other sugar binding/modifying proteins	649:686	other sugar binding/modifying proteins	649:686	To quantitatively assess how well azides are accommodated, we have used glycosidases as tractable model enzyme systems reflecting what would also be expected for glycosyltransferases and other sugar binding/modifying proteins.
35647280	5	39	theme	primary	1056:1062	arg1	carbons					1064:1070	primary carbons	1056:1070	primary carbons	1056:1070	Azides at secondary carbons are not significantly accommodated, and thus, associated substrates are not processed, while those at primary carbons are productively recognized by only a small subset of the enzymes and often then only very poorly.
35647280	1	40	theme	proteomic	220:228	arg1	profiling					230:238	activity-based proteomic profiling	205:238	activity-based proteomic profiling of glycosidases	205:254	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	0	41	theme	Processing	54:63	arg1	Enzymes					65:71	Carbohydrate Processing Enzymes	41:71	Carbohydrate Processing Enzymes	41:71	Azido Groups Hamper Glycan Acceptance by Carbohydrate Processing Enzymes.
35647280	3	42	theme	sugar	655:659	arg1	proteins					679:686	other sugar binding/modifying proteins	649:686	other sugar binding/modifying proteins	649:686	To quantitatively assess how well azides are accommodated, we have used glycosidases as tractable model enzyme systems reflecting what would also be expected for glycosyltransferases and other sugar binding/modifying proteins.
35647280	6	43	theme	azide-modified	1242:1255	arg1	sugars					1257:1262	azide-modified sugars	1242:1262	azide-modified sugars	1242:1262	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	2	44	from	difference	393:402	arg1	size					407:410	size	407:410	size	407:410	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	2	44	from	difference	393:402	arg1	structure					416:424	structure	416:424	structure	416:424	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	0	45	theme	Carbohydrate	41:52	arg1	Enzymes					65:71	Carbohydrate Processing Enzymes	41:71	Carbohydrate Processing Enzymes	41:71	Azido Groups Hamper Glycan Acceptance by Carbohydrate Processing Enzymes.
35647280	4	46	theme	glycosidases	847:858	arg1	number					837:842	a large number	829:842	a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries	829:923	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	2	47	theme	parent	352:357	arg1	molecule					359:366	the parent molecule	348:366	the parent molecule	348:366	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	6	48	with	situation	1297:1305	arg1	sugar					1324:1328	the natural sugar	1312:1328	the natural sugar	1312:1328	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	7	49	theme	azide	1540:1544	arg1	substitution					1546:1557	azide substitution	1540:1557	azide substitution	1540:1557	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	7	50	theme	Azide	1383:1387	arg1	incorporation					1389:1401	Azide incorporation	1383:1401	Azide incorporation	1383:1401	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	1	51	from	use	107:109	arg1	approaches					146:155	approaches	146:155	approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases	146:254	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	6	52	theme	natural	1316:1322	arg1	sugar					1324:1328	the natural sugar	1312:1328	the natural sugar	1312:1328	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	2	53	theme	little	266:271	arg1	attention					273:281	little attention	266:281	little attention	266:281	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	1	54	theme	biological	124:133	arg1	systems					135:141	biological systems	124:141	biological systems	124:141	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	7	55	theme	sites	1531:1535	arg1	selection					1518:1526	the selection	1514:1526	the selection of sites of azide substitution	1514:1557	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	4	56	theme	large	831:835	arg1	number					837:842	a large number	829:842	a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries	829:923	In this vein, specificity constants have been measured for the hydrolysis of a series of azidodeoxy glucosides and N-acetylhexosaminides by a large number of glycosidases produced from expressed synthetic gene and metagenomic libraries.
35647280	2	57	theme	substantial	381:391	arg1	difference					393:402	the substantial difference	377:402	the substantial difference in size and structure of an azide compared to a hydroxyl	377:459	However, little attention is typically paid to how well azide-substituted sugars represent the parent molecule, despite the substantial difference in size and structure of an azide compared to a hydroxyl.
35647280	1	58	theme	cell	170:173	arg1	surface					175:181	cell surface metabolic labeling	170:200	cell surface metabolic labeling	170:200	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	1	59	theme	glycosidases	243:254	arg1	profiling					230:238	activity-based proteomic profiling	205:238	activity-based proteomic profiling of glycosidases	205:254	Azido sugars have found frequent use as probes of biological systems in approaches ranging from cell surface metabolic labeling to activity-based proteomic profiling of glycosidases.
35647280	6	60	theme	controls	1210:1217	arg1	representative					1275:1288	representative	1275:1288	representative	1275:1288	Accordingly, in the absence of careful controls, results obtained with azide-modified sugars may not be representative of the situation with the natural sugar and should be interpreted with considerable caution.
35647280	7	61	theme	substitution	1546:1557	arg1	sites					1531:1535	sites	1531:1535	sites of azide substitution	1531:1557	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35647280	7	61	theme	substitution	1546:1557	arg1	substitution					1546:1557	azide substitution	1540:1557	azide substitution	1540:1557	Azide incorporation can indeed provide a useful tool to monitor and detect glycosylation, but careful consideration should go into the selection of sites of azide substitution; such studies should not be used to quantitate glycosylation or to infer the absence of glycosylation activity.
35357206	4	0	theme	voided	806:811	arg1	urine					827:831	the first voided early morning urine	796:831	the first voided early morning urine (morning urine)	796:847	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	0	theme	voided	806:811	arg1	urine					842:846	morning urine	834:846	morning urine	834:846	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	6	1	theme	urine	1181:1185	arg1	samples					1187:1193	Paired urine samples	1174:1193	Paired urine samples of spot and morning urine	1174:1219	Paired urine samples of spot and morning urine were available for 389 patients.
35357206	12	2	theme	FujiLAM	1917:1923	arg1	sensitivity					1925:1935	FujiLAM sensitivity	1917:1935	FujiLAM sensitivity	1917:1935	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	12	3	dep	93.1	2073:2076	arg1	to					2070:2071	to	2070:2071	to	2070:2071	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	1	4	theme	TB	243:244	arg1	AlereLAM					254:261	AlereLAM	254:261	AlereLAM	254:261	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	1	4	theme	TB	243:244	arg1	Ag					250:251	TB LAM Ag	243:251	TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV	243:316	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	4	5	theme	morning	819:825	arg1	urine					827:831	the first voided early morning urine	796:831	the first voided early morning urine (morning urine)	796:847	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	5	theme	morning	819:825	arg1	urine					842:846	morning urine	834:846	morning urine	834:846	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	10	6	theme	%	1579:1579	arg1	sensitivity					1564:1574	the FujiLAM sensitivity	1552:1574	the FujiLAM sensitivity (95% confidence interval [CI])	1552:1605	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	6	theme	%	1579:1579	arg1	[CI					1601:1603	95% confidence interval [CI	1577:1603	95% confidence interval [CI	1577:1603	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	7	7	theme	interquartile	1308:1320	arg1	range					1322:1326	interquartile range	1308:1326	interquartile range [IQR	1308:1331	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	3	8	theme	diagnostic	480:489	arg1	accuracy					491:498	the diagnostic accuracy	476:498	the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	476:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	16	9	theme	early	2493:2497	arg1	urine					2507:2511	spot and early morning urine	2484:2511	urine	2507:2511	IMPORTANCE This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV.
35357206	7	10	theme	cells/μL	1298:1305	arg1	count					1285:1289	a median CD4 cell count	1267:1289	a median CD4 cell count of 176 cells/μL	1267:1305	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	11	11	theme	%	1868:1868	arg1	difference					1851:1860	a difference	1849:1860	a difference of 1.2%	1849:1868	Specificity was 90.2% (86.5 to 93.1) versus 89.0% (85.2 to 92.1) for spot and morning urine, respectively, a difference of 1.2% (-3.7 to 1.4).
35357206	10	12	theme	microbiological	1516:1530	arg1	reference					1532:1540	a microbiological reference	1514:1540	a microbiological reference standard	1514:1549	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	16	13	theme	urine	2507:2511	arg1	samples					2513:2519	spot and early morning urine samples	2484:2519	spot and early morning urine samples	2484:2519	IMPORTANCE This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV.
35357206	1	14	theme	TB	170:171	arg1	assay					187:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	11	15	dep	%	1762:1762	arg1	1.4					1879:1881	1.4	1879:1881	1.4	1879:1881	Specificity was 90.2% (86.5 to 93.1) versus 89.0% (85.2 to 92.1) for spot and morning urine, respectively, a difference of 1.2% (-3.7 to 1.4).
35357206	14	16	theme	lower	2324:2328	arg1	specificity					2330:2340	lower specificity	2324:2340	lower specificity	2324:2340	Sensitivity could be increased with a two-sample strategy but at the risk of lower specificity.
35357206	7	17	theme	cell	1280:1283	arg1	count					1285:1289	a median CD4 cell count	1267:1289	a median CD4 cell count of 176 cells/μL	1267:1305	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	9	18	dep	spot	1438:1441	arg1	results					1469:1475	test results	1464:1475	test results	1464:1475	Overall agreement for spot versus morning urine test results was 94.6% (kappa, 0.81).
35357206	17	19	theme	lower	2648:2652	arg1	specificity					2654:2664	lower specificity	2648:2664	lower specificity	2648:2664	Sensitivity could be increased with a two-sample strategy but at the risk of lower specificity.
35357206	0	20	theme	Tuberculosis	113:124	arg1	Diagnosis					100:108	Diagnosis	100:108	Diagnosis of Tuberculosis among People with HIV	100:146	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	12	21	dep	90.6	2097:2100	arg1	to					2094:2095	to	2094:2095	to	2094:2095	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	9	22	theme	0.81	1495:1498	arg1	kappa					1488:1492	kappa	1488:1492	kappa	1488:1492	Overall agreement for spot versus morning urine test results was 94.6% (kappa, 0.81).
35357206	7	23	theme	median	1269:1274	arg1	count					1285:1289	a median CD4 cell count	1267:1289	a median CD4 cell count of 176 cells/μL	1267:1305	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	11	24	dep	93.1	1773:1776	arg1	to					1770:1771	to	1770:1771	to	1770:1771	Specificity was 90.2% (86.5 to 93.1) versus 89.0% (85.2 to 92.1) for spot and morning urine, respectively, a difference of 1.2% (-3.7 to 1.4).
35357206	7	25	contain	had	1263:1265	arg1	Patients					1254:1261	Patients	1254:1261	Patients	1254:1261	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	7	25	contain	had	1263:1265	arg2	count					1285:1289	a median CD4 cell count	1267:1289	a median CD4 cell count of 176 cells/μL	1267:1305	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	5	26	theme	microbiological	978:992	arg1	standards					1101:1109	both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards	973:1109	both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases)	973:1171	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	5	26	theme	microbiological	978:992	arg1	cases					1166:1170	microbiologically confirmed and probable TB cases	1122:1170	microbiologically confirmed and probable TB cases	1122:1170	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	10	27	theme	confidence	1581:1590	arg1	sensitivity					1564:1574	the FujiLAM sensitivity	1552:1574	the FujiLAM sensitivity (95% confidence interval [CI])	1552:1605	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	27	theme	confidence	1581:1590	arg1	[CI					1601:1603	95% confidence interval [CI	1577:1603	95% confidence interval [CI	1577:1603	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	2	28	from	value	432:436	arg1	urine					394:398	early morning urine	380:398	early morning urine	380:398	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	2	28	from	value	432:436	arg1	urine					412:416	spot urine	407:416	spot urine	407:416	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	8	29	contain	had	1399:1401	arg1	19					1389:1390	19	1389:1390	19	1389:1390	Forty-three (11.0%) had confirmed TB, and 19 (4.9%) had probable TB.
35357206	8	29	contain	had	1399:1401	arg1	%					1396:1396	4.9%	1393:1396	4.9%	1393:1396	Forty-three (11.0%) had confirmed TB, and 19 (4.9%) had probable TB.
35357206	8	29	contain	had	1399:1401	arg2	TB					1412:1413	probable TB	1403:1413	probable TB	1403:1413	Forty-three (11.0%) had confirmed TB, and 19 (4.9%) had probable TB.
35357206	3	30	from	treatment	644:652	arg1	Ghana					657:661	Ghana	657:661	Ghana	657:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	5	31	theme	Diagnostic	930:939	arg1	accuracy					941:948	Diagnostic accuracy	930:948	Diagnostic accuracy	930:948	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	3	32	theme	urine	528:532	arg1	samples					534:540	cryopreserved urine samples	514:540	cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	514:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	9	33	theme	test	1464:1467	arg1	results					1469:1475	test results	1464:1475	test results	1464:1475	Overall agreement for spot versus morning urine test results was 94.6% (kappa, 0.81).
35357206	0	34	with	People	132:137	arg1	HIV					144:146	HIV	144:146	HIV	144:146	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	13	35	theme	urine	2232:2236	arg1	samples					2238:2244	spot and early morning urine samples	2209:2244	spot and early morning urine samples	2209:2244	This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples.
35357206	7	36	dep	range	1322:1326	arg1	361					1341:1343	361	1341:1343	361	1341:1343	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	7	36	dep	range	1322:1326	arg1	[IQR					1328:1331	[IQR	1328:1331	[IQR	1328:1331	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	11	37	theme	morning	1820:1826	arg1	urine					1828:1832	morning urine	1820:1832	morning urine	1820:1832	Specificity was 90.2% (86.5 to 93.1) versus 89.0% (85.2 to 92.1) for spot and morning urine, respectively, a difference of 1.2% (-3.7 to 1.4).
35357206	0	38	theme	Morning	64:70	arg1	Urine					72:76	Early Morning Urine	58:76	Early Morning Urine	58:76	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	5	39	theme	Xpert	1020:1024	arg1	testing					1034:1040	Xpert MTB/RIF testing	1020:1040	Xpert MTB/RIF testing	1020:1040	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	1	40	with	people	302:307	arg1	HIV					314:316	HIV	314:316	HIV	314:316	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	2	41	theme	added	426:430	arg1	value					432:436	the added value	422:436	the added value of a two-sample strategy	422:461	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	4	42	theme	morning	834:840	arg1	urine					827:831	the first voided early morning urine	796:831	the first voided early morning urine (morning urine)	796:847	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	42	theme	morning	834:840	arg1	urine					842:846	morning urine	834:846	morning urine	834:846	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	5	43	theme	TB	1163:1164	arg1	cases					1166:1170	microbiologically confirmed and probable TB cases	1122:1170	microbiologically confirmed and probable TB cases	1122:1170	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	10	44	theme	%	1723:1723	arg1	%					1711:1711	95% CI	1709:1714	95% CI	1709:1714	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	%					1615:1615	67.4%	1611:1615	67.4% (51.5 to 80.9) for spot	1611:1639	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	82.8					1660:1663	82.8	1660:1663	82.8	1660:1663	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	14.8					1735:1738	14.8	1735:1738	14.8	1735:1738	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	sensitivity					1564:1574	the FujiLAM sensitivity	1552:1574	the FujiLAM sensitivity (95% confidence interval [CI])	1552:1605	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	difference					1697:1706	an absolute difference	1685:1706	an absolute difference (95% CI) of 2.4% (-10.2 to 14.8)	1685:1739	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	%					1649:1649	69.8%	1645:1649	69.8% (53.9 to 82.8) for morning urine	1645:1682	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	%					1723:1723	2.4%	1720:1723	2.4% (-10.2 to 14.8)	1720:1739	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	44	theme	%	1723:1723	arg1	80.9					1626:1629	80.9	1626:1629	80.9	1626:1629	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	16	45	theme	FujiLAM	2443:2449	arg1	testing					2451:2457	FujiLAM testing	2443:2457	FujiLAM testing	2443:2457	IMPORTANCE This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV.
35357206	13	46	theme	spot	2209:2212	arg1	urine					2232:2236	spot and early morning urine	2209:2236	urine	2232:2236	This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples.
35357206	1	47	theme	Fujifilm	153:160	arg1	assay					187:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	10	48	theme	standard	1542:1549	arg1	reference					1532:1540	a microbiological reference	1514:1540	a microbiological reference standard	1514:1549	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	12	49	dep	16.9	2018:2021	arg1	to					2015:2016	to	2015:2016	to	2015:2016	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	2	50	theme	two-sample	443:452	arg1	strategy					454:461	a two-sample strategy	441:461	a two-sample strategy	441:461	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	4	51	theme	voided	729:734	arg1	urine					736:740	voided urine	729:740	spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine)	715:847	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	2	52	theme	diagnostic	341:350	arg1	value					352:356	the diagnostic value	337:356	the diagnostic value of FujiLAM testing on early morning urine versus spot urine	337:416	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	9	53	dep	%	1485:1485	arg1	kappa					1488:1492	kappa	1488:1492	kappa	1488:1492	Overall agreement for spot versus morning urine test results was 94.6% (kappa, 0.81).
35357206	4	54	theme	FujiLAM	676:682	arg1	sensitivity					684:694	FujiLAM sensitivity	676:694	FujiLAM sensitivity	676:694	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	0	55	theme	Diagnostic	0:9	arg1	Accuracy					11:18	Diagnostic Accuracy	0:18	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.	0:147	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	2	56	theme	early	380:384	arg1	urine					394:398	early morning urine	380:398	early morning urine	380:398	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	2	57	theme	testing	369:375	arg1	value					432:436	the added value	422:436	the added value of a two-sample strategy	422:461	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	2	57	theme	testing	369:375	arg1	value					352:356	the diagnostic value	337:356	the diagnostic value of FujiLAM testing on early morning urine versus spot urine	337:416	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	12	58	dep	-0.8	2135:2138	arg1	to					2132:2133	to	2132:2133	to	2132:2133	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	4	59	theme	spot	896:899	arg1	urine					913:917	spot and morning urine	896:917	urine	913:917	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	59	theme	spot	896:899	arg1	samples					887:893	two samples	883:893	two samples (spot and morning urine)	883:918	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	3	60	theme	cohort	588:593	arg1	part					566:569	part	566:569	part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	566:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	0	61	theme	Lipoarabinomannan	29:45	arg1	Testing					47:53	Urine Lipoarabinomannan Testing	23:53	Urine Lipoarabinomannan Testing	23:53	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	5	62	theme	sputum	1045:1050	arg1	culture					1008:1014	sputum culture	1001:1014	sputum culture	1001:1014	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	5	62	theme	sputum	1045:1050	arg1	testing					1034:1040	Xpert MTB/RIF testing	1020:1040	Xpert MTB/RIF testing	1020:1040	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	4	63	theme	morning	905:911	arg1	urine					913:917	spot and morning urine	896:917	urine	913:917	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	63	theme	morning	905:911	arg1	samples					887:893	two samples	883:893	two samples (spot and morning urine)	883:918	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	5	64	theme	urine	1056:1060	arg1	culture					1008:1014	sputum culture	1001:1014	sputum culture	1001:1014	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	5	64	theme	urine	1056:1060	arg1	testing					1034:1040	Xpert MTB/RIF testing	1020:1040	Xpert MTB/RIF testing	1020:1040	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	9	65	theme	Overall	1416:1422	arg1	agreement					1424:1432	Overall agreement	1416:1432	Overall agreement for spot versus morning urine test results	1416:1475	Overall agreement for spot versus morning urine test results was 94.6% (kappa, 0.81).
35357206	1	66	theme	improved	200:207	arg1	sensitivity					209:219	improved sensitivity	200:219	improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV	200:316	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	3	67	dep	samples	534:540	arg1	stored					556:561	stored	556:561	stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	556:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	3	67	dep	samples	534:540	arg1	collected					542:550	collected	542:550	collected	542:550	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	8	68	theme	probable	1403:1410	arg1	TB					1412:1413	probable TB	1403:1413	probable TB	1403:1413	Forty-three (11.0%) had confirmed TB, and 19 (4.9%) had probable TB.
35357206	3	69	theme	adults	598:603	arg1	cohort					588:593	a prospective cohort	574:593	a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	574:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	13	70	theme	FujiLAM	2168:2174	arg1	testing					2176:2182	FujiLAM testing	2168:2182	FujiLAM testing	2168:2182	This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples.
35357206	5	71	theme	confirmed	1140:1148	arg1	cases					1166:1170	microbiologically confirmed and probable TB cases	1122:1170	microbiologically confirmed and probable TB cases	1122:1170	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	2	72	theme	spot	407:410	arg1	urine					412:416	spot urine	407:416	spot urine	407:416	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	11	73	dep	1.4	1879:1881	arg1	to					1876:1877	to	1876:1877	to	1876:1877	Specificity was 90.2% (86.5 to 93.1) versus 89.0% (85.2 to 92.1) for spot and morning urine, respectively, a difference of 1.2% (-3.7 to 1.4).
35357206	5	74	theme	composite	1081:1089	arg1	standards					1101:1109	both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards	973:1109	both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases)	973:1171	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	5	74	theme	composite	1081:1089	arg1	cases					1166:1170	microbiologically confirmed and probable TB cases	1122:1170	microbiologically confirmed and probable TB cases	1122:1170	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	4	75	theme	first	800:804	arg1	urine					827:831	the first voided early morning urine	796:831	the first voided early morning urine (morning urine)	796:847	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	75	theme	first	800:804	arg1	urine					842:846	morning urine	834:846	morning urine	834:846	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	6	76	theme	Paired	1174:1179	arg1	samples					1187:1193	Paired urine samples	1174:1193	Paired urine samples of spot and morning urine	1174:1219	Paired urine samples of spot and morning urine were available for 389 patients.
35357206	9	77	theme	morning	1450:1456	arg1	urine					1458:1462	morning urine	1450:1462	morning urine	1450:1462	Overall agreement for spot versus morning urine test results was 94.6% (kappa, 0.81).
35357206	1	78	theme	LAM	246:248	arg1	AlereLAM					254:261	AlereLAM	254:261	AlereLAM	254:261	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	1	78	theme	LAM	246:248	arg1	Ag					250:251	TB LAM Ag	243:251	TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV	243:316	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	4	79	theme	early	813:817	arg1	urine					827:831	the first voided early morning urine	796:831	the first voided early morning urine (morning urine)	796:847	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	79	theme	early	813:817	arg1	urine					842:846	morning urine	834:846	morning urine	834:846	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	12	80	theme	%	2007:2007	arg1	difference					1990:1999	a difference	1988:1999	a difference of 7.0% (-3.0 to 16.9)	1988:2022	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	12	80	theme	%	2007:2007	arg1	%					1946:1946	67.4%	1942:1946	67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5)	1942:1985	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	3	81	with	adults	598:603	arg1	HIV					610:612	HIV	610:612	HIV presenting for antiretroviral treatment in Ghana	610:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	1	82	theme	SILVAMP	162:168	arg1	assay					187:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	14	83	theme	specificity	2330:2340	arg1	risk					2316:2319	the risk	2312:2319	the risk of lower specificity	2312:2340	Sensitivity could be increased with a two-sample strategy but at the risk of lower specificity.
35357206	3	84	theme	FujiLAM	503:509	arg1	accuracy					491:498	the diagnostic accuracy	476:498	the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	476:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	16	85	theme	morning	2499:2505	arg1	urine					2507:2511	spot and early morning urine	2484:2511	urine	2507:2511	IMPORTANCE This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV.
35357206	1	86	theme	LAM	173:175	arg1	assay					187:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	10	87	dep	14.8	1735:1738	arg1	to					1732:1733	to	1732:1733	to	1732:1733	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	16	88	dep	IMPORTANCE	2406:2415	arg1	indicates					2428:2436	indicates	2428:2436	indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV	2428:2568	IMPORTANCE This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV.
35357206	10	89	theme	morning	1670:1676	arg1	urine					1678:1682	morning urine	1670:1682	morning urine	1670:1682	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	90	theme	FujiLAM	1556:1562	arg1	sensitivity					1564:1574	the FujiLAM sensitivity	1552:1574	the FujiLAM sensitivity (95% confidence interval [CI])	1552:1605	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	90	theme	FujiLAM	1556:1562	arg1	%					1615:1615	67.4%	1611:1615	67.4% (51.5 to 80.9) for spot	1611:1639	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	90	theme	FujiLAM	1556:1562	arg1	[CI					1601:1603	95% confidence interval [CI	1577:1603	95% confidence interval [CI	1577:1603	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	90	theme	FujiLAM	1556:1562	arg1	%					1723:1723	2.4%	1720:1723	2.4% (-10.2 to 14.8)	1720:1739	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	1	91	theme	FujiLAM	178:184	arg1	assay					187:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay	149:191	The Fujifilm SILVAMP TB LAM (FujiLAM) assay offers improved sensitivity compared to Determine TB LAM Ag (AlereLAM) for detecting tuberculosis (TB) among people with HIV.
35357206	12	92	theme	%	2124:2124	arg1	difference					2106:2115	a difference	2104:2115	a difference of -2.9% (-4.9 to -0.8)	2104:2139	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	12	92	theme	%	2124:2124	arg1	%					2062:2062	90.2%	2058:2062	90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6)	2058:2101	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	17	93	theme	specificity	2654:2664	arg1	risk					2640:2643	the risk	2636:2643	the risk of lower specificity	2636:2664	Sensitivity could be increased with a two-sample strategy but at the risk of lower specificity.
35357206	12	94	dep	%	1946:1946	arg1	%					1970:1970	74.4%	1966:1970	67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5)	1942:1985	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	12	94	dep	%	1946:1946	arg1	to					1963:1964	to	1963:1964	to	1963:1964	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	10	95	theme	interval	1592:1599	arg1	sensitivity					1564:1574	the FujiLAM sensitivity	1552:1574	the FujiLAM sensitivity (95% confidence interval [CI])	1552:1605	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	95	theme	interval	1592:1599	arg1	[CI					1601:1603	95% confidence interval [CI	1577:1603	95% confidence interval [CI	1577:1603	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	7	96	theme	CD4	1276:1278	arg1	count					1285:1289	a median CD4 cell count	1267:1289	a median CD4 cell count of 176 cells/μL	1267:1305	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	10	97	theme	absolute	1688:1695	arg1	%					1711:1711	95% CI	1709:1714	95% CI	1709:1714	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	10	97	theme	absolute	1688:1695	arg1	difference					1697:1706	an absolute difference	1685:1706	an absolute difference (95% CI) of 2.4% (-10.2 to 14.8)	1685:1739	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	6	98	theme	morning	1207:1213	arg1	urine					1215:1219	morning urine	1207:1219	morning urine	1207:1219	Paired urine samples of spot and morning urine were available for 389 patients.
35357206	3	99	theme	cryopreserved	514:526	arg1	samples					534:540	cryopreserved urine samples	514:540	cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	514:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	3	100	from	accuracy	491:498	arg1	samples					534:540	cryopreserved urine samples	514:540	cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	514:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	6	101	theme	spot	1198:1201	arg1	samples					1187:1193	Paired urine samples	1174:1193	Paired urine samples of spot and morning urine	1174:1219	Paired urine samples of spot and morning urine were available for 389 patients.
35357206	12	102	dep	80.9	1957:1960	arg1	to					1954:1955	to	1954:1955	to	1954:1955	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	7	103	dep	had	1263:1265	arg1	range					1322:1326	interquartile range	1308:1326	interquartile range [IQR	1308:1331	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	12	104	theme	two-sample	1887:1896	arg1	strategy					1898:1905	A two-sample strategy	1885:1905	A two-sample strategy	1885:1905	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	10	105	dep	80.9	1626:1629	arg1	to					1623:1624	to	1623:1624	to	1623:1624	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	5	106	dep	microbiological	978:992	arg1	using					995:999	using	995:999	using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB	995:1074	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	5	107	theme	probable	1154:1161	arg1	cases					1166:1170	microbiologically confirmed and probable TB cases	1122:1170	microbiologically confirmed and probable TB cases	1122:1170	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	17	108	theme	two-sample	2609:2618	arg1	strategy					2620:2627	a two-sample strategy	2607:2627	a two-sample strategy	2607:2627	Sensitivity could be increased with a two-sample strategy but at the risk of lower specificity.
35357206	0	109	theme	Spot	85:88	arg1	Urine					90:94	Spot Urine	85:94	Spot Urine	85:94	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	5	110	theme	MTB/RIF	1026:1032	arg1	testing					1034:1040	Xpert MTB/RIF testing	1020:1040	Xpert MTB/RIF testing	1020:1040	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	0	111	from	Accuracy	11:18	arg1	Urine					72:76	Early Morning Urine	58:76	Early Morning Urine	58:76	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	0	111	from	Accuracy	11:18	arg1	Urine					90:94	Spot Urine	85:94	Spot Urine	85:94	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	13	112	theme	early	2218:2222	arg1	urine					2232:2236	spot and early morning urine	2209:2236	urine	2232:2236	This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples.
35357206	2	113	theme	strategy	454:461	arg1	value					432:436	the added value	422:436	the added value of a two-sample strategy	422:461	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	2	113	theme	strategy	454:461	arg1	value					352:356	the diagnostic value	337:356	the diagnostic value of FujiLAM testing on early morning urine versus spot urine	337:416	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	18	114	theme	future	2689:2694	arg1	guidelines					2696:2705	future guidelines	2689:2705	future guidelines	2689:2705	These data can inform future guidelines and clinical practice around FujiLAM.
35357206	18	115	theme	clinical	2711:2718	arg1	practice					2720:2727	clinical practice	2711:2727	clinical practice	2711:2727	These data can inform future guidelines and clinical practice around FujiLAM.
35357206	16	116	theme	spot	2484:2487	arg1	urine					2507:2511	spot and early morning urine	2484:2511	urine	2507:2511	IMPORTANCE This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV.
35357206	5	117	theme	sputum	1001:1006	arg1	culture					1008:1014	sputum culture	1001:1014	sputum culture	1001:1014	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	13	118	theme	morning	2224:2230	arg1	urine					2232:2236	spot and early morning urine	2209:2236	urine	2232:2236	This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples.
35357206	4	119	theme	spot	864:867	arg1	one					859:861	a one	857:861	a one (spot urine)	857:874	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	119	theme	spot	864:867	arg1	urine					869:873	spot urine	864:873	spot urine	864:873	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	11	120	dep	92.1	1801:1804	arg1	to					1798:1799	to	1798:1799	to	1798:1799	Specificity was 90.2% (86.5 to 93.1) versus 89.0% (85.2 to 92.1) for spot and morning urine, respectively, a difference of 1.2% (-3.7 to 1.4).
35357206	12	121	dep	86.5	1981:1984	arg1	to					1978:1979	to	1978:1979	to	1978:1979	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	2	122	theme	morning	386:392	arg1	urine					394:398	early morning urine	380:398	early morning urine	380:398	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	0	123	theme	Urine	23:27	arg1	Testing					47:53	Urine Lipoarabinomannan Testing	23:53	Urine Lipoarabinomannan Testing	23:53	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	7	124	dep	361	1341:1343	arg1	to					1338:1339	to	1338:1339	to	1338:1339	Patients had a median CD4 cell count of 176 cells/μL (interquartile range [IQR], 52 to 361).
35357206	15	125	theme	clinical	2387:2394	arg1	practice					2396:2403	clinical practice	2387:2403	clinical practice	2387:2403	These data can inform future guidelines and clinical practice.
35357206	10	126	dep	82.8	1660:1663	arg1	to					1657:1658	to	1657:1658	to	1657:1658	Compared to a microbiological reference standard, the FujiLAM sensitivity (95% confidence interval [CI]) was 67.4% (51.5 to 80.9) for spot and 69.8% (53.9 to 82.8) for morning urine, an absolute difference (95% CI) of 2.4% (-10.2 to 14.8).
35357206	3	127	theme	prospective	576:586	arg1	cohort					588:593	a prospective cohort	574:593	a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana	574:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35357206	2	128	theme	FujiLAM	361:367	arg1	testing					369:375	FujiLAM testing	361:375	FujiLAM testing	361:375	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	0	129	theme	Testing	47:53	arg1	Accuracy					11:18	Diagnostic Accuracy	0:18	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.	0:147	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	5	130	theme	reference	1091:1099	arg1	standards					1101:1109	both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards	973:1109	both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases)	973:1171	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	5	130	theme	reference	1091:1099	arg1	cases					1166:1170	microbiologically confirmed and probable TB cases	1122:1170	microbiologically confirmed and probable TB cases	1122:1170	Diagnostic accuracy was determined against both microbiological (using sputum culture and Xpert MTB/RIF testing of sputum and urine to confirm TB) and composite reference standards (including microbiologically confirmed and probable TB cases).
35357206	0	131	theme	Early	58:62	arg1	Urine					72:76	Early Morning Urine	58:76	Early Morning Urine	58:76	Diagnostic Accuracy of Urine Lipoarabinomannan Testing in Early Morning Urine versus Spot Urine for Diagnosis of Tuberculosis among People with HIV.
35357206	4	132	dep	one	859:861	arg1	strategy					920:927	strategy	920:927	strategy	920:927	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	133	theme	spot	774:777	arg1	inclusion					763:771	inclusion	763:771	inclusion (spot urine)	763:784	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	4	133	theme	spot	774:777	arg1	urine					779:783	spot urine	774:783	spot urine	774:783	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	15	134	theme	future	2365:2370	arg1	guidelines					2372:2381	future guidelines	2365:2381	future guidelines	2365:2381	These data can inform future guidelines and clinical practice.
35357206	16	135	with	people	2554:2559	arg1	HIV					2566:2568	HIV	2566:2568	HIV	2566:2568	IMPORTANCE This study indicates that FujiLAM testing performs equivalently on spot and early morning urine samples for detecting tuberculosis among people with HIV.
35357206	14	136	theme	two-sample	2285:2294	arg1	strategy					2296:2303	a two-sample strategy	2283:2303	a two-sample strategy	2283:2303	Sensitivity could be increased with a two-sample strategy but at the risk of lower specificity.
35357206	6	137	theme	urine	1215:1219	arg1	samples					1187:1193	Paired urine samples	1174:1193	Paired urine samples of spot and morning urine	1174:1219	Paired urine samples of spot and morning urine were available for 389 patients.
35357206	4	138	theme	urine	736:740	arg1	samples					742:748	spontaneously voided urine samples	715:748	spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine)	715:847	We compared FujiLAM sensitivity and specificity in spontaneously voided urine samples collected at inclusion (spot urine) versus in the first voided early morning urine (morning urine) and for a one (spot urine) versus two samples (spot and morning urine) strategy.
35357206	12	139	dep	%	2062:2062	arg1	%					2086:2086	87.3%	2082:2086	90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6)	2058:2101	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	12	139	dep	%	2062:2062	arg1	to					2079:2080	to	2079:2080	to	2079:2080	A two-sample strategy increased FujiLAM sensitivity from 67.4% (51.5 to 80.9) to 74.4% (58.8 to 86.5), a difference of 7.0% (-3.0 to 16.9), while specificity decreased from 90.2% (86.5 to 93.1) to 87.3% (83.3 to 90.6), a difference of -2.9% (-4.9 to -0.8).
35357206	2	140	from	value	352:356	arg1	urine					394:398	early morning urine	380:398	early morning urine	380:398	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	2	140	from	value	352:356	arg1	urine					412:416	spot urine	407:416	spot urine	407:416	Here, we examined the diagnostic value of FujiLAM testing on early morning urine versus spot urine and the added value of a two-sample strategy.
35357206	3	141	theme	antiretroviral	629:642	arg1	treatment					644:652	antiretroviral treatment	629:652	antiretroviral treatment in Ghana	629:661	We assessed the diagnostic accuracy of FujiLAM on cryopreserved urine samples collected and stored as part of a prospective cohort of adults with HIV presenting for antiretroviral treatment in Ghana.
35904257	4	0	theme	scanning	875:882	arg1	microscopy					893:902	scanning electron microscopy	875:902	scanning electron microscopy	875:902	The resulting scaffolds exhibit a highly porous microstructure and good conductivity, determined by scanning electron microscopy and electrochemical impedance spectroscopy, respectively.
35904257	8	1	dep	biomimetic	1480:1489	arg1	tissue-like					1515:1525	tissue-like	1515:1525	tissue-like	1515:1525	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	8	1	dep	biomimetic	1480:1489	arg1	in					1506:1507	in	1506:1507	in	1506:1507	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	7	2	theme	differentiated	1273:1286	arg1	cells					1295:1299	differentiated SHSY5Y cells	1273:1299	differentiated SHSY5Y cells	1273:1299	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds demonstrate that ECM constituents promote neuronal differentiation in situ.
35904257	2	3	theme	cell	384:387	arg1	survival					389:396	cell survival	384:396	cell survival	384:396	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	8	4	theme	biomimetic	1480:1489	arg1	platforms					1527:1535	engineered highly biomimetic and functional in vitro tissue-like platforms	1462:1535	engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling	1462:1564	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	1	5	theme	milieu	262:267	arg1	interactions					234:245	the cell-cell and cell-microenvironment interactions	194:245	the cell-cell and cell-microenvironment interactions of the in vivo milieu	194:267	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	5	6	theme	ideal	1053:1057	arg1	candidates					1059:1068	ideal candidates	1053:1068	ideal candidates for cell infiltration	1053:1090	These supports boast excellent mechanical stability and water uptake capacity, making them ideal candidates for cell infiltration.
35904257	3	7	theme	porous	535:540	arg1	scaffolds					555:563	porous 3D composite scaffolds	535:563	porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin)	535:730	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	6	8	theme	ECM	1192:1194	arg1	presence					1180:1187	the presence	1176:1187	the presence of ECM compared to PEDOT:PSS alone	1176:1222	SH-SY5Y human neuroblastoma cells show enhanced cell survival and proliferation in the presence of ECM compared to PEDOT:PSS alone.
35904257	7	9	theme	patch-clamp	1236:1246	arg1	recordings					1248:1257	Whole-cell patch-clamp recordings	1225:1257	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds	1225:1316	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds demonstrate that ECM constituents promote neuronal differentiation in situ.
35904257	2	10	theme	natural	305:311	arg1	biomaterials					313:324	natural biomaterials	305:324	natural biomaterials	305:324	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	1	11	theme	3D	74:75	arg1	formats					90:96	3D cell culture formats	74:96	3D cell culture formats	74:96	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	3	12	contain	containing	659:668	arg1	PEDOT					647:651	PEDOT	647:651	PEDOT:PSS	647:655	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	3	12	contain	containing	659:668	arg1	sulfonate					636:644	polystyrene sulfonate	624:644	polystyrene sulfonate (PEDOT:PSS)	624:656	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	3	12	contain	containing	659:668	arg2	components					674:683	ECM components	670:683	ECM components (i.e., collagen, hyaluronic acid, and laminin)	670:730	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	7	13	theme	ECM	1335:1337	arg1	constituents					1339:1350	ECM constituents	1335:1350	ECM constituents	1335:1350	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds demonstrate that ECM constituents promote neuronal differentiation in situ.
35904257	7	14	theme	Whole-cell	1225:1234	arg1	recordings					1248:1257	Whole-cell patch-clamp recordings	1225:1257	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds	1225:1316	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds demonstrate that ECM constituents promote neuronal differentiation in situ.
35904257	2	15	theme	physical	443:450	arg1	cues					452:455	the chemical and physical cues	426:455	the chemical and physical cues of the natural extracellular matrix (ECM)	426:497	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	1	16	theme	cell	77:80	arg1	formats					90:96	3D cell culture formats	74:96	3D cell culture formats	74:96	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	6	17	theme	SH-SY5Y	1093:1099	arg1	cells					1121:1125	SH-SY5Y human neuroblastoma cells	1093:1125	SH-SY5Y human neuroblastoma cells	1093:1125	SH-SY5Y human neuroblastoma cells show enhanced cell survival and proliferation in the presence of ECM compared to PEDOT:PSS alone.
35904257	0	18	theme	Cell	52:55	arg1	Differentiation					57:71	Human Neuronal Cell Differentiation	37:71	Human Neuronal Cell Differentiation	37:71	Conducting Polymer-ECM Scaffolds for Human Neuronal Cell Differentiation.
35904257	4	19	theme	good	842:845	arg1	conductivity					847:858	good conductivity	842:858	good conductivity	842:858	The resulting scaffolds exhibit a highly porous microstructure and good conductivity, determined by scanning electron microscopy and electrochemical impedance spectroscopy, respectively.
35904257	1	20	theme	culture	82:88	arg1	formats					90:96	3D cell culture formats	74:96	3D cell culture formats	74:96	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	6	21	theme	cell	1141:1144	arg1	survival					1146:1153	enhanced cell survival	1132:1153	enhanced cell survival	1132:1153	SH-SY5Y human neuroblastoma cells show enhanced cell survival and proliferation in the presence of ECM compared to PEDOT:PSS alone.
35904257	0	22	theme	Polymer-ECM	11:21	arg1	Scaffolds					23:31	Polymer-ECM Scaffolds	11:31	Polymer-ECM Scaffolds	11:31	Conducting Polymer-ECM Scaffolds for Human Neuronal Cell Differentiation.
35904257	3	23	theme	3D	542:543	arg1	scaffolds					555:563	porous 3D composite scaffolds	535:563	porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin)	535:730	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	6	24	theme	enhanced	1132:1139	arg1	survival					1146:1153	enhanced cell survival	1132:1153	enhanced cell survival	1132:1153	SH-SY5Y human neuroblastoma cells show enhanced cell survival and proliferation in the presence of ECM compared to PEDOT:PSS alone.
35904257	5	25	theme	uptake	1024:1029	arg1	capacity					1031:1038	water uptake capacity	1018:1038	water uptake capacity	1018:1038	These supports boast excellent mechanical stability and water uptake capacity, making them ideal candidates for cell infiltration.
35904257	6	26	dep	PEDOT	1208:1212	arg1	PSS					1214:1216	PSS	1214:1216	PEDOT:PSS alone	1208:1222	SH-SY5Y human neuroblastoma cells show enhanced cell survival and proliferation in the presence of ECM compared to PEDOT:PSS alone.
35904257	2	27	theme	natural	464:470	arg1	ECM					494:496	ECM	494:496	ECM	494:496	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	2	27	theme	natural	464:470	arg1	matrix					486:491	the natural extracellular matrix	460:491	the natural extracellular matrix (ECM)	460:497	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	3	28	dep	collagen	692:699	arg1	i.e.					686:689	i.e.	686:689	i.e.	686:689	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	5	29	theme	boast	977:981	arg1	stability					1004:1012	boast excellent mechanical stability	977:1012	boast excellent mechanical stability	977:1012	These supports boast excellent mechanical stability and water uptake capacity, making them ideal candidates for cell infiltration.
35904257	3	30	theme	ECM	670:672	arg1	components					674:683	ECM components	670:683	ECM components (i.e., collagen, hyaluronic acid, and laminin)	670:730	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	3	31	theme	hosting	751:757	arg1	cells					768:772	hosting neuronal cells	751:772	hosting neuronal cells	751:772	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	5	32	theme	excellent	983:991	arg1	stability					1004:1012	boast excellent mechanical stability	977:1012	boast excellent mechanical stability	977:1012	These supports boast excellent mechanical stability and water uptake capacity, making them ideal candidates for cell infiltration.
35904257	4	33	theme	porous	816:821	arg1	microstructure					823:836	a highly porous microstructure	807:836	a highly porous microstructure	807:836	The resulting scaffolds exhibit a highly porous microstructure and good conductivity, determined by scanning electron microscopy and electrochemical impedance spectroscopy, respectively.
35904257	3	34	dep	PEDOT	647:651	arg1	PSS					653:655	PSS	653:655	PEDOT:PSS	647:655	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	8	35	theme	conducting	1439:1448	arg1	usability					1423:1431	the usability	1419:1431	the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling	1419:1564	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	3	36	theme	composite	545:553	arg1	scaffolds					555:563	porous 3D composite scaffolds	535:563	porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin)	535:730	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	8	37	dep	conducting	1439:1448	arg1	supports					1450:1457	supports	1450:1457	supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling	1450:1564	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	5	38	theme	mechanical	993:1002	arg1	stability					1004:1012	boast excellent mechanical stability	977:1012	boast excellent mechanical stability	977:1012	These supports boast excellent mechanical stability and water uptake capacity, making them ideal candidates for cell infiltration.
35904257	1	39	theme	cell-cell	198:206	arg1	interactions					234:245	the cell-cell and cell-microenvironment interactions	194:245	the cell-cell and cell-microenvironment interactions of the in vivo milieu	194:267	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	0	40	theme	Neuronal	43:50	arg1	Differentiation					57:71	Human Neuronal Cell Differentiation	37:71	Human Neuronal Cell Differentiation	37:71	Conducting Polymer-ECM Scaffolds for Human Neuronal Cell Differentiation.
35904257	3	41	theme	freeze-drying	510:522	arg1	technique					524:532	the freeze-drying technique	506:532	the freeze-drying technique	506:532	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	1	42	dep	in	254:255	arg1	vivo					257:260	vivo	257:260	vivo	257:260	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	3	43	theme	polystyrene	624:634	arg1	PEDOT					647:651	PEDOT	647:651	PEDOT:PSS	647:655	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	3	43	theme	polystyrene	624:634	arg1	sulfonate					636:644	polystyrene sulfonate	624:644	polystyrene sulfonate (PEDOT:PSS)	624:656	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	6	44	theme	neuroblastoma	1107:1119	arg1	cells					1121:1125	SH-SY5Y human neuroblastoma cells	1093:1125	SH-SY5Y human neuroblastoma cells	1093:1125	SH-SY5Y human neuroblastoma cells show enhanced cell survival and proliferation in the presence of ECM compared to PEDOT:PSS alone.
35904257	7	45	theme	neuronal	1360:1367	arg1	differentiation					1369:1383	neuronal differentiation	1360:1383	neuronal differentiation	1360:1383	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds demonstrate that ECM constituents promote neuronal differentiation in situ.
35904257	0	46	theme	Human	37:41	arg1	Differentiation					57:71	Human Neuronal Cell Differentiation	37:71	Human Neuronal Cell Differentiation	37:71	Conducting Polymer-ECM Scaffolds for Human Neuronal Cell Differentiation.
35904257	8	47	theme	disease	1549:1555	arg1	modeling					1557:1564	disease modeling	1549:1564	disease modeling	1549:1564	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	1	48	theme	cell-microenvironment	212:232	arg1	interactions					234:245	the cell-cell and cell-microenvironment interactions	194:245	the cell-cell and cell-microenvironment interactions of the in vivo milieu	194:267	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	8	49	dep	in	1506:1507	arg1	vitro					1509:1513	vitro	1509:1513	vitro	1509:1513	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	4	50	theme	electrochemical	908:922	arg1	spectroscopy					934:945	electrochemical impedance spectroscopy	908:945	electrochemical impedance spectroscopy	908:945	The resulting scaffolds exhibit a highly porous microstructure and good conductivity, determined by scanning electron microscopy and electrochemical impedance spectroscopy, respectively.
35904257	5	51	theme	water	1018:1022	arg1	capacity					1031:1038	water uptake capacity	1018:1038	water uptake capacity	1018:1038	These supports boast excellent mechanical stability and water uptake capacity, making them ideal candidates for cell infiltration.
35904257	8	52	theme	functional	1495:1504	arg1	platforms					1527:1535	engineered highly biomimetic and functional in vitro tissue-like platforms	1462:1535	engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling	1462:1564	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	1	53	theme	tissue	120:125	arg1	complexity					140:149	tissue architecture complexity	120:149	tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu	120:267	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	2	54	theme	matrix	486:491	arg1	cues					452:455	the chemical and physical cues	426:455	the chemical and physical cues of the natural extracellular matrix (ECM)	426:497	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	3	55	theme	neuronal	759:766	arg1	cells					768:772	hosting neuronal cells	751:772	hosting neuronal cells	751:772	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	5	56	theme	cell	1074:1077	arg1	infiltration					1079:1090	cell infiltration	1074:1090	cell infiltration	1074:1090	These supports boast excellent mechanical stability and water uptake capacity, making them ideal candidates for cell infiltration.
35904257	1	57	theme	architecture	127:138	arg1	complexity					140:149	tissue architecture complexity	120:149	tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu	120:267	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	2	58	theme	Scaffold-based	270:283	arg1	systems					285:291	Scaffold-based systems	270:291	Scaffold-based systems integrating natural biomaterials	270:324	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	8	59	theme	engineered	1462:1471	arg1	platforms					1527:1535	engineered highly biomimetic and functional in vitro tissue-like platforms	1462:1535	engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling	1462:1564	These findings reinforce the usability of 3D conducting supports as engineered highly biomimetic and functional in vitro tissue-like platforms for drug or disease modeling.
35904257	4	60	theme	impedance	924:932	arg1	spectroscopy					934:945	electrochemical impedance spectroscopy	908:945	electrochemical impedance spectroscopy	908:945	The resulting scaffolds exhibit a highly porous microstructure and good conductivity, determined by scanning electron microscopy and electrochemical impedance spectroscopy, respectively.
35904257	4	61	theme	resulting	779:787	arg1	scaffolds					789:797	The resulting scaffolds	775:797	The resulting scaffolds	775:797	The resulting scaffolds exhibit a highly porous microstructure and good conductivity, determined by scanning electron microscopy and electrochemical impedance spectroscopy, respectively.
35904257	2	62	theme	tissue	354:359	arg1	engineering					361:371	tissue engineering	354:371	tissue engineering	354:371	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	4	63	theme	electron	884:891	arg1	microscopy					893:902	scanning electron microscopy	875:902	scanning electron microscopy	875:902	The resulting scaffolds exhibit a highly porous microstructure and good conductivity, determined by scanning electron microscopy and electrochemical impedance spectroscopy, respectively.
35904257	7	64	theme	SHSY5Y	1288:1293	arg1	cells					1295:1299	differentiated SHSY5Y cells	1273:1299	differentiated SHSY5Y cells	1273:1299	Whole-cell patch-clamp recordings acquired from differentiated SHSY5Y cells in the scaffolds demonstrate that ECM constituents promote neuronal differentiation in situ.
35904257	2	65	theme	chemical	430:437	arg1	cues					452:455	the chemical and physical cues	426:455	the chemical and physical cues of the natural extracellular matrix (ECM)	426:497	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	6	66	theme	human	1101:1105	arg1	cells					1121:1125	SH-SY5Y human neuroblastoma cells	1093:1125	SH-SY5Y human neuroblastoma cells	1093:1125	SH-SY5Y human neuroblastoma cells show enhanced cell survival and proliferation in the presence of ECM compared to PEDOT:PSS alone.
35904257	1	67	theme	2D	156:157	arg1	systems					159:165	2D systems	156:165	2D systems	156:165	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35904257	3	68	theme	hyaluronic	702:711	arg1	acid					713:716	hyaluronic acid	702:716	hyaluronic acid	702:716	Using the freeze-drying technique, porous 3D composite scaffolds consisting of poly(3,4-ethylene-dioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS), containing ECM components (i.e., collagen, hyaluronic acid, and laminin) are engineered for hosting neuronal cells.
35904257	2	69	theme	extracellular	472:484	arg1	ECM					494:496	ECM	494:496	ECM	494:496	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	2	69	theme	extracellular	472:484	arg1	matrix					486:491	the natural extracellular matrix	460:491	the natural extracellular matrix (ECM)	460:497	Scaffold-based systems integrating natural biomaterials are extensively employed in tissue engineering to improve cell survival and outgrowth, by providing the chemical and physical cues of the natural extracellular matrix (ECM).
35904257	1	70	theme	in	254:255	arg1	milieu					262:267	the in vivo milieu	250:267	the in vivo milieu	250:267	3D cell culture formats more closely resemble tissue architecture complexity than 2D systems, which are lacking most of the cell-cell and cell-microenvironment interactions of the in vivo milieu.
35689007	0	0	theme	hemodialysis	76:87	arg1	patients					89:96	hemodialysis patients	76:96	hemodialysis patients	76:96	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.
35689007	4	1	theme	dental	500:505	arg1	plaque					507:512	dental plaque	500:512	dental plaque	500:512	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	3	2	dep	binder	380:385	arg1	SFOH					413:416	SFOH	413:416	SFOH	413:416	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	3	2	dep	binder	380:385	arg1	oxyhydroxide					399:410	sucroferric oxyhydroxide	387:410	the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH)	353:417	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	10	3	theme	iron	1282:1285	arg1	plethora					1287:1294	iron plethora	1282:1294	iron plethora	1282:1294	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	10	4	contain	had	1296:1298	arg2	siderophores					1316:1327	no iron-binding siderophores	1300:1327	no iron-binding siderophores	1300:1327	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	10	4	contain	had	1296:1298	arg1	taxa					1243:1246	taxa	1243:1246	taxa that significantly benefited from iron plethora	1243:1294	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	10	4	contain	had	1296:1298	arg2	alternatives					1332:1343	alternatives	1332:1343	alternatives	1332:1343	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	10	5	dep	taxa	1371:1374	arg1	contrast					1359:1366	contrast	1359:1366	contrast	1359:1366	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	0	6	from	Impact	0:5	arg1	microbiome					62:71	the oral and intestinal microbiome	38:71	the oral and intestinal microbiome in hemodialysis patients	38:96	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.
35689007	12	7	from	impact	1575:1580	arg1	microbiome					1597:1606	the microbiome	1593:1606	the microbiome of hemodialysis patients	1593:1631	In conclusion, this study sheds light on the impact of iron on the microbiome of hemodialysis patients.
35689007	4	8	theme	intake	579:584	arg1	weeks					565:569	one and four weeks	552:569	one and four weeks of SFOH intake	552:584	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	4	8	theme	intake	579:584	arg1	baseline					542:549	baseline	542:549	baseline	542:549	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	3	9	theme	-based	363:368	arg1	binder					380:385	the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH)	353:417	the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH)	353:417	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	9	10	theme	unidirectional	1159:1172	arg1	changes					1174:1180	significant unidirectional changes	1147:1180	significant unidirectional changes	1147:1180	Eight bacterial taxa showed significant unidirectional changes during treatment.
35689007	10	11	theme	microbiome	1209:1218	arg1	analysis					1220:1227	In-depth microbiome analysis	1200:1227	In-depth microbiome analysis	1200:1227	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	12	12	theme	patients	1624:1631	arg1	microbiome					1597:1606	the microbiome	1593:1606	the microbiome of hemodialysis patients	1593:1631	In conclusion, this study sheds light on the impact of iron on the microbiome of hemodialysis patients.
35689007	8	13	dep	Total	988:992	arg1	Fe					994:995	Fe	994:995	Fe	994:995	Total Fe and Fe(2+) were highest after one week of SFOH, particularly in patients who exhibited a shift in microbiome composition.
35689007	4	14	theme	SFOH	574:577	arg1	intake					579:584	SFOH intake	574:584	SFOH intake	574:584	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	4	15	theme	rRNA	607:610	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	2	16	theme	phosphate	301:309	arg1	binders					311:317	phosphate binders	301:317	phosphate binders	301:317	Therapeutically, most dialysis patients have to take phosphate binders.
35689007	8	17	theme	SFOH	1039:1042	arg1	week					1031:1034	one week	1027:1034	one week of SFOH	1027:1042	Total Fe and Fe(2+) were highest after one week of SFOH, particularly in patients who exhibited a shift in microbiome composition.
35689007	1	18	theme	mineral/bone	176:187	arg1	impairment					189:198	mineral/bone impairment	176:198	mineral/bone impairment	176:198	Hyperphosphatemia is a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality.
35689007	9	19	theme	significant	1147:1157	arg1	changes					1174:1180	significant unidirectional changes	1147:1180	significant unidirectional changes	1147:1180	Eight bacterial taxa showed significant unidirectional changes during treatment.
35689007	10	20	theme	iron-binding	1303:1314	arg1	siderophores					1316:1327	no iron-binding siderophores	1300:1327	no iron-binding siderophores	1300:1327	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	3	21	theme	gastrointestinal	435:450	arg1	microbiome					452:461	the oral and gastrointestinal microbiome	422:461	the oral and gastrointestinal microbiome of 11 hemodialysis patients	422:489	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	3	22	theme	sucroferric	387:397	arg1	SFOH					413:416	SFOH	413:416	SFOH	413:416	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	3	22	theme	sucroferric	387:397	arg1	oxyhydroxide					399:410	sucroferric oxyhydroxide	387:410	the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH)	353:417	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	11	23	theme	higher	1491:1496	arg1	concentrations					1514:1527	higher serum phosphate concentrations	1491:1527	higher serum phosphate concentrations	1491:1527	Patients with microbiome-shift were significantly younger and had higher serum phosphate concentrations.
35689007	4	24	theme	Illumina	641:648	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	12	25	theme	hemodialysis	1611:1622	arg1	patients					1624:1631	hemodialysis patients	1611:1631	hemodialysis patients	1611:1631	In conclusion, this study sheds light on the impact of iron on the microbiome of hemodialysis patients.
35689007	8	26	theme	microbiome	1095:1104	arg1	composition					1106:1116	microbiome composition	1095:1116	microbiome composition	1095:1116	Total Fe and Fe(2+) were highest after one week of SFOH, particularly in patients who exhibited a shift in microbiome composition.
35689007	1	27	theme	increased	201:209	arg1	events					226:231	increased cardiovascular events	201:231	increased cardiovascular events	201:231	Hyperphosphatemia is a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality.
35689007	3	28	theme	phosphate	370:378	arg1	binder					380:385	the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH)	353:417	the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH)	353:417	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	12	29	theme	iron	1585:1588	arg1	impact					1575:1580	the impact	1571:1580	the impact of iron on the microbiome of hemodialysis patients	1571:1631	In conclusion, this study sheds light on the impact of iron on the microbiome of hemodialysis patients.
35689007	1	30	theme	cardiovascular	211:224	arg1	events					226:231	increased cardiovascular events	201:231	increased cardiovascular events	201:231	Hyperphosphatemia is a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality.
35689007	3	31	theme	binder	380:385	arg1	effects					342:348	effects	342:348	effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients	342:489	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	3	32	theme	hemodialysis	469:480	arg1	patients					482:489	11 hemodialysis patients	466:489	11 hemodialysis patients	466:489	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	7	33	from	shift	921:925	arg1	6/11					948:951	6/11	948:951	6/11	948:951	However, some patient-, sample- and taxon-specific differences were noted, which allowed patients to be divided into those with a shift in their microbiome (6/11) and those without a shift (5/11).
35689007	7	33	from	shift	921:925	arg1	microbiome					936:945	their microbiome	930:945	their microbiome (6/11)	930:952	However, some patient-, sample- and taxon-specific differences were noted, which allowed patients to be divided into those with a shift in their microbiome (6/11) and those without a shift (5/11).
35689007	2	34	theme	most	265:268	arg1	patients					279:286	most dialysis patients	265:286	most dialysis patients	265:286	Therapeutically, most dialysis patients have to take phosphate binders.
35689007	3	35	theme	oral	426:429	arg1	microbiome					452:461	the oral and gastrointestinal microbiome	422:461	the oral and gastrointestinal microbiome of 11 hemodialysis patients	422:489	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	4	36	theme	V3-V4	618:622	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	0	37	theme	sucroferric	10:20	arg1	oxyhydroxide					22:33	sucroferric oxyhydroxide	10:33	sucroferric oxyhydroxide	10:33	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.
35689007	0	38	from	microbiome	62:71	arg1	patients					89:96	hemodialysis patients	76:96	hemodialysis patients	76:96	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.
35689007	0	39	theme	oxyhydroxide	22:33	arg1	Impact					0:5	Impact	0:5	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.	0:97	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.
35689007	11	40	theme	serum	1498:1502	arg1	concentrations					1514:1527	higher serum phosphate concentrations	1491:1527	higher serum phosphate concentrations	1491:1527	Patients with microbiome-shift were significantly younger and had higher serum phosphate concentrations.
35689007	11	41	theme	phosphate	1504:1512	arg1	concentrations					1514:1527	higher serum phosphate concentrations	1491:1527	higher serum phosphate concentrations	1491:1527	Patients with microbiome-shift were significantly younger and had higher serum phosphate concentrations.
35689007	11	42	contain	had	1487:1489	arg1	Patients					1425:1432	Patients	1425:1432	Patients with microbiome-shift	1425:1454	Patients with microbiome-shift were significantly younger and had higher serum phosphate concentrations.
35689007	11	42	contain	had	1487:1489	arg2	concentrations					1514:1527	higher serum phosphate concentrations	1491:1527	higher serum phosphate concentrations	1491:1527	Patients with microbiome-shift were significantly younger and had higher serum phosphate concentrations.
35689007	8	43	from	shift	1086:1090	arg1	composition					1106:1116	microbiome composition	1095:1116	microbiome composition	1095:1116	Total Fe and Fe(2+) were highest after one week of SFOH, particularly in patients who exhibited a shift in microbiome composition.
35689007	0	44	theme	oral	42:45	arg1	microbiome					62:71	the oral and intestinal microbiome	38:71	the oral and intestinal microbiome in hemodialysis patients	38:96	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.
35689007	10	45	theme	iron	1410:1413	arg1	plethora					1415:1422	iron plethora	1410:1422	iron plethora	1410:1422	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	3	46	from	effects	342:348	arg1	microbiome					452:461	the oral and gastrointestinal microbiome	422:461	the oral and gastrointestinal microbiome of 11 hemodialysis patients	422:489	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	10	47	theme	In-depth	1200:1207	arg1	analysis					1220:1227	In-depth microbiome analysis	1200:1227	In-depth microbiome analysis	1200:1227	In-depth microbiome analysis revealed that taxa that significantly benefited from iron plethora had no iron-binding siderophores or alternatives, which was in contrast to taxa that significantly declined under iron plethora.
35689007	4	48	theme	directed	632:639	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	7	49	theme	taxon-specific	827:840	arg1	differences					842:852	some patient-, sample- and taxon-specific differences	800:852	some patient-, sample- and taxon-specific differences	800:852	However, some patient-, sample- and taxon-specific differences were noted, which allowed patients to be divided into those with a shift in their microbiome (6/11) and those without a shift (5/11).
35689007	4	50	theme	MiSeq-based	650:660	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	3	51	theme	patients	482:489	arg1	microbiome					452:461	the oral and gastrointestinal microbiome	422:461	the oral and gastrointestinal microbiome of 11 hemodialysis patients	422:489	Here, we investigated effects of the Fe(3+)-based phosphate binder sucroferric oxyhydroxide (SFOH) on the oral and gastrointestinal microbiome of 11 hemodialysis patients.
35689007	5	52	theme	Total	672:676	arg1	Fe					678:679	Total Fe	672:679	Total Fe	672:679	Total Fe, Fe(2+) and Fe(3+) were determined in stool and saliva.
35689007	1	53	theme	chronic	137:143	arg1	disease					152:158	chronic kidney disease	137:158	chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality	137:245	Hyperphosphatemia is a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality.
35689007	7	54	theme	sample-	815:821	arg1	differences					842:852	some patient-, sample- and taxon-specific differences	800:852	some patient-, sample- and taxon-specific differences	800:852	However, some patient-, sample- and taxon-specific differences were noted, which allowed patients to be divided into those with a shift in their microbiome (6/11) and those without a shift (5/11).
35689007	4	55	theme	gene	612:615	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	1	56	theme	kidney	145:150	arg1	disease					152:158	chronic kidney disease	137:158	chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality	137:245	Hyperphosphatemia is a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality.
35689007	4	57	theme	region	624:629	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	4	58	theme	16S	603:605	arg1	analysis					662:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis	603:669	Saliva, dental plaque and stool were collected at baseline, one and four weeks of SFOH intake and subjected to 16S rRNA gene (V3-V4 region) directed Illumina MiSeq-based analysis.
35689007	0	59	theme	intestinal	51:60	arg1	microbiome					62:71	the oral and intestinal microbiome	38:71	the oral and intestinal microbiome in hemodialysis patients	38:96	Impact of sucroferric oxyhydroxide on the oral and intestinal microbiome in hemodialysis patients.
35689007	9	60	theme	bacterial	1125:1133	arg1	taxa					1135:1138	Eight bacterial taxa	1119:1138	Eight bacterial taxa	1119:1138	Eight bacterial taxa showed significant unidirectional changes during treatment.
35689007	2	61	theme	dialysis	270:277	arg1	patients					279:286	most dialysis patients	265:286	most dialysis patients	265:286	Therapeutically, most dialysis patients have to take phosphate binders.
35689007	1	62	theme	disease	152:158	arg1	Hyperphosphatemia					99:115	Hyperphosphatemia	99:115	Hyperphosphatemia	99:115	Hyperphosphatemia is a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality.
35689007	1	62	theme	disease	152:158	arg1	consequence					122:132	a consequence	120:132	a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality	120:245	Hyperphosphatemia is a consequence of chronic kidney disease associated with mineral/bone impairment, increased cardiovascular events and mortality.
35689007	7	63	theme	patient-	805:812	arg1	differences					842:852	some patient-, sample- and taxon-specific differences	800:852	some patient-, sample- and taxon-specific differences	800:852	However, some patient-, sample- and taxon-specific differences were noted, which allowed patients to be divided into those with a shift in their microbiome (6/11) and those without a shift (5/11).
35689007	11	64	with	Patients	1425:1432	arg1	microbiome-shift					1439:1454	microbiome-shift	1439:1454	microbiome-shift	1439:1454	Patients with microbiome-shift were significantly younger and had higher serum phosphate concentrations.
37263085	7	0	from	diseases	1571:1578	arg1	homeostasis					1504:1514	intestinal microbiota homeostasis	1482:1514	intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases	1482:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	5	1	theme	proteins	1195:1202	arg1	abundance					1134:1142	the abundance	1130:1142	the abundance of ABC transporters, DNA repair, and recombination proteins	1130:1202	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	7	2	theme	intestinal	1482:1491	arg1	homeostasis					1504:1514	intestinal microbiota homeostasis	1482:1514	intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases	1482:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	0	3	from	inflammation	144:155	arg1	mice					172:175	peritonitis mice	160:175	peritonitis mice	160:175	Pickering emulsion stabilized by parasin I and chitosan nanoparticles enhances protection against intestinal microbiota homeostasis by reducing inflammation in peritonitis mice.
37263085	3	4	theme	NOD-	874:877	arg1	expression					860:869	the expression	856:869	the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3)	856:929	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	2	5	theme	intestinal	500:509	arg1	microbiota					511:520	the intestinal microbiota	496:520	the intestinal microbiota	496:520	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	2	6	from	function	472:479	arg1	inflammation					526:537	inflammation	526:537	inflammation	526:537	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	2	6	from	function	472:479	arg1	microbiota					511:520	the intestinal microbiota	496:520	the intestinal microbiota	496:520	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	5	7	theme	emulsion	1094:1101	arg1	groups					1110:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	7	8	from	emulsion	1538:1545	arg1	diseases					1571:1578	inflammation-related diseases	1550:1578	inflammation-related diseases	1550:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	1	9	theme	various	187:193	arg1	researches					195:204	various researches	187:204	various researches	187:204	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	3	10	theme	protein	913:919	arg1	expression					860:869	the expression	856:869	the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3)	856:929	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	1	11	theme	few	290:292	arg1	studies					294:300	few studies	290:300	few studies	290:300	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	0	12	theme	microbiota	109:118	arg1	homeostasis					120:130	intestinal microbiota homeostasis	98:130	intestinal microbiota homeostasis	98:130	Pickering emulsion stabilized by parasin I and chitosan nanoparticles enhances protection against intestinal microbiota homeostasis by reducing inflammation in peritonitis mice.
37263085	1	13	dep	role	315:318	arg1	the					311:313	the	311:313	the	311:313	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	6	14	theme	protection	1284:1293	arg1	effect					1295:1300	a better protection effect	1275:1300	a better protection effect	1275:1300	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	5	15	theme	recombination	1181:1193	arg1	proteins					1195:1202	recombination proteins	1181:1202	recombination proteins	1181:1202	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	0	16	theme	intestinal	98:107	arg1	homeostasis					120:130	intestinal microbiota homeostasis	98:130	intestinal microbiota homeostasis	98:130	Pickering emulsion stabilized by parasin I and chitosan nanoparticles enhances protection against intestinal microbiota homeostasis by reducing inflammation in peritonitis mice.
37263085	7	17	theme	microbiota	1493:1502	arg1	homeostasis					1504:1514	intestinal microbiota homeostasis	1482:1514	intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases	1482:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	6	18	theme	better	1277:1282	arg1	effect					1295:1300	a better protection effect	1275:1300	a better protection effect	1275:1300	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	7	19	theme	Pickering	1528:1536	arg1	emulsion					1538:1545	chitosan Pickering emulsion	1519:1545	chitosan Pickering emulsion in inflammation-related diseases	1519:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	6	20	theme	intestinal	1337:1346	arg1	microbiota					1348:1357	intestinal microbiota	1337:1357	intestinal microbiota	1337:1357	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	5	21	theme	microbial	1221:1229	arg1	gut					1217:1219	gut microbial	1217:1229	gut microbial	1217:1229	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	4	22	theme	fibrosis	995:1002	arg1	area					1004:1007	a smaller fibrosis area	985:1007	a smaller fibrosis area in the spleen	985:1021	The CPENE showed better histological morphology with a smaller fibrosis area in the spleen.
37263085	2	23	theme	antimicrobial	433:445	arg1	emulsions					457:465	our previously published natural and antimicrobial Pickering emulsions	396:465	our previously published natural and antimicrobial Pickering emulsions	396:465	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	2	24	theme	published	411:419	arg1	emulsions					457:465	our previously published natural and antimicrobial Pickering emulsions	396:465	our previously published natural and antimicrobial Pickering emulsions	396:465	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	4	25	theme	smaller	987:993	arg1	area					1004:1007	a smaller fibrosis area	985:1007	a smaller fibrosis area in the spleen	985:1021	The CPENE showed better histological morphology with a smaller fibrosis area in the spleen.
37263085	1	26	from	mechanism	324:332	arg1	homeostasis					360:370	gut flora homeostasis	350:370	gut flora homeostasis	350:370	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	7	27	theme	inflammation-related	1550:1569	arg1	diseases					1571:1578	inflammation-related diseases	1550:1578	inflammation-related diseases	1550:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	6	28	theme	interleukin-1β	1373:1386	arg1	secretion					1388:1396	interleukin-1β secretion	1373:1396	interleukin-1β secretion	1373:1396	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	6	29	dep	composition	1309:1319	arg1	the					1305:1307	the	1305:1307	the	1305:1307	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	3	30	theme	parasin	763:769	arg1	I					771:771	parasin I	763:771	parasin I in peritoneal tissue	763:792	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	6	31	theme	inflammasome	1418:1429	arg1	secretion					1388:1396	interleukin-1β secretion	1373:1396	interleukin-1β secretion	1373:1396	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	6	31	theme	inflammasome	1418:1429	arg1	assembly					1402:1409	assembly	1402:1409	assembly of the inflammasome of NLRP3	1402:1438	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	6	32	theme	Pickering	1249:1257	arg1	emulsion					1259:1266	the Pickering emulsion	1245:1266	the Pickering emulsion	1245:1266	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	3	33	dep	nanoparticles	633:645	arg1	LPNE					657:660	LPNE	657:660	LPNE	657:660	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	33	dep	nanoparticles	633:645	arg1	emulsion					647:654	emulsion	647:654	emulsion (LPNE)	647:661	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	0	34	theme	Pickering	0:8	arg1	emulsion					10:17	Pickering emulsion	0:17	Pickering emulsion stabilized by parasin I and chitosan nanoparticles	0:68	Pickering emulsion stabilized by parasin I and chitosan nanoparticles enhances protection against intestinal microbiota homeostasis by reducing inflammation in peritonitis mice.
37263085	1	35	from	role	315:318	arg1	homeostasis					360:370	gut flora homeostasis	350:370	gut flora homeostasis	350:370	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	7	36	theme	emulsion	1538:1545	arg1	homeostasis					1504:1514	intestinal microbiota homeostasis	1482:1514	intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases	1482:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	3	37	theme	peritoneal	818:827	arg1	inflammation					829:840	peritoneal inflammation	818:840	peritoneal inflammation	818:840	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	38	from	I	771:771	arg1	tissue					787:792	peritoneal tissue	776:792	peritoneal tissue	776:792	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	39	dep	less	738:741	arg1	fiber					752:756	collagen fiber	743:756	collagen fiber	743:756	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	4	40	theme	better	949:954	arg1	morphology					969:978	better histological morphology	949:978	better histological morphology	949:978	The CPENE showed better histological morphology with a smaller fibrosis area in the spleen.
37263085	3	41	theme	lipid/peptide	619:631	arg1	nanoparticles					633:645	lipid/peptide nanoparticles	619:645	lipid/peptide nanoparticles	619:645	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	2	42	theme	Pickering	447:455	arg1	emulsions					457:465	our previously published natural and antimicrobial Pickering emulsions	396:465	our previously published natural and antimicrobial Pickering emulsions	396:465	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	2	43	theme	emulsions	457:465	arg1	basics					386:391	the basics	382:391	the basics of our previously published natural and antimicrobial Pickering emulsions	382:465	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	3	44	theme	peritoneal	776:785	arg1	tissue					787:792	peritoneal tissue	776:792	peritoneal tissue	776:792	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	6	45	theme	microbiota	1348:1357	arg1	function					1325:1332	function	1325:1332	function	1325:1332	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	6	45	theme	microbiota	1348:1357	arg1	composition					1309:1319	composition	1309:1319	composition	1309:1319	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	3	46	theme	peptide-embedded	680:695	arg1	CPENE					721:725	CPENE	721:725	CPENE	721:725	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	46	theme	peptide-embedded	680:695	arg1	emulsion					711:718	the chitosan peptide-embedded nanoparticles emulsion	667:718	the chitosan peptide-embedded nanoparticles emulsion (CPENE)	667:726	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	7	47	from	homeostasis	1504:1514	arg1	diseases					1571:1578	inflammation-related diseases	1550:1578	inflammation-related diseases	1550:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	1	48	theme	drug	234:237	arg1	efficiency					247:256	drug loading efficiency	234:256	drug loading efficiency	234:256	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	0	49	theme	peritonitis	160:170	arg1	mice					172:175	peritonitis mice	160:175	peritonitis mice	160:175	Pickering emulsion stabilized by parasin I and chitosan nanoparticles enhances protection against intestinal microbiota homeostasis by reducing inflammation in peritonitis mice.
37263085	3	50	theme	nanoparticles	697:709	arg1	CPENE					721:725	CPENE	721:725	CPENE	721:725	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	50	theme	nanoparticles	697:709	arg1	emulsion					711:718	the chitosan peptide-embedded nanoparticles emulsion	667:718	the chitosan peptide-embedded nanoparticles emulsion (CPENE)	667:726	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	1	51	theme	loading	239:245	arg1	efficiency					247:256	drug loading efficiency	234:256	drug loading efficiency	234:256	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	3	52	theme	pyrin	889:893	arg1	protein					913:919	pyrin domain-containing protein 3	889:921	pyrin domain-containing protein 3 (NLRP3)	889:929	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	52	theme	pyrin	889:893	arg1	NLRP3					924:928	NLRP3	924:928	NLRP3	924:928	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	2	53	theme	Kunming	564:570	arg1	mice					572:575	Kunming mice	564:575	Kunming mice with peritonitis	564:592	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	5	54	theme	PCNE	1104:1107	arg1	groups					1110:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	1	55	dep	stability	220:228	arg1	the					216:218	the	216:218	the	216:218	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	2	56	theme	natural	421:427	arg1	emulsions					457:465	our previously published natural and antimicrobial Pickering emulsions	396:465	our previously published natural and antimicrobial Pickering emulsions	396:465	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	2	57	theme	emulsion	484:491	arg1	function					472:479	the function	468:479	the function of emulsion on the intestinal microbiota and inflammation response	468:546	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	1	58	theme	emulsions	337:345	arg1	mechanism					324:332	mechanism	324:332	mechanism	324:332	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	1	58	theme	emulsions	337:345	arg1	role					315:318	role	315:318	role	315:318	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	2	59	dep	microbiota	511:520	arg1	response					539:546	response	539:546	response	539:546	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	3	60	theme	chitosan	671:678	arg1	CPENE					721:725	CPENE	721:725	CPENE	721:725	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	60	theme	chitosan	671:678	arg1	emulsion					711:718	the chitosan peptide-embedded nanoparticles emulsion	667:718	the chitosan peptide-embedded nanoparticles emulsion (CPENE)	667:726	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	5	61	theme	ABC	1147:1149	arg1	transporters					1151:1162	ABC transporters	1147:1162	ABC transporters	1147:1162	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	4	62	theme	histological	956:967	arg1	morphology					969:978	better histological morphology	949:978	better histological morphology	949:978	The CPENE showed better histological morphology with a smaller fibrosis area in the spleen.
37263085	5	63	theme	repair	1169:1174	arg1	abundance					1134:1142	the abundance	1130:1142	the abundance of ABC transporters, DNA repair, and recombination proteins	1130:1202	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	3	64	theme	collagen	743:750	arg1	fiber					752:756	collagen fiber	743:756	collagen fiber	743:756	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	5	65	theme	parasin	1051:1057	arg1	groups					1110:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	0	66	dep	parasin	33:39	arg1	nanoparticles					56:68	nanoparticles	56:68	nanoparticles	56:68	Pickering emulsion stabilized by parasin I and chitosan nanoparticles enhances protection against intestinal microbiota homeostasis by reducing inflammation in peritonitis mice.
37263085	2	67	with	mice	572:575	arg1	peritonitis					582:592	peritonitis	582:592	peritonitis	582:592	Thus, in the basics of our previously published natural and antimicrobial Pickering emulsions, the function of emulsion on the intestinal microbiota and inflammation response was explored in Kunming mice with peritonitis.
37263085	1	68	theme	gut	350:352	arg1	flora					354:358	gut flora	350:358	gut flora homeostasis	350:370	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	5	69	theme	I-conjugated	1059:1070	arg1	groups					1110:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	7	70	theme	chitosan	1519:1526	arg1	emulsion					1538:1545	chitosan Pickering emulsion	1519:1545	chitosan Pickering emulsion in inflammation-related diseases	1519:1578	These results could provide evidence for intestinal microbiota homeostasis of chitosan Pickering emulsion in inflammation-related diseases.
37263085	3	71	theme	domain-containing	895:911	arg1	protein					913:919	pyrin domain-containing protein 3	889:921	pyrin domain-containing protein 3 (NLRP3)	889:929	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	3	71	theme	domain-containing	895:911	arg1	NLRP3					924:928	NLRP3	924:928	NLRP3	924:928	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	5	72	theme	chitosan	1072:1079	arg1	groups					1110:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	3	73	theme	LRR-	880:883	arg1	expression					860:869	the expression	856:869	the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3)	856:929	The results showed that lipid/peptide nanoparticles emulsion (LPNE) and the chitosan peptide-embedded nanoparticles emulsion (CPENE) presented less collagen fiber than parasin I in peritoneal tissue, and CPENE could reduce peritoneal inflammation by decreasing the expression of NOD-, LRR- and pyrin domain-containing protein 3 (NLRP3).
37263085	1	74	theme	chitosan	261:268	arg1	emulsion					280:287	chitosan Pickering emulsion	261:287	chitosan Pickering emulsion	261:287	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	5	75	theme	DNA	1165:1167	arg1	repair					1169:1174	DNA repair	1165:1174	DNA repair	1165:1174	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	5	76	theme	nanoparticle	1081:1092	arg1	groups					1110:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups	1051:1115	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	5	77	theme	transporters	1151:1162	arg1	abundance					1134:1142	the abundance	1130:1142	the abundance of ABC transporters, DNA repair, and recombination proteins	1130:1202	Moreover, CPENE, LPNE, and parasin I-conjugated chitosan nanoparticle emulsion (PCNE) groups can increase the abundance of ABC transporters, DNA repair, and recombination proteins, and improve gut microbial.
37263085	1	78	theme	Pickering	270:278	arg1	emulsion					280:287	chitosan Pickering emulsion	261:287	chitosan Pickering emulsion	261:287	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	6	79	theme	NLRP3	1434:1438	arg1	inflammasome					1418:1429	the inflammasome	1414:1429	the inflammasome of NLRP3	1414:1438	Furthermore, the Pickering emulsion showed a better protection effect on the composition and function of intestinal microbiota by decreasing interleukin-1β secretion and assembly of the inflammasome of NLRP3.
37263085	1	80	theme	flora	354:358	arg1	homeostasis					360:370	gut flora homeostasis	350:370	gut flora homeostasis	350:370	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	4	81	from	area	1004:1007	arg1	spleen					1016:1021	the spleen	1012:1021	the spleen	1012:1021	The CPENE showed better histological morphology with a smaller fibrosis area in the spleen.
37263085	1	82	theme	emulsion	280:287	arg1	stability					220:228	stability	220:228	stability	220:228	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
37263085	1	82	theme	emulsion	280:287	arg1	efficiency					247:256	drug loading efficiency	234:256	drug loading efficiency	234:256	Although various researches evaluated the stability and drug loading efficiency of chitosan Pickering emulsion, few studies assessed the role and mechanism of emulsions in gut flora homeostasis.
35926672	2	0	theme	further	464:470	arg1	evaluation					478:487	further their evaluation	464:487	further their evaluation for the efficient treatment of effluent	464:527	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	1	1	theme	heavy	331:335	arg1	burden					337:342	a heavy burden	329:342	a heavy burden on the receiving water bodies	329:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	3	2	from	crosslinker	717:727	arg1	synthesis					626:634	the rapid synthesis	616:634	the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles	616:782	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	0	3	theme	dye	98:100	arg1	removal					102:108	methyl orange dye removal	84:108	methyl orange dye removal from unary and complex effluent matrices	84:149	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	3	4	theme	@	658:658	arg1	nanocomposite					639:651	nanocomposite	639:651	nanocomposite (C-CS@ZnO)	639:662	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	3	4	theme	@	658:658	arg1	ZnO					659:661	C-CS@ZnO	654:661	C-CS@ZnO	654:661	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	0	5	link	cross-linked	32:43	arg1	oxide					60:64	cross-linked chitosan-metal oxide	32:64	cross-linked chitosan-metal oxide	32:64	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	6	6	theme	maximum	1153:1159	arg1	185.2 mg g-1					1180:1191	185.2 mg g-1	1180:1191	185.2 mg g-1	1180:1191	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	6	6	theme	maximum	1153:1159	arg1	capacity					1170:1177	The maximum sorption capacity	1149:1177	The maximum sorption capacity (185.2 mg g-1)	1149:1192	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	8	7	theme	large	1586:1590	arg1	scale					1592:1596	a large scale	1584:1596	a large scale for textile effluent treatment	1584:1627	The obtained results suggest its promising applicability at a large scale for textile effluent treatment.
35926672	0	8	theme	unary	115:119	arg1	matrices					142:149	unary and complex effluent matrices	115:149	unary and complex effluent matrices	115:149	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	6	9	theme	sorption	1161:1168	arg1	185.2 mg g-1					1180:1191	185.2 mg g-1	1180:1191	185.2 mg g-1	1180:1191	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	6	9	theme	sorption	1161:1168	arg1	capacity					1170:1177	The maximum sorption capacity	1149:1177	The maximum sorption capacity (185.2 mg g-1)	1149:1192	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	6	10	theme	pseudo-second-order	1342:1360	arg1	R2 ≥ 1					1363:1368	R2 ≥ 1	1363:1368	R2 ≥ 1	1363:1368	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	6	10	theme	pseudo-second-order	1342:1360	arg1	models					1371:1376	Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models	1315:1376	models	1371:1376	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	1	11	theme	large	277:281	arg1	volume					283:288	a large volume	275:288	a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies	275:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	2	12	theme	efficient	497:505	arg1	treatment					507:515	the efficient treatment	493:515	the efficient treatment of effluent	493:527	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	2	13	theme	topics	558:563	arg1	topics					558:563	the most prominent topics	539:563	the most prominent topics	539:563	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	2	13	theme	topics	558:563	arg1	one					532:534	one	532:534	one	532:534	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	2	13	theme	topics	558:563	arg1	identification					390:403	the identification	386:403	the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent	386:527	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	1	14	theme	volume	283:288	arg1	generation					261:270	the generation	257:270	the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies	257:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	0	15	theme	complex	125:131	arg1	matrices					142:149	unary and complex effluent matrices	115:149	unary and complex effluent matrices	115:149	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	1	16	theme	receiving	351:359	arg1	bodies					367:372	the receiving water bodies	347:372	the receiving water bodies	347:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	2	17	theme	methods	408:414	arg1	topics					558:563	the most prominent topics	539:563	the most prominent topics	539:563	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	2	17	theme	methods	408:414	arg1	one					532:534	one	532:534	one	532:534	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	2	17	theme	methods	408:414	arg1	identification					390:403	the identification	386:403	the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent	386:527	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	1	18	theme	water	361:365	arg1	bodies					367:372	the receiving water bodies	347:372	the receiving water bodies	347:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	1	19	from	burden	337:342	arg1	bodies					367:372	the receiving water bodies	347:372	the receiving water bodies	347:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	3	20	from	biomolecule	677:687	arg1	synthesis					626:634	the rapid synthesis	616:634	the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles	616:782	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	8	21	theme	effluent	1610:1617	arg1	treatment					1619:1627	textile effluent treatment	1602:1627	textile effluent treatment	1602:1627	The obtained results suggest its promising applicability at a large scale for textile effluent treatment.
35926672	4	22	dep	composition	866:876	arg1	XPS					886:888	XPS	886:888	XPS	886:888	Detailed characterization was performed to identify the structure (SEM, XRD) and composition (FT-IR, XPS) of the sorbent.
35926672	4	22	dep	composition	866:876	arg1	FT-IR					879:883	FT-IR	879:883	FT-IR	879:883	Detailed characterization was performed to identify the structure (SEM, XRD) and composition (FT-IR, XPS) of the sorbent.
35926672	5	23	theme	dye	1013:1015	arg1	150-350 mg L-1					1033:1046	150-350 mg L-1	1033:1046	150-350 mg L-1	1033:1046	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	5	23	theme	dye	1013:1015	arg1	concentrations					1017:1030	dye concentrations	1013:1030	dye concentrations (150-350 mg L-1)	1013:1047	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	4	24	theme	sorbent	898:904	arg1	composition					866:876	composition	866:876	composition (FT-IR, XPS) of the sorbent	866:904	Detailed characterization was performed to identify the structure (SEM, XRD) and composition (FT-IR, XPS) of the sorbent.
35926672	4	24	theme	sorbent	898:904	arg1	structure					841:849	the structure	837:849	the structure (SEM, XRD)	837:860	Detailed characterization was performed to identify the structure (SEM, XRD) and composition (FT-IR, XPS) of the sorbent.
35926672	8	25	theme	textile	1602:1608	arg1	treatment					1619:1627	textile effluent treatment	1602:1627	textile effluent treatment	1602:1627	The obtained results suggest its promising applicability at a large scale for textile effluent treatment.
35926672	0	26	theme	Microwave-assisted	0:17	arg1	synthesis					19:27	Microwave-assisted synthesis	0:27	Microwave-assisted synthesis of cross-linked chitosan-metal oxide	0:64	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	7	27	theme	field	1395:1399	arg1	conditions					1401:1410	the field conditions	1391:1410	the field conditions	1391:1410	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	7	28	theme	co-existing	1428:1438	arg1	anions/cations					1446:1459	anions/cations	1446:1459	anions/cations	1446:1459	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	7	28	theme	co-existing	1428:1438	arg1	ions					1440:1443	co-existing ions	1428:1443	co-existing ions (anions/cations)	1428:1460	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	7	28	theme	co-existing	1428:1438	arg1	regenerant					1494:1503	regenerant	1494:1503	regenerant	1494:1503	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	7	28	theme	co-existing	1428:1438	arg1	mixture					1482:1488	cocktail dyes/ions mixture	1463:1488	cocktail dyes/ions mixture	1463:1488	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	0	29	theme	cross-linked	32:43	arg1	oxide					60:64	cross-linked chitosan-metal oxide	32:64	cross-linked chitosan-metal oxide	32:64	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	1	30	theme	dye	293:295	arg1	effluent					313:320	dye(s) contaminated effluent	293:320	dye(s) contaminated effluent posing a heavy burden on the receiving water bodies	293:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	5	31	theme	methyl	933:938	arg1	MO					948:949	MO	948:949	MO	948:949	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	5	31	theme	methyl	933:938	arg1	orange					940:945	methyl orange	933:945	methyl orange (MO) dye solution	933:963	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	6	32	theme	electrostatic	1261:1273	arg1	interactions					1275:1286	electrostatic interactions	1261:1286	electrostatic interactions	1261:1286	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	1	33	theme	intense	213:219	arg1	units					237:241	the intense water-consuming units	209:241	the intense water-consuming units	209:241	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	8	34	theme	obtained	1528:1535	arg1	results					1537:1543	The obtained results	1524:1543	The obtained results	1524:1543	The obtained results suggest its promising applicability at a large scale for textile effluent treatment.
35926672	0	35	theme	effluent	133:140	arg1	matrices					142:149	unary and complex effluent matrices	115:149	unary and complex effluent matrices	115:149	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	3	36	theme	rapid	620:624	arg1	synthesis					626:634	the rapid synthesis	616:634	the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles	616:782	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	1	37	theme	water-consuming	221:235	arg1	units					237:241	the intense water-consuming units	209:241	the intense water-consuming units	209:241	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	0	38	theme	oxide	60:64	arg1	synthesis					19:27	Microwave-assisted synthesis	0:27	Microwave-assisted synthesis of cross-linked chitosan-metal oxide	0:64	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	1	39	theme	s	297:297	arg1	effluent					313:320	dye(s) contaminated effluent	293:320	dye(s) contaminated effluent posing a heavy burden on the receiving water bodies	293:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	2	40	theme	effluent	520:527	arg1	treatment					507:515	the efficient treatment	493:515	the efficient treatment of effluent	493:527	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	5	41	theme	different	988:996	arg1	2.0-12.0					1002:1009	2.0-12.0	1002:1009	2.0-12.0	1002:1009	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	5	41	theme	different	988:996	arg1	pH					998:999	different pH	988:999	different pH (2.0-12.0)	988:1010	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	1	42	theme	units	237:241	arg1	units					237:241	the intense water-consuming units	209:241	the intense water-consuming units	209:241	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	1	42	theme	units	237:241	arg1	one					202:204	one	202:204	one	202:204	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	1	42	theme	units	237:241	arg1	industries					167:176	Textile/Dyeing industries	152:176	Textile/Dyeing industries	152:176	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	0	43	theme	chitosan-metal	45:58	arg1	oxide					60:64	cross-linked chitosan-metal oxide	32:64	cross-linked chitosan-metal oxide	32:64	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	4	44	dep	structure	841:849	arg1	XRD					857:859	XRD	857:859	XRD	857:859	Detailed characterization was performed to identify the structure (SEM, XRD) and composition (FT-IR, XPS) of the sorbent.
35926672	4	44	dep	structure	841:849	arg1	SEM					852:854	SEM	852:854	SEM	852:854	Detailed characterization was performed to identify the structure (SEM, XRD) and composition (FT-IR, XPS) of the sorbent.
35926672	2	45	theme	bulk	430:433	arg1	quantity					435:442	bulk quantity	430:442	bulk quantity of adsorbent(s)	430:458	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	1	46	theme	contaminated	300:311	arg1	effluent					313:320	dye(s) contaminated effluent	293:320	dye(s) contaminated effluent posing a heavy burden on the receiving water bodies	293:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	2	47	theme	prominent	548:556	arg1	topics					558:563	the most prominent topics	539:563	the most prominent topics	539:563	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	3	48	theme	well-known	706:715	arg1	tripolyphosphate					730:745	tripolyphosphate	730:745	tripolyphosphate	730:745	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	3	48	theme	well-known	706:715	arg1	crosslinker					717:727	a well-known crosslinker	704:727	a well-known crosslinker (tripolyphosphate)	704:746	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	4	49	theme	Detailed	785:792	arg1	characterization					794:809	Detailed characterization	785:809	Detailed characterization	785:809	Detailed characterization was performed to identify the structure (SEM, XRD) and composition (FT-IR, XPS) of the sorbent.
35926672	3	50	theme	natural	669:675	arg1	biomolecule					677:687	natural biomolecule	669:687	natural biomolecule (chitosan-CS)	669:701	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	3	50	theme	natural	669:675	arg1	chitosan-CS					690:700	chitosan-CS	690:700	chitosan-CS	690:700	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	7	51	theme	ions	1440:1443	arg1	effects					1417:1423	the effects	1413:1423	the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant	1413:1503	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	2	52	theme	adsorbent	447:455	arg1	quantity					435:442	bulk quantity	430:442	bulk quantity of adsorbent(s)	430:458	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	2	52	theme	adsorbent	447:455	arg1	evaluation					478:487	further their evaluation	464:487	further their evaluation for the efficient treatment of effluent	464:527	Therefore, the identification of methods to synthesize bulk quantity of adsorbent(s) and further their evaluation for the efficient treatment of effluent is one of the most prominent topics.
35926672	1	53	theme	effluent	313:320	arg1	volume					283:288	a large volume	275:288	a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies	275:372	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	3	54	from	metal-oxide	752:762	arg1	synthesis					626:634	the rapid synthesis	616:634	the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles	616:782	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	5	55	theme	dye	952:954	arg1	solution					956:963	methyl orange (MO) dye solution	933:963	methyl orange (MO) dye solution	933:963	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	3	56	theme	C-CS	654:657	arg1	nanocomposite					639:651	nanocomposite	639:651	nanocomposite (C-CS@ZnO)	639:662	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	3	56	theme	C-CS	654:657	arg1	ZnO					659:661	C-CS@ZnO	654:661	C-CS@ZnO	654:661	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	7	57	theme	dyes/ions	1472:1480	arg1	ions					1440:1443	co-existing ions	1428:1443	co-existing ions (anions/cations)	1428:1460	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	7	57	theme	dyes/ions	1472:1480	arg1	mixture					1482:1488	cocktail dyes/ions mixture	1463:1488	cocktail dyes/ions mixture	1463:1488	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	5	58	theme	Sorption	907:914	arg1	experiments					916:926	Sorption experiments	907:926	Sorption experiments with methyl orange (MO) dye solution	907:963	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	1	59	theme	Textile/Dyeing	152:165	arg1	units					237:241	the intense water-consuming units	209:241	the intense water-consuming units	209:241	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	1	59	theme	Textile/Dyeing	152:165	arg1	one					202:204	one	202:204	one	202:204	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	1	59	theme	Textile/Dyeing	152:165	arg1	industries					167:176	Textile/Dyeing industries	152:176	Textile/Dyeing industries	152:176	Textile/Dyeing industries have been considered as one of the intense water-consuming units, resulting in the generation of a large volume of dye(s) contaminated effluent posing a heavy burden on the receiving water bodies.
35926672	8	60	theme	promising	1557:1565	arg1	applicability					1567:1579	its promising applicability	1553:1579	its promising applicability	1553:1579	The obtained results suggest its promising applicability at a large scale for textile effluent treatment.
35926672	0	61	theme	orange	91:96	arg1	removal					102:108	methyl orange dye removal	84:108	methyl orange dye removal from unary and complex effluent matrices	84:149	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	3	62	theme	nanocomposite	639:651	arg1	synthesis					626:634	the rapid synthesis	616:634	the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles	616:782	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	3	63	theme	microwave-assisted	573:590	arg1	method					592:597	microwave-assisted method	573:597	microwave-assisted method	573:597	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
35926672	7	64	theme	cocktail	1463:1470	arg1	ions					1440:1443	co-existing ions	1428:1443	co-existing ions (anions/cations)	1428:1460	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	7	64	theme	cocktail	1463:1470	arg1	mixture					1482:1488	cocktail dyes/ions mixture	1463:1488	cocktail dyes/ions mixture	1463:1488	To simulate the field conditions, the effects of co-existing ions (anions/cations), cocktail dyes/ions mixture and regenerant were also studied.
35926672	0	65	from	matrices	142:149	arg1	removal					102:108	methyl orange dye removal	84:108	methyl orange dye removal from unary and complex effluent matrices	84:149	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	0	66	theme	methyl	84:89	arg1	removal					102:108	methyl orange dye removal	84:108	methyl orange dye removal from unary and complex effluent matrices	84:149	Microwave-assisted synthesis of cross-linked chitosan-metal oxide nanocomposite for methyl orange dye removal from unary and complex effluent matrices.
35926672	5	67	theme	reaction	1050:1057	arg1	times					1059:1063	reaction times	1050:1063	reaction times (0-210 min)	1050:1075	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	5	67	theme	reaction	1050:1057	arg1	0-210 min					1066:1074	0-210 min	1066:1074	0-210 min	1066:1074	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	5	68	theme	orange	940:945	arg1	solution					956:963	methyl orange (MO) dye solution	933:963	methyl orange (MO) dye solution	933:963	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	6	69	dep	H-bonding	1247:1255	arg1	Yoshida					1239:1245	Yoshida	1239:1245	Yoshida	1239:1245	The maximum sorption capacity (185.2 mg g-1) was obtained because of the ligand-exchange, Yoshida H-bonding and electrostatic interactions and was best elucidated by Freundlich (R2 ≥ 0.99) and pseudo-second-order (R2 ≥ 1) models.
35926672	5	70	with	experiments	916:926	arg1	solution					956:963	methyl orange (MO) dye solution	933:963	methyl orange (MO) dye solution	933:963	Sorption experiments with methyl orange (MO) dye solution were carried out under different pH (2.0-12.0), dye concentrations (150-350 mg L-1), reaction times (0-210 min) and temperature (25-45 °C) to establish the adsorbent at the lab-scale.
35926672	3	71	dep	biomolecule	677:687	arg1	nanoparticles					770:782	nanoparticles	770:782	nanoparticles	770:782	Hence, microwave-assisted method was proposed for the rapid synthesis of nanocomposite (C-CS@ZnO) from natural biomolecule (chitosan-CS), a well-known crosslinker (tripolyphosphate) and metal-oxide (ZnO) nanoparticles.
37235912	10	0	theme	pancreatic	1640:1649	arg1	supplementary					1702:1714	supplementary	1702:1714	supplementary	1702:1714	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	10	0	theme	pancreatic	1640:1649	arg1	matrix					1651:1656	The extracellular pancreatic matrix	1622:1656	The extracellular pancreatic matrix obtained in this study	1622:1679	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	3	1	theme	protein-based	545:557	arg1	matrix					580:585	the protein-based tissue extracellular matrix	541:585	the protein-based tissue extracellular matrix	541:585	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	11	2	theme	matrix	1791:1796	arg1	protein					1798:1804	tissue matrix protein	1784:1804	tissue matrix protein	1784:1804	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	7	3	theme	protein	1195:1201	arg1	composition					1203:1213	the protein composition	1191:1213	the protein composition of whole pancreatic tissue	1191:1240	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	1	4	theme	mesenchymal	255:265	arg1	cells					272:276	mesenchymal stem cells	255:276	mesenchymal stem cells	255:276	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	4	5	theme	adipose	910:916	arg1	rAT-MSCs					939:946	rAT-MSCs	939:946	rAT-MSCs	939:946	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	4	5	theme	adipose	910:916	arg1	MSCs					933:936	rat adipose tissue-derived MSCs	906:936	rat adipose tissue-derived MSCs (rAT-MSCs)	906:947	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	9	6	theme	pancreatic	1505:1514	arg1	islet					1516:1520	the non-encapsulated pancreatic islet	1484:1520	the non-encapsulated pancreatic islet	1484:1520	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	3	7	theme	cells	611:615	arg1	free					603:606	free	603:606	free	603:606	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	1	8	theme	cellular	387:394	arg1	activity					396:403	their cellular activity	381:403	their cellular activity	381:403	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	1	9	theme	therapeutic	181:191	arg1	approach					193:200	a therapeutic approach	179:200	a therapeutic approach for T1DM	179:209	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	8	10	theme	pancreatic	1381:1390	arg1	tissue					1392:1397	pancreatic tissue	1381:1397	pancreatic tissue	1381:1397	The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells.
37235912	0	11	from	Islets	80:85	arg1	Investigation					0:12	Investigation	0:12	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.	0:128	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	4	12	link	tissue-derived	918:931	arg1	rAT-MSCs					939:946	rAT-MSCs	939:946	rAT-MSCs	939:946	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	4	12	link	tissue-derived	918:931	arg1	MSCs					933:936	rat adipose tissue-derived MSCs	906:936	rat adipose tissue-derived MSCs (rAT-MSCs)	906:947	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	3	13	theme	material	632:639	arg1	free					603:606	free	603:606	free	603:606	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	10	14	used	used	1694:1697	arg2	matrix					1651:1656	The extracellular pancreatic matrix	1622:1656	The extracellular pancreatic matrix obtained in this study	1622:1679	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	10	14	used	used	1694:1697	arg2	supplementary					1702:1714	supplementary	1702:1714	supplementary	1702:1714	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	5	15	theme	secretion	1003:1011	arg1	property					1013:1020	Glucose-stimulated insulin secretion property	976:1020	Glucose-stimulated insulin secretion property of the islet cells in the microbeads	976:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	12	16	from	improvement	1951:1961	arg1	viability					1975:1983	the cell viability	1966:1983	the cell viability	1966:1983	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	9	17	theme	expression	1599:1608	arg1	terms					1582:1586	terms	1582:1586	terms of insulin expression	1582:1608	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	6	18	theme	expression	1100:1109	arg1	profile					1111:1117	The gene expression profile	1091:1117	The gene expression profile of the encapsulated cells	1091:1143	The gene expression profile of the encapsulated cells was analyzed by Real-Time PCR.
37235912	4	19	theme	decellularized	740:753	arg1	matrix					780:785	the decellularized pancreatic extracellular matrix	736:785	the decellularized pancreatic extracellular matrix	736:785	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	11	20	theme	insulin	1853:1859	arg1	secretion					1861:1869	total insulin secretion	1847:1869	total insulin secretion	1847:1869	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	4	21	theme	extracellular	766:778	arg1	matrix					780:785	the decellularized pancreatic extracellular matrix	736:785	the decellularized pancreatic extracellular matrix	736:785	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	5	22	theme	Glucose-stimulated	976:993	arg1	secretion					1003:1011	Glucose-stimulated insulin secretion	976:1011	Glucose-stimulated insulin secretion property of the islet cells in the microbeads	976:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	12	23	from	efficiency	2005:2014	arg1	response					2044:2051	response	2044:2051	response to glucose stimulation	2044:2074	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	12	23	from	efficiency	2005:2014	arg1	microcapsules					2094:2106	the alginate microcapsules	2081:2106	the alginate microcapsules	2081:2106	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	0	24	from	Investigation	0:12	arg1	Islets					80:85	encapsulated Islets	67:85	encapsulated Islets of Langerhans with mesenchymal stem cells	67:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	1	25	dep	OBJECTIVE	130:138	arg1	aimed					162:166	aimed	162:166	was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity	158:403	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	0	26	theme	Langerhans	90:99	arg1	Islets					80:85	encapsulated Islets	67:85	encapsulated Islets of Langerhans with mesenchymal stem cells	67:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	1	27	theme	islets	356:361	arg1	survival					344:351	the survival	340:351	the survival of islets	340:361	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	12	28	theme	secretion	2031:2039	arg1	efficiency					2005:2014	the efficiency	2001:2014	the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules	2001:2106	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	2	29	theme	METHOD	406:411	arg1	matrix					438:443	METHOD Pancreatic extracellular matrix	406:443	METHOD Pancreatic extracellular matrix	406:443	METHOD Pancreatic extracellular matrix was decellularized using different concentrations of detergent series.
37235912	0	30	theme	mesenchymal	106:116	arg1	cells					123:127	mesenchymal stem cells	106:127	mesenchymal stem cells	106:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	0	31	theme	pancreatic	31:40	arg1	matrix					57:62	pancreatic decellularized matrix	31:62	pancreatic decellularized matrix	31:62	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	5	32	from	property	1013:1020	arg1	microbeads					1048:1057	the microbeads	1044:1057	the microbeads	1044:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	12	33	from	secretion	2031:2039	arg1	response					2044:2051	response	2044:2051	response to glucose stimulation	2044:2074	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	0	34	theme	encapsulated	67:78	arg1	Islets					80:85	encapsulated Islets	67:85	encapsulated Islets of Langerhans with mesenchymal stem cells	67:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	8	35	theme	cells	1460:1464	arg1	maintenance					1435:1445	maintenance	1435:1445	maintenance	1435:1445	The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells.
37235912	8	35	theme	cells	1460:1464	arg1	growth					1424:1429	growth	1424:1429	growth	1424:1429	The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells.
37235912	10	36	theme	cell	1765:1768	arg1	survival					1770:1777	the cell survival	1761:1777	the cell survival	1761:1777	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	1	37	theme	stem	267:270	arg1	cells					272:276	mesenchymal stem cells	255:276	mesenchymal stem cells	255:276	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	5	38	theme	islet	1029:1033	arg1	cells					1035:1039	the islet cells	1025:1039	the islet cells in the microbeads	1025:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	4	39	theme	composition	819:829	arg1	homogenization					718:731	the homogenization	714:731	the homogenization of the decellularized pancreatic extracellular matrix	714:785	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	4	39	theme	composition	819:829	arg1	analysis					795:802	the analysis	791:802	the analysis of its protein composition by LC-MS	791:838	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	7	40	theme	decellularized	1247:1260	arg1	free					1282:1285	free	1282:1285	free	1282:1285	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	7	40	theme	decellularized	1247:1260	arg1	matrix					1271:1276	the decellularized pancreas matrix	1243:1276	the decellularized pancreas matrix	1243:1276	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	12	41	from	microcapsules	2094:2106	arg1	efficiency					2005:2014	the efficiency	2001:2014	the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules	2001:2106	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	12	41	from	microcapsules	2094:2106	arg1	secretion					2031:2039	the insulin secretion	2019:2039	the insulin secretion in response to glucose stimulation from the alginate microcapsules	2019:2106	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	6	42	theme	encapsulated	1126:1137	arg1	cells					1139:1143	the encapsulated cells	1122:1143	the encapsulated cells	1122:1143	The gene expression profile of the encapsulated cells was analyzed by Real-Time PCR.
37235912	4	43	theme	pancreatic	884:893	arg1	islets					895:900	pancreatic islets	884:900	pancreatic islets	884:900	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	2	44	theme	extracellular	424:436	arg1	matrix					438:443	METHOD Pancreatic extracellular matrix	406:443	METHOD Pancreatic extracellular matrix	406:443	METHOD Pancreatic extracellular matrix was decellularized using different concentrations of detergent series.
37235912	1	45	theme	pancreatic	297:306	arg1	matrix					322:327	decellularized pancreatic extracellular matrix	282:327	decellularized pancreatic extracellular matrix	282:327	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	9	46	from	efficient	1569:1577	arg1	terms					1582:1586	terms	1582:1586	terms of insulin expression	1582:1608	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	2	47	theme	detergent	498:506	arg1	series					508:513	detergent series	498:513	detergent series	498:513	METHOD Pancreatic extracellular matrix was decellularized using different concentrations of detergent series.
37235912	10	48	theme	extracellular	1626:1638	arg1	supplementary					1702:1714	supplementary	1702:1714	supplementary	1702:1714	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	10	48	theme	extracellular	1626:1638	arg1	matrix					1651:1656	The extracellular pancreatic matrix	1622:1656	The extracellular pancreatic matrix obtained in this study	1622:1679	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	3	49	theme	histochemical	678:690	arg1	techniques					692:701	histochemical techniques	678:701	histochemical techniques	678:701	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	0	50	theme	decellularized	42:55	arg1	matrix					57:62	pancreatic decellularized matrix	31:62	pancreatic decellularized matrix	31:62	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	11	51	theme	total	1847:1851	arg1	secretion					1861:1869	total insulin secretion	1847:1869	total insulin secretion	1847:1869	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	5	52	theme	insulin	1076:1082	arg1	ELISA					1084:1088	insulin ELISA	1076:1088	insulin ELISA	1076:1088	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	3	53	theme	tissue	559:564	arg1	matrix					580:585	the protein-based tissue extracellular matrix	541:585	the protein-based tissue extracellular matrix	541:585	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	0	54	theme	matrix	57:62	arg1	effect					21:26	the effect	17:26	the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells	17:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	1	55	theme	pancreatic	232:241	arg1	islets					243:248	the pancreatic islets	228:248	the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix	228:327	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	11	56	contain	have	1884:1887	arg2	potential					1893:1901	the potential to overcome the insulin deficiency	1889:1936	the potential to overcome the insulin deficiency	1889:1936	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	11	56	contain	have	1884:1887	arg1	secretion					1861:1869	total insulin secretion	1847:1869	total insulin secretion	1847:1869	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	0	57	with	Islets	80:85	arg1	cells					123:127	mesenchymal stem cells	106:127	mesenchymal stem cells	106:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	5	58	from	microbeads	1048:1057	arg1	property					1013:1020	Glucose-stimulated insulin secretion property	976:1020	Glucose-stimulated insulin secretion property of the islet cells in the microbeads	976:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	3	59	theme	matrix	580:585	arg1	preparation					526:536	the preparation	522:536	the preparation of the protein-based tissue extracellular matrix	522:585	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	9	60	theme	encapsulated	1527:1538	arg1	efficient					1569:1577	efficient	1569:1577	efficient	1569:1577	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	9	60	theme	encapsulated	1527:1538	arg1	cells					1540:1544	the encapsulated cells	1523:1544	the encapsulated cells	1523:1544	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	7	61	theme	pancreatic	1224:1233	arg1	tissue					1235:1240	whole pancreatic tissue	1218:1240	whole pancreatic tissue	1218:1240	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	4	62	theme	rat	906:908	arg1	rAT-MSCs					939:946	rAT-MSCs	939:946	rAT-MSCs	939:946	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	4	62	theme	rat	906:908	arg1	MSCs					933:936	rat adipose tissue-derived MSCs	906:936	rat adipose tissue-derived MSCs (rAT-MSCs)	906:947	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	11	63	theme	tissue	1784:1789	arg1	protein					1798:1804	tissue matrix protein	1784:1804	tissue matrix protein	1784:1804	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	8	64	attach	derived	1368:1374	arg2	matrix					1361:1366	The protein matrix	1349:1366	The protein matrix derived from pancreatic tissue	1349:1397	The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells.
37235912	8	64	attach	derived	1368:1374	arg1	tissue					1392:1397	pancreatic tissue	1381:1397	pancreatic tissue	1381:1397	The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells.
37235912	12	65	theme	non-encapsulated	2160:2175	arg1	islets					2188:2193	the non-encapsulated pancreatic islets	2156:2193	the non-encapsulated pancreatic islets	2156:2193	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	12	66	from	number	1993:1998	arg1	viability					1975:1983	the cell viability	1966:1983	the cell viability	1966:1983	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	4	67	theme	tissue-derived	918:931	arg1	rAT-MSCs					939:946	rAT-MSCs	939:946	rAT-MSCs	939:946	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	4	67	theme	tissue-derived	918:931	arg1	MSCs					933:936	rat adipose tissue-derived MSCs	906:936	rat adipose tissue-derived MSCs (rAT-MSCs)	906:947	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	9	68	from	terms	1582:1586	arg1	efficient					1569:1577	efficient	1569:1577	efficient	1569:1577	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	9	68	from	terms	1582:1586	arg1	cells					1540:1544	the encapsulated cells	1523:1544	the encapsulated cells	1523:1544	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	11	69	theme	insulin	1919:1925	arg1	deficiency					1927:1936	the insulin deficiency	1915:1936	the insulin deficiency	1915:1936	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	10	70	dep	CONCLUSION	1611:1620	arg1	used					1694:1697	used	1694:1697	was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival	1681:1777	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	12	71	theme	alginate	2085:2092	arg1	microcapsules					2094:2106	the alginate microcapsules	2081:2106	the alginate microcapsules	2081:2106	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	12	72	theme	pancreatic	2177:2186	arg1	islets					2188:2193	the non-encapsulated pancreatic islets	2156:2193	the non-encapsulated pancreatic islets	2156:2193	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	8	73	theme	protein	1353:1359	arg1	matrix					1361:1366	The protein matrix	1349:1366	The protein matrix derived from pancreatic tissue	1349:1397	The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells.
37235912	3	74	theme	genetic	624:630	arg1	material					632:639	any genetic material	620:639	any genetic material by molecular, immunofluorescence and histochemical techniques	620:701	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	7	75	dep	RESULTS	1176:1182	arg1	composition					1203:1213	the protein composition	1191:1213	the protein composition of whole pancreatic tissue	1191:1240	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	5	76	theme	insulin	995:1001	arg1	secretion					1003:1011	Glucose-stimulated insulin secretion	976:1011	Glucose-stimulated insulin secretion property of the islet cells in the microbeads	976:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	12	77	theme	glucose	2056:2062	arg1	stimulation					2064:2074	glucose stimulation	2056:2074	glucose stimulation	2056:2074	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	4	78	theme	alginate	952:959	arg1	microcapsules					961:973	alginate microcapsules	952:973	alginate microcapsules	952:973	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	4	79	theme	matrix	780:785	arg1	homogenization					718:731	the homogenization	714:731	the homogenization of the decellularized pancreatic extracellular matrix	714:785	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	4	79	theme	matrix	780:785	arg1	analysis					795:802	the analysis	791:802	the analysis of its protein composition by LC-MS	791:838	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	9	80	theme	insulin	1591:1597	arg1	expression					1599:1608	insulin expression	1591:1608	insulin expression	1591:1608	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
37235912	7	81	theme	proteins	1310:1317	arg1	free					1282:1285	free	1282:1285	free	1282:1285	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	7	81	theme	proteins	1310:1317	arg1	matrix					1271:1276	the decellularized pancreas matrix	1243:1276	the decellularized pancreas matrix	1243:1276	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	6	82	theme	gene	1095:1098	arg1	profile					1111:1117	The gene expression profile	1091:1117	The gene expression profile of the encapsulated cells	1091:1143	The gene expression profile of the encapsulated cells was analyzed by Real-Time PCR.
37235912	0	83	from	effect	21:26	arg1	Islets					80:85	encapsulated Islets	67:85	encapsulated Islets of Langerhans with mesenchymal stem cells	67:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	7	84	theme	histone	1290:1296	arg1	proteins					1298:1305	histone proteins	1290:1305	histone proteins	1290:1305	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	7	85	theme	proteins	1298:1305	arg1	free					1282:1285	free	1282:1285	free	1282:1285	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	7	85	theme	proteins	1298:1305	arg1	matrix					1271:1276	the decellularized pancreas matrix	1243:1276	the decellularized pancreas matrix	1243:1276	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	12	86	theme	insulin	2023:2029	arg1	secretion					2031:2039	the insulin secretion	2019:2039	the insulin secretion in response to glucose stimulation from the alginate microcapsules	2019:2106	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	4	87	theme	pancreatic	755:764	arg1	matrix					780:785	the decellularized pancreatic extracellular matrix	736:785	the decellularized pancreatic extracellular matrix	736:785	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	11	88	contain	had	1819:1821	arg2	effect					1837:1842	a synergistic effect	1823:1842	a synergistic effect	1823:1842	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	11	88	contain	had	1819:1821	arg1	alginate					1810:1817	alginate	1810:1817	alginate	1810:1817	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	11	88	contain	had	1819:1821	arg1	protein					1798:1804	tissue matrix protein	1784:1804	tissue matrix protein	1784:1804	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	0	89	theme	stem	118:121	arg1	cells					123:127	mesenchymal stem cells	106:127	mesenchymal stem cells	106:127	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	6	90	theme	Real-Time	1161:1169	arg1	PCR					1171:1173	Real-Time PCR	1161:1173	Real-Time PCR	1161:1173	The gene expression profile of the encapsulated cells was analyzed by Real-Time PCR.
37235912	7	91	theme	pancreas	1262:1269	arg1	free					1282:1285	free	1282:1285	free	1282:1285	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	7	91	theme	pancreas	1262:1269	arg1	matrix					1271:1276	the decellularized pancreas matrix	1243:1276	the decellularized pancreas matrix	1243:1276	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	2	92	theme	different	470:478	arg1	concentrations					480:493	different concentrations	470:493	different concentrations of detergent series	470:513	METHOD Pancreatic extracellular matrix was decellularized using different concentrations of detergent series.
37235912	8	93	theme	islet	1454:1458	arg1	cells					1460:1464	the islet cells	1450:1464	the islet cells	1450:1464	The protein matrix derived from pancreatic tissue was shown to support the growth and maintenance of the islet cells.
37235912	0	94	theme	effect	21:26	arg1	Investigation					0:12	Investigation	0:12	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.	0:128	Investigation of the effect of pancreatic decellularized matrix on encapsulated Islets of Langerhans with mesenchymal stem cells.
37235912	5	95	theme	cells	1035:1039	arg1	property					1013:1020	Glucose-stimulated insulin secretion property	976:1020	Glucose-stimulated insulin secretion property of the islet cells in the microbeads	976:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	6	96	theme	cells	1139:1143	arg1	profile					1111:1117	The gene expression profile	1091:1117	The gene expression profile of the encapsulated cells	1091:1143	The gene expression profile of the encapsulated cells was analyzed by Real-Time PCR.
37235912	4	97	theme	protein	811:817	arg1	composition					819:829	its protein composition	807:829	its protein composition by LC-MS	807:838	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	7	98	theme	tissue	1235:1240	arg1	composition					1203:1213	the protein composition	1191:1213	the protein composition of whole pancreatic tissue	1191:1240	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	1	99	theme	decellularized	282:295	arg1	matrix					322:327	decellularized pancreatic extracellular matrix	282:327	decellularized pancreatic extracellular matrix	282:327	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	2	100	theme	series	508:513	arg1	concentrations					480:493	different concentrations	470:493	different concentrations of detergent series	470:513	METHOD Pancreatic extracellular matrix was decellularized using different concentrations of detergent series.
37235912	2	101	theme	Pancreatic	413:422	arg1	matrix					438:443	METHOD Pancreatic extracellular matrix	406:443	METHOD Pancreatic extracellular matrix	406:443	METHOD Pancreatic extracellular matrix was decellularized using different concentrations of detergent series.
37235912	7	102	theme	whole	1218:1222	arg1	tissue					1235:1240	whole pancreatic tissue	1218:1240	whole pancreatic tissue	1218:1240	RESULTS Unlike the protein composition of whole pancreatic tissue, the decellularized pancreas matrix was free of histone proteins or proteins originated from mitochondria.
37235912	1	103	theme	extracellular	308:320	arg1	matrix					322:327	decellularized pancreatic extracellular matrix	282:327	decellularized pancreatic extracellular matrix	282:327	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	5	104	from	cells	1035:1039	arg1	microbeads					1048:1057	the microbeads	1044:1057	the microbeads	1044:1057	Glucose-stimulated insulin secretion property of the islet cells in the microbeads was evaluated by insulin ELISA.
37235912	12	105	theme	cell	1970:1973	arg1	viability					1975:1983	the cell viability	1966:1983	the cell viability	1966:1983	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	1	106	with	islets	243:248	arg1	matrix					322:327	decellularized pancreatic extracellular matrix	282:327	decellularized pancreatic extracellular matrix	282:327	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	1	106	with	islets	243:248	arg1	cells					272:276	mesenchymal stem cells	255:276	mesenchymal stem cells	255:276	OBJECTIVE In this study, it was aimed to provide a therapeutic approach for T1DM by encapsulating the pancreatic islets with mesenchymal stem cells and decellularized pancreatic extracellular matrix to support the survival of islets while maintaining their cellular activity.
37235912	11	107	theme	synergistic	1825:1835	arg1	effect					1837:1842	a synergistic effect	1823:1842	a synergistic effect	1823:1842	The tissue matrix protein and alginate had a synergistic effect on total insulin secretion, which might have the potential to overcome the insulin deficiency.
37235912	4	108	theme	matrix	845:850	arg1	proteins					852:859	the matrix proteins	841:859	the matrix proteins	841:859	Following the homogenization of the decellularized pancreatic extracellular matrix and the analysis of its protein composition by LC-MS, the matrix proteins were incorporated with pancreatic islets and rat adipose tissue-derived MSCs (rAT-MSCs) in alginate microcapsules.
37235912	12	109	from	response	2044:2051	arg1	efficiency					2005:2014	the efficiency	2001:2014	the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules	2001:2106	Despite the improvement in the cell viability and the number, the efficiency of the insulin secretion in response to glucose stimulation from the alginate microcapsules did not meet the expectation when compared with the non-encapsulated pancreatic islets.
37235912	10	110	theme	alginate-based	1723:1736	arg1	microcapsule					1738:1749	the alginate-based microcapsule	1719:1749	the alginate-based microcapsule enhancing the cell survival	1719:1777	CONCLUSION The extracellular pancreatic matrix obtained in this study was directly used as supplementary in the alginate-based microcapsule enhancing the cell survival.
37235912	3	111	theme	extracellular	566:578	arg1	matrix					580:585	the protein-based tissue extracellular matrix	541:585	the protein-based tissue extracellular matrix	541:585	After the preparation of the protein-based tissue extracellular matrix was shown to be free of cells or any genetic material by molecular, immunofluorescence and histochemical techniques.
37235912	9	112	theme	non-encapsulated	1488:1503	arg1	islet					1516:1520	the non-encapsulated pancreatic islet	1484:1520	the non-encapsulated pancreatic islet	1484:1520	When compared to the non-encapsulated pancreatic islet, the encapsulated cells demonstrate to be more efficient in terms of insulin expression.
36834492	0	0	theme	Midgut	75:80	arg1	Composition					56:66	Composition	56:66	Composition	56:66	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	0	0	theme	Midgut	75:80	arg1	Structure					42:50	Structure	42:50	Structure	42:50	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	3	1	contain	contained	467:475	arg2	frames					490:495	open reading frames	477:495	open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp	477:536	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	3	1	contain	contained	467:475	arg1	cDNAs					451:455	The cDNAs	447:455	The cDNAs of SeCDAs	447:465	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	2	2	theme	Putative	324:331	arg1	CDAs					341:344	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs)	324:366	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs)	324:366	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	11	3	theme	above	1501:1505	arg1	results					1507:1513	the above results	1497:1513	the above results	1497:1513	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	11	4	theme	wall	1596:1599	arg1	layer					1606:1610	the intestinal wall cell layer	1581:1610	the intestinal wall cell layer in the midgut of S. exigua	1581:1637	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	10	5	theme	PM	1383:1384	arg1	scarce					1400:1405	scarce	1400:1405	scarce	1400:1405	Additionally, the PM structure was scarce, and the chitin microfilament structure was loose and chaotic.
36834492	10	5	theme	PM	1383:1384	arg1	structure					1386:1394	the PM structure	1379:1394	the PM structure	1379:1394	Additionally, the PM structure was scarce, and the chitin microfilament structure was loose and chaotic.
36834492	11	6	theme	layer	1606:1610	arg1	structuring					1566:1576	structuring	1566:1576	structuring	1566:1576	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	11	6	theme	layer	1606:1610	arg1	growth					1555:1560	growth	1555:1560	growth	1555:1560	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	11	7	from	midgut	1619:1624	arg1	structuring					1566:1576	structuring	1566:1576	structuring	1566:1576	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	11	7	from	midgut	1619:1624	arg1	growth					1555:1560	growth	1555:1560	growth	1555:1560	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	5	8	theme	midgut	809:814	arg1	region					795:800	the anterior region	782:800	the anterior region of the midgut	782:814	It was revealed via spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut.
36834492	3	9	theme	open	477:480	arg1	frames					490:495	open reading frames	477:495	open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp	477:536	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	11	10	from	growth	1555:1560	arg1	midgut					1619:1624	the midgut	1615:1624	the midgut of S. exigua	1615:1637	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	11	11	theme	cell	1601:1604	arg1	layer					1606:1610	the intestinal wall cell layer	1581:1610	the intestinal wall cell layer in the midgut of S. exigua	1581:1637	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	0	12	theme	Beet	85:88	arg1	Armyworm					90:97	Beet Armyworm	85:97	Beet Armyworm	85:97	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	0	12	theme	Beet	85:88	arg1	exigua					111:116	Spodoptera exigua	100:116	Spodoptera exigua	100:116	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	12	13	theme	V	1732:1732	arg1	CDAs					1734:1737	Group V CDAs	1726:1737	Group V CDAs	1726:1737	Additionally, the midgut tissue and the PM structure and composition were affected by Group V CDAs.
36834492	3	14	theme	reading	482:488	arg1	frames					490:495	open reading frames	477:495	open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp	477:536	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	3	15	theme	bp	505:506	arg1	frames					490:495	open reading frames	477:495	open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp	477:536	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	4	16	theme	amino	658:662	arg1	residues					669:676	387, 378, 385 and 383 amino acid residues	636:676	387, 378, 385 and 383 amino acid residues	636:676	The deduced protein sequences showed that SeCDAs are synthesized as preproteins of 387, 378, 385 and 383 amino acid residues, respectively.
36834492	5	17	theme	expression	728:737	arg1	analysis					739:746	spatiotemporal expression analysis	713:746	spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut	713:814	It was revealed via spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut.
36834492	8	18	theme	cells	1195:1199	arg1	layer					1170:1174	the layer	1166:1174	the layer of intestinal wall cells in the midgut	1166:1213	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	4	19	theme	residues	669:676	arg1	SeCDAs					595:600	SeCDAs	595:600	SeCDAs	595:600	The deduced protein sequences showed that SeCDAs are synthesized as preproteins of 387, 378, 385 and 383 amino acid residues, respectively.
36834492	4	19	theme	residues	669:676	arg1	preproteins					621:631	preproteins	621:631	preproteins of 387, 378, 385 and 383 amino acid residues	621:676	The deduced protein sequences showed that SeCDAs are synthesized as preproteins of 387, 378, 385 and 383 amino acid residues, respectively.
36834492	8	20	theme	RNA	1141:1143	arg1	RNAi					1159:1162	RNAi	1159:1162	RNAi	1159:1162	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	8	20	theme	RNA	1141:1143	arg1	interference					1145:1156	RNA interference	1141:1156	RNA interference (RNAi)	1141:1163	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	11	21	from	layer	1606:1610	arg1	midgut					1619:1624	the midgut	1615:1624	the midgut of S. exigua	1615:1637	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	4	22	theme	deduced	557:563	arg1	sequences					573:581	The deduced protein sequences	553:581	The deduced protein sequences	553:581	The deduced protein sequences showed that SeCDAs are synthesized as preproteins of 387, 378, 385 and 383 amino acid residues, respectively.
36834492	8	23	theme	wall	1190:1193	arg1	cells					1195:1199	intestinal wall cells	1179:1199	intestinal wall cells	1179:1199	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	0	24	theme	Armyworm	90:97	arg1	Midgut					75:80	the Midgut	71:80	the Midgut of Beet Armyworm, Spodoptera exigua	71:116	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	12	25	theme	PM	1680:1681	arg1	structure					1683:1691	the PM structure	1676:1691	the PM structure	1676:1691	Additionally, the midgut tissue and the PM structure and composition were affected by Group V CDAs.
36834492	5	26	theme	spatiotemporal	713:726	arg1	analysis					739:746	spatiotemporal expression analysis	713:746	spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut	713:814	It was revealed via spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut.
36834492	2	27	theme	beet	407:410	arg1	exigua					432:437	beet armyworm Spodoptera exigua	407:437	beet armyworm Spodoptera exigua larvae	407:444	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	8	28	theme	Group	1123:1127	arg1	CDAs					1131:1134	Group V CDAs	1123:1134	Group V CDAs	1123:1134	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	0	29	theme	V	6:6	arg1	Deacetylases					15:26	Group V Chitin Deacetylases	0:26	Group V Chitin Deacetylases	0:26	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	7	30	theme	SeCDA8	978:983	arg1	expression					953:962	the expression	949:962	the expression of SeCDA6 and SeCDA8	949:983	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	6	31	with	treatment	854:862	arg1	20E					889:891	20E	889:891	20E	889:891	The SeCDAs were down-regulated after treatment with 20-hydroxyecdysone (20E).
36834492	6	31	with	treatment	854:862	arg1	20-hydroxyecdysone					869:886	20-hydroxyecdysone	869:886	20-hydroxyecdysone (20E)	869:892	The SeCDAs were down-regulated after treatment with 20-hydroxyecdysone (20E).
36834492	8	32	dep	SeCDAV	1088:1093	arg1	sequences					1110:1118	the conserved sequences	1096:1118	the conserved sequences of Group V CDAs	1096:1134	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	5	33	theme	anterior	786:793	arg1	region					795:800	the anterior region	782:800	the anterior region of the midgut	782:814	It was revealed via spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut.
36834492	0	34	theme	Group	0:4	arg1	Deacetylases					15:26	Group V Chitin Deacetylases	0:26	Group V Chitin Deacetylases	0:26	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	8	35	theme	conserved	1100:1108	arg1	sequences					1110:1118	the conserved sequences	1096:1118	the conserved sequences of Group V CDAs	1096:1134	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	3	36	theme	1158	518:521	arg1	bp					523:524	1158 bp	518:524	1158 bp	518:524	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	8	37	theme	CDAs	1131:1134	arg1	sequences					1110:1118	the conserved sequences	1096:1118	the conserved sequences of Group V CDAs	1096:1134	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	7	38	theme	SeCDA9	1047:1052	arg1	expression					1022:1031	the expression	1018:1031	the expression of SeCDA7 and SeCDA9	1018:1052	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	1	39	theme	cuticle	259:265	arg1	structures					267:276	the cuticle structures	255:276	the cuticle structures	255:276	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	1	40	from	permeability	239:250	arg1	insects					315:321	insects	315:321	insects	315:321	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	8	41	from	layer	1170:1174	arg1	midgut					1208:1213	the midgut	1204:1213	the midgut	1204:1213	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	4	42	theme	acid	664:667	arg1	residues					669:676	387, 378, 385 and 383 amino acid residues	636:676	387, 378, 385 and 383 amino acid residues	636:676	The deduced protein sequences showed that SeCDAs are synthesized as preproteins of 387, 378, 385 and 383 amino acid residues, respectively.
36834492	4	43	theme	protein	565:571	arg1	sequences					573:581	The deduced protein sequences	553:581	The deduced protein sequences	553:581	The deduced protein sequences showed that SeCDAs are synthesized as preproteins of 387, 378, 385 and 383 amino acid residues, respectively.
36834492	0	44	theme	Chitin	8:13	arg1	Deacetylases					15:26	Group V Chitin Deacetylases	0:26	Group V Chitin Deacetylases	0:26	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	1	45	theme	structures	267:276	arg1	permeability					239:250	permeability	239:250	permeability of the cuticle structures and the peritrophic membrane (PM) in insects	239:321	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	1	45	theme	structures	267:276	arg1	properties					224:233	the mechanical properties	209:233	the mechanical properties	209:233	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	0	46	theme	Spodoptera	100:109	arg1	Armyworm					90:97	Beet Armyworm	85:97	Beet Armyworm	85:97	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	0	46	theme	Spodoptera	100:109	arg1	exigua					111:116	Spodoptera exigua	100:116	Spodoptera exigua	100:116	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	11	47	theme	V	1526:1526	arg1	CDAs					1528:1531	Group V CDAs	1520:1531	Group V CDAs	1520:1531	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	3	48	theme	bp	514:515	arg1	frames					490:495	open reading frames	477:495	open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp	477:536	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	1	49	theme	chitin	177:182	arg1	conversion					163:172	the conversion	159:172	the conversion of chitin to chitosan	159:194	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	7	50	theme	SeCDA7	1036:1041	arg1	expression					1022:1031	the expression	1018:1031	the expression of SeCDA7 and SeCDA9	1018:1052	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	8	51	theme	intestinal	1179:1188	arg1	cells					1195:1199	intestinal wall cells	1179:1199	intestinal wall cells	1179:1199	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	1	52	from	properties	224:233	arg1	insects					315:321	insects	315:321	insects	315:321	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	8	53	theme	V	1129:1129	arg1	CDAs					1131:1134	Group V CDAs	1123:1134	Group V CDAs	1123:1134	After silencing SeCDAV (the conserved sequences of Group V CDAs) via RNA interference (RNAi), the layer of intestinal wall cells in the midgut became more compact and more evenly distributed.
36834492	9	54	from	vesicles	1268:1275	arg1	midgut					1284:1289	the midgut	1280:1289	the midgut	1280:1289	The vesicles in the midgut were small and more fragmented or disappeared after SeCDAs were silenced.
36834492	12	55	theme	midgut	1658:1663	arg1	tissue					1665:1670	the midgut tissue	1654:1670	the midgut tissue	1654:1670	Additionally, the midgut tissue and the PM structure and composition were affected by Group V CDAs.
36834492	0	56	dep	Structure	42:50	arg1	the					38:40	the	38:40	the	38:40	Group V Chitin Deacetylases Influence the Structure and Composition of the Midgut of Beet Armyworm, Spodoptera exigua.
36834492	2	57	theme	exigua	432:437	arg1	larvae					439:444	beet armyworm Spodoptera exigua larvae	407:444	beet armyworm Spodoptera exigua larvae	407:444	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	1	58	theme	peritrophic	286:296	arg1	PM					308:309	PM	308:309	PM	308:309	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	1	58	theme	peritrophic	286:296	arg1	membrane					298:305	the peritrophic membrane	282:305	the peritrophic membrane (PM)	282:310	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	10	59	theme	chitin	1416:1421	arg1	loose					1451:1455	loose	1451:1455	loose	1451:1455	Additionally, the PM structure was scarce, and the chitin microfilament structure was loose and chaotic.
36834492	10	59	theme	chitin	1416:1421	arg1	structure					1437:1445	the chitin microfilament structure	1412:1445	the chitin microfilament structure	1412:1445	Additionally, the PM structure was scarce, and the chitin microfilament structure was loose and chaotic.
36834492	11	60	theme	Group	1520:1524	arg1	CDAs					1528:1531	Group V CDAs	1520:1531	Group V CDAs	1520:1531	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	3	61	theme	bp	523:524	arg1	frames					490:495	open reading frames	477:495	open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp	477:536	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	5	62	from	abundant	770:777	arg1	region					795:800	the anterior region	782:800	the anterior region of the midgut	782:814	It was revealed via spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut.
36834492	2	63	theme	Spodoptera	421:430	arg1	exigua					432:437	beet armyworm Spodoptera exigua	407:437	beet armyworm Spodoptera exigua larvae	407:444	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	1	64	theme	membrane	298:305	arg1	permeability					239:250	permeability	239:250	permeability of the cuticle structures and the peritrophic membrane (PM) in insects	239:321	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	1	64	theme	membrane	298:305	arg1	properties					224:233	the mechanical properties	209:233	the mechanical properties	209:233	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	7	65	theme	SeCDA6	967:972	arg1	expression					953:962	the expression	949:962	the expression of SeCDA6 and SeCDA8	949:983	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	7	66	theme	juvenile	918:925	arg1	JHA					943:945	JHA	943:945	JHA	943:945	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	7	66	theme	juvenile	918:925	arg1	analog					935:940	a juvenile hormone analog	916:940	a juvenile hormone analog (JHA)	916:946	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	3	67	theme	SeCDAs	460:465	arg1	cDNAs					451:455	The cDNAs	447:455	The cDNAs of SeCDAs	447:465	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	11	68	theme	S.	1629:1630	arg1	midgut					1619:1624	the midgut	1615:1624	the midgut of S. exigua	1615:1637	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	11	69	from	structuring	1566:1576	arg1	midgut					1619:1624	the midgut	1615:1624	the midgut of S. exigua	1615:1637	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	3	70	theme	bp	535:536	arg1	frames					490:495	open reading frames	477:495	open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp	477:536	The cDNAs of SeCDAs contained open reading frames of 1164 bp, 1137 bp, 1158 bp and 1152 bp, respectively.
36834492	11	71	theme	intestinal	1585:1594	arg1	layer					1606:1610	the intestinal wall cell layer	1581:1610	the intestinal wall cell layer in the midgut of S. exigua	1581:1637	It was indicated in all of the above results that Group V CDAs are essential for the growth and structuring of the intestinal wall cell layer in the midgut of S. exigua.
36834492	7	72	theme	hormone	927:933	arg1	JHA					943:945	JHA	943:945	JHA	943:945	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	7	72	theme	hormone	927:933	arg1	analog					935:940	a juvenile hormone analog	916:940	a juvenile hormone analog (JHA)	916:946	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	12	73	theme	Group	1726:1730	arg1	CDAs					1734:1737	Group V CDAs	1726:1737	Group V CDAs	1726:1737	Additionally, the midgut tissue and the PM structure and composition were affected by Group V CDAs.
36834492	2	74	dep	CDAs	341:344	arg1	SeCDAs					360:365	SeCDAs	360:365	SeCDAs	360:365	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	2	74	dep	CDAs	341:344	arg1	SeCDA6/7/8/9					346:357	SeCDA6/7/8/9	346:357	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs)	324:366	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	1	75	theme	Chitin	119:124	arg1	CDA					139:141	CDA	139:141	CDA	139:141	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	1	75	theme	Chitin	119:124	arg1	deacetylase					126:136	Chitin deacetylase	119:136	Chitin deacetylase (CDA)	119:142	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36834492	7	76	with	treatment	901:909	arg1	JHA					943:945	JHA	943:945	JHA	943:945	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	7	76	with	treatment	901:909	arg1	analog					935:940	a juvenile hormone analog	916:940	a juvenile hormone analog (JHA)	916:946	After treatment with a juvenile hormone analog (JHA), the expression of SeCDA6 and SeCDA8 was down-regulated; in contrast, the expression of SeCDA7 and SeCDA9 was up-regulated.
36834492	5	77	from	region	795:800	arg1	abundant					770:777	abundant	770:777	abundant	770:777	It was revealed via spatiotemporal expression analysis that SeCDAs were more abundant in the anterior region of the midgut.
36834492	2	78	theme	V	339:339	arg1	CDAs					341:344	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs)	324:366	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs)	324:366	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	2	79	theme	armyworm	412:419	arg1	exigua					432:437	beet armyworm Spodoptera exigua	407:437	beet armyworm Spodoptera exigua larvae	407:444	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	10	80	theme	microfilament	1423:1435	arg1	loose					1451:1455	loose	1451:1455	loose	1451:1455	Additionally, the PM structure was scarce, and the chitin microfilament structure was loose and chaotic.
36834492	10	80	theme	microfilament	1423:1435	arg1	structure					1437:1445	the chitin microfilament structure	1412:1445	the chitin microfilament structure	1412:1445	Additionally, the PM structure was scarce, and the chitin microfilament structure was loose and chaotic.
36834492	2	81	theme	Group	333:337	arg1	CDAs					341:344	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs)	324:366	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs)	324:366	Putative Group V CDAs SeCDA6/7/8/9 (SeCDAs) were identified and characterized from beet armyworm Spodoptera exigua larvae.
36834492	1	82	theme	mechanical	213:222	arg1	properties					224:233	the mechanical properties	209:233	the mechanical properties	209:233	Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects.
36791893	2	0	theme	ACSL4-mediated	337:350	arg1	inflammation					352:363	ACSL4-mediated inflammation	337:363	ACSL4-mediated inflammation	337:363	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	4	1	theme	proinflammatory	551:565	arg1	production					576:585	proinflammatory cytokine production	551:585	proinflammatory cytokine production	551:585	Knocking down ACSL4 in microglia decreased proinflammatory cytokine production.
36791893	1	2	theme	important	166:174	arg1	synthetase					114:123	Acyl-CoA synthetase	105:123	Acyl-CoA synthetase long-chain family member 4 (ACSL4)	105:158	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	2	theme	important	166:174	arg1	isozyme					176:182	an important isozyme	163:182	an important isozyme in polyunsaturated fatty acid (PUFA) metabolism	163:230	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	3	dep	synthetase	114:123	arg1	member					143:148	long-chain family member 4	125:150	Acyl-CoA synthetase long-chain family member 4 (ACSL4)	105:158	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	7	4	theme	novel	956:960	arg1	regulator					962:970	a novel regulator	954:970	a novel regulator that promotes microglia-mediated neuroinflammation by regulating VGLL4 expression and lipid metabolism	954:1073	These data revealed ACSL4 to be a novel regulator that promotes microglia-mediated neuroinflammation by regulating VGLL4 expression and lipid metabolism.
36791893	2	5	theme	-induced	282:289	arg1	inflammation					291:302	the lipopolysaccharide (LPS)-induced inflammation	254:302	the lipopolysaccharide (LPS)-induced inflammation of microglia	254:315	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	6	theme	microglia	307:315	arg1	inflammation					291:302	the lipopolysaccharide (LPS)-induced inflammation	254:302	the lipopolysaccharide (LPS)-induced inflammation of microglia	254:315	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	7	from	effects	326:332	arg1	inflammation					291:302	the lipopolysaccharide (LPS)-induced inflammation	254:302	the lipopolysaccharide (LPS)-induced inflammation of microglia	254:315	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	7	from	effects	326:332	arg1	progression					372:382	the progression	368:382	the progression of Parkinson's disease (PD)	368:410	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	4	8	theme	cytokine	567:574	arg1	production					576:585	proinflammatory cytokine production	551:585	proinflammatory cytokine production	551:585	Knocking down ACSL4 in microglia decreased proinflammatory cytokine production.
36791893	5	9	theme	lipid	726:730	arg1	composition					732:742	lipid composition	726:742	lipid composition	726:742	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	6	10	theme	down	789:792	arg1	ACSL4					794:798	knocking down ACSL4	780:798	knocking down ACSL4	780:798	In addition, knocking down ACSL4 alleviated neuroinflammation in a systemic LPS model and acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model.
36791893	3	11	theme	LPS	491:493	arg1	stimulation					495:505	LPS stimulation	491:505	LPS stimulation	491:505	In this study, we found that ACSL4 expression was increased after LPS stimulation.
36791893	6	12	theme	MPTP	909:912	arg1	model					915:919	acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model	857:919	acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model	857:919	In addition, knocking down ACSL4 alleviated neuroinflammation in a systemic LPS model and acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model.
36791893	5	13	theme	vestigial-like	619:632	arg1	member					641:646	vestigial-like family member 4	619:648	vestigial-like family member 4(VGLL4) expression	619:666	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	5	13	theme	vestigial-like	619:632	arg1	VGLL4					650:654	VGLL4	650:654	VGLL4	650:654	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	6	14	theme	knocking	780:787	arg1	ACSL4					794:798	knocking down ACSL4	780:798	knocking down ACSL4	780:798	In addition, knocking down ACSL4 alleviated neuroinflammation in a systemic LPS model and acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model.
36791893	2	15	from	role	237:240	arg1	inflammation					291:302	the lipopolysaccharide (LPS)-induced inflammation	254:302	the lipopolysaccharide (LPS)-induced inflammation of microglia	254:315	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	15	from	role	237:240	arg1	progression					372:382	the progression	368:382	the progression of Parkinson's disease (PD)	368:410	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	5	16	theme	family	634:639	arg1	member					641:646	vestigial-like family member 4	619:648	vestigial-like family member 4(VGLL4) expression	619:666	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	5	16	theme	family	634:639	arg1	VGLL4					650:654	VGLL4	650:654	VGLL4	650:654	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	1	17	theme	Acyl-CoA	105:112	arg1	synthetase					114:123	Acyl-CoA synthetase	105:123	Acyl-CoA synthetase long-chain family member 4 (ACSL4)	105:158	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	17	theme	Acyl-CoA	105:112	arg1	isozyme					176:182	an important isozyme	163:182	an important isozyme in polyunsaturated fatty acid (PUFA) metabolism	163:230	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	17	theme	Acyl-CoA	105:112	arg1	ACSL4					153:157	ACSL4	153:157	ACSL4	153:157	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	18	theme	polyunsaturated	187:201	arg1	PUFA					215:218	PUFA	215:218	PUFA	215:218	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	18	theme	polyunsaturated	187:201	arg1	acid					209:212	polyunsaturated fatty acid	187:212	polyunsaturated fatty acid (PUFA) metabolism	187:230	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	5	19	theme	member	641:646	arg1	expression					657:666	vestigial-like family member 4(VGLL4) expression	619:666	vestigial-like family member 4(VGLL4) expression	619:666	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	1	20	theme	fatty	203:207	arg1	PUFA					215:218	PUFA	215:218	PUFA	215:218	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	20	theme	fatty	203:207	arg1	acid					209:212	polyunsaturated fatty acid	187:212	polyunsaturated fatty acid (PUFA) metabolism	187:230	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	4	21	from	ACSL4	522:526	arg1	microglia					531:539	microglia	531:539	microglia	531:539	Knocking down ACSL4 in microglia decreased proinflammatory cytokine production.
36791893	4	22	theme	down	517:520	arg1	ACSL4					522:526	Knocking down ACSL4	508:526	Knocking down ACSL4 in microglia	508:539	Knocking down ACSL4 in microglia decreased proinflammatory cytokine production.
36791893	5	23	theme	signal	685:690	arg1	transduction					692:703	NF-κB signal transduction	679:703	NF-κB signal transduction	679:703	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	1	24	theme	long-chain	125:134	arg1	member					143:148	long-chain family member 4	125:150	Acyl-CoA synthetase long-chain family member 4 (ACSL4)	105:158	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	1	25	theme	acid	209:212	arg1	metabolism					221:230	polyunsaturated fatty acid (PUFA) metabolism	187:230	polyunsaturated fatty acid (PUFA) metabolism	187:230	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	0	26	theme	Microglia-Mediated	15:32	arg1	Neuroinflammation					34:50	Microglia-Mediated Neuroinflammation	15:50	Microglia-Mediated Neuroinflammation	15:50	ACSL4 Promotes Microglia-Mediated Neuroinflammation by Regulating Lipid Metabolism and VGLL4 expression.
36791893	6	27	theme	l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine	863:906	arg1	model					915:919	acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model	857:919	acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model	857:919	In addition, knocking down ACSL4 alleviated neuroinflammation in a systemic LPS model and acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model.
36791893	1	28	theme	family	136:141	arg1	member					143:148	long-chain family member 4	125:150	Acyl-CoA synthetase long-chain family member 4 (ACSL4)	105:158	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	6	29	theme	acute	857:861	arg1	model					915:919	acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model	857:919	acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model	857:919	In addition, knocking down ACSL4 alleviated neuroinflammation in a systemic LPS model and acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model.
36791893	2	30	theme	disease	399:405	arg1	progression					372:382	the progression	368:382	the progression of Parkinson's disease (PD)	368:410	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	7	31	theme	lipid	1058:1062	arg1	metabolism					1064:1073	lipid metabolism	1058:1073	lipid metabolism	1058:1073	These data revealed ACSL4 to be a novel regulator that promotes microglia-mediated neuroinflammation by regulating VGLL4 expression and lipid metabolism.
36791893	0	32	theme	Lipid	66:70	arg1	Metabolism					72:81	Lipid Metabolism	66:81	Lipid Metabolism	66:81	ACSL4 Promotes Microglia-Mediated Neuroinflammation by Regulating Lipid Metabolism and VGLL4 expression.
36791893	4	33	theme	Knocking	508:515	arg1	ACSL4					522:526	Knocking down ACSL4	508:526	Knocking down ACSL4 in microglia	508:539	Knocking down ACSL4 in microglia decreased proinflammatory cytokine production.
36791893	6	34	theme	LPS	843:845	arg1	model					847:851	a systemic LPS model	832:851	a systemic LPS model	832:851	In addition, knocking down ACSL4 alleviated neuroinflammation in a systemic LPS model and acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model.
36791893	7	35	theme	VGLL4	1037:1041	arg1	expression					1043:1052	VGLL4 expression	1037:1052	VGLL4 expression	1037:1052	These data revealed ACSL4 to be a novel regulator that promotes microglia-mediated neuroinflammation by regulating VGLL4 expression and lipid metabolism.
36791893	3	36	theme	ACSL4	454:458	arg1	expression					460:469	ACSL4 expression	454:469	ACSL4 expression	454:469	In this study, we found that ACSL4 expression was increased after LPS stimulation.
36791893	6	37	theme	systemic	834:841	arg1	model					847:851	a systemic LPS model	832:851	a systemic LPS model	832:851	In addition, knocking down ACSL4 alleviated neuroinflammation in a systemic LPS model and acute l-methyl-4-phenyl-l,2,3,6-tetrahydropyridine (MPTP) model.
36791893	7	38	theme	microglia-mediated	986:1003	arg1	neuroinflammation					1005:1021	microglia-mediated neuroinflammation	986:1021	microglia-mediated neuroinflammation	986:1021	These data revealed ACSL4 to be a novel regulator that promotes microglia-mediated neuroinflammation by regulating VGLL4 expression and lipid metabolism.
36791893	5	39	theme	NF-κB	679:683	arg1	transduction					692:703	NF-κB signal transduction	679:703	NF-κB signal transduction	679:703	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	0	40	theme	VGLL4	87:91	arg1	expression					93:102	VGLL4 expression	87:102	VGLL4 expression	87:102	ACSL4 Promotes Microglia-Mediated Neuroinflammation by Regulating Lipid Metabolism and VGLL4 expression.
36791893	2	41	theme	ACSL4	245:249	arg1	unknown					416:422	unknown	416:422	unknown	416:422	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	41	theme	ACSL4	245:249	arg1	role					237:240	The role	233:240	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia	233:315	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	41	theme	ACSL4	245:249	arg1	effects					326:332	the effects	322:332	the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD)	322:410	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	5	42	theme	LPS	750:752	arg1	stimulation					754:764	LPS stimulation	750:764	LPS stimulation	750:764	Mechanistically, ACSL4 reduced vestigial-like family member 4(VGLL4) expression to promote NF-κB signal transduction; and ACSL4 regulated lipid composition after LPS stimulation.
36791893	1	43	from	isozyme	176:182	arg1	metabolism					221:230	polyunsaturated fatty acid (PUFA) metabolism	187:230	polyunsaturated fatty acid (PUFA) metabolism	187:230	Acyl-CoA synthetase long-chain family member 4 (ACSL4) is an important isozyme in polyunsaturated fatty acid (PUFA) metabolism.
36791893	2	44	theme	inflammation	352:363	arg1	unknown					416:422	unknown	416:422	unknown	416:422	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	44	theme	inflammation	352:363	arg1	role					237:240	The role	233:240	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia	233:315	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
36791893	2	44	theme	inflammation	352:363	arg1	effects					326:332	the effects	322:332	the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD)	322:410	The role of ACSL4 in the lipopolysaccharide (LPS)-induced inflammation of microglia, and the effects of ACSL4-mediated inflammation on the progression of Parkinson's disease (PD) are unknown.
35638781	20	0	theme	products	3001:3008	arg1	combinations					2976:2987	specific combinations	2967:2987	specific combinations of cls gene products	2967:3008	The macrophage phenotypes are not directly attributable to CL production but are caused by deleting specific combinations of cls gene products.
35638781	10	1	theme	dehydrogenase	1465:1477	arg1	dehydrogenase					1465:1477	lactate dehydrogenase	1457:1477	lactate dehydrogenase	1457:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	1	theme	dehydrogenase	1465:1477	arg1	amounts					1398:1404	reduced amounts	1390:1404	reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase	1390:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	1	theme	dehydrogenase	1465:1477	arg1	IL-1β					1438:1442	IL-1β	1438:1442	IL-1β	1438:1442	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	1	theme	dehydrogenase	1465:1477	arg1	IL-18					1446:1450	IL-18	1446:1450	IL-18	1446:1450	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	1	theme	dehydrogenase	1465:1477	arg1	beta					1432:1435	secreted interleukin-1 beta	1409:1435	secreted interleukin-1 beta (IL-1β)	1409:1443	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	3	2	theme	CL	519:520	arg1	molecules					522:530	mitochondrial CL molecules	505:530	mitochondrial CL molecules	505:530	S. Typhimurium regulates the levels of acidic glycerophospholipids known as cardiolipins (CL) within the OM, and mitochondrial CL molecules can prime and activate host inflammasomes.
35638781	16	3	theme	receptor	2338:2345	arg1	activation					2314:2323	LPS activation	2310:2323	LPS activation of Toll-like receptor 4 (Tlr4)	2310:2354	Purified bacterial CL molecules also influence LPS activation of Toll-like receptor 4 (Tlr4).
35638781	3	4	theme	host	555:558	arg1	inflammasomes					560:572	host inflammasomes	555:572	host inflammasomes	555:572	S. Typhimurium regulates the levels of acidic glycerophospholipids known as cardiolipins (CL) within the OM, and mitochondrial CL molecules can prime and activate host inflammasomes.
35638781	19	5	from	survival	2706:2713	arg1	macrophages					2718:2728	macrophages	2718:2728	macrophages	2718:2728	This study supports that the genes encoding the CL synthases work coordinately to promote intracellular survival in macrophages and to activate the inflammasome but do not influence inflammatory cytokine production downstream of Tlr4 or virulence in C57BL/6J mice.
35638781	4	6	theme	inflammasome	670:681	arg1	activation					683:692	inflammasome activation	670:692	inflammasome activation	670:692	However, the contribution of S. Typhimurium's CL biosynthesis genes to intracellular survival, inflammasome activation, and pathogenesis had not been examined.
35638781	13	7	theme	CL	1713:1714	arg1	synthase					1716:1723	CL synthase	1713:1723	the three CL synthase genes	1703:1729	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	9	8	theme	oral	1186:1189	arg1	infection					1204:1212	oral and systemic infection	1186:1212	oral and systemic infection for C57BL/6J mice	1186:1230	Despite being devoid of CL molecules, ΔclsABC mutants were highly virulent during oral and systemic infection for C57BL/6J mice.
35638781	15	9	theme	CL	2137:2138	arg1	molecules					2140:2148	Mitochondrial CL molecules	2123:2148	Mitochondrial CL molecules	2123:2148	Mitochondrial CL molecules activate the inflammasome and its effector caspase-1, which initiates an inflammatory process called pyroptosis.
35638781	18	10	theme	genes	2526:2530	arg1	contribution					2478:2489	the contribution	2474:2489	the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation	2474:2579	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	6	11	theme	double	791:796	arg1	mutants					810:816	Single, double, and triple mutants	783:816	Single, double, and triple mutants	783:816	Single, double, and triple mutants were constructed.
35638781	2	12	theme	membrane	338:345	arg1	composition					313:323	the lipid composition	303:323	the lipid composition of the outer membrane (OM)	303:350	The vacuolar environment prompts the bacterium to regulate the lipid composition of the outer membrane (OM), and this influences host inflammation.
35638781	18	13	theme	CL	2514:2515	arg1	genes					2526:2530	the three bacterial CL synthase genes	2494:2530	the three bacterial CL synthase genes (cls)	2494:2536	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	18	13	theme	CL	2514:2515	arg1	cls					2533:2535	cls	2533:2535	cls	2533:2535	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	5	14	theme	S.	735:736	arg1	synthases					772:780	three CL synthases	763:780	three CL synthases	763:780	S. Typhimurium genes encode three CL synthases.
35638781	5	14	theme	S.	735:736	arg1	genes					750:754	S. Typhimurium genes	735:754	S. Typhimurium genes	735:754	S. Typhimurium genes encode three CL synthases.
35638781	20	15	theme	CL	2926:2927	arg1	production					2929:2938	CL production	2926:2938	CL production	2926:2938	The macrophage phenotypes are not directly attributable to CL production but are caused by deleting specific combinations of cls gene products.
35638781	19	16	theme	inflammatory	2784:2795	arg1	production					2806:2815	inflammatory cytokine production	2784:2815	inflammatory cytokine production	2784:2815	This study supports that the genes encoding the CL synthases work coordinately to promote intracellular survival in macrophages and to activate the inflammasome but do not influence inflammatory cytokine production downstream of Tlr4 or virulence in C57BL/6J mice.
35638781	13	17	from	virulence	1891:1899	arg1	mice					1904:1907	mice	1904:1907	mice	1904:1907	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	16	18	theme	bacterial	2272:2280	arg1	molecules					2285:2293	Purified bacterial CL molecules	2263:2293	Purified bacterial CL molecules	2263:2293	Purified bacterial CL molecules also influence LPS activation of Toll-like receptor 4 (Tlr4).
35638781	7	19	theme	CL	982:983	arg1	production					985:994	CL production	982:994	CL production in stationary phase	982:1014	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	14	20	theme	membrane	2091:2098	arg1	composition					2066:2076	the cardiolipin (CL) and lipopolysaccharide (LPS) composition	2016:2076	the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection	2016:2120	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	0	21	theme	Typhimurium	80:90	arg1	Pathogenesis					92:103	Salmonella enterica Serovar Typhimurium Pathogenesis	52:103	Salmonella enterica Serovar Typhimurium Pathogenesis in C57BL/6J Mice	52:120	Cardiolipin Biosynthesis Genes Are Not Required for Salmonella enterica Serovar Typhimurium Pathogenesis in C57BL/6J Mice.
35638781	10	22	theme	ΔclsBC	1333:1338	arg1	mutants					1353:1359	ΔclsAB, ΔclsBC, and ΔclsABC mutants	1325:1359	ΔclsAB, ΔclsBC, and ΔclsABC mutants	1325:1359	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	13	23	theme	mouse	1849:1853	arg1	macrophages					1855:1865	C57BL/6J mouse macrophages	1840:1865	C57BL/6J mouse macrophages	1840:1865	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	10	24	theme	beta	1432:1435	arg1	dehydrogenase					1465:1477	lactate dehydrogenase	1457:1477	lactate dehydrogenase	1457:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	24	theme	beta	1432:1435	arg1	amounts					1398:1404	reduced amounts	1390:1404	reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase	1390:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	24	theme	beta	1432:1435	arg1	IL-1β					1438:1442	IL-1β	1438:1442	IL-1β	1438:1442	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	24	theme	beta	1432:1435	arg1	IL-18					1446:1450	IL-18	1446:1450	IL-18	1446:1450	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	24	theme	beta	1432:1435	arg1	beta					1432:1435	secreted interleukin-1 beta	1409:1435	secreted interleukin-1 beta (IL-1β)	1409:1443	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	14	25	theme	pathogenic	1966:1975	arg1	IMPORTANCE					1910:1919	IMPORTANCE	1910:1919	IMPORTANCE Salmonella enterica serovar Typhimurium	1910:1959	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	14	25	theme	pathogenic	1966:1975	arg1	bacterium					1991:1999	a pathogenic Gram-negative bacterium	1964:1999	a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection	1964:2120	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	10	26	theme	secreted	1409:1416	arg1	IL-1β					1438:1442	IL-1β	1438:1442	IL-1β	1438:1442	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	26	theme	secreted	1409:1416	arg1	beta					1432:1435	secreted interleukin-1 beta	1409:1435	secreted interleukin-1 beta (IL-1β)	1409:1443	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	13	27	theme	intracellular	1814:1826	arg1	survival					1828:1835	intracellular survival	1814:1835	intracellular survival	1814:1835	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	3	28	theme	glycerophospholipids	438:457	arg1	levels					421:426	the levels	417:426	the levels of acidic glycerophospholipids known as cardiolipins (CL) within the OM	417:498	S. Typhimurium regulates the levels of acidic glycerophospholipids known as cardiolipins (CL) within the OM, and mitochondrial CL molecules can prime and activate host inflammasomes.
35638781	0	29	theme	Cardiolipin	0:10	arg1	Genes					25:29	Cardiolipin Biosynthesis Genes	0:29	Cardiolipin Biosynthesis Genes	0:29	Cardiolipin Biosynthesis Genes Are Not Required for Salmonella enterica Serovar Typhimurium Pathogenesis in C57BL/6J Mice.
35638781	7	30	theme	logarithmic	931:941	arg1	growth					943:948	logarithmic growth	931:948	logarithmic growth	931:948	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	20	31	theme	specific	2967:2974	arg1	combinations					2976:2987	specific combinations	2967:2987	specific combinations of cls gene products	2967:3008	The macrophage phenotypes are not directly attributable to CL production but are caused by deleting specific combinations of cls gene products.
35638781	14	32	theme	lipopolysaccharide	2041:2058	arg1	composition					2066:2076	the cardiolipin (CL) and lipopolysaccharide (LPS) composition	2016:2076	the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection	2016:2120	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	6	33	theme	triple	803:808	arg1	mutants					810:816	Single, double, and triple mutants	783:816	Single, double, and triple mutants	783:816	Single, double, and triple mutants were constructed.
35638781	10	34	theme	ΔclsAC	1274:1279	arg1	mutants					1281:1287	ΔclsAC mutants	1274:1287	ΔclsAC mutants	1274:1287	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	15	35	theme	inflammatory	2223:2234	arg1	process					2236:2242	an inflammatory process	2220:2242	an inflammatory process called pyroptosis	2220:2260	Mitochondrial CL molecules activate the inflammasome and its effector caspase-1, which initiates an inflammatory process called pyroptosis.
35638781	17	36	theme	macrophage	2387:2396	arg1	vacuoles					2398:2405	macrophage vacuoles	2387:2405	macrophage vacuoles	2387:2405	S. Typhimurium resides within macrophage vacuoles and activates Tlr4 and the inflammasome during infection.
35638781	10	37	theme	lactate	1457:1463	arg1	dehydrogenase					1465:1477	lactate dehydrogenase	1457:1477	lactate dehydrogenase	1457:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	9	38	theme	CL	1128:1129	arg1	molecules					1131:1139	CL molecules	1128:1139	CL molecules	1128:1139	Despite being devoid of CL molecules, ΔclsABC mutants were highly virulent during oral and systemic infection for C57BL/6J mice.
35638781	10	39	theme	wild	1306:1309	arg1	type					1311:1314	the wild type	1302:1314	the wild type	1302:1314	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	13	40	dep	S.	1771:1772	arg1	Typhimurium					1774:1784	Typhimurium	1774:1784	Typhimurium	1774:1784	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	13	41	theme	synthase	1716:1723	arg1	genes					1725:1729	the three CL synthase genes	1703:1729	the three CL synthase genes	1703:1729	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	13	41	theme	synthase	1716:1723	arg1	dispensable					1875:1885	dispensable	1875:1885	dispensable	1875:1885	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	13	42	from	activation	1799:1808	arg1	macrophages					1855:1865	C57BL/6J mouse macrophages	1840:1865	C57BL/6J mouse macrophages	1840:1865	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	4	43	theme	CL	621:622	arg1	genes					637:641	S. Typhimurium's CL biosynthesis genes	604:641	S. Typhimurium's CL biosynthesis genes	604:641	However, the contribution of S. Typhimurium's CL biosynthesis genes to intracellular survival, inflammasome activation, and pathogenesis had not been examined.
35638781	3	44	theme	mitochondrial	505:517	arg1	molecules					522:530	mitochondrial CL molecules	505:530	mitochondrial CL molecules	505:530	S. Typhimurium regulates the levels of acidic glycerophospholipids known as cardiolipins (CL) within the OM, and mitochondrial CL molecules can prime and activate host inflammasomes.
35638781	20	45	theme	gene	2996:2999	arg1	products					3001:3008	cls gene products	2992:3008	cls gene products	2992:3008	The macrophage phenotypes are not directly attributable to CL production but are caused by deleting specific combinations of cls gene products.
35638781	7	46	theme	primary	885:891	arg1	synthase					896:903	the primary CL synthase	881:903	the primary CL synthase for S. Typhimurium	881:922	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	7	46	theme	primary	885:891	arg1	ClsA					873:876	ClsA	873:876	ClsA	873:876	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	19	47	theme	cytokine	2797:2804	arg1	production					2806:2815	inflammatory cytokine production	2784:2815	inflammatory cytokine production	2784:2815	This study supports that the genes encoding the CL synthases work coordinately to promote intracellular survival in macrophages and to activate the inflammasome but do not influence inflammatory cytokine production downstream of Tlr4 or virulence in C57BL/6J mice.
35638781	19	48	theme	C57BL/6J	2852:2859	arg1	mice					2861:2864	C57BL/6J mice	2852:2864	C57BL/6J mice	2852:2864	This study supports that the genes encoding the CL synthases work coordinately to promote intracellular survival in macrophages and to activate the inflammasome but do not influence inflammatory cytokine production downstream of Tlr4 or virulence in C57BL/6J mice.
35638781	16	49	theme	Toll-like	2328:2336	arg1	receptor					2338:2345	Toll-like receptor 4	2328:2347	Toll-like receptor 4 (Tlr4)	2328:2354	Purified bacterial CL molecules also influence LPS activation of Toll-like receptor 4 (Tlr4).
35638781	16	49	theme	Toll-like	2328:2336	arg1	Tlr4					2350:2353	Tlr4	2350:2353	Tlr4	2350:2353	Purified bacterial CL molecules also influence LPS activation of Toll-like receptor 4 (Tlr4).
35638781	2	50	theme	lipid	307:311	arg1	composition					313:323	the lipid composition	303:323	the lipid composition of the outer membrane (OM)	303:350	The vacuolar environment prompts the bacterium to regulate the lipid composition of the outer membrane (OM), and this influences host inflammation.
35638781	15	51	theme	Mitochondrial	2123:2135	arg1	molecules					2140:2148	Mitochondrial CL molecules	2123:2148	Mitochondrial CL molecules	2123:2148	Mitochondrial CL molecules activate the inflammasome and its effector caspase-1, which initiates an inflammatory process called pyroptosis.
35638781	7	52	from	production	985:994	arg1	phase					1010:1014	stationary phase	999:1014	stationary phase	999:1014	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	11	53	theme	inflammasome	1590:1601	arg1	activation					1603:1612	inflammasome activation	1590:1612	inflammasome activation	1590:1612	Hence, when clsA and clsC are deleted, clsB is necessary and sufficient to promote intracellular survival and inflammasome activation.
35638781	14	54	theme	outer	2085:2089	arg1	OM					2101:2102	OM	2101:2102	OM	2101:2102	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	14	54	theme	outer	2085:2089	arg1	membrane					2091:2098	the outer membrane	2081:2098	the outer membrane (OM)	2081:2103	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	18	55	theme	synthase	2517:2524	arg1	genes					2526:2530	the three bacterial CL synthase genes	2494:2530	the three bacterial CL synthase genes (cls)	2494:2536	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	18	55	theme	synthase	2517:2524	arg1	cls					2533:2535	cls	2533:2535	cls	2533:2535	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	9	56	theme	systemic	1195:1202	arg1	infection					1204:1212	oral and systemic infection	1186:1212	oral and systemic infection for C57BL/6J mice	1186:1230	Despite being devoid of CL molecules, ΔclsABC mutants were highly virulent during oral and systemic infection for C57BL/6J mice.
35638781	10	57	theme	ΔclsABC	1345:1351	arg1	mutants					1353:1359	ΔclsAB, ΔclsBC, and ΔclsABC mutants	1325:1359	ΔclsAB, ΔclsBC, and ΔclsABC mutants	1325:1359	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	2	58	theme	outer	332:336	arg1	OM					348:349	OM	348:349	OM	348:349	The vacuolar environment prompts the bacterium to regulate the lipid composition of the outer membrane (OM), and this influences host inflammation.
35638781	2	58	theme	outer	332:336	arg1	membrane					338:345	the outer membrane	328:345	the outer membrane (OM)	328:350	The vacuolar environment prompts the bacterium to regulate the lipid composition of the outer membrane (OM), and this influences host inflammation.
35638781	7	59	theme	other	847:851	arg1	Enterobacteriaceae					853:870	other Enterobacteriaceae	847:870	other Enterobacteriaceae	847:870	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	0	60	from	Pathogenesis	92:103	arg1	Mice					117:120	C57BL/6J Mice	108:120	C57BL/6J Mice	108:120	Cardiolipin Biosynthesis Genes Are Not Required for Salmonella enterica Serovar Typhimurium Pathogenesis in C57BL/6J Mice.
35638781	15	61	dep	inflammasome	2163:2174	arg1	the					2159:2161	the	2159:2161	the	2159:2161	Mitochondrial CL molecules activate the inflammasome and its effector caspase-1, which initiates an inflammatory process called pyroptosis.
35638781	18	62	theme	microbial	2541:2549	arg1	pathogenesis					2551:2562	microbial pathogenesis	2541:2562	microbial pathogenesis	2541:2562	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	9	63	theme	C57BL/6J	1218:1225	arg1	mice					1227:1230	C57BL/6J mice	1218:1230	C57BL/6J mice	1218:1230	Despite being devoid of CL molecules, ΔclsABC mutants were highly virulent during oral and systemic infection for C57BL/6J mice.
35638781	4	64	theme	intracellular	646:658	arg1	survival					660:667	intracellular survival	646:667	intracellular survival	646:667	However, the contribution of S. Typhimurium's CL biosynthesis genes to intracellular survival, inflammasome activation, and pathogenesis had not been examined.
35638781	5	65	theme	Typhimurium	738:748	arg1	synthases					772:780	three CL synthases	763:780	three CL synthases	763:780	S. Typhimurium genes encode three CL synthases.
35638781	5	65	theme	Typhimurium	738:748	arg1	genes					750:754	S. Typhimurium genes	735:754	S. Typhimurium genes	735:754	S. Typhimurium genes encode three CL synthases.
35638781	2	66	theme	vacuolar	248:255	arg1	environment					257:267	The vacuolar environment	244:267	The vacuolar environment	244:267	The vacuolar environment prompts the bacterium to regulate the lipid composition of the outer membrane (OM), and this influences host inflammation.
35638781	15	67	theme	effector	2184:2191	arg1	caspase-1					2193:2201	its effector caspase-1	2180:2201	its effector caspase-1	2180:2201	Mitochondrial CL molecules activate the inflammasome and its effector caspase-1, which initiates an inflammatory process called pyroptosis.
35638781	16	68	theme	Purified	2263:2270	arg1	molecules					2285:2293	Purified bacterial CL molecules	2263:2293	Purified bacterial CL molecules	2263:2293	Purified bacterial CL molecules also influence LPS activation of Toll-like receptor 4 (Tlr4).
35638781	14	69	dep	IMPORTANCE	1910:1919	arg1	Typhimurium					1949:1959	Salmonella enterica serovar Typhimurium	1921:1959	IMPORTANCE Salmonella enterica serovar Typhimurium	1910:1959	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	20	70	theme	macrophage	2871:2880	arg1	attributable					2910:2921	attributable	2910:2921	attributable	2910:2921	The macrophage phenotypes are not directly attributable to CL production but are caused by deleting specific combinations of cls gene products.
35638781	20	70	theme	macrophage	2871:2880	arg1	phenotypes					2882:2891	The macrophage phenotypes	2867:2891	The macrophage phenotypes	2867:2891	The macrophage phenotypes are not directly attributable to CL production but are caused by deleting specific combinations of cls gene products.
35638781	16	71	theme	CL	2282:2283	arg1	molecules					2285:2293	Purified bacterial CL molecules	2263:2293	Purified bacterial CL molecules	2263:2293	Purified bacterial CL molecules also influence LPS activation of Toll-like receptor 4 (Tlr4).
35638781	5	72	theme	CL	769:770	arg1	synthases					772:780	three CL synthases	763:780	three CL synthases	763:780	S. Typhimurium genes encode three CL synthases.
35638781	5	72	theme	CL	769:770	arg1	genes					750:754	S. Typhimurium genes	735:754	S. Typhimurium genes	735:754	S. Typhimurium genes encode three CL synthases.
35638781	7	73	theme	stationary	999:1008	arg1	phase					1010:1014	stationary phase	999:1014	stationary phase	999:1014	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	1	74	theme	intracellular	169:181	arg1	Typhimurium					151:161	Salmonella enterica serovar Typhimurium	123:161	Salmonella enterica serovar Typhimurium	123:161	Salmonella enterica serovar Typhimurium is an intracellular pathogen that parasitizes macrophages from within a vacuole.
35638781	1	74	theme	intracellular	169:181	arg1	pathogen					183:190	an intracellular pathogen	166:190	an intracellular pathogen that parasitizes macrophages from within a vacuole	166:241	Salmonella enterica serovar Typhimurium is an intracellular pathogen that parasitizes macrophages from within a vacuole.
35638781	10	75	theme	reduced	1390:1396	arg1	dehydrogenase					1465:1477	lactate dehydrogenase	1457:1477	lactate dehydrogenase	1457:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	75	theme	reduced	1390:1396	arg1	amounts					1398:1404	reduced amounts	1390:1404	reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase	1390:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	75	theme	reduced	1390:1396	arg1	IL-1β					1438:1442	IL-1β	1438:1442	IL-1β	1438:1442	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	75	theme	reduced	1390:1396	arg1	IL-18					1446:1450	IL-18	1446:1450	IL-18	1446:1450	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	75	theme	reduced	1390:1396	arg1	beta					1432:1435	secreted interleukin-1 beta	1409:1435	secreted interleukin-1 beta (IL-1β)	1409:1443	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	13	76	theme	C57BL/6J	1840:1847	arg1	macrophages					1855:1865	C57BL/6J mouse macrophages	1840:1865	C57BL/6J mouse macrophages	1840:1865	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	0	77	theme	C57BL/6J	108:115	arg1	Mice					117:120	C57BL/6J Mice	108:120	C57BL/6J Mice	108:120	Cardiolipin Biosynthesis Genes Are Not Required for Salmonella enterica Serovar Typhimurium Pathogenesis in C57BL/6J Mice.
35638781	9	78	theme	molecules	1131:1139	arg1	devoid					1118:1123	devoid	1118:1123	devoid	1118:1123	Despite being devoid of CL molecules, ΔclsABC mutants were highly virulent during oral and systemic infection for C57BL/6J mice.
35638781	13	79	theme	inflammasome	1786:1797	arg1	activation					1799:1808	S. Typhimurium inflammasome activation	1771:1808	S. Typhimurium inflammasome activation	1771:1808	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	13	80	from	survival	1828:1835	arg1	macrophages					1855:1865	C57BL/6J mouse macrophages	1840:1865	C57BL/6J mouse macrophages	1840:1865	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	10	81	theme	interleukin-1	1418:1430	arg1	IL-1β					1438:1442	IL-1β	1438:1442	IL-1β	1438:1442	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	81	theme	interleukin-1	1418:1430	arg1	beta					1432:1435	secreted interleukin-1 beta	1409:1435	secreted interleukin-1 beta (IL-1β)	1409:1443	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	0	82	theme	Biosynthesis	12:23	arg1	Genes					25:29	Cardiolipin Biosynthesis Genes	0:29	Cardiolipin Biosynthesis Genes	0:29	Cardiolipin Biosynthesis Genes Are Not Required for Salmonella enterica Serovar Typhimurium Pathogenesis in C57BL/6J Mice.
35638781	18	83	theme	bacterial	2504:2512	arg1	genes					2526:2530	the three bacterial CL synthase genes	2494:2530	the three bacterial CL synthase genes (cls)	2494:2536	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	18	83	theme	bacterial	2504:2512	arg1	cls					2533:2535	cls	2533:2535	cls	2533:2535	However, the contribution of the three bacterial CL synthase genes (cls) to microbial pathogenesis and inflammation had not been tested.
35638781	8	84	theme	CL	1076:1077	arg1	content					1079:1085	the CL content	1072:1085	the CL content of the envelope	1072:1101	It was necessary to delete all three genes to diminish the CL content of the envelope.
35638781	14	85	theme	Gram-negative	1977:1989	arg1	IMPORTANCE					1910:1919	IMPORTANCE	1910:1919	IMPORTANCE Salmonella enterica serovar Typhimurium	1910:1959	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	14	85	theme	Gram-negative	1977:1989	arg1	bacterium					1991:1999	a pathogenic Gram-negative bacterium	1964:1999	a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection	1964:2120	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	13	86	theme	S.	1771:1772	arg1	activation					1799:1808	S. Typhimurium inflammasome activation	1771:1808	S. Typhimurium inflammasome activation	1771:1808	Therefore, the three CL synthase genes cooperatively and redundantly influence S. Typhimurium inflammasome activation and intracellular survival in C57BL/6J mouse macrophages but are dispensable for virulence in mice.
35638781	3	87	theme	acidic	431:436	arg1	glycerophospholipids					438:457	acidic glycerophospholipids	431:457	acidic glycerophospholipids known as cardiolipins (CL) within the OM	431:498	S. Typhimurium regulates the levels of acidic glycerophospholipids known as cardiolipins (CL) within the OM, and mitochondrial CL molecules can prime and activate host inflammasomes.
35638781	6	88	theme	Single	783:788	arg1	mutants					810:816	Single, double, and triple mutants	783:816	Single, double, and triple mutants	783:816	Single, double, and triple mutants were constructed.
35638781	8	89	theme	envelope	1094:1101	arg1	content					1079:1085	the CL content	1072:1085	the CL content of the envelope	1072:1101	It was necessary to delete all three genes to diminish the CL content of the envelope.
35638781	19	90	theme	CL	2650:2651	arg1	synthases					2653:2661	the CL synthases	2646:2661	the CL synthases	2646:2661	This study supports that the genes encoding the CL synthases work coordinately to promote intracellular survival in macrophages and to activate the inflammasome but do not influence inflammatory cytokine production downstream of Tlr4 or virulence in C57BL/6J mice.
35638781	16	91	theme	LPS	2310:2312	arg1	activation					2314:2323	LPS activation	2310:2323	LPS activation of Toll-like receptor 4 (Tlr4)	2310:2354	Purified bacterial CL molecules also influence LPS activation of Toll-like receptor 4 (Tlr4).
35638781	14	92	theme	cardiolipin	2020:2030	arg1	composition					2066:2076	the cardiolipin (CL) and lipopolysaccharide (LPS) composition	2016:2076	the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection	2016:2120	IMPORTANCE Salmonella enterica serovar Typhimurium is a pathogenic Gram-negative bacterium that regulates the cardiolipin (CL) and lipopolysaccharide (LPS) composition of the outer membrane (OM) during infection.
35638781	19	93	theme	intracellular	2692:2704	arg1	survival					2706:2713	intracellular survival	2692:2713	intracellular survival in macrophages	2692:2728	This study supports that the genes encoding the CL synthases work coordinately to promote intracellular survival in macrophages and to activate the inflammasome but do not influence inflammatory cytokine production downstream of Tlr4 or virulence in C57BL/6J mice.
35638781	10	94	theme	IL-18	1446:1450	arg1	dehydrogenase					1465:1477	lactate dehydrogenase	1457:1477	lactate dehydrogenase	1457:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	94	theme	IL-18	1446:1450	arg1	amounts					1398:1404	reduced amounts	1390:1404	reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase	1390:1477	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	94	theme	IL-18	1446:1450	arg1	IL-1β					1438:1442	IL-1β	1438:1442	IL-1β	1438:1442	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	94	theme	IL-18	1446:1450	arg1	IL-18					1446:1450	IL-18	1446:1450	IL-18	1446:1450	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	94	theme	IL-18	1446:1450	arg1	beta					1432:1435	secreted interleukin-1 beta	1409:1435	secreted interleukin-1 beta (IL-1β)	1409:1443	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	10	95	theme	ΔclsAB	1325:1330	arg1	mutants					1353:1359	ΔclsAB, ΔclsBC, and ΔclsABC mutants	1325:1359	ΔclsAB, ΔclsBC, and ΔclsABC mutants	1325:1359	In macrophages, ΔclsA, ΔclsB, ΔclsC, and ΔclsAC mutants behaved like the wild type, whereas ΔclsAB, ΔclsBC, and ΔclsABC mutants were attenuated and elicited reduced amounts of secreted interleukin-1 beta (IL-1β), IL-18, and lactate dehydrogenase.
35638781	20	96	theme	cls	2992:2994	arg1	products					3001:3008	cls gene products	2992:3008	cls gene products	2992:3008	The macrophage phenotypes are not directly attributable to CL production but are caused by deleting specific combinations of cls gene products.
35638781	4	97	theme	biosynthesis	624:635	arg1	genes					637:641	S. Typhimurium's CL biosynthesis genes	604:641	S. Typhimurium's CL biosynthesis genes	604:641	However, the contribution of S. Typhimurium's CL biosynthesis genes to intracellular survival, inflammasome activation, and pathogenesis had not been examined.
35638781	11	98	theme	intracellular	1563:1575	arg1	survival					1577:1584	intracellular survival	1563:1584	intracellular survival	1563:1584	Hence, when clsA and clsC are deleted, clsB is necessary and sufficient to promote intracellular survival and inflammasome activation.
35638781	9	99	theme	ΔclsABC	1142:1148	arg1	mutants					1150:1156	ΔclsABC mutants	1142:1156	ΔclsABC mutants	1142:1156	Despite being devoid of CL molecules, ΔclsABC mutants were highly virulent during oral and systemic infection for C57BL/6J mice.
35638781	2	100	theme	host	373:376	arg1	inflammation					378:389	host inflammation	373:389	host inflammation	373:389	The vacuolar environment prompts the bacterium to regulate the lipid composition of the outer membrane (OM), and this influences host inflammation.
35638781	7	101	theme	CL	893:894	arg1	synthase					896:903	the primary CL synthase	881:903	the primary CL synthase for S. Typhimurium	881:922	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
35638781	7	101	theme	CL	893:894	arg1	ClsA					873:876	ClsA	873:876	ClsA	873:876	Similar to other Enterobacteriaceae, ClsA is the primary CL synthase for S. Typhimurium during logarithmic growth, while ClsB and ClsC contribute CL production in stationary phase.
37187351	2	0	theme	lipopolysaccharide	511:528	arg1	challenge					536:544	a lipopolysaccharide (LPS) challenge	509:544	a lipopolysaccharide (LPS) challenge	509:544	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	10	1	theme	oxylipins	1679:1687	arg1	levels					1618:1623	the levels	1614:1623	the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins	1614:1687	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	10	2	theme	DHA-derived	1667:1677	arg1	oxylipins					1679:1687	PUFA-, ALA- and DHA-derived oxylipins	1651:1687	almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins	1628:1687	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	1	3	theme	nutrition	223:231	arg1	guidelines					233:242	nutrition guidelines	223:242	nutrition guidelines	223:242	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	4	4	theme	egg	775:777	arg1	layer					779:783	white egg layer	769:783	white egg layer	769:783	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	4	theme	egg	775:777	arg1	weeks					789:793	20 weeks	786:793	20 weeks old	786:797	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	8	5	theme	Dietary	1418:1424	arg1	intake					1426:1431	Dietary intake	1418:1431	Dietary intake of ALA	1418:1438	Dietary intake of ALA contributed mainly to ALA-derived oxylipins.
37187351	3	6	theme	health	635:640	arg1	benefits					642:649	the potential nutritional and health benefits	605:649	the potential nutritional and health benefits to laying hens	605:664	OBJECTIVES The study was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA.
37187351	12	7	from	LPS	2124:2126	arg1	hens					2138:2141	laying hens	2131:2141	laying hens	2131:2141	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	5	8	dep	Escherichia	1075:1085	arg1	-derived					1101:1108	-derived	1101:1108	-derived	1101:1108	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	5	8	dep	Escherichia	1075:1085	arg1	coli					1087:1090	coli	1087:1090	coli	1087:1090	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	5	8	dep	Escherichia	1075:1085	arg1	coli					1096:1099	E. coli	1093:1099	E. coli	1093:1099	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	4	9	theme	omega-3	922:928	arg1	PUFA					930:933	total dietary omega-3 PUFA	908:933	total dietary omega-3 PUFA	908:933	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	3	10	theme	nutritional	619:629	arg1	benefits					642:649	the potential nutritional and health benefits	605:649	the potential nutritional and health benefits to laying hens	605:664	OBJECTIVES The study was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA.
37187351	0	11	theme	Laying	180:185	arg1	Hens					187:190	Laying Hens	180:190	Laying Hens	180:190	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	5	12	theme	collection	1162:1171	arg1	hours					1175:1179	terminal sample collection 4 hours post-challenge	1146:1194	terminal sample collection 4 hours post-challenge	1146:1194	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	11	13	theme	receptor	1885:1892	arg1	TLR-4					1894:1898	receptor TLR-4	1885:1898	receptor TLR-4 (P < 0.001)	1885:1910	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	11	13	theme	receptor	1885:1892	arg1	P					1901:1901	P < 0.001	1901:1909	P < 0.001	1901:1909	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	9	14	link	DHA-derived	1529:1539	arg1	oxylipins					1541:1549	DHA-derived oxylipins	1529:1549	DHA-derived oxylipins	1529:1549	Meanwhile, eicosapentaenoic acid (EPA)- and DHA-derived oxylipins were primarily influenced by DHA dietary intake.
37187351	10	15	theme	COX-2	1740:1744	arg1	expression					1726:1735	hepatic mRNA expression	1713:1735	hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins	1713:1808	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	6	16	theme	subsequent	1259:1268	arg1	analyses					1270:1277	subsequent analyses	1259:1277	subsequent analyses	1259:1277	Egg yolk, plasma, liver and spleen samples were collected for subsequent analyses.
37187351	4	17	dep	METHODS	726:732	arg1	assigned					814:821	assigned	814:821	were randomly assigned to 1 of 8 treatment diets	800:847	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	18	theme	total	908:912	arg1	PUFA					930:933	total dietary omega-3 PUFA	908:933	total dietary omega-3 PUFA	908:933	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	3	19	theme	omega-3	689:695	arg1	PUFA					697:700	dietary omega-3 PUFA	681:700	dietary omega-3 PUFA	681:700	OBJECTIVES The study was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA.
37187351	0	20	theme	Fatty	117:121	arg1	Composition					128:138	Fatty Acid Composition	117:138	Fatty Acid Composition	117:138	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	7	21	dep	yolk	1368:1371	arg1	concentrations					1402:1415	fatty acid concentrations	1391:1415	fatty acid concentrations	1391:1415	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	6	22	theme	Egg	1197:1199	arg1	yolk					1201:1204	Egg yolk	1197:1204	Egg yolk	1197:1204	Egg yolk, plasma, liver and spleen samples were collected for subsequent analyses.
37187351	7	23	theme	fatty	1391:1395	arg1	concentrations					1402:1415	fatty acid concentrations	1391:1415	fatty acid concentrations	1391:1415	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	12	24	theme	inflammatory	2073:2084	arg1	responses					2086:2094	inflammatory responses	2073:2094	inflammatory responses	2073:2094	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	10	25	theme	5-LOX	1750:1754	arg1	expression					1726:1735	hepatic mRNA expression	1713:1735	hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins	1713:1808	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	11	26	theme	pro-inflammatory	1849:1864	arg1	cytokine					1866:1873	pro-inflammatory cytokine IFN-γ	1849:1879	pro-inflammatory cytokine IFN-γ	1849:1879	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	2	27	theme	immune	474:479	arg1	responses					481:489	the birds' immune responses	463:489	the birds' immune responses	463:489	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	4	28	theme	DHA-enriched	980:991	arg1	biomass					999:1005	DHA-enriched algal biomass	980:1005	DHA-enriched algal biomass	980:1005	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	5	29	theme	Escherichia	1075:1085	arg1	LPS					1110:1112	Escherichia coli (E. coli)-derived LPS	1075:1112	Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection)	1075:1138	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	0	30	theme	Plasma	141:146	arg1	Oxylipin					148:155	Plasma Oxylipin	141:155	Plasma Oxylipin	141:155	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	4	31	theme	treatment	833:841	arg1	diets					843:847	1 of 8 treatment diets	826:847	1 of 8 treatment diets	826:847	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	0	32	from	Oil	76:78	arg1	Acids					49:53	Omega-3 Fatty Acids	35:53	Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	35:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	32	from	Oil	76:78	arg1	Levels					25:30	Increasing Levels	14:30	Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	14:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	8	33	theme	ALA-derived	1462:1472	arg1	oxylipins					1474:1482	ALA-derived oxylipins	1462:1482	ALA-derived oxylipins	1462:1482	Dietary intake of ALA contributed mainly to ALA-derived oxylipins.
37187351	0	34	theme	Acids	49:53	arg1	Levels					25:30	Increasing Levels	14:30	Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	14:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	12	35	from	hens	2138:2141	arg1	administration					2106:2119	the administration	2102:2119	the administration of LPS in laying hens	2102:2141	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	7	36	theme	egg	1364:1366	arg1	yolk					1368:1371	egg yolk	1364:1371	egg yolk	1364:1371	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	9	37	theme	DHA-derived	1529:1539	arg1	oxylipins					1541:1549	DHA-derived oxylipins	1529:1549	DHA-derived oxylipins	1529:1549	Meanwhile, eicosapentaenoic acid (EPA)- and DHA-derived oxylipins were primarily influenced by DHA dietary intake.
37187351	4	38	theme	ALA-rich	955:962	arg1	oil					973:975	ALA-rich flaxseed oil	955:975	ALA-rich flaxseed oil	955:975	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	10	39	theme	hepatic	1713:1719	arg1	expression					1726:1735	hepatic mRNA expression	1713:1735	hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins	1713:1808	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	12	40	from	impacts	2008:2014	arg1	deposition					2030:2039	fatty acid deposition	2019:2039	fatty acid deposition	2019:2039	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	40	from	impacts	2008:2014	arg1	oxylipins					2059:2067	their derived oxylipins	2045:2067	their derived oxylipins	2045:2067	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	11	41	theme	cytokine	1866:1873	arg1	expression					1835:1844	mRNA expression	1830:1844	mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen	1830:1924	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	12	42	theme	fatty	2019:2023	arg1	deposition					2030:2039	fatty acid deposition	2019:2039	fatty acid deposition	2019:2039	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	43	contain	had	1997:1999	arg2	responses					2086:2094	inflammatory responses	2073:2094	inflammatory responses	2073:2094	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	43	contain	had	1997:1999	arg1	intake					1975:1980	dietary intake	1967:1980	dietary intake of ALA and DHA	1967:1995	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	43	contain	had	1997:1999	arg2	impacts					2008:2014	unique impacts	2001:2014	unique impacts on fatty acid deposition and their derived oxylipins	2001:2067	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	44	theme	ALA	1985:1987	arg1	intake					1975:1980	dietary intake	1967:1980	dietary intake of ALA and DHA	1967:1995	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	0	45	theme	Preformed	83:91	arg1	Acid					109:112	Preformed Docosahexaenoic Acid	83:112	Preformed Docosahexaenoic Acid	83:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	8	46	link	ALA-derived	1462:1472	arg1	oxylipins					1474:1482	ALA-derived oxylipins	1462:1482	ALA-derived oxylipins	1462:1482	Dietary intake of ALA contributed mainly to ALA-derived oxylipins.
37187351	9	47	theme	DHA	1580:1582	arg1	intake					1592:1597	DHA dietary intake	1580:1597	DHA dietary intake	1580:1597	Meanwhile, eicosapentaenoic acid (EPA)- and DHA-derived oxylipins were primarily influenced by DHA dietary intake.
37187351	0	48	from	Levels	25:30	arg1	Acid					109:112	Preformed Docosahexaenoic Acid	83:112	Preformed Docosahexaenoic Acid	83:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	48	from	Levels	25:30	arg1	Oil					76:78	Flaxseed Oil	67:78	Flaxseed Oil	67:78	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	6	49	theme	yolk	1201:1204	arg1	samples					1232:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples were collected for subsequent analyses.
37187351	11	50	theme	<	1903:1903	arg1	TLR-4					1894:1898	receptor TLR-4	1885:1898	receptor TLR-4 (P < 0.001)	1885:1910	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	11	50	theme	<	1903:1903	arg1	P					1901:1901	P < 0.001	1901:1909	P < 0.001	1901:1909	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	2	51	theme	α-linolenic	369:379	arg1	ALA					387:389	ALA	387:389	ALA	387:389	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	2	51	theme	α-linolenic	369:379	arg1	acid					381:384	α-linolenic acid	369:384	α-linolenic acid (ALA)	369:390	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	7	52	theme	dietary	1299:1305	arg1	supplementation					1315:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	0	53	theme	Levels	25:30	arg1	Effect					4:9	The Effect	0:9	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.	0:191	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	1	54	theme	polyunsaturated	271:285	arg1	PUFA					300:303	PUFA	300:303	PUFA	300:303	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	1	54	theme	polyunsaturated	271:285	arg1	acids					293:297	omega-3 polyunsaturated fatty acids	263:297	omega-3 polyunsaturated fatty acids (PUFA)	263:304	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	7	55	from	responses	1351:1359	arg1	liver					1385:1389	liver	1385:1389	liver	1385:1389	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	7	55	from	responses	1351:1359	arg1	plasma					1374:1379	plasma	1374:1379	plasma	1374:1379	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	7	55	from	responses	1351:1359	arg1	yolk					1368:1371	egg yolk	1364:1371	egg yolk	1364:1371	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	7	56	theme	RESULTS	1280:1286	arg1	supplementation					1315:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	11	57	from	expression	1835:1844	arg1	spleen					1919:1924	the spleen	1915:1924	the spleen	1915:1924	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	2	58	theme	docosahexaenoic	399:413	arg1	DHA					421:423	DHA	421:423	DHA	421:423	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	2	58	theme	docosahexaenoic	399:413	arg1	acid					415:418	docosahexaenoic acid	399:418	docosahexaenoic acid (DHA)	399:424	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	10	59	link	DHA-derived	1667:1677	arg1	oxylipins					1679:1687	PUFA-, ALA- and DHA-derived oxylipins	1651:1687	almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins	1628:1687	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	4	60	dep	LSL-Classic	756:766	arg1	layer					779:783	white egg layer	769:783	white egg layer	769:783	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	60	dep	LSL-Classic	756:766	arg1	weeks					789:793	20 weeks	786:793	20 weeks old	786:797	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	1	61	theme	acids	293:297	arg1	feeding					252:258	the feeding	248:258	the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens	248:319	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	12	62	from	administration	2106:2119	arg1	hens					2138:2141	laying hens	2131:2141	laying hens	2131:2141	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	2	63	from	type	350:353	arg1	diet					433:436	the diet	429:436	the diet	429:436	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	10	64	dep	oxylipins	1679:1687	arg1	all					1635:1637	all	1635:1637	all	1635:1637	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	5	65	theme	sample	1155:1160	arg1	hours					1175:1179	terminal sample collection 4 hours post-challenge	1146:1194	terminal sample collection 4 hours post-challenge	1146:1194	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	5	66	theme	8-week	1021:1026	arg1	period					1036:1041	an 8-week feeding period	1018:1041	an 8-week feeding period	1018:1041	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	2	67	from	levels	359:364	arg1	diet					433:436	the diet	429:436	the diet	429:436	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	12	68	theme	LPS	2124:2126	arg1	administration					2106:2119	the administration	2102:2119	the administration of LPS in laying hens	2102:2141	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	2	69	dep	type	350:353	arg1	the					346:348	the	346:348	the	346:348	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	5	70	theme	i.v.	1124:1127	arg1	injection					1129:1137	i.v. injection	1124:1137	8 mg/kg; i.v. injection	1115:1137	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	8	71	theme	ALA	1436:1438	arg1	intake					1426:1431	Dietary intake	1418:1431	Dietary intake of ALA	1418:1438	Dietary intake of ALA contributed mainly to ALA-derived oxylipins.
37187351	0	72	from	Acid	109:112	arg1	Acids					49:53	Omega-3 Fatty Acids	35:53	Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	35:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	72	from	Acid	109:112	arg1	Levels					25:30	Increasing Levels	14:30	Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	14:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	3	73	theme	potential	609:617	arg1	benefits					642:649	the potential nutritional and health benefits	605:649	the potential nutritional and health benefits to laying hens	605:664	OBJECTIVES The study was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA.
37187351	0	74	theme	Immune	161:166	arg1	Response					168:175	Immune Response	161:175	Immune Response	161:175	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	6	75	theme	liver	1215:1219	arg1	samples					1232:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples were collected for subsequent analyses.
37187351	4	76	theme	LSL-Classic	756:766	arg1	total					736:740	A total	734:740	A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old)	734:798	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	6	77	theme	plasma	1207:1212	arg1	samples					1232:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples were collected for subsequent analyses.
37187351	1	78	theme	guidelines	233:242	arg1	lack					215:218	a lack	213:218	a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens	213:319	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	4	79	theme	white	769:773	arg1	layer					779:783	white egg layer	769:783	white egg layer	769:783	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	79	theme	white	769:773	arg1	weeks					789:793	20 weeks	786:793	20 weeks old	786:797	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	80	theme	PUFA	930:933	arg1	PUFA					930:933	total dietary omega-3 PUFA	908:933	total dietary omega-3 PUFA	908:933	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	80	theme	PUFA	930:933	arg1	%					903:903	0.8%	900:903	0.8% of total dietary omega-3 PUFA	900:933	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	80	theme	PUFA	930:933	arg1	%					882:882	0.2%	879:882	0.2%	879:882	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	80	theme	PUFA	930:933	arg1	%					888:888	0.4%	885:888	0.4%	885:888	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	80	theme	PUFA	930:933	arg1	%					894:894	0.6%	891:894	0.6%	891:894	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	0	81	theme	Hens	187:190	arg1	Response					168:175	Immune Response	161:175	Immune Response	161:175	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	81	theme	Hens	187:190	arg1	Composition					128:138	Fatty Acid Composition	117:138	Fatty Acid Composition	117:138	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	81	theme	Hens	187:190	arg1	Oxylipin					148:155	Plasma Oxylipin	141:155	Plasma Oxylipin	141:155	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	82	theme	Omega-3	35:41	arg1	Acids					49:53	Omega-3 Fatty Acids	35:53	Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	35:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	12	83	theme	acid	2025:2028	arg1	deposition					2030:2039	fatty acid deposition	2019:2039	fatty acid deposition	2019:2039	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	11	84	theme	TLR-4	1894:1898	arg1	expression					1835:1844	mRNA expression	1830:1844	mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen	1830:1924	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	5	85	theme	post-challenge	1181:1194	arg1	hours					1175:1179	terminal sample collection 4 hours post-challenge	1146:1194	terminal sample collection 4 hours post-challenge	1146:1194	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	12	86	dep	CONCLUSIONS	1927:1937	arg1	revealed					1953:1960	revealed	1953:1960	revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens	1953:2141	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	7	87	theme	acid	1397:1400	arg1	concentrations					1402:1415	fatty acid concentrations	1391:1415	fatty acid concentrations	1391:1415	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	4	88	theme	old	795:797	arg1	layer					779:783	white egg layer	769:783	white egg layer	769:783	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	88	theme	old	795:797	arg1	weeks					789:793	20 weeks	786:793	20 weeks old	786:797	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	4	89	theme	dietary	914:920	arg1	PUFA					930:933	total dietary omega-3 PUFA	908:933	total dietary omega-3 PUFA	908:933	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	3	90	theme	laying	654:659	arg1	hens					661:664	laying hens	654:664	laying hens	654:664	OBJECTIVES The study was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA.
37187351	0	91	theme	Acid	123:126	arg1	Composition					128:138	Fatty Acid Composition	117:138	Fatty Acid Composition	117:138	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	9	92	theme	eicosapentaenoic	1496:1511	arg1	EPA					1519:1521	EPA	1519:1521	EPA	1519:1521	Meanwhile, eicosapentaenoic acid (EPA)- and DHA-derived oxylipins were primarily influenced by DHA dietary intake.
37187351	9	92	theme	eicosapentaenoic	1496:1511	arg1	acid					1513:1516	eicosapentaenoic acid	1496:1516	eicosapentaenoic acid (EPA)-	1496:1523	Meanwhile, eicosapentaenoic acid (EPA)- and DHA-derived oxylipins were primarily influenced by DHA dietary intake.
37187351	4	93	theme	algal	993:997	arg1	biomass					999:1005	DHA-enriched algal biomass	980:1005	DHA-enriched algal biomass	980:1005	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	3	94	theme	dietary	681:687	arg1	PUFA					697:700	dietary omega-3 PUFA	681:700	dietary omega-3 PUFA	681:700	OBJECTIVES The study was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA.
37187351	11	95	theme	mRNA	1830:1833	arg1	expression					1835:1844	mRNA expression	1830:1844	mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen	1830:1924	LPS also increased mRNA expression of pro-inflammatory cytokine IFN-γ and receptor TLR-4 (P < 0.001) in the spleen.
37187351	12	96	theme	derived	2051:2057	arg1	oxylipins					2059:2067	their derived oxylipins	2045:2067	their derived oxylipins	2045:2067	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	4	97	theme	flaxseed	964:971	arg1	oil					973:975	ALA-rich flaxseed oil	955:975	ALA-rich flaxseed oil	955:975	METHODS A total of 80 Lohmann LSL-Classic (white egg layer, 20 weeks old) were randomly assigned to 1 of 8 treatment diets (10 hens/treatment), provided 0.2%, 0.4%, 0.6%, or 0.8% of total dietary omega-3 PUFA, provided as either ALA-rich flaxseed oil or DHA-enriched algal biomass.
37187351	5	98	dep	LPS	1110:1112	arg1	mg/kg					1117:1121	8 mg/kg	1115:1121	8 mg/kg; i.v. injection	1115:1137	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	10	99	theme	<	1759:1759	arg1	COX-2					1740:1744	COX-2	1740:1744	COX-2	1740:1744	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	10	99	theme	<	1759:1759	arg1	P					1757:1757	P < 0.001	1757:1765	P < 0.001	1757:1765	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	0	100	theme	Flaxseed	67:74	arg1	Oil					76:78	Flaxseed Oil	67:78	Flaxseed Oil	67:78	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	7	101	theme	predictable	1339:1349	arg1	responses					1351:1359	predictable responses	1339:1359	predictable responses in egg yolk, plasma and liver fatty acid concentrations	1339:1415	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	0	102	from	Effect	4:9	arg1	Response					168:175	Immune Response	161:175	Immune Response	161:175	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	102	from	Effect	4:9	arg1	Composition					128:138	Fatty Acid Composition	117:138	Fatty Acid Composition	117:138	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	0	102	from	Effect	4:9	arg1	Oxylipin					148:155	Plasma Oxylipin	141:155	Plasma Oxylipin	141:155	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	1	103	theme	laying	309:314	arg1	hens					316:319	laying hens	309:319	laying hens	309:319	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	12	104	theme	DHA	1993:1995	arg1	intake					1975:1980	dietary intake	1967:1980	dietary intake of ALA and DHA	1967:1995	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	105	theme	dietary	1967:1973	arg1	intake					1975:1980	dietary intake	1967:1980	dietary intake of ALA and DHA	1967:1995	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	0	106	theme	Docosahexaenoic	93:107	arg1	Acid					109:112	Preformed Docosahexaenoic Acid	83:112	Preformed Docosahexaenoic Acid	83:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	12	107	theme	unique	2001:2006	arg1	impacts					2008:2014	unique impacts	2001:2014	unique impacts on fatty acid deposition and their derived oxylipins	2001:2067	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	108	from	responses	2086:2094	arg1	deposition					2030:2039	fatty acid deposition	2019:2039	fatty acid deposition	2019:2039	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	12	108	from	responses	2086:2094	arg1	oxylipins					2059:2067	their derived oxylipins	2045:2067	their derived oxylipins	2045:2067	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	10	109	theme	mRNA	1721:1724	arg1	expression					1726:1735	hepatic mRNA expression	1713:1735	hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins	1713:1808	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	9	110	theme	dietary	1584:1590	arg1	intake					1592:1597	DHA dietary intake	1580:1597	DHA dietary intake	1580:1597	Meanwhile, eicosapentaenoic acid (EPA)- and DHA-derived oxylipins were primarily influenced by DHA dietary intake.
37187351	0	111	theme	Increasing	14:23	arg1	Levels					25:30	Increasing Levels	14:30	Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	14:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	2	112	theme	acid	381:384	arg1	levels					359:364	levels	359:364	levels	359:364	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	2	112	theme	acid	381:384	arg1	type					350:353	type	350:353	type	350:353	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	1	113	theme	omega-3	263:269	arg1	PUFA					300:303	PUFA	300:303	PUFA	300:303	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	1	113	theme	omega-3	263:269	arg1	acids					293:297	omega-3 polyunsaturated fatty acids	263:297	omega-3 polyunsaturated fatty acids (PUFA)	263:304	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	7	114	theme	omega-3	1307:1313	arg1	supplementation					1315:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	6	115	theme	spleen	1225:1230	arg1	samples					1232:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples	1197:1238	Egg yolk, plasma, liver and spleen samples were collected for subsequent analyses.
37187351	2	116	theme	acid	415:418	arg1	levels					359:364	levels	359:364	levels	359:364	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	2	116	theme	acid	415:418	arg1	type					350:353	type	350:353	type	350:353	Knowledge as to whether the type and levels of α-linolenic acid (ALA) and/or docosahexaenoic acid (DHA) in the diet can make a difference to the birds' immune responses when subjected to a lipopolysaccharide (LPS) challenge is limited.
37187351	0	117	theme	Fatty	43:47	arg1	Acids					49:53	Omega-3 Fatty Acids	35:53	Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid	35:112	The Effect of Increasing Levels of Omega-3 Fatty Acids from either Flaxseed Oil or Preformed Docosahexaenoic Acid on Fatty Acid Composition, Plasma Oxylipin and Immune Response of Laying Hens.
37187351	1	118	theme	fatty	287:291	arg1	PUFA					300:303	PUFA	300:303	PUFA	300:303	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	1	118	theme	fatty	287:291	arg1	acids					293:297	omega-3 polyunsaturated fatty acids	263:297	omega-3 polyunsaturated fatty acids (PUFA)	263:304	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	7	119	theme	Increasing	1288:1297	arg1	supplementation					1315:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation	1280:1329	RESULTS Increasing dietary omega-3 supplementation yielded predictable responses in egg yolk, plasma and liver fatty acid concentrations.
37187351	5	120	dep	mg/kg	1117:1121	arg1	injection					1129:1137	i.v. injection	1124:1137	8 mg/kg; i.v. injection	1115:1137	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	10	121	theme	ALA-	1658:1661	arg1	oxylipins					1679:1687	PUFA-, ALA- and DHA-derived oxylipins	1651:1687	almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins	1628:1687	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	5	122	theme	terminal	1146:1153	arg1	hours					1175:1179	terminal sample collection 4 hours post-challenge	1146:1194	terminal sample collection 4 hours post-challenge	1146:1194	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	10	123	theme	PUFA-	1651:1655	arg1	oxylipins					1679:1687	PUFA-, ALA- and DHA-derived oxylipins	1651:1687	almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins	1628:1687	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	1	124	dep	BACKGROUND	193:202	arg1	is					210:211	is	210:211	is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens	210:319	BACKGROUND There is a lack of nutrition guidelines for the feeding of omega-3 polyunsaturated fatty acids (PUFA) to laying hens.
37187351	5	125	theme	feeding	1028:1034	arg1	period					1036:1041	an 8-week feeding period	1018:1041	an 8-week feeding period	1018:1041	Following an 8-week feeding period, the birds were challenged with Escherichia coli (E. coli)-derived LPS (8 mg/kg; i.v. injection), with terminal sample collection 4 hours post-challenge.
37187351	12	126	link	derived	2051:2057	arg1	oxylipins					2059:2067	their derived oxylipins	2045:2067	their derived oxylipins	2045:2067	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	10	127	theme	oxylipins	1800:1808	arg1	biosynthesis					1784:1795	the biosynthesis	1780:1795	the biosynthesis of oxylipins	1780:1808	LPS increased the levels of almost all the omega-6 PUFA-, ALA- and DHA-derived oxylipins in plasma and decreased hepatic mRNA expression of COX-2 and 5-LOX (P < 0.001) involved in the biosynthesis of oxylipins.
37187351	12	128	theme	laying	2131:2136	arg1	hens					2138:2141	laying hens	2131:2141	laying hens	2131:2141	CONCLUSIONS These results revealed that dietary intake of ALA and DHA had unique impacts on fatty acid deposition and their derived oxylipins and inflammatory responses under the administration of LPS in laying hens.
37187351	3	129	dep	OBJECTIVES	558:567	arg1	designed					583:590	designed	583:590	was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA	579:723	OBJECTIVES The study was designed to determine the potential nutritional and health benefits to laying hens when receiving dietary omega-3 PUFA from either ALA or DHA.
36176019	7	0	theme	white	1289:1293	arg1	sugar					1295:1299	white sugar	1289:1299	white sugar	1289:1299	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	10	1	dep	CONCLUSIONS	1682:1692	arg1	contribute					1707:1716	contribute	1707:1716	contribute a small proportion of total energy and nutrient intakes in the Australian population	1707:1801	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	3	2	theme	daily	311:315	arg1	intakes					317:323	daily intakes	311:323	daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes	311:530	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	8	3	theme	%	1496:1496	arg1	median					1370:1375	a median	1368:1375	a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake	1368:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	3	theme	%	1496:1496	arg1	range					1392:1396	interquartile range	1378:1396	interquartile range	1378:1396	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	4	theme	%	1405:1405	arg1	median					1370:1375	a median	1368:1375	a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake	1368:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	4	theme	%	1405:1405	arg1	range					1392:1396	interquartile range	1378:1396	interquartile range	1378:1396	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	10	5	theme	total	1740:1744	arg1	energy					1746:1751	total energy	1740:1751	total energy	1740:1751	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	6	6	theme	median	1112:1117	arg1	range					1134:1138	median [interquartile range	1112:1138	median [interquartile range	1112:1138	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	3	7	theme	selected	506:513	arg1	intakes					524:530	selected nutrient intakes	506:530	selected nutrient intakes	506:530	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	5	8	dep	items	688:692	arg1	i.e.					673:676	i.e.	673:676	i.e.	673:676	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	9	9	theme	largest	1541:1547	arg1	contribution					1549:1560	the largest contribution	1537:1560	the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%)	1537:1679	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	8	10	theme	intake	1520:1525	arg1	0.1-6.6					1464:1470	0.1-6.6	1464:1470	0.1-6.6	1464:1470	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	energy					1426:1431	total energy	1420:1431	total energy	1420:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	%					1496:1496	0.2%	1493:1496	0.2% (0.01-1.2) of protein intake	1493:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	0.01-1.2					1499:1506	0.01-1.2	1499:1506	0.01-1.2	1499:1506	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	fat					1453:1455	fat	1453:1455	fat	1453:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	1.1-7.9					1408:1414	1.1-7.9	1408:1414	1.1-7.9	1408:1414	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	carbohydrate					1476:1487	carbohydrate	1476:1487	carbohydrate	1476:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	%					1437:1437	5.3%	1434:1437	5.3% (0.0-15.5) of fat	1434:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	0.0-15.5					1440:1447	0.0-15.5	1440:1447	0.0-15.5	1440:1447	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	%					1461:1461	2.3%	1458:1461	2.3% (0.1-6.6) of carbohydrate	1458:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	intake					1520:1525	protein intake	1512:1525	protein intake	1512:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	10	theme	intake	1520:1525	arg1	%					1405:1405	3.8%	1402:1405	3.8% (1.1-7.9) of total energy	1402:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	0.1-6.6					1464:1470	0.1-6.6	1464:1470	0.1-6.6	1464:1470	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	energy					1426:1431	total energy	1420:1431	total energy	1420:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	%					1496:1496	0.2%	1493:1496	0.2% (0.01-1.2) of protein intake	1493:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	0.01-1.2					1499:1506	0.01-1.2	1499:1506	0.01-1.2	1499:1506	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	fat					1453:1455	fat	1453:1455	fat	1453:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	1.1-7.9					1408:1414	1.1-7.9	1408:1414	1.1-7.9	1408:1414	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	carbohydrate					1476:1487	carbohydrate	1476:1487	carbohydrate	1476:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	%					1437:1437	5.3%	1434:1437	5.3% (0.0-15.5) of fat	1434:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	0.0-15.5					1440:1447	0.0-15.5	1440:1447	0.0-15.5	1440:1447	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	%					1461:1461	2.3%	1458:1461	2.3% (0.1-6.6) of carbohydrate	1458:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	intake					1520:1525	protein intake	1512:1525	protein intake	1512:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	11	theme	energy	1426:1431	arg1	%					1405:1405	3.8%	1402:1405	3.8% (1.1-7.9) of total energy	1402:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	6	12	dep	reported	1050:1057	arg1	RESULTS					1016:1022	RESULTS	1016:1022	RESULTS	1016:1022	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	5	13	theme	total	950:954	arg1	energy					962:967	total daily energy	950:967	total daily energy	950:967	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	9	14	dep	%	1599:1599	arg1	%					1618:1618	males median 3.4%	1602:1618	females median 3.6%; males median 3.4%	1581:1618	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	1	15	theme	dietary	214:220	arg1	intake					222:227	overall dietary intake	206:227	overall dietary intake	206:227	BACKGROUND Dietary assessment commonly focuses on particular foods/food groups as indicators of overall dietary intake.
36176019	4	16	theme	≥	622:622	arg1	years					626:630	≥ 2 years	622:630	≥ 2 years (53% female, 29% aged 31-50 years)	622:665	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	7	17	dep	butter	1335:1340	arg1	e.g.					1329:1332	e.g.	1329:1332	e.g.	1329:1332	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	5	18	dep	SCS	668:670	arg1	items					688:692	any food items	679:692	any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour	679:811	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	9	19	theme	females	1581:1587	arg1	%					1599:1599	females median 3.6%	1581:1599	females median 3.6%; males median 3.4%	1581:1618	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	4	20	theme	aged	649:652	arg1	%					647:647	29%	645:647	29% aged 31-50 years	645:664	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	1	21	theme	Dietary	121:127	arg1	assessment					129:138	BACKGROUND Dietary assessment	110:138	BACKGROUND Dietary assessment	110:138	BACKGROUND Dietary assessment commonly focuses on particular foods/food groups as indicators of overall dietary intake.
36176019	8	22	theme	fat	1453:1455	arg1	0.1-6.6					1464:1470	0.1-6.6	1464:1470	0.1-6.6	1464:1470	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	energy					1426:1431	total energy	1420:1431	total energy	1420:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	%					1496:1496	0.2%	1493:1496	0.2% (0.01-1.2) of protein intake	1493:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	0.01-1.2					1499:1506	0.01-1.2	1499:1506	0.01-1.2	1499:1506	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	fat					1453:1455	fat	1453:1455	fat	1453:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	1.1-7.9					1408:1414	1.1-7.9	1408:1414	1.1-7.9	1408:1414	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	carbohydrate					1476:1487	carbohydrate	1476:1487	carbohydrate	1476:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	%					1437:1437	5.3%	1434:1437	5.3% (0.0-15.5) of fat	1434:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	0.0-15.5					1440:1447	0.0-15.5	1440:1447	0.0-15.5	1440:1447	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	%					1461:1461	2.3%	1458:1461	2.3% (0.1-6.6) of carbohydrate	1458:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	intake					1520:1525	protein intake	1512:1525	protein intake	1512:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	22	theme	fat	1453:1455	arg1	%					1405:1405	3.8%	1402:1405	3.8% (1.1-7.9) of total energy	1402:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	10	23	theme	intakes	1766:1772	arg1	proportion					1726:1735	a small proportion	1718:1735	a small proportion of total energy and nutrient intakes in the Australian population	1718:1801	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	4	24	theme	female	637:642	arg1	%					635:635	53% female	633:642	53% female	633:642	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	9	25	theme	median	1608:1613	arg1	%					1618:1618	males median 3.4%	1602:1618	females median 3.6%; males median 3.4%	1581:1618	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	0	26	theme	nutrients	69:77	arg1	nutrients					69:77	nutrients	69:77	nutrients	69:77	Do sauces, condiments and seasonings contribute important amounts of nutrients to Australian dietary intakes?
36176019	0	26	theme	nutrients	69:77	arg1	amounts					58:64	important amounts	48:64	important amounts of nutrients	48:77	Do sauces, condiments and seasonings contribute important amounts of nutrients to Australian dietary intakes?
36176019	1	27	theme	foods/food	171:180	arg1	groups					182:187	particular foods/food groups	160:187	particular foods/food groups as indicators of overall dietary intake	160:227	BACKGROUND Dietary assessment commonly focuses on particular foods/food groups as indicators of overall dietary intake.
36176019	0	28	theme	Australian	82:91	arg1	intakes					101:107	Australian dietary intakes	82:107	Australian dietary intakes	82:107	Do sauces, condiments and seasonings contribute important amounts of nutrients to Australian dietary intakes?
36176019	9	29	dep	median	1668:1673	arg1	%					1659:1659	females median 3.0%	1641:1659	females median 3.0%	1641:1659	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	3	30	theme	Activity	433:440	arg1	NNPAS					450:454	NNPAS	450:454	NNPAS	450:454	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	3	30	theme	Activity	433:440	arg1	Survey					442:447	Physical Activity Survey	424:447	Physical Activity Survey (NNPAS)	424:455	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	4	31	dep	METHODS	533:539	arg1	data					555:558	NNPAS dietary data	541:558	METHODS NNPAS dietary data	533:558	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	8	32	theme	carbohydrate	1476:1487	arg1	0.1-6.6					1464:1470	0.1-6.6	1464:1470	0.1-6.6	1464:1470	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	energy					1426:1431	total energy	1420:1431	total energy	1420:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	%					1496:1496	0.2%	1493:1496	0.2% (0.01-1.2) of protein intake	1493:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	0.01-1.2					1499:1506	0.01-1.2	1499:1506	0.01-1.2	1499:1506	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	fat					1453:1455	fat	1453:1455	fat	1453:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	1.1-7.9					1408:1414	1.1-7.9	1408:1414	1.1-7.9	1408:1414	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	carbohydrate					1476:1487	carbohydrate	1476:1487	carbohydrate	1476:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	%					1437:1437	5.3%	1434:1437	5.3% (0.0-15.5) of fat	1434:1455	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	0.0-15.5					1440:1447	0.0-15.5	1440:1447	0.0-15.5	1440:1447	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	%					1461:1461	2.3%	1458:1461	2.3% (0.1-6.6) of carbohydrate	1458:1487	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	intake					1520:1525	protein intake	1512:1525	protein intake	1512:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	32	theme	carbohydrate	1476:1487	arg1	%					1405:1405	3.8%	1402:1405	3.8% (1.1-7.9) of total energy	1402:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	4	33	dep	%	635:635	arg1	%					647:647	29%	645:647	29% aged 31-50 years	645:664	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	8	34	theme	protein	1512:1518	arg1	intake					1520:1525	protein intake	1512:1525	protein intake	1512:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	9	35	theme	females	1641:1647	arg1	%					1659:1659	females median 3.0%	1641:1659	females median 3.0%	1641:1659	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	3	36	theme	National	401:408	arg1	Nutrition					410:418	Australian National Nutrition	390:418	Australian National Nutrition	390:418	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	4	37	theme	NNPAS	541:545	arg1	data					555:558	NNPAS dietary data	541:558	METHODS NNPAS dietary data	533:558	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	4	38	dep	aged	617:620	arg1	years					626:630	≥ 2 years	622:630	≥ 2 years (53% female, 29% aged 31-50 years)	622:665	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	3	39	theme	recent	383:388	arg1	Nutrition					410:418	Australian National Nutrition	390:418	Australian National Nutrition	390:418	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	10	40	theme	nutrient	1757:1764	arg1	intakes					1766:1772	nutrient intakes	1757:1772	nutrient intakes	1757:1772	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	3	41	theme	total	489:493	arg1	energy					495:500	total energy	489:500	total energy	489:500	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	8	42	theme	interquartile	1378:1390	arg1	median					1370:1375	a median	1368:1375	a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake	1368:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	42	theme	interquartile	1378:1390	arg1	range					1392:1396	interquartile range	1378:1396	interquartile range	1378:1396	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	7	43	dep	sugar	1295:1299	arg1	e.g.					1283:1286	e.g.	1283:1286	e.g.	1283:1286	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	10	44	theme	population	1864:1873	arg1	groups					1875:1880	population groups	1864:1880	population groups	1864:1880	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	3	45	theme	nutrient	515:522	arg1	intakes					524:530	selected nutrient intakes	506:530	selected nutrient intakes	506:530	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	9	46	dep	intakes	1632:1638	arg1	median					1668:1673	median	1668:1673	median	1668:1673	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	6	47	dep	range	1134:1138	arg1	[1-4					1144:1147	2 [1-4	1142:1147	2 [1-4	1142:1147	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	7	48	theme	sugar/sugar	1261:1271	arg1	products					1273:1280	sugar/sugar products	1261:1280	sugar/sugar products (e.g., white sugar) (35.0%)	1261:1308	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	7	48	theme	sugar/sugar	1261:1271	arg1	%					1307:1307	35.0%	1303:1307	35.0%	1303:1307	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	6	49	theme	[interquartile	1119:1132	arg1	range					1134:1138	median [interquartile range	1112:1138	median [interquartile range	1112:1138	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	10	50	from	proportion	1726:1735	arg1	population					1792:1801	the Australian population	1777:1801	the Australian population	1777:1801	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	5	51	theme	food	683:686	arg1	items					688:692	any food items	679:692	any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour	679:811	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	6	52	theme	recall	1104:1109	arg1	day					1093:1095	the day	1089:1095	the day of the recall	1089:1109	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	10	53	theme	small	1720:1724	arg1	proportion					1726:1735	a small proportion	1718:1735	a small proportion of total energy and nutrient intakes in the Australian population	1718:1801	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	8	54	theme	total	1420:1424	arg1	energy					1426:1431	total energy	1420:1431	total energy	1420:1431	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	1	55	theme	overall	206:212	arg1	intake					222:227	overall dietary intake	206:227	overall dietary intake	206:227	BACKGROUND Dietary assessment commonly focuses on particular foods/food groups as indicators of overall dietary intake.
36176019	3	56	theme	present	287:293	arg1	study					295:299	The present study	283:299	The present study	283:299	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	9	57	theme	vitamin	1570:1576	arg1	E					1578:1578	vitamin E	1570:1578	vitamin E (females median 3.6%; males median 3.4%)	1570:1619	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	5	58	theme	daily	956:960	arg1	energy					962:967	total daily energy	950:967	total daily energy	950:967	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	7	59	dep	meals	1196:1200	arg1	dinner					1221:1226	dinner	1221:1226	dinner	1221:1226	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	7	59	dep	meals	1196:1200	arg1	lunch					1214:1218	lunch	1214:1218	lunch	1214:1218	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	7	59	dep	meals	1196:1200	arg1	breakfast					1203:1211	breakfast	1203:1211	breakfast	1203:1211	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	10	60	theme	energy	1746:1751	arg1	proportion					1726:1735	a small proportion	1718:1735	a small proportion of total energy and nutrient intakes in the Australian population	1718:1801	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	4	61	theme	aged	617:620	arg1	individuals					605:615	12,153 individuals	598:615	12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years)	598:665	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	8	62	theme	%	1437:1437	arg1	median					1370:1375	a median	1368:1375	a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake	1368:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	62	theme	%	1437:1437	arg1	range					1392:1396	interquartile range	1378:1396	interquartile range	1378:1396	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	1	63	theme	BACKGROUND	110:119	arg1	assessment					129:138	BACKGROUND Dietary assessment	110:138	BACKGROUND Dietary assessment	110:138	BACKGROUND Dietary assessment commonly focuses on particular foods/food groups as indicators of overall dietary intake.
36176019	9	64	theme	median	1589:1594	arg1	%					1599:1599	females median 3.6%	1581:1599	females median 3.6%; males median 3.4%	1581:1618	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	9	65	dep	E	1578:1578	arg1	%					1599:1599	females median 3.6%	1581:1599	females median 3.6%; males median 3.4%	1581:1618	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	3	66	theme	condiments	336:345	arg1	intakes					317:323	daily intakes	311:323	daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes	311:530	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	7	67	theme	main	1191:1194	arg1	meals					1196:1200	main meals	1191:1200	main meals (breakfast, lunch, dinner) (73.9%)	1191:1235	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	7	67	theme	main	1191:1194	arg1	%					1234:1234	73.9%	1230:1234	73.9%	1230:1234	SCS were predominantly consumed within main meals (breakfast, lunch, dinner) (73.9%), and were predominantly sugar/sugar products (e.g., white sugar) (35.0%), or fats and oils (e.g., butter) (25.9%).
36176019	9	68	theme	males	1602:1606	arg1	%					1618:1618	males median 3.4%	1602:1618	females median 3.6%; males median 3.4%	1581:1618	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	1	69	theme	particular	160:169	arg1	groups					182:187	particular foods/food groups	160:187	particular foods/food groups as indicators of overall dietary intake	160:227	BACKGROUND Dietary assessment commonly focuses on particular foods/food groups as indicators of overall dietary intake.
36176019	3	70	theme	sauces	328:333	arg1	intakes					317:323	daily intakes	311:323	daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes	311:530	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	0	71	theme	dietary	93:99	arg1	intakes					101:107	Australian dietary intakes	82:107	Australian dietary intakes	82:107	Do sauces, condiments and seasonings contribute important amounts of nutrients to Australian dietary intakes?
36176019	8	72	theme	%	1461:1461	arg1	median					1370:1375	a median	1368:1375	a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake	1368:1525	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	8	72	theme	%	1461:1461	arg1	range					1392:1396	interquartile range	1378:1396	interquartile range	1378:1396	SCS contributed a median (interquartile range) of 3.8% (1.1-7.9) of total energy, 5.3% (0.0-15.5) of fat, 2.3% (0.1-6.6) of carbohydrate and 0.2% (0.01-1.2) of protein intake.
36176019	10	73	theme	Australian	1781:1790	arg1	population					1792:1801	the Australian population	1777:1801	the Australian population	1777:1801	CONCLUSIONS Although SCS contribute a small proportion of total energy and nutrient intakes in the Australian population, the contribution is more substantial for some nutrients and population groups.
36176019	3	74	theme	seasonings	351:360	arg1	intakes					317:323	daily intakes	311:323	daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes	311:530	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	9	75	theme	sodium	1625:1630	arg1	intakes					1632:1638	sodium intakes	1625:1638	sodium intakes (females median 3.0%; males median 2.9%)	1625:1679	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	5	76	theme	micronutrient	993:1005	arg1	intakes					1007:1013	micronutrient intakes	993:1013	micronutrient intakes	993:1013	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	3	77	theme	Physical	424:431	arg1	NNPAS					450:454	NNPAS	450:454	NNPAS	450:454	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	3	77	theme	Physical	424:431	arg1	Survey					442:447	Physical Activity Survey	424:447	Physical Activity Survey (NNPAS)	424:455	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	5	78	theme	dietary	847:853	arg1	data					855:858	the dietary data	843:858	the dietary data	843:858	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	4	79	theme	dietary	547:553	arg1	data					555:558	NNPAS dietary data	541:558	METHODS NNPAS dietary data	533:558	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	9	80	theme	median	1649:1654	arg1	%					1659:1659	females median 3.0%	1641:1659	females median 3.0%	1641:1659	SCS made the largest contribution towards vitamin E (females median 3.6%; males median 3.4%) and sodium intakes (females median 3.0%; males median 2.9%).
36176019	1	81	theme	intake	222:227	arg1	indicators					192:201	indicators	192:201	indicators of overall dietary intake	192:227	BACKGROUND Dietary assessment commonly focuses on particular foods/food groups as indicators of overall dietary intake.
36176019	0	82	theme	important	48:56	arg1	nutrients					69:77	nutrients	69:77	nutrients	69:77	Do sauces, condiments and seasonings contribute important amounts of nutrients to Australian dietary intakes?
36176019	0	82	theme	important	48:56	arg1	amounts					58:64	important amounts	48:64	important amounts of nutrients	48:77	Do sauces, condiments and seasonings contribute important amounts of nutrients to Australian dietary intakes?
36176019	5	83	theme	primary	909:915	arg1	composition					917:927	primary composition	909:927	primary composition	909:927	SCS (i.e., any food items not normally consumed as a food itself, consumed as an addition to a dish after cooking/preparation to enhance flavour) were identified/coded within the dietary data and reported in terms of how they were consumed, primary composition, and contribution to total daily energy and selected macro- and micronutrient intakes.
36176019	6	84	theme	Most	1024:1027	arg1	participants					1029:1040	Most participants	1024:1040	Most participants (85.1%)	1024:1048	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	6	84	theme	Most	1024:1027	arg1	%					1047:1047	85.1%	1043:1047	85.1%	1043:1047	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	3	85	theme	Australian	390:399	arg1	Nutrition					410:418	Australian National Nutrition	390:418	Australian National Nutrition	390:418	The present study describes daily intakes of sauces, condiments and seasonings (SCS) using the most recent Australian National Nutrition and Physical Activity Survey (NNPAS), as well as the contribution to total energy and selected nutrient intakes.
36176019	4	86	theme	24-h	582:585	arg1	recall					587:592	one 24-h recall	578:592	one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years)	578:665	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36176019	6	87	dep	consuming	1059:1067	arg1	range					1134:1138	median [interquartile range	1112:1138	median [interquartile range	1112:1138	RESULTS Most participants (85.1%) reported consuming at least one SCS on the day of the recall (median [interquartile range], 2 [1-4]).
36176019	4	88	dep	years	626:630	arg1	%					635:635	53% female	633:642	53% female	633:642	METHODS NNPAS dietary data were collected by one 24-h recall for 12,153 individuals aged ≥ 2 years (53% female, 29% aged 31-50 years).
36192578	5	0	theme	Infarct	758:764	arg1	size					766:769	Infarct size	758:769	Infarct size	758:769	Infarct size was measured after 24 h of reperfusion, while signaling pathways were evaluated after 15 min of reperfusion.
36192578	7	1	theme	dysbiotic	1115:1123	arg1	microbiota					1125:1134	the dysbiotic microbiota	1111:1134	the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics	1111:1254	Our results demonstrate a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics.
36192578	3	2	theme	dysbiotic	494:502	arg1	diet					521:524	a dysbiotic or non-dysbiotic diet	492:524	a dysbiotic or non-dysbiotic diet	492:524	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	7	3	theme	dysbiotic	1219:1227	arg1	microbiota					1229:1238	the dysbiotic microbiota	1215:1238	the dysbiotic microbiota	1215:1238	Our results demonstrate a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics.
36192578	9	4	theme	dysbiotic	1481:1489	arg1	microbiota					1491:1500	dysbiotic microbiota	1481:1500	dysbiotic microbiota	1481:1500	These results indicate that dysbiotic microbiota promotes an increase in infarct size, an effect that probiotics can attenuate.
36192578	4	5	theme	anterior	683:690	arg1	artery					701:706	the anterior coronary artery	679:706	the anterior coronary artery of the transplanted rats	679:731	Then, the anterior coronary artery of the transplanted rats was occluded for 30 min.
36192578	6	6	theme	plasma	903:908	arg1	concentration					910:922	plasma concentration	903:922	plasma concentration of LPS (lipopolysaccharides)	903:951	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	2	7	theme	intestinal	285:294	arg1	microbiota					296:305	the intestinal microbiota	281:305	the intestinal microbiota modulated by a diet creating dysbiosis	281:344	This study aims to demonstrate that the intestinal microbiota modulated by a diet creating dysbiosis increased the size of the myocardial infarction and that probiotics could attenuate this effect.
36192578	9	8	from	increase	1514:1521	arg1	size					1534:1537	infarct size	1526:1537	infarct size	1526:1537	These results indicate that dysbiotic microbiota promotes an increase in infarct size, an effect that probiotics can attenuate.
36192578	4	9	theme	coronary	692:699	arg1	artery					701:706	the anterior coronary artery	679:706	the anterior coronary artery of the transplanted rats	679:731	Then, the anterior coronary artery of the transplanted rats was occluded for 30 min.
36192578	8	10	theme	infarct	1274:1280	arg1	size					1282:1285	infarct size	1274:1285	infarct size	1274:1285	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	2	11	theme	infarction	383:392	arg1	size					360:363	the size	356:363	the size of the myocardial infarction	356:392	This study aims to demonstrate that the intestinal microbiota modulated by a diet creating dysbiosis increased the size of the myocardial infarction and that probiotics could attenuate this effect.
36192578	0	12	from	extent	40:45	arg1	rats					81:84	rats	81:84	rats	81:84	Dysbiotic microbiota contributes to the extent of acute myocardial infarction in rats.
36192578	8	13	from	phosphorylation	1350:1364	arg1	barrier					1425:1431	intestinal barrier resistance and Akt	1414:1450	barrier	1425:1431	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	8	13	from	phosphorylation	1350:1364	arg1	Akt					1448:1450	Akt	1448:1450	Akt	1448:1450	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	5	14	theme	reperfusion	798:808	arg1	24 h					790:793	24 h	790:793	24 h of reperfusion	790:808	Infarct size was measured after 24 h of reperfusion, while signaling pathways were evaluated after 15 min of reperfusion.
36192578	8	15	theme	firmicutes/bacteroidetes	1312:1335	arg1	ratio					1337:1341	a higher firmicutes/bacteroidetes ratio	1303:1341	a higher firmicutes/bacteroidetes ratio	1303:1341	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	3	16	from	rats	483:486	arg1	transplants					466:476	microbiota transplants	455:476	microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics	455:565	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	3	17	theme	probiotics	556:565	arg1	presence					533:540	presence	533:540	presence	533:540	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	3	17	theme	probiotics	556:565	arg1	absence					545:551	absence	545:551	absence	545:551	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	1	18	theme	specific	180:187	arg1	diabetes					235:242	diabetes	235:242	diabetes	235:242	Increasing evidence suggests that the intestinal microbiota composition could play a role in specific pathologies such as hypertension, obesity and diabetes.
36192578	1	18	theme	specific	180:187	arg1	obesity					223:229	obesity	223:229	obesity	223:229	Increasing evidence suggests that the intestinal microbiota composition could play a role in specific pathologies such as hypertension, obesity and diabetes.
36192578	1	18	theme	specific	180:187	arg1	pathologies					189:199	specific pathologies	180:199	specific pathologies such as hypertension, obesity and diabetes	180:242	Increasing evidence suggests that the intestinal microbiota composition could play a role in specific pathologies such as hypertension, obesity and diabetes.
36192578	1	18	theme	specific	180:187	arg1	hypertension					209:220	hypertension	209:220	hypertension	209:220	Increasing evidence suggests that the intestinal microbiota composition could play a role in specific pathologies such as hypertension, obesity and diabetes.
36192578	0	19	theme	Dysbiotic	0:8	arg1	microbiota					10:19	Dysbiotic microbiota	0:19	Dysbiotic microbiota	0:19	Dysbiotic microbiota contributes to the extent of acute myocardial infarction in rats.
36192578	7	20	from	size	1077:1080	arg1	animals					1085:1091	animals	1085:1091	animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics	1085:1254	Our results demonstrate a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics.
36192578	1	21	theme	Increasing	87:96	arg1	evidence					98:105	Increasing evidence	87:105	Increasing evidence	87:105	Increasing evidence suggests that the intestinal microbiota composition could play a role in specific pathologies such as hypertension, obesity and diabetes.
36192578	2	22	theme	myocardial	372:381	arg1	infarction					383:392	the myocardial infarction	368:392	the myocardial infarction	368:392	This study aims to demonstrate that the intestinal microbiota modulated by a diet creating dysbiosis increased the size of the myocardial infarction and that probiotics could attenuate this effect.
36192578	6	23	theme	NF-κB	968:972	arg1	resistance					891:900	Intestinal resistance	880:900	Intestinal resistance	880:900	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	23	theme	NF-κB	968:972	arg1	concentration					910:922	plasma concentration	903:922	plasma concentration of LPS (lipopolysaccharides)	903:951	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	23	theme	NF-κB	968:972	arg1	Akt					978:980	Akt	978:980	Akt	978:980	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	23	theme	NF-κB	968:972	arg1	composition					986:996	composition	986:996	composition	986:996	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	23	theme	NF-κB	968:972	arg1	activation					954:963	activation	954:963	activation of NF-κB	954:972	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	7	24	theme	larger	1062:1067	arg1	size					1077:1080	a larger infarct size	1060:1080	a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics	1060:1254	Our results demonstrate a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics.
36192578	8	25	from	ratio	1337:1341	arg1	barrier					1425:1431	intestinal barrier resistance and Akt	1414:1450	barrier	1425:1431	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	8	25	from	ratio	1337:1341	arg1	Akt					1448:1450	Akt	1448:1450	Akt	1448:1450	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	3	26	theme	microbiota	455:464	arg1	transplants					466:476	microbiota transplants	455:476	microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics	455:565	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	4	27	theme	rats	728:731	arg1	artery					701:706	the anterior coronary artery	679:706	the anterior coronary artery of the transplanted rats	679:731	Then, the anterior coronary artery of the transplanted rats was occluded for 30 min.
36192578	7	28	theme	infarct	1069:1075	arg1	size					1077:1080	a larger infarct size	1060:1080	a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics	1060:1254	Our results demonstrate a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics.
36192578	0	29	from	rats	81:84	arg1	extent					40:45	the extent	36:45	the extent of acute myocardial infarction in rats	36:84	Dysbiotic microbiota contributes to the extent of acute myocardial infarction in rats.
36192578	8	30	theme	NF-kB	1344:1348	arg1	phosphorylation					1350:1364	NF-kB phosphorylation and plasma LPS concentration	1344:1393	phosphorylation	1350:1364	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	9	31	theme	infarct	1526:1532	arg1	size					1534:1537	infarct size	1526:1537	infarct size	1526:1537	These results indicate that dysbiotic microbiota promotes an increase in infarct size, an effect that probiotics can attenuate.
36192578	8	32	theme	intestinal	1414:1423	arg1	barrier					1425:1431	intestinal barrier resistance and Akt	1414:1450	barrier	1425:1431	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	5	33	theme	reperfusion	867:877	arg1	15 min					857:862	15 min	857:862	15 min of reperfusion	857:877	Infarct size was measured after 24 h of reperfusion, while signaling pathways were evaluated after 15 min of reperfusion.
36192578	0	34	from	infarction	67:76	arg1	rats					81:84	rats	81:84	rats	81:84	Dysbiotic microbiota contributes to the extent of acute myocardial infarction in rats.
36192578	8	35	from	increase	1262:1269	arg1	size					1282:1285	infarct size	1274:1285	infarct size	1274:1285	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	8	36	theme	higher	1305:1310	arg1	firmicutes/bacteroidetes					1312:1335	higher firmicutes/bacteroidetes	1305:1335	a higher firmicutes/bacteroidetes ratio	1303:1341	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	0	37	theme	acute	50:54	arg1	infarction					67:76	acute myocardial infarction	50:76	acute myocardial infarction in rats	50:84	Dysbiotic microbiota contributes to the extent of acute myocardial infarction in rats.
36192578	6	38	theme	Intestinal	880:889	arg1	resistance					891:900	Intestinal resistance	880:900	Intestinal resistance	880:900	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	4	39	theme	transplanted	715:726	arg1	rats					728:731	the transplanted rats	711:731	the transplanted rats	711:731	Then, the anterior coronary artery of the transplanted rats was occluded for 30 min.
36192578	8	40	from	concentration	1381:1393	arg1	barrier					1425:1431	intestinal barrier resistance and Akt	1414:1450	barrier	1425:1431	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	8	40	from	concentration	1381:1393	arg1	Akt					1448:1450	Akt	1448:1450	Akt	1448:1450	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	3	41	from	transplants	466:476	arg1	presence					533:540	presence	533:540	presence	533:540	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	3	41	from	transplants	466:476	arg1	absence					545:551	absence	545:551	absence	545:551	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	3	42	dep	presence	533:540	arg1	the					529:531	the	529:531	the	529:531	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	8	43	theme	LPS	1377:1379	arg1	concentration					1381:1393	NF-kB phosphorylation and plasma LPS concentration	1344:1393	concentration	1381:1393	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	3	44	theme	antibiotic	653:662	arg1	therapy					664:670	antibiotic therapy	653:670	antibiotic therapy	653:670	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	8	45	theme	plasma	1370:1375	arg1	concentration					1381:1393	NF-kB phosphorylation and plasma LPS concentration	1344:1393	concentration	1381:1393	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	1	46	theme	microbiota	136:145	arg1	composition					147:157	the intestinal microbiota composition	121:157	the intestinal microbiota composition	121:157	Increasing evidence suggests that the intestinal microbiota composition could play a role in specific pathologies such as hypertension, obesity and diabetes.
36192578	1	47	theme	intestinal	125:134	arg1	composition					147:157	the intestinal microbiota composition	121:157	the intestinal microbiota composition	121:157	Increasing evidence suggests that the intestinal microbiota composition could play a role in specific pathologies such as hypertension, obesity and diabetes.
36192578	0	48	theme	infarction	67:76	arg1	extent					40:45	the extent	36:45	the extent of acute myocardial infarction in rats	36:84	Dysbiotic microbiota contributes to the extent of acute myocardial infarction in rats.
36192578	5	49	theme	signaling	817:825	arg1	pathways					827:834	signaling pathways	817:834	signaling pathways	817:834	Infarct size was measured after 24 h of reperfusion, while signaling pathways were evaluated after 15 min of reperfusion.
36192578	3	50	theme	non-dysbiotic	507:519	arg1	diet					521:524	a dysbiotic or non-dysbiotic diet	492:524	a dysbiotic or non-dysbiotic diet	492:524	To do this, microbiota transplants from rats fed a dysbiotic or non-dysbiotic diet in the presence or absence of probiotics were performed for 10 days on rats whose microbiota had been previously suppressed by antibiotic therapy.
36192578	6	51	theme	microbiota	1005:1014	arg1	resistance					891:900	Intestinal resistance	880:900	Intestinal resistance	880:900	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	51	theme	microbiota	1005:1014	arg1	concentration					910:922	plasma concentration	903:922	plasma concentration of LPS (lipopolysaccharides)	903:951	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	51	theme	microbiota	1005:1014	arg1	Akt					978:980	Akt	978:980	Akt	978:980	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	51	theme	microbiota	1005:1014	arg1	composition					986:996	composition	986:996	composition	986:996	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	51	theme	microbiota	1005:1014	arg1	activation					954:963	activation	954:963	activation of NF-κB	954:972	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	0	52	theme	myocardial	56:65	arg1	infarction					67:76	acute myocardial infarction	50:76	acute myocardial infarction in rats	50:84	Dysbiotic microbiota contributes to the extent of acute myocardial infarction in rats.
36192578	6	53	theme	LPS	927:929	arg1	resistance					891:900	Intestinal resistance	880:900	Intestinal resistance	880:900	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	53	theme	LPS	927:929	arg1	concentration					910:922	plasma concentration	903:922	plasma concentration of LPS (lipopolysaccharides)	903:951	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	53	theme	LPS	927:929	arg1	Akt					978:980	Akt	978:980	Akt	978:980	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	53	theme	LPS	927:929	arg1	composition					986:996	composition	986:996	composition	986:996	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	6	53	theme	LPS	927:929	arg1	activation					954:963	activation	954:963	activation of NF-κB	954:972	Intestinal resistance, plasma concentration of LPS (lipopolysaccharides), activation of NF-κB and Akt and composition of the microbiota were also measured.
36192578	7	54	theme	other	1171:1175	arg1	groups					1177:1182	the other groups	1167:1182	the other groups	1167:1182	Our results demonstrate a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics.
36192578	7	54	theme	other	1171:1175	arg1	those					1195:1199	those	1195:1199	those	1195:1199	Our results demonstrate a larger infarct size in animals transplanted with the dysbiotic microbiota without probiotics compared to the other groups, including those that received the dysbiotic microbiota with probiotics.
36192578	8	55	dep	barrier	1425:1431	arg1	resistance					1433:1442	resistance	1433:1442	resistance	1433:1442	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	8	56	from	decrease	1402:1409	arg1	barrier					1425:1431	intestinal barrier resistance and Akt	1414:1450	barrier	1425:1431	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36192578	8	56	from	decrease	1402:1409	arg1	Akt					1448:1450	Akt	1448:1450	Akt	1448:1450	This increase in infarct size correlates with a higher firmicutes/bacteroidetes ratio, NF-kB phosphorylation and plasma LPS concentration, and a decrease in intestinal barrier resistance and Akt.
36099695	7	0	theme	line	1197:1200	arg1	lysates					1159:1165	cell lysates	1154:1165	cell lysates of the colon cancer HT29 cell line	1154:1200	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	1	1	theme	target	287:292	arg1	residues					315:322	target identical amino acid residues	287:322	target identical amino acid residues	287:322	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	7	2	theme	HT29	1187:1190	arg1	line					1197:1200	the colon cancer HT29 cell line	1170:1200	the colon cancer HT29 cell line	1170:1200	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	1	3	theme	amino	304:308	arg1	residues					315:322	target identical amino acid residues	287:322	target identical amino acid residues	287:322	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	7	4	theme	colon	1174:1178	arg1	line					1197:1200	the colon cancer HT29 cell line	1170:1200	the colon cancer HT29 cell line	1170:1200	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	7	5	theme	phosphopeptides	1246:1260	arg1	number					1227:1232	a comparable number	1214:1232	a comparable number of enriched phosphopeptides as the coeluted method	1214:1283	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	9	6	theme	O-linked	1529:1536	arg1	glycopeptides					1538:1550	O-linked glycopeptides	1529:1550	O-linked glycopeptides	1529:1550	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	0	7	link	O-linked	53:60	arg1	glycopeptides					62:74	O-linked glycopeptides	53:74	O-linked glycopeptides	53:74	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	8	8	theme	O-linked	1320:1327	arg1	glycopeptides					1329:1341	identified O-linked glycopeptides	1309:1341	identified O-linked glycopeptides with our established method	1309:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	4	9	theme	simultaneous	688:699	arg1	enrichment					701:710	simultaneous enrichment	688:710	simultaneous enrichment	688:710	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	7	10	theme	cell	1154:1157	arg1	lysates					1159:1165	cell lysates	1154:1165	cell lysates of the colon cancer HT29 cell line	1154:1200	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	1	11	theme	posttranslational	234:250	arg1	phosphorylation					174:188	Protein phosphorylation	166:188	Protein phosphorylation	166:188	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	11	theme	posttranslational	234:250	arg1	PTMs					267:270	PTMs	267:270	PTMs	267:270	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	11	theme	posttranslational	234:250	arg1	modifications					252:264	two critical posttranslational modifications	221:264	two critical posttranslational modifications (PTMs)	221:271	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	11	theme	posttranslational	234:250	arg1	glycosylation					203:215	O-linked glycosylation	194:215	O-linked glycosylation	194:215	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	6	12	theme	O-linked	1049:1056	arg1	glycopeptide					1058:1069	the O-linked glycopeptide	1045:1069	the O-linked glycopeptide	1045:1069	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	12	theme	O-linked	1049:1056	arg1	low					1109:1111	low	1109:1111	low	1109:1111	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	12	theme	O-linked	1049:1056	arg1	%					1116:1116	∼3%	1114:1116	∼3%	1114:1116	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	4	13	link	O-linked	738:745	arg1	glycopeptides					747:759	O-linked glycopeptides	738:759	O-linked glycopeptides	738:759	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	8	14	theme	established	1352:1362	arg1	method					1364:1369	our established method	1348:1369	our established method	1348:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	7	15	theme	method	1144:1149	arg1	application					1124:1134	The application	1120:1134	The application of this method to cell lysates of the colon cancer HT29 cell line	1120:1200	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	6	16	link	O-linked	1049:1056	arg1	glycopeptide					1058:1069	the O-linked glycopeptide	1045:1069	the O-linked glycopeptide	1045:1069	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	16	link	O-linked	1049:1056	arg1	low					1109:1111	low	1109:1111	low	1109:1111	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	16	link	O-linked	1049:1056	arg1	%					1116:1116	∼3%	1114:1116	∼3%	1114:1116	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	9	17	gly	glycopeptides	1538:1550	arg2	glycopeptides					1538:1550	O-linked glycopeptides	1529:1550	O-linked glycopeptides	1529:1550	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	8	18	gly	glycopeptides	1329:1341	arg2	glycopeptides					1329:1341	identified O-linked glycopeptides	1309:1341	identified O-linked glycopeptides with our established method	1309:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	3	19	gly	glycopeptides	606:618	arg2	glycopeptides					606:618	O-linked glycopeptides	597:618	O-linked glycopeptides	597:618	Although several strategies have been established to enrich N-linked glycopeptides and phosphopeptides simultaneously, they are not appropriate for O-linked glycopeptides and phosphopeptides.
36099695	9	20	theme	simultaneous	1504:1515	arg1	analysis					1517:1524	the simultaneous analysis	1500:1524	the simultaneous analysis	1500:1524	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	1	21	theme	Protein	166:172	arg1	modifications					252:264	two critical posttranslational modifications	221:264	two critical posttranslational modifications (PTMs)	221:271	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	21	theme	Protein	166:172	arg1	phosphorylation					174:188	Protein phosphorylation	166:188	Protein phosphorylation	166:188	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	21	theme	Protein	166:172	arg1	glycosylation					203:215	O-linked glycosylation	194:215	O-linked glycosylation	194:215	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	5	22	theme	targeted	992:999	arg1	peptides					1001:1008	the targeted peptides	988:1008	the targeted peptides	988:1008	The established method exhibited high selectivity, repeatability, and recovery of the targeted peptides.
36099695	3	23	gly	glycopeptides	518:530	arg2	glycopeptides					518:530	N-linked glycopeptides	509:530	N-linked glycopeptides	509:530	Although several strategies have been established to enrich N-linked glycopeptides and phosphopeptides simultaneously, they are not appropriate for O-linked glycopeptides and phosphopeptides.
36099695	0	24	theme	titanium	113:120	arg1	chromatography					140:153	immobilized titanium (IV) ion affinity chromatography	101:153	immobilized titanium (IV) ion affinity chromatography	101:153	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	9	25	theme	crosstalk	1640:1648	arg1	function					1624:1631	the biological function	1609:1631	the biological function of PTM crosstalk	1609:1648	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	3	26	link	O-linked	597:604	arg1	glycopeptides					606:618	O-linked glycopeptides	597:618	O-linked glycopeptides	597:618	Although several strategies have been established to enrich N-linked glycopeptides and phosphopeptides simultaneously, they are not appropriate for O-linked glycopeptides and phosphopeptides.
36099695	4	27	theme	affinity	859:866	arg1	Ti4+-IMAC					884:892	Ti4+-IMAC	884:892	Ti4+-IMAC	884:892	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	27	theme	affinity	859:866	arg1	chromatography					868:881	immobilized titanium (IV) ion affinity chromatography	829:881	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	1	28	link	O-linked	194:201	arg1	phosphorylation					174:188	Protein phosphorylation	166:188	Protein phosphorylation	166:188	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	28	link	O-linked	194:201	arg1	modifications					252:264	two critical posttranslational modifications	221:264	two critical posttranslational modifications (PTMs)	221:271	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	28	link	O-linked	194:201	arg1	glycosylation					203:215	O-linked glycosylation	194:215	O-linked glycosylation	194:215	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	4	29	theme	immobilized	829:839	arg1	Ti4+-IMAC					884:892	Ti4+-IMAC	884:892	Ti4+-IMAC	884:892	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	29	theme	immobilized	829:839	arg1	chromatography					868:881	immobilized titanium (IV) ion affinity chromatography	829:881	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	3	30	theme	several	458:464	arg1	strategies					466:475	several strategies	458:475	several strategies	458:475	Although several strategies have been established to enrich N-linked glycopeptides and phosphopeptides simultaneously, they are not appropriate for O-linked glycopeptides and phosphopeptides.
36099695	0	31	theme	Simultaneous	0:11	arg1	materials					155:163	materials	155:163	materials	155:163	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	31	theme	Simultaneous	0:11	arg1	enrichment					13:22	Simultaneous enrichment	0:22	Simultaneous enrichment	0:22	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	1	32	theme	acid	310:313	arg1	residues					315:322	target identical amino acid residues	287:322	target identical amino acid residues	287:322	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	9	33	link	O-linked	1529:1536	arg1	glycopeptides					1538:1550	O-linked glycopeptides	1529:1550	O-linked glycopeptides	1529:1550	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	5	34	theme	established	910:920	arg1	method					922:927	The established method	906:927	The established method	906:927	The established method exhibited high selectivity, repeatability, and recovery of the targeted peptides.
36099695	8	35	link	O-linked	1320:1327	arg1	glycopeptides					1329:1341	identified O-linked glycopeptides	1309:1341	identified O-linked glycopeptides with our established method	1309:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	7	36	theme	comparable	1216:1225	arg1	number					1227:1232	a comparable number	1214:1232	a comparable number of enriched phosphopeptides as the coeluted method	1214:1283	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	4	37	dep	enrichment	701:710	arg1	the					684:686	the	684:686	the	684:686	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	2	38	theme	crosstalk	365:373	arg1	Analysis					349:356	Analysis	349:356	Analysis of the crosstalk between these PTMs	349:392	Analysis of the crosstalk between these PTMs is crucial for deciphering their biological functions.
36099695	0	39	theme	O-linked	53:60	arg1	glycopeptides					62:74	O-linked glycopeptides	53:74	O-linked glycopeptides	53:74	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	1	40	dep	modifications	252:264	arg1	both					282:285	both	282:285	both	282:285	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	9	41	theme	established	1460:1470	arg1	beneficial					1485:1494	beneficial	1485:1494	beneficial	1485:1494	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	9	41	theme	established	1460:1470	arg1	strategy					1472:1479	the established strategy	1456:1479	the established strategy	1456:1479	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	8	42	dep	9.7-fold	1375:1382	arg1	9.7-fold					1375:1382	9.7-fold	1375:1382	9.7-fold	1375:1382	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	8	42	dep	9.7-fold	1375:1382	arg1	number					1299:1304	the number	1295:1304	the number of identified O-linked glycopeptides with our established method	1295:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	8	42	dep	9.7-fold	1375:1382	arg1	higher					1384:1389	higher	1384:1389	higher	1384:1389	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	9	43	theme	biological	1613:1622	arg1	function					1624:1631	the biological function	1609:1631	the biological function of PTM crosstalk	1609:1648	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	0	44	gly	glycopeptides	62:74	arg2	glycopeptides					62:74	O-linked glycopeptides	53:74	O-linked glycopeptides	53:74	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	7	45	theme	cell	1192:1195	arg1	line					1197:1200	the colon cancer HT29 cell line	1170:1200	the colon cancer HT29 cell line	1170:1200	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	0	46	with	separation	39:48	arg1	chromatography					140:153	immobilized titanium (IV) ion affinity chromatography	101:153	immobilized titanium (IV) ion affinity chromatography	101:153	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	7	47	theme	cancer	1180:1185	arg1	line					1197:1200	the colon cancer HT29 cell line	1170:1200	the colon cancer HT29 cell line	1170:1200	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	3	48	theme	O-linked	597:604	arg1	glycopeptides					606:618	O-linked glycopeptides	597:618	O-linked glycopeptides	597:618	Although several strategies have been established to enrich N-linked glycopeptides and phosphopeptides simultaneously, they are not appropriate for O-linked glycopeptides and phosphopeptides.
36099695	1	49	theme	identical	294:302	arg1	residues					315:322	target identical amino acid residues	287:322	target identical amino acid residues	287:322	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	3	50	link	N-linked	509:516	arg1	glycopeptides					518:530	N-linked glycopeptides	509:530	N-linked glycopeptides	509:530	Although several strategies have been established to enrich N-linked glycopeptides and phosphopeptides simultaneously, they are not appropriate for O-linked glycopeptides and phosphopeptides.
36099695	4	51	theme	O-linked	738:745	arg1	glycopeptides					747:759	O-linked glycopeptides	738:759	O-linked glycopeptides	738:759	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	1	52	theme	O-linked	194:201	arg1	phosphorylation					174:188	Protein phosphorylation	166:188	Protein phosphorylation	166:188	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	52	theme	O-linked	194:201	arg1	modifications					252:264	two critical posttranslational modifications	221:264	two critical posttranslational modifications (PTMs)	221:271	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	52	theme	O-linked	194:201	arg1	glycosylation					203:215	O-linked glycosylation	194:215	O-linked glycosylation	194:215	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	4	53	theme	sequential	716:725	arg1	elution					727:733	sequential elution	716:733	sequential elution	716:733	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	8	54	with	glycopeptides	1329:1341	arg1	method					1364:1369	our established method	1348:1369	our established method	1348:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	9	55	theme	glycopeptides	1538:1550	arg1	analysis					1517:1524	the simultaneous analysis	1500:1524	the simultaneous analysis	1500:1524	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	6	56	theme	phosphopeptide	1075:1088	arg1	fractions					1090:1098	phosphopeptide fractions	1075:1098	phosphopeptide fractions	1075:1098	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	8	57	theme	identified	1309:1318	arg1	glycopeptides					1329:1341	identified O-linked glycopeptides	1309:1341	identified O-linked glycopeptides with our established method	1309:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	0	58	theme	affinity	131:138	arg1	chromatography					140:153	immobilized titanium (IV) ion affinity chromatography	101:153	immobilized titanium (IV) ion affinity chromatography	101:153	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	6	59	gly	glycopeptide	1058:1069	arg2	glycopeptide					1058:1069	the O-linked glycopeptide	1045:1069	the O-linked glycopeptide	1045:1069	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	59	gly	glycopeptide	1058:1069	arg2	%					1116:1116	∼3%	1114:1116	∼3%	1114:1116	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	59	gly	glycopeptide	1058:1069	arg2	low					1109:1111	low	1109:1111	low	1109:1111	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	9	60	theme	phosphopeptides	1556:1570	arg1	analysis					1517:1524	the simultaneous analysis	1500:1524	the simultaneous analysis	1500:1524	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	8	61	theme	glycopeptides	1329:1341	arg1	9.7-fold					1375:1382	9.7-fold	1375:1382	9.7-fold	1375:1382	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	8	61	theme	glycopeptides	1329:1341	arg1	number					1299:1304	the number	1295:1304	the number of identified O-linked glycopeptides with our established method	1295:1369	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	8	61	theme	glycopeptides	1329:1341	arg1	higher					1384:1389	higher	1384:1389	higher	1384:1389	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	5	62	theme	high	939:942	arg1	selectivity					944:954	high selectivity	939:954	high selectivity	939:954	The established method exhibited high selectivity, repeatability, and recovery of the targeted peptides.
36099695	4	63	gly	glycopeptides	747:759	arg2	glycopeptides					747:759	O-linked glycopeptides	738:759	O-linked glycopeptides	738:759	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	5	64	theme	peptides	1001:1008	arg1	repeatability					957:969	repeatability	957:969	repeatability	957:969	The established method exhibited high selectivity, repeatability, and recovery of the targeted peptides.
36099695	5	64	theme	peptides	1001:1008	arg1	recovery					976:983	recovery	976:983	recovery	976:983	The established method exhibited high selectivity, repeatability, and recovery of the targeted peptides.
36099695	5	64	theme	peptides	1001:1008	arg1	selectivity					944:954	high selectivity	939:954	high selectivity	939:954	The established method exhibited high selectivity, repeatability, and recovery of the targeted peptides.
36099695	0	65	theme	phosphopeptides	80:94	arg1	materials					155:163	materials	155:163	materials	155:163	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	65	theme	phosphopeptides	80:94	arg1	enrichment					13:22	Simultaneous enrichment	0:22	Simultaneous enrichment	0:22	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	65	theme	phosphopeptides	80:94	arg1	separation					39:48	sequential separation	28:48	sequential separation	28:48	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	1	66	theme	critical	225:232	arg1	phosphorylation					174:188	Protein phosphorylation	166:188	Protein phosphorylation	166:188	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	66	theme	critical	225:232	arg1	PTMs					267:270	PTMs	267:270	PTMs	267:270	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	66	theme	critical	225:232	arg1	modifications					252:264	two critical posttranslational modifications	221:264	two critical posttranslational modifications (PTMs)	221:271	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	1	66	theme	critical	225:232	arg1	glycosylation					203:215	O-linked glycosylation	194:215	O-linked glycosylation	194:215	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	0	67	dep	materials	155:163	arg1	materials					155:163	materials	155:163	materials	155:163	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	67	dep	materials	155:163	arg1	enrichment					13:22	Simultaneous enrichment	0:22	Simultaneous enrichment	0:22	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	67	dep	materials	155:163	arg1	separation					39:48	sequential separation	28:48	sequential separation	28:48	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	4	68	theme	glycopeptides	747:759	arg1	enrichment					701:710	simultaneous enrichment	688:710	simultaneous enrichment	688:710	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	68	theme	glycopeptides	747:759	arg1	elution					727:733	sequential elution	716:733	sequential elution	716:733	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	0	69	theme	immobilized	101:111	arg1	chromatography					140:153	immobilized titanium (IV) ion affinity chromatography	101:153	immobilized titanium (IV) ion affinity chromatography	101:153	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	9	70	theme	function	1624:1631	arg1	study					1600:1604	the study	1596:1604	the study of the biological function of PTM crosstalk	1596:1648	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	0	71	theme	ion	127:129	arg1	chromatography					140:153	immobilized titanium (IV) ion affinity chromatography	101:153	immobilized titanium (IV) ion affinity chromatography	101:153	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	6	72	dep	low	1109:1111	arg1	glycopeptide					1058:1069	the O-linked glycopeptide	1045:1069	the O-linked glycopeptide	1045:1069	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	72	dep	low	1109:1111	arg1	low					1109:1111	low	1109:1111	low	1109:1111	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	6	72	dep	low	1109:1111	arg1	%					1116:1116	∼3%	1114:1116	∼3%	1114:1116	Particularly, the overlap between the O-linked glycopeptide and phosphopeptide fractions was very low (∼3%).
36099695	4	73	theme	phosphopeptides	765:779	arg1	enrichment					701:710	simultaneous enrichment	688:710	simultaneous enrichment	688:710	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	73	theme	phosphopeptides	765:779	arg1	elution					727:733	sequential elution	716:733	sequential elution	716:733	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	7	74	theme	coeluted	1269:1276	arg1	method					1278:1283	the coeluted method	1265:1283	the coeluted method	1265:1283	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	9	75	theme	PTM	1636:1638	arg1	crosstalk					1640:1648	PTM crosstalk	1636:1648	PTM crosstalk	1636:1648	Our study demonstrated that the established strategy was beneficial for the simultaneous analysis of O-linked glycopeptides and phosphopeptides, which might facilitate the study of the biological function of PTM crosstalk.
36099695	8	76	theme	coelution	1410:1418	arg1	method					1420:1425	the coelution method	1406:1425	the coelution method	1406:1425	However, the number of identified O-linked glycopeptides with our established method was 9.7-fold higher than that with the coelution method.
36099695	4	77	theme	IV	851:852	arg1	Ti4+-IMAC					884:892	Ti4+-IMAC	884:892	Ti4+-IMAC	884:892	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	77	theme	IV	851:852	arg1	chromatography					868:881	immobilized titanium (IV) ion affinity chromatography	829:881	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	78	theme	ion	855:857	arg1	Ti4+-IMAC					884:892	Ti4+-IMAC	884:892	Ti4+-IMAC	884:892	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	78	theme	ion	855:857	arg1	chromatography					868:881	immobilized titanium (IV) ion affinity chromatography	829:881	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	7	79	theme	enriched	1237:1244	arg1	phosphopeptides					1246:1260	enriched phosphopeptides	1237:1260	enriched phosphopeptides as the coeluted method	1237:1283	The application of this method to cell lysates of the colon cancer HT29 cell line resulted in a comparable number of enriched phosphopeptides as the coeluted method.
36099695	4	80	theme	available	819:827	arg1	materials					895:903	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	0	81	theme	sequential	28:37	arg1	materials					155:163	materials	155:163	materials	155:163	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	81	theme	sequential	28:37	arg1	separation					39:48	sequential separation	28:48	sequential separation	28:48	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	4	82	theme	titanium	841:848	arg1	Ti4+-IMAC					884:892	Ti4+-IMAC	884:892	Ti4+-IMAC	884:892	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	4	82	theme	titanium	841:848	arg1	chromatography					868:881	immobilized titanium (IV) ion affinity chromatography	829:881	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36099695	3	83	theme	N-linked	509:516	arg1	glycopeptides					518:530	N-linked glycopeptides	509:530	N-linked glycopeptides	509:530	Although several strategies have been established to enrich N-linked glycopeptides and phosphopeptides simultaneously, they are not appropriate for O-linked glycopeptides and phosphopeptides.
36099695	2	84	theme	biological	427:436	arg1	functions					438:446	their biological functions	421:446	their biological functions	421:446	Analysis of the crosstalk between these PTMs is crucial for deciphering their biological functions.
36099695	0	85	theme	glycopeptides	62:74	arg1	materials					155:163	materials	155:163	materials	155:163	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	85	theme	glycopeptides	62:74	arg1	enrichment					13:22	Simultaneous enrichment	0:22	Simultaneous enrichment	0:22	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	0	85	theme	glycopeptides	62:74	arg1	separation					39:48	sequential separation	28:48	sequential separation	28:48	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	1	86	theme	crosstalk	337:345	arg1	"					346:346	"crosstalk"	336:346	"crosstalk"	336:346	Protein phosphorylation and O-linked glycosylation are two critical posttranslational modifications (PTMs) and they both target identical amino acid residues, generating "crosstalk".
36099695	0	87	with	enrichment	13:22	arg1	chromatography					140:153	immobilized titanium (IV) ion affinity chromatography	101:153	immobilized titanium (IV) ion affinity chromatography	101:153	Simultaneous enrichment and sequential separation of O-linked glycopeptides and phosphopeptides with immobilized titanium (IV) ion affinity chromatography materials.
36099695	4	88	theme	chromatography	868:881	arg1	materials					895:903	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials	806:903	In this study, we established a method for the simultaneous enrichment and sequential elution of O-linked glycopeptides and phosphopeptides into two fractions using commercially available immobilized titanium (IV) ion affinity chromatography (Ti4+-IMAC) materials.
36683429	4	0	dep	lower	863:867	arg1	potential					887:895	inherent glycemic potential	869:895	inherent glycemic potential (60.4%)	869:903	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	4	0	dep	lower	863:867	arg1	%					902:902	60.4%	898:902	60.4%	898:902	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	3	1	dep	infrared	599:606	arg1	IR					609:610	IR	609:610	IR	609:610	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	4	2	theme	enzymatic	948:956	arg1	activities					958:967	lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities	863:967	lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour	863:1005	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	0	3	theme	pearl	140:144	arg1	rotis					153:157	pearl millet rotis	140:157	pearl millet rotis	140:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	3	4	theme	processing	534:543	arg1	methods					545:551	different thermal processing methods	516:551	different thermal processing methods	516:551	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	8	5	theme	phenolics	1570:1578	arg1	bioaccessibility					1550:1565	the bioaccessibility	1546:1565	the bioaccessibility of phenolics	1546:1578	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	0	6	theme	phenolics	108:116	arg1	potential					73:81	inherent glycemic potential	55:81	inherent glycemic potential	55:81	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	0	6	theme	phenolics	108:116	arg1	bioaccessibility					88:103	bioaccessibility	88:103	bioaccessibility of phenolics and micronutrients in pearl millet rotis	88:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	0	6	theme	phenolics	108:116	arg1	components					43:52	the matrix components	32:52	the matrix components	32:52	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	6	7	from	agglomeration	1273:1285	arg1	flour					1330:1334	the hydrothermally treated PM flour	1300:1334	the hydrothermally treated PM flour	1300:1334	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour was observed by SEM.
36683429	3	8	theme	different	516:524	arg1	methods					545:551	different thermal processing methods	516:551	different thermal processing methods	516:551	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	3	9	theme	microelements	668:680	arg1	digestion					632:640	the digestion	628:640	the digestion of starch, phenolics, and microelements (Fe and Zn)	628:692	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	5	10	from	ratio	1035:1039	arg1	sample					1089:1094	the hydrothermally treated sample	1062:1094	the hydrothermally treated sample	1062:1094	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	1	11	from	macro	212:216	arg1	rich					196:199	rich	196:199	rich	196:199	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	0	12	from	bioaccessibility	88:103	arg1	rotis					153:157	pearl millet rotis	140:157	pearl millet rotis	140:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	3	13	dep	in	698:699	arg1	vitro					701:705	vitro	701:705	vitro	701:705	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	8	14	theme	PM	1607:1608	arg1	rotis					1610:1614	native PM rotis	1600:1614	native PM rotis	1600:1614	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	3	15	theme	digestion	707:715	arg1	model					717:721	an in vitro digestion model	695:721	an in vitro digestion model consisting of oral, gastric and intestinal digestion	695:774	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	7	16	theme	digestion	1439:1447	arg1	rate					1449:1452	the lower starch digestion rate	1422:1452	the lower starch digestion rate in the hydrothermally treated flour	1422:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	2	17	theme	wide	468:471	arg1	popularity					473:482	wide popularity	468:482	wide popularity	468:482	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	6	18	theme	tighter	1225:1231	arg1	microstructure					1250:1263	A tighter and more compact microstructure	1223:1263	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour	1223:1334	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour was observed by SEM.
36683429	3	19	theme	gastric	743:749	arg1	digestion					766:774	oral, gastric and intestinal digestion	737:774	oral, gastric and intestinal digestion	737:774	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	7	20	theme	lower	1426:1430	arg1	rate					1449:1452	the lower starch digestion rate	1422:1452	the lower starch digestion rate in the hydrothermally treated flour	1422:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	4	21	theme	PM	998:999	arg1	flour					1001:1005	PM flour	998:1005	PM flour	998:1005	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	3	22	theme	in	698:699	arg1	model					717:721	an in vitro digestion model	695:721	an in vitro digestion model consisting of oral, gastric and intestinal digestion	695:774	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	0	23	theme	glycemic	64:71	arg1	potential					73:81	inherent glycemic potential	55:81	inherent glycemic potential	55:81	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	3	24	theme	oral	737:740	arg1	digestion					766:774	oral, gastric and intestinal digestion	737:774	oral, gastric and intestinal digestion	737:774	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	0	25	from	Effect	0:5	arg1	potential					73:81	inherent glycemic potential	55:81	inherent glycemic potential	55:81	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	0	25	from	Effect	0:5	arg1	bioaccessibility					88:103	bioaccessibility	88:103	bioaccessibility of phenolics and micronutrients in pearl millet rotis	88:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	0	25	from	Effect	0:5	arg1	components					43:52	the matrix components	32:52	the matrix components	32:52	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	8	26	theme	Fe	1641:1642	arg1	bioaccessibility					1663:1678	the Fe (2%) and Zn (3.2%) bioaccessibility	1637:1678	the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis	1637:1698	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	7	27	theme	better	1398:1403	arg1	understanding					1405:1417	a better understanding	1396:1417	a better understanding of the lower starch digestion rate in the hydrothermally treated flour	1396:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	4	28	theme	PM	828:829	arg1	promising					840:848	promising	840:848	promising	840:848	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	4	28	theme	PM	828:829	arg1	roti					831:834	The hydrothermally treated PM roti	801:834	The hydrothermally treated PM roti	801:834	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	7	29	from	flour	1484:1488	arg1	understanding					1405:1417	a better understanding	1396:1417	a better understanding of the lower starch digestion rate in the hydrothermally treated flour	1396:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	3	30	theme	intestinal	755:764	arg1	digestion					766:774	oral, gastric and intestinal digestion	737:774	oral, gastric and intestinal digestion	737:774	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	2	31	from	constraints	433:443	arg1	consumption					452:462	its consumption	448:462	its consumption	448:462	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	31	from	constraints	433:443	arg1	popularity					473:482	wide popularity	468:482	wide popularity	468:482	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	32	theme	lower	345:349	arg1	bioaccessibility					351:366	the lower bioaccessibility	341:366	the lower bioaccessibility of nutrients and rancidity of flour during storage	341:417	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	32	theme	lower	345:349	arg1	constraints					433:443	the major constraints	423:443	the major constraints in its consumption and wide popularity	423:482	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	5	33	theme	ordered	1144:1150	arg1	degree					1152:1157	the structurally ordered degree	1127:1157	the structurally ordered degree	1127:1157	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	0	34	theme	thermal	10:16	arg1	treatments					18:27	thermal treatments	10:27	thermal treatments	10:27	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	7	35	theme	structural	1363:1372	arg1	changes					1374:1380	These structural changes	1357:1380	These structural changes	1357:1380	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	2	36	theme	major	427:431	arg1	bioaccessibility					351:366	the lower bioaccessibility	341:366	the lower bioaccessibility of nutrients and rancidity of flour during storage	341:417	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	36	theme	major	427:431	arg1	constraints					433:443	the major constraints	423:443	the major constraints in its consumption and wide popularity	423:482	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	3	37	theme	microwave	579:587	arg1	treatments					613:622	hydrothermal (HT), microwave (MW), and infrared (IR) treatments	560:622	hydrothermal (HT), microwave (MW), and infrared (IR) treatments	560:622	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	2	38	theme	micronutrient	305:317	arg1	composition					319:329	a unique phenolic and micronutrient composition	283:329	a unique phenolic and micronutrient composition	283:329	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	0	39	theme	matrix	36:41	arg1	components					43:52	the matrix components	32:52	the matrix components	32:52	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	2	40	contain	have	278:281	arg1	grains					271:276	Its grains	267:276	Its grains	267:276	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	40	contain	have	278:281	arg2	composition					319:329	a unique phenolic and micronutrient composition	283:329	a unique phenolic and micronutrient composition	283:329	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	3	41	theme	infrared	599:606	arg1	treatments					613:622	hydrothermal (HT), microwave (MW), and infrared (IR) treatments	560:622	hydrothermal (HT), microwave (MW), and infrared (IR) treatments	560:622	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	2	42	theme	phenolic	292:299	arg1	composition					319:329	a unique phenolic and micronutrient composition	283:329	a unique phenolic and micronutrient composition	283:329	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	5	43	theme	cm-1	1054:1057	arg1	ratio					1035:1039	an increased ratio	1022:1039	an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample	1022:1094	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	8	44	from	rotis	1694:1698	arg1	present					1680:1686	present	1680:1686	present	1680:1686	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	5	45	theme	other	1197:1201	arg1	treatments					1211:1220	other thermal treatments	1197:1220	other thermal treatments	1197:1220	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	6	46	theme	PM	1327:1328	arg1	flour					1330:1334	the hydrothermally treated PM flour	1300:1334	the hydrothermally treated PM flour	1300:1334	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour was observed by SEM.
36683429	1	47	theme	various	204:210	arg1	macro					212:216	various macro	204:216	various macro	204:216	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	4	48	theme	inherent	869:876	arg1	potential					887:895	inherent glycemic potential	869:895	inherent glycemic potential (60.4%)	869:903	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	4	48	theme	inherent	869:876	arg1	%					902:902	60.4%	898:902	60.4%	898:902	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	4	49	from	rancidity	985:993	arg1	flour					1001:1005	PM flour	998:1005	PM flour	998:1005	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	8	50	theme	Zn	1653:1654	arg1	bioaccessibility					1663:1678	the Fe (2%) and Zn (3.2%) bioaccessibility	1637:1678	the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis	1637:1698	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	5	51	theme	increased	1025:1033	arg1	ratio					1035:1039	an increased ratio	1022:1039	an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample	1022:1094	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	1	52	theme	Pearl	160:164	arg1	PM					174:175	PM	174:175	PM	174:175	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	1	52	theme	Pearl	160:164	arg1	millet					166:171	Pearl millet	160:171	Pearl millet (PM)	160:176	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	1	52	theme	Pearl	160:164	arg1	nutri-cereal					183:194	a nutri-cereal	181:194	a nutri-cereal rich in various macro and micronutrients required for a balanced diet	181:264	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	1	53	from	micronutrients	222:235	arg1	rich					196:199	rich	196:199	rich	196:199	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	8	54	theme	present	1680:1686	arg1	bioaccessibility					1663:1678	the Fe (2%) and Zn (3.2%) bioaccessibility	1637:1678	the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis	1637:1698	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	3	55	theme	thermal	526:532	arg1	methods					545:551	different thermal processing methods	516:551	different thermal processing methods	516:551	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	3	56	theme	phenolics	653:661	arg1	digestion					632:640	the digestion	628:640	the digestion of starch, phenolics, and microelements (Fe and Zn)	628:692	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	8	57	theme	PM	1691:1692	arg1	rotis					1694:1698	PM rotis	1691:1698	PM rotis	1691:1698	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	3	58	dep	hydrothermal	560:571	arg1	HT					574:575	HT	574:575	HT	574:575	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	7	59	theme	treated	1476:1482	arg1	flour					1484:1488	the hydrothermally treated flour	1457:1488	the hydrothermally treated flour	1457:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	0	60	from	potential	73:81	arg1	rotis					153:157	pearl millet rotis	140:157	pearl millet rotis	140:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	4	61	theme	less	943:946	arg1	activities					958:967	lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities	863:967	lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour	863:1005	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	3	62	theme	starch	645:650	arg1	digestion					632:640	the digestion	628:640	the digestion of starch, phenolics, and microelements (Fe and Zn)	628:692	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	8	63	attach	present	1680:1686	arg1	rotis					1694:1698	PM rotis	1691:1698	PM rotis	1691:1698	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	8	63	attach	present	1680:1686	arg2	bioaccessibility					1663:1678	the Fe (2%) and Zn (3.2%) bioaccessibility	1637:1678	the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis	1637:1698	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	0	64	theme	millet	146:151	arg1	rotis					153:157	pearl millet rotis	140:157	pearl millet rotis	140:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	7	65	theme	rate	1449:1452	arg1	understanding					1405:1417	a better understanding	1396:1417	a better understanding of the lower starch digestion rate in the hydrothermally treated flour	1396:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	1	66	from	rich	196:199	arg1	micronutrients					222:235	micronutrients	222:235	micronutrients required for a balanced diet	222:264	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	1	66	from	rich	196:199	arg1	macro					212:216	various macro	204:216	various macro	204:216	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	7	67	theme	starch	1432:1437	arg1	rate					1449:1452	the lower starch digestion rate	1422:1452	the lower starch digestion rate in the hydrothermally treated flour	1422:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	8	68	dep	significantly	1513:1525	arg1	<					1530:1530	P < 0.05	1528:1535	P < 0.05	1528:1535	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	0	69	theme	inherent	55:62	arg1	potential					73:81	inherent glycemic potential	55:81	inherent glycemic potential	55:81	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	3	70	theme	hydrothermal	560:571	arg1	treatments					613:622	hydrothermal (HT), microwave (MW), and infrared (IR) treatments	560:622	hydrothermal (HT), microwave (MW), and infrared (IR) treatments	560:622	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	8	71	theme	native	1600:1605	arg1	rotis					1610:1614	native PM rotis	1600:1614	native PM rotis	1600:1614	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	1	72	theme	rich	196:199	arg1	millet					166:171	Pearl millet	160:171	Pearl millet (PM)	160:176	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	1	72	theme	rich	196:199	arg1	nutri-cereal					183:194	a nutri-cereal	181:194	a nutri-cereal rich in various macro and micronutrients required for a balanced diet	181:264	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	5	73	theme	treated	1081:1087	arg1	sample					1089:1094	the hydrothermally treated sample	1062:1094	the hydrothermally treated sample	1062:1094	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	3	74	dep	starch	645:650	arg1	Zn					690:691	Zn	690:691	Zn	690:691	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	3	74	dep	starch	645:650	arg1	Fe					683:684	Fe	683:684	Fe	683:684	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	7	75	from	rate	1449:1452	arg1	flour					1484:1488	the hydrothermally treated flour	1457:1488	the hydrothermally treated flour	1457:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	0	76	from	components	43:52	arg1	rotis					153:157	pearl millet rotis	140:157	pearl millet rotis	140:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	4	77	theme	treated	820:826	arg1	promising					840:848	promising	840:848	promising	840:848	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	4	77	theme	treated	820:826	arg1	roti					831:834	The hydrothermally treated PM roti	801:834	The hydrothermally treated PM roti	801:834	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	5	78	theme	starch	1178:1183	arg1	enhancement					1112:1122	the enhancement	1108:1122	the enhancement of the structurally ordered degree	1108:1157	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	5	78	theme	starch	1178:1183	arg1	compactness					1163:1173	compactness	1163:1173	compactness of starch	1163:1183	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	6	79	theme	starch	1290:1295	arg1	agglomeration					1273:1285	an agglomeration	1270:1285	an agglomeration of starch in the hydrothermally treated PM flour	1270:1334	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour was observed by SEM.
36683429	0	80	theme	micronutrients	122:135	arg1	potential					73:81	inherent glycemic potential	55:81	inherent glycemic potential	55:81	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	0	80	theme	micronutrients	122:135	arg1	bioaccessibility					88:103	bioaccessibility	88:103	bioaccessibility of phenolics and micronutrients in pearl millet rotis	88:157	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	0	80	theme	micronutrients	122:135	arg1	components					43:52	the matrix components	32:52	the matrix components	32:52	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	4	81	theme	glycemic	878:885	arg1	potential					887:895	inherent glycemic potential	869:895	inherent glycemic potential (60.4%)	869:903	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	4	81	theme	glycemic	878:885	arg1	%					902:902	60.4%	898:902	60.4%	898:902	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	8	82	dep	reduced	1538:1544	arg1	%					1585:1585	10.6%	1581:1585	10.6%	1581:1585	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	0	83	theme	treatments	18:27	arg1	Effect					0:5	Effect	0:5	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.	0:158	Effect of thermal treatments on the matrix components, inherent glycemic potential, and bioaccessibility of phenolics and micronutrients in pearl millet rotis.
36683429	1	84	theme	balanced	252:259	arg1	diet					261:264	a balanced diet	250:264	a balanced diet	250:264	Pearl millet (PM) is a nutri-cereal rich in various macro and micronutrients required for a balanced diet.
36683429	6	85	with	microstructure	1250:1263	arg1	agglomeration					1273:1285	an agglomeration	1270:1285	an agglomeration of starch in the hydrothermally treated PM flour	1270:1334	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour was observed by SEM.
36683429	2	86	theme	flour	398:402	arg1	rancidity					385:393	rancidity	385:393	rancidity of flour during storage	385:417	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	86	theme	flour	398:402	arg1	nutrients					371:379	nutrients	371:379	nutrients	371:379	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	5	87	theme	degree	1152:1157	arg1	enhancement					1112:1122	the enhancement	1108:1122	the enhancement of the structurally ordered degree	1108:1157	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	5	87	theme	degree	1152:1157	arg1	compactness					1163:1173	compactness	1163:1173	compactness of starch	1163:1183	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	2	88	theme	rancidity	385:393	arg1	bioaccessibility					351:366	the lower bioaccessibility	341:366	the lower bioaccessibility of nutrients and rancidity of flour during storage	341:417	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	88	theme	rancidity	385:393	arg1	constraints					433:443	the major constraints	423:443	the major constraints in its consumption and wide popularity	423:482	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	3	89	dep	treatments	613:622	arg1	i.e.					554:557	i.e.	554:557	i.e.	554:557	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	7	90	from	understanding	1405:1417	arg1	flour					1484:1488	the hydrothermally treated flour	1457:1488	the hydrothermally treated flour	1457:1488	These structural changes could provide a better understanding of the lower starch digestion rate in the hydrothermally treated flour.
36683429	3	91	theme	PM	791:792	arg1	rotis					794:798	PM rotis	791:798	PM rotis	791:798	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	8	92	from	present	1680:1686	arg1	rotis					1694:1698	PM rotis	1691:1698	PM rotis	1691:1698	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	2	93	theme	nutrients	371:379	arg1	bioaccessibility					351:366	the lower bioaccessibility	341:366	the lower bioaccessibility of nutrients and rancidity of flour during storage	341:417	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	2	93	theme	nutrients	371:379	arg1	constraints					433:443	the major constraints	423:443	the major constraints in its consumption and wide popularity	423:482	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	6	94	theme	compact	1242:1248	arg1	microstructure					1250:1263	A tighter and more compact microstructure	1223:1263	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour	1223:1334	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour was observed by SEM.
36683429	4	95	theme	lower	863:867	arg1	activities					958:967	lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities	863:967	lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour	863:1005	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	8	96	theme	HT	1500:1501	arg1	treatment					1503:1511	HT treatment	1500:1511	HT treatment	1500:1511	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	4	97	theme	untreated	914:922	arg1	sample					924:929	the untreated sample	910:929	the untreated sample (72.4%)	910:937	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	4	97	theme	untreated	914:922	arg1	%					936:936	72.4%	932:936	72.4%	932:936	The hydrothermally treated PM roti was promising as it showed lower inherent glycemic potential (60.4%) than the untreated sample (72.4%) and less enzymatic activities associated with rancidity in PM flour.
36683429	2	98	theme	unique	285:290	arg1	composition					319:329	a unique phenolic and micronutrient composition	283:329	a unique phenolic and micronutrient composition	283:329	Its grains have a unique phenolic and micronutrient composition; however, the lower bioaccessibility of nutrients and rancidity of flour during storage are the major constraints in its consumption and wide popularity.
36683429	3	99	dep	microwave	579:587	arg1	MW					590:591	MW	590:591	MW	590:591	Here, to explore the effect of different thermal processing methods, i.e., hydrothermal (HT), microwave (MW), and infrared (IR) treatments, on the digestion of starch, phenolics, and microelements (Fe and Zn), an in vitro digestion model consisting of oral, gastric and intestinal digestion was applied to PM rotis.
36683429	8	100	theme	P	1528:1528	arg1	<					1530:1530	P < 0.05	1528:1535	P < 0.05	1528:1535	However, HT treatment significantly (P < 0.05) reduced the bioaccessibility of phenolics (10.6%) compared to native PM rotis and slightly reduced the Fe (2%) and Zn (3.2%) bioaccessibility present in PM rotis.
36683429	5	101	theme	thermal	1203:1209	arg1	treatments					1211:1220	other thermal treatments	1197:1220	other thermal treatments	1197:1220	FTIR revealed an increased ratio of 1047/1022 cm-1 in the hydrothermally treated sample, reflecting the enhancement of the structurally ordered degree and compactness of starch compared to other thermal treatments.
36683429	6	102	theme	treated	1319:1325	arg1	flour					1330:1334	the hydrothermally treated PM flour	1300:1334	the hydrothermally treated PM flour	1300:1334	A tighter and more compact microstructure with an agglomeration of starch in the hydrothermally treated PM flour was observed by SEM.
35779098	9	0	theme	metabolic	1288:1296	arg1	disorders					1298:1306	metabolic disorders	1288:1306	metabolic disorders induced by antibiotic intervention	1288:1341	Thus, PB can alleviate metabolic disorders induced by antibiotic intervention, indicating a potential dietary strategy for populations with antibiotic-associated diarrhea.
35779098	12	1	theme	flora	1710:1714	arg1	alteration					1685:1694	cefixime-induced alteration	1668:1694	cefixime-induced alteration of intestinal flora	1668:1714	• Prebiotic blends relieve cefixime-induced alteration of intestinal flora by regulating metabolites, such as fatty acids and bile acids.
35779098	12	2	theme	bile	1767:1770	arg1	acids					1772:1776	bile acids	1767:1776	bile acids	1767:1776	• Prebiotic blends relieve cefixime-induced alteration of intestinal flora by regulating metabolites, such as fatty acids and bile acids.
35779098	4	3	theme	gut	486:488	arg1	microbes					490:497	gut microbes	486:497	gut microbes	486:497	Here, microbiome-metabolomics was used to evaluate how PB affect gut microbes and metabolic functions in C57BL/6 J mice administered cefixime.
35779098	5	4	theme	favorable	573:581	arg1	effects					583:589	favorable effects	573:589	favorable effects of PB on obesity outcomes	573:615	We found favorable effects of PB on obesity outcomes.
35779098	5	5	from	effects	583:589	arg1	outcomes					608:615	obesity outcomes	600:615	obesity outcomes	600:615	We found favorable effects of PB on obesity outcomes.
35779098	2	6	used	utilized	269:276	arg2	substrates					237:246	substrates	237:246	substrates that are selectively utilized by host microorganisms	237:299	Prebiotics are substrates that are selectively utilized by host microorganisms, conferring health benefits.
35779098	2	6	used	utilized	269:276	arg2	Prebiotics					222:231	Prebiotics	222:231	Prebiotics	222:231	Prebiotics are substrates that are selectively utilized by host microorganisms, conferring health benefits.
35779098	10	7	theme	•	1449:1449	arg1	blends					1461:1466	• Prebiotic blends	1449:1466	• Prebiotic blends	1449:1466	KEY POINTS: • Prebiotic blends significantly increased the Parabacteroides goldsteinii colony.
35779098	12	8	theme	fatty	1751:1755	arg1	acids					1757:1761	fatty acids	1751:1761	fatty acids	1751:1761	• Prebiotic blends relieve cefixime-induced alteration of intestinal flora by regulating metabolites, such as fatty acids and bile acids.
35779098	11	9	theme	antibiotic	1616:1625	arg1	intervention					1627:1638	antibiotic intervention	1616:1638	antibiotic intervention	1616:1638	• Prebiotic blends selectivity reversed this increase of Akkermansia muciniphila by antibiotic intervention.
35779098	2	10	theme	host	281:284	arg1	microorganisms					286:299	host microorganisms	281:299	host microorganisms	281:299	Prebiotics are substrates that are selectively utilized by host microorganisms, conferring health benefits.
35779098	6	11	theme	CEF	993:995	arg1	group					998:1002	the cefixime administration (CEF) group	964:1002	the cefixime administration (CEF) group	964:1002	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	3	12	theme	prebiotic	350:358	arg1	PB					368:369	PB	368:369	PB	368:369	Prebiotics, such as prebiotic blends (PB), are commonly used worldwide in food processing.
35779098	3	12	theme	prebiotic	350:358	arg1	blends					360:365	prebiotic blends	350:365	prebiotic blends (PB)	350:370	Prebiotics, such as prebiotic blends (PB), are commonly used worldwide in food processing.
35779098	11	13	theme	Akkermansia	1589:1599	arg1	muciniphila					1601:1611	Akkermansia muciniphila	1589:1611	Akkermansia muciniphila by antibiotic intervention	1589:1638	• Prebiotic blends selectivity reversed this increase of Akkermansia muciniphila by antibiotic intervention.
35779098	6	14	theme	Alloprevotella	712:725	arg1	abundance					665:673	the abundance	661:673	the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella	661:752	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	2	15	theme	health	313:318	arg1	benefits					320:327	health benefits	313:327	health benefits	313:327	Prebiotics are substrates that are selectively utilized by host microorganisms, conferring health benefits.
35779098	9	16	theme	antibiotic	1319:1328	arg1	intervention					1330:1341	antibiotic intervention	1319:1341	antibiotic intervention	1319:1341	Thus, PB can alleviate metabolic disorders induced by antibiotic intervention, indicating a potential dietary strategy for populations with antibiotic-associated diarrhea.
35779098	5	17	theme	PB	594:595	arg1	effects					583:589	favorable effects	573:589	favorable effects of PB on obesity outcomes	573:615	We found favorable effects of PB on obesity outcomes.
35779098	3	18	theme	food	404:407	arg1	processing					409:418	food processing	404:418	food processing	404:418	Prebiotics, such as prebiotic blends (PB), are commonly used worldwide in food processing.
35779098	8	19	theme	fatty	1237:1241	arg1	acids					1243:1247	unsaturated fatty acids	1225:1247	unsaturated fatty acids	1225:1247	In addition, it regulated the levels of microbial metabolites such as unsaturated fatty acids and bile acids.
35779098	6	20	theme	sensu	897:901	arg1	stricto					903:909	Clostridium sensu stricto 1	885:911	Clostridium sensu stricto 1	885:911	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	5	21	theme	obesity	600:606	arg1	outcomes					608:615	obesity outcomes	600:615	obesity outcomes	600:615	We found favorable effects of PB on obesity outcomes.
35779098	10	22	theme	Prebiotic	1451:1459	arg1	blends					1461:1466	• Prebiotic blends	1449:1466	• Prebiotic blends	1449:1466	KEY POINTS: • Prebiotic blends significantly increased the Parabacteroides goldsteinii colony.
35779098	8	23	theme	microbial	1195:1203	arg1	metabolites					1205:1215	microbial metabolites	1195:1215	microbial metabolites such as unsaturated fatty acids and bile acids	1195:1262	In addition, it regulated the levels of microbial metabolites such as unsaturated fatty acids and bile acids.
35779098	8	23	theme	microbial	1195:1203	arg1	acids					1258:1262	bile acids	1253:1262	bile acids	1253:1262	In addition, it regulated the levels of microbial metabolites such as unsaturated fatty acids and bile acids.
35779098	8	23	theme	microbial	1195:1203	arg1	acids					1243:1247	unsaturated fatty acids	1225:1247	unsaturated fatty acids	1225:1247	In addition, it regulated the levels of microbial metabolites such as unsaturated fatty acids and bile acids.
35779098	6	24	theme	Clostridium	885:895	arg1	stricto					903:909	Clostridium sensu stricto 1	885:911	Clostridium sensu stricto 1	885:911	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	10	25	theme	goldsteinii	1512:1522	arg1	colony					1524:1529	the Parabacteroides goldsteinii colony	1492:1529	the Parabacteroides goldsteinii colony	1492:1529	KEY POINTS: • Prebiotic blends significantly increased the Parabacteroides goldsteinii colony.
35779098	6	26	theme	administration	977:990	arg1	group					998:1002	the cefixime administration (CEF) group	964:1002	the cefixime administration (CEF) group	964:1002	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	1	27	theme	gut	174:176	arg1	microbiota					178:187	the gut microbiota	170:187	the gut microbiota	170:187	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	12	28	theme	•	1641:1641	arg1	blends					1653:1658	• Prebiotic blends	1641:1658	• Prebiotic blends	1641:1658	• Prebiotic blends relieve cefixime-induced alteration of intestinal flora by regulating metabolites, such as fatty acids and bile acids.
35779098	4	29	theme	C57BL/6 J	526:534	arg1	mice					536:539	C57BL/6 J mice	526:539	C57BL/6 J mice administered cefixime	526:561	Here, microbiome-metabolomics was used to evaluate how PB affect gut microbes and metabolic functions in C57BL/6 J mice administered cefixime.
35779098	8	30	theme	bile	1253:1256	arg1	acids					1258:1262	bile acids	1253:1262	bile acids	1253:1262	In addition, it regulated the levels of microbial metabolites such as unsaturated fatty acids and bile acids.
35779098	6	31	theme	Bifidobacterium	678:692	arg1	abundance					665:673	the abundance	661:673	the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella	661:752	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	1	32	theme	microbiota	178:187	arg1	composition					155:165	the composition	151:165	the composition of the gut microbiota, host diet, and host physiology	151:219	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	0	33	theme	gut	32:34	arg1	microbiota					36:45	cefixime-induced gut microbiota	15:45	cefixime-induced gut microbiota	15:45	Restoration of cefixime-induced gut microbiota changes by a prebiotic blend in a mouse model.
35779098	6	34	theme	cefixime	968:975	arg1	group					998:1002	the cefixime administration (CEF) group	964:1002	the cefixime administration (CEF) group	964:1002	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	7	35	theme	goldsteinii	1066:1076	arg1	abundance					1037:1045	the abundance	1033:1045	the abundance of Parabacteroides goldsteinii	1033:1076	In particular, PB increased the abundance of Parabacteroides goldsteinii and suppressed that of Robinsoniella peoriensis and Akkermansia muciniphila.
35779098	11	36	theme	muciniphila	1601:1611	arg1	increase					1577:1584	this increase	1572:1584	this increase of Akkermansia muciniphila by antibiotic intervention	1572:1638	• Prebiotic blends selectivity reversed this increase of Akkermansia muciniphila by antibiotic intervention.
35779098	9	37	with	populations	1388:1398	arg1	diarrhea					1427:1434	antibiotic-associated diarrhea	1405:1434	antibiotic-associated diarrhea	1405:1434	Thus, PB can alleviate metabolic disorders induced by antibiotic intervention, indicating a potential dietary strategy for populations with antibiotic-associated diarrhea.
35779098	0	38	theme	cefixime-induced	15:30	arg1	microbiota					36:45	cefixime-induced gut microbiota	15:45	cefixime-induced gut microbiota	15:45	Restoration of cefixime-induced gut microbiota changes by a prebiotic blend in a mouse model.
35779098	9	39	theme	potential	1357:1365	arg1	strategy					1375:1382	a potential dietary strategy	1355:1382	a potential dietary strategy for populations with antibiotic-associated diarrhea	1355:1434	Thus, PB can alleviate metabolic disorders induced by antibiotic intervention, indicating a potential dietary strategy for populations with antibiotic-associated diarrhea.
35779098	10	40	theme	KEY	1437:1439	arg1	POINTS					1441:1446	KEY POINTS	1437:1446	KEY POINTS: • Prebiotic blends significantly increased the Parabacteroides goldsteinii colony.	1437:1530	KEY POINTS: • Prebiotic blends significantly increased the Parabacteroides goldsteinii colony.
35779098	1	41	theme	Recent	94:99	arg1	studies					101:107	Recent studies	94:107	Recent studies	94:107	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	1	42	theme	host	190:193	arg1	diet					195:198	host diet	190:198	host diet	190:198	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	8	43	theme	unsaturated	1225:1235	arg1	acids					1243:1247	unsaturated fatty acids	1225:1247	unsaturated fatty acids	1225:1247	In addition, it regulated the levels of microbial metabolites such as unsaturated fatty acids and bile acids.
35779098	9	44	theme	dietary	1367:1373	arg1	strategy					1375:1382	a potential dietary strategy	1355:1382	a potential dietary strategy for populations with antibiotic-associated diarrhea	1355:1434	Thus, PB can alleviate metabolic disorders induced by antibiotic intervention, indicating a potential dietary strategy for populations with antibiotic-associated diarrhea.
35779098	11	45	theme	Prebiotic	1534:1542	arg1	selectivity					1551:1561	• Prebiotic blends selectivity	1532:1561	• Prebiotic blends selectivity	1532:1561	• Prebiotic blends selectivity reversed this increase of Akkermansia muciniphila by antibiotic intervention.
35779098	1	46	theme	diet	195:198	arg1	composition					155:165	the composition	151:165	the composition of the gut microbiota, host diet, and host physiology	151:219	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	0	47	theme	microbiota	36:45	arg1	Restoration					0:10	Restoration	0:10	Restoration of cefixime-induced gut microbiota	0:45	Restoration of cefixime-induced gut microbiota changes by a prebiotic blend in a mouse model.
35779098	7	48	theme	Parabacteroides	1050:1064	arg1	goldsteinii					1066:1076	Parabacteroides goldsteinii	1050:1076	Parabacteroides goldsteinii	1050:1076	In particular, PB increased the abundance of Parabacteroides goldsteinii and suppressed that of Robinsoniella peoriensis and Akkermansia muciniphila.
35779098	0	49	from	blend	70:74	arg1	model					87:91	a mouse model	79:91	a mouse model	79:91	Restoration of cefixime-induced gut microbiota changes by a prebiotic blend in a mouse model.
35779098	12	50	theme	Prebiotic	1643:1651	arg1	blends					1653:1658	• Prebiotic blends	1641:1658	• Prebiotic blends	1641:1658	• Prebiotic blends relieve cefixime-induced alteration of intestinal flora by regulating metabolites, such as fatty acids and bile acids.
35779098	12	51	theme	cefixime-induced	1668:1683	arg1	alteration					1685:1694	cefixime-induced alteration	1668:1694	cefixime-induced alteration of intestinal flora	1668:1714	• Prebiotic blends relieve cefixime-induced alteration of intestinal flora by regulating metabolites, such as fatty acids and bile acids.
35779098	4	52	used	used	455:458	arg2	microbiome-metabolomics					427:449	microbiome-metabolomics	427:449	microbiome-metabolomics	427:449	Here, microbiome-metabolomics was used to evaluate how PB affect gut microbes and metabolic functions in C57BL/6 J mice administered cefixime.
35779098	11	53	theme	blends	1544:1549	arg1	selectivity					1551:1561	• Prebiotic blends selectivity	1532:1561	• Prebiotic blends selectivity	1532:1561	• Prebiotic blends selectivity reversed this increase of Akkermansia muciniphila by antibiotic intervention.
35779098	4	54	theme	metabolic	503:511	arg1	functions					513:521	metabolic functions	503:521	metabolic functions	503:521	Here, microbiome-metabolomics was used to evaluate how PB affect gut microbes and metabolic functions in C57BL/6 J mice administered cefixime.
35779098	3	55	used	used	386:389	arg2	blends					360:365	prebiotic blends	350:365	prebiotic blends (PB)	350:370	Prebiotics, such as prebiotic blends (PB), are commonly used worldwide in food processing.
35779098	3	55	used	used	386:389	arg2	Prebiotics					330:339	Prebiotics	330:339	Prebiotics	330:339	Prebiotics, such as prebiotic blends (PB), are commonly used worldwide in food processing.
35779098	6	56	theme	Dubosiella	743:752	arg1	abundance					665:673	the abundance	661:673	the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella	661:752	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	10	57	dep	POINTS	1441:1446	arg1	increased					1482:1490	increased	1482:1490	increased the Parabacteroides goldsteinii colony	1482:1529	KEY POINTS: • Prebiotic blends significantly increased the Parabacteroides goldsteinii colony.
35779098	9	58	theme	antibiotic-associated	1405:1425	arg1	diarrhea					1427:1434	antibiotic-associated diarrhea	1405:1434	antibiotic-associated diarrhea	1405:1434	Thus, PB can alleviate metabolic disorders induced by antibiotic intervention, indicating a potential dietary strategy for populations with antibiotic-associated diarrhea.
35779098	12	59	theme	intestinal	1699:1708	arg1	flora					1710:1714	intestinal flora	1699:1714	intestinal flora	1699:1714	• Prebiotic blends relieve cefixime-induced alteration of intestinal flora by regulating metabolites, such as fatty acids and bile acids.
35779098	1	60	theme	compelling	123:132	arg1	evidence					134:141	compelling evidence	123:141	compelling evidence linking the composition of the gut microbiota, host diet, and host physiology	123:219	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	11	61	theme	•	1532:1532	arg1	selectivity					1551:1561	• Prebiotic blends selectivity	1532:1561	• Prebiotic blends selectivity	1532:1561	• Prebiotic blends selectivity reversed this increase of Akkermansia muciniphila by antibiotic intervention.
35779098	1	62	theme	host	205:208	arg1	physiology					210:219	host physiology	205:219	host physiology	205:219	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	10	63	theme	Parabacteroides	1496:1510	arg1	colony					1524:1529	the Parabacteroides goldsteinii colony	1492:1529	the Parabacteroides goldsteinii colony	1492:1529	KEY POINTS: • Prebiotic blends significantly increased the Parabacteroides goldsteinii colony.
35779098	6	64	theme	PB	618:619	arg1	supplementation					621:635	PB supplementation	618:635	PB supplementation	618:635	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	1	65	theme	physiology	210:219	arg1	composition					155:165	the composition	151:165	the composition of the gut microbiota, host diet, and host physiology	151:219	Recent studies have provided compelling evidence linking the composition of the gut microbiota, host diet, and host physiology.
35779098	0	66	theme	prebiotic	60:68	arg1	blend					70:74	a prebiotic blend	58:74	a prebiotic blend in a mouse model	58:91	Restoration of cefixime-induced gut microbiota changes by a prebiotic blend in a mouse model.
35779098	8	67	theme	metabolites	1205:1215	arg1	levels					1185:1190	the levels	1181:1190	the levels of microbial metabolites such as unsaturated fatty acids and bile acids	1181:1262	In addition, it regulated the levels of microbial metabolites such as unsaturated fatty acids and bile acids.
35779098	6	68	theme	Alistipes	728:736	arg1	abundance					665:673	the abundance	661:673	the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella	661:752	PB supplementation significantly increased the abundance of Bifidobacterium, Parabacteroides, Alloprevotella, Alistipes, and Dubosiella, and decreased that of Robinsoniella, Blautia, Lachnoclostridium, Coprobacillus, Hungatella, Erysipelatoclostridium, Helicobacter, Clostridium sensu stricto 1, Enterococcus, and Akkermansia compared to that in the cefixime administration (CEF) group.
35779098	0	69	theme	mouse	81:85	arg1	model					87:91	a mouse model	79:91	a mouse model	79:91	Restoration of cefixime-induced gut microbiota changes by a prebiotic blend in a mouse model.
36161010	9	0	theme	Global	1644:1649	arg1	Health					1651:1656	Global Health	1644:1656	Global Health	1644:1656	These data represent an important step toward establishing Nicotiana tabacum as a biologics platform for Global Health.
36161010	6	1	theme	FucT	1089:1092	arg1	regions					1069:1075	three conserved regions	1053:1075	three conserved regions of the four FucT and two XylT genes	1053:1111	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	4	2	theme	Nicotiana	686:694	arg1	tabacum					696:702	Nicotiana tabacum	686:702	Nicotiana tabacum	686:702	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	1	3	theme	modern	190:195	arg1	biologics					197:205	modern biologics	190:205	modern biologics	190:205	Molecular pharming in plants offers exciting possibilities to address global access to modern biologics.
36161010	6	4	theme	SR-1	909:912	arg1	FucT					978:981	FucT	978:981	FucT	978:981	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	6	4	theme	SR-1	909:912	arg1	XylT					941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	909:975	XylT	941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	2	5	theme	α	309:309	arg1	-fucose					315:321	core α(1,3)-fucose	304:321	core α(1,3)-fucose	304:321	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	6	6	theme	-fucosyltransferase	957:975	arg1	lines					993:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines	909:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines	909:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	7	theme	soluble	1276:1282	arg1	protein					1284:1290	total soluble protein	1270:1290	total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants	1270:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	9	8	theme	important	1563:1571	arg1	step					1573:1576	an important step	1560:1576	an important step toward establishing Nicotiana tabacum as a biologics platform for Global Health	1560:1656	These data represent an important step toward establishing Nicotiana tabacum as a biologics platform for Global Health.
36161010	6	9	theme	1,3	953:955	arg1	-fucosyltransferase					957:975	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	909:975	-fucosyltransferase	957:975	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	10	theme	broadly	1418:1424	arg1	VRC01					1409:1413	recombinantly produced VRC01	1386:1413	recombinantly produced VRC01	1386:1413	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	10	theme	broadly	1418:1424	arg1	antibody					1456:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	6	11	theme	α	951:951	arg1	-fucosyltransferase					957:975	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	909:975	-fucosyltransferase	957:975	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	12	theme	anti-HIV-1	1439:1448	arg1	VRC01					1409:1413	recombinantly produced VRC01	1386:1413	recombinantly produced VRC01	1386:1413	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	12	theme	anti-HIV-1	1439:1448	arg1	antibody					1456:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	4	13	theme	choice	731:736	arg1	crop					723:726	the crop	719:726	the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries	719:872	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	3	14	theme	Nicotiana	599:607	arg1	benthamiana					609:619	Nicotiana benthamiana	599:619	Nicotiana benthamiana	599:619	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	4	15	theme	recombinant	742:752	arg1	pharmaceuticals					762:776	recombinant protein pharmaceuticals	742:776	recombinant protein pharmaceuticals required at massive scale	742:802	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	3	16	with	structures	455:464	arg1	changes					474:480	no changes	471:480	no changes in plant phenotype, growth, or recombinant protein expression levels	471:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	6	17	theme	multiplex	1017:1025	arg1	editing					1034:1040	CRISPR/Cas9 multiplex genome editing	1005:1040	CRISPR/Cas9 multiplex genome editing	1005:1040	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	0	18	theme	molecular	65:73	arg1	pharming					75:82	molecular pharming	65:82	molecular pharming using CRISPR/Cas9	65:100	Engineering the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9.
36161010	6	19	theme	knockout	984:991	arg1	lines					993:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines	909:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines	909:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	20	theme	targeted	1491:1498	arg1	regions					1500:1506	targeted regions	1491:1506	targeted regions of the putative transformants	1491:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	21	theme	α	1341:1341	arg1	-fucose					1347:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	2	22	theme	plant-derived	374:386	arg1	proteins					388:395	plant-derived proteins	374:395	plant-derived proteins	374:395	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	8	23	theme	putative	1515:1522	arg1	transformants					1524:1536	the putative transformants	1511:1536	the putative transformants	1511:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	7	24	theme	N-glycan	1173:1180	arg1	structures					1182:1191	non-human N-glycan structures	1163:1191	non-human N-glycan structures	1163:1191	These two enzymes are responsible for generating non-human N-glycan structures.
36161010	1	25	theme	Molecular	103:111	arg1	pharming					113:120	Molecular pharming	103:120	Molecular pharming in plants	103:130	Molecular pharming in plants offers exciting possibilities to address global access to modern biologics.
36161010	6	26	theme	conserved	1059:1067	arg1	regions					1069:1075	three conserved regions	1053:1075	three conserved regions of the four FucT and two XylT genes	1053:1111	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	27	theme	mass	1356:1359	arg1	analysis					1374:1381	mass spectrometry analysis	1356:1381	mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody	1356:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	0	28	theme	N-glycosylation	16:30	arg1	pathway					32:38	the N-glycosylation pathway	12:38	the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9	12:100	Engineering the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9.
36161010	3	29	theme	expression	533:542	arg1	levels					544:549	recombinant protein expression levels	513:549	recombinant protein expression levels	513:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	3	30	theme	Successful	398:407	arg1	approaches					426:435	Successful glycoengineering approaches	398:435	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels	398:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	4	31	theme	developed	854:862	arg1	countries					864:872	less developed countries	849:872	less developed countries	849:872	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	9	32	theme	Nicotiana	1598:1606	arg1	tabacum					1608:1614	Nicotiana tabacum	1598:1614	Nicotiana tabacum	1598:1614	These data represent an important step toward establishing Nicotiana tabacum as a biologics platform for Global Health.
36161010	9	32	theme	Nicotiana	1598:1606	arg1	platform					1631:1638	a biologics platform	1619:1638	a biologics platform for Global Health	1619:1656	These data represent an important step toward establishing Nicotiana tabacum as a biologics platform for Global Health.
36161010	3	33	theme	human-like	444:453	arg1	structures					455:464	human-like structures	444:464	human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels	444:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	0	34	theme	tabacum	53:59	arg1	pathway					32:38	the N-glycosylation pathway	12:38	the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9	12:100	Engineering the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9.
36161010	8	35	theme	VRC01	1409:1413	arg1	-fucose					1347:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	35	theme	VRC01	1409:1413	arg1	sequencing					1477:1486	Sanger sequencing	1470:1486	Sanger sequencing of targeted regions of the putative transformants	1470:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	35	theme	VRC01	1409:1413	arg1	analysis					1374:1381	mass spectrometry analysis	1356:1381	mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody	1356:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	3	36	theme	plant	485:489	arg1	phenotype					491:499	plant phenotype	485:499	plant phenotype	485:499	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	9	37	theme	biologics	1621:1629	arg1	tabacum					1608:1614	Nicotiana tabacum	1598:1614	Nicotiana tabacum	1598:1614	These data represent an important step toward establishing Nicotiana tabacum as a biologics platform for Global Health.
36161010	9	37	theme	biologics	1621:1629	arg1	platform					1631:1638	a biologics platform	1619:1638	a biologics platform for Global Health	1619:1656	These data represent an important step toward establishing Nicotiana tabacum as a biologics platform for Global Health.
36161010	6	38	theme	XylT	1102:1105	arg1	genes					1107:1111	the four FucT and two XylT genes	1080:1111	genes	1107:1111	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	4	39	theme	Such	622:625	arg1	engineering					627:637	Such engineering	622:637	Such engineering of N-glycosylation	622:656	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	3	40	theme	recombinant	513:523	arg1	levels					544:549	recombinant protein expression levels	513:549	recombinant protein expression levels	513:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	5	41	theme	tabacum	897:903	arg1	cv					905:906	N. tabacum cv	894:906	N. tabacum cv	894:906	Here, we generated N. tabacum cv.
36161010	6	42	theme	XylT	941:944	arg1	lines					993:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines	909:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines	909:997	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	6	43	theme	-xylosyltransferase	920:938	arg1	FucT					978:981	FucT	978:981	FucT	978:981	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	6	43	theme	-xylosyltransferase	920:938	arg1	XylT					941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	909:975	XylT	941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	2	44	theme	-xylose	292:298	arg1	presence					274:281	the presence	270:281	the presence of β(1,2)-xylose and core α(1,3)-fucose	270:321	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	6	45	theme	1,2	916:918	arg1	FucT					978:981	FucT	978:981	FucT	978:981	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	6	45	theme	1,2	916:918	arg1	XylT					941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	909:975	XylT	941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	2	46	theme	1,2	288:290	arg1	-xylose					292:298	β(1,2)-xylose	286:298	β(1,2)-xylose	286:298	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	5	47	theme	N.	894:895	arg1	tabacum					897:903	N. tabacum	894:903	N. tabacum cv	894:906	Here, we generated N. tabacum cv.
36161010	2	48	from	differences	217:227	arg1	pathway					252:258	the N-glycosylation pathway	232:258	the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose	232:321	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	6	49	theme	β	914:914	arg1	FucT					978:981	FucT	978:981	FucT	978:981	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	6	49	theme	β	914:914	arg1	XylT					941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase	909:975	XylT	941:944	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	50	theme	transformants	1235:1247	arg1	knockout					1223:1230	knockout	1223:1230	knockout	1223:1230	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	3	51	theme	Arabidopsis	574:584	arg1	thaliana					586:593	Arabidopsis thaliana	574:593	Arabidopsis thaliana	574:593	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	2	52	theme	core	304:307	arg1	1,3					311:313	1,3	311:313	1,3	311:313	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	2	52	theme	core	304:307	arg1	α					309:309	core α	304:309	core α(1,3)-fucose	304:321	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	8	53	theme	neutralizing	1426:1437	arg1	VRC01					1409:1413	recombinantly produced VRC01	1386:1413	recombinantly produced VRC01	1386:1413	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	53	theme	neutralizing	1426:1437	arg1	antibody					1456:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	54	theme	total	1270:1274	arg1	protein					1284:1290	total soluble protein	1270:1290	total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants	1270:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	55	theme	hIgG1	1450:1454	arg1	VRC01					1409:1413	recombinantly produced VRC01	1386:1413	recombinantly produced VRC01	1386:1413	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	55	theme	hIgG1	1450:1454	arg1	antibody					1456:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	56	theme	protein	1284:1290	arg1	immunoblotting					1252:1265	immunoblotting	1252:1265	immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants	1252:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	2	57	theme	N-glycosylation	236:250	arg1	pathway					252:258	the N-glycosylation pathway	232:258	the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose	232:321	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	4	58	theme	technology	826:835	arg1	transfer					837:844	technology transfer	826:844	technology transfer to less developed countries	826:872	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	2	59	theme	β	286:286	arg1	-xylose					292:298	β(1,2)-xylose	286:298	β(1,2)-xylose	286:298	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	1	60	theme	exciting	139:146	arg1	possibilities					148:160	exciting possibilities	139:160	exciting possibilities	139:160	Molecular pharming in plants offers exciting possibilities to address global access to modern biologics.
36161010	6	61	theme	genome	1027:1032	arg1	editing					1034:1040	CRISPR/Cas9 multiplex genome editing	1005:1040	CRISPR/Cas9 multiplex genome editing	1005:1040	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	62	theme	Sanger	1470:1475	arg1	sequencing					1477:1486	Sanger sequencing	1470:1486	Sanger sequencing of targeted regions of the putative transformants	1470:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	63	theme	β	1318:1318	arg1	1,3					1343:1345	1,3	1343:1345	1,3	1343:1345	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	63	theme	β	1318:1318	arg1	-xylose					1324:1330	β(1,2)-xylose	1318:1330	β(1,2)-xylose	1318:1330	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	6	64	theme	CRISPR/Cas9	1005:1015	arg1	editing					1034:1040	CRISPR/Cas9 multiplex genome editing	1005:1040	CRISPR/Cas9 multiplex genome editing	1005:1040	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	65	theme	1,2	1320:1322	arg1	1,3					1343:1345	1,3	1343:1345	1,3	1343:1345	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	65	theme	1,2	1320:1322	arg1	-xylose					1324:1330	β(1,2)-xylose	1318:1330	β(1,2)-xylose	1318:1330	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	1	66	theme	global	173:178	arg1	access					180:185	global access	173:185	global access to modern biologics	173:205	Molecular pharming in plants offers exciting possibilities to address global access to modern biologics.
36161010	8	67	theme	regions	1500:1506	arg1	-fucose					1347:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	67	theme	regions	1500:1506	arg1	sequencing					1477:1486	Sanger sequencing	1470:1486	Sanger sequencing of targeted regions of the putative transformants	1470:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	67	theme	regions	1500:1506	arg1	analysis					1374:1381	mass spectrometry analysis	1356:1381	mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody	1356:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	68	theme	-xylose	1324:1330	arg1	-fucose					1347:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	β(1,2)-xylose and core α(1,3)-fucose	1318:1353	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	69	theme	core	1336:1339	arg1	α					1341:1341	core α	1336:1341	core α	1336:1341	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	4	70	theme	massive	790:796	arg1	scale					798:802	massive scale	790:802	massive scale	790:802	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	8	71	theme	transformants	1524:1536	arg1	regions					1500:1506	targeted regions	1491:1506	targeted regions of the putative transformants	1491:1536	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	7	72	theme	non-human	1163:1171	arg1	structures					1182:1191	non-human N-glycan structures	1163:1191	non-human N-glycan structures	1163:1191	These two enzymes are responsible for generating non-human N-glycan structures.
36161010	4	73	theme	protein	754:760	arg1	pharmaceuticals					762:776	recombinant protein pharmaceuticals	742:776	recombinant protein pharmaceuticals required at massive scale	742:802	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	0	74	theme	Nicotiana	43:51	arg1	tabacum					53:59	Nicotiana tabacum	43:59	Nicotiana tabacum	43:59	Engineering the N-glycosylation pathway of Nicotiana tabacum for molecular pharming using CRISPR/Cas9.
36161010	3	75	theme	glycoengineering	409:424	arg1	approaches					426:435	Successful glycoengineering approaches	398:435	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels	398:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	2	76	link	plant-derived	374:386	arg1	proteins					388:395	plant-derived proteins	374:395	plant-derived proteins	374:395	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	8	77	theme	spectrometry	1361:1372	arg1	analysis					1374:1381	mass spectrometry analysis	1356:1381	mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody	1356:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	2	78	theme	proteins	388:395	arg1	activity					334:341	activity	334:341	activity	334:341	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	2	78	theme	proteins	388:395	arg1	immunogenicity					356:369	immunogenicity	356:369	immunogenicity	356:369	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	2	78	theme	proteins	388:395	arg1	potency					344:350	potency	344:350	potency	344:350	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	3	79	from	changes	474:480	arg1	growth					502:507	growth	502:507	growth	502:507	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	3	79	from	changes	474:480	arg1	levels					544:549	recombinant protein expression levels	513:549	recombinant protein expression levels	513:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	3	79	from	changes	474:480	arg1	phenotype					491:499	plant phenotype	485:499	plant phenotype	485:499	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36161010	2	80	theme	-fucose	315:321	arg1	presence					274:281	the presence	270:281	the presence of β(1,2)-xylose and core α(1,3)-fucose	270:321	However, differences in the N-glycosylation pathway including the presence of β(1,2)-xylose and core α(1,3)-fucose can affect activity, potency and immunogenicity of plant-derived proteins.
36161010	1	81	from	pharming	113:120	arg1	plants					125:130	plants	125:130	plants	125:130	Molecular pharming in plants offers exciting possibilities to address global access to modern biologics.
36161010	6	82	theme	genes	1107:1111	arg1	regions					1069:1075	three conserved regions	1053:1075	three conserved regions of the four FucT and two XylT genes	1053:1111	SR-1 β(1,2)-xylosyltransferase (XylT) and α(1,3)-fucosyltransferase (FucT) knockout lines using CRISPR/Cas9 multiplex genome editing, targeting three conserved regions of the four FucT and two XylT genes.
36161010	8	83	theme	produced	1400:1407	arg1	VRC01					1409:1413	recombinantly produced VRC01	1386:1413	recombinantly produced VRC01	1386:1413	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	8	83	theme	produced	1400:1407	arg1	antibody					1456:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	a broadly neutralizing anti-HIV-1 hIgG1 antibody	1416:1463	We confirmed full functional knockout of transformants by immunoblotting of total soluble protein by antibodies recognizing β(1,2)-xylose and core α(1,3)-fucose, mass spectrometry analysis of recombinantly produced VRC01, a broadly neutralizing anti-HIV-1 hIgG1 antibody, and Sanger sequencing of targeted regions of the putative transformants.
36161010	4	84	theme	N-glycosylation	642:656	arg1	engineering					627:637	Such engineering	622:637	Such engineering of N-glycosylation	622:656	Such engineering of N-glycosylation would also be desirable for Nicotiana tabacum, which remains the crop of choice for recombinant protein pharmaceuticals required at massive scale and for manufacturing technology transfer to less developed countries.
36161010	3	85	theme	protein	525:531	arg1	levels					544:549	recombinant protein expression levels	513:549	recombinant protein expression levels	513:549	Successful glycoengineering approaches toward human-like structures with no changes in plant phenotype, growth, or recombinant protein expression levels have been reported for Arabidopsis thaliana and Nicotiana benthamiana.
36145255	0	0	theme	LPS	120:122	arg1	Model					131:135	LPS Murine Model	120:135	LPS Murine Model	120:135	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model.
36145255	8	1	theme	neural	1564:1569	arg1	proliferation					1582:1594	neural progenitor proliferation	1564:1594	neural progenitor proliferation	1564:1594	To conclude, we found that tested DS suppresses the neuroinflammatory response by reducing glial activation, positively regulates neural progenitor proliferation, and attenuates hippocampal-dependent memory impairment.
36145255	2	2	from	process	351:357	arg1	neuroinflammation					370:386	chronic neuroinflammation	362:386	chronic neuroinflammation	362:386	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	8	3	theme	progenitor	1571:1580	arg1	proliferation					1582:1594	neural progenitor proliferation	1564:1594	neural progenitor proliferation	1564:1594	To conclude, we found that tested DS suppresses the neuroinflammatory response by reducing glial activation, positively regulates neural progenitor proliferation, and attenuates hippocampal-dependent memory impairment.
36145255	7	4	theme	range	1321:1325	arg1	production					1305:1314	the production	1301:1314	the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition	1301:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	3	5	theme	active	581:586	arg1	mediators					594:602	highly active lipid mediators	574:602	highly active lipid mediators	574:602	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	8	6	theme	neuroinflammatory	1486:1502	arg1	response					1504:1511	the neuroinflammatory response	1482:1511	the neuroinflammatory response	1482:1511	To conclude, we found that tested DS suppresses the neuroinflammatory response by reducing glial activation, positively regulates neural progenitor proliferation, and attenuates hippocampal-dependent memory impairment.
36145255	5	7	theme	anti-inflammatory	1078:1094	arg1	activity					1096:1103	a pronounced anti-inflammatory activity	1065:1103	a pronounced anti-inflammatory activity of DS	1065:1109	By detecting the production of pro-inflammatory cytokines and glial markers, a pronounced anti-inflammatory activity of DS was demonstrated both in vitro and in vivo.
36145255	2	8	theme	resolution	340:349	arg1	process					351:357	the resolution process	336:357	the resolution process in chronic neuroinflammation	336:386	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	3	9	from	activity	627:634	arg1	processes					652:660	inflammatory processes	639:660	inflammatory processes	639:660	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	0	10	theme	Murine	124:129	arg1	Model					131:135	LPS Murine Model	120:135	LPS Murine Model	120:135	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model.
36145255	7	11	theme	well-documented	1340:1354	arg1	activity					1374:1381	well-documented anti-inflammatory activity	1340:1381	well-documented anti-inflammatory activity in response to the administered lipid composition	1340:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	5	12	theme	markers	1056:1062	arg1	production					1005:1014	the production	1001:1014	the production of pro-inflammatory cytokines and glial markers	1001:1062	By detecting the production of pro-inflammatory cytokines and glial markers, a pronounced anti-inflammatory activity of DS was demonstrated both in vitro and in vivo.
36145255	4	13	theme	effects	790:796	arg1	study					777:781	a study	775:781	a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes	775:985	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	4	14	theme	Berryteuthis	906:917	arg1	magister					919:926	squid Berryteuthis magister	900:926	squid Berryteuthis magister	900:926	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	7	15	from	activity	1374:1381	arg1	response					1386:1393	response	1386:1393	response to the administered lipid composition	1386:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	3	16	theme	N-acylethanolamine	515:532	arg1	derivatives					540:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	4	17	theme	squid	900:904	arg1	magister					919:926	squid Berryteuthis magister	900:926	squid Berryteuthis magister	900:926	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	4	18	link	acid-derived	867:878	arg1	NAEs					880:883	fatty acid-derived NAEs	861:883	fatty acid-derived NAEs	861:883	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	1	19	theme	neurological	206:217	arg1	pathogenesis					185:196	the pathogenesis	181:196	the pathogenesis of most neurological and neurodegenerative diseases	181:248	Neuroinflammation plays a critical role in the pathogenesis of most neurological and neurodegenerative diseases and therefore represents a potential therapeutic target.
36145255	0	20	theme	Acid-Derived	6:17	arg1	Supplementation					47:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model.
36145255	7	21	from	increase	1289:1296	arg1	production					1305:1314	the production	1301:1314	the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition	1301:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	7	22	theme	administered	1402:1413	arg1	composition					1421:1431	the administered lipid composition	1398:1431	the administered lipid composition	1398:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	2	23	from	strategy	408:415	arg1	regard					315:320	this regard	310:320	this regard	310:320	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	2	24	theme	cognitive	434:442	arg1	consequences					444:455	the cognitive consequences	430:455	the cognitive consequences of neuropathology and generalized inflammatory processes	430:512	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	0	25	theme	Fatty	0:4	arg1	Supplementation					47:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model.
36145255	8	26	theme	memory	1634:1639	arg1	impairment					1641:1650	hippocampal-dependent memory impairment	1612:1650	hippocampal-dependent memory impairment	1612:1650	To conclude, we found that tested DS suppresses the neuroinflammatory response by reducing glial activation, positively regulates neural progenitor proliferation, and attenuates hippocampal-dependent memory impairment.
36145255	2	27	theme	chronic	362:368	arg1	neuroinflammation					370:386	chronic neuroinflammation	362:386	chronic neuroinflammation	362:386	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	1	28	theme	neurodegenerative	223:239	arg1	diseases					241:248	neurodegenerative diseases	223:248	neurodegenerative diseases	223:248	Neuroinflammation plays a critical role in the pathogenesis of most neurological and neurodegenerative diseases and therefore represents a potential therapeutic target.
36145255	0	29	theme	Dietary	39:45	arg1	Supplementation					47:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model.
36145255	4	30	theme	dietary	808:814	arg1	DS					828:829	DS	828:829	DS	828:829	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	4	30	theme	dietary	808:814	arg1	supplement					816:825	dietary supplement	808:825	dietary supplement (DS)	808:830	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	6	31	theme	memory	1240:1245	arg1	deterioration					1247:1259	memory deterioration	1240:1259	memory deterioration	1240:1259	DS administration reversed the LPS-induced reduction in hippocampal neurogenesis and memory deterioration.
36145255	5	32	theme	cytokines	1036:1044	arg1	production					1005:1014	the production	1001:1014	the production of pro-inflammatory cytokines and glial markers	1001:1062	By detecting the production of pro-inflammatory cytokines and glial markers, a pronounced anti-inflammatory activity of DS was demonstrated both in vitro and in vivo.
36145255	2	33	theme	inflammatory	491:502	arg1	processes					504:512	generalized inflammatory processes	479:512	generalized inflammatory processes	479:512	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	2	34	theme	processes	504:512	arg1	consequences					444:455	the cognitive consequences	430:455	the cognitive consequences of neuropathology and generalized inflammatory processes	430:512	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	5	35	theme	pro-inflammatory	1019:1034	arg1	cytokines					1036:1044	pro-inflammatory cytokines	1019:1044	pro-inflammatory cytokines	1019:1044	By detecting the production of pro-inflammatory cytokines and glial markers, a pronounced anti-inflammatory activity of DS was demonstrated both in vitro and in vivo.
36145255	3	36	theme	consequences	736:747	arg1	suppression					695:705	the suppression	691:705	the suppression of neuroinflammation and its consequences	691:747	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	3	37	theme	inflammatory	639:650	arg1	processes					652:660	inflammatory processes	639:660	inflammatory processes	639:660	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	2	38	theme	generalized	479:489	arg1	processes					504:512	generalized inflammatory processes	479:512	generalized inflammatory processes	479:512	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	7	39	theme	lipid	1415:1419	arg1	composition					1421:1431	the administered lipid composition	1398:1431	the administered lipid composition	1398:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	5	40	theme	glial	1050:1054	arg1	markers					1056:1062	glial markers	1050:1062	glial markers	1050:1062	By detecting the production of pro-inflammatory cytokines and glial markers, a pronounced anti-inflammatory activity of DS was demonstrated both in vitro and in vivo.
36145255	7	41	theme	anti-inflammatory	1356:1372	arg1	activity					1374:1381	well-documented anti-inflammatory activity	1340:1381	well-documented anti-inflammatory activity in response to the administered lipid composition	1340:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	5	42	theme	DS	1108:1109	arg1	activity					1096:1103	a pronounced anti-inflammatory activity	1065:1103	a pronounced anti-inflammatory activity of DS	1065:1109	By detecting the production of pro-inflammatory cytokines and glial markers, a pronounced anti-inflammatory activity of DS was demonstrated both in vitro and in vivo.
36145255	7	43	with	NAEs	1330:1333	arg1	activity					1374:1381	well-documented anti-inflammatory activity	1340:1381	well-documented anti-inflammatory activity in response to the administered lipid composition	1340:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	3	44	theme	NAE	535:537	arg1	derivatives					540:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	7	45	theme	LC-MS	1262:1266	arg1	analysis					1268:1275	LC-MS analysis	1262:1275	LC-MS analysis	1262:1275	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	3	46	contain	possess	605:611	arg2	activity					627:634	pro-resolving activity	613:634	pro-resolving activity in inflammatory processes	613:660	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	3	46	contain	possess	605:611	arg1	derivatives					540:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	4	47	theme	NAEs	880:883	arg1	composition					846:856	a composition	844:856	a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister	844:926	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	2	48	theme	neuropathology	460:473	arg1	consequences					444:455	the cognitive consequences	430:455	the cognitive consequences of neuropathology and generalized inflammatory processes	430:512	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	4	49	contain	containing	833:842	arg2	composition					846:856	a composition	844:856	a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister	844:926	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	4	49	contain	containing	833:842	arg1	DS					828:829	DS	828:829	DS	828:829	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	4	49	contain	containing	833:842	arg1	supplement					816:825	dietary supplement	808:825	dietary supplement (DS)	808:830	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	4	50	theme	memory	970:975	arg1	processes					977:985	the hippocampal neuroinflammatory and memory processes	932:985	processes	977:985	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	7	51	theme	NAEs	1330:1333	arg1	range					1321:1325	a range	1319:1325	a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition	1319:1431	LC-MS analysis revealed an increase in the production of a range of NAEs with well-documented anti-inflammatory activity in response to the administered lipid composition.
36145255	6	52	theme	hippocampal	1211:1221	arg1	neurogenesis					1223:1234	hippocampal neurogenesis	1211:1234	hippocampal neurogenesis	1211:1234	DS administration reversed the LPS-induced reduction in hippocampal neurogenesis and memory deterioration.
36145255	2	53	theme	effective	398:406	arg1	strategy					408:415	an effective strategy	395:415	an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes	395:512	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	3	54	theme	fatty	555:559	arg1	acids					561:565	fatty acids	555:565	fatty acids	555:565	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	8	55	theme	hippocampal-dependent	1612:1632	arg1	impairment					1641:1650	hippocampal-dependent memory impairment	1612:1650	hippocampal-dependent memory impairment	1612:1650	To conclude, we found that tested DS suppresses the neuroinflammatory response by reducing glial activation, positively regulates neural progenitor proliferation, and attenuates hippocampal-dependent memory impairment.
36145255	3	56	theme	promising	670:678	arg1	agents					680:685	promising agents	670:685	promising agents for the suppression of neuroinflammation and its consequences	670:747	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	4	57	theme	fatty	861:865	arg1	NAEs					880:883	fatty acid-derived NAEs	861:883	fatty acid-derived NAEs	861:883	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	5	58	theme	pronounced	1067:1076	arg1	activity					1096:1103	a pronounced anti-inflammatory activity	1065:1103	a pronounced anti-inflammatory activity of DS	1065:1109	By detecting the production of pro-inflammatory cytokines and glial markers, a pronounced anti-inflammatory activity of DS was demonstrated both in vitro and in vivo.
36145255	1	59	theme	critical	164:171	arg1	role					173:176	a critical role	162:176	a critical role	162:176	Neuroinflammation plays a critical role in the pathogenesis of most neurological and neurodegenerative diseases and therefore represents a potential therapeutic target.
36145255	8	60	theme	tested	1461:1466	arg1	DS					1468:1469	tested DS	1461:1469	tested DS	1461:1469	To conclude, we found that tested DS suppresses the neuroinflammatory response by reducing glial activation, positively regulates neural progenitor proliferation, and attenuates hippocampal-dependent memory impairment.
36145255	3	61	theme	neuroinflammation	710:726	arg1	suppression					695:705	the suppression	691:705	the suppression of neuroinflammation and its consequences	691:747	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	3	62	theme	acids	561:565	arg1	derivatives					540:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives	515:550	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	1	63	theme	potential	277:285	arg1	target					299:304	a potential therapeutic target	275:304	a potential therapeutic target	275:304	Neuroinflammation plays a critical role in the pathogenesis of most neurological and neurodegenerative diseases and therefore represents a potential therapeutic target.
36145255	2	64	with	deal	420:423	arg1	consequences					444:455	the cognitive consequences	430:455	the cognitive consequences of neuropathology and generalized inflammatory processes	430:512	In this regard, accelerating the resolution process in chronic neuroinflammation may be an effective strategy to deal with the cognitive consequences of neuropathology and generalized inflammatory processes.
36145255	4	65	theme	acid-derived	867:878	arg1	NAEs					880:883	fatty acid-derived NAEs	861:883	fatty acid-derived NAEs	861:883	This paper is devoted to a study of the effects played by dietary supplement (DS), containing a composition of fatty acid-derived NAEs, obtained from squid Berryteuthis magister, on the hippocampal neuroinflammatory and memory processes.
36145255	1	66	theme	diseases	241:248	arg1	pathogenesis					185:196	the pathogenesis	181:196	the pathogenesis of most neurological and neurodegenerative diseases	181:248	Neuroinflammation plays a critical role in the pathogenesis of most neurological and neurodegenerative diseases and therefore represents a potential therapeutic target.
36145255	3	67	theme	lipid	588:592	arg1	mediators					594:602	highly active lipid mediators	574:602	highly active lipid mediators	574:602	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	6	68	theme	DS	1155:1156	arg1	administration					1158:1171	DS administration	1155:1171	DS administration	1155:1171	DS administration reversed the LPS-induced reduction in hippocampal neurogenesis and memory deterioration.
36145255	1	69	theme	therapeutic	287:297	arg1	target					299:304	a potential therapeutic target	275:304	a potential therapeutic target	275:304	Neuroinflammation plays a critical role in the pathogenesis of most neurological and neurodegenerative diseases and therefore represents a potential therapeutic target.
36145255	0	70	theme	Cognitive	96:104	arg1	Impairment					106:115	Cognitive Impairment	96:115	Cognitive Impairment	96:115	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model.
36145255	3	71	theme	pro-resolving	613:625	arg1	activity					627:634	pro-resolving activity	613:634	pro-resolving activity in inflammatory processes	613:660	N-acylethanolamine (NAE) derivatives of fatty acids, being highly active lipid mediators, possess pro-resolving activity in inflammatory processes and are promising agents for the suppression of neuroinflammation and its consequences.
36145255	0	72	theme	N-acylethanolamines	19:37	arg1	Supplementation					47:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation	0:61	Fatty Acid-Derived N-acylethanolamines Dietary Supplementation Attenuates Neuroinflammation and Cognitive Impairment in LPS Murine Model.
36145255	6	73	from	reduction	1198:1206	arg1	neurogenesis					1223:1234	hippocampal neurogenesis	1211:1234	hippocampal neurogenesis	1211:1234	DS administration reversed the LPS-induced reduction in hippocampal neurogenesis and memory deterioration.
36145255	6	73	from	reduction	1198:1206	arg1	deterioration					1247:1259	memory deterioration	1240:1259	memory deterioration	1240:1259	DS administration reversed the LPS-induced reduction in hippocampal neurogenesis and memory deterioration.
36145255	6	74	theme	LPS-induced	1186:1196	arg1	reduction					1198:1206	the LPS-induced reduction	1182:1206	the LPS-induced reduction in hippocampal neurogenesis and memory deterioration	1182:1259	DS administration reversed the LPS-induced reduction in hippocampal neurogenesis and memory deterioration.
36145255	8	75	theme	glial	1525:1529	arg1	activation					1531:1540	glial activation	1525:1540	glial activation	1525:1540	To conclude, we found that tested DS suppresses the neuroinflammatory response by reducing glial activation, positively regulates neural progenitor proliferation, and attenuates hippocampal-dependent memory impairment.
35673929	4	0	theme	composition	833:843	arg1	terms					815:819	terms	815:819	terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM	815:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	9	1	theme	failed	1637:1642	arg1	attempt					1644:1650	a failed attempt	1635:1650	a failed attempt to stabilize and/or restore the tissue	1635:1689	At the late stage, we observe a failed attempt to stabilize and/or restore the tissue, yielding significant degradation of the supramolecular assemblies.
35673929	6	2	theme	fibrils	1238:1244	arg1	level					1209:1213	the level	1205:1213	the level of individual collagen fibrils	1205:1244	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	2	3	theme	musculoskeletal	365:379	arg1	development					313:323	the development	309:323	the development of osteoarthritis (OA)	309:346	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	2	3	theme	musculoskeletal	365:379	arg1	disorder					381:388	the most common musculoskeletal disorder	349:388	the most common musculoskeletal disorder	349:388	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	1	4	theme	aggrecan	281:288	arg1	aggregates					290:299	interfibrillar aggrecan aggregates	266:299	interfibrillar aggrecan aggregates	266:299	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	4	5	theme	morphological	849:861	arg1	properties					878:887	morphological and mechanical properties	849:887	morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM	849:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	6	6	theme	force	1158:1162	arg1	AFM					1176:1178	AFM	1176:1178	AFM	1176:1178	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	6	6	theme	force	1158:1162	arg1	microscopy					1164:1173	atomic force microscopy	1151:1173	atomic force microscopy (AFM)	1151:1179	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	6	7	theme	individual	1218:1227	arg1	fibrils					1238:1244	individual collagen fibrils	1218:1244	individual collagen fibrils	1218:1244	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	7	8	theme	OA	1416:1417	arg1	progress					1419:1426	the OA progress	1412:1426	the OA progress	1412:1426	Taken together, these nanoscale characterization studies reveal the existence of two stages in the OA progress.
35673929	9	9	theme	late	1612:1615	arg1	stage					1617:1621	the late stage	1608:1621	the late stage	1608:1621	At the late stage, we observe a failed attempt to stabilize and/or restore the tissue, yielding significant degradation of the supramolecular assemblies.
35673929	1	10	theme	three-dimensional	187:203	arg1	matrix					146:151	The extracellular matrix	128:151	The extracellular matrix (ECM) of articular cartilage	128:180	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	1	10	theme	three-dimensional	187:203	arg1	network					205:211	a three-dimensional network	185:211	a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates	185:299	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	7	11	theme	nanoscale	1339:1347	arg1	studies					1366:1372	these nanoscale characterization studies	1333:1372	these nanoscale characterization studies	1333:1372	Taken together, these nanoscale characterization studies reveal the existence of two stages in the OA progress.
35673929	2	12	theme	osteoarthritis	328:341	arg1	development					313:323	the development	309:323	the development of osteoarthritis (OA)	309:346	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	2	12	theme	osteoarthritis	328:341	arg1	disorder					381:388	the most common musculoskeletal disorder	349:388	the most common musculoskeletal disorder	349:388	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	15	13	theme	catabolic	2564:2572	arg1	processes					2574:2582	catabolic processes	2564:2582	catabolic processes	2564:2582	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	4	14	theme	supramolecular	905:918	arg1	assemblies					920:929	supramolecular assemblies	905:929	supramolecular assemblies constituting the cartilage ECM	905:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	12	15	theme	healthy	2063:2069	arg1	cartilage					2071:2079	healthy cartilage	2063:2079	healthy cartilage	2063:2079	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	6	16	theme	spectroscopy	1270:1281	arg1	modes					1283:1287	both imaging and force spectroscopy modes	1247:1287	both imaging and force spectroscopy modes	1247:1287	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	5	17	theme	innovative	1046:1055	arg1	approach					1072:1079	an innovative methodological approach	1043:1079	an innovative methodological approach that provides the molecular composition of the ECM	1043:1130	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	9	18	theme	supramolecular	1732:1745	arg1	assemblies					1747:1756	the supramolecular assemblies	1728:1756	the supramolecular assemblies	1728:1756	At the late stage, we observe a failed attempt to stabilize and/or restore the tissue, yielding significant degradation of the supramolecular assemblies.
35673929	4	19	theme	mechanical	867:876	arg1	properties					878:887	morphological and mechanical properties	849:887	morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM	849:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	12	20	theme	chondrocytes	2014:2025	arg1	activities					2000:2009	the imbalanced activities	1985:2009	the imbalanced activities of chondrocytes	1985:2025	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	3	21	theme	pathological	587:598	arg1	remodelling					600:610	the pathological remodelling	583:610	the pathological remodelling of the ECM	583:621	While the molecular mechanisms involved in the pathological remodelling of the ECM are considered as decisive, they remain, however, not completely elucidated.
35673929	15	22	theme	curative	2420:2427	arg1	treatments					2429:2438	curative treatments	2420:2438	curative treatments	2420:2438	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	1	23	theme	extracellular	132:144	arg1	matrix					146:151	The extracellular matrix	128:151	The extracellular matrix (ECM) of articular cartilage	128:180	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	1	23	theme	extracellular	132:144	arg1	network					205:211	a three-dimensional network	185:211	a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates	185:299	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	1	23	theme	extracellular	132:144	arg1	ECM					154:156	ECM	154:156	ECM	154:156	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	15	24	theme	treatments	2429:2438	arg1	development					2405:2415	the development	2401:2415	the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes	2401:2582	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	14	25	theme	better	2275:2280	arg1	understanding					2282:2294	a better understanding	2273:2294	a better understanding of the mechanism by which OA is initiated and progresses in the cartilage ECM	2273:2372	These findings bring mechanistic insights leading to a better understanding of the mechanism by which OA is initiated and progresses in the cartilage ECM.
35673929	0	26	theme	matrix	74:79	arg1	remodelling					25:35	pathological remodelling	12:35	pathological remodelling of the human cartilage extracellular matrix in osteoarthritis	12:97	Deciphering pathological remodelling of the human cartilage extracellular matrix in osteoarthritis at the supramolecular level.
35673929	5	27	theme	molecular	1099:1107	arg1	composition					1109:1119	the molecular composition	1095:1119	the molecular composition of the ECM	1095:1130	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	3	28	theme	ECM	619:621	arg1	remodelling					600:610	the pathological remodelling	583:610	the pathological remodelling of the ECM	583:621	While the molecular mechanisms involved in the pathological remodelling of the ECM are considered as decisive, they remain, however, not completely elucidated.
35673929	4	29	dep	role	753:756	arg1	the					749:751	the	749:751	the	749:751	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	15	30	theme	metabolism	2498:2507	arg1	balance					2475:2481	the homeostatic balance	2459:2481	the homeostatic balance of chondrocyte metabolism	2459:2507	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	0	31	from	remodelling	25:35	arg1	osteoarthritis					84:97	osteoarthritis	84:97	osteoarthritis	84:97	Deciphering pathological remodelling of the human cartilage extracellular matrix in osteoarthritis at the supramolecular level.
35673929	15	32	theme	chondrocyte	2486:2496	arg1	metabolism					2498:2507	chondrocyte metabolism	2486:2507	chondrocyte metabolism	2486:2507	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	1	33	theme	articular	162:170	arg1	cartilage					172:180	articular cartilage	162:180	articular cartilage	162:180	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	4	34	theme	cartilage	948:956	arg1	ECM					958:960	the cartilage ECM	944:960	the cartilage ECM	944:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	8	35	theme	marked	1451:1456	arg1	increase					1458:1465	a marked increase	1449:1465	a marked increase in the aggrecan and collagen content	1449:1502	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	4	36	theme	relevant	720:727	arg1	way					729:731	a relevant way	718:731	a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM	718:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	2	37	theme	structural	445:454	arg1	changes					456:462	chemical and structural changes	432:462	chemical and structural changes that play a pivotal role in the initiation and the progress of the disease	432:537	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	8	38	theme	aggrecan	1474:1481	arg1	content					1496:1502	the aggrecan and collagen content	1470:1502	content	1496:1502	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	12	39	theme	collagenase	2131:2141	arg1	action					2103:2108	the combined action	2090:2108	the combined action of hyaluronidase and collagenase	2090:2141	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	2	40	theme	chemical	432:439	arg1	changes					456:462	chemical and structural changes	432:462	chemical and structural changes that play a pivotal role in the initiation and the progress of the disease	432:537	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	8	41	theme	collagen	1487:1494	arg1	content					1496:1502	the aggrecan and collagen content	1470:1502	content	1496:1502	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	4	42	theme	human	787:791	arg1	tissues					803:809	human cartilage tissues	787:809	human cartilage tissues	787:809	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	5	43	theme	ECM	1128:1130	arg1	composition					1109:1119	the molecular composition	1095:1119	the molecular composition of the ECM	1095:1130	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	5	44	theme	photoelectron	995:1007	arg1	XPS					1023:1025	XPS	1023:1025	XPS	1023:1025	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	5	44	theme	photoelectron	995:1007	arg1	spectroscopy					1009:1020	X-ray photoelectron spectroscopy	989:1020	X-ray photoelectron spectroscopy (XPS)	989:1026	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	5	45	used	used	984:987	arg2	we					981:982	we	981:982	we	981:982	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	0	46	theme	cartilage	50:58	arg1	matrix					74:79	the human cartilage extracellular matrix	40:79	the human cartilage extracellular matrix	40:79	Deciphering pathological remodelling of the human cartilage extracellular matrix in osteoarthritis at the supramolecular level.
35673929	1	47	theme	entangled	235:243	arg1	fibrils					254:260	entangled collagen fibrils	235:260	entangled collagen fibrils	235:260	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	2	48	dep	initiation	496:505	arg1	the					492:494	the	492:494	the	492:494	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	13	49	theme	OA	2207:2208	arg1	severity					2210:2217	high OA severity	2202:2217	high OA severity	2202:2217	This yields damage strictly analogous to that observed at high OA severity.
35673929	4	50	theme	progress	775:782	arg1	nature					762:767	nature	762:767	nature	762:767	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	4	50	theme	progress	775:782	arg1	role					753:756	role	753:756	role	753:756	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	14	51	theme	cartilage	2360:2368	arg1	ECM					2370:2372	the cartilage ECM	2356:2372	the cartilage ECM	2356:2372	These findings bring mechanistic insights leading to a better understanding of the mechanism by which OA is initiated and progresses in the cartilage ECM.
35673929	15	52	theme	reactions	2542:2550	arg1	control					2521:2527	the control	2517:2527	the control of enzymatic reactions involved in catabolic processes	2517:2582	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	8	53	from	increase	1458:1465	arg1	content					1496:1502	the aggrecan and collagen content	1470:1502	content	1496:1502	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	8	54	theme	chondrocyte	1544:1554	arg1	activity					1556:1563	the homeostatic chondrocyte activity	1528:1563	the homeostatic chondrocyte activity that tends to repair the cartilage ECM	1528:1602	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	10	55	theme	catabolic	1837:1845	arg1	events					1847:1852	catabolic events	1837:1852	catabolic events	1837:1852	This suggests an imbalance in the chondrocyte activity that turns in favor of catabolic events.
35673929	1	56	theme	interfibrillar	266:279	arg1	aggregates					290:299	interfibrillar aggrecan aggregates	266:299	interfibrillar aggrecan aggregates	266:299	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	8	57	theme	early	1436:1440	arg1	stage					1442:1446	the early stage	1432:1446	the early stage	1432:1446	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	4	58	theme	chemical	824:831	arg1	composition					833:843	chemical composition	824:843	chemical composition	824:843	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	6	59	theme	collagen	1229:1236	arg1	fibrils					1238:1244	individual collagen fibrils	1218:1244	individual collagen fibrils	1218:1244	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	6	60	theme	atomic	1151:1156	arg1	AFM					1176:1178	AFM	1176:1178	AFM	1176:1178	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	6	60	theme	atomic	1151:1156	arg1	microscopy					1164:1173	atomic force microscopy	1151:1173	atomic force microscopy (AFM)	1151:1179	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	2	61	theme	common	358:363	arg1	development					313:323	the development	309:323	the development of osteoarthritis (OA)	309:346	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	2	61	theme	common	358:363	arg1	disorder					381:388	the most common musculoskeletal disorder	349:388	the most common musculoskeletal disorder	349:388	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	1	62	theme	cartilage	172:180	arg1	matrix					146:151	The extracellular matrix	128:151	The extracellular matrix (ECM) of articular cartilage	128:180	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	1	62	theme	cartilage	172:180	arg1	network					205:211	a three-dimensional network	185:211	a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates	185:299	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	1	62	theme	cartilage	172:180	arg1	ECM					154:156	ECM	154:156	ECM	154:156	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	10	63	from	turns	1819:1823	arg1	favor					1828:1832	favor	1828:1832	favor	1828:1832	This suggests an imbalance in the chondrocyte activity that turns in favor of catabolic events.
35673929	7	64	theme	stages	1402:1407	arg1	existence					1385:1393	the existence	1381:1393	the existence of two stages in the OA progress	1381:1426	Taken together, these nanoscale characterization studies reveal the existence of two stages in the OA progress.
35673929	12	65	theme	cartilage	2071:2079	arg1	digestions					2049:2058	enzymatic digestions	2039:2058	enzymatic digestions of healthy cartilage	2039:2079	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	14	66	theme	mechanism	2303:2311	arg1	understanding					2282:2294	a better understanding	2273:2294	a better understanding of the mechanism by which OA is initiated and progresses in the cartilage ECM	2273:2372	These findings bring mechanistic insights leading to a better understanding of the mechanism by which OA is initiated and progresses in the cartilage ECM.
35673929	3	67	theme	molecular	550:558	arg1	mechanisms					560:569	the molecular mechanisms	546:569	the molecular mechanisms involved in the pathological remodelling of the ECM	546:621	While the molecular mechanisms involved in the pathological remodelling of the ECM are considered as decisive, they remain, however, not completely elucidated.
35673929	3	67	theme	molecular	550:558	arg1	decisive					641:648	decisive	641:648	decisive	641:648	While the molecular mechanisms involved in the pathological remodelling of the ECM are considered as decisive, they remain, however, not completely elucidated.
35673929	6	68	used	used	1146:1149	arg2	we					1143:1144	we	1143:1144	we	1143:1144	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	7	69	theme	characterization	1349:1364	arg1	studies					1366:1372	these nanoscale characterization studies	1333:1372	these nanoscale characterization studies	1333:1372	Taken together, these nanoscale characterization studies reveal the existence of two stages in the OA progress.
35673929	12	70	theme	enzymatic	2039:2047	arg1	digestions					2049:2058	enzymatic digestions	2039:2058	enzymatic digestions of healthy cartilage	2039:2079	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	2	71	theme	disease	531:537	arg1	initiation					496:505	initiation	496:505	initiation	496:505	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	2	71	theme	disease	531:537	arg1	progress					515:522	the progress	511:522	the progress	511:522	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	6	72	theme	force	1264:1268	arg1	spectroscopy					1270:1281	force spectroscopy	1264:1281	force spectroscopy	1264:1281	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	5	73	theme	methodological	1057:1070	arg1	approach					1072:1079	an innovative methodological approach	1043:1079	an innovative methodological approach that provides the molecular composition of the ECM	1043:1130	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	6	74	theme	imaging	1252:1258	arg1	modes					1283:1287	both imaging and force spectroscopy modes	1247:1287	both imaging and force spectroscopy modes	1247:1287	Moreover, we used atomic force microscopy (AFM) to probe the tissues at the level of individual collagen fibrils, both imaging and force spectroscopy modes being explored to this end.
35673929	13	75	located	observed	2190:2197	arg1	severity					2210:2217	high OA severity	2202:2217	high OA severity	2202:2217	This yields damage strictly analogous to that observed at high OA severity.
35673929	13	75	located	observed	2190:2197	arg2	that					2185:2188	that	2185:2188	that	2185:2188	This yields damage strictly analogous to that observed at high OA severity.
35673929	4	76	theme	properties	878:887	arg1	terms					815:819	terms	815:819	terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM	815:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	11	77	theme	structural	1900:1909	arg1	changes					1911:1917	ECM structural changes	1896:1917	ECM structural changes	1896:1917	Chemical changes are also accompanied by ECM structural changes and stiffening.
35673929	13	78	theme	high	2202:2205	arg1	severity					2210:2217	high OA severity	2202:2217	high OA severity	2202:2217	This yields damage strictly analogous to that observed at high OA severity.
35673929	4	79	from	nature	762:767	arg1	tissues					803:809	human cartilage tissues	787:809	human cartilage tissues	787:809	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	7	80	from	existence	1385:1393	arg1	progress					1419:1426	the OA progress	1412:1426	the OA progress	1412:1426	Taken together, these nanoscale characterization studies reveal the existence of two stages in the OA progress.
35673929	12	81	theme	imbalanced	1989:1998	arg1	activities					2000:2009	the imbalanced activities	1985:2009	the imbalanced activities of chondrocytes	1985:2025	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	0	82	theme	supramolecular	106:119	arg1	level					121:125	the supramolecular level	102:125	the supramolecular level	102:125	Deciphering pathological remodelling of the human cartilage extracellular matrix in osteoarthritis at the supramolecular level.
35673929	4	83	from	role	753:756	arg1	tissues					803:809	human cartilage tissues	787:809	human cartilage tissues	787:809	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	4	84	theme	assemblies	920:929	arg1	level					896:900	the level	892:900	the level of supramolecular assemblies constituting the cartilage ECM	892:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	14	85	theme	mechanistic	2241:2251	arg1	insights					2253:2260	mechanistic insights	2241:2260	mechanistic insights leading to a better understanding of the mechanism by which OA is initiated and progresses in the cartilage ECM	2241:2372	These findings bring mechanistic insights leading to a better understanding of the mechanism by which OA is initiated and progresses in the cartilage ECM.
35673929	4	86	from	terms	815:819	arg1	level					896:900	the level	892:900	the level of supramolecular assemblies constituting the cartilage ECM	892:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	9	87	theme	significant	1701:1711	arg1	degradation					1713:1723	significant degradation	1701:1723	significant degradation of the supramolecular assemblies	1701:1756	At the late stage, we observe a failed attempt to stabilize and/or restore the tissue, yielding significant degradation of the supramolecular assemblies.
35673929	0	88	theme	pathological	12:23	arg1	remodelling					25:35	pathological remodelling	12:35	pathological remodelling of the human cartilage extracellular matrix in osteoarthritis	12:97	Deciphering pathological remodelling of the human cartilage extracellular matrix in osteoarthritis at the supramolecular level.
35673929	15	89	theme	homeostatic	2463:2473	arg1	balance					2475:2481	the homeostatic balance	2459:2481	the homeostatic balance of chondrocyte metabolism	2459:2507	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	2	90	theme	changes	456:462	arg1	combination					417:427	a combination	415:427	a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease	415:537	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	12	91	theme	hyaluronidase	2113:2125	arg1	action					2103:2108	the combined action	2090:2108	the combined action of hyaluronidase and collagenase	2090:2141	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	9	92	theme	assemblies	1747:1756	arg1	degradation					1713:1723	significant degradation	1701:1723	significant degradation of the supramolecular assemblies	1701:1756	At the late stage, we observe a failed attempt to stabilize and/or restore the tissue, yielding significant degradation of the supramolecular assemblies.
35673929	10	93	theme	chondrocyte	1793:1803	arg1	activity					1805:1812	the chondrocyte activity	1789:1812	the chondrocyte activity	1789:1812	This suggests an imbalance in the chondrocyte activity that turns in favor of catabolic events.
35673929	0	94	theme	human	44:48	arg1	matrix					74:79	the human cartilage extracellular matrix	40:79	the human cartilage extracellular matrix	40:79	Deciphering pathological remodelling of the human cartilage extracellular matrix in osteoarthritis at the supramolecular level.
35673929	5	95	theme	X-ray	989:993	arg1	XPS					1023:1025	XPS	1023:1025	XPS	1023:1025	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	5	95	theme	X-ray	989:993	arg1	spectroscopy					1009:1020	X-ray photoelectron spectroscopy	989:1020	X-ray photoelectron spectroscopy (XPS)	989:1026	For this purpose, we used X-ray photoelectron spectroscopy (XPS), and developed an innovative methodological approach that provides the molecular composition of the ECM.
35673929	2	96	theme	pivotal	476:482	arg1	role					484:487	a pivotal role	474:487	a pivotal role	474:487	During the development of osteoarthritis (OA), the most common musculoskeletal disorder, the ECM is subjected to a combination of chemical and structural changes that play a pivotal role in the initiation and the progress of the disease.
35673929	11	97	theme	Chemical	1855:1862	arg1	changes					1864:1870	Chemical changes	1855:1870	Chemical changes	1855:1870	Chemical changes are also accompanied by ECM structural changes and stiffening.
35673929	0	98	theme	extracellular	60:72	arg1	matrix					74:79	the human cartilage extracellular matrix	40:79	the human cartilage extracellular matrix	40:79	Deciphering pathological remodelling of the human cartilage extracellular matrix in osteoarthritis at the supramolecular level.
35673929	10	99	from	imbalance	1776:1784	arg1	activity					1805:1812	the chondrocyte activity	1789:1812	the chondrocyte activity	1789:1812	This suggests an imbalance in the chondrocyte activity that turns in favor of catabolic events.
35673929	4	100	theme	cartilage	793:801	arg1	tissues					803:809	human cartilage tissues	787:809	human cartilage tissues	787:809	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	4	101	from	level	896:900	arg1	terms					815:819	terms	815:819	terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM	815:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	4	101	from	level	896:900	arg1	composition					833:843	chemical composition	824:843	chemical composition	824:843	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	4	101	from	level	896:900	arg1	properties					878:887	morphological and mechanical properties	849:887	morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM	849:960	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35673929	8	102	theme	cartilage	1590:1598	arg1	ECM					1600:1602	the cartilage ECM	1586:1602	the cartilage ECM	1586:1602	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	13	103	theme	analogous	2172:2180	arg1	damage					2156:2161	damage	2156:2161	damage strictly analogous to that observed at high OA severity	2156:2217	This yields damage strictly analogous to that observed at high OA severity.
35673929	11	104	theme	ECM	1896:1898	arg1	changes					1911:1917	ECM structural changes	1896:1917	ECM structural changes	1896:1917	Chemical changes are also accompanied by ECM structural changes and stiffening.
35673929	8	105	theme	homeostatic	1532:1542	arg1	activity					1556:1563	the homeostatic chondrocyte activity	1528:1563	the homeostatic chondrocyte activity that tends to repair the cartilage ECM	1528:1602	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	12	106	theme	combined	2094:2101	arg1	action					2103:2108	the combined action	2090:2108	the combined action of hyaluronidase and collagenase	2090:2141	Interestingly, we showed the possibility to mimic the imbalanced activities of chondrocytes by applying enzymatic digestions of healthy cartilage, through the combined action of hyaluronidase and collagenase.
35673929	15	107	theme	enzymatic	2532:2540	arg1	reactions					2542:2550	enzymatic reactions	2532:2550	enzymatic reactions involved in catabolic processes	2532:2582	They offer guidelines for the development of curative treatments, such as targeting the homeostatic balance of chondrocyte metabolism through the control of enzymatic reactions involved in catabolic processes.
35673929	1	108	theme	collagen	245:252	arg1	fibrils					254:260	entangled collagen fibrils	235:260	entangled collagen fibrils	235:260	The extracellular matrix (ECM) of articular cartilage is a three-dimensional network mainly constituted of entangled collagen fibrils and interfibrillar aggrecan aggregates.
35673929	8	109	located	observed	1507:1514	arg2	increase					1458:1465	a marked increase	1449:1465	a marked increase in the aggrecan and collagen content	1449:1502	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	8	109	located	observed	1507:1514	arg1	stage					1442:1446	the early stage	1432:1446	the early stage	1432:1446	At the early stage, a marked increase in the aggrecan and collagen content is observed, reflecting the homeostatic chondrocyte activity that tends to repair the cartilage ECM.
35673929	4	110	theme	OA	772:773	arg1	progress					775:782	OA progress	772:782	OA progress	772:782	Herein, we report a relevant way for unravelling the role and nature of OA progress on human cartilage tissues, in terms of chemical composition and morphological and mechanical properties at the level of supramolecular assemblies constituting the cartilage ECM.
35590486	10	0	theme	glycemic	1769:1776	arg1	index					1778:1782	glycemic index	1769:1782	glycemic index	1769:1782	This opens the alternative to be used in foods for modulating glycemic index.
35590486	9	1	from	nodosum	1657:1663	arg1	extracts					1631:1638	ultrasound-assisted extracts	1611:1638	ultrasound-assisted extracts from Ascophyllum nodosum	1611:1663	PRACTICAL APPLICATION: The study confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes.
35590486	2	2	theme	resin	399:403	arg1	purification					405:416	resin purification	399:416	resin purification	399:416	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	4	3	theme	inhibitory	860:869	arg1	activities					871:880	inhibitory activities	860:880	inhibitory activities of extracts	860:892	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	4	theme	antioxidant	643:653	arg1	capacity					655:662	antioxidant capacity	643:662	antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP)	643:719	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	9	5	theme	PRACTICAL	1564:1572	arg1	APPLICATION					1574:1584	PRACTICAL APPLICATION	1564:1584	PRACTICAL APPLICATION: The study confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes.	1564:1705	PRACTICAL APPLICATION: The study confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes.
35590486	1	6	theme	antidiabetic	173:184	arg1	characteristics					186:200	their antidiabetic characteristics	167:200	their antidiabetic characteristics	167:200	Seaweeds are gaining importance due to their antidiabetic characteristics.
35590486	5	7	theme	digestive	1082:1090	arg1	enzymes					1092:1098	both starch digestive enzymes	1070:1098	both starch digestive enzymes	1070:1098	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	5	8	dep	composition	943:953	arg1	the					930:932	the	930:932	the	930:932	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	2	9	theme	different	339:347	arg1	powers					360:365	different sonication powers	339:365	different sonication powers (70-90 W/cm2 )	339:380	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	2	9	theme	different	339:347	arg1	W/cm2					374:378	70-90 W/cm2	368:378	70-90 W/cm2	368:378	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	4	10	theme	characteristics	738:752	arg1	terms					614:618	terms	614:618	terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR	614:829	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	8	11	with	extracts	1502:1509	arg1	capacities					1541:1550	the different inhibitory capacities	1516:1550	the different inhibitory capacities of samples	1516:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	8	12	theme	quinones	1486:1493	arg1	presence					1435:1442	the presence	1431:1442	the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples	1431:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	7	13	theme	substrate	1365:1373	arg1	enzyme					1343:1348	the enzyme	1339:1348	the enzyme instead of the substrate	1339:1373	Seaweed extracts showed greater inhibition effect when they were preincubated with the enzyme instead of the substrate.
35590486	5	14	theme	increasing	997:1006	arg1	power					1019:1023	increasing sonication power	997:1023	increasing sonication power	997:1023	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	4	15	theme	capacity	655:662	arg1	characteristics					738:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics	623:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC]	623:817	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	15	theme	capacity	655:662	arg1	[RP-HPLC					809:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	16	theme	chromatography	794:807	arg1	characteristics					738:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics	623:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC]	623:817	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	16	theme	chromatography	794:807	arg1	[RP-HPLC					809:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	9	17	theme	ultrasound-assisted	1611:1629	arg1	extracts					1631:1638	ultrasound-assisted extracts	1611:1638	ultrasound-assisted extracts from Ascophyllum nodosum	1611:1663	PRACTICAL APPLICATION: The study confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes.
35590486	5	18	theme	starch	1075:1080	arg1	enzymes					1092:1098	both starch digestive enzymes	1070:1098	both starch digestive enzymes	1070:1098	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	4	19	theme	reversed-phase	755:768	arg1	chromatography					794:807	reversed-phase high-performance liquid chromatography	755:807	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	2	20	theme	α-glucosidase	441:453	arg1	enzymes					455:461	α-amylase and α-glucosidase enzymes	427:461	α-amylase and α-glucosidase enzymes	427:461	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	5	21	theme	Sonication	895:904	arg1	power					906:910	Sonication power	895:910	Sonication power	895:910	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	5	22	theme	sonication	1008:1017	arg1	power					1019:1023	increasing sonication power	997:1023	increasing sonication power	997:1023	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	3	23	theme	Different	464:472	arg1	methodologies					485:497	Different inhibition methodologies	464:497	Different inhibition methodologies	464:497	Different inhibition methodologies were carried out, preincubating the extract either with the enzyme or the substrate.
35590486	2	24	theme	ultrasound-assisted	303:321	arg1	extraction					323:332	ultrasound-assisted extraction	303:332	ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 )	303:380	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	6	25	theme	α-glucosidase	1206:1218	arg1	IC50					1184:1187	the IC50	1180:1187	the IC50 of α-amylase and α-glucosidase	1180:1218	The extract purification largely improved the inhibition efficiency decreasing the IC50 of α-amylase and α-glucosidase by 3.0 and 6.1 times, respectively.
35590486	2	26	dep	extracts	281:288	arg1	subjected					386:394	subjected	386:394	subjected to resin purification	386:416	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	2	26	dep	extracts	281:288	arg1	obtained					291:298	obtained	291:298	obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 )	291:380	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	2	27	theme	α-amylase	427:435	arg1	enzymes					455:461	α-amylase and α-glucosidase enzymes	427:461	α-amylase and α-glucosidase enzymes	427:461	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	0	28	theme	In	0:1	arg1	inhibition					9:18	In vitro inhibition	0:18	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum	0:116	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	5	29	theme	antiradical	959:969	arg1	activity					971:978	antiradical activity	959:978	antiradical activity	959:978	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	6	30	theme	extract	1105:1111	arg1	purification					1113:1124	The extract purification	1101:1124	The extract purification	1101:1124	The extract purification largely improved the inhibition efficiency decreasing the IC50 of α-amylase and α-glucosidase by 3.0 and 6.1 times, respectively.
35590486	5	31	theme	extracts	983:990	arg1	composition					943:953	proximal composition	934:953	proximal composition	934:953	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	7	32	theme	greater	1280:1286	arg1	effect					1299:1304	greater inhibition effect	1280:1304	greater inhibition effect	1280:1304	Seaweed extracts showed greater inhibition effect when they were preincubated with the enzyme instead of the substrate.
35590486	6	33	theme	α-amylase	1192:1200	arg1	IC50					1184:1187	the IC50	1180:1187	the IC50 of α-amylase and α-glucosidase	1180:1218	The extract purification largely improved the inhibition efficiency decreasing the IC50 of α-amylase and α-glucosidase by 3.0 and 6.1 times, respectively.
35590486	8	34	from	presence	1435:1442	arg1	extracts					1502:1509	the extracts	1498:1509	the extracts with the different inhibitory capacities of samples	1498:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	8	35	theme	uronic	1447:1452	arg1	complexes					1472:1480	uronic acids-polyphenols complexes	1447:1480	uronic acids-polyphenols complexes	1447:1480	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	8	36	theme	inhibitory	1530:1539	arg1	capacities					1541:1550	the different inhibitory capacities	1516:1550	the different inhibitory capacities of samples	1516:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	7	37	theme	inhibition	1288:1297	arg1	effect					1299:1304	greater inhibition effect	1280:1304	greater inhibition effect	1280:1304	Seaweed extracts showed greater inhibition effect when they were preincubated with the enzyme instead of the substrate.
35590486	4	38	theme	analysis	633:640	arg1	characteristics					738:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics	623:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC]	623:817	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	38	theme	analysis	633:640	arg1	[RP-HPLC					809:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	2	39	theme	sonication	349:358	arg1	powers					360:365	different sonication powers	339:365	different sonication powers (70-90 W/cm2 )	339:380	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	2	39	theme	sonication	349:358	arg1	W/cm2					374:378	70-90 W/cm2	368:378	70-90 W/cm2	368:378	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	9	40	theme	digestive	1688:1696	arg1	enzymes					1698:1704	digestive enzymes	1688:1704	digestive enzymes	1688:1704	PRACTICAL APPLICATION: The study confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes.
35590486	5	41	theme	proximal	934:941	arg1	composition					943:953	proximal composition	934:953	proximal composition	934:953	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	0	42	theme	digestive	30:38	arg1	enzymes					40:46	starch digestive enzymes	23:46	starch digestive enzymes	23:46	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	4	43	theme	proximate	623:631	arg1	analysis					633:640	proximate analysis	623:640	proximate analysis	623:640	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	44	dep	capacity	655:662	arg1	[DPPH					704:708	2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH]	665:709	2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH]	665:709	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	44	dep	capacity	655:662	arg1	FRAP					715:718	FRAP	715:718	FRAP	715:718	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	2	45	theme	nodosum	273:279	arg1	extracts					281:288	aqueous Ascophyllum nodosum extracts	253:288	aqueous Ascophyllum nodosum extracts	253:288	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	9	46	used	used	1672:1675	arg2	extracts					1631:1638	ultrasound-assisted extracts	1611:1638	ultrasound-assisted extracts from Ascophyllum nodosum	1611:1663	PRACTICAL APPLICATION: The study confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes.
35590486	8	47	theme	H-NMR	1400:1404	arg1	spectra					1406:1412	1 H-NMR spectra	1398:1412	1 H-NMR spectra	1398:1412	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	0	48	theme	starch	23:28	arg1	enzymes					40:46	starch digestive enzymes	23:46	starch digestive enzymes	23:46	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	3	49	theme	inhibition	474:483	arg1	methodologies					485:497	Different inhibition methodologies	464:497	Different inhibition methodologies	464:497	Different inhibition methodologies were carried out, preincubating the extract either with the enzyme or the substrate.
35590486	2	50	theme	Ascophyllum	261:271	arg1	extracts					281:288	aqueous Ascophyllum nodosum extracts	253:288	aqueous Ascophyllum nodosum extracts	253:288	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	6	51	theme	inhibition	1147:1156	arg1	efficiency					1158:1167	the inhibition efficiency	1143:1167	the inhibition efficiency	1143:1167	The extract purification largely improved the inhibition efficiency decreasing the IC50 of α-amylase and α-glucosidase by 3.0 and 6.1 times, respectively.
35590486	4	52	from	characterization	593:608	arg1	terms					614:618	terms	614:618	terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR	614:829	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	53	theme	Chemical	584:591	arg1	characterization					593:608	Chemical characterization	584:608	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR)	584:830	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	8	54	attach	presence	1435:1442	arg1	extracts					1502:1509	the extracts	1498:1509	the extracts with the different inhibitory capacities of samples	1498:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	8	54	attach	presence	1435:1442	arg2	complexes					1472:1480	uronic acids-polyphenols complexes	1447:1480	uronic acids-polyphenols complexes	1447:1480	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	8	54	attach	presence	1435:1442	arg2	quinones					1486:1493	quinones	1486:1493	quinones	1486:1493	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	2	55	theme	aqueous	253:259	arg1	extracts					281:288	aqueous Ascophyllum nodosum extracts	253:288	aqueous Ascophyllum nodosum extracts	253:288	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	0	56	theme	enzymes	40:46	arg1	inhibition					9:18	In vitro inhibition	0:18	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum	0:116	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	9	57	dep	APPLICATION	1574:1584	arg1	confirms					1597:1604	confirms	1597:1604	confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes	1597:1704	PRACTICAL APPLICATION: The study confirms that ultrasound-assisted extracts from Ascophyllum nodosum can be used to inhibit digestive enzymes.
35590486	5	58	theme	inhibition	1035:1044	arg1	%					1059:1059	>15%	1056:1059	>15%	1056:1059	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	5	58	theme	inhibition	1035:1044	arg1	capacity 					1046:1054	inhibition capacity 	1035:1054	inhibition capacity (>15%) against both starch digestive enzymes	1035:1098	Sonication power did not influence the proximal composition nor antiradical activity of extracts, but increasing sonication power increased inhibition capacity (>15%) against both starch digestive enzymes.
35590486	2	59	theme	extracts	281:288	arg1	effects					242:248	the inhibitory effects	227:248	the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes	227:461	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	7	60	theme	Seaweed	1256:1262	arg1	extracts					1264:1271	Seaweed extracts	1256:1271	Seaweed extracts	1256:1271	Seaweed extracts showed greater inhibition effect when they were preincubated with the enzyme instead of the substrate.
35590486	2	61	theme	inhibitory	231:240	arg1	effects					242:248	the inhibitory effects	227:248	the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes	227:461	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	4	62	theme	extracts	885:892	arg1	activities					871:880	inhibitory activities	860:880	inhibitory activities of extracts	860:892	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	8	63	theme	different	1520:1528	arg1	capacities					1541:1550	the different inhibitory capacities	1516:1550	the different inhibitory capacities of samples	1516:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	4	64	theme	H-NMR	825:829	arg1	terms					614:618	terms	614:618	terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR	614:829	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	2	65	with	extraction	323:332	arg1	powers					360:365	different sonication powers	339:365	different sonication powers (70-90 W/cm2 )	339:380	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	2	65	with	extraction	323:332	arg1	W/cm2					374:378	70-90 W/cm2	368:378	70-90 W/cm2	368:378	This study investigated the inhibitory effects of aqueous Ascophyllum nodosum extracts, obtained by ultrasound-assisted extraction with different sonication powers (70-90 W/cm2 ) and subjected to resin purification, against α-amylase and α-glucosidase enzymes.
35590486	3	66	dep	enzyme	559:564	arg1	either					543:548	either	543:548	either	543:548	Different inhibition methodologies were carried out, preincubating the extract either with the enzyme or the substrate.
35590486	0	67	from	nodosum	110:116	arg1	polyphenols					81:91	ultrasound-assisted extracted polyphenols	51:91	ultrasound-assisted extracted polyphenols from Ascophyllum nodosum	51:116	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	8	68	theme	samples	1555:1561	arg1	capacities					1541:1550	the different inhibitory capacities	1516:1550	the different inhibitory capacities of samples	1516:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	8	69	theme	acids-polyphenols	1454:1470	arg1	complexes					1472:1480	uronic acids-polyphenols complexes	1447:1480	uronic acids-polyphenols complexes	1447:1480	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	4	70	theme	2,2-diphenyl-1-picryl-hydrazyl-hydrate	665:702	arg1	[DPPH					704:708	2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH]	665:709	2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH]	665:709	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	0	71	theme	extracted	71:79	arg1	polyphenols					81:91	ultrasound-assisted extracted polyphenols	51:91	ultrasound-assisted extracted polyphenols from Ascophyllum nodosum	51:116	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	8	72	theme	complexes	1472:1480	arg1	presence					1435:1442	the presence	1431:1442	the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples	1431:1561	RP-HPLC together with 1 H-NMR spectra allowed relating the presence of uronic acids-polyphenols complexes and quinones in the extracts with the different inhibitory capacities of samples.
35590486	4	73	theme	liquid	787:792	arg1	chromatography					794:807	reversed-phase high-performance liquid chromatography	755:807	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	74	theme	polyphenols	726:736	arg1	characteristics					738:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics	623:752	proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC]	623:817	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	4	74	theme	polyphenols	726:736	arg1	[RP-HPLC					809:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
35590486	0	75	theme	ultrasound-assisted	51:69	arg1	polyphenols					81:91	ultrasound-assisted extracted polyphenols	51:91	ultrasound-assisted extracted polyphenols from Ascophyllum nodosum	51:116	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	0	76	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro inhibition of starch digestive enzymes by ultrasound-assisted extracted polyphenols from Ascophyllum nodosum seaweeds.
35590486	4	77	theme	high-performance	770:785	arg1	chromatography					794:807	reversed-phase high-performance liquid chromatography	755:807	reversed-phase high-performance liquid chromatography [RP-HPLC	755:816	Chemical characterization, in terms of proximate analysis, antioxidant capacity (2,2-diphenyl-1-picryl-hydrazyl-hydrate [DPPH] and FRAP), and polyphenols characteristics (reversed-phase high-performance liquid chromatography [RP-HPLC] and 1 H-NMR) were carried out to explain inhibitory activities of extracts.
36126853	6	0	theme	BCAA	851:854	arg1	diet					856:859	a high BCAA diet	844:859	a high BCAA diet	844:859	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	9	1	theme	gut	1548:1550	arg1	microbiota					1552:1561	gut microbiota	1548:1561	gut microbiota	1548:1561	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	2	theme	protein	1159:1165	arg1	concentrations					1167:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	3	3	theme	dietary	477:483	arg1	supplement					485:494	a common dietary supplement	468:494	a common dietary supplement	468:494	The branched-chain amino acids (BCAAs), as a common dietary supplement, could modulate brain function.
36126853	1	4	theme	gut	163:165	arg1	microbiota					167:176	gut microbiota	163:176	gut microbiota	163:176	Neuroinflammation caused by the disorder of gut microbiota and its metabolites is associated with the pathogenesis of Parkinson's disease (PD).
36126853	6	5	theme	rotenone-induced	811:826	arg1	mice					828:831	rotenone-induced mice	811:831	rotenone-induced mice	811:831	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	9	6	theme	metabolism	1572:1581	arg1	changes					1537:1543	the dynamic changes	1525:1543	the dynamic changes of gut microbiota and BCAA metabolism	1525:1581	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	6	7	theme	high	846:849	arg1	diet					856:859	a high BCAA diet	844:859	a high BCAA diet	844:859	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	4	8	theme	microbial	579:587	arg1	community					589:597	microbial community	579:597	microbial community	579:597	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	0	9	theme	animal	105:110	arg1	study					112:116	An animal study	102:116	An animal study	102:116	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	1	10	theme	metabolites	186:196	arg1	disorder					151:158	the disorder	147:158	the disorder of gut microbiota and its metabolites	147:196	Neuroinflammation caused by the disorder of gut microbiota and its metabolites is associated with the pathogenesis of Parkinson's disease (PD).
36126853	9	11	theme	novel	1597:1601	arg1	strategy					1603:1610	a novel strategy	1595:1610	a novel strategy for PD therapy	1595:1625	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	12	theme	pro-inflammatory	1200:1215	arg1	factor-α					1251:1258	tumor necrosis factor-α	1236:1258	tumor necrosis factor-α	1236:1258	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	12	theme	pro-inflammatory	1200:1215	arg1	factors					1217:1223	pro-inflammatory factors	1200:1223	pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6)	1200:1290	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	12	theme	pro-inflammatory	1200:1215	arg1	[IL					1273:1275	interleukin [IL]-1β	1261:1279	interleukin [IL]-1β	1261:1279	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	12	theme	pro-inflammatory	1200:1215	arg1	IL-6					1286:1289	IL-6	1286:1289	IL-6	1286:1289	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	13	from	levels	1393:1398	arg1	mice					1406:1409	PD mice	1403:1409	PD mice	1403:1409	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	5	14	theme	chromatography-mass	771:789	arg1	spectrometry					791:802	gas chromatography-mass spectrometry	767:802	gas chromatography-mass spectrometry	767:802	Serum BCAAs were determined by gas chromatography-mass spectrometry.
36126853	8	15	theme	lipopolysaccharide	1132:1149	arg1	concentrations					1167:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	16	theme	high	1345:1348	arg1	diet					1355:1358	the high BCAA diet	1341:1358	the high BCAA diet	1341:1358	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	7	17	theme	mouse	970:974	arg1	model					976:980	a PD mouse model	965:980	a PD mouse model	965:980	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	8	18	theme	tumor	1236:1240	arg1	factor-α					1251:1258	tumor necrosis factor-α	1236:1258	tumor necrosis factor-α	1236:1258	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	19	from	levels	1190:1195	arg1	colon					1299:1303	colon	1299:1303	colon	1299:1303	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	19	from	levels	1190:1195	arg1	nigra					1320:1324	substantia nigra	1309:1324	substantia nigra	1309:1324	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	9	20	theme	BCAA	1635:1638	arg1	intervention					1645:1656	a high BCAA diet intervention	1628:1656	a high BCAA diet intervention	1628:1656	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	21	theme	inflammatory	1380:1391	arg1	levels					1393:1398	the inflammatory levels	1376:1398	the inflammatory levels in PD mice	1376:1409	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	22	theme	interleukin	1261:1271	arg1	[IL					1273:1275	interleukin [IL]-1β	1261:1279	interleukin [IL]-1β	1261:1279	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	0	23	from	Role	0:3	arg1	pathogenesis					65:76	the pathogenesis	61:76	the pathogenesis of Parkinson's disease	61:99	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	6	24	theme	dopaminergic	908:919	arg1	loss					928:931	dopaminergic neuron loss	908:931	dopaminergic neuron loss	908:931	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	3	25	theme	branched-chain	429:442	arg1	BCAAs					457:461	BCAAs	457:461	BCAAs	457:461	The branched-chain amino acids (BCAAs), as a common dietary supplement, could modulate brain function.
36126853	3	25	theme	branched-chain	429:442	arg1	acids					450:454	The branched-chain amino acids	425:454	The branched-chain amino acids (BCAAs)	425:462	The branched-chain amino acids (BCAAs), as a common dietary supplement, could modulate brain function.
36126853	7	26	theme	BCAAs	1106:1110	arg1	decrease					1094:1101	the peripheral decrease	1079:1101	the peripheral decrease of BCAAs	1079:1110	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	0	27	theme	amino	46:50	arg1	acids					52:56	gut microbiota-derived branched-chain amino acids	8:56	gut microbiota-derived branched-chain amino acids	8:56	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	4	28	theme	gene	662:665	arg1	sequencing					667:676	rRNA gene sequencing	657:676	rRNA gene sequencing	657:676	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	5	29	theme	Serum	736:740	arg1	BCAAs					742:746	Serum BCAAs	736:746	Serum BCAAs	736:746	Serum BCAAs were determined by gas chromatography-mass spectrometry.
36126853	8	30	theme	motor	1424:1428	arg1	dysfunctions					1444:1455	motor and non-motor dysfunctions	1424:1455	dysfunctions	1444:1455	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	0	31	theme	gut	8:10	arg1	acids					52:56	gut microbiota-derived branched-chain amino acids	8:56	gut microbiota-derived branched-chain amino acids	8:56	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	8	32	theme	non-motor	1434:1442	arg1	dysfunctions					1444:1455	motor and non-motor dysfunctions	1424:1455	dysfunctions	1444:1455	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	0	33	theme	branched-chain	31:44	arg1	acids					52:56	gut microbiota-derived branched-chain amino acids	8:56	gut microbiota-derived branched-chain amino acids	8:56	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	9	34	theme	inflammation	1715:1726	arg1	levels					1705:1710	the levels	1701:1710	the levels of inflammation	1701:1726	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	35	theme	substantia	1309:1318	arg1	nigra					1320:1324	substantia nigra	1309:1324	substantia nigra	1309:1324	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	0	36	theme	acids	52:56	arg1	Role					0:3	Role	0:3	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease	0:99	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	8	37	theme	neuron	1474:1479	arg1	impairment					1481:1490	dopaminergic neuron impairment	1461:1490	dopaminergic neuron impairment	1461:1490	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	4	38	theme	different	715:723	arg1	stages					728:733	different PD stages	715:733	different PD stages	715:733	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	3	39	theme	amino	444:448	arg1	BCAAs					457:461	BCAAs	457:461	BCAAs	457:461	The branched-chain amino acids (BCAAs), as a common dietary supplement, could modulate brain function.
36126853	3	39	theme	amino	444:448	arg1	acids					450:454	The branched-chain amino acids	425:454	The branched-chain amino acids (BCAAs)	425:462	The branched-chain amino acids (BCAAs), as a common dietary supplement, could modulate brain function.
36126853	9	40	theme	dynamic	1529:1535	arg1	changes					1537:1543	the dynamic changes	1525:1543	the dynamic changes of gut microbiota and BCAA metabolism	1525:1581	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	4	41	theme	longitudinal	556:567	arg1	shifts					569:574	the longitudinal shifts	552:574	the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks	552:648	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	2	42	attach	derived	315:321	arg2	molecules					305:313	certain molecules	297:313	certain molecules derived from gut microbiota	297:341	Thus, it is necessary to identify certain molecules derived from gut microbiota to verify whether they could become intervention targets for the treatment of PD.
36126853	2	42	attach	derived	315:321	arg1	microbiota					332:341	gut microbiota	328:341	gut microbiota	328:341	Thus, it is necessary to identify certain molecules derived from gut microbiota to verify whether they could become intervention targets for the treatment of PD.
36126853	0	43	link	microbiota-derived	12:29	arg1	acids					52:56	gut microbiota-derived branched-chain amino acids	8:56	gut microbiota-derived branched-chain amino acids	8:56	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	1	44	theme	disease	249:255	arg1	pathogenesis					221:232	the pathogenesis	217:232	the pathogenesis of Parkinson's disease (PD)	217:260	Neuroinflammation caused by the disorder of gut microbiota and its metabolites is associated with the pathogenesis of Parkinson's disease (PD).
36126853	9	45	theme	microbiota	1552:1561	arg1	changes					1537:1543	the dynamic changes	1525:1543	the dynamic changes of gut microbiota and BCAA metabolism	1525:1581	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	46	dep	colon	1299:1303	arg1	the					1295:1297	the	1295:1297	the	1295:1297	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	7	47	theme	PD	1009:1010	arg1	progression					1012:1022	PD progression	1009:1022	PD progression	1009:1022	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	3	48	theme	common	470:475	arg1	supplement					485:494	a common dietary supplement	468:494	a common dietary supplement	468:494	The branched-chain amino acids (BCAAs), as a common dietary supplement, could modulate brain function.
36126853	7	49	theme	microbiota	1048:1057	arg1	compositions					1059:1070	gut microbiota compositions	1044:1070	gut microbiota compositions	1044:1070	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	9	50	theme	BCAA	1567:1570	arg1	metabolism					1572:1581	BCAA metabolism	1567:1581	BCAA metabolism	1567:1581	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	51	theme	binding	1151:1157	arg1	concentrations					1167:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	3	52	theme	brain	512:516	arg1	function					518:525	brain function	512:525	brain function	512:525	The branched-chain amino acids (BCAAs), as a common dietary supplement, could modulate brain function.
36126853	1	53	theme	microbiota	167:176	arg1	disorder					151:158	the disorder	147:158	the disorder of gut microbiota and its metabolites	147:196	Neuroinflammation caused by the disorder of gut microbiota and its metabolites is associated with the pathogenesis of Parkinson's disease (PD).
36126853	6	54	theme	non-motor	887:895	arg1	functions					897:905	the motor and non-motor functions	873:905	functions	897:905	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	5	55	theme	gas	767:769	arg1	spectrometry					791:802	gas chromatography-mass spectrometry	767:802	gas chromatography-mass spectrometry	767:802	Serum BCAAs were determined by gas chromatography-mass spectrometry.
36126853	6	56	theme	motor	877:881	arg1	functions					897:905	the motor and non-motor functions	873:905	functions	897:905	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	8	57	theme	BCAA	1350:1353	arg1	diet					1355:1358	the high BCAA diet	1341:1358	the high BCAA diet	1341:1358	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	4	58	theme	community	589:597	arg1	shifts					569:574	the longitudinal shifts	552:574	the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks	552:648	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	7	59	theme	PD	967:968	arg1	model					976:980	a PD mouse model	965:980	a PD mouse model	965:980	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	8	60	from	concentrations	1167:1180	arg1	colon					1299:1303	colon	1299:1303	colon	1299:1303	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	60	from	concentrations	1167:1180	arg1	nigra					1320:1324	substantia nigra	1309:1324	substantia nigra	1309:1324	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	61	theme	serum	1126:1130	arg1	concentrations					1167:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	9	62	theme	PD	1616:1617	arg1	therapy					1619:1625	PD therapy	1616:1625	PD therapy	1616:1625	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	63	theme	factors	1217:1223	arg1	concentrations					1167:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	the serum lipopolysaccharide binding protein concentrations	1122:1180	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	8	63	theme	factors	1217:1223	arg1	levels					1190:1195	the levels	1186:1195	the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra	1186:1324	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	4	64	from	shifts	569:574	arg1	mice					602:605	mice	602:605	mice treated with rotenone for 0, 3 and 4 weeks	602:648	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	6	65	theme	inflammation	938:949	arg1	levels					951:956	inflammation levels	938:956	inflammation levels	938:956	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	9	66	theme	high	1630:1633	arg1	intervention					1645:1656	a high BCAA diet intervention	1628:1656	a high BCAA diet intervention	1628:1656	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	67	theme	necrosis	1242:1249	arg1	factor-α					1251:1258	tumor necrosis factor-α	1236:1258	tumor necrosis factor-α	1236:1258	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	0	68	dep	study	112:116	arg1	Role					0:3	Role	0:3	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease	0:99	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	9	69	theme	diet	1640:1643	arg1	intervention					1645:1656	a high BCAA diet intervention	1628:1656	a high BCAA diet intervention	1628:1656	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	8	70	theme	PD	1403:1404	arg1	mice					1406:1409	PD mice	1403:1409	PD mice	1403:1409	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	0	71	theme	disease	93:99	arg1	pathogenesis					65:76	the pathogenesis	61:76	the pathogenesis of Parkinson's disease	61:99	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	6	72	theme	neuron	921:926	arg1	loss					928:931	dopaminergic neuron loss	908:931	dopaminergic neuron loss	908:931	Then, rotenone-induced mice were given a high BCAA diet to evaluate the motor and non-motor functions, dopaminergic neuron loss, and inflammation levels.
36126853	9	73	theme	PD	1672:1673	arg1	progression					1675:1685	PD progression	1672:1685	PD progression	1672:1685	Together, our results emphasize the dynamic changes of gut microbiota and BCAA metabolism and propose a novel strategy for PD therapy: a high BCAA diet intervention could improve PD progression by regulating the levels of inflammation.
36126853	4	74	theme	microbial	694:702	arg1	markers					704:710	the microbial markers	690:710	the microbial markers	690:710	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	7	75	theme	peripheral	1083:1092	arg1	decrease					1094:1101	the peripheral decrease	1079:1101	the peripheral decrease of BCAAs	1079:1110	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	4	76	theme	rRNA	657:660	arg1	sequencing					667:676	rRNA gene sequencing	657:676	rRNA gene sequencing	657:676	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	0	77	theme	microbiota-derived	12:29	arg1	acids					52:56	gut microbiota-derived branched-chain amino acids	8:56	gut microbiota-derived branched-chain amino acids	8:56	Role of gut microbiota-derived branched-chain amino acids in the pathogenesis of Parkinson's disease: An animal study.
36126853	2	78	theme	intervention	379:390	arg1	targets					392:398	intervention targets	379:398	intervention targets for the treatment of PD	379:422	Thus, it is necessary to identify certain molecules derived from gut microbiota to verify whether they could become intervention targets for the treatment of PD.
36126853	7	79	theme	compositions	1059:1070	arg1	alterations					1029:1039	the alterations	1025:1039	the alterations of gut microbiota compositions	1025:1070	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	8	80	theme	dopaminergic	1461:1472	arg1	impairment					1481:1490	dopaminergic neuron impairment	1461:1490	dopaminergic neuron impairment	1461:1490	Based on the serum lipopolysaccharide binding protein concentrations and the levels of pro-inflammatory factors (including tumor necrosis factor-α, interleukin [IL]-1β, and IL-6) in the colon and substantia nigra, we found that the high BCAA diet could attenuate the inflammatory levels in PD mice, and reverse motor and non-motor dysfunctions and dopaminergic neuron impairment.
36126853	7	81	theme	gut	1044:1046	arg1	compositions					1059:1070	gut microbiota compositions	1044:1070	gut microbiota compositions	1044:1070	Using a PD mouse model, we discovered that during PD progression, the alterations of gut microbiota compositions led to the peripheral decrease of BCAAs.
36126853	2	82	theme	PD	421:422	arg1	treatment					408:416	the treatment	404:416	the treatment of PD	404:422	Thus, it is necessary to identify certain molecules derived from gut microbiota to verify whether they could become intervention targets for the treatment of PD.
36126853	2	83	theme	certain	297:303	arg1	molecules					305:313	certain molecules	297:313	certain molecules derived from gut microbiota	297:341	Thus, it is necessary to identify certain molecules derived from gut microbiota to verify whether they could become intervention targets for the treatment of PD.
36126853	4	84	theme	PD	725:726	arg1	stages					728:733	different PD stages	715:733	different PD stages	715:733	Herein, we investigated the longitudinal shifts of microbial community in mice treated with rotenone for 0, 3 and 4 weeks by 16S rRNA gene sequencing to identify the microbial markers at different PD stages.
36126853	2	85	theme	gut	328:330	arg1	microbiota					332:341	gut microbiota	328:341	gut microbiota	328:341	Thus, it is necessary to identify certain molecules derived from gut microbiota to verify whether they could become intervention targets for the treatment of PD.
37108827	7	0	theme	sampling	1412:1419	arg1	depth					1421:1425	a sampling depth	1410:1425	a sampling depth about 10 nm	1410:1437	Since antimicrobial activity is largely determined by the surface composition, much attention was paid to studying its properties by XPS, a surface-sensitive method, at a sampling depth about 10 nm.
37108827	9	1	theme	celluloses	1546:1555	arg1	spectra					1504:1510	XPS C 1s spectra	1495:1510	XPS C 1s spectra of the original and Ag-containing celluloses	1495:1555	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	1	2	theme	bacterial	249:257	arg1	BC					270:271	BC	270:271	BC	270:271	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	1	2	theme	bacterial	249:257	arg1	cellulose					259:267	bacterial cellulose	249:267	bacterial cellulose (BC)	249:272	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	11	3	theme	spherical	1900:1908	arg1	beads					1910:1914	spherical beads	1900:1914	spherical beads	1900:1914	Ag NPs in the BC films and spherical beads were mainly in the zerovalent state.
37108827	14	4	theme	medical	2356:2362	arg1	application					2364:2374	their medical application	2350:2374	their medical application	2350:2374	These results increase the possibility of their medical application.
37108827	5	5	with	interaction	770:780	arg1	substances					888:897	organic substances	880:897	organic substances during their co-condensation on the cooled walls of a reaction vessel	880:967	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	6	6	theme	X-ray	1201:1205	arg1	XPS					1235:1237	XPS	1235:1237	XPS	1235:1237	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	6	theme	X-ray	1201:1205	arg1	spectroscopy					1221:1232	X-ray photoelectron spectroscopy	1201:1232	X-ray photoelectron spectroscopy (XPS)	1201:1238	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	3	7	theme	spherical	513:521	arg1	beads					526:530	spherical BC beads	513:530	spherical BC beads (SBCB)	513:537	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	3	7	theme	spherical	513:521	arg1	SBCB					533:536	SBCB	533:536	SBCB	533:536	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	12	8	dep	Escherichia	2082:2092	arg1	coli					2094:2097	coli	2094:2097	coli	2094:2097	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
37108827	10	9	theme	silver	1794:1799	arg1	nanoparticles					1801:1813	silver nanoparticles	1794:1813	silver nanoparticles with a size of less than 3 nm in the near-surface region	1794:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	6	10	from	state	1013:1017	arg1	materials					1039:1047	the materials	1035:1047	the materials	1035:1047	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	5	11	theme	reactive	795:802	arg1	metals					811:816	extremely reactive atomic metals	785:816	extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa	785:873	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	1	12	theme	renewable	162:170	arg1	resources					172:180	renewable resources	162:180	renewable resources for the production of medical materials	162:220	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	12	13	theme	Ag	1982:1983	arg1	nanoparticles					1985:1997	Ag nanoparticles	1982:1997	Ag nanoparticles	1982:1997	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
37108827	0	14	theme	Biological	108:117	arg1	Forms					52:56	Various Forms	44:56	Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity	44:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	14	theme	Biological	108:117	arg1	Activity					119:126	Biological Activity	108:126	Biological Activity	108:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	3	15	theme	beads	526:530	arg1	form					489:492	the form	485:492	the form of films (BCF) and spherical BC beads (SBCB)	485:537	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	12	16	dep	subtilis	2049:2056	arg1	fungi					2151:2155	fungi	2151:2155	fungi	2151:2155	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
37108827	9	17	theme	groups	1608:1613	arg1	intensity					1583:1591	the intensity	1579:1591	the intensity of the C-C/C-H groups in the latter	1579:1627	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	7	18	theme	much	1320:1323	arg1	attention					1325:1333	much attention	1320:1333	much attention	1320:1333	Since antimicrobial activity is largely determined by the surface composition, much attention was paid to studying its properties by XPS, a surface-sensitive method, at a sampling depth about 10 nm.
37108827	3	19	theme	Gluconacetobacter	546:562	arg1	strain					583:588	the Gluconacetobacter hansenii GH-1/2008 strain	542:588	the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions	542:624	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	5	20	from	evaporation	828:838	arg1	vacuum					843:848	vacuum	843:848	vacuum	843:848	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	1	21	theme	medical	204:210	arg1	materials					212:220	medical materials	204:220	medical materials	204:220	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	9	22	from	increase	1567:1574	arg1	intensity					1583:1591	the intensity	1579:1591	the intensity of the C-C/C-H groups in the latter	1579:1627	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	6	23	theme	small-angle	1161:1171	arg1	SAXS					1191:1194	SAXS	1191:1194	SAXS	1191:1194	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	23	theme	small-angle	1161:1171	arg1	scattering					1179:1188	small-angle X-ray scattering	1161:1188	small-angle X-ray scattering (SAXS)	1161:1195	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	1	24	from	research	237:244	arg1	BC					270:271	BC	270:271	BC	270:271	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	1	24	from	research	237:244	arg1	nanocomposites					278:291	nanocomposites	278:291	nanocomposites	278:291	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	1	24	from	research	237:244	arg1	cellulose					259:267	bacterial cellulose	249:267	bacterial cellulose (BC)	249:272	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	4	25	theme	polymer	701:707	arg1	matrix					709:714	the polymer matrix	697:714	the polymer matrix using metal-containing organosol	697:747	The Ag nanoparticles synthesized in 2-propanol were incorporated into the polymer matrix using metal-containing organosol.
37108827	6	26	theme	X-ray	1136:1140	arg1	XRD					1155:1157	XRD	1155:1157	XRD	1155:1157	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	26	theme	X-ray	1136:1140	arg1	diffraction					1142:1152	powder X-ray diffraction	1129:1152	powder X-ray diffraction (XRD)	1129:1158	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	1	27	theme	Sustained	129:137	arg1	interest					139:146	Sustained interest	129:146	Sustained interest in the use of renewable resources for the production of medical materials	129:220	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	10	28	theme	near-surface	1852:1863	arg1	region					1865:1870	the near-surface region	1848:1870	the near-surface region	1848:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	0	29	theme	Bacterial	61:69	arg1	Cellulose					71:79	Bacterial Cellulose	61:79	Bacterial Cellulose	61:79	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	9	30	theme	Ag	1708:1709	arg1	nanoparticles					1693:1705	Ag nanoparticles	1690:1705	Ag nanoparticles (Ag NPs)	1690:1714	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	9	30	theme	Ag	1708:1709	arg1	NPs					1711:1713	Ag NPs	1708:1713	Ag NPs	1708:1713	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	5	31	theme	10-2	867:870	arg1	Pa					872:873	10-2 Pa	867:873	10-2 Pa	867:873	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	6	32	from	structure	987:995	arg1	materials					1039:1047	the materials	1035:1047	the materials	1035:1047	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	9	33	theme	XPS	1495:1497	arg1	spectra					1504:1510	XPS C 1s spectra	1495:1510	XPS C 1s spectra of the original and Ag-containing celluloses	1495:1555	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	1	34	from	interest	139:146	arg1	use					155:157	the use	151:157	the use of renewable resources for the production of medical materials	151:220	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	12	35	theme	Candida	2112:2118	arg1	albicans					2120:2127	Candida albicans	2112:2127	Candida albicans	2112:2127	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
37108827	5	36	theme	reaction	953:960	arg1	vessel					962:967	a reaction vessel	951:967	a reaction vessel	951:967	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	9	37	theme	Ag	1690:1691	arg1	nanoparticles					1693:1705	Ag nanoparticles	1690:1705	Ag nanoparticles (Ag NPs)	1690:1714	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	9	37	theme	Ag	1690:1691	arg1	NPs					1711:1713	Ag NPs	1708:1713	Ag NPs	1708:1713	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	6	38	from	composition	974:984	arg1	materials					1039:1047	the materials	1035:1047	the materials	1035:1047	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	0	39	theme	Hybrid	0:5	arg1	Materials					25:33	Hybrid Silver-Containing Materials	0:33	Hybrid Silver-Containing Materials	0:33	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	13	40	theme	Aspergillus	2283:2293	arg1	niger					2295:2299	Aspergillus niger	2283:2299	Aspergillus niger fungi	2283:2305	It was found that AgNPs/SBCB nanocomposites are more active than Ag NPs/BCF samples, especially against Candida albicans and Aspergillus niger fungi.
37108827	6	41	theme	metal	1026:1030	arg1	structure					987:995	structure	987:995	structure	987:995	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	41	theme	metal	1026:1030	arg1	composition					974:984	composition	974:984	composition	974:984	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	41	theme	metal	1026:1030	arg1	state					1013:1017	electronic state	1002:1017	electronic state	1002:1017	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	8	42	theme	O	1449:1449	arg1	spectra					1454:1460	O 1s spectra	1449:1460	O 1s spectra	1449:1460	C 1s and O 1s spectra were analyzed self-consistently.
37108827	12	43	theme	Escherichia	2082:2092	arg1	bacteria					2099:2106	Escherichia coli bacteria	2082:2106	Escherichia coli bacteria	2082:2106	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
37108827	3	44	theme	Bacterial	449:457	arg1	cellulose					459:467	Bacterial cellulose	449:467	Bacterial cellulose	449:467	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	5	45	theme	metals	811:816	arg1	interaction					770:780	the interaction	766:780	the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel	766:967	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	5	46	theme	cooled	935:940	arg1	walls					942:946	the cooled walls	931:946	the cooled walls of a reaction vessel	931:967	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	9	47	theme	1s	1501:1502	arg1	spectra					1504:1510	XPS C 1s spectra	1495:1510	XPS C 1s spectra of the original and Ag-containing celluloses	1495:1555	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	6	48	theme	electronic	1002:1011	arg1	state					1013:1017	electronic state	1002:1017	electronic state	1002:1017	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	10	49	theme	size	1721:1724	arg1	effect					1726:1731	The size effect	1717:1731	The size effect	1717:1731	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	6	50	theme	electron	1097:1104	arg1	microscopy					1106:1115	transmission and scanning electron microscopy	1071:1115	microscopy	1106:1115	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	3	51	theme	GH-1/2008	573:581	arg1	strain					583:588	the Gluconacetobacter hansenii GH-1/2008 strain	542:588	the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions	542:624	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	0	52	dep	Forms	52:56	arg1	Structure					93:101	Structure	93:101	Structure	93:101	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	52	dep	Forms	52:56	arg1	Synthesis					82:90	Synthesis	82:90	Synthesis	82:90	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	52	dep	Forms	52:56	arg1	Forms					52:56	Various Forms	44:56	Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity	44:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	52	dep	Forms	52:56	arg1	Activity					119:126	Biological Activity	108:126	Biological Activity	108:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	2	53	theme	Ag-containing	310:322	arg1	nanocomposites					324:337	New Ag-containing nanocomposites	306:337	New Ag-containing nanocomposites	306:337	New Ag-containing nanocomposites were obtained by modifying various forms of BC with Ag nanoparticles prepared by metal-vapor synthesis (MVS).
37108827	1	54	theme	materials	212:220	arg1	production					190:199	the production	186:199	the production of medical materials	186:220	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	9	55	theme	original	1519:1526	arg1	celluloses					1546:1555	the original and Ag-containing celluloses	1515:1555	the original and Ag-containing celluloses	1515:1555	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	10	56	theme	Ag	1745:1746	arg1	spectra					1751:1757	Ag 3d spectra	1745:1757	Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region	1745:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	6	57	dep	microscopy	1106:1115	arg1	SEM					1123:1125	SEM	1123:1125	SEM	1123:1125	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	57	dep	microscopy	1106:1115	arg1	TEM					1118:1120	TEM	1118:1120	TEM	1118:1120	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	3	58	theme	films	497:501	arg1	form					489:492	the form	485:492	the form of films (BCF) and spherical BC beads (SBCB)	485:537	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	9	59	theme	Ag-containing	1532:1544	arg1	celluloses					1546:1555	the original and Ag-containing celluloses	1515:1555	the original and Ag-containing celluloses	1515:1555	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	10	60	theme	large	1774:1778	arg1	proportion					1780:1789	a large proportion	1772:1789	a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region	1772:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	14	61	theme	application	2364:2374	arg1	possibility					2335:2345	the possibility	2331:2345	the possibility of their medical application	2331:2374	These results increase the possibility of their medical application.
37108827	6	62	theme	photoelectron	1207:1219	arg1	XPS					1235:1237	XPS	1235:1237	XPS	1235:1237	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	62	theme	photoelectron	1207:1219	arg1	spectroscopy					1221:1232	X-ray photoelectron spectroscopy	1201:1232	X-ray photoelectron spectroscopy (XPS)	1201:1238	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	7	63	theme	surface-sensitive	1381:1397	arg1	method					1399:1404	a surface-sensitive method	1379:1404	a surface-sensitive method	1379:1404	Since antimicrobial activity is largely determined by the surface composition, much attention was paid to studying its properties by XPS, a surface-sensitive method, at a sampling depth about 10 nm.
37108827	7	63	theme	surface-sensitive	1381:1397	arg1	XPS					1374:1376	XPS	1374:1376	XPS	1374:1376	Since antimicrobial activity is largely determined by the surface composition, much attention was paid to studying its properties by XPS, a surface-sensitive method, at a sampling depth about 10 nm.
37108827	11	64	theme	zerovalent	1935:1944	arg1	state					1946:1950	the zerovalent state	1931:1950	the zerovalent state	1931:1950	Ag NPs in the BC films and spherical beads were mainly in the zerovalent state.
37108827	6	65	theme	powder	1129:1134	arg1	XRD					1155:1157	XRD	1155:1157	XRD	1155:1157	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	65	theme	powder	1129:1134	arg1	diffraction					1142:1152	powder X-ray diffraction	1129:1152	powder X-ray diffraction (XRD)	1129:1158	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	10	66	theme	nanoparticles	1801:1813	arg1	proportion					1780:1789	a large proportion	1772:1789	a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region	1772:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	12	67	theme	BC-based	1953:1960	arg1	nanocomposites					1962:1975	BC-based nanocomposites	1953:1975	BC-based nanocomposites with Ag nanoparticles	1953:1997	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
37108827	1	68	theme	resources	172:180	arg1	use					155:157	the use	151:157	the use of renewable resources for the production of medical materials	151:220	Sustained interest in the use of renewable resources for the production of medical materials has stimulated research on bacterial cellulose (BC) and nanocomposites based on it.
37108827	9	69	from	intensity	1583:1591	arg1	latter					1622:1627	latter	1622:1627	latter	1622:1627	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	4	70	theme	Ag	631:632	arg1	nanoparticles					634:646	The Ag nanoparticles	627:646	The Ag nanoparticles synthesized in 2-propanol	627:672	The Ag nanoparticles synthesized in 2-propanol were incorporated into the polymer matrix using metal-containing organosol.
37108827	3	71	theme	BC	523:524	arg1	beads					526:530	spherical BC beads	513:530	spherical BC beads (SBCB)	513:537	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	3	71	theme	BC	523:524	arg1	SBCB					533:536	SBCB	533:536	SBCB	533:536	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	9	72	theme	C-C/C-H	1600:1606	arg1	groups					1608:1613	the C-C/C-H groups	1596:1613	the C-C/C-H groups	1596:1613	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	10	73	with	nanoparticles	1801:1813	arg1	size					1822:1825	a size	1820:1825	a size of less than 3 nm in the near-surface region	1820:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	13	74	theme	AgNPs/SBCB	2176:2185	arg1	nanocomposites					2187:2200	AgNPs/SBCB nanocomposites	2176:2200	AgNPs/SBCB nanocomposites	2176:2200	It was found that AgNPs/SBCB nanocomposites are more active than Ag NPs/BCF samples, especially against Candida albicans and Aspergillus niger fungi.
37108827	6	75	theme	X-ray	1173:1177	arg1	SAXS					1191:1194	SAXS	1191:1194	SAXS	1191:1194	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	6	75	theme	X-ray	1173:1177	arg1	scattering					1179:1188	small-angle X-ray scattering	1161:1188	small-angle X-ray scattering (SAXS)	1161:1195	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	10	76	theme	nm	1842:1843	arg1	size					1822:1825	a size	1820:1825	a size of less than 3 nm in the near-surface region	1820:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	5	77	theme	organic	880:886	arg1	substances					888:897	organic substances	880:897	organic substances during their co-condensation on the cooled walls of a reaction vessel	880:967	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	13	78	theme	Ag	2223:2224	arg1	samples					2234:2240	Ag NPs/BCF samples	2223:2240	Ag NPs/BCF samples	2223:2240	It was found that AgNPs/SBCB nanocomposites are more active than Ag NPs/BCF samples, especially against Candida albicans and Aspergillus niger fungi.
37108827	11	79	theme	Ag	1873:1874	arg1	NPs					1876:1878	Ag NPs	1873:1878	Ag NPs in the BC films and spherical beads	1873:1914	Ag NPs in the BC films and spherical beads were mainly in the zerovalent state.
37108827	7	80	theme	surface	1299:1305	arg1	composition					1307:1317	the surface composition	1295:1317	the surface composition	1295:1317	Since antimicrobial activity is largely determined by the surface composition, much attention was paid to studying its properties by XPS, a surface-sensitive method, at a sampling depth about 10 nm.
37108827	0	81	theme	Cellulose	71:79	arg1	Structure					93:101	Structure	93:101	Structure	93:101	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	81	theme	Cellulose	71:79	arg1	Synthesis					82:90	Synthesis	82:90	Synthesis	82:90	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	81	theme	Cellulose	71:79	arg1	Forms					52:56	Various Forms	44:56	Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity	44:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	81	theme	Cellulose	71:79	arg1	Activity					119:126	Biological Activity	108:126	Biological Activity	108:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	3	82	theme	hansenii	564:571	arg1	strain					583:588	the Gluconacetobacter hansenii GH-1/2008 strain	542:588	the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions	542:624	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	5	83	from	pressure	855:862	arg1	evaporation					828:838	evaporation	828:838	evaporation in vacuum at a pressure of 10-2 Pa	828:873	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	10	84	from	size	1822:1825	arg1	region					1865:1870	the near-surface region	1848:1870	the near-surface region	1848:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	5	85	theme	Pa	872:873	arg1	pressure					855:862	a pressure	853:862	a pressure of 10-2 Pa	853:873	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	9	86	theme	carbon	1656:1661	arg1	shell					1663:1667	carbon shell	1656:1667	carbon shell surrounding metal in Ag nanoparticles (Ag NPs)	1656:1714	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	13	87	theme	NPs/BCF	2226:2232	arg1	samples					2234:2240	Ag NPs/BCF samples	2223:2240	Ag NPs/BCF samples	2223:2240	It was found that AgNPs/SBCB nanocomposites are more active than Ag NPs/BCF samples, especially against Candida albicans and Aspergillus niger fungi.
37108827	8	88	theme	C	1440:1440	arg1	1s					1442:1443	C 1s	1440:1443	C 1s	1440:1443	C 1s and O 1s spectra were analyzed self-consistently.
37108827	2	89	theme	BC	383:384	arg1	forms					374:378	various forms	366:378	various forms of BC	366:384	New Ag-containing nanocomposites were obtained by modifying various forms of BC with Ag nanoparticles prepared by metal-vapor synthesis (MVS).
37108827	0	90	theme	Silver-Containing	7:23	arg1	Materials					25:33	Hybrid Silver-Containing Materials	0:33	Hybrid Silver-Containing Materials	0:33	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	7	91	theme	antimicrobial	1247:1259	arg1	activity					1261:1268	antimicrobial activity	1247:1268	antimicrobial activity	1247:1268	Since antimicrobial activity is largely determined by the surface composition, much attention was paid to studying its properties by XPS, a surface-sensitive method, at a sampling depth about 10 nm.
37108827	11	92	theme	BC	1887:1888	arg1	films					1890:1894	the BC films	1883:1894	the BC films	1883:1894	Ag NPs in the BC films and spherical beads were mainly in the zerovalent state.
37108827	8	93	theme	1s	1451:1452	arg1	spectra					1454:1460	O 1s spectra	1449:1460	O 1s spectra	1449:1460	C 1s and O 1s spectra were analyzed self-consistently.
37108827	5	94	theme	atomic	804:809	arg1	metals					811:816	extremely reactive atomic metals	785:816	extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa	785:873	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	13	95	theme	niger	2295:2299	arg1	fungi					2301:2305	Aspergillus niger fungi	2283:2305	Aspergillus niger fungi	2283:2305	It was found that AgNPs/SBCB nanocomposites are more active than Ag NPs/BCF samples, especially against Candida albicans and Aspergillus niger fungi.
37108827	2	96	theme	metal-vapor	420:430	arg1	MVS					443:445	MVS	443:445	MVS	443:445	New Ag-containing nanocomposites were obtained by modifying various forms of BC with Ag nanoparticles prepared by metal-vapor synthesis (MVS).
37108827	2	96	theme	metal-vapor	420:430	arg1	synthesis					432:440	metal-vapor synthesis	420:440	metal-vapor synthesis (MVS)	420:446	New Ag-containing nanocomposites were obtained by modifying various forms of BC with Ag nanoparticles prepared by metal-vapor synthesis (MVS).
37108827	3	97	theme	dynamic	607:613	arg1	conditions					615:624	static and dynamic conditions	596:624	static and dynamic conditions	596:624	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	4	98	theme	metal-containing	722:737	arg1	organosol					739:747	metal-containing organosol	722:747	metal-containing organosol	722:747	The Ag nanoparticles synthesized in 2-propanol were incorporated into the polymer matrix using metal-containing organosol.
37108827	2	99	theme	various	366:372	arg1	forms					374:378	various forms	366:378	various forms of BC	366:384	New Ag-containing nanocomposites were obtained by modifying various forms of BC with Ag nanoparticles prepared by metal-vapor synthesis (MVS).
37108827	0	100	theme	Various	44:50	arg1	Structure					93:101	Structure	93:101	Structure	93:101	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	100	theme	Various	44:50	arg1	Synthesis					82:90	Synthesis	82:90	Synthesis	82:90	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	100	theme	Various	44:50	arg1	Forms					52:56	Various Forms	44:56	Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity	44:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	0	100	theme	Various	44:50	arg1	Activity					119:126	Biological Activity	108:126	Biological Activity	108:126	Hybrid Silver-Containing Materials Based on Various Forms of Bacterial Cellulose: Synthesis, Structure, and Biological Activity.
37108827	9	101	theme	C	1499:1499	arg1	spectra					1504:1510	XPS C 1s spectra	1495:1510	XPS C 1s spectra of the original and Ag-containing celluloses	1495:1555	XPS C 1s spectra of the original and Ag-containing celluloses showed an increase in the intensity of the C-C/C-H groups in the latter, which are associated with carbon shell surrounding metal in Ag nanoparticles (Ag NPs).
37108827	3	102	theme	static	596:601	arg1	conditions					615:624	static and dynamic conditions	596:624	static and dynamic conditions	596:624	Bacterial cellulose was obtained in the form of films (BCF) and spherical BC beads (SBCB) by the Gluconacetobacter hansenii GH-1/2008 strain under static and dynamic conditions.
37108827	2	103	theme	Ag	391:392	arg1	nanoparticles					394:406	Ag nanoparticles	391:406	Ag nanoparticles prepared by metal-vapor synthesis (MVS)	391:446	New Ag-containing nanocomposites were obtained by modifying various forms of BC with Ag nanoparticles prepared by metal-vapor synthesis (MVS).
37108827	13	104	theme	Candida	2262:2268	arg1	albicans					2270:2277	Candida albicans	2262:2277	Candida albicans	2262:2277	It was found that AgNPs/SBCB nanocomposites are more active than Ag NPs/BCF samples, especially against Candida albicans and Aspergillus niger fungi.
37108827	12	105	with	nanocomposites	1962:1975	arg1	nanoparticles					1985:1997	Ag nanoparticles	1982:1997	Ag nanoparticles	1982:1997	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
37108827	5	106	theme	vessel	962:967	arg1	walls					942:946	the cooled walls	931:946	the cooled walls of a reaction vessel	931:967	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	6	107	theme	scanning	1088:1095	arg1	microscopy					1106:1115	transmission and scanning electron microscopy	1071:1115	microscopy	1106:1115	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	11	108	from	NPs	1876:1878	arg1	films					1890:1894	the BC films	1883:1894	the BC films	1883:1894	Ag NPs in the BC films and spherical beads were mainly in the zerovalent state.
37108827	11	108	from	NPs	1876:1878	arg1	beads					1910:1914	spherical beads	1900:1914	spherical beads	1900:1914	Ag NPs in the BC films and spherical beads were mainly in the zerovalent state.
37108827	10	109	theme	3d	1748:1749	arg1	spectra					1751:1757	Ag 3d spectra	1745:1757	Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region	1745:1870	The size effect observed in Ag 3d spectra evidenced on a large proportion of silver nanoparticles with a size of less than 3 nm in the near-surface region.
37108827	2	110	theme	New	306:308	arg1	nanocomposites					324:337	New Ag-containing nanocomposites	306:337	New Ag-containing nanocomposites	306:337	New Ag-containing nanocomposites were obtained by modifying various forms of BC with Ag nanoparticles prepared by metal-vapor synthesis (MVS).
37108827	5	111	from	co-condensation	912:926	arg1	walls					942:946	the cooled walls	931:946	the cooled walls of a reaction vessel	931:967	MVS is based on the interaction of extremely reactive atomic metals formed by evaporation in vacuum at a pressure of 10-2 Pa with organic substances during their co-condensation on the cooled walls of a reaction vessel.
37108827	6	112	theme	transmission	1071:1082	arg1	microscopy					1106:1115	transmission and scanning electron microscopy	1071:1115	microscopy	1106:1115	The composition, structure, and electronic state of the metal in the materials were characterized by transmission and scanning electron microscopy (TEM, SEM), powder X-ray diffraction (XRD), small-angle X-ray scattering (SAXS) and X-ray photoelectron spectroscopy (XPS).
37108827	12	113	theme	antimicrobial	2009:2021	arg1	activity					2023:2030	antimicrobial activity	2009:2030	antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi	2009:2155	BC-based nanocomposites with Ag nanoparticles exhibited antimicrobial activity against Bacillus subtilis, Staphylococcus aureus, Escherichia coli bacteria and Candida albicans and Aspergillus niger fungi.
35171342	0	0	theme	cellulose	83:91	arg1	digestibility					93:105	cellulose digestibility	83:105	cellulose digestibility	83:105	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	9	1	with	changes	1525:1531	arg1	fungi					1548:1552	white-rot fungi	1538:1552	white-rot fungi	1538:1552	• Feedstock changes with white-rot fungi favored the growth of other microorganisms.
35171342	1	2	theme	Miscanthus × giganteus	170:191	arg1	pretreatment					154:165	A sequential fungal pretreatment	134:165	A sequential fungal pretreatment of Miscanthus × giganteus	134:191	A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora.
35171342	6	3	from	degradation	1239:1249	arg1	system					1258:1263	the system	1254:1263	the system	1254:1263	Additionally, changes in the bacterial community occurred that might be associated with unwanted cellulose degradation in the system.
35171342	1	4	theme	fungus	295:300	arg1	subvermispora					316:328	the white-rot fungus Ceriporiopsis subvermispora	281:328	the white-rot fungus Ceriporiopsis subvermispora	281:328	A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora.
35171342	5	5	theme	microbial	857:865	arg1	community					867:875	A microbial community	855:875	A microbial community study	855:881	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	9	6	theme	white-rot	1538:1546	arg1	fungi					1548:1552	white-rot fungi	1538:1552	white-rot fungi	1538:1552	• Feedstock changes with white-rot fungi favored the growth of other microorganisms.
35171342	2	7	theme	fresh	418:422	arg1	Miscanthus					424:433	unsterilized fresh Miscanthus	405:433	unsterilized fresh Miscanthus	405:433	For three generations, each generation started with inoculation by mixing unsterilized fresh Miscanthus with end material from the previous generation and ended after 28 days of incubation at 28 °C.
35171342	1	8	theme	Ceriporiopsis	302:314	arg1	subvermispora					316:328	the white-rot fungus Ceriporiopsis subvermispora	281:328	the white-rot fungus Ceriporiopsis subvermispora	281:328	A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora.
35171342	10	9	with	pretreatment	1647:1658	arg1	subvermispora					1668:1680	C. subvermispora	1665:1680	C. subvermispora	1665:1680	• Feedstock microbial reduction is necessary for pretreatment with C. subvermispora.
35171342	3	10	theme	third	679:683	arg1	generations					685:695	the second and third generations	664:695	the second and third generations	664:695	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	8	11	theme	Miscanthus	1484:1493	arg1	pretreatment					1455:1466	• Sequential fungal pretreatment	1435:1466	• Sequential fungal pretreatment of unsterilized Miscanthus	1435:1493	KEY POINTS: • Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.
35171342	6	12	theme	cellulose	1229:1237	arg1	degradation					1239:1249	unwanted cellulose degradation	1220:1249	unwanted cellulose degradation in the system	1220:1263	Additionally, changes in the bacterial community occurred that might be associated with unwanted cellulose degradation in the system.
35171342	8	13	theme	Sequential	1437:1446	arg1	pretreatment					1455:1466	• Sequential fungal pretreatment	1435:1466	• Sequential fungal pretreatment of unsterilized Miscanthus	1435:1493	KEY POINTS: • Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.
35171342	5	14	dep	9	1094:1094	arg1	to					1086:1087	to	1086:1087	to	1086:1087	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	7	15	theme	load	1319:1322	arg1	reduction					1324:1332	feedstock microbial load reduction	1299:1332	feedstock microbial load reduction	1299:1332	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	0	16	from	changes	59:65	arg1	composition					70:80	composition	70:80	composition	70:80	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	0	16	from	changes	59:65	arg1	digestibility					93:105	cellulose digestibility	83:105	cellulose digestibility	83:105	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	0	16	from	changes	59:65	arg1	communities					121:131	microbial communities	111:131	microbial communities	111:131	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	6	17	theme	unwanted	1220:1227	arg1	degradation					1239:1249	unwanted cellulose degradation	1220:1249	unwanted cellulose degradation in the system	1220:1263	Additionally, changes in the bacterial community occurred that might be associated with unwanted cellulose degradation in the system.
35171342	5	18	dep	%	1095:1095	arg1	composed					990:997	composed	990:997	was composed mainly of this fungus (> 99%)	986:1027	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	3	19	theme	cellulose	562:570	arg1	digestibility					572:584	the cellulose digestibility	558:584	the cellulose digestibility of the material	558:600	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	4	20	theme	structural	792:801	arg1	carbohydrates					803:815	Miscanthus structural carbohydrates	781:815	Miscanthus structural carbohydrates	781:815	Furthermore, high degradation of Miscanthus structural carbohydrates occurred during the first generation.
35171342	4	21	theme	Miscanthus	781:790	arg1	carbohydrates					803:815	Miscanthus structural carbohydrates	781:815	Miscanthus structural carbohydrates	781:815	Furthermore, high degradation of Miscanthus structural carbohydrates occurred during the first generation.
35171342	3	22	theme	material	593:600	arg1	digestibility					572:584	the cellulose digestibility	558:584	the cellulose digestibility of the material	558:600	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	10	23	theme	•	1598:1598	arg1	reduction					1620:1628	• Feedstock microbial reduction	1598:1628	• Feedstock microbial reduction	1598:1628	• Feedstock microbial reduction is necessary for pretreatment with C. subvermispora.
35171342	2	24	theme	previous	462:469	arg1	generation					471:480	the previous generation	458:480	the previous generation	458:480	For three generations, each generation started with inoculation by mixing unsterilized fresh Miscanthus with end material from the previous generation and ended after 28 days of incubation at 28 °C.
35171342	0	25	theme	microbial	111:119	arg1	communities					121:131	microbial communities	111:131	microbial communities	111:131	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	5	26	theme	community	867:875	arg1	study					877:881	A microbial community study	855:881	A microbial community study	855:881	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	0	27	theme	Sequential	0:9	arg1	pretreatment					18:29	Sequential fungal pretreatment	0:29	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.	0:132	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	7	28	theme	lignocellulosic	1398:1412	arg1	biomass					1414:1420	lignocellulosic biomass	1398:1420	lignocellulosic biomass	1398:1420	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	0	29	theme	fungal	11:16	arg1	pretreatment					18:29	Sequential fungal pretreatment	0:29	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.	0:132	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	7	30	theme	reduction	1324:1332	arg1	necessity					1286:1294	the necessity	1282:1294	the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass	1282:1420	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	7	31	theme	fungal	1375:1380	arg1	pretreatment					1382:1393	fungal pretreatment	1375:1393	fungal pretreatment of lignocellulosic biomass	1375:1420	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	2	32	theme	incubation	509:518	arg1	28 days					498:504	28 days	498:504	28 days of incubation	498:518	For three generations, each generation started with inoculation by mixing unsterilized fresh Miscanthus with end material from the previous generation and ended after 28 days of incubation at 28 °C.
35171342	8	33	dep	POINTS	1427:1432	arg1	unsuccessful					1499:1510	unsuccessful	1499:1510	was unsuccessful	1495:1510	KEY POINTS: • Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.
35171342	5	34	theme	material	936:943	arg1	community					919:927	the fungal community	908:927	the fungal community of the material previously colonized by C. subvermispora	908:984	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	5	35	dep	composed	990:997	arg1	%					1026:1026	> 99%	1022:1026	> 99%	1022:1026	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	1	36	theme	unsterilized	217:228	arg1	Miscanthus					230:239	unsterilized Miscanthus	217:239	unsterilized Miscanthus	217:239	A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora.
35171342	7	37	theme	biomass	1414:1420	arg1	pretreatment					1382:1393	fungal pretreatment	1375:1393	fungal pretreatment of lignocellulosic biomass	1375:1420	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	9	38	theme	Feedstock	1515:1523	arg1	changes					1525:1531	• Feedstock changes	1513:1531	• Feedstock changes with white-rot fungi	1513:1552	• Feedstock changes with white-rot fungi favored the growth of other microorganisms.
35171342	4	39	theme	first	837:841	arg1	generation					843:852	the first generation	833:852	the first generation	833:852	Furthermore, high degradation of Miscanthus structural carbohydrates occurred during the first generation.
35171342	5	40	theme	fungal	912:917	arg1	community					919:927	the fungal community	908:927	the fungal community of the material previously colonized by C. subvermispora	908:984	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	9	41	theme	microorganisms	1582:1595	arg1	growth					1566:1571	the growth	1562:1571	the growth of other microorganisms	1562:1595	• Feedstock changes with white-rot fungi favored the growth of other microorganisms.
35171342	5	42	theme	other	1102:1106	arg1	microbes					1108:1115	other microbes	1102:1115	other microbes	1102:1115	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	4	43	theme	high	761:764	arg1	degradation					766:776	high degradation	761:776	high degradation of Miscanthus structural carbohydrates	761:815	Furthermore, high degradation of Miscanthus structural carbohydrates occurred during the first generation.
35171342	9	44	theme	•	1513:1513	arg1	changes					1525:1531	• Feedstock changes	1513:1531	• Feedstock changes with white-rot fungi	1513:1552	• Feedstock changes with white-rot fungi favored the growth of other microorganisms.
35171342	7	45	theme	feedstock	1299:1307	arg1	reduction					1324:1332	feedstock microbial load reduction	1299:1332	feedstock microbial load reduction	1299:1332	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	0	46	theme	Miscanthus	47:56	arg1	pretreatment					18:29	Sequential fungal pretreatment	0:29	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.	0:132	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	6	47	theme	bacterial	1161:1169	arg1	community					1171:1179	the bacterial community	1157:1179	the bacterial community	1157:1179	Additionally, changes in the bacterial community occurred that might be associated with unwanted cellulose degradation in the system.
35171342	9	48	theme	other	1576:1580	arg1	microorganisms					1582:1595	other microorganisms	1576:1595	other microorganisms	1576:1595	• Feedstock changes with white-rot fungi favored the growth of other microorganisms.
35171342	7	49	theme	pretreatment	1382:1393	arg1	reproducibility					1356:1370	reproducibility	1356:1370	reproducibility	1356:1370	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	7	49	theme	pretreatment	1382:1393	arg1	stability					1342:1350	stability	1342:1350	stability	1342:1350	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	0	50	theme	unsterilized	34:45	arg1	Miscanthus					47:56	unsterilized Miscanthus	34:56	unsterilized Miscanthus	34:56	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	3	51	from	enhancements	707:718	arg1	digestibility					733:745	cellulose digestibility	723:745	cellulose digestibility	723:745	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	3	52	theme	unsterilized	635:646	arg1	Miscanthus					648:657	the unsterilized Miscanthus	631:657	the unsterilized Miscanthus	631:657	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	2	53	theme	unsterilized	405:416	arg1	Miscanthus					424:433	unsterilized fresh Miscanthus	405:433	unsterilized fresh Miscanthus	405:433	For three generations, each generation started with inoculation by mixing unsterilized fresh Miscanthus with end material from the previous generation and ended after 28 days of incubation at 28 °C.
35171342	10	54	theme	microbial	1610:1618	arg1	reduction					1620:1628	• Feedstock microbial reduction	1598:1628	• Feedstock microbial reduction	1598:1628	• Feedstock microbial reduction is necessary for pretreatment with C. subvermispora.
35171342	8	55	theme	unsterilized	1471:1482	arg1	Miscanthus					1484:1493	unsterilized Miscanthus	1471:1493	unsterilized Miscanthus	1471:1493	KEY POINTS: • Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.
35171342	4	56	theme	carbohydrates	803:815	arg1	degradation					766:776	high degradation	761:776	high degradation of Miscanthus structural carbohydrates	761:815	Furthermore, high degradation of Miscanthus structural carbohydrates occurred during the first generation.
35171342	10	57	theme	Feedstock	1600:1608	arg1	reduction					1620:1628	• Feedstock microbial reduction	1598:1628	• Feedstock microbial reduction	1598:1628	• Feedstock microbial reduction is necessary for pretreatment with C. subvermispora.
35171342	7	58	dep	stability	1342:1350	arg1	the					1338:1340	the	1338:1340	the	1338:1340	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	1	59	theme	sequential	136:145	arg1	pretreatment					154:165	A sequential fungal pretreatment	134:165	A sequential fungal pretreatment of Miscanthus × giganteus	134:191	A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora.
35171342	8	60	theme	KEY	1423:1425	arg1	POINTS					1427:1432	KEY POINTS	1423:1432	KEY POINTS: • Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.	1423:1511	KEY POINTS: • Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.
35171342	5	61	theme	first	1037:1041	arg1	generation					1043:1052	the first generation	1033:1052	the first generation	1033:1052	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	0	62	dep	pretreatment	18:29	arg1	changes					59:65	changes	59:65	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.	0:132	Sequential fungal pretreatment of unsterilized Miscanthus: changes in composition, cellulose digestibility and microbial communities.
35171342	1	63	theme	fungal	147:152	arg1	pretreatment					154:165	A sequential fungal pretreatment	134:165	A sequential fungal pretreatment of Miscanthus × giganteus	134:191	A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora.
35171342	3	64	theme	first	540:544	arg1	generation					546:555	the first generation	536:555	the first generation	536:555	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	3	65	theme	cellulose	723:731	arg1	digestibility					733:745	cellulose digestibility	723:745	cellulose digestibility	723:745	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	5	66	theme	relative	1058:1065	arg1	abundance					1067:1075	its relative abundance	1054:1075	its relative abundance	1054:1075	A microbial community study showed that, even though the fungal community of the material previously colonized by C. subvermispora was composed mainly of this fungus (> 99%), by the first generation its relative abundance was down to only 9%, and other microbes had prevailed.
35171342	2	67	from	generation	471:480	arg1	material					444:451	end material	440:451	end material from the previous generation	440:480	For three generations, each generation started with inoculation by mixing unsterilized fresh Miscanthus with end material from the previous generation and ended after 28 days of incubation at 28 °C.
35171342	2	68	theme	end	440:442	arg1	material					444:451	end material	440:451	end material from the previous generation	440:480	For three generations, each generation started with inoculation by mixing unsterilized fresh Miscanthus with end material from the previous generation and ended after 28 days of incubation at 28 °C.
35171342	8	69	theme	fungal	1448:1453	arg1	pretreatment					1455:1466	• Sequential fungal pretreatment	1435:1466	• Sequential fungal pretreatment of unsterilized Miscanthus	1435:1493	KEY POINTS: • Sequential fungal pretreatment of unsterilized Miscanthus was unsuccessful.
35171342	3	70	theme	second	668:673	arg1	generations					685:695	the second and third generations	664:695	the second and third generations	664:695	After the first generation, the cellulose digestibility of the material doubled, compared to that of the unsterilized Miscanthus, but the second and third generations showed no enhancements in cellulose digestibility.
35171342	6	71	from	changes	1146:1152	arg1	community					1171:1179	the bacterial community	1157:1179	the bacterial community	1157:1179	Additionally, changes in the bacterial community occurred that might be associated with unwanted cellulose degradation in the system.
35171342	7	72	theme	microbial	1309:1317	arg1	reduction					1324:1332	feedstock microbial load reduction	1299:1332	feedstock microbial load reduction	1299:1332	This reiterates the necessity of feedstock microbial load reduction for the stability and reproducibility of fungal pretreatment of lignocellulosic biomass.
35171342	1	73	theme	white-rot	285:293	arg1	subvermispora					316:328	the white-rot fungus Ceriporiopsis subvermispora	281:328	the white-rot fungus Ceriporiopsis subvermispora	281:328	A sequential fungal pretreatment of Miscanthus × giganteus was conducted by mixing unsterilized Miscanthus with material previously colonized with the white-rot fungus Ceriporiopsis subvermispora.
35075504	6	0	theme	major	1083:1087	arg1	muscle					1089:1094	the pectoralis major muscle	1068:1094	the pectoralis major muscle based on the dimensions of the implant	1068:1133	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	9	1	theme	satisfaction	1630:1641	arg1	resolution					1606:1615	resolution	1606:1615	resolution of symptoms, satisfaction, and complications	1606:1660	Patients were evaluated in terms of resolution of symptoms, satisfaction, and complications.
35075504	6	2	theme	points/dermal	1164:1176	arg1	sutures					1185:1191	support points/dermal bridge sutures	1156:1191	support points/dermal bridge sutures	1156:1191	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	12	3	theme	volume	2129:2134	arg1	decrease					2136:2143	an average volume decrease	2118:2143	an average volume decrease of 120 cc	2118:2153	SmoothSilk surface silicone implants were used in all cases, with an average volume decrease of 120 cc.
35075504	6	4	theme	breast	1252:1257	arg1	pole					1259:1262	the lower breast pole	1242:1262	the lower breast pole (LBP)	1242:1268	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	6	4	theme	breast	1252:1257	arg1	LBP					1265:1267	LBP	1265:1267	LBP	1265:1267	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	0	5	with	Support	75:81	arg1	Variations					200:209	its Technical Variations	186:209	its Technical Variations	186:209	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	5	with	Support	75:81	arg1	CRIMS					175:179	CRIMS	175:179	CRIMS	175:179	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	5	with	Support	75:81	arg1	Sling					168:172	Composite Reverse Inferior Muscle Sling	134:172	Composite Reverse Inferior Muscle Sling (CRIMS)	134:180	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	15	6	theme	first	2488:2492	arg1	months					2496:2501	the first 3 months	2484:2501	the first 3 months	2484:2501	The value for LPS was 7.87% (p &lt;0.0001) between 10 days and 1 year, with the majority occurring early in the first 3 months, indicating that the LBP/implant remains steady during the last months of follow-up.
35075504	20	7	theme	decision-making	2934:2948	arg1	process					2950:2956	the pre- and intraoperative decision-making process	2906:2956	the pre- and intraoperative decision-making process	2906:2956	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	10	8	theme	intermammary	1830:1841	arg1	IMD					1853:1855	IMD	1853:1855	IMD	1853:1855	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	10	8	theme	intermammary	1830:1841	arg1	distance					1843:1850	intermammary distance	1830:1850	intermammary distance (IMD)	1830:1856	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	12	9	used	used	2094:2097	arg2	implants					2080:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants were used in all cases, with an average volume decrease of 120 cc.
35075504	5	10	theme	sling	886:890	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	4	11	theme	breast	684:689	arg1	augmentation					691:702	primary breast augmentation	676:702	primary breast augmentation	676:702	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	10	12	theme	scanner	1720:1726	arg1	system					1728:1733	the Divina scanner system	1709:1733	the Divina scanner system	1709:1733	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	23	13	theme	ratings	3337:3343	arg1	description					3292:3302	a full description	3285:3302	a full description of these Evidence-Based Medicine ratings	3285:3343	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
35075504	3	14	theme	surface	565:571	arg1	stability					529:537	stability	529:537	stability	529:537	These deformities can be addressed with various procedures, but the pocket control and stability of the new smooth implant surface may present limitations.
35075504	3	14	theme	surface	565:571	arg1	control					517:523	the pocket control	506:523	the pocket control	506:523	These deformities can be addressed with various procedures, but the pocket control and stability of the new smooth implant surface may present limitations.
35075504	5	15	theme	CRIMS	893:897	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	20	16	theme	acceptable	3020:3029	arg1	rates					3044:3048	acceptable complication rates	3020:3048	acceptable complication rates	3020:3048	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	6	17	with	combination	1139:1149	arg1	sutures					1185:1191	support points/dermal bridge sutures	1156:1191	support points/dermal bridge sutures	1156:1191	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	6	18	theme	implant	1127:1133	arg1	dimensions					1109:1118	the dimensions	1105:1118	the dimensions of the implant	1105:1133	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	0	19	theme	Implant	111:117	arg1	Stability					119:127	Smooth Implant Stability	104:127	Smooth Implant Stability	104:127	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	3	20	theme	smooth	550:555	arg1	surface					565:571	the new smooth implant surface	542:571	the new smooth implant surface	542:571	These deformities can be addressed with various procedures, but the pocket control and stability of the new smooth implant surface may present limitations.
35075504	5	21	theme	composite	852:860	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	20	22	theme	pocket	2978:2983	arg1	control					2985:2991	the new pocket control	2970:2991	the new pocket control	2970:2991	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	6	23	theme	glandular	1222:1230	arg1	tissue					1232:1237	glandular tissue	1222:1237	glandular tissue	1222:1237	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	4	24	theme	predictable	652:662	arg1	approach					664:671	a previously described predictable approach	629:671	a previously described predictable approach	629:671	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	10	25	used	used	1739:1742	arg2	3DI					1690:1692	3DI	1690:1692	3DI	1690:1692	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	10	25	used	used	1739:1742	arg2	imaging					1681:1687	Three-dimensional imaging	1663:1687	Three-dimensional imaging (3DI) obtained from the Divina scanner system	1663:1733	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	5	26	theme	inferior	870:877	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	18	27	theme	displacement	2753:2764	arg1	cases					2773:2777	no cases	2770:2777	no cases of rotation	2770:2789	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	18	27	theme	displacement	2753:2764	arg1	%					2731:2731	3%	2730:2731	3%	2730:2731	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	18	27	theme	displacement	2753:2764	arg1	cases					2723:2727	2 cases	2721:2727	2 cases (3%) of minimal implant displacement	2721:2764	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	4	28	theme	treatment algorithm for	727:749	arg1	selection					765:773	a surgical treatment algorithm for RAM technique selection	716:773	a surgical treatment algorithm for RAM technique selection	716:773	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	0	29	theme	Reverse	144:150	arg1	CRIMS					175:179	CRIMS	175:179	CRIMS	175:179	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	29	theme	Reverse	144:150	arg1	Sling					168:172	Composite Reverse Inferior Muscle Sling	134:172	Composite Reverse Inferior Muscle Sling (CRIMS)	134:180	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	13	30	with	satisfied/satisfied	2202:2220	arg1	result					2243:2248	their aesthetic result	2227:2248	their aesthetic result	2227:2248	Sixty-eight patients (94.4%) were either very satisfied/satisfied with their aesthetic result.
35075504	8	31	theme	34	1539:1540	arg1	months					1381:1386	at least 6 months	1370:1386	at least 6 months in 5 cases (6.9%)	1370:1404	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	31	theme	34	1539:1540	arg1	months					1542:1547	mean 34 months	1534:1547	mean 34 months	1534:1547	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	15	32	dep	p	2405:2405	arg1	0.0001					2411:2416	0.0001	2411:2416	0.0001	2411:2416	The value for LPS was 7.87% (p &lt;0.0001) between 10 days and 1 year, with the majority occurring early in the first 3 months, indicating that the LBP/implant remains steady during the last months of follow-up.
35075504	18	33	theme	minimal	2737:2743	arg1	displacement					2753:2764	minimal implant displacement	2737:2764	minimal implant displacement	2737:2764	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	4	34	theme	technique	755:763	arg1	selection					765:773	a surgical treatment algorithm for RAM technique selection	716:773	a surgical treatment algorithm for RAM technique selection	716:773	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	8	35	from	months	1463:1468	arg1	%					1487:1487	13.8%	1483:1487	13.8%	1483:1487	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	35	from	months	1463:1468	arg1	cases					1476:1480	10 cases	1473:1480	10 cases (13.8%)	1473:1488	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	19	36	dep	CONCLUSIONS	2792:2802	arg1	variations					2818:2827	its variations	2814:2827	its variations	2814:2827	CONCLUSIONS CRIMS and its variations can be performed successfully in RAM.
35075504	19	36	dep	CONCLUSIONS	2792:2802	arg1	CRIMS					2804:2808	CRIMS	2804:2808	CRIMS	2804:2808	CONCLUSIONS CRIMS and its variations can be performed successfully in RAM.
35075504	11	37	from	scarring	1953:1960	arg1	dehiscence					1981:1990	wound dehiscence	1975:1990	wound dehiscence	1975:1990	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	37	from	scarring	1953:1960	arg1	4					1965:1965	4	1965:1965	4	1965:1965	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	37	from	scarring	1953:1960	arg1	%					1971:1971	5.5%	1968:1971	5.5%	1968:1971	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	37	from	scarring	1953:1960	arg1	4					1995:1995	4	1995:1995	4	1995:1995	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	37	from	scarring	1953:1960	arg1	%					2001:2001	5.5%	1998:2001	5.5%	1998:2001	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	5	38	theme	types	940:944	arg1	variations					928:937	its technical variations	914:937	its technical variations (types I-IV)	914:950	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	5	38	theme	types	940:944	arg1	I-IV					946:949	types I-IV	940:949	types I-IV	940:949	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	0	39	theme	Soft-Tissue	63:73	arg1	Support					75:81	Soft-Tissue Support	63:81	Soft-Tissue Support	63:81	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	14	40	located	observed	2366:2373	arg1	%					2337:2337	10.7%	2333:2337	10.7%	2333:2337	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	14	40	located	observed	2366:2373	arg1	breasts					2324:2330	7 breasts	2322:2330	7 breasts (10.7%)	2322:2338	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	14	40	located	observed	2366:2373	arg2	cysts					2355:2359	localized oil cysts	2341:2359	localized oil cysts	2341:2359	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	11	41	dep	RESULTS	1859:1865	arg1	observed					1908:1915	observed	1908:1915	were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%)	1903:2049	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	0	42	theme	Muscle	161:166	arg1	CRIMS					175:179	CRIMS	175:179	CRIMS	175:179	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	42	theme	Muscle	161:166	arg1	Sling					168:172	Composite Reverse Inferior Muscle Sling	134:172	Composite Reverse Inferior Muscle Sling (CRIMS)	134:180	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	10	43	theme	breast	1783:1788	arg1	position					1790:1797	breast position	1783:1797	breast position	1783:1797	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	15	44	dep	%	2402:2402	arg1	p					2405:2405	p &lt	2405:2409	p &lt;0.0001	2405:2416	The value for LPS was 7.87% (p &lt;0.0001) between 10 days and 1 year, with the majority occurring early in the first 3 months, indicating that the LBP/implant remains steady during the last months of follow-up.
35075504	12	45	theme	cc	2152:2153	arg1	decrease					2136:2143	an average volume decrease	2118:2143	an average volume decrease of 120 cc	2118:2153	SmoothSilk surface silicone implants were used in all cases, with an average volume decrease of 120 cc.
35075504	11	46	theme	II/III	2011:2016	arg1	patients					1922:1929	9 patients	1920:1929	9 patients (12.5%)	1920:1937	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	46	theme	II/III	2011:2016	arg1	contracture					2027:2037	Baker II/III capsular contracture	2005:2037	Baker II/III capsular contracture	2005:2037	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	21	47	theme	Further	3051:3057	arg1	evaluation					3068:3077	Further accurate evaluation	3051:3077	Further accurate evaluation	3051:3077	Further accurate evaluation is recommended to understand the benefits or disadvantages of CRIMS compared to other RAM techniques.
35075504	17	48	theme	intra/extracapsular	2658:2676	arg1	ruptures					2678:2685	intra/extracapsular ruptures	2658:2685	intra/extracapsular ruptures	2658:2685	There were no signs of intra/extracapsular ruptures, capsular contracture.
35075504	6	49	theme	silicone	988:995	arg1	implant					1001:1007	a silicone gel implant	986:1007	a silicone gel implant	986:1007	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	23	50	theme	full	3287:3290	arg1	description					3292:3302	a full description	3285:3302	a full description of these Evidence-Based Medicine ratings	3285:3343	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
35075504	13	51	theme	Sixty-eight	2156:2166	arg1	patients					2168:2175	Sixty-eight patients	2156:2175	Sixty-eight patients (94.4%)	2156:2183	Sixty-eight patients (94.4%) were either very satisfied/satisfied with their aesthetic result.
35075504	13	51	theme	Sixty-eight	2156:2166	arg1	%					2182:2182	94.4%	2178:2182	94.4%	2178:2182	Sixty-eight patients (94.4%) were either very satisfied/satisfied with their aesthetic result.
35075504	4	52	from	revisits	620:627	arg1	augmentation					691:702	primary breast augmentation	676:702	primary breast augmentation	676:702	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	21	53	theme	CRIMS	3141:3145	arg1	disadvantages					3124:3136	disadvantages	3124:3136	disadvantages	3124:3136	Further accurate evaluation is recommended to understand the benefits or disadvantages of CRIMS compared to other RAM techniques.
35075504	21	53	theme	CRIMS	3141:3145	arg1	benefits					3112:3119	benefits	3112:3119	benefits	3112:3119	Further accurate evaluation is recommended to understand the benefits or disadvantages of CRIMS compared to other RAM techniques.
35075504	0	54	theme	Reoperative	0:10	arg1	Mammoplasty					25:35	Reoperative Augmentation Mammoplasty	0:35	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.	0:210	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	6	55	theme	lower	1246:1250	arg1	pole					1259:1262	the lower breast pole	1242:1262	the lower breast pole (LBP)	1242:1268	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	6	55	theme	lower	1246:1250	arg1	LBP					1265:1267	LBP	1265:1267	LBP	1265:1267	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	6	56	theme	inferior	1050:1057	arg1	sling					1059:1063	an inferior sling	1047:1063	an inferior sling of the pectoralis major muscle based on the dimensions of the implant	1047:1133	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	20	57	theme	algorithmic	2870:2880	arg1	approach					2882:2889	An algorithmic approach	2867:2889	An algorithmic approach	2867:2889	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	22	58	dep	EVIDENCE	3190:3197	arg1	journal					3207:3213	This journal	3202:3213	EVIDENCE IV This journal	3190:3213	LEVEL OF EVIDENCE IV This journal requires that authors assign a level of evidence to each article.
35075504	12	59	theme	surface	2063:2069	arg1	implants					2080:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants were used in all cases, with an average volume decrease of 120 cc.
35075504	11	60	dep	patients	1922:1929	arg1	scarring					1953:1960	hypertrophic scarring	1940:1960	hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%)	1940:2002	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	14	61	theme	patients	2297:2304	arg1	group					2285:2289	a group	2283:2289	a group of 65 patients (90.2%)	2283:2312	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	14	61	theme	patients	2297:2304	arg1	patients					2297:2304	65 patients	2294:2304	65 patients (90.2%)	2294:2312	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	14	61	theme	patients	2297:2304	arg1	%					2311:2311	90.2%	2307:2311	90.2%	2307:2311	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	11	62	theme	minor	1883:1887	arg1	complications					1889:1901	minor complications	1883:1901	minor complications	1883:1901	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	8	63	from	months	1508:1513	arg1	cases					1432:1436	50 cases	1429:1436	50 cases (69.4%)	1429:1444	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	63	from	months	1508:1513	arg1	%					1443:1443	69.4%	1439:1443	69.4%	1439:1443	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	63	from	months	1508:1513	arg1	cases					1393:1397	5 cases	1391:1397	5 cases (6.9%)	1391:1404	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	63	from	months	1508:1513	arg1	%					1403:1403	6.9%	1400:1403	6.9%	1400:1403	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	63	from	months	1508:1513	arg1	cases					1520:1524	7 cases	1518:1524	7 cases	1518:1524	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	1	64	theme	highest	303:309	arg1	rates					311:315	the highest rates	299:315	the highest rates of complications and revision	299:345	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	10	65	theme	pole	1806:1809	arg1	LPS					1820:1822	LPS	1820:1822	LPS	1820:1822	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	10	65	theme	pole	1806:1809	arg1	stretch					1811:1817	lower pole stretch	1800:1817	lower pole stretch (LPS)	1800:1823	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	1	66	theme	BACKGROUND	212:221	arg1	procedure					283:291	a challenging procedure	269:291	a challenging procedure	269:291	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	1	66	theme	BACKGROUND	212:221	arg1	RAM					261:263	RAM	261:263	RAM	261:263	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	1	66	theme	BACKGROUND	212:221	arg1	mammoplasty					248:258	BACKGROUND Reoperative augmentation mammoplasty	212:258	BACKGROUND Reoperative augmentation mammoplasty (RAM)	212:264	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	23	67	theme	online	3391:3396	arg1	Instructions					3398:3409	the online Instructions	3387:3409	the online Instructions to Authors www.springer.com/00266	3387:3443	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
35075504	3	68	theme	pocket	510:515	arg1	control					517:523	the pocket control	506:523	the pocket control	506:523	These deformities can be addressed with various procedures, but the pocket control and stability of the new smooth implant surface may present limitations.
35075504	1	69	theme	augmentation	235:246	arg1	procedure					283:291	a challenging procedure	269:291	a challenging procedure	269:291	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	1	69	theme	augmentation	235:246	arg1	RAM					261:263	RAM	261:263	RAM	261:263	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	1	69	theme	augmentation	235:246	arg1	mammoplasty					248:258	BACKGROUND Reoperative augmentation mammoplasty	212:258	BACKGROUND Reoperative augmentation mammoplasty (RAM)	212:264	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	6	70	theme	muscle	1089:1094	arg1	sling					1059:1063	an inferior sling	1047:1063	an inferior sling of the pectoralis major muscle based on the dimensions of the implant	1047:1133	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	12	71	theme	average	2121:2127	arg1	decrease					2136:2143	an average volume decrease	2118:2143	an average volume decrease of 120 cc	2118:2153	SmoothSilk surface silicone implants were used in all cases, with an average volume decrease of 120 cc.
35075504	10	72	theme	Three-dimensional	1663:1679	arg1	3DI					1690:1692	3DI	1690:1692	3DI	1690:1692	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	10	72	theme	Three-dimensional	1663:1679	arg1	imaging					1681:1687	Three-dimensional imaging	1663:1687	Three-dimensional imaging (3DI) obtained from the Divina scanner system	1663:1733	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	16	73	theme	fat	2600:2602	arg1	necrosis/seroma					2604:2618	fat necrosis/seroma	2600:2618	fat necrosis/seroma	2600:2618	No cases of fat necrosis/seroma were observed.
35075504	6	74	theme	pectoralis	1072:1081	arg1	muscle					1089:1094	the pectoralis major muscle	1068:1094	the pectoralis major muscle based on the dimensions of the implant	1068:1133	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	23	75	theme	Evidence-Based	3313:3326	arg1	ratings					3337:3343	these Evidence-Based Medicine ratings	3307:3343	these Evidence-Based Medicine ratings	3307:3343	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
35075504	20	76	theme	pre-	2910:2913	arg1	process					2950:2956	the pre- and intraoperative decision-making process	2906:2956	the pre- and intraoperative decision-making process	2906:2956	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	6	77	theme	support	1156:1162	arg1	sutures					1185:1191	support points/dermal bridge sutures	1156:1191	support points/dermal bridge sutures	1156:1191	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	9	78	theme	complications	1648:1660	arg1	resolution					1606:1615	resolution	1606:1615	resolution of symptoms, satisfaction, and complications	1606:1660	Patients were evaluated in terms of resolution of symptoms, satisfaction, and complications.
35075504	0	79	theme	Technical	190:198	arg1	Variations					200:209	its Technical Variations	186:209	its Technical Variations	186:209	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	5	80	theme	muscle	879:884	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	20	81	theme	intraoperative	2919:2932	arg1	process					2950:2956	the pre- and intraoperative decision-making process	2906:2956	the pre- and intraoperative decision-making process	2906:2956	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	1	82	with	procedure	283:291	arg1	rates					311:315	the highest rates	299:315	the highest rates of complications and revision	299:345	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	11	83	theme	hypertrophic	1940:1951	arg1	scarring					1953:1960	hypertrophic scarring	1940:1960	hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%)	1940:2002	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	21	84	theme	other	3159:3163	arg1	techniques					3169:3178	other RAM techniques	3159:3178	other RAM techniques	3159:3178	Further accurate evaluation is recommended to understand the benefits or disadvantages of CRIMS compared to other RAM techniques.
35075504	3	85	theme	implant	557:563	arg1	surface					565:571	the new smooth implant surface	542:571	the new smooth implant surface	542:571	These deformities can be addressed with various procedures, but the pocket control and stability of the new smooth implant surface may present limitations.
35075504	14	86	theme	Breast	2251:2256	arg1	images					2258:2263	Breast images	2251:2263	Breast images	2251:2263	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	18	87	theme	rotation	2782:2789	arg1	cases					2773:2777	no cases	2770:2777	no cases of rotation	2770:2789	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	18	87	theme	rotation	2782:2789	arg1	%					2731:2731	3%	2730:2731	3%	2730:2731	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	18	87	theme	rotation	2782:2789	arg1	cases					2723:2727	2 cases	2721:2727	2 cases (3%) of minimal implant displacement	2721:2764	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	5	88	with	RAM	843:845	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	4	89	theme	primary	676:682	arg1	augmentation					691:702	primary breast augmentation	676:702	primary breast augmentation	676:702	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	10	90	theme	Divina	1713:1718	arg1	system					1728:1733	the Divina scanner system	1709:1733	the Divina scanner system	1709:1733	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	0	91	with	Control	91:97	arg1	Variations					200:209	its Technical Variations	186:209	its Technical Variations	186:209	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	91	with	Control	91:97	arg1	CRIMS					175:179	CRIMS	175:179	CRIMS	175:179	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	91	with	Control	91:97	arg1	Sling					168:172	Composite Reverse Inferior Muscle Sling	134:172	Composite Reverse Inferior Muscle Sling (CRIMS)	134:180	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	3	92	theme	new	546:548	arg1	surface					565:571	the new smooth implant surface	542:571	the new smooth implant surface	542:571	These deformities can be addressed with various procedures, but the pocket control and stability of the new smooth implant surface may present limitations.
35075504	15	93	theme	last	2562:2565	arg1	months					2567:2572	the last months	2558:2572	the last months of follow-up	2558:2585	The value for LPS was 7.87% (p &lt;0.0001) between 10 days and 1 year, with the majority occurring early in the first 3 months, indicating that the LBP/implant remains steady during the last months of follow-up.
35075504	14	94	theme	oil	2351:2353	arg1	cysts					2355:2359	localized oil cysts	2341:2359	localized oil cysts	2341:2359	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	0	95	theme	Composite	134:142	arg1	CRIMS					175:179	CRIMS	175:179	CRIMS	175:179	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	95	theme	Composite	134:142	arg1	Sling					168:172	Composite Reverse Inferior Muscle Sling	134:172	Composite Reverse Inferior Muscle Sling (CRIMS)	134:180	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	5	96	theme	reverse	862:868	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	20	97	theme	new	2974:2976	arg1	control					2985:2991	the new pocket control	2970:2991	the new pocket control	2970:2991	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	6	98	theme	CRIMS	953:957	arg1	technique					959:967	CRIMS technique	953:967	CRIMS technique	953:967	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	4	99	theme	described	642:650	arg1	approach					664:671	a previously described predictable approach	629:671	a previously described predictable approach	629:671	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	0	100	theme	Inferior	152:159	arg1	CRIMS					175:179	CRIMS	175:179	CRIMS	175:179	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	100	theme	Inferior	152:159	arg1	Sling					168:172	Composite Reverse Inferior Muscle Sling	134:172	Composite Reverse Inferior Muscle Sling (CRIMS)	134:180	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	15	101	theme	follow-up	2577:2585	arg1	months					2567:2572	the last months	2558:2572	the last months of follow-up	2558:2585	The value for LPS was 7.87% (p &lt;0.0001) between 10 days and 1 year, with the majority occurring early in the first 3 months, indicating that the LBP/implant remains steady during the last months of follow-up.
35075504	5	102	theme	technical	918:926	arg1	variations					928:937	its technical variations	914:937	its technical variations (types I-IV)	914:950	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	5	102	theme	technical	918:926	arg1	I-IV					946:949	types I-IV	940:949	types I-IV	940:949	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	18	103	theme	implant	2745:2751	arg1	displacement					2753:2764	minimal implant displacement	2737:2764	minimal implant displacement	2737:2764	There were 2 cases (3%) of minimal implant displacement and no cases of rotation.
35075504	4	104	theme	surgical	718:725	arg1	selection					765:773	a surgical treatment algorithm for RAM technique selection	716:773	a surgical treatment algorithm for RAM technique selection	716:773	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	21	105	theme	accurate	3059:3066	arg1	evaluation					3068:3077	Further accurate evaluation	3051:3077	Further accurate evaluation	3051:3077	Further accurate evaluation is recommended to understand the benefits or disadvantages of CRIMS compared to other RAM techniques.
35075504	22	106	theme	EVIDENCE	3190:3197	arg1	LEVEL					3181:3185	LEVEL	3181:3185	LEVEL OF EVIDENCE IV This journal	3181:3213	LEVEL OF EVIDENCE IV This journal requires that authors assign a level of evidence to each article.
35075504	8	107	theme	mean	1534:1537	arg1	months					1381:1386	at least 6 months	1370:1386	at least 6 months in 5 cases (6.9%)	1370:1404	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	107	theme	mean	1534:1537	arg1	months					1542:1547	mean 34 months	1534:1547	mean 34 months	1534:1547	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	6	108	theme	SM	1031:1032	arg1	pocket					1035:1040	the submuscular (SM) pocket	1014:1040	the submuscular (SM) pocket	1014:1040	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	4	109	theme	RAM	751:753	arg1	selection					765:773	a surgical treatment algorithm for RAM technique selection	716:773	a surgical treatment algorithm for RAM technique selection	716:773	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	0	110	with	Stability	119:127	arg1	Variations					200:209	its Technical Variations	186:209	its Technical Variations	186:209	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	110	with	Stability	119:127	arg1	CRIMS					175:179	CRIMS	175:179	CRIMS	175:179	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	0	110	with	Stability	119:127	arg1	Sling					168:172	Composite Reverse Inferior Muscle Sling	134:172	Composite Reverse Inferior Muscle Sling (CRIMS)	134:180	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	8	111	from	months	1381:1386	arg1	cases					1432:1436	50 cases	1429:1436	50 cases (69.4%)	1429:1444	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	111	from	months	1381:1386	arg1	%					1443:1443	69.4%	1439:1443	69.4%	1439:1443	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	111	from	months	1381:1386	arg1	cases					1393:1397	5 cases	1391:1397	5 cases (6.9%)	1391:1404	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	111	from	months	1381:1386	arg1	%					1403:1403	6.9%	1400:1403	6.9%	1400:1403	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	111	from	months	1381:1386	arg1	cases					1520:1524	7 cases	1518:1524	7 cases	1518:1524	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	4	112	dep	OBJECTIVES	598:607	arg1	defines					708:714	defines	708:714	defines a surgical treatment algorithm for RAM technique selection	708:773	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	4	112	dep	OBJECTIVES	598:607	arg1	revisits					620:627	revisits	620:627	revisits	620:627	OBJECTIVES This study revisits a previously described predictable approach in primary breast augmentation and defines a surgical treatment algorithm for RAM technique selection.
35075504	10	113	theme	lower	1800:1804	arg1	LPS					1820:1822	LPS	1820:1822	LPS	1820:1822	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	10	113	theme	lower	1800:1804	arg1	stretch					1811:1817	lower pole stretch	1800:1817	lower pole stretch (LPS)	1800:1823	Three-dimensional imaging (3DI) obtained from the Divina scanner system was used and followed up for 1 year to evaluate breast position, lower pole stretch (LPS), and intermammary distance (IMD).
35075504	6	114	theme	submuscular	1018:1028	arg1	pocket					1035:1040	the submuscular (SM) pocket	1014:1040	the submuscular (SM) pocket	1014:1040	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	6	115	theme	bridge	1178:1183	arg1	sutures					1185:1191	support points/dermal bridge sutures	1156:1191	support points/dermal bridge sutures	1156:1191	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	22	116	theme	evidence	3255:3262	arg1	level					3246:3250	a level	3244:3250	a level of evidence	3244:3262	LEVEL OF EVIDENCE IV This journal requires that authors assign a level of evidence to each article.
35075504	0	117	theme	Pocket	84:89	arg1	Control					91:97	Pocket Control	84:97	Pocket Control	84:97	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	1	118	theme	complications	320:332	arg1	rates					311:315	the highest rates	299:315	the highest rates of complications and revision	299:345	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	8	119	theme	range	1550:1554	arg1	months					1561:1566	range 6-48 months	1550:1566	range 6-48 months	1550:1566	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	23	120	theme	Medicine	3328:3335	arg1	ratings					3337:3343	these Evidence-Based Medicine ratings	3307:3343	these Evidence-Based Medicine ratings	3307:3343	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
35075504	11	121	theme	Baker	2005:2009	arg1	patients					1922:1929	9 patients	1920:1929	9 patients (12.5%)	1920:1937	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	121	theme	Baker	2005:2009	arg1	contracture					2027:2037	Baker II/III capsular contracture	2005:2037	Baker II/III capsular contracture	2005:2037	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	1	122	theme	revision	338:345	arg1	rates					311:315	the highest rates	299:315	the highest rates of complications and revision	299:345	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	11	123	located	observed	1908:1915	arg1	patients					1922:1929	9 patients	1920:1929	9 patients (12.5%)	1920:1937	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	123	located	observed	1908:1915	arg1	%					2048:2048	1.3%	2045:2048	1.3%	2045:2048	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	123	located	observed	1908:1915	arg1	contracture					2027:2037	Baker II/III capsular contracture	2005:2037	Baker II/III capsular contracture	2005:2037	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	123	located	observed	1908:1915	arg2	cases					1874:1878	Eleven cases	1867:1878	Eleven cases of minor complications	1867:1901	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	123	located	observed	1908:1915	arg1	%					1936:1936	12.5%	1932:1936	12.5%	1932:1936	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	123	located	observed	1908:1915	arg1	1					2042:2042	1	2042:2042	1	2042:2042	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	6	124	theme	gel	997:999	arg1	implant					1001:1007	a silicone gel implant	986:1007	a silicone gel implant	986:1007	CRIMS technique involves placing a silicone gel implant into the submuscular (SM) pocket with an inferior sling of the pectoralis major muscle based on the dimensions of the implant, in combination with support points/dermal bridge sutures to stabilize the implant and glandular tissue at the lower breast pole (LBP).
35075504	20	125	with	stability	3005:3013	arg1	rates					3044:3048	acceptable complication rates	3020:3048	acceptable complication rates	3020:3048	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	17	126	theme	ruptures	2678:2685	arg1	contracture					2697:2707	capsular contracture	2688:2707	capsular contracture	2688:2707	There were no signs of intra/extracapsular ruptures, capsular contracture.
35075504	17	126	theme	ruptures	2678:2685	arg1	signs					2649:2653	no signs	2646:2653	no signs of intra/extracapsular ruptures	2646:2685	There were no signs of intra/extracapsular ruptures, capsular contracture.
35075504	0	127	theme	Smooth	104:109	arg1	Stability					119:127	Smooth Implant Stability	104:127	Smooth Implant Stability	104:127	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	2	128	theme	lateral	391:397	arg1	displacement					399:410	lateral displacement	391:410	lateral displacement	391:410	Complications include implant malposition, lateral displacement, bottoming out, and rotation.
35075504	0	129	theme	Augmentation	12:23	arg1	Mammoplasty					25:35	Reoperative Augmentation Mammoplasty	0:35	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.	0:210	Reoperative Augmentation Mammoplasty: An Algorithm to Optimize Soft-Tissue Support, Pocket Control, and Smooth Implant Stability with Composite Reverse Inferior Muscle Sling (CRIMS) and its Technical Variations.
35075504	12	130	theme	SmoothSilk	2052:2061	arg1	implants					2080:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants were used in all cases, with an average volume decrease of 120 cc.
35075504	5	131	with	variations	928:937	arg1	technique					900:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	composite reverse inferior muscle sling (CRIMS) technique	852:908	METHODS Between 2017 and 2021, 72 patients (144 breasts) underwent RAM with composite reverse inferior muscle sling (CRIMS) technique and its technical variations (types I-IV).
35075504	11	132	theme	capsular	2018:2025	arg1	patients					1922:1929	9 patients	1920:1929	9 patients (12.5%)	1920:1937	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	132	theme	capsular	2018:2025	arg1	contracture					2027:2037	Baker II/III capsular contracture	2005:2037	Baker II/III capsular contracture	2005:2037	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	133	theme	wound	1975:1979	arg1	dehiscence					1981:1990	wound dehiscence	1975:1990	wound dehiscence	1975:1990	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	11	133	theme	wound	1975:1979	arg1	4					1965:1965	4	1965:1965	4	1965:1965	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	1	134	theme	challenging	271:281	arg1	procedure					283:291	a challenging procedure	269:291	a challenging procedure	269:291	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	1	134	theme	challenging	271:281	arg1	mammoplasty					248:258	BACKGROUND Reoperative augmentation mammoplasty	212:258	BACKGROUND Reoperative augmentation mammoplasty (RAM)	212:264	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	14	135	theme	localized	2341:2349	arg1	cysts					2355:2359	localized oil cysts	2341:2359	localized oil cysts	2341:2359	Breast images were performed in a group of 65 patients (90.2%), and in 7 breasts (10.7%), localized oil cysts were observed.
35075504	12	136	theme	silicone	2071:2078	arg1	implants					2080:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants	2052:2087	SmoothSilk surface silicone implants were used in all cases, with an average volume decrease of 120 cc.
35075504	2	137	theme	implant	370:376	arg1	malposition					378:388	implant malposition	370:388	implant malposition	370:388	Complications include implant malposition, lateral displacement, bottoming out, and rotation.
35075504	8	138	from	months	1419:1424	arg1	cases					1432:1436	50 cases	1429:1436	50 cases (69.4%)	1429:1444	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	138	from	months	1419:1424	arg1	%					1443:1443	69.4%	1439:1443	69.4%	1439:1443	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	138	from	months	1419:1424	arg1	cases					1393:1397	5 cases	1391:1397	5 cases (6.9%)	1391:1404	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	138	from	months	1419:1424	arg1	%					1403:1403	6.9%	1400:1403	6.9%	1400:1403	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	138	from	months	1419:1424	arg1	cases					1520:1524	7 cases	1518:1524	7 cases	1518:1524	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	8	139	dep	months	1542:1547	arg1	months					1561:1566	range 6-48 months	1550:1566	range 6-48 months	1550:1566	Patients were followed for at least 6 months in 5 cases (6.9%), at least 12 months in 50 cases (69.4%), for at least 36 months in 10 cases (13.8%), and more than 36 months in 7 cases (9.7%) (mean 34 months; range 6-48 months).
35075504	11	140	theme	complications	1889:1901	arg1	cases					1874:1878	Eleven cases	1867:1878	Eleven cases of minor complications	1867:1901	RESULTS Eleven cases of minor complications were observed in 9 patients (12.5%): hypertrophic scarring in 4 (5.5%), wound dehiscence in 4 (5.5%), Baker II/III capsular contracture in 1 (1.3%).
35075504	13	141	theme	aesthetic	2233:2241	arg1	result					2243:2248	their aesthetic result	2227:2248	their aesthetic result	2227:2248	Sixty-eight patients (94.4%) were either very satisfied/satisfied with their aesthetic result.
35075504	20	142	theme	complication	3031:3042	arg1	rates					3044:3048	acceptable complication rates	3020:3048	acceptable complication rates	3020:3048	An algorithmic approach can facilitate the pre- and intraoperative decision-making process and provide the new pocket control and implant stability with acceptable complication rates.
35075504	23	143	theme	Instructions	3398:3409	arg1	Table					3366:3370	the Table	3362:3370	the Table of Contents or the online Instructions to Authors www.springer.com/00266	3362:3443	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
35075504	21	144	theme	RAM	3165:3167	arg1	techniques					3169:3178	other RAM techniques	3159:3178	other RAM techniques	3159:3178	Further accurate evaluation is recommended to understand the benefits or disadvantages of CRIMS compared to other RAM techniques.
35075504	9	145	theme	resolution	1606:1615	arg1	terms					1597:1601	terms	1597:1601	terms of resolution of symptoms, satisfaction, and complications	1597:1660	Patients were evaluated in terms of resolution of symptoms, satisfaction, and complications.
35075504	3	146	theme	various	482:488	arg1	procedures					490:499	various procedures	482:499	various procedures	482:499	These deformities can be addressed with various procedures, but the pocket control and stability of the new smooth implant surface may present limitations.
35075504	1	147	theme	Reoperative	223:233	arg1	procedure					283:291	a challenging procedure	269:291	a challenging procedure	269:291	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	1	147	theme	Reoperative	223:233	arg1	RAM					261:263	RAM	261:263	RAM	261:263	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	1	147	theme	Reoperative	223:233	arg1	mammoplasty					248:258	BACKGROUND Reoperative augmentation mammoplasty	212:258	BACKGROUND Reoperative augmentation mammoplasty (RAM)	212:264	BACKGROUND Reoperative augmentation mammoplasty (RAM) is a challenging procedure, with the highest rates of complications and revision.
35075504	17	148	theme	capsular	2688:2695	arg1	contracture					2697:2707	capsular contracture	2688:2707	capsular contracture	2688:2707	There were no signs of intra/extracapsular ruptures, capsular contracture.
35075504	17	148	theme	capsular	2688:2695	arg1	signs					2649:2653	no signs	2646:2653	no signs of intra/extracapsular ruptures	2646:2685	There were no signs of intra/extracapsular ruptures, capsular contracture.
35075504	21	149	dep	benefits	3112:3119	arg1	the					3108:3110	the	3108:3110	the	3108:3110	Further accurate evaluation is recommended to understand the benefits or disadvantages of CRIMS compared to other RAM techniques.
35075504	9	150	theme	symptoms	1620:1627	arg1	resolution					1606:1615	resolution	1606:1615	resolution of symptoms, satisfaction, and complications	1606:1660	Patients were evaluated in terms of resolution of symptoms, satisfaction, and complications.
35075504	23	151	theme	Contents	3375:3382	arg1	Table					3366:3370	the Table	3362:3370	the Table of Contents or the online Instructions to Authors www.springer.com/00266	3362:3443	For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .
35075504	16	152	theme	necrosis/seroma	2604:2618	arg1	cases					2591:2595	No cases	2588:2595	No cases of fat necrosis/seroma	2588:2618	No cases of fat necrosis/seroma were observed.
36869488	1	0	theme	punicic	239:245	arg1	acid					247:250	punicic acid	239:250	punicic acid (85%)	239:256	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	1	0	theme	punicic	239:245	arg1	%					255:255	85%	253:255	85%	253:255	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	6	1	dep	82	1186:1187	arg1	ca.					1182:1184	ca.	1182:1184	ca.	1182:1184	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	4	2	theme	tumor	842:846	arg1	factor					857:862	tumor necrosis factor α	842:864	tumor necrosis factor α (TNF-α)	842:872	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	2	theme	tumor	842:846	arg1	TNF-α					867:871	TNF-α	867:871	TNF-α	867:871	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	3	theme	interleukins	810:821	arg1	factor					857:862	tumor necrosis factor α	842:864	tumor necrosis factor α (TNF-α)	842:872	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	3	theme	interleukins	810:821	arg1	TNF-α					867:871	TNF-α	867:871	TNF-α	867:871	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	3	theme	interleukins	810:821	arg1	production					796:805	the production	792:805	the production of interleukins IL-6 and IL-8	792:835	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	3	4	theme	Caco-2	668:673	arg1	cells					675:679	Caco-2 cells	668:679	Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS)	668:741	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	10	5	theme	present	1617:1623	arg1	work					1625:1628	The present work	1613:1628	The present work	1613:1628	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	2	6	theme	digestion	520:528	arg1	model					530:534	a static gastrointestinal in vitro digestion model	485:534	a static gastrointestinal in vitro digestion model	485:534	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	3	7	theme	mediator	709:716	arg1	LPS					738:740	LPS	738:740	LPS	738:740	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	3	7	theme	mediator	709:716	arg1	lipopolysaccharide					718:735	the inflammatory mediator lipopolysaccharide	692:735	the inflammatory mediator lipopolysaccharide (LPS)	692:741	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	2	8	theme	gastrointestinal	494:509	arg1	model					530:534	a static gastrointestinal in vitro digestion model	485:534	a static gastrointestinal in vitro digestion model	485:534	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	0	9	theme	bioaccessible	111:123	arg1	fraction					125:132	the bioaccessible fraction	107:132	the bioaccessible fraction from in vitro gastrointestinal digestion	107:173	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	0	10	from	technologies	72:83	arg1	cells					95:99	Caco-2 cells	88:99	Caco-2 cells	88:99	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	7	11	theme	adequate	1280:1287	arg1	size					1298:1301	adequate particle size	1280:1301	adequate particle size	1280:1301	Micellar phases of EPO and SCPO showed high stability and adequate particle size.
36869488	3	12	dep	in	622:623	arg1	vitro					625:629	vitro	625:629	vitro	625:629	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	5	13	theme	micellar	1015:1022	arg1	%					1037:1037	93%	1035:1037	93%	1035:1037	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	13	theme	micellar	1015:1022	arg1	phase					1024:1028	micellar phase	1015:1028	micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components	1015:1102	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	6	14	theme	CO2	1152:1154	arg1	SCPO					1173:1176	SCPO	1173:1176	SCPO	1173:1176	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	6	14	theme	CO2	1152:1154	arg1	oil					1168:1170	supercritical CO2 pomegranate oil	1138:1170	supercritical CO2 pomegranate oil (SCPO)	1138:1177	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	10	15	theme	anti-inflammatory	1684:1700	arg1	response					1702:1709	anti-inflammatory response	1684:1709	anti-inflammatory response	1684:1709	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	8	16	theme	IL-6	1372:1375	arg1	production					1358:1367	the production	1354:1367	the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells	1354:1422	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	6	17	theme	similar	1195:1201	arg1	composition					1209:1219	similar lipid composition	1195:1219	similar lipid composition	1195:1219	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	8	18	dep	integrity	1443:1451	arg1	measured					1478:1485	measured	1478:1485	measured by transepithelial electrical resistance (TEER)	1478:1533	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	10	19	theme	SCPO	1727:1730	arg1	bioaccessibility					1663:1678	bioaccessibility	1663:1678	bioaccessibility	1663:1678	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	10	19	theme	SCPO	1727:1730	arg1	response					1702:1709	anti-inflammatory response	1684:1709	anti-inflammatory response	1684:1709	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	10	19	theme	SCPO	1727:1730	arg1	digestibility					1648:1660	good digestibility	1643:1660	good digestibility	1643:1660	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	8	20	theme	IL-8	1378:1381	arg1	production					1358:1367	the production	1354:1367	the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells	1354:1422	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	8	21	from	production	1358:1367	arg1	cells					1418:1422	LPS stimulated caco-2 cells	1396:1422	LPS stimulated caco-2 cells	1396:1422	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	1	22	theme	several	284:290	arg1	activities					303:312	several biological activities	284:312	several biological activities	284:312	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	10	23	theme	EPO	1719:1721	arg1	bioaccessibility					1663:1678	bioaccessibility	1663:1678	bioaccessibility	1663:1678	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	10	23	theme	EPO	1719:1721	arg1	response					1702:1709	anti-inflammatory response	1684:1709	anti-inflammatory response	1684:1709	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	10	23	theme	EPO	1719:1721	arg1	digestibility					1648:1660	good digestibility	1643:1660	good digestibility	1643:1660	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	2	24	theme	supercritical	432:444	arg1	technologies					450:461	supercritical CO2 technologies	432:461	supercritical CO2 technologies	432:461	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	8	25	theme	TNF-α	1387:1391	arg1	production					1358:1367	the production	1354:1367	the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells	1354:1422	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	5	26	theme	free	1045:1048	arg1	acids					1056:1060	free fatty acids	1045:1060	free fatty acids	1045:1060	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	9	27	from	evident	1595:1601	arg1	case					1543:1546	the case	1539:1546	the case of SCPO	1539:1554	In the case of SCPO, the anti-inflammatory effect was only evident for IL-8.
36869488	0	28	theme	Caco-2	88:93	arg1	cells					95:99	Caco-2 cells	88:99	Caco-2 cells	88:99	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	4	29	theme	monolayer	897:905	arg1	integrity					907:915	the monolayer integrity	893:915	the monolayer integrity	893:915	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	8	30	theme	caco-2	1411:1416	arg1	cells					1418:1422	LPS stimulated caco-2 cells	1396:1422	LPS stimulated caco-2 cells	1396:1422	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	7	31	theme	EPO	1241:1243	arg1	phases					1231:1236	Micellar phases	1222:1236	Micellar phases of EPO and SCPO	1222:1252	Micellar phases of EPO and SCPO showed high stability and adequate particle size.
36869488	8	32	theme	electrical	1506:1515	arg1	TEER					1529:1532	TEER	1529:1532	TEER	1529:1532	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	8	32	theme	electrical	1506:1515	arg1	resistance					1517:1526	transepithelial electrical resistance	1490:1526	transepithelial electrical resistance (TEER)	1490:1533	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	2	33	theme	pomegranate	333:343	arg1	oils					345:348	two pomegranate oils	329:348	two pomegranate oils	329:348	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	0	34	theme	Anti-inflammatory	0:16	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of two pomegranate seed oils	0:52	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	5	35	theme	major	1087:1091	arg1	components					1093:1102	major components	1087:1102	major components	1087:1102	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	9	36	theme	SCPO	1551:1554	arg1	case					1543:1546	the case	1539:1546	the case of SCPO	1539:1554	In the case of SCPO, the anti-inflammatory effect was only evident for IL-8.
36869488	5	37	with	phase	1024:1028	arg1	monoacylglycerols					1066:1082	monoacylglycerols	1066:1082	monoacylglycerols as major components	1066:1102	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	37	with	phase	1024:1028	arg1	acids					1056:1060	free fatty acids	1045:1060	free fatty acids	1045:1060	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	2	38	theme	sequential	373:382	arg1	extraction					384:393	a two-step sequential extraction	362:393	a two-step sequential extraction	362:393	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	0	39	dep	in	139:140	arg1	vitro					142:146	vitro	142:146	vitro	142:146	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	1	40	contain	contain	194:200	arg2	oil					212:214	up to 20% oil	202:214	up to 20% oil	202:214	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	1	40	contain	contain	194:200	arg1	seeds					188:192	Pomegranate seeds	176:192	Pomegranate seeds	176:192	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	1	41	theme	Pomegranate	176:186	arg1	seeds					188:192	Pomegranate seeds	176:192	Pomegranate seeds	176:192	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	0	42	theme	pomegranate	32:42	arg1	oils					49:52	two pomegranate seed oils	28:52	two pomegranate seed oils	28:52	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	8	43	theme	cell	1460:1463	arg1	monolayer					1465:1473	the cell monolayer	1456:1473	the cell monolayer	1456:1473	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	0	44	theme	oils	49:52	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of two pomegranate seed oils	0:52	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	6	45	theme	micellar	1109:1116	arg1	%					1188:1188	ca. 82%	1182:1188	ca. 82% with similar lipid composition	1182:1219	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	6	45	theme	micellar	1109:1116	arg1	phase					1118:1122	The micellar phase	1105:1122	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO)	1105:1177	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	8	46	theme	anti-inflammatory	1317:1333	arg1	response					1335:1342	an anti-inflammatory response	1314:1342	an anti-inflammatory response	1314:1342	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	3	47	theme	in	622:623	arg1	model					631:635	an in vitro model	619:635	an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS)	619:741	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	6	48	with	%	1188:1188	arg1	composition					1209:1219	similar lipid composition	1195:1219	similar lipid composition	1195:1219	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	1	49	theme	high	223:226	arg1	content					228:234	a high content	221:234	a high content of punicic acid (85%), which is responsible for several biological activities	221:312	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	2	50	dep	in	511:512	arg1	vitro					514:518	vitro	514:518	vitro	514:518	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	5	51	theme	expeller	953:960	arg1	oil					974:976	expeller pomegranate oil	953:976	expeller pomegranate oil (EPO)	953:982	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	51	theme	expeller	953:960	arg1	EPO					979:981	EPO	979:981	EPO	979:981	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	52	dep	%	1037:1037	arg1	ca.					1031:1033	ca.	1031:1033	ca.	1031:1033	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	0	53	from	digestion	165:173	arg1	fraction					125:132	the bioaccessible fraction	107:132	the bioaccessible fraction from in vitro gastrointestinal digestion	107:173	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	3	54	theme	intestinal	640:649	arg1	inflammation					651:662	intestinal inflammation	640:662	intestinal inflammation	640:662	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	10	55	theme	good	1643:1646	arg1	digestibility					1648:1660	good digestibility	1643:1660	good digestibility	1643:1660	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	1	56	theme	acid	247:250	arg1	content					228:234	a high content	221:234	a high content of punicic acid (85%), which is responsible for several biological activities	221:312	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	4	57	dep	assessed	770:777	arg1	measuring					782:790	measuring	782:790	measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α)	782:872	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	57	dep	assessed	770:777	arg1	evaluating					882:891	evaluating	882:891	evaluating the monolayer integrity	882:915	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	10	58	dep	EPO	1719:1721	arg1	oils					1732:1735	oils	1732:1735	oils	1732:1735	The present work demonstrates good digestibility, bioaccessibility and anti-inflammatory response of both EPO and SCPO oils.
36869488	3	59	theme	cells	675:679	arg1	model					631:635	an in vitro model	619:635	an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS)	619:741	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	9	60	theme	anti-inflammatory	1561:1577	arg1	effect					1579:1584	the anti-inflammatory effect	1557:1584	the anti-inflammatory effect	1557:1584	In the case of SCPO, the anti-inflammatory effect was only evident for IL-8.
36869488	9	60	theme	anti-inflammatory	1561:1577	arg1	evident					1595:1601	evident	1595:1601	evident	1595:1601	In the case of SCPO, the anti-inflammatory effect was only evident for IL-8.
36869488	2	61	theme	static	487:492	arg1	model					530:534	a static gastrointestinal in vitro digestion model	485:534	a static gastrointestinal in vitro digestion model	485:534	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	3	62	theme	inflammatory	696:707	arg1	LPS					738:740	LPS	738:740	LPS	738:740	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	3	62	theme	inflammatory	696:707	arg1	lipopolysaccharide					718:735	the inflammatory mediator lipopolysaccharide	692:735	the inflammatory mediator lipopolysaccharide (LPS)	692:741	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	0	63	theme	in	139:140	arg1	digestion					165:173	in vitro gastrointestinal digestion	139:173	in vitro gastrointestinal digestion	139:173	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	9	64	from	case	1543:1546	arg1	effect					1579:1584	the anti-inflammatory effect	1557:1584	the anti-inflammatory effect	1557:1584	In the case of SCPO, the anti-inflammatory effect was only evident for IL-8.
36869488	9	64	from	case	1543:1546	arg1	evident					1595:1601	evident	1595:1601	evident	1595:1601	In the case of SCPO, the anti-inflammatory effect was only evident for IL-8.
36869488	6	65	theme	pomegranate	1156:1166	arg1	SCPO					1173:1176	SCPO	1173:1176	SCPO	1173:1176	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	6	65	theme	pomegranate	1156:1166	arg1	oil					1168:1170	supercritical CO2 pomegranate oil	1138:1170	supercritical CO2 pomegranate oil (SCPO)	1138:1177	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	0	66	theme	gastrointestinal	148:163	arg1	digestion					165:173	in vitro gastrointestinal digestion	139:173	in vitro gastrointestinal digestion	139:173	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	5	67	theme	phase	1024:1028	arg1	%					1037:1037	93%	1035:1037	93%	1035:1037	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	67	theme	phase	1024:1028	arg1	amount					1005:1010	the highest amount	993:1010	the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components	993:1102	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	67	theme	phase	1024:1028	arg1	phase					1024:1028	micellar phase	1015:1028	micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components	1015:1102	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	68	theme	fatty	1050:1054	arg1	acids					1056:1060	free fatty acids	1045:1060	free fatty acids	1045:1060	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	4	69	theme	necrosis	848:855	arg1	factor					857:862	tumor necrosis factor α	842:864	tumor necrosis factor α (TNF-α)	842:872	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	69	theme	necrosis	848:855	arg1	TNF-α					867:871	TNF-α	867:871	TNF-α	867:871	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	6	70	theme	supercritical	1138:1150	arg1	SCPO					1173:1176	SCPO	1173:1176	SCPO	1173:1176	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	6	70	theme	supercritical	1138:1150	arg1	oil					1168:1170	supercritical CO2 pomegranate oil	1138:1170	supercritical CO2 pomegranate oil (SCPO)	1138:1177	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	6	71	theme	lipid	1203:1207	arg1	composition					1209:1219	similar lipid composition	1195:1219	similar lipid composition	1195:1219	The micellar phase obtained with supercritical CO2 pomegranate oil (SCPO) is ca. 82% with similar lipid composition.
36869488	1	72	dep	%	210:210	arg1	20					208:209	20	208:209	20	208:209	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	3	73	theme	micellar	576:583	arg1	phases					585:590	The micellar phases	572:590	The micellar phases obtained	572:599	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	2	74	theme	in	511:512	arg1	model					530:534	a static gastrointestinal in vitro digestion model	485:534	a static gastrointestinal in vitro digestion model	485:534	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	7	75	theme	high	1261:1264	arg1	stability					1266:1274	high stability	1261:1274	high stability	1261:1274	Micellar phases of EPO and SCPO showed high stability and adequate particle size.
36869488	0	76	theme	green	66:70	arg1	technologies					72:83	green technologies	66:83	green technologies in Caco-2 cells	66:99	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	5	77	theme	highest	997:1003	arg1	%					1037:1037	93%	1035:1037	93%	1035:1037	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	77	theme	highest	997:1003	arg1	amount					1005:1010	the highest amount	993:1010	the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components	993:1102	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	77	theme	highest	997:1003	arg1	phase					1024:1028	micellar phase	1015:1028	micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components	1015:1102	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	8	78	dep	shows	1308:1312	arg1	reducing					1345:1352	reducing	1345:1352	reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells	1345:1422	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	8	78	dep	shows	1308:1312	arg1	increasing					1428:1437	increasing	1428:1437	increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER)	1428:1533	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	2	79	theme	CO2	446:448	arg1	technologies					450:461	supercritical CO2 technologies	432:461	supercritical CO2 technologies	432:461	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	7	80	theme	SCPO	1249:1252	arg1	phases					1231:1236	Micellar phases	1222:1236	Micellar phases of EPO and SCPO	1222:1252	Micellar phases of EPO and SCPO showed high stability and adequate particle size.
36869488	1	81	theme	biological	292:301	arg1	activities					303:312	several biological activities	284:312	several biological activities	284:312	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	8	82	theme	stimulated	1400:1409	arg1	cells					1418:1422	LPS stimulated caco-2 cells	1396:1422	LPS stimulated caco-2 cells	1396:1422	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	1	83	dep	oil	212:214	arg1	%					210:210	%	210:210	%	210:210	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
36869488	7	84	theme	Micellar	1222:1229	arg1	phases					1231:1236	Micellar phases	1222:1236	Micellar phases of EPO and SCPO	1222:1252	Micellar phases of EPO and SCPO showed high stability and adequate particle size.
36869488	4	85	dep	interleukins	810:821	arg1	IL-6					823:826	IL-6	823:826	IL-6	823:826	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	85	dep	interleukins	810:821	arg1	interleukins					810:821	interleukins IL-6 and IL-8	810:835	interleukins IL-6 and IL-8	810:835	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	4	85	dep	interleukins	810:821	arg1	IL-8					832:835	IL-8	832:835	IL-8	832:835	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	2	86	theme	two-step	364:371	arg1	extraction					384:393	a two-step sequential extraction	362:393	a two-step sequential extraction	362:393	In this work, two pomegranate oils obtained by a two-step sequential extraction, first with an expeller and then via supercritical CO2 technologies, have been studied in a static gastrointestinal in vitro digestion model to evaluate their bioaccessibility.
36869488	0	87	theme	seed	44:47	arg1	oils					49:52	two pomegranate seed oils	28:52	two pomegranate seed oils	28:52	Anti-inflammatory effect of two pomegranate seed oils obtained by green technologies in Caco-2 cells using the bioaccessible fraction from in vitro gastrointestinal digestion.
36869488	5	88	theme	pomegranate	962:972	arg1	oil					974:976	expeller pomegranate oil	953:976	expeller pomegranate oil (EPO)	953:982	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	5	88	theme	pomegranate	962:972	arg1	EPO					979:981	EPO	979:981	EPO	979:981	The results obtained indicate that expeller pomegranate oil (EPO) provides the highest amount of micellar phase (ca. 93%) with free fatty acids and monoacylglycerols as major components.
36869488	8	89	theme	monolayer	1465:1473	arg1	integrity					1443:1451	the integrity	1439:1451	the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER)	1439:1533	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	7	90	theme	particle	1289:1296	arg1	size					1298:1301	adequate particle size	1280:1301	adequate particle size	1280:1301	Micellar phases of EPO and SCPO showed high stability and adequate particle size.
36869488	8	91	theme	transepithelial	1490:1504	arg1	TEER					1529:1532	TEER	1529:1532	TEER	1529:1532	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	8	91	theme	transepithelial	1490:1504	arg1	resistance					1517:1526	transepithelial electrical resistance	1490:1526	transepithelial electrical resistance (TEER)	1490:1533	EPO shows an anti-inflammatory response, reducing the production of IL-6, IL-8 and TNF-α in LPS stimulated caco-2 cells and increasing the integrity of the cell monolayer as measured by transepithelial electrical resistance (TEER).
36869488	4	92	theme	Inflammatory	744:755	arg1	response					757:764	Inflammatory response	744:764	Inflammatory response	744:764	Inflammatory response was assessed by measuring the production of interleukins IL-6 and IL-8, and tumor necrosis factor α (TNF-α), and by evaluating the monolayer integrity.
36869488	3	93	theme	inflammation	651:662	arg1	model					631:635	an in vitro model	619:635	an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS)	619:741	The micellar phases obtained were evaluated by an in vitro model of intestinal inflammation and Caco-2 cells exposed to the inflammatory mediator lipopolysaccharide (LPS).
36869488	1	94	dep	20	208:209	arg1	to					205:206	to	205:206	to	205:206	Pomegranate seeds contain up to 20% oil with a high content of punicic acid (85%), which is responsible for several biological activities.
35700436	7	0	theme	uninjured	1303:1311	arg1	controls					1313:1320	ACLR-T1ρ Low and uninjured controls	1286:1320	ACLR-T1ρ Low and uninjured controls 6 months post-ACLR	1286:1339	RESULTS ACLR-T1ρ High exhibited lesser KAM than ACLR-T1ρ Low and uninjured controls 6 months post-ACLR.
35700436	10	1	theme	lesser	1701:1706	arg1	KFM					1708:1710	lesser KFM	1701:1710	lesser KFM	1701:1710	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	3	2	theme	low	692:694	arg1	profiles					724:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	1	3	theme	cruciate	306:313	arg1	ACLR					340:343	ACLR	340:343	ACLR	340:343	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	1	3	theme	cruciate	306:313	arg1	reconstruction					324:337	anterior cruciate ligament reconstruction	297:337	anterior cruciate ligament reconstruction (ACLR)	297:344	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	7	4	theme	Low	1295:1297	arg1	controls					1313:1320	ACLR-T1ρ Low and uninjured controls	1286:1320	ACLR-T1ρ Low and uninjured controls 6 months post-ACLR	1286:1339	RESULTS ACLR-T1ρ High exhibited lesser KAM than ACLR-T1ρ Low and uninjured controls 6 months post-ACLR.
35700436	4	5	theme	mass	858:861	arg1	index					863:867	body mass index	853:867	body mass index	853:867	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	6	theme	mass	956:959	arg1	index					961:965	body mass index	951:965	body mass index	951:965	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	3	7	theme	uninjured	737:745	arg1	controls					747:754	uninjured controls	737:754	uninjured controls	737:754	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	4	8	dep	females	926:932	arg1	age					935:937	age	935:937	age	935:937	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	8	dep	females	926:932	arg1	kg·m					979:982	24.2 ± 3.5 kg·m -2	968:985	24.2 ± 3.5 kg·m -2	968:985	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	8	dep	females	926:932	arg1	index					961:965	body mass index	951:965	body mass index	951:965	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	8	dep	females	926:932	arg1	yr					947:948	22 ± 4 yr	940:948	22 ± 4 yr	940:948	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	3	9	with	ACLR	670:673	arg1	profiles					724:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	3	9	with	ACLR	670:673	arg1	controls					747:754	uninjured controls	737:754	uninjured controls	737:754	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	9	10	theme	Low	1485:1487	arg1	ACLR-T1ρ					1476:1483	ACLR-T1ρ Low	1476:1487	ACLR-T1ρ Low between 6 and 12 months	1476:1511	KAM increased in ACLR-T1ρ High and decreased in ACLR-T1ρ Low between 6 and 12 months, both groups becoming more similar to uninjured controls.
35700436	4	11	theme	6	992:992	arg1	months					1001:1006	6 and 12 months post-ACLR	992:1016	months	1001:1006	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	3	12	theme	study	572:576	arg1	purpose					556:562	The purpose	552:562	The purpose of this study	552:576	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	7	13	theme	lesser	1270:1275	arg1	KAM					1277:1279	lesser KAM	1270:1279	lesser KAM	1270:1279	RESULTS ACLR-T1ρ High exhibited lesser KAM than ACLR-T1ρ Low and uninjured controls 6 months post-ACLR.
35700436	9	14	theme	6	1497:1497	arg1	months					1506:1511	6 and 12 months	1497:1511	months	1506:1511	KAM increased in ACLR-T1ρ High and decreased in ACLR-T1ρ Low between 6 and 12 months, both groups becoming more similar to uninjured controls.
35700436	4	15	theme	12	998:999	arg1	months					1001:1006	6 and 12 months post-ACLR	992:1016	months	1001:1006	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	10	16	theme	scant	1582:1586	arg1	differences					1588:1598	scant differences	1582:1598	scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR	1582:1669	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	9	17	theme	12	1503:1504	arg1	months					1506:1511	6 and 12 months	1497:1511	months	1506:1511	KAM increased in ACLR-T1ρ High and decreased in ACLR-T1ρ Low between 6 and 12 months, both groups becoming more similar to uninjured controls.
35700436	3	18	theme	external	593:600	arg1	KAM					618:620	KAM	618:620	KAM	618:620	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	3	18	theme	external	593:600	arg1	adduction					607:615	external knee adduction	593:615	external knee adduction (KAM)	593:621	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	4	19	dep	collected	788:796	arg1	females					926:932	50% females	922:932	50% females	922:932	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	10	20	dep	ACLR-T1ρ	1615:1622	arg1	months					1654:1659	6 or 12 months	1646:1659	High and ACLR-T1ρ Low 6 or 12 months	1624:1659	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	11	21	theme	CONCLUSIONS	1746:1756	arg1	Associations					1758:1769	CONCLUSIONS Associations	1746:1769	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM	1746:1817	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	1	22	theme	cartilage	139:147	arg1	resonance					162:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance	113:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times	113:195	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	4	23	theme	±	875:875	arg1	kg·m					881:884	23.9 ± 2.8 kg·m -2	870:887	23.9 ± 2.8 kg·m -2	870:887	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	10	24	theme	ACLR-T1ρ	1633:1640	arg1	months					1654:1659	6 or 12 months	1646:1659	High and ACLR-T1ρ Low 6 or 12 months	1624:1659	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	1	25	theme	magnetic	153:160	arg1	resonance					162:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance	113:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times	113:195	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	11	26	theme	KAM	1911:1913	arg1	post-ACLR					1924:1932	insufficient KAM 6 months post-ACLR	1898:1932	insufficient KAM 6 months post-ACLR	1898:1932	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	1	27	theme	T1ρ	149:151	arg1	resonance					162:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance	113:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times	113:195	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	2	28	theme	post-ACLR	541:549	arg1	times					525:529	greater T1ρ relaxation times	502:529	greater T1ρ relaxation times 12 months post-ACLR	502:549	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	4	29	theme	post-ACLR	1008:1016	arg1	months					1001:1006	6 and 12 months post-ACLR	992:1016	months	1001:1006	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	5	30	theme	post-ACLR	1107:1115	arg1	times					1132:1136	12-month post-ACLR T1ρ relaxation times	1098:1136	12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis	1098:1169	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	5	31	theme	ACLR-T1ρ	1046:1053	arg1	groups					1069:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups	1019:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups	1019:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	9	32	dep	decreased	1463:1471	arg1	becoming					1526:1533	becoming	1526:1533	becoming more similar to uninjured controls	1526:1568	KAM increased in ACLR-T1ρ High and decreased in ACLR-T1ρ Low between 6 and 12 months, both groups becoming more similar to uninjured controls.
35700436	2	33	from	changes	356:362	arg1	times					382:386	T1ρ relaxation times	367:386	T1ρ relaxation times	367:386	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	4	34	theme	%	924:924	arg1	females					926:932	50% females	922:932	50% females	922:932	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	11	35	from	normalization	1840:1852	arg1	individuals					1864:1874	individuals	1864:1874	individuals who initially exhibit insufficient KAM 6 months post-ACLR	1864:1932	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	6	36	theme	variance	1195:1202	arg1	analyses					1183:1190	Functional analyses	1172:1190	Functional analyses of variance	1172:1202	Functional analyses of variance were used to compare KAM and KFM.
35700436	3	37	dep	adduction	607:615	arg1	moments					641:647	moments	641:647	moments	641:647	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	10	38	from	differences	1588:1598	arg1	KFM					1603:1605	KFM	1603:1605	KFM	1603:1605	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	5	39	dep	High	1028:1031	arg1	n					1035:1035	n = 9	1035:1039	n = 9	1035:1039	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	5	39	dep	High	1028:1031	arg1	Low					1055:1057	Low	1055:1057	Low	1055:1057	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	5	39	dep	High	1028:1031	arg1	n					1061:1061	n = 17	1061:1066	n = 17	1061:1066	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	5	40	theme	k-means	1146:1152	arg1	analysis					1162:1169	a k-means cluster analysis	1144:1169	a k-means cluster analysis	1144:1169	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	4	41	theme	±	943:943	arg1	yr					947:948	22 ± 4 yr	940:948	22 ± 4 yr	940:948	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	0	42	theme	Worse	0:4	arg1	Composition					29:39	Worse Tibiofemoral Cartilage Composition	0:39	Worse Tibiofemoral Cartilage Composition	0:39	Worse Tibiofemoral Cartilage Composition Is Associated with Insufficient Gait Kinetics After ACL Reconstruction.
35700436	8	43	theme	greater	1365:1371	arg1	KAM					1373:1375	greater KAM	1365:1375	greater KAM	1365:1375	ACLR-T1ρ Low exhibited greater KAM than uninjured controls 6 and 12 months post-ACLR.
35700436	5	44	theme	relaxation	1121:1130	arg1	times					1132:1136	12-month post-ACLR T1ρ relaxation times	1098:1136	12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis	1098:1169	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	2	45	theme	knee	470:473	arg1	loading					481:487	excessive or insufficient knee joint loading	444:487	excessive or insufficient knee joint loading	444:487	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	0	46	theme	Cartilage	19:27	arg1	Composition					29:39	Worse Tibiofemoral Cartilage Composition	0:39	Worse Tibiofemoral Cartilage Composition	0:39	Worse Tibiofemoral Cartilage Composition Is Associated with Insufficient Gait Kinetics After ACL Reconstruction.
35700436	5	47	theme	=	1037:1037	arg1	n					1035:1035	n = 9	1035:1039	n = 9	1035:1039	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	1	48	theme	Greater	121:127	arg1	resonance					162:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance	113:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times	113:195	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	2	49	theme	relaxation	514:523	arg1	times					525:529	greater T1ρ relaxation times	502:529	greater T1ρ relaxation times 12 months post-ACLR	502:549	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	2	50	theme	greater	502:508	arg1	times					525:529	greater T1ρ relaxation times	502:529	greater T1ρ relaxation times 12 months post-ACLR	502:549	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	0	51	theme	Gait	73:76	arg1	Kinetics					78:85	Insufficient Gait Kinetics	60:85	Insufficient Gait Kinetics After ACL Reconstruction	60:110	Worse Tibiofemoral Cartilage Composition Is Associated with Insufficient Gait Kinetics After ACL Reconstruction.
35700436	5	52	theme	ACLR-T1ρ	1019:1026	arg1	groups					1069:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups	1019:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups	1019:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	10	53	theme	High	1624:1627	arg1	months					1654:1659	6 or 12 months	1646:1659	High and ACLR-T1ρ Low 6 or 12 months	1624:1659	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	1	54	theme	posttraumatic	250:262	arg1	development					279:289	posttraumatic osteoarthritis development	250:289	posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR)	250:344	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	1	55	attach	linked	240:245	arg1	development					279:289	posttraumatic osteoarthritis development	250:289	posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR)	250:344	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	1	55	attach	linked	240:245	arg2	resonance					162:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance	113:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times	113:195	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	5	56	theme	=	1063:1063	arg1	n					1061:1061	n = 17	1061:1066	n = 17	1061:1066	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	9	57	theme	High	1454:1457	arg1	ACLR-T1ρ					1445:1452	ACLR-T1ρ High	1445:1457	ACLR-T1ρ High	1445:1457	KAM increased in ACLR-T1ρ High and decreased in ACLR-T1ρ Low between 6 and 12 months, both groups becoming more similar to uninjured controls.
35700436	4	58	theme	body	951:954	arg1	index					961:965	body mass index	951:965	body mass index	951:965	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	3	59	theme	relaxation	713:722	arg1	profiles					724:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	2	60	theme	gait	408:411	arg1	biomechanics					413:424	gait biomechanics	408:424	gait biomechanics	408:424	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	1	61	theme	anterior	297:304	arg1	ACLR					340:343	ACLR	340:343	ACLR	340:343	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	1	61	theme	anterior	297:304	arg1	reconstruction					324:337	anterior cruciate ligament reconstruction	297:337	anterior cruciate ligament reconstruction (ACLR)	297:344	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	7	62	theme	ACLR-T1ρ	1286:1293	arg1	controls					1313:1320	ACLR-T1ρ Low and uninjured controls	1286:1320	ACLR-T1ρ Low and uninjured controls 6 months post-ACLR	1286:1339	RESULTS ACLR-T1ρ High exhibited lesser KAM than ACLR-T1ρ Low and uninjured controls 6 months post-ACLR.
35700436	11	63	theme	months	1917:1922	arg1	post-ACLR					1924:1932	insufficient KAM 6 months post-ACLR	1898:1932	insufficient KAM 6 months post-ACLR	1898:1932	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	3	64	theme	tibiofemoral	696:707	arg1	profiles					724:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	1	65	theme	ligament	315:322	arg1	ACLR					340:343	ACLR	340:343	ACLR	340:343	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	1	65	theme	ligament	315:322	arg1	reconstruction					324:337	anterior cruciate ligament reconstruction	297:337	anterior cruciate ligament reconstruction (ACLR)	297:344	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	2	66	theme	T1ρ	367:369	arg1	times					382:386	T1ρ relaxation times	367:386	T1ρ relaxation times	367:386	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	4	67	theme	body	853:856	arg1	index					863:867	body mass index	853:867	body mass index	853:867	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	7	68	theme	High	1255:1258	arg1	RESULTS					1238:1244	RESULTS	1238:1244	RESULTS ACLR-T1ρ High	1238:1258	RESULTS ACLR-T1ρ High exhibited lesser KAM than ACLR-T1ρ Low and uninjured controls 6 months post-ACLR.
35700436	10	69	theme	uninjured	1726:1734	arg1	controls					1736:1743	uninjured controls	1726:1743	uninjured controls	1726:1743	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	11	70	theme	T1ρ	1785:1787	arg1	profiles					1789:1796	worse T1ρ profiles	1779:1796	worse T1ρ profiles	1779:1796	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	6	71	used	used	1209:1212	arg2	analyses					1183:1190	Functional analyses	1172:1190	Functional analyses of variance	1172:1202	Functional analyses of variance were used to compare KAM and KFM.
35700436	11	72	from	profiles	1789:1796	arg1	KAM					1815:1817	KAM	1815:1817	KAM	1815:1817	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	10	73	dep	High	1624:1627	arg1	Low					1642:1644	Low	1642:1644	Low	1642:1644	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	3	74	theme	knee	602:605	arg1	KAM					618:620	KAM	618:620	KAM	618:620	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	3	74	theme	knee	602:605	arg1	adduction					607:615	external knee adduction	593:615	external knee adduction (KAM)	593:621	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	1	75	theme	proteoglycan	211:222	arg1	density					224:230	less proteoglycan density	206:230	less proteoglycan density	206:230	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	10	76	theme	ACLR-T1ρ	1615:1622	arg1	post-ACLR					1661:1669	ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR	1615:1669	ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR	1615:1669	There were scant differences in KFM between ACLR-T1ρ High and ACLR-T1ρ Low 6 or 12 months post-ACLR, but both groups demonstrated lesser KFM compared with uninjured controls.
35700436	4	77	dep	females	828:834	arg1	kg·m					881:884	23.9 ± 2.8 kg·m -2	870:887	23.9 ± 2.8 kg·m -2	870:887	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	77	dep	females	828:834	arg1	index					863:867	body mass index	853:867	body mass index	853:867	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	77	dep	females	828:834	arg1	yr					849:850	22 ± 4 yr	842:850	22 ± 4 yr	842:850	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	77	dep	females	828:834	arg1	age					837:839	age	837:839	age	837:839	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	1	78	theme	articular	129:137	arg1	resonance					162:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance	113:170	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times	113:195	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	11	79	theme	KAM	1857:1859	arg1	normalization					1840:1852	the normalization	1836:1852	the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR	1836:1932	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	4	80	dep	METHODS	757:763	arg1	biomechanics					770:781	Gait biomechanics	765:781	METHODS Gait biomechanics	757:781	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	5	81	theme	12-month	1098:1105	arg1	times					1132:1136	12-month post-ACLR T1ρ relaxation times	1098:1136	12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis	1098:1169	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	11	82	theme	worse	1779:1783	arg1	profiles					1789:1796	worse T1ρ profiles	1779:1796	worse T1ρ profiles	1779:1796	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	9	83	theme	uninjured	1551:1559	arg1	controls					1561:1568	uninjured controls	1551:1568	uninjured controls	1551:1568	KAM increased in ACLR-T1ρ High and decreased in ACLR-T1ρ Low between 6 and 12 months, both groups becoming more similar to uninjured controls.
35700436	0	84	theme	ACL	93:95	arg1	Reconstruction					97:110	ACL Reconstruction	93:110	ACL Reconstruction	93:110	Worse Tibiofemoral Cartilage Composition Is Associated with Insufficient Gait Kinetics After ACL Reconstruction.
35700436	1	85	theme	relaxation	180:189	arg1	times					191:195	relaxation times	180:195	relaxation times	180:195	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	6	86	theme	Functional	1172:1181	arg1	analyses					1183:1190	Functional analyses	1172:1190	Functional analyses of variance	1172:1202	Functional analyses of variance were used to compare KAM and KFM.
35700436	5	87	theme	cluster	1154:1160	arg1	analysis					1162:1169	a k-means cluster analysis	1144:1169	a k-means cluster analysis	1144:1169	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	0	88	theme	Tibiofemoral	6:17	arg1	Composition					29:39	Worse Tibiofemoral Cartilage Composition	0:39	Worse Tibiofemoral Cartilage Composition	0:39	Worse Tibiofemoral Cartilage Composition Is Associated with Insufficient Gait Kinetics After ACL Reconstruction.
35700436	2	89	attach	linked	492:497	arg1	times					525:529	greater T1ρ relaxation times	502:529	greater T1ρ relaxation times 12 months post-ACLR	502:549	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	2	89	attach	linked	492:497	arg2	loading					481:487	excessive or insufficient knee joint loading	444:487	excessive or insufficient knee joint loading	444:487	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	5	90	theme	T1ρ	1117:1119	arg1	times					1132:1136	12-month post-ACLR T1ρ relaxation times	1098:1136	12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis	1098:1169	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	2	91	theme	joint	475:479	arg1	loading					481:487	excessive or insufficient knee joint loading	444:487	excessive or insufficient knee joint loading	444:487	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	8	92	theme	uninjured	1382:1390	arg1	controls					1392:1399	uninjured controls	1382:1399	uninjured controls 6 and 12 months post-ACLR	1382:1425	ACLR-T1ρ Low exhibited greater KAM than uninjured controls 6 and 12 months post-ACLR.
35700436	4	93	theme	Gait	765:768	arg1	biomechanics					770:781	Gait biomechanics	765:781	METHODS Gait biomechanics	757:781	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	2	94	theme	insufficient	457:468	arg1	loading					481:487	excessive or insufficient knee joint loading	444:487	excessive or insufficient knee joint loading	444:487	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	2	95	theme	T1ρ	510:512	arg1	times					525:529	greater T1ρ relaxation times	502:529	greater T1ρ relaxation times 12 months post-ACLR	502:549	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	4	96	theme	%	826:826	arg1	females					828:834	50% females	824:834	50% females	824:834	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	96	theme	%	826:826	arg1	controls					814:821	26 uninjured controls	801:821	26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 )	801:889	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	2	97	theme	excessive	444:452	arg1	loading					481:487	excessive or insufficient knee joint loading	444:487	excessive or insufficient knee joint loading	444:487	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	0	98	theme	Insufficient	60:71	arg1	Kinetics					78:85	Insufficient Gait Kinetics	60:85	Insufficient Gait Kinetics After ACL Reconstruction	60:110	Worse Tibiofemoral Cartilage Composition Is Associated with Insufficient Gait Kinetics After ACL Reconstruction.
35700436	11	99	theme	insufficient	1898:1909	arg1	post-ACLR					1924:1932	insufficient KAM 6 months post-ACLR	1898:1932	insufficient KAM 6 months post-ACLR	1898:1932	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	3	100	theme	high	680:683	arg1	profiles					724:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35700436	5	101	theme	High	1028:1031	arg1	groups					1069:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups	1019:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups	1019:1074	ACLR-T1ρ High ( n = 9) and ACLR-T1ρ Low ( n = 17) groups were created based on 12-month post-ACLR T1ρ relaxation times using a k-means cluster analysis.
35700436	7	102	theme	post-ACLR	1331:1339	arg1	controls					1313:1320	ACLR-T1ρ Low and uninjured controls	1286:1320	ACLR-T1ρ Low and uninjured controls 6 months post-ACLR	1286:1339	RESULTS ACLR-T1ρ High exhibited lesser KAM than ACLR-T1ρ Low and uninjured controls 6 months post-ACLR.
35700436	8	103	theme	post-ACLR	1417:1425	arg1	controls					1392:1399	uninjured controls	1382:1399	uninjured controls 6 and 12 months post-ACLR	1382:1425	ACLR-T1ρ Low exhibited greater KAM than uninjured controls 6 and 12 months post-ACLR.
35700436	4	104	theme	uninjured	804:812	arg1	females					828:834	50% females	824:834	50% females	824:834	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	4	104	theme	uninjured	804:812	arg1	controls					814:821	26 uninjured controls	801:821	26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 )	801:889	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	2	105	theme	relaxation	371:380	arg1	times					382:386	T1ρ relaxation times	367:386	T1ρ relaxation times	367:386	Although changes in T1ρ relaxation times are associated with gait biomechanics, it is unclear if excessive or insufficient knee joint loading is linked to greater T1ρ relaxation times 12 months post-ACLR.
35700436	1	106	theme	osteoarthritis	264:277	arg1	development					279:289	posttraumatic osteoarthritis development	250:289	posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR)	250:344	PURPOSE Greater articular cartilage T1ρ magnetic resonance imaging relaxation times indicate less proteoglycan density and are linked to posttraumatic osteoarthritis development after anterior cruciate ligament reconstruction (ACLR).
35700436	11	107	from	increases	1802:1810	arg1	KAM					1815:1817	KAM	1815:1817	KAM	1815:1817	CONCLUSIONS Associations between worse T1ρ profiles and increases in KAM may be driven by the normalization of KAM in individuals who initially exhibit insufficient KAM 6 months post-ACLR.
35700436	4	108	theme	±	973:973	arg1	kg·m					979:982	24.2 ± 3.5 kg·m -2	968:985	24.2 ± 3.5 kg·m -2	968:985	METHODS Gait biomechanics were collected in 26 uninjured controls (50% females; age, 22 ± 4 yr; body mass index, 23.9 ± 2.8 kg·m -2 ) and 26 individuals after ACLR (50% females; age, 22 ± 4 yr; body mass index, 24.2 ± 3.5 kg·m -2 ) at 6 and 12 months post-ACLR.
35700436	3	109	theme	T1ρ	709:711	arg1	profiles					724:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	high versus low tibiofemoral T1ρ relaxation profiles	680:731	The purpose of this study was to compare external knee adduction (KAM) and flexion (KFM) moments in individuals after ACLR with high versus low tibiofemoral T1ρ relaxation profiles and uninjured controls.
35304323	16	0	theme	bone	2258:2261	arg1	function					2242:2249	a load-bearing function	2227:2249	a load-bearing function of the bone	2227:2261	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	12	1	theme	Further	1663:1669	arg1	work					1671:1674	Further work	1663:1674	Further work	1663:1674	Further work will be necessary to improve the loading of β-TCP and the bone infection organ model itself.
35304323	1	2	theme	bone	386:389	arg1	tissue					391:396	infected human bone tissue	371:396	infected human bone tissue	371:396	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	3	theme	microporous	216:226	arg1	ceramic					251:257	a microporous β-tricalcium phosphate ceramic	214:257	a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin	214:366	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	4	4	theme	flow	780:783	arg1	chamber					785:791	a flow chamber	778:791	a flow chamber	778:791	The loading of the composites was carried out by means of a flow chamber.
35304323	10	5	theme	ex-vivo	1448:1454	arg1	model					1477:1481	an ex-vivo human bone infection model	1445:1481	an ex-vivo human bone infection model	1445:1481	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	13	6	theme	infections	1825:1834	arg1	debridement					1839:1849	debridement	1839:1849	debridement	1839:1849	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	13	6	theme	infections	1825:1834	arg1	treatment					1807:1815	The common treatment	1796:1815	The common treatment of bone infections	1796:1834	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	5	7	from	material	884:891	arg1	28					917:918	28	917:918	28	917:918	The infection was observed for 28 days, quantifying bacteria in the medium and the osseus material on day 1, 7, 14, 21 and 28.
35304323	5	7	from	material	884:891	arg1	medium					862:867	the medium	858:867	the medium	858:867	The infection was observed for 28 days, quantifying bacteria in the medium and the osseus material on day 1, 7, 14, 21 and 28.
35304323	5	7	from	material	884:891	arg1	day					896:898	day 1, 7, 14, 21 and 28	896:918	day	896:898	The infection was observed for 28 days, quantifying bacteria in the medium and the osseus material on day 1, 7, 14, 21 and 28.
35304323	1	8	theme	phosphate	241:249	arg1	ceramic					251:257	a microporous β-tricalcium phosphate ceramic	214:257	a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin	214:366	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	2	9	theme	material	543:550	arg1	source					528:533	a source	526:533	a source of bone material	526:550	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	2	9	theme	material	543:550	arg1	plateaus					454:461	human tibia plateaus	442:461	human tibia plateaus	442:461	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	9	10	from	differences	1399:1409	arg1	vitality					1419:1426	bone vitality	1414:1426	bone vitality among groups	1414:1439	No significant differences between groups could be observed in the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups.
35304323	3	11	theme	loaded	629:634	arg1	composites					642:651	differently loaded β-TCP composites	617:651	differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded)	617:717	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	15	12	theme	treatment	2029:2037	arg1	option					2039:2044	another treatment option	2021:2044	another treatment option	2021:2044	Our work is intended to show another treatment option.
35304323	18	13	dep	a	2475:2475	arg1	substitute					2477:2486	substitute	2477:2486	substitute for previously used animal experiments	2477:2525	More importantly, it can also serve as a substitute for previously used animal experiments.
35304323	8	14	theme	bacterial	1099:1107	arg1	counts					1109:1114	bacterial counts	1099:1114	bacterial counts	1099:1114	In addition, a strong reduction in bacterial counts was recorded in the groups treated with ADA-gelatin + clindamycin and alginate + clindamycin, while the bacterial count in the control groups remained constant.
35304323	13	15	theme	common	1800:1805	arg1	debridement					1839:1849	debridement	1839:1849	debridement	1839:1849	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	13	15	theme	common	1800:1805	arg1	treatment					1807:1815	The common treatment	1796:1815	The common treatment of bone infections	1796:1834	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	1	16	theme	samples	157:163	arg1	infection					133:141	staphylococcal infection	118:141	staphylococcal infection of human bone samples	118:163	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	2	17	theme	total	481:485	arg1	surgery					504:510	total knee replacement surgery	481:510	total knee replacement surgery	481:510	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	10	18	theme	bone	1462:1465	arg1	model					1477:1481	an ex-vivo human bone infection model	1445:1481	an ex-vivo human bone infection model	1445:1481	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	9	19	theme	lacunae	1354:1360	arg1	number					1344:1349	the number	1340:1349	the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups	1340:1439	No significant differences between groups could be observed in the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups.
35304323	8	20	from	reduction	1086:1094	arg1	counts					1109:1114	bacterial counts	1099:1114	bacterial counts	1099:1114	In addition, a strong reduction in bacterial counts was recorded in the groups treated with ADA-gelatin + clindamycin and alginate + clindamycin, while the bacterial count in the control groups remained constant.
35304323	2	21	theme	replacement	492:502	arg1	surgery					504:510	total knee replacement surgery	481:510	total knee replacement surgery	481:510	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	1	22	theme	infected	371:378	arg1	tissue					391:396	infected human bone tissue	371:396	infected human bone tissue	371:396	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	23	theme	organ	102:106	arg1	model					108:112	an organ model	99:112	an organ model for staphylococcal infection of human bone samples	99:163	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	17	24	theme	musculoskeletal	2412:2426	arg1	system					2428:2433	the musculoskeletal system	2408:2433	the musculoskeletal system	2408:2433	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	13	25	theme	SIGNIFICANCE	1782:1793	arg1	STATEMENT					1769:1777	STATEMENT	1769:1777	STATEMENT OF SIGNIFICANCE	1769:1793	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	16	26	dep	developed	2068:2076	arg1	made					2079:2082	made	2079:2082	made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable	2079:2282	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	3	27	dep	alginate	654:661	arg1	unloaded					709:716	unloaded	709:716	unloaded	709:716	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	3	27	dep	alginate	654:661	arg1	clindamycin					696:706	clindamycin	696:706	clindamycin	696:706	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	3	27	dep	alginate	654:661	arg1	ADA-gelatin					680:690	ADA-gelatin	680:690	ADA-gelatin	680:690	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	16	28	theme	substance	2144:2152	arg1	carrier					2154:2160	the active substance carrier	2133:2160	the active substance carrier	2133:2160	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	9	29	theme	bone	1414:1417	arg1	vitality					1419:1426	bone vitality	1414:1426	bone vitality among groups	1414:1439	No significant differences between groups could be observed in the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups.
35304323	8	30	theme	control	1243:1249	arg1	groups					1251:1256	the control groups	1239:1256	the control groups	1239:1256	In addition, a strong reduction in bacterial counts was recorded in the groups treated with ADA-gelatin + clindamycin and alginate + clindamycin, while the bacterial count in the control groups remained constant.
35304323	2	31	theme	human	442:446	arg1	source					528:533	a source	526:533	a source of bone material	526:550	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	2	31	theme	human	442:446	arg1	plateaus					454:461	human tibia plateaus	442:461	human tibia plateaus	442:461	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	7	32	theme	organ	1051:1055	arg1	model					1057:1061	the organ model	1047:1061	the organ model	1047:1061	Bone infection could be consistently performed within the organ model.
35304323	12	33	theme	infection	1739:1747	arg1	model					1755:1759	the bone infection organ model	1730:1759	the bone infection organ model itself	1730:1766	Further work will be necessary to improve the loading of β-TCP and the bone infection organ model itself.
35304323	10	34	theme	days	1504:1507	arg1	period					1491:1496	a period	1489:1496	a period of 28 days	1489:1507	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	1	35	theme	ceramic	251:257	arg1	efficacy					202:209	efficacy	202:209	efficacy	202:209	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	35	theme	ceramic	251:257	arg1	influence					188:196	the influence	184:196	the influence	184:196	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	36	theme	human	380:384	arg1	tissue					391:396	infected human bone tissue	371:396	infected human bone tissue	371:396	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	17	37	theme	infections	2377:2386	arg1	analysis					2346:2353	analysis	2346:2353	analysis	2346:2353	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	17	37	theme	infections	2377:2386	arg1	treatment					2359:2367	treatment	2359:2367	treatment	2359:2367	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	16	38	theme	phosphate	2097:2105	arg1	ceramic					2107:2113	a calcium phosphate ceramic	2087:2113	a calcium phosphate ceramic	2087:2113	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	16	38	theme	phosphate	2097:2105	arg1	biodegradable					2270:2282	biodegradable	2270:2282	biodegradable	2270:2282	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	0	39	theme	bone	8:11	arg1	model					29:33	A human bone infection organ model	0:33	A human bone infection organ model for biomaterial research	0:58	A human bone infection organ model for biomaterial research.
35304323	12	40	theme	β-TCP	1720:1724	arg1	loading					1709:1715	the loading	1705:1715	the loading of β-TCP	1705:1724	Further work will be necessary to improve the loading of β-TCP and the bone infection organ model itself.
35304323	12	40	theme	β-TCP	1720:1724	arg1	model					1755:1759	the bone infection organ model	1730:1759	the bone infection organ model itself	1730:1766	Further work will be necessary to improve the loading of β-TCP and the bone infection organ model itself.
35304323	18	41	theme	used	2503:2506	arg1	experiments					2515:2525	previously used animal experiments	2492:2525	previously used animal experiments	2492:2525	More importantly, it can also serve as a substitute for previously used animal experiments.
35304323	0	42	theme	organ	23:27	arg1	model					29:33	A human bone infection organ model	0:33	A human bone infection organ model for biomaterial research	0:58	A human bone infection organ model for biomaterial research.
35304323	1	43	theme	RMS	267:269	arg1	β-TCP					260:264	β-TCP	260:264	β-TCP	260:264	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	43	theme	RMS	267:269	arg1	Foundation					271:280	RMS Foundation	267:280	RMS Foundation	267:280	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	14	44	theme	antibiotic-containing	1910:1930	arg1	carriers					1932:1939	antibiotic-containing carriers	1910:1939	antibiotic-containing carriers	1910:1939	In some cases, antibiotic-containing carriers are already used, but these must be removed again.
35304323	4	45	theme	composites	739:748	arg1	loading					724:730	The loading	720:730	The loading of the composites	720:748	The loading of the composites was carried out by means of a flow chamber.
35304323	13	46	theme	antibiotics	1882:1892	arg1	debridement					1839:1849	debridement	1839:1849	debridement	1839:1849	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	13	46	theme	antibiotics	1882:1892	arg1	administration					1864:1877	systemic administration	1855:1877	systemic administration of antibiotics	1855:1892	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	13	46	theme	antibiotics	1882:1892	arg1	treatment					1807:1815	The common treatment	1796:1815	The common treatment of bone infections	1796:1834	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	7	47	theme	Bone	993:996	arg1	infection					998:1006	Bone infection	993:1006	Bone infection	993:1006	Bone infection could be consistently performed within the organ model.
35304323	3	48	dep	composites	642:651	arg1	clindamycin					667:677	clindamycin	667:677	clindamycin	667:677	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	3	48	dep	composites	642:651	arg1	alginate					654:661	alginate	654:661	alginate	654:661	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	6	49	theme	vitality	972:979	arg1	evaluation					981:990	bone vitality evaluation	967:990	bone vitality evaluation	967:990	All samples were histologically processed for bone vitality evaluation.
35304323	5	50	from	bacteria	846:853	arg1	28					917:918	28	917:918	28	917:918	The infection was observed for 28 days, quantifying bacteria in the medium and the osseus material on day 1, 7, 14, 21 and 28.
35304323	5	50	from	bacteria	846:853	arg1	medium					862:867	the medium	858:867	the medium	858:867	The infection was observed for 28 days, quantifying bacteria in the medium and the osseus material on day 1, 7, 14, 21 and 28.
35304323	5	50	from	bacteria	846:853	arg1	day					896:898	day 1, 7, 14, 21 and 28	896:918	day	896:898	The infection was observed for 28 days, quantifying bacteria in the medium and the osseus material on day 1, 7, 14, 21 and 28.
35304323	5	51	theme	osseus	877:882	arg1	material					884:891	the osseus material	873:891	the osseus material on day 1, 7, 14, 21 and 28	873:918	The infection was observed for 28 days, quantifying bacteria in the medium and the osseus material on day 1, 7, 14, 21 and 28.
35304323	3	52	theme	S.	580:581	arg1	ATCC29213					590:598	S. aureus ATCC29213	580:598	S. aureus ATCC29213	580:598	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	16	53	theme	load-bearing	2229:2240	arg1	function					2242:2249	a load-bearing function	2227:2249	a load-bearing function of the bone	2227:2261	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	1	54	theme	human	146:150	arg1	samples					157:163	human bone samples	146:163	human bone samples	146:163	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	55	dep	-gelatin	342:349	arg1	alginate-di-aldehyde					316:335	alginate, alginate-di-aldehyde (ADA)-gelatin	306:349	alginate-di-aldehyde	316:335	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	10	56	theme	-releasing	1589:1598	arg1	composites					1606:1615	-releasing β-TCP composites	1589:1615	-releasing β-TCP composites	1589:1615	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	9	57	theme	significant	1280:1290	arg1	differences					1292:1302	No significant differences	1277:1302	No significant differences between groups	1277:1317	No significant differences between groups could be observed in the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups.
35304323	10	58	theme	human	1456:1460	arg1	model					1477:1481	an ex-vivo human bone infection model	1445:1481	an ex-vivo human bone infection model	1445:1481	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	13	59	theme	bone	1820:1823	arg1	infections					1825:1834	bone infections	1820:1834	bone infections	1820:1834	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	1	60	theme	β-tricalcium	228:239	arg1	ceramic					251:257	a microporous β-tricalcium phosphate ceramic	214:257	a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin	214:366	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	9	61	located	observed	1328:1335	arg1	number					1344:1349	the number	1340:1349	the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups	1340:1439	No significant differences between groups could be observed in the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups.
35304323	9	61	located	observed	1328:1335	arg2	differences					1292:1302	No significant differences	1277:1302	No significant differences between groups	1277:1317	No significant differences between groups could be observed in the number of lacunae filled with osteocytes suggesting no differences in bone vitality among groups.
35304323	17	62	dep	model	2302:2306	arg1	we					2308:2309	we	2308:2309	we	2308:2309	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	2	63	theme	bone	538:541	arg1	material					543:550	bone material	538:550	bone material	538:550	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	8	64	theme	strong	1079:1084	arg1	reduction					1086:1094	a strong reduction	1077:1094	a strong reduction in bacterial counts	1077:1114	In addition, a strong reduction in bacterial counts was recorded in the groups treated with ADA-gelatin + clindamycin and alginate + clindamycin, while the bacterial count in the control groups remained constant.
35304323	3	65	theme	β-TCP	636:640	arg1	composites					642:651	differently loaded β-TCP composites	617:651	differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded)	617:717	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	1	66	theme	bone	152:155	arg1	samples					157:163	human bone samples	146:163	human bone samples	146:163	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	10	67	theme	infection	1467:1475	arg1	model					1477:1481	an ex-vivo human bone infection model	1445:1481	an ex-vivo human bone infection model	1445:1481	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	1	68	theme	days	418:421	arg1	period					405:410	a period	403:410	a period of 28 days	403:421	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	17	69	theme	other	2388:2392	arg1	infections					2377:2386	bone infections	2372:2386	bone infections other than those of the musculoskeletal system	2372:2433	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	2	70	used	used	518:521	arg2	plateaus					454:461	human tibia plateaus	442:461	human tibia plateaus	442:461	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	2	70	used	used	518:521	arg2	source					528:533	a source	526:533	a source of bone material	526:550	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	1	71	from	efficacy	202:209	arg1	tissue					391:396	infected human bone tissue	371:396	infected human bone tissue	371:396	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	17	72	dep	analysis	2346:2353	arg1	the					2342:2344	the	2342:2344	the	2342:2344	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	16	73	theme	active	2197:2202	arg1	substance					2204:2212	the active substance	2193:2212	the active substance	2193:2212	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	1	74	from	influence	188:196	arg1	tissue					391:396	infected human bone tissue	371:396	infected human bone tissue	371:396	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	2	75	theme	knee	487:490	arg1	surgery					504:510	total knee replacement surgery	481:510	total knee replacement surgery	481:510	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	16	76	theme	active	2137:2142	arg1	carrier					2154:2160	the active substance carrier	2133:2160	the active substance carrier	2133:2160	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	14	77	used	used	1953:1956	arg2	carriers					1932:1939	antibiotic-containing carriers	1910:1939	antibiotic-containing carriers	1910:1939	In some cases, antibiotic-containing carriers are already used, but these must be removed again.
35304323	1	78	theme	staphylococcal	118:131	arg1	infection					133:141	staphylococcal infection	118:141	staphylococcal infection of human bone samples	118:163	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	79	dep	ceramic	251:257	arg1	β-TCP					260:264	β-TCP	260:264	β-TCP	260:264	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	79	dep	ceramic	251:257	arg1	Foundation					271:280	RMS Foundation	267:280	RMS Foundation	267:280	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	17	80	used	used	2333:2336	arg2	model					2302:2306	the model	2298:2306	the model we developed	2298:2319	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	18	81	theme	animal	2508:2513	arg1	experiments					2515:2525	previously used animal experiments	2492:2525	previously used animal experiments	2492:2525	More importantly, it can also serve as a substitute for previously used animal experiments.
35304323	11	82	theme	clinical	1649:1656	arg1	use					1658:1660	its clinical use	1645:1660	its clinical use	1645:1660	This could be relevant for its clinical use.
35304323	16	83	dep	scaffold	2051:2058	arg1	we					2060:2061	we	2060:2061	we	2060:2061	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	1	84	theme	alginate	306:313	arg1	-gelatin					342:349	alginate, alginate-di-aldehyde (ADA)-gelatin	306:349	-gelatin	342:349	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	84	theme	alginate	306:313	arg1	hydrogels					295:303	hydrogels	295:303	hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin)	295:350	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	2	85	theme	tibia	448:452	arg1	source					528:533	a source	526:533	a source of bone material	526:550	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	2	85	theme	tibia	448:452	arg1	plateaus					454:461	human tibia plateaus	442:461	human tibia plateaus	442:461	For this purpose, human tibia plateaus, collected during total knee replacement surgery, were used as a source of bone material.
35304323	12	86	theme	organ	1749:1753	arg1	model					1755:1759	the bone infection organ model	1730:1759	the bone infection organ model itself	1730:1766	Further work will be necessary to improve the loading of β-TCP and the bone infection organ model itself.
35304323	16	87	dep	releasing	2183:2191	arg1	addition					2171:2178	addition	2171:2178	addition	2171:2178	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	8	88	theme	bacterial	1220:1228	arg1	count					1230:1234	the bacterial count	1216:1234	the bacterial count in the control groups	1216:1256	In addition, a strong reduction in bacterial counts was recorded in the groups treated with ADA-gelatin + clindamycin and alginate + clindamycin, while the bacterial count in the control groups remained constant.
35304323	16	89	theme	calcium	2089:2095	arg1	ceramic					2107:2113	a calcium phosphate ceramic	2087:2113	a calcium phosphate ceramic	2087:2113	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	16	89	theme	calcium	2089:2095	arg1	biodegradable					2270:2282	biodegradable	2270:2282	biodegradable	2270:2282	The scaffold we have developed, made of a calcium phosphate ceramic and a hydrogel as the active substance carrier, can, in addition to releasing the active substance, also assume a load-bearing function of the bone and is biodegradable.
35304323	10	90	theme	bacterial	1509:1517	arg1	growth					1519:1524	bacterial growth	1509:1524	bacterial growth	1509:1524	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	0	91	theme	human	2:6	arg1	model					29:33	A human bone infection organ model	0:33	A human bone infection organ model for biomaterial research	0:58	A human bone infection organ model for biomaterial research.
35304323	1	92	theme	ADA	338:340	arg1	-gelatin					342:349	alginate, alginate-di-aldehyde (ADA)-gelatin	306:349	-gelatin	342:349	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	1	92	theme	ADA	338:340	arg1	hydrogels					295:303	hydrogels	295:303	hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin)	295:350	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	17	93	theme	bone	2372:2375	arg1	infections					2377:2386	bone infections	2372:2386	bone infections other than those of the musculoskeletal system	2372:2433	In addition, the model we developed can also be used for the analysis and treatment of bone infections other than those of the musculoskeletal system.
35304323	0	94	theme	infection	13:21	arg1	model					29:33	A human bone infection organ model	0:33	A human bone infection organ model for biomaterial research	0:58	A human bone infection organ model for biomaterial research.
35304323	1	95	theme	work	77:80	arg1	aim					65:67	The aim	61:67	The aim of this work	61:80	The aim of this work was to establish an organ model for staphylococcal infection of human bone samples and to investigate the influence and efficacy of a microporous β-tricalcium phosphate ceramic (β-TCP, RMS Foundation) loaded with hydrogels (alginate, alginate-di-aldehyde (ADA)-gelatin) and clindamycin on infected human bone tissue over a period of 28 days.
35304323	10	96	dep	ADA-Gel + CLI	1561:1573	arg1	composites					1606:1615	-releasing β-TCP composites	1589:1615	-releasing β-TCP composites	1589:1615	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	12	97	theme	bone	1734:1737	arg1	model					1755:1759	the bone infection organ model	1730:1759	the bone infection organ model itself	1730:1766	Further work will be necessary to improve the loading of β-TCP and the bone infection organ model itself.
35304323	10	98	with	treatment	1546:1554	arg1	ADA-Gel + CLI					1561:1573	ADA-Gel + CLI	1561:1573	ADA-Gel + CLI	1561:1573	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	10	98	with	treatment	1546:1554	arg1	ALG + CLI					1579:1587	ALG + CLI	1579:1587	ALG + CLI	1579:1587	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35304323	0	99	theme	biomaterial	39:49	arg1	research					51:58	biomaterial research	39:58	biomaterial research	39:58	A human bone infection organ model for biomaterial research.
35304323	3	100	theme	aureus	583:588	arg1	ATCC29213					590:598	S. aureus ATCC29213	580:598	S. aureus ATCC29213	580:598	Samples were infected with S. aureus ATCC29213 and treated with differently loaded β-TCP composites (alginate +/- clindamycin, ADA-gelatin +/- clindamycin, unloaded).
35304323	8	101	from	count	1230:1234	arg1	groups					1251:1256	the control groups	1239:1256	the control groups	1239:1256	In addition, a strong reduction in bacterial counts was recorded in the groups treated with ADA-gelatin + clindamycin and alginate + clindamycin, while the bacterial count in the control groups remained constant.
35304323	6	102	theme	bone	967:970	arg1	evaluation					981:990	bone vitality evaluation	967:990	bone vitality evaluation	967:990	All samples were histologically processed for bone vitality evaluation.
35304323	13	103	theme	systemic	1855:1862	arg1	administration					1864:1877	systemic administration	1855:1877	systemic administration of antibiotics	1855:1892	STATEMENT OF SIGNIFICANCE: The common treatment of bone infections is debridement and systemic administration of antibiotics.
35304323	10	104	theme	β-TCP	1600:1604	arg1	composites					1606:1615	-releasing β-TCP composites	1589:1615	-releasing β-TCP composites	1589:1615	In an ex-vivo human bone infection model, over a period of 28 days bacterial growth could be reduced by treatment with ADA-Gel + CLI and ALG + CLI -releasing β-TCP composites.
35084790	0	0	theme	oxidative	88:96	arg1	stress					98:103	oxidative stress	88:103	oxidative stress	88:103	Costunolide protects against alcohol-induced liver injury by regulating gut microbiota, oxidative stress and attenuating inflammation in vivo and in vitro.
35084790	9	1	theme	ALI	1619:1621	arg1	treatment					1606:1614	treatment	1606:1614	treatment of ALI	1606:1621	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	6	2	used	used	874:877	arg2	levels					794:799	the levels	790:799	the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST	790:912	Plasma was separated from blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST.
35084790	4	3	used	used	593:596	arg2	silymarin					579:587	silymarin	579:587	silymarin	579:587	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
35084790	4	3	used	used	593:596	arg2	control					610:616	positive control	601:616	positive control	601:616	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
35084790	9	4	theme	active	1577:1582	arg1	pathway					1550:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	4	theme	active	1577:1582	arg1	agent					1596:1600	an active therapeutic agent	1574:1600	an active therapeutic agent for treatment of ALI	1574:1621	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	5	theme	higher	1200:1205	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	5	theme	higher	1200:1205	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	6	theme	relative	1207:1214	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	6	theme	relative	1207:1214	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	7	dep	stress	1329:1334	arg1	reduced					1337:1343	reduced	1337:1343	reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells	1337:1422	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	4	8	theme	potential	639:647	arg1	mechanisms					649:658	its potential mechanisms	635:658	its potential mechanisms	635:658	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
35084790	9	9	from	Proteobacteria	1292:1305	arg1	Bacteroidetes					1274:1286	Bacteroidetes	1274:1286	Bacteroidetes	1274:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	10	theme	TNF-α	1510:1514	arg1	levels					1469:1474	the levels	1465:1474	the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α	1465:1514	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	11	theme	liver	1146:1150	arg1	injury					1152:1157	alcoholic-induced liver injury	1128:1157	alcoholic-induced liver injury	1128:1157	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	6	12	theme	IL-6	811:814	arg1	levels					794:799	the levels	790:799	the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST	790:912	Plasma was separated from blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST.
35084790	8	13	theme	feces	1015:1019	arg1	samples					1021:1027	Fresh mice feces samples	1004:1027	Fresh mice feces samples	1004:1027	Fresh mice feces samples were collected for the detection of bacterial composition.
35084790	9	14	theme	signaling	1540:1548	arg1	agent					1596:1600	an active therapeutic agent	1574:1600	an active therapeutic agent for treatment of ALI	1574:1621	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	14	theme	signaling	1540:1548	arg1	pathway					1550:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	15	theme	L-02	1413:1416	arg1	cells					1418:1422	L-02 cells	1413:1422	L-02 cells	1413:1422	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	16	theme	LPS-TLR4-NF-κB	1521:1534	arg1	agent					1596:1600	an active therapeutic agent	1574:1600	an active therapeutic agent for treatment of ALI	1574:1621	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	16	theme	LPS-TLR4-NF-κB	1521:1534	arg1	pathway					1550:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	2	17	theme	Dolomiaea	250:258	arg1	genus					260:264	the Dolomiaea genus	246:264	the Dolomiaea genus in the family Compositae	246:289	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	4	18	theme	hepatoprotective	546:561	arg1	effects					563:569	the hepatoprotective effects	542:569	the hepatoprotective effects of cos	542:576	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
35084790	2	19	theme	anti-inebriation	302:317	arg1	effect					319:324	the anti-inebriation effect	298:324	the anti-inebriation effect	298:324	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	7	20	theme	western	981:987	arg1	analysis					994:1001	western blot analysis	981:1001	western blot analysis	981:1001	Liver tissues were obtained for histopathological examination and western blot analysis.
35084790	3	21	theme	cos	438:440	arg1	effect					428:433	the protective effect	413:433	the protective effect of cos against alcohol-induced liver injury (ALI)	413:483	However, the protective effect of cos against alcohol-induced liver injury (ALI) remains obscure.
35084790	9	22	from	GSH-PX	1403:1408	arg1	cells					1418:1422	L-02 cells	1413:1422	L-02 cells	1413:1422	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	2	23	theme	alcohol	365:371	arg1	absorption					351:360	the absorption	347:360	the absorption of alcohol in the gastrointestinal tract	347:401	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	0	24	theme	attenuating	109:119	arg1	inflammation					121:132	attenuating inflammation	109:132	attenuating inflammation	109:132	Costunolide protects against alcohol-induced liver injury by regulating gut microbiota, oxidative stress and attenuating inflammation in vivo and in vitro.
35084790	5	25	theme	cell	710:713	arg1	viability					715:723	the cell viability	706:723	the cell viability of L-02 cells	706:737	MTT was used to examine the effect of cos on the cell viability of L-02 cells.
35084790	9	26	theme	oxidative	1319:1327	arg1	stress					1329:1334	oxidative stress	1319:1334	oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells)	1319:1423	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	6	27	theme	LPS	831:833	arg1	levels					794:799	the levels	790:799	the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST	790:912	Plasma was separated from blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST.
35084790	5	28	theme	cells	733:737	arg1	viability					715:723	the cell viability	706:723	the cell viability of L-02 cells	706:737	MTT was used to examine the effect of cos on the cell viability of L-02 cells.
35084790	9	29	theme	Actinobacteria	1244:1257	arg1	Proteobacteria					1292:1305	Proteobacteria	1292:1305	Proteobacteria	1292:1305	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	29	theme	Actinobacteria	1244:1257	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	29	theme	Actinobacteria	1244:1257	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	30	theme	protective	1102:1111	arg1	effect					1113:1118	protective effect	1102:1118	protective effect	1102:1118	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	2	31	dep	exert	292:296	arg1	belongs					235:241	belongs	235:241	belongs to the Dolomiaea genus in the family Compositae	235:289	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	6	32	theme	TNF-α	804:808	arg1	levels					794:799	the levels	790:799	the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST	790:912	Plasma was separated from blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST.
35084790	9	33	theme	ROS	1371:1373	arg1	activities					1349:1358	the activities	1345:1358	the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells	1345:1422	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	8	34	theme	bacterial	1065:1073	arg1	composition					1075:1085	bacterial composition	1065:1085	bacterial composition	1065:1085	Fresh mice feces samples were collected for the detection of bacterial composition.
35084790	2	35	theme	family	273:278	arg1	Compositae					280:289	the family Compositae	269:289	the family Compositae	269:289	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	5	36	from	effect	689:694	arg1	viability					715:723	the cell viability	706:723	the cell viability of L-02 cells	706:737	MTT was used to examine the effect of cos on the cell viability of L-02 cells.
35084790	0	37	theme	alcohol-induced	29:43	arg1	injury					51:56	alcohol-induced liver injury	29:56	alcohol-induced liver injury	29:56	Costunolide protects against alcohol-induced liver injury by regulating gut microbiota, oxidative stress and attenuating inflammation in vivo and in vitro.
35084790	9	38	theme	MDA	1363:1365	arg1	activities					1349:1358	the activities	1345:1358	the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells	1345:1422	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	39	theme	ALT	1479:1481	arg1	levels					1469:1474	the levels	1465:1474	the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α	1465:1514	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	40	theme	AST	1484:1486	arg1	levels					1469:1474	the levels	1465:1474	the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α	1465:1514	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	41	theme	promoted	1381:1388	arg1	SOD					1390:1392	promoted SOD	1381:1392	promoted SOD	1381:1392	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	7	42	theme	histopathological	947:963	arg1	examination					965:975	histopathological examination	947:975	histopathological examination	947:975	Liver tissues were obtained for histopathological examination and western blot analysis.
35084790	9	43	theme	alcoholic-induced	1128:1144	arg1	injury					1152:1157	alcoholic-induced liver injury	1128:1157	alcoholic-induced liver injury	1128:1157	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	44	theme	attenuating	1429:1439	arg1	inflammation					1441:1452	attenuating inflammation	1429:1452	attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α)	1429:1515	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	2	45	theme	gastrointestinal	380:395	arg1	tract					397:401	the gastrointestinal tract	376:401	the gastrointestinal tract	376:401	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	5	46	used	used	669:672	arg2	MTT					661:663	MTT	661:663	MTT	661:663	MTT was used to examine the effect of cos on the cell viability of L-02 cells.
35084790	9	47	theme	Firmicutes	1229:1238	arg1	Proteobacteria					1292:1305	Proteobacteria	1292:1305	Proteobacteria	1292:1305	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	47	theme	Firmicutes	1229:1238	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	47	theme	Firmicutes	1229:1238	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	4	48	theme	positive	601:608	arg1	control					610:616	positive control	601:616	positive control	601:616	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
35084790	4	48	theme	positive	601:608	arg1	silymarin					579:587	silymarin	579:587	silymarin	579:587	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
35084790	1	49	dep	derived	174:180	arg1	Franch					219:224	Franch	219:224	Franch	219:224	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	1	49	dep	derived	174:180	arg1	Costunolide					156:166	Costunolide	156:166	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)	156:226	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	3	50	theme	protective	417:426	arg1	effect					428:433	the protective effect	413:433	the protective effect of cos against alcohol-induced liver injury (ALI)	413:483	However, the protective effect of cos against alcohol-induced liver injury (ALI) remains obscure.
35084790	0	51	theme	liver	45:49	arg1	injury					51:56	alcohol-induced liver injury	29:56	alcohol-induced liver injury	29:56	Costunolide protects against alcohol-induced liver injury by regulating gut microbiota, oxidative stress and attenuating inflammation in vivo and in vitro.
35084790	9	52	from	abundance	1216:1224	arg1	Bacteroidetes					1274:1286	Bacteroidetes	1274:1286	Bacteroidetes	1274:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	2	53	from	absorption	351:360	arg1	tract					397:401	the gastrointestinal tract	376:401	the gastrointestinal tract	376:401	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	1	54	theme	Dolomiaea	200:208	arg1	souliei					210:216	Dolomiaea souliei	200:216	Dolomiaea souliei	200:216	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	9	55	dep	capacities	1188:1197	arg1	Proteobacteria					1292:1305	Proteobacteria	1292:1305	Proteobacteria	1292:1305	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	55	dep	capacities	1188:1197	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	55	dep	capacities	1188:1197	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	7	56	theme	Liver	915:919	arg1	tissues					921:927	Liver tissues	915:927	Liver tissues	915:927	Liver tissues were obtained for histopathological examination and western blot analysis.
35084790	1	57	attach	derived	174:180	arg2	Franch					219:224	Franch	219:224	Franch	219:224	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	1	57	attach	derived	174:180	arg1	roots					191:195	the roots	187:195	the roots of Dolomiaea souliei	187:216	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	1	57	attach	derived	174:180	arg2	Costunolide					156:166	Costunolide	156:166	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)	156:226	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	1	57	attach	derived	174:180	arg2	cos					169:171	cos	169:171	cos	169:171	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	9	58	theme	LPS	1489:1491	arg1	levels					1469:1474	the levels	1465:1474	the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α	1465:1514	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	59	from	GSH	1395:1397	arg1	cells					1418:1422	L-02 cells	1413:1422	L-02 cells	1413:1422	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	3	60	theme	liver	466:470	arg1	ALI					480:482	ALI	480:482	ALI	480:482	However, the protective effect of cos against alcohol-induced liver injury (ALI) remains obscure.
35084790	3	60	theme	liver	466:470	arg1	injury					472:477	alcohol-induced liver injury	450:477	alcohol-induced liver injury (ALI)	450:483	However, the protective effect of cos against alcohol-induced liver injury (ALI) remains obscure.
35084790	5	61	theme	L-02	728:731	arg1	cells					733:737	L-02 cells	728:737	L-02 cells	728:737	MTT was used to examine the effect of cos on the cell viability of L-02 cells.
35084790	4	62	theme	present	506:512	arg1	study					514:518	The present study	502:518	The present study	502:518	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
35084790	9	63	theme	therapeutic	1584:1594	arg1	pathway					1550:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	63	theme	therapeutic	1584:1594	arg1	agent					1596:1600	an active therapeutic agent	1574:1600	an active therapeutic agent for treatment of ALI	1574:1621	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	7	64	theme	blot	989:992	arg1	analysis					994:1001	western blot analysis	981:1001	western blot analysis	981:1001	Liver tissues were obtained for histopathological examination and western blot analysis.
35084790	8	65	theme	composition	1075:1085	arg1	detection					1052:1060	the detection	1048:1060	the detection of bacterial composition	1048:1085	Fresh mice feces samples were collected for the detection of bacterial composition.
35084790	8	66	theme	mice	1010:1013	arg1	samples					1021:1027	Fresh mice feces samples	1004:1027	Fresh mice feces samples	1004:1027	Fresh mice feces samples were collected for the detection of bacterial composition.
35084790	9	67	theme	gut	1173:1175	arg1	Proteobacteria					1292:1305	Proteobacteria	1292:1305	Proteobacteria	1292:1305	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	67	theme	gut	1173:1175	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	67	theme	gut	1173:1175	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	68	theme	IL-6	1494:1497	arg1	levels					1469:1474	the levels	1465:1474	the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α	1465:1514	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	5	69	theme	cos	699:701	arg1	effect					689:694	the effect	685:694	the effect of cos on the cell viability of L-02 cells	685:737	MTT was used to examine the effect of cos on the cell viability of L-02 cells.
35084790	1	70	theme	souliei	210:216	arg1	roots					191:195	the roots	187:195	the roots of Dolomiaea souliei	187:216	Costunolide (cos) derived from the roots of Dolomiaea souliei (Franch.)
35084790	8	71	theme	Fresh	1004:1008	arg1	samples					1021:1027	Fresh mice feces samples	1004:1027	Fresh mice feces samples	1004:1027	Fresh mice feces samples were collected for the detection of bacterial composition.
35084790	9	72	theme	microbiota	1177:1186	arg1	Proteobacteria					1292:1305	Proteobacteria	1292:1305	Proteobacteria	1292:1305	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	72	theme	microbiota	1177:1186	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	72	theme	microbiota	1177:1186	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	3	73	theme	alcohol-induced	450:464	arg1	ALI					480:482	ALI	480:482	ALI	480:482	However, the protective effect of cos against alcohol-induced liver injury (ALI) remains obscure.
35084790	3	73	theme	alcohol-induced	450:464	arg1	injury					472:477	alcohol-induced liver injury	450:477	alcohol-induced liver injury (ALI)	450:483	However, the protective effect of cos against alcohol-induced liver injury (ALI) remains obscure.
35084790	2	74	from	genus	260:264	arg1	Compositae					280:289	the family Compositae	269:289	the family Compositae	269:289	, which belongs to the Dolomiaea genus in the family Compositae, exert the anti-inebriation effect mainly by inhibiting the absorption of alcohol in the gastrointestinal tract.
35084790	6	75	theme	ALT	902:904	arg1	level					893:897	the level	889:897	the level of ALT and AST	889:912	Plasma was separated from blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST.
35084790	6	76	theme	AST	910:912	arg1	level					893:897	the level	889:897	the level of ALT and AST	889:912	Plasma was separated from blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST.
35084790	9	77	theme	IL-12	1500:1504	arg1	levels					1469:1474	the levels	1465:1474	the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α	1465:1514	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	78	from	SOD	1390:1392	arg1	cells					1418:1422	L-02 cells	1413:1422	L-02 cells	1413:1422	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	6	79	used	used	777:780	arg2	blood					766:770	blood	766:770	blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST	766:912	Plasma was separated from blood that used to test the levels of TNF-α, IL-6 and IL-12, and LPS while serum separated from blood which used to detect the level of ALT and AST.
35084790	0	80	theme	gut	72:74	arg1	microbiota					76:85	gut microbiota	72:85	gut microbiota	72:85	Costunolide protects against alcohol-induced liver injury by regulating gut microbiota, oxidative stress and attenuating inflammation in vivo and in vitro.
35084790	9	81	theme	p65	1536:1538	arg1	agent					1596:1600	an active therapeutic agent	1574:1600	an active therapeutic agent for treatment of ALI	1574:1621	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	81	theme	p65	1536:1538	arg1	pathway					1550:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	LPS-TLR4-NF-κB p65 signaling pathway	1521:1556	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	82	from	Bacteroidetes	1274:1286	arg1	Proteobacteria					1292:1305	Proteobacteria	1292:1305	Proteobacteria	1292:1305	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	82	from	Bacteroidetes	1274:1286	arg1	abundance					1216:1224	higher relative abundance	1200:1224	higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes	1200:1286	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	9	82	from	Bacteroidetes	1274:1286	arg1	capacities					1188:1197	gut microbiota capacities	1173:1197	gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria)	1173:1306	Cos exhibited protective effect against alcoholic-induced liver injury by regulating gut microbiota capacities (higher relative abundance of Firmicutes and Actinobacteria while lower in Bacteroidetes and Proteobacteria), adjusting oxidative stress (reduced the activities of MDA and ROS while promoted SOD, GSH and GSH-PX in L-02 cells) and attenuating inflammation (decreased the levels of ALT, AST, LPS, IL-6, IL-12 and TNF-α) via LPS-TLR4-NF-κB p65 signaling pathway, which might be an active therapeutic agent for treatment of ALI.
35084790	4	83	theme	cos	574:576	arg1	effects					563:569	the hepatoprotective effects	542:569	the hepatoprotective effects of cos	542:576	The present study was aimed to evaluate the hepatoprotective effects of cos (silymarin was used as positive control) against ALI and its potential mechanisms.
36402938	4	0	theme	milk	745:748	arg1	kg					757:758	kg	757:758	kg	757:758	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	0	theme	milk	745:748	arg1	yield					750:754	milk yield	745:754	milk yield (kg)	745:759	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	5	1	theme	collateral	946:955	arg1	factors					957:963	collateral factors	946:963	collateral factors	946:963	Although the aforementioned factor combination significantly conditions milk production and composition, SCC may be particularly affected by collateral factors.
36402938	4	2	from	%	651:651	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	2	from	%	651:651	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	3	from	matter	779:784	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	3	from	matter	779:784	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	1	4	theme	production	247:256	arg1	variability					227:237	the variability	223:237	the variability of milk production and composition	223:272	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	9	5	theme	period-related	1489:1502	arg1	factors					1516:1522	drying period-related non-genetic factors	1482:1522	drying period-related non-genetic factors	1482:1522	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	6	6	theme	reference	1012:1020	arg1	factors					1000:1006	Milking routine and drying period factors	966:1006	factors	1000:1006	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	6	theme	reference	1012:1020	arg1	predictors					1022:1031	reference predictors	1012:1031	reference predictors	1012:1031	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	6	theme	reference	1012:1020	arg1	routine					974:980	Milking routine and drying period factors	966:1006	routine	974:980	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	7	theme	progression	1103:1113	arg1	evaluation					1057:1066	the evaluation	1053:1066	the evaluation of milk production and composition progression	1053:1113	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	3	8	theme	quality-related	523:537	arg1	nutrients					544:552	quality-related milk nutrients	523:552	quality-related milk nutrients	523:552	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	1	9	theme	study	164:168	arg1	aim					152:154	The aim	148:154	The aim of this study	148:168	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	4	10	from	fat	762:764	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	10	from	fat	762:764	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	11	from	%	637:637	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	11	from	%	637:637	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	9	12	theme	genetic	1527:1533	arg1	evaluations					1535:1545	genetic evaluations	1527:1545	genetic evaluations	1527:1545	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	7	13	theme	matter	1241:1246	arg1	contents					1248:1255	dry matter contents	1237:1255	dry matter contents	1237:1255	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	0	14	from	phenomics	64:72	arg1	evaluation					9:18	Bayesian evaluation	0:18	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.	0:146	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	6	15	theme	production	1076:1085	arg1	evaluation					1057:1066	the evaluation	1053:1066	the evaluation of milk production and composition progression	1053:1113	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	0	16	theme	Murciano-Granadina	122:139	arg1	goats					141:145	Murciano-Granadina goats	122:145	Murciano-Granadina goats	122:145	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	1	17	from	effect	190:195	arg1	variability					227:237	the variability	223:237	the variability of milk production and composition	223:272	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	8	18	theme	drying	1301:1306	arg1	years					1308:1312	drying years	1301:1312	drying years	1301:1312	Variability across drying years may depend on the drying season rather than the drying month course, except for milk yield, for which an increasing trend was reported from winter to summer.
36402938	5	19	theme	milk	877:880	arg1	production					882:891	milk production	877:891	milk production	877:891	Although the aforementioned factor combination significantly conditions milk production and composition, SCC may be particularly affected by collateral factors.
36402938	0	20	from	nutrients	99:107	arg1	goats					141:145	Murciano-Granadina goats	122:145	Murciano-Granadina goats	122:145	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	4	21	from	lactose	719:725	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	21	from	lactose	719:725	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	9	22	theme	models	1587:1592	arg1	accuracy					1560:1567	the accuracy	1556:1567	the accuracy of the regression models	1556:1592	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	4	23	dep	SCC	706:708	arg1	sc/mL					711:715	sc/mL	711:715	sc/mL	711:715	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	8	24	theme	drying	1332:1337	arg1	season					1339:1344	the drying season	1328:1344	the drying season rather than the drying month course	1328:1380	Variability across drying years may depend on the drying season rather than the drying month course, except for milk yield, for which an increasing trend was reported from winter to summer.
36402938	4	25	theme	dry	775:777	arg1	matter					779:784	dry matter	775:784	dry matter (%)	775:788	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	25	theme	dry	775:777	arg1	%					787:787	%	787:787	%	787:787	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	0	26	theme	quality-related	78:92	arg1	nutrients					99:107	quality-related milk nutrients	78:107	quality-related milk nutrients	78:107	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	9	27	theme	commercial	1619:1628	arg1	possibilities					1630:1642	the commercial possibilities	1615:1642	the commercial possibilities	1615:1642	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	3	28	theme	linear	427:432	arg1	regression					434:443	Bayesian linear regression	418:443	Bayesian linear regression	418:443	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	0	29	from	effect	27:32	arg1	phenomics					64:72	the phenomics	60:72	the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats	60:145	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	3	30	used	used	449:452	arg2	regression					434:443	Bayesian linear regression	418:443	Bayesian linear regression	418:443	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	0	31	theme	Bayesian	0:7	arg1	evaluation					9:18	Bayesian evaluation	0:18	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.	0:146	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	7	32	theme	lactose	1182:1188	arg1	content					1190:1196	lactose content	1182:1196	lactose content	1182:1196	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	4	33	theme	cell	694:697	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	33	theme	cell	694:697	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	2	34	theme	breed	411:415	arg1	nucleus					377:383	the breeding nucleus	364:383	the breeding nucleus of the Murciano-Granadina breed	364:415	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
36402938	7	35	theme	somatic	1262:1268	arg1	count					1275:1279	somatic cell count	1262:1279	somatic cell count	1262:1279	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	9	36	theme	breeds	1671:1676	arg1	profitability					1648:1660	profitability	1648:1660	profitability of local breeds	1648:1676	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	9	36	theme	breeds	1671:1676	arg1	possibilities					1630:1642	the commercial possibilities	1615:1642	the commercial possibilities	1615:1642	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	0	37	theme	effect	27:32	arg1	evaluation					9:18	Bayesian evaluation	0:18	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.	0:146	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	1	38	theme	linear	289:294	arg1	regression					296:305	Bayesian linear regression	280:305	Bayesian linear regression	280:305	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	0	39	theme	non-genetic	37:47	arg1	factors					49:55	non-genetic factors	37:55	non-genetic factors	37:55	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	2	40	from	nucleus	377:383	arg1	records					330:336	2594 milk records	320:336	2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed	320:415	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
36402938	6	41	theme	Milking	966:972	arg1	factors					1000:1006	Milking routine and drying period factors	966:1006	factors	1000:1006	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	41	theme	Milking	966:972	arg1	predictors					1022:1031	reference predictors	1012:1031	reference predictors	1012:1031	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	41	theme	Milking	966:972	arg1	routine					974:980	Milking routine and drying period factors	966:1006	routine	974:980	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	2	42	theme	2594	320:323	arg1	records					330:336	2594 milk records	320:336	2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed	320:415	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
36402938	1	43	theme	non-genetic	200:210	arg1	factors					212:218	non-genetic factors	200:218	non-genetic factors	200:218	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	7	44	theme	period	1123:1128	arg1	extensions					1130:1139	Drying period extensions	1116:1139	Drying period extensions	1116:1139	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	5	45	theme	aforementioned	818:831	arg1	combination					840:850	the aforementioned factor combination	814:850	the aforementioned factor combination	814:850	Although the aforementioned factor combination significantly conditions milk production and composition, SCC may be particularly affected by collateral factors.
36402938	0	46	from	yield	113:117	arg1	goats					141:145	Murciano-Granadina goats	122:145	Murciano-Granadina goats	122:145	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	3	47	theme	factors	494:500	arg1	effects					471:477	the effects	467:477	the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield	467:562	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	4	48	theme	regression	578:587	arg1	model					589:593	Multivariate regression model	565:593	Multivariate regression model	565:593	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	3	49	theme	milk	539:542	arg1	nutrients					544:552	quality-related milk nutrients	523:552	quality-related milk nutrients	523:552	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	1	50	theme	milk	242:245	arg1	production					247:256	milk production	242:256	milk production	242:256	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	9	51	theme	drying	1482:1487	arg1	factors					1516:1522	drying period-related non-genetic factors	1482:1522	drying period-related non-genetic factors	1482:1522	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	4	52	from	%	644:644	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	52	from	%	644:644	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	9	53	theme	non-genetic	1504:1514	arg1	factors					1516:1522	drying period-related non-genetic factors	1482:1522	drying period-related non-genetic factors	1482:1522	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	7	54	theme	cell	1270:1273	arg1	count					1275:1279	somatic cell count	1262:1279	somatic cell count	1262:1279	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	6	55	theme	composition	1091:1101	arg1	progression					1103:1113	composition progression	1091:1113	composition progression	1091:1113	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	3	56	from	effects	471:477	arg1	phenomics					509:517	the phenomics	505:517	the phenomics for quality-related milk nutrients and yield	505:562	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	2	57	from	goats	353:357	arg1	records					330:336	2594 milk records	320:336	2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed	320:415	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
36402938	6	58	theme	period	993:998	arg1	factors					1000:1006	Milking routine and drying period factors	966:1006	factors	1000:1006	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	58	theme	period	993:998	arg1	predictors					1022:1031	reference predictors	1012:1031	reference predictors	1012:1031	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	58	theme	period	993:998	arg1	routine					974:980	Milking routine and drying period factors	966:1006	routine	974:980	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	8	59	theme	milk	1394:1397	arg1	yield					1399:1403	milk yield	1394:1403	milk yield	1394:1403	Variability across drying years may depend on the drying season rather than the drying month course, except for milk yield, for which an increasing trend was reported from winter to summer.
36402938	2	60	theme	milk	325:328	arg1	records					330:336	2594 milk records	320:336	2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed	320:415	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
36402938	4	61	from	%	630:630	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	61	from	%	630:630	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	6	62	theme	milk	1071:1074	arg1	production					1076:1085	milk production	1071:1085	milk production	1071:1085	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	63	theme	drying	986:991	arg1	factors					1000:1006	Milking routine and drying period factors	966:1006	factors	1000:1006	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	63	theme	drying	986:991	arg1	predictors					1022:1031	reference predictors	1012:1031	reference predictors	1012:1031	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	6	63	theme	drying	986:991	arg1	routine					974:980	Milking routine and drying period factors	966:1006	routine	974:980	Milking routine and drying period factors are reference predictors to be considered in the evaluation of milk production and composition progression.
36402938	9	64	theme	regression	1576:1585	arg1	models					1587:1592	the regression models	1572:1592	the regression models	1572:1592	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	8	65	theme	month	1369:1373	arg1	course					1375:1380	the drying month course	1358:1380	the drying season rather than the drying month course	1328:1380	Variability across drying years may depend on the drying season rather than the drying month course, except for milk yield, for which an increasing trend was reported from winter to summer.
36402938	4	66	from	%	623:623	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	66	from	%	623:623	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	67	from	yield	750:754	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	67	from	yield	750:754	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	0	68	from	evaluation	9:18	arg1	phenomics					64:72	the phenomics	60:72	the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats	60:145	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	0	69	theme	milk	94:97	arg1	nutrients					99:107	quality-related milk nutrients	78:107	quality-related milk nutrients	78:107	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	8	70	theme	drying	1362:1367	arg1	course					1375:1380	the drying month course	1358:1380	the drying season rather than the drying month course	1328:1380	Variability across drying years may depend on the drying season rather than the drying month course, except for milk yield, for which an increasing trend was reported from winter to summer.
36402938	4	71	from	count	699:703	arg1	%					662:662	47.5%	658:662	47.5%	658:662	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	protein					732:738	protein	732:738	protein (%)	732:742	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	lactose					719:725	lactose	719:725	lactose (%)	719:729	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					787:787	%	787:787	%	787:787	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					630:630	40.0%	626:630	40.0%	626:630	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					728:728	%	728:728	%	728:728	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					741:741	%	741:741	%	741:741	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					644:644	44.3%	640:644	44.3%	640:644	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	kg					757:758	kg	757:758	kg	757:758	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					637:637	41.5%	633:637	41.5%	633:637	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					651:651	44.6%	647:651	44.6%	647:651	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					623:623	21.5%	619:623	21.5%	619:623	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	variability					671:681	the variability	667:681	the variability in somatic cell count (SCC, sc/mL)	667:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	fat					762:764	fat	762:764	fat (%)	762:768	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	matter					779:784	dry matter	775:784	dry matter (%)	775:788	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	%					767:767	%	767:767	%	767:767	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	71	from	count	699:703	arg1	yield					750:754	milk yield	745:754	milk yield (kg)	745:759	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	2	72	theme	breeding	368:375	arg1	nucleus					377:383	the breeding nucleus	364:383	the breeding nucleus of the Murciano-Granadina breed	364:415	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
36402938	9	73	dep	Including	1472:1480	arg1	permits					1598:1604	permits	1598:1604	permits to boost the commercial possibilities and profitability of local breeds	1598:1676	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	9	73	dep	Including	1472:1480	arg1	improves					1547:1554	improves	1547:1554	improves the accuracy of the regression models	1547:1592	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	4	74	from	protein	732:738	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	74	from	protein	732:738	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					662:662	47.5%	658:662	47.5%	658:662	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	protein					732:738	protein	732:738	protein (%)	732:742	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	lactose					719:725	lactose	719:725	lactose (%)	719:729	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					787:787	%	787:787	%	787:787	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					630:630	40.0%	626:630	40.0%	626:630	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					728:728	%	728:728	%	728:728	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					741:741	%	741:741	%	741:741	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					644:644	44.3%	640:644	44.3%	640:644	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	kg					757:758	kg	757:758	kg	757:758	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					637:637	41.5%	633:637	41.5%	633:637	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					651:651	44.6%	647:651	44.6%	647:651	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					623:623	21.5%	619:623	21.5%	619:623	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	variability					671:681	the variability	667:681	the variability in somatic cell count (SCC, sc/mL)	667:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	fat					762:764	fat	762:764	fat (%)	762:768	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	matter					779:784	dry matter	775:784	dry matter (%)	775:788	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	%					767:767	%	767:767	%	767:767	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	75	theme	variability	671:681	arg1	yield					750:754	milk yield	745:754	milk yield (kg)	745:759	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	3	76	theme	Bayesian	418:425	arg1	regression					434:443	Bayesian linear regression	418:443	Bayesian linear regression	418:443	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	1	77	theme	composition	262:272	arg1	variability					227:237	the variability	223:237	the variability of milk production and composition	223:272	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	4	78	from	variability	671:681	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	78	from	variability	671:681	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	7	79	theme	milk	1167:1170	arg1	yield					1172:1176	milk yield	1167:1176	milk yield	1167:1176	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	9	80	theme	local	1665:1669	arg1	breeds					1671:1676	local breeds	1665:1676	local breeds	1665:1676	Including drying period-related non-genetic factors in genetic evaluations improves the accuracy of the regression models and permits to boost the commercial possibilities and profitability of local breeds.
36402938	4	81	theme	Multivariate	565:576	arg1	model					589:593	Multivariate regression model	565:593	Multivariate regression model	565:593	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	82	theme	somatic	686:692	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	82	theme	somatic	686:692	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	2	83	theme	Murciano-Granadina	392:409	arg1	breed					411:415	the Murciano-Granadina breed	388:415	the Murciano-Granadina breed	388:415	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
36402938	1	84	theme	Bayesian	280:287	arg1	regression					296:305	Bayesian linear regression	280:305	Bayesian linear regression	280:305	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	5	85	theme	factor	833:838	arg1	combination					840:850	the aforementioned factor combination	814:850	the aforementioned factor combination	814:850	Although the aforementioned factor combination significantly conditions milk production and composition, SCC may be particularly affected by collateral factors.
36402938	0	86	theme	factors	49:55	arg1	effect					27:32	the effect	23:32	the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats	23:145	Bayesian evaluation of the effect of non-genetic factors on the phenomics for quality-related milk nutrients and yield in Murciano-Granadina goats.
36402938	3	87	theme	non-genetic	482:492	arg1	factors					494:500	non-genetic factors	482:500	non-genetic factors	482:500	Bayesian linear regression was used to determine the effects of non-genetic factors on the phenomics for quality-related milk nutrients and yield.
36402938	7	88	theme	dry	1237:1239	arg1	contents					1248:1255	dry matter contents	1237:1255	dry matter contents	1237:1255	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	7	89	theme	Drying	1116:1121	arg1	extensions					1130:1139	Drying period extensions	1116:1139	Drying period extensions	1116:1139	Drying period extensions positively repercussed on milk yield and lactose content, but negatively affected fat, protein, dry matter contents, and somatic cell count.
36402938	8	90	theme	increasing	1419:1428	arg1	trend					1430:1434	an increasing trend	1416:1434	an increasing trend	1416:1434	Variability across drying years may depend on the drying season rather than the drying month course, except for milk yield, for which an increasing trend was reported from winter to summer.
36402938	4	91	from	%	662:662	arg1	SCC					706:708	SCC	706:708	SCC	706:708	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	4	91	from	%	662:662	arg1	count					699:703	somatic cell count	686:703	somatic cell count (SCC, sc/mL)	686:716	Multivariate regression model significantly explained 21.5%, 40.0%, 41.5%, 44.3%, 44.6%, and 47.5% of the variability in somatic cell count (SCC, sc/mL), lactose (%), protein (%), milk yield (kg), fat (%), and dry matter (%), respectively.
36402938	1	92	theme	factors	212:218	arg1	effect					190:195	the effect	186:195	the effect of non-genetic factors on the variability of milk production and composition	186:272	The aim of this study was to evaluate the effect of non-genetic factors on the variability of milk production and composition using Bayesian linear regression.
36402938	2	93	theme	dairy	347:351	arg1	goats					353:357	159 dairy goats	343:357	159 dairy goats	343:357	We analyzed 2594 milk records from 159 dairy goats from the breeding nucleus of the Murciano-Granadina breed.
37160284	6	0	theme	Physiological	977:989	arg1	characterisation					991:1006	Physiological characterisation	977:1006	Physiological characterisation	977:1006	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	2	1	from	characteristics	438:452	arg1	resources					491:499	different germplasm resources	471:499	different germplasm resources combined with an analysis of the transcriptome	471:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	0	2	theme	shooting	87:94	arg1	shrivelling					96:106	shooting shrivelling	87:106	shooting shrivelling of apple branches overwinter	87:135	Transcriptome analysis reveals wax and phytohormone metabolism potentially involved in shooting shrivelling of apple branches overwinter.
37160284	5	3	theme	related	835:841	arg1	DEGs					830:833	DEGs	830:833	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis	830:946	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis were significantly enriched.
37160284	8	4	theme	Wax	1368:1370	arg1	content					1372:1378	Wax content	1368:1378	Wax content	1368:1378	Wax content, morphology and composition also exhibited difference.
37160284	6	5	theme	sugar	1079:1083	arg1	accumulation					1055:1066	the accumulation	1051:1066	the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'	1051:1250	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	0	6	theme	apple	111:115	arg1	branches					117:124	apple branches	111:124	apple branches overwinter	111:135	Transcriptome analysis reveals wax and phytohormone metabolism potentially involved in shooting shrivelling of apple branches overwinter.
37160284	1	7	from	development	186:196	arg1	hemisphere					233:242	the northern hemisphere	220:242	the northern hemisphere	220:242	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	6	8	dep	2.31times	1134:1142	arg1	that					1144:1147	that	1144:1147	that	1144:1147	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	6	9	theme	soluble	1071:1077	arg1	sugar					1079:1083	soluble sugar	1071:1083	soluble sugar	1071:1083	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	9	10	theme	trees	1639:1643	arg1	overwintering					1622:1634	normal overwintering	1615:1634	normal overwintering of trees	1615:1643	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	3	11	theme	initial	590:596	arg1	dormancy					598:605	the initial dormancy	586:605	the initial dormancy	586:605	Transcriptomes of samples treated in the initial dormancy, deep dormancy and freeze-thaw periods were generated and characterised.
37160284	0	12	dep	branches	117:124	arg1	overwinter					126:135	overwinter	126:135	overwinter	126:135	Transcriptome analysis reveals wax and phytohormone metabolism potentially involved in shooting shrivelling of apple branches overwinter.
37160284	1	13	theme	significant	278:288	arg1	difference					290:299	a significant difference	276:299	a significant difference in shoot shrivelling rate between different apple varieties in practice	276:371	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	2	14	from	resources	491:499	arg1	branches					457:464	branches	457:464	branches from different germplasm resources combined with an analysis of the transcriptome	457:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	2	14	from	resources	491:499	arg1	characteristics					438:452	the anatomical and physiological characteristics	405:452	the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome	405:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	5	15	theme	sucrose	892:898	arg1	metabolism					900:909	starch and sucrose metabolism	881:909	metabolism	900:909	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis were significantly enriched.
37160284	1	16	from	growth	175:180	arg1	hemisphere					233:242	the northern hemisphere	220:242	the northern hemisphere	220:242	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	6	17	theme	Delicious	1204:1212	arg1	content					1192:1198	the critical water content	1173:1198	the critical water content of 'Delicious'	1173:1213	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	6	17	theme	Delicious	1204:1212	arg1	higher					1233:1238	higher	1233:1238	higher	1233:1238	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	4	18	theme	expressed	728:736	arg1	DEGs					745:748	DEGs	745:748	DEGs	745:748	In three different periods, 7233 differentially expressed genes (DEGs) were identified including 3538 upregulated genes and 3695 downregulated genes.
37160284	4	18	theme	expressed	728:736	arg1	genes					738:742	7233 differentially expressed genes	708:742	7233 differentially expressed genes (DEGs)	708:749	In three different periods, 7233 differentially expressed genes (DEGs) were identified including 3538 upregulated genes and 3695 downregulated genes.
37160284	2	19	theme	anatomical	409:418	arg1	characteristics					438:452	the anatomical and physiological characteristics	405:452	the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome	405:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	0	20	theme	branches	117:124	arg1	shrivelling					96:106	shooting shrivelling	87:106	shooting shrivelling of apple branches overwinter	87:135	Transcriptome analysis reveals wax and phytohormone metabolism potentially involved in shooting shrivelling of apple branches overwinter.
37160284	5	21	theme	signal	860:865	arg1	transduction					867:878	plant hormone signal transduction	846:878	plant hormone signal transduction	846:878	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis were significantly enriched.
37160284	2	22	theme	different	471:479	arg1	resources					491:499	different germplasm resources	471:499	different germplasm resources combined with an analysis of the transcriptome	471:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	1	23	theme	deciduous	201:209	arg1	trees					211:215	deciduous trees	201:215	deciduous trees in the northern hemisphere	201:242	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	2	24	theme	germplasm	481:489	arg1	resources					491:499	different germplasm resources	471:499	different germplasm resources combined with an analysis of the transcriptome	471:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	1	25	from	difference	290:299	arg1	rate					322:325	shoot shrivelling rate	304:325	shoot shrivelling rate	304:325	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	3	26	theme	deep	608:611	arg1	dormancy					613:620	deep dormancy	608:620	deep dormancy	608:620	Transcriptomes of samples treated in the initial dormancy, deep dormancy and freeze-thaw periods were generated and characterised.
37160284	1	27	theme	trees	211:215	arg1	growth					175:180	growth	175:180	growth	175:180	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	1	27	theme	trees	211:215	arg1	development					186:196	development	186:196	development	186:196	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	0	28	theme	Transcriptome	0:12	arg1	analysis					14:21	Transcriptome analysis	0:21	Transcriptome analysis	0:21	Transcriptome analysis reveals wax and phytohormone metabolism potentially involved in shooting shrivelling of apple branches overwinter.
37160284	4	29	theme	upregulated	782:792	arg1	genes					794:798	3538 upregulated genes	777:798	3538 upregulated genes	777:798	In three different periods, 7233 differentially expressed genes (DEGs) were identified including 3538 upregulated genes and 3695 downregulated genes.
37160284	9	30	theme	branch	1450:1455	arg1	microstructure					1457:1470	branch microstructure	1450:1470	branch microstructure	1450:1470	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	6	31	theme	water	1186:1190	arg1	content					1192:1198	the critical water content	1173:1198	the critical water content of 'Delicious'	1173:1213	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	6	31	theme	water	1186:1190	arg1	higher					1233:1238	higher	1233:1238	higher	1233:1238	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	6	32	theme	Fuji	1124:1127	arg1	rate					1115:1118	shoot shrivelling rate	1097:1118	shoot shrivelling rate of 'Fuji'	1097:1128	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	2	33	theme	branches	457:464	arg1	characteristics					438:452	the anatomical and physiological characteristics	405:452	the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome	405:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	1	34	theme	shoot	304:308	arg1	rate					322:325	shoot shrivelling rate	304:325	shoot shrivelling rate	304:325	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	9	35	dep	trees	1553:1557	arg1	regulate					1581:1588	regulate	1581:1588	can regulate wax metabolism to ensure normal overwintering of trees	1577:1643	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	6	36	theme	critical	1177:1184	arg1	content					1192:1198	the critical water content	1173:1198	the critical water content of 'Delicious'	1173:1213	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	6	36	theme	critical	1177:1184	arg1	higher					1233:1238	higher	1233:1238	higher	1233:1238	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	1	37	theme	northern	224:231	arg1	hemisphere					233:242	the northern hemisphere	220:242	the northern hemisphere	220:242	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	0	38	theme	phytohormone	39:50	arg1	metabolism					52:61	phytohormone metabolism	39:61	phytohormone metabolism	39:61	Transcriptome analysis reveals wax and phytohormone metabolism potentially involved in shooting shrivelling of apple branches overwinter.
37160284	1	39	theme	shrivelling	310:320	arg1	rate					322:325	shoot shrivelling rate	304:325	shoot shrivelling rate	304:325	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	7	40	theme	overwintering	1327:1339	arg1	phase					1341:1345	the overwintering phase	1323:1345	the overwintering phase among two varieties	1323:1365	Phytohormone contents and proportions varied irregularly according to the overwintering phase among two varieties.
37160284	2	41	theme	physiological	424:436	arg1	characteristics					438:452	the anatomical and physiological characteristics	405:452	the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome	405:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	9	42	theme	wax	1490:1492	arg1	metabolism					1494:1503	wax metabolism	1490:1503	wax metabolism	1490:1503	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	4	43	theme	downregulated	809:821	arg1	genes					823:827	3695 downregulated genes	804:827	3695 downregulated genes	804:827	In three different periods, 7233 differentially expressed genes (DEGs) were identified including 3538 upregulated genes and 3695 downregulated genes.
37160284	1	44	from	hemisphere	233:242	arg1	growth					175:180	growth	175:180	growth	175:180	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	1	44	from	hemisphere	233:242	arg1	development					186:196	development	186:196	development	186:196	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	4	45	theme	different	689:697	arg1	periods					699:705	three different periods	683:705	three different periods	683:705	In three different periods, 7233 differentially expressed genes (DEGs) were identified including 3538 upregulated genes and 3695 downregulated genes.
37160284	1	46	from	varieties	351:359	arg1	practice					364:371	practice	364:371	practice	364:371	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	6	47	theme	dormancy	1020:1027	arg1	overwinter					1029:1038	dormancy overwinter	1020:1038	dormancy overwinter	1020:1038	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	7	48	theme	Phytohormone	1253:1264	arg1	contents					1266:1273	Phytohormone contents	1253:1273	Phytohormone contents	1253:1273	Phytohormone contents and proportions varied irregularly according to the overwintering phase among two varieties.
37160284	9	49	theme	trees	1553:1557	arg1	performance					1538:1548	the overwintering performance	1520:1548	the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees	1520:1643	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	5	50	theme	hormone	852:858	arg1	transduction					867:878	plant hormone signal transduction	846:878	plant hormone signal transduction	846:878	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis were significantly enriched.
37160284	1	51	theme	different	335:343	arg1	varieties					351:359	different apple varieties	335:359	different apple varieties in practice	335:371	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	3	52	theme	freeze-thaw	626:636	arg1	periods					638:644	freeze-thaw periods	626:644	freeze-thaw periods	626:644	Transcriptomes of samples treated in the initial dormancy, deep dormancy and freeze-thaw periods were generated and characterised.
37160284	9	53	theme	overwintering	1524:1536	arg1	performance					1538:1548	the overwintering performance	1520:1548	the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees	1520:1643	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	1	54	theme	Shoot	138:142	arg1	shrivelling					144:154	Shoot shrivelling	138:154	Shoot shrivelling	138:154	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	1	55	theme	apple	345:349	arg1	varieties					351:359	different apple varieties	335:359	different apple varieties in practice	335:371	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	9	56	theme	phytohormones	1563:1575	arg1	performance					1538:1548	the overwintering performance	1520:1548	the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees	1520:1643	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	6	57	theme	shrivelling	1103:1113	arg1	rate					1115:1118	shoot shrivelling rate	1097:1118	shoot shrivelling rate of 'Fuji'	1097:1128	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	1	58	from	trees	211:215	arg1	hemisphere					233:242	the northern hemisphere	220:242	the northern hemisphere	220:242	Shoot shrivelling severely threatens growth and development of deciduous trees in the northern hemisphere, and we observed that there was a significant difference in shoot shrivelling rate between different apple varieties in practice.
37160284	9	59	theme	wax	1590:1592	arg1	metabolism					1594:1603	wax metabolism	1590:1603	wax metabolism	1590:1603	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	6	60	theme	shoot	1097:1101	arg1	rate					1115:1118	shoot shrivelling rate	1097:1118	shoot shrivelling rate of 'Fuji'	1097:1128	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	2	61	theme	transcriptome	534:546	arg1	analysis					518:525	an analysis	515:525	an analysis of the transcriptome	515:546	In this study, we investigated the anatomical and physiological characteristics of branches from different germplasm resources combined with an analysis of the transcriptome.
37160284	7	62	dep	varied	1291:1296	arg1	according					1310:1318	according	1310:1318	varied irregularly according to the overwintering phase among two varieties	1291:1365	Phytohormone contents and proportions varied irregularly according to the overwintering phase among two varieties.
37160284	5	63	theme	wax	931:933	arg1	biosynthesis					935:946	wax biosynthesis	931:946	wax biosynthesis	931:946	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis were significantly enriched.
37160284	6	64	theme	2.31times	1134:1142	arg1	accumulation					1055:1066	the accumulation	1051:1066	the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'	1051:1250	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
37160284	9	65	theme	normal	1615:1620	arg1	overwintering					1622:1634	normal overwintering	1615:1634	normal overwintering of trees	1615:1643	In conclusion, branch microstructure, phytohormone and wax metabolism all determined the overwintering performance of trees and phytohormones can regulate wax metabolism to ensure normal overwintering of trees.
37160284	5	66	theme	plant	846:850	arg1	transduction					867:878	plant hormone signal transduction	846:878	plant hormone signal transduction	846:878	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis were significantly enriched.
37160284	5	67	theme	starch	881:886	arg1	metabolism					900:909	starch and sucrose metabolism	881:909	metabolism	900:909	DEGs related to plant hormone signal transduction, starch and sucrose metabolism, cutin, suberin and wax biosynthesis were significantly enriched.
37160284	3	68	theme	samples	567:573	arg1	Transcriptomes					549:562	Transcriptomes	549:562	Transcriptomes of samples treated in the initial dormancy, deep dormancy and freeze-thaw periods	549:644	Transcriptomes of samples treated in the initial dormancy, deep dormancy and freeze-thaw periods were generated and characterised.
37160284	6	69	theme	starch	1089:1094	arg1	accumulation					1055:1066	the accumulation	1051:1066	the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'	1051:1250	Physiological characterisation showed that dormancy overwinter can induce the accumulation of soluble sugar and starch, shoot shrivelling rate of 'Fuji' was 2.31times that of the 'Delicious'; and the critical water content of 'Delicious' was significantly higher than 'Fuji'.
35380003	1	0	theme	internal	145:152	arg1	freezing					154:161	internal freezing	145:161	internal freezing	145:161	Insects that naturally tolerate internal freezing produce complex mixtures of multiple cryoprotectants (CPs).
35380003	9	1	theme	amorphous	1725:1733	arg1	liquids					1758:1764	amorphous glass and viscoelastic liquids	1725:1764	amorphous glass and viscoelastic liquids	1725:1764	We propose that amorphous glass and viscoelastic liquids may protect macromolecules and cells from thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen.
35380003	5	2	theme	artificial	908:917	arg1	mixtures					919:926	artificial mixtures	908:926	artificial mixtures	908:926	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	3	3	theme	resolution	497:506	arg1	spectrometry					513:524	high resolution mass spectrometry	492:524	high resolution mass spectrometry	492:524	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	5	4	from	hemolymph	974:982	arg1	concentrations					943:956	the concentrations	939:956	the concentrations of major CPs in hemolymph of freeze-tolerant larvae	939:1008	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	8	5	theme	liquid	1701:1706	arg1	layer					1673:1677	a layer	1671:1677	a layer of dense viscoelastic liquid	1671:1706	In contrast, proline moves to the boundary between extracellular ice and dehydrated hemolymph and tissues where it probably forms a layer of dense viscoelastic liquid.
35380003	9	6	theme	glass	1735:1739	arg1	liquids					1758:1764	amorphous glass and viscoelastic liquids	1725:1764	amorphous glass and viscoelastic liquids	1725:1764	We propose that amorphous glass and viscoelastic liquids may protect macromolecules and cells from thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen.
35380003	3	7	theme	mass	508:511	arg1	spectrometry					513:524	high resolution mass spectrometry	492:524	high resolution mass spectrometry	492:524	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	4	8	dep	CPs	674:676	arg1	sarcosine					792:800	sarcosine	792:800	sarcosine	792:800	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	trehalose					687:695	trehalose	687:695	trehalose	687:695	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	betaine					729:735	glycine betaine	721:735	glycine betaine	721:735	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	glutamine					698:706	glutamine	698:706	glutamine	698:706	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	glycerophosphoethanolamine					738:763	glycerophosphoethanolamine	738:763	glycerophosphoethanolamine	738:763	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	CPs					674:676	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine	670:800	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine	670:800	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	proline					678:684	proline	678:684	proline	678:684	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	asparagine					709:718	asparagine	709:718	asparagine	709:718	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	8	dep	CPs	674:676	arg1	glycerophosphocholine					766:786	glycerophosphocholine	766:786	glycerophosphocholine	766:786	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	7	9	theme	matrix-assisted	1284:1298	arg1	spectrometry					1333:1344	matrix-assisted laser desorption/ionization mass spectrometry	1284:1344	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	1284:1364	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	3	10	theme	high	492:495	arg1	spectrometry					513:524	high resolution mass spectrometry	492:524	high resolution mass spectrometry	492:524	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	7	11	theme	spectrometry	1333:1344	arg1	MALDI-MSI					1355:1363	MALDI-MSI	1355:1363	MALDI-MSI	1355:1363	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	7	11	theme	spectrometry	1333:1344	arg1	imaging					1346:1352	matrix-assisted laser desorption/ionization mass spectrometry imaging	1284:1352	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	1284:1364	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	0	12	theme	fly	102:104	arg1	larva					106:110	a drosophilid fly larva	88:110	a drosophilid fly larva	88:110	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	7	13	theme	laser	1300:1304	arg1	spectrometry					1333:1344	matrix-assisted laser desorption/ionization mass spectrometry	1284:1344	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	1284:1364	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	5	14	theme	major	961:965	arg1	CPs					967:969	major CPs	961:969	major CPs in hemolymph of freeze-tolerant larvae	961:1008	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	2	15	theme	cells	417:421	arg1	cryopreservation					397:412	cryopreservation	397:412	cryopreservation of cells and other biological material	397:451	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	5	16	theme	freeze-tolerant	987:1001	arg1	larvae					1003:1008	freeze-tolerant larvae	987:1008	freeze-tolerant larvae	987:1008	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	0	17	theme	drosophilid	90:100	arg1	larva					106:110	a drosophilid fly larva	88:110	a drosophilid fly larva	88:110	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	7	18	theme	glass	1528:1532	arg1	phase					1534:1538	the amorphous glass phase	1514:1538	the amorphous glass phase	1514:1538	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	2	19	theme	mixtures	264:271	arg1	composition					243:253	composition	243:253	composition of these mixtures	243:271	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	2	20	from	knowledge	230:238	arg1	composition					243:253	composition	243:253	composition of these mixtures	243:271	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	2	20	from	knowledge	230:238	arg1	mechanisms					285:294	the mechanisms	281:294	the mechanisms of individual CP interactions	281:324	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	1	21	theme	complex	171:177	arg1	mixtures					179:186	complex mixtures	171:186	complex mixtures of multiple cryoprotectants (CPs)	171:220	Insects that naturally tolerate internal freezing produce complex mixtures of multiple cryoprotectants (CPs).
35380003	5	22	from	concentrations	943:956	arg1	hemolymph					974:982	hemolymph	974:982	hemolymph of freeze-tolerant larvae	974:1008	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	2	23	theme	individual	299:308	arg1	interactions					313:324	individual CP interactions	299:324	individual CP interactions	299:324	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	8	24	theme	extracellular	1592:1604	arg1	ice					1606:1608	extracellular ice and dehydrated hemolymph	1592:1633	ice	1606:1608	In contrast, proline moves to the boundary between extracellular ice and dehydrated hemolymph and tissues where it probably forms a layer of dense viscoelastic liquid.
35380003	2	25	theme	formulations	370:381	arg1	development					341:351	development	341:351	development of laboratory CP formulations optimized for cryopreservation of cells and other biological material	341:451	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	2	26	theme	material	444:451	arg1	cryopreservation					397:412	cryopreservation	397:412	cryopreservation of cells and other biological material	397:451	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	4	27	theme	313:108:55:26:6:4:2.9:0.5 mmol l-1	840:873	arg1	ratio					831:835	a ratio	829:835	a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1	829:873	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	3	28	theme	larval	554:559	arg1	tissues					561:567	larval tissues	554:567	larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen	554:667	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	7	29	from	concentrated	1433:1444	arg1	hemolymph					1470:1478	partially dehydrated hemolymph	1449:1478	partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase	1449:1538	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	2	30	theme	CP	367:368	arg1	formulations					370:381	laboratory CP formulations	356:381	laboratory CP formulations optimized for cryopreservation of cells and other biological material	356:451	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	0	31	theme	innate	13:18	arg1	cryoprotectants					20:34	innate cryoprotectants	13:34	innate cryoprotectants	13:34	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	7	32	theme	extracellular	1392:1404	arg1	freezing					1406:1413	slow extracellular freezing	1387:1413	slow extracellular freezing	1387:1413	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	3	33	theme	putative	538:545	arg1	CPs					547:549	putative CPs	538:549	putative CPs	538:549	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	1	34	theme	multiple	191:198	arg1	cryoprotectants					200:214	multiple cryoprotectants	191:214	multiple cryoprotectants (CPs)	191:220	Insects that naturally tolerate internal freezing produce complex mixtures of multiple cryoprotectants (CPs).
35380003	1	34	theme	multiple	191:198	arg1	CPs					217:219	CPs	217:219	CPs	217:219	Insects that naturally tolerate internal freezing produce complex mixtures of multiple cryoprotectants (CPs).
35380003	2	35	theme	other	427:431	arg1	material					444:451	other biological material	427:451	other biological material	427:451	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	0	36	theme	larva	106:110	arg1	cryopreservation					68:83	cryopreservation	68:83	cryopreservation of a drosophilid fly larva	68:110	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	0	36	theme	larva	106:110	arg1	tolerance					54:62	freeze tolerance	47:62	freeze tolerance	47:62	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	5	37	theme	CPs	967:969	arg1	concentrations					943:956	the concentrations	939:956	the concentrations of major CPs in hemolymph of freeze-tolerant larvae	939:1008	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	3	38	theme	CPs	547:549	arg1	range					529:533	a range	527:533	a range of putative CPs	527:549	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	1	39	theme	cryoprotectants	200:214	arg1	mixtures					179:186	complex mixtures	171:186	complex mixtures of multiple cryoprotectants (CPs)	171:220	Insects that naturally tolerate internal freezing produce complex mixtures of multiple cryoprotectants (CPs).
35380003	6	40	theme	freeze-sensitive	1253:1268	arg1	larvae					1270:1275	otherwise freeze-sensitive larvae	1243:1275	otherwise freeze-sensitive larvae	1243:1275	We demonstrate in a bioassay that mixtures of CPs administered through the diet act synergistically rather than additively to enable cryopreservation of otherwise freeze-sensitive larvae.
35380003	3	41	theme	long-term	623:631	arg1	cryopreservation					633:648	long-term cryopreservation	623:648	long-term cryopreservation in liquid nitrogen	623:667	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	7	42	theme	amorphous	1518:1526	arg1	phase					1534:1538	the amorphous glass phase	1514:1538	the amorphous glass phase	1514:1538	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	0	43	theme	cryoprotectants	20:34	arg1	key					39:41	key	39:41	key	39:41	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	0	43	theme	cryoprotectants	20:34	arg1	mixture					2:8	A mixture	0:8	A mixture of innate cryoprotectants	0:34	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	2	44	theme	Better	223:228	arg1	knowledge					230:238	Better knowledge	223:238	Better knowledge	223:238	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	9	45	theme	liquid	1886:1891	arg1	nitrogen					1893:1900	liquid nitrogen	1886:1900	liquid nitrogen	1886:1900	We propose that amorphous glass and viscoelastic liquids may protect macromolecules and cells from thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen.
35380003	9	46	theme	thermomechanical	1808:1823	arg1	shocks					1825:1830	thermomechanical shocks	1808:1830	thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen	1808:1900	We propose that amorphous glass and viscoelastic liquids may protect macromolecules and cells from thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen.
35380003	5	47	theme	water	1041:1045	arg1	point					1032:1036	the melting point	1020:1036	the melting point of water	1020:1045	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	8	48	theme	dehydrated	1614:1623	arg1	hemolymph					1625:1633	extracellular ice and dehydrated hemolymph	1592:1633	hemolymph	1625:1633	In contrast, proline moves to the boundary between extracellular ice and dehydrated hemolymph and tissues where it probably forms a layer of dense viscoelastic liquid.
35380003	2	49	theme	biological	433:442	arg1	material					444:451	other biological material	427:451	other biological material	427:451	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	6	50	theme	larvae	1270:1275	arg1	cryopreservation					1223:1238	cryopreservation	1223:1238	cryopreservation of otherwise freeze-sensitive larvae	1223:1275	We demonstrate in a bioassay that mixtures of CPs administered through the diet act synergistically rather than additively to enable cryopreservation of otherwise freeze-sensitive larvae.
35380003	3	51	theme	liquid	653:658	arg1	nitrogen					660:667	liquid nitrogen	653:667	liquid nitrogen	653:667	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	6	52	theme	CPs	1136:1138	arg1	mixtures					1124:1131	mixtures	1124:1131	mixtures of CPs administered through the diet	1124:1168	We demonstrate in a bioassay that mixtures of CPs administered through the diet act synergistically rather than additively to enable cryopreservation of otherwise freeze-sensitive larvae.
35380003	5	53	from	CPs	967:969	arg1	hemolymph					974:982	hemolymph	974:982	hemolymph of freeze-tolerant larvae	974:1008	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	3	54	theme	Chymomyza	589:597	arg1	fly					584:586	a subarctic fly	572:586	a subarctic fly	572:586	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	3	54	theme	Chymomyza	589:597	arg1	costata					599:605	Chymomyza costata	589:605	Chymomyza costata	589:605	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	3	55	theme	subarctic	574:582	arg1	fly					584:586	a subarctic fly	572:586	a subarctic fly	572:586	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	3	55	theme	subarctic	574:582	arg1	costata					599:605	Chymomyza costata	589:605	Chymomyza costata	589:605	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	2	56	theme	interactions	313:324	arg1	mechanisms					285:294	the mechanisms	281:294	the mechanisms of individual CP interactions	281:324	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	4	57	theme	glycine	721:727	arg1	CPs					674:676	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine	670:800	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine	670:800	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	4	57	theme	glycine	721:727	arg1	betaine					729:735	glycine betaine	721:735	glycine betaine	721:735	The CPs proline, trehalose, glutamine, asparagine, glycine betaine, glycerophosphoethanolamine, glycerophosphocholine and sarcosine accumulate in hemolymph in a ratio of 313:108:55:26:6:4:2.9:0.5 mmol l-1.
35380003	5	58	theme	larvae	1003:1008	arg1	hemolymph					974:982	hemolymph	974:982	hemolymph of freeze-tolerant larvae	974:1008	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	2	59	theme	CP	310:311	arg1	interactions					313:324	individual CP interactions	299:324	individual CP interactions	299:324	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	9	60	dep	nitrogen	1893:1900	arg1	out					1879:1881	out	1879:1881	out	1879:1881	We propose that amorphous glass and viscoelastic liquids may protect macromolecules and cells from thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen.
35380003	7	61	theme	mass	1328:1331	arg1	spectrometry					1333:1344	matrix-assisted laser desorption/ionization mass spectrometry	1284:1344	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	1284:1364	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	3	62	from	cryopreservation	633:648	arg1	nitrogen					660:667	liquid nitrogen	653:667	liquid nitrogen	653:667	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	5	63	theme	ice	1076:1078	arg1	fraction					1080:1087	the ice fraction	1072:1087	the ice fraction	1072:1087	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	3	64	theme	fly	584:586	arg1	tissues					561:567	larval tissues	554:567	larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen	554:667	Here, we identify and quantify (using high resolution mass spectrometry) a range of putative CPs in larval tissues of a subarctic fly, Chymomyza costata, which survives long-term cryopreservation in liquid nitrogen.
35380003	2	65	theme	laboratory	356:365	arg1	formulations					370:381	laboratory CP formulations	356:381	laboratory CP formulations optimized for cryopreservation of cells and other biological material	356:451	Better knowledge on composition of these mixtures, and on the mechanisms of individual CP interactions, could inspire development of laboratory CP formulations optimized for cryopreservation of cells and other biological material.
35380003	7	66	theme	slow	1387:1390	arg1	freezing					1406:1413	slow extracellular freezing	1387:1413	slow extracellular freezing	1387:1413	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	0	67	theme	freeze	47:52	arg1	tolerance					54:62	freeze tolerance	47:62	freeze tolerance	47:62	A mixture of innate cryoprotectants is key for freeze tolerance and cryopreservation of a drosophilid fly larva.
35380003	9	68	theme	viscoelastic	1745:1756	arg1	liquids					1758:1764	amorphous glass and viscoelastic liquids	1725:1764	amorphous glass and viscoelastic liquids	1725:1764	We propose that amorphous glass and viscoelastic liquids may protect macromolecules and cells from thermomechanical shocks associated with freezing and transfer into and out of liquid nitrogen.
35380003	5	69	theme	melting	1024:1030	arg1	point					1032:1036	the melting point	1020:1036	the melting point of water	1020:1045	Using calorimetry, we show that artificial mixtures, mimicking the concentrations of major CPs in hemolymph of freeze-tolerant larvae, suppress the melting point of water and significantly reduce the ice fraction.
35380003	7	70	from	hemolymph	1470:1478	arg1	concentrated					1433:1444	concentrated	1433:1444	concentrated	1433:1444	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	7	71	theme	desorption/ionization	1306:1326	arg1	spectrometry					1333:1344	matrix-assisted laser desorption/ionization mass spectrometry	1284:1344	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	1284:1364	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35380003	7	72	theme	dehydrated	1459:1468	arg1	hemolymph					1470:1478	partially dehydrated hemolymph	1449:1478	partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase	1449:1538	Using matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI), we show that during slow extracellular freezing trehalose becomes concentrated in partially dehydrated hemolymph where it stimulates transition to the amorphous glass phase.
35253976	3	0	theme	healthy	528:534	arg1	donors					542:547	healthy human donors	528:547	healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin	528:607	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	1	1	theme	processing	256:265	arg1	methods					267:273	divergent blood processing methods	240:273	divergent blood processing methods	240:273	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	3	2	theme	multiplexed	745:755	arg1	labeling					779:786	multiplexed tandem mass tag (TMT) labeling	745:786	multiplexed tandem mass tag (TMT) labeling	745:786	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	8	3	from	proteins	1536:1543	arg1	platelets					1548:1556	platelets	1548:1556	platelets from EDTA-anticoagulated blood	1548:1587	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	8	3	from	proteins	1536:1543	arg1	blood					1583:1587	EDTA-anticoagulated blood	1563:1587	EDTA-anticoagulated blood	1563:1587	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	3	4	theme	sodium	554:559	arg1	citrate					561:567	sodium citrate	554:567	sodium citrate	554:567	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	4	5	theme	time-dependent	865:878	arg1	effects					880:886	time-dependent effects	865:886	time-dependent effects	865:886	RESULTS Each anticoagulant had time-dependent effects on platelet function in whole blood.
35253976	9	6	theme	extracellular	1730:1742	arg1	formation					1755:1763	extracellular trap (NET) formation	1730:1763	extracellular trap (NET) formation	1730:1763	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	3	7	theme	mass	764:767	arg1	labeling					779:786	multiplexed tandem mass tag (TMT) labeling	745:786	multiplexed tandem mass tag (TMT) labeling	745:786	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	8	theme	citrate	575:581	arg1	dextrose					583:590	acid citrate dextrose	570:590	acid citrate dextrose	570:590	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	1	9	theme	hematology	203:212	arg1	studies					214:220	mechanistic and clinical hematology studies	178:220	mechanistic and clinical hematology studies	178:220	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	7	10	from	abundant	1265:1272	arg1	platelets					1277:1285	platelets	1277:1285	platelets	1277:1285	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	7	10	from	abundant	1265:1272	arg1	proteins					1363:1370	proteins	1363:1370	proteins pertinent to membrane trafficking and exocytosis	1363:1419	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	9	11	theme	NET	1778:1780	arg1	interactions					1782:1793	NET interactions	1778:1793	NET interactions in whole blood ex vivo	1778:1816	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	4	12	theme	whole	912:916	arg1	blood					918:922	whole blood	912:922	whole blood	912:922	RESULTS Each anticoagulant had time-dependent effects on platelet function in whole blood.
35253976	7	13	from	platelets	1277:1285	arg1	abundant					1265:1272	abundant	1265:1272	abundant	1265:1272	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	4	14	contain	had	861:863	arg1	anticoagulant					847:859	Each anticoagulant	842:859	Each anticoagulant	842:859	RESULTS Each anticoagulant had time-dependent effects on platelet function in whole blood.
35253976	4	14	contain	had	861:863	arg2	effects					880:886	time-dependent effects	865:886	time-dependent effects	865:886	RESULTS Each anticoagulant had time-dependent effects on platelet function in whole blood.
35253976	10	15	dep	ex	1954:1955	arg1	vivo					1957:1960	vivo	1957:1960	vivo	1957:1960	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	9	16	theme	trap	1744:1747	arg1	formation					1755:1763	extracellular trap (NET) formation	1730:1763	extracellular trap (NET) formation	1730:1763	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	1	17	from	effects	229:235	arg1	composition					287:297	platelet composition	278:297	platelet composition	278:297	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	5	18	theme	α-granule	1097:1105	arg1	secretion					1107:1115	platelet α-granule secretion	1088:1115	platelet α-granule secretion	1088:1115	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	10	19	theme	platelet	2007:2014	arg1	proteome					2016:2023	platelet proteome	2007:2023	platelet proteome	2007:2023	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	9	20	theme	histone	1653:1659	arg1	levels					1643:1648	higher levels	1636:1648	higher levels of histone and neutrophil-associated proteins	1636:1694	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	3	21	theme	quadrupole	799:808	arg1	spectrometry					815:826	triple quadrupole mass spectrometry	792:826	triple quadrupole mass spectrometry (MS)	792:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	21	theme	quadrupole	799:808	arg1	MS					829:830	MS	829:830	MS	829:830	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	0	22	theme	processing	59:68	arg1	time					70:73	preanalytical processing time	45:73	preanalytical processing time	45:73	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	4	23	from	function	900:907	arg1	blood					918:922	whole blood	912:922	whole blood	912:922	RESULTS Each anticoagulant had time-dependent effects on platelet function in whole blood.
35253976	9	24	theme	neutrophil-associated	1665:1685	arg1	proteins					1687:1694	neutrophil-associated proteins	1665:1694	neutrophil-associated proteins	1665:1694	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	10	25	contain	have	1920:1923	arg2	effects					1933:1939	varying effects	1925:1939	varying effects	1925:1939	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	10	25	contain	have	1920:1923	arg1	anticoagulants					1869:1882	different anticoagulants	1859:1882	different anticoagulants routinely used for blood collection	1859:1918	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	7	26	attach	isolated	1287:1294	arg2	proteins					1363:1370	proteins	1363:1370	proteins pertinent to membrane trafficking and exocytosis	1363:1419	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	7	26	attach	isolated	1287:1294	arg1	blood					1301:1305	blood	1301:1305	blood processed at 24 h versus 1 h post-phlebotomy	1301:1350	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	7	26	attach	isolated	1287:1294	arg2	platelets					1277:1285	platelets	1277:1285	platelets	1277:1285	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	8	27	theme	Anticoagulant-specific	1422:1443	arg1	effects					1445:1451	Anticoagulant-specific effects	1422:1451	Anticoagulant-specific effects on platelet proteomes	1422:1473	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	8	28	from	blood	1583:1587	arg1	platelets					1548:1556	platelets	1548:1556	platelets from EDTA-anticoagulated blood	1548:1587	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	8	28	from	blood	1583:1587	arg1	proteins					1536:1543	α-granule proteins	1526:1543	α-granule proteins in platelets from EDTA-anticoagulated blood	1526:1587	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	1	29	theme	function	151:158	arg1	assays					132:137	Ex vivo assays	124:137	Ex vivo assays of platelet function	124:158	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	0	30	from	Effects	0:6	arg1	content					91:97	the proteome content	78:97	the proteome content of platelets	78:110	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	1	31	dep	Ex	124:125	arg1	vivo					127:130	vivo	127:130	vivo	127:130	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	0	32	theme	proteome	82:89	arg1	content					91:97	the proteome content	78:97	the proteome content of platelets	78:110	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	5	33	theme	platelet-monocyte	985:1001	arg1	formation					1013:1021	platelet-monocyte aggregate formation	985:1021	platelet-monocyte aggregate formation	985:1021	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	9	34	contain	had	1632:1634	arg1	Platelets					1590:1598	Platelets	1590:1598	Platelets prepared from heparinized blood	1590:1630	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	9	34	contain	had	1632:1634	arg2	levels					1643:1648	higher levels	1636:1648	higher levels of histone and neutrophil-associated proteins	1636:1694	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	3	35	theme	Parallel	485:492	arg1	samples					500:506	Parallel blood samples	485:506	Parallel blood samples	485:506	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	2	36	theme	processing	406:415	arg1	times					417:421	processing times	406:421	processing times	406:421	OBJECTIVE Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.
35253976	6	37	theme	platelet	1128:1135	arg1	isolation					1137:1145	platelet isolation	1128:1145	platelet isolation	1128:1145	Following platelet isolation, TMT-MS quantified 3357 proteins amongst all prepared platelet samples.
35253976	8	38	theme	increased	1484:1492	arg1	system					1505:1510	increased complement system	1484:1510	increased complement system	1484:1510	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	1	39	theme	platelet	278:285	arg1	composition					287:297	platelet composition	278:297	platelet composition	278:297	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	5	40	theme	platelet	956:963	arg1	reactivity					973:982	platelet agonist reactivity	956:982	platelet agonist reactivity	956:982	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	2	41	theme	protein	452:458	arg1	content					460:466	protein content	452:466	protein content	452:466	OBJECTIVE Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.
35253976	0	42	theme	ex	11:12	arg1	anticoagulation					25:39	ex vivo blood anticoagulation	11:39	ex vivo blood anticoagulation	11:39	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	0	43	theme	blood	19:23	arg1	anticoagulation					25:39	ex vivo blood anticoagulation	11:39	ex vivo blood anticoagulation	11:39	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	3	44	theme	time	641:644	arg1	course					646:651	an extended time course	629:651	an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS)	629:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	10	45	theme	different	1859:1867	arg1	anticoagulants					1869:1882	different anticoagulants	1859:1882	different anticoagulants routinely used for blood collection	1859:1918	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	10	46	theme	biomarker	2070:2078	arg1	studies					2080:2086	biomarker studies	2070:2086	biomarker studies	2070:2086	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	3	47	theme	blood	494:498	arg1	samples					500:506	Parallel blood samples	485:506	Parallel blood samples	485:506	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	10	48	theme	blood	1903:1907	arg1	collection					1909:1918	blood collection	1903:1918	blood collection	1903:1918	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	5	49	theme	platelet	1027:1034	arg1	release					1058:1064	platelet extracellular vesicle release	1027:1064	platelet extracellular vesicle release	1027:1064	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	8	50	theme	EDTA-anticoagulated	1563:1581	arg1	blood					1583:1587	EDTA-anticoagulated blood	1563:1587	EDTA-anticoagulated blood	1563:1587	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	3	51	theme	human	536:540	arg1	donors					542:547	healthy human donors	528:547	healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin	528:607	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	8	52	from	effects	1445:1451	arg1	proteomes					1465:1473	platelet proteomes	1456:1473	platelet proteomes	1456:1473	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	5	53	theme	vesicle	1050:1056	arg1	release					1058:1064	platelet extracellular vesicle release	1027:1064	platelet extracellular vesicle release	1027:1064	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	3	54	theme	proteome	716:723	arg1	quantification					725:738	platelet proteome quantification	707:738	platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS)	707:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	1	55	theme	blood	250:254	arg1	methods					267:273	divergent blood processing methods	240:273	divergent blood processing methods	240:273	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	9	56	theme	whole	1798:1802	arg1	blood					1804:1808	whole blood	1798:1808	whole blood	1798:1808	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	9	57	dep	neutrophil	1719:1728	arg1	formation					1755:1763	extracellular trap (NET) formation	1730:1763	extracellular trap (NET) formation	1730:1763	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	1	58	theme	methods	267:273	arg1	effects					229:235	effects	229:235	effects of divergent blood processing methods on platelet composition	229:297	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	4	59	theme	platelet	891:898	arg1	function					900:907	platelet function	891:907	platelet function in whole blood	891:922	RESULTS Each anticoagulant had time-dependent effects on platelet function in whole blood.
35253976	9	60	theme	proteins	1687:1694	arg1	levels					1643:1648	higher levels	1636:1648	higher levels of histone and neutrophil-associated proteins	1636:1694	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	3	61	theme	tandem	757:762	arg1	labeling					779:786	multiplexed tandem mass tag (TMT) labeling	745:786	multiplexed tandem mass tag (TMT) labeling	745:786	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	10	62	theme	varying	1925:1931	arg1	effects					1933:1939	varying effects	1925:1939	varying effects	1925:1939	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	10	63	theme	ex	1954:1955	arg1	platelets					1944:1952	platelets	1944:1952	platelets ex vivo	1944:1960	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	1	64	theme	mechanistic	178:188	arg1	studies					214:220	mechanistic and clinical hematology studies	178:220	mechanistic and clinical hematology studies	178:220	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	3	65	theme	TMT	774:776	arg1	labeling					779:786	multiplexed tandem mass tag (TMT) labeling	745:786	multiplexed tandem mass tag (TMT) labeling	745:786	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	10	66	dep	CONCLUSION	1819:1828	arg1	demonstrate					1842:1852	demonstrate	1842:1852	demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies	1842:2086	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	3	67	theme	acid	570:573	arg1	dextrose					583:590	acid citrate dextrose	570:590	acid citrate dextrose	570:590	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	1	68	theme	clinical	194:201	arg1	studies					214:220	mechanistic and clinical hematology studies	178:220	mechanistic and clinical hematology studies	178:220	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	2	69	theme	blood	372:376	arg1	options					394:400	different blood anticoagulation options	362:400	different blood anticoagulation options	362:400	OBJECTIVE Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.
35253976	6	70	theme	platelet	1201:1208	arg1	samples					1210:1216	all prepared platelet samples	1188:1216	all prepared platelet samples	1188:1216	Following platelet isolation, TMT-MS quantified 3357 proteins amongst all prepared platelet samples.
35253976	3	71	theme	tag	769:771	arg1	labeling					779:786	multiplexed tandem mass tag (TMT) labeling	745:786	multiplexed tandem mass tag (TMT) labeling	745:786	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	10	72	theme	methodology-associated	1969:1990	arg1	alterations					1992:2002	methodology-associated alterations	1969:2002	methodology-associated alterations in platelet proteome	1969:2023	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	3	73	theme	triple	792:797	arg1	spectrometry					815:826	triple quadrupole mass spectrometry	792:826	triple quadrupole mass spectrometry (MS)	792:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	73	theme	triple	792:797	arg1	MS					829:830	MS	829:830	MS	829:830	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	9	74	theme	higher	1636:1641	arg1	levels					1643:1648	higher levels	1636:1648	higher levels of histone and neutrophil-associated proteins	1636:1694	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	5	75	theme	platelet	1088:1095	arg1	secretion					1107:1115	platelet α-granule secretion	1088:1115	platelet α-granule secretion	1088:1115	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	1	76	theme	Ex	124:125	arg1	assays					132:137	Ex vivo assays	124:137	Ex vivo assays of platelet function	124:158	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	0	77	theme	time	70:73	arg1	Effects					0:6	Effects	0:6	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.	0:111	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	4	78	dep	RESULTS	834:840	arg1	had					861:863	had	861:863	had time-dependent effects on platelet function in whole blood	861:922	RESULTS Each anticoagulant had time-dependent effects on platelet function in whole blood.
35253976	3	79	theme	mass	810:813	arg1	spectrometry					815:826	triple quadrupole mass spectrometry	792:826	triple quadrupole mass spectrometry (MS)	792:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	79	theme	mass	810:813	arg1	MS					829:830	MS	829:830	MS	829:830	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	1	80	theme	platelet	142:149	arg1	function					151:158	platelet function	142:158	platelet function	142:158	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	0	81	dep	ex	11:12	arg1	vivo					14:17	vivo	14:17	vivo	14:17	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	0	82	theme	platelets	102:110	arg1	content					91:97	the proteome content	78:97	the proteome content of platelets	78:110	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	9	83	theme	heparinized	1614:1624	arg1	blood					1626:1630	heparinized blood	1614:1630	heparinized blood	1614:1630	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	5	84	theme	aggregate	1003:1011	arg1	formation					1013:1021	platelet-monocyte aggregate formation	985:1021	platelet-monocyte aggregate formation	985:1021	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	9	85	from	interactions	1782:1793	arg1	blood					1804:1808	whole blood	1798:1808	whole blood	1798:1808	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	8	86	theme	platelet	1456:1463	arg1	proteomes					1465:1473	platelet proteomes	1456:1473	platelet proteomes	1456:1473	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	2	87	dep	OBJECTIVE	330:338	arg1	evaluate					349:356	evaluate	349:356	evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo	349:474	OBJECTIVE Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.
35253976	2	88	theme	anticoagulation	378:392	arg1	options					394:400	different blood anticoagulation options	362:400	different blood anticoagulation options	362:400	OBJECTIVE Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.
35253976	3	89	dep	METHODS	477:483	arg1	processed					614:622	processed	614:622	processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS)	614:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	89	dep	METHODS	477:483	arg1	collected					513:521	collected	513:521	collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin	513:607	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	5	90	theme	agonist	965:971	arg1	reactivity					973:982	platelet agonist reactivity	956:982	platelet agonist reactivity	956:982	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	10	91	from	alterations	1992:2002	arg1	proteome					2016:2023	platelet proteome	2007:2023	platelet proteome	2007:2023	CONCLUSION Our results demonstrate that different anticoagulants routinely used for blood collection have varying effects on platelets ex vivo, where methodology-associated alterations in platelet proteome may influence mechanistic, translational and biomarker studies.
35253976	7	92	theme	membrane	1385:1392	arg1	trafficking					1394:1404	membrane trafficking	1385:1404	membrane trafficking	1385:1404	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	9	93	theme	related	1708:1714	arg1	manner					1701:1706	a manner	1699:1706	a manner related to neutrophil extracellular trap (NET) formation and platelet	1699:1776	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	8	94	theme	complement	1494:1503	arg1	system					1505:1510	increased complement system	1484:1510	increased complement system	1484:1510	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	2	95	theme	platelet	430:437	arg1	function					439:446	platelet function	430:446	platelet function	430:446	OBJECTIVE Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.
35253976	0	96	theme	anticoagulation	25:39	arg1	Effects					0:6	Effects	0:6	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.	0:111	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	9	97	theme	NET	1750:1752	arg1	formation					1755:1763	extracellular trap (NET) formation	1730:1763	extracellular trap (NET) formation	1730:1763	Platelets prepared from heparinized blood had higher levels of histone and neutrophil-associated proteins in a manner related to neutrophil extracellular trap (NET) formation and platelet:NET interactions in whole blood ex vivo.
35253976	7	98	theme	pertinent	1372:1380	arg1	proteins					1363:1370	proteins	1363:1370	proteins pertinent to membrane trafficking and exocytosis	1363:1419	Altogether, >400 proteins were differentially abundant in platelets isolated from blood processed at 24 h versus 1 h post-phlebotomy, including proteins pertinent to membrane trafficking and exocytosis.
35253976	3	99	theme	extended	632:639	arg1	course					646:651	an extended time course	629:651	an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS)	629:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	0	100	theme	preanalytical	45:57	arg1	time					70:73	preanalytical processing time	45:73	preanalytical processing time	45:73	Effects of ex vivo blood anticoagulation and preanalytical processing time on the proteome content of platelets.
35253976	6	101	theme	prepared	1192:1199	arg1	samples					1210:1216	all prepared platelet samples	1188:1216	all prepared platelet samples	1188:1216	Following platelet isolation, TMT-MS quantified 3357 proteins amongst all prepared platelet samples.
35253976	3	102	theme	biochemical	672:682	arg1	quantification					725:738	platelet proteome quantification	707:738	platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS)	707:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	102	theme	biochemical	672:682	arg1	experiments					684:694	functional and biochemical experiments	657:694	functional and biochemical experiments	657:694	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	8	103	theme	α-granule	1526:1534	arg1	proteins					1536:1543	α-granule proteins	1526:1543	α-granule proteins in platelets from EDTA-anticoagulated blood	1526:1587	Anticoagulant-specific effects on platelet proteomes included increased complement system and decreased α-granule proteins in platelets from EDTA-anticoagulated blood.
35253976	3	104	with	quantification	725:738	arg1	spectrometry					815:826	triple quadrupole mass spectrometry	792:826	triple quadrupole mass spectrometry (MS)	792:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	104	with	quantification	725:738	arg1	labeling					779:786	multiplexed tandem mass tag (TMT) labeling	745:786	multiplexed tandem mass tag (TMT) labeling	745:786	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	104	with	quantification	725:738	arg1	MS					829:830	MS	829:830	MS	829:830	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	1	105	dep	BACKGROUND	113:122	arg1	inform					171:176	inform	171:176	inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified	171:327	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
35253976	3	106	theme	functional	657:666	arg1	quantification					725:738	platelet proteome quantification	707:738	platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS)	707:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	3	106	theme	functional	657:666	arg1	experiments					684:694	functional and biochemical experiments	657:694	functional and biochemical experiments	657:694	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	5	107	theme	extracellular	1036:1048	arg1	release					1058:1064	platelet extracellular vesicle release	1027:1064	platelet extracellular vesicle release	1027:1064	For instance, heparin enhanced platelet agonist reactivity, platelet-monocyte aggregate formation and platelet extracellular vesicle release, while EDTA increased platelet α-granule secretion.
35253976	6	108	theme	3357	1166:1169	arg1	proteins					1171:1178	3357 proteins	1166:1178	3357 proteins	1166:1178	Following platelet isolation, TMT-MS quantified 3357 proteins amongst all prepared platelet samples.
35253976	3	109	theme	platelet	707:714	arg1	quantification					725:738	platelet proteome quantification	707:738	platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS)	707:831	METHODS Parallel blood samples were collected from healthy human donors into sodium citrate, acid citrate dextrose, EDTA or heparin, and processed over an extended time course for functional and biochemical experiments, including platelet proteome quantification with multiplexed tandem mass tag (TMT) labeling and triple quadrupole mass spectrometry (MS).
35253976	2	110	theme	different	362:370	arg1	options					394:400	different blood anticoagulation options	362:400	different blood anticoagulation options	362:400	OBJECTIVE Here, we evaluate how different blood anticoagulation options and processing times affect platelet function and protein content ex vivo.
35253976	1	111	theme	divergent	240:248	arg1	methods					267:273	divergent blood processing methods	240:273	divergent blood processing methods	240:273	BACKGROUND Ex vivo assays of platelet function critically inform mechanistic and clinical hematology studies, where effects of divergent blood processing methods on platelet composition are apparent, but unspecified.
36213313	6	0	theme	protein	974:980	arg1	repeats					1005:1011	the disaccharide repeats	988:1011	the disaccharide repeats	988:1011	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	6	0	theme	protein	974:980	arg1	part					982:985	the protein part	970:985	the protein part	970:985	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	3	1	attach	attached	517:524	arg2	chains					510:515	the GAG chains	502:515	the GAG chains attached to the core protein	502:544	These pleiotropic functions of proteoglycans are regulated spatiotemporally by the GAG chains attached to the core protein.
36213313	3	1	attach	attached	517:524	arg1	protein					538:544	the core protein	529:544	the core protein	529:544	These pleiotropic functions of proteoglycans are regulated spatiotemporally by the GAG chains attached to the core protein.
36213313	4	2	theme	cerebrospinal	625:637	arg1	fluid					639:643	cerebrospinal fluid	625:643	cerebrospinal fluid	625:643	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	4	2	theme	cerebrospinal	625:637	arg1	proteoglycans					592:604	70 chondroitin sulfate-linked proteoglycans	562:604	70 chondroitin sulfate-linked proteoglycans reported in cells	562:622	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	11	3	theme	composition	2026:2036	arg1	search					2038:2043	hybrid database search/de novo glycan composition search	1988:2043	hybrid database search/de novo glycan composition search	1988:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	10	4	theme	intact	1648:1653	arg1	peptides					1666:1673	intact GAG-linked peptides	1648:1673	intact GAG-linked peptides	1648:1673	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	11	5	theme	intact	1760:1765	arg1	glycopeptides					1767:1779	These intact glycopeptides	1754:1779	These intact glycopeptides including their core linker glycans	1754:1815	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	7	6	theme	oligosaccharides	1148:1163	arg1	composition					1126:1136	the composition	1122:1136	the composition of linker oligosaccharides	1122:1163	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	6	theme	oligosaccharides	1148:1163	arg1	degree					1173:1178	the degree	1169:1178	the degree of sulfation and/or phosphorylation	1169:1214	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	6	theme	oligosaccharides	1148:1163	arg1	attachment					1108:1117	attachment	1108:1117	attachment	1108:1117	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	6	7	theme	current	855:861	arg1	paradigm					863:870	The current paradigm	851:870	The current paradigm of CSPG analysis	851:887	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	11	8	theme	novo	2014:2017	arg1	search					2038:2043	hybrid database search/de novo glycan composition search	1988:2043	hybrid database search/de novo glycan composition search	1988:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	2	9	theme	signaling	403:411	arg1	proteins					413:420	signaling proteins	403:420	signaling proteins	403:420	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	8	10	theme	novel	1293:1297	arg1	CSPGs					1299:1303	novel CSPGs	1293:1303	novel CSPGs	1293:1303	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	14	11	theme	available	2420:2428	arg1	material					2411:2418	supplementary material	2397:2418	supplementary material available at 10.1007/s42485-022-00092-3	2397:2458	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	1	12	theme	side	189:192	arg1	chains					194:199	linear glycosaminoglycan (GAG) side chains	158:199	linear glycosaminoglycan (GAG) side chains attached to a core protein	158:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	6	13	theme	chondroitinase	926:939	arg1	enzymes					941:947	chondroitinase enzymes	926:947	chondroitinase enzymes	926:947	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	14	14	theme	supplementary	2397:2409	arg1	material					2411:2418	supplementary material	2397:2418	supplementary material available at 10.1007/s42485-022-00092-3	2397:2458	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	11	15	theme	electron-transfer/higher	1919:1942	arg1	dissociation					1961:1972	electron-transfer/higher energy collision dissociation	1919:1972	electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search	1919:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	13	16	theme	fragmentation	2286:2298	arg1	methods					2300:2306	alternative fragmentation methods	2274:2306	alternative fragmentation methods for the characterization of CSPGs	2274:2340	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	8	17	theme	mass	1242:1245	arg1	spectrometry					1247:1258	mass spectrometry	1242:1258	mass spectrometry	1242:1258	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	7	18	theme	attachment	1108:1117	arg1	composition					1126:1136	the composition	1122:1136	the composition of linker oligosaccharides	1122:1163	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	18	theme	attachment	1108:1117	arg1	site					1100:1103	the site	1096:1103	the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation	1096:1214	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	18	theme	attachment	1108:1117	arg1	attachment					1108:1117	attachment	1108:1117	attachment	1108:1117	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	2	19	theme	cellular	295:302	arg1	proliferation					304:316	cellular proliferation	295:316	cellular proliferation	295:316	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	2	19	theme	cellular	295:302	arg1	neurodevelopment					256:271	neurodevelopment	256:271	neurodevelopment	256:271	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	9	20	theme	searching	1530:1538	arg1	approaches					1540:1549	database searching approaches	1521:1549	database searching approaches	1521:1549	Unknown identity of the linker oligosaccharide also makes it more difficult to identify the glycan composition using database searching approaches.
36213313	5	21	theme	disaccharide	763:774	arg1	units					786:790	20-200 disaccharide repeating units	756:790	20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze	756:848	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	13	22	theme	CSPGs	2336:2340	arg1	characterization					2316:2331	the characterization	2312:2331	the characterization of CSPGs	2312:2340	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	6	23	theme	disaccharide	992:1003	arg1	repeats					1005:1011	the disaccharide repeats	988:1011	the disaccharide repeats	988:1011	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	6	23	theme	disaccharide	992:1003	arg1	part					982:985	the protein part	970:985	the protein part	970:985	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	14	24	theme	Supplementary	2343:2355	arg1	Information					2357:2367	Supplementary Information	2343:2367	Supplementary Information The online version	2343:2386	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	11	25	theme	collision	1951:1959	arg1	dissociation					1961:1972	electron-transfer/higher energy collision dissociation	1919:1972	electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search	1919:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	11	26	dep	stepped	1870:1876	arg1	higher					1878:1883	higher	1878:1883	higher	1878:1883	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	8	27	from	challenges	1330:1339	arg1	isolation					1350:1358	their isolation	1344:1358	their isolation	1344:1358	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	8	27	from	challenges	1330:1339	arg1	ionization					1374:1383	less optimal ionization	1361:1383	less optimal ionization	1361:1383	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	8	27	from	challenges	1330:1339	arg1	analysis					1394:1401	data analysis	1389:1401	data analysis	1389:1401	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	8	28	theme	optimal	1366:1372	arg1	ionization					1374:1383	less optimal ionization	1361:1383	less optimal ionization	1361:1383	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	11	29	theme	mass	1836:1839	arg1	spectrometry					1841:1852	mass spectrometry	1836:1852	mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search	1836:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	13	30	theme	spectrometry	2242:2253	arg1	analysis					2255:2262	optimized high-resolution mass spectrometry analysis	2211:2262	optimized high-resolution mass spectrometry analysis	2211:2262	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	2	31	theme	vital	242:246	arg1	role					248:251	a vital role	240:251	a vital role	240:251	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	1	32	theme	glycosaminoglycan	165:181	arg1	chains					194:199	linear glycosaminoglycan (GAG) side chains	158:199	linear glycosaminoglycan (GAG) side chains attached to a core protein	158:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	11	33	theme	energy	1885:1890	arg1	dissociation					1902:1913	optimized stepped higher energy collision dissociation	1860:1913	optimized stepped higher energy collision dissociation	1860:1913	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	5	34	theme	specific	712:719	arg1	residues					728:735	specific serine residues	712:735	specific serine residues	712:735	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	11	35	theme	stepped	1870:1876	arg1	dissociation					1902:1913	optimized stepped higher energy collision dissociation	1860:1913	optimized stepped higher energy collision dissociation	1860:1913	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	12	36	theme	novel	2085:2089	arg1	CSPGs					2091:2095	three novel CSPGs	2079:2095	three novel CSPGs that have not been described earlier	2079:2132	We identified 25 CSPGs including three novel CSPGs that have not been described earlier.
36213313	1	37	theme	sulfate	80:86	arg1	proteoglycans					88:100	Chondroitin sulfate proteoglycans	68:100	Chondroitin sulfate proteoglycans (CSPGs)	68:108	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	37	theme	sulfate	80:86	arg1	components					135:144	extracellular matrix components	114:144	extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein	114:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	37	theme	sulfate	80:86	arg1	CSPGs					103:107	CSPGs	103:107	CSPGs	103:107	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	6	38	theme	mass	1025:1028	arg1	spectrometry					1030:1041	mass spectrometry	1025:1041	mass spectrometry	1025:1041	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	5	39	theme	intact	799:804	arg1	CSPGs					806:810	intact CSPGs	799:810	intact CSPGs very large and impractical to analyze	799:848	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	13	40	theme	high-resolution	2221:2235	arg1	analysis					2255:2262	optimized high-resolution mass spectrometry analysis	2211:2262	optimized high-resolution mass spectrometry analysis	2211:2262	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	5	41	theme	impractical	827:837	arg1	CSPGs					806:810	intact CSPGs	799:810	intact CSPGs very large and impractical to analyze	799:848	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	11	42	theme	optimized	1860:1868	arg1	dissociation					1902:1913	optimized stepped higher energy collision dissociation	1860:1913	optimized stepped higher energy collision dissociation	1860:1913	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	14	43	dep	Information	2357:2367	arg1	version					2380:2386	The online version	2369:2386	Supplementary Information The online version	2343:2386	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	3	44	theme	proteoglycans	454:466	arg1	functions					441:449	These pleiotropic functions	423:449	These pleiotropic functions of proteoglycans	423:466	These pleiotropic functions of proteoglycans are regulated spatiotemporally by the GAG chains attached to the core protein.
36213313	0	45	theme	Mass	0:3	arg1	analysis					19:26	Mass spectrometric analysis	0:26	Mass spectrometric analysis of chondroitin	0:41	Mass spectrometric analysis of chondroitin sulfate-linked peptides.
36213313	3	46	theme	GAG	506:508	arg1	chains					510:515	the GAG chains	502:515	the GAG chains attached to the core protein	502:544	These pleiotropic functions of proteoglycans are regulated spatiotemporally by the GAG chains attached to the core protein.
36213313	1	47	theme	extracellular	114:126	arg1	proteoglycans					88:100	Chondroitin sulfate proteoglycans	68:100	Chondroitin sulfate proteoglycans (CSPGs)	68:108	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	47	theme	extracellular	114:126	arg1	components					135:144	extracellular matrix components	114:144	extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein	114:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	10	48	theme	chondroitinase	1562:1575	arg1	digestion					1577:1585	chondroitinase digestion	1562:1585	chondroitinase digestion of long GAG chains linked to tryptic peptides	1562:1631	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	11	49	theme	linker	1802:1807	arg1	glycans					1809:1815	their core linker glycans	1791:1815	their core linker glycans	1791:1815	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	11	50	gly	glycopeptides	1767:1779	arg2	glycopeptides					1767:1779	These intact glycopeptides	1754:1779	These intact glycopeptides including their core linker glycans	1754:1815	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	0	51	theme	chondroitin	31:41	arg1	analysis					19:26	Mass spectrometric analysis	0:26	Mass spectrometric analysis of chondroitin	0:41	Mass spectrometric analysis of chondroitin sulfate-linked peptides.
36213313	6	52	theme	GAG	912:914	arg1	chains					916:921	the GAG chains	908:921	the GAG chains	908:921	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	5	53	dep	glycan	663:668	arg1	linker					670:675	linker	670:675	A core glycan linker of 3-6 monosaccharides attached to specific serine residues	656:735	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	11	54	theme	search/de	2004:2012	arg1	search					2038:2043	hybrid database search/de novo glycan composition search	1988:2043	hybrid database search/de novo glycan composition search	1988:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	4	55	theme	chondroitin	565:575	arg1	fluid					639:643	cerebrospinal fluid	625:643	cerebrospinal fluid	625:643	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	4	55	theme	chondroitin	565:575	arg1	urine					649:653	urine	649:653	urine	649:653	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	4	55	theme	chondroitin	565:575	arg1	proteoglycans					592:604	70 chondroitin sulfate-linked proteoglycans	562:604	70 chondroitin sulfate-linked proteoglycans reported in cells	562:622	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	10	56	theme	GAG	1595:1597	arg1	chains					1599:1604	long GAG chains	1590:1604	long GAG chains linked to tryptic peptides	1590:1631	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	2	57	theme	tumor	342:346	arg1	metastasis					348:357	tumor metastasis	342:357	tumor metastasis	342:357	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	2	57	theme	tumor	342:346	arg1	neurodevelopment					256:271	neurodevelopment	256:271	neurodevelopment	256:271	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	9	58	theme	oligosaccharide	1435:1449	arg1	identity					1412:1419	Unknown identity	1404:1419	Unknown identity of the linker oligosaccharide	1404:1449	Unknown identity of the linker oligosaccharide also makes it more difficult to identify the glycan composition using database searching approaches.
36213313	6	59	theme	analysis	880:887	arg1	paradigm					863:870	The current paradigm	851:870	The current paradigm of CSPG analysis	851:887	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	9	60	theme	glycan	1496:1501	arg1	composition					1503:1513	the glycan composition	1492:1513	the glycan composition using database searching approaches	1492:1549	Unknown identity of the linker oligosaccharide also makes it more difficult to identify the glycan composition using database searching approaches.
36213313	10	61	theme	tryptic	1616:1622	arg1	peptides					1624:1631	tryptic peptides	1616:1631	tryptic peptides	1616:1631	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	5	62	theme	monosaccharides	684:698	arg1	glycan					663:668	A core glycan	656:668	A core glycan linker of 3-6 monosaccharides attached to specific serine residues	656:735	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	11	63	theme	glycan	2019:2024	arg1	search					2038:2043	hybrid database search/de novo glycan composition search	1988:2043	hybrid database search/de novo glycan composition search	1988:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	10	64	theme	GAG-linked	1655:1664	arg1	peptides					1666:1673	intact GAG-linked peptides	1648:1673	intact GAG-linked peptides	1648:1673	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	7	65	theme	linker	1141:1146	arg1	oligosaccharides					1148:1163	linker oligosaccharides	1141:1163	linker oligosaccharides	1141:1163	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	4	66	link	sulfate-linked	577:590	arg1	fluid					639:643	cerebrospinal fluid	625:643	cerebrospinal fluid	625:643	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	4	66	link	sulfate-linked	577:590	arg1	urine					649:653	urine	649:653	urine	649:653	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	4	66	link	sulfate-linked	577:590	arg1	proteoglycans					592:604	70 chondroitin sulfate-linked proteoglycans	562:604	70 chondroitin sulfate-linked proteoglycans reported in cells	562:622	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	7	67	theme	composition	1126:1136	arg1	composition					1126:1136	the composition	1122:1136	the composition of linker oligosaccharides	1122:1163	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	67	theme	composition	1126:1136	arg1	site					1100:1103	the site	1096:1103	the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation	1096:1214	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	67	theme	composition	1126:1136	arg1	attachment					1108:1117	attachment	1108:1117	attachment	1108:1117	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	2	68	theme	signal	274:279	arg1	transduction					281:292	signal transduction	274:292	signal transduction	274:292	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	2	68	theme	signal	274:279	arg1	neurodevelopment					256:271	neurodevelopment	256:271	neurodevelopment	256:271	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	10	69	theme	relevant	1689:1696	arg1	urine					1724:1728	urine	1724:1728	urine	1724:1728	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	10	69	theme	relevant	1689:1696	arg1	fibroblasts					1741:1751	dermal fibroblasts	1734:1751	dermal fibroblasts	1734:1751	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	10	69	theme	relevant	1689:1696	arg1	plasma					1716:1721	plasma	1716:1721	plasma	1716:1721	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	10	69	theme	relevant	1689:1696	arg1	samples					1698:1704	clinically relevant samples	1678:1704	clinically relevant samples including plasma, urine and dermal fibroblasts	1678:1751	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	8	70	theme	CSPGs	1299:1303	arg1	analysis					1230:1237	the analysis	1226:1237	the analysis by mass spectrometry	1226:1258	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	8	70	theme	CSPGs	1299:1303	arg1	identification					1275:1288	subsequent identification	1264:1288	subsequent identification of novel CSPGs	1264:1303	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	11	71	theme	energy	1944:1949	arg1	dissociation					1961:1972	electron-transfer/higher energy collision dissociation	1919:1972	electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search	1919:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	9	72	theme	database	1521:1528	arg1	searching					1530:1538	database searching	1521:1538	database searching approaches	1521:1549	Unknown identity of the linker oligosaccharide also makes it more difficult to identify the glycan composition using database searching approaches.
36213313	14	73	theme	online	2373:2378	arg1	version					2380:2386	The online version	2369:2386	Supplementary Information The online version	2343:2386	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	3	74	theme	core	533:536	arg1	protein					538:544	the core protein	529:544	the core protein	529:544	These pleiotropic functions of proteoglycans are regulated spatiotemporally by the GAG chains attached to the core protein.
36213313	5	75	theme	20-200	756:761	arg1	units					786:790	20-200 disaccharide repeating units	756:790	20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze	756:848	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	1	76	theme	core	215:218	arg1	protein					220:226	a core protein	213:226	a core protein	213:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	8	77	theme	technical	1320:1328	arg1	challenges					1330:1339	technical challenges	1320:1339	technical challenges in their isolation, less optimal ionization and data analysis	1320:1401	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	5	78	theme	serine	721:726	arg1	residues					728:735	specific serine residues	712:735	specific serine residues	712:735	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	1	79	theme	matrix	128:133	arg1	proteoglycans					88:100	Chondroitin sulfate proteoglycans	68:100	Chondroitin sulfate proteoglycans (CSPGs)	68:108	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	79	theme	matrix	128:133	arg1	components					135:144	extracellular matrix components	114:144	extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein	114:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	8	80	theme	subsequent	1264:1273	arg1	identification					1275:1288	subsequent identification	1264:1288	subsequent identification of novel CSPGs	1264:1303	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	5	81	attach	attached	700:707	arg2	glycan					663:668	A core glycan	656:668	A core glycan linker of 3-6 monosaccharides attached to specific serine residues	656:735	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	5	81	attach	attached	700:707	arg1	residues					728:735	specific serine residues	712:735	specific serine residues	712:735	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	11	82	theme	hybrid	1988:1993	arg1	search					2038:2043	hybrid database search/de novo glycan composition search	1988:2043	hybrid database search/de novo glycan composition search	1988:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	1	83	theme	linear	158:163	arg1	glycosaminoglycan					165:181	linear glycosaminoglycan	158:181	linear glycosaminoglycan (GAG) side chains attached to a core protein	158:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	83	theme	linear	158:163	arg1	GAG					184:186	GAG	184:186	GAG	184:186	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	5	84	theme	large	817:821	arg1	CSPGs					806:810	intact CSPGs	799:810	intact CSPGs very large and impractical to analyze	799:848	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	11	85	theme	collision	1892:1900	arg1	dissociation					1902:1913	optimized stepped higher energy collision dissociation	1860:1913	optimized stepped higher energy collision dissociation	1860:1913	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	5	86	theme	repeating	776:784	arg1	units					786:790	20-200 disaccharide repeating units	756:790	20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze	756:848	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	13	87	theme	alternative	2274:2284	arg1	methods					2300:2306	alternative fragmentation methods	2274:2306	alternative fragmentation methods for the characterization of CSPGs	2274:2340	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	1	88	theme	Chondroitin	68:78	arg1	proteoglycans					88:100	Chondroitin sulfate proteoglycans	68:100	Chondroitin sulfate proteoglycans (CSPGs)	68:108	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	88	theme	Chondroitin	68:78	arg1	components					135:144	extracellular matrix components	114:144	extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein	114:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	88	theme	Chondroitin	68:78	arg1	CSPGs					103:107	CSPGs	103:107	CSPGs	103:107	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	8	89	theme	data	1389:1392	arg1	analysis					1394:1401	data analysis	1389:1401	data analysis	1389:1401	Further, the analysis by mass spectrometry and subsequent identification of novel CSPGs is hampered by technical challenges in their isolation, less optimal ionization and data analysis.
36213313	13	90	theme	optimized	2211:2219	arg1	analysis					2255:2262	optimized high-resolution mass spectrometry analysis	2211:2262	optimized high-resolution mass spectrometry analysis	2211:2262	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	5	91	theme	core	658:661	arg1	glycan					663:668	A core glycan	656:668	A core glycan linker of 3-6 monosaccharides attached to specific serine residues	656:735	A core glycan linker of 3-6 monosaccharides attached to specific serine residues can be extended by 20-200 disaccharide repeating units making intact CSPGs very large and impractical to analyze.
36213313	14	92	from	10.1007/s42485-022-00092-3	2433:2458	arg1	available					2420:2428	available	2420:2428	available	2420:2428	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	10	93	theme	dermal	1734:1739	arg1	fibroblasts					1741:1751	dermal fibroblasts	1734:1751	dermal fibroblasts	1734:1751	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	0	94	theme	spectrometric	5:17	arg1	analysis					19:26	Mass spectrometric analysis	0:26	Mass spectrometric analysis of chondroitin	0:41	Mass spectrometric analysis of chondroitin sulfate-linked peptides.
36213313	13	95	theme	mass	2237:2240	arg1	analysis					2255:2262	optimized high-resolution mass spectrometry analysis	2211:2262	optimized high-resolution mass spectrometry analysis	2211:2262	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	7	96	theme	phosphorylation	1200:1214	arg1	composition					1126:1136	the composition	1122:1136	the composition of linker oligosaccharides	1122:1163	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	96	theme	phosphorylation	1200:1214	arg1	degree					1173:1178	the degree	1169:1178	the degree of sulfation and/or phosphorylation	1169:1214	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	96	theme	phosphorylation	1200:1214	arg1	attachment					1108:1117	attachment	1108:1117	attachment	1108:1117	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	11	97	theme	core	1797:1800	arg1	glycans					1809:1815	their core linker glycans	1791:1815	their core linker glycans	1791:1815	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	7	98	theme	sulfation	1183:1191	arg1	composition					1126:1136	the composition	1122:1136	the composition of linker oligosaccharides	1122:1163	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	98	theme	sulfation	1183:1191	arg1	degree					1173:1178	the degree	1169:1178	the degree of sulfation and/or phosphorylation	1169:1214	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	7	98	theme	sulfation	1183:1191	arg1	attachment					1108:1117	attachment	1108:1117	attachment	1108:1117	This method, however, provides no information about the site of attachment or the composition of linker oligosaccharides and the degree of sulfation and/or phosphorylation.
36213313	9	99	theme	Unknown	1404:1410	arg1	identity					1412:1419	Unknown identity	1404:1419	Unknown identity of the linker oligosaccharide	1404:1449	Unknown identity of the linker oligosaccharide also makes it more difficult to identify the glycan composition using database searching approaches.
36213313	1	100	attach	attached	201:208	arg1	protein					220:226	a core protein	213:226	a core protein	213:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	1	100	attach	attached	201:208	arg2	chains					194:199	linear glycosaminoglycan (GAG) side chains	158:199	linear glycosaminoglycan (GAG) side chains attached to a core protein	158:226	Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein.
36213313	3	101	theme	pleiotropic	429:439	arg1	functions					441:449	These pleiotropic functions	423:449	These pleiotropic functions of proteoglycans	423:466	These pleiotropic functions of proteoglycans are regulated spatiotemporally by the GAG chains attached to the core protein.
36213313	4	102	theme	sulfate-linked	577:590	arg1	fluid					639:643	cerebrospinal fluid	625:643	cerebrospinal fluid	625:643	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	4	102	theme	sulfate-linked	577:590	arg1	urine					649:653	urine	649:653	urine	649:653	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	4	102	theme	sulfate-linked	577:590	arg1	proteoglycans					592:604	70 chondroitin sulfate-linked proteoglycans	562:604	70 chondroitin sulfate-linked proteoglycans reported in cells	562:622	There are over 70 chondroitin sulfate-linked proteoglycans reported in cells, cerebrospinal fluid and urine.
36213313	10	103	theme	chains	1599:1604	arg1	digestion					1577:1585	chondroitinase digestion	1562:1585	chondroitinase digestion of long GAG chains linked to tryptic peptides	1562:1631	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	13	104	theme	enrichment	2185:2194	arg1	methods					2300:2306	alternative fragmentation methods	2274:2306	alternative fragmentation methods for the characterization of CSPGs	2274:2340	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	13	104	theme	enrichment	2185:2194	arg1	strategies					2196:2205	enrichment strategies	2185:2205	enrichment strategies	2185:2205	Our findings demonstrate the utility of combining enrichment strategies and optimized high-resolution mass spectrometry analysis including alternative fragmentation methods for the characterization of CSPGs.
36213313	10	105	attach	linked	1606:1611	arg1	peptides					1624:1631	tryptic peptides	1616:1631	tryptic peptides	1616:1631	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	10	105	attach	linked	1606:1611	arg2	chains					1599:1604	long GAG chains	1590:1604	long GAG chains linked to tryptic peptides	1590:1631	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	9	106	theme	linker	1428:1433	arg1	oligosaccharide					1435:1449	the linker oligosaccharide	1424:1449	the linker oligosaccharide	1424:1449	Unknown identity of the linker oligosaccharide also makes it more difficult to identify the glycan composition using database searching approaches.
36213313	2	107	with	interaction	367:377	arg1	proteins					413:420	signaling proteins	403:420	signaling proteins	403:420	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	2	107	with	interaction	367:377	arg1	factors					391:397	growth factors	384:397	growth factors	384:397	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
36213313	14	108	contain	contains	2388:2395	arg2	material					2411:2418	supplementary material	2397:2418	supplementary material available at 10.1007/s42485-022-00092-3	2397:2458	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	14	108	contain	contains	2388:2395	arg1	Information					2357:2367	Supplementary Information	2343:2367	Supplementary Information The online version	2343:2386	Supplementary Information The online version contains supplementary material available at 10.1007/s42485-022-00092-3.
36213313	10	109	theme	long	1590:1593	arg1	chains					1599:1604	long GAG chains	1590:1604	long GAG chains linked to tryptic peptides	1590:1631	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	11	110	theme	database	1995:2002	arg1	search					2038:2043	hybrid database search/de novo glycan composition search	1988:2043	hybrid database search/de novo glycan composition search	1988:2043	These intact glycopeptides including their core linker glycans were identified by mass spectrometry using optimized stepped higher energy collision dissociation and electron-transfer/higher energy collision dissociation combined with hybrid database search/de novo glycan composition search.
36213313	6	111	theme	CSPG	875:878	arg1	analysis					880:887	CSPG analysis	875:887	CSPG analysis	875:887	The current paradigm of CSPG analysis involves digesting the GAG chains by chondroitinase enzymes and analyzing either the protein part, the disaccharide repeats, or both by mass spectrometry.
36213313	10	112	link	GAG-linked	1655:1664	arg1	peptides					1666:1673	intact GAG-linked peptides	1648:1673	intact GAG-linked peptides	1648:1673	Following chondroitinase digestion of long GAG chains linked to tryptic peptides, we identified intact GAG-linked peptides in clinically relevant samples including plasma, urine and dermal fibroblasts.
36213313	2	113	theme	growth	384:389	arg1	factors					391:397	growth factors	384:397	growth factors	384:397	CSPGs play a vital role in neurodevelopment, signal transduction, cellular proliferation and differentiation and tumor metastasis through interaction with growth factors and signaling proteins.
37299358	0	0	from	Effect	15:20	arg1	Preparation					42:52	the Preparation	38:52	the Preparation of a Conductive Porous Composite	38:85	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	2	1	from	waste	361:365	arg1	starch					342:347	starch	342:347	starch from potato waste	342:365	Biopolymers are obtained from natural sources, for instance, starch from potato waste, and its conversion into value-added products is highly significant in a circular economy.
37299358	0	2	theme	Conductive	59:68	arg1	Composite					77:85	a Conductive Porous Composite	57:85	a Conductive Porous Composite	57:85	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	5	3	theme	soaking	930:936	arg1	time					938:941	a longer soaking time	921:941	a longer soaking time	921:941	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	6	4	theme	electronic	1175:1184	arg1	fields					1217:1222	electronic, environmental, and biological fields	1175:1222	electronic, environmental, and biological fields	1175:1222	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	3	5	theme	starch-based	470:481	arg1	polymerized					506:516	polymerized	506:516	polymerized	506:516	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	3	5	theme	starch-based	470:481	arg1	cryogel					494:500	The biomass starch-based conductive cryogel	458:500	The biomass starch-based conductive cryogel	458:500	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	4	6	theme	chemical	707:714	arg1	properties					716:725	Thermal, spectrophotometric, physical, and chemical properties	664:725	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III),	664:767	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	3	7	theme	conductive	483:492	arg1	polymerized					506:516	polymerized	506:516	polymerized	506:516	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	3	7	theme	conductive	483:492	arg1	cryogel					494:500	The biomass starch-based conductive cryogel	458:500	The biomass starch-based conductive cryogel	458:500	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	1	8	theme	conductive	194:203	arg1	composite					212:220	a conductive porous composite	192:220	a conductive porous composite using a biomass waste-based starch template	192:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	2	9	theme	circular	440:447	arg1	economy					449:455	a circular economy	438:455	a circular economy	438:455	Biopolymers are obtained from natural sources, for instance, starch from potato waste, and its conversion into value-added products is highly significant in a circular economy.
37299358	0	10	theme	Porous	70:75	arg1	Composite					77:85	a Conductive Porous Composite	57:85	a Conductive Porous Composite	57:85	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	6	11	theme	biological	1206:1215	arg1	fields					1217:1222	electronic, environmental, and biological fields	1175:1222	electronic, environmental, and biological fields	1175:1222	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	3	12	theme	biomass	462:468	arg1	polymerized					506:516	polymerized	506:516	polymerized	506:516	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	3	12	theme	biomass	462:468	arg1	cryogel					494:500	The biomass starch-based conductive cryogel	458:500	The biomass starch-based conductive cryogel	458:500	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	0	13	theme	Composite	77:85	arg1	Preparation					42:52	the Preparation	38:52	the Preparation of a Conductive Porous Composite	38:85	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	4	14	theme	polymer	788:794	arg1	template					741:748	the starch template	730:748	the starch template	730:748	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	4	14	theme	polymer	788:794	arg1	composites					796:805	the conductive polymer composites	773:805	the conductive polymer composites	773:805	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	4	15	theme	physical	693:700	arg1	properties					716:725	Thermal, spectrophotometric, physical, and chemical properties	664:725	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III),	664:767	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	1	16	theme	porous	205:210	arg1	composite					212:220	a conductive porous composite	192:220	a conductive porous composite using a biomass waste-based starch template	192:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	4	17	theme	conductive	777:786	arg1	template					741:748	the starch template	730:748	the starch template	730:748	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	4	17	theme	conductive	777:786	arg1	composites					796:805	the conductive polymer composites	773:805	the conductive polymer composites	773:805	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	1	18	theme	composite	212:220	arg1	preparation					177:187	the preparation	173:187	the preparation of a conductive porous composite using a biomass waste-based starch template	173:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	5	19	theme	starch	887:892	arg1	template					894:901	the starch template	883:901	the starch template	883:901	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	2	20	theme	potato	354:359	arg1	waste					361:365	potato waste	354:365	potato waste	354:365	Biopolymers are obtained from natural sources, for instance, starch from potato waste, and its conversion into value-added products is highly significant in a circular economy.
37299358	6	21	theme	aerogels	1086:1093	arg1	functionalization					1045:1061	The functionalization	1041:1061	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials	1041:1132	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	6	22	theme	cryogels	1073:1080	arg1	functionalization					1045:1061	The functionalization	1041:1061	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials	1041:1132	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	0	23	theme	Waste-Based	103:113	arg1	Template					122:129	a Biomass Waste-Based Starch Template	93:129	a Biomass Waste-Based Starch Template	93:129	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	6	24	theme	environmental	1187:1199	arg1	fields					1217:1222	electronic, environmental, and biological fields	1175:1222	electronic, environmental, and biological fields	1175:1222	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	1	25	theme	iron	160:163	arg1	effect					150:155	the effect	146:155	the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template	146:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	2	26	theme	value-added	392:402	arg1	products					404:411	value-added products	392:411	value-added products	392:411	Biopolymers are obtained from natural sources, for instance, starch from potato waste, and its conversion into value-added products is highly significant in a circular economy.
37299358	1	27	theme	biomass	230:236	arg1	template					257:264	a biomass waste-based starch template	228:264	a biomass waste-based starch template	228:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	0	28	theme	Iron	25:28	arg1	Effect					15:20	the Effect	11:20	the Effect of Iron(III) in the Preparation of a Conductive Porous Composite	11:85	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	3	29	theme	iron	584:587	arg1	p-toluenesulfonate					594:611	iron(III) p-toluenesulfonate	584:611	iron(III) p-toluenesulfonate	584:611	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	3	29	theme	iron	584:587	arg1	strategy					618:625	a strategy	616:625	a strategy to functionalize porous biopolymers	616:661	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	3	30	theme	3,4-ethylenedioxythiophene	544:569	arg1	oxidation					531:539	chemical oxidation	522:539	chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers	522:661	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	1	31	theme	waste-based	238:248	arg1	template					257:264	a biomass waste-based starch template	228:264	a biomass waste-based starch template	228:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	2	32	from	significant	423:433	arg1	economy					449:455	a circular economy	438:455	a circular economy	438:455	Biopolymers are obtained from natural sources, for instance, starch from potato waste, and its conversion into value-added products is highly significant in a circular economy.
37299358	3	33	theme	chemical	522:529	arg1	oxidation					531:539	chemical oxidation	522:539	chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers	522:661	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	6	34	theme	porous	1066:1071	arg1	cryogels					1073:1080	porous cryogels	1066:1080	porous cryogels	1066:1080	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	4	35	theme	Thermal	664:670	arg1	properties					716:725	Thermal, spectrophotometric, physical, and chemical properties	664:725	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III),	664:767	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	0	36	theme	Biomass	95:101	arg1	Template					122:129	a Biomass Waste-Based Starch Template	93:129	a Biomass Waste-Based Starch Template	93:129	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	1	37	theme	starch	250:255	arg1	template					257:264	a biomass waste-based starch template	228:264	a biomass waste-based starch template	228:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	0	38	theme	Starch	115:120	arg1	Template					122:129	a Biomass Waste-Based Starch Template	93:129	a Biomass Waste-Based Starch Template	93:129	Evaluating the Effect of Iron(III) in the Preparation of a Conductive Porous Composite Using a Biomass Waste-Based Starch Template.
37299358	1	39	from	effect	150:155	arg1	preparation					177:187	the preparation	173:187	the preparation of a conductive porous composite using a biomass waste-based starch template	173:264	In this work, the effect of iron(III) in the preparation of a conductive porous composite using a biomass waste-based starch template was evaluated.
37299358	5	40	theme	conductive	849:858	arg1	polymer					860:866	the conductive polymer	845:866	the conductive polymer deposited onto the starch template	845:901	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	5	41	theme	composite	978:986	arg1	performance					959:969	the electrical performance	944:969	the electrical performance of the composite	944:986	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	4	42	theme	template	741:748	arg1	properties					716:725	Thermal, spectrophotometric, physical, and chemical properties	664:725	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III),	664:767	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	4	42	theme	template	741:748	arg1	template					741:748	the starch template	730:748	the starch template	730:748	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	4	42	theme	template	741:748	arg1	composites					796:805	the conductive polymer composites	773:805	the conductive polymer composites	773:805	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	5	43	theme	electrical	948:957	arg1	performance					959:969	the electrical performance	944:969	the electrical performance of the composite	944:986	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	4	44	theme	spectrophotometric	673:690	arg1	properties					716:725	Thermal, spectrophotometric, physical, and chemical properties	664:725	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III),	664:767	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	6	45	theme	raw	1120:1122	arg1	polysaccharides					1101:1115	polysaccharides	1101:1115	polysaccharides	1101:1115	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	6	45	theme	raw	1120:1122	arg1	materials					1124:1132	raw materials	1120:1132	raw materials	1120:1132	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	4	46	theme	starch	734:739	arg1	starch/iron					751:761	starch/iron(III)	751:766	starch/iron(III)	751:766	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	4	46	theme	starch	734:739	arg1	template					741:748	the starch template	730:748	the starch template	730:748	Thermal, spectrophotometric, physical, and chemical properties of the starch template, starch/iron(III), and the conductive polymer composites were evaluated.
37299358	5	47	theme	polymer	860:866	arg1	data					837:840	The impedance data	823:840	The impedance data of the conductive polymer deposited onto the starch template	823:901	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	2	48	from	economy	449:455	arg1	significant					423:433	significant	423:433	significant	423:433	Biopolymers are obtained from natural sources, for instance, starch from potato waste, and its conversion into value-added products is highly significant in a circular economy.
37299358	2	49	theme	natural	311:317	arg1	sources					319:325	natural sources	311:325	natural sources	311:325	Biopolymers are obtained from natural sources, for instance, starch from potato waste, and its conversion into value-added products is highly significant in a circular economy.
37299358	5	50	theme	longer	923:928	arg1	time					938:941	a longer soaking time	921:941	a longer soaking time	921:941	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	3	51	theme	porous	644:649	arg1	biopolymers					651:661	porous biopolymers	644:661	porous biopolymers	644:661	The biomass starch-based conductive cryogel was polymerized via chemical oxidation of 3,4-ethylenedioxythiophene (EDOT) using iron(III) p-toluenesulfonate as a strategy to functionalize porous biopolymers.
37299358	6	52	theme	great	1140:1144	arg1	interest					1146:1153	great interest	1140:1153	great interest for applications in electronic, environmental, and biological fields	1140:1222	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
37299358	5	53	theme	impedance	827:835	arg1	data					837:840	The impedance data	823:840	The impedance data of the conductive polymer deposited onto the starch template	823:901	The impedance data of the conductive polymer deposited onto the starch template confirmed that at a longer soaking time, the electrical performance of the composite was improved, slightly modifying its microstructure.
37299358	6	54	from	interest	1146:1153	arg1	fields					1217:1222	electronic, environmental, and biological fields	1175:1222	electronic, environmental, and biological fields	1175:1222	The functionalization of porous cryogels and aerogels using polysaccharides as raw materials is of great interest for applications in electronic, environmental, and biological fields.
36115268	7	0	theme	adaptive	1149:1156	arg1	significance					1158:1169	The adaptive significance	1145:1169	The adaptive significance of these changes	1145:1186	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	1	1	theme	great	150:154	arg1	platform					156:163	a great platform	148:163	a great platform for researching nanoparticles effects on living organisms including plants	148:238	Nanotechnologies provide a great platform for researching nanoparticles effects on living organisms including plants.
36115268	5	2	theme	chlorophylls	898:909	arg1	content					887:893	the content	883:893	the content of chlorophylls	883:909	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	2	theme	chlorophylls	898:909	arg1	intensity					854:862	The intensity	850:862	The intensity of photosynthesis	850:880	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	2	theme	chlorophylls	898:909	arg1	portion					920:926	the portion	916:926	the portion of unsaturated fatty acids in the composition of total leaf lipids	916:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	4	3	from	grains	780:785	arg1	size					755:758	the size	751:758	the size of plastids	751:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	3	from	grains	780:785	arg1	grana					843:847	grana	843:847	grana	843:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	3	from	grains	780:785	arg1	chloroplasts					797:808	chloroplasts	797:808	chloroplasts	797:808	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	8	4	theme	first	1300:1304	arg1	This					1288:1291	This	1288:1291	This	1288:1291	This is the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance.
36115268	8	4	theme	first	1300:1304	arg1	report					1306:1311	the first report	1296:1311	the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance	1296:1402	This is the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance.
36115268	8	5	theme	new	1349:1351	arg1	nanopriming					1321:1331	nanopriming	1321:1331	nanopriming with AuNPs	1321:1342	This is the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance.
36115268	8	5	theme	new	1349:1351	arg1	method					1353:1358	a new method	1347:1358	a new method to study the mechanisms of stress tolerance	1347:1402	This is the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance.
36115268	7	6	from	perspectives	1251:1262	arg1	plants					1233:1238	plants	1233:1238	plants	1233:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	3	7	dep	AuNPs	477:481	arg1	concentration					517:529	concentration	517:529	concentration of 20 μg ml-1	517:543	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	3	7	dep	AuNPs	477:481	arg1	diameter					496:503	the average diameter	484:503	the average diameter of 15.3 nm	484:514	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	5	8	theme	lipids	988:993	arg1	composition					962:972	the composition	958:972	the composition of total leaf lipids	958:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	9	from	content	887:893	arg1	composition					962:972	the composition	958:972	the composition of total leaf lipids	958:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	2	10	dep	Triticum	359:366	arg1	aestivum					368:375	Triticum aestivum	359:375	Triticum aestivum seedlings	359:385	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	4	11	theme	transmission	669:680	arg1	microscopy					691:700	transmission electron microscopy	669:700	transmission electron microscopy in plants grown from treated seeds	669:735	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	7	12	theme	possible	1189:1196	arg1	mechanisms					1198:1207	possible mechanisms	1189:1207	possible mechanisms of the AuNPs effects on plants	1189:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	4	13	from	grana	843:847	arg1	number					819:824	the number	815:824	the number of thylakoids in grana	815:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	13	from	grana	843:847	arg1	grains					780:785	starch grains	773:785	starch grains	773:785	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	13	from	grana	843:847	arg1	grana					788:792	grana	788:792	grana in chloroplasts	788:808	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	13	from	grana	843:847	arg1	increases					738:746	increases	738:746	increases in the size of plastids	738:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	14	theme	plastids	763:770	arg1	size					755:758	the size	751:758	the size of plastids	751:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	7	15	from	plants	1233:1238	arg1	perspectives					1251:1262	future perspectives	1244:1262	future perspectives of study	1244:1271	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	15	from	plants	1233:1238	arg1	significance					1158:1169	The adaptive significance	1145:1169	The adaptive significance of these changes	1145:1186	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	15	from	plants	1233:1238	arg1	mechanisms					1198:1207	possible mechanisms	1189:1207	possible mechanisms of the AuNPs effects on plants	1189:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	2	16	theme	priming	288:294	arg1	effect					273:278	the stimulating effect	257:278	the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings	257:385	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	4	17	from	microscopy	691:700	arg1	plants					705:710	plants	705:710	plants grown from treated seeds	705:735	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	0	18	from	changes	41:47	arg1	apparatus					71:79	the photosynthetic apparatus	52:79	the photosynthetic apparatus	52:79	Priming with gold nanoparticles leads to changes in the photosynthetic apparatus and improves the cold tolerance of wheat.
36115268	4	19	theme	thylakoids	829:838	arg1	number					819:824	the number	815:824	the number of thylakoids in grana	815:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	19	theme	thylakoids	829:838	arg1	grains					780:785	starch grains	773:785	starch grains	773:785	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	19	theme	thylakoids	829:838	arg1	grana					788:792	grana	788:792	grana in chloroplasts	788:808	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	19	theme	thylakoids	829:838	arg1	increases					738:746	increases	738:746	increases in the size of plastids	738:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	2	20	theme	seed	283:286	arg1	priming					288:294	seed priming	283:294	seed priming with gold nanoparticles (AuNPs)	283:326	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	3	21	theme	mass	454:457	arg1	spectrometry					459:470	inductively coupled plasma-atomic emission and mass spectrometry	407:470	spectrometry	459:470	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	0	22	theme	cold	98:101	arg1	tolerance					103:111	the cold tolerance	94:111	the cold tolerance of wheat	94:120	Priming with gold nanoparticles leads to changes in the photosynthetic apparatus and improves the cold tolerance of wheat.
36115268	4	23	from	thylakoids	829:838	arg1	grana					843:847	grana	843:847	grana	843:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	5	24	theme	unsaturated	931:941	arg1	acids					949:953	unsaturated fatty acids	931:953	unsaturated fatty acids in the composition of total leaf lipids	931:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	4	25	theme	starch	773:778	arg1	grains					780:785	starch grains	773:785	starch grains	773:785	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	5	26	from	composition	962:972	arg1	content					887:893	the content	883:893	the content of chlorophylls	883:909	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	26	from	composition	962:972	arg1	intensity					854:862	The intensity	850:862	The intensity of photosynthesis	850:880	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	26	from	composition	962:972	arg1	portion					920:926	the portion	916:926	the portion of unsaturated fatty acids in the composition of total leaf lipids	916:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	1	27	theme	researching	169:179	arg1	effects					195:201	researching nanoparticles effects	169:201	researching nanoparticles effects on living organisms including plants	169:238	Nanotechnologies provide a great platform for researching nanoparticles effects on living organisms including plants.
36115268	7	28	from	effects	1222:1228	arg1	plants					1233:1238	plants	1233:1238	plants	1233:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	4	29	from	changes	628:634	arg1	chloroplasts					639:650	chloroplasts	639:650	chloroplasts	639:650	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	1	30	theme	nanoparticles	181:193	arg1	effects					195:201	researching nanoparticles effects	169:201	researching nanoparticles effects on living organisms including plants	169:238	Nanotechnologies provide a great platform for researching nanoparticles effects on living organisms including plants.
36115268	7	31	from	mechanisms	1198:1207	arg1	plants					1233:1238	plants	1233:1238	plants	1233:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	3	32	dep	seedling	595:602	arg1	leaves					604:609	leaves	604:609	seedling leaves	595:609	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	0	33	theme	wheat	116:120	arg1	tolerance					103:111	the cold tolerance	94:111	the cold tolerance of wheat	94:120	Priming with gold nanoparticles leads to changes in the photosynthetic apparatus and improves the cold tolerance of wheat.
36115268	6	34	theme	low	1128:1130	arg1	temperature					1132:1142	low temperature	1128:1142	low temperature	1128:1142	This study demonstrates that revealed changes determined the increased tolerance of wheat to low temperature.
36115268	4	35	dep	found	657:661	arg1	grains					780:785	starch grains	773:785	starch grains	773:785	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	35	dep	found	657:661	arg1	number					819:824	the number	815:824	the number of thylakoids in grana	815:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	35	dep	found	657:661	arg1	grana					788:792	grana	788:792	grana in chloroplasts	788:808	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	35	dep	found	657:661	arg1	increases					738:746	increases	738:746	increases in the size of plastids	738:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	5	36	theme	fatty	943:947	arg1	acids					949:953	unsaturated fatty acids	931:953	unsaturated fatty acids in the composition of total leaf lipids	931:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	0	37	theme	gold	13:16	arg1	nanoparticles					18:30	gold nanoparticles	13:30	gold nanoparticles	13:30	Priming with gold nanoparticles leads to changes in the photosynthetic apparatus and improves the cold tolerance of wheat.
36115268	2	38	theme	gold	301:304	arg1	nanoparticles					306:318	gold nanoparticles	301:318	gold nanoparticles (AuNPs)	301:326	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	2	38	theme	gold	301:304	arg1	AuNPs					321:325	AuNPs	321:325	AuNPs	321:325	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	2	39	from	effect	273:278	arg1	apparatus					346:354	photosynthetic apparatus	331:354	photosynthetic apparatus of Triticum aestivum seedlings	331:385	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	1	40	theme	living	206:211	arg1	plants					233:238	plants	233:238	plants	233:238	Nanotechnologies provide a great platform for researching nanoparticles effects on living organisms including plants.
36115268	1	40	theme	living	206:211	arg1	organisms					213:221	living organisms	206:221	living organisms including plants	206:238	Nanotechnologies provide a great platform for researching nanoparticles effects on living organisms including plants.
36115268	3	41	theme	coupled	419:425	arg1	emission					441:448	inductively coupled plasma-atomic emission and mass spectrometry	407:470	emission	441:448	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	3	42	theme	15.3 nm	508:514	arg1	concentration					517:529	concentration	517:529	concentration of 20 μg ml-1	517:543	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	3	42	theme	15.3 nm	508:514	arg1	diameter					496:503	the average diameter	484:503	the average diameter of 15.3 nm	484:514	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	3	43	dep	emission	441:448	arg1	found					586:590	found	586:590	were not found in seedling leaves	577:609	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	3	43	dep	emission	441:448	arg1	penetrated					546:555	penetrated	546:555	penetrated into the seeds	546:570	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	4	44	theme	electron	682:689	arg1	microscopy					691:700	transmission electron microscopy	669:700	transmission electron microscopy in plants grown from treated seeds	669:735	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	3	45	theme	plasma-atomic	427:439	arg1	emission					441:448	inductively coupled plasma-atomic emission and mass spectrometry	407:470	emission	441:448	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	2	46	theme	Triticum	359:366	arg1	seedlings					377:385	Triticum aestivum seedlings	359:385	Triticum aestivum seedlings	359:385	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	4	47	theme	treated	723:729	arg1	seeds					731:735	treated seeds	723:735	treated seeds	723:735	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	48	from	number	819:824	arg1	size					755:758	the size	751:758	the size of plastids	751:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	48	from	number	819:824	arg1	grana					843:847	grana	843:847	grana	843:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	48	from	number	819:824	arg1	chloroplasts					797:808	chloroplasts	797:808	chloroplasts	797:808	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	7	49	theme	changes	1180:1186	arg1	perspectives					1251:1262	future perspectives	1244:1262	future perspectives of study	1244:1271	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	49	theme	changes	1180:1186	arg1	significance					1158:1169	The adaptive significance	1145:1169	The adaptive significance of these changes	1145:1186	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	49	theme	changes	1180:1186	arg1	mechanisms					1198:1207	possible mechanisms	1189:1207	possible mechanisms of the AuNPs effects on plants	1189:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	5	50	theme	photosynthesis	867:880	arg1	content					887:893	the content	883:893	the content of chlorophylls	883:909	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	50	theme	photosynthesis	867:880	arg1	intensity					854:862	The intensity	850:862	The intensity of photosynthesis	850:880	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	50	theme	photosynthesis	867:880	arg1	portion					920:926	the portion	916:926	the portion of unsaturated fatty acids in the composition of total leaf lipids	916:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	51	theme	acids	949:953	arg1	content					887:893	the content	883:893	the content of chlorophylls	883:909	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	51	theme	acids	949:953	arg1	intensity					854:862	The intensity	850:862	The intensity of photosynthesis	850:880	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	51	theme	acids	949:953	arg1	portion					920:926	the portion	916:926	the portion of unsaturated fatty acids in the composition of total leaf lipids	916:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	7	52	from	significance	1158:1169	arg1	plants					1233:1238	plants	1233:1238	plants	1233:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	6	53	theme	wheat	1119:1123	arg1	tolerance					1106:1114	the increased tolerance	1092:1114	the increased tolerance of wheat to low temperature	1092:1142	This study demonstrates that revealed changes determined the increased tolerance of wheat to low temperature.
36115268	5	54	theme	treated	1013:1019	arg1	plants					1027:1032	treated AuNPs plants	1013:1032	treated AuNPs plants	1013:1032	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	8	55	theme	tolerance	1394:1402	arg1	mechanisms					1373:1382	the mechanisms	1369:1382	the mechanisms of stress tolerance	1369:1402	This is the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance.
36115268	2	56	theme	photosynthetic	331:344	arg1	apparatus					346:354	photosynthetic apparatus	331:354	photosynthetic apparatus of Triticum aestivum seedlings	331:385	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	4	57	from	increases	738:746	arg1	size					755:758	the size	751:758	the size of plastids	751:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	57	from	increases	738:746	arg1	grana					843:847	grana	843:847	grana	843:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	57	from	increases	738:746	arg1	chloroplasts					797:808	chloroplasts	797:808	chloroplasts	797:808	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	5	58	theme	leaf	983:986	arg1	lipids					988:993	total leaf lipids	977:993	total leaf lipids	977:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	59	theme	AuNPs	1021:1025	arg1	plants					1027:1032	treated AuNPs plants	1013:1032	treated AuNPs plants	1013:1032	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	8	60	theme	stress	1387:1392	arg1	tolerance					1394:1402	stress tolerance	1387:1402	stress tolerance	1387:1402	This is the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance.
36115268	0	61	with	Priming	0:6	arg1	nanoparticles					18:30	gold nanoparticles	13:30	gold nanoparticles	13:30	Priming with gold nanoparticles leads to changes in the photosynthetic apparatus and improves the cold tolerance of wheat.
36115268	3	62	theme	average	488:494	arg1	concentration					517:529	concentration	517:529	concentration of 20 μg ml-1	517:543	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	3	62	theme	average	488:494	arg1	diameter					496:503	the average diameter	484:503	the average diameter of 15.3 nm	484:514	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	7	63	theme	study	1267:1271	arg1	perspectives					1251:1262	future perspectives	1244:1262	future perspectives of study	1244:1271	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	63	theme	study	1267:1271	arg1	significance					1158:1169	The adaptive significance	1145:1169	The adaptive significance of these changes	1145:1186	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	63	theme	study	1267:1271	arg1	mechanisms					1198:1207	possible mechanisms	1189:1207	possible mechanisms of the AuNPs effects on plants	1189:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	2	64	theme	stimulating	261:271	arg1	effect					273:278	the stimulating effect	257:278	the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings	257:385	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	5	65	from	intensity	854:862	arg1	composition					962:972	the composition	958:972	the composition of total leaf lipids	958:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	6	66	theme	increased	1096:1104	arg1	tolerance					1106:1114	the increased tolerance	1092:1114	the increased tolerance of wheat to low temperature	1092:1142	This study demonstrates that revealed changes determined the increased tolerance of wheat to low temperature.
36115268	7	67	theme	future	1244:1249	arg1	perspectives					1251:1262	future perspectives	1244:1262	future perspectives of study	1244:1271	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	0	68	theme	photosynthetic	56:69	arg1	apparatus					71:79	the photosynthetic apparatus	52:79	the photosynthetic apparatus	52:79	Priming with gold nanoparticles leads to changes in the photosynthetic apparatus and improves the cold tolerance of wheat.
36115268	5	69	from	acids	949:953	arg1	composition					962:972	the composition	958:972	the composition of total leaf lipids	958:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	5	70	theme	total	977:981	arg1	lipids					988:993	total leaf lipids	977:993	total leaf lipids	977:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	1	71	from	effects	195:201	arg1	plants					233:238	plants	233:238	plants	233:238	Nanotechnologies provide a great platform for researching nanoparticles effects on living organisms including plants.
36115268	1	71	from	effects	195:201	arg1	organisms					213:221	living organisms	206:221	living organisms including plants	206:238	Nanotechnologies provide a great platform for researching nanoparticles effects on living organisms including plants.
36115268	2	72	theme	seedlings	377:385	arg1	apparatus					346:354	photosynthetic apparatus	331:354	photosynthetic apparatus of Triticum aestivum seedlings	331:385	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	5	73	from	portion	920:926	arg1	composition					962:972	the composition	958:972	the composition of total leaf lipids	958:993	The intensity of photosynthesis, the content of chlorophylls, and the portion of unsaturated fatty acids in the composition of total leaf lipids were increased in treated AuNPs plants.
36115268	8	74	with	nanopriming	1321:1331	arg1	AuNPs					1338:1342	AuNPs	1338:1342	AuNPs	1338:1342	This is the first report showing nanopriming with AuNPs as a new method to study the mechanisms of stress tolerance.
36115268	7	75	theme	AuNPs	1216:1220	arg1	effects					1222:1228	the AuNPs effects	1212:1228	the AuNPs effects on plants	1212:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	4	76	theme	Ultrastructural	612:626	arg1	changes					628:634	Ultrastructural changes	612:634	Ultrastructural changes in chloroplasts	612:650	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	77	from	grana	788:792	arg1	size					755:758	the size	751:758	the size of plastids	751:770	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	77	from	grana	788:792	arg1	grana					843:847	grana	843:847	grana	843:847	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	4	77	from	grana	788:792	arg1	chloroplasts					797:808	chloroplasts	797:808	chloroplasts	797:808	Ultrastructural changes in chloroplasts were found using transmission electron microscopy in plants grown from treated seeds: increases in the size of plastids, starch grains, grana in chloroplasts, and the number of thylakoids in grana.
36115268	3	78	theme	20 μg ml-1	534:543	arg1	concentration					517:529	concentration	517:529	concentration of 20 μg ml-1	517:543	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	3	78	theme	20 μg ml-1	534:543	arg1	diameter					496:503	the average diameter	484:503	the average diameter of 15.3 nm	484:514	It was found using inductively coupled plasma-atomic emission and mass spectrometry that AuNPs (the average diameter of 15.3 nm, concentration of 20 μg ml-1) penetrated into the seeds, but were not found in seedling leaves.
36115268	7	79	theme	effects	1222:1228	arg1	perspectives					1251:1262	future perspectives	1244:1262	future perspectives of study	1244:1271	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	79	theme	effects	1222:1228	arg1	significance					1158:1169	The adaptive significance	1145:1169	The adaptive significance of these changes	1145:1186	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	7	79	theme	effects	1222:1228	arg1	mechanisms					1198:1207	possible mechanisms	1189:1207	possible mechanisms of the AuNPs effects on plants	1189:1238	The adaptive significance of these changes, possible mechanisms of the AuNPs effects on plants and future perspectives of study are discussed.
36115268	2	80	with	priming	288:294	arg1	nanoparticles					306:318	gold nanoparticles	301:318	gold nanoparticles (AuNPs)	301:326	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
36115268	2	80	with	priming	288:294	arg1	AuNPs					321:325	AuNPs	321:325	AuNPs	321:325	This work shows the stimulating effect of seed priming with gold nanoparticles (AuNPs) on photosynthetic apparatus of Triticum aestivum seedlings.
37156313	8	0	theme	%	1157:1157	arg1	cytotoxicity					1159:1170	<5 % cytotoxicity	1154:1170	<5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A)	1154:1221	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	10	1	from	increase	1595:1602	arg1	genes					1784:1788	metastasis-enhancing genes	1763:1788	metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9)	1763:1813	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	1	from	increase	1595:1602	arg1	expression					1611:1620	expression	1611:1620	expression	1611:1620	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	1	from	increase	1595:1602	arg1	MMP-9					1808:1812	MMP-9	1808:1812	MMP-9	1808:1812	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	1	from	increase	1595:1602	arg1	activity					1626:1633	activity	1626:1633	activity	1626:1633	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	1	from	increase	1595:1602	arg1	Bcl2					1791:1794	Bcl2	1791:1794	Bcl2	1791:1794	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	1	from	increase	1595:1602	arg1	MMP-2					1797:1801	MMP-2	1797:1801	MMP-2	1797:1801	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	4	2	theme	@	646:646	arg1	niosomes					629:636	paclitaxel-loaded niosomes	611:636	paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	611:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	4	2	theme	@	646:646	arg1	GT-AL					647:651	Nio-PTX@GT-AL	639:651	Nio-PTX@GT-AL	639:651	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	5	3	theme	cellular	698:705	arg1	uptake					707:712	cellular uptake	698:712	cellular uptake	698:712	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	2	4	theme	metastasis	309:318	arg1	effects					276:282	side effects	271:282	side effects of anti-cancer drugs and metastasis	271:318	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	2	4	theme	metastasis	309:318	arg1	challenges					339:348	the main challenges	330:348	the main challenges in breast cancer treatment	330:375	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	8	5	theme	breast	1196:1201	arg1	MCF-10A					1214:1220	MCF-10A	1214:1220	MCF-10A	1214:1220	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	8	5	theme	breast	1196:1201	arg1	line					1208:1211	non-tumorigenic breast cell line	1180:1211	non-tumorigenic breast cell line (MCF-10A)	1180:1221	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	6	6	theme	synthesized	902:912	arg1	niosomes					914:921	synthesized niosomes	902:921	synthesized niosomes	902:921	Results demonstrated that synthesized niosomes had spherical-like, in the range of 60-80 nm with desirable cellular uptake.
37156313	10	7	theme	genes	1638:1642	arg1	expression					1611:1620	expression	1611:1620	expression	1611:1620	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	7	theme	genes	1638:1642	arg1	activity					1626:1633	activity	1626:1633	activity	1626:1633	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	6	8	contain	had	923:925	arg2	spherical-like					927:940	spherical-like	927:940	spherical-like	927:940	Results demonstrated that synthesized niosomes had spherical-like, in the range of 60-80 nm with desirable cellular uptake.
37156313	6	8	contain	had	923:925	arg1	niosomes					914:921	synthesized niosomes	902:921	synthesized niosomes	902:921	Results demonstrated that synthesized niosomes had spherical-like, in the range of 60-80 nm with desirable cellular uptake.
37156313	10	9	theme	expression	1552:1561	arg1	regulation					1563:1572	gene expression regulation	1547:1572	gene expression regulation	1547:1572	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	2	10	theme	drugs	299:303	arg1	effects					276:282	side effects	271:282	side effects of anti-cancer drugs and metastasis	271:318	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	2	10	theme	drugs	299:303	arg1	challenges					339:348	the main challenges	330:348	the main challenges in breast cancer treatment	330:375	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	1	11	theme	lethal	212:217	arg1	cancers					219:225	the most lethal cancers	203:225	the most lethal cancers	203:225	Breast cancer is one of the most lethal cancers, especially in women.
37156313	8	12	theme	breast	1260:1265	arg1	cancer					1267:1272	breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples	1260:1363	cancer	1267:1272	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	2	13	theme	main	334:337	arg1	effects					276:282	side effects	271:282	side effects of anti-cancer drugs and metastasis	271:318	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	2	13	theme	main	334:337	arg1	challenges					339:348	the main challenges	330:348	the main challenges in breast cancer treatment	330:375	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	9	14	theme	surface	1447:1453	arg1	area					1455:1458	covered surface area	1439:1458	covered surface area	1439:1458	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	6	15	theme	cellular	983:990	arg1	uptake					992:997	desirable cellular uptake	973:997	desirable cellular uptake	973:997	Results demonstrated that synthesized niosomes had spherical-like, in the range of 60-80 nm with desirable cellular uptake.
37156313	6	16	with	60-80 nm	959:966	arg1	uptake					992:997	desirable cellular uptake	973:997	desirable cellular uptake	973:997	Results demonstrated that synthesized niosomes had spherical-like, in the range of 60-80 nm with desirable cellular uptake.
37156313	5	17	theme	cell	731:734	arg1	cytotoxicity					736:747	cell cytotoxicity	731:747	cell cytotoxicity	731:747	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	8	18	contain	had	1150:1152	arg1	scaffold					1141:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	8	18	contain	had	1150:1152	arg2	cytotoxicity					1159:1170	<5 % cytotoxicity	1154:1170	<5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A)	1154:1221	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	10	19	dep	genes	1784:1788	arg1	MMP-9					1808:1812	MMP-9	1808:1812	MMP-9	1808:1812	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	19	dep	genes	1784:1788	arg1	Bcl2					1791:1794	Bcl2	1791:1794	Bcl2	1791:1794	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	19	dep	genes	1784:1788	arg1	genes					1784:1788	metastasis-enhancing genes	1763:1788	metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9)	1763:1813	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	19	dep	genes	1784:1788	arg1	MMP-2					1797:1801	MMP-2	1797:1801	MMP-2	1797:1801	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	20	theme	metastasis-enhancing	1763:1782	arg1	MMP-9					1808:1812	MMP-9	1808:1812	MMP-9	1808:1812	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	20	theme	metastasis-enhancing	1763:1782	arg1	Bcl2					1791:1794	Bcl2	1791:1794	Bcl2	1791:1794	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	20	theme	metastasis-enhancing	1763:1782	arg1	genes					1784:1788	metastasis-enhancing genes	1763:1788	metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9)	1763:1813	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	20	theme	metastasis-enhancing	1763:1782	arg1	MMP-2					1797:1801	MMP-2	1797:1801	MMP-2	1797:1801	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	8	21	theme	80 	1234:1236	arg1	%					1237:1237	%	1237:1237	%	1237:1237	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	5	22	theme	flow	715:718	arg1	cytometry					720:728	flow cytometry	715:728	flow cytometry	715:728	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	9	23	dep	%	1424:1424	arg1	70 					1421:1423	70 	1421:1423	70 	1421:1423	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	7	24	theme	drug	1042:1045	arg1	release					1047:1053	a sustained drug release	1030:1053	a sustained drug release	1030:1053	Nio-PTX@GT-AL and Nio-PTX had a sustained drug release and were biodegradable.
37156313	7	25	theme	@	1007:1007	arg1	GT-AL					1008:1012	Nio-PTX@GT-AL	1000:1012	Nio-PTX@GT-AL	1000:1012	Nio-PTX@GT-AL and Nio-PTX had a sustained drug release and were biodegradable.
37156313	0	26	theme	gelatin/alginate	92:107	arg1	composite					109:117	cross-linked gelatin/alginate composite	79:117	cross-linked gelatin/alginate composite	79:117	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	2	27	theme	side	271:274	arg1	effects					276:282	side effects	271:282	side effects of anti-cancer drugs and metastasis	271:318	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	2	27	theme	side	271:274	arg1	challenges					339:348	the main challenges	330:348	the main challenges in breast cancer treatment	330:375	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	2	28	from	challenges	339:348	arg1	treatment					367:375	breast cancer treatment	353:375	breast cancer treatment	353:375	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	4	29	theme	drug	532:535	arg1	system					546:551	an advanced drug delivery system	520:551	an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	520:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	8	30	theme	control	1349:1355	arg1	samples					1357:1363	control samples	1349:1363	control samples	1349:1363	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	11	31	theme	cytometry	1841:1849	arg1	results					1851:1857	flow cytometry results	1836:1857	flow cytometry results	1836:1857	Also, flow cytometry results declared that Nio-PTX@GT-AL reduced necrosis and increased apoptosis considerably.
37156313	0	32	theme	in-vitro	146:153	arg1	evaluation					167:176	in-vitro anti-cancer evaluation	146:176	in-vitro anti-cancer evaluation	146:176	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	0	33	dep	synthesis	132:140	arg1	synthesis					21:29	3D-printing-assisted synthesis	0:29	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite	0:117	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	3	34	theme	new	462:464	arg1	horizons					466:473	new horizons	462:473	new horizons	462:473	Recently, advanced technologies such as 3D-printing and nanotechnology have created new horizons in cancer treatment.
37156313	0	35	theme	3D-printing-assisted	0:19	arg1	synthesis					21:29	3D-printing-assisted synthesis	0:29	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite	0:117	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	4	36	theme	gelatin-alginate	573:588	arg1	scaffolds					590:598	3D-printed gelatin-alginate scaffolds	562:598	3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	562:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	11	37	theme	Nio-PTX	1873:1879	arg1	GT-AL					1881:1885	Nio-PTX@GT-AL	1873:1885	Nio-PTX@GT-AL	1873:1885	Also, flow cytometry results declared that Nio-PTX@GT-AL reduced necrosis and increased apoptosis considerably.
37156313	10	38	theme	anticancer	1478:1487	arg1	effect					1489:1494	The anticancer effect	1474:1494	The anticancer effect of the designed nanocarrier	1474:1522	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	0	39	theme	niosomes	52:59	arg1	synthesis					21:29	3D-printing-assisted synthesis	0:29	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite	0:117	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	5	40	theme	scaffolds	802:810	arg1	morphology					659:668	The morphology	655:668	The morphology	655:668	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	cytotoxicity					736:747	cell cytotoxicity	731:747	cell cytotoxicity	731:747	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	Free-PTX					847:854	Free-PTX	847:854	Free-PTX	847:854	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	uptake					707:712	cellular uptake	698:712	cellular uptake	698:712	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	release					676:682	drug release	671:682	drug release	671:682	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	activity					790:797	caspase activity	782:797	caspase activity	782:797	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	samples					825:831	control samples	817:831	control samples	817:831	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	migration					750:758	migration	750:758	migration	750:758	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	cytometry					720:728	flow cytometry	715:728	flow cytometry	715:728	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	Nio-PTX					834:840	Nio-PTX	834:840	Nio-PTX	834:840	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	degradation					685:695	degradation	685:695	degradation	685:695	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	40	theme	scaffolds	802:810	arg1	expression					766:775	gene expression	761:775	gene expression	761:775	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	7	41	contain	had	1026:1028	arg1	Nio-PTX					1018:1024	Nio-PTX	1018:1024	Nio-PTX	1018:1024	Nio-PTX@GT-AL and Nio-PTX had a sustained drug release and were biodegradable.
37156313	7	41	contain	had	1026:1028	arg1	GT-AL					1008:1012	Nio-PTX@GT-AL	1000:1012	Nio-PTX@GT-AL	1000:1012	Nio-PTX@GT-AL and Nio-PTX had a sustained drug release and were biodegradable.
37156313	7	41	contain	had	1026:1028	arg2	release					1047:1053	a sustained drug release	1030:1053	a sustained drug release	1030:1053	Nio-PTX@GT-AL and Nio-PTX had a sustained drug release and were biodegradable.
37156313	9	42	dep	reduction	1426:1434	arg1	%					1424:1424	%	1424:1424	%	1424:1424	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	8	43	theme	designed	1118:1125	arg1	scaffold					1141:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	10	44	theme	designed	1503:1510	arg1	nanocarrier					1512:1522	the designed nanocarrier	1499:1522	the designed nanocarrier	1499:1522	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	2	45	theme	breast	353:358	arg1	treatment					367:375	breast cancer treatment	353:375	breast cancer treatment	353:375	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	8	46	theme	@	1134:1134	arg1	scaffold					1141:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	10	47	theme	gene	1547:1550	arg1	regulation					1563:1572	gene expression regulation	1547:1572	gene expression regulation	1547:1572	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	48	dep	apoptosis	1654:1662	arg1	CASP-9					1685:1690	CASP-9	1685:1690	CASP-9	1685:1690	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	48	dep	apoptosis	1654:1662	arg1	CASP-3					1665:1670	CASP-3	1665:1670	CASP-3	1665:1670	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	48	dep	apoptosis	1654:1662	arg1	CASP-8					1673:1678	CASP-8	1673:1678	CASP-8	1673:1678	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	12	49	theme	efficient	2081:2089	arg1	delivery					2096:2103	efficient drug delivery	2081:2103	efficient drug delivery applications	2081:2116	The results of this study prove that employing 3D-printing and niosomal formulation is an effective approach in designing nanocarriers for efficient drug delivery applications.
37156313	12	50	theme	study	1962:1966	arg1	results					1946:1952	The results	1942:1952	The results of this study	1942:1966	The results of this study prove that employing 3D-printing and niosomal formulation is an effective approach in designing nanocarriers for efficient drug delivery applications.
37156313	5	51	theme	caspase	782:788	arg1	activity					790:797	caspase activity	782:797	caspase activity	782:797	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	10	52	dep	expression	1611:1620	arg1	the					1607:1609	the	1607:1609	the	1607:1609	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	53	theme	inhibiting	1697:1706	arg1	metastasis					1708:1717	inhibiting metastasis	1697:1717	inhibiting metastasis (Bax, and p53)	1697:1732	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	8	54	theme	<5 	1154:1156	arg1	%					1157:1157	%	1157:1157	%	1157:1157	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	10	55	theme	remarkable	1740:1749	arg1	decrease					1751:1758	a remarkable decrease	1738:1758	a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9)	1738:1813	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	4	56	theme	Nio-PTX	639:645	arg1	niosomes					629:636	paclitaxel-loaded niosomes	611:636	paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	611:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	4	56	theme	Nio-PTX	639:645	arg1	GT-AL					647:651	Nio-PTX@GT-AL	639:651	Nio-PTX@GT-AL	639:651	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	9	57	theme	covered	1439:1445	arg1	area					1455:1458	covered surface area	1439:1458	covered surface area	1439:1458	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	1	58	theme	most	207:210	arg1	cancers					219:225	the most lethal cancers	203:225	the most lethal cancers	203:225	Breast cancer is one of the most lethal cancers, especially in women.
37156313	8	59	theme	non-tumorigenic	1180:1194	arg1	MCF-10A					1214:1220	MCF-10A	1214:1220	MCF-10A	1214:1220	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	8	59	theme	non-tumorigenic	1180:1194	arg1	line					1208:1211	non-tumorigenic breast cell line	1180:1211	non-tumorigenic breast cell line (MCF-10A)	1180:1221	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	0	60	link	cross-linked	79:90	arg1	composite					109:117	cross-linked gelatin/alginate composite	79:117	cross-linked gelatin/alginate composite	79:117	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	8	61	theme	%	1237:1237	arg1	cytotoxicity					1239:1250	80 % cytotoxicity	1234:1250	80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples	1234:1363	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	5	62	theme	drug	671:674	arg1	release					676:682	drug release	671:682	drug release	671:682	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	4	63	contain	containing	600:609	arg1	scaffolds					590:598	3D-printed gelatin-alginate scaffolds	562:598	3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	562:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	4	63	contain	containing	600:609	arg2	GT-AL					647:651	Nio-PTX@GT-AL	639:651	Nio-PTX@GT-AL	639:651	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	4	63	contain	containing	600:609	arg2	niosomes					629:636	paclitaxel-loaded niosomes	611:636	paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	611:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	1	64	theme	cancers	219:225	arg1	cancers					219:225	the most lethal cancers	203:225	the most lethal cancers	203:225	Breast cancer is one of the most lethal cancers, especially in women.
37156313	1	64	theme	cancers	219:225	arg1	one					196:198	one	196:198	one	196:198	Breast cancer is one of the most lethal cancers, especially in women.
37156313	8	65	theme	cell	1203:1206	arg1	MCF-10A					1214:1220	MCF-10A	1214:1220	MCF-10A	1214:1220	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	8	65	theme	cell	1203:1206	arg1	line					1208:1211	non-tumorigenic breast cell line	1180:1211	non-tumorigenic breast cell line (MCF-10A)	1180:1221	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	9	66	located	observed	1464:1471	arg1	scratch-assay					1391:1403	scratch-assay	1391:1403	scratch-assay	1391:1403	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	9	66	located	observed	1464:1471	arg2	reduction					1426:1434	approximately 70 % reduction	1407:1434	approximately 70 % reduction of covered surface area	1407:1458	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	9	66	located	observed	1464:1471	arg1	evaluation					1379:1388	migration evaluation	1369:1388	migration evaluation (scratch-assay)	1369:1404	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	12	67	theme	delivery	2096:2103	arg1	applications					2105:2116	efficient drug delivery applications	2081:2116	efficient drug delivery applications	2081:2116	The results of this study prove that employing 3D-printing and niosomal formulation is an effective approach in designing nanocarriers for efficient drug delivery applications.
37156313	10	68	theme	significant	1583:1593	arg1	increase					1595:1602	a significant increase	1581:1602	a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53)	1581:1732	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	9	69	theme	area	1455:1458	arg1	reduction					1426:1434	approximately 70 % reduction	1407:1434	approximately 70 % reduction of covered surface area	1407:1458	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	6	70	theme	desirable	973:981	arg1	uptake					992:997	desirable cellular uptake	973:997	desirable cellular uptake	973:997	Results demonstrated that synthesized niosomes had spherical-like, in the range of 60-80 nm with desirable cellular uptake.
37156313	6	71	theme	60-80 nm	959:966	arg1	range					950:954	the range	946:954	the range of 60-80 nm with desirable cellular uptake	946:997	Results demonstrated that synthesized niosomes had spherical-like, in the range of 60-80 nm with desirable cellular uptake.
37156313	2	72	theme	many	257:260	arg1	efforts					262:268	many efforts	257:268	many efforts	257:268	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	7	73	theme	sustained	1032:1040	arg1	release					1047:1053	a sustained drug release	1030:1053	a sustained drug release	1030:1053	Nio-PTX@GT-AL and Nio-PTX had a sustained drug release and were biodegradable.
37156313	2	74	theme	anti-cancer	287:297	arg1	drugs					299:303	anti-cancer drugs	287:303	anti-cancer drugs	287:303	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	1	75	theme	Breast	179:184	arg1	cancer					186:191	Breast cancer	179:191	Breast cancer	179:191	Breast cancer is one of the most lethal cancers, especially in women.
37156313	12	76	theme	effective	2032:2040	arg1	approach					2042:2049	an effective approach	2029:2049	an effective approach in designing nanocarriers for efficient drug delivery applications	2029:2116	The results of this study prove that employing 3D-printing and niosomal formulation is an effective approach in designing nanocarriers for efficient drug delivery applications.
37156313	11	77	theme	increased	1908:1916	arg1	apoptosis					1918:1926	increased apoptosis	1908:1926	increased apoptosis	1908:1926	Also, flow cytometry results declared that Nio-PTX@GT-AL reduced necrosis and increased apoptosis considerably.
37156313	4	78	theme	advanced	523:530	arg1	system					546:551	an advanced drug delivery system	520:551	an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	520:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	12	79	theme	3D-printing	1989:1999	arg1	formulation					2014:2024	3D-printing and niosomal formulation	1989:2024	3D-printing and niosomal formulation	1989:2024	The results of this study prove that employing 3D-printing and niosomal formulation is an effective approach in designing nanocarriers for efficient drug delivery applications.
37156313	0	80	theme	Large-scale	120:130	arg1	synthesis					132:140	Large-scale synthesis	120:140	Large-scale synthesis	120:140	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	7	81	theme	Nio-PTX	1000:1006	arg1	GT-AL					1008:1012	Nio-PTX@GT-AL	1000:1012	Nio-PTX@GT-AL	1000:1012	Nio-PTX@GT-AL and Nio-PTX had a sustained drug release and were biodegradable.
37156313	4	82	theme	delivery	537:544	arg1	system					546:551	an advanced drug delivery system	520:551	an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	520:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	12	83	theme	niosomal	2005:2012	arg1	formulation					2014:2024	3D-printing and niosomal formulation	1989:2024	3D-printing and niosomal formulation	1989:2024	The results of this study prove that employing 3D-printing and niosomal formulation is an effective approach in designing nanocarriers for efficient drug delivery applications.
37156313	3	84	theme	advanced	388:395	arg1	nanotechnology					434:447	nanotechnology	434:447	nanotechnology	434:447	Recently, advanced technologies such as 3D-printing and nanotechnology have created new horizons in cancer treatment.
37156313	3	84	theme	advanced	388:395	arg1	technologies					397:408	advanced technologies	388:408	advanced technologies such as 3D-printing and nanotechnology	388:447	Recently, advanced technologies such as 3D-printing and nanotechnology have created new horizons in cancer treatment.
37156313	3	84	theme	advanced	388:395	arg1	3D-printing					418:428	3D-printing	418:428	3D-printing	418:428	Recently, advanced technologies such as 3D-printing and nanotechnology have created new horizons in cancer treatment.
37156313	8	85	theme	anti-cancer	1326:1336	arg1	effects					1338:1344	the anti-cancer effects	1322:1344	the anti-cancer effects of control samples	1322:1363	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	0	86	theme	anti-cancer	155:165	arg1	evaluation					167:176	in-vitro anti-cancer evaluation	146:176	in-vitro anti-cancer evaluation	146:176	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	1	87	from	one	196:198	arg1	women					242:246	women	242:246	women	242:246	Breast cancer is one of the most lethal cancers, especially in women.
37156313	10	88	from	decrease	1751:1758	arg1	genes					1784:1788	metastasis-enhancing genes	1763:1788	metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9)	1763:1813	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	88	from	decrease	1751:1758	arg1	expression					1611:1620	expression	1611:1620	expression	1611:1620	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	88	from	decrease	1751:1758	arg1	MMP-9					1808:1812	MMP-9	1808:1812	MMP-9	1808:1812	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	88	from	decrease	1751:1758	arg1	activity					1626:1633	activity	1626:1633	activity	1626:1633	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	88	from	decrease	1751:1758	arg1	Bcl2					1791:1794	Bcl2	1791:1794	Bcl2	1791:1794	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	88	from	decrease	1751:1758	arg1	MMP-2					1797:1801	MMP-2	1797:1801	MMP-2	1797:1801	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	89	dep	metastasis	1708:1717	arg1	Bax					1720:1722	Bax	1720:1722	Bax	1720:1722	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	10	89	dep	metastasis	1708:1717	arg1	p53					1729:1731	p53	1729:1731	p53	1729:1731	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	5	90	theme	gene	761:764	arg1	expression					766:775	gene expression	761:775	gene expression	761:775	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	5	91	theme	control	817:823	arg1	samples					825:831	control samples	817:831	control samples	817:831	The morphology, drug release, degradation, cellular uptake, flow cytometry, cell cytotoxicity, migration, gene expression, and caspase activity of scaffolds, and control samples (Nio-PTX, and Free-PTX) were investigated.
37156313	8	92	theme	Cytotoxicity	1079:1090	arg1	studies					1092:1098	Cytotoxicity studies	1079:1098	Cytotoxicity studies	1079:1098	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	8	93	theme	samples	1357:1363	arg1	effects					1338:1344	the anti-cancer effects	1322:1344	the anti-cancer effects of control samples	1322:1363	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	11	94	theme	flow	1836:1839	arg1	cytometry					1841:1849	flow cytometry	1836:1849	flow cytometry results	1836:1857	Also, flow cytometry results declared that Nio-PTX@GT-AL reduced necrosis and increased apoptosis considerably.
37156313	0	95	theme	paclitaxel-loaded	34:50	arg1	niosomes					52:59	paclitaxel-loaded niosomes	34:59	paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite	34:117	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	4	96	theme	paclitaxel-loaded	611:627	arg1	niosomes					629:636	paclitaxel-loaded niosomes	611:636	paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	611:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	4	96	theme	paclitaxel-loaded	611:627	arg1	GT-AL					647:651	Nio-PTX@GT-AL	639:651	Nio-PTX@GT-AL	639:651	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	11	97	theme	@	1880:1880	arg1	GT-AL					1881:1885	Nio-PTX@GT-AL	1873:1885	Nio-PTX@GT-AL	1873:1885	Also, flow cytometry results declared that Nio-PTX@GT-AL reduced necrosis and increased apoptosis considerably.
37156313	0	98	theme	cross-linked	79:90	arg1	composite					109:117	cross-linked gelatin/alginate composite	79:117	cross-linked gelatin/alginate composite	79:117	3D-printing-assisted synthesis of paclitaxel-loaded niosomes functionalized by cross-linked gelatin/alginate composite: Large-scale synthesis and in-vitro anti-cancer evaluation.
37156313	8	99	theme	Nio-PTX	1127:1133	arg1	scaffold					1141:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	4	100	theme	3D-printed	562:571	arg1	scaffolds					590:598	3D-printed gelatin-alginate scaffolds	562:598	3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL)	562:652	In this work, we report an advanced drug delivery system based on 3D-printed gelatin-alginate scaffolds containing paclitaxel-loaded niosomes (Nio-PTX@GT-AL).
37156313	10	101	theme	nanocarrier	1512:1522	arg1	effect					1489:1494	The anticancer effect	1474:1494	The anticancer effect of the designed nanocarrier	1474:1522	The anticancer effect of the designed nanocarrier could be attributed to gene expression regulation, where a significant increase in the expression and activity of genes promoting apoptosis (CASP-3, CASP-8, and CASP-9) and inhibiting metastasis (Bax, and p53) and a remarkable decrease in metastasis-enhancing genes (Bcl2, MMP-2, and MMP-9) were observed.
37156313	2	102	theme	cancer	360:365	arg1	treatment					367:375	breast cancer treatment	353:375	breast cancer treatment	353:375	Despite many efforts, side effects of anti-cancer drugs and metastasis are still the main challenges in breast cancer treatment.
37156313	9	103	theme	migration	1369:1377	arg1	scratch-assay					1391:1403	scratch-assay	1391:1403	scratch-assay	1391:1403	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	9	103	theme	migration	1369:1377	arg1	evaluation					1379:1388	migration evaluation	1369:1388	migration evaluation (scratch-assay)	1369:1404	In migration evaluation (scratch-assay), approximately 70 % reduction of covered surface area was observed.
37156313	8	104	theme	GT-AL	1135:1139	arg1	scaffold					1141:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	the designed Nio-PTX@GT-AL scaffold	1114:1148	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	8	105	dep	cancer	1267:1272	arg1	cells					1274:1278	cells	1274:1278	cells (MCF-7)	1274:1286	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	8	105	dep	cancer	1267:1272	arg1	MCF-7					1281:1285	MCF-7	1281:1285	MCF-7	1281:1285	Cytotoxicity studies revealed that the designed Nio-PTX@GT-AL scaffold had <5 % cytotoxicity against non-tumorigenic breast cell line (MCF-10A) but showed 80 % cytotoxicity against breast cancer cells (MCF-7), which was considerably more than the anti-cancer effects of control samples.
37156313	12	106	theme	drug	2091:2094	arg1	delivery					2096:2103	efficient drug delivery	2081:2103	efficient drug delivery applications	2081:2116	The results of this study prove that employing 3D-printing and niosomal formulation is an effective approach in designing nanocarriers for efficient drug delivery applications.
37156313	3	107	theme	cancer	478:483	arg1	treatment					485:493	cancer treatment	478:493	cancer treatment	478:493	Recently, advanced technologies such as 3D-printing and nanotechnology have created new horizons in cancer treatment.
36347953	2	0	with	embedded	420:427	arg1	nanoparticles					441:453	silver nanoparticles	434:453	silver nanoparticles	434:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	4	1	theme	composite	772:780	arg1	alcohol					764:770	polyvinyl alcohol composite	754:780	polyvinyl alcohol composite prepared through solvent casting	754:813	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	1	2	theme	marine	177:182	arg1	food					184:187	marine food	177:187	marine food	177:187	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	1	3	theme	biodegradable	253:265	arg1	materials					277:285	biodegradable packaging materials	253:285	biodegradable packaging materials	253:285	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	6	4	theme	films	993:997	arg1	potency					978:984	The antimicrobial potency	960:984	The antimicrobial potency of the films	960:997	The antimicrobial potency of the films was evaluated using the disc diffusion antimicrobial assay against selected microorganisms.
36347953	2	5	theme	cellulose	367:375	arg1	nanocomposite					406:418	cellulose nanofibril-polyvinyl alcohol nanocomposite	367:418	cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	367:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	1	6	theme	packaging	267:275	arg1	materials					277:285	biodegradable packaging materials	253:285	biodegradable packaging materials	253:285	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	0	7	theme	alcohol	113:119	arg1	properties					89:98	the mechanical and antimicrobial properties	56:98	the mechanical and antimicrobial properties of polyvinyl alcohol	56:119	Cellulose nanofibrils and silver nanoparticles enhances the mechanical and antimicrobial properties of polyvinyl alcohol nanocomposite film.
36347953	1	8	from	food	184:187	arg1	findings					148:155	Recent findings	141:155	Recent findings of microplastics in marine food such as fish, crabs and shrimps	141:219	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	3	9	theme	NaOH/urea/water	601:615	arg1	solution					617:624	NaOH/urea/water solution	601:624	NaOH/urea/water solution	601:624	Microcrystalline cellulose was isolated from sugarcane bagasse via the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution.
36347953	0	10	theme	polyvinyl	103:111	arg1	alcohol					113:119	polyvinyl alcohol	103:119	polyvinyl alcohol	103:119	Cellulose nanofibrils and silver nanoparticles enhances the mechanical and antimicrobial properties of polyvinyl alcohol nanocomposite film.
36347953	1	11	from	microplastics	160:172	arg1	food					184:187	marine food	177:187	marine food	177:187	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	2	12	theme	nanofibril-polyvinyl	377:396	arg1	nanocomposite					406:418	cellulose nanofibril-polyvinyl alcohol nanocomposite	367:418	cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	367:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	4	13	from	element	743:749	arg1	alcohol					764:770	polyvinyl alcohol composite	754:780	polyvinyl alcohol composite prepared through solvent casting	754:813	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	4	14	theme	solvent	799:805	arg1	casting					807:813	solvent casting	799:813	solvent casting	799:813	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	5	15	theme	tensile	820:826	arg1	strength					828:835	tensile strength	820:835	tensile strength	820:835	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	3	16	theme	sugarcane	501:509	arg1	bagasse					511:517	sugarcane bagasse	501:517	sugarcane bagasse	501:517	Microcrystalline cellulose was isolated from sugarcane bagasse via the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution.
36347953	5	17	theme	water	876:880	arg1	permeability					888:899	water vapor permeability	876:899	water vapor permeability	876:899	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	6	18	theme	antimicrobial	964:976	arg1	potency					978:984	The antimicrobial potency	960:984	The antimicrobial potency of the films	960:997	The antimicrobial potency of the films was evaluated using the disc diffusion antimicrobial assay against selected microorganisms.
36347953	5	19	theme	optical	856:862	arg1	properties					864:873	optical properties	856:873	optical properties	856:873	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	1	20	from	findings	148:155	arg1	food					184:187	marine food	177:187	marine food	177:187	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	2	21	theme	silver	434:439	arg1	nanoparticles					441:453	silver nanoparticles	434:453	silver nanoparticles	434:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	5	22	theme	vapor	882:886	arg1	permeability					888:899	water vapor permeability	876:899	water vapor permeability	876:899	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	6	23	theme	antimicrobial	1038:1050	arg1	assay					1052:1056	the disc diffusion antimicrobial assay	1019:1056	the disc diffusion antimicrobial assay	1019:1056	The antimicrobial potency of the films was evaluated using the disc diffusion antimicrobial assay against selected microorganisms.
36347953	0	24	theme	Cellulose	0:8	arg1	nanofibrils					10:20	Cellulose nanofibrils	0:20	Cellulose nanofibrils	0:20	Cellulose nanofibrils and silver nanoparticles enhances the mechanical and antimicrobial properties of polyvinyl alcohol nanocomposite film.
36347953	6	25	theme	selected	1066:1073	arg1	microorganisms					1075:1088	selected microorganisms	1066:1088	selected microorganisms	1066:1088	The antimicrobial potency of the films was evaluated using the disc diffusion antimicrobial assay against selected microorganisms.
36347953	2	26	from	nanocomposite	406:418	arg1	material					353:360	a biodegradable packing material	329:360	a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	329:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	2	26	from	nanocomposite	406:418	arg1	development					314:324	the development	310:324	the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	310:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	2	27	theme	embedded	420:427	arg1	nanocomposite					406:418	cellulose nanofibril-polyvinyl alcohol nanocomposite	367:418	cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	367:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	0	28	theme	silver	26:31	arg1	nanoparticles					33:45	silver nanoparticles	26:45	silver nanoparticles	26:45	Cellulose nanofibrils and silver nanoparticles enhances the mechanical and antimicrobial properties of polyvinyl alcohol nanocomposite film.
36347953	3	29	attach	isolated	487:494	arg1	bagasse					511:517	sugarcane bagasse	501:517	sugarcane bagasse	501:517	Microcrystalline cellulose was isolated from sugarcane bagasse via the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution.
36347953	3	29	attach	isolated	487:494	arg2	cellulose					473:481	Microcrystalline cellulose	456:481	Microcrystalline cellulose	456:481	Microcrystalline cellulose was isolated from sugarcane bagasse via the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution.
36347953	1	30	theme	crabs	203:207	arg1	findings					148:155	Recent findings	141:155	Recent findings of microplastics in marine food such as fish, crabs and shrimps	141:219	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	4	31	used	used	721:724	arg2	element					743:749	a reinforcing element	729:749	a reinforcing element in polyvinyl alcohol composite prepared through solvent casting	729:813	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	4	31	used	used	721:724	arg2	nanofibrils					631:641	The nanofibrils	627:641	The nanofibrils	627:641	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	4	32	theme	polyvinyl	754:762	arg1	alcohol					764:770	polyvinyl alcohol composite	754:780	polyvinyl alcohol composite prepared through solvent casting	754:813	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	3	33	theme	cellulose	567:575	arg1	conversion					553:562	conversion	553:562	conversion of cellulose I to cellulose II using NaOH/urea/water solution	553:624	Microcrystalline cellulose was isolated from sugarcane bagasse via the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution.
36347953	2	34	theme	material	353:360	arg1	development					314:324	the development	310:324	the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	310:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	5	35	theme	prepared	924:931	arg1	films					933:937	the prepared films	920:937	the prepared films	920:937	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	4	36	theme	reinforcing	731:741	arg1	nanofibrils					631:641	The nanofibrils	627:641	The nanofibrils	627:641	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	4	36	theme	reinforcing	731:741	arg1	element					743:749	a reinforcing element	729:749	a reinforcing element in polyvinyl alcohol composite prepared through solvent casting	729:813	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	1	37	theme	shrimps	213:219	arg1	findings					148:155	Recent findings	141:155	Recent findings of microplastics in marine food such as fish, crabs and shrimps	141:219	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	6	38	theme	diffusion	1028:1036	arg1	assay					1052:1056	the disc diffusion antimicrobial assay	1019:1056	the disc diffusion antimicrobial assay	1019:1056	The antimicrobial potency of the films was evaluated using the disc diffusion antimicrobial assay against selected microorganisms.
36347953	1	39	from	shrimps	213:219	arg1	food					184:187	marine food	177:187	marine food	177:187	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	5	40	theme	films	933:937	arg1	permeability					888:899	water vapor permeability	876:899	water vapor permeability	876:899	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	5	40	theme	films	933:937	arg1	strength					828:835	tensile strength	820:835	tensile strength	820:835	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	5	40	theme	films	933:937	arg1	solubility					844:853	water solubility	838:853	water solubility	838:853	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	5	40	theme	films	933:937	arg1	properties					864:873	optical properties	856:873	optical properties	856:873	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	5	40	theme	films	933:937	arg1	wettability					905:915	wettability	905:915	wettability	905:915	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	0	41	theme	mechanical	60:69	arg1	properties					89:98	the mechanical and antimicrobial properties	56:98	the mechanical and antimicrobial properties of polyvinyl alcohol	56:119	Cellulose nanofibrils and silver nanoparticles enhances the mechanical and antimicrobial properties of polyvinyl alcohol nanocomposite film.
36347953	6	42	theme	disc	1023:1026	arg1	assay					1052:1056	the disc diffusion antimicrobial assay	1019:1056	the disc diffusion antimicrobial assay	1019:1056	The antimicrobial potency of the films was evaluated using the disc diffusion antimicrobial assay against selected microorganisms.
36347953	3	43	theme	Microcrystalline	456:471	arg1	cellulose					473:481	Microcrystalline cellulose	456:481	Microcrystalline cellulose	456:481	Microcrystalline cellulose was isolated from sugarcane bagasse via the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution.
36347953	1	44	from	crabs	203:207	arg1	food					184:187	marine food	177:187	marine food	177:187	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	1	45	theme	Recent	141:146	arg1	findings					148:155	Recent findings	141:155	Recent findings of microplastics in marine food such as fish, crabs and shrimps	141:219	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	2	46	theme	packing	345:351	arg1	material					353:360	a biodegradable packing material	329:360	a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	329:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	2	47	theme	biodegradable	331:343	arg1	material					353:360	a biodegradable packing material	329:360	a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	329:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	4	48	theme	2,2,6,6-Tetramethylpiperidin-1-yl	669:701	arg1	TEMPO					710:714	TEMPO	710:714	TEMPO	710:714	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	4	48	theme	2,2,6,6-Tetramethylpiperidin-1-yl	669:701	arg1	oxyl					704:707	(2,2,6,6-Tetramethylpiperidin-1-yl) oxyl	668:707	(2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO)	668:715	The nanofibrils were then isolated using (2,2,6,6-Tetramethylpiperidin-1-yl) oxyl (TEMPO) and used as a reinforcing element in polyvinyl alcohol composite prepared through solvent casting.
36347953	0	49	theme	antimicrobial	75:87	arg1	properties					89:98	the mechanical and antimicrobial properties	56:98	the mechanical and antimicrobial properties of polyvinyl alcohol	56:119	Cellulose nanofibrils and silver nanoparticles enhances the mechanical and antimicrobial properties of polyvinyl alcohol nanocomposite film.
36347953	0	50	dep	nanofibrils	10:20	arg1	enhances					47:54	enhances	47:54	enhances the mechanical and antimicrobial properties of polyvinyl alcohol	47:119	Cellulose nanofibrils and silver nanoparticles enhances the mechanical and antimicrobial properties of polyvinyl alcohol nanocomposite film.
36347953	3	51	theme	kraft	527:531	arg1	process					533:539	the kraft process	523:539	the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution	523:624	Microcrystalline cellulose was isolated from sugarcane bagasse via the kraft process followed by conversion of cellulose I to cellulose II using NaOH/urea/water solution.
36347953	1	52	theme	microplastics	160:172	arg1	findings					148:155	Recent findings	141:155	Recent findings of microplastics in marine food such as fish, crabs and shrimps	141:219	Recent findings of microplastics in marine food such as fish, crabs and shrimps necessitate the need to develop biodegradable packaging materials.
36347953	5	53	theme	water	838:842	arg1	solubility					844:853	water solubility	838:853	water solubility	838:853	The tensile strength, water solubility, optical properties, water vapor permeability and wettability of the prepared films were then evaluated.
36347953	2	54	theme	alcohol	398:404	arg1	nanocomposite					406:418	cellulose nanofibril-polyvinyl alcohol nanocomposite	367:418	cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	367:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
36347953	2	55	from	development	314:324	arg1	nanocomposite					406:418	cellulose nanofibril-polyvinyl alcohol nanocomposite	367:418	cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles	367:453	This study reports on the development of a biodegradable packing material from cellulose nanofibril-polyvinyl alcohol nanocomposite embedded with silver nanoparticles.
35435704	22	0	theme	evolutionary	2969:2980	arg1	trajectory					2982:2991	the evolutionary trajectory	2965:2991	the evolutionary trajectory of chronic infections	2965:3013	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	12	1	with	fusion	1562:1567	arg1	granules					1586:1593	neighboring granules	1574:1593	neighboring granules	1574:1593	We speculate that AlgP may contribute to spacing by tethering polyP granules to the chromosome, thereby inhibiting fusion with neighboring granules.
35435704	4	2	theme	critical	394:401	arg1	role					403:406	the critical role	390:406	the critical role of polyP in starvation fitness	390:437	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	21	3	theme	hot	2767:2769	arg1	AlgP					2757:2760	AlgP	2757:2760	AlgP	2757:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	3	theme	hot	2767:2769	arg1	domain					2747:2752	the C-terminal repeat domain	2725:2752	the C-terminal repeat domain of AlgP	2725:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	3	theme	hot	2767:2769	arg1	spot					2771:2774	a hot spot	2765:2774	a hot spot for genetic rearrangements	2765:2801	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	9	4	theme	sequence	1171:1178	arg1	composition					1180:1190	an overall sequence composition	1160:1190	an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1	1160:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	18	5	from	important	2369:2377	arg1	formation					2390:2398	biofilm formation	2382:2398	biofilm formation	2382:2398	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	5	6	from	starvation	707:716	arg1	pathogen					745:752	the opportunistic human pathogen	721:752	the opportunistic human pathogen Pseudomonas aeruginosa	721:775	We previously determined that granules become transiently evenly spaced on the cell's long axis during nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa.
35435704	22	7	theme	mutations	2952:2960	arg1	significance					2930:2941	the functional significance	2915:2941	the functional significance of these mutations in the evolutionary trajectory of chronic infections	2915:3013	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	4	8	from	role	403:406	arg1	fitness					431:437	starvation fitness	420:437	starvation fitness	420:437	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	13	9	theme	future	1752:1757	arg1	efforts					1759:1765	future efforts	1752:1765	future efforts	1752:1765	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	6	10	theme	granule-enrichment	799:816	arg1	protocol					818:825	a granule-enrichment protocol	797:825	a granule-enrichment protocol to screen for polyP granule-localizing proteins	797:873	Here, we developed a granule-enrichment protocol to screen for polyP granule-localizing proteins.
35435704	1	11	theme	universal	164:172	arg1	stress					174:179	stress	174:179	stress	174:179	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	1	11	theme	universal	164:172	arg1	Synthesis					111:119	Synthesis	111:119	Synthesis of polyphosphate (polyP)	111:144	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	8	12	theme	polyP	1013:1017	arg1	granules					1019:1026	polyP granules	1013:1026	polyP granules	1013:1026	We further discovered that AlgP is required for the even spacing of polyP granules.
35435704	13	13	theme	even	1722:1725	arg1	spacing					1727:1733	even spacing	1722:1733	even spacing	1722:1733	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	18	14	theme	chronic	2435:2441	arg1	infections					2443:2452	cystic fibrosis (CF) chronic infections	2414:2452	cystic fibrosis (CF) chronic infections	2414:2452	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	16	15	theme	spatial	2148:2154	arg1	specificity					2156:2166	spatial specificity	2148:2166	spatial specificity	2148:2166	However, polyP forms granule superstructures with spatial specificity.
35435704	20	16	theme	subcellular	2621:2631	arg1	organization					2633:2644	the subcellular organization	2617:2644	the subcellular organization of polyP, another polymer important for pathogenesis	2617:2697	We establish a distinct, previously unknown function for AlgP in modulating the subcellular organization of polyP, another polymer important for pathogenesis.
35435704	13	17	theme	functional	1782:1791	arg1	significance					1793:1804	the functional significance	1778:1804	the functional significance of granule organization on fitness	1778:1839	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	15	18	contain	has	2038:2040	arg1	lack					2002:2005	This simple polyanion's lack	1978:2005	This simple polyanion's lack of protein binding specificity	1978:2036	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	15	18	contain	has	2038:2040	arg2	validation					2050:2059	impeded validation	2042:2059	impeded validation of bona fide polyP-binding proteins	2042:2095	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	18	19	theme	biofilm	2382:2388	arg1	formation					2390:2398	biofilm formation	2382:2398	biofilm formation	2382:2398	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	13	20	from	significance	1793:1804	arg1	fitness					1833:1839	fitness	1833:1839	fitness	1833:1839	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	9	21	theme	eukaryotic	1226:1235	arg1	H1					1245:1246	eukaryotic histone H1	1226:1246	eukaryotic histone H1	1226:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	2	22	theme	many	221:224	arg1	bacteria					226:233	many bacteria	221:233	many bacteria	221:233	In many bacteria, polyP chains come together to form granular superstructures within cells.
35435704	5	23	dep	pathogen	745:752	arg1	aeruginosa					766:775	Pseudomonas aeruginosa	754:775	the opportunistic human pathogen Pseudomonas aeruginosa	721:775	We previously determined that granules become transiently evenly spaced on the cell's long axis during nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa.
35435704	18	24	theme	important	2369:2377	arg1	regulator					2314:2322	a regulator	2312:2322	a regulator of alginate	2312:2334	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	18	24	theme	important	2369:2377	arg1	polysaccharide					2354:2367	an extracellular polysaccharide	2337:2367	an extracellular polysaccharide important in biofilm formation	2337:2398	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	5	25	theme	long	681:684	arg1	axis					686:689	the cell's long axis	670:689	the cell's long axis	670:689	We previously determined that granules become transiently evenly spaced on the cell's long axis during nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa.
35435704	9	26	theme	H1	1245:1246	arg1	tail					1218:1221	the C-terminal tail	1203:1221	the C-terminal tail of eukaryotic histone H1	1203:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	1	27	theme	polyphosphate	124:136	arg1	stress					174:179	stress	174:179	stress	174:179	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	1	27	theme	polyphosphate	124:136	arg1	Synthesis					111:119	Synthesis	111:119	Synthesis of polyphosphate (polyP)	111:144	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	21	28	theme	CF	2703:2704	arg1	isolates					2715:2722	CF clinical isolates	2703:2722	CF clinical isolates	2703:2722	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	14	29	from	effects	1919:1925	arg1	physiology					1951:1960	bacterial starvation physiology	1930:1960	bacterial starvation physiology	1930:1960	IMPORTANCE The mechanisms underpinning polyP's pleiotropic effects on bacterial starvation physiology remain elusive.
35435704	12	30	theme	tethering	1499:1507	arg1	granules					1515:1522	tethering polyP granules	1499:1522	tethering polyP granules	1499:1522	We speculate that AlgP may contribute to spacing by tethering polyP granules to the chromosome, thereby inhibiting fusion with neighboring granules.
35435704	17	31	from	protein	2239:2245	arg1	aeruginosa					2262:2271	Pseudomonas aeruginosa	2250:2271	Pseudomonas aeruginosa	2250:2271	Our granule enrichment protocol identified a polyP granule-associated protein in Pseudomonas aeruginosa, AlgP.
35435704	10	32	theme	Granule	1249:1255	arg1	size					1257:1260	Granule size	1249:1260	Granule size	1249:1260	Granule size, number, and spacing are significantly perturbed in the absence of AlgP, or when AlgP is truncated to remove the C-terminus.
35435704	0	33	theme	Granules	75:82	arg1	Spacing					50:56	Even Spacing	45:56	Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa	45:108	The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
35435704	1	34	theme	ancient	152:158	arg1	stress					174:179	stress	174:179	stress	174:179	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	1	34	theme	ancient	152:158	arg1	Synthesis					111:119	Synthesis	111:119	Synthesis of polyphosphate (polyP)	111:144	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	3	35	theme	granule	348:354	arg1	organization					368:379	polyP granule subcellular organization	342:379	polyP granule subcellular organization	342:379	Some species appear to regulate polyP granule subcellular organization.
35435704	21	36	theme	repeat	2740:2745	arg1	AlgP					2757:2760	AlgP	2757:2760	AlgP	2757:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	36	theme	repeat	2740:2745	arg1	domain					2747:2752	the C-terminal repeat domain	2725:2752	the C-terminal repeat domain of AlgP	2725:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	36	theme	repeat	2740:2745	arg1	spot					2771:2774	a hot spot	2765:2774	a hot spot for genetic rearrangements	2765:2801	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	9	37	theme	amino	1070:1074	arg1	domain					1092:1097	a 154 amino acid C-terminal domain	1064:1097	a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat	1064:1152	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	1	38	from	stress	174:179	arg1	bacteria					208:215	bacteria	208:215	bacteria	208:215	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	4	39	theme	structures	465:474	arg1	composition					444:454	the composition	440:454	the composition of these structures	440:474	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	4	39	theme	structures	465:474	arg1	s					487:487	mechanism(s)	477:488	mechanism(s) underpinning their organization	477:520	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	4	39	theme	structures	465:474	arg1	significance					538:549	functional significance	527:549	functional significance of such organization	527:570	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	9	40	theme	C-terminal	1081:1090	arg1	domain					1092:1097	a 154 amino acid C-terminal domain	1064:1097	a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat	1064:1152	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	20	41	theme	important	2672:2680	arg1	polyP					2649:2653	polyP	2649:2653	polyP	2649:2653	We establish a distinct, previously unknown function for AlgP in modulating the subcellular organization of polyP, another polymer important for pathogenesis.
35435704	20	41	theme	important	2672:2680	arg1	polymer					2664:2670	another polymer	2656:2670	another polymer important for pathogenesis	2656:2697	We establish a distinct, previously unknown function for AlgP in modulating the subcellular organization of polyP, another polymer important for pathogenesis.
35435704	4	42	theme	mechanism	477:485	arg1	s					487:487	mechanism(s)	477:488	mechanism(s) underpinning their organization	477:520	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	16	43	theme	granule	2119:2125	arg1	superstructures					2127:2141	granule superstructures	2119:2141	granule superstructures	2119:2141	However, polyP forms granule superstructures with spatial specificity.
35435704	17	44	theme	granule-associated	2220:2237	arg1	AlgP					2274:2277	AlgP	2274:2277	AlgP	2274:2277	Our granule enrichment protocol identified a polyP granule-associated protein in Pseudomonas aeruginosa, AlgP.
35435704	17	44	theme	granule-associated	2220:2237	arg1	protein					2239:2245	a polyP granule-associated protein	2212:2245	a polyP granule-associated protein in Pseudomonas aeruginosa	2212:2271	Our granule enrichment protocol identified a polyP granule-associated protein in Pseudomonas aeruginosa, AlgP.
35435704	11	45	theme	ΔalgP	1391:1395	arg1	mutants					1410:1416	The ΔalgP and algPΔCTD mutants	1387:1416	The ΔalgP and algPΔCTD mutants	1387:1416	The ΔalgP and algPΔCTD mutants have fewer, larger granules.
35435704	19	46	theme	putative	2462:2469	arg1	role					2471:2474	AlgP's putative role	2455:2474	AlgP's putative role in alginate biosynthesis	2455:2499	AlgP's putative role in alginate biosynthesis has recently been called into question.
35435704	15	47	theme	impeded	2042:2048	arg1	validation					2050:2059	impeded validation	2042:2059	impeded validation of bona fide polyP-binding proteins	2042:2095	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	0	48	theme	H1-Like	12:18	arg1	AlgP					28:31	The Histone H1-Like Protein AlgP	0:31	The Histone H1-Like Protein AlgP	0:31	The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
35435704	18	49	theme	fibrosis	2421:2428	arg1	infections					2443:2452	cystic fibrosis (CF) chronic infections	2414:2452	cystic fibrosis (CF) chronic infections	2414:2452	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	13	50	theme	granule	1809:1815	arg1	organization					1817:1828	granule organization	1809:1828	granule organization	1809:1828	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	4	51	theme	such	554:557	arg1	organization					559:570	such organization	554:570	such organization	554:570	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	9	52	theme	"	1116:1116	arg1	repeats					1118:1124	"KPAA" repeats	1111:1124	"KPAA" repeats	1111:1124	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	18	53	theme	alginate	2327:2334	arg1	regulator					2314:2322	a regulator	2312:2322	a regulator of alginate	2312:2334	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	18	53	theme	alginate	2327:2334	arg1	polysaccharide					2354:2367	an extracellular polysaccharide	2337:2367	an extracellular polysaccharide important in biofilm formation	2337:2398	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	18	53	theme	alginate	2327:2334	arg1	AlgP					2280:2283	AlgP	2280:2283	AlgP	2280:2283	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	15	54	theme	simple	1983:1988	arg1	lack					2002:2005	This simple polyanion's lack	1978:2005	This simple polyanion's lack of protein binding specificity	1978:2036	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	0	55	theme	Even	45:48	arg1	Spacing					50:56	Even Spacing	45:56	Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa	45:108	The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
35435704	14	56	theme	starvation	1940:1949	arg1	physiology					1951:1960	bacterial starvation physiology	1930:1960	bacterial starvation physiology	1930:1960	IMPORTANCE The mechanisms underpinning polyP's pleiotropic effects on bacterial starvation physiology remain elusive.
35435704	9	57	theme	similar	1192:1198	arg1	composition					1180:1190	an overall sequence composition	1160:1190	an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1	1160:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	6	58	theme	granule-localizing	847:864	arg1	proteins					866:873	polyP granule-localizing proteins	841:873	polyP granule-localizing proteins	841:873	Here, we developed a granule-enrichment protocol to screen for polyP granule-localizing proteins.
35435704	21	59	theme	genetic	2780:2786	arg1	rearrangements					2788:2801	genetic rearrangements	2780:2801	genetic rearrangements	2780:2801	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	17	60	theme	granule	2173:2179	arg1	protocol					2192:2199	Our granule enrichment protocol	2169:2199	Our granule enrichment protocol	2169:2199	Our granule enrichment protocol identified a polyP granule-associated protein in Pseudomonas aeruginosa, AlgP.
35435704	9	61	theme	repeat	1147:1152	arg1	variants					1130:1137	variants	1130:1137	variants of this repeat	1130:1152	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	9	61	theme	repeat	1147:1152	arg1	repeats					1118:1124	"KPAA" repeats	1111:1124	"KPAA" repeats	1111:1124	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	15	62	theme	specificity	2026:2036	arg1	lack					2002:2005	This simple polyanion's lack	1978:2005	This simple polyanion's lack of protein binding specificity	1978:2036	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	7	63	theme	polyP	929:933	arg1	granules					935:942	polyP granules	929:942	polyP granules	929:942	We identified AlgP as a protein that associates with polyP granules.
35435704	11	64	contain	have	1418:1421	arg2	granules					1437:1444	fewer, larger granules	1423:1444	fewer, larger granules	1423:1444	The ΔalgP and algPΔCTD mutants have fewer, larger granules.
35435704	11	64	contain	have	1418:1421	arg1	mutants					1410:1416	The ΔalgP and algPΔCTD mutants	1387:1416	The ΔalgP and algPΔCTD mutants	1387:1416	The ΔalgP and algPΔCTD mutants have fewer, larger granules.
35435704	15	65	theme	proteins	2088:2095	arg1	validation					2050:2059	impeded validation	2042:2059	impeded validation of bona fide polyP-binding proteins	2042:2095	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	21	66	theme	AlgP	2757:2760	arg1	AlgP					2757:2760	AlgP	2757:2760	AlgP	2757:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	66	theme	AlgP	2757:2760	arg1	domain					2747:2752	the C-terminal repeat domain	2725:2752	the C-terminal repeat domain of AlgP	2725:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	66	theme	AlgP	2757:2760	arg1	spot					2771:2774	a hot spot	2765:2774	a hot spot for genetic rearrangements	2765:2801	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	10	67	theme	AlgP	1329:1332	arg1	absence					1318:1324	the absence	1314:1324	the absence of AlgP	1314:1332	Granule size, number, and spacing are significantly perturbed in the absence of AlgP, or when AlgP is truncated to remove the C-terminus.
35435704	8	68	theme	even	997:1000	arg1	spacing					1002:1008	the even spacing	993:1008	the even spacing of polyP granules	993:1026	We further discovered that AlgP is required for the even spacing of polyP granules.
35435704	9	69	theme	overall	1163:1169	arg1	composition					1180:1190	an overall sequence composition	1160:1190	an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1	1160:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	2	70	theme	granular	271:278	arg1	superstructures					280:294	granular superstructures	271:294	granular superstructures	271:294	In many bacteria, polyP chains come together to form granular superstructures within cells.
35435704	4	71	theme	polyP	411:415	arg1	role					403:406	the critical role	390:406	the critical role of polyP in starvation fitness	390:437	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	8	72	theme	granules	1019:1026	arg1	spacing					1002:1008	the even spacing	993:1008	the even spacing of polyP granules	993:1026	We further discovered that AlgP is required for the even spacing of polyP granules.
35435704	18	73	theme	cystic	2414:2419	arg1	CF					2431:2432	CF	2431:2432	CF	2431:2432	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	18	73	theme	cystic	2414:2419	arg1	fibrosis					2421:2428	cystic fibrosis	2414:2428	cystic fibrosis (CF) chronic infections	2414:2452	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	22	74	from	significance	2930:2941	arg1	trajectory					2982:2991	the evolutionary trajectory	2965:2991	the evolutionary trajectory of chronic infections	2965:3013	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	20	75	theme	polyP	2649:2653	arg1	organization					2633:2644	the subcellular organization	2617:2644	the subcellular organization of polyP, another polymer important for pathogenesis	2617:2697	We establish a distinct, previously unknown function for AlgP in modulating the subcellular organization of polyP, another polymer important for pathogenesis.
35435704	5	76	theme	nitrogen	698:705	arg1	starvation					707:716	nitrogen starvation	698:716	nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa	698:775	We previously determined that granules become transiently evenly spaced on the cell's long axis during nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa.
35435704	9	77	theme	C-terminal	1207:1216	arg1	tail					1218:1221	the C-terminal tail	1203:1221	the C-terminal tail of eukaryotic histone H1	1203:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	21	78	from	spot	2771:2774	arg1	isolates					2715:2722	CF clinical isolates	2703:2722	CF clinical isolates	2703:2722	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	18	79	from	formation	2390:2398	arg1	important					2369:2377	important	2369:2377	important	2369:2377	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	1	80	theme	starvation	185:194	arg1	response					196:203	starvation response	185:203	starvation response	185:203	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	12	81	theme	neighboring	1574:1584	arg1	granules					1586:1593	neighboring granules	1574:1593	neighboring granules	1574:1593	We speculate that AlgP may contribute to spacing by tethering polyP granules to the chromosome, thereby inhibiting fusion with neighboring granules.
35435704	19	82	theme	alginate	2479:2486	arg1	biosynthesis					2488:2499	alginate biosynthesis	2479:2499	alginate biosynthesis	2479:2499	AlgP's putative role in alginate biosynthesis has recently been called into question.
35435704	13	83	theme	granule	1698:1704	arg1	biogenesis					1706:1715	granule biogenesis	1698:1715	granule biogenesis	1698:1715	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	9	84	theme	histone	1237:1243	arg1	H1					1245:1246	eukaryotic histone H1	1226:1246	eukaryotic histone H1	1226:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	4	85	theme	starvation	420:429	arg1	fitness					431:437	starvation fitness	420:437	starvation fitness	420:437	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	1	86	from	response	196:203	arg1	bacteria					208:215	bacteria	208:215	bacteria	208:215	Synthesis of polyphosphate (polyP) is an ancient and universal stress and starvation response in bacteria.
35435704	12	87	theme	polyP	1509:1513	arg1	granules					1515:1522	tethering polyP granules	1499:1522	tethering polyP granules	1499:1522	We speculate that AlgP may contribute to spacing by tethering polyP granules to the chromosome, thereby inhibiting fusion with neighboring granules.
35435704	13	88	theme	organization	1817:1828	arg1	significance					1793:1804	the functional significance	1778:1804	the functional significance of granule organization on fitness	1778:1839	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	9	89	theme	DNA-binding	1039:1049	arg1	protein					1051:1057	a DNA-binding protein	1037:1057	a DNA-binding protein	1037:1057	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	9	89	theme	DNA-binding	1039:1049	arg1	AlgP					1029:1032	AlgP	1029:1032	AlgP	1029:1032	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	0	90	theme	Polyphosphate	61:73	arg1	Granules					75:82	Polyphosphate Granules	61:82	Polyphosphate Granules	61:82	The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
35435704	2	91	theme	polyP	236:240	arg1	chains					242:247	polyP chains	236:247	polyP chains	236:247	In many bacteria, polyP chains come together to form granular superstructures within cells.
35435704	11	92	dep	fewer	1423:1427	arg1	larger					1430:1435	larger	1430:1435	larger	1430:1435	The ΔalgP and algPΔCTD mutants have fewer, larger granules.
35435704	5	93	theme	human	739:743	arg1	pathogen					745:752	the opportunistic human pathogen	721:752	the opportunistic human pathogen Pseudomonas aeruginosa	721:775	We previously determined that granules become transiently evenly spaced on the cell's long axis during nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa.
35435704	13	94	theme	first	1666:1670	arg1	time					1672:1675	the first time	1662:1675	the first time	1662:1675	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	21	95	theme	C-terminal	2729:2738	arg1	AlgP					2757:2760	AlgP	2757:2760	AlgP	2757:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	95	theme	C-terminal	2729:2738	arg1	domain					2747:2752	the C-terminal repeat domain	2725:2752	the C-terminal repeat domain of AlgP	2725:2760	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	21	95	theme	C-terminal	2729:2738	arg1	spot					2771:2774	a hot spot	2765:2774	a hot spot for genetic rearrangements	2765:2801	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	5	96	theme	opportunistic	725:737	arg1	pathogen					745:752	the opportunistic human pathogen	721:752	the opportunistic human pathogen Pseudomonas aeruginosa	721:775	We previously determined that granules become transiently evenly spaced on the cell's long axis during nitrogen starvation in the opportunistic human pathogen Pseudomonas aeruginosa.
35435704	15	97	theme	bona	2064:2067	arg1	proteins					2088:2095	bona fide polyP-binding proteins	2064:2095	bona fide polyP-binding proteins	2064:2095	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	3	98	theme	subcellular	356:366	arg1	organization					368:379	polyP granule subcellular organization	342:379	polyP granule subcellular organization	342:379	Some species appear to regulate polyP granule subcellular organization.
35435704	0	99	from	Spacing	50:56	arg1	aeruginosa					99:108	Pseudomonas aeruginosa	87:108	Pseudomonas aeruginosa	87:108	The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
35435704	20	100	dep	distinct	2556:2563	arg1	unknown					2577:2583	unknown	2577:2583	unknown	2577:2583	We establish a distinct, previously unknown function for AlgP in modulating the subcellular organization of polyP, another polymer important for pathogenesis.
35435704	9	101	theme	acid	1076:1079	arg1	domain					1092:1097	a 154 amino acid C-terminal domain	1064:1097	a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat	1064:1152	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	9	102	with	protein	1051:1057	arg1	domain					1092:1097	a 154 amino acid C-terminal domain	1064:1097	a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat	1064:1152	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	9	102	with	protein	1051:1057	arg1	composition					1180:1190	an overall sequence composition	1160:1190	an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1	1160:1246	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	22	103	theme	chronic	2996:3002	arg1	infections					3004:3013	chronic infections	2996:3013	chronic infections	2996:3013	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	3	104	theme	polyP	342:346	arg1	organization					368:379	polyP granule subcellular organization	342:379	polyP granule subcellular organization	342:379	Some species appear to regulate polyP granule subcellular organization.
35435704	21	105	theme	clinical	2706:2713	arg1	isolates					2715:2722	CF clinical isolates	2703:2722	CF clinical isolates	2703:2722	In CF clinical isolates, the C-terminal repeat domain of AlgP is a hot spot for genetic rearrangements.
35435704	17	106	theme	polyP	2214:2218	arg1	AlgP					2274:2277	AlgP	2274:2277	AlgP	2274:2277	Our granule enrichment protocol identified a polyP granule-associated protein in Pseudomonas aeruginosa, AlgP.
35435704	17	106	theme	polyP	2214:2218	arg1	protein					2239:2245	a polyP granule-associated protein	2212:2245	a polyP granule-associated protein in Pseudomonas aeruginosa	2212:2271	Our granule enrichment protocol identified a polyP granule-associated protein in Pseudomonas aeruginosa, AlgP.
35435704	0	107	theme	Histone	4:10	arg1	AlgP					28:31	The Histone H1-Like Protein AlgP	0:31	The Histone H1-Like Protein AlgP	0:31	The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
35435704	19	108	from	role	2471:2474	arg1	biosynthesis					2488:2499	alginate biosynthesis	2479:2499	alginate biosynthesis	2479:2499	AlgP's putative role in alginate biosynthesis has recently been called into question.
35435704	22	109	theme	granule	2860:2866	arg1	organization					2868:2879	granule organization	2860:2879	granule organization	2860:2879	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	11	110	theme	fewer	1423:1427	arg1	granules					1437:1444	fewer, larger granules	1423:1444	fewer, larger granules	1423:1444	The ΔalgP and algPΔCTD mutants have fewer, larger granules.
35435704	14	111	dep	IMPORTANCE	1860:1869	arg1	mechanisms					1875:1884	The mechanisms	1871:1884	IMPORTANCE The mechanisms underpinning polyP's pleiotropic effects on bacterial starvation physiology	1860:1960	IMPORTANCE The mechanisms underpinning polyP's pleiotropic effects on bacterial starvation physiology remain elusive.
35435704	0	112	theme	Protein	20:26	arg1	AlgP					28:31	The Histone H1-Like Protein AlgP	0:31	The Histone H1-Like Protein AlgP	0:31	The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa.
35435704	15	113	theme	binding	2018:2024	arg1	specificity					2026:2036	protein binding specificity	2010:2036	protein binding specificity	2010:2036	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	18	114	theme	extracellular	2340:2352	arg1	regulator					2314:2322	a regulator	2312:2322	a regulator of alginate	2312:2334	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	18	114	theme	extracellular	2340:2352	arg1	polysaccharide					2354:2367	an extracellular polysaccharide	2337:2367	an extracellular polysaccharide important in biofilm formation	2337:2398	AlgP was originally reported as a regulator of alginate, an extracellular polysaccharide important in biofilm formation, including in cystic fibrosis (CF) chronic infections.
35435704	4	115	theme	organization	559:570	arg1	composition					444:454	the composition	440:454	the composition of these structures	440:474	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	4	115	theme	organization	559:570	arg1	s					487:487	mechanism(s)	477:488	mechanism(s) underpinning their organization	477:520	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	4	115	theme	organization	559:570	arg1	significance					538:549	functional significance	527:549	functional significance of such organization	527:570	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	15	116	theme	protein	2010:2016	arg1	specificity					2026:2036	protein binding specificity	2010:2036	protein binding specificity	2010:2036	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	9	117	theme	KPAA	1112:1115	arg1	repeats					1118:1124	"KPAA" repeats	1111:1124	"KPAA" repeats	1111:1124	AlgP is a DNA-binding protein with a 154 amino acid C-terminal domain enriched in "KPAA" repeats and variants of this repeat, with an overall sequence composition similar to the C-terminal tail of eukaryotic histone H1.
35435704	11	118	theme	algPΔCTD	1401:1408	arg1	mutants					1410:1416	The ΔalgP and algPΔCTD mutants	1387:1416	The ΔalgP and algPΔCTD mutants	1387:1416	The ΔalgP and algPΔCTD mutants have fewer, larger granules.
35435704	13	119	theme	granule	1632:1638	arg1	spacing					1640:1646	granule spacing	1632:1646	granule spacing	1632:1646	Our discovery that AlgP facilitates granule spacing allows us for the first time to directly uncouple granule biogenesis from even spacing, and will inform future efforts to explore the functional significance of granule organization on fitness during starvation.
35435704	4	120	theme	functional	527:536	arg1	significance					538:549	functional significance	527:549	functional significance of such organization	527:570	Despite the critical role of polyP in starvation fitness, the composition of these structures, mechanism(s) underpinning their organization, and functional significance of such organization are poorly understood.
35435704	14	121	theme	pleiotropic	1907:1917	arg1	effects					1919:1925	polyP's pleiotropic effects	1899:1925	polyP's pleiotropic effects on bacterial starvation physiology	1899:1960	IMPORTANCE The mechanisms underpinning polyP's pleiotropic effects on bacterial starvation physiology remain elusive.
35435704	22	122	theme	functional	2919:2928	arg1	significance					2930:2941	the functional significance	2915:2941	the functional significance of these mutations in the evolutionary trajectory of chronic infections	2915:3013	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	14	123	theme	bacterial	1930:1938	arg1	physiology					1951:1960	bacterial starvation physiology	1930:1960	bacterial starvation physiology	1930:1960	IMPORTANCE The mechanisms underpinning polyP's pleiotropic effects on bacterial starvation physiology remain elusive.
35435704	15	124	dep	bona	2064:2067	arg1	fide					2069:2072	fide	2069:2072	fide	2069:2072	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	22	125	theme	infections	3004:3013	arg1	trajectory					2982:2991	the evolutionary trajectory	2965:2991	the evolutionary trajectory of chronic infections	2965:3013	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	6	126	theme	polyP	841:845	arg1	proteins					866:873	polyP granule-localizing proteins	841:873	polyP granule-localizing proteins	841:873	Here, we developed a granule-enrichment protocol to screen for polyP granule-localizing proteins.
35435704	15	127	theme	polyP-binding	2074:2086	arg1	proteins					2088:2095	bona fide polyP-binding proteins	2064:2095	bona fide polyP-binding proteins	2064:2095	This simple polyanion's lack of protein binding specificity has impeded validation of bona fide polyP-binding proteins.
35435704	22	128	theme	AlgP	2839:2842	arg1	C-terminus					2825:2834	the C-terminus	2821:2834	the C-terminus of AlgP	2821:2842	Our finding that the C-terminus of AlgP is required for granule organization lays the groundwork for exploring the functional significance of these mutations in the evolutionary trajectory of chronic infections.
35435704	17	129	theme	enrichment	2181:2190	arg1	protocol					2192:2199	Our granule enrichment protocol	2169:2199	Our granule enrichment protocol	2169:2199	Our granule enrichment protocol identified a polyP granule-associated protein in Pseudomonas aeruginosa, AlgP.
35435704	20	130	theme	distinct	2556:2563	arg1	function					2585:2592	a distinct, previously unknown function	2554:2592	a distinct, previously unknown function for AlgP	2554:2601	We establish a distinct, previously unknown function for AlgP in modulating the subcellular organization of polyP, another polymer important for pathogenesis.
35269946	0	0	theme	Human	82:86	arg1	Intakes					109:115	Human Milk Oligosaccharide Intakes	82:115	Human Milk Oligosaccharide Intakes	82:115	Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes.
35269946	3	1	theme	full-length	638:648	arg1	gene					675:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	1	2	theme	microbiomes	162:172	arg1	development					127:137	Temporal development	118:137	Temporal development of maternal and infant microbiomes during early life	118:190	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	6	3	theme	Beta	942:945	arg1	diversity					947:955	Beta diversity	942:955	Beta diversity	942:955	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	9	4	theme	infant	1517:1522	arg1	development					1535:1545	infant microbiota development	1517:1545	infant microbiota development	1517:1545	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	6	5	theme	HM	1020:1021	arg1	samples					1023:1029	infant faecal, maternal faecal and HM samples	985:1029	samples	1023:1029	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	0	6	theme	Oligosaccharide	93:107	arg1	Intakes					109:115	Human Milk Oligosaccharide Intakes	82:115	Human Milk Oligosaccharide Intakes	82:115	Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes.
35269946	8	7	theme	direct	1317:1322	arg1	unknown					1364:1370	unknown	1364:1370	unknown	1364:1370	The direct clinical relevance of this, however, is unknown.
35269946	8	7	theme	direct	1317:1322	arg1	relevance					1333:1341	The direct clinical relevance	1313:1341	The direct clinical relevance of this	1313:1349	The direct clinical relevance of this, however, is unknown.
35269946	9	8	theme	microbiota	1524:1533	arg1	development					1535:1545	infant microbiota development	1517:1545	infant microbiota development	1517:1545	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	6	9	theme	p	1134:1134	arg1	points					1122:1127	all other time points	1107:1127	all other time points (all p < 0.02)	1107:1142	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	6	9	theme	p	1134:1134	arg1	<					1136:1136	all p < 0.02	1130:1141	all p < 0.02	1130:1141	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	3	10	theme	Maternal	535:542	arg1	samples					555:561	Maternal and infant samples	535:561	Maternal and infant samples (n = 10)	535:570	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	3	10	theme	Maternal	535:542	arg1	n					564:564	n = 10	564:569	n = 10	564:569	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	0	11	theme	Milk	88:91	arg1	Intakes					109:115	Human Milk Oligosaccharide Intakes	82:115	Human Milk Oligosaccharide Intakes	82:115	Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes.
35269946	3	12	theme	RNA	664:666	arg1	gene					675:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	5	13	theme	variation	911:919	arg1	degree					873:878	a large degree	865:878	a large degree of intra- and inter-individual variation in all sample types	865:939	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	2	14	theme	faecal	304:309	arg1	samples					360:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	5	15	theme	large	867:871	arg1	degree					873:878	a large degree	865:878	a large degree of intra- and inter-individual variation in all sample types	865:939	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	1	16	theme	Temporal	118:125	arg1	development					127:137	Temporal development	118:137	Temporal development of maternal and infant microbiomes during early life	118:190	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	6	17	theme	infant	985:990	arg1	faecal					1009:1014	infant faecal, maternal faecal and HM samples	985:1029	faecal	1009:1014	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	2	18	theme	maternal	295:302	arg1	samples					360:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	7	19	theme	HMO	1214:1216	arg1	intakes					1218:1224	HMO intakes	1214:1224	HMO intakes	1214:1224	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	1	20	theme	early	181:185	arg1	life					187:190	early life	181:190	early life	181:190	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	2	21	theme	oral	499:502	arg1	profiles					525:532	infant oral and faecal bacterial profiles	492:532	infant oral and faecal bacterial profiles	492:532	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	3	22	theme	16S	650:652	arg1	gene					675:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	2	23	theme	human	447:451	arg1	intakes					480:486	human milk oligosaccharide (HMO) intakes	447:486	human milk oligosaccharide (HMO) intakes	447:486	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	5	24	theme	sample	811:816	arg1	type					818:821	each sample type	806:821	each sample type	806:821	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	0	25	theme	Breastfed	12:20	arg1	Microbiota					29:38	Exclusively Breastfed Infant Microbiota	0:38	Exclusively Breastfed Infant Microbiota	0:38	Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes.
35269946	3	26	theme	ribosomal	654:662	arg1	gene					675:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	7	27	with	associations	1246:1257	arg1	taxa					1264:1267	taxa	1264:1267	taxa observed in infant oral and faecal samples	1264:1310	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	5	28	theme	intra-	883:888	arg1	degree					873:878	a large degree	865:878	a large degree of intra- and inter-individual variation in all sample types	865:939	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	6	29	theme	maternal	1000:1007	arg1	faecal					1009:1014	infant faecal, maternal faecal and HM samples	985:1029	faecal	1009:1014	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	6	30	theme	oral	1052:1055	arg1	microbiota					1057:1066	the infant oral microbiota	1041:1066	the infant oral microbiota at day 2−5	1041:1077	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	1	31	theme	short-	200:205	arg1	health					228:233	short- and long-term infant health	200:233	short- and long-term infant health	200:233	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	6	32	theme	time	1117:1120	arg1	points					1122:1127	all other time points	1107:1127	all other time points (all p < 0.02)	1107:1142	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	6	32	theme	time	1117:1120	arg1	<					1136:1136	all p < 0.02	1130:1141	all p < 0.02	1130:1141	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	9	33	from	impact	1454:1459	arg1	growth					1477:1482	infant growth	1470:1482	infant growth	1470:1482	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	2	34	theme	milk	318:321	arg1	samples					360:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	5	35	theme	inter-individual	894:909	arg1	variation					911:919	inter-individual variation	894:919	inter-individual variation	894:919	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	0	36	theme	Infant	22:27	arg1	Microbiota					29:38	Exclusively Breastfed Infant Microbiota	0:38	Exclusively Breastfed Infant Microbiota	0:38	Exclusively Breastfed Infant Microbiota Develops over Time and Is Associated with Human Milk Oligosaccharide Intakes.
35269946	3	37	theme	rRNA	669:672	arg1	gene					675:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	the full-length 16S ribosomal RNA (rRNA) gene	634:678	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	2	38	theme	human	312:316	arg1	milk					318:321	human milk	312:321	human milk (HM)	312:326	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	2	38	theme	human	312:316	arg1	HM					324:325	HM	324:325	HM	324:325	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	1	39	theme	long-term	211:219	arg1	health					228:233	short- and long-term infant health	200:233	short- and long-term infant health	200:233	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	2	40	theme	HMO	475:477	arg1	intakes					480:486	human milk oligosaccharide (HMO) intakes	447:486	human milk oligosaccharide (HMO) intakes	447:486	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	9	41	theme	future	1385:1390	arg1	studies					1392:1398	future studies	1385:1398	future studies	1385:1398	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	9	42	theme	HM	1464:1465	arg1	impact					1454:1459	the impact	1450:1459	the impact of HM on infant growth	1450:1482	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	7	43	theme	HMO	1145:1147	arg1	concentrations					1149:1162	HMO concentrations	1145:1162	HMO concentrations	1145:1162	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	9	44	theme	infant	1470:1475	arg1	growth					1477:1482	infant growth	1470:1482	infant growth	1470:1482	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	6	45	from	day	1071:1073	arg1	microbiota					1057:1066	the infant oral microbiota	1041:1066	the infant oral microbiota at day 2−5	1041:1077	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	1	46	theme	infant	221:226	arg1	health					228:233	short- and long-term infant health	200:233	short- and long-term infant health	200:233	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	2	47	theme	exclusive	379:387	arg1	period					403:408	the exclusive breastfeeding period	375:408	the exclusive breastfeeding period	375:408	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	9	48	contain	have	1495:1498	arg2	implications					1500:1511	implications	1500:1511	implications for infant microbiota development	1500:1545	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	9	48	contain	have	1495:1498	arg1	it					1488:1489	it	1488:1489	it	1488:1489	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	7	49	theme	faecal	1297:1302	arg1	samples					1304:1310	infant oral and faecal samples	1281:1310	samples	1304:1310	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	4	50	theme	liquid	749:754	arg1	chromatography					756:769	high-performance liquid chromatography	732:769	high-performance liquid chromatography	732:769	Nineteen HMOs were quantitated using high-performance liquid chromatography.
35269946	2	51	theme	breastfeeding	389:401	arg1	period					403:408	the exclusive breastfeeding period	375:408	the exclusive breastfeeding period	375:408	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	2	52	theme	oligosaccharide	458:472	arg1	intakes					480:486	human milk oligosaccharide (HMO) intakes	447:486	human milk oligosaccharide (HMO) intakes	447:486	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	2	53	theme	bacterial	515:523	arg1	profiles					525:532	infant oral and faecal bacterial profiles	492:532	infant oral and faecal bacterial profiles	492:532	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	3	54	theme	=	566:566	arg1	samples					555:561	Maternal and infant samples	535:561	Maternal and infant samples (n = 10)	535:570	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	3	54	theme	=	566:566	arg1	n					564:564	n = 10	564:569	n = 10	564:569	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	2	55	theme	oral	336:339	arg1	samples					360:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	2	56	theme	milk	453:456	arg1	intakes					480:486	human milk oligosaccharide (HMO) intakes	447:486	human milk oligosaccharide (HMO) intakes	447:486	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	9	57	theme	HMOs	1430:1433	arg1	intakes					1419:1425	intakes	1419:1425	intakes of HMOs	1419:1433	Regardless, future studies should account for intakes of HMOs when modelling the impact of HM on infant growth, as it may have implications for infant microbiota development.
35269946	1	58	theme	infant	155:160	arg1	microbiomes					162:172	maternal and infant microbiomes	142:172	maternal and infant microbiomes	142:172	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	6	59	theme	infant	1045:1050	arg1	microbiota					1057:1066	the infant oral microbiota	1041:1066	the infant oral microbiota at day 2−5	1041:1077	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	8	60	theme	this	1346:1349	arg1	unknown					1364:1370	unknown	1364:1370	unknown	1364:1370	The direct clinical relevance of this, however, is unknown.
35269946	8	60	theme	this	1346:1349	arg1	relevance					1333:1341	The direct clinical relevance	1313:1341	The direct clinical relevance of this	1313:1349	The direct clinical relevance of this, however, is unknown.
35269946	6	61	theme	other	1111:1115	arg1	points					1122:1127	all other time points	1107:1127	all other time points (all p < 0.02)	1107:1142	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	6	61	theme	other	1111:1115	arg1	<					1136:1136	all p < 0.02	1130:1141	all p < 0.02	1130:1141	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	3	62	theme	days	614:617	arg1	postpartum					619:628	2−5, 30, 60, 90 and 120 days postpartum	590:628	2−5, 30, 60, 90 and 120 days postpartum	590:628	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	5	63	theme	sample	928:933	arg1	types					935:939	all sample types	924:939	all sample types	924:939	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	8	64	theme	clinical	1324:1331	arg1	unknown					1364:1370	unknown	1364:1370	unknown	1364:1370	The direct clinical relevance of this, however, is unknown.
35269946	8	64	theme	clinical	1324:1331	arg1	relevance					1333:1341	The direct clinical relevance	1313:1341	The direct clinical relevance of this	1313:1349	The direct clinical relevance of this, however, is unknown.
35269946	3	65	theme	infant	548:553	arg1	samples					555:561	Maternal and infant samples	535:561	Maternal and infant samples (n = 10)	535:570	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	3	65	theme	infant	548:553	arg1	n					564:564	n = 10	564:569	n = 10	564:569	Maternal and infant samples (n = 10) were collected at 2−5, 30, 60, 90 and 120 days postpartum and the full-length 16S ribosomal RNA (rRNA) gene was sequenced.
35269946	7	66	located	observed	1269:1276	arg1	oral					1288:1291	oral	1288:1291	oral	1288:1291	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	7	66	located	observed	1269:1276	arg1	samples					1304:1310	infant oral and faecal samples	1281:1310	samples	1304:1310	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	7	66	located	observed	1269:1276	arg2	taxa					1264:1267	taxa	1264:1267	taxa observed in infant oral and faecal samples	1264:1310	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	2	67	theme	bacterial	269:277	arg1	dynamics					279:286	bacterial dynamics	269:286	bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples	269:366	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	1	68	theme	maternal	142:149	arg1	microbiomes					162:172	maternal and infant microbiomes	142:172	maternal and infant microbiomes	142:172	Temporal development of maternal and infant microbiomes during early life impacts short- and long-term infant health.
35269946	5	69	from	degree	873:878	arg1	types					935:939	all sample types	924:939	all sample types	924:939	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	2	70	theme	infant	492:497	arg1	profiles					525:532	infant oral and faecal bacterial profiles	492:532	infant oral and faecal bacterial profiles	492:532	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	4	71	theme	high-performance	732:747	arg1	chromatography					756:769	high-performance liquid chromatography	732:769	high-performance liquid chromatography	732:769	Nineteen HMOs were quantitated using high-performance liquid chromatography.
35269946	6	72	theme	faecal	992:997	arg1	faecal					1009:1014	infant faecal, maternal faecal and HM samples	985:1029	faecal	1009:1014	Beta diversity was stable over time within infant faecal, maternal faecal and HM samples, however, the infant oral microbiota at day 2−5 significantly differed from all other time points (all p < 0.02).
35269946	2	73	theme	faecal	353:358	arg1	samples					360:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	7	74	theme	differential	1233:1244	arg1	associations					1246:1257	differential associations	1233:1257	differential associations with taxa observed in infant oral and faecal samples	1233:1310	HMO concentrations and intakes significantly differed over time, and HMO intakes showed differential associations with taxa observed in infant oral and faecal samples.
35269946	5	75	theme	Bacterial	772:780	arg1	profiles					782:789	Bacterial profiles	772:789	Bacterial profiles	772:789	Bacterial profiles were unique to each sample type and changed significantly over time, with a large degree of intra- and inter-individual variation in all sample types.
35269946	2	76	theme	infant	346:351	arg1	samples					360:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	maternal faecal, human milk (HM), infant oral, and infant faecal samples	295:366	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
35269946	2	77	theme	faecal	508:513	arg1	profiles					525:532	infant oral and faecal bacterial profiles	492:532	infant oral and faecal bacterial profiles	492:532	This study aimed to characterize bacterial dynamics within maternal faecal, human milk (HM), infant oral, and infant faecal samples during the exclusive breastfeeding period and to document associations between human milk oligosaccharide (HMO) intakes and infant oral and faecal bacterial profiles.
37227128	7	0	theme	antioxidant	1151:1161	arg1	codelivery					1121:1130	The codelivery	1117:1130	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines	1117:1249	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	0	1	dep	Treatment	144:152	arg1	Stability					14:22	Combined Core Stability	0:22	Combined Core Stability	0:22	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	0	1	dep	Treatment	144:152	arg1	Degradability					28:40	Degradability	28:40	Degradability	28:40	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	8	2	theme	senescence-accelerated	1426:1447	arg1	SAMP8					1464:1468	senescence-accelerated mouse prone 8 (SAMP8)	1426:1469	senescence-accelerated mouse prone 8 (SAMP8)	1426:1469	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	0	3	theme	Bottlebrush	86:96	arg1	Copolymer					98:106	Amorphous PDLLA-Dextran Bottlebrush Copolymer	62:106	Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease	62:130	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	3	4	theme	PLLA	682:685	arg1	polymer					687:693	the crystalline PLLA polymer	666:693	the crystalline PLLA polymer	666:693	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	2	5	from	effects	364:370	arg1	tissues					398:404	normal tissues	391:404	normal tissues	391:404	However, core cross-linking leads to biodegradability deficiency and brings inevitable side effects of nanomedicine on normal tissues.
37227128	7	6	theme	hydrophilic	1139:1149	arg1	antioxidant					1151:1161	the hydrophilic antioxidant	1135:1161	the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA)	1135:1219	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	8	7	theme	mouse	1449:1453	arg1	SAMP8					1464:1468	senescence-accelerated mouse prone 8 (SAMP8)	1426:1469	senescence-accelerated mouse prone 8 (SAMP8)	1426:1469	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	4	8	theme	influence	781:789	arg1	roles					791:795	important influence roles	771:795	important influence roles	771:795	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	8	9	theme	cognitive	1403:1411	arg1	abilities					1413:1421	the cognitive abilities	1399:1421	the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8)	1399:1469	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	2	10	theme	biodegradability	309:324	arg1	deficiency					326:335	biodegradability deficiency	309:335	biodegradability deficiency	309:335	However, core cross-linking leads to biodegradability deficiency and brings inevitable side effects of nanomedicine on normal tissues.
37227128	6	11	dep	stability	1071:1079	arg1	the					1057:1059	the	1057:1059	the	1057:1059	Here, the amorphous bottlebrush PDLLA was verified to play a beneficial role in the structure stability and degradability of nanomedicines.
37227128	7	12	theme	optimum	1229:1235	arg1	nanomedicines					1237:1249	the optimum nanomedicines	1225:1249	the optimum nanomedicines	1225:1249	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	4	13	theme	PDLLA	749:753	arg1	density					706:712	graft density	700:712	graft density	700:712	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	4	13	theme	PDLLA	749:753	arg1	length					729:734	side chain length	718:734	side chain length	718:734	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	2	14	theme	normal	391:396	arg1	tissues					398:404	normal tissues	391:404	normal tissues	391:404	However, core cross-linking leads to biodegradability deficiency and brings inevitable side effects of nanomedicine on normal tissues.
37227128	4	15	dep	density	706:712	arg1	The					696:698	The	696:698	The	696:698	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	3	16	theme	lactic	483:488	arg1	acid					490:493	lactic acid	483:493	lactic acid	483:493	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	6	17	theme	nanomedicines	1102:1114	arg1	degradability					1085:1097	degradability	1085:1097	degradability	1085:1097	Here, the amorphous bottlebrush PDLLA was verified to play a beneficial role in the structure stability and degradability of nanomedicines.
37227128	6	17	theme	nanomedicines	1102:1114	arg1	stability					1071:1079	structure stability	1061:1079	structure stability	1061:1079	Here, the amorphous bottlebrush PDLLA was verified to play a beneficial role in the structure stability and degradability of nanomedicines.
37227128	4	18	theme	important	771:779	arg1	roles					791:795	important influence roles	771:795	important influence roles	771:795	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	1	19	theme	infinite	192:199	arg1	dilution					201:208	infinite dilution	192:208	infinite dilution	192:208	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37227128	3	20	theme	bottleneck	433:442	arg1	problem					444:450	this bottleneck problem	428:450	this bottleneck problem	428:450	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	3	21	dep	poly	474:477	arg1	l					481:481	l	481:481	l	481:481	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	3	21	dep	poly	474:477	arg1	d					479:479	d	479:479	d	479:479	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	7	22	theme	cell	1288:1291	arg1	damage					1293:1298	the SH-SY5Y cell damage	1276:1298	the SH-SY5Y cell damage caused by H2O2	1276:1313	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	6	23	theme	beneficial	1038:1047	arg1	role					1049:1052	a beneficial role	1036:1052	a beneficial role	1036:1052	Here, the amorphous bottlebrush PDLLA was verified to play a beneficial role in the structure stability and degradability of nanomedicines.
37227128	1	24	theme	dilution	201:208	arg1	challenges					178:187	the challenges	174:187	the challenges of infinite dilution, shear force, biological protein, or electrolyte competition	174:269	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37227128	0	25	theme	Core	9:12	arg1	Stability					14:22	Combined Core Stability	0:22	Combined Core Stability	0:22	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	7	26	theme	gallic	1204:1209	arg1	GA					1217:1218	GA	1217:1218	GA	1217:1218	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	7	26	theme	gallic	1204:1209	arg1	acid					1211:1214	gallic acid	1204:1214	gallic acid (GA)	1204:1219	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	7	27	theme	acid	1211:1214	arg1	antioxidant					1151:1161	the hydrophilic antioxidant	1135:1161	the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA)	1135:1219	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	2	28	theme	inevitable	348:357	arg1	effects					364:370	inevitable side effects	348:370	inevitable side effects of nanomedicine on normal tissues	348:404	However, core cross-linking leads to biodegradability deficiency and brings inevitable side effects of nanomedicine on normal tissues.
37227128	7	29	theme	vitamin	1184:1190	arg1	VC					1195:1196	VC	1195:1196	VC	1195:1196	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	7	29	theme	vitamin	1184:1190	arg1	C					1192:1192	vitamin C	1184:1192	vitamin C (VC)	1184:1197	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	0	30	theme	Combined	0:7	arg1	Stability					14:22	Combined Core Stability	0:22	Combined Core Stability	0:22	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	6	31	theme	bottlebrush	997:1007	arg1	PDLLA					1009:1013	the amorphous bottlebrush PDLLA	983:1013	the amorphous bottlebrush PDLLA	983:1013	Here, the amorphous bottlebrush PDLLA was verified to play a beneficial role in the structure stability and degradability of nanomedicines.
37227128	3	32	theme	nanoparticles	558:570	arg1	stability					545:553	the core stability	536:553	the core stability of nanoparticles	536:570	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	8	33	theme	combination	1329:1339	arg1	treatment					1341:1349	The CA/VC/GA combination treatment	1316:1349	The CA/VC/GA combination treatment	1316:1349	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	1	34	theme	shear	211:215	arg1	force					217:221	shear force	211:221	shear force	211:221	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37227128	6	35	theme	amorphous	987:995	arg1	PDLLA					1009:1013	the amorphous bottlebrush PDLLA	983:1013	the amorphous bottlebrush PDLLA	983:1013	Here, the amorphous bottlebrush PDLLA was verified to play a beneficial role in the structure stability and degradability of nanomedicines.
37227128	3	36	theme	core	540:543	arg1	stability					545:553	the core stability	536:553	the core stability of nanoparticles	536:570	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	1	37	theme	force	217:221	arg1	challenges					178:187	the challenges	174:187	the challenges of infinite dilution, shear force, biological protein, or electrolyte competition	174:269	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37227128	4	38	theme	chain	723:727	arg1	length					729:734	side chain length	718:734	side chain length	718:734	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	0	39	theme	Combination	132:142	arg1	Treatment					144:152	Combination Treatment	132:152	Combination Treatment	132:152	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	3	40	theme	fast	635:638	arg1	property					652:659	fast degradation property	635:659	fast degradation property	635:659	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	3	41	dep	-dextran	502:509	arg1	acid					490:493	lactic acid	483:493	lactic acid	483:493	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	6	42	theme	structure	1061:1069	arg1	stability					1071:1079	structure stability	1061:1079	structure stability	1061:1079	Here, the amorphous bottlebrush PDLLA was verified to play a beneficial role in the structure stability and degradability of nanomedicines.
37227128	5	43	theme	large	932:936	arg1	vesicles					947:954	large compound vesicles	932:954	large compound vesicles after self-assembly	932:974	This effort produces structure-abundant particles, including micelles, vesicles, and large compound vesicles after self-assembly.
37227128	3	44	theme	degradation	640:650	arg1	property					652:659	fast degradation property	635:659	fast degradation property	635:659	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	3	45	dep	we	453:454	arg1	turn					456:459	turn	456:459	turn	456:459	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	1	46	theme	biological	224:233	arg1	protein					235:241	biological protein	224:241	biological protein	224:241	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37227128	5	47	theme	structure-abundant	868:885	arg1	vesicles					918:925	vesicles	918:925	vesicles	918:925	This effort produces structure-abundant particles, including micelles, vesicles, and large compound vesicles after self-assembly.
37227128	5	47	theme	structure-abundant	868:885	arg1	particles					887:895	structure-abundant particles	868:895	structure-abundant particles	868:895	This effort produces structure-abundant particles, including micelles, vesicles, and large compound vesicles after self-assembly.
37227128	5	47	theme	structure-abundant	868:885	arg1	micelles					908:915	micelles	908:915	micelles	908:915	This effort produces structure-abundant particles, including micelles, vesicles, and large compound vesicles after self-assembly.
37227128	5	47	theme	structure-abundant	868:885	arg1	vesicles					947:954	large compound vesicles	932:954	large compound vesicles after self-assembly	932:974	This effort produces structure-abundant particles, including micelles, vesicles, and large compound vesicles after self-assembly.
37227128	3	48	theme	amorphous	581:589	arg1	structure					591:599	the amorphous structure	577:599	the amorphous structure	577:599	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	1	49	theme	protein	235:241	arg1	challenges					178:187	the challenges	174:187	the challenges of infinite dilution, shear force, biological protein, or electrolyte competition	174:269	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37227128	7	50	theme	citric	1166:1171	arg1	CA					1179:1180	CA	1179:1180	CA	1179:1180	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	7	50	theme	citric	1166:1171	arg1	acid					1173:1176	citric acid	1166:1176	citric acid (CA)	1166:1181	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	8	51	theme	prone	1455:1459	arg1	SAMP8					1464:1468	senescence-accelerated mouse prone 8 (SAMP8)	1426:1469	senescence-accelerated mouse prone 8 (SAMP8)	1426:1469	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	4	52	theme	nanoparticles	832:844	arg1	architecture					816:827	the architecture	812:827	the architecture of nanoparticles	812:844	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	2	53	theme	nanomedicine	375:386	arg1	effects					364:370	inevitable side effects	348:370	inevitable side effects of nanomedicine on normal tissues	348:404	However, core cross-linking leads to biodegradability deficiency and brings inevitable side effects of nanomedicine on normal tissues.
37227128	0	54	theme	Nanomedicine	45:56	arg1	Stability					14:22	Combined Core Stability	0:22	Combined Core Stability	0:22	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	0	54	theme	Nanomedicine	45:56	arg1	Degradability					28:40	Degradability	28:40	Degradability	28:40	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	8	55	theme	SAMP8	1464:1468	arg1	abilities					1413:1421	the cognitive abilities	1399:1421	the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8)	1399:1469	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	4	56	theme	graft	700:704	arg1	density					706:712	graft density	700:712	graft density	700:712	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	7	57	theme	acid	1173:1176	arg1	antioxidant					1151:1161	the hydrophilic antioxidant	1135:1161	the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA)	1135:1219	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	3	58	theme	crystalline	670:680	arg1	polymer					687:693	the crystalline PLLA polymer	666:693	the crystalline PLLA polymer	666:693	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	2	59	theme	core	281:284	arg1	cross-linking					286:298	core cross-linking	281:298	core cross-linking	281:298	However, core cross-linking leads to biodegradability deficiency and brings inevitable side effects of nanomedicine on normal tissues.
37227128	4	60	theme	side	718:721	arg1	length					729:734	side chain length	718:734	side chain length	718:734	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	4	61	theme	amorphous	739:747	arg1	PDLLA					749:753	amorphous PDLLA	739:753	amorphous PDLLA	739:753	The graft density and side chain length of amorphous PDLLA together played important influence roles in controlling the architecture of nanoparticles.
37227128	8	62	theme	CA/VC/GA	1320:1327	arg1	treatment					1341:1349	The CA/VC/GA combination treatment	1316:1349	The CA/VC/GA combination treatment	1316:1349	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	3	63	theme	property	652:659	arg1	advantage					622:630	an additional advantage	608:630	an additional advantage of fast degradation property over the crystalline PLLA polymer	608:693	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	3	64	theme	additional	611:620	arg1	advantage					622:630	an additional advantage	608:630	an additional advantage of fast degradation property over the crystalline PLLA polymer	608:693	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	2	65	theme	side	359:362	arg1	effects					364:370	inevitable side effects	348:370	inevitable side effects of nanomedicine on normal tissues	348:404	However, core cross-linking leads to biodegradability deficiency and brings inevitable side effects of nanomedicine on normal tissues.
37227128	5	66	theme	compound	938:945	arg1	vesicles					947:954	large compound vesicles	932:954	large compound vesicles after self-assembly	932:974	This effort produces structure-abundant particles, including micelles, vesicles, and large compound vesicles after self-assembly.
37227128	0	67	theme	PDLLA-Dextran	72:84	arg1	Copolymer					98:106	Amorphous PDLLA-Dextran Bottlebrush Copolymer	62:106	Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease	62:130	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	8	68	theme	neuronal	1364:1371	arg1	function					1373:1380	the neuronal function	1360:1380	the neuronal function	1360:1380	The CA/VC/GA combination treatment repaired the neuronal function efficiently, and the cognitive abilities of senescence-accelerated mouse prone 8 (SAMP8) recovered.
37227128	7	69	theme	SH-SY5Y	1280:1286	arg1	damage					1293:1298	the SH-SY5Y cell damage	1276:1298	the SH-SY5Y cell damage caused by H2O2	1276:1313	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	3	70	theme	amorphous	464:472	arg1	poly					474:477	amorphous poly	464:477	amorphous poly(d,l)lactic acid (PDLLA)-dextran	464:509	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	1	71	theme	electrolyte	247:257	arg1	competition					259:269	electrolyte competition	247:269	electrolyte competition	247:269	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37227128	0	72	theme	Amorphous	62:70	arg1	Copolymer					98:106	Amorphous PDLLA-Dextran Bottlebrush Copolymer	62:106	Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease	62:130	Combined Core Stability and Degradability of Nanomedicine via Amorphous PDLLA-Dextran Bottlebrush Copolymer for Alzheimer's Disease Combination Treatment.
37227128	3	73	theme	-dextran	502:509	arg1	poly					474:477	amorphous poly	464:477	amorphous poly(d,l)lactic acid (PDLLA)-dextran	464:509	In order to overcome this bottleneck problem, we turn to amorphous poly(d,l)lactic acid (PDLLA)-dextran bottlebrush to emphasize the core stability of nanoparticles, and the amorphous structure offers an additional advantage of fast degradation property over the crystalline PLLA polymer.
37227128	7	74	theme	C	1192:1192	arg1	antioxidant					1151:1161	the hydrophilic antioxidant	1135:1161	the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA)	1135:1219	The codelivery of the hydrophilic antioxidant of citric acid (CA), vitamin C (VC), and gallic acid (GA) via the optimum nanomedicines could effectively repair the SH-SY5Y cell damage caused by H2O2.
37227128	1	75	theme	competition	259:269	arg1	challenges					178:187	the challenges	174:187	the challenges of infinite dilution, shear force, biological protein, or electrolyte competition	174:269	Nanomedicine faces the challenges of infinite dilution, shear force, biological protein, or electrolyte competition.
37108742	2	0	theme	polyvinyl	427:435	arg1	BTCP-AE-FMs					470:480	BTCP-AE-FMs	470:480	BTCP-AE-FMs	470:480	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	0	theme	polyvinyl	427:435	arg1	meshes					462:467	polyvinyl alcohol/chitosan fibrous meshes	427:467	polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration	427:503	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	4	1	from	viability	711:719	arg1	BTCP-AE-FM					754:763	the BTCP-AE-FM	750:763	the BTCP-AE-FM	750:763	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	3	2	theme	147	591:593	arg1	±					595:595	a 147 ±	589:595	a 147 ± 50 nm fibrous structure	589:619	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	9	3	theme	hybrid	1456:1461	arg1	mesh					1491:1494	hybrid nanospun scaffold composite mesh	1456:1494	hybrid nanospun scaffold composite mesh	1456:1494	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	3	theme	hybrid	1456:1461	arg1	bone					1524:1527	a new experimental bone substitute bioactive material	1505:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	4	theme	scaffold	1472:1479	arg1	mesh					1491:1494	hybrid nanospun scaffold composite mesh	1456:1494	hybrid nanospun scaffold composite mesh	1456:1494	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	4	theme	scaffold	1472:1479	arg1	bone					1524:1527	a new experimental bone substitute bioactive material	1505:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	7	5	theme	bone	1182:1185	arg1	formations					1187:1196	New bone formations	1178:1196	New bone formations	1178:1196	New bone formations were confirmed by histological analysis.
37108742	3	6	theme	contact	643:649	arg1	angles					651:656	the contact angles	639:656	the contact angles	639:656	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	3	6	theme	contact	643:649	arg1	1.7°					670:673	64.1 ± 1.7°	663:673	64.1 ± 1.7°	663:673	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	5	7	theme	in	898:899	arg1	experiments					906:916	in vivo experiments	898:916	in vivo experiments	898:916	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	5	7	theme	in	898:899	arg1	defects					864:870	Critical-size calvarial defects	840:870	Critical-size calvarial defects in rats	840:878	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	5	8	theme	meshes	950:955	arg1	influence					937:945	the influence	933:945	the influence of meshes on bone regeneration	933:976	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	5	9	theme	bone	960:963	arg1	regeneration					965:976	bone regeneration	960:976	bone regeneration	960:976	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	1	10	theme	drug	239:242	arg1	delivery					244:251	nanofiber-based drug delivery	223:251	nanofiber-based drug delivery	223:251	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	6	11	theme	PET	979:981	arg1	imaging					983:989	PET imaging	979:989	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs)	979:1049	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	8	12	theme	mesh	1288:1291	arg1	morphology					1270:1279	the morphology	1266:1279	the morphology of the mesh because of cross-linking	1266:1316	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	3	13	theme	±	668:668	arg1	angles					651:656	the contact angles	639:656	the contact angles	639:656	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	3	13	theme	±	668:668	arg1	1.7°					670:673	64.1 ± 1.7°	663:673	64.1 ± 1.7°	663:673	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	9	14	theme	new	1507:1509	arg1	mesh					1491:1494	hybrid nanospun scaffold composite mesh	1456:1494	hybrid nanospun scaffold composite mesh	1456:1494	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	14	theme	new	1507:1509	arg1	bone					1524:1527	a new experimental bone substitute bioactive material	1505:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	4	15	theme	stem	736:739	arg1	cells					741:745	dental pulp stem cells	724:745	dental pulp stem cells	724:745	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	8	16	from	change	1256:1261	arg1	morphology					1270:1279	the morphology	1266:1279	the morphology of the mesh because of cross-linking	1266:1316	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	9	17	theme	bioactive	1540:1548	arg1	material					1550:1557	bioactive material	1540:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	5	18	theme	Critical-size	840:852	arg1	defects					864:870	Critical-size calvarial defects	840:870	Critical-size calvarial defects in rats	840:878	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	5	18	theme	Critical-size	840:852	arg1	experiments					906:916	in vivo experiments	898:916	in vivo experiments	898:916	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	4	19	theme	dental	724:729	arg1	cells					741:745	dental pulp stem cells	724:745	dental pulp stem cells	724:745	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	1	20	from	use	184:186	arg1	scaffolds					275:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	1	20	from	use	184:186	arg1	applications					202:213	biomedical applications	191:213	biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds	191:283	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	9	21	theme	medical	1569:1575	arg1	practice					1577:1584	future medical practice	1562:1584	future medical practice	1562:1584	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	6	22	theme	uptake	1030:1035	arg1	SUVs					1045:1048	SUVs	1045:1048	SUVs	1045:1048	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	6	22	theme	uptake	1030:1035	arg1	values					1037:1042	18F-sodium fluoride standardized uptake values	997:1042	18F-sodium fluoride standardized uptake values (SUVs)	997:1049	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	8	23	theme	fibrous	1357:1363	arg1	structure					1373:1381	its fibrous, porous structure	1353:1381	structure	1373:1381	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	7	24	theme	histological	1216:1227	arg1	analysis					1229:1236	histological analysis	1216:1236	histological analysis	1216:1236	New bone formations were confirmed by histological analysis.
37108742	6	25	theme	fluoride	1008:1015	arg1	SUVs					1045:1048	SUVs	1045:1048	SUVs	1045:1048	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	6	25	theme	fluoride	1008:1015	arg1	values					1037:1042	18F-sodium fluoride standardized uptake values	997:1042	18F-sodium fluoride standardized uptake values (SUVs)	997:1049	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	8	26	theme	porous	1366:1371	arg1	structure					1373:1381	its fibrous, porous structure	1353:1381	structure	1373:1381	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	6	27	theme	fibrous	1105:1111	arg1	FMs					1121:1123	FMs	1121:1123	FMs	1121:1123	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	6	27	theme	fibrous	1105:1111	arg1	meshes					1113:1118	polyvinyl alcohol/chitosan fibrous meshes	1078:1118	polyvinyl alcohol/chitosan fibrous meshes (FMs)	1078:1124	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	1	28	theme	potential	163:171	arg1	Electrospinning					113:127	Electrospinning	113:127	Electrospinning	113:127	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	1	28	theme	potential	163:171	arg1	method					173:178	a potential method	161:178	a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds	161:283	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	0	29	theme	β-Tricalcium	0:11	arg1	Aerogel					32:38	β-Tricalcium Phosphate-Modified Aerogel	0:38	β-Tricalcium Phosphate-Modified Aerogel	0:38	β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration.
37108742	2	30	dep	in	524:525	arg1	vivo					527:530	vivo	527:530	vivo	527:530	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	3	31	dep	±	595:595	arg1	structure					611:619	50 nm fibrous structure	597:619	a 147 ± 50 nm fibrous structure	589:619	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	6	32	theme	polyvinyl	1078:1086	arg1	FMs					1121:1123	FMs	1121:1123	FMs	1121:1123	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	6	32	theme	polyvinyl	1078:1086	arg1	meshes					1113:1118	polyvinyl alcohol/chitosan fibrous meshes	1078:1118	polyvinyl alcohol/chitosan fibrous meshes (FMs)	1078:1124	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	2	33	theme	in	511:512	arg1	conditions					532:541	in vitro and in vivo conditions	511:541	in vitro and in vivo conditions	511:541	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	7	34	theme	New	1178:1180	arg1	formations					1187:1196	New bone formations	1178:1196	New bone formations	1178:1196	New bone formations were confirmed by histological analysis.
37108742	0	35	theme	PVA/Chitosan	51:62	arg1	Scaffolds					80:88	PVA/Chitosan Hybrid Nanospun Scaffolds	51:88	PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration	51:110	β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration.
37108742	2	36	dep	in	511:512	arg1	vitro					514:518	vitro	514:518	vitro	514:518	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	0	37	theme	Nanospun	71:78	arg1	Scaffolds					80:88	PVA/Chitosan Hybrid Nanospun Scaffolds	51:88	PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration	51:110	β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration.
37108742	5	38	dep	in	898:899	arg1	vivo					901:904	vivo	901:904	vivo	901:904	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	3	39	theme	physicochemical	553:567	arg1	properties					569:578	The mesh physicochemical properties	544:578	The mesh physicochemical properties	544:578	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	1	40	theme	tissue	256:261	arg1	engineering					263:273	tissue engineering	256:273	tissue engineering	256:273	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	4	41	theme	scanning	810:817	arg1	microscope					828:837	a scanning electron microscope	808:837	a scanning electron microscope	808:837	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	3	42	theme	50	597:598	arg1	nm					600:601	nm	600:601	nm	600:601	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	6	43	with	±	1138:1138	arg1	BTCP-AE-FMs					1150:1160	BTCP-AE-FMs	1150:1160	BTCP-AE-FMs	1150:1160	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	2	44	theme	alcohol/chitosan	437:452	arg1	BTCP-AE-FMs					470:480	BTCP-AE-FMs	470:480	BTCP-AE-FMs	470:480	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	44	theme	alcohol/chitosan	437:452	arg1	meshes					462:467	polyvinyl alcohol/chitosan fibrous meshes	427:467	polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration	427:503	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	45	theme	electrospinning	329:343	arg1	preparation					345:355	the electrospinning preparation	325:355	the electrospinning preparation	325:355	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	3	46	theme	fibrous	603:609	arg1	structure					611:619	50 nm fibrous structure	597:619	a 147 ± 50 nm fibrous structure	589:619	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	1	47	theme	biomedical	191:200	arg1	scaffolds					275:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	1	47	theme	biomedical	191:200	arg1	applications					202:213	biomedical applications	191:213	biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds	191:283	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	8	48	theme	slight	1249:1254	arg1	change					1256:1261	a slight change	1247:1261	a slight change in the morphology of the mesh because of cross-linking	1247:1316	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	9	49	theme	composite	1481:1489	arg1	mesh					1491:1494	hybrid nanospun scaffold composite mesh	1456:1494	hybrid nanospun scaffold composite mesh	1456:1494	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	49	theme	composite	1481:1489	arg1	bone					1524:1527	a new experimental bone substitute bioactive material	1505:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	6	50	theme	18F-sodium	997:1006	arg1	SUVs					1045:1048	SUVs	1045:1048	SUVs	1045:1048	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	6	50	theme	18F-sodium	997:1006	arg1	values					1037:1042	18F-sodium fluoride standardized uptake values	997:1042	18F-sodium fluoride standardized uptake values (SUVs)	997:1049	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	4	51	theme	cells	741:745	arg1	viability					711:719	The viability	707:719	The viability of dental pulp stem cells on the BTCP-AE-FM	707:763	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	3	52	theme	aqueous	625:631	arg1	media					633:637	aqueous media	625:637	aqueous media	625:637	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	5	53	theme	calvarial	854:862	arg1	defects					864:870	Critical-size calvarial defects	840:870	Critical-size calvarial defects in rats	840:878	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	5	53	theme	calvarial	854:862	arg1	experiments					906:916	in vivo experiments	898:916	in vivo experiments	898:916	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	1	54	theme	delivery	244:251	arg1	scaffolds					275:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	9	55	theme	nanospun	1463:1470	arg1	mesh					1491:1494	hybrid nanospun scaffold composite mesh	1456:1494	hybrid nanospun scaffold composite mesh	1456:1494	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	55	theme	nanospun	1463:1470	arg1	bone					1524:1527	a new experimental bone substitute bioactive material	1505:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	56	theme	experimental	1511:1522	arg1	mesh					1491:1494	hybrid nanospun scaffold composite mesh	1456:1494	hybrid nanospun scaffold composite mesh	1456:1494	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	56	theme	experimental	1511:1522	arg1	bone					1524:1527	a new experimental bone substitute bioactive material	1505:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	4	57	theme	pulp	731:734	arg1	cells					741:745	dental pulp stem cells	724:745	dental pulp stem cells	724:745	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	9	58	from	bone	1524:1527	arg1	practice					1577:1584	future medical practice	1562:1584	future medical practice	1562:1584	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	5	59	from	defects	864:870	arg1	rats					875:878	rats	875:878	rats	875:878	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	8	60	theme	cross-linking	1304:1316	arg1	morphology					1270:1279	the morphology	1266:1279	the morphology of the mesh because of cross-linking	1266:1316	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	2	61	theme	phosphate-modified	389:406	arg1	aerogel					408:414	β-tricalcium phosphate-modified aerogel	376:414	β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions	376:541	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	0	62	theme	Bone	94:97	arg1	Regeneration					99:110	Bone Regeneration	94:110	Bone Regeneration	94:110	β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration.
37108742	2	63	theme	present	290:296	arg1	study					298:302	The present study	286:302	The present study	286:302	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	1	64	theme	nanofiber-based	223:237	arg1	delivery					244:251	nanofiber-based drug delivery	223:251	nanofiber-based drug delivery	223:251	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	9	65	theme	future	1562:1567	arg1	practice					1577:1584	future medical practice	1562:1584	future medical practice	1562:1584	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	2	66	contain	containing	416:425	arg1	aerogel					408:414	β-tricalcium phosphate-modified aerogel	376:414	β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions	376:541	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	66	contain	containing	416:425	arg2	meshes					462:467	polyvinyl alcohol/chitosan fibrous meshes	427:467	polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration	427:503	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	66	contain	containing	416:425	arg2	BTCP-AE-FMs					470:480	BTCP-AE-FMs	470:480	BTCP-AE-FMs	470:480	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	6	67	theme	standardized	1017:1028	arg1	SUVs					1045:1048	SUVs	1045:1048	SUVs	1045:1048	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	6	67	theme	standardized	1017:1028	arg1	values					1037:1042	18F-sodium fluoride standardized uptake values	997:1042	18F-sodium fluoride standardized uptake values (SUVs)	997:1049	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	3	68	from	1.7°	670:673	arg1	media					633:637	aqueous media	625:637	aqueous media	625:637	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	4	69	theme	electron	819:826	arg1	microscope					828:837	a scanning electron microscope	808:837	a scanning electron microscope	808:837	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	9	70	dep	bone	1524:1527	arg1	substitute					1529:1538	substitute	1529:1538	substitute	1529:1538	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	9	70	dep	bone	1524:1527	arg1	material					1550:1557	bioactive material	1540:1557	a new experimental bone substitute bioactive material	1505:1557	Our experiments proved that hybrid nanospun scaffold composite mesh could be a new experimental bone substitute bioactive material in future medical practice.
37108742	0	71	theme	Phosphate-Modified	13:30	arg1	Aerogel					32:38	β-Tricalcium Phosphate-Modified Aerogel	0:38	β-Tricalcium Phosphate-Modified Aerogel	0:38	β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration.
37108742	6	72	theme	alcohol/chitosan	1088:1103	arg1	FMs					1121:1123	FMs	1121:1123	FMs	1121:1123	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	6	72	theme	alcohol/chitosan	1088:1103	arg1	meshes					1113:1118	polyvinyl alcohol/chitosan fibrous meshes	1078:1118	polyvinyl alcohol/chitosan fibrous meshes (FMs)	1078:1124	PET imaging using 18F-sodium fluoride standardized uptake values (SUVs) detected 7.40 ± 1.03 using polyvinyl alcohol/chitosan fibrous meshes (FMs) while 10.72 ± 1.11 with BTCP-AE-FMs after 6 months.
37108742	2	73	theme	in	524:525	arg1	conditions					532:541	in vitro and in vivo conditions	511:541	in vitro and in vivo conditions	511:541	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	5	74	from	influence	937:945	arg1	regeneration					965:976	bone regeneration	960:976	bone regeneration	960:976	Critical-size calvarial defects in rats were performed as in vivo experiments to investigate the influence of meshes on bone regeneration.
37108742	8	75	theme	hydrophilic	1387:1397	arg1	character					1417:1425	hydrophilic and biocompatible character	1387:1425	hydrophilic and biocompatible character	1387:1425	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	3	76	theme	mesh	548:551	arg1	properties					569:578	The mesh physicochemical properties	544:578	The mesh physicochemical properties	544:578	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
37108742	0	77	theme	Hybrid	64:69	arg1	Scaffolds					80:88	PVA/Chitosan Hybrid Nanospun Scaffolds	51:88	PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration	51:110	β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration.
37108742	8	78	theme	biocompatible	1403:1415	arg1	character					1417:1425	hydrophilic and biocompatible character	1387:1425	hydrophilic and biocompatible character	1387:1425	Despite a slight change in the morphology of the mesh because of cross-linking, the BTCP-AE-FM basically retained its fibrous, porous structure and hydrophilic and biocompatible character.
37108742	2	79	theme	bone	487:490	arg1	regeneration					492:503	bone regeneration	487:503	bone regeneration	487:503	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	80	theme	fibrous	454:460	arg1	BTCP-AE-FMs					470:480	BTCP-AE-FMs	470:480	BTCP-AE-FMs	470:480	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	80	theme	fibrous	454:460	arg1	meshes					462:467	polyvinyl alcohol/chitosan fibrous meshes	427:467	polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration	427:503	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	81	theme	aerogel	408:414	arg1	suitability					361:371	suitability	361:371	suitability	361:371	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	2	81	theme	aerogel	408:414	arg1	preparation					345:355	the electrospinning preparation	325:355	the electrospinning preparation	325:355	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	4	82	theme	alamarBlue	782:791	arg1	assay					793:797	an alamarBlue assay	779:797	an alamarBlue assay	779:797	The viability of dental pulp stem cells on the BTCP-AE-FM was proven by an alamarBlue assay and with a scanning electron microscope.
37108742	2	83	theme	β-tricalcium	376:387	arg1	aerogel					408:414	β-tricalcium phosphate-modified aerogel	376:414	β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions	376:541	The present study aimed to demonstrate the electrospinning preparation and suitability of β-tricalcium phosphate-modified aerogel containing polyvinyl alcohol/chitosan fibrous meshes (BTCP-AE-FMs) for bone regeneration under in vitro and in vivo conditions.
37108742	1	84	theme	engineering	263:273	arg1	scaffolds					275:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	nanofiber-based drug delivery or tissue engineering scaffolds	223:283	Electrospinning has recently been recognized as a potential method for use in biomedical applications such as nanofiber-based drug delivery or tissue engineering scaffolds.
37108742	3	85	theme	nm	600:601	arg1	structure					611:619	50 nm fibrous structure	597:619	a 147 ± 50 nm fibrous structure	589:619	The mesh physicochemical properties included a 147 ± 50 nm fibrous structure, in aqueous media the contact angles were 64.1 ± 1.7°, and it released Ca, P, and Si.
35451731	0	0	theme	agro-industrial	89:103	arg1	biomasses					121:129	agro-industrial lignocellulosic biomasses	89:129	agro-industrial lignocellulosic biomasses	89:129	Evaluation of endoglucanase and xylanase production by Aspergillus tamarii cultivated in agro-industrial lignocellulosic biomasses.
35451731	5	1	theme	0.1	841:843	arg1	%					844:844	%	844:844	%	844:844	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	8	2	theme	negative	1351:1358	arg1	effect					1360:1365	a negative effect	1349:1365	a negative effect on xylanases	1349:1378	CuSO4 supplementation was advantageous for endoglucanases, increasing their activity, and it had a negative effect on xylanases.
35451731	8	3	contain	had	1345:1347	arg1	it					1342:1343	it	1342:1343	it	1342:1343	CuSO4 supplementation was advantageous for endoglucanases, increasing their activity, and it had a negative effect on xylanases.
35451731	8	3	contain	had	1345:1347	arg2	effect					1360:1365	a negative effect	1349:1365	a negative effect on xylanases	1349:1378	CuSO4 supplementation was advantageous for endoglucanases, increasing their activity, and it had a negative effect on xylanases.
35451731	5	4	theme	%	844:844	arg1	source					882:887	the only nitrogen source	864:887	the only nitrogen source	864:887	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	5	4	theme	%	844:844	arg1	tryptone					852:859	0.1% (w/v) tryptone	841:859	0.1% (w/v) tryptone	841:859	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	11	5	theme	induction	1724:1732	arg1	effect					1734:1739	the biomass induction effect	1712:1739	the biomass induction effect	1712:1739	More experimental studies are required to investigate how the biomass induction effect impacts enzyme production.
35451731	6	6	used	used	1043:1046	arg2	design					1032:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	10	7	theme	clean	1482:1486	arg1	residue					1495:1501	the clean cotton residue	1478:1501	the clean cotton residue	1478:1501	For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively.
35451731	1	8	theme	industrial	182:191	arg1	interest					193:200	industrial interest	182:200	industrial interest	182:200	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	1	9	from	tamarii	360:366	arg1	production					302:311	the production	298:311	the production of endoglucanase and xylanase from Aspergillus tamarii	298:366	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	6	10	theme	tryptone	1098:1105	arg1	influence					1085:1093	the simultaneous influence	1068:1093	the simultaneous influence of tryptone and CuSO4 on enzyme activity	1068:1134	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	4	11	theme	time-course	652:662	arg1	profile					664:670	A time-course profile	650:670	A time-course profile	650:670	A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase.
35451731	0	12	theme	lignocellulosic	105:119	arg1	biomasses					121:129	agro-industrial lignocellulosic biomasses	89:129	agro-industrial lignocellulosic biomasses	89:129	Evaluation of endoglucanase and xylanase production by Aspergillus tamarii cultivated in agro-industrial lignocellulosic biomasses.
35451731	10	13	theme	experimental	1508:1519	arg1	able					1532:1535	able	1532:1535	able	1532:1535	For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively.
35451731	10	13	theme	experimental	1508:1519	arg1	design					1521:1526	the experimental design	1504:1526	the experimental design	1504:1526	For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively.
35451731	3	14	theme	increased	592:600	arg1	recalcitrance					610:622	increased biomass recalcitrance	592:622	increased biomass recalcitrance yielding higher activity	592:647	The enzymatic profile could be associated with the biomass complexity, with increased biomass recalcitrance yielding higher activity.
35451731	1	15	theme	endoglucanase	316:328	arg1	production					302:311	the production	298:311	the production of endoglucanase and xylanase from Aspergillus tamarii	298:366	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	5	16	theme	12 µmol/L	893:901	arg1	addition					909:916	12 µmol/L CuSO4 addition	893:916	12 µmol/L CuSO4 addition	893:916	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	10	17	theme	highest	1550:1556	arg1	activity					1565:1572	the highest enzyme activity	1546:1572	the highest enzyme activity for endoglucanase and xylanase	1546:1603	For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively.
35451731	8	18	from	effect	1360:1365	arg1	xylanases					1370:1378	xylanases	1370:1378	xylanases	1370:1378	CuSO4 supplementation was advantageous for endoglucanases, increasing their activity, and it had a negative effect on xylanases.
35451731	1	19	theme	interest	193:200	arg1	enzymes					171:177	enzymes	171:177	enzymes of industrial interest	171:200	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	8	20	theme	CuSO4	1252:1256	arg1	supplementation					1258:1272	CuSO4 supplementation	1252:1272	CuSO4 supplementation	1252:1272	CuSO4 supplementation was advantageous for endoglucanases, increasing their activity, and it had a negative effect on xylanases.
35451731	9	21	theme	experimental	1398:1409	arg1	design					1411:1416	the experimental design	1394:1416	the experimental design	1394:1416	But overall, the experimental design increased the enzymatic activity of all biomasses used.
35451731	7	22	theme	enzymatic	1164:1172	arg1	activity					1174:1181	enzymatic activity	1164:1181	enzymatic activity	1164:1181	Tryptone strongly affected enzymatic activity, decreasing endoglucanase activity but increasing xylanase activity.
35451731	11	23	theme	biomass	1716:1722	arg1	effect					1734:1739	the biomass induction effect	1712:1739	the biomass induction effect	1712:1739	More experimental studies are required to investigate how the biomass induction effect impacts enzyme production.
35451731	11	24	theme	experimental	1659:1670	arg1	studies					1672:1678	More experimental studies	1654:1678	More experimental studies	1654:1678	More experimental studies are required to investigate how the biomass induction effect impacts enzyme production.
35451731	6	25	theme	enzyme	1120:1125	arg1	activity					1127:1134	enzyme activity	1120:1134	enzyme activity	1120:1134	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	2	26	theme	agricultural	483:494	arg1	biomasses					496:504	various agricultural biomasses	475:504	various agricultural biomasses	475:504	CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done.
35451731	7	27	theme	xylanase	1233:1240	arg1	activity					1242:1249	xylanase activity	1233:1249	xylanase activity	1233:1249	Tryptone strongly affected enzymatic activity, decreasing endoglucanase activity but increasing xylanase activity.
35451731	2	28	theme	CAZymes	369:375	arg1	domains					377:383	CAZymes domains	369:383	CAZymes domains	369:383	CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done.
35451731	5	29	theme	overall	925:931	arg1	effect					942:947	an overall positive effect	922:947	an overall positive effect	922:947	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	2	30	theme	various	475:481	arg1	biomasses					496:504	various agricultural biomasses	475:504	various agricultural biomasses	475:504	CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done.
35451731	1	31	theme	xylanase	334:341	arg1	production					302:311	the production	298:311	the production of endoglucanase and xylanase from Aspergillus tamarii	298:366	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	5	32	theme	protein	979:985	arg1	production					987:996	protein production	979:996	protein production	979:996	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	3	33	theme	biomass	602:608	arg1	recalcitrance					610:622	increased biomass recalcitrance	592:622	increased biomass recalcitrance yielding higher activity	592:647	The enzymatic profile could be associated with the biomass complexity, with increased biomass recalcitrance yielding higher activity.
35451731	2	34	theme	tamarii	464:470	arg1	potential					448:456	the enzymatic potential	434:456	the enzymatic potential of A. tamarii	434:470	CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done.
35451731	0	35	theme	endoglucanase	14:26	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of endoglucanase and xylanase production by Aspergillus tamarii	0:73	Evaluation of endoglucanase and xylanase production by Aspergillus tamarii cultivated in agro-industrial lignocellulosic biomasses.
35451731	6	36	theme	central	1014:1020	arg1	design					1032:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	5	37	dep	%	844:844	arg1	w/v					847:849	w/v	847:849	w/v	847:849	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	4	38	theme	sugarcane	749:757	arg1	bagasse					759:765	sugarcane bagasse	749:765	sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase	749:832	A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase.
35451731	4	39	theme	cultivation	688:698	arg1	48 h					680:683	48 h	680:683	48 h of cultivation	680:698	A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase.
35451731	4	39	theme	cultivation	688:698	arg1	period					712:717	the best period	703:717	the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase	703:832	A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase.
35451731	0	40	theme	production	41:50	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of endoglucanase and xylanase production by Aspergillus tamarii	0:73	Evaluation of endoglucanase and xylanase production by Aspergillus tamarii cultivated in agro-industrial lignocellulosic biomasses.
35451731	6	41	theme	factorial	1004:1012	arg1	design					1032:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	4	42	theme	best	707:710	arg1	48 h					680:683	48 h	680:683	48 h of cultivation	680:698	A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase.
35451731	4	42	theme	best	707:710	arg1	period					712:717	the best period	703:717	the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase	703:832	A time-course profile defined 48 h of cultivation as the best period for cultivating A. tamarii in sugarcane bagasse reached 12.05 IU/mg for endoglucanase and 74.86 IU/mg for xylanase.
35451731	3	43	theme	higher	633:638	arg1	activity					640:647	higher activity	633:647	higher activity	633:647	The enzymatic profile could be associated with the biomass complexity, with increased biomass recalcitrance yielding higher activity.
35451731	2	44	theme	enzymatic	438:446	arg1	potential					448:456	the enzymatic potential	434:456	the enzymatic potential of A. tamarii	434:470	CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done.
35451731	0	45	theme	xylanase	32:39	arg1	production					41:50	xylanase production	32:50	xylanase production	32:50	Evaluation of endoglucanase and xylanase production by Aspergillus tamarii cultivated in agro-industrial lignocellulosic biomasses.
35451731	5	46	theme	only	868:871	arg1	source					882:887	the only nitrogen source	864:887	the only nitrogen source	864:887	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	5	46	theme	only	868:871	arg1	tryptone					852:859	0.1% (w/v) tryptone	841:859	0.1% (w/v) tryptone	841:859	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	5	47	theme	CuSO4	903:907	arg1	addition					909:916	12 µmol/L CuSO4 addition	893:916	12 µmol/L CuSO4 addition	893:916	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	2	48	theme	potential	448:456	arg1	screening					421:429	a screening	419:429	a screening of the enzymatic potential of A. tamarii in various agricultural biomasses	419:504	CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done.
35451731	7	49	theme	endoglucanase	1195:1207	arg1	activity					1209:1216	endoglucanase activity	1195:1216	endoglucanase activity	1195:1216	Tryptone strongly affected enzymatic activity, decreasing endoglucanase activity but increasing xylanase activity.
35451731	1	50	with	microorganisms	207:220	arg1	potential					244:252	biotechnological potential	227:252	biotechnological potential using lignocellulosic biomass	227:282	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	6	51	theme	composite	1022:1030	arg1	design					1032:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design	999:1037	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	2	52	from	screening	421:429	arg1	biomasses					496:504	various agricultural biomasses	475:504	various agricultural biomasses	475:504	CAZymes domains were evaluated in the genome, and a screening of the enzymatic potential of A. tamarii in various agricultural biomasses was done.
35451731	5	53	theme	enzymatic	956:964	arg1	activity					966:973	the enzymatic activity	952:973	the enzymatic activity	952:973	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	10	54	theme	enzyme	1558:1563	arg1	activity					1565:1572	the highest enzyme activity	1546:1572	the highest enzyme activity for endoglucanase and xylanase	1546:1603	For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively.
35451731	9	55	theme	biomasses	1458:1466	arg1	activity					1442:1449	the enzymatic activity	1428:1449	the enzymatic activity of all biomasses used	1428:1471	But overall, the experimental design increased the enzymatic activity of all biomasses used.
35451731	5	56	dep	had	918:920	arg1	Using					835:839	Using	835:839	Using 0.1% (w/v) tryptone as the only nitrogen source	835:887	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	6	57	from	influence	1085:1093	arg1	activity					1127:1134	enzyme activity	1120:1134	enzyme activity	1120:1134	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	10	58	theme	cotton	1488:1493	arg1	residue					1495:1501	the clean cotton residue	1478:1501	the clean cotton residue	1478:1501	For the clean cotton residue, the experimental design was able to reach the highest enzyme activity for endoglucanase and xylanase, with 1.195 IU/mL and 6.353 IU/mL, respectively.
35451731	9	59	theme	enzymatic	1432:1440	arg1	activity					1442:1449	the enzymatic activity	1428:1449	the enzymatic activity of all biomasses used	1428:1471	But overall, the experimental design increased the enzymatic activity of all biomasses used.
35451731	3	60	theme	enzymatic	520:528	arg1	profile					530:536	The enzymatic profile	516:536	The enzymatic profile	516:536	The enzymatic profile could be associated with the biomass complexity, with increased biomass recalcitrance yielding higher activity.
35451731	6	61	theme	CuSO4	1111:1115	arg1	influence					1085:1093	the simultaneous influence	1068:1093	the simultaneous influence of tryptone and CuSO4 on enzyme activity	1068:1134	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	1	62	from	microorganisms	207:220	arg1	production					157:166	the production	153:166	the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass	153:282	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	3	63	theme	biomass	567:573	arg1	complexity					575:584	the biomass complexity	563:584	the biomass complexity	563:584	The enzymatic profile could be associated with the biomass complexity, with increased biomass recalcitrance yielding higher activity.
35451731	11	64	theme	enzyme	1749:1754	arg1	production					1756:1765	enzyme production	1749:1765	enzyme production	1749:1765	More experimental studies are required to investigate how the biomass induction effect impacts enzyme production.
35451731	6	65	theme	simultaneous	1072:1083	arg1	influence					1085:1093	the simultaneous influence	1068:1093	the simultaneous influence of tryptone and CuSO4 on enzyme activity	1068:1134	A 22 factorial central composite design was used then to investigate the simultaneous influence of tryptone and CuSO4 on enzyme activity.
35451731	1	66	theme	lignocellulosic	260:274	arg1	biomass					276:282	lignocellulosic biomass	260:282	lignocellulosic biomass	260:282	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	1	67	theme	biotechnological	227:242	arg1	potential					244:252	biotechnological potential	227:252	biotechnological potential using lignocellulosic biomass	227:282	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
35451731	5	68	theme	positive	933:940	arg1	effect					942:947	an overall positive effect	922:947	an overall positive effect	922:947	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	7	69	dep	affected	1155:1162	arg1	increasing					1222:1231	increasing	1222:1231	increasing xylanase activity	1222:1249	Tryptone strongly affected enzymatic activity, decreasing endoglucanase activity but increasing xylanase activity.
35451731	7	69	dep	affected	1155:1162	arg1	decreasing					1184:1193	decreasing	1184:1193	decreasing endoglucanase activity	1184:1216	Tryptone strongly affected enzymatic activity, decreasing endoglucanase activity but increasing xylanase activity.
35451731	5	70	theme	nitrogen	873:880	arg1	source					882:887	the only nitrogen source	864:887	the only nitrogen source	864:887	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	5	70	theme	nitrogen	873:880	arg1	tryptone					852:859	0.1% (w/v) tryptone	841:859	0.1% (w/v) tryptone	841:859	Using 0.1% (w/v) tryptone as the only nitrogen source and 12 µmol/L CuSO4 addition had an overall positive effect on the enzymatic activity and protein production.
35451731	1	71	theme	enzymes	171:177	arg1	production					157:166	the production	153:166	the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass	153:282	To better understand the production of enzymes of industrial interest from microorganisms with biotechnological potential using lignocellulosic biomass, we evaluated the production of endoglucanase and xylanase from Aspergillus tamarii.
36495667	4	0	from	role	556:559	arg1	metabolites					601:611	endogenous metabolites	590:611	endogenous metabolites	590:611	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	0	from	role	556:559	arg1	microbiota					621:630	gut microbiota	617:630	gut microbiota	617:630	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	11	1	theme	A.	1680:1681	arg1	supplementation					1695:1709	A. muciniphila supplementation	1680:1709	A. muciniphila supplementation	1680:1709	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	11	1	theme	A.	1680:1681	arg1	option					1744:1749	a promising therapeutic option	1720:1749	a promising therapeutic option for lung diseases	1720:1767	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	7	2	theme	enriched	967:974	arg1	muciniphila					988:998	the enriched Akkermansia muciniphila	963:998	the enriched Akkermansia muciniphila	963:998	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	4	3	theme	lung	664:667	arg1	ALI					677:679	ALI	677:679	ALI	677:679	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	3	theme	lung	664:667	arg1	injury					669:674	LPS-induced acute lung injury	646:674	LPS-induced acute lung injury (ALI)	646:680	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	10	4	theme	current	1521:1527	arg1	study					1529:1533	the current study	1517:1533	the current study	1517:1533	In conclusion, the current study demonstrated for the first time that KQ may act on the A. muciniphila abundance, regulate IDO1 activity, and thus ameliorate ALI.
36495667	10	5	dep	A.	1590:1591	arg1	muciniphila					1593:1603	muciniphila	1593:1603	muciniphila	1593:1603	In conclusion, the current study demonstrated for the first time that KQ may act on the A. muciniphila abundance, regulate IDO1 activity, and thus ameliorate ALI.
36495667	8	6	theme	beneficial	1200:1209	arg1	effects					1225:1231	the beneficial and causative effects	1196:1231	the beneficial and causative effects of A. muciniphila	1196:1249	Furthermore, the beneficial and causative effects of A. muciniphila were confirmed by antibiotic and microbial intervention experiments.
36495667	4	7	theme	acute	658:662	arg1	ALI					677:679	ALI	677:679	ALI	677:679	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	7	theme	acute	658:662	arg1	injury					669:674	LPS-induced acute lung injury	646:674	LPS-induced acute lung injury (ALI)	646:680	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	11	8	theme	gut-lung	1781:1788	arg1	axis					1790:1793	the gut-lung axis	1777:1793	the gut-lung axis	1777:1793	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	7	9	theme	R2	1170:1171	arg1	p = 0.0214					1158:1167	p = 0.0214	1158:1167	p = 0.0214	1158:1167	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	7	9	theme	R2	1170:1171	arg1	=0.7712					1173:1179	R2 =0.7712	1170:1179	R2 =0.7712	1170:1179	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	3	10	theme	action	501:506	arg1	mechanism					488:496	its mechanism	484:496	its mechanism of action	484:506	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	0	11	theme	Akkermansia	111:121	arg1	muciniphila					123:133	Akkermansia muciniphila	111:133	Akkermansia muciniphila	111:133	Kuqin ameliorates Lipopolysaccharide-induced acute lung injury by regulating indoleamine 2,3-dioxygenase 1 and Akkermansia muciniphila.
36495667	3	12	contain	has	401:403	arg1	KQ					398:399	KQ	398:399	KQ	398:399	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	3	12	contain	has	401:403	arg2	effect					414:419	a better effect	405:419	a better effect than ZQ	405:427	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	9	13	theme	tryptophan	1470:1479	arg1	imbalance					1491:1499	tryptophan metabolic imbalance	1470:1499	tryptophan metabolic imbalance	1470:1499	Live and pasteurized A. muciniphila, both supplements could ameliorate the inflammatory response and down-regulate IDO1 expression, thereby restoring tryptophan metabolic imbalance.
36495667	9	14	theme	IDO1	1435:1438	arg1	expression					1440:1449	IDO1 expression	1435:1449	IDO1 expression	1435:1449	Live and pasteurized A. muciniphila, both supplements could ameliorate the inflammatory response and down-regulate IDO1 expression, thereby restoring tryptophan metabolic imbalance.
36495667	7	15	theme	indoleamine	1111:1121	arg1	2,3-dioxygenase					1123:1137	indoleamine 2,3-dioxygenase 1	1111:1139	indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712)	1111:1180	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	7	15	theme	indoleamine	1111:1121	arg1	IDO1					1142:1145	IDO1	1142:1145	IDO1	1142:1145	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	4	16	theme	alterations	575:585	arg1	role					556:559	the role	552:559	the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI)	552:680	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	17	from	microbiota	621:630	arg1	role					556:559	the role	552:559	the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI)	552:680	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	18	theme	gut	617:619	arg1	microbiota					621:630	gut microbiota	617:630	gut microbiota	617:630	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	10	19	theme	A.	1590:1591	arg1	abundance					1605:1613	the A. muciniphila abundance	1586:1613	the A. muciniphila abundance	1586:1613	In conclusion, the current study demonstrated for the first time that KQ may act on the A. muciniphila abundance, regulate IDO1 activity, and thus ameliorate ALI.
36495667	11	20	theme	muciniphila	1683:1693	arg1	supplementation					1695:1709	A. muciniphila supplementation	1680:1709	A. muciniphila supplementation	1680:1709	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	11	20	theme	muciniphila	1683:1693	arg1	option					1744:1749	a promising therapeutic option	1720:1749	a promising therapeutic option for lung diseases	1720:1767	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	7	21	theme	2,3-dioxygenase	1123:1137	arg1	activity					1148:1155	indoleamine 2,3-dioxygenase 1 (IDO1) activity	1111:1155	indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712)	1111:1180	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	7	21	theme	2,3-dioxygenase	1123:1137	arg1	enzyme					1066:1071	the rate-limiting enzyme	1048:1071	the rate-limiting enzyme of the tryptophan/kynurenine pathway	1048:1108	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	2	22	theme	commercial	283:292	arg1	Kuqin					319:323	Kuqin	319:323	Kuqin (KQ)	319:328	The two main commercial specifications of SR are Kuqin (KQ) and Ziqin (ZQ).
36495667	2	22	theme	commercial	283:292	arg1	specifications					294:307	The two main commercial specifications	270:307	The two main commercial specifications of SR	270:313	The two main commercial specifications of SR are Kuqin (KQ) and Ziqin (ZQ).
36495667	9	23	theme	inflammatory	1395:1406	arg1	response					1408:1415	the inflammatory response	1391:1415	the inflammatory response	1391:1415	Live and pasteurized A. muciniphila, both supplements could ameliorate the inflammatory response and down-regulate IDO1 expression, thereby restoring tryptophan metabolic imbalance.
36495667	3	24	theme	Chinese	371:377	arg1	medicine					379:386	traditional Chinese medicine	359:386	traditional Chinese medicine theories	359:395	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	7	25	theme	pathway	1102:1108	arg1	activity					1148:1155	indoleamine 2,3-dioxygenase 1 (IDO1) activity	1111:1155	indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712)	1111:1180	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	7	25	theme	pathway	1102:1108	arg1	enzyme					1066:1071	the rate-limiting enzyme	1048:1071	the rate-limiting enzyme of the tryptophan/kynurenine pathway	1048:1108	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	10	26	theme	IDO1	1625:1628	arg1	activity					1630:1637	IDO1 activity	1625:1637	IDO1 activity	1625:1637	In conclusion, the current study demonstrated for the first time that KQ may act on the A. muciniphila abundance, regulate IDO1 activity, and thus ameliorate ALI.
36495667	3	27	theme	medicine	379:386	arg1	theories					388:395	traditional Chinese medicine theories	359:395	traditional Chinese medicine theories	359:395	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	8	28	theme	muciniphila	1239:1249	arg1	effects					1225:1231	the beneficial and causative effects	1196:1231	the beneficial and causative effects of A. muciniphila	1196:1249	Furthermore, the beneficial and causative effects of A. muciniphila were confirmed by antibiotic and microbial intervention experiments.
36495667	7	29	dep	activity	1148:1155	arg1	p = 0.0214					1158:1167	p = 0.0214	1158:1167	p = 0.0214	1158:1167	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	7	29	dep	activity	1148:1155	arg1	=0.7712					1173:1179	R2 =0.7712	1170:1179	R2 =0.7712	1170:1179	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	4	30	from	metabolites	601:611	arg1	role					556:559	the role	552:559	the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI)	552:680	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	3	31	theme	better	407:412	arg1	effect					414:419	a better effect	405:419	a better effect than ZQ	405:427	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	0	32	theme	acute	45:49	arg1	injury					56:61	Lipopolysaccharide-induced acute lung injury	18:61	Lipopolysaccharide-induced acute lung injury	18:61	Kuqin ameliorates Lipopolysaccharide-induced acute lung injury by regulating indoleamine 2,3-dioxygenase 1 and Akkermansia muciniphila.
36495667	5	33	theme	organ	775:779	arg1	properties					792:801	satisfactory organ protection properties	762:801	satisfactory organ protection properties	762:801	KQ treatment ameliorated lung injury more effectively than ZQ and demonstrated satisfactory organ protection properties.
36495667	9	34	theme	metabolic	1481:1489	arg1	imbalance					1491:1499	tryptophan metabolic imbalance	1470:1499	tryptophan metabolic imbalance	1470:1499	Live and pasteurized A. muciniphila, both supplements could ameliorate the inflammatory response and down-regulate IDO1 expression, thereby restoring tryptophan metabolic imbalance.
36495667	8	35	theme	antibiotic	1269:1278	arg1	experiments					1307:1317	antibiotic and microbial intervention experiments	1269:1317	antibiotic and microbial intervention experiments	1269:1317	Furthermore, the beneficial and causative effects of A. muciniphila were confirmed by antibiotic and microbial intervention experiments.
36495667	3	36	theme	traditional	359:369	arg1	medicine					379:386	traditional Chinese medicine	359:386	traditional Chinese medicine theories	359:395	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	7	37	theme	tryptophan/kynurenine	1080:1100	arg1	pathway					1102:1108	the tryptophan/kynurenine pathway	1076:1108	the tryptophan/kynurenine pathway	1076:1108	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	11	38	theme	therapeutic	1732:1742	arg1	supplementation					1695:1709	A. muciniphila supplementation	1680:1709	A. muciniphila supplementation	1680:1709	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	11	38	theme	therapeutic	1732:1742	arg1	option					1744:1749	a promising therapeutic option	1720:1749	a promising therapeutic option for lung diseases	1720:1767	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	1	39	theme	Scutellariae	136:147	arg1	SR					156:157	SR	156:157	SR	156:157	Scutellariae radix (SR) has been proven to be highly effective in treating inflammation because of its superior medicinal properties.
36495667	1	39	theme	Scutellariae	136:147	arg1	radix					149:153	Scutellariae radix	136:153	Scutellariae radix (SR)	136:158	Scutellariae radix (SR) has been proven to be highly effective in treating inflammation because of its superior medicinal properties.
36495667	3	40	theme	upper	443:447	arg1	heat					469:472	upper energizer lung damp heat	443:472	upper energizer lung damp heat	443:472	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	8	41	theme	causative	1215:1223	arg1	effects					1225:1231	the beneficial and causative effects	1196:1231	the beneficial and causative effects of A. muciniphila	1196:1249	Furthermore, the beneficial and causative effects of A. muciniphila were confirmed by antibiotic and microbial intervention experiments.
36495667	6	42	theme	metabolite	841:850	arg1	abnormalities					852:864	the tryptophan metabolite abnormalities	826:864	the tryptophan metabolite abnormalities in ALI	826:871	KQ treatment reversed the tryptophan metabolite abnormalities in ALI and reshaped the composition of gut microbial communities.
36495667	4	43	from	alterations	575:585	arg1	metabolites					601:611	endogenous metabolites	590:611	endogenous metabolites	590:611	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	43	from	alterations	575:585	arg1	microbiota					621:630	gut microbiota	617:630	gut microbiota	617:630	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	8	44	theme	microbial	1284:1292	arg1	intervention					1294:1305	microbial intervention	1284:1305	microbial intervention	1284:1305	Furthermore, the beneficial and causative effects of A. muciniphila were confirmed by antibiotic and microbial intervention experiments.
36495667	0	45	theme	lung	51:54	arg1	injury					56:61	Lipopolysaccharide-induced acute lung injury	18:61	Lipopolysaccharide-induced acute lung injury	18:61	Kuqin ameliorates Lipopolysaccharide-induced acute lung injury by regulating indoleamine 2,3-dioxygenase 1 and Akkermansia muciniphila.
36495667	6	46	from	abnormalities	852:864	arg1	ALI					869:871	ALI	869:871	ALI	869:871	KQ treatment reversed the tryptophan metabolite abnormalities in ALI and reshaped the composition of gut microbial communities.
36495667	6	47	theme	tryptophan	830:839	arg1	abnormalities					852:864	the tryptophan metabolite abnormalities	826:864	the tryptophan metabolite abnormalities in ALI	826:871	KQ treatment reversed the tryptophan metabolite abnormalities in ALI and reshaped the composition of gut microbial communities.
36495667	4	48	theme	KQ-induced	564:573	arg1	alterations					575:585	KQ-induced alterations	564:585	KQ-induced alterations in endogenous metabolites and gut microbiota	564:630	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	49	theme	endogenous	590:599	arg1	metabolites					601:611	endogenous metabolites	590:611	endogenous metabolites	590:611	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	9	50	dep	muciniphila	1344:1354	arg1	both					1357:1360	both	1357:1360	both	1357:1360	Live and pasteurized A. muciniphila, both supplements could ameliorate the inflammatory response and down-regulate IDO1 expression, thereby restoring tryptophan metabolic imbalance.
36495667	10	51	dep	act	1579:1581	arg1	ameliorate					1649:1658	ameliorate	1649:1658	ameliorate ALI	1649:1662	In conclusion, the current study demonstrated for the first time that KQ may act on the A. muciniphila abundance, regulate IDO1 activity, and thus ameliorate ALI.
36495667	11	52	theme	promising	1722:1730	arg1	supplementation					1695:1709	A. muciniphila supplementation	1680:1709	A. muciniphila supplementation	1680:1709	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	11	52	theme	promising	1722:1730	arg1	option					1744:1749	a promising therapeutic option	1720:1749	a promising therapeutic option for lung diseases	1720:1767	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	3	53	theme	damp	464:467	arg1	heat					469:472	upper energizer lung damp heat	443:472	upper energizer lung damp heat	443:472	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	6	54	theme	communities	919:929	arg1	composition					890:900	the composition	886:900	the composition of gut microbial communities	886:929	KQ treatment reversed the tryptophan metabolite abnormalities in ALI and reshaped the composition of gut microbial communities.
36495667	7	55	theme	rate-limiting	1052:1064	arg1	activity					1148:1155	indoleamine 2,3-dioxygenase 1 (IDO1) activity	1111:1155	indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712)	1111:1180	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	7	55	theme	rate-limiting	1052:1064	arg1	enzyme					1066:1071	the rate-limiting enzyme	1048:1071	the rate-limiting enzyme of the tryptophan/kynurenine pathway	1048:1108	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	5	56	theme	protection	781:790	arg1	properties					792:801	satisfactory organ protection properties	762:801	satisfactory organ protection properties	762:801	KQ treatment ameliorated lung injury more effectively than ZQ and demonstrated satisfactory organ protection properties.
36495667	8	57	theme	intervention	1294:1305	arg1	experiments					1307:1317	antibiotic and microbial intervention experiments	1269:1317	antibiotic and microbial intervention experiments	1269:1317	Furthermore, the beneficial and causative effects of A. muciniphila were confirmed by antibiotic and microbial intervention experiments.
36495667	6	58	theme	microbial	909:917	arg1	communities					919:929	gut microbial communities	905:929	gut microbial communities	905:929	KQ treatment reversed the tryptophan metabolite abnormalities in ALI and reshaped the composition of gut microbial communities.
36495667	2	59	theme	main	278:281	arg1	Kuqin					319:323	Kuqin	319:323	Kuqin (KQ)	319:328	The two main commercial specifications of SR are Kuqin (KQ) and Ziqin (ZQ).
36495667	2	59	theme	main	278:281	arg1	specifications					294:307	The two main commercial specifications	270:307	The two main commercial specifications of SR	270:313	The two main commercial specifications of SR are Kuqin (KQ) and Ziqin (ZQ).
36495667	3	60	theme	energizer	449:457	arg1	heat					469:472	upper energizer lung damp heat	443:472	upper energizer lung damp heat	443:472	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	10	61	theme	first	1556:1560	arg1	time					1562:1565	the first time	1552:1565	the first time	1552:1565	In conclusion, the current study demonstrated for the first time that KQ may act on the A. muciniphila abundance, regulate IDO1 activity, and thus ameliorate ALI.
36495667	6	62	theme	KQ	804:805	arg1	treatment					807:815	KQ treatment	804:815	KQ treatment	804:815	KQ treatment reversed the tryptophan metabolite abnormalities in ALI and reshaped the composition of gut microbial communities.
36495667	2	63	theme	SR	312:313	arg1	Kuqin					319:323	Kuqin	319:323	Kuqin (KQ)	319:328	The two main commercial specifications of SR are Kuqin (KQ) and Ziqin (ZQ).
36495667	2	63	theme	SR	312:313	arg1	specifications					294:307	The two main commercial specifications	270:307	The two main commercial specifications of SR	270:313	The two main commercial specifications of SR are Kuqin (KQ) and Ziqin (ZQ).
36495667	7	64	theme	Akkermansia	976:986	arg1	muciniphila					988:998	the enriched Akkermansia muciniphila	963:998	the enriched Akkermansia muciniphila	963:998	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	3	65	theme	lung	459:462	arg1	heat					469:472	upper energizer lung damp heat	443:472	upper energizer lung damp heat	443:472	According to traditional Chinese medicine theories, KQ has a better effect than ZQ on dispelling upper energizer lung damp heat, however, its mechanism of action is not known.
36495667	1	66	theme	superior	239:246	arg1	properties					258:267	its superior medicinal properties	235:267	its superior medicinal properties	235:267	Scutellariae radix (SR) has been proven to be highly effective in treating inflammation because of its superior medicinal properties.
36495667	5	67	theme	lung	708:711	arg1	injury					713:718	lung injury	708:718	lung injury	708:718	KQ treatment ameliorated lung injury more effectively than ZQ and demonstrated satisfactory organ protection properties.
36495667	11	68	theme	lung	1755:1758	arg1	diseases					1760:1767	lung diseases	1755:1767	lung diseases	1755:1767	Interestingly, A. muciniphila supplementation could be a promising therapeutic option for lung diseases through the gut-lung axis.
36495667	7	69	theme	muciniphila	988:998	arg1	abundance					950:958	the abundance	946:958	the abundance of the enriched Akkermansia muciniphila	946:998	Additionally, the abundance of the enriched Akkermansia muciniphila was significantly and inversely correlated with the rate-limiting enzyme of the tryptophan/kynurenine pathway, indoleamine 2,3-dioxygenase 1 (IDO1) activity (p = 0.0214, R2 =0.7712).
36495667	4	70	theme	LPS-induced	646:656	arg1	ALI					677:679	ALI	677:679	ALI	677:679	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	4	70	theme	LPS-induced	646:656	arg1	injury					669:674	LPS-induced acute lung injury	646:674	LPS-induced acute lung injury (ALI)	646:680	Thus, this study investigated the role of KQ-induced alterations in endogenous metabolites and gut microbiota in regulating LPS-induced acute lung injury (ALI).
36495667	1	71	theme	medicinal	248:256	arg1	properties					258:267	its superior medicinal properties	235:267	its superior medicinal properties	235:267	Scutellariae radix (SR) has been proven to be highly effective in treating inflammation because of its superior medicinal properties.
36495667	0	72	theme	indoleamine	77:87	arg1	2,3-dioxygenase					89:103	indoleamine 2,3-dioxygenase 1	77:105	indoleamine 2,3-dioxygenase 1	77:105	Kuqin ameliorates Lipopolysaccharide-induced acute lung injury by regulating indoleamine 2,3-dioxygenase 1 and Akkermansia muciniphila.
36495667	6	73	theme	gut	905:907	arg1	communities					919:929	gut microbial communities	905:929	gut microbial communities	905:929	KQ treatment reversed the tryptophan metabolite abnormalities in ALI and reshaped the composition of gut microbial communities.
36495667	0	74	theme	Lipopolysaccharide-induced	18:43	arg1	injury					56:61	Lipopolysaccharide-induced acute lung injury	18:61	Lipopolysaccharide-induced acute lung injury	18:61	Kuqin ameliorates Lipopolysaccharide-induced acute lung injury by regulating indoleamine 2,3-dioxygenase 1 and Akkermansia muciniphila.
36495667	5	75	theme	satisfactory	762:773	arg1	properties					792:801	satisfactory organ protection properties	762:801	satisfactory organ protection properties	762:801	KQ treatment ameliorated lung injury more effectively than ZQ and demonstrated satisfactory organ protection properties.
36495667	5	76	theme	KQ	683:684	arg1	treatment					686:694	KQ treatment	683:694	KQ treatment	683:694	KQ treatment ameliorated lung injury more effectively than ZQ and demonstrated satisfactory organ protection properties.
35149682	0	0	theme	cryo-electron	69:81	arg1	microscopy					83:92	cryo-electron microscopy	69:92	cryo-electron microscopy	69:92	Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy.
35149682	2	1	theme	healthy	296:302	arg1	people					304:309	healthy people	296:309	healthy people	296:309	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	1	2	theme	reproductive	205:216	arg1	losses					218:223	reproductive losses	205:223	reproductive losses	205:223	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	7	3	theme	E2	1016:1017	arg1	assembly					997:1004	the transmembrane assembly	979:1004	the transmembrane assembly of E1 and E2	979:1017	The S-acylation sites might be involved in stabilizing the transmembrane assembly of E1 and E2.
35149682	6	4	theme	immune	885:890	arg1	evasion					892:898	viral immune evasion	879:898	viral immune evasion	879:898	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	10	5	theme	vaccine	1354:1360	arg1	design					1373:1378	structure-based antiviral and vaccine screening, design, and optimization	1324:1396	design	1373:1378	The structural information will assist structure-based antiviral and vaccine screening, design, and optimization.
35149682	5	6	from	sites	801:805	arg1	E1					810:811	E1	810:811	E1	810:811	We have identified numerous glycosylation and S-acylation sites in E1 and E2.
35149682	5	6	from	sites	801:805	arg1	E2					817:818	E2	817:818	E2	817:818	We have identified numerous glycosylation and S-acylation sites in E1 and E2.
35149682	6	7	theme	viral	879:883	arg1	evasion					892:898	viral immune evasion	879:898	viral immune evasion	879:898	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	6	8	theme	possible	860:867	arg1	impact					869:874	a possible impact	858:874	a possible impact on viral immune evasion and host cell invasion	858:921	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	4	9	attach	present	591:597	arg2	we					588:589	we	588:589	we	588:589	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	9	attach	present	591:597	arg1	E2					739:740	E2	739:740	E2	739:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	9	attach	present	591:597	arg1	resolution					636:645	a resolution	634:645	a resolution of 2.8 Å with the atomic models of the capsid protein	634:699	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	9	attach	present	591:597	arg1	E1					732:733	E1	732:733	E1	732:733	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	9	attach	present	591:597	arg1	glycoproteins					718:730	the envelope glycoproteins	705:730	the envelope glycoproteins E1 and E2	705:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	7	10	theme	E1	1009:1010	arg1	assembly					997:1004	the transmembrane assembly	979:1004	the transmembrane assembly of E1 and E2	979:1017	The S-acylation sites might be involved in stabilizing the transmembrane assembly of E1 and E2.
35149682	5	11	theme	S-acylation	789:799	arg1	sites					801:805	numerous glycosylation and S-acylation sites	762:805	numerous glycosylation and S-acylation sites in E1 and E2	762:818	We have identified numerous glycosylation and S-acylation sites in E1 and E2.
35149682	8	12	theme	more	1145:1148	arg1	cholesterols					1150:1161	two more cholesterols	1141:1161	two more cholesterols surrounding the pocket	1141:1184	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	7	13	theme	transmembrane	983:995	arg1	assembly					997:1004	the transmembrane assembly	979:1004	the transmembrane assembly of E1 and E2	979:1017	The S-acylation sites might be involved in stabilizing the transmembrane assembly of E1 and E2.
35149682	2	14	theme	symptoms	345:352	arg1	reports					325:331	no reports	322:331	no reports of clinical symptoms associated with GETV	322:373	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	3	15	theme	vaccine	464:470	arg1	treatments					472:481	vaccine treatments	464:481	vaccine treatments	464:481	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	1	16	theme	genus	131:135	arg1	alphavirus					137:146	the genus alphavirus	127:146	the genus alphavirus	127:146	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	4	17	theme	protein	693:699	arg1	models					672:677	the atomic models	661:677	the atomic models of the capsid protein	661:699	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	10	18	theme	antiviral	1340:1348	arg1	design					1373:1378	structure-based antiviral and vaccine screening, design, and optimization	1324:1396	design	1373:1378	The structural information will assist structure-based antiviral and vaccine screening, design, and optimization.
35149682	4	19	theme	atomic	665:670	arg1	models					672:677	the atomic models	661:677	the atomic models of the capsid protein	661:699	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	1	20	theme	alphavirus	137:146	arg1	member					117:122	a member	115:122	a member of the genus alphavirus	115:146	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	1	20	theme	alphavirus	137:146	arg1	virus					101:105	Getah virus	95:105	Getah virus (GETV)	95:112	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	10	21	theme	structure-based	1324:1338	arg1	design					1373:1378	structure-based antiviral and vaccine screening, design, and optimization	1324:1396	design	1373:1378	The structural information will assist structure-based antiviral and vaccine screening, design, and optimization.
35149682	8	22	theme	phospholipid	1053:1064	arg1	molecule					1066:1073	a phospholipid molecule	1051:1073	a phospholipid molecule	1051:1073	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	9	23	from	pocket	1246:1251	arg1	membrane					1275:1282	the viral envelope membrane	1256:1282	the viral envelope membrane	1256:1282	The cholesterol and phospholipid stabilize the hydrophobic pocket in the viral envelope membrane.
35149682	5	24	theme	numerous	762:769	arg1	sites					801:805	numerous glycosylation and S-acylation sites	762:805	numerous glycosylation and S-acylation sites in E1 and E2	762:818	We have identified numerous glycosylation and S-acylation sites in E1 and E2.
35149682	0	25	theme	Getah	23:27	arg1	virus					29:33	infective Getah virus	13:33	infective Getah virus	13:33	Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy.
35149682	2	26	located	found	275:279	arg1	%					291:291	over 10%	284:291	over 10% of healthy people	284:309	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	2	26	located	found	275:279	arg1	people					304:309	healthy people	296:309	healthy people	296:309	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	2	26	located	found	275:279	arg2	antibodies					246:255	antibodies	246:255	antibodies to GETV	246:263	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	8	27	located	observed	1079:1086	arg1	addition					1023:1030	addition	1023:1030	addition	1023:1030	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	8	27	located	observed	1079:1086	arg1	pocket					1119:1124	a transmembrane hydrophobic pocket	1091:1124	a transmembrane hydrophobic pocket	1091:1124	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	8	27	located	observed	1079:1086	arg2	cholesterol					1035:1045	a cholesterol	1033:1045	a cholesterol	1033:1045	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	8	27	located	observed	1079:1086	arg2	molecule					1066:1073	a phospholipid molecule	1051:1073	a phospholipid molecule	1051:1073	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	1	28	theme	Getah	95:99	arg1	member					117:122	a member	115:122	a member of the genus alphavirus	115:146	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	1	28	theme	Getah	95:99	arg1	pathogen					169:176	a mosquito-borne pathogen	152:176	a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals	152:234	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	1	28	theme	Getah	95:99	arg1	GETV					108:111	GETV	108:111	GETV	108:111	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	1	28	theme	Getah	95:99	arg1	virus					101:105	Getah virus	95:105	Getah virus (GETV)	95:112	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	3	29	theme	pathological	395:406	arg1	unknown					439:445	unknown	439:445	unknown	439:445	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	3	29	theme	pathological	395:406	arg1	properties					408:417	The biological and pathological properties	376:417	The biological and pathological properties of GETV	376:425	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	0	30	theme	infective	13:21	arg1	virus					29:33	infective Getah virus	13:33	infective Getah virus	13:33	Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy.
35149682	3	31	theme	GETV	569:572	arg1	virion					574:579	the GETV virion	565:579	the GETV virion	565:579	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	3	32	theme	biological	380:389	arg1	unknown					439:445	unknown	439:445	unknown	439:445	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	3	32	theme	biological	380:389	arg1	properties					408:417	The biological and pathological properties	376:417	The biological and pathological properties of GETV	376:425	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	1	33	theme	mosquito-borne	154:167	arg1	pathogen					169:176	a mosquito-borne pathogen	152:176	a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals	152:234	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	1	33	theme	mosquito-borne	154:167	arg1	virus					101:105	Getah virus	95:105	Getah virus (GETV)	95:112	Getah virus (GETV), a member of the genus alphavirus, is a mosquito-borne pathogen that can cause pyrexia and reproductive losses in animals.
35149682	6	34	from	impact	869:874	arg1	evasion					892:898	viral immune evasion	879:898	viral immune evasion	879:898	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	6	34	from	impact	869:874	arg1	invasion					914:921	host cell invasion	904:921	host cell invasion	904:921	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	3	35	theme	virion	574:579	arg1	structure					552:560	the structure	548:560	the structure of the GETV virion	548:579	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	2	36	theme	clinical	336:343	arg1	symptoms					345:352	clinical symptoms	336:352	clinical symptoms associated with GETV	336:373	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	0	37	theme	virus	29:33	arg1	Structure					0:8	Structure	0:8	Structure of infective Getah virus at 2.8 Å resolution	0:53	Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy.
35149682	4	38	with	Å	654:654	arg1	models					672:677	the atomic models	661:677	the atomic models of the capsid protein	661:699	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	9	39	theme	hydrophobic	1234:1244	arg1	pocket					1246:1251	the hydrophobic pocket	1230:1251	the hydrophobic pocket in the viral envelope membrane	1230:1282	The cholesterol and phospholipid stabilize the hydrophobic pocket in the viral envelope membrane.
35149682	0	40	theme	Å	42:42	arg1	resolution					44:53	2.8 Å resolution	38:53	2.8 Å resolution	38:53	Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy.
35149682	6	41	theme	surface-exposed	825:839	arg1	glycans					841:847	The surface-exposed glycans	821:847	The surface-exposed glycans	821:847	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	7	42	theme	S-acylation	928:938	arg1	sites					940:944	The S-acylation sites	924:944	The S-acylation sites	924:944	The S-acylation sites might be involved in stabilizing the transmembrane assembly of E1 and E2.
35149682	0	43	from	resolution	44:53	arg1	Structure					0:8	Structure	0:8	Structure of infective Getah virus at 2.8 Å resolution	0:53	Structure of infective Getah virus at 2.8 Å resolution determined by cryo-electron microscopy.
35149682	10	44	theme	screening	1362:1370	arg1	design					1373:1378	structure-based antiviral and vaccine screening, design, and optimization	1324:1396	design	1373:1378	The structural information will assist structure-based antiviral and vaccine screening, design, and optimization.
35149682	4	45	theme	GETV	626:629	arg1	structure					603:611	the structure	599:611	the structure of infective GETV	599:629	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	8	46	theme	transmembrane	1093:1105	arg1	pocket					1119:1124	a transmembrane hydrophobic pocket	1091:1124	a transmembrane hydrophobic pocket	1091:1124	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	9	47	theme	envelope	1266:1273	arg1	membrane					1275:1282	the viral envelope membrane	1256:1282	the viral envelope membrane	1256:1282	The cholesterol and phospholipid stabilize the hydrophobic pocket in the viral envelope membrane.
35149682	5	48	gly	glycosylation	771:783	arg2	sites					801:805	numerous glycosylation and S-acylation sites	762:805	numerous glycosylation and S-acylation sites in E1 and E2	762:818	We have identified numerous glycosylation and S-acylation sites in E1 and E2.
35149682	4	49	theme	envelope	709:716	arg1	E2					739:740	E2	739:740	E2	739:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	49	theme	envelope	709:716	arg1	E1					732:733	E1	732:733	E1	732:733	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	49	theme	envelope	709:716	arg1	glycoproteins					718:730	the envelope glycoproteins	705:730	the envelope glycoproteins E1 and E2	705:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	50	theme	infective	616:624	arg1	GETV					626:629	infective GETV	616:629	infective GETV	616:629	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	51	theme	capsid	686:691	arg1	protein					693:699	the capsid protein	682:699	the capsid protein	682:699	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	10	52	theme	structural	1289:1298	arg1	information					1300:1310	The structural information	1285:1310	The structural information	1285:1310	The structural information will assist structure-based antiviral and vaccine screening, design, and optimization.
35149682	4	53	theme	Å	654:654	arg1	E2					739:740	E2	739:740	E2	739:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	53	theme	Å	654:654	arg1	resolution					636:645	a resolution	634:645	a resolution of 2.8 Å with the atomic models of the capsid protein	634:699	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	53	theme	Å	654:654	arg1	E1					732:733	E1	732:733	E1	732:733	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	53	theme	Å	654:654	arg1	glycoproteins					718:730	the envelope glycoproteins	705:730	the envelope glycoproteins E1 and E2	705:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	3	54	theme	structure	552:560	arg1	knowledge					535:543	knowledge	535:543	knowledge of the structure of the GETV virion	535:579	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	3	55	theme	GETV	422:425	arg1	unknown					439:445	unknown	439:445	unknown	439:445	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	3	55	theme	GETV	422:425	arg1	properties					408:417	The biological and pathological properties	376:417	The biological and pathological properties of GETV	376:425	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	6	56	theme	cell	909:912	arg1	invasion					914:921	host cell invasion	904:921	host cell invasion	904:921	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	3	57	theme	knowledge	535:543	arg1	lack					527:530	a lack	525:530	a lack of knowledge of the structure of the GETV virion	525:579	The biological and pathological properties of GETV are largely unknown and antiviral or vaccine treatments against GETV are still unavailable due to a lack of knowledge of the structure of the GETV virion.
35149682	4	58	dep	glycoproteins	718:730	arg1	E2					739:740	E2	739:740	E2	739:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	58	dep	glycoproteins	718:730	arg1	E1					732:733	E1	732:733	E1	732:733	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	58	dep	glycoproteins	718:730	arg1	glycoproteins					718:730	the envelope glycoproteins	705:730	the envelope glycoproteins E1 and E2	705:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	5	59	theme	glycosylation	771:783	arg1	sites					801:805	numerous glycosylation and S-acylation sites	762:805	numerous glycosylation and S-acylation sites in E1 and E2	762:818	We have identified numerous glycosylation and S-acylation sites in E1 and E2.
35149682	6	60	theme	host	904:907	arg1	invasion					914:921	host cell invasion	904:921	host cell invasion	904:921	The surface-exposed glycans indicate a possible impact on viral immune evasion and host cell invasion.
35149682	8	61	theme	hydrophobic	1107:1117	arg1	pocket					1119:1124	a transmembrane hydrophobic pocket	1091:1124	a transmembrane hydrophobic pocket	1091:1124	In addition, a cholesterol and a phospholipid molecule are observed in a transmembrane hydrophobic pocket, together with two more cholesterols surrounding the pocket.
35149682	4	62	gly	glycoproteins	718:730	arg1	E2					739:740	E2	739:740	E2	739:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	62	gly	glycoproteins	718:730	arg1	E1					732:733	E1	732:733	E1	732:733	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	4	62	gly	glycoproteins	718:730	arg1	glycoproteins					718:730	the envelope glycoproteins	705:730	the envelope glycoproteins E1 and E2	705:740	Here, we present the structure of infective GETV at a resolution of 2.8 Å with the atomic models of the capsid protein and the envelope glycoproteins E1 and E2.
35149682	2	63	theme	people	304:309	arg1	%					291:291	over 10%	284:291	over 10% of healthy people	284:309	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	2	63	theme	people	304:309	arg1	people					304:309	healthy people	296:309	healthy people	296:309	Although antibodies to GETV have been found in over 10% of healthy people, there are no reports of clinical symptoms associated with GETV.
35149682	9	64	theme	viral	1260:1264	arg1	membrane					1275:1282	the viral envelope membrane	1256:1282	the viral envelope membrane	1256:1282	The cholesterol and phospholipid stabilize the hydrophobic pocket in the viral envelope membrane.
36200778	17	0	theme	stress	2639:2644	arg1	resistance					2646:2655	stress resistance	2639:2655	stress resistance	2639:2655	This phenomenon is of great interest as prophage-induced MVs could potentially influence bacterial behavior, stress resistance, and vesicle functions.
36200778	9	1	theme	spontaneous	1383:1393	arg1	induction					1419:1427	the spontaneous and mitomycin-triggered induction	1379:1427	the spontaneous and mitomycin-triggered induction of the prophage PLE2	1379:1448	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	8	2	theme	protein	1145:1151	arg1	composition					1153:1163	The protein composition	1141:1163	The protein composition of the vesicles released in the supernatant	1141:1207	The protein composition of the vesicles released in the supernatant was identified and a significant number of prophage proteins was detected.
36200778	11	3	theme	key	1631:1633	arg1	role					1635:1638	a key role	1629:1638	a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress	1629:1748	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	2	4	theme	vesicle	285:291	arg1	formation					293:301	vesicle formation	285:301	vesicle formation	285:301	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	13	5	theme	bacteriophage	2086:2098	arg1	resistance					2100:2109	bacteriophage resistance	2086:2109	bacteriophage resistance	2086:2109	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	17	6	theme	vesicle	2662:2668	arg1	functions					2670:2678	vesicle functions	2662:2678	vesicle functions	2662:2678	This phenomenon is of great interest as prophage-induced MVs could potentially influence bacterial behavior, stress resistance, and vesicle functions.
36200778	4	7	theme	vesicles	648:655	arg1	production					634:643	the production	630:643	the production of vesicles by the lactobacilli family	630:682	To date, no information on the production of vesicles by the lactobacilli family has been reported.
36200778	5	8	theme	vesicle	860:866	arg1	formation					868:876	vesicle formation	860:876	vesicle formation	860:876	Here, we aimed to characterize the MVs released by the Gram-positive bacteria Lacticaseibacillus casei BL23 and also investigated the mechanisms involved in vesicle formation.
36200778	8	9	theme	vesicles	1172:1179	arg1	composition					1153:1163	The protein composition	1141:1163	The protein composition of the vesicles released in the supernatant	1141:1207	The protein composition of the vesicles released in the supernatant was identified and a significant number of prophage proteins was detected.
36200778	16	10	theme	key	2385:2387	arg1	role					2389:2392	a key role	2383:2392	a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23	2383:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	13	11	from	microbiology	1975:1986	arg1	crossroads					1928:1937	the crossroads	1924:1937	the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis	1924:2127	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	9	12	theme	prophage	1436:1443	arg1	PLE2					1445:1448	the prophage PLE2	1432:1448	the prophage PLE2	1432:1448	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	6	13	theme	casei	957:961	arg1	vesicles					968:975	L. casei BL23 vesicles	954:975	L. casei BL23 vesicles	954:975	Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm.
36200778	17	14	theme	prophage-induced	2570:2585	arg1	MVs					2587:2589	prophage-induced MVs	2570:2589	prophage-induced MVs	2570:2589	This phenomenon is of great interest as prophage-induced MVs could potentially influence bacterial behavior, stress resistance, and vesicle functions.
36200778	8	15	theme	prophage	1252:1259	arg1	proteins					1261:1268	prophage proteins	1252:1268	prophage proteins	1252:1268	The protein composition of the vesicles released in the supernatant was identified and a significant number of prophage proteins was detected.
36200778	10	16	theme	prophages	1551:1559	arg1	influence					1538:1546	the influence	1534:1546	the influence of prophages on the membrane integrity of bacteria	1534:1597	Finally, we also demonstrated the influence of prophages on the membrane integrity of bacteria.
36200778	12	17	theme	wide	1868:1871	arg1	variety					1873:1879	a wide variety	1866:1879	a wide variety of functions	1866:1892	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	12	17	theme	wide	1868:1871	arg1	functions					1884:1892	functions	1884:1892	functions	1884:1892	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	10	18	theme	bacteria	1590:1597	arg1	integrity					1577:1585	the membrane integrity	1564:1585	the membrane integrity of bacteria	1564:1597	Finally, we also demonstrated the influence of prophages on the membrane integrity of bacteria.
36200778	15	19	theme	biogenesis	2283:2292	arg1	element					2303:2309	a key element	2297:2309	a key element for promoting and improving their release	2297:2351	Thus, the study of vesicle biogenesis is a key element for promoting and improving their release.
36200778	15	19	theme	biogenesis	2283:2292	arg1	study					2266:2270	the study	2262:2270	the study of vesicle biogenesis	2262:2292	Thus, the study of vesicle biogenesis is a key element for promoting and improving their release.
36200778	13	20	theme	current	1967:1973	arg1	microbiology					1975:1986	current microbiology	1967:1986	current microbiology	1967:1986	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	11	21	theme	stress	1743:1748	arg1	presence					1721:1728	presence	1721:1728	presence	1721:1728	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	11	21	theme	stress	1743:1748	arg1	absence					1710:1716	absence	1710:1716	absence	1710:1716	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	3	22	theme	growth	584:589	arg1	conditions					591:600	growth conditions	584:600	growth conditions	584:600	The impact of these mechanisms on vesicle formation is largely dependent on the strain and growth conditions.
36200778	11	23	theme	L.	1689:1690	arg1	BL23					1698:1701	L. casei BL23	1689:1701	L. casei BL23	1689:1701	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	13	24	theme	gene	2030:2033	arg1	transfer					2035:2042	horizontal gene transfer	2019:2042	horizontal gene transfer	2019:2042	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	15	25	theme	key	2299:2301	arg1	element					2303:2309	a key element	2297:2309	a key element for promoting and improving their release	2297:2351	Thus, the study of vesicle biogenesis is a key element for promoting and improving their release.
36200778	15	25	theme	key	2299:2301	arg1	study					2266:2270	the study	2262:2270	the study of vesicle biogenesis	2262:2292	Thus, the study of vesicle biogenesis is a key element for promoting and improving their release.
36200778	17	26	theme	great	2552:2556	arg1	interest					2558:2565	great interest	2552:2565	great interest	2552:2565	This phenomenon is of great interest as prophage-induced MVs could potentially influence bacterial behavior, stress resistance, and vesicle functions.
36200778	11	27	theme	MVs	1682:1684	arg1	production					1668:1677	the production	1664:1677	the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress	1664:1748	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	0	28	theme	Membrane	64:71	arg1	Vesicles					73:80	Membrane Vesicles	64:80	Membrane Vesicles	64:80	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	1	29	theme	membrane	161:168	arg1	MVs					180:182	MVs	180:182	MVs	180:182	The formation of membrane vesicles (MVs) by Gram-positive bacteria has gained increasing attention over the last decade.
36200778	1	29	theme	membrane	161:168	arg1	vesicles					170:177	membrane vesicles	161:177	membrane vesicles (MVs)	161:183	The formation of membrane vesicles (MVs) by Gram-positive bacteria has gained increasing attention over the last decade.
36200778	11	30	from	production	1668:1677	arg1	presence					1721:1728	presence	1721:1728	presence	1721:1728	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	11	30	from	production	1668:1677	arg1	absence					1710:1716	absence	1710:1716	absence	1710:1716	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	13	31	theme	antibiotic	1996:2005	arg1	resistance					2007:2016	antibiotic resistance	1996:2016	antibiotic resistance	1996:2016	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	3	32	dep	strain	573:578	arg1	the					569:571	the	569:571	the	569:571	The impact of these mechanisms on vesicle formation is largely dependent on the strain and growth conditions.
36200778	0	33	theme	Gram-Positive	89:101	arg1	Bacterium					103:111	the Gram-Positive Bacterium Lacticaseibacillus casei BL23	85:141	the Gram-Positive Bacterium Lacticaseibacillus casei BL23	85:141	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	13	34	theme	topics	1957:1962	arg1	crossroads					1928:1937	the crossroads	1924:1937	the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis	1924:2127	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	6	35	theme	vesicles	968:975	arg1	majority					942:949	the majority	938:949	the majority of L. casei BL23 vesicles	938:975	Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm.
36200778	2	36	theme	peptidoglycan	403:415	arg1	hydrolases					422:431	prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	368:431	prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	368:431	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	9	37	theme	mitomycin-triggered	1399:1417	arg1	induction					1419:1427	the spontaneous and mitomycin-triggered induction	1379:1427	the spontaneous and mitomycin-triggered induction of the prophage PLE2	1379:1448	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	9	38	theme	mutant	1302:1307	arg1	strain					1309:1314	a mutant strain	1300:1314	a mutant strain harboring a defective PLE2 prophage	1300:1350	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	0	39	theme	Spontaneous	0:10	arg1	Induction					21:29	Spontaneous Prophage Induction	0:29	Spontaneous Prophage Induction	0:29	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	12	40	dep	IMPORTANCE	1751:1760	arg1	decades					1775:1781	The last few decades	1762:1781	IMPORTANCE The last few decades	1751:1781	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	9	41	theme	defective	1328:1336	arg1	prophage					1343:1350	a defective PLE2 prophage	1326:1350	a defective PLE2 prophage	1326:1350	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	16	42	theme	spontaneous	2397:2407	arg1	induction					2442:2450	spontaneous and mitomycin-triggered prophage induction	2397:2450	spontaneous and mitomycin-triggered prophage induction	2397:2450	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	2	43	theme	β-lactam	471:478	arg1	antibiotics					480:490	β-lactam antibiotics	471:490	β-lactam antibiotics	471:490	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	7	44	from	growth	1086:1091	arg1	conditions					1129:1138	standard culture conditions	1112:1138	standard culture conditions	1112:1138	Furthermore, we showed that the vesicles were released consistently throughout the growth of the bacteria in standard culture conditions.
36200778	11	45	theme	PLE2	1656:1659	arg1	role					1635:1638	a key role	1629:1638	a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress	1629:1748	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	5	46	theme	Gram-positive	758:770	arg1	bacteria					772:779	Gram-positive bacteria	758:779	the Gram-positive bacteria Lacticaseibacillus casei BL23	754:809	Here, we aimed to characterize the MVs released by the Gram-positive bacteria Lacticaseibacillus casei BL23 and also investigated the mechanisms involved in vesicle formation.
36200778	12	47	theme	membrane	1806:1813	arg1	MVs					1825:1827	MVs	1825:1827	MVs	1825:1827	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	12	47	theme	membrane	1806:1813	arg1	vesicles					1815:1822	membrane vesicles	1806:1822	membrane vesicles (MVs) produced by microorganisms	1806:1855	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	16	48	theme	mitomycin-triggered	2413:2431	arg1	induction					2442:2450	spontaneous and mitomycin-triggered prophage induction	2397:2450	spontaneous and mitomycin-triggered prophage induction	2397:2450	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	2	49	theme	synthesis	458:466	arg1	digestion					338:346	the digestion	334:346	the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	334:431	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	2	49	theme	synthesis	458:466	arg1	inhibition					441:450	the inhibition	437:450	the inhibition of PG synthesis by β-lactam antibiotics	437:490	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	15	50	theme	vesicle	2275:2281	arg1	biogenesis					2283:2292	vesicle biogenesis	2275:2292	vesicle biogenesis	2275:2292	Thus, the study of vesicle biogenesis is a key element for promoting and improving their release.
36200778	5	51	theme	Lacticaseibacillus	781:798	arg1	BL23					806:809	the Gram-positive bacteria Lacticaseibacillus casei BL23	754:809	the Gram-positive bacteria Lacticaseibacillus casei BL23	754:809	Here, we aimed to characterize the MVs released by the Gram-positive bacteria Lacticaseibacillus casei BL23 and also investigated the mechanisms involved in vesicle formation.
36200778	12	52	theme	last	1766:1769	arg1	decades					1775:1781	The last few decades	1762:1781	IMPORTANCE The last few decades	1751:1781	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	16	53	theme	induction	2442:2450	arg1	role					2389:2392	a key role	2383:2392	a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23	2383:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	13	54	theme	research	1948:1955	arg1	topics					1957:1962	major research topics	1942:1962	major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis	1942:2127	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	54	theme	research	1948:1955	arg1	communication					2050:2062	cell communication	2045:2062	cell communication	2045:2062	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	54	theme	research	1948:1955	arg1	resistance					2100:2109	bacteriophage resistance	2086:2109	bacteriophage resistance	2086:2109	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	54	theme	research	1948:1955	arg1	pathogenesis					2116:2127	pathogenesis	2116:2127	pathogenesis	2116:2127	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	54	theme	research	1948:1955	arg1	transfer					2035:2042	horizontal gene transfer	2019:2042	horizontal gene transfer	2019:2042	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	54	theme	research	1948:1955	arg1	development					2073:2083	biofilm development	2065:2083	biofilm development	2065:2083	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	54	theme	research	1948:1955	arg1	resistance					2007:2016	antibiotic resistance	1996:2016	antibiotic resistance	1996:2016	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	16	55	theme	MV	2455:2456	arg1	production					2458:2467	MV production	2455:2467	MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23	2455:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	14	56	theme	probiotic	2166:2174	arg1	strains					2176:2182	probiotic strains	2166:2182	probiotic strains	2166:2182	In particular, vesicles produced by probiotic strains have been shown to play a significant role in their beneficial effects.
36200778	3	57	theme	mechanisms	513:522	arg1	impact					497:502	The impact	493:502	The impact of these mechanisms on vesicle formation	493:543	The impact of these mechanisms on vesicle formation is largely dependent on the strain and growth conditions.
36200778	3	57	theme	mechanisms	513:522	arg1	dependent					556:564	dependent	556:564	dependent	556:564	The impact of these mechanisms on vesicle formation is largely dependent on the strain and growth conditions.
36200778	7	58	theme	standard	1112:1119	arg1	conditions					1129:1138	standard culture conditions	1112:1138	standard culture conditions	1112:1138	Furthermore, we showed that the vesicles were released consistently throughout the growth of the bacteria in standard culture conditions.
36200778	7	59	theme	bacteria	1100:1107	arg1	growth					1086:1091	the growth	1082:1091	the growth of the bacteria in standard culture conditions	1082:1138	Furthermore, we showed that the vesicles were released consistently throughout the growth of the bacteria in standard culture conditions.
36200778	2	60	theme	cell	355:358	arg1	wall					360:363	the cell wall	351:363	the cell wall	351:363	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	13	61	theme	biofilm	2065:2071	arg1	development					2073:2083	biofilm development	2065:2083	biofilm development	2065:2083	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	2	62	theme	formation	293:301	arg1	models					275:280	models	275:280	models of vesicle formation	275:301	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	3	63	theme	vesicle	527:533	arg1	formation					535:543	vesicle formation	527:543	vesicle formation	527:543	The impact of these mechanisms on vesicle formation is largely dependent on the strain and growth conditions.
36200778	7	64	theme	culture	1121:1127	arg1	conditions					1129:1138	standard culture conditions	1112:1138	standard culture conditions	1112:1138	Furthermore, we showed that the vesicles were released consistently throughout the growth of the bacteria in standard culture conditions.
36200778	6	65	theme	electron	885:892	arg1	microscopy					894:903	electron microscopy	885:903	electron microscopy	885:903	Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm.
36200778	10	66	theme	membrane	1568:1575	arg1	integrity					1577:1585	the membrane integrity	1564:1585	the membrane integrity of bacteria	1564:1597	Finally, we also demonstrated the influence of prophages on the membrane integrity of bacteria.
36200778	12	67	theme	functions	1884:1892	arg1	variety					1873:1879	a wide variety	1866:1879	a wide variety of functions	1866:1892	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	12	67	theme	functions	1884:1892	arg1	functions					1884:1892	functions	1884:1892	functions	1884:1892	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	6	68	theme	BL23	963:966	arg1	vesicles					968:975	L. casei BL23 vesicles	954:975	L. casei BL23 vesicles	954:975	Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm.
36200778	8	69	theme	significant	1230:1240	arg1	number					1242:1247	a significant number	1228:1247	a significant number of prophage proteins	1228:1268	The protein composition of the vesicles released in the supernatant was identified and a significant number of prophage proteins was detected.
36200778	13	70	from	topics	1957:1962	arg1	microbiology					1975:1986	current microbiology	1967:1986	current microbiology	1967:1986	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	11	71	theme	genotoxic	1733:1741	arg1	stress					1743:1748	genotoxic stress	1733:1748	genotoxic stress	1733:1748	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	3	72	from	impact	497:502	arg1	formation					535:543	vesicle formation	527:543	vesicle formation	527:543	The impact of these mechanisms on vesicle formation is largely dependent on the strain and growth conditions.
36200778	9	73	theme	PLE2	1445:1448	arg1	induction					1419:1427	the spontaneous and mitomycin-triggered induction	1379:1427	the spontaneous and mitomycin-triggered induction of the prophage PLE2	1379:1448	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	6	74	theme	L.	954:955	arg1	vesicles					968:975	L. casei BL23 vesicles	954:975	L. casei BL23 vesicles	954:975	Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm.
36200778	13	75	theme	cell	2045:2048	arg1	communication					2050:2062	cell communication	2045:2062	cell communication	2045:2062	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	4	76	from	information	615:625	arg1	production					634:643	the production	630:643	the production of vesicles by the lactobacilli family	630:682	To date, no information on the production of vesicles by the lactobacilli family has been reported.
36200778	13	77	from	crossroads	1928:1937	arg1	microbiology					1975:1986	current microbiology	1967:1986	current microbiology	1967:1986	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	6	78	theme	majority	942:949	arg1	size					930:933	the size	926:933	the size of the majority of L. casei BL23 vesicles	926:975	Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm.
36200778	8	79	theme	proteins	1261:1268	arg1	number					1242:1247	a significant number	1228:1247	a significant number of prophage proteins	1228:1268	The protein composition of the vesicles released in the supernatant was identified and a significant number of prophage proteins was detected.
36200778	17	80	theme	bacterial	2619:2627	arg1	behavior					2629:2636	bacterial behavior	2619:2636	bacterial behavior	2619:2636	This phenomenon is of great interest as prophage-induced MVs could potentially influence bacterial behavior, stress resistance, and vesicle functions.
36200778	13	81	theme	horizontal	2019:2028	arg1	transfer					2035:2042	horizontal gene transfer	2019:2042	horizontal gene transfer	2019:2042	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	9	82	theme	MVs	1482:1484	arg1	production					1468:1477	the production	1464:1477	the production of MVs by L. casei BL23	1464:1501	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	11	83	theme	casei	1692:1696	arg1	BL23					1698:1701	L. casei BL23	1689:1701	L. casei BL23	1689:1701	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	0	84	theme	Vesicles	73:80	arg1	Production					50:59	the Production	46:59	the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23	46:141	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	11	85	dep	absence	1710:1716	arg1	the					1706:1708	the	1706:1708	the	1706:1708	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	9	86	theme	L.	1489:1490	arg1	casei					1492:1496	L. casei BL23	1489:1501	L. casei BL23	1489:1501	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	16	87	theme	Gram-positive	2476:2488	arg1	bacteria					2490:2497	the Gram-positive bacteria Lacticaseibacillus casei BL23	2472:2527	the Gram-positive bacteria Lacticaseibacillus casei BL23	2472:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	4	88	theme	lactobacilli	664:675	arg1	family					677:682	the lactobacilli family	660:682	the lactobacilli family	660:682	To date, no information on the production of vesicles by the lactobacilli family has been reported.
36200778	14	89	theme	beneficial	2236:2245	arg1	effects					2247:2253	their beneficial effects	2230:2253	their beneficial effects	2230:2253	In particular, vesicles produced by probiotic strains have been shown to play a significant role in their beneficial effects.
36200778	1	90	theme	vesicles	170:177	arg1	formation					148:156	The formation	144:156	The formation of membrane vesicles (MVs) by Gram-positive bacteria	144:209	The formation of membrane vesicles (MVs) by Gram-positive bacteria has gained increasing attention over the last decade.
36200778	16	91	theme	Lacticaseibacillus	2499:2516	arg1	BL23					2524:2527	Lacticaseibacillus casei BL23	2499:2527	the Gram-positive bacteria Lacticaseibacillus casei BL23	2472:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	6	92	dep	100 nm	995:1000	arg1	to					992:993	to	992:993	to	992:993	Using electron microscopy, we established that the size of the majority of L. casei BL23 vesicles ranged from 50 to 100 nm.
36200778	14	93	theme	significant	2210:2220	arg1	role					2222:2225	a significant role	2208:2225	a significant role	2208:2225	In particular, vesicles produced by probiotic strains have been shown to play a significant role in their beneficial effects.
36200778	0	94	theme	casei	132:136	arg1	BL23					138:141	casei BL23	132:141	the Gram-Positive Bacterium Lacticaseibacillus casei BL23	85:141	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	0	95	dep	Bacterium	103:111	arg1	BL23					138:141	casei BL23	132:141	the Gram-Positive Bacterium Lacticaseibacillus casei BL23	85:141	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	0	95	dep	Bacterium	103:111	arg1	Lacticaseibacillus					113:130	the Gram-Positive Bacterium Lacticaseibacillus casei BL23	85:141	the Gram-Positive Bacterium Lacticaseibacillus casei BL23	85:141	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	16	96	theme	casei	2518:2522	arg1	BL23					2524:2527	Lacticaseibacillus casei BL23	2499:2527	the Gram-positive bacteria Lacticaseibacillus casei BL23	2472:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	2	97	theme	stress-induced	388:401	arg1	hydrolases					422:431	prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	368:431	prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	368:431	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	5	98	theme	casei	800:804	arg1	BL23					806:809	the Gram-positive bacteria Lacticaseibacillus casei BL23	754:809	the Gram-positive bacteria Lacticaseibacillus casei BL23	754:809	Here, we aimed to characterize the MVs released by the Gram-positive bacteria Lacticaseibacillus casei BL23 and also investigated the mechanisms involved in vesicle formation.
36200778	2	99	theme	prophage-encoded	368:383	arg1	hydrolases					422:431	prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	368:431	prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	368:431	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	11	100	from	role	1635:1638	arg1	production					1668:1677	the production	1664:1677	the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress	1664:1748	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	11	101	theme	prophage	1647:1654	arg1	PLE2					1656:1659	the prophage PLE2	1643:1659	the prophage PLE2	1643:1659	Overall, our results suggest a key role of the prophage PLE2 in the production of MVs by L. casei BL23 in the absence or presence of genotoxic stress.
36200778	12	102	contain	have	1861:1864	arg1	MVs					1825:1827	MVs	1825:1827	MVs	1825:1827	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	12	102	contain	have	1861:1864	arg2	variety					1873:1879	a wide variety	1866:1879	a wide variety of functions	1866:1892	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	12	102	contain	have	1861:1864	arg2	functions					1884:1892	functions	1884:1892	functions	1884:1892	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	12	102	contain	have	1861:1864	arg1	vesicles					1815:1822	membrane vesicles	1806:1822	membrane vesicles (MVs) produced by microorganisms	1806:1855	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	16	103	from	role	2389:2392	arg1	production					2458:2467	MV production	2455:2467	MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23	2455:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	9	104	theme	PLE2	1338:1341	arg1	prophage					1343:1350	a defective PLE2 prophage	1326:1350	a defective PLE2 prophage	1326:1350	Moreover, using a mutant strain harboring a defective PLE2 prophage, we were able to show that the spontaneous and mitomycin-triggered induction of the prophage PLE2 contribute to the production of MVs by L. casei BL23.
36200778	5	105	theme	bacteria	772:779	arg1	BL23					806:809	the Gram-positive bacteria Lacticaseibacillus casei BL23	754:809	the Gram-positive bacteria Lacticaseibacillus casei BL23	754:809	Here, we aimed to characterize the MVs released by the Gram-positive bacteria Lacticaseibacillus casei BL23 and also investigated the mechanisms involved in vesicle formation.
36200778	12	106	theme	few	1771:1773	arg1	decades					1775:1781	The last few decades	1762:1781	IMPORTANCE The last few decades	1751:1781	IMPORTANCE The last few decades have demonstrated that membrane vesicles (MVs) produced by microorganisms can have a wide variety of functions.
36200778	1	107	theme	Gram-positive	188:200	arg1	bacteria					202:209	Gram-positive bacteria	188:209	Gram-positive bacteria	188:209	The formation of membrane vesicles (MVs) by Gram-positive bacteria has gained increasing attention over the last decade.
36200778	2	108	theme	PG	455:456	arg1	synthesis					458:466	PG synthesis	455:466	PG synthesis	455:466	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	13	109	theme	major	1942:1946	arg1	topics					1957:1962	major research topics	1942:1962	major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis	1942:2127	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	109	theme	major	1942:1946	arg1	communication					2050:2062	cell communication	2045:2062	cell communication	2045:2062	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	109	theme	major	1942:1946	arg1	resistance					2100:2109	bacteriophage resistance	2086:2109	bacteriophage resistance	2086:2109	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	109	theme	major	1942:1946	arg1	pathogenesis					2116:2127	pathogenesis	2116:2127	pathogenesis	2116:2127	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	109	theme	major	1942:1946	arg1	transfer					2035:2042	horizontal gene transfer	2019:2042	horizontal gene transfer	2019:2042	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	109	theme	major	1942:1946	arg1	development					2073:2083	biofilm development	2065:2083	biofilm development	2065:2083	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	13	109	theme	major	1942:1946	arg1	resistance					2007:2016	antibiotic resistance	1996:2016	antibiotic resistance	1996:2016	This diversity places MVs at the crossroads of major research topics in current microbiology such as antibiotic resistance, horizontal gene transfer, cell communication, biofilm development, bacteriophage resistance, and pathogenesis.
36200778	16	110	theme	prophage	2433:2440	arg1	induction					2442:2450	spontaneous and mitomycin-triggered prophage induction	2397:2450	spontaneous and mitomycin-triggered prophage induction	2397:2450	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	2	111	theme	wall	360:363	arg1	digestion					338:346	the digestion	334:346	the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases	334:431	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	2	111	theme	wall	360:363	arg1	inhibition					441:450	the inhibition	437:450	the inhibition of PG synthesis by β-lactam antibiotics	437:490	Recently, models of vesicle formation have been proposed and involve the digestion of the cell wall by prophage-encoded or stress-induced peptidoglycan (PG) hydrolases and the inhibition of PG synthesis by β-lactam antibiotics.
36200778	10	112	from	influence	1538:1546	arg1	integrity					1577:1585	the membrane integrity	1564:1585	the membrane integrity of bacteria	1564:1597	Finally, we also demonstrated the influence of prophages on the membrane integrity of bacteria.
36200778	16	113	dep	bacteria	2490:2497	arg1	BL23					2524:2527	Lacticaseibacillus casei BL23	2499:2527	the Gram-positive bacteria Lacticaseibacillus casei BL23	2472:2527	Overall, our results suggest a key role of spontaneous and mitomycin-triggered prophage induction in MV production by the Gram-positive bacteria Lacticaseibacillus casei BL23.
36200778	0	114	theme	Prophage	12:19	arg1	Induction					21:29	Spontaneous Prophage Induction	0:29	Spontaneous Prophage Induction	0:29	Spontaneous Prophage Induction Contributes to the Production of Membrane Vesicles by the Gram-Positive Bacterium Lacticaseibacillus casei BL23.
36200778	1	115	theme	last	252:255	arg1	decade					257:262	the last decade	248:262	the last decade	248:262	The formation of membrane vesicles (MVs) by Gram-positive bacteria has gained increasing attention over the last decade.
35074011	0	0	theme	cell	88:91	arg1	generation					93:102	IL-35+ B cell generation	79:102	IL-35+ B cell generation	79:102	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	12	1	dep	Abstract	1549:1556	arg1	Video					1543:1547	Video	1543:1547	Video	1543:1547	Video Abstract.
35074011	10	2	theme	IL-35+	1432:1437	arg1	cells					1441:1445	IL-35+ B cells	1432:1445	IL-35+ B cells	1432:1445	KO of PXR or TLR4 impaired the generation of IL-35+ B cells.
35074011	1	3	theme	chronic	173:179	arg1	illnesses					181:189	chronic illnesses	173:189	chronic illnesses worldwide	173:199	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	0	4	theme	B	86:86	arg1	generation					93:102	IL-35+ B cell generation	79:102	IL-35+ B cell generation	79:102	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	5	5	theme	gut	695:697	arg1	Reg4					699:702	gut Reg4	695:702	gut Reg4 associated with bacteria such as Lactobacillus	695:749	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	5	6	theme	liquid	629:634	arg1	LC-MS					622:626	LC-MS	622:626	LC-MS (liquid chromatography-mass spectrometry)/MS	622:671	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	5	6	theme	liquid	629:634	arg1	spectrometry					656:667	liquid chromatography-mass spectrometry	629:667	liquid chromatography-mass spectrometry	629:667	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	1	7	theme	illnesses	181:189	arg1	worldwide					191:199	chronic illnesses worldwide	173:199	chronic illnesses worldwide	173:199	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	4	8	dep	RESULTS	491:497	arg1	We					499:500	We	499:500	We	499:500	RESULTS We first found that gut Reg4 promoted resistance to high-fat diet-induced obesity.
35074011	8	9	located	detected	1208:1215	arg2	levels					1184:1189	Lower levels	1178:1189	Lower levels of IAA	1178:1196	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	8	9	located	detected	1208:1215	arg1	blood					1235:1239	the peripheral blood	1220:1239	the peripheral blood of individuals with obesity	1220:1267	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	5	10	theme	chromatography-mass	636:654	arg1	LC-MS					622:626	LC-MS	622:626	LC-MS (liquid chromatography-mass spectrometry)/MS	622:671	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	5	10	theme	chromatography-mass	636:654	arg1	spectrometry					656:667	liquid chromatography-mass spectrometry	629:667	liquid chromatography-mass spectrometry	629:667	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	2	11	theme	IL-35+	322:327	arg1	cells					329:333	these IL-35+ cells	316:333	these IL-35+ cells	316:333	However, whether the gut microbiota regulates these IL-35+ cells remains elusive.
35074011	6	12	theme	colon	1061:1065	arg1	tissues					1067:1073	the adipose and colon tissues	1045:1073	tissues	1067:1073	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	6	13	theme	cell	1011:1014	arg1	accumulation					1016:1027	decreased IL-35+ cell accumulation	994:1027	decreased IL-35+ cell accumulation	994:1027	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	5	14	theme	IL-35+	778:783	arg1	cells					787:791	IL-35+ B cells	778:791	IL-35+ B cells	778:791	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	0	15	link	microbiota-derived	4:21	arg1	acid					48:51	Gut microbiota-derived metabolite 3-idoleacetic acid	0:51	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS	0:69	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	6	16	theme	mice	1097:1100	arg1	tissues					1067:1073	the adipose and colon tissues	1045:1073	tissues	1067:1073	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	6	17	theme	IL-35+	1004:1009	arg1	accumulation					1016:1027	decreased IL-35+ cell accumulation	994:1027	decreased IL-35+ cell accumulation	994:1027	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	3	18	theme	regulatory	379:388	arg1	effects					390:396	the regulatory effects	375:396	the regulatory effects of the gut microbiota on IL-35+ cells	375:434	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	8	19	theme	Lower	1178:1182	arg1	levels					1184:1189	Lower levels	1178:1189	Lower levels of IAA	1178:1196	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	9	20	theme	B	1357:1357	arg1	cells					1359:1363	IL-35+ B cells	1350:1363	IL-35+ B cells	1350:1363	Mechanistically, IAA together with LPS mediated IL-35+ B cells through PXR and TLR4.
35074011	6	21	theme	knockout	1083:1090	arg1	mice					1097:1100	Reg4 knockout (KO) mice	1078:1100	Reg4 knockout (KO) mice	1078:1100	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	6	22	theme	KO	1093:1094	arg1	mice					1097:1100	Reg4 knockout (KO) mice	1078:1100	Reg4 knockout (KO) mice	1078:1100	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	5	23	theme	cells	787:791	arg1	generation					764:773	the generation	760:773	the generation of IL-35+ B cells	760:791	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	4	24	theme	gut	519:521	arg1	Reg4					523:526	gut Reg4	519:526	gut Reg4	519:526	RESULTS We first found that gut Reg4 promoted resistance to high-fat diet-induced obesity.
35074011	3	25	theme	IL-35+	423:428	arg1	cells					430:434	IL-35+ cells	423:434	IL-35+ cells	423:434	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	5	26	theme	B	785:785	arg1	cells					787:791	IL-35+ B cells	778:791	IL-35+ B cells	778:791	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	0	27	theme	microbiota-derived	4:21	arg1	acid					48:51	Gut microbiota-derived metabolite 3-idoleacetic acid	0:51	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS	0:69	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	6	28	theme	IL-35+	904:909	arg1	accumulation					916:927	marked IL-35+ cell accumulation	897:927	marked IL-35+ cell accumulation	897:927	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	10	29	theme	cells	1441:1445	arg1	generation					1418:1427	the generation	1414:1427	the generation of IL-35+ B cells	1414:1445	KO of PXR or TLR4 impaired the generation of IL-35+ B cells.
35074011	10	30	theme	TLR4	1400:1403	arg1	KO					1387:1388	KO	1387:1388	KO of PXR or TLR4	1387:1403	KO of PXR or TLR4 impaired the generation of IL-35+ B cells.
35074011	3	31	theme	modified	457:464	arg1	models					472:477	genetically modified mouse models	445:477	genetically modified mouse models of obesity	445:488	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	0	32	theme	Gut	0:2	arg1	acid					48:51	Gut microbiota-derived metabolite 3-idoleacetic acid	0:51	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS	0:69	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	6	33	theme	decreased	994:1002	arg1	accumulation					1016:1027	decreased IL-35+ cell accumulation	994:1027	decreased IL-35+ cell accumulation	994:1027	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	10	34	theme	B	1439:1439	arg1	cells					1441:1445	IL-35+ B cells	1432:1445	IL-35+ B cells	1432:1445	KO of PXR or TLR4 impaired the generation of IL-35+ B cells.
35074011	5	35	theme	LC-MS	622:626	arg1	/MS					669:671	LC-MS (liquid chromatography-mass spectrometry)/MS	622:671	LC-MS (liquid chromatography-mass spectrometry)/MS	622:671	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	3	36	theme	mouse	466:470	arg1	models					472:477	genetically modified mouse models	445:477	genetically modified mouse models of obesity	445:488	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	0	37	theme	3-idoleacetic	34:46	arg1	acid					48:51	Gut microbiota-derived metabolite 3-idoleacetic acid	0:51	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS	0:69	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	10	38	theme	PXR	1393:1395	arg1	KO					1387:1388	KO	1387:1388	KO of PXR or TLR4	1387:1403	KO of PXR or TLR4 impaired the generation of IL-35+ B cells.
35074011	1	39	theme	BACKGROUND	105:114	arg1	Bregs					132:136	BACKGROUND IL-35-producing Bregs	105:136	BACKGROUND IL-35-producing Bregs	105:136	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	11	40	theme	B	1513:1513	arg1	cells					1515:1519	IL-35+ B cells	1506:1519	IL-35+ B cells	1506:1519	CONCLUSION Together, IAA and LPS induce the generation of IL-35+ B cells through PXR and TLR4.
35074011	3	41	theme	microbiota	409:418	arg1	effects					390:396	the regulatory effects	375:396	the regulatory effects of the gut microbiota on IL-35+ cells	375:434	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	9	42	theme	IL-35+	1350:1355	arg1	cells					1359:1363	IL-35+ B cells	1350:1363	IL-35+ B cells	1350:1363	Mechanistically, IAA together with LPS mediated IL-35+ B cells through PXR and TLR4.
35074011	6	43	theme	marked	897:902	arg1	accumulation					916:927	marked IL-35+ cell accumulation	897:927	marked IL-35+ cell accumulation	897:927	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	1	44	theme	IL-35-producing	116:130	arg1	Bregs					132:136	BACKGROUND IL-35-producing Bregs	105:136	BACKGROUND IL-35-producing Bregs	105:136	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	5	45	theme	LPS	845:847	arg1	presence					833:840	the presence	829:840	the presence of LPS	829:847	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	7	46	theme	obesity	1148:1154	arg1	resistance					1156:1165	HFD-induced obesity resistance	1136:1165	HFD-induced obesity resistance	1136:1165	We also found that Reg4 mediated HFD-induced obesity resistance via IL-35.
35074011	6	47	theme	cell	911:914	arg1	accumulation					916:927	marked IL-35+ cell accumulation	897:927	marked IL-35+ cell accumulation	897:927	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	3	48	from	effects	390:396	arg1	cells					430:434	IL-35+ cells	423:434	IL-35+ cells	423:434	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	1	49	theme	related	216:222	arg1	mechanisms					205:214	mechanisms	205:214	mechanisms related to disrupting the gut microbiota composition	205:267	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	3	50	theme	obesity	482:488	arg1	models					472:477	genetically modified mouse models	445:477	genetically modified mouse models of obesity	445:488	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	4	51	theme	diet-induced	560:571	arg1	obesity					573:579	high-fat diet-induced obesity	551:579	high-fat diet-induced obesity	551:579	RESULTS We first found that gut Reg4 promoted resistance to high-fat diet-induced obesity.
35074011	3	52	theme	gut	405:407	arg1	microbiota					409:418	the gut microbiota	401:418	the gut microbiota	401:418	We herein investigated the regulatory effects of the gut microbiota on IL-35+ cells by using genetically modified mouse models of obesity.
35074011	1	53	theme	gut	242:244	arg1	composition					257:267	the gut microbiota composition	238:267	the gut microbiota composition	238:267	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	4	54	theme	high-fat	551:558	arg1	obesity					573:579	high-fat diet-induced obesity	551:579	high-fat diet-induced obesity	551:579	RESULTS We first found that gut Reg4 promoted resistance to high-fat diet-induced obesity.
35074011	6	55	theme	high-fat	873:880	arg1	diet					882:885	a high-fat diet	871:885	a high-fat diet	871:885	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	5	56	theme	16S	588:590	arg1	sequencing					597:606	16S rRNA sequencing	588:606	16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS	588:671	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	7	57	theme	HFD-induced	1136:1146	arg1	resistance					1156:1165	HFD-induced obesity resistance	1136:1165	HFD-induced obesity resistance	1136:1165	We also found that Reg4 mediated HFD-induced obesity resistance via IL-35.
35074011	8	58	with	individuals	1244:1254	arg1	obesity					1261:1267	obesity	1261:1267	obesity	1261:1267	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	11	59	theme	cells	1515:1519	arg1	generation					1492:1501	the generation	1488:1501	the generation of IL-35+ B cells	1488:1519	CONCLUSION Together, IAA and LPS induce the generation of IL-35+ B cells through PXR and TLR4.
35074011	5	60	theme	rRNA	592:595	arg1	sequencing					597:606	16S rRNA sequencing	588:606	16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS	588:671	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	8	61	theme	nonobese	1283:1290	arg1	subjects					1292:1299	nonobese subjects	1283:1299	nonobese subjects	1283:1299	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	6	62	dep	exhibited	887:895	arg1	whereas					986:992	whereas	986:992	whereas	986:992	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	8	63	theme	IAA	1194:1196	arg1	levels					1184:1189	Lower levels	1178:1189	Lower levels of IAA	1178:1196	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	2	64	theme	gut	291:293	arg1	microbiota					295:304	the gut microbiota	287:304	the gut microbiota	287:304	However, whether the gut microbiota regulates these IL-35+ cells remains elusive.
35074011	8	65	theme	individuals	1244:1254	arg1	blood					1235:1239	the peripheral blood	1220:1239	the peripheral blood of individuals with obesity	1220:1267	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	1	66	theme	Treg	142:145	arg1	cells					147:151	Treg cells	142:151	Treg cells	142:151	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	6	67	located	observed	1033:1040	arg1	tissues					1067:1073	the adipose and colon tissues	1045:1073	tissues	1067:1073	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	6	67	located	observed	1033:1040	arg2	accumulation					1016:1027	decreased IL-35+ cell accumulation	994:1027	decreased IL-35+ cell accumulation	994:1027	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	1	68	theme	microbiota	246:255	arg1	composition					257:267	the gut microbiota composition	238:267	the gut microbiota composition	238:267	BACKGROUND IL-35-producing Bregs and Treg cells critically regulate chronic illnesses worldwide via mechanisms related to disrupting the gut microbiota composition.
35074011	6	69	theme	Reg4	1078:1081	arg1	mice					1097:1100	Reg4 knockout (KO) mice	1078:1100	Reg4 knockout (KO) mice	1078:1100	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	0	70	theme	IL-35+	79:84	arg1	generation					93:102	IL-35+ B cell generation	79:102	IL-35+ B cell generation	79:102	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	6	71	theme	HuREG4IECtg	850:860	arg1	mice					862:865	HuREG4IECtg mice	850:865	HuREG4IECtg mice fed a high-fat diet	850:885	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	8	72	theme	peripheral	1224:1233	arg1	blood					1235:1239	the peripheral blood	1220:1239	the peripheral blood of individuals with obesity	1220:1267	Lower levels of IAA were also detected in the peripheral blood of individuals with obesity compared with nonobese subjects.
35074011	5	73	theme	3-idoleacetic	801:813	arg1	IAA					821:823	IAA	821:823	IAA	821:823	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	5	73	theme	3-idoleacetic	801:813	arg1	acid					815:818	3-idoleacetic acid	801:818	3-idoleacetic acid (IAA)	801:824	Using 16S rRNA sequencing combined with LC-MS (liquid chromatography-mass spectrometry)/MS, we demonstrated that gut Reg4 associated with bacteria such as Lactobacillus promoted the generation of IL-35+ B cells through 3-idoleacetic acid (IAA) in the presence of LPS.
35074011	6	74	theme	adipose	947:953	arg1	tissues					955:961	their adipose tissues	941:961	not only their adipose tissues but also their colons	932:983	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	0	75	theme	metabolite	23:32	arg1	acid					48:51	Gut microbiota-derived metabolite 3-idoleacetic acid	0:51	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS	0:69	Gut microbiota-derived metabolite 3-idoleacetic acid together with LPS induces IL-35+ B cell generation.
35074011	6	76	theme	adipose	1049:1055	arg1	tissues					1067:1073	the adipose and colon tissues	1045:1073	tissues	1067:1073	HuREG4IECtg mice fed a high-fat diet exhibited marked IL-35+ cell accumulation in not only their adipose tissues but also their colons, whereas decreased IL-35+ cell accumulation was observed in the adipose and colon tissues of Reg4 knockout (KO) mice.
35074011	11	77	theme	IL-35+	1506:1511	arg1	cells					1515:1519	IL-35+ B cells	1506:1519	IL-35+ B cells	1506:1519	CONCLUSION Together, IAA and LPS induce the generation of IL-35+ B cells through PXR and TLR4.
36555167	11	0	theme	inflammatory	1708:1719	arg1	diseases					1732:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	8	1	theme	TNF-α	1217:1221	arg1	levels					1232:1237	TNF-α and IL-6 levels	1217:1237	levels	1232:1237	LCS-SeNPs significantly reduced serum and colonic inflammatory cytokines TNF-α and IL-6 levels.
36555167	2	2	theme	new	272:274	arg1	form					276:279	a new form	270:279	a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs))	270:371	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	5	3	theme	uniform	870:876	arg1	particles					888:896	zero-valent and orange-red relatively uniform spherical particles	832:896	zero-valent and orange-red relatively uniform spherical particles	832:896	The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared.
36555167	8	4	theme	IL-6	1227:1230	arg1	levels					1232:1237	TNF-α and IL-6 levels	1217:1237	levels	1232:1237	LCS-SeNPs significantly reduced serum and colonic inflammatory cytokines TNF-α and IL-6 levels.
36555167	2	5	theme	Low	307:309	arg1	weight					321:326	Low molecular weight	307:326	Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)	307:370	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	6	6	theme	Se	959:960	arg1	elements					962:969	C, N, O and Se elements	947:969	C, N, O and Se elements	947:969	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	7	7	dep	injury	1066:1071	arg1	symptoms					1090:1097	symptoms	1090:1097	symptoms	1090:1097	LCS-SeNPs reduced colon injury and inflammation symptoms and improved intestinal barrier dysfunction.
36555167	3	8	theme	spectroscopic	550:562	arg1	measurements					580:591	various spectroscopic and microscopic measurements	542:591	various spectroscopic and microscopic measurements	542:591	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	5	9	theme	zero-valent	832:842	arg1	particles					888:896	zero-valent and orange-red relatively uniform spherical particles	832:896	zero-valent and orange-red relatively uniform spherical particles	832:896	The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared.
36555167	6	10	theme	O	953:953	arg1	elements					962:969	C, N, O and Se elements	947:969	C, N, O and Se elements	947:969	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	5	11	theme	orange-red	848:857	arg1	particles					888:896	zero-valent and orange-red relatively uniform spherical particles	832:896	zero-valent and orange-red relatively uniform spherical particles	832:896	The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared.
36555167	8	12	theme	inflammatory	1194:1205	arg1	cytokines					1207:1215	colonic inflammatory cytokines	1186:1215	colonic inflammatory cytokines	1186:1215	LCS-SeNPs significantly reduced serum and colonic inflammatory cytokines TNF-α and IL-6 levels.
36555167	6	13	theme	N	950:950	arg1	elements					962:969	C, N, O and Se elements	947:969	C, N, O and Se elements	947:969	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	14	theme	C	947:947	arg1	elements					962:969	C, N, O and Se elements	947:969	C, N, O and Se elements	947:969	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	4	15	theme	process	734:740	arg1	protection					598:607	The protection	594:607	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	594:696	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	4	15	theme	process	734:740	arg1	mechanisms					715:724	the inherent mechanisms	702:724	the inherent mechanisms of this process	702:740	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	0	16	from	Effect	92:97	arg1	Colitis					125:131	DSS-Induced Ulcerative Colitis	102:131	DSS-Induced Ulcerative Colitis in Mice	102:139	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	0	17	theme	Selenium	61:68	arg1	Nanoparticles					70:82	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	5	18	theme	nm	828:829	arg1	diameter					812:819	an average diameter	801:819	an average diameter of 198 nm	801:829	The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared.
36555167	0	19	from	Characterization	11:26	arg1	Colitis					125:131	DSS-Induced Ulcerative Colitis	102:131	DSS-Induced Ulcerative Colitis in Mice	102:139	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	10	20	theme	signaling	1455:1463	arg1	pathway					1465:1471	the NF-κB signaling pathway	1445:1471	the NF-κB signaling pathway	1445:1471	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	4	21	theme	inherent	706:713	arg1	mechanisms					715:724	the inherent mechanisms	702:724	the inherent mechanisms of this process	702:740	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	0	22	from	Synthesis	0:8	arg1	Colitis					125:131	DSS-Induced Ulcerative Colitis	102:131	DSS-Induced Ulcerative Colitis in Mice	102:139	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	9	23	theme	enzyme	1293:1298	arg1	levels					1307:1312	antioxidant enzyme GSH-Px levels	1281:1312	antioxidant enzyme GSH-Px levels	1281:1312	Moreover, LCS-SeNPs remarkably increased antioxidant enzyme GSH-Px levels in serum and colonic tissue.
36555167	3	24	theme	element	448:454	arg1	state					456:460	element state	448:460	element state	448:460	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	6	25	dep	elements	1017:1024	arg1	Se					1038:1039	Se	1038:1039	Se	1038:1039	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	25	dep	elements	1017:1024	arg1	O					1032:1032	O	1032:1032	O	1032:1032	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	25	dep	elements	1017:1024	arg1	elements					1017:1024	the four elements C, N, O and Se	1008:1039	the four elements C, N, O and Se	1008:1039	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	25	dep	elements	1017:1024	arg1	N					1029:1029	N	1029:1029	N	1029:1029	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	25	dep	elements	1017:1024	arg1	C					1026:1026	C	1026:1026	C	1026:1026	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	0	26	theme	DSS-Induced	102:112	arg1	Colitis					125:131	DSS-Induced Ulcerative Colitis	102:131	DSS-Induced Ulcerative Colitis in Mice	102:139	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	2	27	theme	acetic	425:430	arg1	acid					432:435	acetic acid	425:435	acetic acid	425:435	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	2	28	theme	selenite	412:419	arg1	system					395:400	a system	393:400	a system of sodium selenite and acetic acid	393:435	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	7	29	theme	intestinal	1112:1121	arg1	dysfunction					1131:1141	intestinal barrier dysfunction	1112:1141	intestinal barrier dysfunction	1112:1141	LCS-SeNPs reduced colon injury and inflammation symptoms and improved intestinal barrier dysfunction.
36555167	9	30	theme	colonic	1327:1333	arg1	tissue					1335:1340	colonic tissue	1327:1340	colonic tissue	1327:1340	Moreover, LCS-SeNPs remarkably increased antioxidant enzyme GSH-Px levels in serum and colonic tissue.
36555167	10	31	theme	associated	1500:1509	arg1	stress					1521:1526	inflammatory associated oxidative stress	1487:1526	inflammatory associated oxidative stress	1487:1526	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	4	32	theme	-induced	658:665	arg1	dysfunction					686:696	dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	630:696	dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	630:696	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	0	33	theme	Low	31:33	arg1	Weight					45:50	Low Molecular Weight	31:50	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	10	34	from	studies	1351:1357	arg1	pathways					1375:1382	inflammatory pathways	1362:1382	inflammatory pathways	1362:1382	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	10	35	theme	signaling	1545:1553	arg1	pathway					1555:1561	the Nrf2 signaling pathway	1536:1561	the Nrf2 signaling pathway	1536:1561	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	8	36	dep	serum	1176:1180	arg1	levels					1232:1237	TNF-α and IL-6 levels	1217:1237	levels	1232:1237	LCS-SeNPs significantly reduced serum and colonic inflammatory cytokines TNF-α and IL-6 levels.
36555167	2	37	theme	present	255:261	arg1	study					263:267	the present study	251:267	the present study	251:267	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	11	38	theme	promising	1608:1616	arg1	species					1627:1633	a promising selenium species	1606:1633	a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases	1606:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	11	38	theme	promising	1608:1616	arg1	LCS-SeNPs					1592:1600	LCS-SeNPs	1592:1600	LCS-SeNPs	1592:1600	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	2	39	theme	chitosan	328:335	arg1	LCS-SeNPs					361:369	LCS-SeNPs	361:369	LCS-SeNPs	361:369	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	2	39	theme	chitosan	328:335	arg1	nanoparticles					346:358	chitosan selenium nanoparticles	328:358	Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)	307:370	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	0	40	from	Colitis	125:131	arg1	Mice					136:139	Mice	136:139	Mice	136:139	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	11	41	theme	oxidative	1683:1691	arg1	diseases					1732:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	7	42	theme	colon	1060:1064	arg1	injury					1066:1071	colon injury	1060:1071	colon injury	1060:1071	LCS-SeNPs reduced colon injury and inflammation symptoms and improved intestinal barrier dysfunction.
36555167	11	43	theme	potential	1640:1648	arg1	applications					1650:1661	potential applications	1640:1661	potential applications in the treatment of oxidative stress related inflammatory intestinal diseases	1640:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	3	44	theme	elementary	478:487	arg1	composition					489:499	elementary composition	478:499	elementary composition	478:499	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	2	45	theme	selenium	284:291	arg1	nanoparticle					293:304	selenium nanoparticle	284:304	selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs))	284:371	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	11	46	with	species	1627:1633	arg1	applications					1650:1661	potential applications	1640:1661	potential applications in the treatment of oxidative stress related inflammatory intestinal diseases	1640:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	11	47	theme	intestinal	1721:1730	arg1	diseases					1732:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	10	48	theme	inflammatory	1362:1373	arg1	pathways					1375:1382	inflammatory pathways	1362:1382	inflammatory pathways	1362:1382	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	4	49	theme	barrier	678:684	arg1	dysfunction					686:696	dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	630:696	dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	630:696	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	1	50	theme	extensive	180:188	arg1	attention					190:198	extensive attention	180:198	extensive attention	180:198	Selenium nanoparticles have attracted extensive attention due to their good bioavailability and activity.
36555167	5	51	theme	average	804:810	arg1	diameter					812:819	an average diameter	801:819	an average diameter of 198 nm	801:829	The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared.
36555167	11	52	theme	related	1700:1706	arg1	diseases					1732:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	oxidative stress related inflammatory intestinal diseases	1683:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	10	53	theme	DSS-induced	1417:1427	arg1	colitis					1429:1435	DSS-induced colitis	1417:1435	DSS-induced colitis	1417:1435	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	2	54	theme	molecular	311:319	arg1	weight					321:326	Low molecular weight	307:326	Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)	307:370	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	11	55	from	applications	1650:1661	arg1	treatment					1670:1678	the treatment	1666:1678	the treatment of oxidative stress related inflammatory intestinal diseases	1666:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	0	56	theme	Weight	45:50	arg1	Nanoparticles					70:82	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	4	57	theme	intestinal	667:676	arg1	dysfunction					686:696	dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	630:696	dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	630:696	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	3	58	theme	various	542:548	arg1	measurements					580:591	various spectroscopic and microscopic measurements	542:591	various spectroscopic and microscopic measurements	542:591	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	3	59	theme	microscopic	568:578	arg1	measurements					580:591	various spectroscopic and microscopic measurements	542:591	various spectroscopic and microscopic measurements	542:591	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	8	60	theme	colonic	1186:1192	arg1	cytokines					1207:1215	colonic inflammatory cytokines	1186:1215	colonic inflammatory cytokines	1186:1215	LCS-SeNPs significantly reduced serum and colonic inflammatory cytokines TNF-α and IL-6 levels.
36555167	2	61	dep	nanoparticle	293:304	arg1	weight					321:326	Low molecular weight	307:326	Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)	307:370	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	0	62	theme	Nanoparticles	70:82	arg1	Characterization					11:26	Characterization	11:26	Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles	11:82	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	0	62	theme	Nanoparticles	70:82	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	0	62	theme	Nanoparticles	70:82	arg1	Effect					92:97	Its Effect	88:97	Its Effect on DSS-Induced Ulcerative Colitis in Mice	88:139	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	1	63	theme	Selenium	142:149	arg1	nanoparticles					151:163	Selenium nanoparticles	142:163	Selenium nanoparticles	142:163	Selenium nanoparticles have attracted extensive attention due to their good bioavailability and activity.
36555167	10	64	theme	NF-κB	1449:1453	arg1	pathway					1465:1471	the NF-κB signaling pathway	1445:1471	the NF-κB signaling pathway	1445:1471	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	9	65	theme	antioxidant	1281:1291	arg1	levels					1307:1312	antioxidant enzyme GSH-Px levels	1281:1312	antioxidant enzyme GSH-Px levels	1281:1312	Moreover, LCS-SeNPs remarkably increased antioxidant enzyme GSH-Px levels in serum and colonic tissue.
36555167	2	66	theme	nanoparticle	293:304	arg1	form					276:279	a new form	270:279	a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs))	270:371	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	0	67	theme	Ulcerative	114:123	arg1	Colitis					125:131	DSS-Induced Ulcerative Colitis	102:131	DSS-Induced Ulcerative Colitis in Mice	102:139	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	9	68	theme	GSH-Px	1300:1305	arg1	levels					1307:1312	antioxidant enzyme GSH-Px levels	1281:1312	antioxidant enzyme GSH-Px levels	1281:1312	Moreover, LCS-SeNPs remarkably increased antioxidant enzyme GSH-Px levels in serum and colonic tissue.
36555167	1	69	theme	good	213:216	arg1	bioavailability					218:232	their good bioavailability	207:232	their good bioavailability	207:232	Selenium nanoparticles have attracted extensive attention due to their good bioavailability and activity.
36555167	7	70	theme	barrier	1123:1129	arg1	dysfunction					1131:1141	intestinal barrier dysfunction	1112:1141	intestinal barrier dysfunction	1112:1141	LCS-SeNPs reduced colon injury and inflammation symptoms and improved intestinal barrier dysfunction.
36555167	2	71	theme	acid	432:435	arg1	system					395:400	a system	393:400	a system of sodium selenite and acetic acid	393:435	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	2	72	dep	weight	321:326	arg1	LCS-SeNPs					361:369	LCS-SeNPs	361:369	LCS-SeNPs	361:369	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	2	72	dep	weight	321:326	arg1	nanoparticles					346:358	chitosan selenium nanoparticles	328:358	Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)	307:370	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	6	73	theme	elements	1017:1024	arg1	O					1032:1032	O	1032:1032	O	1032:1032	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	73	theme	elements	1017:1024	arg1	elements					1017:1024	the four elements C, N, O and Se	1008:1039	the four elements C, N, O and Se	1008:1039	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	73	theme	elements	1017:1024	arg1	Se					1038:1039	Se	1038:1039	Se	1038:1039	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	73	theme	elements	1017:1024	arg1	C					1026:1026	C	1026:1026	C	1026:1026	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	73	theme	elements	1017:1024	arg1	%					1003:1003	39.03%	998:1003	39.03% of the four elements C, N, O and Se	998:1039	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	6	73	theme	elements	1017:1024	arg1	N					1029:1029	N	1029:1029	N	1029:1029	LCS-SeNPs were mainly composed of C, N, O and Se elements, of which Se accounted for 39.03% of the four elements C, N, O and Se.
36555167	10	74	theme	oxidative	1511:1519	arg1	stress					1521:1526	inflammatory associated oxidative stress	1487:1526	inflammatory associated oxidative stress	1487:1526	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	2	75	theme	sodium	405:410	arg1	selenite					412:419	sodium selenite	405:419	sodium selenite	405:419	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	0	76	theme	Molecular	35:43	arg1	Weight					45:50	Low Molecular Weight	31:50	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	10	77	theme	inflammatory	1487:1498	arg1	stress					1521:1526	inflammatory associated oxidative stress	1487:1526	inflammatory associated oxidative stress	1487:1526	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	11	78	theme	diseases	1732:1739	arg1	treatment					1670:1678	the treatment	1666:1678	the treatment of oxidative stress related inflammatory intestinal diseases	1666:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	11	79	theme	selenium	1618:1625	arg1	species					1627:1633	a promising selenium species	1606:1633	a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases	1606:1739	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	11	79	theme	selenium	1618:1625	arg1	LCS-SeNPs					1592:1600	LCS-SeNPs	1592:1600	LCS-SeNPs	1592:1600	Our findings suggested that LCS-SeNPs are a promising selenium species with potential applications in the treatment of oxidative stress related inflammatory intestinal diseases.
36555167	0	80	theme	Chitosan	52:59	arg1	Nanoparticles					70:82	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Low Molecular Weight Chitosan Selenium Nanoparticles	31:82	Synthesis, Characterization of Low Molecular Weight Chitosan Selenium Nanoparticles and Its Effect on DSS-Induced Ulcerative Colitis in Mice.
36555167	10	81	theme	Nrf2	1540:1543	arg1	pathway					1555:1561	the Nrf2 signaling pathway	1536:1561	the Nrf2 signaling pathway	1536:1561	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	5	82	dep	uniform	870:876	arg1	spherical					878:886	spherical	878:886	spherical	878:886	The results showed that LCS-SeNPs, with an average diameter of 198 nm, zero-valent and orange-red relatively uniform spherical particles were prepared.
36555167	2	83	theme	selenium	337:344	arg1	LCS-SeNPs					361:369	LCS-SeNPs	361:369	LCS-SeNPs	361:369	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	2	83	theme	selenium	337:344	arg1	nanoparticles					346:358	chitosan selenium nanoparticles	328:358	Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)	307:370	In the present study, a new form of selenium nanoparticle (Low molecular weight chitosan selenium nanoparticles (LCS-SeNPs)) were synthesized in a system of sodium selenite and acetic acid.
36555167	4	84	theme	LCS-SeNPs	612:620	arg1	protection					598:607	The protection	594:607	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction	594:696	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	4	84	theme	LCS-SeNPs	612:620	arg1	mechanisms					715:724	the inherent mechanisms	702:724	the inherent mechanisms of this process	702:740	The protection of LCS-SeNPs against dextran sulfate sodium (DSS)-induced intestinal barrier dysfunction and the inherent mechanisms of this process were investigated.
36555167	10	85	theme	Further	1343:1349	arg1	studies					1351:1357	Further studies	1343:1357	Further studies on inflammatory pathways	1343:1382	Further studies on inflammatory pathways showed that LCS-SeNPs alleviated DSS-induced colitis through the NF-κB signaling pathway, and relieved inflammatory associated oxidative stress through the Nrf2 signaling pathway.
36555167	3	86	theme	LCS-SeNPs	504:512	arg1	morphology					463:472	morphology	463:472	morphology	463:472	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	3	86	theme	LCS-SeNPs	504:512	arg1	size					442:445	The size	438:445	The size	438:445	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	3	86	theme	LCS-SeNPs	504:512	arg1	state					456:460	element state	448:460	element state	448:460	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36555167	3	86	theme	LCS-SeNPs	504:512	arg1	composition					489:499	elementary composition	478:499	elementary composition	478:499	The size, element state, morphology and elementary composition of LCS-SeNPs were characterized by using various spectroscopic and microscopic measurements.
36710178	11	0	theme	Further	1985:1991	arg1	studies					1993:1999	Further studies	1985:1999	Further studies	1985:1999	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	2	1	from	seasons	540:546	arg1	cows					518:521	69,252 first parity cows	498:521	69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds	498:606	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	14	2	from	terms	2490:2494	arg1	population					2537:2546	the OAD milking cow population	2517:2546	the OAD milking cow population	2517:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	3	3	dep	production	636:645	arg1	traits					661:666	traits	661:666	traits	661:666	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	10	4	from	in-calf	1809:1815	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	11	5	from	percentage	2054:2063	arg1	system					2105:2110	the OAD milking system	2089:2110	the OAD milking system	2089:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	1	6	theme	milk	277:280	arg1	production					282:291	milk production	277:291	milk production	277:291	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	2	7	theme	milking	594:600	arg1	herds					602:606	113 OAD and 531 TAD milking herds	574:606	herds	602:606	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	3	8	theme	single-trait	690:701	arg1	models					710:715	single-trait animal models	690:715	single-trait animal models	690:715	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	5	9	from	low	973:975	arg1	populations					1009:1019	both OAD and TAD milking cow populations	980:1019	populations	1009:1019	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	5	9	from	low	973:975	arg1	OAD					985:987	both OAD and TAD milking cow populations	980:1019	OAD	985:987	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	12	10	dep	OAD	2182:2184	arg1	populations					2202:2212	milking populations	2194:2212	milking populations	2194:2212	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	7	11	theme	genetic	1354:1360	arg1	correlations					1362:1373	the genetic correlations	1350:1373	the genetic correlations	1350:1373	In both populations, phenotypic correlations between milk production and fertility traits were close to zero, and most of the genetic correlations were antagonistic.
36710178	0	12	theme	New	155:157	arg1	Zealand					159:165	New Zealand	155:165	New Zealand	155:165	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	1	13	theme	study	191:195	arg1	objectives					172:181	The objectives	168:181	The objectives of this study	168:195	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	14	14	theme	cow	2533:2535	arg1	population					2537:2546	the OAD milking cow population	2517:2546	the OAD milking cow population	2517:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	13	15	theme	genetic	2305:2311	arg1	correlations					2313:2324	genetic correlations	2305:2324	genetic correlations	2305:2324	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	10	16	theme	calving	1870:1876	arg1	rate					1878:1881	6-wk calving rate	1865:1881	6-wk calving rate	1865:1881	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	0	17	theme	twice-daily	127:137	arg1	production					64:73	milk production	59:73	milk production	59:73	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	0	17	theme	twice-daily	127:137	arg1	traits					89:94	fertility traits	79:94	fertility traits	79:94	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	13	18	theme	Genetic	2215:2221	arg1	correlations					2223:2234	Genetic correlations	2215:2234	Genetic correlations between fertility traits	2215:2259	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	10	19	from	calving	1852:1858	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	14	20	theme	OAD	2521:2523	arg1	population					2537:2546	the OAD milking cow population	2517:2546	the OAD milking cow population	2517:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	8	21	theme	OAD	1397:1399	arg1	cows					1409:1412	OAD milking cows	1397:1412	OAD milking cows	1397:1412	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	10	22	theme	cow	1902:1904	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	3	23	theme	correlations	758:769	arg1	estimates					722:730	estimates	722:730	estimates of genetic and phenotypic correlations	722:769	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	1	24	from	heritabilities	258:271	arg1	cows					356:359	spring-calved once-daily (OAD) milking cows	317:359	spring-calved once-daily (OAD) milking cows	317:359	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	9	25	theme	milk	1657:1660	arg1	yields					1674:1679	milk and lactose yields	1657:1679	yields	1674:1679	Interval from first service to conception was negatively genetically correlated with milk and lactose yields in OAD milking cows.
36710178	0	26	from	production	64:73	arg1	Zealand					159:165	New Zealand	155:165	New Zealand	155:165	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	10	27	theme	TAD	1890:1892	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	1	28	from	correlations	241:252	arg1	cows					356:359	spring-calved once-daily (OAD) milking cows	317:359	spring-calved once-daily (OAD) milking cows	317:359	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	9	29	theme	lactose	1666:1672	arg1	yields					1674:1679	milk and lactose yields	1657:1679	yields	1674:1679	Interval from first service to conception was negatively genetically correlated with milk and lactose yields in OAD milking cows.
36710178	8	30	theme	genetic	1415:1421	arg1	correlations					1423:1434	genetic correlations	1415:1434	genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate	1415:1550	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	6	31	theme	genetic	1147:1153	arg1	correlations					1155:1166	phenotypic and genetic correlations	1132:1166	phenotypic and genetic correlations among production traits	1132:1190	Estimates of phenotypic and genetic correlations among production traits were consistent across populations.
36710178	11	32	from	traits	2079:2084	arg1	system					2105:2110	the OAD milking system	2089:2110	the OAD milking system	2089:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	3	33	theme	bivariate	793:801	arg1	models					810:815	bivariate animal models	793:815	bivariate animal models	793:815	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	10	34	theme	6-wk	1773:1776	arg1	submission					1778:1787	3-wk and 6-wk submission	1764:1787	3-wk and 6-wk submission	1764:1787	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	9	35	from	yields	1674:1679	arg1	cows					1696:1699	OAD milking cows	1684:1699	OAD milking cows	1684:1699	Interval from first service to conception was negatively genetically correlated with milk and lactose yields in OAD milking cows.
36710178	6	36	theme	phenotypic	1132:1141	arg1	correlations					1155:1166	phenotypic and genetic correlations	1132:1166	phenotypic and genetic correlations among production traits	1132:1190	Estimates of phenotypic and genetic correlations among production traits were consistent across populations.
36710178	4	37	from	0.61	882:885	arg1	OAD					890:892	OAD	890:892	OAD	890:892	Heritability estimates of production traits varied from 0.26 to 0.61 in OAD and from 0.13 to 0.63 in TAD.
36710178	10	38	theme	3-wk	1764:1767	arg1	submission					1778:1787	3-wk and 6-wk submission	1764:1787	3-wk and 6-wk submission	1764:1787	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	13	39	from	cows	2356:2359	arg1	strong					2346:2351	strong	2346:2351	strong	2346:2351	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	9	40	theme	milking	1688:1694	arg1	cows					1696:1699	OAD milking cows	1684:1699	OAD milking cows	1684:1699	Interval from first service to conception was negatively genetically correlated with milk and lactose yields in OAD milking cows.
36710178	13	41	from	strong	2346:2351	arg1	cows					2356:2359	cows	2356:2359	cows	2356:2359	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	12	42	from	OAD	2182:2184	arg1	similar					2171:2177	similar	2171:2177	similar	2171:2177	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	12	42	from	OAD	2182:2184	arg1	correlations					2128:2139	The phenotypic correlations	2113:2139	The phenotypic correlations between fertility traits	2113:2164	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	0	43	theme	fertility	79:87	arg1	traits					89:94	fertility traits	79:94	fertility traits	79:94	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	1	44	theme	milking	348:354	arg1	cows					356:359	spring-calved once-daily (OAD) milking cows	317:359	spring-calved once-daily (OAD) milking cows	317:359	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	2	45	theme	first	505:509	arg1	cows					518:521	69,252 first parity cows	498:521	69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds	498:606	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	8	46	theme	yields	1456:1461	arg1	correlations					1423:1434	genetic correlations	1415:1434	genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate	1415:1550	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	4	47	dep	0.63	911:914	arg1	to					908:909	to	908:909	to	908:909	Heritability estimates of production traits varied from 0.26 to 0.61 in OAD and from 0.13 to 0.63 in TAD.
36710178	10	48	from	rate	1878:1881	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	5	49	theme	fertility	951:959	arg1	traits					961:966	fertility traits	951:966	fertility traits	951:966	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	0	50	theme	once-daily	113:122	arg1	production					64:73	milk production	59:73	milk production	59:73	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	0	50	theme	once-daily	113:122	arg1	traits					89:94	fertility traits	79:94	fertility traits	79:94	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	1	51	theme	whole	369:373	arg1	season					375:380	the whole season	365:380	the whole season	365:380	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	13	52	theme	strong	2346:2351	arg1	OAD					2368:2370	weak to strong in cows milked OAD	2338:2370	weak to strong in cows milked OAD	2338:2370	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	7	53	theme	fertility	1301:1309	arg1	traits					1311:1316	fertility traits	1301:1316	fertility traits	1301:1316	In both populations, phenotypic correlations between milk production and fertility traits were close to zero, and most of the genetic correlations were antagonistic.
36710178	11	54	theme	fertility	2069:2077	arg1	traits					2079:2084	fertility traits	2069:2084	fertility traits in the OAD milking system	2069:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	5	55	dep	0.08	1088:1091	arg1	to					1085:1086	to	1085:1086	to	1085:1086	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	5	56	theme	milking	1101:1107	arg1	regimens					1109:1116	milking regimens	1101:1116	milking regimens	1101:1116	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	4	57	theme	production	844:853	arg1	traits					855:860	production traits	844:860	production traits	844:860	Heritability estimates of production traits varied from 0.26 to 0.61 in OAD and from 0.13 to 0.63 in TAD.
36710178	7	58	from	zero	1332:1335	arg1	populations					1236:1246	both populations	1231:1246	both populations	1231:1246	In both populations, phenotypic correlations between milk production and fertility traits were close to zero, and most of the genetic correlations were antagonistic.
36710178	11	59	theme	percentage	2054:2063	arg1	relationship					2030:2041	the relationship	2026:2041	the relationship of protein percentage and fertility traits in the OAD milking system	2026:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	2	60	from	2017-2018	561:569	arg1	herds					602:606	113 OAD and 531 TAD milking herds	574:606	herds	602:606	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	2	60	from	2017-2018	561:569	arg1	OAD					578:580	113 OAD and 531 TAD milking herds	574:606	OAD	578:580	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	12	61	theme	phenotypic	2117:2126	arg1	similar					2171:2177	similar	2171:2177	similar	2171:2177	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	12	61	theme	phenotypic	2117:2126	arg1	correlations					2128:2139	The phenotypic correlations	2113:2139	The phenotypic correlations between fertility traits	2113:2164	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	0	62	theme	genetic	19:25	arg1	correlations					42:53	genetic and phenotypic correlations	19:53	genetic and phenotypic correlations	19:53	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	1	63	theme	fertility	297:305	arg1	traits					307:312	fertility traits	297:312	fertility traits	297:312	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	10	64	from	submission	1778:1787	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	11	65	theme	OAD	2093:2095	arg1	system					2105:2110	the OAD milking system	2089:2110	the OAD milking system	2089:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	0	66	theme	phenotypic	31:40	arg1	correlations					42:53	genetic and phenotypic correlations	19:53	genetic and phenotypic correlations	19:53	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	5	67	theme	TAD	993:995	arg1	populations					1009:1019	both OAD and TAD milking cow populations	980:1019	populations	1009:1019	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	7	68	theme	phenotypic	1249:1258	arg1	correlations					1260:1271	phenotypic correlations	1249:1271	phenotypic correlations between milk production and fertility traits	1249:1316	In both populations, phenotypic correlations between milk production and fertility traits were close to zero, and most of the genetic correlations were antagonistic.
36710178	5	69	dep	0.10	1066:1069	arg1	to					1063:1064	to	1063:1064	to	1063:1064	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	1	70	theme	once-daily	331:340	arg1	cows					356:359	spring-calved once-daily (OAD) milking cows	317:359	spring-calved once-daily (OAD) milking cows	317:359	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	1	71	theme	OAD	343:345	arg1	cows					356:359	spring-calved once-daily (OAD) milking cows	317:359	spring-calved once-daily (OAD) milking cows	317:359	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	5	72	theme	cow	1005:1007	arg1	populations					1009:1019	both OAD and TAD milking cow populations	980:1019	populations	1009:1019	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	10	73	theme	cow	1969:1971	arg1	population					1973:1982	the OAD milking cow population	1953:1982	the OAD milking cow population	1953:1982	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	1	74	theme	genetic	218:224	arg1	correlations					241:252	the genetic and phenotypic correlations	214:252	the genetic and phenotypic correlations	214:252	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	10	75	from	low	1946:1948	arg1	population					1973:1982	the OAD milking cow population	1953:1982	the OAD milking cow population	1953:1982	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	10	76	theme	OAD	1957:1959	arg1	population					1973:1982	the OAD milking cow population	1953:1982	the OAD milking cow population	1953:1982	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	5	77	dep	OAD	1053:1055	arg1	TAD					1075:1077	TAD	1075:1077	TAD	1075:1077	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	5	77	dep	OAD	1053:1055	arg1	0.10					1066:1069	0.10	1066:1069	0.10	1066:1069	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	5	77	dep	OAD	1053:1055	arg1	0.08					1088:1091	0.08	1088:1091	0.08	1088:1091	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	10	78	theme	6-wk	1804:1807	arg1	in-calf					1809:1815	6-wk in-calf	1804:1815	6-wk in-calf	1804:1815	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	13	79	from	strong	2266:2271	arg1	cows					2284:2287	cows	2284:2287	cows milked TAD	2284:2298	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	1	80	theme	phenotypic	230:239	arg1	correlations					241:252	the genetic and phenotypic correlations	214:252	the genetic and phenotypic correlations	214:252	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	8	81	theme	calving	1539:1545	arg1	rate					1547:1550	6-wk calving rate	1534:1550	6-wk calving rate	1534:1550	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	12	82	from	TAD	2190:2192	arg1	similar					2171:2177	similar	2171:2177	similar	2171:2177	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	12	82	from	TAD	2190:2192	arg1	correlations					2128:2139	The phenotypic correlations	2113:2139	The phenotypic correlations between fertility traits	2113:2164	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	10	83	theme	3-wk	1847:1850	arg1	calving					1852:1858	3-wk calving	1847:1858	3-wk calving	1847:1858	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	8	84	with	correlations	1423:1434	arg1	start					1472:1476	the start	1468:1476	the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate	1468:1550	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	10	85	from	in-calf	1795:1801	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	14	86	theme	Further	2373:2379	arg1	research					2381:2388	Further research	2373:2388	Further research	2373:2388	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	14	87	from	regimen	2458:2464	arg1	terms					2490:2494	terms	2490:2494	terms of sire selection in the OAD milking cow population	2490:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	9	88	theme	first	1586:1590	arg1	service					1592:1598	first service	1586:1598	first service	1586:1598	Interval from first service to conception was negatively genetically correlated with milk and lactose yields in OAD milking cows.
36710178	5	89	from	OAD	985:987	arg1	low					973:975	low	973:975	low	973:975	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	13	90	theme	fertility	2244:2252	arg1	traits					2254:2259	fertility traits	2244:2259	fertility traits	2244:2259	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	2	91	theme	TAD	590:592	arg1	herds					602:606	113 OAD and 531 TAD milking herds	574:606	herds	602:606	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	3	92	theme	animal	703:708	arg1	models					710:715	single-trait animal models	690:715	single-trait animal models	690:715	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	10	93	from	service	1824:1830	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	4	94	theme	traits	855:860	arg1	estimates					831:839	Heritability estimates	818:839	Heritability estimates of production traits	818:860	Heritability estimates of production traits varied from 0.26 to 0.61 in OAD and from 0.13 to 0.63 in TAD.
36710178	11	95	from	system	2105:2110	arg1	relationship					2030:2041	the relationship	2026:2041	the relationship of protein percentage and fertility traits in the OAD milking system	2026:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	14	96	from	population	2537:2546	arg1	terms					2490:2494	terms	2490:2494	terms of sire selection in the OAD milking cow population	2490:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	3	97	theme	genetic	735:741	arg1	correlations					758:769	genetic and phenotypic correlations	735:769	genetic and phenotypic correlations	735:769	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	0	98	theme	milking	139:145	arg1	cows					147:150	milking cows	139:150	milking cows	139:150	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	10	99	theme	Protein	1702:1708	arg1	percentage					1710:1719	Protein percentage	1702:1719	Protein percentage	1702:1719	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	9	100	from	service	1592:1598	arg1	Interval					1572:1579	Interval	1572:1579	Interval from first service to conception	1572:1612	Interval from first service to conception was negatively genetically correlated with milk and lactose yields in OAD milking cows.
36710178	0	101	from	traits	89:94	arg1	Zealand					159:165	New Zealand	155:165	New Zealand	155:165	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	14	102	from	selection	2504:2512	arg1	population					2537:2546	the OAD milking cow population	2517:2546	the OAD milking cow population	2517:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	10	103	theme	6-wk	1865:1868	arg1	rate					1878:1881	6-wk calving rate	1865:1881	6-wk calving rate	1865:1881	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	1	104	theme	New	385:387	arg1	Zealand					389:395	New Zealand	385:395	New Zealand	385:395	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	2	105	dep	2017-2018	561:569	arg1	to					558:559	to	558:559	to	558:559	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	2	106	theme	parity	511:516	arg1	cows					518:521	69,252 first parity cows	498:521	69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds	498:606	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	6	107	theme	production	1174:1183	arg1	traits					1185:1190	production traits	1174:1190	production traits	1174:1190	Estimates of phenotypic and genetic correlations among production traits were consistent across populations.
36710178	10	108	theme	milking	1894:1900	arg1	population					1906:1915	the TAD milking cow population	1886:1915	the TAD milking cow population	1886:1915	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	8	109	from	zero	1566:1569	arg1	cows					1409:1412	OAD milking cows	1397:1412	OAD milking cows	1397:1412	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	11	110	from	relationship	2030:2041	arg1	system					2105:2110	the OAD milking system	2089:2110	the OAD milking system	2089:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	12	111	theme	milking	2194:2200	arg1	populations					2202:2212	milking populations	2194:2212	milking populations	2194:2212	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	14	112	theme	selection	2504:2512	arg1	terms					2490:2494	terms	2490:2494	terms of sire selection in the OAD milking cow population	2490:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	6	113	theme	correlations	1155:1166	arg1	Estimates					1119:1127	Estimates	1119:1127	Estimates of phenotypic and genetic correlations among production traits	1119:1190	Estimates of phenotypic and genetic correlations among production traits were consistent across populations.
36710178	13	114	dep	strong	2346:2351	arg1	to					2343:2344	to	2343:2344	to	2343:2344	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	3	115	theme	phenotypic	747:756	arg1	correlations					758:769	genetic and phenotypic correlations	735:769	genetic and phenotypic correlations	735:769	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	13	116	theme	milked	2361:2366	arg1	OAD					2368:2370	weak to strong in cows milked OAD	2338:2370	weak to strong in cows milked OAD	2338:2370	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	2	117	theme	calving	532:538	arg1	seasons					540:546	the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds	528:606	the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds	528:606	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	8	118	theme	milk	1439:1442	arg1	correlations					1423:1434	genetic correlations	1415:1434	genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate	1415:1550	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	3	119	theme	animal	803:808	arg1	models					810:815	bivariate animal models	793:815	bivariate animal models	793:815	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	9	120	theme	OAD	1684:1686	arg1	cows					1696:1699	OAD milking cows	1684:1699	OAD milking cows	1684:1699	Interval from first service to conception was negatively genetically correlated with milk and lactose yields in OAD milking cows.
36710178	8	121	theme	lactose	1448:1454	arg1	yields					1456:1461	lactose yields	1448:1461	lactose yields	1448:1461	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	5	122	from	populations	1009:1019	arg1	low					973:975	low	973:975	low	973:975	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	14	123	theme	milking	2525:2531	arg1	population					2537:2546	the OAD milking cow population	2517:2546	the OAD milking cow population	2517:2546	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	14	124	theme	fertility	2470:2478	arg1	traits					2480:2485	fertility traits	2470:2485	fertility traits	2470:2485	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	8	125	theme	milking	1401:1407	arg1	cows					1409:1412	OAD milking cows	1397:1412	OAD milking cows	1397:1412	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	0	126	theme	spring-calved	99:111	arg1	once-daily					113:122	spring-calved once-daily	99:122	spring-calved once-daily	99:122	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	0	127	dep	once-daily	113:122	arg1	cows					147:150	milking cows	139:150	milking cows	139:150	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	12	128	from	similar	2171:2177	arg1	TAD					2190:2192	TAD	2190:2192	TAD	2190:2192	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	12	128	from	similar	2171:2177	arg1	OAD					2182:2184	OAD	2182:2184	OAD	2182:2184	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	8	129	theme	mating	1481:1486	arg1	start					1472:1476	the start	1468:1476	the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate	1468:1550	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	11	130	theme	protein	2046:2052	arg1	percentage					2054:2063	protein percentage	2046:2063	protein percentage	2046:2063	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	12	131	theme	fertility	2149:2157	arg1	traits					2159:2164	fertility traits	2149:2164	fertility traits	2149:2164	The phenotypic correlations between fertility traits were similar in OAD and TAD milking populations.
36710178	7	132	theme	milk	1281:1284	arg1	production					1286:1295	milk production	1281:1295	milk production	1281:1295	In both populations, phenotypic correlations between milk production and fertility traits were close to zero, and most of the genetic correlations were antagonistic.
36710178	11	133	theme	traits	2079:2084	arg1	relationship					2030:2041	the relationship	2026:2041	the relationship of protein percentage and fertility traits in the OAD milking system	2026:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	14	134	dep	selection	2504:2512	arg1	sire					2499:2502	sire	2499:2502	sire	2499:2502	Further research is required to evaluate the interaction between genotype by milking regimen for fertility traits in terms of sire selection in the OAD milking cow population.
36710178	10	135	from	population	1973:1982	arg1	low					1946:1948	low	1946:1948	low	1946:1948	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	5	136	theme	Heritability	924:935	arg1	estimates					937:945	Heritability estimates	924:945	Heritability estimates for fertility traits	924:966	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	8	137	theme	6-wk	1503:1506	arg1	in-calf					1508:1514	6-wk in-calf	1503:1514	6-wk in-calf	1503:1514	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	3	138	theme	Heritability	609:620	arg1	estimates					622:630	Heritability estimates	609:630	Heritability estimates for production and fertility traits	609:666	Heritability estimates for production and fertility traits were obtained through single-trait animal models, and estimates of genetic and phenotypic correlations were obtained through bivariate animal models.
36710178	13	139	from	cows	2284:2287	arg1	strong					2266:2271	strong	2266:2271	strong	2266:2271	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	4	140	dep	0.61	882:885	arg1	to					879:880	to	879:880	to	879:880	Heritability estimates of production traits varied from 0.26 to 0.61 in OAD and from 0.13 to 0.63 in TAD.
36710178	11	141	theme	milking	2097:2103	arg1	system					2105:2110	the OAD milking system	2089:2110	the OAD milking system	2089:2110	Further studies are needed to understand the relationship of protein percentage and fertility traits in the OAD milking system.
36710178	10	142	theme	3-wk	1790:1793	arg1	in-calf					1795:1801	3-wk in-calf	1790:1801	3-wk in-calf	1790:1801	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	13	143	dep	strong	2266:2271	arg1	≥0.70					2274:2278	≥0.70	2274:2278	≥0.70	2274:2278	Genetic correlations between fertility traits were strong (≥0.70) in cows milked TAD, but genetic correlations varied from weak to strong in cows milked OAD.
36710178	0	144	theme	milk	59:62	arg1	production					64:73	milk production	59:73	milk production	59:73	Heritabilities and genetic and phenotypic correlations for milk production and fertility traits of spring-calved once-daily or twice-daily milking cows in New Zealand.
36710178	1	145	theme	spring-calved	317:329	arg1	cows					356:359	spring-calved once-daily (OAD) milking cows	317:359	spring-calved once-daily (OAD) milking cows	317:359	The objectives of this study were to estimate the genetic and phenotypic correlations and heritabilities for milk production and fertility traits in spring-calved once-daily (OAD) milking cows for the whole season in New Zealand and compare those estimates with twice-daily (TAD) milking cows.
36710178	5	146	theme	milking	997:1003	arg1	populations					1009:1019	both OAD and TAD milking cow populations	980:1019	populations	1009:1019	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36710178	10	147	theme	first	1818:1822	arg1	service					1824:1830	first service	1818:1830	first service to conception	1818:1844	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	4	148	theme	Heritability	818:829	arg1	estimates					831:839	Heritability estimates	818:839	Heritability estimates of production traits	818:860	Heritability estimates of production traits varied from 0.26 to 0.61 in OAD and from 0.13 to 0.63 in TAD.
36710178	10	149	theme	milking	1961:1967	arg1	population					1973:1982	the OAD milking cow population	1953:1982	the OAD milking cow population	1953:1982	Protein percentage was positively genetically correlated with 3-wk and 6-wk submission, 3-wk in-calf, 6-wk in-calf, first service to conception, 3-wk calving, and 6-wk calving rate in the TAD milking cow population, but these correlations were low in the OAD milking cow population.
36710178	8	150	theme	6-wk	1534:1537	arg1	rate					1547:1550	6-wk calving rate	1534:1550	6-wk calving rate	1534:1550	In OAD milking cows, genetic correlations of milk and lactose yields with the start of mating to conception, 6-wk in-calf, not-in-calf, and 6-wk calving rate were close to zero.
36710178	2	151	dep	seasons	540:546	arg1	2017-2018					561:569	2017-2018	561:569	2017-2018	561:569	Data used in the study consisted of 69,252 first parity cows from the calving seasons 2015-2016 to 2017-2018 in 113 OAD and 531 TAD milking herds.
36710178	5	152	dep	consistent	1041:1050	arg1	OAD					1053:1055	OAD	1053:1055	OAD	1053:1055	Heritability estimates for fertility traits were low in both OAD and TAD milking cow populations, and estimates were consistent (OAD: 0.01 to 0.10 and TAD: 0.01 to 0.08) across milking regimens.
36308636	4	0	theme	extracted	912:920	arg1	EC					933:934	EC	933:934	EC	933:934	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	4	0	theme	extracted	912:920	arg1	cellulose					922:930	the extracted cellulose	908:930	the extracted cellulose (EC)	908:935	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	8	1	theme	additives	1654:1662	arg1	method					1680:1685	an effective method	1667:1685	an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass	1667:1793	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	8	1	theme	additives	1654:1662	arg1	addition					1642:1649	The addition	1638:1649	The addition of additives	1638:1662	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	5	2	theme	additives	1272:1280	arg1	addition					1256:1263	the addition	1252:1263	the addition of two additives	1252:1280	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	8	3	theme	enzymatic	1701:1709	arg1	efficiency					1722:1731	enzymatic hydrolysis efficiency	1701:1731	enzymatic hydrolysis efficiency	1701:1731	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	3	4	theme	scanning	641:648	arg1	microscope					659:668	scanning electron microscope	641:668	scanning electron microscope (SEM)	641:674	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	3	4	theme	scanning	641:648	arg1	SEM					671:673	SEM	671:673	SEM	671:673	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	8	5	theme	sugar	1749:1753	arg1	production					1755:1764	fermentable sugar production	1737:1764	fermentable sugar production	1737:1764	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	1	6	theme	wheat	400:404	arg1	WS					413:414	WS	413:414	WS	413:414	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	6	theme	wheat	400:404	arg1	straw					406:410	wheat straw	400:410	wheat straw (WS)	400:415	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	3	7	dep	composition	756:766	arg1	the					752:754	the	752:754	the	752:754	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	2	8	theme	enzymatic	469:477	arg1	model					498:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model was used to successfully describe the enzymatic hydrolysis in a heterogeneous system.
36308636	1	9	theme	additive	293:300	arg1	concentrations					302:315	additive concentrations	293:315	additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	293:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	2	10	theme	enzymatic	542:550	arg1	hydrolysis					552:561	the enzymatic hydrolysis	538:561	the enzymatic hydrolysis	538:561	An enzymatic hydrolysis kinetic model was used to successfully describe the enzymatic hydrolysis in a heterogeneous system.
36308636	5	11	theme	sugar	1081:1085	arg1	concentration					1087:1099	The total reducing sugar concentration	1062:1099	The total reducing sugar concentration with the addition of the TX-100	1062:1131	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	3	12	used	used	681:684	arg2	Fourier					590:596	Fourier	590:596	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)	590:674	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	5	13	theme	total	1066:1070	arg1	concentration					1087:1099	The total reducing sugar concentration	1062:1099	The total reducing sugar concentration with the addition of the TX-100	1062:1131	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	6	14	theme	initial	1383:1389	arg1	rate					1421:1424	the initial enzymatic hydrolysis reaction rate	1379:1424	the initial enzymatic hydrolysis reaction rate (Km) of the EC	1379:1439	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	14	theme	initial	1383:1389	arg1	times					1460:1464	more than four times	1445:1464	more than four times greater than that of the raw	1445:1493	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	14	theme	initial	1383:1389	arg1	Km					1427:1428	Km	1427:1428	Km	1427:1428	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	5	15	theme	BSA	1210:1212	arg1	addition					1194:1201	the addition	1190:1201	the addition of the BSA (5.728 g/L at 120 h)	1190:1233	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	6	16	theme	reaction	1412:1419	arg1	rate					1421:1424	the initial enzymatic hydrolysis reaction rate	1379:1424	the initial enzymatic hydrolysis reaction rate (Km) of the EC	1379:1439	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	16	theme	reaction	1412:1419	arg1	times					1460:1464	more than four times	1445:1464	more than four times greater than that of the raw	1445:1493	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	16	theme	reaction	1412:1419	arg1	Km					1427:1428	Km	1427:1428	Km	1427:1428	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	1	17	from	albumin	258:264	arg1	hydrolysis					330:339	enzymatic hydrolysis	320:339	enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	320:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	5	18	from	120 h	1228:1232	arg1	5.728 g/L					1215:1223	5.728 g/L	1215:1223	5.728 g/L	1215:1223	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	1	19	theme	lignocellulose	156:169	arg1	biomass					171:177	lignocellulose biomass	156:177	lignocellulose biomass	156:177	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	19	theme	lignocellulose	156:169	arg1	effect					184:189	the effect	180:189	the effect of two different additives	180:216	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	20	theme	enzymatic	320:328	arg1	hydrolysis					330:339	enzymatic hydrolysis	320:339	enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	320:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	8	21	theme	effective	1670:1678	arg1	method					1680:1685	an effective method	1667:1685	an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass	1667:1793	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	8	21	theme	effective	1670:1678	arg1	addition					1642:1649	The addition	1638:1649	The addition of additives	1638:1662	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	5	22	with	concentration	1087:1099	arg1	addition					1110:1117	the addition	1106:1117	the addition of the TX-100	1106:1131	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	7	23	theme	EC	1510:1511	arg1	Km					1500:1501	The Km	1496:1501	The Km of the EC added with TX-100 and BSA	1496:1537	The Km of the EC added with TX-100 and BSA were increased by 29.50% and 22.89% compared with that of the EC without the addition of additive.
36308636	1	24	theme	biomass	171:177	arg1	hydrolysis					142:151	enzymatic hydrolysis	132:151	enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives	132:216	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	0	25	theme	Enhancing	10:18	arg1	Hydrolysis					30:39	Enhancing Enzymatic Hydrolysis	10:39	Enhancing Enzymatic Hydrolysis of Wheat Straw	10:54	Additives Enhancing Enzymatic Hydrolysis of Wheat Straw to Obtain Fermentable Sugar.
36308636	2	26	theme	heterogeneous	568:580	arg1	system					582:587	a heterogeneous system	566:587	a heterogeneous system	566:587	An enzymatic hydrolysis kinetic model was used to successfully describe the enzymatic hydrolysis in a heterogeneous system.
36308636	5	27	theme	reducing	1072:1079	arg1	concentration					1087:1099	The total reducing sugar concentration	1062:1099	The total reducing sugar concentration with the addition of the TX-100	1062:1131	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	4	28	theme	reducing	832:839	arg1	concentration					847:859	the total reducing sugar concentration	822:859	the total reducing sugar concentration of the raw	822:870	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	3	29	theme	electron	650:657	arg1	microscope					659:668	scanning electron microscope	641:668	scanning electron microscope (SEM)	641:674	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	3	29	theme	electron	650:657	arg1	SEM					671:673	SEM	671:673	SEM	671:673	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	1	30	theme	Bovine	245:250	arg1	albumin					258:264	Bovine serum albumin	245:264	Bovine serum albumin (BSA)	245:270	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	30	theme	Bovine	245:250	arg1	BSA					267:269	BSA	267:269	BSA	267:269	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	3	31	from	effect	703:708	arg1	structure					772:780	structure	772:780	structure	772:780	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	3	31	from	effect	703:708	arg1	composition					756:766	composition	756:766	composition	756:766	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	6	32	theme	greater	1466:1472	arg1	rate					1421:1424	the initial enzymatic hydrolysis reaction rate	1379:1424	the initial enzymatic hydrolysis reaction rate (Km) of the EC	1379:1439	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	32	theme	greater	1466:1472	arg1	times					1460:1464	more than four times	1445:1464	more than four times greater than that of the raw	1445:1493	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	7	33	dep	increased	1544:1552	arg1	compared					1575:1582	compared	1575:1582	compared with that of the EC without the addition of additive	1575:1635	The Km of the EC added with TX-100 and BSA were increased by 29.50% and 22.89% compared with that of the EC without the addition of additive.
36308636	6	34	theme	hydrolysis	1401:1410	arg1	rate					1421:1424	the initial enzymatic hydrolysis reaction rate	1379:1424	the initial enzymatic hydrolysis reaction rate (Km) of the EC	1379:1439	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	34	theme	hydrolysis	1401:1410	arg1	times					1460:1464	more than four times	1445:1464	more than four times greater than that of the raw	1445:1493	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	34	theme	hydrolysis	1401:1410	arg1	Km					1427:1428	Km	1427:1428	Km	1427:1428	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	1	35	from	concentrations	302:315	arg1	hydrolysis					330:339	enzymatic hydrolysis	320:339	enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	320:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	36	theme	serum	252:256	arg1	albumin					258:264	Bovine serum albumin	245:264	Bovine serum albumin (BSA)	245:270	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	36	theme	serum	252:256	arg1	BSA					267:269	BSA	267:269	BSA	267:269	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	4	37	theme	raw	868:870	arg1	concentration					847:859	the total reducing sugar concentration	822:859	the total reducing sugar concentration of the raw	822:870	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	1	38	from	effect	109:114	arg1	hydrolysis					142:151	enzymatic hydrolysis	132:151	enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives	132:216	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	5	39	theme	enzymatic	1295:1303	arg1	efficiency					1316:1325	the enzymatic hydrolysis efficiency	1291:1325	the enzymatic hydrolysis efficiency	1291:1325	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	0	40	theme	Enzymatic	20:28	arg1	Hydrolysis					30:39	Enhancing Enzymatic Hydrolysis	10:39	Enhancing Enzymatic Hydrolysis of Wheat Straw	10:54	Additives Enhancing Enzymatic Hydrolysis of Wheat Straw to Obtain Fermentable Sugar.
36308636	7	41	theme	additive	1628:1635	arg1	addition					1616:1623	the addition	1612:1623	the addition of additive	1612:1635	The Km of the EC added with TX-100 and BSA were increased by 29.50% and 22.89% compared with that of the EC without the addition of additive.
36308636	4	42	theme	enzymatic	1018:1026	arg1	hydrolysis					1028:1037	enzymatic hydrolysis	1018:1037	enzymatic hydrolysis	1018:1037	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	0	43	theme	Wheat	44:48	arg1	Straw					50:54	Wheat Straw	44:54	Wheat Straw	44:54	Additives Enhancing Enzymatic Hydrolysis of Wheat Straw to Obtain Fermentable Sugar.
36308636	4	44	from	WS	994:995	arg1	EC					986:987	EC	986:987	EC from WS	986:995	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	2	45	theme	kinetic	490:496	arg1	model					498:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model was used to successfully describe the enzymatic hydrolysis in a heterogeneous system.
36308636	5	46	theme	TX-100	1126:1131	arg1	addition					1110:1117	the addition	1106:1117	the addition of the TX-100	1106:1131	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	8	47	theme	hydrolysis	1711:1720	arg1	efficiency					1722:1731	enzymatic hydrolysis efficiency	1701:1731	enzymatic hydrolysis efficiency	1701:1731	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	8	48	theme	fermentable	1737:1747	arg1	production					1755:1764	fermentable sugar production	1737:1764	fermentable sugar production	1737:1764	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	2	49	theme	hydrolysis	479:488	arg1	model					498:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model was used to successfully describe the enzymatic hydrolysis in a heterogeneous system.
36308636	1	50	theme	fermentable	351:361	arg1	sugar					363:367	fermentable sugar	351:367	fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	351:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	3	51	theme	hydrolysis	738:747	arg1	effect					703:708	the effect	699:708	the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples	699:795	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	1	52	theme	different	198:206	arg1	additives					208:216	two different additives	194:216	two different additives	194:216	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	53	from	dosages	280:286	arg1	hydrolysis					330:339	enzymatic hydrolysis	320:339	enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	320:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	54	dep	hydrolysis	330:339	arg1	obtain					344:349	obtain	344:349	to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	341:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	4	55	theme	sugar	841:845	arg1	concentration					847:859	the total reducing sugar concentration	822:859	the total reducing sugar concentration of the raw	822:870	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	1	56	dep	explore	97:103	arg1	to					94:95	to	94:95	to	94:95	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	0	57	theme	Straw	50:54	arg1	Hydrolysis					30:39	Enhancing Enzymatic Hydrolysis	10:39	Enhancing Enzymatic Hydrolysis of Wheat Straw	10:54	Additives Enhancing Enzymatic Hydrolysis of Wheat Straw to Obtain Fermentable Sugar.
36308636	1	58	from	X-100	226:230	arg1	hydrolysis					330:339	enzymatic hydrolysis	320:339	enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate	320:432	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	5	59	dep	BSA	1210:1212	arg1	5.728 g/L					1215:1223	5.728 g/L	1215:1223	5.728 g/L	1215:1223	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	4	60	theme	total	826:830	arg1	concentration					847:859	the total reducing sugar concentration	822:859	the total reducing sugar concentration of the raw	822:870	The results showed that the total reducing sugar concentration of the raw was 1.535 g/L at 120 h, but that of the extracted cellulose (EC) increased to 5.087 g/L at 120 h, indicating that EC from WS is more conducive to enzymatic hydrolysis compared with the raw.
36308636	1	61	theme	enzyme	273:278	arg1	dosages					280:286	enzyme dosages	273:286	enzyme dosages	273:286	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	6	62	theme	enzymatic	1391:1399	arg1	rate					1421:1424	the initial enzymatic hydrolysis reaction rate	1379:1424	the initial enzymatic hydrolysis reaction rate (Km) of the EC	1379:1439	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	62	theme	enzymatic	1391:1399	arg1	times					1460:1464	more than four times	1445:1464	more than four times greater than that of the raw	1445:1493	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	62	theme	enzymatic	1391:1399	arg1	Km					1427:1428	Km	1427:1428	Km	1427:1428	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	5	63	theme	hydrolysis	1305:1314	arg1	efficiency					1316:1325	the enzymatic hydrolysis efficiency	1291:1325	the enzymatic hydrolysis efficiency	1291:1325	The total reducing sugar concentration with the addition of the TX-100 was 6.737 g/L at 120 h, which was greater than that with the addition of the BSA (5.728 g/L at 120 h), indicating that the addition of two additives improved the enzymatic hydrolysis efficiency, especially TX-100.
36308636	3	64	dep	Fourier	590:596	arg1	transform					598:606	transform	598:606	transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)	598:674	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	1	65	theme	additives	208:216	arg1	biomass					171:177	lignocellulose biomass	156:177	lignocellulose biomass	156:177	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	65	theme	additives	208:216	arg1	effect					184:189	the effect	180:189	the effect of two different additives	180:216	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	3	66	theme	extraction	713:722	arg1	effect					703:708	the effect	699:708	the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples	699:795	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	6	67	theme	EC	1438:1439	arg1	rate					1421:1424	the initial enzymatic hydrolysis reaction rate	1379:1424	the initial enzymatic hydrolysis reaction rate (Km) of the EC	1379:1439	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	67	theme	EC	1438:1439	arg1	times					1460:1464	more than four times	1445:1464	more than four times greater than that of the raw	1445:1493	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	6	67	theme	EC	1438:1439	arg1	Km					1427:1428	Km	1427:1428	Km	1427:1428	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	1	68	theme	additives	119:127	arg1	effect					109:114	the effect	105:114	the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives	105:216	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	3	69	theme	samples	789:795	arg1	structure					772:780	structure	772:780	structure	772:780	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	3	69	theme	samples	789:795	arg1	composition					756:766	composition	756:766	composition	756:766	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	3	70	dep	transform	598:606	arg1	infrared					608:615	infrared	608:615	transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM)	598:674	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36308636	0	71	theme	Fermentable	66:76	arg1	Sugar					78:82	Fermentable Sugar	66:82	Fermentable Sugar	66:82	Additives Enhancing Enzymatic Hydrolysis of Wheat Straw to Obtain Fermentable Sugar.
36308636	2	72	used	used	508:511	arg2	model					498:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model	466:502	An enzymatic hydrolysis kinetic model was used to successfully describe the enzymatic hydrolysis in a heterogeneous system.
36308636	6	73	theme	kinetic	1351:1357	arg1	studies					1359:1365	The kinetic studies	1347:1365	The kinetic studies	1347:1365	The kinetic studies showed that the initial enzymatic hydrolysis reaction rate (Km) of the EC was more than four times greater than that of the raw.
36308636	1	74	theme	Triton	219:224	arg1	TX-100					233:238	TX-100	233:238	TX-100	233:238	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	1	74	theme	Triton	219:224	arg1	X-100					226:230	Triton X-100	219:230	Triton X-100 (TX-100)	219:239	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	8	75	theme	lignocellulosic	1771:1785	arg1	biomass					1787:1793	lignocellulosic biomass	1771:1793	lignocellulosic biomass	1771:1793	The addition of additives is an effective method for enhancing enzymatic hydrolysis efficiency and fermentable sugar production from lignocellulosic biomass.
36308636	1	76	theme	enzymatic	132:140	arg1	hydrolysis					142:151	enzymatic hydrolysis	132:151	enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives	132:216	In order to explore the effect of additives on enzymatic hydrolysis of lignocellulose biomass, the effect of two different additives, Triton X-100 (TX-100) and Bovine serum albumin (BSA), enzyme dosages, and additive concentrations on enzymatic hydrolysis to obtain fermentable sugar using cellulose extracted from wheat straw (WS) as the substrate was investigated in this study.
36308636	3	77	theme	enzymatic	728:736	arg1	hydrolysis					738:747	enzymatic hydrolysis	728:747	enzymatic hydrolysis	728:747	Fourier transform infrared spectroscopy (FTIR) and scanning electron microscope (SEM) were used to determine the effect of extraction and enzymatic hydrolysis on the composition and structure of the samples.
36152776	4	0	theme	had little	904:913	arg1	ability					915:921	had little ability	904:921	had little ability to change circulating LPS level and cross the blood-brain barrier	904:987	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	0	1	from	effects	15:21	arg1	D-galactose/AlCl3					63:79	D-galactose/AlCl3	63:79	D-galactose/AlCl3	63:79	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats.
36152776	2	2	theme	biochemical	543:553	arg1	tests					555:559	biochemical tests	543:559	biochemical tests	543:559	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	6	3	contain	has	1366:1368	arg1	muciniphila					1354:1364	A. muciniphila	1351:1364	A. muciniphila	1351:1364	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	6	3	contain	has	1366:1368	arg2	effect					1382:1387	a remissive effect	1370:1387	a remissive effect	1370:1387	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	2	4	theme	Morris	341:346	arg1	test					359:362	Morris water maze test	341:362	Morris water maze test	341:362	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	5	5	theme	neurotransmitter-producing	1103:1128	arg1	microbiome					1134:1143	neurotransmitter-producing gut microbiome	1103:1143	neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus	1103:1175	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	2	6	used	used	336:339	arg2	We					333:334	We	333:334	We	333:334	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	5	7	theme	gut	1130:1132	arg1	microbiome					1134:1143	neurotransmitter-producing gut microbiome	1103:1143	neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus	1103:1175	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	3	8	theme	KEY	621:623	arg1	FINDINGS					625:632	KEY FINDINGS	621:632	KEY FINDINGS We found that A. muciniphila could alleviate AD-like rats' cognitive impairment and mitigate ligature-induced periodontitis.	621:757	KEY FINDINGS We found that A. muciniphila could alleviate AD-like rats' cognitive impairment and mitigate ligature-induced periodontitis.
36152776	6	9	theme	community	1513:1521	arg1	composition					1471:1481	composition	1471:1481	composition	1471:1481	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	6	9	theme	community	1513:1521	arg1	function					1487:1494	function	1487:1494	function	1487:1494	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	1	10	theme	muciniphila	245:255	arg1	treatment					229:237	an oral treatment	221:237	an oral treatment of A. muciniphila	221:255	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	0	11	theme	disease-like	102:113	arg1	rats					115:118	Alzheimer's disease-like rats	90:118	Alzheimer's disease-like rats	90:118	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats.
36152776	2	12	theme	LAL	530:532	arg1	Test					534:537	LAL Test	530:537	LAL Test	530:537	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	6	13	theme	microbial	1503:1511	arg1	community					1513:1521	gut microbial community	1499:1521	gut microbial community	1499:1521	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	4	14	from	deposition	802:811	arg1	cortex					820:825	cortex	820:825	cortex	820:825	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	4	14	from	deposition	802:811	arg1	regions					831:837	regions	831:837	regions	831:837	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	2	15	theme	gut	566:568	arg1	microbiota					570:579	The gut microbiota	562:579	The gut microbiota	562:579	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	2	16	theme	cognitive	391:399	arg1	impairment					401:410	the cognitive impairment	387:410	the cognitive impairment	387:410	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	6	17	theme	gut	1499:1501	arg1	community					1513:1521	gut microbial community	1499:1521	gut microbial community	1499:1521	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	2	18	theme	LPS	470:472	arg1	level					474:478	Serum LPS level	464:478	Serum LPS level	464:478	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	5	19	theme	acid	1078:1081	arg1	abundance					1042:1050	the abundance	1038:1050	the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus	1038:1191	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	0	20	theme	preventive	4:13	arg1	effects					15:21	The preventive effects	0:21	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3	0:79	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats.
36152776	6	21	dep	suggested	1336:1344	arg1	SIGNIFICANCE					1310:1321	SIGNIFICANCE	1310:1321	SIGNIFICANCE	1310:1321	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	2	22	theme	gene	606:609	arg1	sequence					611:618	16S rRNA gene sequence	597:618	16S rRNA gene sequence	597:618	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	4	23	theme	LPS	945:947	arg1	level					949:953	circulating LPS level	933:953	circulating LPS level	933:953	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	1	24	theme	AD-like	260:266	arg1	rats					268:271	AD-like rats	260:271	AD-like rats with periodontitis and its possible mechanism	260:317	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	3	25	theme	AD-like	679:685	arg1	impairment					703:712	AD-like rats' cognitive impairment	679:712	AD-like rats' cognitive impairment	679:712	KEY FINDINGS We found that A. muciniphila could alleviate AD-like rats' cognitive impairment and mitigate ligature-induced periodontitis.
36152776	2	26	theme	rRNA	601:604	arg1	sequence					611:618	16S rRNA gene sequence	597:618	16S rRNA gene sequence	597:618	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	4	27	theme	Aβ1-42	795:800	arg1	deposition					802:811	Aβ1-42 deposition	795:811	Aβ1-42 deposition in the cortex and regions of the rats' brain	795:856	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	5	28	theme	Aerococcus	1228:1237	arg1	abundance					1204:1212	the abundance	1200:1212	the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation,	1200:1292	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	2	29	theme	bone	416:419	arg1	loss					421:424	bone loss	416:424	bone loss	416:424	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	2	30	theme	water	348:352	arg1	test					359:362	Morris water maze test	341:362	Morris water maze test	341:362	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	2	31	theme	AST/ALT	498:504	arg1	levels					506:511	AST/ALT levels	498:511	AST/ALT levels	498:511	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	1	32	with	rats	268:271	arg1	periodontitis					278:290	periodontitis	278:290	periodontitis	278:290	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	1	32	with	rats	268:271	arg1	mechanism					309:317	its possible mechanism	296:317	its possible mechanism	296:317	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	3	33	dep	FINDINGS	625:632	arg1	found					637:641	found	637:641	found that A. muciniphila could alleviate AD-like rats' cognitive impairment and mitigate ligature-induced periodontitis	637:756	KEY FINDINGS We found that A. muciniphila could alleviate AD-like rats' cognitive impairment and mitigate ligature-induced periodontitis.
36152776	4	34	theme	brain	852:856	arg1	cortex					820:825	cortex	820:825	cortex	820:825	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	4	34	theme	brain	852:856	arg1	regions					831:837	regions	831:837	regions	831:837	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	2	35	dep	METHODS	325:331	arg1	used					336:339	used	336:339	used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss	336:424	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	2	35	dep	METHODS	325:331	arg1	tested					449:454	tested	449:454	was tested by IHC	445:461	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	5	36	theme	Streptococcus	1243:1255	arg1	abundance					1204:1212	the abundance	1200:1212	the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation,	1200:1292	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	2	37	theme	maze	354:357	arg1	test					359:362	Morris water maze test	341:362	Morris water maze test	341:362	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	0	38	theme	Akkermansia	36:46	arg1	muciniphila					48:58	probiotic Akkermansia muciniphila	26:58	probiotic Akkermansia muciniphila	26:58	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats.
36152776	0	39	theme	probiotic	26:34	arg1	muciniphila					48:58	probiotic Akkermansia muciniphila	26:58	probiotic Akkermansia muciniphila	26:58	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats.
36152776	5	40	theme	chain	1066:1070	arg1	acid					1078:1081	some short chain fatty acid	1055:1081	some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus	1055:1191	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	5	40	theme	chain	1066:1070	arg1	SCFA					1084:1087	SCFA	1084:1087	SCFA	1084:1087	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	5	41	theme	A.	999:1000	arg1	treatment					1014:1022	A. muciniphila treatment	999:1022	A. muciniphila treatment	999:1022	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	6	42	theme	remissive	1372:1380	arg1	effect					1382:1387	a remissive effect	1370:1387	a remissive effect	1370:1387	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	3	43	theme	cognitive	693:701	arg1	impairment					703:712	AD-like rats' cognitive impairment	679:712	AD-like rats' cognitive impairment	679:712	KEY FINDINGS We found that A. muciniphila could alleviate AD-like rats' cognitive impairment and mitigate ligature-induced periodontitis.
36152776	4	44	theme	circulating	933:943	arg1	level					949:953	circulating LPS level	933:953	circulating LPS level	933:953	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	5	45	theme	fatty	1072:1076	arg1	acid					1078:1081	some short chain fatty acid	1055:1081	some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus	1055:1191	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	5	45	theme	fatty	1072:1076	arg1	SCFA					1084:1087	SCFA	1084:1087	SCFA	1084:1087	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	5	46	theme	muciniphila	1002:1012	arg1	treatment					1014:1022	A. muciniphila treatment	999:1022	A. muciniphila treatment	999:1022	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	1	47	from	effects	210:216	arg1	rats					268:271	AD-like rats	260:271	AD-like rats with periodontitis and its possible mechanism	260:317	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	0	48	theme	muciniphila	48:58	arg1	effects					15:21	The preventive effects	0:21	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3	0:79	The preventive effects of probiotic Akkermansia muciniphila on D-galactose/AlCl3 mediated Alzheimer's disease-like rats.
36152776	1	49	theme	possible	300:307	arg1	mechanism					309:317	its possible mechanism	296:317	its possible mechanism	296:317	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	4	50	dep	A.	772:773	arg1	muciniphila					775:785	A. muciniphila	772:785	A. muciniphila	772:785	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	2	51	theme	Aβ1-42	427:432	arg1	deposition					434:443	Aβ1-42 deposition	427:443	Aβ1-42 deposition	427:443	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	2	52	theme	16S	597:599	arg1	rRNA					601:604	16S rRNA	597:604	16S rRNA gene sequence	597:618	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	6	53	theme	circulation	1548:1558	arg1	metabolism					1560:1569	peripheral circulation metabolism	1537:1569	peripheral circulation metabolism	1537:1569	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	2	54	theme	MAIN	320:323	arg1	METHODS					325:331	MAIN METHODS	320:331	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.	320:619	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	6	55	theme	gut-brain	1439:1447	arg1	axis					1449:1452	gut-brain axis	1439:1452	gut-brain axis	1439:1452	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	6	56	theme	peripheral	1537:1546	arg1	metabolism					1560:1569	peripheral circulation metabolism	1537:1569	peripheral circulation metabolism	1537:1569	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	1	57	theme	AD-like	141:147	arg1	models					153:158	the AD-like rat models	137:158	the AD-like rat models injected by AlCl3 and D-galactose	137:192	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	2	58	theme	Serum	464:468	arg1	level					474:478	Serum LPS level	464:478	Serum LPS level	464:478	MAIN METHODS We used Morris water maze test and micro-CT to assess the cognitive impairment and bone loss; Aβ1-42 deposition was tested by IHC; Serum LPS level and TG, HDL-C and AST/ALT levels were detected by LAL Test and biochemical tests; The gut microbiota was analyzed by 16S rRNA gene sequence.
36152776	6	59	theme	AD-like	1392:1398	arg1	pathologies					1400:1410	AD-like pathologies	1392:1410	AD-like pathologies	1392:1410	SIGNIFICANCE Our findings suggested that A. muciniphila has a remissive effect on AD-like pathologies, potentially by regulating gut-brain axis through altering composition and function of gut microbial community or moderating peripheral circulation metabolism.
36152776	5	60	theme	short	1060:1064	arg1	acid					1078:1081	some short chain fatty acid	1055:1081	some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus	1055:1191	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	5	60	theme	short	1060:1064	arg1	SCFA					1084:1087	SCFA	1084:1087	SCFA	1084:1087	Notably, A. muciniphila treatment could improve the abundance of some short chain fatty acid (SCFA)-producing or neurotransmitter-producing gut microbiome such as Blautia, Staphylococcus and Lactococcus, while the abundance of pathogenic Aerococcus and Streptococcus, which were associated inflammation, were decreased.
36152776	1	61	theme	rat	149:151	arg1	models					153:158	the AD-like rat models	137:158	the AD-like rat models injected by AlCl3 and D-galactose	137:192	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	1	62	theme	oral	224:227	arg1	treatment					229:237	an oral treatment	221:237	an oral treatment of A. muciniphila	221:255	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	4	63	theme	AST/ALT	885:891	arg1	levels					893:898	AST/ALT levels	885:898	AST/ALT levels	885:898	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	3	64	theme	ligature-induced	727:742	arg1	periodontitis					744:756	ligature-induced periodontitis	727:756	ligature-induced periodontitis	727:756	KEY FINDINGS We found that A. muciniphila could alleviate AD-like rats' cognitive impairment and mitigate ligature-induced periodontitis.
36152776	4	65	dep	cortex	820:825	arg1	the					816:818	the	816:818	the	816:818	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	1	66	dep	AIMS	121:124	arg1	induced					129:135	induced	129:135	induced	129:135	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
36152776	4	67	theme	blood-brain	969:979	arg1	barrier					981:987	the blood-brain barrier	965:987	the blood-brain barrier	965:987	Furthermore, A. muciniphila reduced Aβ1-42 deposition in the cortex and regions of the rats' brain, and altered TG, HDL-C and AST/ALT levels but had little ability to change circulating LPS level and cross the blood-brain barrier.
36152776	1	68	theme	treatment	229:237	arg1	effects					210:216	the effects	206:216	the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism	206:317	AIMS We induced the AD-like rat models injected by AlCl3 and D-galactose, to explore the effects of an oral treatment of A. muciniphila on AD-like rats with periodontitis and its possible mechanism.
35196163	3	0	theme	dermal	655:660	arg1	HDF					675:677	HDF	675:677	HDF	675:677	In validation, TSP1 and PDIA3 were established to bind in vitro and to colocalize in the endoplasmic reticulum of human dermal fibroblasts (HDF).
35196163	3	0	theme	dermal	655:660	arg1	fibroblasts					662:672	human dermal fibroblasts	649:672	human dermal fibroblasts (HDF)	649:678	In validation, TSP1 and PDIA3 were established to bind in vitro and to colocalize in the endoplasmic reticulum of human dermal fibroblasts (HDF).
35196163	5	1	theme	extracellular	1135:1147	arg1	matrix					1149:1154	extracellular matrix	1135:1154	extracellular matrix (WTECM)	1135:1162	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	5	1	theme	extracellular	1135:1147	arg1	WTECM					1157:1161	WTECM	1157:1161	WTECM	1157:1161	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	8	2	theme	Pdia3	1582:1586	arg1	MEFs					1588:1591	Pdia3 MEFs	1582:1591	Pdia3 MEFs	1582:1591	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	9	3	from	MEFs	1724:1727	arg1	activity					1698:1705	the adhesion-promoting activity	1675:1705	the adhesion-promoting activity of WTCM on Pdia3 MEFs	1675:1727	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	9	4	theme	communication	1609:1621	arg1	CCN2					1634:1637	CCN2	1634:1637	CCN2	1634:1637	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	9	4	theme	communication	1609:1621	arg1	network					1623:1629	cell communication network 2	1604:1631	cell communication network 2 (CCN2)	1604:1638	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	4	5	theme	matrix	868:873	arg1	composition					826:836	the composition	822:836	the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1,	822:927	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	5	6	dep	wild-type	1169:1177	arg1	WT					1180:1181	WT	1180:1181	WT	1180:1181	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	1	7	theme	cell	209:212	arg1	surfaces					214:221	cell surfaces	209:221	cell surfaces	209:221	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	8	8	theme	quantitative	1376:1387	arg1	proteomics					1405:1414	Comparative quantitative tandem-mass-tag proteomics	1364:1414	Comparative quantitative tandem-mass-tag proteomics	1364:1414	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	4	9	theme	cell-derived	841:852	arg1	matrix					868:873	cell-derived extracellular matrix	841:873	cell-derived extracellular matrix	841:873	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	6	10	theme	PDIA3	1209:1213	arg1	activity					1215:1222	PDIA3 activity	1209:1222	PDIA3 activity in WT-MEFs	1209:1233	Rescue depended on PDIA3 activity in WT-MEFs and was not prevented by immunodepletion of fibronectin.
35196163	4	11	theme	TSP1	923:926	arg1	abundance					894:902	changed abundance	886:902	changed abundance of fibronectin and TSP1	886:926	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	8	12	theme	functional	1420:1429	arg1	assays					1431:1436	functional assays	1420:1436	functional assays	1420:1436	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	5	13	theme	Pdia3	1064:1068	arg1	MEFs					1070:1073	Pdia3 MEFs	1064:1073	Pdia3 MEFs	1064:1073	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	4	14	theme	altered	973:979	arg1	organization					981:992	altered organization	973:992	altered organization of F-actin	973:1003	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	8	15	dep	MEFs	1588:1591	arg1	CM					1576:1577	CM	1576:1577	CM	1576:1577	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	8	15	dep	MEFs	1588:1591	arg1	the					1572:1574	the	1572:1574	the	1572:1574	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	2	16	theme	candidate	508:516	arg1	protein					526:532	a novel candidate binding protein	500:532	a novel candidate binding protein	500:532	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	16	theme	candidate	508:516	arg1	isomerase					470:478	protein disulfide isomerase A3	452:481	protein disulfide isomerase A3 (PDIA3/ERp57)	452:495	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	8	17	theme	heparin-binding	1445:1459	arg1	secretomes					1461:1470	the heparin-binding secretomes	1441:1470	the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs	1441:1496	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	4	18	theme	pharmacological	715:729	arg1	inhibition					731:740	pharmacological inhibition	715:740	pharmacological inhibition in HDF	715:747	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	4	19	theme	fibronectin	907:917	arg1	abundance					894:902	changed abundance	886:902	changed abundance of fibronectin and TSP1	886:926	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	1	20	gly	glycoprotein	113:124	arg1	glycoprotein					113:124	The matricellular glycoprotein thrombospondin-1	95:141	The matricellular glycoprotein thrombospondin-1 (TSP1)	95:148	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	9	21	theme	Pdia3	1718:1722	arg1	MEFs					1724:1727	Pdia3 MEFs	1718:1727	Pdia3 MEFs	1718:1727	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	4	22	theme	function	695:702	arg1	Loss					681:684	Loss	681:684	Loss of PDIA3 function	681:702	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	1	23	theme	matricellular	99:111	arg1	TSP1					144:147	TSP1	144:147	TSP1	144:147	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	1	23	theme	matricellular	99:111	arg1	thrombospondin-1					126:141	The matricellular glycoprotein thrombospondin-1	95:141	The matricellular glycoprotein thrombospondin-1 (TSP1)	95:148	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	2	24	theme	interactome	418:428	arg1	screen					430:435	a biotin ligase-based TSP1 interactome screen	391:435	a biotin ligase-based TSP1 interactome screen	391:435	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	8	25	theme	MEFs	1493:1496	arg1	secretomes					1461:1470	the heparin-binding secretomes	1441:1470	the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs	1441:1496	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	2	26	theme	ligase-based	400:411	arg1	screen					430:435	a biotin ligase-based TSP1 interactome screen	391:435	a biotin ligase-based TSP1 interactome screen	391:435	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	9	27	from	WTCM	1710:1713	arg1	MEFs					1724:1727	Pdia3 MEFs	1718:1727	Pdia3 MEFs	1718:1727	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	7	28	from	proteins	1308:1315	arg1	WTCM					1320:1323	WTCM	1320:1323	WTCM	1320:1323	Heparin-binding proteins in WTCM were found to be necessary for rescue.
35196163	8	29	theme	multiple	1509:1516	arg1	ECM					1518:1520	multiple ECM and growth factor proteins	1509:1547	ECM	1518:1520	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	4	30	theme	embryo	767:772	arg1	fibroblasts					774:784	Pdia3 mouse embryo fibroblasts	755:784	Pdia3 mouse embryo fibroblasts (Pdia3 MEFs)	755:797	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	4	30	theme	embryo	767:772	arg1	MEFs					793:796	Pdia3 MEFs	787:796	Pdia3 MEFs	787:796	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	6	31	from	activity	1215:1222	arg1	WT-MEFs					1227:1233	WT-MEFs	1227:1233	WT-MEFs	1227:1233	Rescue depended on PDIA3 activity in WT-MEFs and was not prevented by immunodepletion of fibronectin.
35196163	9	32	from	activity	1698:1705	arg1	MEFs					1724:1727	Pdia3 MEFs	1718:1727	Pdia3 MEFs	1718:1727	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	4	33	theme	cell	957:960	arg1	spreading					962:970	reduced cell spreading	949:970	reduced cell spreading	949:970	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	2	34	theme	intracellular	332:344	arg1	interactions					346:357	novel intracellular interactions	326:357	novel intracellular interactions of TSP1	326:365	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	9	35	theme	cell	1604:1607	arg1	CCN2					1634:1637	CCN2	1634:1637	CCN2	1634:1637	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	9	35	theme	cell	1604:1607	arg1	network					1623:1629	cell communication network 2	1604:1631	cell communication network 2 (CCN2)	1604:1638	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	4	36	theme	focal	1018:1022	arg1	adhesions					1024:1032	reduced focal adhesions	1010:1032	reduced focal adhesions	1010:1032	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	8	37	theme	factor	1533:1538	arg1	proteins					1540:1547	multiple ECM and growth factor proteins	1509:1547	proteins	1540:1547	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	3	38	theme	human	649:653	arg1	HDF					675:677	HDF	675:677	HDF	675:677	In validation, TSP1 and PDIA3 were established to bind in vitro and to colocalize in the endoplasmic reticulum of human dermal fibroblasts (HDF).
35196163	3	38	theme	human	649:653	arg1	fibroblasts					662:672	human dermal fibroblasts	649:672	human dermal fibroblasts (HDF)	649:678	In validation, TSP1 and PDIA3 were established to bind in vitro and to colocalize in the endoplasmic reticulum of human dermal fibroblasts (HDF).
35196163	4	39	theme	Pdia3	755:759	arg1	fibroblasts					774:784	Pdia3 mouse embryo fibroblasts	755:784	Pdia3 mouse embryo fibroblasts (Pdia3 MEFs)	755:797	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	4	39	theme	Pdia3	755:759	arg1	MEFs					793:796	Pdia3 MEFs	787:796	Pdia3 MEFs	787:796	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	2	40	theme	protein	452:458	arg1	PDIA3/ERp57					484:494	PDIA3/ERp57	484:494	PDIA3/ERp57	484:494	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	40	theme	protein	452:458	arg1	protein					526:532	a novel candidate binding protein	500:532	a novel candidate binding protein	500:532	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	40	theme	protein	452:458	arg1	isomerase					470:478	protein disulfide isomerase A3	452:481	protein disulfide isomerase A3 (PDIA3/ERp57)	452:495	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	4	41	theme	F-actin	997:1003	arg1	adhesions					1024:1032	reduced focal adhesions	1010:1032	reduced focal adhesions	1010:1032	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	4	41	theme	F-actin	997:1003	arg1	spreading					962:970	reduced cell spreading	949:970	reduced cell spreading	949:970	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	4	41	theme	F-actin	997:1003	arg1	organization					981:992	altered organization	973:992	altered organization of F-actin	973:1003	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	10	42	theme	microenvironment	1821:1836	arg1	target					1886:1891	a translational target	1870:1891	a translational target	1870:1891	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	10	42	theme	microenvironment	1821:1836	arg1	production					1782:1791	fibroblast production	1771:1791	fibroblast production of an ECM-rich, proadhesive microenvironment	1771:1836	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	4	43	link	cell-derived	841:852	arg1	matrix					868:873	cell-derived extracellular matrix	841:873	cell-derived extracellular matrix	841:873	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	3	44	theme	fibroblasts	662:672	arg1	reticulum					636:644	the endoplasmic reticulum	620:644	the endoplasmic reticulum of human dermal fibroblasts (HDF)	620:678	In validation, TSP1 and PDIA3 were established to bind in vitro and to colocalize in the endoplasmic reticulum of human dermal fibroblasts (HDF).
35196163	5	45	from	-MEFs	1183:1187	arg1	exposure					1094:1101	exposure	1094:1101	exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs	1094:1187	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	1	46	theme	extracellular	175:187	arg1	ECM					197:199	ECM	197:199	ECM	197:199	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	1	46	theme	extracellular	175:187	arg1	matrix					189:194	the extracellular matrix	171:194	the extracellular matrix (ECM)	171:200	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	4	47	theme	Pdia3	787:791	arg1	fibroblasts					774:784	Pdia3 mouse embryo fibroblasts	755:784	Pdia3 mouse embryo fibroblasts (Pdia3 MEFs)	755:797	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	4	47	theme	Pdia3	787:791	arg1	MEFs					793:796	Pdia3 MEFs	787:796	Pdia3 MEFs	787:796	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	10	48	theme	translational	1872:1884	arg1	target					1886:1891	a translational target	1870:1891	a translational target	1870:1891	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	10	48	theme	translational	1872:1884	arg1	production					1782:1791	fibroblast production	1771:1791	fibroblast production of an ECM-rich, proadhesive microenvironment	1771:1836	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	4	49	theme	extracellular	854:866	arg1	matrix					868:873	cell-derived extracellular matrix	841:873	cell-derived extracellular matrix	841:873	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	8	50	theme	Comparative	1364:1374	arg1	proteomics					1405:1414	Comparative quantitative tandem-mass-tag proteomics	1364:1414	Comparative quantitative tandem-mass-tag proteomics	1364:1414	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	2	51	theme	binding	518:524	arg1	protein					526:532	a novel candidate binding protein	500:532	a novel candidate binding protein	500:532	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	51	theme	binding	518:524	arg1	isomerase					470:478	protein disulfide isomerase A3	452:481	protein disulfide isomerase A3 (PDIA3/ERp57)	452:495	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	8	52	theme	tandem-mass-tag	1389:1403	arg1	proteomics					1405:1414	Comparative quantitative tandem-mass-tag proteomics	1364:1414	Comparative quantitative tandem-mass-tag proteomics	1364:1414	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	8	53	from	assays	1431:1436	arg1	secretomes					1461:1470	the heparin-binding secretomes	1441:1470	the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs	1441:1496	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	2	54	contain	carried	379:385	arg1	we					376:377	we	376:377	we	376:377	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	54	contain	carried	379:385	arg2	screen					430:435	a biotin ligase-based TSP1 interactome screen	391:435	a biotin ligase-based TSP1 interactome screen	391:435	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	7	55	theme	Heparin-binding	1292:1306	arg1	proteins					1308:1315	Heparin-binding proteins	1292:1315	Heparin-binding proteins in WTCM	1292:1323	Heparin-binding proteins in WTCM were found to be necessary for rescue.
35196163	1	56	theme	complex	154:160	arg1	roles					162:166	complex roles	154:166	complex roles	154:166	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	4	57	theme	changed	886:892	arg1	abundance					894:902	changed abundance	886:902	changed abundance of fibronectin and TSP1	886:926	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	5	58	theme	MEFs	1070:1073	arg1	phenotypes					1050:1059	These cellular phenotypes	1035:1059	These cellular phenotypes of Pdia3 MEFs	1035:1073	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	9	59	theme	adhesion-promoting	1679:1696	arg1	activity					1698:1705	the adhesion-promoting activity	1675:1705	the adhesion-promoting activity of WTCM on Pdia3 MEFs	1675:1727	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	6	60	theme	fibronectin	1279:1289	arg1	immunodepletion					1260:1274	immunodepletion	1260:1274	immunodepletion of fibronectin	1260:1289	Rescue depended on PDIA3 activity in WT-MEFs and was not prevented by immunodepletion of fibronectin.
35196163	4	61	from	inhibition	731:740	arg1	HDF					745:747	HDF	745:747	HDF	745:747	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	2	62	theme	novel	502:506	arg1	protein					526:532	a novel candidate binding protein	500:532	a novel candidate binding protein	500:532	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	62	theme	novel	502:506	arg1	isomerase					470:478	protein disulfide isomerase A3	452:481	protein disulfide isomerase A3 (PDIA3/ERp57)	452:495	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	8	63	theme	WT-MEFs	1475:1481	arg1	secretomes					1461:1470	the heparin-binding secretomes	1441:1470	the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs	1441:1496	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	5	64	theme	cellular	1041:1048	arg1	phenotypes					1050:1059	These cellular phenotypes	1035:1059	These cellular phenotypes of Pdia3 MEFs	1035:1073	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	8	65	theme	Pdia3	1487:1491	arg1	MEFs					1493:1496	Pdia3 MEFs	1487:1496	Pdia3 MEFs	1487:1496	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	9	66	theme	WTCM	1710:1713	arg1	activity					1698:1705	the adhesion-promoting activity	1675:1705	the adhesion-promoting activity of WTCM on Pdia3 MEFs	1675:1727	Of these, cell communication network 2 (CCN2) was identified to be necessary for the adhesion-promoting activity of WTCM on Pdia3 MEFs and to bind TSP1.
35196163	4	67	theme	PDIA3	689:693	arg1	function					695:702	PDIA3 function	689:702	PDIA3 function	689:702	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	0	68	theme	matrix-rich	23:33	arg1	secretome					35:43	a matrix-rich secretome	21:43	a matrix-rich secretome that stimulates fibroblast adhesion through CCN2	21:92	PDIA3/ERp57 promotes a matrix-rich secretome that stimulates fibroblast adhesion through CCN2.
35196163	1	69	theme	intracellular	265:277	arg1	associations					279:290	its intracellular associations	261:290	its intracellular associations prior to secretion	261:309	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	1	70	theme	glycoprotein	113:124	arg1	TSP1					144:147	TSP1	144:147	TSP1	144:147	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	1	70	theme	glycoprotein	113:124	arg1	thrombospondin-1					126:141	The matricellular glycoprotein thrombospondin-1	95:141	The matricellular glycoprotein thrombospondin-1 (TSP1)	95:148	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	2	71	theme	TSP1	413:416	arg1	screen					430:435	a biotin ligase-based TSP1 interactome screen	391:435	a biotin ligase-based TSP1 interactome screen	391:435	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	10	72	theme	fibroblast	1771:1780	arg1	target					1886:1891	a translational target	1870:1891	a translational target	1870:1891	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	10	72	theme	fibroblast	1771:1780	arg1	production					1782:1791	fibroblast production	1771:1791	fibroblast production of an ECM-rich, proadhesive microenvironment	1771:1836	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	2	73	theme	biotin	393:398	arg1	screen					430:435	a biotin ligase-based TSP1 interactome screen	391:435	a biotin ligase-based TSP1 interactome screen	391:435	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	1	74	theme	prior	292:296	arg1	associations					279:290	its intracellular associations	261:290	its intracellular associations prior to secretion	261:309	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	2	75	theme	disulfide	460:468	arg1	PDIA3/ERp57					484:494	PDIA3/ERp57	484:494	PDIA3/ERp57	484:494	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	75	theme	disulfide	460:468	arg1	protein					526:532	a novel candidate binding protein	500:532	a novel candidate binding protein	500:532	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	2	75	theme	disulfide	460:468	arg1	isomerase					470:478	protein disulfide isomerase A3	452:481	protein disulfide isomerase A3 (PDIA3/ERp57)	452:495	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	8	76	theme	growth	1526:1531	arg1	proteins					1540:1547	multiple ECM and growth factor proteins	1509:1547	proteins	1540:1547	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	4	77	theme	mouse	761:765	arg1	fibroblasts					774:784	Pdia3 mouse embryo fibroblasts	755:784	Pdia3 mouse embryo fibroblasts (Pdia3 MEFs)	755:797	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	4	77	theme	mouse	761:765	arg1	MEFs					793:796	Pdia3 MEFs	787:796	Pdia3 MEFs	787:796	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	8	78	from	proteomics	1405:1414	arg1	secretomes					1461:1470	the heparin-binding secretomes	1441:1470	the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs	1441:1496	Comparative quantitative tandem-mass-tag proteomics and functional assays on the heparin-binding secretomes of WT-MEFs and Pdia3 MEFs identified multiple ECM and growth factor proteins to be downregulated in the CM of Pdia3 MEFs.
35196163	0	79	theme	fibroblast	61:70	arg1	adhesion					72:79	fibroblast adhesion	61:79	fibroblast adhesion	61:79	PDIA3/ERp57 promotes a matrix-rich secretome that stimulates fibroblast adhesion through CCN2.
35196163	4	80	theme	reduced	949:955	arg1	spreading					962:970	reduced cell spreading	949:970	reduced cell spreading	949:970	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	10	81	theme	ECM-rich	1799:1806	arg1	microenvironment					1821:1836	an ECM-rich, proadhesive microenvironment	1796:1836	an ECM-rich, proadhesive microenvironment	1796:1836	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	2	82	theme	novel	326:330	arg1	interactions					346:357	novel intracellular interactions	326:357	novel intracellular interactions of TSP1	326:365	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	5	83	theme	conditioned	1106:1116	arg1	WTCM					1126:1129	WTCM	1126:1129	WTCM	1126:1129	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	5	83	theme	conditioned	1106:1116	arg1	medium					1118:1123	conditioned medium	1106:1123	conditioned medium (WTCM)	1106:1130	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
35196163	4	84	theme	reduced	1010:1016	arg1	adhesions					1024:1032	reduced focal adhesions	1010:1032	reduced focal adhesions	1010:1032	Loss of PDIA3 function, either by pharmacological inhibition in HDF or in Pdia3 mouse embryo fibroblasts (Pdia3 MEFs), led to alterations in the composition of cell-derived extracellular matrix, involving changed abundance of fibronectin and TSP1, was correlated with reduced cell spreading, altered organization of F-actin, and reduced focal adhesions.
35196163	3	85	theme	endoplasmic	624:634	arg1	reticulum					636:644	the endoplasmic reticulum	620:644	the endoplasmic reticulum of human dermal fibroblasts (HDF)	620:678	In validation, TSP1 and PDIA3 were established to bind in vitro and to colocalize in the endoplasmic reticulum of human dermal fibroblasts (HDF).
35196163	10	86	dep	ECM-rich	1799:1806	arg1	proadhesive					1809:1819	proadhesive	1809:1819	proadhesive	1809:1819	Thus, PDIA3 coordinates fibroblast production of an ECM-rich, proadhesive microenvironment, with implications for PDIA3 as a translational target.
35196163	1	87	contain	has	150:152	arg2	roles					162:166	complex roles	154:166	complex roles	154:166	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	1	87	contain	has	150:152	arg1	TSP1					144:147	TSP1	144:147	TSP1	144:147	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	1	87	contain	has	150:152	arg1	thrombospondin-1					126:141	The matricellular glycoprotein thrombospondin-1	95:141	The matricellular glycoprotein thrombospondin-1 (TSP1)	95:148	The matricellular glycoprotein thrombospondin-1 (TSP1) has complex roles in the extracellular matrix (ECM) and at cell surfaces, but relatively little is known about its intracellular associations prior to secretion.
35196163	2	88	theme	TSP1	362:365	arg1	interactions					346:357	novel intracellular interactions	326:357	novel intracellular interactions of TSP1	326:365	To search for novel intracellular interactions of TSP1 in situ, we carried out a biotin ligase-based TSP1 interactome screen and identified protein disulfide isomerase A3 (PDIA3/ERp57) as a novel candidate binding protein.
35196163	5	89	theme	wild-type	1169:1177	arg1	-MEFs					1183:1187	wild-type (WT)-MEFs	1169:1187	wild-type (WT)-MEFs	1169:1187	These cellular phenotypes of Pdia3 MEFs were normalized by exposure to conditioned medium (WTCM) or extracellular matrix (WTECM) from wild-type (WT)-MEFs.
37159233	13	0	theme	expressed	1994:2002	arg1	CA9					2036:2038	CA9	2036:2038	CA9	2036:2038	Gene expression studies indicated that the most differentially expressed genes in these co-cultures were CA9, HLA-DQA1, TMPRSS2, FPR1, OAS2, and KLRD1.
37159233	13	0	theme	expressed	1994:2002	arg1	genes					2004:2008	the most differentially expressed genes	1970:2008	the most differentially expressed genes in these co-cultures	1970:2029	Gene expression studies indicated that the most differentially expressed genes in these co-cultures were CA9, HLA-DQA1, TMPRSS2, FPR1, OAS2, and KLRD1.
37159233	1	1	theme	invasive	238:245	arg1	nature					247:252	its highly invasive nature	227:252	its highly invasive nature	227:252	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	10	2	theme	U87	1560:1562	arg1	cells					1574:1578	U87 and LN229 cells	1560:1578	U87 and LN229 cells	1560:1578	U87 and LN229 cells displayed different migration behaviors.
37159233	2	3	theme	tumour	262:267	arg1	TME					287:289	TME	287:289	TME	287:289	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	3	theme	tumour	262:267	arg1	microenvironment					269:284	The GB tumour microenvironment	255:284	The GB tumour microenvironment (TME)	255:290	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	14	4	theme	greater	2196:2202	arg1	influence					2204:2212	greater influence	2196:2212	greater influence on U87 than LN229	2196:2230	Most differentially expressed genes were related to immune response, inflammation, and cytokine signalling, with greater influence on U87 than LN229.
37159233	11	5	theme	single	1657:1662	arg1	cells					1664:1668	single cells	1657:1668	single cells	1657:1668	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	3	6	theme	culture	484:490	arg1	models					492:497	culture models	484:497	culture models	484:497	As such, researchers have attempted to create biomaterials and culture models that mimic features of TME complexity.
37159233	5	7	theme	cell	818:821	arg1	type					823:826	the normal cell type	807:826	the normal cell type from which GB likely derives	807:855	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	5	7	theme	cell	818:821	arg1	interactions					761:772	interactions	761:772	interactions between GB cells and astrocytes	761:804	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	6	8	theme	fixed	1125:1129	arg1	astrocytes					1131:1140	dispersed live or fixed astrocytes	1107:1140	dispersed live or fixed astrocytes	1107:1140	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	3	9	theme	TME	522:524	arg1	complexity					526:535	TME complexity	522:535	TME complexity	522:535	As such, researchers have attempted to create biomaterials and culture models that mimic features of TME complexity.
37159233	8	10	from	distance	1423:1430	arg1	hydrogels					1441:1449	these hydrogels	1435:1449	these hydrogels	1435:1449	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	6	11	theme	GB	932:933	arg1	multi-spheres					935:947	GB multi-spheres	932:947	GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture)	932:1001	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	12	theme	live	1117:1120	arg1	astrocytes					1131:1140	dispersed live or fixed astrocytes	1107:1140	dispersed live or fixed astrocytes	1107:1140	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	10	13	theme	LN229	1568:1572	arg1	cells					1574:1578	U87 and LN229 cells	1560:1578	U87 and LN229 cells	1560:1578	U87 and LN229 cells displayed different migration behaviors.
37159233	1	14	theme	survival	194:201	arg1	rate					203:206	a low survival rate	188:206	a low survival rate	188:206	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	4	15	theme	3D	608:609	arg1	culture					616:622	3D cell culture	608:622	3D cell culture	608:622	Hydrogel materials have been particularly popular because they enable 3D cell culture and mimic TME mechanical properites and chemical composition.
37159233	5	16	theme	GB	782:783	arg1	cells					785:789	GB cells	782:789	GB cells	782:789	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	6	17	theme	culture	898:904	arg1	mono-spheres					1080:1091	GB-only mono-spheres	1072:1091	GB-only mono-spheres cultured with dispersed live or fixed astrocytes	1072:1140	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	17	theme	culture	898:904	arg1	configurations					906:919	three different spheroid culture configurations	873:919	three different spheroid culture configurations	873:919	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	17	theme	culture	898:904	arg1	multi-spheres					935:947	GB multi-spheres	932:947	GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture)	932:1001	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	17	theme	culture	898:904	arg1	mono-spheres					1012:1023	GB-only mono-spheres	1004:1023	GB-only mono-spheres cultured with astrocyte-conditioned media	1004:1065	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	10	18	theme	migration	1600:1608	arg1	behaviors					1610:1618	different migration behaviors	1590:1618	different migration behaviors	1590:1618	U87 and LN229 cells displayed different migration behaviors.
37159233	6	19	theme	different	879:887	arg1	mono-spheres					1080:1091	GB-only mono-spheres	1072:1091	GB-only mono-spheres cultured with dispersed live or fixed astrocytes	1072:1140	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	19	theme	different	879:887	arg1	configurations					906:919	three different spheroid culture configurations	873:919	three different spheroid culture configurations	873:919	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	19	theme	different	879:887	arg1	multi-spheres					935:947	GB multi-spheres	932:947	GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture)	932:1001	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	19	theme	different	879:887	arg1	mono-spheres					1012:1023	GB-only mono-spheres	1004:1023	GB-only mono-spheres cultured with astrocyte-conditioned media	1004:1065	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	4	20	theme	TME	634:636	arg1	properites					649:658	TME mechanical properites	634:658	TME mechanical properites	634:658	Hydrogel materials have been particularly popular because they enable 3D cell culture and mimic TME mechanical properites and chemical composition.
37159233	13	21	theme	expression	1936:1945	arg1	studies					1947:1953	Gene expression studies	1931:1953	Gene expression studies	1931:1953	Gene expression studies indicated that the most differentially expressed genes in these co-cultures were CA9, HLA-DQA1, TMPRSS2, FPR1, OAS2, and KLRD1.
37159233	11	22	theme	mono-sphere	1745:1755	arg1	cultures					1782:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	15	23	theme	specific	2325:2332	arg1	differences					2334:2344	cell line specific differences	2315:2344	cell line specific differences in migration	2315:2357	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	8	24	theme	fluorescence	1284:1295	arg1	microscopy					1297:1306	time-lapse fluorescence microscopy	1273:1306	time-lapse fluorescence microscopy	1273:1306	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	11	25	theme	multi-sphere	1728:1739	arg1	cultures					1782:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	7	26	theme	human	1189:1193	arg1	astrocytes					1195:1204	primary human astrocytes	1181:1204	primary human astrocytes	1181:1204	Using U87 and LN229 GB cell lines and primary human astrocytes, we investigated material and experiment variability.
37159233	9	27	theme	gene	1501:1504	arg1	analysis					1517:1524	gene expression analysis	1501:1524	gene expression analysis from cells cultured in hydrogels	1501:1557	Finally, we developed methods to extract RNA for gene expression analysis from cells cultured in hydrogels.
37159233	5	28	theme	acid	727:730	arg1	material					741:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	5	29	used	used	695:698	arg2	we					692:693	we	692:693	we	692:693	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	1	30	theme	astrocytic	159:168	arg1	tumour					176:181	an astrocytic brain tumour	156:181	an astrocytic brain tumour with a low survival rate	156:206	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	1	30	theme	astrocytic	159:168	arg1	Glioblastoma					135:146	Glioblastoma	135:146	Glioblastoma (GB)	135:151	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	8	31	theme	invasive	1319:1326	arg1	potential					1328:1336	invasive potential	1319:1336	invasive potential	1319:1336	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	11	32	theme	dispersed	1762:1770	arg1	cultures					1782:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	0	33	theme	3D	96:97	arg1	hydrogels					124:132	3D collagen-hyaluronic acid hydrogels	96:132	3D collagen-hyaluronic acid hydrogels	96:132	Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.
37159233	2	34	theme	cell	352:355	arg1	types					357:361	brain cell types	346:361	brain cell types	346:361	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	0	35	theme	acid	119:122	arg1	hydrogels					124:132	3D collagen-hyaluronic acid hydrogels	96:132	3D collagen-hyaluronic acid hydrogels	96:132	Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.
37159233	15	36	theme	GB-astrocyte	2385:2396	arg1	crosstalk					2398:2406	differential GB-astrocyte crosstalk	2372:2406	differential GB-astrocyte crosstalk	2372:2406	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	12	37	theme	dispersed	1901:1909	arg1	cultures					1921:1928	monosphere plus dispersed astrocyte cultures	1885:1928	monosphere plus dispersed astrocyte cultures	1885:1928	In contrast, LN229 migration exhibited features of collective migration and was increased in monosphere plus dispersed astrocyte cultures.
37159233	2	38	theme	local	398:402	arg1	cues					415:418	local mechanical cues	398:418	local mechanical cues	398:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	6	39	dep	cells	973:977	arg1	i.e.					950:953	i.e.	950:953	i.e.	950:953	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	14	40	theme	expressed	2103:2111	arg1	genes					2113:2117	Most differentially expressed genes	2083:2117	Most differentially expressed genes	2083:2117	Most differentially expressed genes were related to immune response, inflammation, and cytokine signalling, with greater influence on U87 than LN229.
37159233	5	41	theme	3D	702:703	arg1	material					741:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	7	42	theme	U87	1149:1151	arg1	lines					1171:1175	U87 and LN229 GB cell lines	1149:1175	U87 and LN229 GB cell lines	1149:1175	Using U87 and LN229 GB cell lines and primary human astrocytes, we investigated material and experiment variability.
37159233	15	43	theme	hydrogel	2266:2273	arg1	models					2286:2291	3D in vitro hydrogel co-culture models	2254:2291	3D in vitro hydrogel co-culture models	2254:2291	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	6	44	theme	GB-only	1004:1010	arg1	mono-spheres					1012:1023	GB-only mono-spheres	1004:1023	GB-only mono-spheres cultured with astrocyte-conditioned media	1004:1065	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	2	45	theme	anatomical	371:380	arg1	structures					382:391	unique anatomical structures	364:391	unique anatomical structures	364:391	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	0	46	theme	tumour	24:29	arg1	growth					38:43	glioblastoma tumour sphere growth	11:43	glioblastoma tumour sphere growth	11:43	Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.
37159233	15	47	theme	in	2257:2258	arg1	models					2286:2291	3D in vitro hydrogel co-culture models	2254:2291	3D in vitro hydrogel co-culture models	2254:2291	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	4	48	theme	mechanical	638:647	arg1	properites					649:658	TME mechanical properites	634:658	TME mechanical properites	634:658	Hydrogel materials have been particularly popular because they enable 3D cell culture and mimic TME mechanical properites and chemical composition.
37159233	8	49	theme	migration	1373:1381	arg1	capacity					1383:1390	migration capacity	1373:1390	migration capacity	1373:1390	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	5	50	theme	normal	811:816	arg1	type					823:826	the normal cell type	807:826	the normal cell type from which GB likely derives	807:855	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	5	50	theme	normal	811:816	arg1	interactions					761:772	interactions	761:772	interactions between GB cells and astrocytes	761:804	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	12	51	theme	monosphere	1885:1894	arg1	cultures					1921:1928	monosphere plus dispersed astrocyte cultures	1885:1928	monosphere plus dispersed astrocyte cultures	1885:1928	In contrast, LN229 migration exhibited features of collective migration and was increased in monosphere plus dispersed astrocyte cultures.
37159233	6	52	theme	spheroid	982:989	arg1	co-culture					991:1000	spheroid co-culture	982:1000	spheroid co-culture	982:1000	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	2	53	theme	cues	415:418	arg1	matrix					319:324	its extracellular matrix	301:324	its extracellular matrix (ECM)	301:330	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	53	theme	cues	415:418	arg1	cues					415:418	local mechanical cues	398:418	local mechanical cues	398:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	53	theme	cues	415:418	arg1	variety					335:341	a variety	333:341	a variety of brain cell types, unique anatomical structures, and local mechanical cues	333:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	53	theme	cues	415:418	arg1	structures					382:391	unique anatomical structures	364:391	unique anatomical structures	364:391	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	53	theme	cues	415:418	arg1	types					357:361	brain cell types	346:361	brain cell types	346:361	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	5	54	theme	collagen	705:712	arg1	material					741:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	14	55	with	related	2124:2130	arg1	influence					2204:2212	greater influence	2196:2212	greater influence on U87 than LN229	2196:2230	Most differentially expressed genes were related to immune response, inflammation, and cytokine signalling, with greater influence on U87 than LN229.
37159233	12	56	theme	collective	1843:1852	arg1	migration					1854:1862	collective migration	1843:1862	collective migration	1843:1862	In contrast, LN229 migration exhibited features of collective migration and was increased in monosphere plus dispersed astrocyte cultures.
37159233	15	57	used	used	2300:2303	arg2	models					2286:2291	3D in vitro hydrogel co-culture models	2254:2291	3D in vitro hydrogel co-culture models	2254:2291	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	8	58	theme	weight-averaged	1397:1411	arg1	distance					1423:1430	weight-averaged migration distance	1397:1430	weight-averaged migration distance	1397:1430	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	2	59	theme	extracellular	305:317	arg1	structures					382:391	unique anatomical structures	364:391	unique anatomical structures	364:391	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	59	theme	extracellular	305:317	arg1	ECM					327:329	ECM	327:329	ECM	327:329	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	59	theme	extracellular	305:317	arg1	types					357:361	brain cell types	346:361	brain cell types	346:361	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	59	theme	extracellular	305:317	arg1	matrix					319:324	its extracellular matrix	301:324	its extracellular matrix (ECM)	301:330	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	59	theme	extracellular	305:317	arg1	cues					415:418	local mechanical cues	398:418	local mechanical cues	398:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	59	theme	extracellular	305:317	arg1	variety					335:341	a variety	333:341	a variety of brain cell types, unique anatomical structures, and local mechanical cues	333:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	15	60	theme	cell	2315:2318	arg1	differences					2334:2344	cell line specific differences	2315:2344	cell line specific differences in migration	2315:2357	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	1	61	with	tumour	176:181	arg1	rate					203:206	a low survival rate	188:206	a low survival rate	188:206	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	6	62	theme	GB-only	1072:1078	arg1	mono-spheres					1080:1091	GB-only mono-spheres	1072:1091	GB-only mono-spheres cultured with dispersed live or fixed astrocytes	1072:1140	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	11	63	theme	astrocytes	1709:1718	arg1	numbers					1698:1704	higher numbers	1691:1704	higher numbers of astrocytes	1691:1718	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	11	64	theme	U87	1621:1623	arg1	migration					1625:1633	U87 migration	1621:1633	U87 migration	1621:1633	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	15	65	dep	in	2257:2258	arg1	vitro					2260:2264	vitro	2260:2264	vitro	2260:2264	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	12	66	theme	LN229	1805:1809	arg1	migration					1811:1819	LN229 migration	1805:1819	LN229 migration	1805:1819	In contrast, LN229 migration exhibited features of collective migration and was increased in monosphere plus dispersed astrocyte cultures.
37159233	6	67	theme	astrocyte-conditioned	1039:1059	arg1	media					1061:1065	astrocyte-conditioned media	1039:1065	astrocyte-conditioned media	1039:1065	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	13	68	from	genes	2004:2008	arg1	co-cultures					2019:2029	these co-cultures	2013:2029	these co-cultures	2013:2029	Gene expression studies indicated that the most differentially expressed genes in these co-cultures were CA9, HLA-DQA1, TMPRSS2, FPR1, OAS2, and KLRD1.
37159233	4	69	theme	Hydrogel	538:545	arg1	materials					547:555	Hydrogel materials	538:555	Hydrogel materials	538:555	Hydrogel materials have been particularly popular because they enable 3D cell culture and mimic TME mechanical properites and chemical composition.
37159233	0	70	with	interaction	62:72	arg1	astrocytes					79:88	astrocytes	79:88	astrocytes using 3D collagen-hyaluronic acid hydrogels	79:132	Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.
37159233	2	71	theme	GB	259:260	arg1	TME					287:289	TME	287:289	TME	287:289	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	71	theme	GB	259:260	arg1	microenvironment					269:284	The GB tumour microenvironment	255:284	The GB tumour microenvironment (TME)	255:290	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	8	72	from	capacity	1383:1390	arg1	hydrogels					1441:1449	these hydrogels	1435:1449	these hydrogels	1435:1449	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	1	73	theme	brain	170:174	arg1	tumour					176:181	an astrocytic brain tumour	156:181	an astrocytic brain tumour with a low survival rate	156:206	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	1	73	theme	brain	170:174	arg1	Glioblastoma					135:146	Glioblastoma	135:146	Glioblastoma (GB)	135:151	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	14	74	from	influence	2204:2212	arg1	U87					2217:2219	U87	2217:2219	U87	2217:2219	Most differentially expressed genes were related to immune response, inflammation, and cytokine signalling, with greater influence on U87 than LN229.
37159233	4	75	theme	cell	611:614	arg1	culture					616:622	3D cell culture	608:622	3D cell culture	608:622	Hydrogel materials have been particularly popular because they enable 3D cell culture and mimic TME mechanical properites and chemical composition.
37159233	10	76	theme	different	1590:1598	arg1	behaviors					1610:1618	different migration behaviors	1590:1618	different migration behaviors	1590:1618	U87 and LN229 cells displayed different migration behaviors.
37159233	1	77	theme	low	190:192	arg1	rate					203:206	a low survival rate	188:206	a low survival rate	188:206	Glioblastoma (GB) is an astrocytic brain tumour with a low survival rate, partly because of its highly invasive nature.
37159233	6	78	theme	dispersed	1107:1115	arg1	astrocytes					1131:1140	dispersed live or fixed astrocytes	1107:1140	dispersed live or fixed astrocytes	1107:1140	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	15	79	theme	differential	2372:2383	arg1	crosstalk					2398:2406	differential GB-astrocyte crosstalk	2372:2406	differential GB-astrocyte crosstalk	2372:2406	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	7	80	theme	GB	1163:1164	arg1	lines					1171:1175	U87 and LN229 GB cell lines	1149:1175	U87 and LN229 GB cell lines	1149:1175	Using U87 and LN229 GB cell lines and primary human astrocytes, we investigated material and experiment variability.
37159233	3	81	theme	complexity	526:535	arg1	features					510:517	features	510:517	features of TME complexity	510:535	As such, researchers have attempted to create biomaterials and culture models that mimic features of TME complexity.
37159233	6	82	theme	spheroid	889:896	arg1	mono-spheres					1080:1091	GB-only mono-spheres	1072:1091	GB-only mono-spheres cultured with dispersed live or fixed astrocytes	1072:1140	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	82	theme	spheroid	889:896	arg1	configurations					906:919	three different spheroid culture configurations	873:919	three different spheroid culture configurations	873:919	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	82	theme	spheroid	889:896	arg1	multi-spheres					935:947	GB multi-spheres	932:947	GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture)	932:1001	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	6	82	theme	spheroid	889:896	arg1	mono-spheres					1012:1023	GB-only mono-spheres	1004:1023	GB-only mono-spheres cultured with astrocyte-conditioned media	1004:1065	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	4	83	theme	chemical	664:671	arg1	composition					673:683	chemical composition	664:683	chemical composition	664:683	Hydrogel materials have been particularly popular because they enable 3D cell culture and mimic TME mechanical properites and chemical composition.
37159233	13	84	theme	Gene	1931:1934	arg1	studies					1947:1953	Gene expression studies	1931:1953	Gene expression studies	1931:1953	Gene expression studies indicated that the most differentially expressed genes in these co-cultures were CA9, HLA-DQA1, TMPRSS2, FPR1, OAS2, and KLRD1.
37159233	6	85	dep	multi-spheres	935:947	arg1	cells					973:977	GB and astrocyte cells	956:977	GB and astrocyte cells in spheroid co-culture	956:1000	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	11	86	theme	plus	1757:1760	arg1	cultures					1782:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	7	87	theme	primary	1181:1187	arg1	astrocytes					1195:1204	primary human astrocytes	1181:1204	primary human astrocytes	1181:1204	Using U87 and LN229 GB cell lines and primary human astrocytes, we investigated material and experiment variability.
37159233	6	88	theme	astrocyte	963:971	arg1	cells					973:977	GB and astrocyte cells	956:977	GB and astrocyte cells in spheroid co-culture	956:1000	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	15	89	from	differences	2334:2344	arg1	migration					2349:2357	migration	2349:2357	migration	2349:2357	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	14	90	theme	cytokine	2170:2177	arg1	signalling					2179:2188	cytokine signalling	2170:2188	cytokine signalling	2170:2188	Most differentially expressed genes were related to immune response, inflammation, and cytokine signalling, with greater influence on U87 than LN229.
37159233	8	91	theme	time-lapse	1273:1282	arg1	microscopy					1297:1306	time-lapse fluorescence microscopy	1273:1306	time-lapse fluorescence microscopy	1273:1306	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	7	92	theme	cell	1166:1169	arg1	lines					1171:1175	U87 and LN229 GB cell lines	1149:1175	U87 and LN229 GB cell lines	1149:1175	Using U87 and LN229 GB cell lines and primary human astrocytes, we investigated material and experiment variability.
37159233	5	93	theme	I-hyaluronic	714:725	arg1	material					741:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	9	94	theme	expression	1506:1515	arg1	analysis					1517:1524	gene expression analysis	1501:1524	gene expression analysis from cells cultured in hydrogels	1501:1557	Finally, we developed methods to extract RNA for gene expression analysis from cells cultured in hydrogels.
37159233	6	95	theme	GB	956:957	arg1	cells					973:977	GB and astrocyte cells	956:977	GB and astrocyte cells in spheroid co-culture	956:1000	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	14	96	theme	immune	2135:2140	arg1	response					2142:2149	immune response	2135:2149	immune response	2135:2149	Most differentially expressed genes were related to immune response, inflammation, and cytokine signalling, with greater influence on U87 than LN229.
37159233	0	97	theme	collagen-hyaluronic	99:117	arg1	hydrogels					124:132	3D collagen-hyaluronic acid hydrogels	96:132	3D collagen-hyaluronic acid hydrogels	96:132	Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.
37159233	7	98	dep	material	1223:1230	arg1	variability					1247:1257	variability	1247:1257	variability	1247:1257	Using U87 and LN229 GB cell lines and primary human astrocytes, we investigated material and experiment variability.
37159233	9	99	from	cells	1531:1535	arg1	analysis					1517:1524	gene expression analysis	1501:1524	gene expression analysis from cells cultured in hydrogels	1501:1557	Finally, we developed methods to extract RNA for gene expression analysis from cells cultured in hydrogels.
37159233	7	100	theme	LN229	1157:1161	arg1	lines					1171:1175	U87 and LN229 GB cell lines	1149:1175	U87 and LN229 GB cell lines	1149:1175	Using U87 and LN229 GB cell lines and primary human astrocytes, we investigated material and experiment variability.
37159233	6	101	from	cells	973:977	arg1	co-culture					991:1000	spheroid co-culture	982:1000	spheroid co-culture	982:1000	We demonstrate three different spheroid culture configurations, including GB multi-spheres (i.e., GB and astrocyte cells in spheroid co-culture), GB-only mono-spheres cultured with astrocyte-conditioned media, and GB-only mono-spheres cultured with dispersed live or fixed astrocytes.
37159233	11	102	theme	astrocyte	1772:1780	arg1	cultures					1782:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	both multi-sphere and mono-sphere plus dispersed astrocyte cultures	1723:1789	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	12	103	theme	astrocyte	1911:1919	arg1	cultures					1921:1928	monosphere plus dispersed astrocyte cultures	1885:1928	monosphere plus dispersed astrocyte cultures	1885:1928	In contrast, LN229 migration exhibited features of collective migration and was increased in monosphere plus dispersed astrocyte cultures.
37159233	2	104	theme	brain	346:350	arg1	types					357:361	brain cell types	346:361	brain cell types	346:361	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	0	105	theme	glioblastoma	11:22	arg1	growth					38:43	glioblastoma tumour sphere growth	11:43	glioblastoma tumour sphere growth	11:43	Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.
37159233	0	106	theme	sphere	31:36	arg1	growth					38:43	glioblastoma tumour sphere growth	11:43	glioblastoma tumour sphere growth	11:43	Evaluating glioblastoma tumour sphere growth and migration in interaction with astrocytes using 3D collagen-hyaluronic acid hydrogels.
37159233	15	107	theme	co-culture	2275:2284	arg1	models					2286:2291	3D in vitro hydrogel co-culture models	2254:2291	3D in vitro hydrogel co-culture models	2254:2291	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	2	108	theme	structures	382:391	arg1	matrix					319:324	its extracellular matrix	301:324	its extracellular matrix (ECM)	301:330	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	108	theme	structures	382:391	arg1	cues					415:418	local mechanical cues	398:418	local mechanical cues	398:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	108	theme	structures	382:391	arg1	variety					335:341	a variety	333:341	a variety of brain cell types, unique anatomical structures, and local mechanical cues	333:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	108	theme	structures	382:391	arg1	structures					382:391	unique anatomical structures	364:391	unique anatomical structures	364:391	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	108	theme	structures	382:391	arg1	types					357:361	brain cell types	346:361	brain cell types	346:361	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	11	109	theme	higher	1691:1696	arg1	numbers					1698:1704	higher numbers	1691:1704	higher numbers of astrocytes	1691:1718	U87 migration occurred primarily as single cells and was reduced with higher numbers of astrocytes in both multi-sphere and mono-sphere plus dispersed astrocyte cultures.
37159233	8	110	theme	sphere	1360:1365	arg1	size					1367:1370	sphere size	1360:1370	sphere size	1360:1370	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	2	111	theme	unique	364:369	arg1	structures					382:391	unique anatomical structures	364:391	unique anatomical structures	364:391	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	5	112	theme	hydrogel	732:739	arg1	material					741:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	a 3D collagen I-hyaluronic acid hydrogel material	700:748	Here, we used a 3D collagen I-hyaluronic acid hydrogel material to explore interactions between GB cells and astrocytes, the normal cell type from which GB likely derives.
37159233	2	113	theme	types	357:361	arg1	matrix					319:324	its extracellular matrix	301:324	its extracellular matrix (ECM)	301:330	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	113	theme	types	357:361	arg1	cues					415:418	local mechanical cues	398:418	local mechanical cues	398:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	113	theme	types	357:361	arg1	variety					335:341	a variety	333:341	a variety of brain cell types, unique anatomical structures, and local mechanical cues	333:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	113	theme	types	357:361	arg1	structures					382:391	unique anatomical structures	364:391	unique anatomical structures	364:391	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	2	113	theme	types	357:361	arg1	types					357:361	brain cell types	346:361	brain cell types	346:361	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	15	114	theme	3D	2254:2255	arg1	models					2286:2291	3D in vitro hydrogel co-culture models	2254:2291	3D in vitro hydrogel co-culture models	2254:2291	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	12	115	theme	migration	1854:1862	arg1	features					1831:1838	features	1831:1838	features of collective migration	1831:1862	In contrast, LN229 migration exhibited features of collective migration and was increased in monosphere plus dispersed astrocyte cultures.
37159233	2	116	theme	mechanical	404:413	arg1	cues					415:418	local mechanical cues	398:418	local mechanical cues	398:418	The GB tumour microenvironment (TME) includes its extracellular matrix (ECM), a variety of brain cell types, unique anatomical structures, and local mechanical cues.
37159233	8	117	used	used	1268:1271	arg2	We					1260:1261	We	1260:1261	We	1260:1261	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	15	118	theme	line	2320:2323	arg1	differences					2334:2344	cell line specific differences	2315:2344	cell line specific differences in migration	2315:2357	These data show that 3D in vitro hydrogel co-culture models can be used to reveal cell line specific differences in migration and to study differential GB-astrocyte crosstalk.
37159233	8	119	theme	migration	1413:1421	arg1	distance					1423:1430	weight-averaged migration distance	1397:1430	weight-averaged migration distance	1397:1430	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
37159233	8	120	from	size	1367:1370	arg1	hydrogels					1441:1449	these hydrogels	1435:1449	these hydrogels	1435:1449	We then used time-lapse fluorescence microscopy to measure invasive potential by characterizing the sphere size, migration capacity, and weight-averaged migration distance in these hydrogels.
36376753	0	0	theme	regulatory	67:76	arg1	role					78:81	a key regulatory role	61:81	a key regulatory role for the class II T6P synthase-like proteins	61:125	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	2	1	theme	metabolic	483:491	arg1	enzymes					493:499	metabolic enzymes	483:499	metabolic enzymes	483:499	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	6	2	theme	metabolic	1329:1337	arg1	stress					1339:1344	metabolic stress	1329:1344	metabolic stress	1329:1344	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	1	3	theme	central	132:138	arg1	SnRK1					160:164	The central metabolic regulator SnRK1	128:164	The central metabolic regulator SnRK1	128:164	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	0	4	theme	key	63:65	arg1	role					78:81	a key regulatory role	61:81	a key regulatory role for the class II T6P synthase-like proteins	61:125	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	2	5	used	used	332:335	arg2	we					329:330	we	329:330	we	329:330	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	1	6	theme	metabolic	140:148	arg1	SnRK1					160:164	The central metabolic regulator SnRK1	128:164	The central metabolic regulator SnRK1	128:164	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	6	7	with	interactions	1113:1124	arg1	proteins					1144:1151	the TPS-like proteins	1131:1151	the TPS-like proteins	1131:1151	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	3	8	theme	protein	709:715	arg1	landscape					729:737	the protein interaction landscape	705:737	the protein interaction landscape	705:737	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	3	9	from	insight	803:809	arg1	regulation					833:842	its plant-specific regulation	814:842	its plant-specific regulation	814:842	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	1	10	theme	detailed	242:249	arg1	insight					261:267	detailed molecular insight	242:267	detailed molecular insight into its regulation and downstream targets	242:310	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	6	11	theme	optimal	1308:1314	arg1	responses					1316:1324	optimal responses	1308:1324	optimal responses to metabolic stress	1308:1344	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	1	12	theme	molecular	251:259	arg1	insight					261:267	detailed molecular insight	242:267	detailed molecular insight into its regulation and downstream targets	242:310	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	0	13	theme	class	91:95	arg1	proteins					118:125	the class II T6P synthase-like proteins	87:125	the class II T6P synthase-like proteins	87:125	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	6	14	theme	intricate	1162:1170	arg1	connections					1172:1182	similar intricate connections	1154:1182	similar intricate connections	1154:1182	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	4	15	theme	multi-dimensional	873:889	arg1	interactome					891:901	this multi-dimensional interactome	868:901	this multi-dimensional interactome	868:901	At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins.
36376753	6	16	theme	stable	1106:1111	arg1	interactions					1113:1124	stable interactions	1106:1124	stable interactions with the TPS-like proteins	1106:1151	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	5	17	theme	Biochemical	996:1006	arg1	assays					1021:1026	Biochemical and cellular assays	996:1026	Biochemical and cellular assays	996:1026	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	1	18	theme	regulator	150:158	arg1	SnRK1					160:164	The central metabolic regulator SnRK1	128:164	The central metabolic regulator SnRK1	128:164	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	2	19	theme	transcriptional	505:519	arg1	regulators					521:530	transcriptional regulators	505:530	transcriptional regulators	505:530	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	0	20	theme	kinase	27:32	arg1	network					45:51	the plant SnRK1 kinase signalling network	11:51	the plant SnRK1 kinase signalling network	11:51	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	6	21	theme	known	1200:1204	arg1	regulators					1206:1215	known regulators	1200:1215	known regulators	1200:1215	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	4	22	theme	-like	980:984	arg1	proteins					986:993	class II T6P synthase (TPS)-like proteins	953:993	class II T6P synthase (TPS)-like proteins	953:993	At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins.
36376753	6	23	theme	SnRK1	1289:1293	arg1	activity					1295:1302	fine-tune SnRK1 activity	1279:1302	fine-tune SnRK1 activity	1279:1302	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	2	24	theme	intracellular	573:585	arg1	trafficking					587:597	intracellular trafficking	573:597	intracellular trafficking	573:597	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	2	25	theme	sucrose-dependent	368:384	arg1	processes					386:394	the sucrose-dependent processes	364:394	the sucrose-dependent processes targeted upon starvation by kinases as SnRK1	364:439	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	3	26	theme	SnRK1	773:777	arg1	heterotrimer					779:790	the SnRK1 heterotrimer	769:790	the SnRK1 heterotrimer	769:790	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	4	27	theme	interactome	891:901	arg1	intersection					852:863	the intersection	848:863	the intersection of this multi-dimensional interactome	848:901	At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins.
36376753	1	28	theme	plant	175:179	arg1	growth					181:186	plant growth	175:186	plant growth	175:186	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	0	29	theme	plant	15:19	arg1	network					45:51	the plant SnRK1 kinase signalling network	11:51	the plant SnRK1 kinase signalling network	11:51	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	3	30	theme	heterotrimer	779:790	arg1	composition					740:750	composition	740:750	composition	740:750	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	3	30	theme	heterotrimer	779:790	arg1	landscape					729:737	the protein interaction landscape	705:737	the protein interaction landscape	705:737	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	3	30	theme	heterotrimer	779:790	arg1	structure					756:764	structure	756:764	structure	756:764	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	0	31	theme	T6P	100:102	arg1	proteins					118:125	the class II T6P synthase-like proteins	87:125	the class II T6P synthase-like proteins	87:125	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	2	32	theme	SnRK1	472:476	arg1	relation					460:467	the relation	456:467	the relation of SnRK1 with metabolic enzymes and transcriptional regulators	456:530	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	6	33	theme	similar	1154:1160	arg1	connections					1172:1182	similar intricate connections	1154:1182	similar intricate connections	1154:1182	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	3	34	theme	crosslinking	667:678	arg1	spectrometry					685:696	crosslinking mass spectrometry	667:696	crosslinking mass spectrometry	667:696	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	5	35	theme	TPS-like	1038:1045	arg1	proteins					1047:1054	TPS-like proteins	1038:1054	TPS-like proteins	1038:1054	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	5	35	theme	TPS-like	1038:1045	arg1	regulators					1077:1086	negative regulators	1068:1086	negative regulators of SnRK1	1068:1095	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	4	36	with	association	927:937	arg1	proteins					986:993	class II T6P synthase (TPS)-like proteins	953:993	class II T6P synthase (TPS)-like proteins	953:993	At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins.
36376753	0	37	theme	SnRK1	21:25	arg1	network					45:51	the plant SnRK1 kinase signalling network	11:51	the plant SnRK1 kinase signalling network	11:51	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	0	38	theme	synthase-like	104:116	arg1	proteins					118:125	the class II T6P synthase-like proteins	87:125	the class II T6P synthase-like proteins	87:125	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	3	39	theme	proximity	643:651	arg1	labelling					653:661	proximity labelling	643:661	proximity labelling	643:661	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	0	40	theme	network	45:51	arg1	Mapping					0:6	Mapping	0:6	Mapping of the plant SnRK1 kinase signalling network	0:51	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	1	41	theme	downstream	293:302	arg1	targets					304:310	downstream targets	293:310	downstream targets	293:310	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	2	42	theme	trafficking	587:597	arg1	control					562:568	SnRK1 control	556:568	SnRK1 control of intracellular trafficking	556:597	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	6	43	theme	kinase	1261:1266	arg1	complex					1268:1274	an extended kinase complex	1249:1274	an extended kinase complex	1249:1274	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	6	44	theme	fine-tune	1279:1287	arg1	activity					1295:1302	fine-tune SnRK1 activity	1279:1302	fine-tune SnRK1 activity	1279:1302	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	0	45	theme	signalling	34:43	arg1	network					45:51	the plant SnRK1 kinase signalling network	11:51	the plant SnRK1 kinase signalling network	11:51	Mapping of the plant SnRK1 kinase signalling network reveals a key regulatory role for the class II T6P synthase-like proteins.
36376753	2	46	theme	SnRK1	556:560	arg1	control					562:568	SnRK1 control	556:568	SnRK1 control of intracellular trafficking	556:597	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	5	47	theme	negative	1068:1075	arg1	proteins					1047:1054	TPS-like proteins	1038:1054	TPS-like proteins	1038:1054	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	5	47	theme	negative	1068:1075	arg1	regulators					1077:1086	negative regulators	1068:1086	negative regulators of SnRK1	1068:1095	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	6	48	theme	extended	1252:1259	arg1	complex					1268:1274	an extended kinase complex	1249:1274	an extended kinase complex	1249:1274	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	4	49	theme	strong	920:925	arg1	association					927:937	a strong association	918:937	a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins	918:993	At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins.
36376753	5	50	theme	cellular	1012:1019	arg1	assays					1021:1026	Biochemical and cellular assays	996:1026	Biochemical and cellular assays	996:1026	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	4	51	theme	SnRK1	942:946	arg1	association					927:937	a strong association	918:937	a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins	918:993	At the intersection of this multi-dimensional interactome, we discovered a strong association of SnRK1 with class II T6P synthase (TPS)-like proteins.
36376753	3	52	theme	mass	680:683	arg1	spectrometry					685:696	crosslinking mass spectrometry	667:696	crosslinking mass spectrometry	667:696	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	5	53	theme	SnRK1	1091:1095	arg1	proteins					1047:1054	TPS-like proteins	1038:1054	TPS-like proteins	1038:1054	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	5	53	theme	SnRK1	1091:1095	arg1	regulators					1077:1086	negative regulators	1068:1086	negative regulators of SnRK1	1068:1095	Biochemical and cellular assays show that TPS-like proteins function as negative regulators of SnRK1.
36376753	6	54	theme	TPS-like	1135:1142	arg1	proteins					1144:1151	the TPS-like proteins	1131:1151	the TPS-like proteins	1131:1151	Next to stable interactions with the TPS-like proteins, similar intricate connections were found with known regulators, suggesting that plants utilize an extended kinase complex to fine-tune SnRK1 activity for optimal responses to metabolic stress.
36376753	1	55	theme	energy	220:225	arg1	depletion					227:235	energy depletion	220:235	energy depletion	220:235	The central metabolic regulator SnRK1 controls plant growth and survival upon activation by energy depletion, but detailed molecular insight into its regulation and downstream targets is limited.
36376753	3	56	theme	interaction	717:727	arg1	landscape					729:737	the protein interaction landscape	705:737	the protein interaction landscape	705:737	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	3	57	theme	affinity	620:627	arg1	purification					629:640	affinity purification	620:640	affinity purification	620:640	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36376753	2	58	with	SnRK1	472:476	arg1	enzymes					493:499	metabolic enzymes	483:499	metabolic enzymes	483:499	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	2	58	with	SnRK1	472:476	arg1	regulators					521:530	transcriptional regulators	505:530	transcriptional regulators	505:530	Here we used phosphoproteomics to infer the sucrose-dependent processes targeted upon starvation by kinases as SnRK1, corroborating the relation of SnRK1 with metabolic enzymes and transcriptional regulators, while also pointing to SnRK1 control of intracellular trafficking.
36376753	3	59	theme	plant-specific	818:831	arg1	regulation					833:842	its plant-specific regulation	814:842	its plant-specific regulation	814:842	Next, we integrated affinity purification, proximity labelling and crosslinking mass spectrometry to map the protein interaction landscape, composition and structure of the SnRK1 heterotrimer, providing insight in its plant-specific regulation.
36098266	6	0	theme	eco-friendly	1217:1228	arg1	sugar					1245:1249	inexpensive and eco-friendly α-cyclodextrin sugar	1201:1249	inexpensive and eco-friendly α-cyclodextrin sugar	1201:1249	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	3	1	theme	sensing	682:688	arg1	ability					690:696	The sensing ability	678:696	The sensing ability of the AuNPs with biopolymers	678:726	The sensing ability of the AuNPs with biopolymers was investigated via various analytical techniques.
36098266	6	2	theme	α-cyclodextrin	1230:1243	arg1	sugar					1245:1249	inexpensive and eco-friendly α-cyclodextrin sugar	1201:1249	inexpensive and eco-friendly α-cyclodextrin sugar	1201:1249	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	8	3	theme	proposed	1448:1455	arg1	process					1472:1478	the proposed gold-recycling process	1444:1478	the proposed gold-recycling process	1444:1478	In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth.
36098266	2	4	theme	separation	569:578	arg1	studies/recovery					580:595	exquisitely gold separation studies/recovery	552:595	exquisitely gold separation studies/recovery	552:595	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	6	5	theme	inexpensive	1201:1211	arg1	sugar					1245:1249	inexpensive and eco-friendly α-cyclodextrin sugar	1201:1249	inexpensive and eco-friendly α-cyclodextrin sugar	1201:1249	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	6	from	kits	1148:1151	arg1	recovery					1089:1096	the recovery	1085:1096	the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1085:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	6	from	kits	1148:1151	arg1	traces					1106:1111	gold traces	1101:1111	gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1101:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	7	from	kits	1176:1179	arg1	recovery					1089:1096	the recovery	1085:1096	the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1085:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	7	from	kits	1176:1179	arg1	traces					1106:1111	gold traces	1101:1111	gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1101:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	7	8	with	comparison	1299:1308	arg1	process					1352:1358	the universally accepted cyanidation process	1315:1358	the universally accepted cyanidation process	1315:1358	This method was found to be easy and safest in comparison with the universally accepted cyanidation process.
36098266	4	9	from	absence	840:846	arg1	degradation					792:802	degradation	792:802	degradation of various dyes in the presence and absence of AuNPs	792:855	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	2	10	theme	gold	564:567	arg1	studies/recovery					580:595	exquisitely gold separation studies/recovery	552:595	exquisitely gold separation studies/recovery	552:595	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	5	11	theme	cytotoxic	962:970	arg1	AuNPs					923:927	AuNPs	923:927	AuNPs	923:927	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	11	theme	cytotoxic	962:970	arg1	agent					972:976	the most active cytotoxic agent	946:976	the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468	946:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	7	12	theme	cyanidation	1340:1350	arg1	process					1352:1358	the universally accepted cyanidation process	1315:1358	the universally accepted cyanidation process	1315:1358	This method was found to be easy and safest in comparison with the universally accepted cyanidation process.
36098266	1	13	theme	green	239:243	arg1	synthesis					245:253	their green synthesis	233:253	their green synthesis	233:253	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	4	14	theme	AuNPs	851:855	arg1	presence					827:834	presence	827:834	presence	827:834	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	4	14	theme	AuNPs	851:855	arg1	absence					840:846	absence	840:846	absence	840:846	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	6	15	theme	detection	1138:1146	arg1	kits					1148:1151	COVID-19 detection kits	1129:1151	COVID-19 detection kits	1129:1151	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	7	16	from	easy	1280:1283	arg1	comparison					1299:1308	comparison	1299:1308	comparison with the universally accepted cyanidation process	1299:1358	This method was found to be easy and safest in comparison with the universally accepted cyanidation process.
36098266	2	17	theme	serum	503:507	arg1	BSA					518:520	BSA	518:520	BSA	518:520	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	17	theme	serum	503:507	arg1	albumin					509:515	bovine serum albumin	496:515	bovine serum albumin (BSA)	496:521	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	0	18	from	AuNPs	48:52	arg1	cycle					25:29	cycle	25:29	cycle	25:29	Economical gold recovery cycle from bio-sensing AuNPs: an application for nanowaste and COVID-19 testing kits.
36098266	2	19	theme	COVID-19	613:620	arg1	kits					630:633	COVID-19 testing kits	613:633	COVID-19 testing kits	613:633	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	20	theme	testing	622:628	arg1	kits					630:633	COVID-19 testing kits	613:633	COVID-19 testing kits	613:633	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	6	21	theme	traces	1106:1111	arg1	recovery					1089:1096	the recovery	1085:1096	the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1085:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	1	22	theme	active	159:164	arg1	recycling					344:352	recycling	344:352	recycling	344:352	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	22	theme	active	159:164	arg1	characterization					256:271	characterization	256:271	characterization	256:271	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	22	theme	active	159:164	arg1	degradation					328:338	degradation	328:338	degradation	328:338	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	22	theme	active	159:164	arg1	compounds					166:174	biologically active compounds	146:174	biologically active compounds: gold nanoparticles (AuNPs) in various shapes	146:220	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	22	theme	active	159:164	arg1	studies					278:284	studies	278:284	studies	278:284	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	22	theme	active	159:164	arg1	synthesis					245:253	their green synthesis	233:253	their green synthesis	233:253	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	2	23	theme	bovine	496:501	arg1	BSA					518:520	BSA	518:520	BSA	518:520	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	23	theme	bovine	496:501	arg1	albumin					509:515	bovine serum albumin	496:515	bovine serum albumin (BSA)	496:521	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	4	24	dep	presence	827:834	arg1	the					823:825	the	823:825	the	823:825	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	4	25	from	presence	827:834	arg1	degradation					792:802	degradation	792:802	degradation of various dyes in the presence and absence of AuNPs	792:855	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	1	26	theme	compounds	166:174	arg1	growth					136:141	the controlled growth	121:141	the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling	121:352	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	0	27	theme	gold	11:14	arg1	recovery					16:23	Economical gold recovery	0:23	Economical gold recovery	0:23	Economical gold recovery cycle from bio-sensing AuNPs: an application for nanowaste and COVID-19 testing kits.
36098266	6	28	theme	pregnancy	1158:1166	arg1	kits					1176:1179	pregnancy testing kits	1158:1179	pregnancy testing kits	1158:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	3	29	theme	AuNPs	705:709	arg1	ability					690:696	The sensing ability	678:696	The sensing ability of the AuNPs with biopolymers	678:726	The sensing ability of the AuNPs with biopolymers was investigated via various analytical techniques.
36098266	0	30	theme	Economical	0:9	arg1	recovery					16:23	Economical gold recovery	0:23	Economical gold recovery	0:23	Economical gold recovery cycle from bio-sensing AuNPs: an application for nanowaste and COVID-19 testing kits.
36098266	2	31	theme	testing	650:656	arg1	kits					658:661	pregnancy testing kits	640:661	pregnancy testing kits	640:661	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	8	32	theme	related	1406:1412	arg1	industries					1414:1423	related industries	1406:1423	related industries	1406:1423	In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth.
36098266	7	33	from	comparison	1299:1308	arg1	easy					1280:1283	easy	1280:1283	easy	1280:1283	This method was found to be easy and safest in comparison with the universally accepted cyanidation process.
36098266	1	34	theme	gold	177:180	arg1	AuNPs					197:201	AuNPs	197:201	AuNPs	197:201	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	34	theme	gold	177:180	arg1	nanoparticles					182:194	gold nanoparticles	177:194	biologically active compounds: gold nanoparticles (AuNPs) in various shapes	146:220	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	2	35	theme	AuNPs	428:432	arg1	mechanisms					414:423	responsive binding mechanisms	395:423	responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits	395:661	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	9	36	theme	trace-level	1587:1597	arg1	investigation					1608:1620	trace-level forensic investigation	1587:1620	trace-level forensic investigation	1587:1620	The methodologies proposed are also beneficial for trace-level forensic investigation.
36098266	2	37	theme	dyes	524:527	arg1	study					541:545	dyes degradation study	524:545	dyes degradation study	524:545	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	38	theme	pregnancy	640:648	arg1	kits					658:661	pregnancy testing kits	640:661	pregnancy testing kits	640:661	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	7	39	theme	accepted	1331:1338	arg1	process					1352:1358	the universally accepted cyanidation process	1315:1358	the universally accepted cyanidation process	1315:1358	This method was found to be easy and safest in comparison with the universally accepted cyanidation process.
36098266	6	40	theme	testing	1168:1174	arg1	kits					1176:1179	pregnancy testing kits	1158:1179	pregnancy testing kits	1158:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	8	41	theme	gold-recycling	1457:1470	arg1	process					1472:1478	the proposed gold-recycling process	1444:1478	the proposed gold-recycling process	1444:1478	In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth.
36098266	2	42	from	studies	384:390	arg1	mechanisms					414:423	responsive binding mechanisms	395:423	responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits	395:661	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	8	43	theme	gold	1382:1385	arg1	makers					1395:1400	small gold jewelry makers	1376:1400	small gold jewelry makers	1376:1400	In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth.
36098266	6	44	from	recovery	1089:1096	arg1	kits					1176:1179	pregnancy testing kits	1158:1179	pregnancy testing kits	1158:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	44	from	recovery	1089:1096	arg1	kits					1148:1151	COVID-19 detection kits	1129:1151	COVID-19 detection kits	1129:1151	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	44	from	recovery	1089:1096	arg1	nanowaste					1118:1126	nanowaste	1118:1126	nanowaste	1118:1126	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	2	45	theme	binding	406:412	arg1	mechanisms					414:423	responsive binding mechanisms	395:423	responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits	395:661	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	0	46	theme	bio-sensing	36:46	arg1	AuNPs					48:52	bio-sensing AuNPs	36:52	bio-sensing AuNPs	36:52	Economical gold recovery cycle from bio-sensing AuNPs: an application for nanowaste and COVID-19 testing kits.
36098266	4	47	theme	solar	896:900	arg1	methods					914:920	stirring, IR, solar, and UV-Vis methods	882:920	stirring, IR, solar, and UV-Vis methods	882:920	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	8	48	theme	small	1376:1380	arg1	makers					1395:1400	small gold jewelry makers	1376:1400	small gold jewelry makers	1376:1400	In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth.
36098266	5	49	theme	active	955:960	arg1	AuNPs					923:927	AuNPs	923:927	AuNPs	923:927	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	49	theme	active	955:960	arg1	agent					972:976	the most active cytotoxic agent	946:976	the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468	946:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	2	50	theme	spectroscopic	361:373	arg1	methods					375:381	spectroscopic methods	361:381	spectroscopic methods	361:381	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	51	theme	responsive	395:404	arg1	mechanisms					414:423	responsive binding mechanisms	395:423	responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits	395:661	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	52	theme	deoxyribonucleic	465:480	arg1	hsDNA					488:492	hsDNA	488:492	hsDNA	488:492	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	52	theme	deoxyribonucleic	465:480	arg1	acid					482:485	sperm deoxyribonucleic acid	459:485	sperm deoxyribonucleic acid (hsDNA)	459:493	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	3	53	theme	analytical	757:766	arg1	techniques					768:777	various analytical techniques	749:777	various analytical techniques	749:777	The sensing ability of the AuNPs with biopolymers was investigated via various analytical techniques.
36098266	4	54	theme	degradation	792:802	arg1	rate					784:787	The rate	780:787	The rate of degradation of various dyes in the presence and absence of AuNPs	780:855	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	2	55	with	AuNPs	428:432	arg1	biopolymers					439:449	biopolymers	439:449	biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste	439:610	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	55	with	AuNPs	428:432	arg1	kits					658:661	pregnancy testing kits	640:661	pregnancy testing kits	640:661	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	55	with	AuNPs	428:432	arg1	kits					630:633	COVID-19 testing kits	613:633	COVID-19 testing kits	613:633	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	3	56	theme	various	749:755	arg1	techniques					768:777	various analytical techniques	749:777	various analytical techniques	749:777	The sensing ability of the AuNPs with biopolymers was investigated via various analytical techniques.
36098266	2	57	theme	sperm	459:463	arg1	hsDNA					488:492	hsDNA	488:492	hsDNA	488:492	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	2	57	theme	sperm	459:463	arg1	acid					482:485	sperm deoxyribonucleic acid	459:485	sperm deoxyribonucleic acid (hsDNA)	459:493	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	8	58	theme	socio-economic	1513:1526	arg1	growth					1528:1533	their socio-economic growth	1507:1533	their socio-economic growth	1507:1533	In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth.
36098266	4	59	theme	various	807:813	arg1	dyes					815:818	various dyes	807:818	various dyes in the presence and absence of AuNPs	807:855	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	8	60	theme	jewelry	1387:1393	arg1	makers					1395:1400	small gold jewelry makers	1376:1400	small gold jewelry makers	1376:1400	In the future, small gold jewelry makers and related industries would benefit from the proposed gold-recycling process and it might contribute to their socio-economic growth.
36098266	4	61	theme	dyes	815:818	arg1	degradation					792:802	degradation	792:802	degradation of various dyes in the presence and absence of AuNPs	792:855	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	6	62	from	nanowaste	1118:1126	arg1	recovery					1089:1096	the recovery	1085:1096	the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1085:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	62	from	nanowaste	1118:1126	arg1	traces					1106:1111	gold traces	1101:1111	gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1101:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	4	63	theme	IR	892:893	arg1	methods					914:920	stirring, IR, solar, and UV-Vis methods	882:920	stirring, IR, solar, and UV-Vis methods	882:920	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	5	64	theme	human	986:990	arg1	MDAMB-468					1035:1043	MDAMB-468	1035:1043	MDAMB-468	1035:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	64	theme	human	986:990	arg1	lines					1011:1015	human breast cancer cell lines	986:1015	human breast cancer cell lines such as MCF-7 and MDAMB-468	986:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	64	theme	human	986:990	arg1	MCF-7					1025:1029	MCF-7	1025:1029	MCF-7	1025:1029	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	1	65	dep	compounds	166:174	arg1	AuNPs					197:201	AuNPs	197:201	AuNPs	197:201	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	65	dep	compounds	166:174	arg1	nanoparticles					182:194	gold nanoparticles	177:194	biologically active compounds: gold nanoparticles (AuNPs) in various shapes	146:220	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	66	from	nanoparticles	182:194	arg1	shapes					215:220	various shapes	207:220	various shapes	207:220	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	6	67	theme	economical	1062:1071	arg1	process					1073:1079	an economical process	1059:1079	an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1059:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	1	68	theme	applications	295:306	arg1	characterization					256:271	characterization	256:271	characterization	256:271	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	68	theme	applications	295:306	arg1	studies					278:284	studies	278:284	studies	278:284	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	1	68	theme	applications	295:306	arg1	synthesis					245:253	their green synthesis	233:253	their green synthesis	233:253	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	4	69	theme	stirring	882:889	arg1	methods					914:920	stirring, IR, solar, and UV-Vis methods	882:920	stirring, IR, solar, and UV-Vis methods	882:920	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	5	70	theme	breast	992:997	arg1	MDAMB-468					1035:1043	MDAMB-468	1035:1043	MDAMB-468	1035:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	70	theme	breast	992:997	arg1	lines					1011:1015	human breast cancer cell lines	986:1015	human breast cancer cell lines such as MCF-7 and MDAMB-468	986:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	70	theme	breast	992:997	arg1	MCF-7					1025:1029	MCF-7	1025:1029	MCF-7	1025:1029	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	4	71	from	degradation	792:802	arg1	presence					827:834	presence	827:834	presence	827:834	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	4	71	from	degradation	792:802	arg1	absence					840:846	absence	840:846	absence	840:846	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	2	72	theme	degradation	529:539	arg1	study					541:545	dyes degradation study	524:545	dyes degradation study	524:545	Using spectroscopic methods, studies on responsive binding mechanisms of AuNPs with biopolymers herring sperm deoxyribonucleic acid (hsDNA), bovine serum albumin (BSA), dyes degradation study, and exquisitely gold separation studies/recovery from nanowaste, COVID-19 testing kits, and pregnancy testing kits are discussed.
36098266	5	73	theme	cancer	999:1004	arg1	MDAMB-468					1035:1043	MDAMB-468	1035:1043	MDAMB-468	1035:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	73	theme	cancer	999:1004	arg1	lines					1011:1015	human breast cancer cell lines	986:1015	human breast cancer cell lines such as MCF-7 and MDAMB-468	986:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	73	theme	cancer	999:1004	arg1	MCF-7					1025:1029	MCF-7	1025:1029	MCF-7	1025:1029	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	4	74	from	dyes	815:818	arg1	presence					827:834	presence	827:834	presence	827:834	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	4	74	from	dyes	815:818	arg1	absence					840:846	absence	840:846	absence	840:846	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36098266	1	75	theme	various	207:213	arg1	shapes					215:220	various shapes	207:220	various shapes	207:220	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	5	76	theme	cell	1006:1009	arg1	MDAMB-468					1035:1043	MDAMB-468	1035:1043	MDAMB-468	1035:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	76	theme	cell	1006:1009	arg1	lines					1011:1015	human breast cancer cell lines	986:1015	human breast cancer cell lines such as MCF-7 and MDAMB-468	986:1043	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	5	76	theme	cell	1006:1009	arg1	MCF-7					1025:1029	MCF-7	1025:1029	MCF-7	1025:1029	AuNPs were found to be the most active cytotoxic agent against human breast cancer cell lines such as MCF-7 and MDAMB-468.
36098266	6	77	theme	COVID-19	1129:1136	arg1	kits					1148:1151	COVID-19 detection kits	1129:1151	COVID-19 detection kits	1129:1151	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	6	78	theme	gold	1101:1104	arg1	traces					1106:1111	gold traces	1101:1111	gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits	1101:1179	Furthermore, an economical process for the recovery of gold traces from nanowaste, COVID-19 detection kits, and pregnancy testing kits was developed using inexpensive and eco-friendly α-cyclodextrin sugar.
36098266	1	79	theme	controlled	125:134	arg1	growth					136:141	the controlled growth	121:141	the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling	121:352	We report the controlled growth of biologically active compounds: gold nanoparticles (AuNPs) in various shapes, including their green synthesis, characterization, and studies of their applications towards biological, degradation and recycling.
36098266	3	80	with	AuNPs	705:709	arg1	biopolymers					716:726	biopolymers	716:726	biopolymers	716:726	The sensing ability of the AuNPs with biopolymers was investigated via various analytical techniques.
36098266	9	81	theme	forensic	1599:1606	arg1	investigation					1608:1620	trace-level forensic investigation	1587:1620	trace-level forensic investigation	1587:1620	The methodologies proposed are also beneficial for trace-level forensic investigation.
36098266	4	82	theme	UV-Vis	907:912	arg1	methods					914:920	stirring, IR, solar, and UV-Vis methods	882:920	stirring, IR, solar, and UV-Vis methods	882:920	The rate of degradation of various dyes in the presence and absence of AuNPs was studied by deploying stirring, IR, solar, and UV-Vis methods.
36718822	6	0	theme	responses	853:861	arg1	assessment					830:839	the assessment	826:839	the assessment of cellular responses	826:861	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	7	1	theme	Gel-Al	905:910	arg1	group					918:922	Gel-Al alone group	905:922	Gel-Al alone group	905:922	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	2	theme	factor	1154:1159	arg1	expression					1073:1082	the expression	1069:1082	the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31	1069:1231	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	1	3	theme	diabetic	191:198	arg1	patients					200:207	diabetic patients	191:207	diabetic patients	191:207	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	7	4	theme	platelet-derived	1130:1145	arg1	PDGF					1162:1165	PDGF	1162:1165	PDGF	1162:1165	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	4	theme	platelet-derived	1130:1145	arg1	factor					1154:1159	platelet-derived growth factor	1130:1159	platelet-derived growth factor (PDGF)	1130:1166	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	2	5	theme	diabetic	301:308	arg1	repair					316:321	diabetic wound repair	301:321	diabetic wound repair	301:321	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	10	6	theme	ASC	1634:1636	arg1	system					1638:1643	The Gel-Al hydrogel-based ASC system	1608:1643	The Gel-Al hydrogel-based ASC system	1608:1643	The Gel-Al hydrogel-based ASC system therefore represents an innovative therapeutic strategy for diabetic wound repair.
36718822	4	7	theme	Full-thickness	460:473	arg1	wounds					485:490	Full-thickness cutaneous wounds	460:490	METHODS Full-thickness cutaneous wounds	452:490	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	10	8	theme	Gel-Al	1612:1617	arg1	system					1638:1643	The Gel-Al hydrogel-based ASC system	1608:1643	The Gel-Al hydrogel-based ASC system	1608:1643	The Gel-Al hydrogel-based ASC system therefore represents an innovative therapeutic strategy for diabetic wound repair.
36718822	7	9	theme	growth	1108:1113	arg1	VEGF					1123:1126	VEGF	1123:1126	VEGF	1123:1126	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	9	theme	growth	1108:1113	arg1	factor					1115:1120	vascular endothelial growth factor	1087:1120	vascular endothelial growth factor (VEGF)	1087:1127	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	1	10	theme	Chronic	136:142	arg1	wounds					155:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	1	10	theme	Chronic	136:142	arg1	complication					175:186	a common complication	166:186	a common complication in diabetic patients	166:207	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	8	11	theme	interleukin-13	1450:1463	arg1	beta1					1386:1390	transforming growth factor beta1	1359:1390	transforming growth factor beta1 (TGFβ1)	1359:1398	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	11	theme	interleukin-13	1450:1463	arg1	expression					1473:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	12	theme	interleukin-1β	1314:1327	arg1	expression					1337:1346	interleukin-1β (IL-1β) expression	1314:1346	interleukin-1β (IL-1β) expression	1314:1346	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	6	13	theme	Masson	695:700	arg1	staining					702:709	Masson staining	695:709	Masson staining	695:709	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	7	14	theme	vascular	1087:1094	arg1	VEGF					1123:1126	VEGF	1123:1126	VEGF	1123:1126	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	14	theme	vascular	1087:1094	arg1	factor					1115:1120	vascular endothelial growth factor	1087:1120	vascular endothelial growth factor (VEGF)	1087:1127	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	8	15	theme	IL-1β	1330:1334	arg1	expression					1337:1346	interleukin-1β (IL-1β) expression	1314:1346	interleukin-1β (IL-1β) expression	1314:1346	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	4	16	theme	streptozotocin-induced	508:529	arg1	rats					540:543	streptozotocin-induced diabetic rats	508:543	streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs	508:601	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	0	17	theme	wound	93:97	arg1	healing					99:105	wound healing	93:105	wound healing	93:105	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	5	18	theme	wound	634:638	arg1	healing					640:646	wound healing	634:646	wound healing	634:646	Hydrogel biocompatibility and wound healing were analyzed.
36718822	7	19	theme	CD31	1228:1231	arg1	expression					1073:1082	the expression	1069:1082	the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31	1069:1231	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	9	20	theme	Gel-Al	1539:1544	arg1	hydrogels					1546:1554	CONCLUSIONS Gel-Al hydrogels	1527:1554	CONCLUSIONS Gel-Al hydrogels loaded with ASCs	1527:1571	CONCLUSIONS Gel-Al hydrogels loaded with ASCs accelerate diabetic wound healing.
36718822	8	21	theme	M2	1499:1500	arg1	polarization					1513:1524	M2 macrophage polarization	1499:1524	M2 macrophage polarization	1499:1524	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	22	theme	growth	1372:1377	arg1	interleukin-10					1401:1414	interleukin-10	1401:1414	interleukin-10 (IL-10)	1401:1422	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	22	theme	growth	1372:1377	arg1	beta1					1386:1390	transforming growth factor beta1	1359:1390	transforming growth factor beta1 (TGFβ1)	1359:1398	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	22	theme	growth	1372:1377	arg1	expression					1473:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	22	theme	growth	1372:1377	arg1	TGFβ1					1393:1397	TGFβ1	1393:1397	TGFβ1	1393:1397	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	3	23	theme	wound	437:441	arg1	healing					443:449	diabetic wound healing	428:449	diabetic wound healing	428:449	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	7	24	theme	cell	1216:1219	arg1	CD31					1228:1231	endothelial cell marker CD31	1204:1231	endothelial cell marker CD31	1204:1231	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	0	25	theme	adipose-derived	46:60	arg1	cells					79:83	adipose-derived mesenchymal stem cells	46:83	adipose-derived mesenchymal stem cells	46:83	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	3	26	theme	alginate/gelatin	381:396	arg1	hydrogel					407:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	0	27	theme	Sodium	0:5	arg1	hydrogels					24:32	Sodium alginate/gelatin hydrogels	0:32	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells	0:83	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	10	28	theme	diabetic	1705:1712	arg1	repair					1720:1725	diabetic wound repair	1705:1725	diabetic wound repair	1705:1725	The Gel-Al hydrogel-based ASC system therefore represents an innovative therapeutic strategy for diabetic wound repair.
36718822	9	29	theme	diabetic	1584:1591	arg1	healing					1599:1605	diabetic wound healing	1584:1605	diabetic wound healing	1584:1605	CONCLUSIONS Gel-Al hydrogels loaded with ASCs accelerate diabetic wound healing.
36718822	3	30	theme	Gel-Al	399:404	arg1	hydrogel					407:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	0	31	theme	mesenchymal	62:72	arg1	cells					79:83	adipose-derived mesenchymal stem cells	46:83	adipose-derived mesenchymal stem cells	46:83	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	2	32	theme	stem	238:241	arg1	ASCs					250:253	ASCs	250:253	ASCs	250:253	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	2	32	theme	stem	238:241	arg1	cells					243:247	Adipose-derived mesenchymal stem cells	210:247	Adipose-derived mesenchymal stem cells (ASCs)	210:254	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	6	33	theme	real-time	793:801	arg1	PCR					803:805	quantitative real-time PCR	780:805	quantitative real-time PCR	780:805	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	7	34	theme	ASCs	955:958	arg1	combination					929:939	the combination	925:939	the combination of Gel-Al and ASCs	925:958	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	2	35	theme	Adipose-derived	210:224	arg1	ASCs					250:253	ASCs	250:253	ASCs	250:253	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	2	35	theme	Adipose-derived	210:224	arg1	cells					243:247	Adipose-derived mesenchymal stem cells	210:247	Adipose-derived mesenchymal stem cells (ASCs)	210:254	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	8	36	theme	interleukin-4	1425:1437	arg1	beta1					1386:1390	transforming growth factor beta1	1359:1390	transforming growth factor beta1 (TGFβ1)	1359:1398	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	36	theme	interleukin-4	1425:1437	arg1	expression					1473:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	7	37	theme	Gel-Al	944:949	arg1	combination					929:939	the combination	925:939	the combination of Gel-Al and ASCs	925:958	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	0	38	theme	stem	74:77	arg1	cells					79:83	adipose-derived mesenchymal stem cells	46:83	adipose-derived mesenchymal stem cells	46:83	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	4	39	dep	METHODS	452:458	arg1	wounds					485:490	Full-thickness cutaneous wounds	460:490	METHODS Full-thickness cutaneous wounds	452:490	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	7	40	theme	growth	1147:1152	arg1	PDGF					1162:1165	PDGF	1162:1165	PDGF	1162:1165	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	40	theme	growth	1147:1152	arg1	factor					1154:1159	platelet-derived growth factor	1130:1159	platelet-derived growth factor (PDGF)	1130:1166	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	6	41	theme	cellular	844:851	arg1	responses					853:861	cellular responses	844:861	cellular responses	844:861	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	2	42	theme	essential	283:291	arg1	role					293:296	an essential role	280:296	an essential role	280:296	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	7	43	theme	growth	1179:1184	arg1	factor					1186:1191	epidermal growth factor	1169:1191	epidermal growth factor (EGF)	1169:1197	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	43	theme	growth	1179:1184	arg1	EGF					1194:1196	EGF	1194:1196	EGF	1194:1196	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	0	44	link	adipose-derived	46:60	arg1	cells					79:83	adipose-derived mesenchymal stem cells	46:83	adipose-derived mesenchymal stem cells	46:83	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	8	45	theme	Gel-Al	1263:1268	arg1	combination					1248:1258	the combination	1244:1258	the combination of Gel-Al and ASCs	1244:1277	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	2	46	theme	wound	310:314	arg1	repair					316:321	diabetic wound repair	301:321	diabetic wound repair	301:321	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	7	47	theme	tissue	1008:1013	arg1	regeneration					1015:1026	granulation tissue regeneration	996:1026	granulation tissue regeneration	996:1026	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	8	48	theme	ASCs	1274:1277	arg1	combination					1248:1258	the combination	1244:1258	the combination of Gel-Al and ASCs	1244:1277	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	7	49	theme	factor	1115:1120	arg1	expression					1073:1082	the expression	1069:1082	the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31	1069:1231	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	4	50	theme	cutaneous	475:483	arg1	wounds					485:490	Full-thickness cutaneous wounds	460:490	METHODS Full-thickness cutaneous wounds	452:490	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	10	51	theme	hydrogel-based	1619:1632	arg1	system					1638:1643	The Gel-Al hydrogel-based ASC system	1608:1643	The Gel-Al hydrogel-based ASC system	1608:1643	The Gel-Al hydrogel-based ASC system therefore represents an innovative therapeutic strategy for diabetic wound repair.
36718822	7	52	theme	marker	1221:1226	arg1	CD31					1228:1231	endothelial cell marker CD31	1204:1231	endothelial cell marker CD31	1204:1231	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	53	theme	endothelial	1096:1106	arg1	VEGF					1123:1126	VEGF	1123:1126	VEGF	1123:1126	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	53	theme	endothelial	1096:1106	arg1	factor					1115:1120	vascular endothelial growth factor	1087:1120	vascular endothelial growth factor (VEGF)	1087:1127	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	1	54	theme	BACKGROUND	125:134	arg1	wounds					155:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	1	54	theme	BACKGROUND	125:134	arg1	complication					175:186	a common complication	166:186	a common complication in diabetic patients	166:207	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	1	55	theme	refractory	144:153	arg1	wounds					155:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	1	55	theme	refractory	144:153	arg1	complication					175:186	a common complication	166:186	a common complication in diabetic patients	166:207	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	4	56	with	treatment	554:562	arg1	hydrogels					576:584	Gel-Al hydrogels	569:584	Gel-Al hydrogels loaded with ASCs	569:601	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	7	57	theme	control	888:894	arg1	group					896:900	the control group	884:900	the control group	884:900	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	4	58	theme	diabetic	531:538	arg1	rats					540:543	streptozotocin-induced diabetic rats	508:543	streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs	508:601	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	6	59	theme	eosin	679:683	arg1	staining					685:692	Hematoxylin and eosin staining	663:692	Hematoxylin and eosin staining	663:692	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	7	60	theme	collagen	1032:1039	arg1	deposition					1041:1050	collagen deposition	1032:1050	collagen deposition	1032:1050	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	1	61	theme	common	168:173	arg1	wounds					155:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds	125:160	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	1	61	theme	common	168:173	arg1	complication					175:186	a common complication	166:186	a common complication in diabetic patients	166:207	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	4	62	theme	prior	545:549	arg1	rats					540:543	streptozotocin-induced diabetic rats	508:543	streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs	508:601	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	6	63	theme	Hematoxylin	663:673	arg1	staining					685:692	Hematoxylin and eosin staining	663:692	Hematoxylin and eosin staining	663:692	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	0	64	theme	diabetic	110:117	arg1	rats					119:122	diabetic rats	110:122	diabetic rats	110:122	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	1	65	from	complication	175:186	arg1	patients					200:207	diabetic patients	191:207	diabetic patients	191:207	BACKGROUND Chronic refractory wounds are a common complication in diabetic patients.
36718822	9	66	theme	CONCLUSIONS	1527:1537	arg1	hydrogels					1546:1554	CONCLUSIONS Gel-Al hydrogels	1527:1554	CONCLUSIONS Gel-Al hydrogels loaded with ASCs	1527:1571	CONCLUSIONS Gel-Al hydrogels loaded with ASCs accelerate diabetic wound healing.
36718822	3	67	theme	ASCs	365:368	arg1	composite					352:360	a composite	350:360	a composite of ASCs	350:368	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	3	67	theme	ASCs	365:368	arg1	hydrogel					407:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	3	67	theme	ASCs	365:368	arg1	ASCs					365:368	ASCs	365:368	ASCs	365:368	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	8	68	theme	macrophage	1502:1511	arg1	polarization					1513:1524	M2 macrophage polarization	1499:1524	M2 macrophage polarization	1499:1524	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	6	69	dep	enzyme-linked	732:744	arg1	immunosorbent					746:758	immunosorbent	746:758	immunosorbent	746:758	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	7	70	theme	endothelial	1204:1214	arg1	CD31					1228:1231	endothelial cell marker CD31	1204:1231	endothelial cell marker CD31	1204:1231	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	71	theme	granulation	996:1006	arg1	regeneration					1015:1026	granulation tissue regeneration	996:1026	granulation tissue regeneration	996:1026	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	8	72	theme	transforming	1359:1370	arg1	interleukin-10					1401:1414	interleukin-10	1401:1414	interleukin-10 (IL-10)	1401:1422	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	72	theme	transforming	1359:1370	arg1	beta1					1386:1390	transforming growth factor beta1	1359:1390	transforming growth factor beta1 (TGFβ1)	1359:1398	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	72	theme	transforming	1359:1370	arg1	expression					1473:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	72	theme	transforming	1359:1370	arg1	TGFβ1					1393:1397	TGFβ1	1393:1397	TGFβ1	1393:1397	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	7	73	link	platelet-derived	1130:1145	arg1	PDGF					1162:1165	PDGF	1162:1165	PDGF	1162:1165	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	73	link	platelet-derived	1130:1145	arg1	factor					1154:1159	platelet-derived growth factor	1130:1159	platelet-derived growth factor (PDGF)	1130:1166	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	0	74	theme	alginate/gelatin	7:22	arg1	hydrogels					24:32	Sodium alginate/gelatin hydrogels	0:32	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells	0:83	Sodium alginate/gelatin hydrogels loaded with adipose-derived mesenchymal stem cells promote wound healing in diabetic rats.
36718822	6	75	link	enzyme-linked	732:744	arg1	assays					760:765	enzyme-linked immunosorbent assays	732:765	enzyme-linked immunosorbent assays (ELISA)	732:773	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	6	75	link	enzyme-linked	732:744	arg1	ELISA					768:772	ELISA	768:772	ELISA	768:772	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	5	76	theme	Hydrogel	604:611	arg1	biocompatibility					613:628	Hydrogel biocompatibility	604:628	Hydrogel biocompatibility	604:628	Hydrogel biocompatibility and wound healing were analyzed.
36718822	8	77	theme	factor	1379:1384	arg1	interleukin-10					1401:1414	interleukin-10	1401:1414	interleukin-10 (IL-10)	1401:1422	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	77	theme	factor	1379:1384	arg1	beta1					1386:1390	transforming growth factor beta1	1359:1390	transforming growth factor beta1 (TGFβ1)	1359:1398	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	77	theme	factor	1379:1384	arg1	expression					1473:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	interleukin-4 (IL-4) and interleukin-13 (IL-13) expression	1425:1482	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	8	77	theme	factor	1379:1384	arg1	TGFβ1					1393:1397	TGFβ1	1393:1397	TGFβ1	1393:1397	Moreover, the combination of Gel-Al and ASCs decreased interleukin-6 (IL-6) and interleukin-1β (IL-1β) expression, increased transforming growth factor beta1 (TGFβ1), interleukin-10 (IL-10), interleukin-4 (IL-4) and interleukin-13 (IL-13) expression, and increased M2 macrophage polarization.
36718822	6	78	theme	enzyme-linked	732:744	arg1	assays					760:765	enzyme-linked immunosorbent assays	732:765	enzyme-linked immunosorbent assays (ELISA)	732:773	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	6	78	theme	enzyme-linked	732:744	arg1	ELISA					768:772	ELISA	768:772	ELISA	768:772	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	3	79	theme	diabetic	428:435	arg1	healing					443:449	diabetic wound healing	428:449	diabetic wound healing	428:449	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	7	80	theme	wound	969:973	arg1	closure					975:981	wound closure	969:981	wound closure	969:981	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	3	81	theme	sodium	374:379	arg1	hydrogel					407:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	sodium alginate/gelatin (Gel-Al) hydrogel	374:414	AIMS To determine whether a composite of ASCs and sodium alginate/gelatin (Gel-Al) hydrogel can promote diabetic wound healing.
36718822	2	82	link	Adipose-derived	210:224	arg1	ASCs					250:253	ASCs	250:253	ASCs	250:253	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	2	82	link	Adipose-derived	210:224	arg1	cells					243:247	Adipose-derived mesenchymal stem cells	210:247	Adipose-derived mesenchymal stem cells (ASCs)	210:254	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	7	83	theme	factor	1186:1191	arg1	expression					1073:1082	the expression	1069:1082	the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31	1069:1231	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	9	84	theme	wound	1593:1597	arg1	healing					1599:1605	diabetic wound healing	1584:1605	diabetic wound healing	1584:1605	CONCLUSIONS Gel-Al hydrogels loaded with ASCs accelerate diabetic wound healing.
36718822	10	85	theme	innovative	1669:1678	arg1	strategy					1692:1699	an innovative therapeutic strategy	1666:1699	an innovative therapeutic strategy for diabetic wound repair	1666:1725	The Gel-Al hydrogel-based ASC system therefore represents an innovative therapeutic strategy for diabetic wound repair.
36718822	10	86	theme	therapeutic	1680:1690	arg1	strategy					1692:1699	an innovative therapeutic strategy	1666:1699	an innovative therapeutic strategy for diabetic wound repair	1666:1725	The Gel-Al hydrogel-based ASC system therefore represents an innovative therapeutic strategy for diabetic wound repair.
36718822	7	87	theme	epidermal	1169:1177	arg1	factor					1186:1191	epidermal growth factor	1169:1191	epidermal growth factor (EGF)	1169:1197	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	7	87	theme	epidermal	1169:1177	arg1	EGF					1194:1196	EGF	1194:1196	EGF	1194:1196	RESULTS Compared to the control group or Gel-Al alone group, the combination of Gel-Al and ASCs promoted wound closure, facilitated granulation tissue regeneration and collagen deposition, and upregulated the expression of vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and endothelial cell marker CD31.
36718822	4	88	theme	Gel-Al	569:574	arg1	hydrogels					576:584	Gel-Al hydrogels	569:584	Gel-Al hydrogels loaded with ASCs	569:601	METHODS Full-thickness cutaneous wounds were created in streptozotocin-induced diabetic rats prior to treatment with Gel-Al hydrogels loaded with ASCs.
36718822	2	89	theme	mesenchymal	226:236	arg1	ASCs					250:253	ASCs	250:253	ASCs	250:253	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	2	89	theme	mesenchymal	226:236	arg1	cells					243:247	Adipose-derived mesenchymal stem cells	210:247	Adipose-derived mesenchymal stem cells (ASCs)	210:254	Adipose-derived mesenchymal stem cells (ASCs) have been shown to play an essential role in diabetic wound repair.
36718822	6	90	theme	quantitative	780:791	arg1	PCR					803:805	quantitative real-time PCR	780:805	quantitative real-time PCR	780:805	Hematoxylin and eosin staining, Masson staining, immunofluorescence, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time PCR were performed for the assessment of cellular responses.
36718822	10	91	theme	wound	1714:1718	arg1	repair					1720:1725	diabetic wound repair	1705:1725	diabetic wound repair	1705:1725	The Gel-Al hydrogel-based ASC system therefore represents an innovative therapeutic strategy for diabetic wound repair.
36701335	10	0	theme	serum	1665:1669	arg1	triglycerides					1671:1683	fasting serum triglycerides	1657:1683	fasting serum triglycerides	1657:1683	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	11	1	from	similar	1754:1760	arg1	sexes					1770:1774	both sexes	1765:1774	both sexes	1765:1774	Effects were similar in both sexes and not observed in the HCr group.
36701335	4	2	theme	young	778:782	arg1	age					790:792	young adult age	778:792	young adult age	778:792	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	9	3	from	min	1508:1510	arg1	insulin					1487:1493	insulin	1487:1493	insulin	1487:1493	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	9	3	from	min	1508:1510	arg1	insulin					1475:1481	fasting plasma insulin	1460:1481	fasting plasma insulin	1460:1481	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	8	4	theme	Day	1303:1305	arg1	onwards					1310:1316	Day 56 onwards	1303:1316	Day 56 onwards	1303:1316	Compared to the NCr group, the LCr group weighed more (p<0.01) and consumed more energy (food) from Day 56 onwards, but food efficiency was unaffected.
36701335	5	5	theme	±	1013:1013	arg1	mg/kg					1020:1024	1.20 ± 0.11 mg/kg	1008:1024	1.20 ± 0.11 mg/kg	1008:1024	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	5	theme	±	1013:1013	arg1	NCr					1003:1005	NCr	1003:1005	NCr	1003:1005	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	7	6	theme	body	1133:1136	arg1	weights					1138:1144	body weights	1133:1144	body weights	1133:1144	At baseline, body weights and composition were similar (p≥0.05) among diet groups.
36701335	4	7	theme	levels	582:587	arg1	effects					561:567	the effects	557:567	the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats	557:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	9	8	theme	plasma	1440:1445	arg1	glucose					1447:1453	plasma glucose	1440:1453	plasma glucose	1440:1453	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	4	9	theme	adult	784:788	arg1	age					790:792	young adult age	778:792	young adult age	778:792	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	10	theme	dietary	592:598	arg1	Cr					600:601	dietary Cr	592:601	dietary Cr	592:601	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	0	11	theme	young	185:189	arg1	age					197:199	young adult age	185:199	young adult age	185:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	9	12	theme	LCr	1544:1546	arg1	group					1548:1552	the LCr group	1540:1552	the LCr group	1540:1552	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	5	13	dep	=	883:883	arg1	males					888:892	10 males	885:892	10 males	885:892	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	13	dep	=	883:883	arg1	females/group					901:913	10 females/group	898:913	10 females/group	898:913	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	0	14	theme	male	99:102	arg1	rats					115:118	male and female rats	99:118	male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age	99:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	12	15	theme	common	1876:1881	arg1	parameters					1865:1874	metabolic parameters	1855:1874	metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr	1855:2006	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	10	16	theme	LCr	1704:1706	arg1	group					1708:1712	the LCr group	1700:1712	the LCr group	1700:1712	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	0	17	theme	female	108:113	arg1	rats					115:118	male and female rats	99:118	male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age	99:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	3	18	theme	rodent	523:528	arg1	diets					530:534	rodent diets	523:534	rodent diets	523:534	Cr is recommended at 1 mg per kg in rodent diets.
36701335	9	19	theme	glucose	1373:1379	arg1	challenge					1381:1389	an oral glucose challenge	1365:1389	an oral glucose challenge (Day 77)	1365:1398	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	9	19	theme	glucose	1373:1379	arg1	Day					1392:1394	Day 77	1392:1397	Day 77	1392:1397	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	12	20	theme	chronic	1886:1892	arg1	diseases					1894:1901	chronic diseases	1886:1901	chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr	1886:2006	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	4	21	theme	body	619:622	arg1	composition					624:634	body composition	619:634	body composition	619:634	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	0	22	from	insulin	88:94	arg1	rats					115:118	male and female rats	99:118	male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age	99:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	5	23	theme	±	1046:1046	arg1	HCr					1036:1038	HCr	1036:1038	HCr	1036:1038	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	23	theme	±	1046:1046	arg1	mg/kg					1053:1057	9.15 ± 0.65 mg/kg	1041:1057	9.15 ± 0.65 mg/kg	1041:1057	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	7	24	from	baseline	1123:1130	arg1	similar					1167:1173	similar	1167:1173	similar	1167:1173	At baseline, body weights and composition were similar (p≥0.05) among diet groups.
36701335	2	25	theme	feeding	437:443	arg1	quantities					449:458	feeding low quantities	437:458	feeding low quantities	437:458	Cr is listed as a potentially beneficial element for rodents based on studies that show feeding low quantities affect glucose metabolism.
36701335	0	26	from	triglycerides	70:82	arg1	rats					115:118	male and female rats	99:118	male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age	99:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	3	27	from	mg	510:511	arg1	diets					530:534	rodent diets	523:534	rodent diets	523:534	Cr is recommended at 1 mg per kg in rodent diets.
36701335	5	28	from	days	922:925	arg1	rats					875:878	Sprague-Dawley CD rats	857:878	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age	857:932	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	29	theme	low	965:967	arg1	diets					1063:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	12	30	from	common	1876:1881	arg1	diseases					1894:1901	chronic diseases	1886:1901	chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr	1886:2006	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	10	31	theme	accrued	1622:1628	arg1	fat					1635:1637	accrued body fat	1622:1637	accrued body fat from baseline	1622:1651	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	4	32	from	peripuberty	763:773	arg1	background					801:810	the background	797:810	the background of a moderately high-fat, high-sucrose diet	797:854	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	33	from	parameters	682:691	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	2	34	theme	glucose	467:473	arg1	metabolism					475:484	glucose metabolism	467:484	glucose metabolism	467:484	Cr is listed as a potentially beneficial element for rodents based on studies that show feeding low quantities affect glucose metabolism.
36701335	12	35	theme	low	1832:1834	arg1	Cr					1844:1845	low dietary Cr	1832:1845	low dietary Cr	1832:1845	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	1	36	theme	chromium	295:302	arg1	intakes					304:310	chromium intakes	295:310	chromium intakes	295:310	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	5	37	theme	Sprague-Dawley	857:870	arg1	rats					875:878	Sprague-Dawley CD rats	857:878	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age	857:932	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	4	38	theme	diet	851:854	arg1	background					801:810	the background	797:810	the background of a moderately high-fat, high-sucrose diet	797:854	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	0	39	from	fat	35:37	arg1	rats					115:118	male and female rats	99:118	male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age	99:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	10	40	from	baseline	1644:1651	arg1	fat					1617:1619	whole-body fat	1606:1619	whole-body fat	1606:1619	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	10	40	from	baseline	1644:1651	arg1	fat					1635:1637	accrued body fat	1622:1637	accrued body fat from baseline	1622:1651	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	10	40	from	baseline	1644:1651	arg1	triglycerides					1671:1683	fasting serum triglycerides	1657:1683	fasting serum triglycerides	1657:1683	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	4	41	from	rats	744:747	arg1	weight					611:616	body weight	606:616	body weight	606:616	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	41	from	rats	744:747	arg1	composition					624:634	body composition	619:634	body composition	619:634	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	41	from	rats	744:747	arg1	intake					644:649	energy intake	637:649	energy intake	637:649	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	41	from	rats	744:747	arg1	efficiency					657:666	food efficiency	652:666	food efficiency	652:666	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	41	from	rats	744:747	arg1	parameters					682:691	metabolic parameters	672:691	metabolic parameters	672:691	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	0	42	theme	body	30:33	arg1	fat					35:37	body fat	30:37	body fat	30:37	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	11	43	dep	similar	1754:1760	arg1	observed					1784:1791	observed	1784:1791	observed in the HCr group	1784:1808	Effects were similar in both sexes and not observed in the HCr group.
36701335	12	44	theme	metabolic	1855:1863	arg1	parameters					1865:1874	metabolic parameters	1855:1874	metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr	1855:2006	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	4	45	from	intake	644:649	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	8	46	theme	LCr	1234:1236	arg1	group					1238:1242	the LCr group	1230:1242	the LCr group	1230:1242	Compared to the NCr group, the LCr group weighed more (p<0.01) and consumed more energy (food) from Day 56 onwards, but food efficiency was unaffected.
36701335	4	47	theme	food	652:655	arg1	efficiency					657:666	food efficiency	652:666	food efficiency	652:666	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	5	48	theme	Cr	1060:1061	arg1	diets					1063:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	0	49	theme	chromium	6:13	arg1	diet					15:18	A low chromium diet	0:18	A low chromium diet	0:18	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	5	50	theme	normal	995:1000	arg1	diets					1063:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	12	51	theme	Cr	2005:2006	arg1	effects					1994:2000	the nutritional and/or pharmacological effects	1955:2000	the nutritional and/or pharmacological effects of Cr	1955:2006	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	9	52	theme	plasma	1468:1473	arg1	insulin					1475:1481	fasting plasma insulin	1460:1481	fasting plasma insulin	1460:1481	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	0	53	theme	energy	40:45	arg1	intake					47:52	energy intake	40:52	energy intake	40:52	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	10	54	theme	NCr	1730:1732	arg1	group					1734:1738	the NCr group	1726:1738	the NCr group	1726:1738	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	1	55	theme	insulin	267:273	arg1	action					257:262	the action	253:262	the action of insulin	253:273	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	0	56	from	peripuberty	170:180	arg1	diet					160:163	a moderately high-fat, high-sucrose diet	124:163	a moderately high-fat, high-sucrose diet from peripuberty to young adult age	124:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	11	57	from	sexes	1770:1774	arg1	similar					1754:1760	similar	1754:1760	similar	1754:1760	Effects were similar in both sexes and not observed in the HCr group.
36701335	4	58	theme	lipid	696:700	arg1	metabolism					714:723	lipid and glucose metabolism	696:723	metabolism	714:723	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	59	from	composition	624:634	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	2	60	dep	show	432:435	arg1	affect					460:465	affect	460:465	show feeding low quantities affect glucose metabolism	432:484	Cr is listed as a potentially beneficial element for rodents based on studies that show feeding low quantities affect glucose metabolism.
36701335	12	61	theme	clinical	1929:1936	arg1	trials					1938:1943	clinical trials	1929:1943	clinical trials	1929:1943	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	4	62	theme	glucose	706:712	arg1	metabolism					714:723	lipid and glucose metabolism	696:723	metabolism	714:723	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	1	63	theme	Trivalent	202:210	arg1	Cr					222:223	Cr	222:223	Cr	222:223	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	1	63	theme	Trivalent	202:210	arg1	chromium					212:219	Trivalent chromium	202:219	Trivalent chromium (Cr)	202:224	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	7	64	theme	diet	1190:1193	arg1	groups					1195:1200	diet groups	1190:1200	diet groups	1190:1200	At baseline, body weights and composition were similar (p≥0.05) among diet groups.
36701335	8	65	theme	food	1323:1326	arg1	efficiency					1328:1337	food efficiency	1323:1337	food efficiency	1323:1337	Compared to the NCr group, the LCr group weighed more (p<0.01) and consumed more energy (food) from Day 56 onwards, but food efficiency was unaffected.
36701335	10	66	theme	fasting	1657:1663	arg1	triglycerides					1671:1683	fasting serum triglycerides	1657:1683	fasting serum triglycerides	1657:1683	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	4	67	theme	female	737:742	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	68	theme	different	572:580	arg1	levels					582:587	different levels	572:587	different levels of dietary Cr	572:601	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	0	69	theme	adult	191:195	arg1	age					197:199	young adult age	185:199	young adult age	185:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	5	70	dep	high	1030:1033	arg1	HCr					1036:1038	HCr	1036:1038	HCr	1036:1038	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	70	dep	high	1030:1033	arg1	mg/kg					1053:1057	9.15 ± 0.65 mg/kg	1041:1057	9.15 ± 0.65 mg/kg	1041:1057	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	6	71	theme	ad	1085:1086	arg1	libitum					1088:1094	ad libitum	1085:1094	ad libitum	1085:1094	Diets were fed ad libitum for 12 weeks (83 days).
36701335	4	72	from	effects	561:567	arg1	weight					611:616	body weight	606:616	body weight	606:616	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	72	from	effects	561:567	arg1	composition					624:634	body composition	619:634	body composition	619:634	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	72	from	effects	561:567	arg1	intake					644:649	energy intake	637:649	energy intake	637:649	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	72	from	effects	561:567	arg1	efficiency					657:666	food efficiency	652:666	food efficiency	652:666	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	72	from	effects	561:567	arg1	parameters					682:691	metabolic parameters	672:691	metabolic parameters	672:691	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	5	73	theme	age	930:932	arg1	days					922:925	35 days	919:925	35 days of age	919:932	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	9	74	theme	oral	1368:1371	arg1	challenge					1381:1389	an oral glucose challenge	1365:1389	an oral glucose challenge (Day 77)	1365:1398	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	9	74	theme	oral	1368:1371	arg1	Day					1392:1394	Day 77	1392:1397	Day 77	1392:1397	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	0	75	from	intake	47:52	arg1	rats					115:118	male and female rats	99:118	male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age	99:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	9	76	theme	chromium	1409:1416	arg1	levels					1418:1423	dietary chromium levels	1401:1423	dietary chromium levels	1401:1423	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	0	77	dep	high-fat	137:144	arg1	high-sucrose					147:158	high-sucrose	147:158	high-sucrose	147:158	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	4	78	dep	diet	851:854	arg1	high-sucrose					838:849	a moderately high-fat, high-sucrose diet	815:854	high-sucrose	838:849	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	9	79	theme	NCr	1570:1572	arg1	group					1574:1578	the NCr group	1566:1578	the NCr group	1566:1578	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	5	80	dep	rats	875:878	arg1	n					881:881	n	881:881	n = 10 males and 10 females/group	881:913	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	81	theme	9.15	1041:1044	arg1	HCr					1036:1038	HCr	1036:1038	HCr	1036:1038	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	81	theme	9.15	1041:1044	arg1	mg/kg					1053:1057	9.15 ± 0.65 mg/kg	1041:1057	9.15 ± 0.65 mg/kg	1041:1057	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	0	82	theme	high-fat	137:144	arg1	diet					160:163	a moderately high-fat, high-sucrose diet	124:163	a moderately high-fat, high-sucrose diet from peripuberty to young adult age	124:199	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	8	83	dep	weighed	1244:1250	arg1	p<0.01					1258:1263	p<0.01	1258:1263	p<0.01	1258:1263	Compared to the NCr group, the LCr group weighed more (p<0.01) and consumed more energy (food) from Day 56 onwards, but food efficiency was unaffected.
36701335	4	84	from	metabolism	714:723	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	10	85	theme	study	1599:1603	arg1	end					1588:1590	the end	1584:1590	the end of the study	1584:1603	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	5	86	dep	normal	995:1000	arg1	mg/kg					1020:1024	1.20 ± 0.11 mg/kg	1008:1024	1.20 ± 0.11 mg/kg	1008:1024	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	86	dep	normal	995:1000	arg1	NCr					1003:1005	NCr	1003:1005	NCr	1003:1005	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	4	87	theme	Cr	600:601	arg1	levels					582:587	different levels	572:587	different levels of dietary Cr	572:601	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	5	88	theme	high	1030:1033	arg1	diets					1063:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets	961:1067	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	11	89	theme	HCr	1800:1802	arg1	group					1804:1808	the HCr group	1796:1808	the HCr group	1796:1808	Effects were similar in both sexes and not observed in the HCr group.
36701335	0	90	theme	circulating	58:68	arg1	triglycerides					70:82	circulating triglycerides	58:82	circulating triglycerides	58:82	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	9	91	theme	dietary	1401:1407	arg1	levels					1418:1423	dietary chromium levels	1401:1423	dietary chromium levels	1401:1423	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	12	92	theme	dietary	1836:1842	arg1	Cr					1844:1845	low dietary Cr	1832:1845	low dietary Cr	1832:1845	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	4	93	theme	body	606:609	arg1	weight					611:616	body weight	606:616	body weight	606:616	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	94	theme	high-fat	828:835	arg1	diet					851:854	a moderately high-fat, high-sucrose diet	815:854	diet	851:854	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	10	95	theme	whole-body	1606:1615	arg1	fat					1617:1619	whole-body fat	1606:1619	whole-body fat	1606:1619	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	1	96	theme	intakes	304:310	arg1	effects					284:290	the effects	280:290	the effects of chromium intakes on metabolic parameters	280:334	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	1	96	theme	intakes	304:310	arg1	unclear					340:346	unclear	340:346	unclear	340:346	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	4	97	from	efficiency	657:666	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	5	98	theme	CD	872:873	arg1	rats					875:878	Sprague-Dawley CD rats	857:878	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age	857:932	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	2	99	theme	low	445:447	arg1	quantities					449:458	feeding low quantities	437:458	feeding low quantities	437:458	Cr is listed as a potentially beneficial element for rodents based on studies that show feeding low quantities affect glucose metabolism.
36701335	4	100	theme	metabolic	672:680	arg1	parameters					682:691	metabolic parameters	672:691	metabolic parameters	672:691	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	0	101	theme	low	2:4	arg1	diet					15:18	A low chromium diet	0:18	A low chromium diet	0:18	A low chromium diet increases body fat, energy intake and circulating triglycerides and insulin in male and female rats fed a moderately high-fat, high-sucrose diet from peripuberty to young adult age.
36701335	1	102	theme	metabolic	315:323	arg1	parameters					325:334	metabolic parameters	315:334	metabolic parameters	315:334	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	8	103	theme	NCr	1219:1221	arg1	group					1223:1227	the NCr group	1215:1227	the NCr group	1215:1227	Compared to the NCr group, the LCr group weighed more (p<0.01) and consumed more energy (food) from Day 56 onwards, but food efficiency was unaffected.
36701335	2	104	theme	beneficial	379:388	arg1	element					390:396	a potentially beneficial element	365:396	a potentially beneficial element for rodents based on studies that show feeding low quantities affect glucose metabolism	365:484	Cr is listed as a potentially beneficial element for rodents based on studies that show feeding low quantities affect glucose metabolism.
36701335	2	104	theme	beneficial	379:388	arg1	Cr					349:350	Cr	349:350	Cr	349:350	Cr is listed as a potentially beneficial element for rodents based on studies that show feeding low quantities affect glucose metabolism.
36701335	4	105	theme	energy	637:642	arg1	intake					644:649	energy intake	637:649	energy intake	637:649	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	5	106	dep	low	965:967	arg1	mg/kg					987:991	0.33 ± 0.06 mg/kg	975:991	0.33 ± 0.06 mg/kg	975:991	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	5	106	dep	low	965:967	arg1	LCr					970:972	LCr	970:972	LCr	970:972	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	1	107	from	effects	284:290	arg1	parameters					325:334	metabolic parameters	315:334	metabolic parameters	315:334	Trivalent chromium (Cr) may function to potentiate the action of insulin, but the effects of chromium intakes on metabolic parameters are unclear.
36701335	9	108	theme	fasting	1460:1466	arg1	insulin					1475:1481	fasting plasma insulin	1460:1481	fasting plasma insulin	1460:1481	Following an oral glucose challenge (Day 77), dietary chromium levels did not affect plasma glucose, but fasting plasma insulin and insulin at 30 and 60 min after dosing were higher in the LCr group compared to the NCr group.
36701335	12	109	theme	nutritional	1959:1969	arg1	effects					1994:2000	the nutritional and/or pharmacological effects	1955:2000	the nutritional and/or pharmacological effects of Cr	1955:2006	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	4	110	theme	metabolism	714:723	arg1	weight					611:616	body weight	606:616	body weight	606:616	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	110	theme	metabolism	714:723	arg1	composition					624:634	body composition	619:634	body composition	619:634	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	110	theme	metabolism	714:723	arg1	intake					644:649	energy intake	637:649	energy intake	637:649	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	110	theme	metabolism	714:723	arg1	efficiency					657:666	food efficiency	652:666	food efficiency	652:666	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	4	110	theme	metabolism	714:723	arg1	parameters					682:691	metabolic parameters	672:691	metabolic parameters	672:691	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	7	111	dep	similar	1167:1173	arg1	p≥0.05					1176:1181	p≥0.05	1176:1181	p≥0.05	1176:1181	At baseline, body weights and composition were similar (p≥0.05) among diet groups.
36701335	12	112	theme	pharmacological	1978:1992	arg1	effects					1994:2000	the nutritional and/or pharmacological effects	1955:2000	the nutritional and/or pharmacological effects of Cr	1955:2006	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	4	113	theme	male	728:731	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
36701335	8	114	theme	more	1279:1282	arg1	energy					1284:1289	more energy	1279:1289	more energy (food)	1279:1296	Compared to the NCr group, the LCr group weighed more (p<0.01) and consumed more energy (food) from Day 56 onwards, but food efficiency was unaffected.
36701335	8	114	theme	more	1279:1282	arg1	food					1292:1295	food	1292:1295	food	1292:1295	Compared to the NCr group, the LCr group weighed more (p<0.01) and consumed more energy (food) from Day 56 onwards, but food efficiency was unaffected.
36701335	5	115	theme	=	883:883	arg1	n					881:881	n	881:881	n = 10 males and 10 females/group	881:913	Sprague-Dawley CD rats (n = 10 males and 10 females/group) at 35 days of age were assigned by weight to the low (LCr, 0.33 ± 0.06 mg/kg), normal (NCr, 1.20 ± 0.11 mg/kg) or high (HCr, 9.15 ± 0.65 mg/kg) Cr diets.
36701335	10	116	theme	body	1630:1633	arg1	fat					1635:1637	accrued body fat	1622:1637	accrued body fat from baseline	1622:1651	At the end of the study, whole-body fat, accrued body fat from baseline and fasting serum triglycerides were higher in the LCr group compared to the NCr group.
36701335	12	117	from	diseases	1894:1901	arg1	common					1876:1881	common	1876:1881	common	1876:1881	These data show that low dietary Cr affects metabolic parameters common in chronic diseases underscoring the need for clinical trials to define the nutritional and/or pharmacological effects of Cr.
36701335	4	118	from	weight	611:616	arg1	rats					744:747	male and female rats	728:747	male and female rats	728:747	This study examined the effects of different levels of dietary Cr on body weight, body composition, energy intake, food efficiency and metabolic parameters of lipid and glucose metabolism in male and female rats when fed from peripuberty to young adult age in the background of a moderately high-fat, high-sucrose diet.
35858469	7	0	theme	inflammation	1456:1467	arg1	features					1444:1451	the macroscopic and microscopic features	1412:1451	the macroscopic and microscopic features of inflammation	1412:1467	Results showed that MSC-Ex mitigated colitis by significantly relieving the macroscopic and microscopic features of inflammation, modulating the gut metagenomics and metabolomics profile, and increasing colonic FXR.
35858469	3	1	theme	regulatory	681:690	arg1	effect					692:697	the regulatory effect	677:697	the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines	677:869	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	6	2	theme	fecal	1207:1211	arg1	samples					1213:1219	fecal samples	1207:1219	fecal samples	1207:1219	Mice fecal samples were obtained for metagenomics and metabolomics analysis via 16S rRNA gene sequencing and UHPLC/Q-TOF-MS respectively.
35858469	6	3	dep	Mice	1202:1205	arg1	obtained					1226:1233	obtained	1226:1233	were obtained for metagenomics and metabolomics analysis via 16S rRNA gene sequencing and UHPLC/Q-TOF-MS respectively	1221:1337	Mice fecal samples were obtained for metagenomics and metabolomics analysis via 16S rRNA gene sequencing and UHPLC/Q-TOF-MS respectively.
35858469	0	4	link	cell-derived	17:28	arg1	exosome					30:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	3	5	theme	gut	716:718	arg1	diversity					745:753	diversity	745:753	diversity	745:753	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	5	theme	gut	716:718	arg1	composition					729:739	composition	729:739	composition	729:739	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	5	theme	gut	716:718	arg1	bacteria					720:727	the gut bacteria composition and diversity	712:753	the gut bacteria composition and diversity	712:753	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	4	6	dep	groups	1004:1009	arg1	DSS					1021:1023	DSS	1021:1023	DSS	1021:1023	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	4	6	dep	groups	1004:1009	arg1	groups					1004:1009	three groups	998:1009	three groups: control, DSS, and MSC-Ex groups	998:1042	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	4	6	dep	groups	1004:1009	arg1	control					1012:1018	control	1012:1018	control	1012:1018	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	4	6	dep	groups	1004:1009	arg1	groups					1037:1042	MSC-Ex groups	1030:1042	MSC-Ex groups	1030:1042	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	3	7	theme	therapeutic	579:589	arg1	effect					591:596	the promising therapeutic effect	565:596	the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD	565:654	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	1	8	theme	gut	199:201	arg1	dysregulation					210:222	chronic gut immune dysregulation	191:222	chronic gut immune dysregulation	191:222	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	8	9	dep	improved	1563:1570	arg1	decreasing					1746:1755	decreasing	1746:1755	decreasing disease-associated bacteria	1746:1783	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	8	9	dep	improved	1563:1570	arg1	enhancing					1824:1832	enhancing	1824:1832	enhancing other vital cellular functions	1824:1863	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	8	9	dep	improved	1563:1570	arg1	decreasing					1786:1795	decreasing	1786:1795	decreasing detrimental functions	1786:1817	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	8	9	dep	improved	1563:1570	arg1	increasing					1698:1707	increasing	1698:1707	increasing the abundance of 'healthy' bacteria	1698:1743	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	3	10	from	effect	692:697	arg1	metabolites					756:766	metabolites	756:766	metabolites	756:766	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	10	from	effect	692:697	arg1	bacteria					720:727	the gut bacteria composition and diversity	712:753	the gut bacteria composition and diversity	712:753	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	10	from	effect	692:697	arg1	composition					729:739	composition	729:739	composition	729:739	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	10	from	effect	692:697	arg1	cytokines					861:869	key inflammatory and anti-inflammatory cytokines	822:869	key inflammatory and anti-inflammatory cytokines	822:869	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	10	from	effect	692:697	arg1	diversity					745:753	diversity	745:753	diversity	745:753	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	10	from	effect	692:697	arg1	functions					787:795	their related functions	773:795	their related functions	773:795	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	11	theme	stem	613:616	arg1	exosomes					631:638	mesenchymal stem cell-derived exosomes	601:638	mesenchymal stem cell-derived exosomes (MSC-Ex)	601:647	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	11	theme	stem	613:616	arg1	MSC-Ex					641:646	MSC-Ex	641:646	MSC-Ex	641:646	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	1	12	theme	altered	228:234	arg1	composition					262:272	altered microbiome and metabolite composition	228:272	altered microbiome and metabolite composition	228:272	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	9	13	theme	gut	1957:1959	arg1	axis					1991:1994	the gut metagenomics-metabolomics-FXR axis	1953:1994	the gut metagenomics-metabolomics-FXR axis	1953:1994	For the first time, we demonstrate that MSC-Ex mitigates colitis in mice by modulating the gut metagenomics-metabolomics-FXR axis, thus providing potential therapeutic targets.
35858469	8	14	theme	disease-associated	1757:1774	arg1	bacteria					1776:1783	disease-associated bacteria	1757:1783	disease-associated bacteria	1757:1783	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	1	15	theme	Inflammatory	139:150	arg1	IBD					167:169	IBD	167:169	IBD	167:169	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	1	15	theme	Inflammatory	139:150	arg1	disease					158:164	Inflammatory bowel disease	139:164	Inflammatory bowel disease (IBD)	139:170	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	3	16	theme	key	822:824	arg1	cytokines					861:869	key inflammatory and anti-inflammatory cytokines	822:869	key inflammatory and anti-inflammatory cytokines	822:869	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	2	17	theme	acid	493:496	arg1	pool					498:501	the bile acid pool	484:501	the bile acid pool	484:501	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	3	18	theme	related	779:785	arg1	functions					787:795	their related functions	773:795	their related functions	773:795	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	19	theme	exosomes	631:638	arg1	effect					591:596	the promising therapeutic effect	565:596	the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD	565:654	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	8	20	theme	detrimental	1797:1807	arg1	functions					1809:1817	detrimental functions	1797:1817	detrimental functions	1797:1817	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	4	21	theme	mice	962:965	arg1	model					946:950	The dextran sulfate sodium (DSS)-induced IBD model	901:950	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice	901:965	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	9	22	theme	potential	2012:2020	arg1	targets					2034:2040	potential therapeutic targets	2012:2040	potential therapeutic targets	2012:2040	For the first time, we demonstrate that MSC-Ex mitigates colitis in mice by modulating the gut metagenomics-metabolomics-FXR axis, thus providing potential therapeutic targets.
35858469	7	23	theme	colonic	1543:1549	arg1	FXR					1551:1553	colonic FXR	1543:1553	colonic FXR	1543:1553	Results showed that MSC-Ex mitigated colitis by significantly relieving the macroscopic and microscopic features of inflammation, modulating the gut metagenomics and metabolomics profile, and increasing colonic FXR.
35858469	6	24	theme	gene	1291:1294	arg1	sequencing					1296:1305	rRNA gene sequencing	1286:1305	rRNA gene sequencing	1286:1305	Mice fecal samples were obtained for metagenomics and metabolomics analysis via 16S rRNA gene sequencing and UHPLC/Q-TOF-MS respectively.
35858469	4	25	theme	IBD	942:944	arg1	model					946:950	The dextran sulfate sodium (DSS)-induced IBD model	901:950	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice	901:965	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	0	26	theme	receptor	124:131	arg1	axis					133:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	8	27	theme	vital	1840:1844	arg1	functions					1855:1863	other vital cellular functions	1834:1863	other vital cellular functions	1834:1863	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	3	28	link	cell-derived	618:629	arg1	exosomes					631:638	mesenchymal stem cell-derived exosomes	601:638	mesenchymal stem cell-derived exosomes (MSC-Ex)	601:647	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	28	link	cell-derived	618:629	arg1	MSC-Ex					641:646	MSC-Ex	641:646	MSC-Ex	641:646	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	2	29	theme	chemical	377:384	arg1	communications					386:399	the chemical communications	373:399	the chemical communications between the intestinal microbiota and the host immune system	373:460	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	0	30	theme	Mesenchymal	0:10	arg1	exosome					30:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	5	31	theme	eosin	1121:1125	arg1	staining					1133:1140	hematoxylin and eosin (H&E) staining	1105:1140	hematoxylin and eosin (H&E) staining	1105:1140	Post administration of MSC-Ex, the effect was evaluated via hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC), qRT-PCR, and western blotting.
35858469	2	32	theme	host	443:446	arg1	system					455:460	the host immune system	439:460	the host immune system	439:460	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	2	33	theme	Bile	275:278	arg1	acids					280:284	Bile acids	275:284	Bile acids	275:284	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	2	33	theme	Bile	275:278	arg1	receptor					330:337	the farnesoid X receptor	314:337	the farnesoid X receptor (FXR)	314:343	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	0	34	theme	cell-derived	17:28	arg1	exosome					30:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	7	35	theme	metabolomics	1506:1517	arg1	profile					1519:1525	metabolomics profile	1506:1525	metabolomics profile	1506:1525	Results showed that MSC-Ex mitigated colitis by significantly relieving the macroscopic and microscopic features of inflammation, modulating the gut metagenomics and metabolomics profile, and increasing colonic FXR.
35858469	2	36	theme	intestinal	413:422	arg1	microbiota					424:433	the intestinal microbiota	409:433	the intestinal microbiota	409:433	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	5	37	theme	hematoxylin	1105:1115	arg1	staining					1133:1140	hematoxylin and eosin (H&E) staining	1105:1140	hematoxylin and eosin (H&E) staining	1105:1140	Post administration of MSC-Ex, the effect was evaluated via hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC), qRT-PCR, and western blotting.
35858469	4	38	theme	MSC-Ex	1030:1035	arg1	groups					1037:1042	MSC-Ex groups	1030:1042	MSC-Ex groups	1030:1042	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	1	39	theme	microbiome	236:245	arg1	composition					262:272	altered microbiome and metabolite composition	228:272	altered microbiome and metabolite composition	228:272	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	8	40	theme	OTUs	1647:1650	arg1	structure					1634:1642	the structure	1630:1642	the structure of OTUs and colitis-induced reduction	1630:1680	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	1	41	theme	metabolite	251:260	arg1	composition					262:272	altered microbiome and metabolite composition	228:272	altered microbiome and metabolite composition	228:272	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	8	42	theme	microbiota	1580:1589	arg1	composition					1591:1601	the gut microbiota composition	1572:1601	the gut microbiota composition	1572:1601	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	3	43	theme	MSC-Ex	702:707	arg1	effect					692:697	the regulatory effect	677:697	the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines	677:869	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	2	44	theme	crucial	352:358	arg1	component					360:368	a crucial component	350:368	a crucial component of the chemical communications between the intestinal microbiota and the host immune system	350:460	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	8	45	theme	colitis-induced	1656:1670	arg1	reduction					1672:1680	colitis-induced reduction	1656:1680	colitis-induced reduction	1656:1680	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	7	46	theme	microscopic	1432:1442	arg1	features					1444:1451	the macroscopic and microscopic features	1412:1451	the macroscopic and microscopic features of inflammation	1412:1467	Results showed that MSC-Ex mitigated colitis by significantly relieving the macroscopic and microscopic features of inflammation, modulating the gut metagenomics and metabolomics profile, and increasing colonic FXR.
35858469	7	47	theme	macroscopic	1416:1426	arg1	features					1444:1451	the macroscopic and microscopic features	1412:1451	the macroscopic and microscopic features of inflammation	1412:1467	Results showed that MSC-Ex mitigated colitis by significantly relieving the macroscopic and microscopic features of inflammation, modulating the gut metagenomics and metabolomics profile, and increasing colonic FXR.
35858469	3	48	theme	promising	569:577	arg1	effect					591:596	the promising therapeutic effect	565:596	the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD	565:654	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	1	49	theme	chronic	191:197	arg1	dysregulation					210:222	chronic gut immune dysregulation	191:222	chronic gut immune dysregulation	191:222	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	2	50	theme	farnesoid	318:326	arg1	FXR					340:342	FXR	340:342	FXR	340:342	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	2	50	theme	farnesoid	318:326	arg1	receptor					330:337	the farnesoid X receptor	314:337	the farnesoid X receptor (FXR)	314:343	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	1	51	theme	immune	203:208	arg1	dysregulation					210:222	chronic gut immune dysregulation	191:222	chronic gut immune dysregulation	191:222	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	2	52	theme	X	328:328	arg1	FXR					340:342	FXR	340:342	FXR	340:342	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	2	52	theme	X	328:328	arg1	receptor					330:337	the farnesoid X receptor	314:337	the farnesoid X receptor (FXR)	314:343	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	6	53	theme	metabolomics	1256:1267	arg1	analysis					1269:1276	metabolomics analysis	1256:1276	metabolomics analysis	1256:1276	Mice fecal samples were obtained for metagenomics and metabolomics analysis via 16S rRNA gene sequencing and UHPLC/Q-TOF-MS respectively.
35858469	3	54	theme	mesenchymal	601:611	arg1	exosomes					631:638	mesenchymal stem cell-derived exosomes	601:638	mesenchymal stem cell-derived exosomes (MSC-Ex)	601:647	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	54	theme	mesenchymal	601:611	arg1	MSC-Ex					641:646	MSC-Ex	641:646	MSC-Ex	641:646	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	8	55	theme	healthy	1727:1733	arg1	bacteria					1736:1743	'healthy' bacteria	1726:1743	'healthy' bacteria	1726:1743	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	8	56	theme	bacteria	1736:1743	arg1	abundance					1713:1721	the abundance	1709:1721	the abundance of 'healthy' bacteria	1709:1743	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	9	57	theme	metagenomics-metabolomics-FXR	1961:1989	arg1	axis					1991:1994	the gut metagenomics-metabolomics-FXR axis	1953:1994	the gut metagenomics-metabolomics-FXR axis	1953:1994	For the first time, we demonstrate that MSC-Ex mitigates colitis in mice by modulating the gut metagenomics-metabolomics-FXR axis, thus providing potential therapeutic targets.
35858469	1	58	theme	bowel	152:156	arg1	IBD					167:169	IBD	167:169	IBD	167:169	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	1	58	theme	bowel	152:156	arg1	disease					158:164	Inflammatory bowel disease	139:164	Inflammatory bowel disease (IBD)	139:170	Inflammatory bowel disease (IBD) is associated with chronic gut immune dysregulation and altered microbiome and metabolite composition.
35858469	3	59	theme	anti-inflammatory	843:859	arg1	cytokines					861:869	key inflammatory and anti-inflammatory cytokines	822:869	key inflammatory and anti-inflammatory cytokines	822:869	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	9	60	from	colitis	1923:1929	arg1	mice					1934:1937	mice	1934:1937	mice	1934:1937	For the first time, we demonstrate that MSC-Ex mitigates colitis in mice by modulating the gut metagenomics-metabolomics-FXR axis, thus providing potential therapeutic targets.
35858469	3	61	theme	cell-derived	618:629	arg1	exosomes					631:638	mesenchymal stem cell-derived exosomes	601:638	mesenchymal stem cell-derived exosomes (MSC-Ex)	601:647	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	61	theme	cell-derived	618:629	arg1	MSC-Ex					641:646	MSC-Ex	641:646	MSC-Ex	641:646	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	62	theme	inflammatory	826:837	arg1	cytokines					861:869	key inflammatory and anti-inflammatory cytokines	822:869	key inflammatory and anti-inflammatory cytokines	822:869	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	2	63	theme	bile	488:491	arg1	pool					498:501	the bile acid pool	484:501	the bile acid pool	484:501	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	0	64	theme	metagenomics-metabolomics-farnesoid	86:120	arg1	axis					133:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	0	65	theme	gut	82:84	arg1	axis					133:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	4	66	theme	BABL/C	955:960	arg1	mice					962:965	BABL/C mice	955:965	BABL/C mice	955:965	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	9	67	theme	therapeutic	2022:2032	arg1	targets					2034:2040	potential therapeutic targets	2012:2040	potential therapeutic targets	2012:2040	For the first time, we demonstrate that MSC-Ex mitigates colitis in mice by modulating the gut metagenomics-metabolomics-FXR axis, thus providing potential therapeutic targets.
35858469	5	68	theme	western	1184:1190	arg1	blotting					1192:1199	western blotting	1184:1199	western blotting	1184:1199	Post administration of MSC-Ex, the effect was evaluated via hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC), qRT-PCR, and western blotting.
35858469	0	69	theme	X	122:122	arg1	axis					133:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	the gut metagenomics-metabolomics-farnesoid X receptor axis	78:136	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	2	70	theme	intestinal	510:519	arg1	homeostasis					521:531	intestinal homeostasis	510:531	intestinal homeostasis	510:531	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	6	71	theme	rRNA	1286:1289	arg1	sequencing					1296:1305	rRNA gene sequencing	1286:1305	rRNA gene sequencing	1286:1305	Mice fecal samples were obtained for metagenomics and metabolomics analysis via 16S rRNA gene sequencing and UHPLC/Q-TOF-MS respectively.
35858469	4	72	theme	-induced	933:940	arg1	model					946:950	The dextran sulfate sodium (DSS)-induced IBD model	901:950	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice	901:965	The dextran sulfate sodium (DSS)-induced IBD model of BABL/C mice was established, consisting of three groups: control, DSS, and MSC-Ex groups.
35858469	2	73	from	alterations	469:479	arg1	pool					498:501	the bile acid pool	484:501	the bile acid pool	484:501	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	0	74	theme	axis	133:136	arg1	modulation					64:73	the modulation	60:73	the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis	60:136	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	0	75	theme	stem	12:15	arg1	exosome					30:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome	0:36	Mesenchymal stem cell-derived exosome mitigates colitis via the modulation of the gut metagenomics-metabolomics-farnesoid X receptor axis.
35858469	5	76	theme	MSC-Ex	1068:1073	arg1	administration					1050:1063	administration	1050:1063	administration of MSC-Ex	1050:1073	Post administration of MSC-Ex, the effect was evaluated via hematoxylin and eosin (H&E) staining, immunohistochemistry (IHC), qRT-PCR, and western blotting.
35858469	8	77	theme	other	1834:1838	arg1	functions					1855:1863	other vital cellular functions	1834:1863	other vital cellular functions	1834:1863	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	3	78	theme	IBD	896:898	arg1	mitigation					882:891	the mitigation	878:891	the mitigation of IBD	878:898	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	2	79	theme	immune	448:453	arg1	system					455:460	the host immune system	439:460	the host immune system	439:460	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	3	80	from	effect	591:596	arg1	IBD					652:654	IBD	652:654	IBD	652:654	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	8	81	theme	cellular	1846:1853	arg1	functions					1855:1863	other vital cellular functions	1834:1863	other vital cellular functions	1834:1863	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	9	82	theme	first	1874:1878	arg1	time					1880:1883	the first time	1870:1883	the first time	1870:1883	For the first time, we demonstrate that MSC-Ex mitigates colitis in mice by modulating the gut metagenomics-metabolomics-FXR axis, thus providing potential therapeutic targets.
35858469	3	83	dep	bacteria	720:727	arg1	diversity					745:753	diversity	745:753	diversity	745:753	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	83	dep	bacteria	720:727	arg1	composition					729:739	composition	729:739	composition	729:739	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	3	83	dep	bacteria	720:727	arg1	bacteria					720:727	the gut bacteria composition and diversity	712:753	the gut bacteria composition and diversity	712:753	Considering the promising therapeutic effect of mesenchymal stem cell-derived exosomes (MSC-Ex) on IBD, this study assessed the regulatory effect of MSC-Ex on the gut bacteria composition and diversity, metabolites, and their related functions and pathways, as well as key inflammatory and anti-inflammatory cytokines during the mitigation of IBD.
35858469	7	84	theme	gut	1485:1487	arg1	metagenomics					1489:1500	the gut metagenomics	1481:1500	the gut metagenomics	1481:1500	Results showed that MSC-Ex mitigated colitis by significantly relieving the macroscopic and microscopic features of inflammation, modulating the gut metagenomics and metabolomics profile, and increasing colonic FXR.
35858469	8	85	theme	gut	1576:1578	arg1	composition					1591:1601	the gut microbiota composition	1572:1601	the gut microbiota composition	1572:1601	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
35858469	2	86	theme	communications	386:399	arg1	component					360:368	a crucial component	350:368	a crucial component of the chemical communications between the intestinal microbiota and the host immune system	350:460	Bile acids and their receptors such as the farnesoid X receptor (FXR) form a crucial component of the chemical communications between the intestinal microbiota and the host immune system; thus, alterations in the bile acid pool affect intestinal homeostasis and exacerbate IBD.
35858469	8	87	theme	reduction	1672:1680	arg1	structure					1634:1642	the structure	1630:1642	the structure of OTUs and colitis-induced reduction	1630:1680	MSC-Ex improved the gut microbiota composition by significantly restoring the structure of OTUs and colitis-induced reduction in α-diversity, increasing the abundance of 'healthy' bacteria, decreasing disease-associated bacteria, decreasing detrimental functions, and enhancing other vital cellular functions.
36972711	7	0	theme	delivery	1560:1567	arg1	CS-PA/CNP					1376:1384	CS-PA/CNP	1376:1384	CS-PA/CNP	1376:1384	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	7	0	theme	delivery	1560:1567	arg1	platform					1569:1576	a drug delivery platform	1553:1576	a drug delivery platform	1553:1576	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	4	1	from	embedded	702:709	arg1	hydrogel					718:725	the hydrogel	714:725	the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities	714:838	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	0	2	theme	Hydrogel	98:105	arg1	System					107:112	A Nanoparticle-embedded Functional Hydrogel System	63:112	A Nanoparticle-embedded Functional Hydrogel System	63:112	Alleviate Periodontitis and its Comorbidity Hypertension using A Nanoparticle-embedded Functional Hydrogel System.
36972711	6	3	theme	immunoregulatory	1134:1149	arg1	effects					1151:1157	excellent immunoregulatory effects	1124:1157	excellent immunoregulatory effects	1124:1157	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	4	4	theme	anti-inflammatory	811:827	arg1	activities					829:838	long-term anti-inflammatory activities	801:838	long-term anti-inflammatory activities	801:838	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	6	5	theme	metabolism	1341:1350	arg1	pathway					1352:1358	the glutathione metabolism pathway	1325:1358	the glutathione metabolism pathway	1325:1358	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	5	6	theme	gingival	931:938	arg1	sulcus					940:945	gingival sulcus	931:945	gingival sulcus	931:945	In a mouse model of periodontitis complicated with hypertension, CS-PA/CNP was applied to gingival sulcus and produced an optimal therapeutic effect on periodontitis and hypertension simultaneously.
36972711	3	7	dep	-modified	545:553	arg1	peptide					532:538	antimicrobial peptide	518:538	antimicrobial peptide	518:538	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	7	8	with	periodontitis	1627:1639	arg1	pathogenesis					1658:1669	complicated pathogenesis	1646:1669	complicated pathogenesis	1646:1669	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	7	9	theme	therapeutic	1603:1613	arg1	options					1615:1621	combinatorial therapeutic options	1589:1621	combinatorial therapeutic options for periodontitis with complicated pathogenesis	1589:1669	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	6	10	theme	anti-inflammatory	1275:1291	arg1	capacity					1293:1300	thus the anti-inflammatory capacity	1266:1300	thus the anti-inflammatory capacity of macrophages	1266:1315	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	6	11	theme	myeloid	1210:1216	arg1	cells					1218:1222	myeloid cells	1210:1222	myeloid cells	1210:1222	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	7	12	theme	potential	1440:1448	arg1	value					1473:1477	potential clinical translational value	1440:1477	potential clinical translational value	1440:1477	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	2	13	theme	hydrogel	285:292	arg1	approach					294:301	a controlled-release composite hydrogel approach	254:301	a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities	254:358	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	2	13	theme	hydrogel	285:292	arg1	resolution					365:374	a resolution	363:374	a resolution to achieve the goal of co-treatment of comorbidities	363:427	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	7	14	theme	clinical	1450:1457	arg1	value					1473:1477	potential clinical translational value	1440:1477	potential clinical translational value	1440:1477	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	2	15	theme	comorbidities	415:427	arg1	co-treatment					399:410	co-treatment	399:410	co-treatment of comorbidities	399:427	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	6	16	theme	therapeutic	1044:1054	arg1	mechanisms					1056:1065	The therapeutic mechanisms	1040:1065	The therapeutic mechanisms	1040:1065	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	6	17	theme	cells	1218:1222	arg1	accumulation					1178:1189	the accumulation	1174:1189	the accumulation of lymphocytes and myeloid cells	1174:1222	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	6	18	theme	antioxidant	1241:1251	arg1	capacity					1253:1260	the antioxidant capacity	1237:1260	the antioxidant capacity	1237:1260	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	2	19	theme	composite	275:283	arg1	approach					294:301	a controlled-release composite hydrogel approach	254:301	a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities	254:358	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	2	19	theme	composite	275:283	arg1	resolution					365:374	a resolution	363:374	a resolution to achieve the goal of co-treatment of comorbidities	363:427	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	4	20	theme	biodegradable	663:675	arg1	nanoparticles					677:689	biodegradable nanoparticles	663:689	biodegradable nanoparticles (CNP)	663:695	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	4	20	theme	biodegradable	663:675	arg1	CNP					692:694	CNP	692:694	CNP	692:694	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	7	21	theme	combinatorial	1589:1601	arg1	options					1615:1621	combinatorial therapeutic options	1589:1621	combinatorial therapeutic options for periodontitis with complicated pathogenesis	1589:1669	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	2	22	theme	anti-inflammatory	331:347	arg1	activities					349:358	dual antibacterial and anti-inflammatory activities	308:358	dual antibacterial and anti-inflammatory activities	308:358	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	2	23	theme	co-treatment	399:410	arg1	goal					391:394	the goal	387:394	the goal of co-treatment of comorbidities	387:427	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	0	24	theme	Alleviate	0:8	arg1	Periodontitis					10:22	Alleviate Periodontitis	0:22	Alleviate Periodontitis	0:22	Alleviate Periodontitis and its Comorbidity Hypertension using A Nanoparticle-embedded Functional Hydrogel System.
36972711	6	25	theme	macrophages	1305:1315	arg1	capacity					1253:1260	the antioxidant capacity	1237:1260	the antioxidant capacity	1237:1260	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	6	25	theme	macrophages	1305:1315	arg1	capacity					1293:1300	thus the anti-inflammatory capacity	1266:1300	thus the anti-inflammatory capacity of macrophages	1266:1315	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	7	26	theme	complicated	1646:1656	arg1	pathogenesis					1658:1669	complicated pathogenesis	1646:1669	complicated pathogenesis	1646:1669	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	4	27	theme	encapsulation	743:755	arg1	efficiency					757:766	high encapsulation efficiency	738:766	high encapsulation efficiency	738:766	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	7	28	theme	periodontitis	1502:1514	arg1	co-treatment					1486:1497	the co-treatment	1482:1497	the co-treatment of periodontitis and hypertension	1482:1531	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	7	29	theme	translational	1459:1471	arg1	value					1473:1477	potential clinical translational value	1440:1477	potential clinical translational value	1440:1477	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	5	30	theme	mouse	846:850	arg1	model					852:856	a mouse model	844:856	a mouse model of periodontitis complicated with hypertension	844:903	In a mouse model of periodontitis complicated with hypertension, CS-PA/CNP was applied to gingival sulcus and produced an optimal therapeutic effect on periodontitis and hypertension simultaneously.
36972711	6	31	theme	glutathione	1329:1339	arg1	pathway					1352:1358	the glutathione metabolism pathway	1325:1358	the glutathione metabolism pathway	1325:1358	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	2	32	theme	antibacterial	313:325	arg1	activities					349:358	dual antibacterial and anti-inflammatory activities	308:358	dual antibacterial and anti-inflammatory activities	308:358	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	1	33	theme	same	208:211	arg1	time					213:216	the same time	204:216	the same time	204:216	Periodontitis and hypertension often occur as comorbidities, which need to be treated at the same time.
36972711	3	34	theme	polyethylene	555:566	arg1	PEG					576:578	PEG	576:578	PEG	576:578	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	3	34	theme	polyethylene	555:566	arg1	glycol					568:573	antimicrobial peptide (AMP)-modified polyethylene glycol	518:573	antimicrobial peptide (AMP)-modified polyethylene glycol (PEG)	518:579	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	2	35	theme	dual	308:311	arg1	activities					349:358	dual antibacterial and anti-inflammatory activities	308:358	dual antibacterial and anti-inflammatory activities	308:358	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	6	36	theme	excellent	1124:1132	arg1	effects					1151:1157	excellent immunoregulatory effects	1124:1157	excellent immunoregulatory effects	1124:1157	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	3	37	theme	inherent	463:470	arg1	properties					486:495	inherent antibacterial properties	463:495	inherent antibacterial properties	463:495	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	7	38	theme	hypertension	1520:1531	arg1	co-treatment					1486:1497	the co-treatment	1482:1497	the co-treatment of periodontitis and hypertension	1482:1531	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	3	39	theme	antibacterial	472:484	arg1	properties					486:495	inherent antibacterial properties	463:495	inherent antibacterial properties	463:495	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	3	40	theme	dual	591:594	arg1	hydrogel					610:617	a dual antibacterial hydrogel	589:617	a dual antibacterial hydrogel (CS-PA)	589:625	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	3	40	theme	dual	591:594	arg1	CS-PA					620:624	CS-PA	620:624	CS-PA	620:624	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	3	41	theme	antimicrobial	518:530	arg1	peptide					532:538	antimicrobial peptide	518:538	antimicrobial peptide	518:538	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	7	42	dep	superior	1407:1414	arg1	therapeutic					1416:1426	therapeutic	1416:1426	therapeutic	1416:1426	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	3	43	theme	antibacterial	596:608	arg1	hydrogel					610:617	a dual antibacterial hydrogel	589:617	a dual antibacterial hydrogel (CS-PA)	589:625	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	3	43	theme	antibacterial	596:608	arg1	CS-PA					620:624	CS-PA	620:624	CS-PA	620:624	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	7	44	theme	superior	1407:1414	arg1	effects					1428:1434	its superior therapeutic effects	1403:1434	its superior therapeutic effects	1403:1434	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	2	45	theme	controlled-release	256:273	arg1	approach					294:301	a controlled-release composite hydrogel approach	254:301	a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities	254:358	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	2	45	theme	controlled-release	256:273	arg1	resolution					365:374	a resolution	363:374	a resolution to achieve the goal of co-treatment of comorbidities	363:427	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
36972711	7	46	theme	drug	1555:1558	arg1	CS-PA/CNP					1376:1384	CS-PA/CNP	1376:1384	CS-PA/CNP	1376:1384	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	7	46	theme	drug	1555:1558	arg1	platform					1569:1576	a drug delivery platform	1553:1576	a drug delivery platform	1553:1576	In conclusion, CS-PA/CNP has demonstrated its superior therapeutic effects and potential clinical translational value in the co-treatment of periodontitis and hypertension, and also serves as a drug delivery platform to provide combinatorial therapeutic options for periodontitis with complicated pathogenesis.
36972711	4	47	theme	high	738:741	arg1	efficiency					757:766	high encapsulation efficiency	738:766	high encapsulation efficiency	738:766	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	4	48	theme	sustained	772:780	arg1	release					782:788	sustained release	772:788	sustained release	772:788	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	3	49	with	chitosan	444:451	arg1	properties					486:495	inherent antibacterial properties	463:495	inherent antibacterial properties	463:495	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	4	50	theme	long-term	801:809	arg1	activities					829:838	long-term anti-inflammatory activities	801:838	long-term anti-inflammatory activities	801:838	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	5	51	theme	therapeutic	971:981	arg1	effect					983:988	an optimal therapeutic effect	960:988	an optimal therapeutic effect	960:988	In a mouse model of periodontitis complicated with hypertension, CS-PA/CNP was applied to gingival sulcus and produced an optimal therapeutic effect on periodontitis and hypertension simultaneously.
36972711	4	52	from	hydrogel	718:725	arg1	embedded					702:709	embedded	702:709	embedded	702:709	Subsequently, curcumin loaded into biodegradable nanoparticles (CNP) were embedded in the hydrogel exhibiting high encapsulation efficiency and sustained release to achieve long-term anti-inflammatory activities.
36972711	5	53	theme	optimal	963:969	arg1	effect					983:988	an optimal therapeutic effect	960:988	an optimal therapeutic effect	960:988	In a mouse model of periodontitis complicated with hypertension, CS-PA/CNP was applied to gingival sulcus and produced an optimal therapeutic effect on periodontitis and hypertension simultaneously.
36972711	0	54	theme	Functional	87:96	arg1	System					107:112	A Nanoparticle-embedded Functional Hydrogel System	63:112	A Nanoparticle-embedded Functional Hydrogel System	63:112	Alleviate Periodontitis and its Comorbidity Hypertension using A Nanoparticle-embedded Functional Hydrogel System.
36972711	6	55	theme	lymphocytes	1194:1204	arg1	accumulation					1178:1189	the accumulation	1174:1189	the accumulation of lymphocytes and myeloid cells	1174:1222	The therapeutic mechanisms were deeply studied and indicated that CS-PA/CNP exerted excellent immunoregulatory effects by suppressing the accumulation of lymphocytes and myeloid cells and enhanced the antioxidant capacity and thus the anti-inflammatory capacity of macrophages through the glutathione metabolism pathway.
36972711	0	56	dep	Periodontitis	10:22	arg1	Hypertension					44:55	Hypertension	44:55	Hypertension	44:55	Alleviate Periodontitis and its Comorbidity Hypertension using A Nanoparticle-embedded Functional Hydrogel System.
36972711	5	57	theme	periodontitis	861:873	arg1	model					852:856	a mouse model	844:856	a mouse model of periodontitis complicated with hypertension	844:903	In a mouse model of periodontitis complicated with hypertension, CS-PA/CNP was applied to gingival sulcus and produced an optimal therapeutic effect on periodontitis and hypertension simultaneously.
36972711	0	58	theme	Nanoparticle-embedded	65:85	arg1	System					107:112	A Nanoparticle-embedded Functional Hydrogel System	63:112	A Nanoparticle-embedded Functional Hydrogel System	63:112	Alleviate Periodontitis and its Comorbidity Hypertension using A Nanoparticle-embedded Functional Hydrogel System.
36972711	3	59	theme	-modified	545:553	arg1	PEG					576:578	PEG	576:578	PEG	576:578	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	3	59	theme	-modified	545:553	arg1	glycol					568:573	antimicrobial peptide (AMP)-modified polyethylene glycol	518:573	antimicrobial peptide (AMP)-modified polyethylene glycol (PEG)	518:579	Specifically, chitosan (CS) with inherent antibacterial properties was crosslinked with antimicrobial peptide (AMP)-modified polyethylene glycol (PEG) to form a dual antibacterial hydrogel (CS-PA).
36972711	2	60	with	approach	294:301	arg1	activities					349:358	dual antibacterial and anti-inflammatory activities	308:358	dual antibacterial and anti-inflammatory activities	308:358	To resolve this issue, we proposed a controlled-release composite hydrogel approach with dual antibacterial and anti-inflammatory activities as a resolution to achieve the goal of co-treatment of comorbidities.
35304202	0	0	theme	cellulose	90:98	arg1	paper					100:104	bacterial cellulose paper	80:104	bacterial cellulose paper	80:104	Efficient fabrication, antibacterial and catalytic performance of Ag-NiO loaded bacterial cellulose paper.
35304202	0	1	theme	bacterial	80:88	arg1	paper					100:104	bacterial cellulose paper	80:104	bacterial cellulose paper	80:104	Efficient fabrication, antibacterial and catalytic performance of Ag-NiO loaded bacterial cellulose paper.
35304202	5	2	theme	red	642:644	arg1	reduction					623:631	chemical reduction	614:631	chemical reduction of congo red, methyl orange and methylene blue	614:678	The catalytic activity of the BC-Ag/NiO was evaluated against chemical reduction of congo red, methyl orange and methylene blue.
35304202	2	3	theme	Ag/NiO	342:347	arg1	preparation					327:337	the preparation	323:337	the preparation of Ag/NiO	323:347	A two-step facile hydrothermal method was employed for the preparation of Ag/NiO, followed by impregnation into BC hydrogel sheets.
35304202	8	4	theme	nanocomposites	979:992	arg1	hydrogel					994:1001	The synthesized nanocomposites hydrogel	963:1001	The synthesized nanocomposites hydrogel	963:1001	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	8	5	theme	antibacterial	1027:1039	arg1	activity					1041:1048	its antibacterial activity	1023:1048	its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus)	1023:1167	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	5	6	theme	BC-Ag/NiO	582:590	arg1	activity					566:573	The catalytic activity	552:573	The catalytic activity of the BC-Ag/NiO	552:590	The catalytic activity of the BC-Ag/NiO was evaluated against chemical reduction of congo red, methyl orange and methylene blue.
35304202	2	7	theme	facile	279:284	arg1	method					299:304	A two-step facile hydrothermal method	268:304	A two-step facile hydrothermal method	268:304	A two-step facile hydrothermal method was employed for the preparation of Ag/NiO, followed by impregnation into BC hydrogel sheets.
35304202	2	8	theme	hydrothermal	286:297	arg1	method					299:304	A two-step facile hydrothermal method	268:304	A two-step facile hydrothermal method	268:304	A two-step facile hydrothermal method was employed for the preparation of Ag/NiO, followed by impregnation into BC hydrogel sheets.
35304202	6	9	theme	0.12989 min-1	791:803	arg1	values					750:755	kapp values	745:755	kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1	745:803	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	2	10	theme	two-step	270:277	arg1	method					299:304	A two-step facile hydrothermal method	268:304	A two-step facile hydrothermal method	268:304	A two-step facile hydrothermal method was employed for the preparation of Ag/NiO, followed by impregnation into BC hydrogel sheets.
35304202	3	11	theme	%	404:404	arg1	composition					413:423	A 20% Ag/NiO composition	400:423	A 20% Ag/NiO composition	400:423	A 20% Ag/NiO composition was revealed by dry weight analysis.
35304202	2	12	theme	hydrogel	383:390	arg1	sheets					392:397	BC hydrogel sheets	380:397	BC hydrogel sheets	380:397	A two-step facile hydrothermal method was employed for the preparation of Ag/NiO, followed by impregnation into BC hydrogel sheets.
35304202	5	13	theme	chemical	614:621	arg1	reduction					623:631	chemical reduction	614:631	chemical reduction of congo red, methyl orange and methylene blue	614:678	The catalytic activity of the BC-Ag/NiO was evaluated against chemical reduction of congo red, methyl orange and methylene blue.
35304202	6	14	theme	0.1323 min-1	774:785	arg1	values					750:755	kapp values	745:755	kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1	745:803	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	1	15	theme	hydrogel	168:175	arg1	sheets					177:182	bacterial cellulose (BC) hydrogel sheets	143:182	bacterial cellulose (BC) hydrogel sheets	143:182	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	5	16	theme	catalytic	556:564	arg1	activity					566:573	The catalytic activity	552:573	The catalytic activity of the BC-Ag/NiO	552:590	The catalytic activity of the BC-Ag/NiO was evaluated against chemical reduction of congo red, methyl orange and methylene blue.
35304202	1	17	theme	sheets	177:182	arg1	synthesis					130:138	the synthesis	126:138	the synthesis of bacterial cellulose (BC) hydrogel sheets	126:182	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	1	17	theme	sheets	177:182	arg1	utilization					194:204	their utilization	188:204	their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO)	188:265	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	0	18	theme	Efficient	0:8	arg1	fabrication					10:20	Efficient fabrication	0:20	Efficient fabrication	0:20	Efficient fabrication, antibacterial and catalytic performance of Ag-NiO loaded bacterial cellulose paper.
35304202	4	19	theme	release	537:543	arg1	study					545:549	Ni2+ ion release study	528:549	Ni2+ ion release study	528:549	The stability of nanocomposites-Hydrogel was confirmed by Ag+ and Ni2+ ion release study.
35304202	8	20	theme	Gram-negative	1058:1070	arg1	coli					1094:1097	Escherichia coli	1082:1097	Escherichia coli (E.coli)	1082:1106	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	8	20	theme	Gram-negative	1058:1070	arg1	bacteria					1126:1133	Gram-positive bacteria	1112:1133	Gram-positive bacteria	1112:1133	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	8	20	theme	Gram-negative	1058:1070	arg1	aureus					1151:1156	Staphylococcus aureus	1136:1156	Staphylococcus aureus	1136:1156	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	8	20	theme	Gram-negative	1058:1070	arg1	bacteria					1072:1079	Gram-negative bacteria	1058:1079	Gram-negative bacteria	1058:1079	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	6	21	theme	0.1147 min-1	760:771	arg1	values					750:755	kapp values	745:755	kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1	745:803	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	3	22	theme	Ag/NiO	406:411	arg1	composition					413:423	A 20% Ag/NiO composition	400:423	A 20% Ag/NiO composition	400:423	A 20% Ag/NiO composition was revealed by dry weight analysis.
35304202	4	23	theme	ion	533:535	arg1	study					545:549	Ni2+ ion release study	528:549	Ni2+ ion release study	528:549	The stability of nanocomposites-Hydrogel was confirmed by Ag+ and Ni2+ ion release study.
35304202	3	24	theme	dry	441:443	arg1	analysis					452:459	dry weight analysis	441:459	dry weight analysis	441:459	A 20% Ag/NiO composition was revealed by dry weight analysis.
35304202	1	25	theme	BC	164:165	arg1	sheets					177:182	bacterial cellulose (BC) hydrogel sheets	143:182	bacterial cellulose (BC) hydrogel sheets	143:182	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	3	26	theme	weight	445:450	arg1	analysis					452:459	dry weight analysis	441:459	dry weight analysis	441:459	A 20% Ag/NiO composition was revealed by dry weight analysis.
35304202	0	27	theme	catalytic	41:49	arg1	performance					51:61	catalytic performance	41:61	catalytic performance	41:61	Efficient fabrication, antibacterial and catalytic performance of Ag-NiO loaded bacterial cellulose paper.
35304202	6	28	theme	reduction	685:693	arg1	reaction					695:702	The reduction reaction	681:702	The reduction reaction	681:702	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	6	29	theme	MB	835:836	arg1	dyes					838:841	MB dyes	835:841	MB dyes	835:841	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	3	30	theme	20	402:403	arg1	%					404:404	%	404:404	%	404:404	A 20% Ag/NiO composition was revealed by dry weight analysis.
35304202	8	31	theme	synthesized	967:977	arg1	hydrogel					994:1001	The synthesized nanocomposites hydrogel	963:1001	The synthesized nanocomposites hydrogel	963:1001	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	5	32	theme	orange	654:659	arg1	reduction					623:631	chemical reduction	614:631	chemical reduction of congo red, methyl orange and methylene blue	614:678	The catalytic activity of the BC-Ag/NiO was evaluated against chemical reduction of congo red, methyl orange and methylene blue.
35304202	6	33	theme	kapp	745:748	arg1	values					750:755	kapp values	745:755	kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1	745:803	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	4	34	theme	Ni2+	528:531	arg1	study					545:549	Ni2+ ion release study	528:549	Ni2+ ion release study	528:549	The stability of nanocomposites-Hydrogel was confirmed by Ag+ and Ni2+ ion release study.
35304202	8	35	theme	Gram-positive	1112:1124	arg1	bacteria					1126:1133	Gram-positive bacteria	1112:1133	Gram-positive bacteria	1112:1133	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	8	35	theme	Gram-positive	1112:1124	arg1	bacteria					1072:1079	Gram-negative bacteria	1058:1079	Gram-negative bacteria	1058:1079	The synthesized nanocomposites hydrogel was also tested for its antibacterial activity against Gram-negative bacteria, Escherichia coli (E.coli) and Gram-positive bacteria, Staphylococcus aureus (S.aureus).
35304202	2	36	dep	employed	310:317	arg1	followed					350:357	followed	350:357	followed by impregnation into BC hydrogel sheets	350:397	A two-step facile hydrothermal method was employed for the preparation of Ag/NiO, followed by impregnation into BC hydrogel sheets.
35304202	5	37	theme	blue	675:678	arg1	reduction					623:631	chemical reduction	614:631	chemical reduction of congo red, methyl orange and methylene blue	614:678	The catalytic activity of the BC-Ag/NiO was evaluated against chemical reduction of congo red, methyl orange and methylene blue.
35304202	6	38	theme	order	726:730	arg1	kinetics					732:739	pseudo first order kinetics	713:739	pseudo first order kinetics	713:739	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	7	39	theme	BC-Ag/NiO	862:870	arg1	catalyst					872:879	The BC-Ag/NiO catalyst	858:879	The BC-Ag/NiO catalyst	858:879	The BC-Ag/NiO catalyst could be easily recovered and re-used in another reaction without centrifugation.
35304202	6	40	theme	first	720:724	arg1	kinetics					732:739	pseudo first order kinetics	713:739	pseudo first order kinetics	713:739	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	1	41	theme	bacterial	143:151	arg1	sheets					177:182	bacterial cellulose (BC) hydrogel sheets	143:182	bacterial cellulose (BC) hydrogel sheets	143:182	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	2	42	theme	BC	380:381	arg1	sheets					392:397	BC hydrogel sheets	380:397	BC hydrogel sheets	380:397	A two-step facile hydrothermal method was employed for the preparation of Ag/NiO, followed by impregnation into BC hydrogel sheets.
35304202	1	43	theme	silver‑nickel	223:235	arg1	Ag/NiO					259:264	Ag/NiO	259:264	Ag/NiO	259:264	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	1	43	theme	silver‑nickel	223:235	arg1	nanocomposites					243:256	silver‑nickel oxide nanocomposites	223:256	silver‑nickel oxide nanocomposites (Ag/NiO)	223:265	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	0	44	theme	Ag-NiO	66:71	arg1	antibacterial					23:35	antibacterial	23:35	antibacterial	23:35	Efficient fabrication, antibacterial and catalytic performance of Ag-NiO loaded bacterial cellulose paper.
35304202	0	44	theme	Ag-NiO	66:71	arg1	performance					51:61	catalytic performance	41:61	catalytic performance	41:61	Efficient fabrication, antibacterial and catalytic performance of Ag-NiO loaded bacterial cellulose paper.
35304202	0	44	theme	Ag-NiO	66:71	arg1	fabrication					10:20	Efficient fabrication	0:20	Efficient fabrication	0:20	Efficient fabrication, antibacterial and catalytic performance of Ag-NiO loaded bacterial cellulose paper.
35304202	4	45	theme	nanocomposites-Hydrogel	479:501	arg1	stability					466:474	The stability	462:474	The stability of nanocomposites-Hydrogel	462:501	The stability of nanocomposites-Hydrogel was confirmed by Ag+ and Ni2+ ion release study.
35304202	6	46	theme	pseudo	713:718	arg1	kinetics					732:739	pseudo first order kinetics	713:739	pseudo first order kinetics	713:739	The reduction reaction followed pseudo first order kinetics and kapp values of 0.1147 min-1, 0.1323 min-1 and 0.12989 min-1 were obtained for CR, MO, and MB dyes, respectively.
35304202	1	47	theme	cellulose	153:161	arg1	sheets					177:182	bacterial cellulose (BC) hydrogel sheets	143:182	bacterial cellulose (BC) hydrogel sheets	143:182	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	1	48	theme	oxide	237:241	arg1	Ag/NiO					259:264	Ag/NiO	259:264	Ag/NiO	259:264	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35304202	1	48	theme	oxide	237:241	arg1	nanocomposites					243:256	silver‑nickel oxide nanocomposites	223:256	silver‑nickel oxide nanocomposites (Ag/NiO)	223:265	This study reports the synthesis of bacterial cellulose (BC) hydrogel sheets and their utilization as a support for silver‑nickel oxide nanocomposites (Ag/NiO).
35123750	5	0	dep	attachment	642:651	arg1	the					638:640	the	638:640	the	638:640	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	0	1	theme	scaffold	95:102	arg1	Development					0:10	Development	0:10	Development	0:10	Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
35123750	0	1	theme	scaffold	95:102	arg1	assessment					25:34	in vitro assessment	16:34	in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	16:102	Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
35123750	5	2	theme	chondrogenic	818:829	arg1	layer					831:835	the chondrogenic layer	814:835	the chondrogenic layer	814:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	6	3	dep	gradient	987:994	arg1	composition					1009:1019	composition	1009:1019	composition	1009:1019	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	6	4	theme	chondrogenic	853:864	arg1	differentiation					866:880	Osteogenic and chondrogenic differentiation	838:880	differentiation	866:880	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	4	5	theme	instantaneous	509:521	arg1	recovery					534:541	instantaneous mechanical recovery	509:541	instantaneous mechanical recovery	509:541	The scaffold showed instantaneous mechanical recovery under compressive loading and did not delaminate under tensile loading.
35123750	7	6	theme	invasive	1074:1081	arg1	delivery					1096:1103	minimally invasive arthroscopic delivery	1064:1103	minimally invasive arthroscopic delivery	1064:1103	This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.
35123750	6	7	theme	pore	982:985	arg1	gradient					987:994	the distinct pore gradient	969:994	the distinct pore gradient	969:994	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	5	8	theme	mesenchymal	680:690	arg1	cells					697:701	human mesenchymal stem cells	674:701	human mesenchymal stem cells (MSCs)	674:708	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	8	theme	mesenchymal	680:690	arg1	MSCs					704:707	MSCs	704:707	MSCs	704:707	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	9	theme	human	674:678	arg1	cells					697:701	human mesenchymal stem cells	674:701	human mesenchymal stem cells (MSCs)	674:708	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	9	theme	human	674:678	arg1	MSCs					704:707	MSCs	704:707	MSCs	704:707	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	10	located	found	749:753	arg1	layer					767:771	the bone layer	758:771	the bone layer compared to a rounded cell morphology on the chondrogenic layer	758:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	10	located	found	749:753	arg2	morphology					738:747	typical adherent cell morphology	716:747	typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer	716:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	1	11	theme	osteochondral	192:204	arg1	repair					213:218	osteochondral defect repair	192:218	osteochondral defect repair	192:218	An innovative approach was developed to engineer a multi-layered chitosan scaffold for osteochondral defect repair.
35123750	6	12	theme	scaffold	940:947	arg1	layers					926:931	selected layers	917:931	selected layers of the scaffold in vitro	917:956	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	5	13	theme	stem	692:695	arg1	cells					697:701	human mesenchymal stem cells	674:701	human mesenchymal stem cells (MSCs)	674:708	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	13	theme	stem	692:695	arg1	MSCs					704:707	MSCs	704:707	MSCs	704:707	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	1	14	theme	defect	206:211	arg1	repair					213:218	osteochondral defect repair	192:218	osteochondral defect repair	192:218	An innovative approach was developed to engineer a multi-layered chitosan scaffold for osteochondral defect repair.
35123750	3	15	theme	nano-hydroxyapatite	410:428	arg1	Incorporation					386:398	Incorporation	386:398	Incorporation of 70 wt% nano-hydroxyapatite (nHA)	386:434	Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer.
35123750	4	16	theme	mechanical	523:532	arg1	recovery					534:541	instantaneous mechanical recovery	509:541	instantaneous mechanical recovery	509:541	The scaffold showed instantaneous mechanical recovery under compressive loading and did not delaminate under tensile loading.
35123750	5	17	theme	cells	697:701	arg1	proliferation					657:669	proliferation	657:669	proliferation	657:669	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	17	theme	cells	697:701	arg1	attachment					642:651	attachment	642:651	attachment	642:651	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	18	theme	rounded	787:793	arg1	morphology					800:809	a rounded cell morphology	785:809	a rounded cell morphology on the chondrogenic layer	785:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	2	19	dep	porous	296:301	arg1	bioresorbable					304:316	bioresorbable	304:316	bioresorbable	304:316	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	7	20	theme	damaged	1148:1154	arg1	cartilage					1156:1164	damaged cartilage	1148:1164	damaged cartilage	1148:1164	This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.
35123750	1	21	theme	innovative	108:117	arg1	approach					119:126	An innovative approach	105:126	An innovative approach	105:126	An innovative approach was developed to engineer a multi-layered chitosan scaffold for osteochondral defect repair.
35123750	3	22	theme	70 wt	403:407	arg1	nano-hydroxyapatite					410:428	70 wt% nano-hydroxyapatite	403:428	70 wt% nano-hydroxyapatite (nHA)	403:434	Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer.
35123750	3	22	theme	70 wt	403:407	arg1	nHA					431:433	nHA	431:433	nHA	431:433	Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer.
35123750	4	23	theme	tensile	598:604	arg1	loading					606:612	tensile loading	598:612	tensile loading	598:612	The scaffold showed instantaneous mechanical recovery under compressive loading and did not delaminate under tensile loading.
35123750	6	24	theme	Osteogenic	838:847	arg1	differentiation					866:880	Osteogenic and chondrogenic differentiation	838:880	differentiation	866:880	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	2	25	theme	distinct	334:341	arg1	gradient					343:350	a distinct gradient	332:350	a distinct gradient of pore size (mean = 160-275 μm)	332:383	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	7	26	from	candidate	1050:1058	arg1	clinic					1112:1117	the clinic	1108:1117	the clinic with potential to regenerate damaged cartilage and bone	1108:1173	This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.
35123750	0	27	theme	in	16:17	arg1	assessment					25:34	in vitro assessment	16:34	in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	16:102	Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
35123750	6	28	dep	occurred	905:912	arg1	driven					959:964	driven	959:964	driven by the distinct pore gradient and material composition	959:1019	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	3	29	theme	%	408:408	arg1	nano-hydroxyapatite					410:428	70 wt% nano-hydroxyapatite	403:428	70 wt% nano-hydroxyapatite (nHA)	403:434	Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer.
35123750	3	29	theme	%	408:408	arg1	nHA					431:433	nHA	431:433	nHA	431:433	Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer.
35123750	2	30	theme	out	273:275	arg1	methods					277:283	porogen-leaching out methods	256:283	porogen-leaching out methods	256:283	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	3	31	theme	additional	445:454	arg1	strength					456:463	additional strength	445:463	additional strength	445:463	Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer.
35123750	5	32	theme	bone	762:765	arg1	layer					767:771	the bone layer	758:771	the bone layer compared to a rounded cell morphology on the chondrogenic layer	758:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	6	33	theme	selected	917:924	arg1	layers					926:931	selected layers	917:931	selected layers of the scaffold in vitro	917:956	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	2	34	theme	porogen-leaching	256:271	arg1	methods					277:283	porogen-leaching out methods	256:283	porogen-leaching out methods	256:283	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	0	35	theme	bi-layered	41:50	arg1	scaffold					95:102	a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	39:102	a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	39:102	Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
35123750	5	36	theme	typical	716:722	arg1	morphology					738:747	typical adherent cell morphology	716:747	typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer	716:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	37	theme	cell	795:798	arg1	morphology					800:809	a rounded cell morphology	785:809	a rounded cell morphology on the chondrogenic layer	785:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	7	38	theme	arthroscopic	1083:1094	arg1	delivery					1096:1103	minimally invasive arthroscopic delivery	1064:1103	minimally invasive arthroscopic delivery	1064:1103	This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.
35123750	5	39	from	morphology	800:809	arg1	layer					831:835	the chondrogenic layer	814:835	the chondrogenic layer	814:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	5	40	theme	adherent	724:731	arg1	morphology					738:747	typical adherent cell morphology	716:747	typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer	716:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	2	41	theme	freeze	238:243	arg1	drying					245:250	freeze drying	238:250	freeze drying	238:250	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	2	42	theme	drying	245:250	arg1	combination					223:233	A combination	221:233	A combination of freeze drying and porogen-leaching out methods	221:283	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	7	43	with	clinic	1112:1117	arg1	potential					1124:1132	potential	1124:1132	potential	1124:1132	This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.
35123750	6	44	theme	distinct	973:980	arg1	gradient					987:994	the distinct pore gradient	969:994	the distinct pore gradient	969:994	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	5	45	theme	cell	733:736	arg1	morphology					738:747	typical adherent cell morphology	716:747	typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer	716:835	The scaffold supported the attachment and proliferation of human mesenchymal stem cells (MSCs), with typical adherent cell morphology found on the bone layer compared to a rounded cell morphology on the chondrogenic layer.
35123750	2	46	theme	porous	296:301	arg1	scaffold					318:325	a porous, bioresorbable scaffold	294:325	a porous, bioresorbable scaffold	294:325	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	2	47	theme	size	360:363	arg1	gradient					343:350	a distinct gradient	332:350	a distinct gradient of pore size (mean = 160-275 μm)	332:383	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	6	48	theme	MSCs	885:888	arg1	differentiation					866:880	Osteogenic and chondrogenic differentiation	838:880	differentiation	866:880	Osteogenic and chondrogenic differentiation of MSCs preferentially occurred in selected layers of the scaffold in vitro, driven by the distinct pore gradient and material composition.
35123750	0	49	dep	in	16:17	arg1	vitro					19:23	vitro	19:23	vitro	19:23	Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
35123750	2	50	theme	pore	355:358	arg1	mean = 160-275 μm					366:382	mean = 160-275 μm	366:382	mean = 160-275 μm	366:382	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	2	50	theme	pore	355:358	arg1	size					360:363	pore size	355:363	pore size (mean = 160-275 μm)	355:383	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	0	51	theme	osteochondral	81:93	arg1	scaffold					95:102	a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	39:102	a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	39:102	Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
35123750	7	52	theme	suitable	1041:1048	arg1	scaffold					1027:1034	This scaffold	1022:1034	This scaffold	1022:1034	This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.
35123750	7	52	theme	suitable	1041:1048	arg1	candidate					1050:1058	a suitable candidate	1039:1058	a suitable candidate for minimally invasive arthroscopic delivery	1039:1103	This scaffold is a suitable candidate for minimally invasive arthroscopic delivery in the clinic with potential to regenerate damaged cartilage and bone.
35123750	1	53	theme	multi-layered	156:168	arg1	scaffold					179:186	a multi-layered chitosan scaffold	154:186	a multi-layered chitosan scaffold for osteochondral defect repair	154:218	An innovative approach was developed to engineer a multi-layered chitosan scaffold for osteochondral defect repair.
35123750	3	54	theme	bone-like	472:480	arg1	layer					482:486	the bone-like layer	468:486	the bone-like layer	468:486	Incorporation of 70 wt% nano-hydroxyapatite (nHA) provided additional strength to the bone-like layer.
35123750	0	55	theme	chitosan-nano-hydroxyapatite	52:79	arg1	scaffold					95:102	a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	39:102	a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold	39:102	Development and in vitro assessment of a bi-layered chitosan-nano-hydroxyapatite osteochondral scaffold.
35123750	4	56	theme	compressive	549:559	arg1	loading					561:567	compressive loading	549:567	compressive loading	549:567	The scaffold showed instantaneous mechanical recovery under compressive loading and did not delaminate under tensile loading.
35123750	2	57	theme	methods	277:283	arg1	combination					223:233	A combination	221:233	A combination of freeze drying and porogen-leaching out methods	221:283	A combination of freeze drying and porogen-leaching out methods produced a porous, bioresorbable scaffold with a distinct gradient of pore size (mean = 160-275 μm).
35123750	1	58	theme	chitosan	170:177	arg1	scaffold					179:186	a multi-layered chitosan scaffold	154:186	a multi-layered chitosan scaffold for osteochondral defect repair	154:218	An innovative approach was developed to engineer a multi-layered chitosan scaffold for osteochondral defect repair.
35020636	12	0	theme	complication	2017:2028	arg1	episode					1998:2004	1 episode	1996:2004	1 episode of a minor complication such as bradycardia or bronchospasm	1996:2064	Since 250 patients would need to be given neostigmine rather than sugammadex to avoid 1 episode of a minor complication such as bradycardia or bronchospasm, we conclude that sugammadex and neostigmine are comparably safe.
35020636	3	1	theme	patients	518:525	arg1	records					507:513	medical records	499:513	medical records of patients who had general, cardiothoracic, or pediatric surgery	499:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	12	2	theme	minor	2011:2015	arg1	complication					2017:2028	a minor complication	2009:2028	a minor complication such as bradycardia or bronchospasm	2009:2064	Since 250 patients would need to be given neostigmine rather than sugammadex to avoid 1 episode of a minor complication such as bradycardia or bronchospasm, we conclude that sugammadex and neostigmine are comparably safe.
35020636	6	3	theme	odds	1078:1081	arg1	ratio					1083:1087	the odds ratio	1074:1087	the odds ratio for composite of side effects	1074:1117	Sugammadex would be considered noninferior to neostigmine (or vice versa) if the odds ratio for composite of side effects did not exceed 1.2.
35020636	6	4	dep	neostigmine	1043:1053	arg1	versa					1064:1068	versa	1064:1068	versa	1064:1068	Sugammadex would be considered noninferior to neostigmine (or vice versa) if the odds ratio for composite of side effects did not exceed 1.2.
35020636	6	5	theme	side	1106:1109	arg1	effects					1111:1117	side effects	1106:1117	side effects	1106:1117	Sugammadex would be considered noninferior to neostigmine (or vice versa) if the odds ratio for composite of side effects did not exceed 1.2.
35020636	0	6	theme	Retrospective	93:105	arg1	Analysis					114:121	A Retrospective Cohort Analysis	91:121	A Retrospective Cohort Analysis of Postoperative Side Effects	91:151	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	3	7	contain	had	531:533	arg2	surgery					573:579	general, cardiothoracic, or pediatric surgery	535:579	general, cardiothoracic, or pediatric surgery	535:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	3	7	contain	had	531:533	arg1	patients					518:525	patients	518:525	patients who had general, cardiothoracic, or pediatric surgery	518:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	10	8	theme	noninferiority	1630:1643	arg1	P					1645:1645	noninferiority P	1630:1645	noninferiority P = .57	1630:1651	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	5	9	dep	priori	862:867	arg1	We					857:858	We	857:858	We	857:858	We a priori restricted our analysis to side effects requiring pharmacologic treatment that were therefore presumably clinically meaningful.
35020636	5	10	theme	side	896:899	arg1	effects					901:907	side effects	896:907	side effects requiring pharmacologic treatment that were therefore presumably clinically meaningful	896:994	We a priori restricted our analysis to side effects requiring pharmacologic treatment that were therefore presumably clinically meaningful.
35020636	4	11	theme	cardiac	748:754	arg1	arrest					756:761	cardiac arrest	748:761	cardiac arrest	748:761	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	10	12	with	sugammadex	1687:1696	arg1	ratio					1721:1725	an estimated odds ratio	1703:1725	an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001	1703:1774	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	10	13	theme	odds	1538:1541	arg1	ratio					1543:1547	an estimated odds ratio	1525:1547	an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34	1525:1627	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	10	14	theme	=	1647:1647	arg1	P					1645:1645	noninferiority P	1630:1645	noninferiority P = .57	1630:1651	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	4	15	theme	bradycardia	704:714	arg1	anaphylaxis					717:727	anaphylaxis	717:727	anaphylaxis	717:727	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	15	theme	bradycardia	704:714	arg1	bronchospasm					730:741	bronchospasm	730:741	bronchospasm	730:741	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	15	theme	bradycardia	704:714	arg1	composite					691:699	a collapsed composite	679:699	a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room	679:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	15	theme	bradycardia	704:714	arg1	bradycardia					704:714	bradycardia	704:714	bradycardia	704:714	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	15	theme	bradycardia	704:714	arg1	outcome					667:673	Our primary outcome	655:673	Our primary outcome	655:673	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	15	theme	bradycardia	704:714	arg1	arrest					756:761	cardiac arrest	748:761	cardiac arrest	748:761	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	9	16	theme	common	1388:1393	arg1	effect					1411:1416	The most common individual side effect	1379:1416	The most common individual side effect	1379:1416	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	9	16	theme	common	1388:1393	arg1	bradycardia					1422:1432	bradycardia	1422:1432	bradycardia	1422:1432	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	5	17	theme	pharmacologic	919:931	arg1	treatment					933:941	pharmacologic treatment	919:941	pharmacologic treatment that were therefore presumably clinically meaningful	919:994	We a priori restricted our analysis to side effects requiring pharmacologic treatment that were therefore presumably clinically meaningful.
35020636	6	18	theme	effects	1111:1117	arg1	composite					1093:1101	composite	1093:1101	composite of side effects	1093:1117	Sugammadex would be considered noninferior to neostigmine (or vice versa) if the odds ratio for composite of side effects did not exceed 1.2.
35020636	6	18	theme	effects	1111:1117	arg1	effects					1111:1117	side effects	1106:1117	side effects	1106:1117	Sugammadex would be considered noninferior to neostigmine (or vice versa) if the odds ratio for composite of side effects did not exceed 1.2.
35020636	2	19	theme	meaningful	439:448	arg1	effects					455:461	clinically meaningful side effects	428:461	clinically meaningful side effects	428:461	We tested the hypothesis that sugammadex is noninferior to neostigmine on a composite of clinically meaningful side effects, or vice versa.
35020636	3	20	from	June	627:630	arg1	sugammadex					611:620	sugammadex	611:620	sugammadex	611:620	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	3	20	from	June	627:630	arg1	METHODS					479:485	METHODS	479:485	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.	479:653	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	3	20	from	June	627:630	arg1	neostigmine					596:606	neostigmine	596:606	neostigmine	596:606	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	9	21	theme	individual	1395:1404	arg1	effect					1411:1416	The most common individual side effect	1379:1416	The most common individual side effect	1379:1416	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	9	21	theme	individual	1395:1404	arg1	bradycardia					1422:1432	bradycardia	1422:1432	bradycardia	1422:1432	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	0	22	theme	Cohort	107:112	arg1	Analysis					114:121	A Retrospective Cohort Analysis	91:121	A Retrospective Cohort Analysis of Postoperative Side Effects	91:151	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	3	23	theme	cardiothoracic	544:557	arg1	surgery					573:579	general, cardiothoracic, or pediatric surgery	535:579	general, cardiothoracic, or pediatric surgery	535:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	4	24	theme	primary	659:665	arg1	anaphylaxis					717:727	anaphylaxis	717:727	anaphylaxis	717:727	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	24	theme	primary	659:665	arg1	bronchospasm					730:741	bronchospasm	730:741	bronchospasm	730:741	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	24	theme	primary	659:665	arg1	composite					691:699	a collapsed composite	679:699	a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room	679:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	24	theme	primary	659:665	arg1	bradycardia					704:714	bradycardia	704:714	bradycardia	704:714	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	24	theme	primary	659:665	arg1	outcome					667:673	Our primary outcome	655:673	Our primary outcome	655:673	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	24	theme	primary	659:665	arg1	arrest					756:761	cardiac arrest	748:761	cardiac arrest	748:761	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	25	from	room	851:854	arg1	administration					781:794	administration	781:794	administration of the reversal agent	781:816	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	25	from	room	851:854	arg1	departure					822:830	departure	822:830	departure from the operation room	822:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	11	26	theme	composite	1793:1801	arg1	less					1817:1820	less	1817:1820	less	1817:1820	CONCLUSIONS The composite incidence was less with neostigmine than with sugammadex, but only by 0.4% (a negligible clinical effect).
35020636	11	26	theme	composite	1793:1801	arg1	incidence					1803:1811	The composite incidence	1789:1811	The composite incidence	1789:1811	CONCLUSIONS The composite incidence was less with neostigmine than with sugammadex, but only by 0.4% (a negligible clinical effect).
35020636	7	27	from	surgeries	1160:1168	arg1	patients					1180:1187	70,690 patients	1173:1187	70,690 patients	1173:1187	RESULTS Among 89,753 surgeries in 70,690 patients, 16,480 (18%) were given sugammadex and 73,273 (82%) were given neostigmine.
35020636	7	27	from	surgeries	1160:1168	arg1	16,480					1190:1195	16,480	1190:1195	16,480	1190:1195	RESULTS Among 89,753 surgeries in 70,690 patients, 16,480 (18%) were given sugammadex and 73,273 (82%) were given neostigmine.
35020636	10	28	theme	superiority	1755:1765	arg1	P					1767:1767	1-side superiority P <	1748:1769	1-side superiority P < .001	1748:1774	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	10	28	theme	superiority	1755:1765	arg1	0.83					1730:1733	0.83	1730:1733	0.83	1730:1733	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	11	29	theme	negligible	1881:1890	arg1	effect					1901:1906	a negligible clinical effect	1879:1906	a negligible clinical effect	1879:1906	CONCLUSIONS The composite incidence was less with neostigmine than with sugammadex, but only by 0.4% (a negligible clinical effect).
35020636	10	30	theme	estimated	1528:1536	arg1	ratio					1543:1547	an estimated odds ratio	1525:1547	an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34	1525:1627	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	9	31	dep	bradycardia	1422:1432	arg1	%					1474:1474	2.2%	1471:1474	2.2%	1471:1474	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	9	31	dep	bradycardia	1422:1432	arg1	%					1438:1438	2.4%	1435:1438	2.4% in the sugammadex group	1435:1462	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	0	32	theme	Postoperative	126:138	arg1	Effects					145:151	Postoperative Side Effects	126:151	Postoperative Side Effects	126:151	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	3	33	theme	pediatric	563:571	arg1	surgery					573:579	general, cardiothoracic, or pediatric surgery	535:579	general, cardiothoracic, or pediatric surgery	535:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	2	34	theme	side	450:453	arg1	effects					455:461	clinically meaningful side effects	428:461	clinically meaningful side effects	428:461	We tested the hypothesis that sugammadex is noninferior to neostigmine on a composite of clinically meaningful side effects, or vice versa.
35020636	2	35	theme	effects	455:461	arg1	effects					455:461	clinically meaningful side effects	428:461	clinically meaningful side effects	428:461	We tested the hypothesis that sugammadex is noninferior to neostigmine on a composite of clinically meaningful side effects, or vice versa.
35020636	2	35	theme	effects	455:461	arg1	composite					415:423	a composite	413:423	a composite of clinically meaningful side effects	413:461	We tested the hypothesis that sugammadex is noninferior to neostigmine on a composite of clinically meaningful side effects, or vice versa.
35020636	9	36	theme	sugammadex	1447:1456	arg1	group					1458:1462	the sugammadex group	1443:1462	the sugammadex group	1443:1462	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	10	37	theme	1-side	1748:1753	arg1	P					1767:1767	1-side superiority P <	1748:1769	1-side superiority P < .001	1748:1774	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	10	37	theme	1-side	1748:1753	arg1	0.83					1730:1733	0.83	1730:1733	0.83	1730:1733	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	0	38	dep	Blocks	69:74	arg1	Analysis					114:121	A Retrospective Cohort Analysis	91:121	A Retrospective Cohort Analysis of Postoperative Side Effects	91:151	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	1	39	theme	neuromuscular	218:230	arg1	blockade					232:239	residual neuromuscular blockade	209:239	residual neuromuscular blockade	209:239	BACKGROUND Sugammadex and neostigmine given to reverse residual neuromuscular blockade can cause side effects including bradycardia, anaphylaxis, bronchospasm, and even cardiac arrest.
35020636	1	40	theme	even	318:321	arg1	arrest					331:336	even cardiac arrest	318:336	even cardiac arrest	318:336	BACKGROUND Sugammadex and neostigmine given to reverse residual neuromuscular blockade can cause side effects including bradycardia, anaphylaxis, bronchospasm, and even cardiac arrest.
35020636	0	41	theme	Effects	145:151	arg1	Analysis					114:121	A Retrospective Cohort Analysis	91:121	A Retrospective Cohort Analysis of Postoperative Side Effects	91:151	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	10	42	theme	confidence	1593:1602	arg1	interval					1604:1611	95% confidence interval [CI	1589:1615	95% confidence interval [CI	1589:1615	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	10	43	theme	odds	1716:1719	arg1	ratio					1721:1725	an estimated odds ratio	1703:1725	an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001	1703:1774	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	3	44	dep	June	627:630	arg1	December					640:647	December	640:647	December	640:647	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	3	44	dep	June	627:630	arg1	to					637:638	to	637:638	to	637:638	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	11	45	dep	less	1817:1820	arg1	effect					1901:1906	a negligible clinical effect	1879:1906	a negligible clinical effect	1879:1906	CONCLUSIONS The composite incidence was less with neostigmine than with sugammadex, but only by 0.4% (a negligible clinical effect).
35020636	1	46	theme	cardiac	323:329	arg1	arrest					331:336	even cardiac arrest	318:336	even cardiac arrest	318:336	BACKGROUND Sugammadex and neostigmine given to reverse residual neuromuscular blockade can cause side effects including bradycardia, anaphylaxis, bronchospasm, and even cardiac arrest.
35020636	0	47	theme	Side	140:143	arg1	Effects					145:151	Postoperative Side Effects	126:151	Postoperative Side Effects	126:151	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	8	48	theme	composite	1283:1291	arg1	outcome					1293:1299	composite outcome	1283:1299	composite outcome	1283:1299	The incidence of composite outcome was 3.4% in patients given sugammadex and 3.0% in patients given neostigmine.
35020636	10	49	theme	estimated	1706:1714	arg1	ratio					1721:1725	an estimated odds ratio	1703:1725	an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001	1703:1774	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	11	50	theme	clinical	1892:1899	arg1	effect					1901:1906	a negligible clinical effect	1879:1906	a negligible clinical effect	1879:1906	CONCLUSIONS The composite incidence was less with neostigmine than with sugammadex, but only by 0.4% (a negligible clinical effect).
35020636	5	51	theme	a	860:860	arg1	priori					862:867	We a priori	857:867	We a priori	857:867	We a priori restricted our analysis to side effects requiring pharmacologic treatment that were therefore presumably clinically meaningful.
35020636	0	52	theme	Neuromuscular	55:67	arg1	Reversal					34:41	Reversal	34:41	Reversal of Residual Neuromuscular	34:67	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	9	53	theme	side	1406:1409	arg1	effect					1411:1416	The most common individual side effect	1379:1416	The most common individual side effect	1379:1416	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	9	53	theme	side	1406:1409	arg1	bradycardia					1422:1432	bradycardia	1422:1432	bradycardia	1422:1432	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	4	54	theme	operation	841:849	arg1	room					851:854	the operation room	837:854	the operation room	837:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	9	55	from	%	1438:1438	arg1	group					1458:1462	the sugammadex group	1443:1462	the sugammadex group	1443:1462	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	4	56	theme	anaphylaxis	717:727	arg1	anaphylaxis					717:727	anaphylaxis	717:727	anaphylaxis	717:727	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	56	theme	anaphylaxis	717:727	arg1	bronchospasm					730:741	bronchospasm	730:741	bronchospasm	730:741	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	56	theme	anaphylaxis	717:727	arg1	composite					691:699	a collapsed composite	679:699	a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room	679:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	56	theme	anaphylaxis	717:727	arg1	bradycardia					704:714	bradycardia	704:714	bradycardia	704:714	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	56	theme	anaphylaxis	717:727	arg1	outcome					667:673	Our primary outcome	655:673	Our primary outcome	655:673	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	56	theme	anaphylaxis	717:727	arg1	arrest					756:761	cardiac arrest	748:761	cardiac arrest	748:761	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	0	57	theme	Residual	46:53	arg1	Neuromuscular					55:67	Residual Neuromuscular	46:67	Residual Neuromuscular	46:67	Sugammadex Versus Neostigmine for Reversal of Residual Neuromuscular Blocks After Surgery: A Retrospective Cohort Analysis of Postoperative Side Effects.
35020636	10	58	dep	found	1513:1517	arg1	P					1645:1645	noninferiority P	1630:1645	noninferiority P = .57	1630:1651	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	1	59	theme	BACKGROUND	154:163	arg1	Sugammadex					165:174	BACKGROUND Sugammadex	154:174	BACKGROUND Sugammadex	154:174	BACKGROUND Sugammadex and neostigmine given to reverse residual neuromuscular blockade can cause side effects including bradycardia, anaphylaxis, bronchospasm, and even cardiac arrest.
35020636	10	60	dep	sugammadex	1558:1567	arg1	interval					1604:1611	95% confidence interval [CI	1589:1615	95% confidence interval [CI	1589:1615	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	11	61	dep	CONCLUSIONS	1777:1787	arg1	less					1817:1820	less	1817:1820	less	1817:1820	CONCLUSIONS The composite incidence was less with neostigmine than with sugammadex, but only by 0.4% (a negligible clinical effect).
35020636	11	61	dep	CONCLUSIONS	1777:1787	arg1	incidence					1803:1811	The composite incidence	1789:1811	The composite incidence	1789:1811	CONCLUSIONS The composite incidence was less with neostigmine than with sugammadex, but only by 0.4% (a negligible clinical effect).
35020636	4	62	theme	reversal	803:810	arg1	agent					812:816	the reversal agent	799:816	the reversal agent	799:816	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	63	theme	collapsed	681:689	arg1	anaphylaxis					717:727	anaphylaxis	717:727	anaphylaxis	717:727	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	63	theme	collapsed	681:689	arg1	bronchospasm					730:741	bronchospasm	730:741	bronchospasm	730:741	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	63	theme	collapsed	681:689	arg1	composite					691:699	a collapsed composite	679:699	a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room	679:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	63	theme	collapsed	681:689	arg1	bradycardia					704:714	bradycardia	704:714	bradycardia	704:714	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	63	theme	collapsed	681:689	arg1	outcome					667:673	Our primary outcome	655:673	Our primary outcome	655:673	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	63	theme	collapsed	681:689	arg1	arrest					756:761	cardiac arrest	748:761	cardiac arrest	748:761	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	64	theme	bronchospasm	730:741	arg1	anaphylaxis					717:727	anaphylaxis	717:727	anaphylaxis	717:727	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	64	theme	bronchospasm	730:741	arg1	bronchospasm					730:741	bronchospasm	730:741	bronchospasm	730:741	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	64	theme	bronchospasm	730:741	arg1	composite					691:699	a collapsed composite	679:699	a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room	679:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	64	theme	bronchospasm	730:741	arg1	bradycardia					704:714	bradycardia	704:714	bradycardia	704:714	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	64	theme	bronchospasm	730:741	arg1	outcome					667:673	Our primary outcome	655:673	Our primary outcome	655:673	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	64	theme	bronchospasm	730:741	arg1	arrest					756:761	cardiac arrest	748:761	cardiac arrest	748:761	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	65	theme	arrest	756:761	arg1	anaphylaxis					717:727	anaphylaxis	717:727	anaphylaxis	717:727	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	65	theme	arrest	756:761	arg1	bronchospasm					730:741	bronchospasm	730:741	bronchospasm	730:741	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	65	theme	arrest	756:761	arg1	composite					691:699	a collapsed composite	679:699	a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room	679:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	65	theme	arrest	756:761	arg1	bradycardia					704:714	bradycardia	704:714	bradycardia	704:714	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	65	theme	arrest	756:761	arg1	outcome					667:673	Our primary outcome	655:673	Our primary outcome	655:673	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	65	theme	arrest	756:761	arg1	arrest					756:761	cardiac arrest	748:761	cardiac arrest	748:761	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	10	66	dep	=	1647:1647	arg1	.57					1649:1651	.57	1649:1651	.57	1649:1651	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	9	67	dep	%	1438:1438	arg1	neostigmine					1476:1486	neostigmine	1476:1486	neostigmine	1476:1486	The most common individual side effect was bradycardia (2.4% in the sugammadex group versus 2.2% neostigmine).
35020636	3	68	theme	medical	499:505	arg1	records					507:513	medical records	499:513	medical records of patients who had general, cardiothoracic, or pediatric surgery	499:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	1	69	theme	side	251:254	arg1	effects					256:262	side effects	251:262	side effects	251:262	BACKGROUND Sugammadex and neostigmine given to reverse residual neuromuscular blockade can cause side effects including bradycardia, anaphylaxis, bronchospasm, and even cardiac arrest.
35020636	10	70	theme	%	1591:1591	arg1	interval					1604:1611	95% confidence interval [CI	1589:1615	95% confidence interval [CI	1589:1615	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	3	71	dep	METHODS	479:485	arg1	analyzed					490:497	analyzed	490:497	analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery	490:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	3	71	dep	METHODS	479:485	arg1	METHODS					479:485	METHODS	479:485	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.	479:653	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	3	71	dep	METHODS	479:485	arg1	neostigmine					596:606	neostigmine	596:606	neostigmine	596:606	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	1	72	theme	residual	209:216	arg1	blockade					232:239	residual neuromuscular blockade	209:239	residual neuromuscular blockade	209:239	BACKGROUND Sugammadex and neostigmine given to reverse residual neuromuscular blockade can cause side effects including bradycardia, anaphylaxis, bronchospasm, and even cardiac arrest.
35020636	8	73	theme	outcome	1293:1299	arg1	incidence					1270:1278	The incidence	1266:1278	The incidence of composite outcome	1266:1299	The incidence of composite outcome was 3.4% in patients given sugammadex and 3.0% in patients given neostigmine.
35020636	8	73	theme	outcome	1293:1299	arg1	%					1308:1308	3.4%	1305:1308	3.4% in patients given sugammadex	1305:1337	The incidence of composite outcome was 3.4% in patients given sugammadex and 3.0% in patients given neostigmine.
35020636	8	74	from	%	1308:1308	arg1	patients					1313:1320	patients	1313:1320	patients given sugammadex	1313:1337	The incidence of composite outcome was 3.4% in patients given sugammadex and 3.0% in patients given neostigmine.
35020636	8	74	from	%	1308:1308	arg1	patients					1351:1358	patients	1351:1358	patients given neostigmine	1351:1376	The incidence of composite outcome was 3.4% in patients given sugammadex and 3.0% in patients given neostigmine.
35020636	10	75	theme	1.21	1552:1555	arg1	ratio					1543:1547	an estimated odds ratio	1525:1547	an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34	1525:1627	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35020636	4	76	theme	agent	812:816	arg1	administration					781:794	administration	781:794	administration of the reversal agent	781:816	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	4	76	theme	agent	812:816	arg1	departure					822:830	departure	822:830	departure from the operation room	822:854	Our primary outcome was a collapsed composite of bradycardia, anaphylaxis, bronchospasm, and cardiac arrest occurring between administration of the reversal agent and departure from the operation room.
35020636	3	77	theme	general	535:541	arg1	surgery					573:579	general, cardiothoracic, or pediatric surgery	535:579	general, cardiothoracic, or pediatric surgery	535:579	METHODS We analyzed medical records of patients who had general, cardiothoracic, or pediatric surgery and were given neostigmine or sugammadex from June 2016 to December 2019.
35020636	8	78	from	%	1346:1346	arg1	patients					1313:1320	patients	1313:1320	patients given sugammadex	1313:1337	The incidence of composite outcome was 3.4% in patients given sugammadex and 3.0% in patients given neostigmine.
35020636	8	78	from	%	1346:1346	arg1	patients					1351:1358	patients	1351:1358	patients given neostigmine	1351:1376	The incidence of composite outcome was 3.4% in patients given sugammadex and 3.0% in patients given neostigmine.
35020636	10	79	theme	0.83	1730:1733	arg1	ratio					1721:1725	an estimated odds ratio	1703:1725	an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001	1703:1774	Noninferiority was not found, with an estimated odds ratio of 1.21 (sugammadex versus neostigmine; 95% confidence interval [CI], 1.09-1.34; noninferiority P = .57), and neostigmine was superior to sugammadex with an estimated odds ratio of 0.83 (0.74-0.92), 1-side superiority P < .001.
35445411	16	0	theme	high	1762:1765	arg1	resistance					1767:1776	high resistance	1762:1776	high resistance to digestion	1762:1789	The Wx-AB in native starch and Wx-Ab in gelatinized starch can control obesity and diabetes by slow-digesting carbohydrates and high resistance to digestion.
35445411	6	1	dep	RESULTS	658:664	arg1	proportion					670:679	The proportion	666:679	RESULTS The proportion of B-type granules	658:698	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	6	2	theme	granules	691:698	arg1	proportion					670:679	The proportion	666:679	RESULTS The proportion of B-type granules	658:698	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	5	3	with	mutants	611:617	arg1	combinations					644:655	different Waxy gene combinations	624:655	different Waxy gene combinations	624:655	Wild-type tetraploid wheat was compared with three mutants with different Waxy gene combinations.
35445411	15	4	theme	waxy	1576:1579	arg1	composition					1581:1591	waxy composition	1576:1591	waxy composition	1576:1591	The lines with differing waxy composition had different effects on food digestion.
35445411	10	5	theme	digestible	1125:1134	arg1	SDS					1144:1146	SDS	1144:1146	SDS	1144:1146	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	10	5	theme	digestible	1125:1134	arg1	starch					1136:1141	slowly digestible starch	1118:1141	slowly digestible starch (SDS)	1118:1147	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	14	6	theme	starch	1543:1548	arg1	chains					1533:1538	the smaller and longer chains	1510:1538	the smaller and longer chains of starch	1510:1548	Waxy proteins significantly affected the smaller and longer chains of starch.
35445411	6	7	theme	significant	772:782	arg1	changes					784:790	significant changes	772:790	significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab)	772:870	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	9	8	theme	order	1073:1077	arg1	Wx-ab>Wx-Ab>Wx-aB>Wx-AB					1079:1101	the order Wx-ab>Wx-Ab>Wx-aB>Wx-AB	1069:1101	the order Wx-ab>Wx-Ab>Wx-aB>Wx-AB	1069:1101	The crystallinity trend followed the order Wx-ab>Wx-Ab>Wx-aB>Wx-AB.
35445411	1	9	theme	increased	153:161	arg1	demand					163:168	An increased demand	150:168	An increased demand for food	150:177	BACKGROUND An increased demand for food has mirrored the increasing global population.
35445411	11	10	theme	native	1296:1301	arg1	starch					1303:1308	native starch	1296:1308	native starch	1296:1308	The amount of retrograded starch was closer to gelatinized starch than to native starch.
35445411	4	11	theme	structural	472:481	arg1	properties					498:507	the structural and functional properties	468:507	the structural and functional properties of starch	468:517	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	5	12	theme	different	624:632	arg1	combinations					644:655	different Waxy gene combinations	624:655	different Waxy gene combinations	624:655	Wild-type tetraploid wheat was compared with three mutants with different Waxy gene combinations.
35445411	5	13	theme	Wild-type	560:568	arg1	wheat					581:585	Wild-type tetraploid wheat	560:585	Wild-type tetraploid wheat	560:585	Wild-type tetraploid wheat was compared with three mutants with different Waxy gene combinations.
35445411	2	14	theme	eating	281:286	arg1	choices					288:294	poor eating choices	276:294	poor eating choices	276:294	Obesity and diabetes are two disorders induced by poor eating choices.
35445411	7	15	theme	chain	891:895	arg1	length					897:902	The lowest branch chain length	873:902	The lowest branch chain length	873:902	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	16	16	theme	native	1647:1652	arg1	starch					1654:1659	native starch	1647:1659	native starch	1647:1659	The Wx-AB in native starch and Wx-Ab in gelatinized starch can control obesity and diabetes by slow-digesting carbohydrates and high resistance to digestion.
35445411	3	17	theme	modified	346:353	arg1	foods					355:359	modified foods	346:359	modified foods that can ameliorate such illnesses	346:394	Consequently, there is an urgent need to develop modified foods that can ameliorate such illnesses.
35445411	12	18	theme	CONCLUSION	1311:1320	arg1	proteins					1327:1334	CONCLUSION Waxy proteins	1311:1334	CONCLUSION Waxy proteins	1311:1334	CONCLUSION Waxy proteins make a substantial contribution to starch structure.
35445411	5	19	theme	Waxy	634:637	arg1	combinations					644:655	different Waxy gene combinations	624:655	different Waxy gene combinations	624:655	Wild-type tetraploid wheat was compared with three mutants with different Waxy gene combinations.
35445411	5	20	theme	tetraploid	570:579	arg1	wheat					581:585	Wild-type tetraploid wheat	560:585	Wild-type tetraploid wheat	560:585	Wild-type tetraploid wheat was compared with three mutants with different Waxy gene combinations.
35445411	2	21	theme	poor	276:279	arg1	choices					288:294	poor eating choices	276:294	poor eating choices	276:294	Obesity and diabetes are two disorders induced by poor eating choices.
35445411	6	22	theme	B-type	684:689	arg1	granules					691:698	B-type granules	684:698	B-type granules	684:698	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	4	23	theme	food	546:549	arg1	quality					551:557	food quality	546:557	food quality	546:557	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	14	24	theme	longer	1526:1531	arg1	chains					1533:1538	the smaller and longer chains	1510:1538	the smaller and longer chains of starch	1510:1548	Waxy proteins significantly affected the smaller and longer chains of starch.
35445411	12	25	theme	substantial	1343:1353	arg1	contribution					1355:1366	a substantial contribution	1341:1366	a substantial contribution to starch structure	1341:1386	CONCLUSION Waxy proteins make a substantial contribution to starch structure.
35445411	15	26	theme	different	1597:1605	arg1	effects					1607:1613	different effects	1597:1613	different effects	1597:1613	The lines with differing waxy composition had different effects on food digestion.
35445411	7	27	located	observed	908:915	arg1	Wx-ab					920:924	Wx-ab	920:924	Wx-ab	920:924	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	7	27	located	observed	908:915	arg2	length					897:902	The lowest branch chain length	873:902	The lowest branch chain length	873:902	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	11	28	theme	retrograded	1236:1246	arg1	starch					1248:1253	retrograded starch	1236:1253	retrograded starch	1236:1253	The amount of retrograded starch was closer to gelatinized starch than to native starch.
35445411	6	29	from	changes	784:790	arg1	Wx-ab					865:869	Wx-ab	865:869	Wx-ab	865:869	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	6	29	from	changes	784:790	arg1	number					820:825	number	820:825	number	820:825	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	6	29	from	changes	784:790	arg1	mutant					857:862	the Wx free mutant	845:862	the Wx free mutant (Wx-ab)	845:870	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	6	29	from	changes	784:790	arg1	phenotype					832:840	phenotype	832:840	phenotype	832:840	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	6	29	from	changes	784:790	arg1	size					814:817	the starch granule size	795:817	the starch granule size	795:817	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	4	30	theme	genes	459:463	arg1	effect					444:449	the effect	440:449	the effect of Waxy genes on the structural and functional properties of starch	440:517	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	11	31	theme	starch	1248:1253	arg1	closer					1259:1264	closer	1259:1264	closer	1259:1264	The amount of retrograded starch was closer to gelatinized starch than to native starch.
35445411	11	31	theme	starch	1248:1253	arg1	amount					1226:1231	The amount	1222:1231	The amount of retrograded starch	1222:1253	The amount of retrograded starch was closer to gelatinized starch than to native starch.
35445411	11	31	theme	starch	1248:1253	arg1	starch					1248:1253	retrograded starch	1236:1253	retrograded starch	1236:1253	The amount of retrograded starch was closer to gelatinized starch than to native starch.
35445411	10	32	theme	gelatinized	1171:1181	arg1	starch					1200:1205	native, gelatinized, and retrograded starch	1163:1205	native, gelatinized, and retrograded starch	1163:1205	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	17	33	theme	Chemical	1810:1817	arg1	Industry					1819:1826	Chemical Industry	1810:1826	Chemical Industry	1810:1826	© 2022 Society of Chemical Industry.
35445411	4	34	theme	Waxy	454:457	arg1	genes					459:463	Waxy genes	454:463	Waxy genes	454:463	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	8	35	theme	crystallinity	1021:1033	arg1	degree					1011:1016	the highest degree	999:1016	the highest degree of crystallinity	999:1033	Wx-ab had the highest degree of crystallinity.
35445411	0	36	dep	Triticum	76:83	arg1	L.					94:95	Triticum turgidum L.	76:95	Triticum turgidum L.	76:95	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	7	37	contain	had	941:943	arg2	length					970:975	the highest branch chain length	945:975	the highest branch chain length of DP ≥ 37	945:986	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	7	37	contain	had	941:943	arg1	Wx-AB					935:939	Wx-AB	935:939	Wx-AB	935:939	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	4	38	from	effect	444:449	arg1	properties					498:507	the structural and functional properties	468:507	the structural and functional properties of starch	468:517	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	13	39	theme	waxy	1399:1402	arg1	proteins					1404:1411	waxy proteins	1399:1411	waxy proteins	1399:1411	A lack of waxy proteins reduced the unit chains markedly compared with the control.
35445411	0	40	theme	structure	19:27	arg1	Analysis					0:7	Analysis	0:7	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.	0:137	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	0	41	theme	waxy	113:116	arg1	composition					126:136	waxy protein composition	113:136	waxy protein composition	113:136	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	14	42	theme	Waxy	1473:1476	arg1	proteins					1478:1485	Waxy proteins	1473:1485	Waxy proteins	1473:1485	Waxy proteins significantly affected the smaller and longer chains of starch.
35445411	0	43	theme	starch	12:17	arg1	structure					19:27	starch structure	12:27	starch structure	12:27	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	11	44	theme	gelatinized	1269:1279	arg1	starch					1281:1286	gelatinized starch	1269:1286	gelatinized starch	1269:1286	The amount of retrograded starch was closer to gelatinized starch than to native starch.
35445411	1	45	dep	BACKGROUND	139:148	arg1	mirrored					183:190	mirrored	183:190	has mirrored the increasing global population	179:223	BACKGROUND An increased demand for food has mirrored the increasing global population.
35445411	0	46	theme	functional	33:42	arg1	properties					44:53	functional properties	33:53	functional properties	33:53	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	7	47	theme	highest	949:955	arg1	length					970:975	the highest branch chain length	945:975	the highest branch chain length of DP ≥ 37	945:986	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	6	48	theme	granule	806:812	arg1	size					814:817	the starch granule size	795:817	the starch granule size	795:817	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	15	49	contain	had	1593:1595	arg2	effects					1607:1613	different effects	1597:1613	different effects	1597:1613	The lines with differing waxy composition had different effects on food digestion.
35445411	15	49	contain	had	1593:1595	arg1	lines					1555:1559	The lines	1551:1559	The lines with differing waxy composition	1551:1591	The lines with differing waxy composition had different effects on food digestion.
35445411	10	50	theme	retrograded	1188:1198	arg1	starch					1200:1205	native, gelatinized, and retrograded starch	1163:1205	native, gelatinized, and retrograded starch	1163:1205	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	1	51	theme	increasing	196:205	arg1	population					214:223	the increasing global population	192:223	the increasing global population	192:223	BACKGROUND An increased demand for food has mirrored the increasing global population.
35445411	0	52	theme	protein	118:124	arg1	composition					126:136	waxy protein composition	113:136	waxy protein composition	113:136	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	6	53	theme	starch	799:804	arg1	size					814:817	the starch granule size	795:817	the starch granule size	795:817	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	12	54	theme	Waxy	1322:1325	arg1	proteins					1327:1334	CONCLUSION Waxy proteins	1311:1334	CONCLUSION Waxy proteins	1311:1334	CONCLUSION Waxy proteins make a substantial contribution to starch structure.
35445411	1	55	theme	global	207:212	arg1	population					214:223	the increasing global population	192:223	the increasing global population	192:223	BACKGROUND An increased demand for food has mirrored the increasing global population.
35445411	3	56	theme	such	381:384	arg1	illnesses					386:394	such illnesses	381:394	such illnesses	381:394	Consequently, there is an urgent need to develop modified foods that can ameliorate such illnesses.
35445411	4	57	theme	functional	487:496	arg1	properties					498:507	the structural and functional properties	468:507	the structural and functional properties of starch	468:517	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	5	58	theme	gene	639:642	arg1	combinations					644:655	different Waxy gene combinations	624:655	different Waxy gene combinations	624:655	Wild-type tetraploid wheat was compared with three mutants with different Waxy gene combinations.
35445411	0	59	theme	tetraploid	58:67	arg1	Triticum					76:83	Triticum	76:83	Triticum	76:83	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	0	59	theme	tetraploid	58:67	arg1	wheat					69:73	tetraploid wheat	58:73	tetraploid wheat (Triticum turgidum L.)	58:96	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	7	60	theme	branch	957:962	arg1	length					970:975	the highest branch chain length	945:975	the highest branch chain length of DP ≥ 37	945:986	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	0	61	theme	properties	44:53	arg1	Analysis					0:7	Analysis	0:7	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.	0:137	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	4	62	theme	starch	512:517	arg1	properties					498:507	the structural and functional properties	468:507	the structural and functional properties of starch	468:517	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	7	63	theme	chain	964:968	arg1	length					970:975	the highest branch chain length	945:975	the highest branch chain length of DP ≥ 37	945:986	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	12	64	theme	starch	1371:1376	arg1	structure					1378:1386	starch structure	1371:1386	starch structure	1371:1386	CONCLUSION Waxy proteins make a substantial contribution to starch structure.
35445411	6	65	theme	free	852:855	arg1	Wx-ab					865:869	Wx-ab	865:869	Wx-ab	865:869	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	6	65	theme	free	852:855	arg1	mutant					857:862	the Wx free mutant	845:862	the Wx free mutant (Wx-ab)	845:870	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	16	66	from	Wx-AB	1638:1642	arg1	Wx-Ab					1665:1669	Wx-Ab	1665:1669	Wx-Ab	1665:1669	The Wx-AB in native starch and Wx-Ab in gelatinized starch can control obesity and diabetes by slow-digesting carbohydrates and high resistance to digestion.
35445411	16	66	from	Wx-AB	1638:1642	arg1	starch					1686:1691	gelatinized starch	1674:1691	gelatinized starch	1674:1691	The Wx-AB in native starch and Wx-Ab in gelatinized starch can control obesity and diabetes by slow-digesting carbohydrates and high resistance to digestion.
35445411	16	66	from	Wx-AB	1638:1642	arg1	starch					1654:1659	native starch	1647:1659	native starch	1647:1659	The Wx-AB in native starch and Wx-Ab in gelatinized starch can control obesity and diabetes by slow-digesting carbohydrates and high resistance to digestion.
35445411	13	67	theme	proteins	1404:1411	arg1	lack					1391:1394	A lack	1389:1394	A lack of waxy proteins	1389:1411	A lack of waxy proteins reduced the unit chains markedly compared with the control.
35445411	7	68	theme	lowest	877:882	arg1	length					897:902	The lowest branch chain length	873:902	The lowest branch chain length	873:902	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	8	69	contain	had	995:997	arg2	degree					1011:1016	the highest degree	999:1016	the highest degree of crystallinity	999:1033	Wx-ab had the highest degree of crystallinity.
35445411	8	69	contain	had	995:997	arg1	Wx-ab					989:993	Wx-ab	989:993	Wx-ab	989:993	Wx-ab had the highest degree of crystallinity.
35445411	8	70	theme	highest	1003:1009	arg1	degree					1011:1016	the highest degree	999:1016	the highest degree of crystallinity	999:1033	Wx-ab had the highest degree of crystallinity.
35445411	4	71	theme	study	419:423	arg1	objective					401:409	The objective	397:409	The objective of this study	397:423	The objective of this study was to explore the effect of Waxy genes on the structural and functional properties of starch, with the aim of improving food quality.
35445411	9	72	theme	crystallinity	1040:1052	arg1	trend					1054:1058	The crystallinity trend	1036:1058	The crystallinity trend	1036:1058	The crystallinity trend followed the order Wx-ab>Wx-Ab>Wx-aB>Wx-AB.
35445411	14	73	theme	smaller	1514:1520	arg1	chains					1533:1538	the smaller and longer chains	1510:1538	the smaller and longer chains of starch	1510:1548	Waxy proteins significantly affected the smaller and longer chains of starch.
35445411	7	74	theme	DP ≥ 37	980:986	arg1	length					970:975	the highest branch chain length	945:975	the highest branch chain length of DP ≥ 37	945:986	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	0	75	theme	wheat	69:73	arg1	structure					19:27	starch structure	12:27	starch structure	12:27	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	0	75	theme	wheat	69:73	arg1	properties					44:53	functional properties	33:53	functional properties	33:53	Analysis of starch structure and functional properties of tetraploid wheat (Triticum turgidum L.) with differing waxy protein composition.
35445411	15	76	theme	food	1618:1621	arg1	digestion					1623:1631	food digestion	1618:1631	food digestion	1618:1631	The lines with differing waxy composition had different effects on food digestion.
35445411	10	77	theme	starch	1136:1141	arg1	SDS					1144:1146	SDS	1144:1146	SDS	1144:1146	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	10	77	theme	starch	1136:1141	arg1	higher					1153:1158	higher	1153:1158	higher	1153:1158	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	10	77	theme	starch	1136:1141	arg1	starch					1136:1141	slowly digestible starch	1118:1141	slowly digestible starch (SDS)	1118:1147	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	10	77	theme	starch	1136:1141	arg1	amount					1108:1113	The amount	1104:1113	The amount of slowly digestible starch (SDS)	1104:1147	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	3	78	theme	urgent	323:328	arg1	need					330:333	an urgent need	320:333	an urgent need to develop modified foods that can ameliorate such illnesses	320:394	Consequently, there is an urgent need to develop modified foods that can ameliorate such illnesses.
35445411	6	79	theme	Wx	849:850	arg1	Wx-ab					865:869	Wx-ab	865:869	Wx-ab	865:869	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	6	79	theme	Wx	849:850	arg1	mutant					857:862	the Wx free mutant	845:862	the Wx free mutant (Wx-ab)	845:870	RESULTS The proportion of B-type granules was higher in the mutants than in the wild-type (Wx-AB), and there were significant changes in the starch granule size, number, and phenotype in the Wx free mutant (Wx-ab).
35445411	13	80	theme	unit	1425:1428	arg1	chains					1430:1435	the unit chains	1421:1435	the unit chains	1421:1435	A lack of waxy proteins reduced the unit chains markedly compared with the control.
35445411	7	81	theme	branch	884:889	arg1	length					897:902	The lowest branch chain length	873:902	The lowest branch chain length	873:902	The lowest branch chain length was observed in Wx-ab, whereas Wx-AB had the highest branch chain length of DP ≥ 37.
35445411	16	82	theme	slow-digesting	1729:1742	arg1	carbohydrates					1744:1756	slow-digesting carbohydrates	1729:1756	slow-digesting carbohydrates	1729:1756	The Wx-AB in native starch and Wx-Ab in gelatinized starch can control obesity and diabetes by slow-digesting carbohydrates and high resistance to digestion.
35445411	10	83	theme	native	1163:1168	arg1	starch					1200:1205	native, gelatinized, and retrograded starch	1163:1205	native, gelatinized, and retrograded starch	1163:1205	The amount of slowly digestible starch (SDS) was higher in native, gelatinized, and retrograded starch in the mutant.
35445411	16	84	theme	gelatinized	1674:1684	arg1	starch					1686:1691	gelatinized starch	1674:1691	gelatinized starch	1674:1691	The Wx-AB in native starch and Wx-Ab in gelatinized starch can control obesity and diabetes by slow-digesting carbohydrates and high resistance to digestion.
35962940	6	0	theme	BC	921:922	arg1	production					924:933	BC production	921:933	BC production	921:933	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	9	1	theme	dressing	1539:1546	arg1	biomaterial					1548:1558	a promising wound dressing biomaterial	1521:1558	a promising wound dressing biomaterial	1521:1558	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	6	2	theme	PVA	910:912	arg1	addition					898:905	The addition	894:905	The addition of PVA during BC production	894:933	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	3	3	theme	genipin-crosslinked	554:572	arg1	sericin					579:585	genipin-crosslinked silk sericin	554:585	genipin-crosslinked silk sericin (SS)	554:590	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	3	theme	genipin-crosslinked	554:572	arg1	SS					588:589	SS	588:589	SS	588:589	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	2	4	theme	infection	279:287	arg1	management					251:260	The management	247:260	The management of chronic wound infection	247:287	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	5	5	theme	@	877:877	arg1	scaffolds					883:891	BC-PVA@SSga scaffolds	871:891	BC-PVA@SSga scaffolds	871:891	Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC-PVA@SSga scaffolds.
35962940	5	6	dep	Fourier	764:770	arg1	transform					772:780	transform	772:780	transform infrared spectroscopy	772:802	Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC-PVA@SSga scaffolds.
35962940	5	7	theme	scaffolds	883:891	arg1	components					857:866	the components	853:866	the components of BC-PVA@SSga scaffolds	853:891	Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC-PVA@SSga scaffolds.
35962940	6	8	dep	120	1091:1093	arg1	to					1088:1089	to	1088:1089	to	1088:1089	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	1	9	theme	microbial	200:208	arg1	communities					218:228	infectious microbial complex communities	189:228	infectious microbial complex communities called biofilms	189:244	Chronic wounds are associated with infectious microbial complex communities called biofilms.
35962940	8	10	theme	NIH3T3	1358:1363	arg1	cells					1365:1369	HaCaT and NIH3T3 cells	1348:1369	cells	1365:1369	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	6	11	dep	27.4 nm	1097:1103	arg1	±					1095:1095	±	1095:1095	±	1095:1095	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	5	12	theme	intermolecular	817:830	arg1	interactions					832:843	the intermolecular interactions	813:843	the intermolecular interactions between the components of BC-PVA@SSga scaffolds	813:891	Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC-PVA@SSga scaffolds.
35962940	0	13	theme	Wound	139:143	arg1	Biofilm					145:151	Chronic Wound Biofilm	131:151	Chronic Wound Biofilm	131:151	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	2	14	theme	chronic	265:271	arg1	infection					279:287	chronic wound infection	265:287	chronic wound infection	265:287	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	6	15	dep	%	981:981	arg1	%					1004:1004	2.3% to 83.5% ± 2.9%	985:1004	53.5% ± 2.3% to 83.5% ± 2.9%	977:1004	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	4	16	theme	dressing	661:668	arg1	material					670:677	a wound dressing material	653:677	a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm	653:761	The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm.
35962940	4	16	theme	dressing	661:668	arg1	composite					627:635	The composite	623:635	The composite	623:635	The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm.
35962940	0	17	theme	alcohol	58:64	arg1	Sericin					78:84	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	Sericin	78:84	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	9	18	theme	developed	1432:1440	arg1	SSga					1449:1452	The developed BC-PVA@SSga	1428:1452	The developed BC-PVA@SSga	1428:1452	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	6	19	theme	%	997:997	arg1	%					1004:1004	2.3% to 83.5% ± 2.9%	985:1004	53.5% ± 2.3% to 83.5% ± 2.9%	977:1004	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	0	20	theme	-Based	66:71	arg1	Sericin					78:84	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	Sericin	78:84	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	1	21	theme	Chronic	154:160	arg1	wounds					162:167	Chronic wounds	154:167	Chronic wounds	154:167	Chronic wounds are associated with infectious microbial complex communities called biofilms.
35962940	4	22	theme	microbial	745:753	arg1	biofilm					755:761	microbial biofilm	745:761	microbial biofilm	745:761	The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm.
35962940	6	23	theme	2.3	985:987	arg1	%					988:988	%	988:988	%	988:988	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	8	24	theme	biocompatibility	1271:1286	arg1	analysis					1288:1295	In vitro biocompatibility analysis	1262:1295	In vitro biocompatibility analysis	1262:1295	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	0	25	theme	Azithromycin	90:101	arg1	System					111:116	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	System	111:116	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	6	26	theme	±	1030:1030	arg1	4.5 µm					1046:1051	2.3 ± 1.9 to 16.8 ± 4.5 µm	1026:1051	2.3 ± 1.9 to 16.8 ± 4.5 µm	1026:1051	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	26	theme	±	1030:1030	arg1	diameter					1064:1071	the fiber diameter	1054:1071	the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm	1054:1103	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	2	27	theme	antimicrobial	402:414	arg1	resistance					416:425	antimicrobial resistance	402:425	antimicrobial resistance in biofilms	402:437	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	4	28	dep	development	698:708	arg1	the					694:696	the	694:696	the	694:696	The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm.
35962940	8	29	theme	nontoxic	1310:1317	arg1	nature					1319:1324	the nontoxic nature	1306:1324	the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells	1306:1369	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	9	30	theme	mouse	1496:1500	arg1	model					1502:1506	the infected mouse model	1483:1506	the infected mouse model	1483:1506	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	6	31	theme	pore	1011:1014	arg1	size					1016:1019	the pore size	1007:1019	the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm,	1007:1104	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	31	theme	pore	1011:1014	arg1	%					981:981	53.5% ±	977:983	53.5% ± 2.3% to 83.5% ± 2.9%	977:1004	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	32	dep	16.8	1039:1042	arg1	to					1036:1037	to	1036:1037	to	1036:1037	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	3	33	theme	bacterial	470:478	arg1	cellulose/poly					480:493	bacterial cellulose/poly	470:493	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	33	theme	bacterial	470:478	arg1	alcohol					501:507	vinyl alcohol	495:507	vinyl alcohol	495:507	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	33	theme	bacterial	470:478	arg1	BC-PVA					511:516	BC-PVA	511:516	BC-PVA	511:516	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	0	34	theme	Bacterial	27:35	arg1	Sericin					78:84	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	Sericin	78:84	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	2	35	with	dressing	360:367	arg1	activity					386:393	antibiofilm activity	374:393	antibiofilm activity	374:393	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	3	36	dep	in	452:453	arg1	situ					455:458	situ	455:458	situ	455:458	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	8	37	theme	@	1335:1335	arg1	SSga					1336:1339	BC-PVA@SSga	1329:1339	BC-PVA@SSga toward HaCaT and NIH3T3 cells	1329:1369	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	6	38	dep	%	988:988	arg1	to					990:991	to	990:991	to	990:991	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	39	theme	fiber	1058:1062	arg1	4.5 µm					1046:1051	2.3 ± 1.9 to 16.8 ± 4.5 µm	1026:1051	2.3 ± 1.9 to 16.8 ± 4.5 µm	1026:1051	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	39	theme	fiber	1058:1062	arg1	diameter					1064:1071	the fiber diameter	1054:1071	the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm	1054:1103	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	2	40	theme	appropriate	334:344	arg1	dressing					360:367	an appropriate antimicrobial dressing	331:367	an appropriate antimicrobial dressing with antibiofilm activity	331:393	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	9	41	theme	wound	1533:1537	arg1	biomaterial					1548:1558	a promising wound dressing biomaterial	1521:1558	a promising wound dressing biomaterial	1521:1558	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	2	42	theme	antibiofilm	374:384	arg1	activity					386:393	antibiofilm activity	374:393	antibiofilm activity	374:393	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	5	43	theme	SSga	878:881	arg1	scaffolds					883:891	BC-PVA@SSga scaffolds	871:891	BC-PVA@SSga scaffolds	871:891	Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC-PVA@SSga scaffolds.
35962940	5	44	theme	BC-PVA	871:876	arg1	scaffolds					883:891	BC-PVA@SSga scaffolds	871:891	BC-PVA@SSga scaffolds	871:891	Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC-PVA@SSga scaffolds.
35962940	8	45	dep	confirms	1297:1304	arg1	whereas					1372:1378	whereas	1372:1378	whereas	1372:1378	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	5	46	dep	transform	772:780	arg1	infrared					782:789	infrared	782:789	transform infrared spectroscopy	772:802	Fourier transform infrared spectroscopy confirms the intermolecular interactions between the components of BC-PVA@SSga scaffolds.
35962940	6	47	theme	±	1083:1083	arg1	27.4 nm					1097:1103	35.5 ± 10 to 120 ± 27.4 nm	1078:1103	35.5 ± 10 to 120 ± 27.4 nm	1078:1103	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	3	48	theme	silk	574:577	arg1	sericin					579:585	genipin-crosslinked silk sericin	554:585	genipin-crosslinked silk sericin (SS)	554:590	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	48	theme	silk	574:577	arg1	SS					588:589	SS	588:589	SS	588:589	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	1	49	theme	infectious	189:198	arg1	communities					218:228	infectious microbial complex communities	189:228	infectious microbial complex communities called biofilms	189:244	Chronic wounds are associated with infectious microbial complex communities called biofilms.
35962940	9	50	theme	@	1448:1448	arg1	SSga					1449:1452	The developed BC-PVA@SSga	1428:1452	The developed BC-PVA@SSga	1428:1452	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	7	51	theme	high	1200:1203	arg1	inhibition					1205:1214	high inhibition	1200:1214	high inhibition	1200:1214	Studies using bacteria and fungi indicate high inhibition and disruption of biofilms upon AZM addition.
35962940	0	52	theme	Chronic	131:137	arg1	Biofilm					145:151	Chronic Wound Biofilm	131:151	Chronic Wound Biofilm	131:151	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	1	53	theme	complex	210:216	arg1	communities					218:228	infectious microbial complex communities	189:228	infectious microbial complex communities called biofilms	189:244	Chronic wounds are associated with infectious microbial complex communities called biofilms.
35962940	6	54	dep	±	1095:1095	arg1	120					1091:1093	120	1091:1093	120	1091:1093	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	55	theme	thermal	1123:1129	arg1	stability					1131:1139	the thermal stability	1119:1139	the thermal stability	1119:1139	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	2	56	theme	wound	273:277	arg1	infection					279:287	chronic wound infection	265:287	chronic wound infection	265:287	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	8	57	theme	SS	1396:1397	arg1	addition					1384:1391	the addition	1380:1391	the addition of SS	1380:1397	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	8	58	theme	In	1262:1263	arg1	analysis					1288:1295	In vitro biocompatibility analysis	1262:1295	In vitro biocompatibility analysis	1262:1295	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	9	59	dep	accelerates	1454:1464	arg1	biomaterial					1548:1558	a promising wound dressing biomaterial	1521:1558	a promising wound dressing biomaterial	1521:1558	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	6	60	theme	±	999:999	arg1	%					1004:1004	2.3% to 83.5% ± 2.9%	985:1004	53.5% ± 2.3% to 83.5% ± 2.9%	977:1004	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	8	61	theme	cell	1408:1411	arg1	proliferation					1413:1425	cell proliferation	1408:1425	cell proliferation	1408:1425	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	4	62	theme	wound	655:659	arg1	material					670:677	a wound dressing material	653:677	a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm	653:761	The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm.
35962940	4	62	theme	wound	655:659	arg1	composite					627:635	The composite	623:635	The composite	623:635	The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm.
35962940	0	63	theme	Silk	73:76	arg1	Sericin					78:84	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	Sericin	78:84	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	9	64	theme	BC-PVA	1442:1447	arg1	SSga					1449:1452	The developed BC-PVA@SSga	1428:1452	The developed BC-PVA@SSga	1428:1452	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	6	65	theme	83.5	993:996	arg1	%					988:988	%	988:988	%	988:988	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	0	66	theme	Release	103:109	arg1	System					111:116	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	System	111:116	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	9	67	theme	wound	1466:1470	arg1	healing					1472:1478	wound healing	1466:1478	wound healing	1466:1478	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	7	68	theme	AZM	1248:1250	arg1	addition					1252:1259	AZM addition	1248:1259	AZM addition	1248:1259	Studies using bacteria and fungi indicate high inhibition and disruption of biofilms upon AZM addition.
35962940	3	69	link	genipin-crosslinked	554:572	arg1	sericin					579:585	genipin-crosslinked silk sericin	554:585	genipin-crosslinked silk sericin (SS)	554:590	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	69	link	genipin-crosslinked	554:572	arg1	SS					588:589	SS	588:589	SS	588:589	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	4	70	theme	biofilm	755:761	arg1	development					698:708	development	698:708	development	698:708	The composite is evaluated as a wound dressing material for preventing the development, dispersion, and/or eradication of microbial biofilm.
35962940	6	71	from	27.4 nm	1097:1103	arg1	4.5 µm					1046:1051	2.3 ± 1.9 to 16.8 ± 4.5 µm	1026:1051	2.3 ± 1.9 to 16.8 ± 4.5 µm	1026:1051	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	71	from	27.4 nm	1097:1103	arg1	diameter					1064:1071	the fiber diameter	1054:1071	the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm	1054:1103	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	3	72	theme	developed	460:468	arg1	composite					519:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	7	73	theme	biofilms	1234:1241	arg1	disruption					1220:1229	disruption	1220:1229	disruption of biofilms	1220:1241	Studies using bacteria and fungi indicate high inhibition and disruption of biofilms upon AZM addition.
35962940	7	73	theme	biofilms	1234:1241	arg1	inhibition					1205:1214	high inhibition	1200:1214	high inhibition	1200:1214	Studies using bacteria and fungi indicate high inhibition and disruption of biofilms upon AZM addition.
35962940	3	74	theme	in	452:453	arg1	composite					519:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	0	75	theme	Porous	20:25	arg1	Sericin					78:84	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	Sericin	78:84	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	9	76	theme	infected	1487:1494	arg1	model					1502:1506	the infected mouse model	1483:1506	the infected mouse model	1483:1506	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	3	77	theme	vinyl	495:499	arg1	cellulose/poly					480:493	bacterial cellulose/poly	470:493	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	77	theme	vinyl	495:499	arg1	alcohol					501:507	vinyl alcohol	495:507	vinyl alcohol	495:507	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	0	78	theme	Cellulose/Poly	37:50	arg1	Sericin					78:84	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	Sericin	78:84	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	8	79	dep	In	1262:1263	arg1	vitro					1265:1269	vitro	1265:1269	vitro	1265:1269	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	6	80	from	%	981:981	arg1	porosity					963:970	the porosity	959:970	the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm,	959:1104	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	3	81	theme	cellulose/poly	480:493	arg1	composite					519:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	0	82	theme	vinyl	52:56	arg1	Sericin					78:84	Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System	20:116	Sericin	78:84	In Situ Synthesized Porous Bacterial Cellulose/Poly(vinyl alcohol)-Based Silk Sericin and Azithromycin Release System for Treating Chronic Wound Biofilm.
35962940	8	83	theme	BC-PVA	1329:1334	arg1	SSga					1336:1339	BC-PVA@SSga	1329:1339	BC-PVA@SSga toward HaCaT and NIH3T3 cells	1329:1369	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	2	84	theme	antimicrobial	346:358	arg1	dressing					360:367	an appropriate antimicrobial dressing	331:367	an appropriate antimicrobial dressing with antibiofilm activity	331:393	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	3	85	mod	modified	540:547	arg1	composite					519:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite	448:527	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	85	mod	modified	540:547	arg3	SSga					616:619	SSga	616:619	SSga	616:619	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	85	mod	modified	540:547	arg3	AZM					610:612	AZM	610:612	AZM	610:612	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	85	mod	modified	540:547	arg3	SS					588:589	SS	588:589	SS	588:589	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	85	mod	modified	540:547	arg3	azithromycin					596:607	azithromycin	596:607	azithromycin (AZM) (SSga)	596:620	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	3	85	mod	modified	540:547	arg3	sericin					579:585	genipin-crosslinked silk sericin	554:585	genipin-crosslinked silk sericin (SS)	554:590	Herein, the in situ developed bacterial cellulose/poly(vinyl alcohol) (BC-PVA) composite is ex situ modified with genipin-crosslinked silk sericin (SS) and azithromycin (AZM) (SSga).
35962940	2	86	from	resistance	416:425	arg1	biofilms					430:437	biofilms	430:437	biofilms	430:437	The management of chronic wound infection is limited by the complexity of selecting an appropriate antimicrobial dressing with antibiofilm activity due to antimicrobial resistance in biofilms.
35962940	8	87	theme	SSga	1336:1339	arg1	nature					1319:1324	the nontoxic nature	1306:1324	the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells	1306:1369	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	9	88	theme	promising	1523:1531	arg1	biomaterial					1548:1558	a promising wound dressing biomaterial	1521:1558	a promising wound dressing biomaterial	1521:1558	The developed BC-PVA@SSga accelerates wound healing in the infected mouse model, thus can be a promising wound dressing biomaterial.
35962940	8	89	theme	HaCaT	1348:1352	arg1	cells					1365:1369	HaCaT and NIH3T3 cells	1348:1369	cells	1365:1369	In vitro biocompatibility analysis confirms the nontoxic nature of BC-PVA@SSga toward HaCaT and NIH3T3 cells, whereas the addition of SS enhances cell proliferation.
35962940	6	90	from	4.5 µm	1046:1051	arg1	size					1016:1019	the pore size	1007:1019	the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm,	1007:1104	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35962940	6	90	from	4.5 µm	1046:1051	arg1	%					981:981	53.5% ±	977:983	53.5% ± 2.3% to 83.5% ± 2.9%	977:1004	The addition of PVA during BC production significantly increases the porosity from 53.5% ± 2.3% to 83.5% ± 2.9%, the pore size from 2.3 ± 1.9 to 16.8 ± 4.5 µm, the fiber diameter from 35.5 ± 10 to 120 ± 27.4 nm, and improves the thermal stability and flexibility.
35199196	4	0	theme	normal	570:575	arg1	diet					577:580	a normal diet	568:580	a normal diet (ND, n = 5)	568:592	METHODS Male Wistar rats were fed with a normal diet (ND, n = 5) and a HFD (n = 20) for 24 weeks.
35199196	10	1	theme	HF-V	1224:1227	arg1	rats					1229:1232	ND-V and HF-V rats	1215:1232	ND-V and HF-V rats	1215:1232	The gut microbiota composition of ND-V and HF-V rats differed as indicated by beta diversity.
35199196	3	2	theme	HFD-induced	487:497	arg1	rats					523:526	HFD-induced obese-insulin-resistant rats	487:526	HFD-induced obese-insulin-resistant rats	487:526	Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats.
35199196	10	3	theme	ND-V	1215:1218	arg1	rats					1229:1232	ND-V and HF-V rats	1215:1232	ND-V and HF-V rats	1215:1232	The gut microbiota composition of ND-V and HF-V rats differed as indicated by beta diversity.
35199196	13	4	theme	HF-Pre	1436:1441	arg1	rats					1443:1446	HF-Pre rats	1436:1446	HF-Pre rats	1436:1446	HF-Pre rats showed a distinct gut microbiota the predominant family being Ruminococcaceae.
35199196	8	5	theme	HF-V	981:984	arg1	rats					986:989	HF-V rats	981:989	HF-V rats	981:989	RESULTS HF-V rats developed an obese-insulin-resistant condition as indicated by impaired metabolic parameters.
35199196	8	6	theme	metabolic	1063:1071	arg1	parameters					1073:1082	impaired metabolic parameters	1054:1082	impaired metabolic parameters	1054:1082	RESULTS HF-V rats developed an obese-insulin-resistant condition as indicated by impaired metabolic parameters.
35199196	5	7	dep	HF-Pro	697:702	arg1	paracasei					686:694	L. paracasei, HF-Pro, n = 5	683:709	paracasei	686:694	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	2	8	from	composition	339:349	arg1	rats					360:363	these rats	354:363	these rats	354:363	However, the gut microbiota composition in these rats has not been investigated.
35199196	1	9	theme	xylooligosaccharide	204:222	arg1	effects					138:144	the beneficial effects	123:144	the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats	123:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	8	10	theme	obese-insulin-resistant	1004:1026	arg1	condition					1028:1036	an obese-insulin-resistant condition	1001:1036	an obese-insulin-resistant condition	1001:1036	RESULTS HF-V rats developed an obese-insulin-resistant condition as indicated by impaired metabolic parameters.
35199196	1	11	theme	previous	93:100	arg1	studies					102:108	Our previous studies	89:108	Our previous studies	89:108	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	5	12	theme	HFD-fed	639:645	arg1	rats					647:650	HFD-fed rats	639:650	HFD-fed rats	639:650	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	15	13	with	treatment	1644:1652	arg1	prebiotic					1663:1671	prebiotic	1663:1671	prebiotic	1663:1671	The treatment with the prebiotic and synbiotic showed an association with the increase in Ruminococcaceae and Verrucomicrobia, respectively.
35199196	15	13	with	treatment	1644:1652	arg1	synbiotic					1677:1685	synbiotic	1677:1685	synbiotic	1677:1685	The treatment with the prebiotic and synbiotic showed an association with the increase in Ruminococcaceae and Verrucomicrobia, respectively.
35199196	1	14	theme	beneficial	127:136	arg1	effects					138:144	the beneficial effects	123:144	the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats	123:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	12	15	theme	HF-Syn	1423:1428	arg1	rats					1430:1433	the HF-Syn rats	1419:1433	the HF-Syn rats	1419:1433	Interestingly, Verrucomicrobia was also prominent in the HF-Syn rats.
35199196	5	16	dep	given	657:661	arg1	either					663:668	either	663:668	either	663:668	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	14	17	theme	HFD	1574:1576	arg1	consumption					1578:1588	HFD consumption	1574:1588	HFD consumption	1574:1588	CONCLUSION The changes in gut microbiota after HFD consumption included increased Firmicutes and Proteobacteria.
35199196	1	18	theme	probiotic	153:161	arg1	HII01					187:191	the probiotic Lactobacillus paracasei HII01	149:191	the probiotic Lactobacillus paracasei HII01	149:191	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	4	19	dep	METHODS	529:535	arg1	fed					559:561	fed	559:561	were fed with a normal diet (ND, n = 5) and a HFD (n = 20) for 24 weeks	554:624	METHODS Male Wistar rats were fed with a normal diet (ND, n = 5) and a HFD (n = 20) for 24 weeks.
35199196	6	20	theme	ND-fed	831:836	arg1	rats					838:841	ND-fed rats	831:841	ND-fed rats	831:841	ND-fed rats received vehicle (ND-V, n = 5).
35199196	0	21	from	Profiles	0:7	arg1	rats					54:57	obese-insulin-resistant rats	30:57	obese-insulin-resistant rats	30:57	Profiles of gut microbiota in obese-insulin-resistant rats treated with biotics.
35199196	10	22	theme	beta	1259:1262	arg1	diversity					1264:1272	beta diversity	1259:1272	beta diversity	1259:1272	The gut microbiota composition of ND-V and HF-V rats differed as indicated by beta diversity.
35199196	11	23	theme	HF-V	1341:1344	arg1	rats					1346:1349	HF-V rats	1341:1349	HF-V rats	1341:1349	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	16	24	theme	beneficial	1844:1853	arg1	effects					1855:1861	the beneficial effects	1840:1861	the beneficial effects on metabolic parameters	1840:1885	These changes in gut microbiota due to biotics may mediate the beneficial effects on metabolic parameters.
35199196	9	25	theme	same	1156:1159	arg1	level					1161:1165	the same level	1152:1165	the same level of ND-V rats	1152:1178	The prebiotic and synbiotic restored those metabolic parameters to the same level of ND-V rats.
35199196	1	26	from	effects	138:144	arg1	parameters					257:266	several parameters	249:266	several parameters in high-fat diet (HFD)-induced obese rats	249:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	1	27	dep	Lactobacillus	163:175	arg1	paracasei					177:185	paracasei	177:185	paracasei	177:185	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	11	28	from	Firmicutes	1308:1317	arg1	rats					1346:1349	HF-V rats	1341:1349	HF-V rats	1341:1349	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	11	28	from	Firmicutes	1308:1317	arg1	rats					1299:1302	ND-V rats	1294:1302	ND-V rats	1294:1302	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	5	29	dep	XOS + L.	756:763	arg1	paracasei					765:773	XOS + L. paracasei	756:773	XOS + L. paracasei	756:773	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	0	30	theme	gut	12:14	arg1	microbiota					16:25	gut microbiota	12:25	gut microbiota	12:25	Profiles of gut microbiota in obese-insulin-resistant rats treated with biotics.
35199196	9	31	theme	rats	1175:1178	arg1	level					1161:1165	the same level	1152:1165	the same level of ND-V rats	1152:1178	The prebiotic and synbiotic restored those metabolic parameters to the same level of ND-V rats.
35199196	11	32	from	Proteobacteria	1323:1336	arg1	rats					1346:1349	HF-V rats	1341:1349	HF-V rats	1341:1349	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	11	32	from	Proteobacteria	1323:1336	arg1	rats					1299:1302	ND-V rats	1294:1302	ND-V rats	1294:1302	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	5	33	dep	synbiotic	745:753	arg1	XOS + L.					756:763	XOS + L.	756:763	XOS + L.	756:763	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	5	33	dep	synbiotic	745:753	arg1	HF-Syn					776:781	HF-Syn	776:781	HF-Syn	776:781	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	1	34	theme	obese	299:303	arg1	rats					305:308	high-fat diet (HFD)-induced obese rats	271:308	high-fat diet (HFD)-induced obese rats	271:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	16	35	theme	gut	1798:1800	arg1	microbiota					1802:1811	gut microbiota	1798:1811	gut microbiota due to biotics	1798:1826	These changes in gut microbiota due to biotics may mediate the beneficial effects on metabolic parameters.
35199196	14	36	dep	CONCLUSION	1527:1536	arg1	included					1590:1597	included	1590:1597	included increased Firmicutes and Proteobacteria	1590:1637	CONCLUSION The changes in gut microbiota after HFD consumption included increased Firmicutes and Proteobacteria.
35199196	3	37	from	impact	439:444	arg1	microbiota					473:482	gut microbiota	469:482	gut microbiota in HFD-induced obese-insulin-resistant rats	469:526	Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats.
35199196	3	38	theme	gut	469:471	arg1	microbiota					473:482	gut microbiota	469:482	gut microbiota in HFD-induced obese-insulin-resistant rats	469:526	Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats.
35199196	7	39	theme	gut	943:945	arg1	microbiota					947:956	gut microbiota	943:956	gut microbiota	943:956	At week 24, all rats were decapitated, and metabolic parameters and gut microbiota were analyzed.
35199196	13	40	dep	showed	1448:1453	arg1	Ruminococcaceae					1510:1524	Ruminococcaceae	1510:1524	Ruminococcaceae	1510:1524	HF-Pre rats showed a distinct gut microbiota the predominant family being Ruminococcaceae.
35199196	13	40	dep	showed	1448:1453	arg1	family					1497:1502	the predominant family	1481:1502	the predominant family	1481:1502	HF-Pre rats showed a distinct gut microbiota the predominant family being Ruminococcaceae.
35199196	4	41	theme	Male	537:540	arg1	rats					549:552	Male Wistar rats	537:552	Male Wistar rats	537:552	METHODS Male Wistar rats were fed with a normal diet (ND, n = 5) and a HFD (n = 20) for 24 weeks.
35199196	13	42	theme	gut	1466:1468	arg1	microbiota					1470:1479	a distinct gut microbiota	1455:1479	a distinct gut microbiota	1455:1479	HF-Pre rats showed a distinct gut microbiota the predominant family being Ruminococcaceae.
35199196	15	43	from	increase	1718:1725	arg1	Verrucomicrobia					1750:1764	Verrucomicrobia	1750:1764	Verrucomicrobia	1750:1764	The treatment with the prebiotic and synbiotic showed an association with the increase in Ruminococcaceae and Verrucomicrobia, respectively.
35199196	15	43	from	increase	1718:1725	arg1	Ruminococcaceae					1730:1744	Ruminococcaceae	1730:1744	Ruminococcaceae	1730:1744	The treatment with the prebiotic and synbiotic showed an association with the increase in Ruminococcaceae and Verrucomicrobia, respectively.
35199196	5	44	theme	L.	683:684	arg1	n = 5					705:709	n = 5	705:709	n = 5	705:709	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	5	44	theme	L.	683:684	arg1	HF-Pro					697:702	HF-Pro	697:702	HF-Pro	697:702	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	3	45	theme	therapies	456:464	arg1	impact					439:444	the impact	435:444	the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats	435:526	Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats.
35199196	5	46	from	week	630:633	arg1	given					657:661	given	657:661	given	657:661	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	10	47	theme	microbiota	1189:1198	arg1	composition					1200:1210	The gut microbiota composition	1181:1210	The gut microbiota composition of ND-V and HF-V rats	1181:1232	The gut microbiota composition of ND-V and HF-V rats differed as indicated by beta diversity.
35199196	3	48	theme	obese-insulin-resistant	499:521	arg1	rats					523:526	HFD-induced obese-insulin-resistant rats	487:526	HFD-induced obese-insulin-resistant rats	487:526	Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats.
35199196	7	49	theme	metabolic	918:926	arg1	parameters					928:937	metabolic parameters	918:937	metabolic parameters	918:937	At week 24, all rats were decapitated, and metabolic parameters and gut microbiota were analyzed.
35199196	1	50	theme	high-fat	271:278	arg1	HFD					286:288	HFD	286:288	HFD	286:288	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	1	50	theme	high-fat	271:278	arg1	diet					280:283	high-fat diet	271:283	high-fat diet (HFD)	271:289	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	5	51	dep	prebiotic	713:721	arg1	n = 5					737:741	n = 5	737:741	n = 5	737:741	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	5	51	dep	prebiotic	713:721	arg1	HF-Pre					729:734	HF-Pre	729:734	HF-Pre	729:734	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	5	51	dep	prebiotic	713:721	arg1	XOS					724:726	XOS	724:726	XOS	724:726	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	2	52	theme	microbiota	328:337	arg1	composition					339:349	the gut microbiota composition	320:349	the gut microbiota composition in these rats	320:363	However, the gut microbiota composition in these rats has not been investigated.
35199196	1	53	theme	HII01	187:191	arg1	effects					138:144	the beneficial effects	123:144	the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats	123:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	8	54	theme	impaired	1054:1061	arg1	parameters					1073:1082	impaired metabolic parameters	1054:1082	impaired metabolic parameters	1054:1082	RESULTS HF-V rats developed an obese-insulin-resistant condition as indicated by impaired metabolic parameters.
35199196	1	55	theme	prebiotic	194:202	arg1	XOS					225:227	XOS	225:227	XOS	225:227	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	1	55	theme	prebiotic	194:202	arg1	xylooligosaccharide					204:222	prebiotic xylooligosaccharide	194:222	prebiotic xylooligosaccharide (XOS)	194:228	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	9	56	theme	metabolic	1128:1136	arg1	parameters					1138:1147	those metabolic parameters	1122:1147	those metabolic parameters	1122:1147	The prebiotic and synbiotic restored those metabolic parameters to the same level of ND-V rats.
35199196	3	57	from	microbiota	473:482	arg1	rats					523:526	HFD-induced obese-insulin-resistant rats	487:526	HFD-induced obese-insulin-resistant rats	487:526	Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats.
35199196	1	58	from	parameters	257:266	arg1	rats					305:308	high-fat diet (HFD)-induced obese rats	271:308	high-fat diet (HFD)-induced obese rats	271:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	14	59	theme	gut	1553:1555	arg1	microbiota					1557:1566	gut microbiota	1553:1566	gut microbiota	1553:1566	CONCLUSION The changes in gut microbiota after HFD consumption included increased Firmicutes and Proteobacteria.
35199196	5	60	dep	probiotic	672:680	arg1	n = 5					705:709	n = 5	705:709	n = 5	705:709	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	5	60	dep	probiotic	672:680	arg1	HF-Pro					697:702	HF-Pro	697:702	HF-Pro	697:702	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	10	61	theme	rats	1229:1232	arg1	composition					1200:1210	The gut microbiota composition	1181:1210	The gut microbiota composition of ND-V and HF-V rats	1181:1232	The gut microbiota composition of ND-V and HF-V rats differed as indicated by beta diversity.
35199196	16	62	from	effects	1855:1861	arg1	parameters					1876:1885	metabolic parameters	1866:1885	metabolic parameters	1866:1885	These changes in gut microbiota due to biotics may mediate the beneficial effects on metabolic parameters.
35199196	1	63	dep	demonstrated	110:121	arg1	PURPOSE					81:87	PURPOSE	81:87	PURPOSE	81:87	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	12	64	from	prominent	1406:1414	arg1	rats					1430:1433	the HF-Syn rats	1419:1433	the HF-Syn rats	1419:1433	Interestingly, Verrucomicrobia was also prominent in the HF-Syn rats.
35199196	1	65	theme	Lactobacillus	163:175	arg1	HII01					187:191	the probiotic Lactobacillus paracasei HII01	149:191	the probiotic Lactobacillus paracasei HII01	149:191	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	14	66	from	changes	1542:1548	arg1	microbiota					1557:1566	gut microbiota	1553:1566	gut microbiota	1553:1566	CONCLUSION The changes in gut microbiota after HFD consumption included increased Firmicutes and Proteobacteria.
35199196	8	67	dep	RESULTS	973:979	arg1	developed					991:999	developed	991:999	developed an obese-insulin-resistant condition as indicated by impaired metabolic parameters	991:1082	RESULTS HF-V rats developed an obese-insulin-resistant condition as indicated by impaired metabolic parameters.
35199196	5	68	dep	vehicle	795:801	arg1	n = 5					810:814	n = 5	810:814	n = 5	810:814	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	5	68	dep	vehicle	795:801	arg1	HF-V					804:807	HF-V	804:807	HF-V	804:807	At week 13, HFD-fed rats were given either a probiotic (L. paracasei, HF-Pro, n = 5), prebiotic (XOS, HF-Pre, n = 5), synbiotic (XOS + L. paracasei, HF-Syn, n = 5), or vehicle (HF-V, n = 5) for 12 weeks.
35199196	6	69	dep	vehicle	852:858	arg1	n = 5					867:871	n = 5	867:871	n = 5	867:871	ND-fed rats received vehicle (ND-V, n = 5).
35199196	6	69	dep	vehicle	852:858	arg1	ND-V					861:864	ND-V	861:864	ND-V	861:864	ND-fed rats received vehicle (ND-V, n = 5).
35199196	16	70	theme	metabolic	1866:1874	arg1	parameters					1876:1885	metabolic parameters	1866:1885	metabolic parameters	1866:1885	These changes in gut microbiota due to biotics may mediate the beneficial effects on metabolic parameters.
35199196	11	71	theme	ND-V	1294:1297	arg1	rats					1299:1302	ND-V rats	1294:1302	ND-V rats	1294:1302	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	4	72	dep	diet	577:580	arg1	n = 5					587:591	n = 5	587:591	n = 5	587:591	METHODS Male Wistar rats were fed with a normal diet (ND, n = 5) and a HFD (n = 20) for 24 weeks.
35199196	4	72	dep	diet	577:580	arg1	ND					583:584	ND	583:584	ND	583:584	METHODS Male Wistar rats were fed with a normal diet (ND, n = 5) and a HFD (n = 20) for 24 weeks.
35199196	0	73	theme	microbiota	16:25	arg1	Profiles					0:7	Profiles	0:7	Profiles of gut microbiota in obese-insulin-resistant rats	0:57	Profiles of gut microbiota in obese-insulin-resistant rats treated with biotics.
35199196	9	74	theme	ND-V	1170:1173	arg1	rats					1175:1178	ND-V rats	1170:1178	ND-V rats	1170:1178	The prebiotic and synbiotic restored those metabolic parameters to the same level of ND-V rats.
35199196	16	75	from	changes	1787:1793	arg1	microbiota					1802:1811	gut microbiota	1798:1811	gut microbiota due to biotics	1798:1826	These changes in gut microbiota due to biotics may mediate the beneficial effects on metabolic parameters.
35199196	13	76	theme	predominant	1485:1495	arg1	Ruminococcaceae					1510:1524	Ruminococcaceae	1510:1524	Ruminococcaceae	1510:1524	HF-Pre rats showed a distinct gut microbiota the predominant family being Ruminococcaceae.
35199196	13	76	theme	predominant	1485:1495	arg1	family					1497:1502	the predominant family	1481:1502	the predominant family	1481:1502	HF-Pre rats showed a distinct gut microbiota the predominant family being Ruminococcaceae.
35199196	3	77	theme	biotic	449:454	arg1	therapies					456:464	biotic therapies	449:464	biotic therapies	449:464	Therefore, this study aimed to investigate the impact of biotic therapies on gut microbiota in HFD-induced obese-insulin-resistant rats.
35199196	0	78	theme	obese-insulin-resistant	30:52	arg1	rats					54:57	obese-insulin-resistant rats	30:57	obese-insulin-resistant rats	30:57	Profiles of gut microbiota in obese-insulin-resistant rats treated with biotics.
35199196	1	79	theme	-induced	290:297	arg1	rats					305:308	high-fat diet (HFD)-induced obese rats	271:308	high-fat diet (HFD)-induced obese rats	271:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	11	80	from	Verrucomicrobia	1275:1289	arg1	rats					1346:1349	HF-V rats	1341:1349	HF-V rats	1341:1349	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	11	80	from	Verrucomicrobia	1275:1289	arg1	rats					1299:1302	ND-V rats	1294:1302	ND-V rats	1294:1302	Verrucomicrobia in ND-V rats and Firmicutes and Proteobacteria in HF-V rats were dominant.
35199196	12	81	from	rats	1430:1433	arg1	prominent					1406:1414	prominent	1406:1414	prominent	1406:1414	Interestingly, Verrucomicrobia was also prominent in the HF-Syn rats.
35199196	4	82	theme	Wistar	542:547	arg1	rats					549:552	Male Wistar rats	537:552	Male Wistar rats	537:552	METHODS Male Wistar rats were fed with a normal diet (ND, n = 5) and a HFD (n = 20) for 24 weeks.
35199196	13	83	theme	distinct	1457:1464	arg1	microbiota					1470:1479	a distinct gut microbiota	1455:1479	a distinct gut microbiota	1455:1479	HF-Pre rats showed a distinct gut microbiota the predominant family being Ruminococcaceae.
35199196	2	84	theme	gut	324:326	arg1	composition					339:349	the gut microbiota composition	320:349	the gut microbiota composition in these rats	320:363	However, the gut microbiota composition in these rats has not been investigated.
35199196	1	85	theme	synbiotics	235:244	arg1	effects					138:144	the beneficial effects	123:144	the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats	123:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	1	86	theme	several	249:255	arg1	parameters					257:266	several parameters	249:266	several parameters in high-fat diet (HFD)-induced obese rats	249:308	PURPOSE Our previous studies demonstrated the beneficial effects of the probiotic Lactobacillus paracasei HII01, prebiotic xylooligosaccharide (XOS), and synbiotics on several parameters in high-fat diet (HFD)-induced obese rats.
35199196	10	87	theme	gut	1185:1187	arg1	composition					1200:1210	The gut microbiota composition	1181:1210	The gut microbiota composition of ND-V and HF-V rats	1181:1232	The gut microbiota composition of ND-V and HF-V rats differed as indicated by beta diversity.
35199196	14	88	theme	increased	1599:1607	arg1	Firmicutes					1609:1618	Firmicutes	1609:1618	Firmicutes	1609:1618	CONCLUSION The changes in gut microbiota after HFD consumption included increased Firmicutes and Proteobacteria.
36206869	11	0	theme	species	2513:2519	arg1	identification					2521:2534	species identification	2513:2534	species identification	2513:2534	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	10	1	theme	anti-inflammatory	2154:2170	arg1	effects					2193:2199	their anti-inflammatory and antihyperalgesic effects	2148:2199	their anti-inflammatory and antihyperalgesic effects	2148:2199	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	3	2	theme	METHODS	721:727	arg1	parts					736:740	MATERIALS AND METHODS Aerial parts	707:740	MATERIALS AND METHODS Aerial parts of S. terebinthifolia	707:762	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	6	3	theme	%	1507:1507	arg1	sesquiterpenes					1487:1500	oxygenated sesquiterpenes	1476:1500	oxygenated sesquiterpenes (4.87%-14.38%)	1476:1515	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	3	theme	%	1507:1507	arg1	%					1514:1514	4.87%-14.38%	1503:1514	4.87%-14.38%	1503:1514	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	4	4	dep	histochemistry	927:940	arg1	The					923:925	The	923:925	The	923:925	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	0	5	theme	Raddi	163:167	arg1	histochemistry					121:134	histochemistry	121:134	histochemistry	121:134	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	0	5	theme	Raddi	163:167	arg1	micromorphology					101:115	micromorphology	101:115	micromorphology	101:115	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	0	5	theme	Raddi	163:167	arg1	variation					13:21	Geographical variation	0:21	Geographical variation in the chemical composition	0:49	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	8	6	theme	comprehensive	1692:1704	arg1	description					1706:1716	A comprehensive description	1690:1716	A comprehensive description of the leaf and stem micromorphology and histochemistry	1690:1772	A comprehensive description of the leaf and stem micromorphology and histochemistry was performed.
36206869	9	7	theme	carrageenan-induced	1903:1921	arg1	hyperalgesia					1964:1975	mechanical and thermal hyperalgesia	1941:1975	mechanical and thermal hyperalgesia	1941:1975	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	9	7	theme	carrageenan-induced	1903:1921	arg1	inflammation					1927:1938	carrageenan-induced paw inflammation	1903:1938	a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg	1901:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	1	8	theme	inflammations	407:419	arg1	treatment					394:402	the treatment	390:402	the treatment of inflammations	390:419	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	6	9	theme	oxygenated	1476:1485	arg1	sesquiterpenes					1487:1500	oxygenated sesquiterpenes	1476:1500	oxygenated sesquiterpenes (4.87%-14.38%)	1476:1515	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	9	theme	oxygenated	1476:1485	arg1	%					1514:1514	4.87%-14.38%	1503:1514	4.87%-14.38%	1503:1514	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	8	10	dep	leaf	1725:1728	arg1	the					1721:1723	the	1721:1723	the	1721:1723	A comprehensive description of the leaf and stem micromorphology and histochemistry was performed.
36206869	9	11	theme	inflammation	1927:1938	arg1	model					1978:1982	a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model	1901:1982	a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg	1901:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	6	12	dep	RESULTS	1247:1253	arg1	showed					1264:1269	showed	1264:1269	showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%)	1264:1515	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	8	13	theme	leaf	1725:1728	arg1	description					1706:1716	A comprehensive description	1690:1716	A comprehensive description of the leaf and stem micromorphology and histochemistry	1690:1772	A comprehensive description of the leaf and stem micromorphology and histochemistry was performed.
36206869	9	14	theme	mechanical	1941:1950	arg1	hyperalgesia					1964:1975	mechanical and thermal hyperalgesia	1941:1975	mechanical and thermal hyperalgesia	1941:1975	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	9	14	theme	mechanical	1941:1950	arg1	inflammation					1927:1938	carrageenan-induced paw inflammation	1903:1938	a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg	1901:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	6	15	theme	chemical	1334:1341	arg1	composition					1343:1353	chemical composition	1334:1353	chemical composition	1334:1353	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	8	16	theme	stem	1734:1737	arg1	micromorphology					1739:1753	stem micromorphology	1734:1753	stem micromorphology	1734:1753	A comprehensive description of the leaf and stem micromorphology and histochemistry was performed.
36206869	10	17	dep	effect	2244:2249	arg1	collaborating					2270:2282	collaborating	2270:2282	collaborating	2270:2282	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	1	18	used	used	357:360	arg2	it					341:342	it	341:342	it	341:342	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	1	18	used	used	357:360	arg2	Anacardiaceae					232:244	Anacardiaceae	232:244	Anacardiaceae	232:244	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	1	18	used	used	357:360	arg2	Raddi					225:229	Raddi	225:229	Raddi	225:229	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	10	19	theme	anti-inflammatory	2341:2357	arg1	effect					2359:2364	an anti-inflammatory effect	2338:2364	its an anti-inflammatory effect	2334:2364	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	2	20	theme	stems	700:704	arg1	histochemistry					651:664	histochemistry	651:664	histochemistry	651:664	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	2	20	theme	stems	700:704	arg1	micromorphology					670:684	micromorphology	670:684	micromorphology	670:684	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	9	21	with	model	1978:1982	arg1	doses					1994:1998	oral doses	1989:1998	oral doses of 30 mg/kg	1989:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	9	22	theme	thermal	1956:1962	arg1	hyperalgesia					1964:1975	mechanical and thermal hyperalgesia	1941:1975	mechanical and thermal hyperalgesia	1941:1975	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	9	22	theme	thermal	1956:1962	arg1	inflammation					1927:1938	carrageenan-induced paw inflammation	1903:1938	a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg	1901:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	7	23	theme	predominant	1550:1560	arg1	constituents					1562:1573	predominant constituents	1550:1573	predominant constituents of essential	1550:1586	Both α-pinene and limonene were predominant constituents of essential in five regions, except for one state where α-phellandrene and limonene were the dominant components.
36206869	3	24	theme	Brazil	796:801	arg1	states					786:791	six states	782:791	six states of Brazil	782:801	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	5	25	theme	anti-inflammatory	1150:1166	arg1	activity					1168:1175	anti-inflammatory activity	1150:1175	anti-inflammatory activity	1150:1175	The EOST were evaluated for anti-inflammatory activity and hyperalgesia using the carrageenan-induced paw edema methodology.
36206869	4	26	theme	scanning	1047:1054	arg1	microscopy					1065:1074	field emission scanning electron microscopy	1032:1074	field emission scanning electron microscopy	1032:1074	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	26	theme	scanning	1047:1054	arg1	reagents					1012:1019	standard reagents	1003:1019	standard reagents	1003:1019	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	3	27	theme	chromatography-mass	881:899	arg1	GC-MS					915:919	GC-MS	915:919	GC-MS	915:919	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	3	27	theme	chromatography-mass	881:899	arg1	spectrometry					901:912	gas chromatography-mass spectrometry	877:912	gas chromatography-mass spectrometry (GC-MS)	877:920	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	2	28	theme	essential	509:517	arg1	oil					519:521	the essential oil	505:521	the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil	505:584	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	10	29	theme	antihyperalgesic	2176:2191	arg1	effects					2193:2199	their anti-inflammatory and antihyperalgesic effects	2148:2199	their anti-inflammatory and antihyperalgesic effects	2148:2199	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	1	30	theme	traditional	365:375	arg1	medicine					377:384	traditional medicine	365:384	traditional medicine	365:384	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	4	31	theme	leaves	965:970	arg1	histochemistry					927:940	histochemistry	927:940	histochemistry	927:940	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	31	theme	leaves	965:970	arg1	micromorphology					946:960	micromorphology	946:960	micromorphology	946:960	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	3	32	theme	essential	812:820	arg1	oil					822:824	the essential oil	808:824	the essential oil	808:824	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	0	33	theme	oil	96:98	arg1	activity					70:77	anti-inflammatory activity	52:77	anti-inflammatory activity of the essential oil	52:98	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	0	33	theme	oil	96:98	arg1	variation					13:21	Geographical variation	0:21	Geographical variation in the chemical composition	0:49	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	2	34	theme	terebinthifolia	529:543	arg1	oil					519:521	the essential oil	505:521	the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil	505:584	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	9	35	theme	30 mg/kg	2003:2010	arg1	doses					1994:1998	oral doses	1989:1998	oral doses of 30 mg/kg	1989:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	6	36	theme	28.76	1382:1386	arg1	%					1394:1394	28.76%-47.73%	1382:1394	28.76%-47.73%	1382:1394	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	36	theme	28.76	1382:1386	arg1	monoterpenes					1368:1379	hydrocarbon monoterpenes	1356:1379	hydrocarbon monoterpenes (28.76%-47.73%)	1356:1395	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	4	37	theme	emission	1038:1045	arg1	microscopy					1065:1074	field emission scanning electron microscopy	1032:1074	field emission scanning electron microscopy	1032:1074	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	37	theme	emission	1038:1045	arg1	reagents					1012:1019	standard reagents	1003:1019	standard reagents	1003:1019	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	1	38	theme	sesquiterpenes	325:338	arg1	predominance					292:303	a predominance	290:303	a predominance of monoterpenes and sesquiterpenes	290:338	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	2	39	theme	leaves	689:694	arg1	histochemistry					651:664	histochemistry	651:664	histochemistry	651:664	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	2	39	theme	leaves	689:694	arg1	micromorphology					670:684	micromorphology	670:684	micromorphology	670:684	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	0	40	theme	Geographical	0:11	arg1	histochemistry					121:134	histochemistry	121:134	histochemistry	121:134	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	0	40	theme	Geographical	0:11	arg1	micromorphology					101:115	micromorphology	101:115	micromorphology	101:115	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	0	40	theme	Geographical	0:11	arg1	variation					13:21	Geographical variation	0:21	Geographical variation in the chemical composition	0:49	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	0	40	theme	Geographical	0:11	arg1	activity					70:77	anti-inflammatory activity	52:77	anti-inflammatory activity of the essential oil	52:98	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	5	41	theme	edema	1228:1232	arg1	methodology					1234:1244	the carrageenan-induced paw edema methodology	1200:1244	the carrageenan-induced paw edema methodology	1200:1244	The EOST were evaluated for anti-inflammatory activity and hyperalgesia using the carrageenan-induced paw edema methodology.
36206869	4	42	theme	electron	1056:1063	arg1	microscopy					1065:1074	field emission scanning electron microscopy	1032:1074	field emission scanning electron microscopy	1032:1074	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	42	theme	electron	1056:1063	arg1	reagents					1012:1019	standard reagents	1003:1019	standard reagents	1003:1019	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	10	43	theme	synergistic	2232:2242	arg1	effect					2244:2249	the synergistic effect	2228:2249	the synergistic effect	2228:2249	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	11	44	dep	provide	2452:2458	arg1	aids					2508:2511	aids	2508:2511	aids	2508:2511	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	6	45	from	sesquiterpenes	1487:1500	arg1	yield					1310:1314	its yield	1306:1314	its yield (0.40%-0.86%)	1306:1328	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	45	from	sesquiterpenes	1487:1500	arg1	composition					1343:1353	chemical composition	1334:1353	chemical composition	1334:1353	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	45	from	sesquiterpenes	1487:1500	arg1	%					1327:1327	0.40%-0.86%	1317:1327	0.40%-0.86%	1317:1327	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	9	46	theme	in	1796:1797	arg1	testing					1804:1810	the in vivo testing	1792:1810	the in vivo testing	1792:1810	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	0	47	theme	chemical	30:37	arg1	composition					39:49	the chemical composition	26:49	the chemical composition	26:49	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	6	48	theme	%	1420:1420	arg1	monoterpenes					1368:1379	hydrocarbon monoterpenes	1356:1379	hydrocarbon monoterpenes (28.76%-47.73%)	1356:1395	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	48	theme	%	1420:1420	arg1	%					1427:1427	31.43%-41.76%	1415:1427	31.43%-41.76%	1415:1427	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	2	49	theme	chemical	481:488	arg1	composition					490:500	the chemical composition	477:500	the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil	477:584	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	5	50	theme	carrageenan-induced	1204:1222	arg1	methodology					1234:1244	the carrageenan-induced paw edema methodology	1200:1244	the carrageenan-induced paw edema methodology	1200:1244	The EOST were evaluated for anti-inflammatory activity and hyperalgesia using the carrageenan-induced paw edema methodology.
36206869	10	51	theme	EOST	2054:2057	arg1	samples					2059:2065	the EOST samples	2050:2065	the EOST samples collected in six Brazilian states	2050:2099	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	6	52	from	monoterpenes	1442:1453	arg1	yield					1310:1314	its yield	1306:1314	its yield (0.40%-0.86%)	1306:1328	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	52	from	monoterpenes	1442:1453	arg1	composition					1343:1353	chemical composition	1334:1353	chemical composition	1334:1353	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	52	from	monoterpenes	1442:1453	arg1	%					1327:1327	0.40%-0.86%	1317:1327	0.40%-0.86%	1317:1327	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	0	53	theme	anti-inflammatory	52:68	arg1	activity					70:77	anti-inflammatory activity	52:77	anti-inflammatory activity of the essential oil	52:98	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	0	53	theme	anti-inflammatory	52:68	arg1	variation					13:21	Geographical variation	0:21	Geographical variation in the chemical composition	0:49	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	9	54	theme	antiedematogenic	1838:1853	arg1	effects					1877:1883	antiedematogenic and anti-hyperalgesic effects	1838:1883	antiedematogenic and anti-hyperalgesic effects	1838:1883	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	4	55	theme	standard	1003:1010	arg1	light					1022:1026	light	1022:1026	light	1022:1026	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	55	theme	standard	1003:1010	arg1	reagents					1012:1019	standard reagents	1003:1019	standard reagents	1003:1019	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	55	theme	standard	1003:1010	arg1	microscopy					1065:1074	field emission scanning electron microscopy	1032:1074	field emission scanning electron microscopy	1032:1074	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	2	56	theme	anti-inflammatory	600:616	arg1	effects					618:624	its anti-inflammatory effects	596:624	its anti-inflammatory effects in mice	596:632	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	4	57	theme	energy-dispersive	1084:1100	arg1	spectroscopy					1108:1119	energy-dispersive X-ray spectroscopy	1084:1119	energy-dispersive X-ray spectroscopy	1084:1119	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	6	58	theme	%	1321:1321	arg1	yield					1310:1314	its yield	1306:1314	its yield (0.40%-0.86%)	1306:1328	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	58	theme	%	1321:1321	arg1	%					1327:1327	0.40%-0.86%	1317:1327	0.40%-0.86%	1317:1327	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	10	59	theme	components	2258:2267	arg1	effect					2244:2249	the synergistic effect	2228:2249	the synergistic effect	2228:2249	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	10	60	theme	Brazilian	2084:2092	arg1	states					2094:2099	six Brazilian states	2080:2099	six Brazilian states	2080:2099	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	3	61	theme	Aerial	729:734	arg1	parts					736:740	MATERIALS AND METHODS Aerial parts	707:740	MATERIALS AND METHODS Aerial parts of S. terebinthifolia	707:762	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	9	62	dep	in	1796:1797	arg1	vivo					1799:1802	vivo	1799:1802	vivo	1799:1802	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	11	63	theme	microchemical	2475:2487	arg1	information					2489:2499	anatomical and microchemical information	2460:2499	anatomical and microchemical information	2460:2499	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	10	64	theme	etnhopharmacologycal	2288:2307	arg1	use					2309:2311	the etnhopharmacologycal use	2284:2311	the etnhopharmacologycal use of this plant	2284:2325	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	10	65	dep	CONCLUSION	2013:2022	arg1	indicate					2036:2043	indicate	2036:2043	indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect	2036:2364	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	9	66	theme	paw	1923:1925	arg1	hyperalgesia					1964:1975	mechanical and thermal hyperalgesia	1941:1975	mechanical and thermal hyperalgesia	1941:1975	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	9	66	theme	paw	1923:1925	arg1	inflammation					1927:1938	carrageenan-induced paw inflammation	1903:1938	a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg	1901:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	3	67	theme	terebinthifolia	748:762	arg1	parts					736:740	MATERIALS AND METHODS Aerial parts	707:740	MATERIALS AND METHODS Aerial parts of S. terebinthifolia	707:762	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	6	68	theme	hydrocarbon	1356:1366	arg1	sesquiterpenes					1398:1411	sesquiterpenes	1398:1411	sesquiterpenes	1398:1411	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	68	theme	hydrocarbon	1356:1366	arg1	%					1394:1394	28.76%-47.73%	1382:1394	28.76%-47.73%	1382:1394	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	68	theme	hydrocarbon	1356:1366	arg1	monoterpenes					1368:1379	hydrocarbon monoterpenes	1356:1379	hydrocarbon monoterpenes (28.76%-47.73%)	1356:1395	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	68	theme	hydrocarbon	1356:1366	arg1	%					1427:1427	31.43%-41.76%	1415:1427	31.43%-41.76%	1415:1427	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	7	69	theme	essential	1578:1586	arg1	constituents					1562:1573	predominant constituents	1550:1573	predominant constituents of essential	1550:1586	Both α-pinene and limonene were predominant constituents of essential in five regions, except for one state where α-phellandrene and limonene were the dominant components.
36206869	10	70	theme	plant	2321:2325	arg1	use					2309:2311	the etnhopharmacologycal use	2284:2311	the etnhopharmacologycal use of this plant	2284:2325	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	1	71	from	oil	268:270	arg1	rich					250:253	rich	250:253	rich	250:253	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	1	72	from	rich	250:253	arg1	oil					268:270	essential oil	258:270	essential oil	258:270	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	2	73	theme	Brazil	579:584	arg1	states					569:574	six states	565:574	six states of Brazil	565:584	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	11	74	theme	stems	2422:2426	arg1	micromorphology					2373:2387	micromorphology	2373:2387	micromorphology	2373:2387	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	11	74	theme	stems	2422:2426	arg1	histochemistry					2393:2406	histochemistry	2393:2406	histochemistry	2393:2406	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	11	75	theme	anatomical	2460:2469	arg1	information					2489:2499	anatomical and microchemical information	2460:2499	anatomical and microchemical information	2460:2499	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	6	76	from	states	1296:1301	arg1	yield					1310:1314	its yield	1306:1314	its yield (0.40%-0.86%)	1306:1328	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	76	from	states	1296:1301	arg1	composition					1343:1353	chemical composition	1334:1353	chemical composition	1334:1353	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	76	from	states	1296:1301	arg1	%					1327:1327	0.40%-0.86%	1317:1327	0.40%-0.86%	1317:1327	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	7	77	theme	dominant	1669:1676	arg1	components					1678:1687	the dominant components	1665:1687	the dominant components	1665:1687	Both α-pinene and limonene were predominant constituents of essential in five regions, except for one state where α-phellandrene and limonene were the dominant components.
36206869	7	77	theme	dominant	1669:1676	arg1	limonene					1651:1658	limonene	1651:1658	limonene	1651:1658	Both α-pinene and limonene were predominant constituents of essential in five regions, except for one state where α-phellandrene and limonene were the dominant components.
36206869	7	77	theme	dominant	1669:1676	arg1	α-phellandrene					1632:1645	α-phellandrene	1632:1645	α-phellandrene	1632:1645	Both α-pinene and limonene were predominant constituents of essential in five regions, except for one state where α-phellandrene and limonene were the dominant components.
36206869	8	78	theme	micromorphology	1739:1753	arg1	description					1706:1716	A comprehensive description	1690:1716	A comprehensive description of the leaf and stem micromorphology and histochemistry	1690:1772	A comprehensive description of the leaf and stem micromorphology and histochemistry was performed.
36206869	9	79	theme	EOST	1817:1820	arg1	samples					1822:1828	all EOST samples	1813:1828	all EOST samples	1813:1828	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	2	80	theme	oil	519:521	arg1	composition					490:500	the chemical composition	477:500	the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil	477:584	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	0	81	theme	essential	86:94	arg1	oil					96:98	the essential oil	82:98	the essential oil	82:98	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	7	82	from	constituents	1562:1573	arg1	regions					1596:1602	five regions	1591:1602	five regions	1591:1602	Both α-pinene and limonene were predominant constituents of essential in five regions, except for one state where α-phellandrene and limonene were the dominant components.
36206869	0	83	from	variation	13:21	arg1	composition					39:49	the chemical composition	26:49	the chemical composition	26:49	Geographical variation in the chemical composition, anti-inflammatory activity of the essential oil, micromorphology and histochemistry of Schinus terebinthifolia Raddi.
36206869	9	84	theme	oral	1989:1992	arg1	doses					1994:1998	oral doses	1989:1998	oral doses of 30 mg/kg	1989:2010	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	2	85	dep	histochemistry	651:664	arg1	the					647:649	the	647:649	the	647:649	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	10	86	theme	chemical	2119:2126	arg1	composition					2128:2138	their chemical composition	2113:2138	their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect	2113:2364	CONCLUSION Our results indicate that the EOST samples collected in six Brazilian states differed in their chemical composition but not their anti-inflammatory and antihyperalgesic effects, which was correlated with the synergistic effect of its components, collaborating the etnhopharmacologycal use of this plant due to its an anti-inflammatory effect.
36206869	6	87	theme	%	1387:1387	arg1	%					1394:1394	28.76%-47.73%	1382:1394	28.76%-47.73%	1382:1394	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	87	theme	%	1387:1387	arg1	monoterpenes					1368:1379	hydrocarbon monoterpenes	1356:1379	hydrocarbon monoterpenes (28.76%-47.73%)	1356:1395	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	11	88	theme	leaves	2411:2416	arg1	micromorphology					2373:2387	micromorphology	2373:2387	micromorphology	2373:2387	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	11	88	theme	leaves	2411:2416	arg1	histochemistry					2393:2406	histochemistry	2393:2406	histochemistry	2393:2406	Also, micromorphology and histochemistry of leaves and stems presented in this study provide anatomical and microchemical information, which aids species identification.
36206869	4	89	theme	stems	976:980	arg1	histochemistry					927:940	histochemistry	927:940	histochemistry	927:940	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	89	theme	stems	976:980	arg1	micromorphology					946:960	micromorphology	946:960	micromorphology	946:960	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	6	90	theme	oxygenated	1431:1440	arg1	%					1468:1468	14.31%-19.57%	1456:1468	14.31%-19.57%	1456:1468	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	90	theme	oxygenated	1431:1440	arg1	monoterpenes					1442:1453	oxygenated monoterpenes	1431:1453	oxygenated monoterpenes (14.31%-19.57%)	1431:1469	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	3	91	theme	gas	877:879	arg1	GC-MS					915:919	GC-MS	915:919	GC-MS	915:919	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	3	91	theme	gas	877:879	arg1	spectrometry					901:912	gas chromatography-mass spectrometry	877:912	gas chromatography-mass spectrometry (GC-MS)	877:920	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	4	92	theme	field	1032:1036	arg1	microscopy					1065:1074	field emission scanning electron microscopy	1032:1074	field emission scanning electron microscopy	1032:1074	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	4	92	theme	field	1032:1036	arg1	reagents					1012:1019	standard reagents	1003:1019	standard reagents	1003:1019	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	1	93	theme	monoterpenes	308:319	arg1	predominance					292:303	a predominance	290:303	a predominance of monoterpenes and sesquiterpenes	290:338	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	2	94	theme	objective	448:456	arg1	AIM					422:424	AIM	422:424	AIM OF STUDY This study's objective	422:456	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	2	95	from	effects	618:624	arg1	mice					629:632	mice	629:632	mice	629:632	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	6	96	from	monoterpenes	1368:1379	arg1	yield					1310:1314	its yield	1306:1314	its yield (0.40%-0.86%)	1306:1328	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	96	from	monoterpenes	1368:1379	arg1	composition					1343:1353	chemical composition	1334:1353	chemical composition	1334:1353	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	96	from	monoterpenes	1368:1379	arg1	%					1327:1327	0.40%-0.86%	1317:1327	0.40%-0.86%	1317:1327	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	2	97	theme	STUDY	429:433	arg1	objective					448:456	STUDY This study's objective	429:456	STUDY This study's objective	429:456	AIM OF STUDY This study's objective was to investigate the chemical composition of the essential oil of S. terebinthifolia (EOST) collected in six states of Brazil, evaluate its anti-inflammatory effects in mice, and analyze the histochemistry and micromorphology of leaves and stems.
36206869	6	98	theme	31.43	1415:1419	arg1	monoterpenes					1368:1379	hydrocarbon monoterpenes	1356:1379	hydrocarbon monoterpenes (28.76%-47.73%)	1356:1395	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	98	theme	31.43	1415:1419	arg1	%					1427:1427	31.43%-41.76%	1415:1427	31.43%-41.76%	1415:1427	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	5	99	theme	paw	1224:1226	arg1	methodology					1234:1244	the carrageenan-induced paw edema methodology	1200:1244	the carrageenan-induced paw edema methodology	1200:1244	The EOST were evaluated for anti-inflammatory activity and hyperalgesia using the carrageenan-induced paw edema methodology.
36206869	4	100	theme	X-ray	1102:1106	arg1	spectroscopy					1108:1119	energy-dispersive X-ray spectroscopy	1084:1119	energy-dispersive X-ray spectroscopy	1084:1119	The histochemistry and micromorphology of leaves and stems were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
36206869	1	101	dep	rich	250:253	arg1	used					357:360	used	357:360	being widely used in traditional medicine for the treatment of inflammations	344:419	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	1	102	theme	essential	258:266	arg1	oil					268:270	essential oil	258:270	essential oil	258:270	ETHNOPHARMACOLOGICAL RELEVANCE Schinus terebinthifolia Raddi (Anacardiaceae) is rich in essential oil, distinguished by a predominance of monoterpenes and sesquiterpenes, it being widely used in traditional medicine for the treatment of inflammations.
36206869	9	103	theme	anti-hyperalgesic	1859:1875	arg1	effects					1877:1883	antiedematogenic and anti-hyperalgesic effects	1838:1883	antiedematogenic and anti-hyperalgesic effects	1838:1883	In the in vivo testing, all EOST samples exerted antiedematogenic and anti-hyperalgesic effects, when tested in a carrageenan-induced paw inflammation (mechanical and thermal hyperalgesia) model with oral doses of 30 mg/kg.
36206869	3	104	theme	MATERIALS	707:715	arg1	parts					736:740	MATERIALS AND METHODS Aerial parts	707:740	MATERIALS AND METHODS Aerial parts of S. terebinthifolia	707:762	MATERIALS AND METHODS Aerial parts of S. terebinthifolia were collected in six states of Brazil, and the essential oil was extracted by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS).
36206869	6	105	theme	%	1461:1461	arg1	%					1468:1468	14.31%-19.57%	1456:1468	14.31%-19.57%	1456:1468	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36206869	6	105	theme	%	1461:1461	arg1	monoterpenes					1442:1453	oxygenated monoterpenes	1431:1453	oxygenated monoterpenes (14.31%-19.57%)	1431:1469	RESULTS The EOST showed variation across the six states in its yield (0.40%-0.86%) and chemical composition: hydrocarbon monoterpenes (28.76%-47.73%), sesquiterpenes, (31.43%-41.76%), oxygenated monoterpenes (14.31%-19.57%), and oxygenated sesquiterpenes (4.87%-14.38%).
36652798	0	0	theme	chitosan	72:79	arg1	film					96:99	chitosan food packaging film	72:99	chitosan food packaging film for fresh apple slice preservation	72:134	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	1	1	theme	carica	293:298	arg1	AgNPs					331:335	AgNPs	331:335	AgNPs	331:335	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	1	1	theme	carica	293:298	arg1	nanoparticles					316:328	Ficus carica mediated silver nanoparticles	287:328	Ficus carica mediated silver nanoparticles (AgNPs)	287:336	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	5	2	theme	Cht	806:808	arg1	film					810:813	Cht film	806:813	Cht film	806:813	The tensile strength of Cht film increased from 16 MPa to ∼24 MPa in Cht/KC/AgNPs film.
36652798	2	3	theme	Cht/KC/AgNPs	434:445	arg1	film					447:450	the Cht/KC/AgNPs film	430:450	the Cht/KC/AgNPs film	430:450	A comparison of the physico-chemical and functional characteristics of the Cht/KC/AgNPs film was performed with those of Cht, Cht/KC, and Cht/AgNPs.
36652798	1	4	theme	mediated	300:307	arg1	AgNPs					331:335	AgNPs	331:335	AgNPs	331:335	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	1	4	theme	mediated	300:307	arg1	nanoparticles					316:328	Ficus carica mediated silver nanoparticles	287:328	Ficus carica mediated silver nanoparticles (AgNPs)	287:336	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	9	5	theme	antibacterial	1332:1344	arg1	properties					1377:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	9	5	theme	antibacterial	1332:1344	arg1	barrier					1309:1315	good barrier	1304:1315	good barrier	1304:1315	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	2	6	theme	film	447:450	arg1	characteristics					411:425	the physico-chemical and functional characteristics	375:425	the physico-chemical and functional characteristics of the Cht/KC/AgNPs film	375:450	A comparison of the physico-chemical and functional characteristics of the Cht/KC/AgNPs film was performed with those of Cht, Cht/KC, and Cht/AgNPs.
36652798	5	7	theme	film	810:813	arg1	strength					794:801	The tensile strength	782:801	The tensile strength of Cht film	782:813	The tensile strength of Cht film increased from 16 MPa to ∼24 MPa in Cht/KC/AgNPs film.
36652798	6	8	theme	AgNPs	900:904	arg1	introduction					874:885	The introduction	870:885	The introduction of KC and/or AgNPs	870:904	The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film.
36652798	0	9	theme	packaging	86:94	arg1	film					96:99	chitosan food packaging film	72:99	chitosan food packaging film for fresh apple slice preservation	72:134	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	1	10	theme	silver	309:314	arg1	AgNPs					331:335	AgNPs	331:335	AgNPs	331:335	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	1	10	theme	silver	309:314	arg1	nanoparticles					316:328	Ficus carica mediated silver nanoparticles	287:328	Ficus carica mediated silver nanoparticles (AgNPs)	287:336	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	11	11	theme	slices	1769:1774	arg1	index					1699:1703	browning index	1690:1703	browning index	1690:1703	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	11	11	theme	slices	1769:1774	arg1	compound					1721:1728	total phenolic compound	1706:1728	total phenolic compound	1706:1728	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	11	11	theme	slices	1769:1774	arg1	loss					1684:1687	moisture loss	1675:1687	moisture loss	1675:1687	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	11	11	theme	slices	1769:1774	arg1	activity					1747:1754	antioxidant activity	1735:1754	antioxidant activity	1735:1754	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	12	12	theme	food	1916:1919	arg1	packaging					1921:1929	food packaging	1916:1929	food packaging	1916:1929	Moreover, the Cht/KC/AgNPs film exhibited a migration of silver meeting the standards set by EFSA and ECHA, which makes this film safe for food packaging.
36652798	9	13	theme	good	1304:1307	arg1	antioxidants					1318:1329	antioxidants	1318:1329	antioxidants	1318:1329	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	9	13	theme	good	1304:1307	arg1	properties					1377:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	9	13	theme	good	1304:1307	arg1	barrier					1309:1315	good barrier	1304:1315	good barrier	1304:1315	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	0	14	theme	food	81:84	arg1	film					96:99	chitosan food packaging film	72:99	chitosan food packaging film for fresh apple slice preservation	72:134	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	11	15	dep	loss	1684:1687	arg1	the					1671:1673	the	1671:1673	the	1671:1673	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	11	16	theme	total	1706:1710	arg1	compound					1721:1728	total phenolic compound	1706:1728	total phenolic compound	1706:1728	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	9	17	theme	synergistic	1402:1412	arg1	effect					1414:1419	the synergistic effect	1398:1419	the synergistic effect between KC and AgNPs	1398:1440	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	6	18	theme	KC	890:891	arg1	introduction					874:885	The introduction	870:885	The introduction of KC and/or AgNPs	870:904	The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film.
36652798	11	19	theme	phenolic	1712:1719	arg1	compound					1721:1728	total phenolic compound	1706:1728	total phenolic compound	1706:1728	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	7	20	theme	light	992:996	arg1	transmittance					998:1010	The UV light transmittance	985:1010	The UV light transmittance	985:1010	The UV light transmittance decreased by 50 % for Cht film when incorporated by KC and AgNPs.
36652798	3	21	contain	containing	567:576	arg2	particles					581:589	KC particles	578:589	KC particles	578:589	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	3	21	contain	containing	567:576	arg1	films					561:565	the composite films	547:565	the composite films containing KC particles because of their large diameter (50-120 μm)	547:633	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	6	22	theme	Cht	975:977	arg1	film					979:982	the Cht film	971:982	the Cht film	971:982	The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film.
36652798	3	23	dep	showed	521:526	arg1	compared					635:642	compared	635:642	showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm)	521:662	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	2	24	theme	functional	400:409	arg1	characteristics					411:425	the physico-chemical and functional characteristics	375:425	the physico-chemical and functional characteristics of the Cht/KC/AgNPs film	375:450	A comparison of the physico-chemical and functional characteristics of the Cht/KC/AgNPs film was performed with those of Cht, Cht/KC, and Cht/AgNPs.
36652798	9	25	attach	presented	1294:1302	arg1	particular					1260:1269	particular	1260:1269	particular	1260:1269	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	9	25	attach	presented	1294:1302	arg2	film					1289:1292	the Cht/KC/AgNPs film	1272:1292	the Cht/KC/AgNPs film	1272:1292	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	6	26	theme	light	932:936	arg1	capacity					959:966	the light and moisture barrier capacity	928:966	the light and moisture barrier capacity of the Cht film	928:982	The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film.
36652798	11	27	theme	promising	1639:1647	arg1	performance					1649:1659	promising performance	1639:1659	promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices	1639:1774	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	6	28	theme	film	979:982	arg1	capacity					959:966	the light and moisture barrier capacity	928:966	the light and moisture barrier capacity of the Cht film	928:982	The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film.
36652798	8	29	from	superior	1194:1201	arg1	biodegradability					1206:1221	biodegradability	1206:1221	biodegradability	1206:1221	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	8	29	from	superior	1194:1201	arg1	vapor					1233:1237	water vapor	1227:1237	water vapor	1227:1237	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	3	30	theme	SEM	508:510	arg1	analysis					512:519	SEM analysis	508:519	SEM analysis	508:519	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	1	31	theme	novel	153:157	arg1	film					220:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	2	32	theme	physico-chemical	379:394	arg1	characteristics					411:425	the physico-chemical and functional characteristics	375:425	the physico-chemical and functional characteristics of the Cht/KC/AgNPs film	375:450	A comparison of the physico-chemical and functional characteristics of the Cht/KC/AgNPs film was performed with those of Cht, Cht/KC, and Cht/AgNPs.
36652798	7	33	theme	UV	989:990	arg1	transmittance					998:1010	The UV light transmittance	985:1010	The UV light transmittance	985:1010	The UV light transmittance decreased by 50 % for Cht film when incorporated by KC and AgNPs.
36652798	8	34	theme	barrier	1239:1245	arg1	capacity					1247:1254	barrier capacity	1239:1254	barrier capacity	1239:1254	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	8	35	theme	antibacterial	1134:1146	arg1	terms					1112:1116	terms	1112:1116	terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity	1112:1254	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	1	36	theme	antioxidant	159:169	arg1	film					220:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	0	37	theme	clay	17:20	arg1	Effect					0:5	Effect	0:5	Effect of Kaolin clay and Ficus carica	0:37	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	1	38	theme	natural	258:264	arg1	KC					279:280	KC	279:280	KC	279:280	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	1	38	theme	natural	258:264	arg1	clay					273:276	natural kaolin clay	258:276	natural kaolin clay (KC)	258:281	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	0	39	theme	apple	111:115	arg1	preservation					123:134	fresh apple slice preservation	105:134	fresh apple slice preservation	105:134	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	4	40	theme	FTIR	669:672	arg1	analysis					674:681	The FTIR analysis	665:681	The FTIR analysis	665:681	The FTIR analysis suggested that the interactions between Cht and AgNPs were stronger than those between Cht and KC.
36652798	9	41	theme	mechanical	1347:1356	arg1	properties					1377:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	9	41	theme	mechanical	1347:1356	arg1	barrier					1309:1315	good barrier	1304:1315	good barrier	1304:1315	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	1	42	theme	kaolin	266:271	arg1	KC					279:280	KC	279:280	KC	279:280	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	1	42	theme	kaolin	266:271	arg1	clay					273:276	natural kaolin clay	258:276	natural kaolin clay (KC)	258:281	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	0	43	theme	fresh	105:109	arg1	preservation					123:134	fresh apple slice preservation	105:134	fresh apple slice preservation	105:134	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	1	44	theme	antibacterial	172:184	arg1	film					220:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	8	45	theme	mechanical	1153:1162	arg1	terms					1112:1116	terms	1112:1116	terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity	1112:1254	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	3	46	theme	rough	530:534	arg1	surface					536:542	a rough surface	528:542	a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm)	528:633	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	3	47	from	surface	536:542	arg1	films					561:565	the composite films	547:565	the composite films containing KC particles because of their large diameter (50-120 μm)	547:633	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	6	48	theme	barrier	951:957	arg1	capacity					959:966	the light and moisture barrier capacity	928:966	the light and moisture barrier capacity of the Cht film	928:982	The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film.
36652798	11	49	theme	apple	1763:1767	arg1	slices					1769:1774	the apple slices	1759:1774	the apple slices	1759:1774	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	11	50	theme	browning	1690:1697	arg1	index					1699:1703	browning index	1690:1703	browning index	1690:1703	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	0	51	theme	slice	117:121	arg1	preservation					123:134	fresh apple slice preservation	105:134	fresh apple slice preservation	105:134	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	11	52	theme	good	1597:1600	arg1	results					1602:1608	good results	1597:1608	good results	1597:1608	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	6	53	theme	moisture	942:949	arg1	capacity					959:966	the light and moisture barrier capacity	928:966	the light and moisture barrier capacity of the Cht film	928:982	The introduction of KC and/or AgNPs considerably improved the light and moisture barrier capacity of the Cht film.
36652798	2	54	theme	characteristics	411:425	arg1	comparison					361:370	A comparison	359:370	A comparison of the physico-chemical and functional characteristics of the Cht/KC/AgNPs film	359:450	A comparison of the physico-chemical and functional characteristics of the Cht/KC/AgNPs film was performed with those of Cht, Cht/KC, and Cht/AgNPs.
36652798	10	55	theme	packaging	1451:1459	arg1	properties					1461:1470	the packaging properties	1447:1470	the packaging properties	1447:1470	For the packaging properties, all the films were tested for their ability to keep the freshness of apple slices as wrapping material.
36652798	3	56	theme	KC	578:579	arg1	particles					581:589	KC particles	578:589	KC particles	578:589	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	8	57	theme	antioxidant	1121:1131	arg1	terms					1112:1116	terms	1112:1116	terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity	1112:1254	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	9	58	theme	biodegradable	1363:1375	arg1	properties					1377:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	antibacterial, mechanical, and biodegradable properties	1332:1386	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	9	58	theme	biodegradable	1363:1375	arg1	barrier					1309:1315	good barrier	1304:1315	good barrier	1304:1315	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36652798	0	59	theme	carica	32:37	arg1	Effect					0:5	Effect	0:5	Effect of Kaolin clay and Ficus carica	0:37	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	3	60	theme	large	608:612	arg1	50-120 μm					624:632	50-120 μm	624:632	50-120 μm	624:632	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	3	60	theme	large	608:612	arg1	diameter					614:621	their large diameter	602:621	their large diameter (50-120 μm)	602:633	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	12	61	theme	Cht/KC/AgNPs	1791:1802	arg1	film					1804:1807	the Cht/KC/AgNPs film	1787:1807	the Cht/KC/AgNPs film	1787:1807	Moreover, the Cht/KC/AgNPs film exhibited a migration of silver meeting the standards set by EFSA and ECHA, which makes this film safe for food packaging.
36652798	8	62	from	vapor	1233:1237	arg1	superior					1194:1201	superior	1194:1201	superior	1194:1201	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	7	63	theme	Cht	1034:1036	arg1	film					1038:1041	Cht film	1034:1041	Cht film	1034:1041	The UV light transmittance decreased by 50 % for Cht film when incorporated by KC and AgNPs.
36652798	3	64	theme	composite	551:559	arg1	films					561:565	the composite films	547:565	the composite films containing KC particles because of their large diameter (50-120 μm)	547:633	SEM analysis showed a rough surface in the composite films containing KC particles because of their large diameter (50-120 μm) compared to AgNPs (20-80 nm).
36652798	5	65	theme	tensile	786:792	arg1	strength					794:801	The tensile strength	782:801	The tensile strength of Cht film	782:813	The tensile strength of Cht film increased from 16 MPa to ∼24 MPa in Cht/KC/AgNPs film.
36652798	1	66	theme	biodegradable	191:203	arg1	film					220:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	8	67	theme	water	1227:1231	arg1	vapor					1233:1237	water vapor	1227:1237	water vapor	1227:1237	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	11	68	theme	moisture	1675:1682	arg1	loss					1684:1687	moisture loss	1675:1687	moisture loss	1675:1687	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	10	69	theme	slices	1548:1553	arg1	freshness					1529:1537	the freshness	1525:1537	the freshness of apple slices	1525:1553	For the packaging properties, all the films were tested for their ability to keep the freshness of apple slices as wrapping material.
36652798	1	70	theme	food	205:208	arg1	film					220:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	8	71	dep	biodegradability	1206:1221	arg1	capacity					1247:1254	barrier capacity	1239:1254	barrier capacity	1239:1254	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	10	72	theme	apple	1542:1546	arg1	slices					1548:1553	apple slices	1542:1553	apple slices	1542:1553	For the packaging properties, all the films were tested for their ability to keep the freshness of apple slices as wrapping material.
36652798	11	73	theme	antioxidant	1735:1745	arg1	activity					1747:1754	antioxidant activity	1735:1754	antioxidant activity	1735:1754	The films exhibited good results, and the Cht/KC/AgNPs showed promising performance regarding the moisture loss, browning index, total phenolic compound, and antioxidant activity of the apple slices.
36652798	8	74	from	biodegradability	1206:1221	arg1	superior					1194:1201	superior	1194:1201	superior	1194:1201	Moreover, Cht/AgNPs was better in terms of antioxidant, antibacterial, and mechanical compared to Cht/KC, which was superior in biodegradability and water vapor barrier capacity.
36652798	1	75	theme	packaging	210:218	arg1	film					220:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	a novel antioxidant, antibacterial, and biodegradable food packaging film	151:223	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	0	76	theme	silver	48:53	arg1	nanoparticles					55:67	silver nanoparticles	48:67	silver nanoparticles	48:67	Effect of Kaolin clay and Ficus carica mediated silver nanoparticles on chitosan food packaging film for fresh apple slice preservation.
36652798	1	77	theme	Ficus	287:291	arg1	AgNPs					331:335	AgNPs	331:335	AgNPs	331:335	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	1	77	theme	Ficus	287:291	arg1	nanoparticles					316:328	Ficus carica mediated silver nanoparticles	287:328	Ficus carica mediated silver nanoparticles (AgNPs)	287:336	In this work, a novel antioxidant, antibacterial, and biodegradable food packaging film was elaborated, by incorporating natural kaolin clay (KC) and Ficus carica mediated silver nanoparticles (AgNPs) into Chitosan (Cht).
36652798	9	78	theme	Cht/KC/AgNPs	1276:1287	arg1	film					1289:1292	the Cht/KC/AgNPs film	1272:1292	the Cht/KC/AgNPs film	1272:1292	In particular, the Cht/KC/AgNPs film presented good barrier, antioxidants, antibacterial, mechanical, and biodegradable properties, owing to the synergistic effect between KC and AgNPs.
36709819	0	0	theme	food	109:112	arg1	packaging					128:136	food antibacterial packaging	109:136	food antibacterial packaging	109:136	Insights into electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers loaded with nanogels for food antibacterial packaging.
36709819	8	1	theme	antimicrobial	1477:1489	arg1	stability					1491:1499	antimicrobial stability	1477:1499	antimicrobial stability	1477:1499	Taken together, the CNNGs can be well stabilized with the core-shell nanofibers, thus exerting significantly improved antimicrobial stability and bioactivity.
36709819	9	2	theme	special	1523:1529	arg1	structure					1531:1539	This special structure	1518:1539	This special structure	1518:1539	This special structure exerts a great potential for application as food packaging materials to preserve aquatic products.
36709819	4	3	theme	core	833:836	arg1	layer					838:842	the core layer	829:842	the core layer of the nanofibers as antibacterial agents	829:884	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	1	4	from	lactis	183:188	arg1	substance					156:164	a natural substance	146:164	a natural substance from Lactococcus lactis	146:188	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	1	4	from	lactis	183:188	arg1	Nisin					139:143	Nisin	139:143	Nisin	139:143	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	9	5	theme	great	1550:1554	arg1	potential					1556:1564	a great potential	1548:1564	a great potential for application as food packaging materials	1548:1608	This special structure exerts a great potential for application as food packaging materials to preserve aquatic products.
36709819	9	6	theme	food	1585:1588	arg1	materials					1600:1608	food packaging materials	1585:1608	food packaging materials	1585:1608	This special structure exerts a great potential for application as food packaging materials to preserve aquatic products.
36709819	0	7	theme	antibacterial	114:126	arg1	packaging					128:136	food antibacterial packaging	109:136	food antibacterial packaging	109:136	Insights into electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers loaded with nanogels for food antibacterial packaging.
36709819	4	8	theme	shell	706:710	arg1	layer					712:716	shell layer	706:716	shell layer	706:716	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	9	theme	core	633:636	arg1	layer					638:642	a core layer	631:642	a core layer	631:642	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	2	10	theme	external	304:311	arg1	environment					313:323	the external environment	300:323	the external environment	300:323	However, it is susceptible to the external environment, i.e. temperature, pH, and food composition.
36709819	6	11	theme	enhanced	1039:1046	arg1	stability					1056:1064	thermal stability	1048:1064	thermal stability	1048:1064	The CNNGs-loaded nanofibers exhibited enhanced thermal stability and mechanical strength, as well as excellent antibacterial activity.
36709819	4	12	theme	core-shell	592:601	arg1	nanofibers					603:612	The core-shell nanofibers	588:612	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer	588:716	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	3	13	theme	coaxial	414:420	arg1	method					406:411	a dual stabilization method	385:411	a dual stabilization method	385:411	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	3	13	theme	coaxial	414:420	arg1	electrospinning					422:436	coaxial electrospinning	414:436	coaxial electrospinning	414:436	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	4	14	theme	prepared	746:753	arg1	CNNGs					776:780	CNNGs	776:780	CNNGs	776:780	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	14	theme	prepared	746:753	arg1	nanogels					766:773	the prepared CMCS-nisin nanogels	742:773	the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method	742:810	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	6	15	theme	mechanical	1070:1079	arg1	strength					1081:1088	mechanical strength	1070:1088	mechanical strength	1070:1088	The CNNGs-loaded nanofibers exhibited enhanced thermal stability and mechanical strength, as well as excellent antibacterial activity.
36709819	3	16	theme	dual	387:390	arg1	method					406:411	a dual stabilization method	385:411	a dual stabilization method	385:411	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	3	16	theme	dual	387:390	arg1	electrospinning					422:436	coaxial electrospinning	414:436	coaxial electrospinning	414:436	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	7	17	with	CNNGSs	1262:1267	arg1	nisin					1274:1278	nisin	1274:1278	nisin	1274:1278	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	1	18	theme	promising	202:210	arg1	ability					226:232	a promising antibacterial ability	200:232	a promising antibacterial ability against the gram-positive bacteria	200:267	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	3	19	theme	stabilization	392:404	arg1	method					406:411	a dual stabilization method	385:411	a dual stabilization method	385:411	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	3	19	theme	stabilization	392:404	arg1	electrospinning					422:436	coaxial electrospinning	414:436	coaxial electrospinning	414:436	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	1	20	theme	antibacterial	212:224	arg1	ability					226:232	a promising antibacterial ability	200:232	a promising antibacterial ability against the gram-positive bacteria	200:267	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	0	21	theme	pullulan-carboxymethyl	26:47	arg1	nanofibers					73:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	Insights into electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers loaded with nanogels for food antibacterial packaging.
36709819	5	22	theme	smooth	916:921	arg1	surface					923:929	the smooth surface	912:929	the smooth surface	912:929	The result revealed that the smooth surface can be observed on the nanofibers by microstructure characterization.
36709819	3	23	from	effect	504:509	arg1	properties					541:550	the properties	537:550	the properties of the nanofibers	537:568	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	6	24	theme	thermal	1048:1054	arg1	stability					1056:1064	thermal stability	1048:1064	thermal stability	1048:1064	The CNNGs-loaded nanofibers exhibited enhanced thermal stability and mechanical strength, as well as excellent antibacterial activity.
36709819	0	25	theme	electrospun	14:24	arg1	nanofibers					73:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	Insights into electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers loaded with nanogels for food antibacterial packaging.
36709819	4	26	theme	CMCS-nisin	755:764	arg1	CNNGs					776:780	CNNGs	776:780	CNNGs	776:780	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	26	theme	CMCS-nisin	755:764	arg1	nanogels					766:773	the prepared CMCS-nisin nanogels	742:773	the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method	742:810	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	8	27	theme	core-shell	1417:1426	arg1	nanofibers					1428:1437	the core-shell nanofibers	1413:1437	the core-shell nanofibers	1413:1437	Taken together, the CNNGs can be well stabilized with the core-shell nanofibers, thus exerting significantly improved antimicrobial stability and bioactivity.
36709819	3	28	theme	nanofibers	559:568	arg1	properties					541:550	the properties	537:550	the properties of the nanofibers	537:568	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	3	29	theme	food	471:474	arg1	materials					486:494	food packaging materials	471:494	food packaging materials	471:494	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	0	30	theme	core-shell	62:71	arg1	nanofibers					73:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	Insights into electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers loaded with nanogels for food antibacterial packaging.
36709819	5	31	located	observed	938:945	arg2	surface					923:929	the smooth surface	912:929	the smooth surface	912:929	The result revealed that the smooth surface can be observed on the nanofibers by microstructure characterization.
36709819	5	31	located	observed	938:945	arg1	nanofibers					954:963	the nanofibers	950:963	the nanofibers by microstructure characterization	950:998	The result revealed that the smooth surface can be observed on the nanofibers by microstructure characterization.
36709819	7	32	theme	bass	1242:1245	arg1	fish					1247:1250	bass fish	1242:1250	bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL	1242:1308	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	9	33	theme	packaging	1590:1598	arg1	materials					1600:1608	food packaging materials	1585:1608	food packaging materials	1585:1608	This special structure exerts a great potential for application as food packaging materials to preserve aquatic products.
36709819	7	34	theme	8 mg/mL	1302:1308	arg1	concentration					1285:1297	a concentration	1283:1297	a concentration of 8 mg/mL	1283:1308	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	4	35	theme	carboxymethyl	648:660	arg1	CMCS					672:675	CMCS	672:675	CMCS	672:675	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	35	theme	carboxymethyl	648:660	arg1	chitosan					662:669	carboxymethyl chitosan	648:669	carboxymethyl chitosan (CMCS)	648:676	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	0	36	theme	chitosan/PEO	49:60	arg1	nanofibers					73:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers	14:82	Insights into electrospun pullulan-carboxymethyl chitosan/PEO core-shell nanofibers loaded with nanogels for food antibacterial packaging.
36709819	7	37	theme	as-formed	1153:1161	arg1	nanofibers					1163:1172	the as-formed nanofibers	1149:1172	the as-formed nanofibers	1149:1172	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	7	38	theme	bass	1199:1202	arg1	fish					1204:1207	bass fish	1199:1207	bass fish	1199:1207	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	1	39	theme	gram-positive	246:258	arg1	bacteria					260:267	the gram-positive bacteria	242:267	the gram-positive bacteria	242:267	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	4	40	dep	layer	638:642	arg1	PEO					698:700	PEO	698:700	PEO	698:700	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	40	dep	layer	638:642	arg1	oxide					691:695	/polyethylene oxide	677:695	/polyethylene oxide (PEO)	677:701	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	6	41	theme	antibacterial	1112:1124	arg1	activity					1126:1133	excellent antibacterial activity	1102:1133	excellent antibacterial activity	1102:1133	The CNNGs-loaded nanofibers exhibited enhanced thermal stability and mechanical strength, as well as excellent antibacterial activity.
36709819	2	42	dep	temperature	331:341	arg1	i.e.					326:329	i.e.	326:329	i.e.	326:329	However, it is susceptible to the external environment, i.e. temperature, pH, and food composition.
36709819	4	43	theme	nanofibers	851:860	arg1	layer					838:842	the core layer	829:842	the core layer of the nanofibers as antibacterial agents	829:884	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	44	theme	self-assembly	791:803	arg1	method					805:810	a self-assembly method	789:810	a self-assembly method	789:810	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	2	45	theme	food	352:355	arg1	composition					357:367	food composition	352:367	food composition	352:367	However, it is susceptible to the external environment, i.e. temperature, pH, and food composition.
36709819	7	46	theme	fish	1247:1250	arg1	life					1234:1237	the shelf life	1224:1237	the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL	1224:1308	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	9	47	theme	aquatic	1622:1628	arg1	products					1630:1637	aquatic products	1622:1637	aquatic products	1622:1637	This special structure exerts a great potential for application as food packaging materials to preserve aquatic products.
36709819	4	48	with	nanofibers	603:612	arg1	pullulan					619:626	pullulan	619:626	pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO)	619:701	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	49	theme	/polyethylene	677:689	arg1	PEO					698:700	PEO	698:700	PEO	698:700	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	4	49	theme	/polyethylene	677:689	arg1	oxide					691:695	/polyethylene oxide	677:695	/polyethylene oxide (PEO)	677:701	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	1	50	theme	natural	148:154	arg1	substance					156:164	a natural substance	146:164	a natural substance from Lactococcus lactis	146:188	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	1	50	theme	natural	148:154	arg1	Nisin					139:143	Nisin	139:143	Nisin	139:143	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	6	51	theme	excellent	1102:1110	arg1	activity					1126:1133	excellent antibacterial activity	1102:1133	excellent antibacterial activity	1102:1133	The CNNGs-loaded nanofibers exhibited enhanced thermal stability and mechanical strength, as well as excellent antibacterial activity.
36709819	6	52	theme	CNNGs-loaded	1005:1016	arg1	nanofibers					1018:1027	The CNNGs-loaded nanofibers	1001:1027	The CNNGs-loaded nanofibers	1001:1027	The CNNGs-loaded nanofibers exhibited enhanced thermal stability and mechanical strength, as well as excellent antibacterial activity.
36709819	4	53	theme	antibacterial	865:877	arg1	agents					879:884	antibacterial agents	865:884	antibacterial agents	865:884	The core-shell nanofibers with pullulan as a core layer and carboxymethyl chitosan (CMCS)/polyethylene oxide (PEO) as shell layer were prepared, and then the prepared CMCS-nisin nanogels (CNNGs) using a self-assembly method were loaded into the core layer of the nanofibers as antibacterial agents.
36709819	8	54	theme	improved	1468:1475	arg1	stability					1491:1499	antimicrobial stability	1477:1499	antimicrobial stability	1477:1499	Taken together, the CNNGs can be well stabilized with the core-shell nanofibers, thus exerting significantly improved antimicrobial stability and bioactivity.
36709819	1	55	theme	Lactococcus	171:181	arg1	lactis					183:188	Lactococcus lactis	171:188	Lactococcus lactis	171:188	Nisin, a natural substance from Lactococcus lactis, displays a promising antibacterial ability against the gram-positive bacteria.
36709819	3	56	theme	packaging	476:484	arg1	materials					486:494	food packaging materials	471:494	food packaging materials	471:494	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	7	57	from	concentration	1285:1297	arg1	CNNGSs					1262:1267	CNNGSs	1262:1267	CNNGSs with nisin at a concentration of 8 mg/mL	1262:1308	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	3	58	theme	nisin	514:518	arg1	concentration					520:532	nisin concentration	514:532	nisin concentration	514:532	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
36709819	7	59	theme	shelf	1228:1232	arg1	life					1234:1237	the shelf life	1224:1237	the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL	1224:1308	Importantly, the as-formed nanofibers were applied to preserve bass fish and found that the shelf life of bass fish packed by CNNGSs with nisin at a concentration of 8 mg/mL was effectively extended from 9 days to 15 days.
36709819	5	60	theme	microstructure	968:981	arg1	characterization					983:998	microstructure characterization	968:998	microstructure characterization	968:998	The result revealed that the smooth surface can be observed on the nanofibers by microstructure characterization.
36709819	3	61	theme	concentration	520:532	arg1	effect					504:509	the effect	500:509	the effect of nisin concentration on the properties of the nanofibers	500:568	In this study, a dual stabilization method, coaxial electrospinning, was applied to protect nisin in food packaging materials and the effect of nisin concentration on the properties of the nanofibers was investigated.
35969991	2	0	theme	in	524:525	arg1	fermentation					543:554	in vitro pig fecal fermentation	524:554	in vitro pig fecal fermentation	524:554	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	2	1	attach	derived	375:381	arg1	galacto-oligosaccharides					438:461	galacto-oligosaccharides	438:461	galacto-oligosaccharides	438:461	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	2	1	attach	derived	375:381	arg2	MRPs					370:373	MRPs	370:373	MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides	370:461	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	2	1	attach	derived	375:381	arg1	hydrolysates					406:417	bighead carp meat hydrolysates	388:417	bighead carp meat hydrolysates with galactose	388:432	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	0	2	theme	fecal	187:191	arg1	fermentation					193:204	in vitro pig fecal fermentation	174:204	in vitro pig fecal fermentation	174:204	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	3	3	from	dominant	823:830	arg1	treatment					842:850	the GM treatment	835:850	the GM treatment	835:850	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	4	with	BCH	913:915	arg1	galactose					922:930	galactose	922:930	galactose	922:930	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	0	5	theme	pig	183:185	arg1	fermentation					193:204	in vitro pig fecal fermentation	174:204	in vitro pig fecal fermentation	174:204	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	0	6	theme	bighead	92:98	arg1	hydrolysates					110:121	bighead carp meat hydrolysates	92:121	bighead carp meat hydrolysates with galactose and galacto-oligosaccharides	92:165	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	2	7	from	effects	359:365	arg1	composition					487:497	intestinal microbial composition	466:497	intestinal microbial composition	466:497	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	2	7	from	effects	359:365	arg1	profile					513:519	metabolic profile	503:519	metabolic profile	503:519	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	3	8	theme	glycated	904:911	arg1	LgM					899:901	LgM	899:901	LgM (glycated BCH with galactose) treatment	899:941	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	8	theme	glycated	904:911	arg1	BCH					913:915	glycated BCH	904:915	glycated BCH with galactose	904:930	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	1	9	theme	gut	263:265	arg1	health					267:272	gut health	263:272	gut health	263:272	Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions.
35969991	3	10	with	BCH	672:674	arg1	galacto-oligosaccharide					681:703	galacto-oligosaccharide	681:703	galacto-oligosaccharide	681:703	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	0	11	theme	meat	105:108	arg1	hydrolysates					110:121	bighead carp meat hydrolysates	92:121	bighead carp meat hydrolysates with galactose and galacto-oligosaccharides	92:165	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	2	12	with	hydrolysates	406:417	arg1	galactose					424:432	galactose	424:432	galactose	424:432	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	3	13	from	treatment	933:941	arg1	predominant					884:894	predominant	884:894	predominant	884:894	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	2	14	theme	meat	401:404	arg1	hydrolysates					406:417	bighead carp meat hydrolysates	388:417	bighead carp meat hydrolysates with galactose	388:432	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	0	15	theme	carp	100:103	arg1	hydrolysates					110:121	bighead carp meat hydrolysates	92:121	bighead carp meat hydrolysates with galactose and galacto-oligosaccharides	92:165	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	2	16	theme	intestinal	466:475	arg1	composition					487:497	intestinal microbial composition	466:497	intestinal microbial composition	466:497	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	2	17	theme	carp	396:399	arg1	hydrolysates					406:417	bighead carp meat hydrolysates	388:417	bighead carp meat hydrolysates with galactose	388:432	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	2	18	theme	microbial	477:485	arg1	composition					487:497	intestinal microbial composition	466:497	intestinal microbial composition	466:497	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	5	19	theme	acids	1204:1208	arg1	production					1167:1176	the production	1163:1176	the production of butyric acid and fatty acids synthesis	1163:1218	Overall, GM will be more beneficial for gut health by promoting the production of butyric acid and fatty acids synthesis.
35969991	2	20	theme	pig	533:535	arg1	fermentation					543:554	in vitro pig fecal fermentation	524:554	in vitro pig fecal fermentation	524:554	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	2	21	theme	bighead	388:394	arg1	hydrolysates					406:417	bighead carp meat hydrolysates	388:417	bighead carp meat hydrolysates with galactose	388:432	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	0	22	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota	0:13	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	5	23	theme	acid	1189:1192	arg1	production					1167:1176	the production	1163:1176	the production of butyric acid and fatty acids synthesis	1163:1218	Overall, GM will be more beneficial for gut health by promoting the production of butyric acid and fatty acids synthesis.
35969991	3	24	from	predominant	884:894	arg1	treatment					933:941	LgM (glycated BCH with galactose) treatment	899:941	LgM (glycated BCH with galactose) treatment	899:941	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	0	25	theme	metabolic	19:27	arg1	profile					29:35	metabolic profile	19:35	metabolic profile	19:35	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	3	26	theme	butyric	630:636	arg1	acid					638:641	butyric acid	630:641	butyric acid	630:641	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	27	from	treatment	842:850	arg1	dominant					823:830	dominant	823:830	dominant	823:830	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	1	28	theme	Maillard	207:214	arg1	MRPs					235:238	MRPs	235:238	MRPs	235:238	Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions.
35969991	1	28	theme	Maillard	207:214	arg1	products					225:232	Maillard reaction products	207:232	Maillard reaction products (MRPs)	207:239	Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions.
35969991	5	29	theme	fatty	1198:1202	arg1	acids					1204:1208	fatty acids	1198:1208	fatty acids	1198:1208	Overall, GM will be more beneficial for gut health by promoting the production of butyric acid and fatty acids synthesis.
35969991	3	30	theme	first	589:593	arg1	12 h					595:598	the first 12 h	585:598	the first 12 h	585:598	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	1	31	theme	reaction	216:223	arg1	MRPs					235:238	MRPs	235:238	MRPs	235:238	Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions.
35969991	1	31	theme	reaction	216:223	arg1	products					225:232	Maillard reaction products	207:232	Maillard reaction products (MRPs)	207:239	Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions.
35969991	1	32	theme	microbiome-host	291:305	arg1	interactions					307:318	the microbiome-host interactions	287:318	the microbiome-host interactions	287:318	Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions.
35969991	4	33	theme	up-regulated	956:967	arg1	metabolites					969:979	The up-regulated metabolites	952:979	The up-regulated metabolites	952:979	The up-regulated metabolites indicated that GM and LgM might participate in the fatty acids synthesis and modulate lipid metabolism, respectively.
35969991	3	34	theme	glycated	663:670	arg1	GM					659:660	GM	659:660	GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05)	659:746	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	34	theme	glycated	663:670	arg1	BCH					672:674	glycated BCH	663:674	glycated BCH with galacto-oligosaccharide	663:703	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	35	from	12 h	946:949	arg1	predominant					884:894	predominant	884:894	predominant	884:894	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	2	36	theme	metabolic	503:511	arg1	profile					513:519	metabolic profile	503:519	metabolic profile	503:519	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	4	37	theme	lipid	1067:1071	arg1	metabolism					1073:1082	lipid metabolism	1067:1082	lipid metabolism	1067:1082	The up-regulated metabolites indicated that GM and LgM might participate in the fatty acids synthesis and modulate lipid metabolism, respectively.
35969991	2	38	theme	fecal	537:541	arg1	fermentation					543:554	in vitro pig fecal fermentation	524:554	in vitro pig fecal fermentation	524:554	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	4	39	theme	acids	1038:1042	arg1	synthesis					1044:1052	the fatty acids synthesis	1028:1052	the fatty acids synthesis	1028:1052	The up-regulated metabolites indicated that GM and LgM might participate in the fatty acids synthesis and modulate lipid metabolism, respectively.
35969991	2	40	dep	in	524:525	arg1	vitro					527:531	vitro	527:531	vitro	527:531	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	3	41	theme	GM	659:660	arg1	treatment					706:714	GM (glycated BCH with galacto-oligosaccharide) treatment	659:714	GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05)	659:746	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	2	42	with	galacto-oligosaccharides	438:461	arg1	galactose					424:432	galactose	424:432	galactose	424:432	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	5	43	dep	acid	1189:1192	arg1	synthesis					1210:1218	synthesis	1210:1218	synthesis	1210:1218	Overall, GM will be more beneficial for gut health by promoting the production of butyric acid and fatty acids synthesis.
35969991	4	44	theme	fatty	1032:1036	arg1	synthesis					1044:1052	the fatty acids synthesis	1028:1052	the fatty acids synthesis	1028:1052	The up-regulated metabolites indicated that GM and LgM might participate in the fatty acids synthesis and modulate lipid metabolism, respectively.
35969991	3	45	theme	highest	608:614	arg1	production					616:625	the highest production	604:625	the highest production of butyric acid	604:641	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	0	46	dep	in	174:175	arg1	vitro					177:181	vitro	177:181	vitro	177:181	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	3	47	located	observed	647:654	arg1	treatment					706:714	GM (glycated BCH with galacto-oligosaccharide) treatment	659:714	GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05)	659:746	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	47	located	observed	647:654	arg1	Streptococcus					786:798	Streptococcus	786:798	Streptococcus	786:798	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	47	located	observed	647:654	arg2	production					616:625	the highest production	604:625	the highest production of butyric acid	604:641	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	5	48	theme	butyric	1181:1187	arg1	acid					1189:1192	butyric acid	1181:1192	butyric acid	1181:1192	Overall, GM will be more beneficial for gut health by promoting the production of butyric acid and fatty acids synthesis.
35969991	0	49	attach	derived	79:85	arg1	hydrolysates					110:121	bighead carp meat hydrolysates	92:121	bighead carp meat hydrolysates with galactose and galacto-oligosaccharides	92:165	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	0	49	attach	derived	79:85	arg2	products					70:77	Maillard reaction products	52:77	Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation	52:204	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	3	50	theme	LgM	899:901	arg1	treatment					933:941	LgM (glycated BCH with galactose) treatment	899:941	LgM (glycated BCH with galactose) treatment	899:941	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	0	51	theme	in	174:175	arg1	fermentation					193:204	in vitro pig fecal fermentation	174:204	in vitro pig fecal fermentation	174:204	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	3	52	theme	GM	839:840	arg1	treatment					842:850	the GM treatment	835:850	the GM treatment	835:850	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	0	53	theme	reaction	61:68	arg1	products					70:77	Maillard reaction products	52:77	Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation	52:204	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	3	54	attach	predominant	884:894	arg2	Escherichia-Shigella					859:878	Escherichia-Shigella	859:878	Escherichia-Shigella	859:878	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	54	attach	predominant	884:894	arg1	treatment					933:941	LgM (glycated BCH with galactose) treatment	899:941	LgM (glycated BCH with galactose) treatment	899:941	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	54	attach	predominant	884:894	arg1	12 h					946:949	12 h	946:949	12 h	946:949	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	0	55	with	hydrolysates	110:121	arg1	galactose					128:136	galactose	128:136	galactose	128:136	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	0	55	with	hydrolysates	110:121	arg1	galacto-oligosaccharides					142:165	galacto-oligosaccharides	142:165	galacto-oligosaccharides	142:165	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	0	56	theme	Maillard	52:59	arg1	products					70:77	Maillard reaction products	52:77	Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation	52:204	Gut microbiota and metabolic profile as affected by Maillard reaction products derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides during in vitro pig fecal fermentation.
35969991	5	57	theme	gut	1139:1141	arg1	health					1143:1148	gut health	1139:1148	gut health	1139:1148	Overall, GM will be more beneficial for gut health by promoting the production of butyric acid and fatty acids synthesis.
35969991	3	58	theme	acid	638:641	arg1	production					616:625	the highest production	604:625	the highest production of butyric acid	604:641	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	2	59	theme	MRPs	370:373	arg1	effects					359:365	the effects	355:365	the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile	355:519	This study aimed at investigating the effects of MRPs derived from bighead carp meat hydrolysates with galactose and galacto-oligosaccharides on intestinal microbial composition and metabolic profile by in vitro pig fecal fermentation.
35969991	3	60	with	treatment	706:714	arg1	p < 0.05					738:745	p < 0.05	738:745	p < 0.05	738:745	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	3	60	with	treatment	706:714	arg1	μmoL/10 mL					726:735	64.7 μmoL/10 mL	721:735	64.7 μmoL/10 mL (p < 0.05)	721:746	The pH decreased sharply in the first 12 h and the highest production of butyric acid was observed in GM (glycated BCH with galacto-oligosaccharide) treatment with 64.7 μmoL/10 mL (p < 0.05) at 48 h. Clostridium_sensu_stricto_1, Streptococcus, and Enterococcus were dominant in the GM treatment, while Escherichia-Shigella was predominant in LgM (glycated BCH with galactose) treatment at 12 h.
35969991	1	61	theme	pivotal	246:252	arg1	roles					254:258	pivotal roles	246:258	pivotal roles	246:258	Maillard reaction products (MRPs) play pivotal roles in gut health by affecting the microbiome-host interactions.
36191051	5	0	theme	strains	263:269	arg1	Cells					248:252	Cells	248:252	Cells of these strains	248:269	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	7	1	theme	16S	857:859	arg1	sequence					871:878	16S rRNA gene sequence	857:878	16S rRNA gene sequence	857:878	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	7	2	theme	genomes	717:723	arg1	values					675:680	Similarity values	664:680	Similarity values	664:680	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	5	3	theme	Gram-stain-negative	276:294	arg1	structures					377:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures	276:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility	276:399	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	11	4	theme	Anaeromyxobacter	1491:1506	arg1	sp					1523:1524	Anaeromyxobacter diazotrophicus sp	1491:1524	Anaeromyxobacter diazotrophicus sp	1491:1524	nov., Anaeromyxobacter diazotrophicus sp.
36191051	11	4	theme	Anaeromyxobacter	1491:1506	arg1	nov.					1485:1488	nov.	1485:1488	nov.	1485:1488	nov., Anaeromyxobacter diazotrophicus sp.
36191051	7	5	theme	sequences	703:711	arg1	values					675:680	Similarity values	664:680	Similarity values	664:680	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	8	6	theme	novel	1137:1141	arg1	strains					1143:1149	the three novel strains	1127:1149	the three novel strains	1127:1149	Nitrogenase genes were observed in the genomes of the three novel strains, but not in A. dehalogenans 2CP-1T.
36191051	6	7	theme	single-copy	497:507	arg1	sequences					514:522	multiple single-copy gene sequences	488:522	multiple single-copy gene sequences	488:522	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	10	8	theme	novel	1387:1391	arg1	species					1405:1411	three novel independent species	1381:1411	three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp	1381:1482	Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp.
36191051	4	9	attach	isolated	204:211	arg2	Red630T					190:196	Red630T	190:196	Red630T	190:196	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	4	9	attach	isolated	204:211	arg1	soils					224:228	paddy soils	218:228	paddy soils sampled in Japan	218:245	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	4	9	attach	isolated	204:211	arg2	Red267T					178:184	Red267T	178:184	Red267T	178:184	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	4	9	attach	isolated	204:211	arg2	strains					160:166	Three bacterial strains	144:166	Three bacterial strains (Red232T, Red267T and Red630T)	144:197	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	7	10	theme	species	1045:1051	arg1	delineation					1053:1063	the species delineation	1041:1063	the species delineation thresholds	1041:1074	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	9	11	theme	genomic	1206:1212	arg1	features					1248:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	Moreover, multiple genomic, physiological and chemotaxonomic features supported the discrimination between these three strains.
36191051	7	12	theme	2CP-1T	829:834	arg1	strains					750:756	the three isolated strains	731:756	the three isolated strains	731:756	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	7	12	theme	2CP-1T	829:834	arg1	strain					771:776	the type strain	762:776	the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T	762:834	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	5	13	theme	rod-shaped	325:334	arg1	structures					377:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures	276:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility	276:399	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	7	14	theme	16S	689:691	arg1	sequences					703:711	the 16S rRNA gene sequences	685:711	the 16S rRNA gene sequences	685:711	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	8	15	theme	A.	1163:1164	arg1	2CP-1T					1179:1184	A. dehalogenans 2CP-1T	1163:1184	A. dehalogenans 2CP-1T	1163:1184	Nitrogenase genes were observed in the genomes of the three novel strains, but not in A. dehalogenans 2CP-1T.
36191051	5	16	theme	monotrichous	341:352	arg1	flagella					354:361	monotrichous flagella	341:361	monotrichous flagella	341:361	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	14	17	theme	114076T=MCCC	1691:1702	arg1	1K03957T					1704:1711	=NBRC 114076T=MCCC 1K03957T	1685:1711	=NBRC 114076T=MCCC 1K03957T	1685:1711	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	14	17	theme	114076T=MCCC	1691:1702	arg1	Red630T					1676:1682	Red630T	1676:1682	Red630T (=NBRC 114076T=MCCC 1K03957T)	1676:1712	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	7	18	theme	average	989:995	arg1	identity					1008:1015	average amino acid identity	989:1015	average amino acid identity	989:1015	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	6	19	theme	multiple	488:495	arg1	sequences					514:522	multiple single-copy gene sequences	488:522	multiple single-copy gene sequences	488:522	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	7	20	theme	Similarity	664:673	arg1	values					675:680	Similarity values	664:680	Similarity values	664:680	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	7	21	theme	nucleotide	904:913	arg1	identity					915:922	average nucleotide identity	896:922	average nucleotide identity	896:922	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	6	22	theme	other	643:647	arg1	genera					656:661	any other strain genera	639:661	any other strain genera	639:661	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	14	23	theme	=NBRC	1642:1646	arg1	1K04211T					1661:1668	=NBRC 114075T=MCCC 1K04211T	1642:1668	=NBRC 114075T=MCCC 1K04211T	1642:1668	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	14	23	theme	=NBRC	1642:1646	arg1	Red267T					1633:1639	Red267T	1633:1639	Red267T (=NBRC 114075T=MCCC 1K04211T)	1633:1669	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	12	24	theme	nov.	1527:1530	arg1	paludicola					1553:1562	nov. and Anaeromyxobacter paludicola	1527:1562	nov. and Anaeromyxobacter paludicola	1527:1562	nov. and Anaeromyxobacter paludicola sp.
36191051	7	25	theme	gene	698:701	arg1	sequences					703:711	the 16S rRNA gene sequences	685:711	the 16S rRNA gene sequences	685:711	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	9	26	theme	chemotaxonomic	1233:1246	arg1	features					1248:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	Moreover, multiple genomic, physiological and chemotaxonomic features supported the discrimination between these three strains.
36191051	7	27	theme	DNA-DNA	948:954	arg1	hybridization					956:968	digital DNA-DNA hybridization	940:968	digital DNA-DNA hybridization	940:968	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	12	28	theme	Anaeromyxobacter	1536:1551	arg1	paludicola					1553:1562	nov. and Anaeromyxobacter paludicola	1527:1562	nov. and Anaeromyxobacter paludicola	1527:1562	nov. and Anaeromyxobacter paludicola sp.
36191051	6	29	theme	Phylogenetic	442:453	arg1	trees					455:459	Phylogenetic trees	442:459	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences	442:522	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	9	30	theme	physiological	1215:1227	arg1	features					1248:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	Moreover, multiple genomic, physiological and chemotaxonomic features supported the discrimination between these three strains.
36191051	2	31	dep	sp	107:108	arg1	paludicola					96:105	nov. and Anaeromyxobacter paludicola	70:105	nov. and Anaeromyxobacter paludicola	70:105	nov. and Anaeromyxobacter paludicola sp.
36191051	4	32	dep	strains	160:166	arg1	Red267T					178:184	Red267T	178:184	Red267T	178:184	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	4	32	dep	strains	160:166	arg1	strains					160:166	Three bacterial strains	144:166	Three bacterial strains (Red232T, Red267T and Red630T)	144:197	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	4	32	dep	strains	160:166	arg1	Red630T					190:196	Red630T	190:196	Red630T	190:196	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	8	33	located	observed	1100:1107	arg2	genes					1089:1093	Nitrogenase genes	1077:1093	Nitrogenase genes	1077:1093	Nitrogenase genes were observed in the genomes of the three novel strains, but not in A. dehalogenans 2CP-1T.
36191051	8	33	located	observed	1100:1107	arg1	genomes					1116:1122	the genomes	1112:1122	the genomes of the three novel strains	1112:1149	Nitrogenase genes were observed in the genomes of the three novel strains, but not in A. dehalogenans 2CP-1T.
36191051	6	34	theme	Anaeromyxobacter	596:611	arg1	species					613:619	Anaeromyxobacter species	596:619	Anaeromyxobacter species	596:619	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	14	35	theme	114074T=MCCC	1609:1620	arg1	Red232T					1594:1600	Red232T	1594:1600	Red232T (=NBRC 114074T=MCCC 1K03954T)	1594:1630	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	14	35	theme	114074T=MCCC	1609:1620	arg1	1K03954T					1622:1629	=NBRC 114074T=MCCC 1K03954T	1603:1629	=NBRC 114074T=MCCC 1K03954T	1603:1629	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	10	36	theme	following	1427:1435	arg1	names					1437:1441	the following names	1423:1441	the following names	1423:1441	Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp.
36191051	0	37	theme	Anaeromyxobacter	0:15	arg1	sp					24:25	Anaeromyxobacter oryzae sp	0:25	Anaeromyxobacter oryzae sp.	0:26	Anaeromyxobacter oryzae sp.
36191051	7	38	theme	Anaeromyxobacter	781:796	arg1	2CP-1T					829:834	Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T	781:834	2CP-1T	829:834	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	7	38	theme	Anaeromyxobacter	781:796	arg1	Anaeromyxobacter					799:814	Anaeromyxobacter	799:814	Anaeromyxobacter	799:814	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	7	39	theme	average	896:902	arg1	identity					915:922	average nucleotide identity	896:922	average nucleotide identity	896:922	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	10	40	theme	oryzae	1474:1479	arg1	sp					1481:1482	Anaeromyxobacter oryzae sp	1457:1482	three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp	1381:1482	Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp.
36191051	6	41	theme	16S	470:472	arg1	rRNA					474:477	16S rRNA	470:477	16S rRNA gene	470:482	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	1	42	theme	diazotrophicus	51:64	arg1	sp					66:67	Anaeromyxobacter diazotrophicus sp	34:67	Anaeromyxobacter diazotrophicus sp	34:67	nov., Anaeromyxobacter diazotrophicus sp.
36191051	1	42	theme	diazotrophicus	51:64	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., Anaeromyxobacter diazotrophicus sp.
36191051	5	43	theme	red	413:415	arg1	colonies					417:424	red colonies	413:424	red colonies on agar plates	413:439	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	12	44	dep	sp	1564:1565	arg1	paludicola					1553:1562	nov. and Anaeromyxobacter paludicola	1527:1562	nov. and Anaeromyxobacter paludicola	1527:1562	nov. and Anaeromyxobacter paludicola sp.
36191051	7	45	theme	rRNA	861:864	arg1	sequence					871:878	16S rRNA gene sequence	857:878	16S rRNA gene sequence	857:878	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	14	46	theme	=NBRC	1685:1689	arg1	1K03957T					1704:1711	=NBRC 114076T=MCCC 1K03957T	1685:1711	=NBRC 114076T=MCCC 1K03957T	1685:1711	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	14	46	theme	=NBRC	1685:1689	arg1	Red630T					1676:1682	Red630T	1676:1682	Red630T (=NBRC 114076T=MCCC 1K03957T)	1676:1712	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	6	47	theme	gene	509:512	arg1	sequences					514:522	multiple single-copy gene sequences	488:522	multiple single-copy gene sequences	488:522	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	7	48	theme	delineation	1053:1063	arg1	thresholds					1065:1074	the species delineation thresholds	1041:1074	the species delineation thresholds	1041:1074	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	11	49	theme	diazotrophicus	1508:1521	arg1	sp					1523:1524	Anaeromyxobacter diazotrophicus sp	1491:1524	Anaeromyxobacter diazotrophicus sp	1491:1524	nov., Anaeromyxobacter diazotrophicus sp.
36191051	11	49	theme	diazotrophicus	1508:1521	arg1	nov.					1485:1488	nov.	1485:1488	nov.	1485:1488	nov., Anaeromyxobacter diazotrophicus sp.
36191051	8	50	theme	strains	1143:1149	arg1	genomes					1116:1122	the genomes	1112:1122	the genomes of the three novel strains	1112:1149	Nitrogenase genes were observed in the genomes of the three novel strains, but not in A. dehalogenans 2CP-1T.
36191051	6	51	from	genera	656:661	arg1	independent					622:632	independent	622:632	independent	622:632	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	6	52	theme	type	580:583	arg1	strains					585:591	the type strains	576:591	the type strains of Anaeromyxobacter species	576:619	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	2	53	theme	nov.	70:73	arg1	paludicola					96:105	nov. and Anaeromyxobacter paludicola	70:105	nov. and Anaeromyxobacter paludicola	70:105	nov. and Anaeromyxobacter paludicola sp.
36191051	7	54	theme	rRNA	693:696	arg1	sequences					703:711	the 16S rRNA gene sequences	685:711	the 16S rRNA gene sequences	685:711	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	7	55	theme	dehalogenans	816:827	arg1	2CP-1T					829:834	Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T	781:834	2CP-1T	829:834	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	7	55	theme	dehalogenans	816:827	arg1	Anaeromyxobacter					799:814	Anaeromyxobacter	799:814	Anaeromyxobacter	799:814	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	4	56	theme	bacterial	150:158	arg1	Red267T					178:184	Red267T	178:184	Red267T	178:184	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	4	56	theme	bacterial	150:158	arg1	strains					160:166	Three bacterial strains	144:166	Three bacterial strains (Red232T, Red267T and Red630T)	144:197	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	4	56	theme	bacterial	150:158	arg1	Red630T					190:196	Red630T	190:196	Red630T	190:196	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	9	57	theme	multiple	1197:1204	arg1	features					1248:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	multiple genomic, physiological and chemotaxonomic features	1197:1255	Moreover, multiple genomic, physiological and chemotaxonomic features supported the discrimination between these three strains.
36191051	7	58	theme	amino	997:1001	arg1	identity					1008:1015	average amino acid identity	989:1015	average amino acid identity	989:1015	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	8	59	theme	dehalogenans	1166:1177	arg1	2CP-1T					1179:1184	A. dehalogenans 2CP-1T	1163:1184	A. dehalogenans 2CP-1T	1163:1184	Nitrogenase genes were observed in the genomes of the three novel strains, but not in A. dehalogenans 2CP-1T.
36191051	5	60	theme	anaerobic	309:317	arg1	structures					377:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures	276:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility	276:399	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	14	61	theme	type	1577:1580	arg1	Red232T					1594:1600	Red232T	1594:1600	Red232T (=NBRC 114074T=MCCC 1K03954T)	1594:1630	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	14	61	theme	type	1577:1580	arg1	strains					1582:1588	The type strains	1573:1588	The type strains	1573:1588	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	6	62	theme	strain	649:654	arg1	genera					656:661	any other strain genera	639:661	any other strain genera	639:661	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	2	63	theme	Anaeromyxobacter	79:94	arg1	paludicola					96:105	nov. and Anaeromyxobacter paludicola	70:105	nov. and Anaeromyxobacter paludicola	70:105	nov. and Anaeromyxobacter paludicola sp.
36191051	7	64	theme	acid	1003:1006	arg1	identity					1008:1015	average amino acid identity	989:1015	average amino acid identity	989:1015	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	14	65	theme	114075T=MCCC	1648:1659	arg1	1K04211T					1661:1668	=NBRC 114075T=MCCC 1K04211T	1642:1668	=NBRC 114075T=MCCC 1K04211T	1642:1668	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	14	65	theme	114075T=MCCC	1648:1659	arg1	Red267T					1633:1639	Red267T	1633:1639	Red267T (=NBRC 114075T=MCCC 1K04211T)	1633:1669	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	4	66	theme	paddy	218:222	arg1	soils					224:228	paddy soils	218:228	paddy soils sampled in Japan	218:245	Three bacterial strains (Red232T, Red267T and Red630T) were isolated from paddy soils sampled in Japan.
36191051	3	67	theme	paddy	131:135	arg1	soils					137:141	paddy soils	131:141	paddy soils	131:141	nov., isolated from paddy soils.
36191051	6	68	theme	species	613:619	arg1	strains					585:591	the type strains	576:591	the type strains of Anaeromyxobacter species	576:619	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	7	69	theme	digital	940:946	arg1	hybridization					956:968	digital DNA-DNA hybridization	940:968	digital DNA-DNA hybridization	940:968	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	5	70	from	colonies	417:424	arg1	plates					434:439	agar plates	429:439	agar plates	429:439	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	5	71	theme	pilus-like	366:375	arg1	structures					377:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures	276:386	Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility	276:399	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	10	72	theme	independent	1393:1403	arg1	species					1405:1411	three novel independent species	1381:1411	three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp	1381:1482	Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp.
36191051	14	73	theme	=NBRC	1603:1607	arg1	Red232T					1594:1600	Red232T	1594:1600	Red232T (=NBRC 114074T=MCCC 1K03954T)	1594:1630	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	14	73	theme	=NBRC	1603:1607	arg1	1K03954T					1622:1629	=NBRC 114074T=MCCC 1K03954T	1603:1629	=NBRC 114074T=MCCC 1K03954T	1603:1629	The type strains are Red232T (=NBRC 114074T=MCCC 1K03954T), Red267T (=NBRC 114075T=MCCC 1K04211T), and Red630T (=NBRC 114076T=MCCC 1K03957T), respectively.
36191051	0	74	theme	oryzae	17:22	arg1	sp					24:25	Anaeromyxobacter oryzae sp	0:25	Anaeromyxobacter oryzae sp.	0:26	Anaeromyxobacter oryzae sp.
36191051	5	75	with	rod-shaped	325:334	arg1	flagella					354:361	monotrichous flagella	341:361	monotrichous flagella	341:361	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	10	76	from	evidence	1328:1335	arg1	study					1345:1349	this study	1340:1349	this study	1340:1349	Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp.
36191051	10	77	theme	Anaeromyxobacter	1457:1472	arg1	sp					1481:1482	Anaeromyxobacter oryzae sp	1457:1482	three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp	1381:1482	Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp.
36191051	10	78	dep	species	1405:1411	arg1	sp					1481:1482	Anaeromyxobacter oryzae sp	1457:1482	three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp	1381:1482	Based on the evidence in this study, the three isolates represent three novel independent species for which the following names are proposed: Anaeromyxobacter oryzae sp.
36191051	6	79	theme	rRNA	474:477	arg1	gene					479:482	16S rRNA gene	470:482	16S rRNA gene	470:482	Phylogenetic trees based on 16S rRNA gene and multiple single-copy gene sequences showed that the three strains formed a cluster with the type strains of Anaeromyxobacter species, independent from any other strain genera.
36191051	7	80	theme	type	766:769	arg1	strain					771:776	the type strain	762:776	the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T	762:834	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	8	81	theme	Nitrogenase	1077:1087	arg1	genes					1089:1093	Nitrogenase genes	1077:1093	Nitrogenase genes	1077:1093	Nitrogenase genes were observed in the genomes of the three novel strains, but not in A. dehalogenans 2CP-1T.
36191051	5	82	theme	agar	429:432	arg1	plates					434:439	agar plates	429:439	agar plates	429:439	Cells of these strains were Gram-stain-negative, facultative anaerobic, long rod-shaped with monotrichous flagella or pilus-like structures for motility, and formed red colonies on agar plates.
36191051	7	83	theme	isolated	741:748	arg1	strains					750:756	the three isolated strains	731:756	the three isolated strains	731:756	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
36191051	1	84	theme	Anaeromyxobacter	34:49	arg1	sp					66:67	Anaeromyxobacter diazotrophicus sp	34:67	Anaeromyxobacter diazotrophicus sp	34:67	nov., Anaeromyxobacter diazotrophicus sp.
36191051	1	84	theme	Anaeromyxobacter	34:49	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., Anaeromyxobacter diazotrophicus sp.
36191051	7	85	theme	gene	866:869	arg1	sequence					871:878	16S rRNA gene sequence	857:878	16S rRNA gene sequence	857:878	Similarity values of the 16S rRNA gene sequences and genomes among the three isolated strains and the type strain of Anaeromyxobacter, Anaeromyxobacter dehalogenans 2CP-1T, were 95.4-97.4% for 16S rRNA gene sequence, 75.3-79.5% for average nucleotide identity, 19.6-21.7% for digital DNA-DNA hybridization and 64.1-72.6% for average amino acid identity, all of which are below the species delineation thresholds.
35403732	8	0	theme	Hdc	1120:1122	arg1	expression					1124:1133	Hdc expression	1120:1133	Hdc expression in PCa	1120:1140	Intraperitoneal injection of LPS increased Hdc expression in PCa.
35403732	6	1	theme	suppressor	920:929	arg1	cells					931:935	myeloid-derived suppressor cells	904:935	myeloid-derived suppressor cells	904:935	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	11	2	theme	histamine	1395:1403	arg1	signaling					1405:1413	histamine signaling	1395:1413	histamine signaling via mast cells	1395:1428	In conclusion, HFD promotes PCa growth through histamine signaling via mast cells.
35403732	10	3	theme	patients	1338:1345	arg1	specimens					1310:1318	total prostatectomy specimens	1290:1318	total prostatectomy specimens of severely obese patients	1290:1345	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	10	4	theme	mast	1243:1246	arg1	cells					1248:1252	mast cells	1243:1252	mast cells	1243:1252	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	5	5	theme	mast	726:729	arg1	cells					731:735	mast cells	726:735	mast cells	726:735	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	4	6	theme	inflammatory	486:497	arg1	growth					506:511	HFD-induced inflammatory cancer growth	474:511	HFD-induced inflammatory cancer growth	474:511	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	11	7	theme	PCa	1376:1378	arg1	growth					1380:1385	PCa growth	1376:1385	PCa growth	1376:1385	In conclusion, HFD promotes PCa growth through histamine signaling via mast cells.
35403732	12	8	theme	inflammatory	1495:1506	arg1	growth					1515:1520	the inflammatory cancer growth	1491:1520	the inflammatory cancer growth	1491:1520	Dietary high-fat induced gut dysbiosis might be involved in the inflammatory cancer growth.
35403732	4	9	theme	microbiome	544:553	arg1	involvement					521:531	the involvement	517:531	the involvement of the gut microbiome	517:553	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	4	9	theme	microbiome	544:553	arg1	etiology					462:469	the etiology	458:469	the etiology of HFD-induced inflammatory cancer growth	458:511	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	3	10	theme	microbiota	319:328	arg1	changes					330:336	the gut microbiota changes	311:336	the gut microbiota changes under the influence of diet	311:364	The composition of the gut microbiota changes under the influence of diet exert various effects on the host through immunological mechanisms.
35403732	4	11	theme	HFD-induced	474:484	arg1	growth					506:511	HFD-induced inflammatory cancer growth	474:511	HFD-induced inflammatory cancer growth	474:511	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	7	12	theme	levels	1069:1074	arg1	elevation					1025:1033	the elevation	1021:1033	the elevation of serum lipopolysaccharide (LPS) levels	1021:1074	HFD intake induced gut dysbiosis, resulting in the elevation of serum lipopolysaccharide (LPS) levels.
35403732	5	13	theme	Hdc	574:576	arg1	expression					560:569	The expression	556:569	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels	556:647	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	6	14	theme	IL6/STAT3	953:961	arg1	signaling					963:971	IL6/STAT3 signaling	953:971	IL6/STAT3 signaling	953:971	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	3	15	theme	changes	330:336	arg1	composition					296:306	The composition	292:306	The composition of the gut microbiota changes under the influence of diet	292:364	The composition of the gut microbiota changes under the influence of diet exert various effects on the host through immunological mechanisms.
35403732	5	16	theme	histamine	632:640	arg1	levels					642:647	histamine levels	632:647	histamine levels	632:647	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	9	17	theme	LPS/Toll-like	1157:1169	arg1	signaling					1182:1190	LPS/Toll-like receptor 4 signaling	1157:1190	LPS/Toll-like receptor 4 signaling	1157:1190	Inhibition of LPS/Toll-like receptor 4 signaling suppressed HFD-induced tumor growth.
35403732	10	18	theme	cancer	1275:1280	arg1	foci					1282:1285	the cancer foci	1271:1285	the cancer foci in total prostatectomy specimens of severely obese patients	1271:1345	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	6	19	theme	tumor	849:853	arg1	growth					855:860	tumor growth	849:860	tumor growth	849:860	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	5	20	theme	gene	583:586	arg1	expression					560:569	The expression	556:569	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels	556:647	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	9	21	theme	receptor	1171:1178	arg1	signaling					1182:1190	LPS/Toll-like receptor 4 signaling	1157:1190	LPS/Toll-like receptor 4 signaling	1157:1190	Inhibition of LPS/Toll-like receptor 4 signaling suppressed HFD-induced tumor growth.
35403732	1	22	theme	Western	75:81	arg1	factor					133:138	a major risk factor	120:138	a major risk factor for prostate cancer (PCa)	120:164	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	22	theme	Western	75:81	arg1	HFD					99:101	HFD	99:101	HFD	99:101	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	22	theme	Western	75:81	arg1	diets					92:96	Western high-fat diets	75:96	Western high-fat diets (HFD)	75:102	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	23	theme	prostate	144:151	arg1	PCa					161:163	PCa	161:163	PCa	161:163	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	23	theme	prostate	144:151	arg1	cancer					153:158	prostate cancer	144:158	prostate cancer (PCa)	144:164	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	3	24	theme	gut	315:317	arg1	changes					330:336	the gut microbiota changes	311:336	the gut microbiota changes under the influence of diet	311:364	The composition of the gut microbiota changes under the influence of diet exert various effects on the host through immunological mechanisms.
35403732	5	25	theme	cells	731:735	arg1	number					716:721	the number	712:721	the number of mast cells	712:735	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	7	26	theme	serum	1038:1042	arg1	LPS					1064:1066	LPS	1064:1066	LPS	1064:1066	HFD intake induced gut dysbiosis, resulting in the elevation of serum lipopolysaccharide (LPS) levels.
35403732	7	26	theme	serum	1038:1042	arg1	lipopolysaccharide					1044:1061	serum lipopolysaccharide	1038:1061	serum lipopolysaccharide (LPS) levels	1038:1074	HFD intake induced gut dysbiosis, resulting in the elevation of serum lipopolysaccharide (LPS) levels.
35403732	1	27	theme	high-fat	83:90	arg1	factor					133:138	a major risk factor	120:138	a major risk factor for prostate cancer (PCa)	120:164	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	27	theme	high-fat	83:90	arg1	HFD					99:101	HFD	99:101	HFD	99:101	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	27	theme	high-fat	83:90	arg1	diets					92:96	Western high-fat diets	75:96	Western high-fat diets (HFD)	75:102	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	5	28	theme	tumor	758:762	arg1	foci					764:767	the tumor foci	754:767	the tumor foci	754:767	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	7	29	theme	lipopolysaccharide	1044:1061	arg1	levels					1069:1074	serum lipopolysaccharide (LPS) levels	1038:1074	serum lipopolysaccharide (LPS) levels	1038:1074	HFD intake induced gut dysbiosis, resulting in the elevation of serum lipopolysaccharide (LPS) levels.
35403732	5	30	theme	responsible	588:598	arg1	gene					583:586	the gene	579:586	the gene responsible for histamine biosynthesis	579:625	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	6	31	theme	HFD-fed	865:871	arg1	mice					873:876	HFD-fed mice	865:876	HFD-fed mice	865:876	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	11	32	theme	mast	1419:1422	arg1	cells					1424:1428	mast cells	1419:1428	mast cells	1419:1428	In conclusion, HFD promotes PCa growth through histamine signaling via mast cells.
35403732	0	33	theme	High-fat	0:7	arg1	diet					9:12	High-fat diet	0:12	High-fat diet	0:12	High-fat diet promotes prostate cancer growth through histamine signaling.
35403732	5	34	theme	histamine	604:612	arg1	biosynthesis					614:625	histamine biosynthesis	604:625	histamine biosynthesis	604:625	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	12	35	theme	Dietary	1431:1437	arg1	dysbiosis					1460:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis might be involved in the inflammatory cancer growth.
35403732	2	36	theme	PCa	280:282	arg1	growth					284:289	inflammatory PCa growth	267:289	inflammatory PCa growth	267:289	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	5	37	theme	HFD-fed	694:700	arg1	mice					702:705	HFD-fed mice	694:705	HFD-fed mice	694:705	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	0	38	theme	prostate	23:30	arg1	growth					39:44	prostate cancer growth	23:44	prostate cancer growth	23:44	High-fat diet promotes prostate cancer growth through histamine signaling.
35403732	6	39	theme	cells	931:935	arg1	number					894:899	the number	890:899	the number of myeloid-derived suppressor cells	890:935	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	5	40	theme	large	669:673	arg1	tumors					684:689	large prostate tumors	669:689	large prostate tumors of HFD-fed mice	669:705	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	9	41	theme	HFD-induced	1203:1213	arg1	growth					1221:1226	HFD-induced tumor growth	1203:1226	HFD-induced tumor growth	1203:1226	Inhibition of LPS/Toll-like receptor 4 signaling suppressed HFD-induced tumor growth.
35403732	2	42	theme	inflammatory	267:278	arg1	growth					284:289	inflammatory PCa growth	267:289	inflammatory PCa growth	267:289	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	10	43	theme	total	1290:1294	arg1	specimens					1310:1318	total prostatectomy specimens	1290:1318	total prostatectomy specimens of severely obese patients	1290:1345	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	4	44	theme	gut	540:542	arg1	microbiome					544:553	the gut microbiome	536:553	the gut microbiome	536:553	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	5	45	theme	prostate	675:682	arg1	tumors					684:689	large prostate tumors	669:689	large prostate tumors of HFD-fed mice	669:705	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	9	46	theme	tumor	1215:1219	arg1	growth					1221:1226	HFD-induced tumor growth	1203:1226	HFD-induced tumor growth	1203:1226	Inhibition of LPS/Toll-like receptor 4 signaling suppressed HFD-induced tumor growth.
35403732	10	47	theme	prostatectomy	1296:1308	arg1	specimens					1310:1318	total prostatectomy specimens	1290:1318	total prostatectomy specimens of severely obese patients	1290:1345	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	3	48	theme	various	372:378	arg1	effects					380:386	various effects	372:386	various effects	372:386	The composition of the gut microbiota changes under the influence of diet exert various effects on the host through immunological mechanisms.
35403732	6	49	theme	myeloid-derived	904:918	arg1	cells					931:935	myeloid-derived suppressor cells	904:935	myeloid-derived suppressor cells	904:935	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	6	50	theme	receptor	817:824	arg1	fexofenadine					788:799	fexofenadine	788:799	fexofenadine	788:799	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	6	50	theme	receptor	817:824	arg1	antagonist					826:835	a histamine H1 receptor antagonist	802:835	a histamine H1 receptor antagonist	802:835	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	3	51	theme	immunological	408:420	arg1	mechanisms					422:431	immunological mechanisms	408:431	immunological mechanisms	408:431	The composition of the gut microbiota changes under the influence of diet exert various effects on the host through immunological mechanisms.
35403732	0	52	theme	cancer	32:37	arg1	growth					39:44	prostate cancer growth	23:44	prostate cancer growth	23:44	High-fat diet promotes prostate cancer growth through histamine signaling.
35403732	4	53	theme	growth	506:511	arg1	involvement					521:531	the involvement	517:531	the involvement of the gut microbiome	517:553	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	4	53	theme	growth	506:511	arg1	etiology					462:469	the etiology	458:469	the etiology of HFD-induced inflammatory cancer growth	458:511	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	6	54	theme	H1	814:815	arg1	fexofenadine					788:799	fexofenadine	788:799	fexofenadine	788:799	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	6	54	theme	H1	814:815	arg1	antagonist					826:835	a histamine H1 receptor antagonist	802:835	a histamine H1 receptor antagonist	802:835	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	6	55	link	myeloid-derived	904:918	arg1	cells					931:935	myeloid-derived suppressor cells	904:935	myeloid-derived suppressor cells	904:935	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	10	56	theme	obese	1332:1336	arg1	patients					1338:1345	severely obese patients	1323:1345	severely obese patients	1323:1345	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	0	57	theme	histamine	54:62	arg1	signaling					64:72	histamine signaling	54:72	histamine signaling	54:72	High-fat diet promotes prostate cancer growth through histamine signaling.
35403732	4	58	theme	cancer	499:504	arg1	growth					506:511	HFD-induced inflammatory cancer growth	474:511	HFD-induced inflammatory cancer growth	474:511	Herein, we investigated the etiology of HFD-induced inflammatory cancer growth and the involvement of the gut microbiome.
35403732	5	59	theme	levels	642:647	arg1	expression					560:569	The expression	556:569	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels	556:647	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35403732	6	60	theme	histamine	804:812	arg1	fexofenadine					788:799	fexofenadine	788:799	fexofenadine	788:799	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	6	60	theme	histamine	804:812	arg1	antagonist					826:835	a histamine H1 receptor antagonist	802:835	a histamine H1 receptor antagonist	802:835	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	7	61	theme	gut	993:995	arg1	dysbiosis					997:1005	gut dysbiosis	993:1005	gut dysbiosis	993:1005	HFD intake induced gut dysbiosis, resulting in the elevation of serum lipopolysaccharide (LPS) levels.
35403732	12	62	theme	induced	1448:1454	arg1	dysbiosis					1460:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis might be involved in the inflammatory cancer growth.
35403732	2	63	theme	prostate-specific	173:189	arg1	model					219:223	a PCa model	213:223	a PCa model	213:223	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	2	63	theme	prostate-specific	173:189	arg1	mice					205:208	prostate-specific Pten-knockout mice	173:208	prostate-specific Pten-knockout mice	173:208	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	2	64	theme	Pten-knockout	191:203	arg1	model					219:223	a PCa model	213:223	a PCa model	213:223	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	2	64	theme	Pten-knockout	191:203	arg1	mice					205:208	prostate-specific Pten-knockout mice	173:208	prostate-specific Pten-knockout mice	173:208	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	7	65	theme	HFD	974:976	arg1	intake					978:983	HFD intake	974:983	HFD intake	974:983	HFD intake induced gut dysbiosis, resulting in the elevation of serum lipopolysaccharide (LPS) levels.
35403732	12	66	theme	cancer	1508:1513	arg1	growth					1515:1520	the inflammatory cancer growth	1491:1520	the inflammatory cancer growth	1491:1520	Dietary high-fat induced gut dysbiosis might be involved in the inflammatory cancer growth.
35403732	12	67	theme	gut	1456:1458	arg1	dysbiosis					1460:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis might be involved in the inflammatory cancer growth.
35403732	8	68	theme	Intraperitoneal	1077:1091	arg1	injection					1093:1101	Intraperitoneal injection	1077:1101	Intraperitoneal injection of LPS	1077:1108	Intraperitoneal injection of LPS increased Hdc expression in PCa.
35403732	6	69	theme	fexofenadine	788:799	arg1	Administration					770:783	Administration	770:783	Administration of fexofenadine, a histamine H1 receptor antagonist,	770:836	Administration of fexofenadine, a histamine H1 receptor antagonist, suppressed tumor growth in HFD-fed mice by reducing the number of myeloid-derived suppressor cells and suppressing IL6/STAT3 signaling.
35403732	10	70	from	foci	1282:1285	arg1	specimens					1310:1318	total prostatectomy specimens	1290:1318	total prostatectomy specimens of severely obese patients	1290:1345	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	8	71	theme	LPS	1106:1108	arg1	injection					1093:1101	Intraperitoneal injection	1077:1101	Intraperitoneal injection of LPS	1077:1108	Intraperitoneal injection of LPS increased Hdc expression in PCa.
35403732	8	72	from	expression	1124:1133	arg1	PCa					1138:1140	PCa	1138:1140	PCa	1138:1140	Intraperitoneal injection of LPS increased Hdc expression in PCa.
35403732	1	73	theme	major	122:126	arg1	factor					133:138	a major risk factor	120:138	a major risk factor for prostate cancer (PCa)	120:164	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	73	theme	major	122:126	arg1	diets					92:96	Western high-fat diets	75:96	Western high-fat diets (HFD)	75:102	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	9	74	theme	signaling	1182:1190	arg1	Inhibition					1143:1152	Inhibition	1143:1152	Inhibition of LPS/Toll-like receptor 4 signaling	1143:1190	Inhibition of LPS/Toll-like receptor 4 signaling suppressed HFD-induced tumor growth.
35403732	3	75	theme	diet	361:364	arg1	influence					348:356	the influence	344:356	the influence of diet	344:364	The composition of the gut microbiota changes under the influence of diet exert various effects on the host through immunological mechanisms.
35403732	12	76	theme	high-fat	1439:1446	arg1	dysbiosis					1460:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis	1431:1468	Dietary high-fat induced gut dysbiosis might be involved in the inflammatory cancer growth.
35403732	2	77	theme	PCa	215:217	arg1	model					219:223	a PCa model	213:223	a PCa model	213:223	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	2	77	theme	PCa	215:217	arg1	mice					205:208	prostate-specific Pten-knockout mice	173:208	prostate-specific Pten-knockout mice	173:208	Using prostate-specific Pten-knockout mice as a PCa model, we previously reported that HFD promoted inflammatory PCa growth.
35403732	1	78	theme	risk	128:131	arg1	factor					133:138	a major risk factor	120:138	a major risk factor for prostate cancer (PCa)	120:164	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	1	78	theme	risk	128:131	arg1	diets					92:96	Western high-fat diets	75:96	Western high-fat diets (HFD)	75:102	Western high-fat diets (HFD) are regarded as a major risk factor for prostate cancer (PCa).
35403732	10	79	theme	cells	1248:1252	arg1	number					1233:1238	The number	1229:1238	The number of mast cells	1229:1252	The number of mast cells increased around the cancer foci in total prostatectomy specimens of severely obese patients.
35403732	5	80	theme	mice	702:705	arg1	tumors					684:689	large prostate tumors	669:689	large prostate tumors of HFD-fed mice	669:705	The expression of Hdc, the gene responsible for histamine biosynthesis, and histamine levels were upregulated in large prostate tumors of HFD-fed mice, and the number of mast cells increased around the tumor foci.
35194841	8	0	theme	extended	1483:1490	arg1	structures					1492:1501	extended structures	1483:1501	extended structures that sense carbohydrates	1483:1526	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	3	1	theme	membrane-embedded	556:572	arg1	RsgI1-9					590:596	RsgI1-9	590:596	RsgI1-9	590:596	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	3	1	theme	membrane-embedded	556:572	arg1	factors					581:587	nine membrane-embedded anti-σ factors	551:587	nine membrane-embedded anti-σ factors (RsgI1-9)	551:597	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	6	2	theme	ectodomain	1071:1080	arg1	experiments					1045:1055	Small-angle X-ray scattering experiments	1016:1055	Small-angle X-ray scattering experiments of the intact ectodomain	1016:1080	Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation.
35194841	7	3	theme	CRE	1192:1194	arg1	domain					1197:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	3	4	theme	anti-σ	574:579	arg1	RsgI1-9					590:596	RsgI1-9	590:596	RsgI1-9	590:596	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	3	4	theme	anti-σ	574:579	arg1	factors					581:587	nine membrane-embedded anti-σ factors	551:587	nine membrane-embedded anti-σ factors (RsgI1-9)	551:597	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	6	5	theme	intact	1064:1069	arg1	ectodomain					1071:1080	the intact ectodomain	1060:1080	the intact ectodomain	1060:1080	Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation.
35194841	7	6	theme	RsgI	1172:1175	arg1	domain					1197:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	5	7	theme	protein	947:953	arg1	domains					955:961	NTF2-like protein domains	937:961	NTF2-like protein domains that contain a potential binding site for cellulose	937:1013	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	7	8	theme	proline-rich	1240:1251	arg1	linker					1253:1258	a proline-rich linker	1238:1258	a proline-rich linker	1238:1258	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	7	9	theme	extracellular	1177:1189	arg1	domain					1197:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	2	10	theme	bacterium	290:298	arg1	ability					307:313	its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes	303:467	its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes	303:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	2	10	theme	bacterium	290:298	arg1	feature					274:280	An attractive feature	260:280	An attractive feature of this bacterium	260:298	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	4	11	theme	RsgI9	773:777	arg1	factor					786:791	the RsgI9 anti-σ factor	769:791	the RsgI9 anti-σ factor	769:791	Here we show that the RsgI9 anti-σ factor interacts with cellulose via a C-terminal bi-domain unit.
35194841	0	12	theme	sensing	110:116	arg1	mechanism					89:97	the mechanism	85:97	the mechanism of biomass sensing	85:116	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.
35194841	5	13	theme	S1C	919:921	arg1	peptidase					923:931	S1C peptidase	919:931	S1C peptidase	919:931	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	4	14	theme	bi-domain	835:843	arg1	unit					845:848	a C-terminal bi-domain unit	822:848	a C-terminal bi-domain unit	822:848	Here we show that the RsgI9 anti-σ factor interacts with cellulose via a C-terminal bi-domain unit.
35194841	5	15	theme	potential	978:986	arg1	site					996:999	a potential binding site	976:999	a potential binding site for cellulose	976:1013	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	2	16	theme	different	432:440	arg1	types					442:446	house different types	426:446	house different types of cellulase enzymes	426:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	0	17	theme	biomass	102:108	arg1	sensing					110:116	biomass sensing	102:116	biomass sensing	102:116	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.
35194841	7	18	theme	conserved	1162:1170	arg1	domain					1197:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	a conserved RsgI extracellular (CRE) domain	1160:1202	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	6	19	dep	bi-lobed	1108:1115	arg1	elongated					1118:1126	elongated	1118:1126	elongated	1118:1126	Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation.
35194841	4	20	theme	C-terminal	824:833	arg1	unit					845:848	a C-terminal bi-domain unit	822:848	a C-terminal bi-domain unit	822:848	Here we show that the RsgI9 anti-σ factor interacts with cellulose via a C-terminal bi-domain unit.
35194841	1	21	theme	microbial	177:185	arg1	platform					187:194	a microbial platform	175:194	a microbial platform to produce biofuels and chemicals from renewable plant biomass	175:257	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	1	21	theme	microbial	177:185	arg1	thermocellum					131:142	Clostridium thermocellum	119:142	Clostridium thermocellum	119:142	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	5	22	theme	crystal	859:865	arg1	structure					867:875	A 2.0 Å crystal structure	851:875	A 2.0 Å crystal structure	851:875	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	3	23	theme	sense	621:625	arg1	types					637:641	sense different types	621:641	sense different types of extracellular carbohydrates	621:672	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	3	24	theme	Clostridium	470:480	arg1	thermocellum					482:493	Clostridium thermocellum	470:493	Clostridium thermocellum	470:493	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	6	25	theme	scattering	1034:1043	arg1	experiments					1045:1055	Small-angle X-ray scattering experiments	1016:1055	Small-angle X-ray scattering experiments of the intact ectodomain	1016:1080	Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation.
35194841	8	26	theme	sense	1508:1512	arg1	carbohydrates					1514:1526	sense carbohydrates	1508:1526	sense carbohydrates	1508:1526	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	5	27	theme	binding	988:994	arg1	site					996:999	a potential binding site	976:999	a potential binding site for cellulose	976:1013	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	2	28	theme	enzymes	461:467	arg1	types					442:446	house different types	426:446	house different types of cellulase enzymes	426:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	8	29	theme	C.	1415:1416	arg1	factors					1438:1444	several other C. thermocellum anti-σ factors	1401:1444	several other C. thermocellum anti-σ factors	1401:1444	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	6	30	theme	X-ray	1028:1032	arg1	experiments					1045:1055	Small-angle X-ray scattering experiments	1016:1055	Small-angle X-ray scattering experiments of the intact ectodomain	1016:1080	Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation.
35194841	8	31	theme	other	1409:1413	arg1	factors					1438:1444	several other C. thermocellum anti-σ factors	1401:1444	several other C. thermocellum anti-σ factors	1401:1444	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	3	32	theme	expression	704:713	arg1	programs					715:722	distinct gene expression programs	690:722	distinct gene expression programs	690:722	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	8	33	theme	anti-σ	1431:1436	arg1	factors					1438:1444	several other C. thermocellum anti-σ factors	1401:1444	several other C. thermocellum anti-σ factors	1401:1444	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	2	34	theme	cellulase	451:459	arg1	enzymes					461:467	cellulase enzymes	451:467	cellulase enzymes	451:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	6	35	theme	Small-angle	1016:1026	arg1	experiments					1045:1055	Small-angle X-ray scattering experiments	1016:1055	Small-angle X-ray scattering experiments of the intact ectodomain	1016:1080	Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation.
35194841	7	36	theme	carbohydrate-binding	1294:1313	arg1	~160 Å					1320:1325	the carbohydrate-binding unit ~160 Å	1290:1325	the carbohydrate-binding unit ~160 Å from the cell surface	1290:1347	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	8	37	theme	proline-rich	1362:1373	arg1	elements					1375:1382	proline-rich elements	1362:1382	proline-rich elements	1362:1382	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	8	38	dep	C.	1415:1416	arg1	thermocellum					1418:1429	thermocellum	1418:1429	thermocellum	1418:1429	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	6	39	theme	bi-lobed	1108:1115	arg1	conformation					1128:1139	a bi-lobed, elongated conformation	1106:1139	a bi-lobed, elongated conformation	1106:1139	Small-angle X-ray scattering experiments of the intact ectodomain indicate that it adopts a bi-lobed, elongated conformation.
35194841	0	40	theme	Clostridium	21:31	arg1	ectodomain					52:61	the Clostridium thermocellum RsgI9 ectodomain	17:61	the Clostridium thermocellum RsgI9 ectodomain	17:61	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.
35194841	3	41	theme	distinct	690:697	arg1	programs					715:722	distinct gene expression programs	690:722	distinct gene expression programs	690:722	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	3	42	theme	extracellular	646:658	arg1	carbohydrates					660:672	extracellular carbohydrates	646:672	extracellular carbohydrates	646:672	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	7	43	from	surface	1341:1347	arg1	~160 Å					1320:1325	the carbohydrate-binding unit ~160 Å	1290:1325	the carbohydrate-binding unit ~160 Å from the cell surface	1290:1347	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	3	44	theme	enzyme	507:512	arg1	composition					514:524	the enzyme composition	503:524	the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors	503:748	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	0	45	theme	ectodomain	52:61	arg1	structure					4:12	The structure	0:12	The structure of the Clostridium thermocellum RsgI9 ectodomain	0:61	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.
35194841	5	46	theme	NTF2-like	937:945	arg1	domains					955:961	NTF2-like protein domains	937:961	NTF2-like protein domains that contain a potential binding site for cellulose	937:1013	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	5	47	theme	2.0 Å	853:857	arg1	structure					867:875	A 2.0 Å crystal structure	851:875	A 2.0 Å crystal structure	851:875	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	3	48	theme	gene	699:702	arg1	programs					715:722	distinct gene expression programs	690:722	distinct gene expression programs	690:722	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	3	49	theme	different	627:635	arg1	types					637:641	sense different types	621:641	sense different types of extracellular carbohydrates	621:672	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	2	50	theme	surface-displayed	359:375	arg1	complexes					411:419	large multi-protein complexes	391:419	large multi-protein complexes that house different types of cellulase enzymes	391:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	2	50	theme	surface-displayed	359:375	arg1	cellulosomes					377:388	surface-displayed cellulosomes	359:388	surface-displayed cellulosomes	359:388	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	0	51	theme	RsgI9	46:50	arg1	ectodomain					52:61	the Clostridium thermocellum RsgI9 ectodomain	17:61	the Clostridium thermocellum RsgI9 ectodomain	17:61	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.
35194841	1	52	from	biomass	251:257	arg1	biofuels					207:214	biofuels	207:214	biofuels	207:214	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	1	52	from	biomass	251:257	arg1	chemicals					220:228	chemicals	220:228	chemicals	220:228	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	3	53	theme	σ	740:740	arg1	factors					742:748	cytoplasmic σ factors	728:748	cytoplasmic σ factors	728:748	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	0	54	dep	Clostridium	21:31	arg1	thermocellum					33:44	thermocellum	33:44	thermocellum	33:44	The structure of the Clostridium thermocellum RsgI9 ectodomain provides insight into the mechanism of biomass sensing.
35194841	1	55	theme	Clostridium	119:129	arg1	thermocellum					131:142	Clostridium thermocellum	119:142	Clostridium thermocellum	119:142	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	1	55	theme	Clostridium	119:129	arg1	platform					187:194	a microbial platform	175:194	a microbial platform to produce biofuels and chemicals from renewable plant biomass	175:257	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	7	56	theme	unit	1315:1318	arg1	~160 Å					1320:1325	the carbohydrate-binding unit ~160 Å	1290:1325	the carbohydrate-binding unit ~160 Å from the cell surface	1290:1347	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	5	57	contain	contain	968:974	arg1	domains					955:961	NTF2-like protein domains	937:961	NTF2-like protein domains that contain a potential binding site for cellulose	937:1013	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	5	57	contain	contain	968:974	arg2	site					996:999	a potential binding site	976:999	a potential binding site for cellulose	976:1013	A 2.0 Å crystal structure reveals that the unit is constructed from S1C peptidase and NTF2-like protein domains that contain a potential binding site for cellulose.
35194841	4	58	theme	anti-σ	779:784	arg1	factor					786:791	the RsgI9 anti-σ factor	769:791	the RsgI9 anti-σ factor	769:791	Here we show that the RsgI9 anti-σ factor interacts with cellulose via a C-terminal bi-domain unit.
35194841	8	59	theme	several	1401:1407	arg1	factors					1438:1444	several other C. thermocellum anti-σ factors	1401:1444	several other C. thermocellum anti-σ factors	1401:1444	The CRE and proline-rich elements are conserved in several other C. thermocellum anti-σ factors, suggesting that they will also form extended structures that sense carbohydrates.
35194841	2	60	theme	attractive	263:272	arg1	ability					307:313	its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes	303:467	its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes	303:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	2	60	theme	attractive	263:272	arg1	feature					274:280	An attractive feature	260:280	An attractive feature of this bacterium	260:298	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	3	61	theme	cellulosome	533:543	arg1	composition					514:524	the enzyme composition	503:524	the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors	503:748	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	1	62	theme	renewable	235:243	arg1	biomass					251:257	renewable plant biomass	235:257	renewable plant biomass	235:257	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	3	63	theme	cytoplasmic	728:738	arg1	factors					742:748	cytoplasmic σ factors	728:748	cytoplasmic σ factors	728:748	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	3	64	theme	carbohydrates	660:672	arg1	types					637:641	sense different types	621:641	sense different types of extracellular carbohydrates	621:672	Clostridium thermocellum tailors the enzyme composition of its cellulosome using nine membrane-embedded anti-σ factors (RsgI1-9), which are thought to sense different types of extracellular carbohydrates and then elicit distinct gene expression programs via cytoplasmic σ factors.
35194841	2	65	theme	multi-protein	397:409	arg1	complexes					411:419	large multi-protein complexes	391:419	large multi-protein complexes that house different types of cellulase enzymes	391:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	2	65	theme	multi-protein	397:409	arg1	cellulosomes					377:388	surface-displayed cellulosomes	359:388	surface-displayed cellulosomes	359:388	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	2	66	theme	house	426:430	arg1	types					442:446	house different types	426:446	house different types of cellulase enzymes	426:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	1	67	theme	plant	245:249	arg1	biomass					251:257	renewable plant biomass	235:257	renewable plant biomass	235:257	Clostridium thermocellum is actively being developed as a microbial platform to produce biofuels and chemicals from renewable plant biomass.
35194841	7	68	theme	cell	1336:1339	arg1	surface					1341:1347	the cell surface	1332:1347	the cell surface	1332:1347	In the structure, a conserved RsgI extracellular (CRE) domain is connected to the bi-domain via a proline-rich linker, which is expected to project the carbohydrate-binding unit ~160 Å from the cell surface.
35194841	2	69	theme	large	391:395	arg1	complexes					411:419	large multi-protein complexes	391:419	large multi-protein complexes that house different types of cellulase enzymes	391:467	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35194841	2	69	theme	large	391:395	arg1	cellulosomes					377:388	surface-displayed cellulosomes	359:388	surface-displayed cellulosomes	359:388	An attractive feature of this bacterium is its ability to efficiently degrade lignocellulose using surface-displayed cellulosomes, large multi-protein complexes that house different types of cellulase enzymes.
35055177	9	0	theme	microbiota	1306:1315	arg1	composition					1317:1327	the intestinal microbiota composition	1291:1327	the intestinal microbiota composition	1291:1327	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	5	1	theme	red	630:632	arg1	O					634:634	oil red O	626:634	oil red O	626:634	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	7	2	theme	fat-diet	974:981	arg1	group					983:987	the fat-diet group	970:987	the fat-diet group	970:987	We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia&nbsp;muciniphila increased by 5-fold with inulin supplementation.
35055177	8	3	theme	fat-diet	1165:1172	arg1	group					1174:1178	the fat-diet group	1161:1178	the fat-diet group	1161:1178	The family Prevotellaceae was also significantly increased in the fat-diet group.
35055177	7	4	theme	intestinal	873:882	arg1	microbiota					884:893	the intestinal microbiota	869:893	the intestinal microbiota	869:893	We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia&nbsp;muciniphila increased by 5-fold with inulin supplementation.
35055177	1	5	theme	microbiota	254:263	arg1	disturbances					265:276	intestinal microbiota disturbances	243:276	intestinal microbiota disturbances	243:276	Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances.
35055177	9	6	from	remodeling	1277:1286	arg1	composition					1317:1327	the intestinal microbiota composition	1291:1327	the intestinal microbiota composition	1291:1327	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	4	7	theme	rodent	516:521	arg1	diet					523:526	a standard rodent diet	505:526	a standard rodent diet	505:526	Mice exposed to a standard rodent diet or a fat-enriched diet, were supplemented or not, with inulin.
35055177	5	8	theme	mice	701:704	arg1	samples					712:718	mice fecal samples	701:718	mice fecal samples	701:718	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	4	9	theme	standard	507:514	arg1	diet					523:526	a standard rodent diet	505:526	a standard rodent diet	505:526	Mice exposed to a standard rodent diet or a fat-enriched diet, were supplemented or not, with inulin.
35055177	5	10	theme	Liver	591:595	arg1	histology					597:605	Liver histology	591:605	Liver histology	591:605	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	5	11	theme	fecal	706:710	arg1	samples					712:718	mice fecal samples	701:718	mice fecal samples	701:718	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	5	12	theme	H&E	640:642	arg1	staining					644:651	H&E staining	640:651	H&E staining	640:651	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	1	13	from	hepatocytes	168:178	arg1	response					183:190	response	183:190	response to a high calorie intake	183:215	Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances.
35055177	2	14	with	polysaccharide	325:338	arg1	content					366:372	a high dietary fiber content	345:372	a high dietary fiber content	345:372	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	6	15	theme	liver	782:786	arg1	steatosis					788:796	liver steatosis	782:796	liver steatosis	782:796	Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group.
35055177	1	16	theme	calorie	202:208	arg1	intake					210:215	a high calorie intake	195:215	a high calorie intake	195:215	Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances.
35055177	9	17	theme	inulin	1206:1211	arg1	supplementation					1213:1227	inulin supplementation	1206:1227	inulin supplementation in liver steatosis-affected animals	1206:1263	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	2	18	theme	prebiotic	283:291	arg1	polysaccharide					325:338	a naturally occurring polysaccharide	303:338	a naturally occurring polysaccharide with a high dietary fiber content	303:372	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	2	18	theme	prebiotic	283:291	arg1	inulin					293:298	The prebiotic inulin	279:298	The prebiotic inulin	279:298	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	1	19	theme	high	197:200	arg1	intake					210:215	a high calorie intake	195:215	a high calorie intake	195:215	Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances.
35055177	3	20	from	effect	397:402	arg1	microbiota					432:441	the intestinal microbiota	417:441	the intestinal microbiota	417:441	Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model.
35055177	7	21	theme	inulin	1075:1080	arg1	supplementation					1082:1096	inulin supplementation	1075:1096	inulin supplementation	1075:1096	We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia&nbsp;muciniphila increased by 5-fold with inulin supplementation.
35055177	5	22	theme	16S	723:725	arg1	sequencing					732:741	16S rRNA sequencing	723:741	16S rRNA sequencing	723:741	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	1	23	theme	Hepatic	97:103	arg1	steatosis					105:113	Hepatic steatosis	97:113	Hepatic steatosis	97:113	Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances.
35055177	3	24	theme	intestinal	421:430	arg1	microbiota					432:441	the intestinal microbiota	417:441	the intestinal microbiota	417:441	Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model.
35055177	5	25	theme	oil	626:628	arg1	O					634:634	oil red O	626:634	oil red O	626:634	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	9	26	theme	liver	1399:1403	arg1	steatosis					1405:1413	liver steatosis	1399:1413	liver steatosis	1399:1413	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	0	27	theme	Hepatic	29:35	arg1	Steatosis					37:45	Diet-Induced Hepatic Steatosis	16:45	Diet-Induced Hepatic Steatosis	16:45	Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level.
35055177	6	28	theme	Inulin	744:749	arg1	treatment					751:759	Inulin treatment	744:759	Inulin treatment	744:759	Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group.
35055177	2	29	theme	occurring	315:323	arg1	polysaccharide					325:338	a naturally occurring polysaccharide	303:338	a naturally occurring polysaccharide with a high dietary fiber content	303:372	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	2	29	theme	occurring	315:323	arg1	inulin					293:298	The prebiotic inulin	279:298	The prebiotic inulin	279:298	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	3	30	theme	liver	468:472	arg1	disease					474:480	a non-alcoholic fatty liver disease	446:480	a non-alcoholic fatty liver disease model	446:486	Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model.
35055177	0	31	theme	Diet-Induced	16:27	arg1	Steatosis					37:45	Diet-Induced Hepatic Steatosis	16:45	Diet-Induced Hepatic Steatosis	16:45	Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level.
35055177	9	32	theme	steatosis-affected	1238:1255	arg1	animals					1257:1263	liver steatosis-affected animals	1232:1263	liver steatosis-affected animals	1232:1263	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	3	33	theme	disease	474:480	arg1	model					482:486	a non-alcoholic fatty liver disease model	446:486	a non-alcoholic fatty liver disease model	446:486	Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model.
35055177	2	34	theme	dietary	352:358	arg1	content					366:372	a high dietary fiber content	345:372	a high dietary fiber content	345:372	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	3	35	theme	non-alcoholic	448:460	arg1	disease					474:480	a non-alcoholic fatty liver disease	446:480	a non-alcoholic fatty liver disease model	446:486	Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model.
35055177	3	36	theme	fatty	462:466	arg1	disease					474:480	a non-alcoholic fatty liver disease	446:480	a non-alcoholic fatty liver disease model	446:486	Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model.
35055177	0	37	theme	Intestinal	61:70	arg1	Level					90:94	Intestinal Akkermansia Genus Level	61:94	Intestinal Akkermansia Genus Level	61:94	Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level.
35055177	7	38	dep	re-shaped	859:867	arg1	increased					957:965	increased	957:965	were Verrucomicrobia genus significantly increased in the fat-diet group	916:987	We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia&nbsp;muciniphila increased by 5-fold with inulin supplementation.
35055177	5	39	theme	rRNA	727:730	arg1	sequencing					732:741	16S rRNA sequencing	723:741	16S rRNA sequencing	723:741	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	2	40	theme	fiber	360:364	arg1	content					366:372	a high dietary fiber content	345:372	a high dietary fiber content	345:372	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	3	41	theme	inulin	407:412	arg1	effect					397:402	the effect	393:402	the effect of inulin on the intestinal microbiota	393:441	Here, we evaluate the effect of inulin on the intestinal microbiota in a non-alcoholic fatty liver disease model.
35055177	4	42	theme	fat-enriched	533:544	arg1	diet					546:549	a fat-enriched diet	531:549	a fat-enriched diet	531:549	Mice exposed to a standard rodent diet or a fat-enriched diet, were supplemented or not, with inulin.
35055177	8	43	theme	family	1103:1108	arg1	Prevotellaceae					1110:1123	The family Prevotellaceae	1099:1123	The family Prevotellaceae	1099:1123	The family Prevotellaceae was also significantly increased in the fat-diet group.
35055177	1	44	theme	triglyceride	135:146	arg1	accumulation					148:159	triglyceride accumulation	135:159	triglyceride accumulation within hepatocytes in response to a high calorie intake	135:215	Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances.
35055177	9	45	theme	liver	1232:1236	arg1	animals					1257:1263	liver steatosis-affected animals	1232:1263	liver steatosis-affected animals	1232:1263	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	2	46	theme	high	347:350	arg1	content					366:372	a high dietary fiber content	345:372	a high dietary fiber content	345:372	The prebiotic inulin is a naturally occurring polysaccharide with a high dietary fiber content.
35055177	7	47	theme	phylum	902:907	arg1	level					909:913	the phylum level	898:913	the phylum level	898:913	We also observed that inulin re-shaped the intestinal microbiota at the phylum level, were Verrucomicrobia genus significantly increased in the fat-diet group; specifically, we observed that Akkermansia&nbsp;muciniphila increased by 5-fold with inulin supplementation.
35055177	5	48	theme	intestinal	661:670	arg1	microbiota					672:681	the intestinal microbiota	657:681	the intestinal microbiota	657:681	Liver histology was evaluated with oil red O and H&E staining and the intestinal microbiota was determined in mice fecal samples by 16S rRNA sequencing.
35055177	6	49	theme	diet	818:821	arg1	group					823:827	the fat-enriched diet group	801:827	the fat-enriched diet group	801:827	Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group.
35055177	9	50	from	supplementation	1213:1227	arg1	animals					1257:1263	liver steatosis-affected animals	1232:1263	liver steatosis-affected animals	1232:1263	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	0	51	theme	Genus	84:88	arg1	Level					90:94	Intestinal Akkermansia Genus Level	61:94	Intestinal Akkermansia Genus Level	61:94	Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level.
35055177	6	52	theme	fat-enriched	805:816	arg1	group					823:827	the fat-enriched diet group	801:827	the fat-enriched diet group	801:827	Inulin treatment effectively prevents liver steatosis in the fat-enriched diet group.
35055177	0	53	theme	Akkermansia	72:82	arg1	Level					90:94	Intestinal Akkermansia Genus Level	61:94	Intestinal Akkermansia Genus Level	61:94	Inulin Improves Diet-Induced Hepatic Steatosis and Increases Intestinal Akkermansia Genus Level.
35055177	9	54	theme	intestinal	1295:1304	arg1	composition					1317:1327	the intestinal microbiota composition	1291:1327	the intestinal microbiota composition	1291:1327	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	9	55	theme	lipid	1351:1355	arg1	metabolism					1357:1366	lipid metabolism	1351:1366	lipid metabolism	1351:1366	Overall, we propose that inulin supplementation in liver steatosis-affected animals, promotes a remodeling in the intestinal microbiota composition, which might regulate lipid metabolism, thus contributing to tackling liver steatosis.
35055177	1	56	theme	intestinal	243:252	arg1	disturbances					265:276	intestinal microbiota disturbances	243:276	intestinal microbiota disturbances	243:276	Hepatic steatosis is characterized by triglyceride accumulation within hepatocytes in response to a high calorie intake, and it may be related to intestinal microbiota disturbances.
35098381	3	0	theme	avilamycin	677:686	arg1	yields					667:672	yields	667:672	yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT)	667:775	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	5	1	theme	oxygen	1052:1057	arg1	vectors					1059:1065	oxygen vectors	1052:1065	oxygen vectors	1052:1065	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	1	2	theme	Streptomyces	183:194	arg1	viridochromogenes					196:212	Streptomyces viridochromogenes	183:212	Streptomyces viridochromogenes	183:212	Avilamycin, an excellent growth-promoting feed additive, produced by Streptomyces viridochromogenes, was widely used to promote the growth of poultry by inhibiting Gram-positive bacteria.
35098381	1	3	theme	Gram-positive	278:290	arg1	bacteria					292:299	Gram-positive bacteria	278:299	Gram-positive bacteria	278:299	Avilamycin, an excellent growth-promoting feed additive, produced by Streptomyces viridochromogenes, was widely used to promote the growth of poultry by inhibiting Gram-positive bacteria.
35098381	4	4	theme	growth	904:909	arg1	abilities					863:871	stronger abilities	854:871	stronger abilities of substrate consumption, cell growth and antibiotic synthesis	854:934	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	5	5	theme	glucose	1068:1074	arg1	medium					990:995	fermentation medium	977:995	fermentation medium	977:995	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	5	theme	glucose	1068:1074	arg1	strategies					1038:1047	supplementation strategies	1022:1047	supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate)	1022:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	6	theme	mutant	1182:1187	arg1	F-23					1196:1199	the mutant strain F-23	1178:1199	the mutant strain F-23	1178:1199	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	3	7	dep	screened	648:655	arg1	out					657:659	out	657:659	out	657:659	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	4	8	theme	Z-6	817:819	arg1	strains					842:848	Z-6, A-9 and F-23 mutant strains	817:848	Z-6, A-9 and F-23 mutant strains	817:848	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	3	9	theme	high-yielding	554:566	arg1	strains					575:581	The avilamycin high-yielding mutant strains	539:581	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L)	539:641	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	5	10	theme	strain	1189:1194	arg1	F-23					1196:1199	the mutant strain F-23	1178:1199	the mutant strain F-23	1178:1199	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	3	11	theme	wild	760:763	arg1	strain					765:770	the wild strain	756:770	the wild strain (WT)	756:775	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	3	11	theme	wild	760:763	arg1	WT					773:774	WT	773:774	WT	773:774	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	2	12	theme	avilamycin	527:536	arg1	production					513:522	the production	509:522	the production of avilamycin	509:536	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	5	13	theme	supplementation	1022:1036	arg1	medium					990:995	fermentation medium	977:995	fermentation medium	977:995	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	13	theme	supplementation	1022:1036	arg1	strategies					1038:1047	supplementation strategies	1022:1047	supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate)	1022:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	3	14	theme	mutant	568:573	arg1	strains					575:581	The avilamycin high-yielding mutant strains	539:581	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L)	539:641	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	5	15	theme	fermentation	1241:1252	arg1	optimization					1254:1265	fermentation optimization	1241:1265	fermentation optimization	1241:1265	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	4	16	theme	consumption	886:896	arg1	abilities					863:871	stronger abilities	854:871	stronger abilities of substrate consumption, cell growth and antibiotic synthesis	854:934	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	0	17	from	fermentation	53:64	arg1	viridochromogenes					95:111	Streptomyces viridochromogenes	82:111	Streptomyces viridochromogenes	82:111	Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.
35098381	6	18	from	synthesis	1424:1432	arg1	Streptomyces					1468:1479	other Streptomyces	1462:1479	other Streptomyces	1462:1479	In summary, the strategy of increasing the production of avilamycin in S. viridochromogenes in this work might provide an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces.
35098381	4	19	theme	substrate	876:884	arg1	consumption					886:896	substrate consumption	876:896	substrate consumption	876:896	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	5	20	dep	precursors	1080:1089	arg1	L-valine					1092:1099	L-valine	1092:1099	L-valine	1092:1099	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	20	dep	precursors	1080:1089	arg1	precursors					1080:1089	precursors	1080:1089	precursors (L-valine, D-xylose and sodium acetate)	1080:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	20	dep	precursors	1080:1089	arg1	D-xylose					1102:1109	D-xylose	1102:1109	D-xylose	1102:1109	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	20	dep	precursors	1080:1089	arg1	acetate					1122:1128	sodium acetate	1115:1128	sodium acetate	1115:1128	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	2	21	dep	ARTP	381:384	arg1	Atmospheric					387:397	Atmospheric	387:397	Atmospheric	387:397	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	2	21	dep	ARTP	381:384	arg1	temperature					408:418	room temperature	403:418	room temperature	403:418	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	6	22	theme	other	1462:1466	arg1	Streptomyces					1468:1479	other Streptomyces	1462:1479	other Streptomyces	1462:1479	In summary, the strategy of increasing the production of avilamycin in S. viridochromogenes in this work might provide an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces.
35098381	2	23	theme	room	403:406	arg1	temperature					408:418	room temperature	403:418	room temperature	403:418	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	2	24	theme	high	455:458	arg1	concentrations					460:473	high concentrations	455:473	high concentrations of CaCl2	455:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	0	25	from	mutagenesis	37:47	arg1	viridochromogenes					95:111	Streptomyces viridochromogenes	82:111	Streptomyces viridochromogenes	82:111	Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.
35098381	4	26	theme	performance	782:792	arg1	comparison					794:803	The performance comparison	778:803	The performance comparison	778:803	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	4	27	theme	cell	899:902	arg1	growth					904:909	cell growth	899:909	cell growth	899:909	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	2	28	theme	mutagenesis	345:355	arg1	methods					320:326	the methods	316:326	the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2	316:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	0	29	theme	Avilamycin	0:9	arg1	production					11:20	Avilamycin production	0:20	Avilamycin production	0:20	Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.
35098381	2	30	theme	combinational	331:343	arg1	mutagenesis					345:355	combinational mutagenesis	331:355	combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma)	331:426	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	4	31	contain	had	850:852	arg1	strains					842:848	Z-6, A-9 and F-23 mutant strains	817:848	Z-6, A-9 and F-23 mutant strains	817:848	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	4	31	contain	had	850:852	arg2	abilities					863:871	stronger abilities	854:871	stronger abilities of substrate consumption, cell growth and antibiotic synthesis	854:934	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	2	32	theme	screening	442:450	arg1	methods					320:326	the methods	316:326	the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2	316:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	6	33	from	production	1311:1320	arg1	viridochromogenes					1342:1358	S. viridochromogenes	1339:1358	S. viridochromogenes	1339:1358	In summary, the strategy of increasing the production of avilamycin in S. viridochromogenes in this work might provide an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces.
35098381	6	34	theme	secondary	1437:1445	arg1	metabolites					1447:1457	secondary metabolites	1437:1457	secondary metabolites	1437:1457	In summary, the strategy of increasing the production of avilamycin in S. viridochromogenes in this work might provide an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces.
35098381	2	35	theme	rational	433:440	arg1	screening					442:450	rational screening	433:450	rational screening by high concentrations of CaCl2	433:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	5	36	theme	sodium	1115:1120	arg1	precursors					1080:1089	precursors	1080:1089	precursors (L-valine, D-xylose and sodium acetate)	1080:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	36	theme	sodium	1115:1120	arg1	acetate					1122:1128	sodium acetate	1115:1128	sodium acetate	1115:1128	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	4	37	theme	mutant	835:840	arg1	strains					842:848	Z-6, A-9 and F-23 mutant strains	817:848	Z-6, A-9 and F-23 mutant strains	817:848	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	6	38	theme	metabolites	1447:1457	arg1	synthesis					1424:1432	the synthesis	1420:1432	the synthesis of secondary metabolites in other Streptomyces	1420:1479	In summary, the strategy of increasing the production of avilamycin in S. viridochromogenes in this work might provide an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces.
35098381	4	39	theme	synthesis	926:934	arg1	abilities					863:871	stronger abilities	854:871	stronger abilities of substrate consumption, cell growth and antibiotic synthesis	854:934	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	4	40	theme	stronger	854:861	arg1	abilities					863:871	stronger abilities	854:871	stronger abilities of substrate consumption, cell growth and antibiotic synthesis	854:934	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	5	41	theme	inoculation	998:1008	arg1	medium					990:995	fermentation medium	977:995	fermentation medium	977:995	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	41	theme	inoculation	998:1008	arg1	parameters					1010:1019	inoculation parameters	998:1019	inoculation parameters	998:1019	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	2	42	theme	CaCl2	478:482	arg1	concentrations					460:473	high concentrations	455:473	high concentrations of CaCl2	455:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	5	43	theme	precursors	1080:1089	arg1	medium					990:995	fermentation medium	977:995	fermentation medium	977:995	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	43	theme	precursors	1080:1089	arg1	strategies					1038:1047	supplementation strategies	1022:1047	supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate)	1022:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	4	44	theme	antibiotic	915:924	arg1	synthesis					926:934	antibiotic synthesis	915:934	antibiotic synthesis	915:934	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	3	45	theme	F-23	625:628	arg1	strains					575:581	The avilamycin high-yielding mutant strains	539:581	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L)	539:641	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	3	46	theme	A-9	604:606	arg1	strains					575:581	The avilamycin high-yielding mutant strains	539:581	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L)	539:641	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	6	47	theme	avilamycin	1325:1334	arg1	production					1311:1320	the production	1307:1320	the production of avilamycin in S. viridochromogenes	1307:1358	In summary, the strategy of increasing the production of avilamycin in S. viridochromogenes in this work might provide an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces.
35098381	4	48	theme	A-9	822:824	arg1	strains					842:848	Z-6, A-9 and F-23 mutant strains	817:848	Z-6, A-9 and F-23 mutant strains	817:848	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	2	49	used	utilized	489:496	arg2	methods					320:326	the methods	316:326	the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2	316:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	0	50	dep	mutagenesis	37:47	arg1	optimization					66:77	optimization	66:77	optimization	66:77	Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.
35098381	5	51	theme	F-23	1196:1199	arg1	yield					1169:1173	the avilamycin yield	1154:1173	the avilamycin yield of the mutant strain F-23	1154:1199	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	3	52	theme	Z-6	586:588	arg1	strains					575:581	The avilamycin high-yielding mutant strains	539:581	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L)	539:641	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	4	53	theme	F-23	830:833	arg1	strains					842:848	Z-6, A-9 and F-23 mutant strains	817:848	Z-6, A-9 and F-23 mutant strains	817:848	The performance comparison showed that Z-6, A-9 and F-23 mutant strains had stronger abilities of substrate consumption, cell growth and antibiotic synthesis than WT.
35098381	1	54	used	used	226:229	arg2	Avilamycin					114:123	Avilamycin	114:123	Avilamycin	114:123	Avilamycin, an excellent growth-promoting feed additive, produced by Streptomyces viridochromogenes, was widely used to promote the growth of poultry by inhibiting Gram-positive bacteria.
35098381	1	54	used	used	226:229	arg2	additive					161:168	additive	161:168	additive	161:168	Avilamycin, an excellent growth-promoting feed additive, produced by Streptomyces viridochromogenes, was widely used to promote the growth of poultry by inhibiting Gram-positive bacteria.
35098381	2	55	theme	UV	360:361	arg1	screening					442:450	rational screening	433:450	rational screening by high concentrations of CaCl2	433:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	2	55	theme	UV	360:361	arg1	mutagenesis					345:355	combinational mutagenesis	331:355	combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma)	331:426	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	3	56	theme	avilamycin	543:552	arg1	strains					575:581	The avilamycin high-yielding mutant strains	539:581	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L)	539:641	The avilamycin high-yielding mutant strains of Z-6 (29.31 mg/L), A-9 (36.84 mg/L) and F-23 (45.73 mg/L) were screened out, with yields of avilamycin improved by 57.92%, 98.49% and 146.39%, respectively, compared with the wild strain (WT).
35098381	5	57	theme	fermentation	977:988	arg1	medium					990:995	fermentation medium	977:995	fermentation medium	977:995	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	57	theme	fermentation	977:988	arg1	parameters					1010:1019	inoculation parameters	998:1019	inoculation parameters	998:1019	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	57	theme	fermentation	977:988	arg1	strategies					1038:1047	supplementation strategies	1022:1047	supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate)	1022:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	2	58	dep	Atmospheric	387:397	arg1	plasma					420:425	plasma	420:425	plasma	420:425	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	1	59	theme	poultry	256:262	arg1	growth					246:251	the growth	242:251	the growth of poultry	242:262	Avilamycin, an excellent growth-promoting feed additive, produced by Streptomyces viridochromogenes, was widely used to promote the growth of poultry by inhibiting Gram-positive bacteria.
35098381	0	60	theme	Streptomyces	82:93	arg1	viridochromogenes					95:111	Streptomyces viridochromogenes	82:111	Streptomyces viridochromogenes	82:111	Avilamycin production enhancement by mutagenesis and fermentation optimization in Streptomyces viridochromogenes.
35098381	5	61	theme	medium	990:995	arg1	composition					962:972	the composition	958:972	the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate)	958:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	62	theme	avilamycin	1158:1167	arg1	yield					1169:1173	the avilamycin yield	1154:1173	the avilamycin yield of the mutant strain F-23	1154:1199	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	63	theme	vectors	1059:1065	arg1	medium					990:995	fermentation medium	977:995	fermentation medium	977:995	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	5	63	theme	vectors	1059:1065	arg1	strategies					1038:1047	supplementation strategies	1022:1047	supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate)	1022:1129	Furthermore, the composition of fermentation medium, inoculation parameters, supplementation strategies of oxygen vectors, glucose and precursors (L-valine, D-xylose and sodium acetate) had been optimized and the avilamycin yield of the mutant strain F-23 was significantly enhanced by 41.87% by fermentation optimization.
35098381	2	64	theme	ARTP	381:384	arg1	screening					442:450	rational screening	433:450	rational screening by high concentrations of CaCl2	433:482	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	2	64	theme	ARTP	381:384	arg1	mutagenesis					345:355	combinational mutagenesis	331:355	combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma)	331:426	In this work, the methods of combinational mutagenesis of UV (Ultraviolet) and ARTP (Atmospheric and room temperature plasma), and rational screening by high concentrations of CaCl2 were utilized to promote the production of avilamycin.
35098381	6	65	theme	alternative	1390:1400	arg1	method					1402:1407	an alternative method	1387:1407	an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces	1387:1479	In summary, the strategy of increasing the production of avilamycin in S. viridochromogenes in this work might provide an alternative method to enhance the synthesis of secondary metabolites in other Streptomyces.
36323316	0	0	theme	children	69:76	arg1	gut					50:52	the gut	46:52	the gut of Bangladeshi children	46:76	A distinct clade of Bifidobacterium longum in the gut of Bangladeshi children thrives during weaning.
36323316	0	1	from	clade	11:15	arg1	gut					50:52	the gut	46:52	the gut of Bangladeshi children	46:76	A distinct clade of Bifidobacterium longum in the gut of Bangladeshi children thrives during weaning.
36323316	5	2	theme	associated	702:711	arg1	metabolites					713:723	associated metabolites	702:723	associated metabolites	702:723	We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp.
36323316	4	3	theme	gut	645:647	arg1	ecology					649:655	gut ecology	645:655	gut ecology	645:655	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	5	4	theme	B. longum	681:689	arg1	clades					691:696	the B. longum clades	677:696	the B. longum clades	677:696	We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp.
36323316	0	5	theme	Bangladeshi	57:67	arg1	children					69:76	Bangladeshi children	57:76	Bangladeshi children	57:76	A distinct clade of Bifidobacterium longum in the gut of Bangladeshi children thrives during weaning.
36323316	7	6	theme	cultural	938:945	arg1	heterogeneity					973:985	geographic, cultural, seasonal, and ecological heterogeneity	926:985	geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development	926:1110	Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.
36323316	7	7	dep	development	1100:1110	arg1	benefiting					1082:1091	benefiting	1082:1091	benefiting	1082:1091	Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.
36323316	4	8	theme	lower	537:541	arg1	prevalence					543:552	lower prevalence	537:552	lower prevalence	537:552	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	4	9	dep	prevalent	491:499	arg1	prevalence					543:552	lower prevalence	537:552	lower prevalence	537:552	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	3	10	theme	longum	336:341	arg1	clade					343:347	A distinct Bifidobacterium longum clade	309:347	A distinct Bifidobacterium longum clade	309:347	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	7	11	theme	geographic	926:935	arg1	heterogeneity					973:985	geographic, cultural, seasonal, and ecological heterogeneity	926:985	geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development	926:1110	Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.
36323316	2	12	from	microbiome	204:213	arg1	Bangladesh					262:271	Bangladesh	262:271	Bangladesh	262:271	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	5	13	theme	childhood	744:752	arg1	diarrhea					754:761	childhood diarrhea	744:761	childhood diarrhea	744:761	We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp.
36323316	3	14	dep	substrates	458:467	arg1	solid					447:451	solid	447:451	solid	447:451	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	3	14	dep	substrates	458:467	arg1	both					426:429	both	426:429	both	426:429	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	1	15	theme	infant	146:151	arg1	health					153:158	infant health	146:158	infant health	146:158	The gut microbiome has an important role in infant health and development.
36323316	3	16	theme	distinct	311:318	arg1	clade					343:347	A distinct Bifidobacterium longum clade	309:347	A distinct Bifidobacterium longum clade	309:347	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	3	17	theme	foods	385:389	arg1	introduction					363:374	introduction	363:374	introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates	363:467	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	2	18	from	metabolome	219:228	arg1	Bangladesh					262:271	Bangladesh	262:271	Bangladesh	262:271	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	5	19	theme	growth	821:826	arg1	measures					828:835	growth measures	821:835	growth measures	821:835	We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp.
36323316	0	20	theme	distinct	2:9	arg1	clade					11:15	A distinct clade	0:15	A distinct clade of Bifidobacterium longum in the gut of Bangladeshi children	0:76	A distinct clade of Bifidobacterium longum in the gut of Bangladeshi children thrives during weaning.
36323316	3	21	theme	harbored	395:402	arg1	enzymes					404:410	harbored enzymes	395:410	harbored enzymes	395:410	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	2	22	theme	first	284:288	arg1	years					294:298	the first two years	280:298	the first two years of life	280:306	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	3	23	theme	enzymes	404:410	arg1	introduction					363:374	introduction	363:374	introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates	363:467	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	5	24	theme	early	767:771	arg1	growth					773:778	early growth	767:778	early growth	767:778	We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp.
36323316	4	25	theme	present	516:522	arg1	Bangladesh					504:513	Bangladesh	504:513	Bangladesh	504:513	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	7	26	theme	infant	1093:1098	arg1	development					1100:1110	and potentially benefiting infant development	1066:1110	development	1100:1110	Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.
36323316	2	27	dep	microbiome	204:213	arg1	the					194:196	the	194:196	the	194:196	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	7	28	theme	ecological	962:971	arg1	heterogeneity					973:985	geographic, cultural, seasonal, and ecological heterogeneity	926:985	geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development	926:1110	Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.
36323316	4	29	theme	other	581:585	arg1	taxa					591:594	many other gut taxa	576:594	many other gut taxa	576:594	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	0	30	theme	longum	36:41	arg1	clade					11:15	A distinct clade	0:15	A distinct clade of Bifidobacterium longum in the gut of Bangladeshi children	0:76	A distinct clade of Bifidobacterium longum in the gut of Bangladeshi children thrives during weaning.
36323316	1	31	theme	gut	106:108	arg1	microbiome					110:119	The gut microbiome	102:119	The gut microbiome	102:119	The gut microbiome has an important role in infant health and development.
36323316	7	32	theme	microbiome	1033:1042	arg1	factors					1044:1050	microbiome factors	1033:1050	microbiome factors implicated in and potentially benefiting infant development	1033:1110	Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.
36323316	5	33	theme	positive	791:798	arg1	associations					800:811	positive associations	791:811	positive associations between growth measures and B. longum subsp	791:855	We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp.
36323316	4	34	from	Bangladesh	504:513	arg1	clade					474:478	The clade	470:478	The clade	470:478	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	4	34	from	Bangladesh	504:513	arg1	prevalent					491:499	prevalent	491:499	prevalent	491:499	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	3	35	theme	breast	431:436	arg1	milk					438:441	breast milk	431:441	breast milk	431:441	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	4	36	from	prevalent	491:499	arg1	Bangladesh					504:513	Bangladesh	504:513	Bangladesh	504:513	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	3	37	theme	solid	379:383	arg1	foods					385:389	solid foods	379:389	solid foods	379:389	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	2	38	theme	fecal	198:202	arg1	microbiome					204:213	fecal microbiome	198:213	fecal microbiome	198:213	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	2	39	theme	life	303:306	arg1	years					294:298	the first two years	280:298	the first two years of life	280:306	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	3	40	dep	both	426:429	arg1	milk					438:441	breast milk	431:441	breast milk	431:441	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	2	41	theme	children	243:250	arg1	metabolome					219:228	metabolome	219:228	metabolome	219:228	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	2	41	theme	children	243:250	arg1	microbiome					204:213	fecal microbiome	198:213	fecal microbiome	198:213	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	4	42	theme	important	627:635	arg1	role					637:640	an important role	624:640	an important role	624:640	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	2	43	theme	young	237:241	arg1	children					243:250	222 young children	233:250	222 young children	233:250	We characterized the fecal microbiome and metabolome of 222 young children in Dhaka, Bangladesh during the first two years of life.
36323316	3	44	theme	food	453:456	arg1	substrates					458:467	both breast milk and solid food substrates	426:467	both breast milk and solid food substrates	426:467	A distinct Bifidobacterium longum clade expanded with introduction of solid foods and harbored enzymes for utilizing both breast milk and solid food substrates.
36323316	4	45	theme	gut	587:589	arg1	taxa					591:594	many other gut taxa	576:594	many other gut taxa	576:594	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	5	46	theme	B. longum	841:849	arg1	subsp					851:855	B. longum subsp	841:855	B. longum subsp	841:855	We also found that the B. longum clades and associated metabolites were implicated in childhood diarrhea and early growth, including positive associations between growth measures and B. longum subsp.
36323316	4	47	theme	many	576:579	arg1	taxa					591:594	many other gut taxa	576:594	many other gut taxa	576:594	The clade was highly prevalent in Bangladesh, present globally (at lower prevalence), and correlated with many other gut taxa and metabolites, indicating an important role in gut ecology.
36323316	1	48	contain	has	121:123	arg1	microbiome					110:119	The gut microbiome	102:119	The gut microbiome	102:119	The gut microbiome has an important role in infant health and development.
36323316	1	48	contain	has	121:123	arg2	role					138:141	an important role	125:141	an important role	125:141	The gut microbiome has an important role in infant health and development.
36323316	7	49	theme	seasonal	948:955	arg1	heterogeneity					973:985	geographic, cultural, seasonal, and ecological heterogeneity	926:985	geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development	926:1110	Our data demonstrate geographic, cultural, seasonal, and ecological heterogeneity that should be accounted for when identifying microbiome factors implicated in and potentially benefiting infant development.
36323316	1	50	theme	important	128:136	arg1	role					138:141	an important role	125:141	an important role	125:141	The gut microbiome has an important role in infant health and development.
36856084	7	0	theme	thermophilic	1084:1095	arg1	bacterium					1097:1105	a thermophilic bacterium	1082:1105	a thermophilic bacterium	1082:1105	Biologically pretreated SB was fermented by a thermophilic bacterium, Neobacillus sedimentimangrovi UE25, that yielded 8.64 IU mL-1 of cellulase.
36856084	1	1	theme	promising	135:143	arg1	bagasse					117:123	Sugarcane bagasse	107:123	Sugarcane bagasse (SB)	107:128	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	1	1	theme	promising	135:143	arg1	source					145:150	a promising source	133:150	a promising source of appreciable quantities of fermentable sugars	133:198	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	9	2	theme	corrosive	1397:1405	arg1	chemicals					1407:1415	corrosive chemicals	1397:1415	corrosive chemicals	1397:1415	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	0	3	theme	laccase	74:80	arg1	production					53:62	the production	49:62	the production of fungal laccase and bacterial cellulase	49:104	Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase.
36856084	9	4	theme	synchronized	1289:1300	arg1	process					1302:1308	The synchronized process	1285:1308	The synchronized process	1285:1308	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	6	5	from	improvement	983:993	arg1	titers					1002:1007	the titers	998:1007	the titers	998:1007	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	6	6	theme	incubation	798:807	arg1	period					809:814	incubation period	798:814	incubation period	798:814	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	9	7	theme	chemicals	1407:1415	arg1	use					1390:1392	the minimum use	1378:1392	the minimum use of corrosive chemicals	1378:1415	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	0	8	theme	cellulase	96:104	arg1	production					53:62	the production	49:62	the production of fungal laccase and bacterial cellulase	49:104	Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase.
36856084	6	9	theme	particle	877:884	arg1	size					886:889	particle size	877:889	particle size	877:889	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	3	10	with	synchronized	387:398	arg1	production					409:418	the production	405:418	the production of a thermostable cellulase using SB as a raw material	405:473	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	4	11	theme	solid	571:575	arg1	fermentation					583:594	solid state fermentation	571:594	solid state fermentation (SSF)	571:600	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	4	11	theme	solid	571:575	arg1	SSF					597:599	SSF	597:599	SSF	597:599	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	0	12	theme	bacterial	86:94	arg1	cellulase					96:104	bacterial cellulase	86:104	bacterial cellulase	86:104	Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase.
36856084	9	13	theme	laccase	1335:1341	arg1	titers					1325:1330	higher titers	1318:1330	higher titers of laccase and cellulase	1318:1355	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	9	14	theme	higher	1318:1323	arg1	titers					1325:1330	higher titers	1318:1330	higher titers of laccase and cellulase	1318:1355	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	2	15	theme	carbohydrates	262:274	arg1	utilization					241:251	utilization	241:251	utilization of these carbohydrates	241:274	However, the presence of lignin hinders utilization of these carbohydrates and hence pretreatment to remove lignin is necessarily carried out.
36856084	4	16	theme	laccase	633:639	arg1	titer					608:612	a titer	606:612	a titer of 1758 IU mL-1 of laccase	606:639	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	3	17	theme	thermostable	425:436	arg1	cellulase					438:446	a thermostable cellulase	423:446	a thermostable cellulase using SB as a raw material	423:473	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	5	18	theme	Plackett	723:730	arg1	design					739:744	Plackett Burman design	723:744	Plackett Burman design	723:744	Investigations of nine factors affecting laccase production through Plackett Burman design improved the titers to 6539 IU mL-1 .
36856084	9	19	theme	minimum	1382:1388	arg1	use					1390:1392	the minimum use	1378:1392	the minimum use of corrosive chemicals	1378:1415	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	1	20	theme	appreciable	155:165	arg1	quantities					167:176	appreciable quantities	155:176	appreciable quantities of fermentable sugars	155:198	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	6	21	theme	moisture	855:862	arg1	content					864:870	moisture content	855:870	moisture content	855:870	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	7	22	theme	pretreated	1051:1060	arg1	SB					1062:1063	Biologically pretreated SB	1038:1063	Biologically pretreated SB	1038:1063	Biologically pretreated SB was fermented by a thermophilic bacterium, Neobacillus sedimentimangrovi UE25, that yielded 8.64 IU mL-1 of cellulase.
36856084	5	23	theme	Burman	732:737	arg1	design					739:744	Plackett Burman design	723:744	Plackett Burman design	723:744	Investigations of nine factors affecting laccase production through Plackett Burman design improved the titers to 6539 IU mL-1 .
36856084	1	24	theme	quantities	167:176	arg1	bagasse					117:123	Sugarcane bagasse	107:123	Sugarcane bagasse (SB)	107:128	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	1	24	theme	quantities	167:176	arg1	source					145:150	a promising source	133:150	a promising source of appreciable quantities of fermentable sugars	133:198	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	0	25	theme	Biological	0:9	arg1	pretreatment					11:22	Biological pretreatment	0:22	Biological pretreatment of sugarcane	0:35	Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase.
36856084	6	26	theme	Composite	952:960	arg1	design					962:967	Central Composite design	944:967	Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 )	944:1035	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	7	27	dep	sedimentimangrovi	1120:1136	arg1	Neobacillus					1108:1118	Neobacillus	1108:1118	Neobacillus	1108:1118	Biologically pretreated SB was fermented by a thermophilic bacterium, Neobacillus sedimentimangrovi UE25, that yielded 8.64 IU mL-1 of cellulase.
36856084	9	28	theme	SB	1370:1371	arg1	SSF					1363:1365	SSF	1363:1365	SSF of SB	1363:1371	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	6	29	dep	factors	789:795	arg1	period					809:814	incubation period	798:814	incubation period	798:814	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	8	30	theme	structural	1243:1252	arg1	analysis					1254:1261	structural analysis	1243:1261	structural analysis	1243:1261	Delignification and cellulose utilization were affirmed by structural analysis through FTIR and SEM.
36856084	3	31	theme	biological	352:361	arg1	method					376:381	a biological pretreatment method	350:381	a biological pretreatment method	350:381	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	3	31	theme	biological	352:361	arg1	synchronized					387:398	synchronized	387:398	synchronized	387:398	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	6	32	theme	Central	944:950	arg1	design					962:967	Central Composite design	944:967	Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 )	944:1035	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	1	33	theme	fermentable	181:191	arg1	sugars					193:198	fermentable sugars	181:198	fermentable sugars	181:198	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	3	34	theme	pretreatment	363:374	arg1	method					376:381	a biological pretreatment method	350:381	a biological pretreatment method	350:381	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	3	34	theme	pretreatment	363:374	arg1	synchronized					387:398	synchronized	387:398	synchronized	387:398	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	9	35	theme	cellulase	1347:1355	arg1	titers					1325:1330	higher titers	1318:1330	higher titers of laccase and cellulase	1318:1355	The synchronized process yielded higher titers of laccase and cellulase under SSF of SB with the minimum use of corrosive chemicals.
36856084	1	36	theme	Sugarcane	107:115	arg1	SB					126:127	SB	126:127	SB	126:127	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	1	36	theme	Sugarcane	107:115	arg1	bagasse					117:123	Sugarcane bagasse	107:123	Sugarcane bagasse (SB)	107:128	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	1	36	theme	Sugarcane	107:115	arg1	source					145:150	a promising source	133:150	a promising source of appreciable quantities of fermentable sugars	133:198	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	1	37	theme	sugars	193:198	arg1	quantities					167:176	appreciable quantities	155:176	appreciable quantities of fermentable sugars	155:198	Sugarcane bagasse (SB) is a promising source of appreciable quantities of fermentable sugars.
36856084	8	38	theme	cellulose	1204:1212	arg1	utilization					1214:1224	cellulose utilization	1204:1224	cellulose utilization	1204:1224	Delignification and cellulose utilization were affirmed by structural analysis through FTIR and SEM.
36856084	4	39	theme	Trametes	540:547	arg1	pubescens					549:557	Trametes pubescens	540:557	Trametes pubescens	540:557	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	5	40	theme	factors	678:684	arg1	Investigations					655:668	Investigations	655:668	Investigations of nine factors affecting laccase production through Plackett Burman design	655:744	Investigations of nine factors affecting laccase production through Plackett Burman design improved the titers to 6539 IU mL-1 .
36856084	4	41	theme	1758 IU mL-1	617:628	arg1	titer					608:612	a titer	606:612	a titer of 1758 IU mL-1 of laccase	606:639	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	4	42	dep	fermented	499:507	arg1	MB					559:560	MB	559:560	MB 89 under solid state fermentation (SSF)	559:600	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	0	43	theme	sugarcane	27:35	arg1	pretreatment					11:22	Biological pretreatment	0:22	Biological pretreatment of sugarcane	0:35	Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase.
36856084	7	44	theme	cellulase	1173:1181	arg1	8.64 IU mL-1					1157:1168	8.64 IU mL-1	1157:1168	8.64 IU mL-1 of cellulase	1157:1181	Biologically pretreated SB was fermented by a thermophilic bacterium, Neobacillus sedimentimangrovi UE25, that yielded 8.64 IU mL-1 of cellulase.
36856084	5	45	theme	laccase	696:702	arg1	production					704:713	laccase production	696:713	laccase production	696:713	Investigations of nine factors affecting laccase production through Plackett Burman design improved the titers to 6539 IU mL-1 .
36856084	6	46	theme	CuSO4	834:838	arg1	concentration					817:829	concentration	817:829	concentration of CuSO4	817:838	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	6	46	theme	CuSO4	834:838	arg1	size					886:889	particle size	877:889	particle size	877:889	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	6	46	theme	CuSO4	834:838	arg1	temperature					842:852	temperature	842:852	temperature	842:852	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	6	46	theme	CuSO4	834:838	arg1	content					864:870	moisture content	855:870	moisture content	855:870	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	4	47	theme	producing	522:530	arg1	fungus					532:537	a laccase producing fungus	512:537	a laccase producing fungus	512:537	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	4	48	theme	laccase	514:520	arg1	fungus					532:537	a laccase producing fungus	512:537	a laccase producing fungus	512:537	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	3	49	theme	raw	462:464	arg1	SB					454:455	SB	454:455	SB	454:455	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	3	49	theme	raw	462:464	arg1	material					466:473	a raw material	460:473	a raw material	460:473	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	6	50	dep	~5	1012:1013	arg1	folds					1015:1019	folds	1015:1019	folds	1015:1019	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	4	51	theme	state	577:581	arg1	fermentation					583:594	solid state fermentation	571:594	solid state fermentation (SSF)	571:600	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	4	51	theme	state	577:581	arg1	SSF					597:599	SSF	597:599	SSF	597:599	Initially, bagasse was fermented by a laccase producing fungus, Trametes pubescens MB 89 under solid state fermentation (SSF) and a titer of 1758 IU mL-1 of laccase was obtained.
36856084	0	52	theme	fungal	67:72	arg1	laccase					74:80	fungal laccase	67:80	fungal laccase	67:80	Biological pretreatment of sugarcane bagasse for the production of fungal laccase and bacterial cellulase.
36856084	3	53	theme	cellulase	438:446	arg1	production					409:418	the production	405:418	the production of a thermostable cellulase using SB as a raw material	405:473	Here, a biological pretreatment method was synchronized with the production of a thermostable cellulase using SB as a raw material.
36856084	6	54	dep	period	809:814	arg1	concentration					817:829	concentration	817:829	concentration of CuSO4	817:838	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	6	54	dep	period	809:814	arg1	size					886:889	particle size	877:889	particle size	877:889	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	6	54	dep	period	809:814	arg1	temperature					842:852	temperature	842:852	temperature	842:852	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	6	54	dep	period	809:814	arg1	content					864:870	moisture content	855:870	moisture content	855:870	Five factors (incubation period, concentration of CuSO4 , temperature, moisture content, and particle size) were found significant which were optimized through Central Composite design leading to an improvement in the titers by ~5 folds (8841 IU mL-1 ).
36856084	2	55	theme	lignin	226:231	arg1	presence					214:221	the presence	210:221	the presence of lignin	210:231	However, the presence of lignin hinders utilization of these carbohydrates and hence pretreatment to remove lignin is necessarily carried out.
36219451	7	0	with	reference	963:971	arg1	system					1037:1042	the xanthine/xanthine oxidase system	1007:1042	the xanthine/xanthine oxidase system	1007:1042	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	0	with	reference	963:971	arg1	Trolox					1048:1053	Trolox	1048:1053	Trolox	1048:1053	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	4	1	theme	composition	402:412	arg1	leaf					475:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf	379:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf	379:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	10	2	theme	IC50	1638:1641	arg1	values					1643:1648	IC50 values	1638:1648	IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox	1638:1732	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	7	3	with	activity	993:1000	arg1	system					1037:1042	the xanthine/xanthine oxidase system	1007:1042	the xanthine/xanthine oxidase system	1007:1042	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	3	with	activity	993:1000	arg1	Trolox					1048:1053	Trolox	1048:1053	Trolox	1048:1053	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	8	4	theme	Several	1089:1095	arg1	volatiles					1097:1105	Several volatiles	1089:1105	Several volatiles belonging to different chemical classes	1089:1145	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	8	5	theme	volatiles	1272:1280	arg1	%					1252:1252	56.33%	1247:1252	56.33%	1247:1252	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	8	5	theme	volatiles	1272:1280	arg1	%					1263:1263	64.40%	1258:1263	64.40%	1258:1263	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	8	5	theme	volatiles	1272:1280	arg1	volatiles					1272:1280	all volatiles	1268:1280	all volatiles	1268:1280	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	10	6	theme	mg/mL	1659:1663	arg1	values					1643:1648	IC50 values	1638:1648	IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox	1638:1732	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	9	7	theme	leaf	1297:1300	arg1	extract					1302:1308	the leaf extract	1293:1308	the leaf extract	1293:1308	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	8	8	theme	different	1120:1128	arg1	classes					1139:1145	different chemical classes	1120:1145	different chemical classes	1120:1145	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	11	9	theme	functional	1815:1824	arg1	compounds					1826:1834	functional compounds	1815:1834	functional compounds that could act as antidiabetic and anti-obesogenic agents	1815:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	9	theme	functional	1815:1824	arg1	agents					1887:1892	antidiabetic and anti-obesogenic agents	1854:1892	agents	1887:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	10	10	dep	assay	1631:1635	arg1	values					1643:1648	IC50 values	1638:1648	IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox	1638:1732	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	7	11	theme	end-products	907:918	arg1	activity					993:1000	the antioxidant activity	977:1000	the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes	977:1078	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	11	theme	end-products	907:918	arg1	reference					963:971	reference	963:971	reference	963:971	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	11	theme	end-products	907:918	arg1	inhibition					874:883	The inhibition	870:883	The inhibition of advanced glycation end-products (AGEs)	870:925	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	3	12	theme	polyphenolic	258:269	arg1	profile					271:277	the polyphenolic profile	254:277	the polyphenolic profile	254:277	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	11	13	theme	antidiabetic	1854:1865	arg1	agents					1887:1892	antidiabetic and anti-obesogenic agents	1854:1892	agents	1887:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	13	theme	antidiabetic	1854:1865	arg1	compounds					1826:1834	functional compounds	1815:1834	functional compounds that could act as antidiabetic and anti-obesogenic agents	1815:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	9	14	theme	IC50	1323:1326	arg1	values					1328:1333	lower IC50 values	1317:1333	lower IC50 values	1317:1333	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	3	15	theme	cytotoxic	303:311	arg1	properties					313:322	the antioxidant and cytotoxic properties	283:322	the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts	283:376	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	5	16	dep	MATERIAL	531:538	arg1	characterization					565:580	The volatile characterization	552:580	The volatile characterization	552:580	MATERIAL AND METHODS The volatile characterization of the extracts was attained by HS-SPME-GC/MS analysis.
36219451	10	17	theme	leaf	1669:1672	arg1	extract					1674:1680	leaf extract	1669:1680	leaf extract	1669:1680	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	2	18	theme	available	196:204	arg1	information					206:216	very limited available information	183:216	very limited available information	183:216	(Brassicaceae) is an edible plant with very limited available information.
36219451	1	19	theme	CONTEXT	115:121	arg1	incana					132:137	CONTEXT Brassica incana Ten	115:141	CONTEXT Brassica incana Ten.	115:142	CONTEXT Brassica incana Ten.
36219451	5	20	theme	HS-SPME-GC/MS	614:626	arg1	analysis					628:635	HS-SPME-GC/MS analysis	614:635	HS-SPME-GC/MS analysis	614:635	MATERIAL AND METHODS The volatile characterization of the extracts was attained by HS-SPME-GC/MS analysis.
36219451	3	21	theme	antioxidant	287:297	arg1	properties					313:322	the antioxidant and cytotoxic properties	283:322	the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts	283:376	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	3	22	theme	leaf	331:334	arg1	profile					271:277	the polyphenolic profile	254:277	the polyphenolic profile	254:277	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	3	22	theme	leaf	331:334	arg1	properties					313:322	the antioxidant and cytotoxic properties	283:322	the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts	283:376	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	4	23	theme	potential	452:460	arg1	leaf					475:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf	379:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf	379:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	6	24	dep	investigated	686:697	arg1	in vitro					722:729	in vitro	722:729	was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs	682:867	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36219451	0	25	theme	incana	75:80	arg1	leaf					82:85	Brassica incana leaf	66:85	Brassica incana leaf	66:85	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	7	26	theme	comparative	1059:1069	arg1	purposes					1071:1078	comparative purposes	1059:1078	comparative purposes	1059:1078	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	3	27	theme	hydroalcoholic	354:367	arg1	extracts					369:376	the leaf and flowering top hydroalcoholic extracts	327:376	extracts	369:376	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	4	28	dep	composition	402:412	arg1	The					389:391	The	389:391	The	389:391	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	8	29	from	leaf	1214:1217	arg1	abundant					1197:1204	abundant	1197:1204	abundant	1197:1204	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	3	30	theme	flowering	340:348	arg1	extracts					369:376	the leaf and flowering top hydroalcoholic extracts	327:376	extracts	369:376	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	4	31	theme	flowering	484:492	arg1	extracts					498:505	flowering top extracts	484:505	flowering top extracts	484:505	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	0	32	theme	top	101:103	arg1	extracts					105:112	flowering top extracts	91:112	flowering top extracts	91:112	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	6	33	theme	pancreatic	758:767	arg1	lipase					769:774	pancreatic lipase	758:774	pancreatic lipase	758:774	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36219451	0	34	theme	Volatile	0:7	arg1	composition					9:19	Volatile composition	0:19	Volatile composition	0:19	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	7	35	theme	advanced	888:895	arg1	AGEs					921:924	AGEs	921:924	AGEs	921:924	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	35	theme	advanced	888:895	arg1	end-products					907:918	advanced glycation end-products	888:918	advanced glycation end-products (AGEs)	888:925	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	36	theme	xanthine/xanthine	1011:1027	arg1	system					1037:1042	the xanthine/xanthine oxidase system	1007:1042	the xanthine/xanthine oxidase system	1007:1042	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	0	37	theme	anti-obesity	40:51	arg1	potential					53:61	anti-obesity potential	40:61	anti-obesity potential	40:61	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	11	38	used	used	1796:1799	arg2	sources					1804:1810	sources	1804:1810	sources of functional compounds that could act as antidiabetic and anti-obesogenic agents	1804:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	38	used	used	1796:1799	arg2	tops					1769:1772	flowering tops	1759:1772	flowering tops from B. incana	1759:1787	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	38	used	used	1796:1799	arg2	CONCLUSIONS					1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	39	theme	anti-obesogenic	1871:1885	arg1	agents					1887:1892	antidiabetic and anti-obesogenic agents	1854:1892	agents	1887:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	39	theme	anti-obesogenic	1871:1885	arg1	compounds					1826:1834	functional compounds	1815:1834	functional compounds that could act as antidiabetic and anti-obesogenic agents	1815:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	8	40	theme	flowering	1223:1231	arg1	extracts					1237:1244	flowering top extracts	1223:1244	flowering top extracts (56.33% and 64.40% of all volatiles)	1223:1281	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	2	41	dep	plant	172:176	arg1	Brassicaceae					145:156	Brassicaceae	145:156	Brassicaceae	145:156	(Brassicaceae) is an edible plant with very limited available information.
36219451	10	42	theme	oxidase	1623:1629	arg1	assay					1631:1635	the xanthine oxidase assay	1610:1635	the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox)	1610:1733	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	11	43	theme	flowering	1759:1767	arg1	CONCLUSIONS					1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	43	theme	flowering	1759:1767	arg1	sources					1804:1810	sources	1804:1810	sources of functional compounds that could act as antidiabetic and anti-obesogenic agents	1804:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	43	theme	flowering	1759:1767	arg1	tops					1769:1772	flowering tops	1759:1772	flowering tops from B. incana	1759:1787	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	9	44	theme	superoxide	1457:1466	arg1	scavenging					1468:1477	superoxide scavenging	1457:1477	superoxide scavenging	1457:1477	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	2	45	with	plant	172:176	arg1	information					206:216	very limited available information	183:216	very limited available information	183:216	(Brassicaceae) is an edible plant with very limited available information.
36219451	3	46	theme	Previous	219:226	arg1	studies					228:234	Previous studies	219:234	Previous studies	219:234	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	4	47	theme	antidiabetic	422:433	arg1	potential					452:460	the antidiabetic and anti-obesity potential	418:460	the antidiabetic and anti-obesity potential of B. incana	418:473	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	4	48	theme	OBJECTIVE	379:387	arg1	leaf					475:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf	379:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf	379:478	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	6	49	theme	antidiabetic	642:653	arg1	potential					672:680	The antidiabetic and anti-obesity potential	638:680	The antidiabetic and anti-obesity potential	638:680	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36219451	9	50	theme	significant	1509:1519	arg1	differences					1521:1531	no statistically significant differences	1492:1531	no statistically significant differences between both samples	1492:1552	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	4	51	theme	volatile	393:400	arg1	composition					402:412	volatile composition	393:412	volatile composition	393:412	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	8	52	theme	chemical	1130:1137	arg1	classes					1139:1145	different chemical classes	1120:1145	different chemical classes	1120:1145	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	6	53	theme	reference	853:861	arg1	acarbose					841:848	acarbose	841:848	acarbose	841:848	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36219451	6	53	theme	reference	853:861	arg1	drugs					863:867	reference drugs	853:867	reference drugs	853:867	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36219451	6	53	theme	reference	853:861	arg1	orlistat					828:835	orlistat	828:835	orlistat	828:835	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36219451	4	54	theme	incana	468:473	arg1	potential					452:460	the antidiabetic and anti-obesity potential	418:460	the antidiabetic and anti-obesity potential of B. incana	418:473	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	4	54	theme	incana	468:473	arg1	composition					402:412	volatile composition	393:412	volatile composition	393:412	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	11	55	theme	compounds	1826:1834	arg1	CONCLUSIONS					1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	55	theme	compounds	1826:1834	arg1	tops					1769:1772	flowering tops	1759:1772	flowering tops from B. incana	1759:1787	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	55	theme	compounds	1826:1834	arg1	sources					1804:1810	sources	1804:1810	sources of functional compounds that could act as antidiabetic and anti-obesogenic agents	1804:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	10	56	dep	values	1643:1648	arg1	mg/mL					1689:1693	0.038 mg/mL	1683:1693	IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox	1638:1732	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	9	57	theme	lower	1317:1321	arg1	values					1328:1333	lower IC50 values	1317:1333	lower IC50 values	1317:1333	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	11	58	from	incana	1782:1787	arg1	CONCLUSIONS					1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS	1736:1746	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	58	from	incana	1782:1787	arg1	sources					1804:1810	sources	1804:1810	sources of functional compounds that could act as antidiabetic and anti-obesogenic agents	1804:1892	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	11	58	from	incana	1782:1787	arg1	tops					1769:1772	flowering tops	1759:1772	flowering tops from B. incana	1759:1787	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	4	59	theme	anti-obesity	439:450	arg1	potential					452:460	the antidiabetic and anti-obesity potential	418:460	the antidiabetic and anti-obesity potential of B. incana	418:473	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	5	60	theme	extracts	589:596	arg1	METHODS					544:550	METHODS	544:550	METHODS	544:550	MATERIAL AND METHODS The volatile characterization of the extracts was attained by HS-SPME-GC/MS analysis.
36219451	5	60	theme	extracts	589:596	arg1	MATERIAL					531:538	MATERIAL	531:538	MATERIAL	531:538	MATERIAL AND METHODS The volatile characterization of the extracts was attained by HS-SPME-GC/MS analysis.
36219451	1	61	theme	Brassica	123:130	arg1	incana					132:137	CONTEXT Brassica incana Ten	115:141	CONTEXT Brassica incana Ten.	115:142	CONTEXT Brassica incana Ten.
36219451	0	62	theme	Brassica	66:73	arg1	leaf					82:85	Brassica incana leaf	66:85	Brassica incana leaf	66:85	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	8	63	from	abundant	1197:1204	arg1	extracts					1237:1244	flowering top extracts	1223:1244	flowering top extracts (56.33% and 64.40% of all volatiles)	1223:1281	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	8	63	from	abundant	1197:1204	arg1	leaf					1214:1217	leaf	1214:1217	leaf	1214:1217	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	0	64	theme	leaf	82:85	arg1	composition					9:19	Volatile composition	0:19	Volatile composition	0:19	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	0	64	theme	leaf	82:85	arg1	potential					53:61	anti-obesity potential	40:61	anti-obesity potential	40:61	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	5	65	theme	volatile	556:563	arg1	characterization					565:580	The volatile characterization	552:580	The volatile characterization	552:580	MATERIAL AND METHODS The volatile characterization of the extracts was attained by HS-SPME-GC/MS analysis.
36219451	6	66	theme	anti-obesity	659:670	arg1	potential					672:680	The antidiabetic and anti-obesity potential	638:680	The antidiabetic and anti-obesity potential	638:680	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36219451	0	67	theme	flowering	91:99	arg1	extracts					105:112	flowering top extracts	91:112	flowering top extracts	91:112	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	3	68	theme	extracts	369:376	arg1	profile					271:277	the polyphenolic profile	254:277	the polyphenolic profile	254:277	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	3	68	theme	extracts	369:376	arg1	properties					313:322	the antioxidant and cytotoxic properties	283:322	the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts	283:376	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	4	69	theme	top	494:496	arg1	extracts					498:505	flowering top extracts	484:505	flowering top extracts	484:505	OBJECTIVE The volatile composition and the antidiabetic and anti-obesity potential of B. incana leaf and flowering top extracts have been investigated.
36219451	0	70	theme	extracts	105:112	arg1	composition					9:19	Volatile composition	0:19	Volatile composition	0:19	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	0	70	theme	extracts	105:112	arg1	potential					53:61	anti-obesity potential	40:61	anti-obesity potential	40:61	Volatile composition, antidiabetic, and anti-obesity potential of Brassica incana leaf and flowering top extracts.
36219451	8	71	theme	sulphur	1170:1176	arg1	compounds					1178:1186	sulphur compounds	1170:1186	sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles)	1170:1281	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	3	72	theme	top	350:352	arg1	extracts					369:376	the leaf and flowering top hydroalcoholic extracts	327:376	extracts	369:376	Previous studies have demonstrated the polyphenolic profile and the antioxidant and cytotoxic properties of the leaf and flowering top hydroalcoholic extracts.
36219451	9	73	dep	0.968	1358:1362	arg1	0.262 mg/mL					1409:1419	0.262 mg/mL	1409:1419	0.262 mg/mL	1409:1419	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	9	73	dep	0.968	1358:1362	arg1	0.192					1399:1403	0.192	1399:1403	0.192	1399:1403	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	9	73	dep	0.968	1358:1362	arg1	0.022					1431:1435	0.022	1431:1435	0.022	1431:1435	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	9	73	dep	0.968	1358:1362	arg1	0.038 mg/mL					1441:1451	0.038 mg/mL	1441:1451	0.038 mg/mL	1441:1451	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	8	74	dep	extracts	1237:1244	arg1	%					1252:1252	56.33%	1247:1252	56.33%	1247:1252	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	8	74	dep	extracts	1237:1244	arg1	%					1263:1263	64.40%	1258:1263	64.40%	1258:1263	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	8	74	dep	extracts	1237:1244	arg1	volatiles					1272:1280	all volatiles	1268:1280	all volatiles	1268:1280	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	7	75	theme	glycation	897:905	arg1	AGEs					921:924	AGEs	921:924	AGEs	921:924	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	75	theme	glycation	897:905	arg1	end-products					907:918	advanced glycation end-products	888:918	advanced glycation end-products (AGEs)	888:925	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	8	76	theme	abundant	1197:1204	arg1	compounds					1178:1186	sulphur compounds	1170:1186	sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles)	1170:1281	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	7	77	theme	oxidase	1029:1035	arg1	system					1037:1042	the xanthine/xanthine oxidase system	1007:1042	the xanthine/xanthine oxidase system	1007:1042	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	9	78	dep	assays	1350:1355	arg1	0.968					1358:1362	0.968	1358:1362	0.968	1358:1362	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	9	78	dep	assays	1350:1355	arg1	1.921 mg/mL					1368:1378	1.921 mg/mL	1368:1378	1.921 mg/mL	1368:1378	Although the leaf extract showed lower IC50 values in most of the assays (0.968 and 1.921 mg/mL for α-glucosidase; 0.192 and 0.262 mg/mL for AGEs; 0.022 and 0.038 mg/mL for superoxide scavenging), there were no statistically significant differences between both samples.
36219451	2	79	theme	edible	165:170	arg1	plant					172:176	an edible plant	162:176	an edible plant with very limited available information	162:216	(Brassicaceae) is an edible plant with very limited available information.
36219451	8	80	dep	RESULTS	1081:1087	arg1	identified					1152:1161	identified	1152:1161	identified	1152:1161	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	11	81	dep	CONCLUSIONS	1736:1746	arg1	Leaves					1748:1753	Leaves	1748:1753	Leaves	1748:1753	CONCLUSIONS Leaves and flowering tops from B. incana can be used as sources of functional compounds that could act as antidiabetic and anti-obesogenic agents.
36219451	8	82	from	extracts	1237:1244	arg1	abundant					1197:1204	abundant	1197:1204	abundant	1197:1204	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	2	83	theme	limited	188:194	arg1	information					206:216	very limited available information	183:216	very limited available information	183:216	(Brassicaceae) is an edible plant with very limited available information.
36219451	8	84	theme	top	1233:1235	arg1	extracts					1237:1244	flowering top extracts	1223:1244	flowering top extracts (56.33% and 64.40% of all volatiles)	1223:1281	RESULTS Several volatiles belonging to different chemical classes were identified, being sulphur compounds the most abundant in both leaf and flowering top extracts (56.33% and 64.40% of all volatiles).
36219451	10	85	theme	similar	1579:1585	arg1	behaviour					1587:1595	a similar behaviour	1577:1595	a similar behaviour to Trolox	1577:1605	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	7	86	theme	antioxidant	981:991	arg1	activity					993:1000	the antioxidant activity	977:1000	the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes	977:1078	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	7	86	theme	antioxidant	981:991	arg1	inhibition					874:883	The inhibition	870:883	The inhibition of advanced glycation end-products (AGEs)	870:925	The inhibition of advanced glycation end-products (AGEs) was measured with aminoguanidine as reference and the antioxidant activity with the xanthine/xanthine oxidase system and Trolox for comparative purposes.
36219451	10	87	theme	xanthine	1614:1621	arg1	oxidase					1623:1629	xanthine oxidase	1614:1629	the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox)	1610:1733	These extracts showed a similar behaviour to Trolox in the xanthine oxidase assay (IC50 values of 0.022 mg/mL for leaf extract; 0.038 mg/mL for flowering top and 0.028 for Trolox).
36219451	6	88	theme	different	797:805	arg1	concentrations					807:820	different concentrations	797:820	different concentrations using orlistat and acarbose as reference drugs	797:867	The antidiabetic and anti-obesity potential was investigated spectrophotometrically in vitro by the ability to modulate pancreatic lipase and α-glucosidase at different concentrations using orlistat and acarbose as reference drugs.
36781536	5	0	dep	markers	1001:1007	arg1	ITS					1010:1012	ITS	1010:1012	ITS	1010:1012	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	0	dep	markers	1001:1007	arg1	regions					1023:1029	EF1α regions	1018:1029	EF1α regions	1018:1029	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	1	theme	japonica	649:656	arg1	strains					658:664	Four Russian A. japonica strains	633:664	Four Russian A. japonica strains	633:664	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	2	theme	strain	1995:2000	arg1	MFP244011					2002:2010	strain MFP244011	1995:2010	strain MFP244011	1995:2010	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	10	3	located	detected	1845:1852	arg2	altenuene					1756:1764	altenuene	1756:1764	altenuene	1756:1764	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	3	located	detected	1845:1852	arg2	Mycotoxins					1682:1691	Mycotoxins	1682:1691	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene)	1682:1765	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	3	located	detected	1845:1852	arg2	alternariol					1694:1704	alternariol	1694:1704	alternariol	1694:1704	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	3	located	detected	1845:1852	arg2	tentoxin					1725:1732	tentoxin	1725:1732	tentoxin	1725:1732	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	3	located	detected	1845:1852	arg1	cultures					1857:1864	cultures	1857:1864	cultures of the A. japonica strains	1857:1891	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	3	located	detected	1845:1852	arg2	ether					1718:1722	its methyl ether	1707:1722	its methyl ether	1707:1722	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	3	located	detected	1845:1852	arg2	acid					1746:1749	tenuazonic acid	1735:1749	tenuazonic acid	1735:1749	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	3	4	from	data	297:300	arg1	metabolites					315:325	its toxic metabolites	305:325	its toxic metabolites	305:325	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	6	5	theme	substance	1104:1112	arg1	yields					1114:1119	Biomass and extractive substance yields	1081:1119	yields	1114:1119	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	9	6	dep	phomenins	1561:1569	arg1	phomenins					1561:1569	phomenins A and B	1561:1577	phomenins A and B	1561:1577	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	9	6	dep	phomenins	1561:1569	arg1	B					1577:1577	B	1577:1577	B	1577:1577	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	9	6	dep	phomenins	1561:1569	arg1	A					1571:1571	A	1571:1571	A	1571:1571	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	2	7	theme	Alternaria	160:169	arg1	japonica					171:178	Alternaria japonica	160:178	Alternaria japonica	160:178	Alternaria japonica is pathogenic to small radish and certain other crucifers, but has not been studied in sufficient detail.
36781536	5	8	dep	characters	754:763	arg1	morphology					783:792	morphology	783:792	morphology	783:792	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	8	dep	characters	754:763	arg1	characters					754:763	morphological characters	740:763	morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week)	740:910	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	8	dep	characters	754:763	arg1	diameter					770:777	the diameter	766:777	the diameter	766:777	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	10	9	theme	A.	1873:1874	arg1	strains					1885:1891	the A. japonica strains	1869:1891	the A. japonica strains	1869:1891	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	5	10	theme	Russian	638:644	arg1	strains					658:664	Four Russian A. japonica strains	633:664	Four Russian A. japonica strains	633:664	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	10	11	theme	methyl	1711:1716	arg1	Mycotoxins					1682:1691	Mycotoxins	1682:1691	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene)	1682:1765	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	11	theme	methyl	1711:1716	arg1	ether					1718:1722	its methyl ether	1707:1722	its methyl ether	1707:1722	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	5	12	theme	yeast	865:869	arg1	agar					893:896	the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar	815:896	agar	893:896	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	3	13	theme	Discrepant	286:295	arg1	data					297:300	Discrepant data	286:300	Discrepant data on its toxic metabolites	286:325	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	3	14	from	literature	348:357	arg1	available					331:339	available	331:339	available	331:339	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	11	15	theme	extractive	1928:1937	arg1	substances					1939:1948	extractive substances	1928:1948	extractive substances	1928:1948	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	5	16	theme	carrot	843:848	arg1	PCA					856:858	the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar	815:896	PCA	856:858	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	8	17	theme	small	1393:1397	arg1	radish					1399:1404	small radish leaves	1393:1411	small radish leaves	1393:1411	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	5	18	theme	EF1α	1018:1021	arg1	regions					1023:1029	EF1α regions	1018:1029	EF1α regions	1018:1029	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	3	19	theme	toxic	309:313	arg1	metabolites					315:325	its toxic metabolites	305:325	its toxic metabolites	305:325	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	11	20	theme	japonica	1973:1980	arg1	strains					1982:1988	the A. japonica strains	1966:1988	the A. japonica strains	1966:1988	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	10	21	theme	strains	1885:1891	arg1	cultures					1857:1864	cultures	1857:1864	cultures of the A. japonica strains	1857:1891	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	3	22	from	available	331:339	arg1	literature					348:357	the literature	344:357	the literature	344:357	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	9	23	theme	japonica	1663:1670	arg1	extracts					1672:1679	A. japonica extracts	1660:1679	A. japonica extracts	1660:1679	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	7	24	theme	metabolite	1289:1298	arg1	profiling					1300:1308	metabolite profiling	1289:1308	metabolite profiling by HPLC/MS-UV	1289:1322	Minor differences between the A. japonica strains were detected via metabolite profiling by HPLC/MS-UV.
36781536	5	25	theme	multifaceted	690:701	arg1	approach					703:710	a multifaceted approach	688:710	a multifaceted approach	688:710	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	26	theme	markers	1001:1007	arg1	analyses					728:735	analyses	728:735	analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores)	728:966	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	26	theme	markers	1001:1007	arg1	production					1068:1077	mycotoxin production	1058:1077	mycotoxin production	1058:1077	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	26	theme	markers	1001:1007	arg1	data					1052:1055	chemotaxonomic data	1037:1055	chemotaxonomic data (mycotoxin production)	1037:1078	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	26	theme	markers	1001:1007	arg1	sequences					984:992	the nucleotide sequences	969:992	the nucleotide sequences of DNA markers (ITS and EF1α regions)	969:1030	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	27	theme	Extract	1894:1900	arg1	toxicity					1902:1909	Extract toxicity	1894:1909	Extract toxicity	1894:1909	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	1	28	theme	compounds	149:157	arg1	Fungi					82:86	Fungi	82:86	Fungi of the genus Alternaria	82:110	Fungi of the genus Alternaria are producers of biologically active compounds.
36781536	1	28	theme	compounds	149:157	arg1	producers					116:124	producers	116:124	producers of biologically active compounds	116:157	Fungi of the genus Alternaria are producers of biologically active compounds.
36781536	8	29	theme	Paramecium	1437:1446	arg1	caudatum					1448:1455	Paramecium caudatum	1437:1455	Paramecium caudatum	1437:1455	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	6	30	theme	japonica	1127:1134	arg1	cultures					1136:1143	A. japonica cultures	1124:1143	A. japonica cultures	1124:1143	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	10	31	dep	Mycotoxins	1682:1691	arg1	acid					1746:1749	tenuazonic acid	1735:1749	tenuazonic acid	1735:1749	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	31	dep	Mycotoxins	1682:1691	arg1	ether					1718:1722	its methyl ether	1707:1722	its methyl ether	1707:1722	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	31	dep	Mycotoxins	1682:1691	arg1	Mycotoxins					1682:1691	Mycotoxins	1682:1691	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene)	1682:1765	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	31	dep	Mycotoxins	1682:1691	arg1	altenuene					1756:1764	altenuene	1756:1764	altenuene	1756:1764	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	31	dep	Mycotoxins	1682:1691	arg1	alternariol					1694:1704	alternariol	1694:1704	alternariol	1694:1704	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	31	dep	Mycotoxins	1682:1691	arg1	tentoxin					1725:1732	tentoxin	1725:1732	tentoxin	1725:1732	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	0	32	theme	Toxicological	19:31	arg1	Characterization					33:48	Toxicological Characterization	19:48	Toxicological Characterization	19:48	Identification and Toxicological Characterization of Alternaria japonica Strains.
36781536	5	33	theme	mycotoxin	1058:1066	arg1	data					1052:1055	chemotaxonomic data	1037:1055	chemotaxonomic data (mycotoxin production)	1037:1078	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	33	theme	mycotoxin	1058:1066	arg1	production					1068:1077	mycotoxin production	1058:1077	mycotoxin production	1058:1077	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	34	theme	synthetic	2104:2112	arg1	medium					2114:2119	the M1D synthetic medium	2096:2119	the M1D synthetic medium	2096:2119	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	8	35	with	comparable	1468:1477	arg1	that					1484:1487	that	1484:1487	that	1484:1487	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	5	36	theme	characters	754:763	arg1	analyses					728:735	analyses	728:735	analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores)	728:966	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	36	theme	characters	754:763	arg1	production					1068:1077	mycotoxin production	1058:1077	mycotoxin production	1058:1077	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	36	theme	characters	754:763	arg1	data					1052:1055	chemotaxonomic data	1037:1055	chemotaxonomic data (mycotoxin production)	1037:1078	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	36	theme	characters	754:763	arg1	sequences					984:992	the nucleotide sequences	969:992	the nucleotide sequences of DNA markers (ITS and EF1α regions)	969:1030	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	9	37	theme	Brassicicolin	1516:1528	arg1	A					1530:1530	Brassicicolin A	1516:1530	Brassicicolin A	1516:1530	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	1	38	theme	Alternaria	101:110	arg1	Fungi					82:86	Fungi	82:86	Fungi of the genus Alternaria	82:110	Fungi of the genus Alternaria are producers of biologically active compounds.
36781536	1	38	theme	Alternaria	101:110	arg1	producers					116:124	producers	116:124	producers of biologically active compounds	116:157	Fungi of the genus Alternaria are producers of biologically active compounds.
36781536	7	39	theme	japonica	1254:1261	arg1	strains					1263:1269	the A. japonica strains	1247:1269	the A. japonica strains	1247:1269	Minor differences between the A. japonica strains were detected via metabolite profiling by HPLC/MS-UV.
36781536	11	40	theme	known	2046:2050	arg1	producer					2034:2041	a producer	2032:2041	a producer of known and, presumably, new toxins	2032:2078	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	8	41	theme	tenuissima	1495:1504	arg1	extracts					1506:1513	A. tenuissima extracts	1492:1513	A. tenuissima extracts	1492:1513	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	5	42	theme	media	830:834	arg1	PCA					856:858	the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar	815:896	PCA	856:858	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	43	theme	conidial	917:924	arg1	size					926:929	the conidial size	913:929	the conidial size	913:929	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	44	theme	toxins	2073:2078	arg1	producer					2034:2041	a producer	2032:2041	a producer of known and, presumably, new toxins	2032:2078	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	4	45	theme	A.	562:563	arg1	strains					574:580	the Russian A. japonica strains	550:580	the Russian A. japonica strains	550:580	The objectives of this study were to accurately identify the Russian A. japonica strains and to assess the A. japonica toxigenic potential.
36781536	2	46	theme	small	197:201	arg1	radish					203:208	small radish	197:208	small radish	197:208	Alternaria japonica is pathogenic to small radish and certain other crucifers, but has not been studied in sufficient detail.
36781536	8	47	theme	A.	1337:1338	arg1	cultures					1349:1356	A. japonica cultures	1337:1356	A. japonica cultures	1337:1356	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	3	48	theme	nutritive	394:402	arg1	substrates					404:413	nutritive substrates	394:413	nutritive substrates	394:413	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	8	49	theme	cultures	1349:1356	arg1	Extracts					1325:1332	Extracts	1325:1332	Extracts of A. japonica cultures	1325:1356	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	0	50	theme	japonica	64:71	arg1	Strains					73:79	Alternaria japonica Strains	53:79	Alternaria japonica Strains	53:79	Identification and Toxicological Characterization of Alternaria japonica Strains.
36781536	8	51	dep	radish	1399:1404	arg1	leaves					1406:1411	leaves	1406:1411	leaves	1406:1411	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	2	52	theme	other	222:226	arg1	crucifers					228:236	certain other crucifers	214:236	certain other crucifers	214:236	Alternaria japonica is pathogenic to small radish and certain other crucifers, but has not been studied in sufficient detail.
36781536	8	53	theme	phytotoxic	1366:1375	arg1	activity					1377:1384	phytotoxic activity	1366:1384	phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum	1366:1455	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	0	54	theme	Strains	73:79	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and Toxicological Characterization of Alternaria japonica Strains.
36781536	0	54	theme	Strains	73:79	arg1	Characterization					33:48	Toxicological Characterization	19:48	Toxicological Characterization	19:48	Identification and Toxicological Characterization of Alternaria japonica Strains.
36781536	4	55	theme	japonica	603:610	arg1	potential					622:630	the A. japonica toxigenic potential	596:630	the A. japonica toxigenic potential	596:630	The objectives of this study were to accurately identify the Russian A. japonica strains and to assess the A. japonica toxigenic potential.
36781536	5	56	theme	A.	646:647	arg1	strains					658:664	Four Russian A. japonica strains	633:664	Four Russian A. japonica strains	633:664	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	57	dep	medium	2172:2177	arg1	e.g.					2152:2155	e.g.	2152:2155	e.g.	2152:2155	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	10	58	theme	species	1813:1819	arg1	characteristic					1778:1791	characteristic	1778:1791	characteristic	1778:1791	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	6	59	theme	liquid	1206:1211	arg1	medium					1213:1218	the liquid medium	1202:1218	the liquid medium	1202:1218	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	9	60	theme	several	1600:1606	arg1	species					1608:1614	several species	1600:1614	several species of the genus Alternaria	1600:1638	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	11	61	theme	new	2069:2071	arg1	toxins					2073:2078	new toxins	2069:2078	new toxins	2069:2078	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	6	62	theme	extractive	1093:1102	arg1	yields					1114:1119	Biomass and extractive substance yields	1081:1119	yields	1114:1119	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	11	63	theme	liquid	2138:2143	arg1	media					2145:2149	semisynthetic liquid media	2124:2149	semisynthetic liquid media (e.g., the Sabouraud medium)	2124:2178	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	11	64	theme	substances	1939:1948	arg1	toxicity					1902:1909	Extract toxicity	1894:1909	Extract toxicity	1894:1909	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	11	64	theme	substances	1939:1948	arg1	yield					1919:1923	the yield	1915:1923	the yield of extractive substances	1915:1948	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	8	65	theme	comparable	1468:1477	arg1	level					1462:1466	a level	1460:1466	a level comparable with that of A. tenuissima extracts	1460:1513	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	9	66	theme	genus	1623:1627	arg1	Alternaria					1629:1638	the genus Alternaria	1619:1638	the genus Alternaria	1619:1638	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	6	67	theme	Biomass	1081:1087	arg1	yields					1114:1119	Biomass and extractive substance yields	1081:1119	yields	1114:1119	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	11	68	theme	Sabouraud	2162:2170	arg1	medium					2172:2177	the Sabouraud medium	2158:2177	the Sabouraud medium	2158:2177	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	5	69	theme	potato	836:841	arg1	PCA					856:858	the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar	815:896	PCA	856:858	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	70	theme	A.	1970:1971	arg1	strains					1982:1988	the A. japonica strains	1966:1988	the A. japonica strains	1966:1988	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	10	71	theme	tenuazonic	1735:1744	arg1	Mycotoxins					1682:1691	Mycotoxins	1682:1691	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene)	1682:1765	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	10	71	theme	tenuazonic	1735:1744	arg1	acid					1746:1749	tenuazonic acid	1735:1749	tenuazonic acid	1735:1749	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	5	72	theme	agar	850:853	arg1	PCA					856:858	the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar	815:896	PCA	856:858	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	3	73	used	used	419:422	arg2	substrates					404:413	nutritive substrates	394:413	nutritive substrates	394:413	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	3	73	used	used	419:422	arg2	set					387:389	a limited set	377:389	a limited set of nutritive substrates	377:413	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	9	74	theme	A.	1660:1661	arg1	extracts					1672:1679	A. japonica extracts	1660:1679	A. japonica extracts	1660:1679	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	10	75	theme	japonica	1876:1883	arg1	strains					1885:1891	the A. japonica strains	1869:1891	the A. japonica strains	1869:1891	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	5	76	theme	DNA	997:999	arg1	markers					1001:1007	DNA markers	997:1007	DNA markers (ITS and EF1α regions)	997:1030	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	2	77	theme	sufficient	267:276	arg1	detail					278:283	sufficient detail	267:283	sufficient detail	267:283	Alternaria japonica is pathogenic to small radish and certain other crucifers, but has not been studied in sufficient detail.
36781536	1	78	theme	active	142:147	arg1	compounds					149:157	biologically active compounds	129:157	biologically active compounds	129:157	Fungi of the genus Alternaria are producers of biologically active compounds.
36781536	6	79	theme	cultures	1136:1143	arg1	yields					1114:1119	Biomass and extractive substance yields	1081:1119	yields	1114:1119	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	5	80	theme	extract-glucose	871:885	arg1	agar					893:896	the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar	815:896	agar	893:896	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	81	theme	M1D	2100:2102	arg1	medium					2114:2119	the M1D synthetic medium	2096:2119	the M1D synthetic medium	2096:2119	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	6	82	theme	A.	1124:1125	arg1	cultures					1136:1143	A. japonica cultures	1124:1143	A. japonica cultures	1124:1143	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	5	83	theme	YES	888:890	arg1	agar					893:896	the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar	815:896	agar	893:896	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	4	84	theme	study	516:520	arg1	objectives					497:506	The objectives	493:506	The objectives of this study	493:520	The objectives of this study were to accurately identify the Russian A. japonica strains and to assess the A. japonica toxigenic potential.
36781536	5	85	theme	size	926:929	arg1	analyses					728:735	analyses	728:735	analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores)	728:966	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	85	theme	size	926:929	arg1	production					1068:1077	mycotoxin production	1058:1077	mycotoxin production	1058:1077	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	85	theme	size	926:929	arg1	data					1052:1055	chemotaxonomic data	1037:1055	chemotaxonomic data (mycotoxin production)	1037:1078	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	85	theme	size	926:929	arg1	sequences					984:992	the nucleotide sequences	969:992	the nucleotide sequences of DNA markers (ITS and EF1α regions)	969:1030	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	11	86	theme	semisynthetic	2124:2136	arg1	media					2145:2149	semisynthetic liquid media	2124:2149	semisynthetic liquid media (e.g., the Sabouraud medium)	2124:2178	Extract toxicity and the yield of extractive substances were studied in the A. japonica strains, and strain MFP244011 proved promising as a producer of known and, presumably, new toxins upon culture on the M1D synthetic medium or semisynthetic liquid media (e.g., the Sabouraud medium).
36781536	5	87	theme	morphological	740:752	arg1	morphology					783:792	morphology	783:792	morphology	783:792	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	87	theme	morphological	740:752	arg1	characters					754:763	morphological characters	740:763	morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week)	740:910	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	87	theme	morphological	740:752	arg1	diameter					770:777	the diameter	766:777	the diameter	766:777	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	8	88	theme	A.	1492:1493	arg1	extracts					1506:1513	A. tenuissima extracts	1492:1513	A. tenuissima extracts	1492:1513	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	3	89	theme	species	440:446	arg1	identification					448:461	species identification	440:461	species identification of the strains	440:476	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	5	90	theme	chemotaxonomic	1037:1050	arg1	data					1052:1055	chemotaxonomic data	1037:1055	chemotaxonomic data (mycotoxin production)	1037:1078	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	90	theme	chemotaxonomic	1037:1050	arg1	production					1068:1077	mycotoxin production	1058:1077	mycotoxin production	1058:1077	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	1	91	theme	genus	95:99	arg1	Alternaria					101:110	the genus Alternaria	91:110	the genus Alternaria	91:110	Fungi of the genus Alternaria are producers of biologically active compounds.
36781536	7	92	theme	A.	1251:1252	arg1	strains					1263:1269	the A. japonica strains	1247:1269	the A. japonica strains	1247:1269	Minor differences between the A. japonica strains were detected via metabolite profiling by HPLC/MS-UV.
36781536	4	93	theme	Russian	554:560	arg1	strains					574:580	the Russian A. japonica strains	550:580	the Russian A. japonica strains	550:580	The objectives of this study were to accurately identify the Russian A. japonica strains and to assess the A. japonica toxigenic potential.
36781536	5	94	theme	diagnostic	819:828	arg1	media					830:834	the diagnostic media	815:834	the diagnostic media	815:834	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	9	95	theme	dihydrobrassicicolin	1533:1552	arg1	A					1554:1554	dihydrobrassicicolin A	1533:1554	dihydrobrassicicolin A	1533:1554	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	3	96	theme	limited	379:385	arg1	set					387:389	a limited set	377:389	a limited set of nutritive substrates	377:413	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	3	96	theme	limited	379:385	arg1	substrates					404:413	nutritive substrates	394:413	nutritive substrates	394:413	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	4	97	theme	japonica	565:572	arg1	strains					574:580	the Russian A. japonica strains	550:580	the Russian A. japonica strains	550:580	The objectives of this study were to accurately identify the Russian A. japonica strains and to assess the A. japonica toxigenic potential.
36781536	3	98	theme	strains	470:476	arg1	identification					448:461	species identification	440:461	species identification of the strains	440:476	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	0	99	theme	Alternaria	53:62	arg1	Strains					73:79	Alternaria japonica Strains	53:79	Alternaria japonica Strains	53:79	Identification and Toxicological Characterization of Alternaria japonica Strains.
36781536	7	100	theme	Minor	1221:1225	arg1	differences					1227:1237	Minor differences	1221:1237	Minor differences between the A. japonica strains	1221:1269	Minor differences between the A. japonica strains were detected via metabolite profiling by HPLC/MS-UV.
36781536	5	101	theme	colonies	797:804	arg1	morphology					783:792	morphology	783:792	morphology	783:792	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	101	theme	colonies	797:804	arg1	characters					754:763	morphological characters	740:763	morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week)	740:910	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	101	theme	colonies	797:804	arg1	diameter					770:777	the diameter	766:777	the diameter	766:777	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	102	theme	nucleotide	973:982	arg1	sequences					984:992	the nucleotide sequences	969:992	the nucleotide sequences of DNA markers (ITS and EF1α regions)	969:1030	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	3	103	theme	substrates	404:413	arg1	set					387:389	a limited set	377:389	a limited set of nutritive substrates	377:413	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	3	103	theme	substrates	404:413	arg1	substrates					404:413	nutritive substrates	394:413	nutritive substrates	394:413	Discrepant data on its toxic metabolites are available in the literature, possibly because a limited set of nutritive substrates was used in culturing or species identification of the strains was incorrect.
36781536	10	104	theme	cosmopolitan	1800:1811	arg1	species					1813:1819	the cosmopolitan species	1796:1819	the cosmopolitan species	1796:1819	Mycotoxins (alternariol, its methyl ether, tentoxin, tenuazonic acid, and altenuene), which are characteristic of the cosmopolitan species A. tenuissima, were not detected in cultures of the A. japonica strains.
36781536	8	105	theme	japonica	1340:1347	arg1	cultures					1349:1356	A. japonica cultures	1337:1356	A. japonica cultures	1337:1356	Extracts of A. japonica cultures exerted phytotoxic activity toward small radish leaves and cytotoxicity toward Paramecium caudatum to a level comparable with that of A. tenuissima extracts.
36781536	9	106	theme	Alternaria	1629:1638	arg1	species					1608:1614	several species	1600:1614	several species of the genus Alternaria	1600:1638	Brassicicolin A, dihydrobrassicicolin A, and phomenins A and B, which are known for several species of the genus Alternaria, were identified in A. japonica extracts.
36781536	6	107	theme	medium	1213:1218	arg1	composition					1187:1197	the composition	1183:1197	the composition of the liquid medium	1183:1218	Biomass and extractive substance yields of A. japonica cultures were found to significantly depend on the composition of the liquid medium.
36781536	5	108	theme	presence	940:947	arg1	analyses					728:735	analyses	728:735	analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores)	728:966	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	108	theme	presence	940:947	arg1	production					1068:1077	mycotoxin production	1058:1077	mycotoxin production	1058:1077	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	108	theme	presence	940:947	arg1	data					1052:1055	chemotaxonomic data	1037:1055	chemotaxonomic data (mycotoxin production)	1037:1078	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	108	theme	presence	940:947	arg1	sequences					984:992	the nucleotide sequences	969:992	the nucleotide sequences of DNA markers (ITS and EF1α regions)	969:1030	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	4	109	theme	A.	600:601	arg1	potential					622:630	the A. japonica toxigenic potential	596:630	the A. japonica toxigenic potential	596:630	The objectives of this study were to accurately identify the Russian A. japonica strains and to assess the A. japonica toxigenic potential.
36781536	2	110	theme	certain	214:220	arg1	crucifers					228:236	certain other crucifers	214:236	certain other crucifers	214:236	Alternaria japonica is pathogenic to small radish and certain other crucifers, but has not been studied in sufficient detail.
36781536	5	111	theme	chlamydospores	952:965	arg1	presence					940:947	the presence	936:947	the presence of chlamydospores	936:965	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	111	theme	chlamydospores	952:965	arg1	characters					754:763	morphological characters	740:763	morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week)	740:910	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	111	theme	chlamydospores	952:965	arg1	diameter					770:777	the diameter	766:777	the diameter	766:777	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	111	theme	chlamydospores	952:965	arg1	morphology					783:792	morphology	783:792	morphology	783:792	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	5	111	theme	chlamydospores	952:965	arg1	size					926:929	the conidial size	913:929	the conidial size	913:929	Four Russian A. japonica strains were identified using a multifaceted approach, which included analyses of morphological characters (the diameter and morphology of colonies grown on the diagnostic media potato carrot agar (PCA) and yeast extract-glucose (YES) agar for one week), the conidial size, and the presence of chlamydospores), the nucleotide sequences of DNA markers (ITS and EF1α regions), and chemotaxonomic data (mycotoxin production).
36781536	4	112	theme	toxigenic	612:620	arg1	potential					622:630	the A. japonica toxigenic potential	596:630	the A. japonica toxigenic potential	596:630	The objectives of this study were to accurately identify the Russian A. japonica strains and to assess the A. japonica toxigenic potential.
35816111	7	0	theme	biological	1217:1226	arg1	functions					1228:1236	their biological functions	1211:1236	their biological functions	1211:1236	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	6	1	theme	bacteria	906:913	arg1	abundance					878:886	the abundance	874:886	the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium	874:960	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	1	2	theme	major	161:165	arg1	problem					174:180	a major health problem worldwide	159:190	a major health problem worldwide	159:190	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	6	3	theme	harmful	998:1004	arg1	Collinsella					1051:1061	Collinsella	1051:1061	Collinsella	1051:1061	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	3	theme	harmful	998:1004	arg1	Rikenella					1015:1023	Rikenella	1015:1023	Rikenella	1015:1023	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	3	theme	harmful	998:1004	arg1	Megasphaera					1067:1077	Megasphaera	1067:1077	Megasphaera	1067:1077	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	3	theme	harmful	998:1004	arg1	bacteria					1006:1013	the harmful bacteria	994:1013	the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice	994:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	0	4	theme	metabolomic	82:92	arg1	profiles					94:101	serum metabolomic profiles	76:101	serum metabolomic profiles in high-fat diet-induced obese mice	76:137	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	6	5	theme	beneficial	895:904	arg1	Alistipes					936:944	Alistipes	936:944	Alistipes	936:944	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	5	theme	beneficial	895:904	arg1	bacteria					906:913	the beneficial bacteria	891:913	the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium	891:960	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	5	theme	beneficial	895:904	arg1	Clostridium					950:960	Clostridium	950:960	Clostridium	950:960	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	5	theme	beneficial	895:904	arg1	Muribaculaceae					915:928	Muribaculaceae	915:928	Muribaculaceae	915:928	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	6	from	bacteria	1006:1013	arg1	mice					1086:1089	HFD mice	1082:1089	HFD mice	1082:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	9	7	theme	HFD-induced	1485:1495	arg1	dysbiosis					1501:1509	HFD-induced gut dysbiosis	1485:1509	HFD-induced gut dysbiosis	1485:1509	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	1	8	theme	health	167:172	arg1	problem					174:180	a major health problem worldwide	159:190	a major health problem worldwide	159:190	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	9	9	theme	novel	1443:1447	arg1	modulator					1464:1472	a novel gut microbiota modulator	1441:1472	a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis	1441:1509	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	9	9	theme	novel	1443:1447	arg1	It					1423:1424	It	1423:1424	It	1423:1424	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	0	10	theme	high-fat	106:113	arg1	mice					134:137	high-fat diet-induced obese mice	106:137	high-fat diet-induced obese mice	106:137	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	6	11	theme	gut	760:762	arg1	analysis					775:782	gut microbiota analysis	760:782	gut microbiota analysis	760:782	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	7	12	theme	non-targeted	1107:1118	arg1	analysis					1133:1140	serum non-targeted metabolomics analysis	1101:1140	serum non-targeted metabolomics analysis	1101:1140	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	2	13	theme	pilosula	301:308	arg1	CPOs					328:331	CPOs	328:331	CPOs	328:331	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	2	13	theme	pilosula	301:308	arg1	oligosaccharides					310:325	Codonopsis pilosula oligosaccharides	290:325	Codonopsis pilosula oligosaccharides (CPOs)	290:332	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	6	14	dep	Muribaculaceae	915:928	arg1	spp.					930:933	Muribaculaceae spp.	915:933	Muribaculaceae spp.	915:933	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	7	15	theme	bile	1276:1279	arg1	metabolism					1286:1295	bile acid metabolism	1276:1295	bile acid metabolism	1276:1295	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	3	16	theme	mg	520:521	arg1	CPOs					510:513	CPOs	510:513	CPOs (500 mg kg-1)	510:527	Male C57BL/6J mice were fed with a HFD for 16 weeks and treated daily with CPOs (500 mg kg-1).
35816111	3	16	theme	mg	520:521	arg1	kg-1					523:526	500 mg kg-1	516:526	500 mg kg-1	516:526	Male C57BL/6J mice were fed with a HFD for 16 weeks and treated daily with CPOs (500 mg kg-1).
35816111	6	17	from	mice	1086:1089	arg1	abundance					981:989	the abundance	977:989	the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice	977:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	2	18	theme	Codonopsis	290:299	arg1	CPOs					328:331	CPOs	328:331	CPOs	328:331	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	2	18	theme	Codonopsis	290:299	arg1	oligosaccharides					310:325	Codonopsis pilosula oligosaccharides	290:325	Codonopsis pilosula oligosaccharides (CPOs)	290:332	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	4	19	theme	body	560:563	arg1	weight					565:570	body weight	560:570	body weight	560:570	CPO supplementation decreased body weight and fat accumulation and improved glucose tolerance in HFD-fed mice.
35816111	0	20	theme	obese	128:132	arg1	mice					134:137	high-fat diet-induced obese mice	106:137	high-fat diet-induced obese mice	106:137	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	6	21	dep	bacteria	1006:1013	arg1	Collinsella					1051:1061	Collinsella	1051:1061	Collinsella	1051:1061	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	21	dep	bacteria	1006:1013	arg1	Rikenella					1015:1023	Rikenella	1015:1023	Rikenella	1015:1023	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	21	dep	bacteria	1006:1013	arg1	spp.					1045:1048	Enterobacteriaceae spp.	1026:1048	Enterobacteriaceae spp.	1026:1048	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	21	dep	bacteria	1006:1013	arg1	bacteria					1006:1013	the harmful bacteria	994:1013	the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice	994:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	21	dep	bacteria	1006:1013	arg1	Megasphaera					1067:1077	Megasphaera	1067:1077	Megasphaera	1067:1077	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	2	22	theme	obese	423:427	arg1	mice					429:432	high-fat diet (HFD) induced obese mice	395:432	high-fat diet (HFD) induced obese mice	395:432	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	8	23	theme	HFD-induced	1362:1372	arg1	obesity					1374:1380	HFD-induced obesity	1362:1380	HFD-induced obesity	1362:1380	The results demonstrate that CPO supplementation can ameliorate HFD-induced obesity and obesity-related metabolic disorders.
35816111	3	24	theme	C57BL/6J	440:447	arg1	mice					449:452	Male C57BL/6J mice	435:452	Male C57BL/6J mice	435:452	Male C57BL/6J mice were fed with a HFD for 16 weeks and treated daily with CPOs (500 mg kg-1).
35816111	0	25	theme	diet-induced	115:126	arg1	mice					134:137	high-fat diet-induced obese mice	106:137	high-fat diet-induced obese mice	106:137	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	9	26	used	used	1433:1436	arg2	It					1423:1424	It	1423:1424	It	1423:1424	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	9	26	used	used	1433:1436	arg2	modulator					1464:1472	a novel gut microbiota modulator	1441:1472	a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis	1441:1509	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	6	27	from	abundance	981:989	arg1	mice					1086:1089	HFD mice	1082:1089	HFD mice	1082:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	28	theme	microbiota	764:773	arg1	analysis					775:782	gut microbiota analysis	760:782	gut microbiota analysis	760:782	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	5	29	from	effects	664:670	arg1	infiltration					731:742	improved macrophage infiltration	711:742	improved macrophage infiltration	711:742	CPOs also reversed the effects of the HFD on inflammatory markers and improved macrophage infiltration.
35816111	5	29	from	effects	664:670	arg1	markers					699:705	inflammatory markers	686:705	inflammatory markers	686:705	CPOs also reversed the effects of the HFD on inflammatory markers and improved macrophage infiltration.
35816111	1	30	theme	worldwide	182:190	arg1	problem					174:180	a major health problem worldwide	159:190	a major health problem worldwide	159:190	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	0	31	theme	pilosula	11:18	arg1	oligosaccharides					20:35	Codonopsis pilosula oligosaccharides	0:35	Codonopsis pilosula oligosaccharides	0:35	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	5	32	theme	macrophage	720:729	arg1	infiltration					731:742	improved macrophage infiltration	711:742	improved macrophage infiltration	711:742	CPOs also reversed the effects of the HFD on inflammatory markers and improved macrophage infiltration.
35816111	0	33	theme	Codonopsis	0:9	arg1	oligosaccharides					20:35	Codonopsis pilosula oligosaccharides	0:35	Codonopsis pilosula oligosaccharides	0:35	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	8	34	theme	metabolic	1402:1410	arg1	disorders					1412:1420	obesity-related metabolic disorders	1386:1420	obesity-related metabolic disorders	1386:1420	The results demonstrate that CPO supplementation can ameliorate HFD-induced obesity and obesity-related metabolic disorders.
35816111	2	35	theme	high-fat	395:402	arg1	HFD					410:412	HFD	410:412	HFD	410:412	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	2	35	theme	high-fat	395:402	arg1	diet					404:407	high-fat diet	395:407	high-fat diet (HFD)	395:413	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	6	36	theme	HFD	1082:1084	arg1	mice					1086:1089	HFD mice	1082:1089	HFD mice	1082:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	7	37	theme	metabolomics	1120:1131	arg1	analysis					1133:1140	serum non-targeted metabolomics analysis	1101:1140	serum non-targeted metabolomics analysis	1101:1140	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	2	38	from	obesity	348:354	arg1	mice					429:432	high-fat diet (HFD) induced obese mice	395:432	high-fat diet (HFD) induced obese mice	395:432	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	4	39	from	tolerance	614:622	arg1	mice					635:638	HFD-fed mice	627:638	HFD-fed mice	627:638	CPO supplementation decreased body weight and fat accumulation and improved glucose tolerance in HFD-fed mice.
35816111	4	40	theme	glucose	606:612	arg1	tolerance					614:622	glucose tolerance	606:622	glucose tolerance in HFD-fed mice	606:638	CPO supplementation decreased body weight and fat accumulation and improved glucose tolerance in HFD-fed mice.
35816111	7	41	theme	CPO	1176:1178	arg1	treatment					1180:1188	CPO treatment	1176:1188	CPO treatment	1176:1188	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	4	42	theme	CPO	530:532	arg1	supplementation					534:548	CPO supplementation	530:548	CPO supplementation	530:548	CPO supplementation decreased body weight and fat accumulation and improved glucose tolerance in HFD-fed mice.
35816111	8	43	theme	obesity-related	1386:1400	arg1	disorders					1412:1420	obesity-related metabolic disorders	1386:1420	obesity-related metabolic disorders	1386:1420	The results demonstrate that CPO supplementation can ameliorate HFD-induced obesity and obesity-related metabolic disorders.
35816111	0	44	from	profiles	94:101	arg1	mice					134:137	high-fat diet-induced obese mice	106:137	high-fat diet-induced obese mice	106:137	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	2	45	theme	related	360:366	arg1	complications					378:390	related metabolic complications	360:390	related metabolic complications	360:390	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	9	46	theme	microbiota	1453:1462	arg1	modulator					1464:1472	a novel gut microbiota modulator	1441:1472	a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis	1441:1509	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	9	46	theme	microbiota	1453:1462	arg1	It					1423:1424	It	1423:1424	It	1423:1424	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	1	47	theme	gut	203:205	arg1	imbalance					218:226	gut microbiota imbalance	203:226	gut microbiota imbalance	203:226	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	0	48	theme	gut	50:52	arg1	microbiota					54:63	the gut microbiota	46:63	the gut microbiota	46:63	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	6	49	dep	regulate	812:819	arg1	increasing					863:872	increasing	863:872	increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium	863:960	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	49	dep	regulate	812:819	arg1	decreasing					966:975	decreasing	966:975	decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice	966:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	5	50	theme	HFD	679:681	arg1	effects					664:670	the effects	660:670	the effects of the HFD on inflammatory markers and improved macrophage infiltration	660:742	CPOs also reversed the effects of the HFD on inflammatory markers and improved macrophage infiltration.
35816111	2	51	theme	induced	415:421	arg1	mice					429:432	high-fat diet (HFD) induced obese mice	395:432	high-fat diet (HFD) induced obese mice	395:432	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	2	52	dep	induced	415:421	arg1	HFD					410:412	HFD	410:412	HFD	410:412	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	2	52	dep	induced	415:421	arg1	diet					404:407	high-fat diet	395:407	high-fat diet (HFD)	395:413	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	3	53	theme	Male	435:438	arg1	mice					449:452	Male C57BL/6J mice	435:452	Male C57BL/6J mice	435:452	Male C57BL/6J mice were fed with a HFD for 16 weeks and treated daily with CPOs (500 mg kg-1).
35816111	1	54	theme	microbiota	207:216	arg1	imbalance					218:226	gut microbiota imbalance	203:226	gut microbiota imbalance	203:226	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	1	55	attach	linked	193:198	arg1	inflammation					240:251	chronic inflammation	232:251	chronic inflammation	232:251	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	1	55	attach	linked	193:198	arg2	problem					174:180	a major health problem worldwide	159:190	a major health problem worldwide	159:190	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	1	55	attach	linked	193:198	arg1	imbalance					218:226	gut microbiota imbalance	203:226	gut microbiota imbalance	203:226	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	5	56	theme	improved	711:718	arg1	infiltration					731:742	improved macrophage infiltration	711:742	improved macrophage infiltration	711:742	CPOs also reversed the effects of the HFD on inflammatory markers and improved macrophage infiltration.
35816111	5	57	theme	inflammatory	686:697	arg1	markers					699:705	inflammatory markers	686:705	inflammatory markers	686:705	CPOs also reversed the effects of the HFD on inflammatory markers and improved macrophage infiltration.
35816111	6	58	theme	microbiota	825:834	arg1	composition					836:846	gut microbiota composition	821:846	gut microbiota composition	821:846	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	7	59	theme	key	1146:1148	arg1	metabolites					1150:1160	20 key metabolites	1143:1160	20 key metabolites responding to CPO treatment	1143:1188	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	6	60	theme	gut	821:823	arg1	composition					836:846	gut microbiota composition	821:846	gut microbiota composition	821:846	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	7	61	theme	serum	1101:1105	arg1	analysis					1133:1140	serum non-targeted metabolomics analysis	1101:1140	serum non-targeted metabolomics analysis	1101:1140	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	2	62	from	complications	378:390	arg1	mice					429:432	high-fat diet (HFD) induced obese mice	395:432	high-fat diet (HFD) induced obese mice	395:432	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	6	63	dep	bacteria	906:913	arg1	Alistipes					936:944	Alistipes	936:944	Alistipes	936:944	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	63	dep	bacteria	906:913	arg1	bacteria					906:913	the beneficial bacteria	891:913	the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium	891:960	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	63	dep	bacteria	906:913	arg1	Clostridium					950:960	Clostridium	950:960	Clostridium	950:960	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	6	63	dep	bacteria	906:913	arg1	Muribaculaceae					915:928	Muribaculaceae	915:928	Muribaculaceae	915:928	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	4	64	theme	fat	576:578	arg1	accumulation					580:591	fat accumulation	576:591	fat accumulation	576:591	CPO supplementation decreased body weight and fat accumulation and improved glucose tolerance in HFD-fed mice.
35816111	7	65	theme	acid	1281:1284	arg1	metabolism					1286:1295	bile acid metabolism	1276:1295	bile acid metabolism	1276:1295	Based on serum non-targeted metabolomics analysis, 20 key metabolites responding to CPO treatment were identified, and their biological functions were mainly related to tryptophan and bile acid metabolism.
35816111	6	66	theme	analysis	775:782	arg1	results					749:755	The results	745:755	The results of gut microbiota analysis	745:782	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35816111	1	67	theme	chronic	232:238	arg1	inflammation					240:251	chronic inflammation	232:251	chronic inflammation	232:251	Obesity has become a major health problem worldwide, linked to gut microbiota imbalance and chronic inflammation.
35816111	0	68	theme	serum	76:80	arg1	profiles					94:101	serum metabolomic profiles	76:101	serum metabolomic profiles in high-fat diet-induced obese mice	76:137	Codonopsis pilosula oligosaccharides modulate the gut microbiota and change serum metabolomic profiles in high-fat diet-induced obese mice.
35816111	9	69	theme	gut	1497:1499	arg1	dysbiosis					1501:1509	HFD-induced gut dysbiosis	1485:1509	HFD-induced gut dysbiosis	1485:1509	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	2	70	theme	metabolic	368:376	arg1	complications					378:390	related metabolic complications	360:390	related metabolic complications	360:390	This study aims to evaluate whether Codonopsis pilosula oligosaccharides (CPOs) can alleviate obesity and related metabolic complications in high-fat diet (HFD) induced obese mice.
35816111	4	71	theme	HFD-fed	627:633	arg1	mice					635:638	HFD-fed mice	627:638	HFD-fed mice	627:638	CPO supplementation decreased body weight and fat accumulation and improved glucose tolerance in HFD-fed mice.
35816111	9	72	theme	gut	1449:1451	arg1	modulator					1464:1472	a novel gut microbiota modulator	1441:1472	a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis	1441:1509	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	9	72	theme	gut	1449:1451	arg1	It					1423:1424	It	1423:1424	It	1423:1424	It can be used as a novel gut microbiota modulator to prevent HFD-induced gut dysbiosis.
35816111	8	73	theme	CPO	1327:1329	arg1	supplementation					1331:1345	CPO supplementation	1327:1345	CPO supplementation	1327:1345	The results demonstrate that CPO supplementation can ameliorate HFD-induced obesity and obesity-related metabolic disorders.
35816111	6	74	theme	bacteria	1006:1013	arg1	abundance					981:989	the abundance	977:989	the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice	977:1089	The results of gut microbiota analysis showed that CPOs could also regulate gut microbiota composition, significantly increasing the abundance of the beneficial bacteria Muribaculaceae spp., Alistipes and Clostridium and decreasing the abundance of the harmful bacteria Rikenella, Enterobacteriaceae spp., Collinsella and Megasphaera in HFD mice.
35476407	7	0	theme	EC/PVP	1400:1405	arg1	K12					1407:1409	Both EC/PVP K12 and EC/PVP K25 blends	1395:1431	K12	1407:1409	Both EC/PVP K12 and EC/PVP K25 blends were miscible over the entire composition range, and addition of indomethacin did not alter this.
35476407	4	1	theme	molecular	769:777	arg1	weight					779:784	the molecular weight	765:784	the molecular weight of PVP	765:791	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	2	theme	drug	907:910	arg1	release					912:918	the drug release	903:918	the drug release from these solid dispersions	903:947	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	8	3	theme	EC	1726:1727	arg1	network					1729:1735	the EC network	1722:1735	the EC network	1722:1735	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	10	4	dep	work	1908:1911	arg1	the					1890:1892	the	1890:1892	the	1890:1892	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	10	4	dep	work	1908:1911	arg1	basis					1894:1898	basis	1894:1898	basis	1894:1898	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	0	5	theme	Agent	73:77	arg1	Potential					18:26	the Potential	14:26	the Potential of Ethyl Cellulose and a Porosity-Increasing Agent	14:77	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	0	5	theme	Agent	73:77	arg1	System					92:97	a Carrier System	82:97	a Carrier System for the Formulation of Amorphous Solid Dispersions	82:148	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	10	6	from	system	1980:1985	arg1	release					1946:1952	drug release	1941:1952	drug release from this complex ternary system	1941:1985	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	3	7	theme	initial	515:521	arg1	hypothesis					523:532	Our initial hypothesis	511:532	Our initial hypothesis	511:532	Our initial hypothesis was that upon contact with the dissolution medium, PVP would be released, creating a porous EC matrix through which the model drug indomethacin could diffuse.
35476407	8	8	theme	faster	1613:1618	arg1	release					1625:1631	faster drug release	1613:1631	faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created	1613:1766	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	3	9	theme	drug	660:663	arg1	indomethacin					665:676	the model drug indomethacin	650:676	the model drug indomethacin	650:676	Our initial hypothesis was that upon contact with the dissolution medium, PVP would be released, creating a porous EC matrix through which the model drug indomethacin could diffuse.
35476407	1	10	theme	amorphous	328:336	arg1	dispersions					344:354	amorphous solid dispersions	328:354	amorphous solid dispersions	328:354	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	2	11	theme	less	482:485	arg1	risk					487:490	less risk	482:490	less risk for precipitation	482:508	The rationale was that by conjoining these two different types of carriers a more gradual drug release could be created with less risk for precipitation.
35476407	9	12	from	view	1783:1786	arg1	mechanism					1803:1811	the release mechanism	1791:1811	the release mechanism	1791:1811	However, this view on the release mechanism appeared to be too simplistic as an optimum was observed for both blends.
35476407	0	13	theme	Amorphous	122:130	arg1	Dispersions					138:148	Amorphous Solid Dispersions	122:148	Amorphous Solid Dispersions	122:148	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	1	14	theme	dispersions	344:354	arg1	formulation					313:323	the formulation	309:323	the formulation of amorphous solid dispersions	309:354	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	9	15	theme	release	1795:1801	arg1	mechanism					1803:1811	the release mechanism	1791:1811	the release mechanism	1791:1811	However, this view on the release mechanism appeared to be too simplistic as an optimum was observed for both blends.
35476407	5	16	used	used	1087:1090	arg2	calorimetry					1026:1036	modulated differential scanning calorimetry	994:1036	modulated differential scanning calorimetry	994:1036	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	5	16	used	used	1087:1090	arg2	resonance					1072:1080	solid-state nuclear magnetic resonance	1043:1080	solid-state nuclear magnetic resonance	1043:1080	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	5	16	used	used	1087:1090	arg2	diffraction					981:991	X-ray powder diffraction	968:991	X-ray powder diffraction	968:991	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	0	17	theme	Dispersions	138:148	arg1	Formulation					107:117	the Formulation	103:117	the Formulation of Amorphous Solid Dispersions	103:148	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	5	18	theme	dispersions	1172:1182	arg1	properties					1135:1144	the miscibility and solid-state properties	1103:1144	the miscibility and solid-state properties of these blends and solid dispersions	1103:1182	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	6	19	theme	nuclear	1197:1203	arg1	resonance					1214:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	6	19	theme	nuclear	1197:1203	arg1	technique					1249:1257	a crucial technique	1239:1257	a crucial technique for this aspect	1239:1273	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	3	20	theme	dissolution	565:575	arg1	medium					577:582	the dissolution medium	561:582	the dissolution medium	561:582	Our initial hypothesis was that upon contact with the dissolution medium, PVP would be released, creating a porous EC matrix through which the model drug indomethacin could diffuse.
35476407	2	21	theme	gradual	439:445	arg1	release					452:458	a more gradual drug release	432:458	a more gradual drug release	432:458	The rationale was that by conjoining these two different types of carriers a more gradual drug release could be created with less risk for precipitation.
35476407	2	22	theme	drug	447:450	arg1	release					452:458	a more gradual drug release	432:458	a more gradual drug release	432:458	The rationale was that by conjoining these two different types of carriers a more gradual drug release could be created with less risk for precipitation.
35476407	6	23	theme	differential	1288:1299	arg1	calorimetry					1310:1320	modulated differential scanning calorimetry	1278:1320	modulated differential scanning calorimetry	1278:1320	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	4	24	from	observations	709:720	arg1	miscibility					825:835	the miscibility	821:835	the miscibility of the polymer blend	821:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	1	25	theme	carrier	290:296	arg1	polymer					185:191	an insoluble polymer	172:191	an insoluble polymer	172:191	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	1	25	theme	carrier	290:296	arg1	system					298:303	a carrier system	288:303	a carrier system for the formulation of amorphous solid dispersions	288:354	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	4	26	from	basis	700:704	arg1	miscibility					825:835	the miscibility	821:835	the miscibility of the polymer blend	821:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	0	27	theme	Carrier	84:90	arg1	Potential					18:26	the Potential	14:26	the Potential of Ethyl Cellulose and a Porosity-Increasing Agent	14:77	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	0	27	theme	Carrier	84:90	arg1	System					92:97	a Carrier System	82:97	a Carrier System for the Formulation of Amorphous Solid Dispersions	82:148	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	7	28	theme	entire	1456:1461	arg1	range					1475:1479	the entire composition range	1452:1479	the entire composition range	1452:1479	Both EC/PVP K12 and EC/PVP K25 blends were miscible over the entire composition range, and addition of indomethacin did not alter this.
35476407	8	29	with	release	1625:1631	arg1	probability					1647:1657	a higher probability	1638:1657	a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created	1638:1766	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	5	30	theme	powder	974:979	arg1	diffraction					981:991	X-ray powder diffraction	968:991	X-ray powder diffraction	968:991	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	4	31	theme	dispersion	888:897	arg1	state					869:873	the solid state	859:873	the solid state of the solid dispersion	859:897	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	31	theme	dispersion	888:897	arg1	release					912:918	the drug release	903:918	the drug release from these solid dispersions	903:947	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	32	theme	blend	852:856	arg1	miscibility					825:835	the miscibility	821:835	the miscibility of the polymer blend	821:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	5	33	theme	nuclear	1055:1061	arg1	resonance					1072:1080	solid-state nuclear magnetic resonance	1043:1080	solid-state nuclear magnetic resonance	1043:1080	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	10	34	theme	EC/PVP	2025:2030	arg1	ratio					2016:2020	the ratio	2012:2020	the ratio of EC/PVP	2012:2030	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	2	35	theme	carriers	423:430	arg1	types					414:418	these two different types	394:418	these two different types of carriers	394:430	The rationale was that by conjoining these two different types of carriers a more gradual drug release could be created with less risk for precipitation.
35476407	0	36	theme	Ethyl	31:35	arg1	Cellulose					37:45	Ethyl Cellulose	31:45	Ethyl Cellulose	31:45	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	0	37	theme	Porosity-Increasing	53:71	arg1	Agent					73:77	a Porosity-Increasing Agent	51:77	a Porosity-Increasing Agent	51:77	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	7	38	theme	EC/PVP	1415:1420	arg1	blends					1426:1431	Both EC/PVP K12 and EC/PVP K25 blends	1395:1431	blends	1426:1431	Both EC/PVP K12 and EC/PVP K25 blends were miscible over the entire composition range, and addition of indomethacin did not alter this.
35476407	4	39	theme	observations	709:720	arg1	basis					700:704	the basis	696:704	the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend	696:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	8	40	theme	drug	1546:1549	arg1	release					1551:1557	the drug release	1542:1557	the drug release	1542:1557	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	5	41	theme	miscibility	1107:1117	arg1	properties					1135:1144	the miscibility and solid-state properties	1103:1144	the miscibility and solid-state properties of these blends and solid dispersions	1103:1182	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	6	42	theme	scanning	1301:1308	arg1	calorimetry					1310:1320	modulated differential scanning calorimetry	1278:1320	modulated differential scanning calorimetry	1278:1320	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	4	43	theme	EC	725:726	arg1	effect					755:760	the effect	751:760	the effect of the molecular weight of PVP	751:791	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	43	theme	EC	725:726	arg1	observations					709:720	observations	709:720	observations of EC as a coating material	709:748	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	43	theme	EC	725:726	arg1	ratio					802:806	the ratio	798:806	the ratio of EC/PVP on the miscibility of the polymer blend	798:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	6	44	theme	systems	1386:1392	arg1	behavior					1368:1375	the complex phase behavior	1350:1375	the complex phase behavior of these systems	1350:1392	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	5	45	theme	solid-state	1123:1133	arg1	properties					1135:1144	the miscibility and solid-state properties	1103:1144	the miscibility and solid-state properties of these blends and solid dispersions	1103:1182	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	8	46	dep	probability	1647:1657	arg1	able					1699:1702	able	1699:1702	able	1699:1702	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	6	47	theme	Solid-state	1185:1195	arg1	resonance					1214:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	6	47	theme	Solid-state	1185:1195	arg1	technique					1249:1257	a crucial technique	1239:1257	a crucial technique for this aspect	1239:1273	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	10	48	theme	PVP	2060:2062	arg1	weight					2050:2055	the molecular weight	2036:2055	the molecular weight of PVP	2036:2062	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	3	49	theme	porous	619:624	arg1	matrix					629:634	a porous EC matrix	617:634	a porous EC matrix through which the model drug indomethacin could diffuse	617:690	Our initial hypothesis was that upon contact with the dissolution medium, PVP would be released, creating a porous EC matrix through which the model drug indomethacin could diffuse.
35476407	6	50	theme	phase	1362:1366	arg1	behavior					1368:1375	the complex phase behavior	1350:1375	the complex phase behavior of these systems	1350:1392	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	4	51	from	miscibility	825:835	arg1	basis					700:704	the basis	696:704	the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend	696:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	52	theme	weight	779:784	arg1	effect					755:760	the effect	751:760	the effect of the molecular weight of PVP	751:791	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	52	theme	weight	779:784	arg1	observations					709:720	observations	709:720	observations of EC as a coating material	709:748	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	52	theme	weight	779:784	arg1	ratio					802:806	the ratio	798:806	the ratio of EC/PVP on the miscibility of the polymer blend	798:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	3	53	theme	model	654:658	arg1	indomethacin					665:676	the model drug indomethacin	650:676	the model drug indomethacin	650:676	Our initial hypothesis was that upon contact with the dissolution medium, PVP would be released, creating a porous EC matrix through which the model drug indomethacin could diffuse.
35476407	8	54	theme	more	1740:1743	arg1	pores					1745:1749	more pores	1740:1749	more pores	1740:1749	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	1	55	theme	present	158:164	arg1	work					166:169	the present work	154:169	the present work	154:169	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	5	56	theme	differential	1004:1015	arg1	calorimetry					1026:1036	modulated differential scanning calorimetry	994:1036	modulated differential scanning calorimetry	994:1036	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	4	57	theme	effect	755:760	arg1	basis					700:704	the basis	696:704	the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend	696:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	10	58	theme	ternary	1972:1978	arg1	system					1980:1985	this complex ternary system	1959:1985	this complex ternary system	1959:1985	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	6	59	theme	crucial	1241:1247	arg1	resonance					1214:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	6	59	theme	crucial	1241:1247	arg1	technique					1249:1257	a crucial technique	1239:1257	a crucial technique for this aspect	1239:1273	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	1	60	theme	insoluble	175:183	arg1	polymer					185:191	an insoluble polymer	172:191	an insoluble polymer	172:191	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	1	60	theme	insoluble	175:183	arg1	system					298:303	a carrier system	288:303	a carrier system for the formulation of amorphous solid dispersions	288:354	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	4	61	theme	ratio	802:806	arg1	basis					700:704	the basis	696:704	the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend	696:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	1	62	dep	cellulose	206:214	arg1	i.e.					194:197	i.e.	194:197	i.e.	194:197	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	10	63	theme	drug	1941:1944	arg1	release					1946:1952	drug release	1941:1952	drug release from this complex ternary system	1941:1985	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	0	64	theme	Solid	132:136	arg1	Dispersions					138:148	Amorphous Solid Dispersions	122:148	Amorphous Solid Dispersions	122:148	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	1	65	theme	solid	338:342	arg1	dispersions					344:354	amorphous solid dispersions	328:354	amorphous solid dispersions	328:354	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	8	66	theme	drug	1620:1623	arg1	release					1625:1631	faster drug release	1613:1631	faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created	1613:1766	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	4	67	theme	EC/PVP	811:816	arg1	effect					755:760	the effect	751:760	the effect of the molecular weight of PVP	751:791	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	67	theme	EC/PVP	811:816	arg1	observations					709:720	observations	709:720	observations of EC as a coating material	709:748	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	67	theme	EC/PVP	811:816	arg1	ratio					802:806	the ratio	798:806	the ratio of EC/PVP on the miscibility of the polymer blend	798:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	68	from	ratio	802:806	arg1	miscibility					825:835	the miscibility	821:835	the miscibility of the polymer blend	821:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	7	69	theme	indomethacin	1498:1509	arg1	addition					1486:1493	addition	1486:1493	addition of indomethacin	1486:1509	Both EC/PVP K12 and EC/PVP K25 blends were miscible over the entire composition range, and addition of indomethacin did not alter this.
35476407	4	70	theme	PVP	789:791	arg1	weight					779:784	the molecular weight	765:784	the molecular weight of PVP	765:791	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	10	71	theme	complex	1964:1970	arg1	system					1980:1985	this complex ternary system	1959:1985	this complex ternary system	1959:1985	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	5	72	theme	solid	1166:1170	arg1	dispersions					1172:1182	solid dispersions	1166:1182	solid dispersions	1166:1182	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	6	73	theme	magnetic	1205:1212	arg1	resonance					1214:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance	1185:1222	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	6	73	theme	magnetic	1205:1212	arg1	technique					1249:1257	a crucial technique	1239:1257	a crucial technique for this aspect	1239:1273	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	8	74	theme	higher	1640:1645	arg1	probability					1647:1657	a higher probability	1638:1657	a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created	1638:1766	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	4	75	from	dispersions	937:947	arg1	state					869:873	the solid state	859:873	the solid state of the solid dispersion	859:897	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	75	from	dispersions	937:947	arg1	release					912:918	the drug release	903:918	the drug release from these solid dispersions	903:947	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	3	76	with	contact	548:554	arg1	medium					577:582	the dissolution medium	561:582	the dissolution medium	561:582	Our initial hypothesis was that upon contact with the dissolution medium, PVP would be released, creating a porous EC matrix through which the model drug indomethacin could diffuse.
35476407	4	77	theme	polymer	844:850	arg1	blend					852:856	the polymer blend	840:856	the polymer blend	840:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	6	78	theme	modulated	1278:1286	arg1	calorimetry					1310:1320	modulated differential scanning calorimetry	1278:1320	modulated differential scanning calorimetry	1278:1320	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	5	79	theme	modulated	994:1002	arg1	calorimetry					1026:1036	modulated differential scanning calorimetry	994:1036	modulated differential scanning calorimetry	994:1036	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	7	80	theme	composition	1463:1473	arg1	range					1475:1479	the entire composition range	1452:1479	the entire composition range	1452:1479	Both EC/PVP K12 and EC/PVP K25 blends were miscible over the entire composition range, and addition of indomethacin did not alter this.
35476407	10	81	theme	formulations	2142:2153	arg1	wettability					2123:2133	the wettability	2119:2133	the wettability of the formulations	2119:2153	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	10	81	theme	formulations	2142:2153	arg1	interactions					2076:2087	interactions	2076:2087	interactions between the three components	2076:2116	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	10	81	theme	formulations	2142:2153	arg1	layer					2174:2178	the viscosity layer	2160:2178	the viscosity layer that was created around the particles	2160:2216	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	5	82	theme	blends	1155:1160	arg1	properties					1135:1144	the miscibility and solid-state properties	1103:1144	the miscibility and solid-state properties of these blends and solid dispersions	1103:1182	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	5	83	theme	scanning	1017:1024	arg1	calorimetry					1026:1036	modulated differential scanning calorimetry	994:1036	modulated differential scanning calorimetry	994:1036	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	8	84	theme	drug	1675:1678	arg1	molecules					1680:1688	the drug molecules	1671:1688	the drug molecules	1671:1688	Concerning the drug release, it was initially thought that more PVP would lead to faster drug release with a higher probability that all of the drug molecules would be able to diffuse out of the EC network as more pores would be created.
35476407	5	85	theme	X-ray	968:972	arg1	diffraction					981:991	X-ray powder diffraction	968:991	X-ray powder diffraction	968:991	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	10	86	theme	viscosity	2164:2172	arg1	layer					2174:2178	the viscosity layer	2160:2178	the viscosity layer that was created around the particles	2160:2216	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	10	87	theme	molecular	2040:2048	arg1	weight					2050:2055	the molecular weight	2036:2055	the molecular weight of PVP	2036:2062	On the basis of this work, it could be concluded that drug release from this complex ternary system was affected not only by the ratio of EC/PVP and the molecular weight of PVP but also by interactions between the three components, the wettability of the formulations, and the viscosity layer that was created around the particles.
35476407	6	88	theme	complex	1354:1360	arg1	behavior					1368:1375	the complex phase behavior	1350:1375	the complex phase behavior of these systems	1350:1392	Solid-state nuclear magnetic resonance appeared to be a crucial technique for this aspect as modulated differential scanning calorimetry was not sufficient to grasp the complex phase behavior of these systems.
35476407	4	89	from	effect	755:760	arg1	miscibility					825:835	the miscibility	821:835	the miscibility of the polymer blend	821:856	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	4	90	theme	solid	882:886	arg1	dispersion					888:897	the solid dispersion	878:897	the solid dispersion	878:897	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	5	91	theme	magnetic	1063:1070	arg1	resonance					1072:1080	solid-state nuclear magnetic resonance	1043:1080	solid-state nuclear magnetic resonance	1043:1080	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	0	92	theme	Cellulose	37:45	arg1	Potential					18:26	the Potential	14:26	the Potential of Ethyl Cellulose and a Porosity-Increasing Agent	14:77	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	0	92	theme	Cellulose	37:45	arg1	System					92:97	a Carrier System	82:97	a Carrier System for the Formulation of Amorphous Solid Dispersions	82:148	Investigating the Potential of Ethyl Cellulose and a Porosity-Increasing Agent as a Carrier System for the Formulation of Amorphous Solid Dispersions.
35476407	1	93	theme	water-soluble	244:256	arg1	PVP					280:282	PVP	280:282	PVP	280:282	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	1	93	theme	water-soluble	244:256	arg1	polyvinylpyrrolidone					258:277	the water-soluble polyvinylpyrrolidone	240:277	the water-soluble polyvinylpyrrolidone (PVP)	240:283	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	4	94	theme	solid	863:867	arg1	state					869:873	the solid state	859:873	the solid state of the solid dispersion	859:897	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	2	95	theme	different	404:412	arg1	types					414:418	these two different types	394:418	these two different types of carriers	394:430	The rationale was that by conjoining these two different types of carriers a more gradual drug release could be created with less risk for precipitation.
35476407	4	96	theme	coating	733:739	arg1	material					741:748	a coating material	731:748	a coating material	731:748	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	5	97	theme	solid-state	1043:1053	arg1	resonance					1072:1080	solid-state nuclear magnetic resonance	1043:1080	solid-state nuclear magnetic resonance	1043:1080	X-ray powder diffraction, modulated differential scanning calorimetry, and solid-state nuclear magnetic resonance were used to unravel the miscibility and solid-state properties of these blends and solid dispersions.
35476407	7	98	theme	K25	1422:1424	arg1	blends					1426:1431	Both EC/PVP K12 and EC/PVP K25 blends	1395:1431	blends	1426:1431	Both EC/PVP K12 and EC/PVP K25 blends were miscible over the entire composition range, and addition of indomethacin did not alter this.
35476407	4	99	theme	solid	931:935	arg1	dispersions					937:947	these solid dispersions	925:947	these solid dispersions	925:947	On the basis of observations of EC as a coating material, the effect of the molecular weight of PVP, and the ratio of EC/PVP on the miscibility of the polymer blend, the solid state of the solid dispersion and the drug release from these solid dispersions were investigated.
35476407	1	100	theme	ethyl	200:204	arg1	cellulose					206:214	ethyl cellulose	200:214	ethyl cellulose (EC)	200:219	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	1	100	theme	ethyl	200:204	arg1	EC					217:218	EC	217:218	EC	217:218	In the present work, an insoluble polymer, i.e., ethyl cellulose (EC), was combined with the water-soluble polyvinylpyrrolidone (PVP) as a carrier system for the formulation of amorphous solid dispersions.
35476407	3	101	theme	EC	626:627	arg1	matrix					629:634	a porous EC matrix	617:634	a porous EC matrix through which the model drug indomethacin could diffuse	617:690	Our initial hypothesis was that upon contact with the dissolution medium, PVP would be released, creating a porous EC matrix through which the model drug indomethacin could diffuse.
35526287	1	0	theme	concentrate	320:330	arg1	WPC					333:335	whey protein concentrate (WPC)	307:336	whey protein concentrate (WPC)	307:336	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	3	1	theme	Rice	725:728	arg1	flour					730:734	Rice flour	725:734	Rice flour (100%)	725:741	Rice flour (100%) was used as a control.
35526287	3	1	theme	Rice	725:728	arg1	%					740:740	100%	737:740	100%	737:740	Rice flour (100%) was used as a control.
35526287	3	1	theme	Rice	725:728	arg1	control					757:763	a control	755:763	a control	755:763	Rice flour (100%) was used as a control.
35526287	7	2	theme	antioxidant	1191:1201	arg1	properties					1203:1212	the TPC and antioxidant properties	1179:1212	the TPC and antioxidant properties of extruded snacks	1179:1231	Extrusion significantly reduced the TPC and antioxidant properties of extruded snacks compared to their raw counterparts.
35526287	8	3	theme	healthy	1389:1395	arg1	snacks					1424:1429	healthy novel gluten-free expanded snacks	1389:1429	healthy novel gluten-free expanded snacks with bioactive compounds	1389:1454	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	4	4	theme	fibre	873:877	arg1	content					879:885	the insoluble fibre content	859:885	the insoluble fibre content	859:885	Extrusion increased the oligosaccharides (2-3 fold) and resistant starch (1-3 fold), whereas the insoluble fibre content was not significantly affected.
35526287	0	5	theme	snacks	167:172	arg1	composition					98:108	amino acid composition	87:108	amino acid composition	87:108	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	5	theme	snacks	167:172	arg1	Effects					0:6	Effects	0:6	Effects of extrusion processing on the bioactive constituents	0:60	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	5	theme	snacks	167:172	arg1	digestibility					72:84	in vitro digestibility	63:84	in vitro digestibility	63:84	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	5	theme	snacks	167:172	arg1	potential					127:135	antioxidant potential	115:135	antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein	115:211	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	6	6	from	increase	1080:1087	arg1	content					1107:1113	total phenolic content	1092:1113	total phenolic content (TPC)	1092:1119	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	6	6	from	increase	1080:1087	arg1	activity					1137:1144	antioxidant activity	1125:1144	antioxidant activity	1125:1144	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	6	6	from	increase	1080:1087	arg1	TPC					1116:1118	TPC	1116:1118	TPC	1116:1118	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	8	7	with	snacks	1424:1429	arg1	compounds					1446:1454	bioactive compounds	1436:1454	bioactive compounds	1436:1454	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	6	8	contain	had	1073:1075	arg2	increase					1080:1087	an increase	1077:1087	an increase in total phenolic content (TPC) and antioxidant activity	1077:1144	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	6	8	contain	had	1073:1075	arg1	samples					1036:1042	Extruded and raw samples	1019:1042	samples	1036:1042	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	0	9	theme	amino	87:91	arg1	composition					98:108	amino acid composition	87:108	amino acid composition	87:108	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	10	from	Effects	0:6	arg1	constituents					49:60	the bioactive constituents	35:60	the bioactive constituents	35:60	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	2	11	theme	amino	585:589	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	11	theme	amino	585:589	arg1	composition					596:606	amino acid composition	585:606	amino acid composition	585:606	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	4	12	theme	insoluble	863:871	arg1	content					879:885	the insoluble fibre content	859:885	the insoluble fibre content	859:885	Extrusion increased the oligosaccharides (2-3 fold) and resistant starch (1-3 fold), whereas the insoluble fibre content was not significantly affected.
35526287	6	13	theme	antioxidant	1125:1135	arg1	activity					1137:1144	antioxidant activity	1125:1144	antioxidant activity	1125:1144	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	1	14	theme	novel	254:258	arg1	snacks					272:277	novel gluten-free snacks	254:277	novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties	254:387	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	2	15	theme	phytic	519:524	arg1	oligosaccharides					458:473	sucrose-galactosyl oligosaccharides	439:473	sucrose-galactosyl oligosaccharides	439:473	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	15	theme	phytic	519:524	arg1	acid					526:529	phytic acid	519:529	phytic acid	519:529	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	1	16	from	cowpea	296:301	arg1	snacks					272:277	novel gluten-free snacks	254:277	novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties	254:387	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	2	17	theme	antioxidant	636:646	arg1	properties					648:657	antioxidant properties	636:657	antioxidant properties	636:657	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	17	theme	antioxidant	636:646	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	5	18	theme	in	939:940	arg1	p < 0.05					971:978	p < 0.05	971:978	p < 0.05	971:978	Extrusion increased in vitro protein digestibility (p < 0.05) and amino acid composition in snacks.
35526287	5	18	theme	in	939:940	arg1	digestibility					956:968	in vitro protein digestibility	939:968	in vitro protein digestibility (p < 0.05)	939:979	Extrusion increased in vitro protein digestibility (p < 0.05) and amino acid composition in snacks.
35526287	1	19	theme	gluten-free	260:270	arg1	snacks					272:277	novel gluten-free snacks	254:277	novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties	254:387	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	0	20	theme	whey	200:203	arg1	protein					205:211	whey protein	200:211	whey protein	200:211	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	21	theme	acid	93:96	arg1	composition					98:108	amino acid composition	87:108	amino acid composition	87:108	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	1	22	from	flour	289:293	arg1	snacks					272:277	novel gluten-free snacks	254:277	novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties	254:387	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	2	23	theme	raw	687:689	arg1	formulations					691:702	raw formulations	687:702	raw formulations	687:702	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	5	24	theme	protein	948:954	arg1	p < 0.05					971:978	p < 0.05	971:978	p < 0.05	971:978	Extrusion increased in vitro protein digestibility (p < 0.05) and amino acid composition in snacks.
35526287	5	24	theme	protein	948:954	arg1	digestibility					956:968	in vitro protein digestibility	939:968	in vitro protein digestibility (p < 0.05)	939:979	Extrusion increased in vitro protein digestibility (p < 0.05) and amino acid composition in snacks.
35526287	4	25	dep	increased	776:784	arg1	fold					844:847	1-3 fold	840:847	1-3 fold	840:847	Extrusion increased the oligosaccharides (2-3 fold) and resistant starch (1-3 fold), whereas the insoluble fibre content was not significantly affected.
35526287	4	25	dep	increased	776:784	arg1	whereas					851:857	whereas	851:857	whereas	851:857	Extrusion increased the oligosaccharides (2-3 fold) and resistant starch (1-3 fold), whereas the insoluble fibre content was not significantly affected.
35526287	1	26	theme	bioactive	352:360	arg1	properties					378:387	bioactive and antioxidant properties	352:387	bioactive and antioxidant properties	352:387	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	2	27	theme	total	609:613	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	27	theme	total	609:613	arg1	content					624:630	total phenolic content	609:630	total phenolic content	609:630	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	28	theme	resistant	501:509	arg1	oligosaccharides					458:473	sucrose-galactosyl oligosaccharides	439:473	sucrose-galactosyl oligosaccharides	439:473	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	28	theme	resistant	501:509	arg1	starch					511:516	resistant starch	501:516	resistant starch	501:516	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	6	29	theme	raw	1032:1034	arg1	samples					1036:1042	Extruded and raw samples	1019:1042	samples	1036:1042	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	2	30	dep	compounds	428:436	arg1	oligosaccharides					458:473	sucrose-galactosyl oligosaccharides	439:473	sucrose-galactosyl oligosaccharides	439:473	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	30	dep	compounds	428:436	arg1	activity					551:558	trypsin inhibitory activity	532:558	trypsin inhibitory activity	532:558	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	30	dep	compounds	428:436	arg1	fibre					494:498	fibre	494:498	fibre	494:498	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	30	dep	compounds	428:436	arg1	starch					511:516	resistant starch	501:516	resistant starch	501:516	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	30	dep	compounds	428:436	arg1	acid					526:529	phytic acid	519:529	phytic acid	519:529	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	8	31	theme	growing	1319:1325	arg1	interest					1327:1334	the growing interest	1315:1334	the growing interest of the food industry to cater	1315:1364	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	2	32	theme	acid	591:594	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	32	theme	acid	591:594	arg1	composition					596:606	amino acid composition	585:606	amino acid composition	585:606	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	0	33	theme	processing	21:30	arg1	composition					98:108	amino acid composition	87:108	amino acid composition	87:108	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	33	theme	processing	21:30	arg1	Effects					0:6	Effects	0:6	Effects of extrusion processing on the bioactive constituents	0:60	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	33	theme	processing	21:30	arg1	digestibility					72:84	in vitro digestibility	63:84	in vitro digestibility	63:84	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	33	theme	processing	21:30	arg1	potential					127:135	antioxidant potential	115:135	antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein	115:211	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	4	34	theme	2-3	808:810	arg1	oligosaccharides					790:805	the oligosaccharides	786:805	the oligosaccharides (2-3 fold)	786:816	Extrusion increased the oligosaccharides (2-3 fold) and resistant starch (1-3 fold), whereas the insoluble fibre content was not significantly affected.
35526287	4	34	theme	2-3	808:810	arg1	fold					812:815	2-3 fold	808:815	2-3 fold	808:815	Extrusion increased the oligosaccharides (2-3 fold) and resistant starch (1-3 fold), whereas the insoluble fibre content was not significantly affected.
35526287	0	35	dep	in	63:64	arg1	vitro					66:70	vitro	66:70	vitro	66:70	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	2	36	theme	extrusion	405:413	arg1	effects					394:400	The effects	390:400	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties	390:657	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	0	37	theme	extrusion	11:19	arg1	processing					21:30	extrusion processing	11:30	extrusion processing	11:30	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	1	38	theme	rice	284:287	arg1	flour					289:293	rice flour	284:293	rice flour	284:293	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	2	39	theme	inhibitory	540:549	arg1	oligosaccharides					458:473	sucrose-galactosyl oligosaccharides	439:473	sucrose-galactosyl oligosaccharides	439:473	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	39	theme	inhibitory	540:549	arg1	activity					551:558	trypsin inhibitory activity	532:558	trypsin inhibitory activity	532:558	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	0	40	from	digestibility	72:84	arg1	constituents					49:60	the bioactive constituents	35:60	the bioactive constituents	35:60	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	6	41	theme	total	1092:1096	arg1	content					1107:1113	total phenolic content	1092:1113	total phenolic content (TPC)	1092:1119	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	6	41	theme	total	1092:1096	arg1	TPC					1116:1118	TPC	1116:1118	TPC	1116:1118	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	2	42	theme	trypsin	532:538	arg1	oligosaccharides					458:473	sucrose-galactosyl oligosaccharides	439:473	sucrose-galactosyl oligosaccharides	439:473	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	42	theme	trypsin	532:538	arg1	activity					551:558	trypsin inhibitory activity	532:558	trypsin inhibitory activity	532:558	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	1	43	theme	antioxidant	366:376	arg1	properties					378:387	bioactive and antioxidant properties	352:387	bioactive and antioxidant properties	352:387	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	8	44	theme	industry	1348:1355	arg1	interest					1327:1334	the growing interest	1315:1334	the growing interest of the food industry to cater	1315:1364	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	7	45	theme	raw	1251:1253	arg1	counterparts					1255:1266	their raw counterparts	1245:1266	their raw counterparts	1245:1266	Extrusion significantly reduced the TPC and antioxidant properties of extruded snacks compared to their raw counterparts.
35526287	1	46	from	WPC	333:335	arg1	snacks					272:277	novel gluten-free snacks	254:277	novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties	254:387	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	0	47	theme	antioxidant	115:125	arg1	potential					127:135	antioxidant potential	115:135	antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein	115:211	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	8	48	theme	expanded	1415:1422	arg1	snacks					1424:1429	healthy novel gluten-free expanded snacks	1389:1429	healthy novel gluten-free expanded snacks with bioactive compounds	1389:1454	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	2	49	theme	bioactive	418:426	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	49	theme	bioactive	418:426	arg1	properties					648:657	antioxidant properties	636:657	antioxidant properties	636:657	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	49	theme	bioactive	418:426	arg1	composition					596:606	amino acid composition	585:606	amino acid composition	585:606	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	49	theme	bioactive	418:426	arg1	digestibility					570:582	protein digestibility	562:582	protein digestibility	562:582	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	49	theme	bioactive	418:426	arg1	content					624:630	total phenolic content	609:630	total phenolic content	609:630	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	8	50	theme	novel	1397:1401	arg1	snacks					1424:1429	healthy novel gluten-free expanded snacks	1389:1429	healthy novel gluten-free expanded snacks with bioactive compounds	1389:1454	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	0	51	theme	novel	140:144	arg1	snacks					167:172	novel gluten-free extruded snacks	140:172	novel gluten-free extruded snacks fortified with cowpea and whey protein	140:211	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	8	52	theme	gluten-free	1403:1413	arg1	snacks					1424:1429	healthy novel gluten-free expanded snacks	1389:1429	healthy novel gluten-free expanded snacks with bioactive compounds	1389:1454	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	5	53	theme	acid	991:994	arg1	composition					996:1006	amino acid composition	985:1006	amino acid composition	985:1006	Extrusion increased in vitro protein digestibility (p < 0.05) and amino acid composition in snacks.
35526287	2	54	theme	extruded	708:715	arg1	snacks					717:722	extruded snacks	708:722	extruded snacks	708:722	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	0	55	theme	bioactive	39:47	arg1	constituents					49:60	the bioactive constituents	35:60	the bioactive constituents	35:60	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	2	56	theme	protein	562:568	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	56	theme	protein	562:568	arg1	digestibility					570:582	protein digestibility	562:582	protein digestibility	562:582	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	0	57	from	composition	98:108	arg1	constituents					49:60	the bioactive constituents	35:60	the bioactive constituents	35:60	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	2	58	theme	phenolic	615:622	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	58	theme	phenolic	615:622	arg1	content					624:630	total phenolic content	609:630	total phenolic content	609:630	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	7	59	theme	TPC	1183:1185	arg1	properties					1203:1212	the TPC and antioxidant properties	1179:1212	the TPC and antioxidant properties of extruded snacks	1179:1231	Extrusion significantly reduced the TPC and antioxidant properties of extruded snacks compared to their raw counterparts.
35526287	1	60	theme	whey	307:310	arg1	WPC					333:335	whey protein concentrate (WPC)	307:336	whey protein concentrate (WPC)	307:336	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	0	61	theme	extruded	158:165	arg1	snacks					167:172	novel gluten-free extruded snacks	140:172	novel gluten-free extruded snacks fortified with cowpea and whey protein	140:211	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	3	62	used	used	747:750	arg2	control					757:763	a control	755:763	a control	755:763	Rice flour (100%) was used as a control.
35526287	3	62	used	used	747:750	arg2	flour					730:734	Rice flour	725:734	Rice flour (100%)	725:741	Rice flour (100%) was used as a control.
35526287	3	62	used	used	747:750	arg2	%					740:740	100%	737:740	100%	737:740	Rice flour (100%) was used as a control.
35526287	8	63	theme	consumer	1369:1376	arg1	demand					1378:1383	consumer demand	1369:1383	consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds	1369:1454	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	5	64	theme	amino	985:989	arg1	composition					996:1006	amino acid composition	985:1006	amino acid composition	985:1006	Extrusion increased in vitro protein digestibility (p < 0.05) and amino acid composition in snacks.
35526287	7	65	theme	snacks	1226:1231	arg1	properties					1203:1212	the TPC and antioxidant properties	1179:1212	the TPC and antioxidant properties of extruded snacks	1179:1231	Extrusion significantly reduced the TPC and antioxidant properties of extruded snacks compared to their raw counterparts.
35526287	6	66	theme	phenolic	1098:1105	arg1	content					1107:1113	total phenolic content	1092:1113	total phenolic content (TPC)	1092:1119	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	6	66	theme	phenolic	1098:1105	arg1	TPC					1116:1118	TPC	1116:1118	TPC	1116:1118	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	0	67	from	potential	127:135	arg1	constituents					49:60	the bioactive constituents	35:60	the bioactive constituents	35:60	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	0	68	theme	gluten-free	146:156	arg1	snacks					167:172	novel gluten-free extruded snacks	140:172	novel gluten-free extruded snacks fortified with cowpea and whey protein	140:211	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	8	69	theme	food	1343:1346	arg1	industry					1348:1355	the food industry	1339:1355	the food industry	1339:1355	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	2	70	from	effects	394:400	arg1	compounds					428:436	bioactive compounds	418:436	bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity)	418:559	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	70	from	effects	394:400	arg1	properties					648:657	antioxidant properties	636:657	antioxidant properties	636:657	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	70	from	effects	394:400	arg1	composition					596:606	amino acid composition	585:606	amino acid composition	585:606	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	70	from	effects	394:400	arg1	digestibility					570:582	protein digestibility	562:582	protein digestibility	562:582	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	70	from	effects	394:400	arg1	content					624:630	total phenolic content	609:630	total phenolic content	609:630	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	5	71	dep	in	939:940	arg1	vitro					942:946	vitro	942:946	vitro	942:946	Extrusion increased in vitro protein digestibility (p < 0.05) and amino acid composition in snacks.
35526287	2	72	theme	sucrose-galactosyl	439:456	arg1	oligosaccharides					458:473	sucrose-galactosyl oligosaccharides	439:473	sucrose-galactosyl oligosaccharides	439:473	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	72	theme	sucrose-galactosyl	439:456	arg1	activity					551:558	trypsin inhibitory activity	532:558	trypsin inhibitory activity	532:558	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	72	theme	sucrose-galactosyl	439:456	arg1	fibre					494:498	fibre	494:498	fibre	494:498	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	72	theme	sucrose-galactosyl	439:456	arg1	starch					511:516	resistant starch	501:516	resistant starch	501:516	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	2	72	theme	sucrose-galactosyl	439:456	arg1	acid					526:529	phytic acid	519:529	phytic acid	519:529	The effects of extrusion on bioactive compounds (sucrose-galactosyl oligosaccharides, insoluble dietary fibre, resistant starch, phytic acid, trypsin inhibitory activity), protein digestibility, amino acid composition, total phenolic content and antioxidant properties were evaluated by comparing raw formulations and extruded snacks.
35526287	0	73	theme	in	63:64	arg1	digestibility					72:84	in vitro digestibility	63:84	in vitro digestibility	63:84	Effects of extrusion processing on the bioactive constituents, in vitro digestibility, amino acid composition, and antioxidant potential of novel gluten-free extruded snacks fortified with cowpea and whey protein concentrate.
35526287	8	74	theme	bioactive	1436:1444	arg1	compounds					1446:1454	bioactive compounds	1436:1454	bioactive compounds	1436:1454	The results obtained in this study respond to the growing interest of the food industry to cater to consumer demand for healthy novel gluten-free expanded snacks with bioactive compounds.
35526287	6	75	theme	Extruded	1019:1026	arg1	samples					1036:1042	Extruded and raw samples	1019:1042	samples	1036:1042	Extruded and raw samples enriched with cowpea and WPC had an increase in total phenolic content (TPC) and antioxidant activity.
35526287	1	76	theme	protein	312:318	arg1	WPC					333:335	whey protein concentrate (WPC)	307:336	whey protein concentrate (WPC)	307:336	This study aimed to develop novel gluten-free snacks from rice flour, cowpea and whey protein concentrate (WPC) enriched with bioactive and antioxidant properties.
35526287	4	77	theme	resistant	822:830	arg1	starch					832:837	resistant starch	822:837	resistant starch	822:837	Extrusion increased the oligosaccharides (2-3 fold) and resistant starch (1-3 fold), whereas the insoluble fibre content was not significantly affected.
35526287	7	78	theme	extruded	1217:1224	arg1	snacks					1226:1231	extruded snacks	1217:1231	extruded snacks	1217:1231	Extrusion significantly reduced the TPC and antioxidant properties of extruded snacks compared to their raw counterparts.
36276535	0	0	theme	polysaccharide	96:109	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	0	0	theme	polysaccharide	96:109	arg1	analysis					42:49	structural analysis	31:49	structural analysis	31:49	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	0	0	theme	polysaccharide	96:109	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	0	0	theme	polysaccharide	96:109	arg1	activities					67:76	antioxidant activities	55:76	antioxidant activities	55:76	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	1	1	from	effects	182:188	arg1	morphology					248:257	morphology	248:257	morphology	248:257	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	1	from	effects	182:188	arg1	activities					282:291	radical-scavenging activities	263:291	radical-scavenging activities	263:291	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	1	from	effects	182:188	arg1	attributes					236:245	structural attributes	225:245	structural attributes	225:245	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	1	from	effects	182:188	arg1	composition					212:222	the monosaccharide composition	193:222	the monosaccharide composition	193:222	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	2	theme	radical-scavenging	263:280	arg1	activities					282:291	radical-scavenging activities	263:291	radical-scavenging activities	263:291	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	0	3	theme	phosphorylated	81:94	arg1	polysaccharide					96:109	phosphorylated polysaccharide	81:109	phosphorylated polysaccharide	81:109	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	6	4	theme	increasing	969:978	arg1	tendencies					980:989	concentration-dependent increasing tendencies	945:989	concentration-dependent increasing tendencies in radical-scavenging activities	945:1022	Phosphorylation of polysaccharides led to concentration-dependent increasing tendencies in radical-scavenging activities.
36276535	0	5	theme	Sanchi	116:121	arg1	flower					143:148	Sanchi (Panax notoginseng) flower	116:148	Sanchi (Panax notoginseng) flower	116:148	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	6	6	theme	concentration-dependent	945:967	arg1	tendencies					980:989	concentration-dependent increasing tendencies	945:989	concentration-dependent increasing tendencies in radical-scavenging activities	945:1022	Phosphorylation of polysaccharides led to concentration-dependent increasing tendencies in radical-scavenging activities.
36276535	7	7	theme	positive	1057:1064	arg1	impact					1066:1071	the positive impact	1053:1071	the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent	1053:1179	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	4	8	theme	Sanchi	735:740	arg1	polysaccharides					749:763	the Sanchi flower polysaccharides	731:763	the Sanchi flower polysaccharides	731:763	NMR and FTIR analyses were indicative of the successful phosphorylation of the Sanchi flower polysaccharides.
36276535	3	9	theme	FTIR	528:531	arg1	analysis					533:540	FTIR analysis	528:540	FTIR analysis of phosphorylated polysaccharides	528:574	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36276535	1	10	theme	Sanchi	296:301	arg1	polysaccharides					330:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	0	11	theme	Panax	124:128	arg1	flower					143:148	Sanchi (Panax notoginseng) flower	116:148	Sanchi (Panax notoginseng) flower	116:148	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	5	12	from	induction	835:843	arg1	configuration					888:900	polysaccharides configuration	872:900	polysaccharides configuration	872:900	The introduction of phosphate groups into polysaccharides led to the induction of pore-like structures in polysaccharides configuration.
36276535	1	13	theme	phosphorylation	166:180	arg1	effects					182:188	phosphorylation effects	166:188	phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides	166:344	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	6	14	theme	polysaccharides	922:936	arg1	Phosphorylation					903:917	Phosphorylation	903:917	Phosphorylation of polysaccharides	903:936	Phosphorylation of polysaccharides led to concentration-dependent increasing tendencies in radical-scavenging activities.
36276535	7	15	theme	therapeutic	1163:1173	arg1	agent					1175:1179	a therapeutic agent	1161:1179	a therapeutic agent	1161:1179	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	7	15	theme	therapeutic	1163:1173	arg1	polysaccharides					1109:1123	Sanchi flower polysaccharides	1095:1123	Sanchi flower polysaccharides	1095:1123	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	6	16	from	tendencies	980:989	arg1	activities					1013:1022	radical-scavenging activities	994:1022	radical-scavenging activities	994:1022	Phosphorylation of polysaccharides led to concentration-dependent increasing tendencies in radical-scavenging activities.
36276535	3	17	theme	phosphorylated	545:558	arg1	polysaccharides					560:574	phosphorylated polysaccharides	545:574	phosphorylated polysaccharides	545:574	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36276535	4	18	theme	FTIR	664:667	arg1	analyses					669:676	FTIR analyses	664:676	FTIR analyses	664:676	NMR and FTIR analyses were indicative of the successful phosphorylation of the Sanchi flower polysaccharides.
36276535	7	19	used	used	1153:1156	arg2	agent					1175:1179	a therapeutic agent	1161:1179	a therapeutic agent	1161:1179	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	7	19	used	used	1153:1156	arg2	polysaccharides					1109:1123	Sanchi flower polysaccharides	1095:1123	Sanchi flower polysaccharides	1095:1123	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	5	20	theme	polysaccharides	872:886	arg1	configuration					888:900	polysaccharides configuration	872:900	polysaccharides configuration	872:900	The introduction of phosphate groups into polysaccharides led to the induction of pore-like structures in polysaccharides configuration.
36276535	3	21	theme	polysaccharides	560:574	arg1	analysis					533:540	FTIR analysis	528:540	FTIR analysis of phosphorylated polysaccharides	528:574	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36276535	3	22	theme	1254 cm-1	645:653	arg1	region					635:640	spectral region	626:640	spectral region of 1254 cm-1	626:653	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36276535	1	23	theme	Panax	304:308	arg1	polysaccharides					330:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	4	24	theme	polysaccharides	749:763	arg1	phosphorylation					712:726	the successful phosphorylation	697:726	the successful phosphorylation of the Sanchi flower polysaccharides	697:763	NMR and FTIR analyses were indicative of the successful phosphorylation of the Sanchi flower polysaccharides.
36276535	1	25	theme	monosaccharide	197:210	arg1	composition					212:222	the monosaccharide composition	193:222	the monosaccharide composition	193:222	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	26	theme	notoginseng	310:320	arg1	polysaccharides					330:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	0	27	theme	notoginseng	130:140	arg1	flower					143:148	Sanchi (Panax notoginseng) flower	116:148	Sanchi (Panax notoginseng) flower	116:148	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	4	28	theme	flower	742:747	arg1	polysaccharides					749:763	the Sanchi flower polysaccharides	731:763	the Sanchi flower polysaccharides	731:763	NMR and FTIR analyses were indicative of the successful phosphorylation of the Sanchi flower polysaccharides.
36276535	5	29	theme	pore-like	848:856	arg1	structures					858:867	pore-like structures	848:867	pore-like structures	848:867	The introduction of phosphate groups into polysaccharides led to the induction of pore-like structures in polysaccharides configuration.
36276535	5	30	theme	phosphate	786:794	arg1	groups					796:801	phosphate groups	786:801	phosphate groups	786:801	The introduction of phosphate groups into polysaccharides led to the induction of pore-like structures in polysaccharides configuration.
36276535	2	31	from	GluN	481:484	arg1	compositions					514:525	their compositions	508:525	their compositions	508:525	Sanchi flower phosphorylated polysaccharides mainly comprised of Man, Rha, GluA, GalA, Glu, Gal and Xyl, but lacked GluN, Rib, Arab and Fuc in their compositions.
36276535	5	32	theme	structures	858:867	arg1	induction					835:843	the induction	831:843	the induction of pore-like structures in polysaccharides configuration	831:900	The introduction of phosphate groups into polysaccharides led to the induction of pore-like structures in polysaccharides configuration.
36276535	0	33	theme	structural	31:40	arg1	analysis					42:49	structural analysis	31:49	structural analysis	31:49	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	1	34	theme	flower	323:328	arg1	polysaccharides					330:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	Sanchi (Panax notoginseng) flower polysaccharides	296:344	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	5	35	theme	groups	796:801	arg1	introduction					770:781	The introduction	766:781	The introduction of phosphate groups into polysaccharides	766:822	The introduction of phosphate groups into polysaccharides led to the induction of pore-like structures in polysaccharides configuration.
36276535	3	36	theme	spectral	626:633	arg1	region					635:640	spectral region	626:640	spectral region of 1254 cm-1	626:653	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36276535	1	37	theme	structural	225:234	arg1	attributes					236:245	structural attributes	225:245	structural attributes	225:245	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	0	38	theme	antioxidant	55:65	arg1	activities					67:76	antioxidant activities	55:76	antioxidant activities	55:76	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	1	39	theme	polysaccharides	330:344	arg1	morphology					248:257	morphology	248:257	morphology	248:257	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	39	theme	polysaccharides	330:344	arg1	composition					212:222	the monosaccharide composition	193:222	the monosaccharide composition	193:222	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	39	theme	polysaccharides	330:344	arg1	attributes					236:245	structural attributes	225:245	structural attributes	225:245	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	1	39	theme	polysaccharides	330:344	arg1	activities					282:291	radical-scavenging activities	263:291	radical-scavenging activities	263:291	In this study, phosphorylation effects on the monosaccharide composition, structural attributes, morphology and radical-scavenging activities of Sanchi (Panax notoginseng) flower polysaccharides were investigated.
36276535	2	40	from	Rib	487:489	arg1	compositions					514:525	their compositions	508:525	their compositions	508:525	Sanchi flower phosphorylated polysaccharides mainly comprised of Man, Rha, GluA, GalA, Glu, Gal and Xyl, but lacked GluN, Rib, Arab and Fuc in their compositions.
36276535	4	41	theme	successful	701:710	arg1	phosphorylation					712:726	the successful phosphorylation	697:726	the successful phosphorylation of the Sanchi flower polysaccharides	697:763	NMR and FTIR analyses were indicative of the successful phosphorylation of the Sanchi flower polysaccharides.
36276535	7	42	theme	Sanchi	1095:1100	arg1	agent					1175:1179	a therapeutic agent	1161:1179	a therapeutic agent	1161:1179	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	7	42	theme	Sanchi	1095:1100	arg1	polysaccharides					1109:1123	Sanchi flower polysaccharides	1095:1123	Sanchi flower polysaccharides	1095:1123	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	6	43	theme	radical-scavenging	994:1011	arg1	activities					1013:1022	radical-scavenging activities	994:1022	radical-scavenging activities	994:1022	Phosphorylation of polysaccharides led to concentration-dependent increasing tendencies in radical-scavenging activities.
36276535	2	44	from	Arab	492:495	arg1	compositions					514:525	their compositions	508:525	their compositions	508:525	Sanchi flower phosphorylated polysaccharides mainly comprised of Man, Rha, GluA, GalA, Glu, Gal and Xyl, but lacked GluN, Rib, Arab and Fuc in their compositions.
36276535	3	45	theme	peak	614:617	arg1	emergence					586:594	an emergence	583:594	an emergence of new absorption peak around spectral region of 1254 cm-1	583:653	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36276535	7	46	theme	flower	1102:1107	arg1	agent					1175:1179	a therapeutic agent	1161:1179	a therapeutic agent	1161:1179	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	7	46	theme	flower	1102:1107	arg1	polysaccharides					1109:1123	Sanchi flower polysaccharides	1095:1123	Sanchi flower polysaccharides	1095:1123	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	2	47	theme	Sanchi	365:370	arg1	flower					372:377	Sanchi flower	365:377	Sanchi flower	365:377	Sanchi flower phosphorylated polysaccharides mainly comprised of Man, Rha, GluA, GalA, Glu, Gal and Xyl, but lacked GluN, Rib, Arab and Fuc in their compositions.
36276535	7	48	theme	phosphorylation	1076:1090	arg1	impact					1066:1071	the positive impact	1053:1071	the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent	1053:1179	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	4	49	theme	phosphorylation	712:726	arg1	indicative					683:692	indicative	683:692	indicative	683:692	NMR and FTIR analyses were indicative of the successful phosphorylation of the Sanchi flower polysaccharides.
36276535	0	50	from	flower	143:148	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	0	50	from	flower	143:148	arg1	analysis					42:49	structural analysis	31:49	structural analysis	31:49	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	0	50	from	flower	143:148	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	0	50	from	flower	143:148	arg1	activities					67:76	antioxidant activities	55:76	antioxidant activities	55:76	Preparation, characterization, structural analysis and antioxidant activities of phosphorylated polysaccharide from Sanchi (Panax notoginseng) flower.
36276535	2	51	from	Fuc	501:503	arg1	compositions					514:525	their compositions	508:525	their compositions	508:525	Sanchi flower phosphorylated polysaccharides mainly comprised of Man, Rha, GluA, GalA, Glu, Gal and Xyl, but lacked GluN, Rib, Arab and Fuc in their compositions.
36276535	3	52	theme	new	599:601	arg1	peak					614:617	new absorption peak	599:617	new absorption peak around spectral region of 1254 cm-1	599:653	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36276535	7	53	from	impact	1066:1071	arg1	agent					1175:1179	a therapeutic agent	1161:1179	a therapeutic agent	1161:1179	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	7	53	from	impact	1066:1071	arg1	polysaccharides					1109:1123	Sanchi flower polysaccharides	1095:1123	Sanchi flower polysaccharides	1095:1123	These findings demonstrated the positive impact of phosphorylation on Sanchi flower polysaccharides, which could potentially be used as a therapeutic agent.
36276535	3	54	theme	absorption	603:612	arg1	peak					614:617	new absorption peak	599:617	new absorption peak around spectral region of 1254 cm-1	599:653	FTIR analysis of phosphorylated polysaccharides showed an emergence of new absorption peak around spectral region of 1254 cm-1.
36515313	0	0	theme	reperfusion	92:102	arg1	injury					104:109	hepatic ischemia reperfusion injury	75:109	hepatic ischemia reperfusion injury in rats	75:117	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	0	1	from	effects	15:21	arg1	injury					104:109	hepatic ischemia reperfusion injury	75:109	hepatic ischemia reperfusion injury in rats	75:117	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	4	2	from	transaminase	543:554	arg1	serum					625:629	serum	625:629	serum	625:629	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	2	from	transaminase	543:554	arg1	feces					699:703	feces	699:703	feces	699:703	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	2	from	transaminase	543:554	arg1	composition					684:694	gut microbiota composition	669:694	gut microbiota composition	669:694	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	2	from	transaminase	543:554	arg1	SCFAs					657:661	SCFAs	657:661	SCFAs	657:661	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	2	from	transaminase	543:554	arg1	acids					650:654	short-chain fatty acids	632:654	short-chain fatty acids (SCFAs)	632:662	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	7	3	theme	clinical	1509:1516	arg1	butyricum					1483:1491	C. butyricum	1480:1491	C. butyricum	1480:1491	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	7	3	theme	clinical	1509:1516	arg1	supplement					1539:1548	a potent clinical preoperative dietary supplement	1500:1548	a potent clinical preoperative dietary supplement for HIRI	1500:1557	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	0	4	theme	ischemia	83:90	arg1	injury					104:109	hepatic ischemia reperfusion injury	75:109	hepatic ischemia reperfusion injury in rats	75:117	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	5	5	dep	RESULTS	928:934	arg1	showed					964:969	showed	964:969	showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics	964:1264	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	1	6	theme	oral	167:170	arg1	administration					172:185	oral administration	167:185	oral administration of Clostridium butyricum (C. butyricum)	167:225	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	7	theme	histological	877:888	arg1	analysis					890:897	histological analysis	877:897	histological analysis in the liver	877:910	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	5	8	theme	C.	951:952	arg1	butyricum					954:962	C. butyricum	951:962	C. butyricum	951:962	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	9	theme	probiotics	1255:1264	arg1	RA					1248:1249	RA	1248:1249	RA	1248:1249	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	9	theme	probiotics	1255:1264	arg1	abundance					1237:1245	the relative abundance	1224:1245	the relative abundance (RA) of probiotics	1224:1264	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	6	10	theme	inflammation	1430:1441	arg1	LPS					1407:1409	the decreased LPS	1393:1409	the decreased LPS	1393:1409	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	6	10	theme	inflammation	1430:1441	arg1	attenuation					1415:1425	attenuation	1415:1425	attenuation of inflammation and oxidative stress	1415:1462	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	5	11	from	changes	1043:1049	arg1	SCFAs					1054:1058	SCFAs	1054:1058	SCFAs	1054:1058	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	4	12	from	lipopolysaccharide	597:614	arg1	serum					625:629	serum	625:629	serum	625:629	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	12	from	lipopolysaccharide	597:614	arg1	feces					699:703	feces	699:703	feces	699:703	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	12	from	lipopolysaccharide	597:614	arg1	composition					684:694	gut microbiota composition	669:694	gut microbiota composition	669:694	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	12	from	lipopolysaccharide	597:614	arg1	SCFAs					657:661	SCFAs	657:661	SCFAs	657:661	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	12	from	lipopolysaccharide	597:614	arg1	acids					650:654	short-chain fatty acids	632:654	short-chain fatty acids (SCFAs)	632:662	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	5	13	theme	microbial	1136:1144	arg1	composition					1146:1156	the gut microbial composition	1128:1156	the gut microbial composition	1128:1156	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	2	14	dep	METHODS	333:339	arg1	given					379:383	given	379:383	were given C. butyricum for 5 days	374:407	METHODS The rats from C. butyricum group were given C. butyricum for 5 days.
36515313	1	15	theme	gut	266:268	arg1	flora					270:274	gut flora	266:274	gut flora	266:274	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	5	16	dep	ALT	981:983	arg1	changes					1043:1049	changes	1043:1049	changes in SCFAs	1043:1058	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	16	dep	ALT	981:983	arg1	GSH					1014:1016	improved GSH	1005:1016	improved GSH	1005:1016	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	16	dep	ALT	981:983	arg1	damage					1035:1040	histological damage	1022:1040	histological damage	1022:1040	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	17	theme	relative	1228:1235	arg1	RA					1248:1249	RA	1248:1249	RA	1248:1249	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	17	theme	relative	1228:1235	arg1	abundance					1237:1245	the relative abundance	1224:1245	the relative abundance (RA) of probiotics	1224:1264	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	1	18	from	effects	156:162	arg1	flora					270:274	gut flora	266:274	gut flora	266:274	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	18	from	effects	156:162	arg1	inflammation					230:241	inflammation	230:241	inflammation	230:241	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	18	from	effects	156:162	arg1	stress					254:259	oxidative stress	244:259	oxidative stress	244:259	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	19	dep	PURPOSE	120:126	arg1	investigated					139:150	investigated	139:150	investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI)	139:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	2	20	theme	butyricum	358:366	arg1	group					368:372	C. butyricum group	355:372	C. butyricum group	355:372	METHODS The rats from C. butyricum group were given C. butyricum for 5 days.
36515313	1	21	theme	administration	172:185	arg1	effects					156:162	the effects	152:162	the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI)	152:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	22	theme	sacrificed	523:532	arg1	MDA					727:729	MDA	727:729	MDA	727:729	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	22	theme	sacrificed	523:532	arg1	glutathione					733:743	glutathione	733:743	glutathione (GSH)	733:749	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	22	theme	sacrificed	523:532	arg1	factor-α					767:774	tumor necrosis factor-α	752:774	tumor necrosis factor-α	752:774	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	22	theme	sacrificed	523:532	arg1	transaminase					543:554	alanine transaminase	535:554	alanine transaminase (ALT)	535:560	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	22	theme	sacrificed	523:532	arg1	ALT					557:559	ALT	557:559	ALT	557:559	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	22	theme	sacrificed	523:532	arg1	animals					510:516	the animals	506:516	the animals	506:516	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	1	23	with	rats	279:282	arg1	HIRI					326:329	HIRI	326:329	HIRI	326:329	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	23	with	rats	279:282	arg1	injury					318:323	hepatic ischemia reperfusion injury	289:323	hepatic ischemia reperfusion injury (HIRI)	289:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	24	from	receptor	817:824	arg1	liver					906:910	the liver	902:910	the liver	902:910	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	6	25	theme	microbial	1349:1357	arg1	composition					1359:1369	gut microbial composition	1345:1369	gut microbial composition	1345:1369	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	0	26	theme	protective	4:13	arg1	effects					15:21	The protective effects	0:21	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.	0:118	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	5	27	dep	showed	964:969	arg1	decreased					971:979	decreased	971:979	showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics	964:1264	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	1	28	from	flora	270:274	arg1	rats					279:282	rats	279:282	rats with hepatic ischemia reperfusion injury (HIRI)	279:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	29	theme	Toll-like	807:815	arg1	receptor					817:824	Toll-like receptor 4	807:826	Toll-like receptor 4 (TLR4)	807:833	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	29	theme	Toll-like	807:815	arg1	TLR4					829:832	TLR4	829:832	TLR4	829:832	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	7	30	theme	C.	1480:1481	arg1	supplement					1539:1548	a potent clinical preoperative dietary supplement	1500:1548	a potent clinical preoperative dietary supplement for HIRI	1500:1557	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	7	30	theme	C.	1480:1481	arg1	butyricum					1483:1491	C. butyricum	1480:1491	C. butyricum	1480:1491	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	1	31	theme	Clostridium	190:200	arg1	butyricum					202:210	Clostridium butyricum	190:210	Clostridium butyricum (C. butyricum)	190:225	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	31	theme	Clostridium	190:200	arg1	butyricum					216:224	C. butyricum	213:224	C. butyricum	213:224	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	32	theme	nuclear	836:842	arg1	NF-κBp65					863:870	NF-κBp65	863:870	NF-κBp65	863:870	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	32	theme	nuclear	836:842	arg1	Bp65					857:860	nuclear factor-kappa Bp65	836:860	nuclear factor-kappa Bp65 (NF-κBp65)	836:871	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	6	33	theme	oxidative	1447:1455	arg1	stress					1457:1462	oxidative stress	1447:1462	oxidative stress	1447:1462	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	6	34	theme	decreased	1397:1405	arg1	LPS					1407:1409	the decreased LPS	1393:1409	the decreased LPS	1393:1409	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	4	35	theme	fatty	644:648	arg1	SCFAs					657:661	SCFAs	657:661	SCFAs	657:661	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	35	theme	fatty	644:648	arg1	acids					650:654	short-chain fatty acids	632:654	short-chain fatty acids (SCFAs)	632:662	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	36	from	aminotransferase	573:588	arg1	serum					625:629	serum	625:629	serum	625:629	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	36	from	aminotransferase	573:588	arg1	feces					699:703	feces	699:703	feces	699:703	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	36	from	aminotransferase	573:588	arg1	composition					684:694	gut microbiota composition	669:694	gut microbiota composition	669:694	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	36	from	aminotransferase	573:588	arg1	SCFAs					657:661	SCFAs	657:661	SCFAs	657:661	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	36	from	aminotransferase	573:588	arg1	acids					650:654	short-chain fatty acids	632:654	short-chain fatty acids (SCFAs)	632:662	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	1	37	theme	butyricum	202:210	arg1	administration					172:185	oral administration	167:185	oral administration of Clostridium butyricum (C. butyricum)	167:225	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	38	from	Bp65	857:860	arg1	liver					906:910	the liver	902:910	the liver	902:910	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	5	39	theme	Firmicutes/Bacteroidetes	1179:1202	arg1	ratio					1204:1208	the Firmicutes/Bacteroidetes ratio	1175:1208	the Firmicutes/Bacteroidetes ratio	1175:1208	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	6	40	theme	butyricum	1282:1290	arg1	supplementation					1292:1306	CONCLUSIONS C. butyricum supplementation	1267:1306	CONCLUSIONS C. butyricum supplementation	1267:1306	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	5	41	from	TNF-α	1070:1074	arg1	composition					1146:1156	the gut microbial composition	1128:1156	the gut microbial composition	1128:1156	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	4	42	theme	gut	669:671	arg1	composition					684:694	gut microbiota composition	669:694	gut microbiota composition	669:694	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	43	from	interleukin	785:795	arg1	liver					906:910	the liver	902:910	the liver	902:910	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	6	44	theme	CONCLUSIONS	1267:1277	arg1	supplementation					1292:1306	CONCLUSIONS C. butyricum supplementation	1267:1306	CONCLUSIONS C. butyricum supplementation	1267:1306	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	6	45	theme	C.	1279:1280	arg1	supplementation					1292:1306	CONCLUSIONS C. butyricum supplementation	1267:1306	CONCLUSIONS C. butyricum supplementation	1267:1306	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	1	46	theme	C.	213:214	arg1	butyricum					202:210	Clostridium butyricum	190:210	Clostridium butyricum (C. butyricum)	190:225	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	46	theme	C.	213:214	arg1	butyricum					216:224	C. butyricum	213:224	C. butyricum	213:224	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	0	47	theme	Clostridium	34:44	arg1	supplementation					56:70	dietary Clostridium butyricum supplementation	26:70	dietary Clostridium butyricum supplementation	26:70	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	1	48	from	stress	254:259	arg1	rats					279:282	rats	279:282	rats with hepatic ischemia reperfusion injury (HIRI)	279:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	49	theme	aspartate	563:571	arg1	AST					591:593	AST	591:593	AST	591:593	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	49	theme	aspartate	563:571	arg1	aminotransferase					573:588	aspartate aminotransferase	563:588	aspartate aminotransferase (AST)	563:594	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	2	50	theme	C.	355:356	arg1	group					368:372	C. butyricum group	355:372	C. butyricum group	355:372	METHODS The rats from C. butyricum group were given C. butyricum for 5 days.
36515313	2	51	theme	C.	385:386	arg1	butyricum					388:396	C. butyricum	385:396	C. butyricum	385:396	METHODS The rats from C. butyricum group were given C. butyricum for 5 days.
36515313	0	52	theme	dietary	26:32	arg1	supplementation					56:70	dietary Clostridium butyricum supplementation	26:70	dietary Clostridium butyricum supplementation	26:70	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	1	53	theme	hepatic	289:295	arg1	HIRI					326:329	HIRI	326:329	HIRI	326:329	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	53	theme	hepatic	289:295	arg1	injury					318:323	hepatic ischemia reperfusion injury	289:323	hepatic ischemia reperfusion injury (HIRI)	289:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	5	54	from	TLR4	1083:1086	arg1	composition					1146:1156	the gut microbial composition	1128:1156	the gut microbial composition	1128:1156	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	55	theme	gut	1132:1134	arg1	composition					1146:1156	the gut microbial composition	1128:1156	the gut microbial composition	1128:1156	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	3	56	theme	hepatic	416:422	arg1	ischemia					424:431	hepatic ischemia	416:431	hepatic ischemia for 30 min	416:442	Then, hepatic ischemia for 30 min and reperfusion for 6 h were performed in all the rats.
36515313	0	57	theme	supplementation	56:70	arg1	effects					15:21	The protective effects	0:21	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.	0:118	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	1	58	theme	ischemia	297:304	arg1	HIRI					326:329	HIRI	326:329	HIRI	326:329	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	58	theme	ischemia	297:304	arg1	injury					318:323	hepatic ischemia reperfusion injury	289:323	hepatic ischemia reperfusion injury (HIRI)	289:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	59	theme	necrosis	758:765	arg1	factor-α					767:774	tumor necrosis factor-α	752:774	tumor necrosis factor-α	752:774	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	59	theme	necrosis	758:765	arg1	transaminase					543:554	alanine transaminase	535:554	alanine transaminase (ALT)	535:560	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	60	from	malondialdehyde	710:724	arg1	serum					625:629	serum	625:629	serum	625:629	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	60	from	malondialdehyde	710:724	arg1	feces					699:703	feces	699:703	feces	699:703	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	60	from	malondialdehyde	710:724	arg1	composition					684:694	gut microbiota composition	669:694	gut microbiota composition	669:694	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	60	from	malondialdehyde	710:724	arg1	SCFAs					657:661	SCFAs	657:661	SCFAs	657:661	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	60	from	malondialdehyde	710:724	arg1	acids					650:654	short-chain fatty acids	632:654	short-chain fatty acids (SCFAs)	632:662	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	0	61	theme	butyricum	46:54	arg1	supplementation					56:70	dietary Clostridium butyricum supplementation	26:70	dietary Clostridium butyricum supplementation	26:70	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	1	62	theme	reperfusion	306:316	arg1	HIRI					326:329	HIRI	326:329	HIRI	326:329	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	1	62	theme	reperfusion	306:316	arg1	injury					318:323	hepatic ischemia reperfusion injury	289:323	hepatic ischemia reperfusion injury (HIRI)	289:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	63	theme	tumor	752:756	arg1	factor-α					767:774	tumor necrosis factor-α	752:774	tumor necrosis factor-α	752:774	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	63	theme	tumor	752:756	arg1	transaminase					543:554	alanine transaminase	535:554	alanine transaminase (ALT)	535:560	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	5	64	from	IL-6	1077:1080	arg1	composition					1146:1156	the gut microbial composition	1128:1156	the gut microbial composition	1128:1156	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	5	65	from	pNF-κBp65/NF-κBp65	1093:1110	arg1	composition					1146:1156	the gut microbial composition	1128:1156	the gut microbial composition	1128:1156	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	2	66	from	group	368:372	arg1	rats					345:348	The rats	341:348	The rats from C. butyricum group	341:372	METHODS The rats from C. butyricum group were given C. butyricum for 5 days.
36515313	4	67	theme	short-chain	632:642	arg1	SCFAs					657:661	SCFAs	657:661	SCFAs	657:661	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	67	theme	short-chain	632:642	arg1	acids					650:654	short-chain fatty acids	632:654	short-chain fatty acids (SCFAs)	632:662	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	5	68	theme	improved	1005:1012	arg1	GSH					1014:1016	improved GSH	1005:1016	improved GSH	1005:1016	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	1	69	from	inflammation	230:241	arg1	rats					279:282	rats	279:282	rats with hepatic ischemia reperfusion injury (HIRI)	279:330	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36515313	4	70	dep	transaminase	543:554	arg1	TNF-α					777:781	TNF-α	777:781	TNF-α	777:781	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	71	theme	microbiota	673:682	arg1	composition					684:694	gut microbiota composition	669:694	gut microbiota composition	669:694	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	72	from	analysis	890:897	arg1	liver					906:910	the liver	902:910	the liver	902:910	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	7	73	theme	preoperative	1518:1529	arg1	butyricum					1483:1491	C. butyricum	1480:1491	C. butyricum	1480:1491	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	7	73	theme	preoperative	1518:1529	arg1	supplement					1539:1548	a potent clinical preoperative dietary supplement	1500:1548	a potent clinical preoperative dietary supplement for HIRI	1500:1557	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	4	74	theme	alanine	535:541	arg1	MDA					727:729	MDA	727:729	MDA	727:729	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	74	theme	alanine	535:541	arg1	glutathione					733:743	glutathione	733:743	glutathione (GSH)	733:749	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	74	theme	alanine	535:541	arg1	factor-α					767:774	tumor necrosis factor-α	752:774	tumor necrosis factor-α	752:774	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	74	theme	alanine	535:541	arg1	transaminase					543:554	alanine transaminase	535:554	alanine transaminase (ALT)	535:560	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	74	theme	alanine	535:541	arg1	ALT					557:559	ALT	557:559	ALT	557:559	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	74	theme	alanine	535:541	arg1	animals					510:516	the animals	506:516	the animals	506:516	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	0	75	theme	hepatic	75:81	arg1	injury					104:109	hepatic ischemia reperfusion injury	75:109	hepatic ischemia reperfusion injury in rats	75:117	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	0	76	from	injury	104:109	arg1	rats					114:117	rats	114:117	rats	114:117	The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.
36515313	5	77	from	changes	1117:1123	arg1	composition					1146:1156	the gut microbial composition	1128:1156	the gut microbial composition	1128:1156	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	7	78	theme	dietary	1531:1537	arg1	butyricum					1483:1491	C. butyricum	1480:1491	C. butyricum	1480:1491	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	7	78	theme	dietary	1531:1537	arg1	supplement					1539:1548	a potent clinical preoperative dietary supplement	1500:1548	a potent clinical preoperative dietary supplement for HIRI	1500:1557	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	6	79	theme	stress	1457:1462	arg1	LPS					1407:1409	the decreased LPS	1393:1409	the decreased LPS	1393:1409	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	6	79	theme	stress	1457:1462	arg1	attenuation					1415:1425	attenuation	1415:1425	attenuation of inflammation and oxidative stress	1415:1462	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	5	80	theme	histological	1022:1033	arg1	damage					1035:1040	histological damage	1022:1040	histological damage	1022:1040	RESULTS The rats given C. butyricum showed decreased ALT, AST, LPS, and MDA; improved GSH and histological damage; changes in SCFAs; declined TNF-α, IL-6, TLR4, and pNF-κBp65/NF-κBp65; and changes in the gut microbial composition, which decreased the Firmicutes/Bacteroidetes ratio and increased the relative abundance (RA) of probiotics.
36515313	6	81	theme	gut	1345:1347	arg1	composition					1359:1369	gut microbial composition	1345:1369	gut microbial composition	1345:1369	CONCLUSIONS C. butyricum supplementation protected against HIRI by regulating gut microbial composition, which contributed to the decreased LPS and attenuation of inflammation and oxidative stress.
36515313	4	82	theme	factor-kappa	844:855	arg1	NF-κBp65					863:870	NF-κBp65	863:870	NF-κBp65	863:870	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	4	82	theme	factor-kappa	844:855	arg1	Bp65					857:860	nuclear factor-kappa Bp65	836:860	nuclear factor-kappa Bp65 (NF-κBp65)	836:871	After the animals were sacrificed, alanine transaminase (ALT), aspartate aminotransferase (AST), lipopolysaccharide (LPS) in serum, short-chain fatty acids (SCFAs), and gut microbiota composition in feces, and malondialdehyde (MDA), glutathione (GSH), tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), Toll-like receptor 4 (TLR4), nuclear factor-kappa Bp65 (NF-κBp65) and histological analysis in the liver were performed.
36515313	7	83	theme	potent	1502:1507	arg1	butyricum					1483:1491	C. butyricum	1480:1491	C. butyricum	1480:1491	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	7	83	theme	potent	1502:1507	arg1	supplement					1539:1548	a potent clinical preoperative dietary supplement	1500:1548	a potent clinical preoperative dietary supplement for HIRI	1500:1557	These indicate C. butyricum may be a potent clinical preoperative dietary supplement for HIRI.
36515313	1	84	theme	oxidative	244:252	arg1	stress					254:259	oxidative stress	244:259	oxidative stress	244:259	PURPOSE This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).
36838708	6	0	theme	FA	933:934	arg1	supplementation					936:950	FA supplementation	933:950	FA supplementation	933:950	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	2	1	theme	intestinal	372:381	arg1	composition					393:403	intestinal microbial composition	372:403	intestinal microbial composition	372:403	Previous studies have demonstrated that LPS impaired gastrointestinal development and disrupted intestinal microbial composition and metabolism.
36838708	9	2	from	composition	1575:1585	arg1	broilers					1605:1612	LPS-challenged broilers	1590:1612	LPS-challenged broilers	1590:1612	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	1	3	theme	stress	250:255	arg1	models					212:217	animal models	205:217	animal models of inflammation, immunological stress, and organ injury	205:273	Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury.
36838708	4	4	theme	intestinal	557:566	arg1	function					568:575	intestinal function	557:575	intestinal function	557:575	This study investigated whether FA ameliorated intestinal function and microflora in LPS-challenged Tianfu broilers.
36838708	5	5	theme	decreased	711:719	arg1	<					746:746	p < 0.05	744:751	p < 0.05	744:751	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	5	theme	decreased	711:719	arg1	functions					733:741	decreased antioxidant functions	711:741	decreased antioxidant functions (p < 0.05)	711:752	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	6	theme	LPS	651:653	arg1	challenge					655:663	LPS challenge	651:663	LPS challenge	651:663	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	6	7	theme	enhanced	1052:1059	arg1	proliferation					1072:1084	enhanced epithelial proliferation	1052:1084	enhanced epithelial proliferation (p < 0.05)	1052:1095	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	7	theme	enhanced	1052:1059	arg1	<					1089:1089	p < 0.05	1087:1094	p < 0.05	1087:1094	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	8	theme	mRNA	996:999	arg1	<					1039:1039	p < 0.05	1037:1044	p < 0.05	1037:1044	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	8	theme	mRNA	996:999	arg1	levels					1012:1017	increased mRNA expression levels	986:1017	increased mRNA expression levels of CLDN and ZO-1 (p < 0.05)	986:1045	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	0	9	theme	Broilers	84:91	arg1	Microbiota					63:72	Ileal Microbiota	57:72	Ileal Microbiota	57:72	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	0	9	theme	Broilers	84:91	arg1	Health					46:51	Intestinal Health	35:51	Intestinal Health	35:51	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	0	10	from	Effects	0:6	arg1	Microbiota					63:72	Ileal Microbiota	57:72	Ileal Microbiota	57:72	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	0	10	from	Effects	0:6	arg1	Health					46:51	Intestinal Health	35:51	Intestinal Health	35:51	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	5	11	theme	antioxidant	721:731	arg1	<					746:746	p < 0.05	744:751	p < 0.05	744:751	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	11	theme	antioxidant	721:731	arg1	functions					733:741	decreased antioxidant functions	711:741	decreased antioxidant functions (p < 0.05)	711:752	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	9	12	theme	microflora	1564:1573	arg1	composition					1575:1585	ileal microflora composition	1558:1585	ileal microflora composition in LPS-challenged broilers	1558:1612	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	6	13	theme	increased	986:994	arg1	<					1039:1039	p < 0.05	1037:1044	p < 0.05	1037:1044	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	13	theme	increased	986:994	arg1	levels					1012:1017	increased mRNA expression levels	986:1017	increased mRNA expression levels of CLDN and ZO-1 (p < 0.05)	986:1045	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	0	14	theme	Tianfu	77:82	arg1	Broilers					84:91	Tianfu Broilers	77:91	Tianfu Broilers	77:91	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	9	15	theme	ileal	1558:1562	arg1	composition					1575:1585	ileal microflora composition	1558:1585	ileal microflora composition in LPS-challenged broilers	1558:1612	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	2	16	theme	Previous	276:283	arg1	studies					285:291	Previous studies	276:291	Previous studies	276:291	Previous studies have demonstrated that LPS impaired gastrointestinal development and disrupted intestinal microbial composition and metabolism.
36838708	9	17	theme	intestinal	1459:1468	arg1	TJs					1487:1489	TJs	1487:1489	TJs	1487:1489	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	9	17	theme	intestinal	1459:1468	arg1	junctions					1476:1484	intestinal tight junctions	1459:1484	intestinal tight junctions (TJs)	1459:1490	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	1	18	theme	organ	262:266	arg1	injury					268:273	organ injury	262:273	organ injury	262:273	Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury.
36838708	5	19	theme	p	790:790	arg1	structure					779:787	disrupted morphological structure	755:787	disrupted morphological structure (p < 0.05)	755:798	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	19	theme	p	790:790	arg1	<					792:792	p < 0.05	790:797	p < 0.05	790:797	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	6	20	theme	epithelial	1061:1070	arg1	proliferation					1072:1084	enhanced epithelial proliferation	1052:1084	enhanced epithelial proliferation (p < 0.05)	1052:1095	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	20	theme	epithelial	1061:1070	arg1	<					1089:1089	p < 0.05	1087:1094	p < 0.05	1087:1094	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	5	21	theme	intestinal	674:683	arg1	function					685:692	intestinal function	674:692	intestinal function	674:692	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	6	22	theme	LPS-challenged	1120:1133	arg1	chickens					1135:1142	LPS-challenged chickens	1120:1142	LPS-challenged chickens	1120:1142	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	3	23	theme	multiple	453:460	arg1	plants					462:467	multiple plants	453:467	multiple plants	453:467	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	4	24	from	function	568:575	arg1	broilers					617:624	LPS-challenged Tianfu broilers	595:624	LPS-challenged Tianfu broilers	595:624	This study investigated whether FA ameliorated intestinal function and microflora in LPS-challenged Tianfu broilers.
36838708	6	25	theme	expression	1001:1010	arg1	<					1039:1039	p < 0.05	1037:1044	p < 0.05	1037:1044	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	25	theme	expression	1001:1010	arg1	levels					1012:1017	increased mRNA expression levels	986:1017	increased mRNA expression levels of CLDN and ZO-1 (p < 0.05)	986:1045	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	26	theme	CLDN	1022:1025	arg1	<					1089:1089	p < 0.05	1087:1094	p < 0.05	1087:1094	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	26	theme	CLDN	1022:1025	arg1	<					977:977	p < 0.05	975:982	p < 0.05	975:982	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	26	theme	CLDN	1022:1025	arg1	<					1039:1039	p < 0.05	1037:1044	p < 0.05	1037:1044	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	26	theme	CLDN	1022:1025	arg1	levels					1012:1017	increased mRNA expression levels	986:1017	increased mRNA expression levels of CLDN and ZO-1 (p < 0.05)	986:1045	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	26	theme	CLDN	1022:1025	arg1	proliferation					1072:1084	enhanced epithelial proliferation	1052:1084	enhanced epithelial proliferation (p < 0.05)	1052:1095	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	26	theme	CLDN	1022:1025	arg1	levels					967:972	sIgA levels	962:972	sIgA levels (p < 0.05)	962:983	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	5	27	theme	p	840:840	arg1	permeability					826:837	increased intestinal permeability	805:837	increased intestinal permeability (p < 0.05)	805:848	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	27	theme	p	840:840	arg1	<					842:842	p < 0.05	840:847	p < 0.05	840:847	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	4	28	theme	Tianfu	610:615	arg1	broilers					617:624	LPS-challenged Tianfu broilers	595:624	LPS-challenged Tianfu broilers	595:624	This study investigated whether FA ameliorated intestinal function and microflora in LPS-challenged Tianfu broilers.
36838708	1	29	theme	primary	178:184	arg1	agent					186:190	the primary agent	174:190	the primary agent to establish animal models of inflammation, immunological stress, and organ injury	174:273	Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury.
36838708	3	30	theme	Ferulic	421:427	arg1	FA					435:436	FA	435:436	FA	435:436	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	3	30	theme	Ferulic	421:427	arg1	acid					429:432	Ferulic acid	421:432	Ferulic acid (FA) isolated from multiple plants	421:467	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	1	31	theme	injury	268:273	arg1	models					212:217	animal models	205:217	animal models of inflammation, immunological stress, and organ injury	205:273	Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury.
36838708	5	32	theme	adverse	866:872	arg1	effects					874:880	these adverse effects	860:880	these adverse effects	860:880	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	6	33	theme	ileal	1104:1108	arg1	mucosa					1110:1115	the ileal mucosa	1100:1115	the ileal mucosa in LPS-challenged chickens	1100:1142	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	0	34	theme	Ferulic	19:25	arg1	Acid					27:30	Dietary Ferulic Acid	11:30	Dietary Ferulic Acid	11:30	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	4	35	theme	LPS-challenged	595:608	arg1	broilers					617:624	LPS-challenged Tianfu broilers	595:624	LPS-challenged Tianfu broilers	595:624	This study investigated whether FA ameliorated intestinal function and microflora in LPS-challenged Tianfu broilers.
36838708	8	36	theme	dietary	1279:1285	arg1	supplementation					1290:1304	dietary FA supplementation	1279:1304	dietary FA supplementation	1279:1304	The results demonstrate that dietary FA supplementation decreased LPS-induced intestinal damage by enhancing antioxidant capacity and maintaining intestinal integrity.
36838708	3	37	attach	isolated	439:446	arg1	plants					462:467	multiple plants	453:467	multiple plants	453:467	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	3	37	attach	isolated	439:446	arg2	acid					429:432	Ferulic acid	421:432	Ferulic acid (FA) isolated from multiple plants	421:467	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	3	37	attach	isolated	439:446	arg2	FA					435:436	FA	435:436	FA	435:436	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	8	38	theme	intestinal	1396:1405	arg1	integrity					1407:1415	intestinal integrity	1396:1415	intestinal integrity	1396:1415	The results demonstrate that dietary FA supplementation decreased LPS-induced intestinal damage by enhancing antioxidant capacity and maintaining intestinal integrity.
36838708	7	39	from	disturbances	1205:1216	arg1	broilers					1240:1247	the LPS-challenged broilers	1221:1247	the LPS-challenged broilers	1221:1247	Moreover, FA supplementation rectified the ileal microflora disturbances in the LPS-challenged broilers.
36838708	3	40	theme	biological	487:496	arg1	activities					498:507	multiple biological activities	478:507	multiple biological activities	478:507	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	0	41	theme	Dietary	11:17	arg1	Acid					27:30	Dietary Ferulic Acid	11:30	Dietary Ferulic Acid	11:30	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	8	42	theme	antioxidant	1359:1369	arg1	capacity					1371:1378	antioxidant capacity	1359:1378	antioxidant capacity	1359:1378	The results demonstrate that dietary FA supplementation decreased LPS-induced intestinal damage by enhancing antioxidant capacity and maintaining intestinal integrity.
36838708	8	43	theme	LPS-induced	1316:1326	arg1	damage					1339:1344	LPS-induced intestinal damage	1316:1344	LPS-induced intestinal damage	1316:1344	The results demonstrate that dietary FA supplementation decreased LPS-induced intestinal damage by enhancing antioxidant capacity and maintaining intestinal integrity.
36838708	6	44	from	mucosa	1110:1115	arg1	chickens					1135:1142	LPS-challenged chickens	1120:1142	LPS-challenged chickens	1120:1142	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	45	theme	p	1037:1037	arg1	<					1039:1039	p < 0.05	1037:1044	p < 0.05	1037:1044	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	45	theme	p	1037:1037	arg1	levels					1012:1017	increased mRNA expression levels	986:1017	increased mRNA expression levels of CLDN and ZO-1 (p < 0.05)	986:1045	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	5	46	theme	increased	805:813	arg1	permeability					826:837	increased intestinal permeability	805:837	increased intestinal permeability (p < 0.05)	805:848	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	46	theme	increased	805:813	arg1	<					842:842	p < 0.05	840:847	p < 0.05	840:847	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	6	47	theme	p	975:975	arg1	levels					967:972	sIgA levels	962:972	sIgA levels (p < 0.05)	962:983	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	47	theme	p	975:975	arg1	<					977:977	p < 0.05	975:982	p < 0.05	975:982	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	5	48	theme	disrupted	755:763	arg1	structure					779:787	disrupted morphological structure	755:787	disrupted morphological structure (p < 0.05)	755:798	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	48	theme	disrupted	755:763	arg1	<					792:792	p < 0.05	790:797	p < 0.05	790:797	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	49	theme	morphological	765:777	arg1	structure					779:787	disrupted morphological structure	755:787	disrupted morphological structure (p < 0.05)	755:798	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	49	theme	morphological	765:777	arg1	<					792:792	p < 0.05	790:797	p < 0.05	790:797	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	8	50	theme	FA	1287:1288	arg1	supplementation					1290:1304	dietary FA supplementation	1279:1304	dietary FA supplementation	1279:1304	The results demonstrate that dietary FA supplementation decreased LPS-induced intestinal damage by enhancing antioxidant capacity and maintaining intestinal integrity.
36838708	1	51	theme	animal	205:210	arg1	models					212:217	animal models	205:217	animal models of inflammation, immunological stress, and organ injury	205:273	Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury.
36838708	0	52	theme	Acid	27:30	arg1	Effects					0:6	Effects	0:6	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers	0:91	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	4	53	from	microflora	581:590	arg1	broilers					617:624	LPS-challenged Tianfu broilers	595:624	LPS-challenged Tianfu broilers	595:624	This study investigated whether FA ameliorated intestinal function and microflora in LPS-challenged Tianfu broilers.
36838708	9	54	theme	secretory	1502:1510	arg1	sIgA					1530:1533	sIgA	1530:1533	sIgA	1530:1533	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	9	54	theme	secretory	1502:1510	arg1	A					1527:1527	secretory immunoglobulin A	1502:1527	secretory immunoglobulin A (sIgA) levels	1502:1541	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	9	55	theme	FA	1431:1432	arg1	supplementation					1434:1448	FA supplementation	1431:1448	FA supplementation	1431:1448	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	3	56	theme	multiple	478:485	arg1	activities					498:507	multiple biological activities	478:507	multiple biological activities	478:507	Ferulic acid (FA) isolated from multiple plants exhibits multiple biological activities.
36838708	7	57	theme	FA	1155:1156	arg1	supplementation					1158:1172	FA supplementation	1155:1172	FA supplementation	1155:1172	Moreover, FA supplementation rectified the ileal microflora disturbances in the LPS-challenged broilers.
36838708	9	58	theme	tight	1470:1474	arg1	TJs					1487:1489	TJs	1487:1489	TJs	1487:1489	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	9	58	theme	tight	1470:1474	arg1	junctions					1476:1484	intestinal tight junctions	1459:1484	intestinal tight junctions (TJs)	1459:1490	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	9	59	theme	immunoglobulin	1512:1525	arg1	sIgA					1530:1533	sIgA	1530:1533	sIgA	1530:1533	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	9	59	theme	immunoglobulin	1512:1525	arg1	A					1527:1527	secretory immunoglobulin A	1502:1527	secretory immunoglobulin A (sIgA) levels	1502:1541	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	0	60	theme	Intestinal	35:44	arg1	Health					46:51	Intestinal Health	35:51	Intestinal Health	35:51	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	6	61	theme	sIgA	962:965	arg1	levels					967:972	sIgA levels	962:972	sIgA levels (p < 0.05)	962:983	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	61	theme	sIgA	962:965	arg1	<					977:977	p < 0.05	975:982	p < 0.05	975:982	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	5	62	theme	p	744:744	arg1	<					746:746	p < 0.05	744:751	p < 0.05	744:751	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	62	theme	p	744:744	arg1	functions					733:741	decreased antioxidant functions	711:741	decreased antioxidant functions (p < 0.05)	711:752	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	7	63	theme	ileal	1188:1192	arg1	disturbances					1205:1216	the ileal microflora disturbances	1184:1216	the ileal microflora disturbances in the LPS-challenged broilers	1184:1247	Moreover, FA supplementation rectified the ileal microflora disturbances in the LPS-challenged broilers.
36838708	7	64	theme	LPS-challenged	1225:1238	arg1	broilers					1240:1247	the LPS-challenged broilers	1221:1247	the LPS-challenged broilers	1221:1247	Moreover, FA supplementation rectified the ileal microflora disturbances in the LPS-challenged broilers.
36838708	2	65	theme	gastrointestinal	329:344	arg1	development					346:356	gastrointestinal development	329:356	gastrointestinal development	329:356	Previous studies have demonstrated that LPS impaired gastrointestinal development and disrupted intestinal microbial composition and metabolism.
36838708	6	66	theme	p	1087:1087	arg1	proliferation					1072:1084	enhanced epithelial proliferation	1052:1084	enhanced epithelial proliferation (p < 0.05)	1052:1095	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	66	theme	p	1087:1087	arg1	<					1089:1089	p < 0.05	1087:1094	p < 0.05	1087:1094	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	5	67	theme	FA	899:900	arg1	supplementation					902:916	FA supplementation	899:916	FA supplementation	899:916	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	8	68	theme	intestinal	1328:1337	arg1	damage					1339:1344	LPS-induced intestinal damage	1316:1344	LPS-induced intestinal damage	1316:1344	The results demonstrate that dietary FA supplementation decreased LPS-induced intestinal damage by enhancing antioxidant capacity and maintaining intestinal integrity.
36838708	1	69	theme	inflammation	222:233	arg1	models					212:217	animal models	205:217	animal models of inflammation, immunological stress, and organ injury	205:273	Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury.
36838708	0	70	theme	Ileal	57:61	arg1	Microbiota					63:72	Ileal Microbiota	57:72	Ileal Microbiota	57:72	Effects of Dietary Ferulic Acid on Intestinal Health and Ileal Microbiota of Tianfu Broilers Challenged with Lipopolysaccharide.
36838708	6	71	theme	ZO-1	1031:1034	arg1	<					1089:1089	p < 0.05	1087:1094	p < 0.05	1087:1094	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	71	theme	ZO-1	1031:1034	arg1	<					977:977	p < 0.05	975:982	p < 0.05	975:982	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	71	theme	ZO-1	1031:1034	arg1	<					1039:1039	p < 0.05	1037:1044	p < 0.05	1037:1044	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	71	theme	ZO-1	1031:1034	arg1	levels					1012:1017	increased mRNA expression levels	986:1017	increased mRNA expression levels of CLDN and ZO-1 (p < 0.05)	986:1045	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	71	theme	ZO-1	1031:1034	arg1	proliferation					1072:1084	enhanced epithelial proliferation	1052:1084	enhanced epithelial proliferation (p < 0.05)	1052:1095	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	6	71	theme	ZO-1	1031:1034	arg1	levels					967:972	sIgA levels	962:972	sIgA levels (p < 0.05)	962:983	Additionally, FA supplementation preserved sIgA levels (p < 0.05), increased mRNA expression levels of CLDN and ZO-1 (p < 0.05), and enhanced epithelial proliferation (p < 0.05) in the ileal mucosa in LPS-challenged chickens.
36838708	9	72	theme	A	1527:1527	arg1	levels					1536:1541	secretory immunoglobulin A (sIgA) levels	1502:1541	secretory immunoglobulin A (sIgA) levels	1502:1541	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	9	73	theme	LPS-challenged	1590:1603	arg1	broilers					1605:1612	LPS-challenged broilers	1590:1612	LPS-challenged broilers	1590:1612	Furthermore, FA supplementation protects intestinal tight junctions (TJs), elevates secretory immunoglobulin A (sIgA) levels, and modulates ileal microflora composition in LPS-challenged broilers.
36838708	7	74	theme	microflora	1194:1203	arg1	disturbances					1205:1216	the ileal microflora disturbances	1184:1216	the ileal microflora disturbances in the LPS-challenged broilers	1184:1247	Moreover, FA supplementation rectified the ileal microflora disturbances in the LPS-challenged broilers.
36838708	5	75	theme	intestinal	815:824	arg1	permeability					826:837	increased intestinal permeability	805:837	increased intestinal permeability (p < 0.05)	805:848	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	5	75	theme	intestinal	815:824	arg1	<					842:842	p < 0.05	840:847	p < 0.05	840:847	The results showed that LPS challenge impaired intestinal function, as evidenced by decreased antioxidant functions (p < 0.05), disrupted morphological structure (p < 0.05), and increased intestinal permeability (p < 0.05); however, these adverse effects were improved by FA supplementation.
36838708	2	76	theme	microbial	383:391	arg1	composition					393:403	intestinal microbial composition	372:403	intestinal microbial composition	372:403	Previous studies have demonstrated that LPS impaired gastrointestinal development and disrupted intestinal microbial composition and metabolism.
36838708	1	77	theme	immunological	236:248	arg1	stress					250:255	immunological stress	236:255	immunological stress	236:255	Lipopolysaccharide (LPS) has been considered the primary agent to establish animal models of inflammation, immunological stress, and organ injury.
36587880	11	0	theme	factor	1715:1720	arg1	production					1663:1672	the production	1659:1672	the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α)	1659:1730	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	1	theme	LPS-stimulated	1599:1612	arg1	RAW264.7 cells					1614:1627	LPS-stimulated RAW264.7 cells	1599:1627	LPS-stimulated RAW264.7 cells	1599:1627	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	2	theme	NF-κB	1916:1920	arg1	inhibitor					1889:1897	alpha inhibitor	1883:1897	alpha inhibitor of NF-κB (IκBα)	1883:1913	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	2	theme	NF-κB	1916:1920	arg1	p65					1922:1924	NF-κB p65	1916:1924	NF-κB p65	1916:1924	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	8	3	theme	inflammatory	1255:1266	arg1	IL-6					1283:1286	IL-6	1283:1286	IL-6	1283:1286	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	8	3	theme	inflammatory	1255:1266	arg1	NO					1279:1280	NO	1279:1280	NO	1279:1280	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	8	3	theme	inflammatory	1255:1266	arg1	mediators					1268:1276	inflammatory mediators	1255:1276	inflammatory mediators (NO, IL-6 and TNF-α)	1255:1297	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	8	3	theme	inflammatory	1255:1266	arg1	TNF-α					1292:1296	TNF-α	1292:1296	TNF-α	1292:1296	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	12	4	from	effects	2052:2058	arg1	RAW264.7 cells					2118:2131	LPS-stimulated RAW264.7 cells	2103:2131	LPS-stimulated RAW264.7 cells	2103:2131	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	12	4	from	effects	2052:2058	arg1	production					2067:2076	the production	2063:2076	the production of NO, IL-6 and TNF-α	2063:2098	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	11	5	dep	decreased	1649:1657	arg1	down-regulated					1773:1786	down-regulated	1773:1786	down-regulated the expression of TLR4 and myeloid differentiation factor 88	1773:1847	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	5	dep	decreased	1649:1657	arg1	inhibited					1850:1858	inhibited	1850:1858	inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα	1850:1949	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	3	6	theme	original	537:544	arg1	interest					546:553	the original interest	533:553	the original interest of this study	533:567	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	12	7	theme	inhibitory	2041:2050	arg1	effects					2052:2058	different inhibitory effects	2031:2058	different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells	2031:2131	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	6	8	theme	RAW264.7	961:968	arg1	macrophages					970:980	LPS-stimulated RAW264.7 macrophages	946:980	LPS-stimulated RAW264.7 macrophages model	946:986	Its anti-inflammatory mechanism was explored with LPS-stimulated RAW264.7 macrophages model.
36587880	2	9	theme	previous	298:305	arg1	study					307:311	Our previous study	294:311	Our previous study	294:311	Our previous study found that the dichloromethane extract (DCME) of C. longissima showed anti-inflammatory activity in vitro.
36587880	13	10	theme	promising	2474:2482	arg1	agent					2484:2488	a promising agent	2472:2488	a promising agent for the treatment of inflammatory diseases	2472:2531	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	4	11	theme	accurate	703:710	arg1	components					721:730	the accurate chemical components	699:730	the accurate chemical components responsible for this active extract	699:766	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	1	12	theme	Yao	228:230	arg1	ethnomedicine					232:244	a typical Yao ethnomedicine	218:244	a typical Yao ethnomedicine that has been used to treat arthritis in China	218:291	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	1	12	theme	Yao	228:230	arg1	longissima					204:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	0	13	theme	TLR4/NF-κB	132:141	arg1	pathway					153:159	the TLR4/NF-κB signaling pathway	128:159	the TLR4/NF-κB signaling pathway	128:159	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	11	14	theme	inhibitor	1889:1897	arg1	phosphorylation					1864:1878	the phosphorylation	1860:1878	the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65,	1860:1925	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	14	theme	inhibitor	1889:1897	arg1	degradation					1931:1941	degradation	1931:1941	degradation of IκBα	1931:1949	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	13	15	theme	inflammatory	2511:2522	arg1	diseases					2524:2531	inflammatory diseases	2511:2531	inflammatory diseases	2511:2531	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	8	16	theme	RAW264.7	1302:1309	arg1	macrophages					1311:1321	RAW264.7 macrophages	1302:1321	RAW264.7 macrophages model	1302:1327	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	4	17	theme	active	753:758	arg1	extract					760:766	this active extract	748:766	this active extract	748:766	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	7	18	theme	resonance	1129:1137	arg1	spectroscopy					1139:1150	nuclear magnetic resonance spectroscopy	1112:1150	nuclear magnetic resonance spectroscopy	1112:1150	The compounds in DCME were isolated by repeated column chromatography and their structures were identified on the basis of nuclear magnetic resonance spectroscopy.
36587880	5	19	dep	MATERIALS	769:777	arg1	activity					813:820	The anti-inflammatory activity	791:820	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo	769:836	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo was tested with carrageenan-induced mice paw edema model.
36587880	12	20	theme	LPS-stimulated	2103:2116	arg1	RAW264.7 cells					2118:2131	LPS-stimulated RAW264.7 cells	2103:2131	LPS-stimulated RAW264.7 cells	2103:2131	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	11	21	theme	TLR4	1806:1809	arg1	expression					1792:1801	the expression	1788:1801	the expression of TLR4 and myeloid differentiation factor 88	1788:1847	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	7	22	theme	nuclear	1112:1118	arg1	resonance					1129:1137	nuclear magnetic resonance	1112:1137	nuclear magnetic resonance spectroscopy	1112:1150	The compounds in DCME were isolated by repeated column chromatography and their structures were identified on the basis of nuclear magnetic resonance spectroscopy.
36587880	12	23	theme	NO	2081:2082	arg1	production					2067:2076	the production	2063:2076	the production of NO, IL-6 and TNF-α	2063:2098	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	0	24	theme	diterpenoid	61:71	arg1	phenols					73:79	diterpenoid phenols	61:79	diterpenoid phenols	61:79	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	9	25	theme	docking	1357:1363	arg1	analysis					1365:1372	the molecular docking analysis	1343:1372	the molecular docking analysis	1343:1372	In addition, the molecular docking analysis, which evaluated the potential interaction between the compounds and Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB), was performed.
36587880	10	26	dep	DCME	1525:1528	arg1	alleviated					1542:1551	alleviated	1542:1551	DCME effectively alleviated the mice paw edema induced by carrageenan	1525:1593	RESULTS DCME effectively alleviated the mice paw edema induced by carrageenan.
36587880	1	27	theme	RELEVANCE	183:191	arg1	ethnomedicine					232:244	a typical Yao ethnomedicine	218:244	a typical Yao ethnomedicine that has been used to treat arthritis in China	218:291	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	1	27	theme	RELEVANCE	183:191	arg1	longissima					204:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	12	28	theme	IL-6	2085:2088	arg1	production					2067:2076	the production	2063:2076	the production of NO, IL-6 and TNF-α	2063:2098	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	11	29	theme	factor	1839:1844	arg1	expression					1792:1801	the expression	1788:1801	the expression of TLR4 and myeloid differentiation factor 88	1788:1847	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	5	30	theme	DCME	825:828	arg1	activity					813:820	The anti-inflammatory activity	791:820	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo	769:836	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo was tested with carrageenan-induced mice paw edema model.
36587880	7	31	theme	column	1037:1042	arg1	chromatography					1044:1057	repeated column chromatography	1028:1057	repeated column chromatography	1028:1057	The compounds in DCME were isolated by repeated column chromatography and their structures were identified on the basis of nuclear magnetic resonance spectroscopy.
36587880	11	32	theme	myeloid	1815:1821	arg1	factor					1839:1844	myeloid differentiation factor 88	1815:1847	myeloid differentiation factor 88	1815:1847	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	12	33	theme	diterpenoid	1959:1969	arg1	phenols					1971:1977	Twelve diterpenoid phenols	1952:1977	Twelve diterpenoid phenols	1952:1977	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	0	34	from	rich	53:56	arg1	phenols					73:79	diterpenoid phenols	61:79	diterpenoid phenols	61:79	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	13	35	from	C. longissima	2244:2256	arg1	DCME					2234:2237	DCME	2234:2237	DCME from C. longissima	2234:2256	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	9	36	theme	potential	1395:1403	arg1	interaction					1405:1415	the potential interaction	1391:1415	the potential interaction between the compounds	1391:1437	In addition, the molecular docking analysis, which evaluated the potential interaction between the compounds and Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB), was performed.
36587880	3	37	dep	mechanism	451:459	arg1	the					429:431	the	429:431	the	429:431	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	3	38	theme	detailed	465:472	arg1	composition					483:493	detailed chemical composition	465:493	detailed chemical composition	465:493	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	5	39	theme	carrageenan-induced	854:872	arg1	edema					883:887	carrageenan-induced mice paw edema	854:887	carrageenan-induced mice paw edema model	854:893	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo was tested with carrageenan-induced mice paw edema model.
36587880	13	40	theme	TLR4/NF-κB	2429:2438	arg1	pathway					2450:2456	the TLR4/NF-κB signaling pathway	2425:2456	the TLR4/NF-κB signaling pathway	2425:2456	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	10	41	theme	paw	1562:1564	arg1	edema					1566:1570	the mice paw edema	1553:1570	the mice paw edema induced by carrageenan	1553:1593	RESULTS DCME effectively alleviated the mice paw edema induced by carrageenan.
36587880	13	42	dep	inhibit	2264:2270	arg1	attributed					2337:2346	attributed	2337:2346	is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway	2327:2456	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	5	43	theme	paw	879:881	arg1	edema					883:887	carrageenan-induced mice paw edema	854:887	carrageenan-induced mice paw edema model	854:893	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo was tested with carrageenan-induced mice paw edema model.
36587880	12	44	theme	TNF-α	2094:2098	arg1	production					2067:2076	the production	2063:2076	the production of NO, IL-6 and TNF-α	2063:2098	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	11	45	theme	mRNA	1753:1756	arg1	transcription					1758:1770	their mRNA transcription	1747:1770	their mRNA transcription	1747:1770	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	12	46	theme	molecular	2187:2195	arg1	docking					2197:2203	molecular docking	2187:2203	molecular docking	2187:2203	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	3	47	theme	DCME	498:501	arg1	composition					483:493	detailed chemical composition	465:493	detailed chemical composition	465:493	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	3	47	theme	DCME	498:501	arg1	mechanism					451:459	anti-inflammatory mechanism	433:459	anti-inflammatory mechanism	433:459	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	0	48	theme	Callicarpa	31:40	arg1	longissima					42:51	Callicarpa longissima	31:51	Callicarpa longissima rich in diterpenoid phenols	31:79	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	9	49	theme	Toll-like	1443:1451	arg1	receptor					1453:1460	Toll-like receptor 4	1443:1462	Toll-like receptor 4 (TLR4)	1443:1469	In addition, the molecular docking analysis, which evaluated the potential interaction between the compounds and Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB), was performed.
36587880	9	49	theme	Toll-like	1443:1451	arg1	TLR4					1465:1468	TLR4	1465:1468	TLR4	1465:1468	In addition, the molecular docking analysis, which evaluated the potential interaction between the compounds and Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB), was performed.
36587880	13	50	theme	phenols	2398:2404	arg1	effect					2367:2372	the synergistic effect	2351:2372	the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway	2351:2456	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	11	51	theme	interleukin	1677:1687	arg1	IL					1690:1691	interleukin (IL)-6	1677:1694	interleukin (IL)-6	1677:1694	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	2	52	theme	C. longissima	362:374	arg1	DCME					353:356	DCME	353:356	DCME	353:356	Our previous study found that the dichloromethane extract (DCME) of C. longissima showed anti-inflammatory activity in vitro.
36587880	2	52	theme	C. longissima	362:374	arg1	extract					344:350	the dichloromethane extract	324:350	the dichloromethane extract (DCME) of C. longissima	324:374	Our previous study found that the dichloromethane extract (DCME) of C. longissima showed anti-inflammatory activity in vitro.
36587880	0	53	theme	rich	53:56	arg1	longissima					42:51	Callicarpa longissima	31:51	Callicarpa longissima rich in diterpenoid phenols	31:79	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	11	54	theme	NF-κB	1902:1906	arg1	p65					1922:1924	NF-κB p65	1916:1924	NF-κB p65	1916:1924	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	54	theme	NF-κB	1902:1906	arg1	inhibitor					1889:1897	alpha inhibitor	1883:1897	alpha inhibitor of NF-κB (IκBα)	1883:1913	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	13	55	theme	inflammatory	2272:2283	arg1	response					2285:2292	inflammatory response	2272:2292	inflammatory response	2272:2292	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	4	56	theme	anti-inflammatory	619:635	arg1	properties					637:646	the anti-inflammatory properties	615:646	the anti-inflammatory properties of the DCME from C. longissima	615:677	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	13	57	theme	abundant	2377:2384	arg1	phenols					2398:2404	abundant diterpenoid phenols	2377:2404	abundant diterpenoid phenols	2377:2404	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	4	58	from	properties	637:646	arg1	C. longissima					665:677	C. longissima	665:677	C. longissima	665:677	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	11	59	theme	IL	1690:1691	arg1	production					1663:1672	the production	1659:1672	the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α)	1659:1730	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	2	60	theme	anti-inflammatory	383:399	arg1	activity					401:408	anti-inflammatory activity	383:408	anti-inflammatory activity	383:408	Our previous study found that the dichloromethane extract (DCME) of C. longissima showed anti-inflammatory activity in vitro.
36587880	11	61	theme	tumor	1700:1704	arg1	factor					1715:1720	tumor necrosis factor α	1700:1722	tumor necrosis factor α (TNF-α)	1700:1730	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	61	theme	tumor	1700:1704	arg1	TNF-α					1725:1729	TNF-α	1725:1729	TNF-α	1725:1729	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	6	62	theme	macrophages	970:980	arg1	model					982:986	LPS-stimulated RAW264.7 macrophages model	946:986	LPS-stimulated RAW264.7 macrophages model	946:986	Its anti-inflammatory mechanism was explored with LPS-stimulated RAW264.7 macrophages model.
36587880	13	63	theme	diseases	2524:2531	arg1	treatment					2498:2506	the treatment	2494:2506	the treatment of inflammatory diseases	2494:2531	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	3	64	theme	study	563:567	arg1	interest					546:553	the original interest	533:553	the original interest of this study	533:567	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	12	65	theme	different	2031:2039	arg1	effects					2052:2058	different inhibitory effects	2031:2058	different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells	2031:2131	Twelve diterpenoid phenols were identified from DCME, and they not only showed different inhibitory effects on the production of NO, IL-6 and TNF-α in LPS-stimulated RAW264.7 cells, but also could bind to TLR4 and NF-κB as analyzed by molecular docking.
36587880	6	66	theme	LPS-stimulated	946:959	arg1	macrophages					970:980	LPS-stimulated RAW264.7 macrophages	946:980	LPS-stimulated RAW264.7 macrophages model	946:986	Its anti-inflammatory mechanism was explored with LPS-stimulated RAW264.7 macrophages model.
36587880	8	67	theme	macrophages	1311:1321	arg1	model					1323:1327	RAW264.7 macrophages model	1302:1327	RAW264.7 macrophages model	1302:1327	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	1	68	theme	typical	220:226	arg1	ethnomedicine					232:244	a typical Yao ethnomedicine	218:244	a typical Yao ethnomedicine that has been used to treat arthritis in China	218:291	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	1	68	theme	typical	220:226	arg1	longissima					204:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	0	69	theme	signaling	143:151	arg1	pathway					153:159	the TLR4/NF-κB signaling pathway	128:159	the TLR4/NF-κB signaling pathway	128:159	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	2	70	theme	dichloromethane	328:342	arg1	DCME					353:356	DCME	353:356	DCME	353:356	Our previous study found that the dichloromethane extract (DCME) of C. longissima showed anti-inflammatory activity in vitro.
36587880	2	70	theme	dichloromethane	328:342	arg1	extract					344:350	the dichloromethane extract	324:350	the dichloromethane extract (DCME) of C. longissima	324:374	Our previous study found that the dichloromethane extract (DCME) of C. longissima showed anti-inflammatory activity in vitro.
36587880	4	71	from	C. longissima	665:677	arg1	properties					637:646	the anti-inflammatory properties	615:646	the anti-inflammatory properties of the DCME from C. longissima	615:677	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	4	71	from	C. longissima	665:677	arg1	DCME					655:658	the DCME	651:658	the DCME from C. longissima	651:677	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	5	72	theme	edema	883:887	arg1	model					889:893	carrageenan-induced mice paw edema model	854:893	carrageenan-induced mice paw edema model	854:893	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo was tested with carrageenan-induced mice paw edema model.
36587880	4	73	theme	DCME	655:658	arg1	properties					637:646	the anti-inflammatory properties	615:646	the anti-inflammatory properties of the DCME from C. longissima	615:677	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	7	74	theme	spectroscopy	1139:1150	arg1	basis					1103:1107	the basis	1099:1107	the basis of nuclear magnetic resonance spectroscopy	1099:1150	The compounds in DCME were isolated by repeated column chromatography and their structures were identified on the basis of nuclear magnetic resonance spectroscopy.
36587880	0	75	from	phenols	73:79	arg1	rich					53:56	rich	53:56	rich	53:56	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	7	76	theme	magnetic	1120:1127	arg1	resonance					1129:1137	nuclear magnetic resonance	1112:1137	nuclear magnetic resonance spectroscopy	1112:1150	The compounds in DCME were isolated by repeated column chromatography and their structures were identified on the basis of nuclear magnetic resonance spectroscopy.
36587880	4	77	dep	aimed	597:601	arg1	AIM					570:572	AIM	570:572	AIM OF THE STUDY	570:585	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	11	78	theme	alpha	1883:1887	arg1	p65					1922:1924	NF-κB p65	1916:1924	NF-κB p65	1916:1924	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	78	theme	alpha	1883:1887	arg1	inhibitor					1889:1897	alpha inhibitor	1883:1897	alpha inhibitor of NF-κB (IκBα)	1883:1913	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	5	79	theme	anti-inflammatory	795:811	arg1	activity					813:820	The anti-inflammatory activity	791:820	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo	769:836	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo was tested with carrageenan-induced mice paw edema model.
36587880	9	80	theme	molecular	1347:1355	arg1	analysis					1365:1372	the molecular docking analysis	1343:1372	the molecular docking analysis	1343:1372	In addition, the molecular docking analysis, which evaluated the potential interaction between the compounds and Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB), was performed.
36587880	1	81	theme	ETHNOPHARMACOLOGICAL	162:181	arg1	ethnomedicine					232:244	a typical Yao ethnomedicine	218:244	a typical Yao ethnomedicine that has been used to treat arthritis in China	218:291	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	1	81	theme	ETHNOPHARMACOLOGICAL	162:181	arg1	longissima					204:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	4	82	theme	chemical	712:719	arg1	components					721:730	the accurate chemical components	699:730	the accurate chemical components responsible for this active extract	699:766	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	0	83	theme	anti-inflammatory	88:104	arg1	effect					106:111	anti-inflammatory effect	88:111	anti-inflammatory effect	88:111	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	1	84	theme	Callicarpa	193:202	arg1	ethnomedicine					232:244	a typical Yao ethnomedicine	218:244	a typical Yao ethnomedicine that has been used to treat arthritis in China	218:291	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	1	84	theme	Callicarpa	193:202	arg1	longissima					204:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	8	85	dep	mediators	1268:1276	arg1	IL-6					1283:1286	IL-6	1283:1286	IL-6	1283:1286	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	8	85	dep	mediators	1268:1276	arg1	NO					1279:1280	NO	1279:1280	NO	1279:1280	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	8	85	dep	mediators	1268:1276	arg1	mediators					1268:1276	inflammatory mediators	1255:1276	inflammatory mediators (NO, IL-6 and TNF-α)	1255:1297	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	8	85	dep	mediators	1268:1276	arg1	TNF-α					1292:1296	TNF-α	1292:1296	TNF-α	1292:1296	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	4	86	theme	responsible	732:742	arg1	components					721:730	the accurate chemical components	699:730	the accurate chemical components responsible for this active extract	699:766	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	11	87	theme	differentiation	1823:1837	arg1	factor					1839:1844	myeloid differentiation factor 88	1815:1847	myeloid differentiation factor 88	1815:1847	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	7	88	theme	repeated	1028:1035	arg1	chromatography					1044:1057	repeated column chromatography	1028:1057	repeated column chromatography	1028:1057	The compounds in DCME were isolated by repeated column chromatography and their structures were identified on the basis of nuclear magnetic resonance spectroscopy.
36587880	11	89	theme	IκBα	1946:1949	arg1	phosphorylation					1864:1878	the phosphorylation	1860:1878	the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65,	1860:1925	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	89	theme	IκBα	1946:1949	arg1	degradation					1931:1941	degradation	1931:1941	degradation of IκBα	1931:1949	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	0	90	theme	dichloromethane	4:18	arg1	extract					20:26	The dichloromethane extract	0:26	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols	0:79	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	8	91	theme	anti-inflammatory	1157:1173	arg1	activities					1175:1184	The anti-inflammatory activities	1153:1184	The anti-inflammatory activities of the isolates in vitro	1153:1209	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	4	92	theme	STUDY	581:585	arg1	AIM					570:572	AIM	570:572	AIM OF THE STUDY	570:585	AIM OF THE STUDY The study aimed to evaluate the anti-inflammatory properties of the DCME from C. longissima and further explore the accurate chemical components responsible for this active extract.
36587880	3	93	theme	chemical	474:481	arg1	composition					483:493	detailed chemical composition	465:493	detailed chemical composition	465:493	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	5	94	theme	mice	874:877	arg1	edema					883:887	carrageenan-induced mice paw edema	854:887	carrageenan-induced mice paw edema model	854:893	MATERIALS AND METHODS The anti-inflammatory activity of DCME in vivo was tested with carrageenan-induced mice paw edema model.
36587880	13	95	theme	signaling	2440:2448	arg1	pathway					2450:2456	the TLR4/NF-κB signaling pathway	2425:2456	the TLR4/NF-κB signaling pathway	2425:2456	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	10	96	theme	mice	1557:1560	arg1	edema					1566:1570	the mice paw edema	1553:1570	the mice paw edema induced by carrageenan	1553:1593	RESULTS DCME effectively alleviated the mice paw edema induced by carrageenan.
36587880	7	97	from	compounds	993:1001	arg1	DCME					1006:1009	DCME	1006:1009	DCME	1006:1009	The compounds in DCME were isolated by repeated column chromatography and their structures were identified on the basis of nuclear magnetic resonance spectroscopy.
36587880	0	98	theme	longissima	42:51	arg1	extract					20:26	The dichloromethane extract	0:26	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols	0:79	The dichloromethane extract of Callicarpa longissima rich in diterpenoid phenols exerts anti-inflammatory effect via inhibiting the TLR4/NF-κB signaling pathway.
36587880	8	99	theme	isolates	1193:1200	arg1	activities					1175:1184	The anti-inflammatory activities	1153:1184	The anti-inflammatory activities of the isolates in vitro	1153:1209	The anti-inflammatory activities of the isolates in vitro were also tested by suppressing releases of inflammatory mediators (NO, IL-6 and TNF-α) in RAW264.7 macrophages model.
36587880	13	100	theme	diterpenoid	2386:2396	arg1	phenols					2398:2404	abundant diterpenoid phenols	2377:2404	abundant diterpenoid phenols	2377:2404	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	6	101	theme	anti-inflammatory	900:916	arg1	mechanism					918:926	Its anti-inflammatory mechanism	896:926	Its anti-inflammatory mechanism	896:926	Its anti-inflammatory mechanism was explored with LPS-stimulated RAW264.7 macrophages model.
36587880	3	102	theme	anti-inflammatory	433:449	arg1	mechanism					451:459	anti-inflammatory mechanism	433:459	anti-inflammatory mechanism	433:459	However, the anti-inflammatory mechanism and detailed chemical composition of DCME remain unclear, which lead to the original interest of this study.
36587880	13	103	theme	synergistic	2355:2365	arg1	effect					2367:2372	the synergistic effect	2351:2372	the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway	2351:2456	CONCLUSIONS Taken together, DCME from C. longissima could inhibit inflammatory response both in vitro and in vivo, which is mainly attributed to the synergistic effect of abundant diterpenoid phenols through inhibiting the TLR4/NF-κB signaling pathway, and might be a promising agent for the treatment of inflammatory diseases.
36587880	1	104	used	used	260:263	arg2	longissima					204:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima	162:213	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	1	104	used	used	260:263	arg2	ethnomedicine					232:244	a typical Yao ethnomedicine	218:244	a typical Yao ethnomedicine that has been used to treat arthritis in China	218:291	ETHNOPHARMACOLOGICAL RELEVANCE Callicarpa longissima is a typical Yao ethnomedicine that has been used to treat arthritis in China.
36587880	11	105	theme	necrosis	1706:1713	arg1	factor					1715:1720	tumor necrosis factor α	1700:1722	tumor necrosis factor α (TNF-α)	1700:1730	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	11	105	theme	necrosis	1706:1713	arg1	TNF-α					1725:1729	TNF-α	1725:1729	TNF-α	1725:1729	In LPS-stimulated RAW264.7 cells, DCME significantly decreased the production of interleukin (IL)-6 and tumor necrosis factor α (TNF-α) via inhibiting their mRNA transcription, down-regulated the expression of TLR4 and myeloid differentiation factor 88, inhibited the phosphorylation of alpha inhibitor of NF-κB (IκBα), NF-κB p65, and degradation of IκBα.
36587880	9	106	theme	nuclear	1475:1481	arg1	factor					1483:1488	nuclear factor κB	1475:1491	nuclear factor κB (NF-κB)	1475:1499	In addition, the molecular docking analysis, which evaluated the potential interaction between the compounds and Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB), was performed.
36587880	9	106	theme	nuclear	1475:1481	arg1	NF-κB					1494:1498	NF-κB	1494:1498	NF-κB	1494:1498	In addition, the molecular docking analysis, which evaluated the potential interaction between the compounds and Toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB), was performed.
35477942	7	0	theme	intratumor	1118:1127	arg1	injection					1129:1137	intratumor injection	1118:1137	intratumor injection	1118:1137	After intratumor injection, this injectable hydrogel efficiently inhibites the tumor growth under 808 nm laser irradiation.
35477942	2	1	theme	injectable	359:368	arg1	hydrogel					386:393	an injectable thermosensitive hydrogel	356:393	an injectable thermosensitive hydrogel based on iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI)	356:460	In this work, an injectable thermosensitive hydrogel based on iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI) is developed to overcome this problem.
35477942	6	2	theme	excellent	999:1007	arg1	stability					1017:1025	excellent thermal stability	999:1025	excellent thermal stability	999:1025	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	0	3	theme	Photothermal	98:109	arg1	Therapy					111:117	Cancer Photothermal Therapy	91:117	Cancer Photothermal Therapy	91:117	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	9	4	theme	preparation	1509:1519	arg1	advantages					1467:1476	advantages	1467:1476	advantages of good biocompatible and easy preparation	1467:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	9	5	theme	iodine-starch	1428:1440	arg1	hydrogel					1453:1460	this injectable thermosensitive "iodine-starch" composite hydrogel	1395:1460	this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation	1395:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	6	6	theme	iodine-starch	1088:1100	arg1	complex					1103:1109	the "iodine-starch" complex	1083:1109	the "iodine-starch" complex	1083:1109	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	6	7	theme	laser	909:913	arg1	irradiation					915:925	NIR laser irradiation	905:925	NIR laser irradiation for 10 min	905:936	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	9	8	theme	"	1441:1441	arg1	hydrogel					1453:1460	this injectable thermosensitive "iodine-starch" composite hydrogel	1395:1460	this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation	1395:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	7	9	theme	laser	1217:1221	arg1	irradiation					1223:1233	808 nm laser irradiation	1210:1233	808 nm laser irradiation	1210:1233	After intratumor injection, this injectable hydrogel efficiently inhibites the tumor growth under 808 nm laser irradiation.
35477942	6	10	theme	NIR	905:907	arg1	irradiation					915:925	NIR laser irradiation	905:925	NIR laser irradiation for 10 min	905:936	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	4	11	dep	show	699:702	arg1	self-assembles					717:730	self-assembles	717:730	show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm	699:783	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	4	12	theme	PNSI	704:707	arg1	polymer					709:715	PNSI polymer	704:715	PNSI polymer	704:715	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	9	13	theme	composite	1443:1451	arg1	hydrogel					1453:1460	this injectable thermosensitive "iodine-starch" composite hydrogel	1395:1460	this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation	1395:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	8	14	theme	wound	1309:1313	arg1	post-PTT					1315:1322	the wound post-PTT	1305:1322	the wound post-PTT due to the release of iodine, which promotes wound healing	1305:1381	Furthermore, it can also suppress Staphylococcus aureus infection in the wound post-PTT due to the release of iodine, which promotes wound healing.
35477942	3	15	theme	UV-vis	520:525	arg1	spectra					527:533	UV-vis spectra	520:533	UV-vis spectra	520:533	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	9	16	theme	good	1481:1484	arg1	preparation					1509:1519	good biocompatible and easy preparation	1481:1519	good biocompatible and easy preparation	1481:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	7	17	theme	injectable	1145:1154	arg1	hydrogel					1156:1163	this injectable hydrogel	1140:1163	this injectable hydrogel	1140:1163	After intratumor injection, this injectable hydrogel efficiently inhibites the tumor growth under 808 nm laser irradiation.
35477942	7	18	theme	808 nm	1210:1215	arg1	irradiation					1223:1233	808 nm laser irradiation	1210:1233	808 nm laser irradiation	1210:1233	After intratumor injection, this injectable hydrogel efficiently inhibites the tumor growth under 808 nm laser irradiation.
35477942	3	19	with	copolymerization	553:568	arg1	starch					606:611	starch	606:611	starch	606:611	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	3	19	with	copolymerization	553:568	arg1	formation					621:629	the formation	617:629	the formation of "iodine-starch" complex	617:656	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	2	20	theme	iodine-loaded	404:416	arg1	N-isopropylacrylamide					432:452	N-isopropylacrylamide	432:452	N-isopropylacrylamide	432:452	In this work, an injectable thermosensitive hydrogel based on iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI) is developed to overcome this problem.
35477942	2	20	theme	iodine-loaded	404:416	arg1	PNSI					456:459	PNSI	456:459	PNSI	456:459	In this work, an injectable thermosensitive hydrogel based on iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI) is developed to overcome this problem.
35477942	2	20	theme	iodine-loaded	404:416	arg1	starch-g-poly					418:430	iodine-loaded starch-g-poly	404:430	iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI)	404:460	In this work, an injectable thermosensitive hydrogel based on iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI) is developed to overcome this problem.
35477942	0	21	theme	Thermosensitive	11:25	arg1	N-isopropylacrylamide					55:75	N-isopropylacrylamide	55:75	N-isopropylacrylamide	55:75	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	0	21	theme	Thermosensitive	11:25	arg1	Starch-g-poly					41:53	Thermosensitive Iodine-Loaded Starch-g-poly	11:53	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy	0:117	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	4	22	theme	electron	672:679	arg1	images					692:697	Transmission electron microscope images	659:697	Transmission electron microscope images	659:697	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	0	23	theme	Injectable	0:9	arg1	Hydrogel					78:85	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel	0:85	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy	0:117	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	3	24	theme	poly	573:576	arg1	copolymerization					553:568	the graft copolymerization	543:568	the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex	543:656	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	4	25	theme	Transmission	659:670	arg1	images					692:697	Transmission electron microscope images	659:697	Transmission electron microscope images	659:697	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	0	26	theme	Starch-g-poly	41:53	arg1	Hydrogel					78:85	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel	0:85	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy	0:117	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	4	27	theme	spherical	745:753	arg1	nanogel					755:761	regular spherical nanogel	737:761	regular spherical nanogel with a size of ≈50 nm	737:783	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	3	28	theme	graft	547:551	arg1	copolymerization					553:568	the graft copolymerization	543:568	the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex	543:656	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	5	29	theme	room	834:837	arg1	temperature					839:849	room temperature	834:849	room temperature	834:849	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	0	30	theme	Iodine-Loaded	27:39	arg1	N-isopropylacrylamide					55:75	N-isopropylacrylamide	55:75	N-isopropylacrylamide	55:75	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	0	30	theme	Iodine-Loaded	27:39	arg1	Starch-g-poly					41:53	Thermosensitive Iodine-Loaded Starch-g-poly	11:53	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy	0:117	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	6	31	theme	nanogel	953:959	arg1	dispersion					961:970	the nanogel dispersion	949:970	the nanogel dispersion	949:970	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	9	32	theme	potential	1531:1539	arg1	application					1541:1551	potential application	1531:1551	potential application for tumor photothermal therapy and antibacterial infection	1531:1610	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	3	33	theme	iodine-starch	635:647	arg1	complex					650:656	"iodine-starch" complex	634:656	"iodine-starch" complex	634:656	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	9	34	theme	biocompatible	1486:1498	arg1	preparation					1509:1519	good biocompatible and easy preparation	1481:1519	good biocompatible and easy preparation	1481:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	3	35	theme	H	509:509	arg1	NMR					511:513	1 H NMR	507:513	1 H NMR	507:513	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	9	36	contain	possesses	1521:1529	arg1	hydrogel					1453:1460	this injectable thermosensitive "iodine-starch" composite hydrogel	1395:1460	this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation	1395:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	9	36	contain	possesses	1521:1529	arg2	application					1541:1551	potential application	1531:1551	potential application for tumor photothermal therapy and antibacterial infection	1531:1610	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	3	37	theme	"	648:648	arg1	complex					650:656	"iodine-starch" complex	634:656	"iodine-starch" complex	634:656	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	1	38	theme	normal	213:218	arg1	tissues					220:226	the normal tissues	209:226	the normal tissues near tumors	209:238	Although photothermal therapy (PTT) can effectively eliminate tumors, the normal tissues near tumors are inevitably damaged by heat and infected by bacteria, which greatly limits the therapeutic effect.
35477942	9	39	theme	photothermal	1563:1574	arg1	therapy					1576:1582	tumor photothermal therapy	1557:1582	tumor photothermal therapy	1557:1582	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	5	40	theme	concentrated	790:801	arg1	dispersion					811:820	The concentrated nanogel dispersion	786:820	The concentrated nanogel dispersion	786:820	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	5	40	theme	concentrated	790:801	arg1	sol					827:829	a sol	825:829	a sol at room temperature	825:849	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	9	41	theme	injectable	1400:1409	arg1	hydrogel					1453:1460	this injectable thermosensitive "iodine-starch" composite hydrogel	1395:1460	this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation	1395:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	9	42	theme	easy	1504:1507	arg1	preparation					1509:1519	good biocompatible and easy preparation	1481:1519	good biocompatible and easy preparation	1481:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	5	43	theme	nanogel	803:809	arg1	dispersion					811:820	The concentrated nanogel dispersion	786:820	The concentrated nanogel dispersion	786:820	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	5	43	theme	nanogel	803:809	arg1	sol					827:829	a sol	825:829	a sol at room temperature	825:849	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	1	44	theme	therapeutic	322:332	arg1	effect					334:339	the therapeutic effect	318:339	the therapeutic effect	318:339	Although photothermal therapy (PTT) can effectively eliminate tumors, the normal tissues near tumors are inevitably damaged by heat and infected by bacteria, which greatly limits the therapeutic effect.
35477942	9	45	theme	tumor	1557:1561	arg1	therapy					1576:1582	tumor photothermal therapy	1557:1582	tumor photothermal therapy	1557:1582	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	6	46	theme	dispersion	961:970	arg1	ΔT					943:944	the ΔT	939:944	the ΔT of the nanogel dispersion	939:970	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	8	47	theme	wound	1369:1373	arg1	healing					1375:1381	wound healing	1369:1381	wound healing	1369:1381	Furthermore, it can also suppress Staphylococcus aureus infection in the wound post-PTT due to the release of iodine, which promotes wound healing.
35477942	1	48	theme	photothermal	148:159	arg1	PTT					170:172	PTT	170:172	PTT	170:172	Although photothermal therapy (PTT) can effectively eliminate tumors, the normal tissues near tumors are inevitably damaged by heat and infected by bacteria, which greatly limits the therapeutic effect.
35477942	1	48	theme	photothermal	148:159	arg1	therapy					161:167	photothermal therapy	148:167	photothermal therapy (PTT)	148:173	Although photothermal therapy (PTT) can effectively eliminate tumors, the normal tissues near tumors are inevitably damaged by heat and infected by bacteria, which greatly limits the therapeutic effect.
35477942	4	49	with	nanogel	755:761	arg1	size					770:773	a size	768:773	a size of ≈50 nm	768:783	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	9	50	with	hydrogel	1453:1460	arg1	advantages					1467:1476	advantages	1467:1476	advantages of good biocompatible and easy preparation	1467:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	5	51	from	temperature	839:849	arg1	dispersion					811:820	The concentrated nanogel dispersion	786:820	The concentrated nanogel dispersion	786:820	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	5	51	from	temperature	839:849	arg1	sol					827:829	a sol	825:829	a sol at room temperature	825:849	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	4	52	theme	regular	737:743	arg1	nanogel					755:761	regular spherical nanogel	737:761	regular spherical nanogel with a size of ≈50 nm	737:783	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	5	53	theme	body	881:884	arg1	temperature					886:896	body temperature	881:896	body temperature	881:896	The concentrated nanogel dispersion is a sol at room temperature and transforms to hydrogel at body temperature.
35477942	6	54	theme	high	1031:1034	arg1	cytotoxicity					1036:1047	high cytotoxicity	1031:1047	high cytotoxicity	1031:1047	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	4	55	theme	≈50 nm	778:783	arg1	size					770:773	a size	768:773	a size of ≈50 nm	768:783	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	3	56	theme	complex	650:656	arg1	starch					606:611	starch	606:611	starch	606:611	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	3	56	theme	complex	650:656	arg1	formation					621:629	the formation	617:629	the formation of "iodine-starch" complex	617:656	FTIR, 1 H NMR, and UV-vis spectra confirm the graft copolymerization of poly(N-isopropylacrylamide) with starch and the formation of "iodine-starch" complex.
35477942	6	57	theme	photothermal	1060:1071	arg1	effect					1073:1078	the photothermal effect	1056:1078	the photothermal effect of the "iodine-starch" complex	1056:1109	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	4	58	theme	microscope	681:690	arg1	images					692:697	Transmission electron microscope images	659:697	Transmission electron microscope images	659:697	Transmission electron microscope images show PNSI polymer self-assembles into regular spherical nanogel with a size of ≈50 nm.
35477942	8	59	theme	iodine	1346:1351	arg1	release					1335:1341	the release	1331:1341	the release	1331:1341	Furthermore, it can also suppress Staphylococcus aureus infection in the wound post-PTT due to the release of iodine, which promotes wound healing.
35477942	8	60	theme	due	1324:1326	arg1	post-PTT					1315:1322	the wound post-PTT	1305:1322	the wound post-PTT due to the release of iodine, which promotes wound healing	1305:1381	Furthermore, it can also suppress Staphylococcus aureus infection in the wound post-PTT due to the release of iodine, which promotes wound healing.
35477942	0	61	theme	Cancer	91:96	arg1	Therapy					111:117	Cancer Photothermal Therapy	91:117	Cancer Photothermal Therapy	91:117	Injectable Thermosensitive Iodine-Loaded Starch-g-poly(N-isopropylacrylamide) Hydrogel for Cancer Photothermal Therapy and Anti-Infection.
35477942	6	62	theme	"	1101:1101	arg1	complex					1103:1109	the "iodine-starch" complex	1083:1109	the "iodine-starch" complex	1083:1109	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	8	63	theme	aureus	1285:1290	arg1	infection					1292:1300	Staphylococcus aureus infection	1270:1300	Staphylococcus aureus infection	1270:1300	Furthermore, it can also suppress Staphylococcus aureus infection in the wound post-PTT due to the release of iodine, which promotes wound healing.
35477942	6	64	theme	complex	1103:1109	arg1	effect					1073:1078	the photothermal effect	1056:1078	the photothermal effect of the "iodine-starch" complex	1056:1109	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
35477942	9	65	theme	antibacterial	1588:1600	arg1	infection					1602:1610	antibacterial infection	1588:1610	antibacterial infection	1588:1610	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	9	66	theme	thermosensitive	1411:1425	arg1	hydrogel					1453:1460	this injectable thermosensitive "iodine-starch" composite hydrogel	1395:1460	this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation	1395:1519	Therefore, this injectable thermosensitive "iodine-starch" composite hydrogel with advantages of good biocompatible and easy preparation possesses potential application for tumor photothermal therapy and antibacterial infection.
35477942	2	67	theme	thermosensitive	370:384	arg1	hydrogel					386:393	an injectable thermosensitive hydrogel	356:393	an injectable thermosensitive hydrogel based on iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI)	356:460	In this work, an injectable thermosensitive hydrogel based on iodine-loaded starch-g-poly(N-isopropylacrylamide) (PNSI) is developed to overcome this problem.
35477942	7	68	theme	tumor	1191:1195	arg1	growth					1197:1202	the tumor growth	1187:1202	the tumor growth	1187:1202	After intratumor injection, this injectable hydrogel efficiently inhibites the tumor growth under 808 nm laser irradiation.
35477942	6	69	theme	thermal	1009:1015	arg1	stability					1017:1025	excellent thermal stability	999:1025	excellent thermal stability	999:1025	Under NIR laser irradiation for 10 min, the ΔT of the nanogel dispersion approachs about 20 °C with excellent thermal stability and high cytotoxicity due to the photothermal effect of the "iodine-starch" complex.
37321008	6	0	theme	macrophage	868:877	arg1	polarization					879:890	M2 macrophage polarization	865:890	M2 macrophage polarization	865:890	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	11	1	theme	great	1739:1743	arg1	potential					1745:1753	great potential	1739:1753	great potential	1739:1753	Therefore, the prepared COS@Mn-MSN shows great potential to active scarless wound therapy.
37321008	3	2	theme	Mn-Si-chitooligosaccharides	437:463	arg1	structure					424:432	a composite structure	412:432	a composite structure of Mn-Si-chitooligosaccharides (COS)	412:469	In this work, a composite structure of Mn-Si-chitooligosaccharides (COS) was designed (COS@Mn-MSN) and the ability of regulating wound microenvironment for inhibiting skin fibrosis was investigated.
37321008	5	3	theme	excess	786:791	arg1	ROS					807:809	scavenging excess intracellular ROS	775:809	scavenging excess intracellular ROS	775:809	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	1	4	theme	wound	199:203	arg1	healing					205:211	wound healing	199:211	wound healing	199:211	Excessive oxidative stress and inflammation often impede wound healing and ultimately lead to excessive skin fibrosis formation.
37321008	6	5	theme	COS	844:846	arg1	Mn-MSN					848:853	COS@Mn-MSN	844:853	COS@Mn-MSN	844:853	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	7	6	theme	@	1029:1029	arg1	Mn-MSN					1030:1035	COS@Mn-MSN	1026:1035	COS@Mn-MSN	1026:1035	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	10	7	theme	fibrosis	1640:1647	arg1	formation					1649:1657	excessive skin fibrosis formation	1625:1657	excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal	1625:1695	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	9	8	theme	inflammatory	1345:1356	arg1	microenvironment					1358:1373	the inflammatory microenvironment	1341:1373	the inflammatory microenvironment mediated by COS@Mn-MSN	1341:1396	Besides, the inflammatory microenvironment mediated by COS@Mn-MSN downregulated Smad-7 gene expression and upregulated Col-1α gene expression.
37321008	7	9	attach	released	1012:1019	arg1	Mn-MSN					1030:1035	COS@Mn-MSN	1026:1035	COS@Mn-MSN	1026:1035	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	9	attach	released	1012:1019	arg2	COS					1008:1010	COS	1008:1010	COS released from COS@Mn-MSN	1008:1035	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	4	10	theme	Mn	640:641	arg1	effects					629:635	the negative effects	616:635	the negative effects of Mn	616:641	In order to reduce the negative effects of Mn, the nano-level Mn was doped into MSN to minimize its content.
37321008	8	11	theme	fibroblast	1278:1287	arg1	cells					1289:1293	fibroblast cells	1278:1293	fibroblast cells (L929)	1278:1300	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	8	11	theme	fibroblast	1278:1287	arg1	L929					1296:1299	L929	1296:1299	L929	1296:1299	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	10	12	theme	excessive	1625:1633	arg1	formation					1649:1657	excessive skin fibrosis formation	1625:1657	excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal	1625:1695	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	6	13	attach	released	830:837	arg1	Mn-MSN					848:853	COS@Mn-MSN	844:853	COS@Mn-MSN	844:853	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	6	13	attach	released	830:837	arg2	Si					827:828	The Si	823:828	The Si released from COS@Mn-MSN	823:853	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	7	14	from	expression	1131:1140	arg1	time					1208:1211	the whole time	1198:1211	the whole time	1198:1211	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	5	15	theme	intracellular	793:805	arg1	ROS					807:809	scavenging excess intracellular ROS	775:809	scavenging excess intracellular ROS	775:809	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	1	16	theme	excessive	236:244	arg1	formation					260:268	excessive skin fibrosis formation	236:268	excessive skin fibrosis formation	236:268	Excessive oxidative stress and inflammation often impede wound healing and ultimately lead to excessive skin fibrosis formation.
37321008	6	17	theme	later	899:903	arg1	stage					905:909	the later stage	895:909	the later stage (1-3 d)	895:917	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	6	17	theme	later	899:903	arg1	d					916:916	1-3 d	912:916	1-3 d	912:916	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	10	18	theme	0-1	1523:1525	arg1	stress					1515:1520	oxidative stress	1505:1520	oxidative stress (0-1 d)	1505:1528	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	10	18	theme	0-1	1523:1525	arg1	d					1527:1527	0-1 d	1523:1527	0-1 d	1523:1527	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	9	19	theme	Smad-7	1412:1417	arg1	expression					1424:1433	Smad-7 gene expression	1412:1433	Smad-7 gene expression	1412:1433	Besides, the inflammatory microenvironment mediated by COS@Mn-MSN downregulated Smad-7 gene expression and upregulated Col-1α gene expression.
37321008	8	20	theme	fibrosis	1232:1239	arg1	TGF-β1					1259:1264	fibrosis associated factor TGF-β1	1232:1264	fibrosis associated factor TGF-β1	1232:1264	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	5	21	theme	COS	734:736	arg1	Mn-MSN					738:743	COS@Mn-MSN	734:743	COS@Mn-MSN	734:743	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	8	22	theme	factor	1252:1257	arg1	TGF-β1					1259:1264	fibrosis associated factor TGF-β1	1232:1264	fibrosis associated factor TGF-β1	1232:1264	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	0	23	theme	excessive	79:87	arg1	fibrosis					94:101	excessive skin fibrosis	79:101	excessive skin fibrosis	79:101	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	8	24	from	expression	1218:1227	arg1	cells					1289:1293	fibroblast cells	1278:1293	fibroblast cells (L929)	1278:1300	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	8	24	from	expression	1218:1227	arg1	L929					1296:1299	L929	1296:1299	L929	1296:1299	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	6	25	theme	anti-inflammatory	928:944	arg1	effect					946:951	anti-inflammatory effect	928:951	anti-inflammatory effect	928:951	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	7	26	theme	factors	1162:1168	arg1	expression					1131:1140	expression	1131:1140	expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time	1131:1211	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	27	theme	pro-inflammatory	1145:1160	arg1	CD80					1178:1181	CD80	1178:1181	CD80	1178:1181	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	27	theme	pro-inflammatory	1145:1160	arg1	IL-1β					1188:1192	IL-1β	1188:1192	IL-1β	1188:1192	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	27	theme	pro-inflammatory	1145:1160	arg1	TNF-α					1171:1175	TNF-α	1171:1175	TNF-α	1171:1175	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	27	theme	pro-inflammatory	1145:1160	arg1	factors					1162:1168	pro-inflammatory factors	1145:1168	pro-inflammatory factors (TNF-α, CD80, and IL-1β)	1145:1193	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	9	28	theme	gene	1458:1461	arg1	expression					1463:1472	Col-1α gene expression	1451:1472	Col-1α gene expression	1451:1472	Besides, the inflammatory microenvironment mediated by COS@Mn-MSN downregulated Smad-7 gene expression and upregulated Col-1α gene expression.
37321008	1	29	theme	oxidative	152:160	arg1	stress					162:167	Excessive oxidative stress	142:167	Excessive oxidative stress	142:167	Excessive oxidative stress and inflammation often impede wound healing and ultimately lead to excessive skin fibrosis formation.
37321008	11	30	theme	wound	1774:1778	arg1	therapy					1780:1786	active scarless wound therapy	1758:1786	active scarless wound therapy	1758:1786	Therefore, the prepared COS@Mn-MSN shows great potential to active scarless wound therapy.
37321008	10	31	theme	TGF-β1	1535:1540	arg1	inhibition					1542:1551	the TGF-β1 inhibition	1531:1551	the TGF-β1 inhibition (1-3 d)	1531:1559	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	10	31	theme	TGF-β1	1535:1540	arg1	d					1558:1558	1-3 d	1554:1558	1-3 d	1554:1558	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	0	32	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis	0:101	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	7	33	dep	factors	1162:1168	arg1	CD80					1178:1181	CD80	1178:1181	CD80	1178:1181	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	33	dep	factors	1162:1168	arg1	IL-1β					1188:1192	IL-1β	1188:1192	IL-1β	1188:1192	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	33	dep	factors	1162:1168	arg1	TNF-α					1171:1175	TNF-α	1171:1175	TNF-α	1171:1175	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	33	dep	factors	1162:1168	arg1	factors					1162:1168	pro-inflammatory factors	1145:1168	pro-inflammatory factors (TNF-α, CD80, and IL-1β)	1145:1193	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	5	34	theme	ROS	807:809	arg1	ability					764:770	significant ability	752:770	significant ability of scavenging excess intracellular ROS	752:809	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	3	35	theme	wound	527:531	arg1	microenvironment					533:548	wound microenvironment	527:548	wound microenvironment for inhibiting skin fibrosis	527:577	In this work, a composite structure of Mn-Si-chitooligosaccharides (COS) was designed (COS@Mn-MSN) and the ability of regulating wound microenvironment for inhibiting skin fibrosis was investigated.
37321008	5	36	theme	significant	752:762	arg1	ability					764:770	significant ability	752:770	significant ability of scavenging excess intracellular ROS	752:809	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	11	37	theme	@	1725:1725	arg1	Mn-MSN					1726:1731	the prepared COS@Mn-MSN	1709:1731	the prepared COS@Mn-MSN	1709:1731	Therefore, the prepared COS@Mn-MSN shows great potential to active scarless wound therapy.
37321008	0	38	theme	immune	41:46	arg1	microenvironment					48:63	wound immune microenvironment	35:63	wound immune microenvironment	35:63	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	7	39	theme	whole	1202:1206	arg1	time					1208:1211	the whole time	1198:1211	the whole time	1198:1211	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	3	40	theme	@	488:488	arg1	Mn-MSN					489:494	COS@Mn-MSN	485:494	COS@Mn-MSN	485:494	In this work, a composite structure of Mn-Si-chitooligosaccharides (COS) was designed (COS@Mn-MSN) and the ability of regulating wound microenvironment for inhibiting skin fibrosis was investigated.
37321008	1	41	theme	skin	246:249	arg1	formation					260:268	excessive skin fibrosis formation	236:268	excessive skin fibrosis formation	236:268	Excessive oxidative stress and inflammation often impede wound healing and ultimately lead to excessive skin fibrosis formation.
37321008	10	42	theme	@	1603:1603	arg1	Mn-MSN					1604:1609	COS@Mn-MSN	1600:1609	COS@Mn-MSN	1600:1609	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	3	43	dep	designed	475:482	arg1	Mn-MSN					489:494	COS@Mn-MSN	485:494	COS@Mn-MSN	485:494	In this work, a composite structure of Mn-Si-chitooligosaccharides (COS) was designed (COS@Mn-MSN) and the ability of regulating wound microenvironment for inhibiting skin fibrosis was investigated.
37321008	10	44	theme	COS	1600:1602	arg1	Mn-MSN					1604:1609	COS@Mn-MSN	1600:1609	COS@Mn-MSN	1600:1609	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	4	45	theme	negative	620:627	arg1	effects					629:635	the negative effects	616:635	the negative effects of Mn	616:641	In order to reduce the negative effects of Mn, the nano-level Mn was doped into MSN to minimize its content.
37321008	5	46	theme	@	737:737	arg1	Mn-MSN					738:743	COS@Mn-MSN	734:743	COS@Mn-MSN	734:743	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	2	47	theme	surrounding	377:387	arg1	tissues					389:395	surrounding tissues	377:395	surrounding tissues	377:395	It was known that the structural properties of biomaterials can affect the healing and immune response of surrounding tissues.
37321008	6	48	theme	M2	865:866	arg1	polarization					879:890	M2 macrophage polarization	865:890	M2 macrophage polarization	865:890	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	2	49	theme	structural	293:302	arg1	properties					304:313	the structural properties	289:313	the structural properties of biomaterials	289:329	It was known that the structural properties of biomaterials can affect the healing and immune response of surrounding tissues.
37321008	7	50	theme	factors	1087:1093	arg1	expression					1055:1064	expression	1055:1064	expression of anti-inflammatory factors (IL-10 and CD206)	1055:1111	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	6	51	theme	@	847:847	arg1	Mn-MSN					848:853	COS@Mn-MSN	844:853	COS@Mn-MSN	844:853	The Si released from COS@Mn-MSN can shift M2 macrophage polarization in the later stage (1-3 d), showing anti-inflammatory effect.
37321008	4	52	theme	nano-level	648:657	arg1	Mn					659:660	the nano-level Mn	644:660	the nano-level Mn	644:660	In order to reduce the negative effects of Mn, the nano-level Mn was doped into MSN to minimize its content.
37321008	7	53	theme	COS	1026:1028	arg1	Mn-MSN					1030:1035	COS@Mn-MSN	1026:1035	COS@Mn-MSN	1026:1035	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	10	54	theme	oxidative	1505:1513	arg1	stress					1515:1520	oxidative stress	1505:1520	oxidative stress (0-1 d)	1505:1528	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	10	54	theme	oxidative	1505:1513	arg1	d					1527:1527	0-1 d	1523:1527	0-1 d	1523:1527	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	3	55	theme	skin	565:568	arg1	fibrosis					570:577	skin fibrosis	565:577	skin fibrosis	565:577	In this work, a composite structure of Mn-Si-chitooligosaccharides (COS) was designed (COS@Mn-MSN) and the ability of regulating wound microenvironment for inhibiting skin fibrosis was investigated.
37321008	5	56	from	Mn	728:729	arg1	Mn-MSN					738:743	COS@Mn-MSN	734:743	COS@Mn-MSN	734:743	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	9	57	theme	@	1390:1390	arg1	Mn-MSN					1391:1396	COS@Mn-MSN	1387:1396	COS@Mn-MSN	1387:1396	Besides, the inflammatory microenvironment mediated by COS@Mn-MSN downregulated Smad-7 gene expression and upregulated Col-1α gene expression.
37321008	1	58	theme	Excessive	142:150	arg1	stress					162:167	Excessive oxidative stress	142:167	Excessive oxidative stress	142:167	Excessive oxidative stress and inflammation often impede wound healing and ultimately lead to excessive skin fibrosis formation.
37321008	10	59	theme	skin	1635:1638	arg1	formation					1649:1657	excessive skin fibrosis formation	1625:1657	excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal	1625:1695	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	11	60	theme	prepared	1713:1720	arg1	Mn-MSN					1726:1731	the prepared COS@Mn-MSN	1709:1731	the prepared COS@Mn-MSN	1709:1731	Therefore, the prepared COS@Mn-MSN shows great potential to active scarless wound therapy.
37321008	9	61	theme	gene	1419:1422	arg1	expression					1424:1433	Smad-7 gene expression	1412:1433	Smad-7 gene expression	1412:1433	Besides, the inflammatory microenvironment mediated by COS@Mn-MSN downregulated Smad-7 gene expression and upregulated Col-1α gene expression.
37321008	0	62	theme	skin	89:92	arg1	fibrosis					94:101	excessive skin fibrosis	79:101	excessive skin fibrosis	79:101	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	0	63	from	effect	12:17	arg1	microenvironment					48:63	wound immune microenvironment	35:63	wound immune microenvironment	35:63	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	8	64	theme	associated	1241:1250	arg1	TGF-β1					1259:1264	fibrosis associated factor TGF-β1	1232:1264	fibrosis associated factor TGF-β1	1232:1264	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	9	65	theme	Col-1α	1451:1456	arg1	expression					1463:1472	Col-1α gene expression	1451:1472	Col-1α gene expression	1451:1472	Besides, the inflammatory microenvironment mediated by COS@Mn-MSN downregulated Smad-7 gene expression and upregulated Col-1α gene expression.
37321008	2	66	theme	biomaterials	318:329	arg1	properties					304:313	the structural properties	289:313	the structural properties of biomaterials	289:329	It was known that the structural properties of biomaterials can affect the healing and immune response of surrounding tissues.
37321008	8	67	theme	TGF-β1	1259:1264	arg1	expression					1218:1227	The expression	1214:1227	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929)	1214:1300	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	11	68	theme	COS	1722:1724	arg1	Mn-MSN					1726:1731	the prepared COS@Mn-MSN	1709:1731	the prepared COS@Mn-MSN	1709:1731	Therefore, the prepared COS@Mn-MSN shows great potential to active scarless wound therapy.
37321008	0	69	theme	ROS/TGF-β1/Smad7	117:132	arg1	signal					134:139	ROS/TGF-β1/Smad7 signal	117:139	ROS/TGF-β1/Smad7 signal	117:139	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	7	70	dep	factors	1087:1093	arg1	CD206					1106:1110	CD206	1106:1110	CD206	1106:1110	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	70	dep	factors	1087:1093	arg1	IL-10					1096:1100	IL-10	1096:1100	IL-10	1096:1100	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	70	dep	factors	1087:1093	arg1	factors					1087:1093	anti-inflammatory factors	1069:1093	anti-inflammatory factors (IL-10 and CD206)	1069:1111	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	8	71	theme	CD26	1270:1273	arg1	expression					1218:1227	The expression	1214:1227	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929)	1214:1300	The expression of fibrosis associated factor TGF-β1 and CD26 in fibroblast cells (L929) were inhibited by COS and Si.
37321008	5	72	theme	scavenging	775:784	arg1	ROS					807:809	scavenging excess intracellular ROS	775:809	scavenging excess intracellular ROS	775:809	The results show that Mn in COS@Mn-MSN showed significant ability of scavenging excess intracellular ROS within 1 d.
37321008	11	73	theme	active	1758:1763	arg1	therapy					1780:1786	active scarless wound therapy	1758:1786	active scarless wound therapy	1758:1786	Therefore, the prepared COS@Mn-MSN shows great potential to active scarless wound therapy.
37321008	2	74	dep	healing	346:352	arg1	the					342:344	the	342:344	the	342:344	It was known that the structural properties of biomaterials can affect the healing and immune response of surrounding tissues.
37321008	11	75	theme	scarless	1765:1772	arg1	therapy					1780:1786	active scarless wound therapy	1758:1786	active scarless wound therapy	1758:1786	Therefore, the prepared COS@Mn-MSN shows great potential to active scarless wound therapy.
37321008	0	76	theme	Mn-Si-COS	22:30	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis	0:101	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	3	77	theme	COS	485:487	arg1	Mn-MSN					489:494	COS@Mn-MSN	485:494	COS@Mn-MSN	485:494	In this work, a composite structure of Mn-Si-chitooligosaccharides (COS) was designed (COS@Mn-MSN) and the ability of regulating wound microenvironment for inhibiting skin fibrosis was investigated.
37321008	0	78	theme	wound	35:39	arg1	microenvironment					48:63	wound immune microenvironment	35:63	wound immune microenvironment	35:63	Synergistic effect of Mn-Si-COS on wound immune microenvironment by inhibiting excessive skin fibrosis mediated with ROS/TGF-β1/Smad7 signal.
37321008	7	79	theme	anti-inflammatory	1069:1085	arg1	CD206					1106:1110	CD206	1106:1110	CD206	1106:1110	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	79	theme	anti-inflammatory	1069:1085	arg1	IL-10					1096:1100	IL-10	1096:1100	IL-10	1096:1100	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	7	79	theme	anti-inflammatory	1069:1085	arg1	factors					1087:1093	anti-inflammatory factors	1069:1093	anti-inflammatory factors (IL-10 and CD206)	1069:1111	Macrophage (RAW264.7) were activated alternatively by COS released from COS@Mn-MSN, with upregulated expression of anti-inflammatory factors (IL-10 and CD206) and downregulated expression of pro-inflammatory factors (TNF-α, CD80, and IL-1β) in the whole time.
37321008	3	80	theme	composite	414:422	arg1	structure					424:432	a composite structure	412:432	a composite structure of Mn-Si-chitooligosaccharides (COS)	412:469	In this work, a composite structure of Mn-Si-chitooligosaccharides (COS) was designed (COS@Mn-MSN) and the ability of regulating wound microenvironment for inhibiting skin fibrosis was investigated.
37321008	2	81	theme	immune	358:363	arg1	response					365:372	immune response	358:372	immune response	358:372	It was known that the structural properties of biomaterials can affect the healing and immune response of surrounding tissues.
37321008	10	82	theme	ROS/TGF-β1/Smad7	1673:1688	arg1	signal					1690:1695	ROS/TGF-β1/Smad7 signal	1673:1695	ROS/TGF-β1/Smad7 signal	1673:1695	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	10	83	theme	anti-inflammatory	1565:1581	arg1	effects					1583:1589	anti-inflammatory effects	1565:1589	anti-inflammatory effects (0-3 d)	1565:1597	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	10	83	theme	anti-inflammatory	1565:1581	arg1	d					1596:1596	0-3 d	1592:1596	0-3 d	1592:1596	With the function of reducing oxidative stress (0-1 d), the TGF-β1 inhibition (1-3 d) and anti-inflammatory effects (0-3 d), COS@Mn-MSN could inhibit excessive skin fibrosis formation mediated with ROS/TGF-β1/Smad7 signal.
37321008	1	84	theme	fibrosis	251:258	arg1	formation					260:268	excessive skin fibrosis formation	236:268	excessive skin fibrosis formation	236:268	Excessive oxidative stress and inflammation often impede wound healing and ultimately lead to excessive skin fibrosis formation.
37321008	9	85	theme	COS	1387:1389	arg1	Mn-MSN					1391:1396	COS@Mn-MSN	1387:1396	COS@Mn-MSN	1387:1396	Besides, the inflammatory microenvironment mediated by COS@Mn-MSN downregulated Smad-7 gene expression and upregulated Col-1α gene expression.
37321008	2	86	theme	tissues	389:395	arg1	healing					346:352	healing	346:352	healing	346:352	It was known that the structural properties of biomaterials can affect the healing and immune response of surrounding tissues.
37321008	2	86	theme	tissues	389:395	arg1	response					365:372	immune response	358:372	immune response	358:372	It was known that the structural properties of biomaterials can affect the healing and immune response of surrounding tissues.
37146846	12	0	theme	junction	1670:1677	arg1	ZO-1					1689:1692	ZO-1	1689:1692	ZO-1	1689:1692	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	12	0	theme	junction	1670:1677	arg1	proteins					1679:1686	Tight junction proteins	1664:1686	Tight junction proteins (ZO-1 and occludin-1) in the small intestine	1664:1731	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	12	0	theme	junction	1670:1677	arg1	occludin-1					1698:1707	occludin-1	1698:1707	occludin-1	1698:1707	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	10	1	theme	necrotic	1418:1425	arg1	-α					1439:1440	tumor necrotic factor [TNF]-α	1412:1440	tumor necrotic factor [TNF]-α	1412:1440	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	17	2	theme	gut	2864:2866	arg1	microbiota					2868:2877	the gut microbiota	2860:2877	the gut microbiota	2860:2877	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	5	3	theme	cough	560:564	arg1	treatment					539:547	the treatment	535:547	the treatment of chronic cough, shortness of breath and phlegm, and chest distress	535:616	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	15	4	theme	forced	2216:2221	arg1	volume					2234:2239	forced expiratory volume	2216:2239	forced expiratory volume [FEV] 0.3	2216:2249	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	4	theme	forced	2216:2221	arg1	[FVC					2274:2277	forced vital capacity [FVC	2252:2277	forced vital capacity [FVC	2252:2277	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	7	5	theme	Pulvis	777:782	arg1	effect					748:753	the effect	744:753	the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism	744:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	10	6	theme	interleukin	1443:1453	arg1	[IL					1455:1457	interleukin [IL]-8	1443:1460	interleukin [IL]-8	1443:1460	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	5	7	theme	shortness	567:575	arg1	treatment					539:547	the treatment	535:547	the treatment of chronic cough, shortness of breath and phlegm, and chest distress	535:616	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	8	8	theme	microbiota	1070:1079	arg1	composition					1051:1061	the composition	1047:1061	the composition of gut microbiota and its metabolites	1047:1099	AIM OF THE STUDY To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.
37146846	16	9	theme	acetic	2627:2632	arg1	acid					2634:2637	acetic acid	2627:2637	acetic acid	2627:2637	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	11	10	theme	serum	1498:1502	arg1	LPS					1504:1506	the serum LPS and fluorescein isothiocyanate-dextran	1494:1545	LPS	1504:1506	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	16	11	theme	occludin-1	2717:2726	arg1	expression					2694:2703	the expression	2690:2703	the expression of ZO-1 and occludin-1 in the small intestine of COPD rats	2690:2762	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	12	12	theme	barrier	1859:1865	arg1	function					1867:1874	the intestinal barrier function	1844:1874	the intestinal barrier function	1844:1874	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	14	13	theme	gut	2088:2090	arg1	microbiota					2092:2101	the gut microbiota	2084:2101	the gut microbiota of COPD rats	2084:2114	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	10	14	theme	lung	1342:1345	arg1	alteration					1360:1369	lung histological alteration	1342:1369	lung histological alteration	1342:1369	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	6	15	theme	lung	685:688	arg1	inflammation					690:701	lung inflammation	685:701	lung inflammation	685:701	Past studies demonstrated that Seabuckthorn Wuwei Pulvis improved lung inflammation and chronic bronchitis in mice.
37146846	15	16	theme	expiratory	2223:2232	arg1	volume					2234:2239	forced expiratory volume	2216:2239	forced expiratory volume [FEV] 0.3	2216:2249	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	16	theme	expiratory	2223:2232	arg1	[FVC					2274:2277	forced vital capacity [FVC	2252:2277	forced vital capacity [FVC	2252:2277	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	13	17	from	contents	1881:1888	arg1	feces					1932:1936	the feces	1928:1936	the feces of rats	1928:1944	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	16	18	theme	acid	2650:2653	arg1	productions					2612:2622	the productions	2608:2622	the productions of acetic acid, propionic acid, and butyric acid	2608:2671	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	15	19	from	levels	2312:2317	arg1	lung					2358:2361	the lung	2354:2361	the lung	2354:2361	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	20	theme	cells	2412:2416	arg1	infiltration					2383:2394	the infiltration	2379:2394	the infiltration of inflammatory cells into the lung	2379:2430	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	1	21	theme	Mongolian	215:223	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis	135:190	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP)	135:196	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	1	21	theme	Mongolian	215:223	arg1	medicine					225:232	a traditional Mongolian medicine	201:232	a traditional Mongolian medicine used in China	201:246	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	16	22	from	expression	2694:2703	arg1	intestine					2741:2749	the small intestine	2731:2749	the small intestine of COPD rats	2731:2762	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	16	23	theme	gut	2491:2493	arg1	microbiota					2495:2504	gut microbiota	2491:2504	gut microbiota	2491:2504	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	5	24	theme	distress	609:616	arg1	treatment					539:547	the treatment	535:547	the treatment of chronic cough, shortness of breath and phlegm, and chest distress	535:616	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	15	25	theme	TNF-α	2322:2326	arg1	levels					2312:2317	the levels	2308:2317	the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung	2308:2361	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	17	26	from	function	2949:2956	arg1	rats					2961:2964	rats	2961:2964	rats with COPD induced by LPS and smoking	2961:3001	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	14	27	theme	throughput	2017:2026	arg1	sequencing					2028:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	5	28	theme	breath	580:585	arg1	cough					560:564	chronic cough	552:564	chronic cough	552:564	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	5	28	theme	breath	580:585	arg1	distress					609:616	chest distress	603:616	chest distress	603:616	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	5	28	theme	breath	580:585	arg1	shortness					567:575	shortness	567:575	shortness of breath and phlegm	567:596	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	9	29	theme	Pulvis	1158:1163	arg1	effects					1128:1134	The effects	1124:1134	MATERIALS AND METHODS The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model	1102:1183	MATERIALS AND METHODS The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model were established by exposure to lipopolysaccharide (LPS) and smoking.
37146846	16	30	theme	low	2437:2439	arg1	doses					2452:2456	The low and median doses	2433:2456	The low and median doses of SWP	2433:2463	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	16	31	theme	Aerococcaceae	2583:2595	arg1	abundances					2527:2536	the abundances	2523:2536	the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae	2523:2595	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	5	32	theme	phlegm	591:596	arg1	cough					560:564	chronic cough	552:564	chronic cough	552:564	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	5	32	theme	phlegm	591:596	arg1	distress					609:616	chest distress	603:616	chest distress	603:616	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	5	32	theme	phlegm	591:596	arg1	shortness					567:575	shortness	567:575	shortness of breath and phlegm	567:596	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	13	33	theme	chromatography-mass	1969:1987	arg1	spectrometry					1989:2000	gas chromatography-mass spectrometry	1965:2000	gas chromatography-mass spectrometry	1965:2000	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	17	34	theme	CONCLUSION	2765:2774	arg1	SWP					2776:2778	CONCLUSION SWP	2765:2778	CONCLUSION SWP	2765:2778	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	11	35	theme	immunosorbent	1594:1606	arg1	assay					1608:1612	an enzyme-linked immunosorbent assay	1577:1612	an enzyme-linked immunosorbent assay	1577:1612	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	9	36	theme	rat	1175:1177	arg1	model					1179:1183	a COPD rat model	1168:1183	a COPD rat model	1168:1183	MATERIALS AND METHODS The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model were established by exposure to lipopolysaccharide (LPS) and smoking.
37146846	14	37	theme	high	2012:2015	arg1	sequencing					2028:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	8	38	theme	STUDY	912:916	arg1	AIM					901:903	AIM	901:903	AIM OF THE STUDY	901:916	AIM OF THE STUDY To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.
37146846	7	39	theme	action	859:864	arg1	mechanism					866:874	the underlying action mechanism	844:874	the underlying action mechanism	844:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	16	40	theme	ZO-1	2708:2711	arg1	expression					2694:2703	the expression	2690:2703	the expression of ZO-1 and occludin-1 in the small intestine of COPD rats	2690:2762	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	9	41	dep	MATERIALS	1102:1110	arg1	effects					1128:1134	The effects	1124:1134	MATERIALS AND METHODS The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model	1102:1183	MATERIALS AND METHODS The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model were established by exposure to lipopolysaccharide (LPS) and smoking.
37146846	10	42	theme	pulmonary	1322:1330	arg1	function					1332:1339	pulmonary function	1322:1339	pulmonary function	1322:1339	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	11	43	theme	microplate	1631:1640	arg1	reader					1642:1647	fluorescence microplate reader	1618:1647	fluorescence microplate reader	1618:1647	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	0	44	theme	microbiota-short	94:109	arg1	acids					123:127	gut microbiota-short chain fatty acids	90:127	gut microbiota-short chain fatty acids axis	90:132	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	15	45	theme	capacity	2265:2272	arg1	[FVC					2274:2277	forced vital capacity [FVC	2252:2277	forced vital capacity [FVC	2252:2277	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	45	theme	capacity	2265:2272	arg1	volume					2234:2239	forced expiratory volume	2216:2239	forced expiratory volume [FEV] 0.3	2216:2249	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	3	46	theme	dry	331:333	arg1	root					335:338	dry root	331:338	dry root	331:338	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	3	46	theme	dry	331:333	arg1	25 g					341:344	25 g	341:344	25 g	341:344	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	0	47	theme	fatty	117:121	arg1	acids					123:127	gut microbiota-short chain fatty acids	90:127	gut microbiota-short chain fatty acids axis	90:132	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	15	48	theme	forced	2252:2257	arg1	capacity					2265:2272	forced vital capacity	2252:2272	forced vital capacity [FVC	2252:2277	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	8	49	theme	anti-COPD	934:942	arg1	effect					944:949	the anti-COPD effect	930:949	the anti-COPD effect of Seabuckthorn Wuwei Pulvis	930:978	AIM OF THE STUDY To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.
37146846	15	50	theme	IL-6	2335:2338	arg1	levels					2312:2317	the levels	2308:2317	the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung	2308:2361	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	9	51	from	effects	1128:1134	arg1	model					1179:1183	a COPD rat model	1168:1183	a COPD rat model	1168:1183	MATERIALS AND METHODS The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model were established by exposure to lipopolysaccharide (LPS) and smoking.
37146846	3	52	theme	Vitis	348:352	arg1	vinifera					354:361	Vitis vinifera	348:361	Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch	348:420	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	15	53	theme	low	2140:2142	arg1	doses					2155:2159	low and median doses	2140:2159	low and median doses of SWP	2140:2166	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	10	54	theme	histological	1347:1358	arg1	alteration					1360:1369	lung histological alteration	1342:1369	lung histological alteration	1342:1369	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	17	55	theme	inflammatory	2827:2838	arg1	response					2840:2847	the inflammatory response	2823:2847	the inflammatory response	2823:2847	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	12	56	theme	quantitative	1771:1782	arg1	reactions					1801:1809	real-time quantitative polymerase chain reactions	1761:1809	real-time quantitative polymerase chain reactions	1761:1809	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	15	57	theme	IL-8	2329:2332	arg1	levels					2312:2317	the levels	2308:2317	the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung	2308:2361	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	58	with	Treatment	2125:2133	arg1	doses					2155:2159	low and median doses	2140:2159	low and median doses of SWP	2140:2166	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	16	59	theme	COPD	2754:2757	arg1	rats					2759:2762	COPD rats	2754:2762	COPD rats	2754:2762	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	4	60	theme	ripe	488:491	arg1	10 g					500:503	10 g	500:503	10 g	500:503	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	4	60	theme	ripe	488:491	arg1	fruit					493:497	desiccative ripe fruit	476:497	desiccative ripe fruit	476:497	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	14	61	theme	rats	2111:2114	arg1	microbiota					2092:2101	the gut microbiota	2084:2101	the gut microbiota of COPD rats	2084:2114	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	0	62	theme	chronic	37:43	arg1	disease					67:73	chronic obstructive pulmonary disease	37:73	chronic obstructive pulmonary disease in rat	37:80	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	6	63	from	inflammation	690:701	arg1	mice					729:732	mice	729:732	mice	729:732	Past studies demonstrated that Seabuckthorn Wuwei Pulvis improved lung inflammation and chronic bronchitis in mice.
37146846	10	64	theme	tumor	1412:1416	arg1	-α					1439:1440	tumor necrotic factor [TNF]-α	1412:1440	tumor necrotic factor [TNF]-α	1412:1440	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	13	65	theme	acids	1911:1915	arg1	contents					1881:1888	The contents	1877:1888	The contents of short-chain fatty acids (SCFAs) in the feces of rats	1877:1944	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	0	66	theme	pulmonary	57:65	arg1	disease					67:73	chronic obstructive pulmonary disease	37:73	chronic obstructive pulmonary disease in rat	37:80	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	4	67	dep	Ellis	469:473	arg1	10 g					500:503	10 g	500:503	10 g	500:503	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	4	67	dep	Ellis	469:473	arg1	fruit					493:497	desiccative ripe fruit	476:497	desiccative ripe fruit	476:497	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	16	68	theme	median	2445:2450	arg1	doses					2452:2456	The low and median doses	2433:2456	The low and median doses of SWP	2433:2463	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	10	69	theme	factors	1403:1409	arg1	function					1332:1339	pulmonary function	1322:1339	pulmonary function	1322:1339	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	10	69	theme	factors	1403:1409	arg1	levels					1380:1385	the levels	1376:1385	the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17)	1376:1478	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	10	69	theme	factors	1403:1409	arg1	alteration					1360:1369	lung histological alteration	1342:1369	lung histological alteration	1342:1369	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	10	69	theme	factors	1403:1409	arg1	weight					1314:1319	the animal weight	1303:1319	the animal weight	1303:1319	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	11	70	theme	enzyme-linked	1580:1592	arg1	assay					1608:1612	an enzyme-linked immunosorbent assay	1577:1612	an enzyme-linked immunosorbent assay	1577:1612	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	7	71	from	effect	748:753	arg1	disease					817:823	chronic obstructive pulmonary disease	787:823	chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism	787:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	7	71	from	effect	748:753	arg1	COPD					826:829	COPD	826:829	COPD	826:829	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	15	72	theme	inflammatory	2399:2410	arg1	cells					2412:2416	inflammatory cells	2399:2416	inflammatory cells	2399:2416	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	73	theme	pulmonary	2196:2204	arg1	function					2206:2213	the pulmonary function	2192:2213	the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC]	2192:2278	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	8	74	theme	ameliorative	1008:1019	arg1	effect					1021:1026	its ameliorative effect	1004:1026	its ameliorative effect	1004:1026	AIM OF THE STUDY To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.
37146846	12	75	theme	Tight	1664:1668	arg1	ZO-1					1689:1692	ZO-1	1689:1692	ZO-1	1689:1692	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	12	75	theme	Tight	1664:1668	arg1	proteins					1679:1686	Tight junction proteins	1664:1686	Tight junction proteins (ZO-1 and occludin-1) in the small intestine	1664:1731	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	12	75	theme	Tight	1664:1668	arg1	occludin-1					1698:1707	occludin-1	1698:1707	occludin-1	1698:1707	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	14	76	theme	COPD	2106:2109	arg1	rats					2111:2114	COPD rats	2106:2114	COPD rats	2106:2114	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	10	77	theme	factor	1427:1432	arg1	-α					1439:1440	tumor necrotic factor [TNF]-α	1412:1440	tumor necrotic factor [TNF]-α	1412:1440	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	5	78	theme	chronic	552:558	arg1	cough					560:564	chronic cough	552:564	chronic cough	552:564	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	13	79	theme	short-chain	1893:1903	arg1	acids					1911:1915	short-chain fatty acids	1893:1915	short-chain fatty acids (SCFAs)	1893:1923	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	13	79	theme	short-chain	1893:1903	arg1	SCFAs					1918:1922	SCFAs	1918:1922	SCFAs	1918:1922	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	7	80	theme	obstructive	795:805	arg1	disease					817:823	chronic obstructive pulmonary disease	787:823	chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism	787:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	7	80	theme	obstructive	795:805	arg1	COPD					826:829	COPD	826:829	COPD	826:829	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	15	81	theme	SWP	2164:2166	arg1	doses					2155:2159	low and median doses	2140:2159	low and median doses of SWP	2140:2166	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	17	82	theme	pulmonary	2789:2797	arg1	functions					2799:2807	pulmonary functions	2789:2807	pulmonary functions	2789:2807	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	14	83	theme	SWP	2077:2079	arg1	effect					2067:2072	the effect	2063:2072	the effect of SWP on the gut microbiota of COPD rats	2063:2114	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	11	84	theme	fluorescein	1512:1522	arg1	isothiocyanate-dextran					1524:1545	the serum LPS and fluorescein isothiocyanate-dextran	1494:1545	isothiocyanate-dextran	1524:1545	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	6	85	theme	chronic	707:713	arg1	bronchitis					715:724	chronic bronchitis	707:724	chronic bronchitis	707:724	Past studies demonstrated that Seabuckthorn Wuwei Pulvis improved lung inflammation and chronic bronchitis in mice.
37146846	8	86	theme	gut	1066:1068	arg1	microbiota					1070:1079	gut microbiota	1066:1079	gut microbiota	1066:1079	AIM OF THE STUDY To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.
37146846	15	87	dep	RESULTS	2117:2123	arg1	decreased					2298:2306	decreased	2298:2306	decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung	2298:2361	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	87	dep	RESULTS	2117:2123	arg1	attenuated					2368:2377	attenuated	2368:2377	attenuated the infiltration of inflammatory cells into the lung	2368:2430	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	87	dep	RESULTS	2117:2123	arg1	increased					2182:2190	increased	2182:2190	increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC)	2182:2295	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	11	88	theme	LPS	1504:1506	arg1	levels					1547:1552	the serum LPS and fluorescein isothiocyanate-dextran levels	1494:1552	the serum LPS and fluorescein isothiocyanate-dextran levels	1494:1552	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	16	89	theme	small	2735:2739	arg1	intestine					2741:2749	the small intestine	2731:2749	the small intestine of COPD rats	2731:2762	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	10	90	theme	animal	1307:1312	arg1	weight					1314:1319	the animal weight	1303:1319	the animal weight	1303:1319	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	17	91	theme	SCFA	2891:2894	arg1	production					2896:2905	SCFA production	2891:2905	SCFA production	2891:2905	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	12	92	theme	intestinal	1848:1857	arg1	function					1867:1874	the intestinal barrier function	1844:1874	the intestinal barrier function	1844:1874	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	16	93	theme	acid	2634:2637	arg1	productions					2612:2622	the productions	2608:2622	the productions of acetic acid, propionic acid, and butyric acid	2608:2671	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	12	94	theme	chain	1795:1799	arg1	reactions					1801:1809	real-time quantitative polymerase chain reactions	1761:1809	real-time quantitative polymerase chain reactions	1761:1809	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	1	95	theme	traditional	203:213	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis	135:190	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP)	135:196	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	1	95	theme	traditional	203:213	arg1	medicine					225:232	a traditional Mongolian medicine	201:232	a traditional Mongolian medicine used in China	201:246	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	8	96	theme	metabolites	1089:1099	arg1	composition					1051:1061	the composition	1047:1061	the composition of gut microbiota and its metabolites	1047:1099	AIM OF THE STUDY To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.
37146846	14	97	from	effect	2067:2072	arg1	microbiota					2092:2101	the gut microbiota	2084:2101	the gut microbiota of COPD rats	2084:2114	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	16	98	theme	propionic	2640:2648	arg1	acid					2650:2653	propionic acid	2640:2653	propionic acid	2640:2653	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	5	99	theme	chest	603:607	arg1	distress					609:616	chest distress	603:616	chest distress	603:616	It is clinically applied in the treatment of chronic cough, shortness of breath and phlegm, and chest distress.
37146846	16	100	theme	Ruminococcaceae	2541:2555	arg1	abundances					2527:2536	the abundances	2523:2536	the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae	2523:2595	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	16	101	theme	Christensenellaceae	2558:2576	arg1	abundances					2527:2536	the abundances	2523:2536	the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae	2523:2595	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	14	102	theme	16S	2003:2005	arg1	sequencing					2028:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	13	103	theme	gas	1965:1967	arg1	spectrometry					1989:2000	gas chromatography-mass spectrometry	1965:2000	gas chromatography-mass spectrometry	1965:2000	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	1	104	theme	ETHNOPHARMACOLOGICAL	135:154	arg1	SWP					193:195	SWP	193:195	SWP	193:195	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	1	104	theme	ETHNOPHARMACOLOGICAL	135:154	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis	135:190	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP)	135:196	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	1	104	theme	ETHNOPHARMACOLOGICAL	135:154	arg1	medicine					225:232	a traditional Mongolian medicine	201:232	a traditional Mongolian medicine used in China	201:246	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	11	105	theme	fluorescence	1618:1629	arg1	reader					1642:1647	fluorescence microplate reader	1618:1647	fluorescence microplate reader	1618:1647	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	10	106	dep	factors	1403:1409	arg1	IL-6					1463:1466	IL-6	1463:1466	IL-6	1463:1466	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	10	106	dep	factors	1403:1409	arg1	IL-17					1473:1477	IL-17	1473:1477	IL-17	1473:1477	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	10	106	dep	factors	1403:1409	arg1	-α					1439:1440	tumor necrotic factor [TNF]-α	1412:1440	tumor necrotic factor [TNF]-α	1412:1440	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	10	106	dep	factors	1403:1409	arg1	[IL					1455:1457	interleukin [IL]-8	1443:1460	interleukin [IL]-8	1443:1460	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	9	107	theme	COPD	1170:1173	arg1	model					1179:1183	a COPD rat model	1168:1183	a COPD rat model	1168:1183	MATERIALS AND METHODS The effects of Seabuckthorn Wuwei Pulvis on a COPD rat model were established by exposure to lipopolysaccharide (LPS) and smoking.
37146846	17	108	with	rats	2961:2964	arg1	COPD					2971:2974	COPD	2971:2974	COPD induced by LPS and smoking	2971:3001	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	0	109	theme	gut	90:92	arg1	acids					123:127	gut microbiota-short chain fatty acids	90:127	gut microbiota-short chain fatty acids axis	90:132	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	13	110	theme	rats	1941:1944	arg1	feces					1932:1936	the feces	1928:1936	the feces of rats	1928:1944	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	4	111	theme	dry	424:426	arg1	root					428:431	dry root	424:431	dry root	424:431	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	4	111	theme	dry	424:426	arg1	15 g					434:437	15 g	434:437	15 g	434:437	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	14	112	theme	rDNA	2007:2010	arg1	sequencing					2028:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	2	113	dep	berries	289:295	arg1	30 g					298:301	30 g	298:301	30 g	298:301	It is composed of Hippophae rhamnoides (berries, 30 g), Aucklandiae costus Falc.
37146846	0	114	theme	chain	111:115	arg1	acids					123:127	gut microbiota-short chain fatty acids	90:127	gut microbiota-short chain fatty acids axis	90:132	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	12	115	theme	Western	1815:1821	arg1	blotting					1823:1830	Western blotting	1815:1830	Western blotting	1815:1830	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	0	116	from	disease	67:73	arg1	rat					78:80	rat	78:80	rat	78:80	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	3	117	dep	vinifera	354:361	arg1	root					335:338	dry root	331:338	dry root	331:338	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	3	117	dep	vinifera	354:361	arg1	25 g					341:344	25 g	341:344	25 g	341:344	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	3	117	dep	vinifera	354:361	arg1	Fisch					416:420	Fisch	416:420	Fisch	416:420	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	3	117	dep	vinifera	354:361	arg1	berries					378:384	berries	378:384	berries	378:384	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	3	117	dep	vinifera	354:361	arg1	Cordifolia					366:375	Cordifolia	366:375	Cordifolia	366:375	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	1	118	dep	RELEVANCE	156:164	arg1	Pulvis					185:190	Pulvis	185:190	Pulvis	185:190	ETHNOPHARMACOLOGICAL RELEVANCE Seabuckthorn Wuwei Pulvis (SWP) is a traditional Mongolian medicine used in China.
37146846	12	119	dep	proteins	1679:1686	arg1	ZO-1					1689:1692	ZO-1	1689:1692	ZO-1	1689:1692	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	12	119	dep	proteins	1679:1686	arg1	proteins					1679:1686	Tight junction proteins	1664:1686	Tight junction proteins (ZO-1 and occludin-1) in the small intestine	1664:1731	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	12	119	dep	proteins	1679:1686	arg1	occludin-1					1698:1707	occludin-1	1698:1707	occludin-1	1698:1707	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	0	120	theme	acids	123:127	arg1	axis					129:132	gut microbiota-short chain fatty acids axis	90:132	gut microbiota-short chain fatty acids axis	90:132	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	12	121	theme	real-time	1761:1769	arg1	reactions					1801:1809	real-time quantitative polymerase chain reactions	1761:1809	real-time quantitative polymerase chain reactions	1761:1809	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	7	122	theme	underlying	848:857	arg1	mechanism					866:874	the underlying action mechanism	844:874	the underlying action mechanism	844:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	6	123	from	bronchitis	715:724	arg1	mice					729:732	mice	729:732	mice	729:732	Past studies demonstrated that Seabuckthorn Wuwei Pulvis improved lung inflammation and chronic bronchitis in mice.
37146846	15	124	theme	vital	2259:2263	arg1	capacity					2265:2272	forced vital capacity	2252:2272	forced vital capacity [FVC	2252:2277	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	12	125	theme	polymerase	1784:1793	arg1	reactions					1801:1809	real-time quantitative polymerase chain reactions	1761:1809	real-time quantitative polymerase chain reactions	1761:1809	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	16	126	theme	acid	2668:2671	arg1	productions					2612:2622	the productions	2608:2622	the productions of acetic acid, propionic acid, and butyric acid	2608:2671	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	4	127	theme	desiccative	476:486	arg1	10 g					500:503	10 g	500:503	10 g	500:503	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	4	127	theme	desiccative	476:486	arg1	fruit					493:497	desiccative ripe fruit	476:497	desiccative ripe fruit	476:497	(dry root, 15 g), and Gardenia jasminoides J. Ellis (desiccative ripe fruit, 10 g).
37146846	3	128	dep	berries	378:384	arg1	20 g					387:390	20 g	387:390	20 g	387:390	(dry root, 25 g), Vitis vinifera F. Cordifolia (berries, 20 g), Glycyrrhiza uralensis Fisch.
37146846	7	129	from	disease	817:823	arg1	rats					835:838	rats	835:838	rats	835:838	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	7	129	from	disease	817:823	arg1	mechanism					866:874	the underlying action mechanism	844:874	the underlying action mechanism	844:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	16	130	theme	rats	2759:2762	arg1	intestine					2741:2749	the small intestine	2731:2749	the small intestine of COPD rats	2731:2762	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	15	131	theme	median	2148:2153	arg1	doses					2155:2159	low and median doses	2140:2159	low and median doses of SWP	2140:2166	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	8	132	theme	Pulvis	973:978	arg1	effect					944:949	the anti-COPD effect	930:949	the anti-COPD effect of Seabuckthorn Wuwei Pulvis	930:978	AIM OF THE STUDY To evaluate the anti-COPD effect of Seabuckthorn Wuwei Pulvis and investigate whether its ameliorative effect is correlated with the composition of gut microbiota and its metabolites.
37146846	15	133	theme	IL-17	2345:2349	arg1	levels					2312:2317	the levels	2308:2317	the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung	2308:2361	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	17	134	theme	barrier	2941:2947	arg1	function					2949:2956	the intestinal barrier function	2926:2956	the intestinal barrier function in rats with COPD induced by LPS and smoking	2926:3001	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	0	135	theme	obstructive	45:55	arg1	disease					67:73	chronic obstructive pulmonary disease	37:73	chronic obstructive pulmonary disease in rat	37:80	Seabuckthorn Wuwei Pulvis attenuates chronic obstructive pulmonary disease in rat through gut microbiota-short chain fatty acids axis.
37146846	14	136	used	used	2043:2046	arg2	sequencing					2028:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing	2003:2037	16S rDNA high throughput sequencing was used to investigate the effect of SWP on the gut microbiota of COPD rats.
37146846	16	137	theme	microbiota	2495:2504	arg1	composition					2476:2486	the composition	2472:2486	the composition	2472:2486	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	11	138	theme	isothiocyanate-dextran	1524:1545	arg1	levels					1547:1552	the serum LPS and fluorescein isothiocyanate-dextran levels	1494:1552	the serum LPS and fluorescein isothiocyanate-dextran levels	1494:1552	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	13	139	theme	fatty	1905:1909	arg1	acids					1911:1915	short-chain fatty acids	1893:1915	short-chain fatty acids (SCFAs)	1893:1923	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	13	139	theme	fatty	1905:1909	arg1	SCFAs					1918:1922	SCFAs	1918:1922	SCFAs	1918:1922	The contents of short-chain fatty acids (SCFAs) in the feces of rats were determined by gas chromatography-mass spectrometry.
37146846	15	140	dep	function	2206:2213	arg1	volume					2234:2239	forced expiratory volume	2216:2239	forced expiratory volume [FEV] 0.3	2216:2249	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	15	140	dep	function	2206:2213	arg1	[FVC					2274:2277	forced vital capacity [FVC	2252:2277	forced vital capacity [FVC	2252:2277	RESULTS Treatment with low and median doses of SWP significantly increased the pulmonary function (forced expiratory volume [FEV] 0.3, forced vital capacity [FVC], and FEV0.3/FVC), decreased the levels of TNF-α, IL-8, IL-6, and IL-17 in the lung, and attenuated the infiltration of inflammatory cells into the lung.
37146846	16	141	theme	butyric	2660:2666	arg1	acid					2668:2671	butyric acid	2660:2671	butyric acid	2660:2671	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	17	142	theme	intestinal	2930:2939	arg1	function					2949:2956	the intestinal barrier function	2926:2956	the intestinal barrier function in rats with COPD induced by LPS and smoking	2926:3001	CONCLUSION SWP improved pulmonary functions and inhibited the inflammatory response by shaping the gut microbiota, increasing SCFA production, and strengthening the intestinal barrier function in rats with COPD induced by LPS and smoking.
37146846	12	143	theme	small	1717:1721	arg1	intestine					1723:1731	the small intestine	1713:1731	the small intestine	1713:1731	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	16	144	theme	SWP	2461:2463	arg1	doses					2452:2456	The low and median doses	2433:2456	The low and median doses of SWP	2433:2463	The low and median doses of SWP shaped the composition of gut microbiota, which increased the abundances of Ruminococcaceae, Christensenellaceae, and Aerococcaceae, increased the productions of acetic acid, propionic acid, and butyric acid, and upregulated the expression of ZO-1 and occludin-1 in the small intestine of COPD rats.
37146846	10	145	theme	inflammatory	1390:1401	arg1	factors					1403:1409	inflammatory factors	1390:1409	inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17)	1390:1478	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	7	146	theme	pulmonary	807:815	arg1	disease					817:823	chronic obstructive pulmonary disease	787:823	chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism	787:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	7	146	theme	pulmonary	807:815	arg1	COPD					826:829	COPD	826:829	COPD	826:829	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	12	147	from	proteins	1679:1686	arg1	intestine					1723:1731	the small intestine	1713:1731	the small intestine	1713:1731	Tight junction proteins (ZO-1 and occludin-1) in the small intestine were detected by performing real-time quantitative polymerase chain reactions and Western blotting to evaluate the intestinal barrier function.
37146846	11	148	link	enzyme-linked	1580:1592	arg1	assay					1608:1612	an enzyme-linked immunosorbent assay	1577:1612	an enzyme-linked immunosorbent assay	1577:1612	Furthermore, the serum LPS and fluorescein isothiocyanate-dextran levels were detected by using an enzyme-linked immunosorbent assay and fluorescence microplate reader, respectively.
37146846	10	149	theme	[TNF	1434:1437	arg1	-α					1439:1440	tumor necrotic factor [TNF]-α	1412:1440	tumor necrotic factor [TNF]-α	1412:1440	These effects were then evaluated by monitoring the animal weight, pulmonary function, lung histological alteration, and the levels of inflammatory factors (tumor necrotic factor [TNF]-α, interleukin [IL]-8, IL-6, and IL-17).
37146846	6	150	theme	Past	619:622	arg1	studies					624:630	Past studies	619:630	Past studies	619:630	Past studies demonstrated that Seabuckthorn Wuwei Pulvis improved lung inflammation and chronic bronchitis in mice.
37146846	7	151	theme	chronic	787:793	arg1	disease					817:823	chronic obstructive pulmonary disease	787:823	chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism	787:874	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
37146846	7	151	theme	chronic	787:793	arg1	COPD					826:829	COPD	826:829	COPD	826:829	However, the effect of Seabuckthorn Wuwei Pulvis on chronic obstructive pulmonary disease (COPD) in rats and the underlying action mechanism is not fully understood.
35609046	12	0	theme	cats	1706:1709	arg1	mass					1675:1678	total body mass	1664:1678	total body mass	1664:1678	Test food did not affect total body mass or composition of healthy cats.
35609046	12	0	theme	cats	1706:1709	arg1	composition					1683:1693	composition	1683:1693	composition	1683:1693	Test food did not affect total body mass or composition of healthy cats.
35609046	14	1	theme	acid	2154:2157	arg1	concentrations					2120:2133	increased concentrations	2110:2133	increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05)	2110:2210	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	1	theme	acid	2154:2157	arg1	concentrations					2052:2065	significantly decreased concentrations	2028:2065	significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004)	2028:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	4	2	theme	balanced	498:505	arg1	food					459:462	The pre-trial food	445:462	The pre-trial food (control food)	445:477	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	4	2	theme	balanced	498:505	arg1	food					511:514	a complete and balanced dry food	483:514	a complete and balanced dry food designed to aid in the management of CKD	483:555	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	5	3	theme	Test	558:561	arg1	food					584:587	the control food	572:587	the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	572:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	3	theme	Test	558:561	arg1	food					563:566	Test food	558:566	Test food	558:566	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	11	4	theme	food	1578:1581	arg1	intake					1583:1588	food intake	1578:1588	food intake	1578:1588	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	9	5	theme	feeding	1152:1158	arg1	period					1160:1165	each feeding period	1147:1165	each feeding period	1147:1165	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	12	6	theme	body	1670:1673	arg1	mass					1675:1678	total body mass	1664:1678	total body mass	1664:1678	Test food did not affect total body mass or composition of healthy cats.
35609046	15	7	theme	CKD	2486:2488	arg1	cats					2490:2493	CKD cats	2486:2493	CKD cats	2486:2493	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	13	8	theme	test	1825:1828	arg1	food					1830:1833	test food	1825:1833	test food	1825:1833	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	14	9	theme	increased	2110:2118	arg1	concentrations					2120:2133	increased concentrations	2110:2133	increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05)	2110:2210	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	0	10	theme	Feeding	0:6	arg1	cats					8:11	Feeding cats	0:11	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics	0:85	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	12	11	theme	healthy	1698:1704	arg1	cats					1706:1709	healthy cats	1698:1709	healthy cats	1698:1709	Test food did not affect total body mass or composition of healthy cats.
35609046	15	12	theme	total	2467:2471	arg1	mass					2478:2481	increased total body mass	2457:2481	increased total body mass in CKD cats	2457:2493	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	10	13	theme	dual-energy	1337:1347	arg1	DEXA					1371:1374	DEXA	1371:1374	DEXA	1371:1374	Body weight and composition were measured using dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period.
35609046	10	13	theme	dual-energy	1337:1347	arg1	absorptiometry					1355:1368	dual-energy X-ray absorptiometry	1337:1368	dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period	1337:1422	Body weight and composition were measured using dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period.
35609046	7	14	theme	cross-over	880:889	arg1	design					897:902	a cross-over study design	878:902	a cross-over study design	878:902	Each food was fed for 8 weeks in a cross-over study design.
35609046	14	15	theme	test	2006:2009	arg1	food					2011:2014	consuming test food	1996:2014	consuming test food	1996:2014	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	10	16	dep	absorptiometry	1355:1368	arg1	scan					1377:1380	scan	1377:1380	scan at baseline and after each feeding period	1377:1422	Body weight and composition were measured using dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period.
35609046	11	17	theme	control	1518:1524	arg1	0.004					1536:1540	P = 0.004	1532:1540	P = 0.004	1532:1540	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	11	17	theme	control	1518:1524	arg1	food					1526:1529	control food	1518:1529	control food (P = 0.004)	1518:1541	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	2	18	theme	body	254:257	arg1	weight					259:264	body weight	254:264	body weight	254:264	As CKD advances, loss of body weight contributes to morbidity and mortality.
35609046	13	19	theme	test	1968:1971	arg1	food					1973:1976	consuming test food	1958:1976	consuming test food	1958:1976	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	4	20	theme	CKD	553:555	arg1	management					539:548	the management	535:548	the management of CKD	535:555	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	0	21	theme	uremic	125:130	arg1	toxins					132:137	uremic toxins	125:137	uremic toxins	125:137	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	5	22	theme	%	685:685	arg1	scFOS					723:727	scFOS	723:727	scFOS	723:727	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	22	theme	%	685:685	arg1	fructooligosaccharides					699:720	0.407% short chain fructooligosaccharides	680:720	0.407% short chain fructooligosaccharides (scFOS)	680:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	22	theme	%	685:685	arg1	prebiotics					628:637	prebiotics	628:637	prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	628:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	1	23	theme	decreased	186:194	arg1	ability					196:202	a decreased ability	184:202	a decreased ability to maintain body weight	184:226	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	12	24	theme	Test	1639:1642	arg1	food					1644:1647	Test food	1639:1647	Test food	1639:1647	Test food did not affect total body mass or composition of healthy cats.
35609046	8	25	theme	control	946:952	arg1	food					954:957	control food or test food	946:970	food	954:957	In a second study, healthy cats received control food or test food for 8 weeks (n = 8 each group).
35609046	11	26	theme	=	1534:1534	arg1	0.004					1536:1540	P = 0.004	1532:1540	P = 0.004	1532:1540	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	11	26	theme	=	1534:1534	arg1	food					1526:1529	control food	1518:1529	control food (P = 0.004)	1518:1541	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	5	27	theme	control	576:582	arg1	food					584:587	the control food	572:587	the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	572:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	27	theme	control	576:582	arg1	food					563:566	Test food	558:566	Test food	558:566	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	13	28	theme	potential	1937:1945	arg1	benefit					1947:1953	a potential benefit	1935:1953	a potential benefit of consuming test food	1935:1976	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	6	29	theme	pre-trial	761:769	arg1	food					771:774	pre-trial food	761:774	pre-trial food	761:774	The CKD cats (n = 7) were fed pre-trial food for 28 days and then randomly assigned to control food or test food.
35609046	15	30	theme	one-carbon	2508:2517	arg1	metabolism					2519:2528	one-carbon metabolism	2508:2528	one-carbon metabolism	2508:2528	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	15	31	theme	prebiotics	2409:2418	arg1	addition					2385:2392	the addition	2381:2392	the addition of betaine and prebiotics to the control food formula	2381:2446	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	14	32	dep	EPA	2192:2194	arg1	<					2204:2204	both P < 0.05	2197:2209	both P < 0.05	2197:2209	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	11	33	theme	CKD	1469:1471	arg1	cats					1473:1476	CKD cats	1469:1476	CKD cats	1469:1476	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	5	34	theme	long-chain	640:649	arg1	%					673:673	0.586%	668:673	0.586%	668:673	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	34	theme	long-chain	640:649	arg1	prebiotics					628:637	prebiotics	628:637	prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	628:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	34	theme	long-chain	640:649	arg1	beta-glucan					655:665	long-chain oat beta-glucan	640:665	long-chain oat beta-glucan (0.586%)	640:674	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	9	35	theme	plasma	1252:1257	arg1	metabolites					1259:1269	plasma metabolites	1252:1269	plasma metabolites	1252:1269	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	15	36	from	mass	2478:2481	arg1	cats					2490:2493	CKD cats	2486:2493	CKD cats	2486:2493	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	3	37	from	effects	358:364	arg1	weight					408:413	body weight	403:413	body weight of both CKD and healthy cats	403:442	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	1	38	theme	chronic	150:156	arg1	CKD					174:176	CKD	174:176	CKD	174:176	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	1	38	theme	chronic	150:156	arg1	disease					165:171	chronic kidney disease	150:171	chronic kidney disease (CKD)	150:177	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	11	39	theme	test	1494:1497	arg1	food					1499:1502	test food	1494:1502	test food	1494:1502	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	1	40	with	Cats	140:143	arg1	CKD					174:176	CKD	174:176	CKD	174:176	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	1	40	with	Cats	140:143	arg1	disease					165:171	chronic kidney disease	150:171	chronic kidney disease (CKD)	150:177	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	11	41	theme	=	1630:1630	arg1	P					1628:1628	P = 0.34	1628:1635	P = 0.34	1628:1635	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	11	41	theme	=	1630:1630	arg1	food					1622:1625	control food	1614:1625	control food (P = 0.34)	1614:1636	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	0	42	theme	body	103:106	arg1	mass					108:111	total body mass	97:111	total body mass	97:111	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	3	43	theme	feeding	369:375	arg1	effects					358:364	the combined effects	345:364	the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats	345:442	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	5	44	theme	0.407	680:684	arg1	%					685:685	%	685:685	%	685:685	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	3	45	theme	study	323:327	arg1	goal					310:313	The goal	306:313	The goal of this study	306:327	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	8	46	dep	=	987:987	arg1	group					996:1000	each group	991:1000	8 each group	989:1000	In a second study, healthy cats received control food or test food for 8 weeks (n = 8 each group).
35609046	11	47	from	difference	1564:1573	arg1	intake					1583:1588	food intake	1578:1588	food intake	1578:1588	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	5	48	theme	short	687:691	arg1	scFOS					723:727	scFOS	723:727	scFOS	723:727	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	48	theme	short	687:691	arg1	fructooligosaccharides					699:720	0.407% short chain fructooligosaccharides	680:720	0.407% short chain fructooligosaccharides (scFOS)	680:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	48	theme	short	687:691	arg1	prebiotics					628:637	prebiotics	628:637	prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	628:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	9	49	theme	relevant	1081:1088	arg1	biomarkers					1106:1115	relevant kidney function biomarkers	1081:1115	relevant kidney function biomarkers	1081:1115	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	6	50	theme	CKD	735:737	arg1	n					745:745	n = 7	745:749	n = 7	745:749	The CKD cats (n = 7) were fed pre-trial food for 28 days and then randomly assigned to control food or test food.
35609046	6	50	theme	CKD	735:737	arg1	cats					739:742	The CKD cats	731:742	The CKD cats (n = 7)	731:750	The CKD cats (n = 7) were fed pre-trial food for 28 days and then randomly assigned to control food or test food.
35609046	15	51	theme	betaine	2397:2403	arg1	addition					2385:2392	the addition	2381:2392	the addition of betaine and prebiotics to the control food formula	2381:2446	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	15	52	dep	have	2452:2455	arg1	modulating					2537:2546	modulating	2537:2546	modulating the gut microbiome	2537:2565	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	15	52	dep	have	2452:2455	arg1	enhancing					2498:2506	enhancing	2498:2506	enhancing one-carbon metabolism	2498:2528	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	15	53	theme	food	2435:2438	arg1	formula					2440:2446	the control food formula	2423:2446	the control food formula	2423:2446	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	9	54	theme	CKD	1171:1173	arg1	cats					1175:1178	CKD cats	1171:1178	CKD cats	1171:1178	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	9	55	theme	function	1097:1104	arg1	biomarkers					1106:1115	relevant kidney function biomarkers	1081:1115	relevant kidney function biomarkers	1081:1115	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	0	56	theme	kidney	26:31	arg1	disease					33:39	chronic kidney disease	18:39	chronic kidney disease	18:39	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	11	57	theme	Total	1425:1429	arg1	mass					1436:1439	Total body mass	1425:1439	Total body mass	1425:1439	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	14	58	theme	=	2097:2097	arg1	sulfate					2086:2092	plasma P-cresol sulfate	2070:2092	plasma P-cresol sulfate (P = 0.004)	2070:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	58	theme	=	2097:2097	arg1	0.004					2099:2103	P = 0.004	2095:2103	P = 0.004	2095:2103	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	11	59	theme	significant	1552:1562	arg1	difference					1564:1573	no significant difference	1549:1573	no significant difference in food intake	1549:1588	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	14	60	theme	eicosapentaenoic	2169:2184	arg1	EPA					2192:2194	EPA	2192:2194	EPA	2192:2194	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	60	theme	eicosapentaenoic	2169:2184	arg1	acid					2186:2189	eicosapentaenoic acid	2169:2189	eicosapentaenoic acid (EPA; both P < 0.05)	2169:2210	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	61	theme	acids	2316:2320	arg1	concentrations					2275:2288	similar concentrations	2267:2288	similar concentrations of these long-chain fatty acids	2267:2320	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	4	62	theme	control	465:471	arg1	food					459:462	The pre-trial food	445:462	The pre-trial food (control food)	445:477	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	4	62	theme	control	465:471	arg1	food					473:476	control food	465:476	control food	465:476	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	13	63	theme	function	1914:1921	arg1	level					1898:1902	the level	1894:1902	the level of kidney function	1894:1921	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	10	64	theme	Body	1289:1292	arg1	weight					1294:1299	Body weight	1289:1299	Body weight	1289:1299	Body weight and composition were measured using dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period.
35609046	14	65	theme	P-cresol	2077:2084	arg1	sulfate					2086:2092	plasma P-cresol sulfate	2070:2092	plasma P-cresol sulfate (P = 0.004)	2070:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	65	theme	P-cresol	2077:2084	arg1	0.004					2099:2103	P = 0.004	2095:2103	P = 0.004	2095:2103	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	9	66	theme	fecal	1022:1026	arg1	samples					1028:1034	fecal samples	1022:1034	fecal samples	1022:1034	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	3	67	theme	cats	439:442	arg1	weight					408:413	body weight	403:413	body weight of both CKD and healthy cats	403:442	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	13	68	theme	CKD	1812:1814	arg1	cats					1816:1819	CKD cats	1812:1819	CKD cats fed test food	1812:1833	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	8	69	theme	healthy	924:930	arg1	cats					932:935	healthy cats	924:935	healthy cats	924:935	In a second study, healthy cats received control food or test food for 8 weeks (n = 8 each group).
35609046	14	70	theme	docosahexaenoic	2138:2152	arg1	DHA					2160:2162	DHA	2160:2162	DHA	2160:2162	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	70	theme	docosahexaenoic	2138:2152	arg1	acid					2154:2157	docosahexaenoic acid	2138:2157	docosahexaenoic acid (DHA)	2138:2163	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	71	theme	similar	2267:2273	arg1	concentrations					2275:2288	similar concentrations	2267:2288	similar concentrations of these long-chain fatty acids	2267:2320	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	72	theme	decreased	2042:2050	arg1	concentrations					2052:2065	significantly decreased concentrations	2028:2065	significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004)	2028:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	9	73	theme	period	1160:1165	arg1	end					1140:1142	the end	1136:1142	the end of each feeding period	1136:1165	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	12	74	theme	total	1664:1668	arg1	mass					1675:1678	total body mass	1664:1678	total body mass	1664:1678	Test food did not affect total body mass or composition of healthy cats.
35609046	6	75	theme	=	747:747	arg1	n					745:745	n = 7	745:749	n = 7	745:749	The CKD cats (n = 7) were fed pre-trial food for 28 days and then randomly assigned to control food or test food.
35609046	6	75	theme	=	747:747	arg1	cats					739:742	The CKD cats	731:742	The CKD cats (n = 7)	731:750	The CKD cats (n = 7) were fed pre-trial food for 28 days and then randomly assigned to control food or test food.
35609046	4	76	theme	dry	507:509	arg1	food					459:462	The pre-trial food	445:462	The pre-trial food (control food)	445:477	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	4	76	theme	dry	507:509	arg1	food					511:514	a complete and balanced dry food	483:514	a complete and balanced dry food designed to aid in the management of CKD	483:555	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	7	77	theme	study	891:895	arg1	design					897:902	a cross-over study design	878:902	a cross-over study design	878:902	Each food was fed for 8 weeks in a cross-over study design.
35609046	1	78	theme	kidney	158:163	arg1	CKD					174:176	CKD	174:176	CKD	174:176	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	1	78	theme	kidney	158:163	arg1	disease					165:171	chronic kidney disease	150:171	chronic kidney disease (CKD)	150:177	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	10	79	theme	X-ray	1349:1353	arg1	DEXA					1371:1374	DEXA	1371:1374	DEXA	1371:1374	Body weight and composition were measured using dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period.
35609046	10	79	theme	X-ray	1349:1353	arg1	absorptiometry					1355:1368	dual-energy X-ray absorptiometry	1337:1368	dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period	1337:1422	Body weight and composition were measured using dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period.
35609046	8	80	theme	test	962:965	arg1	food					967:970	control food or test food	946:970	food	967:970	In a second study, healthy cats received control food or test food for 8 weeks (n = 8 each group).
35609046	15	81	theme	body	2473:2476	arg1	mass					2478:2481	increased total body mass	2457:2481	increased total body mass in CKD cats	2457:2493	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	11	82	theme	P	1532:1532	arg1	0.004					1536:1540	P = 0.004	1532:1540	P = 0.004	1532:1540	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	11	82	theme	P	1532:1532	arg1	food					1526:1529	control food	1518:1529	control food (P = 0.004)	1518:1541	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	14	83	theme	consuming	1996:2004	arg1	food					2011:2014	consuming test food	1996:2014	consuming test food	1996:2014	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	2	84	theme	weight	259:264	arg1	loss					246:249	loss	246:249	loss of body weight	246:264	As CKD advances, loss of body weight contributes to morbidity and mortality.
35609046	5	85	theme	chain	693:697	arg1	scFOS					723:727	scFOS	723:727	scFOS	723:727	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	85	theme	chain	693:697	arg1	fructooligosaccharides					699:720	0.407% short chain fructooligosaccharides	680:720	0.407% short chain fructooligosaccharides (scFOS)	680:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	85	theme	chain	693:697	arg1	prebiotics					628:637	prebiotics	628:637	prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	628:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	14	86	theme	test	2252:2255	arg1	foods					2257:2261	both control and test foods	2235:2261	foods	2257:2261	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	87	theme	P	2095:2095	arg1	sulfate					2086:2092	plasma P-cresol sulfate	2070:2092	plasma P-cresol sulfate (P = 0.004)	2070:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	87	theme	P	2095:2095	arg1	0.004					2099:2103	P = 0.004	2095:2103	P = 0.004	2095:2103	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	15	88	theme	increased	2457:2465	arg1	mass					2478:2481	increased total body mass	2457:2481	increased total body mass in CKD cats	2457:2493	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	15	89	theme	control	2427:2433	arg1	formula					2440:2446	the control food formula	2423:2446	the control food formula	2423:2446	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	13	90	theme	food	1973:1976	arg1	benefit					1947:1953	a potential benefit	1935:1953	a potential benefit of consuming test food	1935:1976	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	14	91	theme	control	2240:2246	arg1	foods					2257:2261	both control and test foods	2235:2261	foods	2257:2261	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	13	92	theme	cats	1816:1819	arg1	feces					1803:1807	feces	1803:1807	feces of CKD cats fed test food	1803:1833	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	5	93	dep	prebiotics	628:637	arg1	%					673:673	0.586%	668:673	0.586%	668:673	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	93	dep	prebiotics	628:637	arg1	scFOS					723:727	scFOS	723:727	scFOS	723:727	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	93	dep	prebiotics	628:637	arg1	fructooligosaccharides					699:720	0.407% short chain fructooligosaccharides	680:720	0.407% short chain fructooligosaccharides (scFOS)	680:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	93	dep	prebiotics	628:637	arg1	prebiotics					628:637	prebiotics	628:637	prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	628:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	93	dep	prebiotics	628:637	arg1	beta-glucan					655:665	long-chain oat beta-glucan	640:665	long-chain oat beta-glucan (0.586%)	640:674	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	2	94	theme	CKD	232:234	arg1	advances					236:243	CKD advances	232:243	CKD advances	232:243	As CKD advances, loss of body weight contributes to morbidity and mortality.
35609046	9	95	theme	healthy	1275:1281	arg1	cats					1283:1286	healthy cats	1275:1286	healthy cats	1275:1286	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	14	96	contain	had	2263:2265	arg1	foods					2257:2261	both control and test foods	2235:2261	foods	2257:2261	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	96	contain	had	2263:2265	arg2	concentrations					2275:2288	similar concentrations	2267:2288	similar concentrations of these long-chain fatty acids	2267:2320	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	97	theme	healthy	1982:1988	arg1	cats					1990:1993	healthy cats	1982:1993	healthy cats	1982:1993	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	9	98	theme	blood	1185:1189	arg1	samples					1191:1197	blood samples	1185:1197	blood samples	1185:1197	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	13	99	theme	kidney	1907:1912	arg1	function					1914:1921	kidney function	1907:1921	kidney function	1907:1921	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	5	100	theme	oat	651:653	arg1	%					673:673	0.586%	668:673	0.586%	668:673	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	100	theme	oat	651:653	arg1	prebiotics					628:637	prebiotics	628:637	prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS)	628:728	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	5	100	theme	oat	651:653	arg1	beta-glucan					655:665	long-chain oat beta-glucan	640:665	long-chain oat beta-glucan (0.586%)	640:674	Test food was the control food supplemented with betaine (0.500%) and prebiotics: long-chain oat beta-glucan (0.586%) and 0.407% short chain fructooligosaccharides (scFOS).
35609046	13	101	theme	bacterial	1741:1749	arg1	metabolism					1751:1760	bacterial metabolism	1741:1760	bacterial metabolism	1741:1760	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	9	102	theme	metabolites	1259:1269	arg1	concentrations					1234:1247	concentrations	1234:1247	concentrations of plasma metabolites	1234:1269	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	4	103	theme	pre-trial	449:457	arg1	food					511:514	a complete and balanced dry food	483:514	a complete and balanced dry food designed to aid in the management of CKD	483:555	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	4	103	theme	pre-trial	449:457	arg1	food					459:462	The pre-trial food	445:462	The pre-trial food (control food)	445:477	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	4	103	theme	pre-trial	449:457	arg1	food					473:476	control food	465:476	control food	465:476	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	0	104	theme	total	97:101	arg1	mass					108:111	total body mass	97:111	total body mass	97:111	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	3	105	dep	both	418:421	arg1	CKD					423:425	CKD	423:425	CKD	423:425	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	13	106	theme	consuming	1958:1966	arg1	food					1973:1976	consuming test food	1958:1976	consuming test food	1958:1976	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	14	107	theme	P	2202:2202	arg1	<					2204:2204	both P < 0.05	2197:2209	both P < 0.05	2197:2209	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	108	theme	long-chain	2299:2308	arg1	acids					2316:2320	these long-chain fatty acids	2293:2320	these long-chain fatty acids	2293:2320	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	13	109	theme	Indole	1712:1717	arg1	compounds					1719:1727	Indole compounds	1712:1727	Indole compounds produced by bacterial metabolism	1712:1760	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	3	110	theme	prebiotics	389:398	arg1	effects					358:364	the combined effects	345:364	the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats	345:442	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	0	111	theme	cats	8:11	arg1	food					41:44	Feeding cats with chronic kidney disease food	0:44	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics	0:85	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	3	112	dep	cats	439:442	arg1	both					418:421	both	418:421	both	418:421	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	3	112	dep	cats	439:442	arg1	healthy					431:437	healthy	431:437	healthy	431:437	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	0	113	theme	chronic	18:24	arg1	disease					33:39	chronic kidney disease	18:39	chronic kidney disease	18:39	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	15	114	contain	have	2452:2455	arg1	addition					2385:2392	the addition	2381:2392	the addition of betaine and prebiotics to the control food formula	2381:2446	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	15	114	contain	have	2452:2455	arg2	mass					2478:2481	increased total body mass	2457:2481	increased total body mass in CKD cats	2457:2493	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	4	115	theme	complete	485:492	arg1	food					459:462	The pre-trial food	445:462	The pre-trial food (control food)	445:477	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	4	115	theme	complete	485:492	arg1	food					511:514	a complete and balanced dry food	483:514	a complete and balanced dry food designed to aid in the management of CKD	483:555	The pre-trial food (control food) was a complete and balanced dry food designed to aid in the management of CKD.
35609046	11	116	theme	body	1431:1434	arg1	mass					1436:1439	Total body mass	1425:1439	Total body mass	1425:1439	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	10	117	theme	feeding	1409:1415	arg1	period					1417:1422	each feeding period	1404:1422	each feeding period	1404:1422	Body weight and composition were measured using dual-energy X-ray absorptiometry (DEXA) scan at baseline and after each feeding period.
35609046	9	118	theme	kidney	1090:1095	arg1	biomarkers					1106:1115	relevant kidney function biomarkers	1081:1115	relevant kidney function biomarkers	1081:1115	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	3	119	dep	feeding	369:375	arg1	betaine					377:383	betaine	377:383	betaine	377:383	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	15	120	theme	gut	2552:2554	arg1	microbiome					2556:2565	the gut microbiome	2548:2565	the gut microbiome	2548:2565	These results suggest that the addition of betaine and prebiotics to the control food formula may have increased total body mass in CKD cats by enhancing one-carbon metabolism and by modulating the gut microbiome.
35609046	8	121	dep	weeks	978:982	arg1	n					985:985	n	985:985	n = 8 each group	985:1000	In a second study, healthy cats received control food or test food for 8 weeks (n = 8 each group).
35609046	3	122	theme	body	403:406	arg1	weight					408:413	body weight	403:413	body weight of both CKD and healthy cats	403:442	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	9	123	theme	biomarkers	1106:1115	arg1	concentrations					1063:1076	concentrations	1063:1076	concentrations of relevant kidney function biomarkers and metabolites	1063:1131	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	14	124	theme	acid	2186:2189	arg1	concentrations					2120:2133	increased concentrations	2110:2133	increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05)	2110:2210	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	124	theme	acid	2186:2189	arg1	concentrations					2052:2065	significantly decreased concentrations	2028:2065	significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004)	2028:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	3	125	theme	combined	349:356	arg1	effects					358:364	the combined effects	345:364	the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats	345:442	The goal of this study was to evaluate the combined effects of feeding betaine and prebiotics on body weight of both CKD and healthy cats.
35609046	0	126	with	cats	8:11	arg1	disease					33:39	chronic kidney disease	18:39	chronic kidney disease	18:39	Feeding cats with chronic kidney disease food supplemented with betaine and prebiotics increases total body mass and reduces uremic toxins.
35609046	9	127	theme	metabolites	1121:1131	arg1	concentrations					1063:1076	concentrations	1063:1076	concentrations of relevant kidney function biomarkers and metabolites	1063:1131	Blood, urine, and fecal samples were collected to evaluate concentrations of relevant kidney function biomarkers and metabolites at the end of each feeding period for CKD cats, and blood samples were collected monthly to evaluate concentrations of plasma metabolites for healthy cats.
35609046	8	128	theme	second	910:915	arg1	study					917:921	a second study	908:921	a second study	908:921	In a second study, healthy cats received control food or test food for 8 weeks (n = 8 each group).
35609046	14	129	theme	fatty	2310:2314	arg1	acids					2316:2320	these long-chain fatty acids	2293:2320	these long-chain fatty acids	2293:2320	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	1	130	contain	have	179:182	arg1	Cats					140:143	Cats	140:143	Cats with chronic kidney disease (CKD)	140:177	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	1	130	contain	have	179:182	arg2	ability					196:202	a decreased ability	184:202	a decreased ability to maintain body weight	184:226	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35609046	14	131	theme	sulfate	2086:2092	arg1	concentrations					2120:2133	increased concentrations	2110:2133	increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05)	2110:2210	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	131	theme	sulfate	2086:2092	arg1	concentrations					2052:2065	significantly decreased concentrations	2028:2065	significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004)	2028:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	11	132	theme	control	1614:1620	arg1	P					1628:1628	P = 0.34	1628:1635	P = 0.34	1628:1635	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	11	132	theme	control	1614:1620	arg1	food					1622:1625	control food	1614:1625	control food (P = 0.34)	1614:1636	Total body mass was significantly higher in CKD cats after consuming test food compared with control food (P = 0.004), with no significant difference in food intake while consuming test or control food (P = 0.34).
35609046	8	133	theme	=	987:987	arg1	n					985:985	n	985:985	n = 8 each group	985:1000	In a second study, healthy cats received control food or test food for 8 weeks (n = 8 each group).
35609046	13	134	theme	plasma	1840:1845	arg1	concentrations					1847:1860	plasma concentrations	1840:1860	plasma concentrations	1840:1860	Indole compounds produced by bacterial metabolism were decreased in urine and increased in feces of CKD cats fed test food, and plasma concentrations were negatively correlated with the level of kidney function, indicating a potential benefit of consuming test food.
35609046	14	135	theme	plasma	2070:2075	arg1	sulfate					2086:2092	plasma P-cresol sulfate	2070:2092	plasma P-cresol sulfate (P = 0.004)	2070:2104	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	14	135	theme	plasma	2070:2075	arg1	0.004					2099:2103	P = 0.004	2095:2103	P = 0.004	2095:2103	In healthy cats, consuming test food resulted in significantly decreased concentrations of plasma P-cresol sulfate (P = 0.004) and increased concentrations of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA; both P < 0.05), despite the fact that both control and test foods had similar concentrations of these long-chain fatty acids, 0.03% and 0.02%, respectively.
35609046	1	136	theme	body	216:219	arg1	weight					221:226	body weight	216:226	body weight	216:226	Cats with chronic kidney disease (CKD) have a decreased ability to maintain body weight.
35018700	2	0	theme	age	451:453	arg1	6					432:432	6	432:432	6	432:432	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	2	0	theme	age	451:453	arg1	age					451:453	age	451:453	age	451:453	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	5	1	theme	Greater	1072:1078	arg1	inheritance					1089:1099	Greater maternal inheritance	1072:1099	Greater maternal inheritance of the faecal microbiome	1072:1124	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	1	2	theme	koalas	141:146	arg1	microbiomes					116:126	the faecal microbiomes	105:126	the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers	105:215	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	4	3	theme	cellulose	928:936	arg1	degradation					938:948	cellulose degradation	928:948	cellulose degradation	928:948	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	5	4	theme	maternal	1080:1087	arg1	inheritance					1089:1099	Greater maternal inheritance	1072:1099	Greater maternal inheritance of the faecal microbiome	1072:1124	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	4	5	theme	fibre-degrading	803:817	arg1	genes					819:823	the fibre-degrading genes	799:823	the fibre-degrading genes that increased in abundance over the development of captive joeys	799:889	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	4	6	theme	higher	1008:1013	arg1	abundance					1015:1023	higher abundance	1008:1023	higher abundance in adult wild koalas	1008:1044	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	2	7	theme	9-month-old	478:488	arg1	joeys					490:494	9-month-old joeys	478:494	9-month-old joeys	478:494	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	5	8	theme	captive	1151:1157	arg1	koalas					1159:1164	captive koalas	1151:1164	captive koalas	1151:1164	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	5	9	from	wild	1138:1141	arg1	koalas					1159:1164	captive koalas	1151:1164	captive koalas	1151:1164	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	1	10	theme	gut	239:241	arg1	maturation					254:263	gut microbiome maturation	239:263	gut microbiome maturation	239:263	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	2	11	dep	similar	506:512	arg1	microbiomes					463:473	the microbiomes	459:473	the microbiomes of 9-month-old joeys	459:494	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	2	11	dep	similar	506:512	arg1	similar					506:512	similar	506:512	similar	506:512	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	2	11	dep	similar	506:512	arg1	distinct					571:578	distinct	571:578	distinct	571:578	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	6	12	from	development	1305:1315	arg1	composition					1356:1366	microbiome composition	1345:1366	microbiome composition	1345:1366	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	12	from	development	1305:1315	arg1	health					1380:1385	population health	1369:1385	population health	1369:1385	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	12	from	development	1305:1315	arg1	diet					1391:1394	diet	1391:1394	diet	1391:1394	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	4	13	theme	wild	1034:1037	arg1	koalas					1039:1044	adult wild koalas	1028:1044	adult wild koalas	1028:1044	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	2	14	theme	joey	360:363	arg1	koala					365:369	joey koala	360:369	joey koala	360:369	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	1	15	from	inheritance	269:279	arg1	wild					288:291	wild	288:291	wild	288:291	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	5	16	theme	wild	1213:1216	arg1	koalas					1218:1223	wild koalas	1213:1223	wild koalas	1213:1223	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	5	17	theme	solitary	1194:1201	arg1	nature					1203:1208	the more solitary nature	1185:1208	the more solitary nature of wild koalas	1185:1223	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	6	18	theme	differences	1330:1340	arg1	development					1305:1315	the development	1301:1315	the development of localized differences in microbiome composition, population health and diet	1301:1394	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	19	theme	spatial	1404:1410	arg1	clustering					1412:1421	spatial clustering	1404:1421	spatial clustering of relatives	1404:1434	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	20	theme	microbiome	1345:1354	arg1	composition					1356:1366	microbiome composition	1345:1366	microbiome composition	1345:1366	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	2	21	theme	adult	407:411	arg1	assemblage					413:422	an adult assemblage	404:422	an adult assemblage	404:422	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	1	22	theme	microbiome	243:252	arg1	maturation					254:263	gut microbiome maturation	239:263	gut microbiome maturation	239:263	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	1	23	from	maturation	254:263	arg1	wild					288:291	wild	288:291	wild	288:291	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	5	24	from	koalas	1159:1164	arg1	wild					1138:1141	wild	1138:1141	wild	1138:1141	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	0	25	theme	juvenile	24:31	arg1	gut					39:41	the juvenile koala gut	20:41	the juvenile koala gut	20:41	Characterization of the juvenile koala gut microbiome across wild populations.
35018700	6	26	theme	microbiome	1270:1279	arg1	inheritance					1247:1257	This strong maternal inheritance	1226:1257	This strong maternal inheritance of the gut microbiome	1226:1279	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	27	from	differences	1330:1340	arg1	composition					1356:1366	microbiome composition	1345:1366	microbiome composition	1345:1366	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	27	from	differences	1330:1340	arg1	health					1380:1385	population health	1369:1385	population health	1369:1385	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	27	from	differences	1330:1340	arg1	diet					1391:1394	diet	1391:1394	diet	1391:1394	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	6	28	theme	population	1369:1378	arg1	health					1380:1385	population health	1369:1385	population health	1369:1385	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	5	29	theme	koalas	1218:1223	arg1	nature					1203:1208	the more solitary nature	1185:1208	the more solitary nature of wild koalas	1185:1223	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	6	30	from	health	1380:1385	arg1	development					1305:1315	the development	1301:1315	the development of localized differences in microbiome composition, population health and diet	1301:1394	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	0	31	theme	gut	39:41	arg1	Characterization					0:15	Characterization	0:15	Characterization of the juvenile koala gut	0:41	Characterization of the juvenile koala gut microbiome across wild populations.
35018700	6	32	theme	gut	1266:1268	arg1	microbiome					1270:1279	the gut microbiome	1262:1279	the gut microbiome	1262:1279	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	4	33	theme	joeys	885:889	arg1	development					862:872	the development	858:872	the development of captive joeys	858:889	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	3	34	theme	phylum	588:593	arg1	level					595:599	the phylum level	584:599	the phylum level	584:599	At the phylum level, differences between joeys and adults were broadly consistent with those in captivity, with Firmicutes increasing in relative abundance over the joeys' development and Proteobacteria decreasing.
35018700	6	35	theme	relatives	1426:1434	arg1	clustering					1412:1421	spatial clustering	1404:1421	spatial clustering of relatives	1404:1434	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	0	36	theme	koala	33:37	arg1	gut					39:41	the juvenile koala gut	20:41	the juvenile koala gut	20:41	Characterization of the juvenile koala gut microbiome across wild populations.
35018700	2	37	theme	joeys	490:494	arg1	microbiomes					463:473	the microbiomes	459:473	the microbiomes of 9-month-old joeys	459:494	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	2	37	theme	joeys	490:494	arg1	similar					506:512	similar	506:512	similar	506:512	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	2	37	theme	joeys	490:494	arg1	distinct					571:578	distinct	571:578	distinct	571:578	Our findings suggest that joey koala microbiomes slowly shift towards an adult assemblage between 6 and 12 months of age, as the microbiomes of 9-month-old joeys were more similar to those of adults than those of 7-month-olds, but still distinct.
35018700	6	38	from	diet	1391:1394	arg1	development					1305:1315	the development	1301:1315	the development of localized differences in microbiome composition, population health and diet	1301:1394	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	4	39	theme	pectin	959:964	arg1	degradation					966:976	pectin degradation	959:976	pectin degradation	959:976	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	3	40	theme	relative	718:725	arg1	abundance					727:735	relative abundance	718:735	relative abundance	718:735	At the phylum level, differences between joeys and adults were broadly consistent with those in captivity, with Firmicutes increasing in relative abundance over the joeys' development and Proteobacteria decreasing.
35018700	6	41	theme	localized	1320:1328	arg1	differences					1330:1340	localized differences	1320:1340	localized differences in microbiome composition, population health and diet	1320:1394	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	1	42	dep	Phascolarctos	149:161	arg1	cinereus					163:170	Phascolarctos cinereus	149:170	Phascolarctos cinereus	149:170	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	4	43	theme	captive	877:883	arg1	joeys					885:889	captive joeys	877:889	captive joeys	877:889	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	5	44	theme	faecal	1108:1113	arg1	microbiome					1115:1124	the faecal microbiome	1104:1124	the faecal microbiome	1104:1124	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	6	45	theme	maternal	1238:1245	arg1	inheritance					1247:1257	This strong maternal inheritance	1226:1257	This strong maternal inheritance of the gut microbiome	1226:1279	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	4	46	located	found	999:1003	arg2	those					892:896	those	892:896	those	892:896	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	4	46	located	found	999:1003	arg1	abundance					1015:1023	higher abundance	1008:1023	higher abundance in adult wild koalas	1008:1044	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	1	47	theme	faecal	109:114	arg1	microbiomes					116:126	the faecal microbiomes	105:126	the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers	105:215	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	5	48	theme	microbiome	1115:1124	arg1	inheritance					1089:1099	Greater maternal inheritance	1072:1099	Greater maternal inheritance of the faecal microbiome	1072:1124	Greater maternal inheritance of the faecal microbiome was seen in wild than in captive koalas, presumably due to the more solitary nature of wild koalas.
35018700	6	49	theme	strong	1231:1236	arg1	inheritance					1247:1257	This strong maternal inheritance	1226:1257	This strong maternal inheritance of the gut microbiome	1226:1279	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	3	50	with	consistent	652:661	arg1	those					668:672	those	668:672	those	668:672	At the phylum level, differences between joeys and adults were broadly consistent with those in captivity, with Firmicutes increasing in relative abundance over the joeys' development and Proteobacteria decreasing.
35018700	4	51	from	abundance	1015:1023	arg1	koalas					1039:1044	adult wild koalas	1028:1044	adult wild koalas	1028:1044	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	3	52	theme	decreasing	784:793	arg1	Proteobacteria					769:782	Proteobacteria decreasing	769:793	Proteobacteria decreasing	769:793	At the phylum level, differences between joeys and adults were broadly consistent with those in captivity, with Firmicutes increasing in relative abundance over the joeys' development and Proteobacteria decreasing.
35018700	3	53	from	level	595:599	arg1	consistent					652:661	consistent	652:661	consistent	652:661	At the phylum level, differences between joeys and adults were broadly consistent with those in captivity, with Firmicutes increasing in relative abundance over the joeys' development and Proteobacteria decreasing.
35018700	6	54	from	composition	1356:1366	arg1	development					1305:1315	the development	1301:1315	the development of localized differences in microbiome composition, population health and diet	1301:1394	This strong maternal inheritance of the gut microbiome could contribute to the development of localized differences in microbiome composition, population health and diet through spatial clustering of relatives.
35018700	1	55	theme	wild	131:134	arg1	mothers					209:215	their mothers	203:215	their mothers	203:215	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	1	55	theme	wild	131:134	arg1	Phascolarctos					149:161	Phascolarctos	149:161	Phascolarctos	149:161	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	1	55	theme	wild	131:134	arg1	koalas					141:146	wild joey koalas	131:146	wild joey koalas (Phascolarctos cinereus)	131:171	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	4	56	theme	adult	1028:1032	arg1	koalas					1039:1044	adult wild koalas	1028:1044	adult wild koalas	1028:1044	Of the fibre-degrading genes that increased in abundance over the development of captive joeys, those involved in hemicellulose and cellulose degradation, but not pectin degradation, were also generally found in higher abundance in adult wild koalas compared to 7-month-olds.
35018700	0	57	theme	wild	61:64	arg1	populations					66:76	wild populations	61:76	wild populations	61:76	Characterization of the juvenile koala gut microbiome across wild populations.
35018700	1	58	theme	joey	136:139	arg1	mothers					209:215	their mothers	203:215	their mothers	203:215	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	1	58	theme	joey	136:139	arg1	Phascolarctos					149:161	Phascolarctos	149:161	Phascolarctos	149:161	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
35018700	1	58	theme	joey	136:139	arg1	koalas					141:146	wild joey koalas	131:146	wild joey koalas (Phascolarctos cinereus)	131:171	In this study we compared the faecal microbiomes of wild joey koalas (Phascolarctos cinereus) to those of adults, including their mothers, to establish whether gut microbiome maturation and inheritance in the wild is comparable to that seen in captivity.
37367683	8	0	from	minerals	1284:1291	arg1	calcium					1259:1265	The calcium	1255:1265	The calcium from the lobster minerals	1255:1291	The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g).
37367683	8	0	from	minerals	1284:1291	arg1	comparable					1309:1318	comparable	1309:1318	comparable	1309:1318	The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g).
37367683	4	1	theme	lobster	677:683	arg1	shells					685:690	lobster shells	677:690	lobster shells	677:690	However, we recently developed a microwave-intensified biorefinery that efficiently produced chitin, proteins/peptides, and minerals from lobster shells.
37367683	7	2	theme	macrophage	1229:1238	arg1	cells					1248:1252	macrophage (THP-1) cells	1229:1252	macrophage (THP-1) cells	1229:1252	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	17	3	theme	calcium	3065:3071	arg1	minerals					2999:3006	lobster minerals	2991:3006	lobster minerals	2991:3006	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	17	3	theme	calcium	3065:3071	arg1	source					3027:3032	a source	3025:3032	a source of functional or nutraceutical calcium for commercial products	3025:3095	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	15	4	theme	MG-63	2555:2559	arg1	cells					2571:2575	the MG-63 and HaCaT cells	2551:2575	cells	2571:2575	Furthermore, the MG-63 and HaCaT cells proliferated considerably after seven days of incubation, reaching 100.3% for MG-63 and 115.9% for HaCaT with a lobster mineral supplementation of 15%.
37367683	10	5	theme	1.99	1965:1968	arg1	%					1969:1969	11.95 vs. 1.99%	1955:1969	%	1969:1969	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	5	theme	1.99	1965:1968	arg1	product					1946:1952	the commercial product	1931:1952	the commercial product (11.95 vs. 1.99%)	1931:1970	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	11	6	from	ratios	2029:2034	arg1	media					2020:2024	media	2020:2024	media at ratios of 15%, 25%, and 35% (v/v) when growing cells	2020:2080	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	16	7	theme	cytotoxicity	2928:2939	arg1	threshold					2941:2949	the cytotoxicity threshold	2924:2949	the cytotoxicity threshold (<70%)	2924:2956	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	16	7	theme	cytotoxicity	2928:2939	arg1	%					2955:2955	<70%	2952:2955	<70%	2952:2955	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	6	8	theme	further	912:918	arg1	investigation					920:932	a further investigation	910:932	a further investigation of lobster minerals for commercial applications	910:980	This has suggested a further investigation of lobster minerals for commercial applications.
37367683	10	9	dep	CCS	1763:1765	arg1	%					1781:1781	98.4 vs. 18.6%	1768:1781	98.4 vs. 18.6% for the products	1768:1798	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	9	dep	CCS	1763:1765	arg1	%					1816:1816	64.0 vs. 8.5%	1804:1816	64.0 vs. 8.5% for their calcium	1804:1834	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	12	10	theme	significant	2170:2180	arg1	effects					2182:2188	significant effects	2170:2188	significant effects	2170:2188	However, it had significant effects on cell growth and proliferation.
37367683	0	11	theme	High	0:3	arg1	Solubility					5:14	High Solubility	0:14	High Solubility	0:14	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	8	12	theme	lobster	1276:1282	arg1	minerals					1284:1291	the lobster minerals	1272:1291	the lobster minerals	1272:1291	The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g).
37367683	13	13	theme	culture	2267:2273	arg1	days					2259:2262	three days	2253:2262	three days of culture supplemented with the lobster minerals	2253:2312	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	13	14	theme	CCS	2331:2333	arg1	supplementation					2335:2349	the CCS supplementation	2327:2349	the CCS supplementation	2327:2349	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	16	15	theme	mg/mL	2825:2829	arg1	concentrations					2797:2810	concentrations	2797:2810	concentrations of 1.24-2.89 mg/mL	2797:2829	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	1	16	theme	shellfish	192:200	arg1	industry					202:209	the shellfish industry	188:209	the shellfish industry	188:209	Shell wastes pose environmental and financial burdens to the shellfish industry.
37367683	16	17	contain	had	2831:2833	arg1	Macrophages					2729:2739	Macrophages	2729:2739	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL	2729:2829	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	16	17	contain	had	2831:2833	arg1	cells					2748:2752	THP-1 cells	2742:2752	THP-1 cells	2742:2752	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	16	17	contain	had	2831:2833	arg2	changes					2849:2855	no detectable changes	2835:2855	no detectable changes	2835:2855	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	15	18	theme	incubation	2623:2632	arg1	days					2615:2618	seven days	2609:2618	seven days of incubation	2609:2632	Furthermore, the MG-63 and HaCaT cells proliferated considerably after seven days of incubation, reaching 100.3% for MG-63 and 115.9% for HaCaT with a lobster mineral supplementation of 15%.
37367683	8	19	theme	calcium	1344:1350	arg1	supplement					1352:1361	a commercial calcium supplement	1331:1361	a commercial calcium supplement (CCS, 139 vs. 148 mg/g)	1331:1385	The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g).
37367683	9	20	contain	had	1580:1582	arg2	capacity					1618:1625	a considerably higher oil binding capacity	1584:1625	a considerably higher oil binding capacity	1584:1625	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	9	20	contain	had	1580:1582	arg1	mineral					1572:1578	mineral	1572:1578	mineral	1572:1578	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	10	21	theme	in	1847:1848	arg1	5.9-fold					1895:1902	5.9-fold	1895:1902	5.9-fold	1895:1902	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	21	theme	in	1847:1848	arg1	bioavailability					1856:1870	the in vitro bioavailability	1843:1870	the in vitro bioavailability of lobster calcium	1843:1889	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	21	theme	in	1847:1848	arg1	higher					1904:1909	higher	1904:1909	higher	1904:1909	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	5	22	theme	functional	813:822	arg1	ingredient					851:860	a functional, dietary, or nutraceutical ingredient	811:860	a functional, dietary, or nutraceutical ingredient in many commercial products	811:888	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	15	23	theme	lobster	2689:2695	arg1	supplementation					2705:2719	a lobster mineral supplementation	2687:2719	a lobster mineral supplementation of 15%	2687:2726	Furthermore, the MG-63 and HaCaT cells proliferated considerably after seven days of incubation, reaching 100.3% for MG-63 and 115.9% for HaCaT with a lobster mineral supplementation of 15%.
37367683	11	24	theme	detectable	2101:2110	arg1	changes					2112:2118	any detectable changes	2097:2118	any detectable changes	2097:2118	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	5	25	theme	dietary	825:831	arg1	ingredient					851:860	a functional, dietary, or nutraceutical ingredient	811:860	a functional, dietary, or nutraceutical ingredient in many commercial products	811:888	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	7	26	theme	simulated	1138:1146	arg1	digestion					1165:1173	in vitro simulated gastrointestinal digestion	1129:1173	in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells	1129:1252	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	9	27	theme	calcium	1508:1514	arg1	lactate					1516:1522	commercial calcium lactate	1497:1522	commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%)	1497:1553	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	10	28	theme	lobster	1875:1881	arg1	calcium					1883:1889	lobster calcium	1875:1889	lobster calcium	1875:1889	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	7	29	theme	growing	1189:1195	arg1	MG-63					1203:1207	MG-63	1203:1207	MG-63	1203:1207	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	7	29	theme	growing	1189:1195	arg1	bone					1197:1200	growing bone	1189:1200	growing bone (MG-63)	1189:1208	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	2	30	theme	undervalued	228:238	arg1	shells					240:245	these undervalued shells	222:245	these undervalued shells	222:245	Utilizing these undervalued shells for commercial chitin production could minimize their adverse impacts while maximizing economic value.
37367683	7	31	theme	in	1129:1130	arg1	digestion					1165:1173	in vitro simulated gastrointestinal digestion	1129:1173	in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells	1129:1252	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	10	32	dep	5.9-fold	1895:1902	arg1	5.9-fold					1895:1902	5.9-fold	1895:1902	5.9-fold	1895:1902	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	32	dep	5.9-fold	1895:1902	arg1	bioavailability					1856:1870	the in vitro bioavailability	1843:1870	the in vitro bioavailability of lobster calcium	1843:1889	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	32	dep	5.9-fold	1895:1902	arg1	higher					1904:1909	higher	1904:1909	higher	1904:1909	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	11	33	theme	%	2055:2055	arg1	ratios					2029:2034	ratios	2029:2034	ratios of 15%, 25%, and 35% (v/v) when growing cells	2029:2080	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	5	34	theme	many	865:868	arg1	products					881:888	many commercial products	865:888	many commercial products	865:888	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	2	35	theme	chitin	262:267	arg1	production					269:278	commercial chitin production	251:278	commercial chitin production	251:278	Utilizing these undervalued shells for commercial chitin production could minimize their adverse impacts while maximizing economic value.
37367683	7	36	theme	minerals	1100:1107	arg1	effects					1063:1069	nutraceutical effects	1049:1069	nutraceutical effects	1049:1069	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	7	36	theme	minerals	1100:1107	arg1	cytotoxicity					1076:1087	cytotoxicity	1076:1087	cytotoxicity of lobster minerals	1076:1107	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	7	36	theme	minerals	1100:1107	arg1	attributes					1014:1023	the nutritional attributes	998:1023	the nutritional attributes	998:1023	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	7	36	theme	minerals	1100:1107	arg1	properties					1037:1046	functional properties	1026:1046	functional properties	1026:1046	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	1	37	theme	environmental	149:161	arg1	burdens					177:183	environmental and financial burdens	149:183	environmental and financial burdens	149:183	Shell wastes pose environmental and financial burdens to the shellfish industry.
37367683	3	38	theme	Shell	350:354	arg1	chitin					356:361	Shell chitin	350:361	Shell chitin conventionally produced through harsh chemical processes	350:418	Shell chitin conventionally produced through harsh chemical processes is environmentally unfriendly and infeasible for recovering compatible proteins and minerals for value-added products.
37367683	17	39	theme	functional	3037:3046	arg1	calcium					3065:3071	functional or nutraceutical calcium	3037:3071	functional or nutraceutical calcium	3037:3071	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	10	40	theme	commercial	1935:1944	arg1	%					1969:1969	11.95 vs. 1.99%	1955:1969	%	1969:1969	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	40	theme	commercial	1935:1944	arg1	product					1946:1952	the commercial product	1931:1952	the commercial product (11.95 vs. 1.99%)	1931:1970	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	0	41	theme	Proliferating	87:99	arg1	Cells					115:119	Significantly Proliferating Bone and Skin Cells	73:119	Significantly Proliferating Bone and Skin Cells	73:119	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	5	42	theme	Lobster	693:699	arg1	minerals					701:708	Lobster minerals	693:708	Lobster minerals	693:708	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	1	43	theme	financial	167:175	arg1	burdens					177:183	environmental and financial burdens	149:183	environmental and financial burdens	149:183	Shell wastes pose environmental and financial burdens to the shellfish industry.
37367683	3	44	theme	chemical	401:408	arg1	processes					410:418	harsh chemical processes	395:418	harsh chemical processes	395:418	Shell chitin conventionally produced through harsh chemical processes is environmentally unfriendly and infeasible for recovering compatible proteins and minerals for value-added products.
37367683	17	45	theme	commercial	3077:3086	arg1	products					3088:3095	commercial products	3077:3095	commercial products	3077:3095	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	9	46	dep	CCL	1525:1527	arg1	15.1					1539:1542	15.1	1539:1542	15.1	1539:1542	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	9	46	dep	CCL	1525:1527	arg1	%					1552:1552	21.1 vs. 15.1 and 13.3%	1530:1552	%	1552:1552	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	9	46	dep	CCL	1525:1527	arg1	21.1					1530:1533	21.1	1530:1533	21.1	1530:1533	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	17	47	theme	nutraceutical	3051:3063	arg1	calcium					3065:3071	functional or nutraceutical calcium	3037:3071	functional or nutraceutical calcium	3037:3071	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	4	48	from	shells	685:690	arg1	chitin					632:637	chitin	632:637	chitin	632:637	However, we recently developed a microwave-intensified biorefinery that efficiently produced chitin, proteins/peptides, and minerals from lobster shells.
37367683	4	48	from	shells	685:690	arg1	proteins/peptides					640:656	proteins/peptides	640:656	proteins/peptides	640:656	However, we recently developed a microwave-intensified biorefinery that efficiently produced chitin, proteins/peptides, and minerals from lobster shells.
37367683	4	48	from	shells	685:690	arg1	minerals					663:670	minerals	663:670	minerals	663:670	However, we recently developed a microwave-intensified biorefinery that efficiently produced chitin, proteins/peptides, and minerals from lobster shells.
37367683	8	49	dep	supplement	1352:1361	arg1	mg/g					1381:1384	139 vs. 148 mg/g	1369:1384	139 vs. 148 mg/g	1369:1384	The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g).
37367683	8	49	dep	supplement	1352:1361	arg1	CCS					1364:1366	CCS	1364:1366	CCS	1364:1366	The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g).
37367683	9	50	dep	lactate	1516:1522	arg1	CCL					1525:1527	CCL	1525:1527	CCL	1525:1527	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	9	51	dep	casein	1644:1649	arg1	mL/g					1680:1683	2.5 vs. 1.5 and 1.0 mL/g	1660:1683	2.5 vs. 1.5 and 1.0 mL/g	1660:1683	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	15	52	theme	%	2726:2726	arg1	supplementation					2705:2719	a lobster mineral supplementation	2687:2719	a lobster mineral supplementation of 15%	2687:2726	Furthermore, the MG-63 and HaCaT cells proliferated considerably after seven days of incubation, reaching 100.3% for MG-63 and 115.9% for HaCaT with a lobster mineral supplementation of 15%.
37367683	11	53	theme	%	2046:2046	arg1	ratios					2029:2034	ratios	2029:2034	ratios of 15%, 25%, and 35% (v/v) when growing cells	2029:2080	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	3	54	theme	value-added	517:527	arg1	products					529:536	value-added products	517:536	value-added products	517:536	Shell chitin conventionally produced through harsh chemical processes is environmentally unfriendly and infeasible for recovering compatible proteins and minerals for value-added products.
37367683	0	55	theme	Shell-Derived	47:59	arg1	Calcium					61:67	Lobster Shell-Derived Calcium	39:67	Lobster Shell-Derived Calcium	39:67	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	9	56	theme	lobster	1424:1430	arg1	%					1443:1443	2%	1442:1443	2%	1442:1443	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	9	56	theme	lobster	1424:1430	arg1	minerals					1432:1439	lobster minerals	1424:1439	lobster minerals (2%, w/w)	1424:1449	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	6	57	theme	commercial	958:967	arg1	applications					969:980	commercial applications	958:980	commercial applications	958:980	This has suggested a further investigation of lobster minerals for commercial applications.
37367683	9	58	theme	higher	1599:1604	arg1	capacity					1618:1625	a considerably higher oil binding capacity	1584:1625	a considerably higher oil binding capacity	1584:1625	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	13	59	theme	bone	2387:2390	arg1	MG-63					2399:2403	MG-63	2399:2403	MG-63	2399:2403	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	13	59	theme	bone	2387:2390	arg1	cells					2392:2396	the bone cells	2383:2396	the bone cells (MG-63)	2383:2404	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	16	60	theme	detectable	2838:2847	arg1	changes					2849:2855	no detectable changes	2835:2855	no detectable changes	2835:2855	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	6	61	theme	minerals	945:952	arg1	investigation					920:932	a further investigation	910:932	a further investigation of lobster minerals for commercial applications	910:980	This has suggested a further investigation of lobster minerals for commercial applications.
37367683	9	62	theme	binding	1610:1616	arg1	capacity					1618:1625	a considerably higher oil binding capacity	1584:1625	a considerably higher oil binding capacity	1584:1625	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	14	63	theme	cell	2463:2466	arg1	growth					2468:2473	The cell growth	2459:2473	The cell growth	2459:2473	The cell growth reached 49.9-61.6% for the MG-63 and 42.9-53.4% for the HaCaT.
37367683	11	64	theme	cell	2123:2126	arg1	morphology					2128:2137	cell morphology	2123:2137	cell morphology	2123:2137	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	16	65	theme	THP-1	2742:2746	arg1	Macrophages					2729:2739	Macrophages	2729:2739	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL	2729:2829	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	16	65	theme	THP-1	2742:2746	arg1	cells					2748:2752	THP-1 cells	2742:2752	THP-1 cells	2742:2752	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	5	66	theme	commercial	870:879	arg1	products					881:888	many commercial products	865:888	many commercial products	865:888	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	5	67	theme	originated	759:768	arg1	calcium					770:776	biologically originated calcium	746:776	biologically originated calcium	746:776	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	7	68	theme	nutritional	1002:1012	arg1	attributes					1014:1023	the nutritional attributes	998:1023	the nutritional attributes	998:1023	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	11	69	theme	lobster	2000:2006	arg1	minerals					2008:2015	lobster minerals	2000:2015	lobster minerals	2000:2015	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	16	70	theme	lobster	2777:2783	arg1	minerals					2785:2792	lobster minerals	2777:2792	lobster minerals at concentrations of 1.24-2.89 mg/mL	2777:2829	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	5	71	theme	calcium-rich	717:728	arg1	composition					730:740	a calcium-rich composition	715:740	a calcium-rich composition	715:740	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	2	72	theme	adverse	301:307	arg1	impacts					309:315	their adverse impacts	295:315	their adverse impacts	295:315	Utilizing these undervalued shells for commercial chitin production could minimize their adverse impacts while maximizing economic value.
37367683	7	73	theme	THP-1	1241:1245	arg1	cells					1248:1252	macrophage (THP-1) cells	1229:1252	macrophage (THP-1) cells	1229:1252	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	5	74	contain	have	710:713	arg1	minerals					701:708	Lobster minerals	693:708	Lobster minerals	693:708	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	5	74	contain	have	710:713	arg2	composition					730:740	a calcium-rich composition	715:740	a calcium-rich composition	715:740	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	17	75	used	used	3017:3020	arg2	minerals					2999:3006	lobster minerals	2991:3006	lobster minerals	2991:3006	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	17	75	used	used	3017:3020	arg2	source					3027:3032	a source	3025:3032	a source of functional or nutraceutical calcium for commercial products	3025:3095	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	2	76	theme	economic	334:341	arg1	value					343:347	economic value	334:347	economic value	334:347	Utilizing these undervalued shells for commercial chitin production could minimize their adverse impacts while maximizing economic value.
37367683	9	77	dep	%	1443:1443	arg1	w/w					1446:1448	w/w	1446:1448	w/w	1446:1448	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	8	78	theme	commercial	1333:1342	arg1	supplement					1352:1361	a commercial calcium supplement	1331:1361	a commercial calcium supplement (CCS, 139 vs. 148 mg/g)	1331:1385	The calcium from the lobster minerals was found to be comparable to that of a commercial calcium supplement (CCS, 139 vs. 148 mg/g).
37367683	5	79	from	ingredient	851:860	arg1	products					881:888	many commercial products	865:888	many commercial products	865:888	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	2	80	theme	commercial	251:260	arg1	production					269:278	commercial chitin production	251:278	commercial chitin production	251:278	Utilizing these undervalued shells for commercial chitin production could minimize their adverse impacts while maximizing economic value.
37367683	9	81	theme	commercial	1497:1506	arg1	lactate					1516:1522	commercial calcium lactate	1497:1522	commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%)	1497:1553	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	15	82	theme	mineral	2697:2703	arg1	supplementation					2705:2719	a lobster mineral supplementation	2687:2719	a lobster mineral supplementation of 15%	2687:2726	Furthermore, the MG-63 and HaCaT cells proliferated considerably after seven days of incubation, reaching 100.3% for MG-63 and 115.9% for HaCaT with a lobster mineral supplementation of 15%.
37367683	10	83	theme	calcium	1883:1889	arg1	5.9-fold					1895:1902	5.9-fold	1895:1902	5.9-fold	1895:1902	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	83	theme	calcium	1883:1889	arg1	bioavailability					1856:1870	the in vitro bioavailability	1843:1870	the in vitro bioavailability of lobster calcium	1843:1889	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	83	theme	calcium	1883:1889	arg1	higher					1904:1909	higher	1904:1909	higher	1904:1909	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	7	84	theme	gastrointestinal	1148:1163	arg1	digestion					1165:1173	in vitro simulated gastrointestinal digestion	1129:1173	in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells	1129:1252	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	15	85	theme	HaCaT	2565:2569	arg1	cells					2571:2575	the MG-63 and HaCaT cells	2551:2575	cells	2571:2575	Furthermore, the MG-63 and HaCaT cells proliferated considerably after seven days of incubation, reaching 100.3% for MG-63 and 115.9% for HaCaT with a lobster mineral supplementation of 15%.
37367683	1	86	theme	Shell	131:135	arg1	wastes					137:142	Shell wastes	131:142	Shell wastes	131:142	Shell wastes pose environmental and financial burdens to the shellfish industry.
37367683	12	87	dep	cell	2193:2196	arg1	growth					2198:2203	growth	2198:2203	growth	2198:2203	However, it had significant effects on cell growth and proliferation.
37367683	12	87	dep	cell	2193:2196	arg1	proliferation					2209:2221	proliferation	2209:2221	proliferation	2209:2221	However, it had significant effects on cell growth and proliferation.
37367683	4	88	theme	microwave-intensified	572:592	arg1	biorefinery					594:604	a microwave-intensified biorefinery	570:604	a microwave-intensified biorefinery that efficiently produced chitin, proteins/peptides, and minerals from lobster shells	570:690	However, we recently developed a microwave-intensified biorefinery that efficiently produced chitin, proteins/peptides, and minerals from lobster shells.
37367683	0	89	theme	Bone	101:104	arg1	Cells					115:119	Significantly Proliferating Bone and Skin Cells	73:119	Significantly Proliferating Bone and Skin Cells	73:119	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	13	90	theme	lobster	2297:2303	arg1	minerals					2305:2312	the lobster minerals	2293:2312	the lobster minerals	2293:2312	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	0	91	theme	Skin	110:113	arg1	Cells					115:119	Significantly Proliferating Bone and Skin Cells	73:119	Significantly Proliferating Bone and Skin Cells	73:119	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	7	92	theme	lobster	1092:1098	arg1	minerals					1100:1107	lobster minerals	1092:1107	lobster minerals	1092:1107	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	13	93	theme	cells	2241:2245	arg1	responses					2228:2236	The responses	2224:2236	The responses	2224:2236	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	13	93	theme	cells	2241:2245	arg1	better					2371:2376	better	2371:2376	better	2371:2376	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	11	94	theme	growing	2068:2074	arg1	cells					2076:2080	growing cells	2068:2080	growing cells	2068:2080	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	10	95	dep	in	1847:1848	arg1	vitro					1850:1854	vitro	1850:1854	vitro	1850:1854	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	12	96	contain	had	2166:2168	arg2	effects					2182:2188	significant effects	2170:2188	significant effects	2170:2188	However, it had significant effects on cell growth and proliferation.
37367683	12	96	contain	had	2166:2168	arg1	it					2163:2164	it	2163:2164	it	2163:2164	However, it had significant effects on cell growth and proliferation.
37367683	7	97	dep	in	1129:1130	arg1	vitro					1132:1136	vitro	1132:1136	vitro	1132:1136	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	5	98	theme	nutraceutical	837:849	arg1	ingredient					851:860	a functional, dietary, or nutraceutical ingredient	811:860	a functional, dietary, or nutraceutical ingredient in many commercial products	811:888	Lobster minerals have a calcium-rich composition and biologically originated calcium is more biofunctional for use as a functional, dietary, or nutraceutical ingredient in many commercial products.
37367683	3	99	theme	harsh	395:399	arg1	processes					410:418	harsh chemical processes	395:418	harsh chemical processes	395:418	Shell chitin conventionally produced through harsh chemical processes is environmentally unfriendly and infeasible for recovering compatible proteins and minerals for value-added products.
37367683	13	100	theme	quick	2424:2428	arg1	HaCaT					2451:2455	HaCaT	2451:2455	HaCaT	2451:2455	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	13	100	theme	quick	2424:2428	arg1	cells					2444:2448	competitively quick with the skin cells	2410:2448	competitively quick with the skin cells (HaCaT)	2410:2456	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	11	101	theme	%	2041:2041	arg1	ratios					2029:2034	ratios	2029:2034	ratios of 15%, 25%, and 35% (v/v) when growing cells	2029:2080	Furthermore, supplementing lobster minerals in media at ratios of 15%, 25%, and 35% (v/v) when growing cells did not induce any detectable changes in cell morphology and apoptosis.
37367683	17	102	theme	lobster	2991:2997	arg1	minerals					2999:3006	lobster minerals	2991:3006	lobster minerals	2991:3006	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	17	102	theme	lobster	2991:2997	arg1	source					3027:3032	a source	3025:3032	a source of functional or nutraceutical calcium for commercial products	3025:3095	All these results indicate that lobster minerals could be used as a source of functional or nutraceutical calcium for commercial products.
37367683	9	103	dep	its	1632:1634	arg1	rivals					1636:1641	rivals	1636:1641	rivals	1636:1641	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	7	104	theme	nutraceutical	1049:1061	arg1	effects					1063:1069	nutraceutical effects	1049:1069	nutraceutical effects	1049:1069	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	13	105	with	quick	2424:2428	arg1	skin					2439:2442	the skin	2435:2442	the skin	2435:2442	The responses of cells after three days of culture supplemented with the lobster minerals, compared to the CCS supplementation, were significantly better with the bone cells (MG-63) and competitively quick with the skin cells (HaCaT).
37367683	0	106	theme	Lobster	39:45	arg1	Calcium					61:67	Lobster Shell-Derived Calcium	39:67	Lobster Shell-Derived Calcium	39:67	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	3	107	theme	compatible	480:489	arg1	proteins					491:498	compatible proteins	480:498	compatible proteins	480:498	Shell chitin conventionally produced through harsh chemical processes is environmentally unfriendly and infeasible for recovering compatible proteins and minerals for value-added products.
37367683	0	108	theme	Calcium	61:67	arg1	Bioavailability					20:34	Bioavailability	20:34	Bioavailability	20:34	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	0	108	theme	Calcium	61:67	arg1	Solubility					5:14	High Solubility	0:14	High Solubility	0:14	High Solubility and Bioavailability of Lobster Shell-Derived Calcium for Significantly Proliferating Bone and Skin Cells In Vitro.
37367683	7	109	theme	functional	1026:1035	arg1	properties					1037:1046	functional properties	1026:1046	functional properties	1026:1046	In this study, the nutritional attributes, functional properties, nutraceutical effects, and cytotoxicity of lobster minerals were analyzed using in vitro simulated gastrointestinal digestion combined with growing bone (MG-63), skin (HaCaT), and macrophage (THP-1) cells.
37367683	9	110	theme	oil	1606:1608	arg1	capacity					1618:1625	a considerably higher oil binding capacity	1584:1625	a considerably higher oil binding capacity	1584:1625	In addition, beef incorporated with lobster minerals (2%, w/w) retained water better than that of casein and commercial calcium lactate (CCL, 21.1 vs. 15.1 and 13.3%), and the lobster mineral had a considerably higher oil binding capacity than its rivals (casein and CCL, 2.5 vs. 1.5 and 1.0 mL/g).
37367683	16	111	from	concentrations	2797:2810	arg1	minerals					2785:2792	lobster minerals	2777:2792	lobster minerals at concentrations of 1.24-2.89 mg/mL	2777:2829	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	10	112	theme	11.95	1955:1959	arg1	%					1969:1969	11.95 vs. 1.99%	1955:1969	%	1969:1969	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	10	112	theme	11.95	1955:1959	arg1	product					1946:1952	the commercial product	1931:1952	the commercial product (11.95 vs. 1.99%)	1931:1970	Notably, the lobster mineral and its calcium were far more soluble than the CCS (98.4 vs. 18.6% for the products and 64.0 vs. 8.5% for their calcium) while the in vitro bioavailability of lobster calcium was 5.9-fold higher compared to that of the commercial product (11.95 vs. 1.99%).
37367683	16	113	theme	cell	2860:2863	arg1	morphology					2865:2874	cell morphology	2860:2874	cell morphology	2860:2874	Macrophages (THP-1 cells) treated for 24 h with lobster minerals at concentrations of 1.24-2.89 mg/mL had no detectable changes in cell morphology while their viability was over 82.2%, far above the cytotoxicity threshold (<70%).
37367683	6	114	theme	lobster	937:943	arg1	minerals					945:952	lobster minerals	937:952	lobster minerals	937:952	This has suggested a further investigation of lobster minerals for commercial applications.
37060979	3	0	theme	rice	842:845	arg1	hardness					853:860	frozen rice dough hardness	835:860	frozen rice dough hardness	835:860	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	1	theme	process	588:594	arg1	damage					569:574	the damage	565:574	the damage of freezing process to starch granules and protein structure in frozen rice dough	565:656	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	1	2	theme	rice	248:251	arg1	starch					253:258	glutinous rice starch	238:258	glutinous rice starch	238:258	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	3	theme	elasticity	866:875	arg1	decrease					882:889	the decrease	878:889	the decrease of storage modulus (G')	878:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	3	theme	elasticity	866:875	arg1	decrease					718:725	the decrease	714:725	the decrease of protein ordered structure	714:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	3	theme	elasticity	866:875	arg1	decrease					823:830	the decrease	819:830	the decrease of frozen rice dough hardness and elasticity	819:875	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	3	theme	elasticity	866:875	arg1	change					761:766	the change	757:766	the change of bound water in frozen rice dough to free water	757:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	3	theme	elasticity	866:875	arg1	increase					686:693	the increase	682:693	the increase of damaged starch	682:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	3	theme	elasticity	866:875	arg1	deterioration					923:935	the deterioration	919:935	the deterioration of frozen rice dough texture	919:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	4	theme	10 	1233:1235	arg1	gluten					1238:1243	adding 10 % gluten	1226:1243	adding 10 % gluten	1226:1243	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	1	5	theme	different	165:173	arg1	methods					184:190	four different freezing methods	160:190	four different freezing methods	160:190	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	6	theme	modulus	902:908	arg1	decrease					882:889	the decrease	878:889	the decrease of storage modulus (G')	878:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	6	theme	modulus	902:908	arg1	decrease					718:725	the decrease	714:725	the decrease of protein ordered structure	714:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	6	theme	modulus	902:908	arg1	decrease					823:830	the decrease	819:830	the decrease of frozen rice dough hardness and elasticity	819:875	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	6	theme	modulus	902:908	arg1	change					761:766	the change	757:766	the change of bound water in frozen rice dough to free water	757:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	6	theme	modulus	902:908	arg1	increase					686:693	the increase	682:693	the increase of damaged starch	682:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	6	theme	modulus	902:908	arg1	deterioration					923:935	the deterioration	919:935	the deterioration of frozen rice dough texture	919:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	4	7	theme	dough	1184:1188	arg1	characteristics					1153:1167	the viscosity characteristics	1139:1167	the viscosity characteristics of frozen rice dough	1139:1188	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	1	8	theme	methods	184:190	arg1	effects					149:155	the effects	145:155	the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten	145:269	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	1	8	theme	methods	184:190	arg1	changes					280:286	the changes	276:286	the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment	276:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	9	theme	frozen	640:645	arg1	dough					652:656	frozen rice dough	640:656	frozen rice dough	640:656	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	1	10	theme	nitrogen	358:365	arg1	treatment					372:380	liquid nitrogen (LF) treatment	351:380	liquid nitrogen (LF) treatment	351:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	11	theme	structure	746:754	arg1	decrease					882:889	the decrease	878:889	the decrease of storage modulus (G')	878:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	11	theme	structure	746:754	arg1	decrease					718:725	the decrease	714:725	the decrease of protein ordered structure	714:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	11	theme	structure	746:754	arg1	decrease					823:830	the decrease	819:830	the decrease of frozen rice dough hardness and elasticity	819:875	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	11	theme	structure	746:754	arg1	change					761:766	the change	757:766	the change of bound water in frozen rice dough to free water	757:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	11	theme	structure	746:754	arg1	increase					686:693	the increase	682:693	the increase of damaged starch	682:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	11	theme	structure	746:754	arg1	deterioration					923:935	the deterioration	919:935	the deterioration of frozen rice dough texture	919:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	12	theme	damaged	698:704	arg1	starch					706:711	damaged starch	698:711	damaged starch	698:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	13	theme	protein	730:736	arg1	structure					746:754	protein ordered structure	730:754	protein ordered structure	730:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	4	14	theme	gluten	983:988	arg1	addition					971:978	The addition	967:978	The addition of gluten in frozen rice dough	967:1009	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	0	15	theme	rice	59:62	arg1	dough					64:68	frozen rice dough	52:68	frozen rice dough	52:68	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	1	16	theme	rice	305:308	arg1	dough					310:314	rice dough	305:314	rice dough with different gluten ratios after liquid nitrogen (LF) treatment	305:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	4	17	theme	frozen	993:998	arg1	dough					1005:1009	frozen rice dough	993:1009	frozen rice dough	993:1009	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	1	18	from	effects	149:155	arg1	texture					199:205	the texture	195:205	the texture of rice dough reconstituted by glutinous rice starch and gluten	195:269	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	19	theme	rate	559:562	arg1	down					542:545	the slowing down	530:545	the slowing down of freezing rate	530:562	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	4	20	theme	crystal	1063:1069	arg1	structure					1071:1079	crystal structure	1063:1079	crystal structure	1063:1079	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	0	21	theme	glutinous	87:95	arg1	starch					102:107	glutinous rice starch	87:107	glutinous rice starch	87:107	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	1	22	theme	gluten	331:336	arg1	ratios					338:343	different gluten ratios	321:343	different gluten ratios after liquid nitrogen (LF) treatment	321:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	23	theme	frozen	786:791	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	24	theme	frozen	1273:1278	arg1	dough					1285:1289	frozen rice dough	1273:1289	frozen rice dough	1273:1289	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	3	25	theme	starch	706:711	arg1	decrease					882:889	the decrease	878:889	the decrease of storage modulus (G')	878:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	25	theme	starch	706:711	arg1	decrease					718:725	the decrease	714:725	the decrease of protein ordered structure	714:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	25	theme	starch	706:711	arg1	decrease					823:830	the decrease	819:830	the decrease of frozen rice dough hardness and elasticity	819:875	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	25	theme	starch	706:711	arg1	change					761:766	the change	757:766	the change of bound water in frozen rice dough to free water	757:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	25	theme	starch	706:711	arg1	increase					686:693	the increase	682:693	the increase of damaged starch	682:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	25	theme	starch	706:711	arg1	deterioration					923:935	the deterioration	919:935	the deterioration of frozen rice dough texture	919:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	26	theme	water	777:781	arg1	decrease					882:889	the decrease	878:889	the decrease of storage modulus (G')	878:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	26	theme	water	777:781	arg1	decrease					718:725	the decrease	714:725	the decrease of protein ordered structure	714:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	26	theme	water	777:781	arg1	decrease					823:830	the decrease	819:830	the decrease of frozen rice dough hardness and elasticity	819:875	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	26	theme	water	777:781	arg1	change					761:766	the change	757:766	the change of bound water in frozen rice dough to free water	757:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	26	theme	water	777:781	arg1	increase					686:693	the increase	682:693	the increase of damaged starch	682:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	26	theme	water	777:781	arg1	deterioration					923:935	the deterioration	919:935	the deterioration of frozen rice dough texture	919:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	2	27	theme	dough	411:415	arg1	profiles					387:394	The profiles	383:394	The profiles of frozen rice dough	383:415	The profiles of frozen rice dough were studied by texture analyzer, low-field NMR, SEM, FT-IR, DSC, CLSM, X-RD and RVA.
37060979	4	28	theme	short-range	1029:1039	arg1	structure					1049:1057	short-range ordered structure	1029:1057	short-range ordered structure	1029:1057	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	3	29	theme	free	807:810	arg1	water					812:816	free water	807:816	free water	807:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	2	30	theme	frozen	399:404	arg1	dough					411:415	frozen rice dough	399:415	frozen rice dough	399:415	The profiles of frozen rice dough were studied by texture analyzer, low-field NMR, SEM, FT-IR, DSC, CLSM, X-RD and RVA.
37060979	5	31	theme	rice	1332:1335	arg1	texture					1343:1349	frozen rice dough texture	1325:1349	frozen rice dough texture	1325:1349	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	0	32	theme	freezing	16:23	arg1	methods					25:31	freezing methods	16:31	freezing methods	16:31	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	3	33	theme	texture	958:964	arg1	decrease					882:889	the decrease	878:889	the decrease of storage modulus (G')	878:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	33	theme	texture	958:964	arg1	decrease					718:725	the decrease	714:725	the decrease of protein ordered structure	714:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	33	theme	texture	958:964	arg1	decrease					823:830	the decrease	819:830	the decrease of frozen rice dough hardness and elasticity	819:875	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	33	theme	texture	958:964	arg1	change					761:766	the change	757:766	the change of bound water in frozen rice dough to free water	757:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	33	theme	texture	958:964	arg1	increase					686:693	the increase	682:693	the increase of damaged starch	682:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	33	theme	texture	958:964	arg1	deterioration					923:935	the deterioration	919:935	the deterioration of frozen rice dough texture	919:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	2	34	theme	texture	433:439	arg1	analyzer					441:448	texture analyzer	433:448	texture analyzer	433:448	The profiles of frozen rice dough were studied by texture analyzer, low-field NMR, SEM, FT-IR, DSC, CLSM, X-RD and RVA.
37060979	3	35	from	decrease	718:725	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	36	theme	rice	947:950	arg1	texture					958:964	frozen rice dough texture	940:964	frozen rice dough texture	940:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	37	from	increase	686:693	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	38	theme	freezing	550:557	arg1	rate					559:562	freezing rate	550:562	freezing rate	550:562	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	1	39	theme	properties	291:300	arg1	effects					149:155	the effects	145:155	the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten	145:269	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	1	39	theme	properties	291:300	arg1	changes					280:286	the changes	276:286	the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment	276:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	4	40	from	dough	1005:1009	arg1	addition					971:978	The addition	967:978	The addition of gluten in frozen rice dough	967:1009	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	3	41	theme	freezing	579:586	arg1	process					588:594	freezing process	579:594	freezing process	579:594	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	42	theme	adding	1226:1231	arg1	gluten					1238:1243	adding 10 % gluten	1226:1243	adding 10 % gluten	1226:1243	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	4	43	dep	structure	1049:1057	arg1	the					1025:1027	the	1025:1027	the	1025:1027	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	1	44	theme	rice	210:213	arg1	dough					215:219	rice dough	210:219	rice dough reconstituted by glutinous rice starch and gluten	210:269	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	45	theme	dough	847:851	arg1	hardness					853:860	frozen rice dough hardness	835:860	frozen rice dough hardness	835:860	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	4	46	from	addition	971:978	arg1	dough					1005:1009	frozen rice dough	993:1009	frozen rice dough	993:1009	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	4	47	from	gluten	983:988	arg1	dough					1005:1009	frozen rice dough	993:1009	frozen rice dough	993:1009	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	3	48	theme	frozen	835:840	arg1	hardness					853:860	frozen rice dough hardness	835:860	frozen rice dough hardness	835:860	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	4	49	theme	viscosity	1143:1151	arg1	characteristics					1153:1167	the viscosity characteristics	1139:1167	the viscosity characteristics of frozen rice dough	1139:1188	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	5	50	theme	least	1309:1313	arg1	effect					1315:1320	the least effect	1305:1320	the least effect	1305:1320	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	1	51	theme	glutinous	238:246	arg1	starch					253:258	glutinous rice starch	238:258	glutinous rice starch	238:258	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	4	52	theme	rice	1179:1182	arg1	dough					1184:1188	frozen rice dough	1172:1188	frozen rice dough	1172:1188	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	1	53	from	changes	280:286	arg1	texture					199:205	the texture	195:205	the texture of rice dough reconstituted by glutinous rice starch and gluten	195:269	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	54	from	change	761:766	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	55	theme	storage	894:900	arg1	G					911:911	G'	911:912	G'	911:912	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	55	theme	storage	894:900	arg1	modulus					902:908	storage modulus	894:908	storage modulus (G')	894:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	56	theme	protein	619:625	arg1	structure					627:635	protein structure	619:635	protein structure	619:635	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	57	theme	%	1236:1236	arg1	gluten					1238:1243	adding 10 % gluten	1226:1243	adding 10 % gluten	1226:1243	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	1	58	theme	freezing	175:182	arg1	methods					184:190	four different freezing methods	160:190	four different freezing methods	160:190	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	59	from	deterioration	923:935	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	60	theme	rice	647:650	arg1	dough					652:656	frozen rice dough	640:656	frozen rice dough	640:656	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	61	theme	slowing	534:540	arg1	down					542:545	the slowing down	530:545	the slowing down of freezing rate	530:562	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	62	contain	has	1301:1303	arg1	LF					1298:1299	LF	1298:1299	LF	1298:1299	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	5	62	contain	has	1301:1303	arg2	effect					1315:1320	the least effect	1305:1320	the least effect	1305:1320	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	0	63	theme	dough	64:68	arg1	composition					37:47	composition	37:47	composition	37:47	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	0	63	theme	dough	64:68	arg1	Optimization					0:11	Optimization	0:11	Optimization of freezing methods	0:31	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	3	64	theme	ordered	738:744	arg1	structure					746:754	protein ordered structure	730:754	protein ordered structure	730:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	2	65	theme	low-field	451:459	arg1	NMR					461:463	low-field NMR	451:463	low-field NMR	451:463	The profiles of frozen rice dough were studied by texture analyzer, low-field NMR, SEM, FT-IR, DSC, CLSM, X-RD and RVA.
37060979	4	66	theme	rice	1000:1003	arg1	dough					1005:1009	frozen rice dough	993:1009	frozen rice dough	993:1009	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	1	67	theme	dough	310:314	arg1	properties					291:300	properties	291:300	properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment	291:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	68	theme	bound	771:775	arg1	water					777:781	bound water	771:781	bound water	771:781	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	0	69	theme	rice	97:100	arg1	starch					102:107	glutinous rice starch	87:107	glutinous rice starch	87:107	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	1	70	theme	different	321:329	arg1	ratios					338:343	different gluten ratios	321:343	different gluten ratios after liquid nitrogen (LF) treatment	321:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	1	71	with	dough	310:314	arg1	ratios					338:343	different gluten ratios	321:343	different gluten ratios after liquid nitrogen (LF) treatment	321:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	4	72	theme	starch	1084:1089	arg1	structure					1049:1057	short-range ordered structure	1029:1057	short-range ordered structure	1029:1057	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	4	72	theme	starch	1084:1089	arg1	structure					1071:1079	crystal structure	1063:1079	crystal structure	1063:1079	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	3	73	theme	rice	793:796	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	74	theme	rice	1280:1283	arg1	dough					1285:1289	frozen rice dough	1273:1289	frozen rice dough	1273:1289	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	4	75	theme	ordered	1041:1047	arg1	structure					1049:1057	short-range ordered structure	1029:1057	short-range ordered structure	1029:1057	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	1	76	theme	liquid	351:356	arg1	LF					368:369	LF	368:369	LF	368:369	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	1	76	theme	liquid	351:356	arg1	nitrogen					358:365	liquid nitrogen	351:365	liquid nitrogen (LF) treatment	351:380	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	2	77	theme	rice	406:409	arg1	dough					411:415	frozen rice dough	399:415	frozen rice dough	399:415	The profiles of frozen rice dough were studied by texture analyzer, low-field NMR, SEM, FT-IR, DSC, CLSM, X-RD and RVA.
37060979	0	78	theme	methods	25:31	arg1	composition					37:47	composition	37:47	composition	37:47	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	0	78	theme	methods	25:31	arg1	Optimization					0:11	Optimization	0:11	Optimization of freezing methods	0:31	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	3	79	theme	dough	952:956	arg1	texture					958:964	frozen rice dough texture	940:964	frozen rice dough texture	940:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	80	theme	dough	1337:1341	arg1	texture					1343:1349	frozen rice dough texture	1325:1349	frozen rice dough texture	1325:1349	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	4	81	theme	starch	1120:1125	arg1	digestibility					1103:1115	the digestibility	1099:1115	the digestibility of starch	1099:1125	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	3	82	theme	frozen	940:945	arg1	texture					958:964	frozen rice dough texture	940:964	frozen rice dough texture	940:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	83	from	decrease	823:830	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	5	84	theme	experimental	1204:1215	arg1	results					1217:1223	the experimental results	1200:1223	the experimental results	1200:1223	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	0	85	theme	frozen	52:57	arg1	dough					64:68	frozen rice dough	52:68	frozen rice dough	52:68	Optimization of freezing methods and composition of frozen rice dough reconstituted by glutinous rice starch and gluten.
37060979	3	86	from	decrease	882:889	arg1	dough					798:802	frozen rice dough	786:802	frozen rice dough to free water	786:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	4	87	theme	frozen	1172:1177	arg1	dough					1184:1188	frozen rice dough	1172:1188	frozen rice dough	1172:1188	The addition of gluten in frozen rice dough will increase the short-range ordered structure and crystal structure of starch, reduce the digestibility of starch, and change the viscosity characteristics of frozen rice dough.
37060979	5	88	theme	frozen	1325:1330	arg1	texture					1343:1349	frozen rice dough texture	1325:1349	frozen rice dough texture	1325:1349	Based on the experimental results, adding 10 % gluten is more suitable for making frozen rice dough, while LF has the least effect on frozen rice dough texture.
37060979	1	89	theme	dough	215:219	arg1	texture					199:205	the texture	195:205	the texture of rice dough reconstituted by glutinous rice starch and gluten	195:269	This study investigated the effects of four different freezing methods on the texture of rice dough reconstituted by glutinous rice starch and gluten, and the changes of properties of rice dough with different gluten ratios after liquid nitrogen (LF) treatment.
37060979	3	90	theme	hardness	853:860	arg1	decrease					882:889	the decrease	878:889	the decrease of storage modulus (G')	878:913	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	90	theme	hardness	853:860	arg1	decrease					718:725	the decrease	714:725	the decrease of protein ordered structure	714:754	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	90	theme	hardness	853:860	arg1	decrease					823:830	the decrease	819:830	the decrease of frozen rice dough hardness and elasticity	819:875	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	90	theme	hardness	853:860	arg1	change					761:766	the change	757:766	the change of bound water in frozen rice dough to free water	757:816	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	90	theme	hardness	853:860	arg1	increase					686:693	the increase	682:693	the increase of damaged starch	682:711	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	90	theme	hardness	853:860	arg1	deterioration					923:935	the deterioration	919:935	the deterioration of frozen rice dough texture	919:964	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	91	from	damage	569:574	arg1	dough					652:656	frozen rice dough	640:656	frozen rice dough	640:656	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37060979	3	92	theme	starch	599:604	arg1	granules					606:613	starch granules	599:613	starch granules	599:613	Results revealed that with the slowing down of freezing rate, the damage of freezing process to starch granules and protein structure in frozen rice dough increases, resulting in the increase of damaged starch, the decrease of protein ordered structure, the change of bound water in frozen rice dough to free water, the decrease of frozen rice dough hardness and elasticity, the decrease of storage modulus (G') and the deterioration of frozen rice dough texture.
37344477	1	0	with	disorder	172:179	arg1	heterogeneity					195:207	cellular heterogeneity	186:207	cellular heterogeneity	186:207	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	6	1	theme	disappearance	1138:1150	arg1	reaction					1152:1159	the observed macrophage disappearance reaction	1114:1159	the observed macrophage disappearance reaction of resident macrophages	1114:1183	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	2	2	theme	single-cell	377:387	arg1	landscape					389:397	the single-cell landscape	373:397	the single-cell landscape of the human colon ecosystem	373:426	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	5	3	theme	B	1056:1056	arg1	cells					1058:1062	T and B cells	1050:1062	T and B cells	1050:1062	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	4	4	theme	resident	743:750	arg1	macrophages					752:762	resident macrophages	743:762	resident macrophages	743:762	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	5	5	theme	ulcerative	985:994	arg1	colitis					996:1002	ulcerative colitis	985:1002	ulcerative colitis	985:1002	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	4	6	theme	resident	858:865	arg1	macrophages					867:877	resident macrophages	858:877	resident macrophages	858:877	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	2	7	theme	imaging	309:315	arg1	cytometry					322:330	imaging mass cytometry	309:330	imaging mass cytometry	309:330	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	2	8	theme	mass	317:320	arg1	cytometry					322:330	imaging mass cytometry	309:330	imaging mass cytometry	309:330	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	4	9	theme	ulcerative	643:652	arg1	colitis					654:660	the ulcerative colitis	639:660	the ulcerative colitis region	639:667	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	0	10	theme	ulcerative	101:110	arg1	colitis					112:118	ulcerative colitis	101:118	ulcerative colitis	101:118	Selective oxidative protection leads to tissue topological changes orchestrated by macrophage during ulcerative colitis.
37344477	4	11	located	detected	731:738	arg2	SOD2					716:719	reactive oxygen species scavenging enzyme SOD2	674:719	reactive oxygen species scavenging enzyme SOD2	674:719	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	4	11	located	detected	731:738	arg1	macrophages					752:762	resident macrophages	743:762	resident macrophages	743:762	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	6	12	theme	mechanistic	1196:1206	arg1	hints					1208:1212	mechanistic hints	1196:1212	mechanistic hints	1196:1212	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	1	13	theme	chronic	145:151	arg1	colitis					132:138	Ulcerative colitis	121:138	Ulcerative colitis	121:138	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	1	13	theme	chronic	145:151	arg1	disorder					172:179	a chronic inflammatory bowel disorder	143:179	a chronic inflammatory bowel disorder with cellular heterogeneity	143:207	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	4	14	theme	oxygen	796:801	arg1	species					803:809	distinct reactive oxygen species	778:809	distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages	778:877	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	3	15	theme	tissue-resident	565:579	arg1	macrophages					581:591	tissue-resident macrophages	565:591	tissue-resident macrophages	565:591	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	3	16	theme	topological	444:454	arg1	changes					456:462	tissue topological changes	437:462	tissue topological changes	437:462	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	1	17	theme	cellular	186:193	arg1	heterogeneity					195:207	cellular heterogeneity	186:207	cellular heterogeneity	186:207	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	5	18	theme	Inflammatory	880:891	arg1	macrophages					893:903	Inflammatory macrophages	880:903	Inflammatory macrophages	880:903	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	6	19	theme	disappearance	1229:1241	arg1	reaction					1243:1250	macrophage disappearance reaction	1218:1250	macrophage disappearance reaction in other inflammation or infection situations	1218:1296	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	2	20	theme	colon	412:416	arg1	ecosystem					418:426	the human colon ecosystem	402:426	the human colon ecosystem	402:426	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	0	21	theme	oxidative	10:18	arg1	protection					20:29	Selective oxidative protection	0:29	Selective oxidative protection	0:29	Selective oxidative protection leads to tissue topological changes orchestrated by macrophage during ulcerative colitis.
37344477	6	22	theme	infection	1277:1285	arg1	situations					1287:1296	other inflammation or infection situations	1255:1296	situations	1287:1296	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	4	23	theme	enzyme	709:714	arg1	SOD2					716:719	reactive oxygen species scavenging enzyme SOD2	674:719	reactive oxygen species scavenging enzyme SOD2	674:719	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	2	24	theme	single-cell	336:346	arg1	sequencing					352:361	single-cell RNA sequencing	336:361	single-cell RNA sequencing	336:361	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	6	25	theme	resident	1164:1171	arg1	macrophages					1173:1183	resident macrophages	1164:1183	resident macrophages	1164:1183	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	2	26	theme	human	406:410	arg1	ecosystem					418:426	the human colon ecosystem	402:426	the human colon ecosystem	402:426	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	0	27	theme	Selective	0:8	arg1	protection					20:29	Selective oxidative protection	0:29	Selective oxidative protection	0:29	Selective oxidative protection leads to tissue topological changes orchestrated by macrophage during ulcerative colitis.
37344477	4	28	theme	species	610:616	arg1	levels					618:623	Reactive oxygen species levels	594:623	Reactive oxygen species levels	594:623	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	6	29	theme	macrophage	1127:1136	arg1	reaction					1152:1159	the observed macrophage disappearance reaction	1114:1159	the observed macrophage disappearance reaction of resident macrophages	1114:1183	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	4	30	theme	reactive	787:794	arg1	species					803:809	distinct reactive oxygen species	778:809	distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages	778:877	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	4	31	theme	inflammatory	829:840	arg1	macrophages					842:852	inflammatory macrophages	829:852	inflammatory macrophages	829:852	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	5	32	theme	production	967:976	arg1	shift					954:958	a spatial shift	944:958	a spatial shift of TNF production during ulcerative colitis	944:1002	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	5	33	theme	resident	913:920	arg1	macrophages					922:932	resident macrophages	913:932	resident macrophages	913:932	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	4	34	theme	oxygen	603:608	arg1	species					610:616	Reactive oxygen species	594:616	Reactive oxygen species levels	594:623	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	5	35	theme	T	1050:1050	arg1	cells					1058:1062	T and B cells	1050:1062	T and B cells	1050:1062	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	6	36	theme	macrophages	1173:1183	arg1	reaction					1152:1159	the observed macrophage disappearance reaction	1114:1159	the observed macrophage disappearance reaction of resident macrophages	1114:1183	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	4	37	theme	oxygen	683:688	arg1	species					690:696	reactive oxygen species	674:696	reactive oxygen species scavenging enzyme SOD2	674:719	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	6	38	theme	macrophage	1218:1227	arg1	reaction					1243:1250	macrophage disappearance reaction	1218:1250	macrophage disappearance reaction in other inflammation or infection situations	1218:1296	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	1	39	theme	Ulcerative	121:130	arg1	colitis					132:138	Ulcerative colitis	121:138	Ulcerative colitis	121:138	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	1	39	theme	Ulcerative	121:130	arg1	disorder					172:179	a chronic inflammatory bowel disorder	143:179	a chronic inflammatory bowel disorder with cellular heterogeneity	143:207	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	4	40	theme	reactive	674:681	arg1	species					690:696	reactive oxygen species	674:696	reactive oxygen species scavenging enzyme SOD2	674:719	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	0	41	theme	tissue	40:45	arg1	changes					59:65	tissue topological changes	40:65	tissue topological changes orchestrated by macrophage during ulcerative colitis	40:118	Selective oxidative protection leads to tissue topological changes orchestrated by macrophage during ulcerative colitis.
37344477	5	42	theme	cytokine	1010:1017	arg1	network					1030:1036	a cytokine production network	1008:1036	a cytokine production network formed with T and B cells	1008:1062	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	4	43	theme	scavenging	698:707	arg1	SOD2					716:719	reactive oxygen species scavenging enzyme SOD2	674:719	reactive oxygen species scavenging enzyme SOD2	674:719	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	3	44	theme	macrophage	478:487	arg1	reaction					503:510	macrophage disappearance reaction	478:510	macrophage disappearance reaction in the ulcerative colitis region	478:543	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	2	45	theme	ecosystem	418:426	arg1	landscape					389:397	the single-cell landscape	373:397	the single-cell landscape of the human colon ecosystem	373:426	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	3	46	theme	tissue	437:442	arg1	changes					456:462	tissue topological changes	437:462	tissue topological changes	437:462	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	5	47	theme	spatial	946:952	arg1	shift					954:958	a spatial shift	944:958	a spatial shift of TNF production during ulcerative colitis	944:1002	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	3	48	theme	disappearance	489:501	arg1	reaction					503:510	macrophage disappearance reaction	478:510	macrophage disappearance reaction in the ulcerative colitis region	478:543	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	5	49	theme	TNF	963:965	arg1	production					967:976	TNF production	963:976	TNF production	963:976	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	6	50	theme	observed	1118:1125	arg1	reaction					1152:1159	the observed macrophage disappearance reaction	1114:1159	the observed macrophage disappearance reaction of resident macrophages	1114:1183	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	3	51	theme	colitis	530:536	arg1	region					538:543	the ulcerative colitis region	515:543	the ulcerative colitis region	515:543	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	0	52	theme	topological	47:57	arg1	changes					59:65	tissue topological changes	40:65	tissue topological changes orchestrated by macrophage during ulcerative colitis	40:118	Selective oxidative protection leads to tissue topological changes orchestrated by macrophage during ulcerative colitis.
37344477	4	53	theme	Reactive	594:601	arg1	species					610:616	Reactive oxygen species	594:616	Reactive oxygen species levels	594:623	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	2	54	theme	spatial	244:250	arg1	changes					252:258	spatial changes	244:258	spatial changes	244:258	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	3	55	from	reaction	503:510	arg1	region					538:543	the ulcerative colitis region	515:543	the ulcerative colitis region	515:543	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	4	56	dep	species	803:809	arg1	vulnerability					811:823	vulnerability	811:823	vulnerability	811:823	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	6	57	from	reaction	1243:1250	arg1	inflammation					1261:1272	other inflammation or infection situations	1255:1296	inflammation	1261:1272	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	6	57	from	reaction	1243:1250	arg1	situations					1287:1296	other inflammation or infection situations	1255:1296	situations	1287:1296	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	4	58	theme	distinct	778:785	arg1	species					803:809	distinct reactive oxygen species	778:809	distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages	778:877	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	5	59	theme	production	1019:1028	arg1	network					1030:1036	a cytokine production network	1008:1036	a cytokine production network formed with T and B cells	1008:1062	Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells.
37344477	2	60	theme	ecosystem	286:294	arg1	composition					228:238	composition	228:238	composition	228:238	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	2	60	theme	ecosystem	286:294	arg1	changes					252:258	spatial changes	244:258	spatial changes	244:258	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	2	61	theme	RNA	348:350	arg1	sequencing					352:361	single-cell RNA sequencing	336:361	single-cell RNA sequencing	336:361	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	4	62	theme	colitis	654:660	arg1	region					662:667	the ulcerative colitis region	639:667	the ulcerative colitis region	639:667	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	4	63	theme	species	690:696	arg1	SOD2					716:719	reactive oxygen species scavenging enzyme SOD2	674:719	reactive oxygen species scavenging enzyme SOD2	674:719	Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages.
37344477	2	64	theme	colitis	278:284	arg1	ecosystem					286:294	the ulcerative colitis ecosystem	263:294	the ulcerative colitis ecosystem	263:294	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	1	65	theme	inflammatory	153:164	arg1	colitis					132:138	Ulcerative colitis	121:138	Ulcerative colitis	121:138	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	1	65	theme	inflammatory	153:164	arg1	disorder					172:179	a chronic inflammatory bowel disorder	143:179	a chronic inflammatory bowel disorder with cellular heterogeneity	143:207	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	3	66	theme	ulcerative	519:528	arg1	colitis					530:536	the ulcerative colitis	515:536	the ulcerative colitis region	515:543	We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages.
37344477	6	67	theme	mechanism	1100:1108	arg1	components					1084:1093	components	1084:1093	components of a mechanism for the observed macrophage disappearance reaction of resident macrophages	1084:1183	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	6	68	theme	other	1255:1259	arg1	inflammation					1261:1272	other inflammation or infection situations	1255:1296	inflammation	1261:1272	Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations.
37344477	2	69	theme	ulcerative	267:276	arg1	colitis					278:284	the ulcerative colitis	263:284	the ulcerative colitis ecosystem	263:294	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
37344477	1	70	theme	bowel	166:170	arg1	colitis					132:138	Ulcerative colitis	121:138	Ulcerative colitis	121:138	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	1	70	theme	bowel	166:170	arg1	disorder					172:179	a chronic inflammatory bowel disorder	143:179	a chronic inflammatory bowel disorder with cellular heterogeneity	143:207	Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity.
37344477	2	71	dep	composition	228:238	arg1	the					224:226	the	224:226	the	224:226	To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem.
36578441	14	0	theme	trichrome	1765:1773	arg1	staining					1775:1782	Masson trichrome staining	1758:1782	Masson trichrome staining	1758:1782	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	15	1	theme	potential	2046:2054	arg1	healing					2068:2074	a potential bone defect healing	2044:2074	a potential bone defect healing material	2044:2083	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	7	2	theme	biological	904:913	arg1	safety					915:920	its biological safety	900:920	its biological safety	900:920	CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation.
36578441	13	3	theme	strong	1651:1656	arg1	potential					1669:1677	the strong osteogenic potential	1647:1677	the strong osteogenic potential of GA-nCeO2 hydrogel	1647:1698	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	13	4	from	adhesion	1552:1559	arg1	scaffolds					1574:1582	composite scaffolds	1564:1582	composite scaffolds	1564:1582	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	6	5	theme	electron	821:828	arg1	microscope					830:839	the scanning electron microscope	808:839	the scanning electron microscope	808:839	The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope.
36578441	10	6	theme	defect	1102:1107	arg1	model					1109:1113	a bone defect model	1095:1113	a bone defect model	1095:1113	Finally, a bone defect model was made in SD rats, and bone formation in vivo was detected.
36578441	7	7	used	used	883:886	arg2	methods					870:876	live-dead staining methods	851:876	live-dead staining methods	851:876	CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation.
36578441	7	7	used	used	883:886	arg2	CCK8					842:845	CCK8	842:845	CCK8	842:845	CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation.
36578441	12	8	theme	superoxide	1420:1429	arg1	SOD					1442:1444	SOD	1442:1444	SOD	1442:1444	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	12	8	theme	superoxide	1420:1429	arg1	dismutase					1431:1439	superoxide dismutase	1420:1439	superoxide dismutase (SOD)	1420:1445	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	9	9	theme	osteogenic	1040:1049	arg1	genes					1051:1055	osteogenic genes	1040:1055	osteogenic genes	1040:1055	The expression of osteogenic genes was also detected by RT-PCR.
36578441	14	10	theme	staining	1745:1752	arg1	outcomes					1705:1712	The outcomes	1701:1712	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats	1701:1821	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	13	11	theme	gene	1618:1621	arg1	expression					1623:1632	gene expression	1618:1632	gene expression	1618:1632	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	15	12	theme	favorable	1934:1942	arg1	safety					1944:1949	its favorable safety	1930:1949	its favorable safety	1930:1949	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	12	13	theme	simulated	1384:1392	arg1	activity					1401:1408	simulated enzyme activity	1384:1408	simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT)	1384:1464	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	11	14	theme	three-dimensional	1256:1272	arg1	structure					1281:1289	a typical three-dimensional porous structure	1246:1289	a typical three-dimensional porous structure	1246:1289	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	14	15	theme	eosin	1733:1737	arg1	staining					1745:1752	hematoxylin and eosin (H&E) staining	1717:1752	hematoxylin and eosin (H&E) staining	1717:1752	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	14	16	from	outcomes	1705:1712	arg1	model					1806:1810	the femoral defect model	1787:1810	the femoral defect model of SD rats	1787:1821	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	12	17	theme	GA-nCeO2	1336:1343	arg1	hydrogel					1345:1352	The GA-nCeO2 hydrogel	1332:1352	The GA-nCeO2 hydrogel	1332:1352	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	12	17	theme	GA-nCeO2	1336:1343	arg1	endowed					1371:1377	endowed	1371:1377	endowed	1371:1377	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	8	18	from	differentiation	966:980	arg1	stages					1000:1005	early and late stages	985:1005	early and late stages	985:1005	Then the osteogenic differentiation in early and late stages was discussed.
36578441	15	19	theme	defect	2061:2066	arg1	healing					2068:2074	a potential bone defect healing	2044:2074	a potential bone defect healing material	2044:2083	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	14	20	theme	hematoxylin	1717:1727	arg1	staining					1745:1752	hematoxylin and eosin (H&E) staining	1717:1752	hematoxylin and eosin (H&E) staining	1717:1752	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	14	21	theme	bone-promoting	1887:1900	arg1	capacity					1902:1909	bone-promoting capacity	1887:1909	bone-promoting capacity	1887:1909	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	13	22	from	proliferation	1534:1546	arg1	scaffolds					1574:1582	composite scaffolds	1564:1582	composite scaffolds	1564:1582	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	0	23	theme	Osteogenic	67:76	arg1	Nanomaterial					78:89	a Potential Osteogenic Nanomaterial	55:89	a Potential Osteogenic Nanomaterial for Bone Tissue Engineering	55:117	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	0	23	theme	Osteogenic	67:76	arg1	nCeO2					46:50	nCeO2	46:50	nCeO2	46:50	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	13	24	theme	composite	1564:1572	arg1	scaffolds					1574:1582	composite scaffolds	1564:1582	composite scaffolds	1564:1582	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	15	25	theme	bone	2056:2059	arg1	healing					2068:2074	a potential bone defect healing	2044:2074	a potential bone defect healing material	2044:2083	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	3	26	theme	oxide	373:377	arg1	Meanwhile					355:363	Meanwhile	355:363	Meanwhile	355:363	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	3	26	theme	oxide	373:377	arg1	nCeO2					394:398	nCeO2	394:398	nCeO2	394:398	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	3	26	theme	oxide	373:377	arg1	nanoparticles					379:391	cerium oxide nanoparticles	366:391	cerium oxide nanoparticles (nCeO2)	366:399	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	11	27	theme	pore	1303:1306	arg1	ratio					1308:1312	a mean pore ratio	1296:1312	a mean pore ratio of 70.61 ± 1.94%	1296:1329	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	11	28	theme	hydrogel	1218:1225	arg1	scaffold					1227:1234	GA-nCeO2 hydrogel scaffold	1209:1234	GA-nCeO2 hydrogel scaffold	1209:1234	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	0	29	theme	Tissue	100:105	arg1	Engineering					107:117	Bone Tissue Engineering	95:117	Bone Tissue Engineering	95:117	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	5	30	theme	Methods	641:647	arg1	scaffold					667:674	Methods GA-nCeO2 hydrogel scaffold	641:674	Methods GA-nCeO2 hydrogel scaffold	641:674	Methods GA-nCeO2 hydrogel scaffold was prepared by the lyophilized method and characterized.
36578441	11	31	theme	%	1329:1329	arg1	ratio					1308:1312	a mean pore ratio	1296:1312	a mean pore ratio of 70.61 ± 1.94%	1296:1329	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	4	32	theme	hydrogel	499:506	arg1	scaffold					508:515	a porous hydrogel scaffold	490:515	a porous hydrogel scaffold for the application of bone tissue engineering	490:562	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	11	33	theme	±	1323:1323	arg1	%					1329:1329	70.61 ± 1.94%	1317:1329	70.61 ± 1.94%	1317:1329	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	6	34	theme	surface	738:744	arg1	morphology					746:755	surface morphology	738:755	surface morphology	738:755	The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope.
36578441	13	35	theme	cell	1529:1532	arg1	proliferation					1534:1546	superior cell proliferation	1520:1546	superior cell proliferation	1520:1546	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	0	36	theme	Alginate/Gelatin	0:15	arg1	Scaffold					26:33	Alginate/Gelatin Hydrogel Scaffold	0:33	Alginate/Gelatin Hydrogel Scaffold	0:33	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	7	37	theme	live-dead	851:859	arg1	methods					870:876	live-dead staining methods	851:876	live-dead staining methods	851:876	CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation.
36578441	13	38	theme	hydrogel	1691:1698	arg1	potential					1669:1677	the strong osteogenic potential	1647:1677	the strong osteogenic potential of GA-nCeO2 hydrogel	1647:1698	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	1	39	theme	Background	120:129	arg1	Clinicians					131:140	Background Clinicians	120:140	Background Clinicians	120:140	Background Clinicians frequently face difficulties when trying to fix bone abnormalities.
36578441	3	40	theme	enzyme	431:436	arg1	activity					449:456	high antioxidant enzyme simulation activity	414:456	high antioxidant enzyme simulation activity	414:456	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	4	41	theme	GA-nCeO2	585:592	arg1	scaffold					603:610	an appropriate-type GA-nCeO2 hydrogel scaffold	565:610	an appropriate-type GA-nCeO2 hydrogel scaffold	565:610	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	14	42	theme	rats	1818:1821	arg1	model					1806:1810	the femoral defect model	1787:1810	the femoral defect model of SD rats	1787:1821	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	8	43	theme	early	985:989	arg1	stages					1000:1005	early and late stages	985:1005	early and late stages	985:1005	Then the osteogenic differentiation in early and late stages was discussed.
36578441	3	44	theme	high	414:417	arg1	activity					449:456	high antioxidant enzyme simulation activity	414:456	high antioxidant enzyme simulation activity	414:456	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	4	45	theme	bone	540:543	arg1	engineering					552:562	bone tissue engineering	540:562	bone tissue engineering	540:562	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	13	46	theme	osteogenic	1658:1667	arg1	potential					1669:1677	the strong osteogenic potential	1647:1677	the strong osteogenic potential of GA-nCeO2 hydrogel	1647:1698	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	12	47	theme	nCeO2	1488:1492	arg1	addition					1476:1483	the addition	1472:1483	the addition of nCeO2	1472:1492	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	8	48	theme	late	995:998	arg1	stages					1000:1005	early and late stages	985:1005	early and late stages	985:1005	Then the osteogenic differentiation in early and late stages was discussed.
36578441	4	49	theme	engineering	552:562	arg1	application					525:535	the application	521:535	the application of bone tissue engineering	521:562	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	14	50	theme	defect	1799:1804	arg1	model					1806:1810	the femoral defect model	1787:1810	the femoral defect model of SD rats	1787:1821	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	15	51	theme	healing	2068:2074	arg1	material					2076:2083	a potential bone defect healing material	2044:2083	a potential bone defect healing material	2044:2083	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	15	51	theme	healing	2068:2074	arg1	Conclusion					1912:1921	Conclusion	1912:1921	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel	1912:2012	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	15	52	used	used	2036:2039	arg2	Conclusion					1912:1921	Conclusion	1912:1921	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel	1912:2012	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	15	52	used	used	2036:2039	arg2	material					2076:2083	a potential bone defect healing material	2044:2083	a potential bone defect healing material	2044:2083	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	2	53	contain	has	312:314	arg1	it					276:277	it	276:277	it	276:277	Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure.
36578441	2	53	contain	has	312:314	arg2	structure					344:352	a three-dimensional network structure	316:352	a three-dimensional network structure	316:352	Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure.
36578441	14	54	theme	staining	1775:1782	arg1	outcomes					1705:1712	The outcomes	1701:1712	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats	1701:1821	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	5	55	theme	lyophilized	696:706	arg1	method					708:713	the lyophilized method	692:713	the lyophilized method	692:713	Methods GA-nCeO2 hydrogel scaffold was prepared by the lyophilized method and characterized.
36578441	7	56	theme	cell	926:929	arg1	proliferation					931:943	cell proliferation	926:943	cell proliferation	926:943	CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation.
36578441	14	57	theme	Masson	1758:1763	arg1	staining					1775:1782	Masson trichrome staining	1758:1782	Masson trichrome staining	1758:1782	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	13	58	theme	mineralization	1594:1607	arg1	test					1609:1612	mineralization test	1594:1612	mineralization test	1594:1612	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	5	59	theme	hydrogel	658:665	arg1	scaffold					667:674	Methods GA-nCeO2 hydrogel scaffold	641:674	Methods GA-nCeO2 hydrogel scaffold	641:674	Methods GA-nCeO2 hydrogel scaffold was prepared by the lyophilized method and characterized.
36578441	1	60	theme	bone	190:193	arg1	abnormalities					195:207	bone abnormalities	190:207	bone abnormalities	190:207	Background Clinicians frequently face difficulties when trying to fix bone abnormalities.
36578441	12	61	with	endowed	1371:1377	arg1	activity					1401:1408	simulated enzyme activity	1384:1408	simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT)	1384:1464	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	2	62	dep	carrier	260:266	arg1	has					312:314	has	312:314	has a three-dimensional network structure	312:352	Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure.
36578441	2	62	dep	carrier	260:266	arg1	non-toxic					282:290	non-toxic	282:290	non-toxic	282:290	Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure.
36578441	2	63	theme	three-dimensional	318:334	arg1	structure					344:352	a three-dimensional network structure	316:352	a three-dimensional network structure	316:352	Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure.
36578441	12	64	theme	enzyme	1394:1399	arg1	activity					1401:1408	simulated enzyme activity	1384:1408	simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT)	1384:1464	The GA-nCeO2 hydrogel was successfully endowed with simulated enzyme activity including superoxide dismutase (SOD) and catalase (CAT) after the addition of nCeO2.
36578441	6	65	theme	scanning	812:819	arg1	microscope					830:839	the scanning electron microscope	808:839	the scanning electron microscope	808:839	The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope.
36578441	9	66	theme	genes	1051:1055	arg1	expression					1026:1035	The expression	1022:1035	The expression of osteogenic genes	1022:1055	The expression of osteogenic genes was also detected by RT-PCR.
36578441	15	67	theme	Due	1923:1925	arg1	material					2076:2083	a potential bone defect healing material	2044:2083	a potential bone defect healing material	2044:2083	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	15	67	theme	Due	1923:1925	arg1	Conclusion					1912:1921	Conclusion	1912:1921	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel	1912:2012	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	10	68	theme	bone	1097:1100	arg1	model					1109:1113	a bone defect model	1095:1113	a bone defect model	1095:1113	Finally, a bone defect model was made in SD rats, and bone formation in vivo was detected.
36578441	2	69	dep	non-toxic	282:290	arg1	biodegradable					293:305	biodegradable	293:305	biodegradable	293:305	Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure.
36578441	0	70	theme	Bone	95:98	arg1	Engineering					107:117	Bone Tissue Engineering	95:117	Bone Tissue Engineering	95:117	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	14	71	theme	favorable	1856:1864	arg1	biocompatibility					1866:1881	the scaffold's favorable biocompatibility	1841:1881	the scaffold's favorable biocompatibility	1841:1881	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	4	72	theme	porous	492:497	arg1	scaffold					508:515	a porous hydrogel scaffold	490:515	a porous hydrogel scaffold for the application of bone tissue engineering	490:562	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	15	73	theme	GA-nCeO2	1996:2003	arg1	hydrogel					2005:2012	GA-nCeO2 hydrogel	1996:2012	GA-nCeO2 hydrogel	1996:2012	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	6	74	theme	cell	761:764	arg1	adhesion					766:773	cell adhesion	761:773	cell adhesion	761:773	The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope.
36578441	5	75	theme	GA-nCeO2	649:656	arg1	scaffold					667:674	Methods GA-nCeO2 hydrogel scaffold	641:674	Methods GA-nCeO2 hydrogel scaffold	641:674	Methods GA-nCeO2 hydrogel scaffold was prepared by the lyophilized method and characterized.
36578441	3	76	theme	cerium	366:371	arg1	Meanwhile					355:363	Meanwhile	355:363	Meanwhile	355:363	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	3	76	theme	cerium	366:371	arg1	nCeO2					394:398	nCeO2	394:398	nCeO2	394:398	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	3	76	theme	cerium	366:371	arg1	nanoparticles					379:391	cerium oxide nanoparticles	366:391	cerium oxide nanoparticles (nCeO2)	366:399	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	6	77	dep	morphology	746:755	arg1	The					734:736	The	734:736	The	734:736	The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope.
36578441	13	78	theme	superior	1520:1527	arg1	proliferation					1534:1546	superior cell proliferation	1520:1546	superior cell proliferation	1520:1546	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	0	79	theme	Hydrogel	17:24	arg1	Scaffold					26:33	Alginate/Gelatin Hydrogel Scaffold	0:33	Alginate/Gelatin Hydrogel Scaffold	0:33	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	10	80	theme	SD	1127:1128	arg1	rats					1130:1133	SD rats	1127:1133	SD rats	1127:1133	Finally, a bone defect model was made in SD rats, and bone formation in vivo was detected.
36578441	4	81	theme	appropriate-type	568:583	arg1	scaffold					603:610	an appropriate-type GA-nCeO2 hydrogel scaffold	565:610	an appropriate-type GA-nCeO2 hydrogel scaffold	565:610	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	15	82	theme	bone	1971:1974	arg1	formation					1976:1984	bone formation	1971:1984	bone formation property	1971:1993	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	15	83	theme	formation	1976:1984	arg1	property					1986:1993	bone formation property	1971:1993	bone formation property	1971:1993	Conclusion Due to its favorable safety, degradability, and bone formation property, GA-nCeO2 hydrogel was anticipated to be used as a potential bone defect healing material.
36578441	7	84	theme	staining	861:868	arg1	methods					870:876	live-dead staining methods	851:876	live-dead staining methods	851:876	CCK8 and live-dead staining methods were used to evaluate its biological safety and cell proliferation.
36578441	11	85	theme	GA-nCeO2	1209:1216	arg1	scaffold					1227:1234	GA-nCeO2 hydrogel scaffold	1209:1234	GA-nCeO2 hydrogel scaffold	1209:1234	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	4	86	theme	hydrogel	594:601	arg1	scaffold					603:610	an appropriate-type GA-nCeO2 hydrogel scaffold	565:610	an appropriate-type GA-nCeO2 hydrogel scaffold	565:610	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	11	87	theme	porous	1274:1279	arg1	structure					1281:1289	a typical three-dimensional porous structure	1246:1289	a typical three-dimensional porous structure	1246:1289	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	10	88	theme	bone	1140:1143	arg1	formation					1145:1153	bone formation	1140:1153	bone formation in vivo	1140:1161	Finally, a bone defect model was made in SD rats, and bone formation in vivo was detected.
36578441	8	89	theme	osteogenic	955:964	arg1	differentiation					966:980	Then the osteogenic differentiation	946:980	Then the osteogenic differentiation in early and late stages	946:1005	Then the osteogenic differentiation in early and late stages was discussed.
36578441	3	90	theme	simulation	438:447	arg1	activity					449:456	high antioxidant enzyme simulation activity	414:456	high antioxidant enzyme simulation activity	414:456	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	4	91	theme	tissue	545:550	arg1	engineering					552:562	bone tissue engineering	540:562	bone tissue engineering	540:562	Therefore, in order to develop a porous hydrogel scaffold for the application of bone tissue engineering, an appropriate-type GA-nCeO2 hydrogel scaffold was developed and evaluated.
36578441	11	92	theme	typical	1248:1254	arg1	structure					1281:1289	a typical three-dimensional porous structure	1246:1289	a typical three-dimensional porous structure	1246:1289	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	11	93	dep	Results	1177:1183	arg1	showed					1197:1202	showed	1197:1202	showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%	1197:1329	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	0	94	theme	Potential	57:65	arg1	Nanomaterial					78:89	a Potential Osteogenic Nanomaterial	55:89	a Potential Osteogenic Nanomaterial for Bone Tissue Engineering	55:117	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	0	94	theme	Potential	57:65	arg1	nCeO2					46:50	nCeO2	46:50	nCeO2	46:50	Alginate/Gelatin Hydrogel Scaffold Containing nCeO2 as a Potential Osteogenic Nanomaterial for Bone Tissue Engineering.
36578441	14	95	theme	SD	1815:1816	arg1	rats					1818:1821	SD rats	1815:1821	SD rats	1815:1821	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36578441	3	96	theme	antioxidant	419:429	arg1	activity					449:456	high antioxidant enzyme simulation activity	414:456	high antioxidant enzyme simulation activity	414:456	Meanwhile, cerium oxide nanoparticles (nCeO2) demonstrated high antioxidant enzyme simulation activity.
36578441	11	97	theme	mean	1298:1301	arg1	ratio					1308:1312	a mean pore ratio	1296:1312	a mean pore ratio of 70.61 ± 1.94%	1296:1329	Results The results showed that GA-nCeO2 hydrogel scaffold exhibited a typical three-dimensional porous structure with a mean pore ratio of 70.61 ± 1.94%.
36578441	13	98	theme	GA-nCeO2	1682:1689	arg1	hydrogel					1691:1698	GA-nCeO2 hydrogel	1682:1698	GA-nCeO2 hydrogel	1682:1698	Osteoblasts demonstrated superior cell proliferation and adhesion on composite scaffolds, and both mineralization test and gene expression demonstrated the strong osteogenic potential of GA-nCeO2 hydrogel.
36578441	6	99	theme	scaffold	782:789	arg1	morphology					746:755	surface morphology	738:755	surface morphology	738:755	The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope.
36578441	6	99	theme	scaffold	782:789	arg1	adhesion					766:773	cell adhesion	761:773	cell adhesion	761:773	The surface morphology and cell adhesion of the scaffold were observed by the scanning electron microscope.
36578441	2	100	theme	network	336:342	arg1	structure					344:352	a three-dimensional network structure	316:352	a three-dimensional network structure	316:352	Gelatin-Alginate (GA) is frequently employed as a carrier because it is non-toxic, biodegradable, and has a three-dimensional network structure.
36578441	14	101	theme	femoral	1791:1797	arg1	model					1806:1810	the femoral defect model	1787:1810	the femoral defect model of SD rats	1787:1821	The outcomes of hematoxylin and eosin (H&E) staining and Masson trichrome staining in the femoral defect model of SD rats further supported the scaffold's favorable biocompatibility and bone-promoting capacity.
36049623	12	0	theme	Gaussia	2010:2016	arg1	HuCol2gLuc					2044:2053	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	14	1	theme	reporter	2194:2201	arg1	system					2203:2208	This reporter system	2189:2208	This reporter system	2189:2208	This reporter system streamlines the biomaterial optimization process before 3D bioprinting.
36049623	11	2	theme	cartilage	1818:1826	arg1	development					1828:1838	articular cartilage development	1808:1838	articular cartilage development	1808:1838	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.
36049623	12	3	theme	primary	1967:1973	arg1	collagen					2001:2008	a primary human chondrocyte type II collagen	1965:2008	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	16	4	theme	bioprinted	2430:2439	arg1	construct					2441:2449	a 3D bioprinted construct	2425:2449	a 3D bioprinted construct	2425:2449	And for the first time, we quantified chondrocyte mobility in a 3D bioprinted construct.
36049623	17	5	theme	storage	2517:2523	arg1	modulus					2525:2531	storage modulus	2517:2531	storage modulus	2517:2531	Together these results indicate that a biomaterial with a higher storage modulus and less cell mobility, improves chondrogenesis.
36049623	13	6	dep	non-destructive	2062:2076	arg1	high-throughput					2079:2093	high-throughput	2079:2093	high-throughput	2079:2093	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	15	7	used	used	2290:2293	arg2	We					2282:2283	We	2282:2283	We	2282:2283	We also used it to determine the level of stiffness required for chondrogenesis.
36049623	1	8	theme	well-established	261:276	arg1	characteristics					287:301	individual well-established material characteristics	250:301	individual well-established material characteristics	250:301	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	8	9	theme	storage	1154:1160	arg1	moduli					1162:1167	biomaterial storage moduli	1142:1167	biomaterial storage moduli impact cell mobility	1142:1188	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	12	10	theme	acid	1915:1918	arg1	methacrylate					1920:1931	hyaluronic acid methacrylate	1904:1931	hyaluronic acid methacrylate for chondrogenesis	1904:1950	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	17	11	dep	higher	2510:2515	arg1	modulus					2525:2531	storage modulus	2517:2531	storage modulus	2517:2531	Together these results indicate that a biomaterial with a higher storage modulus and less cell mobility, improves chondrogenesis.
36049623	8	12	theme	fluorescent	1240:1250	arg1	GFP					1261:1263	GFP	1261:1263	GFP	1261:1263	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	8	12	theme	fluorescent	1240:1250	arg1	protein					1252:1258	green fluorescent protein	1234:1258	green fluorescent protein (GFP)	1234:1264	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	10	13	theme	3D	1539:1540	arg1	bioprinting					1542:1552	3D bioprinting	1539:1552	3D bioprinting with HuCol2gLuc cells	1539:1574	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	2	14	theme	ideal	319:323	arg1	combination					325:335	an ideal combination	316:335	an ideal combination of GelMA and HAMA for chondrogenesis	316:372	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	7	15	theme	HAMA	1047:1050	arg1	ratios					1052:1057	the same GelMA:HAMA ratios	1032:1057	the same GelMA:HAMA ratios	1032:1057	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	8	16	theme	human	1191:1195	arg1	chondrocytes					1205:1216	human primary chondrocytes	1191:1216	human primary chondrocytes transduced with green fluorescent protein (GFP)	1191:1264	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	1	17	theme	Gelatin	123:129	arg1	methacrylate					131:142	Gelatin methacrylate	123:142	Gelatin methacrylate (GelMA)	123:150	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	1	17	theme	Gelatin	123:129	arg1	GelMA					145:149	GelMA	145:149	GelMA	145:149	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	1	18	used	used	207:210	arg2	HAMA					186:189	HAMA	186:189	HAMA	186:189	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	1	18	used	used	207:210	arg2	GelMA					145:149	GelMA	145:149	GelMA	145:149	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	1	18	used	used	207:210	arg2	methacrylate					172:183	hyaluronic acid methacrylate	156:183	hyaluronic acid methacrylate (HAMA)	156:190	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	1	18	used	used	207:210	arg2	methacrylate					131:142	Gelatin methacrylate	123:142	Gelatin methacrylate (GelMA)	123:150	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	3	19	used	used	589:592	arg2	luciferase					547:556	Gaussia luciferase	539:556	Gaussia luciferase	539:556	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
36049623	3	19	used	used	589:592	arg2	proxy					599:603	a proxy	597:603	a proxy for measuring type II collagen production	597:645	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
36049623	7	20	theme	GelMA	1041:1045	arg1	ratios					1052:1057	the same GelMA:HAMA ratios	1032:1057	the same GelMA:HAMA ratios	1032:1057	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	13	21	theme	secreted	2108:2115	arg1	luciferase					2125:2134	a secreted Gaussia luciferase	2106:2134	a secreted Gaussia luciferase	2106:2134	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	13	21	theme	secreted	2108:2115	arg1	proxy					2141:2145	a proxy	2139:2145	a proxy for temporal type II collagen production	2139:2186	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	5	22	theme	biomaterial	837:847	arg1	optimization					849:860	biomaterial optimization	837:860	biomaterial optimization	837:860	This method is efficient, saving on time and materials, resulting in a streamlined process of biomaterial optimization.
36049623	9	23	with	material	1490:1497	arg1	mobility					1514:1521	more cell mobility	1504:1521	more cell mobility	1504:1521	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	11	24	theme	3D	1744:1745	arg1	bioprinting					1747:1757	3D bioprinting	1744:1757	3D bioprinting	1744:1757	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.
36049623	13	25	theme	non-destructive	2062:2076	arg1	assay					2095:2099	This non-destructive, high-throughput assay	2057:2099	This non-destructive, high-throughput assay	2057:2099	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	9	26	theme	type	1445:1448	arg1	collagen					1453:1460	type II collagen	1445:1460	higher type II collagen expression	1438:1471	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	4	27	theme	3D	727:728	arg1	bioprinting					730:740	3D bioprinting	727:740	3D bioprinting	727:740	GelMA:HAMA ratios were screened using the reporter system before proceeding to 3D bioprinting.
36049623	10	28	dep	HAMA	1641:1644	arg1	time					1669:1672	time	1669:1672	time	1669:1672	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	15	29	theme	stiffness	2324:2332	arg1	level					2315:2319	the level	2311:2319	the level of stiffness required for chondrogenesis	2311:2360	We also used it to determine the level of stiffness required for chondrogenesis.
36049623	3	30	theme	collagen	627:634	arg1	production					636:645	type II collagen production	619:645	type II collagen production	619:645	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
36049623	7	31	theme	Storage	966:972	arg1	moduli					974:979	Storage moduli	966:979	Storage moduli	966:979	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	9	32	theme	collagen	1453:1460	arg1	expression					1462:1471	higher type II collagen expression	1438:1471	higher type II collagen expression	1438:1471	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	8	33	theme	1:1	1295:1297	arg1	ratios					1306:1311	either 1:1 or 2:1 ratios	1288:1311	either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa	1288:1352	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	1	34	theme	individual	250:259	arg1	characteristics					287:301	individual well-established material characteristics	250:301	individual well-established material characteristics	250:301	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	3	35	theme	type	619:622	arg1	collagen					627:634	type II collagen	619:634	type II collagen production	619:645	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
36049623	11	36	theme	ideal	1774:1778	arg1	biomaterials					1780:1791	ideal biomaterials	1774:1791	ideal biomaterials that stimulate articular cartilage development	1774:1838	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.
36049623	8	37	theme	2:1	1302:1304	arg1	ratios					1306:1311	either 1:1 or 2:1 ratios	1288:1311	either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa	1288:1352	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	0	38	theme	constructs	78:87	arg1	properties					50:59	decisive properties	41:59	decisive properties of 3D bioprinted constructs	41:87	Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation.
36049623	1	39	theme	hyaluronic	156:165	arg1	HAMA					186:189	HAMA	186:189	HAMA	186:189	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	1	39	theme	hyaluronic	156:165	arg1	methacrylate					172:183	hyaluronic acid methacrylate	156:183	hyaluronic acid methacrylate (HAMA)	156:190	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	0	40	theme	type	93:96	arg1	collagen					101:108	type II collagen	93:108	type II collagen stimulation	93:120	Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation.
36049623	12	41	theme	gelatin	1879:1885	arg1	methacrylate					1887:1898	gelatin methacrylate	1879:1898	gelatin methacrylate	1879:1898	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	3	42	dep	non-destructive	490:504	arg1	temporal					523:530	temporal	523:530	temporal	523:530	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
36049623	9	43	contain	had	1434:1436	arg1	mobility					1396:1403	reduced cell mobility	1383:1403	reduced cell mobility	1383:1403	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	9	43	contain	had	1434:1436	arg2	expression					1462:1471	higher type II collagen expression	1438:1471	higher type II collagen expression	1438:1471	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	17	44	theme	cell	2542:2545	arg1	mobility					2547:2554	a higher storage modulus and less cell mobility	2508:2554	a higher storage modulus and less cell mobility	2508:2554	Together these results indicate that a biomaterial with a higher storage modulus and less cell mobility, improves chondrogenesis.
36049623	10	45	theme	optimal	1606:1612	arg1	2:1					1627:1629	2:1	1627:1629	2:1	1627:1629	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	10	45	theme	optimal	1606:1612	arg1	combination					1614:1624	an optimal combination	1603:1624	an optimal combination (2:1) of GelMA	1603:1639	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	9	46	theme	cell	1391:1394	arg1	mobility					1396:1403	reduced cell mobility	1383:1403	reduced cell mobility	1383:1403	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	12	47	theme	chondrocyte	1981:1991	arg1	collagen					2001:2008	a primary human chondrocyte type II collagen	1965:2008	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	8	48	theme	32kPa	1336:1340	arg1	moduli					1326:1331	storage moduli	1318:1331	storage moduli of 32kPa and 57.9kPa	1318:1352	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	14	49	theme	3D	2266:2267	arg1	bioprinting					2269:2279	3D bioprinting	2266:2279	3D bioprinting	2266:2279	This reporter system streamlines the biomaterial optimization process before 3D bioprinting.
36049623	1	50	theme	material	278:285	arg1	characteristics					287:301	individual well-established material characteristics	250:301	individual well-established material characteristics	250:301	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	8	51	theme	57.9kPa	1346:1352	arg1	moduli					1326:1331	storage moduli	1318:1331	storage moduli of 32kPa and 57.9kPa	1318:1352	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	2	52	theme	reporter	436:443	arg1	HuCol2gLuc					453:462	HuCol2gLuc	453:462	HuCol2gLuc	453:462	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	2	52	theme	reporter	436:443	arg1	system					445:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system	375:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc)	375:463	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	12	53	theme	reporter	2029:2036	arg1	HuCol2gLuc					2044:2053	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	14	54	theme	biomaterial	2226:2236	arg1	process					2251:2257	the biomaterial optimization process	2222:2257	the biomaterial optimization process	2222:2257	This reporter system streamlines the biomaterial optimization process before 3D bioprinting.
36049623	2	55	theme	COL2A1-Gaussia	410:423	arg1	HuCol2gLuc					453:462	HuCol2gLuc	453:462	HuCol2gLuc	453:462	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	2	55	theme	COL2A1-Gaussia	410:423	arg1	system					445:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system	375:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc)	375:463	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	5	56	theme	optimization	849:860	arg1	process					826:832	a streamlined process	812:832	a streamlined process of biomaterial optimization	812:860	This method is efficient, saving on time and materials, resulting in a streamlined process of biomaterial optimization.
36049623	13	57	theme	collagen	2168:2175	arg1	production					2177:2186	temporal type II collagen production	2151:2186	temporal type II collagen production	2151:2186	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	12	58	theme	human	1975:1979	arg1	collagen					2001:2008	a primary human chondrocyte type II collagen	1965:2008	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	0	59	theme	3D	64:65	arg1	constructs					78:87	3D bioprinted constructs	64:87	3D bioprinted constructs	64:87	Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation.
36049623	16	60	theme	first	2375:2379	arg1	time					2381:2384	the first time	2371:2384	the first time	2371:2384	And for the first time, we quantified chondrocyte mobility in a 3D bioprinted construct.
36049623	2	61	theme	HAMA	350:353	arg1	combination					325:335	an ideal combination	316:335	an ideal combination of GelMA and HAMA for chondrogenesis	316:372	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	7	62	theme	∼30kPa	1118:1123	arg1	chondrogenesis					1100:1113	chondrogenesis	1100:1113	chondrogenesis of ∼30kPa	1100:1123	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	10	63	with	bioprinting	1542:1552	arg1	cells					1570:1574	HuCol2gLuc cells	1559:1574	HuCol2gLuc cells	1559:1574	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	10	64	dep	identified	1592:1601	arg1	photo-crosslinking					1650:1667	photo-crosslinking	1650:1667	photo-crosslinking	1650:1667	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	10	64	dep	identified	1592:1601	arg1	38s					1675:1677	38s	1675:1677	38s	1675:1677	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	10	64	dep	identified	1592:1601	arg1	HAMA					1641:1644	HAMA	1641:1644	HAMA	1641:1644	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	10	65	theme	GelMA	1635:1639	arg1	2:1					1627:1629	2:1	1627:1629	2:1	1627:1629	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	10	65	theme	GelMA	1635:1639	arg1	combination					1614:1624	an optimal combination	1603:1624	an optimal combination (2:1) of GelMA	1603:1639	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	2	66	theme	chondrocyte	398:408	arg1	HuCol2gLuc					453:462	HuCol2gLuc	453:462	HuCol2gLuc	453:462	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	2	66	theme	chondrocyte	398:408	arg1	system					445:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system	375:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc)	375:463	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	1	67	theme	3D	229:230	arg1	bioprinting					232:242	3D bioprinting	229:242	3D bioprinting	229:242	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	12	68	theme	type	1993:1996	arg1	collagen					2001:2008	a primary human chondrocyte type II collagen	1965:2008	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	16	69	theme	3D	2427:2428	arg1	construct					2441:2449	a 3D bioprinted construct	2425:2449	a 3D bioprinted construct	2425:2449	And for the first time, we quantified chondrocyte mobility in a 3D bioprinted construct.
36049623	0	70	dep	Biomaterial	0:10	arg1	composition					12:22	composition	12:22	composition	12:22	Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation.
36049623	9	71	from	mobility	1396:1403	arg1	biomaterial					1421:1431	the stiffer biomaterial	1409:1431	the stiffer biomaterial	1409:1431	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	11	72	theme	articular	1808:1816	arg1	development					1828:1838	articular cartilage development	1808:1838	articular cartilage development	1808:1838	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.
36049623	12	73	theme	collagen	2001:2008	arg1	HuCol2gLuc					2044:2053	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	6	74	theme	HAMA	904:907	arg1	addition					892:899	the addition	888:899	the addition of HAMA to GelMA	888:916	The screen revealed that the addition of HAMA to GelMA improved chondrogenesis over GelMA (15%) alone.
36049623	17	75	theme	higher	2510:2515	arg1	mobility					2547:2554	a higher storage modulus and less cell mobility	2508:2554	a higher storage modulus and less cell mobility	2508:2554	Together these results indicate that a biomaterial with a higher storage modulus and less cell mobility, improves chondrogenesis.
36049623	7	76	theme	initial	1078:1084	arg1	threshold					1086:1094	an initial threshold	1075:1094	an initial threshold for chondrogenesis of ∼30kPa	1075:1123	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	9	77	theme	reduced	1383:1389	arg1	mobility					1396:1403	reduced cell mobility	1383:1403	reduced cell mobility	1383:1403	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	17	78	with	biomaterial	2491:2501	arg1	mobility					2547:2554	a higher storage modulus and less cell mobility	2508:2554	a higher storage modulus and less cell mobility	2508:2554	Together these results indicate that a biomaterial with a higher storage modulus and less cell mobility, improves chondrogenesis.
36049623	1	79	theme	acid	167:170	arg1	HAMA					186:189	HAMA	186:189	HAMA	186:189	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	1	79	theme	acid	167:170	arg1	methacrylate					172:183	hyaluronic acid methacrylate	156:183	hyaluronic acid methacrylate (HAMA)	156:190	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	8	80	theme	biomaterial	1142:1152	arg1	moduli					1162:1167	biomaterial storage moduli	1142:1167	biomaterial storage moduli impact cell mobility	1142:1188	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	4	81	theme	HAMA	654:657	arg1	ratios					659:664	GelMA:HAMA ratios	648:664	GelMA:HAMA ratios	648:664	GelMA:HAMA ratios were screened using the reporter system before proceeding to 3D bioprinting.
36049623	7	82	theme	ratios	1052:1057	arg1	analysis					1020:1027	dynamic mechanical analysis	1001:1027	dynamic mechanical analysis of the same GelMA:HAMA ratios	1001:1057	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	8	83	theme	green	1234:1238	arg1	GFP					1261:1263	GFP	1261:1263	GFP	1261:1263	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	8	83	theme	green	1234:1238	arg1	protein					1252:1258	green fluorescent protein	1234:1258	green fluorescent protein (GFP)	1234:1264	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	9	84	theme	stiffer	1413:1419	arg1	biomaterial					1421:1431	the stiffer biomaterial	1409:1431	the stiffer biomaterial	1409:1431	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	8	85	theme	cell	1176:1179	arg1	mobility					1181:1188	cell mobility	1176:1188	cell mobility	1176:1188	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	12	86	theme	cell	2038:2041	arg1	HuCol2gLuc					2044:2053	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	7	87	theme	same	1036:1039	arg1	ratios					1052:1057	the same GelMA:HAMA ratios	1032:1057	the same GelMA:HAMA ratios	1032:1057	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	5	88	dep	efficient	758:766	arg1	saving					769:774	saving	769:774	saving	769:774	This method is efficient, saving on time and materials, resulting in a streamlined process of biomaterial optimization.
36049623	4	89	theme	GelMA	648:652	arg1	ratios					659:664	GelMA:HAMA ratios	648:664	GelMA:HAMA ratios	648:664	GelMA:HAMA ratios were screened using the reporter system before proceeding to 3D bioprinting.
36049623	10	90	theme	HuCol2gLuc	1559:1568	arg1	cells					1570:1574	HuCol2gLuc cells	1559:1574	HuCol2gLuc cells	1559:1574	Finally, after 3D bioprinting with HuCol2gLuc cells we successfully identified an optimal combination (2:1) of GelMA:HAMA and photo-crosslinking time (38s) for chondrogenesis.
36049623	13	91	theme	Gaussia	2117:2123	arg1	luciferase					2125:2134	a secreted Gaussia luciferase	2106:2134	a secreted Gaussia luciferase	2106:2134	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	13	91	theme	Gaussia	2117:2123	arg1	proxy					2141:2145	a proxy	2139:2145	a proxy for temporal type II collagen production	2139:2186	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	7	92	theme	dynamic	1001:1007	arg1	analysis					1020:1027	dynamic mechanical analysis	1001:1027	dynamic mechanical analysis of the same GelMA:HAMA ratios	1001:1057	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	9	93	theme	higher	1438:1443	arg1	expression					1462:1471	higher type II collagen expression	1438:1471	higher type II collagen expression	1438:1471	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	8	94	theme	primary	1197:1203	arg1	chondrocytes					1205:1216	human primary chondrocytes	1191:1216	human primary chondrocytes transduced with green fluorescent protein (GFP)	1191:1264	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	11	95	theme	bioprinting	1747:1757	arg1	challenge					1731:1739	One challenge	1727:1739	One challenge of 3D bioprinting	1727:1757	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.
36049623	7	96	theme	mechanical	1009:1018	arg1	analysis					1020:1027	dynamic mechanical analysis	1001:1027	dynamic mechanical analysis of the same GelMA:HAMA ratios	1001:1057	Storage moduli were measured using dynamic mechanical analysis of the same GelMA:HAMA ratios and established an initial threshold for chondrogenesis of ∼30kPa.
36049623	11	97	dep	STATEMENT	1700:1708	arg1	is					1759:1760	is	1759:1760	is identifying ideal biomaterials that stimulate articular cartilage development	1759:1838	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.
36049623	2	98	theme	GelMA	340:344	arg1	combination					325:335	an ideal combination	316:335	an ideal combination of GelMA and HAMA for chondrogenesis	316:372	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	0	99	theme	bioprinted	67:76	arg1	constructs					78:87	3D bioprinted constructs	64:87	3D bioprinted constructs	64:87	Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation.
36049623	5	100	theme	streamlined	814:824	arg1	process					826:832	a streamlined process	812:832	a streamlined process of biomaterial optimization	812:860	This method is efficient, saving on time and materials, resulting in a streamlined process of biomaterial optimization.
36049623	12	101	theme	luciferase	2018:2027	arg1	HuCol2gLuc					2044:2053	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc)	1965:2054	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	8	102	with	ratios	1306:1311	arg1	moduli					1326:1331	storage moduli	1318:1331	storage moduli of 32kPa and 57.9kPa	1318:1352	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	4	103	theme	reporter	690:697	arg1	system					699:704	the reporter system	686:704	the reporter system	686:704	GelMA:HAMA ratios were screened using the reporter system before proceeding to 3D bioprinting.
36049623	11	104	theme	SIGNIFICANCE	1713:1724	arg1	STATEMENT					1700:1708	STATEMENT	1700:1708	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.	1700:1839	STATEMENT OF SIGNIFICANCE: One challenge of 3D bioprinting is identifying ideal biomaterials that stimulate articular cartilage development.
36049623	12	105	theme	methacrylate	1887:1898	arg1	methacrylate					1920:1931	hyaluronic acid methacrylate	1904:1931	hyaluronic acid methacrylate for chondrogenesis	1904:1950	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	12	105	theme	methacrylate	1887:1898	arg1	combination					1864:1874	an optimal combination	1853:1874	an optimal combination of gelatin methacrylate	1853:1898	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	9	106	theme	softer	1483:1488	arg1	material					1490:1497	the softer material	1479:1497	the softer material with more cell mobility	1479:1521	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	17	107	theme	less	2537:2540	arg1	mobility					2547:2554	a higher storage modulus and less cell mobility	2508:2554	a higher storage modulus and less cell mobility	2508:2554	Together these results indicate that a biomaterial with a higher storage modulus and less cell mobility, improves chondrogenesis.
36049623	12	108	theme	hyaluronic	1904:1913	arg1	methacrylate					1920:1931	hyaluronic acid methacrylate	1904:1931	hyaluronic acid methacrylate for chondrogenesis	1904:1950	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	8	109	theme	storage	1318:1324	arg1	moduli					1326:1331	storage moduli	1318:1331	storage moduli of 32kPa and 57.9kPa	1318:1352	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	12	110	theme	optimal	1856:1862	arg1	combination					1864:1874	an optimal combination	1853:1874	an optimal combination of gelatin methacrylate	1853:1898	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	9	111	theme	cell	1509:1512	arg1	mobility					1514:1521	more cell mobility	1504:1521	more cell mobility	1504:1521	We found that reduced cell mobility, in the stiffer biomaterial, had higher type II collagen expression, than the softer material with more cell mobility.
36049623	12	112	dep	developed	1955:1963	arg1	identify					1844:1851	identify	1844:1851	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis	1841:1950	To identify an optimal combination of gelatin methacrylate and hyaluronic acid methacrylate for chondrogenesis we developed a primary human chondrocyte type II collagen Gaussia luciferase reporter cell (HuCol2gLuc).
36049623	2	113	theme	novel	377:381	arg1	HuCol2gLuc					453:462	HuCol2gLuc	453:462	HuCol2gLuc	453:462	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	2	113	theme	novel	377:381	arg1	system					445:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system	375:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc)	375:463	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	14	114	theme	optimization	2238:2249	arg1	process					2251:2257	the biomaterial optimization process	2222:2257	the biomaterial optimization process	2222:2257	This reporter system streamlines the biomaterial optimization process before 3D bioprinting.
36049623	2	115	theme	luciferase	425:434	arg1	HuCol2gLuc					453:462	HuCol2gLuc	453:462	HuCol2gLuc	453:462	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	2	115	theme	luciferase	425:434	arg1	system					445:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system	375:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc)	375:463	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	0	116	theme	decisive	41:48	arg1	properties					50:59	decisive properties	41:59	decisive properties of 3D bioprinted constructs	41:87	Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation.
36049623	2	117	theme	human	392:396	arg1	HuCol2gLuc					453:462	HuCol2gLuc	453:462	HuCol2gLuc	453:462	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	2	117	theme	human	392:396	arg1	system					445:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system	375:450	a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc)	375:463	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	13	118	theme	type	2160:2163	arg1	collagen					2168:2175	temporal type II collagen	2151:2175	temporal type II collagen production	2151:2186	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	0	119	theme	collagen	101:108	arg1	stimulation					110:120	type II collagen stimulation	93:120	type II collagen stimulation	93:120	Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation.
36049623	1	120	with	biomaterials	212:223	arg1	characteristics					287:301	individual well-established material characteristics	250:301	individual well-established material characteristics	250:301	Gelatin methacrylate (GelMA) and hyaluronic acid methacrylate (HAMA) are frequently used biomaterials for 3D bioprinting, with individual well-established material characteristics.
36049623	8	121	dep	moduli	1162:1167	arg1	impact					1169:1174	impact	1169:1174	impact	1169:1174	To determine if biomaterial storage moduli impact cell mobility, human primary chondrocytes transduced with green fluorescent protein (GFP) were 3D bioprinted in either 1:1 or 2:1 ratios with storage moduli of 32kPa and 57.9kPa, respectively.
36049623	2	122	dep	novel	377:381	arg1	primary					384:390	primary	384:390	primary	384:390	To identify an ideal combination of GelMA and HAMA for chondrogenesis, a novel, primary human chondrocyte COL2A1-Gaussia luciferase reporter system (HuCol2gLuc) was developed.
36049623	16	123	theme	chondrocyte	2401:2411	arg1	mobility					2413:2420	chondrocyte mobility	2401:2420	chondrocyte mobility	2401:2420	And for the first time, we quantified chondrocyte mobility in a 3D bioprinted construct.
36049623	3	124	theme	non-destructive	490:504	arg1	assay					532:536	this non-destructive, high-throughput temporal assay	485:536	this non-destructive, high-throughput temporal assay	485:536	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
36049623	13	125	theme	temporal	2151:2158	arg1	collagen					2168:2175	temporal type II collagen	2151:2175	temporal type II collagen production	2151:2186	This non-destructive, high-throughput assay uses a secreted Gaussia luciferase as a proxy for temporal type II collagen production.
36049623	3	126	theme	Gaussia	539:545	arg1	proxy					599:603	a proxy	597:603	a proxy for measuring type II collagen production	597:645	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
36049623	3	126	theme	Gaussia	539:545	arg1	luciferase					547:556	Gaussia luciferase	539:556	Gaussia luciferase	539:556	With this non-destructive, high-throughput temporal assay, Gaussia luciferase is secreted from the cells and used as a proxy for measuring type II collagen production.
35306095	8	0	theme	depression-like	1132:1146	arg1	behaviors					1148:1156	LPS-induced depression-like behaviors	1120:1156	LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test	1120:1258	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	5	1	dep	injection	611:619	arg1	induce					624:629	induce	624:629	to induce depression-like symptoms	621:654	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	9	2	theme	lesser	1415:1420	arg1	extent					1422:1427	a lesser extent	1413:1427	a lesser extent	1413:1427	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	6	3	theme	open	812:815	arg1	field					817:821	open field	812:821	open field	812:821	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	5	4	theme	C57BL/6	510:516	arg1	mice					518:521	C57BL/6 mice	510:521	C57BL/6 mice	510:521	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	8	5	theme	decreased	1177:1185	arg1	preference					1195:1204	decreased sucrose preference	1177:1204	decreased sucrose preference	1177:1204	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	5	6	dep	treated	566:572	arg1	followed					657:664	followed	657:664	followed by oral administration of LL95 for one week (1 × 109 CFU/mouse)	657:728	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	8	7	theme	swim	1250:1253	arg1	test					1255:1258	the forced swim test	1239:1258	the forced swim test	1239:1258	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	11	8	theme	microbiota	1815:1824	arg1	content					1796:1802	the LAB content	1788:1802	the LAB content of the gut microbiota	1788:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	5	9	theme	oral	669:672	arg1	administration					674:687	oral administration	669:687	oral administration of LL95 for one week (1 × 109 CFU/mouse)	669:728	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	6	10	theme	behavioral	778:787	arg1	assessments					789:799	behavioral assessments	778:799	behavioral assessments	778:799	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	6	10	theme	behavioral	778:787	arg1	field					817:821	open field	812:821	open field	812:821	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	6	10	theme	behavioral	778:787	arg1	tests					864:868	forced swimming tests	848:868	forced swimming tests	848:868	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	6	10	theme	behavioral	778:787	arg1	preference					832:841	sucrose preference	824:841	sucrose preference	824:841	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	7	11	theme	reactive	911:918	arg1	species					927:933	reactive oxygen species	911:933	reactive oxygen species	911:933	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	7	12	theme	lactic	1057:1062	arg1	LAB					1079:1081	LAB	1079:1081	LAB	1079:1081	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	7	12	theme	lactic	1057:1062	arg1	bacteria					1069:1076	their fecal lactic acid bacteria	1045:1076	their fecal lactic acid bacteria (LAB) content	1045:1090	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	8	13	theme	increased	1210:1218	arg1	time					1231:1234	increased immobility time	1210:1234	increased immobility time	1210:1234	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	11	14	from	hippocampus	1758:1768	arg1	modulation					1675:1684	modulation	1675:1684	modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota	1675:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	14	from	hippocampus	1758:1768	arg1	content					1796:1802	the LAB content	1788:1802	the LAB content of the gut microbiota	1788:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	7	15	theme	hippocampal	987:997	arg1	tissues					999:1005	the hippocampal tissues	983:1005	the hippocampal tissues of these animals	983:1022	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	11	16	from	alteration	1774:1783	arg1	modulation					1675:1684	modulation	1675:1684	modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota	1675:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	16	from	alteration	1774:1783	arg1	content					1796:1802	the LAB content	1788:1802	the LAB content of the gut microbiota	1788:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	5	17	theme	1 ×	711:713	arg1	CFU/mouse					719:727	1 × 109 CFU/mouse	711:727	1 × 109 CFU/mouse	711:727	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	5	17	theme	1 ×	711:713	arg1	week					705:708	one week	701:708	one week (1 × 109 CFU/mouse)	701:728	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	9	18	theme	LL95	1261:1264	arg1	treatment					1266:1274	LL95 treatment	1261:1274	LL95 treatment	1261:1274	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	10	19	from	content	1485:1491	arg1	animals					1502:1508	these animals	1496:1508	these animals	1496:1508	Furthermore, LL95 intervention increased the fecal LAB content in these animals, suggesting changes in the gut microbiota.
35306095	2	20	theme	microbiota	243:252	arg1	composition					213:223	the composition	209:223	the composition of the intestinal microbiota	209:252	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	2	20	theme	microbiota	243:252	arg1	neuroinflammation					153:169	neuroinflammation	153:169	neuroinflammation	153:169	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	2	20	theme	microbiota	243:252	arg1	activation					172:181	activation	172:181	activation of the immune system	172:202	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	9	21	theme	LPS-induced	1289:1299	arg1	increase					1301:1308	the LPS-induced increase	1285:1308	the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent	1285:1427	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	7	22	theme	factor-α	951:958	arg1	levels					901:906	the levels	897:906	the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals	897:1022	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	8	23	from	behaviors	1148:1156	arg1	mice					1161:1164	mice	1161:1164	mice	1161:1164	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	5	24	theme	depression-like	631:645	arg1	symptoms					647:654	depression-like symptoms	631:654	depression-like symptoms	631:654	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	10	25	from	changes	1522:1528	arg1	microbiota					1541:1550	the gut microbiota	1533:1550	the gut microbiota	1533:1550	Furthermore, LL95 intervention increased the fecal LAB content in these animals, suggesting changes in the gut microbiota.
35306095	3	26	theme	probiotic	343:351	arg1	intervention					353:364	a probiotic intervention	341:364	a probiotic intervention	341:364	This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp.
35306095	4	27	theme	depression-like	481:495	arg1	model					503:507	a lipopolysaccharide (LPS)-induced depression-like mouse model	446:507	a lipopolysaccharide (LPS)-induced depression-like mouse model	446:507	cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model.
35306095	10	28	theme	LAB	1481:1483	arg1	content					1485:1491	the fecal LAB content	1471:1491	the fecal LAB content in these animals	1471:1508	Furthermore, LL95 intervention increased the fecal LAB content in these animals, suggesting changes in the gut microbiota.
35306095	7	29	theme	species	927:933	arg1	levels					901:906	the levels	897:906	the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals	897:1022	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	0	30	theme	Beneficial	0:9	arg1	effects					11:17	Beneficial effects	0:17	Beneficial effects of Lactococcus lactis	0:39	Beneficial effects of Lactococcus lactis subsp.
35306095	9	31	theme	reactive	1335:1342	arg1	species					1351:1357	reactive oxygen species	1335:1357	reactive oxygen species	1335:1357	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	5	32	theme	intraperitoneal	595:609	arg1	injection					611:619	intraperitoneal injection	595:619	intraperitoneal injection to induce depression-like symptoms	595:654	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	1	33	theme	depression-like	90:104	arg1	model					106:110	an LPS-induced depression-like model	75:110	an LPS-induced depression-like model in mice	75:118	cremoris LL95 treatment in an LPS-induced depression-like model in mice.
35306095	3	34	theme	lactis	384:389	arg1	subsp					391:395	Lactococcus lactis subsp	372:395	Lactococcus lactis subsp	372:395	This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp.
35306095	7	35	from	levels	901:906	arg1	tissues					999:1005	the hippocampal tissues	983:1005	the hippocampal tissues of these animals	983:1022	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	9	36	theme	species	1351:1357	arg1	levels					1325:1330	hippocampal levels	1313:1330	hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent	1313:1427	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	11	37	theme	pro-inflammatory	1714:1729	arg1	cytokine					1731:1738	pro-inflammatory cytokine	1714:1738	pro-inflammatory cytokine expression	1714:1749	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	0	38	theme	lactis	34:39	arg1	effects					11:17	Beneficial effects	0:17	Beneficial effects of Lactococcus lactis	0:39	Beneficial effects of Lactococcus lactis subsp.
35306095	9	39	theme	tumor	1363:1367	arg1	factor-α					1378:1385	tumor necrosis factor-α	1363:1385	tumor necrosis factor-α	1363:1385	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	6	40	theme	swimming	855:862	arg1	tests					864:868	forced swimming tests	848:868	forced swimming tests	848:868	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	11	41	theme	status	1703:1708	arg1	modulation					1675:1684	modulation	1675:1684	modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota	1675:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	42	theme	expression	1740:1749	arg1	modulation					1675:1684	modulation	1675:1684	modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota	1675:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	9	43	theme	interleukin-1β	1395:1408	arg1	levels					1325:1330	hippocampal levels	1313:1330	hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent	1313:1427	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	10	44	theme	gut	1537:1539	arg1	microbiota					1541:1550	the gut microbiota	1533:1550	the gut microbiota	1533:1550	Furthermore, LL95 intervention increased the fecal LAB content in these animals, suggesting changes in the gut microbiota.
35306095	2	45	theme	Clinical	121:128	arg1	evidence					130:137	Clinical evidence	121:137	Clinical evidence	121:137	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	11	46	theme	LPS-induced	1624:1634	arg1	depression					1636:1645	LPS-induced depression	1624:1645	LPS-induced depression	1624:1645	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	8	47	theme	LPS-induced	1120:1130	arg1	behaviors					1148:1156	LPS-induced depression-like behaviors	1120:1156	LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test	1120:1258	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	5	48	theme	LL95	692:695	arg1	administration					674:687	oral administration	669:687	oral administration of LL95 for one week (1 × 109 CFU/mouse)	669:728	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	2	49	theme	depression	287:296	arg1	pathology					274:282	the pathology	270:282	the pathology of depression	270:296	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	1	50	from	treatment	62:70	arg1	model					106:110	an LPS-induced depression-like model	75:110	an LPS-induced depression-like model in mice	75:118	cremoris LL95 treatment in an LPS-induced depression-like model in mice.
35306095	4	51	theme	mood	428:431	arg1	disorders					433:441	mood disorders	428:441	mood disorders	428:441	cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model.
35306095	7	52	theme	acid	1064:1067	arg1	LAB					1079:1081	LAB	1079:1081	LAB	1079:1081	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	7	52	theme	acid	1064:1067	arg1	bacteria					1069:1076	their fecal lactic acid bacteria	1045:1076	their fecal lactic acid bacteria (LAB) content	1045:1090	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	6	53	theme	assessments	789:799	arg1	series					768:773	a series	766:773	a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests	766:868	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	7	54	theme	bacteria	1069:1076	arg1	content					1084:1090	their fecal lactic acid bacteria (LAB) content	1045:1090	their fecal lactic acid bacteria (LAB) content	1045:1090	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	10	55	theme	LL95	1443:1446	arg1	intervention					1448:1459	LL95 intervention	1443:1459	LL95 intervention	1443:1459	Furthermore, LL95 intervention increased the fecal LAB content in these animals, suggesting changes in the gut microbiota.
35306095	7	56	theme	fecal	1051:1055	arg1	LAB					1079:1081	LAB	1079:1081	LAB	1079:1081	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	7	56	theme	fecal	1051:1055	arg1	bacteria					1069:1076	their fecal lactic acid bacteria	1045:1076	their fecal lactic acid bacteria (LAB) content	1045:1090	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	8	57	theme	sucrose	1187:1193	arg1	preference					1195:1204	decreased sucrose preference	1177:1204	decreased sucrose preference	1177:1204	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	11	58	theme	gut	1811:1813	arg1	microbiota					1815:1824	the gut microbiota	1807:1824	the gut microbiota	1807:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	2	59	theme	intestinal	232:241	arg1	microbiota					243:252	the intestinal microbiota	228:252	the intestinal microbiota	228:252	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	8	60	theme	forced	1243:1248	arg1	test					1255:1258	the forced swim test	1239:1258	the forced swim test	1239:1258	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	9	61	from	increase	1301:1308	arg1	levels					1325:1330	hippocampal levels	1313:1330	hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent	1313:1427	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	4	62	theme	-induced	472:479	arg1	model					503:507	a lipopolysaccharide (LPS)-induced depression-like mouse model	446:507	a lipopolysaccharide (LPS)-induced depression-like mouse model	446:507	cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model.
35306095	8	63	theme	immobility	1220:1229	arg1	time					1231:1234	increased immobility time	1210:1234	increased immobility time	1210:1234	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	7	64	theme	animals	1016:1022	arg1	tissues					999:1005	the hippocampal tissues	983:1005	the hippocampal tissues of these animals	983:1022	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	5	65	theme	5 mg/kg	579:585	arg1	LPS					587:589	5 mg/kg LPS	579:589	5 mg/kg LPS	579:589	C57BL/6 mice were randomly divided into four groups and treated with 5 mg/kg LPS via intraperitoneal injection to induce depression-like symptoms, followed by oral administration of LL95 for one week (1 × 109 CFU/mouse).
35306095	11	66	from	expression	1740:1749	arg1	alteration					1774:1783	alteration	1774:1783	alteration in the LAB content of the gut microbiota	1774:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	66	from	expression	1740:1749	arg1	hippocampus					1758:1768	the hippocampus	1754:1768	the hippocampus	1754:1768	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	67	from	status	1703:1708	arg1	alteration					1774:1783	alteration	1774:1783	alteration in the LAB content of the gut microbiota	1774:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	67	from	status	1703:1708	arg1	hippocampus					1758:1768	the hippocampus	1754:1768	the hippocampus	1754:1768	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	7	68	theme	interleukin-1β	965:978	arg1	levels					901:906	the levels	897:906	the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals	897:1022	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	8	69	dep	mice	1161:1164	arg1	including					1167:1175	including	1167:1175	including decreased sucrose preference and increased immobility time in the forced swim test	1167:1258	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	7	70	theme	tumor	936:940	arg1	factor-α					951:958	tumor necrosis factor-α	936:958	tumor necrosis factor-α	936:958	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	3	71	theme	Lactococcus	372:382	arg1	lactis					384:389	Lactococcus lactis	372:389	Lactococcus lactis subsp	372:395	This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp.
35306095	4	72	theme	mouse	497:501	arg1	model					503:507	a lipopolysaccharide (LPS)-induced depression-like mouse model	446:507	a lipopolysaccharide (LPS)-induced depression-like mouse model	446:507	cremoris LL95 in ameliorating mood disorders in a lipopolysaccharide (LPS)-induced depression-like mouse model.
35306095	11	73	theme	LAB	1792:1794	arg1	content					1796:1802	the LAB content	1788:1802	the LAB content of the gut microbiota	1788:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	9	74	theme	hippocampal	1313:1323	arg1	levels					1325:1330	hippocampal levels	1313:1330	hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent	1313:1427	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	7	75	theme	necrosis	942:949	arg1	factor-α					951:958	tumor necrosis factor-α	936:958	tumor necrosis factor-α	936:958	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	3	76	theme	intervention	353:364	arg1	effectiveness					324:336	the effectiveness	320:336	the effectiveness of a probiotic intervention	320:364	This study evaluated the effectiveness of a probiotic intervention using Lactococcus lactis subsp.
35306095	10	77	theme	fecal	1475:1479	arg1	content					1485:1491	the fecal LAB content	1471:1491	the fecal LAB content in these animals	1471:1508	Furthermore, LL95 intervention increased the fecal LAB content in these animals, suggesting changes in the gut microbiota.
35306095	1	78	theme	LPS-induced	78:88	arg1	model					106:110	an LPS-induced depression-like model	75:110	an LPS-induced depression-like model in mice	75:118	cremoris LL95 treatment in an LPS-induced depression-like model in mice.
35306095	11	79	from	modulation	1675:1684	arg1	alteration					1774:1783	alteration	1774:1783	alteration in the LAB content of the gut microbiota	1774:1824	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	79	from	modulation	1675:1684	arg1	hippocampus					1758:1768	the hippocampus	1754:1768	the hippocampus	1754:1768	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	2	80	theme	system	197:202	arg1	composition					213:223	the composition	209:223	the composition of the intestinal microbiota	209:252	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	2	80	theme	system	197:202	arg1	neuroinflammation					153:169	neuroinflammation	153:169	neuroinflammation	153:169	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	2	80	theme	system	197:202	arg1	activation					172:181	activation	172:181	activation of the immune system	172:202	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	0	81	theme	Lactococcus	22:32	arg1	lactis					34:39	Lactococcus lactis	22:39	Lactococcus lactis	22:39	Beneficial effects of Lactococcus lactis subsp.
35306095	9	82	theme	oxygen	1344:1349	arg1	species					1351:1357	reactive oxygen species	1335:1357	reactive oxygen species	1335:1357	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	7	83	theme	oxygen	920:925	arg1	species					927:933	reactive oxygen species	911:933	reactive oxygen species	911:933	In addition, we evaluated the levels of reactive oxygen species, tumor necrosis factor-α, and interleukin-1β in the hippocampal tissues of these animals, and also determined their fecal lactic acid bacteria (LAB) content.
35306095	2	84	theme	immune	190:195	arg1	system					197:202	the immune system	186:202	the immune system	186:202	Clinical evidence suggests that neuroinflammation, activation of the immune system, and the composition of the intestinal microbiota are involved in the pathology of depression.
35306095	11	85	theme	oxidative	1693:1701	arg1	status					1703:1708	oxidative status	1693:1708	oxidative status	1693:1708	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	86	theme	antidepressant-like	1593:1611	arg1	effects					1613:1619	antidepressant-like effects	1593:1619	antidepressant-like effects	1593:1619	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	9	87	theme	necrosis	1369:1376	arg1	factor-α					1378:1385	tumor necrosis factor-α	1363:1385	tumor necrosis factor-α	1363:1385	LL95 treatment reversed the LPS-induced increase in hippocampal levels of reactive oxygen species and tumor necrosis factor-α, and of interleukin-1β to a lesser extent.
35306095	6	88	theme	forced	848:853	arg1	tests					864:868	forced swimming tests	848:868	forced swimming tests	848:868	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35306095	1	89	from	model	106:110	arg1	mice					115:118	mice	115:118	mice	115:118	cremoris LL95 treatment in an LPS-induced depression-like model in mice.
35306095	8	90	theme	LL95	1093:1096	arg1	intervention					1098:1109	LL95 intervention	1093:1109	LL95 intervention	1093:1109	LL95 intervention improved LPS-induced depression-like behaviors in mice, including decreased sucrose preference and increased immobility time in the forced swim test.
35306095	11	91	theme	cytokine	1731:1738	arg1	expression					1740:1749	pro-inflammatory cytokine expression	1714:1749	pro-inflammatory cytokine expression	1714:1749	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	11	92	dep	status	1703:1708	arg1	the					1689:1691	the	1689:1691	the	1689:1691	These findings suggest that LL95 exerts antidepressant-like effects in LPS-induced depression, which may be attributed to modulation of the oxidative status and pro-inflammatory cytokine expression in the hippocampus and alteration in the LAB content of the gut microbiota.
35306095	6	93	theme	sucrose	824:830	arg1	preference					832:841	sucrose preference	824:841	sucrose preference	824:841	The animals were then subjected to a series of behavioral assessments, including open field, sucrose preference, and forced swimming tests.
35264695	2	0	from	information	157:167	arg1	activity					266:273	the potential antidiabetic activity	239:273	the potential antidiabetic activity of its major active compounds	239:303	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	2	0	from	information	157:167	arg1	composition					189:199	the polyphenolic composition	172:199	the polyphenolic composition of Kluai Hin banana peel and pulp	172:233	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	9	1	theme	α-glucosidase	1472:1484	arg1	activity					1486:1493	α-glucosidase activity	1472:1493	α-glucosidase activity	1472:1493	Peel extracts inhibited α-glucosidase activity, whereas pulp extracts had no effect.
35264695	3	2	theme	W	446:446	arg1	water					437:441	water	437:441	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	11	3	theme	commercial	1923:1932	arg1	drug					1934:1937	a commercial drug	1921:1937	a commercial drug	1921:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	2	4	theme	antidiabetic	253:264	arg1	activity					266:273	the potential antidiabetic activity	239:273	the potential antidiabetic activity of its major active compounds	239:303	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	9	5	theme	pulp	1504:1507	arg1	extracts					1509:1516	pulp extracts	1504:1516	pulp extracts	1504:1516	Peel extracts inhibited α-glucosidase activity, whereas pulp extracts had no effect.
35264695	11	6	theme	postprandial	1826:1837	arg1	response					1849:1856	postprandial glycaemic response	1826:1856	postprandial glycaemic response	1826:1856	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	2	7	theme	major	282:286	arg1	compounds					295:303	its major active compounds	278:303	its major active compounds	278:303	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	4	8	dep	banana	692:697	arg1	pulp					708:711	pulp	708:711	pulp	708:711	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	4	8	dep	banana	692:697	arg1	peel					699:702	peel	699:702	peel	699:702	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	6	9	theme	unidentified	988:999	arg1	peaks					1012:1016	unidentified polyphenol peaks	988:1016	unidentified polyphenol peaks of Kluai Hin	988:1029	Furthermore, unidentified polyphenol peaks of Kluai Hin were further explored by HPLC-ESI-QTOF-MS.
35264695	5	10	dep	-Gallocatechin	841:854	arg1	-					839:839	-	839:839	-	839:839	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	1	11	theme	functional	53:62	arg1	polyphenols					64:74	functional polyphenols	53:74	functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function	53:145	peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function.
35264695	10	12	theme	α-amylase	1593:1601	arg1	activity					1603:1610	α-amylase activity	1593:1610	α-amylase activity	1593:1610	In addition, all standards, except gallocatechin, activated α-amylase activity, while, gallocatechin inhibited α-glucosidase activity better than acarbose.
35264695	8	13	theme	fresh	1237:1241	arg1	peel					1260:1263	the fresh and freeze-dried peel	1233:1263	the fresh and freeze-dried peel of	1233:1266	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	2	14	dep	banana	214:219	arg1	pulp					230:233	pulp	230:233	pulp	230:233	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	2	14	dep	banana	214:219	arg1	peel					221:224	peel	221:224	peel	221:224	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	2	15	theme	Hin	210:212	arg1	banana					214:219	Kluai Hin banana peel and pulp	204:233	Kluai Hin banana peel and pulp	204:233	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	5	16	located	detected	897:904	arg2	polyphenol					878:887	the most abundant polyphenol	860:887	the most abundant polyphenol	860:887	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	5	16	located	detected	897:904	arg1	extracts					950:957	all fresh and freeze-dried pulp and peel extracts	909:957	all fresh and freeze-dried pulp and peel extracts	909:957	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	5	16	located	detected	897:904	arg2	-Gallocatechin					841:854	(-)-Gallocatechin	838:854	(-)-Gallocatechin	838:854	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	8	17	theme	freeze-dried	1247:1258	arg1	peel					1260:1263	the fresh and freeze-dried peel	1233:1263	the fresh and freeze-dried peel of	1233:1266	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	7	18	theme	565.03 mg	1191:1199	arg1	peel					1185:1188	the freeze-dried peel	1168:1188	the freeze-dried peel (565.03 mg GAE/100 g)	1168:1210	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	7	18	theme	565.03 mg	1191:1199	arg1	GAE/100 g					1201:1209	565.03 mg GAE/100 g	1191:1209	565.03 mg GAE/100 g	1191:1209	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	4	19	theme	activities	629:638	arg1	inhibition					587:596	inhibition	587:596	inhibition of α-amylase and α-glucosidase activities	587:638	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	11	20	theme	glycaemic	1839:1847	arg1	response					1849:1856	postprandial glycaemic response	1826:1856	postprandial glycaemic response	1826:1856	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	8	21	theme	IC50	1278:1281	arg1	values					1283:1288	IC50 values	1278:1288	IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml	1278:1351	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	1	22	theme	antidiabetic	125:136	arg1	function					138:145	its potential antidiabetic function	111:145	its potential antidiabetic function	111:145	peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function.
35264695	11	23	theme	diabetes	1868:1875	arg1	risk					1877:1880	diabetes risk	1868:1880	diabetes risk	1868:1880	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	8	24	dep	A	1213:1213	arg1	W					1215:1215	W	1215:1215	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).	1213:1446	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	8	25	theme	activity	1304:1311	arg1	2.66 ± 0.07 mg/ml					1313:1329	α-amylase activity 2.66 ± 0.07 mg/ml	1294:1329	α-amylase activity 2.66 ± 0.07 mg/ml	1294:1329	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	5	26	theme	pulp	936:939	arg1	extracts					950:957	all fresh and freeze-dried pulp and peel extracts	909:957	all fresh and freeze-dried pulp and peel extracts	909:957	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	11	27	theme	response	1849:1856	arg1	management					1812:1821	the management	1808:1821	the management of postprandial glycaemic response to reduce diabetes risk	1808:1880	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	4	28	theme	antidiabetic	810:821	arg1	acarbose					828:835	the antidiabetic drug acarbose	806:835	the antidiabetic drug acarbose	806:835	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	11	29	theme	peel	1768:1771	arg1	use					1744:1746	the use	1740:1746	the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug	1740:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	4	30	theme	Hin	688:690	arg1	banana					692:697	Kluai Hin banana peel and pulp	682:711	Kluai Hin banana peel and pulp	682:711	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	3	31	theme	banana	406:411	arg1	peel					413:416	fresh and freeze-dried Kluai Hin banana peel	373:416	fresh and freeze-dried Kluai Hin banana peel	373:416	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	5	32	theme	peel	945:948	arg1	extracts					950:957	all fresh and freeze-dried pulp and peel extracts	909:957	all fresh and freeze-dried pulp and peel extracts	909:957	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	7	33	dep	more	1113:1116	arg1	content					1133:1139	total phenolic content	1118:1139	total phenolic content (811.56 mg GAE/100 g)	1118:1161	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	7	33	dep	more	1113:1116	arg1	GAE/100 g					1152:1160	811.56 mg GAE/100 g	1142:1160	811.56 mg GAE/100 g	1142:1160	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	11	34	with	diabetes	1907:1914	arg1	drug					1934:1937	a commercial drug	1921:1937	a commercial drug	1921:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	4	35	dep	polyphenols	742:752	arg1	acid					796:799	gallic acid	789:799	gallic acid	789:799	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	4	35	dep	polyphenols	742:752	arg1	-catechin					758:766	-catechin	758:766	-catechin	758:766	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	4	36	theme	major	736:740	arg1	polyphenols					742:752	its major polyphenols	732:752	its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid)	732:800	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	4	37	theme	crude	662:666	arg1	extracts					668:675	crude extracts	662:675	crude extracts from Kluai Hin banana peel and pulp	662:711	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	11	38	theme	banana	1761:1766	arg1	peel					1768:1771	Kluai Hin banana peel	1751:1771	Kluai Hin banana peel	1751:1771	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	3	39	theme	flavonoids	459:468	arg1	water					437:441	water	437:441	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	11	40	theme	Kluai	1751:1755	arg1	peel					1768:1771	Kluai Hin banana peel	1751:1771	Kluai Hin banana peel	1751:1771	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	7	41	theme	phenolic	1124:1131	arg1	content					1133:1139	total phenolic content	1118:1139	total phenolic content (811.56 mg GAE/100 g)	1118:1161	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	7	41	theme	phenolic	1124:1131	arg1	GAE/100 g					1152:1160	811.56 mg GAE/100 g	1142:1160	811.56 mg GAE/100 g	1142:1160	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	3	42	dep	peel	413:416	arg1	methanol					428:435	methanol	428:435	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	2	43	theme	polyphenolic	176:187	arg1	composition					189:199	the polyphenolic composition	172:199	the polyphenolic composition of Kluai Hin banana peel and pulp	172:233	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	11	44	theme	potential	1778:1786	arg1	food					1799:1802	a potential functional food	1776:1802	a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug	1776:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	9	45	theme	Peel	1448:1451	arg1	extracts					1453:1460	Peel extracts	1448:1460	Peel extracts	1448:1460	Peel extracts inhibited α-glucosidase activity, whereas pulp extracts had no effect.
35264695	11	46	theme	Hin	1757:1759	arg1	peel					1768:1771	Kluai Hin banana peel	1751:1771	Kluai Hin banana peel	1751:1771	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	11	47	theme	further	1713:1719	arg1	investigation					1721:1733	a further investigation	1711:1733	a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug	1711:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	3	48	dep	methanol	428:435	arg1	A					505:505	A	505:505	A	505:505	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	48	dep	methanol	428:435	arg1	W					503:503	W	503:503	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	48	dep	methanol	428:435	arg1	water					437:441	water	437:441	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	48	dep	methanol	428:435	arg1	acids					529:533	50:49:1 for phenolic acids	508:533	50:49:1 for phenolic acids	508:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	7	49	theme	fresh	1084:1088	arg1	extract					1095:1101	A fresh peel extract	1082:1101	A fresh peel extract	1082:1101	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	5	50	theme	abundant	869:876	arg1	-Gallocatechin					841:854	(-)-Gallocatechin	838:854	(-)-Gallocatechin	838:854	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	5	50	theme	abundant	869:876	arg1	polyphenol					878:887	the most abundant polyphenol	860:887	the most abundant polyphenol	860:887	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	3	51	theme	acetone	474:480	arg1	water					437:441	water	437:441	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	2	52	theme	active	288:293	arg1	compounds					295:303	its major active compounds	278:303	its major active compounds	278:303	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	6	53	theme	Hin	1027:1029	arg1	peaks					1012:1016	unidentified polyphenol peaks	988:1016	unidentified polyphenol peaks of Kluai Hin	988:1029	Furthermore, unidentified polyphenol peaks of Kluai Hin were further explored by HPLC-ESI-QTOF-MS.
35264695	11	54	from	food	1799:1802	arg1	management					1893:1902	the management	1889:1902	the management of diabetes with a commercial drug	1889:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	2	55	theme	potential	243:251	arg1	activity					266:273	the potential antidiabetic activity	239:273	the potential antidiabetic activity of its major active compounds	239:303	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	3	56	theme	acetic	488:493	arg1	acid					495:498	acetic acid	488:498	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	6	57	theme	polyphenol	1001:1010	arg1	peaks					1012:1016	unidentified polyphenol peaks	988:1016	unidentified polyphenol peaks of Kluai Hin	988:1029	Furthermore, unidentified polyphenol peaks of Kluai Hin were further explored by HPLC-ESI-QTOF-MS.
35264695	3	58	theme	water	482:486	arg1	water					437:441	water	437:441	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	59	theme	acid	495:498	arg1	water					437:441	water	437:441	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	5	60	theme	fresh	913:917	arg1	extracts					950:957	all fresh and freeze-dried pulp and peel extracts	909:957	all fresh and freeze-dried pulp and peel extracts	909:957	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	10	61	theme	α-glucosidase	1644:1656	arg1	activity					1658:1665	α-glucosidase activity	1644:1665	α-glucosidase activity	1644:1665	In addition, all standards, except gallocatechin, activated α-amylase activity, while, gallocatechin inhibited α-glucosidase activity better than acarbose.
35264695	4	62	theme	gallic	789:794	arg1	acid					796:799	gallic acid	789:799	gallic acid	789:799	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	8	63	theme	peel	1260:1263	arg1	extraction					1219:1228	A extraction	1217:1228	A extraction of the fresh and freeze-dried peel of	1217:1266	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	5	64	theme	freeze-dried	923:934	arg1	extracts					950:957	all fresh and freeze-dried pulp and peel extracts	909:957	all fresh and freeze-dried pulp and peel extracts	909:957	(-)-Gallocatechin was the most abundant polyphenol and was detected in all fresh and freeze-dried pulp and peel extracts by RP-HPLC-DAD.
35264695	2	65	theme	Kluai	204:208	arg1	banana					214:219	Kluai Hin banana peel and pulp	204:233	Kluai Hin banana peel and pulp	204:233	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	4	66	theme	α-amylase	601:609	arg1	activities					629:638	α-amylase and α-glucosidase activities	601:638	α-amylase and α-glucosidase activities	601:638	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	11	67	theme	diabetes	1907:1914	arg1	management					1893:1902	the management	1889:1902	the management of diabetes with a commercial drug	1889:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	4	68	theme	α-glucosidase	615:627	arg1	activities					629:638	α-amylase and α-glucosidase activities	601:638	α-amylase and α-glucosidase activities	601:638	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	7	69	theme	A	1078:1078	arg1	W					1080:1080	The A:W	1074:1080	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).	1074:1211	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	1	70	theme	potential	115:123	arg1	function					138:145	its potential antidiabetic function	111:145	its potential antidiabetic function	111:145	peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function.
35264695	4	71	theme	drug	823:826	arg1	acarbose					828:835	the antidiabetic drug acarbose	806:835	the antidiabetic drug acarbose	806:835	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	8	72	theme	α-amylase	1294:1302	arg1	2.66 ± 0.07 mg/ml					1313:1329	α-amylase activity 2.66 ± 0.07 mg/ml	1294:1329	α-amylase activity 2.66 ± 0.07 mg/ml	1294:1329	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	7	73	theme	freeze-dried	1172:1183	arg1	peel					1185:1188	the freeze-dried peel	1168:1188	the freeze-dried peel (565.03 mg GAE/100 g)	1168:1210	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	7	73	theme	freeze-dried	1172:1183	arg1	GAE/100 g					1201:1209	565.03 mg GAE/100 g	1191:1209	565.03 mg GAE/100 g	1191:1209	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	4	74	from	banana	692:697	arg1	extracts					668:675	crude extracts	662:675	crude extracts from Kluai Hin banana peel and pulp	662:711	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	3	75	from	polyphenols	346:356	arg1	extracts					361:368	extracts	361:368	extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS	361:570	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	4	76	theme	Kluai	682:686	arg1	banana					692:697	Kluai Hin banana peel and pulp	682:711	Kluai Hin banana peel and pulp	682:711	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	3	77	theme	peel	413:416	arg1	extracts					361:368	extracts	361:368	extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS	361:570	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	7	78	dep	W	1080:1080	arg1	contained					1103:1111	contained	1103:1111	contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g)	1103:1210	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	7	79	theme	811.56 mg	1142:1150	arg1	content					1133:1139	total phenolic content	1118:1139	total phenolic content (811.56 mg GAE/100 g)	1118:1161	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	7	79	theme	811.56 mg	1142:1150	arg1	GAE/100 g					1152:1160	811.56 mg GAE/100 g	1142:1160	811.56 mg GAE/100 g	1142:1160	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	3	80	theme	Hin	402:404	arg1	peel					413:416	fresh and freeze-dried Kluai Hin banana peel	373:416	fresh and freeze-dried Kluai Hin banana peel	373:416	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	1	81	theme	polyphenols	64:74	arg1	source					43:48	a source	41:48	a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function	41:145	peel as a source of functional polyphenols identified by HPLC-ESI-QTOF-MS and its potential antidiabetic function.
35264695	2	82	theme	banana	214:219	arg1	activity					266:273	the potential antidiabetic activity	239:273	the potential antidiabetic activity of its major active compounds	239:303	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	2	82	theme	banana	214:219	arg1	composition					189:199	the polyphenolic composition	172:199	the polyphenolic composition of Kluai Hin banana peel and pulp	172:233	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	8	83	theme	inhibitory	1376:1385	arg1	activity					1387:1394	its inhibitory activity	1372:1394	its inhibitory activity	1372:1394	A:W:A extraction of the fresh and freeze-dried peel of exhibited IC50 values for α-amylase activity 2.66 ± 0.07 mg/ml and 2.97 ± 0.00 mg/ml, respectively, but its inhibitory activity was lower than acarbose (IC50 = 0.25 ± 0.01 mg/ml).
35264695	9	84	contain	had	1518:1520	arg1	extracts					1509:1516	pulp extracts	1504:1516	pulp extracts	1504:1516	Peel extracts inhibited α-glucosidase activity, whereas pulp extracts had no effect.
35264695	9	84	contain	had	1518:1520	arg2	effect					1525:1530	no effect	1522:1530	no effect	1522:1530	Peel extracts inhibited α-glucosidase activity, whereas pulp extracts had no effect.
35264695	4	85	dep	-catechin	758:766	arg1	-epicatechin					772:783	-epicatechin	772:783	-epicatechin	772:783	Additionally, inhibition of α-amylase and α-glucosidase activities was investigated with crude extracts from Kluai Hin banana peel and pulp, and compared with its major polyphenols ((+)-catechin, (-)-epicatechin and gallic acid) and the antidiabetic drug acarbose.
35264695	7	86	theme	total	1118:1122	arg1	content					1133:1139	total phenolic content	1118:1139	total phenolic content (811.56 mg GAE/100 g)	1118:1161	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	7	86	theme	total	1118:1122	arg1	GAE/100 g					1152:1160	811.56 mg GAE/100 g	1142:1160	811.56 mg GAE/100 g	1142:1160	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	3	87	theme	freeze-dried	383:394	arg1	peel					413:416	fresh and freeze-dried Kluai Hin banana peel	373:416	fresh and freeze-dried Kluai Hin banana peel	373:416	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	88	theme	phenolic	520:527	arg1	A					505:505	A	505:505	A	505:505	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	88	theme	phenolic	520:527	arg1	acids					529:533	50:49:1 for phenolic acids	508:533	50:49:1 for phenolic acids	508:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	89	theme	pulp	422:425	arg1	extracts					361:368	extracts	361:368	extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS	361:570	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	0	90	dep	Linn	26:29	arg1	Musa					11:14	Musa	11:14	Musa	11:14	Kluai Hin (Musa sapientum Linn.)
35264695	3	91	theme	fresh	373:377	arg1	peel					413:416	fresh and freeze-dried Kluai Hin banana peel	373:416	fresh and freeze-dried Kluai Hin banana peel	373:416	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	3	92	theme	M	444:444	arg1	water					437:441	water	437:441	methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids	428:533	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
35264695	11	93	theme	functional	1788:1797	arg1	food					1799:1802	a potential functional food	1776:1802	a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug	1776:1937	Therefore, we propose a further investigation into the use of Kluai Hin banana peel as a potential functional food for the management of postprandial glycaemic response to reduce diabetes risk and in the management of diabetes with a commercial drug.
35264695	7	94	theme	peel	1090:1093	arg1	extract					1095:1101	A fresh peel extract	1082:1101	A fresh peel extract	1082:1101	The A:W:A fresh peel extract contained more total phenolic content (811.56 mg GAE/100 g) than the freeze-dried peel (565.03 mg GAE/100 g).
35264695	2	95	theme	compounds	295:303	arg1	activity					266:273	the potential antidiabetic activity	239:273	the potential antidiabetic activity of its major active compounds	239:303	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	2	95	theme	compounds	295:303	arg1	composition					189:199	the polyphenolic composition	172:199	the polyphenolic composition of Kluai Hin banana peel and pulp	172:233	To date, information on the polyphenolic composition of Kluai Hin banana peel and pulp and the potential antidiabetic activity of its major active compounds is limited.
35264695	0	96	dep	Hin	6:8	arg1	Linn					26:29	Linn	26:29	Linn	26:29	Kluai Hin (Musa sapientum Linn.)
35264695	3	97	theme	80:20 for	449:457	arg1	flavonoids					459:468	80:20 for flavonoids	449:468	80:20 for flavonoids	449:468	This study aimed to identify polyphenols in extracts of fresh and freeze-dried Kluai Hin banana peel and pulp (methanol:water; M:W, 80:20 for flavonoids and acetone:water:acetic acid; A:W:A, 50:49:1 for phenolic acids) by RP-HPLC-DAD and HPLC-ESI-QTOF-MS.
37255290	9	0	theme	immunity	1486:1493	arg1	capacity					1428:1435	the capacity	1424:1435	the capacity of the intestinal barrier and intestinal mucosal immunity	1424:1493	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	6	1	theme	MUC2	931:934	arg1	mRNA					937:940	mucin (MUC2) mRNA	924:940	mucin (MUC2) mRNA	924:940	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	3	2	theme	Bifidobacterium	307:321	arg1	BB12					340:343	BB12	340:343	BB12	340:343	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	2	theme	Bifidobacterium	307:321	arg1	BLBB					350:353	BLBB	350:353	BLBB	350:353	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	2	theme	Bifidobacterium	307:321	arg1	BL					336:337	BL	336:337	BL	336:337	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	2	theme	Bifidobacterium	307:321	arg1	BB					332:333	BB	332:333	BB	332:333	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	2	theme	Bifidobacterium	307:321	arg1	strains					323:329	Bifidobacterium strains	307:329	Bifidobacterium strains (BB, BL, BB12, and BLBB)	307:354	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	6	3	theme	inflammatory	1016:1027	arg1	cytokines					1029:1037	inflammatory cytokines	1016:1037	inflammatory cytokines	1016:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	4	theme	levels	1006:1011	arg1	IL-1β					1040:1044	IL-1β	1040:1044	IL-1β	1040:1044	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	4	theme	levels	1006:1011	arg1	level					844:848	an increased expression level	820:848	an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines	820:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	4	theme	levels	1006:1011	arg1	IL-6					1047:1050	IL-6	1047:1050	IL-6	1047:1050	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	4	theme	levels	1006:1011	arg1	TNF-α					1057:1061	TNF-α	1057:1061	TNF-α	1057:1061	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	4	5	theme	IgA+	579:582	arg1	plasma					584:589	IgA+ plasma	579:589	IgA+ plasma	579:589	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	3	6	dep	strains	323:329	arg1	BB12					340:343	BB12	340:343	BB12	340:343	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	6	dep	strains	323:329	arg1	BLBB					350:353	BLBB	350:353	BLBB	350:353	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	6	dep	strains	323:329	arg1	BL					336:337	BL	336:337	BL	336:337	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	6	dep	strains	323:329	arg1	BB					332:333	BB	332:333	BB	332:333	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	3	6	dep	strains	323:329	arg1	strains					323:329	Bifidobacterium strains	307:329	Bifidobacterium strains (BB, BL, BB12, and BLBB)	307:354	The protection by Bifidobacterium strains (BB, BL, BB12, and BLBB) was evaluated in mice injected with lipopolysaccharide (LPS).
37255290	6	7	theme	decreased	985:993	arg1	levels					1006:1011	decreased expression levels	985:1011	decreased expression levels of inflammatory cytokines	985:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	8	theme	increased	823:831	arg1	level					844:848	an increased expression level	820:848	an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines	820:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	10	9	theme	longum	1632:1637	arg1	subsp					1639:1643	Bifidobacterium longum subsp	1616:1643	Bifidobacterium longum subsp	1616:1643	Bifidobacterium bifidum E3 combined with Bifidobacterium longum subsp.
37255290	5	10	theme	BLBB	714:717	arg1	group					719:723	the BLBB group	710:723	the BLBB group	710:723	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	5	11	from	levels	630:635	arg1	serum					687:691	the serum	683:691	the serum	683:691	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	2	12	with	parallel	248:255	arg1	development					266:276	the development	262:276	the development of sepsis	262:286	Changes in the functions of the intestinal barrier occur in parallel with the development of sepsis.
37255290	9	13	theme	microbiota	1563:1572	arg1	composition					1544:1554	the composition	1540:1554	the composition of gut microbiota	1540:1572	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	4	14	theme	cells	619:623	arg1	number					569:574	the number	565:574	the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells	565:623	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	2	15	theme	intestinal	220:229	arg1	barrier					231:237	the intestinal barrier	216:237	the intestinal barrier	216:237	Changes in the functions of the intestinal barrier occur in parallel with the development of sepsis.
37255290	6	16	theme	zonula	878:883	arg1	proteins					868:875	tight junction proteins	853:875	tight junction proteins (zonula occludens-1, occludin, and claudin-1)	853:921	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	16	theme	zonula	878:883	arg1	occludens-1					885:895	zonula occludens-1	878:895	zonula occludens-1	878:895	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	4	17	theme	T	602:602	arg1	number					569:574	the number	565:574	the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells	565:623	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	1	18	theme	Signaling	160:168	arg1	Pathways					170:177	MAPK Signaling Pathways	155:177	MAPK Signaling Pathways	155:177	infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo.
37255290	6	19	theme	proteins	868:875	arg1	IL-1β					1040:1044	IL-1β	1040:1044	IL-1β	1040:1044	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	19	theme	proteins	868:875	arg1	level					844:848	an increased expression level	820:848	an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines	820:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	19	theme	proteins	868:875	arg1	IL-6					1047:1050	IL-6	1047:1050	IL-6	1047:1050	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	19	theme	proteins	868:875	arg1	TNF-α					1057:1061	TNF-α	1057:1061	TNF-α	1057:1061	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	8	20	theme	deoxycholic	1322:1332	arg1	acid					1334:1337	deoxycholic acid	1322:1337	deoxycholic acid	1322:1337	Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate.
37255290	5	21	theme	LPS	771:773	arg1	group					775:779	the LPS group	767:779	the LPS group	767:779	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	6	22	theme	tight	853:857	arg1	claudin-1					912:920	claudin-1	912:920	claudin-1	912:920	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	22	theme	tight	853:857	arg1	occludin					898:905	occludin	898:905	occludin	898:905	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	22	theme	tight	853:857	arg1	proteins					868:875	tight junction proteins	853:875	tight junction proteins (zonula occludens-1, occludin, and claudin-1)	853:921	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	22	theme	tight	853:857	arg1	occludens-1					885:895	zonula occludens-1	878:895	zonula occludens-1	878:895	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	4	23	theme	ileal	467:471	arg1	length					480:485	ileal villus length	467:485	ileal villus length	467:485	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	1	24	theme	infantis	71:78	arg1	E4					80:81	infantis E4	71:81	infantis E4	71:81	infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo.
37255290	6	25	dep	proteins	868:875	arg1	proteins					868:875	tight junction proteins	853:875	tight junction proteins (zonula occludens-1, occludin, and claudin-1)	853:921	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	25	dep	proteins	868:875	arg1	occludin					898:905	occludin	898:905	occludin	898:905	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	25	dep	proteins	868:875	arg1	claudin-1					912:920	claudin-1	912:920	claudin-1	912:920	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	25	dep	proteins	868:875	arg1	occludens-1					885:895	zonula occludens-1	878:895	zonula occludens-1	878:895	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	5	26	theme	oxidase	648:654	arg1	levels					630:635	The levels	626:635	The levels of diamine oxidase (DAO) and d-lactic acid in the serum	626:691	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	9	27	theme	BLBB	1399:1402	arg1	group					1404:1408	the BLBB group	1395:1408	the BLBB group	1395:1408	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	0	28	theme	Bifidobacterium	0:14	arg1	E3					24:25	Bifidobacterium bifidum E3	0:25	Bifidobacterium bifidum E3	0:25	Bifidobacterium bifidum E3 Combined with Bifidobacterium longum subsp.
37255290	1	29	theme	Intestinal	104:113	arg1	Injury					115:120	LPS-Induced Intestinal Injury	92:120	LPS-Induced Intestinal Injury	92:120	infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo.
37255290	7	30	from	increase	1213:1220	arg1	strains					1225:1231	strains	1225:1231	strains producing short-chain fatty acids	1225:1265	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	4	31	theme	BLBB	509:512	arg1	group					514:518	the BLBB group	505:518	the BLBB group	505:518	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	5	32	theme	acid	675:678	arg1	levels					630:635	The levels	626:635	The levels of diamine oxidase (DAO) and d-lactic acid in the serum	626:691	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	4	33	theme	length	480:485	arg1	ratio					458:462	the ratio	454:462	the ratio of ileal villus length to crypt depth	454:500	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	0	34	theme	longum	57:62	arg1	subsp					64:68	Bifidobacterium longum subsp	41:68	Bifidobacterium longum subsp	41:68	Bifidobacterium bifidum E3 Combined with Bifidobacterium longum subsp.
37255290	9	35	theme	intestinal	1444:1453	arg1	barrier					1455:1461	the intestinal barrier	1440:1461	the intestinal barrier	1440:1461	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	6	36	theme	NFκB/MAPK	951:959	arg1	pathways					971:978	reduced NFκB/MAPK signaling pathways	943:978	reduced NFκB/MAPK signaling pathways	943:978	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	37	theme	cytokines	1029:1037	arg1	occludin					898:905	occludin	898:905	occludin	898:905	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	37	theme	cytokines	1029:1037	arg1	levels					1006:1011	decreased expression levels	985:1011	decreased expression levels of inflammatory cytokines	985:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	37	theme	cytokines	1029:1037	arg1	proteins					868:875	tight junction proteins	853:875	tight junction proteins (zonula occludens-1, occludin, and claudin-1)	853:921	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	37	theme	cytokines	1029:1037	arg1	pathways					971:978	reduced NFκB/MAPK signaling pathways	943:978	reduced NFκB/MAPK signaling pathways	943:978	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	37	theme	cytokines	1029:1037	arg1	mRNA					937:940	mucin (MUC2) mRNA	924:940	mucin (MUC2) mRNA	924:940	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	37	theme	cytokines	1029:1037	arg1	claudin-1					912:920	claudin-1	912:920	claudin-1	912:920	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	37	theme	cytokines	1029:1037	arg1	occludens-1					885:895	zonula occludens-1	878:895	zonula occludens-1	878:895	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	10	38	theme	bifidum	1591:1597	arg1	E3					1599:1600	Bifidobacterium bifidum E3	1575:1600	Bifidobacterium bifidum E3	1575:1600	Bifidobacterium bifidum E3 combined with Bifidobacterium longum subsp.
37255290	9	39	theme	mucosal	1478:1484	arg1	immunity					1486:1493	intestinal mucosal immunity	1467:1493	intestinal mucosal immunity	1467:1493	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	7	40	theme	BLBB	1069:1072	arg1	group					1074:1078	The BLBB group	1065:1078	The BLBB group	1065:1078	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	2	41	from	Changes	188:194	arg1	functions					203:211	the functions	199:211	the functions of the intestinal barrier	199:237	Changes in the functions of the intestinal barrier occur in parallel with the development of sepsis.
37255290	7	42	theme	short-chain	1243:1253	arg1	acids					1261:1265	short-chain fatty acids	1243:1265	short-chain fatty acids	1243:1265	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	9	43	theme	intestinal	1503:1512	arg1	inflammation					1514:1525	intestinal inflammation	1503:1525	intestinal inflammation	1503:1525	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	2	44	theme	sepsis	281:286	arg1	development					266:276	the development	262:276	the development of sepsis	262:286	Changes in the functions of the intestinal barrier occur in parallel with the development of sepsis.
37255290	5	45	theme	LPS	731:733	arg1	injection					735:743	LPS injection	731:743	LPS injection	731:743	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	6	46	theme	expression	995:1004	arg1	levels					1006:1011	decreased expression levels	985:1011	decreased expression levels of inflammatory cytokines	985:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	4	47	from	increase	442:449	arg1	ratio					458:462	the ratio	454:462	the ratio of ileal villus length to crypt depth	454:500	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	4	47	from	increase	442:449	arg1	group					514:518	the BLBB group	505:518	the BLBB group	505:518	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	8	48	theme	biosynthesized	1343:1356	arg1	linoleate					1358:1366	biosynthesized linoleate	1343:1366	biosynthesized linoleate	1343:1366	Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate.
37255290	6	49	theme	expression	833:842	arg1	level					844:848	an increased expression level	820:848	an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines	820:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	7	50	theme	fatty	1255:1259	arg1	acids					1261:1265	short-chain fatty acids	1243:1265	short-chain fatty acids	1243:1265	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	4	51	theme	plasma	584:589	arg1	number					569:574	the number	565:574	the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells	565:623	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	8	52	theme	BLBB	1285:1288	arg1	group					1290:1294	the BLBB group	1281:1294	the BLBB group	1281:1294	Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate.
37255290	6	53	theme	pathways	971:978	arg1	IL-1β					1040:1044	IL-1β	1040:1044	IL-1β	1040:1044	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	53	theme	pathways	971:978	arg1	level					844:848	an increased expression level	820:848	an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines	820:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	53	theme	pathways	971:978	arg1	IL-6					1047:1050	IL-6	1047:1050	IL-6	1047:1050	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	53	theme	pathways	971:978	arg1	TNF-α					1057:1061	TNF-α	1057:1061	TNF-α	1057:1061	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	7	54	theme	relative	1093:1100	arg1	abundance					1102:1110	the relative abundance	1089:1110	the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes	1089:1194	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	9	55	theme	gut	1559:1561	arg1	microbiota					1563:1572	gut microbiota	1559:1572	gut microbiota	1559:1572	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	4	56	theme	crypt	490:494	arg1	depth					496:500	crypt depth	490:500	crypt depth	490:500	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	2	57	theme	barrier	231:237	arg1	functions					203:211	the functions	199:211	the functions of the intestinal barrier	199:237	Changes in the functions of the intestinal barrier occur in parallel with the development of sepsis.
37255290	8	58	theme	higher	1305:1310	arg1	levels					1312:1317	higher levels	1305:1317	higher levels of deoxycholic acid and biosynthesized linoleate	1305:1366	Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate.
37255290	4	59	theme	dendritic	609:617	arg1	cells					619:623	dendritic cells	609:623	dendritic cells	609:623	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	1	60	theme	MAPK	155:158	arg1	Pathways					170:177	MAPK Signaling Pathways	155:177	MAPK Signaling Pathways	155:177	infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo.
37255290	8	61	theme	acid	1334:1337	arg1	levels					1312:1317	higher levels	1305:1317	higher levels of deoxycholic acid and biosynthesized linoleate	1305:1366	Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate.
37255290	7	62	theme	Alistipes	1186:1194	arg1	abundance					1102:1110	the relative abundance	1089:1110	the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes	1089:1194	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	4	63	theme	CD4+/CD8+	592:600	arg1	T					602:602	CD4+/CD8+ T	592:602	CD4+/CD8+ T	592:602	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	6	64	theme	junction	859:866	arg1	claudin-1					912:920	claudin-1	912:920	claudin-1	912:920	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	64	theme	junction	859:866	arg1	occludin					898:905	occludin	898:905	occludin	898:905	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	64	theme	junction	859:866	arg1	proteins					868:875	tight junction proteins	853:875	tight junction proteins (zonula occludens-1, occludin, and claudin-1)	853:921	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	64	theme	junction	859:866	arg1	occludens-1					885:895	zonula occludens-1	878:895	zonula occludens-1	878:895	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	4	65	theme	villus	473:478	arg1	length					480:485	ileal villus length	467:485	ileal villus length	467:485	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	11	66	theme	infantis	1646:1653	arg1	E4					1655:1656	infantis E4	1646:1656	infantis E4	1646:1656	infantis E4 may thus serve as a probiotic against the intestinal injury caused by LPS.
37255290	11	66	theme	infantis	1646:1653	arg1	probiotic					1678:1686	probiotic	1678:1686	probiotic	1678:1686	infantis E4 may thus serve as a probiotic against the intestinal injury caused by LPS.
37255290	5	67	theme	diamine	640:646	arg1	DAO					657:659	DAO	657:659	DAO	657:659	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	5	67	theme	diamine	640:646	arg1	oxidase					648:654	diamine oxidase	640:654	diamine oxidase (DAO)	640:660	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	6	68	theme	mucin	924:928	arg1	mRNA					937:940	mucin (MUC2) mRNA	924:940	mucin (MUC2) mRNA	924:940	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	0	69	theme	bifidum	16:22	arg1	E3					24:25	Bifidobacterium bifidum E3	0:25	Bifidobacterium bifidum E3	0:25	Bifidobacterium bifidum E3 Combined with Bifidobacterium longum subsp.
37255290	1	70	theme	LPS-Induced	92:102	arg1	Injury					115:120	LPS-Induced Intestinal Injury	92:120	LPS-Induced Intestinal Injury	92:120	infantis E4 Improves LPS-Induced Intestinal Injury by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways In Vivo.
37255290	8	71	theme	linoleate	1358:1366	arg1	levels					1312:1317	higher levels	1305:1317	higher levels of deoxycholic acid and biosynthesized linoleate	1305:1366	Furthermore, the BLBB group leads to higher levels of deoxycholic acid and biosynthesized linoleate.
37255290	7	72	theme	Lachnospiraceae_NK4A136_group	1131:1159	arg1	abundance					1102:1110	the relative abundance	1089:1110	the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes	1089:1194	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	7	73	theme	Muribaculaceae	1115:1128	arg1	abundance					1102:1110	the relative abundance	1089:1110	the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes	1089:1194	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	5	74	theme	d-lactic	666:673	arg1	acid					675:678	d-lactic acid	666:678	d-lactic acid	666:678	The levels of diamine oxidase (DAO) and d-lactic acid in the serum were lessened in the BLBB group after LPS injection compared with that in the LPS group.
37255290	4	75	dep	number	569:574	arg1	as					557:558	as	557:558	as	557:558	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	4	75	dep	number	569:574	arg1	were					560:563	were	560:563	were	560:563	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	7	76	theme	Clostridia_Ucg-014	1162:1179	arg1	abundance					1102:1110	the relative abundance	1089:1110	the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes	1089:1194	The BLBB group enriched the relative abundance of Muribaculaceae, Lachnospiraceae_NK4A136_group, Clostridia_Ucg-014, and Alistipes, resulting in an increase in strains producing short-chain fatty acids.
37255290	6	77	theme	signaling	961:969	arg1	pathways					971:978	reduced NFκB/MAPK signaling pathways	943:978	reduced NFκB/MAPK signaling pathways	943:978	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	9	78	theme	barrier	1455:1461	arg1	capacity					1428:1435	the capacity	1424:1435	the capacity of the intestinal barrier and intestinal mucosal immunity	1424:1493	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	6	79	theme	reduced	943:949	arg1	pathways					971:978	reduced NFκB/MAPK signaling pathways	943:978	reduced NFκB/MAPK signaling pathways	943:978	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	4	80	theme	LPS	546:548	arg1	group					550:554	the LPS group	542:554	the LPS group	542:554	The results revealed an increase in the ratio of ileal villus length to crypt depth in the BLBB group compared with that in the LPS group, as were the number of IgA+ plasma, CD4+/CD8+ T, and dendritic cells.
37255290	11	81	theme	intestinal	1700:1709	arg1	injury					1711:1716	the intestinal injury	1696:1716	the intestinal injury caused by LPS	1696:1730	infantis E4 may thus serve as a probiotic against the intestinal injury caused by LPS.
37255290	9	82	theme	intestinal	1467:1476	arg1	immunity					1486:1493	intestinal mucosal immunity	1467:1493	intestinal mucosal immunity	1467:1493	This study suggested that the BLBB group could enhance the capacity of the intestinal barrier and intestinal mucosal immunity, reduce intestinal inflammation, and improve the composition of gut microbiota.
37255290	6	83	theme	mRNA	937:940	arg1	IL-1β					1040:1044	IL-1β	1040:1044	IL-1β	1040:1044	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	83	theme	mRNA	937:940	arg1	level					844:848	an increased expression level	820:848	an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines	820:1037	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	83	theme	mRNA	937:940	arg1	IL-6					1047:1050	IL-6	1047:1050	IL-6	1047:1050	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	6	83	theme	mRNA	937:940	arg1	TNF-α					1057:1061	TNF-α	1057:1061	TNF-α	1057:1061	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37255290	10	84	theme	Bifidobacterium	1575:1589	arg1	E3					1599:1600	Bifidobacterium bifidum E3	1575:1600	Bifidobacterium bifidum E3	1575:1600	Bifidobacterium bifidum E3 combined with Bifidobacterium longum subsp.
37255290	6	85	theme	BLBB	799:802	arg1	group					804:808	the BLBB group	795:808	the BLBB group	795:808	In addition, the BLBB group exhibited an increased expression level of tight junction proteins (zonula occludens-1, occludin, and claudin-1), mucin (MUC2) mRNA, reduced NFκB/MAPK signaling pathways, and decreased expression levels of inflammatory cytokines (IL-1β, IL-6, and TNF-α).
37311500	10	0	theme	docking	1513:1519	arg1	study					1521:1525	The molecular docking study	1499:1525	The molecular docking study for (E)-cinnamaldehyde and NAAA	1499:1557	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	18	1	theme	main	2966:2969	arg1	O-methoxycinnamaldehyde					2894:2916	O-methoxycinnamaldehyde	2894:2916	O-methoxycinnamaldehyde	2894:2916	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	18	1	theme	main	2966:2969	arg1	-cinnamaldehyde					2874:2888	-cinnamaldehyde	2874:2888	-cinnamaldehyde	2874:2888	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	18	1	theme	main	2966:2969	arg1	contributors					2971:2982	the main contributors	2962:2982	the main contributors of the anti-inflammatory effects of RCEO	2962:3023	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	12	2	theme	NAAA	1952:1955	arg1	activity					1957:1964	the NAAA activity	1948:1964	the NAAA activity in NAAA-overexpressing HEK293 cells	1948:2000	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	5	3	dep	detected	910:917	arg1	which					867:871	which	867:871	which	867:871	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	3	dep	detected	910:917	arg1	both					859:862	both	859:862	both	859:862	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	3	dep	detected	910:917	arg1	substrates					893:902	NAAA endogenous substrates	877:902	NAAA endogenous substrates	877:902	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	3	4	theme	N-acylethanolamine	554:571	arg1	amidase					578:584	N-acylethanolamine acid amidase	554:584	N-acylethanolamine acid amidase (NAAA)	554:591	AIM OF THE STUDY To investigate whether N-acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO.
37311500	3	4	theme	N-acylethanolamine	554:571	arg1	NAAA					587:590	NAAA	587:590	NAAA	587:590	AIM OF THE STUDY To investigate whether N-acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO.
37311500	14	5	theme	E	2215:2215	arg1	-cinnamaldehyde					2217:2231	(E)-cinnamaldehyde	2214:2231	(E)-cinnamaldehyde	2214:2231	Interestingly, the GC-MS assay revealed that more than 93 components were identified in RCEO, of which (E)-cinnamaldehyde accounted for 64.88%.
37311500	5	6	theme	liquid	922:927	arg1	chromatography					929:942	liquid chromatography	922:942	liquid chromatography with tandem mass spectrometry (HPLC-MS/MS)	922:985	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	18	7	from	components	2923:2932	arg1	RCEO					2937:2940	RCEO	2937:2940	RCEO	2937:2940	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	5	8	theme	NAAA	877:880	arg1	which					867:871	which	867:871	which	867:871	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	8	theme	NAAA	877:880	arg1	both					859:862	both	859:862	both	859:862	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	8	theme	NAAA	877:880	arg1	substrates					893:902	NAAA endogenous substrates	877:902	NAAA endogenous substrates	877:902	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	12	9	theme	OEA	1930:1932	arg1	degradation					1898:1908	the degradation	1894:1908	the degradation of cellular PEA and OEA	1894:1932	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	4	10	theme	steam	685:689	arg1	distillation					691:702	steam distillation	685:702	steam distillation of Ramulus Cinnamomi	685:723	MATERIALS AND METHODS RCEO was extracted by steam distillation of Ramulus Cinnamomi, and NAAA activity was detected using HEK293 cells overexpressing NAAA.
37311500	9	11	theme	RCEO	1432:1435	arg1	composition					1417:1427	The chemical composition	1404:1427	The chemical composition of RCEO	1404:1435	The chemical composition of RCEO was assessed by gas chromatography-mass spectroscopy (GC-MS).
37311500	6	12	theme	CCK-8	1159:1163	arg1	kit					1166:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	14	13	theme	GC-MS	2130:2134	arg1	assay					2136:2140	the GC-MS assay	2126:2140	the GC-MS assay	2126:2140	Interestingly, the GC-MS assay revealed that more than 93 components were identified in RCEO, of which (E)-cinnamaldehyde accounted for 64.88%.
37311500	10	14	theme	Studio	1592:1597	arg1	DS2019					1614:1619	DS2019	1614:1619	DS2019	1614:1619	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	10	14	theme	Studio	1592:1597	arg1	software					1604:1611	Discovery Studio 2019 software	1582:1611	Discovery Studio 2019 software (DS2019)	1582:1620	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	13	15	theme	-stimulated	2086:2096	arg1	macrophages					2098:2108	lipopolysaccharide (LPS)-stimulated macrophages	2062:2108	lipopolysaccharide (LPS)-stimulated macrophages	2062:2108	In addition, RCEO also decreased NO and TNF-α cytokines in lipopolysaccharide (LPS)-stimulated macrophages.
37311500	3	16	theme	anti-inflammatory	606:622	arg1	effects					624:630	the anti-inflammatory effects	602:630	the anti-inflammatory effects of RCEO	602:638	AIM OF THE STUDY To investigate whether N-acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO.
37311500	15	17	theme	key	2443:2445	arg1	components					2447:2456	key components	2443:2456	key components of RCEO that inhibit NAAA activity	2443:2491	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	9	18	theme	gas	1453:1455	arg1	GC-MS					1491:1495	GC-MS	1491:1495	GC-MS	1491:1495	The chemical composition of RCEO was assessed by gas chromatography-mass spectroscopy (GC-MS).
37311500	9	18	theme	gas	1453:1455	arg1	spectroscopy					1477:1488	gas chromatography-mass spectroscopy	1453:1488	gas chromatography-mass spectroscopy (GC-MS)	1453:1496	The chemical composition of RCEO was assessed by gas chromatography-mass spectroscopy (GC-MS).
37311500	12	19	theme	PEA	1922:1924	arg1	degradation					1898:1908	the degradation	1894:1908	the degradation of cellular PEA and OEA	1894:1932	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	18	20	theme	anti-inflammatory	2991:3007	arg1	effects					3009:3015	the anti-inflammatory effects	2987:3015	the anti-inflammatory effects of RCEO	2987:3023	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	8	21	theme	tumor	1268:1272	arg1	TNF-α					1293:1297	TNF-α	1293:1297	TNF-α	1293:1297	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	8	21	theme	tumor	1268:1272	arg1	factor-α					1283:1290	tumor necrosis factor-α	1268:1290	tumor necrosis factor-α (TNF-α)	1268:1298	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	16	22	theme	NAAA	2586:2589	arg1	cavity					2576:2581	the catalytic cavity	2562:2581	the catalytic cavity of NAAA	2562:2589	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	8	23	theme	factor-α	1283:1290	arg1	level					1259:1263	The level	1255:1263	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant	1255:1331	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	8	24	from	level	1259:1263	arg1	supernatant					1321:1331	the RAW264.7 cell supernatant	1303:1331	the RAW264.7 cell supernatant	1303:1331	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	5	25	theme	tandem	949:954	arg1	HPLC-MS/MS					975:984	HPLC-MS/MS	975:984	HPLC-MS/MS	975:984	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	25	theme	tandem	949:954	arg1	spectrometry					961:972	tandem mass spectrometry	949:972	tandem mass spectrometry (HPLC-MS/MS)	949:985	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	15	26	theme	3.21 ± 0.03	2374:2384	arg1	IC50					2366:2369	an IC50	2363:2369	an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL	2363:2406	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	4	27	theme	Cinnamomi	715:723	arg1	distillation					691:702	steam distillation	685:702	steam distillation of Ramulus Cinnamomi	685:723	MATERIALS AND METHODS RCEO was extracted by steam distillation of Ramulus Cinnamomi, and NAAA activity was detected using HEK293 cells overexpressing NAAA.
37311500	7	28	theme	cell	1200:1203	arg1	supernatant					1205:1215	the cell supernatant	1196:1215	the cell supernatant	1196:1215	The nitric oxide (NO) in the cell supernatant was measured using the Griess method.
37311500	18	29	theme	NAAA	3067:3070	arg1	inhibition					3072:3081	NAAA inhibition	3067:3081	NAAA inhibition	3067:3081	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	6	30	theme	RCEO	1021:1024	arg1	effects					1010:1016	The anti-inflammatory effects	988:1016	The anti-inflammatory effects of RCEO	988:1024	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	8	31	theme	RAW264.7 cell	1307:1319	arg1	supernatant					1321:1331	the RAW264.7 cell supernatant	1303:1331	the RAW264.7 cell supernatant	1303:1331	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	0	32	theme	acid	112:115	arg1	inhibition					125:134	N-acylethanolamine acid amidase inhibition	93:134	N-acylethanolamine acid amidase inhibition	93:134	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	15	33	theme	NAAA	2479:2482	arg1	activity					2484:2491	NAAA activity	2479:2491	NAAA activity	2479:2491	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	4	34	theme	NAAA	730:733	arg1	activity					735:742	NAAA activity	730:742	NAAA activity	730:742	MATERIALS AND METHODS RCEO was extracted by steam distillation of Ramulus Cinnamomi, and NAAA activity was detected using HEK293 cells overexpressing NAAA.
37311500	17	35	theme	PEA	2815:2817	arg1	levels					2827:2832	cellular PEA and OEA levels	2806:2832	cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells	2806:2868	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	7	36	from	oxide	1182:1186	arg1	supernatant					1205:1215	the cell supernatant	1196:1215	the cell supernatant	1196:1215	The nitric oxide (NO) in the cell supernatant was measured using the Griess method.
37311500	17	37	from	levels	2827:2832	arg1	HEK293 cells					2857:2868	NAAA-overexpressing HEK293 cells	2837:2868	NAAA-overexpressing HEK293 cells	2837:2868	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	2	38	dep	Cinnamomi	345:353	arg1	RCEO					370:373	RCEO	370:373	RCEO	370:373	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	2	38	dep	Cinnamomi	345:353	arg1	oil					365:367	essential oil	355:367	Ramulus Cinnamomi essential oil (RCEO)	337:374	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	12	39	from	activity	1957:1964	arg1	HEK293 cells					1989:2000	NAAA-overexpressing HEK293 cells	1969:2000	NAAA-overexpressing HEK293 cells	1969:2000	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	0	40	theme	Ramulus	0:6	arg1	oil					28:30	Ramulus Cinnamomi essential oil	0:30	Ramulus Cinnamomi essential oil	0:30	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	18	41	theme	effects	3009:3015	arg1	O-methoxycinnamaldehyde					2894:2916	O-methoxycinnamaldehyde	2894:2916	O-methoxycinnamaldehyde	2894:2916	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	18	41	theme	effects	3009:3015	arg1	-cinnamaldehyde					2874:2888	-cinnamaldehyde	2874:2888	-cinnamaldehyde	2874:2888	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	18	41	theme	effects	3009:3015	arg1	contributors					2971:2982	the main contributors	2962:2982	the main contributors of the anti-inflammatory effects of RCEO	2962:3023	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	16	42	theme	hydrophobic-related	2654:2672	arg1	interactions					2674:2685	hydrophobic-related interactions	2654:2685	hydrophobic-related interactions with LEU152 of human NAAA	2654:2711	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	17	43	theme	CONCLUSIONS	2714:2724	arg1	RCEO					2726:2729	CONCLUSIONS RCEO	2714:2729	CONCLUSIONS RCEO	2714:2729	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	8	44	theme	enzyme-linked	1357:1369	arg1	ELISA					1392:1396	ELISA	1392:1396	ELISA	1392:1396	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	8	44	theme	enzyme-linked	1357:1369	arg1	assay					1385:1389	an enzyme-linked immunosorbent assay	1354:1389	an enzyme-linked immunosorbent assay (ELISA) kit	1354:1401	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	0	45	theme	essential	18:26	arg1	oil					28:30	Ramulus Cinnamomi essential oil	0:30	Ramulus Cinnamomi essential oil	0:30	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	12	46	theme	OEA	1811:1813	arg1	levels					1815:1820	PEA and OEA levels	1803:1820	PEA and OEA levels in NAAA-overexpressing HEK293 cells	1803:1856	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	6	47	theme	-stimulated	1067:1077	arg1	RAW264.7 cells					1079:1092	lipopolysaccharide (LPS)-stimulated RAW264.7 cells	1043:1092	lipopolysaccharide (LPS)-stimulated RAW264.7 cells	1043:1092	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	8	48	theme	assay	1385:1389	arg1	kit					1399:1401	an enzyme-linked immunosorbent assay (ELISA) kit	1354:1401	an enzyme-linked immunosorbent assay (ELISA) kit	1354:1401	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	18	49	theme	cellular	3039:3046	arg1	levels					3052:3057	cellular PEA levels	3039:3057	cellular PEA levels	3039:3057	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	2	50	theme	potential	410:418	arg1	mechanisms					420:429	the potential mechanisms	406:429	the potential mechanisms by which RCEO exerts its anti-inflammatory effects	406:480	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	10	51	theme	molecular	1503:1511	arg1	study					1521:1525	The molecular docking study	1499:1525	The molecular docking study for (E)-cinnamaldehyde and NAAA	1499:1557	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	11	52	theme	cell	1648:1651	arg1	model					1653:1657	a cell model	1646:1657	a cell model for evaluating NAAA activity	1646:1686	RESULTS We established a cell model for evaluating NAAA activity, and we found that RCEO inhibited the NAAA activity with an IC50 of 5.64 ± 0.62 μg/mL.
37311500	17	53	theme	OEA	2823:2825	arg1	levels					2827:2832	cellular PEA and OEA levels	2806:2832	cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells	2806:2868	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	0	54	theme	anti-inflammatory	42:58	arg1	effect					60:65	an anti-inflammatory effect	39:65	an anti-inflammatory effect on RAW264.7 cells	39:83	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	1	55	theme	ETHNOPHARMACOLOGICAL	137:156	arg1	RELEVANCE					158:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	55	theme	ETHNOPHARMACOLOGICAL	137:156	arg1	medicine					263:270	a traditional Chinese medicine	241:270	a traditional Chinese medicine (TCM) with anti-inflammatory effects	241:307	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	55	theme	ETHNOPHARMACOLOGICAL	137:156	arg1	twig					197:200	the dried twig	187:200	the dried twig of Cinnamomum cassia (L.) J.Presl.	187:235	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	56	with	medicine	263:270	arg1	effects					301:307	anti-inflammatory effects	283:307	anti-inflammatory effects	283:307	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	6	57	theme	Counting	1143:1150	arg1	kit					1166:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	2	58	theme	Cinnamomi	345:353	arg1	functions					324:332	The medicinal functions	310:332	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO)	310:374	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	12	59	theme	PEA	1803:1805	arg1	levels					1815:1820	PEA and OEA levels	1803:1820	PEA and OEA levels in NAAA-overexpressing HEK293 cells	1803:1856	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	11	60	theme	NAAA	1674:1677	arg1	activity					1679:1686	NAAA activity	1674:1686	NAAA activity	1674:1686	RESULTS We established a cell model for evaluating NAAA activity, and we found that RCEO inhibited the NAAA activity with an IC50 of 5.64 ± 0.62 μg/mL.
37311500	1	61	theme	traditional	243:253	arg1	TCM					273:275	TCM	273:275	TCM	273:275	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	61	theme	traditional	243:253	arg1	RELEVANCE					158:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	61	theme	traditional	243:253	arg1	medicine					263:270	a traditional Chinese medicine	241:270	a traditional Chinese medicine (TCM) with anti-inflammatory effects	241:307	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	12	62	theme	NAAA-overexpressing	1969:1987	arg1	HEK293 cells					1989:2000	NAAA-overexpressing HEK293 cells	1969:2000	NAAA-overexpressing HEK293 cells	1969:2000	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	7	63	theme	Griess	1240:1245	arg1	method					1247:1252	the Griess method	1236:1252	the Griess method	1236:1252	The nitric oxide (NO) in the cell supernatant was measured using the Griess method.
37311500	5	64	theme	endogenous	882:891	arg1	which					867:871	which	867:871	which	867:871	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	64	theme	endogenous	882:891	arg1	both					859:862	both	859:862	both	859:862	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	64	theme	endogenous	882:891	arg1	substrates					893:902	NAAA endogenous substrates	877:902	NAAA endogenous substrates	877:902	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	9	65	theme	chemical	1408:1415	arg1	composition					1417:1427	The chemical composition	1404:1427	The chemical composition of RCEO	1404:1435	The chemical composition of RCEO was assessed by gas chromatography-mass spectroscopy (GC-MS).
37311500	18	66	theme	RCEO	3020:3023	arg1	effects					3009:3015	the anti-inflammatory effects	2987:3015	the anti-inflammatory effects of RCEO	2987:3023	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	1	67	theme	cassia	216:221	arg1	RELEVANCE					158:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	67	theme	cassia	216:221	arg1	twig					197:200	the dried twig	187:200	the dried twig of Cinnamomum cassia (L.) J.Presl.	187:235	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	6	68	theme	anti-inflammatory	992:1008	arg1	effects					1010:1016	The anti-inflammatory effects	988:1016	The anti-inflammatory effects of RCEO	988:1024	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	2	69	theme	medicinal	314:322	arg1	functions					324:332	The medicinal functions	310:332	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO)	310:374	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	12	70	from	levels	1815:1820	arg1	HEK293 cells					1845:1856	NAAA-overexpressing HEK293 cells	1825:1856	NAAA-overexpressing HEK293 cells	1825:1856	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	4	71	theme	MATERIALS	641:649	arg1	RCEO					663:666	MATERIALS AND METHODS RCEO	641:666	MATERIALS AND METHODS RCEO	641:666	MATERIALS AND METHODS RCEO was extracted by steam distillation of Ramulus Cinnamomi, and NAAA activity was detected using HEK293 cells overexpressing NAAA.
37311500	10	72	theme	2019	1599:1602	arg1	DS2019					1614:1619	DS2019	1614:1619	DS2019	1614:1619	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	10	72	theme	2019	1599:1602	arg1	software					1604:1611	Discovery Studio 2019 software	1582:1611	Discovery Studio 2019 software (DS2019)	1582:1620	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	15	73	theme	NAAA	2344:2347	arg1	activity					2349:2356	NAAA activity	2344:2356	NAAA activity	2344:2356	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	8	74	link	enzyme-linked	1357:1369	arg1	ELISA					1392:1396	ELISA	1392:1396	ELISA	1392:1396	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	8	74	link	enzyme-linked	1357:1369	arg1	assay					1385:1389	an enzyme-linked immunosorbent assay	1354:1389	an enzyme-linked immunosorbent assay (ELISA) kit	1354:1401	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	16	75	theme	NAAA	2708:2711	arg1	LEU152					2692:2697	LEU152	2692:2697	LEU152 of human NAAA	2692:2711	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	4	76	theme	METHODS	655:661	arg1	RCEO					663:666	MATERIALS AND METHODS RCEO	641:666	MATERIALS AND METHODS RCEO	641:666	MATERIALS AND METHODS RCEO was extracted by steam distillation of Ramulus Cinnamomi, and NAAA activity was detected using HEK293 cells overexpressing NAAA.
37311500	10	77	theme	Discovery	1582:1590	arg1	DS2019					1614:1619	DS2019	1614:1619	DS2019	1614:1619	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	10	77	theme	Discovery	1582:1590	arg1	software					1604:1611	Discovery Studio 2019 software	1582:1611	Discovery Studio 2019 software (DS2019)	1582:1620	The molecular docking study for (E)-cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019).
37311500	9	78	theme	chromatography-mass	1457:1475	arg1	GC-MS					1491:1495	GC-MS	1491:1495	GC-MS	1491:1495	The chemical composition of RCEO was assessed by gas chromatography-mass spectroscopy (GC-MS).
37311500	9	78	theme	chromatography-mass	1457:1475	arg1	spectroscopy					1477:1488	gas chromatography-mass spectroscopy	1453:1488	gas chromatography-mass spectroscopy (GC-MS)	1453:1496	The chemical composition of RCEO was assessed by gas chromatography-mass spectroscopy (GC-MS).
37311500	12	79	theme	cellular	1913:1920	arg1	PEA					1922:1924	cellular PEA	1913:1924	cellular PEA	1913:1924	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	11	80	theme	5.64 ± 0.62 μg/mL	1756:1772	arg1	IC50					1748:1751	an IC50	1745:1751	an IC50 of 5.64 ± 0.62 μg/mL	1745:1772	RESULTS We established a cell model for evaluating NAAA activity, and we found that RCEO inhibited the NAAA activity with an IC50 of 5.64 ± 0.62 μg/mL.
37311500	3	81	theme	RCEO	635:638	arg1	effects					624:630	the anti-inflammatory effects	602:630	the anti-inflammatory effects of RCEO	602:638	AIM OF THE STUDY To investigate whether N-acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO.
37311500	18	82	theme	PEA	3048:3050	arg1	levels					3052:3057	cellular PEA levels	3039:3057	cellular PEA levels	3039:3057	(E)-cinnamaldehyde and O-methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
37311500	3	83	theme	acid	573:576	arg1	amidase					578:584	N-acylethanolamine acid amidase	554:584	N-acylethanolamine acid amidase (NAAA)	554:591	AIM OF THE STUDY To investigate whether N-acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO.
37311500	3	83	theme	acid	573:576	arg1	NAAA					587:590	NAAA	587:590	NAAA	587:590	AIM OF THE STUDY To investigate whether N-acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO.
37311500	16	84	theme	docking	2505:2511	arg1	assays					2513:2518	docking assays	2505:2518	docking assays	2505:2518	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	8	85	theme	necrosis	1274:1281	arg1	TNF-α					1293:1297	TNF-α	1293:1297	TNF-α	1293:1297	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	8	85	theme	necrosis	1274:1281	arg1	factor-α					1283:1290	tumor necrosis factor-α	1268:1290	tumor necrosis factor-α (TNF-α)	1268:1298	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	16	86	theme	hydrogen	2608:2615	arg1	interaction					2622:2632	a hydrogen bond interaction	2606:2632	a hydrogen bond interaction with the TRP181	2606:2648	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	15	87	dep	inhibited	2334:2342	arg1	represent					2433:2441	represent	2433:2441	may represent key components of RCEO that inhibit NAAA activity	2429:2491	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	17	88	theme	NAAA	2778:2781	arg1	activity					2783:2790	NAAA activity	2778:2790	NAAA activity	2778:2790	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	15	89	theme	Further	2255:2261	arg1	experiments					2263:2273	Further experiments	2255:2273	Further experiments	2255:2273	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	0	90	theme	N-acylethanolamine	93:110	arg1	inhibition					125:134	N-acylethanolamine acid amidase inhibition	93:134	N-acylethanolamine acid amidase inhibition	93:134	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	15	91	theme	9.62 ± 0.30 μg/mL	2390:2406	arg1	IC50					2366:2369	an IC50	2363:2369	an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL	2363:2406	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	16	92	theme	bond	2617:2620	arg1	interaction					2622:2632	a hydrogen bond interaction	2606:2632	a hydrogen bond interaction with the TRP181	2606:2648	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	1	93	theme	dried	191:195	arg1	RELEVANCE					158:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	93	theme	dried	191:195	arg1	twig					197:200	the dried twig	187:200	the dried twig of Cinnamomum cassia (L.) J.Presl.	187:235	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	11	94	theme	NAAA	1726:1729	arg1	activity					1731:1738	the NAAA activity	1722:1738	the NAAA activity	1722:1738	RESULTS We established a cell model for evaluating NAAA activity, and we found that RCEO inhibited the NAAA activity with an IC50 of 5.64 ± 0.62 μg/mL.
37311500	0	95	theme	amidase	117:123	arg1	inhibition					125:134	N-acylethanolamine acid amidase inhibition	93:134	N-acylethanolamine acid amidase inhibition	93:134	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	13	96	theme	TNF-α	2043:2047	arg1	cytokines					2049:2057	TNF-α cytokines	2043:2057	TNF-α cytokines	2043:2057	In addition, RCEO also decreased NO and TNF-α cytokines in lipopolysaccharide (LPS)-stimulated macrophages.
37311500	17	97	theme	cellular	2806:2813	arg1	levels					2827:2832	cellular PEA and OEA levels	2806:2832	cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells	2806:2868	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	0	98	from	effect	60:65	arg1	RAW264.7 cells					70:83	RAW264.7 cells	70:83	RAW264.7 cells	70:83	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	17	99	theme	NAAA-overexpressing	2837:2855	arg1	HEK293 cells					2857:2868	NAAA-overexpressing HEK293 cells	2837:2868	NAAA-overexpressing HEK293 cells	2837:2868	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	6	100	theme	cell	1103:1106	arg1	viability					1108:1116	the cell viability	1099:1116	the cell viability	1099:1116	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	16	101	theme	E	2535:2535	arg1	-cinnamaldehyde					2537:2551	(E)-cinnamaldehyde	2534:2551	(E)-cinnamaldehyde	2534:2551	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	1	102	dep	RELEVANCE	158:166	arg1	Cinnamomi					176:184	Cinnamomi	176:184	Cinnamomi	176:184	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	0	103	theme	Cinnamomi	8:16	arg1	oil					28:30	Ramulus Cinnamomi essential oil	0:30	Ramulus Cinnamomi essential oil	0:30	Ramulus Cinnamomi essential oil exerts an anti-inflammatory effect on RAW264.7 cells through N-acylethanolamine acid amidase inhibition.
37311500	5	104	theme	mass	956:959	arg1	HPLC-MS/MS					975:984	HPLC-MS/MS	975:984	HPLC-MS/MS	975:984	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	104	theme	mass	956:959	arg1	spectrometry					961:972	tandem mass spectrometry	949:972	tandem mass spectrometry (HPLC-MS/MS)	949:985	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	105	with	chromatography	929:942	arg1	HPLC-MS/MS					975:984	HPLC-MS/MS	975:984	HPLC-MS/MS	975:984	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	5	105	with	chromatography	929:942	arg1	spectrometry					961:972	tandem mass spectrometry	949:972	tandem mass spectrometry (HPLC-MS/MS)	949:985	N-Palmitoylethanolamide (PEA) and N-oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS).
37311500	1	106	theme	anti-inflammatory	283:299	arg1	effects					301:307	anti-inflammatory effects	283:307	anti-inflammatory effects	283:307	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	15	107	theme	RCEO	2461:2464	arg1	components					2447:2456	key components	2443:2456	key components of RCEO that inhibit NAAA activity	2443:2491	Further experiments showed that (E)-cinnamaldehyde and O-methoxycinnamaldehyde inhibited NAAA activity with an IC50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity.
37311500	12	108	theme	NAAA-overexpressing	1825:1843	arg1	HEK293 cells					1845:1856	NAAA-overexpressing HEK293 cells	1825:1856	NAAA-overexpressing HEK293 cells	1825:1856	RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells.
37311500	16	109	theme	human	2702:2706	arg1	NAAA					2708:2711	human NAAA	2702:2711	human NAAA	2702:2711	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	17	110	theme	anti-inflammatory	2738:2754	arg1	effects					2756:2762	anti-inflammatory effects	2738:2762	anti-inflammatory effects	2738:2762	CONCLUSIONS RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells.
37311500	8	111	theme	immunosorbent	1371:1383	arg1	ELISA					1392:1396	ELISA	1392:1396	ELISA	1392:1396	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	8	111	theme	immunosorbent	1371:1383	arg1	assay					1385:1389	an enzyme-linked immunosorbent assay	1354:1389	an enzyme-linked immunosorbent assay (ELISA) kit	1354:1401	The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit.
37311500	6	112	theme	Kit-8	1152:1156	arg1	kit					1166:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	2	113	theme	anti-inflammatory	456:472	arg1	effects					474:480	its anti-inflammatory effects	452:480	its anti-inflammatory effects	452:480	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	2	114	theme	essential	355:363	arg1	RCEO					370:373	RCEO	370:373	RCEO	370:373	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	2	114	theme	essential	355:363	arg1	oil					365:367	essential oil	355:367	Ramulus Cinnamomi essential oil (RCEO)	337:374	The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated.
37311500	11	115	dep	established	1634:1644	arg1	RESULTS					1623:1629	RESULTS	1623:1629	RESULTS	1623:1629	RESULTS We established a cell model for evaluating NAAA activity, and we found that RCEO inhibited the NAAA activity with an IC50 of 5.64 ± 0.62 μg/mL.
37311500	6	116	theme	Cell	1138:1141	arg1	kit					1166:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	a Cell Counting Kit-8 (CCK-8) kit	1136:1168	The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit.
37311500	16	117	with	interaction	2622:2632	arg1	LEU152					2692:2697	LEU152	2692:2697	LEU152 of human NAAA	2692:2711	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	16	117	with	interaction	2622:2632	arg1	TRP181					2643:2648	the TRP181	2639:2648	the TRP181	2639:2648	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	16	118	theme	catalytic	2566:2574	arg1	cavity					2576:2581	the catalytic cavity	2562:2581	the catalytic cavity of NAAA	2562:2589	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	3	119	theme	STUDY	525:529	arg1	AIM					514:516	AIM	514:516	AIM OF THE STUDY	514:529	AIM OF THE STUDY To investigate whether N-acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO.
37311500	16	120	with	interactions	2674:2685	arg1	LEU152					2692:2697	LEU152	2692:2697	LEU152 of human NAAA	2692:2711	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	16	120	with	interactions	2674:2685	arg1	TRP181					2643:2648	the TRP181	2639:2648	the TRP181	2639:2648	Meanwhile, docking assays revealed that (E)-cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA.
37311500	7	121	theme	nitric	1175:1180	arg1	NO					1189:1190	NO	1189:1190	NO	1189:1190	The nitric oxide (NO) in the cell supernatant was measured using the Griess method.
37311500	7	121	theme	nitric	1175:1180	arg1	oxide					1182:1186	The nitric oxide	1171:1186	The nitric oxide (NO) in the cell supernatant	1171:1215	The nitric oxide (NO) in the cell supernatant was measured using the Griess method.
37311500	1	122	theme	Chinese	255:261	arg1	TCM					273:275	TCM	273:275	TCM	273:275	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	122	theme	Chinese	255:261	arg1	RELEVANCE					158:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE	137:166	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
37311500	1	122	theme	Chinese	255:261	arg1	medicine					263:270	a traditional Chinese medicine	241:270	a traditional Chinese medicine (TCM) with anti-inflammatory effects	241:307	ETHNOPHARMACOLOGICAL RELEVANCE Ramulus Cinnamomi, the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects.
36034903	6	0	theme	functional	1257:1266	arg1	foods					1268:1272	functional foods	1257:1272	functional foods	1257:1272	The findings indicated that PPSL10 could be developed as immune-modulator in the field of functional foods.
36034903	1	1	dep	polysaccharides	303:317	arg1	PPSL100					320:326	PPSL100	320:326	PPSL100	320:326	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	1	1	dep	polysaccharides	303:317	arg1	polysaccharides					303:317	three graded polysaccharides	290:317	three graded polysaccharides (PPSL100, PPSL10, and PPSL1)	290:346	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	1	1	dep	polysaccharides	303:317	arg1	PPSL10					329:334	PPSL10	329:334	PPSL10	329:334	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	1	1	dep	polysaccharides	303:317	arg1	PPSL1					341:345	PPSL1	341:345	PPSL1	341:345	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	5	2	theme	weight	1150:1155	arg1	contents					1098:1105	the high contents	1089:1105	the high contents of above compositions and medium molecular weight (32 kDa)	1089:1164	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	1	3	from	membranes	384:392	arg1	sequence					419:426	sequence	419:426	sequence	419:426	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	0	4	from	properties	16:25	arg1	peels					92:96	the peels	88:96	the peels of stem lettuce (Lactuca sativa)	88:129	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	4	5	theme	acid	814:817	arg1	contents					766:773	the highest contents	754:773	the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%)	754:866	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	5	6	theme	high	1093:1096	arg1	contents					1098:1105	the high contents	1089:1105	the high contents of above compositions and medium molecular weight (32 kDa)	1089:1164	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	0	7	theme	lettuce	106:112	arg1	peels					92:96	the peels	88:96	the peels of stem lettuce (Lactuca sativa)	88:129	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	0	8	dep	Lactuca	115:121	arg1	sativa					123:128	Lactuca sativa	115:128	Lactuca sativa	115:128	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	0	9	theme	stem	101:104	arg1	lettuce					106:112	stem lettuce	101:112	stem lettuce (Lactuca sativa)	101:129	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	0	9	theme	stem	101:104	arg1	Lactuca					115:121	Lactuca	115:121	Lactuca	115:121	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	4	10	theme	2.55	820:823	arg1	±					825:825	±	825:825	±	825:825	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	1	11	theme	stem	264:267	arg1	PPSLs					278:282	stem lettuce (PPSLs)	264:283	stem lettuce (PPSLs)	264:283	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	2	12	theme	immune-modulatory	464:480	arg1	activity					482:489	immune-modulatory activity	464:489	immune-modulatory activity	464:489	The physicochemical properties and immune-modulatory activity of three PPSLs fractions were analyzed and compared.
36034903	4	13	theme	highest	758:764	arg1	contents					766:773	the highest contents	754:773	the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%)	754:866	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	4	14	theme	0.20	861:864	arg1	±					859:859	±	859:859	±	859:859	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	3	15	theme	polysaccharides	623:637	arg1	peak					615:618	characteristic absorption peak	589:618	characteristic absorption peak of polysaccharides determined by FT-IR	589:657	Results showed that all three fractions have characteristic absorption peak of polysaccharides determined by FT-IR, and their monosaccharide composition only consisted of glucose determined by HPLC.
36034903	0	16	from	activity	48:55	arg1	peels					92:96	the peels	88:96	the peels of stem lettuce (Lactuca sativa)	88:129	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	4	17	theme	uronic	807:812	arg1	%					831:831	2.55 ± 0.10%	820:831	2.55 ± 0.10%	820:831	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	4	17	theme	uronic	807:812	arg1	acid					814:817	uronic acid	807:817	uronic acid (2.55 ± 0.10%)	807:832	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	1	18	theme	kDa	412:414	arg1	membranes					384:392	ultrafiltration membranes	368:392	ultrafiltration membranes of 100, 10, and 1 kDa in sequence	368:426	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	4	19	theme	0.10	827:830	arg1	±					825:825	±	825:825	±	825:825	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	0	20	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	1	21	theme	lettuce	269:275	arg1	PPSLs					278:282	stem lettuce (PPSLs)	264:283	stem lettuce (PPSLs)	264:283	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	5	22	theme	macrophages	951:961	arg1	model					963:967	RAW264.7 macrophages model	942:967	RAW264.7 macrophages model	942:967	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	1	23	theme	cascade	178:184	arg1	technology					195:204	cascade membrane technology	178:204	cascade membrane technology	178:204	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	0	24	theme	immune-enhancing	31:46	arg1	activity					48:55	immune-enhancing activity	31:55	immune-enhancing activity	31:55	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	4	25	theme	sulfate	839:845	arg1	%					865:865	4.15 ± 0.20%	854:865	4.15 ± 0.20%	854:865	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	4	25	theme	sulfate	839:845	arg1	group					847:851	sulfate group	839:851	sulfate group (4.15 ± 0.20%)	839:866	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	5	26	theme	RAW264.7	942:949	arg1	macrophages					951:961	RAW264.7 macrophages	942:961	RAW264.7 macrophages model	942:967	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	1	27	theme	membrane	186:193	arg1	technology					195:204	cascade membrane technology	178:204	cascade membrane technology	178:204	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	4	28	theme	total	778:782	arg1	%					803:803	88.09 ± 3.52%	791:803	88.09 ± 3.52%	791:803	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	4	28	theme	total	778:782	arg1	sugar					784:788	total sugar	778:788	total sugar (88.09 ± 3.52%)	778:804	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	1	29	theme	PPSLs	278:282	arg1	peels					255:259	the peels	251:259	the peels of stem lettuce (PPSLs)	251:283	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	2	30	theme	fractions	506:514	arg1	properties					449:458	The physicochemical properties	429:458	The physicochemical properties	429:458	The physicochemical properties and immune-modulatory activity of three PPSLs fractions were analyzed and compared.
36034903	2	30	theme	fractions	506:514	arg1	activity					482:489	immune-modulatory activity	464:489	immune-modulatory activity	464:489	The physicochemical properties and immune-modulatory activity of three PPSLs fractions were analyzed and compared.
36034903	4	31	theme	group	847:851	arg1	contents					766:773	the highest contents	754:773	the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%)	754:866	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	0	32	theme	cascade	134:140	arg1	technology					151:160	cascade membrane technology	134:160	cascade membrane technology	134:160	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	0	33	from	peels	92:96	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	0	33	from	peels	92:96	arg1	activity					48:55	immune-enhancing activity	31:55	immune-enhancing activity	31:55	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	0	33	from	peels	92:96	arg1	polysaccharides					67:81	graded polysaccharides	60:81	graded polysaccharides from the peels of stem lettuce (Lactuca sativa)	60:129	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	5	34	theme	immune-enhancing	908:923	arg1	activities					925:934	immune-enhancing activities	908:934	immune-enhancing activities using RAW264.7 macrophages model	908:967	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	5	35	theme	nitric	1042:1047	arg1	oxide					1049:1053	nitric oxide	1042:1053	nitric oxide generation	1042:1064	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	1	36	from	peels	255:259	arg1	polysaccharides					230:244	polysaccharides	230:244	polysaccharides from the peels of stem lettuce (PPSLs)	230:283	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	3	37	theme	absorption	604:613	arg1	peak					615:618	characteristic absorption peak	589:618	characteristic absorption peak of polysaccharides determined by FT-IR	589:657	Results showed that all three fractions have characteristic absorption peak of polysaccharides determined by FT-IR, and their monosaccharide composition only consisted of glucose determined by HPLC.
36034903	4	38	contain	had	750:752	arg2	contents					766:773	the highest contents	754:773	the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%)	754:866	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	4	38	contain	had	750:752	arg1	PPSL10					743:748	PPSL10	743:748	PPSL10	743:748	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	0	39	theme	polysaccharides	67:81	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	0	39	theme	polysaccharides	67:81	arg1	activity					48:55	immune-enhancing activity	31:55	immune-enhancing activity	31:55	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	3	40	dep	consisted	702:710	arg1	determined					723:732	determined	723:732	determined by HPLC	723:740	Results showed that all three fractions have characteristic absorption peak of polysaccharides determined by FT-IR, and their monosaccharide composition only consisted of glucose determined by HPLC.
36034903	6	41	theme	foods	1268:1272	arg1	field					1248:1252	the field	1244:1252	the field of functional foods	1244:1272	The findings indicated that PPSL10 could be developed as immune-modulator in the field of functional foods.
36034903	5	42	theme	medium	1133:1138	arg1	weight					1150:1155	medium molecular weight	1133:1155	medium molecular weight (32 kDa)	1133:1164	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	5	42	theme	medium	1133:1138	arg1	kDa					1161:1163	32 kDa	1158:1163	32 kDa	1158:1163	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	5	43	theme	red	1034:1036	arg1	phagocytosis					1010:1021	phagocytosis	1010:1021	phagocytosis	1010:1021	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	0	44	theme	graded	60:65	arg1	polysaccharides					67:81	graded polysaccharides	60:81	graded polysaccharides from the peels of stem lettuce (Lactuca sativa)	60:129	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	5	45	theme	generation	1055:1064	arg1	phagocytosis					1010:1021	phagocytosis	1010:1021	phagocytosis	1010:1021	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	0	46	theme	membrane	142:149	arg1	technology					151:160	cascade membrane technology	134:160	cascade membrane technology	134:160	Physicochemical properties and immune-enhancing activity of graded polysaccharides from the peels of stem lettuce (Lactuca sativa) by cascade membrane technology.
36034903	1	47	theme	ultrafiltration	368:382	arg1	membranes					384:392	ultrafiltration membranes	368:392	ultrafiltration membranes of 100, 10, and 1 kDa in sequence	368:426	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	4	48	theme	±	797:797	arg1	%					803:803	88.09 ± 3.52%	791:803	88.09 ± 3.52%	791:803	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	4	48	theme	±	797:797	arg1	sugar					784:788	total sugar	778:788	total sugar (88.09 ± 3.52%)	778:804	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	1	49	theme	graded	296:301	arg1	PPSL100					320:326	PPSL100	320:326	PPSL100	320:326	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	1	49	theme	graded	296:301	arg1	polysaccharides					303:317	three graded polysaccharides	290:317	three graded polysaccharides (PPSL100, PPSL10, and PPSL1)	290:346	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	1	49	theme	graded	296:301	arg1	PPSL10					329:334	PPSL10	329:334	PPSL10	329:334	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	1	49	theme	graded	296:301	arg1	PPSL1					341:345	PPSL1	341:345	PPSL1	341:345	In this study, cascade membrane technology was applied to classify polysaccharides from the peels of stem lettuce (PPSLs), and three graded polysaccharides (PPSL100, PPSL10, and PPSL1) were obtained using ultrafiltration membranes of 100, 10, and 1 kDa in sequence.
36034903	5	50	theme	above	1110:1114	arg1	compositions					1116:1127	above compositions	1110:1127	above compositions	1110:1127	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	3	51	theme	characteristic	589:602	arg1	peak					615:618	characteristic absorption peak	589:618	characteristic absorption peak of polysaccharides determined by FT-IR	589:657	Results showed that all three fractions have characteristic absorption peak of polysaccharides determined by FT-IR, and their monosaccharide composition only consisted of glucose determined by HPLC.
36034903	4	52	theme	4.15	854:857	arg1	±					859:859	±	859:859	±	859:859	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	5	53	theme	compositions	1116:1127	arg1	contents					1098:1105	the high contents	1089:1105	the high contents of above compositions and medium molecular weight (32 kDa)	1089:1164	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	5	54	theme	oxide	1049:1053	arg1	generation					1055:1064	nitric oxide generation	1042:1064	nitric oxide generation	1042:1064	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	4	55	theme	sugar	784:788	arg1	contents					766:773	the highest contents	754:773	the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%)	754:866	PPSL10 had the highest contents of total sugar (88.09 ± 3.52%), uronic acid (2.55 ± 0.10%), and sulfate group (4.15 ± 0.20%).
36034903	2	56	theme	physicochemical	433:447	arg1	properties					449:458	The physicochemical properties	429:458	The physicochemical properties	429:458	The physicochemical properties and immune-modulatory activity of three PPSLs fractions were analyzed and compared.
36034903	3	57	theme	monosaccharide	670:683	arg1	composition					685:695	their monosaccharide composition	664:695	their monosaccharide composition	664:695	Results showed that all three fractions have characteristic absorption peak of polysaccharides determined by FT-IR, and their monosaccharide composition only consisted of glucose determined by HPLC.
36034903	3	58	contain	have	584:587	arg1	fractions					574:582	all three fractions	564:582	all three fractions	564:582	Results showed that all three fractions have characteristic absorption peak of polysaccharides determined by FT-IR, and their monosaccharide composition only consisted of glucose determined by HPLC.
36034903	3	58	contain	have	584:587	arg2	peak					615:618	characteristic absorption peak	589:618	characteristic absorption peak of polysaccharides determined by FT-IR	589:657	Results showed that all three fractions have characteristic absorption peak of polysaccharides determined by FT-IR, and their monosaccharide composition only consisted of glucose determined by HPLC.
36034903	5	59	theme	molecular	1140:1148	arg1	weight					1150:1155	medium molecular weight	1133:1155	medium molecular weight (32 kDa)	1133:1164	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	5	59	theme	molecular	1140:1148	arg1	kDa					1161:1163	32 kDa	1158:1163	32 kDa	1158:1163	Besides, all three fractions exhibited immune-enhancing activities using RAW264.7 macrophages model, and PPSL10 was the best able to promote phagocytosis of neutral red and nitric oxide generation, which might relate to the high contents of above compositions and medium molecular weight (32 kDa).
36034903	2	60	theme	PPSLs	500:504	arg1	fractions					506:514	three PPSLs fractions	494:514	three PPSLs fractions	494:514	The physicochemical properties and immune-modulatory activity of three PPSLs fractions were analyzed and compared.
35707876	2	0	theme	inflammatory	495:506	arg1	disease					514:520	inflammatory bowel disease	495:520	inflammatory bowel disease induced by dextran sulfate solution	495:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	0	1	from	Lactobacillus	23:35	arg1	Exopolysaccharide					0:16	Exopolysaccharide	0:16	Exopolysaccharide from Lactobacillus rhamnosus ZFM231	0:52	Exopolysaccharide from Lactobacillus rhamnosus ZFM231 alleviates DSS-induced colitis in mice by regulating gut microbiota.
35707876	4	2	theme	model	767:771	arg1	group					773:777	the model group	763:777	the model group	763:777	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	2	3	theme	present	371:377	arg1	study					379:383	the present study	367:383	the present study	367:383	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	4	4	theme	fatty	1020:1024	arg1	acid					1050:1053	acetic acid	1043:1053	acetic acid	1043:1053	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	4	theme	fatty	1020:1024	arg1	acid					1066:1069	propionic acid	1056:1069	propionic acid	1056:1069	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	4	theme	fatty	1020:1024	arg1	acid					1083:1086	butyric acid	1075:1086	butyric acid	1075:1086	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	4	theme	fatty	1020:1024	arg1	acids					1026:1030	short-chain fatty acids	1008:1030	short-chain fatty acids	1008:1030	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	1	5	theme	homeostasis	351:361	arg1	effect					306:311	antitumor effect	296:311	antitumor effect	296:311	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	5	theme	homeostasis	351:361	arg1	regulation					318:327	regulation	318:327	regulation of intestinal microbe homeostasis	318:361	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	5	theme	homeostasis	351:361	arg1	regulation					260:269	immune regulation	253:269	immune regulation	253:269	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	5	theme	homeostasis	351:361	arg1	properties					284:293	antioxidant properties	272:293	antioxidant properties	272:293	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	4	6	theme	EPS-treated	1362:1372	arg1	mice					1374:1377	EPS-treated mice	1362:1377	EPS-treated mice	1362:1377	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	7	theme	pro-inflammatory	1266:1281	arg1	factor-α					1305:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	8	theme	necrosis	1296:1303	arg1	factor-α					1305:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	2	9	theme	sulfate	541:547	arg1	solution					549:556	dextran sulfate solution	533:556	dextran sulfate solution	533:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	3	10	theme	disease	640:646	arg1	score					663:667	the disease activity index score	636:667	the disease activity index score	636:667	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	3	11	theme	weight	607:612	arg1	loss					614:617	weight loss	607:617	weight loss	607:617	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	1	12	theme	antioxidant	272:282	arg1	properties					284:293	antioxidant properties	272:293	antioxidant properties	272:293	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	0	13	theme	gut	107:109	arg1	microbiota					111:120	gut microbiota	107:120	gut microbiota	107:120	Exopolysaccharide from Lactobacillus rhamnosus ZFM231 alleviates DSS-induced colitis in mice by regulating gut microbiota.
35707876	0	14	from	colitis	77:83	arg1	mice					88:91	mice	88:91	mice	88:91	Exopolysaccharide from Lactobacillus rhamnosus ZFM231 alleviates DSS-induced colitis in mice by regulating gut microbiota.
35707876	4	15	theme	phylum	869:874	arg1	levels					886:891	both phylum and genus levels	864:891	levels	886:891	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	16	from	modulation	971:980	arg1	microbiota					989:998	gut microbiota	985:998	gut microbiota	985:998	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	3	17	from	length	628:633	arg1	mice					698:701	mice	698:701	mice	698:701	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	3	18	from	score	663:667	arg1	mice					698:701	mice	698:701	mice	698:701	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	5	19	theme	L.	1424:1425	arg1	ZFM231					1437:1442	L. rhamnosus ZFM231	1424:1442	L. rhamnosus ZFM231	1424:1442	CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti-ulcerative colitis.
35707876	4	20	theme	colonic	1345:1351	arg1	cells					1353:1357	the colonic cells	1341:1357	the colonic cells of EPS-treated mice	1341:1377	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	6	21	theme	new	1566:1568	arg1	insights					1570:1577	new insights	1566:1577	new insights into the probiotic effect of EPS	1566:1610	The experimental results provide new insights into the probiotic effect of EPS.
35707876	2	22	theme	intestinal	465:474	arg1	flora					476:480	the intestinal flora	461:480	the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution	461:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	4	23	theme	composition	831:841	arg1	diversity					810:818	the diversity	806:818	the diversity of and the composition of gut microbiota at both phylum and genus levels	806:891	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	24	theme	factor	1189:1194	arg1	factor-β					1216:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	25	theme	microbial	1111:1119	arg1	metabolism					1121:1130	intestinal microbial metabolism	1100:1130	intestinal microbial metabolism	1100:1130	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	1	26	theme	other	193:197	arg1	probiotics					199:208	other probiotics	193:208	other probiotics	193:208	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	3	27	theme	EPS	734:736	arg1	treatment					738:746	EPS treatment	734:746	EPS treatment	734:746	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	4	28	theme	microbiota	850:859	arg1	composition					831:841	and the composition	823:841	composition	831:841	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	29	theme	anti-inflammatory	1171:1187	arg1	factor-β					1216:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	3	30	from	damage	688:693	arg1	mice					698:701	mice	698:701	mice	698:701	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	4	31	theme	intestinal	1100:1109	arg1	metabolism					1121:1130	intestinal microbial metabolism	1100:1130	intestinal microbial metabolism	1100:1130	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	2	32	theme	modulatory	390:399	arg1	effect					401:406	the modulatory effect	386:406	the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution	386:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	4	33	theme	EPS-treated	787:797	arg1	group					799:803	the EPS-treated group	783:803	the EPS-treated group	783:803	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	34	theme	gut	846:848	arg1	microbiota					850:859	gut microbiota	846:859	gut microbiota	846:859	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	35	theme	growth	1209:1214	arg1	factor-β					1216:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	5	36	dep	CONCLUSION	1380:1389	arg1	clear					1397:1401	clear	1397:1401	clear	1397:1401	CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti-ulcerative colitis.
35707876	4	37	theme	short-chain	1008:1018	arg1	acid					1050:1053	acetic acid	1043:1053	acetic acid	1043:1053	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	37	theme	short-chain	1008:1018	arg1	acid					1066:1069	propionic acid	1056:1069	propionic acid	1056:1069	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	37	theme	short-chain	1008:1018	arg1	acid					1083:1086	butyric acid	1075:1086	butyric acid	1075:1086	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	37	theme	short-chain	1008:1018	arg1	acids					1026:1030	short-chain fatty acids	1008:1030	short-chain fatty acids	1008:1030	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	1	38	theme	antitumor	296:304	arg1	effect					306:311	antitumor effect	296:311	antitumor effect	296:311	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	4	39	theme	normal	932:937	arg1	group					939:943	normal group	932:943	normal group	932:943	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	40	theme	transforning	1196:1207	arg1	factor-β					1216:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	anti-inflammatory factor transforning growth factor-β	1171:1223	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	3	41	theme	RESULTS	576:582	arg1	Results					584:590	RESULTS Results	576:590	RESULTS Results	576:590	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	3	42	from	loss	614:617	arg1	mice					698:701	mice	698:701	mice	698:701	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	4	43	theme	effective	961:969	arg1	modulation					971:980	the effective modulation	957:980	the effective modulation on gut microbiota by EPS	957:1005	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	44	theme	tumor	1290:1294	arg1	factor-α					1305:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	45	theme	butyric	1075:1081	arg1	acid					1083:1086	butyric acid	1075:1086	butyric acid	1075:1086	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	6	46	theme	experimental	1537:1548	arg1	results					1550:1556	The experimental results	1533:1556	The experimental results	1533:1556	The experimental results provide new insights into the probiotic effect of EPS.
35707876	2	47	theme	mice	485:488	arg1	flora					476:480	the intestinal flora	461:480	the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution	461:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	4	48	theme	factor	1283:1288	arg1	factor-α					1305:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	pro-inflammatory factor tumor necrosis factor-α	1266:1312	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	49	located	found	898:902	arg1	group					799:803	the EPS-treated group	783:803	the EPS-treated group	783:803	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	49	located	found	898:902	arg2	diversity					810:818	the diversity	806:818	the diversity of and the composition of gut microbiota at both phylum and genus levels	806:891	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	1	50	theme	many	230:233	arg1	effect					306:311	antitumor effect	296:311	antitumor effect	296:311	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	50	theme	many	230:233	arg1	regulation					318:327	regulation	318:327	regulation of intestinal microbe homeostasis	318:361	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	50	theme	many	230:233	arg1	properties					284:293	antioxidant properties	272:293	antioxidant properties	272:293	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	50	theme	many	230:233	arg1	regulation					260:269	immune regulation	253:269	immune regulation	253:269	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	50	theme	many	230:233	arg1	benefits					235:242	many benefits	230:242	many benefits	230:242	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	4	51	theme	gut	985:987	arg1	microbiota					989:998	gut microbiota	985:998	gut microbiota	985:998	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	3	52	theme	activity	648:655	arg1	score					663:667	the disease activity index score	636:667	the disease activity index score	636:667	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	3	53	theme	colonic	620:626	arg1	length					628:633	colonic length	620:633	colonic length	620:633	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	2	54	theme	EPS	411:413	arg1	effect					401:406	the modulatory effect	386:406	the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution	386:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	4	55	theme	mice	1374:1377	arg1	cells					1353:1357	the colonic cells	1341:1357	the colonic cells of EPS-treated mice	1341:1377	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	5	56	theme	colitis	1524:1530	arg1	property					1496:1503	the property	1492:1503	the property of anti-ulcerative colitis	1492:1530	CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti-ulcerative colitis.
35707876	3	57	theme	index	657:661	arg1	score					663:667	the disease activity index score	636:667	the disease activity index score	636:667	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	2	58	dep	Lactobacillus	427:439	arg1	rhamnosus					441:449	Lactobacillus rhamnosus ZFM231	427:456	Lactobacillus rhamnosus ZFM231	427:456	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	2	58	dep	Lactobacillus	427:439	arg1	ZFM231					451:456	ZFM231	451:456	ZFM231	451:456	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	0	59	theme	DSS-induced	65:75	arg1	colitis					77:83	DSS-induced colitis	65:83	DSS-induced colitis in mice	65:91	Exopolysaccharide from Lactobacillus rhamnosus ZFM231 alleviates DSS-induced colitis in mice by regulating gut microbiota.
35707876	4	60	theme	genus	880:884	arg1	levels					886:891	both phylum and genus levels	864:891	levels	886:891	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	5	61	theme	functional	1470:1479	arg1	foods					1481:1485	functional foods	1470:1485	functional foods	1470:1485	CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti-ulcerative colitis.
35707876	6	62	theme	EPS	1608:1610	arg1	effect					1598:1603	the probiotic effect	1584:1603	the probiotic effect of EPS	1584:1610	The experimental results provide new insights into the probiotic effect of EPS.
35707876	4	63	theme	factor-α	1305:1312	arg1	level					1257:1261	the level	1253:1261	the level of pro-inflammatory factor tumor necrosis factor-α	1253:1312	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	64	theme	group	939:943	arg1	levels					922:927	the levels	918:927	the levels of normal group	918:943	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	1	65	theme	intestinal	332:341	arg1	homeostasis					351:361	intestinal microbe homeostasis	332:361	intestinal microbe homeostasis	332:361	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	2	66	from	effect	401:406	arg1	flora					476:480	the intestinal flora	461:480	the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution	461:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	4	67	theme	acetic	1043:1048	arg1	acid					1050:1053	acetic acid	1043:1053	acetic acid	1043:1053	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	5	68	theme	anti-ulcerative	1508:1522	arg1	colitis					1524:1530	anti-ulcerative colitis	1508:1530	anti-ulcerative colitis	1508:1530	CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti-ulcerative colitis.
35707876	3	69	theme	colonic	673:679	arg1	damage					688:693	colonic tissue damage	673:693	colonic tissue damage	673:693	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	0	70	dep	Lactobacillus	23:35	arg1	ZFM231					47:52	Lactobacillus rhamnosus ZFM231	23:52	Lactobacillus rhamnosus ZFM231	23:52	Exopolysaccharide from Lactobacillus rhamnosus ZFM231 alleviates DSS-induced colitis in mice by regulating gut microbiota.
35707876	7	71	theme	Chemical	1631:1638	arg1	Industry					1640:1647	Chemical Industry	1631:1647	Chemical Industry	1631:1647	© 2022 Society of Chemical Industry.
35707876	5	72	from	application	1455:1465	arg1	foods					1481:1485	functional foods	1470:1485	functional foods	1470:1485	CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti-ulcerative colitis.
35707876	4	73	theme	propionic	1056:1064	arg1	acid					1066:1069	propionic acid	1056:1069	propionic acid	1056:1069	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	4	74	from	levels	886:891	arg1	diversity					810:818	the diversity	806:818	the diversity of and the composition of gut microbiota at both phylum and genus levels	806:891	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	3	75	theme	tissue	681:686	arg1	damage					688:693	colonic tissue damage	673:693	colonic tissue damage	673:693	RESULTS Results indicated that weight loss, colonic length, the disease activity index score and colonic tissue damage in mice were significantly improved by EPS treatment.
35707876	5	76	theme	rhamnosus	1427:1435	arg1	ZFM231					1437:1442	L. rhamnosus ZFM231	1424:1442	L. rhamnosus ZFM231	1424:1442	CONCLUSION It is clear that EPS produced by L. rhamnosus ZFM231 could find application in functional foods with the property of anti-ulcerative colitis.
35707876	6	77	theme	probiotic	1588:1596	arg1	effect					1598:1603	the probiotic effect	1584:1603	the probiotic effect of EPS	1584:1610	The experimental results provide new insights into the probiotic effect of EPS.
35707876	4	78	theme	factor-β	1216:1223	arg1	level					1162:1166	the level	1158:1166	the level of anti-inflammatory factor transforning growth factor-β	1158:1223	Compared with the model group, in the EPS-treated group, the diversity of and the composition of gut microbiota at both phylum and genus levels were found to recover to the levels of normal group, indicating the effective modulation on gut microbiota by EPS; short-chain fatty acids, including acetic acid, propionic acid and butyric acid produced by intestinal microbial metabolism, increased significantly; the level of anti-inflammatory factor transforning growth factor-β significantly increased and the level of pro-inflammatory factor tumor necrosis factor-α significantly decreased in the colonic cells of EPS-treated mice.
35707876	2	79	theme	bowel	508:512	arg1	disease					514:520	inflammatory bowel disease	495:520	inflammatory bowel disease induced by dextran sulfate solution	495:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	1	80	theme	microbe	343:349	arg1	homeostasis					351:361	intestinal microbe homeostasis	332:361	intestinal microbe homeostasis	332:361	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	1	81	theme	immune	253:258	arg1	regulation					260:269	immune regulation	253:269	immune regulation	253:269	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	2	82	with	mice	485:488	arg1	disease					514:520	inflammatory bowel disease	495:520	inflammatory bowel disease induced by dextran sulfate solution	495:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35707876	1	83	dep	BACKGROUND	123:132	arg1	associated					214:223	associated	214:223	are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis	210:361	BACKGROUND The exopolysaccharides (EPS) produced by Lactobacillus and other probiotics are associated with many benefits, such as immune regulation, antioxidant properties, antitumor effect, and regulation of intestinal microbe homeostasis.
35707876	2	84	theme	dextran	533:539	arg1	sulfate					541:547	dextran sulfate	533:547	dextran sulfate solution	533:556	In the present study, the modulatory effect of EPS produced by Lactobacillus rhamnosus ZFM231 on the intestinal flora of mice with inflammatory bowel disease induced by dextran sulfate solution was investigated.
35254234	7	0	theme	fatty	746:750	arg1	acids					752:756	The major fatty acids	736:756	The major fatty acids	736:756	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	0	theme	fatty	746:750	arg1	 0					776:777	 0	776:777	 0	776:777	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	0	theme	fatty	746:750	arg1	anteiso-C15 					763:774	anteiso-C15 	763:774	anteiso-C15 	763:774	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	3	1	theme	aerobic	208:214	arg1	bacterium					271:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	3	1	theme	aerobic	208:214	arg1	BG109T					195:200	BG109T	195:200	BG109T	195:200	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	4	2	dep	showed	289:294	arg1	optimum					407:413	optimum	407:413	optimum	407:413	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	5	3	theme	diagnostic	563:572	arg1	amounts					520:526	major amounts	514:526	major amounts of meso-diaminopimelic acid	514:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	3	theme	diagnostic	563:572	arg1	acid					582:585	the diagnostic diamino acid	559:585	the diagnostic diamino acid	559:585	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	3	theme	diagnostic	563:572	arg1	acid					551:554	meso-diaminopimelic acid	531:554	meso-diaminopimelic acid	531:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	9	4	theme	JSM	1169:1171	arg1	100118T					1173:1179	Metabacillus crassostreae JSM 100118T	1143:1179	Metabacillus crassostreae JSM 100118T (97.13 %)	1143:1189	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	4	theme	JSM	1169:1171	arg1	%					1188:1188	97.13 %	1182:1188	97.13 %	1182:1188	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	5	theme	Metabacillus	1050:1061	arg1	DSL-17T					1079:1085	Metabacillus sediminilitoris DSL-17T	1050:1085	Metabacillus sediminilitoris DSL-17T (98.17 %)	1050:1095	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	5	theme	Metabacillus	1050:1061	arg1	%					1094:1094	98.17 %	1088:1094	98.17 %	1088:1094	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	5	6	theme	diamino	574:580	arg1	amounts					520:526	major amounts	514:526	major amounts of meso-diaminopimelic acid	514:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	6	theme	diamino	574:580	arg1	acid					582:585	the diagnostic diamino acid	559:585	the diagnostic diamino acid	559:585	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	6	theme	diamino	574:580	arg1	acid					551:554	meso-diaminopimelic acid	531:554	meso-diaminopimelic acid	531:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	10	7	theme	level	1284:1288	arg1	relatedness					1290:1300	The genome level relatedness	1273:1300	The genome level relatedness	1273:1300	The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus.
35254234	9	8	theme	highest	960:966	arg1	similarities					991:1002	highest 16S rRNA gene sequence similarities	960:1002	highest 16S rRNA gene sequence similarities	960:1002	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	2	9	theme	polyphasic	156:165	arg1	characterization					177:192	polyphasic taxonomic characterization	156:192	polyphasic taxonomic characterization	156:192	A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization.
35254234	7	10	dep	anteiso-C15 	763:774	arg1	acids					752:756	The major fatty acids	736:756	The major fatty acids	736:756	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	10	dep	anteiso-C15 	763:774	arg1	 0					776:777	 0	776:777	 0	776:777	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	10	dep	anteiso-C15 	763:774	arg1	anteiso-C15 					763:774	anteiso-C15 	763:774	anteiso-C15 	763:774	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	5	11	theme	major	514:518	arg1	acid					582:585	the diagnostic diamino acid	559:585	the diagnostic diamino acid	559:585	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	11	theme	major	514:518	arg1	amounts					520:526	major amounts	514:526	major amounts of meso-diaminopimelic acid	514:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	11	theme	major	514:518	arg1	acid					551:554	meso-diaminopimelic acid	531:554	meso-diaminopimelic acid	531:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	12	theme	BG109T	455:460	arg1	MK-7					466:469	MK-7	466:469	MK-7	466:469	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	12	theme	BG109T	455:460	arg1	menaquinone					440:450	The predominant menaquinone	424:450	The predominant menaquinone of BG109T	424:460	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	11	13	theme	name	1512:1515	arg1	sp					1539:1540	the name Metabacillus bambusae sp	1508:1540	the name Metabacillus bambusae sp	1508:1540	Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus, for which the name Metabacillus bambusae sp.
35254234	8	14	theme	Metabacillus	932:943	arg1	members					911:917	members	911:917	members of the genus Metabacillus	911:943	The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus.
35254234	1	15	theme	grove	53:57	arg1	soil					59:62	bamboo grove soil	46:62	bamboo grove soil	46:62	nov., isolated from bamboo grove soil.
35254234	9	16	theme	rRNA	972:975	arg1	similarities					991:1002	highest 16S rRNA gene sequence similarities	960:1002	highest 16S rRNA gene sequence similarities	960:1002	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	11	17	theme	BG109T	1413:1418	arg1	merits					1420:1425	BG109T merits	1413:1425	BG109T merits	1413:1425	Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus, for which the name Metabacillus bambusae sp.
35254234	9	18	theme	Metabacillus	1010:1021	arg1	%					1046:1046	99.26 %	1040:1046	99.26 %	1040:1046	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	18	theme	Metabacillus	1010:1021	arg1	elymi					1023:1027	'Metabacillus elymi' KUDC1714	1009:1037	'Metabacillus elymi' KUDC1714 (99.26 %)	1009:1047	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	11	19	theme	Metabacillus	1517:1528	arg1	sp					1539:1540	the name Metabacillus bambusae sp	1508:1540	the name Metabacillus bambusae sp	1508:1540	Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus, for which the name Metabacillus bambusae sp.
35254234	3	20	theme	Gram-stain-positive	229:247	arg1	bacterium					271:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	3	20	theme	Gram-stain-positive	229:247	arg1	BG109T					195:200	BG109T	195:200	BG109T	195:200	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	9	21	theme	gene	977:980	arg1	similarities					991:1002	highest 16S rRNA gene sequence similarities	960:1002	highest 16S rRNA gene sequence similarities	960:1002	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	2	22	theme	grove	127:131	arg1	soil					133:136	bamboo grove soil	120:136	bamboo grove soil	120:136	A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization.
35254234	5	23	theme	acid	551:554	arg1	acid					582:585	the diagnostic diamino acid	559:585	the diagnostic diamino acid	559:585	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	23	theme	acid	551:554	arg1	amounts					520:526	major amounts	514:526	major amounts of meso-diaminopimelic acid	514:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	23	theme	acid	551:554	arg1	acid					551:554	meso-diaminopimelic acid	531:554	meso-diaminopimelic acid	531:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	10	24	theme	genome	1277:1282	arg1	relatedness					1290:1300	The genome level relatedness	1273:1300	The genome level relatedness	1273:1300	The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus.
35254234	13	25	theme	=KCTC	1588:1592	arg1	BG109T					1580:1585	BG109T	1580:1585	BG109T (=KCTC 43190T=JCM 34515T)	1580:1611	The type strain is BG109T (=KCTC 43190T=JCM 34515T).
35254234	13	25	theme	=KCTC	1588:1592	arg1	34515T					1605:1610	=KCTC 43190T=JCM 34515T	1588:1610	=KCTC 43190T=JCM 34515T	1588:1610	The type strain is BG109T (=KCTC 43190T=JCM 34515T).
35254234	4	26	dep	optimum	316:322	arg1	37 °C					325:329	37 °C	325:329	37 °C	325:329	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	10	27	theme	other	1347:1351	arg1	species					1353:1359	other species	1347:1359	other species of the genus Metabacillus	1347:1385	The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus.
35254234	0	28	theme	bambusae	13:20	arg1	sp					22:23	Metabacillus bambusae sp	0:23	Metabacillus bambusae sp.	0:24	Metabacillus bambusae sp.
35254234	3	29	theme	non-motile	217:226	arg1	bacterium					271:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	3	29	theme	non-motile	217:226	arg1	BG109T					195:200	BG109T	195:200	BG109T	195:200	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	5	30	theme	cell	480:483	arg1	peptidoglycan					490:502	the cell wall peptidoglycan	476:502	the cell wall peptidoglycan	476:502	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	0	31	theme	Metabacillus	0:11	arg1	sp					22:23	Metabacillus bambusae sp	0:23	Metabacillus bambusae sp.	0:24	Metabacillus bambusae sp.
35254234	9	32	theme	Metabacillus	1143:1154	arg1	100118T					1173:1179	Metabacillus crassostreae JSM 100118T	1143:1179	Metabacillus crassostreae JSM 100118T (97.13 %)	1143:1189	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	32	theme	Metabacillus	1143:1154	arg1	%					1188:1188	97.13 %	1182:1188	97.13 %	1182:1188	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	5	33	theme	wall	485:488	arg1	peptidoglycan					490:502	the cell wall peptidoglycan	476:502	the cell wall peptidoglycan	476:502	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	34	theme	meso-diaminopimelic	531:549	arg1	acid					551:554	meso-diaminopimelic acid	531:554	meso-diaminopimelic acid	531:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	4	35	dep	pH	336:337	arg1	optimum					345:351	optimum	345:351	optimum	345:351	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	4	35	dep	pH	336:337	arg1	4-10					339:342	4-10	339:342	4-10	339:342	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	5	36	contain	contained	504:512	arg1	peptidoglycan					490:502	the cell wall peptidoglycan	476:502	the cell wall peptidoglycan	476:502	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	36	contain	contained	504:512	arg2	acid					582:585	the diagnostic diamino acid	559:585	the diagnostic diamino acid	559:585	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	36	contain	contained	504:512	arg2	acid					551:554	meso-diaminopimelic acid	531:554	meso-diaminopimelic acid	531:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	36	contain	contained	504:512	arg2	amounts					520:526	major amounts	514:526	major amounts of meso-diaminopimelic acid	514:554	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	13	37	theme	43190T=JCM	1594:1603	arg1	BG109T					1580:1585	BG109T	1580:1585	BG109T (=KCTC 43190T=JCM 34515T)	1580:1611	The type strain is BG109T (=KCTC 43190T=JCM 34515T).
35254234	13	37	theme	43190T=JCM	1594:1603	arg1	34515T					1605:1610	=KCTC 43190T=JCM 34515T	1588:1610	=KCTC 43190T=JCM 34515T	1588:1610	The type strain is BG109T (=KCTC 43190T=JCM 34515T).
35254234	9	38	theme	Metabacillus	1098:1109	arg1	SW-211T					1121:1127	Metabacillus litoralis SW-211T	1098:1127	Metabacillus litoralis SW-211T (98.16 %)	1098:1137	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	38	theme	Metabacillus	1098:1109	arg1	%					1136:1136	98.16 %	1130:1136	98.16 %	1130:1136	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	6	39	theme	polar	603:607	arg1	diphosphatidylglycerol					621:642	diphosphatidylglycerol	621:642	diphosphatidylglycerol	621:642	The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and unidentified phospholipids and glycolipids were also present.
35254234	6	39	theme	polar	603:607	arg1	lipids					609:614	The diagnostic polar lipids	588:614	The diagnostic polar lipids	588:614	The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and unidentified phospholipids and glycolipids were also present.
35254234	10	40	theme	BG109T	1335:1340	arg1	separation					1321:1330	the separation	1317:1330	the separation of BG109T from other species of the genus Metabacillus	1317:1385	The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus.
35254234	7	41	dep	 0	776:777	arg1	 0					815:816	 0	815:816	 0	815:816	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	41	dep	 0	776:777	arg1	 0					827:828	 0	827:828	 0	827:828	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	41	dep	 0	776:777	arg1	 0					789:790	 0	789:790	 0	789:790	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	41	dep	 0	776:777	arg1	 0					802:803	 0	802:803	 0	802:803	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	41	dep	 0	776:777	arg1	C16 					822:825	C16 	822:825	C16 	822:825	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	4	42	dep	optimum	407:413	arg1	%					420:420	0-1 %	416:420	0-1 %	416:420	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	4	43	theme	%	385:385	arg1	concentration					392:404	0-7 % NaCl concentration	381:404	0-7 % NaCl concentration	381:404	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	6	44	theme	diagnostic	592:601	arg1	diphosphatidylglycerol					621:642	diphosphatidylglycerol	621:642	diphosphatidylglycerol	621:642	The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and unidentified phospholipids and glycolipids were also present.
35254234	6	44	theme	diagnostic	592:601	arg1	lipids					609:614	The diagnostic polar lipids	588:614	The diagnostic polar lipids	588:614	The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and unidentified phospholipids and glycolipids were also present.
35254234	13	45	theme	type	1565:1568	arg1	BG109T					1580:1585	BG109T	1580:1585	BG109T (=KCTC 43190T=JCM 34515T)	1580:1611	The type strain is BG109T (=KCTC 43190T=JCM 34515T).
35254234	13	45	theme	type	1565:1568	arg1	strain					1570:1575	The type strain	1561:1575	The type strain	1561:1575	The type strain is BG109T (=KCTC 43190T=JCM 34515T).
35254234	3	46	theme	endospore-forming	253:269	arg1	bacterium					271:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium	205:279	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	3	46	theme	endospore-forming	253:269	arg1	BG109T					195:200	BG109T	195:200	BG109T	195:200	BG109T is an aerobic, non-motile, Gram-stain-positive and endospore-forming bacterium.
35254234	4	47	theme	0-7 	381:384	arg1	%					385:385	%	385:385	%	385:385	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	8	48	theme	chemotaxonomic	835:848	arg1	consistent					886:895	consistent	886:895	consistent	886:895	The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus.
35254234	8	48	theme	chemotaxonomic	835:848	arg1	properties					850:859	The chemotaxonomic properties	831:859	The chemotaxonomic properties of BG109T	831:869	The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus.
35254234	9	49	dep	Metabacillus	1143:1154	arg1	crassostreae					1156:1167	crassostreae	1156:1167	crassostreae	1156:1167	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	4	50	theme	concentration	392:404	arg1	presence					369:376	the presence	365:376	the presence of 0-7 % NaCl concentration	365:404	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	11	51	dep	Metabacillus	1517:1528	arg1	bambusae					1530:1537	bambusae	1530:1537	bambusae	1530:1537	Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus, for which the name Metabacillus bambusae sp.
35254234	7	52	theme	major	740:744	arg1	acids					752:756	The major fatty acids	736:756	The major fatty acids	736:756	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	52	theme	major	740:744	arg1	 0					776:777	 0	776:777	 0	776:777	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	7	52	theme	major	740:744	arg1	anteiso-C15 					763:774	anteiso-C15 	763:774	anteiso-C15 	763:774	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0, iso-C16 : 0 and C16 : 0.
35254234	4	53	theme	 NaCl	386:390	arg1	concentration					392:404	0-7 % NaCl concentration	381:404	0-7 % NaCl concentration	381:404	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	10	54	theme	genus	1368:1372	arg1	Metabacillus					1374:1385	the genus Metabacillus	1364:1385	the genus Metabacillus	1364:1385	The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus.
35254234	11	55	theme	Metabacillus	1484:1495	arg1	species					1463:1469	a novel species	1455:1469	a novel species	1455:1469	Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus, for which the name Metabacillus bambusae sp.
35254234	2	56	theme	bacterial	67:75	arg1	strain					77:82	A bacterial strain	65:82	A bacterial strain designated BG109T	65:100	A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization.
35254234	10	57	theme	Metabacillus	1374:1385	arg1	species					1353:1359	other species	1347:1359	other species of the genus Metabacillus	1347:1385	The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus.
35254234	8	58	theme	genus	926:930	arg1	Metabacillus					932:943	the genus Metabacillus	922:943	the genus Metabacillus	922:943	The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus.
35254234	9	59	theme	suggested	1225:1233	arg1	level					1242:1246	the suggested cutoff level	1221:1246	the suggested cutoff level for species distinction	1221:1270	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	60	theme	16S	968:970	arg1	similarities					991:1002	highest 16S rRNA gene sequence similarities	960:1002	highest 16S rRNA gene sequence similarities	960:1002	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	%					1188:1188	97.13 %	1182:1188	97.13 %	1182:1188	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	SW-211T					1121:1127	Metabacillus litoralis SW-211T	1098:1127	Metabacillus litoralis SW-211T (98.16 %)	1098:1137	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	%					1046:1046	99.26 %	1040:1046	99.26 %	1040:1046	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	elymi					1023:1027	'Metabacillus elymi' KUDC1714	1009:1037	'Metabacillus elymi' KUDC1714 (99.26 %)	1009:1047	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	%					1094:1094	98.17 %	1088:1094	98.17 %	1088:1094	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	100118T					1173:1179	Metabacillus crassostreae JSM 100118T	1143:1179	Metabacillus crassostreae JSM 100118T (97.13 %)	1143:1189	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	%					1136:1136	98.16 %	1130:1136	98.16 %	1130:1136	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	61	with	similarities	991:1002	arg1	DSL-17T					1079:1085	Metabacillus sediminilitoris DSL-17T	1050:1085	Metabacillus sediminilitoris DSL-17T (98.17 %)	1050:1095	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	4	62	dep	optimum	345:351	arg1	8					354:354	8	354:354	8	354:354	BG109T showed growth at 10-40 °C (optimum, 37 °C), at pH 4-10 (optimum, 8), and in the presence of 0-7 % NaCl concentration (optimum, 0-1 %).
35254234	9	63	theme	cutoff	1235:1240	arg1	level					1242:1246	the suggested cutoff level	1221:1246	the suggested cutoff level for species distinction	1221:1270	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	5	64	theme	predominant	428:438	arg1	MK-7					466:469	MK-7	466:469	MK-7	466:469	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	5	64	theme	predominant	428:438	arg1	menaquinone					440:450	The predominant menaquinone	424:450	The predominant menaquinone of BG109T	424:460	The predominant menaquinone of BG109T was MK-7, and the cell wall peptidoglycan contained major amounts of meso-diaminopimelic acid as the diagnostic diamino acid.
35254234	9	65	dep	Metabacillus	1050:1061	arg1	sediminilitoris					1063:1077	sediminilitoris	1063:1077	sediminilitoris	1063:1077	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	66	theme	sequence	982:989	arg1	similarities					991:1002	highest 16S rRNA gene sequence similarities	960:1002	highest 16S rRNA gene sequence similarities	960:1002	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	2	67	theme	taxonomic	167:175	arg1	characterization					177:192	polyphasic taxonomic characterization	156:192	polyphasic taxonomic characterization	156:192	A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization.
35254234	9	68	theme	litoralis	1111:1119	arg1	SW-211T					1121:1127	Metabacillus litoralis SW-211T	1098:1127	Metabacillus litoralis SW-211T (98.16 %)	1098:1137	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	9	68	theme	litoralis	1111:1119	arg1	%					1136:1136	98.16 %	1130:1136	98.16 %	1130:1136	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
35254234	6	69	theme	unidentified	674:685	arg1	phospholipids					687:699	unidentified phospholipids	674:699	unidentified phospholipids	674:699	The diagnostic polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and unidentified phospholipids and glycolipids were also present.
35254234	2	70	attach	isolated	106:113	arg2	strain					77:82	A bacterial strain	65:82	A bacterial strain designated BG109T	65:100	A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization.
35254234	2	70	attach	isolated	106:113	arg1	soil					133:136	bamboo grove soil	120:136	bamboo grove soil	120:136	A bacterial strain designated BG109T was isolated from bamboo grove soil, and subjected to polyphasic taxonomic characterization.
35254234	8	71	with	consistent	886:895	arg1	those					902:906	those	902:906	those	902:906	The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus.
35254234	11	72	theme	genus	1478:1482	arg1	Metabacillus					1484:1495	the genus Metabacillus	1474:1495	the genus Metabacillus	1474:1495	Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus, for which the name Metabacillus bambusae sp.
35254234	10	73	from	species	1353:1359	arg1	separation					1321:1330	the separation	1317:1330	the separation of BG109T from other species of the genus Metabacillus	1317:1385	The genome level relatedness also confirmed the separation of BG109T from other species of the genus Metabacillus.
35254234	8	74	theme	BG109T	864:869	arg1	consistent					886:895	consistent	886:895	consistent	886:895	The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus.
35254234	8	74	theme	BG109T	864:869	arg1	properties					850:859	The chemotaxonomic properties	831:859	The chemotaxonomic properties of BG109T	831:869	The chemotaxonomic properties of BG109T were generally consistent with those of members of the genus Metabacillus.
35254234	11	75	theme	novel	1457:1461	arg1	species					1463:1469	a novel species	1455:1469	a novel species	1455:1469	Thus, it is evident that BG109T merits recognition as representing a novel species of the genus Metabacillus, for which the name Metabacillus bambusae sp.
35254234	9	76	theme	species	1252:1258	arg1	distinction					1260:1270	species distinction	1252:1270	species distinction	1252:1270	BG109T shared highest 16S rRNA gene sequence similarities with 'Metabacillus elymi' KUDC1714 (99.26 %), Metabacillus sediminilitoris DSL-17T (98.17 %), Metabacillus litoralis SW-211T (98.16 %) and Metabacillus crassostreae JSM 100118T (97.13 %), all of which were well below the suggested cutoff level for species distinction.
36362241	6	0	theme	Hydrophilic	1000:1010	arg1	HILIC					1047:1051	HILIC	1047:1051	HILIC	1047:1051	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	0	theme	Hydrophilic	1000:1010	arg1	chromatography					1031:1044	Hydrophilic interaction liquid chromatography	1000:1044	Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	1000:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	7	1	theme	B-lymphocytes	1372:1384	arg1	receptors					1235:1243	Fc receptors	1232:1243	Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1232:1384	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	2	2	theme	human	391:395	arg1	IgE					397:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	9	3	theme	efficient	1579:1587	arg1	generation					1589:1598	efficient generation	1579:1598	efficient generation	1579:1598	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
36362241	6	4	theme	IgEs	992:995	arg1	analyses					952:959	Comparative glycan analyses	933:959	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	933:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	4	5	theme	tumor-associated	644:659	arg1	Receptor					751:758	Human Epidermal Growth Factor Receptor 2	721:760	Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	721:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	5	theme	tumor-associated	644:659	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	5	theme	tumor-associated	644:659	arg1	Proteoglycan					690:701	Chondroitin Sulfate Proteoglycan 4	670:703	Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE)	670:715	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	7	6	theme	Fc	1232:1233	arg1	receptors					1235:1243	Fc receptors	1232:1243	Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1232:1384	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	4	7	theme	Human	721:725	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	7	theme	Human	721:725	arg1	Receptor					751:758	Human Epidermal Growth Factor Receptor 2	721:760	Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	721:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	7	theme	Human	721:725	arg1	HER2-IgE					763:770	HER2-IgE	763:770	HER2-IgE	763:770	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	7	8	theme	leukemia	1301:1308	arg1	cells					1319:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	4	9	theme	Growth	737:742	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	9	theme	Growth	737:742	arg1	Receptor					751:758	Human Epidermal Growth Factor Receptor 2	721:760	Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	721:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	9	theme	Growth	737:742	arg1	HER2-IgE					763:770	HER2-IgE	763:770	HER2-IgE	763:770	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	9	10	theme	recombinant	1629:1639	arg1	IgEs					1674:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
36362241	6	11	theme	performance	1059:1069	arg1	HPLC					1094:1097	HPLC	1094:1097	HPLC	1094:1097	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	11	theme	performance	1059:1069	arg1	chromatography					1078:1091	-high performance liquid chromatography	1053:1091	Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	1000:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	2	12	theme	functional	318:327	arg1	analysis					329:336	functional analysis	318:336	functional analysis of native and sialic acid-deficient glyco-engineered human IgE	318:399	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	7	13	theme	FcεRI-expressing	1269:1284	arg1	cells					1319:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	1	14	theme	AllergoOncology	222:236	arg1	field					238:242	the emerging AllergoOncology field	209:242	the emerging AllergoOncology field for antibody immunotherapy	209:269	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	1	15	theme	glycan	128:133	arg1	structures					135:144	their glycan structures	122:144	their glycan structures	122:144	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	6	16	theme	liquid	1024:1029	arg1	HILIC					1047:1051	HILIC	1047:1051	HILIC	1047:1051	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	16	theme	liquid	1024:1029	arg1	chromatography					1031:1044	Hydrophilic interaction liquid chromatography	1000:1044	Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	1000:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	8	17	attach	cross-linked	1486:1497	arg3	surface					1511:1517	the cell surface	1502:1517	the cell surface	1502:1517	When cross-linked on the cell surface, both IgEs triggered RBL-SX38 degranulation.
36362241	6	18	theme	glycan	945:950	arg1	analyses					952:959	Comparative glycan analyses	933:959	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	933:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	7	19	theme	RPMI-8866	1362:1370	arg1	B-lymphocytes					1372:1384	CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1333:1384	CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1333:1384	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	5	20	theme	CSPG4-IgE	885:893	arg1	antibody					923:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	Using neuraminidase-A, we generated sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody.
36362241	1	21	from	function	182:189	arg1	allergy					194:200	allergy	194:200	allergy	194:200	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	1	21	from	function	182:189	arg1	field					238:242	the emerging AllergoOncology field	209:242	the emerging AllergoOncology field for antibody immunotherapy	209:269	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	7	22	theme	CD23/FcεRII-expressing	1333:1354	arg1	B-lymphocytes					1372:1384	CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1333:1384	CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1333:1384	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	4	23	theme	light	813:817	arg1	chains					819:824	free light chains	808:824	free light chains	808:824	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	6	24	theme	residues	1139:1146	arg1	loss					1110:1113	loss	1110:1113	loss of sialic acid terminal residues and differential glycan profiles	1110:1179	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	7	25	theme	cells	1319:1323	arg1	surface					1252:1258	the surface	1248:1258	the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1248:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	6	26	theme	profiles	1172:1179	arg1	loss					1110:1113	loss	1110:1113	loss of sialic acid terminal residues and differential glycan profiles	1110:1179	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	4	27	theme	by-products	788:798	arg1	devoid					778:783	devoid	778:783	devoid	778:783	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	5	28	theme	sialic	863:868	arg1	antibody					923:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	Using neuraminidase-A, we generated sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody.
36362241	1	29	theme	IgE	103:105	arg1	antibodies					107:116	IgE antibodies	103:116	IgE antibodies	103:116	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	6	30	theme	differential	1152:1163	arg1	profiles					1172:1179	differential glycan profiles	1152:1179	differential glycan profiles	1152:1179	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	3	31	theme	affinity	505:512	arg1	matrix					514:519	a human IgE class-specific affinity matrix	478:519	a human IgE class-specific affinity matrix	478:519	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	7	32	theme	rat	1286:1288	arg1	cells					1319:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	7	33	theme	Fab-mediated	1455:1466	arg1	recognition					1468:1478	Fab-mediated recognition	1455:1478	Fab-mediated recognition	1455:1478	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	4	34	theme	Purified	613:620	arg1	IgEs					622:625	Purified IgEs	613:625	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	613:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	3	35	theme	IgE	486:488	arg1	matrix					514:519	a human IgE class-specific affinity matrix	478:519	a human IgE class-specific affinity matrix	478:519	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	7	36	theme	human	1263:1267	arg1	cells					1319:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	0	37	theme	Native	35:40	arg1	IgE					68:70	Native and Sialic Acid-Deficient IgE	35:70	Native and Sialic Acid-Deficient IgE	35:70	Generation and Characterization of Native and Sialic Acid-Deficient IgE.
36362241	6	38	theme	Comparative	933:943	arg1	analyses					952:959	Comparative glycan analyses	933:959	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	933:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	39	theme	acid	1125:1128	arg1	residues					1139:1146	sialic acid terminal residues	1118:1146	sialic acid terminal residues	1118:1146	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	3	40	theme	embryonic	437:445	arg1	cells					454:458	human embryonic kidney cells	431:458	human embryonic kidney cells	431:458	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	0	41	theme	IgE	68:70	arg1	Characterization					15:30	Characterization	15:30	Characterization	15:30	Generation and Characterization of Native and Sialic Acid-Deficient IgE.
36362241	0	41	theme	IgE	68:70	arg1	Generation					0:9	Generation	0:9	Generation	0:9	Generation and Characterization of Native and Sialic Acid-Deficient IgE.
36362241	2	42	theme	glyco-engineered	374:389	arg1	IgE					397:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	0	43	theme	Sialic	46:51	arg1	IgE					68:70	Native and Sialic Acid-Deficient IgE	35:70	Native and Sialic Acid-Deficient IgE	35:70	Generation and Characterization of Native and Sialic Acid-Deficient IgE.
36362241	7	44	theme	melanoma	1428:1435	arg1	cells					1437:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	2	45	theme	sialic	352:357	arg1	IgE					397:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	7	46	theme	A2058	1416:1420	arg1	cells					1437:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	1	47	theme	antibody	248:255	arg1	immunotherapy					257:269	antibody immunotherapy	248:269	antibody immunotherapy	248:269	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	6	48	theme	interaction	1012:1022	arg1	HILIC					1047:1051	HILIC	1047:1051	HILIC	1047:1051	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	48	theme	interaction	1012:1022	arg1	chromatography					1031:1044	Hydrophilic interaction liquid chromatography	1000:1044	Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	1000:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	4	49	theme	Sulfate	682:688	arg1	CSPG4-IgE					706:714	CSPG4-IgE	706:714	CSPG4-IgE	706:714	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	49	theme	Sulfate	682:688	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	49	theme	Sulfate	682:688	arg1	Proteoglycan					690:701	Chondroitin Sulfate Proteoglycan 4	670:703	Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE)	670:715	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	2	50	theme	IgE	397:399	arg1	glyco-profiling					298:312	glyco-profiling	298:312	glyco-profiling	298:312	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	2	50	theme	IgE	397:399	arg1	analysis					329:336	functional analysis	318:336	functional analysis of native and sialic acid-deficient glyco-engineered human IgE	318:399	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	2	50	theme	IgE	397:399	arg1	generation					286:295	the generation	282:295	the generation	282:295	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	4	51	theme	specific	627:634	arg1	IgEs					622:625	Purified IgEs	613:625	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	613:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	6	52	theme	glyco-engineered	975:990	arg1	IgEs					992:995	native and glyco-engineered IgEs	964:995	native and glyco-engineered IgEs	964:995	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	7	53	theme	glyco-engineered	1193:1208	arg1	CSPG4-IgEs					1210:1219	Native and glyco-engineered CSPG4-IgEs	1182:1219	Native and glyco-engineered CSPG4-IgEs	1182:1219	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	3	54	theme	size-exclusion	576:589	arg1	SEC					607:609	SEC	607:609	SEC	607:609	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	3	54	theme	size-exclusion	576:589	arg1	chromatography					591:604	size-exclusion chromatography	576:604	size-exclusion chromatography (SEC)	576:610	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	9	55	theme	functional	1604:1613	arg1	competence					1615:1624	functional competence	1604:1624	functional competence	1604:1624	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
36362241	6	56	theme	native	964:969	arg1	IgEs					992:995	native and glyco-engineered IgEs	964:995	native and glyco-engineered IgEs	964:995	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	7	57	theme	Native	1182:1187	arg1	CSPG4-IgEs					1210:1219	Native and glyco-engineered CSPG4-IgEs	1182:1219	Native and glyco-engineered CSPG4-IgEs	1182:1219	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	2	58	theme	native	341:346	arg1	IgE					397:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	6	59	theme	liquid	1071:1076	arg1	HPLC					1094:1097	HPLC	1094:1097	HPLC	1094:1097	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	59	theme	liquid	1071:1076	arg1	chromatography					1078:1091	-high performance liquid chromatography	1053:1091	Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	1000:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	4	60	theme	Epidermal	727:735	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	60	theme	Epidermal	727:735	arg1	Receptor					751:758	Human Epidermal Growth Factor Receptor 2	721:760	Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	721:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	60	theme	Epidermal	727:735	arg1	HER2-IgE					763:770	HER2-IgE	763:770	HER2-IgE	763:770	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	9	61	theme	native	1641:1646	arg1	IgEs					1674:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
36362241	6	62	theme	-high	1053:1057	arg1	HPLC					1094:1097	HPLC	1094:1097	HPLC	1094:1097	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	62	theme	-high	1053:1057	arg1	chromatography					1078:1091	-high performance liquid chromatography	1053:1091	Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	1000:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	63	dep	chromatography	1031:1044	arg1	HPLC					1094:1097	HPLC	1094:1097	HPLC	1094:1097	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	6	63	dep	chromatography	1031:1044	arg1	chromatography					1078:1091	-high performance liquid chromatography	1053:1091	Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC)	1000:1098	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	5	64	theme	glyco-engineered	906:921	arg1	antibody					923:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	Using neuraminidase-A, we generated sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody.
36362241	9	65	theme	IgEs	1674:1677	arg1	generation					1589:1598	efficient generation	1579:1598	efficient generation	1579:1598	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
36362241	9	65	theme	IgEs	1674:1677	arg1	competence					1615:1624	functional competence	1604:1624	functional competence	1604:1624	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
36362241	1	66	theme	structures	135:144	arg1	characterization					83:98	Efficient characterization	73:98	Efficient characterization of IgE antibodies and their glycan structures	73:144	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	6	67	theme	sialic	1118:1123	arg1	residues					1139:1146	sialic acid terminal residues	1118:1146	sialic acid terminal residues	1118:1146	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	7	68	theme	human	1356:1360	arg1	B-lymphocytes					1372:1384	CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1333:1384	CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes	1333:1384	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	4	69	dep	antigens	661:668	arg1	CSPG4-IgE					706:714	CSPG4-IgE	706:714	CSPG4-IgE	706:714	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	69	dep	antigens	661:668	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	69	dep	antigens	661:668	arg1	Receptor					751:758	Human Epidermal Growth Factor Receptor 2	721:760	Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	721:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	69	dep	antigens	661:668	arg1	HER2-IgE					763:770	HER2-IgE	763:770	HER2-IgE	763:770	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	69	dep	antigens	661:668	arg1	Proteoglycan					690:701	Chondroitin Sulfate Proteoglycan 4	670:703	Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE)	670:715	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	70	theme	Factor	744:749	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	70	theme	Factor	744:749	arg1	Receptor					751:758	Human Epidermal Growth Factor Receptor 2	721:760	Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	721:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	70	theme	Factor	744:749	arg1	HER2-IgE					763:770	HER2-IgE	763:770	HER2-IgE	763:770	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	71	theme	free	808:811	arg1	chains					819:824	free light chains	808:824	free light chains	808:824	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	7	72	theme	RBL-SX38	1310:1317	arg1	cells					1319:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	1	73	theme	Efficient	73:81	arg1	characterization					83:98	Efficient characterization	73:98	Efficient characterization of IgE antibodies and their glycan structures	73:144	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	8	74	theme	cell	1506:1509	arg1	surface					1511:1517	the cell surface	1502:1517	the cell surface	1502:1517	When cross-linked on the cell surface, both IgEs triggered RBL-SX38 degranulation.
36362241	3	75	theme	human	480:484	arg1	matrix					514:519	a human IgE class-specific affinity matrix	478:519	a human IgE class-specific affinity matrix	478:519	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	7	76	theme	basophilic	1290:1299	arg1	cells					1319:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1263:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	6	77	theme	glycan	1165:1170	arg1	profiles					1172:1179	differential glycan profiles	1152:1179	differential glycan profiles	1152:1179	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	3	78	theme	human	431:435	arg1	cells					454:458	human embryonic kidney cells	431:458	human embryonic kidney cells	431:458	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	5	79	theme	acid-deficient	870:883	arg1	antibody					923:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody	863:930	Using neuraminidase-A, we generated sialic acid-deficient CSPG4-IgE as example glyco-engineered antibody.
36362241	1	80	theme	antibodies	107:116	arg1	characterization					83:98	Efficient characterization	73:98	Efficient characterization of IgE antibodies and their glycan structures	73:144	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	1	81	theme	emerging	213:220	arg1	field					238:242	the emerging AllergoOncology field	209:242	the emerging AllergoOncology field for antibody immunotherapy	209:269	Efficient characterization of IgE antibodies and their glycan structures is required for understanding their function in allergy and in the emerging AllergoOncology field for antibody immunotherapy.
36362241	6	82	theme	terminal	1130:1137	arg1	residues					1139:1146	sialic acid terminal residues	1118:1146	sialic acid terminal residues	1118:1146	Comparative glycan analyses of native and glyco-engineered IgEs by Hydrophilic interaction liquid chromatography (HILIC)-high performance liquid chromatography (HPLC) indicated loss of sialic acid terminal residues and differential glycan profiles.
36362241	3	83	theme	class-specific	490:503	arg1	matrix					514:519	a human IgE class-specific affinity matrix	478:519	a human IgE class-specific affinity matrix	478:519	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	0	84	theme	Acid-Deficient	53:66	arg1	IgE					68:70	Native and Sialic Acid-Deficient IgE	35:70	Native and Sialic Acid-Deficient IgE	35:70	Generation and Characterization of Native and Sialic Acid-Deficient IgE.
36362241	9	85	theme	sialic	1652:1657	arg1	IgEs					1674:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
36362241	7	86	theme	human	1422:1426	arg1	cells					1437:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	3	87	theme	kidney	447:452	arg1	cells					454:458	human embryonic kidney cells	431:458	human embryonic kidney cells	431:458	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	7	88	from	receptors	1235:1243	arg1	surface					1252:1258	the surface	1248:1258	the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells	1248:1323	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	2	89	theme	acid-deficient	359:372	arg1	IgE					397:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	native and sialic acid-deficient glyco-engineered human IgE	341:399	We report the generation, glyco-profiling and functional analysis of native and sialic acid-deficient glyco-engineered human IgE.
36362241	7	90	theme	CSPG4-expressing	1399:1414	arg1	cells					1437:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	CSPG4-expressing A2058 human melanoma cells	1399:1441	Native and glyco-engineered CSPG4-IgEs recognized Fc receptors on the surface of human FcεRI-expressing rat basophilic leukemia RBL-SX38 cells, and of CD23/FcεRII-expressing human RPMI-8866 B-lymphocytes and bound to CSPG4-expressing A2058 human melanoma cells, confirming Fab-mediated recognition.
36362241	4	91	theme	Chondroitin	670:680	arg1	CSPG4-IgE					706:714	CSPG4-IgE	706:714	CSPG4-IgE	706:714	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	91	theme	Chondroitin	670:680	arg1	antigens					661:668	the tumor-associated antigens	640:668	the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE)	640:771	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	4	91	theme	Chondroitin	670:680	arg1	Proteoglycan					690:701	Chondroitin Sulfate Proteoglycan 4	670:703	Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE)	670:715	Purified IgEs specific for the tumor-associated antigens Chondroitin Sulfate Proteoglycan 4 (CSPG4-IgE) and Human Epidermal Growth Factor Receptor 2 (HER2-IgE) were devoid of by-products such as free light chains.
36362241	3	92	theme	structural	525:534	arg1	integrity					536:544	structural integrity	525:544	structural integrity	525:544	The antibodies produced from human embryonic kidney cells were purified via a human IgE class-specific affinity matrix and structural integrity was confirmed by SDS-PAGE and size-exclusion chromatography (SEC).
36362241	9	93	theme	acid-deficient	1659:1672	arg1	IgEs					1674:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	recombinant native and sialic acid-deficient IgEs	1629:1677	We demonstrate efficient generation and functional competence of recombinant native and sialic acid-deficient IgEs.
34935826	9	0	theme	nutritional	1314:1324	arg1	supplements					1326:1336	nutritional supplements	1314:1336	nutritional supplements	1314:1336	An increase in amino acids was observed in our experiment, which may benefit nutritional supplements.
34935826	4	1	theme	activity	680:687	arg1	DAI					696:698	DAI	696:698	DAI	696:698	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	4	1	theme	activity	680:687	arg1	index					689:693	the disease activity index	668:693	the disease activity index (DAI)	668:699	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	1	2	theme	egg-milk	168:175	arg1	FEMB					187:190	FEMB	187:190	FEMB	187:190	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
34935826	1	2	theme	egg-milk	168:175	arg1	beverage					177:184	Fermented egg-milk beverage	158:184	Fermented egg-milk beverage (FEMB)	158:191	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
34935826	5	3	from	mice	771:774	arg1	form					746:749	form	746:749	form	746:749	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	5	3	from	mice	771:774	arg1	length					735:740	length	735:740	length	735:740	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	1	4	theme	intestinal	257:266	arg1	flora					268:272	intestinal flora	257:272	intestinal flora	257:272	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
34935826	9	5	from	increase	1240:1247	arg1	acids					1258:1262	amino acids	1252:1262	amino acids	1252:1262	An increase in amino acids was observed in our experiment, which may benefit nutritional supplements.
34935826	5	6	from	form	746:749	arg1	mice					771:774	the UC mice	764:774	the UC mice	764:774	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	10	7	theme	Balb/c	1420:1425	arg1	mice					1427:1430	Balb/c mice	1420:1430	Balb/c mice	1420:1430	In conclusion, FEMB treatment can alleviate the damage of DSS-induced colitis in Balb/c mice.
34935826	4	8	theme	disease	672:678	arg1	DAI					696:698	DAI	696:698	DAI	696:698	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	4	8	theme	disease	672:678	arg1	index					689:693	the disease activity index	668:693	the disease activity index (DAI)	668:699	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	2	9	from	effect	338:343	arg1	UC					413:414	UC	413:414	UC	413:414	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	2	9	from	effect	338:343	arg1	colitis					404:410	ulcerative colitis	393:410	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	356:423	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	11	10	theme	inflammation	1496:1507	arg1	application					1517:1527	the application	1513:1527	the application of FEMB	1513:1535	This study provides a theoretical basis for both the relief of inflammation and the application of FEMB.
34935826	11	10	theme	inflammation	1496:1507	arg1	relief					1486:1491	the relief	1482:1491	the relief of inflammation	1482:1507	This study provides a theoretical basis for both the relief of inflammation and the application of FEMB.
34935826	4	11	theme	FEMB	603:606	arg1	treatment					608:616	FEMB treatment	603:616	FEMB treatment for 14 days	603:628	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	0	12	from	colitis	70:76	arg1	mice					81:84	mice	81:84	mice	81:84	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	10	13	theme	colitis	1409:1415	arg1	damage					1387:1392	the damage	1383:1392	the damage of DSS-induced colitis in Balb/c mice	1383:1430	In conclusion, FEMB treatment can alleviate the damage of DSS-induced colitis in Balb/c mice.
34935826	7	14	theme	rRNA	903:906	arg1	sequencing					913:922	16S rRNA gene sequencing	899:922	16S rRNA gene sequencing	899:922	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	3	15	theme	pathological	478:489	arg1	abnormalities					491:503	the UC mice's pathological abnormalities	464:503	the UC mice's pathological abnormalities	464:503	The results showed that FEMB relieved the UC mice's pathological abnormalities and colonic inflammation, and restructured the intestinal flora composition simultaneously.
34935826	1	16	theme	supplying	278:286	arg1	nutrition					288:296	supplying nutrition	278:296	supplying nutrition	278:296	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
34935826	0	17	theme	flora	123:127	arg1	modulation					98:107	the modulation	94:107	the modulation of intestinal flora and short-chain fatty acids	94:155	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	6	18	theme	goblet	848:853	arg1	cells					855:859	goblet cells	848:859	goblet cells	848:859	Inflammatory cells decreased or disappeared, and goblet cells and crypt were enriched and modified.
34935826	7	19	theme	gene	908:911	arg1	sequencing					913:922	16S rRNA gene sequencing	899:922	16S rRNA gene sequencing	899:922	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	4	20	theme	rose	659:662	arg1	weight					640:645	the body weight	631:645	the body weight of the mice rose	631:662	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	4	20	theme	rose	659:662	arg1	DAI					696:698	DAI	696:698	DAI	696:698	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	4	20	theme	rose	659:662	arg1	index					689:693	the disease activity index	668:693	the disease activity index (DAI)	668:699	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	8	21	theme	inflammation	1211:1222	arg1	alleviation					1224:1234	inflammation alleviation	1211:1234	inflammation alleviation	1211:1234	Moreover, probiotics that can increase the content of short-chain fatty acids (SCFAs) may contribute to inflammation alleviation.
34935826	0	22	theme	intestinal	112:121	arg1	flora					123:127	intestinal flora	112:127	intestinal flora	112:127	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	10	23	theme	DSS-induced	1397:1407	arg1	colitis					1409:1415	DSS-induced colitis	1397:1415	DSS-induced colitis	1397:1415	In conclusion, FEMB treatment can alleviate the damage of DSS-induced colitis in Balb/c mice.
34935826	5	24	theme	UC	768:769	arg1	mice					771:774	the UC mice	764:774	the UC mice	764:774	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	2	25	theme	ulcerative	393:402	arg1	UC					413:414	UC	413:414	UC	413:414	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	2	25	theme	ulcerative	393:402	arg1	colitis					404:410	ulcerative colitis	393:410	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	356:423	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	0	26	theme	egg-milk	10:17	arg1	beverage					19:26	Fermented egg-milk beverage	0:26	Fermented egg-milk beverage	0:26	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	3	27	theme	colonic	509:515	arg1	inflammation					517:528	colonic inflammation	509:528	colonic inflammation	509:528	The results showed that FEMB relieved the UC mice's pathological abnormalities and colonic inflammation, and restructured the intestinal flora composition simultaneously.
34935826	7	28	theme	mice	1041:1044	arg1	content					1030:1036	the cecum content	1020:1036	the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus	1020:1104	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	7	29	theme	16S	899:901	arg1	sequencing					913:922	16S rRNA gene sequencing	899:922	16S rRNA gene sequencing	899:922	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	3	30	theme	flora	563:567	arg1	composition					569:579	the intestinal flora composition	548:579	the intestinal flora composition	548:579	The results showed that FEMB relieved the UC mice's pathological abnormalities and colonic inflammation, and restructured the intestinal flora composition simultaneously.
34935826	9	31	located	observed	1268:1275	arg1	experiment					1284:1293	our experiment	1280:1293	our experiment	1280:1293	An increase in amino acids was observed in our experiment, which may benefit nutritional supplements.
34935826	9	31	located	observed	1268:1275	arg2	increase					1240:1247	An increase	1237:1247	An increase in amino acids	1237:1262	An increase in amino acids was observed in our experiment, which may benefit nutritional supplements.
34935826	2	32	theme	-induced	384:391	arg1	UC					413:414	UC	413:414	UC	413:414	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	2	32	theme	-induced	384:391	arg1	colitis					404:410	ulcerative colitis	393:410	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	356:423	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	0	33	theme	Fermented	0:8	arg1	beverage					19:26	Fermented egg-milk beverage	0:26	Fermented egg-milk beverage	0:26	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	9	34	theme	amino	1252:1256	arg1	acids					1258:1262	amino acids	1252:1262	amino acids	1252:1262	An increase in amino acids was observed in our experiment, which may benefit nutritional supplements.
34935826	2	35	theme	protective	327:336	arg1	effect					338:343	the protective effect	323:343	the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	323:423	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	4	36	theme	mice	654:657	arg1	rose					659:662	the mice rose	650:662	the mice rose	650:662	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	0	37	theme	short-chain	133:143	arg1	acids					151:155	short-chain fatty acids	133:155	short-chain fatty acids	133:155	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	10	38	theme	FEMB	1354:1357	arg1	treatment					1359:1367	FEMB treatment	1354:1367	FEMB treatment	1354:1367	In conclusion, FEMB treatment can alleviate the damage of DSS-induced colitis in Balb/c mice.
34935826	8	39	theme	acids	1179:1183	arg1	content					1150:1156	the content	1146:1156	the content of short-chain fatty acids (SCFAs)	1146:1191	Moreover, probiotics that can increase the content of short-chain fatty acids (SCFAs) may contribute to inflammation alleviation.
34935826	6	40	theme	Inflammatory	799:810	arg1	cells					812:816	Inflammatory cells	799:816	Inflammatory cells	799:816	Inflammatory cells decreased or disappeared, and goblet cells and crypt were enriched and modified.
34935826	3	41	theme	intestinal	552:561	arg1	flora					563:567	the intestinal flora	548:567	the intestinal flora composition	548:579	The results showed that FEMB relieved the UC mice's pathological abnormalities and colonic inflammation, and restructured the intestinal flora composition simultaneously.
34935826	0	42	theme	sulfate	47:53	arg1	colitis					70:76	dextran sulfate sodium-induced colitis	39:76	dextran sulfate sodium-induced colitis in mice	39:84	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	0	43	theme	acids	151:155	arg1	modulation					98:107	the modulation	94:107	the modulation of intestinal flora and short-chain fatty acids	94:155	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	4	44	dep	weight	640:645	arg1	value					701:705	value	701:705	value	701:705	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	11	45	theme	theoretical	1455:1465	arg1	basis					1467:1471	a theoretical basis	1453:1471	a theoretical basis for both the relief of inflammation and the application of FEMB	1453:1535	This study provides a theoretical basis for both the relief of inflammation and the application of FEMB.
34935826	0	46	theme	dextran	39:45	arg1	sulfate					47:53	dextran sulfate	39:53	dextran sulfate sodium-induced colitis in mice	39:84	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	0	47	theme	fatty	145:149	arg1	acids					151:155	short-chain fatty acids	133:155	short-chain fatty acids	133:155	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	4	48	theme	body	635:638	arg1	weight					640:645	the body weight	631:645	the body weight of the mice rose	631:662	After FEMB treatment for 14 days, the body weight of the mice rose and the disease activity index (DAI) value decreased.
34935826	5	49	theme	colons	754:759	arg1	form					746:749	form	746:749	form	746:749	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	5	49	theme	colons	754:759	arg1	length					735:740	length	735:740	length	735:740	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	7	50	theme	bacteria	1008:1015	arg1	abundance					984:992	the abundance	980:992	the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus	980:1104	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	7	51	theme	cecum	1024:1028	arg1	content					1030:1036	the cecum content	1020:1036	the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus	1020:1104	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	0	52	theme	sodium-induced	55:68	arg1	colitis					70:76	dextran sulfate sodium-induced colitis	39:76	dextran sulfate sodium-induced colitis in mice	39:84	Fermented egg-milk beverage alleviates dextran sulfate sodium-induced colitis in mice through the modulation of intestinal flora and short-chain fatty acids.
34935826	5	53	dep	length	735:740	arg1	the					731:733	the	731:733	the	731:733	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	5	54	from	length	735:740	arg1	mice					771:774	the UC mice	764:774	the UC mice	764:774	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	2	55	from	colitis	404:410	arg1	mice					420:423	mice	420:423	mice	420:423	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	8	56	theme	fatty	1173:1177	arg1	acids					1179:1183	short-chain fatty acids	1161:1183	short-chain fatty acids (SCFAs)	1161:1191	Moreover, probiotics that can increase the content of short-chain fatty acids (SCFAs) may contribute to inflammation alleviation.
34935826	8	56	theme	fatty	1173:1177	arg1	SCFAs					1186:1190	SCFAs	1186:1190	SCFAs	1186:1190	Moreover, probiotics that can increase the content of short-chain fatty acids (SCFAs) may contribute to inflammation alleviation.
34935826	2	57	theme	FEMB	348:351	arg1	effect					338:343	the protective effect	323:343	the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice	323:423	This study investigated the protective effect of FEMB on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice.
34935826	11	58	theme	FEMB	1532:1535	arg1	application					1517:1527	the application	1513:1527	the application of FEMB	1513:1535	This study provides a theoretical basis for both the relief of inflammation and the application of FEMB.
34935826	11	58	theme	FEMB	1532:1535	arg1	relief					1486:1491	the relief	1482:1491	the relief of inflammation	1482:1507	This study provides a theoretical basis for both the relief of inflammation and the application of FEMB.
34935826	1	59	theme	intestinal	223:232	arg1	diseases					234:241	intestinal diseases	223:241	intestinal diseases	223:241	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
34935826	7	60	theme	beneficial	997:1006	arg1	bacteria					1008:1015	beneficial bacteria	997:1015	beneficial bacteria	997:1015	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	8	61	theme	short-chain	1161:1171	arg1	acids					1179:1183	short-chain fatty acids	1161:1183	short-chain fatty acids (SCFAs)	1161:1191	Moreover, probiotics that can increase the content of short-chain fatty acids (SCFAs) may contribute to inflammation alleviation.
34935826	8	61	theme	short-chain	1161:1171	arg1	SCFAs					1186:1190	SCFAs	1186:1190	SCFAs	1186:1190	Moreover, probiotics that can increase the content of short-chain fatty acids (SCFAs) may contribute to inflammation alleviation.
34935826	7	62	theme	FEMB	950:953	arg1	treatment					955:963	FEMB treatment	950:963	FEMB treatment	950:963	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	3	63	theme	UC	468:469	arg1	abnormalities					491:503	the UC mice's pathological abnormalities	464:503	the UC mice's pathological abnormalities	464:503	The results showed that FEMB relieved the UC mice's pathological abnormalities and colonic inflammation, and restructured the intestinal flora composition simultaneously.
34935826	1	64	theme	diseases	234:241	arg1	symptoms					211:218	the symptoms	207:218	the symptoms of intestinal diseases	207:241	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
34935826	7	65	from	abundance	984:992	arg1	content					1030:1036	the cecum content	1020:1036	the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus	1020:1104	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	10	66	from	damage	1387:1392	arg1	mice					1427:1430	Balb/c mice	1420:1430	Balb/c mice	1420:1430	In conclusion, FEMB treatment can alleviate the damage of DSS-induced colitis in Balb/c mice.
34935826	7	67	dep	results	924:930	arg1	demonstrated					932:943	demonstrated	932:943	results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus	924:1104	16S rRNA gene sequencing results demonstrated that FEMB treatment could increase the abundance of beneficial bacteria in the cecum content of mice, including unclassified_f_Lachnospiraceae and Lactobacillus.
34935826	5	68	from	colons	754:759	arg1	mice					771:774	the UC mice	764:774	the UC mice	764:774	Furthermore, the length and form of colons in the UC mice were notably restored.
34935826	1	69	theme	Fermented	158:166	arg1	FEMB					187:190	FEMB	187:190	FEMB	187:190	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
34935826	1	69	theme	Fermented	158:166	arg1	beverage					177:184	Fermented egg-milk beverage	158:184	Fermented egg-milk beverage (FEMB)	158:191	Fermented egg-milk beverage (FEMB) can alleviate the symptoms of intestinal diseases by regulating intestinal flora and supplying nutrition.
36821367	3	0	theme	internal	281:288	arg1	spacer					302:307	internal transcribed spacer	281:307	internal transcribed spacer rDNA	281:312	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	1	theme	transcribed	290:300	arg1	spacer					302:307	internal transcribed spacer	281:307	internal transcribed spacer rDNA	281:312	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	4	2	theme	glycerol	753:760	arg1	agar					762:765	dichloran 18 % glycerol agar	738:765	dichloran 18 % glycerol agar	738:765	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	3	theme	dextrose	823:830	arg1	NaCl					843:846	potato dextrose agar +20 % NaCl	816:846	potato dextrose agar +20 % NaCl	816:846	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	4	theme	potato	816:821	arg1	NaCl					843:846	potato dextrose agar +20 % NaCl	816:846	potato dextrose agar +20 % NaCl	816:846	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	2	5	theme	Aspergillus	129:139	arg1	species					141:147	a xerophilic Aspergillus species	116:147	a xerophilic Aspergillus species	116:147	Three strains of a xerophilic Aspergillus species were isolated from house dust and honey in Japan.
36821367	3	6	dep	regions	272:278	arg1	calmodulin					315:324	calmodulin	315:324	calmodulin	315:324	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	6	dep	regions	272:278	arg1	rDNA					309:312	internal transcribed spacer rDNA	281:312	internal transcribed spacer rDNA	281:312	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	6	dep	regions	272:278	arg1	polymerase					346:355	RNA polymerase II	342:358	RNA polymerase II	342:358	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	6	dep	regions	272:278	arg1	regions					272:278	four regions	267:278	four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit)	267:382	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	6	dep	regions	272:278	arg1	β-tubulin					327:335	β-tubulin	327:335	β-tubulin	327:335	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	2	7	theme	xerophilic	118:127	arg1	species					141:147	a xerophilic Aspergillus species	116:147	a xerophilic Aspergillus species	116:147	Three strains of a xerophilic Aspergillus species were isolated from house dust and honey in Japan.
36821367	4	8	theme	18 	748:750	arg1	agar					762:765	dichloran 18 % glycerol agar	738:765	dichloran 18 % glycerol agar	738:765	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	3	9	theme	section	490:496	arg1	Restricti					498:506	section Restricti	490:506	section Restricti	490:506	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	4	10	theme	agar	832:835	arg1	NaCl					843:846	potato dextrose agar +20 % NaCl	816:846	potato dextrose agar +20 % NaCl	816:846	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	11	theme	dichloran	738:746	arg1	agar					762:765	dichloran 18 % glycerol agar	738:765	dichloran 18 % glycerol agar	738:765	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	1	12	theme	house	68:72	arg1	dust					74:77	house dust	68:77	house dust	68:77	nov., a xerophilic species isolated from house dust and honey in Japan.
36821367	4	13	theme	+20 	837:840	arg1	NaCl					843:846	potato dextrose agar +20 % NaCl	816:846	potato dextrose agar +20 % NaCl	816:846	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	3	14	theme	RNA	342:344	arg1	polymerase					346:355	RNA polymerase II	342:358	RNA polymerase II	342:358	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	14	theme	RNA	342:344	arg1	regions					272:278	four regions	267:278	four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit)	267:382	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	15	theme	molecular	201:209	arg1	analysis					224:231	A molecular phylogenetic analysis	199:231	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit)	199:382	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	4	16	dep	lack	782:785	arg1	iii					773:775	iii	773:775	iii	773:775	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	6	17	theme	Aspergillus	1017:1027	arg1	sp					1040:1041	the name Aspergillus verrucosus sp	1008:1041	the name Aspergillus verrucosus sp	1008:1041	Therefore, we consider it to be a novel species and propose the name Aspergillus verrucosus sp.
36821367	4	18	theme	cleistothecia	619:631	arg1	size					611:614	the size	607:614	(i) the size of cleistothecia	603:631	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	18	theme	cleistothecia	619:631	arg1	size					671:674	size	671:674	size	671:674	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	18	theme	cleistothecia	619:631	arg1	structure					657:665	the surface structure	645:665	the surface structure	645:665	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	18	theme	cleistothecia	619:631	arg1	aspects					594:600	three aspects	588:600	three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores	588:688	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	0	19	theme	Aspergillus	0:10	arg1	sp					23:24	Aspergillus verrucosus sp	0:24	Aspergillus verrucosus sp.	0:25	Aspergillus verrucosus sp.
36821367	1	20	attach	isolated	54:61	arg2	species					46:52	a xerophilic species	33:52	a xerophilic species isolated from house dust and honey in Japan	33:96	nov., a xerophilic species isolated from house dust and honey in Japan.
36821367	1	20	attach	isolated	54:61	arg1	honey					83:87	honey	83:87	honey	83:87	nov., a xerophilic species isolated from house dust and honey in Japan.
36821367	1	20	attach	isolated	54:61	arg1	dust					74:77	house dust	68:77	house dust	68:77	nov., a xerophilic species isolated from house dust and honey in Japan.
36821367	3	21	theme	phylogenetic	211:222	arg1	analysis					224:231	A molecular phylogenetic analysis	199:231	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit)	199:382	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	5	22	theme	Aspergillus	909:919	arg1	species					939:945	all known Aspergillus section Restricti species	899:945	all known Aspergillus section Restricti species	899:945	These strains could be clearly distinguished from all known Aspergillus section Restricti species.
36821367	3	23	theme	combined	246:253	arg1	dataset					255:261	the combined dataset	242:261	the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit)	242:382	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	2	24	attach	isolated	154:161	arg1	dust					174:177	house dust	168:177	house dust	168:177	Three strains of a xerophilic Aspergillus species were isolated from house dust and honey in Japan.
36821367	2	24	attach	isolated	154:161	arg2	strains					105:111	Three strains	99:111	Three strains of a xerophilic Aspergillus species	99:147	Three strains of a xerophilic Aspergillus species were isolated from house dust and honey in Japan.
36821367	2	24	attach	isolated	154:161	arg1	honey					183:187	honey	183:187	honey	183:187	Three strains of a xerophilic Aspergillus species were isolated from house dust and honey in Japan.
36821367	6	25	theme	name	1012:1015	arg1	sp					1040:1041	the name Aspergillus verrucosus sp	1008:1041	the name Aspergillus verrucosus sp	1008:1041	Therefore, we consider it to be a novel species and propose the name Aspergillus verrucosus sp.
36821367	4	26	dep	A.	569:570	arg1	halophilicus					572:583	A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl	569:846	A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl	569:846	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	27	theme	ascospores	679:688	arg1	size					611:614	the size	607:614	(i) the size of cleistothecia	603:631	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	27	theme	ascospores	679:688	arg1	size					671:674	size	671:674	size	671:674	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	27	theme	ascospores	679:688	arg1	structure					657:665	the surface structure	645:665	the surface structure	645:665	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	27	theme	ascospores	679:688	arg1	aspects					594:600	three aspects	588:600	three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores	588:688	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	3	28	theme	independent	420:430	arg1	sister					441:446	sister	441:446	sister to Aspergillus halophilicus classified in section Restricti	441:506	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	28	theme	independent	420:430	arg1	lineage					432:438	an independent lineage	417:438	an independent lineage	417:438	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	29	theme	largest	367:373	arg1	subunit					375:381	largest subunit	367:381	internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit	281:381	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	30	dep	rDNA	309:312	arg1	subunit					375:381	largest subunit	367:381	internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit	281:381	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	2	31	theme	species	141:147	arg1	strains					105:111	Three strains	99:111	Three strains of a xerophilic Aspergillus species	99:147	Three strains of a xerophilic Aspergillus species were isolated from house dust and honey in Japan.
36821367	4	32	theme	%	751:751	arg1	agar					762:765	dichloran 18 % glycerol agar	738:765	dichloran 18 % glycerol agar	738:765	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	33	theme	surface	649:655	arg1	structure					657:665	the surface structure	645:665	the surface structure	645:665	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	33	theme	surface	649:655	arg1	aspects					594:600	three aspects	588:600	three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores	588:688	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	34	theme	formation	803:811	arg1	lack					782:785	the lack	778:785	(iii) the lack of conidiophore formation	772:811	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	34	theme	formation	803:811	arg1	agar					729:732	Harrold's agar	719:732	Harrold's agar	719:732	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	34	theme	formation	803:811	arg1	agar					762:765	dichloran 18 % glycerol agar	738:765	dichloran 18 % glycerol agar	738:765	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	35	theme	Morphological	509:521	arg1	comparisons					523:533	Morphological comparisons	509:533	Morphological comparisons	509:533	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	36	theme	conidiophore	790:801	arg1	formation					803:811	conidiophore formation	790:811	conidiophore formation	790:811	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	37	dep	aspects	594:600	arg1	size					611:614	the size	607:614	(i) the size of cleistothecia	603:631	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	37	dep	aspects	594:600	arg1	structure					657:665	the surface structure	645:665	the surface structure	645:665	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	37	dep	aspects	594:600	arg1	aspects					594:600	three aspects	588:600	three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores	588:688	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	5	38	theme	known	903:907	arg1	species					939:945	all known Aspergillus section Restricti species	899:945	all known Aspergillus section Restricti species	899:945	These strains could be clearly distinguished from all known Aspergillus section Restricti species.
36821367	5	39	theme	section	921:927	arg1	species					939:945	all known Aspergillus section Restricti species	899:945	all known Aspergillus section Restricti species	899:945	These strains could be clearly distinguished from all known Aspergillus section Restricti species.
36821367	1	40	dep	species	46:52	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a xerophilic species isolated from house dust and honey in Japan.
36821367	6	41	theme	verrucosus	1029:1038	arg1	sp					1040:1041	the name Aspergillus verrucosus sp	1008:1041	the name Aspergillus verrucosus sp	1008:1041	Therefore, we consider it to be a novel species and propose the name Aspergillus verrucosus sp.
36821367	5	42	theme	Restricti	929:937	arg1	species					939:945	all known Aspergillus section Restricti species	899:945	all known Aspergillus section Restricti species	899:945	These strains could be clearly distinguished from all known Aspergillus section Restricti species.
36821367	6	43	theme	novel	982:986	arg1	species					988:994	a novel species	980:994	a novel species	980:994	Therefore, we consider it to be a novel species and propose the name Aspergillus verrucosus sp.
36821367	6	43	theme	novel	982:986	arg1	it					971:972	it	971:972	it	971:972	Therefore, we consider it to be a novel species and propose the name Aspergillus verrucosus sp.
36821367	3	44	theme	spacer	302:307	arg1	rDNA					309:312	internal transcribed spacer rDNA	281:312	internal transcribed spacer rDNA	281:312	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	3	44	theme	spacer	302:307	arg1	regions					272:278	four regions	267:278	four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit)	267:382	A molecular phylogenetic analysis based on the combined dataset for four regions (internal transcribed spacer rDNA, calmodulin, β-tubulin, and RNA polymerase II second largest subunit) revealed that the strains formed an independent lineage, sister to Aspergillus halophilicus classified in section Restricti.
36821367	2	45	theme	house	168:172	arg1	dust					174:177	house dust	168:177	house dust	168:177	Three strains of a xerophilic Aspergillus species were isolated from house dust and honey in Japan.
36821367	4	46	dep	size	611:614	arg1	i					604:604	i	604:604	i	604:604	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	47	dep	grow	711:714	arg1	ii					692:693	ii	692:693	ii	692:693	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	4	48	theme	%	841:841	arg1	NaCl					843:846	potato dextrose agar +20 % NaCl	816:846	potato dextrose agar +20 % NaCl	816:846	Morphological comparisons show that the strains differ from A. halophilicus in three aspects: (i) the size of cleistothecia, as well as the surface structure and size of ascospores, (ii) the ability to grow on Harrold's agar and dichloran 18 % glycerol agar, and (iii) the lack of conidiophore formation on potato dextrose agar +20 % NaCl.
36821367	1	49	theme	xerophilic	35:44	arg1	species					46:52	a xerophilic species	33:52	a xerophilic species isolated from house dust and honey in Japan	33:96	nov., a xerophilic species isolated from house dust and honey in Japan.
36821367	0	50	theme	verrucosus	12:21	arg1	sp					23:24	Aspergillus verrucosus sp	0:24	Aspergillus verrucosus sp.	0:25	Aspergillus verrucosus sp.
35164146	5	0	theme	epirubicin-HCl	907:920	arg1	dispersion					893:902	The dispersion	889:902	The dispersion of epirubicin-HCl in the hydrogel matrix	889:943	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	1	1	theme	drug-delivery	181:193	arg1	platforms					195:203	drug-delivery platforms	181:203	drug-delivery platforms	181:203	3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks.
35164146	0	2	theme	Epirubicin-HCl	87:100	arg1	Delivery					102:109	Epirubicin-HCl Delivery	87:109	Epirubicin-HCl Delivery	87:109	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	7	3	from	change	1199:1204	arg1	pattern					1224:1230	the expression pattern	1209:1230	the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers	1209:1294	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	3	4	theme	critical	533:540	arg1	combinations					542:553	The critical combinations	529:553	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time)	529:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	0	5	theme	MCF-7	114:118	arg1	Cells					120:124	MCF-7 Cells	114:124	MCF-7 Cells	114:124	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	5	6	theme	3D-printed	1037:1046	arg1	platforms					1062:1070	3D-printed drug-delivery platforms	1037:1070	3D-printed drug-delivery platforms	1037:1070	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	3	7	theme	calcium	568:574	arg1	carbonate					576:584	calcium carbonate	568:584	calcium carbonate	568:584	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	5	8	theme	alginate-based	995:1008	arg1	ink					1010:1012	this self-crosslinking alginate-based ink	972:1012	this self-crosslinking alginate-based ink	972:1012	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	1	9	theme	innovative	308:317	arg1	inks					319:322	innovative inks	308:322	innovative inks	308:322	3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks.
35164146	4	10	theme	3D	779:780	arg1	extrusion					782:790	3D extrusion	779:790	3D extrusion	779:790	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	3	11	theme	carbonate	576:584	arg1	combinations					542:553	The critical combinations	529:553	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time)	529:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	1	12	theme	inks	319:322	arg1	development					293:303	the development	289:303	the development of innovative inks	289:322	3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks.
35164146	7	13	theme	anti-apoptotic	1235:1248	arg1	markers					1288:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	0	14	from	Cryoprinting	54:65	arg1	Cells					120:124	MCF-7 Cells	114:124	MCF-7 Cells	114:124	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	4	15	theme	good	863:866	arg1	properties					877:886	good handling properties	863:886	good handling properties	863:886	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	6	16	theme	cytotoxic	1135:1143	arg1	activity					1145:1152	the cytotoxic activity	1131:1152	the cytotoxic activity	1131:1152	Drug release from 3D-printed hydrogels was monitored, and the cytotoxic activity was tested against MCF-7 cells.
35164146	3	17	theme	alginate	558:565	arg1	combinations					542:553	The critical combinations	529:553	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time)	529:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	2	18	theme	alginate	425:432	arg1	gelation					409:416	the internal gelation	396:416	the internal gelation of the alginate	396:432	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	5	19	theme	hydrogel	929:936	arg1	matrix					938:943	the hydrogel matrix	925:943	the hydrogel matrix	925:943	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	2	20	theme	epirubicin-HCl	507:520	arg1	loading					496:502	the loading	492:502	the loading of epirubicin-HCl (EPI)	492:526	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	6	21	from	hydrogels	1102:1110	arg1	release					1078:1084	Drug release	1073:1084	Drug release from 3D-printed hydrogels	1073:1110	Drug release from 3D-printed hydrogels was monitored, and the cytotoxic activity was tested against MCF-7 cells.
35164146	7	22	theme	pro-apoptotic	1251:1263	arg1	markers					1288:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	0	23	theme	Self-Crosslinking	9:25	arg1	Ink					27:29	Alginate Self-Crosslinking Ink	0:29	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.	0:125	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	7	24	theme	autophagy	1270:1278	arg1	markers					1288:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	5	25	theme	drug-delivery	1048:1060	arg1	platforms					1062:1070	3D-printed drug-delivery platforms	1037:1070	3D-printed drug-delivery platforms	1037:1070	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	2	26	theme	3D	348:349	arg1	approach					367:374	a new 3D extrusion-based approach	342:374	a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI)	342:526	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	0	27	theme	Alginate	0:7	arg1	Ink					27:29	Alginate Self-Crosslinking Ink	0:29	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.	0:125	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	4	28	theme	handling	868:875	arg1	properties					877:886	good handling properties	863:886	good handling properties	863:886	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	2	29	theme	new	344:346	arg1	approach					367:374	a new 3D extrusion-based approach	342:374	a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI)	342:526	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	4	30	theme	hydrogels	828:836	arg1	preparation					804:814	the preparation	800:814	the preparation of alginate hydrogels	800:836	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	4	31	from	gelation	755:762	arg1	tandem					767:772	tandem	767:772	tandem	767:772	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	5	32	theme	self-crosslinking	977:993	arg1	ink					1010:1012	this self-crosslinking alginate-based ink	972:1012	this self-crosslinking alginate-based ink	972:1012	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	1	33	theme	water-soluble	228:240	arg1	compounds					249:257	water-soluble active compounds	228:257	water-soluble active compounds	228:257	3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks.
35164146	2	34	theme	crosslinking	460:471	arg1	process					473:479	the post-printing crosslinking process	442:479	the post-printing crosslinking process	442:479	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	3	35	theme	d-glucono-δ-lactone	590:608	arg1	combinations					542:553	The critical combinations	529:553	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time)	529:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	1	36	theme	active	242:247	arg1	compounds					249:257	water-soluble active compounds	228:257	water-soluble active compounds	228:257	3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks.
35164146	0	37	theme	Extrusion-Based	38:52	arg1	Cryoprinting					54:65	3D Extrusion-Based Cryoprinting	35:65	3D Extrusion-Based Cryoprinting	35:65	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	5	38	theme	platforms	1062:1070	arg1	preparation					1022:1032	the preparation	1018:1032	the preparation of 3D-printed drug-delivery platforms	1018:1070	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	7	39	theme	MCF-7	1378:1382	arg1	exposure					1366:1373	exposure	1366:1373	exposure of MCF-7 to the EPI-loaded hydrogels	1366:1410	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	3	40	dep	parameters	654:663	arg1	temperature					682:692	temperature	682:692	temperature	682:692	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	40	dep	parameters	654:663	arg1	parameters					654:663	the scaffold production parameters	630:663	the scaffold production parameters (extrusion time, temperature, and curing time)	630:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	40	dep	parameters	654:663	arg1	time					676:679	extrusion time	666:679	extrusion time	666:679	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	40	dep	parameters	654:663	arg1	time					706:709	curing time	699:709	curing time	699:709	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	0	41	theme	3D	35:36	arg1	Cryoprinting					54:65	3D Extrusion-Based Cryoprinting	35:65	3D Extrusion-Based Cryoprinting	35:65	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	3	42	theme	extrusion	666:674	arg1	parameters					654:663	the scaffold production parameters	630:663	the scaffold production parameters (extrusion time, temperature, and curing time)	630:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	42	theme	extrusion	666:674	arg1	time					676:679	extrusion time	666:679	extrusion time	666:679	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	6	43	theme	3D-printed	1091:1100	arg1	hydrogels					1102:1110	3D-printed hydrogels	1091:1110	3D-printed hydrogels	1091:1110	Drug release from 3D-printed hydrogels was monitored, and the cytotoxic activity was tested against MCF-7 cells.
35164146	4	44	with	tandem	767:772	arg1	extrusion					782:790	3D extrusion	779:790	3D extrusion	779:790	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	1	45	with	loading	215:221	arg1	compounds					249:257	water-soluble active compounds	228:257	water-soluble active compounds	228:257	3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks.
35164146	0	46	from	Application	71:81	arg1	Cells					120:124	MCF-7 Cells	114:124	MCF-7 Cells	114:124	Alginate Self-Crosslinking Ink for 3D Extrusion-Based Cryoprinting and Application for Epirubicin-HCl Delivery on MCF-7 Cells.
35164146	1	47	theme	3D-printed	127:136	arg1	hydrogels					138:146	3D-printed hydrogels	127:146	3D-printed hydrogels	127:146	3D-printed hydrogels are particularly advantageous as drug-delivery platforms but their loading with water-soluble active compounds remains a challenge requiring the development of innovative inks.
35164146	3	48	theme	curing	699:704	arg1	parameters					654:663	the scaffold production parameters	630:663	the scaffold production parameters (extrusion time, temperature, and curing time)	630:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	48	theme	curing	699:704	arg1	time					706:709	curing time	699:709	curing time	699:709	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	4	49	theme	complex	845:851	arg1	shape					853:857	a complex shape	843:857	a complex shape	843:857	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	7	50	theme	EPI-loaded	1391:1400	arg1	hydrogels					1402:1410	the EPI-loaded hydrogels	1387:1410	the EPI-loaded hydrogels	1387:1410	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	4	51	theme	alginate	819:826	arg1	hydrogels					828:836	alginate hydrogels	819:836	alginate hydrogels	819:836	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	7	52	theme	liquid-chromatography	1313:1333	arg1	tandem-mass-spectrometry					1335:1358	liquid-chromatography tandem-mass-spectrometry	1313:1358	liquid-chromatography tandem-mass-spectrometry	1313:1358	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	4	53	theme	internal	746:753	arg1	gelation					755:762	The internal gelation	742:762	The internal gelation in tandem with 3D extrusion	742:790	The internal gelation in tandem with 3D extrusion allowed the preparation of alginate hydrogels with a complex shape and good handling properties.
35164146	6	54	theme	MCF-7	1173:1177	arg1	cells					1179:1183	MCF-7 cells	1173:1183	MCF-7 cells	1173:1183	Drug release from 3D-printed hydrogels was monitored, and the cytotoxic activity was tested against MCF-7 cells.
35164146	5	55	theme	ink	1010:1012	arg1	potential					959:967	the potential	955:967	the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms	955:1070	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	5	56	from	dispersion	893:902	arg1	matrix					938:943	the hydrogel matrix	925:943	the hydrogel matrix	925:943	The dispersion of epirubicin-HCl in the hydrogel matrix confirmed the potential of this self-crosslinking alginate-based ink for the preparation of 3D-printed drug-delivery platforms.
35164146	2	57	theme	extrusion-based	351:365	arg1	approach					367:374	a new 3D extrusion-based approach	342:374	a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI)	342:526	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	7	58	theme	protein	1280:1286	arg1	markers					1288:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	anti-apoptotic, pro-apoptotic, and autophagy protein markers	1235:1294	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	3	59	theme	scaffold	634:641	arg1	temperature					682:692	temperature	682:692	temperature	682:692	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	59	theme	scaffold	634:641	arg1	parameters					654:663	the scaffold production parameters	630:663	the scaffold production parameters (extrusion time, temperature, and curing time)	630:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	59	theme	scaffold	634:641	arg1	time					676:679	extrusion time	666:679	extrusion time	666:679	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	59	theme	scaffold	634:641	arg1	time					706:709	curing time	699:709	curing time	699:709	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	2	60	theme	post-printing	446:458	arg1	process					473:479	the post-printing crosslinking process	442:479	the post-printing crosslinking process	442:479	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	2	61	theme	internal	400:407	arg1	gelation					409:416	the internal gelation	396:416	the internal gelation of the alginate	396:432	Here, we propose a new 3D extrusion-based approach that, by exploiting the internal gelation of the alginate, avoids the post-printing crosslinking process and allows the loading of epirubicin-HCl (EPI).
35164146	6	62	theme	Drug	1073:1076	arg1	release					1078:1084	Drug release	1073:1084	Drug release from 3D-printed hydrogels	1073:1110	Drug release from 3D-printed hydrogels was monitored, and the cytotoxic activity was tested against MCF-7 cells.
35164146	7	63	theme	markers	1288:1294	arg1	pattern					1224:1230	the expression pattern	1209:1230	the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers	1209:1294	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
35164146	3	64	theme	production	643:652	arg1	temperature					682:692	temperature	682:692	temperature	682:692	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	64	theme	production	643:652	arg1	parameters					654:663	the scaffold production parameters	630:663	the scaffold production parameters (extrusion time, temperature, and curing time)	630:710	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	64	theme	production	643:652	arg1	time					676:679	extrusion time	666:679	extrusion time	666:679	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	3	64	theme	production	643:652	arg1	time					706:709	curing time	699:709	curing time	699:709	The critical combinations of alginate, calcium carbonate and d-glucono-δ-lactone (GDL) combined with the scaffold production parameters (extrusion time, temperature, and curing time) were evaluated and discussed.
35164146	7	65	theme	expression	1213:1222	arg1	pattern					1224:1230	the expression pattern	1209:1230	the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers	1209:1294	Finally, the change in the expression pattern of anti-apoptotic, pro-apoptotic, and autophagy protein markers was monitored by liquid-chromatography tandem-mass-spectrometry after exposure of MCF-7 to the EPI-loaded hydrogels.
37306942	6	0	theme	related	1231:1237	arg1	symptoms					1222:1229	clinical symptoms	1213:1229	clinical symptoms related to inflammatory colon diseases	1213:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	1	theme	interest	1025:1032	arg1	worthy					1015:1020	worthy	1015:1020	worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases	1015:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	0	2	theme	antioxidant	79:89	arg1	properties					132:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	0	3	from	characterization	9:24	arg1	properties					132:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	0	3	from	characterization	9:24	arg1	annua					63:67	Artemisia annua	53:67	Artemisia annua	53:67	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	2	4	theme	stress	549:554	arg1	burden					512:517	the burden	508:517	the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS	508:595	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	1	5	theme	Asteraceae	164:173	arg1	Family					175:180	Asteraceae Family	164:180	Asteraceae Family	164:180	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	5	theme	Asteraceae	164:173	arg1	L					160:160	Artemisia annua L	144:160	Artemisia annua L. (Asteraceae Family)	144:181	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	6	theme	Chinese	232:238	arg1	herbalists					240:249	Chinese herbalists	232:249	Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation	232:405	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	0	7	theme	inhibitory	99:108	arg1	properties					132:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	3	8	theme	antiradical	633:643	arg1	effects					668:674	chemical composition, antiradical, and enzyme inhibition effects	611:674	chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases	611:740	In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated.
37306942	2	9	theme	oxidative	539:547	arg1	stress					549:554	oxidative stress	539:554	oxidative stress	539:554	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	2	10	theme	polarity	468:475	arg1	extracts					477:484	different polarity extracts	458:484	different polarity extracts from A. annua	458:498	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	0	11	from	annua	63:67	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.	0:142	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	0	11	from	annua	63:67	arg1	extracts					39:46	different extracts	29:46	different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties	29:141	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	4	12	theme	anti-inflammatory	830:846	arg1	agents					848:853	anti-inflammatory agents	830:853	anti-inflammatory agents	830:853	All extracts revealed effective as antioxidants, enzyme inhibitors, and anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression.
37306942	6	13	theme	in	1287:1288	arg1	studies					1295:1301	future in vivo studies	1280:1301	future in vivo studies	1280:1301	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	0	14	theme	anti-inflammatory	114:130	arg1	properties					132:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	their antioxidant, enzyme inhibitory and anti-inflammatory properties	73:141	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	4	15	theme	TNFα	909:912	arg1	expression					919:928	TNFα gene expression	909:928	TNFα gene expression	909:928	All extracts revealed effective as antioxidants, enzyme inhibitors, and anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression.
37306942	3	16	theme	enzyme	650:655	arg1	inhibition					657:666	enzyme inhibition	650:666	enzyme inhibition	650:666	In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated.
37306942	2	17	theme	different	458:466	arg1	extracts					477:484	different polarity extracts	458:484	different polarity extracts from A. annua	458:498	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	3	18	theme	inhibition	657:666	arg1	effects					668:674	chemical composition, antiradical, and enzyme inhibition effects	611:674	chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases	611:740	In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated.
37306942	2	19	theme	colon	569:573	arg1	tissue					575:580	colon tissue	569:580	colon tissue exposed to LPS	569:595	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	0	20	theme	Chemical	0:7	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.	0:142	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	4	21	theme	enzyme	807:812	arg1	inhibitors					814:823	enzyme inhibitors	807:823	enzyme inhibitors	807:823	All extracts revealed effective as antioxidants, enzyme inhibitors, and anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression.
37306942	3	22	theme	chemical	611:618	arg1	composition					620:630	chemical composition	611:630	chemical composition	611:630	In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated.
37306942	6	23	theme	symptoms	1222:1229	arg1	management					1199:1208	the management	1195:1208	the management of clinical symptoms related to inflammatory colon diseases	1195:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	0	24	theme	different	29:37	arg1	extracts					39:46	different extracts	29:46	different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties	29:141	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	4	25	theme	blunting	879:886	arg1	effects					888:894	the blunting effects	875:894	the blunting effects on COX-2 and TNFα gene expression	875:928	All extracts revealed effective as antioxidants, enzyme inhibitors, and anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression.
37306942	5	26	theme	phenolic	982:989	arg1	content					991:997	the only phenolic content	973:997	the only phenolic content	973:997	These effects seemed to be not related to the only phenolic content.
37306942	6	27	from	application	1180:1190	arg1	management					1199:1208	the management	1195:1208	the management of clinical symptoms related to inflammatory colon diseases	1195:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	28	theme	gene	1090:1093	arg1	expression					1095:1104	LPS-induced gene expression	1078:1104	LPS-induced gene expression	1078:1104	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	29	theme	future	1280:1285	arg1	studies					1295:1301	future in vivo studies	1280:1301	future in vivo studies	1280:1301	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	1	30	theme	endemic	194:200	arg1	L					160:160	Artemisia annua L	144:160	Artemisia annua L. (Asteraceae Family)	144:181	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	30	theme	endemic	194:200	arg1	plant					188:192	a plant	186:192	a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation	186:405	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	pain					361:364	pain	361:364	pain	361:364	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	inflammation					394:405	inflammation	394:405	inflammation	394:405	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	wounds					330:335	wounds	330:335	wounds	330:335	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	fever					308:312	fever	308:312	fever due to malaria	308:327	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	tubercolisis					338:349	tubercolisis	338:349	tubercolisis	338:349	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	convulsions					367:377	convulsions	367:377	convulsions	367:377	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	diabetes					380:387	diabetes	380:387	diabetes	380:387	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	scabues					352:358	scabues	352:358	scabues	352:358	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	31	theme	different	278:286	arg1	diseases					288:295	different diseases	278:295	different diseases	278:295	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	4	32	from	effects	888:894	arg1	COX-2					899:903	COX-2	899:903	COX-2	899:903	All extracts revealed effective as antioxidants, enzyme inhibitors, and anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression.
37306942	4	32	from	effects	888:894	arg1	expression					919:928	TNFα gene expression	909:928	TNFα gene expression	909:928	All extracts revealed effective as antioxidants, enzyme inhibitors, and anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression.
37306942	6	33	theme	phytotherapy	1167:1178	arg1	application					1180:1190	a potential phytotherapy application	1155:1190	a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases	1155:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	34	dep	in	1330:1331	arg1	vitro					1333:1337	vitro	1333:1337	vitro	1333:1337	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	0	35	theme	extracts	39:46	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.	0:142	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	6	36	dep	ex	1343:1344	arg1	vivo					1346:1349	vivo	1346:1349	vivo	1346:1349	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	37	theme	ex	1343:1344	arg1	observations					1351:1362	such in vitro and ex vivo observations	1325:1362	such in vitro and ex vivo observations	1325:1362	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	2	38	from	effects	447:453	arg1	annua					494:498	annua	494:498	annua	494:498	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	6	39	theme	potential	1157:1165	arg1	application					1180:1190	a potential phytotherapy application	1155:1190	a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases	1155:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	40	dep	in	1287:1288	arg1	vivo					1290:1293	vivo	1290:1293	vivo	1290:1293	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	2	41	theme	extracts	477:484	arg1	effects					447:453	the effects	443:453	the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS	443:595	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	2	42	theme	inflammation	522:533	arg1	burden					512:517	the burden	508:517	the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS	508:595	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	1	43	from	Asia	205:208	arg1	endemic					194:200	endemic	194:200	endemic	194:200	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	0	44	theme	Artemisia	53:61	arg1	annua					63:67	Artemisia annua	53:67	Artemisia annua	53:67	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	6	45	theme	colon	1255:1259	arg1	diseases					1261:1268	inflammatory colon diseases	1242:1268	inflammatory colon diseases	1242:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	1	46	used	used	224:227	arg2	Asia					205:208	Asia	205:208	Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation	205:405	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	5	47	theme	only	977:980	arg1	content					991:997	the only phenolic content	973:997	the only phenolic content	973:997	These effects seemed to be not related to the only phenolic content.
37306942	6	48	theme	higher	1055:1060	arg1	potency					1062:1068	the higher potency	1051:1068	the higher potency against LPS-induced gene expression	1051:1104	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	4	49	theme	gene	914:917	arg1	expression					919:928	TNFα gene expression	909:928	TNFα gene expression	909:928	All extracts revealed effective as antioxidants, enzyme inhibitors, and anti-inflammatory agents, as demonstrated by the blunting effects on COX-2 and TNFα gene expression.
37306942	6	50	theme	water	1123:1127	arg1	extract 					1129:1136	the water extract 	1119:1136	the water extract 	1119:1136	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	51	theme	inflammatory	1242:1253	arg1	diseases					1261:1268	inflammatory colon diseases	1242:1268	inflammatory colon diseases	1242:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	3	52	theme	composition	620:630	arg1	effects					668:674	chemical composition, antiradical, and enzyme inhibition effects	611:674	chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases	611:740	In parallel, chemical composition, antiradical, and enzyme inhibition effects against α-amylase, α-glucosidase, tyrosinase, and cholinesterases were evaluated.
37306942	1	53	from	endemic	194:200	arg1	Asia					205:208	Asia	205:208	Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation	205:405	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	54	theme	Artemisia	144:152	arg1	Family					175:180	Asteraceae Family	164:180	Asteraceae Family	164:180	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	54	theme	Artemisia	144:152	arg1	plant					188:192	a plant	186:192	a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation	186:405	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	54	theme	Artemisia	144:152	arg1	L					160:160	Artemisia annua L	144:160	Artemisia annua L. (Asteraceae Family)	144:181	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	6	55	theme	LPS-induced	1078:1088	arg1	expression					1095:1104	LPS-induced gene expression	1078:1104	LPS-induced gene expression	1078:1104	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	1	56	theme	annua	154:158	arg1	Family					175:180	Asteraceae Family	164:180	Asteraceae Family	164:180	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	56	theme	annua	154:158	arg1	plant					188:192	a plant	186:192	a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation	186:405	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	1	56	theme	annua	154:158	arg1	L					160:160	Artemisia annua L	144:160	Artemisia annua L. (Asteraceae Family)	144:181	Artemisia annua L. (Asteraceae Family) is a plant endemic in Asia that has been used by Chinese herbalists for centuries for treating different diseases, including fever due to malaria, wounds, tubercolisis, scabues, pain, convulsions, diabetes, and inflammation.
37306942	6	57	theme	in	1330:1331	arg1	observations					1351:1362	such in vitro and ex vivo observations	1325:1362	such in vitro and ex vivo observations	1325:1362	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	6	58	theme	such	1325:1328	arg1	observations					1351:1362	such in vitro and ex vivo observations	1325:1362	such in vitro and ex vivo observations	1325:1362	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	2	59	from	annua	494:498	arg1	effects					447:453	the effects	443:453	the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS	443:595	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	2	59	from	annua	494:498	arg1	extracts					477:484	different polarity extracts	458:484	different polarity extracts from A. annua	458:498	In this study we aimed to evaluate the effects of different polarity extracts from A. annua against the burden of inflammation and oxidative stress occurring in colon tissue exposed to LPS.
37306942	6	60	theme	clinical	1213:1220	arg1	symptoms					1222:1229	clinical symptoms	1213:1229	clinical symptoms related to inflammatory colon diseases	1213:1268	However, it is worthy of interest to highlight how the higher potency against LPS-induced gene expression was shown by the water extract ; thus suggesting a potential phytotherapy application in the management of clinical symptoms related to inflammatory colon diseases, although future in vivo studies are needed to confirm such in vitro and ex vivo observations.
37306942	0	61	from	properties	132:141	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.	0:142	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
37306942	0	61	from	properties	132:141	arg1	extracts					39:46	different extracts	29:46	different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties	29:141	Chemical characterization of different extracts from Artemisia annua and their antioxidant, enzyme inhibitory and anti-inflammatory properties.
35234605	3	0	attach	isolated	246:253	arg2	strains					201:207	Two Gram-positive, endospore-forming, rod-shaped bacterial strains	142:207	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T	142:239	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T were isolated from plant roots.
35234605	3	0	attach	isolated	246:253	arg1	roots					266:270	plant roots	260:270	plant roots	260:270	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T were isolated from plant roots.
35234605	15	1	theme	DLE-14T=KCTC	1897:1908	arg1	34504T					1921:1926	DLE-14T=KCTC 43288T=JCM 34504T	1897:1926	type strain: DLE-14T=KCTC 43288T=JCM 34504T	1884:1926	nov. (type strain: DLE-14T=KCTC 43288T=JCM 34504T) are proposed.
35234605	12	2	theme	strains	1454:1460	arg1	genomes					1435:1441	The genomes	1431:1441	The genomes of the two strains	1431:1460	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	5	3	theme	orthologous	518:528	arg1	values					592:597	The orthologous average nucleotide identity and digital DNA-DNA hybridization values	514:597	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data	514:621	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	12	4	theme	potential	1471:1479	arg1	fixation					1537:1544	nitrogen fixation	1528:1544	nitrogen fixation	1528:1544	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	12	4	theme	potential	1471:1479	arg1	production					1559:1568	siderophore production	1547:1568	siderophore production	1547:1568	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	12	4	theme	potential	1471:1479	arg1	solubilization					1584:1597	phosphate solubilization	1574:1597	phosphate solubilization	1574:1597	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	12	4	theme	potential	1471:1479	arg1	characteristics					1504:1518	potential plant-growth-promoting characteristics	1471:1518	potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization	1471:1597	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	11	5	theme	DNA	1345:1347	arg1	%					1415:1415	60.9 and 50.8 mol%	1398:1415	60.9 and 50.8 mol%	1398:1415	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	11	5	theme	DNA	1345:1347	arg1	contents					1353:1360	The DNA G+C contents	1341:1360	The DNA G+C contents of strain MWE-103T and DLE-14T	1341:1391	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	7	6	theme	fatty	843:847	arg1	 0					887:888	 0	887:888	 0	887:888	The major fatty acid in both strains was anteiso-C15 : 0, but their relative proportions differed.
35234605	7	6	theme	fatty	843:847	arg1	acid					849:852	The major fatty acid	833:852	The major fatty acid in both strains	833:868	The major fatty acid in both strains was anteiso-C15 : 0, but their relative proportions differed.
35234605	13	7	dep	sp	1787:1788	arg1	names					1753:1757	the names	1749:1757	the names	1749:1757	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	8	8	theme	diamino	1005:1011	arg1	acid					1042:1045	meso-diaminopimelic acid	1022:1045	meso-diaminopimelic acid	1022:1045	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	8	8	theme	diamino	1005:1011	arg1	acid					1013:1016	the cell-wall diamino acid	991:1016	the cell-wall diamino acid	991:1016	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	11	9	theme	strain	1365:1370	arg1	MWE-103T					1372:1379	strain MWE-103T	1365:1379	strain MWE-103T	1365:1379	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	14	10	dep	sp	1874:1875	arg1	lignilyticus					1861:1872	Paenibacillus lignilyticus	1847:1872	Paenibacillus lignilyticus	1847:1872	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	14	10	dep	sp	1874:1875	arg1	strain					1802:1807	type strain	1797:1807	type strain: MWE-103T=KCTC 43287T=JCM 34503T	1797:1840	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	14	10	dep	sp	1874:1875	arg1	nov					1791:1793	nov	1791:1793	nov	1791:1793	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	11	11	theme	50.8 mol	1407:1414	arg1	%					1415:1415	60.9 and 50.8 mol%	1398:1415	60.9 and 50.8 mol%	1398:1415	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	11	11	theme	50.8 mol	1407:1414	arg1	contents					1353:1360	The DNA G+C contents	1341:1360	The DNA G+C contents of strain MWE-103T and DLE-14T	1341:1391	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	2	12	theme	bacterial	97:105	arg1	nov.					72:75	nov.	72:75	nov.	72:75	nov., two new endophytic bacterial species isolated from plant roots.
35234605	2	12	theme	bacterial	97:105	arg1	species					107:113	two new endophytic bacterial species	78:113	two new endophytic bacterial species	78:113	nov., two new endophytic bacterial species isolated from plant roots.
35234605	5	13	theme	DNA-DNA	570:576	arg1	hybridization					578:590	digital DNA-DNA hybridization	562:590	digital DNA-DNA hybridization	562:590	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	4	14	theme	rhizoryzae	470:479	arg1	IZS3-5T					481:487	Paenibacillus rhizoryzae IZS3-5T	456:487	Paenibacillus rhizoryzae IZS3-5T	456:487	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	2	15	theme	new	82:84	arg1	nov.					72:75	nov.	72:75	nov.	72:75	nov., two new endophytic bacterial species isolated from plant roots.
35234605	2	15	theme	new	82:84	arg1	species					107:113	two new endophytic bacterial species	78:113	two new endophytic bacterial species	78:113	nov., two new endophytic bacterial species isolated from plant roots.
35234605	15	16	dep	strain	1889:1894	arg1	34504T					1921:1926	DLE-14T=KCTC 43288T=JCM 34504T	1897:1926	type strain: DLE-14T=KCTC 43288T=JCM 34504T	1884:1926	nov. (type strain: DLE-14T=KCTC 43288T=JCM 34504T) are proposed.
35234605	6	17	theme	NaCl	827:830	arg1	absence					816:822	the absence	812:822	the absence of NaCl	812:830	Both strains grew at mesophilic temperature ranges, and grew best at pH 6 and in the absence of NaCl.
35234605	1	18	dep	sp	68:69	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov. and Paenibacillus lignilyticus sp.
35234605	1	18	dep	sp	68:69	arg1	lignilyticus					55:66	Paenibacillus lignilyticus	41:66	Paenibacillus lignilyticus	41:66	nov. and Paenibacillus lignilyticus sp.
35234605	5	19	theme	nucleotide	538:547	arg1	identity					549:556	average nucleotide identity	530:556	average nucleotide identity	530:556	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	4	20	theme	strain	438:443	arg1	DLE-14T					445:451	strain DLE-14T	438:451	strain DLE-14T	438:451	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	3	21	theme	Gram-positive	146:158	arg1	strains					201:207	Two Gram-positive, endospore-forming, rod-shaped bacterial strains	142:207	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T	142:239	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T were isolated from plant roots.
35234605	8	22	with	consistent	1171:1180	arg1	those					1187:1191	those	1187:1191	those	1187:1191	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	5	23	theme	MWE-103T	646:653	arg1	strains					638:644	strains	638:644	strains MWE-103T and DLE-14T	638:665	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	11	24	theme	G+C	1349:1351	arg1	%					1415:1415	60.9 and 50.8 mol%	1398:1415	60.9 and 50.8 mol%	1398:1415	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	11	24	theme	G+C	1349:1351	arg1	contents					1353:1360	The DNA G+C contents	1341:1360	The DNA G+C contents of strain MWE-103T and DLE-14T	1341:1391	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	5	25	theme	whole	605:609	arg1	data					618:621	whole genome data	605:621	whole genome data	605:621	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	13	26	dep	strains	1647:1653	arg1	DLE-14T					1668:1674	DLE-14T	1668:1674	DLE-14T	1668:1674	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	13	26	dep	strains	1647:1653	arg1	MWE-103T					1655:1662	MWE-103T	1655:1662	MWE-103T	1655:1662	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	13	26	dep	strains	1647:1653	arg1	strains					1647:1653	strains MWE-103T and DLE-14T	1647:1674	strains MWE-103T and DLE-14T	1647:1674	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	4	27	theme	%	500:500	arg1	similarity					502:511	99.09 % similarity	494:511	99.09 % similarity	494:511	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	13	28	theme	Paenibacillus	1759:1771	arg1	sp					1787:1788	Paenibacillus artemisiicola sp	1759:1788	Paenibacillus artemisiicola sp	1759:1788	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	0	29	theme	Paenibacillus	0:12	arg1	sp					28:29	Paenibacillus artemisiicola sp	0:29	Paenibacillus artemisiicola sp.	0:30	Paenibacillus artemisiicola sp.
35234605	8	30	theme	diagnostic	1056:1065	arg1	diphosphatidylglycerol					1085:1106	diphosphatidylglycerol	1085:1106	diphosphatidylglycerol	1085:1106	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	8	30	theme	diagnostic	1056:1065	arg1	lipids					1073:1078	the diagnostic polar lipids	1052:1078	the diagnostic polar lipids	1052:1078	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	4	31	theme	16S	277:279	arg1	rRNA					281:284	The 16S rRNA	273:284	The 16S rRNA gene sequence analysis	273:307	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	13	32	theme	Paenibacillus	1724:1736	arg1	species					1713:1719	a novel species	1705:1719	a novel species	1705:1719	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	13	32	theme	Paenibacillus	1724:1736	arg1	strains					1647:1653	strains MWE-103T and DLE-14T	1647:1674	strains MWE-103T and DLE-14T	1647:1674	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	4	33	dep	Paenibaillus	363:374	arg1	sacheonensis					376:387	sacheonensis	376:387	sacheonensis	376:387	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	12	34	theme	phosphate	1574:1582	arg1	solubilization					1584:1597	phosphate solubilization	1574:1597	phosphate solubilization	1574:1597	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	4	35	theme	gene	286:289	arg1	analysis					300:307	The 16S rRNA gene sequence analysis	273:307	The 16S rRNA gene sequence analysis	273:307	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	3	36	dep	Gram-positive	146:158	arg1	rod-shaped					180:189	rod-shaped	180:189	rod-shaped	180:189	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T were isolated from plant roots.
35234605	3	36	dep	Gram-positive	146:158	arg1	endospore-forming					161:177	endospore-forming	161:177	endospore-forming	161:177	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T were isolated from plant roots.
35234605	14	37	theme	Paenibacillus	1847:1859	arg1	lignilyticus					1861:1872	Paenibacillus lignilyticus	1847:1872	Paenibacillus lignilyticus	1847:1872	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	8	38	theme	predominant	936:946	arg1	menaquinone					976:986	menaquinone 7	976:988	menaquinone 7	976:988	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	8	38	theme	predominant	936:946	arg1	quinone					948:954	The predominant quinone	932:954	The predominant quinone of both strains	932:970	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	14	39	theme	43287T=JCM	1824:1833	arg1	34503T					1835:1840	MWE-103T=KCTC 43287T=JCM 34503T	1810:1840	type strain: MWE-103T=KCTC 43287T=JCM 34503T	1797:1840	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	8	40	theme	strains	964:970	arg1	menaquinone					976:986	menaquinone 7	976:988	menaquinone 7	976:988	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	8	40	theme	strains	964:970	arg1	quinone					948:954	The predominant quinone	932:954	The predominant quinone of both strains	932:970	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	15	41	theme	43288T=JCM	1910:1919	arg1	34504T					1921:1926	DLE-14T=KCTC 43288T=JCM 34504T	1897:1926	type strain: DLE-14T=KCTC 43288T=JCM 34504T	1884:1926	nov. (type strain: DLE-14T=KCTC 43288T=JCM 34504T) are proposed.
35234605	6	42	theme	temperature	763:773	arg1	ranges					775:780	mesophilic temperature ranges	752:780	mesophilic temperature ranges	752:780	Both strains grew at mesophilic temperature ranges, and grew best at pH 6 and in the absence of NaCl.
35234605	12	43	theme	nitrogen	1528:1535	arg1	fixation					1537:1544	nitrogen fixation	1528:1544	nitrogen fixation	1528:1544	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	9	44	theme	Amylase	1213:1219	arg1	activities					1235:1244	Amylase and cellulase activities	1213:1244	Amylase and cellulase activities	1213:1244	Amylase and cellulase activities were positive for both strains.
35234605	14	45	theme	type	1797:1800	arg1	strain					1802:1807	type strain	1797:1807	type strain: MWE-103T=KCTC 43287T=JCM 34503T	1797:1840	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	14	45	theme	type	1797:1800	arg1	nov					1791:1793	nov	1791:1793	nov	1791:1793	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	4	46	theme	%	431:431	arg1	similarity					411:420	a sequence similarity	400:420	a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity	400:511	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	15	47	theme	type	1884:1887	arg1	nov					1878:1880	nov	1878:1880	nov	1878:1880	nov. (type strain: DLE-14T=KCTC 43288T=JCM 34504T) are proposed.
35234605	15	47	theme	type	1884:1887	arg1	strain					1889:1894	type strain	1884:1894	type strain: DLE-14T=KCTC 43288T=JCM 34504T	1884:1926	nov. (type strain: DLE-14T=KCTC 43288T=JCM 34504T) are proposed.
35234605	5	48	theme	DLE-14T	659:665	arg1	strains					638:644	strains	638:644	strains MWE-103T and DLE-14T	638:665	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	9	49	theme	cellulase	1225:1233	arg1	activities					1235:1244	Amylase and cellulase activities	1213:1244	Amylase and cellulase activities	1213:1244	Amylase and cellulase activities were positive for both strains.
35234605	8	50	theme	related	1196:1202	arg1	species					1204:1210	related species	1196:1210	related species	1196:1210	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	12	51	theme	plant-growth-promoting	1481:1502	arg1	fixation					1537:1544	nitrogen fixation	1528:1544	nitrogen fixation	1528:1544	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	12	51	theme	plant-growth-promoting	1481:1502	arg1	production					1559:1568	siderophore production	1547:1568	siderophore production	1547:1568	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	12	51	theme	plant-growth-promoting	1481:1502	arg1	solubilization					1584:1597	phosphate solubilization	1574:1597	phosphate solubilization	1574:1597	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	12	51	theme	plant-growth-promoting	1481:1502	arg1	characteristics					1504:1518	potential plant-growth-promoting characteristics	1471:1518	potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization	1471:1597	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	8	52	theme	cell-wall	995:1003	arg1	acid					1042:1045	meso-diaminopimelic acid	1022:1045	meso-diaminopimelic acid	1022:1045	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	8	52	theme	cell-wall	995:1003	arg1	acid					1013:1016	the cell-wall diamino acid	991:1016	the cell-wall diamino acid	991:1016	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	11	53	theme	MWE-103T	1372:1379	arg1	%					1415:1415	60.9 and 50.8 mol%	1398:1415	60.9 and 50.8 mol%	1398:1415	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	11	53	theme	MWE-103T	1372:1379	arg1	contents					1353:1360	The DNA G+C contents	1341:1360	The DNA G+C contents of strain MWE-103T and DLE-14T	1341:1391	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	5	54	theme	average	530:536	arg1	identity					549:556	average nucleotide identity	530:556	average nucleotide identity	530:556	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	3	55	theme	plant	260:264	arg1	roots					266:270	plant roots	260:270	plant roots	260:270	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T were isolated from plant roots.
35234605	4	56	theme	sequence	402:409	arg1	similarity					411:420	a sequence similarity	400:420	a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity	400:511	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	4	57	theme	Paenibacillus	456:468	arg1	IZS3-5T					481:487	Paenibacillus rhizoryzae IZS3-5T	456:487	Paenibacillus rhizoryzae IZS3-5T	456:487	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	11	58	theme	60.9 and	1398:1405	arg1	%					1415:1415	60.9 and 50.8 mol%	1398:1415	60.9 and 50.8 mol%	1398:1415	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	11	58	theme	60.9 and	1398:1405	arg1	contents					1353:1360	The DNA G+C contents	1341:1360	The DNA G+C contents of strain MWE-103T and DLE-14T	1341:1391	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	13	59	theme	phenotypic	1626:1635	arg1	evidence					1637:1644	phylogenetic and phenotypic evidence	1609:1644	phylogenetic and phenotypic evidence	1609:1644	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	7	60	theme	major	837:841	arg1	 0					887:888	 0	887:888	 0	887:888	The major fatty acid in both strains was anteiso-C15 : 0, but their relative proportions differed.
35234605	7	60	theme	major	837:841	arg1	acid					849:852	The major fatty acid	833:852	The major fatty acid in both strains	833:868	The major fatty acid in both strains was anteiso-C15 : 0, but their relative proportions differed.
35234605	11	61	theme	DLE-14T	1385:1391	arg1	%					1415:1415	60.9 and 50.8 mol%	1398:1415	60.9 and 50.8 mol%	1398:1415	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	11	61	theme	DLE-14T	1385:1391	arg1	contents					1353:1360	The DNA G+C contents	1341:1360	The DNA G+C contents of strain MWE-103T and DLE-14T	1341:1391	The DNA G+C contents of strain MWE-103T and DLE-14T were 60.9 and 50.8 mol% respectively.
35234605	2	62	theme	endophytic	86:95	arg1	nov.					72:75	nov.	72:75	nov.	72:75	nov., two new endophytic bacterial species isolated from plant roots.
35234605	2	62	theme	endophytic	86:95	arg1	species					107:113	two new endophytic bacterial species	78:113	two new endophytic bacterial species	78:113	nov., two new endophytic bacterial species isolated from plant roots.
35234605	5	63	theme	related	714:720	arg1	species					722:728	the mostly related species	703:728	the mostly related species	703:728	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	5	64	theme	hybridization	578:590	arg1	values					592:597	The orthologous average nucleotide identity and digital DNA-DNA hybridization values	514:597	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data	514:621	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	10	65	theme	Strain	1278:1283	arg1	DLE-14T					1285:1291	Strain DLE-14T	1278:1291	Strain DLE-14T	1278:1291	Strain DLE-14T exhibited the potential for lignin degradation.
35234605	4	66	theme	DLE-14T	445:451	arg1	similarity					411:420	a sequence similarity	400:420	a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity	400:511	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	13	67	theme	phylogenetic	1609:1620	arg1	evidence					1637:1644	phylogenetic and phenotypic evidence	1609:1644	phylogenetic and phenotypic evidence	1609:1644	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	5	68	theme	identity	549:556	arg1	values					592:597	The orthologous average nucleotide identity and digital DNA-DNA hybridization values	514:597	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data	514:621	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	14	69	dep	strain	1802:1807	arg1	34503T					1835:1840	MWE-103T=KCTC 43287T=JCM 34503T	1810:1840	type strain: MWE-103T=KCTC 43287T=JCM 34503T	1797:1840	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
35234605	8	70	theme	meso-diaminopimelic	1022:1040	arg1	acid					1042:1045	meso-diaminopimelic acid	1022:1045	meso-diaminopimelic acid	1022:1045	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	8	70	theme	meso-diaminopimelic	1022:1040	arg1	acid					1013:1016	the cell-wall diamino acid	991:1016	the cell-wall diamino acid	991:1016	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	5	71	theme	digital	562:568	arg1	hybridization					578:590	digital DNA-DNA hybridization	562:590	digital DNA-DNA hybridization	562:590	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	4	72	theme	99.09 	494:499	arg1	%					500:500	%	500:500	%	500:500	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	6	73	theme	mesophilic	752:761	arg1	ranges					775:780	mesophilic temperature ranges	752:780	mesophilic temperature ranges	752:780	Both strains grew at mesophilic temperature ranges, and grew best at pH 6 and in the absence of NaCl.
35234605	4	74	theme	sequence	291:298	arg1	analysis					300:307	The 16S rRNA gene sequence analysis	273:307	The 16S rRNA gene sequence analysis	273:307	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	10	75	theme	lignin	1321:1326	arg1	degradation					1328:1338	lignin degradation	1321:1338	lignin degradation	1321:1338	Strain DLE-14T exhibited the potential for lignin degradation.
35234605	0	76	theme	artemisiicola	14:26	arg1	sp					28:29	Paenibacillus artemisiicola sp	0:29	Paenibacillus artemisiicola sp.	0:30	Paenibacillus artemisiicola sp.
35234605	8	77	theme	polar	1067:1071	arg1	diphosphatidylglycerol					1085:1106	diphosphatidylglycerol	1085:1106	diphosphatidylglycerol	1085:1106	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	8	77	theme	polar	1067:1071	arg1	lipids					1073:1078	the diagnostic polar lipids	1052:1078	the diagnostic polar lipids	1052:1078	The predominant quinone of both strains was menaquinone 7, the cell-wall diamino acid was meso-diaminopimelic acid, and the diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol, which were consistent with those of related species.
35234605	5	78	theme	genome	611:616	arg1	data					618:621	whole genome data	605:621	whole genome data	605:621	The orthologous average nucleotide identity and digital DNA-DNA hybridization values using whole genome data indicated that strains MWE-103T and DLE-14T could be readily distinguished from the mostly related species.
35234605	13	79	theme	artemisiicola	1773:1785	arg1	sp					1787:1788	Paenibacillus artemisiicola sp	1759:1788	Paenibacillus artemisiicola sp	1759:1788	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	3	80	theme	bacterial	191:199	arg1	strains					201:207	Two Gram-positive, endospore-forming, rod-shaped bacterial strains	142:207	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T	142:239	Two Gram-positive, endospore-forming, rod-shaped bacterial strains designated MWE-103T and DLE-14T were isolated from plant roots.
35234605	4	81	with	similarity	411:420	arg1	similarity					502:511	99.09 % similarity	494:511	99.09 % similarity	494:511	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	4	82	theme	rRNA	281:284	arg1	analysis					300:307	The 16S rRNA gene sequence analysis	273:307	The 16S rRNA gene sequence analysis	273:307	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	13	83	dep	recognized	1691:1700	arg1	each					1683:1686	each	1683:1686	each	1683:1686	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	4	84	with	SY01T	389:393	arg1	similarity					411:420	a sequence similarity	400:420	a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity	400:511	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	13	85	theme	novel	1707:1711	arg1	species					1713:1719	a novel species	1705:1719	a novel species	1705:1719	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	13	85	theme	novel	1707:1711	arg1	strains					1647:1653	strains MWE-103T and DLE-14T	1647:1674	strains MWE-103T and DLE-14T	1647:1674	Based on phylogenetic and phenotypic evidence, strains MWE-103T and DLE-14T should each be recognized as a novel species of Paenibacillus, for which the names Paenibacillus artemisiicola sp.
35234605	7	86	from	acid	849:852	arg1	strains					862:868	both strains	857:868	both strains	857:868	The major fatty acid in both strains was anteiso-C15 : 0, but their relative proportions differed.
35234605	7	87	theme	relative	901:908	arg1	proportions					910:920	their relative proportions	895:920	their relative proportions	895:920	The major fatty acid in both strains was anteiso-C15 : 0, but their relative proportions differed.
35234605	4	88	theme	Paenibaillus	363:374	arg1	SY01T					389:393	Paenibaillus sacheonensis SY01T	363:393	Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity	363:511	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	12	89	theme	siderophore	1547:1557	arg1	production					1559:1568	siderophore production	1547:1568	siderophore production	1547:1568	The genomes of the two strains revealed potential plant-growth-promoting characteristics such as nitrogen fixation, siderophore production and phosphate solubilization.
35234605	1	90	theme	Paenibacillus	41:53	arg1	lignilyticus					55:66	Paenibacillus lignilyticus	41:66	Paenibacillus lignilyticus	41:66	nov. and Paenibacillus lignilyticus sp.
35234605	4	91	theme	strain	324:329	arg1	MWE-103T					331:338	strain MWE-103T	324:338	strain MWE-103T	324:338	The 16S rRNA gene sequence analysis indicated that strain MWE-103T was closely related to Paenibaillus sacheonensis SY01T with a sequence similarity of 97.82 %, and strain DLE-14T to Paenibacillus rhizoryzae IZS3-5T with 99.09 % similarity.
35234605	2	92	theme	plant	129:133	arg1	roots					135:139	plant roots	129:139	plant roots	129:139	nov., two new endophytic bacterial species isolated from plant roots.
35234605	14	93	theme	MWE-103T=KCTC	1810:1822	arg1	34503T					1835:1840	MWE-103T=KCTC 43287T=JCM 34503T	1810:1840	type strain: MWE-103T=KCTC 43287T=JCM 34503T	1797:1840	nov. (type strain: MWE-103T=KCTC 43287T=JCM 34503T) and Paenibacillus lignilyticus sp.
36796433	3	0	theme	male	607:610	arg1	rats					619:622	Old male Wistar rats	603:622	Old male Wistar rats (n = 96, 18 mo old)	603:642	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	8	1	theme	protein	1500:1506	arg1	synthesis					1508:1516	Muscle protein synthesis	1493:1516	Muscle protein synthesis	1493:1516	Muscle protein synthesis was significantly stimulated by feeding to the same extent in all groups (+13%).
36796433	5	2	theme	repeated	1123:1130	arg1	measures					1132:1139	repeated measures	1123:1139	repeated measures	1123:1139	Two-factor ANOVA and repeated measures 2-factor ANOVA were conducted.
36796433	4	3	theme	flooding	1029:1036	arg1	synthesis					1018:1026	in vivo muscle protein synthesis	995:1026	in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine)	995:1062	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	4	3	theme	flooding	1029:1036	arg1	dose					1038:1041	flooding dose	1029:1041	flooding dose of L-[1-13C	1029:1053	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	10	4	theme	concept	1891:1897	arg1	proof					1882:1886	significant proof	1870:1886	significant proof of concept from the nutritional standpoint	1870:1929	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	7	5	theme	standard	1415:1422	arg1	diet					1431:1434	the standard energy diet	1411:1434	the standard energy diet	1411:1434	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	4	6	theme	liver	1076:1080	arg1	weights					1093:1099	muscle, liver, and heart weights	1068:1099	muscle, liver, and heart weights	1068:1099	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	6	7	theme	protein	1212:1218	arg1	type					1220:1223	protein type	1212:1223	protein type	1212:1223	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	1	8	theme	BACKGROUND	131:140	arg1	sources					181:187	BACKGROUND Alternative, sustainable, and adequate sources	131:187	BACKGROUND Alternative, sustainable, and adequate sources of protein	131:198	BACKGROUND Alternative, sustainable, and adequate sources of protein must be found to meet global demand.
36796433	7	9	theme	high-energy	1319:1329	arg1	diet					1331:1334	The high-energy diet	1315:1334	The high-energy diet	1315:1334	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	9	10	theme	plant	1786:1790	arg1	blend					1800:1804	our plant protein blend	1782:1804	our plant protein blend	1782:1804	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	6	11	theme	mass	1270:1273	arg1	aging					1251:1255	aging	1251:1255	aging of lean body mass, muscle mass, and muscle functionality	1251:1312	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	2	12	theme	protein	437:443	arg1	mass					445:448	muscle protein mass	430:448	muscle protein mass	430:448	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	10	13	theme	significant	1870:1880	arg1	proof					1882:1886	significant proof	1870:1886	significant proof of concept from the nutritional standpoint	1870:1929	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	6	14	theme	muscle	1276:1281	arg1	mass					1283:1286	muscle mass	1276:1286	muscle mass	1276:1286	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	2	15	theme	diet	589:592	arg1	quality					563:569	the quality	559:569	the quality of the background diet	559:592	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	2	16	dep	OBJECTIVES	237:246	arg1	was					256:258	was	256:258	was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet	256:592	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	4	17	theme	muscle	1068:1073	arg1	weights					1093:1099	muscle, liver, and heart weights	1068:1099	muscle, liver, and heart weights	1068:1099	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	0	18	theme	Fat	99:101	arg1	Intakes					113:119	Fat and Sugar Intakes	99:119	Fat and Sugar Intakes	99:119	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	3	19	dep	source	725:730	arg1	blend					755:759	plant protein blend	741:759	plant protein blend	741:759	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	19	dep	source	725:730	arg1	milk					733:736	milk	733:736	milk	733:736	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	4	20	dep	dose	1038:1041	arg1	-valine					1055:1061	-valine	1055:1061	-valine	1055:1061	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	10	21	theme	plant	1958:1962	arg1	proteins					1964:1971	appropriately blended plant proteins	1936:1971	appropriately blended plant proteins	1936:1971	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	0	22	theme	Sugar	107:111	arg1	Intakes					113:119	Fat and Sugar Intakes	99:119	Fat and Sugar Intakes	99:119	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	6	23	theme	lean	1260:1263	arg1	mass					1270:1273	lean body mass	1260:1273	lean body mass	1260:1273	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	7	24	theme	body	1360:1363	arg1	fat					1365:1367	body fat	1360:1367	body fat (+47%)	1360:1374	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	7	24	theme	body	1360:1363	arg1	%					1373:1373	+47%	1370:1373	+47%	1370:1373	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	5	25	dep	ANOVA	1113:1117	arg1	ANOVA					1150:1154	2-factor ANOVA	1141:1154	2-factor ANOVA	1141:1154	Two-factor ANOVA and repeated measures 2-factor ANOVA were conducted.
36796433	2	26	theme	cysteine	399:406	arg1	balance					318:324	a good balance	311:324	a good balance of indispensable amino acids	311:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	2	26	theme	cysteine	399:406	arg1	contents					364:371	high contents	359:371	high contents of leucine, arginine, and cysteine	359:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	9	27	contain	had	1635:1637	arg1	CONCLUSIONS					1599:1609	CONCLUSIONS	1599:1609	CONCLUSIONS Since high-energy diets	1599:1633	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	9	27	contain	had	1635:1637	arg2	impact					1646:1651	little impact	1639:1651	little impact	1639:1651	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	3	28	dep	rats	619:622	arg1	n					625:625	n = 96	625:630	n = 96	625:630	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	7	29	theme	heart	1380:1384	arg1	weight					1386:1391	heart weight	1380:1391	heart weight (+8%)	1380:1397	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	7	29	theme	heart	1380:1384	arg1	%					1396:1396	+8%	1394:1396	+8%	1394:1396	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	2	30	from	effect	274:279	arg1	maintenance					415:425	the maintenance	411:425	the maintenance of muscle protein mass and function	411:461	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	4	31	theme	body	896:899	arg1	composition					901:911	body composition	896:911	body composition	896:911	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	3	32	dep	n	625:625	arg1	old					639:641	old	639:641	old	639:641	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	9	33	theme	related	1680:1686	arg1	metabolism					1688:1697	related metabolism	1680:1697	related metabolism	1680:1697	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	0	34	theme	Free	66:69	arg1	Mass					71:74	Fat Free Mass	62:74	Fat Free Mass in Old Rats	62:86	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	5	35	theme	2-factor	1141:1148	arg1	ANOVA					1150:1154	2-factor ANOVA	1141:1154	2-factor ANOVA	1141:1154	Two-factor ANOVA and repeated measures 2-factor ANOVA were conducted.
36796433	4	36	theme	muscle	961:966	arg1	mo					957:958	4 mo	955:958	4 mo	955:958	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	4	36	theme	muscle	961:966	arg1	functionality					968:980	muscle functionality	961:980	muscle functionality	961:980	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	6	37	theme	muscle	1293:1298	arg1	functionality					1300:1312	muscle functionality	1293:1312	muscle functionality	1293:1312	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	2	38	theme	high	359:362	arg1	contents					364:371	high contents	359:371	high contents of leucine, arginine, and cysteine	359:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	9	39	theme	insulin	1656:1662	arg1	sensitivity					1664:1674	insulin sensitivity	1656:1674	insulin sensitivity	1656:1674	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	3	40	theme	Wistar	612:617	arg1	rats					619:622	Old male Wistar rats	603:622	Old male Wistar rats (n = 96, 18 mo old)	603:642	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	2	41	theme	blend	300:304	arg1	effect					274:279	the effect	270:279	the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function	270:461	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	0	42	theme	Plant	0:4	arg1	Protein					6:12	Plant Protein	0:12	Plant Protein	0:12	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	3	43	theme	saturated	843:851	arg1	fat					853:855	saturated fat	843:855	saturated fat	843:855	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	44	theme	plant	741:745	arg1	blend					755:759	plant protein blend	741:759	plant protein blend	741:759	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	2	45	theme	acids	349:353	arg1	balance					318:324	a good balance	311:324	a good balance of indispensable amino acids	311:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	2	45	theme	acids	349:353	arg1	contents					364:371	high contents	359:371	high contents of leucine, arginine, and cysteine	359:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	10	46	theme	blended	1950:1956	arg1	proteins					1964:1971	appropriately blended plant proteins	1936:1971	appropriately blended plant proteins	1936:1971	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	2	47	theme	indispensable	329:341	arg1	acids					349:353	indispensable amino acids	329:353	indispensable amino acids	329:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	1	48	theme	global	222:227	arg1	demand					229:234	global demand	222:234	global demand	222:234	BACKGROUND Alternative, sustainable, and adequate sources of protein must be found to meet global demand.
36796433	3	49	dep	METHODS	595:601	arg1	allocated					658:666	allocated	658:666	were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose)	644:868	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	7	50	contain	had	1440:1442	arg1	diet					1331:1334	The high-energy diet	1315:1334	The high-energy diet	1315:1334	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	7	50	contain	had	1440:1442	arg2	effect					1447:1452	no effect	1444:1452	no effect	1444:1452	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	9	51	theme	insulin	1762:1768	arg1	resistance					1770:1779	higher insulin resistance	1755:1779	higher insulin resistance	1755:1779	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	7	52	theme	plasma	1465:1470	arg1	glucose					1472:1478	fasting plasma glucose	1457:1478	fasting plasma glucose	1457:1478	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	6	53	dep	RESULTS	1172:1178	arg1	was					1186:1188	was	1186:1188	was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality	1186:1312	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	2	54	theme	plant	286:290	arg1	blend					300:304	a plant protein blend	284:304	a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine	284:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	3	55	with	standard	782:789	arg1	sucrose					861:867	sucrose	861:867	sucrose	861:867	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	55	with	standard	782:789	arg1	fat					853:855	saturated fat	843:855	saturated fat	843:855	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	2	56	with	blend	300:304	arg1	balance					318:324	a good balance	311:324	a good balance of indispensable amino acids	311:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	2	56	with	blend	300:304	arg1	contents					364:371	high contents	359:371	high contents of leucine, arginine, and cysteine	359:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	10	57	theme	high	1982:1985	arg1	value					1999:2003	high nutritional value	1982:2003	high nutritional value	1982:2003	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	8	58	theme	Muscle	1493:1498	arg1	synthesis					1508:1516	Muscle protein synthesis	1493:1516	Muscle protein synthesis	1493:1516	Muscle protein synthesis was significantly stimulated by feeding to the same extent in all groups (+13%).
36796433	9	59	theme	resistance	1770:1779	arg1	situations					1741:1750	situations	1741:1750	situations of higher insulin resistance	1741:1779	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	1	60	theme	protein	192:198	arg1	sources					181:187	BACKGROUND Alternative, sustainable, and adequate sources	131:187	BACKGROUND Alternative, sustainable, and adequate sources of protein	131:198	BACKGROUND Alternative, sustainable, and adequate sources of protein must be found to meet global demand.
36796433	2	61	theme	milk	493:496	arg1	proteins					498:505	milk proteins	493:505	milk proteins	493:505	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	4	62	theme	L-[1-13C	1046:1053	arg1	synthesis					1018:1026	in vivo muscle protein synthesis	995:1026	in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine)	995:1062	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	4	62	theme	L-[1-13C	1046:1053	arg1	dose					1038:1041	flooding dose	1029:1041	flooding dose of L-[1-13C	1029:1053	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	10	63	contain	have	1977:1980	arg2	value					1999:2003	high nutritional value	1982:2003	high nutritional value	1982:2003	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	10	63	contain	have	1977:1980	arg1	proteins					1964:1971	appropriately blended plant proteins	1936:1971	appropriately blended plant proteins	1936:1971	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	9	64	theme	high-energy	1617:1627	arg1	diets					1629:1633	high-energy diets	1617:1633	high-energy diets	1617:1633	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	7	65	theme	energy	1424:1429	arg1	diet					1431:1434	the standard energy diet	1411:1434	the standard energy diet	1411:1434	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	4	66	theme	protein	1010:1016	arg1	synthesis					1018:1026	in vivo muscle protein synthesis	995:1026	in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine)	995:1062	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	4	66	theme	protein	1010:1016	arg1	dose					1038:1041	flooding dose	1029:1041	flooding dose of L-[1-13C	1029:1053	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	3	67	theme	Old	603:605	arg1	rats					619:622	Old male Wistar rats	603:622	Old male Wistar rats (n = 96, 18 mo old)	603:642	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	68	theme	=	627:627	arg1	n					625:625	n = 96	625:630	n = 96	625:630	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	5	69	theme	Two-factor	1102:1111	arg1	ANOVA					1113:1117	Two-factor ANOVA	1102:1117	Two-factor ANOVA	1102:1117	Two-factor ANOVA and repeated measures 2-factor ANOVA were conducted.
36796433	1	70	theme	Alternative	142:152	arg1	sources					181:187	BACKGROUND Alternative, sustainable, and adequate sources	131:187	BACKGROUND Alternative, sustainable, and adequate sources of protein	131:198	BACKGROUND Alternative, sustainable, and adequate sources of protein must be found to meet global demand.
36796433	2	71	theme	mass	445:448	arg1	maintenance					415:425	the maintenance	411:425	the maintenance of muscle protein mass and function	411:461	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	3	72	dep	content	773:779	arg1	kcal/g					830:835	4.9 kcal/g	826:835	4.9 kcal/g	826:835	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	72	dep	content	773:779	arg1	standard					782:789	standard	782:789	standard	782:789	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	72	dep	content	773:779	arg1	kcal/g					796:801	3.6 kcal/g	792:801	3.6 kcal/g	792:801	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	72	dep	content	773:779	arg1	high					820:823	high	820:823	high	820:823	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	9	73	theme	protein	1792:1798	arg1	blend					1800:1804	our plant protein blend	1782:1804	our plant protein blend	1782:1804	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	8	74	theme	same	1565:1568	arg1	extent					1570:1575	the same extent	1561:1575	the same extent	1561:1575	Muscle protein synthesis was significantly stimulated by feeding to the same extent in all groups (+13%).
36796433	1	75	theme	sustainable	155:165	arg1	sources					181:187	BACKGROUND Alternative, sustainable, and adequate sources	131:187	BACKGROUND Alternative, sustainable, and adequate sources of protein	131:198	BACKGROUND Alternative, sustainable, and adequate sources of protein must be found to meet global demand.
36796433	2	76	theme	muscle	430:435	arg1	mass					445:448	muscle protein mass	430:448	muscle protein mass	430:448	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	4	77	theme	plasma	917:922	arg1	biochemistry					924:935	plasma biochemistry	917:935	plasma biochemistry	917:935	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	2	78	theme	background	578:587	arg1	diet					589:592	the background diet	574:592	the background diet	574:592	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	6	79	theme	body	1265:1268	arg1	mass					1270:1273	lean body mass	1260:1273	lean body mass	1260:1273	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	2	80	theme	function	454:461	arg1	maintenance					415:425	the maintenance	411:425	the maintenance of muscle protein mass and function	411:461	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	0	81	from	Mass	71:74	arg1	Rats					83:86	Old Rats	79:86	Old Rats	79:86	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	9	82	theme	milk	1825:1828	arg1	protein					1830:1836	milk protein	1825:1836	milk protein	1825:1836	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	9	83	theme	higher	1755:1760	arg1	resistance					1770:1779	higher insulin resistance	1755:1779	higher insulin resistance	1755:1779	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	10	84	theme	nutritional	1987:1997	arg1	value					1999:2003	high nutritional value	1982:2003	high nutritional value	1982:2003	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	0	85	theme	Fat	62:64	arg1	Mass					71:74	Fat Free Mass	62:74	Fat Free Mass in Old Rats	62:86	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	2	86	theme	arginine	385:392	arg1	balance					318:324	a good balance	311:324	a good balance of indispensable amino acids	311:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	2	86	theme	arginine	385:392	arg1	contents					364:371	high contents	359:371	high contents of leucine, arginine, and cysteine	359:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	4	87	theme	heart	1087:1091	arg1	weights					1093:1099	muscle, liver, and heart weights	1068:1099	muscle, liver, and heart weights	1068:1099	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	4	88	dep	in	995:996	arg1	vivo					998:1001	vivo	998:1001	vivo	998:1001	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	10	89	theme	demanding	2013:2021	arg1	metabolism					2056:2065	aging protein metabolism	2042:2065	aging protein metabolism	2042:2065	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	10	89	theme	demanding	2013:2021	arg1	situations					2023:2032	demanding situations	2013:2032	demanding situations such as aging protein metabolism	2013:2065	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	9	90	theme	little	1639:1644	arg1	impact					1646:1651	little impact	1639:1651	little impact	1639:1651	CONCLUSIONS Since high-energy diets had little impact on insulin sensitivity and related metabolism, we could not test the hypothesis that in situations of higher insulin resistance, our plant protein blend may be better than milk protein.
36796433	0	91	theme	Old	79:81	arg1	Rats					83:86	Old Rats	79:86	Old Rats	79:86	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	6	92	theme	functionality	1300:1312	arg1	aging					1251:1255	aging	1251:1255	aging of lean body mass, muscle mass, and muscle functionality	1251:1312	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	10	93	theme	rat	1853:1855	arg1	study					1857:1861	this rat study	1848:1861	this rat study	1848:1861	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	6	94	from	difference	1193:1202	arg1	maintenance					1232:1242	the maintenance	1228:1242	the maintenance during aging of lean body mass, muscle mass, and muscle functionality	1228:1312	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	3	95	theme	protein	717:723	arg1	source					725:730	protein source	717:730	protein source (milk or plant protein blend)	717:760	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	10	96	theme	aging	2042:2046	arg1	metabolism					2056:2065	aging protein metabolism	2042:2065	aging protein metabolism	2042:2065	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	2	97	theme	protein	292:298	arg1	blend					300:304	a plant protein blend	284:304	a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine	284:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	6	98	theme	mass	1283:1286	arg1	aging					1251:1255	aging	1251:1255	aging of lean body mass, muscle mass, and muscle functionality	1251:1312	RESULTS There was no difference between protein type on the maintenance during aging of lean body mass, muscle mass, and muscle functionality.
36796433	3	99	theme	protein	747:753	arg1	blend					755:759	plant protein blend	741:759	plant protein blend	741:759	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	2	100	theme	amino	343:347	arg1	acids					349:353	indispensable amino acids	329:353	indispensable amino acids	329:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	4	101	theme	in	995:996	arg1	synthesis					1018:1026	in vivo muscle protein synthesis	995:1026	in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine)	995:1062	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	4	101	theme	in	995:996	arg1	dose					1038:1041	flooding dose	1029:1041	flooding dose of L-[1-13C	1029:1053	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	2	102	theme	leucine	376:382	arg1	balance					318:324	a good balance	311:324	a good balance of indispensable amino acids	311:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	2	102	theme	leucine	376:382	arg1	contents					364:371	high contents	359:371	high contents of leucine, arginine, and cysteine	359:406	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	4	103	theme	muscle	1003:1008	arg1	synthesis					1018:1026	in vivo muscle protein synthesis	995:1026	in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine)	995:1062	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	4	103	theme	muscle	1003:1008	arg1	dose					1038:1041	flooding dose	1029:1041	flooding dose of L-[1-13C	1029:1053	We measured: every 2 mo, body composition and plasma biochemistry; before and after 4 mo, muscle functionality; after 4 mo, in vivo muscle protein synthesis (flooding dose of L-[1-13C]-valine) and muscle, liver, and heart weights.
36796433	2	104	theme	good	313:316	arg1	balance					318:324	a good balance	311:324	a good balance of indispensable amino acids	311:353	OBJECTIVES Our aim was to assess the effect of a plant protein blend with a good balance of indispensable amino acids and high contents of leucine, arginine, and cysteine on the maintenance of muscle protein mass and function during aging in comparison to milk proteins and to determine if this effect varied according to the quality of the background diet.
36796433	0	105	theme	Milk	37:40	arg1	Protein					42:48	Milk Protein	37:48	Milk Protein	37:48	Plant Protein Can Be as Efficient as Milk Protein to Maintain Fat Free Mass in Old Rats, Even When Fat and Sugar Intakes Are High.
36796433	3	106	with	kcal/g	796:801	arg1	starch					809:814	starch	809:814	starch	809:814	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	3	107	theme	energy	766:771	arg1	content					773:779	energy content	766:779	energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose)	766:868	METHODS Old male Wistar rats (n = 96, 18 mo old) were randomly allocated for 4 mo to 1 of 4 diets, differing according to protein source (milk or plant protein blend) and energy content (standard, 3.6 kcal/g, with starch, or high, 4.9 kcal/g, with saturated fat and sucrose).
36796433	10	108	theme	nutritional	1908:1918	arg1	standpoint					1920:1929	the nutritional standpoint	1904:1929	the nutritional standpoint	1904:1929	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	10	109	from	standpoint	1920:1929	arg1	proof					1882:1886	significant proof	1870:1886	significant proof of concept from the nutritional standpoint	1870:1929	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
36796433	1	110	theme	adequate	172:179	arg1	sources					181:187	BACKGROUND Alternative, sustainable, and adequate sources	131:187	BACKGROUND Alternative, sustainable, and adequate sources of protein	131:198	BACKGROUND Alternative, sustainable, and adequate sources of protein must be found to meet global demand.
36796433	7	111	theme	fasting	1457:1463	arg1	glucose					1472:1478	fasting plasma glucose	1457:1478	fasting plasma glucose	1457:1478	The high-energy diet significantly increased body fat (+47%) and heart weight (+8%) compared to the standard energy diet but had no effect on fasting plasma glucose and insulin.
36796433	10	112	theme	protein	2048:2054	arg1	metabolism					2056:2065	aging protein metabolism	2042:2065	aging protein metabolism	2042:2065	However, this rat study offers significant proof of concept from the nutritional standpoint that appropriately blended plant proteins can have high nutritional value even in demanding situations such as aging protein metabolism.
37385329	0	0	theme	barrier	97:103	arg1	function					105:112	gut barrier function	93:112	gut barrier function	93:112	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	5	1	theme	intestinal	826:835	arg1	function					845:852	intestinal barrier function	826:852	intestinal barrier function	826:852	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	3	2	theme	TCC	546:548	arg1	exposure					550:557	TCC exposure	546:557	TCC exposure	546:557	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	0	3	theme	gut	93:95	arg1	function					105:112	gut barrier function	93:112	gut barrier function	93:112	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	3	4	theme	exposure	550:557	arg1	effects					535:541	the deteriorating effects	517:541	the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model	517:619	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	1	5	theme	public	269:274	arg1	issues					283:288	public health issues	269:288	public health issues	269:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	3	6	theme	sequencing	409:418	arg1	examinations					468:479	histopathological and biological examinations	435:479	histopathological and biological examinations	435:479	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	3	6	theme	sequencing	409:418	arg1	metabolomics					421:432	metabolomics	421:432	metabolomics	421:432	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	3	6	theme	sequencing	409:418	arg1	combination					380:390	a combination	378:390	a combination of 16S rRNA gene sequencing	378:418	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	2	7	theme	enterotoxicity	306:319	arg1	mechanisms					321:330	enterotoxicity mechanisms	306:330	enterotoxicity mechanisms of TCC exposure	306:346	Unfortunately, enterotoxicity mechanisms of TCC exposure remain largely unknown.
37385329	8	8	theme	cancer	1441:1446	arg1	development					1408:1418	development	1408:1418	development of IBD or even colon cancer	1408:1446	These findings provided new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer.
37385329	1	9	theme	health	276:281	arg1	issues					283:288	public health issues	269:288	public health issues	269:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	3	10	theme	-induced	592:599	arg1	model					615:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	7	11	theme	mice	1290:1293	arg1	status					1268:1273	colonic inflammatory status	1247:1273	colonic inflammatory status of DSS-treated mice	1247:1293	Consequently, TCC exposure markedly exacerbated colonic inflammatory status of DSS-treated mice by activating NF-κB pathway.
37385329	7	12	theme	NF-κB	1309:1313	arg1	pathway					1315:1321	NF-κB pathway	1309:1321	NF-κB pathway	1309:1321	Consequently, TCC exposure markedly exacerbated colonic inflammatory status of DSS-treated mice by activating NF-κB pathway.
37385329	5	13	theme	expression	953:962	arg1	downregulation					881:894	significant downregulation	869:894	significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin)	869:1022	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	6	14	theme	gut	1029:1031	arg1	composition					1044:1054	The gut microbiota composition	1025:1054	The gut microbiota composition	1025:1054	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	5	15	theme	TCC	795:797	arg1	exposure					799:806	TCC exposure	795:806	TCC exposure	795:806	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	16	theme	proteins	976:983	arg1	number					903:908	the number	899:908	the number of goblet cells	899:924	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	16	theme	proteins	976:983	arg1	expression					953:962	expression	953:962	expression of junction proteins	953:983	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	16	theme	proteins	976:983	arg1	thickness					939:947	mucus layer thickness	927:947	mucus layer thickness	927:947	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	17	theme	significant	869:879	arg1	downregulation					881:894	significant downregulation	869:894	significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin)	869:1022	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	4	18	theme	colitis	693:699	arg1	length					738:743	shortened colon length	722:743	shortened colon length	722:743	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	4	18	theme	colitis	693:699	arg1	histopathology					765:778	altered colonic histopathology	749:778	altered colonic histopathology	749:778	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	4	18	theme	colitis	693:699	arg1	phenotypes					701:710	colitis phenotypes	693:710	colitis phenotypes including shortened colon length and altered colonic histopathology	693:778	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	0	19	theme	microbial	118:126	arg1	community					128:136	microbial community	118:136	microbial community	118:136	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	8	20	theme	IBD	1423:1425	arg1	development					1408:1418	development	1408:1418	development of IBD or even colon cancer	1408:1446	These findings provided new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer.
37385329	0	21	dep	function	105:112	arg1	the					89:91	the	89:91	the	89:91	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	1	22	theme	antibacterial	172:184	arg1	component					186:194	an antibacterial component	169:194	an antibacterial component widely used in personal care products with potential toxicity possessing public health issues	169:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	1	22	theme	antibacterial	172:184	arg1	Triclocarban					147:158	Triclocarban	147:158	Triclocarban (TCC)	147:164	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	3	23	theme	gene	404:407	arg1	sequencing					409:418	16S rRNA gene sequencing	395:418	16S rRNA gene sequencing	395:418	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	5	24	theme	barrier	837:843	arg1	function					845:852	intestinal barrier function	826:852	intestinal barrier function	826:852	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	6	25	theme	tryptophan	1120:1129	arg1	metabolites					1131:1141	tryptophan metabolites	1120:1141	tryptophan metabolites	1120:1141	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	2	26	theme	exposure	339:346	arg1	mechanisms					321:330	enterotoxicity mechanisms	306:330	enterotoxicity mechanisms of TCC exposure	306:346	Unfortunately, enterotoxicity mechanisms of TCC exposure remain largely unknown.
37385329	5	27	theme	layer	933:937	arg1	thickness					939:947	mucus layer thickness	927:947	mucus layer thickness	927:947	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	0	28	theme	Triclocarban	0:11	arg1	exposure					13:20	Triclocarban exposure	0:20	Triclocarban exposure	0:20	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	3	29	from	effects	535:541	arg1	model					615:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	2	30	theme	TCC	335:337	arg1	exposure					339:346	TCC exposure	335:346	TCC exposure	335:346	Unfortunately, enterotoxicity mechanisms of TCC exposure remain largely unknown.
37385329	3	31	theme	biological	457:466	arg1	examinations					468:479	histopathological and biological examinations	435:479	histopathological and biological examinations	435:479	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	3	31	theme	biological	457:466	arg1	combination					380:390	a combination	378:390	a combination of 16S rRNA gene sequencing	378:418	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	0	32	theme	dextran	33:39	arg1	sulfate					41:47	dextran sulfate	33:47	dextran sulfate sodium-induced colitis	33:70	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	6	33	theme	fatty	1096:1100	arg1	acids					1102:1106	short-chain fatty acids	1084:1106	short-chain fatty acids (SCFAs)	1084:1114	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	6	33	theme	fatty	1096:1100	arg1	SCFAs					1109:1113	SCFAs	1109:1113	SCFAs	1109:1113	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	4	34	from	doses	662:666	arg1	exposure					640:647	TCC exposure	636:647	TCC exposure at different doses	636:666	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	4	35	theme	different	652:660	arg1	doses					662:666	different doses	652:666	different doses	652:666	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	3	36	theme	colitis	601:607	arg1	model					615:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	5	37	theme	junction	967:974	arg1	proteins					976:983	junction proteins	967:983	junction proteins	967:983	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	8	38	theme	colon	1435:1439	arg1	cancer					1441:1446	even colon cancer	1430:1446	even colon cancer	1430:1446	These findings provided new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer.
37385329	3	39	theme	rRNA	399:402	arg1	sequencing					409:418	16S rRNA gene sequencing	395:418	16S rRNA gene sequencing	395:418	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	0	40	theme	sodium-induced	49:62	arg1	colitis					64:70	dextran sulfate sodium-induced colitis	33:70	dextran sulfate sodium-induced colitis	33:70	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	6	41	theme	DSS-induced	1173:1183	arg1	colitis					1185:1191	DSS-induced colitis	1173:1191	DSS-induced colitis mice	1173:1196	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	5	42	theme	goblet	913:918	arg1	cells					920:924	goblet cells	913:924	goblet cells	913:924	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	43	theme	thickness	939:947	arg1	downregulation					881:894	significant downregulation	869:894	significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin)	869:1022	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	1	44	theme	personal	211:218	arg1	products					225:232	personal care products	211:232	personal care products with potential toxicity possessing public health issues	211:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	0	45	theme	sulfate	41:47	arg1	colitis					64:70	dextran sulfate sodium-induced colitis	33:70	dextran sulfate sodium-induced colitis	33:70	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	3	46	theme	histopathological	435:451	arg1	examinations					468:479	histopathological and biological examinations	435:479	histopathological and biological examinations	435:479	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	3	46	theme	histopathological	435:451	arg1	combination					380:390	a combination	378:390	a combination of 16S rRNA gene sequencing	378:418	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	3	47	theme	16S	395:397	arg1	sequencing					409:418	16S rRNA gene sequencing	395:418	16S rRNA gene sequencing	395:418	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	5	48	theme	cells	920:924	arg1	number					903:908	the number	899:908	the number of goblet cells	899:924	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	48	theme	cells	920:924	arg1	expression					953:962	expression	953:962	expression of junction proteins	953:983	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	48	theme	cells	920:924	arg1	thickness					939:947	mucus layer thickness	927:947	mucus layer thickness	927:947	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	1	49	theme	care	220:223	arg1	products					225:232	personal care products	211:232	personal care products with potential toxicity possessing public health issues	211:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	6	50	theme	short-chain	1084:1094	arg1	acids					1102:1106	short-chain fatty acids	1084:1106	short-chain fatty acids (SCFAs)	1084:1114	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	6	50	theme	short-chain	1084:1094	arg1	SCFAs					1109:1113	SCFAs	1109:1113	SCFAs	1109:1113	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	3	51	theme	mouse	609:613	arg1	model					615:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	a dextran sulfate sodium (DSS)-induced colitis mouse model	562:619	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	8	52	theme	even	1430:1433	arg1	cancer					1441:1446	even colon cancer	1430:1446	even colon cancer	1430:1446	These findings provided new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer.
37385329	4	53	theme	colonic	757:763	arg1	histopathology					765:778	altered colonic histopathology	749:778	altered colonic histopathology	749:778	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	7	54	theme	inflammatory	1255:1266	arg1	status					1268:1273	colonic inflammatory status	1247:1273	colonic inflammatory status of DSS-treated mice	1247:1293	Consequently, TCC exposure markedly exacerbated colonic inflammatory status of DSS-treated mice by activating NF-κB pathway.
37385329	0	55	from	community	128:136	arg1	mice					141:144	mice	141:144	mice	141:144	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	4	56	theme	altered	749:755	arg1	histopathology					765:778	altered colonic histopathology	749:778	altered colonic histopathology	749:778	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	7	57	theme	TCC	1213:1215	arg1	exposure					1217:1224	TCC exposure	1213:1224	TCC exposure	1213:1224	Consequently, TCC exposure markedly exacerbated colonic inflammatory status of DSS-treated mice by activating NF-κB pathway.
37385329	8	58	theme	new	1348:1350	arg1	evidence					1352:1359	new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer	1348:1446	new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer	1348:1446	These findings provided new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer.
37385329	7	59	theme	DSS-treated	1278:1288	arg1	mice					1290:1293	DSS-treated mice	1278:1293	DSS-treated mice	1278:1293	Consequently, TCC exposure markedly exacerbated colonic inflammatory status of DSS-treated mice by activating NF-κB pathway.
37385329	8	60	theme	environmental	1382:1394	arg1	hazards					1396:1402	an environmental hazards	1379:1402	an environmental hazards for development of IBD or even colon cancer	1379:1446	These findings provided new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer.
37385329	8	60	theme	environmental	1382:1394	arg1	TCC					1366:1368	TCC	1366:1368	TCC	1366:1368	These findings provided new evidence that TCC could be an environmental hazards for development of IBD or even colon cancer.
37385329	3	61	theme	deteriorating	521:533	arg1	effects					535:541	the deteriorating effects	517:541	the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model	517:619	Using a combination of 16S rRNA gene sequencing, metabolomics, histopathological and biological examinations, this study systematically explored the deteriorating effects of TCC exposure on a dextran sulfate sodium (DSS)-induced colitis mouse model.
37385329	6	62	theme	colitis	1185:1191	arg1	mice					1193:1196	DSS-induced colitis mice	1173:1196	DSS-induced colitis mice	1173:1196	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	0	63	from	function	105:112	arg1	mice					141:144	mice	141:144	mice	141:144	Triclocarban exposure aggravates dextran sulfate sodium-induced colitis by deteriorating the gut barrier function and microbial community in mice.
37385329	5	64	dep	number	903:908	arg1	E-cadherin					999:1008	E-cadherin	999:1008	E-cadherin	999:1008	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	64	dep	number	903:908	arg1	ZO-1					993:996	ZO-1	993:996	ZO-1	993:996	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	64	dep	number	903:908	arg1	MUC-2					986:990	MUC-2	986:990	MUC-2	986:990	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	64	dep	number	903:908	arg1	Occludin					1014:1021	Occludin	1014:1021	Occludin	1014:1021	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	5	65	theme	number	903:908	arg1	downregulation					881:894	significant downregulation	869:894	significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin)	869:1022	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	4	66	theme	colon	732:736	arg1	length					738:743	shortened colon length	722:743	shortened colon length	722:743	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	5	67	theme	mucus	927:931	arg1	thickness					939:947	mucus layer thickness	927:947	mucus layer thickness	927:947	Mechanically, TCC exposure further disrupted intestinal barrier function, manifested by significant downregulation of the number of goblet cells, mucus layer thickness and expression of junction proteins (MUC-2, ZO-1, E-cadherin and Occludin).
37385329	1	68	theme	potential	239:247	arg1	toxicity					249:256	potential toxicity	239:256	potential toxicity possessing public health issues	239:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	6	69	theme	microbiota	1033:1042	arg1	composition					1044:1054	The gut microbiota composition	1025:1054	The gut microbiota composition	1025:1054	The gut microbiota composition and its metabolites such as short-chain fatty acids (SCFAs) and tryptophan metabolites were also markedly altered in DSS-induced colitis mice.
37385329	7	70	theme	colonic	1247:1253	arg1	status					1268:1273	colonic inflammatory status	1247:1273	colonic inflammatory status of DSS-treated mice	1247:1293	Consequently, TCC exposure markedly exacerbated colonic inflammatory status of DSS-treated mice by activating NF-κB pathway.
37385329	1	71	contain	possessing	258:267	arg2	issues					283:288	public health issues	269:288	public health issues	269:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	1	71	contain	possessing	258:267	arg1	toxicity					249:256	potential toxicity	239:256	potential toxicity possessing public health issues	239:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	1	72	with	products	225:232	arg1	toxicity					249:256	potential toxicity	239:256	potential toxicity possessing public health issues	239:288	Triclocarban (TCC) is an antibacterial component widely used in personal care products with potential toxicity possessing public health issues.
37385329	4	73	theme	shortened	722:730	arg1	length					738:743	shortened colon length	722:743	shortened colon length	722:743	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
37385329	4	74	theme	TCC	636:638	arg1	exposure					640:647	TCC exposure	636:647	TCC exposure at different doses	636:666	We found that TCC exposure at different doses significantly aggravated colitis phenotypes including shortened colon length and altered colonic histopathology.
35817026	0	0	theme	Hyperactive	102:112	arg1	Microglia					120:128	Hyperactive Human Microglia	102:128	Hyperactive Human Microglia	102:128	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	7	1	from	phosphorylation	1352:1366	arg1	microglia					1404:1412	lipopolysaccharide-treated human microglia	1371:1412	lipopolysaccharide-treated human microglia	1371:1412	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	7	2	theme	excessive	1427:1435	arg1	stress					1447:1452	excessive oxidative stress	1427:1452	excessive oxidative stress	1427:1452	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	5	3	theme	human	981:985	arg1	HMC3					998:1001	HMC3	998:1001	HMC3	998:1001	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	5	3	theme	human	981:985	arg1	microglia					987:995	human microglia	981:995	human microglia (HMC3)	981:1002	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	5	4	theme	reactive	1080:1087	arg1	species					1096:1102	reactive oxygen species	1080:1102	reactive oxygen species (ROS)	1080:1108	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	5	4	theme	reactive	1080:1087	arg1	ROS					1105:1107	ROS	1105:1107	ROS	1105:1107	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	7	5	theme	ERK1/2	1345:1350	arg1	phosphorylation					1352:1366	ERK1/2 phosphorylation	1345:1366	ERK1/2 phosphorylation	1345:1366	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	3	6	theme	Amphiphilic	463:473	arg1	stars					475:479	Amphiphilic stars	463:479	Amphiphilic stars	463:479	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	7	theme	core	597:600	arg1	junction					602:609	the core junction	593:609	the core junction of A and B arms	593:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	8	theme	A	614:614	arg1	arms					622:625	A and B arms	614:625	A and B arms	614:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	0	9	theme	Human	114:118	arg1	Microglia					120:128	Hyperactive Human Microglia	102:128	Hyperactive Human Microglia	102:128	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	4	10	theme	poor	806:809	arg1	solubility					819:828	its poor aqueous solubility	802:828	its poor aqueous solubility	802:828	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	4	11	theme	natural	685:691	arg1	Fisetin					674:680	Fisetin	674:680	Fisetin	674:680	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	4	11	theme	natural	685:691	arg1	flavonoid					693:701	a natural flavonoid	683:701	a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility	683:828	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	1	12	theme	star	140:143	arg1	polymers					145:152	Branched star polymers	131:152	Branched star polymers	131:152	Branched star polymers offer exciting opportunities in enhancing the efficacy of nanocarriers in delivering biologically active lipophilic agents.
35817026	6	13	theme	signaling	1170:1178	arg1	molecules					1180:1188	These signaling molecules	1164:1188	These signaling molecules	1164:1188	These signaling molecules and pathways are implicated in inflammatory processes and cell survival.
35817026	4	14	link	star-derived	872:883	arg1	polymersomes					893:904	miktoarm star-derived aqueous polymersomes	863:904	miktoarm star-derived aqueous polymersomes	863:904	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	3	15	theme	PEG	504:506	arg1	composition					518:528	AB<sub>2</sub> (A = PEG, B = PCL) composition	484:528	AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms	484:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	15	theme	PEG	504:506	arg1	PCL					513:515	A = PEG, B = PCL	500:515	PCL	513:515	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	16	theme	AB<sub>2</sub>	484:497	arg1	composition					518:528	AB<sub>2</sub> (A = PEG, B = PCL) composition	484:528	AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms	484:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	16	theme	AB<sub>2</sub>	484:497	arg1	PCL					513:515	A = PEG, B = PCL	500:515	PCL	513:515	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	2	17	theme	polymeric	311:319	arg1	architecture					321:332	the star polymeric architecture	302:332	the star polymeric architecture	302:332	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	3	18	theme	oxidative	543:551	arg1	stress					553:558	oxidative stress	543:558	oxidative stress	543:558	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	7	19	theme	ERK-mediated	1458:1469	arg1	signaling					1471:1479	ERK-mediated signaling	1458:1479	ERK-mediated signaling	1458:1479	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	0	20	theme	Miktoarm	19:26	arg1	Formulations					42:53	Stimuli-Responsive Miktoarm Polymer-Based Formulations	0:53	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.	0:129	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	5	21	dep	fisetin	1120:1126	arg1	iii					1115:1117	iii	1115:1117	iii	1115:1117	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	2	22	theme	soft	360:363	arg1	nanoparticles					365:377	soft nanoparticles	360:377	soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities	360:460	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	1	23	theme	nanocarriers	212:223	arg1	efficacy					200:207	the efficacy	196:207	the efficacy of nanocarriers in delivering biologically active lipophilic agents	196:275	Branched star polymers offer exciting opportunities in enhancing the efficacy of nanocarriers in delivering biologically active lipophilic agents.
35817026	0	24	theme	Stimuli-Responsive	0:17	arg1	Formulations					42:53	Stimuli-Responsive Miktoarm Polymer-Based Formulations	0:53	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.	0:129	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	4	25	theme	limited	772:778	arg1	value					789:793	limited clinical value	772:793	limited clinical value due to its poor aqueous solubility	772:828	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	3	26	theme	B	620:620	arg1	arms					622:625	A and B arms	614:625	A and B arms	614:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	2	27	theme	chemical	444:451	arg1	entities					453:460	precisely located stimuli-sensitive chemical entities	408:460	precisely located stimuli-sensitive chemical entities	408:460	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	4	28	theme	value	789:793	arg1	Fisetin					674:680	Fisetin	674:680	Fisetin	674:680	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	4	28	theme	value	789:793	arg1	flavonoid					693:701	a natural flavonoid	683:701	a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility	683:828	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	5	29	dep	evaluated	936:944	arg1	ii					1043:1044	ii	1043:1044	ii	1043:1044	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	5	29	dep	evaluated	936:944	arg1	reduces					1055:1061	reduces	1055:1061	reduces the abundance of reactive oxygen species (ROS)	1055:1108	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	7	30	dep	Fisetin	1263:1269	arg1	incorporated					1277:1288	incorporated	1277:1288	incorporated	1277:1288	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	7	30	dep	Fisetin	1263:1269	arg1	nonincorporated					1294:1308	nonincorporated	1294:1308	nonincorporated into polymersomes	1294:1326	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	6	31	theme	cell	1248:1251	arg1	survival					1253:1260	cell survival	1248:1260	cell survival	1248:1260	These signaling molecules and pathways are implicated in inflammatory processes and cell survival.
35817026	0	32	theme	Polymer-Based	28:40	arg1	Formulations					42:53	Stimuli-Responsive Miktoarm Polymer-Based Formulations	0:53	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.	0:129	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	7	33	from	ROS	1337:1339	arg1	microglia					1404:1412	lipopolysaccharide-treated human microglia	1371:1412	lipopolysaccharide-treated human microglia	1371:1412	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	5	34	theme	oxygen	1089:1094	arg1	species					1096:1102	reactive oxygen species	1080:1102	reactive oxygen species (ROS)	1080:1108	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	5	34	theme	oxygen	1089:1094	arg1	ROS					1105:1107	ROS	1105:1107	ROS	1105:1107	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	1	35	theme	Branched	131:138	arg1	polymers					145:152	Branched star polymers	131:152	Branched star polymers	131:152	Branched star polymers offer exciting opportunities in enhancing the efficacy of nanocarriers in delivering biologically active lipophilic agents.
35817026	3	36	theme	responsive	573:582	arg1	units					584:588	responsive units	573:588	responsive units	573:588	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	0	37	theme	Fisetin	59:65	arg1	Delivery					67:74	Fisetin Delivery	59:74	Fisetin Delivery	59:74	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	4	38	theme	anti-inflammatory	719:735	arg1	properties					753:762	remarkable anti-inflammatory and antioxidant properties	708:762	remarkable anti-inflammatory and antioxidant properties	708:762	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	2	39	with	nanoparticles	365:377	arg1	entities					453:460	precisely located stimuli-sensitive chemical entities	408:460	precisely located stimuli-sensitive chemical entities	408:460	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	4	40	theme	star-derived	872:883	arg1	polymersomes					893:904	miktoarm star-derived aqueous polymersomes	863:904	miktoarm star-derived aqueous polymersomes	863:904	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	3	41	theme	=	502:502	arg1	composition					518:528	AB<sub>2</sub> (A = PEG, B = PCL) composition	484:528	AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms	484:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	41	theme	=	502:502	arg1	PCL					513:515	A = PEG, B = PCL	500:515	PCL	513:515	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	1	42	theme	active	252:257	arg1	agents					270:275	biologically active lipophilic agents	239:275	biologically active lipophilic agents	239:275	Branched star polymers offer exciting opportunities in enhancing the efficacy of nanocarriers in delivering biologically active lipophilic agents.
35817026	4	43	theme	antioxidant	741:751	arg1	properties					753:762	remarkable anti-inflammatory and antioxidant properties	708:762	remarkable anti-inflammatory and antioxidant properties	708:762	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	4	44	theme	miktoarm	863:870	arg1	polymersomes					893:904	miktoarm star-derived aqueous polymersomes	863:904	miktoarm star-derived aqueous polymersomes	863:904	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	1	45	theme	lipophilic	259:268	arg1	agents					270:275	biologically active lipophilic agents	239:275	biologically active lipophilic agents	239:275	Branched star polymers offer exciting opportunities in enhancing the efficacy of nanocarriers in delivering biologically active lipophilic agents.
35817026	7	46	theme	human	1398:1402	arg1	microglia					1404:1412	lipopolysaccharide-treated human microglia	1371:1412	lipopolysaccharide-treated human microglia	1371:1412	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	4	47	with	flavonoid	693:701	arg1	properties					753:762	remarkable anti-inflammatory and antioxidant properties	708:762	remarkable anti-inflammatory and antioxidant properties	708:762	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	3	48	theme	arms	622:625	arg1	junction					602:609	the core junction	593:609	the core junction of A and B arms	593:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	2	49	theme	morphology	392:401	arg1	nanoparticles					365:377	soft nanoparticles	360:377	soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities	360:460	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	5	50	theme	ERK1/2	1156:1161	arg1	activation					1142:1151	the activation	1138:1151	the activation of ERK1/2	1138:1161	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	4	51	theme	aqueous	885:891	arg1	polymersomes					893:904	miktoarm star-derived aqueous polymersomes	863:904	miktoarm star-derived aqueous polymersomes	863:904	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	2	52	theme	vesicular	382:390	arg1	morphology					392:401	vesicular morphology	382:401	vesicular morphology	382:401	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	6	53	theme	inflammatory	1221:1232	arg1	processes					1234:1242	inflammatory processes	1221:1242	inflammatory processes	1221:1242	These signaling molecules and pathways are implicated in inflammatory processes and cell survival.
35817026	7	54	theme	lipopolysaccharide-treated	1371:1396	arg1	microglia					1404:1412	lipopolysaccharide-treated human microglia	1371:1412	lipopolysaccharide-treated human microglia	1371:1412	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	4	55	theme	remarkable	708:717	arg1	properties					753:762	remarkable anti-inflammatory and antioxidant properties	708:762	remarkable anti-inflammatory and antioxidant properties	708:762	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	2	56	theme	star	306:309	arg1	architecture					321:332	the star polymeric architecture	302:332	the star polymeric architecture	302:332	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	7	57	theme	oxidative	1437:1445	arg1	stress					1447:1452	excessive oxidative stress	1427:1452	excessive oxidative stress	1427:1452	Fisetin, both incorporated and nonincorporated into polymersomes, reduces ROS and ERK1/2 phosphorylation in lipopolysaccharide-treated human microglia, normalizing excessive oxidative stress and ERK-mediated signaling.
35817026	3	58	theme	composition	518:528	arg1	stars					475:479	Amphiphilic stars	463:479	Amphiphilic stars	463:479	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	59	dep	stress	553:558	arg1	units					584:588	responsive units	573:588	responsive units	573:588	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	4	60	theme	aqueous	811:817	arg1	solubility					819:828	its poor aqueous solubility	802:828	its poor aqueous solubility	802:828	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	5	61	theme	species	1096:1102	arg1	abundance					1067:1075	the abundance	1063:1075	the abundance of reactive oxygen species (ROS)	1063:1108	Polymersomes and fisetin are evaluated separately, and in combination, in human microglia (HMC3), to show if i) polymersomes are toxic; ii) fisetin reduces the abundance of reactive oxygen species (ROS); and iii) fisetin modulates the activation of ERK1/2.
35817026	1	62	theme	exciting	160:167	arg1	opportunities					169:181	exciting opportunities	160:181	exciting opportunities	160:181	Branched star polymers offer exciting opportunities in enhancing the efficacy of nanocarriers in delivering biologically active lipophilic agents.
35817026	4	63	theme	clinical	780:787	arg1	value					789:793	limited clinical value	772:793	limited clinical value due to its poor aqueous solubility	772:828	Fisetin, a natural flavonoid with remarkable anti-inflammatory and antioxidant properties, but of limited clinical value due to its poor aqueous solubility, is physically encapsulated into miktoarm star-derived aqueous polymersomes.
35817026	3	64	theme	=	511:511	arg1	composition					518:528	AB<sub>2</sub> (A = PEG, B = PCL) composition	484:528	AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms	484:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	64	theme	=	511:511	arg1	PCL					513:515	A = PEG, B = PCL	500:515	PCL	513:515	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	65	theme	B	509:509	arg1	composition					518:528	AB<sub>2</sub> (A = PEG, B = PCL) composition	484:528	AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms	484:625	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	3	65	theme	B	509:509	arg1	PCL					513:515	A = PEG, B = PCL	500:515	PCL	513:515	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	2	66	theme	stimuli-sensitive	426:442	arg1	entities					453:460	precisely located stimuli-sensitive chemical entities	408:460	precisely located stimuli-sensitive chemical entities	408:460	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
35817026	0	67	theme	Regulatory	80:89	arg1	Effects					91:97	Regulatory Effects	80:97	Regulatory Effects	80:97	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	0	68	from	Formulations	42:53	arg1	Microglia					120:128	Hyperactive Human Microglia	102:128	Hyperactive Human Microglia	102:128	Stimuli-Responsive Miktoarm Polymer-Based Formulations for Fisetin Delivery and Regulatory Effects in Hyperactive Human Microglia.
35817026	3	69	theme	synthetic	650:658	arg1	articulation					660:671	synthetic articulation	650:671	synthetic articulation	650:671	Amphiphilic stars of AB<sub>2</sub> (A = PEG, B = PCL) composition with/without oxidative stress or reduction responsive units at the core junction of A and B arms, are constructed using synthetic articulation.
35817026	2	70	theme	located	418:424	arg1	entities					453:460	precisely located stimuli-sensitive chemical entities	408:460	precisely located stimuli-sensitive chemical entities	408:460	It is demonstrated that the star polymeric architecture can be leveraged to yield soft nanoparticles of vesicular morphology with precisely located stimuli-sensitive chemical entities.
36106930	9	0	theme	metabolism	1713:1722	arg1	disorders					1724:1732	glucose metabolism disorders	1705:1732	glucose metabolism disorders	1705:1732	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	3	1	from	uptake	520:525	arg1	cells					539:543	IR-HepG2 cells	530:543	IR-HepG2 cells induced by dexamethasone (DEX)	530:574	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	5	2	theme	varied	832:837	arg1	compositions					839:850	the varied compositions	828:850	the varied compositions	828:850	In vitro, the OBG : LA = 1 : 1 group showed the strongest synergistic effects among the varied compositions, outperforming OBG and LA alone.
36106930	6	3	theme	LA	985:986	arg1	groups					1002:1007	the OBG and LA single-dosing groups	973:1007	groups	1002:1007	In vivo, there were more beneficial effects in the OBG : LA = 1 : 1 group compared with the OBG and LA single-dosing groups.
36106930	7	4	theme	blood	1192:1196	arg1	levels					1204:1209	blood lipid levels	1192:1209	blood lipid levels	1192:1209	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	0	5	theme	HepG2	92:96	arg1	cells					98:102	insulin-resistant HepG2 cells	74:102	insulin-resistant HepG2 cells	74:102	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	8	6	theme	liver	1617:1621	arg1	tissues					1623:1629	liver tissues	1617:1629	liver tissues	1617:1629	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	7	theme	3-kinase	1418:1425	arg1	expression					1352:1361	the protein expression	1340:1361	the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT)	1340:1470	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	8	theme	 LA	1309:1311	arg1	1 					1315:1316	 LA = 1 	1309:1316	the OBG : LA = 1 : 1 group	1300:1325	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	7	9	theme	insulin	1163:1169	arg1	sensitivity					1171:1181	insulin sensitivity	1163:1181	insulin sensitivity	1163:1181	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	8	10	theme	insulin	1593:1599	arg1	transduction					1601:1612	insulin transduction	1593:1612	insulin transduction	1593:1612	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	3	11	from	effect	387:392	arg1	uptake					520:525	glucose uptake	512:525	glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX)	512:574	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	8	12	theme	phosphatidylinositol	1397:1416	arg1	PI3K					1428:1431	PI3K	1428:1431	PI3K	1428:1431	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	12	theme	phosphatidylinositol	1397:1416	arg1	3-kinase					1418:1425	phosphatidylinositol 3-kinase	1397:1425	phosphatidylinositol 3-kinase (PI3K)	1397:1432	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	7	13	theme	glucose	1090:1096	arg1	levels					1066:1071	the levels	1062:1071	the levels of fasting blood glucose (FBG) and insulin (INS) in serum	1062:1129	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	3	14	theme	synergistic	620:630	arg1	effects					632:638	synergistic effects	620:638	synergistic effects	620:638	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	1	15	theme	positive	246:253	arg1	effects					255:261	positive effects	246:261	positive effects on diabetes and its complications	246:295	Oat β-glucan (OBG) and L-arabinose (LA) have exhibited positive effects on diabetes and its complications.
36106930	0	16	from	uptake	64:69	arg1	cells					98:102	insulin-resistant HepG2 cells	74:102	insulin-resistant HepG2 cells	74:102	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	8	17	theme	substrate-1	1561:1571	arg1	expression					1507:1516	the protein expression	1495:1516	the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues	1495:1629	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	4	18	theme	composition	717:727	arg1	activity					693:700	the anti-diabetic activity	675:700	the anti-diabetic activity of the optimal composition in db/db mice	675:741	Furthermore, this study evaluated the anti-diabetic activity of the optimal composition in db/db mice.
36106930	7	19	theme	insulin	1108:1114	arg1	levels					1066:1071	the levels	1062:1071	the levels of fasting blood glucose (FBG) and insulin (INS) in serum	1062:1129	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	9	20	dep	OBG 	1660:1663	arg1	 1					1674:1675	 1	1674:1675	 1	1674:1675	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	9	20	dep	OBG 	1660:1663	arg1	 LA					1665:1667	 LA	1665:1667	OBG : LA = 1 : 1	1660:1675	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	7	21	theme	fasting	1076:1082	arg1	FBG					1099:1101	FBG	1099:1101	FBG	1099:1101	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	7	21	theme	fasting	1076:1082	arg1	glucose					1090:1096	fasting blood glucose	1076:1096	fasting blood glucose (FBG)	1076:1102	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	2	22	theme	synergistic	347:357	arg1	effect					359:364	a synergistic effect	345:364	a synergistic effect	345:364	However, it is unclear whether OBG and LA have a synergistic effect.
36106930	3	23	theme	compositions	406:417	arg1	effect					387:392	the effect	383:392	the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX)	383:574	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	0	24	theme	anti-diabetic	114:126	arg1	activity					128:135	anti-diabetic activity	114:135	anti-diabetic activity	114:135	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	9	25	theme	insulin	1749:1755	arg1	resistance					1757:1766	insulin resistance	1749:1766	insulin resistance	1749:1766	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	7	26	theme	blood	1084:1088	arg1	FBG					1099:1101	FBG	1099:1101	FBG	1099:1101	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	7	26	theme	blood	1084:1088	arg1	glucose					1090:1096	fasting blood glucose	1076:1096	fasting blood glucose (FBG)	1076:1102	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	8	27	theme	 1	1318:1319	arg1	group					1321:1325	 1 group	1318:1325	the OBG : LA = 1 : 1 group	1300:1325	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	2	28	contain	have	340:343	arg1	LA					337:338	LA	337:338	LA	337:338	However, it is unclear whether OBG and LA have a synergistic effect.
36106930	2	28	contain	have	340:343	arg1	OBG					329:331	OBG	329:331	OBG	329:331	However, it is unclear whether OBG and LA have a synergistic effect.
36106930	2	28	contain	have	340:343	arg2	effect					359:364	a synergistic effect	345:364	a synergistic effect	345:364	However, it is unclear whether OBG and LA have a synergistic effect.
36106930	4	29	theme	db/db	732:736	arg1	mice					738:741	db/db mice	732:741	db/db mice	732:741	Furthermore, this study evaluated the anti-diabetic activity of the optimal composition in db/db mice.
36106930	8	30	theme	phospho-phosphorylated	1521:1542	arg1	p-IRS1					1574:1579	p-IRS1	1574:1579	p-IRS1	1574:1579	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	30	theme	phospho-phosphorylated	1521:1542	arg1	substrate-1					1561:1571	phospho-phosphorylated insulin receptor substrate-1	1521:1571	phospho-phosphorylated insulin receptor substrate-1 (p-IRS1)	1521:1580	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	1	31	theme	Oat	191:193	arg1	OBG					205:207	OBG	205:207	OBG	205:207	Oat β-glucan (OBG) and L-arabinose (LA) have exhibited positive effects on diabetes and its complications.
36106930	1	31	theme	Oat	191:193	arg1	β-glucan					195:202	Oat β-glucan	191:202	Oat β-glucan (OBG)	191:208	Oat β-glucan (OBG) and L-arabinose (LA) have exhibited positive effects on diabetes and its complications.
36106930	0	32	theme	Oat	0:2	arg1	β-glucan					4:11	Oat β-glucan	0:11	Oat β-glucan	0:11	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	9	33	from	pathway	1794:1800	arg1	liver					1809:1813	the liver	1805:1813	the liver	1805:1813	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	8	34	theme	glucose	1366:1372	arg1	GLUT4					1389:1393	GLUT4	1389:1393	GLUT4	1389:1393	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	34	theme	glucose	1366:1372	arg1	transporter-4					1374:1386	glucose transporter-4	1366:1386	glucose transporter-4 (GLUT4)	1366:1394	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	35	theme	insulin	1544:1550	arg1	p-IRS1					1574:1579	p-IRS1	1574:1579	p-IRS1	1574:1579	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	35	theme	insulin	1544:1550	arg1	substrate-1					1561:1571	phospho-phosphorylated insulin receptor substrate-1	1521:1571	phospho-phosphorylated insulin receptor substrate-1 (p-IRS1)	1521:1580	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	7	36	theme	=	1019:1019	arg1	1 					1021:1022	 LA = 1 	1015:1022	OBG : LA = 1 : 1 supplementation	1010:1041	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	3	37	theme	variable	397:404	arg1	compositions					406:417	variable compositions	397:417	variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1)	397:507	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	38	theme	glucose	512:518	arg1	uptake					520:525	glucose uptake	512:525	glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX)	512:574	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	8	39	theme	protein	1499:1505	arg1	expression					1507:1516	the protein expression	1495:1516	the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues	1495:1629	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	7	40	dep	OBG 	1010:1013	arg1	1 					1021:1022	 LA = 1 	1015:1022	OBG : LA = 1 : 1 supplementation	1010:1041	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	7	40	dep	OBG 	1010:1013	arg1	supplementation					1027:1041	 1 supplementation	1024:1041	OBG : LA = 1 : 1 supplementation	1010:1041	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	3	41	theme	 LA	425:427	arg1	1 					431:432	 LA = 1 	425:432	 LA = 1 	425:432	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	7	42	theme	lipid	1198:1202	arg1	levels					1204:1209	blood lipid levels	1192:1209	blood lipid levels	1192:1209	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	1	43	from	effects	255:261	arg1	diabetes					266:273	diabetes	266:273	diabetes	266:273	Oat β-glucan (OBG) and L-arabinose (LA) have exhibited positive effects on diabetes and its complications.
36106930	1	43	from	effects	255:261	arg1	complications					283:295	its complications	279:295	its complications	279:295	Oat β-glucan (OBG) and L-arabinose (LA) have exhibited positive effects on diabetes and its complications.
36106930	4	44	from	activity	693:700	arg1	mice					738:741	db/db mice	732:741	db/db mice	732:741	Furthermore, this study evaluated the anti-diabetic activity of the optimal composition in db/db mice.
36106930	8	45	theme	kinase	1455:1460	arg1	p-AKT					1465:1469	p-AKT	1465:1469	p-AKT	1465:1469	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	45	theme	kinase	1455:1460	arg1	B					1462:1462	phospho-protein kinase B	1439:1462	phospho-protein kinase B (p-AKT)	1439:1470	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	46	theme	protein	1344:1350	arg1	expression					1352:1361	the protein expression	1340:1361	the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT)	1340:1470	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	3	47	theme	=	429:429	arg1	1 					431:432	 LA = 1 	425:432	 LA = 1 	425:432	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	8	48	theme	=	1313:1313	arg1	1 					1315:1316	 LA = 1 	1309:1316	the OBG : LA = 1 : 1 group	1300:1325	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	9	49	theme	PI3K/AKT	1785:1792	arg1	pathway					1794:1800	the PI3K/AKT pathway	1781:1800	the PI3K/AKT pathway in the liver	1781:1813	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	3	50	theme	IR-HepG2	530:537	arg1	cells					539:543	IR-HepG2 cells	530:543	IR-HepG2 cells induced by dexamethasone (DEX)	530:574	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	4	51	theme	anti-diabetic	679:691	arg1	activity					693:700	the anti-diabetic activity	675:700	the anti-diabetic activity of the optimal composition in db/db mice	675:741	Furthermore, this study evaluated the anti-diabetic activity of the optimal composition in db/db mice.
36106930	5	52	theme	strongest	792:800	arg1	effects					814:820	the strongest synergistic effects	788:820	the strongest synergistic effects	788:820	In vitro, the OBG : LA = 1 : 1 group showed the strongest synergistic effects among the varied compositions, outperforming OBG and LA alone.
36106930	4	53	theme	optimal	709:715	arg1	composition					717:727	the optimal composition	705:727	the optimal composition	705:727	Furthermore, this study evaluated the anti-diabetic activity of the optimal composition in db/db mice.
36106930	8	54	dep	OBG 	1304:1307	arg1	1 					1315:1316	 LA = 1 	1309:1316	the OBG : LA = 1 : 1 group	1300:1325	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	54	dep	OBG 	1304:1307	arg1	group					1321:1325	 1 group	1318:1325	the OBG : LA = 1 : 1 group	1300:1325	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	0	55	theme	pathway	168:174	arg1	activation					141:150	activation	141:150	activation of the PI3K/AKT pathway in db/db mice	141:188	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	5	56	theme	synergistic	802:812	arg1	effects					814:820	the strongest synergistic effects	788:820	the strongest synergistic effects	788:820	In vitro, the OBG : LA = 1 : 1 group showed the strongest synergistic effects among the varied compositions, outperforming OBG and LA alone.
36106930	3	57	dep	compositions	406:417	arg1	1 					445:446	1 	445:446	1 	445:446	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	57	dep	compositions	406:417	arg1	6 					487:488	6 	487:488	6 	487:488	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	57	dep	compositions	406:417	arg1	OBG 					420:423	OBG 	420:423	OBG 	420:423	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	8	58	theme	phospho-protein	1439:1453	arg1	p-AKT					1465:1469	p-AKT	1465:1469	p-AKT	1465:1469	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	58	theme	phospho-protein	1439:1453	arg1	B					1462:1462	phospho-protein kinase B	1439:1462	phospho-protein kinase B (p-AKT)	1439:1470	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	0	59	theme	glucose	56:62	arg1	uptake					64:69	glucose uptake	56:69	glucose uptake in insulin-resistant HepG2 cells	56:102	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	7	60	theme	liver	1224:1228	arg1	accumulation					1236:1247	liver lipid accumulation	1224:1247	liver lipid accumulation	1224:1247	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	0	61	theme	PI3K/AKT	159:166	arg1	pathway					168:174	the PI3K/AKT pathway	155:174	the PI3K/AKT pathway	155:174	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	7	62	theme	 1	1024:1025	arg1	supplementation					1027:1041	 1 supplementation	1024:1041	OBG : LA = 1 : 1 supplementation	1010:1041	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	8	63	theme	transporter-4	1374:1386	arg1	expression					1352:1361	the protein expression	1340:1361	the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT)	1340:1470	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	9	64	theme	=	1669:1669	arg1	 LA					1665:1667	 LA	1665:1667	OBG : LA = 1 : 1	1660:1675	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	3	65	theme	optimal	592:598	arg1	composition					600:610	the optimal composition	588:610	the optimal composition showing synergistic effects	588:638	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	7	66	theme	lipid	1230:1234	arg1	accumulation					1236:1247	liver lipid accumulation	1224:1247	liver lipid accumulation	1224:1247	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	0	67	theme	db/db	179:183	arg1	mice					185:188	db/db mice	179:188	db/db mice	179:188	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	7	68	theme	glucose	1141:1147	arg1	tolerance					1149:1157	glucose tolerance	1141:1157	glucose tolerance	1141:1157	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	3	69	dep	OBG 	420:423	arg1	 1					476:477	 1	476:477	 1	476:477	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	10 					501:503	10 	501:503	10 	501:503	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	 6					454:455	 6	454:455	 6	454:455	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	1 					431:432	 LA = 1 	425:432	 LA = 1 	425:432	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	 1					434:435	 1	434:435	 1	434:435	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	 1					505:506	 1	505:506	 1	505:506	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	2 					473:474	2 	473:474	2 	473:474	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	 4,1 					448:452	 4,1 	448:452	 4,1 	448:452	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	 8					461:462	 8	461:462	 8	461:462	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	3	69	dep	OBG 	420:423	arg1	 1					490:491	 1	490:491	 1	490:491	We investigated the effect of variable compositions (OBG : LA = 1 : 1, 1 : 2, 1 : 4,1 : 6, 1 : 8, 1 : 10, 2 : 1, 4 : 1, 6 : 1, 8 : 1, 10 : 1) on glucose uptake in IR-HepG2 cells induced by dexamethasone (DEX) to find out the optimal composition showing synergistic effects.
36106930	5	70	dep	OBG 	758:761	arg1	group					775:779	 1 group	772:779	the OBG : LA = 1 : 1 group	754:779	In vitro, the OBG : LA = 1 : 1 group showed the strongest synergistic effects among the varied compositions, outperforming OBG and LA alone.
36106930	5	70	dep	OBG 	758:761	arg1	=					767:767	=	767:767	=	767:767	In vitro, the OBG : LA = 1 : 1 group showed the strongest synergistic effects among the varied compositions, outperforming OBG and LA alone.
36106930	8	71	theme	blot	1272:1275	arg1	results					1277:1283	the western blot results	1260:1283	the western blot results	1260:1283	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	6	72	theme	single-dosing	988:1000	arg1	groups					1002:1007	the OBG and LA single-dosing groups	973:1007	groups	1002:1007	In vivo, there were more beneficial effects in the OBG : LA = 1 : 1 group compared with the OBG and LA single-dosing groups.
36106930	8	73	theme	receptor	1552:1559	arg1	p-IRS1					1574:1579	p-IRS1	1574:1579	p-IRS1	1574:1579	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	73	theme	receptor	1552:1559	arg1	substrate-1					1561:1571	phospho-phosphorylated insulin receptor substrate-1	1521:1571	phospho-phosphorylated insulin receptor substrate-1 (p-IRS1)	1521:1580	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	5	74	theme	 1	772:773	arg1	group					775:779	 1 group	772:779	the OBG : LA = 1 : 1 group	754:779	In vitro, the OBG : LA = 1 : 1 group showed the strongest synergistic effects among the varied compositions, outperforming OBG and LA alone.
36106930	6	75	theme	beneficial	910:919	arg1	effects					921:927	more beneficial effects	905:927	more beneficial effects	905:927	In vivo, there were more beneficial effects in the OBG : LA = 1 : 1 group compared with the OBG and LA single-dosing groups.
36106930	7	76	from	levels	1066:1071	arg1	serum					1125:1129	serum	1125:1129	serum	1125:1129	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	9	77	theme	glucose	1705:1711	arg1	metabolism					1713:1722	glucose metabolism	1705:1722	glucose metabolism disorders	1705:1732	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36106930	0	78	theme	insulin-resistant	74:90	arg1	cells					98:102	insulin-resistant HepG2 cells	74:102	insulin-resistant HepG2 cells	74:102	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	6	79	theme	more	905:908	arg1	effects					921:927	more beneficial effects	905:927	more beneficial effects	905:927	In vivo, there were more beneficial effects in the OBG : LA = 1 : 1 group compared with the OBG and LA single-dosing groups.
36106930	0	80	from	activation	141:150	arg1	mice					185:188	db/db mice	179:188	db/db mice	179:188	Oat β-glucan and L-arabinose synergistically ameliorate glucose uptake in insulin-resistant HepG2 cells and exert anti-diabetic activity via activation of the PI3K/AKT pathway in db/db mice.
36106930	8	81	theme	western	1264:1270	arg1	results					1277:1283	the western blot results	1260:1283	the western blot results	1260:1283	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	8	82	theme	B	1462:1462	arg1	expression					1352:1361	the protein expression	1340:1361	the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT)	1340:1470	Moreover, the western blot results indicated that the OBG : LA = 1 : 1 group up-regulated the protein expression of glucose transporter-4 (GLUT4), phosphatidylinositol 3-kinase (PI3K), and phospho-protein kinase B (p-AKT), while down-regulating the protein expression of phospho-phosphorylated insulin receptor substrate-1 (p-IRS1) to enhance insulin transduction in liver tissues.
36106930	7	83	theme	 LA	1015:1017	arg1	1 					1021:1022	 LA = 1 	1015:1022	OBG : LA = 1 : 1 supplementation	1010:1041	OBG : LA = 1 : 1 supplementation markedly decreased the levels of fasting blood glucose (FBG) and insulin (INS) in serum, improved glucose tolerance and insulin sensitivity, lowered blood lipid levels, and reduced liver lipid accumulation.
36106930	9	84	dep	=	1669:1669	arg1	1 					1671:1672	1 	1671:1672	1 	1671:1672	These findings suggest that OBG : LA = 1 : 1 synergistically ameliorated glucose metabolism disorders and alleviated insulin resistance by promoting the PI3K/AKT pathway in the liver.
36296712	6	0	theme	±	1052:1052	arg1	µg/mL					1059:1063	23.12 ± 1.28 µg/mL	1046:1063	23.12 ± 1.28 µg/mL	1046:1063	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	0	theme	±	1052:1052	arg1	scavenging					1034:1043	in vitro DPPH scavenging	1020:1043	in vitro DPPH scavenging (23.12 ± 1.28 µg/mL)	1020:1064	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	9	1	theme	MDAMB231	1665:1672	arg1	IC50					1674:1677	MDAMB231 IC50	1665:1677	MDAMB231 IC50 = 55.37 ± 2.11µg/mL	1665:1697	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	1	2	theme	ancient	267:273	arg1	times					275:279	ancient times	267:279	ancient times	267:279	Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times.
36296712	6	3	theme	H2O2	1105:1108	arg1	µg/mL					1135:1139	12.92 ± 1.05 µg/mL	1122:1139	12.92 ± 1.05 µg/mL	1122:1139	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	3	theme	H2O2	1105:1108	arg1	scavenging					1110:1119	H2O2 scavenging	1105:1119	H2O2 scavenging (12.92 ± 1.05 µg/mL)	1105:1140	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	9	4	theme	=	1679:1679	arg1	IC50					1674:1677	MDAMB231 IC50	1665:1677	MDAMB231 IC50 = 55.37 ± 2.11µg/mL	1665:1697	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	6	5	theme	±	1158:1158	arg1	µg/mL					1165:1169	5.42 ± 0.19 µg/mL	1153:1169	5.42 ± 0.19 µg/mL	1153:1169	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	5	theme	±	1158:1158	arg1	FRAP					1147:1150	FRAP	1147:1150	FRAP (5.42 ± 0.19 µg/mL)	1147:1170	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	7	6	theme	LPS-stimulated	1297:1310	arg1	production					1312:1321	the LPS-stimulated production	1293:1321	the LPS-stimulated production of cytokines	1293:1334	The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (p &lt; 0.05).
36296712	10	7	theme	LCMS	1866:1869	arg1	analysis					1871:1878	the LCMS analysis	1862:1878	the LCMS analysis	1862:1878	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	9	8	dep	IC50	1644:1647	arg1	IC50					1674:1677	MDAMB231 IC50	1665:1677	MDAMB231 IC50 = 55.37 ± 2.11µg/mL	1665:1697	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	2	9	theme	Various	282:288	arg1	medicines					302:310	traditional medicines	290:310	traditional medicines	290:310	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	8	10	theme	sorbitol	1527:1534	arg1	µg/mL					1564:1568	29.57 ± 1.46 µg/mL	1551:1568	29.57 ± 1.46 µg/mL	1551:1568	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	8	10	theme	sorbitol	1527:1534	arg1	dehydrogenase					1536:1548	sorbitol dehydrogenase	1527:1548	sorbitol dehydrogenase (29.57 ± 1.46 µg/mL)	1527:1569	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	4	11	dep	India	748:752	arg1	Kerala					740:745	Kerala	740:745	Kerala	740:745	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	9	12	theme	colon	1704:1708	arg1	cells					1738:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	0	13	theme	nilagirica	129:138	arg1	Activities					105:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities	52:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke)	52:152	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	2	14	theme	folk	316:319	arg1	systems					321:327	folk systems	316:327	folk systems	316:327	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	8	15	theme	±	1557:1557	arg1	µg/mL					1564:1568	29.57 ± 1.46 µg/mL	1551:1568	29.57 ± 1.46 µg/mL	1551:1568	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	8	15	theme	±	1557:1557	arg1	dehydrogenase					1536:1548	sorbitol dehydrogenase	1527:1548	sorbitol dehydrogenase (29.57 ± 1.46 µg/mL)	1527:1569	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	3	16	dep	their	653:657	arg1	uses					659:662	uses	659:662	uses	659:662	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	1	17	theme	various	222:228	arg1	diseases					253:260	various infectious and chronic diseases	222:260	various infectious and chronic diseases from ancient times	222:279	Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times.
36296712	10	18	theme	constituents	1923:1934	arg1	presence					1893:1900	the presence	1889:1900	the presence of various bioactive constituents including artemisinin	1889:1956	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	6	19	theme	±	1128:1128	arg1	µg/mL					1135:1139	12.92 ± 1.05 µg/mL	1122:1139	12.92 ± 1.05 µg/mL	1122:1139	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	19	theme	±	1128:1128	arg1	scavenging					1110:1119	H2O2 scavenging	1105:1119	H2O2 scavenging (12.92 ± 1.05 µg/mL)	1105:1140	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	10	20	theme	various	1905:1911	arg1	constituents					1923:1934	various bioactive constituents	1905:1934	various bioactive constituents including artemisinin	1905:1956	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	10	20	theme	various	1905:1911	arg1	artemisinin					1946:1956	artemisinin	1946:1956	artemisinin	1946:1956	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	10	21	theme	phytochemical	1764:1776	arg1	screening					1778:1786	the phytochemical screening	1760:1786	the phytochemical screening	1760:1786	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	10	22	theme	flavonoids	1832:1841	arg1	level					1807:1811	a higher level	1798:1811	a higher level of polyphenols and flavonoids in the extract	1798:1856	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	6	23	theme	in	1020:1021	arg1	µg/mL					1059:1063	23.12 ± 1.28 µg/mL	1046:1063	23.12 ± 1.28 µg/mL	1046:1063	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	23	theme	in	1020:1021	arg1	scavenging					1034:1043	in vitro DPPH scavenging	1020:1043	in vitro DPPH scavenging (23.12 ± 1.28 µg/mL)	1020:1064	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	10	24	theme	higher	1800:1805	arg1	level					1807:1811	a higher level	1798:1811	a higher level of polyphenols and flavonoids in the extract	1798:1856	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	0	25	theme	Enzyme-Inhibitory	87:103	arg1	Activities					105:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities	52:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke)	52:152	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	9	26	dep	=	1649:1649	arg1	±					1657:1657	41.79 ± 1.07	1651:1662	41.79 ± 1.07	1651:1662	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	2	27	theme	modern	423:428	arg1	medicine					430:437	modern medicine	423:437	modern medicine	423:437	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	6	28	dep	in	1020:1021	arg1	vitro					1023:1027	vitro	1023:1027	vitro	1023:1027	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	2	29	theme	drug	403:406	arg1	candidates					408:417	drug candidates	403:417	drug candidates for modern medicine	403:437	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	0	30	theme	Phytochemical	0:12	arg1	Composition					14:24	Phytochemical Composition	0:24	Phytochemical Composition	0:24	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	9	31	from	cells	1738:1742	arg1	inhibition					1600:1609	significant inhibition	1588:1609	significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1588:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	9	32	theme	proliferation	1614:1626	arg1	inhibition					1600:1609	significant inhibition	1588:1609	significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1588:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	5	33	theme	anticancer	909:918	arg1	activities					920:929	anticancer activities	909:929	anticancer activities	909:929	In the current study, the phytochemical composition and the antioxidant, enzyme-inhibitory, anti-inflammatory, and anticancer activities were examined.
36296712	9	34	theme	breast	1631:1636	arg1	cells					1738:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	9	35	theme	MCF7	1639:1642	arg1	IC50					1644:1647	MCF7 IC50	1639:1647	MCF7 IC50 = 41.79 ± 1.07	1639:1662	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	8	36	theme	±	1433:1433	arg1	µg/mL					1440:1444	38.42 ± 2.71 µg/mL	1427:1444	38.42 ± 2.71 µg/mL	1427:1444	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	8	36	theme	±	1433:1433	arg1	α-amylase					1416:1424	α-amylase	1416:1424	α-amylase (38.42 ± 2.71 µg/mL)	1416:1445	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	2	37	theme	plant	363:367	arg1	sources					392:398	sources	392:398	sources of drug candidates for modern medicine	392:437	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	37	theme	plant	363:367	arg1	products					369:376	plant products	363:376	plant products	363:376	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	37	theme	plant	363:367	arg1	plants					352:357	numerous plants	343:357	numerous plants	343:357	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	6	38	theme	ABTS	1067:1070	arg1	µg/mL					1097:1101	27.44 ± 1.88 µg/mL	1084:1101	27.44 ± 1.88 µg/mL	1084:1101	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	38	theme	ABTS	1067:1070	arg1	scavenging					1072:1081	ABTS scavenging	1067:1081	ABTS scavenging (27.44 ± 1.88 µg/mL)	1067:1102	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	9	39	theme	=	1649:1649	arg1	IC50					1644:1647	MCF7 IC50	1639:1647	MCF7 IC50 = 41.79 ± 1.07	1639:1662	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	3	40	theme	family	479:484	arg1	Artemisia					440:448	Artemisia	440:448	Artemisia	440:448	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	3	40	theme	family	479:484	arg1	genus					455:459	a genus	453:459	a genus of the Asteraceae family with more than 500 species	453:511	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	1	41	theme	infectious	230:239	arg1	diseases					253:260	various infectious and chronic diseases	222:260	various infectious and chronic diseases from ancient times	222:279	Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times.
36296712	8	42	theme	reductase	1491:1499	arg1	activities					1402:1411	the activities	1398:1411	the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL)	1398:1569	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	1	43	theme	chronic	245:251	arg1	diseases					253:260	various infectious and chronic diseases	222:260	various infectious and chronic diseases from ancient times	222:279	Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times.
36296712	6	44	theme	0.19	1160:1163	arg1	µg/mL					1165:1169	5.42 ± 0.19 µg/mL	1153:1169	5.42 ± 0.19 µg/mL	1153:1169	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	44	theme	0.19	1160:1163	arg1	FRAP					1147:1150	FRAP	1147:1150	FRAP (5.42 ± 0.19 µg/mL)	1147:1170	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	9	45	from	proliferation	1614:1626	arg1	cells					1738:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	7	46	theme	cytokines	1326:1334	arg1	production					1312:1321	the LPS-stimulated production	1293:1321	the LPS-stimulated production of cytokines	1293:1334	The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (p &lt; 0.05).
36296712	3	47	with	family	479:484	arg1	species					505:511	more than 500 species	491:511	more than 500 species	491:511	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	2	48	theme	traditional	290:300	arg1	medicines					302:310	traditional medicines	290:310	traditional medicines	290:310	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	5	49	theme	current	801:807	arg1	study					809:813	the current study	797:813	the current study	797:813	In the current study, the phytochemical composition and the antioxidant, enzyme-inhibitory, anti-inflammatory, and anticancer activities were examined.
36296712	9	50	from	inhibition	1600:1609	arg1	cells					1738:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	4	51	theme	genus	787:791	arg1	member					773:778	a prominent member	761:778	a prominent member of the genus	761:791	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	9	52	dep	breast	1631:1636	arg1	IC50					1644:1647	MCF7 IC50	1639:1647	MCF7 IC50 = 41.79 ± 1.07	1639:1662	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	6	53	theme	±	1090:1090	arg1	µg/mL					1097:1101	27.44 ± 1.88 µg/mL	1084:1101	27.44 ± 1.88 µg/mL	1084:1101	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	53	theme	±	1090:1090	arg1	scavenging					1072:1081	ABTS scavenging	1067:1081	ABTS scavenging (27.44 ± 1.88 µg/mL)	1067:1102	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	1	54	from	times	275:279	arg1	diseases					253:260	various infectious and chronic diseases	222:260	various infectious and chronic diseases from ancient times	222:279	Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times.
36296712	3	55	theme	species	537:543	arg1	many					523:526	many	523:526	many	523:526	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	3	55	theme	species	537:543	arg1	species					537:543	these species	531:543	these species	531:543	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	8	56	theme	dehydrogenase	1536:1548	arg1	activities					1402:1411	the activities	1398:1411	the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL)	1398:1569	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	1	57	theme	therapeutic	189:199	arg1	applications					201:212	therapeutic applications	189:212	therapeutic applications against various infectious and chronic diseases from ancient times	189:279	Plants have been employed in therapeutic applications against various infectious and chronic diseases from ancient times.
36296712	3	58	theme	biological	573:582	arg1	efficacy					584:591	their biological efficacy	567:591	their biological efficacy	567:591	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	9	59	theme	±	1717:1717	arg1	µg/mL					1724:1728	49.57 ± 1.46 µg/mL	1711:1728	49.57 ± 1.46 µg/mL	1711:1728	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	9	59	theme	±	1717:1717	arg1	breast					1631:1636	breast	1631:1636	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL)	1631:1698	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	10	60	theme	bioactive	1913:1921	arg1	constituents					1923:1934	various bioactive constituents	1905:1934	various bioactive constituents including artemisinin	1905:1956	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	10	60	theme	bioactive	1913:1921	arg1	artemisinin					1946:1956	artemisinin	1946:1956	artemisinin	1946:1956	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	3	61	theme	several	598:604	arg1	others					606:611	several others	598:611	several others	598:611	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	0	62	dep	Pamp	154:157	arg1	Antioxidant					39:49	In Vitro Antioxidant	30:49	In Vitro Antioxidant	30:49	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	0	62	dep	Pamp	154:157	arg1	Composition					14:24	Phytochemical Composition	0:24	Phytochemical Composition	0:24	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	0	62	dep	Pamp	154:157	arg1	Activities					105:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities	52:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke)	52:152	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	9	63	theme	cancer	1731:1736	arg1	cells					1738:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1631:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	0	64	theme	Anticancer	71:80	arg1	Activities					105:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities	52:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke)	52:152	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	5	65	theme	phytochemical	820:832	arg1	composition					834:844	the phytochemical composition	816:844	the phytochemical composition	816:844	In the current study, the phytochemical composition and the antioxidant, enzyme-inhibitory, anti-inflammatory, and anticancer activities were examined.
36296712	6	66	theme	ethanol	977:983	arg1	extract					985:991	the ethanol extract	973:991	the ethanol extract of A. nilagirica	973:1008	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	7	67	theme	Raw	1226:1228	arg1	macrophages					1236:1246	the Raw 264.7 macrophages	1222:1246	the Raw 264.7 macrophages	1222:1246	The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (p &lt; 0.05).
36296712	4	68	theme	Artemisia	665:673	arg1	nilagirica					675:684	Artemisia nilagirica	665:684	Artemisia nilagirica	665:684	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	4	68	theme	Artemisia	665:673	arg1	plant					691:695	a plant	689:695	a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus	689:791	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	7	69	dep	reduced	1285:1291	arg1	p					1337:1337	p	1337:1337	p &lt; 0.05	1337:1347	The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (p &lt; 0.05).
36296712	10	70	from	level	1807:1811	arg1	extract					1850:1856	the extract	1846:1856	the extract	1846:1856	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	10	71	theme	screening	1778:1786	arg1	results					1749:1755	The results	1745:1755	The results of the phytochemical screening	1745:1786	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	7	72	with	pretreatment	1255:1266	arg1	extract					1277:1283	the extract	1273:1283	the extract	1273:1283	The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (p &lt; 0.05).
36296712	9	73	theme	significant	1588:1598	arg1	inhibition					1600:1609	significant inhibition	1588:1609	significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells	1588:1742	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	9	74	dep	=	1679:1679	arg1	2.11µg/mL					1689:1697	55.37 ± 2.11µg/mL	1681:1697	55.37 ± 2.11µg/mL	1681:1697	It also induced significant inhibition of proliferation in breast (MCF7 IC50 = 41.79 ± 1.07, MDAMB231 IC50 = 55.37 ± 2.11µg/mL) and colon (49.57 ± 1.46 µg/mL) cancer cells.
36296712	6	75	theme	DPPH	1029:1032	arg1	µg/mL					1059:1063	23.12 ± 1.28 µg/mL	1046:1063	23.12 ± 1.28 µg/mL	1046:1063	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	6	75	theme	DPPH	1029:1032	arg1	scavenging					1034:1043	in vitro DPPH scavenging	1020:1043	in vitro DPPH scavenging (23.12 ± 1.28 µg/mL)	1020:1064	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	8	76	theme	α-amylase	1416:1424	arg1	activities					1402:1411	the activities	1398:1411	the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL)	1398:1569	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	0	77	theme	In	30:31	arg1	Antioxidant					39:49	In Vitro Antioxidant	30:49	In Vitro Antioxidant	30:49	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	7	78	theme	anti-inflammatory	1177:1193	arg1	effect					1195:1200	The anti-inflammatory effect	1173:1200	The anti-inflammatory effect	1173:1200	The anti-inflammatory effect was also noticed in the Raw 264.7 macrophages, where pretreatment with the extract reduced the LPS-stimulated production of cytokines (p &lt; 0.05).
36296712	8	79	theme	38.42	1427:1431	arg1	µg/mL					1440:1444	38.42 ± 2.71 µg/mL	1427:1444	38.42 ± 2.71 µg/mL	1427:1444	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	8	79	theme	38.42	1427:1431	arg1	α-amylase					1416:1424	α-amylase	1416:1424	α-amylase (38.42 ± 2.71 µg/mL)	1416:1445	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	3	80	theme	scientific	625:634	arg1	explanations					636:647	scientific explanations	625:647	scientific explanations for their uses	625:662	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	3	81	theme	Asteraceae	468:477	arg1	family					479:484	the Asteraceae family	464:484	the Asteraceae family with more than 500 species	464:511	Artemisia is a genus of the Asteraceae family with more than 500 species; however, many of these species are less explored for their biological efficacy, and several others are lacking scientific explanations for their uses.
36296712	6	82	theme	nilagirica	999:1008	arg1	extract					985:991	the ethanol extract	973:991	the ethanol extract of A. nilagirica	973:1008	The results indicated that the ethanol extract of A. nilagirica indicated in vitro DPPH scavenging (23.12 ± 1.28 µg/mL), ABTS scavenging (27.44 ± 1.88 µg/mL), H2O2 scavenging (12.92 ± 1.05 µg/mL), and FRAP (5.42 ± 0.19 µg/mL).
36296712	4	83	located	found	712:716	arg1	India					748:752	India	748:752	India	748:752	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	4	83	located	found	712:716	arg2	plant					691:695	a plant	689:695	a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus	689:791	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	4	83	located	found	712:716	arg2	nilagirica					675:684	Artemisia nilagirica	665:684	Artemisia nilagirica	665:684	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	4	84	theme	prominent	763:771	arg1	member					773:778	a prominent member	761:778	a prominent member of the genus	761:791	Artemisia nilagirica is a plant that is widely found in the Western Ghats, Kerala, India and is a prominent member of the genus.
36296712	2	85	theme	candidates	408:417	arg1	sources					392:398	sources	392:398	sources of drug candidates for modern medicine	392:437	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	85	theme	candidates	408:417	arg1	products					369:376	plant products	363:376	plant products	363:376	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	85	theme	candidates	408:417	arg1	plants					352:357	numerous plants	343:357	numerous plants	343:357	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	0	86	theme	Anti-Inflammatory	52:68	arg1	Activities					105:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities	52:114	Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke)	52:152	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	8	87	theme	aldose	1484:1489	arg1	reductase					1491:1499	aldose reductase	1484:1499	aldose reductase (17.42 ± 0.87 µg/mL)	1484:1520	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	8	87	theme	aldose	1484:1489	arg1	µg/mL					1515:1519	17.42 ± 0.87 µg/mL	1502:1519	17.42 ± 0.87 µg/mL	1502:1519	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	0	88	dep	In	30:31	arg1	Vitro					33:37	Vitro	33:37	Vitro	33:37	Phytochemical Composition and In Vitro Antioxidant, Anti-Inflammatory, Anticancer, and Enzyme-Inhibitory Activities of Artemisia nilagirica (C.B. Clarke) Pamp.
36296712	10	89	theme	polyphenols	1816:1826	arg1	level					1807:1811	a higher level	1798:1811	a higher level of polyphenols and flavonoids in the extract	1798:1856	The results of the phytochemical screening indicated a higher level of polyphenols and flavonoids in the extract and the LCMS analysis revealed the presence of various bioactive constituents including artemisinin.
36296712	8	90	theme	α-glucosidase	1448:1460	arg1	activities					1402:1411	the activities	1398:1411	the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL)	1398:1569	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	2	91	theme	numerous	343:350	arg1	plants					352:357	numerous plants	343:357	numerous plants	343:357	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	91	theme	numerous	343:350	arg1	products					369:376	plant products	363:376	plant products	363:376	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	91	theme	numerous	343:350	arg1	sources					392:398	sources	392:398	sources of drug candidates for modern medicine	392:437	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	92	used	utilized	334:341	arg2	systems					321:327	folk systems	316:327	folk systems	316:327	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	2	92	used	utilized	334:341	arg2	medicines					302:310	traditional medicines	290:310	traditional medicines	290:310	Various traditional medicines and folk systems have utilized numerous plants and plant products, which act as sources of drug candidates for modern medicine.
36296712	8	93	theme	±	1508:1508	arg1	reductase					1491:1499	aldose reductase	1484:1499	aldose reductase (17.42 ± 0.87 µg/mL)	1484:1520	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
36296712	8	93	theme	±	1508:1508	arg1	µg/mL					1515:1519	17.42 ± 0.87 µg/mL	1502:1519	17.42 ± 0.87 µg/mL	1502:1519	A. nilagirica was also efficient in inhibiting the activities of α-amylase (38.42 ± 2.71 µg/mL), α-glucosidase (55.31 ± 2.16 µg/mL), aldose reductase (17.42 ± 0.87 µg/mL), and sorbitol dehydrogenase (29.57 ± 1.46 µg/mL).
37278652	4	0	theme	low	771:773	arg1	pH					775:776	a mildly low pH	762:776	a mildly low pH	762:776	Moreover, lipid A of the bacteria grown at a mildly low pH is modified by adding 4-amino-arabinose (l-Ara4N).
37278652	11	1	dep	changes	2172:2178	arg1	undergoes					2194:2202	undergoes	2194:2202	undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment	2194:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	1	2	theme	environmental	248:260	arg1	stress					262:267	environmental stress	248:267	environmental stress	248:267	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	11	3	theme	moderate	1972:1979	arg1	decrease					1981:1988	a moderate decrease	1970:1988	a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment	1970:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	3	4	theme	virulence	667:675	arg1	arnT					649:652	arnT	649:652	arnT	649:652	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	4	theme	virulence	667:675	arg1	pagP					658:661	pagP	658:661	pagP	658:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	4	theme	virulence	667:675	arg1	genes					677:681	virulence genes	667:681	virulence genes	667:681	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	13	5	from	changes	2451:2457	arg1	bacterium					2466:2474	the bacterium	2462:2474	the bacterium	2462:2474	Thus, considering physiological changes in the bacterium at low pH helps design and implement antimicrobial approaches against this hostile microorganism.
37278652	7	6	from	studies	1131:1137	arg1	permeability					1171:1182	permeability	1171:1182	permeability	1171:1182	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	7	6	from	studies	1131:1137	arg1	viscosity					1157:1165	inner membrane viscosity	1142:1165	inner membrane viscosity	1142:1165	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	13	7	theme	hostile	2551:2557	arg1	microorganism					2559:2571	this hostile microorganism	2546:2571	this hostile microorganism	2546:2571	Thus, considering physiological changes in the bacterium at low pH helps design and implement antimicrobial approaches against this hostile microorganism.
37278652	6	8	theme	low	1040:1042	arg1	pH					1044:1045	a mildly low pH	1031:1045	a mildly low pH	1031:1045	Interestingly, at a mildly low pH, P. aeruginosa produces a thicker biofilm with higher biofilm biomass.
37278652	11	9	theme	membrane	2123:2130	arg1	composition					2072:2082	lipid A composition	2064:2082	lipid A composition	2064:2082	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	11	9	theme	membrane	2123:2130	arg1	reduction					2090:2098	a reduction	2088:2098	a reduction of the bacterial inner membrane	2088:2130	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	13	10	theme	antimicrobial	2513:2525	arg1	approaches					2527:2536	design and implement antimicrobial approaches	2492:2536	approaches	2527:2536	Thus, considering physiological changes in the bacterium at low pH helps design and implement antimicrobial approaches against this hostile microorganism.
37278652	3	11	dep	genes	583:587	arg1	arnT					649:652	arnT	649:652	arnT	649:652	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	11	dep	genes	583:587	arg1	genes					583:587	two-component system genes	562:587	two-component system genes (phoP/phoQ and pmrA/pmrB)	562:613	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	11	dep	genes	583:587	arg1	pmrA/pmrB					604:612	pmrA/pmrB	604:612	pmrA/pmrB	604:612	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	11	dep	genes	583:587	arg1	pagP					658:661	pagP	658:661	pagP	658:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	11	dep	genes	583:587	arg1	phoP/phoQ					590:598	phoP/phoQ	590:598	phoP/phoQ	590:598	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	7	12	theme	membrane	1243:1250	arg1	permeability					1252:1263	the inner membrane permeability	1233:1263	the inner membrane permeability	1233:1263	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	11	13	dep	pH.	2011:2013	arg1	changes					2172:2178	the changes	2168:2178	the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment	2168:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	10	14	theme	acidic	1865:1870	arg1	pH					1872:1873	acidic pH	1865:1873	acidic pH	1865:1873	IMPORTANCE P. aeruginosa encounters environments with acidic pH when establishing infections in hosts.
37278652	5	15	theme	low-pH	968:973	arg1	environment					975:985	a mildly low-pH environment	959:985	a mildly low-pH environment	959:985	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	3	16	theme	two-component	562:574	arg1	arnT					649:652	arnT	649:652	arnT	649:652	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	16	theme	two-component	562:574	arg1	genes					583:587	two-component system genes	562:587	two-component system genes (phoP/phoQ and pmrA/pmrB)	562:613	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	16	theme	two-component	562:574	arg1	pmrA/pmrB					604:612	pmrA/pmrB	604:612	pmrA/pmrB	604:612	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	16	theme	two-component	562:574	arg1	pagP					658:661	pagP	658:661	pagP	658:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	16	theme	two-component	562:574	arg1	phoP/phoQ					590:598	phoP/phoQ	590:598	phoP/phoQ	590:598	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	6	17	theme	higher	1094:1099	arg1	biomass					1109:1115	higher biofilm biomass	1094:1115	higher biofilm biomass	1094:1115	Interestingly, at a mildly low pH, P. aeruginosa produces a thicker biofilm with higher biofilm biomass.
37278652	10	18	with	environments	1847:1858	arg1	pH					1872:1873	acidic pH	1865:1873	acidic pH	1865:1873	IMPORTANCE P. aeruginosa encounters environments with acidic pH when establishing infections in hosts.
37278652	12	19	dep	P.	2341:2342	arg1	aeruginosa					2344:2353	aeruginosa	2344:2353	aeruginosa	2344:2353	Overall, these alterations in the P. aeruginosa phenotype put obstacles in the way of antibacterial activities.
37278652	3	20	theme	genes	583:587	arg1	expression					548:557	expression	548:557	expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA,	548:703	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	2	21	theme	neutral	468:474	arg1	medium					476:481	a neutral medium	466:481	a neutral medium (pH 7.2)	466:490	This study investigated the modifications that P. aeruginosa undertakes at a mildly low pH (pH 5.0) compared with the bacteria grown in a neutral medium (pH 7.2).
37278652	2	21	theme	neutral	468:474	arg1	pH					484:485	pH 7.2	484:489	pH 7.2	484:489	This study investigated the modifications that P. aeruginosa undertakes at a mildly low pH (pH 5.0) compared with the bacteria grown in a neutral medium (pH 7.2).
37278652	11	22	from	level	2022:2026	arg1	changes					2172:2178	the changes	2168:2178	the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment	2168:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	13	23	theme	design	2492:2497	arg1	approaches					2527:2536	design and implement antimicrobial approaches	2492:2536	approaches	2527:2536	Thus, considering physiological changes in the bacterium at low pH helps design and implement antimicrobial approaches against this hostile microorganism.
37278652	8	24	theme	PmrB	1351:1354	arg1	importance					1315:1324	the importance	1311:1324	the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress	1311:1412	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	11	25	dep	likely	2252:2257	arg1	form					2262:2265	form	2262:2265	to form biofilm in a mildly acidic environment	2259:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	5	26	theme	virulence	861:869	arg1	alginate					900:907	alginate	900:907	alginate	900:907	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	5	26	theme	virulence	861:869	arg1	factors					871:877	virulence factors	861:877	virulence factors such as rhamnolipid, alginate, and membrane vesicles	861:930	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	5	26	theme	virulence	861:869	arg1	rhamnolipid					887:897	rhamnolipid	887:897	rhamnolipid	887:897	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	5	26	theme	virulence	861:869	arg1	vesicles					923:930	membrane vesicles	914:930	membrane vesicles	914:930	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	11	27	theme	reduction	2090:2098	arg1	permeability					2132:2143	modified lipid A composition and a reduction of the bacterial inner membrane permeability	2055:2143	modified lipid A composition and a reduction of the bacterial inner membrane permeability	2055:2143	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	8	28	theme	Gram-negative	1359:1371	arg1	bacteria					1373:1380	Gram-negative bacteria	1359:1380	Gram-negative bacteria	1359:1380	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	0	29	theme	Surface	107:113	arg1	Remodeling					79:88	Remodeling	79:88	Remodeling of the Bacterial Surface	79:113	A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.
37278652	12	30	from	alterations	2322:2332	arg1	phenotype					2355:2363	the P. aeruginosa phenotype	2337:2363	the P. aeruginosa phenotype	2337:2363	Overall, these alterations in the P. aeruginosa phenotype put obstacles in the way of antibacterial activities.
37278652	7	31	from	decrease	1221:1228	arg1	permeability					1252:1263	the inner membrane permeability	1233:1263	the inner membrane permeability	1233:1263	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	12	32	theme	antibacterial	2393:2405	arg1	activities					2407:2416	antibacterial activities	2393:2416	antibacterial activities	2393:2416	Overall, these alterations in the P. aeruginosa phenotype put obstacles in the way of antibacterial activities.
37278652	8	33	from	PmrB	1351:1354	arg1	bacteria					1373:1380	Gram-negative bacteria	1359:1380	Gram-negative bacteria	1359:1380	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	8	34	dep	P.	1534:1535	arg1	aeruginosa					1537:1546	aeruginosa	1537:1546	aeruginosa	1537:1546	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	0	35	theme	Acidic	9:14	arg1	Environment					16:26	A Mildly Acidic Environment	0:26	A Mildly Acidic Environment	0:26	A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.
37278652	11	36	theme	modified	2055:2062	arg1	permeability					2132:2143	modified lipid A composition and a reduction of the bacterial inner membrane permeability	2055:2143	modified lipid A composition and a reduction of the bacterial inner membrane permeability	2055:2143	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	8	37	theme	pH	1404:1405	arg1	stress					1407:1412	low pH stress	1400:1412	low pH stress	1400:1412	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	1	38	theme	altered	293:299	arg1	phenotype					319:327	an altered virulence-related phenotype	290:327	an altered virulence-related phenotype	290:327	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	11	39	theme	envelope	2045:2052	arg1	level					2022:2026	the level	2018:2026	the level of the bacterial envelope	2018:2052	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	3	40	theme	genes	635:639	arg1	expression					548:557	expression	548:557	expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA,	548:703	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	4	41	theme	lipid	729:733	arg1	A					735:735	lipid A	729:735	lipid A of the bacteria grown at a mildly low pH	729:776	Moreover, lipid A of the bacteria grown at a mildly low pH is modified by adding 4-amino-arabinose (l-Ara4N).
37278652	7	42	theme	inner	1142:1146	arg1	viscosity					1157:1165	inner membrane viscosity	1142:1165	inner membrane viscosity	1142:1165	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	12	43	theme	P.	2341:2342	arg1	phenotype					2355:2363	the P. aeruginosa phenotype	2337:2363	the P. aeruginosa phenotype	2337:2363	Overall, these alterations in the P. aeruginosa phenotype put obstacles in the way of antibacterial activities.
37278652	3	44	theme	A	622:622	arg1	arnT					649:652	arnT	649:652	arnT	649:652	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	44	theme	A	622:622	arg1	pagP					658:661	pagP	658:661	pagP	658:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	44	theme	A	622:622	arg1	genes					635:639	lipid A remodeling genes	616:639	lipid A remodeling genes such as arnT and pagP	616:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	9	45	theme	antibacterial	1763:1775	arg1	strategies					1777:1786	antibacterial strategies	1763:1786	antibacterial strategies against P. aeruginosa	1763:1808	Given that P. aeruginosa is likely to encounter mildly acidic environments during infection in its host, the alterations that the bacterium undertakes under such conditions must be considered in designing antibacterial strategies against P. aeruginosa.
37278652	9	46	theme	such	1715:1718	arg1	conditions					1720:1729	such conditions	1715:1729	such conditions	1715:1729	Given that P. aeruginosa is likely to encounter mildly acidic environments during infection in its host, the alterations that the bacterium undertakes under such conditions must be considered in designing antibacterial strategies against P. aeruginosa.
37278652	11	47	theme	A	2070:2070	arg1	composition					2072:2082	lipid A composition	2064:2082	lipid A composition	2064:2082	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	0	48	dep	Pseudomonas	35:45	arg1	aeruginosa					47:56	aeruginosa	47:56	aeruginosa	47:56	A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.
37278652	8	49	theme	two-component	1461:1473	arg1	systems					1475:1481	these two-component systems	1455:1481	these two-component systems	1455:1481	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	3	50	theme	genes	677:681	arg1	expression					548:557	expression	548:557	expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA,	548:703	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	8	51	theme	P.	1534:1535	arg1	envelope					1548:1555	the P. aeruginosa envelope	1530:1555	the P. aeruginosa envelope	1530:1555	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	1	52	theme	environmental	176:188	arg1	pH.					217:219	suboptimal pH.	206:219	suboptimal pH.	206:219	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	1	52	theme	environmental	176:188	arg1	stress					190:195	environmental stress	176:195	environmental stress	176:195	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	1	52	theme	environmental	176:188	arg1	result					226:231	a result	224:231	a result of exposure to environmental stress	224:267	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	9	53	from	infection	1640:1648	arg1	host					1657:1660	its host	1653:1660	its host	1653:1660	Given that P. aeruginosa is likely to encounter mildly acidic environments during infection in its host, the alterations that the bacterium undertakes under such conditions must be considered in designing antibacterial strategies against P. aeruginosa.
37278652	4	54	theme	bacteria	744:751	arg1	A					735:735	lipid A	729:735	lipid A of the bacteria grown at a mildly low pH	729:776	Moreover, lipid A of the bacteria grown at a mildly low pH is modified by adding 4-amino-arabinose (l-Ara4N).
37278652	11	55	theme	inner	2117:2121	arg1	membrane					2123:2130	the bacterial inner membrane	2103:2130	the bacterial inner membrane	2103:2130	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	3	56	theme	acidic	528:533	arg1	environment					535:545	a mildly acidic environment	519:545	a mildly acidic environment	519:545	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	8	57	theme	envelope	1548:1555	arg1	remodeling					1516:1525	the remodeling	1512:1525	the remodeling of the P. aeruginosa envelope	1512:1555	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	13	58	theme	implement	2503:2511	arg1	approaches					2527:2536	design and implement antimicrobial approaches	2492:2536	approaches	2527:2536	Thus, considering physiological changes in the bacterium at low pH helps design and implement antimicrobial approaches against this hostile microorganism.
37278652	11	59	theme	altered	1940:1946	arg1	phenotype					1948:1956	an altered phenotype	1937:1956	an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment	1937:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	8	60	theme	PhoP	1329:1332	arg1	importance					1315:1324	the importance	1311:1324	the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress	1311:1412	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	6	61	theme	biofilm	1101:1107	arg1	biomass					1109:1115	higher biofilm biomass	1094:1115	higher biofilm biomass	1094:1115	Interestingly, at a mildly low pH, P. aeruginosa produces a thicker biofilm with higher biofilm biomass.
37278652	11	62	from	decrease	1981:1988	arg1	pH.					2011:2013	pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment	2011:2304	the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment	1993:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	8	63	theme	PhoQ	1335:1338	arg1	importance					1315:1324	the importance	1311:1324	the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress	1311:1412	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	7	64	theme	inner	1237:1241	arg1	permeability					1252:1263	the inner membrane permeability	1233:1263	the inner membrane permeability	1233:1263	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	8	65	theme	PmrA	1341:1344	arg1	importance					1315:1324	the importance	1311:1324	the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress	1311:1412	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	6	66	theme	thicker	1073:1079	arg1	biofilm					1081:1087	a thicker biofilm	1071:1087	a thicker biofilm with higher biofilm biomass	1071:1115	Interestingly, at a mildly low pH, P. aeruginosa produces a thicker biofilm with higher biofilm biomass.
37278652	11	67	theme	acidic	2287:2292	arg1	environment					2294:2304	a mildly acidic environment	2278:2304	a mildly acidic environment	2278:2304	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	8	68	from	PhoP	1329:1332	arg1	bacteria					1373:1380	Gram-negative bacteria	1359:1380	Gram-negative bacteria	1359:1380	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	3	69	theme	system	576:581	arg1	arnT					649:652	arnT	649:652	arnT	649:652	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	69	theme	system	576:581	arg1	genes					583:587	two-component system genes	562:587	two-component system genes (phoP/phoQ and pmrA/pmrB)	562:613	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	69	theme	system	576:581	arg1	pmrA/pmrB					604:612	pmrA/pmrB	604:612	pmrA/pmrB	604:612	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	69	theme	system	576:581	arg1	pagP					658:661	pagP	658:661	pagP	658:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	69	theme	system	576:581	arg1	phoP/phoQ					590:598	phoP/phoQ	590:598	phoP/phoQ	590:598	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	1	70	theme	versatile	144:152	arg1	pathogen					154:161	a versatile pathogen	142:161	a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress	142:267	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	1	70	theme	versatile	144:152	arg1	aeruginosa					128:137	Pseudomonas aeruginosa	116:137	Pseudomonas aeruginosa	116:137	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	11	71	theme	bacterial	2107:2115	arg1	membrane					2123:2130	the bacterial inner membrane	2103:2130	the bacterial inner membrane	2103:2130	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	8	72	from	importance	1315:1324	arg1	bacteria					1373:1380	Gram-negative bacteria	1359:1380	Gram-negative bacteria	1359:1380	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	11	73	theme	composition	2072:2082	arg1	permeability					2132:2143	modified lipid A composition and a reduction of the bacterial inner membrane permeability	2055:2143	modified lipid A composition and a reduction of the bacterial inner membrane permeability	2055:2143	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	0	74	theme	Bacterial	97:105	arg1	Surface					107:113	the Bacterial Surface	93:113	the Bacterial Surface	93:113	A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.
37278652	3	75	dep	pqsE	690:693	arg1	i.e.					684:687	i.e.	684:687	i.e.	684:687	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	6	76	with	biofilm	1081:1087	arg1	biomass					1109:1115	higher biofilm biomass	1094:1115	higher biofilm biomass	1094:1115	Interestingly, at a mildly low pH, P. aeruginosa produces a thicker biofilm with higher biofilm biomass.
37278652	9	77	theme	acidic	1613:1618	arg1	environments					1620:1631	mildly acidic environments	1606:1631	mildly acidic environments	1606:1631	Given that P. aeruginosa is likely to encounter mildly acidic environments during infection in its host, the alterations that the bacterium undertakes under such conditions must be considered in designing antibacterial strategies against P. aeruginosa.
37278652	10	78	dep	IMPORTANCE	1811:1820	arg1	aeruginosa					1825:1834	P. aeruginosa	1822:1834	IMPORTANCE P. aeruginosa	1811:1834	IMPORTANCE P. aeruginosa encounters environments with acidic pH when establishing infections in hosts.
37278652	5	79	theme	factors	871:877	arg1	higher					949:954	higher	949:954	higher	949:954	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	5	79	theme	factors	871:877	arg1	production					847:856	the production	843:856	the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles	843:930	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	7	80	theme	low	1205:1207	arg1	pH					1209:1210	a mildly low pH	1196:1210	a mildly low pH	1196:1210	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	12	81	theme	activities	2407:2416	arg1	way					2386:2388	the way	2382:2388	the way of antibacterial activities	2382:2416	Overall, these alterations in the P. aeruginosa phenotype put obstacles in the way of antibacterial activities.
37278652	5	82	theme	neutral	997:1003	arg1	medium					1005:1010	a neutral medium	995:1010	a neutral medium	995:1010	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	11	83	theme	bacterial	2035:2043	arg1	envelope					2045:2052	the bacterial envelope	2031:2052	the bacterial envelope	2031:2052	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	8	84	theme	low	1400:1402	arg1	stress					1407:1412	low pH stress	1400:1412	low pH stress	1400:1412	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	13	85	theme	physiological	2437:2449	arg1	changes					2451:2457	physiological changes	2437:2457	physiological changes in the bacterium	2437:2474	Thus, considering physiological changes in the bacterium at low pH helps design and implement antimicrobial approaches against this hostile microorganism.
37278652	0	86	theme	Pseudomonas	35:45	arg1	Virulence					58:66	Pseudomonas aeruginosa Virulence	35:66	Pseudomonas aeruginosa Virulence	35:66	A Mildly Acidic Environment Alters Pseudomonas aeruginosa Virulence and Causes Remodeling of the Bacterial Surface.
37278652	11	87	theme	lipid	2064:2068	arg1	composition					2072:2082	lipid A composition	2064:2082	lipid A composition	2064:2082	The bacterium develops an altered phenotype to tolerate a moderate decrease in the environmental pH. At the level of the bacterial envelope, modified lipid A composition and a reduction of the bacterial inner membrane permeability and fluidity are among the changes P. aeruginosa undergoes at a mildly low pH. Also, the bacterium is more likely to form biofilm in a mildly acidic environment.
37278652	1	88	dep	such	198:201	arg1	as					203:204	as	203:204	as	203:204	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	2	89	theme	low	414:416	arg1	pH					418:419	a mildly low pH	405:419	a mildly low pH (pH 5.0)	405:428	This study investigated the modifications that P. aeruginosa undertakes at a mildly low pH (pH 5.0) compared with the bacteria grown in a neutral medium (pH 7.2).
37278652	2	89	theme	low	414:416	arg1	pH					422:423	pH 5.0	422:427	pH 5.0	422:427	This study investigated the modifications that P. aeruginosa undertakes at a mildly low pH (pH 5.0) compared with the bacteria grown in a neutral medium (pH 7.2).
37278652	8	90	from	bacteria	1373:1380	arg1	importance					1315:1324	the importance	1311:1324	the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress	1311:1412	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	1	91	theme	virulence-related	301:317	arg1	phenotype					319:327	an altered virulence-related phenotype	290:327	an altered virulence-related phenotype	290:327	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	8	92	from	PmrA	1341:1344	arg1	bacteria					1373:1380	Gram-negative bacteria	1359:1380	Gram-negative bacteria	1359:1380	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	5	93	theme	membrane	914:921	arg1	vesicles					923:930	membrane vesicles	914:930	membrane vesicles	914:930	Additionally, the production of virulence factors such as rhamnolipid, alginate, and membrane vesicles is significantly higher in a mildly low-pH environment than in a neutral medium.
37278652	7	94	theme	membrane	1148:1155	arg1	viscosity					1157:1165	inner membrane viscosity	1142:1165	inner membrane viscosity	1142:1165	Furthermore, studies on inner membrane viscosity and permeability showed that a mildly low pH causes a decrease in the inner membrane permeability and increases its viscosity.
37278652	13	95	theme	low	2479:2481	arg1	pH					2483:2484	low pH	2479:2484	low pH	2479:2484	Thus, considering physiological changes in the bacterium at low pH helps design and implement antimicrobial approaches against this hostile microorganism.
37278652	3	96	theme	remodeling	624:633	arg1	arnT					649:652	arnT	649:652	arnT	649:652	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	96	theme	remodeling	624:633	arg1	pagP					658:661	pagP	658:661	pagP	658:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	96	theme	remodeling	624:633	arg1	genes					635:639	lipid A remodeling genes	616:639	lipid A remodeling genes such as arnT and pagP	616:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	97	theme	lipid	616:620	arg1	arnT					649:652	arnT	649:652	arnT	649:652	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	97	theme	lipid	616:620	arg1	pagP					658:661	pagP	658:661	pagP	658:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	3	97	theme	lipid	616:620	arg1	genes					635:639	lipid A remodeling genes	616:639	lipid A remodeling genes such as arnT and pagP	616:661	Results indicated that in a mildly acidic environment, expression of two-component system genes (phoP/phoQ and pmrA/pmrB), lipid A remodeling genes such as arnT and pagP and virulence genes, i.e., pqsE and rhlA, were induced.
37278652	8	98	theme	each	1447:1450	arg1	absence					1436:1442	the absence	1432:1442	the absence of each of these two-component systems	1432:1481	Besides, despite the importance of PhoP, PhoQ, PmrA, and PmrB in Gram-negative bacteria for responding to low pH stress, we observed that the absence of each of these two-component systems does not meaningfully impact the remodeling of the P. aeruginosa envelope.
37278652	1	99	theme	exposure	236:243	arg1	pH.					217:219	suboptimal pH.	206:219	suboptimal pH.	206:219	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	1	99	theme	exposure	236:243	arg1	result					226:231	a result	224:231	a result of exposure to environmental stress	224:267	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
37278652	1	99	theme	exposure	236:243	arg1	stress					190:195	environmental stress	176:195	environmental stress	176:195	Pseudomonas aeruginosa is a versatile pathogen that resists environmental stress, such as suboptimal pH. As a result of exposure to environmental stress, P. aeruginosa shows an altered virulence-related phenotype.
36706451	4	0	theme	biomaterial	890:900	arg1	ink					902:904	a composite biomaterial ink	878:904	a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications	878:1028	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	10	1	theme	Particle-matrix	1574:1588	arg1	integration					1590:1600	Particle-matrix integration	1574:1600	Particle-matrix integration	1574:1600	Particle-matrix integration was confirmed via scanning electron microscopy.
36706451	2	2	theme	scale	383:387	arg1	gelatin					389:395	fish scale gelatin	378:395	fish scale gelatin	378:395	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	12	3	theme	engineering	2088:2098	arg1	applications					2100:2111	bone engineering applications	2083:2111	bone engineering applications	2083:2111	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	6	4	theme	inks	1208:1211	arg1	properties					1158:1167	the physicochemical and mechanical properties	1123:1167	the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1123:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	10	5	theme	electron	1629:1636	arg1	microscopy					1638:1647	scanning electron microscopy	1620:1647	scanning electron microscopy	1620:1647	Particle-matrix integration was confirmed via scanning electron microscopy.
36706451	2	6	theme	proteinous	325:334	arg1	gelatin					389:395	fish scale gelatin	378:395	fish scale gelatin	378:395	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	6	theme	proteinous	325:334	arg1	collagen					365:372	jellyfish collagen	355:372	jellyfish collagen	355:372	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	6	theme	proteinous	325:334	arg1	materials					336:344	animal-derived proteinous materials	310:344	animal-derived proteinous materials	310:344	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	6	7	theme	ADA-GEL	1188:1194	arg1	inks					1208:1211	FS incorporated ADA-GEL biomaterial inks	1172:1211	FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1172:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	11	8	theme	MC3T3-E1	1733:1740	arg1	cells					1742:1746	MC3T3-E1 cells	1733:1746	MC3T3-E1 cells	1733:1746	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	12	9	theme	ink	2064:2066	arg1	properties					2068:2077	the biomaterial ink properties	2048:2077	the biomaterial ink properties for bone engineering applications	2048:2111	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	6	10	theme	FS	1172:1173	arg1	inks					1208:1211	FS incorporated ADA-GEL biomaterial inks	1172:1211	FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1172:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	2	11	link	animal-derived	310:323	arg1	gelatin					389:395	fish scale gelatin	378:395	fish scale gelatin	378:395	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	11	link	animal-derived	310:323	arg1	collagen					365:372	jellyfish collagen	355:372	jellyfish collagen	355:372	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	11	link	animal-derived	310:323	arg1	materials					336:344	animal-derived proteinous materials	310:344	animal-derived proteinous materials	310:344	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	6	12	theme	cell-laden	1265:1274	arg1	hydrogels					1276:1284	cell-laden hydrogels	1265:1284	cell-laden hydrogels	1265:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	1	13	theme	matrix	216:221	arg1	properties					184:193	the physicochemical properties	164:193	the physicochemical properties of the extracellular matrix	164:221	The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications.
36706451	7	14	theme	particle	1427:1434	arg1	content					1436:1442	FS particle content	1424:1442	FS particle content	1424:1442	Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content.
36706451	11	15	theme	MC3T3-E1	1862:1869	arg1	cells					1871:1875	MC3T3-E1 cells	1862:1875	MC3T3-E1 cells	1862:1875	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	13	16	theme	bioprinting	2225:2235	arg1	applications					2237:2248	3D bioprinting applications	2222:2248	3D bioprinting applications	2222:2248	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	3	17	theme	fish	586:589	arg1	scales					591:596	structurally intact fish scales	566:596	structurally intact fish scales	566:596	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	4	18	theme	alginate	938:945	arg1	ADA					959:961	ADA	959:961	ADA	959:961	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	4	18	theme	alginate	938:945	arg1	dialdehyde					947:956	alginate dialdehyde	938:956	alginate dialdehyde (ADA)-gelatin (GEL)	938:976	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	5	19	theme	mouse	1074:1078	arg1	cells					1104:1108	mouse pre-osteoblast MC3T3-E1 cells	1074:1108	mouse pre-osteoblast MC3T3-E1 cells	1074:1108	We fabricate 3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells.
36706451	9	20	theme	FS	1509:1510	arg1	particles					1512:1520	FS particles	1509:1520	FS particles	1509:1520	FS particles were homogeneously incorporated into the hydrogels.
36706451	4	21	theme	fish	918:921	arg1	scale					923:927	fish scale	918:927	fish scale (FS)	918:932	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	4	21	theme	fish	918:921	arg1	FS					930:931	FS	930:931	FS	930:931	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	3	22	theme	mechanical	788:797	arg1	strength					799:806	mechanical strength	788:806	mechanical strength in 3D printing applications	788:834	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	13	23	theme	great	2202:2206	arg1	potential					2208:2216	great potential	2202:2216	great potential for 3D bioprinting applications	2202:2248	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	8	24	theme	hydrogel	1489:1496	arg1	stiffness					1498:1506	hydrogel stiffness	1489:1506	hydrogel stiffness	1489:1506	Addition of FS also provides a tool to tune hydrogel stiffness.
36706451	7	25	theme	hydrogels	1374:1382	arg1	strength					1358:1365	the compressive strength	1342:1365	the compressive strength of the hydrogels	1342:1382	Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content.
36706451	11	26	theme	ALP	1820:1822	arg1	activity					1824:1831	increased ALP activity	1810:1831	increased ALP activity	1810:1831	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	5	27	theme	3D	1044:1045	arg1	hydrogels					1058:1066	3D cell-laden hydrogels	1044:1066	3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells	1044:1108	We fabricate 3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells.
36706451	3	28	theme	printing	814:821	arg1	applications					823:834	3D printing applications	811:834	3D printing applications	811:834	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	0	29	theme	tissue	66:71	arg1	engineering					73:83	bone tissue engineering	61:83	bone tissue engineering	61:83	Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering.
36706451	13	30	theme	biomaterial	2169:2179	arg1	formulation					2185:2195	a new biomaterial ink formulation	2163:2195	a new biomaterial ink formulation with great potential for 3D bioprinting applications	2163:2248	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	13	31	with	formulation	2185:2195	arg1	potential					2208:2216	great potential	2202:2216	great potential for 3D bioprinting applications	2202:2248	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	12	32	theme	bone	2083:2086	arg1	applications					2100:2111	bone engineering applications	2083:2111	bone engineering applications	2083:2111	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	12	33	theme	natural	1990:1996	arg1	collagen					1998:2005	a natural collagen and hydroxyapatite source	1988:2031	collagen	1998:2005	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	5	34	theme	MC3T3-E1	1095:1102	arg1	cells					1104:1108	mouse pre-osteoblast MC3T3-E1 cells	1074:1108	mouse pre-osteoblast MC3T3-E1 cells	1074:1108	We fabricate 3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells.
36706451	12	35	theme	FS	1972:1973	arg1	structure					1975:1983	FS structure	1972:1983	FS structure as a natural collagen and hydroxyapatite source	1972:2031	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	4	36	theme	bioprinting	1005:1015	arg1	applications					1017:1028	three-dimensional (3D) bioprinting applications	982:1028	three-dimensional (3D) bioprinting applications	982:1028	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	13	37	theme	3D	2222:2223	arg1	applications					2237:2248	3D bioprinting applications	2222:2248	3D bioprinting applications	2222:2248	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	0	38	theme	containing	11:20	arg1	bioink					50:55	containing alginate dialdehyde-gelatin bioink	11:55	containing alginate dialdehyde-gelatin bioink for bone tissue engineering	11:83	Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering.
36706451	7	39	theme	FS	1338:1339	arg1	orientation					1319:1329	the distinctive collagen orientation	1294:1329	the distinctive collagen orientation of the FS	1294:1339	Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content.
36706451	4	40	theme	three-dimensional	982:998	arg1	applications					1017:1028	three-dimensional (3D) bioprinting applications	982:1028	three-dimensional (3D) bioprinting applications	982:1028	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	2	41	theme	biomaterial	432:442	arg1	design					448:453	biomaterial ink design	432:453	biomaterial ink design	432:453	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	0	42	theme	dialdehyde-gelatin	31:48	arg1	bioink					50:55	containing alginate dialdehyde-gelatin bioink	11:55	containing alginate dialdehyde-gelatin bioink for bone tissue engineering	11:83	Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering.
36706451	13	43	theme	tissue	2302:2307	arg1	engineering					2309:2319	bone tissue engineering	2297:2319	bone tissue engineering	2297:2319	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	12	44	theme	hydrogels	1961:1969	arg1	stiffness					1913:1921	stiffness	1913:1921	stiffness	1913:1921	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	12	44	theme	hydrogels	1961:1969	arg1	osteoinductivity					1937:1952	supported osteoinductivity	1927:1952	supported osteoinductivity	1927:1952	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	2	45	theme	hydrogels	486:494	arg1	bioactivity					471:481	the bioactivity	467:481	the bioactivity of hydrogels	467:494	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	11	46	theme	pristine	1765:1772	arg1	ADA-GEL					1774:1780	pristine ADA-GEL	1765:1780	pristine ADA-GEL	1765:1780	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	2	47	theme	fish	378:381	arg1	gelatin					389:395	fish scale gelatin	378:395	fish scale gelatin	378:395	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	48	from	pillar	422:427	arg1	design					448:453	biomaterial ink design	432:453	biomaterial ink design	432:453	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	6	49	theme	physicochemical	1127:1141	arg1	properties					1158:1167	the physicochemical and mechanical properties	1123:1167	the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1123:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	8	50	theme	FS	1457:1458	arg1	Addition					1445:1452	Addition	1445:1452	Addition of FS	1445:1458	Addition of FS also provides a tool to tune hydrogel stiffness.
36706451	11	51	theme	FS	1650:1651	arg1	incorporation					1653:1665	FS incorporation	1650:1665	FS incorporation in the ADA-GEL matrix	1650:1687	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	13	52	theme	promising	2274:2282	arg1	applications					2321:2332	promising additive for bone tissue engineering applications	2274:2332	promising additive for bone tissue engineering applications	2274:2332	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	13	52	theme	promising	2274:2282	arg1	FS					2255:2256	FS	2255:2256	FS	2255:2256	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	12	53	theme	supported	1927:1935	arg1	osteoinductivity					1937:1952	supported osteoinductivity	1927:1952	supported osteoinductivity	1927:1952	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	1	54	dep	inks	117:120	arg1	essential					226:234	essential	226:234	essential	226:234	The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications.
36706451	11	55	theme	FS	1786:1787	arg1	incorporation					1789:1801	FS incorporation	1786:1801	FS incorporation	1786:1801	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	3	56	theme	structures	543:552	arg1	extraction					518:527	the extraction	514:527	the extraction of proteinous structures	514:552	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	1	57	theme	tissue	266:271	arg1	applications					285:296	tissue engineering applications	266:296	tissue engineering applications	266:296	The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications.
36706451	7	58	theme	collagen	1310:1317	arg1	orientation					1319:1329	the distinctive collagen orientation	1294:1329	the distinctive collagen orientation of the FS	1294:1339	Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content.
36706451	3	59	theme	inorganic	720:728	arg1	contents					747:754	its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents	681:754	its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents	681:754	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	11	60	theme	ADA-GEL	1674:1680	arg1	matrix					1682:1687	the ADA-GEL matrix	1670:1687	the ADA-GEL matrix	1670:1687	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	3	61	dep	organic	685:691	arg1	hydroxyapatite					731:744	hydroxyapatite	731:744	hydroxyapatite	731:744	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	3	61	dep	organic	685:691	arg1	gelatin					707:713	gelatin	707:713	gelatin	707:713	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	3	61	dep	organic	685:691	arg1	collagen					694:701	collagen	694:701	collagen	694:701	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	4	62	theme	composite	880:888	arg1	ink					902:904	a composite biomaterial ink	878:904	a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications	878:1028	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	11	63	theme	osteogenic	1703:1712	arg1	differentiation					1714:1728	the osteogenic differentiation	1699:1728	the osteogenic differentiation of MC3T3-E1 cells	1699:1746	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	4	64	dep	three-dimensional	982:998	arg1	3D					1001:1002	3D	1001:1002	3D	1001:1002	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	2	65	theme	materials	336:344	arg1	use					303:305	The use	299:305	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin,	299:396	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	66	theme	jellyfish	355:363	arg1	collagen					365:372	jellyfish collagen	355:372	jellyfish collagen	355:372	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	6	67	theme	biomaterial	1196:1206	arg1	inks					1208:1211	FS incorporated ADA-GEL biomaterial inks	1172:1211	FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1172:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	10	68	theme	scanning	1620:1627	arg1	microscopy					1638:1647	scanning electron microscopy	1620:1647	scanning electron microscopy	1620:1647	Particle-matrix integration was confirmed via scanning electron microscopy.
36706451	2	69	theme	animal-derived	310:323	arg1	gelatin					389:395	fish scale gelatin	378:395	fish scale gelatin	378:395	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	69	theme	animal-derived	310:323	arg1	collagen					365:372	jellyfish collagen	355:372	jellyfish collagen	355:372	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	2	69	theme	animal-derived	310:323	arg1	materials					336:344	animal-derived proteinous materials	310:344	animal-derived proteinous materials	310:344	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	6	70	theme	incorporated	1175:1186	arg1	inks					1208:1211	FS incorporated ADA-GEL biomaterial inks	1172:1211	FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1172:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	11	71	theme	cells	1742:1746	arg1	differentiation					1714:1728	the osteogenic differentiation	1699:1728	the osteogenic differentiation of MC3T3-E1 cells	1699:1746	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	6	72	theme	hydrogels	1276:1284	arg1	cytocompatibility					1244:1260	cytocompatibility	1244:1260	cytocompatibility	1244:1260	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	6	72	theme	hydrogels	1276:1284	arg1	bioactivity					1228:1238	bioactivity	1228:1238	bioactivity	1228:1238	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	6	72	theme	hydrogels	1276:1284	arg1	inks					1208:1211	FS incorporated ADA-GEL biomaterial inks	1172:1211	FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1172:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	3	73	theme	scales	591:596	arg1	use					559:561	the use	555:561	the use of structurally intact fish scales as an additive	555:611	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	1	74	theme	extracellular	202:214	arg1	matrix					216:221	the extracellular matrix	198:221	the extracellular matrix	198:221	The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications.
36706451	12	75	theme	biomaterial	2052:2062	arg1	properties					2068:2077	the biomaterial ink properties	2048:2077	the biomaterial ink properties for bone engineering applications	2048:2111	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	3	76	theme	intact	579:584	arg1	scales					591:596	structurally intact fish scales	566:596	structurally intact fish scales	566:596	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	12	77	theme	increased	1903:1911	arg1	stiffness					1913:1921	stiffness	1913:1921	stiffness	1913:1921	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	6	78	theme	mechanical	1147:1156	arg1	properties					1158:1167	the physicochemical and mechanical properties	1123:1167	the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1123:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	11	79	theme	cells	1871:1875	arg1	activity					1824:1831	increased ALP activity	1810:1831	increased ALP activity	1810:1831	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	11	79	theme	cells	1871:1875	arg1	secretion					1849:1857	osteocalcin secretion	1837:1857	osteocalcin secretion	1837:1857	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	11	80	from	incorporation	1653:1665	arg1	matrix					1682:1687	the ADA-GEL matrix	1670:1687	the ADA-GEL matrix	1670:1687	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	7	81	theme	FS	1424:1425	arg1	content					1436:1442	FS particle content	1424:1442	FS particle content	1424:1442	Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content.
36706451	3	82	theme	3D	811:812	arg1	applications					823:834	3D printing applications	811:834	3D printing applications	811:834	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	5	83	theme	pre-osteoblast	1080:1093	arg1	cells					1104:1108	mouse pre-osteoblast MC3T3-E1 cells	1074:1108	mouse pre-osteoblast MC3T3-E1 cells	1074:1108	We fabricate 3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells.
36706451	6	84	theme	bioactivity	1228:1238	arg1	properties					1158:1167	the physicochemical and mechanical properties	1123:1167	the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels	1123:1284	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	5	85	theme	cell-laden	1047:1056	arg1	hydrogels					1058:1066	3D cell-laden hydrogels	1044:1066	3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells	1044:1108	We fabricate 3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells.
36706451	6	86	dep	bioactivity	1228:1238	arg1	the					1224:1226	the	1224:1226	the	1224:1226	We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels.
36706451	11	87	theme	increased	1810:1818	arg1	activity					1824:1831	increased ALP activity	1810:1831	increased ALP activity	1810:1831	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	13	88	theme	ink	2181:2183	arg1	formulation					2185:2195	a new biomaterial ink formulation	2163:2195	a new biomaterial ink formulation with great potential for 3D bioprinting applications	2163:2248	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	1	89	theme	physicochemical	168:182	arg1	properties					184:193	the physicochemical properties	164:193	the physicochemical properties of the extracellular matrix	164:221	The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications.
36706451	3	90	from	strength	799:806	arg1	applications					823:834	3D printing applications	811:834	3D printing applications	811:834	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	12	91	theme	hydroxyapatite	2011:2024	arg1	source					2026:2031	a natural collagen and hydroxyapatite source	1988:2031	source	2026:2031	Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications.
36706451	4	92	theme	dialdehyde	947:956	arg1	GEL					973:975	GEL	973:975	GEL	973:975	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	4	92	theme	dialdehyde	947:956	arg1	-gelatin					963:970	alginate dialdehyde (ADA)-gelatin	938:970	alginate dialdehyde (ADA)-gelatin (GEL)	938:976	To test this hypothesis, we present here a composite biomaterial ink composed of fish scale (FS) and alginate dialdehyde (ADA)-gelatin (GEL) for three-dimensional (3D) bioprinting applications.
36706451	11	93	theme	osteocalcin	1837:1847	arg1	secretion					1849:1857	osteocalcin secretion	1837:1857	osteocalcin secretion	1837:1857	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	7	94	theme	compressive	1346:1356	arg1	strength					1358:1365	the compressive strength	1342:1365	the compressive strength of the hydrogels	1342:1382	Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content.
36706451	2	95	theme	important	412:420	arg1	pillar					422:427	an important pillar	409:427	an important pillar in biomaterial ink design to increase the bioactivity of hydrogels	409:494	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	13	96	theme	new	2165:2167	arg1	formulation					2185:2195	a new biomaterial ink formulation	2163:2195	a new biomaterial ink formulation with great potential for 3D bioprinting applications	2163:2248	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	0	97	theme	alginate	22:29	arg1	bioink					50:55	containing alginate dialdehyde-gelatin bioink	11:55	containing alginate dialdehyde-gelatin bioink for bone tissue engineering	11:83	Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering.
36706451	1	98	theme	biomaterial	105:115	arg1	development					90:100	The development	86:100	The development of biomaterial	86:115	The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications.
36706451	2	99	theme	ink	444:446	arg1	design					448:453	biomaterial ink design	432:453	biomaterial ink design	432:453	The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale gelatin, has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels.
36706451	3	100	theme	hydrogels	664:672	arg1	bioactivity					649:659	bioactivity	649:659	bioactivity	649:659	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	3	100	theme	hydrogels	664:672	arg1	biocompatibility					628:643	biocompatibility	628:643	biocompatibility	628:643	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	11	101	dep	ADA-GEL	1774:1780	arg1	comparison					1751:1760	comparison	1751:1760	comparison	1751:1760	FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells.
36706451	0	102	theme	bone	61:64	arg1	engineering					73:83	bone tissue engineering	61:83	bone tissue engineering	61:83	Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering.
36706451	7	103	theme	distinctive	1298:1308	arg1	orientation					1319:1329	the distinctive collagen orientation	1294:1329	the distinctive collagen orientation of the FS	1294:1339	Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increases with increasing FS particle content.
36706451	3	104	theme	proteinous	532:541	arg1	structures					543:552	proteinous structures	532:552	proteinous structures	532:552	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	3	105	theme	organic	685:691	arg1	contents					747:754	its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents	681:754	its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents	681:754	However, besides the extraction of proteinous structures, the use of structurally intact fish scales as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen and gelatin) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in 3D printing applications.
36706451	13	106	theme	additive	2284:2291	arg1	applications					2321:2332	promising additive for bone tissue engineering applications	2274:2332	promising additive for bone tissue engineering applications	2274:2332	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	13	106	theme	additive	2284:2291	arg1	FS					2255:2256	FS	2255:2256	FS	2255:2256	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	13	107	theme	bone	2297:2300	arg1	engineering					2309:2319	bone tissue engineering	2297:2319	bone tissue engineering	2297:2319	Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting applications, and FS is confirmed as promising additive for bone tissue engineering applications.
36706451	1	108	theme	engineering	273:283	arg1	applications					285:296	tissue engineering applications	266:296	tissue engineering applications	266:296	The development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for bioprinting technology in tissue engineering applications.
37230308	2	0	theme	0.2	504:506	arg1	%					507:507	%	507:507	%	507:507	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	8	1	theme	rate	1706:1709	arg1	analysis					1652:1659	Binomial fit analysis	1639:1659	Binomial fit analysis of weight gain rate and specific weight gain rate	1639:1709	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	9	2	theme	antioxidant	1887:1897	arg1	capacity					1899:1906	antioxidant capacity	1887:1906	antioxidant capacity	1887:1906	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	2	theme	antioxidant	1887:1897	arg1	prawns					1860:1865	juvenile prawns	1851:1865	juvenile prawns growth performance, antioxidant capacity, and non-specific immunity	1851:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	5	3	theme	capacity	1033:1040	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	3	theme	capacity	1033:1040	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	3	4	theme	specific	587:594	arg1	rate					608:611	specific weight gain rate	587:611	specific weight gain rate	587:611	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	8	5	theme	Binomial	1639:1646	arg1	analysis					1652:1659	Binomial fit analysis	1639:1659	Binomial fit analysis of weight gain rate and specific weight gain rate	1639:1709	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	5	6	theme	prawns	1192:1197	arg1	hepatopancreas					1165:1178	the hepatopancreas	1161:1178	the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	1161:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	1	7	theme	tissue	250:255	arg1	structure					257:265	hepatopancreas tissue structure	235:265	hepatopancreas tissue structure	235:265	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	7	8	theme	PCR	1538:1540	arg1	results					1504:1510	The results	1500:1510	The results of real-time quantitative PCR	1500:1540	The results of real-time quantitative PCR indicated that dietary β-1,3-glucan promoted expression of antioxidant and immune-related genes.
37230308	2	9	theme	curdlan	509:515	arg1	contents					449:456	different contents	439:456	different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan	439:515	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	7	10	theme	real-time	1515:1523	arg1	PCR					1538:1540	real-time quantitative PCR	1515:1540	real-time quantitative PCR	1515:1540	The results of real-time quantitative PCR indicated that dietary β-1,3-glucan promoted expression of antioxidant and immune-related genes.
37230308	5	11	theme	dietary	1366:1372	arg1	β-1,3-glucan					1374:1385	dietary β-1,3-glucan	1366:1385	dietary β-1,3-glucan	1366:1385	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	8	12	theme	weight	1664:1669	arg1	rate					1676:1679	weight gain rate	1664:1679	weight gain rate	1664:1679	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	3	13	theme	gain	603:606	arg1	rate					608:611	specific weight gain rate	587:611	specific weight gain rate	587:611	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	8	14	theme	rate	1676:1679	arg1	analysis					1652:1659	Binomial fit analysis	1639:1659	Binomial fit analysis of weight gain rate and specific weight gain rate	1639:1709	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	1	15	theme	β-1,3-glucan	177:188	arg1	effects					158:164	The effects	154:164	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense)	154:356	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	9	16	theme	healthy	1971:1977	arg1	culture					1979:1985	shrimp healthy culture	1964:1985	shrimp healthy culture	1964:1985	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	17	theme	shrimp	1964:1969	arg1	culture					1979:1985	shrimp healthy culture	1964:1985	shrimp healthy culture	1964:1985	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	0	18	theme	river	114:118	arg1	prawn					120:124	the river prawn	110:124	the river prawn (Macrobrachium nipponense)	110:151	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	0	18	theme	river	114:118	arg1	nipponense					141:150	Macrobrachium nipponense	127:150	Macrobrachium nipponense	127:150	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	8	19	theme	gain	1701:1704	arg1	rate					1706:1709	specific weight gain rate	1685:1709	specific weight gain rate	1685:1709	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	6	20	theme	malondialdehyde	1400:1414	arg1	content					1422:1428	The highest malondialdehyde (MDA) content	1388:1428	The highest malondialdehyde (MDA) content	1388:1428	The highest malondialdehyde (MDA) content was observed in juvenile prawns without β-1,3-glucan supplementation.
37230308	3	21	theme	juvenile	659:666	arg1	prawns					668:673	juvenile prawns	659:673	juvenile prawns fed 0.2% β-1,3-glucan	659:695	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	1	22	from	effects	158:164	arg1	structure					257:265	hepatopancreas tissue structure	235:265	hepatopancreas tissue structure	235:265	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	22	from	effects	158:164	arg1	activities					280:289	antioxidant activities	268:289	antioxidant activities	268:289	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	22	from	effects	158:164	arg1	performance					204:214	growth performance	197:214	growth performance	197:214	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	22	from	effects	158:164	arg1	composition					222:232	body composition	217:232	body composition	217:232	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	22	from	effects	158:164	arg1	response					303:310	immune response	296:310	immune response	296:310	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	2	23	theme	β-1,3-glucan	461:472	arg1	contents					449:456	different contents	439:456	different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan	439:515	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	4	24	theme	prawns	821:826	arg1	content					810:816	The whole-body crude lipid content	783:816	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan	783:869	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	4	24	theme	prawns	821:826	arg1	higher					889:894	higher	889:894	higher	889:894	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	3	25	theme	%	682:682	arg1	β-1,3-glucan					684:695	0.2% β-1,3-glucan	679:695	0.2% β-1,3-glucan	679:695	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	0	26	theme	antioxidant	67:77	arg1	status					79:84	antioxidant status	67:84	antioxidant status	67:84	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	1	27	theme	river	319:323	arg1	prawn					325:329	the river prawn	315:329	the river prawn (Macrobrachium nipponense)	315:356	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	27	theme	river	319:323	arg1	nipponense					346:355	Macrobrachium nipponense	332:355	Macrobrachium nipponense	332:355	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	0	28	from	Effects	0:6	arg1	performance					54:64	growth performance	47:64	growth performance	47:64	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	0	28	from	Effects	0:6	arg1	status					79:84	antioxidant status	67:84	antioxidant status	67:84	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	0	28	from	Effects	0:6	arg1	response					98:105	immune response	91:105	immune response	91:105	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	5	29	theme	lysozyme	1067:1074	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	29	theme	lysozyme	1067:1074	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	9	30	dep	prawns	1860:1865	arg1	capacity					1899:1906	antioxidant capacity	1887:1906	antioxidant capacity	1887:1906	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	30	dep	prawns	1860:1865	arg1	prawns					1860:1865	juvenile prawns	1851:1865	juvenile prawns growth performance, antioxidant capacity, and non-specific immunity	1851:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	30	dep	prawns	1860:1865	arg1	immunity					1926:1933	non-specific immunity	1913:1933	non-specific immunity	1913:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	30	dep	prawns	1860:1865	arg1	performance					1874:1884	growth performance	1867:1884	growth performance	1867:1884	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	4	31	theme	crude	798:802	arg1	content					810:816	The whole-body crude lipid content	783:816	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan	783:869	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	4	31	theme	crude	798:802	arg1	higher					889:894	higher	889:894	higher	889:894	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	6	32	theme	juvenile	1446:1453	arg1	prawns					1455:1460	juvenile prawns	1446:1460	juvenile prawns without β-1,3-glucan supplementation	1446:1497	The highest malondialdehyde (MDA) content was observed in juvenile prawns without β-1,3-glucan supplementation.
37230308	5	33	theme	0.2	1203:1205	arg1	%					1206:1206	%	1206:1206	%	1206:1206	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	34	theme	acid	1103:1106	arg1	ACP					1121:1123	ACP	1121:1123	ACP	1121:1123	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	34	theme	acid	1103:1106	arg1	phosphatase					1108:1118	acid phosphatase	1103:1118	acid phosphatase (ACP)	1103:1124	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	0	35	theme	immune	91:96	arg1	response					98:105	immune response	91:105	immune response	91:105	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	3	36	theme	β-1,3-glucan	741:752	arg1	%					739:739	0% β-1,3-glucan	738:752	0% β-1,3-glucan	738:752	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	9	37	theme	non-specific	1913:1924	arg1	prawns					1860:1865	juvenile prawns	1851:1865	juvenile prawns growth performance, antioxidant capacity, and non-specific immunity	1851:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	37	theme	non-specific	1913:1924	arg1	immunity					1926:1933	non-specific immunity	1913:1933	non-specific immunity	1913:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	8	38	theme	0.550	1783:1787	arg1	%					1795:1795	0.550%-0.553%	1783:1795	0.550%-0.553%	1783:1795	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	8	38	theme	0.550	1783:1787	arg1	requirement					1748:1758	the optimum β-1,3-glucan requirement	1723:1758	the optimum β-1,3-glucan requirement of juvenile prawns	1723:1777	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	9	39	theme	dietary	1821:1827	arg1	β-1,3-glucan					1829:1840	suitable dietary β-1,3-glucan	1812:1840	suitable dietary β-1,3-glucan	1812:1840	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	5	40	theme	0.2	1277:1279	arg1	%					1280:1280	%	1280:1280	%	1280:1280	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	2	41	theme	juvenile	391:398	arg1	prawns					400:405	900 juvenile prawns	387:405	900 juvenile prawns	387:405	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	0	42	theme	dietary	11:17	arg1	β-1,3-glucan					27:38	dietary soluble β-1,3-glucan	11:38	dietary soluble β-1,3-glucan	11:38	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	1	43	theme	antioxidant	268:278	arg1	activities					280:289	antioxidant activities	268:289	antioxidant activities	268:289	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	0	44	dep	performance	54:64	arg1	the					43:45	the	43:45	the	43:45	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	5	45	theme	curdlan	1282:1288	arg1	p < 0.05					1298:1305	p < 0.05	1298:1305	p < 0.05	1298:1305	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	45	theme	curdlan	1282:1288	arg1	groups					1290:1295	0.2% curdlan groups	1277:1295	0.2% curdlan groups (p < 0.05)	1277:1306	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	3	46	theme	growth	534:539	arg1	higher					716:721	higher	716:721	higher	716:721	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	3	46	theme	growth	534:539	arg1	rate					541:544	The growth rate	530:544	The growth rate	530:544	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	5	47	from	activities	973:982	arg1	hepatopancreas					1165:1178	the hepatopancreas	1161:1178	the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	1161:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	0	48	theme	β-1,3-glucan	27:38	arg1	Effects					0:6	Effects	0:6	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).	0:152	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	5	49	theme	phosphatase	1140:1150	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	49	theme	phosphatase	1140:1150	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	50	theme	dismutase	998:1006	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	50	theme	dismutase	998:1006	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	3	51	theme	gain	554:557	arg1	rate					559:562	weight gain rate	547:562	weight gain rate	547:562	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	1	52	theme	growth	197:202	arg1	performance					204:214	growth performance	197:214	growth performance	197:214	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	5	53	theme	catalase	1051:1058	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	53	theme	catalase	1051:1058	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	7	54	theme	dietary	1557:1563	arg1	β-1,3-glucan					1565:1576	dietary β-1,3-glucan	1557:1576	dietary β-1,3-glucan	1557:1576	The results of real-time quantitative PCR indicated that dietary β-1,3-glucan promoted expression of antioxidant and immune-related genes.
37230308	3	55	theme	growth	574:579	arg1	rate					581:584	specific growth rate	565:584	specific growth rate	565:584	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	5	56	theme	antioxidant	1021:1031	arg1	T-AOC					1043:1047	T-AOC	1043:1047	T-AOC	1043:1047	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	56	theme	antioxidant	1021:1031	arg1	capacity					1033:1040	total antioxidant capacity	1015:1040	total antioxidant capacity (T-AOC)	1015:1048	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	8	57	theme	fit	1648:1650	arg1	analysis					1652:1659	Binomial fit analysis	1639:1659	Binomial fit analysis of weight gain rate and specific weight gain rate	1639:1709	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	5	58	theme	juvenile	1183:1190	arg1	prawns					1192:1197	juvenile prawns	1183:1197	juvenile prawns fed 0.2% β-1,3-glucan	1183:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	1	59	theme	hepatopancreas	235:248	arg1	structure					257:265	hepatopancreas tissue structure	235:265	hepatopancreas tissue structure	235:265	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	7	60	theme	quantitative	1525:1536	arg1	PCR					1538:1540	real-time quantitative PCR	1515:1540	real-time quantitative PCR	1515:1540	The results of real-time quantitative PCR indicated that dietary β-1,3-glucan promoted expression of antioxidant and immune-related genes.
37230308	3	61	theme	weight	596:601	arg1	rate					608:611	specific weight gain rate	587:611	specific weight gain rate	587:611	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	1	62	theme	immune	296:301	arg1	response					303:310	immune response	296:310	immune response	296:310	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	8	63	theme	β-1,3-glucan	1735:1746	arg1	requirement					1748:1758	the optimum β-1,3-glucan requirement	1723:1758	the optimum β-1,3-glucan requirement of juvenile prawns	1723:1777	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	8	63	theme	β-1,3-glucan	1735:1746	arg1	%					1795:1795	0.550%-0.553%	1783:1795	0.550%-0.553%	1783:1795	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	8	64	theme	gain	1671:1674	arg1	rate					1676:1679	weight gain rate	1664:1679	weight gain rate	1664:1679	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	5	65	theme	enzyme	966:971	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	65	theme	enzyme	966:971	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	2	66	theme	%	507:507	arg1	curdlan					509:515	0.2% curdlan	504:515	0.2% curdlan	504:515	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	9	67	theme	juvenile	1851:1858	arg1	capacity					1899:1906	antioxidant capacity	1887:1906	antioxidant capacity	1887:1906	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	67	theme	juvenile	1851:1858	arg1	prawns					1860:1865	juvenile prawns	1851:1865	juvenile prawns growth performance, antioxidant capacity, and non-specific immunity	1851:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	67	theme	juvenile	1851:1858	arg1	immunity					1926:1933	non-specific immunity	1913:1933	non-specific immunity	1913:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	67	theme	juvenile	1851:1858	arg1	performance					1874:1884	growth performance	1867:1884	growth performance	1867:1884	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	3	68	theme	condition	614:622	arg1	factor					624:629	condition factor	614:629	condition factor	614:629	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	1	69	theme	dietary	169:175	arg1	β-1,3-glucan					177:188	dietary β-1,3-glucan	169:188	dietary β-1,3-glucan	169:188	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	0	70	theme	prawn	120:124	arg1	performance					54:64	growth performance	47:64	growth performance	47:64	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	0	70	theme	prawn	120:124	arg1	status					79:84	antioxidant status	67:84	antioxidant status	67:84	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	0	70	theme	prawn	120:124	arg1	response					98:105	immune response	91:105	immune response	91:105	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	3	71	theme	hepatosomatic	636:648	arg1	index					650:654	hepatosomatic index	636:654	hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan	636:695	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	0	72	theme	Macrobrachium	127:139	arg1	prawn					120:124	the river prawn	110:124	the river prawn (Macrobrachium nipponense)	110:151	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	0	72	theme	Macrobrachium	127:139	arg1	nipponense					141:150	Macrobrachium nipponense	127:150	Macrobrachium nipponense	127:150	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	2	73	dep	β-1,3-glucan	461:472	arg1	%					498:498	1.0%	495:498	1.0%	495:498	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	2	73	dep	β-1,3-glucan	461:472	arg1	%					476:476	0%	475:476	0%	475:476	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	2	73	dep	β-1,3-glucan	461:472	arg1	%					482:482	0.1%	479:482	0.1%	479:482	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	2	73	dep	β-1,3-glucan	461:472	arg1	%					488:488	0.2%	485:488	0.2%	485:488	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	3	74	dep	%	761:761	arg1	curdlan					763:769	curdlan (p < 0.05)	763:780	0.2% curdlan (p < 0.05)	758:780	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	6	75	theme	highest	1392:1398	arg1	content					1422:1428	The highest malondialdehyde (MDA) content	1388:1428	The highest malondialdehyde (MDA) content	1388:1428	The highest malondialdehyde (MDA) content was observed in juvenile prawns without β-1,3-glucan supplementation.
37230308	3	76	theme	prawns	668:673	arg1	factor					624:629	condition factor	614:629	condition factor	614:629	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	3	76	theme	prawns	668:673	arg1	higher					716:721	higher	716:721	higher	716:721	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	3	76	theme	prawns	668:673	arg1	rate					559:562	weight gain rate	547:562	weight gain rate	547:562	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	3	76	theme	prawns	668:673	arg1	rate					608:611	specific weight gain rate	587:611	specific weight gain rate	587:611	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	3	76	theme	prawns	668:673	arg1	rate					541:544	The growth rate	530:544	The growth rate	530:544	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	3	76	theme	prawns	668:673	arg1	index					650:654	hepatosomatic index	636:654	hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan	636:695	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	3	76	theme	prawns	668:673	arg1	rate					581:584	specific growth rate	565:584	specific growth rate	565:584	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	8	77	theme	optimum	1727:1733	arg1	requirement					1748:1758	the optimum β-1,3-glucan requirement	1723:1758	the optimum β-1,3-glucan requirement of juvenile prawns	1723:1777	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	8	77	theme	optimum	1727:1733	arg1	%					1795:1795	0.550%-0.553%	1783:1795	0.550%-0.553%	1783:1795	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	4	78	theme	lipid	804:808	arg1	content					810:816	The whole-body crude lipid content	783:816	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan	783:869	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	4	78	theme	lipid	804:808	arg1	higher					889:894	higher	889:894	higher	889:894	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	5	79	theme	immune	959:964	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	79	theme	immune	959:964	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	2	80	theme	different	439:447	arg1	contents					449:456	different contents	439:456	different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan	439:515	In total, 900 juvenile prawns were fed one of five diets with different contents of β-1,3-glucan (0%, 0.1%, 0.2%, and 1.0%) or 0.2% curdlan for 6 weeks.
37230308	6	81	theme	β-1,3-glucan	1470:1481	arg1	supplementation					1483:1497	β-1,3-glucan supplementation	1470:1497	β-1,3-glucan supplementation	1470:1497	The highest malondialdehyde (MDA) content was observed in juvenile prawns without β-1,3-glucan supplementation.
37230308	8	82	theme	juvenile	1763:1770	arg1	prawns					1772:1777	juvenile prawns	1763:1777	juvenile prawns	1763:1777	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	1	83	theme	prawn	325:329	arg1	structure					257:265	hepatopancreas tissue structure	235:265	hepatopancreas tissue structure	235:265	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	83	theme	prawn	325:329	arg1	activities					280:289	antioxidant activities	268:289	antioxidant activities	268:289	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	83	theme	prawn	325:329	arg1	performance					204:214	growth performance	197:214	growth performance	197:214	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	83	theme	prawn	325:329	arg1	composition					222:232	body composition	217:232	body composition	217:232	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	83	theme	prawn	325:329	arg1	response					303:310	immune response	296:310	immune response	296:310	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	3	84	theme	0.2	679:681	arg1	%					682:682	%	682:682	%	682:682	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	1	85	theme	Macrobrachium	332:344	arg1	prawn					325:329	the river prawn	315:329	the river prawn (Macrobrachium nipponense)	315:356	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	1	85	theme	Macrobrachium	332:344	arg1	nipponense					346:355	Macrobrachium nipponense	332:355	Macrobrachium nipponense	332:355	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
37230308	4	86	theme	whole-body	787:796	arg1	content					810:816	The whole-body crude lipid content	783:816	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan	783:869	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	4	86	theme	whole-body	787:796	arg1	higher					889:894	higher	889:894	higher	889:894	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	5	87	theme	%	1206:1206	arg1	β-1,3-glucan					1208:1219	0.2% β-1,3-glucan	1203:1219	0.2% β-1,3-glucan	1203:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	88	theme	phosphatase	1108:1118	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	88	theme	phosphatase	1108:1118	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	8	89	theme	%	1788:1788	arg1	%					1795:1795	0.550%-0.553%	1783:1795	0.550%-0.553%	1783:1795	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	8	89	theme	%	1788:1788	arg1	requirement					1748:1758	the optimum β-1,3-glucan requirement	1723:1758	the optimum β-1,3-glucan requirement of juvenile prawns	1723:1777	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	5	90	theme	antioxidant	943:953	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	90	theme	antioxidant	943:953	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	6	91	located	observed	1434:1441	arg2	content					1422:1428	The highest malondialdehyde (MDA) content	1388:1428	The highest malondialdehyde (MDA) content	1388:1428	The highest malondialdehyde (MDA) content was observed in juvenile prawns without β-1,3-glucan supplementation.
37230308	6	91	located	observed	1434:1441	arg1	prawns					1455:1460	juvenile prawns	1446:1460	juvenile prawns without β-1,3-glucan supplementation	1446:1497	The highest malondialdehyde (MDA) content was observed in juvenile prawns without β-1,3-glucan supplementation.
37230308	8	92	theme	weight	1694:1699	arg1	rate					1706:1709	specific weight gain rate	1685:1709	specific weight gain rate	1685:1709	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	5	93	theme	phenoloxidase	1083:1095	arg1	higher					1240:1245	higher	1240:1245	higher	1240:1245	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	93	theme	phenoloxidase	1083:1095	arg1	activities					973:982	The antioxidant and immune enzyme activities	939:982	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan	939:1219	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	0	94	theme	soluble	19:25	arg1	β-1,3-glucan					27:38	dietary soluble β-1,3-glucan	11:38	dietary soluble β-1,3-glucan	11:38	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	5	95	theme	%	1280:1280	arg1	p < 0.05					1298:1305	p < 0.05	1298:1305	p < 0.05	1298:1305	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	95	theme	%	1280:1280	arg1	groups					1290:1295	0.2% curdlan groups	1277:1295	0.2% curdlan groups (p < 0.05)	1277:1306	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	9	96	theme	suitable	1812:1819	arg1	β-1,3-glucan					1829:1840	suitable dietary β-1,3-glucan	1812:1840	suitable dietary β-1,3-glucan	1812:1840	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	5	97	theme	total	1015:1019	arg1	T-AOC					1043:1047	T-AOC	1043:1047	T-AOC	1043:1047	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	97	theme	total	1015:1019	arg1	capacity					1033:1040	total antioxidant capacity	1015:1040	total antioxidant capacity (T-AOC)	1015:1048	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	98	theme	alkaline	1131:1138	arg1	phosphatase					1140:1150	alkaline phosphatase	1131:1150	alkaline phosphatase	1131:1150	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	8	99	theme	prawns	1772:1777	arg1	requirement					1748:1758	the optimum β-1,3-glucan requirement	1723:1758	the optimum β-1,3-glucan requirement of juvenile prawns	1723:1777	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	8	99	theme	prawns	1772:1777	arg1	%					1795:1795	0.550%-0.553%	1783:1795	0.550%-0.553%	1783:1795	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	0	100	theme	growth	47:52	arg1	performance					54:64	growth performance	47:64	growth performance	47:64	Effects of dietary soluble β-1,3-glucan on the growth performance, antioxidant status, and immune response of the river prawn (Macrobrachium nipponense).
37230308	5	101	theme	superoxide	987:996	arg1	dismutase					998:1006	superoxide dismutase	987:1006	superoxide dismutase (SOD)	987:1012	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	101	theme	superoxide	987:996	arg1	SOD					1009:1011	SOD	1009:1011	SOD	1009:1011	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	5	101	theme	superoxide	987:996	arg1	AKP					1153:1155	AKP	1153:1155	AKP	1153:1155	The antioxidant and immune enzyme activities of superoxide dismutase (SOD), total antioxidant capacity (T-AOC), catalase (CAT), lysozyme (LZM), phenoloxidase (PO), acid phosphatase (ACP), and alkaline phosphatase (AKP) in the hepatopancreas of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those of the control and 0.2% curdlan groups (p < 0.05), and tended to increase and then decrease with increasing dietary β-1,3-glucan.
37230308	7	102	theme	immune-related	1617:1630	arg1	genes					1632:1636	immune-related genes	1617:1636	immune-related genes	1617:1636	The results of real-time quantitative PCR indicated that dietary β-1,3-glucan promoted expression of antioxidant and immune-related genes.
37230308	8	103	theme	specific	1685:1692	arg1	rate					1706:1709	specific weight gain rate	1685:1709	specific weight gain rate	1685:1709	Binomial fit analysis of weight gain rate and specific weight gain rate showed that the optimum β-1,3-glucan requirement of juvenile prawns was 0.550%-0.553%.
37230308	3	104	theme	weight	547:552	arg1	rate					559:562	weight gain rate	547:562	weight gain rate	547:562	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	7	105	theme	genes	1632:1636	arg1	expression					1587:1596	expression	1587:1596	expression of antioxidant and immune-related genes	1587:1636	The results of real-time quantitative PCR indicated that dietary β-1,3-glucan promoted expression of antioxidant and immune-related genes.
37230308	7	106	theme	antioxidant	1601:1611	arg1	expression					1587:1596	expression	1587:1596	expression of antioxidant and immune-related genes	1587:1636	The results of real-time quantitative PCR indicated that dietary β-1,3-glucan promoted expression of antioxidant and immune-related genes.
37230308	4	107	theme	control	913:919	arg1	group					921:925	the control group	909:925	the control group (p < 0.05)	909:936	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	4	107	theme	control	913:919	arg1	p < 0.05					928:935	p < 0.05	928:935	p < 0.05	928:935	The whole-body crude lipid content of prawns supplemented with curdlan and β-1,3-glucan was significantly higher than that of the control group (p < 0.05).
37230308	9	108	theme	growth	1867:1872	arg1	prawns					1860:1865	juvenile prawns	1851:1865	juvenile prawns growth performance, antioxidant capacity, and non-specific immunity	1851:1933	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	9	108	theme	growth	1867:1872	arg1	performance					1874:1884	growth performance	1867:1884	growth performance	1867:1884	We found that suitable dietary β-1,3-glucan improved juvenile prawns growth performance, antioxidant capacity, and non-specific immunity, which provide reference for shrimp healthy culture.
37230308	3	109	theme	specific	565:572	arg1	rate					581:584	specific growth rate	565:584	specific growth rate	565:584	The growth rate, weight gain rate, specific growth rate, specific weight gain rate, condition factor, and hepatosomatic index of juvenile prawns fed 0.2% β-1,3-glucan were significantly higher than those fed 0% β-1,3-glucan and 0.2% curdlan (p < 0.05).
37230308	1	110	theme	body	217:220	arg1	composition					222:232	body composition	217:232	body composition	217:232	The effects of dietary β-1,3-glucan on the growth performance, body composition, hepatopancreas tissue structure, antioxidant activities, and immune response of the river prawn (Macrobrachium nipponense) were investigated.
35929272	8	0	theme	Phalloidin/DAPI	2134:2148	arg1	staining					2150:2157	Phalloidin/DAPI staining	2134:2157	Phalloidin/DAPI staining	2134:2157	Furthermore, Live/Dead assay and Phalloidin/DAPI staining, to assess the cytoskeletal organization, proved that the hydrogels can provide a suitable microenvironment for cell adhesion, viability, and proliferation, after being encapsulated.
35929272	6	1	theme	%	1745:1745	arg1	chitosan					1747:1754	3% chitosan	1744:1754	3% chitosan	1744:1754	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	9	2	theme	hydrogel	2429:2436	arg1	formulations					2438:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	3	3	from	solutions	860:868	arg1	solutions					815:823	solutions	815:823	solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae	815:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	3	from	solutions	860:868	arg1	chitosan					831:838	3% chitosan	828:838	3% chitosan from squid pens and solutions of 10% fucoidan from brown algae	828:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	4	theme	collagen	745:752	arg1	solutions					729:737	solutions	729:737	solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin	729:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	1	5	theme	biomedical	272:281	arg1	sector					283:288	the biomedical sector	268:288	the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM)	268:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	3	6	theme	5	742:742	arg1	%					743:743	%	743:743	%	743:743	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	6	7	theme	water	1401:1405	arg1	ability					1414:1420	water uptake ability	1401:1420	water uptake ability	1401:1420	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	9	8	theme	good	2461:2464	arg1	architecture					2477:2488	a good structural architecture	2459:2488	a good structural architecture	2459:2488	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	8	9	theme	cytoskeletal	2174:2185	arg1	organization					2187:2198	the cytoskeletal organization	2170:2198	the cytoskeletal organization	2170:2198	Furthermore, Live/Dead assay and Phalloidin/DAPI staining, to assess the cytoskeletal organization, proved that the hydrogels can provide a suitable microenvironment for cell adhesion, viability, and proliferation, after being encapsulated.
35929272	6	10	theme	degradation	1784:1794	arg1	test					1796:1799	the degradation test	1780:1799	the degradation test	1780:1799	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	11	theme	3	776:776	arg1	%					777:777	%	777:777	%	777:777	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	12	from	pens	851:854	arg1	solutions					815:823	solutions	815:823	solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae	815:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	12	from	pens	851:854	arg1	chitosan					831:838	3% chitosan	828:838	3% chitosan from squid pens and solutions of 10% fucoidan from brown algae	828:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	6	13	theme	fucoidan	1767:1774	arg1	collagen					1718:1725	3% collagen	1715:1725	3% collagen from skin shark	1715:1741	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	13	theme	fucoidan	1767:1774	arg1	fucoidan					1767:1774	fucoidan	1767:1774	fucoidan	1767:1774	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	13	theme	fucoidan	1767:1774	arg1	biopolymers					1668:1678	all biopolymers	1664:1678	all biopolymers	1664:1678	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	13	theme	fucoidan	1767:1774	arg1	chitosan					1747:1754	3% chitosan	1744:1754	3% chitosan	1744:1754	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	13	theme	fucoidan	1767:1774	arg1	%					1762:1762	10%	1760:1762	10% of fucoidan	1760:1774	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	9	14	theme	H3	2529:2530	arg1	biomaterial					2532:2542	the H3 biomaterial	2525:2542	the H3 biomaterial due to containing more polymers in their composition	2525:2595	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	3	15	theme	blue	793:796	arg1	skin					804:807	blue shark skin	793:807	blue shark skin	793:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	6	16	from	jellyfish	1704:1712	arg1	collagen					1690:1697	5% collagen	1687:1697	5% collagen from jellyfish	1687:1712	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	17	theme	collagen	779:786	arg1	solutions					729:737	solutions	729:737	solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin	729:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	5	18	contain	contain	1232:1238	arg1	collagens					1217:1225	The studied collagens	1205:1225	The studied collagens	1205:1225	The studied collagens also contain a denaturation temperature of around 30-32 °C and a molecular weight between 120 and 125 kDa.
35929272	5	18	contain	contain	1232:1238	arg2	temperature					1255:1265	a denaturation temperature	1240:1265	a denaturation temperature of around 30-32 °C and a molecular weight between 120 and 125 kDa	1240:1331	The studied collagens also contain a denaturation temperature of around 30-32 °C and a molecular weight between 120 and 125 kDa.
35929272	6	19	dep	mechanical	1511:1520	arg1	character					1543:1551	strong viscoelastic character	1523:1551	strong viscoelastic character	1523:1551	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	20	theme	strong	1523:1528	arg1	character					1543:1551	strong viscoelastic character	1523:1551	strong viscoelastic character	1523:1551	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	1	21	theme	recent	145:150	arg1	decade					152:157	the recent decade	141:157	the recent decade	141:157	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	9	22	theme	due	2544:2546	arg1	biomaterial					2532:2542	the H3 biomaterial	2525:2542	the H3 biomaterial due to containing more polymers in their composition	2525:2595	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	8	23	theme	suitable	2241:2248	arg1	microenvironment					2250:2265	a suitable microenvironment	2239:2265	a suitable microenvironment for cell adhesion, viability, and proliferation	2239:2313	Furthermore, Live/Dead assay and Phalloidin/DAPI staining, to assess the cytoskeletal organization, proved that the hydrogels can provide a suitable microenvironment for cell adhesion, viability, and proliferation, after being encapsulated.
35929272	4	24	theme	triple	1111:1116	arg1	structure					1126:1134	the triple helical structure	1107:1134	the triple helical structure	1107:1134	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	6	25	theme	mass	1815:1818	arg1	loss					1820:1823	a mass loss	1813:1823	a mass loss around 18%	1813:1834	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	1	26	theme	Tissue	335:340	arg1	Engineering					342:352	Tissue Engineering	335:352	Tissue Engineering	335:352	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	7	27	dep	in	1950:1951	arg1	vitro					1953:1957	vitro	1953:1957	vitro	1953:1957	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	6	28	theme	mechanical	1511:1520	arg1	properties					1579:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	29	theme	10	873:874	arg1	%					875:875	%	875:875	%	875:875	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	4	30	theme	Acid	990:993	arg1	ATR-FTIR					1005:1012	ATR-FTIR	1005:1012	ATR-FTIR	1005:1012	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	30	theme	Acid	990:993	arg1	ICP					1029:1031	ICP	1029:1031	ICP	1029:1031	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	30	theme	Acid	990:993	arg1	XRD					1034:1036	XRD	1034:1036	XRD	1034:1036	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	30	theme	Acid	990:993	arg1	CD					1015:1016	CD	1015:1016	CD	1015:1016	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	30	theme	Acid	990:993	arg1	SDS-PAGE					1019:1026	SDS-PAGE	1019:1026	SDS-PAGE	1019:1026	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	30	theme	Acid	990:993	arg1	analysis					995:1002	Amino Acid analysis	984:1002	Amino Acid analysis	984:1002	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	6	31	theme	%	1688:1688	arg1	collagen					1690:1697	5% collagen	1687:1697	5% collagen from jellyfish	1687:1712	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	5	32	theme	studied	1209:1215	arg1	collagens					1217:1225	The studied collagens	1205:1225	The studied collagens	1205:1225	The studied collagens also contain a denaturation temperature of around 30-32 °C and a molecular weight between 120 and 125 kDa.
35929272	3	33	theme	%	829:829	arg1	chitosan					831:838	3% chitosan	828:838	3% chitosan from squid pens and solutions of 10% fucoidan from brown algae	828:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	0	34	theme	cell-laden	77:86	arg1	hydrogel					88:95	cell-laden hydrogel	77:95	cell-laden hydrogel envisaging cartilage tissue engineering	77:135	Marine origin biomaterials using a compressive and absorption methodology as cell-laden hydrogel envisaging cartilage tissue engineering.
35929272	1	35	theme	origin	167:172	arg1	products					174:181	marine origin products	160:181	marine origin products	160:181	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	1	35	theme	origin	167:172	arg1	blocks					223:228	building blocks	214:228	building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM)	214:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	6	36	contain	had	1495:1497	arg1	formulations					1482:1493	all formulations	1478:1493	all formulations	1478:1493	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	36	contain	had	1495:1497	arg2	properties					1579:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	37	theme	brown	891:895	arg1	algae					897:901	brown algae	891:901	brown algae	891:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	4	38	theme	physico-chemical	940:955	arg1	characterization					957:972	The biopolymer physico-chemical characterization	925:972	The biopolymer physico-chemical characterization	925:972	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	3	39	from	jellyfish	759:767	arg1	solutions					729:737	solutions	729:737	solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin	729:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	39	from	jellyfish	759:767	arg1	collagen					745:752	5% collagen	742:752	5% collagen from jellyfish	742:767	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	7	40	theme	hydrogel	2052:2059	arg1	formulations					2061:2072	all hydrogel formulations	2048:2072	all hydrogel formulations	2048:2072	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	5	41	theme	molecular	1292:1300	arg1	weight					1302:1307	a molecular weight	1290:1307	a molecular weight between 120 and 125 kDa	1290:1331	The studied collagens also contain a denaturation temperature of around 30-32 °C and a molecular weight between 120 and 125 kDa.
35929272	0	42	theme	tissue	118:123	arg1	engineering					125:135	cartilage tissue engineering	108:135	cartilage tissue engineering	108:135	Marine origin biomaterials using a compressive and absorption methodology as cell-laden hydrogel envisaging cartilage tissue engineering.
35929272	3	43	theme	fucoidan	877:884	arg1	pens					851:854	squid pens	845:854	squid pens	845:854	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	43	theme	fucoidan	877:884	arg1	solutions					860:868	solutions	860:868	solutions of 10% fucoidan from brown algae	860:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	0	44	theme	Marine	0:5	arg1	biomaterials					14:25	Marine origin biomaterials	0:25	Marine origin biomaterials	0:25	Marine origin biomaterials using a compressive and absorption methodology as cell-laden hydrogel envisaging cartilage tissue engineering.
35929272	1	45	theme	sector	283:288	arg1	demand					258:263	the constant demand	245:263	the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM)	245:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	6	46	theme	stability	1569:1577	arg1	properties					1579:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	47	theme	different	907:915	arg1	ratios					917:922	different ratios	907:922	different ratios	907:922	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	2	48	theme	collagen-chitosan-fucoidan	433:458	arg1	hydrogel					460:467	marine collagen-chitosan-fucoidan hydrogel	426:467	marine collagen-chitosan-fucoidan hydrogel	426:467	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	4	49	theme	type	1173:1176	arg1	collagen					1181:1188	type II collagen	1173:1188	type II collagen	1173:1188	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	6	50	dep	positive	1610:1617	arg1	highlight					1619:1627	highlight	1619:1627	highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively	1619:1929	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	51	theme	%	1716:1716	arg1	collagen					1718:1725	3% collagen	1715:1725	3% collagen from skin shark	1715:1741	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	7	52	theme	encapsulated	2015:2026	arg1	state					2028:2032	encapsulated state	2015:2032	encapsulated state	2015:2032	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	2	53	theme	several	402:408	arg1	combinations					410:421	several combinations	402:421	several combinations of marine collagen-chitosan-fucoidan hydrogel	402:467	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	9	54	theme	articular	2632:2640	arg1	therapies					2652:2660	biomedical articular cartilage therapies	2621:2660	biomedical articular cartilage therapies	2621:2660	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	3	55	from	skin	804:807	arg1	solutions					729:737	solutions	729:737	solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin	729:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	55	from	skin	804:807	arg1	collagen					779:786	3% collagen	776:786	3% collagen from blue shark skin	776:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	1	56	theme	devices	323:329	arg1	development					304:314	the development	300:314	the development of new devices for Tissue Engineering and Regenerative Medicine (TERM)	300:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	8	57	theme	Live/Dead	2114:2122	arg1	assay					2124:2128	Live/Dead assay	2114:2128	Live/Dead assay	2114:2128	Furthermore, Live/Dead assay and Phalloidin/DAPI staining, to assess the cytoskeletal organization, proved that the hydrogels can provide a suitable microenvironment for cell adhesion, viability, and proliferation, after being encapsulated.
35929272	2	58	dep	compressive	518:528	arg1	developed					495:503	developed	495:503	developed	495:503	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	7	59	theme	chondrocyte	1986:1996	arg1	ATDC5					2005:2009	ATDC5	2005:2009	ATDC5	2005:2009	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	7	59	theme	chondrocyte	1986:1996	arg1	cells					1998:2002	chondrocyte cells	1986:2002	chondrocyte cells (ATDC5)	1986:2010	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	9	60	theme	collagen	2384:2391	arg1	formulations					2438:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	9	61	theme	jellyfish/shark	2394:2408	arg1	formulations					2438:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	6	62	theme	degradation	1423:1433	arg1	rate					1435:1438	degradation rate	1423:1438	degradation rate	1423:1438	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	9	63	theme	-chitosan-fucoidan	2410:2427	arg1	formulations					2438:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	2	64	theme	biopolymers	620:630	arg1	solution					632:639	compacting the biopolymers solution	605:639	compacting the biopolymers solution	605:639	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	6	65	theme	3	1744:1744	arg1	%					1745:1745	%	1745:1745	%	1745:1745	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	2	66	theme	compacting	605:614	arg1	solution					632:639	compacting the biopolymers solution	605:639	compacting the biopolymers solution	605:639	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	9	67	theme	more	2562:2565	arg1	polymers					2567:2574	more polymers	2562:2574	more polymers	2562:2574	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	6	68	from	shark	1737:1741	arg1	collagen					1718:1725	3% collagen	1715:1725	3% collagen from skin shark	1715:1741	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	68	from	shark	1737:1741	arg1	fucoidan					1767:1774	fucoidan	1767:1774	fucoidan	1767:1774	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	68	from	shark	1737:1741	arg1	biopolymers					1668:1678	all biopolymers	1664:1678	all biopolymers	1664:1678	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	68	from	shark	1737:1741	arg1	chitosan					1747:1754	3% chitosan	1744:1754	3% chitosan	1744:1754	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	68	from	shark	1737:1741	arg1	%					1762:1762	10%	1760:1762	10% of fucoidan	1760:1774	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	69	theme	%	743:743	arg1	collagen					745:752	5% collagen	742:752	5% collagen from jellyfish	742:767	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	9	70	theme	structural	2466:2475	arg1	architecture					2477:2488	a good structural architecture	2459:2488	a good structural architecture	2459:2488	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	4	71	theme	shark/jellyfish	1069:1083	arg1	collagen					1085:1092	the shark/jellyfish collagen	1065:1092	the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively	1065:1202	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	71	theme	shark/jellyfish	1069:1083	arg1	s					1094:1094	s	1094:1094	s	1094:1094	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	7	72	theme	cellular	1959:1966	arg1	assessments					1968:1978	the in vitro cellular assessments	1946:1978	the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state	1946:2032	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	3	73	theme	%	777:777	arg1	collagen					779:786	3% collagen	776:786	3% collagen from blue shark skin	776:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	7	74	theme	in	1950:1951	arg1	assessments					1968:1978	the in vitro cellular assessments	1946:1978	the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state	1946:2032	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	1	75	theme	building	214:221	arg1	products					174:181	marine origin products	160:181	marine origin products	160:181	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	1	75	theme	building	214:221	arg1	blocks					223:228	building blocks	214:228	building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM)	214:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	6	76	theme	H3	1651:1652	arg1	formulation					1636:1646	the formulation	1632:1646	the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan)	1632:1775	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	77	from	solutions	729:737	arg1	skin					804:807	blue shark skin	793:807	blue shark skin	793:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	77	from	solutions	729:737	arg1	jellyfish					759:767	jellyfish	759:767	jellyfish	759:767	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	78	theme	shark	798:802	arg1	skin					804:807	blue shark skin	793:807	blue shark skin	793:807	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	8	79	dep	assay	2124:2128	arg1	assess					2163:2168	assess	2163:2168	to assess the cytoskeletal organization	2160:2198	Furthermore, Live/Dead assay and Phalloidin/DAPI staining, to assess the cytoskeletal organization, proved that the hydrogels can provide a suitable microenvironment for cell adhesion, viability, and proliferation, after being encapsulated.
35929272	4	80	dep	collagen	1085:1092	arg1	conserved					1097:1105	conserved	1097:1105	conserved the triple helical structure	1097:1134	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	80	dep	collagen	1085:1092	arg1	had					1140:1142	had	1140:1142	had	1140:1142	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	6	81	theme	viscoelastic	1530:1541	arg1	character					1543:1551	strong viscoelastic character	1523:1551	strong viscoelastic character	1523:1551	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	4	82	theme	helical	1118:1124	arg1	structure					1126:1134	the triple helical structure	1107:1134	the triple helical structure	1107:1134	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	5	83	theme	denaturation	1242:1253	arg1	temperature					1255:1265	a denaturation temperature	1240:1265	a denaturation temperature of around 30-32 °C and a molecular weight between 120 and 125 kDa	1240:1331	The studied collagens also contain a denaturation temperature of around 30-32 °C and a molecular weight between 120 and 125 kDa.
35929272	6	84	theme	hydrogel	1352:1359	arg1	properties					1361:1370	the hydrogel properties	1348:1370	the hydrogel properties	1348:1370	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	85	theme	squid	845:849	arg1	pens					851:854	squid pens	845:854	squid pens	845:854	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	8	86	theme	cell	2271:2274	arg1	adhesion					2276:2283	cell adhesion	2271:2283	cell adhesion	2271:2283	Furthermore, Live/Dead assay and Phalloidin/DAPI staining, to assess the cytoskeletal organization, proved that the hydrogels can provide a suitable microenvironment for cell adhesion, viability, and proliferation, after being encapsulated.
35929272	3	87	from	algae	897:901	arg1	pens					851:854	squid pens	845:854	squid pens	845:854	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	87	from	algae	897:901	arg1	solutions					860:868	solutions	860:868	solutions of 10% fucoidan from brown algae	860:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	6	88	theme	interesting	1499:1509	arg1	properties					1579:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	1	89	theme	marine	160:165	arg1	products					174:181	marine origin products	160:181	marine origin products	160:181	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	1	89	theme	marine	160:165	arg1	blocks					223:228	building blocks	214:228	building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM)	214:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	3	90	theme	%	875:875	arg1	fucoidan					877:884	10% fucoidan	873:884	10% fucoidan	873:884	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	4	91	theme	Amino	984:988	arg1	ATR-FTIR					1005:1012	ATR-FTIR	1005:1012	ATR-FTIR	1005:1012	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	91	theme	Amino	984:988	arg1	ICP					1029:1031	ICP	1029:1031	ICP	1029:1031	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	91	theme	Amino	984:988	arg1	XRD					1034:1036	XRD	1034:1036	XRD	1034:1036	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	91	theme	Amino	984:988	arg1	CD					1015:1016	CD	1015:1016	CD	1015:1016	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	91	theme	Amino	984:988	arg1	SDS-PAGE					1019:1026	SDS-PAGE	1019:1026	SDS-PAGE	1019:1026	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	4	91	theme	Amino	984:988	arg1	analysis					995:1002	Amino Acid analysis	984:1002	Amino Acid analysis	984:1002	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	6	92	theme	5	1687:1687	arg1	%					1688:1688	%	1688:1688	%	1688:1688	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	3	93	theme	chitosan	831:838	arg1	solutions					815:823	solutions	815:823	solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae	815:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	0	94	theme	cartilage	108:116	arg1	engineering					125:135	cartilage tissue engineering	108:135	cartilage tissue engineering	108:135	Marine origin biomaterials using a compressive and absorption methodology as cell-laden hydrogel envisaging cartilage tissue engineering.
35929272	1	95	theme	Regenerative	358:369	arg1	TERM					381:384	TERM	381:384	TERM	381:384	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	1	95	theme	Regenerative	358:369	arg1	Medicine					371:378	Regenerative Medicine	358:378	Regenerative Medicine (TERM)	358:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	6	96	dep	collagen	1690:1697	arg1	i.e.					1681:1684	i.e.	1681:1684	i.e.	1681:1684	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	2	97	theme	water	670:674	arg1	excess					660:665	the excess	656:665	the excess of water	656:674	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	3	98	theme	3	828:828	arg1	%					829:829	%	829:829	%	829:829	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	2	99	theme	absorption	534:543	arg1	methodology					545:555	a newly developed eco-friendly compressive and absorption methodology	487:555	methodology	545:555	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	7	100	theme	non-cytotoxic	2077:2089	arg1	behavior					2091:2098	a non-cytotoxic behavior	2075:2098	a non-cytotoxic behavior	2075:2098	Additionally, the in vitro cellular assessments using chondrocyte cells (ATDC5) in encapsulated state revealed, for all hydrogel formulations, a non-cytotoxic behavior.
35929272	0	101	theme	origin	7:12	arg1	biomaterials					14:25	Marine origin biomaterials	0:25	Marine origin biomaterials	0:25	Marine origin biomaterials using a compressive and absorption methodology as cell-laden hydrogel envisaging cartilage tissue engineering.
35929272	2	102	theme	hydrogel	460:467	arg1	combinations					410:421	several combinations	402:421	several combinations of marine collagen-chitosan-fucoidan hydrogel	402:467	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	6	103	theme	skin	1732:1735	arg1	shark					1737:1741	skin shark	1732:1741	skin shark	1732:1741	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	6	104	theme	structural	1558:1567	arg1	properties					1579:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	interesting mechanical (strong viscoelastic character) and structural stability properties	1499:1588	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	2	105	theme	marine	426:431	arg1	hydrogel					460:467	marine collagen-chitosan-fucoidan hydrogel	426:467	marine collagen-chitosan-fucoidan hydrogel	426:467	In this work, several combinations of marine collagen-chitosan-fucoidan hydrogel were formed using a newly developed eco-friendly compressive and absorption methodology to produce hydrogels (CAMPH), which consists of compacting the biopolymers solution while removing the excess of water.
35929272	0	106	theme	compressive	35:45	arg1	methodology					62:72	a compressive and absorption methodology	33:72	methodology	62:72	Marine origin biomaterials using a compressive and absorption methodology as cell-laden hydrogel envisaging cartilage tissue engineering.
35929272	9	107	theme	biomedical	2621:2630	arg1	therapies					2652:2660	biomedical articular cartilage therapies	2621:2660	biomedical articular cartilage therapies	2621:2660	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	6	108	theme	3	1715:1715	arg1	%					1716:1716	%	1716:1716	%	1716:1716	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	4	109	theme	biopolymer	929:938	arg1	characterization					957:972	The biopolymer physico-chemical characterization	925:972	The biopolymer physico-chemical characterization	925:972	The biopolymer physico-chemical characterization comprised Amino Acid analysis, ATR-FTIR, CD, SDS-PAGE, ICP, XRD, and the results suggested the shark/jellyfish collagen(s) conserved the triple helical structure and had similarities with type I and type II collagen, respectively.
35929272	0	110	theme	absorption	51:60	arg1	methodology					62:72	a compressive and absorption methodology	33:72	methodology	62:72	Marine origin biomaterials using a compressive and absorption methodology as cell-laden hydrogel envisaging cartilage tissue engineering.
35929272	1	111	theme	new	319:321	arg1	devices					323:329	new devices	319:329	new devices for Tissue Engineering and Regenerative Medicine (TERM)	319:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35929272	9	112	theme	cartilage	2642:2650	arg1	therapies					2652:2660	biomedical articular cartilage therapies	2621:2660	biomedical articular cartilage therapies	2621:2660	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	6	113	theme	mass	1885:1888	arg1	loss					1890:1893	a mass loss	1883:1893	a mass loss of around 35% and 44%, respectively	1883:1929	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	9	114	theme	marine	2377:2382	arg1	formulations					2438:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations	2373:2449	Overall, the results show that all marine collagen (jellyfish/shark)-chitosan-fucoidan hydrogel formulations provide a good structural architecture and microenvironment, highlighting the H3 biomaterial due to containing more polymers in their composition, making it suitable for biomedical articular cartilage therapies.
35929272	3	115	from	solutions	815:823	arg1	pens					851:854	squid pens	845:854	squid pens	845:854	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	115	from	solutions	815:823	arg1	solutions					860:868	solutions	860:868	solutions of 10% fucoidan from brown algae	860:901	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	3	116	theme	hydrogel	681:688	arg1	formulations					690:701	The hydrogel formulations	677:701	The hydrogel formulations	677:701	The hydrogel formulations were prepared by blending solutions of 5% collagen from jellyfish and/or 3% collagen from blue shark skin, with solutions of 3% chitosan from squid pens and solutions of 10% fucoidan from brown algae, at different ratios.
35929272	6	117	theme	uptake	1407:1412	arg1	ability					1414:1420	water uptake ability	1401:1420	water uptake ability	1401:1420	Additionally, the hydrogel properties were determined by rheology, water uptake ability, degradation rate, and SEM, and the results showed that all formulations had interesting mechanical (strong viscoelastic character) and structural stability properties, with a significant positive highlight in the formulation of H3 (blending all biopolymers, i.e., 5% collagen from jellyfish, 3% collagen from skin shark, 3% chitosan and 10% of fucoidan) in the degradation test, that shows a mass loss around 18% over the 30 days, while the H1 and H2, present a mass loss of around 35% and 44%, respectively.
35929272	1	118	theme	constant	249:256	arg1	demand					258:263	the constant demand	245:263	the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM)	245:385	In the recent decade, marine origin products have been growingly studied as building blocks complying with the constant demand of the biomedical sector regarding the development of new devices for Tissue Engineering and Regenerative Medicine (TERM).
35635563	7	0	theme	significant	1143:1153	arg1	effects					1155:1161	significant effects	1143:1161	significant effects	1143:1161	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	0	1	from	Toxicity	21:28	arg1	Sites					97:101	Two Contaminated Sites	80:101	Two Contaminated Sites	80:101	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	4	2	from	sites	751:755	arg1	%					776:776	52.02%	771:776	52.02%	771:776	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	4	2	from	sites	751:755	arg1	bioaccessibility					723:738	The average bioaccessibility	711:738	The average bioaccessibility of PAHs at sites S1 and S2	711:765	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	5	3	theme	compounds	840:848	arg1	bioaccessibility					808:823	The bioaccessibility	804:823	The bioaccessibility of certain PAH compounds	804:848	The bioaccessibility of certain PAH compounds decreased with increasing ring number of the molecule.
35635563	0	4	theme	Contaminated	84:95	arg1	Sites					97:101	Two Contaminated Sites	80:101	Two Contaminated Sites	80:101	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	7	5	theme	organic	1049:1055	arg1	matter					1057:1062	the dissolved organic matter	1035:1062	the dissolved organic matter	1035:1062	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	7	6	theme	total	1087:1091	arg1	potassium					1093:1101	total potassium	1087:1101	total potassium	1087:1101	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	0	7	from	Bioaccessibility	0:15	arg1	Sites					97:101	Two Contaminated Sites	80:101	Two Contaminated Sites	80:101	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	7	8	theme	dissolved	1039:1047	arg1	matter					1057:1062	the dissolved organic matter	1035:1062	the dissolved organic matter	1035:1062	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	3	9	theme	site	541:544	arg1	0.2-138.18 mg kg-1					584:601	0.2-138.18 mg kg-1	584:601	0.2-138.18 mg kg-1	584:601	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	3	9	theme	site	541:544	arg1	0.38-3342.5 mg kg-1					550:568	0.38-3342.5 mg kg-1	550:568	0.38-3342.5 mg kg-1	550:568	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	3	9	theme	site	541:544	arg1	S1					546:547	site S1	541:547	site S1 (0.38-3342.5 mg kg-1)	541:569	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	5	10	theme	PAH	836:838	arg1	compounds					840:848	certain PAH compounds	828:848	certain PAH compounds	828:848	The bioaccessibility of certain PAH compounds decreased with increasing ring number of the molecule.
35635563	6	11	from	textures	969:976	arg1	soil					992:995	sandy soil	986:995	sandy soil	986:995	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	9	12	theme	PAHs	1421:1424	arg1	toxicity					1409:1416	the toxicity	1405:1416	the toxicity of PAHs in soil	1405:1432	We suggest that bioaccessibility should be taken into consideration when assessing the toxicity of PAHs in soil, and more attention should be given to low-ring PAHs with high bioaccessibility.
35635563	7	13	theme	total	1108:1112	arg1	concentrations					1124:1137	total manganese concentrations	1108:1137	total manganese concentrations	1108:1137	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	3	14	theme	PAH	515:517	arg1	contamination					519:531	heavy PAH contamination	509:531	heavy PAH contamination	509:531	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	2	15	theme	hydroxypropyl-β-cyclodextrin	424:451	arg1	method					482:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	2	16	theme	residual	290:297	arg1	concentrations					299:312	the residual concentrations	286:312	the residual concentrations of PAHs in the soils of two industrial sites	286:357	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	7	17	theme	manganese	1114:1122	arg1	concentrations					1124:1137	total manganese concentrations	1108:1137	total manganese concentrations	1108:1137	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	4	18	theme	PAHs	743:746	arg1	%					776:776	52.02%	771:776	52.02%	771:776	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	4	18	theme	PAHs	743:746	arg1	bioaccessibility					723:738	The average bioaccessibility	711:738	The average bioaccessibility of PAHs at sites S1 and S2	711:765	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	2	19	theme	extraction	471:480	arg1	method					482:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	7	20	theme	soil	1018:1021	arg1	properties					1023:1032	the soil properties	1014:1032	the soil properties	1014:1032	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	9	21	theme	high	1492:1495	arg1	bioaccessibility					1497:1512	high bioaccessibility	1492:1512	high bioaccessibility	1492:1512	We suggest that bioaccessibility should be taken into consideration when assessing the toxicity of PAHs in soil, and more attention should be given to low-ring PAHs with high bioaccessibility.
35635563	5	22	theme	molecule	895:902	arg1	number					881:886	ring number	876:886	ring number of the molecule	876:902	The bioaccessibility of certain PAH compounds decreased with increasing ring number of the molecule.
35635563	3	23	theme	6-ring	660:665	arg1	compounds					667:675	6-ring compounds	660:675	6-ring compounds	660:675	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	6	24	from	loamy	948:952	arg1	soil					992:995	sandy soil	986:995	sandy soil	986:995	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	1	25	theme	Polycyclic	104:113	arg1	contaminants					164:175	ubiquitous soil contaminants	148:175	ubiquitous soil contaminants	148:175	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	1	25	theme	Polycyclic	104:113	arg1	PAHs					138:141	PAHs	138:141	PAHs	138:141	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	1	25	theme	Polycyclic	104:113	arg1	hydrocarbons					124:135	Polycyclic aromatic hydrocarbons	104:135	Polycyclic aromatic hydrocarbons (PAHs)	104:142	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	4	26	dep	sites	751:755	arg1	sites					751:755	sites S1 and S2	751:765	sites S1 and S2	751:765	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	4	26	dep	sites	751:755	arg1	S2					764:765	S2	764:765	S2	764:765	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	4	26	dep	sites	751:755	arg1	S1					757:758	S1	757:758	S1	757:758	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	2	27	theme	HPCD	465:468	arg1	method					482:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	3	28	theme	high	614:617	arg1	HMW					637:639	HMW	637:639	HMW	637:639	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	3	28	theme	high	614:617	arg1	weight					629:634	high molecular weight	614:634	high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds)	614:676	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	1	29	theme	aromatic	115:122	arg1	contaminants					164:175	ubiquitous soil contaminants	148:175	ubiquitous soil contaminants	148:175	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	1	29	theme	aromatic	115:122	arg1	PAHs					138:141	PAHs	138:141	PAHs	138:141	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	1	29	theme	aromatic	115:122	arg1	hydrocarbons					124:135	Polycyclic aromatic hydrocarbons	104:135	Polycyclic aromatic hydrocarbons (PAHs)	104:142	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	2	30	theme	extraction	453:462	arg1	method					482:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method	420:487	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	9	31	from	toxicity	1409:1416	arg1	soil					1429:1432	soil	1429:1432	soil	1429:1432	We suggest that bioaccessibility should be taken into consideration when assessing the toxicity of PAHs in soil, and more attention should be given to low-ring PAHs with high bioaccessibility.
35635563	6	32	theme	PAH	911:913	arg1	bioaccessibility					915:930	Lower PAH bioaccessibility	905:930	Lower PAH bioaccessibility	905:930	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	6	33	located	detected	936:943	arg1	loamy					948:952	loamy	948:952	loamy	948:952	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	6	33	located	detected	936:943	arg2	bioaccessibility					915:930	Lower PAH bioaccessibility	905:930	Lower PAH bioaccessibility	905:930	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	6	33	located	detected	936:943	arg1	textures					969:976	silty soil textures	958:976	silty soil textures	958:976	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	2	34	theme	PAHs	317:320	arg1	concentrations					299:312	the residual concentrations	286:312	the residual concentrations of PAHs in the soils of two industrial sites	286:357	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	1	35	theme	environmental	222:234	arg1	risks					236:240	their environmental risks	216:240	their environmental risks	216:240	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	0	36	theme	Polycyclic	44:53	arg1	Hydrocarbons					64:75	Polycyclic Aromatic Hydrocarbons	44:75	Polycyclic Aromatic Hydrocarbons	44:75	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	6	37	theme	Lower	905:909	arg1	bioaccessibility					915:930	Lower PAH bioaccessibility	905:930	Lower PAH bioaccessibility	905:930	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	7	38	theme	PAHs	1190:1193	arg1	bioaccessibility					1170:1185	the bioaccessibility	1166:1185	the bioaccessibility of PAHs	1166:1193	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	6	39	theme	sandy	986:990	arg1	soil					992:995	sandy soil	986:995	sandy soil	986:995	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	6	40	theme	soil	964:967	arg1	textures					969:976	silty soil textures	958:976	silty soil textures	958:976	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
35635563	4	41	theme	average	715:721	arg1	%					776:776	52.02%	771:776	52.02%	771:776	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	4	41	theme	average	715:721	arg1	bioaccessibility					723:738	The average bioaccessibility	711:738	The average bioaccessibility of PAHs at sites S1 and S2	711:765	The average bioaccessibility of PAHs at sites S1 and S2 was 52.02% and 29.28%, respectively.
35635563	0	42	theme	Hydrocarbons	64:75	arg1	Toxicity					21:28	Toxicity	21:28	Toxicity	21:28	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	0	42	theme	Hydrocarbons	64:75	arg1	Bioaccessibility					0:15	Bioaccessibility	0:15	Bioaccessibility	0:15	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	5	43	theme	certain	828:834	arg1	compounds					840:848	certain PAH compounds	828:848	certain PAH compounds	828:848	The bioaccessibility of certain PAH compounds decreased with increasing ring number of the molecule.
35635563	2	44	theme	sites	353:357	arg1	soils					329:333	the soils	325:333	the soils of two industrial sites	325:357	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	0	45	theme	Aromatic	55:62	arg1	Hydrocarbons					64:75	Polycyclic Aromatic Hydrocarbons	44:75	Polycyclic Aromatic Hydrocarbons	44:75	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	8	46	theme	PAHs	1270:1273	arg1	composition					1234:1244	composition	1234:1244	composition	1234:1244	The toxicity analysis showed that the composition and bioaccessibility of PAHs could affect their potential toxicity in soil.
35635563	8	46	theme	PAHs	1270:1273	arg1	bioaccessibility					1250:1265	bioaccessibility	1250:1265	bioaccessibility	1250:1265	The toxicity analysis showed that the composition and bioaccessibility of PAHs could affect their potential toxicity in soil.
35635563	7	47	contain	had	1139:1141	arg1	concentrations					1124:1137	total manganese concentrations	1108:1137	total manganese concentrations	1108:1137	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	7	47	contain	had	1139:1141	arg1	potassium					1093:1101	total potassium	1087:1101	total potassium	1087:1101	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	7	47	contain	had	1139:1141	arg2	effects					1155:1161	significant effects	1143:1161	significant effects	1143:1161	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	7	47	contain	had	1139:1141	arg1	carbon					1079:1084	total organic carbon	1065:1084	total organic carbon	1065:1084	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	7	47	contain	had	1139:1141	arg1	matter					1057:1062	the dissolved organic matter	1035:1062	the dissolved organic matter	1035:1062	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	2	48	theme	industrial	342:351	arg1	sites					353:357	two industrial sites	338:357	two industrial sites	338:357	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	8	49	from	toxicity	1304:1311	arg1	soil					1316:1319	soil	1316:1319	soil	1316:1319	The toxicity analysis showed that the composition and bioaccessibility of PAHs could affect their potential toxicity in soil.
35635563	3	50	theme	molecular	619:627	arg1	HMW					637:639	HMW	637:639	HMW	637:639	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	3	50	theme	molecular	619:627	arg1	weight					629:634	high molecular weight	614:634	high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds)	614:676	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	7	51	theme	organic	1071:1077	arg1	carbon					1079:1084	total organic carbon	1065:1084	total organic carbon	1065:1084	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	3	52	theme	weight	629:634	arg1	PAHs					642:645	high molecular weight (HMW) PAHs	614:645	high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds)	614:676	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	5	53	theme	ring	876:879	arg1	number					881:886	ring number	876:886	ring number of the molecule	876:902	The bioaccessibility of certain PAH compounds decreased with increasing ring number of the molecule.
35635563	3	54	theme	site	575:578	arg1	S2					580:581	site S2	575:581	site S2	575:581	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	1	55	theme	contaminated	245:256	arg1	land					258:261	contaminated land	245:261	contaminated land	245:261	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	8	56	theme	potential	1294:1302	arg1	toxicity					1304:1311	their potential toxicity	1288:1311	their potential toxicity in soil	1288:1319	The toxicity analysis showed that the composition and bioaccessibility of PAHs could affect their potential toxicity in soil.
35635563	9	57	theme	low-ring	1473:1480	arg1	PAHs					1482:1485	low-ring PAHs	1473:1485	low-ring PAHs with high bioaccessibility	1473:1512	We suggest that bioaccessibility should be taken into consideration when assessing the toxicity of PAHs in soil, and more attention should be given to low-ring PAHs with high bioaccessibility.
35635563	8	58	dep	composition	1234:1244	arg1	the					1230:1232	the	1230:1232	the	1230:1232	The toxicity analysis showed that the composition and bioaccessibility of PAHs could affect their potential toxicity in soil.
35635563	3	59	theme	heavy	509:513	arg1	contamination					519:531	heavy PAH contamination	509:531	heavy PAH contamination	509:531	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	9	60	theme	more	1439:1442	arg1	attention					1444:1452	more attention	1439:1452	more attention	1439:1452	We suggest that bioaccessibility should be taken into consideration when assessing the toxicity of PAHs in soil, and more attention should be given to low-ring PAHs with high bioaccessibility.
35635563	9	61	with	PAHs	1482:1485	arg1	bioaccessibility					1497:1512	high bioaccessibility	1492:1512	high bioaccessibility	1492:1512	We suggest that bioaccessibility should be taken into consideration when assessing the toxicity of PAHs in soil, and more attention should be given to low-ring PAHs with high bioaccessibility.
35635563	2	62	theme	present	271:277	arg1	study					279:283	the present study	267:283	the present study	267:283	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	8	63	theme	toxicity	1200:1207	arg1	analysis					1209:1216	The toxicity analysis	1196:1216	The toxicity analysis	1196:1216	The toxicity analysis showed that the composition and bioaccessibility of PAHs could affect their potential toxicity in soil.
35635563	1	64	theme	ubiquitous	148:157	arg1	contaminants					164:175	ubiquitous soil contaminants	148:175	ubiquitous soil contaminants	148:175	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	1	64	theme	ubiquitous	148:157	arg1	hydrocarbons					124:135	Polycyclic aromatic hydrocarbons	104:135	Polycyclic aromatic hydrocarbons (PAHs)	104:142	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	2	65	from	concentrations	299:312	arg1	soils					329:333	the soils	325:333	the soils of two industrial sites	325:357	In the present study, the residual concentrations of PAHs in the soils of two industrial sites were determined, and their bioaccessibility was estimated by the hydroxypropyl-β-cyclodextrin extraction (HPCD) extraction method.
35635563	3	66	dep	PAHs	642:645	arg1	compounds					667:675	6-ring compounds	660:675	6-ring compounds	660:675	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	3	66	dep	PAHs	642:645	arg1	5-					652:653	5-	652:653	5-	652:653	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	3	66	dep	PAHs	642:645	arg1	4-					648:649	4-	648:649	4-	648:649	The results showed heavy PAH contamination at both site S1 (0.38-3342.5 mg kg-1) and site S2 (0.2-138.18 mg kg-1), of which high molecular weight (HMW) PAHs (4-, 5-, and 6-ring compounds) accounted for approximately 80%.
35635563	7	67	theme	total	1065:1069	arg1	carbon					1079:1084	total organic carbon	1065:1084	total organic carbon	1065:1084	Moreover, among the soil properties, the dissolved organic matter, total organic carbon, total potassium, and total manganese concentrations had significant effects on the bioaccessibility of PAHs.
35635563	0	68	dep	Bioaccessibility	0:15	arg1	Assessment					30:39	Assessment	30:39	Assessment	30:39	Bioaccessibility and Toxicity Assessment of Polycyclic Aromatic Hydrocarbons in Two Contaminated Sites.
35635563	1	69	theme	soil	159:162	arg1	contaminants					164:175	ubiquitous soil contaminants	148:175	ubiquitous soil contaminants	148:175	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	1	69	theme	soil	159:162	arg1	hydrocarbons					124:135	Polycyclic aromatic hydrocarbons	104:135	Polycyclic aromatic hydrocarbons (PAHs)	104:142	Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous soil contaminants, and their bioaccessibility determines their environmental risks in contaminated land.
35635563	6	70	theme	silty	958:962	arg1	textures					969:976	silty soil textures	958:976	silty soil textures	958:976	Lower PAH bioaccessibility was detected in loamy and silty soil textures than in sandy soil.
36707764	4	0	theme	community	800:808	arg1	composition					810:820	community composition	800:820	community composition	800:820	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	3	1	theme	MRE	547:549	arg1	intervention					551:562	MRE intervention	547:562	MRE intervention	547:562	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	2	2	theme	sudden	309:314	arg1	change					316:321	a sudden change	307:321	a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration,	307:415	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	5	3	from	taxa	940:943	arg1	changes					905:911	changes	905:911	changes in relative abundance from taxa, genes and pathways	905:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	5	3	from	taxa	940:943	arg1	abundance					925:933	relative abundance	916:933	relative abundance from taxa, genes and pathways	916:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	7	4	from	changes	1501:1507	arg1	structure					1527:1535	gut microbiota structure	1512:1535	gut microbiota structure	1512:1535	CONCLUSIONS These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity.
36707764	6	5	theme	Enzymes	1263:1269	arg1	abundance					1007:1015	relative abundance	998:1015	relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation	998:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	2	6	dep	in	250:251	arg1	vitro					253:257	vitro	253:257	vitro	253:257	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	8	7	theme	candidate	1685:1693	arg1	strategy					1708:1715	a candidate supplemental strategy	1683:1715	a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states	1683:1793	Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.
36707764	7	8	theme	microbiota	1516:1525	arg1	structure					1527:1535	gut microbiota structure	1512:1535	gut microbiota structure	1512:1535	CONCLUSIONS These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity.
36707764	0	9	theme	sudden	90:95	arg1	change					97:102	a sudden change	88:102	a sudden change in diet	88:110	In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.
36707764	6	10	theme	Carbohydrate-Active	1243:1261	arg1	Enzymes					1263:1269	nine Carbohydrate-Active Enzymes	1238:1269	nine Carbohydrate-Active Enzymes	1238:1269	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	6	10	theme	Carbohydrate-Active	1243:1261	arg1	GH13_14					1285:1291	GH13_14	1285:1291	specifically GH13_14	1272:1291	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	5	11	theme	relative	916:923	arg1	abundance					925:933	relative abundance	916:933	relative abundance from taxa, genes and pathways	916:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	2	12	theme	functional	450:459	arg1	potential					461:469	functional potential	450:469	functional potential of the human gut microbiota	450:497	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	6	13	dep	RESULTS	966:972	arg1	changes					987:993	Differential changes	974:993	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation	966:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	2	14	theme	in	250:251	arg1	fermentation					259:270	in vitro fermentation	250:270	in vitro fermentation	250:270	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	5	15	from	genes	946:950	arg1	changes					905:911	changes	905:911	changes in relative abundance from taxa, genes and pathways	905:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	5	15	from	genes	946:950	arg1	abundance					925:933	relative abundance	916:933	relative abundance from taxa, genes and pathways	916:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	2	16	from	effects	296:302	arg1	sustenance					345:354	21 days sole sustenance	332:354	21 days sole sustenance on the Meal	332:366	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	16	from	effects	296:302	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	16	from	effects	296:302	arg1	ration					409:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	16	from	effects	296:302	arg1	competition					434:444	inter-species competition	420:444	inter-species competition	420:444	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	16	from	effects	296:302	arg1	potential					461:469	functional potential	450:469	functional potential of the human gut microbiota	450:497	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	5	17	from	pathways	956:963	arg1	changes					905:911	changes	905:911	changes in relative abundance from taxa, genes and pathways	905:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	5	17	from	pathways	956:963	arg1	abundance					925:933	relative abundance	916:933	relative abundance from taxa, genes and pathways	916:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	2	18	theme	sole	340:343	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	18	theme	sole	340:343	arg1	sustenance					345:354	21 days sole sustenance	332:354	21 days sole sustenance on the Meal	332:366	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	7	19	theme	metabolic	1595:1603	arg1	capacity					1605:1612	metabolic capacity	1595:1612	metabolic capacity	1595:1612	CONCLUSIONS These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity.
36707764	6	20	theme	specific	1392:1399	arg1	taxa					1401:1404	specific taxa	1392:1404	specific taxa of interest	1392:1416	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	6	21	theme	interest	1409:1416	arg1	taxa					1401:1404	specific taxa	1392:1404	specific taxa of interest	1392:1416	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	2	22	theme	21 days	332:338	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	22	theme	21 days	332:338	arg1	sustenance					345:354	21 days sole sustenance	332:354	21 days sole sustenance on the Meal	332:366	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	23	theme	MRE	383:385	arg1	ration					409:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	23	theme	MRE	383:385	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	6	24	theme	intervention	1361:1372	arg1	RESULTS					966:972	RESULTS	966:972	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation	966:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	6	24	theme	intervention	1361:1372	arg1	function					1340:1347	a function	1338:1347	a function of the diet intervention	1338:1372	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	3	25	theme	Human	500:504	arg1	samples					512:518	Human fecal samples	500:518	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB)	500:597	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	4	26	theme	functional	826:835	arg1	potential					837:845	functional potential	826:845	functional potential	826:845	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	1	27	theme	gut	166:168	arg1	microbiome					170:179	the gut microbiome	162:179	the gut microbiome	162:179	BACKGROUND Interactions between diet, stress and the gut microbiome are of interest as a means to modulate health and performance.
36707764	0	28	theme	In	0:1	arg1	response					24:31	In vitro gut microbiome response	0:31	In vitro gut microbiome response to carbohydrate supplementation	0:63	In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.
36707764	3	29	theme	fecal	506:510	arg1	samples					512:518	Human fecal samples	500:518	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB)	500:597	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	8	30	theme	specific	1742:1749	arg1	commensals					1755:1764	specific gut commensals	1742:1764	specific gut commensals	1742:1764	Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.
36707764	0	31	theme	microbiome	13:22	arg1	response					24:31	In vitro gut microbiome response	0:31	In vitro gut microbiome response to carbohydrate supplementation	0:63	In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.
36707764	2	32	theme	gut	484:486	arg1	microbiota					488:497	the human gut microbiota	474:497	the human gut microbiota	474:497	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	33	from	change	316:321	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	33	from	change	316:321	arg1	ration					409:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	33	from	change	316:321	arg1	sustenance					345:354	21 days sole sustenance	332:354	21 days sole sustenance on the Meal	332:366	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	0	34	theme	gut	9:11	arg1	response					24:31	In vitro gut microbiome response	0:31	In vitro gut microbiome response to carbohydrate supplementation	0:63	In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.
36707764	3	35	theme	in	667:668	arg1	fermentation					676:687	in vitro fermentation	667:687	in vitro fermentation	667:687	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	2	36	theme	human	478:482	arg1	microbiota					488:497	the human gut microbiota	474:497	the human gut microbiota	474:497	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	3	37	theme	habitual	579:586	arg1	HAB					594:596	HAB	594:596	HAB	594:596	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	3	37	theme	habitual	579:586	arg1	diet					588:591	consuming a habitual diet	567:591	consuming a habitual diet (HAB)	567:597	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	5	38	theme	Specific	848:855	arg1	analyses					869:876	Specific statistical analyses	848:876	Specific statistical analyses	848:876	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	8	39	theme	starch	1673:1678	arg1	potential					1660:1668	the potential	1656:1668	the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states	1656:1793	Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.
36707764	2	40	theme	combat	402:407	arg1	ration					409:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	40	theme	combat	402:407	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	6	41	dep	Bacteroides	1166:1176	arg1	egerthii					1178:1185	Bacteroides egerthii	1166:1185	Bacteroides egerthii	1166:1185	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	6	42	theme	24 h	1303:1306	arg1	fermentation					1308:1319	the 24 h fermentation	1299:1319	the 24 h fermentation	1299:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	8	43	theme	gut	1751:1753	arg1	commensals					1755:1764	specific gut commensals	1742:1764	specific gut commensals	1742:1764	Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.
36707764	6	44	theme	relative	998:1005	arg1	abundance					1007:1015	relative abundance	998:1015	relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation	998:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	2	45	theme	military	393:400	arg1	ration					409:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	45	theme	military	393:400	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	4	46	used	used	784:787	arg2	WMS					774:776	WMS	774:776	WMS	774:776	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	4	46	used	used	784:787	arg2	sequencing					762:771	Whole-metagenome sequencing	745:771	Whole-metagenome sequencing (WMS)	745:777	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	4	46	used	used	784:787	arg2	amplicon					732:739	16S rRNA amplicon	723:739	16S rRNA amplicon	723:739	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	3	47	theme	consuming	567:575	arg1	HAB					594:596	HAB	594:596	HAB	594:596	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	3	47	theme	consuming	567:575	arg1	diet					588:591	consuming a habitual diet	567:591	consuming a habitual diet (HAB)	567:597	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	2	48	theme	Ready-to-Eat	369:380	arg1	ration					409:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	48	theme	Ready-to-Eat	369:380	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	8	49	theme	stress-induced	1773:1786	arg1	states					1788:1793	stress-induced states	1773:1793	stress-induced states	1773:1793	Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.
36707764	6	50	theme	taxa	1023:1026	arg1	abundance					1007:1015	relative abundance	998:1015	relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation	998:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	2	51	theme	U.S.	388:391	arg1	ration					409:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Ready-to-Eat (MRE) U.S. military combat ration	369:414	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	51	theme	U.S.	388:391	arg1	diet					326:329	diet	326:329	diet	326:329	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	7	52	dep	CONCLUSIONS	1419:1429	arg1	suggest					1446:1452	suggest	1446:1452	suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity	1446:1612	CONCLUSIONS These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity.
36707764	7	53	theme	gut	1512:1514	arg1	structure					1527:1535	gut microbiota structure	1512:1535	gut microbiota structure	1512:1535	CONCLUSIONS These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity.
36707764	2	54	from	diet	326:329	arg1	effects					296:302	the effects	292:302	the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota	292:497	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	5	55	theme	statistical	857:867	arg1	analyses					869:876	Specific statistical analyses	848:876	Specific statistical analyses	848:876	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	0	56	theme	carbohydrate	36:47	arg1	supplementation					49:63	carbohydrate supplementation	36:63	carbohydrate supplementation	36:63	In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.
36707764	3	57	theme	ascending	695:703	arg1	conditions					711:720	ascending colon conditions	695:720	ascending colon conditions	695:720	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	3	58	dep	in	667:668	arg1	vitro					670:674	vitro	670:674	vitro	670:674	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	6	59	from	changes	987:993	arg1	abundance					1007:1015	relative abundance	998:1015	relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation	998:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	5	60	from	changes	905:911	arg1	abundance					925:933	relative abundance	916:933	relative abundance from taxa, genes and pathways	916:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	5	60	from	changes	905:911	arg1	pathways					956:963	pathways	956:963	pathways	956:963	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	5	60	from	changes	905:911	arg1	genes					946:950	genes	946:950	genes	946:950	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	5	60	from	changes	905:911	arg1	taxa					940:943	taxa	940:943	taxa	940:943	Specific statistical analyses were implemented to detect changes in relative abundance from taxa, genes and pathways.
36707764	3	61	theme	colon	705:709	arg1	conditions					711:720	ascending colon conditions	695:720	ascending colon conditions	695:720	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	0	62	from	change	97:102	arg1	diet					107:110	diet	107:110	diet	107:110	In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.
36707764	4	63	theme	rRNA	727:730	arg1	amplicon					732:739	16S rRNA amplicon	723:739	16S rRNA amplicon	723:739	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	4	64	theme	Whole-metagenome	745:760	arg1	sequencing					762:771	Whole-metagenome sequencing	745:771	Whole-metagenome sequencing (WMS)	745:777	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	4	64	theme	Whole-metagenome	745:760	arg1	WMS					774:776	WMS	774:776	WMS	774:776	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	1	65	theme	BACKGROUND	113:122	arg1	Interactions					124:135	BACKGROUND Interactions	113:135	BACKGROUND Interactions between diet, stress and the gut microbiome	113:179	BACKGROUND Interactions between diet, stress and the gut microbiome are of interest as a means to modulate health and performance.
36707764	8	66	theme	supplemental	1695:1706	arg1	strategy					1708:1715	a candidate supplemental strategy	1683:1715	a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states	1683:1793	Additionally, these findings demonstrate the potential of starch as a candidate supplemental strategy to functionally modulate specific gut commensals during stress-induced states.
36707764	2	67	theme	microbiota	488:497	arg1	competition					434:444	inter-species competition	420:444	inter-species competition	420:444	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	2	67	theme	microbiota	488:497	arg1	potential					461:469	functional potential	450:469	functional potential of the human gut microbiota	450:497	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	4	68	theme	16S	723:725	arg1	rRNA					727:730	16S rRNA	723:730	16S rRNA amplicon	723:739	16S rRNA amplicon and Whole-metagenome sequencing (WMS) were used to measure community composition and functional potential.
36707764	2	69	used	used	276:279	arg2	fermentation					259:270	in vitro fermentation	250:270	in vitro fermentation	250:270	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	6	70	theme	Differential	974:985	arg1	changes					987:993	Differential changes	974:993	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation	966:1319	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	2	71	theme	inter-species	420:432	arg1	competition					434:444	inter-species competition	420:444	inter-species competition	420:444	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	7	72	dep	carbohydrate	1552:1563	arg1	response					1540:1547	response	1540:1547	response	1540:1547	CONCLUSIONS These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity.
36707764	2	73	from	sustenance	345:354	arg1	Meal					363:366	the Meal	359:366	the Meal	359:366	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
36707764	7	74	from	alterations	1580:1590	arg1	capacity					1605:1612	metabolic capacity	1595:1612	metabolic capacity	1595:1612	CONCLUSIONS These findings suggest that consuming MRE for 21 days acutely effects changes in gut microbiota structure in response to carbohydrate but may induce alterations in metabolic capacity.
36707764	6	75	theme	diet	1356:1359	arg1	intervention					1361:1372	the diet intervention	1352:1372	the diet intervention	1352:1372	RESULTS Differential changes in relative abundance of 11 taxa, Dorea, Lachnospira, Bacteroides fragilis, Akkermansia muciniphila, Bifidobacterium adolescentis, Betaproteobacteria, Enterobacteriaceae, Bacteroides egerthii, Ruminococcus bromii, Prevotella, and Slackia, and nine Carbohydrate-Active Enzymes, specifically GH13_14, over the 24 h fermentation were observed as a function of the diet intervention and correlated to specific taxa of interest.
36707764	3	76	theme	nutrient-rich	618:630	arg1	media					632:636	nutrient-rich media	618:636	nutrient-rich media supplemented with starch for in vitro fermentation	618:687	Human fecal samples collected before and after MRE intervention or consuming a habitual diet (HAB) were introduced to nutrient-rich media supplemented with starch for in vitro fermentation under ascending colon conditions.
36707764	0	77	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro gut microbiome response to carbohydrate supplementation is acutely affected by a sudden change in diet.
36707764	2	78	theme	change	316:321	arg1	effects					296:302	the effects	292:302	the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota	292:497	Here, in vitro fermentation was used to explore the effects of a sudden change in diet, 21 days sole sustenance on the Meal, Ready-to-Eat (MRE) U.S. military combat ration, on inter-species competition and functional potential of the human gut microbiota.
35961550	2	0	theme	synthesis	485:493	arg1	procedure					495:503	The synthesis procedure	481:503	The synthesis procedure	481:503	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	5	1	theme	great	1150:1154	arg1	promise					1156:1162	great promise	1150:1162	great promise for skin biomedical applications	1150:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	2	2	theme	graphene	556:563	arg1	nanoplatelets					565:577	graphene nanoplatelets	556:577	graphene nanoplatelets (GNPs)	556:584	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	2	2	theme	graphene	556:563	arg1	GNPs					580:583	GNPs	580:583	GNPs	580:583	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	5	3	theme	hydrophilic	1105:1115	arg1	film					1139:1142	This new hydrophilic composite biomaterial film	1096:1142	This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications	1096:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	3	theme	hydrophilic	1105:1115	arg1	nanosheets					1084:1093	the GNP nanosheets	1076:1093	the GNP nanosheets	1076:1093	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	4	theme	antiviral	1228:1236	arg1	materials					1277:1285	antiviral and/or biodegradable electroconductive materials	1228:1285	antiviral and/or biodegradable electroconductive materials	1228:1285	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	0	5	theme	graphene	83:90	arg1	nanoplatelets					92:104	graphene nanoplatelets	83:104	graphene nanoplatelets	83:104	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	4	6	theme	components	866:875	arg1	absorption					816:825	water absorption	810:825	water absorption	810:825	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	4	6	theme	components	866:875	arg1	conductivity					841:852	electrical conductivity	830:852	electrical conductivity	830:852	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	1	7	theme	calcium	362:368	arg1	CA					380:381	CA	380:381	CA	380:381	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	7	theme	calcium	362:368	arg1	materials					302:310	two US Food and Drug Administration approved materials	257:310	two US Food and Drug Administration approved materials	257:310	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	7	theme	calcium	362:368	arg1	alginate					370:377	calcium alginate	362:377	calcium alginate (CA)	362:382	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	5	8	theme	chains	1040:1045	arg1	rearrangements					998:1011	molecular rearrangements	988:1011	molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials	988:1285	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	1	9	theme	Drug	273:276	arg1	Administration					278:291	Drug Administration	273:291	Drug Administration	273:291	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	3	10	theme	novel	723:727	arg1	semi-IPN					729:736	the novel semi-IPN	719:736	the novel semi-IPN against a surrogate virus of SARS-CoV-2	719:776	This quantity of multilayer graphene provides superior antiviral activity to the novel semi-IPN against a surrogate virus of SARS-CoV-2.
35961550	4	11	theme	CA	880:881	arg1	components					866:875	the pure components	857:875	the pure components of CA and PHBV	857:890	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	1	12	theme	poor	449:452	arg1	properties					463:472	the poor adhesion properties	445:472	the poor adhesion properties of CA	445:478	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	5	13	theme	electroconductive	1259:1275	arg1	materials					1277:1285	antiviral and/or biodegradable electroconductive materials	1228:1285	antiviral and/or biodegradable electroconductive materials	1228:1285	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	0	14	theme	antiviral	122:130	arg1	activity					132:139	antiviral activity	122:139	antiviral activity	122:139	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	5	15	theme	composite	1117:1125	arg1	film					1139:1142	This new hydrophilic composite biomaterial film	1096:1142	This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications	1096:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	15	theme	composite	1117:1125	arg1	nanosheets					1084:1093	the GNP nanosheets	1076:1093	the GNP nanosheets	1076:1093	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	2	16	theme	w/w	549:551	arg1	nanoplatelets					565:577	graphene nanoplatelets	556:577	graphene nanoplatelets (GNPs)	556:584	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	2	16	theme	w/w	549:551	arg1	%					547:547	10 % w/w	544:551	10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes	544:639	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	2	16	theme	w/w	549:551	arg1	GNPs					580:583	GNPs	580:583	GNPs	580:583	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	1	17	theme	adhesion	454:461	arg1	properties					463:472	the poor adhesion properties	445:472	the poor adhesion properties of CA	445:478	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	5	18	theme	biomaterial	1127:1137	arg1	film					1139:1142	This new hydrophilic composite biomaterial film	1096:1142	This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications	1096:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	18	theme	biomaterial	1127:1137	arg1	nanosheets					1084:1093	the GNP nanosheets	1076:1093	the GNP nanosheets	1076:1093	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	1	19	theme	new	188:190	arg1	network					235:241	A new biodegradable semi-interpenetrated polymer network	186:241	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA)	186:382	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	19	theme	new	188:190	arg1	semi-IPN					244:251	semi-IPN	244:251	semi-IPN	244:251	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	0	20	theme	alginate	12:19	arg1	films					30:34	alginate hydrogel films	12:34	alginate hydrogel films	12:34	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	2	21	theme	cytotoxic	601:609	arg1	effect					611:616	no cytotoxic effect	598:616	no cytotoxic effect	598:616	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	3	22	theme	surrogate	748:756	arg1	virus					758:762	a surrogate virus	746:762	a surrogate virus of SARS-CoV-2	746:776	This quantity of multilayer graphene provides superior antiviral activity to the novel semi-IPN against a surrogate virus of SARS-CoV-2.
35961550	1	23	theme	biodegradable	192:204	arg1	network					235:241	A new biodegradable semi-interpenetrated polymer network	186:241	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA)	186:382	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	23	theme	biodegradable	192:204	arg1	semi-IPN					244:251	semi-IPN	244:251	semi-IPN	244:251	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	2	24	theme	%	547:547	arg1	incorporation					527:539	the additional incorporation	512:539	the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes	512:639	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	1	25	theme	approved	293:300	arg1	poly					313:316	poly	313:316	poly(3-hydroxybutyrate-co-3-valerate) (PHBV)	313:356	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	25	theme	approved	293:300	arg1	materials					302:310	two US Food and Drug Administration approved materials	257:310	two US Food and Drug Administration approved materials	257:310	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	25	theme	approved	293:300	arg1	alginate					370:377	calcium alginate	362:377	calcium alginate (CA)	362:382	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	26	theme	CA	477:478	arg1	properties					463:472	the poor adhesion properties	445:472	the poor adhesion properties of CA	445:478	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	27	theme	semi-interpenetrated	206:225	arg1	network					235:241	A new biodegradable semi-interpenetrated polymer network	186:241	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA)	186:382	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	27	theme	semi-interpenetrated	206:225	arg1	semi-IPN					244:251	semi-IPN	244:251	semi-IPN	244:251	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	28	theme	materials	302:310	arg1	network					235:241	A new biodegradable semi-interpenetrated polymer network	186:241	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA)	186:382	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	28	theme	materials	302:310	arg1	semi-IPN					244:251	semi-IPN	244:251	semi-IPN	244:251	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	2	29	theme	human	621:625	arg1	keratinocytes					627:639	human keratinocytes	621:639	human keratinocytes	621:639	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	1	30	theme	polymer	227:233	arg1	network					235:241	A new biodegradable semi-interpenetrated polymer network	186:241	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA)	186:382	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	30	theme	polymer	227:233	arg1	semi-IPN					244:251	semi-IPN	244:251	semi-IPN	244:251	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	0	31	theme	hydrogel	21:28	arg1	films					30:34	alginate hydrogel films	12:34	alginate hydrogel films	12:34	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	0	32	theme	activity	132:139	arg1	Enhancement					107:117	Enhancement	107:117	Enhancement of antiviral activity, cell adhesion and electroactive properties	107:183	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	0	33	theme	adhesion	147:154	arg1	Enhancement					107:117	Enhancement	107:117	Enhancement of antiviral activity, cell adhesion and electroactive properties	107:183	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	1	34	dep	US	261:262	arg1	Food					264:267	Food	264:267	Food	264:267	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	1	34	dep	US	261:262	arg1	Administration					278:291	Drug Administration	273:291	Drug Administration	273:291	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	5	35	theme	intertwined	1020:1030	arg1	those					1209:1213	those	1209:1213	those	1209:1213	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	35	theme	intertwined	1020:1030	arg1	chains					1040:1045	the intertwined polymer chains	1016:1045	the intertwined polymer chains	1016:1045	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	4	36	dep	absorption	816:825	arg1	the					806:808	the	806:808	the	806:808	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	5	37	theme	skin	1168:1171	arg1	applications					1184:1195	skin biomedical applications	1168:1195	skin biomedical applications	1168:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	0	38	theme	cell	142:145	arg1	adhesion					147:154	cell adhesion	142:154	cell adhesion	142:154	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	4	39	theme	water	810:814	arg1	absorption					816:825	water absorption	810:825	water absorption	810:825	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	5	40	theme	biomedical	1173:1182	arg1	applications					1184:1195	skin biomedical applications	1168:1195	skin biomedical applications	1168:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	0	41	theme	electroactive	160:172	arg1	properties					174:183	electroactive properties	160:183	electroactive properties	160:183	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	5	42	theme	new	1101:1103	arg1	film					1139:1142	This new hydrophilic composite biomaterial film	1096:1142	This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications	1096:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	42	theme	new	1101:1103	arg1	nanosheets					1084:1093	the GNP nanosheets	1076:1093	the GNP nanosheets	1076:1093	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	4	43	theme	electrical	830:839	arg1	conductivity					841:852	electrical conductivity	830:852	electrical conductivity	830:852	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	3	44	theme	superior	688:695	arg1	activity					707:714	superior antiviral activity	688:714	superior antiviral activity	688:714	This quantity of multilayer graphene provides superior antiviral activity to the novel semi-IPN against a surrogate virus of SARS-CoV-2.
35961550	4	45	theme	PHBV	887:890	arg1	components					866:875	the pure components	857:875	the pure components of CA and PHBV	857:890	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	3	46	theme	multilayer	659:668	arg1	graphene					670:677	multilayer graphene	659:677	multilayer graphene	659:677	This quantity of multilayer graphene provides superior antiviral activity to the novel semi-IPN against a surrogate virus of SARS-CoV-2.
35961550	3	47	theme	graphene	670:677	arg1	quantity					647:654	This quantity	642:654	This quantity of multilayer graphene	642:677	This quantity of multilayer graphene provides superior antiviral activity to the novel semi-IPN against a surrogate virus of SARS-CoV-2.
35961550	3	48	theme	SARS-CoV-2	767:776	arg1	virus					758:762	a surrogate virus	746:762	a surrogate virus of SARS-CoV-2	746:776	This quantity of multilayer graphene provides superior antiviral activity to the novel semi-IPN against a surrogate virus of SARS-CoV-2.
35961550	2	49	theme	nanoplatelets	565:577	arg1	nanoplatelets					565:577	graphene nanoplatelets	556:577	graphene nanoplatelets (GNPs)	556:584	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	2	49	theme	nanoplatelets	565:577	arg1	%					547:547	10 % w/w	544:551	10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes	544:639	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	2	49	theme	nanoplatelets	565:577	arg1	GNPs					580:583	GNPs	580:583	GNPs	580:583	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	1	50	theme	alternative	413:423	arg1	strategy					425:432	an alternative strategy	410:432	an alternative strategy to enhance the poor adhesion properties of CA	410:478	A new biodegradable semi-interpenetrated polymer network (semi-IPN) of two US Food and Drug Administration approved materials, poly(3-hydroxybutyrate-co-3-valerate) (PHBV) and calcium alginate (CA) was engineered to provide an alternative strategy to enhance the poor adhesion properties of CA.
35961550	5	51	theme	molecular	988:996	arg1	rearrangements					998:1011	molecular rearrangements	988:1011	molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials	988:1285	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	52	theme	polymer	1032:1038	arg1	those					1209:1213	those	1209:1213	those	1209:1213	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	52	theme	polymer	1032:1038	arg1	chains					1040:1045	the intertwined polymer chains	1016:1045	the intertwined polymer chains	1016:1045	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	0	53	theme	properties	174:183	arg1	Enhancement					107:117	Enhancement	107:117	Enhancement of antiviral activity, cell adhesion and electroactive properties	107:183	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	4	54	theme	pure	861:864	arg1	components					866:875	the pure components	857:875	the pure components of CA and PHBV	857:890	Adding GNPs hardly affects the water absorption or electrical conductivity of the pure components of CA and PHBV.
35961550	5	55	theme	electrical	917:926	arg1	conductivity					928:939	the semi-IPN's electrical conductivity	902:939	the semi-IPN's electrical conductivity	902:939	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	2	56	theme	additional	516:525	arg1	incorporation					527:539	the additional incorporation	512:539	the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes	512:639	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	5	57	theme	GNP	1080:1082	arg1	film					1139:1142	This new hydrophilic composite biomaterial film	1096:1142	This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications	1096:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	5	57	theme	GNP	1080:1082	arg1	nanosheets					1084:1093	the GNP nanosheets	1076:1093	the GNP nanosheets	1076:1093	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	2	58	contain	have	593:596	arg1	nanoplatelets					565:577	graphene nanoplatelets	556:577	graphene nanoplatelets (GNPs)	556:584	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	2	58	contain	have	593:596	arg2	effect					611:616	no cytotoxic effect	598:616	no cytotoxic effect	598:616	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	2	58	contain	have	593:596	arg1	GNPs					580:583	GNPs	580:583	GNPs	580:583	The synthesis procedure allows the additional incorporation of 10 % w/w of graphene nanoplatelets (GNPs), which have no cytotoxic effect on human keratinocytes.
35961550	5	59	theme	biodegradable	1245:1257	arg1	materials					1277:1285	antiviral and/or biodegradable electroconductive materials	1228:1285	antiviral and/or biodegradable electroconductive materials	1228:1285	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
35961550	3	60	theme	antiviral	697:705	arg1	activity					707:714	superior antiviral activity	688:714	superior antiviral activity	688:714	This quantity of multilayer graphene provides superior antiviral activity to the novel semi-IPN against a surrogate virus of SARS-CoV-2.
35961550	0	61	dep	Engineering	0:10	arg1	Enhancement					107:117	Enhancement	107:117	Enhancement of antiviral activity, cell adhesion and electroactive properties	107:183	Engineering alginate hydrogel films with poly(3-hydroxybutyrate-co-3-valerate) and graphene nanoplatelets: Enhancement of antiviral activity, cell adhesion and electroactive properties.
35961550	5	62	dep	film	1139:1142	arg1	shows					1144:1148	shows	1144:1148	shows great promise for skin biomedical applications	1144:1195	However, the semi-IPN's electrical conductivity increases dramatically after adding GNP due to molecular rearrangements of the intertwined polymer chains that continuously distribute the GNP nanosheets, This new hydrophilic composite biomaterial film shows great promise for skin biomedical applications, especially those that require antiviral and/or biodegradable electroconductive materials.
36234870	4	0	theme	molecular	537:545	arg1	interactions					547:558	the molecular interactions	533:558	the molecular interactions between curcumin, keratin, and pectin	533:596	The structures and the molecular interactions between curcumin, keratin, and pectin were examined using Fourier transform infrared spectroscopy and X-ray diffraction, and the thermal properties were determined through thermogravimetric analysis.
36234870	6	1	theme	composite	1235:1243	arg1	films					1245:1249	composite films	1235:1249	composite films	1235:1249	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	2	2	theme	keratin/gelatin/glycerin/curcumin	340:372	arg1	films					384:388	keratin/gelatin/glycerin/curcumin composite films	340:388	keratin/gelatin/glycerin/curcumin composite films	340:388	Curcumin was successively incorporated in a mixed solution including keratin, gelatin, and glycerin to prepare different kinds of keratin/gelatin/glycerin/curcumin composite films.
36234870	6	3	theme	control	996:1002	arg1	group					1004:1008	the control group	992:1008	the control group (no film wrapped on the surface of tomato)	992:1051	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	7	4	theme	curcumin	1418:1425	arg1	addition					1406:1413	the addition	1402:1413	the addition of curcumin	1402:1425	Finally, the composite films have a superior antibacterial effect against Staphylococcus aureus and Escherichia coli because of the addition of curcumin.
36234870	7	5	contain	have	1303:1306	arg2	effect					1333:1338	a superior antibacterial effect	1308:1338	a superior antibacterial effect against Staphylococcus aureus and Escherichia coli	1308:1389	Finally, the composite films have a superior antibacterial effect against Staphylococcus aureus and Escherichia coli because of the addition of curcumin.
36234870	7	5	contain	have	1303:1306	arg1	films					1297:1301	the composite films	1283:1301	the composite films	1283:1301	Finally, the composite films have a superior antibacterial effect against Staphylococcus aureus and Escherichia coli because of the addition of curcumin.
36234870	5	6	theme	keratin/gelatin/curcumin	823:846	arg1	films					858:862	keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	785:862	keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	785:862	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	8	7	theme	curcumin	1452:1459	arg1	concentration					1435:1447	the concentration	1431:1447	the concentration of curcumin	1431:1459	As the concentration of curcumin reaches 1.0%, the antibacterial activity effect of the film is significantly improved.
36234870	9	8	theme	coli	1590:1593	arg1	zone					1579:1582	the inhibition zone	1564:1582	the inhibition zone of E. coli	1564:1593	The diameter of the inhibition zone of E. coli is (12.16 ± 0.53) mm, and that of S. aureus is (14.532 ± 0.97) mm.
36234870	6	9	theme	groups	1160:1165	arg1	±					1175:1175	8.76 ±	1170:1175	8.76 ± 0.2%	1170:1180	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	9	theme	groups	1160:1165	arg1	ratio					1058:1062	the ratio	1054:1062	the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1054:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	10	from	±	1175:1175	arg1	addition					968:975	addition	968:975	addition	968:975	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	11	dep	group	1004:1008	arg1	film					1014:1017	no film	1011:1017	no film wrapped on the surface of tomato	1011:1050	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	5	12	theme	composite	848:856	arg1	films					858:862	keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	785:862	keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	785:862	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	0	13	from	Effects	63:69	arg1	Tomato					81:86	Cherry Tomato	74:86	Cherry Tomato	74:86	Keratin-Based Composite Bioactive Films and Their Preservative Effects on Cherry Tomato.
36234870	6	14	theme	tomatoes	1213:1220	arg1	kg/cm3					1266:1271	11.2 ± 0.39 kg/cm3	1254:1271	11.2 ± 0.39 kg/cm3	1254:1271	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	14	theme	tomatoes	1213:1220	arg1	value					1200:1204	the hardness value	1187:1204	the hardness value of the tomatoes wrapped with composite films	1187:1249	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	15	theme	experimental	1147:1158	arg1	groups					1160:1165	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	16	theme	/glycerin	1114:1122	arg1	/curcumin					1130:1138	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin	1067:1138	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	16	theme	/glycerin	1114:1122	arg1	%					1144:1144	1.0%	1141:1144	1.0%	1141:1144	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	5	17	theme	films	858:862	arg1	strengths					772:780	The tensile strengths	760:780	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	760:862	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	5	17	theme	films	858:862	arg1	MPa					884:886	13.73 and 12.45 MPa	868:886	13.73 and 12.45 MPa	868:886	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	6	18	theme	keratin	1086:1092	arg1	%					1098:1098	7.0%	1095:1098	7.0%	1095:1098	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	18	theme	keratin	1086:1092	arg1	/gelatin					1100:1107	the keratin (7.0%)/gelatin	1082:1107	the keratin (7.0%)/gelatin	1082:1107	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	19	theme	loss	1074:1077	arg1	/glycerin					1114:1122	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin	1067:1122	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	19	theme	loss	1074:1077	arg1	%					1128:1128	2.0%	1125:1128	2.0%	1125:1128	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	4	20	theme	thermogravimetric	732:748	arg1	analysis					750:757	thermogravimetric analysis	732:757	thermogravimetric analysis	732:757	The structures and the molecular interactions between curcumin, keratin, and pectin were examined using Fourier transform infrared spectroscopy and X-ray diffraction, and the thermal properties were determined through thermogravimetric analysis.
36234870	6	21	theme	/gelatin	1100:1107	arg1	%					1112:1112	10%	1110:1112	10%	1110:1112	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	21	theme	/gelatin	1100:1107	arg1	loss					1074:1077	weight loss	1067:1077	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	22	theme	tomato	1045:1050	arg1	surface					1034:1040	the surface	1030:1040	the surface of tomato	1030:1050	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	5	23	theme	respective	913:922	arg1	%					953:953	56.7%	949:953	56.7%	949:953	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	5	23	theme	respective	913:922	arg1	elongations					924:934	their respective elongations	907:934	their respective elongations at break	907:943	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	1	24	theme	reduction	160:168	arg1	method					170:175	the reduction method	156:175	the reduction method using L-cysteine as a reductant	156:207	In this study, keratins were extracted from pig nail waste through the reduction method using L-cysteine as a reductant.
36234870	0	25	theme	Composite	14:22	arg1	Films					34:38	Keratin-Based Composite Bioactive Films	0:38	Keratin-Based Composite Bioactive Films	0:38	Keratin-Based Composite Bioactive Films and Their Preservative Effects on Cherry Tomato.
36234870	9	26	dep	mm	1613:1614	arg1	±					1605:1605	12.16 ± 0.53	1599:1610	12.16 ± 0.53	1599:1610	The diameter of the inhibition zone of E. coli is (12.16 ± 0.53) mm, and that of S. aureus is (14.532 ± 0.97) mm.
36234870	8	27	theme	activity	1493:1500	arg1	effect					1502:1507	the antibacterial activity effect	1475:1507	the antibacterial activity effect of the film	1475:1519	As the concentration of curcumin reaches 1.0%, the antibacterial activity effect of the film is significantly improved.
36234870	3	28	theme	films	458:462	arg1	morphology					395:404	The morphology	391:404	The morphology of the keratin/ gelatin/glycerin/curcumin composite films	391:462	The morphology of the keratin/ gelatin/glycerin/curcumin composite films were examined using scanning electron microscopy.
36234870	0	29	theme	Keratin-Based	0:12	arg1	Films					34:38	Keratin-Based Composite Bioactive Films	0:38	Keratin-Based Composite Bioactive Films	0:38	Keratin-Based Composite Bioactive Films and Their Preservative Effects on Cherry Tomato.
36234870	6	30	from	kg/cm3	1266:1271	arg1	addition					968:975	addition	968:975	addition	968:975	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	31	theme	hardness	1191:1198	arg1	kg/cm3					1266:1271	11.2 ± 0.39 kg/cm3	1254:1271	11.2 ± 0.39 kg/cm3	1254:1271	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	31	theme	hardness	1191:1198	arg1	value					1200:1204	the hardness value	1187:1204	the hardness value of the tomatoes wrapped with composite films	1187:1249	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	8	32	theme	antibacterial	1479:1491	arg1	effect					1502:1507	the antibacterial activity effect	1475:1507	the antibacterial activity effect of the film	1475:1519	As the concentration of curcumin reaches 1.0%, the antibacterial activity effect of the film is significantly improved.
36234870	6	33	theme	/curcumin	1130:1138	arg1	groups					1160:1165	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	10	34	theme	bioactive	1716:1724	arg1	films					1726:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	0	35	theme	Bioactive	24:32	arg1	Films					34:38	Keratin-Based Composite Bioactive Films	0:38	Keratin-Based Composite Bioactive Films	0:38	Keratin-Based Composite Bioactive Films and Their Preservative Effects on Cherry Tomato.
36234870	10	36	theme	food	1772:1775	arg1	industry					1787:1794	the food packaging industry	1768:1794	the food packaging industry	1768:1794	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	1	37	theme	pig	133:135	arg1	waste					142:146	pig nail waste	133:146	pig nail waste	133:146	In this study, keratins were extracted from pig nail waste through the reduction method using L-cysteine as a reductant.
36234870	3	38	theme	gelatin/glycerin/curcumin	422:446	arg1	films					458:462	the keratin/ gelatin/glycerin/curcumin composite films	409:462	the keratin/ gelatin/glycerin/curcumin composite films	409:462	The morphology of the keratin/ gelatin/glycerin/curcumin composite films were examined using scanning electron microscopy.
36234870	5	39	theme	keratin/gelatin/glycerin/curcumin	785:817	arg1	films					858:862	keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	785:862	keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	785:862	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	7	40	theme	superior	1310:1317	arg1	effect					1333:1338	a superior antibacterial effect	1308:1338	a superior antibacterial effect against Staphylococcus aureus and Escherichia coli	1308:1389	Finally, the composite films have a superior antibacterial effect against Staphylococcus aureus and Escherichia coli because of the addition of curcumin.
36234870	3	41	theme	scanning	484:491	arg1	microscopy					502:511	scanning electron microscopy	484:511	scanning electron microscopy	484:511	The morphology of the keratin/ gelatin/glycerin/curcumin composite films were examined using scanning electron microscopy.
36234870	3	42	theme	composite	448:456	arg1	films					458:462	the keratin/ gelatin/glycerin/curcumin composite films	409:462	the keratin/ gelatin/glycerin/curcumin composite films	409:462	The morphology of the keratin/ gelatin/glycerin/curcumin composite films were examined using scanning electron microscopy.
36234870	8	43	theme	film	1516:1519	arg1	effect					1502:1507	the antibacterial activity effect	1475:1507	the antibacterial activity effect of the film	1475:1519	As the concentration of curcumin reaches 1.0%, the antibacterial activity effect of the film is significantly improved.
36234870	10	44	contain	have	1732:1735	arg2	application					1753:1763	great potential application	1737:1763	great potential application	1737:1763	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	10	44	contain	have	1732:1735	arg1	films					1726:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	4	45	dep	transform	626:634	arg1	infrared					636:643	infrared	636:643	transform infrared spectroscopy and X-ray diffraction	626:678	The structures and the molecular interactions between curcumin, keratin, and pectin were examined using Fourier transform infrared spectroscopy and X-ray diffraction, and the thermal properties were determined through thermogravimetric analysis.
36234870	7	46	theme	antibacterial	1319:1331	arg1	effect					1333:1338	a superior antibacterial effect	1308:1338	a superior antibacterial effect against Staphylococcus aureus and Escherichia coli	1308:1389	Finally, the composite films have a superior antibacterial effect against Staphylococcus aureus and Escherichia coli because of the addition of curcumin.
36234870	4	47	theme	thermal	689:695	arg1	properties					697:706	the thermal properties	685:706	the thermal properties	685:706	The structures and the molecular interactions between curcumin, keratin, and pectin were examined using Fourier transform infrared spectroscopy and X-ray diffraction, and the thermal properties were determined through thermogravimetric analysis.
36234870	9	48	dep	mm	1658:1659	arg1	±					1650:1650	14.532 ± 0.97	1643:1655	14.532 ± 0.97	1643:1655	The diameter of the inhibition zone of E. coli is (12.16 ± 0.53) mm, and that of S. aureus is (14.532 ± 0.97) mm.
36234870	4	49	dep	Fourier	618:624	arg1	transform					626:634	transform	626:634	transform infrared spectroscopy and X-ray diffraction	626:678	The structures and the molecular interactions between curcumin, keratin, and pectin were examined using Fourier transform infrared spectroscopy and X-ray diffraction, and the thermal properties were determined through thermogravimetric analysis.
36234870	10	50	theme	multifunctional	1666:1680	arg1	films					1726:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	0	51	theme	Preservative	50:61	arg1	Effects					63:69	Their Preservative Effects	44:69	Their Preservative Effects on Cherry Tomato	44:86	Keratin-Based Composite Bioactive Films and Their Preservative Effects on Cherry Tomato.
36234870	10	52	theme	potential	1743:1751	arg1	application					1753:1763	great potential application	1737:1763	great potential application	1737:1763	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	6	53	theme	%	1180:1180	arg1	±					1175:1175	8.76 ±	1170:1175	8.76 ± 0.2%	1170:1180	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	53	theme	%	1180:1180	arg1	ratio					1058:1062	the ratio	1054:1062	the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1054:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	5	54	from	break	939:943	arg1	%					953:953	56.7%	949:953	56.7%	949:953	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	5	54	from	break	939:943	arg1	elongations					924:934	their respective elongations	907:934	their respective elongations at break	907:943	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	2	55	theme	different	321:329	arg1	kinds					331:335	different kinds	321:335	different kinds of keratin/gelatin/glycerin/curcumin composite films	321:388	Curcumin was successively incorporated in a mixed solution including keratin, gelatin, and glycerin to prepare different kinds of keratin/gelatin/glycerin/curcumin composite films.
36234870	6	56	theme	0.2	1177:1179	arg1	%					1180:1180	%	1180:1180	%	1180:1180	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	2	57	theme	mixed	254:258	arg1	solution					260:267	a mixed solution	252:267	a mixed solution including keratin, gelatin, and glycerin to prepare different kinds of keratin/gelatin/glycerin/curcumin composite films	252:388	Curcumin was successively incorporated in a mixed solution including keratin, gelatin, and glycerin to prepare different kinds of keratin/gelatin/glycerin/curcumin composite films.
36234870	10	58	theme	keratin/gelatin/glycerin/curcumin	1682:1714	arg1	films					1726:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films	1662:1730	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	4	59	theme	X-ray	662:666	arg1	diffraction					668:678	X-ray diffraction	662:678	X-ray diffraction	662:678	The structures and the molecular interactions between curcumin, keratin, and pectin were examined using Fourier transform infrared spectroscopy and X-ray diffraction, and the thermal properties were determined through thermogravimetric analysis.
36234870	5	60	theme	tensile	764:770	arg1	strengths					772:780	The tensile strengths	760:780	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films	760:862	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	5	60	theme	tensile	764:770	arg1	MPa					884:886	13.73 and 12.45 MPa	868:886	13.73 and 12.45 MPa	868:886	The tensile strengths of keratin/gelatin/glycerin/curcumin and keratin/gelatin/curcumin composite films are 13.73 and 12.45 MPa, respectively, and their respective elongations at break are 56.7% and 4.6%.
36234870	7	61	theme	composite	1287:1295	arg1	films					1297:1301	the composite films	1283:1301	the composite films	1283:1301	Finally, the composite films have a superior antibacterial effect against Staphylococcus aureus and Escherichia coli because of the addition of curcumin.
36234870	1	62	theme	nail	137:140	arg1	waste					142:146	pig nail waste	133:146	pig nail waste	133:146	In this study, keratins were extracted from pig nail waste through the reduction method using L-cysteine as a reductant.
36234870	6	63	theme	weight	1067:1072	arg1	%					1112:1112	10%	1110:1112	10%	1110:1112	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	6	63	theme	weight	1067:1072	arg1	loss					1074:1077	weight loss	1067:1077	weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups	1067:1165	In addition, compared with the control group (no film wrapped on the surface of tomato), the ratio of weight loss of the keratin (7.0%)/gelatin (10%)/glycerin (2.0%)/curcumin (1.0%) experimental groups is 8.76 ± 0.2%, and the hardness value of the tomatoes wrapped with composite films is 11.2 ± 0.39 kg/cm3.
36234870	0	64	theme	Cherry	74:79	arg1	Tomato					81:86	Cherry Tomato	74:86	Cherry Tomato	74:86	Keratin-Based Composite Bioactive Films and Their Preservative Effects on Cherry Tomato.
36234870	3	65	theme	electron	493:500	arg1	microscopy					502:511	scanning electron microscopy	484:511	scanning electron microscopy	484:511	The morphology of the keratin/ gelatin/glycerin/curcumin composite films were examined using scanning electron microscopy.
36234870	9	66	theme	inhibition	1568:1577	arg1	zone					1579:1582	the inhibition zone	1564:1582	the inhibition zone of E. coli	1564:1593	The diameter of the inhibition zone of E. coli is (12.16 ± 0.53) mm, and that of S. aureus is (14.532 ± 0.97) mm.
36234870	2	67	theme	films	384:388	arg1	kinds					331:335	different kinds	321:335	different kinds of keratin/gelatin/glycerin/curcumin composite films	321:388	Curcumin was successively incorporated in a mixed solution including keratin, gelatin, and glycerin to prepare different kinds of keratin/gelatin/glycerin/curcumin composite films.
36234870	10	68	theme	packaging	1777:1785	arg1	industry					1787:1794	the food packaging industry	1768:1794	the food packaging industry	1768:1794	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36234870	0	69	from	Films	34:38	arg1	Tomato					81:86	Cherry Tomato	74:86	Cherry Tomato	74:86	Keratin-Based Composite Bioactive Films and Their Preservative Effects on Cherry Tomato.
36234870	9	70	theme	zone	1579:1582	arg1	diameter					1552:1559	The diameter	1548:1559	The diameter of the inhibition zone of E. coli	1548:1593	The diameter of the inhibition zone of E. coli is (12.16 ± 0.53) mm, and that of S. aureus is (14.532 ± 0.97) mm.
36234870	9	70	theme	zone	1579:1582	arg1	mm					1613:1614	mm	1613:1614	mm	1613:1614	The diameter of the inhibition zone of E. coli is (12.16 ± 0.53) mm, and that of S. aureus is (14.532 ± 0.97) mm.
36234870	2	71	theme	composite	374:382	arg1	films					384:388	keratin/gelatin/glycerin/curcumin composite films	340:388	keratin/gelatin/glycerin/curcumin composite films	340:388	Curcumin was successively incorporated in a mixed solution including keratin, gelatin, and glycerin to prepare different kinds of keratin/gelatin/glycerin/curcumin composite films.
36234870	10	72	theme	great	1737:1741	arg1	application					1753:1763	great potential application	1737:1763	great potential application	1737:1763	The multifunctional keratin/gelatin/glycerin/curcumin bioactive films have great potential application in the food packaging industry.
36111808	0	0	theme	supplementation	61:75	arg1	Effects					0:6	Effects	0:6	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.	0:142	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	2	1	theme	inflammatory	458:469	arg1	disease					477:483	inflammatory bowel disease	458:483	inflammatory bowel disease	458:483	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	6	2	theme	TGR	1078:1080	arg1	supplementation					1082:1096	</b> Daily TGR supplementation	1067:1096	</b> Daily TGR supplementation	1067:1096	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	2	3	theme	<i>in	313:317	arg1	simulation					329:338	</b> A mucosal <i>in vitro</i> simulation	298:338	</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	298:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	5	4	from	production	1001:1010	arg1	coculture					1042:1050	Caco-2/THP1 coculture	1030:1050	Caco-2/THP1 coculture	1030:1050	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	1	5	theme	system	210:215	arg1	supplementation					217:231	the Total Gut Restoration (TGR) system supplementation	178:231	the Total Gut Restoration (TGR) system supplementation	178:231	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	0	6	theme	acid	56:59	arg1	supplementation					61:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	4	7	from	increase	776:783	arg1	production					811:820	short-chain fatty acid production	788:820	short-chain fatty acid production	788:820	<b>Results:</b> Compared with unsupplemented controls, supplementation was associated with a significant increase in short-chain fatty acid production, and changes to the microbiome were observed.
36111808	2	8	theme	mucosal	305:311	arg1	simulation					329:338	</b> A mucosal <i>in vitro</i> simulation	298:338	</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	298:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	1	9	theme	supplementation	217:231	arg1	effects					167:173	The effects	163:173	The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome	163:253	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	0	10	from	Effects	0:6	arg1	microbiome					88:97	the gut microbiome	80:97	the gut microbiome of patients with inflammatory bowel disease	80:141	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	4	11	theme	unsupplemented	701:714	arg1	controls					716:723	unsupplemented controls	701:723	unsupplemented controls	701:723	<b>Results:</b> Compared with unsupplemented controls, supplementation was associated with a significant increase in short-chain fatty acid production, and changes to the microbiome were observed.
36111808	5	12	from	chambers	899:906	arg1	Supernatants					868:879	Supernatants	868:879	Supernatants from supplemented chambers	868:906	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	5	13	theme	MCP1	996:999	arg1	production					1001:1010	MCP1 production	996:1010	MCP1 production	996:1010	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	2	14	theme	gastrointestinal	353:368	arg1	system					398:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	0	15	theme	gut	84:86	arg1	microbiome					88:97	the gut microbiome	80:97	the gut microbiome of patients with inflammatory bowel disease	80:141	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	2	16	from	patients	444:451	arg1	samples					431:437	fecal samples	425:437	fecal samples from patients with inflammatory bowel disease	425:483	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	4	17	theme	significant	764:774	arg1	increase					776:783	a significant increase	762:783	a significant increase in short-chain fatty acid production	762:820	<b>Results:</b> Compared with unsupplemented controls, supplementation was associated with a significant increase in short-chain fatty acid production, and changes to the microbiome were observed.
36111808	6	18	theme	gut	1125:1127	arg1	microbiome					1129:1138	the gut microbiome	1121:1138	the gut microbiome of patients with inflammatory bowel disease	1121:1182	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	2	19	theme	human	347:351	arg1	system					398:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	5	20	theme	IL-6	956:959	arg1	production					971:980	IL-6 and IL-10 production	956:980	IL-6 and IL-10 production	956:980	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	1	21	theme	gut	240:242	arg1	microbiome					244:253	the gut microbiome	236:253	the gut microbiome	236:253	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	3	22	theme	prebiotic	648:656	arg1	flavonoids					658:667	prebiotic flavonoids	648:667	prebiotic flavonoids	648:667	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	2	23	dep	methods	290:296	arg1	&amp					284:287	<b>Materials &amp	271:287	<b>Materials &amp	271:287	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	0	24	theme	prebiotic	20:28	arg1	supplementation					61:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	4	25	theme	acid	806:809	arg1	production					811:820	short-chain fatty acid production	788:820	short-chain fatty acid production	788:820	<b>Results:</b> Compared with unsupplemented controls, supplementation was associated with a significant increase in short-chain fatty acid production, and changes to the microbiome were observed.
36111808	0	26	theme	patients	102:109	arg1	microbiome					88:97	the gut microbiome	80:97	the gut microbiome of patients with inflammatory bowel disease	80:141	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	2	27	theme	<b>Materials	271:282	arg1	&amp					284:287	<b>Materials &amp	271:287	<b>Materials &amp	271:287	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	0	28	theme	combined	11:18	arg1	supplementation					61:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	3	29	theme	amino	632:636	arg1	acids					638:642	amino acids	632:642	amino acids	632:642	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	5	30	theme	intestinal	917:926	arg1	function					936:943	intestinal barrier function	917:943	intestinal barrier function	917:943	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	2	31	theme	M-SHIME<sup>®</sup>	377:395	arg1	system					398:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	0	32	theme	probiotic	31:39	arg1	supplementation					61:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	5	33	theme	Caco-2/THP1	1030:1040	arg1	coculture					1042:1050	Caco-2/THP1 coculture	1030:1050	Caco-2/THP1 coculture	1030:1050	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	0	34	theme	inflammatory	116:127	arg1	disease					135:141	inflammatory bowel disease	116:141	inflammatory bowel disease	116:141	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	6	35	theme	Daily	1072:1076	arg1	supplementation					1082:1096	</b> Daily TGR supplementation	1067:1096	</b> Daily TGR supplementation	1067:1096	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	1	36	theme	Total	182:186	arg1	system					210:215	the Total Gut Restoration (TGR) system	178:215	the Total Gut Restoration (TGR) system supplementation	178:231	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	1	37	theme	Gut	188:190	arg1	system					210:215	the Total Gut Restoration (TGR) system	178:215	the Total Gut Restoration (TGR) system supplementation	178:231	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	0	38	theme	IgG	42:44	arg1	supplementation					61:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	6	39	theme	</b>	1067:1070	arg1	supplementation					1082:1096	</b> Daily TGR supplementation	1067:1096	</b> Daily TGR supplementation	1067:1096	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	3	40	theme	probiotic	550:558	arg1	strains					576:582	five probiotic <i>Bacillus</i> strains	545:582	five probiotic <i>Bacillus</i> strains	545:582	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	6	41	theme	patients	1143:1150	arg1	microbiome					1129:1138	the gut microbiome	1121:1138	the gut microbiome of patients with inflammatory bowel disease	1121:1182	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	5	42	theme	barrier	928:934	arg1	function					936:943	intestinal barrier function	917:943	intestinal barrier function	917:943	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	2	43	theme	fecal	425:429	arg1	samples					431:437	fecal samples	425:437	fecal samples from patients with inflammatory bowel disease	425:483	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	6	44	with	patients	1143:1150	arg1	disease					1176:1182	inflammatory bowel disease	1157:1182	inflammatory bowel disease	1157:1182	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	0	45	theme	bowel	129:133	arg1	disease					135:141	inflammatory bowel disease	116:141	inflammatory bowel disease	116:141	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	3	46	theme	<i>Bacillus</i>	560:574	arg1	strains					576:582	five probiotic <i>Bacillus</i> strains	545:582	five probiotic <i>Bacillus</i> strains	545:582	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	6	47	theme	bowel	1170:1174	arg1	disease					1176:1182	inflammatory bowel disease	1157:1182	inflammatory bowel disease	1157:1182	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	4	48	theme	fatty	800:804	arg1	acid					806:809	short-chain fatty acid	788:809	short-chain fatty acid production	788:820	<b>Results:</b> Compared with unsupplemented controls, supplementation was associated with a significant increase in short-chain fatty acid production, and changes to the microbiome were observed.
36111808	5	49	theme	IL-10	965:969	arg1	production					971:980	IL-6 and IL-10 production	956:980	IL-6 and IL-10 production	956:980	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	4	50	theme	short-chain	788:798	arg1	acid					806:809	short-chain fatty acid	788:809	short-chain fatty acid production	788:820	<b>Results:</b> Compared with unsupplemented controls, supplementation was associated with a significant increase in short-chain fatty acid production, and changes to the microbiome were observed.
36111808	6	51	theme	inflammatory	1157:1168	arg1	disease					1176:1182	inflammatory bowel disease	1157:1182	inflammatory bowel disease	1157:1182	<b>Conclusion:</b> Daily TGR supplementation facilitated changes to the gut microbiome of patients with inflammatory bowel disease.
36111808	3	52	theme	TGR	533:535	arg1	system					537:542	the TGR system	529:542	the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids)	529:668	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	1	53	theme	Restoration	192:202	arg1	system					210:215	the Total Gut Restoration (TGR) system	178:215	the Total Gut Restoration (TGR) system supplementation	178:231	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	3	54	theme	immunoglobulin	604:617	arg1	concentrate					619:629	immunoglobulin concentrate	604:629	immunoglobulin concentrate	604:629	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	3	55	dep	system	537:542	arg1	strains					576:582	five probiotic <i>Bacillus</i> strains	545:582	five probiotic <i>Bacillus</i> strains	545:582	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	3	55	dep	system	537:542	arg1	mixture					595:601	prebiotic mixture	585:601	prebiotic mixture	585:601	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	3	55	dep	system	537:542	arg1	concentrate					619:629	immunoglobulin concentrate	604:629	immunoglobulin concentrate	604:629	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	3	55	dep	system	537:542	arg1	acids					638:642	amino acids	632:642	amino acids	632:642	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	3	55	dep	system	537:542	arg1	flavonoids					658:667	prebiotic flavonoids	648:667	prebiotic flavonoids	648:667	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	0	56	with	patients	102:109	arg1	disease					135:141	inflammatory bowel disease	116:141	inflammatory bowel disease	116:141	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	0	57	theme	amino	50:54	arg1	supplementation					61:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	combined prebiotic, probiotic, IgG and amino acid supplementation	11:75	Effects of combined prebiotic, probiotic, IgG and amino acid supplementation on the gut microbiome of patients with inflammatory bowel disease.
36111808	2	58	theme	tract	370:374	arg1	system					398:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	343:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	3	59	theme	prebiotic	585:593	arg1	mixture					595:601	prebiotic mixture	585:601	prebiotic mixture	585:601	Chambers were supplemented for 5 days with the TGR system (five probiotic <i>Bacillus</i> strains, prebiotic mixture, immunoglobulin concentrate, amino acids and prebiotic flavonoids).
36111808	1	60	from	effects	167:173	arg1	microbiome					244:253	the gut microbiome	236:253	the gut microbiome	236:253	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	5	61	theme	supplemented	886:897	arg1	chambers					899:906	supplemented chambers	886:906	supplemented chambers	886:906	Supernatants from supplemented chambers improved intestinal barrier function, increased IL-6 and IL-10 production and decreased MCP1 production versus control in Caco-2/THP1 coculture.
36111808	1	62	theme	TGR	205:207	arg1	system					210:215	the Total Gut Restoration (TGR) system	178:215	the Total Gut Restoration (TGR) system supplementation	178:231	<b>Background:</b> The effects of the Total Gut Restoration (TGR) system supplementation on the gut microbiome were evaluated.
36111808	2	63	theme	bowel	471:475	arg1	disease					477:483	inflammatory bowel disease	458:483	inflammatory bowel disease	458:483	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	2	64	theme	system	398:403	arg1	simulation					329:338	</b> A mucosal <i>in vitro</i> simulation	298:338	</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	298:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	2	65	with	patients	444:451	arg1	disease					477:483	inflammatory bowel disease	458:483	inflammatory bowel disease	458:483	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36111808	2	66	theme	vitro</i>	319:327	arg1	simulation					329:338	</b> A mucosal <i>in vitro</i> simulation	298:338	</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system	298:403	<b>Materials &amp; methods:</b> A mucosal <i>in vitro</i> simulation of the human gastrointestinal tract (M-SHIME<sup>®</sup>) system was inoculated with fecal samples from patients with inflammatory bowel disease.
36145094	6	0	theme	fat	1049:1051	arg1	digestion					1025:1033	the digestion	1021:1033	the digestion of starch and fat	1021:1051	In addition, P-PCs exhibited strong inhibitory on the digestion of starch and fat.
36145094	3	1	theme	high-fat	517:524	arg1	diet					526:529	high-fat diet	517:529	high-fat diet induced obesity	517:545	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	6	2	theme	starch	1038:1043	arg1	digestion					1025:1033	the digestion	1021:1033	the digestion of starch and fat	1021:1051	In addition, P-PCs exhibited strong inhibitory on the digestion of starch and fat.
36145094	3	3	from	effects	420:426	arg1	diet					526:529	high-fat diet	517:529	high-fat diet induced obesity	517:545	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	0	4	theme	High	110:113	arg1	Diet					119:122	High Fat Diet	110:122	High Fat Diet	110:122	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	0	5	contain	Possess	69:75	arg1	Proanthocyanidins					10:26	Persimmon Proanthocyanidins	0:26	Persimmon Proanthocyanidins with Different Degrees of Polymerization	0:67	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	0	5	contain	Possess	69:75	arg2	Activities					86:95	Distinct Activities	77:95	Distinct Activities in Models of High Fat Diet Induced Obesity	77:138	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	4	6	theme	insulin	796:802	arg1	resistance					804:813	insulin resistance	796:813	insulin resistance	796:813	The findings indicated that both of P-PCs and P-OPCs significantly reduced the body weight, and P-PCs showed stronger anti-obesity ability compared with P-OPCs, P-OPCs seemed with stronger ability on improvement of insulin resistance.
36145094	5	7	from	P-PCs	923:927	arg1	mice					965:968	C57BL/6J mice	956:968	C57BL/6J mice	956:968	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	5	8	theme	P-OPCs	933:938	arg1	intervention					940:951	P-OPCs intervention	933:951	P-OPCs intervention	933:951	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	4	9	theme	body	660:663	arg1	weight					665:670	the body weight	656:670	the body weight	656:670	The findings indicated that both of P-PCs and P-OPCs significantly reduced the body weight, and P-PCs showed stronger anti-obesity ability compared with P-OPCs, P-OPCs seemed with stronger ability on improvement of insulin resistance.
36145094	4	10	theme	anti-obesity	699:710	arg1	ability					712:718	stronger anti-obesity ability	690:718	stronger anti-obesity ability	690:718	The findings indicated that both of P-PCs and P-OPCs significantly reduced the body weight, and P-PCs showed stronger anti-obesity ability compared with P-OPCs, P-OPCs seemed with stronger ability on improvement of insulin resistance.
36145094	1	11	theme	polyphenol	172:181	arg1	Proanthocyanidins					141:157	Proanthocyanidins	141:157	Proanthocyanidins	141:157	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	1	11	theme	polyphenol	172:181	arg1	kind					164:167	a kind	162:167	a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity	162:265	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	0	12	theme	Fat	115:117	arg1	Diet					119:122	High Fat Diet	110:122	High Fat Diet	110:122	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	4	13	theme	resistance	804:813	arg1	improvement					781:791	improvement	781:791	improvement of insulin resistance	781:813	The findings indicated that both of P-PCs and P-OPCs significantly reduced the body weight, and P-PCs showed stronger anti-obesity ability compared with P-OPCs, P-OPCs seemed with stronger ability on improvement of insulin resistance.
36145094	7	14	theme	stronger	1104:1111	arg1	ability					1126:1132	stronger anti-obesity ability	1104:1132	stronger anti-obesity ability	1104:1132	Above all, this study indicated that P-PCs showed stronger anti-obesity ability compared with P-OPCs.
36145094	3	15	theme	persimmon	431:439	arg1	P-PCs					460:464	P-PCs	460:464	P-PCs	460:464	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	3	15	theme	persimmon	431:439	arg1	proanthocyanidins					441:457	persimmon proanthocyanidins	431:457	persimmon proanthocyanidins (P-PCs)	431:465	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	0	16	theme	Persimmon	0:8	arg1	Proanthocyanidins					10:26	Persimmon Proanthocyanidins	0:26	Persimmon Proanthocyanidins with Different Degrees of Polymerization	0:67	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	7	17	theme	anti-obesity	1113:1124	arg1	ability					1126:1132	stronger anti-obesity ability	1104:1132	stronger anti-obesity ability	1104:1132	Above all, this study indicated that P-PCs showed stronger anti-obesity ability compared with P-OPCs.
36145094	2	18	theme	polymerization	318:331	arg1	degree					333:338	different polymerization degree	308:338	different polymerization degree	308:338	However, whether proanthocyanidins with different polymerization degree showed different anti-obesity ability is unclear.
36145094	3	19	theme	proanthocyanidins	441:457	arg1	effects					420:426	the effects	416:426	the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity	416:545	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	0	20	theme	Different	33:41	arg1	Degrees					43:49	Different Degrees	33:49	Different Degrees of Polymerization	33:67	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	0	21	theme	Obesity	132:138	arg1	Models					100:105	Models	100:105	Models of High Fat Diet Induced Obesity	100:138	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	2	22	theme	different	308:316	arg1	degree					333:338	different polymerization degree	308:338	different polymerization degree	308:338	However, whether proanthocyanidins with different polymerization degree showed different anti-obesity ability is unclear.
36145094	3	23	theme	persimmon	471:479	arg1	P-OPCs					506:511	P-OPCs	506:511	P-OPCs	506:511	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	3	23	theme	persimmon	471:479	arg1	oligo-proanthocyanidins					481:503	persimmon oligo-proanthocyanidins	471:503	persimmon oligo-proanthocyanidins (P-OPCs)	471:512	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	4	24	theme	stronger	761:768	arg1	ability					770:776	stronger ability	761:776	stronger ability	761:776	The findings indicated that both of P-PCs and P-OPCs significantly reduced the body weight, and P-PCs showed stronger anti-obesity ability compared with P-OPCs, P-OPCs seemed with stronger ability on improvement of insulin resistance.
36145094	0	25	theme	Induced	124:130	arg1	Obesity					132:138	High Fat Diet Induced Obesity	110:138	High Fat Diet Induced Obesity	110:138	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	3	26	theme	oligo-proanthocyanidins	481:503	arg1	effects					420:426	the effects	416:426	the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity	416:545	Therefore, in this study, the effects of persimmon proanthocyanidins (P-PCs) and persimmon oligo-proanthocyanidins (P-OPCs) on high-fat diet induced obesity were systematically investigated.
36145094	5	27	theme	C57BL/6J	956:963	arg1	mice					965:968	C57BL/6J mice	956:968	C57BL/6J mice	956:968	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	4	28	theme	stronger	690:697	arg1	ability					712:718	stronger anti-obesity ability	690:718	stronger anti-obesity ability	690:718	The findings indicated that both of P-PCs and P-OPCs significantly reduced the body weight, and P-PCs showed stronger anti-obesity ability compared with P-OPCs, P-OPCs seemed with stronger ability on improvement of insulin resistance.
36145094	1	29	theme	strong	208:213	arg1	ability					226:232	strong prevention ability	208:232	strong prevention ability	208:232	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	5	30	theme	microbiota	894:903	arg1	composition					871:881	the composition	867:881	the composition of the gut microbiota	867:903	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	0	31	from	Activities	86:95	arg1	Models					100:105	Models	100:105	Models of High Fat Diet Induced Obesity	100:138	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	2	32	with	proanthocyanidins	285:301	arg1	degree					333:338	different polymerization degree	308:338	different polymerization degree	308:338	However, whether proanthocyanidins with different polymerization degree showed different anti-obesity ability is unclear.
36145094	1	33	theme	prevention	215:224	arg1	ability					226:232	strong prevention ability	208:232	strong prevention ability	208:232	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	5	34	theme	gut	829:831	arg1	results					844:850	gut microbiota results	829:850	gut microbiota results	829:850	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	5	35	theme	microbiota	833:842	arg1	results					844:850	gut microbiota results	829:850	gut microbiota results	829:850	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	0	36	theme	Polymerization	54:67	arg1	Degrees					43:49	Different Degrees	33:49	Different Degrees of Polymerization	33:67	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	0	37	with	Proanthocyanidins	10:26	arg1	Degrees					43:49	Different Degrees	33:49	Different Degrees of Polymerization	33:67	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	2	38	theme	anti-obesity	357:368	arg1	ability					370:376	different anti-obesity ability	347:376	different anti-obesity ability	347:376	However, whether proanthocyanidins with different polymerization degree showed different anti-obesity ability is unclear.
36145094	1	39	located	found	197:201	arg2	kind					164:167	a kind	162:167	a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity	162:265	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	1	39	located	found	197:201	arg2	Proanthocyanidins					141:157	Proanthocyanidins	141:157	Proanthocyanidins	141:157	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	1	39	located	found	197:201	arg1	diet					246:249	high fat diet	237:249	high fat diet induced obesity	237:265	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	5	40	from	intervention	940:951	arg1	mice					965:968	C57BL/6J mice	956:968	C57BL/6J mice	956:968	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	2	41	theme	different	347:355	arg1	ability					370:376	different anti-obesity ability	347:376	different anti-obesity ability	347:376	However, whether proanthocyanidins with different polymerization degree showed different anti-obesity ability is unclear.
36145094	5	42	theme	gut	890:892	arg1	microbiota					894:903	the gut microbiota	886:903	the gut microbiota	886:903	Furthermore, gut microbiota results indicated that the composition of the gut microbiota was changed after P-PCs and P-OPCs intervention in C57BL/6J mice.
36145094	1	43	theme	high	237:240	arg1	diet					246:249	high fat diet	237:249	high fat diet induced obesity	237:265	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36145094	0	44	theme	Distinct	77:84	arg1	Activities					86:95	Distinct Activities	77:95	Distinct Activities in Models of High Fat Diet Induced Obesity	77:138	Persimmon Proanthocyanidins with Different Degrees of Polymerization Possess Distinct Activities in Models of High Fat Diet Induced Obesity.
36145094	1	45	theme	fat	242:244	arg1	diet					246:249	high fat diet	237:249	high fat diet induced obesity	237:265	Proanthocyanidins is a kind of polyphenol that had been found with strong prevention ability on high fat diet induced obesity.
36118209	0	0	theme	evolution	90:98	arg1	patterns					63:70	the patterns	59:70	the patterns of gut microbiota evolution among the widespread Asian honeybee populations	59:146	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	4	1	theme	community	914:922	arg1	diversity					891:899	the diversity	887:899	the diversity of microbial community among A. cerana populations	887:950	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	0	2	theme	microbiota	79:88	arg1	evolution					90:98	gut microbiota evolution	75:98	gut microbiota evolution	75:98	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	5	3	theme	microbiome	1070:1079	arg1	function					1050:1057	function	1050:1057	function	1050:1057	Pollen diets were significantly correlated with both the composition and function of the gut microbiome.
36118209	5	3	theme	microbiome	1070:1079	arg1	composition					1034:1044	composition	1034:1044	composition	1034:1044	Pollen diets were significantly correlated with both the composition and function of the gut microbiome.
36118209	2	4	theme	environmental	513:525	arg1	heterogeneity					527:539	environmental heterogeneity	513:539	environmental heterogeneity	513:539	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	4	5	theme	sequence-discrete	787:803	arg1	SDP					817:819	SDP	817:819	SDP	817:819	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	4	5	theme	sequence-discrete	787:803	arg1	population					805:814	sequence-discrete population	787:814	sequence-discrete population (SDP)	787:820	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	4	6	dep	extensive	851:859	arg1	overlaps					861:868	overlaps	861:868	overlaps	861:868	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	7	7	theme	feeding	1244:1250	arg1	bioassays					1268:1276	feeding and inoculation bioassays	1244:1276	feeding and inoculation bioassays	1244:1276	By feeding and inoculation bioassays, we confirmed that the variations in pollen polysaccharide composition contributed to the trade-off of these core bacteria.
36118209	9	8	theme	environmental	1594:1606	arg1	factors					1608:1614	environmental factors	1594:1614	environmental factors	1594:1614	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	2	9	theme	Apis	286:289	arg1	mellifera					291:299	the western honeybee Apis mellifera	265:299	the western honeybee Apis mellifera	265:299	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	0	10	theme	Asian	121:125	arg1	populations					136:146	the widespread Asian honeybee populations	106:146	the widespread Asian honeybee populations	106:146	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	2	11	theme	large	544:548	arg1	scales					561:566	large geographic scales	544:566	large geographic scales	544:566	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	2	12	theme	honeybee	277:284	arg1	mellifera					291:299	the western honeybee Apis mellifera	265:299	the western honeybee Apis mellifera	265:299	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	1	13	theme	crucial	173:179	arg1	microbiome					157:166	The gut microbiome	149:166	The gut microbiome	149:166	The gut microbiome is a crucial element that facilitates a host's adaptation to a changing environment.
36118209	1	13	theme	crucial	173:179	arg1	element					181:187	a crucial element	171:187	a crucial element that facilitates a host's adaptation to a changing environment	171:250	The gut microbiome is a crucial element that facilitates a host's adaptation to a changing environment.
36118209	0	14	theme	widespread	110:119	arg1	populations					136:146	the widespread Asian honeybee populations	106:146	the widespread Asian honeybee populations	106:146	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	8	15	theme	Progressive	1402:1412	arg1	change					1414:1419	Progressive change	1402:1419	Progressive change	1402:1419	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	8	15	theme	Progressive	1402:1412	arg1	foundation					1447:1456	the foundation	1443:1456	the foundation of gut microbiome evolution among the Asian honeybee	1443:1509	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	7	16	from	variations	1301:1310	arg1	composition					1337:1347	pollen polysaccharide composition	1315:1347	pollen polysaccharide composition	1315:1347	By feeding and inoculation bioassays, we confirmed that the variations in pollen polysaccharide composition contributed to the trade-off of these core bacteria.
36118209	2	17	theme	western	269:275	arg1	mellifera					291:299	the western honeybee Apis mellifera	265:299	the western honeybee Apis mellifera	265:299	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	7	18	theme	core	1387:1390	arg1	bacteria					1392:1399	these core bacteria	1381:1399	these core bacteria	1381:1399	By feeding and inoculation bioassays, we confirmed that the variations in pollen polysaccharide composition contributed to the trade-off of these core bacteria.
36118209	0	19	theme	compositional	12:24	arg1	variation					41:49	Significant compositional and functional variation	0:49	Significant compositional and functional variation	0:49	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	7	20	theme	inoculation	1256:1266	arg1	bioassays					1268:1276	feeding and inoculation bioassays	1244:1276	feeding and inoculation bioassays	1244:1276	By feeding and inoculation bioassays, we confirmed that the variations in pollen polysaccharide composition contributed to the trade-off of these core bacteria.
36118209	0	21	theme	gut	75:77	arg1	evolution					90:98	gut microbiota evolution	75:98	gut microbiota evolution	75:98	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	2	22	theme	honeybee	312:319	arg1	populations					334:344	the Asian honeybee, Apis cerana populations	302:344	populations	334:344	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	5	23	dep	composition	1034:1044	arg1	the					1030:1032	the	1030:1032	the	1030:1032	Pollen diets were significantly correlated with both the composition and function of the gut microbiome.
36118209	0	24	theme	Significant	0:10	arg1	variation					41:49	Significant compositional and functional variation	0:49	Significant compositional and functional variation	0:49	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	2	25	theme	Asian	306:310	arg1	populations					334:344	the Asian honeybee, Apis cerana populations	302:344	populations	334:344	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	9	26	from	polysaccharides	1646:1660	arg1	general					1626:1632	general	1626:1632	general	1626:1632	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	9	26	from	polysaccharides	1646:1660	arg1	particular					1665:1674	particular	1665:1674	particular	1665:1674	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	6	27	theme	Core	1082:1085	arg1	bacteria					1087:1094	Core bacteria	1082:1094	Core bacteria	1082:1094	Core bacteria, Gilliamella and Lactobacillus Firm-5, showed antagonistic turnovers and contributed to the enrichment in carbohydrate transport and metabolism.
36118209	6	27	theme	Core	1082:1085	arg1	Lactobacillus					1113:1125	Lactobacillus	1113:1125	Lactobacillus	1113:1125	Core bacteria, Gilliamella and Lactobacillus Firm-5, showed antagonistic turnovers and contributed to the enrichment in carbohydrate transport and metabolism.
36118209	6	27	theme	Core	1082:1085	arg1	Gilliamella					1097:1107	Gilliamella	1097:1107	Gilliamella	1097:1107	Core bacteria, Gilliamella and Lactobacillus Firm-5, showed antagonistic turnovers and contributed to the enrichment in carbohydrate transport and metabolism.
36118209	0	28	theme	functional	30:39	arg1	variation					41:49	Significant compositional and functional variation	0:49	Significant compositional and functional variation	0:49	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	8	29	theme	evolution	1476:1484	arg1	foundation					1447:1456	the foundation	1443:1456	the foundation of gut microbiome evolution among the Asian honeybee	1443:1509	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	8	29	theme	evolution	1476:1484	arg1	change					1414:1419	Progressive change	1402:1419	Progressive change	1402:1419	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	2	30	theme	geographic	550:559	arg1	scales					561:566	large geographic scales	544:566	large geographic scales	544:566	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	2	31	dep	populations	334:344	arg1	Apis					322:325	the Asian honeybee, Apis cerana populations	302:344	Apis	322:325	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	5	32	theme	Pollen	977:982	arg1	diets					984:988	Pollen diets	977:988	Pollen diets	977:988	Pollen diets were significantly correlated with both the composition and function of the gut microbiome.
36118209	2	33	theme	bee	421:423	arg1	management					425:434	bee management	421:434	bee management	421:434	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	0	34	theme	honeybee	127:134	arg1	populations					136:146	the widespread Asian honeybee populations	106:146	the widespread Asian honeybee populations	106:146	Significant compositional and functional variation reveals the patterns of gut microbiota evolution among the widespread Asian honeybee populations.
36118209	6	35	from	enrichment	1188:1197	arg1	metabolism					1229:1238	metabolism	1229:1238	metabolism	1229:1238	Core bacteria, Gilliamella and Lactobacillus Firm-5, showed antagonistic turnovers and contributed to the enrichment in carbohydrate transport and metabolism.
36118209	6	35	from	enrichment	1188:1197	arg1	transport					1215:1223	carbohydrate transport	1202:1223	carbohydrate transport	1202:1223	Core bacteria, Gilliamella and Lactobacillus Firm-5, showed antagonistic turnovers and contributed to the enrichment in carbohydrate transport and metabolism.
36118209	9	36	theme	gut	1563:1565	arg1	microbiomes					1567:1577	gut microbiomes	1563:1577	gut microbiomes	1563:1577	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	4	37	theme	Bacterial	711:719	arg1	composition					721:731	Bacterial composition	711:731	Bacterial composition	711:731	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	9	38	theme	pollen	1639:1644	arg1	polysaccharides					1646:1660	pollen polysaccharides	1639:1660	pollen polysaccharides in particular	1639:1674	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	8	39	dep	nestedness	1428:1437	arg1	i.e.					1422:1425	i.e.	1422:1425	i.e.	1422:1425	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	2	40	theme	good	454:457	arg1	system					459:464	a good system	452:464	a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales	452:566	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	3	41	theme	honeybees	626:634	arg1	microbiomes					602:612	the gut microbiomes	594:612	the gut microbiomes	594:612	We compared and analyzed the gut microbiomes of 99 Asian honeybees, from genetically diverged populations covering 13 provinces across China.
36118209	3	42	theme	gut	598:600	arg1	microbiomes					602:612	the gut microbiomes	594:612	the gut microbiomes	594:612	We compared and analyzed the gut microbiomes of 99 Asian honeybees, from genetically diverged populations covering 13 provinces across China.
36118209	4	43	theme	microbial	904:912	arg1	community					914:922	microbial community	904:922	microbial community	904:922	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	8	44	theme	Asian	1496:1500	arg1	honeybee					1502:1509	the Asian honeybee	1492:1509	the Asian honeybee	1492:1509	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	7	45	theme	pollen	1315:1320	arg1	composition					1337:1347	pollen polysaccharide composition	1315:1347	pollen polysaccharide composition	1315:1347	By feeding and inoculation bioassays, we confirmed that the variations in pollen polysaccharide composition contributed to the trade-off of these core bacteria.
36118209	4	46	theme	cerana	933:938	arg1	populations					940:950	A. cerana populations	930:950	A. cerana populations	930:950	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	2	47	theme	cerana	327:332	arg1	populations					334:344	the Asian honeybee, Apis cerana populations	302:344	populations	334:344	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	8	48	theme	microbiome	1465:1474	arg1	evolution					1476:1484	gut microbiome evolution	1461:1484	gut microbiome evolution	1461:1484	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	5	49	theme	gut	1066:1068	arg1	microbiome					1070:1079	the gut microbiome	1062:1079	the gut microbiome	1062:1079	Pollen diets were significantly correlated with both the composition and function of the gut microbiome.
36118209	8	50	theme	gut	1461:1463	arg1	evolution					1476:1484	gut microbiome evolution	1461:1484	gut microbiome evolution	1461:1484	Progressive change, i.e., nestedness, is the foundation of gut microbiome evolution among the Asian honeybee.
36118209	4	51	theme	strain	827:832	arg1	levels					834:839	phylotype, sequence-discrete population (SDP), and strain levels	776:839	phylotype, sequence-discrete population (SDP), and strain levels	776:839	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	6	52	theme	antagonistic	1142:1153	arg1	turnovers					1155:1163	antagonistic turnovers	1142:1163	antagonistic turnovers	1142:1163	Core bacteria, Gilliamella and Lactobacillus Firm-5, showed antagonistic turnovers and contributed to the enrichment in carbohydrate transport and metabolism.
36118209	4	53	theme	phylotype	776:784	arg1	levels					834:839	phylotype, sequence-discrete population (SDP), and strain levels	776:839	phylotype, sequence-discrete population (SDP), and strain levels	776:839	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	4	54	theme	population	805:814	arg1	levels					834:839	phylotype, sequence-discrete population (SDP), and strain levels	776:839	phylotype, sequence-discrete population (SDP), and strain levels	776:839	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	3	55	theme	diverged	654:661	arg1	populations					663:673	genetically diverged populations	642:673	genetically diverged populations covering 13 provinces across China	642:708	We compared and analyzed the gut microbiomes of 99 Asian honeybees, from genetically diverged populations covering 13 provinces across China.
36118209	3	56	theme	Asian	620:624	arg1	honeybees					626:634	99 Asian honeybees	617:634	99 Asian honeybees	617:634	We compared and analyzed the gut microbiomes of 99 Asian honeybees, from genetically diverged populations covering 13 provinces across China.
36118209	9	57	theme	microbiomes	1567:1577	arg1	co-diversification					1541:1558	the co-diversification	1537:1558	the co-diversification of gut microbiomes	1537:1577	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	9	58	from	diets	1617:1621	arg1	general					1626:1632	general	1626:1632	general	1626:1632	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	9	58	from	diets	1617:1621	arg1	particular					1665:1674	particular	1665:1674	particular	1665:1674	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	4	59	theme	A.	930:931	arg1	populations					940:950	A. cerana populations	930:950	A. cerana populations	930:950	Bacterial composition varied significantly across populations at phylotype, sequence-discrete population (SDP), and strain levels, but with extensive overlaps, indicating that the diversity of microbial community among A. cerana populations is driven by nestedness.
36118209	2	60	theme	gut	485:487	arg1	microbiota					489:498	gut microbiota	485:498	gut microbiota	485:498	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	7	61	theme	bacteria	1392:1399	arg1	trade-off					1368:1376	the trade-off	1364:1376	the trade-off of these core bacteria	1364:1399	By feeding and inoculation bioassays, we confirmed that the variations in pollen polysaccharide composition contributed to the trade-off of these core bacteria.
36118209	1	62	theme	gut	153:155	arg1	microbiome					157:166	The gut microbiome	149:166	The gut microbiome	149:166	The gut microbiome is a crucial element that facilitates a host's adaptation to a changing environment.
36118209	1	62	theme	gut	153:155	arg1	element					181:187	a crucial element	171:187	a crucial element that facilitates a host's adaptation to a changing environment	171:250	The gut microbiome is a crucial element that facilitates a host's adaptation to a changing environment.
36118209	6	63	theme	carbohydrate	1202:1213	arg1	transport					1215:1223	carbohydrate transport	1202:1223	carbohydrate transport	1202:1223	Core bacteria, Gilliamella and Lactobacillus Firm-5, showed antagonistic turnovers and contributed to the enrichment in carbohydrate transport and metabolism.
36118209	1	64	theme	changing	231:238	arg1	environment					240:250	a changing environment	229:250	a changing environment	229:250	The gut microbiome is a crucial element that facilitates a host's adaptation to a changing environment.
36118209	3	65	from	populations	663:673	arg1	microbiomes					602:612	the gut microbiomes	594:612	the gut microbiomes	594:612	We compared and analyzed the gut microbiomes of 99 Asian honeybees, from genetically diverged populations covering 13 provinces across China.
36118209	7	66	theme	polysaccharide	1322:1335	arg1	composition					1337:1347	pollen polysaccharide composition	1315:1347	pollen polysaccharide composition	1315:1347	By feeding and inoculation bioassays, we confirmed that the variations in pollen polysaccharide composition contributed to the trade-off of these core bacteria.
36118209	2	67	theme	natural	357:363	arg1	range					365:369	its natural range	353:369	its natural range	353:369	Compared to the western honeybee Apis mellifera, the Asian honeybee, Apis cerana populations across its natural range remain mostly semi-feral and are less affected by bee management, which provides a good system to investigate how gut microbiota evolve under environmental heterogeneity on large geographic scales.
36118209	9	68	from	factors	1608:1614	arg1	general					1626:1632	general	1626:1632	general	1626:1632	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36118209	9	68	from	factors	1608:1614	arg1	particular					1665:1674	particular	1665:1674	particular	1665:1674	Such a transition during the co-diversification of gut microbiomes is affected by environmental factors, diets in general, and pollen polysaccharides in particular.
36473370	0	0	theme	stem	83:86	arg1	cell					88:91	the EMT through targeted cancer stem cell	51:91	the EMT through targeted cancer stem cell	51:91	Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell.
36473370	2	1	theme	therapeutic	305:315	arg1	approaches					317:326	diagnostic and therapeutic approaches	290:326	diagnostic and therapeutic approaches for effective cancer treatment	290:357	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	2	1	theme	therapeutic	305:315	arg1	drugs					283:287	new drugs	279:287	new drugs	279:287	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	0	2	theme	cancer	76:81	arg1	cell					88:91	the EMT through targeted cancer stem cell	51:91	the EMT through targeted cancer stem cell	51:91	Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell.
36473370	5	3	theme	HA	798:799	arg1	CD44					792:795	CD44	792:795	CD44 (HA receptor)	792:809	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	5	3	theme	HA	798:799	arg1	receptor					801:808	HA receptor	798:808	HA receptor	798:808	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	1	4	theme	deadliest	139:147	arg1	tumors					149:154	the deadliest tumors	135:154	the deadliest tumors in the world with a high rate of recurrence and metastasis	135:213	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	6	5	theme	stemness-related	881:896	arg1	OCT4					904:907	OCT4	904:907	OCT4	904:907	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	6	5	theme	stemness-related	881:896	arg1	genes					898:902	stemness-related genes	881:902	stemness-related genes OCT4 and Nanog	881:917	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	6	5	theme	stemness-related	881:896	arg1	Nanog					913:917	Nanog	913:917	Nanog	913:917	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	7	6	dep	markers	1098:1104	arg1	markers					1098:1104	epithelial-mesenchymal transition (EMT) process markers	1050:1104	epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin	1050:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	6	dep	markers	1098:1104	arg1	Vimentin					1118:1125	Vimentin	1118:1125	Vimentin	1118:1125	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	6	dep	markers	1098:1104	arg1	N-cadherin					1132:1141	N-cadherin	1132:1141	N-cadherin	1132:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	6	dep	markers	1098:1104	arg1	E-cadherin					1106:1115	E-cadherin	1106:1115	E-cadherin	1106:1115	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	8	7	theme	Dual	1144:1147	arg1	targeting					1149:1157	Dual targeting	1144:1157	Dual targeting using magnetic and receptor-mediated targeting	1144:1204	Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system.
36473370	3	8	theme	Cancer	360:365	arg1	CSCs					379:382	CSCs	379:382	CSCs	379:382	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	3	8	theme	Cancer	360:365	arg1	cells					372:376	Cancer stem cells	360:376	Cancer stem cells (CSCs)	360:383	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	4	9	theme	hyaluronic	683:692	arg1	acid					694:697	hyaluronic acid	683:697	hyaluronic acid (HA)	683:702	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	4	9	theme	hyaluronic	683:692	arg1	HA					700:701	HA	700:701	HA	700:701	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	4	10	theme	magnetic	624:631	arg1	nanocubes					633:641	composite magnetic nanocubes	614:641	composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA)	614:702	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	4	11	theme	thermal	568:574	arg1	approach					586:593	a high-temperature thermal breakdown approach	549:593	a high-temperature thermal breakdown approach	549:593	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	3	12	theme	tumor	426:430	arg1	resistance					432:441	tumor resistance	426:441	tumor resistance	426:441	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	2	13	theme	pressing	236:243	arg1	issue					245:249	the most pressing issue	227:249	the most pressing issue	227:249	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	2	13	theme	pressing	236:243	arg1	development					264:274	the development	260:274	the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment	260:357	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	5	14	theme	HA	785:786	arg1	CD44					792:795	CD44	792:795	CD44 (HA receptor)	792:809	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	5	14	theme	HA	785:786	arg1	receptor					801:808	HA receptor	798:808	HA receptor	798:808	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	5	14	theme	HA	785:786	arg1	binding					774:780	receptor-ligand binding	758:780	receptor-ligand binding of HA	758:786	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	1	15	theme	tumors	149:154	arg1	tumors					149:154	the deadliest tumors	135:154	the deadliest tumors in the world with a high rate of recurrence and metastasis	135:213	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	1	15	theme	tumors	149:154	arg1	one					128:130	one	128:130	one	128:130	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	5	16	theme	Fe3O4	709:713	arg1	nanocubes					715:723	The Fe3O4 nanocubes	705:723	The Fe3O4 nanocubes	705:723	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	7	17	theme	epithelial-mesenchymal	1050:1071	arg1	EMT					1085:1087	EMT	1085:1087	EMT	1085:1087	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	17	theme	epithelial-mesenchymal	1050:1071	arg1	transition					1073:1082	epithelial-mesenchymal transition	1050:1082	epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin	1050:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	9	18	theme	potential	1321:1329	arg1	method					1303:1308	the medication delivery method	1279:1308	the medication delivery method	1279:1308	Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
36473370	9	18	theme	potential	1321:1329	arg1	strategy					1343:1350	a potential therapeutic strategy	1319:1350	a potential therapeutic strategy for HCC	1319:1358	Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
36473370	4	19	theme	breakdown	576:584	arg1	approach					586:593	a high-temperature thermal breakdown approach	549:593	a high-temperature thermal breakdown approach	549:593	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	7	20	theme	markers	1098:1104	arg1	expression					1036:1045	the expression	1032:1045	the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin	1032:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	2	21	theme	cancer	342:347	arg1	treatment					349:357	effective cancer treatment	332:357	effective cancer treatment	332:357	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	0	22	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell.
36473370	2	23	theme	effective	332:340	arg1	treatment					349:357	effective cancer treatment	332:357	effective cancer treatment	332:357	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	0	24	theme	Fe3O4	25:29	arg1	nanocubes					31:39	Fe3O4 nanocubes	25:39	Fe3O4 nanocubes	25:39	Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell.
36473370	8	25	theme	delivery	1238:1245	arg1	system					1247:1252	the drug delivery system	1229:1252	the drug delivery system	1229:1252	Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system.
36473370	7	26	theme	HCC	981:983	arg1	proliferation					990:1002	HCC cell proliferation	981:1002	HCC cell proliferation	981:1002	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	1	27	theme	Hepatocellular	94:107	arg1	HCC					120:122	HCC	120:122	HCC	120:122	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	1	27	theme	Hepatocellular	94:107	arg1	carcinoma					109:117	Hepatocellular carcinoma	94:117	Hepatocellular carcinoma (HCC)	94:123	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	5	28	theme	stem	743:746	arg1	cells					748:752	HCC stem cells	739:752	HCC stem cells	739:752	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	5	29	theme	HCC	739:741	arg1	cells					748:752	HCC stem cells	739:752	HCC stem cells	739:752	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	7	30	theme	cell	985:988	arg1	proliferation					990:1002	HCC cell proliferation	981:1002	HCC cell proliferation	981:1002	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	4	31	theme	composite	614:622	arg1	nanocubes					633:641	composite magnetic nanocubes	614:641	composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA)	614:702	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	3	32	theme	tumor	408:412	arg1	recurrence					414:423	tumor recurrence	408:423	tumor recurrence	408:423	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	9	33	theme	medication	1283:1292	arg1	method					1303:1308	the medication delivery method	1279:1308	the medication delivery method	1279:1308	Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
36473370	9	33	theme	medication	1283:1292	arg1	strategy					1343:1350	a potential therapeutic strategy	1319:1350	a potential therapeutic strategy for HCC	1319:1358	Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
36473370	3	34	theme	pivotal	392:398	arg1	role					400:403	a pivotal role	390:403	a pivotal role	390:403	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	3	35	theme	liver	521:525	arg1	cancer					527:532	liver cancer	521:532	liver cancer	521:532	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	1	36	theme	high	176:179	arg1	rate					181:184	a high rate	174:184	a high rate of recurrence and metastasis	174:213	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	8	37	theme	magnetic	1165:1172	arg1	targeting					1196:1204	magnetic and receptor-mediated targeting	1165:1204	magnetic and receptor-mediated targeting	1165:1204	Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system.
36473370	9	38	theme	delivery	1294:1301	arg1	method					1303:1308	the medication delivery method	1279:1308	the medication delivery method	1279:1308	Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
36473370	9	38	theme	delivery	1294:1301	arg1	strategy					1343:1350	a potential therapeutic strategy	1319:1350	a potential therapeutic strategy for HCC	1319:1358	Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
36473370	3	39	theme	cancer	527:532	arg1	development					506:516	the development	502:516	the development of liver cancer	502:532	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	8	40	theme	drug	1233:1236	arg1	system					1247:1252	the drug delivery system	1229:1252	the drug delivery system	1229:1252	Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system.
36473370	4	41	theme	high-temperature	551:566	arg1	approach					586:593	a high-temperature thermal breakdown approach	549:593	a high-temperature thermal breakdown approach	549:593	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	0	42	theme	EMT	55:57	arg1	cell					88:91	the EMT through targeted cancer stem cell	51:91	the EMT through targeted cancer stem cell	51:91	Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell.
36473370	6	43	theme	molecule	834:841	arg1	LDN193189					843:851	a small molecule LDN193189	826:851	a small molecule LDN193189	826:851	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	7	44	theme	Fe3O4	940:944	arg1	nanocubes					946:954	the Fe3O4 nanocubes	936:954	the Fe3O4 nanocubes	936:954	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	9	45	dep	potential	1321:1329	arg1	therapeutic					1331:1341	therapeutic	1331:1341	therapeutic	1331:1341	Our findings imply that the medication delivery method might be a potential therapeutic strategy for HCC.
36473370	4	46	used	used	599:602	arg2	approach					586:593	a high-temperature thermal breakdown approach	549:593	a high-temperature thermal breakdown approach	549:593	In the study, a high-temperature thermal breakdown approach was used to create composite magnetic nanocubes modified by polyethyleneimine (PEI) and hyaluronic acid (HA).
36473370	2	47	theme	diagnostic	290:299	arg1	approaches					317:326	diagnostic and therapeutic approaches	290:326	diagnostic and therapeutic approaches for effective cancer treatment	290:357	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	2	47	theme	diagnostic	290:299	arg1	drugs					283:287	new drugs	279:287	new drugs	279:287	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	3	48	theme	stem	367:370	arg1	CSCs					379:382	CSCs	379:382	CSCs	379:382	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	3	48	theme	stem	367:370	arg1	cells					372:376	Cancer stem cells	360:376	Cancer stem cells (CSCs)	360:383	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	6	49	theme	small	828:832	arg1	LDN193189					843:851	a small molecule LDN193189	826:851	a small molecule LDN193189	826:851	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	1	50	with	tumors	149:154	arg1	rate					181:184	a high rate	174:184	a high rate of recurrence and metastasis	174:213	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	7	51	theme	transition	1073:1082	arg1	markers					1098:1104	epithelial-mesenchymal transition (EMT) process markers	1050:1104	epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin	1050:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	51	theme	transition	1073:1082	arg1	Vimentin					1118:1125	Vimentin	1118:1125	Vimentin	1118:1125	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	51	theme	transition	1073:1082	arg1	N-cadherin					1132:1141	N-cadherin	1132:1141	N-cadherin	1132:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	51	theme	transition	1073:1082	arg1	E-cadherin					1106:1115	E-cadherin	1106:1115	E-cadherin	1106:1115	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	8	52	theme	receptor-mediated	1178:1194	arg1	targeting					1196:1204	magnetic and receptor-mediated targeting	1165:1204	magnetic and receptor-mediated targeting	1165:1204	Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system.
36473370	2	53	theme	drugs	283:287	arg1	issue					245:249	the most pressing issue	227:249	the most pressing issue	227:249	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	2	53	theme	drugs	283:287	arg1	development					264:274	the development	260:274	the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment	260:357	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	3	54	theme	new	483:485	arg1	perspective					487:497	a new perspective	481:497	a new perspective on the development of liver cancer	481:532	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	6	55	theme	genes	898:902	arg1	expression					867:876	the expression	863:876	the expression of stemness-related genes OCT4 and Nanog	863:917	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	2	56	theme	new	279:281	arg1	approaches					317:326	diagnostic and therapeutic approaches	290:326	diagnostic and therapeutic approaches for effective cancer treatment	290:357	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	2	56	theme	new	279:281	arg1	drugs					283:287	new drugs	279:287	new drugs	279:287	Therefore, the most pressing issue today is the development of new drugs, diagnostic and therapeutic approaches for effective cancer treatment.
36473370	1	57	theme	recurrence	189:198	arg1	rate					181:184	a high rate	174:184	a high rate of recurrence and metastasis	174:213	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	0	58	theme	targeted	67:74	arg1	cell					88:91	the EMT through targeted cancer stem cell	51:91	the EMT through targeted cancer stem cell	51:91	Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell.
36473370	5	59	theme	receptor-ligand	758:772	arg1	binding					774:780	receptor-ligand binding	758:780	receptor-ligand binding of HA	758:786	The Fe3O4 nanocubes can recognize HCC stem cells via receptor-ligand binding of HA and CD44 (HA receptor).
36473370	3	60	theme	tumor	448:452	arg1	metastasis					454:463	tumor metastasis	448:463	tumor metastasis	448:463	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	3	61	from	perspective	487:497	arg1	development					506:516	the development	502:516	the development of liver cancer	502:532	Cancer stem cells (CSCs) play a pivotal role in tumor recurrence, tumor resistance, and tumor metastasis, which provides a new perspective on the development of liver cancer.
36473370	0	62	theme	through	59:65	arg1	cell					88:91	the EMT through targeted cancer stem cell	51:91	the EMT through targeted cancer stem cell	51:91	Hyaluronic acid mediated Fe3O4 nanocubes reversing the EMT through targeted cancer stem cell.
36473370	6	63	dep	genes	898:902	arg1	OCT4					904:907	OCT4	904:907	OCT4	904:907	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	6	63	dep	genes	898:902	arg1	genes					898:902	stemness-related genes	881:902	stemness-related genes OCT4 and Nanog	881:917	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	6	63	dep	genes	898:902	arg1	Nanog					913:917	Nanog	913:917	Nanog	913:917	While loading a small molecule LDN193189 inhibited the expression of stemness-related genes OCT4 and Nanog.
36473370	7	64	theme	process	1090:1096	arg1	markers					1098:1104	epithelial-mesenchymal transition (EMT) process markers	1050:1104	epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin	1050:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	64	theme	process	1090:1096	arg1	Vimentin					1118:1125	Vimentin	1118:1125	Vimentin	1118:1125	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	64	theme	process	1090:1096	arg1	N-cadherin					1132:1141	N-cadherin	1132:1141	N-cadherin	1132:1141	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	7	64	theme	process	1090:1096	arg1	E-cadherin					1106:1115	E-cadherin	1106:1115	E-cadherin	1106:1115	More crucially, the Fe3O4 nanocubes significantly suppressed HCC cell proliferation and migration by regulating the expression of epithelial-mesenchymal transition (EMT) process markers E-cadherin, Vimentin, and N-cadherin.
36473370	1	65	from	tumors	149:154	arg1	world					163:167	the world	159:167	the world	159:167	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
36473370	8	66	theme	system	1247:1252	arg1	uptake					1219:1224	the uptake	1215:1224	the uptake of the drug delivery system	1215:1252	Dual targeting using magnetic and receptor-mediated targeting improved the uptake of the drug delivery system.
36473370	1	67	theme	metastasis	204:213	arg1	rate					181:184	a high rate	174:184	a high rate of recurrence and metastasis	174:213	Hepatocellular carcinoma (HCC) is one of the deadliest tumors in the world with a high rate of recurrence and metastasis.
35409181	9	0	from	chromosomes	1021:1031	arg1	formation					1058:1066	the formation	1054:1066	the formation of grain β-glucan content	1054:1092	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	5	1	theme	edible	617:622	arg1	content					630:636	edible fiber content	617:636	edible fiber content	617:636	A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae.
35409181	1	2	theme	bread	147:151	arg1	wheat					153:157	bread wheat	147:157	bread wheat	147:157	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	10	3	theme	genes	1302:1306	arg1	nine					1309:1312	nine	1309:1312	nine	1309:1312	Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots.
35409181	10	3	theme	genes	1302:1306	arg1	genes					1302:1306	fourteen genes	1293:1306	fourteen genes	1293:1306	Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots.
35409181	9	4	from	role	1004:1007	arg1	chromosomes					1021:1031	chromosomes 1Mb, 4Mb, and 5Mb	1021:1049	chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content	1021:1092	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	4	from	role	1004:1007	arg1	4Mb					1038:1040	4Mb	1038:1040	4Mb	1038:1040	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	4	from	role	1004:1007	arg1	5Mb					1047:1049	5Mb	1047:1049	5Mb	1047:1049	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	4	from	role	1004:1007	arg1	1Mb					1033:1035	1Mb	1033:1035	1Mb	1033:1035	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	5	5	theme	fiber	624:628	arg1	content					630:636	edible fiber content	617:636	edible fiber content	617:636	A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae.
35409181	1	6	theme	wheat	153:157	arg1	trait					138:142	an important health-promoting trait	108:142	an important health-promoting trait of bread wheat	108:157	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	1	6	theme	wheat	153:157	arg1	content					97:103	Grain dietary fiber content	77:103	Grain dietary fiber content	77:103	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	10	7	theme	Functional	1225:1234	arg1	annotation					1236:1245	Functional annotation	1225:1245	Functional annotation of the associated marker sequences	1225:1280	Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots.
35409181	11	8	theme	gene	1455:1458	arg1	transfer					1460:1467	chromosome-mediated gene transfer	1435:1467	chromosome-mediated gene transfer	1435:1467	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	2	9	with	lines	372:376	arg1	composition					398:408	modified fiber composition	383:408	modified fiber composition	383:408	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	10	theme	attractive	332:341	arg1	source					348:353	an attractive gene source	329:353	an attractive gene source to develop wheat lines with modified fiber composition	329:408	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	9	11	dep	chromosomes	1021:1031	arg1	chromosomes					1021:1031	chromosomes 1Mb, 4Mb, and 5Mb	1021:1049	chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content	1021:1092	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	11	dep	chromosomes	1021:1031	arg1	4Mb					1038:1040	4Mb	1038:1040	4Mb	1038:1040	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	11	dep	chromosomes	1021:1031	arg1	5Mb					1047:1049	5Mb	1047:1049	5Mb	1047:1049	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	11	dep	chromosomes	1021:1031	arg1	1Mb					1033:1035	1Mb	1033:1035	1Mb	1033:1035	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	7	12	theme	protein	880:886	arg1	content					888:894	protein content	880:894	protein content	880:894	Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content.
35409181	8	13	theme	progenitors	959:969	arg1	assemblies					937:946	draft chromosome assemblies	920:946	draft chromosome assemblies of diploid progenitors of Ae	920:975	Mapping the markers to draft chromosome assemblies of diploid progenitors of Ae.
35409181	0	14	from	Content	45:51	arg1	biuncialis					65:74	Aegilops biuncialis	56:74	Aegilops biuncialis	56:74	Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.
35409181	3	15	theme	protein	527:533	arg1	content					535:541	protein content	527:541	protein content	527:541	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	5	16	theme	large	580:584	arg1	variation					586:594	A large variation	578:594	A large variation in grain protein and edible fiber content	578:636	A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae.
35409181	2	17	theme	gene	343:346	arg1	source					348:353	an attractive gene source	329:353	an attractive gene source to develop wheat lines with modified fiber composition	329:408	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	10	18	theme	sequences	1272:1280	arg1	annotation					1236:1245	Functional annotation	1225:1245	Functional annotation of the associated marker sequences	1225:1280	Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots.
35409181	9	19	theme	grain	1071:1075	arg1	content					1086:1092	grain β-glucan content	1071:1092	grain β-glucan content	1071:1092	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	6	20	from	habitats	735:742	arg1	accessions					692:701	accessions	692:701	accessions	692:701	biuncialis accessions from different eco-geographical habitats.
35409181	11	21	theme	health-promoting	1484:1499	arg1	properties					1501:1510	the health-promoting properties	1480:1510	the health-promoting properties of wheat-derived foods	1480:1533	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	11	22	theme	wheat-derived	1515:1527	arg1	foods					1529:1533	wheat-derived foods	1515:1533	wheat-derived foods	1515:1533	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	2	23	theme	Aegilops	267:274	arg1	biuncialis					276:285	the goatgrass Aegilops biuncialis	253:285	the goatgrass Aegilops biuncialis	253:285	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	10	24	theme	marker	1265:1270	arg1	sequences					1272:1280	the associated marker sequences	1250:1280	the associated marker sequences	1250:1280	Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots.
35409181	5	25	theme	grain	599:603	arg1	protein					605:611	grain protein	599:611	grain protein	599:611	A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae.
35409181	2	26	theme	goatgrass	257:265	arg1	biuncialis					276:285	the goatgrass Aegilops biuncialis	253:285	the goatgrass Aegilops biuncialis	253:285	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	10	27	theme	associated	1254:1263	arg1	sequences					1272:1280	the associated marker sequences	1250:1280	the associated marker sequences	1250:1280	Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots.
35409181	11	28	theme	attractive	1412:1421	arg1	QTLs					1362:1365	QTLs	1362:1365	QTLs	1362:1365	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	11	28	theme	attractive	1412:1421	arg1	genes					1371:1375	genes	1371:1375	genes	1371:1375	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	11	28	theme	attractive	1412:1421	arg1	targets					1423:1429	attractive targets	1412:1429	attractive targets for chromosome-mediated gene transfer	1412:1467	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	7	29	theme	34	804:805	arg1	QTLs					807:810	34 QTLs	804:810	34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content	804:894	Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content.
35409181	9	30	theme	responsible	1162:1172	arg1	genes					1156:1160	genes	1156:1160	genes responsible for total- and water-extractable pentosan content	1156:1222	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	1	31	theme	Grain	77:81	arg1	trait					138:142	an important health-promoting trait	108:142	an important health-promoting trait of bread wheat	108:157	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	1	31	theme	Grain	77:81	arg1	content					97:103	Grain dietary fiber content	77:103	Grain dietary fiber content	77:103	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	7	32	theme	water-extractable	848:864	arg1	pentosan					866:873	water-extractable pentosan	848:873	water-extractable pentosan	848:873	Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content.
35409181	2	33	theme	wheat	366:370	arg1	lines					372:376	wheat lines	366:376	wheat lines with modified fiber composition	366:408	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	11	34	dep	QTLs	1362:1365	arg1	The					1358:1360	The	1358:1360	The	1358:1360	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	10	35	theme	other	1342:1346	arg1	monocots					1348:1355	other monocots	1342:1355	other monocots	1342:1355	Functional annotation of the associated marker sequences identified fourteen genes, nine of which were identified in other monocots.
35409181	1	36	theme	dietary	83:89	arg1	trait					138:142	an important health-promoting trait	108:142	an important health-promoting trait of bread wheat	108:157	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	1	36	theme	dietary	83:89	arg1	content					97:103	Grain dietary fiber content	77:103	Grain dietary fiber content	77:103	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	9	37	theme	β-glucan	1077:1084	arg1	content					1086:1092	grain β-glucan content	1071:1092	grain β-glucan content	1071:1092	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	38	theme	water-extractable	1189:1205	arg1	content					1216:1222	water-extractable pentosan content	1189:1222	water-extractable pentosan content	1189:1222	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	0	39	theme	QTLs	22:25	arg1	Identification					0:13	Identification	0:13	Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.	0:75	Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.
35409181	3	40	from	variability	482:492	arg1	pentosan					513:520	pentosan	513:520	pentosan	513:520	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	3	40	from	variability	482:492	arg1	β-glucan					503:510	grain β-glucan	497:510	grain β-glucan	497:510	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	3	40	from	variability	482:492	arg1	content					535:541	protein content	527:541	protein content	527:541	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	3	40	from	variability	482:492	arg1	collection					548:557	a collection	546:557	a collection of Ae	546:563	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	2	41	theme	β-glucan	296:303	arg1	content					305:311	high β-glucan content	291:311	high β-glucan content	291:311	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	9	42	theme	content	1086:1092	arg1	formation					1058:1066	the formation	1054:1066	the formation of grain β-glucan content	1054:1092	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	0	43	theme	New	18:20	arg1	QTLs					22:25	New QTLs	18:25	New QTLs for Dietary Fiber Content in Aegilops biuncialis	18:74	Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.
35409181	7	44	link	DArTseq-derived	772:786	arg1	SNPs					788:791	DArTseq-derived SNPs	772:791	DArTseq-derived SNPs	772:791	Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content.
35409181	2	45	theme	dietary	171:177	arg1	component					185:193	A dominant dietary fiber component	160:193	A dominant dietary fiber component of wheat	160:202	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	45	theme	dietary	171:177	arg1	arabinoxylan					236:247	the cell wall polysaccharide arabinoxylan	207:247	the cell wall polysaccharide arabinoxylan	207:247	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	46	theme	high	291:294	arg1	content					305:311	high β-glucan content	291:311	high β-glucan content	291:311	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	11	47	theme	chromosome-mediated	1435:1453	arg1	transfer					1460:1467	chromosome-mediated gene transfer	1435:1467	chromosome-mediated gene transfer	1435:1467	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	0	48	theme	Dietary	31:37	arg1	Content					45:51	Dietary Fiber Content	31:51	Dietary Fiber Content in Aegilops biuncialis	31:74	Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.
35409181	2	49	contain	has	287:289	arg1	biuncialis					276:285	the goatgrass Aegilops biuncialis	253:285	the goatgrass Aegilops biuncialis	253:285	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	49	contain	has	287:289	arg2	content					305:311	high β-glucan content	291:311	high β-glucan content	291:311	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	5	50	theme	Ae	677:678	arg1	origin					667:672	the origin	663:672	the origin of Ae	663:678	A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae.
35409181	2	51	theme	dominant	162:169	arg1	component					185:193	A dominant dietary fiber component	160:193	A dominant dietary fiber component of wheat	160:202	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	51	theme	dominant	162:169	arg1	arabinoxylan					236:247	the cell wall polysaccharide arabinoxylan	207:247	the cell wall polysaccharide arabinoxylan	207:247	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	52	theme	wall	216:219	arg1	arabinoxylan					236:247	the cell wall polysaccharide arabinoxylan	207:247	the cell wall polysaccharide arabinoxylan	207:247	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	52	theme	wall	216:219	arg1	component					185:193	A dominant dietary fiber component	160:193	A dominant dietary fiber component of wheat	160:202	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	53	theme	fiber	392:396	arg1	composition					398:408	modified fiber composition	383:408	modified fiber composition	383:408	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	7	54	theme	DArTseq-derived	772:786	arg1	SNPs					788:791	DArTseq-derived SNPs	772:791	DArTseq-derived SNPs	772:791	Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content.
35409181	9	55	theme	pentosan	1207:1214	arg1	content					1216:1222	water-extractable pentosan content	1189:1222	water-extractable pentosan content	1189:1222	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	2	56	theme	cell	211:214	arg1	arabinoxylan					236:247	the cell wall polysaccharide arabinoxylan	207:247	the cell wall polysaccharide arabinoxylan	207:247	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	56	theme	cell	211:214	arg1	component					185:193	A dominant dietary fiber component	160:193	A dominant dietary fiber component of wheat	160:202	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	8	57	theme	chromosome	926:935	arg1	assemblies					937:946	draft chromosome assemblies	920:946	draft chromosome assemblies of diploid progenitors of Ae	920:975	Mapping the markers to draft chromosome assemblies of diploid progenitors of Ae.
35409181	2	58	theme	modified	383:390	arg1	composition					398:408	modified fiber composition	383:408	modified fiber composition	383:408	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	9	59	theme	genes	1012:1016	arg1	role					1004:1007	the role	1000:1007	the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content	1000:1092	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	60	from	QTLs	1107:1110	arg1	groups					1126:1131	chromosome groups	1115:1131	chromosome groups 3, 6, and 1	1115:1143	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	8	61	theme	draft	920:924	arg1	assemblies					937:946	draft chromosome assemblies	920:946	draft chromosome assemblies of diploid progenitors of Ae	920:975	Mapping the markers to draft chromosome assemblies of diploid progenitors of Ae.
35409181	3	62	theme	genetic	474:480	arg1	variability					482:492	genetic variability	474:492	genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae	474:563	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	0	63	theme	Fiber	39:43	arg1	Content					45:51	Dietary Fiber Content	31:51	Dietary Fiber Content in Aegilops biuncialis	31:74	Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.
35409181	11	64	theme	present	1395:1401	arg1	work					1403:1406	the present work	1391:1406	the present work	1391:1406	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	2	65	theme	wheat	198:202	arg1	component					185:193	A dominant dietary fiber component	160:193	A dominant dietary fiber component of wheat	160:202	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	65	theme	wheat	198:202	arg1	arabinoxylan					236:247	the cell wall polysaccharide arabinoxylan	207:247	the cell wall polysaccharide arabinoxylan	207:247	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	3	66	theme	Ae	562:563	arg1	collection					548:557	a collection	546:557	a collection of Ae	546:563	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	3	67	theme	grain	497:501	arg1	β-glucan					503:510	grain β-glucan	497:510	grain β-glucan	497:510	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	11	68	link	wheat-derived	1515:1527	arg1	foods					1529:1533	wheat-derived foods	1515:1533	wheat-derived foods	1515:1533	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	11	69	theme	foods	1529:1533	arg1	properties					1501:1510	the health-promoting properties	1480:1510	the health-promoting properties of wheat-derived foods	1480:1533	The QTLs and genes identified in the present work are attractive targets for chromosome-mediated gene transfer to improve the health-promoting properties of wheat-derived foods.
35409181	6	70	theme	eco-geographical	718:733	arg1	habitats					735:742	different eco-geographical habitats	708:742	different eco-geographical habitats	708:742	biuncialis accessions from different eco-geographical habitats.
35409181	1	71	theme	important	111:119	arg1	trait					138:142	an important health-promoting trait	108:142	an important health-promoting trait of bread wheat	108:157	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	1	71	theme	important	111:119	arg1	content					97:103	Grain dietary fiber content	77:103	Grain dietary fiber content	77:103	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	1	72	theme	fiber	91:95	arg1	trait					138:142	an important health-promoting trait	108:142	an important health-promoting trait of bread wheat	108:157	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	1	72	theme	fiber	91:95	arg1	content					97:103	Grain dietary fiber content	77:103	Grain dietary fiber content	77:103	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	9	73	theme	chromosome	1115:1124	arg1	groups					1126:1131	chromosome groups	1115:1131	chromosome groups 3, 6, and 1	1115:1143	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	9	74	theme	other	1101:1105	arg1	QTLs					1107:1110	other QTLs	1101:1110	other QTLs on chromosome groups 3, 6, and 1	1101:1143	biuncialis underlined the role of genes on chromosomes 1Mb, 4Mb, and 5Mb in the formation of grain β-glucan content, while other QTLs on chromosome groups 3, 6, and 1 identified genes responsible for total- and water-extractable pentosan content.
35409181	6	75	theme	different	708:716	arg1	habitats					735:742	different eco-geographical habitats	708:742	different eco-geographical habitats	708:742	biuncialis accessions from different eco-geographical habitats.
35409181	1	76	theme	health-promoting	121:136	arg1	trait					138:142	an important health-promoting trait	108:142	an important health-promoting trait of bread wheat	108:157	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	1	76	theme	health-promoting	121:136	arg1	content					97:103	Grain dietary fiber content	77:103	Grain dietary fiber content	77:103	Grain dietary fiber content is an important health-promoting trait of bread wheat.
35409181	7	77	theme	Association	745:755	arg1	analysis					757:764	Association analysis	745:764	Association analysis using DArTseq-derived SNPs	745:791	Association analysis using DArTseq-derived SNPs identified 34 QTLs associated with β-glucan, pentosan, water-extractable pentosan, and protein content.
35409181	0	78	theme	Aegilops	56:63	arg1	biuncialis					65:74	Aegilops biuncialis	56:74	Aegilops biuncialis	56:74	Identification of New QTLs for Dietary Fiber Content in Aegilops biuncialis.
35409181	2	79	theme	fiber	179:183	arg1	component					185:193	A dominant dietary fiber component	160:193	A dominant dietary fiber component of wheat	160:202	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	79	theme	fiber	179:183	arg1	arabinoxylan					236:247	the cell wall polysaccharide arabinoxylan	207:247	the cell wall polysaccharide arabinoxylan	207:247	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	3	80	theme	introgression	431:443	arg1	breeding					445:452	introgression breeding	431:452	introgression breeding	431:452	In order to support introgression breeding, this work examined genetic variability in grain β-glucan, pentosan, and protein content in a collection of Ae.
35409181	8	81	theme	diploid	951:957	arg1	progenitors					959:969	diploid progenitors	951:969	diploid progenitors of Ae	951:975	Mapping the markers to draft chromosome assemblies of diploid progenitors of Ae.
35409181	2	82	theme	polysaccharide	221:234	arg1	arabinoxylan					236:247	the cell wall polysaccharide arabinoxylan	207:247	the cell wall polysaccharide arabinoxylan	207:247	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	2	82	theme	polysaccharide	221:234	arg1	component					185:193	A dominant dietary fiber component	160:193	A dominant dietary fiber component of wheat	160:202	A dominant dietary fiber component of wheat is the cell wall polysaccharide arabinoxylan and the goatgrass Aegilops biuncialis has high β-glucan content, which makes it an attractive gene source to develop wheat lines with modified fiber composition.
35409181	8	83	theme	Ae	974:975	arg1	progenitors					959:969	diploid progenitors	951:969	diploid progenitors of Ae	951:975	Mapping the markers to draft chromosome assemblies of diploid progenitors of Ae.
35409181	5	84	from	variation	586:594	arg1	protein					605:611	grain protein	599:611	grain protein	599:611	A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae.
35409181	5	84	from	variation	586:594	arg1	content					630:636	edible fiber content	617:636	edible fiber content	617:636	A large variation in grain protein and edible fiber content was revealed, reflecting the origin of Ae.
35932203	6	0	theme	%	999:999	arg1	extract					984:990	ether extract	978:990	ether extract (EE) (3%-7%)	978:1003	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	0	theme	%	999:999	arg1	%					1002:1002	3%-7%	998:1002	3%-7%	998:1002	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	2	1	from	intake	368:373	arg1	cows					494:497	lactating dairy cows	478:497	lactating dairy cows	478:497	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	2	theme	matter	427:432	arg1	coefficient					402:412	apparent digestibility coefficient	379:412	apparent digestibility coefficient (ADC)	379:418	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	2	theme	matter	427:432	arg1	production					448:457	milk production	443:457	milk production	443:457	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	2	theme	matter	427:432	arg1	composition					463:473	composition	463:473	composition	463:473	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	2	theme	matter	427:432	arg1	intake					368:373	intake	368:373	intake	368:373	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	2	theme	matter	427:432	arg1	ADC					415:417	ADC	415:417	ADC	415:417	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	3	theme	component	309:317	arg1	analysis					319:326	principal component analysis	299:326	principal component analysis	299:326	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	4	4	theme	weight	795:800	arg1	function					765:772	a function	763:772	a function of the animal's body weight	763:800	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
35932203	2	5	from	coefficient	402:412	arg1	cows					494:497	lactating dairy cows	478:497	lactating dairy cows	478:497	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	0	6	dep	fed	90:92	arg1	meta-analysis					133:145	A meta-analysis	131:145	A meta-analysis	131:145	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	3	7	with	studies	615:621	arg1	types					631:635	18 types	628:635	18 types of cakes evaluated in 1350 cows	628:667	The data set used in the meta-analysis came from 51 studies published between 2009 and 2019, which resulted in 119 studies with 18 types of cakes evaluated in 1350 cows.
35932203	0	8	theme	composition	70:80	arg1	Intake					0:5	Intake	0:5	Intake	0:5	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	0	8	theme	composition	70:80	arg1	digestibility					20:32	apparent digestibility	11:32	apparent digestibility	11:32	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	7	9	theme	milk	1139:1142	arg1	production					1144:1153	milk production	1139:1153	milk production	1139:1153	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	1	10	theme	animal	228:233	arg1	performance					235:245	animal performance	228:245	animal performance	228:245	Oilseed cakes can partially replace corn or soy used in the diet without losing animal performance.
35932203	2	11	theme	milk	443:446	arg1	production					448:457	milk production	443:457	milk production	443:457	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	6	12	theme	detergent	944:952	arg1	NDF					961:963	NDF	961:963	NDF	961:963	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	12	theme	detergent	944:952	arg1	fiber					954:958	neutral detergent fiber	936:958	neutral detergent fiber (NDF) (26%-%)	936:972	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	7	13	theme	milk	1250:1253	arg1	content					1263:1269	the milk protein content	1246:1269	the milk protein content of milk	1246:1277	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	2	14	theme	cakes	355:359	arg1	effects					344:350	the effects	340:350	the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows	340:497	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	0	15	theme	cows	85:88	arg1	matter					41:46	dry matter	37:46	dry matter	37:46	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	0	15	theme	cows	85:88	arg1	production					54:63	milk production	49:63	milk production	49:63	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	0	15	theme	cows	85:88	arg1	composition					70:80	composition	70:80	composition of cows	70:88	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	0	16	theme	dry	37:39	arg1	matter					41:46	dry matter	37:46	dry matter	37:46	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	1	17	theme	Oilseed	148:154	arg1	cakes					156:160	Oilseed cakes	148:160	Oilseed cakes	148:160	Oilseed cakes can partially replace corn or soy used in the diet without losing animal performance.
35932203	4	18	theme	0.366 kg	727:734	arg1	P < 0.001					741:749	P < 0.001	741:749	P < 0.001	741:749	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
35932203	4	18	theme	0.366 kg	727:734	arg1	d-1					736:738	0.366 kg d-1	727:738	0.366 kg d-1 (P < 0.001)	727:750	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
35932203	7	19	dep	contents	1200:1207	arg1	fat					1210:1212	fat	1210:1212	fat	1210:1212	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	7	19	dep	contents	1200:1207	arg1	contents					1200:1207	contents	1200:1207	contents (fat and lactose)	1200:1225	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	7	19	dep	contents	1200:1207	arg1	lactose					1218:1224	lactose	1218:1224	lactose	1218:1224	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	0	20	contain	containing	105:114	arg1	diets					99:103	diets	99:103	diets containing oilseed cakes	99:128	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	0	20	contain	containing	105:114	arg2	cakes					124:128	oilseed cakes	116:128	oilseed cakes	116:128	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	2	21	theme	digestibility	388:400	arg1	coefficient					402:412	apparent digestibility coefficient	379:412	apparent digestibility coefficient (ADC)	379:418	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	21	theme	digestibility	388:400	arg1	ADC					415:417	ADC	415:417	ADC	415:417	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	7	22	dep	components	1156:1165	arg1	components					1156:1165	components	1156:1165	components (fat, protein, and lactose)	1156:1193	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	7	22	dep	components	1156:1165	arg1	protein					1173:1179	protein	1173:1179	protein	1173:1179	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	7	22	dep	components	1156:1165	arg1	lactose					1186:1192	lactose	1186:1192	lactose	1186:1192	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	7	22	dep	components	1156:1165	arg1	fat					1168:1170	fat	1168:1170	fat	1168:1170	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	2	23	theme	dairy	488:492	arg1	cows					494:497	lactating dairy cows	478:497	lactating dairy cows	478:497	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	6	24	theme	extract	984:990	arg1	contents					924:931	The contents	920:931	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets	920:1021	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	2	25	theme	apparent	379:386	arg1	coefficient					402:412	apparent digestibility coefficient	379:412	apparent digestibility coefficient (ADC)	379:418	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	25	theme	apparent	379:386	arg1	ADC					415:417	ADC	415:417	ADC	415:417	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	3	26	theme	cakes	640:644	arg1	types					631:635	18 types	628:635	18 types of cakes evaluated in 1350 cows	628:667	The data set used in the meta-analysis came from 51 studies published between 2009 and 2019, which resulted in 119 studies with 18 types of cakes evaluated in 1350 cows.
35932203	2	27	theme	lactating	478:486	arg1	cows					494:497	lactating dairy cows	478:497	lactating dairy cows	478:497	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	6	28	theme	fiber	954:958	arg1	contents					924:931	The contents	920:931	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets	920:1021	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	7	29	theme	milk	1274:1277	arg1	content					1263:1269	the milk protein content	1246:1269	the milk protein content of milk	1246:1277	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	2	30	theme	dry	423:425	arg1	DM					435:436	DM	435:436	DM	435:436	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	30	theme	dry	423:425	arg1	matter					427:432	dry matter	423:432	dry matter (DM)	423:437	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	7	31	theme	protein	1255:1261	arg1	content					1263:1269	the milk protein content	1246:1269	the milk protein content of milk	1246:1277	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	0	32	theme	oilseed	116:122	arg1	cakes					124:128	oilseed cakes	116:128	oilseed cakes	116:128	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	3	33	dep	set	509:511	arg1	used					513:516	used	513:516	set used in the meta-analysis came from 51 studies published between 2009 and 2019, which resulted in 119 studies with 18 types of cakes evaluated in 1350 cows	509:667	The data set used in the meta-analysis came from 51 studies published between 2009 and 2019, which resulted in 119 studies with 18 types of cakes evaluated in 1350 cows.
35932203	4	34	theme	matter	704:709	arg1	intake					711:716	dry matter intake	700:716	dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001)	700:750	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
35932203	4	34	theme	matter	704:709	arg1	DMI					719:721	DMI	719:721	DMI	719:721	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
35932203	2	35	from	effects	344:350	arg1	coefficient					402:412	apparent digestibility coefficient	379:412	apparent digestibility coefficient (ADC)	379:418	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	35	from	effects	344:350	arg1	production					448:457	milk production	443:457	milk production	443:457	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	35	from	effects	344:350	arg1	intake					368:373	intake	368:373	intake	368:373	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	35	from	effects	344:350	arg1	ADC					415:417	ADC	415:417	ADC	415:417	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	7	36	with	Cakes	1045:1049	arg1	contents					1056:1063	contents	1056:1063	contents	1056:1063	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	2	37	theme	principal	299:307	arg1	analysis					319:326	principal component analysis	299:326	principal component analysis	299:326	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	0	38	theme	matter	41:46	arg1	Intake					0:5	Intake	0:5	Intake	0:5	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	0	38	theme	matter	41:46	arg1	digestibility					20:32	apparent digestibility	11:32	apparent digestibility	11:32	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	2	39	contain	carry	269:273	arg2	analysis					319:326	principal component analysis	299:326	principal component analysis	299:326	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	39	contain	carry	269:273	arg1	objective					252:260	The objective	248:260	The objective	248:260	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	2	39	contain	carry	269:273	arg2	meta-analysis					281:293	a meta-analysis	279:293	a meta-analysis	279:293	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	5	40	theme	milk	866:869	arg1	content					879:885	The milk protein content	862:885	The milk protein content	862:885	The milk protein content decreased by 0.050% (P < 0.010).
35932203	2	41	dep	intake	368:373	arg1	the					364:366	the	364:366	the	364:366	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	6	42	theme	neutral	936:942	arg1	NDF					961:963	NDF	961:963	NDF	961:963	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	42	theme	neutral	936:942	arg1	fiber					954:958	neutral detergent fiber	936:958	neutral detergent fiber (NDF) (26%-%)	936:972	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	43	theme	diets	1017:1021	arg1	NDF					961:963	NDF	961:963	NDF	961:963	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	43	theme	diets	1017:1021	arg1	fiber					954:958	neutral detergent fiber	936:958	neutral detergent fiber (NDF) (26%-%)	936:972	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	43	theme	diets	1017:1021	arg1	EE					993:994	EE	993:994	EE	993:994	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	43	theme	diets	1017:1021	arg1	%					1002:1002	3%-7%	998:1002	3%-7%	998:1002	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	43	theme	diets	1017:1021	arg1	extract					984:990	ether extract	978:990	ether extract (EE) (3%-7%)	978:1003	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	5	44	theme	protein	871:877	arg1	content					879:885	The milk protein content	862:885	The milk protein content	862:885	The milk protein content decreased by 0.050% (P < 0.010).
35932203	6	45	theme	ether	978:982	arg1	EE					993:994	EE	993:994	EE	993:994	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	45	theme	ether	978:982	arg1	%					1002:1002	3%-7%	998:1002	3%-7%	998:1002	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	45	theme	ether	978:982	arg1	extract					984:990	ether extract	978:990	ether extract (EE) (3%-7%)	978:1003	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	46	theme	cake	1012:1015	arg1	diets					1017:1021	the cake diets	1008:1021	the cake diets	1008:1021	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	7	47	from	corn	1097:1100	arg1	diet					1116:1119	the diet	1112:1119	the diet	1112:1119	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	2	48	from	composition	463:473	arg1	cows					494:497	lactating dairy cows	478:497	lactating dairy cows	478:497	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	0	49	theme	production	54:63	arg1	Intake					0:5	Intake	0:5	Intake	0:5	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	0	49	theme	production	54:63	arg1	digestibility					20:32	apparent digestibility	11:32	apparent digestibility	11:32	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	2	50	from	production	448:457	arg1	cows					494:497	lactating dairy cows	478:497	lactating dairy cows	478:497	The objective was to carry out a meta-analysis and principal component analysis to evaluate the effects of cakes on the intake and apparent digestibility coefficient (ADC) of dry matter (DM) and milk production and composition in lactating dairy cows.
35932203	4	51	theme	dry	700:702	arg1	intake					711:716	dry matter intake	700:716	dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001)	700:750	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
35932203	4	51	theme	dry	700:702	arg1	DMI					719:721	DMI	719:721	DMI	719:721	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
35932203	6	52	dep	fiber	954:958	arg1	%					971:971	%	971:971	%	971:971	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	6	52	dep	fiber	954:958	arg1	%					969:969	26%	967:969	26%	967:969	The contents of neutral detergent fiber (NDF) (26%-%) and ether extract (EE) (3%-7%) of the cake diets did not affect ADCDM.
35932203	0	53	theme	milk	49:52	arg1	production					54:63	milk production	49:63	milk production	49:63	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	3	54	theme	119	611:613	arg1	studies					615:621	119 studies	611:621	119 studies with 18 types of cakes evaluated in 1350 cows	611:667	The data set used in the meta-analysis came from 51 studies published between 2009 and 2019, which resulted in 119 studies with 18 types of cakes evaluated in 1350 cows.
35932203	0	55	theme	apparent	11:18	arg1	digestibility					20:32	apparent digestibility	11:32	apparent digestibility	11:32	Intake and apparent digestibility of dry matter, milk production, and composition of cows fed with diets containing oilseed cakes: A meta-analysis.
35932203	7	56	from	soy	1105:1107	arg1	diet					1116:1119	the diet	1112:1119	the diet	1112:1119	Cakes with contents between 10% and 30% can replace corn or soy in the diet without affecting milk production, components (fat, protein, and lactose), and contents (fat and lactose), but it can reduce the milk protein content of milk.
35932203	4	57	theme	body	790:793	arg1	weight					795:800	the animal's body weight	777:800	the animal's body weight	777:800	Cows fed with cakes increased dry matter intake (DMI) by 0.366 kg d-1 (P < 0.001) and DMI as a function of the animal's body weight by 0.103% (P < 0.0001) compared with the diet without cake.
36594593	3	0	theme	mg	975:976	arg1	acid					956:959	ferulic acid	948:959	ferulic acid (12.00 ± 0.20 mg g-1)	948:981	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	0	theme	mg	975:976	arg1	g-1					978:980	12.00 ± 0.20 mg g-1	962:980	12.00 ± 0.20 mg g-1	962:980	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	1	1	theme	important	246:254	arg1	plant					288:292	an important medicinal and edible homologous plant	243:292	an important medicinal and edible homologous plant	243:292	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	1	1	theme	important	246:254	arg1	Thyme					213:217	Thyme	213:217	Thyme (Thymus vulgaris L.)	213:238	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	7	2	theme	mg	1926:1927	arg1	dose					1914:1917	the dose	1910:1917	the dose of 100 mg per kg bw	1910:1937	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	3	3	theme	mg	900:901	arg1	apigenin-7-O-glucuronide					861:884	apigenin-7-O-glucuronide	861:884	apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1)	861:906	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	3	theme	mg	900:901	arg1	g-1					903:905	21.06 ± 0.68 mg g-1	887:905	21.06 ± 0.68 mg g-1	887:905	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	7	4	theme	experimental	2015:2026	arg1	groups					2028:2033	the other two TP experimental groups	1998:2033	the other two TP experimental groups	1998:2033	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	0	5	theme	inflammasome	190:201	arg1	pathways					203:210	TLR4/NF-κB-NLRP3 inflammasome pathways	173:210	TLR4/NF-κB-NLRP3 inflammasome pathways	173:210	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	3	6	theme	±	893:893	arg1	apigenin-7-O-glucuronide					861:884	apigenin-7-O-glucuronide	861:884	apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1)	861:906	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	6	theme	±	893:893	arg1	g-1					903:905	21.06 ± 0.68 mg g-1	887:905	21.06 ± 0.68 mg g-1	887:905	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	5	7	theme	epithelial	1498:1507	arg1	integrity					1514:1522	intestinal epithelial cell integrity	1487:1522	intestinal epithelial cell integrity	1487:1522	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	7	8	theme	mitigated	1951:1959	arg1	effects					1961:1967	weak mitigated effects	1946:1967	weak mitigated effects on the above symptoms	1946:1989	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	3	9	theme	gallic	909:914	arg1	acid					916:919	gallic acid	909:919	gallic acid (13.80 ± 0.73 mg g-1)	909:941	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	9	theme	gallic	909:914	arg1	g-1					938:940	13.80 ± 0.73 mg g-1	922:940	13.80 ± 0.73 mg g-1	922:940	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	5	10	theme	tight	1440:1444	arg1	expression					1463:1472	tight junction protein expression	1440:1472	tight junction protein expression	1440:1472	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	0	11	theme	barrier	114:120	arg1	damage					122:127	intestinal barrier damage	103:127	intestinal barrier damage	103:127	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	5	12	with	treatment	1356:1364	arg1	TP					1371:1372	TP	1371:1372	TP	1371:1372	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	6	13	theme	acids	1680:1684	arg1	synthesis					1649:1657	the synthesis	1645:1657	the synthesis of short chain fatty acids (SCFAs)	1645:1692	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	4	14	theme	alleviating	1138:1148	arg1	inflammation					1161:1172	alleviating intestinal inflammation	1138:1172	alleviating intestinal inflammation	1138:1172	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	1	15	theme	Thymus	220:225	arg1	Thyme					213:217	Thyme	213:217	Thyme (Thymus vulgaris L.)	213:238	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	1	15	theme	Thymus	220:225	arg1	L.					236:237	Thymus vulgaris L.	220:237	Thymus vulgaris L.	220:237	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	0	16	theme	gut	141:143	arg1	microbiota					145:154	gut microbiota	141:154	gut microbiota	141:154	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	2	17	theme	polyphenols	561:571	arg1	mechanism					542:550	mechanism	542:550	mechanism	542:550	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	2	17	theme	polyphenols	561:571	arg1	effect					531:536	the intervention effect	514:536	the intervention effect	514:536	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	8	18	theme	epithelial	2283:2292	arg1	barrier					2294:2300	the intestinal epithelial barrier	2268:2300	the intestinal epithelial barrier	2268:2300	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	4	19	theme	NLRP3	1224:1228	arg1	consistency					1195:1205	the consistency	1191:1205	the consistency of the decreased NLRP3 and TLR4 proteins	1191:1246	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	7	20	theme	certain	2134:2140	arg1	dose					2142:2145	a certain dose	2132:2145	a certain dose of TP	2132:2151	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	4	21	theme	TLR4	1234:1237	arg1	proteins					1239:1246	TLR4 proteins	1234:1246	TLR4 proteins	1234:1246	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	0	22	theme	DSS-induced	50:60	arg1	colitis					73:79	DSS-induced ulcerative colitis	50:79	DSS-induced ulcerative colitis of mice	50:87	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	3	23	theme	80.33	803:807	arg1	±					809:809	±	809:809	±	809:809	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	8	24	theme	inflammasome	2237:2248	arg1	pathways					2250:2257	the TLR4/NLRP3-NF-κB inflammasome pathways	2216:2257	the TLR4/NLRP3-NF-κB inflammasome pathways	2216:2257	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	4	25	from	effects	1127:1133	arg1	inflammation					1161:1172	alleviating intestinal inflammation	1138:1172	alleviating intestinal inflammation	1138:1172	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	2	26	theme	thyme	555:559	arg1	polyphenols					561:571	thyme polyphenols	555:571	thyme polyphenols (TP)	555:576	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	2	26	theme	thyme	555:559	arg1	TP					574:575	TP	574:575	TP	574:575	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	6	27	theme	harmful	1717:1723	arg1	bacteria					1725:1732	harmful bacteria	1717:1732	harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process	1717:1850	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	6	27	theme	harmful	1717:1723	arg1	Shigella					1756:1763	Escherichia Shigella	1744:1763	Escherichia Shigella	1744:1763	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	6	27	theme	harmful	1717:1723	arg1	stricto					1801:1807	Clostridium sensu stricto 1	1783:1809	Clostridium sensu stricto 1 associated with the inflammatory process	1783:1850	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	6	27	theme	harmful	1717:1723	arg1	Muribaculum					1766:1776	Muribaculum	1766:1776	Muribaculum	1766:1776	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	3	28	theme	2.09	772:775	arg1	mg					777:778	mg	777:778	mg	777:778	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	2	29	with	combination	622:632	arg1	mice					681:684	dextran sulfate sodium salt (DSS)-induced mice	639:684	dextran sulfate sodium salt (DSS)-induced mice colitis model	639:698	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	3	30	theme	±	928:928	arg1	acid					916:919	gallic acid	909:919	gallic acid (13.80 ± 0.73 mg g-1)	909:941	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	30	theme	±	928:928	arg1	g-1					938:940	13.80 ± 0.73 mg g-1	922:940	13.80 ± 0.73 mg g-1	922:940	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	2	31	theme	intervention	518:529	arg1	effect					531:536	the intervention effect	514:536	the intervention effect	514:536	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	7	32	theme	beneficial	2104:2113	arg1	effects					2115:2121	such beneficial effects	2099:2121	such beneficial effects	2099:2121	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	7	33	theme	promising	2050:2058	arg1	potential					2072:2080	similar promising therapeutic potential	2042:2080	similar promising therapeutic potential	2042:2080	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	8	34	theme	disordered	2319:2328	arg1	microbiota					2334:2343	the disordered gut microbiota	2315:2343	the disordered gut microbiota	2315:2343	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	0	35	theme	mice	84:87	arg1	colitis					73:79	DSS-induced ulcerative colitis	50:79	DSS-induced ulcerative colitis of mice	50:87	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	1	36	theme	extracts	349:356	arg1	bioactivity					319:329	bioactivity	319:329	bioactivity	319:329	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	1	36	theme	extracts	349:356	arg1	composition					303:313	composition	303:313	composition	303:313	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	4	37	theme	experimental	1052:1063	arg1	mice					1065:1068	these experimental mice	1046:1068	these experimental mice	1046:1068	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	3	38	theme	1.74	811:814	arg1	±					809:809	±	809:809	±	809:809	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	6	39	theme	sensu	1795:1799	arg1	stricto					1801:1807	Clostridium sensu stricto 1	1783:1809	Clostridium sensu stricto 1 associated with the inflammatory process	1783:1850	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	4	40	theme	NF-κB	1301:1305	arg1	pathway					1330:1336	NF-κB inflammatory signaling pathway	1301:1336	NF-κB inflammatory signaling pathway	1301:1336	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	0	41	theme	Thyme	0:4	arg1	polyphenols					27:37	Thyme (Thymus vulgaris L.) polyphenols	0:37	Thyme (Thymus vulgaris L.) polyphenols	0:37	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	1	42	theme	edible	270:275	arg1	plant					288:292	an important medicinal and edible homologous plant	243:292	an important medicinal and edible homologous plant	243:292	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	1	42	theme	edible	270:275	arg1	Thyme					213:217	Thyme	213:217	Thyme (Thymus vulgaris L.)	213:238	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	3	43	theme	mg	935:936	arg1	acid					916:919	gallic acid	909:919	gallic acid (13.80 ± 0.73 mg g-1)	909:941	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	43	theme	mg	935:936	arg1	g-1					938:940	13.80 ± 0.73 mg g-1	922:940	13.80 ± 0.73 mg g-1	922:940	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	5	44	theme	junction	1446:1453	arg1	expression					1463:1472	tight junction protein expression	1440:1472	tight junction protein expression	1440:1472	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	0	45	theme	Thymus	7:12	arg1	Thyme					0:4	Thyme	0:4	Thyme (Thymus vulgaris L.) polyphenols	0:37	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	0	45	theme	Thymus	7:12	arg1	L.					23:24	Thymus vulgaris L.	7:24	Thymus vulgaris L.	7:24	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	4	46	theme	pro-inflammatory	1262:1277	arg1	cytokine					1279:1286	pro-inflammatory cytokine	1262:1286	pro-inflammatory cytokine secretion	1262:1296	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	3	47	theme	TP	742:743	arg1	scutellarin					750:760	scutellarin	750:760	scutellarin (160.68 ± 2.09 mg g-1)	750:783	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	47	theme	TP	742:743	arg1	substances					728:737	the main substances	719:737	the main substances of TP	719:743	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	48	theme	mg	852:853	arg1	scutellarein					825:836	scutellarein	825:836	scutellarein (56.53 ± 1.32 mg g-1)	825:858	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	48	theme	mg	852:853	arg1	g-1					855:857	56.53 ± 1.32 mg g-1	839:857	56.53 ± 1.32 mg g-1	839:857	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	49	theme	0.20	970:973	arg1	±					968:968	±	968:968	±	968:968	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	50	theme	±	845:845	arg1	scutellarein					825:836	scutellarein	825:836	scutellarein (56.53 ± 1.32 mg g-1)	825:858	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	50	theme	±	845:845	arg1	g-1					855:857	56.53 ± 1.32 mg g-1	839:857	56.53 ± 1.32 mg g-1	839:857	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	7	51	theme	above	1976:1980	arg1	symptoms					1982:1989	the above symptoms	1972:1989	the above symptoms	1972:1989	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	8	52	theme	dietary	2391:2397	arg1	TP					2367:2368	TP	2367:2368	TP	2367:2368	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	8	52	theme	dietary	2391:2397	arg1	strategy					2399:2406	a promising dietary strategy	2379:2406	a promising dietary strategy for UC	2379:2413	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	1	53	theme	vulgaris	227:234	arg1	Thyme					213:217	Thyme	213:217	Thyme (Thymus vulgaris L.)	213:238	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	1	53	theme	vulgaris	227:234	arg1	L.					236:237	Thymus vulgaris L.	220:237	Thymus vulgaris L.	220:237	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	3	54	theme	12.00	962:966	arg1	±					968:968	±	968:968	±	968:968	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	6	55	theme	short	1662:1666	arg1	SCFAs					1687:1691	SCFAs	1687:1691	SCFAs	1687:1691	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	6	55	theme	short	1662:1666	arg1	acids					1680:1684	short chain fatty acids	1662:1684	short chain fatty acids (SCFAs)	1662:1692	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	6	56	theme	inflammatory	1831:1842	arg1	process					1844:1850	the inflammatory process	1827:1850	the inflammatory process	1827:1850	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	4	57	theme	signaling	1320:1328	arg1	pathway					1330:1336	NF-κB inflammatory signaling pathway	1301:1336	NF-κB inflammatory signaling pathway	1301:1336	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	2	58	from	mechanism	542:550	arg1	UC					601:602	UC	601:602	UC	601:602	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	2	58	from	mechanism	542:550	arg1	colitis					592:598	ulcerative colitis	581:598	ulcerative colitis (UC)	581:603	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	0	59	theme	TLR4/NF-κB-NLRP3	173:188	arg1	pathways					203:210	TLR4/NF-κB-NLRP3 inflammasome pathways	173:210	TLR4/NF-κB-NLRP3 inflammasome pathways	173:210	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	2	60	theme	ulcerative	581:590	arg1	UC					601:602	UC	601:602	UC	601:602	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	2	60	theme	ulcerative	581:590	arg1	colitis					592:598	ulcerative colitis	581:598	ulcerative colitis (UC)	581:603	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	4	61	theme	similar	1119:1125	arg1	effects					1127:1133	similar effects	1119:1133	similar effects on alleviating intestinal inflammation	1119:1172	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	1	62	theme	medicinal	256:264	arg1	plant					288:292	an important medicinal and edible homologous plant	243:292	an important medicinal and edible homologous plant	243:292	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	1	62	theme	medicinal	256:264	arg1	Thyme					213:217	Thyme	213:217	Thyme (Thymus vulgaris L.)	213:238	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	7	63	theme	weak	1946:1949	arg1	effects					1961:1967	weak mitigated effects	1946:1967	weak mitigated effects on the above symptoms	1946:1989	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	6	64	dep	elevated	1699:1706	arg1	whereas					1709:1715	whereas	1709:1715	whereas	1709:1715	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	5	65	theme	mg	1398:1399	arg1	doses					1377:1381	doses	1377:1381	doses of 200 and 400 mg per kg bw	1377:1409	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	5	66	theme	protein	1455:1461	arg1	expression					1463:1472	tight junction protein expression	1440:1472	tight junction protein expression	1440:1472	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	7	67	theme	TP	2150:2151	arg1	dose					2142:2145	a certain dose	2132:2145	a certain dose of TP	2132:2151	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	2	68	theme	-induced	672:679	arg1	mice					681:684	dextran sulfate sodium salt (DSS)-induced mice	639:684	dextran sulfate sodium salt (DSS)-induced mice colitis model	639:698	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	6	69	theme	fatty	1674:1678	arg1	SCFAs					1687:1691	SCFAs	1687:1691	SCFAs	1687:1691	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	6	69	theme	fatty	1674:1678	arg1	acids					1680:1684	short chain fatty acids	1662:1684	short chain fatty acids (SCFAs)	1662:1692	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	4	70	theme	intestinal	1150:1159	arg1	inflammation					1161:1172	alleviating intestinal inflammation	1138:1172	alleviating intestinal inflammation	1138:1172	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	3	71	theme	rosmarinic	786:795	arg1	g-1					819:821	80.33 ± 1.74 mg g-1	803:821	80.33 ± 1.74 mg g-1	803:821	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	71	theme	rosmarinic	786:795	arg1	acid					797:800	rosmarinic acid	786:800	rosmarinic acid (80.33 ± 1.74 mg g-1)	786:822	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	8	72	theme	TLR4/NLRP3-NF-κB	2220:2235	arg1	pathways					2250:2257	the TLR4/NLRP3-NF-κB inflammasome pathways	2216:2257	the TLR4/NLRP3-NF-κB inflammasome pathways	2216:2257	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	3	73	theme	main	723:726	arg1	scutellarin					750:760	scutellarin	750:760	scutellarin (160.68 ± 2.09 mg g-1)	750:783	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	73	theme	main	723:726	arg1	substances					728:737	the main substances	719:737	the main substances of TP	719:743	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	6	74	theme	Escherichia	1744:1754	arg1	Shigella					1756:1763	Escherichia Shigella	1744:1763	Escherichia Shigella	1744:1763	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	4	75	theme	decreased	1214:1222	arg1	NLRP3					1224:1228	the decreased NLRP3	1210:1228	the decreased NLRP3	1210:1228	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	0	76	theme	ulcerative	62:71	arg1	colitis					73:79	DSS-induced ulcerative colitis	50:79	DSS-induced ulcerative colitis of mice	50:87	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	6	77	theme	probiotics	1555:1564	arg1	abundany					1543:1550	the abundany	1539:1550	the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs)	1539:1692	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	2	78	theme	colitis	686:692	arg1	model					694:698	colitis model	686:698	dextran sulfate sodium salt (DSS)-induced mice colitis model	639:698	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	7	79	from	effects	1961:1967	arg1	symptoms					1982:1989	the above symptoms	1972:1989	the above symptoms	1972:1989	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	7	80	theme	such	2099:2102	arg1	effects					2115:2121	such beneficial effects	2099:2121	such beneficial effects	2099:2121	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	8	81	theme	gut	2330:2332	arg1	microbiota					2334:2343	the disordered gut microbiota	2315:2343	the disordered gut microbiota	2315:2343	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	3	82	theme	mg	777:778	arg1	scutellarin					750:760	scutellarin	750:760	scutellarin (160.68 ± 2.09 mg g-1)	750:783	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	82	theme	mg	777:778	arg1	g-1					780:782	160.68 ± 2.09 mg g-1	763:782	160.68 ± 2.09 mg g-1	763:782	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	1	83	theme	polyphenol	338:347	arg1	extracts					349:356	its polyphenol extracts	334:356	its polyphenol extracts	334:356	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	8	84	theme	intestinal	2272:2281	arg1	barrier					2294:2300	the intestinal epithelial barrier	2268:2300	the intestinal epithelial barrier	2268:2300	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	1	85	dep	composition	303:313	arg1	the					299:301	the	299:301	the	299:301	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	7	86	theme	therapeutic	2060:2070	arg1	potential					2072:2080	similar promising therapeutic potential	2042:2080	similar promising therapeutic potential	2042:2080	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	3	87	theme	±	770:770	arg1	scutellarin					750:760	scutellarin	750:760	scutellarin (160.68 ± 2.09 mg g-1)	750:783	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	87	theme	±	770:770	arg1	g-1					780:782	160.68 ± 2.09 mg g-1	763:782	160.68 ± 2.09 mg g-1	763:782	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	0	88	theme	intestinal	103:112	arg1	damage					122:127	intestinal barrier damage	103:127	intestinal barrier damage	103:127	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	2	89	dep	mice	681:684	arg1	model					694:698	colitis model	686:698	dextran sulfate sodium salt (DSS)-induced mice colitis model	639:698	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	2	90	from	effect	531:536	arg1	UC					601:602	UC	601:602	UC	601:602	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	2	90	from	effect	531:536	arg1	colitis					592:598	ulcerative colitis	581:598	ulcerative colitis (UC)	581:603	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	4	91	theme	cytokine	1279:1286	arg1	secretion					1288:1296	pro-inflammatory cytokine secretion	1262:1296	pro-inflammatory cytokine secretion	1262:1296	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	1	92	theme	widespread	373:382	arg1	attention					384:392	widespread attention	373:392	widespread attention	373:392	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	3	93	theme	0.73	930:933	arg1	mg					935:936	mg	935:936	mg	935:936	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	94	theme	mg	816:817	arg1	g-1					819:821	80.33 ± 1.74 mg g-1	803:821	80.33 ± 1.74 mg g-1	803:821	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	94	theme	mg	816:817	arg1	acid					797:800	rosmarinic acid	786:800	rosmarinic acid (80.33 ± 1.74 mg g-1)	786:822	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	6	95	theme	Clostridium	1783:1793	arg1	stricto					1801:1807	Clostridium sensu stricto 1	1783:1809	Clostridium sensu stricto 1 associated with the inflammatory process	1783:1850	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	5	96	theme	cell	1509:1512	arg1	integrity					1514:1522	intestinal epithelial cell integrity	1487:1522	intestinal epithelial cell integrity	1487:1522	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	0	97	theme	vulgaris	14:21	arg1	Thyme					0:4	Thyme	0:4	Thyme (Thymus vulgaris L.) polyphenols	0:37	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	0	97	theme	vulgaris	14:21	arg1	L.					23:24	Thymus vulgaris L.	7:24	Thymus vulgaris L.	7:24	Thyme (Thymus vulgaris L.) polyphenols ameliorate DSS-induced ulcerative colitis of mice by mitigating intestinal barrier damage, regulating gut microbiota, and suppressing TLR4/NF-κB-NLRP3 inflammasome pathways.
36594593	1	98	theme	homologous	277:286	arg1	plant					288:292	an important medicinal and edible homologous plant	243:292	an important medicinal and edible homologous plant	243:292	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	1	98	theme	homologous	277:286	arg1	Thyme					213:217	Thyme	213:217	Thyme (Thymus vulgaris L.)	213:238	Thyme (Thymus vulgaris L.) is an important medicinal and edible homologous plant, and the composition and bioactivity of its polyphenol extracts have attracted widespread attention from researchers.
36594593	3	99	theme	ferulic	948:954	arg1	acid					956:959	ferulic acid	948:959	ferulic acid (12.00 ± 0.20 mg g-1)	948:981	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	3	99	theme	ferulic	948:954	arg1	g-1					978:980	12.00 ± 0.20 mg g-1	962:980	12.00 ± 0.20 mg g-1	962:980	It was found that the main substances of TP were scutellarin (160.68 ± 2.09 mg g-1), rosmarinic acid (80.33 ± 1.74 mg g-1), scutellarein (56.53 ± 1.32 mg g-1), apigenin-7-O-glucuronide (21.06 ± 0.68 mg g-1), gallic acid (13.80 ± 0.73 mg g-1), and ferulic acid (12.00 ± 0.20 mg g-1).
36594593	2	100	from	polyphenols	431:441	arg1	thyme					446:450	thyme	446:450	thyme	446:450	In this study, the polyphenols in thyme were separated and identified by UPLC/MS-MS and UPLC-DAD, and the intervention effect and mechanism of thyme polyphenols (TP) on ulcerative colitis (UC) were analyzed in combination with dextran sulfate sodium salt (DSS)-induced mice colitis model.
36594593	7	101	theme	TP	2012:2013	arg1	groups					2028:2033	the other two TP experimental groups	1998:2033	the other two TP experimental groups	1998:2033	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	4	102	theme	proteins	1239:1246	arg1	consistency					1195:1205	the consistency	1191:1205	the consistency of the decreased NLRP3 and TLR4 proteins	1191:1246	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
36594593	5	103	from	doses	1377:1381	arg1	treatment					1356:1364	the treatment	1352:1364	the treatment with TP at doses of 200 and 400 mg per kg bw	1352:1409	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	7	104	theme	other	2002:2006	arg1	groups					2028:2033	the other two TP experimental groups	1998:2033	the other two TP experimental groups	1998:2033	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	8	105	theme	promising	2381:2389	arg1	TP					2367:2368	TP	2367:2368	TP	2367:2368	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	8	105	theme	promising	2381:2389	arg1	strategy					2399:2406	a promising dietary strategy	2379:2406	a promising dietary strategy for UC	2379:2413	These results indicated that TP could suppress the TLR4/NLRP3-NF-κB inflammasome pathways, protect the intestinal epithelial barrier, and remodel the disordered gut microbiota, which suggested that TP might be a promising dietary strategy for UC.
36594593	6	106	theme	chain	1668:1672	arg1	SCFAs					1687:1691	SCFAs	1687:1691	SCFAs	1687:1691	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	6	106	theme	chain	1668:1672	arg1	acids					1680:1684	short chain fatty acids	1662:1684	short chain fatty acids (SCFAs)	1662:1692	Consistently, the abundany of probiotics including Blautia, Bacteroides, Romboutsia, and Faecalibaculum associated with the synthesis of short chain fatty acids (SCFAs) were elevated, whereas harmful bacteria including Escherichia Shigella, Muribaculum, and Clostridium sensu stricto 1 associated with the inflammatory process were significantly inhibited.
36594593	7	107	theme	similar	2042:2048	arg1	potential					2072:2080	similar promising therapeutic potential	2042:2080	similar promising therapeutic potential	2042:2080	Notably, TP supplemented at the dose of 100 mg per kg bw showed weak mitigated effects on the above symptoms, while the other two TP experimental groups showed similar promising therapeutic potential, suggesting that such beneficial effects required a certain dose of TP to be achieved.
36594593	5	108	theme	intestinal	1487:1496	arg1	integrity					1514:1522	intestinal epithelial cell integrity	1487:1522	intestinal epithelial cell integrity	1487:1522	Furthermore, the treatment with TP at doses of 200 and 400 mg per kg bw both effectively upregulated tight junction protein expression and enhanced intestinal epithelial cell integrity.
36594593	4	109	theme	inflammatory	1307:1318	arg1	pathway					1330:1336	NF-κB inflammatory signaling pathway	1301:1336	NF-κB inflammatory signaling pathway	1301:1336	TP and sulfasalazine, which were respectively supplemented to these experimental mice at 200 mg per kg bw and 100 mg per kg bw, showed similar effects on alleviating intestinal inflammation, as indicated by the consistency of the decreased NLRP3 and TLR4 proteins and inhibited pro-inflammatory cytokine secretion in NF-κB inflammatory signaling pathway.
35267932	2	0	from	mechanisms	381:390	arg1	organism					415:422	the human organism	405:422	the human organism	405:422	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	5	1	theme	appetite	1044:1051	arg1	regulation					1053:1062	appetite regulation	1044:1062	appetite regulation	1044:1062	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	4	2	theme	murine	635:640	arg1	model					642:646	the murine model	631:646	the murine model	631:646	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	0	3	theme	Food	68:71	arg1	Effectors					39:47	Effectors	39:47	Effectors	39:47	Chicory: Understanding the Effects and Effectors of This Functional Food.
35267932	0	3	theme	Food	68:71	arg1	Effects					27:33	Effects	27:33	Effects	27:33	Chicory: Understanding the Effects and Effectors of This Functional Food.
35267932	3	4	from	study	452:456	arg1	interested					467:476	interested	467:476	interested	467:476	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	5	5	from	role	1036:1039	arg1	regulation					1053:1062	appetite regulation	1044:1062	appetite regulation	1044:1062	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	3	6	from	acids	568:572	arg1	interested					467:476	interested	467:476	interested	467:476	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	2	7	from	action	395:400	arg1	organism					415:422	the human organism	405:422	the human organism	405:422	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	4	8	theme	in	760:761	arg1	observations					769:780	in vitro observations	760:780	in vitro observations for different responses	760:804	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	4	9	theme	hormone	699:705	arg1	assay					707:711	a metabolic hormone assay	687:711	a metabolic hormone assay	687:711	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	3	10	attach	present	521:527	arg2	molecules					511:519	molecules	511:519	molecules present in chicory root	511:543	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	10	attach	present	521:527	arg1	root					540:543	chicory root	532:543	chicory root	532:543	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	5	11	theme	important	1026:1034	arg1	role					1036:1039	an important role	1023:1039	a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation	827:1062	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	4	12	theme	metabolic	689:697	arg1	assay					707:711	a metabolic hormone assay	687:711	a metabolic hormone assay	687:711	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	2	13	theme	different	275:283	arg1	classes					285:291	the different classes	271:291	the different classes	271:291	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	6	14	theme	significant	1067:1077	arg1	activity					1089:1096	A significant prebiotic activity	1065:1096	A significant prebiotic activity	1065:1096	A significant prebiotic activity was also identified.
35267932	3	15	from	fructose	546:553	arg1	interested					467:476	interested	467:476	interested	467:476	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	2	16	theme	plant	346:350	arg1	composition					326:336	the composition	322:336	the composition of this plant	322:350	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	5	17	theme	large	829:833	arg1	number					835:840	a large number	827:840	a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation	827:1062	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	4	18	from	experiments	616:626	arg1	model					642:646	the murine model	631:646	the murine model	631:646	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	7	19	theme	tested	1261:1266	arg1	molecules					1268:1276	tested molecules	1261:1276	tested molecules	1261:1276	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	2	20	from	organism	415:422	arg1	mechanisms					381:390	the molecular mechanisms	367:390	the molecular mechanisms of action on the human organism	367:422	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	4	21	theme	different	786:794	arg1	responses					796:804	different responses	786:804	different responses	786:804	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	3	22	from	lactones	593:600	arg1	interested					467:476	interested	467:476	interested	467:476	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	1	23	theme	Industrial	74:83	arg1	chicory					85:91	Industrial chicory	74:91	Industrial chicory	74:91	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	1	23	theme	Industrial	74:83	arg1	subject					106:112	the subject	102:112	the subject of numerous studies, most of which provide clinical observations on its health effects	102:199	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	1	24	theme	clinical	157:164	arg1	observations					166:177	clinical observations	157:177	clinical observations on its health effects	157:199	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	2	25	theme	action	395:400	arg1	mechanisms					381:390	the molecular mechanisms	367:390	the molecular mechanisms of action on the human organism	367:422	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	5	26	theme	hypoglycemic	994:1005	arg1	activity					916:923	a pro-apoptotic activity	900:923	a pro-apoptotic activity	900:923	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	5	26	theme	hypoglycemic	994:1005	arg1	effect					1007:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	3	27	theme	molecules	511:519	arg1	classes					500:506	three molecules or classes	481:506	classes	500:506	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	27	theme	molecules	511:519	arg1	acids					568:572	chlorogenic acids	556:572	chlorogenic acids	556:572	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	27	theme	molecules	511:519	arg1	molecules					487:495	three molecules or classes	481:506	molecules	487:495	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	27	theme	molecules	511:519	arg1	lactones					593:600	sesquiterpene lactones	579:600	sesquiterpene lactones	579:600	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	27	theme	molecules	511:519	arg1	fructose					546:553	fructose	546:553	fructose	546:553	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	28	from	present	521:527	arg1	root					540:543	chicory root	532:543	chicory root	532:543	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	7	29	theme	specific	1291:1298	arg1	role					1300:1303	a specific role	1289:1303	a specific role for these different effects	1289:1331	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	7	30	theme	molecules	1268:1276	arg1	classes					1250:1256	the other classes	1240:1256	the other classes of tested molecules	1240:1276	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	5	31	theme	antioxidant	963:973	arg1	activity					916:923	a pro-apoptotic activity	900:923	a pro-apoptotic activity	900:923	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	5	31	theme	antioxidant	963:973	arg1	effect					1007:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	7	32	theme	different	1315:1323	arg1	effects					1325:1331	these different effects	1309:1331	these different effects	1309:1331	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	5	33	theme	molecules	877:885	arg1	classes					866:872	all these classes	856:872	all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	856:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	2	34	theme	molecular	371:379	arg1	mechanisms					381:390	the molecular mechanisms	367:390	the molecular mechanisms of action on the human organism	367:422	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	4	35	theme	nutrigenomic	664:675	arg1	analysis					677:684	a nutrigenomic analysis	662:684	a nutrigenomic analysis	662:684	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	5	36	theme	classes	866:872	arg1	effects					845:851	effects	845:851	effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	845:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	4	37	dep	in	760:761	arg1	vitro					763:767	vitro	763:767	vitro	763:767	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	7	38	theme	recorded	1216:1223	arg1	responses					1225:1233	recorded responses	1216:1233	recorded responses	1216:1233	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	3	39	theme	sesquiterpene	579:591	arg1	lactones					593:600	sesquiterpene lactones	579:600	sesquiterpene lactones	579:600	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	40	theme	chicory	532:538	arg1	root					540:543	chicory root	532:543	chicory root	532:543	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	41	from	root	540:543	arg1	present					521:527	present	521:527	present	521:527	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	1	42	theme	health	186:191	arg1	effects					193:199	its health effects	182:199	its health effects	182:199	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	7	43	theme	responses	1225:1233	arg1	%					1211:1211	approximately 83%	1195:1211	approximately 83% of recorded responses	1195:1233	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	7	43	theme	responses	1225:1233	arg1	responses					1225:1233	recorded responses	1216:1233	recorded responses	1216:1233	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	5	44	theme	antimicrobial	948:960	arg1	activity					916:923	a pro-apoptotic activity	900:923	a pro-apoptotic activity	900:923	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	5	44	theme	antimicrobial	948:960	arg1	effect					1007:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	2	45	theme	human	409:413	arg1	organism					415:422	the human organism	405:422	the human organism	405:422	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	3	46	theme	present	521:527	arg1	molecules					511:519	molecules	511:519	molecules present in chicory root	511:543	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	2	47	theme	molecules	296:304	arg1	roots					262:266	the roots	258:266	the roots	258:266	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	2	47	theme	molecules	296:304	arg1	classes					285:291	the different classes	271:291	the different classes	271:291	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	0	48	dep	Effects	27:33	arg1	the					23:25	the	23:25	the	23:25	Chicory: Understanding the Effects and Effectors of This Functional Food.
35267932	5	49	theme	effects	845:851	arg1	number					835:840	a large number	827:840	a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation	827:1062	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	5	50	theme	anti-inflammatory	929:945	arg1	activity					916:923	a pro-apoptotic activity	900:923	a pro-apoptotic activity	900:923	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	5	50	theme	anti-inflammatory	929:945	arg1	effect					1007:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	7	51	theme	estimated	1342:1350	arg1	contribution					1352:1363	an estimated contribution	1339:1363	an estimated contribution of 23-24%	1339:1373	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	5	52	theme	hypolipidemic	976:988	arg1	activity					916:923	a pro-apoptotic activity	900:923	a pro-apoptotic activity	900:923	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	5	52	theme	hypolipidemic	976:988	arg1	effect					1007:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	4	53	theme	gut	719:721	arg1	analysis					734:741	a gut microbiota analysis	717:741	a gut microbiota analysis	717:741	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	3	54	from	classes	500:506	arg1	interested					467:476	interested	467:476	interested	467:476	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	5	55	theme	pro-apoptotic	902:914	arg1	activity					916:923	a pro-apoptotic activity	900:923	a pro-apoptotic activity	900:923	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	5	55	theme	pro-apoptotic	902:914	arg1	effect					1007:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect	926:1012	We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation.
35267932	3	56	theme	chlorogenic	556:566	arg1	acids					568:572	chlorogenic acids	556:572	chlorogenic acids	556:572	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	1	57	theme	numerous	117:124	arg1	studies					126:132	numerous studies	117:132	numerous studies	117:132	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	7	58	theme	other	1244:1248	arg1	classes					1250:1256	the other classes	1240:1256	the other classes of tested molecules	1240:1276	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	0	59	theme	Functional	57:66	arg1	Food					68:71	This Functional Food	52:71	This Functional Food	52:71	Chicory: Understanding the Effects and Effectors of This Functional Food.
35267932	3	60	from	molecules	487:495	arg1	interested					467:476	interested	467:476	interested	467:476	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	2	61	theme	roasted	220:226	arg1	it					210:211	it	210:211	it	210:211	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	2	61	theme	roasted	220:226	arg1	root					228:231	the roasted root	216:231	the roasted root	216:231	Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete.
35267932	1	62	from	observations	166:177	arg1	effects					193:199	its health effects	182:199	its health effects	182:199	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	1	63	theme	studies	126:132	arg1	chicory					85:91	Industrial chicory	74:91	Industrial chicory	74:91	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	1	63	theme	studies	126:132	arg1	subject					106:112	the subject	102:112	the subject of numerous studies, most of which provide clinical observations on its health effects	102:199	Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects.
35267932	7	64	theme	%	1373:1373	arg1	contribution					1352:1363	an estimated contribution	1339:1363	an estimated contribution of 23-24%	1339:1373	Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23-24%.
35267932	6	65	theme	prebiotic	1079:1087	arg1	activity					1089:1096	A significant prebiotic activity	1065:1096	A significant prebiotic activity	1065:1096	A significant prebiotic activity was also identified.
35267932	4	66	theme	microbiota	723:732	arg1	analysis					734:741	a gut microbiota analysis	717:741	a gut microbiota analysis	717:741	We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses.
35267932	3	67	from	interested	467:476	arg1	acids					568:572	chlorogenic acids	556:572	chlorogenic acids	556:572	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	67	from	interested	467:476	arg1	classes					500:506	three molecules or classes	481:506	classes	500:506	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	67	from	interested	467:476	arg1	fructose					546:553	fructose	546:553	fructose	546:553	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	67	from	interested	467:476	arg1	lactones					593:600	sesquiterpene lactones	579:600	sesquiterpene lactones	579:600	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	67	from	interested	467:476	arg1	molecules					487:495	three molecules or classes	481:506	molecules	487:495	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
35267932	3	67	from	interested	467:476	arg1	study					452:456	this study	447:456	this study	447:456	In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones.
36444019	0	0	theme	bile	68:71	arg1	acids					73:77	bile acids	68:77	bile acids	68:77	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	3	1	theme	primary	713:719	arg1	acids					726:730	primary bile acids	713:730	primary bile acids (CA, CDCA, β-MCA)	713:748	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	3	2	theme	acids	726:730	arg1	production					699:708	the production	695:708	the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA)	695:785	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	1	3	theme	rats	299:302	arg1	rats					299:302	rats	299:302	rats	299:302	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	3	theme	rats	299:302	arg1	groups					289:294	four groups	284:294	four groups of rats	284:302	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	3	4	theme	TCA	816:818	arg1	contents					804:811	the contents	800:811	the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats	800:881	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	1	5	theme	small	214:218	arg1	flora					231:235	the small intestinal flora	210:235	the small intestinal flora	210:235	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	0	6	from	Effect	0:5	arg1	flora					58:62	small intestinal flora	41:62	small intestinal flora	41:62	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	0	6	from	Effect	0:5	arg1	acids					73:77	bile acids	68:77	bile acids	68:77	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	1	7	theme	blood	399:403	arg1	profiles					411:418	blood lipid profiles	399:418	blood lipid profiles	399:418	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	8	theme	intestinal	220:229	arg1	flora					231:235	the small intestinal flora	210:235	the small intestinal flora	210:235	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	2	9	theme	Romboutsia	542:551	arg1	growth					532:537	the growth	528:537	the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group	528:646	LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats.
36444019	1	10	theme	lipid	405:409	arg1	profiles					411:418	blood lipid profiles	399:418	blood lipid profiles	399:418	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	11	theme	hyperlipidemic	493:506	arg1	rats					508:511	hyperlipidemic rats	493:511	hyperlipidemic rats	493:511	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	12	theme	flora	231:235	arg1	structure					197:205	the structure	193:205	the structure of the small intestinal flora	193:235	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	12	theme	flora	231:235	arg1	acids					246:250	bile acids	241:250	bile acids	241:250	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	3	13	theme	LCA-3-S	842:848	arg1	contents					804:811	the contents	800:811	the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats	800:881	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	2	14	theme	norank_f__Eubacterium_coprostanoligenes_group	602:646	arg1	growth					532:537	the growth	528:537	the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group	528:646	LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats.
36444019	1	15	from	effect	476:481	arg1	rats					508:511	hyperlipidemic rats	493:511	hyperlipidemic rats	493:511	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	16	theme	lotus	154:158	arg1	LRS					183:185	LRS	183:185	LRS	183:185	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	16	theme	lotus	154:158	arg1	starch					175:180	lotus seed resistant starch	154:180	lotus seed resistant starch (LRS)	154:186	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	0	17	theme	hyperlipidemic	82:95	arg1	rats					97:100	hyperlipidemic rats	82:100	hyperlipidemic rats	82:100	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	1	18	theme	relationship	319:330	arg1	diagram					340:346	a relationship network diagram	317:346	a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles	317:418	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	2	19	theme	norank_f__Muribaculaceae	573:596	arg1	growth					532:537	the growth	528:537	the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group	528:646	LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats.
36444019	3	20	theme	isoLCA	834:839	arg1	contents					804:811	the contents	800:811	the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats	800:881	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	1	21	theme	seed	160:163	arg1	LRS					183:185	LRS	183:185	LRS	183:185	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	21	theme	seed	160:163	arg1	starch					175:180	lotus seed resistant starch	154:180	lotus seed resistant starch (LRS)	154:186	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	22	theme	network	332:338	arg1	diagram					340:346	a relationship network diagram	317:346	a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles	317:418	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	5	23	theme	intestinal	1132:1141	arg1	flora					1143:1147	small intestinal flora	1126:1147	small intestinal flora	1126:1147	In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
36444019	3	24	theme	Meanwhile	672:680	arg1	LRS					682:684	Meanwhile LRS	672:684	Meanwhile LRS	672:684	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	1	25	theme	resistant	165:173	arg1	LRS					183:185	LRS	183:185	LRS	183:185	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	25	theme	resistant	165:173	arg1	starch					175:180	lotus seed resistant starch	154:180	lotus seed resistant starch (LRS)	154:186	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	5	26	theme	small	1126:1130	arg1	flora					1143:1147	small intestinal flora	1126:1147	small intestinal flora	1126:1147	In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
36444019	2	27	theme	Blautia	564:570	arg1	growth					532:537	the growth	528:537	the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group	528:646	LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats.
36444019	2	28	theme	hyperlipidemic	651:664	arg1	rats					666:669	hyperlipidemic rats	651:669	hyperlipidemic rats	651:669	LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats.
36444019	3	29	theme	hyperlipidemic	863:876	arg1	rats					878:881	hyperlipidemic rats	863:881	hyperlipidemic rats	863:881	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	0	30	theme	seed	16:19	arg1	starch					31:36	lotus seed resistant starch	10:36	lotus seed resistant starch	10:36	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	1	31	theme	bile	241:244	arg1	acids					246:250	bile acids	241:250	bile acids	241:250	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	32	theme	microbial	433:441	arg1	mechanism					443:451	a microbial mechanism	431:451	a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats	431:511	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	3	33	from	contents	804:811	arg1	rats					878:881	hyperlipidemic rats	863:881	hyperlipidemic rats	863:881	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	0	34	theme	lotus	10:14	arg1	starch					31:36	lotus seed resistant starch	10:36	lotus seed resistant starch	10:36	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	1	35	dep	structure	197:205	arg1	composition					252:262	composition	252:262	composition	252:262	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	3	36	theme	THDCA	854:858	arg1	contents					804:811	the contents	800:811	the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats	800:881	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	0	37	theme	starch	31:36	arg1	Effect					0:5	Effect	0:5	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.	0:101	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	1	38	theme	Ordinary	103:110	arg1	rats					131:134	Ordinary and hyperlipidemic rats	103:134	Ordinary and hyperlipidemic rats	103:134	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	0	39	theme	resistant	21:29	arg1	starch					31:36	lotus seed resistant starch	10:36	lotus seed resistant starch	10:36	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	3	40	theme	secondary	754:762	arg1	acids					769:773	secondary bile acids	754:773	secondary bile acids (LCA, UDCA)	754:785	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	3	40	theme	secondary	754:762	arg1	UDCA					781:784	UDCA	781:784	UDCA	781:784	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	2	41	dep	Romboutsia	542:551	arg1	Bacillus					554:561	Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group	542:646	Bacillus	554:561	LRS inhibited the growth of Romboutsia, Bacillus, Blautia, norank_f__Muribaculaceae and norank_f__Eubacterium_coprostanoligenes_group in hyperlipidemic rats.
36444019	0	42	from	flora	58:62	arg1	rats					97:100	hyperlipidemic rats	82:100	hyperlipidemic rats	82:100	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	5	43	theme	bile	1200:1203	arg1	acids					1205:1209	bile acids	1200:1209	bile acids in the liver	1200:1222	In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
36444019	0	44	theme	small	41:45	arg1	flora					58:62	small intestinal flora	41:62	small intestinal flora	41:62	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	3	45	theme	bile	764:767	arg1	acids					769:773	secondary bile acids	754:773	secondary bile acids (LCA, UDCA)	754:785	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	3	45	theme	bile	764:767	arg1	UDCA					781:784	UDCA	781:784	UDCA	781:784	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	1	46	theme	bacterial	366:374	arg1	genera					376:381	different bacterial genera	356:381	different bacterial genera	356:381	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	3	47	theme	bile	721:724	arg1	acids					726:730	primary bile acids	713:730	primary bile acids (CA, CDCA, β-MCA)	713:748	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	5	48	theme	blood	1093:1097	arg1	levels					1105:1110	blood lipid levels	1093:1110	blood lipid levels	1093:1110	In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
36444019	5	49	from	acids	1205:1209	arg1	liver					1218:1222	the liver	1214:1222	the liver	1214:1222	In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
36444019	5	50	theme	lipid	1099:1103	arg1	levels					1105:1110	blood lipid levels	1093:1110	blood lipid levels	1093:1110	In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
36444019	1	51	theme	different	356:364	arg1	genera					376:381	different bacterial genera	356:381	different bacterial genera	356:381	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	3	52	dep	CA	733:734	arg1	β-MCA					743:747	β-MCA	743:747	β-MCA	743:747	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	3	52	dep	CA	733:734	arg1	CDCA					737:740	CDCA	737:740	CDCA	737:740	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	1	53	theme	lipid-lowering	461:474	arg1	effect					476:481	the lipid-lowering effect	457:481	the lipid-lowering effect of LRS in hyperlipidemic rats	457:511	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	5	54	theme	cholesterol	1183:1193	arg1	breakdown					1170:1178	the breakdown	1166:1178	the breakdown of cholesterol into bile acids in the liver	1166:1222	In summary, LRS improves blood lipid levels by regulating small intestinal flora and accelerating the breakdown of cholesterol into bile acids in the liver.
36444019	1	55	theme	bile	384:387	arg1	acids					389:393	bile acids	384:393	bile acids	384:393	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	1	56	theme	hyperlipidemic	116:129	arg1	rats					131:134	Ordinary and hyperlipidemic rats	103:134	Ordinary and hyperlipidemic rats	103:134	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	0	57	from	acids	73:77	arg1	rats					97:100	hyperlipidemic rats	82:100	hyperlipidemic rats	82:100	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	3	58	theme	acids	769:773	arg1	production					699:708	the production	695:708	the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA)	695:785	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	0	59	theme	intestinal	47:56	arg1	flora					58:62	small intestinal flora	41:62	small intestinal flora	41:62	Effect of lotus seed resistant starch on small intestinal flora and bile acids in hyperlipidemic rats.
36444019	1	60	theme	LRS	486:488	arg1	effect					476:481	the lipid-lowering effect	457:481	the lipid-lowering effect of LRS in hyperlipidemic rats	457:511	Ordinary and hyperlipidemic rats were gavaged with lotus seed resistant starch (LRS), and the structure of the small intestinal flora and bile acids composition were determined for four groups of rats to construct a relationship network diagram between different bacterial genera, bile acids and blood lipid profiles, revealing a microbial mechanism for the lipid-lowering effect of LRS in hyperlipidemic rats.
36444019	3	61	theme	Dehydro-LCA	821:831	arg1	contents					804:811	the contents	800:811	the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats	800:881	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36444019	3	62	dep	acids	726:730	arg1	CA					733:734	CA	733:734	CA	733:734	Meanwhile LRS promoted the production of primary bile acids (CA, CDCA, β-MCA) and secondary bile acids (LCA, UDCA), and reduced the contents of TCA, Dehydro-LCA, isoLCA, LCA-3-S and THDCA in hyperlipidemic rats.
36943347	7	0	dep	identity	566:573	arg1	value					575:579	value	575:579	The average nucleotide identity value	543:579	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	7	1	theme	85.4	712:715	arg1	identity					566:573	The average nucleotide identity	543:573	The average nucleotide identity value	543:579	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	7	1	theme	85.4	712:715	arg1	%					732:732	85.4, 85.2 and 29.2 %	712:732	%	732:732	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	12	2	theme	parakribbianus	1079:1092	arg1	sp					1094:1095	The name Lacticaseibacillus parakribbianus sp	1051:1095	The name Lacticaseibacillus parakribbianus sp.	1051:1096	The name Lacticaseibacillus parakribbianus sp.
36943347	10	3	contain	contain	947:953	arg1	peptidoglycan					925:937	The cell-wall peptidoglycan	911:937	The cell-wall peptidoglycan	911:937	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
36943347	10	3	contain	contain	947:953	arg2	acid					975:978	meso-diaminopimelic acid	955:978	meso-diaminopimelic acid	955:978	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
36943347	8	4	theme	G+C	753:755	arg1	content					757:763	The G+C content	749:763	The G+C content of the genomic DNA	749:782	The G+C content of the genomic DNA was 61.6 mol%.
36943347	8	4	theme	G+C	753:755	arg1	%					796:796	61.6 mol%	788:796	61.6 mol%	788:796	The G+C content of the genomic DNA was 61.6 mol%.
36943347	2	5	theme	acid	91:94	arg1	bacterium					96:104	A lactic acid bacterium	82:104	A lactic acid bacterium isolated from pig faeces	82:129	A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach.
36943347	7	6	theme	amino	590:594	arg1	identity					601:608	average amino acid identity	582:608	average amino acid identity	582:608	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	10	7	theme	cell-wall	915:923	arg1	peptidoglycan					925:937	The cell-wall peptidoglycan	911:937	The cell-wall peptidoglycan	911:937	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
36943347	7	8	theme	acid	596:599	arg1	identity					601:608	average amino acid identity	582:608	average amino acid identity	582:608	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	1	9	theme	pig	60:62	arg1	farm					64:67	a pig farm	58:67	a pig farm	58:67	nov., isolated from a pig farm faeces dump.
36943347	4	10	theme	rRNA	286:289	arg1	sequence					296:303	the 16S rRNA gene sequence	278:303	the 16S rRNA gene sequence	278:303	Phylogenetic analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus.
36943347	5	11	theme	multi-locus	378:388	arg1	tree					399:402	The multi-locus sequence tree	374:402	The multi-locus sequence tree	374:402	The multi-locus sequence tree revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus kribbianus.
36943347	7	12	theme	genome-to-genome	615:630	arg1	distance					632:639	genome-to-genome distance	615:639	genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus,	615:705	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	4	13	theme	16S	282:284	arg1	rRNA					286:289	16S rRNA	282:289	the 16S rRNA gene sequence	278:303	Phylogenetic analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus.
36943347	1	14	attach	isolated	44:51	arg1	farm					64:67	a pig farm	58:67	a pig farm	58:67	nov., isolated from a pig farm faeces dump.
36943347	1	14	attach	isolated	44:51	arg2	nov.					38:41	nov.	38:41	nov.	38:41	nov., isolated from a pig farm faeces dump.
36943347	7	15	theme	average	582:588	arg1	identity					601:608	average amino acid identity	582:608	average amino acid identity	582:608	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	4	16	theme	sequence	296:303	arg1	analysis					266:273	Phylogenetic analysis	253:273	Phylogenetic analysis of the 16S rRNA gene sequence	253:303	Phylogenetic analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus.
36943347	0	17	theme	parakribbianus	19:32	arg1	sp					34:35	Lacticaseibacillus parakribbianus sp	0:35	Lacticaseibacillus parakribbianus sp.	0:36	Lacticaseibacillus parakribbianus sp.
36943347	2	18	theme	pig	120:122	arg1	faeces					124:129	pig faeces	120:129	pig faeces	120:129	A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach.
36943347	12	19	theme	Lacticaseibacillus	1060:1077	arg1	sp					1094:1095	The name Lacticaseibacillus parakribbianus sp	1051:1095	The name Lacticaseibacillus parakribbianus sp.	1051:1096	The name Lacticaseibacillus parakribbianus sp.
36943347	4	20	theme	gene	291:294	arg1	sequence					296:303	the 16S rRNA gene sequence	278:303	the 16S rRNA gene sequence	278:303	Phylogenetic analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus.
36943347	0	21	theme	Lacticaseibacillus	0:17	arg1	sp					34:35	Lacticaseibacillus parakribbianus sp	0:35	Lacticaseibacillus parakribbianus sp.	0:36	Lacticaseibacillus parakribbianus sp.
36943347	9	22	theme	major	803:807	arg1	diphosphatidylglycerol					827:848	diphosphatidylglycerol	827:848	diphosphatidylglycerol	827:848	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36943347	9	22	theme	major	803:807	arg1	lipids					815:820	The major polar lipids	799:820	The major polar lipids	799:820	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36943347	9	23	theme	polar	809:813	arg1	diphosphatidylglycerol					827:848	diphosphatidylglycerol	827:848	diphosphatidylglycerol	827:848	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36943347	9	23	theme	polar	809:813	arg1	lipids					815:820	The major polar lipids	799:820	The major polar lipids	799:820	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, aminophospholipids and phospholipids.
36943347	7	24	theme	average	547:553	arg1	identity					566:573	The average nucleotide identity	543:573	The average nucleotide identity value	543:579	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	7	24	theme	average	547:553	arg1	%					732:732	85.4, 85.2 and 29.2 %	712:732	%	732:732	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	4	25	theme	Phylogenetic	253:264	arg1	analysis					266:273	Phylogenetic analysis	253:273	Phylogenetic analysis of the 16S rRNA gene sequence	253:303	Phylogenetic analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus.
36943347	8	26	theme	genomic	772:778	arg1	DNA					780:782	the genomic DNA	768:782	the genomic DNA	768:782	The G+C content of the genomic DNA was 61.6 mol%.
36943347	7	27	theme	nucleotide	555:564	arg1	identity					566:573	The average nucleotide identity	543:573	The average nucleotide identity value	543:579	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	7	27	theme	nucleotide	555:564	arg1	%					732:732	85.4, 85.2 and 29.2 %	712:732	%	732:732	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	11	28	theme	novel	1036:1040	arg1	species					1042:1048	a novel species	1034:1048	a novel species	1034:1048	Thus, YH-lacS6T (=KCTC 21186T=JCM 34954T) represents a novel species.
36943347	2	29	theme	polyphasic	157:166	arg1	approach					168:175	a polyphasic approach	155:175	a polyphasic approach	155:175	A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach.
36943347	2	30	theme	lactic	84:89	arg1	bacterium					96:104	A lactic acid bacterium	82:104	A lactic acid bacterium isolated from pig faeces	82:129	A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach.
36943347	6	31	theme	fatty	502:506	arg1	acids					508:512	The main fatty acids	493:512	The main fatty acids	493:512	The main fatty acids were C16 : 0 and C18 : 1ω9c.
36943347	6	31	theme	fatty	502:506	arg1	C16 					519:522	C16 	519:522	C16 	519:522	The main fatty acids were C16 : 0 and C18 : 1ω9c.
36943347	6	32	theme	main	497:500	arg1	acids					508:512	The main fatty acids	493:512	The main fatty acids	493:512	The main fatty acids were C16 : 0 and C18 : 1ω9c.
36943347	6	32	theme	main	497:500	arg1	C16 					519:522	C16 	519:522	C16 	519:522	The main fatty acids were C16 : 0 and C18 : 1ω9c.
36943347	10	33	theme	meso-diaminopimelic	955:973	arg1	acid					975:978	meso-diaminopimelic acid	955:978	meso-diaminopimelic acid	955:978	The cell-wall peptidoglycan did not contain meso-diaminopimelic acid.
36943347	7	34	theme	29.2 	727:731	arg1	identity					566:573	The average nucleotide identity	543:573	The average nucleotide identity value	543:579	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	7	34	theme	29.2 	727:731	arg1	%					732:732	85.4, 85.2 and 29.2 %	712:732	%	732:732	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	4	35	theme	genus	348:352	arg1	Lacticaseibacillus					354:371	the genus Lacticaseibacillus	344:371	the genus Lacticaseibacillus	344:371	Phylogenetic analysis of the 16S rRNA gene sequence indicated that the isolate belonged to the genus Lacticaseibacillus.
36943347	8	36	theme	DNA	780:782	arg1	content					757:763	The G+C content	749:763	The G+C content of the genomic DNA	749:782	The G+C content of the genomic DNA was 61.6 mol%.
36943347	8	36	theme	DNA	780:782	arg1	%					796:796	61.6 mol%	788:796	61.6 mol%	788:796	The G+C content of the genomic DNA was 61.6 mol%.
36943347	7	37	theme	related	676:682	arg1	strain					684:689	its most closely related strain	659:689	its most closely related strain	659:689	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	7	37	theme	related	676:682	arg1	kribbianus					695:704	L. kribbianus	692:704	L. kribbianus	692:704	The average nucleotide identity value, average amino acid identity, and genome-to-genome distance for YH-lacS6T and its most closely related strain, L. kribbianus, were 85.4, 85.2 and 29.2 %, respectively.
36943347	11	38	theme	=KCTC	998:1002	arg1	34954T					1015:1020	=KCTC 21186T=JCM 34954T	998:1020	=KCTC 21186T=JCM 34954T	998:1020	Thus, YH-lacS6T (=KCTC 21186T=JCM 34954T) represents a novel species.
36943347	11	38	theme	=KCTC	998:1002	arg1	YH-lacS6T					987:995	YH-lacS6T	987:995	YH-lacS6T (=KCTC 21186T=JCM 34954T)	987:1021	Thus, YH-lacS6T (=KCTC 21186T=JCM 34954T) represents a novel species.
36943347	5	39	theme	adjacent	450:457	arg1	sub-cluster					438:448	a sub-cluster	436:448	a sub-cluster adjacent to Lacticaseibacillus kribbianus	436:490	The multi-locus sequence tree revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus kribbianus.
36943347	5	40	theme	sequence	390:397	arg1	tree					399:402	The multi-locus sequence tree	374:402	The multi-locus sequence tree	374:402	The multi-locus sequence tree revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus kribbianus.
36943347	11	41	theme	21186T=JCM	1004:1013	arg1	34954T					1015:1020	=KCTC 21186T=JCM 34954T	998:1020	=KCTC 21186T=JCM 34954T	998:1020	Thus, YH-lacS6T (=KCTC 21186T=JCM 34954T) represents a novel species.
36943347	11	41	theme	21186T=JCM	1004:1013	arg1	YH-lacS6T					987:995	YH-lacS6T	987:995	YH-lacS6T (=KCTC 21186T=JCM 34954T)	987:1021	Thus, YH-lacS6T (=KCTC 21186T=JCM 34954T) represents a novel species.
36943347	8	42	theme	61.6 mol	788:795	arg1	content					757:763	The G+C content	749:763	The G+C content of the genomic DNA	749:782	The G+C content of the genomic DNA was 61.6 mol%.
36943347	8	42	theme	61.6 mol	788:795	arg1	%					796:796	61.6 mol%	788:796	61.6 mol%	788:796	The G+C content of the genomic DNA was 61.6 mol%.
36943347	12	43	theme	name	1055:1058	arg1	sp					1094:1095	The name Lacticaseibacillus parakribbianus sp	1051:1095	The name Lacticaseibacillus parakribbianus sp.	1051:1096	The name Lacticaseibacillus parakribbianus sp.
36943347	2	44	attach	isolated	106:113	arg1	faeces					124:129	pig faeces	120:129	pig faeces	120:129	A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach.
36943347	2	44	attach	isolated	106:113	arg2	bacterium					96:104	A lactic acid bacterium	82:104	A lactic acid bacterium isolated from pig faeces	82:129	A lactic acid bacterium isolated from pig faeces was characterized using a polyphasic approach.
36943347	5	45	theme	Lacticaseibacillus	462:479	arg1	kribbianus					481:490	Lacticaseibacillus kribbianus	462:490	Lacticaseibacillus kribbianus	462:490	The multi-locus sequence tree revealed that the strain formed a sub-cluster adjacent to Lacticaseibacillus kribbianus.
36943347	3	46	dep	Gram-stain-positive	193:211	arg1	strain					182:187	The strain	178:187	The strain	178:187	The strain was Gram-stain-positive, rod-shaped, and facultative anaerobic.
36943347	3	46	dep	Gram-stain-positive	193:211	arg1	Gram-stain-positive					193:211	Gram-stain-positive	193:211	Gram-stain-positive	193:211	The strain was Gram-stain-positive, rod-shaped, and facultative anaerobic.
36943347	3	46	dep	Gram-stain-positive	193:211	arg1	anaerobic					242:250	anaerobic	242:250	anaerobic	242:250	The strain was Gram-stain-positive, rod-shaped, and facultative anaerobic.
36142831	0	0	theme	Oxidative	94:102	arg1	Stress					104:109	Oxidative Stress	94:109	Oxidative Stress	94:109	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	2	1	theme	DEAE-52	486:492	arg1	cellulose					494:502	DEAE-52 cellulose	486:502	DEAE-52 cellulose	486:502	LBP was extracted with water, and DEAE-52 cellulose was used for purification.
36142831	7	2	theme	rat	1217:1219	arg1	liver					1221:1225	rat liver	1217:1225	rat liver	1217:1225	Transmission electron microscopy showed that LBP and LBPT increased the density of mitochondria in rat liver.
36142831	6	3	from	decrease	1046:1053	arg1	ATP					1073:1075	ATP	1073:1075	ATP	1073:1075	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	6	3	from	decrease	1046:1053	arg1	content					1090:1096	glycogen content	1081:1096	glycogen content	1081:1096	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	6	3	from	decrease	1046:1053	arg1	glucose					1064:1070	serum glucose	1058:1070	serum glucose	1058:1070	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	1	4	theme	study	162:166	arg1	purpose					146:152	The purpose	142:152	The purpose of this study	142:166	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	8	5	theme	signaling	1437:1445	arg1	pathway					1447:1453	the AMPK/PGC-1α signaling pathway	1421:1453	the AMPK/PGC-1α signaling pathway	1421:1453	In addition, molecular experiments showed that LBP and LBPT could improve oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway.
36142831	1	6	theme	practical	349:357	arg1	products					359:366	practical products	349:366	practical products	349:366	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	7	7	theme	Transmission	1118:1129	arg1	microscopy					1140:1149	Transmission electron microscopy	1118:1149	Transmission electron microscopy	1118:1149	Transmission electron microscopy showed that LBP and LBPT increased the density of mitochondria in rat liver.
36142831	8	8	theme	oxidative	1302:1310	arg1	stress					1312:1317	oxidative stress	1302:1317	oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway	1302:1453	In addition, molecular experiments showed that LBP and LBPT could improve oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway.
36142831	4	9	theme	Lycium	672:677	arg1	LBPT					725:728	LBPT	725:728	LBPT	725:728	Lycium barbarum polysaccharide effervescent tablets (LBPT) were prepared by mixing LBP and an excipient.
36142831	4	9	theme	Lycium	672:677	arg1	tablets					716:722	Lycium barbarum polysaccharide effervescent tablets	672:722	Lycium barbarum polysaccharide effervescent tablets (LBPT)	672:729	Lycium barbarum polysaccharide effervescent tablets (LBPT) were prepared by mixing LBP and an excipient.
36142831	6	10	theme	serum	1058:1062	arg1	glucose					1064:1070	serum glucose	1058:1070	serum glucose	1058:1070	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	0	11	theme	Energy	115:120	arg1	Metabolism					122:131	Energy Metabolism	115:131	Energy Metabolism	115:131	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	5	12	theme	Animal	777:782	arg1	experiments					784:794	Animal experiments	777:794	Animal experiments	777:794	Animal experiments showed that LBP and LBPT significantly increased the exhaustive swimming time in rats.
36142831	3	13	theme	chemical	535:542	arg1	structure					544:552	chemical structure	535:552	chemical structure	535:552	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	1	14	contain	have	391:394	arg2	capsules					442:449	capsules	442:449	capsules	442:449	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	1	14	contain	have	391:394	arg1	patients					378:385	patients	378:385	patients who have difficulty swallowing conventional tablets or capsules	378:449	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	1	14	contain	have	391:394	arg2	difficulty					396:405	difficulty swallowing conventional tablets or capsules	396:449	difficulty	396:405	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	6	15	theme	lactic	947:952	arg1	acid					954:957	lactic acid	947:957	lactic acid	947:957	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	7	16	theme	mitochondria	1201:1212	arg1	density					1190:1196	the density	1186:1196	the density of mitochondria in rat liver	1186:1225	Transmission electron microscopy showed that LBP and LBPT increased the density of mitochondria in rat liver.
36142831	3	17	dep	transform	603:611	arg1	infrared					613:620	infrared	613:620	transform infrared spectroscopy (FI-IR) and ion chromatography (IC)	603:669	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	0	18	theme	Anti-Fatigue	0:11	arg1	Effects					13:19	Anti-Fatigue Effects	0:19	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets	0:78	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	6	19	theme	creatine	963:970	arg1	kinase					972:977	creatine kinase	963:977	creatine kinase	963:977	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	0	20	from	Metabolism	122:131	arg1	Rats					136:139	Rats	136:139	Rats	136:139	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	0	21	theme	Lycium	24:29	arg1	Polysaccharide					40:53	Lycium barbarum Polysaccharide	24:53	Lycium barbarum Polysaccharide	24:53	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	4	22	dep	Lycium	672:677	arg1	barbarum					679:686	barbarum	679:686	barbarum	679:686	Lycium barbarum polysaccharide effervescent tablets (LBPT) were prepared by mixing LBP and an excipient.
36142831	3	23	theme	monosaccharide	558:571	arg1	composition					573:583	monosaccharide composition	558:583	monosaccharide composition	558:583	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	1	24	theme	anti-fatigue	191:202	arg1	effect					204:209	the anti-fatigue effect	187:209	the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP)	187:257	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	3	25	theme	LBP	588:590	arg1	structure					544:552	chemical structure	535:552	chemical structure	535:552	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	3	25	theme	LBP	588:590	arg1	composition					573:583	monosaccharide composition	558:583	monosaccharide composition	558:583	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	2	26	used	used	508:511	arg2	cellulose					494:502	DEAE-52 cellulose	486:502	DEAE-52 cellulose	486:502	LBP was extracted with water, and DEAE-52 cellulose was used for purification.
36142831	4	27	theme	effervescent	703:714	arg1	LBPT					725:728	LBPT	725:728	LBPT	725:728	Lycium barbarum polysaccharide effervescent tablets (LBPT) were prepared by mixing LBP and an excipient.
36142831	4	27	theme	effervescent	703:714	arg1	tablets					716:722	Lycium barbarum polysaccharide effervescent tablets	672:722	Lycium barbarum polysaccharide effervescent tablets (LBPT)	672:729	Lycium barbarum polysaccharide effervescent tablets (LBPT) were prepared by mixing LBP and an excipient.
36142831	0	28	theme	Polysaccharide	40:53	arg1	Effects					13:19	Anti-Fatigue Effects	0:19	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets	0:78	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	1	29	theme	functional	286:295	arg1	tablet					323:328	a functional anti-fatigue effervescent tablet	284:328	a functional anti-fatigue effervescent tablet	284:328	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	8	30	theme	AMPK/PGC-1α	1425:1435	arg1	pathway					1447:1453	the AMPK/PGC-1α signaling pathway	1421:1453	the AMPK/PGC-1α signaling pathway	1421:1453	In addition, molecular experiments showed that LBP and LBPT could improve oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway.
36142831	5	31	theme	exhaustive	849:858	arg1	time					869:872	the exhaustive swimming time	845:872	the exhaustive swimming time in rats	845:880	Animal experiments showed that LBP and LBPT significantly increased the exhaustive swimming time in rats.
36142831	7	32	theme	electron	1131:1138	arg1	microscopy					1140:1149	Transmission electron microscopy	1118:1149	Transmission electron microscopy	1118:1149	Transmission electron microscopy showed that LBP and LBPT increased the density of mitochondria in rat liver.
36142831	1	33	theme	anti-fatigue	297:308	arg1	tablet					323:328	a functional anti-fatigue effervescent tablet	284:328	a functional anti-fatigue effervescent tablet	284:328	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	1	34	dep	Lycium	222:227	arg1	barbarum					229:236	barbarum	229:236	barbarum	229:236	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	8	35	theme	signaling	1366:1374	arg1	pathway					1376:1382	the Nrf2/HO-1 signaling pathway	1352:1382	the Nrf2/HO-1 signaling pathway	1352:1382	In addition, molecular experiments showed that LBP and LBPT could improve oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway.
36142831	5	36	theme	swimming	860:867	arg1	time					869:872	the exhaustive swimming time	845:872	the exhaustive swimming time in rats	845:880	Animal experiments showed that LBP and LBPT significantly increased the exhaustive swimming time in rats.
36142831	1	37	theme	natural	214:220	arg1	polysaccharide					238:251	natural Lycium barbarum polysaccharide	214:251	natural Lycium barbarum polysaccharide (LBP)	214:257	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	1	37	theme	natural	214:220	arg1	LBP					254:256	LBP	254:256	LBP	254:256	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	0	38	theme	Effervescent	59:70	arg1	Tablets					72:78	Effervescent Tablets	59:78	Effervescent Tablets	59:78	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	1	39	theme	effervescent	310:321	arg1	tablet					323:328	a functional anti-fatigue effervescent tablet	284:328	a functional anti-fatigue effervescent tablet	284:328	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	6	40	theme	muscle	1021:1026	arg1	capacity					1005:1012	the antioxidant capacity	989:1012	the antioxidant capacity of rat muscle	989:1026	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	3	41	theme	ion	647:649	arg1	chromatography					651:664	ion chromatography	647:664	ion chromatography (IC)	647:669	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	3	41	theme	ion	647:649	arg1	IC					667:668	IC	667:668	IC	667:668	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	6	42	theme	biochemical	905:915	arg1	markers					917:923	biochemical markers	905:923	biochemical markers	905:923	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	6	42	theme	biochemical	905:915	arg1	kinase					972:977	creatine kinase	963:977	creatine kinase	963:977	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	6	42	theme	biochemical	905:915	arg1	acid					954:957	lactic acid	947:957	lactic acid	947:957	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	1	43	theme	swallowing	407:416	arg1	tablets					431:437	swallowing conventional tablets	407:437	swallowing conventional tablets	407:437	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	7	44	from	density	1190:1196	arg1	liver					1221:1225	rat liver	1217:1225	rat liver	1217:1225	Transmission electron microscopy showed that LBP and LBPT increased the density of mitochondria in rat liver.
36142831	1	45	theme	conventional	418:429	arg1	tablets					431:437	swallowing conventional tablets	407:437	swallowing conventional tablets	407:437	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	0	46	dep	Lycium	24:29	arg1	barbarum					31:38	barbarum	31:38	barbarum	31:38	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	3	47	dep	structure	544:552	arg1	The					531:533	The	531:533	The	531:533	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	8	48	theme	Nrf2/HO-1	1356:1364	arg1	pathway					1376:1382	the Nrf2/HO-1 signaling pathway	1352:1382	the Nrf2/HO-1 signaling pathway	1352:1382	In addition, molecular experiments showed that LBP and LBPT could improve oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway.
36142831	1	49	theme	Lycium	222:227	arg1	polysaccharide					238:251	natural Lycium barbarum polysaccharide	214:251	natural Lycium barbarum polysaccharide (LBP)	214:257	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	1	49	theme	Lycium	222:227	arg1	LBP					254:256	LBP	254:256	LBP	254:256	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	8	50	theme	energy	1399:1404	arg1	metabolism					1406:1415	energy metabolism	1399:1415	energy metabolism	1399:1415	In addition, molecular experiments showed that LBP and LBPT could improve oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway.
36142831	6	51	theme	glycogen	1081:1088	arg1	content					1090:1096	glycogen content	1081:1096	glycogen content	1081:1096	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	1	52	dep	difficulty	396:405	arg1	tablets					431:437	swallowing conventional tablets	407:437	swallowing conventional tablets	407:437	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	6	53	from	markers	917:923	arg1	serum					932:936	rat serum	928:936	rat serum	928:936	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	0	54	from	Stress	104:109	arg1	Rats					136:139	Rats	136:139	Rats	136:139	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	8	55	theme	molecular	1241:1249	arg1	experiments					1251:1261	molecular experiments	1241:1261	molecular experiments	1241:1261	In addition, molecular experiments showed that LBP and LBPT could improve oxidative stress caused by exercise by regulating the Nrf2/HO-1 signaling pathway and regulating energy metabolism via the AMPK/PGC-1α signaling pathway.
36142831	4	56	theme	polysaccharide	688:701	arg1	LBPT					725:728	LBPT	725:728	LBPT	725:728	Lycium barbarum polysaccharide effervescent tablets (LBPT) were prepared by mixing LBP and an excipient.
36142831	4	56	theme	polysaccharide	688:701	arg1	tablets					716:722	Lycium barbarum polysaccharide effervescent tablets	672:722	Lycium barbarum polysaccharide effervescent tablets (LBPT)	672:729	Lycium barbarum polysaccharide effervescent tablets (LBPT) were prepared by mixing LBP and an excipient.
36142831	3	57	dep	Fourier	595:601	arg1	transform					603:611	transform	603:611	transform infrared spectroscopy (FI-IR) and ion chromatography (IC)	603:669	The chemical structure and monosaccharide composition of LBP by Fourier transform infrared spectroscopy (FI-IR) and ion chromatography (IC).
36142831	1	58	theme	polysaccharide	238:251	arg1	effect					204:209	the anti-fatigue effect	187:209	the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP)	187:257	The purpose of this study was to investigate the anti-fatigue effect of natural Lycium barbarum polysaccharide (LBP) during exercise, develop a functional anti-fatigue effervescent tablet by applying LBP to practical products, and help patients who have difficulty swallowing conventional tablets or capsules.
36142831	0	59	theme	Tablets	72:78	arg1	Effects					13:19	Anti-Fatigue Effects	0:19	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets	0:78	Anti-Fatigue Effects of Lycium barbarum Polysaccharide and Effervescent Tablets by Regulating Oxidative Stress and Energy Metabolism in Rats.
36142831	6	60	theme	rat	928:930	arg1	serum					932:936	rat serum	928:936	rat serum	928:936	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	5	61	from	time	869:872	arg1	rats					877:880	rats	877:880	rats	877:880	Animal experiments showed that LBP and LBPT significantly increased the exhaustive swimming time in rats.
36142831	6	62	theme	rat	1017:1019	arg1	muscle					1021:1026	rat muscle	1017:1026	rat muscle	1017:1026	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
36142831	6	63	theme	antioxidant	993:1003	arg1	capacity					1005:1012	the antioxidant capacity	989:1012	the antioxidant capacity of rat muscle	989:1026	LBP and LBPT improved biochemical markers in rat serum, such as lactic acid and creatine kinase, enhanced the antioxidant capacity of rat muscle, and reversed the decrease in serum glucose, ATP and glycogen content caused by exercise.
37191980	2	0	theme	basidiomycetous	112:126	arg1	yeast					128:132	the basidiomycetous yeast	108:132	the basidiomycetous yeast genus Cystofilobasidium	108:156	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	3	1	theme	other	467:471	arg1	strains					473:479	three other strains	461:479	three other strains isolated from basal ice collected in Norway	461:523	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	2	theme	genus	613:617	arg1	species					598:604	a novel species	590:604	a novel species	590:604	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	5	3	theme	1.7 	807:810	arg1	%					811:811	1.7 %-4.1 and 11.3 %-17.1 %	807:833	%	811:811	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	1	4	theme	basidiomycetous	49:63	arg1	species					71:77	a novel basidiomycetous yeast species	41:77	a novel basidiomycetous yeast species	41:77	nov., a novel basidiomycetous yeast species.
37191980	6	5	with	germination	1000:1010	arg1	basidia					1017:1023	basidia	1017:1023	basidia	1017:1023	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	3	6	theme	26S	348:350	arg1	gene					366:369	the 26S ribosomal RNA gene	344:369	the 26S ribosomal RNA gene	344:369	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	7	theme	Cystofilobasidium	639:655	arg1	sp					670:671	the name Cystofilobasidium josepaulonis sp	630:671	the name Cystofilobasidium josepaulonis sp	630:671	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	5	8	theme	D1/D2	853:857	arg1	domain					859:864	the D1/D2 domain	849:864	the D1/D2 domain	849:864	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	1	9	theme	yeast	65:69	arg1	species					71:77	a novel basidiomycetous yeast species	41:77	a novel basidiomycetous yeast species	41:77	nov., a novel basidiomycetous yeast species.
37191980	2	10	from	zone	228:231	arg1	province					245:252	Shandong province	236:252	Shandong province	236:252	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	2	10	from	zone	228:231	arg1	China					258:262	PR China	255:262	PR China	255:262	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	6	11	theme	 V8	969:971	arg1	agar					979:982	10 % V8 juice agar	965:982	10 % V8 juice agar	965:982	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	3	12	theme	domain	334:339	arg1	sequences					311:319	sequences	311:319	sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region	311:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	13	theme	josepaulonis	657:668	arg1	sp					670:671	the name Cystofilobasidium josepaulonis sp	630:671	the name Cystofilobasidium josepaulonis sp	630:671	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	14	theme	basal	495:499	arg1	ice					501:503	basal ice	495:503	basal ice	495:503	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	6	15	from	teliospores	919:929	arg1	agar					950:953	potato dextrose agar	934:953	potato dextrose agar (PDA)	934:959	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	6	15	from	teliospores	919:929	arg1	agar					979:982	10 % V8 juice agar	965:982	10 % V8 juice agar	965:982	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	6	15	from	teliospores	919:929	arg1	PDA					956:958	PDA	956:958	PDA	956:958	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	4	16	theme	strain	689:694	arg1	nov					674:676	nov	674:676	nov	674:676	nov. (holotype strain CGMCC 2.6672T) is proposed.
37191980	4	16	theme	strain	689:694	arg1	2.6672T					702:708	holotype strain CGMCC 2.6672T	680:708	holotype strain CGMCC 2.6672T	680:708	nov. (holotype strain CGMCC 2.6672T) is proposed.
37191980	2	17	dep	yeast	128:132	arg1	Cystofilobasidium					140:156	genus Cystofilobasidium	134:156	the basidiomycetous yeast genus Cystofilobasidium	108:156	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	0	18	theme	josepaulonis	18:29	arg1	sp					31:32	Cystofilobasidium josepaulonis sp	0:32	Cystofilobasidium josepaulonis sp.	0:33	Cystofilobasidium josepaulonis sp.
37191980	3	19	theme	name	634:637	arg1	sp					670:671	the name Cystofilobasidium josepaulonis sp	630:671	the name Cystofilobasidium josepaulonis sp	630:671	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	5	20	theme	-17.1 	827:832	arg1	%					833:833	1.7 %-4.1 and 11.3 %-17.1 %	807:833	%	833:833	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	0	21	theme	Cystofilobasidium	0:16	arg1	sp					31:32	Cystofilobasidium josepaulonis sp	0:32	Cystofilobasidium josepaulonis sp.	0:33	Cystofilobasidium josepaulonis sp.
37191980	3	22	dep	spacer	400:405	arg1	ITS					408:410	ITS	408:410	ITS	408:410	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	5	23	dep	%	811:811	arg1	mismatches					835:844	mismatches	835:844	1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region	807:883	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	4	24	theme	holotype	680:687	arg1	nov					674:676	nov	674:676	nov	674:676	nov. (holotype strain CGMCC 2.6672T) is proposed.
37191980	4	24	theme	holotype	680:687	arg1	2.6672T					702:708	holotype strain CGMCC 2.6672T	680:708	holotype strain CGMCC 2.6672T	680:708	nov. (holotype strain CGMCC 2.6672T) is proposed.
37191980	5	25	theme	genus	780:784	arg1	Cystofilobasidium					786:802	the genus Cystofilobasidium	776:802	the genus Cystofilobasidium	776:802	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	3	26	theme	ribosomal	352:360	arg1	gene					366:369	the 26S ribosomal RNA gene	344:369	the 26S ribosomal RNA gene	344:369	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	6	27	theme	teliospore	989:998	arg1	germination					1000:1010	teliospore germination	989:1010	teliospore germination with basidia	989:1023	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	3	28	theme	insect	540:545	arg1	gut					530:532	the gut	526:532	the gut of an insect and an alga	526:557	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	6	29	theme	potato	934:939	arg1	agar					950:953	potato dextrose agar	934:953	potato dextrose agar (PDA)	934:959	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	6	29	theme	potato	934:939	arg1	PDA					956:958	PDA	956:958	PDA	956:958	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	3	30	theme	RNA	362:364	arg1	gene					366:369	the 26S ribosomal RNA gene	344:369	the 26S ribosomal RNA gene	344:369	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	4	31	theme	CGMCC	696:700	arg1	nov					674:676	nov	674:676	nov	674:676	nov. (holotype strain CGMCC 2.6672T) is proposed.
37191980	4	31	theme	CGMCC	696:700	arg1	2.6672T					702:708	holotype strain CGMCC 2.6672T	680:708	holotype strain CGMCC 2.6672T	680:708	nov. (holotype strain CGMCC 2.6672T) is proposed.
37191980	2	32	theme	sediment	185:192	arg1	sample					194:199	a marine sediment sample	176:199	a marine sediment sample collected in an intertidal zone in Shandong province, PR China	176:262	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	6	33	theme	%	968:968	arg1	agar					979:982	10 % V8 juice agar	965:982	10 % V8 juice agar	965:982	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	3	34	theme	transcribed	388:398	arg1	spacer					400:405	the internal transcribed spacer	375:405	the internal transcribed spacer (ITS) region	375:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	2	35	theme	marine	178:183	arg1	sample					194:199	a marine sediment sample	176:199	a marine sediment sample collected in an intertidal zone in Shandong province, PR China	176:262	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	2	36	theme	Shandong	236:243	arg1	province					245:252	Shandong province	236:252	Shandong province	236:252	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	2	36	theme	Shandong	236:243	arg1	China					258:262	PR China	255:262	PR China	255:262	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	6	37	theme	dextrose	941:948	arg1	agar					950:953	potato dextrose agar	934:953	potato dextrose agar (PDA)	934:959	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	6	37	theme	dextrose	941:948	arg1	PDA					956:958	PDA	956:958	PDA	956:958	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	3	38	theme	spacer	400:405	arg1	region					413:418	the internal transcribed spacer (ITS) region	375:418	the internal transcribed spacer (ITS) region	375:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	5	39	theme	novel	728:732	arg1	species					734:740	The novel species	724:740	The novel species	724:740	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	5	40	theme	known	759:763	arg1	species					765:771	the known species	755:771	the known species of the genus Cystofilobasidium	755:802	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	3	41	attach	isolated	481:488	arg2	strains					473:479	three other strains	461:479	three other strains isolated from basal ice collected in Norway	461:523	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	41	attach	isolated	481:488	arg1	ice					501:503	basal ice	495:503	basal ice	495:503	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	1	42	dep	species	71:77	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel basidiomycetous yeast species.
37191980	3	43	theme	internal	379:386	arg1	spacer					400:405	the internal transcribed spacer	375:405	the internal transcribed spacer (ITS) region	375:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	2	44	theme	yeast	82:86	arg1	strain					88:93	A yeast strain	80:93	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium	80:156	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	2	45	theme	intertidal	217:226	arg1	zone					228:231	an intertidal zone	214:231	an intertidal zone in Shandong province, PR China	214:262	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	2	46	attach	isolated	162:169	arg2	strain					88:93	A yeast strain	80:93	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium	80:156	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	2	46	attach	isolated	162:169	arg1	sample					194:199	a marine sediment sample	176:199	a marine sediment sample collected in an intertidal zone in Shandong province, PR China	176:262	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	5	47	from	mismatches	835:844	arg1	region					878:883	ITS region	874:883	the ITS region	870:883	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	5	47	from	mismatches	835:844	arg1	domain					859:864	the D1/D2 domain	849:864	the D1/D2 domain	849:864	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	3	48	theme	alga	554:557	arg1	gut					530:532	the gut	526:532	the gut of an insect and an alga	526:557	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	49	theme	phylogenetic	280:291	arg1	analyses					293:300	phylogenetic analyses	280:300	phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region	280:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	6	50	theme	juice	973:977	arg1	agar					979:982	10 % V8 juice agar	965:982	10 % V8 juice agar	965:982	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	3	51	dep	Russia	572:577	arg1	represent					580:588	represent	580:588	represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp	580:671	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	52	theme	analyses	293:300	arg1	results					269:275	The results	265:275	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region	265:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	53	theme	novel	592:596	arg1	species					598:604	a novel species	590:604	a novel species	590:604	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	2	54	theme	PR	255:256	arg1	province					245:252	Shandong province	236:252	Shandong province	236:252	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	2	54	theme	PR	255:256	arg1	China					258:262	PR China	255:262	PR China	255:262	A yeast strain belonging to the basidiomycetous yeast genus Cystofilobasidium was isolated from a marine sediment sample collected in an intertidal zone in Shandong province, PR China.
37191980	5	55	theme	%	826:826	arg1	%					833:833	1.7 %-4.1 and 11.3 %-17.1 %	807:833	%	833:833	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	6	56	theme	10 	965:967	arg1	%					968:968	%	968:968	%	968:968	This species forms teliospores on potato dextrose agar (PDA) and 10 % V8 juice agar, but teliospore germination with basidia was not observed.
37191980	5	57	theme	Cystofilobasidium	786:802	arg1	species					765:771	the known species	755:771	the known species of the genus Cystofilobasidium	755:802	The novel species differs from the known species of the genus Cystofilobasidium by 1.7 %-4.1 and 11.3 %-17.1 % mismatches in the D1/D2 domain and the ITS region, respectively.
37191980	3	58	theme	region	413:418	arg1	sequences					311:319	sequences	311:319	sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region	311:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	59	theme	D1/D2	328:332	arg1	gene					366:369	the 26S ribosomal RNA gene	344:369	the 26S ribosomal RNA gene	344:369	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	59	theme	D1/D2	328:332	arg1	domain					334:339	the D1/D2 domain	324:339	the D1/D2 domain of the 26S ribosomal RNA gene	324:369	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	1	60	theme	novel	43:47	arg1	species					71:77	a novel basidiomycetous yeast species	41:77	a novel basidiomycetous yeast species	41:77	nov., a novel basidiomycetous yeast species.
37191980	3	61	theme	gene	366:369	arg1	gene					366:369	the 26S ribosomal RNA gene	344:369	the 26S ribosomal RNA gene	344:369	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	61	theme	gene	366:369	arg1	region					413:418	the internal transcribed spacer (ITS) region	375:418	the internal transcribed spacer (ITS) region	375:418	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
37191980	3	61	theme	gene	366:369	arg1	domain					334:339	the D1/D2 domain	324:339	the D1/D2 domain of the 26S ribosomal RNA gene	324:369	The results of phylogenetic analyses based on sequences of the D1/D2 domain of the 26S ribosomal RNA gene and the internal transcribed spacer (ITS) region indicate that this strain, together with three other strains isolated from basal ice collected in Norway, the gut of an insect and an alga collected in Russia, represent a novel species of the genus, for which the name Cystofilobasidium josepaulonis sp.
36567032	4	0	from	acids	539:543	arg1	plasma					588:593	the plasma	584:593	the plasma	584:593	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	0	from	acids	539:543	arg1	acids					602:606	fatty acids	596:606	fatty acids profile, TLR-4 and claudin-5 expressions in the brain	596:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	0	from	acids	539:543	arg1	TLR-4					617:621	TLR-4	617:621	TLR-4	617:621	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	0	from	acids	539:543	arg1	memory					674:679	social memory	667:679	social memory	667:679	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	0	from	acids	539:543	arg1	profile					608:614	profile	608:614	profile	608:614	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	8	1	theme	caprylic	1020:1027	arg1	acids					1100:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	4	2	from	memory	674:679	arg1	brain					656:660	the brain	652:660	the brain	652:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	8	3	theme	lignoceric	1062:1071	arg1	acids					1100:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	1	4	theme	cafeteria	307:315	arg1	diet					317:320	cafeteria diet	307:320	cafeteria diet	307:320	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	10	5	theme	CAF-fed	1253:1259	arg1	animals					1261:1267	CAF-fed animals	1253:1267	CAF-fed animals	1253:1267	In the social memory test, CAF-fed animals showed greater social interaction with no effect of n3.
36567032	4	6	theme	fatty	596:600	arg1	acids					602:606	fatty acids	596:606	fatty acids profile, TLR-4 and claudin-5 expressions in the brain	596:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	6	theme	fatty	596:600	arg1	TLR-4					617:621	TLR-4	617:621	TLR-4	617:621	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	6	theme	fatty	596:600	arg1	profile					608:614	profile	608:614	profile	608:614	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	11	7	theme	effect	1352:1357	arg1	lack					1341:1344	The lack	1337:1344	The lack of n3 effect	1337:1357	CONCLUSIONS The lack of n3 effect in some of the evaluated parameters may be due to the severity of the obesity caused by CAF.
36567032	1	8	theme	intestinal	223:232	arg1	microbiota					234:243	intestinal microbiota	223:243	intestinal microbiota	223:243	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	0	9	theme	acids	84:88	arg1	Impact					0:5	Impact	0:5	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota	0:75	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	9	theme	acids	84:88	arg1	levels					90:95	fatty acids levels	78:95	fatty acids levels	78:95	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	10	from	Impact	0:5	arg1	microbiota					66:75	the intestinal microbiota	51:75	the intestinal microbiota	51:75	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	4	11	dep	acids	602:606	arg1	acids					602:606	fatty acids	596:606	fatty acids profile, TLR-4 and claudin-5 expressions in the brain	596:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	11	dep	acids	602:606	arg1	TLR-4					617:621	TLR-4	617:621	TLR-4	617:621	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	11	dep	acids	602:606	arg1	expressions					637:647	claudin-5 expressions	627:647	claudin-5 expressions	627:647	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	11	dep	acids	602:606	arg1	profile					608:614	profile	608:614	profile	608:614	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	12	from	plasma	588:593	arg1	brain					656:660	the brain	652:660	the brain	652:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	13	theme	short-chain	521:531	arg1	acids					539:543	short-chain fatty acids	521:543	short-chain fatty acids (SCFA)	521:550	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	13	theme	short-chain	521:531	arg1	SCFA					546:549	SCFA	546:549	SCFA	546:549	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	0	14	theme	fatty	78:82	arg1	acids					84:88	fatty acids	78:88	fatty acids levels	78:95	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	9	15	theme	cerebral	1163:1170	arg1	cortex					1172:1177	the cerebral cortex	1159:1177	the cerebral cortex	1159:1177	CAF increased palmitic acid and TLR-4 expression in the cerebral cortex while decreasing claudin-5 in the hippocampus.
36567032	0	16	from	memory	135:140	arg1	rats					150:153	male rats	145:153	male rats	145:153	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	4	17	from	composition	508:518	arg1	plasma					588:593	the plasma	584:593	the plasma	584:593	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	17	from	composition	508:518	arg1	acids					602:606	fatty acids	596:606	fatty acids profile, TLR-4 and claudin-5 expressions in the brain	596:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	17	from	composition	508:518	arg1	TLR-4					617:621	TLR-4	617:621	TLR-4	617:621	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	17	from	composition	508:518	arg1	memory					674:679	social memory	667:679	social memory	667:679	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	17	from	composition	508:518	arg1	profile					608:614	profile	608:614	profile	608:614	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	8	18	theme	stearic	1040:1046	arg1	acids					1100:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	10	19	theme	memory	1240:1245	arg1	test					1247:1250	the social memory test	1229:1250	the social memory test	1229:1250	In the social memory test, CAF-fed animals showed greater social interaction with no effect of n3.
36567032	4	20	theme	social	667:672	arg1	memory					674:679	social memory	667:679	social memory	667:679	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	12	21	theme	LPS	1472:1474	arg1	levels					1476:1481	LPS levels	1472:1481	LPS levels	1472:1481	However, n3 reduced LPS levels, suggesting its ability to reverse endotoxemia.
36567032	2	22	theme	Male	346:349	arg1	rats					358:361	Male Wistar rats	346:361	Male Wistar rats	346:361	METHODS Male Wistar rats were fed with CAF for 20 weeks.
36567032	9	23	from	expression	1145:1154	arg1	cortex					1172:1177	the cerebral cortex	1159:1177	the cerebral cortex	1159:1177	CAF increased palmitic acid and TLR-4 expression in the cerebral cortex while decreasing claudin-5 in the hippocampus.
36567032	6	24	from	butyrate	879:886	arg1	rats					913:916	obese rats	907:916	obese rats	907:916	CAF increased SCFA plasma levels, but n3 reduced butyrate and isobutyrate in obese rats.
36567032	10	25	theme	social	1284:1289	arg1	interaction					1291:1301	greater social interaction	1276:1301	greater social interaction	1276:1301	In the social memory test, CAF-fed animals showed greater social interaction with no effect of n3.
36567032	3	26	theme	20th	457:460	arg1	week					462:465	the 16th and 20th week	444:465	week	462:465	Omega-3 (500 mg/kg/day) was supplemented between the 16th and 20th week.
36567032	8	27	theme	linoleic	1091:1098	arg1	acids					1100:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	5	28	theme	RESULTS	700:706	arg1	CAF					708:710	RESULTS CAF	700:710	RESULTS CAF	700:710	RESULTS CAF reduced colon length, crypts' depth, and microbiota diversity, while n3 increased the Firmicutes/Bacteroidetes ratio.
36567032	1	29	theme	-fed	327:330	arg1	rats					332:335	cafeteria diet (CAF)-fed rats	307:335	cafeteria diet (CAF)-fed rats	307:335	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	10	30	theme	n3	1321:1322	arg1	effect					1311:1316	no effect	1308:1316	no effect of n3	1308:1322	In the social memory test, CAF-fed animals showed greater social interaction with no effect of n3.
36567032	10	31	theme	greater	1276:1282	arg1	interaction					1291:1301	greater social interaction	1276:1301	greater social interaction	1276:1301	In the social memory test, CAF-fed animals showed greater social interaction with no effect of n3.
36567032	1	32	theme	fatty	246:250	arg1	profile					258:264	fatty acids profile	246:264	fatty acids profile	246:264	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	6	33	from	isobutyrate	892:902	arg1	rats					913:916	obese rats	907:916	obese rats	907:916	CAF increased SCFA plasma levels, but n3 reduced butyrate and isobutyrate in obese rats.
36567032	4	34	theme	claudin-5	627:635	arg1	expressions					637:647	claudin-5 expressions	627:647	claudin-5 expressions	627:647	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	5	35	theme	microbiota	753:762	arg1	diversity					764:772	microbiota diversity	753:772	microbiota diversity	753:772	RESULTS CAF reduced colon length, crypts' depth, and microbiota diversity, while n3 increased the Firmicutes/Bacteroidetes ratio.
36567032	0	36	theme	diet	20:23	arg1	Impact					0:5	Impact	0:5	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota	0:75	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	36	theme	diet	20:23	arg1	levels					90:95	fatty acids levels	78:95	fatty acids levels	78:95	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	36	theme	diet	20:23	arg1	markers					116:122	neuroinflammatory markers	98:122	neuroinflammatory markers	98:122	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	36	theme	diet	20:23	arg1	memory					135:140	social memory	128:140	social memory	128:140	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	1	37	theme	acids	252:256	arg1	profile					258:264	fatty acids profile	246:264	fatty acids profile	246:264	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	8	38	theme	cerebral	990:997	arg1	cortex					999:1004	the cerebral cortex	986:1004	the cerebral cortex	986:1004	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	6	39	theme	obese	907:911	arg1	rats					913:916	obese rats	907:916	obese rats	907:916	CAF increased SCFA plasma levels, but n3 reduced butyrate and isobutyrate in obese rats.
36567032	4	40	from	lipopolysaccharide	556:573	arg1	plasma					588:593	the plasma	584:593	the plasma	584:593	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	40	from	lipopolysaccharide	556:573	arg1	acids					602:606	fatty acids	596:606	fatty acids profile, TLR-4 and claudin-5 expressions in the brain	596:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	40	from	lipopolysaccharide	556:573	arg1	TLR-4					617:621	TLR-4	617:621	TLR-4	617:621	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	40	from	lipopolysaccharide	556:573	arg1	memory					674:679	social memory	667:679	social memory	667:679	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	40	from	lipopolysaccharide	556:573	arg1	profile					608:614	profile	608:614	profile	608:614	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	0	41	theme	cafeteria	10:18	arg1	diet					20:23	cafeteria diet	10:23	cafeteria diet	10:23	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	4	42	theme	intestinal	486:495	arg1	composition					508:518	intestinal microbiota composition	486:518	intestinal microbiota composition	486:518	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	0	43	theme	neuroinflammatory	98:114	arg1	Impact					0:5	Impact	0:5	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota	0:75	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	43	theme	neuroinflammatory	98:114	arg1	markers					116:122	neuroinflammatory markers	98:122	neuroinflammatory markers	98:122	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	1	44	theme	rats	332:335	arg1	profile					258:264	fatty acids profile	246:264	fatty acids profile	246:264	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	1	44	theme	rats	332:335	arg1	memory					297:302	social memory	290:302	social memory	290:302	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	1	44	theme	rats	332:335	arg1	microbiota					234:243	intestinal microbiota	223:243	intestinal microbiota	223:243	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	1	44	theme	rats	332:335	arg1	neuroinflammation					267:283	neuroinflammation	267:283	neuroinflammation	267:283	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	0	45	theme	n3	29:30	arg1	supplementation					32:46	n3 supplementation	29:46	n3 supplementation	29:46	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	46	theme	social	128:133	arg1	Impact					0:5	Impact	0:5	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota	0:75	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	46	theme	social	128:133	arg1	memory					135:140	social memory	128:140	social memory	128:140	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	8	47	theme	tricosanoic	1049:1059	arg1	acids					1100:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	4	48	theme	microbiota	497:506	arg1	composition					508:518	intestinal microbiota composition	486:518	intestinal microbiota composition	486:518	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	6	49	theme	plasma	849:854	arg1	levels					856:861	SCFA plasma levels	844:861	SCFA plasma levels	844:861	CAF increased SCFA plasma levels, but n3 reduced butyrate and isobutyrate in obese rats.
36567032	4	50	from	morphology	474:483	arg1	plasma					588:593	the plasma	584:593	the plasma	584:593	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	50	from	morphology	474:483	arg1	acids					602:606	fatty acids	596:606	fatty acids profile, TLR-4 and claudin-5 expressions in the brain	596:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	50	from	morphology	474:483	arg1	TLR-4					617:621	TLR-4	617:621	TLR-4	617:621	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	50	from	morphology	474:483	arg1	memory					674:679	social memory	667:679	social memory	667:679	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	50	from	morphology	474:483	arg1	profile					608:614	profile	608:614	profile	608:614	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	1	51	theme	omega-3	191:197	arg1	supplementation					204:218	omega-3 (n3) supplementation	191:218	omega-3 (n3) supplementation	191:218	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	1	52	dep	-fed	327:330	arg1	diet					317:320	cafeteria diet	307:320	cafeteria diet	307:320	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	4	53	theme	Colon	468:472	arg1	morphology					474:483	Colon morphology	468:483	Colon morphology	468:483	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	11	54	theme	obesity	1429:1435	arg1	severity					1413:1420	the severity	1409:1420	the severity of the obesity caused by CAF	1409:1449	CONCLUSIONS The lack of n3 effect in some of the evaluated parameters may be due to the severity of the obesity caused by CAF.
36567032	0	55	theme	supplementation	32:46	arg1	Impact					0:5	Impact	0:5	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota	0:75	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	55	theme	supplementation	32:46	arg1	levels					90:95	fatty acids levels	78:95	fatty acids levels	78:95	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	55	theme	supplementation	32:46	arg1	markers					116:122	neuroinflammatory markers	98:122	neuroinflammatory markers	98:122	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	0	55	theme	supplementation	32:46	arg1	memory					135:140	social memory	128:140	social memory	128:140	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	6	56	theme	SCFA	844:847	arg1	levels					856:861	SCFA plasma levels	844:861	SCFA plasma levels	844:861	CAF increased SCFA plasma levels, but n3 reduced butyrate and isobutyrate in obese rats.
36567032	7	57	theme	CAF-fed	940:946	arg1	rats					948:951	CAF-fed rats	940:951	CAF-fed rats	940:951	LPS was increased in CAF-fed rats, and n3 decreased its levels.
36567032	8	58	theme	myristoleic	1074:1084	arg1	acids					1100:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	1	59	theme	social	290:295	arg1	memory					297:302	social memory	290:302	social memory	290:302	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	9	60	theme	palmitic	1121:1128	arg1	acid					1130:1133	palmitic acid	1121:1133	palmitic acid	1121:1133	CAF increased palmitic acid and TLR-4 expression in the cerebral cortex while decreasing claudin-5 in the hippocampus.
36567032	1	61	from	effects	180:186	arg1	profile					258:264	fatty acids profile	246:264	fatty acids profile	246:264	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	1	61	from	effects	180:186	arg1	memory					297:302	social memory	290:302	social memory	290:302	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	1	61	from	effects	180:186	arg1	microbiota					234:243	intestinal microbiota	223:243	intestinal microbiota	223:243	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	1	61	from	effects	180:186	arg1	neuroinflammation					267:283	neuroinflammation	267:283	neuroinflammation	267:283	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	11	62	theme	n3	1349:1350	arg1	effect					1352:1357	n3 effect	1349:1357	n3 effect	1349:1357	CONCLUSIONS The lack of n3 effect in some of the evaluated parameters may be due to the severity of the obesity caused by CAF.
36567032	8	63	theme	palmitic	1030:1037	arg1	acids					1100:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids	1020:1104	In the cerebral cortex, n3 increased caprylic, palmitic, stearic, tricosanoic, lignoceric, myristoleic, and linoleic acids.
36567032	0	64	theme	male	145:148	arg1	rats					150:153	male rats	145:153	male rats	145:153	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	9	65	theme	acid	1130:1133	arg1	expression					1145:1154	palmitic acid and TLR-4 expression	1121:1154	palmitic acid and TLR-4 expression in the cerebral cortex	1121:1177	CAF increased palmitic acid and TLR-4 expression in the cerebral cortex while decreasing claudin-5 in the hippocampus.
36567032	5	66	theme	colon	720:724	arg1	length					726:731	colon length	720:731	colon length	720:731	RESULTS CAF reduced colon length, crypts' depth, and microbiota diversity, while n3 increased the Firmicutes/Bacteroidetes ratio.
36567032	10	67	theme	social	1233:1238	arg1	test					1247:1250	the social memory test	1229:1250	the social memory test	1229:1250	In the social memory test, CAF-fed animals showed greater social interaction with no effect of n3.
36567032	1	68	theme	n3	200:201	arg1	supplementation					204:218	omega-3 (n3) supplementation	191:218	omega-3 (n3) supplementation	191:218	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
36567032	0	69	theme	intestinal	55:64	arg1	microbiota					66:75	the intestinal microbiota	51:75	the intestinal microbiota	51:75	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	2	70	theme	Wistar	351:356	arg1	rats					358:361	Male Wistar rats	346:361	Male Wistar rats	346:361	METHODS Male Wistar rats were fed with CAF for 20 weeks.
36567032	0	71	from	markers	116:122	arg1	rats					150:153	male rats	145:153	male rats	145:153	Impact of cafeteria diet and n3 supplementation on the intestinal microbiota, fatty acids levels, neuroinflammatory markers and social memory in male rats.
36567032	11	72	theme	evaluated	1374:1382	arg1	parameters					1384:1393	the evaluated parameters	1370:1393	the evaluated parameters	1370:1393	CONCLUSIONS The lack of n3 effect in some of the evaluated parameters may be due to the severity of the obesity caused by CAF.
36567032	9	73	theme	TLR-4	1139:1143	arg1	expression					1145:1154	palmitic acid and TLR-4 expression	1121:1154	palmitic acid and TLR-4 expression in the cerebral cortex	1121:1177	CAF increased palmitic acid and TLR-4 expression in the cerebral cortex while decreasing claudin-5 in the hippocampus.
36567032	2	74	dep	METHODS	338:344	arg1	fed					368:370	fed	368:370	were fed with CAF for 20 weeks	363:392	METHODS Male Wistar rats were fed with CAF for 20 weeks.
36567032	4	75	theme	fatty	533:537	arg1	acids					539:543	short-chain fatty acids	521:543	short-chain fatty acids (SCFA)	521:550	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	4	75	theme	fatty	533:537	arg1	SCFA					546:549	SCFA	546:549	SCFA	546:549	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	3	76	theme	16th	448:451	arg1	week					462:465	the 16th and 20th week	444:465	week	462:465	Omega-3 (500 mg/kg/day) was supplemented between the 16th and 20th week.
36567032	4	77	from	acids	602:606	arg1	brain					656:660	the brain	652:660	the brain	652:660	Colon morphology, intestinal microbiota composition, short-chain fatty acids (SCFA) and lipopolysaccharide (LPS) in the plasma, fatty acids profile, TLR-4 and claudin-5 expressions in the brain, and social memory were investigated.
36567032	1	78	theme	supplementation	204:218	arg1	effects					180:186	the effects	176:186	the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats	176:335	OBJECTIVE To assess the effects of omega-3 (n3) supplementation on intestinal microbiota, fatty acids profile, neuroinflammation, and social memory of cafeteria diet (CAF)-fed rats.
35430601	7	0	dep	gene	1023:1026	arg1	diversity					1060:1068	diversity	1060:1068	diversity	1060:1068	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	7	0	dep	gene	1023:1026	arg1	richness					1047:1054	a higher microbial richness	1028:1054	a higher microbial richness	1028:1054	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	13	1	theme	shrimp	2616:2621	arg1	microbiota					2623:2632	the shrimp microbiota	2612:2632	the shrimp microbiota at farming conditions	2612:2654	Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions.
35430601	13	2	theme	beneficial	2588:2597	arg1	microbes					2599:2606	beneficial microbes	2588:2606	beneficial microbes for the shrimp microbiota at farming conditions	2588:2654	Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions.
35430601	1	3	theme	cultured	177:184	arg1	shrimps					186:192	cultured shrimps	177:192	cultured shrimps	177:192	Prebiotics and probiotics have shown a number of beneficial impacts preventing diseases in cultured shrimps.
35430601	12	4	located	found	2293:2297	arg1	AG10					2380:2383	AG10	2380:2383	AG10	2380:2383	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	12	4	located	found	2293:2297	arg2	delbrueckii					2276:2286	Lactobacillus delbrueckii	2262:2286	Lactobacillus delbrueckii	2262:2286	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	12	4	located	found	2293:2297	arg1	shrimps					2302:2308	shrimps	2302:2308	shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis	2302:2375	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	12	4	located	found	2293:2297	arg2	lactis					2251:2256	Lactobacillus lactis	2237:2256	Lactobacillus lactis	2237:2256	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	13	5	from	conditions	2645:2654	arg1	microbiota					2623:2632	the shrimp microbiota	2612:2632	the shrimp microbiota at farming conditions	2612:2654	Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions.
35430601	7	6	theme	higher	1030:1035	arg1	richness					1047:1054	a higher microbial richness	1028:1054	a higher microbial richness	1028:1054	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	6	7	theme	growth	917:922	arg1	performance					924:934	growth performance	917:934	growth performance	917:934	At the same time, no effect on growth performance was observed in AG10.
35430601	10	8	theme	agavin	2079:2084	arg1	increases					2100:2108	agavin concentration increases	2079:2108	agavin concentration increases in the hepatopancreas	2079:2130	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	10	8	theme	agavin	2079:2084	arg1	increase					2048:2055	an increase	2045:2055	an increase in their abundance	2045:2074	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	8	9	theme	diversity	1278:1286	arg1	analysis					1288:1295	The beta diversity analysis	1269:1295	The beta diversity analysis	1269:1295	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	7	10	theme	gene	1023:1026	arg1	V3-V4 regions					993:1005	the V3-V4 regions	989:1005	the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine	989:1104	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	11	11	theme	beneficial	2204:2213	arg1	microbes					2215:2222	the 42 beneficial microbes	2197:2222	the 42 beneficial microbes	2197:2222	In addition, we also sequenced the DNA of agavin and found 9 of the 42 beneficial microbes.
35430601	9	12	dep	increased	1681:1689	arg1	both					1896:1899	both	1896:1899	both	1896:1899	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	5	13	theme	protein	809:815	arg1	ratio					828:832	protein efficiency ratio	809:832	protein efficiency ratio	809:832	After 28 days-trial, the feed conversion ratio, total feed ingested, and protein efficiency ratio was significantly improved on animals fed with AG2.
35430601	9	14	theme	16S	1651:1653	arg1	data					1655:1658	our 16S data	1647:1658	our 16S data	1647:1658	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	7	15	theme	16S	1014:1016	arg1	gene					1023:1026	the 16S rRNA gene a higher microbial richness and diversity	1010:1068	the 16S rRNA gene a higher microbial richness and diversity	1010:1068	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	6	16	theme	same	893:896	arg1	time					898:901	the same time	889:901	the same time	889:901	At the same time, no effect on growth performance was observed in AG10.
35430601	4	17	theme	agavin-free	707:717	arg1	BD					731:732	BD	731:732	BD	731:732	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	4	17	theme	agavin-free	707:717	arg1	diet					725:728	an agavin-free basal diet	704:728	an agavin-free basal diet (BD)	704:733	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	8	18	theme	agavin	1406:1411	arg1	supplementation					1413:1427	agavin supplementation	1406:1427	agavin supplementation	1406:1427	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	2	19	theme	microbiota	260:269	arg1	biodiversity					271:282	microbiota biodiversity	260:282	microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS)	260:369	Complex soluble carbohydrates are considered ideal for fostering microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS).
35430601	1	20	theme	impacts	146:152	arg1	number					125:130	a number	123:130	a number of beneficial impacts preventing diseases in cultured shrimps	123:192	Prebiotics and probiotics have shown a number of beneficial impacts preventing diseases in cultured shrimps.
35430601	12	21	dep	AG2	2332:2334	arg1	both					2327:2330	both	2327:2330	both	2327:2330	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	11	22	theme	agavin	2175:2180	arg1	DNA					2168:2170	the DNA	2164:2170	the DNA of agavin	2164:2180	In addition, we also sequenced the DNA of agavin and found 9 of the 42 beneficial microbes.
35430601	10	23	theme	microbes	1980:1987	arg1	abundance					1953:1961	the abundance	1949:1961	the abundance of 42 beneficial microbes	1949:1987	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	10	23	theme	microbes	1980:1987	arg1	meta-community					2022:2035	a single microbial community "meta-community	1992:2035	a single microbial community "meta-community	1992:2035	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	7	24	theme	AG10	1152:1155	arg1	diet					1157:1160	the AG10 diet	1148:1160	the AG10 diet	1148:1160	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	8	25	theme	deterministic	1452:1464	arg1	effect					1466:1471	a more substantial deterministic effect	1433:1471	a more substantial deterministic effect on the microbiota of hepatopancreas	1433:1507	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	8	26	theme	beta	1273:1276	arg1	analysis					1288:1295	The beta diversity analysis	1269:1295	The beta diversity analysis	1269:1295	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	7	27	dep	richness	1214:1221	arg1	both					1238:1241	both	1238:1241	both	1238:1241	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	8	28	theme	hepatopancreas	1494:1507	arg1	microbiota					1480:1489	the microbiota	1476:1489	the microbiota of hepatopancreas	1476:1507	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	3	29	theme	growth	394:399	arg1	performance					401:411	growth performance	394:411	growth performance	394:411	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	8	30	theme	significant	1312:1322	arg1	clustering					1335:1344	a clear significant microbiota clustering	1304:1344	a clear significant microbiota clustering by agavin diets	1304:1360	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	0	31	theme	beneficial	15:24	arg1	microbes					26:33	beneficial microbes	15:33	beneficial microbes	15:33	Agavin induces beneficial microbes in the shrimp microbiota under farming conditions.
35430601	3	32	theme	the white	443:451	arg1	shrimp					453:458	the white shrimp	443:458	the white shrimp	443:458	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	4	33	theme	shrimp	604:609	arg1	farm					611:614	a shrimp farm	602:614	a shrimp farm	602:614	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	10	34	from	increase	2048:2055	arg1	abundance					2066:2074	their abundance	2060:2074	their abundance	2060:2074	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	5	35	theme	conversion	766:775	arg1	ratio					777:781	the feed conversion ratio	757:781	the feed conversion ratio	757:781	After 28 days-trial, the feed conversion ratio, total feed ingested, and protein efficiency ratio was significantly improved on animals fed with AG2.
35430601	8	36	theme	agavin	1349:1354	arg1	diets					1356:1360	agavin diets	1349:1360	agavin diets	1349:1360	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	13	37	theme	farming	2637:2643	arg1	conditions					2645:2654	farming conditions	2637:2654	farming conditions	2637:2654	Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions.
35430601	10	38	theme	microbial	2001:2009	arg1	abundance					1953:1961	the abundance	1949:1961	the abundance of 42 beneficial microbes	1949:1987	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	10	38	theme	microbial	2001:2009	arg1	meta-community					2022:2035	a single microbial community "meta-community	1992:2035	a single microbial community "meta-community	1992:2035	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	4	39	theme	agavin	634:639	arg1	effect					624:629	the effect	620:629	the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels	620:685	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	2	40	theme	Complex	195:201	arg1	carbohydrates					211:223	Complex soluble carbohydrates	195:223	Complex soluble carbohydrates	195:223	Complex soluble carbohydrates are considered ideal for fostering microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS).
35430601	9	41	dep	literature	1548:1557	arg1	search					1562:1567	search	1562:1567	to search beneficial microbes for shrimp's health	1559:1607	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	9	41	dep	literature	1548:1557	arg1	found					1613:1617	found	1613:1617	found sequences for 42 species in our 16S data	1613:1658	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	8	42	from	effect	1466:1471	arg1	microbiota					1480:1489	the microbiota	1476:1489	the microbiota of hepatopancreas	1476:1507	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	9	43	theme	Streptococcus	1828:1840	arg1	thermophiles					1842:1853	Streptococcus thermophiles th1435	1828:1860	Streptococcus thermophiles th1435 in the hepatopancreas of the AG2	1828:1893	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	3	44	theme	farming	549:555	arg1	conditions					557:566	farming conditions	549:566	farming conditions	549:566	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	9	45	from	hepatopancreas	1767:1780	arg1	hepatopancreas					1869:1882	the hepatopancreas	1865:1882	the hepatopancreas of the AG2	1865:1893	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	7	46	theme	microbial	1037:1045	arg1	richness					1047:1054	a higher microbial richness	1028:1054	a higher microbial richness	1028:1054	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	5	47	theme	efficiency	817:826	arg1	ratio					828:832	protein efficiency ratio	809:832	protein efficiency ratio	809:832	After 28 days-trial, the feed conversion ratio, total feed ingested, and protein efficiency ratio was significantly improved on animals fed with AG2.
35430601	0	48	theme	shrimp	42:47	arg1	microbiota					49:58	the shrimp microbiota	38:58	the shrimp microbiota	38:58	Agavin induces beneficial microbes in the shrimp microbiota under farming conditions.
35430601	9	49	from	thermophiles	1842:1853	arg1	hepatopancreas					1869:1882	the hepatopancreas	1865:1882	the hepatopancreas of the AG2	1865:1893	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	10	50	from	increases	2100:2108	arg1	hepatopancreas					2117:2130	the hepatopancreas	2113:2130	the hepatopancreas	2113:2130	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	13	51	theme	microbes	2599:2606	arg1	increase					2576:2583	an increase	2573:2583	an increase of beneficial microbes for the shrimp microbiota at farming conditions	2573:2654	Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions.
35430601	11	52	theme	microbes	2215:2222	arg1	microbes					2215:2222	the 42 beneficial microbes	2197:2222	the 42 beneficial microbes	2197:2222	In addition, we also sequenced the DNA of agavin and found 9 of the 42 beneficial microbes.
35430601	11	52	theme	microbes	2215:2222	arg1	9					2192:2192	9	2192:2192	9	2192:2192	In addition, we also sequenced the DNA of agavin and found 9 of the 42 beneficial microbes.
35430601	4	53	theme	%	671:671	arg1	levels					680:685	2% (AG2) or 10% (AG10) levels	657:685	2% (AG2) or 10% (AG10) levels	657:685	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	7	54	theme	rRNA	1018:1021	arg1	gene					1023:1026	the 16S rRNA gene a higher microbial richness and diversity	1010:1068	the 16S rRNA gene a higher microbial richness and diversity	1010:1068	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	7	55	located	found	1110:1114	arg1	animals					1130:1136	those animals	1124:1136	those animals receiving the AG10 diet	1124:1160	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	7	55	located	found	1110:1114	arg2	V3-V4 regions					993:1005	the V3-V4 regions	989:1005	the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine	989:1104	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	14	56	theme	shrimp	2702:2707	arg1	prebiotic					2709:2717	a shrimp prebiotic	2700:2717	a shrimp prebiotic	2700:2717	Our study provides the first evidence that a shrimp prebiotic may selectively modify the microbiota in an organ-dependent effect.
35430601	8	57	contain	had	1429:1431	arg2	effect					1466:1471	a more substantial deterministic effect	1433:1471	a more substantial deterministic effect on the microbiota of hepatopancreas	1433:1507	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	8	57	contain	had	1429:1431	arg1	supplementation					1413:1427	agavin supplementation	1406:1427	agavin supplementation	1406:1427	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	10	58	theme	concentration	2086:2098	arg1	increases					2100:2108	agavin concentration increases	2079:2108	agavin concentration increases in the hepatopancreas	2079:2130	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	10	58	theme	concentration	2086:2098	arg1	increase					2048:2055	an increase	2045:2055	an increase in their abundance	2045:2074	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	14	59	theme	first	2680:2684	arg1	evidence					2686:2693	the first evidence that a shrimp prebiotic may selectively modify the microbiota in an organ-dependent effect	2676:2784	the first evidence that a shrimp prebiotic may selectively modify the microbiota in an organ-dependent effect	2676:2784	Our study provides the first evidence that a shrimp prebiotic may selectively modify the microbiota in an organ-dependent effect.
35430601	14	60	theme	organ-dependent	2763:2777	arg1	effect					2779:2784	an organ-dependent effect	2760:2784	an organ-dependent effect	2760:2784	Our study provides the first evidence that a shrimp prebiotic may selectively modify the microbiota in an organ-dependent effect.
35430601	12	61	dep	agavin	2319:2324	arg1	AG10					2340:2343	AG10	2340:2343	AG10	2340:2343	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	12	61	dep	agavin	2319:2324	arg1	AG2					2332:2334	AG2	2332:2334	AG2	2332:2334	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	2	62	theme	fermentable	287:297	arg1	oligosaccharides					299:314	fermentable oligosaccharides	287:314	fermentable oligosaccharides	287:314	Complex soluble carbohydrates are considered ideal for fostering microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS).
35430601	2	62	theme	fermentable	287:297	arg1	FODMAPS					362:368	FODMAPS	362:368	FODMAPS	362:368	Complex soluble carbohydrates are considered ideal for fostering microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS).
35430601	0	63	theme	farming	66:72	arg1	conditions					74:83	farming conditions	66:83	farming conditions	66:83	Agavin induces beneficial microbes in the shrimp microbiota under farming conditions.
35430601	12	64	dep	absent	2399:2404	arg1	diet					2413:2416	the BD diet	2406:2416	the BD diet	2406:2416	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	12	64	dep	absent	2399:2404	arg1	they					2389:2392	they	2389:2392	they	2389:2392	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	1	65	theme	beneficial	135:144	arg1	impacts					146:152	beneficial impacts	135:152	beneficial impacts preventing diseases in cultured shrimps	135:192	Prebiotics and probiotics have shown a number of beneficial impacts preventing diseases in cultured shrimps.
35430601	9	66	theme	Rodopseudomonas	1798:1812	arg1	palustris					1814:1822	the AG10 and Rodopseudomonas palustris	1785:1822	palustris	1814:1822	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	13	67	theme	agavin	2531:2536	arg1	supplementation					2538:2552	agavin supplementation	2531:2552	agavin supplementation	2531:2552	Our work provides evidence that agavin supplementation is associated with an increase of beneficial microbes for the shrimp microbiota at farming conditions.
35430601	8	68	theme	substantial	1440:1450	arg1	effect					1466:1471	a more substantial deterministic effect	1433:1471	a more substantial deterministic effect on the microbiota of hepatopancreas	1433:1507	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	3	69	dep	performance	401:411	arg1	the					390:392	the	390:392	the	390:392	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	9	70	theme	AG2	1891:1893	arg1	hepatopancreas					1869:1882	the hepatopancreas	1865:1882	the hepatopancreas of the AG2	1865:1893	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	10	71	theme	beneficial	1969:1978	arg1	microbes					1980:1987	42 beneficial microbes	1966:1987	42 beneficial microbes	1966:1987	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	7	72	contain	had	1198:1200	arg2	richness					1214:1221	a decreased richness	1202:1221	a decreased richness	1202:1221	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	7	72	contain	had	1198:1200	arg2	diets					1243:1247	diets	1243:1247	diets	1243:1247	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	7	72	contain	had	1198:1200	arg2	diversity					1227:1235	diversity	1227:1235	diversity	1227:1235	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	7	72	contain	had	1198:1200	arg1	those					1169:1173	those	1169:1173	those	1169:1173	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	4	73	theme	%	658:658	arg1	levels					680:685	2% (AG2) or 10% (AG10) levels	657:685	2% (AG2) or 10% (AG10) levels	657:685	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	9	74	theme	palustris	1814:1822	arg1	thermophiles					1842:1853	Streptococcus thermophiles th1435	1828:1860	Streptococcus thermophiles th1435 in the hepatopancreas of the AG2	1828:1893	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	9	74	theme	palustris	1814:1822	arg1	hepatopancreas					1767:1780	the hepatopancreas	1763:1780	the hepatopancreas of the AG10 and Rodopseudomonas palustris	1763:1822	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	6	75	located	observed	940:947	arg1	AG10					952:955	AG10	952:955	AG10	952:955	At the same time, no effect on growth performance was observed in AG10.
35430601	6	75	located	observed	940:947	arg1	time					898:901	the same time	889:901	the same time	889:901	At the same time, no effect on growth performance was observed in AG10.
35430601	6	75	located	observed	940:947	arg2	effect					907:912	no effect	904:912	no effect on growth performance	904:934	At the same time, no effect on growth performance was observed in AG10.
35430601	3	76	theme	dietary	487:493	arg1	intervention					495:506	dietary intervention	487:506	dietary intervention	487:506	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	3	77	theme	microbiota	417:426	arg1	composition					428:438	microbiota composition	417:438	microbiota composition	417:438	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	4	78	theme	basal	719:723	arg1	BD					731:732	BD	731:732	BD	731:732	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	4	78	theme	basal	719:723	arg1	diet					725:728	an agavin-free basal diet	704:728	an agavin-free basal diet (BD)	704:733	Adult L. vannamei were raised at a shrimp farm and the effect of agavin supplemented at 2% (AG2) or 10% (AG10) levels were compared to an agavin-free basal diet (BD).
35430601	5	79	theme	total	784:788	arg1	feed					790:793	total feed	784:793	total feed ingested	784:802	After 28 days-trial, the feed conversion ratio, total feed ingested, and protein efficiency ratio was significantly improved on animals fed with AG2.
35430601	12	80	theme	Lysinibacillus	2351:2364	arg1	fusiformis					2366:2375	Lysinibacillus fusiformis	2351:2375	Lysinibacillus fusiformis	2351:2375	From those, Lactobacillus lactis and Lactobacillus delbrueckii were found in shrimps fed with agavin (both AG2 and AG10), and Lysinibacillus fusiformis in AG10 and they were absent the BD diet, suggesting these three species could be introduced with the agavin to the diet.
35430601	6	81	from	effect	907:912	arg1	performance					924:934	growth performance	917:934	growth performance	917:934	At the same time, no effect on growth performance was observed in AG10.
35430601	3	82	theme	shrimp	453:458	arg1	performance					401:411	growth performance	394:411	growth performance	394:411	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	3	82	theme	shrimp	453:458	arg1	composition					428:438	microbiota composition	417:438	microbiota composition	417:438	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	8	83	theme	clear	1306:1310	arg1	clustering					1335:1344	a clear significant microbiota clustering	1304:1344	a clear significant microbiota clustering by agavin diets	1304:1360	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	5	84	theme	feed	761:764	arg1	ratio					777:781	the feed conversion ratio	757:781	the feed conversion ratio	757:781	After 28 days-trial, the feed conversion ratio, total feed ingested, and protein efficiency ratio was significantly improved on animals fed with AG2.
35430601	7	85	theme	decreased	1204:1212	arg1	richness					1214:1221	a decreased richness	1202:1221	a decreased richness	1202:1221	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	9	86	theme	AG10	1789:1792	arg1	palustris					1814:1822	the AG10 and Rodopseudomonas palustris	1785:1822	palustris	1814:1822	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	8	87	theme	microbiota	1324:1333	arg1	clustering					1335:1344	a clear significant microbiota clustering	1304:1344	a clear significant microbiota clustering by agavin diets	1304:1360	The beta diversity analysis showed a clear significant microbiota clustering by agavin diets only in the hepatopancreas, suggesting that agavin supplementation had a more substantial deterministic effect on the microbiota of hepatopancreas than on the intestine.
35430601	10	88	theme	community	2011:2019	arg1	abundance					1953:1961	the abundance	1949:1961	the abundance of 42 beneficial microbes	1949:1987	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	10	88	theme	community	2011:2019	arg1	meta-community					2022:2035	a single microbial community "meta-community	1992:2035	a single microbial community "meta-community	1992:2035	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	2	89	theme	soluble	203:209	arg1	carbohydrates					211:223	Complex soluble carbohydrates	195:223	Complex soluble carbohydrates	195:223	Complex soluble carbohydrates are considered ideal for fostering microbiota biodiversity by fermentable oligosaccharides, disaccharides, monosaccharides, and polyols (FODMAPS).
35430601	3	90	theme	FODMAP	526:531	arg1	prebiotic					533:541	a FODMAP prebiotic	524:541	a FODMAP prebiotic under farming conditions	524:566	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	3	90	theme	FODMAP	526:531	arg1	agavin					514:519	agavin	514:519	agavin	514:519	Here we evaluated the growth performance and microbiota composition of the white shrimp Litopenaeus vannamei after dietary intervention using agavin as a FODMAP prebiotic under farming conditions.
35430601	9	91	theme	beneficial	1569:1578	arg1	microbes					1580:1587	beneficial microbes	1569:1587	beneficial microbes for shrimp's health	1569:1607	We analyzed the literature to search beneficial microbes for shrimp's health and found sequences for 42 species in our 16S data, being significantly increased Lactobacillus pentosus, Pseudomonas putida and Pseudomonas synxantha in the hepatopancreas of the AG10 and Rodopseudomonas palustris and Streptococcus thermophiles th1435 in the hepatopancreas of the AG2, both compared to BD.
35430601	7	92	theme	AG2	1189:1191	arg1	diet					1193:1196	the AG2 diet	1185:1196	the AG2 diet	1185:1196	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	10	93	theme	single	1994:1999	arg1	abundance					1953:1961	the abundance	1949:1961	the abundance of 42 beneficial microbes	1949:1987	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	10	93	theme	single	1994:1999	arg1	meta-community					2022:2035	a single microbial community "meta-community	1992:2035	a single microbial community "meta-community	1992:2035	Interestingly, when we analyzed the abundance of 42 beneficial microbes as a single microbial community "meta-community," found an increase in their abundance as agavin concentration increases in the hepatopancreas.
35430601	7	94	from	V3-V4 regions	993:1005	arg1	intestine					1096:1104	intestine	1096:1104	intestine	1096:1104	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
35430601	7	94	from	V3-V4 regions	993:1005	arg1	hepatopancreas					1077:1090	the hepatopancreas	1073:1090	the hepatopancreas	1073:1090	Surprisingly, after sequencing the V3-V4 regions of the 16S rRNA gene a higher microbial richness and diversity in the hepatopancreas and intestine was found only in those animals receiving the AG10 diet, while those receiving the AG2 diet had a decreased richness and diversity, both diets compared to the BD.
37355085	15	0	theme	treated	3178:3184	arg1	group					3186:3190	LPS treated group	3174:3190	LPS treated group	3174:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	16	1	theme	compounds	3330:3338	arg1	source					3313:3318	potential source	3303:3318	potential source of active compounds with antiproliferative and anti-inflammatory properties	3303:3394	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	16	1	theme	compounds	3330:3338	arg1	species					3251:3257	the Hedychium species	3237:3257	the Hedychium species traditionally used in therapeutics	3237:3292	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	3	2	theme	Hedychium	759:767	arg1	H.					778:779	H.	778:779	H.	778:779	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	2	theme	Hedychium	759:767	arg1	H.					792:793	H.	792:793	H.	792:793	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	2	theme	Hedychium	759:767	arg1	species					769:775	four Hedychium species	754:775	four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	754:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	2	theme	Hedychium	759:767	arg1	H.					824:825	H.	824:825	H.	824:825	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	2	theme	Hedychium	759:767	arg1	H.					809:810	H.	809:810	H.	809:810	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	13	3	theme	IC50	2529:2532	arg1	value					2534:2538	an IC50 value	2526:2538	an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL	2526:2583	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	15	4	theme	murine	2911:2916	arg1	macrophages					2918:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	16	5	theme	antiproliferative	3345:3361	arg1	properties					3385:3394	antiproliferative and anti-inflammatory properties	3345:3394	antiproliferative and anti-inflammatory properties	3345:3394	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	3	6	from	activities	713:722	arg1	H.					778:779	H.	778:779	H.	778:779	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	6	from	activities	713:722	arg1	H.					792:793	H.	792:793	H.	792:793	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	6	from	activities	713:722	arg1	species					769:775	four Hedychium species	754:775	four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	754:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	6	from	activities	713:722	arg1	H.					824:825	H.	824:825	H.	824:825	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	6	from	activities	713:722	arg1	H.					809:810	H.	809:810	H.	809:810	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	7	theme	current	597:603	arg1	research					605:612	The current research	593:612	THE STUDY The current research	583:612	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	9	8	theme	ELISA	1734:1738	arg1	technique					1740:1748	the ELISA technique	1730:1748	the ELISA technique	1730:1748	Furthermore, the levels of prostaglandin (PGE2) and pro-inflammatory cytokines was assessed using the ELISA technique.
37355085	15	9	theme	RAW	2901:2903	arg1	macrophages					2918:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	5	10	dep	analysis	1085:1092	arg1	K-means					1118:1124	K-means	1118:1124	K-means	1118:1124	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	5	10	dep	analysis	1085:1092	arg1	Dendrogram					1095:1104	Dendrogram	1095:1104	Dendrogram	1095:1104	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	5	10	dep	analysis	1085:1092	arg1	maps					1112:1115	Heat maps	1107:1115	Heat maps	1107:1115	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	6	11	theme	cancerous	1304:1312	arg1	lines					1319:1323	PC3 cancerous cell lines	1300:1323	PC3 cancerous cell lines	1300:1323	MTT assay was employed to visualize the antiproliferative property against MCF7, HepG2 and PC3 cancerous cell lines.
37355085	3	12	theme	STUDY	587:591	arg1	AIM					576:578	AIM	576:578	AIM OF THE STUDY The current research	576:612	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	4	13	theme	Gas	925:927	arg1	system					944:949	a Clarus 580 Gas Chromatography system	912:949	a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS)	912:995	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	16	14	theme	anti-inflammatory	3367:3383	arg1	properties					3385:3394	antiproliferative and anti-inflammatory properties	3345:3394	antiproliferative and anti-inflammatory properties	3345:3394	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	6	15	theme	MTT	1209:1211	arg1	assay					1213:1217	MTT assay	1209:1217	MTT assay	1209:1217	MTT assay was employed to visualize the antiproliferative property against MCF7, HepG2 and PC3 cancerous cell lines.
37355085	10	16	theme	humulene	1907:1914	arg1	constituents					1983:1994	the major constituents	1973:1994	the major constituents	1973:1994	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	16	theme	humulene	1907:1914	arg1	%					1933:1933	19.86%	1928:1933	19.86%	1928:1933	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	16	theme	humulene	1907:1914	arg1	α-pinene					1888:1895	α-pinene	1888:1895	α-pinene (19.62%)	1888:1904	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	16	theme	humulene	1907:1914	arg1	1,8-cineole					1847:1857	1,8-cineole	1847:1857	1,8-cineole (52.71%)	1847:1866	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	16	theme	humulene	1907:1914	arg1	epoxide					1916:1922	humulene epoxide II	1907:1925	humulene epoxide II (19.86%)	1907:1934	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	16	theme	humulene	1907:1914	arg1	β-pinene					1869:1876	β-pinene	1869:1876	β-pinene (32.83%)	1869:1885	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	16	theme	humulene	1907:1914	arg1	epoxide					1949:1955	humulene epoxide I	1940:1957	humulene epoxide I (19.10%)	1940:1966	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	13	17	theme	MCF7	2328:2331	arg1	lines					2360:2364	MCF7, HepG2 and PC3 cancer cell lines	2328:2364	lines	2360:2364	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	13	18	theme	cell	2355:2358	arg1	lines					2360:2364	MCF7, HepG2 and PC3 cancer cell lines	2328:2364	lines	2360:2364	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	15	19	theme	anti-inflammatory	2857:2873	arg1	activity					2875:2882	remarkable anti-inflammatory activity	2846:2882	remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages	2846:2928	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	5	20	theme	data	1015:1018	arg1	statistics					1020:1029	The multivariate data statistics	998:1029	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means)	998:1125	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	10	21	theme	humulene	1940:1947	arg1	constituents					1983:1994	the major constituents	1973:1994	the major constituents	1973:1994	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	21	theme	humulene	1940:1947	arg1	α-pinene					1888:1895	α-pinene	1888:1895	α-pinene (19.62%)	1888:1904	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	21	theme	humulene	1940:1947	arg1	%					1965:1965	19.10%	1960:1965	19.10%	1960:1965	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	21	theme	humulene	1940:1947	arg1	1,8-cineole					1847:1857	1,8-cineole	1847:1857	1,8-cineole (52.71%)	1847:1866	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	21	theme	humulene	1940:1947	arg1	epoxide					1916:1922	humulene epoxide II	1907:1925	humulene epoxide II (19.86%)	1907:1934	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	21	theme	humulene	1940:1947	arg1	β-pinene					1869:1876	β-pinene	1869:1876	β-pinene (32.83%)	1869:1885	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	21	theme	humulene	1940:1947	arg1	epoxide					1949:1955	humulene epoxide I	1940:1957	humulene epoxide I (19.10%)	1940:1966	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	13	22	theme	human	2474:2478	arg1	breast					2480:2485	human breast and prostate adenocarcinoma cells	2474:2519	breast	2480:2485	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	10	23	theme	Hedychium	1826:1834	arg1	species					1815:1821	four targeted species	1801:1821	four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents	1801:1994	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	14	24	theme	3T3-L1	2630:2635	arg1	cells					2638:2642	normal (3T3-L1) cells	2622:2642	normal (3T3-L1) cells	2622:2642	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	15	25	theme	mediators	2975:2983	arg1	production					2948:2957	the production	2944:2957	the production of inflammatory mediators	2944:2983	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	3	26	from	species	769:775	arg1	oil					745:747	rhizome essential oil	727:747	rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	727:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	26	from	species	769:775	arg1	activities					713:722	the antiproliferative and anti-inflammatory activities	669:722	the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	669:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	0	27	theme	species	149:155	arg1	oil					127:129	rhizome essential oil	109:129	rhizome essential oil of four Hedychium species from India	109:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	1	28	theme	several	254:260	arg1	species					284:290	several perennial rhizomatous species	254:290	several perennial rhizomatous species widely used in perfumery and traditional folk medicine	254:345	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	16	29	theme	active	3323:3328	arg1	compounds					3330:3338	active compounds	3323:3338	active compounds	3323:3338	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	14	30	theme	normal	2622:2627	arg1	cells					2638:2642	normal (3T3-L1) cells	2622:2642	normal (3T3-L1) cells	2622:2642	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	4	31	theme	mass	971:974	arg1	spectrometry					976:987	mass spectrometry	971:987	mass spectrometry (GC-MS)	971:995	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	4	31	theme	mass	971:974	arg1	GC-MS					990:994	GC-MS	990:994	GC-MS	990:994	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	8	32	theme	essential	1535:1543	arg1	oils					1545:1548	Hedychium essential oils	1525:1548	Hedychium essential oils	1525:1548	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	13	33	dep	H.	2409:2410	arg1	gardnerianum					2412:2423	H. gardnerianum	2409:2423	H. gardnerianum	2409:2423	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	13	34	theme	highest	2435:2441	arg1	cytotoxicity					2453:2464	highest selective cytotoxicity	2435:2464	highest selective cytotoxicity against human breast and prostate adenocarcinoma cells	2435:2519	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	3	35	theme	chemical	635:642	arg1	composition					644:654	the chemical composition	631:654	the chemical composition	631:654	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	36	dep	STUDY	587:591	arg1	research					605:612	The current research	593:612	THE STUDY The current research	583:612	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	4	37	with	conjunction	954:964	arg1	spectrometry					976:987	mass spectrometry	971:987	mass spectrometry (GC-MS)	971:995	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	4	37	with	conjunction	954:964	arg1	GC-MS					990:994	GC-MS	990:994	GC-MS	990:994	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	0	38	from	oil	127:129	arg1	India					162:166	India	162:166	India	162:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	14	39	theme	side	2768:2771	arg1	effects					2773:2779	any side effects	2764:2779	any side effects	2764:2779	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	5	40	dep	chemometrics	1037:1048	arg1	sPLS-DA					1064:1070	sPLS-DA	1064:1070	sPLS-DA	1064:1070	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	5	40	dep	chemometrics	1037:1048	arg1	PLS-DA					1056:1061	PLS-DA	1056:1061	PLS-DA	1056:1061	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	5	40	dep	chemometrics	1037:1048	arg1	PCA					1051:1053	PCA	1051:1053	PCA	1051:1053	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	12	41	theme	multivariate	2160:2171	arg1	analysis					2173:2180	The multivariate analysis	2156:2180	The multivariate analysis	2156:2180	The multivariate analysis classified the four Hedychium species into three different clusters.
37355085	15	42	theme	essential	2795:2803	arg1	oils					2805:2808	the essential oils	2791:2808	the essential oils at 100 μg/mL concentration	2791:2835	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	5	43	theme	Heat	1107:1110	arg1	Dendrogram					1095:1104	Dendrogram	1095:1104	Dendrogram	1095:1104	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	5	43	theme	Heat	1107:1110	arg1	maps					1112:1115	Heat maps	1107:1115	Heat maps	1107:1115	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	13	44	theme	Hedychium	2251:2259	arg1	oils					2271:2274	Hedychium essential oils	2251:2274	Hedychium essential oils	2251:2274	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	2	45	theme	biological	553:562	arg1	activities					564:573	biological activities	553:573	biological activities	553:573	Several species of Hedychium have remained under-explored with respect to their chemical composition and biological activities.
37355085	12	46	theme	different	2231:2239	arg1	clusters					2241:2248	three different clusters	2225:2248	three different clusters	2225:2248	The multivariate analysis classified the four Hedychium species into three different clusters.
37355085	4	47	from	system	944:949	arg1	conjunction					954:964	conjunction	954:964	conjunction with mass spectrometry (GC-MS)	954:995	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	8	48	theme	oxide	1588:1592	arg1	production					1567:1576	the production	1563:1576	the production of nitric oxide (NO)	1563:1597	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	0	49	theme	rhizome	109:115	arg1	oil					127:129	rhizome essential oil	109:129	rhizome essential oil of four Hedychium species from India	109:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	3	50	dep	H.	824:825	arg1	griffithianum					827:839	H. griffithianum	824:839	H. griffithianum	824:839	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	16	51	with	source	3313:3318	arg1	properties					3385:3394	antiproliferative and anti-inflammatory properties	3345:3394	antiproliferative and anti-inflammatory properties	3345:3394	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	12	52	theme	Hedychium	2202:2210	arg1	species					2212:2218	the four Hedychium species	2193:2218	the four Hedychium species	2193:2218	The multivariate analysis classified the four Hedychium species into three different clusters.
37355085	13	53	theme	prostate	2491:2498	arg1	cells					2515:2519	human breast and prostate adenocarcinoma cells	2474:2519	cells	2515:2519	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	0	54	theme	oil	127:129	arg1	activities					95:104	antiproliferative and anti-inflammatory activities	55:104	antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India	55:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	0	54	theme	oil	127:129	arg1	analysis					45:52	multivariate analysis	32:52	multivariate analysis	32:52	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	1	55	theme	stomach	403:409	arg1	disorders					411:419	stomach disorders	403:419	stomach disorders	403:419	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	7	56	theme	non-tumorigenic/normal	1385:1406	arg1	cells					1408:1412	3T3-L1 non-tumorigenic/normal cells	1378:1412	3T3-L1 non-tumorigenic/normal cells	1378:1412	The toxicity of essential oils was determined using 3T3-L1 non-tumorigenic/normal cells.
37355085	0	57	theme	Volatile	0:7	arg1	profiling					9:17	Volatile profiling	0:17	Volatile profiling	0:17	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	1	58	theme	traditional	321:331	arg1	medicine					338:345	traditional folk medicine	321:345	traditional folk medicine	321:345	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	4	59	theme	Clarus	914:919	arg1	system					944:949	a Clarus 580 Gas Chromatography system	912:949	a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS)	912:995	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	15	60	theme	maximum	3017:3023	arg1	response					3043:3050	the maximum anti-inflammation response	3013:3050	the maximum anti-inflammation response	3013:3050	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	8	61	theme	Griess	1609:1614	arg1	method					1624:1629	the Griess reagent method	1605:1629	the Griess reagent method	1605:1629	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	8	62	theme	RAW	1448:1450	arg1	264.7 cells					1452:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells	1415:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells	1415:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	15	63	theme	IL-1β	3147:3151	arg1	levels					3085:3090	the levels	3081:3090	the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group	3081:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	11	64	theme	extracted	2130:2138	arg1	oils					2150:2153	the extracted essential oils	2126:2153	the extracted essential oils	2126:2153	Monoterpenes (8.5-59.9%) and sesquiterpenes (2.87-54.11%) were the two class of compounds that revealed as the most prevalent in the extracted essential oils.
37355085	3	65	theme	rhizome	727:733	arg1	oil					745:747	rhizome essential oil	727:747	rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	727:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	2	66	theme	Several	448:454	arg1	species					456:462	Several species	448:462	Several species of Hedychium	448:475	Several species of Hedychium have remained under-explored with respect to their chemical composition and biological activities.
37355085	0	67	theme	multivariate	32:43	arg1	activities					95:104	antiproliferative and anti-inflammatory activities	55:104	antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India	55:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	0	67	theme	multivariate	32:43	arg1	analysis					45:52	multivariate analysis	32:52	multivariate analysis	32:52	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	5	68	theme	Hedychium	1186:1194	arg1	metabolomes					1196:1206	Hedychium metabolomes	1186:1206	Hedychium metabolomes	1186:1206	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	9	69	theme	prostaglandin	1659:1671	arg1	levels					1649:1654	the levels	1645:1654	the levels of prostaglandin (PGE2) and pro-inflammatory cytokines	1645:1709	Furthermore, the levels of prostaglandin (PGE2) and pro-inflammatory cytokines was assessed using the ELISA technique.
37355085	7	70	theme	essential	1342:1350	arg1	oils					1352:1355	essential oils	1342:1355	essential oils	1342:1355	The toxicity of essential oils was determined using 3T3-L1 non-tumorigenic/normal cells.
37355085	8	71	used	used	1469:1472	arg2	264.7 cells					1452:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells	1415:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells	1415:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	3	72	dep	H.	792:793	arg1	gardnerianum					795:806	H. gardnerianum	792:806	H. gardnerianum	792:806	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	13	73	theme	adenocarcinoma	2500:2513	arg1	cells					2515:2519	human breast and prostate adenocarcinoma cells	2474:2519	cells	2515:2519	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	1	74	dep	comprises	244:252	arg1	Zingiberaceae					230:242	Zingiberaceae	230:242	Zingiberaceae	230:242	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	5	75	theme	cluster	1077:1083	arg1	analysis					1085:1092	cluster analysis	1077:1092	cluster analysis (Dendrogram, Heat maps, K-means)	1077:1125	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	1	76	theme	perennial	262:270	arg1	species					284:290	several perennial rhizomatous species	254:290	several perennial rhizomatous species widely used in perfumery and traditional folk medicine	254:345	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	15	77	theme	IL-6	3132:3135	arg1	levels					3085:3090	the levels	3081:3090	the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group	3081:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	3	78	dep	species	769:775	arg1	H.					778:779	H.	778:779	H.	778:779	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	78	dep	species	769:775	arg1	H.					792:793	H.	792:793	H.	792:793	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	78	dep	species	769:775	arg1	H.					809:810	H.	809:810	H.	809:810	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	78	dep	species	769:775	arg1	H.					824:825	H.	824:825	H.	824:825	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	3	78	dep	species	769:775	arg1	species					769:775	four Hedychium species	754:775	four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	754:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	14	79	theme	IC50	2678:2681	arg1	values					2683:2688	higher IC50 values	2671:2688	higher IC50 values	2671:2688	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	3	80	theme	oil	745:747	arg1	activities					713:722	the antiproliferative and anti-inflammatory activities	669:722	the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	669:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	4	81	theme	Compound	865:872	arg1	identification					874:887	MATERIALS AND METHODS Compound identification	843:887	MATERIALS AND METHODS Compound identification	843:887	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	13	82	theme	56.11 ± 1.44 μg/mL	2566:2583	arg1	value					2534:2538	an IC50 value	2526:2538	an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL	2526:2583	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	15	83	theme	LPS	3174:3176	arg1	group					3186:3190	LPS treated group	3174:3190	LPS treated group	3174:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	9	84	theme	cytokines	1701:1709	arg1	levels					1649:1654	the levels	1645:1654	the levels of prostaglandin (PGE2) and pro-inflammatory cytokines	1645:1709	Furthermore, the levels of prostaglandin (PGE2) and pro-inflammatory cytokines was assessed using the ELISA technique.
37355085	10	85	from	RESULTS	1751:1757	arg1	total					1762:1766	total	1762:1766	total	1762:1766	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	85	from	RESULTS	1751:1757	arg1	compounds					1772:1780	82 compounds	1769:1780	82 compounds	1769:1780	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	4	86	theme	Chromatography	929:942	arg1	system					944:949	a Clarus 580 Gas Chromatography system	912:949	a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS)	912:995	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	15	87	from	concentration	2823:2835	arg1	oils					2805:2808	the essential oils	2791:2808	the essential oils at 100 μg/mL concentration	2791:2835	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	0	88	from	India	162:166	arg1	species					149:155	four Hedychium species	134:155	four Hedychium species from India	134:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	0	88	from	India	162:166	arg1	oil					127:129	rhizome essential oil	109:129	rhizome essential oil of four Hedychium species from India	109:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	16	89	dep	CONCLUSION	3193:3202	arg1	showed					3225:3230	showed	3225:3230	showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties	3225:3394	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	6	90	theme	cell	1314:1317	arg1	lines					1319:1323	PC3 cancerous cell lines	1300:1323	PC3 cancerous cell lines	1300:1323	MTT assay was employed to visualize the antiproliferative property against MCF7, HepG2 and PC3 cancerous cell lines.
37355085	10	91	theme	targeted	1806:1813	arg1	species					1815:1821	four targeted species	1801:1821	four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents	1801:1994	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	13	92	theme	antiproliferative	2293:2309	arg1	activity					2311:2318	potent antiproliferative activity	2286:2318	potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines	2286:2364	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	1	93	theme	related	365:371	arg1	diseases					356:363	diseases	356:363	diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours	356:445	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	11	94	from	prevalent	2113:2121	arg1	oils					2150:2153	the extracted essential oils	2126:2153	the extracted essential oils	2126:2153	Monoterpenes (8.5-59.9%) and sesquiterpenes (2.87-54.11%) were the two class of compounds that revealed as the most prevalent in the extracted essential oils.
37355085	6	95	theme	PC3	1300:1302	arg1	lines					1319:1323	PC3 cancerous cell lines	1300:1323	PC3 cancerous cell lines	1300:1323	MTT assay was employed to visualize the antiproliferative property against MCF7, HepG2 and PC3 cancerous cell lines.
37355085	11	96	theme	compounds	2077:2085	arg1	sesquiterpenes					2026:2039	sesquiterpenes	2026:2039	sesquiterpenes (2.87-54.11%)	2026:2053	Monoterpenes (8.5-59.9%) and sesquiterpenes (2.87-54.11%) were the two class of compounds that revealed as the most prevalent in the extracted essential oils.
37355085	11	96	theme	compounds	2077:2085	arg1	Monoterpenes					1997:2008	Monoterpenes	1997:2008	Monoterpenes (8.5-59.9%)	1997:2020	Monoterpenes (8.5-59.9%) and sesquiterpenes (2.87-54.11%) were the two class of compounds that revealed as the most prevalent in the extracted essential oils.
37355085	11	96	theme	compounds	2077:2085	arg1	class					2068:2072	the two class	2060:2072	the two class of compounds that revealed as the most prevalent in the extracted essential oils	2060:2153	Monoterpenes (8.5-59.9%) and sesquiterpenes (2.87-54.11%) were the two class of compounds that revealed as the most prevalent in the extracted essential oils.
37355085	15	97	theme	LPS-activated	2887:2899	arg1	macrophages					2918:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	13	98	theme	IC50	2371:2374	arg1	values					2376:2381	IC50 values	2371:2381	IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively	2371:2597	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	0	99	theme	Hedychium	139:147	arg1	species					149:155	four Hedychium species	134:155	four Hedychium species from India	134:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	1	100	theme	family	223:228	arg1	RELEVANCE					190:198	ETHNOPHARMACOLOGICAL RELEVANCE	169:198	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family	169:228	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	15	101	theme	PGE2	3105:3108	arg1	levels					3085:3090	the levels	3081:3090	the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group	3081:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	8	102	theme	Hedychium	1525:1533	arg1	oils					1545:1548	Hedychium essential oils	1525:1548	Hedychium essential oils	1525:1548	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	15	103	dep	H.	2991:2992	arg1	greenii					2994:3000	H. greenii	2991:3000	H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group	2991:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	15	104	theme	100 μg/mL	2813:2821	arg1	concentration					2823:2835	100 μg/mL concentration	2813:2835	100 μg/mL concentration	2813:2835	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	8	105	theme	oils	1545:1548	arg1	properties					1511:1520	the anti-inflammatory properties	1489:1520	the anti-inflammatory properties of Hedychium essential oils	1489:1548	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	15	106	theme	inflammatory	2962:2973	arg1	mediators					2975:2983	inflammatory mediators	2962:2983	inflammatory mediators	2962:2983	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	13	107	theme	selective	2443:2451	arg1	cytotoxicity					2453:2464	highest selective cytotoxicity	2435:2464	highest selective cytotoxicity against human breast and prostate adenocarcinoma cells	2435:2519	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	3	108	theme	antiproliferative	673:689	arg1	activities					713:722	the antiproliferative and anti-inflammatory activities	669:722	the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	669:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	15	109	from	activity	2875:2882	arg1	macrophages					2918:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	LPS-activated RAW 264.7 murine macrophages	2887:2928	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	14	110	theme	essential	2604:2612	arg1	oils					2614:2617	The essential oils	2600:2617	The essential oils on normal (3T3-L1) cells	2600:2642	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	6	111	theme	antiproliferative	1249:1265	arg1	property					1267:1274	the antiproliferative property	1245:1274	the antiproliferative property against MCF7, HepG2 and PC3 cancerous cell lines	1245:1323	MTT assay was employed to visualize the antiproliferative property against MCF7, HepG2 and PC3 cancerous cell lines.
37355085	10	112	theme	major	1977:1981	arg1	constituents					1983:1994	the major constituents	1973:1994	the major constituents	1973:1994	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	112	theme	major	1977:1981	arg1	α-pinene					1888:1895	α-pinene	1888:1895	α-pinene (19.62%)	1888:1904	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	112	theme	major	1977:1981	arg1	1,8-cineole					1847:1857	1,8-cineole	1847:1857	1,8-cineole (52.71%)	1847:1866	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	112	theme	major	1977:1981	arg1	epoxide					1916:1922	humulene epoxide II	1907:1925	humulene epoxide II (19.86%)	1907:1934	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	112	theme	major	1977:1981	arg1	β-pinene					1869:1876	β-pinene	1869:1876	β-pinene (32.83%)	1869:1885	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	112	theme	major	1977:1981	arg1	epoxide					1949:1955	humulene epoxide I	1940:1957	humulene epoxide I (19.10%)	1940:1966	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	0	113	theme	anti-inflammatory	77:93	arg1	activities					95:104	antiproliferative and anti-inflammatory activities	55:104	antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India	55:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	0	113	theme	anti-inflammatory	77:93	arg1	analysis					45:52	multivariate analysis	32:52	multivariate analysis	32:52	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	13	114	theme	essential	2261:2269	arg1	oils					2271:2274	Hedychium essential oils	2251:2274	Hedychium essential oils	2251:2274	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	8	115	theme	nitric	1581:1586	arg1	oxide					1588:1592	nitric oxide	1581:1592	nitric oxide (NO)	1581:1597	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	8	115	theme	nitric	1581:1586	arg1	NO					1595:1596	NO	1595:1596	NO	1595:1596	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	5	116	used	used	1132:1135	arg2	statistics					1020:1029	The multivariate data statistics	998:1029	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means)	998:1125	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	3	117	dep	H.	809:810	arg1	greenii					812:818	H. greenii	809:818	H. greenii	809:818	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	1	118	theme	ETHNOPHARMACOLOGICAL	169:188	arg1	RELEVANCE					190:198	ETHNOPHARMACOLOGICAL RELEVANCE	169:198	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family	169:228	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	10	119	from	Hedychium	1826:1834	arg1	constituents					1983:1994	the major constituents	1973:1994	the major constituents	1973:1994	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	119	from	Hedychium	1826:1834	arg1	α-pinene					1888:1895	α-pinene	1888:1895	α-pinene (19.62%)	1888:1904	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	119	from	Hedychium	1826:1834	arg1	1,8-cineole					1847:1857	1,8-cineole	1847:1857	1,8-cineole (52.71%)	1847:1866	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	119	from	Hedychium	1826:1834	arg1	epoxide					1916:1922	humulene epoxide II	1907:1925	humulene epoxide II (19.86%)	1907:1934	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	119	from	Hedychium	1826:1834	arg1	β-pinene					1869:1876	β-pinene	1869:1876	β-pinene (32.83%)	1869:1885	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	10	119	from	Hedychium	1826:1834	arg1	epoxide					1949:1955	humulene epoxide I	1940:1957	humulene epoxide I (19.10%)	1940:1966	RESULTS In total, 82 compounds were identified in four targeted species of Hedychium from which 1,8-cineole (52.71%), β-pinene (32.83%), α-pinene (19.62%), humulene epoxide II (19.86%) and humulene epoxide I (19.10%) were the major constituents.
37355085	15	120	theme	TNF-α	3117:3121	arg1	levels					3085:3090	the levels	3081:3090	the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group	3081:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	2	121	theme	chemical	528:535	arg1	composition					537:547	their chemical composition	522:547	their chemical composition	522:547	Several species of Hedychium have remained under-explored with respect to their chemical composition and biological activities.
37355085	16	122	theme	Hedychium	3241:3249	arg1	species					3251:3257	the Hedychium species	3237:3257	the Hedychium species traditionally used in therapeutics	3237:3292	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	16	122	theme	Hedychium	3241:3249	arg1	source					3313:3318	potential source	3303:3318	potential source of active compounds with antiproliferative and anti-inflammatory properties	3303:3394	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	0	123	theme	essential	117:125	arg1	oil					127:129	rhizome essential oil	109:129	rhizome essential oil of four Hedychium species from India	109:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	13	124	theme	HepG2	2334:2338	arg1	lines					2360:2364	MCF7, HepG2 and PC3 cancer cell lines	2328:2364	lines	2360:2364	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	15	125	dep	levels	3085:3090	arg1	compared					3162:3169	compared	3162:3169	compared to LPS treated group	3162:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	15	126	theme	remarkable	2846:2855	arg1	activity					2875:2882	remarkable anti-inflammatory activity	2846:2882	remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages	2846:2928	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	7	127	theme	3T3-L1	1378:1383	arg1	cells					1408:1412	3T3-L1 non-tumorigenic/normal cells	1378:1412	3T3-L1 non-tumorigenic/normal cells	1378:1412	The toxicity of essential oils was determined using 3T3-L1 non-tumorigenic/normal cells.
37355085	5	128	theme	multivariate	1002:1013	arg1	statistics					1020:1029	The multivariate data statistics	998:1029	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means)	998:1125	The multivariate data statistics using chemometrics (PCA, PLS-DA, sPLS-DA) and cluster analysis (Dendrogram, Heat maps, K-means) were used to compare the similarity and relationship among Hedychium metabolomes.
37355085	16	129	theme	present	3208:3214	arg1	findings					3216:3223	The present findings	3204:3223	The present findings	3204:3223	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	14	130	theme	normal	2728:2733	arg1	health					2741:2746	normal human health	2728:2746	normal human health	2728:2746	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	8	131	theme	reagent	1616:1622	arg1	method					1624:1629	the Griess reagent method	1605:1629	the Griess reagent method	1605:1629	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	1	132	dep	RELEVANCE	190:198	arg1	Hedychium					210:218	The genus Hedychium	200:218	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family	169:228	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	1	133	theme	folk	333:336	arg1	medicine					338:345	traditional folk medicine	321:345	traditional folk medicine	321:345	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	13	134	theme	less	2383:2386	arg1	values					2376:2381	IC50 values	2371:2381	IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively	2371:2597	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	7	135	theme	oils	1352:1355	arg1	toxicity					1330:1337	The toxicity	1326:1337	The toxicity of essential oils	1326:1355	The toxicity of essential oils was determined using 3T3-L1 non-tumorigenic/normal cells.
37355085	2	136	theme	Hedychium	467:475	arg1	species					456:462	Several species	448:462	Several species of Hedychium	448:475	Several species of Hedychium have remained under-explored with respect to their chemical composition and biological activities.
37355085	8	137	theme	-induced	1439:1446	arg1	264.7 cells					1452:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells	1415:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells	1415:1462	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	13	138	theme	44.04 ± 1.07 μg/mL	2543:2560	arg1	value					2534:2538	an IC50 value	2526:2538	an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL	2526:2583	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	3	139	dep	H.	778:779	arg1	coccineum					781:789	H. coccineum	778:789	H. coccineum	778:789	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	13	140	theme	cancer	2348:2353	arg1	lines					2360:2364	MCF7, HepG2 and PC3 cancer cell lines	2328:2364	lines	2360:2364	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	11	141	theme	essential	2140:2148	arg1	oils					2150:2153	the extracted essential oils	2126:2153	the extracted essential oils	2126:2153	Monoterpenes (8.5-59.9%) and sesquiterpenes (2.87-54.11%) were the two class of compounds that revealed as the most prevalent in the extracted essential oils.
37355085	14	142	from	oils	2614:2617	arg1	cells					2638:2642	normal (3T3-L1) cells	2622:2642	normal (3T3-L1) cells	2622:2642	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	3	143	theme	anti-inflammatory	695:711	arg1	activities					713:722	the antiproliferative and anti-inflammatory activities	669:722	the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	669:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	0	144	theme	antiproliferative	55:71	arg1	activities					95:104	antiproliferative and anti-inflammatory activities	55:104	antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India	55:166	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	0	144	theme	antiproliferative	55:71	arg1	analysis					45:52	multivariate analysis	32:52	multivariate analysis	32:52	Volatile profiling coupled with multivariate analysis, antiproliferative and anti-inflammatory activities of rhizome essential oil of four Hedychium species from India.
37355085	13	145	theme	PC3	2344:2346	arg1	lines					2360:2364	MCF7, HepG2 and PC3 cancer cell lines	2328:2364	lines	2360:2364	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	15	146	theme	NO	3095:3096	arg1	levels					3085:3090	the levels	3081:3090	the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group	3081:3190	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
37355085	11	147	from	oils	2150:2153	arg1	prevalent					2113:2121	prevalent	2113:2121	prevalent	2113:2121	Monoterpenes (8.5-59.9%) and sesquiterpenes (2.87-54.11%) were the two class of compounds that revealed as the most prevalent in the extracted essential oils.
37355085	4	148	theme	METHODS	857:863	arg1	identification					874:887	MATERIALS AND METHODS Compound identification	843:887	MATERIALS AND METHODS Compound identification	843:887	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	16	149	theme	potential	3303:3311	arg1	source					3313:3318	potential source	3303:3318	potential source of active compounds with antiproliferative and anti-inflammatory properties	3303:3394	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	16	149	theme	potential	3303:3311	arg1	species					3251:3257	the Hedychium species	3237:3257	the Hedychium species traditionally used in therapeutics	3237:3292	CONCLUSION The present findings showed that the Hedychium species traditionally used in therapeutics could be potential source of active compounds with antiproliferative and anti-inflammatory properties.
37355085	3	150	theme	essential	735:743	arg1	oil					745:747	rhizome essential oil	727:747	rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum)	727:840	AIM OF THE STUDY The current research aimed to explore the chemical composition and evaluate the antiproliferative and anti-inflammatory activities of rhizome essential oil from four Hedychium species (H. coccineum, H. gardnerianum, H. greenii and H. griffithianum).
37355085	4	151	theme	MATERIALS	843:851	arg1	identification					874:887	MATERIALS AND METHODS Compound identification	843:887	MATERIALS AND METHODS Compound identification	843:887	MATERIALS AND METHODS Compound identification was accomplished using a Clarus 580 Gas Chromatography system in conjunction with mass spectrometry (GC-MS).
37355085	1	152	theme	rhizomatous	272:282	arg1	species					284:290	several perennial rhizomatous species	254:290	several perennial rhizomatous species widely used in perfumery and traditional folk medicine	254:345	ETHNOPHARMACOLOGICAL RELEVANCE The genus Hedychium of family Zingiberaceae comprises several perennial rhizomatous species widely used in perfumery and traditional folk medicine to treat diseases related to asthma, diarrhoea, nausea, stomach disorders, inflammation and tumours.
37355085	13	153	theme	potent	2286:2291	arg1	activity					2311:2318	potent antiproliferative activity	2286:2318	potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines	2286:2364	Hedychium essential oils exhibited potent antiproliferative activity against MCF7, HepG2 and PC3 cancer cell lines with IC50 values less than 150 μg/mL where H. gardnerianum exhibited highest selective cytotoxicity against human breast and prostate adenocarcinoma cells with an IC50 value of 44.04 ± 1.07 μg/mL and 56.11 ± 1.44 μg/mL, respectively.
37355085	9	154	theme	pro-inflammatory	1684:1699	arg1	cytokines					1701:1709	pro-inflammatory cytokines	1684:1709	pro-inflammatory cytokines	1684:1709	Furthermore, the levels of prostaglandin (PGE2) and pro-inflammatory cytokines was assessed using the ELISA technique.
37355085	14	155	theme	higher	2671:2676	arg1	values					2683:2688	higher IC50 values	2671:2688	higher IC50 values	2671:2688	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	14	156	theme	human	2735:2739	arg1	health					2741:2746	normal human health	2728:2746	normal human health	2728:2746	The essential oils on normal (3T3-L1) cells displayed no toxicity with higher IC50 values thereby concluding as safe to use for normal human health without causing any side effects.
37355085	8	157	theme	anti-inflammatory	1493:1509	arg1	properties					1511:1520	the anti-inflammatory properties	1489:1520	the anti-inflammatory properties of Hedychium essential oils	1489:1548	Lipopolysaccharide (LPS)-induced RAW 264.7 cells were used to investigate the anti-inflammatory properties of Hedychium essential oils by measuring the production of nitric oxide (NO) using the Griess reagent method.
37355085	15	158	theme	anti-inflammation	3025:3041	arg1	response					3043:3050	the maximum anti-inflammation response	3013:3050	the maximum anti-inflammation response	3013:3050	Besides, the essential oils at 100 μg/mL concentration revealed remarkable anti-inflammatory activity in LPS-activated RAW 264.7 murine macrophages by inhibiting the production of inflammatory mediators, with H. greenii exhibiting the maximum anti-inflammation response by significantly suppressing the levels of NO (84%), PGE2 (87%), TNF-α (94.3%), IL-6 (95%) and IL-1β (85%) as compared to LPS treated group.
35836199	12	0	theme	milk	1642:1645	arg1	RESULTS					1611:1617	RESULTS The MIR and ultrasonic milk analyzer results	1611:1662	RESULTS The MIR and ultrasonic milk analyzer results	1611:1662	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	12	0	theme	milk	1642:1645	arg1	analyzer					1647:1654	ultrasonic milk analyzer	1631:1654	ultrasonic milk analyzer	1631:1654	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	14	1	theme	measured	1913:1920	arg1	MIR					1922:1924	the measured MIR	1909:1924	the measured MIR	1909:1924	The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA.
35836199	11	2	theme	method	1397:1402	arg1	results					1371:1377	The results	1367:1377	The results of the ultrasonic method	1367:1402	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	2	3	theme	early	336:340	arg1	infancy					342:348	early infancy	336:348	early infancy	336:348	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	9	4	theme	milk	1273:1276	arg1	composition					1292:1302	the human milk macronutrient composition	1263:1302	the human milk macronutrient composition	1263:1302	HMA 3000, Hebei, CHINA) were used to determine the human milk macronutrient composition.
35836199	2	5	from	source	313:318	arg1	infancy					342:348	early infancy	336:348	early infancy	336:348	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	4	6	theme	milk	663:666	arg1	energy					668:673	milk energy	663:673	milk energy	663:673	Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance.
35836199	14	7	theme	scatter	2015:2021	arg1	diagram					2023:2029	the scatter diagram	2011:2029	the scatter diagram	2011:2029	The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA.
35836199	18	8	theme	breast	2600:2605	arg1	analyzers					2618:2626	the breast milk rapid analyzers	2596:2626	the breast milk rapid analyzers using the two methods	2596:2648	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	8	9	theme	MIR	1115:1117	arg1	analyzer					1124:1131	A MIR milk analyzer	1113:1131	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA)	1113:1167	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	0	10	theme	data	154:157	arg1	fitting					159:165	data fitting	154:165	data fitting	154:165	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	3	11	theme	nutritional	529:539	arg1	fulfillment					541:551	its nutritional fulfillment to preterm infant needs	525:575	its nutritional fulfillment to preterm infant needs	525:575	Some macronutrients composition in human breast milk varies greatly, which could affect its nutritional fulfillment to preterm infant needs.
35836199	3	12	dep	infant	564:569	arg1	needs					571:575	needs	571:575	needs	571:575	Some macronutrients composition in human breast milk varies greatly, which could affect its nutritional fulfillment to preterm infant needs.
35836199	16	13	theme	95	2278:2279	arg1	%					2280:2280	%	2280:2280	%	2280:2280	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	13	14	theme	ultrasonic	1796:1805	arg1	method					1807:1812	the ultrasonic method	1792:1812	the ultrasonic method	1792:1812	The protein, fat, and energy determined using the MIR method were higher than those determined by the ultrasonic method, while lactose determined by the MIR method were lower (all p < 0.05).
35836199	14	15	theme	Bland-Altman	1985:1996	arg1	analysis					1998:2005	the Bland-Altman analysis	1981:2005	the Bland-Altman analysis	1981:2005	The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA.
35836199	11	16	dep	method	1587:1592	arg1	results					1602:1608	results	1602:1608	results	1602:1608	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	3	17	theme	human	472:476	arg1	milk					485:488	human breast milk	472:488	human breast milk	472:488	Some macronutrients composition in human breast milk varies greatly, which could affect its nutritional fulfillment to preterm infant needs.
35836199	4	18	from	analysis	595:602	arg1	milk					685:688	breast milk	678:688	breast milk	678:688	Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance.
35836199	8	19	theme	BETTERREN	1134:1142	arg1	SH					1158:1159	SH	1158:1159	SH	1158:1159	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	8	19	theme	BETTERREN	1134:1142	arg1	HMIR-05					1149:1155	HMIR-05	1149:1155	HMIR-05	1149:1155	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	8	19	theme	BETTERREN	1134:1142	arg1	Co.					1144:1146	BETTERREN Co.	1134:1146	BETTERREN Co.	1134:1146	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	8	19	theme	BETTERREN	1134:1142	arg1	CHINA					1162:1166	CHINA	1162:1166	CHINA	1162:1166	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	6	20	theme	cross-sectional	900:914	arg1	study					916:920	This cross-sectional study	895:920	METHODS This cross-sectional study	887:920	METHODS This cross-sectional study included breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021.
35836199	4	21	theme	macronutrients	607:620	arg1	analysis					595:602	rapid analysis	589:602	rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk	589:688	Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance.
35836199	0	22	theme	learning	142:149	arg1	analyzers					51:59	analyzers	51:59	analyzers based on mid-infrared spectroscopy and ultrasound	51:109	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	0	22	theme	learning	142:149	arg1	application					119:129	the application	115:129	the application of machine learning in data fitting	115:165	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	17	23	with	consistent	2488:2497	arg1	results					2512:2518	the MIR results	2504:2518	the MIR results	2504:2518	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	5	24	theme	analyzer	788:795	arg1	results					756:762	the macronutrients results	737:762	the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer	737:840	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	16	25	theme	protein	2289:2295	arg1	LOA					2282:2284	The 95% LOA	2274:2284	The 95% LOA of protein, fat, lactose and energy	2274:2320	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	16	25	theme	protein	2289:2295	arg1	0.6 g/dl					2336:2343	0.6 g/dl	2336:2343	0.6 g/dl	2336:2343	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	6	26	dep	METHODS	887:893	arg1	study					916:920	This cross-sectional study	895:920	METHODS This cross-sectional study	887:920	METHODS This cross-sectional study included breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021.
35836199	8	27	theme	milk	1187:1190	arg1	analyzer					1192:1199	an ultrasonic milk analyzer	1173:1199	an ultrasonic milk analyzer (Honɡyanɡ Co,	1173:1213	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	7	28	theme	Breast	1014:1019	arg1	samples					1026:1032	Breast milk samples	1014:1032	Breast milk samples (n = 546)	1014:1042	Breast milk samples (n = 546) were collected from 244 mothers (from Day 1 to Day 1086 postpartum).
35836199	7	28	theme	Breast	1014:1019	arg1	n = 546					1035:1041	n = 546	1035:1041	n = 546	1035:1041	Breast milk samples (n = 546) were collected from 244 mothers (from Day 1 to Day 1086 postpartum).
35836199	15	29	theme	%	2135:2135	arg1	points					2141:2146	94.41% fat points	2130:2146	94.41% fat points (439 out of 465)	2130:2163	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	16	30	dep	0.6 g/dl	2336:2343	arg1	to					2333:2334	to	2333:2334	to	2333:2334	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	16	31	theme	fat	2298:2300	arg1	LOA					2282:2284	The 95% LOA	2274:2284	The 95% LOA of protein, fat, lactose and energy	2274:2320	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	16	31	theme	fat	2298:2300	arg1	0.6 g/dl					2336:2343	0.6 g/dl	2336:2343	0.6 g/dl	2336:2343	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	19	32	theme	Machine	2792:2798	arg1	methods					2809:2815	Machine learning methods	2792:2815	Machine learning methods	2792:2815	Machine learning methods can play a role in data fitting using different analyzers.
35836199	11	33	theme	MIR	1598:1600	arg1	results					1561:1567	the adjusted results	1548:1567	the adjusted results of the ultrasonic method and MIR results	1548:1608	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	1	34	from	carbohydrates	183:195	arg1	milk					236:239	breast milk	229:239	breast milk	229:239	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	5	35	theme	machine	869:875	arg1	learning					877:884	machine learning	869:884	machine learning	869:884	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	10	36	from	tests	1342:1346	arg1	analyzers					1356:1364	both analyzers	1351:1364	both analyzers	1351:1364	A total of 465 samples completed the tests in both analyzers.
35836199	11	37	theme	method	1587:1592	arg1	results					1561:1567	the adjusted results	1548:1567	the adjusted results of the ultrasonic method and MIR results	1548:1608	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	9	38	theme	macronutrient	1278:1290	arg1	composition					1292:1302	the human milk macronutrient composition	1263:1302	the human milk macronutrient composition	1263:1302	HMA 3000, Hebei, CHINA) were used to determine the human milk macronutrient composition.
35836199	15	39	theme	lactose	2173:2179	arg1	points					2181:2186	95.91% lactose points	2166:2186	95.91% lactose points (446 out of 465)	2166:2203	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	5	40	theme	mid-infrared	769:780	arg1	analyzer					788:795	a mid-infrared (MIR) analyzer	767:795	a mid-infrared (MIR) analyzer	767:795	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	5	40	theme	mid-infrared	769:780	arg1	MIR					783:785	MIR	783:785	MIR	783:785	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	1	41	from	Fat	178:180	arg1	milk					236:239	breast milk	229:239	breast milk	229:239	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	5	42	theme	analyzer	833:840	arg1	results					756:762	the macronutrients results	737:762	the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer	737:840	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	15	43	theme	95.91	2166:2170	arg1	%					2171:2171	%	2171:2171	%	2171:2171	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	1	44	theme	OBJECTIVE	168:176	arg1	Fat					178:180	OBJECTIVE Fat	168:180	OBJECTIVE Fat	168:180	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	17	45	theme	ultrasonic	2464:2473	arg1	results					2475:2481	The adjusted ultrasonic results	2451:2481	The adjusted ultrasonic results	2451:2481	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	17	45	theme	ultrasonic	2464:2473	arg1	consistent					2488:2497	consistent	2488:2497	consistent	2488:2497	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	14	46	theme	adjusted	1934:1941	arg1	values					1954:1959	the adjusted ultrasound values	1930:1959	the adjusted ultrasound values	1930:1959	The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA.
35836199	13	47	theme	MIR	1744:1746	arg1	method					1748:1753	the MIR method	1740:1753	the MIR method	1740:1753	The protein, fat, and energy determined using the MIR method were higher than those determined by the ultrasonic method, while lactose determined by the MIR method were lower (all p < 0.05).
35836199	0	48	from	analyzers	51:59	arg1	fitting					159:165	data fitting	154:165	data fitting	154:165	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	18	49	theme	analyzers	2618:2626	arg1	results					2585:2591	the results	2581:2591	the results of the breast milk rapid analyzers using the two methods	2581:2648	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	10	50	theme	samples	1320:1326	arg1	total					1307:1311	A total	1305:1311	A total of 465 samples	1305:1326	A total of 465 samples completed the tests in both analyzers.
35836199	15	51	theme	%	2214:2214	arg1	points					2223:2228	94.62% energy points	2209:2228	94.62% energy points (440 out of 465)	2209:2245	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	2	52	theme	milk	390:393	arg1	crucial					403:409	crucial	403:409	crucial	403:409	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	2	52	theme	milk	390:393	arg1	energy					373:378	the energy	369:378	the energy of breast milk	369:393	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	0	53	theme	milk	16:19	arg1	macronutrients					21:34	human milk macronutrients	10:34	human milk macronutrients	10:34	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	18	54	theme	rapid	2612:2616	arg1	analyzers					2618:2626	the breast milk rapid analyzers	2596:2626	the breast milk rapid analyzers using the two methods	2596:2648	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	16	55	dep	40.4	2399:2402	arg1	to					2396:2397	to	2396:2397	to	2396:2397	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	2	56	theme	infants	428:434	arg1	growth					418:423	the growth	414:423	the growth of infants	414:434	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	11	57	theme	adjusted	1552:1559	arg1	results					1561:1567	the adjusted results	1548:1567	the adjusted results of the ultrasonic method and MIR results	1548:1608	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	1	58	dep	carbohydrates	183:195	arg1	lactose					205:211	lactose	205:211	mainly lactose	198:211	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	14	59	theme	%	2064:2064	arg1	LOA					2066:2068	the 95% LOA	2058:2068	the 95% LOA	2058:2068	The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA.
35836199	7	60	theme	Day	1091:1093	arg1	postpartum					1100:1109	Day 1086 postpartum	1091:1109	Day 1086 postpartum	1091:1109	Breast milk samples (n = 546) were collected from 244 mothers (from Day 1 to Day 1086 postpartum).
35836199	1	61	theme	newborns	294:301	arg1	growth					284:289	the growth	280:289	the growth of newborns	280:301	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	8	62	dep	analyzer	1124:1131	arg1	SH					1158:1159	SH	1158:1159	SH	1158:1159	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	8	62	dep	analyzer	1124:1131	arg1	HMIR-05					1149:1155	HMIR-05	1149:1155	HMIR-05	1149:1155	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	8	62	dep	analyzer	1124:1131	arg1	Co.					1144:1146	BETTERREN Co.	1134:1146	BETTERREN Co.	1134:1146	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	8	62	dep	analyzer	1124:1131	arg1	CHINA					1162:1166	CHINA	1162:1166	CHINA	1162:1166	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	3	63	theme	macronutrients	442:455	arg1	composition					457:467	Some macronutrients composition	437:467	Some macronutrients composition in human breast milk	437:488	Some macronutrients composition in human breast milk varies greatly, which could affect its nutritional fulfillment to preterm infant needs.
35836199	5	64	theme	macronutrients	741:754	arg1	results					756:762	the macronutrients results	737:762	the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer	737:840	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	6	65	dep	aged	953:956	arg1	22-40					958:962	22-40	958:962	22-40	958:962	METHODS This cross-sectional study included breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021.
35836199	17	66	dep	high	2542:2545	arg1	%					2559:2559	close to 95%	2548:2559	close to 95%	2548:2559	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	16	67	theme	lactose	2303:2309	arg1	LOA					2282:2284	The 95% LOA	2274:2284	The 95% LOA of protein, fat, lactose and energy	2274:2320	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	16	67	theme	lactose	2303:2309	arg1	0.6 g/dl					2336:2343	0.6 g/dl	2336:2343	0.6 g/dl	2336:2343	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	11	68	theme	ultrasonic	1576:1585	arg1	method					1587:1592	the ultrasonic method	1572:1592	the ultrasonic method	1572:1592	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	17	69	theme	MIR	2508:2510	arg1	results					2512:2518	the MIR results	2504:2518	the MIR results	2504:2518	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	8	70	dep	analyzer	1192:1199	arg1	Co					1211:1212	Co	1211:1212	Co	1211:1212	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	12	71	theme	ultrasonic	1631:1640	arg1	RESULTS					1611:1617	RESULTS The MIR and ultrasonic milk analyzer results	1611:1662	RESULTS The MIR and ultrasonic milk analyzer results	1611:1662	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	12	71	theme	ultrasonic	1631:1640	arg1	analyzer					1647:1654	ultrasonic milk analyzer	1631:1654	ultrasonic milk analyzer	1631:1654	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	16	72	theme	energy	2315:2320	arg1	LOA					2282:2284	The 95% LOA	2274:2284	The 95% LOA of protein, fat, lactose and energy	2274:2320	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	16	72	theme	energy	2315:2320	arg1	0.6 g/dl					2336:2343	0.6 g/dl	2336:2343	0.6 g/dl	2336:2343	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	14	73	theme	95	2062:2063	arg1	%					2064:2064	%	2064:2064	%	2064:2064	The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA.
35836199	3	74	theme	breast	478:483	arg1	milk					485:488	human breast milk	472:488	human breast milk	472:488	Some macronutrients composition in human breast milk varies greatly, which could affect its nutritional fulfillment to preterm infant needs.
35836199	1	75	theme	breast	229:234	arg1	milk					236:239	breast milk	229:239	breast milk	229:239	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	17	76	dep	95	2557:2558	arg1	to					2554:2555	to	2554:2555	to	2554:2555	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	16	77	theme	%	2280:2280	arg1	LOA					2282:2284	The 95% LOA	2274:2284	The 95% LOA of protein, fat, lactose and energy	2274:2320	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	16	77	theme	%	2280:2280	arg1	0.6 g/dl					2336:2343	0.6 g/dl	2336:2343	0.6 g/dl	2336:2343	The 95% LOA of protein, fat, lactose and energy were - 0.6 to 0.6 g/dl, -0.92 to 0.92 g/dl, -0.88 to 0.88 g/dl and - 40.2 to 40.4 kj/dl, respectively and clinically acceptable.
35836199	17	78	theme	LOA	2525:2527	arg1	results					2529:2535	LOA results	2525:2535	LOA results	2525:2535	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	9	79	theme	human	1267:1271	arg1	composition					1292:1302	the human milk macronutrient composition	1263:1302	the human milk macronutrient composition	1263:1302	HMA 3000, Hebei, CHINA) were used to determine the human milk macronutrient composition.
35836199	19	80	theme	learning	2800:2807	arg1	methods					2809:2815	Machine learning methods	2792:2815	Machine learning methods	2792:2815	Machine learning methods can play a role in data fitting using different analyzers.
35836199	1	81	theme	indispensable	253:265	arg1	benefits					267:274	indispensable benefits	253:274	indispensable benefits for the growth of newborns	253:301	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	11	82	theme	ultrasonic	1386:1395	arg1	method					1397:1402	the ultrasonic method	1382:1402	the ultrasonic method	1382:1402	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	18	83	theme	data	2720:2723	arg1	adjustments					2725:2735	data adjustments	2720:2735	data adjustments using linear regression algorithm in machine learning	2720:2789	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	9	84	used	used	1245:1248	arg2	HMA					1216:1218	HMA	1216:1218	HMA	1216:1218	HMA 3000, Hebei, CHINA) were used to determine the human milk macronutrient composition.
35836199	9	84	used	used	1245:1248	arg2	CHINA					1233:1237	CHINA	1233:1237	CHINA	1233:1237	HMA 3000, Hebei, CHINA) were used to determine the human milk macronutrient composition.
35836199	9	84	used	used	1245:1248	arg2	Hebei					1226:1230	Hebei	1226:1230	Hebei	1226:1230	HMA 3000, Hebei, CHINA) were used to determine the human milk macronutrient composition.
35836199	11	85	theme	agreement	1524:1532	arg1	limits					1514:1519	the limits	1510:1519	the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results	1510:1608	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	11	86	theme	machine	1440:1446	arg1	learning					1448:1455	machine learning	1440:1455	machine learning	1440:1455	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	2	87	theme	only	308:311	arg1	source					313:318	The only source	304:318	The only source of nutrition in early infancy	304:348	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	2	87	theme	only	308:311	arg1	milk					360:363	breast milk	353:363	breast milk	353:363	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	5	88	theme	ultrasound-based	804:819	arg1	analyzer					833:840	an ultrasound-based breast milk analyzer	801:840	an ultrasound-based breast milk analyzer	801:840	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	6	89	theme	breastfeeding	931:943	arg1	mothers					945:951	breastfeeding mothers	931:951	breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021	931:1011	METHODS This cross-sectional study included breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021.
35836199	0	90	theme	machine	134:140	arg1	learning					142:149	machine learning	134:149	machine learning	134:149	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	3	91	from	composition	457:467	arg1	milk					485:488	human breast milk	472:488	human breast milk	472:488	Some macronutrients composition in human breast milk varies greatly, which could affect its nutritional fulfillment to preterm infant needs.
35836199	8	92	theme	milk	1119:1122	arg1	analyzer					1124:1131	A MIR milk analyzer	1113:1131	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA)	1113:1167	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	2	93	theme	breast	353:358	arg1	milk					360:363	breast milk	353:363	breast milk	353:363	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	2	93	theme	breast	353:358	arg1	source					313:318	The only source	304:318	The only source of nutrition in early infancy	304:348	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	4	94	theme	rapid	589:593	arg1	analysis					595:602	rapid analysis	589:602	rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk	589:688	Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance.
35836199	7	95	theme	milk	1021:1024	arg1	samples					1026:1032	Breast milk samples	1014:1032	Breast milk samples (n = 546)	1014:1042	Breast milk samples (n = 546) were collected from 244 mothers (from Day 1 to Day 1086 postpartum).
35836199	7	95	theme	milk	1021:1024	arg1	n = 546					1035:1041	n = 546	1035:1041	n = 546	1035:1041	Breast milk samples (n = 546) were collected from 244 mothers (from Day 1 to Day 1086 postpartum).
35836199	15	96	dep	points	2223:2228	arg1	465					2242:2244	465	2242:2244	465	2242:2244	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	5	97	theme	breast	821:826	arg1	analyzer					833:840	an ultrasound-based breast milk analyzer	801:840	an ultrasound-based breast milk analyzer	801:840	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	18	98	theme	regression	2750:2759	arg1	algorithm					2761:2769	linear regression algorithm	2743:2769	linear regression algorithm	2743:2769	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	15	99	theme	94.62	2209:2213	arg1	%					2214:2214	%	2214:2214	%	2214:2214	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	0	100	from	application	119:129	arg1	fitting					159:165	data fitting	154:165	data fitting	154:165	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	8	101	theme	ultrasonic	1176:1185	arg1	analyzer					1192:1199	an ultrasonic milk analyzer	1173:1199	an ultrasonic milk analyzer (Honɡyanɡ Co,	1173:1213	A MIR milk analyzer (BETTERREN Co., HMIR-05, SH, CHINA) and an ultrasonic milk analyzer (Honɡyanɡ Co,.
35836199	1	102	from	protein	218:224	arg1	milk					236:239	breast milk	229:239	breast milk	229:239	OBJECTIVE Fat, carbohydrates (mainly lactose) and protein in breast milk all provide indispensable benefits for the growth of newborns.
35836199	15	103	theme	fat	2137:2139	arg1	points					2141:2146	94.41% fat points	2130:2146	94.41% fat points (439 out of 465)	2130:2163	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	15	104	dep	points	2141:2146	arg1	465					2160:2162	465	2160:2162	465	2160:2162	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	4	105	theme	energy	668:673	arg1	analysis					595:602	rapid analysis	589:602	rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk	589:688	Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance.
35836199	0	106	theme	mid-infrared	70:81	arg1	spectroscopy					83:94	mid-infrared spectroscopy	70:94	mid-infrared spectroscopy	70:94	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	15	107	theme	94.41	2130:2134	arg1	%					2135:2135	%	2135:2135	%	2135:2135	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	5	108	theme	milk	828:831	arg1	analyzer					833:840	an ultrasound-based breast milk analyzer	801:840	an ultrasound-based breast milk analyzer	801:840	This study compared the macronutrients results of a mid-infrared (MIR) analyzer and an ultrasound-based breast milk analyzer and unified the results by machine learning.
35836199	7	109	from	Day	1082:1084	arg1	mothers					1068:1074	244 mothers	1064:1074	244 mothers (from Day 1 to Day 1086 postpartum)	1064:1110	Breast milk samples (n = 546) were collected from 244 mothers (from Day 1 to Day 1086 postpartum).
35836199	12	110	dep	MIR	1623:1625	arg1	The					1619:1621	The	1619:1621	The	1619:1621	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	12	110	dep	MIR	1623:1625	arg1	results					1656:1662	results	1656:1662	results	1656:1662	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	17	111	theme	adjusted	2455:2462	arg1	results					2475:2481	The adjusted ultrasonic results	2451:2481	The adjusted ultrasonic results	2451:2481	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	17	111	theme	adjusted	2455:2462	arg1	consistent					2488:2497	consistent	2488:2497	consistent	2488:2497	The adjusted ultrasonic results were consistent with the MIR results, and LOA results were high (close to 95%).
35836199	14	112	theme	ultrasound	1943:1952	arg1	values					1954:1959	the adjusted ultrasound values	1930:1959	the adjusted ultrasound values	1930:1959	The consistency between the measured MIR and the adjusted ultrasound values was evaluated using the Bland-Altman analysis and the scatter diagram was generated to calculate the 95% LOA.
35836199	12	113	dep	RESULTS	1611:1617	arg1	RESULTS					1611:1617	RESULTS The MIR and ultrasonic milk analyzer results	1611:1662	RESULTS The MIR and ultrasonic milk analyzer results	1611:1662	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	12	113	dep	RESULTS	1611:1617	arg1	analyzer					1647:1654	ultrasonic milk analyzer	1631:1654	ultrasonic milk analyzer	1631:1654	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	12	113	dep	RESULTS	1611:1617	arg1	MIR					1623:1625	MIR	1623:1625	MIR	1623:1625	RESULTS The MIR and ultrasonic milk analyzer results were significantly different.
35836199	15	114	theme	%	2171:2171	arg1	points					2181:2186	95.91% lactose points	2166:2186	95.91% lactose points (446 out of 465)	2166:2203	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	6	115	theme	aged	953:956	arg1	mothers					945:951	breastfeeding mothers	931:951	breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021	931:1011	METHODS This cross-sectional study included breastfeeding mothers aged 22-40 enrolled between November 2019 and February 2021.
35836199	4	116	theme	clinical	696:703	arg1	importance					705:714	clinical importance	696:714	clinical importance	696:714	Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance.
35836199	13	117	dep	lower	1863:1867	arg1	p < 0.05					1874:1881	p < 0.05	1874:1881	p < 0.05	1874:1881	The protein, fat, and energy determined using the MIR method were higher than those determined by the ultrasonic method, while lactose determined by the MIR method were lower (all p < 0.05).
35836199	0	118	theme	human	10:14	arg1	macronutrients					21:34	human milk macronutrients	10:34	human milk macronutrients	10:34	Comparing human milk macronutrients measured using analyzers based on mid-infrared spectroscopy and ultrasound and the application of machine learning in data fitting.
35836199	15	119	dep	points	2181:2186	arg1	465					2200:2202	465	2200:2202	465	2200:2202	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	10	120	theme	465	1316:1318	arg1	samples					1320:1326	465 samples	1316:1326	465 samples	1316:1326	A total of 465 samples completed the tests in both analyzers.
35836199	4	121	theme	breast	678:683	arg1	milk					685:688	breast milk	678:688	breast milk	678:688	Therefore, rapid analysis of macronutrients (including lactose, fat and protein) and milk energy in breast milk is of clinical importance.
35836199	19	122	theme	different	2855:2863	arg1	analyzers					2865:2873	different analyzers	2855:2873	different analyzers	2855:2873	Machine learning methods can play a role in data fitting using different analyzers.
35836199	15	123	theme	%	2095:2095	arg1	points					2105:2110	93.96% protein points	2090:2110	93.96% protein points (436 out of 465)	2090:2127	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	15	124	theme	energy	2216:2221	arg1	points					2223:2228	94.62% energy points	2209:2228	94.62% energy points (440 out of 465)	2209:2245	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	2	125	theme	breast	383:388	arg1	milk					390:393	breast milk	383:393	breast milk	383:393	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	18	126	theme	milk	2607:2610	arg1	analyzers					2618:2626	the breast milk rapid analyzers	2596:2626	the breast milk rapid analyzers using the two methods	2596:2648	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	11	127	used	used	1492:1495	arg2	method					1481:1486	the Bland-Altman method	1464:1486	the Bland-Altman method	1464:1486	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
35836199	18	128	theme	machine	2774:2780	arg1	learning					2782:2789	machine learning	2774:2789	machine learning	2774:2789	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	13	129	theme	MIR	1847:1849	arg1	method					1851:1856	the MIR method	1843:1856	the MIR method	1843:1856	The protein, fat, and energy determined using the MIR method were higher than those determined by the ultrasonic method, while lactose determined by the MIR method were lower (all p < 0.05).
35836199	2	130	theme	nutrition	323:331	arg1	source					313:318	The only source	304:318	The only source of nutrition in early infancy	304:348	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	2	130	theme	nutrition	323:331	arg1	milk					360:363	breast milk	353:363	breast milk	353:363	The only source of nutrition in early infancy is breast milk, so the energy of breast milk is also crucial to the growth of infants.
35836199	15	131	dep	points	2105:2110	arg1	465					2124:2126	465	2124:2126	465	2124:2126	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	15	132	theme	protein	2097:2103	arg1	points					2105:2110	93.96% protein points	2090:2110	93.96% protein points (436 out of 465)	2090:2127	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	15	133	theme	LOA	2263:2265	arg1	range					2267:2271	the LOA range	2259:2271	the LOA range	2259:2271	After adjustments, 93.96% protein points (436 out of 465), 94.41% fat points (439 out of 465), 95.91% lactose points (446 out of 465) and 94.62% energy points (440 out of 465) were within the LOA range.
35836199	18	134	theme	linear	2743:2748	arg1	algorithm					2761:2769	linear regression algorithm	2743:2769	linear regression algorithm	2743:2769	CONCLUSIONS While the results of the breast milk rapid analyzers using the two methods varied significantly, they could still be considered comparable after data adjustments using linear regression algorithm in machine learning.
35836199	11	135	theme	Bland-Altman	1468:1479	arg1	method					1481:1486	the Bland-Altman method	1464:1486	the Bland-Altman method	1464:1486	The results of the ultrasonic method were mathematically converted using machine learning, while the Bland-Altman method was used to determine the limits of agreement (LOA) between the adjusted results of the ultrasonic method and MIR results.
34986315	12	0	theme	neonatal	2011:2018	arg1	disease					2020:2026	neonatal disease	2011:2026	neonatal disease	2011:2026	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	7	1	theme	biofilm	1421:1427	arg1	formation					1429:1437	biofilm formation	1421:1437	biofilm formation	1421:1437	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	4	2	theme	treatment	811:819	arg1	impact					797:802	the impact	793:802	the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota	793:883	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	6	3	theme	inhibitory	1042:1051	arg1	activity					1053:1060	potent inhibitory activity	1035:1060	potent inhibitory activity	1035:1060	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	3	4	theme	milk	492:495	arg1	HMOs					515:518	HMOs	515:518	HMOs	515:518	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	3	4	theme	milk	492:495	arg1	oligosaccharides					497:512	human milk oligosaccharides	486:512	human milk oligosaccharides (HMOs) isolated from breastmilk	486:544	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	4	5	theme	GBS	824:826	arg1	burdens					828:834	GBS burdens	824:834	GBS burdens	824:834	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	0	6	with	Colonization	72:83	arg1	Impact					98:103	Minimal Impact	90:103	Minimal Impact on the Vaginal Microbiota	90:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	3	7	theme	human	486:490	arg1	HMOs					515:518	HMOs	515:518	HMOs	515:518	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	3	7	theme	human	486:490	arg1	oligosaccharides					497:512	human milk oligosaccharides	486:512	human milk oligosaccharides (HMOs) isolated from breastmilk	486:544	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	6	8	theme	vaginal	1186:1192	arg1	cells					1205:1209	human vaginal epithelial cells	1180:1209	human vaginal epithelial cells	1180:1209	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	14	9	theme	human	2334:2338	arg1	HMOs					2363:2366	HMOs	2363:2366	HMOs	2363:2366	Using a mouse model, we found that human milk oligosaccharides (HMOs) reduce GBS burdens without perturbing the vaginal microbiota.
34986315	14	9	theme	human	2334:2338	arg1	oligosaccharides					2345:2360	human milk oligosaccharides	2334:2360	human milk oligosaccharides (HMOs)	2334:2367	Using a mouse model, we found that human milk oligosaccharides (HMOs) reduce GBS burdens without perturbing the vaginal microbiota.
34986315	11	10	theme	broad-spectrum	1894:1907	arg1	antibiotics					1909:1919	broad-spectrum antibiotics	1894:1919	broad-spectrum antibiotics to GBS-positive mothers	1894:1943	Current recommended prophylaxis consists of administering broad-spectrum antibiotics to GBS-positive mothers during labor.
34986315	6	11	theme	HMO	1088:1090	arg1	pretreatment					1092:1103	HMO pretreatment	1088:1103	HMO pretreatment	1088:1103	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	12	12	theme	altered	2082:2088	arg1	bacteria					2103:2110	altered neonatal gut bacteria	2082:2110	altered neonatal gut bacteria	2082:2110	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	8	13	with	colonization	1544:1555	arg1	impacts					1570:1576	minimal impacts	1562:1576	minimal impacts on the vaginal microenvironment	1562:1608	We conclude that HMOs are a promising therapeutic bioactive to limit GBS vaginal colonization with minimal impacts on the vaginal microenvironment.
34986315	4	14	theme	microbiota	874:883	arg1	burdens					828:834	GBS burdens	824:834	GBS burdens	824:834	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	4	14	theme	microbiota	874:883	arg1	composition					844:854	the composition	840:854	the composition of the endogenous microbiota	840:883	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	6	15	theme	human	1180:1184	arg1	cells					1205:1209	human vaginal epithelial cells	1180:1209	human vaginal epithelial cells	1180:1209	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	12	16	theme	gut	2099:2101	arg1	bacteria					2103:2110	altered neonatal gut bacteria	2082:2110	altered neonatal gut bacteria	2082:2110	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	15	17	theme	promising	2459:2467	arg1	HMOs					2448:2451	HMOs	2448:2451	HMOs	2448:2451	We conclude that HMOs are a promising alternative to antibiotics to reduce GBS neonatal disease.
34986315	15	17	theme	promising	2459:2467	arg1	alternative					2469:2479	a promising alternative	2457:2479	a promising alternative to antibiotics to reduce GBS neonatal disease	2457:2525	We conclude that HMOs are a promising alternative to antibiotics to reduce GBS neonatal disease.
34986315	1	18	theme	percentage	206:215	arg1	mucosa					182:187	the vaginal mucosa	170:187	the vaginal mucosa of a significant percentage of healthy women	170:232	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	7	19	theme	resistant	1317:1325	arg1	bacterium					1299:1307	the bacterium	1295:1307	the bacterium largely resistant to HMO inhibition	1295:1343	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	12	20	theme	unintended	2047:2056	arg1	consequences					2058:2069	several unintended consequences	2039:2069	several unintended consequences	2039:2069	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	12	20	theme	unintended	2047:2056	arg1	bacteria					2103:2110	altered neonatal gut bacteria	2082:2110	altered neonatal gut bacteria	2082:2110	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	12	20	theme	unintended	2047:2056	arg1	risk					2126:2129	increased risk	2116:2129	increased risk for other types of infection	2116:2158	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	3	21	theme	GBS	568:570	arg1	growth					572:577	GBS growth	568:577	GBS growth	568:577	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	4	22	dep	in	702:703	arg1	vitro					705:709	vitro	705:709	vitro	705:709	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	1	23	theme	B	138:138	arg1	GBS					155:157	GBS	155:157	GBS	155:157	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	1	23	theme	B	138:138	arg1	Streptococcus					140:152	Group B Streptococcus	132:152	Group B Streptococcus (GBS)	132:158	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	9	24	theme	fetal	1680:1684	arg1	infection					1686:1694	fetal infection	1680:1694	fetal infection	1680:1694	IMPORTANCE During pregnancy, GBS ascension into the uterus can cause fetal infection or preterm birth.
34986315	0	25	from	Impact	98:103	arg1	Microbiota					120:129	the Vaginal Microbiota	108:129	the Vaginal Microbiota	108:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	0	26	theme	Vaginal	64:70	arg1	Colonization					72:83	Murine Group B Streptococcus Vaginal Colonization	35:83	Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota	35:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	13	27	theme	maternal	2251:2258	arg1	microbiota					2260:2269	the maternal microbiota	2247:2269	the maternal microbiota	2247:2269	Innovative preventions displaying more targeted antimicrobial activity, while leaving the maternal microbiota intact, are thus appealing.
34986315	4	28	theme	vaginal	717:723	arg1	cells					736:740	in vitro human vaginal epithelial cells	702:740	in vitro human vaginal epithelial cells	702:740	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	15	29	theme	GBS	2506:2508	arg1	disease					2519:2525	GBS neonatal disease	2506:2525	GBS neonatal disease	2506:2525	We conclude that HMOs are a promising alternative to antibiotics to reduce GBS neonatal disease.
34986315	4	30	theme	amplicon	897:904	arg1	sequencing					906:915	rRNA amplicon sequencing	892:915	rRNA amplicon sequencing	892:915	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	2	31	theme	GBS-positive	366:377	arg1	women					379:383	GBS-positive women	366:383	GBS-positive women	366:383	Currently, pregnant women are screened in the last month of pregnancy, and GBS-positive women are given antibiotics during parturition to prevent bacterial transmission to the neonate.
34986315	8	32	theme	GBS	1532:1534	arg1	colonization					1544:1555	GBS vaginal colonization	1532:1555	GBS vaginal colonization with minimal impacts on the vaginal microenvironment	1532:1608	We conclude that HMOs are a promising therapeutic bioactive to limit GBS vaginal colonization with minimal impacts on the vaginal microenvironment.
34986315	0	33	theme	Human	0:4	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	11	34	theme	GBS-positive	1924:1935	arg1	mothers					1937:1943	GBS-positive mothers	1924:1943	GBS-positive mothers	1924:1943	Current recommended prophylaxis consists of administering broad-spectrum antibiotics to GBS-positive mothers during labor.
34986315	1	35	theme	bacterial	269:277	arg1	infections					279:288	neonatal bacterial infections	260:288	neonatal bacterial infections	260:288	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	10	36	from	risk	1763:1766	arg1	mother					1817:1822	mother	1817:1822	mother	1817:1822	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	10	36	from	risk	1763:1766	arg1	newborn					1805:1811	newborn	1805:1811	newborn	1805:1811	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	7	37	theme	GBS	1250:1252	arg1	Δsan_0913					1275:1283	Δsan_0913	1275:1283	Δsan_0913	1275:1283	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	7	37	theme	GBS	1250:1252	arg1	glycosyltransferase					1254:1272	a putative GBS glycosyltransferase	1239:1272	a putative GBS glycosyltransferase (Δsan_0913)	1239:1284	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	10	38	theme	disease	1779:1785	arg1	risk					1763:1766	a risk	1761:1766	a risk of serious disease in the vulnerable newborn and mother postpartum	1761:1833	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	11	39	theme	recommended	1844:1854	arg1	prophylaxis					1856:1866	Current recommended prophylaxis	1836:1866	Current recommended prophylaxis	1836:1866	Current recommended prophylaxis consists of administering broad-spectrum antibiotics to GBS-positive mothers during labor.
34986315	0	40	theme	Murine	35:40	arg1	Colonization					72:83	Murine Group B Streptococcus Vaginal Colonization	35:83	Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota	35:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	0	41	theme	B	48:48	arg1	Colonization					72:83	Murine Group B Streptococcus Vaginal Colonization	35:83	Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota	35:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	13	42	theme	targeted	2200:2207	arg1	activity					2223:2230	more targeted antimicrobial activity	2195:2230	more targeted antimicrobial activity	2195:2230	Innovative preventions displaying more targeted antimicrobial activity, while leaving the maternal microbiota intact, are thus appealing.
34986315	5	43	theme	minimal	973:979	arg1	alterations					981:991	minimal alterations	973:991	minimal alterations to the vaginal microbiota	973:1017	HMO treatment reduced GBS vaginal burdens in vivo with minimal alterations to the vaginal microbiota.
34986315	12	44	theme	other	2135:2139	arg1	types					2141:2145	other types	2135:2145	other types of infection	2135:2158	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	12	45	theme	infection	2150:2158	arg1	types					2141:2145	other types	2135:2145	other types of infection	2135:2158	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	5	46	theme	GBS	940:942	arg1	burdens					952:958	GBS vaginal burdens	940:958	GBS vaginal burdens	940:958	HMO treatment reduced GBS vaginal burdens in vivo with minimal alterations to the vaginal microbiota.
34986315	3	47	theme	certain	631:637	arg1	HMOs					639:642	certain HMOs	631:642	certain HMOs	631:642	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	4	48	theme	vaginal	755:761	arg1	model					776:780	a murine vaginal colonization model	746:780	a murine vaginal colonization model	746:780	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	8	49	theme	vaginal	1585:1591	arg1	microenvironment					1593:1608	the vaginal microenvironment	1581:1608	the vaginal microenvironment	1581:1608	We conclude that HMOs are a promising therapeutic bioactive to limit GBS vaginal colonization with minimal impacts on the vaginal microenvironment.
34986315	1	50	theme	women	228:232	arg1	percentage					206:215	a significant percentage	192:215	a significant percentage of healthy women	192:232	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	10	51	dep	newborn	1805:1811	arg1	postpartum					1824:1833	postpartum	1824:1833	postpartum	1824:1833	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	14	52	theme	GBS	2376:2378	arg1	burdens					2380:2386	GBS burdens	2376:2386	GBS burdens	2376:2386	Using a mouse model, we found that human milk oligosaccharides (HMOs) reduce GBS burdens without perturbing the vaginal microbiota.
34986315	2	53	theme	last	337:340	arg1	month					342:346	the last month	333:346	the last month of pregnancy	333:359	Currently, pregnant women are screened in the last month of pregnancy, and GBS-positive women are given antibiotics during parturition to prevent bacterial transmission to the neonate.
34986315	10	54	from	disease	1779:1785	arg1	mother					1817:1822	mother	1817:1822	mother	1817:1822	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	10	54	from	disease	1779:1785	arg1	newborn					1805:1811	newborn	1805:1811	newborn	1805:1811	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	4	55	theme	HMO	807:809	arg1	treatment					811:819	HMO treatment	807:819	HMO treatment	807:819	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	6	56	theme	potent	1035:1040	arg1	activity					1053:1060	potent inhibitory activity	1035:1060	potent inhibitory activity	1035:1060	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	1	57	theme	leading	243:249	arg1	cause					251:255	a leading cause	241:255	a leading cause of neonatal bacterial infections	241:288	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	8	58	theme	minimal	1562:1568	arg1	impacts					1570:1576	minimal impacts	1562:1576	minimal impacts on the vaginal microenvironment	1562:1608	We conclude that HMOs are a promising therapeutic bioactive to limit GBS vaginal colonization with minimal impacts on the vaginal microenvironment.
34986315	10	59	from	newborn	1805:1811	arg1	risk					1763:1766	a risk	1761:1766	a risk of serious disease in the vulnerable newborn and mother postpartum	1761:1833	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	6	60	theme	epithelial	1194:1203	arg1	cells					1205:1209	human vaginal epithelial cells	1180:1209	human vaginal epithelial cells	1180:1209	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	14	61	theme	milk	2340:2343	arg1	HMOs					2363:2366	HMOs	2363:2366	HMOs	2363:2366	Using a mouse model, we found that human milk oligosaccharides (HMOs) reduce GBS burdens without perturbing the vaginal microbiota.
34986315	14	61	theme	milk	2340:2343	arg1	oligosaccharides					2345:2360	human milk oligosaccharides	2334:2360	human milk oligosaccharides (HMOs)	2334:2367	Using a mouse model, we found that human milk oligosaccharides (HMOs) reduce GBS burdens without perturbing the vaginal microbiota.
34986315	2	62	theme	pregnant	302:309	arg1	women					311:315	pregnant women	302:315	pregnant women	302:315	Currently, pregnant women are screened in the last month of pregnancy, and GBS-positive women are given antibiotics during parturition to prevent bacterial transmission to the neonate.
34986315	1	63	theme	vaginal	174:180	arg1	mucosa					182:187	the vaginal mucosa	170:187	the vaginal mucosa of a significant percentage of healthy women	170:232	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	10	64	from	mother	1817:1822	arg1	risk					1763:1766	a risk	1761:1766	a risk of serious disease in the vulnerable newborn and mother postpartum	1761:1833	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	12	65	theme	increased	2116:2124	arg1	risk					2126:2129	increased risk	2116:2129	increased risk for other types of infection	2116:2158	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	9	66	theme	GBS	1640:1642	arg1	ascension					1644:1652	GBS ascension	1640:1652	GBS ascension	1640:1652	IMPORTANCE During pregnancy, GBS ascension into the uterus can cause fetal infection or preterm birth.
34986315	9	66	theme	GBS	1640:1642	arg1	pregnancy					1629:1637	pregnancy	1629:1637	pregnancy	1629:1637	IMPORTANCE During pregnancy, GBS ascension into the uterus can cause fetal infection or preterm birth.
34986315	1	67	theme	significant	194:204	arg1	percentage					206:215	a significant percentage	192:215	a significant percentage of healthy women	192:232	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	4	68	theme	endogenous	863:872	arg1	microbiota					874:883	the endogenous microbiota	859:883	the endogenous microbiota	859:883	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	12	69	theme	neonatal	2090:2097	arg1	bacteria					2103:2110	altered neonatal gut bacteria	2082:2110	altered neonatal gut bacteria	2082:2110	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	8	70	from	impacts	1570:1576	arg1	microenvironment					1593:1608	the vaginal microenvironment	1581:1608	the vaginal microenvironment	1581:1608	We conclude that HMOs are a promising therapeutic bioactive to limit GBS vaginal colonization with minimal impacts on the vaginal microenvironment.
34986315	10	71	theme	GBS	1727:1729	arg1	exposure					1731:1738	GBS exposure	1727:1738	GBS exposure during labor	1727:1751	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	6	72	theme	Lactobacillus	1153:1165	arg1	adherence					1119:1127	adherence	1119:1127	adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells	1119:1209	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	12	73	theme	several	2039:2045	arg1	consequences					2058:2069	several unintended consequences	2039:2069	several unintended consequences	2039:2069	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	12	73	theme	several	2039:2045	arg1	bacteria					2103:2110	altered neonatal gut bacteria	2082:2110	altered neonatal gut bacteria	2082:2110	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	12	73	theme	several	2039:2045	arg1	risk					2126:2129	increased risk	2116:2129	increased risk for other types of infection	2116:2158	Although antibiotics have significantly reduced GBS neonatal disease, there are several unintended consequences, including altered neonatal gut bacteria and increased risk for other types of infection.
34986315	1	74	theme	Group	132:136	arg1	GBS					155:157	GBS	155:157	GBS	155:157	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	1	74	theme	Group	132:136	arg1	Streptococcus					140:152	Group B Streptococcus	132:152	Group B Streptococcus (GBS)	132:158	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	5	75	theme	vaginal	1000:1006	arg1	microbiota					1008:1017	the vaginal microbiota	996:1017	the vaginal microbiota	996:1017	HMO treatment reduced GBS vaginal burdens in vivo with minimal alterations to the vaginal microbiota.
34986315	3	76	theme	biofilm	583:589	arg1	formation					591:599	biofilm formation	583:599	biofilm formation	583:599	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	6	77	theme	GBS	1132:1134	arg1	adherence					1119:1127	adherence	1119:1127	adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells	1119:1209	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	6	78	dep	Lactobacillus	1153:1165	arg1	rhamnosus					1167:1175	Lactobacillus rhamnosus	1153:1175	the probiotic Lactobacillus rhamnosus	1139:1175	HMOs displayed potent inhibitory activity against GBS in vitro, but HMO pretreatment did not alter adherence of GBS or the probiotic Lactobacillus rhamnosus to human vaginal epithelial cells.
34986315	14	79	theme	mouse	2307:2311	arg1	model					2313:2317	a mouse model	2305:2317	a mouse model	2305:2317	Using a mouse model, we found that human milk oligosaccharides (HMOs) reduce GBS burdens without perturbing the vaginal microbiota.
34986315	0	80	theme	Minimal	90:96	arg1	Impact					98:103	Minimal Impact	90:103	Minimal Impact on the Vaginal Microbiota	90:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	13	81	theme	Innovative	2161:2170	arg1	preventions					2172:2182	Innovative preventions	2161:2182	Innovative preventions displaying more targeted antimicrobial activity	2161:2230	Innovative preventions displaying more targeted antimicrobial activity, while leaving the maternal microbiota intact, are thus appealing.
34986315	13	82	dep	appealing	2288:2296	arg1	leaving					2239:2245	leaving	2239:2245	leaving the maternal microbiota intact	2239:2276	Innovative preventions displaying more targeted antimicrobial activity, while leaving the maternal microbiota intact, are thus appealing.
34986315	4	83	theme	human	711:715	arg1	cells					736:740	in vitro human vaginal epithelial cells	702:740	in vitro human vaginal epithelial cells	702:740	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	0	84	theme	Vaginal	112:118	arg1	Microbiota					120:129	the Vaginal Microbiota	108:129	the Vaginal Microbiota	108:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	4	85	from	impact	797:802	arg1	burdens					828:834	GBS burdens	824:834	GBS burdens	824:834	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	4	85	from	impact	797:802	arg1	composition					844:854	the composition	840:854	the composition of the endogenous microbiota	840:883	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	15	86	theme	neonatal	2510:2517	arg1	disease					2519:2525	GBS neonatal disease	2506:2525	GBS neonatal disease	2506:2525	We conclude that HMOs are a promising alternative to antibiotics to reduce GBS neonatal disease.
34986315	5	87	theme	HMO	918:920	arg1	treatment					922:930	HMO treatment	918:930	HMO treatment	918:930	HMO treatment reduced GBS vaginal burdens in vivo with minimal alterations to the vaginal microbiota.
34986315	4	88	theme	rRNA	892:895	arg1	sequencing					906:915	rRNA amplicon sequencing	892:915	rRNA amplicon sequencing	892:915	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	4	89	theme	epithelial	725:734	arg1	cells					736:740	in vitro human vaginal epithelial cells	702:740	in vitro human vaginal epithelial cells	702:740	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	11	90	theme	Current	1836:1842	arg1	prophylaxis					1856:1866	Current recommended prophylaxis	1836:1866	Current recommended prophylaxis	1836:1866	Current recommended prophylaxis consists of administering broad-spectrum antibiotics to GBS-positive mothers during labor.
34986315	0	91	theme	Milk	6:9	arg1	Oligosaccharides					11:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides	0:26	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	7	92	theme	glycosyltransferase	1254:1272	arg1	disruption					1225:1234	disruption	1225:1234	disruption of a putative GBS glycosyltransferase (Δsan_0913)	1225:1284	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	2	93	theme	pregnancy	351:359	arg1	month					342:346	the last month	333:346	the last month of pregnancy	333:359	Currently, pregnant women are screened in the last month of pregnancy, and GBS-positive women are given antibiotics during parturition to prevent bacterial transmission to the neonate.
34986315	1	94	theme	neonatal	260:267	arg1	infections					279:288	neonatal bacterial infections	260:288	neonatal bacterial infections	260:288	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	3	95	attach	isolated	521:528	arg2	HMOs					515:518	HMOs	515:518	HMOs	515:518	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	3	95	attach	isolated	521:528	arg2	oligosaccharides					497:512	human milk oligosaccharides	486:512	human milk oligosaccharides (HMOs) isolated from breastmilk	486:544	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	3	95	attach	isolated	521:528	arg1	breastmilk					535:544	breastmilk	535:544	breastmilk	535:544	Recently, human milk oligosaccharides (HMOs) isolated from breastmilk were found to inhibit GBS growth and biofilm formation in vitro, and women that make certain HMOs are less likely to be vaginally colonized with GBS.
34986315	7	96	theme	putative	1241:1248	arg1	Δsan_0913					1275:1283	Δsan_0913	1275:1283	Δsan_0913	1275:1283	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	7	96	theme	putative	1241:1248	arg1	glycosyltransferase					1254:1272	a putative GBS glycosyltransferase	1239:1272	a putative GBS glycosyltransferase (Δsan_0913)	1239:1284	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	4	97	theme	in	702:703	arg1	cells					736:740	in vitro human vaginal epithelial cells	702:740	in vitro human vaginal epithelial cells	702:740	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	0	98	theme	Group	42:46	arg1	Colonization					72:83	Murine Group B Streptococcus Vaginal Colonization	35:83	Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota	35:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	1	99	theme	infections	279:288	arg1	cause					251:255	a leading cause	241:255	a leading cause of neonatal bacterial infections	241:288	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	10	100	theme	serious	1771:1777	arg1	disease					1779:1785	serious disease	1771:1785	serious disease in the vulnerable newborn and mother postpartum	1771:1833	In addition, GBS exposure during labor creates a risk of serious disease in the vulnerable newborn and mother postpartum.
34986315	4	101	theme	colonization	763:774	arg1	model					776:780	a murine vaginal colonization model	746:780	a murine vaginal colonization model	746:780	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
34986315	0	102	theme	Streptococcus	50:62	arg1	Colonization					72:83	Murine Group B Streptococcus Vaginal Colonization	35:83	Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota	35:129	Human Milk Oligosaccharides Reduce Murine Group B Streptococcus Vaginal Colonization with Minimal Impact on the Vaginal Microbiota.
34986315	7	103	theme	HMOs	1457:1460	arg1	absence					1446:1452	the absence	1442:1452	the absence of HMOs	1442:1460	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	2	104	theme	bacterial	437:445	arg1	transmission					447:458	bacterial transmission	437:458	bacterial transmission to the neonate	437:473	Currently, pregnant women are screened in the last month of pregnancy, and GBS-positive women are given antibiotics during parturition to prevent bacterial transmission to the neonate.
34986315	14	105	theme	vaginal	2411:2417	arg1	microbiota					2419:2428	the vaginal microbiota	2407:2428	the vaginal microbiota	2407:2428	Using a mouse model, we found that human milk oligosaccharides (HMOs) reduce GBS burdens without perturbing the vaginal microbiota.
34986315	13	106	theme	antimicrobial	2209:2221	arg1	activity					2223:2230	more targeted antimicrobial activity	2195:2230	more targeted antimicrobial activity	2195:2230	Innovative preventions displaying more targeted antimicrobial activity, while leaving the maternal microbiota intact, are thus appealing.
34986315	7	107	theme	HMO	1330:1332	arg1	inhibition					1334:1343	HMO inhibition	1330:1343	HMO inhibition	1330:1343	In addition, disruption of a putative GBS glycosyltransferase (Δsan_0913) rendered the bacterium largely resistant to HMO inhibition in vitro and in vivo but did not compromise its adherence, colonization, or biofilm formation in the absence of HMOs.
34986315	1	108	theme	healthy	220:226	arg1	women					228:232	healthy women	220:232	healthy women	220:232	Group B Streptococcus (GBS) colonizes the vaginal mucosa of a significant percentage of healthy women and is a leading cause of neonatal bacterial infections.
34986315	5	109	theme	vaginal	944:950	arg1	burdens					952:958	GBS vaginal burdens	940:958	GBS vaginal burdens	940:958	HMO treatment reduced GBS vaginal burdens in vivo with minimal alterations to the vaginal microbiota.
34986315	8	110	theme	vaginal	1536:1542	arg1	colonization					1544:1555	GBS vaginal colonization	1532:1555	GBS vaginal colonization with minimal impacts on the vaginal microenvironment	1532:1608	We conclude that HMOs are a promising therapeutic bioactive to limit GBS vaginal colonization with minimal impacts on the vaginal microenvironment.
34986315	4	111	theme	murine	748:753	arg1	model					776:780	a murine vaginal colonization model	746:780	a murine vaginal colonization model	746:780	Using in vitro human vaginal epithelial cells and a murine vaginal colonization model, we tested the impact of HMO treatment on GBS burdens and the composition of the endogenous microbiota by 16S rRNA amplicon sequencing.
37385587	3	0	theme	Muc2	315:318	arg1	degradation					320:330	a bacteria-dependent Muc2 degradation	294:330	a bacteria-dependent Muc2 degradation	294:330	Among various glycosylation patterns of Muc2, sialylation inhibits a bacteria-dependent Muc2 degradation.
37385587	7	1	with	Mice	892:895	arg1	mutation					912:919	a B3galt5 mutation	902:919	a B3galt5 mutation associated with inflammatory bowel disease (IBD)	902:968	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	5	2	theme	Muc2	656:659	arg1	structure					643:651	the network structure	631:651	the network structure of Muc2	631:659	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	6	3	theme	mice	731:734	arg1	mucus					722:726	The colonic mucus	710:726	The colonic mucus of mice lacking St6galnac6 and B3galt5	710:765	The colonic mucus of mice lacking St6galnac6 and B3galt5 was less sialylated, thinner, and more permeable to microbiota, resulting in high susceptibility to intestinal inflammation.
37385587	1	4	theme	network	123:129	arg1	structure					131:139	a network structure	121:139	a network structure	121:139	In the intestine, Mucin 2 (Muc2) forms a network structure and prevents bacterial invasion.
37385587	9	5	theme	polymers	1403:1410	arg1	crosslinking					1382:1393	crosslinking	1382:1393	crosslinking of Muc2 polymers to form the network structure	1382:1440	Thus, sialylation mediates the negative charging of Muc2 and facilitates crosslinking of Muc2 polymers to form the network structure, and thereby inhibits bacterial invasion in the colon to maintain gut homeostasis.
37385587	8	6	theme	reduced	1195:1201	arg1	sialylation					1203:1213	reduced sialylation	1195:1213	reduced sialylation	1195:1213	In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded.
37385587	7	7	theme	Muc2	1123:1126	arg1	sialylation					1108:1118	the reduced sialylation	1096:1118	the reduced sialylation of Muc2	1096:1126	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	4	8	dep	structure	391:399	arg1	prevents					417:424	prevents	417:424	prevents the mucin degradation	417:446	However, the mechanisms by which Muc2 creates the network structure and sialylation prevents the mucin degradation remain unknown.
37385587	4	8	dep	structure	391:399	arg1	the					379:381	the	379:381	the	379:381	However, the mechanisms by which Muc2 creates the network structure and sialylation prevents the mucin degradation remain unknown.
37385587	5	9	gly	disialylated	578:589	arg1	glycans					591:597	disialylated glycans	578:597	disialylated glycans	578:597	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	9	10	theme	gut	1508:1510	arg1	homeostasis					1512:1522	gut homeostasis	1508:1522	gut homeostasis	1508:1522	Thus, sialylation mediates the negative charging of Muc2 and facilitates crosslinking of Muc2 polymers to form the network structure, and thereby inhibits bacterial invasion in the colon to maintain gut homeostasis.
37385587	4	11	theme	network	383:389	arg1	structure					391:399	network structure	383:399	network structure	383:399	However, the mechanisms by which Muc2 creates the network structure and sialylation prevents the mucin degradation remain unknown.
37385587	5	12	theme	negative	674:681	arg1	charge					683:688	negative charge	674:688	negative charge	674:688	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	3	13	theme	various	233:239	arg1	patterns					255:262	various glycosylation patterns	233:262	various glycosylation patterns of Muc2	233:270	Among various glycosylation patterns of Muc2, sialylation inhibits a bacteria-dependent Muc2 degradation.
37385587	5	14	theme	colon-specific	489:502	arg1	B3galt5					541:547	B3galt5	541:547	B3galt5	541:547	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	5	14	theme	colon-specific	489:502	arg1	St6galnac6					526:535	St6galnac6	526:535	St6galnac6	526:535	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	5	14	theme	colon-specific	489:502	arg1	glycosyltransferases					504:523	two colon-specific glycosyltransferases	485:523	two colon-specific glycosyltransferases	485:523	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	7	15	gly	sialylation	1108:1118	arg1	Muc2					1123:1126	Muc2	1123:1126	Muc2	1123:1126	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	3	16	theme	glycosylation	241:253	arg1	patterns					255:262	various glycosylation patterns	233:262	various glycosylation patterns of Muc2	233:270	Among various glycosylation patterns of Muc2, sialylation inhibits a bacteria-dependent Muc2 degradation.
37385587	5	17	theme	disialylated	578:589	arg1	glycans					591:597	disialylated glycans	578:597	disialylated glycans	578:597	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	6	18	theme	colonic	714:720	arg1	mucus					722:726	The colonic mucus	710:726	The colonic mucus of mice lacking St6galnac6 and B3galt5	710:765	The colonic mucus of mice lacking St6galnac6 and B3galt5 was less sialylated, thinner, and more permeable to microbiota, resulting in high susceptibility to intestinal inflammation.
37385587	3	19	gly	glycosylation	241:253	arg1	Muc2					267:270	Muc2	267:270	Muc2	267:270	Among various glycosylation patterns of Muc2, sialylation inhibits a bacteria-dependent Muc2 degradation.
37385587	5	20	theme	glycans	591:597	arg1	generation					564:573	the generation	560:573	the generation of disialylated glycans	560:597	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	1	21	theme	bacterial	154:162	arg1	invasion					164:171	bacterial invasion	154:171	bacterial invasion	154:171	In the intestine, Mucin 2 (Muc2) forms a network structure and prevents bacterial invasion.
37385587	7	22	gly	disialylated	994:1005	arg1	glycans					1007:1013	disialylated glycans	994:1013	disialylated glycans of mucus	994:1022	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	7	23	theme	inflammatory	937:948	arg1	disease					956:962	inflammatory bowel disease	937:962	inflammatory bowel disease (IBD)	937:968	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	7	23	theme	inflammatory	937:948	arg1	IBD					965:967	IBD	965:967	IBD	965:967	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	7	24	theme	reduced	1100:1106	arg1	sialylation					1108:1118	the reduced sialylation	1096:1118	the reduced sialylation of Muc2	1096:1126	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	7	25	theme	IBD	1167:1169	arg1	pathogenesis					1151:1162	the pathogenesis	1147:1162	the pathogenesis of IBD	1147:1169	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	3	26	theme	Muc2	267:270	arg1	patterns					255:262	various glycosylation patterns	233:262	various glycosylation patterns of Muc2	233:270	Among various glycosylation patterns of Muc2, sialylation inhibits a bacteria-dependent Muc2 degradation.
37385587	7	27	theme	bowel	950:954	arg1	disease					956:962	inflammatory bowel disease	937:962	inflammatory bowel disease (IBD)	937:968	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	7	27	theme	bowel	950:954	arg1	IBD					965:967	IBD	965:967	IBD	965:967	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	0	28	theme	mucus	19:23	arg1	architecture					25:36	mucus architecture	19:36	mucus architecture inhibiting bacterial invasion in the colon	19:79	Sialylation shapes mucus architecture inhibiting bacterial invasion in the colon.
37385587	8	29	with	mice	1185:1188	arg1	sialylation					1203:1213	reduced sialylation	1195:1213	reduced sialylation	1195:1213	In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded.
37385587	8	30	theme	network	1249:1255	arg1	structure					1257:1265	the network structure	1245:1265	the network structure	1245:1265	In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded.
37385587	0	31	theme	bacterial	49:57	arg1	invasion					59:66	bacterial invasion	49:66	bacterial invasion	49:66	Sialylation shapes mucus architecture inhibiting bacterial invasion in the colon.
37385587	9	32	theme	negative	1340:1347	arg1	charging					1349:1356	the negative charging	1336:1356	the negative charging of Muc2	1336:1364	Thus, sialylation mediates the negative charging of Muc2 and facilitates crosslinking of Muc2 polymers to form the network structure, and thereby inhibits bacterial invasion in the colon to maintain gut homeostasis.
37385587	7	33	theme	intestinal	1055:1064	arg1	inflammation					1066:1077	intestinal inflammation	1055:1077	intestinal inflammation	1055:1077	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	6	34	theme	intestinal	867:876	arg1	inflammation					878:889	intestinal inflammation	867:889	intestinal inflammation	867:889	The colonic mucus of mice lacking St6galnac6 and B3galt5 was less sialylated, thinner, and more permeable to microbiota, resulting in high susceptibility to intestinal inflammation.
37385587	9	35	theme	bacterial	1464:1472	arg1	invasion					1474:1481	bacterial invasion	1464:1481	bacterial invasion	1464:1481	Thus, sialylation mediates the negative charging of Muc2 and facilitates crosslinking of Muc2 polymers to form the network structure, and thereby inhibits bacterial invasion in the colon to maintain gut homeostasis.
37385587	9	36	theme	network	1424:1430	arg1	structure					1432:1440	the network structure	1420:1440	the network structure	1420:1440	Thus, sialylation mediates the negative charging of Muc2 and facilitates crosslinking of Muc2 polymers to form the network structure, and thereby inhibits bacterial invasion in the colon to maintain gut homeostasis.
37385587	6	37	gly	sialylated	776:785	arg1	mucus					722:726	The colonic mucus	710:726	The colonic mucus of mice lacking St6galnac6 and B3galt5	710:765	The colonic mucus of mice lacking St6galnac6 and B3galt5 was less sialylated, thinner, and more permeable to microbiota, resulting in high susceptibility to intestinal inflammation.
37385587	2	38	theme	barrier	209:215	arg1	function					217:224	Muc2 barrier function	204:224	Muc2 barrier function	204:224	Glycans are indispensable for Muc2 barrier function.
37385587	7	39	theme	mucus	1018:1022	arg1	glycans					1007:1013	disialylated glycans	994:1013	disialylated glycans of mucus	994:1022	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	2	40	theme	Muc2	204:207	arg1	function					217:224	Muc2 barrier function	204:224	Muc2 barrier function	204:224	Glycans are indispensable for Muc2 barrier function.
37385587	8	41	theme	negative	1216:1223	arg1	charge					1225:1230	negative charge	1216:1230	negative charge	1216:1230	In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded.
37385587	9	42	theme	Muc2	1361:1364	arg1	charging					1349:1356	the negative charging	1336:1356	the negative charging of Muc2	1336:1364	Thus, sialylation mediates the negative charging of Muc2 and facilitates crosslinking of Muc2 polymers to form the network structure, and thereby inhibits bacterial invasion in the colon to maintain gut homeostasis.
37385587	3	43	theme	bacteria-dependent	296:313	arg1	degradation					320:330	a bacteria-dependent Muc2 degradation	294:330	a bacteria-dependent Muc2 degradation	294:330	Among various glycosylation patterns of Muc2, sialylation inhibits a bacteria-dependent Muc2 degradation.
37385587	6	44	theme	high	844:847	arg1	susceptibility					849:862	high susceptibility	844:862	high susceptibility to intestinal inflammation	844:889	The colonic mucus of mice lacking St6galnac6 and B3galt5 was less sialylated, thinner, and more permeable to microbiota, resulting in high susceptibility to intestinal inflammation.
37385587	5	45	theme	network	635:641	arg1	structure					643:651	the network structure	631:651	the network structure of Muc2	631:659	Here, by focusing on two colon-specific glycosyltransferases, St6galnac6 and B3galt5, mediating the generation of disialylated glycans, we show that sialylation forms the network structure of Muc2 by providing negative charge and hydrophilicity.
37385587	7	46	theme	glycans	1007:1013	arg1	susceptibility					1037:1050	the high susceptibility	1028:1050	the high susceptibility to intestinal inflammation	1028:1077	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	7	46	theme	glycans	1007:1013	arg1	loss					986:989	the loss	982:989	the loss of disialylated glycans of mucus	982:1022	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	8	47	gly	sialylation	1203:1213	arg1	mice					1185:1188	mice	1185:1188	mice with reduced sialylation	1185:1213	In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded.
37385587	7	48	theme	high	1032:1035	arg1	susceptibility					1037:1050	the high susceptibility	1028:1050	the high susceptibility to intestinal inflammation	1028:1077	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	8	49	theme	many	1286:1289	arg1	bacteria					1291:1298	many bacteria	1286:1298	many bacteria	1286:1298	In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded.
37385587	7	50	theme	B3galt5	904:910	arg1	mutation					912:919	a B3galt5 mutation	902:919	a B3galt5 mutation associated with inflammatory bowel disease (IBD)	902:968	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	4	51	theme	mucin	430:434	arg1	degradation					436:446	the mucin degradation	426:446	the mucin degradation	426:446	However, the mechanisms by which Muc2 creates the network structure and sialylation prevents the mucin degradation remain unknown.
37385587	7	52	theme	disialylated	994:1005	arg1	glycans					1007:1013	disialylated glycans	994:1013	disialylated glycans of mucus	994:1022	Mice with a B3galt5 mutation associated with inflammatory bowel disease (IBD) also showed the loss of disialylated glycans of mucus and the high susceptibility to intestinal inflammation, suggesting that the reduced sialylation of Muc2 is associated with the pathogenesis of IBD.
37385587	6	53	dep	sialylated	776:785	arg1	less					771:774	less	771:774	less	771:774	The colonic mucus of mice lacking St6galnac6 and B3galt5 was less sialylated, thinner, and more permeable to microbiota, resulting in high susceptibility to intestinal inflammation.
37385587	8	54	theme	mice	1185:1188	arg1	mucins					1175:1180	mucins	1175:1180	mucins of mice with reduced sialylation	1175:1213	In mucins of mice with reduced sialylation, negative charge was reduced, the network structure was disturbed, and many bacteria invaded.
37385587	9	55	theme	Muc2	1398:1401	arg1	polymers					1403:1410	Muc2 polymers	1398:1410	Muc2 polymers	1398:1410	Thus, sialylation mediates the negative charging of Muc2 and facilitates crosslinking of Muc2 polymers to form the network structure, and thereby inhibits bacterial invasion in the colon to maintain gut homeostasis.
36349588	0	0	theme	regium	79:84	arg1	Pilg					96:99	ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg	36:99	Pilg	96:99	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	4	1	theme	excision	755:762	arg1	model					770:774	excision wound model	755:774	excision wound model	755:774	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	6	2	from	extravasation	1002:1014	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	6	3	theme	protein	994:1000	arg1	extravasation					1002:1014	protein extravasation	994:1014	protein extravasation	994:1014	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	4	4	theme	carrageenan-induced	672:690	arg1	pleurisy					692:699	carrageenan-induced pleurisy	672:699	carrageenan-induced pleurisy	672:699	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	6	5	from	closure	1142:1148	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	0	6	theme	Cochlospermum	65:77	arg1	Pilg					96:99	ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg	36:99	Pilg	96:99	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	2	7	theme	skin	317:320	arg1	disorders					322:330	skin disorders	317:330	skin disorders	317:330	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	0	8	theme	Schrank	87:93	arg1	Pilg					96:99	ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg	36:99	Pilg	96:99	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	7	9	theme	anti-nociceptive	1243:1258	arg1	potential					1279:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential	1220:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium	1220:1314	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	6	10	from	hypersensitivity	1105:1120	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	7	11	theme	plant	1369:1373	arg1	effect					1354:1359	the potential therapeutic effect	1328:1359	the potential therapeutic effect of this plant	1328:1373	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	4	12	theme	wound	764:768	arg1	model					770:774	excision wound model	755:774	excision wound model	755:774	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	6	13	from	production	982:991	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	3	14	dep	regium	440:445	arg1	leaves					447:452	leaves	447:452	leaves	447:452	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	6	15	from	regeneration	1161:1172	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	0	16	theme	Pilg	96:99	arg1	effects					25:31	effects	25:31	effects	25:31	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	0	16	theme	Pilg	96:99	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	4	17	theme	pain	739:742	arg1	model					744:748	formalin-induced pain model	722:748	formalin-induced pain model	722:748	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	5	18	from	RESULTS	777:783	arg1	total					788:792	total	788:792	total	788:792	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	5	18	from	RESULTS	777:783	arg1	compounds					798:806	25 compounds	795:806	25 compounds	795:806	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	5	18	from	RESULTS	777:783	arg1	quercitrin					819:828	quercitrin	819:828	quercitrin	819:828	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	5	18	from	RESULTS	777:783	arg1	gallate					838:844	methyl gallate	831:844	methyl gallate	831:844	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	5	18	from	RESULTS	777:783	arg1	1,2,3,4,6-pentagalloylhexose					851:878	1,2,3,4,6-pentagalloylhexose	851:878	1,2,3,4,6-pentagalloylhexose	851:878	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	7	19	dep	CONCLUSIONS	1175:1185	arg1	established					1208:1218	established	1208:1218	established	1208:1218	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	7	20	theme	anti-inflammatory	1224:1240	arg1	potential					1279:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential	1220:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium	1220:1314	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	4	21	theme	paw	705:707	arg1	edema					709:713	paw edema	705:713	paw edema	705:713	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	3	22	theme	phytochemical	366:378	arg1	constituents					380:391	the phytochemical constituents	362:391	the phytochemical constituents	362:391	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	2	23	theme	Brazilian	247:255	arg1	medicine					257:264	folk Brazilian medicine	242:264	folk Brazilian medicine	242:264	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	2	24	dep	cerrado	230:236	arg1	do					227:228	do	227:228	do	227:228	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	3	25	theme	STUDY	344:348	arg1	AIM					333:335	AIM	333:335	AIM OF THE STUDY	333:348	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	5	26	with	compounds	798:806	arg1	detectability					894:906	highest detectability	886:906	highest detectability	886:906	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	7	27	theme	present	1191:1197	arg1	findings					1199:1206	The present findings	1187:1206	The present findings	1187:1206	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	2	28	theme	fight	282:286	arg1	infections					288:297	fight infections	282:297	fight infections	282:297	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	6	29	theme	nitric	969:974	arg1	production					982:991	nitric oxide production	969:991	nitric oxide production	969:991	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	0	30	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	6	31	theme	tissue	1154:1159	arg1	regeneration					1161:1172	tissue regeneration	1154:1172	tissue regeneration	1154:1172	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	6	32	theme	wound	1136:1140	arg1	closure					1142:1148	improved wound closure	1127:1148	improved wound closure	1127:1148	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	6	33	from	migration	958:966	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	3	34	theme	C.	506:507	arg1	GEECR					521:525	C. regium gel (GEECR)	506:526	C. regium gel (GEECR)	506:526	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	2	35	from	"	237:237	arg1	medicine					257:264	folk Brazilian medicine	242:264	folk Brazilian medicine	242:264	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	7	36	theme	therapeutic	1342:1352	arg1	effect					1354:1359	the potential therapeutic effect	1328:1359	the potential therapeutic effect of this plant	1328:1373	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	6	37	theme	oxide	976:980	arg1	production					982:991	nitric oxide production	969:991	nitric oxide production	969:991	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	7	38	theme	wound	1265:1269	arg1	healing					1271:1277	wound healing	1265:1277	wound healing	1265:1277	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	6	39	theme	improved	1127:1134	arg1	closure					1142:1148	improved wound closure	1127:1148	improved wound closure	1127:1148	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	3	40	theme	extract	426:432	arg1	effects					401:407	the effects	397:407	the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain	397:484	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	3	40	theme	extract	426:432	arg1	effects					495:501	the effects	491:501	the effects of C. regium gel (GEECR) on wound healing	491:543	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	6	41	from	pain	1049:1052	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	5	42	theme	methyl	831:836	arg1	gallate					838:844	methyl gallate	831:844	methyl gallate	831:844	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	6	43	theme	leukocyte	948:956	arg1	migration					958:966	leukocyte migration	948:966	leukocyte migration	948:966	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	0	44	theme	ethanolic	36:44	arg1	extract					46:52	ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg	36:99	extract	46:52	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	2	45	theme	ETHNOPHARMACOLOGICAL	144:163	arg1	RELEVANCE					165:173	ETHNOPHARMACOLOGICAL RELEVANCE	144:173	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium	144:194	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	4	46	theme	edema	709:713	arg1	tests					715:719	carrageenan-induced pleurisy and paw edema tests	672:719	carrageenan-induced pleurisy and paw edema tests	672:719	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	4	47	theme	METHODS	560:566	arg1	Animals					568:574	MATERIALS AND METHODS Animals	546:574	MATERIALS AND METHODS Animals	546:574	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	6	48	dep	pain	1049:1052	arg1	inflammatory					1085:1096	inflammatory	1085:1096	inflammatory	1085:1096	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	6	48	dep	pain	1049:1052	arg1	neurogenic					1070:1079	neurogenic	1070:1079	neurogenic	1070:1079	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	2	49	theme	Algodãozinho	214:225	arg1	cerrado					230:236	Algodãozinho do cerrado	214:236	"Algodãozinho do cerrado" in folk Brazilian medicine	213:264	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	7	50	theme	the	1292:1294	arg1	potential					1279:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential	1220:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium	1220:1314	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	3	51	theme	ethanolic	416:424	arg1	extract					426:432	the ethanolic extract	412:432	the ethanolic extract of C. regium leaves (EECR)	412:459	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	3	52	theme	regium	509:514	arg1	GEECR					521:525	C. regium gel (GEECR)	506:526	C. regium gel (GEECR)	506:526	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	6	53	from	edema	1017:1021	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	0	54	theme	extract	46:52	arg1	effects					25:31	effects	25:31	effects	25:31	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	0	54	theme	extract	46:52	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	4	55	theme	formalin-induced	722:737	arg1	model					744:748	formalin-induced pain model	722:748	formalin-induced pain model	722:748	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	3	56	theme	gel	516:518	arg1	GEECR					521:525	C. regium gel (GEECR)	506:526	C. regium gel (GEECR)	506:526	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	6	57	from	hyperalgesia	1035:1046	arg1	phases					1062:1067	both phases	1057:1067	both phases	1057:1067	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	6	58	theme	mechanical	1024:1033	arg1	hyperalgesia					1035:1046	mechanical hyperalgesia	1024:1046	mechanical hyperalgesia	1024:1046	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36349588	4	59	theme	pleurisy	692:699	arg1	tests					715:719	carrageenan-induced pleurisy and paw edema tests	672:719	carrageenan-induced pleurisy and paw edema tests	672:719	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	3	60	theme	wound	531:535	arg1	healing					537:543	wound healing	531:543	wound healing	531:543	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	2	61	theme	folk	242:245	arg1	medicine					257:264	folk Brazilian medicine	242:264	folk Brazilian medicine	242:264	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	3	62	theme	GEECR	521:525	arg1	effects					401:407	the effects	397:407	the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain	397:484	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	3	62	theme	GEECR	521:525	arg1	effects					495:501	the effects	491:501	the effects of C. regium gel (GEECR) on wound healing	491:543	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	2	63	used	used	274:277	arg2	RELEVANCE					165:173	ETHNOPHARMACOLOGICAL RELEVANCE	144:173	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium	144:194	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	7	64	theme	potential	1332:1340	arg1	effect					1354:1359	the potential therapeutic effect	1328:1359	the potential therapeutic effect of this plant	1328:1373	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	7	65	theme	healing	1271:1277	arg1	potential					1279:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential	1220:1287	the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium	1220:1314	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	3	66	theme	regium	440:445	arg1	extract					426:432	the ethanolic extract	412:432	the ethanolic extract of C. regium leaves (EECR)	412:459	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	7	67	dep	the	1292:1294	arg1	leaves					1296:1301	leaves	1296:1301	leaves	1296:1301	CONCLUSIONS The present findings established the anti-inflammatory, anti-nociceptive, and wound healing potential of the leaves of C. regium, confirming the potential therapeutic effect of this plant.
36349588	2	68	dep	Cochlospermum	175:187	arg1	regium					189:194	regium	189:194	regium	189:194	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	0	69	theme	of	62:63	arg1	Pilg					96:99	ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg	36:99	Pilg	96:99	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	2	70	theme	Cochlospermum	175:187	arg1	RELEVANCE					165:173	ETHNOPHARMACOLOGICAL RELEVANCE	144:173	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium	144:194	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	5	71	theme	highest	886:892	arg1	detectability					894:906	highest detectability	886:906	highest detectability	886:906	RESULTS In total, 25 compounds, including quercitrin, methyl gallate, and 1,2,3,4,6-pentagalloylhexose, with highest detectability were identified.
36349588	4	72	theme	MATERIALS	546:554	arg1	Animals					568:574	MATERIALS AND METHODS Animals	546:574	MATERIALS AND METHODS Animals	546:574	MATERIALS AND METHODS Animals were treated with EECR (30-300 mg/kg) or GEECR (1.25 and 2.5%) and studies were conducted using carrageenan-induced pleurisy and paw edema tests, formalin-induced pain model, and excision wound model.
36349588	3	73	from	effects	495:501	arg1	inflammation					464:475	inflammation	464:475	inflammation	464:475	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	3	73	from	effects	495:501	arg1	pain					481:484	pain	481:484	pain	481:484	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	3	73	from	effects	495:501	arg1	healing					537:543	wound healing	531:543	wound healing	531:543	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	0	74	theme	gel	58:60	arg1	Pilg					96:99	ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg	36:99	Pilg	96:99	Chemical composition and effects of ethanolic extract and gel of Cochlospermum regium (Schrank) Pilg.
36349588	2	75	theme	cerrado	230:236	arg1	"					237:237	"Algodãozinho do cerrado"	213:237	"Algodãozinho do cerrado" in folk Brazilian medicine	213:264	ETHNOPHARMACOLOGICAL RELEVANCE Cochlospermum regium is well-known as "Algodãozinho do cerrado" in folk Brazilian medicine, and is used to fight infections, inflammation and skin disorders.
36349588	3	76	from	effects	401:407	arg1	healing					537:543	wound healing	531:543	wound healing	531:543	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	3	76	from	effects	401:407	arg1	pain					481:484	pain	481:484	pain	481:484	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	3	76	from	effects	401:407	arg1	inflammation					464:475	inflammation	464:475	inflammation	464:475	AIM OF THE STUDY To identify the phytochemical constituents and the effects of the ethanolic extract of C. regium leaves (EECR) on inflammation and pain, and the effects of C. regium gel (GEECR) on wound healing.
36349588	6	77	theme	cold	1100:1103	arg1	hypersensitivity					1105:1120	cold hypersensitivity	1100:1120	cold hypersensitivity	1100:1120	The treatments reduced leukocyte migration, nitric oxide production, protein extravasation, edema, mechanical hyperalgesia, pain in both phases (neurogenic and inflammatory), cold hypersensitivity, and improved wound closure and tissue regeneration.
36656954	4	0	theme	intracellular	707:719	arg1	domains					728:734	the intracellular kinase domains	703:734	the intracellular kinase domains whereas infection requires functional ectodomains	703:784	We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains.
36656954	5	1	theme	distant	823:829	arg1	receptors					838:846	evolutionarily distant barley receptors	808:846	evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals	808:963	Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
36656954	3	2	theme	core	515:518	arg1	complex					529:535	the core receptor complex	511:535	the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection	511:648	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	2	3	with	symbiosis	391:399	arg1	bacteria					422:429	nitrogen-fixing bacteria	406:429	nitrogen-fixing bacteria	406:429	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	1	4	from	challenge	173:181	arg1	biology					186:192	biology	186:192	biology	186:192	Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology.
36656954	3	5	theme	cortical	552:559	arg1	organogenesis					573:585	the cortical root nodule organogenesis	548:585	the cortical root nodule organogenesis program as well as the epidermal program controlling infection	548:648	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	6	theme	root	561:564	arg1	organogenesis					573:585	the cortical root nodule organogenesis	548:585	the cortical root nodule organogenesis program as well as the epidermal program controlling infection	548:648	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	4	7	theme	kinase	721:726	arg1	domains					728:734	the intracellular kinase domains	703:734	the intracellular kinase domains whereas infection requires functional ectodomains	703:784	We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains.
36656954	0	8	from	complex	52:58	arg1	symbiosis					75:83	root nodule symbiosis	63:83	root nodule symbiosis	63:83	Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
36656954	5	9	theme	nitrogen-fixation	934:950	arg1	engineering					908:918	engineering	908:918	engineering of biological nitrogen-fixation into cereals	908:963	Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
36656954	5	10	theme	barley	831:836	arg1	receptors					838:846	evolutionarily distant barley receptors	808:846	evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals	808:963	Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
36656954	5	11	theme	nodule	867:872	arg1	organogenesis					874:886	root nodule organogenesis	862:886	root nodule organogenesis	862:886	Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
36656954	5	12	theme	biological	923:932	arg1	nitrogen-fixation					934:950	biological nitrogen-fixation	923:950	biological nitrogen-fixation	923:950	Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
36656954	5	13	theme	root	862:865	arg1	organogenesis					874:886	root nodule organogenesis	862:886	root nodule organogenesis	862:886	Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
36656954	2	14	theme	receptors	315:323	arg1	activation					288:297	activation	288:297	activation	288:297	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	2	14	theme	receptors	315:323	arg1	assembly					275:282	assembly	275:282	assembly	275:282	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	2	15	theme	plant	385:389	arg1	symbiosis					391:399	plant symbiosis	385:399	plant symbiosis with nitrogen-fixing bacteria	385:429	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	0	16	theme	Nanobody-driven	0:14	arg1	signaling					16:24	Nanobody-driven signaling	0:24	Nanobody-driven signaling	0:24	Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
36656954	2	17	theme	surface	307:313	arg1	receptors					315:323	cell surface receptors	302:323	cell surface receptors	302:323	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	2	18	theme	cell	302:305	arg1	receptors					315:323	cell surface receptors	302:323	cell surface receptors	302:323	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	3	19	theme	factor	469:474	arg1	NFR5					495:498	NFR5	495:498	NFR5	495:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	19	theme	factor	469:474	arg1	NFR1					486:489	NFR1	486:489	NFR1	486:489	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	19	theme	factor	469:474	arg1	receptors					476:484	the Lotus japonicus Nod factor receptors	445:484	the Lotus japonicus Nod factor receptors NFR1 and NFR5	445:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	20	theme	organogenesis	573:585	arg1	program					587:593	the cortical root nodule organogenesis program	548:593	the cortical root nodule organogenesis program as well as the epidermal program controlling infection	548:648	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	21	theme	receptor	520:527	arg1	complex					529:535	the core receptor complex	511:535	the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection	511:648	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	0	22	theme	receptor	43:50	arg1	complex					52:58	the core receptor complex	34:58	the core receptor complex in root nodule symbiosis	34:83	Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
36656954	4	23	theme	organogenesis	664:676	arg1	signaling					678:686	organogenesis signaling	664:686	organogenesis signaling	664:686	We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains.
36656954	3	24	theme	japonicus	455:463	arg1	NFR5					495:498	NFR5	495:498	NFR5	495:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	24	theme	japonicus	455:463	arg1	NFR1					486:489	NFR1	486:489	NFR1	486:489	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	24	theme	japonicus	455:463	arg1	receptors					476:484	the Lotus japonicus Nod factor receptors	445:484	the Lotus japonicus Nod factor receptors NFR1 and NFR5	445:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	0	25	theme	core	38:41	arg1	complex					52:58	the core receptor complex	34:58	the core receptor complex in root nodule symbiosis	34:83	Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
36656954	2	26	theme	synthetic	227:235	arg1	approach					237:244	a synthetic approach	225:244	a synthetic approach based on nanobodies	225:264	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	2	27	theme	nitrogen-fixing	406:420	arg1	bacteria					422:429	nitrogen-fixing bacteria	406:429	nitrogen-fixing bacteria	406:429	To address this, we developed a synthetic approach based on nanobodies to drive assembly and activation of cell surface receptors and apply the concept by manipulating receptors that govern plant symbiosis with nitrogen-fixing bacteria.
36656954	5	28	dep	activate	853:860	arg1	enable					901:906	enable	901:906	could enable engineering of biological nitrogen-fixation into cereals	895:963	Finally, we identify evolutionarily distant barley receptors that activate root nodule organogenesis, which could enable engineering of biological nitrogen-fixation into cereals.
36656954	1	29	theme	complexes	158:166	arg1	activation					120:129	activation	120:129	activation	120:129	Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology.
36656954	1	29	theme	complexes	158:166	arg1	composition					104:114	composition	104:114	composition	104:114	Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology.
36656954	3	30	theme	epidermal	610:618	arg1	program					620:626	the epidermal program	606:626	the cortical root nodule organogenesis program as well as the epidermal program controlling infection	548:648	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	1	31	theme	multicomponent	134:147	arg1	complexes					158:166	multicomponent receptor complexes	134:166	multicomponent receptor complexes	134:166	Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology.
36656954	3	32	theme	nodule	566:571	arg1	organogenesis					573:585	the cortical root nodule organogenesis	548:585	the cortical root nodule organogenesis program as well as the epidermal program controlling infection	548:648	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	4	33	dep	domains	728:734	arg1	requires					754:761	requires	754:761	requires functional ectodomains	754:784	We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains.
36656954	1	34	theme	receptor	149:156	arg1	complexes					158:166	multicomponent receptor complexes	134:166	multicomponent receptor complexes	134:166	Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology.
36656954	4	35	theme	functional	763:772	arg1	ectodomains					774:784	functional ectodomains	763:784	functional ectodomains	763:784	We find that organogenesis signaling is mediated by the intracellular kinase domains whereas infection requires functional ectodomains.
36656954	0	36	theme	nodule	68:73	arg1	symbiosis					75:83	root nodule symbiosis	63:83	root nodule symbiosis	63:83	Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
36656954	3	37	theme	Nod	465:467	arg1	NFR5					495:498	NFR5	495:498	NFR5	495:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	37	theme	Nod	465:467	arg1	NFR1					486:489	NFR1	486:489	NFR1	486:489	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	37	theme	Nod	465:467	arg1	receptors					476:484	the Lotus japonicus Nod factor receptors	445:484	the Lotus japonicus Nod factor receptors NFR1 and NFR5	445:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	1	38	dep	composition	104:114	arg1	the					100:102	the	100:102	the	100:102	Understanding the composition and activation of multicomponent receptor complexes is a challenge in biology.
36656954	0	39	theme	root	63:66	arg1	symbiosis					75:83	root nodule symbiosis	63:83	root nodule symbiosis	63:83	Nanobody-driven signaling reveals the core receptor complex in root nodule symbiosis.
36656954	3	40	dep	receptors	476:484	arg1	NFR5					495:498	NFR5	495:498	NFR5	495:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	40	dep	receptors	476:484	arg1	NFR1					486:489	NFR1	486:489	NFR1	486:489	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36656954	3	40	dep	receptors	476:484	arg1	receptors					476:484	the Lotus japonicus Nod factor receptors	445:484	the Lotus japonicus Nod factor receptors NFR1 and NFR5	445:498	We show that the Lotus japonicus Nod factor receptors NFR1 and NFR5 constitute the core receptor complex initiating the cortical root nodule organogenesis program as well as the epidermal program controlling infection.
36613515	7	0	theme	glycogen	1599:1606	arg1	content					1608:1614	glycogen content	1599:1614	glycogen content of whole muscles	1599:1631	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	1	theme	glucose/glycogen	929:944	arg1	proteins					965:972	glucose/glycogen metabolism-related proteins	929:972	glucose/glycogen metabolism-related proteins	929:972	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	3	2	theme	altered	751:757	arg1	composition					773:783	altered myofiber-type composition	751:783	altered myofiber-type composition	751:783	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	6	3	theme	protein	1399:1405	arg1	levels					1407:1412	glucose/glycogen metabolism-related protein levels	1363:1412	glucose/glycogen metabolism-related protein levels	1363:1412	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	5	4	theme	male	1299:1302	arg1	mice					1304:1307	3-month-old WT and BLAJ male mice	1275:1307	3-month-old WT and BLAJ male mice	1275:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	4	5	theme	proteins	965:972	arg1	abundance					888:896	the abundance	884:896	the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	884:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	6	theme	BLAJ	1153:1156	arg1	mice					1163:1166	10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	1092:1166	10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	1092:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	6	7	theme	glucose/glycogen	1363:1378	arg1	levels					1407:1412	glucose/glycogen metabolism-related protein levels	1363:1412	glucose/glycogen metabolism-related protein levels	1363:1412	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	8	8	theme	deficiency	1817:1826	arg1	impact					1797:1802	a further impact	1787:1802	a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles	1787:1930	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	4	9	theme	Ca2+	910:913	arg1	handling					916:923	calcium (Ca2+) handling	901:923	calcium (Ca2+) handling	901:923	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	10	theme	handling	916:923	arg1	abundance					888:896	the abundance	884:896	the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	884:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	11	theme	soleus	1014:1019	arg1	muscles					1081:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles	977:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	977:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	1	12	from	post-growth	314:324	arg1	adults					335:340	young adults	329:340	young adults	329:340	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	3	13	theme	specific	612:619	arg1	differences					621:631	myofiber-type specific differences	598:631	myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition	598:783	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	4	14	theme	fast-twitch	1025:1035	arg1	EDL					1076:1078	EDL	1076:1078	EDL	1076:1078	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	14	theme	fast-twitch	1025:1035	arg1	longus					1068:1073	fast-twitch, glycolytic extensor digitorum longus	1025:1073	longus	1068:1073	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	6	15	theme	EDL	1438:1440	arg1	muscles					1442:1448	EDL muscles	1438:1448	EDL muscles	1438:1448	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	8	16	theme	glycogen	1892:1899	arg1	metabolism					1901:1910	glycogen metabolism	1892:1910	glycogen metabolism	1892:1910	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	5	17	theme	3-month-old	1275:1285	arg1	mice					1304:1307	3-month-old WT and BLAJ male mice	1275:1307	3-month-old WT and BLAJ male mice	1275:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	3	18	theme	contractile	643:653	arg1	function					655:662	muscle contractile function	636:662	muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice	636:708	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	4	19	from	abundance	888:896	arg1	muscles					1081:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles	977:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	977:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	3	20	theme	mice	705:708	arg1	function					655:662	muscle contractile function	636:662	muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice	636:708	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	7	21	theme	10-month-old	1702:1713	arg1	mice					1720:1723	10-month-old BLAJ mice	1702:1723	10-month-old BLAJ mice	1702:1723	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	5	22	theme	activation	1204:1213	arg1	properties					1215:1224	the Ca2+ activation properties	1195:1224	the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice	1195:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	3	23	theme	dysferlin-deficient	680:698	arg1	mice					705:708	10-month-old dysferlin-deficient BLAJ mice	667:708	10-month-old dysferlin-deficient BLAJ mice	667:708	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	0	24	theme	Calcium-Handling	84:99	arg1	Proteins					125:132	Calcium-Handling and Glycogen Metabolism Proteins	84:132	Calcium-Handling and Glycogen Metabolism Proteins	84:132	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	7	25	from	mice	1720:1723	arg1	muscle					1690:1695	soleus muscle	1683:1695	soleus muscle from 10-month-old BLAJ mice	1683:1723	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	26	from	impact	850:855	arg1	abundance					888:896	the abundance	884:896	the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	884:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	1	27	theme	muscular	193:200	arg1	dystrophies					202:212	muscular dystrophies	193:212	muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults	193:340	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	4	28	theme	glycolytic	1038:1047	arg1	EDL					1076:1078	EDL	1076:1078	EDL	1076:1078	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	28	theme	glycolytic	1038:1047	arg1	longus					1068:1073	fast-twitch, glycolytic extensor digitorum longus	1025:1073	longus	1068:1073	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	0	29	theme	Glycogen	105:112	arg1	Metabolism					114:123	Glycogen Metabolism	105:123	Glycogen Metabolism	105:123	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	8	30	theme	contractile	1969:1979	arg1	function					1981:1988	altered contractile function	1961:1988	altered contractile function in dysferlinopathy	1961:2007	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	7	31	theme	myofilaments	1660:1671	arg1	activation					1642:1651	Ca2+ activation	1637:1651	Ca2+ activation of the myofilaments	1637:1671	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	32	theme	digitorum	1058:1066	arg1	EDL					1076:1078	EDL	1076:1078	EDL	1076:1078	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	32	theme	digitorum	1058:1066	arg1	longus					1068:1073	fast-twitch, glycolytic extensor digitorum longus	1025:1073	longus	1068:1073	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	0	33	from	Differences	55:65	arg1	Abundances					70:79	Abundances	70:79	Abundances of Calcium-Handling and Glycogen Metabolism Proteins	70:132	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	0	34	theme	Proteins	125:132	arg1	Abundances					70:79	Abundances	70:79	Abundances of Calcium-Handling and Glycogen Metabolism Proteins	70:132	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	0	35	theme	Dysferlin	0:8	arg1	Deficiency					10:19	Dysferlin Deficiency	0:19	Dysferlin Deficiency	0:19	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	4	36	theme	C57BL/6J	1120:1127	arg1	mice					1163:1166	10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	1092:1166	10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	1092:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	2	37	theme	lipid	471:475	arg1	droplets					477:484	lipid droplets	471:484	lipid droplets	471:484	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	5	38	theme	WT	1287:1288	arg1	mice					1304:1307	3-month-old WT and BLAJ male mice	1275:1307	3-month-old WT and BLAJ male mice	1275:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	2	39	theme	later	519:523	arg1	stages					525:530	later stages	519:530	later stages	519:530	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	0	40	theme	Myofiber-Type	32:44	arg1	Differences					55:65	Myofiber-Type Specific Differences	32:65	Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins	32:132	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	5	41	theme	slow-	1238:1242	arg1	properties					1215:1224	the Ca2+ activation properties	1195:1224	the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice	1195:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	4	42	theme	wild-type	1105:1113	arg1	C57BL/6J					1120:1127	10-month-old wild-type (WT) C57BL/6J	1092:1127	10-month-old wild-type (WT) C57BL/6J	1092:1127	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	43	theme	calcium	901:907	arg1	handling					916:923	calcium (Ca2+) handling	901:923	calcium (Ca2+) handling	901:923	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	5	44	from	properties	1215:1224	arg1	mice					1304:1307	3-month-old WT and BLAJ male mice	1275:1307	3-month-old WT and BLAJ male mice	1275:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	5	45	theme	fast-twitch	1248:1258	arg1	myofibers					1260:1268	fast-twitch myofibers	1248:1268	fast-twitch myofibers	1248:1268	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	7	46	theme	whole	1619:1623	arg1	muscles					1625:1631	whole muscles	1619:1631	whole muscles	1619:1631	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	47	theme	dysferlin	860:868	arg1	deficiency					870:879	dysferlin deficiency	860:879	dysferlin deficiency	860:879	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	2	48	theme	progressive	375:385	arg1	muscle					396:401	progressive skeletal muscle	375:401	progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue	375:572	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	1	49	theme	membrane-associated	252:270	arg1	dysferlin					280:288	the membrane-associated protein dysferlin	248:288	the membrane-associated protein dysferlin	248:288	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	2	50	from	accumulation	455:466	arg1	myofibers					501:509	slow-twitch myofibers	489:509	slow-twitch myofibers	489:509	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	1	51	theme	dysferlin	280:288	arg1	deficiency					234:243	a genetic deficiency	224:243	a genetic deficiency	224:243	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	4	52	theme	dysferlin-deficient	1133:1151	arg1	BLAJ					1153:1156	dysferlin-deficient BLAJ	1133:1156	dysferlin-deficient BLAJ	1133:1156	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	53	dep	wild-type	1105:1113	arg1	WT					1116:1117	WT	1116:1117	WT	1116:1117	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	5	54	theme	BLAJ	1294:1297	arg1	mice					1304:1307	3-month-old WT and BLAJ male mice	1275:1307	3-month-old WT and BLAJ male mice	1275:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	6	55	dep	handling	1350:1357	arg1	some					1340:1343	some	1340:1343	some	1340:1343	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	4	56	theme	male	1158:1161	arg1	mice					1163:1166	10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	1092:1166	10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	1092:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	3	57	theme	myofiber-type	759:771	arg1	composition					773:783	altered myofiber-type composition	751:783	altered myofiber-type composition	751:783	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	6	58	theme	metabolism-related	1380:1397	arg1	levels					1407:1412	glucose/glycogen metabolism-related protein levels	1363:1412	glucose/glycogen metabolism-related protein levels	1363:1412	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	7	59	theme	Dysferlin	1563:1571	arg1	deficiency					1573:1582	Dysferlin deficiency	1563:1582	Dysferlin deficiency	1563:1582	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	60	theme	metabolism-related	946:963	arg1	proteins					965:972	glucose/glycogen metabolism-related proteins	929:972	glucose/glycogen metabolism-related proteins	929:972	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	8	61	theme	dysferlin	1807:1815	arg1	deficiency					1817:1826	dysferlin deficiency	1807:1826	dysferlin deficiency	1807:1826	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	1	62	theme	dystrophies	202:212	arg1	group					184:188	a clinically heterogeneous group	157:188	a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults	157:340	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	1	62	theme	dystrophies	202:212	arg1	dystrophies					202:212	muscular dystrophies	193:212	muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults	193:340	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	1	62	theme	dystrophies	202:212	arg1	Dysferlinopathies					135:151	Dysferlinopathies	135:151	Dysferlinopathies	135:151	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	8	63	from	metabolism	1901:1910	arg1	muscles					1924:1930	skeletal muscles	1915:1930	skeletal muscles	1915:1930	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	6	64	theme	Ca2+	1345:1348	arg1	handling					1350:1357	Ca2+ handling	1345:1357	Ca2+ handling	1345:1357	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	7	65	theme	EDL	1748:1750	arg1	muscles					1752:1758	EDL muscles	1748:1758	EDL muscles	1748:1758	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	5	66	from	mice	1304:1307	arg1	properties					1215:1224	the Ca2+ activation properties	1195:1224	the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice	1195:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	5	66	from	mice	1304:1307	arg1	slow-					1238:1242	isolated slow-	1229:1242	isolated slow-	1229:1242	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	5	66	from	mice	1304:1307	arg1	myofibers					1260:1268	fast-twitch myofibers	1248:1268	fast-twitch myofibers	1248:1268	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	3	67	theme	myofiber-type	598:610	arg1	differences					621:631	myofiber-type specific differences	598:631	myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition	598:783	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	1	68	theme	genetic	226:232	arg1	deficiency					234:243	a genetic deficiency	224:243	a genetic deficiency	224:243	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	4	69	theme	oxidative	1004:1012	arg1	soleus					1014:1019	predominantly slow-twitch, oxidative soleus	977:1019	soleus	1014:1019	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	6	70	from	function	1531:1538	arg1	muscles					1554:1560	these BLAJ muscles	1543:1560	these BLAJ muscles	1543:1560	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	8	71	theme	excitation-contraction	1856:1877	arg1	coupling					1879:1886	excitation-contraction coupling	1856:1886	excitation-contraction coupling	1856:1886	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	3	72	theme	muscle	636:641	arg1	function					655:662	muscle contractile function	636:662	muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice	636:708	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	7	73	theme	BLAJ	1715:1718	arg1	mice					1720:1723	10-month-old BLAJ mice	1702:1723	10-month-old BLAJ mice	1702:1723	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	6	74	theme	BLAJ	1422:1425	arg1	soleus					1427:1432	BLAJ soleus	1422:1432	BLAJ soleus	1422:1432	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	8	75	from	coupling	1879:1886	arg1	muscles					1924:1930	skeletal muscles	1915:1930	skeletal muscles	1915:1930	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	3	76	theme	BLAJ	700:703	arg1	mice					705:708	10-month-old dysferlin-deficient BLAJ mice	667:708	10-month-old dysferlin-deficient BLAJ mice	667:708	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	4	77	theme	slow-twitch	991:1001	arg1	soleus					1014:1019	predominantly slow-twitch, oxidative soleus	977:1019	soleus	1014:1019	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	3	78	from	differences	621:631	arg1	function					655:662	muscle contractile function	636:662	muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice	636:708	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	6	79	from	differences	1516:1526	arg1	function					1531:1538	function	1531:1538	function in these BLAJ muscles	1531:1560	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	3	80	theme	10-month-old	667:678	arg1	mice					705:708	10-month-old dysferlin-deficient BLAJ mice	667:708	10-month-old dysferlin-deficient BLAJ mice	667:708	Previously we reported myofiber-type specific differences in muscle contractile function of 10-month-old dysferlin-deficient BLAJ mice that could not be fully accounted for by altered myofiber-type composition.
36613515	8	81	theme	skeletal	1915:1922	arg1	muscles					1924:1930	skeletal muscles	1915:1930	skeletal muscles	1915:1930	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	1	82	theme	heterogeneous	170:182	arg1	group					184:188	a clinically heterogeneous group	157:188	a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults	157:340	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	1	82	theme	heterogeneous	170:182	arg1	dystrophies					202:212	muscular dystrophies	193:212	muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults	193:340	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	1	82	theme	heterogeneous	170:182	arg1	Dysferlinopathies					135:151	Dysferlinopathies	135:151	Dysferlinopathies	135:151	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	7	83	theme	soleus	1683:1688	arg1	muscle					1690:1695	soleus muscle	1683:1695	soleus muscle from 10-month-old BLAJ mice	1683:1723	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	84	theme	longus	1068:1073	arg1	muscles					1081:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles	977:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	977:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	8	85	from	function	1981:1988	arg1	dysferlinopathy					1993:2007	dysferlinopathy	1993:2007	dysferlinopathy	1993:2007	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	0	86	theme	Metabolism	114:123	arg1	Proteins					125:132	Calcium-Handling and Glycogen Metabolism Proteins	84:132	Calcium-Handling and Glycogen Metabolism Proteins	84:132	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	5	87	theme	isolated	1229:1236	arg1	slow-					1238:1242	isolated slow-	1229:1242	isolated slow-	1229:1242	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	2	88	theme	adipose	559:565	arg1	tissue					567:572	adipose tissue	559:572	adipose tissue	559:572	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	8	89	theme	altered	1961:1967	arg1	function					1981:1988	altered contractile function	1961:1988	altered contractile function in dysferlinopathy	1961:2007	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	2	90	theme	muscles	548:554	arg1	replacement					533:543	replacement	533:543	replacement of muscles by adipose tissue	533:572	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	6	91	theme	BLAJ	1549:1552	arg1	muscles					1554:1560	these BLAJ muscles	1543:1560	these BLAJ muscles	1543:1560	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	5	92	theme	Ca2+	1199:1202	arg1	properties					1215:1224	the Ca2+ activation properties	1195:1224	the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice	1195:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	2	93	theme	slow-twitch	489:499	arg1	myofibers					501:509	slow-twitch myofibers	489:509	slow-twitch myofibers	489:509	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	8	94	from	impact	1797:1802	arg1	proteins					1831:1838	proteins	1831:1838	proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles	1831:1930	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36613515	1	95	theme	young	329:333	arg1	adults					335:340	young adults	329:340	young adults	329:340	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	2	96	theme	droplets	477:484	arg1	accumulation					455:466	accumulation	455:466	accumulation of lipid droplets in slow-twitch myofibers	455:509	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	0	97	theme	Specific	46:53	arg1	Differences					55:65	Myofiber-Type Specific Differences	32:65	Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins	32:132	Dysferlin Deficiency Results in Myofiber-Type Specific Differences in Abundances of Calcium-Handling and Glycogen Metabolism Proteins.
36613515	4	98	theme	extensor	1049:1056	arg1	EDL					1076:1078	EDL	1076:1078	EDL	1076:1078	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	4	98	theme	extensor	1049:1056	arg1	longus					1068:1073	fast-twitch, glycolytic extensor digitorum longus	1025:1073	longus	1068:1073	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	6	99	theme	reported	1507:1514	arg1	differences					1516:1526	the previously reported differences	1492:1526	the previously reported differences in function in these BLAJ muscles	1492:1560	Differences were observed for some Ca2+ handling and glucose/glycogen metabolism-related protein levels between BLAJ soleus and EDL muscles (compared with WT) that may contribute to the previously reported differences in function in these BLAJ muscles.
36613515	7	100	theme	Ca2+	1637:1640	arg1	activation					1642:1651	Ca2+ activation	1637:1651	Ca2+ activation of the myofilaments	1637:1671	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	101	theme	10-month-old	1092:1103	arg1	C57BL/6J					1120:1127	10-month-old wild-type (WT) C57BL/6J	1092:1127	10-month-old wild-type (WT) C57BL/6J	1092:1127	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	2	102	theme	skeletal	387:394	arg1	muscle					396:401	progressive skeletal muscle	375:401	progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue	375:572	The disease is characterized by progressive skeletal muscle wasting in the limb-girdle and limbs, inflammation, accumulation of lipid droplets in slow-twitch myofibers and, in later stages, replacement of muscles by adipose tissue.
36613515	5	103	theme	myofibers	1260:1268	arg1	properties					1215:1224	the Ca2+ activation properties	1195:1224	the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice	1195:1307	Additionally, we compared the Ca2+ activation properties of isolated slow- and fast-twitch myofibers from 3-month-old WT and BLAJ male mice.
36613515	7	104	theme	muscles	1625:1631	arg1	content					1608:1614	glycogen content	1599:1614	glycogen content of whole muscles	1599:1631	Dysferlin deficiency did not impact glycogen content of whole muscles nor Ca2+ activation of the myofilaments, although soleus muscle from 10-month-old BLAJ mice had more glycogen than EDL muscles.
36613515	4	105	theme	mice	1163:1166	arg1	muscles					1081:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles	977:1087	predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	977:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	1	106	theme	protein	272:278	arg1	dysferlin					280:288	the membrane-associated protein dysferlin	248:288	the membrane-associated protein dysferlin	248:288	Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by a genetic deficiency of the membrane-associated protein dysferlin, which usually manifest post-growth in young adults.
36613515	4	107	theme	deficiency	870:879	arg1	impact					850:855	the impact	846:855	the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice	846:1166	In order to further investigate these findings, we examined the impact of dysferlin deficiency on the abundance of calcium (Ca2+) handling and glucose/glycogen metabolism-related proteins in predominantly slow-twitch, oxidative soleus and fast-twitch, glycolytic extensor digitorum longus (EDL) muscles of 10-month-old wild-type (WT) C57BL/6J and dysferlin-deficient BLAJ male mice.
36613515	8	108	theme	further	1789:1795	arg1	impact					1797:1802	a further impact	1787:1802	a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles	1787:1930	These results demonstrate a further impact of dysferlin deficiency on proteins associated with excitation-contraction coupling and glycogen metabolism in skeletal muscles, potentially contributing to altered contractile function in dysferlinopathy.
36361854	0	0	theme	Arthritis	106:114	arg1	Models					96:101	In Vivo Models	88:101	In Vivo Models of Arthritis	88:114	Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis.
36361854	10	1	from	CIA	1340:1342	arg1	mice					1364:1367	mice	1364:1367	mice	1364:1367	Results show that BPI attenuates CIA and inflammation in mice, and this effect is enhanced by HA co-administration.
36361854	11	2	theme	new	1492:1494	arg1	treatments					1506:1515	new potential treatments	1492:1515	new potential treatments in arthritis	1492:1528	Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
36361854	7	3	dep	outcomes	879:886	arg1	scores					902:907	histological scores	889:907	histological scores ≥2-fold	889:915	In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47-93%).
36361854	11	4	theme	Combined	1423:1430	arg1	use					1432:1434	Combined use	1423:1434	Combined use of BPI and HA	1423:1448	Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
36361854	11	5	theme	HA	1447:1448	arg1	use					1432:1434	Combined use	1423:1434	Combined use of BPI and HA	1423:1448	Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
36361854	6	6	theme	Cytokine	765:772	arg1	levels					774:779	Cytokine levels	765:779	Cytokine levels	765:779	Cytokine levels were measured in serum and peritoneal fluids.
36361854	7	7	theme	CIA	830:832	arg1	mice					834:837	CIA mice	830:837	CIA mice	830:837	In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47-93%).
36361854	1	8	theme	collagen-induced	271:286	arg1	CIA					299:301	CIA	299:301	CIA	299:301	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	8	theme	collagen-induced	271:286	arg1	models					263:268	two animal models	252:268	two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation	252:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	8	theme	collagen-induced	271:286	arg1	arthritis					288:296	collagen-induced arthritis	271:296	collagen-induced arthritis (CIA)	271:302	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	3	9	theme	ankle	521:525	arg1	joints					527:532	ankle joints	521:532	ankle joints	521:532	Arthritis was assessed in ankle joints by clinical and histological evaluation.
36361854	8	10	theme	crystal-induced	973:987	arg1	inflammation					989:1000	crystal-induced inflammation	973:1000	crystal-induced inflammation	973:1000	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	9	11	with	co-treated	1164:1173	arg1	HA					1188:1189	HA	1188:1189	HA	1188:1189	In both models, when mice were co-treated with BPI and HA, the improvement of all parameters was greater than that observed after administration of the two substances alone.
36361854	9	11	with	co-treated	1164:1173	arg1	BPI					1180:1182	BPI	1180:1182	BPI	1180:1182	In both models, when mice were co-treated with BPI and HA, the improvement of all parameters was greater than that observed after administration of the two substances alone.
36361854	4	12	theme	plus	635:638	arg1	mice					630:633	mice	630:633	mice plus or minus BPI	630:651	Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA.
36361854	8	13	dep	infiltration	1025:1036	arg1	count					1045:1049	total count	1039:1049	total count: ≥60%; polymorphonuclear cells: ≥36%	1039:1086	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	0	14	from	Response	76:83	arg1	Models					96:101	In Vivo Models	88:101	In Vivo Models of Arthritis	88:114	Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis.
36361854	8	15	dep	count	1045:1049	arg1	%					1055:1055	≥60%	1052:1055	total count: ≥60%; polymorphonuclear cells: ≥36%	1039:1086	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	8	15	dep	count	1045:1049	arg1	cells					1076:1080	polymorphonuclear cells	1058:1080	total count: ≥60%; polymorphonuclear cells: ≥36%	1039:1086	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	8	15	dep	count	1045:1049	arg1	%					1086:1086	≥36%	1083:1086	total count: ≥60%; polymorphonuclear cells: ≥36%	1039:1086	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	8	16	theme	cytokine	1103:1110	arg1	production					1112:1121	cytokine production	1103:1121	cytokine production (35-74%)	1103:1130	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	8	16	theme	cytokine	1103:1110	arg1	%					1129:1129	35-74%	1124:1129	35-74%	1124:1129	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	8	17	theme	leukocyte	1015:1023	arg1	infiltration					1025:1036	leukocyte infiltration	1015:1036	leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%)	1015:1087	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	7	18	theme	histological	889:900	arg1	scores					902:907	histological scores	889:907	histological scores ≥2-fold	889:915	In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47-93%).
36361854	4	19	theme	HA	687:688	arg1	composition					664:674	a composition	662:674	a composition of BPI and HA	662:688	Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA.
36361854	4	20	theme	minus	643:647	arg1	mice					630:633	mice	630:633	mice plus or minus BPI	630:651	Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA.
36361854	0	21	theme	Bactericidal/Permeability-Increasing	0:35	arg1	Protein					37:43	Bactericidal/Permeability-Increasing Protein	0:43	Bactericidal/Permeability-Increasing Protein	0:43	Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis.
36361854	9	22	theme	parameters	1215:1224	arg1	greater					1230:1236	greater	1230:1236	greater	1230:1236	In both models, when mice were co-treated with BPI and HA, the improvement of all parameters was greater than that observed after administration of the two substances alone.
36361854	9	22	theme	parameters	1215:1224	arg1	improvement					1196:1206	the improvement	1192:1206	the improvement of all parameters	1192:1224	In both models, when mice were co-treated with BPI and HA, the improvement of all parameters was greater than that observed after administration of the two substances alone.
36361854	1	23	from	effects	137:143	arg1	inflammation					324:335	crystal-induced inflammation	308:335	crystal-induced inflammation	308:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	23	from	effects	137:143	arg1	models					263:268	two animal models	252:268	two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation	252:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	23	from	effects	137:143	arg1	arthritis					288:296	collagen-induced arthritis	271:296	collagen-induced arthritis (CIA)	271:302	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	9	24	theme	substances	1289:1298	arg1	administration					1263:1276	administration	1263:1276	administration of the two substances alone	1263:1304	In both models, when mice were co-treated with BPI and HA, the improvement of all parameters was greater than that observed after administration of the two substances alone.
36361854	7	25	theme	histological	866:877	arg1	outcomes					879:886	clinical and histological outcomes	853:886	clinical and histological outcomes (histological scores ≥2-fold)	853:916	In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47-93%).
36361854	11	26	theme	BPI	1439:1441	arg1	use					1432:1434	Combined use	1423:1434	Combined use of BPI and HA	1423:1448	Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
36361854	2	27	theme	minus	412:416	arg1	HA					418:419	BPI plus or minus HA	400:419	BPI plus or minus HA	400:419	In CIA, mice were intraperitoneally injected with PBS, HA, or BPI plus or minus HA, twice a week for 2 months, and then euthanized to collect paw and blood.
36361854	10	28	theme	HA	1401:1402	arg1	co-administration					1404:1420	HA co-administration	1401:1420	HA co-administration	1401:1420	Results show that BPI attenuates CIA and inflammation in mice, and this effect is enhanced by HA co-administration.
36361854	5	29	theme	differential	718:729	arg1	counts					741:746	differential leukocyte counts	718:746	differential leukocyte counts	718:746	After sacrifice, total and differential leukocyte counts were determined.
36361854	11	30	theme	potential	1496:1504	arg1	treatments					1506:1515	new potential treatments	1492:1515	new potential treatments in arthritis	1492:1528	Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
36361854	0	31	dep	In	88:89	arg1	Vivo					91:94	Vivo	91:94	Vivo	91:94	Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis.
36361854	11	32	from	treatments	1506:1515	arg1	arthritis					1520:1528	arthritis	1520:1528	arthritis	1520:1528	Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
36361854	2	33	theme	plus	404:407	arg1	HA					418:419	BPI plus or minus HA	400:419	BPI plus or minus HA	400:419	In CIA, mice were intraperitoneally injected with PBS, HA, or BPI plus or minus HA, twice a week for 2 months, and then euthanized to collect paw and blood.
36361854	0	34	theme	Inflammatory	63:74	arg1	Response					76:83	the Inflammatory Response	59:83	the Inflammatory Response in In Vivo Models of Arthritis	59:114	Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis.
36361854	1	35	theme	crystal-induced	308:322	arg1	inflammation					324:335	crystal-induced inflammation	308:335	crystal-induced inflammation	308:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	35	theme	crystal-induced	308:322	arg1	models					263:268	two animal models	252:268	two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation	252:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	5	36	theme	leukocyte	731:739	arg1	counts					741:746	differential leukocyte counts	718:746	differential leukocyte counts	718:746	After sacrifice, total and differential leukocyte counts were determined.
36361854	6	37	theme	peritoneal	808:817	arg1	fluids					819:824	peritoneal fluids	808:824	peritoneal fluids	808:824	Cytokine levels were measured in serum and peritoneal fluids.
36361854	3	38	theme	histological	550:561	arg1	evaluation					563:572	clinical and histological evaluation	537:572	clinical and histological evaluation	537:572	Arthritis was assessed in ankle joints by clinical and histological evaluation.
36361854	11	39	theme	interesting	1464:1474	arg1	perspective					1476:1486	an interesting perspective	1461:1486	an interesting perspective for new potential treatments in arthritis	1461:1528	Combined use of BPI and HA represents an interesting perspective for new potential treatments in arthritis.
36361854	0	40	theme	In	88:89	arg1	Models					96:101	In Vivo Models	88:101	In Vivo Models of Arthritis	88:114	Bactericidal/Permeability-Increasing Protein Downregulates the Inflammatory Response in In Vivo Models of Arthritis.
36361854	10	41	from	inflammation	1348:1359	arg1	mice					1364:1367	mice	1364:1367	mice	1364:1367	Results show that BPI attenuates CIA and inflammation in mice, and this effect is enhanced by HA co-administration.
36361854	1	42	with	combination	211:221	arg1	acid					239:242	hyaluronic acid	228:242	hyaluronic acid (HA)	228:247	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	42	with	combination	211:221	arg1	HA					245:246	HA	245:246	HA	245:246	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	8	43	theme	total	1039:1043	arg1	count					1045:1049	total count	1039:1049	total count: ≥60%; polymorphonuclear cells: ≥36%	1039:1086	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	3	44	theme	clinical	537:544	arg1	evaluation					563:572	clinical and histological evaluation	537:572	clinical and histological evaluation	537:572	Arthritis was assessed in ankle joints by clinical and histological evaluation.
36361854	7	45	theme	clinical	853:860	arg1	outcomes					879:886	clinical and histological outcomes	853:886	clinical and histological outcomes (histological scores ≥2-fold)	853:916	In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47-93%).
36361854	4	46	theme	BPI	679:681	arg1	composition					664:674	a composition	662:674	a composition of BPI and HA	662:688	Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA.
36361854	1	47	dep	models	263:268	arg1	inflammation					324:335	crystal-induced inflammation	308:335	crystal-induced inflammation	308:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	47	dep	models	263:268	arg1	CIA					299:301	CIA	299:301	CIA	299:301	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	47	dep	models	263:268	arg1	models					263:268	two animal models	252:268	two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation	252:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	47	dep	models	263:268	arg1	arthritis					288:296	collagen-induced arthritis	271:296	collagen-induced arthritis (CIA)	271:302	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	7	48	theme	inflammatory	937:948	arg1	mediators					950:958	inflammatory mediators	937:958	inflammatory mediators (47-93%)	937:967	In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47-93%).
36361854	7	48	theme	inflammatory	937:948	arg1	%					966:966	47-93%	961:966	47-93%	961:966	In CIA mice, BPI improved clinical and histological outcomes (histological scores ≥2-fold), and downregulated inflammatory mediators (47-93%).
36361854	1	49	theme	protein	185:191	arg1	effects					137:143	the effects	133:143	the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation	133:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	50	theme	bactericidal/permeability-increasing	148:183	arg1	BPI					194:196	BPI	194:196	BPI	194:196	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	50	theme	bactericidal/permeability-increasing	148:183	arg1	protein					185:191	bactericidal/permeability-increasing protein	148:191	bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA)	148:247	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	4	51	dep	plus	635:638	arg1	BPI					649:651	BPI	649:651	BPI	649:651	Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA.
36361854	1	52	theme	hyaluronic	228:237	arg1	acid					239:242	hyaluronic acid	228:242	hyaluronic acid (HA)	228:247	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	52	theme	hyaluronic	228:237	arg1	HA					245:246	HA	245:246	HA	245:246	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	8	53	theme	polymorphonuclear	1058:1074	arg1	cells					1076:1080	polymorphonuclear cells	1058:1080	total count: ≥60%; polymorphonuclear cells: ≥36%	1039:1086	In crystal-induced inflammation, BPI reduced leukocyte infiltration (total count: ≥60%; polymorphonuclear cells: ≥36%) and inhibited cytokine production (35-74%).
36361854	4	54	theme	Pathogenic	575:584	arg1	crystals					586:593	Pathogenic crystals	575:593	Pathogenic crystals	575:593	Pathogenic crystals were intraperitoneally injected in mice plus or minus BPI, or with a composition of BPI and HA.
36361854	1	55	theme	animal	256:261	arg1	inflammation					324:335	crystal-induced inflammation	308:335	crystal-induced inflammation	308:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	55	theme	animal	256:261	arg1	models					263:268	two animal models	252:268	two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation	252:335	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
36361854	1	55	theme	animal	256:261	arg1	arthritis					288:296	collagen-induced arthritis	271:296	collagen-induced arthritis (CIA)	271:302	We investigated the effects of bactericidal/permeability-increasing protein (BPI) alone or in combination with hyaluronic acid (HA) in two animal models: collagen-induced arthritis (CIA) and crystal-induced inflammation.
37312624	5	0	theme	leaf	806:809	arg1	exchange					815:822	leaf gas exchange	806:822	leaf gas exchange	806:822	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	9	1	from	impact	1357:1362	arg1	CRing					1396:1400	δ13 CRing	1392:1400	δ13 CRing	1392:1400	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	1	from	impact	1357:1362	arg1	photosynthates					1438:1451	photosynthates	1438:1451	photosynthates compared ring organic matter and tree-ring cellulose, respectively	1438:1518	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	8	2	theme	same	1217:1220	arg1	extracts					1222:1229	the same extracts	1213:1229	the same extracts	1213:1229	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	4	3	theme	Isotope	474:480	arg1	processes					496:504	Isotope fractionation processes	474:504	Isotope fractionation processes	474:504	Isotope fractionation processes, which are not yet fully understood, modify δ13 CP during sucrose transport.
37312624	7	4	theme	down-stem	1046:1054	arg1	transport					1056:1064	down-stem transport	1046:1064	down-stem transport	1046:1064	However, δ13 CP became increasingly 13 C-enriched during down-stem transport, probably due to post-photosynthetic fractionations such as sink organ catabolism.
37312624	3	5	theme	CP	469:470	arg1	record					455:460	a record	453:460	a record of δ13 CP	453:470	However, δ13 CRing is not merely a record of δ13 CP .
37312624	3	5	theme	CP	469:470	arg1	CRing					433:437	δ13 CRing	429:437	δ13 CRing	429:437	However, δ13 CRing is not merely a record of δ13 CP .
37312624	0	6	theme	compound-specific	89:105	arg1	analysis					134:141	compound-specific and laser ablation isotope analysis	89:141	analysis	134:141	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	5	7	theme	environmental	602:614	arg1	signal					638:643	the environmental intra-seasonal δ13 CP signal	598:643	the environmental intra-seasonal δ13 CP signal	598:643	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	8	8	theme	isotope	1257:1263	arg1	dynamics					1265:1272	the same isotope dynamics	1248:1272	the same isotope dynamics	1248:1272	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	9	9	theme	organic	1467:1473	arg1	matter					1475:1480	ring organic matter	1462:1480	ring organic matter	1462:1480	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	4	10	theme	δ13	550:552	arg1	CP					554:555	δ13 CP	550:555	δ13 CP	550:555	Isotope fractionation processes, which are not yet fully understood, modify δ13 CP during sucrose transport.
37312624	5	11	theme	intra-seasonal	616:629	arg1	signal					638:643	the environmental intra-seasonal δ13 CP signal	598:643	the environmental intra-seasonal δ13 CP signal	598:643	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	8	12	theme	same	1252:1255	arg1	dynamics					1265:1272	the same isotope dynamics	1248:1272	the same isotope dynamics	1248:1272	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	9	13	theme	signals	1381:1387	arg1	depletion					1425:1433	the 0.5 and 1.7‰ depletion	1408:1433	the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively	1408:1518	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	13	theme	signals	1381:1387	arg1	impact					1357:1362	The impact	1353:1362	The impact of environmental signals on δ13 CRing	1353:1400	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	13	theme	signals	1381:1387	arg1	pieces					1532:1537	useful pieces	1525:1537	useful pieces of information for studies exploiting δ13 CRing	1525:1585	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	5	14	theme	CRing	784:788	arg1	ablation					796:803	δ13 CRing laser ablation	780:803	δ13 CRing laser ablation	780:803	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	0	15	theme	laser	111:115	arg1	analysis					134:141	compound-specific and laser ablation isotope analysis	89:141	analysis	134:141	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	5	16	theme	enzyme	828:833	arg1	measurements					844:855	enzyme activity measurements	828:855	enzyme activity measurements	828:855	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	7	17	theme	post-photosynthetic	1083:1101	arg1	catabolism					1137:1146	sink organ catabolism	1126:1146	sink organ catabolism	1126:1146	However, δ13 CP became increasingly 13 C-enriched during down-stem transport, probably due to post-photosynthetic fractionations such as sink organ catabolism.
37312624	7	17	theme	post-photosynthetic	1083:1101	arg1	fractionations					1103:1116	post-photosynthetic fractionations	1083:1116	post-photosynthetic fractionations such as sink organ catabolism	1083:1146	However, δ13 CP became increasingly 13 C-enriched during down-stem transport, probably due to post-photosynthetic fractionations such as sink organ catabolism.
37312624	5	18	theme	carbohydrates	765:777	arg1	exchange					815:822	leaf gas exchange	806:822	leaf gas exchange	806:822	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	5	18	theme	carbohydrates	765:777	arg1	measurements					844:855	enzyme activity measurements	828:855	enzyme activity measurements	828:855	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	5	18	theme	carbohydrates	765:777	arg1	ablation					796:803	δ13 CRing laser ablation	780:803	δ13 CRing laser ablation	780:803	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	5	18	theme	carbohydrates	765:777	arg1	analysis					742:749	δ13 C analysis	736:749	δ13 C analysis of individual carbohydrates	736:777	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	2	19	theme	CRing	270:274	arg1	reconstructions					276:290	δ13 CRing reconstructions	266:290	δ13 CRing reconstructions	266:290	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	1	20	theme	tree-ring	174:182	arg1	composition					159:169	Carbon isotope composition	144:169	Carbon isotope composition of tree-ring (δ13 CRing )	144:195	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	1	20	theme	tree-ring	174:182	arg1	proxy					216:220	a commonly used proxy	200:220	a commonly used proxy for environmental change and ecophysiology	200:263	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	7	21	theme	sink	1126:1129	arg1	catabolism					1137:1146	sink organ catabolism	1126:1146	sink organ catabolism	1126:1146	However, δ13 CP became increasingly 13 C-enriched during down-stem transport, probably due to post-photosynthetic fractionations such as sink organ catabolism.
37312624	2	22	theme	δ13	266:268	arg1	reconstructions					276:290	δ13 CRing reconstructions	266:290	δ13 CRing reconstructions	266:290	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	5	23	theme	laser	790:794	arg1	ablation					796:803	δ13 CRing laser ablation	780:803	δ13 CRing laser ablation	780:803	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	5	24	theme	δ13	736:738	arg1	analysis					742:749	δ13 C analysis	736:749	δ13 C analysis of individual carbohydrates	736:777	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	5	25	dep	Pinus	712:716	arg1	sylvestris					718:727	sylvestris	718:727	sylvestris	718:727	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	2	26	theme	solid	307:311	arg1	knowledge					313:321	a solid knowledge	305:321	a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose	305:417	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	8	27	theme	water-soluble	1171:1183	arg1	carbohydrates					1185:1197	water-soluble carbohydrates	1171:1197	water-soluble carbohydrates	1171:1197	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	5	28	theme	C	740:740	arg1	analysis					742:749	δ13 C analysis	736:749	δ13 C analysis of individual carbohydrates	736:777	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	9	29	theme	tree-ring	1486:1494	arg1	cellulose					1496:1504	tree-ring cellulose	1486:1504	tree-ring cellulose	1486:1504	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	8	30	theme	carbohydrates	1185:1197	arg1	C					1166:1166	δ13 C	1162:1166	δ13 C of water-soluble carbohydrates, analysed for the same extracts,	1162:1230	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	2	31	theme	photosynthates	377:390	arg1	formation					356:364	formation	356:364	formation of primary photosynthates (δ13 CP ), such as sucrose	356:417	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	0	32	theme	δ13	26:28	arg1	C					30:30	intra-seasonal δ13 C	11:30	intra-seasonal δ13 C	11:30	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	9	33	theme	information	1542:1552	arg1	depletion					1425:1433	the 0.5 and 1.7‰ depletion	1408:1433	the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively	1408:1518	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	33	theme	information	1542:1552	arg1	impact					1357:1362	The impact	1353:1362	The impact of environmental signals on δ13 CRing	1353:1400	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	33	theme	information	1542:1552	arg1	pieces					1532:1537	useful pieces	1525:1537	useful pieces of information for studies exploiting δ13 CRing	1525:1585	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	0	34	theme	isotope	126:132	arg1	analysis					134:141	compound-specific and laser ablation isotope analysis	89:141	analysis	134:141	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	9	35	from	depletion	1425:1433	arg1	CRing					1396:1400	δ13 CRing	1392:1400	δ13 CRing	1392:1400	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	35	from	depletion	1425:1433	arg1	photosynthates					1438:1451	photosynthates	1438:1451	photosynthates compared ring organic matter and tree-ring cellulose, respectively	1438:1518	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	5	36	theme	individual	754:763	arg1	carbohydrates					765:777	individual carbohydrates	754:777	individual carbohydrates	754:777	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	0	37	theme	intra-seasonal	11:24	arg1	C					30:30	intra-seasonal δ13 C	11:30	intra-seasonal δ13 C	11:30	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	0	38	theme	ablation	117:124	arg1	analysis					134:141	compound-specific and laser ablation isotope analysis	89:141	analysis	134:141	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	1	39	theme	Carbon	144:149	arg1	composition					159:169	Carbon isotope composition	144:169	Carbon isotope composition of tree-ring (δ13 CRing )	144:195	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	1	39	theme	Carbon	144:149	arg1	proxy					216:220	a commonly used proxy	200:220	a commonly used proxy for environmental change and ecophysiology	200:263	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	7	40	theme	organ	1131:1135	arg1	catabolism					1137:1146	sink organ catabolism	1126:1146	sink organ catabolism	1126:1146	However, δ13 CP became increasingly 13 C-enriched during down-stem transport, probably due to post-photosynthetic fractionations such as sink organ catabolism.
37312624	5	41	theme	activity	835:842	arg1	measurements					844:855	enzyme activity measurements	828:855	enzyme activity measurements	828:855	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	6	42	theme	use	970:972	arg1	impact					952:957	negligible impact	941:957	negligible impact of reserve use on δ13 CRing	941:985	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	9	43	theme	environmental	1367:1379	arg1	signals					1381:1387	environmental signals	1367:1387	environmental signals	1367:1387	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	3	44	theme	δ13	429:431	arg1	record					455:460	a record	453:460	a record of δ13 CP	453:470	However, δ13 CRing is not merely a record of δ13 CP .
37312624	3	44	theme	δ13	429:431	arg1	CRing					433:437	δ13 CRing	429:437	δ13 CRing	429:437	However, δ13 CRing is not merely a record of δ13 CP .
37312624	0	45	theme	C	30:30	arg1	Drivers					0:6	Drivers	0:6	Drivers of intra-seasonal δ13 C	0:30	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	6	46	theme	CP	881:882	arg1	dynamics					884:891	The intra-seasonal δ13 CP dynamics	858:891	The intra-seasonal δ13 CP dynamics	858:891	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	5	47	theme	δ13	780:782	arg1	ablation					796:803	δ13 CRing laser ablation	780:803	δ13 CRing laser ablation	780:803	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	4	48	theme	fractionation	482:494	arg1	processes					496:504	Isotope fractionation processes	474:504	Isotope fractionation processes	474:504	Isotope fractionation processes, which are not yet fully understood, modify δ13 CP during sucrose transport.
37312624	5	49	theme	δ13	631:633	arg1	signal					638:643	the environmental intra-seasonal δ13 CP signal	598:643	the environmental intra-seasonal δ13 CP signal	598:643	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	7	50	theme	δ13	998:1000	arg1	CP					1002:1003	δ13 CP	998:1003	δ13 CP	998:1003	However, δ13 CP became increasingly 13 C-enriched during down-stem transport, probably due to post-photosynthetic fractionations such as sink organ catabolism.
37312624	8	51	theme	intra-seasonal	1318:1331	arg1	variability					1340:1350	intra-seasonal δ13 CP variability	1318:1350	intra-seasonal δ13 CP variability	1318:1350	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	2	52	theme	fractionations	334:347	arg1	knowledge					313:321	a solid knowledge	305:321	a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose	305:417	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	9	53	theme	0.5 and	1412:1418	arg1	depletion					1425:1433	the 0.5 and 1.7‰ depletion	1408:1433	the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively	1408:1518	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	53	theme	0.5 and	1412:1418	arg1	impact					1357:1362	The impact	1353:1362	The impact of environmental signals on δ13 CRing	1353:1400	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	53	theme	0.5 and	1412:1418	arg1	pieces					1532:1537	useful pieces	1525:1537	useful pieces of information for studies exploiting δ13 CRing	1525:1585	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	6	54	theme	intra-seasonal	862:875	arg1	dynamics					884:891	The intra-seasonal δ13 CP dynamics	858:891	The intra-seasonal δ13 CP dynamics	858:891	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	5	55	theme	CP	635:636	arg1	signal					638:643	the environmental intra-seasonal δ13 CP signal	598:643	the environmental intra-seasonal δ13 CP signal	598:643	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	9	56	theme	useful	1525:1530	arg1	depletion					1425:1433	the 0.5 and 1.7‰ depletion	1408:1433	the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively	1408:1518	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	56	theme	useful	1525:1530	arg1	impact					1357:1362	The impact	1353:1362	The impact of environmental signals on δ13 CRing	1353:1400	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	56	theme	useful	1525:1530	arg1	pieces					1532:1537	useful pieces	1525:1537	useful pieces of information for studies exploiting δ13 CRing	1525:1585	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	2	57	theme	isotope	326:332	arg1	fractionations					334:347	isotope fractionations	326:347	isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose	326:417	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	9	58	theme	ring	1462:1465	arg1	matter					1475:1480	ring organic matter	1462:1480	ring organic matter	1462:1480	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	8	59	theme	CP	1337:1338	arg1	variability					1340:1350	intra-seasonal δ13 CP variability	1318:1350	intra-seasonal δ13 CP variability	1318:1350	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	1	60	theme	used	211:214	arg1	composition					159:169	Carbon isotope composition	144:169	Carbon isotope composition of tree-ring (δ13 CRing )	144:195	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	1	60	theme	used	211:214	arg1	proxy					216:220	a commonly used proxy	200:220	a commonly used proxy for environmental change and ecophysiology	200:263	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	8	61	theme	δ13	1333:1335	arg1	variability					1340:1350	intra-seasonal δ13 CP variability	1318:1350	intra-seasonal δ13 CP variability	1318:1350	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	5	62	theme	gas	811:813	arg1	exchange					815:822	leaf gas exchange	806:822	leaf gas exchange	806:822	We traced, how the environmental intra-seasonal δ13 CP signal changes from leaves to phloem, tree-ring and roots, for 7 year old Pinus sylvestris, using δ13 C analysis of individual carbohydrates, δ13 CRing laser ablation, leaf gas exchange and enzyme activity measurements.
37312624	9	63	theme	δ13	1577:1579	arg1	CRing					1581:1585	δ13 CRing	1577:1585	δ13 CRing	1577:1585	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	0	64	theme	Pinus sylvestris	56:71	arg1	tree-rings					42:51	tree-rings	42:51	tree-rings of Pinus sylvestris	42:71	Drivers of intra-seasonal δ13 C signal in tree-rings of Pinus sylvestris as indicated by compound-specific and laser ablation isotope analysis.
37312624	6	65	theme	reserve	962:968	arg1	use					970:972	reserve use	962:972	reserve use	962:972	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	6	66	from	impact	952:957	arg1	CRing					981:985	δ13 CRing	977:985	δ13 CRing	977:985	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	9	67	theme	1.7‰	1420:1423	arg1	depletion					1425:1433	the 0.5 and 1.7‰ depletion	1408:1433	the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively	1408:1518	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	67	theme	1.7‰	1420:1423	arg1	impact					1357:1362	The impact	1353:1362	The impact of environmental signals on δ13 CRing	1353:1400	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	9	67	theme	1.7‰	1420:1423	arg1	pieces					1532:1537	useful pieces	1525:1537	useful pieces of information for studies exploiting δ13 CRing	1525:1585	The impact of environmental signals on δ13 CRing , and the 0.5 and 1.7‰ depletion in photosynthates compared ring organic matter and tree-ring cellulose, respectively, are useful pieces of information for studies exploiting δ13 CRing .
37312624	2	68	theme	primary	369:375	arg1	photosynthates					377:390	primary photosynthates	369:390	primary photosynthates (δ13 CP )	369:400	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	2	68	theme	primary	369:375	arg1	CP					397:398	δ13 CP	393:398	δ13 CP	393:398	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	2	68	theme	primary	369:375	arg1	sucrose					411:417	sucrose	411:417	sucrose	411:417	δ13 CRing reconstructions are based on a solid knowledge of isotope fractionations during formation of primary photosynthates (δ13 CP ), such as sucrose.
37312624	8	69	theme	δ13	1162:1164	arg1	C					1166:1166	δ13 C	1162:1166	δ13 C of water-soluble carbohydrates, analysed for the same extracts,	1162:1230	In contrast, δ13 C of water-soluble carbohydrates, analysed for the same extracts, did not reflect the same isotope dynamics and fractionations as δ13 CP , but recorded intra-seasonal δ13 CP variability.
37312624	6	70	theme	negligible	941:950	arg1	impact					952:957	negligible impact	941:957	negligible impact of reserve use on δ13 CRing	941:985	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	6	71	theme	δ13	918:920	arg1	CRing					922:926	δ13 CRing	918:926	δ13 CRing	918:926	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	4	72	theme	sucrose	564:570	arg1	transport					572:580	sucrose transport	564:580	sucrose transport	564:580	Isotope fractionation processes, which are not yet fully understood, modify δ13 CP during sucrose transport.
37312624	1	73	theme	isotope	151:157	arg1	composition					159:169	Carbon isotope composition	144:169	Carbon isotope composition of tree-ring (δ13 CRing )	144:195	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	1	73	theme	isotope	151:157	arg1	proxy					216:220	a commonly used proxy	200:220	a commonly used proxy for environmental change and ecophysiology	200:263	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
37312624	6	74	theme	δ13	877:879	arg1	dynamics					884:891	The intra-seasonal δ13 CP dynamics	858:891	The intra-seasonal δ13 CP dynamics	858:891	The intra-seasonal δ13 CP dynamics was clearly reflected by δ13 CRing , suggesting negligible impact of reserve use on δ13 CRing .
37312624	1	75	theme	environmental	226:238	arg1	change					240:245	environmental change	226:245	environmental change	226:245	Carbon isotope composition of tree-ring (δ13 CRing ) is a commonly used proxy for environmental change and ecophysiology.
35997915	0	0	theme	thermostable	87:98	arg1	glucoamylase					100:111	thermostable glucoamylase	87:111	thermostable glucoamylase from Thermoanaerobacter ethanolicus	87:147	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	5	1	theme	wide	715:718	arg1	3.0-10.0					730:737	3.0-10.0	730:737	3.0-10.0	730:737	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	1	theme	wide	715:718	arg1	range					723:727	a wide pH range	713:727	a wide pH range (3.0-10.0)	713:738	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	2	theme	highest	612:618	arg1	activities					629:638	the highest specific activities	608:638	the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg)	608:688	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	8	3	from	use	1140:1142	arg1	processes					1168:1176	different industrial processes	1147:1176	different industrial processes such as biofuel production and food processing	1147:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	8	3	from	use	1140:1142	arg1	production					1194:1203	biofuel production	1186:1203	biofuel production	1186:1203	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	8	3	from	use	1140:1142	arg1	processing					1214:1223	food processing	1209:1223	food processing	1209:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	3	4	dep	METHODS	363:369	arg1	optimized					405:413	optimized	405:413	was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium)	401:526	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	5	5	theme	pH	720:721	arg1	3.0-10.0					730:737	3.0-10.0	730:737	3.0-10.0	730:737	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	5	theme	pH	720:721	arg1	range					723:727	a wide pH range	713:727	a wide pH range (3.0-10.0)	713:738	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	6	from	range	723:727	arg1	stable					703:708	stable	703:708	stable	703:708	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	7	theme	specific	620:627	arg1	activities					629:638	the highest specific activities	608:638	the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg)	608:688	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	8	8	theme	food	1209:1212	arg1	processing					1214:1223	food processing	1209:1223	food processing	1209:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	4	9	theme	high	551:554	arg1	temperature					564:574	a high optimum temperature	549:574	a high optimum temperature of 75 °C	549:583	RESULTS TeGA showed a high optimum temperature of 75 °C.
35997915	6	10	theme	different	831:839	arg1	starches					849:856	different soluble starches	831:856	different soluble starches such as those from potato, corn or rice	831:896	Although the enzyme was preferentially active with maltose, it was also able to hydrolyze different soluble starches such as those from potato, corn or rice.
35997915	6	10	theme	different	831:839	arg1	those					866:870	those	866:870	those	866:870	Although the enzyme was preferentially active with maltose, it was also able to hydrolyze different soluble starches such as those from potato, corn or rice.
35997915	5	11	theme	activities	629:638	arg1	activities					629:638	the highest specific activities	608:638	the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg)	608:688	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	11	theme	activities	629:638	arg1	one					601:603	one	601:603	one	601:603	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	7	12	theme	ethanol	1077:1083	arg1	concentrations					1059:1072	moderate concentrations	1050:1072	moderate concentrations of ethanol	1050:1083	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35997915	7	13	from	presence	1038:1045	arg1	stable					1024:1029	stable	1024:1029	stable	1024:1029	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35997915	7	14	theme	PEG8000	993:999	arg1	presence					981:988	the presence	977:988	the presence of PEG8000	977:999	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35997915	3	15	theme	culture	512:518	arg1	medium					520:525	culture medium	512:525	culture medium	512:525	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	0	16	theme	Thermoanaerobacter	118:135	arg1	ethanolicus					137:147	Thermoanaerobacter ethanolicus	118:147	Thermoanaerobacter ethanolicus	118:147	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	4	17	theme	optimum	556:562	arg1	temperature					564:574	a high optimum temperature	549:574	a high optimum temperature of 75 °C	549:583	RESULTS TeGA showed a high optimum temperature of 75 °C.
35997915	1	18	dep	PURPOSE	150:156	arg1	identified					161:170	identified	161:170	identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium	161:268	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	7	19	theme	moderate	1050:1057	arg1	concentrations					1059:1072	moderate concentrations	1050:1072	moderate concentrations of ethanol	1050:1083	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35997915	7	20	dep	to	937:938	arg1	up					934:935	up	934:935	up	934:935	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35997915	6	21	theme	soluble	841:847	arg1	starches					849:856	different soluble starches	831:856	different soluble starches such as those from potato, corn or rice	831:896	Although the enzyme was preferentially active with maltose, it was also able to hydrolyze different soluble starches such as those from potato, corn or rice.
35997915	6	21	theme	soluble	841:847	arg1	those					866:870	those	866:870	those	866:870	Although the enzyme was preferentially active with maltose, it was also able to hydrolyze different soluble starches such as those from potato, corn or rice.
35997915	2	22	theme	glycosylhydrolases	336:353	arg1	family					323:328	the family 15	319:331	the family 15 of glycosylhydrolases (GH15)	319:360	Structural studies suggest that TeGA belongs to the family 15 of glycosylhydrolases (GH15).
35997915	1	23	theme	Thermoanaerobacter	203:220	arg1	bacterium					260:268	a thermophilic anaerobic bacterium	235:268	a thermophilic anaerobic bacterium	235:268	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	1	23	theme	Thermoanaerobacter	203:220	arg1	ethanolicus					222:232	Thermoanaerobacter ethanolicus	203:232	Thermoanaerobacter ethanolicus	203:232	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	3	24	theme	enzyme	394:399	arg1	expression					375:384	The expression	371:384	The expression of this enzyme	371:399	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	8	25	theme	biofuel	1186:1192	arg1	production					1194:1203	biofuel production	1186:1203	biofuel production	1186:1203	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	0	26	theme	molecular	16:24	arg1	characterization					42:57	molecular and biochemical characterization	16:57	molecular and biochemical characterization of a novel thermoactive	16:81	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	3	27	theme	highest	460:466	arg1	amount					468:473	the highest amount	456:473	the highest amount of soluble protein	456:492	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	3	27	theme	highest	460:466	arg1	protein					486:492	soluble protein	478:492	soluble protein	478:492	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	5	28	from	stable	703:708	arg1	3.0-10.0					730:737	3.0-10.0	730:737	3.0-10.0	730:737	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	28	from	stable	703:708	arg1	range					723:727	a wide pH range	713:727	a wide pH range (3.0-10.0)	713:738	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	2	29	theme	Structural	271:280	arg1	studies					282:288	Structural studies	271:288	Structural studies	271:288	Structural studies suggest that TeGA belongs to the family 15 of glycosylhydrolases (GH15).
35997915	8	30	theme	industrial	1157:1166	arg1	processes					1168:1176	different industrial processes	1147:1176	different industrial processes such as biofuel production and food processing	1147:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	8	30	theme	industrial	1157:1166	arg1	production					1194:1203	biofuel production	1186:1203	biofuel production	1186:1203	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	8	30	theme	industrial	1157:1166	arg1	processing					1214:1223	food processing	1209:1223	food processing	1209:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	0	31	theme	biochemical	30:40	arg1	characterization					42:57	molecular and biochemical characterization	16:57	molecular and biochemical characterization of a novel thermoactive	16:81	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	3	32	theme	BL21	427:430	arg1	cells					433:437	E. coli (BL21) cells	418:437	E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium)	418:526	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	1	33	theme	thermophilic	237:248	arg1	bacterium					260:268	a thermophilic anaerobic bacterium	235:268	a thermophilic anaerobic bacterium	235:268	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	1	33	theme	thermophilic	237:248	arg1	ethanolicus					222:232	Thermoanaerobacter ethanolicus	203:232	Thermoanaerobacter ethanolicus	203:232	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	3	34	theme	E.	418:419	arg1	cells					433:437	E. coli (BL21) cells	418:437	E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium)	418:526	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	8	35	dep	CONCLUSION	1086:1095	arg1	propose					1100:1106	propose	1100:1106	propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing	1100:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	3	36	theme	soluble	478:484	arg1	protein					486:492	soluble protein	478:492	soluble protein	478:492	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	3	37	theme	coli	421:424	arg1	cells					433:437	E. coli (BL21) cells	418:437	E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium)	418:526	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	0	38	from	ethanolicus	137:147	arg1	characterization					42:57	molecular and biochemical characterization	16:57	molecular and biochemical characterization of a novel thermoactive	16:81	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	0	38	from	ethanolicus	137:147	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	0	38	from	ethanolicus	137:147	arg1	glucoamylase					100:111	thermostable glucoamylase	87:111	thermostable glucoamylase from Thermoanaerobacter ethanolicus	87:147	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	7	39	theme	high	913:916	arg1	thermostability					918:932	a high thermostability	911:932	a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol	911:1083	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35997915	3	40	theme	protein	486:492	arg1	amount					468:473	the highest amount	456:473	the highest amount of soluble protein	456:492	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	3	40	theme	protein	486:492	arg1	protein					486:492	soluble protein	478:492	soluble protein	478:492	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	5	41	theme	bacterial	655:663	arg1	glucoamylase					665:676	a bacterial glucoamylase	653:676	a bacterial glucoamylase (75.3 U/mg)	653:688	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	5	41	theme	bacterial	655:663	arg1	U/mg					684:687	75.3 U/mg	679:687	75.3 U/mg	679:687	It also showed one of the highest specific activities reported for a bacterial glucoamylase (75.3 U/mg) and was also stable in a wide pH range (3.0-10.0).
35997915	7	42	from	stable	1024:1029	arg1	presence					1038:1045	the presence	1034:1045	the presence of moderate concentrations of ethanol	1034:1083	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35997915	1	43	theme	anaerobic	250:258	arg1	bacterium					260:268	a thermophilic anaerobic bacterium	235:268	a thermophilic anaerobic bacterium	235:268	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	1	43	theme	anaerobic	250:258	arg1	ethanolicus					222:232	Thermoanaerobacter ethanolicus	203:232	Thermoanaerobacter ethanolicus	203:232	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	3	44	theme	medium	520:525	arg1	3 mg/l					502:507	3 mg/l	502:507	3 mg/l of culture medium	502:525	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	3	45	dep	have	451:454	arg1	3 mg/l					502:507	3 mg/l	502:507	3 mg/l of culture medium	502:525	METHODS The expression of this enzyme was optimized in E. coli (BL21) cells in order to have the highest amount of soluble protein (around 3 mg/l of culture medium).
35997915	4	46	theme	75 °C	579:583	arg1	temperature					564:574	a high optimum temperature	549:574	a high optimum temperature of 75 °C	549:583	RESULTS TeGA showed a high optimum temperature of 75 °C.
35997915	0	47	theme	thermoactive	70:81	arg1	characterization					42:57	molecular and biochemical characterization	16:57	molecular and biochemical characterization of a novel thermoactive	16:81	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	0	47	theme	thermoactive	70:81	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	0	47	theme	thermoactive	70:81	arg1	glucoamylase					100:111	thermostable glucoamylase	87:111	thermostable glucoamylase from Thermoanaerobacter ethanolicus	87:147	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	6	48	with	active	780:785	arg1	maltose					792:798	maltose	792:798	maltose	792:798	Although the enzyme was preferentially active with maltose, it was also able to hydrolyze different soluble starches such as those from potato, corn or rice.
35997915	8	49	theme	different	1147:1155	arg1	processes					1168:1176	different industrial processes	1147:1176	different industrial processes such as biofuel production and food processing	1147:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	8	49	theme	different	1147:1155	arg1	production					1194:1203	biofuel production	1186:1203	biofuel production	1186:1203	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	8	49	theme	different	1147:1155	arg1	processing					1214:1223	food processing	1209:1223	food processing	1209:1223	CONCLUSION We propose that TeGA could be suitable for use in different industrial processes such as biofuel production and food processing.
35997915	0	50	theme	novel	64:68	arg1	thermoactive					70:81	a novel thermoactive	62:81	a novel thermoactive	62:81	Identification, molecular and biochemical characterization of a novel thermoactive and thermostable glucoamylase from Thermoanaerobacter ethanolicus.
35997915	1	51	theme	new	174:176	arg1	TeGA					192:195	TeGA	192:195	TeGA	192:195	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	1	51	theme	new	174:176	arg1	glucoamylase					178:189	a new glucoamylase	172:189	a new glucoamylase (TeGA)	172:196	PURPOSE We identified a new glucoamylase (TeGA) from Thermoanaerobacter ethanolicus, a thermophilic anaerobic bacterium.
35997915	7	52	theme	concentrations	1059:1072	arg1	presence					1038:1045	the presence	1034:1045	the presence of moderate concentrations of ethanol	1034:1083	TeGA showed a high thermostability up to around 70 °C, which was increased in the presence of PEG8000, and also showed to be stable in the presence of moderate concentrations of ethanol.
35839881	10	0	theme	extracellular	1544:1556	arg1	EPS					1580:1582	EPS	1580:1582	EPS	1580:1582	The extracellular polymeric substances (EPS) content and analysis showed that entrapment does not change the composition of EPS, and can maintain the EPS content.
35839881	10	0	theme	extracellular	1544:1556	arg1	substances					1568:1577	extracellular polymeric substances	1544:1577	The extracellular polymeric substances (EPS) content	1540:1591	The extracellular polymeric substances (EPS) content and analysis showed that entrapment does not change the composition of EPS, and can maintain the EPS content.
35839881	4	1	theme	nano	469:472	arg1	beads					498:502	polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads	430:502	beads	498:502	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	9	2	theme	nano	1401:1404	arg1	Fe2O3					1406:1410	nano Fe2O3	1401:1410	nano Fe2O3	1401:1410	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	9	3	theme	activity	1530:1537	arg1	reduction					1480:1488	the reduction	1476:1488	the reduction of hydrazine dehydrogenase (HDH) enzyme activity	1476:1537	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	4	4	theme	anammox	570:576	arg1	sludge					578:583	entrapment enhanced anammox sludge	550:583	entrapment enhanced anammox sludge	550:583	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	12	5	theme	quantity	1998:2005	arg1	"					2006:2006	"quantity"	1997:2006	"quantity"	1997:2006	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	9	6	dep	stimulate	1416:1424	arg1	beneficial					1337:1346	beneficial	1337:1346	beneficial	1337:1346	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	2	7	theme	oxidation	331:339	arg1	application					298:308	the mainstream application	283:308	the mainstream application of anaerobic ammonia oxidation (anammox)	283:349	In suspended systems, sludge is easily lost, which limits the mainstream application of anaerobic ammonia oxidation (anammox).
35839881	9	8	theme	hydrazine	1493:1501	arg1	HDH					1518:1520	HDH	1518:1520	HDH	1518:1520	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	9	8	theme	hydrazine	1493:1501	arg1	dehydrogenase					1503:1515	hydrazine dehydrogenase	1493:1515	hydrazine dehydrogenase (HDH) enzyme activity	1493:1537	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	4	9	theme	enhanced	561:568	arg1	sludge					578:583	entrapment enhanced anammox sludge	550:583	entrapment enhanced anammox sludge	550:583	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	8	10	theme	denitrifying	1180:1191	arg1	DNB					1203:1205	DNB	1203:1205	DNB	1203:1205	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	8	10	theme	denitrifying	1180:1191	arg1	bacteria					1193:1200	denitrifying bacteria	1180:1200	denitrifying bacteria (DNB)	1180:1206	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	4	11	theme	entrapment	487:496	arg1	beads					498:502	polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads	430:502	beads	498:502	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	7	12	theme	highest	981:987	arg1	degree					989:994	the highest degree	977:994	the highest degree of recovery	977:1006	Nano Fe2O3-PVA-SA entrapped beads showed the smallest decline and the highest degree of recovery.
35839881	6	13	theme	entrapped	809:817	arg1	granules					770:777	granules	770:777	granules	770:777	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	6	13	theme	entrapped	809:817	arg1	beads					819:823	nano Fe2O3-PVA-SA entrapped beads	791:823	nano Fe2O3-PVA-SA entrapped beads	791:823	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	0	14	theme	nano	84:87	arg1	Fe2O3-PVA-SA					89:100	nano Fe2O3-PVA-SA	84:100	nano Fe2O3-PVA-SA	84:100	Activity enhancement and the anammox mechanism under low temperature via PVA-SA and nano Fe2O3-PVA-SA entrapped beads.
35839881	1	15	contain	have	163:166	arg2	activity					195:202	low activity	191:202	low activity	191:202	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	1	15	contain	have	163:166	arg1	bacteria					147:154	Anaerobic ammonia-oxidizing bacteria	119:154	Anaerobic ammonia-oxidizing bacteria (AAOB)	119:161	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	1	15	contain	have	163:166	arg2	time					182:185	a long growth time	168:185	a long growth time	168:185	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	1	15	contain	have	163:166	arg1	AAOB					157:160	AAOB	157:160	AAOB	157:160	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	6	16	theme	Fe2O3-PVA-SA	796:807	arg1	granules					770:777	granules	770:777	granules	770:777	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	6	16	theme	Fe2O3-PVA-SA	796:807	arg1	beads					819:823	nano Fe2O3-PVA-SA entrapped beads	791:823	nano Fe2O3-PVA-SA entrapped beads	791:823	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	12	17	theme	genes	1866:1870	arg1	expression					1841:1850	the expression	1837:1850	the expression of functional genes	1837:1870	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	4	18	theme	sludge	578:583	arg1	effectiveness					533:545	the effectiveness	529:545	the effectiveness of entrapment enhanced anammox sludge	529:583	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	8	19	theme	Reaction	1009:1016	arg1	ratio					1027:1031	Reaction metering ratio	1009:1031	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N)	1009:1069	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	6	20	theme	low-temperature	884:898	arg1	operation					900:908	low-temperature operation	884:908	low-temperature operation	884:908	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	6	21	theme	nano	791:794	arg1	granules					770:777	granules	770:777	granules	770:777	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	6	21	theme	nano	791:794	arg1	beads					819:823	nano Fe2O3-PVA-SA entrapped beads	791:823	nano Fe2O3-PVA-SA entrapped beads	791:823	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	8	22	dep	ratio	1027:1031	arg1	△NO3--N/△NH4+-N					1054:1068	△NO3--N/△NH4+-N	1054:1068	△NO3--N/△NH4+-N	1054:1068	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	8	22	dep	ratio	1027:1031	arg1	△NO2--N/△NH4+-N					1034:1048	△NO2--N/△NH4+-N	1034:1048	△NO2--N/△NH4+-N	1034:1048	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	10	23	theme	EPS	1664:1666	arg1	composition					1649:1659	the composition	1645:1659	the composition of EPS	1645:1666	The extracellular polymeric substances (EPS) content and analysis showed that entrapment does not change the composition of EPS, and can maintain the EPS content.
35839881	8	24	theme	bacteria	1193:1200	arg1	activity					1168:1175	the activity	1164:1175	the activity of denitrifying bacteria (DNB)	1164:1206	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	10	25	theme	EPS	1690:1692	arg1	content					1694:1700	the EPS content	1686:1700	the EPS content	1686:1700	The extracellular polymeric substances (EPS) content and analysis showed that entrapment does not change the composition of EPS, and can maintain the EPS content.
35839881	8	26	theme	metering	1018:1025	arg1	ratio					1027:1031	Reaction metering ratio	1009:1031	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N)	1009:1069	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	3	27	theme	effective	371:379	arg1	ideas					381:385	effective ideas	371:385	effective ideas	371:385	Entrapment provides effective ideas for solving these problems.
35839881	11	28	theme	sludge	1765:1770	arg1	stability					1772:1780	sludge stability	1765:1780	sludge stability	1765:1780	Nano Fe2O3 can stimulate AAOB to secrete more EPS to maintain sludge stability.
35839881	1	29	theme	long	170:173	arg1	time					182:185	a long growth time	168:185	a long growth time	168:185	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	7	30	theme	smallest	956:963	arg1	decline					965:971	the smallest decline	952:971	the smallest decline	952:971	Nano Fe2O3-PVA-SA entrapped beads showed the smallest decline and the highest degree of recovery.
35839881	2	31	theme	mainstream	287:296	arg1	application					298:308	the mainstream application	283:308	the mainstream application of anaerobic ammonia oxidation (anammox)	283:349	In suspended systems, sludge is easily lost, which limits the mainstream application of anaerobic ammonia oxidation (anammox).
35839881	1	32	theme	growth	175:180	arg1	time					182:185	a long growth time	168:185	a long growth time	168:185	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	0	33	theme	Activity	0:7	arg1	enhancement					9:19	Activity enhancement	0:19	Activity enhancement	0:19	Activity enhancement and the anammox mechanism under low temperature via PVA-SA and nano Fe2O3-PVA-SA entrapped beads.
35839881	5	34	theme	entrapped	629:637	arg1	beads					639:643	the entrapped beads	625:643	the entrapped beads	625:643	The differences in the entrapped beads and granules were compared to analyze the strengthening mechanism.
35839881	12	35	theme	"	2006:2006	arg1	perspectives					1967:1978	the dual perspectives	1958:1978	the dual perspectives of "quality" and "quantity"	1958:2006	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	8	36	theme	strict	1264:1269	arg1	environment					1281:1291	a strict anaerobic environment	1262:1291	a strict anaerobic environment	1262:1291	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	12	37	theme	AAOB	1899:1902	arg1	enrichment					1885:1894	the enrichment	1881:1894	the enrichment of AAOB	1881:1902	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	12	38	dep	maintain	1828:1835	arg1	promote					1873:1879	promote	1873:1879	promote the enrichment of AAOB	1873:1902	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	5	39	theme	strengthening	687:699	arg1	mechanism					701:709	the strengthening mechanism	683:709	the strengthening mechanism	683:709	The differences in the entrapped beads and granules were compared to analyze the strengthening mechanism.
35839881	6	40	theme	granules	770:777	arg1	performance					755:765	the nitrogen removal performance	734:765	the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads,	734:824	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	8	41	theme	anaerobic	1271:1279	arg1	environment					1281:1291	a strict anaerobic environment	1262:1291	a strict anaerobic environment	1262:1291	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	12	42	theme	dual	1962:1965	arg1	perspectives					1967:1978	the dual perspectives	1958:1978	the dual perspectives of "quality" and "quantity"	1958:2006	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	4	43	theme	entrapment	550:559	arg1	sludge					578:583	entrapment enhanced anammox sludge	550:583	entrapment enhanced anammox sludge	550:583	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	12	44	from	perspectives	1967:1978	arg1	performance					1941:1951	the nitrogen removal performance	1920:1951	the nitrogen removal performance from the dual perspectives of "quality" and "quantity"	1920:2006	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	10	45	theme	polymeric	1558:1566	arg1	EPS					1580:1582	EPS	1580:1582	EPS	1580:1582	The extracellular polymeric substances (EPS) content and analysis showed that entrapment does not change the composition of EPS, and can maintain the EPS content.
35839881	10	45	theme	polymeric	1558:1566	arg1	substances					1568:1577	extracellular polymeric substances	1544:1577	The extracellular polymeric substances (EPS) content	1540:1591	The extracellular polymeric substances (EPS) content and analysis showed that entrapment does not change the composition of EPS, and can maintain the EPS content.
35839881	11	46	theme	Nano	1703:1706	arg1	Fe2O3					1708:1712	Nano Fe2O3	1703:1712	Nano Fe2O3	1703:1712	Nano Fe2O3 can stimulate AAOB to secrete more EPS to maintain sludge stability.
35839881	1	47	theme	low	191:193	arg1	activity					195:202	low activity	191:202	low activity	191:202	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	12	48	theme	nitrogen	1924:1931	arg1	performance					1941:1951	the nitrogen removal performance	1920:1951	the nitrogen removal performance from the dual perspectives of "quality" and "quantity"	1920:2006	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	2	49	theme	anaerobic	313:321	arg1	anammox					342:348	anammox	342:348	anammox	342:348	In suspended systems, sludge is easily lost, which limits the mainstream application of anaerobic ammonia oxidation (anammox).
35839881	2	49	theme	anaerobic	313:321	arg1	oxidation					331:339	anaerobic ammonia oxidation	313:339	anaerobic ammonia oxidation (anammox)	313:349	In suspended systems, sludge is easily lost, which limits the mainstream application of anaerobic ammonia oxidation (anammox).
35839881	8	50	theme	bacteria	1126:1133	arg1	inhibition					1141:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	6	51	dep	cooling	872:878	arg1	the					868:870	the	868:870	the	868:870	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	5	52	from	differences	610:620	arg1	granules					649:656	granules	649:656	granules	649:656	The differences in the entrapped beads and granules were compared to analyze the strengthening mechanism.
35839881	5	52	from	differences	610:620	arg1	beads					639:643	the entrapped beads	625:643	the entrapped beads	625:643	The differences in the entrapped beads and granules were compared to analyze the strengthening mechanism.
35839881	0	53	theme	anammox	29:35	arg1	mechanism					37:45	the anammox mechanism	25:45	the anammox mechanism	25:45	Activity enhancement and the anammox mechanism under low temperature via PVA-SA and nano Fe2O3-PVA-SA entrapped beads.
35839881	9	54	theme	heme	1379:1382	arg1	c					1384:1384	heme c	1379:1384	heme c	1379:1384	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	8	55	theme	oxidizing	1116:1124	arg1	inhibition					1141:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	4	56	theme	alginate	447:454	arg1	PVA-SA					457:462	polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads	430:502	PVA-SA	457:462	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	12	57	theme	quality	1984:1990	arg1	"					1991:1991	"quality"	1983:1991	"quality"	1983:1991	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	0	58	theme	low	53:55	arg1	temperature					57:67	low temperature	53:67	low temperature	53:67	Activity enhancement and the anammox mechanism under low temperature via PVA-SA and nano Fe2O3-PVA-SA entrapped beads.
35839881	9	59	theme	c	1384:1384	arg1	content					1368:1374	the content	1364:1374	the content of heme c	1364:1384	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	8	60	theme	NOB	1136:1138	arg1	inhibition					1141:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	4	61	theme	polyvinyl‑sodium	430:445	arg1	PVA-SA					457:462	polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads	430:502	PVA-SA	457:462	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	12	62	theme	molecular	1790:1798	arg1	perspective					1800:1810	a molecular perspective	1788:1810	a molecular perspective	1788:1810	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	2	63	theme	ammonia	323:329	arg1	anammox					342:348	anammox	342:348	anammox	342:348	In suspended systems, sludge is easily lost, which limits the mainstream application of anaerobic ammonia oxidation (anammox).
35839881	2	63	theme	ammonia	323:329	arg1	oxidation					331:339	anaerobic ammonia oxidation	313:339	anaerobic ammonia oxidation (anammox)	313:349	In suspended systems, sludge is easily lost, which limits the mainstream application of anaerobic ammonia oxidation (anammox).
35839881	12	64	theme	removal	1933:1939	arg1	performance					1941:1951	the nitrogen removal performance	1920:1951	the nitrogen removal performance from the dual perspectives of "quality" and "quantity"	1920:2006	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	8	65	theme	Nitrite	1108:1114	arg1	inhibition					1141:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Nitrite oxidizing bacteria (NOB) inhibition	1108:1150	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	12	66	theme	"	1991:1991	arg1	perspectives					1967:1978	the dual perspectives	1958:1978	the dual perspectives of "quality" and "quantity"	1958:2006	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	6	67	theme	removal	747:753	arg1	performance					755:765	the nitrogen removal performance	734:765	the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads,	734:824	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	1	68	theme	Anaerobic	119:127	arg1	AAOB					157:160	AAOB	157:160	AAOB	157:160	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	1	68	theme	Anaerobic	119:127	arg1	bacteria					147:154	Anaerobic ammonia-oxidizing bacteria	119:154	Anaerobic ammonia-oxidizing bacteria (AAOB)	119:161	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	7	69	theme	Fe2O3-PVA-SA	916:927	arg1	beads					939:943	Nano Fe2O3-PVA-SA entrapped beads	911:943	Nano Fe2O3-PVA-SA entrapped beads	911:943	Nano Fe2O3-PVA-SA entrapped beads showed the smallest decline and the highest degree of recovery.
35839881	6	70	theme	nitrogen	738:745	arg1	performance					755:765	the nitrogen removal performance	734:765	the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads,	734:824	The results show that the nitrogen removal performance of granules, PVA-SA and nano Fe2O3-PVA-SA entrapped beads, first decreased and then increased during the cooling and low-temperature operation.
35839881	1	71	theme	ammonia-oxidizing	129:145	arg1	AAOB					157:160	AAOB	157:160	AAOB	157:160	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	1	71	theme	ammonia-oxidizing	129:145	arg1	bacteria					147:154	Anaerobic ammonia-oxidizing bacteria	119:154	Anaerobic ammonia-oxidizing bacteria (AAOB)	119:161	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	7	72	theme	entrapped	929:937	arg1	beads					939:943	Nano Fe2O3-PVA-SA entrapped beads	911:943	Nano Fe2O3-PVA-SA entrapped beads	911:943	Nano Fe2O3-PVA-SA entrapped beads showed the smallest decline and the highest degree of recovery.
35839881	1	73	theme	low	207:209	arg1	temperatures					211:222	low temperatures	207:222	low temperatures	207:222	Anaerobic ammonia-oxidizing bacteria (AAOB) have a long growth time and low activity at low temperatures.
35839881	8	74	theme	nitrogen	1240:1247	arg1	removal					1223:1229	the removal	1219:1229	the removal of total nitrogen	1219:1247	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	7	75	theme	recovery	999:1006	arg1	degree					989:994	the highest degree	977:994	the highest degree of recovery	977:1006	Nano Fe2O3-PVA-SA entrapped beads showed the smallest decline and the highest degree of recovery.
35839881	7	75	theme	recovery	999:1006	arg1	decline					965:971	the smallest decline	952:971	the smallest decline	952:971	Nano Fe2O3-PVA-SA entrapped beads showed the smallest decline and the highest degree of recovery.
35839881	12	76	theme	functional	1855:1864	arg1	genes					1866:1870	functional genes	1855:1870	functional genes	1855:1870	From a molecular perspective, entrapment can maintain the expression of functional genes, promote the enrichment of AAOB, thus improving the nitrogen removal performance from the dual perspectives of "quality" and "quantity".
35839881	4	77	theme	low	588:590	arg1	temperatures					592:603	low temperatures	588:603	low temperatures	588:603	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	10	78	theme	substances	1568:1577	arg1	content					1585:1591	The extracellular polymeric substances (EPS) content	1540:1591	The extracellular polymeric substances (EPS) content	1540:1591	The extracellular polymeric substances (EPS) content and analysis showed that entrapment does not change the composition of EPS, and can maintain the EPS content.
35839881	9	79	theme	dehydrogenase	1503:1515	arg1	activity					1530:1537	hydrazine dehydrogenase (HDH) enzyme activity	1493:1537	hydrazine dehydrogenase (HDH) enzyme activity	1493:1537	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
35839881	7	80	theme	Nano	911:914	arg1	beads					939:943	Nano Fe2O3-PVA-SA entrapped beads	911:943	Nano Fe2O3-PVA-SA entrapped beads	911:943	Nano Fe2O3-PVA-SA entrapped beads showed the smallest decline and the highest degree of recovery.
35839881	8	81	theme	total	1234:1238	arg1	nitrogen					1240:1247	total nitrogen	1234:1247	total nitrogen	1234:1247	Reaction metering ratio (△NO2--N/△NH4+-N and △NO3--N/△NH4+-N) showed that entrapment could realize Nitrite oxidizing bacteria (NOB) inhibition and improve the activity of denitrifying bacteria (DNB) to promote the removal of total nitrogen by providing a strict anaerobic environment.
35839881	4	82	theme	Fe2O3-PVA-SA	474:485	arg1	beads					498:502	polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads	430:502	beads	498:502	In this study, polyvinyl‑sodium alginate (PVA-SA) and nano Fe2O3-PVA-SA entrapment beads were prepared to discuss the effectiveness of entrapment enhanced anammox sludge at low temperatures.
35839881	2	83	theme	suspended	228:236	arg1	systems					238:244	suspended systems	228:244	suspended systems	228:244	In suspended systems, sludge is easily lost, which limits the mainstream application of anaerobic ammonia oxidation (anammox).
35839881	9	84	theme	enzyme	1523:1528	arg1	activity					1530:1537	hydrazine dehydrogenase (HDH) enzyme activity	1493:1537	hydrazine dehydrogenase (HDH) enzyme activity	1493:1537	The results demonstrate that entrapment is beneficial for maintaining the content of heme c, specifically, nano Fe2O3 can stimulate its production, and is beneficial for alleviating the reduction of hydrazine dehydrogenase (HDH) enzyme activity.
37126504	8	0	theme	innate	1287:1292	arg1	response					1301:1308	the innate immune response	1283:1308	the innate immune response	1283:1308	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	5	1	theme	fungal	698:703	arg1	clearance					705:713	fungal clearance	698:713	fungal clearance	698:713	ZfpA deletion promotes fungal clearance and attenuates virulence in wild-type hosts and this virulence defect is abrogated in neutrophil-deficient zebrafish.
37126504	4	2	theme	human	563:567	arg1	neutrophils					569:579	primary human neutrophils	555:579	primary human neutrophils	555:579	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	7	3	from	susceptibility	1157:1170	arg1	zebrafish					1196:1204	neutrophil-deficient zebrafish	1175:1204	neutrophil-deficient zebrafish	1175:1204	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	7	4	theme	ZfpA	969:972	arg1	Overexpression					951:964	Overexpression	951:964	Overexpression of ZfpA	951:972	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	3	5	theme	fungal	395:400	arg1	growth					402:407	fungal growth	395:407	fungal growth	395:407	However, how ZfpA affects fungal growth and susceptibility to host immunity during infection has not been investigated.
37126504	4	6	theme	primary	555:561	arg1	neutrophils					569:579	primary human neutrophils	555:579	primary human neutrophils	555:579	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	8	7	theme	immune	1294:1299	arg1	response					1301:1308	the innate immune response	1283:1308	the innate immune response	1283:1308	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	4	8	theme	larval	506:511	arg1	model					545:549	the larval zebrafish-Aspergillus infection model	502:549	the larval zebrafish-Aspergillus infection model	502:549	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	7	9	theme	hyphal	1066:1071	arg1	development					1073:1083	hyphal development	1066:1083	hyphal development	1066:1083	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	8	10	theme	antifungal	1314:1323	arg1	treatment					1325:1333	antifungal treatment	1314:1333	antifungal treatment during A. fumigatus infection	1314:1363	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	4	11	dep	A.	607:608	arg1	fumigatus					610:618	fumigatus	610:618	fumigatus	610:618	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	6	12	theme	human	880:884	arg1	neutrophils					886:896	human neutrophils	880:896	human neutrophils	880:896	ZfpA deletion also increases susceptibility to human neutrophils ex vivo while overexpression impairs fungal killing.
37126504	8	13	theme	A.	1342:1343	arg1	infection					1355:1363	A. fumigatus infection	1342:1363	A. fumigatus infection	1342:1363	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	1	14	theme	host	190:193	arg1	colonization					195:206	host colonization	190:206	host colonization during Aspergillus infection	190:235	Hyphal growth is essential for host colonization during Aspergillus infection.
37126504	0	15	theme	neutrophil	122:131	arg1	killing					133:139	neutrophil killing	122:139	neutrophil killing	122:139	Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection.
37126504	4	16	theme	infection	535:543	arg1	model					545:549	the larval zebrafish-Aspergillus infection model	502:549	the larval zebrafish-Aspergillus infection model	502:549	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	5	17	theme	ZfpA	675:678	arg1	deletion					680:687	ZfpA deletion	675:687	ZfpA deletion	675:687	ZfpA deletion promotes fungal clearance and attenuates virulence in wild-type hosts and this virulence defect is abrogated in neutrophil-deficient zebrafish.
37126504	0	18	theme	Aspergillus	0:10	arg1	ZfpA					43:46	Aspergillus fumigatus transcription factor ZfpA	0:46	Aspergillus fumigatus transcription factor ZfpA	0:46	Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection.
37126504	7	19	theme	caspofungin	1145:1155	arg1	susceptibility					1157:1170	caspofungin susceptibility	1145:1170	caspofungin susceptibility in neutrophil-deficient zebrafish	1145:1204	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	4	20	theme	zebrafish-Aspergillus	513:533	arg1	model					545:549	the larval zebrafish-Aspergillus infection model	502:549	the larval zebrafish-Aspergillus infection model	502:549	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	8	21	dep	A.	1342:1343	arg1	fumigatus					1345:1353	fumigatus	1345:1353	fumigatus	1345:1353	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	3	22	theme	host	431:434	arg1	immunity					436:443	host immunity	431:443	host immunity	431:443	However, how ZfpA affects fungal growth and susceptibility to host immunity during infection has not been investigated.
37126504	2	23	theme	wall	351:354	arg1	composition					356:366	cell wall composition	346:366	cell wall composition	346:366	The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition.
37126504	1	24	theme	Aspergillus	215:225	arg1	infection					227:235	Aspergillus infection	215:235	Aspergillus infection	215:235	Hyphal growth is essential for host colonization during Aspergillus infection.
37126504	0	25	theme	factor	36:41	arg1	ZfpA					43:46	Aspergillus fumigatus transcription factor ZfpA	0:46	Aspergillus fumigatus transcription factor ZfpA	0:46	Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection.
37126504	7	26	theme	chitin	1042:1047	arg1	synthesis					1049:1057	chitin synthesis	1042:1057	chitin synthesis	1042:1057	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	2	27	theme	cell	346:349	arg1	composition					356:366	cell wall composition	346:366	cell wall composition	346:366	The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition.
37126504	0	28	theme	transcription	22:34	arg1	ZfpA					43:46	Aspergillus fumigatus transcription factor ZfpA	0:46	Aspergillus fumigatus transcription factor ZfpA	0:46	Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection.
37126504	6	29	theme	fungal	935:940	arg1	killing					942:948	fungal killing	935:948	fungal killing	935:948	ZfpA deletion also increases susceptibility to human neutrophils ex vivo while overexpression impairs fungal killing.
37126504	4	30	theme	A.	607:608	arg1	pathogenesis					620:631	A. fumigatus pathogenesis	607:631	A. fumigatus pathogenesis	607:631	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	5	31	theme	virulence	768:776	arg1	defect					778:783	this virulence defect	763:783	this virulence defect	763:783	ZfpA deletion promotes fungal clearance and attenuates virulence in wild-type hosts and this virulence defect is abrogated in neutrophil-deficient zebrafish.
37126504	2	32	theme	A.	278:279	arg1	development					298:308	A. fumigatus hyphal development	278:308	A. fumigatus hyphal development	278:308	The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition.
37126504	2	33	dep	A.	278:279	arg1	fumigatus					281:289	fumigatus	281:289	fumigatus	281:289	The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition.
37126504	8	34	theme	protective	1232:1241	arg1	role					1243:1246	a protective role	1230:1246	a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection	1230:1363	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	5	35	theme	neutrophil-deficient	801:820	arg1	zebrafish					822:830	neutrophil-deficient zebrafish	801:830	neutrophil-deficient zebrafish	801:830	ZfpA deletion promotes fungal clearance and attenuates virulence in wild-type hosts and this virulence defect is abrogated in neutrophil-deficient zebrafish.
37126504	8	36	theme	ZfpA	1252:1255	arg1	activity					1257:1264	ZfpA activity	1252:1264	ZfpA activity	1252:1264	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	6	37	theme	ZfpA	833:836	arg1	deletion					838:845	ZfpA deletion	833:845	ZfpA deletion	833:845	ZfpA deletion also increases susceptibility to human neutrophils ex vivo while overexpression impairs fungal killing.
37126504	0	38	theme	hyphal	58:63	arg1	development					65:75	hyphal development	58:75	hyphal development	58:75	Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection.
37126504	7	39	theme	neutrophil-deficient	1175:1194	arg1	zebrafish					1196:1204	neutrophil-deficient zebrafish	1175:1204	neutrophil-deficient zebrafish	1175:1204	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	2	40	theme	transcription	242:254	arg1	ZfpA					263:266	The transcription factor ZfpA	238:266	The transcription factor ZfpA	238:266	The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition.
37126504	2	41	theme	factor	256:261	arg1	ZfpA					263:266	The transcription factor ZfpA	238:266	The transcription factor ZfpA	238:266	The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition.
37126504	7	42	theme	wall	1119:1122	arg1	chitin					1124:1129	cell wall chitin	1114:1129	cell wall chitin	1114:1129	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	5	43	theme	wild-type	743:751	arg1	hosts					753:757	wild-type hosts	743:757	wild-type hosts	743:757	ZfpA deletion promotes fungal clearance and attenuates virulence in wild-type hosts and this virulence defect is abrogated in neutrophil-deficient zebrafish.
37126504	8	44	from	role	1243:1246	arg1	treatment					1325:1333	antifungal treatment	1314:1333	antifungal treatment during A. fumigatus infection	1314:1363	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	8	44	from	role	1243:1246	arg1	resistance					1269:1278	resistance	1269:1278	resistance to the innate immune response	1269:1308	These findings suggest a protective role for ZfpA activity in resistance to the innate immune response and antifungal treatment during A. fumigatus infection.
37126504	1	45	theme	Hyphal	159:164	arg1	growth					166:171	Hyphal growth	159:171	Hyphal growth	159:171	Hyphal growth is essential for host colonization during Aspergillus infection.
37126504	4	46	theme	antifungal	649:658	arg1	drugs					660:664	antifungal drugs	649:664	antifungal drugs	649:664	Here, we use the larval zebrafish-Aspergillus infection model and primary human neutrophils to probe how ZfpA affects A. fumigatus pathogenesis and response to antifungal drugs in vivo.
37126504	2	47	theme	hyphal	291:296	arg1	development					298:308	A. fumigatus hyphal development	278:308	A. fumigatus hyphal development	278:308	The transcription factor ZfpA regulates A. fumigatus hyphal development including branching, septation, and cell wall composition.
37126504	7	48	theme	cell	1114:1117	arg1	chitin					1124:1129	cell wall chitin	1114:1129	cell wall chitin	1114:1129	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	7	49	theme	antifungal	1005:1014	arg1	caspofungin					1016:1026	the antifungal caspofungin	1001:1026	the antifungal caspofungin	1001:1026	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37126504	5	50	from	virulence	730:738	arg1	hosts					753:757	wild-type hosts	743:757	wild-type hosts	743:757	ZfpA deletion promotes fungal clearance and attenuates virulence in wild-type hosts and this virulence defect is abrogated in neutrophil-deficient zebrafish.
37126504	0	51	dep	Aspergillus	0:10	arg1	fumigatus					12:20	fumigatus	12:20	fumigatus	12:20	Aspergillus fumigatus transcription factor ZfpA regulates hyphal development and alters susceptibility to antifungals and neutrophil killing during infection.
37126504	7	52	theme	ZfpA	1092:1095	arg1	deletion					1097:1104	ZfpA deletion	1092:1104	ZfpA deletion	1092:1104	Overexpression of ZfpA confers protection against the antifungal caspofungin by increasing chitin synthesis during hyphal development, while ZfpA deletion reduces cell wall chitin and increases caspofungin susceptibility in neutrophil-deficient zebrafish.
37087279	0	0	theme	dietary	76:82	arg1	fiber					67:71	NUTRIOSE® soluble fiber	49:71	NUTRIOSE® soluble fiber	49:71	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	0	0	theme	dietary	76:82	arg1	ingredient					84:93	a dietary ingredient	74:93	a dietary ingredient exhibiting prebiotic properties	74:125	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	7	1	theme	calorie	1133:1139	arg1	diet					1141:1144	a high calorie diet	1126:1144	a high calorie diet	1126:1144	Indeed, WT mice are protected from body weight gain and NAFLD induced by a high calorie diet.
37087279	9	2	theme	gut	1301:1303	arg1	composition					1316:1326	the gut microbiota composition	1297:1326	the gut microbiota composition	1297:1326	While the gut microbiota composition changes with NUTRIOSE®, this is not sufficient in itself to account for the benefits observed.
37087279	10	3	from	benefits	1475:1482	arg1	obligatory					1447:1456	obligatory	1447:1456	obligatory	1447:1456	On the contrary, IGN is obligatory in the NUTRIOSE® benefits, since no benefit take place in absence of IGN.
37087279	11	4	theme	crucial	1559:1565	arg1	role					1581:1584	a crucial and essential role	1557:1584	a crucial and essential role	1557:1584	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	5	5	from	role	858:861	arg1	effects					886:892	the observed effects	873:892	the observed effects	873:892	To assert the role of IGN in the observed effects, we studied wild-type (WT) and IGN-deficient mice.
37087279	2	6	theme	food	360:363	arg1	ingredient					365:374	a plant-based food ingredient	346:374	a plant-based food ingredient with beneficial effects in Humans	346:408	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	2	6	theme	food	360:363	arg1	fiber					337:341	NUTRIOSE® soluble fiber	319:341	NUTRIOSE® soluble fiber	319:341	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	3	7	from	function	541:548	arg1	effects					568:574	the beneficial effects	553:574	the beneficial effects of dietary fibers	553:592	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	9	8	theme	microbiota	1305:1314	arg1	composition					1316:1326	the gut microbiota composition	1297:1326	the gut microbiota composition	1297:1326	While the gut microbiota composition changes with NUTRIOSE®, this is not sufficient in itself to account for the benefits observed.
37087279	11	9	theme	effects	1618:1624	arg1	set-up					1593:1598	the set-up	1589:1598	the set-up of the beneficial effects of NUTRIOSE®	1589:1637	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	5	10	theme	IGN-deficient	925:937	arg1	mice					939:942	wild-type (WT) and IGN-deficient mice	906:942	wild-type (WT) and IGN-deficient mice	906:942	To assert the role of IGN in the observed effects, we studied wild-type (WT) and IGN-deficient mice.
37087279	11	11	theme	obesity	1752:1758	arg1	context					1729:1735	the context	1725:1735	the context of the current obesity epidemic	1725:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	5	12	theme	wild-type	906:914	arg1	mice					939:942	wild-type (WT) and IGN-deficient mice	906:942	wild-type (WT) and IGN-deficient mice	906:942	To assert the role of IGN in the observed effects, we studied wild-type (WT) and IGN-deficient mice.
37087279	8	13	theme	white	1269:1273	arg1	tissue					1283:1288	subcutaneous white adipose tissue	1256:1288	subcutaneous white adipose tissue	1256:1288	In addition, our data suggests that NUTRIOSE® may improve energy balance by activating a browning process in subcutaneous white adipose tissue.
37087279	8	14	theme	subcutaneous	1256:1267	arg1	tissue					1283:1288	subcutaneous white adipose tissue	1256:1288	subcutaneous white adipose tissue	1256:1288	In addition, our data suggests that NUTRIOSE® may improve energy balance by activating a browning process in subcutaneous white adipose tissue.
37087279	8	15	theme	browning	1236:1243	arg1	process					1245:1251	a browning process	1234:1251	a browning process	1234:1251	In addition, our data suggests that NUTRIOSE® may improve energy balance by activating a browning process in subcutaneous white adipose tissue.
37087279	11	16	theme	essential	1571:1579	arg1	role					1581:1584	a crucial and essential role	1557:1584	a crucial and essential role	1557:1584	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	4	17	theme	obesity	749:755	arg1	development					734:744	the development	730:744	the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent	730:841	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	5	18	theme	observed	877:884	arg1	effects					886:892	the observed effects	873:892	the observed effects	873:892	To assert the role of IGN in the observed effects, we studied wild-type (WT) and IGN-deficient mice.
37087279	7	19	theme	weight	1093:1098	arg1	gain					1100:1103	body weight gain	1088:1103	body weight gain	1088:1103	Indeed, WT mice are protected from body weight gain and NAFLD induced by a high calorie diet.
37087279	3	20	theme	intestinal	493:502	arg1	gluconeogenesis					504:518	intestinal gluconeogenesis	493:518	intestinal gluconeogenesis	493:518	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	11	21	with	supplementation	1673:1687	arg1	ingredients					1710:1720	healthy ingredients	1702:1720	healthy ingredients in the context of the current obesity epidemic	1702:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	6	22	theme	WT	1013:1014	arg1	mice					1016:1019	WT mice	1013:1019	WT mice	1013:1019	In line with our hypothesis, NUTRIOSE® exerts metabolic benefits in WT mice, but not in IGN-deficient mice.
37087279	0	23	theme	prebiotic	106:114	arg1	properties					116:125	prebiotic properties	106:125	prebiotic properties	106:125	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	6	24	theme	IGN-deficient	1033:1045	arg1	mice					1047:1050	IGN-deficient mice	1033:1050	IGN-deficient mice	1033:1050	In line with our hypothesis, NUTRIOSE® exerts metabolic benefits in WT mice, but not in IGN-deficient mice.
37087279	2	25	from	ingredient	365:374	arg1	context					310:316	this context	305:316	this context	305:316	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	3	26	theme	essential	531:539	arg1	function					541:548	the essential function	527:548	the essential function in the beneficial effects of dietary fibers	527:592	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	3	26	theme	essential	531:539	arg1	mechanisms					440:449	the mechanisms	436:449	the mechanisms involved	436:458	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	1	27	theme	obesity	189:195	arg1	prevalence					175:184	the prevalence	171:184	the prevalence of obesity	171:195	While the prevalence of obesity progresses worldwide, the consumption of sugars and dietary fiber increases and decreases, respectively.
37087279	11	28	theme	food	1692:1695	arg1	supplementation					1673:1687	the supplementation	1669:1687	the supplementation of food with healthy ingredients in the context of the current obesity epidemic	1669:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	2	29	theme	soluble	329:335	arg1	ingredient					365:374	a plant-based food ingredient	346:374	a plant-based food ingredient with beneficial effects in Humans	346:408	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	2	29	theme	soluble	329:335	arg1	fiber					337:341	NUTRIOSE® soluble fiber	319:341	NUTRIOSE® soluble fiber	319:341	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	4	30	theme	IGN	678:680	arg1	activation					664:673	the activation	660:673	the activation of IGN	660:680	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	7	31	theme	body	1088:1091	arg1	gain					1100:1103	body weight gain	1088:1103	body weight gain	1088:1103	Indeed, WT mice are protected from body weight gain and NAFLD induced by a high calorie diet.
37087279	9	32	from	itself	1378:1383	arg1	sufficient					1364:1373	sufficient	1364:1373	sufficient	1364:1373	While the gut microbiota composition changes with NUTRIOSE®, this is not sufficient in itself to account for the benefits observed.
37087279	2	33	theme	NUTRIOSE®	319:327	arg1	ingredient					365:374	a plant-based food ingredient	346:374	a plant-based food ingredient with beneficial effects in Humans	346:408	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	2	33	theme	NUTRIOSE®	319:327	arg1	fiber					337:341	NUTRIOSE® soluble fiber	319:341	NUTRIOSE® soluble fiber	319:341	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	6	34	theme	metabolic	991:999	arg1	benefits					1001:1008	metabolic benefits	991:1008	metabolic benefits	991:1008	In line with our hypothesis, NUTRIOSE® exerts metabolic benefits in WT mice, but not in IGN-deficient mice.
37087279	0	35	theme	metabolism	22:31	arg1	Improvement					0:10	Improvement	0:10	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties,	0:126	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	8	36	theme	adipose	1275:1281	arg1	tissue					1283:1288	subcutaneous white adipose tissue	1256:1288	subcutaneous white adipose tissue	1256:1288	In addition, our data suggests that NUTRIOSE® may improve energy balance by activating a browning process in subcutaneous white adipose tissue.
37087279	11	37	from	ingredients	1710:1720	arg1	context					1729:1735	the context	1725:1735	the context of the current obesity epidemic	1725:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	4	38	theme	beneficial	637:646	arg1	effects					648:654	its beneficial effects	633:654	its beneficial effects	633:654	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	0	39	theme	energy	15:20	arg1	metabolism					22:31	energy metabolism	15:31	energy metabolism	15:31	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	11	40	theme	NUTRIOSE®	1629:1637	arg1	effects					1618:1624	the beneficial effects	1603:1624	the beneficial effects of NUTRIOSE®	1603:1637	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	3	41	theme	beneficial	557:566	arg1	effects					568:574	the beneficial effects	553:574	the beneficial effects of dietary fibers	553:592	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	8	42	theme	energy	1205:1210	arg1	balance					1212:1218	energy balance	1205:1218	energy balance	1205:1218	In addition, our data suggests that NUTRIOSE® may improve energy balance by activating a browning process in subcutaneous white adipose tissue.
37087279	7	43	theme	WT	1061:1062	arg1	mice					1064:1067	WT mice	1061:1067	WT mice	1061:1067	Indeed, WT mice are protected from body weight gain and NAFLD induced by a high calorie diet.
37087279	11	44	theme	healthy	1702:1708	arg1	ingredients					1710:1720	healthy ingredients	1702:1720	healthy ingredients in the context of the current obesity epidemic	1702:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	6	45	with	line	948:951	arg1	hypothesis					962:971	our hypothesis	958:971	our hypothesis	958:971	In line with our hypothesis, NUTRIOSE® exerts metabolic benefits in WT mice, but not in IGN-deficient mice.
37087279	11	46	theme	epidemic	1760:1767	arg1	obesity					1752:1758	the current obesity	1740:1758	the current obesity epidemic	1740:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	11	47	theme	current	1744:1750	arg1	obesity					1752:1758	the current obesity	1740:1758	the current obesity epidemic	1740:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	0	48	theme	intestinal	137:146	arg1	gluconeogenesis					148:162	intestinal gluconeogenesis	137:162	intestinal gluconeogenesis	137:162	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	10	49	theme	NUTRIOSE®	1465:1473	arg1	benefits					1475:1482	the NUTRIOSE® benefits	1461:1482	the NUTRIOSE® benefits	1461:1482	On the contrary, IGN is obligatory in the NUTRIOSE® benefits, since no benefit take place in absence of IGN.
37087279	9	50	from	sufficient	1364:1373	arg1	itself					1378:1383	itself	1378:1383	itself	1378:1383	While the gut microbiota composition changes with NUTRIOSE®, this is not sufficient in itself to account for the benefits observed.
37087279	10	51	theme	IGN	1527:1529	arg1	absence					1516:1522	absence	1516:1522	absence of IGN	1516:1529	On the contrary, IGN is obligatory in the NUTRIOSE® benefits, since no benefit take place in absence of IGN.
37087279	0	52	theme	soluble	59:65	arg1	fiber					67:71	NUTRIOSE® soluble fiber	49:71	NUTRIOSE® soluble fiber	49:71	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	0	52	theme	soluble	59:65	arg1	ingredient					84:93	a dietary ingredient	74:93	a dietary ingredient exhibiting prebiotic properties	74:125	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	11	53	theme	supplementation	1673:1687	arg1	interest					1657:1664	the interest	1653:1664	the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic	1653:1767	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	3	54	theme	dietary	579:585	arg1	fibers					587:592	dietary fibers	579:592	dietary fibers	579:592	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	2	55	from	effects	392:398	arg1	Humans					403:408	Humans	403:408	Humans	403:408	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	2	56	theme	plant-based	348:358	arg1	ingredient					365:374	a plant-based food ingredient	346:374	a plant-based food ingredient with beneficial effects in Humans	346:408	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	2	56	theme	plant-based	348:358	arg1	fiber					337:341	NUTRIOSE® soluble fiber	319:341	NUTRIOSE® soluble fiber	319:341	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	0	57	theme	NUTRIOSE®	49:57	arg1	fiber					67:71	NUTRIOSE® soluble fiber	49:71	NUTRIOSE® soluble fiber	49:71	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	0	57	theme	NUTRIOSE®	49:57	arg1	ingredient					84:93	a dietary ingredient	74:93	a dietary ingredient exhibiting prebiotic properties	74:125	Improvement of energy metabolism associated with NUTRIOSE® soluble fiber, a dietary ingredient exhibiting prebiotic properties, requires intestinal gluconeogenesis.
37087279	11	58	theme	beneficial	1607:1616	arg1	effects					1618:1624	the beneficial effects	1603:1624	the beneficial effects of NUTRIOSE®	1603:1637	In conclusion, IGN plays a crucial and essential role in the set-up of the beneficial effects of NUTRIOSE®, highlighting the interest of the supplementation of food with healthy ingredients in the context of the current obesity epidemic.
37087279	2	59	with	ingredient	365:374	arg1	effects					392:398	beneficial effects	381:398	beneficial effects in Humans	381:408	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	3	60	theme	fibers	587:592	arg1	effects					568:574	the beneficial effects	553:574	the beneficial effects of dietary fibers	553:592	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	3	61	theme	gluconeogenesis	504:518	arg1	IGN					521:523	IGN	521:523	IGN	521:523	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	3	61	theme	gluconeogenesis	504:518	arg1	involvement					478:488	the involvement	474:488	the involvement of intestinal gluconeogenesis (IGN)	474:524	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	3	61	theme	gluconeogenesis	504:518	arg1	mechanisms					440:449	the mechanisms	436:449	the mechanisms involved	436:458	Here, we studied in mice the mechanisms involved, particularly the involvement of intestinal gluconeogenesis (IGN), the essential function in the beneficial effects of dietary fibers.
37087279	4	62	theme	fatty	785:789	arg1	NAFLD					806:810	NAFLD	806:810	NAFLD	806:810	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	4	62	theme	fatty	785:789	arg1	disease					797:803	non-alcoholic fatty liver disease	771:803	non-alcoholic fatty liver disease (NAFLD)	771:811	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	4	63	theme	NUTRIOSE®	717:725	arg1	effects					698:704	the effects	694:704	the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent	694:841	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	1	64	theme	sugars	238:243	arg1	consumption					223:233	the consumption	219:233	the consumption of sugars and dietary fiber	219:261	While the prevalence of obesity progresses worldwide, the consumption of sugars and dietary fiber increases and decreases, respectively.
37087279	4	65	theme	diabetes	758:765	arg1	development					734:744	the development	730:744	the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent	730:841	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	5	66	theme	IGN	866:868	arg1	role					858:861	the role	854:861	the role of IGN in the observed effects	854:892	To assert the role of IGN in the observed effects, we studied wild-type (WT) and IGN-deficient mice.
37087279	4	67	theme	non-alcoholic	771:783	arg1	NAFLD					806:810	NAFLD	806:810	NAFLD	806:810	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	4	67	theme	non-alcoholic	771:783	arg1	disease					797:803	non-alcoholic fatty liver disease	771:803	non-alcoholic fatty liver disease (NAFLD)	771:811	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	4	68	theme	dietary	709:715	arg1	NUTRIOSE®					717:725	dietary NUTRIOSE®	709:725	dietary NUTRIOSE®	709:725	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	5	69	dep	wild-type	906:914	arg1	WT					917:918	WT	917:918	WT	917:918	To assert the role of IGN in the observed effects, we studied wild-type (WT) and IGN-deficient mice.
37087279	10	70	from	obligatory	1447:1456	arg1	benefits					1475:1482	the NUTRIOSE® benefits	1461:1482	the NUTRIOSE® benefits	1461:1482	On the contrary, IGN is obligatory in the NUTRIOSE® benefits, since no benefit take place in absence of IGN.
37087279	4	71	theme	disease	797:803	arg1	development					734:744	the development	730:744	the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent	730:841	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	2	72	theme	beneficial	381:390	arg1	effects					392:398	beneficial effects	381:398	beneficial effects in Humans	381:408	In this context, NUTRIOSE® soluble fiber is a plant-based food ingredient with beneficial effects in Humans.
37087279	4	73	from	effects	698:704	arg1	development					734:744	the development	730:744	the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent	730:841	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	1	74	theme	dietary	249:255	arg1	fiber					257:261	dietary fiber	249:261	dietary fiber	249:261	While the prevalence of obesity progresses worldwide, the consumption of sugars and dietary fiber increases and decreases, respectively.
37087279	7	75	theme	high	1128:1131	arg1	diet					1141:1144	a high calorie diet	1126:1144	a high calorie diet	1126:1144	Indeed, WT mice are protected from body weight gain and NAFLD induced by a high calorie diet.
37087279	4	76	theme	liver	791:795	arg1	NAFLD					806:810	NAFLD	806:810	NAFLD	806:810	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	4	76	theme	liver	791:795	arg1	disease					797:803	non-alcoholic fatty liver disease	771:803	non-alcoholic fatty liver disease (NAFLD)	771:811	To determine whether NUTRIOSE® exerts its beneficial effects via the activation of IGN, we studied the effects of dietary NUTRIOSE® on the development of obesity, diabetes and non-alcoholic fatty liver disease (NAFLD), which IGN is able to prevent.
37087279	1	77	theme	fiber	257:261	arg1	consumption					223:233	the consumption	219:233	the consumption of sugars and dietary fiber	219:261	While the prevalence of obesity progresses worldwide, the consumption of sugars and dietary fiber increases and decreases, respectively.
35359187	8	0	from	staining	1206:1213	arg1	disc					1235:1238	the TMJ disc	1227:1238	the TMJ disc	1227:1238	Finally, Safranin-O staining for GAGs in the TMJ disc was localized to the intermediate zone in the sheep but was absent from the porcine disc.
35359187	2	1	from	sheep	409:413	arg1	METHODS					385:391	METHODS	385:391	METHODS	385:391	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	2	1	from	sheep	409:413	arg1	MATERIALS					371:379	MATERIALS	371:379	MATERIALS	371:379	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	1	2	from	use	314:316	arg1	development					325:335	the development	321:335	the development of tissue engineering strategies	321:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	1	3	theme	joint	266:270	arg1	organization					228:239	the structural and ultrastructural organization	193:239	the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies	193:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	10	4	theme	loading	1570:1576	arg1	patterns					1578:1585	functional loading patterns	1559:1585	functional loading patterns	1559:1585	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	9	5	theme	μCT	1412:1414	arg1	variables					1416:1424	TMJ μCT variables	1408:1424	TMJ μCT variables	1408:1424	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	11	6	theme	human-analog	1899:1910	arg1	applications					1912:1923	human-analog applications	1899:1923	human-analog applications	1899:1923	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	8	7	theme	Safranin-O	1195:1204	arg1	staining					1206:1213	Safranin-O staining	1195:1213	Safranin-O staining for GAGs in the TMJ disc	1195:1238	Finally, Safranin-O staining for GAGs in the TMJ disc was localized to the intermediate zone in the sheep but was absent from the porcine disc.
35359187	7	8	theme	Elastin	1111:1117	arg1	staining					1119:1126	Elastin staining	1111:1126	Elastin staining	1111:1126	Elastin staining was nearly twofold greater in sheep than in the pig disc.
35359187	8	9	theme	intermediate	1261:1272	arg1	zone					1274:1277	the intermediate zone	1257:1277	the intermediate zone in the sheep	1257:1290	Finally, Safranin-O staining for GAGs in the TMJ disc was localized to the intermediate zone in the sheep but was absent from the porcine disc.
35359187	9	10	dep	pig	1394:1396	arg1	the					1390:1392	the	1390:1392	the	1390:1392	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	10	dep	pig	1394:1396	arg1	variables					1416:1424	TMJ μCT variables	1408:1424	TMJ μCT variables	1408:1424	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	4	11	from	condyle	818:824	arg1	species					834:840	both species	829:840	both species	829:840	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	9	12	theme	disc	1463:1466	arg1	histology					1430:1438	histology	1430:1438	histology	1430:1438	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	12	theme	disc	1463:1466	arg1	differences					1375:1385	some important differences	1360:1385	some important differences in the pig and sheep TMJ μCT variables	1360:1424	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	12	theme	disc	1463:1466	arg1	composition					1444:1454	composition	1444:1454	composition	1444:1454	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	8	13	theme	TMJ	1231:1233	arg1	disc					1235:1238	the TMJ disc	1227:1238	the TMJ disc	1227:1238	Finally, Safranin-O staining for GAGs in the TMJ disc was localized to the intermediate zone in the sheep but was absent from the porcine disc.
35359187	4	14	theme	μCT	710:712	arg1	analyses					714:721	RESULTS μCT analyses	702:721	RESULTS μCT analyses	702:721	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	9	15	theme	discal	1472:1477	arg1	attachment					1479:1488	discal attachment	1472:1488	discal attachment	1472:1488	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	10	16	dep	masticatory	1539:1549	arg1	patterns					1578:1585	functional loading patterns	1559:1585	functional loading patterns	1559:1585	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	3	17	theme	regional	627:634	arg1	distribution					636:647	regional distribution	627:647	regional distribution of collagen, elastin, and glycosaminoglycans (GAGs)	627:699	Histological examination was performed on the TMJ disc and its attachments to investigate regional distribution of collagen, elastin, and glycosaminoglycans (GAGs).
35359187	5	18	theme	Quantitative	908:919	arg1	morphometry					921:931	Quantitative morphometry	908:931	Quantitative morphometry of the trabecular condyle	908:957	Quantitative morphometry of the trabecular condyle reveals no statistical differences between the species.
35359187	0	19	theme	TMJ	65:67	arg1	characterization					27:42	Morphologic and histologic characterization	0:42	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.	0:127	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	2	20	from	pigs	419:422	arg1	METHODS					385:391	METHODS	385:391	METHODS	385:391	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	2	20	from	pigs	419:422	arg1	MATERIALS					371:379	MATERIALS	371:379	MATERIALS	371:379	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	8	21	theme	porcine	1316:1322	arg1	disc					1324:1327	the porcine disc	1312:1327	the porcine disc	1312:1327	Finally, Safranin-O staining for GAGs in the TMJ disc was localized to the intermediate zone in the sheep but was absent from the porcine disc.
35359187	11	22	theme	animal	1769:1774	arg1	RELEVANCE					1694:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	22	theme	animal	1769:1774	arg1	sheep					1743:1747	the sheep	1739:1747	the sheep	1739:1747	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	22	theme	animal	1769:1774	arg1	model					1776:1780	a suitable large animal model	1752:1780	a suitable large animal model for TMJ research such as regenerative strategies	1752:1829	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	0	23	theme	large	72:76	arg1	models					85:90	large animal models	72:90	large animal models for tissue engineering applications	72:126	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	1	24	theme	study	155:159	arg1	aim					143:145	The aim	139:145	The aim of this study	139:159	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	2	25	theme	bone	520:523	arg1	parameters					525:534	bone parameters	520:534	bone parameters	520:534	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	1	26	dep	OBJECTIVE	129:137	arg1	was					161:163	was	161:163	was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies	161:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	11	27	theme	suitable	1754:1761	arg1	RELEVANCE					1694:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	27	theme	suitable	1754:1761	arg1	sheep					1743:1747	the sheep	1739:1747	the sheep	1739:1747	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	27	theme	suitable	1754:1761	arg1	model					1776:1780	a suitable large animal model	1752:1780	a suitable large animal model for TMJ research such as regenerative strategies	1752:1829	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	1	28	theme	tissue	340:345	arg1	strategies					359:368	tissue engineering strategies	340:368	tissue engineering strategies	340:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	2	29	theme	Whole	393:397	arg1	TMJs					399:402	Whole TMJs	393:402	Whole TMJs	393:402	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	0	30	theme	tissue	96:101	arg1	applications					115:126	tissue engineering applications	96:126	tissue engineering applications	96:126	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	1	31	theme	strategies	359:368	arg1	development					325:335	the development	321:335	the development of tissue engineering strategies	321:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	5	32	theme	statistical	970:980	arg1	differences					982:992	no statistical differences	967:992	no statistical differences between the species	967:1012	Quantitative morphometry of the trabecular condyle reveals no statistical differences between the species.
35359187	4	33	theme	bone	742:745	arg1	BMD					764:766	BMD	764:766	BMD	764:766	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	4	33	theme	bone	742:745	arg1	density					755:761	higher bone mineral density	735:761	higher bone mineral density (BMD)	735:767	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	10	34	theme	animal	1643:1648	arg1	models					1650:1655	large animal models	1637:1655	large animal models towards human applications	1637:1682	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	0	35	theme	Morphologic	0:10	arg1	characterization					27:42	Morphologic and histologic characterization	0:42	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.	0:127	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	0	36	theme	histologic	16:25	arg1	characterization					27:42	Morphologic and histologic characterization	0:42	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.	0:127	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	5	37	theme	trabecular	940:949	arg1	condyle					951:957	the trabecular condyle	936:957	the trabecular condyle	936:957	Quantitative morphometry of the trabecular condyle reveals no statistical differences between the species.
35359187	1	38	theme	animal	296:301	arg1	models					303:308	two large animal models	286:308	two large animal models for use in the development of tissue engineering strategies	286:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	6	39	theme	structural	1046:1055	arg1	organization					1057:1068	similar structural organization	1038:1068	similar structural organization of collagen and elastin between species	1038:1108	Histology demonstrates similar structural organization of collagen and elastin between species.
35359187	9	40	from	composition	1444:1454	arg1	sheep					1402:1406	sheep	1402:1406	sheep	1402:1406	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	40	from	composition	1444:1454	arg1	pig					1394:1396	pig	1394:1396	pig	1394:1396	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	3	41	theme	glycosaminoglycans	675:692	arg1	distribution					636:647	regional distribution	627:647	regional distribution of collagen, elastin, and glycosaminoglycans (GAGs)	627:699	Histological examination was performed on the TMJ disc and its attachments to investigate regional distribution of collagen, elastin, and glycosaminoglycans (GAGs).
35359187	4	42	theme	temporal	776:783	arg1	fossa					785:789	the temporal fossa	772:789	the temporal fossa compared to the mandibular condyle in both species	772:840	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	1	43	theme	ultrastructural	212:226	arg1	organization					228:239	the structural and ultrastructural organization	193:239	the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies	193:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	9	44	from	histology	1430:1438	arg1	sheep					1402:1406	sheep	1402:1406	sheep	1402:1406	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	44	from	histology	1430:1438	arg1	pig					1394:1396	pig	1394:1396	pig	1394:1396	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	11	45	theme	pig	1728:1730	arg1	model					1732:1736	the established pig model	1712:1736	the established pig model	1712:1736	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	46	theme	appropriate	1883:1893	arg1	parameters					1872:1881	design parameters	1865:1881	design parameters appropriate for human-analog applications	1865:1923	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	8	47	from	disc	1324:1327	arg1	absent					1300:1305	absent	1300:1305	absent	1300:1305	Finally, Safranin-O staining for GAGs in the TMJ disc was localized to the intermediate zone in the sheep but was absent from the porcine disc.
35359187	11	48	theme	design	1865:1870	arg1	parameters					1872:1881	design parameters	1865:1881	design parameters appropriate for human-analog applications	1865:1923	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	1	49	theme	temporomandibular	248:264	arg1	joint					266:270	the temporomandibular joint	244:270	the temporomandibular joint (TMJ)	244:276	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	1	49	theme	temporomandibular	248:264	arg1	TMJ					273:275	TMJ	273:275	TMJ	273:275	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	3	50	theme	Histological	537:548	arg1	examination					550:560	Histological examination	537:560	Histological examination	537:560	Histological examination was performed on the TMJ disc and its attachments to investigate regional distribution of collagen, elastin, and glycosaminoglycans (GAGs).
35359187	9	51	theme	TMJ	1408:1410	arg1	variables					1416:1424	TMJ μCT variables	1408:1424	TMJ μCT variables	1408:1424	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	10	52	theme	functional	1559:1568	arg1	patterns					1578:1585	functional loading patterns	1559:1585	functional loading patterns	1559:1585	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	11	53	theme	CLINICAL	1685:1692	arg1	RELEVANCE					1694:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	53	theme	CLINICAL	1685:1692	arg1	sheep					1743:1747	the sheep	1739:1747	the sheep	1739:1747	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	53	theme	CLINICAL	1685:1692	arg1	model					1776:1780	a suitable large animal model	1752:1780	a suitable large animal model for TMJ research such as regenerative strategies	1752:1829	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	54	with	As	1704:1705	arg1	model					1732:1736	the established pig model	1712:1736	the established pig model	1712:1736	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	55	theme	TMJ	1786:1788	arg1	research					1790:1797	TMJ research	1786:1797	TMJ research such as regenerative strategies	1786:1829	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	1	56	theme	structural	197:206	arg1	organization					228:239	the structural and ultrastructural organization	193:239	the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies	193:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	2	57	theme	parameters	525:534	arg1	morphology					480:489	morphology	480:489	morphology	480:489	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	2	57	theme	parameters	525:534	arg1	analyses					508:515	quantitative analyses	495:515	quantitative analyses of bone parameters	495:534	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	3	58	theme	TMJ	583:585	arg1	disc					587:590	the TMJ disc	579:590	the TMJ disc	579:590	Histological examination was performed on the TMJ disc and its attachments to investigate regional distribution of collagen, elastin, and glycosaminoglycans (GAGs).
35359187	4	59	theme	RESULTS	702:708	arg1	analyses					714:721	RESULTS μCT analyses	702:721	RESULTS μCT analyses	702:721	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	11	60	theme	regenerative	1807:1818	arg1	strategies					1820:1829	regenerative strategies	1807:1829	regenerative strategies	1807:1829	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	61	with	model	1776:1780	arg1	considerations					1846:1859	specific considerations	1837:1859	specific considerations for design parameters appropriate for human-analog applications	1837:1923	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	9	62	theme	attachment	1479:1488	arg1	histology					1430:1438	histology	1430:1438	histology	1430:1438	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	62	theme	attachment	1479:1488	arg1	differences					1375:1385	some important differences	1360:1385	some important differences in the pig and sheep TMJ μCT variables	1360:1424	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	62	theme	attachment	1479:1488	arg1	composition					1444:1454	composition	1444:1454	composition	1444:1454	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	6	63	theme	similar	1038:1044	arg1	organization					1057:1068	similar structural organization	1038:1068	similar structural organization of collagen and elastin between species	1038:1108	Histology demonstrates similar structural organization of collagen and elastin between species.
35359187	11	64	dep	model	1776:1780	arg1	As					1704:1705	As	1704:1705	As	1704:1705	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	8	65	from	zone	1274:1277	arg1	sheep					1286:1290	the sheep	1282:1290	the sheep	1282:1290	Finally, Safranin-O staining for GAGs in the TMJ disc was localized to the intermediate zone in the sheep but was absent from the porcine disc.
35359187	9	66	dep	CONCLUSIONS	1330:1340	arg1	show					1355:1358	show	1355:1358	show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment	1355:1488	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	0	67	theme	animal	78:83	arg1	models					85:90	large animal models	72:90	large animal models for tissue engineering applications	72:126	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	11	68	theme	established	1716:1726	arg1	model					1732:1736	the established pig model	1712:1736	the established pig model	1712:1736	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	2	69	dep	MATERIALS	371:379	arg1	TMJs					399:402	Whole TMJs	393:402	Whole TMJs	393:402	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	5	70	theme	condyle	951:957	arg1	morphometry					921:931	Quantitative morphometry	908:931	Quantitative morphometry of the trabecular condyle	908:957	Quantitative morphometry of the trabecular condyle reveals no statistical differences between the species.
35359187	11	71	theme	large	1763:1767	arg1	RELEVANCE					1694:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE	1685:1702	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	71	theme	large	1763:1767	arg1	sheep					1743:1747	the sheep	1739:1747	the sheep	1739:1747	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	11	71	theme	large	1763:1767	arg1	model					1776:1780	a suitable large animal model	1752:1780	a suitable large animal model for TMJ research such as regenerative strategies	1752:1829	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35359187	0	72	theme	engineering	103:113	arg1	applications					115:126	tissue engineering applications	96:126	tissue engineering applications	96:126	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	1	73	theme	engineering	347:357	arg1	strategies					359:368	tissue engineering strategies	340:368	tissue engineering strategies	340:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	2	74	theme	quantitative	495:506	arg1	analyses					508:515	quantitative analyses	495:515	quantitative analyses of bone parameters	495:534	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	6	75	theme	elastin	1086:1092	arg1	organization					1057:1068	similar structural organization	1038:1068	similar structural organization of collagen and elastin between species	1038:1108	Histology demonstrates similar structural organization of collagen and elastin between species.
35359187	4	76	theme	higher	735:740	arg1	BMD					764:766	BMD	764:766	BMD	764:766	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	4	76	theme	higher	735:740	arg1	density					755:761	higher bone mineral density	735:761	higher bone mineral density (BMD)	735:767	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	10	77	theme	large	1637:1641	arg1	models					1650:1655	large animal models	1637:1655	large animal models towards human applications	1637:1682	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	7	78	theme	pig	1176:1178	arg1	disc					1180:1183	the pig disc	1172:1183	the pig disc	1172:1183	Elastin staining was nearly twofold greater in sheep than in the pig disc.
35359187	6	79	theme	collagen	1073:1080	arg1	organization					1057:1068	similar structural organization	1038:1068	similar structural organization of collagen and elastin between species	1038:1108	Histology demonstrates similar structural organization of collagen and elastin between species.
35359187	3	80	theme	collagen	652:659	arg1	distribution					636:647	regional distribution	627:647	regional distribution of collagen, elastin, and glycosaminoglycans (GAGs)	627:699	Histological examination was performed on the TMJ disc and its attachments to investigate regional distribution of collagen, elastin, and glycosaminoglycans (GAGs).
35359187	4	81	theme	mineral	747:753	arg1	BMD					764:766	BMD	764:766	BMD	764:766	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	4	81	theme	mineral	747:753	arg1	density					755:761	higher bone mineral density	735:761	higher bone mineral density (BMD)	735:767	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	0	82	theme	sheep	47:51	arg1	TMJ					65:67	sheep and porcine TMJ	47:67	sheep and porcine TMJ as large animal models for tissue engineering applications	47:126	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	1	83	theme	large	290:294	arg1	models					303:308	two large animal models	286:308	two large animal models for use in the development of tissue engineering strategies	286:368	OBJECTIVE The aim of this study was to compare and characterize the structural and ultrastructural organization of the temporomandibular joint (TMJ) between two large animal models for use in the development of tissue engineering strategies.
35359187	10	84	from	differences	1524:1534	arg1	masticatory					1539:1549	masticatory	1539:1549	masticatory	1539:1549	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	10	84	from	differences	1524:1534	arg1	TMJ					1555:1557	TMJ	1555:1557	TMJ	1555:1557	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	10	85	theme	human	1665:1669	arg1	applications					1671:1682	human applications	1665:1682	human applications	1665:1682	These disparities likely reflect differences in masticatory and TMJ functional loading patterns between the two species and provide insights into large animal models towards human applications.
35359187	0	86	theme	porcine	57:63	arg1	TMJ					65:67	sheep and porcine TMJ	47:67	sheep and porcine TMJ as large animal models for tissue engineering applications	47:126	Morphologic and histologic characterization of sheep and porcine TMJ as large animal models for tissue engineering applications.
35359187	2	87	theme	micro-computed	444:457	arg1	tomography					459:468	micro-computed tomography	444:468	micro-computed tomography (μCT)	444:474	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	2	87	theme	micro-computed	444:457	arg1	μCT					471:473	μCT	471:473	μCT	471:473	MATERIALS AND METHODS Whole TMJs from sheep and pigs were evaluated with micro-computed tomography (μCT) for morphology and quantitative analyses of bone parameters.
35359187	4	88	theme	mandibular	807:816	arg1	condyle					818:824	the mandibular condyle	803:824	the mandibular condyle in both species	803:840	RESULTS μCT analyses demonstrate higher bone mineral density (BMD) in the temporal fossa compared to the mandibular condyle in both species, with this variable being significantly higher in sheep than pig.
35359187	9	89	theme	important	1365:1373	arg1	differences					1375:1385	some important differences	1360:1385	some important differences in the pig and sheep TMJ μCT variables	1360:1424	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	90	from	differences	1375:1385	arg1	sheep					1402:1406	sheep	1402:1406	sheep	1402:1406	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	9	90	from	differences	1375:1385	arg1	pig					1394:1396	pig	1394:1396	pig	1394:1396	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	3	91	theme	elastin	662:668	arg1	distribution					636:647	regional distribution	627:647	regional distribution of collagen, elastin, and glycosaminoglycans (GAGs)	627:699	Histological examination was performed on the TMJ disc and its attachments to investigate regional distribution of collagen, elastin, and glycosaminoglycans (GAGs).
35359187	9	92	dep	disc	1463:1466	arg1	the					1459:1461	the	1459:1461	the	1459:1461	CONCLUSIONS Our findings show some important differences in the pig and sheep TMJ μCT variables and histology and composition of the disc and discal attachment.
35359187	11	93	theme	specific	1837:1844	arg1	considerations					1846:1859	specific considerations	1837:1859	specific considerations for design parameters appropriate for human-analog applications	1837:1923	CLINICAL RELEVANCE As with the established pig model, the sheep is a suitable large animal model for TMJ research such as regenerative strategies, with specific considerations for design parameters appropriate for human-analog applications.
35472739	13	0	theme	fat	1938:1940	arg1	yield					1942:1946	the abdominal fat yield	1924:1946	the abdominal fat yield	1924:1946	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	1	1	theme	growth	201:206	arg1	performance					208:218	growth performance	201:218	growth performance	201:218	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	7	2	theme	rice	1110:1113	arg1	groups					1115:1120	dietary paddy rice groups	1096:1120	dietary paddy rice groups	1096:1120	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	6	3	theme	feed	989:992	arg1	intake					994:999	average daily feed intake	975:999	average daily feed intake	975:999	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	3	4	theme	feed	462:465	arg1	inclusion					524:532	feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion	462:532	feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion	462:532	The geese were raised for 42 d on feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion.
35472739	7	5	theme	dietary	1096:1102	arg1	groups					1115:1120	dietary paddy rice groups	1096:1120	dietary paddy rice groups	1096:1120	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	6	6	theme	average	975:981	arg1	intake					994:999	average daily feed intake	975:999	average daily feed intake	975:999	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	11	7	theme	P	1611:1611	arg1	group					1604:1608	the control group	1592:1608	the control group (P < 0.05)	1592:1619	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	11	7	theme	P	1611:1611	arg1	<					1613:1613	P < 0.05	1611:1618	P < 0.05	1611:1618	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	9	8	theme	control	1322:1328	arg1	group					1330:1334	the control group	1318:1334	the control group	1318:1334	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	5	9	used	used	821:824	arg2	one					813:815	one	813:815	one	813:815	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	1	10	from	traits	229:234	arg1	geese					284:288	geese	284:288	geese	284:288	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	2	11	contain	containing	399:408	arg1	pens					394:397	6 pens	392:397	6 pens containing 10 goslings each	392:425	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	2	11	contain	containing	399:408	arg2	goslings					413:420	10 goslings	410:420	10 goslings each	410:425	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	11	12	theme	total	1551:1555	arg1	digestibility					1564:1576	higher total starch digestibility	1544:1576	higher total starch digestibility	1544:1576	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	7	13	theme	abdominal	1073:1081	arg1	yield					1087:1091	The abdominal fat yield	1069:1091	The abdominal fat yield in dietary paddy rice groups	1069:1120	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	7	13	theme	abdominal	1073:1081	arg1	higher					1126:1131	higher	1126:1131	higher	1126:1131	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	13	14	theme	geese	1804:1808	arg1	diets					1810:1814	geese diets	1804:1814	geese diets	1804:1814	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	11	15	theme	paddy	1516:1520	arg1	groups					1527:1532	the paddy rice groups	1512:1532	the paddy rice groups	1512:1532	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	4	16	theme	feed	551:554	arg1	intake					556:561	feed intake	551:561	feed intake	551:561	Body weight and feed intake per pen were recorded from the arrival of goslings to the end of the trial.
35472739	0	17	from	traits	61:66	arg1	geese					116:120	geese	116:120	geese	116:120	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	6	18	theme	body	962:965	arg1	weight					967:972	a higher final body weight	947:972	a higher final body weight	947:972	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	8	19	theme	breast	1244:1249	arg1	effects					1190:1196	no effects	1187:1196	no effects on the moisture, protein, and fat contents of breast and thigh muscle	1187:1266	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	5	20	theme	nutrient	839:846	arg1	utilization					848:858	nutrient utilization	839:858	nutrient utilization	839:858	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	6	21	theme	higher	949:954	arg1	weight					967:972	a higher final body weight	947:972	a higher final body weight	947:972	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	13	22	theme	breast	1858:1863	arg1	color					1872:1876	breast muscle color	1858:1876	breast muscle color	1858:1876	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	10	23	theme	P	1492:1492	arg1	geese					1485:1489	geese	1485:1489	geese (P < 0.05)	1485:1500	Dietary paddy rice decreased the bill score of geese (P < 0.05).
35472739	10	23	theme	P	1492:1492	arg1	<					1494:1494	P < 0.05	1492:1499	P < 0.05	1492:1499	Dietary paddy rice decreased the bill score of geese (P < 0.05).
35472739	13	24	theme	abdominal	1928:1936	arg1	yield					1942:1946	the abdominal fat yield	1924:1946	the abdominal fat yield	1924:1946	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	0	25	from	color	79:83	arg1	geese					116:120	geese	116:120	geese	116:120	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	3	26	with	feed	462:465	arg1	control					476:482	control	476:482	control	476:482	The geese were raised for 42 d on feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion.
35472739	3	26	with	feed	462:465	arg1	%					473:473	0%	472:473	0% (control)	472:483	The geese were raised for 42 d on feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion.
35472739	6	27	theme	average	1006:1012	arg1	gain					1020:1023	average daily gain	1006:1023	average daily gain	1006:1023	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	5	28	used	used	707:710	arg2	pen					685:687	each pen	680:687	each pen	680:687	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	5	28	used	used	707:710	arg2	one					690:692	one	690:692	one	690:692	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	3	29	dep	feed	462:465	arg1	dietary					505:511	dietary	505:511	dietary	505:511	The geese were raised for 42 d on feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion.
35472739	7	30	from	yield	1087:1091	arg1	groups					1115:1120	dietary paddy rice groups	1096:1120	dietary paddy rice groups	1096:1120	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	6	31	theme	paddy	932:936	arg1	rice					938:941	26, 39, and 52% paddy rice	916:941	26, 39, and 52% paddy rice	916:941	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	4	32	theme	Body	535:538	arg1	weight					540:545	Body weight	535:545	Body weight	535:545	Body weight and feed intake per pen were recorded from the arrival of goslings to the end of the trial.
35472739	11	33	from	geese	1583:1587	arg1	group					1604:1608	the control group	1592:1608	the control group (P < 0.05)	1592:1619	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	11	33	from	geese	1583:1587	arg1	<					1613:1613	P < 0.05	1611:1618	P < 0.05	1611:1618	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	1	34	theme	nutrient	258:265	arg1	digestibility					267:279	nutrient digestibility	258:279	nutrient digestibility in geese	258:288	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	13	35	theme	geese	1979:1983	arg1	color					1970:1974	the bill color	1961:1974	the bill color of geese	1961:1983	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	9	36	dep	fed	1364:1366	arg1	had					1383:1385	had	1383:1385	fed the paddy rice had a lower L* value and a higher a* value (P < 0.05)	1364:1435	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	1	37	from	color	247:251	arg1	geese					284:288	geese	284:288	geese	284:288	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	9	38	theme	geese	1358:1362	arg1	muscle					1348:1353	the breast muscle	1337:1353	the breast muscle of geese	1337:1362	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	0	39	from	Effects	0:6	arg1	digestibility					99:111	nutrient digestibility	90:111	nutrient digestibility in geese	90:120	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	0	39	from	Effects	0:6	arg1	traits					61:66	carcass traits	53:66	carcass traits	53:66	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	0	39	from	Effects	0:6	arg1	color					79:83	bare skin color	69:83	bare skin color	69:83	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	0	39	from	Effects	0:6	arg1	performance					40:50	growth performance	33:50	growth performance	33:50	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	12	40	theme	fiber	1683:1687	arg1	digestibility					1626:1638	The digestibility	1622:1638	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF	1622:1701	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	0	41	theme	bare	69:72	arg1	color					79:83	bare skin color	69:83	bare skin color	69:83	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	12	42	theme	NDF	1690:1692	arg1	digestibility					1626:1638	The digestibility	1622:1638	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF	1622:1701	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	2	43	theme	goslings	332:339	arg1	total					293:297	A total	291:297	A total of 300 twenty-eight-day-old male goslings	291:339	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	5	44	theme	meat	760:763	arg1	composition					775:785	meat proximate composition	760:785	meat proximate composition	760:785	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	12	45	theme	ADF	1699:1701	arg1	digestibility					1626:1638	The digestibility	1622:1638	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF	1622:1701	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	12	46	theme	crude	1666:1670	arg1	fat					1672:1674	crude fat	1666:1674	crude fat	1666:1674	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	2	47	theme	twenty-eight-day-old	306:325	arg1	goslings					332:339	300 twenty-eight-day-old male goslings	302:339	300 twenty-eight-day-old male goslings	302:339	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	1	48	theme	carcass	221:227	arg1	traits					229:234	carcass traits	221:234	carcass traits	221:234	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	5	49	theme	slaughter	723:731	arg1	performance					733:743	slaughter performance	723:743	slaughter performance	723:743	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	2	50	theme	treatment	370:378	arg1	groups					380:385	5 treatment groups	368:385	5 treatment groups with 6 pens containing 10 goslings each	368:425	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	13	51	from	source	1794:1799	arg1	diets					1810:1814	geese diets	1804:1814	geese diets	1804:1814	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	13	51	from	source	1794:1799	arg1	conclusion					1748:1757	conclusion	1748:1757	conclusion	1748:1757	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	0	52	theme	dietary	11:17	arg1	rice					25:28	dietary paddy rice	11:28	dietary paddy rice	11:28	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	9	53	theme	L	1395:1395	arg1	value					1398:1402	a lower L* value	1387:1402	a lower L* value	1387:1402	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	0	54	theme	rice	25:28	arg1	Effects					0:6	Effects	0:6	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese	0:120	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	1	55	theme	skin	242:245	arg1	color					247:251	bare skin color	237:251	bare skin color	237:251	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	5	56	theme	skin	797:800	arg1	color					802:806	bare skin color	792:806	bare skin color	792:806	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	8	57	theme	moisture	1205:1212	arg1	contents					1232:1239	the moisture, protein, and fat contents	1201:1239	the moisture, protein, and fat contents	1201:1239	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	12	58	theme	protein	1657:1663	arg1	digestibility					1626:1638	The digestibility	1622:1638	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF	1622:1701	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	1	59	from	digestibility	267:279	arg1	geese					284:288	geese	284:288	geese	284:288	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	2	60	dep	goslings	413:420	arg1	each					422:425	each	422:425	each	422:425	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	13	61	theme	total	1898:1902	arg1	starch					1904:1909	total starch	1898:1909	total starch	1898:1909	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	8	62	theme	protein	1215:1221	arg1	contents					1232:1239	the moisture, protein, and fat contents	1201:1239	the moisture, protein, and fat contents	1201:1239	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	12	63	theme	energy	1643:1648	arg1	digestibility					1626:1638	The digestibility	1622:1638	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF	1622:1701	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	9	64	theme	a	1417:1417	arg1	value					1420:1424	a higher a* value	1408:1424	a higher a* value (P < 0.05)	1408:1435	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	9	64	theme	a	1417:1417	arg1	<					1429:1429	P < 0.05	1427:1434	P < 0.05	1427:1434	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	0	65	from	digestibility	99:111	arg1	geese					116:120	geese	116:120	geese	116:120	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	3	66	theme	paddy	513:517	arg1	inclusion					524:532	feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion	462:532	feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion	462:532	The geese were raised for 42 d on feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion.
35472739	13	67	theme	muscle	1865:1870	arg1	color					1872:1876	breast muscle color	1858:1876	breast muscle color	1858:1876	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	1	68	theme	paddy	187:191	arg1	rice					193:196	dietary paddy rice	179:196	dietary paddy rice	179:196	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	7	69	theme	control	1150:1156	arg1	<					1167:1167	P < 0.05	1165:1172	P < 0.05	1165:1172	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	7	69	theme	control	1150:1156	arg1	group					1158:1162	the control group	1146:1162	the control group (P < 0.05)	1146:1173	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	9	70	theme	P	1427:1427	arg1	value					1420:1424	a higher a* value	1408:1424	a higher a* value (P < 0.05)	1408:1435	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	9	70	theme	P	1427:1427	arg1	<					1429:1429	P < 0.05	1427:1434	P < 0.05	1427:1434	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	6	71	contain	containing	905:914	arg1	diet					900:903	a diet	898:903	a diet containing 26, 39, and 52% paddy rice	898:941	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	71	contain	containing	905:914	arg2	rice					938:941	26, 39, and 52% paddy rice	916:941	26, 39, and 52% paddy rice	916:941	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	8	72	from	effects	1190:1196	arg1	contents					1232:1239	the moisture, protein, and fat contents	1201:1239	the moisture, protein, and fat contents	1201:1239	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	11	73	theme	control	1596:1602	arg1	group					1604:1608	the control group	1592:1608	the control group (P < 0.05)	1592:1619	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	11	73	theme	control	1596:1602	arg1	<					1613:1613	P < 0.05	1611:1618	P < 0.05	1611:1618	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	8	74	theme	thigh	1255:1259	arg1	effects					1190:1196	no effects	1187:1196	no effects on the moisture, protein, and fat contents of breast and thigh muscle	1187:1266	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	6	75	theme	daily	983:987	arg1	intake					994:999	average daily feed intake	975:999	average daily feed intake	975:999	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	0	76	theme	growth	33:38	arg1	performance					40:50	growth performance	33:50	growth performance	33:50	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	7	77	theme	paddy	1104:1108	arg1	groups					1115:1120	dietary paddy rice groups	1096:1120	dietary paddy rice groups	1096:1120	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	13	78	theme	starch	1904:1909	arg1	performance					1845:1855	the growth performance	1834:1855	the growth performance	1834:1855	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	13	78	theme	starch	1904:1909	arg1	color					1872:1876	breast muscle color	1858:1876	breast muscle color	1858:1876	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	13	78	theme	starch	1904:1909	arg1	utilization					1883:1893	utilization	1883:1893	utilization of total starch	1883:1909	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	10	79	theme	geese	1485:1489	arg1	score					1476:1480	the bill score	1467:1480	the bill score of geese (P < 0.05)	1467:1500	Dietary paddy rice decreased the bill score of geese (P < 0.05).
35472739	11	80	theme	rice	1522:1525	arg1	groups					1527:1532	the paddy rice groups	1512:1532	the paddy rice groups	1512:1532	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	1	81	theme	study	139:143	arg1	aim					127:129	The aim	123:129	The aim of this study	123:143	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	6	82	theme	control	1043:1049	arg1	group					1051:1055	the control group	1039:1055	the control group (P < 0.05)	1039:1066	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	82	theme	control	1043:1049	arg1	<					1060:1060	P < 0.05	1058:1065	P < 0.05	1058:1065	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	11	83	theme	starch	1557:1562	arg1	digestibility					1564:1576	higher total starch digestibility	1544:1576	higher total starch digestibility	1544:1576	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	7	84	theme	fat	1083:1085	arg1	yield					1087:1091	The abdominal fat yield	1069:1091	The abdominal fat yield in dietary paddy rice groups	1069:1120	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	7	84	theme	fat	1083:1085	arg1	higher					1126:1131	higher	1126:1131	higher	1126:1131	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	10	85	theme	bill	1471:1474	arg1	score					1476:1480	the bill score	1467:1480	the bill score of geese (P < 0.05)	1467:1500	Dietary paddy rice decreased the bill score of geese (P < 0.05).
35472739	11	86	theme	higher	1544:1549	arg1	digestibility					1564:1576	higher total starch digestibility	1544:1576	higher total starch digestibility	1544:1576	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	6	87	theme	final	956:960	arg1	weight					967:972	a higher final body weight	947:972	a higher final body weight	947:972	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	88	contain	had	943:945	arg1	goslings					885:892	goslings	885:892	goslings fed a diet containing 26, 39, and 52% paddy rice	885:941	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	88	contain	had	943:945	arg2	intake					994:999	average daily feed intake	975:999	average daily feed intake	975:999	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	88	contain	had	943:945	arg2	weight					967:972	a higher final body weight	947:972	a higher final body weight	947:972	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	88	contain	had	943:945	arg2	gain					1020:1023	average daily gain	1006:1023	average daily gain	1006:1023	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	1	89	from	effects	168:174	arg1	color					247:251	bare skin color	237:251	bare skin color	237:251	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	1	89	from	effects	168:174	arg1	performance					208:218	growth performance	201:218	growth performance	201:218	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	1	89	from	effects	168:174	arg1	traits					229:234	carcass traits	221:234	carcass traits	221:234	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	1	89	from	effects	168:174	arg1	digestibility					267:279	nutrient digestibility	258:279	nutrient digestibility in geese	258:288	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	1	90	from	performance	208:218	arg1	geese					284:288	geese	284:288	geese	284:288	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	0	91	theme	nutrient	90:97	arg1	digestibility					99:111	nutrient digestibility	90:111	nutrient digestibility in geese	90:120	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	12	92	theme	P	1734:1734	arg1	>					1736:1736	P > 0.05	1734:1741	P > 0.05	1734:1741	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	12	92	theme	P	1734:1734	arg1	groups					1726:1731	groups	1726:1731	groups (P > 0.05)	1726:1742	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	6	93	dep	%	930:930	arg1	39					920:921	39	920:921	39	920:921	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	93	dep	%	930:930	arg1	52					928:929	52	928:929	52	928:929	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	94	theme	daily	1014:1018	arg1	gain					1020:1023	average daily gain	1006:1023	average daily gain	1006:1023	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	2	95	with	groups	380:385	arg1	pens					394:397	6 pens	392:397	6 pens containing 10 goslings each	392:425	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	6	96	dep	rice	938:941	arg1	%					930:930	%	930:930	%	930:930	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	0	97	theme	carcass	53:59	arg1	traits					61:66	carcass traits	53:66	carcass traits	53:66	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	5	98	theme	pen	685:687	arg1	one					690:692	one	690:692	one	690:692	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	5	98	theme	pen	685:687	arg1	pen					685:687	each pen	680:687	each pen	680:687	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	9	99	theme	breast	1341:1346	arg1	muscle					1348:1353	the breast muscle	1337:1353	the breast muscle of geese	1337:1362	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	6	100	theme	P	1058:1058	arg1	group					1051:1055	the control group	1039:1055	the control group (P < 0.05)	1039:1066	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	6	100	theme	P	1058:1058	arg1	<					1060:1060	P < 0.05	1058:1065	P < 0.05	1058:1065	The results showed that goslings fed a diet containing 26, 39, and 52% paddy rice had a higher final body weight, average daily feed intake, and average daily gain than those in the control group (P < 0.05).
35472739	0	101	theme	skin	74:77	arg1	color					79:83	bare skin color	69:83	bare skin color	69:83	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	8	102	dep	breast	1244:1249	arg1	muscle					1261:1266	muscle	1261:1266	muscle	1261:1266	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	5	103	theme	meat	746:749	arg1	quality					751:757	meat quality	746:757	meat quality	746:757	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	0	104	from	performance	40:50	arg1	geese					116:120	geese	116:120	geese	116:120	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	12	105	theme	crude	1677:1681	arg1	fiber					1683:1687	crude fiber	1677:1687	crude fiber	1677:1687	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	9	106	theme	paddy	1372:1376	arg1	rice					1378:1381	the paddy rice	1368:1381	the paddy rice	1368:1381	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	4	107	theme	goslings	605:612	arg1	arrival					594:600	the arrival	590:600	the arrival of goslings to the end of the trial	590:636	Body weight and feed intake per pen were recorded from the arrival of goslings to the end of the trial.
35472739	2	108	theme	male	327:330	arg1	goslings					332:339	300 twenty-eight-day-old male goslings	302:339	300 twenty-eight-day-old male goslings	302:339	A total of 300 twenty-eight-day-old male goslings were randomly divided into 5 treatment groups with 6 pens containing 10 goslings each.
35472739	13	109	theme	bill	1965:1968	arg1	color					1970:1974	the bill color	1961:1974	the bill color of geese	1961:1983	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	11	110	from	Geese	1503:1507	arg1	groups					1527:1532	the paddy rice groups	1512:1532	the paddy rice groups	1512:1532	Geese in the paddy rice groups exhibited higher total starch digestibility than geese in the control group (P < 0.05).
35472739	0	111	theme	paddy	19:23	arg1	rice					25:28	dietary paddy rice	11:28	dietary paddy rice	11:28	Effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	9	112	theme	lower	1389:1393	arg1	value					1398:1402	a lower L* value	1387:1402	a lower L* value	1387:1402	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	12	113	theme	fat	1672:1674	arg1	digestibility					1626:1638	The digestibility	1622:1638	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF	1622:1701	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	1	114	theme	bare	237:240	arg1	color					247:251	bare skin color	237:251	bare skin color	237:251	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	5	115	theme	bare	792:795	arg1	color					802:806	bare skin color	792:806	bare skin color	792:806	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	9	116	theme	*	1396:1396	arg1	value					1398:1402	a lower L* value	1387:1402	a lower L* value	1387:1402	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	13	117	theme	excellent	1777:1785	arg1	paddy					1760:1764	paddy	1760:1764	paddy	1760:1764	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	13	117	theme	excellent	1777:1785	arg1	source					1794:1799	an excellent energy source	1774:1799	an excellent energy source	1774:1799	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	4	118	theme	trial	632:636	arg1	end					621:623	the end	617:623	the end of the trial	617:636	Body weight and feed intake per pen were recorded from the arrival of goslings to the end of the trial.
35472739	13	119	theme	energy	1787:1792	arg1	paddy					1760:1764	paddy	1760:1764	paddy	1760:1764	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	13	119	theme	energy	1787:1792	arg1	source					1794:1799	an excellent energy source	1774:1799	an excellent energy source	1774:1799	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	3	120	theme	rice	519:522	arg1	inclusion					524:532	feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion	462:532	feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion	462:532	The geese were raised for 42 d on feed with 0% (control), 13, 26, 39, or 52% dietary paddy rice inclusion.
35472739	12	121	theme	crude	1651:1655	arg1	protein					1657:1663	crude protein	1651:1663	crude protein	1651:1663	The digestibility of energy, crude protein, crude fat, crude fiber, NDF, and ADF did not differ between groups (P > 0.05).
35472739	5	122	theme	proximate	765:773	arg1	composition					775:785	meat proximate composition	760:785	meat proximate composition	760:785	On d 70, two goslings were selected from each pen, one of which was used to measure slaughter performance, meat quality, meat proximate composition, and bare skin color, and one was used to determine nutrient utilization.
35472739	9	123	theme	higher	1410:1415	arg1	value					1420:1424	a higher a* value	1408:1424	a higher a* value (P < 0.05)	1408:1435	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	9	123	theme	higher	1410:1415	arg1	<					1429:1429	P < 0.05	1427:1434	P < 0.05	1427:1434	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	8	124	theme	fat	1228:1230	arg1	contents					1232:1239	the moisture, protein, and fat contents	1201:1239	the moisture, protein, and fat contents	1201:1239	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	13	125	theme	growth	1838:1843	arg1	performance					1845:1855	the growth performance	1834:1855	the growth performance	1834:1855	In conclusion, paddy rice is an excellent energy source in geese diets and could improve the growth performance, breast muscle color, and utilization of total starch but increase the abdominal fat yield and decrease the bill color of geese.
35472739	1	126	theme	dietary	179:185	arg1	rice					193:196	dietary paddy rice	179:196	dietary paddy rice	179:196	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
35472739	7	127	theme	P	1165:1165	arg1	<					1167:1167	P < 0.05	1165:1172	P < 0.05	1165:1172	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	7	127	theme	P	1165:1165	arg1	group					1158:1162	the control group	1146:1162	the control group (P < 0.05)	1146:1173	The abdominal fat yield in dietary paddy rice groups was higher than that of the control group (P < 0.05).
35472739	9	128	theme	*	1418:1418	arg1	value					1420:1424	a higher a* value	1408:1424	a higher a* value (P < 0.05)	1408:1435	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	9	128	theme	*	1418:1418	arg1	<					1429:1429	P < 0.05	1427:1434	P < 0.05	1427:1434	Compared to the control group, the breast muscle of geese fed the paddy rice had a lower L* value and a higher a* value (P < 0.05).
35472739	10	129	theme	Dietary	1438:1444	arg1	paddy					1446:1450	Dietary paddy	1438:1450	Dietary paddy	1438:1450	Dietary paddy rice decreased the bill score of geese (P < 0.05).
35472739	8	130	theme	P	1295:1295	arg1	treatments					1283:1292	the five treatments	1274:1292	the five treatments (P > 0.05)	1274:1303	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	8	130	theme	P	1295:1295	arg1	>					1297:1297	P > 0.05	1295:1302	P > 0.05	1295:1302	There were no effects on the moisture, protein, and fat contents of breast and thigh muscle among the five treatments (P > 0.05).
35472739	1	131	theme	rice	193:196	arg1	effects					168:174	the effects	164:174	the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese	164:288	The aim of this study was to investigate the effects of dietary paddy rice on growth performance, carcass traits, bare skin color, and nutrient digestibility in geese.
36537820	11	0	theme	human	2214:2218	arg1	ecosystem					2241:2249	the human intestinal microbial ecosystem	2210:2249	the human intestinal microbial ecosystem	2210:2249	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	9	1	theme	taxonomic	1971:1979	arg1	structure					1981:1989	the taxonomic structure	1967:1989	the taxonomic structure of the human gut microbiota	1967:2017	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	4	2	theme	Viable	593:598	arg1	counts					600:605	Viable counts	593:605	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB)	593:659	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	6	3	theme	farming	1164:1170	arg1	techniques					1172:1181	farming techniques	1164:1181	farming techniques	1164:1181	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	11	4	theme	diseases	2320:2327	arg1	onset					2282:2286	the onset	2278:2286	the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world	2278:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	2	5	theme	intestinal	352:361	arg1	structure					383:391	the intestinal microbial community structure	348:391	the intestinal microbial community structure	348:391	It was hypothesized that the consumption of raw vegetables could contribute to the maintenance of the intestinal microbial community structure.
36537820	9	6	theme	human	1998:2002	arg1	microbiota					2008:2017	the human gut microbiota	1994:2017	the human gut microbiota	1994:2017	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	10	7	theme	IMPORTANCE	2020:2029	arg1	diets					2044:2048	IMPORTANCE Western-type diets	2020:2048	IMPORTANCE Western-type diets	2020:2048	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	6	8	theme	conventional	1249:1260	arg1	salads					1270:1275	conventional farming salads	1249:1275	conventional farming salads	1249:1275	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	13	9	theme	microbes	2691:2698	arg1	salad					2581:2585	rocket salad	2574:2585	rocket salad (a widely consumed vegetal food frequently eaten raw)	2574:2639	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	13	9	theme	microbes	2691:2698	arg1	source					2650:2655	a source	2648:2655	a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota	2648:2810	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	11	10	theme	microbial	2231:2239	arg1	ecosystem					2241:2249	the human intestinal microbial ecosystem	2210:2249	the human intestinal microbial ecosystem	2210:2249	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	6	11	theme	rocket	1136:1141	arg1	samples					1143:1149	rocket samples	1136:1149	rocket samples according to farming techniques	1136:1181	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	9	12	theme	microbiota	2008:2017	arg1	structure					1981:1989	the taxonomic structure	1967:1989	the taxonomic structure of the human gut microbiota	1967:2017	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	1	13	theme	gastrointestinal	172:187	arg1	microbiota					189:198	the gastrointestinal microbiota	168:198	the gastrointestinal microbiota	168:198	Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases.
36537820	11	14	theme	microbe-depleted	2160:2175	arg1	diet					2177:2180	a microbe-depleted diet	2158:2180	a microbe-depleted diet	2158:2180	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	4	15	theme	bacteria	646:653	arg1	counts					600:605	Viable counts	593:605	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB)	593:659	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	13	16	theme	vegetal	2606:2612	arg1	food					2614:2617	a widely consumed vegetal food	2588:2617	a widely consumed vegetal food frequently eaten raw	2588:2638	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	8	17	dep	in	1611:1612	arg1	vitro					1614:1618	vitro	1614:1618	vitro	1614:1618	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	2	18	theme	raw	294:296	arg1	vegetables					298:307	raw vegetables	294:307	raw vegetables	294:307	It was hypothesized that the consumption of raw vegetables could contribute to the maintenance of the intestinal microbial community structure.
36537820	13	19	theme	intestinal	2790:2799	arg1	microbiota					2801:2810	the intestinal microbiota	2786:2810	the intestinal microbiota	2786:2810	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	11	20	theme	developed	2397:2405	arg1	world					2407:2411	the developed world	2393:2411	the developed world	2393:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	5	21	theme	traditional	1043:1053	arg1	farming					1055:1061	traditional farming	1043:1061	traditional farming	1043:1061	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	10	22	with	foods	2066:2070	arg1	microorganisms					2117:2130	naturally occurring microorganisms	2097:2130	naturally occurring microorganisms	2097:2130	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	10	22	with	foods	2066:2070	arg1	amount					2087:2092	a reduced amount	2077:2092	a reduced amount of naturally occurring microorganisms	2077:2130	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	6	23	theme	bacterial	1192:1200	arg1	taxa					1202:1205	several bacterial taxa	1184:1205	several bacterial taxa	1184:1205	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	4	24	theme	de	706:707	arg1	MRS					728:730	MRS	728:730	MRS	728:730	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	4	24	theme	de	706:707	arg1	Man-Rogosa-Sharpe					709:725	de Man-Rogosa-Sharpe	706:725	de Man-Rogosa-Sharpe (MRS)	706:731	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	10	25	theme	microorganisms	2117:2130	arg1	microorganisms					2117:2130	naturally occurring microorganisms	2097:2130	naturally occurring microorganisms	2097:2130	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	10	25	theme	microorganisms	2117:2130	arg1	amount					2087:2092	a reduced amount	2077:2092	a reduced amount of naturally occurring microorganisms	2077:2130	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	6	26	theme	farming	1294:1300	arg1	practices					1302:1310	farming practices	1294:1310	farming practices	1294:1310	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	4	27	theme	count	685:689	arg1	agar					691:694	plate count agar	679:694	plate count agar (PCA)	679:700	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	4	27	theme	count	685:689	arg1	PCA					697:699	PCA	697:699	PCA	697:699	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	12	28	theme	ready-to-eat	2486:2497	arg1	salads					2506:2511	commercial ready-to-eat rocket salads	2475:2511	commercial ready-to-eat rocket salads produced through different farming practices	2475:2556	Here, we studied the microorganisms that are associated with commercial ready-to-eat rocket salads produced through different farming practices.
36537820	9	29	theme	ready-to-eat	1829:1840	arg1	salads					1849:1854	commercial ready-to-eat rocket salads	1818:1854	commercial ready-to-eat rocket salads	1818:1854	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	0	30	theme	Human	70:74	arg1	Microbiome					76:85	the Human Microbiome	66:85	the Human Microbiome	66:85	Ready-To-Eat Rocket Salads as Potential Reservoir of Bacteria for the Human Microbiome.
36537820	1	31	theme	microbial	146:154	arg1	taxa					156:159	key microbial taxa	142:159	key microbial taxa within the gastrointestinal microbiota	142:198	Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases.
36537820	6	32	theme	communities	1371:1381	arg1	composition					1339:1349	the taxonomic composition	1325:1349	the taxonomic composition of rocket bacterial communities	1325:1381	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	4	33	dep	agar	691:694	arg1	agar					733:736	agar	733:736	agar	733:736	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	9	34	theme	live	1863:1866	arg1	bacteria					1868:1875	live bacteria	1863:1875	live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota	1863:2017	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	6	35	theme	rocket	1354:1359	arg1	communities					1371:1381	rocket bacterial communities	1354:1381	rocket bacterial communities	1354:1381	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	13	36	theme	rocket	2574:2579	arg1	salad					2581:2585	rocket salad	2574:2585	rocket salad (a widely consumed vegetal food frequently eaten raw)	2574:2639	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	13	36	theme	rocket	2574:2579	arg1	source					2650:2655	a source	2648:2655	a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota	2648:2810	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	0	37	theme	Ready-To-Eat	0:11	arg1	Rocket					13:18	Ready-To-Eat Rocket	0:18	Ready-To-Eat Rocket	0:18	Ready-To-Eat Rocket Salads as Potential Reservoir of Bacteria for the Human Microbiome.
36537820	5	38	theme	vertical	904:911	arg1	farming					913:919	vertical farming	904:919	vertical farming	904:919	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	14	39	theme	all	2855:2857	arg1	foods					2867:2871	virtually all vegetal foods	2845:2871	virtually all vegetal foods that are consumed raw	2845:2893	This deduction may be valid for virtually all vegetal foods that are consumed raw.
36537820	7	40	theme	farming	1557:1563	arg1	practices					1565:1573	farming practices	1557:1573	farming practices	1557:1573	LAB were isolated from only traditional farming samples and belonged to different species, which were variably distributed among samples and could be partly associated with farming practices.
36537820	1	41	theme	Western-type	100:111	arg1	diets					113:117	Western-type diets	100:117	Western-type diets	100:117	Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases.
36537820	11	42	theme	metabolic	2299:2307	arg1	diseases					2320:2327	chronic metabolic and immune diseases	2291:2327	chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world	2291:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	4	43	theme	rRNA	784:787	arg1	sequencing					794:803	16S rRNA gene sequencing	780:803	16S rRNA gene sequencing	780:803	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	3	44	theme	rocket	459:464	arg1	salads					466:471	commercial rocket salads	448:471	commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming	448:590	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	0	45	theme	Potential	30:38	arg1	Reservoir					40:48	Potential Reservoir	30:48	Potential Reservoir of Bacteria for the Human Microbiome	30:85	Ready-To-Eat Rocket Salads as Potential Reservoir of Bacteria for the Human Microbiome.
36537820	13	46	theme	acid	2667:2670	arg1	bacteria					2672:2679	lactic acid bacteria	2660:2679	lactic acid bacteria	2660:2679	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	9	47	theme	gastrointestinal	1913:1928	arg1	transit					1930:1936	gastrointestinal transit	1913:1936	gastrointestinal transit	1913:1936	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	8	48	theme	INFOGEST	1589:1596	arg1	protocol					1598:1605	the INFOGEST protocol	1585:1605	the INFOGEST protocol for in vitro simulation of gastrointestinal digestion	1585:1659	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	5	49	theme	different	1001:1009	arg1	structure					982:990	a bacterial community structure	960:990	a bacterial community structure markedly different from that of rocket salads from traditional farming	960:1061	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	13	50	theme	gastrointestinal	2717:2732	arg1	transit					2734:2740	gastrointestinal transit	2717:2740	gastrointestinal transit	2717:2740	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	6	51	theme	β-diversity	1093:1103	arg1	analyses					1105:1112	α- and β-diversity analyses	1086:1112	α- and β-diversity analyses	1086:1112	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	3	52	theme	different	490:498	arg1	practices					508:516	different farming practices	490:516	different farming practices	490:516	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	6	53	theme	α-	1086:1087	arg1	analyses					1105:1112	α- and β-diversity analyses	1086:1112	α- and β-diversity analyses	1086:1112	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	4	54	theme	mesophilic	610:619	arg1	bacteria					621:628	mesophilic bacteria	610:628	mesophilic bacteria	610:628	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	4	55	theme	lactic	634:639	arg1	LAB					656:658	LAB	656:658	LAB	656:658	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	4	55	theme	lactic	634:639	arg1	bacteria					646:653	lactic acid bacteria	634:653	lactic acid bacteria (LAB)	634:659	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	2	56	theme	community	373:381	arg1	structure					383:391	the intestinal microbial community structure	348:391	the intestinal microbial community structure	348:391	It was hypothesized that the consumption of raw vegetables could contribute to the maintenance of the intestinal microbial community structure.
36537820	8	57	theme	rocket-associated	1707:1723	arg1	bacteria					1725:1732	rocket-associated bacteria	1707:1732	several taxonomically different rocket-associated bacteria (particularly LAB)	1675:1751	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	1	58	theme	noncommunicable	224:238	arg1	diseases					240:247	noncommunicable diseases	224:247	noncommunicable diseases	224:247	Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases.
36537820	13	59	theme	bacteria	2672:2679	arg1	salad					2581:2585	rocket salad	2574:2585	rocket salad (a widely consumed vegetal food frequently eaten raw)	2574:2639	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	13	59	theme	bacteria	2672:2679	arg1	source					2650:2655	a source	2648:2655	a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota	2648:2810	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	5	60	theme	bacterial	962:970	arg1	structure					982:990	a bacterial community structure	960:990	a bacterial community structure markedly different from that of rocket salads from traditional farming	960:1061	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	12	61	theme	different	2530:2538	arg1	practices					2548:2556	different farming practices	2530:2556	different farming practices	2530:2556	Here, we studied the microorganisms that are associated with commercial ready-to-eat rocket salads produced through different farming practices.
36537820	11	62	theme	immune	2313:2318	arg1	diseases					2320:2327	chronic metabolic and immune diseases	2291:2327	chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world	2291:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	3	63	theme	traditional	519:529	arg1	practices					508:516	different farming practices	490:516	different farming practices	490:516	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	4	64	theme	total	825:829	arg1	bacteria					831:838	total bacteria	825:838	total bacteria associated with rocket salads	825:868	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	5	65	contain	had	956:958	arg2	structure					982:990	a bacterial community structure	960:990	a bacterial community structure markedly different from that of rocket salads from traditional farming	960:1061	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	5	65	contain	had	956:958	arg1	salads					892:897	rocket salads	885:897	rocket salads from vertical farming	885:919	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	8	66	theme	digestion	1651:1659	arg1	simulation					1620:1629	in vitro simulation	1611:1629	in vitro simulation of gastrointestinal digestion	1611:1659	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	11	67	theme	intestinal	2220:2229	arg1	ecosystem					2241:2249	the human intestinal microbial ecosystem	2210:2249	the human intestinal microbial ecosystem	2210:2249	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	7	68	theme	farming	1424:1430	arg1	samples					1432:1438	only traditional farming samples	1407:1438	only traditional farming samples	1407:1438	LAB were isolated from only traditional farming samples and belonged to different species, which were variably distributed among samples and could be partly associated with farming practices.
36537820	7	69	theme	different	1456:1464	arg1	species					1466:1472	different species	1456:1472	different species	1456:1472	LAB were isolated from only traditional farming samples and belonged to different species, which were variably distributed among samples and could be partly associated with farming practices.
36537820	11	70	theme	death	2384:2388	arg1	causes					2374:2379	the most significant causes	2353:2379	the most significant causes of death in the developed world	2353:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	12	71	theme	commercial	2475:2484	arg1	salads					2506:2511	commercial ready-to-eat rocket salads	2475:2511	commercial ready-to-eat rocket salads produced through different farming practices	2475:2556	Here, we studied the microorganisms that are associated with commercial ready-to-eat rocket salads produced through different farming practices.
36537820	10	72	theme	Western-type	2031:2042	arg1	diets					2044:2048	IMPORTANCE Western-type diets	2020:2048	IMPORTANCE Western-type diets	2020:2048	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	6	73	theme	farming	1262:1268	arg1	salads					1270:1275	conventional farming salads	1249:1275	conventional farming salads	1249:1275	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	1	74	theme	taxa	156:159	arg1	loss					134:137	the loss	130:137	the loss of key microbial taxa within the gastrointestinal microbiota	130:198	Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases.
36537820	9	75	theme	gut	2004:2006	arg1	microbiota					2008:2017	the human gut microbiota	1994:2017	the human gut microbiota	1994:2017	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	5	76	from	that	1016:1019	arg1	different					1001:1009	different	1001:1009	different	1001:1009	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	3	77	theme	vertical	575:582	arg1	farming					584:590	vertical farming	575:590	vertical farming	575:590	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	8	78	theme	gastrointestinal	1767:1782	arg1	transit					1784:1790	gastrointestinal transit	1767:1790	gastrointestinal transit	1767:1790	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	11	79	theme	ecosystem	2241:2249	arg1	alteration					2196:2205	the alteration	2192:2205	the alteration of the human intestinal microbial ecosystem	2192:2249	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	5	80	contain	had	921:923	arg2	bacteria					943:950	much fewer viable bacteria	925:950	much fewer viable bacteria	925:950	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	5	80	contain	had	921:923	arg1	salads					892:897	rocket salads	885:897	rocket salads from vertical farming	885:919	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	13	81	theme	consumed	2597:2604	arg1	food					2614:2617	a widely consumed vegetal food	2588:2617	a widely consumed vegetal food frequently eaten raw	2588:2638	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	2	82	theme	vegetables	298:307	arg1	consumption					279:289	the consumption	275:289	the consumption of raw vegetables	275:307	It was hypothesized that the consumption of raw vegetables could contribute to the maintenance of the intestinal microbial community structure.
36537820	7	83	theme	traditional	1412:1422	arg1	samples					1432:1438	only traditional farming samples	1407:1438	only traditional farming samples	1407:1438	LAB were isolated from only traditional farming samples and belonged to different species, which were variably distributed among samples and could be partly associated with farming practices.
36537820	4	84	theme	acid	641:644	arg1	LAB					656:658	LAB	656:658	LAB	656:658	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	4	84	theme	acid	641:644	arg1	bacteria					646:653	lactic acid bacteria	634:653	lactic acid bacteria (LAB)	634:659	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	6	85	theme	taxonomic	1329:1337	arg1	composition					1339:1349	the taxonomic composition	1325:1349	the taxonomic composition of rocket bacterial communities	1325:1381	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	13	86	theme	microbiota	2801:2810	arg1	biodiversity					2770:2781	the biodiversity	2766:2781	the biodiversity of the intestinal microbiota	2766:2810	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	5	87	from	farming	913:919	arg1	salads					892:897	rocket salads	885:897	rocket salads from vertical farming	885:919	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	10	88	theme	reduced	2079:2085	arg1	microorganisms					2117:2130	naturally occurring microorganisms	2097:2130	naturally occurring microorganisms	2097:2130	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	10	88	theme	reduced	2079:2085	arg1	amount					2087:2092	a reduced amount	2077:2092	a reduced amount of naturally occurring microorganisms	2077:2130	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	9	89	contain	possess	1882:1888	arg2	ability					1894:1900	the ability to survive gastrointestinal transit	1890:1936	the ability to survive gastrointestinal transit	1890:1936	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	9	89	contain	possess	1882:1888	arg1	bacteria					1868:1875	live bacteria	1863:1875	live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota	1863:2017	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	4	90	theme	rocket	856:861	arg1	salads					863:868	rocket salads	856:868	rocket salads	856:868	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	11	91	from	causes	2374:2379	arg1	world					2407:2411	the developed world	2393:2411	the developed world	2393:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	5	92	theme	rocket	885:890	arg1	salads					892:897	rocket salads	885:897	rocket salads from vertical farming	885:919	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	6	93	theme	several	1184:1190	arg1	taxa					1202:1205	several bacterial taxa	1184:1205	several bacterial taxa	1184:1205	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	8	94	dep	bacteria	1725:1732	arg1	LAB					1748:1750	LAB	1748:1750	particularly LAB	1735:1750	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	10	95	theme	occurring	2107:2115	arg1	microorganisms					2117:2130	naturally occurring microorganisms	2097:2130	naturally occurring microorganisms	2097:2130	IMPORTANCE Western-type diets are composed of foods with a reduced amount of naturally occurring microorganisms.
36537820	5	96	theme	rocket	1024:1029	arg1	salads					1031:1036	rocket salads	1024:1036	rocket salads from traditional farming	1024:1061	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	9	97	theme	commercial	1818:1827	arg1	salads					1849:1854	commercial ready-to-eat rocket salads	1818:1854	commercial ready-to-eat rocket salads	1818:1854	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	4	98	theme	plate	679:683	arg1	agar					691:694	plate count agar	679:694	plate count agar (PCA)	679:700	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	4	98	theme	plate	679:683	arg1	PCA					697:699	PCA	697:699	PCA	697:699	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	5	99	from	farming	1055:1061	arg1	salads					1031:1036	rocket salads	1024:1036	rocket salads from traditional farming	1024:1061	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	1	100	theme	key	142:144	arg1	taxa					156:159	key microbial taxa	142:159	key microbial taxa within the gastrointestinal microbiota	142:198	Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases.
36537820	9	101	theme	rocket	1842:1847	arg1	salads					1849:1854	commercial ready-to-eat rocket salads	1818:1854	commercial ready-to-eat rocket salads	1818:1854	This study suggests that commercial ready-to-eat rocket salads harbor live bacteria that possess the ability to survive gastrointestinal transit, potentially contributing to the taxonomic structure of the human gut microbiota.
36537820	7	102	attach	isolated	1393:1400	arg1	samples					1432:1438	only traditional farming samples	1407:1438	only traditional farming samples	1407:1438	LAB were isolated from only traditional farming samples and belonged to different species, which were variably distributed among samples and could be partly associated with farming practices.
36537820	7	102	attach	isolated	1393:1400	arg2	LAB					1384:1386	LAB	1384:1386	LAB	1384:1386	LAB were isolated from only traditional farming samples and belonged to different species, which were variably distributed among samples and could be partly associated with farming practices.
36537820	11	103	theme	significant	2362:2372	arg1	causes					2374:2379	the most significant causes	2353:2379	the most significant causes of death in the developed world	2353:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	5	104	theme	fewer	930:934	arg1	bacteria					943:950	much fewer viable bacteria	925:950	much fewer viable bacteria	925:950	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	6	105	theme	bacterial	1361:1369	arg1	communities					1371:1381	rocket bacterial communities	1354:1381	rocket bacterial communities	1354:1381	Furthermore, although α- and β-diversity analyses did not differentiate rocket samples according to farming techniques, several bacterial taxa distinguished organic and integrated from conventional farming salads, suggesting that farming practices could affect the taxonomic composition of rocket bacterial communities.
36537820	5	106	theme	viable	936:941	arg1	bacteria					943:950	much fewer viable bacteria	925:950	much fewer viable bacteria	925:950	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	12	107	theme	rocket	2499:2504	arg1	salads					2506:2511	commercial ready-to-eat rocket salads	2475:2511	commercial ready-to-eat rocket salads produced through different farming practices	2475:2556	Here, we studied the microorganisms that are associated with commercial ready-to-eat rocket salads produced through different farming practices.
36537820	3	108	theme	commercial	448:457	arg1	salads					466:471	commercial rocket salads	448:471	commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming	448:590	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	13	109	theme	other	2685:2689	arg1	microbes					2691:2698	other microbes	2685:2698	other microbes	2685:2698	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	3	110	dep	traditional	519:529	arg1	organic					546:552	organic	546:552	organic	546:552	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	3	110	dep	traditional	519:529	arg1	integrated					559:568	integrated	559:568	integrated	559:568	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	3	110	dep	traditional	519:529	arg1	conventional					532:543	conventional	532:543	conventional	532:543	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	14	111	theme	vegetal	2859:2865	arg1	foods					2867:2871	virtually all vegetal foods	2845:2871	virtually all vegetal foods that are consumed raw	2845:2893	This deduction may be valid for virtually all vegetal foods that are consumed raw.
36537820	2	112	theme	structure	383:391	arg1	maintenance					333:343	the maintenance	329:343	the maintenance of the intestinal microbial community structure	329:391	It was hypothesized that the consumption of raw vegetables could contribute to the maintenance of the intestinal microbial community structure.
36537820	4	113	dep	used	809:812	arg1	profile					817:823	profile	817:823	profile	817:823	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	13	114	theme	lactic	2660:2665	arg1	bacteria					2672:2679	lactic acid bacteria	2660:2679	lactic acid bacteria	2660:2679	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	5	115	theme	community	972:980	arg1	structure					982:990	a bacterial community structure	960:990	a bacterial community structure markedly different from that of rocket salads from traditional farming	960:1061	We found that rocket salads from vertical farming had much fewer viable bacteria and had a bacterial community structure markedly different from that of rocket salads from traditional farming.
36537820	12	116	theme	farming	2540:2546	arg1	practices					2548:2556	different farming practices	2530:2556	different farming practices	2530:2556	Here, we studied the microorganisms that are associated with commercial ready-to-eat rocket salads produced through different farming practices.
36537820	4	117	theme	16S	780:782	arg1	sequencing					794:803	16S rRNA gene sequencing	780:803	16S rRNA gene sequencing	780:803	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	0	118	theme	Bacteria	53:60	arg1	Reservoir					40:48	Potential Reservoir	30:48	Potential Reservoir of Bacteria for the Human Microbiome	30:85	Ready-To-Eat Rocket Salads as Potential Reservoir of Bacteria for the Human Microbiome.
36537820	11	119	theme	chronic	2291:2297	arg1	diseases					2320:2327	chronic metabolic and immune diseases	2291:2327	chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world	2291:2411	It was hypothesized that a microbe-depleted diet can favor the alteration of the human intestinal microbial ecosystem, therefore contributing to the onset of chronic metabolic and immune diseases currently recognized as the most significant causes of death in the developed world.
36537820	8	120	theme	in	1611:1612	arg1	simulation					1620:1629	in vitro simulation	1611:1629	in vitro simulation of gastrointestinal digestion	1611:1659	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	4	121	theme	gene	789:792	arg1	sequencing					794:803	16S rRNA gene sequencing	780:803	16S rRNA gene sequencing	780:803	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	8	122	theme	different	1697:1705	arg1	bacteria					1725:1732	rocket-associated bacteria	1707:1732	several taxonomically different rocket-associated bacteria (particularly LAB)	1675:1751	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	4	123	used	used	809:812	arg2	metataxonomics					757:770	metataxonomics	757:770	metataxonomics through 16S rRNA gene sequencing	757:803	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	4	124	theme	bacteria	621:628	arg1	counts					600:605	Viable counts	593:605	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB)	593:659	Viable counts of mesophilic bacteria and lactic acid bacteria (LAB) were performed on plate count agar (PCA) and de Man-Rogosa-Sharpe (MRS) agar at pH 5.7, whereas metataxonomics through 16S rRNA gene sequencing was used to profile total bacteria associated with rocket salads.
36537820	3	125	theme	farming	500:506	arg1	practices					508:516	different farming practices	490:516	different farming practices	490:516	In this context, we explored bacteria associated with commercial rocket salads produced through different farming practices: traditional (conventional, organic, and integrated) and vertical farming.
36537820	8	126	theme	gastrointestinal	1634:1649	arg1	digestion					1651:1659	gastrointestinal digestion	1634:1659	gastrointestinal digestion	1634:1659	Finally, the INFOGEST protocol for in vitro simulation of gastrointestinal digestion revealed that several taxonomically different rocket-associated bacteria (particularly LAB) could survive gastrointestinal transit.
36537820	13	127	dep	salad	2581:2585	arg1	food					2614:2617	a widely consumed vegetal food	2588:2617	a widely consumed vegetal food frequently eaten raw	2588:2638	We showed that rocket salad (a widely consumed vegetal food frequently eaten raw) may be a source of lactic acid bacteria and other microbes that can survive gastrointestinal transit, potentially increasing the biodiversity of the intestinal microbiota.
36537820	2	128	theme	microbial	363:371	arg1	structure					383:391	the intestinal microbial community structure	348:391	the intestinal microbial community structure	348:391	It was hypothesized that the consumption of raw vegetables could contribute to the maintenance of the intestinal microbial community structure.
36537820	1	129	theme	diseases	240:247	arg1	onset					215:219	the onset	211:219	the onset of noncommunicable diseases	211:247	Reportedly, Western-type diets may induce the loss of key microbial taxa within the gastrointestinal microbiota, promoting the onset of noncommunicable diseases.
37321749	2	0	theme	pectin	479:484	arg1	composition					427:437	The cell wall composition	413:437	The cell wall composition	413:437	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	2	0	theme	pectin	479:484	arg1	content					457:463	specifically the content	440:463	specifically the content of lignin and pectin	440:484	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	3	1	theme	short-chain	776:786	arg1	production					799:808	less short-chain fatty acid production	771:808	less short-chain fatty acid production	771:808	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	4	2	theme	similar	855:861	arg1	composition					869:879	similar fibre composition	855:879	similar fibre composition	855:879	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	2	3	theme	lignin	468:473	arg1	composition					427:437	The cell wall composition	413:437	The cell wall composition	413:437	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	2	3	theme	lignin	468:473	arg1	content					457:463	specifically the content	440:463	specifically the content of lignin and pectin	440:484	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	1	4	theme	cell	323:326	arg1	fibres					333:338	cell wall fibres	323:338	cell wall fibres	323:338	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	4	5	theme	coordinate	945:954	arg1	analysis					956:963	the principal coordinate analysis	931:963	the principal coordinate analysis	931:963	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	5	6	theme	relationship	1242:1253	arg1	understanding					1221:1233	a better understanding	1212:1233	a better understanding of the relationship between plant cell walls and gut health	1212:1293	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	6	7	theme	dietary	1381:1387	arg1	interventions					1389:1401	dietary interventions	1381:1401	dietary interventions	1381:1401	This research has practical implications for the development of functional foods and dietary interventions.
37321749	1	8	theme	wall	170:173	arg1	fibres					175:180	plant cell wall fibres	159:180	plant cell wall fibres	159:180	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	9	theme	in	343:344	arg1	profiles					372:379	in vitro faecal fermentation profiles	343:379	in vitro faecal fermentation profiles	343:379	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	6	10	theme	foods	1371:1375	arg1	development					1345:1355	the development	1341:1355	the development of functional foods and dietary interventions	1341:1401	This research has practical implications for the development of functional foods and dietary interventions.
37321749	3	11	theme	cell	599:602	arg1	tuber					622:626	tuber	622:626	tuber	622:626	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	11	theme	cell	599:602	arg1	legume					611:616	legume	611:616	legume	611:616	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	11	theme	cell	599:602	arg1	walls					604:608	cell walls	599:608	type I cell walls (legume and tuber) which have high pectin content	592:658	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	2	12	theme	fermentation	555:566	arg1	outcomes					568:575	the gut microbiota and fermentation outcomes	532:575	outcomes	568:575	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	2	13	theme	cell	417:420	arg1	composition					427:437	The cell wall composition	413:437	The cell wall composition	413:437	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	2	13	theme	cell	417:420	arg1	content					457:463	specifically the content	440:463	specifically the content of lignin and pectin	440:484	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	1	14	dep	barley	207:212	arg1	i.e.					201:204	i.e.	201:204	i.e.	201:204	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	4	15	theme	cell	1010:1013	arg1	walls					1015:1019	cell walls	1010:1019	cell walls	1010:1019	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	1	16	theme	sweet	300:304	arg1	potato					306:311	sweet potato	300:311	sweet potato	300:311	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	3	17	theme	pectin	645:650	arg1	content					652:658	high pectin content	640:658	high pectin content	640:658	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	18	dep	type	665:668	arg1	walls					678:682	cell walls	673:682	the type II cell walls (cereal) which are high in lignin but low in pectin	661:734	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	19	theme	cell	673:676	arg1	walls					678:682	cell walls	673:682	the type II cell walls (cereal) which are high in lignin but low in pectin	661:734	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	5	20	theme	wall	1122:1125	arg1	composition					1127:1137	cell wall composition	1117:1137	cell wall composition	1117:1137	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	1	21	theme	types	150:154	arg1	effect					130:135	the effect	126:135	the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition	126:410	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	22	dep	in	343:344	arg1	vitro					346:350	vitro	346:350	vitro	346:350	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	23	theme	plant	159:163	arg1	fibres					175:180	plant cell wall fibres	159:180	plant cell wall fibres	159:180	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	24	dep	legume	235:240	arg1	pea					249:251	pea	249:251	pea	249:251	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	24	dep	legume	235:240	arg1	bean					259:262	faba bean	254:262	faba bean	254:262	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	24	dep	legume	235:240	arg1	bean					274:277	mung bean	269:277	mung bean	269:277	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	2	25	theme	significant	507:517	arg1	influence					519:527	a significant influence	505:527	a significant influence	505:527	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	1	26	from	effect	130:135	arg1	profiles					372:379	in vitro faecal fermentation profiles	343:379	in vitro faecal fermentation profiles	343:379	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	26	from	effect	130:135	arg1	composition					400:410	gut microbiota composition	385:410	gut microbiota composition	385:410	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	27	dep	pea	249:251	arg1	i.e.					243:246	i.e.	243:246	i.e.	243:246	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	2	28	theme	gut	536:538	arg1	microbiota					540:549	the gut microbiota and fermentation outcomes	532:575	microbiota	540:549	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	0	29	theme	Plant	0:4	arg1	composition					16:26	Plant cell wall composition	0:26	Plant cell wall composition	0:26	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	5	30	theme	gut	1284:1286	arg1	health					1288:1293	gut health	1284:1293	gut health	1284:1293	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	0	31	theme	wall	11:14	arg1	composition					16:26	Plant cell wall composition	0:26	Plant cell wall composition	0:26	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	4	32	theme	redundancy	815:824	arg1	analysis					826:833	The redundancy analysis	811:833	The redundancy analysis	811:833	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	1	33	theme	fermentation	359:370	arg1	profiles					372:379	in vitro faecal fermentation profiles	343:379	in vitro faecal fermentation profiles	343:379	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	0	34	theme	gut	42:44	arg1	microbiota					46:55	gut microbiota	42:55	gut microbiota	42:55	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	3	35	dep	type	592:595	arg1	tuber					622:626	tuber	622:626	tuber	622:626	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	35	dep	type	592:595	arg1	legume					611:616	legume	611:616	legume	611:616	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	35	dep	type	592:595	arg1	walls					604:608	cell walls	599:608	type I cell walls (legume and tuber) which have high pectin content	592:658	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	36	theme	lower	742:746	arg1	rates					761:765	a lower fermentation rates	740:765	a lower fermentation rates	740:765	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	6	37	contain	has	1310:1312	arg1	research					1301:1308	This research	1296:1308	This research	1296:1308	This research has practical implications for the development of functional foods and dietary interventions.
37321749	6	37	contain	has	1310:1312	arg2	implications					1324:1335	practical implications	1314:1335	practical implications for the development of functional foods and dietary interventions	1314:1401	This research has practical implications for the development of functional foods and dietary interventions.
37321749	5	38	theme	better	1214:1219	arg1	understanding					1221:1233	a better understanding	1212:1233	a better understanding of the relationship between plant cell walls and gut health	1212:1293	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	3	39	theme	fatty	788:792	arg1	production					799:808	less short-chain fatty acid production	771:808	less short-chain fatty acid production	771:808	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	1	40	theme	microbiota	389:398	arg1	composition					400:410	gut microbiota composition	385:410	gut microbiota composition	385:410	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	6	41	theme	practical	1314:1322	arg1	implications					1324:1335	practical implications	1314:1335	practical implications for the development of functional foods and dietary interventions	1314:1401	This research has practical implications for the development of functional foods and dietary interventions.
37321749	4	42	theme	fibre	863:867	arg1	composition					869:879	similar fibre composition	855:879	similar fibre composition	855:879	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	1	43	theme	wall	328:331	arg1	fibres					333:338	cell wall fibres	323:338	cell wall fibres	323:338	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	6	44	theme	interventions	1389:1401	arg1	development					1345:1355	the development	1341:1355	the development of functional foods and dietary interventions	1341:1401	This research has practical implications for the development of functional foods and dietary interventions.
37321749	1	45	theme	cell	165:168	arg1	fibres					175:180	plant cell wall fibres	159:180	plant cell wall fibres	159:180	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	3	46	from	high	703:706	arg1	pectin					729:734	pectin	729:734	pectin	729:734	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	46	from	high	703:706	arg1	lignin					711:716	lignin	711:716	lignin	711:716	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	2	47	contain	have	500:503	arg1	composition					427:437	The cell wall composition	413:437	The cell wall composition	413:437	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	2	47	contain	have	500:503	arg2	influence					519:527	a significant influence	505:527	a significant influence	505:527	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	2	47	contain	have	500:503	arg1	content					457:463	specifically the content	440:463	specifically the content of lignin and pectin	440:484	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	4	48	dep	showed	835:840	arg1	clustered					907:915	clustered	907:915	clustered together	907:924	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	1	49	theme	fibres	175:180	arg1	types					150:154	different types	140:154	different types	140:154	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	49	theme	fibres	175:180	arg1	tuber					285:289	tuber	285:289	tuber (potato, sweet potato, and yam)	285:321	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	49	theme	fibres	175:180	arg1	legume					235:240	legume	235:240	legume	235:240	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	49	theme	fibres	175:180	arg1	cereal					193:198	cereal	193:198	cereal	193:198	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	5	50	theme	plant	1263:1267	arg1	walls					1274:1278	plant cell walls	1263:1278	plant cell walls	1263:1278	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	5	51	theme	cell	1269:1272	arg1	walls					1274:1278	plant cell walls	1263:1278	plant cell walls	1263:1278	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	6	52	theme	functional	1360:1369	arg1	foods					1371:1375	functional foods	1360:1375	functional foods	1360:1375	This research has practical implications for the development of functional foods and dietary interventions.
37321749	2	53	theme	wall	422:425	arg1	composition					427:437	The cell wall composition	413:437	The cell wall composition	413:437	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	2	53	theme	wall	422:425	arg1	content					457:463	specifically the content	440:463	specifically the content of lignin and pectin	440:484	The cell wall composition, specifically the content of lignin and pectin, was found to have a significant influence on the gut microbiota and fermentation outcomes.
37321749	4	54	theme	principal	935:943	arg1	analysis					956:963	the principal coordinate analysis	931:963	the principal coordinate analysis	931:963	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	3	55	contain	had	736:738	arg2	production					799:808	less short-chain fatty acid production	771:808	less short-chain fatty acid production	771:808	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	55	contain	had	736:738	arg1	type					665:668	the type II cell walls (cereal) which are high in lignin but low in pectin	661:734	the type II cell walls (cereal) which are high in lignin but low in pectin	661:734	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	55	contain	had	736:738	arg2	rates					761:765	a lower fermentation rates	740:765	a lower fermentation rates	740:765	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	0	56	from	metabolites	61:71	arg1	fermentation					85:96	in-vitro fermentation	76:96	in-vitro fermentation	76:96	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	3	57	from	pectin	729:734	arg1	high					703:706	high	703:706	high	703:706	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	4	58	theme	walls	1015:1019	arg1	types					1001:1005	different types	991:1005	different types of cell walls	991:1019	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	4	59	theme	closer	1025:1030	arg1	proximity					1032:1040	closer proximity	1025:1040	closer proximity among the same cell wall types	1025:1071	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	4	60	theme	cell	1057:1060	arg1	types					1067:1071	the same cell wall types	1048:1071	the same cell wall types	1048:1071	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	3	61	from	lignin	711:716	arg1	high					703:706	high	703:706	high	703:706	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	4	62	theme	different	991:999	arg1	types					1001:1005	different types	991:1005	different types of cell walls	991:1019	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	5	63	theme	cell	1117:1120	arg1	composition					1127:1137	cell wall composition	1117:1137	cell wall composition	1117:1137	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	1	64	theme	different	140:148	arg1	types					150:154	different types	140:154	different types	140:154	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	64	theme	different	140:148	arg1	tuber					285:289	tuber	285:289	tuber (potato, sweet potato, and yam)	285:321	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	64	theme	different	140:148	arg1	legume					235:240	legume	235:240	legume	235:240	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	64	theme	different	140:148	arg1	cereal					193:198	cereal	193:198	cereal	193:198	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	3	65	theme	high	640:643	arg1	content					652:658	high pectin content	640:658	high pectin content	640:658	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	0	66	theme	in-vitro	76:83	arg1	fermentation					85:96	in-vitro fermentation	76:96	in-vitro fermentation	76:96	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	3	67	dep	walls	604:608	arg1	tuber					622:626	tuber	622:626	tuber	622:626	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	67	dep	walls	604:608	arg1	legume					611:616	legume	611:616	legume	611:616	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	67	dep	walls	604:608	arg1	walls					604:608	cell walls	599:608	type I cell walls (legume and tuber) which have high pectin content	592:658	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	4	68	theme	wall	1062:1065	arg1	types					1067:1071	the same cell wall types	1048:1071	the same cell wall types	1048:1071	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	1	69	theme	mung	269:272	arg1	bean					274:277	mung bean	269:277	mung bean	269:277	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	3	70	dep	walls	678:682	arg1	cereal					685:690	cereal	685:690	cereal	685:690	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	0	71	from	microbiota	46:55	arg1	fermentation					85:96	in-vitro fermentation	76:96	in-vitro fermentation	76:96	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	5	72	theme	microbial	1154:1162	arg1	community					1164:1172	the microbial community	1150:1172	the microbial community	1150:1172	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	0	73	theme	cell	6:9	arg1	composition					16:26	Plant cell wall composition	0:26	Plant cell wall composition	0:26	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	4	74	theme	same	1052:1055	arg1	types					1067:1071	the same cell wall types	1048:1071	the same cell wall types	1048:1071	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	5	75	theme	composition	1127:1137	arg1	importance					1103:1112	the importance	1099:1112	the importance of cell wall composition in shaping the microbial community during fermentation	1099:1192	These findings emphasize the importance of cell wall composition in shaping the microbial community during fermentation and contribute to a better understanding of the relationship between plant cell walls and gut health.
37321749	0	76	dep	microbiota	46:55	arg1	the					38:40	the	38:40	the	38:40	Plant cell wall composition modulates the gut microbiota and metabolites in in-vitro fermentation.
37321749	1	77	theme	faecal	352:357	arg1	profiles					372:379	in vitro faecal fermentation profiles	343:379	in vitro faecal fermentation profiles	343:379	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	78	theme	faba	254:257	arg1	bean					259:262	faba bean	254:262	faba bean	254:262	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	3	79	theme	fermentation	748:759	arg1	rates					761:765	a lower fermentation rates	740:765	a lower fermentation rates	740:765	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	4	80	theme	fermentation	885:896	arg1	profiles					898:905	fermentation profiles	885:905	fermentation profiles	885:905	The redundancy analysis showed samples with similar fibre composition and fermentation profiles clustered together, and the principal coordinate analysis revealed separation among different types of cell walls and closer proximity among the same cell wall types.
37321749	3	81	contain	have	635:638	arg2	content					652:658	high pectin content	640:658	high pectin content	640:658	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	3	81	contain	have	635:638	arg1	type					592:595	type I cell walls (legume and tuber)	592:627	type I cell walls (legume and tuber) which have high pectin content	592:658	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	1	82	theme	gut	385:387	arg1	composition					400:410	gut microbiota composition	385:410	gut microbiota composition	385:410	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	3	83	theme	acid	794:797	arg1	production					799:808	less short-chain fatty acid production	771:808	less short-chain fatty acid production	771:808	Compared with type I cell walls (legume and tuber) which have high pectin content, the type II cell walls (cereal) which are high in lignin but low in pectin had a lower fermentation rates and less short-chain fatty acid production.
37321749	1	84	dep	tuber	285:289	arg1	potato					292:297	potato	292:297	potato	292:297	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	84	dep	tuber	285:289	arg1	potato					306:311	sweet potato	300:311	sweet potato	300:311	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	84	dep	tuber	285:289	arg1	yam					318:320	yam	318:320	yam	318:320	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	85	dep	cereal	193:198	arg1	sorghum					215:221	sorghum	215:221	sorghum	215:221	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	85	dep	cereal	193:198	arg1	rice					228:231	rice	228:231	rice	228:231	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	85	dep	cereal	193:198	arg1	barley					207:212	barley	207:212	barley	207:212	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
37321749	1	85	dep	cereal	193:198	arg1	fibres					333:338	cell wall fibres	323:338	cell wall fibres	323:338	This research investigated the effect of different types of plant cell wall fibres, including cereal (i.e., barley, sorghum, and rice), legume (i.e., pea, faba bean, and mung bean), and tuber (potato, sweet potato, and yam) cell wall fibres on in vitro faecal fermentation profiles and gut microbiota composition.
36700289	0	0	from	outcomes	15:22	arg1	patients					56:63	patients	56:63	patients with contraindication to full anticoagulation due to bleeding	56:125	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	7	1	theme	intermediate	991:1002	arg1	doses					1004:1008	intermediate doses	991:1008	intermediate doses	991:1008	PE occurred in only one of the 4 untreated subjects (25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0).
36700289	12	2	theme	logistic	1517:1524	arg1	P=0.01					1537:1542	logistic regression P=0.01	1517:1542	logistic regression P=0.01	1517:1542	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	1	3	theme	observational	156:168	arg1	study					170:174	This prospective observational study	139:174	This prospective observational study	139:174	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	7	4	theme	subjects	930:937	arg1	subjects					930:937	the 4 untreated subjects	914:937	the 4 untreated subjects	914:937	PE occurred in only one of the 4 untreated subjects (25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0).
36700289	7	4	theme	subjects	930:937	arg1	one					907:909	one	907:909	one	907:909	PE occurred in only one of the 4 untreated subjects (25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0).
36700289	1	5	theme	vein	254:257	arg1	thrombosis					259:268	isolated distal deep vein thrombosis	233:268	isolated distal deep vein thrombosis (IDDVT)	233:276	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	1	5	theme	vein	254:257	arg1	IDDVT					271:275	IDDVT	271:275	IDDVT	271:275	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	5	6	theme	study	702:706	arg1	period					708:713	the study period	698:713	the study period (30 days)	698:723	RESULTS 90/95 patients completed the study period (30 days).
36700289	5	6	theme	study	702:706	arg1	days					719:722	30 days	716:722	30 days	716:722	RESULTS 90/95 patients completed the study period (30 days).
36700289	8	7	dep	occurred	1036:1043	arg1	%					1120:1120	19/29, 65.5%	1109:1120	19/29, 65.5%	1109:1120	Recurrent IDDVTs occurred in 29 subjects (32.2%), more frequently during enoxaparin 4000 (19/29, 65.5%).
36700289	12	8	theme	PDVT	1472:1475	arg1	risk					1464:1467	the risk	1460:1467	the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01)	1460:1543	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	11	9	theme	Enoxaparin	1263:1272	arg1	units					1279:1283	Enoxaparin 4000 units	1263:1283	Enoxaparin 4000 units	1263:1283	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	0	10	theme	full	90:93	arg1	anticoagulation					95:109	full anticoagulation	90:109	full anticoagulation	90:109	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	0	11	from	Management	0:9	arg1	patients					56:63	patients	56:63	patients with contraindication to full anticoagulation due to bleeding	56:125	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	10	12	theme	intermediate	1222:1233	arg1	doses					1235:1239	intermediate doses	1222:1239	intermediate doses (0 vs. 17.8%; P=1.0)	1222:1260	Bleeding complications occurred in 8 subjects (8.9%), all treated with intermediate doses (0 vs. 17.8%; P=1.0).
36700289	3	13	theme	bleeding	515:522	arg1	complications					524:536	bleeding complications	515:536	bleeding complications during the treatment	515:557	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	14	14	theme	daily	1707:1711	arg1	units					1701:1705	CONCLUSIONS Enoxaparin 4000 units daily	1673:1711	CONCLUSIONS Enoxaparin 4000 units daily	1673:1711	CONCLUSIONS Enoxaparin 4000 units daily, supported by US, may be an option for selected patients.
36700289	14	14	theme	daily	1707:1711	arg1	option					1741:1746	an option	1738:1746	an option for selected patients	1738:1768	CONCLUSIONS Enoxaparin 4000 units daily, supported by US, may be an option for selected patients.
36700289	8	15	theme	Recurrent	1019:1027	arg1	IDDVTs					1029:1034	Recurrent IDDVTs	1019:1034	Recurrent IDDVTs	1019:1034	Recurrent IDDVTs occurred in 29 subjects (32.2%), more frequently during enoxaparin 4000 (19/29, 65.5%).
36700289	0	16	with	patients	56:63	arg1	contraindication					70:85	contraindication	70:85	contraindication to full anticoagulation due to bleeding	70:125	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	1	17	dep	BACKGROUND	128:137	arg1	aimed					180:184	aimed	180:184	was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding	176:300	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	12	18	dep	%	1490:1490	arg1	2.82-427.51					1496:1506	2.82-427.51	1496:1506	2.82-427.51	1496:1506	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	12	18	dep	%	1490:1490	arg1	P=0.01					1537:1542	logistic regression P=0.01	1517:1542	logistic regression P=0.01	1517:1542	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	7	19	theme	enoxaparin	966:975	arg1	units					982:986	enoxaparin 4000 units	966:986	enoxaparin 4000 units	966:986	PE occurred in only one of the 4 untreated subjects (25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0).
36700289	13	20	theme	major	1560:1564	arg1	trauma					1566:1571	major trauma	1560:1571	major trauma	1560:1571	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	10	21	dep	doses	1235:1239	arg1	%					1252:1252	0 vs. 17.8%	1242:1252	0 vs. 17.8%; P=1.0	1242:1259	Bleeding complications occurred in 8 subjects (8.9%), all treated with intermediate doses (0 vs. 17.8%; P=1.0).
36700289	6	22	theme	intermediate	819:830	arg1	doses					832:836	intermediate doses	819:836	intermediate doses respectively	819:849	PDVT occurred in 2/41 (4.9%) and in 3/45 (6.7%) subjects receiving enoxaparin 4000 units and intermediate doses respectively (OR 1.39; 95% CI: 0.22-11; P=0.72).
36700289	7	23	dep	patients	950:957	arg1	P=1.0					1011:1015	P=1.0	1011:1015	P=1.0	1011:1015	PE occurred in only one of the 4 untreated subjects (25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0).
36700289	11	24	theme	outcome	1333:1339	arg1	risk					1311:1314	the risk	1307:1314	the risk of the composite outcome	1307:1339	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	11	25	dep	doses	1362:1366	arg1	%					1372:1372	4.9%	1369:1372	4.9%	1369:1372	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	11	25	dep	doses	1362:1366	arg1	%					1382:1382	24.4%	1378:1382	24.4%	1378:1382	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	11	25	dep	doses	1362:1366	arg1	%					1396:1396	6.31; 95%	1388:1396	6.31; 95%	1388:1396	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	2	26	theme	ultrasound	384:393	arg1	surveillance					395:406	ultrasound surveillance	384:406	ultrasound surveillance (US)	384:411	METHODS Patients received enoxaparin 4000 units daily or intermediate doses, and ultrasound surveillance (US).
36700289	13	27	dep	%	1618:1618	arg1	P=0.03					1664:1669	logistic regression P=0.03	1644:1669	5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03	1613:1669	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	14	28	theme	Enoxaparin	1685:1694	arg1	units					1701:1705	CONCLUSIONS Enoxaparin 4000 units daily	1673:1711	CONCLUSIONS Enoxaparin 4000 units daily	1673:1711	CONCLUSIONS Enoxaparin 4000 units daily, supported by US, may be an option for selected patients.
36700289	14	28	theme	Enoxaparin	1685:1694	arg1	option					1741:1746	an option	1738:1746	an option for selected patients	1738:1768	CONCLUSIONS Enoxaparin 4000 units daily, supported by US, may be an option for selected patients.
36700289	9	29	dep	died	1138:1141	arg1	%					1147:1147	4.4%	1144:1147	4.4%	1144:1147	Four patients died (4.4%).
36700289	10	30	dep	%	1252:1252	arg1	P=1.0					1255:1259	P=1.0	1255:1259	0 vs. 17.8%; P=1.0	1242:1259	Bleeding complications occurred in 8 subjects (8.9%), all treated with intermediate doses (0 vs. 17.8%; P=1.0).
36700289	11	31	theme	composite	1323:1331	arg1	outcome					1333:1339	the composite outcome	1319:1339	the composite outcome	1319:1339	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	2	32	theme	METHODS	303:309	arg1	Patients					311:318	METHODS Patients	303:318	METHODS Patients	303:318	METHODS Patients received enoxaparin 4000 units daily or intermediate doses, and ultrasound surveillance (US).
36700289	11	33	dep	%	1372:1372	arg1	P=0.02					1414:1419	P=0.02	1414:1419	4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02	1369:1419	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	1	34	theme	early	199:203	arg1	outcomes					205:212	early outcomes	199:212	early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding	199:300	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	1	35	with	inpatients	217:226	arg1	thrombosis					259:268	isolated distal deep vein thrombosis	233:268	isolated distal deep vein thrombosis (IDDVT)	233:276	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	1	35	with	inpatients	217:226	arg1	bleeding					293:300	bleeding	293:300	bleeding	293:300	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	1	35	with	inpatients	217:226	arg1	IDDVT					271:275	IDDVT	271:275	IDDVT	271:275	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	2	36	dep	units	345:349	arg1	doses					373:377	daily or intermediate doses	351:377	enoxaparin 4000 units daily or intermediate doses	329:377	METHODS Patients received enoxaparin 4000 units daily or intermediate doses, and ultrasound surveillance (US).
36700289	7	37	dep	occurred	890:897	arg1	patients					950:957	25% vs. 0 patients	940:957	25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0	940:1015	PE occurred in only one of the 4 untreated subjects (25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0).
36700289	4	38	theme	Secondary	613:621	arg1	outcomes					623:630	Secondary outcomes	613:630	Secondary outcomes	613:630	Secondary outcomes were recurrent IDDVTs and death.
36700289	0	39	theme	deep	32:35	arg1	thrombosis					42:51	calf deep vein thrombosis	27:51	calf deep vein thrombosis	27:51	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	13	40	dep	risk	1604:1607	arg1	P=0.04					1636:1641	P=0.04	1636:1641	P=0.04	1636:1641	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	13	40	dep	risk	1604:1607	arg1	%					1618:1618	5; 95% CI: 1.06-23.76	1613:1633	5; 95% CI: 1.06-23.76	1613:1633	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	13	41	theme	bleeding	1595:1602	arg1	risk					1604:1607	increased bleeding risk	1585:1607	increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03)	1585:1670	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	4	42	theme	recurrent	637:645	arg1	IDDVTs					647:652	recurrent IDDVTs	637:652	recurrent IDDVTs	637:652	Secondary outcomes were recurrent IDDVTs and death.
36700289	0	43	theme	calf	27:30	arg1	thrombosis					42:51	calf deep vein thrombosis	27:51	calf deep vein thrombosis	27:51	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	3	44	theme	Primary	414:420	arg1	outcomes					422:429	Primary outcomes	414:429	Primary outcomes	414:429	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	6	45	theme	enoxaparin	793:802	arg1	units					809:813	enoxaparin 4000 units	793:813	enoxaparin 4000 units	793:813	PDVT occurred in 2/41 (4.9%) and in 3/45 (6.7%) subjects receiving enoxaparin 4000 units and intermediate doses respectively (OR 1.39; 95% CI: 0.22-11; P=0.72).
36700289	3	46	theme	PDVT	580:583	arg1	extension					436:444	extension	436:444	extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE)	436:512	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	46	theme	PDVT	580:583	arg1	PDVT					580:583	PDVT	580:583	PDVT	580:583	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	46	theme	PDVT	580:583	arg1	complications					524:536	bleeding complications	515:536	bleeding complications during the treatment	515:557	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	46	theme	PDVT	580:583	arg1	complications					598:610	bleeding complications	589:610	bleeding complications	589:610	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	46	theme	PDVT	580:583	arg1	composite					567:575	the composite	563:575	the composite of PDVT and bleeding complications	563:610	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	10	47	theme	Bleeding	1151:1158	arg1	complications					1160:1172	Bleeding complications	1151:1172	Bleeding complications	1151:1172	Bleeding complications occurred in 8 subjects (8.9%), all treated with intermediate doses (0 vs. 17.8%; P=1.0).
36700289	1	48	theme	inpatients	217:226	arg1	outcomes					205:212	early outcomes	199:212	early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding	199:300	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	0	49	theme	thrombosis	42:51	arg1	outcomes					15:22	outcomes	15:22	outcomes	15:22	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	0	49	theme	thrombosis	42:51	arg1	Management					0:9	Management	0:9	Management	0:9	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	3	50	theme	pulmonary	490:498	arg1	embolism					500:507	symptomatic pulmonary embolism	478:507	symptomatic pulmonary embolism (PE)	478:512	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	50	theme	pulmonary	490:498	arg1	PE					510:511	PE	510:511	PE	510:511	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	5	51	theme	RESULTS	665:671	arg1	patients					679:686	RESULTS 90/95 patients	665:686	RESULTS 90/95 patients	665:686	RESULTS 90/95 patients completed the study period (30 days).
36700289	6	52	dep	CI	865:866	arg1	P=0.72					878:883	P=0.72	878:883	P=0.72	878:883	PDVT occurred in 2/41 (4.9%) and in 3/45 (6.7%) subjects receiving enoxaparin 4000 units and intermediate doses respectively (OR 1.39; 95% CI: 0.22-11; P=0.72).
36700289	6	52	dep	CI	865:866	arg1	0.22-11					869:875	0.22-11	869:875	0.22-11	869:875	PDVT occurred in 2/41 (4.9%) and in 3/45 (6.7%) subjects receiving enoxaparin 4000 units and intermediate doses respectively (OR 1.39; 95% CI: 0.22-11; P=0.72).
36700289	13	53	theme	logistic	1644:1651	arg1	P=0.03					1664:1669	logistic regression P=0.03	1644:1669	5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03	1613:1669	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	2	54	theme	daily	351:355	arg1	doses					373:377	daily or intermediate doses	351:377	enoxaparin 4000 units daily or intermediate doses	329:377	METHODS Patients received enoxaparin 4000 units daily or intermediate doses, and ultrasound surveillance (US).
36700289	0	55	theme	vein	37:40	arg1	thrombosis					42:51	calf deep vein thrombosis	27:51	calf deep vein thrombosis	27:51	Management and outcomes of calf deep vein thrombosis in patients with contraindication to full anticoagulation due to bleeding.
36700289	6	56	theme	%	863:863	arg1	CI					865:866	1.39; 95% CI	855:866	1.39; 95% CI: 0.22-11; P=0.72	855:883	PDVT occurred in 2/41 (4.9%) and in 3/45 (6.7%) subjects receiving enoxaparin 4000 units and intermediate doses respectively (OR 1.39; 95% CI: 0.22-11; P=0.72).
36700289	14	57	theme	CONCLUSIONS	1673:1683	arg1	units					1701:1705	CONCLUSIONS Enoxaparin 4000 units daily	1673:1711	CONCLUSIONS Enoxaparin 4000 units daily	1673:1711	CONCLUSIONS Enoxaparin 4000 units daily, supported by US, may be an option for selected patients.
36700289	14	57	theme	CONCLUSIONS	1673:1683	arg1	option					1741:1746	an option	1738:1746	an option for selected patients	1738:1768	CONCLUSIONS Enoxaparin 4000 units daily, supported by US, may be an option for selected patients.
36700289	13	58	theme	regression	1653:1662	arg1	P=0.03					1664:1669	logistic regression P=0.03	1644:1669	5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03	1613:1669	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	3	59	theme	symptomatic	478:488	arg1	embolism					500:507	symptomatic pulmonary embolism	478:507	symptomatic pulmonary embolism (PE)	478:512	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	59	theme	symptomatic	478:488	arg1	PE					510:511	PE	510:511	PE	510:511	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	12	60	dep	risk	1464:1467	arg1	P=0.01					1509:1514	P=0.01	1509:1514	P=0.01	1509:1514	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	12	60	dep	risk	1464:1467	arg1	%					1490:1490	20.92; 95% CI	1481:1493	20.92; 95% CI	1481:1493	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	3	61	theme	bleeding	589:596	arg1	complications					598:610	bleeding complications	589:610	bleeding complications	589:610	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	12	62	theme	regression	1526:1535	arg1	P=0.01					1537:1542	logistic regression P=0.01	1517:1542	logistic regression P=0.01	1517:1542	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	13	63	with	Patients	1546:1553	arg1	trauma					1566:1571	major trauma	1560:1571	major trauma	1560:1571	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	3	64	theme	complications	598:610	arg1	extension					436:444	extension	436:444	extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE)	436:512	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	64	theme	complications	598:610	arg1	PDVT					580:583	PDVT	580:583	PDVT	580:583	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	64	theme	complications	598:610	arg1	complications					524:536	bleeding complications	515:536	bleeding complications during the treatment	515:557	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	64	theme	complications	598:610	arg1	complications					598:610	bleeding complications	589:610	bleeding complications	589:610	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	64	theme	complications	598:610	arg1	composite					567:575	the composite	563:575	the composite of PDVT and bleeding complications	563:610	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	11	65	theme	higher	1355:1360	arg1	doses					1362:1366	higher doses	1355:1366	higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02)	1355:1420	Enoxaparin 4000 units significantly reduced the risk of the composite outcome compared with higher doses (4.9% vs. 24.4%; OR 6.31; 95% CI: 1.56-42.65; P=0.02).
36700289	7	66	theme	untreated	920:928	arg1	subjects					930:937	the 4 untreated subjects	914:937	the 4 untreated subjects	914:937	PE occurred in only one of the 4 untreated subjects (25% vs. 0 patients taking enoxaparin 4000 units or intermediate doses; P=1.0).
36700289	2	67	theme	enoxaparin	329:338	arg1	units					345:349	enoxaparin 4000 units	329:349	enoxaparin 4000 units daily or intermediate doses	329:377	METHODS Patients received enoxaparin 4000 units daily or intermediate doses, and ultrasound surveillance (US).
36700289	13	68	theme	increased	1585:1593	arg1	risk					1604:1607	increased bleeding risk	1585:1607	increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03)	1585:1670	Patients with major trauma are also at increased bleeding risk (OR 5; 95% CI: 1.06-23.76, P=0.04; logistic regression P=0.03).
36700289	1	69	theme	isolated	233:240	arg1	thrombosis					259:268	isolated distal deep vein thrombosis	233:268	isolated distal deep vein thrombosis (IDDVT)	233:276	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	1	69	theme	isolated	233:240	arg1	IDDVT					271:275	IDDVT	271:275	IDDVT	271:275	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	3	70	theme	popliteal	453:461	arg1	PDVT					469:472	PDVT	469:472	PDVT	469:472	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	3	70	theme	popliteal	453:461	arg1	vein					463:466	the popliteal vein	449:466	the popliteal vein (PDVT)	449:473	Primary outcomes were extension to the popliteal vein (PDVT) or symptomatic pulmonary embolism (PE), bleeding complications during the treatment and the composite of PDVT and bleeding complications.
36700289	2	71	theme	intermediate	360:371	arg1	doses					373:377	daily or intermediate doses	351:377	enoxaparin 4000 units daily or intermediate doses	329:377	METHODS Patients received enoxaparin 4000 units daily or intermediate doses, and ultrasound surveillance (US).
36700289	12	72	theme	Major	1423:1427	arg1	trauma					1429:1434	Major trauma	1423:1434	Major trauma	1423:1434	Major trauma significantly increased the risk of PDVT (OR 20.92; 95% CI: 2.82-427.51, P=0.01; logistic regression P=0.01).
36700289	1	73	theme	distal	242:247	arg1	thrombosis					259:268	isolated distal deep vein thrombosis	233:268	isolated distal deep vein thrombosis (IDDVT)	233:276	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	1	73	theme	distal	242:247	arg1	IDDVT					271:275	IDDVT	271:275	IDDVT	271:275	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	14	74	theme	selected	1752:1759	arg1	patients					1761:1768	selected patients	1752:1768	selected patients	1752:1768	CONCLUSIONS Enoxaparin 4000 units daily, supported by US, may be an option for selected patients.
36700289	2	75	dep	surveillance	395:406	arg1	US					409:410	US	409:410	US	409:410	METHODS Patients received enoxaparin 4000 units daily or intermediate doses, and ultrasound surveillance (US).
36700289	1	76	theme	prospective	144:154	arg1	study					170:174	This prospective observational study	139:174	This prospective observational study	139:174	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	6	77	dep	occurred	731:738	arg1	CI					865:866	1.39; 95% CI	855:866	1.39; 95% CI: 0.22-11; P=0.72	855:883	PDVT occurred in 2/41 (4.9%) and in 3/45 (6.7%) subjects receiving enoxaparin 4000 units and intermediate doses respectively (OR 1.39; 95% CI: 0.22-11; P=0.72).
36700289	1	78	theme	deep	249:252	arg1	thrombosis					259:268	isolated distal deep vein thrombosis	233:268	isolated distal deep vein thrombosis (IDDVT)	233:276	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36700289	1	78	theme	deep	249:252	arg1	IDDVT					271:275	IDDVT	271:275	IDDVT	271:275	BACKGROUND This prospective observational study was aimed at assessing early outcomes of inpatients with isolated distal deep vein thrombosis (IDDVT) and coexisting bleeding.
36687691	6	0	theme	protective	986:995	arg1	effect					997:1002	the DNA damage protective effect	971:1002	the DNA damage protective effect	971:1002	The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect.
36687691	9	1	contain	had	1453:1455	arg2	content					1520:1526	the highest sugar content	1502:1526	the highest sugar content (61.94 ± 1.86%)	1502:1542	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	1	contain	had	1453:1455	arg2	effect					1575:1580	the most potent scavenging effect	1548:1580	the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1548:1644	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	1	contain	had	1453:1455	arg2	%					1541:1541	61.94 ± 1.86%	1529:1541	61.94 ± 1.86%	1529:1541	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	1	contain	had	1453:1455	arg1	polysaccharides					1411:1425	Noni polysaccharides	1406:1425	Noni polysaccharides extracted at stage 5 (N5)	1406:1451	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	1	contain	had	1453:1455	arg2	%					1498:1498	8.26 ± 0.14%	1487:1498	8.26 ± 0.14%	1487:1498	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	1	contain	had	1453:1455	arg2	yield					1480:1484	the largest extraction yield	1457:1484	the largest extraction yield (8.26 ± 0.14%)	1457:1499	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	3	2	theme	Noni	562:565	arg1	polysaccharides					567:581	Noni polysaccharides	562:581	Noni polysaccharides	562:581	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	11	3	theme	highest	1864:1870	arg1	%					1904:1904	4.10 ± 0.12%	1893:1904	4.10 ± 0.12%	1893:1904	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	3	theme	highest	1864:1870	arg1	content					1884:1890	the highest uronic acid content	1860:1890	the highest uronic acid content (4.10 ± 0.12%)	1860:1905	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	9	4	theme	±	1535:1535	arg1	%					1541:1541	61.94 ± 1.86%	1529:1541	61.94 ± 1.86%	1529:1541	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	4	theme	±	1535:1535	arg1	content					1520:1526	the highest sugar content	1502:1526	the highest sugar content (61.94 ± 1.86%)	1502:1542	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	1	5	dep	Morinda	152:158	arg1	L					171:171	citrifolia L	160:171	Introduction Morinda citrifolia L. (Noni) as an evergreen plant	139:201	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	3	6	theme	chemical	611:618	arg1	assays					620:625	different chemical assays	601:625	different chemical assays	601:625	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	1	7	theme	polysaccharides	231:245	arg1	Introduction					139:150	Introduction Morinda citrifolia L. (Noni)	139:179	Introduction Morinda citrifolia L. (Noni) as an evergreen plant	139:201	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	1	7	theme	polysaccharides	231:245	arg1	source					213:218	a rich source	206:218	a rich source of natural polysaccharides	206:245	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	2	8	from	changes	318:324	arg1	polysaccharides					329:343	polysaccharides	329:343	polysaccharides of Morinda citrifolia L. (Noni)	329:375	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	10	9	theme	higher	1738:1743	arg1	content					1745:1751	its higher content	1734:1751	its higher content of fucose and rhamnose	1734:1774	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	15	10	theme	valuable	2406:2413	arg1	information					2415:2425	valuable information	2406:2425	valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications	2406:2521	This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
36687691	15	11	theme	various	2491:2497	arg1	applications					2510:2521	various industrial applications	2491:2521	various industrial applications	2491:2521	This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
36687691	0	12	theme	different	109:117	arg1	stages					119:124	different stages	109:124	different stages of maturity	109:136	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	6	13	theme	damage	979:984	arg1	effect					997:1002	the DNA damage protective effect	971:1002	the DNA damage protective effect	971:1002	The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect.
36687691	9	14	theme	scavenging	1564:1573	arg1	effect					1575:1580	the most potent scavenging effect	1548:1580	the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1548:1644	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	8	15	theme	radical	1346:1352	arg1	contents					1354:1361	the sulfuric radical contents	1333:1361	the sulfuric radical contents	1333:1361	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	14	16	theme	high-quality	2340:2351	arg1	extraction					2374:2383	high-quality Noni polysaccharides extraction	2340:2383	high-quality Noni polysaccharides extraction	2340:2383	Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction.
36687691	11	17	theme	superior	1916:1923	arg1	performance					1925:1935	the superior performance	1912:1935	the superior performance in scavenging hydroxyl radicals and protecting DNA	1912:1986	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	9	18	theme	Noni	1406:1409	arg1	polysaccharides					1411:1425	Noni polysaccharides	1406:1425	Noni polysaccharides extracted at stage 5 (N5)	1406:1451	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	12	19	theme	counterpart	2115:2125	arg1	percentage					2076:2085	its higher percentage	2065:2085	its higher percentage of the low molecular weight counterpart	2065:2125	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	10	20	theme	molecular	1788:1796	arg1	weight					1798:1803	smaller molecular weight	1780:1803	smaller molecular weight	1780:1803	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	9	21	dep	IC50	1619:1622	arg1	mg/mL					1630:1634	1.22 mg/mL	1625:1634	IC50: 1.22 mg/mL	1619:1634	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	3	22	theme	chemical	480:487	arg1	composition					489:499	The chemical composition	476:499	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides	468:581	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	11	23	theme	4.10	1893:1896	arg1	%					1904:1904	4.10 ± 0.12%	1893:1904	4.10 ± 0.12%	1893:1904	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	23	theme	4.10	1893:1896	arg1	content					1884:1890	the highest uronic acid content	1860:1890	the highest uronic acid content (4.10 ± 0.12%)	1860:1905	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	24	theme	uronic	1872:1877	arg1	%					1904:1904	4.10 ± 0.12%	1893:1904	4.10 ± 0.12%	1893:1904	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	24	theme	uronic	1872:1877	arg1	content					1884:1890	the highest uronic acid content	1860:1890	the highest uronic acid content (4.10 ± 0.12%)	1860:1905	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	8	25	theme	structure	1379:1387	arg1	characteristics					1389:1403	preliminary structure characteristics	1367:1403	preliminary structure characteristics	1367:1403	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	weights					1110:1116	molecular weights	1100:1116	molecular weights	1100:1116	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	effects					1243:1249	DNA protective effects	1228:1249	DNA protective effects of Noni polysaccharides	1228:1273	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	levels					1147:1152	sugar levels	1141:1152	sugar levels	1141:1152	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	capacities					1212:1221	antioxidant capacities	1200:1221	antioxidant capacities	1200:1221	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	contents					1131:1138	uronic acid contents	1119:1138	uronic acid contents	1119:1138	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	compositions					1170:1181	monosaccharide compositions	1155:1181	monosaccharide compositions	1155:1181	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	yields					1092:1097	the extraction yields	1077:1097	the extraction yields	1077:1097	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	26	theme	polysaccharides	1259:1273	arg1	proportions					1187:1197	proportions	1187:1197	proportions	1187:1197	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	12	27	theme	greater	1993:1999	arg1	effect					2029:2034	The greater hydroxyl radical scavenging effect	1989:2034	The greater hydroxyl radical scavenging effect of N4	1989:2040	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	5	28	theme	scavenging	820:829	arg1	effect					831:836	their radical scavenging effect	806:836	their radical scavenging effect against DPPH, hydroxyl radicals and ABTS	806:877	The polysaccharides were also analyzed by FT-IR and their radical scavenging effect against DPPH, hydroxyl radicals and ABTS was evaluated.
36687691	9	29	from	yield	1480:1484	arg1	DPPH					1585:1588	DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1585:1644	DPPH	1585:1588	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	29	from	yield	1480:1484	arg1	radicals					1637:1644	DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1585:1644	radicals	1637:1644	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	14	30	theme	polysaccharides	2358:2372	arg1	extraction					2374:2383	high-quality Noni polysaccharides extraction	2340:2383	high-quality Noni polysaccharides extraction	2340:2383	Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction.
36687691	2	31	dep	Objective	248:256	arg1	aims					275:278	aims	275:278	aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5)	275:465	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	11	32	from	content	1884:1890	arg1	radicals					1960:1967	scavenging hydroxyl radicals	1940:1967	scavenging hydroxyl radicals	1940:1967	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	32	from	content	1884:1890	arg1	DNA					1984:1986	protecting DNA	1973:1986	protecting DNA	1973:1986	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	0	33	theme	citrifolia	69:78	arg1	L					80:80	citrifolia L	69:80	Morinda citrifolia L. (Noni)	61:88	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	6	34	theme	UV-vis	898:903	arg1	assay					905:909	The UV-vis assay	894:909	The UV-vis assay	894:909	The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect.
36687691	7	35	from	effect	1047:1052	arg1	capacities					1212:1221	antioxidant capacities	1200:1221	antioxidant capacities	1200:1221	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	35	from	effect	1047:1052	arg1	yields					1092:1097	the extraction yields	1077:1097	the extraction yields	1077:1097	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	35	from	effect	1047:1052	arg1	weights					1110:1116	molecular weights	1100:1116	molecular weights	1100:1116	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	35	from	effect	1047:1052	arg1	effects					1243:1249	DNA protective effects	1228:1249	DNA protective effects of Noni polysaccharides	1228:1273	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	36	theme	uronic	1119:1124	arg1	contents					1131:1138	uronic acid contents	1119:1138	uronic acid contents	1119:1138	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	1	37	theme	citrifolia	160:169	arg1	L					171:171	citrifolia L	160:171	Introduction Morinda citrifolia L. (Noni) as an evergreen plant	139:201	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	9	38	from	effect	1575:1580	arg1	DPPH					1585:1588	DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1585:1644	DPPH	1585:1588	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	38	from	effect	1575:1580	arg1	radicals					1637:1644	DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1585:1644	radicals	1637:1644	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	0	39	from	Variation	0:8	arg1	bioactivities					44:56	bioactivities	44:56	bioactivities	44:56	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	0	39	from	Variation	0:8	arg1	properties					29:38	physicochemical properties	13:38	physicochemical properties	13:38	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	12	40	theme	radical	2010:2016	arg1	effect					2029:2034	The greater hydroxyl radical scavenging effect	1989:2034	The greater hydroxyl radical scavenging effect of N4	1989:2040	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	1	41	dep	Introduction	139:150	arg1	Noni					175:178	Noni	175:178	Noni	175:178	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	1	41	dep	Introduction	139:150	arg1	Morinda					152:158	Morinda	152:158	Morinda	152:158	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	9	42	dep	IC50	1591:1594	arg1	mg/mL					1602:1606	1.06 mg/mL	1597:1606	IC50: 1.06 mg/mL	1591:1606	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	14	43	theme	fruit	2315:2319	arg1	maturity					2321:2328	fruit maturity	2315:2328	fruit maturity aiming at high-quality Noni polysaccharides extraction	2315:2383	Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction.
36687691	9	44	dep	DPPH	1585:1588	arg1	IC50					1591:1594	IC50	1591:1594	IC50: 1.06 mg/mL	1591:1606	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	45	theme	extraction	1469:1478	arg1	%					1498:1498	8.26 ± 0.14%	1487:1498	8.26 ± 0.14%	1487:1498	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	45	theme	extraction	1469:1478	arg1	yield					1480:1484	the largest extraction yield	1457:1484	the largest extraction yield (8.26 ± 0.14%)	1457:1499	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	2	46	from	lowest	421:426	arg1	stages					405:410	stages	405:410	stages from the lowest to highest degree	405:444	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	4	47	theme	Ion	628:630	arg1	system					647:652	Ion chromatography system	628:652	Ion chromatography system	628:652	Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC.
36687691	7	48	theme	extraction	1081:1090	arg1	yields					1092:1097	the extraction yields	1077:1097	the extraction yields	1077:1097	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	7	49	theme	DNA	1228:1230	arg1	effects					1243:1249	DNA protective effects	1228:1249	DNA protective effects of Noni polysaccharides	1228:1273	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	2	50	theme	maturity	395:402	arg1	stages					385:390	five stages	380:390	five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5)	380:465	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	8	51	theme	fruit	1288:1292	arg1	stage					1303:1307	no fruit maturity stage	1285:1307	no fruit maturity stage	1285:1307	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	13	52	theme	protective	2146:2155	arg1	effects					2157:2163	the DNA protective effects	2138:2163	the DNA protective effects of N4	2138:2169	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	0	53	theme	physicochemical	13:27	arg1	properties					29:38	physicochemical properties	13:38	physicochemical properties	13:38	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	9	54	theme	±	1492:1492	arg1	%					1498:1498	8.26 ± 0.14%	1487:1498	8.26 ± 0.14%	1487:1498	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	54	theme	±	1492:1492	arg1	yield					1480:1484	the largest extraction yield	1457:1484	the largest extraction yield (8.26 ± 0.14%)	1457:1499	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	8	55	contain	had	1309:1311	arg1	stage					1303:1307	no fruit maturity stage	1285:1307	no fruit maturity stage	1285:1307	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	8	55	contain	had	1309:1311	arg2	impact					1323:1328	prominent impact	1313:1328	prominent impact	1313:1328	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	10	56	theme	ABTS	1669:1672	arg1	scavenging					1682:1691	ABTS radical scavenging	1669:1691	ABTS radical scavenging	1669:1691	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	7	57	theme	fruit	1057:1061	arg1	maturities					1063:1072	fruit maturities	1057:1072	fruit maturities	1057:1072	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	11	58	theme	protecting	1973:1982	arg1	DNA					1984:1986	protecting DNA	1973:1986	protecting DNA	1973:1986	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	14	59	theme	ideal	2299:2303	arg1	stages					2305:2310	ideal stages	2299:2310	ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction	2299:2383	Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction.
36687691	7	60	theme	antioxidant	1200:1210	arg1	capacities					1212:1221	antioxidant capacities	1200:1221	antioxidant capacities	1200:1221	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	11	61	theme	hydroxyl	1951:1958	arg1	radicals					1960:1967	scavenging hydroxyl radicals	1940:1967	scavenging hydroxyl radicals	1940:1967	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	10	62	theme	DPPH	1660:1663	arg1	activities					1693:1702	The stronger DPPH and ABTS radical scavenging activities	1647:1702	The stronger DPPH and ABTS radical scavenging activities of N5	1647:1708	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	8	63	theme	prominent	1313:1321	arg1	impact					1323:1328	prominent impact	1313:1328	prominent impact	1313:1328	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	15	64	theme	industrial	2499:2508	arg1	applications					2510:2521	various industrial applications	2491:2521	various industrial applications	2491:2521	This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
36687691	13	65	theme	N4	2168:2169	arg1	effects					2157:2163	the DNA protective effects	2138:2163	the DNA protective effects of N4	2138:2169	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	4	66	theme	monosaccharide	678:691	arg1	composition					693:703	the monosaccharide composition	674:703	the monosaccharide composition	674:703	Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC.
36687691	2	67	theme	maturation-related	299:316	arg1	changes					318:324	the maturation-related changes	295:324	the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni)	295:375	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	9	68	theme	highest	1506:1512	arg1	%					1541:1541	61.94 ± 1.86%	1529:1541	61.94 ± 1.86%	1529:1541	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	68	theme	highest	1506:1512	arg1	content					1520:1526	the highest sugar content	1502:1526	the highest sugar content (61.94 ± 1.86%)	1502:1542	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	6	69	theme	electrophoresis	919:933	arg1	assay					935:939	gel electrophoresis assay	915:939	gel electrophoresis assay	915:939	The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect.
36687691	8	70	theme	sulfuric	1337:1344	arg1	contents					1354:1361	the sulfuric radical contents	1333:1361	the sulfuric radical contents	1333:1361	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	2	71	theme	citrifolia	356:365	arg1	L					367:367	Morinda citrifolia L	348:367	Morinda citrifolia L. (Noni)	348:375	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	2	71	theme	citrifolia	356:365	arg1	Noni					371:374	Noni	371:374	Noni	371:374	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	4	72	used	used	658:661	arg2	system					647:652	Ion chromatography system	628:652	Ion chromatography system	628:652	Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC.
36687691	0	73	theme	Morinda	61:67	arg1	bioactivities					44:56	bioactivities	44:56	bioactivities	44:56	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	0	73	theme	Morinda	61:67	arg1	properties					29:38	physicochemical properties	13:38	physicochemical properties	13:38	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	13	74	theme	DNA	2142:2144	arg1	effects					2157:2163	the DNA protective effects	2138:2163	the DNA protective effects of N4	2138:2169	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	9	75	theme	61.94	1529:1533	arg1	%					1541:1541	61.94 ± 1.86%	1529:1541	61.94 ± 1.86%	1529:1541	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	75	theme	61.94	1529:1533	arg1	content					1520:1526	the highest sugar content	1502:1526	the highest sugar content (61.94 ± 1.86%)	1502:1542	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	7	76	theme	sugar	1141:1145	arg1	levels					1147:1152	sugar levels	1141:1152	sugar levels	1141:1152	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	3	77	theme	polysaccharides	567:581	arg1	Methods					468:474	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical)	468:557	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides	468:581	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	7	78	theme	Results	1005:1011	arg1	Results					1013:1019	Results Results	1005:1019	Results Results	1005:1019	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	2	79	dep	stages	385:390	arg1	stages					405:410	stages	405:410	stages from the lowest to highest degree	405:444	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	2	80	theme	present	262:268	arg1	work					270:273	The present work	258:273	The present work	258:273	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	1	81	theme	natural	223:229	arg1	polysaccharides					231:245	natural polysaccharides	223:245	natural polysaccharides	223:245	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	11	82	theme	±	1898:1898	arg1	%					1904:1904	4.10 ± 0.12%	1893:1904	4.10 ± 0.12%	1893:1904	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	82	theme	±	1898:1898	arg1	content					1884:1890	the highest uronic acid content	1860:1890	the highest uronic acid content (4.10 ± 0.12%)	1860:1905	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	83	from	performance	1925:1935	arg1	radicals					1960:1967	scavenging hydroxyl radicals	1940:1967	scavenging hydroxyl radicals	1940:1967	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	83	from	performance	1925:1935	arg1	DNA					1984:1986	protecting DNA	1973:1986	protecting DNA	1973:1986	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	84	theme	Noni	1806:1809	arg1	polysaccharides					1811:1825	Noni polysaccharides	1806:1825	Noni polysaccharides extracted at stage 4 (N4)	1806:1851	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	3	85	theme	different	601:609	arg1	assays					620:625	different chemical assays	601:625	different chemical assays	601:625	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	9	86	dep	radicals	1637:1644	arg1	IC50					1619:1622	IC50	1619:1622	IC50: 1.22 mg/mL	1619:1634	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	3	87	dep	composition	489:499	arg1	radical					550:556	sulfate radical	542:556	sulfate radical	542:556	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	3	87	dep	composition	489:499	arg1	protein					516:522	protein	516:522	protein	516:522	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	3	87	dep	composition	489:499	arg1	acid					532:535	uronic acid	525:535	uronic acid	525:535	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	3	87	dep	composition	489:499	arg1	carbohydrate					502:513	carbohydrate	502:513	carbohydrate	502:513	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	13	88	with	correlation	2192:2202	arg1	ability					2241:2247	its hydroxyl radical scavenging ability	2209:2247	its hydroxyl radical scavenging ability	2209:2247	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	14	89	dep	Conclusion	2250:2259	arg1	stages					2305:2310	ideal stages	2299:2310	ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction	2299:2383	Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction.
36687691	10	90	theme	rhamnose	1767:1774	arg1	weight					1798:1803	smaller molecular weight	1780:1803	smaller molecular weight	1780:1803	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	10	90	theme	rhamnose	1767:1774	arg1	content					1745:1751	its higher content	1734:1751	its higher content of fucose and rhamnose	1734:1774	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	9	91	theme	potent	1557:1562	arg1	effect					1575:1580	the most potent scavenging effect	1548:1580	the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1548:1644	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	12	92	theme	weight	2108:2113	arg1	counterpart					2115:2125	the low molecular weight counterpart	2090:2125	the low molecular weight counterpart	2090:2125	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	3	93	dep	Methods	468:474	arg1	composition					489:499	The chemical composition	476:499	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides	468:581	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	12	94	theme	molecular	2098:2106	arg1	weight					2108:2113	the low molecular weight	2090:2113	the low molecular weight counterpart	2090:2125	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	10	95	theme	fucose	1756:1761	arg1	weight					1798:1803	smaller molecular weight	1780:1803	smaller molecular weight	1780:1803	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	10	95	theme	fucose	1756:1761	arg1	content					1745:1751	its higher content	1734:1751	its higher content of fucose and rhamnose	1734:1774	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	0	96	theme	maturity	129:136	arg1	stages					119:124	different stages	109:124	different stages of maturity	109:136	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	8	97	theme	preliminary	1367:1377	arg1	characteristics					1389:1403	preliminary structure characteristics	1367:1403	preliminary structure characteristics	1367:1403	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	15	98	theme	suitable	2448:2455	arg1	polysaccharides					2462:2476	suitable Noni polysaccharides	2448:2476	suitable Noni polysaccharides	2448:2476	This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
36687691	13	99	theme	scavenging	2230:2239	arg1	ability					2241:2247	its hydroxyl radical scavenging ability	2209:2247	its hydroxyl radical scavenging ability	2209:2247	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	10	100	theme	smaller	1780:1786	arg1	weight					1798:1803	smaller molecular weight	1780:1803	smaller molecular weight	1780:1803	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	10	101	theme	stronger	1651:1658	arg1	DPPH					1660:1663	stronger DPPH	1651:1663	stronger DPPH	1651:1663	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	5	102	theme	radical	812:818	arg1	effect					831:836	their radical scavenging effect	806:836	their radical scavenging effect against DPPH, hydroxyl radicals and ABTS	806:877	The polysaccharides were also analyzed by FT-IR and their radical scavenging effect against DPPH, hydroxyl radicals and ABTS was evaluated.
36687691	6	103	theme	gel	915:917	arg1	assay					935:939	gel electrophoresis assay	915:939	gel electrophoresis assay	915:939	The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect.
36687691	11	104	theme	acid	1879:1882	arg1	%					1904:1904	4.10 ± 0.12%	1893:1904	4.10 ± 0.12%	1893:1904	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	11	104	theme	acid	1879:1882	arg1	content					1884:1890	the highest uronic acid content	1860:1890	the highest uronic acid content (4.10 ± 0.12%)	1860:1905	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	12	105	theme	low	2094:2096	arg1	weight					2108:2113	the low molecular weight	2090:2113	the low molecular weight counterpart	2090:2125	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	7	106	theme	acid	1126:1129	arg1	contents					1131:1138	uronic acid contents	1119:1138	uronic acid contents	1119:1138	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	14	107	theme	Noni	2353:2356	arg1	extraction					2374:2383	high-quality Noni polysaccharides extraction	2340:2383	high-quality Noni polysaccharides extraction	2340:2383	Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction.
36687691	7	108	theme	Noni	1254:1257	arg1	polysaccharides					1259:1273	Noni polysaccharides	1254:1273	Noni polysaccharides	1254:1273	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	15	109	theme	Noni	2457:2460	arg1	polysaccharides					2462:2476	suitable Noni polysaccharides	2448:2476	suitable Noni polysaccharides	2448:2476	This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
36687691	15	110	theme	polysaccharides	2462:2476	arg1	selection					2435:2443	the selection	2431:2443	the selection of suitable Noni polysaccharides to cater for various industrial applications	2431:2521	This study provided valuable information for the selection of suitable Noni polysaccharides to cater for various industrial applications.
36687691	2	111	theme	highest	431:437	arg1	degree					439:444	highest degree	431:444	highest degree	431:444	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	12	112	theme	higher	2069:2074	arg1	percentage					2076:2085	its higher percentage	2065:2085	its higher percentage of the low molecular weight counterpart	2065:2125	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	7	113	theme	molecular	1100:1108	arg1	weights					1110:1116	molecular weights	1100:1116	molecular weights	1100:1116	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	5	114	theme	hydroxyl	852:859	arg1	radicals					861:868	hydroxyl radicals	852:868	hydroxyl radicals	852:868	The polysaccharides were also analyzed by FT-IR and their radical scavenging effect against DPPH, hydroxyl radicals and ABTS was evaluated.
36687691	14	115	theme	maturity	2321:2328	arg1	stages					2305:2310	ideal stages	2299:2310	ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction	2299:2383	Conclusion Overall, stage 4 and stage 5 could be ideal stages of fruit maturity aiming at high-quality Noni polysaccharides extraction.
36687691	9	116	theme	ABTS	1613:1616	arg1	radicals					1637:1644	DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1585:1644	radicals	1637:1644	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	12	117	theme	N4	2039:2040	arg1	effect					2029:2034	The greater hydroxyl radical scavenging effect	1989:2034	The greater hydroxyl radical scavenging effect of N4	1989:2040	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	12	118	theme	hydroxyl	2001:2008	arg1	effect					2029:2034	The greater hydroxyl radical scavenging effect	1989:2034	The greater hydroxyl radical scavenging effect of N4	1989:2040	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	9	119	theme	largest	1461:1467	arg1	%					1498:1498	8.26 ± 0.14%	1487:1498	8.26 ± 0.14%	1487:1498	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	119	theme	largest	1461:1467	arg1	yield					1480:1484	the largest extraction yield	1457:1484	the largest extraction yield (8.26 ± 0.14%)	1457:1499	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	120	from	content	1520:1526	arg1	DPPH					1585:1588	DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1585:1644	DPPH	1585:1588	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	120	from	content	1520:1526	arg1	radicals					1637:1644	DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals	1585:1644	radicals	1637:1644	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	7	121	theme	protective	1232:1241	arg1	effects					1243:1249	DNA protective effects	1228:1249	DNA protective effects of Noni polysaccharides	1228:1273	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	12	122	theme	scavenging	2018:2027	arg1	effect					2029:2034	The greater hydroxyl radical scavenging effect	1989:2034	The greater hydroxyl radical scavenging effect of N4	1989:2040	The greater hydroxyl radical scavenging effect of N4 might be attributed to its higher percentage of the low molecular weight counterpart.
36687691	2	123	dep	stages	405:410	arg1	4					457:457	4	457:457	4	457:457	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	2	123	dep	stages	405:410	arg1	5					464:464	5	464:464	5	464:464	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	11	124	theme	scavenging	1940:1949	arg1	radicals					1960:1967	scavenging hydroxyl radicals	1940:1967	scavenging hydroxyl radicals	1940:1967	Noni polysaccharides extracted at stage 4 (N4) showed the highest uronic acid content (4.10 ± 0.12%), and the superior performance in scavenging hydroxyl radicals and protecting DNA.
36687691	10	125	theme	scavenging	1682:1691	arg1	activities					1693:1702	The stronger DPPH and ABTS radical scavenging activities	1647:1702	The stronger DPPH and ABTS radical scavenging activities of N5	1647:1708	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	2	126	theme	L	367:367	arg1	polysaccharides					329:343	polysaccharides	329:343	polysaccharides of Morinda citrifolia L. (Noni)	329:375	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	7	127	theme	maturities	1063:1072	arg1	effect					1047:1052	the significant effect	1031:1052	the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides	1031:1273	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	13	128	theme	positive	2183:2190	arg1	correlation					2192:2202	a positive correlation	2181:2202	a positive correlation with its hydroxyl radical scavenging ability	2181:2247	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	13	129	theme	hydroxyl	2213:2220	arg1	ability					2241:2247	its hydroxyl radical scavenging ability	2209:2247	its hydroxyl radical scavenging ability	2209:2247	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	10	130	theme	radical	1674:1680	arg1	scavenging					1682:1691	ABTS radical scavenging	1669:1691	ABTS radical scavenging	1669:1691	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	4	131	theme	chromatography	632:645	arg1	system					647:652	Ion chromatography system	628:652	Ion chromatography system	628:652	Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC.
36687691	0	132	dep	Morinda	61:67	arg1	L					80:80	citrifolia L	69:80	Morinda citrifolia L. (Noni)	61:88	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	6	133	theme	DNA	975:977	arg1	damage					979:984	DNA damage	975:984	the DNA damage protective effect	971:1002	The UV-vis assay and gel electrophoresis assay were performed to investigate the DNA damage protective effect.
36687691	3	134	theme	uronic	525:530	arg1	acid					532:535	uronic acid	525:535	uronic acid	525:535	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	8	135	theme	maturity	1294:1301	arg1	stage					1303:1307	no fruit maturity stage	1285:1307	no fruit maturity stage	1285:1307	However, no fruit maturity stage had prominent impact on the sulfuric radical contents and preliminary structure characteristics.
36687691	7	136	theme	significant	1035:1045	arg1	effect					1047:1052	the significant effect	1031:1052	the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides	1031:1273	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	10	137	theme	N5	1707:1708	arg1	activities					1693:1702	The stronger DPPH and ABTS radical scavenging activities	1647:1702	The stronger DPPH and ABTS radical scavenging activities of N5	1647:1708	The stronger DPPH and ABTS radical scavenging activities of N5 might be contributed by its higher content of fucose and rhamnose and smaller molecular weight.
36687691	4	138	theme	molecular	714:722	arg1	weight					724:729	the molecular weight	710:729	the molecular weight	710:729	Ion chromatography system was used to analyze the monosaccharide composition, and the molecular weight was measured by HPGPC.
36687691	7	139	theme	monosaccharide	1155:1168	arg1	compositions					1170:1181	monosaccharide compositions	1155:1181	monosaccharide compositions	1155:1181	Results Results indicated the significant effect of fruit maturities on the extraction yields, molecular weights, uronic acid contents, sugar levels, monosaccharide compositions and proportions, antioxidant capacities, and DNA protective effects of Noni polysaccharides.
36687691	13	140	theme	radical	2222:2228	arg1	ability					2241:2247	its hydroxyl radical scavenging ability	2209:2247	its hydroxyl radical scavenging ability	2209:2247	Moreover, the DNA protective effects of N4 displayed a positive correlation with its hydroxyl radical scavenging ability.
36687691	3	141	theme	sulfate	542:548	arg1	radical					550:556	sulfate radical	542:556	sulfate radical	542:556	Methods The chemical composition (carbohydrate, protein, uronic acid, and sulfate radical) of Noni polysaccharides was determined by different chemical assays.
36687691	1	142	theme	evergreen	187:195	arg1	plant					197:201	an evergreen plant	184:201	an evergreen plant	184:201	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	9	143	theme	sugar	1514:1518	arg1	%					1541:1541	61.94 ± 1.86%	1529:1541	61.94 ± 1.86%	1529:1541	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	9	143	theme	sugar	1514:1518	arg1	content					1520:1526	the highest sugar content	1502:1526	the highest sugar content (61.94 ± 1.86%)	1502:1542	Noni polysaccharides extracted at stage 5 (N5) had the largest extraction yield (8.26 ± 0.14%), the highest sugar content (61.94 ± 1.86%) and the most potent scavenging effect on DPPH (IC50: 1.06 mg/mL) and ABTS (IC50: 1.22 mg/mL) radicals.
36687691	0	144	from	stages	119:124	arg1	polysaccharides					90:104	polysaccharides	90:104	polysaccharides	90:104	Variation in physicochemical properties and bioactivities of Morinda citrifolia L. (Noni) polysaccharides at different stages of maturity.
36687691	2	145	theme	Morinda	348:354	arg1	L					367:367	Morinda citrifolia L	348:367	Morinda citrifolia L. (Noni)	348:375	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	2	145	theme	Morinda	348:354	arg1	Noni					371:374	Noni	371:374	Noni	371:374	Objective The present work aims to investigate the maturation-related changes in polysaccharides of Morinda citrifolia L. (Noni) at five stages of maturity (stages from the lowest to highest degree - 1, 2, 3, 4, and 5).
36687691	1	146	theme	rich	208:211	arg1	Introduction					139:150	Introduction Morinda citrifolia L. (Noni)	139:179	Introduction Morinda citrifolia L. (Noni) as an evergreen plant	139:201	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
36687691	1	146	theme	rich	208:211	arg1	source					213:218	a rich source	206:218	a rich source of natural polysaccharides	206:245	Introduction Morinda citrifolia L. (Noni) as an evergreen plant is a rich source of natural polysaccharides.
35489327	0	0	theme	microbial	89:97	arg1	composition					99:109	intestinal microbial composition	78:109	intestinal microbial composition	78:109	Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy.
35489327	5	1	theme	liver	718:722	arg1	fibrosis					724:731	liver fibrosis	718:731	liver fibrosis	718:731	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	6	2	theme	bacteria	838:845	arg1	sequencing					855:864	intestinal bacteria 16SrRNA sequencing	827:864	intestinal bacteria 16SrRNA sequencing	827:864	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	13	3	theme	anti-hepatic	1883:1894	arg1	fibrosis					1896:1903	anti-hepatic fibrosis	1883:1903	anti-hepatic fibrosis	1883:1903	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	0	4	theme	intestinal	78:87	arg1	composition					99:109	intestinal microbial composition	78:109	intestinal microbial composition	78:109	Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy.
35489327	6	5	theme	other	871:875	arg1	means					887:891	other technical means	871:891	other technical means	871:891	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	5	6	theme	high-fat	583:590	arg1	diet					592:595	acid-defined, high-fat diet	569:595	diet	592:595	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	5	6	theme	high-fat	583:590	arg1	CDAHFD					598:603	CDAHFD	598:603	CDAHFD	598:603	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	2	7	theme	liver	334:338	arg1	fibrosis					340:347	reverse liver fibrosis	326:347	reverse liver fibrosis	326:347	However, there is no effective way to reverse liver fibrosis.
35489327	1	8	theme	main	257:260	arg1	causes					262:267	the main causes	253:267	the main causes	253:267	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	6	9	theme	histopathological	742:758	arg1	staining					760:767	histopathological staining	742:767	histopathological staining	742:767	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	1	10	dep	BACKGROUND	150:159	arg1	threatening					190:200	threatening	190:200	are threatening more and more people all over the world	186:240	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	1	10	dep	BACKGROUND	150:159	arg1	fibrosis					278:285	liver fibrosis	272:285	liver fibrosis	272:285	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	1	11	theme	causes	262:267	arg1	one					246:248	one	246:248	one	246:248	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	1	11	theme	causes	262:267	arg1	causes					262:267	the main causes	253:267	the main causes	253:267	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	7	12	theme	CDAHFD-induced	1002:1015	arg1	fibrosis					1023:1030	CDAHFD-induced liver fibrosis	1002:1030	CDAHFD-induced liver fibrosis in mice	1002:1038	RESULTS G-Rh2 could notably alleviate CDAHFD-induced liver fibrosis in mice.
35489327	6	13	theme	hematoxylin-eosin	770:786	arg1	staining					794:801	hematoxylin-eosin (H&E) staining	770:801	hematoxylin-eosin (H&E) staining	770:801	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	1	14	dep	Nowadays	161:168	arg1	diseases					177:184	liver diseases	171:184	liver diseases	171:184	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	8	15	theme	lipopolysaccharide	1106:1123	arg1	levels					1131:1136	plasma lipopolysaccharide (LPS) levels	1099:1136	plasma lipopolysaccharide (LPS) levels	1099:1136	In particular, it could alleviate liver injury and reduce plasma lipopolysaccharide (LPS) levels.
35489327	0	16	theme	LPS-mediated	126:137	arg1	autophagy					139:147	LPS-mediated autophagy	126:147	LPS-mediated autophagy	126:147	Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy.
35489327	9	17	theme	intestinal	1172:1181	arg1	injury					1183:1188	intestinal injury	1172:1188	intestinal injury	1172:1188	Additionally, G-Rh2 could repair intestinal injury as well as regulate intestinal microbial diversity and composition.
35489327	5	18	dep	Choline-deficient	542:558	arg1	L-amino					561:567	L-amino	561:567	L-amino	561:567	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	3	19	theme	liver	419:423	arg1	fibrosis					425:432	liver fibrosis	419:432	liver fibrosis	419:432	PURPOSE To investigate whether ginsenoside Rh2 (G-Rh2) can alleviate liver fibrosis and elucidate its underlying mechanism.
35489327	5	20	theme	LPS-induced	697:707	arg1	model					709:713	an LPS-induced model	694:713	an LPS-induced model of liver fibrosis	694:731	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	0	21	from	fibrosis	48:55	arg1	mice					60:63	mice	60:63	mice	60:63	Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy.
35489327	13	22	theme	microbial	1718:1726	arg1	composition					1728:1738	intestinal microbial composition	1707:1738	intestinal microbial composition	1707:1738	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	0	23	theme	Ginsenoside	0:10	arg1	Rh2					12:14	Ginsenoside Rh2	0:14	Ginsenoside Rh2	0:14	Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy.
35489327	13	24	theme	important	1865:1873	arg1	role					1875:1878	an important role	1862:1878	an important role	1862:1878	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	13	25	dep	CONCLUSION	1606:1615	arg1	suggest					1652:1658	suggest	1652:1658	suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis	1652:1903	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	13	26	theme	plasma	1749:1754	arg1	levels					1760:1765	plasma LPS levels	1749:1765	plasma LPS levels	1749:1765	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	5	27	theme	fibrosis	724:731	arg1	model					709:713	an LPS-induced model	694:713	an LPS-induced model of liver fibrosis	694:731	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	11	28	theme	stellate	1429:1436	arg1	cells					1438:1442	hepatic stellate cells	1421:1442	hepatic stellate cells (HSCs)	1421:1449	After being treated with G-Rh2, autophagy was restrained and activation of hepatic stellate cells (HSCs) was controlled.
35489327	11	28	theme	stellate	1429:1436	arg1	HSCs					1445:1448	HSCs	1445:1448	HSCs	1445:1448	After being treated with G-Rh2, autophagy was restrained and activation of hepatic stellate cells (HSCs) was controlled.
35489327	6	29	theme	anti-fibrotic	940:952	arg1	activity					954:961	anti-fibrotic activity	940:961	anti-fibrotic activity	940:961	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	13	30	theme	AKT-mTOR	1786:1793	arg1	pathway					1805:1811	the AKT-mTOR signaling pathway	1782:1811	the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy	1782:1846	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	3	31	theme	ginsenoside	381:391	arg1	G-Rh2					398:402	G-Rh2	398:402	G-Rh2	398:402	PURPOSE To investigate whether ginsenoside Rh2 (G-Rh2) can alleviate liver fibrosis and elucidate its underlying mechanism.
35489327	3	31	theme	ginsenoside	381:391	arg1	Rh2					393:395	ginsenoside Rh2	381:395	ginsenoside Rh2 (G-Rh2)	381:403	PURPOSE To investigate whether ginsenoside Rh2 (G-Rh2) can alleviate liver fibrosis and elucidate its underlying mechanism.
35489327	1	32	theme	liver	272:276	arg1	fibrosis					278:285	liver fibrosis	272:285	liver fibrosis	272:285	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	13	33	theme	intestinal	1679:1688	arg1	injury					1690:1695	intestinal injury	1679:1695	intestinal injury	1679:1695	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	5	34	theme	acid-defined	569:580	arg1	diet					592:595	acid-defined, high-fat diet	569:595	diet	592:595	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	5	34	theme	acid-defined	569:580	arg1	CDAHFD					598:603	CDAHFD	598:603	CDAHFD	598:603	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	14	35	contain	have	1925:1928	arg1	G-Rh2					1906:1910	G-Rh2	1906:1910	G-Rh2	1906:1910	G-Rh2 was found to have the potential to effectively alleviate liver fibrosis.
35489327	14	35	contain	have	1925:1928	arg2	potential					1934:1942	the potential to effectively alleviate liver fibrosis	1930:1982	the potential to effectively alleviate liver fibrosis	1930:1982	G-Rh2 was found to have the potential to effectively alleviate liver fibrosis.
35489327	13	36	theme	LPS-mediated	1825:1836	arg1	autophagy					1838:1846	LPS-mediated autophagy	1825:1846	LPS-mediated autophagy	1825:1846	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	12	37	theme	Deeper	1467:1472	arg1	research					1474:1481	Deeper research	1467:1481	Deeper research	1467:1481	Deeper research showed that G-Rh2 restrained the activation of HSCs via stimulating the AKT-mTOR signaling pathway, restraining autophagy.
35489327	0	38	theme	CDAHFD-induced	27:40	arg1	fibrosis					48:55	CDAHFD-induced liver fibrosis	27:55	CDAHFD-induced liver fibrosis in mice	27:63	Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy.
35489327	4	39	dep	METHODS	474:480	arg1	vivo					485:488	In vivo	482:488	In vivo	482:488	METHODS In vivo and in vitro methods were adopted in this research.
35489327	4	39	dep	METHODS	474:480	arg1	vitro					497:501	in vitro	494:501	in vitro	494:501	METHODS In vivo and in vitro methods were adopted in this research.
35489327	5	40	theme	HSC-T6	658:663	arg1	cells					665:669	HSC-T6 cells	658:669	HSC-T6 cells	658:669	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	6	41	theme	H&E	789:791	arg1	staining					794:801	hematoxylin-eosin (H&E) staining	770:801	hematoxylin-eosin (H&E) staining	770:801	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	1	42	theme	people	216:221	arg1	all					223:225	people all	216:225	people all over the world	216:240	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	13	43	theme	studies	1636:1642	arg1	results					1621:1627	The results	1617:1627	The results of our studies	1617:1642	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	6	44	theme	intestinal	827:836	arg1	sequencing					855:864	intestinal bacteria 16SrRNA sequencing	827:864	intestinal bacteria 16SrRNA sequencing	827:864	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	6	45	theme	technical	877:885	arg1	means					887:891	other technical means	871:891	other technical means	871:891	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	3	46	theme	underlying	452:461	arg1	mechanism					463:471	its underlying mechanism	448:471	its underlying mechanism	448:471	PURPOSE To investigate whether ginsenoside Rh2 (G-Rh2) can alleviate liver fibrosis and elucidate its underlying mechanism.
35489327	5	47	used	used	676:679	arg2	cells					665:669	HSC-T6 cells	658:669	HSC-T6 cells	658:669	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	11	48	theme	hepatic	1421:1427	arg1	cells					1438:1442	hepatic stellate cells	1421:1442	hepatic stellate cells (HSCs)	1421:1449	After being treated with G-Rh2, autophagy was restrained and activation of hepatic stellate cells (HSCs) was controlled.
35489327	11	48	theme	hepatic	1421:1427	arg1	HSCs					1445:1448	HSCs	1445:1448	HSCs	1445:1448	After being treated with G-Rh2, autophagy was restrained and activation of hepatic stellate cells (HSCs) was controlled.
35489327	8	49	theme	liver	1075:1079	arg1	injury					1081:1086	liver injury	1075:1086	liver injury	1075:1086	In particular, it could alleviate liver injury and reduce plasma lipopolysaccharide (LPS) levels.
35489327	9	50	theme	intestinal	1210:1219	arg1	diversity					1231:1239	intestinal microbial diversity	1210:1239	intestinal microbial diversity	1210:1239	Additionally, G-Rh2 could repair intestinal injury as well as regulate intestinal microbial diversity and composition.
35489327	11	51	theme	cells	1438:1442	arg1	activation					1407:1416	activation	1407:1416	activation of hepatic stellate cells (HSCs)	1407:1449	After being treated with G-Rh2, autophagy was restrained and activation of hepatic stellate cells (HSCs) was controlled.
35489327	0	52	theme	liver	42:46	arg1	fibrosis					48:55	CDAHFD-induced liver fibrosis	27:55	CDAHFD-induced liver fibrosis in mice	27:63	Ginsenoside Rh2 attenuates CDAHFD-induced liver fibrosis in mice by improving intestinal microbial composition and regulating LPS-mediated autophagy.
35489327	12	53	theme	AKT-mTOR	1555:1562	arg1	pathway					1574:1580	the AKT-mTOR signaling pathway	1551:1580	the AKT-mTOR signaling pathway	1551:1580	Deeper research showed that G-Rh2 restrained the activation of HSCs via stimulating the AKT-mTOR signaling pathway, restraining autophagy.
35489327	13	54	dep	pathway	1805:1811	arg1	restrain					1816:1823	restrain	1816:1823	to restrain LPS-mediated autophagy	1813:1846	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	9	55	theme	microbial	1221:1229	arg1	diversity					1231:1239	intestinal microbial diversity	1210:1239	intestinal microbial diversity	1210:1239	Additionally, G-Rh2 could repair intestinal injury as well as regulate intestinal microbial diversity and composition.
35489327	14	56	theme	liver	1969:1973	arg1	fibrosis					1975:1982	liver fibrosis	1969:1982	liver fibrosis	1969:1982	G-Rh2 was found to have the potential to effectively alleviate liver fibrosis.
35489327	12	57	theme	signaling	1564:1572	arg1	pathway					1574:1580	the AKT-mTOR signaling pathway	1551:1580	the AKT-mTOR signaling pathway	1551:1580	Deeper research showed that G-Rh2 restrained the activation of HSCs via stimulating the AKT-mTOR signaling pathway, restraining autophagy.
35489327	5	58	theme	liver	638:642	arg1	fibrosis					644:651	liver fibrosis	638:651	liver fibrosis	638:651	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	6	59	theme	blot	812:815	arg1	analysis					817:824	western blot analysis	804:824	western blot analysis	804:824	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	6	60	contain	possesses	930:938	arg1	G-Rh2					924:928	G-Rh2	924:928	G-Rh2	924:928	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	6	60	contain	possesses	930:938	arg2	activity					954:961	anti-fibrotic activity	940:961	anti-fibrotic activity	940:961	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	6	61	theme	16SrRNA	847:853	arg1	sequencing					855:864	intestinal bacteria 16SrRNA sequencing	827:864	intestinal bacteria 16SrRNA sequencing	827:864	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	2	62	theme	reverse	326:332	arg1	fibrosis					340:347	reverse liver fibrosis	326:347	reverse liver fibrosis	326:347	However, there is no effective way to reverse liver fibrosis.
35489327	6	63	theme	western	804:810	arg1	analysis					817:824	western blot analysis	804:824	western blot analysis	804:824	Through histopathological staining, hematoxylin-eosin (H&E) staining, western blot analysis, intestinal bacteria 16SrRNA sequencing, and other technical means, the research explored whether G-Rh2 possesses anti-fibrotic activity.
35489327	13	64	theme	intestinal	1707:1716	arg1	composition					1728:1738	intestinal microbial composition	1707:1738	intestinal microbial composition	1707:1738	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	4	65	dep	vivo	485:488	arg1	methods					503:509	methods	503:509	methods	503:509	METHODS In vivo and in vitro methods were adopted in this research.
35489327	7	66	theme	liver	1017:1021	arg1	fibrosis					1023:1030	CDAHFD-induced liver fibrosis	1002:1030	CDAHFD-induced liver fibrosis in mice	1002:1038	RESULTS G-Rh2 could notably alleviate CDAHFD-induced liver fibrosis in mice.
35489327	13	67	theme	LPS	1756:1758	arg1	levels					1760:1765	plasma LPS levels	1749:1765	plasma LPS levels	1749:1765	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	8	68	theme	plasma	1099:1104	arg1	LPS					1126:1128	LPS	1126:1128	LPS	1126:1128	In particular, it could alleviate liver injury and reduce plasma lipopolysaccharide (LPS) levels.
35489327	8	68	theme	plasma	1099:1104	arg1	lipopolysaccharide					1106:1123	plasma lipopolysaccharide	1099:1123	plasma lipopolysaccharide (LPS) levels	1099:1136	In particular, it could alleviate liver injury and reduce plasma lipopolysaccharide (LPS) levels.
35489327	5	69	theme	Choline-deficient	542:558	arg1	diet					592:595	acid-defined, high-fat diet	569:595	diet	592:595	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	5	69	theme	Choline-deficient	542:558	arg1	CDAHFD					598:603	CDAHFD	598:603	CDAHFD	598:603	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	12	70	theme	HSCs	1530:1533	arg1	activation					1516:1525	the activation	1512:1525	the activation of HSCs	1512:1533	Deeper research showed that G-Rh2 restrained the activation of HSCs via stimulating the AKT-mTOR signaling pathway, restraining autophagy.
35489327	7	71	theme	RESULTS	964:970	arg1	G-Rh2					972:976	RESULTS G-Rh2	964:976	RESULTS G-Rh2	964:976	RESULTS G-Rh2 could notably alleviate CDAHFD-induced liver fibrosis in mice.
35489327	2	72	theme	effective	309:317	arg1	way					319:321	no effective way	306:321	no effective way to reverse liver fibrosis	306:347	However, there is no effective way to reverse liver fibrosis.
35489327	7	73	from	fibrosis	1023:1030	arg1	mice					1035:1038	mice	1035:1038	mice	1035:1038	RESULTS G-Rh2 could notably alleviate CDAHFD-induced liver fibrosis in mice.
35489327	13	74	theme	signaling	1795:1803	arg1	pathway					1805:1811	the AKT-mTOR signaling pathway	1782:1811	the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy	1782:1846	CONCLUSION The results of our studies clearly suggest that G-Rh2 repairs intestinal injury, improves intestinal microbial composition, reduces plasma LPS levels, and activates the AKT-mTOR signaling pathway to restrain LPS-mediated autophagy, thus playing an important role in anti-hepatic fibrosis.
35489327	5	75	used	used	610:613	arg2	CDAHFD					598:603	CDAHFD	598:603	CDAHFD	598:603	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	5	75	used	used	610:613	arg2	diet					592:595	acid-defined, high-fat diet	569:595	diet	592:595	Choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) was used to feed mice to induce liver fibrosis, and HSC-T6 cells were used to establish an LPS-induced model of liver fibrosis.
35489327	1	76	theme	liver	171:175	arg1	diseases					177:184	liver diseases	171:184	liver diseases	171:184	BACKGROUND Nowadays, liver diseases are threatening more and more people all over the world and one of the main causes is liver fibrosis.
35489327	10	77	theme	HSC-T6	1258:1263	arg1	cells					1265:1269	HSC-T6 cells	1258:1269	HSC-T6 cells	1258:1269	HSC-T6 cells could be activated and autophagy could be induced further by LPS in vitro.
37404852	14	0	theme	bone	2432:2435	arg1	defects					2437:2443	large bone defects	2426:2443	large bone defects	2426:2443	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	6	1	with	experiments	1265:1275	arg1	BMSCs					1297:1301	BMSCs	1297:1301	BMSCs	1297:1301	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	4	2	theme	biomimetic	839:848	arg1	scaffolds					850:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds	745:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration	745:890	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	7	3	theme	differentiation	1466:1480	arg1	mechanism					1482:1490	the osteogenic differentiation mechanism	1451:1490	the osteogenic differentiation mechanism	1451:1490	Transcriptomic sequencing was performed to analyze the osteogenic differentiation mechanism.
37404852	2	4	theme	composite	287:295	arg1	materials					297:305	composite materials	287:305	composite materials that mimic the complex structure and biological activity of natural bone	287:378	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	5	5	theme	cumulative	1207:1216	arg1	curve					1226:1230	the cumulative release curve	1203:1230	the cumulative release curve of rhCXCL13	1203:1242	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	4	6	theme	ligand	811:816	arg1	scaffolds					850:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds	745:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration	745:890	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	12	7	theme	experimental	1894:1905	arg1	results					1907:1913	Transcriptome sequencing and experimental results	1865:1913	results	1907:1913	Transcriptome sequencing and experimental results showed that the osteogenesis mechanism of rhCXCL13-HHM/CS was through the PI3K-AKT pathway.
37404852	6	8	theme	migration	1255:1263	arg1	experiments					1265:1275	Transwell migration experiments	1245:1275	Transwell migration experiments	1245:1275	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	3	9	theme	Hollow	535:540	arg1	HHMs					571:574	HHMs	571:574	HHMs	571:574	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	9	theme	Hollow	535:540	arg1	microspheres					557:568	Hollow hydroxyapatite microspheres	535:568	Hollow hydroxyapatite microspheres (HHMs)	535:575	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	14	10	theme	promising	2381:2389	arg1	applications					2400:2411	material osteogenesis mechanism study and promising clinical applications	2339:2411	applications	2400:2411	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	0	11	theme	Bone	128:131	arg1	Regeneration					133:144	Bone Regeneration	128:144	Bone Regeneration	128:144	Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects.
37404852	2	12	from	application	477:487	arg1	regeneration					521:532	situ bone regeneration	511:532	situ bone regeneration	511:532	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	7	13	theme	Transcriptomic	1400:1413	arg1	sequencing					1415:1424	Transcriptomic sequencing	1400:1424	Transcriptomic sequencing	1400:1424	Transcriptomic sequencing was performed to analyze the osteogenic differentiation mechanism.
37404852	14	14	theme	osteogenesis	2348:2359	arg1	study					2371:2375	material osteogenesis mechanism study and promising clinical applications	2339:2411	study	2371:2375	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	5	15	theme	HHM/CS	1082:1087	arg1	characteristics					1059:1073	the physical characteristics	1046:1073	the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds	1046:1128	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	3	16	theme	bone	602:605	arg1	structure					607:615	a natural porous bone structure	585:615	a natural porous bone structure	585:615	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	8	17	theme	osteogenesis	1497:1508	arg1	performance					1527:1537	The osteogenesis and bone healing performance	1493:1537	The osteogenesis and bone healing performance	1493:1537	The osteogenesis and bone healing performance were evaluated using a rabbit radial defect model.
37404852	3	18	theme	natural	587:593	arg1	structure					607:615	a natural porous bone structure	585:615	a natural porous bone structure	585:615	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	4	19	theme	bone	874:877	arg1	regeneration					879:890	bone regeneration	874:890	bone regeneration	874:890	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	8	20	theme	bone	1514:1517	arg1	performance					1527:1537	The osteogenesis and bone healing performance	1493:1537	The osteogenesis and bone healing performance	1493:1537	The osteogenesis and bone healing performance were evaluated using a rabbit radial defect model.
37404852	5	21	theme	physical	1050:1057	arg1	characteristics					1059:1073	the physical characteristics	1046:1073	the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds	1046:1128	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	2	22	theme	current	258:264	arg1	methods					266:272	the current methods	254:272	the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone	254:378	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	4	23	theme	osteogenesis	949:960	arg1	mechanism					915:923	their mechanism	909:923	their mechanism of BMSC recruitment and osteogenesis	909:960	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	11	24	theme	bone	1846:1849	arg1	regeneration					1851:1862	bone regeneration	1846:1862	bone regeneration	1846:1862	The rhCXCL13-HHM/CS scaffold could recruit BMSCs and induce bone regeneration.
37404852	5	25	theme	Scanning	1138:1145	arg1	SEM					1168:1170	SEM	1168:1170	SEM	1168:1170	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	5	25	theme	Scanning	1138:1145	arg1	Microscopy					1156:1165	Scanning Electron Microscopy	1138:1165	Scanning Electron Microscopy (SEM)	1138:1171	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	14	26	dep	Conclusion	2125:2134	arg1	demonstrates					2165:2176	demonstrates	2165:2176	demonstrates	2165:2176	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	4	27	theme	cell	970:973	arg1	experiments					986:996	cell and animal experiments	970:996	experiments	986:996	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	13	28	theme	rhCXCL13-HHM/CS	2020:2034	arg1	scaffold					2036:2043	the rhCXCL13-HHM/CS scaffold	2016:2043	the rhCXCL13-HHM/CS scaffold	2016:2043	In vivo, the rhCXCL13-HHM/CS scaffold significantly promoted osteogenesis and angiogenesis at 12 weeks after surgery.
37404852	6	29	theme	scaffolds	1389:1397	arg1	ability					1343:1349	recruitment ability	1331:1349	recruitment ability	1331:1349	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	6	29	theme	scaffolds	1389:1397	arg1	differentiation					1366:1380	osteogenic differentiation	1355:1380	osteogenic differentiation	1355:1380	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	1	30	theme	Bone	176:179	arg1	method					215:220	a promising method	203:220	a promising method to treat bone defects	203:242	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	1	30	theme	Bone	176:179	arg1	engineering					188:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	0	31	theme	Synergetic	93:102	arg1	Angiogenesis					104:115	Synergetic Angiogenesis	93:115	Synergetic Angiogenesis	93:115	Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects.
37404852	5	32	theme	rhCXCL13-HHM/CS	1093:1107	arg1	scaffolds					1120:1128	the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds	1078:1128	scaffolds	1120:1128	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	14	33	theme	BMSC	2202:2205	arg1	recruitment					2207:2217	BMSC recruitment	2202:2217	BMSC recruitment	2202:2217	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	2	34	theme	situ	511:514	arg1	regeneration					521:532	situ bone regeneration	511:532	situ bone regeneration	511:532	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	8	35	theme	radial	1569:1574	arg1	model					1583:1587	a rabbit radial defect model	1560:1587	a rabbit radial defect model	1560:1587	The osteogenesis and bone healing performance were evaluated using a rabbit radial defect model.
37404852	4	36	theme	HHM/chitosan	749:760	arg1	scaffolds					850:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds	745:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration	745:890	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	10	37	theme	excellent	1746:1754	arg1	capability					1774:1783	excellent sustained release capability	1746:1783	excellent sustained release capability	1746:1783	The rhCXCL13 showed excellent sustained release capability.
37404852	6	38	theme	osteogenic	1355:1364	arg1	differentiation					1366:1380	osteogenic differentiation	1355:1380	osteogenic differentiation	1355:1380	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	5	39	theme	scaffolds	1120:1128	arg1	characteristics					1059:1073	the physical characteristics	1046:1073	the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds	1046:1128	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	14	40	theme	drug	2290:2293	arg1	delivery					2295:2302	drug delivery	2290:2302	drug delivery	2290:2302	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	2	41	theme	materials	498:506	arg1	application					477:487	the application	473:487	the application of these materials in situ bone regeneration	473:532	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	0	42	theme	Hollow	0:5	arg1	Microspheres					22:33	Hollow Hydroxyapatite Microspheres	0:33	Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13	0:54	Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects.
37404852	9	43	theme	rhCXCL13-HHM/CS	1624:1638	arg1	scaffold					1640:1647	the rhCXCL13-HHM/CS scaffold	1620:1647	the rhCXCL13-HHM/CS scaffold	1620:1647	Results SEM demonstrated that the rhCXCL13-HHM/CS scaffold comprised hydroxyapatite microspheres in a porous three-dimensional network.
37404852	2	44	theme	stem	439:442	arg1	BMSCs					451:455	BMSCs	451:455	BMSCs	451:455	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	2	44	theme	stem	439:442	arg1	cells					444:448	bone marrow mesenchymal stem cells	415:448	bone marrow mesenchymal stem cells (BMSCs)	415:456	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	14	45	theme	rhCXCL13-HHM/CS	2140:2154	arg1	scaffold					2156:2163	The rhCXCL13-HHM/CS scaffold	2136:2163	The rhCXCL13-HHM/CS scaffold	2136:2163	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	4	46	theme	animal	979:984	arg1	experiments					986:996	cell and animal experiments	970:996	experiments	986:996	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	14	47	theme	bone	2265:2268	arg1	reconstruction					2270:2283	vascularized tissue-engineered bone reconstruction	2234:2283	vascularized tissue-engineered bone reconstruction	2234:2283	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	10	48	theme	release	1766:1772	arg1	capability					1774:1783	excellent sustained release capability	1746:1783	excellent sustained release capability	1746:1783	The rhCXCL13 showed excellent sustained release capability.
37404852	4	49	theme	human	783:787	arg1	chemokine					801:809	recombinant human C-X-C motif chemokine	771:809	recombinant human C-X-C motif chemokine	771:809	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	2	50	theme	marrow	420:425	arg1	BMSCs					451:455	BMSCs	451:455	BMSCs	451:455	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	2	50	theme	marrow	420:425	arg1	cells					444:448	bone marrow mesenchymal stem cells	415:448	bone marrow mesenchymal stem cells (BMSCs)	415:456	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	14	51	theme	vascularized	2234:2245	arg1	reconstruction					2270:2283	vascularized tissue-engineered bone reconstruction	2234:2283	vascularized tissue-engineered bone reconstruction	2234:2283	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	11	52	theme	rhCXCL13-HHM/CS	1790:1804	arg1	scaffold					1806:1813	The rhCXCL13-HHM/CS scaffold	1786:1813	The rhCXCL13-HHM/CS scaffold	1786:1813	The rhCXCL13-HHM/CS scaffold could recruit BMSCs and induce bone regeneration.
37404852	4	53	theme	motif	795:799	arg1	chemokine					801:809	recombinant human C-X-C motif chemokine	771:809	recombinant human C-X-C motif chemokine	771:809	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	5	54	theme	release	1218:1224	arg1	curve					1226:1230	the cumulative release curve	1203:1230	the cumulative release curve of rhCXCL13	1203:1242	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	12	55	theme	PI3K-AKT	1989:1996	arg1	pathway					1998:2004	the PI3K-AKT pathway	1985:2004	the PI3K-AKT pathway	1985:2004	Transcriptome sequencing and experimental results showed that the osteogenesis mechanism of rhCXCL13-HHM/CS was through the PI3K-AKT pathway.
37404852	9	56	theme	three-dimensional	1699:1715	arg1	network					1717:1723	a porous three-dimensional network	1690:1723	a porous three-dimensional network	1690:1723	Results SEM demonstrated that the rhCXCL13-HHM/CS scaffold comprised hydroxyapatite microspheres in a porous three-dimensional network.
37404852	3	57	contain	have	671:674	arg1	HHMs					571:574	HHMs	571:574	HHMs	571:574	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	57	contain	have	671:674	arg1	microspheres					557:568	Hollow hydroxyapatite microspheres	535:568	Hollow hydroxyapatite microspheres (HHMs)	535:575	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	57	contain	have	671:674	arg2	ability					680:686	low ability	676:686	low ability to recruit BMSCs and induce osteogenesis	676:727	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	58	theme	chemokines	655:664	arg1	release					644:650	slow release	639:650	slow release of chemokines	639:664	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	58	theme	chemokines	655:664	arg1	structure					607:615	a natural porous bone structure	585:615	a natural porous bone structure	585:615	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	58	theme	chemokines	655:664	arg1	adsorption					623:632	good adsorption	618:632	good adsorption	618:632	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	12	59	theme	osteogenesis	1931:1942	arg1	mechanism					1944:1952	the osteogenesis mechanism	1927:1952	the osteogenesis mechanism of rhCXCL13-HHM/CS	1927:1971	Transcriptome sequencing and experimental results showed that the osteogenesis mechanism of rhCXCL13-HHM/CS was through the PI3K-AKT pathway.
37404852	2	60	theme	natural	367:373	arg1	bone					375:378	natural bone	367:378	natural bone	367:378	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	14	61	theme	large	2426:2430	arg1	defects					2437:2443	large bone defects	2426:2443	large bone defects	2426:2443	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	3	62	theme	low	676:678	arg1	ability					680:686	low ability	676:686	low ability to recruit BMSCs and induce osteogenesis	676:727	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	63	theme	hydroxyapatite	542:555	arg1	HHMs					571:574	HHMs	571:574	HHMs	571:574	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	63	theme	hydroxyapatite	542:555	arg1	microspheres					557:568	Hollow hydroxyapatite microspheres	535:568	Hollow hydroxyapatite microspheres (HHMs)	535:575	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	4	64	theme	-HHM/CS	831:837	arg1	scaffolds					850:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds	745:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration	745:890	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	1	65	theme	tissue	181:186	arg1	method					215:220	a promising method	203:220	a promising method to treat bone defects	203:242	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	1	65	theme	tissue	181:186	arg1	engineering					188:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	4	66	theme	chemokine	801:809	arg1	scaffolds					850:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds	745:858	The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration	745:890	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	7	67	theme	osteogenic	1455:1464	arg1	mechanism					1482:1490	the osteogenic differentiation mechanism	1451:1490	the osteogenic differentiation mechanism	1451:1490	Transcriptomic sequencing was performed to analyze the osteogenic differentiation mechanism.
37404852	14	68	theme	clinical	2391:2398	arg1	applications					2400:2411	material osteogenesis mechanism study and promising clinical applications	2339:2411	applications	2400:2411	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	6	69	theme	Transwell	1245:1253	arg1	experiments					1265:1275	Transwell migration experiments	1245:1275	Transwell migration experiments	1245:1275	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	2	70	theme	complex	322:328	arg1	structure					330:338	complex structure	322:338	complex structure	322:338	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	12	71	theme	Transcriptome	1865:1877	arg1	sequencing					1879:1888	Transcriptome sequencing and experimental results	1865:1913	sequencing	1879:1888	Transcriptome sequencing and experimental results showed that the osteogenesis mechanism of rhCXCL13-HHM/CS was through the PI3K-AKT pathway.
37404852	1	72	theme	promising	205:213	arg1	method					215:220	a promising method	203:220	a promising method to treat bone defects	203:242	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	1	72	theme	promising	205:213	arg1	engineering					188:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	14	73	theme	mechanism	2361:2369	arg1	study					2371:2375	material osteogenesis mechanism study and promising clinical applications	2339:2411	study	2371:2375	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	0	74	theme	Bone	149:152	arg1	Defects					154:160	Bone Defects	149:160	Bone Defects	149:160	Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects.
37404852	3	75	theme	porous	595:600	arg1	structure					607:615	a natural porous bone structure	585:615	a natural porous bone structure	585:615	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	6	76	theme	recruitment	1331:1341	arg1	ability					1343:1349	recruitment ability	1331:1349	recruitment ability	1331:1349	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	14	77	theme	material	2339:2346	arg1	study					2371:2375	material osteogenesis mechanism study and promising clinical applications	2339:2411	study	2371:2375	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	6	78	dep	ability	1343:1349	arg1	the					1327:1329	the	1327:1329	the	1327:1329	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	8	79	theme	healing	1519:1525	arg1	performance					1527:1537	The osteogenesis and bone healing performance	1493:1537	The osteogenesis and bone healing performance	1493:1537	The osteogenesis and bone healing performance were evaluated using a rabbit radial defect model.
37404852	3	80	theme	good	618:621	arg1	adsorption					623:632	good adsorption	618:632	good adsorption	618:632	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	1	81	theme	bone	231:234	arg1	defects					236:242	bone defects	231:242	bone defects	231:242	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	5	82	theme	Electron	1147:1154	arg1	SEM					1168:1170	SEM	1168:1170	SEM	1168:1170	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	5	82	theme	Electron	1147:1154	arg1	Microscopy					1156:1165	Scanning Electron Microscopy	1138:1165	Scanning Electron Microscopy (SEM)	1138:1171	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	1	83	theme	Introduction	163:174	arg1	method					215:220	a promising method	203:220	a promising method to treat bone defects	203:242	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	1	83	theme	Introduction	163:174	arg1	engineering					188:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering	163:198	Introduction Bone tissue engineering is a promising method to treat bone defects.
37404852	8	84	theme	rabbit	1562:1567	arg1	model					1583:1587	a rabbit radial defect model	1560:1587	a rabbit radial defect model	1560:1587	The osteogenesis and bone healing performance were evaluated using a rabbit radial defect model.
37404852	14	85	theme	theoretical	2317:2327	arg1	basis					2329:2333	a theoretical basis	2315:2333	a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects	2315:2443	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	9	86	theme	Results	1590:1596	arg1	SEM					1598:1600	Results SEM	1590:1600	Results SEM	1590:1600	Results SEM demonstrated that the rhCXCL13-HHM/CS scaffold comprised hydroxyapatite microspheres in a porous three-dimensional network.
37404852	2	87	theme	bone	516:519	arg1	regeneration					521:532	situ bone regeneration	511:532	situ bone regeneration	511:532	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	8	88	theme	defect	1576:1581	arg1	model					1583:1587	a rabbit radial defect model	1560:1587	a rabbit radial defect model	1560:1587	The osteogenesis and bone healing performance were evaluated using a rabbit radial defect model.
37404852	5	89	theme	biomimetic	1109:1118	arg1	scaffolds					1120:1128	the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds	1078:1128	scaffolds	1120:1128	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	4	90	theme	BMSC	928:931	arg1	recruitment					933:943	BMSC recruitment	928:943	BMSC recruitment	928:943	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	4	91	theme	recruitment	933:943	arg1	mechanism					915:923	their mechanism	909:923	their mechanism of BMSC recruitment and osteogenesis	909:960	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	0	92	theme	Hydroxyapatite	7:20	arg1	Microspheres					22:33	Hollow Hydroxyapatite Microspheres	0:33	Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13	0:54	Hollow Hydroxyapatite Microspheres Loaded with rhCXCL13 to Recruit BMSC for Osteogenesis and Synergetic Angiogenesis to Promote Bone Regeneration in Bone Defects.
37404852	14	93	theme	excellent	2178:2186	arg1	potential					2188:2196	excellent potential	2178:2196	excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery	2178:2302	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	4	94	theme	transcriptomic	1002:1015	arg1	sequencing					1017:1026	transcriptomic sequencing	1002:1026	transcriptomic sequencing	1002:1026	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	2	95	dep	structure	330:338	arg1	the					318:320	the	318:320	the	318:320	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	10	96	theme	sustained	1756:1764	arg1	capability					1774:1783	excellent sustained release capability	1746:1783	excellent sustained release capability	1746:1783	The rhCXCL13 showed excellent sustained release capability.
37404852	2	97	theme	cells	444:448	arg1	recruitment					400:410	recruitment	400:410	recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration	400:532	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	3	98	contain	possess	577:583	arg2	adsorption					623:632	good adsorption	618:632	good adsorption	618:632	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	98	contain	possess	577:583	arg1	HHMs					571:574	HHMs	571:574	HHMs	571:574	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	98	contain	possess	577:583	arg1	microspheres					557:568	Hollow hydroxyapatite microspheres	535:568	Hollow hydroxyapatite microspheres (HHMs)	535:575	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	98	contain	possess	577:583	arg2	release					644:650	slow release	639:650	slow release of chemokines	639:664	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	98	contain	possess	577:583	arg2	structure					607:615	a natural porous bone structure	585:615	a natural porous bone structure	585:615	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	3	99	theme	slow	639:642	arg1	release					644:650	slow release	639:650	slow release of chemokines	639:664	Hollow hydroxyapatite microspheres (HHMs) possess a natural porous bone structure, good adsorption, and slow release of chemokines, but have low ability to recruit BMSCs and induce osteogenesis.
37404852	2	100	theme	mesenchymal	427:437	arg1	BMSCs					451:455	BMSCs	451:455	BMSCs	451:455	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	2	100	theme	mesenchymal	427:437	arg1	cells					444:448	bone marrow mesenchymal stem cells	415:448	bone marrow mesenchymal stem cells (BMSCs)	415:456	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	9	101	theme	hydroxyapatite	1659:1672	arg1	microspheres					1674:1685	hydroxyapatite microspheres	1659:1685	hydroxyapatite microspheres in a porous three-dimensional network	1659:1723	Results SEM demonstrated that the rhCXCL13-HHM/CS scaffold comprised hydroxyapatite microspheres in a porous three-dimensional network.
37404852	9	102	from	microspheres	1674:1685	arg1	network					1717:1723	a porous three-dimensional network	1690:1723	a porous three-dimensional network	1690:1723	Results SEM demonstrated that the rhCXCL13-HHM/CS scaffold comprised hydroxyapatite microspheres in a porous three-dimensional network.
37404852	14	103	theme	tissue-engineered	2247:2263	arg1	reconstruction					2270:2283	vascularized tissue-engineered bone reconstruction	2234:2283	vascularized tissue-engineered bone reconstruction	2234:2283	Conclusion The rhCXCL13-HHM/CS scaffold demonstrates excellent potential for BMSC recruitment, osteogenesis, vascularized tissue-engineered bone reconstruction, and drug delivery, providing a theoretical basis for material osteogenesis mechanism study and promising clinical applications for treating large bone defects.
37404852	4	104	theme	recombinant	771:781	arg1	chemokine					801:809	recombinant human C-X-C motif chemokine	771:809	recombinant human C-X-C motif chemokine	771:809	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	2	105	theme	bone	415:418	arg1	marrow					420:425	bone marrow	415:425	bone marrow mesenchymal stem cells (BMSCs)	415:456	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	4	106	theme	C-X-C	789:793	arg1	chemokine					801:809	recombinant human C-X-C motif chemokine	771:809	recombinant human C-X-C motif chemokine	771:809	In this study, The HHM/chitosan (CS) and recombinant human C-X-C motif chemokine ligand 13 (rhCXCL13)-HHM/CS biomimetic scaffolds that optimize bone regeneration and investigated their mechanism of BMSC recruitment and osteogenesis through cell and animal experiments and transcriptomic sequencing.
37404852	2	107	theme	biological	344:353	arg1	activity					355:362	biological activity	344:362	biological activity	344:362	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	6	108	with	co-culture	1281:1290	arg1	BMSCs					1297:1301	BMSCs	1297:1301	BMSCs	1297:1301	Transwell migration experiments and co-culture with BMSCs were conducted to study the recruitment ability and osteogenic differentiation of the scaffolds.
37404852	5	109	theme	X-Ray	1174:1178	arg1	XRD					1193:1195	XRD	1193:1195	XRD	1193:1195	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	5	109	theme	X-Ray	1174:1178	arg1	Diffraction					1180:1190	X-Ray Diffraction	1174:1190	X-Ray Diffraction (XRD)	1174:1196	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	9	110	theme	porous	1692:1697	arg1	network					1717:1723	a porous three-dimensional network	1690:1723	a porous three-dimensional network	1690:1723	Results SEM demonstrated that the rhCXCL13-HHM/CS scaffold comprised hydroxyapatite microspheres in a porous three-dimensional network.
37404852	12	111	theme	rhCXCL13-HHM/CS	1957:1971	arg1	mechanism					1944:1952	the osteogenesis mechanism	1927:1952	the osteogenesis mechanism of rhCXCL13-HHM/CS	1927:1971	Transcriptome sequencing and experimental results showed that the osteogenesis mechanism of rhCXCL13-HHM/CS was through the PI3K-AKT pathway.
37404852	2	112	theme	bone	375:378	arg1	structure					330:338	complex structure	322:338	complex structure	322:338	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	2	112	theme	bone	375:378	arg1	activity					355:362	biological activity	344:362	biological activity	344:362	However, the current methods of preparing composite materials that mimic the complex structure and biological activity of natural bone are challenging for recruitment of bone marrow mesenchymal stem cells (BMSCs), which affects the application of these materials in situ bone regeneration.
37404852	5	113	theme	rhCXCL13	1235:1242	arg1	curve					1226:1230	the cumulative release curve	1203:1230	the cumulative release curve of rhCXCL13	1203:1242	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	5	113	theme	rhCXCL13	1235:1242	arg1	SEM					1168:1170	SEM	1168:1170	SEM	1168:1170	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	5	113	theme	rhCXCL13	1235:1242	arg1	Microscopy					1156:1165	Scanning Electron Microscopy	1138:1165	Scanning Electron Microscopy (SEM)	1138:1171	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	5	113	theme	rhCXCL13	1235:1242	arg1	Diffraction					1180:1190	X-Ray Diffraction	1174:1190	X-Ray Diffraction (XRD)	1174:1196	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
37404852	5	113	theme	rhCXCL13	1235:1242	arg1	XRD					1193:1195	XRD	1193:1195	XRD	1193:1195	Methods Evaluate the physical characteristics of the HHM/CS and rhCXCL13-HHM/CS biomimetic scaffolds through Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD), and the cumulative release curve of rhCXCL13.
36951076	6	0	theme	sugar-water	748:758	arg1	preference					760:769	sugar-water preference	748:769	sugar-water preference	748:769	The behavior of rats was observed by sugar-water preference and forced swimming after modeling.
36951076	12	1	theme	small	2383:2387	arg1	vein					2407:2410	portal vein	2400:2410	portal vein	2400:2410	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	1	theme	small	2383:2387	arg1	artery					2416:2421	artery	2416:2421	artery	2416:2421	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	1	theme	small	2383:2387	arg1	duct					2394:2397	the small bile duct	2379:2397	the small bile duct	2379:2397	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	11	2	from	contents	1889:1896	arg1	serum					1901:1905	serum	1901:1905	serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1901:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	9	3	theme	phosphatidylinositol	1027:1046	arg1	PI3K					1058:1061	PI3K	1058:1061	PI3K	1058:1061	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	9	3	theme	phosphatidylinositol	1027:1046	arg1	3-kinase					1048:1055	phosphatidylinositol 3-kinase	1027:1055	phosphatidylinositol 3-kinase (PI3K)	1027:1062	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	3	4	theme	restraint	461:469	arg1	h					475:475	chronic restraint 2.5 h	453:475	chronic restraint 2.5 h each day for 4 weeks	453:496	The depression model was established by chronic restraint 2.5 h each day for 4 weeks.
36951076	13	5	theme	hepatic	2517:2523	arg1	lobule					2525:2530	the hepatic lobule	2513:2530	the hepatic lobule	2513:2530	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	11	6	theme	immobile	1805:1812	arg1	time					1823:1826	the immobile swimming time	1801:1826	the immobile swimming time	1801:1826	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	7	theme	staining	2830:2837	arg1	intensity					2806:2814	the intensity	2802:2814	the intensity of hepatocyte staining	2802:2837	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	11	8	theme	liver	2018:2022	arg1	tissues					2024:2030	liver tissues	2018:2030	liver tissues	2018:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	10	9	theme	swimming	1357:1364	arg1	P<0.01					1386:1391	P<0.01	1386:1391	P<0.01	1386:1391	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	9	theme	swimming	1357:1364	arg1	prolonged					1375:1383	prolonged	1375:1383	prolonged	1375:1383	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	9	theme	swimming	1357:1364	arg1	time					1366:1369	the immobile swimming time	1344:1369	the immobile swimming time	1344:1369	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	6	10	theme	rats	727:730	arg1	behavior					715:722	The behavior	711:722	The behavior of rats	711:730	The behavior of rats was observed by sugar-water preference and forced swimming after modeling.
36951076	11	11	theme	p-Akt	1957:1961	arg1	proteins					1963:1970	p-Akt proteins	1957:1970	p-Akt proteins	1957:1970	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	9	12	from	levels	1017:1022	arg1	liver					1190:1194	liver	1190:1194	liver	1190:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	14	13	theme	glucose	3042:3048	arg1	metabolism					3050:3059	glucose metabolism	3042:3059	glucose metabolism disorder in chronic restraint induced depressed rats	3042:3112	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	11	14	theme	p-PI3K	1946:1951	arg1	expressions					1931:1941	the expressions	1927:1941	the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1927:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	15	from	granules	2641:2648	arg1	cells					2665:2669	the hepatic cells	2653:2669	the hepatic cells	2653:2669	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	9	16	theme	phosphorylated	1065:1078	arg1	-PI3K					1083:1087	phosphorylated (p)-PI3K	1065:1087	phosphorylated (p)-PI3K (p-PI3K)	1065:1096	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	9	16	theme	phosphorylated	1065:1078	arg1	p-PI3K					1090:1095	p-PI3K	1090:1095	p-PI3K	1090:1095	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	10	17	theme	liver	1532:1536	arg1	tissues					1538:1544	liver tissues	1532:1544	liver tissues	1532:1544	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	1	18	theme	Zusanli	191:197	arg1	ST36					201:204	ST36	201:204	ST36	201:204	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
36951076	1	18	theme	Zusanli	191:197	arg1	"					198:198	"Zusanli"	190:198	"Zusanli" (ST36)	190:205	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
36951076	11	19	dep	decreased	2134:2142	arg1	P<0.01					2145:2150	P<0.01	2145:2150	P<0.01	2145:2150	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	20	theme	ratio	1980:1984	arg1	expressions					1931:1941	the expressions	1927:1941	the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1927:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	10	21	theme	p-Akt/Akt	1519:1527	arg1	ratio					1510:1514	the ratio	1506:1514	the ratio of p-Akt/Akt in liver tissues	1506:1544	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	11	22	from	tissues	2126:2132	arg1	expression					2056:2065	the expression	2052:2065	the expression of p-GSK3β protein	2052:2084	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	22	from	tissues	2126:2132	arg1	ratio					2094:2098	the ratio	2090:2098	the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group	2090:2167	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	4	23	theme	bilateral	576:584	arg1	ST36					586:589	bilateral ST36	576:589	bilateral ST36	576:589	For rats in the EA group, EA stimulation (1 mA, 2 Hz, 30 min) was applied to bilateral ST36 during the modeling period, once a day for 4 weeks.
36951076	11	24	theme	preference	1764:1773	arg1	index					1775:1779	sugar-water preference index	1752:1779	sugar-water preference index	1752:1779	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	25	theme	model	2783:2787	arg1	group					2789:2793	the model group	2779:2793	the model group	2779:2793	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	14	26	theme	PI3K/Akt/GSK3β	3122:3135	arg1	pathway					3147:3153	PI3K/Akt/GSK3β signaling pathway	3122:3153	PI3K/Akt/GSK3β signaling pathway	3122:3153	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	9	27	theme	-PI3K	1083:1087	arg1	levels					1017:1022	The expression levels	1002:1022	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver	1002:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	1	28	theme	depressed	277:285	arg1	rats					287:290	depressed rats	277:290	depressed rats	277:290	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
36951076	11	29	theme	p-PI3K/PI3K	1989:1999	arg1	p-PI3K					1946:1951	p-PI3K	1946:1951	p-PI3K	1946:1951	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	29	theme	p-PI3K/PI3K	1989:1999	arg1	proteins					1963:1970	p-Akt proteins	1957:1970	p-Akt proteins	1957:1970	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	29	theme	p-PI3K/PI3K	1989:1999	arg1	ratio					1980:1984	the ratio	1976:1984	the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1976:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	30	theme	perilobular	2880:2890	arg1	zone					2892:2895	the perilobular zone	2876:2895	the perilobular zone	2876:2895	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	11	31	from	ratio	1980:1984	arg1	tissues					2024:2030	liver tissues	2018:2030	liver tissues	2018:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	7	32	theme	albumin	848:854	arg1	contents					811:818	The contents	807:818	The contents of glucose and glycosylated albumin in serum	807:863	The contents of glucose and glycosylated albumin in serum were determined by biochemical method.
36951076	3	33	dep	day	482:484	arg1	each					477:480	each	477:480	each	477:480	The depression model was established by chronic restraint 2.5 h each day for 4 weeks.
36951076	9	34	theme	3-kinase	1048:1055	arg1	levels					1017:1022	The expression levels	1002:1022	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver	1002:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	11	35	from	p-PI3K	1946:1951	arg1	tissues					2024:2030	liver tissues	2018:2030	liver tissues	2018:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	36	theme	blank	2601:2605	arg1	group					2607:2611	the blank group	2597:2611	the blank group	2597:2611	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	12	37	theme	HE	2170:2171	arg1	staining					2173:2180	HE staining	2170:2180	HE staining	2170:2180	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	11	38	theme	liver	2120:2124	arg1	tissues					2126:2132	liver tissues	2120:2132	liver tissues	2120:2132	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	14	39	theme	chronic	3073:3079	arg1	restraint					3081:3089	chronic restraint	3073:3089	chronic restraint induced depressed rats	3073:3112	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	9	40	theme	Western	1215:1221	arg1	blot					1223:1226	Western blot	1215:1226	Western blot	1215:1226	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	9	41	theme	kinase	1107:1112	arg1	Akt					1117:1119	Akt	1117:1119	Akt	1117:1119	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	9	41	theme	kinase	1107:1112	arg1	B					1114:1114	protein kinase B	1099:1114	protein kinase B (Akt)	1099:1120	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	5	42	theme	rats	666:669	arg1	weight					652:657	The body weight	643:657	The body weight of the rats	643:669	The body weight of the rats was recorded before and after modeling.
36951076	13	43	theme	PAS	2443:2445	arg1	staining					2447:2454	PAS staining	2443:2454	PAS staining	2443:2454	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	1	44	theme	electroacupuncture	163:180	arg1	mechanism					150:158	the mechanism	146:158	the mechanism of electroacupuncture (EA)	146:185	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
36951076	9	45	dep	phosphorylated	1065:1078	arg1	p					1081:1081	p	1081:1081	p	1081:1081	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	11	46	theme	p-GSK3β/GSK3β	2103:2115	arg1	expression					2056:2065	the expression	2052:2065	the expression of p-GSK3β protein	2052:2084	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	46	theme	p-GSK3β/GSK3β	2103:2115	arg1	ratio					2094:2098	the ratio	2090:2098	the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group	2090:2167	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	47	theme	zone	2892:2895	arg1	intensity					2863:2871	the staining intensity	2850:2871	the staining intensity of the perilobular zone	2850:2895	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	13	47	theme	zone	2892:2895	arg1	weaker					2901:2906	weaker	2901:2906	weaker	2901:2906	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	12	48	theme	bile	2389:2392	arg1	vein					2407:2410	portal vein	2400:2410	portal vein	2400:2410	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	48	theme	bile	2389:2392	arg1	artery					2416:2421	artery	2416:2421	artery	2416:2421	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	48	theme	bile	2389:2392	arg1	duct					2394:2397	the small bile duct	2379:2397	the small bile duct	2379:2397	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	11	49	theme	EA	2160:2161	arg1	group					2163:2167	the EA group	2156:2167	the EA group	2156:2167	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	9	50	theme	p-Akt	1123:1127	arg1	levels					1017:1022	The expression levels	1002:1022	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver	1002:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	10	51	theme	control	1255:1261	arg1	group					1263:1267	the control group	1251:1267	the control group	1251:1267	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	11	52	theme	p-GSK3β	2070:2076	arg1	protein					2078:2084	p-GSK3β protein	2070:2084	p-GSK3β protein	2070:2084	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	53	theme	staining	2485:2492	arg1	intensity					2472:2480	the intensity	2468:2480	the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule	2468:2569	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	10	54	theme	glycosylated	1411:1422	arg1	contents					1432:1439	glycosylated albumin contents	1411:1439	glycosylated albumin contents	1411:1439	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	9	55	theme	glycogen	1130:1137	arg1	synthase					1139:1146	glycogen synthase kinase-3β	1130:1156	glycogen synthase kinase-3β (GSK3β)	1130:1164	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	9	55	theme	glycogen	1130:1137	arg1	GSK3β					1159:1163	GSK3β	1159:1163	GSK3β	1159:1163	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	8	56	theme	histopathological	908:924	arg1	morphology					926:935	The histopathological morphology	904:935	The histopathological morphology	904:935	The histopathological morphology and liver glycogen content were observed by HE and PAS staining.
36951076	12	57	theme	portal	2430:2435	arg1	area					2437:2440	the portal area	2426:2440	the portal area	2426:2440	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	58	theme	hepatic	2215:2221	arg1	lobule					2223:2228	the hepatic lobule	2211:2228	the hepatic lobule	2211:2228	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	0	59	theme	glucose	30:36	arg1	disorder					49:56	glucose metabolism disorder	30:56	glucose metabolism disorder	30:56	[Electroacupuncture regulates glucose metabolism disorder through PI3K/Akt/GSK3β signaling pathway in rats with depression].
36951076	11	60	from	tissues	2024:2030	arg1	p-PI3K					1946:1951	p-PI3K	1946:1951	p-PI3K	1946:1951	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	60	from	tissues	2024:2030	arg1	proteins					1963:1970	p-Akt proteins	1957:1970	p-Akt proteins	1957:1970	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	60	from	tissues	2024:2030	arg1	ratio					1980:1984	the ratio	1976:1984	the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1976:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	3	61	dep	h	475:475	arg1	day					482:484	day	482:484	day	482:484	The depression model was established by chronic restraint 2.5 h each day for 4 weeks.
36951076	12	62	theme	portal	2400:2405	arg1	vein					2407:2410	portal vein	2400:2410	portal vein	2400:2410	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	62	theme	portal	2400:2405	arg1	duct					2394:2397	the small bile duct	2379:2397	the small bile duct	2379:2397	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	10	63	theme	weight	1274:1279	arg1	increment					1281:1289	the weight increment	1270:1289	the weight increment	1270:1289	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	12	64	located	observed	2367:2374	arg1	vein					2407:2410	portal vein	2400:2410	portal vein	2400:2410	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	64	located	observed	2367:2374	arg2	abnormalities					2348:2360	no abnormalities	2345:2360	no abnormalities	2345:2360	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	64	located	observed	2367:2374	arg1	area					2437:2440	the portal area	2426:2440	the portal area	2426:2440	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	64	located	observed	2367:2374	arg1	artery					2416:2421	artery	2416:2421	artery	2416:2421	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	64	located	observed	2367:2374	arg1	duct					2394:2397	the small bile duct	2379:2397	the small bile duct	2379:2397	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	8	65	theme	glycogen	947:954	arg1	content					956:962	liver glycogen content	941:962	liver glycogen content	941:962	The histopathological morphology and liver glycogen content were observed by HE and PAS staining.
36951076	13	66	theme	hepatic	2556:2562	arg1	lobule					2564:2569	the hepatic lobule	2552:2569	the hepatic lobule	2552:2569	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	11	67	theme	glycosylated	1868:1879	arg1	contents					1889:1896	glycosylated albumin contents	1868:1896	glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1868:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	14	68	theme	CONCLUSION	3002:3011	arg1	intervention					3016:3027	CONCLUSION EA intervention	3002:3027	CONCLUSION EA intervention	3002:3027	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	10	69	theme	p-GSK3β	1585:1591	arg1	protein					1593:1599	p-GSK3β protein	1585:1599	p-GSK3β protein	1585:1599	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	2	70	theme	rats	323:326	arg1	total					303:307	A total	301:307	METHODS A total of 30 male SD rats	293:326	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	5	71	theme	body	647:650	arg1	weight					652:657	The body weight	643:657	The body weight of the rats	643:669	The body weight of the rats was recorded before and after modeling.
36951076	11	72	from	ratio	2094:2098	arg1	tissues					2126:2132	liver tissues	2120:2132	liver tissues	2120:2132	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	10	73	from	contents	1432:1439	arg1	serum					1444:1448	serum	1444:1448	serum	1444:1448	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	74	dep	increased	1649:1657	arg1	P<0.001					1667:1673	P<0.001	1667:1673	P<0.001	1667:1673	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	74	dep	increased	1649:1657	arg1	P<0.01					1660:1665	P<0.01	1660:1665	P<0.01	1660:1665	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	75	theme	preference	1307:1316	arg1	index					1318:1322	sugar-water preference index	1295:1322	sugar-water preference index	1295:1322	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	76	theme	p-GSK3β/GSK3β	1618:1630	arg1	protein					1593:1599	p-GSK3β protein	1585:1599	p-GSK3β protein	1585:1599	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	76	theme	p-GSK3β/GSK3β	1618:1630	arg1	ratio					1609:1613	the ratio	1605:1613	the ratio of p-GSK3β/GSK3β	1605:1630	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	2	77	theme	male	315:318	arg1	rats					323:326	30 male SD rats	312:326	30 male SD rats	312:326	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	9	78	theme	proteins	1178:1185	arg1	levels					1017:1022	The expression levels	1002:1022	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver	1002:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	12	79	theme	obvious	2245:2251	arg1	infiltration					2271:2282	no obvious inflammatory cell infiltration	2242:2282	no obvious inflammatory cell infiltration	2242:2282	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	10	80	theme	ratio	1609:1613	arg1	expression					1571:1580	the expression	1567:1580	the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues	1567:1647	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	9	81	theme	expression	1006:1015	arg1	levels					1017:1022	The expression levels	1002:1022	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver	1002:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	10	82	theme	protein	1494:1500	arg1	expression					1474:1483	the expression	1470:1483	the expression of p-Akt protein	1470:1500	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	1	83	theme	glucose	220:226	arg1	metabolism					228:237	glucose metabolism	220:237	glucose metabolism disorder	220:246	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
36951076	13	84	from	center	2503:2508	arg1	intensity					2472:2480	the intensity	2468:2480	the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule	2468:2569	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	14	85	from	disorder	3061:3068	arg1	restraint					3081:3089	chronic restraint	3073:3089	chronic restraint induced depressed rats	3073:3112	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	11	86	dep	increased	1781:1789	arg1	P<0.05					1792:1797	P<0.05	1792:1797	P<0.05	1792:1797	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	3	87	theme	chronic	453:459	arg1	restraint					461:469	chronic restraint	453:469	chronic restraint 2.5 h each day for 4 weeks	453:496	The depression model was established by chronic restraint 2.5 h each day for 4 weeks.
36951076	10	88	theme	liver	1635:1639	arg1	tissues					1641:1647	liver tissues	1635:1647	liver tissues	1635:1647	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	0	89	with	rats	102:105	arg1	depression					112:121	depression]	112:122	depression]	112:122	[Electroacupuncture regulates glucose metabolism disorder through PI3K/Akt/GSK3β signaling pathway in rats with depression].
36951076	12	90	theme	cell	2266:2269	arg1	infiltration					2271:2282	no obvious inflammatory cell infiltration	2242:2282	no obvious inflammatory cell infiltration	2242:2282	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	1	91	theme	chronic	251:257	arg1	restraint					259:267	chronic restraint	251:267	chronic restraint	251:267	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
36951076	11	92	theme	swimming	1814:1821	arg1	time					1823:1826	the immobile swimming time	1801:1826	the immobile swimming time	1801:1826	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	4	93	theme	EA	515:516	arg1	group					518:522	the EA group	511:522	the EA group	511:522	For rats in the EA group, EA stimulation (1 mA, 2 Hz, 30 min) was applied to bilateral ST36 during the modeling period, once a day for 4 weeks.
36951076	10	94	theme	immobile	1348:1355	arg1	P<0.01					1386:1391	P<0.01	1386:1391	P<0.01	1386:1391	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	94	theme	immobile	1348:1355	arg1	prolonged					1375:1383	prolonged	1375:1383	prolonged	1375:1383	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	94	theme	immobile	1348:1355	arg1	time					1366:1369	the immobile swimming time	1344:1369	the immobile swimming time	1344:1369	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	95	dep	prolonged	1375:1383	arg1	P<0.01					1386:1391	P<0.01	1386:1391	P<0.01	1386:1391	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	95	dep	prolonged	1375:1383	arg1	prolonged					1375:1383	prolonged	1375:1383	prolonged	1375:1383	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	95	dep	prolonged	1375:1383	arg1	time					1366:1369	the immobile swimming time	1344:1369	the immobile swimming time	1344:1369	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	11	96	theme	model	1714:1718	arg1	group					1720:1724	the model group	1710:1724	the model group	1710:1724	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	0	97	theme	PI3K/Akt/GSK3β	66:79	arg1	pathway					91:97	PI3K/Akt/GSK3β signaling pathway	66:97	PI3K/Akt/GSK3β signaling pathway in rats with depression]	66:122	[Electroacupuncture regulates glucose metabolism disorder through PI3K/Akt/GSK3β signaling pathway in rats with depression].
36951076	10	98	from	glucose	1399:1405	arg1	serum					1444:1448	serum	1444:1448	serum	1444:1448	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	0	99	from	pathway	91:97	arg1	rats					102:105	rats	102:105	rats with depression]	102:122	[Electroacupuncture regulates glucose metabolism disorder through PI3K/Akt/GSK3β signaling pathway in rats with depression].
36951076	9	100	theme	protein	1099:1105	arg1	Akt					1117:1119	Akt	1117:1119	Akt	1117:1119	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	9	100	theme	protein	1099:1105	arg1	B					1114:1114	protein kinase B	1099:1114	protein kinase B (Akt)	1099:1120	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	14	101	theme	depressed	3099:3107	arg1	rats					3109:3112	depressed rats	3099:3112	depressed rats	3099:3112	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	13	102	theme	glycogen-rich	2627:2639	arg1	granules					2641:2648	the glycogen-rich granules	2623:2648	the glycogen-rich granules in the hepatic cells	2623:2669	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	13	103	theme	lobule	2525:2530	arg1	center					2503:2508	the center	2499:2508	the center of the hepatic lobule to the periphery of the hepatic lobule	2499:2569	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	10	104	theme	model	1683:1687	arg1	group					1689:1693	the model group	1679:1693	the model group	1679:1693	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	11	105	dep	increased	2032:2040	arg1	P<0.05					2043:2048	P<0.05	2043:2048	P<0.05	2043:2048	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	106	theme	weight	1731:1736	arg1	increment					1738:1746	the weight increment	1727:1746	the weight increment	1727:1746	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	107	theme	sugar-water	1752:1762	arg1	index					1775:1779	sugar-water preference index	1752:1779	sugar-water preference index	1752:1779	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	108	from	p-GSK3β/GSK3β	2103:2115	arg1	tissues					2126:2132	liver tissues	2120:2132	liver tissues	2120:2132	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	13	109	theme	hepatocyte	2819:2828	arg1	staining					2830:2837	hepatocyte staining	2819:2837	hepatocyte staining	2819:2837	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	11	110	theme	proteins	1963:1970	arg1	expressions					1931:1941	the expressions	1927:1941	the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1927:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	10	111	dep	decreased	1546:1554	arg1	P<0.001					1557:1563	P<0.001	1557:1563	P<0.001	1557:1563	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	11	112	dep	shortened	1832:1840	arg1	P<0.05					1843:1848	P<0.05	1843:1848	P<0.05	1843:1848	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	113	from	expression	2056:2065	arg1	tissues					2126:2132	liver tissues	2120:2132	liver tissues	2120:2132	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	114	from	proteins	1963:1970	arg1	tissues					2024:2030	liver tissues	2018:2030	liver tissues	2018:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	115	from	p-PI3K/PI3K	1989:1999	arg1	tissues					2024:2030	liver tissues	2018:2030	liver tissues	2018:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	7	116	theme	biochemical	884:894	arg1	method					896:901	biochemical method	884:901	biochemical method	884:901	The contents of glucose and glycosylated albumin in serum were determined by biochemical method.
36951076	6	117	theme	forced	775:780	arg1	swimming					782:789	forced swimming	775:789	forced swimming	775:789	The behavior of rats was observed by sugar-water preference and forced swimming after modeling.
36951076	11	118	theme	protein	2078:2084	arg1	expression					2056:2065	the expression	2052:2065	the expression of p-GSK3β protein	2052:2084	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	118	theme	protein	2078:2084	arg1	ratio					2094:2098	the ratio	2090:2098	the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group	2090:2167	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	2	119	dep	METHODS	293:299	arg1	total					303:307	A total	301:307	METHODS A total of 30 male SD rats	293:326	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	4	120	dep	stimulation	528:538	arg1	mA					543:544	1 mA	541:544	1 mA	541:544	For rats in the EA group, EA stimulation (1 mA, 2 Hz, 30 min) was applied to bilateral ST36 during the modeling period, once a day for 4 weeks.
36951076	4	120	dep	stimulation	528:538	arg1	Hz					549:550	2 Hz	547:550	2 Hz	547:550	For rats in the EA group, EA stimulation (1 mA, 2 Hz, 30 min) was applied to bilateral ST36 during the modeling period, once a day for 4 weeks.
36951076	12	121	theme	inflammatory	2253:2264	arg1	infiltration					2271:2282	no obvious inflammatory cell infiltration	2242:2282	no obvious inflammatory cell infiltration	2242:2282	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	10	122	gly	glycosylated	1411:1422	arg1	contents					1432:1439	glycosylated albumin contents	1411:1439	glycosylated albumin contents	1411:1439	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	7	123	theme	glycosylated	835:846	arg1	albumin					848:854	glycosylated albumin	835:854	glycosylated albumin	835:854	The contents of glucose and glycosylated albumin in serum were determined by biochemical method.
36951076	10	124	from	ratio	1510:1514	arg1	tissues					1538:1544	liver tissues	1532:1544	liver tissues	1532:1544	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	2	125	from	rats	393:396	arg1	group					406:410	each group	401:410	each group	401:410	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	13	126	theme	EA	2992:2993	arg1	group					2995:2999	the EA group	2988:2999	the EA group	2988:2999	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	1	127	dep	OBJECTIVE	125:133	arg1	explore					138:144	explore	138:144	To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint	135:267	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
36951076	10	128	dep	decreased	1324:1332	arg1	P<0.01					1335:1340	P<0.01	1335:1340	P<0.01	1335:1340	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	9	129	theme	B	1114:1114	arg1	levels					1017:1022	The expression levels	1002:1022	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver	1002:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	13	130	theme	blank	2925:2929	arg1	group					2931:2935	the blank group	2921:2935	the blank group	2921:2935	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	7	131	theme	glucose	823:829	arg1	contents					811:818	The contents	807:818	The contents of glucose and glycosylated albumin in serum	807:863	The contents of glucose and glycosylated albumin in serum were determined by biochemical method.
36951076	7	132	from	contents	811:818	arg1	serum					859:863	serum	859:863	serum	859:863	The contents of glucose and glycosylated albumin in serum were determined by biochemical method.
36951076	14	133	theme	metabolism	3050:3059	arg1	disorder					3061:3068	glucose metabolism disorder	3042:3068	glucose metabolism disorder in chronic restraint induced depressed rats	3042:3112	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	11	134	from	p-Akt/Akt	2005:2013	arg1	tissues					2024:2030	liver tissues	2018:2030	liver tissues	2018:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	14	135	theme	signaling	3137:3145	arg1	pathway					3147:3153	PI3K/Akt/GSK3β signaling pathway	3122:3153	PI3K/Akt/GSK3β signaling pathway	3122:3153	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	2	136	theme	model	364:368	arg1	groups					377:382	control, model and EA groups	355:382	groups	377:382	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	4	137	from	rats	503:506	arg1	group					518:522	the EA group	511:522	the EA group	511:522	For rats in the EA group, EA stimulation (1 mA, 2 Hz, 30 min) was applied to bilateral ST36 during the modeling period, once a day for 4 weeks.
36951076	12	138	located	observed	2300:2307	arg1	lobule					2316:2321	lobule	2316:2321	lobule	2316:2321	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	138	located	observed	2300:2307	arg2	fibrosis					2287:2294	fibrosis	2287:2294	fibrosis	2287:2294	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	138	located	observed	2300:2307	arg2	infiltration					2271:2282	no obvious inflammatory cell infiltration	2242:2282	no obvious inflammatory cell infiltration	2242:2282	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	138	located	observed	2300:2307	arg1	interstitium					2327:2338	interstitium	2327:2338	interstitium	2327:2338	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	7	139	gly	glycosylated	835:846	arg1	albumin					848:854	glycosylated albumin	835:854	glycosylated albumin	835:854	The contents of glucose and glycosylated albumin in serum were determined by biochemical method.
36951076	12	140	theme	lobule	2223:2228	arg1	intact					2234:2239	intact	2234:2239	intact	2234:2239	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	12	140	theme	lobule	2223:2228	arg1	structure					2198:2206	the structure	2194:2206	the structure of the hepatic lobule	2194:2228	HE staining showed that the structure of the hepatic lobule was intact, no obvious inflammatory cell infiltration or fibrosis was observed in the lobule and interstitium, and no abnormalities were observed in the small bile duct, portal vein and artery in the portal area.
36951076	4	141	theme	modeling	602:609	arg1	period					611:616	the modeling period	598:616	the modeling period	598:616	For rats in the EA group, EA stimulation (1 mA, 2 Hz, 30 min) was applied to bilateral ST36 during the modeling period, once a day for 4 weeks.
36951076	2	142	theme	control	355:361	arg1	groups					377:382	control, model and EA groups	355:382	groups	377:382	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	0	143	theme	metabolism	38:47	arg1	disorder					49:56	glucose metabolism disorder	30:56	glucose metabolism disorder	30:56	[Electroacupuncture regulates glucose metabolism disorder through PI3K/Akt/GSK3β signaling pathway in rats with depression].
36951076	9	144	theme	synthase	1139:1146	arg1	levels					1017:1022	The expression levels	1002:1022	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver	1002:1194	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	8	145	theme	liver	941:945	arg1	content					956:962	liver glycogen content	941:962	liver glycogen content	941:962	The histopathological morphology and liver glycogen content were observed by HE and PAS staining.
36951076	11	146	dep	decreased	1907:1915	arg1	P<0.05					1918:1923	P<0.05	1918:1923	P<0.05	1918:1923	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	3	147	theme	depression	417:426	arg1	model					428:432	The depression model	413:432	The depression model	413:432	The depression model was established by chronic restraint 2.5 h each day for 4 weeks.
36951076	10	148	from	expression	1571:1580	arg1	tissues					1641:1647	liver tissues	1635:1647	liver tissues	1635:1647	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	14	149	theme	EA	3013:3014	arg1	intervention					3016:3027	CONCLUSION EA intervention	3002:3027	CONCLUSION EA intervention	3002:3027	CONCLUSION EA intervention can regulate glucose metabolism disorder in chronic restraint induced depressed rats through PI3K/Akt/GSK3β signaling pathway.
36951076	13	150	theme	glycogen	2946:2953	arg1	particles					2955:2963	the glycogen particles	2942:2963	the glycogen particles	2942:2963	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	10	151	theme	protein	1593:1599	arg1	expression					1571:1580	the expression	1567:1580	the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues	1567:1647	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	2	152	theme	EA	374:375	arg1	groups					377:382	control, model and EA groups	355:382	groups	377:382	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	0	153	theme	signaling	81:89	arg1	pathway					91:97	PI3K/Akt/GSK3β signaling pathway	66:97	PI3K/Akt/GSK3β signaling pathway in rats with depression]	66:122	[Electroacupuncture regulates glucose metabolism disorder through PI3K/Akt/GSK3β signaling pathway in rats with depression].
36951076	13	154	theme	staining	2854:2861	arg1	intensity					2863:2871	the staining intensity	2850:2871	the staining intensity of the perilobular zone	2850:2895	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	13	154	theme	staining	2854:2861	arg1	weaker					2901:2906	weaker	2901:2906	weaker	2901:2906	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	13	155	theme	lobule	2564:2569	arg1	periphery					2539:2547	the periphery	2535:2547	the periphery of the hepatic lobule	2535:2569	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	11	156	theme	albumin	1881:1887	arg1	contents					1889:1896	glycosylated albumin contents	1868:1896	glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1868:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	10	157	dep	increased	1450:1458	arg1	P<0.05					1461:1466	P<0.05	1461:1466	P<0.05	1461:1466	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	2	158	theme	SD	320:321	arg1	rats					323:326	30 male SD rats	312:326	30 male SD rats	312:326	METHODS A total of 30 male SD rats were randomly divided into control, model and EA groups, with 10 rats in each group.
36951076	9	159	theme	p-GSK3β	1170:1176	arg1	proteins					1178:1185	p-GSK3β proteins	1170:1185	p-GSK3β proteins	1170:1185	The expression levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated (p)-PI3K (p-PI3K), protein kinase B (Akt), p-Akt, glycogen synthase kinase-3β (GSK3β) and p-GSK3β proteins in liver were determined by Western blot.
36951076	11	160	gly	glycosylated	1868:1879	arg1	contents					1889:1896	glycosylated albumin contents	1868:1896	glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1868:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	161	theme	p-Akt/Akt	2005:2013	arg1	p-PI3K					1946:1951	p-PI3K	1946:1951	p-PI3K	1946:1951	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	161	theme	p-Akt/Akt	2005:2013	arg1	proteins					1963:1970	p-Akt proteins	1957:1970	p-Akt proteins	1957:1970	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	11	161	theme	p-Akt/Akt	2005:2013	arg1	ratio					1980:1984	the ratio	1976:1984	the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues	1976:2030	Compared with the model group, the weight increment and sugar-water preference index increased (P<0.05), the immobile swimming time was shortened (P<0.05), the glucose and glycosylated albumin contents in serum decreased (P<0.05), the expressions of p-PI3K and p-Akt proteins and the ratio of p-PI3K/PI3K and p-Akt/Akt in liver tissues increased (P<0.05), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues decreased (P<0.01) in the EA group.
36951076	4	162	theme	EA	525:526	arg1	stimulation					528:538	EA stimulation	525:538	EA stimulation (1 mA, 2 Hz, 30 min)	525:559	For rats in the EA group, EA stimulation (1 mA, 2 Hz, 30 min) was applied to bilateral ST36 during the modeling period, once a day for 4 weeks.
36951076	10	163	theme	sugar-water	1295:1305	arg1	index					1318:1322	sugar-water preference index	1295:1322	sugar-water preference index	1295:1322	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	8	164	theme	PAS	988:990	arg1	staining					992:999	PAS staining	988:999	PAS staining	988:999	The histopathological morphology and liver glycogen content were observed by HE and PAS staining.
36951076	13	165	theme	hepatic	2657:2663	arg1	cells					2665:2669	the hepatic cells	2653:2669	the hepatic cells	2653:2669	PAS staining showed that the intensity of staining from the center of the hepatic lobule to the periphery of the hepatic lobule was gradually enhanced in the blank group, that is, the glycogen-rich granules in the hepatic cells were gradually increased; most of the hepatocytes were light colored and glycogen was lost significantly in the model group; while the intensity of hepatocyte staining increased, the staining intensity of the perilobular zone was weaker than that in the blank group, and the glycogen particles partially recovered in the EA group.
36951076	10	166	theme	albumin	1424:1430	arg1	contents					1432:1439	glycosylated albumin contents	1411:1439	glycosylated albumin contents	1411:1439	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	10	167	theme	p-Akt	1488:1492	arg1	protein					1494:1500	p-Akt protein	1488:1500	p-Akt protein	1488:1500	RESULTS Compared with the control group, the weight increment and sugar-water preference index decreased (P<0.01), the immobile swimming time was prolonged (P<0.01), the glucose and glycosylated albumin contents in serum increased (P<0.05), the expression of p-Akt protein and the ratio of p-Akt/Akt in liver tissues decreased (P<0.001), the expression of p-GSK3β protein and the ratio of p-GSK3β/GSK3β in liver tissues increased (P<0.01,P<0.001) in the model group.
36951076	1	168	theme	metabolism	228:237	arg1	disorder					239:246	glucose metabolism disorder	220:246	glucose metabolism disorder	220:246	OBJECTIVE To explore the mechanism of electroacupuncture (EA) at "Zusanli" (ST36) on improving glucose metabolism disorder in chronic restraint induced depressed rats.
35504473	10	0	from	response	1606:1613	arg1	sensing					1623:1629	H2O2 sensing	1618:1629	H2O2 sensing	1618:1629	However, Cs/Se NCs did not show good response in H2O2 sensing than the Cs/Ag NCs, implying the biocompatibility of Cs/Ag NCs.
35504473	7	1	theme	NPs	1291:1293	arg1	activity					1264:1271	the effective inhibitory activity	1239:1271	the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria	1239:1349	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	6	2	theme	sensing	935:941	arg1	properties					943:952	hydrogen peroxide (H2O2) sensing properties	910:952	hydrogen peroxide (H2O2) sensing properties	910:952	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	3	3	theme	Se	570:571	arg1	NPs					573:575	Se NPs	570:575	Se NPs	570:575	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	3	theme	Se	570:571	arg1	nanoparticles					555:567	selenium nanoparticles	546:567	selenium nanoparticles (Se NPs)	546:576	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	10	4	theme	H2O2	1618:1621	arg1	sensing					1623:1629	H2O2 sensing	1618:1629	H2O2 sensing	1618:1629	However, Cs/Se NCs did not show good response in H2O2 sensing than the Cs/Ag NCs, implying the biocompatibility of Cs/Ag NCs.
35504473	9	5	theme	Cs/Ag	1494:1498	arg1	NCs					1500:1502	Cs/Ag NCs	1494:1502	Cs/Ag NCs	1494:1502	Intriguingly, the embryo treated with Cs/Se NCs and Cs/Ag NCs significantly reduced the toxicity in the presence of Cs matrix.
35504473	9	6	theme	Cs/Se	1480:1484	arg1	NCs					1486:1488	Cs/Se NCs	1480:1488	Cs/Se NCs	1480:1488	Intriguingly, the embryo treated with Cs/Se NCs and Cs/Ag NCs significantly reduced the toxicity in the presence of Cs matrix.
35504473	0	7	theme	nanocomposites	140:153	arg1	assessment					23:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	0	7	theme	nanocomposites	140:153	arg1	potential					49:57	biosensing potential	38:57	biosensing potential	38:57	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	3	8	theme	chitosan/selenium	579:595	arg1	nanocomposites					597:610	chitosan/selenium nanocomposites	579:610	chitosan/selenium nanocomposites (Cs/Se NCs)	579:622	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	8	theme	chitosan/selenium	579:595	arg1	NCs					619:621	Cs/Se NCs	613:621	Cs/Se NCs	613:621	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	1	9	used	used	248:251	arg2	H2O2					232:235	H2O2	232:235	H2O2	232:235	Hydrogen peroxide (H2O2) is widely used in various industries and biological fields.
35504473	1	9	used	used	248:251	arg2	peroxide					222:229	Hydrogen peroxide	213:229	Hydrogen peroxide (H2O2)	213:236	Hydrogen peroxide (H2O2) is widely used in various industries and biological fields.
35504473	10	10	theme	Cs/Ag	1640:1644	arg1	NCs					1646:1648	the Cs/Ag NCs	1636:1648	the Cs/Ag NCs	1636:1648	However, Cs/Se NCs did not show good response in H2O2 sensing than the Cs/Ag NCs, implying the biocompatibility of Cs/Ag NCs.
35504473	11	11	theme	environmental	1825:1837	arg1	biosensors					1849:1858	environmental pollutant biosensors	1825:1858	environmental pollutant biosensors	1825:1858	Overall, the obtained results clearly suggest that Cs/Ag NCs could be suitable for dual applications such as for the detection of environmental pollutant biosensors and for biomedical research.
35504473	0	12	theme	silver	156:161	arg1	nanoparticles					163:175	silver nanoparticles	156:175	silver nanoparticles	156:175	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	7	13	theme	antibacterial	1184:1196	arg1	results					1210:1216	antibacterial interaction results	1184:1216	antibacterial interaction results	1184:1216	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	6	14	theme	cyclic	996:1001	arg1	spectroscopy					1047:1058	electrochemical impedance spectroscopy	1021:1058	electrochemical impedance spectroscopy (EIS)	1021:1064	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	14	theme	cyclic	996:1001	arg1	CV					1016:1017	CV	1016:1017	CV	1016:1017	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	14	theme	cyclic	996:1001	arg1	voltammetry					1003:1013	cyclic voltammetry	996:1013	cyclic voltammetry (CV)	996:1018	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	14	theme	cyclic	996:1001	arg1	methods					1093:1099	chronoamperometry (CA) methods	1070:1099	chronoamperometry (CA) methods	1070:1099	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	15	theme	sensing	1139:1145	arg1	properties					1147:1156	pronounced sensing properties	1128:1156	pronounced sensing properties	1128:1156	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	8	16	theme	highest	1356:1362	arg1	embryotoxicity					1364:1377	The highest embryotoxicity	1352:1377	The highest embryotoxicity	1352:1377	The highest embryotoxicity was recorded at 0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs.
35504473	0	17	theme	nanocomposites	197:210	arg1	assessment					23:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	0	17	theme	nanocomposites	197:210	arg1	potential					49:57	biosensing potential	38:57	biosensing potential	38:57	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	3	18	theme	Ag	647:648	arg1	nanoparticles					632:644	silver nanoparticles	625:644	silver nanoparticles (Ag NPs)	625:653	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	18	theme	Ag	647:648	arg1	NPs					650:652	Ag NPs	647:652	Ag NPs	647:652	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	11	19	theme	Cs/Ag	1746:1750	arg1	NCs					1752:1754	Cs/Ag NCs	1746:1754	Cs/Ag NCs	1746:1754	Overall, the obtained results clearly suggest that Cs/Ag NCs could be suitable for dual applications such as for the detection of environmental pollutant biosensors and for biomedical research.
35504473	5	20	theme	physico-chemical	773:788	arg1	properties					790:799	The physico-chemical properties	769:799	The physico-chemical properties of as-synthesized nanomaterials	769:831	The physico-chemical properties of as-synthesized nanomaterials were confirmed by various spectroscopic and microscopic techniques.
35504473	0	21	theme	toxicological	9:21	arg1	assessment					23:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	9	22	theme	Cs	1558:1559	arg1	matrix					1561:1566	Cs matrix	1558:1566	Cs matrix	1558:1566	Intriguingly, the embryo treated with Cs/Se NCs and Cs/Ag NCs significantly reduced the toxicity in the presence of Cs matrix.
35504473	11	23	theme	dual	1778:1781	arg1	applications					1783:1794	dual applications	1778:1794	dual applications such as for the detection of environmental pollutant biosensors and for biomedical research	1778:1886	Overall, the obtained results clearly suggest that Cs/Ag NCs could be suitable for dual applications such as for the detection of environmental pollutant biosensors and for biomedical research.
35504473	0	24	theme	nanoparticles	83:95	arg1	assessment					23:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	0	24	theme	nanoparticles	83:95	arg1	potential					49:57	biosensing potential	38:57	biosensing potential	38:57	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	0	25	theme	selenium	98:105	arg1	nanoparticles					107:119	selenium nanoparticles	98:119	selenium nanoparticles	98:119	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	6	26	theme	electrochemical	1021:1035	arg1	spectroscopy					1047:1058	electrochemical impedance spectroscopy	1021:1058	electrochemical impedance spectroscopy (EIS)	1021:1064	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	26	theme	electrochemical	1021:1035	arg1	voltammetry					1003:1013	cyclic voltammetry	996:1013	cyclic voltammetry (CV)	996:1018	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	26	theme	electrochemical	1021:1035	arg1	EIS					1061:1063	EIS	1061:1063	EIS	1061:1063	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	3	27	theme	chitosan	513:520	arg1	nanoparticles					522:534	bioinspired chitosan nanoparticles	501:534	bioinspired chitosan nanoparticles (Cs NPs)	501:543	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	27	theme	chitosan	513:520	arg1	NPs					540:542	Cs NPs	537:542	Cs NPs	537:542	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	8	28	theme	NPs	1412:1414	arg1	0.19 μg/ml					1395:1404	0.19 μg/ml	1395:1404	0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs	1395:1439	The highest embryotoxicity was recorded at 0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs.
35504473	0	29	theme	biosensing	38:47	arg1	potential					49:57	biosensing potential	38:57	biosensing potential	38:57	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	3	30	theme	present	420:426	arg1	study					428:432	The present study	416:432	The present study	416:432	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	0	31	theme	chitosan	74:81	arg1	nanoparticles					83:95	bioinspired chitosan nanoparticles	62:95	bioinspired chitosan nanoparticles	62:95	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	8	32	theme	1.56 μg/ml	1420:1429	arg1	0.19 μg/ml					1395:1404	0.19 μg/ml	1395:1404	0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs	1395:1439	The highest embryotoxicity was recorded at 0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs.
35504473	0	33	theme	In	0:1	arg1	assessment					23:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	11	34	theme	obtained	1708:1715	arg1	results					1717:1723	the obtained results	1704:1723	the obtained results	1704:1723	Overall, the obtained results clearly suggest that Cs/Ag NCs could be suitable for dual applications such as for the detection of environmental pollutant biosensors and for biomedical research.
35504473	8	35	theme	Se	1434:1435	arg1	NPs					1437:1439	Se NPs	1434:1439	Se NPs	1434:1439	The highest embryotoxicity was recorded at 0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs.
35504473	3	36	theme	bioinspired	501:511	arg1	nanoparticles					522:534	bioinspired chitosan nanoparticles	501:534	bioinspired chitosan nanoparticles (Cs NPs)	501:543	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	36	theme	bioinspired	501:511	arg1	NPs					540:542	Cs NPs	537:542	Cs NPs	537:542	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	7	37	theme	interaction	1198:1208	arg1	results					1210:1216	antibacterial interaction results	1184:1216	antibacterial interaction results	1184:1216	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	0	38	theme	bioinspired	62:72	arg1	nanoparticles					83:95	bioinspired chitosan nanoparticles	62:95	bioinspired chitosan nanoparticles	62:95	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	3	39	theme	chitosan/silver	659:673	arg1	nanocomposites					675:688	chitosan/silver nanocomposites	659:688	chitosan/silver nanocomposites	659:688	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	10	40	theme	Cs/Ag	1684:1688	arg1	NCs					1690:1692	Cs/Ag NCs	1684:1692	Cs/Ag NCs	1684:1692	However, Cs/Se NCs did not show good response in H2O2 sensing than the Cs/Ag NCs, implying the biocompatibility of Cs/Ag NCs.
35504473	5	41	theme	nanomaterials	819:831	arg1	properties					790:799	The physico-chemical properties	769:799	The physico-chemical properties of as-synthesized nanomaterials	769:831	The physico-chemical properties of as-synthesized nanomaterials were confirmed by various spectroscopic and microscopic techniques.
35504473	6	42	theme	hydrogen	910:917	arg1	H2O2					929:932	H2O2	929:932	H2O2	929:932	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	42	theme	hydrogen	910:917	arg1	peroxide					919:926	hydrogen peroxide	910:926	hydrogen peroxide (H2O2) sensing properties	910:952	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	7	43	theme	Ag	1288:1289	arg1	NPs					1291:1293	as-prepared Ag NPs	1276:1293	as-prepared Ag NPs	1276:1293	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	3	44	theme	Cs	537:538	arg1	nanoparticles					522:534	bioinspired chitosan nanoparticles	501:534	bioinspired chitosan nanoparticles (Cs NPs)	501:543	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	44	theme	Cs	537:538	arg1	NPs					540:542	Cs NPs	537:542	Cs NPs	537:542	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	7	45	theme	pathogenic	1331:1340	arg1	bacteria					1342:1349	Gram negative pathogenic bacteria	1317:1349	Gram negative pathogenic bacteria	1317:1349	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	3	46	theme	nanoparticles	632:644	arg1	antimicrobial					465:477	antimicrobial	465:477	antimicrobial	465:477	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	46	theme	nanoparticles	632:644	arg1	biosensing					453:462	the biosensing	449:462	the biosensing	449:462	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	46	theme	nanoparticles	632:644	arg1	embryotoxicity					483:496	embryotoxicity	483:496	embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites	483:688	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	46	theme	nanoparticles	632:644	arg1	NCs					697:699	Cs/Ag NCs	691:699	Cs/Ag NCs	691:699	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	47	theme	nanoparticles	522:534	arg1	antimicrobial					465:477	antimicrobial	465:477	antimicrobial	465:477	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	47	theme	nanoparticles	522:534	arg1	biosensing					453:462	the biosensing	449:462	the biosensing	449:462	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	47	theme	nanoparticles	522:534	arg1	embryotoxicity					483:496	embryotoxicity	483:496	embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites	483:688	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	47	theme	nanoparticles	522:534	arg1	NCs					697:699	Cs/Ag NCs	691:699	Cs/Ag NCs	691:699	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	7	48	theme	Gram	1317:1320	arg1	bacteria					1342:1349	Gram negative pathogenic bacteria	1317:1349	Gram negative pathogenic bacteria	1317:1349	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	6	49	theme	pronounced	1128:1137	arg1	properties					1147:1156	pronounced sensing properties	1128:1156	pronounced sensing properties	1128:1156	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	3	50	theme	selenium	546:553	arg1	NPs					573:575	Se NPs	570:575	Se NPs	570:575	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	50	theme	selenium	546:553	arg1	nanoparticles					555:567	selenium nanoparticles	546:567	selenium nanoparticles (Se NPs)	546:576	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	0	51	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	7	52	theme	NCs	1305:1307	arg1	activity					1264:1271	the effective inhibitory activity	1239:1271	the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria	1239:1349	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	1	53	theme	biological	279:288	arg1	fields					290:295	biological fields	279:295	biological fields	279:295	Hydrogen peroxide (H2O2) is widely used in various industries and biological fields.
35504473	10	54	theme	good	1601:1604	arg1	response					1606:1613	good response	1601:1613	good response in H2O2 sensing than the Cs/Ag NCs	1601:1648	However, Cs/Se NCs did not show good response in H2O2 sensing than the Cs/Ag NCs, implying the biocompatibility of Cs/Ag NCs.
35504473	2	55	theme	water	329:333	arg1	resources					335:343	water resources	329:343	water resources	329:343	H2O2 rapidly contaminants with water resources and hence simple detection process is highly wanted in various fields.
35504473	3	56	theme	nanocomposites	597:610	arg1	antimicrobial					465:477	antimicrobial	465:477	antimicrobial	465:477	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	56	theme	nanocomposites	597:610	arg1	biosensing					453:462	the biosensing	449:462	the biosensing	449:462	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	56	theme	nanocomposites	597:610	arg1	embryotoxicity					483:496	embryotoxicity	483:496	embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites	483:688	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	56	theme	nanocomposites	597:610	arg1	NCs					697:699	Cs/Ag NCs	691:699	Cs/Ag NCs	691:699	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	1	57	theme	Hydrogen	213:220	arg1	H2O2					232:235	H2O2	232:235	H2O2	232:235	Hydrogen peroxide (H2O2) is widely used in various industries and biological fields.
35504473	1	57	theme	Hydrogen	213:220	arg1	peroxide					222:229	Hydrogen peroxide	213:229	Hydrogen peroxide (H2O2)	213:236	Hydrogen peroxide (H2O2) is widely used in various industries and biological fields.
35504473	2	58	theme	simple	355:360	arg1	process					372:378	hence simple detection process	349:378	hence simple detection process	349:378	H2O2 rapidly contaminants with water resources and hence simple detection process is highly wanted in various fields.
35504473	5	59	theme	microscopic	877:887	arg1	techniques					889:898	various spectroscopic and microscopic techniques	851:898	various spectroscopic and microscopic techniques	851:898	The physico-chemical properties of as-synthesized nanomaterials were confirmed by various spectroscopic and microscopic techniques.
35504473	0	60	theme	nanoparticles	163:175	arg1	assessment					23:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	0	60	theme	nanoparticles	163:175	arg1	potential					49:57	biosensing potential	38:57	biosensing potential	38:57	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	7	61	theme	effective	1243:1251	arg1	activity					1264:1271	the effective inhibitory activity	1239:1271	the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria	1239:1349	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	0	62	theme	chitosan/silver	181:195	arg1	nanocomposites					197:210	chitosan/silver nanocomposites	181:210	chitosan/silver nanocomposites	181:210	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	3	63	theme	Cs/Se	613:617	arg1	nanocomposites					597:610	chitosan/selenium nanocomposites	579:610	chitosan/selenium nanocomposites (Cs/Se NCs)	579:622	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	63	theme	Cs/Se	613:617	arg1	NCs					619:621	Cs/Se NCs	613:621	Cs/Se NCs	613:621	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	6	64	theme	chronoamperometry	1070:1086	arg1	voltammetry					1003:1013	cyclic voltammetry	996:1013	cyclic voltammetry (CV)	996:1018	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	64	theme	chronoamperometry	1070:1086	arg1	methods					1093:1099	chronoamperometry (CA) methods	1070:1099	chronoamperometry (CA) methods	1070:1099	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	11	65	theme	biosensors	1849:1858	arg1	detection					1812:1820	the detection	1808:1820	the detection of environmental pollutant biosensors	1808:1858	Overall, the obtained results clearly suggest that Cs/Ag NCs could be suitable for dual applications such as for the detection of environmental pollutant biosensors and for biomedical research.
35504473	7	66	theme	as-prepared	1276:1286	arg1	NPs					1291:1293	as-prepared Ag NPs	1276:1293	as-prepared Ag NPs	1276:1293	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	3	67	theme	silver	625:630	arg1	nanoparticles					632:644	silver nanoparticles	625:644	silver nanoparticles (Ag NPs)	625:653	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	67	theme	silver	625:630	arg1	NPs					650:652	Ag NPs	647:652	Ag NPs	647:652	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	7	68	theme	inhibitory	1253:1262	arg1	activity					1264:1271	the effective inhibitory activity	1239:1271	the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria	1239:1349	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	9	69	theme	matrix	1561:1566	arg1	presence					1546:1553	the presence	1542:1553	the presence of Cs matrix	1542:1566	Intriguingly, the embryo treated with Cs/Se NCs and Cs/Ag NCs significantly reduced the toxicity in the presence of Cs matrix.
35504473	0	70	theme	nanoparticles	107:119	arg1	assessment					23:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment	0:32	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	0	70	theme	nanoparticles	107:119	arg1	potential					49:57	biosensing potential	38:57	biosensing potential	38:57	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	6	71	theme	impedance	1037:1045	arg1	spectroscopy					1047:1058	electrochemical impedance spectroscopy	1021:1058	electrochemical impedance spectroscopy (EIS)	1021:1064	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	71	theme	impedance	1037:1045	arg1	voltammetry					1003:1013	cyclic voltammetry	996:1013	cyclic voltammetry (CV)	996:1018	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	71	theme	impedance	1037:1045	arg1	EIS					1061:1063	EIS	1061:1063	EIS	1061:1063	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	0	72	theme	chitosan/selenium	122:138	arg1	nanocomposites					140:153	chitosan/selenium nanocomposites	122:153	chitosan/selenium nanocomposites	122:153	In vitro toxicological assessment and biosensing potential of bioinspired chitosan nanoparticles, selenium nanoparticles, chitosan/selenium nanocomposites, silver nanoparticles and chitosan/silver nanocomposites.
35504473	6	73	theme	Cs/Ag	1111:1115	arg1	NCs					1117:1119	Cs/Ag NCs	1111:1119	Cs/Ag NCs	1111:1119	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	8	74	theme	Ag	1409:1410	arg1	NPs					1412:1414	Ag NPs	1409:1414	Ag NPs	1409:1414	The highest embryotoxicity was recorded at 0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs.
35504473	7	75	theme	results	1210:1216	arg1	mode					1176:1179	the mode	1172:1179	the mode of antibacterial interaction results	1172:1216	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	5	76	theme	spectroscopic	859:871	arg1	techniques					889:898	various spectroscopic and microscopic techniques	851:898	various spectroscopic and microscopic techniques	851:898	The physico-chemical properties of as-synthesized nanomaterials were confirmed by various spectroscopic and microscopic techniques.
35504473	5	77	theme	various	851:857	arg1	techniques					889:898	various spectroscopic and microscopic techniques	851:898	various spectroscopic and microscopic techniques	851:898	The physico-chemical properties of as-synthesized nanomaterials were confirmed by various spectroscopic and microscopic techniques.
35504473	10	78	theme	NCs	1690:1692	arg1	biocompatibility					1664:1679	the biocompatibility	1660:1679	the biocompatibility of Cs/Ag NCs	1660:1692	However, Cs/Se NCs did not show good response in H2O2 sensing than the Cs/Ag NCs, implying the biocompatibility of Cs/Ag NCs.
35504473	5	79	theme	as-synthesized	804:817	arg1	nanomaterials					819:831	as-synthesized nanomaterials	804:831	as-synthesized nanomaterials	804:831	The physico-chemical properties of as-synthesized nanomaterials were confirmed by various spectroscopic and microscopic techniques.
35504473	6	80	theme	peroxide	919:926	arg1	properties					943:952	hydrogen peroxide (H2O2) sensing properties	910:952	hydrogen peroxide (H2O2) sensing properties	910:952	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	81	theme	CA	1089:1090	arg1	voltammetry					1003:1013	cyclic voltammetry	996:1013	cyclic voltammetry (CV)	996:1018	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	6	81	theme	CA	1089:1090	arg1	methods					1093:1099	chronoamperometry (CA) methods	1070:1099	chronoamperometry (CA) methods	1070:1099	Further, hydrogen peroxide (H2O2) sensing properties and their sensitivities were confirmed by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and chronoamperometry (CA) methods, in which Cs/Ag NCs showed pronounced sensing properties.
35504473	8	82	theme	NPs	1437:1439	arg1	1.56 μg/ml					1420:1429	1.56 μg/ml	1420:1429	1.56 μg/ml of Se NPs	1420:1439	The highest embryotoxicity was recorded at 0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs.
35504473	8	82	theme	NPs	1437:1439	arg1	NPs					1412:1414	Ag NPs	1409:1414	Ag NPs	1409:1414	The highest embryotoxicity was recorded at 0.19 μg/ml of Ag NPs and 1.56 μg/ml of Se NPs.
35504473	7	83	theme	negative	1322:1329	arg1	bacteria					1342:1349	Gram negative pathogenic bacteria	1317:1349	Gram negative pathogenic bacteria	1317:1349	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	2	84	theme	various	400:406	arg1	fields					408:413	various fields	400:413	various fields	400:413	H2O2 rapidly contaminants with water resources and hence simple detection process is highly wanted in various fields.
35504473	3	85	theme	Cs/Ag	691:695	arg1	antimicrobial					465:477	antimicrobial	465:477	antimicrobial	465:477	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	85	theme	Cs/Ag	691:695	arg1	NCs					697:699	Cs/Ag NCs	691:699	Cs/Ag NCs	691:699	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	11	86	theme	biomedical	1868:1877	arg1	research					1879:1886	biomedical research	1868:1886	biomedical research	1868:1886	Overall, the obtained results clearly suggest that Cs/Ag NCs could be suitable for dual applications such as for the detection of environmental pollutant biosensors and for biomedical research.
35504473	3	87	theme	nanocomposites	675:688	arg1	antimicrobial					465:477	antimicrobial	465:477	antimicrobial	465:477	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	87	theme	nanocomposites	675:688	arg1	biosensing					453:462	the biosensing	449:462	the biosensing	449:462	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	87	theme	nanocomposites	675:688	arg1	embryotoxicity					483:496	embryotoxicity	483:496	embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites	483:688	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	87	theme	nanocomposites	675:688	arg1	NCs					697:699	Cs/Ag NCs	691:699	Cs/Ag NCs	691:699	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	2	88	theme	detection	362:370	arg1	process					372:378	hence simple detection process	349:378	hence simple detection process	349:378	H2O2 rapidly contaminants with water resources and hence simple detection process is highly wanted in various fields.
35504473	1	89	theme	various	256:262	arg1	industries					264:273	various industries	256:273	various industries	256:273	Hydrogen peroxide (H2O2) is widely used in various industries and biological fields.
35504473	7	90	theme	Cs/Ag	1299:1303	arg1	NCs					1305:1307	Cs/Ag NCs	1299:1307	Cs/Ag NCs	1299:1307	In addition, the mode of antibacterial interaction results clearly demonstrated the effective inhibitory activity of as-prepared Ag NPs and Cs/Ag NCs against Gram negative pathogenic bacteria.
35504473	10	91	theme	Cs/Se	1578:1582	arg1	NCs					1584:1586	Cs/Se NCs	1578:1586	Cs/Se NCs	1578:1586	However, Cs/Se NCs did not show good response in H2O2 sensing than the Cs/Ag NCs, implying the biocompatibility of Cs/Ag NCs.
35504473	3	92	theme	nanoparticles	555:567	arg1	antimicrobial					465:477	antimicrobial	465:477	antimicrobial	465:477	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	92	theme	nanoparticles	555:567	arg1	biosensing					453:462	the biosensing	449:462	the biosensing	449:462	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	92	theme	nanoparticles	555:567	arg1	embryotoxicity					483:496	embryotoxicity	483:496	embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites	483:688	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	3	92	theme	nanoparticles	555:567	arg1	NCs					697:699	Cs/Ag NCs	691:699	Cs/Ag NCs	691:699	The present study was focused on the biosensing, antimicrobial and embryotoxicity of bioinspired chitosan nanoparticles (Cs NPs), selenium nanoparticles (Se NPs), chitosan/selenium nanocomposites (Cs/Se NCs), silver nanoparticles (Ag NPs) and chitosan/silver nanocomposites (Cs/Ag NCs) synthesized using the aqueous Cucurbita pepo Linn.
35504473	11	93	theme	pollutant	1839:1847	arg1	biosensors					1849:1858	environmental pollutant biosensors	1825:1858	environmental pollutant biosensors	1825:1858	Overall, the obtained results clearly suggest that Cs/Ag NCs could be suitable for dual applications such as for the detection of environmental pollutant biosensors and for biomedical research.
36696678	5	0	theme	α-N-acetylglucosaminidase	758:782	arg1	levels					738:743	the levels	734:743	the levels of defective α-N-acetylglucosaminidase	734:782	Particularly, we found that they increase the levels of defective α-N-acetylglucosaminidase and correct its proper sorting toward the lysosomal compartment.
36696678	6	1	theme	glycosyltransferases	945:964	arg1	levels					925:930	protein levels	917:930	protein levels of exostosin glycosyltransferases	917:964	Furthermore, l-iminosugars reduce HS accumulation by downregulating protein levels of exostosin glycosyltransferases.
36696678	4	2	theme	substrate	563:571	arg1	storage					573:579	substrate storage	563:579	substrate storage	563:579	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	3	3	theme	approved	430:437	arg1	treatments					439:448	no approved treatments	427:448	no approved treatments for Sanfilippo disease	427:471	To date, no approved treatments for Sanfilippo disease exist.
36696678	6	4	theme	exostosin	935:943	arg1	glycosyltransferases					945:964	exostosin glycosyltransferases	935:964	exostosin glycosyltransferases	935:964	Furthermore, l-iminosugars reduce HS accumulation by downregulating protein levels of exostosin glycosyltransferases.
36696678	7	5	theme	incurable	1172:1180	arg1	disease					1182:1188	such incurable disease	1167:1188	such incurable disease	1167:1188	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	2	6	theme	other	267:271	arg1	compartments					282:293	other cellular compartments	267:293	other cellular compartments	267:293	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	4	7	theme	lysosomal	585:593	arg1	dysfunctions					595:606	lysosomal dysfunctions	585:606	lysosomal dysfunctions	585:606	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	7	8	theme	disease	1182:1188	arg1	treatment					1154:1162	the treatment	1150:1162	the treatment of such incurable disease	1150:1188	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	7	9	theme	pharmacological	1006:1020	arg1	potential					1022:1030	an interesting pharmacological potential	991:1030	an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome	991:1076	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	2	10	theme	clinical	364:371	arg1	symptoms					373:380	clinical symptoms	364:380	clinical symptoms including severe neurodegeneration	364:415	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	2	10	theme	clinical	364:371	arg1	neurodegeneration					399:415	severe neurodegeneration	392:415	severe neurodegeneration	392:415	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	6	11	theme	protein	917:923	arg1	levels					925:930	protein levels	917:930	protein levels of exostosin glycosyltransferases	917:964	Furthermore, l-iminosugars reduce HS accumulation by downregulating protein levels of exostosin glycosyltransferases.
36696678	1	12	theme	Sanfilippo	77:86	arg1	syndrome					88:95	Sanfilippo syndrome	77:95	Sanfilippo syndrome	77:95	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	1	13	theme	decreased	159:167	arg1	activity					169:176	decreased activity	159:176	decreased activity	159:176	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	7	14	theme	interesting	994:1004	arg1	potential					1022:1030	an interesting pharmacological potential	991:1030	an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome	991:1076	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	4	15	theme	Sanfilippo	670:679	arg1	subtype					683:689	Sanfilippo B subtype	670:689	Sanfilippo B subtype	670:689	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	0	16	theme	N-Substituted	0:12	arg1	l-Iminosugars					14:26	N-Substituted l-Iminosugars	0:26	N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome.	0:75	N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome.
36696678	1	17	dep	lack	151:154	arg1	the					147:149	the	147:149	the	147:149	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	2	18	theme	wide	350:353	arg1	range					355:359	a wide range	348:359	a wide range of clinical symptoms including severe neurodegeneration	348:415	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	2	18	theme	wide	350:353	arg1	neurodegeneration					399:415	severe neurodegeneration	392:415	severe neurodegeneration	392:415	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	5	19	theme	lysosomal	826:834	arg1	compartment					836:846	the lysosomal compartment	822:846	the lysosomal compartment	822:846	Particularly, we found that they increase the levels of defective α-N-acetylglucosaminidase and correct its proper sorting toward the lysosomal compartment.
36696678	4	20	theme	l-iminosugars	525:537	arg1	ability					500:506	the ability	496:506	the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype	496:689	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	7	21	theme	approaches	1135:1144	arg1	development					1102:1112	the development	1098:1112	the development of novel therapeutic approaches for the treatment of such incurable disease	1098:1188	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	5	22	theme	proper	800:805	arg1	sorting					807:813	its proper sorting	796:813	its proper sorting toward the lysosomal compartment	796:846	Particularly, we found that they increase the levels of defective α-N-acetylglucosaminidase and correct its proper sorting toward the lysosomal compartment.
36696678	1	23	theme	enzymes	181:187	arg1	activity					169:176	decreased activity	159:176	decreased activity	159:176	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	1	23	theme	enzymes	181:187	arg1	lack					151:154	lack	151:154	lack	151:154	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	2	24	dep	tissue	306:311	arg1	dysfunctions					323:334	dysfunctions	323:334	dysfunctions	323:334	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	2	25	theme	cellular	273:280	arg1	compartments					282:293	other cellular compartments	267:293	other cellular compartments	267:293	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	2	26	from	accumulation	237:248	arg1	compartments					282:293	other cellular compartments	267:293	other cellular compartments	267:293	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	2	26	from	accumulation	237:248	arg1	lysosomes					253:261	lysosomes	253:261	lysosomes	253:261	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	2	27	theme	severe	392:397	arg1	neurodegeneration					399:415	severe neurodegeneration	392:415	severe neurodegeneration	392:415	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	7	28	theme	novel	1117:1121	arg1	approaches					1135:1144	novel therapeutic approaches	1117:1144	novel therapeutic approaches for the treatment of such incurable disease	1117:1188	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	4	29	theme	neuronal	643:650	arg1	model					661:665	a neuronal cellular model	641:665	a neuronal cellular model of Sanfilippo B subtype	641:689	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	4	30	theme	subtype	683:689	arg1	model					661:665	a neuronal cellular model	641:665	a neuronal cellular model of Sanfilippo B subtype	641:689	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	7	31	from	potential	1022:1030	arg1	syndrome					1069:1076	Sanfilippo syndrome	1058:1076	Sanfilippo syndrome	1058:1076	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	7	32	theme	therapeutic	1123:1133	arg1	approaches					1135:1144	novel therapeutic approaches	1117:1144	novel therapeutic approaches for the treatment of such incurable disease	1117:1188	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	6	33	theme	HS	883:884	arg1	accumulation					886:897	HS accumulation	883:897	HS accumulation	883:897	Furthermore, l-iminosugars reduce HS accumulation by downregulating protein levels of exostosin glycosyltransferases.
36696678	2	34	theme	symptoms	373:380	arg1	range					355:359	a wide range	348:359	a wide range of clinical symptoms including severe neurodegeneration	348:415	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	2	34	theme	symptoms	373:380	arg1	neurodegeneration					399:415	severe neurodegeneration	392:415	severe neurodegeneration	392:415	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	0	35	theme	Type	60:63	arg1	Syndrome					67:74	Sanfilippo Type B Syndrome	49:74	Sanfilippo Type B Syndrome	49:74	N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome.
36696678	5	36	theme	defective	748:756	arg1	α-N-acetylglucosaminidase					758:782	defective α-N-acetylglucosaminidase	748:782	defective α-N-acetylglucosaminidase	748:782	Particularly, we found that they increase the levels of defective α-N-acetylglucosaminidase and correct its proper sorting toward the lysosomal compartment.
36696678	0	37	theme	Sanfilippo	49:58	arg1	Syndrome					67:74	Sanfilippo Type B Syndrome	49:74	Sanfilippo Type B Syndrome	49:74	N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome.
36696678	4	38	theme	N-substituted	511:523	arg1	l-iminosugars					525:537	N-substituted l-iminosugars	511:537	N-substituted l-iminosugars	511:537	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	1	39	theme	genetic	123:129	arg1	diseases					131:138	four genetic diseases	118:138	four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism	118:231	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	1	40	theme	sulfate	209:215	arg1	catabolism					222:231	heparan sulfate (HS) catabolism	201:231	heparan sulfate (HS) catabolism	201:231	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	2	41	theme	HS	234:235	arg1	accumulation					237:248	HS accumulation	234:248	HS accumulation in lysosomes and other cellular compartments	234:293	HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to a wide range of clinical symptoms including severe neurodegeneration.
36696678	7	42	theme	Sanfilippo	1058:1067	arg1	syndrome					1069:1076	Sanfilippo syndrome	1058:1076	Sanfilippo syndrome	1058:1076	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	1	43	theme	diseases	131:138	arg1	diseases					131:138	four genetic diseases	118:138	four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism	118:231	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	1	43	theme	diseases	131:138	arg1	group					109:113	a group	107:113	a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism	107:231	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	3	44	theme	Sanfilippo	454:463	arg1	disease					465:471	Sanfilippo disease	454:471	Sanfilippo disease	454:471	To date, no approved treatments for Sanfilippo disease exist.
36696678	0	45	theme	Syndrome	67:74	arg1	Treatment					36:44	the Treatment	32:44	the Treatment of Sanfilippo Type B Syndrome	32:74	N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome.
36696678	1	46	theme	heparan	201:207	arg1	HS					218:219	HS	218:219	HS	218:219	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	1	46	theme	heparan	201:207	arg1	sulfate					209:215	heparan sulfate	201:215	heparan sulfate (HS) catabolism	201:231	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	1	47	theme	due	140:142	arg1	diseases					131:138	four genetic diseases	118:138	four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism	118:231	Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism.
36696678	4	48	theme	cellular	652:659	arg1	model					661:665	a neuronal cellular model	641:665	a neuronal cellular model of Sanfilippo B subtype	641:689	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	0	49	theme	B	65:65	arg1	Syndrome					67:74	Sanfilippo Type B Syndrome	49:74	Sanfilippo Type B Syndrome	49:74	N-Substituted l-Iminosugars for the Treatment of Sanfilippo Type B Syndrome.
36696678	7	50	theme	glycomimetics	1041:1053	arg1	potential					1022:1030	an interesting pharmacological potential	991:1030	an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome	991:1076	These results highlight an interesting pharmacological potential of these glycomimetics in Sanfilippo syndrome, paving the way for the development of novel therapeutic approaches for the treatment of such incurable disease.
36696678	4	51	theme	B	681:681	arg1	subtype					683:689	Sanfilippo B subtype	670:689	Sanfilippo B subtype	670:689	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
36696678	4	52	theme	Sanfilippo	611:620	arg1	fibroblasts					622:632	Sanfilippo fibroblasts	611:632	Sanfilippo fibroblasts	611:632	Here, we report the ability of N-substituted l-iminosugars to significantly reduce substrate storage and lysosomal dysfunctions in Sanfilippo fibroblasts and in a neuronal cellular model of Sanfilippo B subtype.
35988981	0	0	theme	local	89:93	arg1	therapy					102:108	a safe dual-drug local cancer therapy	72:108	a safe dual-drug local cancer therapy approach	72:117	Smart and eco-friendly N-isopropylacrylamide and cellulose hydrogels as a safe dual-drug local cancer therapy approach.
35988981	2	1	theme	citric	380:385	arg1	acid					387:390	citric acid	380:390	citric acid	380:390	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	2	2	theme	hydrophobic	494:504	arg1	drugs					520:524	hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs	464:524	hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs	464:524	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	0	3	theme	therapy	102:108	arg1	approach					110:117	a safe dual-drug local cancer therapy approach	72:117	a safe dual-drug local cancer therapy approach	72:117	Smart and eco-friendly N-isopropylacrylamide and cellulose hydrogels as a safe dual-drug local cancer therapy approach.
35988981	7	4	theme	eco-friendly	1079:1090	arg1	composition					1092:1102	its natural and eco-friendly composition	1063:1102	its natural and eco-friendly composition	1063:1102	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	2	5	theme	biodegradable	294:306	arg1	HG					331:332	a biodegradable and multicompartmental HG	292:332	a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate	292:418	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	0	6	theme	cancer	95:100	arg1	therapy					102:108	a safe dual-drug local cancer therapy	72:108	a safe dual-drug local cancer therapy approach	72:117	Smart and eco-friendly N-isopropylacrylamide and cellulose hydrogels as a safe dual-drug local cancer therapy approach.
35988981	7	7	theme	tumors	1194:1199	arg1	treatment					1156:1164	the local treatment	1146:1164	the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy	1146:1239	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	7	8	theme	adjuvant	1224:1231	arg1	therapy					1233:1239	pre-surgical adjuvant therapy	1211:1239	pre-surgical adjuvant therapy	1211:1239	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	6	9	theme	safety	997:1002	arg1	profile					1004:1010	safety profile	997:1010	safety profile	997:1010	Moreover, HG demonstrated a proper injectability, in situ prevalence, and safety profile in vivo.
35988981	5	10	theme	cell	911:914	arg1	lines					916:920	both cell lines	906:920	both cell lines	906:920	This low release was sufficient to cause cell death in both cell lines.
35988981	7	11	theme	natural	1067:1073	arg1	composition					1092:1102	its natural and eco-friendly composition	1063:1102	its natural and eco-friendly composition	1063:1102	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	4	12	theme	doxorubicin	792:802	arg1	%					811:811	30 %	808:811	30 % of niclosamide released after 1 week	808:848	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	4	12	theme	doxorubicin	792:802	arg1	%					787:787	only 4 %	780:787	only 4 % of doxorubicin	780:802	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	4	12	theme	doxorubicin	792:802	arg1	niclosamide					816:826	niclosamide	816:826	niclosamide released after 1 week	816:848	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	4	12	theme	doxorubicin	792:802	arg1	doxorubicin					792:802	doxorubicin	792:802	doxorubicin	792:802	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	3	13	from	biocompatibility	647:662	arg1	cells					717:721	colon and ovarian cancer cells	692:721	colon and ovarian cancer cells	692:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	1	14	theme	thermo-responsive	168:184	arg1	HG					197:198	HG	197:198	HG	197:198	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	1	14	theme	thermo-responsive	168:184	arg1	hydrogels					186:194	thermo-responsive hydrogels	168:194	thermo-responsive hydrogels (HG)	168:199	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	2	15	theme	drugs	520:524	arg1	release					453:459	the controlled release	438:459	the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs	438:524	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	3	16	theme	ideal	553:557	arg1	properties					559:568	ideal properties	553:568	ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells	553:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	1	17	theme	hydrogels	186:194	arg1	injections					154:163	in situ injections	146:163	in situ injections of thermo-responsive hydrogels (HG)	146:199	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	2	18	theme	hydrophilic	464:474	arg1	drugs					520:524	hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs	464:524	hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs	464:524	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	6	19	theme	proper	951:956	arg1	injectability					958:970	a proper injectability	949:970	a proper injectability	949:970	Moreover, HG demonstrated a proper injectability, in situ prevalence, and safety profile in vivo.
35988981	2	20	dep	hydrophilic	464:474	arg1	doxorubicin					477:487	doxorubicin	477:487	doxorubicin	477:487	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	3	21	theme	rheological	603:613	arg1	behavior					615:622	rheological behavior	603:622	rheological behavior	603:622	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	2	22	theme	ceric	397:401	arg1	nitrate					412:418	ceric ammonium nitrate	397:418	ceric ammonium nitrate	397:418	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	0	23	theme	Smart	0:4	arg1	N-isopropylacrylamide					23:43	Smart and eco-friendly N-isopropylacrylamide	0:43	Smart and eco-friendly N-isopropylacrylamide	0:43	Smart and eco-friendly N-isopropylacrylamide and cellulose hydrogels as a safe dual-drug local cancer therapy approach.
35988981	6	24	theme	in	973:974	arg1	prevalence					981:990	in situ prevalence	973:990	in situ prevalence	973:990	Moreover, HG demonstrated a proper injectability, in situ prevalence, and safety profile in vivo.
35988981	5	25	theme	low	856:858	arg1	release					860:866	This low release	851:866	This low release	851:866	This low release was sufficient to cause cell death in both cell lines.
35988981	1	26	dep	in	146:147	arg1	situ					149:152	situ	149:152	situ	149:152	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	2	27	theme	controlled	442:451	arg1	release					453:459	the controlled release	438:459	the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs	438:524	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	5	28	theme	cell	892:895	arg1	death					897:901	cell death	892:901	cell death	892:901	This low release was sufficient to cause cell death in both cell lines.
35988981	2	29	theme	multicompartmental	312:329	arg1	HG					331:332	a biodegradable and multicompartmental HG	292:332	a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate	292:418	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	7	30	theme	HG	1034:1035	arg1	properties					1037:1046	the HG properties	1030:1046	the HG properties	1030:1046	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	3	31	theme	colon	692:696	arg1	cells					717:721	colon and ovarian cancer cells	692:721	colon and ovarian cancer cells	692:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	3	32	theme	release	630:636	arg1	profile					638:644	drug release profile	625:644	drug release profile	625:644	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	3	33	from	profile	638:644	arg1	cells					717:721	colon and ovarian cancer cells	692:721	colon and ovarian cancer cells	692:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	1	34	theme	several	208:214	arg1	advantages					216:225	several advantages	208:225	several advantages	208:225	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	7	35	theme	efficient	1123:1131	arg1	platform					1133:1140	a safe and efficient platform	1112:1140	a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy	1112:1239	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	3	36	theme	biological	669:678	arg1	activity					680:687	biological activity	669:687	biological activity	669:687	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	4	37	theme	niclosamide	816:826	arg1	%					811:811	30 %	808:811	30 % of niclosamide released after 1 week	808:848	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	4	37	theme	niclosamide	816:826	arg1	%					787:787	only 4 %	780:787	only 4 % of doxorubicin	780:802	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	4	37	theme	niclosamide	816:826	arg1	niclosamide					816:826	niclosamide	816:826	niclosamide released after 1 week	816:848	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	4	37	theme	niclosamide	816:826	arg1	doxorubicin					792:802	doxorubicin	792:802	doxorubicin	792:802	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	7	38	theme	non-resectable	1169:1182	arg1	tumors					1184:1189	non-resectable tumors	1169:1189	non-resectable tumors	1169:1189	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	1	39	theme	Local	120:124	arg1	treatment					133:141	Local cancer treatment	120:141	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG)	120:199	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	7	40	theme	safe	1114:1117	arg1	platform					1133:1140	a safe and efficient platform	1112:1140	a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy	1112:1239	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	2	41	dep	hydrophobic	494:504	arg1	niclosamide					507:517	niclosamide	507:517	niclosamide	507:517	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	3	42	theme	cancer	710:715	arg1	cells					717:721	colon and ovarian cancer cells	692:721	colon and ovarian cancer cells	692:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	3	43	theme	drug	625:628	arg1	profile					638:644	drug release profile	625:644	drug release profile	625:644	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	7	44	theme	tumors	1184:1189	arg1	treatment					1156:1164	the local treatment	1146:1164	the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy	1146:1239	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	1	45	theme	cancer	126:131	arg1	treatment					133:141	Local cancer treatment	120:141	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG)	120:199	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	3	46	from	thermo-responsiveness	580:600	arg1	cells					717:721	colon and ovarian cancer cells	692:721	colon and ovarian cancer cells	692:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	4	47	theme	release	760:766	arg1	rate					768:771	drugs release rate	754:771	drugs release rate	754:771	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	0	48	dep	safe	74:77	arg1	dual-drug					79:87	dual-drug	79:87	dual-drug	79:87	Smart and eco-friendly N-isopropylacrylamide and cellulose hydrogels as a safe dual-drug local cancer therapy approach.
35988981	3	49	from	behavior	615:622	arg1	cells					717:721	colon and ovarian cancer cells	692:721	colon and ovarian cancer cells	692:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	6	50	dep	in	973:974	arg1	situ					976:979	situ	976:979	situ	976:979	Moreover, HG demonstrated a proper injectability, in situ prevalence, and safety profile in vivo.
35988981	4	51	theme	drugs	754:758	arg1	rate					768:771	drugs release rate	754:771	drugs release rate	754:771	Cellulose was found to retard drugs release rate, being only 4 % of doxorubicin and 30 % of niclosamide released after 1 week.
35988981	1	52	theme	conventional	232:243	arg1	treatments					266:275	conventional systemic anti-cancer treatments	232:275	conventional systemic anti-cancer treatments	232:275	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	0	53	theme	safe	74:77	arg1	therapy					102:108	a safe dual-drug local cancer therapy	72:108	a safe dual-drug local cancer therapy approach	72:117	Smart and eco-friendly N-isopropylacrylamide and cellulose hydrogels as a safe dual-drug local cancer therapy approach.
35988981	3	54	theme	ovarian	702:708	arg1	cancer					710:715	ovarian cancer	702:715	ovarian cancer	702:715	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	1	55	theme	in	146:147	arg1	injections					154:163	in situ injections	146:163	in situ injections of thermo-responsive hydrogels (HG)	146:199	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	2	56	theme	ammonium	403:410	arg1	nitrate					412:418	ceric ammonium nitrate	397:418	ceric ammonium nitrate	397:418	In this work, a biodegradable and multicompartmental HG composed of N-isopropylacrylamide, cellulose, citric acid, and ceric ammonium nitrate was developed for the controlled release of hydrophilic (doxorubicin) and hydrophobic (niclosamide) drugs.
35988981	1	57	theme	systemic	245:252	arg1	treatments					266:275	conventional systemic anti-cancer treatments	232:275	conventional systemic anti-cancer treatments	232:275	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35988981	7	58	theme	pre-surgical	1211:1222	arg1	therapy					1233:1239	pre-surgical adjuvant therapy	1211:1239	pre-surgical adjuvant therapy	1211:1239	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	0	59	theme	eco-friendly	10:21	arg1	N-isopropylacrylamide					23:43	Smart and eco-friendly N-isopropylacrylamide	0:43	Smart and eco-friendly N-isopropylacrylamide	0:43	Smart and eco-friendly N-isopropylacrylamide and cellulose hydrogels as a safe dual-drug local cancer therapy approach.
35988981	3	60	from	activity	680:687	arg1	cells					717:721	colon and ovarian cancer cells	692:721	colon and ovarian cancer cells	692:721	The formulation presented ideal properties regarding thermo-responsiveness, rheological behavior, drug release profile, biocompatibility, and biological activity in colon and ovarian cancer cells.
35988981	7	61	theme	local	1150:1154	arg1	treatment					1156:1164	the local treatment	1146:1164	the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy	1146:1239	Overall, the HG properties, together with its natural and eco-friendly composition, create a safe and efficient platform for the local treatment of non-resectable tumors or tumors requiring pre-surgical adjuvant therapy.
35988981	1	62	theme	anti-cancer	254:264	arg1	treatments					266:275	conventional systemic anti-cancer treatments	232:275	conventional systemic anti-cancer treatments	232:275	Local cancer treatment by in situ injections of thermo-responsive hydrogels (HG) offers several advantages over conventional systemic anti-cancer treatments.
35506498	7	0	theme	microorganisms	1060:1073	arg1	composition					1030:1040	composition	1030:1040	composition	1030:1040	Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment.
35506498	7	0	theme	microorganisms	1060:1073	arg1	structures					1015:1024	structures	1015:1024	structures	1015:1024	Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment.
35506498	2	1	theme	kg-1	319:322	arg1	sulfasalazine					324:336	200 mg kg-1 sulfasalazine	312:336	200 mg kg-1 sulfasalazine	312:336	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	5	2	theme	proteins	834:841	arg1	expression					805:814	expression	805:814	expression of tight junction proteins (p < 0.05)	805:852	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	9	3	with	patients	1436:1443	arg1	disease					1469:1475	inflammatory bowel disease	1450:1475	inflammatory bowel disease	1450:1475	These findings provide new insights into dietary management for patients with inflammatory bowel disease.
35506498	5	4	theme	junction	825:832	arg1	proteins					834:841	tight junction proteins	819:841	tight junction proteins (p < 0.05)	819:852	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	5	4	theme	junction	825:832	arg1	<					846:846	p < 0.05	844:851	p < 0.05	844:851	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	2	5	theme	mg	316:317	arg1	sulfasalazine					324:336	200 mg kg-1 sulfasalazine	312:336	200 mg kg-1 sulfasalazine	312:336	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	8	6	theme	anti-inflammatory	1303:1319	arg1	bacteria					1321:1328	anti-inflammatory bacteria	1303:1328	anti-inflammatory bacteria	1303:1328	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	5	7	theme	tight	819:823	arg1	proteins					834:841	tight junction proteins	819:841	tight junction proteins (p < 0.05)	819:852	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	5	7	theme	tight	819:823	arg1	<					846:846	p < 0.05	844:851	p < 0.05	844:851	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	7	8	theme	KOS	1098:1100	arg1	treatment					1102:1110	KOS treatment	1098:1110	KOS treatment	1098:1110	Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment.
35506498	2	9	theme	mg	284:285	arg1	kg-1					287:290	200 mg kg-1	280:290	200 mg kg-1	280:290	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	4	10	theme	pathological	577:588	arg1	phenotype					566:574	phenotype	566:574	phenotype	566:574	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	4	10	theme	pathological	577:588	arg1	lesion					590:595	pathological lesion	577:595	pathological lesion of the colon	577:608	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	6	11	theme	colon	898:902	arg1	tissue					904:909	the colon tissue	894:909	the colon tissue of the colitis mice	894:929	The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal.
35506498	3	12	theme	KOS	499:501	arg1	intervention					483:494	the intervention	479:494	the intervention of KOS and sulfasalazine	479:519	The mice were then treated with drinking water containing 2.5% DSS for 9 days, while the intervention of KOS and sulfasalazine continued.
35506498	2	13	theme	200	280:282	arg1	mg					284:285	mg	284:285	mg	284:285	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	1	14	theme	oligosaccharides	155:170	arg1	effect					138:143	the protective effect	123:143	the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice	123:222	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	5	15	theme	colon	764:768	arg1	tissue					770:775	the colon tissue	760:775	the colon tissue	760:775	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	4	16	theme	cytokines	625:633	arg1	parameters					611:620	the phenotype, pathological lesion of the colon, parameters	562:620	the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota	562:652	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	7	17	theme	gut	982:984	arg1	microbiota					986:995	the gut microbiota	978:995	the gut microbiota	978:995	Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment.
35506498	8	18	dep	up-regulates	1273:1284	arg1	Akkermansiaceae					1354:1368	Akkermansiaceae	1354:1368	Akkermansiaceae	1354:1368	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	8	18	dep	up-regulates	1273:1284	arg1	Bifidobacteriaceae					1331:1348	Bifidobacteriaceae	1331:1348	Bifidobacteriaceae	1331:1348	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	2	19	theme	KOS	359:361	arg1	sulfasalazine					324:336	200 mg kg-1 sulfasalazine	312:336	200 mg kg-1 sulfasalazine	312:336	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	2	19	theme	KOS	359:361	arg1	KOS					307:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	2	19	theme	KOS	359:361	arg1	combination					344:354	a combination	342:354	a combination of KOS and sulfasalazine	342:379	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	0	20	from	colitis	57:63	arg1	mice					68:71	mice	68:71	mice	68:71	Konjac oligosaccharides attenuate DSS-induced ulcerative colitis in mice: mechanistic insights.
35506498	7	21	theme	microbiota	986:995	arg1	analysis					966:973	the analysis	962:973	the analysis of the gut microbiota	962:995	Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment.
35506498	5	22	theme	alleviated	726:735	arg1	lesion					750:755	alleviated pathological lesion	726:755	alleviated pathological lesion of the colon tissue	726:775	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	9	23	theme	new	1395:1397	arg1	insights					1399:1406	new insights	1395:1406	new insights into dietary management for patients with inflammatory bowel disease	1395:1475	These findings provide new insights into dietary management for patients with inflammatory bowel disease.
35506498	3	24	contain	containing	441:450	arg2	DSS					457:459	2.5% DSS	452:459	2.5% DSS for 9 days	452:470	The mice were then treated with drinking water containing 2.5% DSS for 9 days, while the intervention of KOS and sulfasalazine continued.
35506498	3	24	contain	containing	441:450	arg1	water					435:439	water	435:439	water containing 2.5% DSS for 9 days	435:470	The mice were then treated with drinking water containing 2.5% DSS for 9 days, while the intervention of KOS and sulfasalazine continued.
35506498	1	25	from	effect	138:143	arg1	disease					200:206	inflammatory bowel disease	181:206	inflammatory bowel disease in colitis mice	181:222	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	2	26	theme	mg	299:300	arg1	kg-1					302:305	600 mg kg-1	295:305	600 mg kg-1	295:305	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	9	27	theme	inflammatory	1450:1461	arg1	disease					1469:1475	inflammatory bowel disease	1450:1475	inflammatory bowel disease	1450:1475	These findings provide new insights into dietary management for patients with inflammatory bowel disease.
35506498	0	28	theme	Konjac	0:5	arg1	oligosaccharides					7:22	Konjac oligosaccharides	0:22	Konjac oligosaccharides	0:22	Konjac oligosaccharides attenuate DSS-induced ulcerative colitis in mice: mechanistic insights.
35506498	2	29	theme	600	295:297	arg1	mg					299:300	mg	299:300	mg	299:300	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	1	30	theme	inflammatory	181:192	arg1	disease					200:206	inflammatory bowel disease	181:206	inflammatory bowel disease in colitis mice	181:222	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	0	31	theme	DSS-induced	34:44	arg1	colitis					57:63	DSS-induced ulcerative colitis	34:63	DSS-induced ulcerative colitis in mice	34:71	Konjac oligosaccharides attenuate DSS-induced ulcerative colitis in mice: mechanistic insights.
35506498	6	32	theme	cytokines	881:889	arg1	normal					944:949	normal	944:949	normal	944:949	The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal.
35506498	6	32	theme	cytokines	881:889	arg1	level					859:863	The level	855:863	The level of inflammatory cytokines in the colon tissue of the colitis mice	855:929	The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal.
35506498	8	33	theme	bacteria	1321:1328	arg1	abundance					1290:1298	the abundance	1286:1298	the abundance of anti-inflammatory bacteria	1286:1328	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	1	34	theme	bowel	194:198	arg1	disease					200:206	inflammatory bowel disease	181:206	inflammatory bowel disease in colitis mice	181:222	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	4	35	theme	microbiota	643:652	arg1	parameters					611:620	the phenotype, pathological lesion of the colon, parameters	562:620	the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota	562:652	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	6	36	theme	inflammatory	868:879	arg1	cytokines					881:889	inflammatory cytokines	868:889	inflammatory cytokines	868:889	The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal.
35506498	9	37	theme	bowel	1463:1467	arg1	disease					1469:1475	inflammatory bowel disease	1450:1475	inflammatory bowel disease	1450:1475	These findings provide new insights into dietary management for patients with inflammatory bowel disease.
35506498	2	38	theme	kg-1	287:290	arg1	KOS					307:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	4	39	theme	experiment	550:559	arg1	end					539:541	the end	535:541	the end of the experiment	535:559	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	3	40	theme	%	455:455	arg1	DSS					457:459	2.5% DSS	452:459	2.5% DSS for 9 days	452:470	The mice were then treated with drinking water containing 2.5% DSS for 9 days, while the intervention of KOS and sulfasalazine continued.
35506498	8	41	theme	pro-inflammatory	1189:1204	arg1	bacteria					1206:1213	pro-inflammatory bacteria	1189:1213	pro-inflammatory bacteria	1189:1213	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	4	42	theme	phenotype	566:574	arg1	parameters					611:620	the phenotype, pathological lesion of the colon, parameters	562:620	the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota	562:652	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	2	43	theme	sulfasalazine	367:379	arg1	sulfasalazine					324:336	200 mg kg-1 sulfasalazine	312:336	200 mg kg-1 sulfasalazine	312:336	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	2	43	theme	sulfasalazine	367:379	arg1	KOS					307:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	2	43	theme	sulfasalazine	367:379	arg1	combination					344:354	a combination	342:354	a combination of KOS and sulfasalazine	342:379	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	0	44	theme	ulcerative	46:55	arg1	colitis					57:63	DSS-induced ulcerative colitis	34:63	DSS-induced ulcerative colitis in mice	34:71	Konjac oligosaccharides attenuate DSS-induced ulcerative colitis in mice: mechanistic insights.
35506498	5	45	theme	pathological	737:748	arg1	lesion					750:755	alleviated pathological lesion	726:755	alleviated pathological lesion of the colon tissue	726:775	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	1	46	theme	colitis	211:217	arg1	mice					219:222	colitis mice	211:222	colitis mice	211:222	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	8	47	theme	bacteria	1206:1213	arg1	abundance					1176:1184	the abundance	1172:1184	the abundance of pro-inflammatory bacteria	1172:1213	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	3	48	theme	sulfasalazine	507:519	arg1	intervention					483:494	the intervention	479:494	the intervention of KOS and sulfasalazine	479:519	The mice were then treated with drinking water containing 2.5% DSS for 9 days, while the intervention of KOS and sulfasalazine continued.
35506498	7	49	dep	structures	1015:1024	arg1	the					1011:1013	the	1011:1013	the	1011:1013	Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment.
35506498	2	50	theme	200	312:314	arg1	mg					316:317	mg	316:317	mg	316:317	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	8	51	theme	possible	1117:1124	arg1	mechanism					1135:1143	The possible internal mechanism	1113:1143	The possible internal mechanism	1113:1143	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	5	52	theme	p	844:844	arg1	proteins					834:841	tight junction proteins	819:841	tight junction proteins (p < 0.05)	819:852	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	5	52	theme	p	844:844	arg1	<					846:846	p < 0.05	844:851	p < 0.05	844:851	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	6	53	theme	mice	926:929	arg1	tissue					904:909	the colon tissue	894:909	the colon tissue of the colitis mice	894:929	The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal.
35506498	6	54	from	level	859:863	arg1	tissue					904:909	the colon tissue	894:909	the colon tissue of the colitis mice	894:929	The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal.
35506498	8	55	dep	Proteobacteria	1216:1229	arg1	Campilobacterota					1232:1247	Campilobacterota	1232:1247	Campilobacterota	1232:1247	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	8	55	dep	Proteobacteria	1216:1229	arg1	Clostridiaceae					1253:1266	Clostridiaceae	1253:1266	Clostridiaceae	1253:1266	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	1	56	from	disease	200:206	arg1	mice					219:222	colitis mice	211:222	colitis mice	211:222	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	1	57	theme	protective	127:136	arg1	effect					138:143	the protective effect	123:143	the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice	123:222	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	8	58	dep	down-regulates	1157:1170	arg1	Proteobacteria					1216:1229	Proteobacteria	1216:1229	Proteobacteria	1216:1229	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	2	59	theme	kg-1	302:305	arg1	KOS					307:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	200 mg kg-1 or 600 mg kg-1 KOS	280:309	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	7	60	theme	intestinal	1049:1058	arg1	microorganisms					1060:1073	the intestinal microorganisms	1045:1073	the intestinal microorganisms	1045:1073	Moreover, the analysis of the gut microbiota revealed that the structures and composition of the intestinal microorganisms were also regulated by KOS treatment.
35506498	4	61	theme	gut	639:641	arg1	microbiota					643:652	gut microbiota	639:652	gut microbiota	639:652	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	0	62	theme	mechanistic	74:84	arg1	insights					86:93	mechanistic insights	74:93	mechanistic insights	74:93	Konjac oligosaccharides attenuate DSS-induced ulcerative colitis in mice: mechanistic insights.
35506498	5	63	theme	tissue	770:775	arg1	lesion					750:755	alleviated pathological lesion	726:755	alleviated pathological lesion of the colon tissue	726:775	The results showed that mice treated with KOS exhibited alleviated pathological lesion of the colon tissue and significantly increased expression of tight junction proteins (p < 0.05).
35506498	9	64	theme	dietary	1413:1419	arg1	management					1421:1430	dietary management	1413:1430	dietary management for patients with inflammatory bowel disease	1413:1475	These findings provide new insights into dietary management for patients with inflammatory bowel disease.
35506498	4	65	theme	colon	604:608	arg1	phenotype					566:574	phenotype	566:574	phenotype	566:574	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	4	65	theme	colon	604:608	arg1	lesion					590:595	pathological lesion	577:595	pathological lesion of the colon	577:608	At the end of the experiment, the phenotype, pathological lesion of the colon, parameters of cytokines and gut microbiota were evaluated.
35506498	6	66	theme	colitis	918:924	arg1	mice					926:929	the colitis mice	914:929	the colitis mice	914:929	The level of inflammatory cytokines in the colon tissue of the colitis mice tended to be normal.
35506498	8	67	theme	internal	1126:1133	arg1	mechanism					1135:1143	The possible internal mechanism	1113:1143	The possible internal mechanism	1113:1143	The possible internal mechanism is that KOS down-regulates the abundance of pro-inflammatory bacteria (Proteobacteria, Campilobacterota and Clostridiaceae) and up-regulates the abundance of anti-inflammatory bacteria (Bifidobacteriaceae and Akkermansiaceae).
35506498	2	68	theme	experimental	236:247	arg1	period					249:254	the experimental period	232:254	the experimental period	232:254	During the experimental period, mice were administered 200 mg kg-1 or 600 mg kg-1 KOS, 200 mg kg-1 sulfasalazine and a combination of KOS and sulfasalazine for 14 days.
35506498	1	69	theme	konjac	148:153	arg1	KOS					173:175	KOS	173:175	KOS	173:175	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
35506498	1	69	theme	konjac	148:153	arg1	oligosaccharides					155:170	konjac oligosaccharides	148:170	konjac oligosaccharides (KOS)	148:176	This study aims to explore the protective effect of konjac oligosaccharides (KOS) on inflammatory bowel disease in colitis mice.
36175227	9	0	theme	lactose	1345:1351	arg1	contents					1353:1360	lactose contents	1345:1360	lactose contents	1345:1360	Compared with controls, supplemental CLA did not affect milk production, DM intake, or milk protein and lactose contents.
36175227	13	1	theme	diet-induced	2124:2135	arg1	MFD					2137:2139	diet-induced MFD	2124:2139	diet-induced MFD	2124:2139	The results provide evidence for an important role of MFGM proteins in regulating MFG diameter, and they facilitate a mechanistic understanding of diet-induced MFD.
36175227	10	2	theme	g/100	1407:1411	arg1	mL					1413:1414	mL	1413:1414	mL	1413:1414	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	1	3	theme	fat	246:248	arg1	MFG					260:262	MFG	260:262	MFG	260:262	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	1	3	theme	fat	246:248	arg1	globules					250:257	milk fat globules	241:257	milk fat globules (MFG)	241:263	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	10	4	dep	content	1393:1399	arg1	3.73					1402:1405	3.73	1402:1405	3.73	1402:1405	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	4	dep	content	1393:1399	arg1	2.47					1420:1423	2.47	1420:1423	2.47	1420:1423	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	3	5	from	MFD	584:586	arg1	milk					627:630	bovine milk	620:630	bovine milk	620:630	However, little is known about the relationship between MFD, MFG size, and MFGM proteins in bovine milk.
36175227	3	6	theme	bovine	620:625	arg1	milk					627:630	bovine milk	620:630	bovine milk	620:630	However, little is known about the relationship between MFD, MFG size, and MFGM proteins in bovine milk.
36175227	1	7	theme	globule	163:169	arg1	proteins					187:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	6	8	theme	dry	958:960	arg1	DM					970:971	DM	970:971	DM	970:971	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	6	8	theme	dry	958:960	arg1	matter					962:967	dry matter	958:967	dry matter (DM)	958:972	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	10	9	theme	surface	1599:1605	arg1	area					1607:1610	specific surface area	1590:1610	specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg)	1590:1647	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	11	10	dep	downregulated	1789:1801	arg1	117					1785:1787	117	1785:1787	117	1785:1787	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	0	11	theme	conjugated	100:109	arg1	depression					142:151	conjugated linoleic acid-induced milk fat depression	100:151	conjugated linoleic acid-induced milk fat depression	100:151	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	2	12	theme	fat	315:317	arg1	globules					319:326	fat globules	315:326	fat globules	315:326	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	0	13	theme	acid-induced	120:131	arg1	fat					138:140	linoleic acid-induced milk fat	111:140	conjugated linoleic acid-induced milk fat depression	100:151	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	5	14	theme	mid-lactating	738:750	arg1	cows					761:764	Sixteen mid-lactating Holstein cows	730:764	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity	730:824	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	5	14	theme	mid-lactating	738:750	arg1	±					771:771	145 ± 24 d in milk	767:784	145 ± 24 d in milk	767:784	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	2	15	theme	droplet	514:520	arg1	size					522:525	mammary cellular lipid droplet size	491:525	mammary cellular lipid droplet size	491:525	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	11	16	theme	expressed	1679:1687	arg1	proteins					1689:1696	177 differentially expressed proteins	1660:1696	177 differentially expressed proteins	1660:1696	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	0	17	theme	fat	138:140	arg1	depression					142:151	conjugated linoleic acid-induced milk fat depression	100:151	conjugated linoleic acid-induced milk fat depression	100:151	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	2	18	theme	cellular	499:506	arg1	droplet					514:520	mammary cellular lipid droplet	491:520	mammary cellular lipid droplet size	491:525	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	6	19	theme	=	981:981	arg1	n					979:979	n = 8	979:983	n = 8	979:983	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	6	19	theme	=	981:981	arg1	control					934:940	control	934:940	control	934:940	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	5	20	dep	±	771:771	arg1	d					776:776	24 d	773:776	145 ± 24 d in milk	767:784	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	13	21	theme	MFG	2059:2061	arg1	diameter					2063:2070	MFG diameter	2059:2070	MFG diameter	2059:2070	The results provide evidence for an important role of MFGM proteins in regulating MFG diameter, and they facilitate a mechanistic understanding of diet-induced MFD.
36175227	5	22	theme	similar	792:798	arg1	condition					805:813	similar body condition	792:813	similar body condition	792:813	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	2	23	theme	fat	379:381	arg1	MFD					395:397	MFD	395:397	MFD	395:397	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	2	23	theme	fat	379:381	arg1	depression					383:392	conjugated linoleic acid (CLA)-induced milk fat depression	335:392	conjugated linoleic acid (CLA)-induced milk fat depression (MFD)	335:398	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	11	24	located	detected	1703:1710	arg1	total					1653:1657	total	1653:1657	total	1653:1657	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	11	24	located	detected	1703:1710	arg2	proteins					1689:1696	177 differentially expressed proteins	1660:1696	177 differentially expressed proteins	1660:1696	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	11	24	located	detected	1703:1710	arg1	milk					1715:1718	milk	1715:1718	milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated	1715:1801	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	5	25	from	d	776:776	arg1	milk					781:784	milk	781:784	milk	781:784	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	1	26	theme	Milk	154:157	arg1	proteins					187:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	2	27	from	decrease	291:298	arg1	size					307:310	the size	303:310	the size of fat globules	303:326	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	8	28	theme	MFGM	1226:1229	arg1	proteins					1231:1238	the identified MFGM proteins	1211:1238	the identified MFGM proteins	1211:1238	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	12	29	theme	MFG	1837:1839	arg1	size					1841:1844	MFG size	1837:1844	MFG size	1837:1844	Correlation analysis showed that MFG size was negatively correlated with various proteins, including XDH and FABP3, and positively correlated with MFG-E8, RAB19, and APOA1.
36175227	0	30	theme	milk	75:78	arg1	globules					84:91	milk fat globules	75:91	milk fat globules	75:91	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	9	31	theme	supplemental	1265:1276	arg1	CLA					1278:1280	supplemental CLA	1265:1280	supplemental CLA	1265:1280	Compared with controls, supplemental CLA did not affect milk production, DM intake, or milk protein and lactose contents.
36175227	13	32	theme	MFGM	2031:2034	arg1	proteins					2036:2043	MFGM proteins	2031:2043	MFGM proteins	2031:2043	The results provide evidence for an important role of MFGM proteins in regulating MFG diameter, and they facilitate a mechanistic understanding of diet-induced MFD.
36175227	8	33	theme	MFGM	1079:1082	arg1	proteins					1084:1091	MFGM proteins	1079:1091	MFGM proteins	1079:1091	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	0	34	theme	globules	84:91	arg1	size					67:70	the size	63:70	the size of milk fat globules	63:91	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	5	35	theme	control	844:850	arg1	groups					860:865	control and CLA groups	844:865	groups	860:865	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	6	36	dep	diet	909:912	arg1	=					926:926	=	926:926	=	926:926	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	0	37	theme	Milk	0:3	arg1	fat					5:7	Milk fat	0:7	Milk fat globule membrane proteins	0:33	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	6	38	theme	basal	903:907	arg1	diet					909:912	a basal diet	901:912	a basal diet (control, n = 8)	901:929	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	4	39	theme	study	649:653	arg1	aim					637:639	The aim	633:639	The aim of this study	633:653	The aim of this study was to investigate the profile of MFGM proteins during MFD induced by CLA.
36175227	10	40	theme	milk	1384:1387	arg1	content					1393:1399	milk fat content	1384:1399	milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL)	1384:1433	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	0	41	theme	globule	9:15	arg1	proteins					26:33	Milk fat globule membrane proteins	0:33	Milk fat globule membrane proteins	0:33	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	2	42	theme	-induced	365:372	arg1	MFD					395:397	MFD	395:397	MFD	395:397	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	2	42	theme	-induced	365:372	arg1	depression					383:392	conjugated linoleic acid (CLA)-induced milk fat depression	335:392	conjugated linoleic acid (CLA)-induced milk fat depression (MFD)	335:398	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	1	43	theme	fat	159:161	arg1	proteins					187:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	5	44	theme	10-d	874:877	arg1	period					879:884	a 10-d period	872:884	a 10-d period	872:884	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	7	45	theme	MFG	1040:1042	arg1	size					1044:1047	MFG size	1040:1047	MFG size	1040:1047	Cow performance, milk composition, and MFG size were measured daily.
36175227	8	46	theme	proteomic	1139:1147	arg1	analysis					1149:1156	quantitative proteomic analysis	1126:1156	quantitative proteomic analysis	1126:1156	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	9	47	theme	DM	1314:1315	arg1	intake					1317:1322	DM intake	1314:1322	DM intake	1314:1322	Compared with controls, supplemental CLA did not affect milk production, DM intake, or milk protein and lactose contents.
36175227	9	48	theme	milk	1328:1331	arg1	protein					1333:1339	milk protein	1328:1339	milk protein	1328:1339	Compared with controls, supplemental CLA did not affect milk production, DM intake, or milk protein and lactose contents.
36175227	7	49	theme	milk	1018:1021	arg1	composition					1023:1033	milk composition	1018:1033	milk composition	1018:1033	Cow performance, milk composition, and MFG size were measured daily.
36175227	3	50	theme	MFG	589:591	arg1	size					593:596	MFG size	589:596	MFG size	589:596	However, little is known about the relationship between MFD, MFG size, and MFGM proteins in bovine milk.
36175227	8	51	theme	western	1163:1169	arg1	blotting					1171:1178	western blotting	1163:1178	western blotting	1163:1178	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	2	52	theme	conjugated	335:344	arg1	CLA					361:363	CLA	361:363	CLA	361:363	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	2	52	theme	conjugated	335:344	arg1	acid					355:358	conjugated linoleic acid	335:358	conjugated linoleic acid (CLA)	335:364	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	3	53	from	size	593:596	arg1	milk					627:630	bovine milk	620:630	bovine milk	620:630	However, little is known about the relationship between MFD, MFG size, and MFGM proteins in bovine milk.
36175227	2	54	theme	globules	319:326	arg1	size					307:310	the size	303:310	the size of fat globules	303:326	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	1	55	theme	milk	241:244	arg1	MFG					260:262	MFG	260:262	MFG	260:262	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	1	55	theme	milk	241:244	arg1	globules					250:257	milk fat globules	241:257	milk fat globules (MFG)	241:263	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	11	56	theme	cows	1725:1728	arg1	60					1752:1753	60	1752:1753	60	1752:1753	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	11	56	theme	cows	1725:1728	arg1	cows					1725:1728	cows	1725:1728	cows	1725:1728	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	11	57	with	cows	1725:1728	arg1	MFD					1747:1749	CLA-induced MFD	1735:1749	CLA-induced MFD	1735:1749	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	3	58	theme	MFGM	603:606	arg1	proteins					608:615	MFGM proteins	603:615	MFGM proteins	603:615	However, little is known about the relationship between MFD, MFG size, and MFGM proteins in bovine milk.
36175227	13	59	theme	MFD	2137:2139	arg1	understanding					2107:2119	a mechanistic understanding	2093:2119	a mechanistic understanding of diet-induced MFD	2093:2139	The results provide evidence for an important role of MFGM proteins in regulating MFG diameter, and they facilitate a mechanistic understanding of diet-induced MFD.
36175227	5	60	theme	Holstein	752:759	arg1	cows					761:764	Sixteen mid-lactating Holstein cows	730:764	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity	730:824	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	5	60	theme	Holstein	752:759	arg1	±					771:771	145 ± 24 d in milk	767:784	145 ± 24 d in milk	767:784	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	6	61	theme	matter	962:967	arg1	n					979:979	n = 8	979:983	n = 8	979:983	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	6	61	theme	matter	962:967	arg1	diet					909:912	a basal diet	901:912	a basal diet (control, n = 8)	901:929	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	6	61	theme	matter	962:967	arg1	control					934:940	control	934:940	control	934:940	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	6	61	theme	matter	962:967	arg1	g/kg					950:953	15 g/kg	947:953	15 g/kg of dry matter (DM)	947:972	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	13	62	theme	mechanistic	2095:2105	arg1	understanding					2107:2119	a mechanistic understanding	2093:2119	a mechanistic understanding of diet-induced MFD	2093:2139	The results provide evidence for an important role of MFGM proteins in regulating MFG diameter, and they facilitate a mechanistic understanding of diet-induced MFD.
36175227	8	63	used	used	1184:1187	arg2	blotting					1171:1178	western blotting	1163:1178	western blotting	1163:1178	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	10	64	theme	volume-related	1459:1472	arg1	diameter					1474:1481	volume-related diameter D[4,3]	1459:1488	volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm)	1459:1510	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	64	theme	volume-related	1459:1472	arg1	parameters					1448:1457	the size parameters	1439:1457	the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1439:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	1	65	theme	membrane	171:178	arg1	proteins					187:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	11	66	theme	CLA-induced	1735:1745	arg1	MFD					1747:1749	CLA-induced MFD	1735:1749	CLA-induced MFD	1735:1749	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	10	67	theme	specific	1590:1597	arg1	area					1607:1610	specific surface area	1590:1610	specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg)	1590:1647	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	0	68	theme	linoleic	111:118	arg1	fat					138:140	linoleic acid-induced milk fat	111:140	conjugated linoleic acid-induced milk fat depression	100:151	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	6	69	dep	=	926:926	arg1	control					915:921	control	915:921	control	915:921	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	10	70	theme	size	1443:1446	arg1	D[3,2					1546:1550	surface area-related diameter D[3,2]	1516:1551	surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1516:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	70	theme	size	1443:1446	arg1	diameter					1474:1481	volume-related diameter D[4,3]	1459:1488	volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm)	1459:1510	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	70	theme	size	1443:1446	arg1	parameters					1448:1457	the size parameters	1439:1457	the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1439:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	1	71	theme	MFGM	181:184	arg1	proteins					187:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins	154:194	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	10	72	dep	diameter	1474:1481	arg1	μm					1496:1497	3.72 μm vs. 3.35 μm	1491:1509	μm	1496:1497	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	72	dep	diameter	1474:1481	arg1	μm					1508:1509	3.72 μm vs. 3.35 μm	1491:1509	μm	1508:1509	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	0	73	theme	milk	133:136	arg1	fat					138:140	linoleic acid-induced milk fat	111:140	conjugated linoleic acid-induced milk fat depression	100:151	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	2	74	theme	central	464:470	arg1	role					472:475	a central role	462:475	a central role	462:475	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	3	75	from	proteins	608:615	arg1	milk					627:630	bovine milk	620:630	bovine milk	620:630	However, little is known about the relationship between MFD, MFG size, and MFGM proteins in bovine milk.
36175227	10	76	theme	MFG	1615:1617	arg1	area					1607:1610	specific surface area	1590:1610	specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg)	1590:1647	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	13	77	theme	proteins	2036:2043	arg1	role					2023:2026	an important role	2010:2026	an important role of MFGM proteins in regulating MFG diameter	2010:2070	The results provide evidence for an important role of MFGM proteins in regulating MFG diameter, and they facilitate a mechanistic understanding of diet-induced MFD.
36175227	10	78	dep	MFG	1615:1617	arg1	m2/kg					1626:1630	1,905 m2/kg vs. 2,188 m2/kg	1620:1646	m2/kg	1626:1630	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	78	dep	MFG	1615:1617	arg1	m2/kg					1642:1646	1,905 m2/kg vs. 2,188 m2/kg	1620:1646	m2/kg	1642:1646	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	2	79	theme	lipid	508:512	arg1	droplet					514:520	mammary cellular lipid droplet	491:520	mammary cellular lipid droplet size	491:525	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	11	80	from	cows	1725:1728	arg1	milk					1715:1718	milk	1715:1718	milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated	1715:1801	In total, 177 differentially expressed proteins were detected in milk from cows with CLA-induced MFD, 60 of which were upregulated and 117 downregulated.
36175227	2	81	theme	mammary	491:497	arg1	droplet					514:520	mammary cellular lipid droplet	491:520	mammary cellular lipid droplet size	491:525	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	10	82	dep	parameters	1448:1457	arg1	μm					1571:1572	3.13 μm vs. 2.80 μm	1554:1572	3.13 μm vs. 2.80 μm	1554:1572	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	82	dep	parameters	1448:1457	arg1	diameter					1474:1481	volume-related diameter D[4,3]	1459:1488	volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm)	1459:1510	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	82	dep	parameters	1448:1457	arg1	D[3,2					1546:1550	surface area-related diameter D[3,2]	1516:1551	surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1516:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	82	dep	parameters	1448:1457	arg1	parameters					1448:1457	the size parameters	1439:1457	the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1439:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	8	83	theme	identified	1215:1224	arg1	proteins					1231:1238	the identified MFGM proteins	1211:1238	the identified MFGM proteins	1211:1238	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	8	84	theme	proteins	1231:1238	arg1	subset					1201:1206	a subset	1199:1206	a subset of the identified MFGM proteins	1199:1238	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	0	85	theme	fat	80:82	arg1	globules					84:91	milk fat globules	75:91	milk fat globules	75:91	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	5	86	theme	CLA	856:858	arg1	groups					860:865	control and CLA groups	844:865	groups	860:865	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	13	87	theme	important	2013:2021	arg1	role					2023:2026	an important role	2010:2026	an important role of MFGM proteins in regulating MFG diameter	2010:2070	The results provide evidence for an important role of MFGM proteins in regulating MFG diameter, and they facilitate a mechanistic understanding of diet-induced MFD.
36175227	10	88	dep	3.73	1402:1405	arg1	mL					1431:1432	g/100 mL	1425:1432	g/100 mL	1425:1432	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	2	89	theme	MFGM	443:446	arg1	proteins					448:455	some MFGM proteins	438:455	some MFGM proteins	438:455	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	12	90	theme	various	1877:1883	arg1	FABP3					1913:1917	FABP3	1913:1917	FABP3	1913:1917	Correlation analysis showed that MFG size was negatively correlated with various proteins, including XDH and FABP3, and positively correlated with MFG-E8, RAB19, and APOA1.
36175227	12	90	theme	various	1877:1883	arg1	proteins					1885:1892	various proteins	1877:1892	various proteins	1877:1892	Correlation analysis showed that MFG size was negatively correlated with various proteins, including XDH and FABP3, and positively correlated with MFG-E8, RAB19, and APOA1.
36175227	12	90	theme	various	1877:1883	arg1	XDH					1905:1907	XDH	1905:1907	XDH	1905:1907	Correlation analysis showed that MFG size was negatively correlated with various proteins, including XDH and FABP3, and positively correlated with MFG-E8, RAB19, and APOA1.
36175227	0	91	theme	fat	5:7	arg1	proteins					26:33	Milk fat globule membrane proteins	0:33	Milk fat globule membrane proteins	0:33	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	5	92	theme	body	800:803	arg1	condition					805:813	similar body condition	792:813	similar body condition	792:813	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	10	93	theme	area-related	1524:1535	arg1	μm					1571:1572	3.13 μm vs. 2.80 μm	1554:1572	3.13 μm vs. 2.80 μm	1554:1572	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	93	theme	area-related	1524:1535	arg1	D[3,2					1546:1550	surface area-related diameter D[3,2]	1516:1551	surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1516:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	93	theme	area-related	1524:1535	arg1	parameters					1448:1457	the size parameters	1439:1457	the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1439:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	2	94	theme	linoleic	346:353	arg1	CLA					361:363	CLA	361:363	CLA	361:363	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	2	94	theme	linoleic	346:353	arg1	acid					355:358	conjugated linoleic acid	335:358	conjugated linoleic acid (CLA)	335:364	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	0	95	theme	membrane	17:24	arg1	proteins					26:33	Milk fat globule membrane proteins	0:33	Milk fat globule membrane proteins	0:33	Milk fat globule membrane proteins are involved in controlling the size of milk fat globules during conjugated linoleic acid-induced milk fat depression.
36175227	4	96	theme	proteins	694:701	arg1	profile					678:684	the profile	674:684	the profile of MFGM proteins	674:701	The aim of this study was to investigate the profile of MFGM proteins during MFD induced by CLA.
36175227	9	97	theme	milk	1297:1300	arg1	production					1302:1311	milk production	1297:1311	milk production	1297:1311	Compared with controls, supplemental CLA did not affect milk production, DM intake, or milk protein and lactose contents.
36175227	10	98	theme	surface	1516:1522	arg1	μm					1571:1572	3.13 μm vs. 2.80 μm	1554:1572	3.13 μm vs. 2.80 μm	1554:1572	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	98	theme	surface	1516:1522	arg1	D[3,2					1546:1550	surface area-related diameter D[3,2]	1516:1551	surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1516:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	98	theme	surface	1516:1522	arg1	parameters					1448:1457	the size parameters	1439:1457	the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1439:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	2	99	theme	milk	374:377	arg1	fat					379:381	milk fat	374:381	conjugated linoleic acid (CLA)-induced milk fat depression (MFD)	335:398	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	2	100	theme	other	405:409	arg1	studies					411:417	other studies	405:417	other studies	405:417	We previously detected a decrease in the size of fat globules during conjugated linoleic acid (CLA)-induced milk fat depression (MFD), and other studies have reported that some MFGM proteins play a central role in regulating mammary cellular lipid droplet size.
36175227	8	101	theme	quantitative	1126:1137	arg1	analysis					1149:1156	quantitative proteomic analysis	1126:1156	quantitative proteomic analysis	1126:1156	On d 10, MFGM proteins were extracted and identified by quantitative proteomic analysis, and western blotting was used to verify a subset of the identified MFGM proteins.
36175227	4	102	theme	MFGM	689:692	arg1	proteins					694:701	MFGM proteins	689:701	MFGM proteins	689:701	The aim of this study was to investigate the profile of MFGM proteins during MFD induced by CLA.
36175227	5	103	with	cows	761:764	arg1	parity					819:824	parity	819:824	parity	819:824	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	5	103	with	cows	761:764	arg1	condition					805:813	similar body condition	792:813	similar body condition	792:813	Sixteen mid-lactating Holstein cows (145 ± 24 d in milk) with similar body condition and parity were divided into control and CLA groups over a 10-d period.
36175227	10	104	theme	diameter	1537:1544	arg1	μm					1571:1572	3.13 μm vs. 2.80 μm	1554:1572	3.13 μm vs. 2.80 μm	1554:1572	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	104	theme	diameter	1537:1544	arg1	D[3,2					1546:1550	surface area-related diameter D[3,2]	1516:1551	surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1516:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	104	theme	diameter	1537:1544	arg1	parameters					1448:1457	the size parameters	1439:1457	the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm)	1439:1573	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	10	105	theme	fat	1389:1391	arg1	content					1393:1399	milk fat content	1384:1399	milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL)	1384:1433	However, CLA reduced milk fat content (3.73 g/100 mL vs. 2.47 g/100 mL) and the size parameters volume-related diameter D[4,3] (3.72 μm vs. 3.35 μm) and surface area-related diameter D[3,2] (3.13 μm vs. 2.80 μm), but increased specific surface area of MFG (1,905 m2/kg vs. 2,188 m2/kg).
36175227	1	106	theme	triacylglycerol	209:223	arg1	core					225:228	the triacylglycerol core	205:228	the triacylglycerol core comprising milk fat globules (MFG)	205:263	Milk fat globule membrane (MFGM) proteins surround the triacylglycerol core comprising milk fat globules (MFG).
36175227	7	107	theme	Cow	1001:1003	arg1	performance					1005:1015	Cow performance	1001:1015	Cow performance	1001:1015	Cow performance, milk composition, and MFG size were measured daily.
36175227	6	108	dep	control	934:940	arg1	CLA					974:976	CLA	974:976	CLA	974:976	Cows were fed a basal diet (control, n = 8) or control plus 15 g/kg of dry matter (DM) CLA (n = 8) to induce MFD.
36175227	12	109	theme	Correlation	1804:1814	arg1	analysis					1816:1823	Correlation analysis	1804:1823	Correlation analysis	1804:1823	Correlation analysis showed that MFG size was negatively correlated with various proteins, including XDH and FABP3, and positively correlated with MFG-E8, RAB19, and APOA1.
37061125	1	0	theme	gut	269:271	arg1	dysbiosis					284:292	gut microbiota dysbiosis	269:292	gut microbiota dysbiosis	269:292	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	7	1	from	potent	1283:1288	arg1	model					1312:1316	the DSS-induced UC model	1293:1316	the DSS-induced UC model	1293:1316	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	6	2	theme	probiotics	1206:1215	arg1	treatment					1217:1225	probiotics treatment	1206:1225	probiotics treatment	1206:1225	Moreover, the composition and function of microbiota were altered in IBD zebrafish, and probiotics treatment displayed prominent microbiota features.
37061125	3	3	theme	mucosal	544:550	arg1	function					560:567	intestinal mucosal barrier function	533:567	intestinal mucosal barrier function	533:567	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	6	4	theme	IBD	1187:1189	arg1	zebrafish					1191:1199	IBD zebrafish	1187:1199	IBD zebrafish	1187:1199	Moreover, the composition and function of microbiota were altered in IBD zebrafish, and probiotics treatment displayed prominent microbiota features.
37061125	3	5	theme	IBD	587:589	arg1	zebrafish					591:599	IBD zebrafish	587:599	IBD zebrafish	587:599	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	5	6	theme	mucosal	928:934	arg1	function					944:951	intestinal mucosal barrier function	917:951	intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC)	917:1020	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	10	7	theme	probiotics	1622:1631	arg1	impact					1612:1617	The impact	1608:1617	The impact of probiotics on IBD	1608:1638	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	0	8	theme	mucosal	129:135	arg1	barrier					137:143	the intestinal mucosal barrier	114:143	the intestinal mucosal barrier	114:143	Bifidobacterium and Lactobacillus improve inflammatory bowel disease in zebrafish of different ages by regulating the intestinal mucosal barrier and microbiota.
37061125	2	9	theme	gut	320:322	arg1	microbiota					324:333	the gut microbiota	316:333	the gut microbiota	316:333	Strategies targeting the gut microbiota are potential therapies for preventing and ameliorating IBD.
37061125	5	10	theme	inflammatory	877:888	arg1	markers					890:896	inflammatory markers	877:896	inflammatory markers	877:896	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	8	11	theme	Enhydrobacter	1478:1490	arg1	abundance					1465:1473	the abundance	1461:1473	the abundance of Enhydrobacter and acetic acid levels	1461:1513	LC122 exerted better protection against TNBS-induced CD, and increased the abundance of Enhydrobacter and acetic acid levels.
37061125	4	12	theme	LC122	661:665	arg1	treatment					667:675	KEY FINDINGS BL986 and LC122 treatment	638:675	KEY FINDINGS BL986 and LC122 treatment	638:675	KEY FINDINGS BL986 and LC122 treatment promoted the development and increased the microbiota diversity in larval zebrafish.
37061125	3	13	theme	ages	614:617	arg1	microbiota					573:582	microbiota	573:582	microbiota	573:582	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	3	13	theme	ages	614:617	arg1	function					560:567	intestinal mucosal barrier function	533:567	intestinal mucosal barrier function	533:567	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	8	14	theme	acetic	1496:1501	arg1	acid					1503:1506	acetic acid	1496:1506	acetic acid	1496:1506	LC122 exerted better protection against TNBS-induced CD, and increased the abundance of Enhydrobacter and acetic acid levels.
37061125	5	15	theme	disease	1084:1090	arg1	models					1097:1102	2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models	1026:1102	2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models	1026:1102	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	10	16	theme	practical	1799:1807	arg1	usage					1809:1813	the practical usage	1795:1813	the practical usage of probiotics	1795:1827	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	6	17	theme	microbiota	1247:1256	arg1	features					1258:1265	prominent microbiota features	1237:1265	prominent microbiota features	1237:1265	Moreover, the composition and function of microbiota were altered in IBD zebrafish, and probiotics treatment displayed prominent microbiota features.
37061125	3	18	from	microbiota	573:582	arg1	zebrafish					591:599	IBD zebrafish	587:599	IBD zebrafish	587:599	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	5	19	theme	CD	1093:1094	arg1	models					1097:1102	2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models	1026:1102	2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models	1026:1102	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	9	20	theme	probiotics	1543:1552	arg1	stronger					1558:1565	stronger	1558:1565	stronger	1558:1565	Furthermore, the effect of probiotics was stronger in larval and aged zebrafish.
37061125	9	20	theme	probiotics	1543:1552	arg1	effect					1533:1538	the effect	1529:1538	the effect of probiotics	1529:1552	Furthermore, the effect of probiotics was stronger in larval and aged zebrafish.
37061125	7	21	theme	acid	1377:1380	arg1	abundance					1337:1345	the abundance	1333:1345	the abundance of Faecalibaculum and butyric acid levels	1333:1387	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	3	22	dep	METHODS	401:407	arg1	investigated					624:635	investigated	624:635	were investigated	619:635	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	4	23	theme	microbiota	720:729	arg1	diversity					731:739	the microbiota diversity	716:739	the microbiota diversity in larval zebrafish	716:759	KEY FINDINGS BL986 and LC122 treatment promoted the development and increased the microbiota diversity in larval zebrafish.
37061125	0	24	theme	different	85:93	arg1	ages					95:98	different ages	85:98	different ages	85:98	Bifidobacterium and Lactobacillus improve inflammatory bowel disease in zebrafish of different ages by regulating the intestinal mucosal barrier and microbiota.
37061125	1	25	theme	bowel	179:183	arg1	IBD					194:196	IBD	194:196	IBD	194:196	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	1	25	theme	bowel	179:183	arg1	disease					185:191	Inflammatory bowel disease	166:191	Inflammatory bowel disease (IBD) patients	166:206	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	3	26	from	function	560:567	arg1	zebrafish					591:599	IBD zebrafish	587:599	IBD zebrafish	587:599	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	9	27	theme	aged	1581:1584	arg1	zebrafish					1586:1594	larval and aged zebrafish	1570:1594	larval and aged zebrafish	1570:1594	Furthermore, the effect of probiotics was stronger in larval and aged zebrafish.
37061125	0	28	from	disease	61:67	arg1	zebrafish					72:80	zebrafish	72:80	zebrafish of different ages	72:98	Bifidobacterium and Lactobacillus improve inflammatory bowel disease in zebrafish of different ages by regulating the intestinal mucosal barrier and microbiota.
37061125	0	29	theme	bowel	55:59	arg1	disease					61:67	inflammatory bowel disease	42:67	inflammatory bowel disease in zebrafish of different ages	42:98	Bifidobacterium and Lactobacillus improve inflammatory bowel disease in zebrafish of different ages by regulating the intestinal mucosal barrier and microbiota.
37061125	7	30	theme	butyric	1369:1375	arg1	acid					1377:1380	butyric acid	1369:1380	butyric acid	1369:1380	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	3	31	theme	potential	413:421	arg1	roles					423:427	The potential roles	409:427	The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages	409:617	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	8	32	theme	better	1404:1409	arg1	protection					1411:1420	better protection	1404:1420	better protection against TNBS-induced CD	1404:1444	LC122 exerted better protection against TNBS-induced CD, and increased the abundance of Enhydrobacter and acetic acid levels.
37061125	10	33	theme	IBD	1674:1676	arg1	age					1686:1688	the age	1682:1688	the age of the zebrafish	1682:1705	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	10	33	theme	IBD	1674:1676	arg1	subtypes					1662:1669	the subtypes	1658:1669	the subtypes of IBD	1658:1676	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	7	34	theme	Faecalibaculum	1350:1363	arg1	abundance					1337:1345	the abundance	1333:1345	the abundance of Faecalibaculum and butyric acid levels	1333:1387	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	10	35	theme	zebrafish	1697:1705	arg1	age					1686:1688	the age	1682:1688	the age of the zebrafish	1682:1705	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	10	35	theme	zebrafish	1697:1705	arg1	subtypes					1662:1669	the subtypes	1658:1669	the subtypes of IBD	1658:1676	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	5	36	theme	ulcerative	998:1007	arg1	UC					1018:1019	UC	1018:1019	UC	1018:1019	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	5	36	theme	ulcerative	998:1007	arg1	colitis					1009:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis	956:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC)	956:1020	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	3	37	theme	probiotic	436:444	arg1	longum					470:475	Bifidobacterium longum	454:475	Bifidobacterium longum BL986 (BL986)	454:489	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	3	37	theme	probiotic	436:444	arg1	casei					509:513	Lactobacillus casei	495:513	Lactobacillus casei LC122 (LC122)	495:527	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	3	37	theme	probiotic	436:444	arg1	stains					446:451	two probiotic stains	432:451	two probiotic stains	432:451	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	0	38	theme	inflammatory	42:53	arg1	disease					61:67	inflammatory bowel disease	42:67	inflammatory bowel disease in zebrafish of different ages	42:98	Bifidobacterium and Lactobacillus improve inflammatory bowel disease in zebrafish of different ages by regulating the intestinal mucosal barrier and microbiota.
37061125	5	39	theme	mucus	830:834	arg1	secretion					836:844	intestinal mucus secretion	819:844	intestinal mucus secretion	819:844	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	5	40	theme	-induced	1067:1074	arg1	models					1097:1102	2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models	1026:1102	2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models	1026:1102	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	6	41	dep	composition	1132:1142	arg1	the					1128:1130	the	1128:1130	the	1128:1130	Moreover, the composition and function of microbiota were altered in IBD zebrafish, and probiotics treatment displayed prominent microbiota features.
37061125	5	42	dep	markers	890:896	arg1	improving					907:915	improving	907:915	improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish	907:1115	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	7	43	dep	Faecalibaculum	1350:1363	arg1	levels					1382:1387	levels	1382:1387	levels	1382:1387	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	1	44	theme	impaired	227:234	arg1	integrity					255:263	impaired intestinal barrier integrity	227:263	impaired intestinal barrier integrity	227:263	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	7	45	theme	UC	1309:1310	arg1	model					1312:1316	the DSS-induced UC model	1293:1316	the DSS-induced UC model	1293:1316	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	10	46	theme	age	1744:1746	arg1	types					1723:1727	the types	1719:1727	the types of disease and age	1719:1746	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	4	47	from	diversity	731:739	arg1	zebrafish					751:759	larval zebrafish	744:759	larval zebrafish	744:759	KEY FINDINGS BL986 and LC122 treatment promoted the development and increased the microbiota diversity in larval zebrafish.
37061125	1	48	theme	barrier	247:253	arg1	integrity					255:263	impaired intestinal barrier integrity	227:263	impaired intestinal barrier integrity	227:263	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	10	49	theme	disease	1732:1738	arg1	types					1723:1727	the types	1719:1727	the types of disease and age	1719:1746	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	3	50	theme	barrier	552:558	arg1	function					560:567	intestinal mucosal barrier function	533:567	intestinal mucosal barrier function	533:567	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	1	51	theme	microbiota	273:282	arg1	dysbiosis					284:292	gut microbiota dysbiosis	269:292	gut microbiota dysbiosis	269:292	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	3	52	theme	intestinal	533:542	arg1	function					560:567	intestinal mucosal barrier function	533:567	intestinal mucosal barrier function	533:567	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	1	53	theme	disease	185:191	arg1	patients					199:206	Inflammatory bowel disease (IBD) patients	166:206	Inflammatory bowel disease (IBD) patients	166:206	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	2	54	theme	potential	339:347	arg1	therapies					349:357	potential therapies	339:357	potential therapies for preventing and ameliorating IBD	339:393	Strategies targeting the gut microbiota are potential therapies for preventing and ameliorating IBD.
37061125	2	54	theme	potential	339:347	arg1	Strategies					295:304	Strategies	295:304	Strategies targeting the gut microbiota	295:333	Strategies targeting the gut microbiota are potential therapies for preventing and ameliorating IBD.
37061125	5	55	theme	intestinal	917:926	arg1	function					944:951	intestinal mucosal barrier function	917:951	intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC)	917:1020	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	7	56	from	model	1312:1316	arg1	potent					1283:1288	potent	1283:1288	potent	1283:1288	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	9	57	theme	larval	1570:1575	arg1	zebrafish					1586:1594	larval and aged zebrafish	1570:1594	larval and aged zebrafish	1570:1594	Furthermore, the effect of probiotics was stronger in larval and aged zebrafish.
37061125	4	58	theme	FINDINGS	642:649	arg1	treatment					667:675	KEY FINDINGS BL986 and LC122 treatment	638:675	KEY FINDINGS BL986 and LC122 treatment	638:675	KEY FINDINGS BL986 and LC122 treatment promoted the development and increased the microbiota diversity in larval zebrafish.
37061125	3	59	theme	different	604:612	arg1	ages					614:617	different ages	604:617	different ages	604:617	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	5	60	theme	markers	890:896	arg1	expression					863:872	the expression	859:872	the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish	859:1115	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	8	61	theme	acid	1503:1506	arg1	abundance					1465:1473	the abundance	1461:1473	the abundance of Enhydrobacter and acetic acid levels	1461:1513	LC122 exerted better protection against TNBS-induced CD, and increased the abundance of Enhydrobacter and acetic acid levels.
37061125	6	62	theme	prominent	1237:1245	arg1	features					1258:1265	prominent microbiota features	1237:1265	prominent microbiota features	1237:1265	Moreover, the composition and function of microbiota were altered in IBD zebrafish, and probiotics treatment displayed prominent microbiota features.
37061125	4	63	theme	larval	744:749	arg1	zebrafish					751:759	larval zebrafish	744:759	larval zebrafish	744:759	KEY FINDINGS BL986 and LC122 treatment promoted the development and increased the microbiota diversity in larval zebrafish.
37061125	5	64	theme	barrier	936:942	arg1	function					944:951	intestinal mucosal barrier function	917:951	intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC)	917:1020	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	0	65	theme	ages	95:98	arg1	zebrafish					72:80	zebrafish	72:80	zebrafish of different ages	72:98	Bifidobacterium and Lactobacillus improve inflammatory bowel disease in zebrafish of different ages by regulating the intestinal mucosal barrier and microbiota.
37061125	1	66	theme	Inflammatory	166:177	arg1	IBD					194:196	IBD	194:196	IBD	194:196	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	1	66	theme	Inflammatory	166:177	arg1	disease					185:191	Inflammatory bowel disease	166:191	Inflammatory bowel disease (IBD) patients	166:206	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	10	67	dep	CONCLUSION	1597:1606	arg1	differ					1646:1651	differ	1646:1651	differ	1646:1651	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	10	68	theme	full	1769:1772	arg1	consideration					1774:1786	full consideration	1769:1786	full consideration	1769:1786	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	3	69	theme	MAIN	396:399	arg1	METHODS					401:407	MAIN METHODS	396:407	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.	396:636	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	3	70	dep	casei	509:513	arg1	LC122					515:519	LC122	515:519	LC122	515:519	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	3	70	dep	casei	509:513	arg1	LC122					522:526	LC122	522:526	LC122	522:526	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	5	71	from	models	1097:1102	arg1	UC					1018:1019	UC	1018:1019	UC	1018:1019	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	5	71	from	models	1097:1102	arg1	colitis					1009:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis	956:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC)	956:1020	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	5	72	from	function	944:951	arg1	UC					1018:1019	UC	1018:1019	UC	1018:1019	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	5	72	from	function	944:951	arg1	colitis					1009:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis	956:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC)	956:1020	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	0	73	theme	intestinal	118:127	arg1	barrier					137:143	the intestinal mucosal barrier	114:143	the intestinal mucosal barrier	114:143	Bifidobacterium and Lactobacillus improve inflammatory bowel disease in zebrafish of different ages by regulating the intestinal mucosal barrier and microbiota.
37061125	3	74	from	roles	423:427	arg1	microbiota					573:582	microbiota	573:582	microbiota	573:582	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	3	74	from	roles	423:427	arg1	function					560:567	intestinal mucosal barrier function	533:567	intestinal mucosal barrier function	533:567	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	5	75	theme	-induced	989:996	arg1	UC					1018:1019	UC	1018:1019	UC	1018:1019	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	5	75	theme	-induced	989:996	arg1	colitis					1009:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis	956:1015	dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC)	956:1020	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	10	76	theme	probiotics	1818:1827	arg1	usage					1809:1813	the practical usage	1795:1813	the practical usage of probiotics	1795:1827	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	1	77	dep	AIMS	161:164	arg1	accompanied					212:222	accompanied	212:222	are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis	208:292	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	5	78	theme	intestinal	819:828	arg1	secretion					836:844	intestinal mucus secretion	819:844	intestinal mucus secretion	819:844	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	3	79	theme	stains	446:451	arg1	roles					423:427	The potential roles	409:427	The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages	409:617	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	8	80	theme	TNBS-induced	1430:1441	arg1	CD					1443:1444	TNBS-induced CD	1430:1444	TNBS-induced CD	1430:1444	LC122 exerted better protection against TNBS-induced CD, and increased the abundance of Enhydrobacter and acetic acid levels.
37061125	3	81	dep	longum	470:475	arg1	BL986					484:488	BL986	484:488	BL986	484:488	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	3	81	dep	longum	470:475	arg1	BL986					477:481	BL986	477:481	BL986	477:481	MAIN METHODS The potential roles of two probiotic stains, Bifidobacterium longum BL986 (BL986) and Lactobacillus casei LC122 (LC122), on intestinal mucosal barrier function and microbiota in IBD zebrafish of different ages were investigated.
37061125	6	82	theme	microbiota	1160:1169	arg1	function					1148:1155	function	1148:1155	function	1148:1155	Moreover, the composition and function of microbiota were altered in IBD zebrafish, and probiotics treatment displayed prominent microbiota features.
37061125	6	82	theme	microbiota	1160:1169	arg1	composition					1132:1142	composition	1132:1142	composition	1132:1142	Moreover, the composition and function of microbiota were altered in IBD zebrafish, and probiotics treatment displayed prominent microbiota features.
37061125	8	83	dep	Enhydrobacter	1478:1490	arg1	levels					1508:1513	levels	1508:1513	levels	1508:1513	LC122 exerted better protection against TNBS-induced CD, and increased the abundance of Enhydrobacter and acetic acid levels.
37061125	7	84	theme	DSS-induced	1297:1307	arg1	model					1312:1316	the DSS-induced UC model	1293:1316	the DSS-induced UC model	1293:1316	BL986 was more potent in the DSS-induced UC model, and increased the abundance of Faecalibaculum and butyric acid levels.
37061125	5	85	theme	probiotic	767:775	arg1	treatment					777:785	Both probiotic treatment	762:785	Both probiotic treatment	762:785	Both probiotic treatment ameliorated mortality, promoted intestinal mucus secretion, and reduced the expression of inflammatory markers, thereby improving intestinal mucosal barrier function in dextran sulfate sodium salt (DSS)-induced ulcerative colitis (UC) and 2,4,6-trinitro-benzenesulfonicacid (TNBS)-induced Crohn's disease (CD) models in zebrafish.
37061125	1	86	theme	intestinal	236:245	arg1	integrity					255:263	impaired intestinal barrier integrity	227:263	impaired intestinal barrier integrity	227:263	AIMS Inflammatory bowel disease (IBD) patients are accompanied by impaired intestinal barrier integrity and gut microbiota dysbiosis.
37061125	10	87	from	impact	1612:1617	arg1	IBD					1636:1638	IBD	1636:1638	IBD	1636:1638	CONCLUSION The impact of probiotics on IBD might differ from the subtypes of IBD and the age of the zebrafish, suggesting the types of disease and age should be taken into full consideration during the practical usage of probiotics.
37061125	4	88	theme	KEY	638:640	arg1	treatment					667:675	KEY FINDINGS BL986 and LC122 treatment	638:675	KEY FINDINGS BL986 and LC122 treatment	638:675	KEY FINDINGS BL986 and LC122 treatment promoted the development and increased the microbiota diversity in larval zebrafish.
37047193	7	0	theme	inflammation	1375:1386	arg1	co-cultures					1272:1282	Transwell co-cultures	1262:1282	Transwell co-cultures of Caco-2 cells and U937-derived macrophages	1262:1327	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	0	theme	inflammation	1375:1386	arg1	models					1342:1347	models	1342:1347	models of LPS-induced intestinal inflammation	1342:1386	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	3	1	theme	gut	621:623	arg1	microbiota					625:634	the gut microbiota	617:634	the gut microbiota	617:634	Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity.
37047193	9	2	theme	pro-inflammatory	1819:1834	arg1	cytokines					1836:1844	pro-inflammatory cytokines	1819:1844	pro-inflammatory cytokines	1819:1844	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	7	3	theme	U937-derived	1304:1315	arg1	macrophages					1317:1327	U937-derived macrophages	1304:1327	U937-derived macrophages	1304:1327	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	1	4	theme	inflammation-induced	197:216	arg1	increase					218:225	Chemotherapy- or inflammation-induced increase	180:225	Chemotherapy- or inflammation-induced increase in intestinal permeability	180:252	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	10	5	theme	ME-3	1949:1952	arg1	validity					1924:1931	the validity	1920:1931	the validity of L. fermentum ME-3	1920:1952	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	10	5	theme	ME-3	1949:1952	arg1	tool					1980:1983	a valuable therapeutic tool	1957:1983	a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment	1957:2074	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	9	6	theme	co-culture	1853:1862	arg1	model					1864:1868	the co-culture model	1849:1868	the co-culture model of LPS-induced inflammation	1849:1896	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	0	7	theme	Inflammatory	133:144	arg1	Conditions					146:155	Inflammatory Conditions	133:155	Inflammatory Conditions	133:155	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	10	8	theme	L.	1936:1937	arg1	ME-3					1949:1952	L. fermentum ME-3	1936:1952	L. fermentum ME-3	1936:1952	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	7	9	theme	Caco-2	1287:1292	arg1	cells					1294:1298	Caco-2 cells	1287:1298	Caco-2 cells	1287:1298	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	5	10	theme	barrier	948:954	arg1	monolayer					1009:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	10	theme	barrier	948:954	arg1	model					956:960	an intestinal barrier model	934:960	an intestinal barrier model	934:960	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	9	11	theme	inflammation	1885:1896	arg1	model					1864:1868	the co-culture model	1849:1868	the co-culture model of LPS-induced inflammation	1849:1896	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	0	12	from	Increase	68:75	arg1	Permeability					93:104	Paracellular Permeability	80:104	Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models	80:177	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	0	13	theme	Cell	167:170	arg1	Models					172:177	Caco-2 Cell Models	160:177	Caco-2 Cell Models	160:177	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	5	14	theme	flux	1157:1160	arg1	assays					1162:1167	fluorescent-dextran flux assays	1137:1167	fluorescent-dextran flux assays	1137:1167	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	3	15	theme	intestinal	663:672	arg1	integrity					682:690	intestinal barrier integrity	663:690	intestinal barrier integrity	663:690	Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity.
37047193	5	16	theme	cell	1004:1007	arg1	monolayer					1009:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	16	theme	cell	1004:1007	arg1	model					956:960	an intestinal barrier model	934:960	an intestinal barrier model	934:960	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	17	theme	ME-3-induced	1036:1047	arg1	effect					1049:1054	the ME-3-induced effect	1032:1054	the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays	1032:1167	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	1	18	from	element	274:280	arg1	evolution					293:301	disease evolution	285:301	disease evolution	285:301	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	10	19	theme	leaky	2000:2004	arg1	syndrome					2010:2017	leaky gut syndrome	2000:2017	leaky gut syndrome	2000:2017	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	1	20	from	increase	218:225	arg1	permeability					241:252	intestinal permeability	230:252	intestinal permeability	230:252	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	8	21	theme	paracellular	1614:1625	arg1	permeability					1627:1638	paracellular permeability	1614:1638	paracellular permeability	1614:1638	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	7	22	theme	intestinal	1364:1373	arg1	inflammation					1375:1386	LPS-induced intestinal inflammation	1352:1386	LPS-induced intestinal inflammation	1352:1386	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	23	link	U937-derived	1304:1315	arg1	macrophages					1317:1327	U937-derived macrophages	1304:1327	U937-derived macrophages	1304:1327	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	5	24	theme	confluent	973:981	arg1	monolayer					1009:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	24	theme	confluent	973:981	arg1	model					956:960	an intestinal barrier model	934:960	an intestinal barrier model	934:960	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	3	25	contain	have	580:583	arg1	probiotics					563:572	Some probiotics	558:572	Some probiotics	558:572	Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity.
37047193	3	25	contain	have	580:583	arg2	effect					607:612	a strong re-balancing effect	585:612	a strong re-balancing effect	585:612	Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity.
37047193	4	26	theme	paracellular	787:798	arg1	permeability					800:811	the intestinal paracellular permeability	772:811	the intestinal paracellular permeability	772:811	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	0	27	theme	Paracellular	80:91	arg1	Permeability					93:104	Paracellular Permeability	80:104	Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models	80:177	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	1	28	theme	Chemotherapy-	180:192	arg1	increase					218:225	Chemotherapy- or inflammation-induced increase	180:225	Chemotherapy- or inflammation-induced increase in intestinal permeability	180:252	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	4	29	theme	drug	853:856	arg1	Irinotecan					858:867	the chemotherapeutic drug Irinotecan	832:867	the chemotherapeutic drug Irinotecan	832:867	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	5	30	theme	paracellular	1059:1070	arg1	permeability					1072:1083	paracellular permeability	1059:1083	paracellular permeability	1059:1083	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	2	31	theme	intestinal	452:461	arg1	integrity					471:479	intestinal barrier integrity	452:479	intestinal barrier integrity	452:479	Emerging data strongly support the gut microbiota's role in preserving intestinal barrier integrity, whilst both chemotherapy and gut inflammation alter microbiota composition.
37047193	8	32	from	increment	1601:1609	arg1	permeability					1627:1638	paracellular permeability	1614:1638	paracellular permeability	1614:1638	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	4	33	dep	prevent	764:770	arg1	increase					813:820	increase	813:820	increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS)	813:928	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	9	34	from	increase	1762:1769	arg1	permeability					1787:1798	paracellular permeability	1774:1798	paracellular permeability	1774:1798	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	6	35	theme	tight	1187:1191	arg1	integrity					1174:1182	The integrity	1170:1182	The integrity of tight and adherens junctions	1170:1214	The integrity of tight and adherens junctions was examined by confocal microscopy analysis.
37047193	2	36	theme	gut	511:513	arg1	inflammation					515:526	gut inflammation	511:526	gut inflammation	511:526	Emerging data strongly support the gut microbiota's role in preserving intestinal barrier integrity, whilst both chemotherapy and gut inflammation alter microbiota composition.
37047193	4	37	theme	chemotherapeutic	836:851	arg1	Irinotecan					858:867	the chemotherapeutic drug Irinotecan	832:867	the chemotherapeutic drug Irinotecan	832:867	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	0	38	theme	Protective	0:9	arg1	Effect					11:16	Protective Effect	0:16	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.	0:178	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	7	39	used	used	1334:1337	arg2	models					1342:1347	models	1342:1347	models of LPS-induced intestinal inflammation	1342:1386	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	39	used	used	1334:1337	arg2	co-cultures					1272:1282	Transwell co-cultures	1262:1282	Transwell co-cultures of Caco-2 cells and U937-derived macrophages	1262:1327	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	40	theme	Tumor	1460:1464	arg1	cytokines					1450:1458	the pro-inflammatory cytokines	1429:1458	the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8	1429:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	40	theme	Tumor	1460:1464	arg1	Factor					1475:1480	Tumor Necrosis Factor α	1460:1482	Tumor Necrosis Factor α	1460:1482	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	41	theme	cells	1294:1298	arg1	co-cultures					1272:1282	Transwell co-cultures	1262:1282	Transwell co-cultures of Caco-2 cells and U937-derived macrophages	1262:1327	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	41	theme	cells	1294:1298	arg1	models					1342:1347	models	1342:1347	models of LPS-induced intestinal inflammation	1342:1386	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	42	theme	pro-inflammatory	1433:1448	arg1	Interleukin-6					1485:1497	Interleukin-6	1485:1497	Interleukin-6	1485:1497	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	42	theme	pro-inflammatory	1433:1448	arg1	Interleukin-8					1504:1516	Interleukin-8	1504:1516	Interleukin-8	1504:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	42	theme	pro-inflammatory	1433:1448	arg1	cytokines					1450:1458	the pro-inflammatory cytokines	1429:1458	the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8	1429:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	42	theme	pro-inflammatory	1433:1448	arg1	Factor					1475:1480	Tumor Necrosis Factor α	1460:1482	Tumor Necrosis Factor α	1460:1482	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	8	43	theme	junction	1704:1711	arg1	components					1713:1722	cell junction components	1699:1722	cell junction components	1699:1722	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	0	44	theme	fermentum	41:49	arg1	ME-3					51:54	Limosilactobacillus fermentum ME-3	21:54	Limosilactobacillus fermentum ME-3	21:54	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	1	45	theme	gut	352:354	arg1	conditions					369:378	gut inflammatory conditions	352:378	gut inflammatory conditions	352:378	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	7	46	theme	Transwell	1262:1270	arg1	co-cultures					1272:1282	Transwell co-cultures	1262:1282	Transwell co-cultures of Caco-2 cells and U937-derived macrophages	1262:1327	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	46	theme	Transwell	1262:1270	arg1	models					1342:1347	models	1342:1347	models of LPS-induced intestinal inflammation	1342:1386	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	5	47	theme	fluorescent-dextran	1137:1155	arg1	assays					1162:1167	fluorescent-dextran flux assays	1137:1167	fluorescent-dextran flux assays	1137:1167	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	6	48	theme	microscopy	1241:1250	arg1	analysis					1252:1259	confocal microscopy analysis	1232:1259	confocal microscopy analysis	1232:1259	The integrity of tight and adherens junctions was examined by confocal microscopy analysis.
37047193	10	49	theme	available	2047:2055	arg1	treatment					2066:2074	an available specific treatment	2044:2074	an available specific treatment	2044:2074	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	5	50	from	effect	1049:1054	arg1	permeability					1072:1083	paracellular permeability	1059:1083	paracellular permeability	1059:1083	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	1	51	theme	severe	267:272	arg1	element					274:280	a severe element	265:280	a severe element in disease evolution	265:301	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	5	52	theme	electrical	1104:1113	arg1	resistance					1115:1124	transepithelial electrical resistance	1088:1124	transepithelial electrical resistance (TEER)	1088:1131	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	52	theme	electrical	1104:1113	arg1	TEER					1127:1130	TEER	1127:1130	TEER	1127:1130	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	3	53	theme	strong	587:592	arg1	effect					607:612	a strong re-balancing effect	585:612	a strong re-balancing effect	585:612	Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity.
37047193	8	54	dep	expression	1668:1677	arg1	the					1664:1666	the	1664:1666	the	1664:1666	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	4	55	dep	Limosilactobacillus	725:743	arg1	fermentum					745:753	fermentum	745:753	fermentum	745:753	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	2	56	theme	gut	416:418	arg1	microbiota					420:429	the gut microbiota's	412:431	the gut microbiota's role in preserving intestinal barrier integrity	412:479	Emerging data strongly support the gut microbiota's role in preserving intestinal barrier integrity, whilst both chemotherapy and gut inflammation alter microbiota composition.
37047193	6	57	theme	adherens	1197:1204	arg1	integrity					1174:1182	The integrity	1170:1182	The integrity of tight and adherens junctions	1170:1214	The integrity of tight and adherens junctions was examined by confocal microscopy analysis.
37047193	9	58	theme	cytokines	1836:1844	arg1	release					1808:1814	the release	1804:1814	the release of pro-inflammatory cytokines	1804:1844	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	9	58	theme	cytokines	1836:1844	arg1	increase					1762:1769	the increase	1758:1769	the increase in paracellular permeability	1758:1798	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	7	59	from	effect	1400:1405	arg1	release					1418:1424	release	1418:1424	release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8	1418:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	4	60	theme	Limosilactobacillus	725:743	arg1	ME-3					755:758	Limosilactobacillus fermentum ME-3	725:758	Limosilactobacillus fermentum ME-3	725:758	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	5	61	used	used	966:969	arg2	we					963:964	we	963:964	we	963:964	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	10	62	theme	fermentum	1939:1947	arg1	ME-3					1949:1952	L. fermentum ME-3	1936:1952	L. fermentum ME-3	1936:1952	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	1	63	theme	intestinal	230:239	arg1	permeability					241:252	intestinal permeability	230:252	intestinal permeability	230:252	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	5	64	theme	polarized	987:995	arg1	monolayer					1009:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	64	theme	polarized	987:995	arg1	model					956:960	an intestinal barrier model	934:960	an intestinal barrier model	934:960	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	0	65	theme	Caco-2	160:165	arg1	Models					172:177	Caco-2 Cell Models	160:177	Caco-2 Cell Models	160:177	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	3	66	theme	barrier	674:680	arg1	integrity					682:690	intestinal barrier integrity	663:690	intestinal barrier integrity	663:690	Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity.
37047193	5	67	theme	intestinal	937:946	arg1	monolayer					1009:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	67	theme	intestinal	937:946	arg1	model					956:960	an intestinal barrier model	934:960	an intestinal barrier model	934:960	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	9	68	from	release	1808:1814	arg1	permeability					1787:1798	paracellular permeability	1774:1798	paracellular permeability	1774:1798	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	9	69	theme	LPS-induced	1873:1883	arg1	inflammation					1885:1896	LPS-induced inflammation	1873:1896	LPS-induced inflammation	1873:1896	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	8	70	theme	IRI-induced	1589:1599	arg1	increment					1601:1609	the IRI-induced increment	1585:1609	the IRI-induced increment in paracellular permeability	1585:1638	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	10	71	theme	valuable	1959:1966	arg1	validity					1924:1931	the validity	1920:1931	the validity of L. fermentum ME-3	1920:1952	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	10	71	theme	valuable	1959:1966	arg1	tool					1980:1983	a valuable therapeutic tool	1957:1983	a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment	1957:2074	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	5	72	theme	Caco-2	997:1002	arg1	monolayer					1009:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	a confluent and polarized Caco-2 cell monolayer	971:1017	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	72	theme	Caco-2	997:1002	arg1	model					956:960	an intestinal barrier model	934:960	an intestinal barrier model	934:960	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	10	73	theme	gut	2006:2008	arg1	syndrome					2010:2017	leaky gut syndrome	2000:2017	leaky gut syndrome	2000:2017	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	7	74	theme	LPS-induced	1352:1362	arg1	inflammation					1375:1386	LPS-induced intestinal inflammation	1352:1386	LPS-induced intestinal inflammation	1352:1386	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	4	75	theme	intestinal	776:785	arg1	permeability					800:811	the intestinal paracellular permeability	772:811	the intestinal paracellular permeability	772:811	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	7	76	theme	macrophages	1317:1327	arg1	co-cultures					1272:1282	Transwell co-cultures	1262:1282	Transwell co-cultures of Caco-2 cells and U937-derived macrophages	1262:1327	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	76	theme	macrophages	1317:1327	arg1	models					1342:1347	models	1342:1347	models of LPS-induced intestinal inflammation	1342:1386	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	77	theme	Necrosis	1466:1473	arg1	cytokines					1450:1458	the pro-inflammatory cytokines	1429:1458	the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8	1429:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	77	theme	Necrosis	1466:1473	arg1	Factor					1475:1480	Tumor Necrosis Factor α	1460:1482	Tumor Necrosis Factor α	1460:1482	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	4	78	theme	inflammatory	875:886	arg1	lipopolysaccharide					905:922	lipopolysaccharide	905:922	lipopolysaccharide (LPS)	905:928	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	4	78	theme	inflammatory	875:886	arg1	stimuli					888:894	inflammatory stimuli	875:894	inflammatory stimuli	875:894	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	7	79	theme	cytokines	1450:1458	arg1	release					1418:1424	release	1418:1424	release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8	1418:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	8	80	theme	cell	1699:1702	arg1	components					1713:1722	cell junction components	1699:1722	cell junction components	1699:1722	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	0	81	theme	Limosilactobacillus	21:39	arg1	ME-3					51:54	Limosilactobacillus fermentum ME-3	21:54	Limosilactobacillus fermentum ME-3	21:54	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	1	82	theme	colorectal	330:339	arg1	cancer					341:346	colorectal cancer	330:346	colorectal cancer	330:346	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	4	83	dep	increase	813:820	arg1	caused					822:827	caused	822:827	increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS)	813:928	In this study, we asked whether Limosilactobacillus fermentum ME-3 can prevent the intestinal paracellular permeability increase caused by the chemotherapeutic drug Irinotecan or by inflammatory stimuli, such as lipopolysaccharide (LPS).
37047193	8	84	theme	components	1713:1722	arg1	expression					1668:1677	expression	1668:1677	expression	1668:1677	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	8	84	theme	components	1713:1722	arg1	localization					1683:1694	localization	1683:1694	localization	1683:1694	The results demonstrate that ME-3 prevents the IRI-induced increment in paracellular permeability, possibly by modulating the expression and localization of cell junction components.
37047193	6	85	dep	tight	1187:1191	arg1	junctions					1206:1214	junctions	1206:1214	junctions	1206:1214	The integrity of tight and adherens junctions was examined by confocal microscopy analysis.
37047193	0	86	theme	ME-3	51:54	arg1	Effect					11:16	Protective Effect	0:16	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.	0:178	Protective Effect of Limosilactobacillus fermentum ME-3 against the Increase in Paracellular Permeability Induced by Chemotherapy or Inflammatory Conditions in Caco-2 Cell Models.
37047193	7	87	dep	cytokines	1450:1458	arg1	Interleukin-6					1485:1497	Interleukin-6	1485:1497	Interleukin-6	1485:1497	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	87	dep	cytokines	1450:1458	arg1	Interleukin-8					1504:1516	Interleukin-8	1504:1516	Interleukin-8	1504:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	87	dep	cytokines	1450:1458	arg1	cytokines					1450:1458	the pro-inflammatory cytokines	1429:1458	the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8	1429:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	7	87	dep	cytokines	1450:1458	arg1	Factor					1475:1480	Tumor Necrosis Factor α	1460:1482	Tumor Necrosis Factor α	1460:1482	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	2	88	theme	microbiota	534:543	arg1	composition					545:555	microbiota composition	534:555	microbiota composition	534:555	Emerging data strongly support the gut microbiota's role in preserving intestinal barrier integrity, whilst both chemotherapy and gut inflammation alter microbiota composition.
37047193	2	89	theme	barrier	463:469	arg1	integrity					471:479	intestinal barrier integrity	452:479	intestinal barrier integrity	452:479	Emerging data strongly support the gut microbiota's role in preserving intestinal barrier integrity, whilst both chemotherapy and gut inflammation alter microbiota composition.
37047193	1	90	theme	inflammatory	356:367	arg1	conditions					369:378	gut inflammatory conditions	352:378	gut inflammatory conditions	352:378	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
37047193	7	91	theme	ME-3	1410:1413	arg1	effect					1400:1405	the effect	1396:1405	the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8	1396:1516	Transwell co-cultures of Caco-2 cells and U937-derived macrophages were used as models of LPS-induced intestinal inflammation to test the effect of ME-3 on release of the pro-inflammatory cytokines Tumor Necrosis Factor α, Interleukin-6, and Interleukin-8, was measured by ELISA.
37047193	10	92	theme	specific	2057:2064	arg1	treatment					2066:2074	an available specific treatment	2044:2074	an available specific treatment	2044:2074	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	5	93	theme	transepithelial	1088:1102	arg1	resistance					1115:1124	transepithelial electrical resistance	1088:1124	transepithelial electrical resistance (TEER)	1088:1131	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	5	93	theme	transepithelial	1088:1102	arg1	TEER					1127:1130	TEER	1127:1130	TEER	1127:1130	As an intestinal barrier model, we used a confluent and polarized Caco-2 cell monolayer and assessed the ME-3-induced effect on paracellular permeability by transepithelial electrical resistance (TEER) and fluorescent-dextran flux assays.
37047193	10	94	theme	therapeutic	1968:1978	arg1	validity					1924:1931	the validity	1920:1931	the validity of L. fermentum ME-3	1920:1952	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	10	94	theme	therapeutic	1968:1978	arg1	tool					1980:1983	a valuable therapeutic tool	1957:1983	a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment	1957:2074	Our findings sustain the validity of L. fermentum ME-3 as a valuable therapeutic tool for preventing leaky gut syndrome, still currently without an available specific treatment.
37047193	6	95	theme	confocal	1232:1239	arg1	analysis					1252:1259	confocal microscopy analysis	1232:1259	confocal microscopy analysis	1232:1259	The integrity of tight and adherens junctions was examined by confocal microscopy analysis.
37047193	2	96	theme	Emerging	381:388	arg1	data					390:393	Emerging data	381:393	Emerging data	381:393	Emerging data strongly support the gut microbiota's role in preserving intestinal barrier integrity, whilst both chemotherapy and gut inflammation alter microbiota composition.
37047193	9	97	theme	paracellular	1774:1785	arg1	permeability					1787:1798	paracellular permeability	1774:1798	paracellular permeability	1774:1798	In addition, ME-3 inhibited both the increase in paracellular permeability and the release of pro-inflammatory cytokines in the co-culture model of LPS-induced inflammation.
37047193	3	98	theme	re-balancing	594:605	arg1	effect					607:612	a strong re-balancing effect	585:612	a strong re-balancing effect	585:612	Some probiotics might have a strong re-balancing effect on the gut microbiota, also positively affecting intestinal barrier integrity.
37047193	1	99	theme	disease	285:291	arg1	evolution					293:301	disease evolution	285:301	disease evolution	285:301	Chemotherapy- or inflammation-induced increase in intestinal permeability represents a severe element in disease evolution in patients suffering from colorectal cancer and gut inflammatory conditions.
35629459	6	0	with	filler	1158:1163	arg1	propolis					1170:1177	propolis	1170:1177	propolis	1170:1177	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	10	1	theme	effective	1896:1904	arg1	system					1860:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	1	theme	effective	1896:1904	arg1	stabilizer					1924:1933	an effective and ecological UV stabilizer	1893:1933	an effective and ecological UV stabilizer	1893:1933	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	9	2	theme	photo-	1706:1711	arg1	inhibition					1692:1701	the inhibition	1688:1701	the inhibition of photo- and biodegradation of WPC materials	1688:1747	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	2	3	theme	innovative	297:306	arg1	modifier					331:338	an innovative hybrid propolis-silane modifier	294:338	an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix	294:483	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	9	4	theme	biodegradation	1717:1730	arg1	inhibition					1692:1701	the inhibition	1688:1701	the inhibition of photo- and biodegradation of WPC materials	1688:1747	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	7	5	from	changes	1310:1316	arg1	structure					1289:1297	the supermolecular structure	1270:1297	the supermolecular structure	1270:1297	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	7	5	from	changes	1310:1316	arg1	intensity					1325:1333	the intensity	1321:1333	the intensity of the bands characteristic of polysaccharides and lignin	1321:1391	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	8	6	mod	modified	1456:1463	arg3	propolis					1470:1477	propolis	1470:1477	propolis	1470:1477	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	8	6	mod	modified	1456:1463	arg1	wood					1433:1436	pine wood	1428:1436	pine wood that had not been modified with propolis	1428:1477	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	9	7	theme	WPC	1735:1737	arg1	biodegradation					1717:1730	biodegradation	1717:1730	biodegradation of WPC	1717:1737	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	10	8	theme	applied	1831:1837	arg1	agent					1961:1965	an antifungal agent	1947:1965	an antifungal agent	1947:1965	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	8	theme	applied	1831:1837	arg1	system					1860:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	8	theme	applied	1831:1837	arg1	stabilizer					1924:1933	an effective and ecological UV stabilizer	1893:1933	an effective and ecological UV stabilizer	1893:1933	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	2	9	theme	fungal	394:399	arg1	attack					401:406	fungal attack	394:406	fungal attack	394:406	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	10	10	theme	modifying	1850:1858	arg1	agent					1961:1965	an antifungal agent	1947:1965	an antifungal agent	1947:1965	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	10	theme	modifying	1850:1858	arg1	system					1860:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	10	theme	modifying	1850:1858	arg1	stabilizer					1924:1933	an effective and ecological UV stabilizer	1893:1933	an effective and ecological UV stabilizer	1893:1933	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	5	11	theme	composite	950:958	arg1	materials					960:968	composite materials	950:968	composite materials	950:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	4	12	dep	fungi	611:615	arg1	puteana					684:690	brown-rot fungus Coniophora puteana	656:690	brown-rot fungus Coniophora puteana	656:690	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	12	dep	fungi	611:615	arg1	versicolor					644:653	white-rot fungus Coriolus versicolor	618:653	white-rot fungus Coriolus versicolor	618:653	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	12	dep	fungi	611:615	arg1	fungi					611:615	the fungi	607:615	the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus	607:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	12	dep	fungi	611:615	arg1	fungus					706:711	soft-rot fungus	697:711	soft-rot fungus	697:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	2	13	theme	propolis-silane	315:329	arg1	modifier					331:338	an innovative hybrid propolis-silane modifier	294:338	an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix	294:483	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	8	14	theme	significant	1480:1490	arg1	changes					1492:1498	significant changes	1480:1498	significant changes in the crystalline structure of polymer composites	1480:1549	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	4	15	theme	fungus	666:671	arg1	puteana					684:690	brown-rot fungus Coniophora puteana	656:690	brown-rot fungus Coniophora puteana	656:690	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	15	theme	fungus	666:671	arg1	fungi					611:615	the fungi	607:615	the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus	607:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	3	16	with	composites	500:509	arg1	content					530:536	30% pine wood content	516:536	30% pine wood content	516:536	Polypropylene composites with 30% pine wood content were obtained by extrusion and pressing.
35629459	9	17	dep	photo-	1706:1711	arg1	materials					1739:1747	materials	1739:1747	materials	1739:1747	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	9	18	theme	slight	1773:1778	arg1	deterioration					1780:1792	a slight deterioration	1771:1792	a slight deterioration in selected strength parameters	1771:1824	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	8	19	theme	crystalline	1507:1517	arg1	structure					1519:1527	the crystalline structure	1503:1527	the crystalline structure of polymer composites	1503:1549	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	5	20	theme	materials	960:968	arg1	destruction					935:945	the surface destruction	923:945	the surface destruction of composite materials	923:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	1	21	theme	many	165:168	arg1	advantages					170:179	their many advantages	159:179	their many advantages	159:179	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	9	22	from	deterioration	1780:1792	arg1	parameters					1815:1824	selected strength parameters	1797:1824	selected strength parameters	1797:1824	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	2	23	theme	interfacial	435:445	arg1	adhesion					447:454	good interfacial adhesion	430:454	good interfacial adhesion of the filler-polymer matrix	430:483	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	7	24	theme	supermolecular	1274:1287	arg1	structure					1289:1297	the supermolecular structure	1270:1297	the supermolecular structure	1270:1297	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	9	25	theme	strength	1806:1813	arg1	parameters					1815:1824	selected strength parameters	1797:1824	selected strength parameters	1797:1824	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	8	26	theme	composites	1540:1549	arg1	structure					1519:1527	the crystalline structure	1503:1527	the crystalline structure of polymer composites	1503:1549	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	5	27	theme	factors	912:918	arg1	influence					894:902	the influence	890:902	the influence of both factors on the surface destruction of composite materials	890:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	6	28	dep	reflectance-Fourier	1011:1029	arg1	transform					1031:1039	transform	1031:1039	transform infrared spectroscopy	1031:1061	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	5	29	from	influence	894:902	arg1	destruction					935:945	the surface destruction	923:945	the surface destruction of composite materials	923:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	6	30	theme	attenuated	994:1003	arg1	reflectance-Fourier					1011:1029	attenuated total reflectance-Fourier	994:1029	attenuated total reflectance-Fourier transform infrared spectroscopy	994:1061	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	2	31	theme	good	430:433	arg1	adhesion					447:454	good interfacial adhesion	430:454	good interfacial adhesion of the filler-polymer matrix	430:483	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	2	32	theme	filler-polymer	463:476	arg1	matrix					478:483	the filler-polymer matrix	459:483	the filler-polymer matrix	459:483	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	0	33	theme	Bioactive	0:8	arg1	Propolis-Silane					10:24	Bioactive Propolis-Silane	0:24	Bioactive Propolis-Silane	0:24	Bioactive Propolis-Silane System as Antifungal Agent in Lignocellulosic-Polymer Composites.
35629459	1	34	theme	renewable	116:124	arg1	fillers					142:148	renewable lignocellulosic fillers	116:148	renewable lignocellulosic fillers	116:148	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	6	35	theme	significant	1097:1107	arg1	effect					1109:1114	a significant effect	1095:1114	a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi	1095:1215	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	8	36	theme	decay	1590:1594	arg1	processes					1596:1604	decay processes	1590:1604	decay processes	1590:1604	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	3	37	theme	Polypropylene	486:498	arg1	composites					500:509	Polypropylene composites	486:509	Polypropylene composites with 30% pine wood content	486:536	Polypropylene composites with 30% pine wood content were obtained by extrusion and pressing.
35629459	10	38	theme	ecological	1910:1919	arg1	system					1860:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	38	theme	ecological	1910:1919	arg1	stabilizer					1924:1933	an effective and ecological UV stabilizer	1893:1933	an effective and ecological UV stabilizer	1893:1933	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	0	39	theme	Antifungal	36:45	arg1	Agent					47:51	Antifungal Agent	36:51	Antifungal Agent in Lignocellulosic-Polymer Composites	36:89	Bioactive Propolis-Silane System as Antifungal Agent in Lignocellulosic-Polymer Composites.
35629459	9	40	theme	propolis-silane	1641:1655	arg1	system					1664:1669	the propolis-silane hybrid system	1637:1669	the propolis-silane hybrid system wood	1637:1674	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	7	41	theme	bands	1342:1346	arg1	intensity					1325:1333	the intensity	1321:1333	the intensity of the bands characteristic of polysaccharides and lignin	1321:1391	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	5	42	theme	samples	780:786	arg1	tests					771:775	biological tests	760:775	biological tests of samples that had been previously exposed to UV radiation	760:835	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	3	43	theme	%	518:518	arg1	content					530:536	30% pine wood content	516:536	30% pine wood content	516:536	Polypropylene composites with 30% pine wood content were obtained by extrusion and pressing.
35629459	9	44	theme	system	1664:1669	arg1	wood					1671:1674	the propolis-silane hybrid system wood	1637:1674	the propolis-silane hybrid system wood	1637:1674	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	6	45	theme	filler	1158:1163	arg1	modification					1123:1134	the modification	1119:1134	the modification of the lignocellulose filler with propolis	1119:1177	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	10	46	dep	stabilizer	1924:1933	arg1	both					1888:1891	both	1888:1891	both	1888:1891	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	6	47	theme	mycological	1068:1078	arg1	studies					1080:1086	mycological studies	1068:1086	mycological studies	1068:1086	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	4	48	theme	fungus	628:633	arg1	versicolor					644:653	white-rot fungus Coriolus versicolor	618:653	white-rot fungus Coriolus versicolor	618:653	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	48	theme	fungus	628:633	arg1	fungi					611:615	the fungi	607:615	the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus	607:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	10	49	theme	antifungal	1950:1959	arg1	agent					1961:1965	an antifungal agent	1947:1965	an antifungal agent	1947:1965	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	49	theme	antifungal	1950:1959	arg1	system					1860:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	3	50	theme	wood	525:528	arg1	content					530:536	30% pine wood content	516:536	30% pine wood content	516:536	Polypropylene composites with 30% pine wood content were obtained by extrusion and pressing.
35629459	1	51	theme	applications	266:277	arg1	terms					248:252	terms	248:252	terms of external applications	248:277	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	6	52	theme	modification	1123:1134	arg1	effect					1109:1114	a significant effect	1095:1114	a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi	1095:1215	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	2	53	theme	hybrid	308:313	arg1	modifier					331:338	an innovative hybrid propolis-silane modifier	294:338	an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix	294:483	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	4	54	theme	Coniophora	673:682	arg1	puteana					684:690	brown-rot fungus Coniophora puteana	656:690	brown-rot fungus Coniophora puteana	656:690	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	54	theme	Coniophora	673:682	arg1	fungi					611:615	the fungi	607:615	the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus	607:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	10	55	theme	innovative	1839:1848	arg1	agent					1961:1965	an antifungal agent	1947:1965	an antifungal agent	1947:1965	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	55	theme	innovative	1839:1848	arg1	system					1860:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	55	theme	innovative	1839:1848	arg1	stabilizer					1924:1933	an effective and ecological UV stabilizer	1893:1933	an effective and ecological UV stabilizer	1893:1933	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	6	56	theme	X-ray	975:979	arg1	diffraction					981:991	The X-ray diffraction	971:991	The X-ray diffraction	971:991	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	1	57	theme	major	228:232	arg1	biodegradation					201:214	biodegradation	201:214	biodegradation	201:214	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	1	57	theme	major	228:232	arg1	limitation					234:243	a major limitation	226:243	a major limitation in terms of external applications	226:277	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	1	58	with	composites	100:109	arg1	fillers					142:148	renewable lignocellulosic fillers	116:148	renewable lignocellulosic fillers	116:148	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	4	59	theme	brown-rot	656:664	arg1	puteana					684:690	brown-rot fungus Coniophora puteana	656:690	brown-rot fungus Coniophora puteana	656:690	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	59	theme	brown-rot	656:664	arg1	fungi					611:615	the fungi	607:615	the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus	607:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	1	60	from	limitation	234:243	arg1	terms					248:252	terms	248:252	terms of external applications	248:277	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	7	61	from	changes	1259:1265	arg1	structure					1289:1297	the supermolecular structure	1270:1297	the supermolecular structure	1270:1297	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	7	61	from	changes	1259:1265	arg1	intensity					1325:1333	the intensity	1321:1333	the intensity of the bands characteristic of polysaccharides and lignin	1321:1391	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	4	62	dep	fungus	628:633	arg1	Coriolus					635:642	Coriolus	635:642	Coriolus	635:642	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	5	63	theme	surface	927:933	arg1	destruction					935:945	the surface destruction	923:945	the surface destruction of composite materials	923:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	8	64	theme	polymer	1532:1538	arg1	composites					1540:1549	polymer composites	1532:1549	polymer composites	1532:1549	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	5	65	theme	influence	894:902	arg1	determination					873:885	the determination	869:885	the determination of the influence of both factors on the surface destruction of composite materials	869:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	9	66	theme	selected	1797:1804	arg1	parameters					1815:1824	selected strength parameters	1797:1824	selected strength parameters	1797:1824	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	4	67	theme	soft-rot	697:704	arg1	fungi					611:615	the fungi	607:615	the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus	607:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	67	theme	soft-rot	697:704	arg1	fungus					706:711	soft-rot fungus	697:711	soft-rot fungus	697:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	8	68	from	changes	1492:1498	arg1	structure					1519:1527	the crystalline structure	1503:1527	the crystalline structure of polymer composites	1503:1549	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	5	69	from	determination	873:885	arg1	destruction					935:945	the surface destruction	923:945	the surface destruction of composite materials	923:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	8	70	contain	containing	1417:1426	arg2	wood					1433:1436	pine wood	1428:1436	pine wood that had not been modified with propolis	1428:1477	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	8	70	contain	containing	1417:1426	arg1	systems					1409:1415	systems	1409:1415	systems containing pine wood that had not been modified with propolis	1409:1477	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	6	71	dep	transform	1031:1039	arg1	infrared					1041:1048	infrared	1041:1048	transform infrared spectroscopy	1031:1061	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	6	72	theme	total	1005:1009	arg1	reflectance-Fourier					1011:1029	attenuated total reflectance-Fourier	994:1029	attenuated total reflectance-Fourier transform infrared spectroscopy	994:1061	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	5	73	theme	biological	760:769	arg1	tests					771:775	biological tests	760:775	biological tests of samples that had been previously exposed to UV radiation	760:835	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	1	74	theme	Polymer	92:98	arg1	composites					100:109	Polymer composites	92:109	Polymer composites with renewable lignocellulosic fillers	92:148	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	7	75	theme	lignin	1386:1391	arg1	characteristic					1348:1361	characteristic	1348:1361	characteristic	1348:1361	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	0	76	from	Agent	47:51	arg1	Composites					80:89	Lignocellulosic-Polymer Composites	56:89	Lignocellulosic-Polymer Composites	56:89	Bioactive Propolis-Silane System as Antifungal Agent in Lignocellulosic-Polymer Composites.
35629459	5	77	theme	UV	824:825	arg1	radiation					827:835	UV radiation	824:835	UV radiation	824:835	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	5	78	from	destruction	935:945	arg1	determination					873:885	the determination	869:885	the determination of the influence of both factors on the surface destruction of composite materials	869:968	Additionally, biological tests of samples that had been previously exposed to UV radiation were carried out, which allowed the determination of the influence of both factors on the surface destruction of composite materials.
35629459	7	79	theme	polysaccharides	1366:1380	arg1	characteristic					1348:1361	characteristic	1348:1361	characteristic	1348:1361	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	8	80	theme	processes	1596:1604	arg1	progress					1578:1585	the progress	1574:1585	the progress of decay processes	1574:1604	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	8	81	theme	systems	1409:1415	arg1	case					1401:1404	the case	1397:1404	the case of systems containing pine wood that had not been modified with propolis	1397:1477	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	1	82	theme	lignocellulosic	126:140	arg1	fillers					142:148	renewable lignocellulosic fillers	116:148	renewable lignocellulosic fillers	116:148	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
35629459	7	83	theme	characteristic	1348:1361	arg1	bands					1342:1346	the bands	1338:1346	the bands characteristic of polysaccharides and lignin	1338:1391	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	10	84	theme	UV	1921:1922	arg1	system					1860:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system	1827:1865	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	10	84	theme	UV	1921:1922	arg1	stabilizer					1924:1933	an effective and ecological UV stabilizer	1893:1933	an effective and ecological UV stabilizer	1893:1933	The applied innovative modifying system can therefore act as both an effective and ecological UV stabilizer, as well as an antifungal agent.
35629459	8	85	theme	pine	1428:1431	arg1	wood					1433:1436	pine wood	1428:1436	pine wood that had not been modified with propolis	1428:1477	In the case of systems containing pine wood that had not been modified with propolis, significant changes in the crystalline structure of polymer composites were noted, indicating the progress of decay processes.
35629459	6	86	theme	lignocellulose	1143:1156	arg1	filler					1158:1163	the lignocellulose filler	1139:1163	the lignocellulose filler with propolis	1139:1177	The X-ray diffraction, attenuated total reflectance-Fourier transform infrared spectroscopy, and mycological studies showed a significant effect of the modification of the lignocellulose filler with propolis on increasing the resistance to fungi.
35629459	0	87	theme	Lignocellulosic-Polymer	56:78	arg1	Composites					80:89	Lignocellulosic-Polymer Composites	56:89	Lignocellulosic-Polymer Composites	56:89	Bioactive Propolis-Silane System as Antifungal Agent in Lignocellulosic-Polymer Composites.
35629459	9	88	theme	hybrid	1657:1662	arg1	system					1664:1669	the propolis-silane hybrid system	1637:1669	the propolis-silane hybrid system wood	1637:1674	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	2	89	theme	matrix	478:483	arg1	adhesion					447:454	good interfacial adhesion	430:454	good interfacial adhesion of the filler-polymer matrix	430:483	The work uses an innovative hybrid propolis-silane modifier in order to simultaneously increase the resistance to fungal attack, as well as to ensure good interfacial adhesion of the filler-polymer matrix.
35629459	3	90	theme	pine	520:523	arg1	content					530:536	30% pine wood content	516:536	30% pine wood content	516:536	Polypropylene composites with 30% pine wood content were obtained by extrusion and pressing.
35629459	9	91	theme	wood	1671:1674	arg1	modification					1621:1632	the modification	1617:1632	the modification of the propolis-silane hybrid system wood	1617:1674	Moreover, the modification of the propolis-silane hybrid system wood resulted in the inhibition of photo- and biodegradation of WPC materials, as evidenced only by a slight deterioration in selected strength parameters.
35629459	4	92	theme	white-rot	618:626	arg1	versicolor					644:653	white-rot fungus Coriolus versicolor	618:653	white-rot fungus Coriolus versicolor	618:653	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	4	92	theme	white-rot	618:626	arg1	fungi					611:615	the fungi	607:615	the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus	607:711	The samples were exposed to the fungi: white-rot fungus Coriolus versicolor, brown-rot fungus Coniophora puteana, and soft-rot fungus Chaetomium globosum for 8 weeks.
35629459	3	93	theme	30	516:517	arg1	%					518:518	%	518:518	%	518:518	Polypropylene composites with 30% pine wood content were obtained by extrusion and pressing.
35629459	7	94	theme	slight	1303:1308	arg1	changes					1310:1316	slight changes	1303:1316	slight changes in the intensity of the bands characteristic of polysaccharides and lignin	1303:1391	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	7	95	theme	Such	1218:1221	arg1	composites					1223:1232	Such composites	1218:1232	Such composites	1218:1232	Such composites were characterized by no changes in the supermolecular structure and slight changes in the intensity of the bands characteristic of polysaccharides and lignin.
35629459	1	96	theme	external	257:264	arg1	applications					266:277	external applications	257:277	external applications	257:277	Polymer composites with renewable lignocellulosic fillers, despite their many advantages, are susceptible to biodegradation, which is a major limitation in terms of external applications.
36364913	8	0	from	decrease	1344:1351	arg1	TMAO					1363:1366	plasma TMAO	1356:1366	plasma TMAO	1356:1366	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	8	0	from	decrease	1344:1351	arg1	levels					1399:1404	fecal SCFAs levels	1387:1404	fecal SCFAs levels in mice treated with parsley and rosemary essential oils	1387:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	6	1	theme	oxidative	1143:1151	arg1	stress					1153:1158	oxidative stress	1143:1158	oxidative stress	1143:1158	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	6	1	theme	oxidative	1143:1151	arg1	effects					1005:1011	the effects	1001:1011	the effects on gut microbiota composition and abundance	1001:1055	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	3	2	theme	gut	511:513	arg1	microbiota					515:524	the gut microbiota	507:524	the gut microbiota of these patients	507:542	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	8	3	from	increase	1375:1382	arg1	TMAO					1363:1366	plasma TMAO	1356:1366	plasma TMAO	1356:1366	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	8	3	from	increase	1375:1382	arg1	levels					1399:1404	fecal SCFAs levels	1387:1404	fecal SCFAs levels in mice treated with parsley and rosemary essential oils	1387:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	1	4	theme	health	239:244	arg1	problems					246:253	major health problems worldwide	233:263	major health problems worldwide	233:263	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	4	5	theme	oil	626:628	arg1	prebiotics					777:786	prebiotics	777:786	prebiotics	777:786	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	5	theme	oil	626:628	arg1	emulsions					630:638	Essential oil emulsions	616:638	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis)	616:739	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	5	theme	oil	626:628	arg1	nutraceuticals					758:771	nutraceuticals	758:771	nutraceuticals	758:771	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	1	6	theme	heart	170:174	arg1	disease					176:182	Ischemic heart disease	161:182	Ischemic heart disease (IHD)	161:188	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	1	6	theme	heart	170:174	arg1	IHD					185:187	IHD	185:187	IHD	185:187	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	6	7	theme	disease	1117:1123	arg1	metabolites					1068:1078	microbial metabolites	1058:1078	microbial metabolites	1058:1078	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	6	7	theme	disease	1117:1123	arg1	markers					1091:1097	plasma markers	1084:1097	plasma markers	1084:1097	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	6	7	theme	disease	1117:1123	arg1	effects					1005:1011	the effects	1001:1011	the effects on gut microbiota composition and abundance	1001:1055	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	12	8	theme	metabolic	2072:2080	arg1	factors					2082:2088	cardiovascular and metabolic factors	2053:2088	cardiovascular and metabolic factors	2053:2088	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	3	9	theme	patients	535:542	arg1	effects					562:568	the prebiotic effects	548:568	the prebiotic effects of some components of the Mediterranean diet	548:613	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	3	9	theme	patients	535:542	arg1	microbiota					515:524	the gut microbiota	507:524	the gut microbiota of these patients	507:542	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	8	10	from	levels	1399:1404	arg1	mice					1409:1412	mice	1409:1412	mice treated with parsley and rosemary essential oils	1409:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	5	11	theme	gut	830:832	arg1	microbiota					834:843	gut microbiota	830:843	gut microbiota derived from that of patients with IHD and T2DM	830:891	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	11	12	theme	essential	1870:1878	arg1	emulsion					1884:1891	the essential oil emulsion	1866:1891	the essential oil emulsion	1866:1891	Finally, there was a decrease in protein carbonyls and pentosidine according to the essential oil emulsion.
36364913	9	13	theme	essential	1541:1549	arg1	oils					1551:1554	savory and parsley essential oils	1522:1554	savory and parsley essential oils	1522:1554	Thrombomodulin levels were increased in mice treated with savory and parsley essential oils.
36364913	0	14	theme	Ischemic	108:115	arg1	Disease					123:129	Ischemic Heart Disease	108:129	Ischemic Heart Disease	108:129	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	10	15	theme	rosemary	1593:1600	arg1	oils					1612:1615	rosemary essential oils	1593:1615	rosemary essential oils	1593:1615	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	10	16	theme	chemokines	1750:1759	arg1	levels					1740:1745	increased levels	1730:1745	increased levels of chemokines (CXCL1, CCL2 and CCL11)	1730:1783	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	1	17	theme	type-2	194:199	arg1	T2DM					220:223	T2DM	220:223	T2DM	220:223	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	1	17	theme	type-2	194:199	arg1	mellitus					210:217	type-2 diabetes mellitus	194:217	type-2 diabetes mellitus (T2DM)	194:224	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	0	18	from	Diet	60:63	arg1	Effects					11:17	Beneficial Effects	0:17	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus	0:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	0	18	from	Diet	60:63	arg1	Oils					32:35	Essential Oils	22:35	Essential Oils from the Mediterranean Diet	22:63	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	12	19	theme	savory	1989:1994	arg1	oils					1972:1975	essential oils	1962:1975	essential oils of parsley, savory and rosemary as prebiotics	1962:2021	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	0	20	theme	Type-2	135:140	arg1	Mellitus					151:158	Type-2 Diabetes Mellitus	135:158	Type-2 Diabetes Mellitus	135:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	0	21	from	Metabolites	93:103	arg1	Disease					123:129	Ischemic Heart Disease	108:129	Ischemic Heart Disease	108:129	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	0	21	from	Metabolites	93:103	arg1	Mellitus					151:158	Type-2 Diabetes Mellitus	135:158	Type-2 Diabetes Mellitus	135:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	8	22	theme	parsley	1427:1433	arg1	oils					1458:1461	parsley and rosemary essential oils	1427:1461	parsley and rosemary essential oils	1427:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	12	23	theme	rosemary	2000:2007	arg1	oils					1972:1975	essential oils	1962:1975	essential oils of parsley, savory and rosemary as prebiotics	1962:2021	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	12	24	theme	IHD	2159:2161	arg1	risk					2163:2166	IHD risk	2159:2166	IHD risk in patients affected by T2DM	2159:2195	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	8	25	theme	rosemary	1439:1446	arg1	oils					1458:1461	parsley and rosemary essential oils	1427:1461	parsley and rosemary essential oils	1427:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	7	26	theme	oil	1195:1197	arg1	emulsions					1199:1207	essential oil emulsions	1185:1207	essential oil emulsions in mice supplemented with L-carnitine	1185:1245	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	2	27	theme	metabolic	443:451	arg1	diseases					453:460	cardiovascular and metabolic diseases	424:460	cardiovascular and metabolic diseases	424:460	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	12	28	theme	parsley	1980:1986	arg1	oils					1972:1975	essential oils	1962:1975	essential oils of parsley, savory and rosemary as prebiotics	1962:2021	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	0	29	theme	Gut	68:70	arg1	Microbiota					72:81	Gut Microbiota	68:81	Gut Microbiota	68:81	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	10	30	theme	essential	1696:1704	arg1	oil					1706:1708	savory essential oil	1689:1708	savory essential oil	1689:1708	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	4	31	dep	Satureja	651:658	arg1	hortensis					660:668	Satureja hortensis	651:668	Satureja hortensis	651:668	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	11	32	theme	protein	1819:1825	arg1	carbonyls					1827:1835	protein carbonyls	1819:1835	protein carbonyls	1819:1835	Finally, there was a decrease in protein carbonyls and pentosidine according to the essential oil emulsion.
36364913	6	33	theme	gut	1016:1018	arg1	composition					1031:1041	gut microbiota composition	1016:1041	gut microbiota composition	1016:1041	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	0	34	theme	Beneficial	0:9	arg1	Effects					11:17	Beneficial Effects	0:17	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus	0:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	4	35	dep	parsley	672:678	arg1	crispum					694:700	Petroselinum crispum	681:700	Petroselinum crispum	681:700	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	8	36	theme	plasma	1356:1361	arg1	TMAO					1363:1366	plasma TMAO	1356:1366	plasma TMAO	1356:1366	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	7	37	theme	commensal	1284:1292	arg1	genus					1325:1329	Lactobacillus genus	1311:1329	Lactobacillus genus	1311:1329	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	7	37	theme	commensal	1284:1292	arg1	bacteria					1294:1301	beneficial commensal bacteria	1273:1301	beneficial commensal bacteria	1273:1301	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	2	38	theme	cardiovascular	424:437	arg1	diseases					453:460	cardiovascular and metabolic diseases	424:460	cardiovascular and metabolic diseases	424:460	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	39	theme	fatty	383:387	arg1	SCFAs					396:400	SCFAs	396:400	SCFAs	396:400	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	39	theme	fatty	383:387	arg1	acids					389:393	short-chain fatty acids	371:393	short-chain fatty acids (SCFAs)	371:401	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	10	40	theme	plasma	1638:1643	arg1	cytokines					1645:1653	plasma cytokines	1638:1653	plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22)	1638:1686	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	5	41	theme	Humanized	805:813	arg1	mice					815:818	Humanized mice	805:818	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM	805:891	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	0	42	theme	Oils	32:35	arg1	Effects					11:17	Beneficial Effects	0:17	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus	0:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	3	43	theme	Mediterranean	596:608	arg1	diet					610:613	the Mediterranean diet	592:613	the Mediterranean diet	592:613	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	10	44	from	decrease	1626:1633	arg1	cytokines					1645:1653	plasma cytokines	1638:1653	plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22)	1638:1686	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	7	45	theme	prebiotic	1252:1260	arg1	effects					1262:1268	prebiotic effects	1252:1268	prebiotic effects	1252:1268	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	8	46	theme	fecal	1387:1391	arg1	SCFAs					1393:1397	fecal SCFAs	1387:1397	fecal SCFAs levels in mice treated with parsley and rosemary essential oils	1387:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	2	47	theme	microbial	306:314	arg1	metabolites					316:326	Gut microbial metabolites	302:326	Gut microbial metabolites	302:326	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	47	theme	microbial	306:314	arg1	acids					389:393	short-chain fatty acids	371:393	short-chain fatty acids (SCFAs)	371:401	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	47	theme	microbial	306:314	arg1	N-oxide					352:358	trimethylamine N-oxide	337:358	trimethylamine N-oxide (TMAO)	337:365	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	1	48	theme	diabetes	201:208	arg1	T2DM					220:223	T2DM	220:223	T2DM	220:223	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	1	48	theme	diabetes	201:208	arg1	mellitus					210:217	type-2 diabetes mellitus	194:217	type-2 diabetes mellitus (T2DM)	194:224	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	6	49	theme	microbial	1058:1066	arg1	metabolites					1068:1078	microbial metabolites	1058:1078	microbial metabolites	1058:1078	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	6	49	theme	microbial	1058:1066	arg1	effects					1005:1011	the effects	1001:1011	the effects on gut microbiota composition and abundance	1001:1055	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	12	50	theme	gut	1936:1938	arg1	microbiota					1940:1949	the gut microbiota	1932:1949	the gut microbiota	1932:1949	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	5	51	theme	oil	962:964	arg1	emulsions					966:974	essential oil emulsions	952:974	essential oil emulsions	952:974	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	3	52	theme	Previous	463:470	arg1	studies					472:478	Previous studies	463:478	Previous studies	463:478	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	7	53	from	emulsions	1199:1207	arg1	mice					1212:1215	mice	1212:1215	mice supplemented with L-carnitine	1212:1245	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	6	54	from	effects	1005:1011	arg1	abundance					1047:1055	abundance	1047:1055	abundance	1047:1055	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	6	54	from	effects	1005:1011	arg1	composition					1031:1041	gut microbiota composition	1016:1041	gut microbiota composition	1016:1041	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	1	55	theme	major	233:237	arg1	problems					246:253	major health problems worldwide	233:263	major health problems worldwide	233:263	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	5	56	attach	derived	845:851	arg2	microbiota					834:843	gut microbiota	830:843	gut microbiota derived from that of patients with IHD and T2DM	830:891	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	5	56	attach	derived	845:851	arg1	that					858:861	that	858:861	that	858:861	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	10	57	theme	savory	1689:1694	arg1	oil					1706:1708	savory essential oil	1689:1708	savory essential oil	1689:1708	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	1	58	theme	Ischemic	161:168	arg1	disease					176:182	Ischemic heart disease	161:182	Ischemic heart disease (IHD)	161:188	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	1	58	theme	Ischemic	161:168	arg1	IHD					185:187	IHD	185:187	IHD	185:187	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	12	59	from	changes	1921:1927	arg1	microbiota					1940:1949	the gut microbiota	1932:1949	the gut microbiota	1932:1949	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	4	60	theme	Essential	616:624	arg1	prebiotics					777:786	prebiotics	777:786	prebiotics	777:786	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	60	theme	Essential	616:624	arg1	emulsions					630:638	Essential oil emulsions	616:638	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis)	616:739	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	60	theme	Essential	616:624	arg1	nutraceuticals					758:771	nutraceuticals	758:771	nutraceuticals	758:771	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	10	61	theme	increased	1730:1738	arg1	levels					1740:1745	increased levels	1730:1745	increased levels of chemokines (CXCL1, CCL2 and CCL11)	1730:1783	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	12	62	from	risk	2163:2166	arg1	patients					2171:2178	patients	2171:2178	patients affected by T2DM	2171:2195	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	1	63	dep	problems	246:253	arg1	worldwide					255:263	worldwide	255:263	major health problems worldwide	233:263	Ischemic heart disease (IHD) and type-2 diabetes mellitus (T2DM) remain major health problems worldwide and commonly coexist in individuals.
36364913	6	64	theme	cardiovascular	1102:1115	arg1	disease					1117:1123	cardiovascular disease	1102:1123	cardiovascular disease	1102:1123	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	0	65	theme	Heart	117:121	arg1	Disease					123:129	Ischemic Heart Disease	108:129	Ischemic Heart Disease	108:129	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	0	66	from	Microbiota	72:81	arg1	Disease					123:129	Ischemic Heart Disease	108:129	Ischemic Heart Disease	108:129	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	0	66	from	Microbiota	72:81	arg1	Mellitus					151:158	Type-2 Diabetes Mellitus	135:158	Type-2 Diabetes Mellitus	135:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	10	67	theme	essential	1602:1610	arg1	oils					1612:1615	rosemary essential oils	1593:1615	rosemary essential oils	1593:1615	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	3	68	theme	prebiotic	552:560	arg1	effects					562:568	the prebiotic effects	548:568	the prebiotic effects of some components of the Mediterranean diet	548:613	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	10	69	dep	chemokines	1750:1759	arg1	chemokines					1750:1759	chemokines	1750:1759	chemokines (CXCL1, CCL2 and CCL11)	1750:1783	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	10	69	dep	chemokines	1750:1759	arg1	CCL11					1778:1782	CCL11	1778:1782	CCL11	1778:1782	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	10	69	dep	chemokines	1750:1759	arg1	CCL2					1769:1772	CCL2	1769:1772	CCL2	1769:1772	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	10	69	dep	chemokines	1750:1759	arg1	CXCL1					1762:1766	CXCL1	1762:1766	CXCL1	1762:1766	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	0	70	theme	Diabetes	142:149	arg1	Mellitus					151:158	Type-2 Diabetes Mellitus	135:158	Type-2 Diabetes Mellitus	135:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	4	71	from	prebiotics	777:786	arg1	T2DM					799:802	T2DM	799:802	T2DM	799:802	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	71	from	prebiotics	777:786	arg1	IHD					791:793	IHD	791:793	IHD	791:793	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	3	72	theme	components	578:587	arg1	effects					562:568	the prebiotic effects	548:568	the prebiotic effects of some components of the Mediterranean diet	548:613	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	3	72	theme	components	578:587	arg1	microbiota					515:524	the gut microbiota	507:524	the gut microbiota of these patients	507:542	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	4	73	from	nutraceuticals	758:771	arg1	T2DM					799:802	T2DM	799:802	T2DM	799:802	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	73	from	nutraceuticals	758:771	arg1	IHD					791:793	IHD	791:793	IHD	791:793	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	9	74	theme	Thrombomodulin	1464:1477	arg1	levels					1479:1484	Thrombomodulin levels	1464:1484	Thrombomodulin levels	1464:1484	Thrombomodulin levels were increased in mice treated with savory and parsley essential oils.
36364913	8	75	theme	essential	1448:1456	arg1	oils					1458:1461	parsley and rosemary essential oils	1427:1461	parsley and rosemary essential oils	1427:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	11	76	from	decrease	1807:1814	arg1	pentosidine					1841:1851	pentosidine	1841:1851	pentosidine	1841:1851	Finally, there was a decrease in protein carbonyls and pentosidine according to the essential oil emulsion.
36364913	11	76	from	decrease	1807:1814	arg1	carbonyls					1827:1835	protein carbonyls	1819:1835	protein carbonyls	1819:1835	Finally, there was a decrease in protein carbonyls and pentosidine according to the essential oil emulsion.
36364913	4	77	theme	parsley	672:678	arg1	prebiotics					777:786	prebiotics	777:786	prebiotics	777:786	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	77	theme	parsley	672:678	arg1	emulsions					630:638	Essential oil emulsions	616:638	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis)	616:739	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	77	theme	parsley	672:678	arg1	nutraceuticals					758:771	nutraceuticals	758:771	nutraceuticals	758:771	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	7	78	theme	essential	1185:1193	arg1	emulsions					1199:1207	essential oil emulsions	1185:1207	essential oil emulsions in mice supplemented with L-carnitine	1185:1245	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	4	79	theme	savory	643:648	arg1	prebiotics					777:786	prebiotics	777:786	prebiotics	777:786	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	79	theme	savory	643:648	arg1	emulsions					630:638	Essential oil emulsions	616:638	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis)	616:739	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	79	theme	savory	643:648	arg1	nutraceuticals					758:771	nutraceuticals	758:771	nutraceuticals	758:771	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	0	80	from	Effects	11:17	arg1	Diet					60:63	the Mediterranean Diet	42:63	the Mediterranean Diet	42:63	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	0	80	from	Effects	11:17	arg1	Microbiota					72:81	Gut Microbiota	68:81	Gut Microbiota	68:81	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	0	80	from	Effects	11:17	arg1	Metabolites					93:103	Their Metabolites	87:103	Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus	87:158	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	4	81	theme	rosemary	707:714	arg1	prebiotics					777:786	prebiotics	777:786	prebiotics	777:786	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	81	theme	rosemary	707:714	arg1	emulsions					630:638	Essential oil emulsions	616:638	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis)	616:739	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	81	theme	rosemary	707:714	arg1	nutraceuticals					758:771	nutraceuticals	758:771	nutraceuticals	758:771	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	2	82	attach	linked	414:419	arg1	diseases					453:460	cardiovascular and metabolic diseases	424:460	cardiovascular and metabolic diseases	424:460	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	82	attach	linked	414:419	arg2	N-oxide					352:358	trimethylamine N-oxide	337:358	trimethylamine N-oxide (TMAO)	337:365	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	82	attach	linked	414:419	arg2	acids					389:393	short-chain fatty acids	371:393	short-chain fatty acids (SCFAs)	371:401	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	82	attach	linked	414:419	arg2	metabolites					316:326	Gut microbial metabolites	302:326	Gut microbial metabolites	302:326	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	9	83	theme	savory	1522:1527	arg1	oils					1551:1554	savory and parsley essential oils	1522:1554	savory and parsley essential oils	1522:1554	Thrombomodulin levels were increased in mice treated with savory and parsley essential oils.
36364913	6	84	theme	microbiota	1020:1029	arg1	composition					1031:1041	gut microbiota composition	1016:1041	gut microbiota composition	1016:1041	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	4	85	theme	Rosmarinus	717:726	arg1	rosemary					707:714	rosemary	707:714	rosemary (Rosmarinus officinalis)	707:739	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	4	85	theme	Rosmarinus	717:726	arg1	officinalis					728:738	Rosmarinus officinalis	717:738	Rosmarinus officinalis	717:738	Essential oil emulsions of savory (Satureja hortensis), parsley (Petroselinum crispum) and rosemary (Rosmarinus officinalis) were assessed as nutraceuticals and prebiotics in IHD and T2DM.
36364913	7	86	contain	have	1247:1250	arg2	effects					1262:1268	prebiotic effects	1252:1268	prebiotic effects	1252:1268	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	7	86	contain	have	1247:1250	arg1	emulsions					1199:1207	essential oil emulsions	1185:1207	essential oil emulsions in mice supplemented with L-carnitine	1185:1245	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	9	87	theme	parsley	1533:1539	arg1	oils					1551:1554	savory and parsley essential oils	1522:1554	savory and parsley essential oils	1522:1554	Thrombomodulin levels were increased in mice treated with savory and parsley essential oils.
36364913	5	88	with	patients	866:873	arg1	T2DM					888:891	T2DM	888:891	T2DM	888:891	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	5	88	with	patients	866:873	arg1	IHD					880:882	IHD	880:882	IHD	880:882	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	12	89	theme	nutraceuticals	2131:2144	arg1	potential					2112:2120	the potential	2108:2120	the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM	2108:2195	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	0	90	theme	Essential	22:30	arg1	Oils					32:35	Essential Oils	22:35	Essential Oils from the Mediterranean Diet	22:63	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	11	91	theme	oil	1880:1882	arg1	emulsion					1884:1891	the essential oil emulsion	1866:1891	the essential oil emulsion	1866:1891	Finally, there was a decrease in protein carbonyls and pentosidine according to the essential oil emulsion.
36364913	7	92	theme	beneficial	1273:1282	arg1	genus					1325:1329	Lactobacillus genus	1311:1329	Lactobacillus genus	1311:1329	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	7	92	theme	beneficial	1273:1282	arg1	bacteria					1294:1301	beneficial commensal bacteria	1273:1301	beneficial commensal bacteria	1273:1301	Our results showed that essential oil emulsions in mice supplemented with L-carnitine have prebiotic effects on beneficial commensal bacteria, mainly Lactobacillus genus.
36364913	3	93	theme	diet	610:613	arg1	components					578:587	some components	573:587	some components of the Mediterranean diet	573:613	Previous studies have reported dysbiosis in the gut microbiota of these patients and the prebiotic effects of some components of the Mediterranean diet.
36364913	10	94	dep	TNFα	1662:1665	arg1	IL-22					1681:1685	IL-22	1681:1685	IL-22	1681:1685	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	10	94	dep	TNFα	1662:1665	arg1	IL-12p70					1668:1675	IL-12p70	1668:1675	IL-12p70	1668:1675	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	12	95	theme	essential	1962:1970	arg1	oils					1972:1975	essential oils	1962:1975	essential oils of parsley, savory and rosemary as prebiotics	1962:2021	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	2	96	theme	short-chain	371:381	arg1	SCFAs					396:400	SCFAs	396:400	SCFAs	396:400	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	96	theme	short-chain	371:381	arg1	acids					389:393	short-chain fatty acids	371:393	short-chain fatty acids (SCFAs)	371:401	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	0	97	theme	Mediterranean	46:58	arg1	Diet					60:63	the Mediterranean Diet	42:63	the Mediterranean Diet	42:63	Beneficial Effects of Essential Oils from the Mediterranean Diet on Gut Microbiota and Their Metabolites in Ischemic Heart Disease and Type-2 Diabetes Mellitus.
36364913	6	98	theme	plasma	1084:1089	arg1	markers					1091:1097	plasma markers	1084:1097	plasma markers	1084:1097	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	6	98	theme	plasma	1084:1089	arg1	effects					1005:1011	the effects	1001:1011	the effects on gut microbiota composition and abundance	1001:1055	We assessed the effects on gut microbiota composition and abundance, microbial metabolites and plasma markers of cardiovascular disease, inflammation and oxidative stress.
36364913	10	99	dep	cytokines	1645:1653	arg1	TNFα					1662:1665	TNFα	1662:1665	TNFα	1662:1665	While mice treated with parsley and rosemary essential oils showed a decrease in plasma cytokines (INFɣ, TNFα, IL-12p70 and IL-22); savory essential oil was associated with increased levels of chemokines (CXCL1, CCL2 and CCL11).
36364913	2	100	theme	Gut	302:304	arg1	metabolites					316:326	Gut microbial metabolites	302:326	Gut microbial metabolites	302:326	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	100	theme	Gut	302:304	arg1	acids					389:393	short-chain fatty acids	371:393	short-chain fatty acids (SCFAs)	371:401	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	100	theme	Gut	302:304	arg1	N-oxide					352:358	trimethylamine N-oxide	337:358	trimethylamine N-oxide (TMAO)	337:365	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	12	101	theme	cardiovascular	2053:2066	arg1	factors					2082:2088	cardiovascular and metabolic factors	2053:2088	cardiovascular and metabolic factors	2053:2088	These results suggest that changes in the gut microbiota induced by essential oils of parsley, savory and rosemary as prebiotics could differentially regulate cardiovascular and metabolic factors, which highlights the potential of these nutraceuticals for reducing IHD risk in patients affected by T2DM.
36364913	5	102	theme	essential	952:960	arg1	emulsions					966:974	essential oil emulsions	952:974	essential oil emulsions	952:974	Humanized mice harboring gut microbiota derived from that of patients with IHD and T2DM were supplemented with L-carnitine and orally treated with essential oil emulsions for 40 days.
36364913	8	103	theme	SCFAs	1393:1397	arg1	levels					1399:1404	fecal SCFAs levels	1387:1404	fecal SCFAs levels in mice treated with parsley and rosemary essential oils	1387:1461	There was a decrease in plasma TMAO and an increase in fecal SCFAs levels in mice treated with parsley and rosemary essential oils.
36364913	2	104	theme	trimethylamine	337:350	arg1	TMAO					361:364	TMAO	361:364	TMAO	361:364	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36364913	2	104	theme	trimethylamine	337:350	arg1	N-oxide					352:358	trimethylamine N-oxide	337:358	trimethylamine N-oxide (TMAO)	337:365	Gut microbial metabolites, such as trimethylamine N-oxide (TMAO) and short-chain fatty acids (SCFAs), have been linked to cardiovascular and metabolic diseases.
36361655	6	0	theme	cytotoxicity	1004:1015	arg1	data					1017:1020	cytotoxicity data	1004:1020	cytotoxicity data obtained from MTT assays	1004:1045	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	9	1	theme	toxicity	1588:1595	arg1	evaluation					1597:1606	Preliminary toxicity evaluation	1576:1606	Preliminary toxicity evaluation	1576:1606	Preliminary toxicity evaluation also indicated that its composition can be safely used in humans for cancer therapy.
36361655	6	2	from	decrease	1090:1097	arg1	viability					1114:1122	cancer cell viability	1102:1122	cancer cell viability	1102:1122	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	1	3	theme	turmeric	156:163	arg1	root					165:168	turmeric root	156:168	turmeric root	156:168	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	7	4	theme	cancer	1382:1387	arg1	cells					1413:1417	SW480 and HCT116 cells	1396:1417	cells	1413:1417	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	7	4	theme	cancer	1382:1387	arg1	cells					1389:1393	colorectal cancer cells	1371:1393	colorectal cancer cells (SW480 and HCT116 cells)	1371:1418	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	0	5	theme	Cancer	95:100	arg1	Cells					102:106	Colorectal Cancer Cells	84:106	Colorectal Cancer Cells	84:106	Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells.
36361655	2	6	theme	water	348:352	arg1	solubility					354:363	low water solubility	344:363	low water solubility	344:363	However, the use of this efficacious agent in cancer therapy has been limited due to low water solubility and poor bioavailability.
36361655	8	7	theme	chemotherapeutic	1545:1560	arg1	applications					1562:1573	various chemotherapeutic applications	1537:1573	various chemotherapeutic applications	1537:1573	This efficacious formulation that enables improved aqueous dispersion is potentially useful and can be extended for various chemotherapeutic applications.
36361655	6	8	theme	greater	1082:1088	arg1	decrease					1090:1097	a greater decrease	1080:1097	a greater decrease in cancer cell viability	1080:1122	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	7	9	theme	SW480	1396:1400	arg1	cells					1413:1417	SW480 and HCT116 cells	1396:1417	cells	1413:1417	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	7	9	theme	SW480	1396:1400	arg1	cells					1389:1393	colorectal cancer cells	1371:1393	colorectal cancer cells (SW480 and HCT116 cells)	1371:1418	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	4	10	theme	dissolution	759:769	arg1	assays					771:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	11	theme	spectroscopy	605:616	arg1	assays					771:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	8	12	theme	various	1537:1543	arg1	applications					1562:1573	various chemotherapeutic applications	1537:1573	various chemotherapeutic applications	1537:1573	This efficacious formulation that enables improved aqueous dispersion is potentially useful and can be extended for various chemotherapeutic applications.
36361655	0	13	from	β-Cyclodextrins	65:79	arg1	Cells					102:106	Colorectal Cancer Cells	84:106	Colorectal Cancer Cells	84:106	Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells.
36361655	2	14	theme	agent	296:300	arg1	use					272:274	the use	268:274	the use of this efficacious agent in cancer therapy	268:318	However, the use of this efficacious agent in cancer therapy has been limited due to low water solubility and poor bioavailability.
36361655	3	15	theme	anticancer	551:560	arg1	effects					562:568	enhanced in vitro anticancer effects	533:568	enhanced in vitro anticancer effects	533:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	4	16	theme	microscopy	726:735	arg1	assays					771:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	7	17	theme	migration	1168:1176	arg1	rates					1178:1182	cancer cell migration rates	1156:1182	cancer cell migration rates	1156:1182	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	4	18	theme	electron	717:724	arg1	SEM					738:740	SEM	738:740	SEM	738:740	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	18	theme	electron	717:724	arg1	microscopy					726:735	scanning electron microscopy	708:735	scanning electron microscopy (SEM)	708:741	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	2	19	theme	efficacious	284:294	arg1	agent					296:300	this efficacious agent	279:300	this efficacious agent	279:300	However, the use of this efficacious agent in cancer therapy has been limited due to low water solubility and poor bioavailability.
36361655	3	20	theme	drug	421:424	arg1	system					435:440	a drug delivery system	419:440	a drug delivery system	419:440	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	3	20	theme	drug	421:424	arg1	excipient					464:472	an excipient	461:472	an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects	461:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	4	21	theme	UV-vis	598:603	arg1	spectroscopy					605:616	UV-vis spectroscopy	598:616	UV-vis spectroscopy	598:616	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	7	22	theme	HCT116	1406:1411	arg1	cells					1413:1417	SW480 and HCT116 cells	1396:1417	cells	1413:1417	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	7	22	theme	HCT116	1406:1411	arg1	cells					1389:1393	colorectal cancer cells	1371:1393	colorectal cancer cells (SW480 and HCT116 cells)	1371:1418	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	3	23	theme	delivery	426:433	arg1	system					435:440	a drug delivery system	419:440	a drug delivery system	419:440	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	3	23	theme	delivery	426:433	arg1	excipient					464:472	an excipient	461:472	an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects	461:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	3	24	theme	in	542:543	arg1	effects					562:568	enhanced in vitro anticancer effects	533:568	enhanced in vitro anticancer effects	533:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	7	25	theme	colorectal	1371:1380	arg1	cells					1413:1417	SW480 and HCT116 cells	1396:1417	cells	1413:1417	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	7	25	theme	colorectal	1371:1380	arg1	cells					1389:1393	colorectal cancer cells	1371:1393	colorectal cancer cells (SW480 and HCT116 cells)	1371:1418	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	4	26	theme	Fourier	660:666	arg1	assays					771:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	7	27	theme	cancer	1156:1161	arg1	rates					1178:1182	cancer cell migration rates	1156:1182	cancer cell migration rates	1156:1182	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	4	28	theme	Different	571:579	arg1	assays					771:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	28	theme	Different	571:579	arg1	analyses					581:588	Different analyses	571:588	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	571:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	5	29	theme	water	928:932	arg1	solubility					934:943	water solubility	928:943	water solubility	928:943	The results indicated that water solubility was improved by 205.75-fold compared to pure CUR.
36361655	6	30	theme	inclusion	1052:1060	arg1	complex					1062:1068	the inclusion complex	1048:1068	the inclusion complex	1048:1068	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	1	31	theme	diverse	181:187	arg1	properties					247:256	potent anticancer properties	229:256	potent anticancer properties	229:256	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	1	31	theme	diverse	181:187	arg1	applications					205:216	diverse pharmacological applications	181:216	diverse pharmacological applications	181:216	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	0	32	theme	Biophysical	0:10	arg1	Evaluation					12:21	Biophysical Evaluation	0:21	Biophysical Evaluation of Water-Soluble Curcumin	0:47	Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells.
36361655	8	33	theme	aqueous	1472:1478	arg1	dispersion					1480:1489	improved aqueous dispersion	1463:1489	improved aqueous dispersion	1463:1489	This efficacious formulation that enables improved aqueous dispersion is potentially useful and can be extended for various chemotherapeutic applications.
36361655	4	34	theme	inner	852:856	arg1	cavity					858:863	the inner cavity	848:863	the inner cavity of a β-cyclodextrin (β-CD) complex	848:898	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	3	35	from	dispersion	492:501	arg1	media					514:518	aqueous media	506:518	aqueous media coupled with enhanced in vitro anticancer effects	506:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	1	36	theme	pharmacological	189:203	arg1	properties					247:256	potent anticancer properties	229:256	potent anticancer properties	229:256	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	1	36	theme	pharmacological	189:203	arg1	applications					205:216	diverse pharmacological applications	181:216	diverse pharmacological applications	181:216	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	0	37	theme	Water-Soluble	26:38	arg1	Curcumin					40:47	Water-Soluble Curcumin	26:47	Water-Soluble Curcumin	26:47	Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells.
36361655	8	38	theme	improved	1463:1470	arg1	dispersion					1480:1489	improved aqueous dispersion	1463:1489	improved aqueous dispersion	1463:1489	This efficacious formulation that enables improved aqueous dispersion is potentially useful and can be extended for various chemotherapeutic applications.
36361655	4	39	theme	solubility	744:753	arg1	assays					771:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	6	40	theme	cancer	1102:1107	arg1	viability					1114:1122	cancer cell viability	1102:1122	cancer cell viability	1102:1122	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	8	41	theme	efficacious	1426:1436	arg1	formulation					1438:1448	This efficacious formulation	1421:1448	This efficacious formulation that enables improved aqueous dispersion	1421:1489	This efficacious formulation that enables improved aqueous dispersion is potentially useful and can be extended for various chemotherapeutic applications.
36361655	3	42	theme	enhanced	533:540	arg1	effects					562:568	enhanced in vitro anticancer effects	533:568	enhanced in vitro anticancer effects	533:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	2	43	theme	cancer	305:310	arg1	therapy					312:318	cancer therapy	305:318	cancer therapy	305:318	However, the use of this efficacious agent in cancer therapy has been limited due to low water solubility and poor bioavailability.
36361655	3	44	theme	aqueous	506:512	arg1	media					514:518	aqueous media	506:518	aqueous media coupled with enhanced in vitro anticancer effects	506:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	4	45	theme	scanning	708:715	arg1	SEM					738:740	SEM	738:740	SEM	738:740	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	45	theme	scanning	708:715	arg1	microscopy					726:735	scanning electron microscopy	708:735	scanning electron microscopy (SEM)	708:741	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	7	46	theme	cell	1163:1166	arg1	rates					1178:1182	cancer cell migration rates	1156:1182	cancer cell migration rates	1156:1182	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	2	47	from	use	272:274	arg1	therapy					312:318	cancer therapy	305:318	cancer therapy	305:318	However, the use of this efficacious agent in cancer therapy has been limited due to low water solubility and poor bioavailability.
36361655	6	48	theme	MTT	1036:1038	arg1	assays					1040:1045	MTT assays	1036:1045	MTT assays	1036:1045	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	0	49	theme	Curcumin	40:47	arg1	Evaluation					12:21	Biophysical Evaluation	0:21	Biophysical Evaluation of Water-Soluble Curcumin	0:47	Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells.
36361655	1	50	contain	possesses	171:179	arg1	Curcumin					109:116	Curcumin	109:116	Curcumin (CUR)	109:122	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	1	50	contain	possesses	171:179	arg1	curcuminoid					127:137	a curcuminoid	125:137	a curcuminoid originating from turmeric root	125:168	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	1	50	contain	possesses	171:179	arg2	applications					205:216	diverse pharmacological applications	181:216	diverse pharmacological applications	181:216	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	1	50	contain	possesses	171:179	arg2	properties					247:256	potent anticancer properties	229:256	potent anticancer properties	229:256	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	1	50	contain	possesses	171:179	arg1	CUR					119:121	CUR	119:121	CUR	119:121	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	4	51	theme	successful	809:818	arg1	encapsulation					820:832	the successful encapsulation	805:832	the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex	805:898	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	1	52	theme	potent	229:234	arg1	properties					247:256	potent anticancer properties	229:256	potent anticancer properties	229:256	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	4	53	theme	CUR	837:839	arg1	encapsulation					820:832	the successful encapsulation	805:832	the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex	805:898	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	9	54	theme	cancer	1677:1682	arg1	therapy					1684:1690	cancer therapy	1677:1690	cancer therapy	1677:1690	Preliminary toxicity evaluation also indicated that its composition can be safely used in humans for cancer therapy.
36361655	2	55	theme	low	344:346	arg1	solubility					354:363	low water solubility	344:363	low water solubility	344:363	However, the use of this efficacious agent in cancer therapy has been limited due to low water solubility and poor bioavailability.
36361655	4	56	theme	β-CD	886:889	arg1	complex					892:898	a β-cyclodextrin (β-CD) complex	868:898	a β-cyclodextrin (β-CD) complex	868:898	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	9	57	used	used	1658:1661	arg2	composition					1632:1642	its composition	1628:1642	its composition	1628:1642	Preliminary toxicity evaluation also indicated that its composition can be safely used in humans for cancer therapy.
36361655	3	58	dep	in	542:543	arg1	vitro					545:549	vitro	545:549	vitro	545:549	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	7	59	theme	invasion	1220:1227	arg1	rates					1229:1233	invasion rates	1220:1233	invasion rates	1220:1233	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	7	60	theme	caspase	1343:1349	arg1	activation					1353:1362	caspase 3 activation	1343:1362	caspase 3 activation	1343:1362	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	4	61	theme	infrared	678:685	arg1	FTIR					701:704	FTIR	701:704	FTIR	701:704	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	61	theme	infrared	678:685	arg1	spectroscopy					687:698	infrared spectroscopy	678:698	infrared spectroscopy (FTIR)	678:705	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	5	62	theme	pure	985:988	arg1	CUR					990:992	pure CUR	985:992	pure CUR	985:992	The results indicated that water solubility was improved by 205.75-fold compared to pure CUR.
36361655	9	63	theme	Preliminary	1576:1586	arg1	evaluation					1597:1606	Preliminary toxicity evaluation	1576:1606	Preliminary toxicity evaluation	1576:1606	Preliminary toxicity evaluation also indicated that its composition can be safely used in humans for cancer therapy.
36361655	6	64	theme	cell	1109:1112	arg1	viability					1114:1122	cancer cell viability	1102:1122	cancer cell viability	1102:1122	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	6	65	theme	pure	1136:1139	arg1	CUR					1141:1143	pure CUR	1136:1143	pure CUR	1136:1143	Based on cytotoxicity data obtained from MTT assays, the inclusion complex exhibited a greater decrease in cancer cell viability compared to pure CUR.
36361655	1	66	theme	anticancer	236:245	arg1	properties					247:256	potent anticancer properties	229:256	potent anticancer properties	229:256	Curcumin (CUR), a curcuminoid originating from turmeric root, possesses diverse pharmacological applications, including potent anticancer properties.
36361655	4	67	theme	complex	892:898	arg1	cavity					858:863	the inner cavity	848:863	the inner cavity of a β-cyclodextrin (β-CD) complex	848:898	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	3	68	theme	improved	483:490	arg1	dispersion					492:501	improved dispersion	483:501	improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects	483:568	To overcome these problems, a drug delivery system was established as an excipient allowing improved dispersion in aqueous media coupled with enhanced in vitro anticancer effects.
36361655	4	69	theme	β-cyclodextrin	870:883	arg1	complex					892:898	a β-cyclodextrin (β-CD) complex	868:898	a β-cyclodextrin (β-CD) complex	868:898	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	0	70	theme	Colorectal	84:93	arg1	Cells					102:106	Colorectal Cancer Cells	84:106	Colorectal Cancer Cells	84:106	Biophysical Evaluation of Water-Soluble Curcumin Encapsulated in β-Cyclodextrins on Colorectal Cancer Cells.
36361655	2	71	theme	poor	369:372	arg1	bioavailability					374:388	poor bioavailability	369:388	poor bioavailability	369:388	However, the use of this efficacious agent in cancer therapy has been limited due to low water solubility and poor bioavailability.
36361655	7	72	theme	apoptosis	1276:1284	arg1	rates					1286:1290	apoptosis rates	1276:1290	apoptosis rates	1276:1290	Moreover, cancer cell migration rates were decreased by 75.5% and 38.92%, invasion rates were decreased by 37.7% and 35.7%, while apoptosis rates were increased by 26.3% and 14.2%, and both caused caspase 3 activation toward colorectal cancer cells (SW480 and HCT116 cells).
36361655	4	73	theme	calorimetry	641:651	arg1	assays					771:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays	598:776	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	74	dep	Fourier	660:666	arg1	transform					668:676	transform	668:676	transform infrared spectroscopy (FTIR)	668:705	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	75	theme	differential	619:630	arg1	DSC					654:656	DSC	654:656	DSC	654:656	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	75	theme	differential	619:630	arg1	calorimetry					641:651	differential scanning calorimetry	619:651	differential scanning calorimetry (DSC)	619:657	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	76	theme	scanning	632:639	arg1	DSC					654:656	DSC	654:656	DSC	654:656	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36361655	4	76	theme	scanning	632:639	arg1	calorimetry					641:651	differential scanning calorimetry	619:651	differential scanning calorimetry (DSC)	619:657	Different analyses such as UV-vis spectroscopy, differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), solubility and dissolution assays were determined to monitor the successful encapsulation of CUR within the inner cavity of a β-cyclodextrin (β-CD) complex.
36144531	4	0	theme	phenolic	700:707	arg1	content					718:724	high phenolic compound content	695:724	high phenolic compound content	695:724	This study demonstrated that high phenolic compound content and antioxidant capacity were obtained from amaranth grain using ethanol as the extraction solvent.
36144531	3	1	theme	different	514:522	arg1	permeability					561:572	water vapor permeability	549:572	water vapor permeability	549:572	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	1	theme	different	514:522	arg1	properties					531:540	different film's properties	514:540	different film's properties	514:540	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	7	2	theme	phenolic	1358:1365	arg1	compounds					1367:1375	amaranth phenolic compounds	1349:1375	amaranth phenolic compounds	1349:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	2	3	from	concentrations	355:368	arg1	compounds					334:342	Alginate, glycerol, and amaranth grain phenolic compounds	286:342	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations	286:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	4	4	theme	high	695:698	arg1	content					718:724	high phenolic compound content	695:724	high phenolic compound content	695:724	This study demonstrated that high phenolic compound content and antioxidant capacity were obtained from amaranth grain using ethanol as the extraction solvent.
36144531	7	5	used	used	1399:1402	arg2	films					1338:1342	active alginate films	1322:1342	active alginate films with amaranth phenolic compounds	1322:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	7	5	used	used	1399:1402	arg2	material					1422:1429	food packaging material	1407:1429	food packaging material with potential antioxidant activity	1407:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	0	6	theme	Alginate-Based	85:98	arg1	Films					107:111	Alginate-Based Active Films	85:111	Alginate-Based Active Films	85:111	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	2	7	theme	Alginate	286:293	arg1	compounds					334:342	Alginate, glycerol, and amaranth grain phenolic compounds	286:342	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations	286:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	2	8	theme	amaranth	310:317	arg1	compounds					334:342	Alginate, glycerol, and amaranth grain phenolic compounds	286:342	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations	286:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	3	9	dep	properties	531:540	arg1	i.e.					543:546	different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties	514:663	i.e.	543:546	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	10	theme	Composite	426:434	arg1	CCRD					454:457	CCRD	454:457	CCRD	454:457	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	10	theme	Composite	426:434	arg1	Design					446:451	An experimental Central Composite Rotatable Design	402:451	An experimental Central Composite Rotatable Design (CCRD)	402:458	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	7	11	theme	food	1407:1410	arg1	films					1338:1342	active alginate films	1322:1342	active alginate films with amaranth phenolic compounds	1322:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	7	11	theme	food	1407:1410	arg1	material					1422:1429	food packaging material	1407:1429	food packaging material with potential antioxidant activity	1407:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	4	12	theme	amaranth	770:777	arg1	grain					779:783	amaranth grain	770:783	amaranth grain using ethanol as the extraction solvent	770:823	This study demonstrated that high phenolic compound content and antioxidant capacity were obtained from amaranth grain using ethanol as the extraction solvent.
36144531	6	13	theme	Component	1232:1240	arg1	PCA					1252:1254	PCA	1252:1254	PCA	1252:1254	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	6	13	theme	Component	1232:1240	arg1	Analysis					1242:1249	a Principal Component Analysis	1220:1249	a Principal Component Analysis (PCA)	1220:1255	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	3	14	theme	water	549:553	arg1	content					600:606	moisture content	591:606	moisture content	591:606	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	14	theme	water	549:553	arg1	properties					531:540	different film's properties	514:540	different film's properties	514:540	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	14	theme	water	549:553	arg1	permeability					561:572	water vapor permeability	549:572	water vapor permeability	549:572	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	14	theme	water	549:553	arg1	solubility					609:618	solubility	609:618	solubility	609:618	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	14	theme	water	549:553	arg1	hydrophobicity					575:588	hydrophobicity	575:588	hydrophobicity	575:588	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	14	theme	water	549:553	arg1	properties					654:663	thermal, mechanical, and optical properties	621:663	thermal, mechanical, and optical properties	621:663	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	0	15	theme	Films	107:111	arg1	Development					70:80	the Development	66:80	the Development of Alginate-Based Active Films	66:111	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	6	16	theme	water-soluble	1119:1131	arg1	films					1133:1137	less flexible but more resistant and water-soluble films	1082:1137	less flexible but more resistant and water-soluble films	1082:1137	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	6	17	theme	resistant	1105:1113	arg1	films					1133:1137	less flexible but more resistant and water-soluble films	1082:1137	less flexible but more resistant and water-soluble films	1082:1137	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	3	18	used	used	464:467	arg2	CCRD					454:457	CCRD	454:457	CCRD	454:457	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	18	used	used	464:467	arg2	Design					446:451	An experimental Central Composite Rotatable Design	402:451	An experimental Central Composite Rotatable Design (CCRD)	402:458	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	7	19	theme	amaranth	1349:1356	arg1	compounds					1367:1375	amaranth phenolic compounds	1349:1375	amaranth phenolic compounds	1349:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	1	20	theme	alginate-based	165:178	arg1	films					180:184	alginate-based films	165:184	alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents	165:283	This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents.
36144531	0	21	theme	Active	100:105	arg1	Films					107:111	Alginate-Based Active Films	85:111	Alginate-Based Active Films	85:111	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	6	22	theme	Squares	1275:1281	arg1	analysis					1289:1296	Partial Least Squares (PLS) analysis	1261:1296	Partial Least Squares (PLS) analysis	1261:1296	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	6	23	theme	alginate	1173:1180	arg1	concentration					1182:1194	the alginate concentration	1169:1194	the alginate concentration	1169:1194	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	2	24	theme	glycerol	296:303	arg1	compounds					334:342	Alginate, glycerol, and amaranth grain phenolic compounds	286:342	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations	286:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	7	25	with	material	1422:1429	arg1	activity					1458:1465	potential antioxidant activity	1436:1465	potential antioxidant activity	1436:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	1	26	theme	films	180:184	arg1	development					150:160	the development	146:160	the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents	146:283	This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents.
36144531	3	27	theme	experimental	405:416	arg1	CCRD					454:457	CCRD	454:457	CCRD	454:457	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	27	theme	experimental	405:416	arg1	Design					446:451	An experimental Central Composite Rotatable Design	402:451	An experimental Central Composite Rotatable Design (CCRD)	402:458	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	6	28	theme	Least	1269:1273	arg1	Squares					1275:1281	Partial Least Squares	1261:1281	Partial Least Squares (PLS) analysis	1261:1296	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	6	28	theme	Least	1269:1273	arg1	PLS					1284:1286	PLS	1284:1286	PLS	1284:1286	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	5	29	theme	alginate	971:978	arg1	films					980:984	alginate films	971:984	alginate films containing amaranth phenolic compounds	971:1023	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	5	30	contain	containing	986:995	arg2	compounds					1015:1023	amaranth phenolic compounds	997:1023	amaranth phenolic compounds	997:1023	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	5	30	contain	containing	986:995	arg1	films					980:984	alginate films	971:984	alginate films containing amaranth phenolic compounds	971:1023	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	7	31	theme	active	1322:1327	arg1	films					1338:1342	active alginate films	1322:1342	active alginate films with amaranth phenolic compounds	1322:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	7	31	theme	active	1322:1327	arg1	material					1422:1429	food packaging material	1407:1429	food packaging material with potential antioxidant activity	1407:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	3	32	theme	Central	418:424	arg1	CCRD					454:457	CCRD	454:457	CCRD	454:457	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	32	theme	Central	418:424	arg1	Design					446:451	An experimental Central Composite Rotatable Design	402:451	An experimental Central Composite Rotatable Design (CCRD)	402:458	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	7	33	theme	potential	1436:1444	arg1	activity					1458:1465	potential antioxidant activity	1436:1465	potential antioxidant activity	1436:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	6	34	theme	Partial	1261:1267	arg1	Squares					1275:1281	Partial Least Squares	1261:1281	Partial Least Squares (PLS) analysis	1261:1296	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	6	34	theme	Partial	1261:1267	arg1	PLS					1284:1286	PLS	1284:1286	PLS	1284:1286	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	7	35	theme	packaging	1412:1420	arg1	films					1338:1342	active alginate films	1322:1342	active alginate films with amaranth phenolic compounds	1322:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	7	35	theme	packaging	1412:1420	arg1	material					1422:1429	food packaging material	1407:1429	food packaging material with potential antioxidant activity	1407:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	1	36	theme	phenolic	200:207	arg1	compounds					209:217	phenolic compounds	200:217	phenolic compounds extracted from Amaranthus cruentus grain using different solvents	200:283	This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents.
36144531	5	37	theme	Alginate	826:833	arg1	films					835:839	Alginate films	826:839	Alginate films incorporating amaranth phenolic compounds	826:881	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	2	38	used	used	375:378	arg2	compounds					334:342	Alginate, glycerol, and amaranth grain phenolic compounds	286:342	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations	286:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	2	38	used	used	375:378	arg2	concentrations					355:368	various concentrations	347:368	various concentrations	347:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	2	39	theme	phenolic	325:332	arg1	compounds					334:342	Alginate, glycerol, and amaranth grain phenolic compounds	286:342	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations	286:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	5	40	theme	amaranth	855:862	arg1	compounds					873:881	amaranth phenolic compounds	855:881	amaranth phenolic compounds	855:881	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	0	41	theme	Amaranth	16:23	arg1	Extracts					53:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	5	42	theme	phenolic	1006:1013	arg1	compounds					1015:1023	amaranth phenolic compounds	997:1023	amaranth phenolic compounds	997:1023	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	2	43	theme	grain	319:323	arg1	compounds					334:342	Alginate, glycerol, and amaranth grain phenolic compounds	286:342	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations	286:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	5	44	theme	phenolic	864:871	arg1	compounds					873:881	amaranth phenolic compounds	855:881	amaranth phenolic compounds	855:881	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	0	45	theme	cruentus	37:44	arg1	Extracts					53:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	3	46	theme	optical	646:652	arg1	permeability					561:572	water vapor permeability	549:572	water vapor permeability	549:572	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	46	theme	optical	646:652	arg1	properties					654:663	thermal, mechanical, and optical properties	621:663	thermal, mechanical, and optical properties	621:663	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	5	47	theme	final	1045:1049	arg1	application					1056:1066	their final food application	1039:1066	their final food application	1039:1066	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	6	48	theme	flexible	1087:1094	arg1	films					1133:1137	less flexible but more resistant and water-soluble films	1082:1137	less flexible but more resistant and water-soluble films	1082:1137	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	7	49	theme	antioxidant	1446:1456	arg1	activity					1458:1465	potential antioxidant activity	1436:1465	potential antioxidant activity	1436:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	0	50	theme	Amaranthus	26:35	arg1	Extracts					53:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	3	51	theme	mechanical	630:639	arg1	permeability					561:572	water vapor permeability	549:572	water vapor permeability	549:572	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	51	theme	mechanical	630:639	arg1	properties					654:663	thermal, mechanical, and optical properties	621:663	thermal, mechanical, and optical properties	621:663	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	5	52	theme	food	1051:1054	arg1	application					1056:1066	their final food application	1039:1066	their final food application	1039:1066	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	1	53	theme	Amaranthus	234:243	arg1	grain					254:258	Amaranthus cruentus grain	234:258	Amaranthus cruentus grain using different solvents	234:283	This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents.
36144531	0	54	theme	Grain	47:51	arg1	Extracts					53:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Amaranth (Amaranthus cruentus) Grain Extracts	16:60	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	5	55	theme	amaranth	997:1004	arg1	compounds					1015:1023	amaranth phenolic compounds	997:1023	amaranth phenolic compounds	997:1023	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	3	56	theme	variables	501:509	arg1	effect					485:490	the effect	481:490	the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties	481:663	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	57	theme	Rotatable	436:444	arg1	CCRD					454:457	CCRD	454:457	CCRD	454:457	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	57	theme	Rotatable	436:444	arg1	Design					446:451	An experimental Central Composite Rotatable Design	402:451	An experimental Central Composite Rotatable Design (CCRD)	402:458	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	7	58	theme	alginate	1329:1336	arg1	films					1338:1342	active alginate films	1322:1342	active alginate films with amaranth phenolic compounds	1322:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	7	58	theme	alginate	1329:1336	arg1	material					1422:1429	food packaging material	1407:1429	food packaging material with potential antioxidant activity	1407:1465	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	3	59	theme	moisture	591:598	arg1	content					600:606	moisture content	591:606	moisture content	591:606	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	59	theme	moisture	591:598	arg1	permeability					561:572	water vapor permeability	549:572	water vapor permeability	549:572	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	4	60	theme	compound	709:716	arg1	content					718:724	high phenolic compound content	695:724	high phenolic compound content	695:724	This study demonstrated that high phenolic compound content and antioxidant capacity were obtained from amaranth grain using ethanol as the extraction solvent.
36144531	5	61	used	used	937:940	arg2	study					924:928	this study	919:928	this study	919:928	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	7	62	with	films	1338:1342	arg1	compounds					1367:1375	amaranth phenolic compounds	1349:1375	amaranth phenolic compounds	1349:1375	This study showed that active alginate films with amaranth phenolic compounds can be tailored to be used as food packaging material with potential antioxidant activity.
36144531	3	63	theme	thermal	621:627	arg1	permeability					561:572	water vapor permeability	549:572	water vapor permeability	549:572	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	63	theme	thermal	621:627	arg1	properties					654:663	thermal, mechanical, and optical properties	621:663	thermal, mechanical, and optical properties	621:663	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	1	64	theme	research	119:126	arg1	work					128:131	This research work	114:131	This research work	114:131	This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents.
36144531	3	65	theme	vapor	555:559	arg1	content					600:606	moisture content	591:606	moisture content	591:606	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	65	theme	vapor	555:559	arg1	properties					531:540	different film's properties	514:540	different film's properties	514:540	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	65	theme	vapor	555:559	arg1	permeability					561:572	water vapor permeability	549:572	water vapor permeability	549:572	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	65	theme	vapor	555:559	arg1	solubility					609:618	solubility	609:618	solubility	609:618	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	65	theme	vapor	555:559	arg1	hydrophobicity					575:588	hydrophobicity	575:588	hydrophobicity	575:588	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	3	65	theme	vapor	555:559	arg1	properties					654:663	thermal, mechanical, and optical properties	621:663	thermal, mechanical, and optical properties	621:663	An experimental Central Composite Rotatable Design (CCRD) was used to evaluate the effect of these variables on different film's properties, i.e., water vapor permeability, hydrophobicity, moisture content, solubility, thermal, mechanical, and optical properties.
36144531	1	66	dep	Amaranthus	234:243	arg1	cruentus					245:252	cruentus	245:252	cruentus	245:252	This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents.
36144531	2	67	theme	various	347:353	arg1	concentrations					355:368	various concentrations	347:368	various concentrations	347:368	Alginate, glycerol, and amaranth grain phenolic compounds at various concentrations were used to produce the films.
36144531	4	68	theme	antioxidant	730:740	arg1	capacity					742:749	antioxidant capacity	730:749	antioxidant capacity	730:749	This study demonstrated that high phenolic compound content and antioxidant capacity were obtained from amaranth grain using ethanol as the extraction solvent.
36144531	6	69	theme	Principal	1222:1230	arg1	PCA					1252:1254	PCA	1252:1254	PCA	1252:1254	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	6	69	theme	Principal	1222:1230	arg1	Analysis					1242:1249	a Principal Component Analysis	1220:1249	a Principal Component Analysis (PCA)	1220:1255	For example, less flexible but more resistant and water-soluble films can be produced by increasing the alginate concentration, which was confirmed by a Principal Component Analysis (PCA) and Partial Least Squares (PLS) analysis.
36144531	5	70	theme	films	980:984	arg1	formulation					956:966	the formulation	952:966	the formulation of alginate films containing amaranth phenolic compounds	952:1023	Alginate films incorporating amaranth phenolic compounds were successfully manufactured, and this study can be used to tailor the formulation of alginate films containing amaranth phenolic compounds, depending on their final food application.
36144531	0	71	theme	Extracts	53:60	arg1	Valorization					0:11	Valorization	0:11	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.	0:112	Valorization of Amaranth (Amaranthus cruentus) Grain Extracts for the Development of Alginate-Based Active Films.
36144531	4	72	theme	extraction	806:815	arg1	solvent					817:823	the extraction solvent	802:823	the extraction solvent	802:823	This study demonstrated that high phenolic compound content and antioxidant capacity were obtained from amaranth grain using ethanol as the extraction solvent.
36144531	4	72	theme	extraction	806:815	arg1	ethanol					791:797	ethanol	791:797	ethanol	791:797	This study demonstrated that high phenolic compound content and antioxidant capacity were obtained from amaranth grain using ethanol as the extraction solvent.
36144531	1	73	theme	different	266:274	arg1	solvents					276:283	different solvents	266:283	different solvents	266:283	This research work investigates the development of alginate-based films incorporating phenolic compounds extracted from Amaranthus cruentus grain using different solvents.
36484847	0	0	theme	swine	112:116	arg1	cell					135:138	swine dental pulp stem cell	112:138	swine dental pulp stem cell for bone tissue engineering	112:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	4	1	from	presence	854:861	arg1	matrix					899:904	the 3d matrix	892:904	the 3d matrix of the scaffold	892:920	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	6	2	theme	proliferation	1064:1076	arg1	test					1078:1081	the cell viability and proliferation test	1041:1081	the cell viability and proliferation test	1041:1081	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	7	3	theme	bone	1475:1478	arg1	defects					1480:1486	bone defects	1475:1486	bone defects	1475:1486	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	2	4	theme	technique	632:640	arg1	use					606:608	use	606:608	use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h	606:742	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	7	5	from	tests	1446:1450	arg1	defects					1480:1486	bone defects	1475:1486	bone defects	1475:1486	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	2	6	theme	physical	574:581	arg1	bonds					583:587	physical bonds	574:587	physical bonds	574:587	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	1	7	dep	face	460:463	arg1	problem					470:476	this problem	465:476	this problem	465:476	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	8	8	theme	necessary	1607:1615	arg1	properties					1596:1605	osteoconductive properties	1580:1605	osteoconductive properties necessary for good regeneration of bone tissue	1580:1652	Likewise, this biomaterial was enhanced with the addition of HA, providing a scaffold with osteoconductive properties necessary for good regeneration of bone tissue.
36484847	0	9	theme	pulp	125:128	arg1	cell					135:138	swine dental pulp stem cell	112:138	swine dental pulp stem cell for bone tissue engineering	112:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	6	10	theme	cell	1257:1260	arg1	DPSCs					1263:1267	swine dental pulp stem cell (DPSCs)	1234:1268	swine dental pulp stem cell (DPSCs) followed by mineralization	1234:1295	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	1	11	theme	high	442:445	arg1	potential					447:455	a high potential	440:455	a high potential to face this problem	440:476	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	7	12	theme	complementary	1423:1435	arg1	tests					1446:1450	future complementary in vitro tests	1416:1450	future complementary in vitro tests	1416:1450	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	3	13	theme	scaffolds	797:805	arg1	properties					779:788	The physicochemical and bioactive properties	745:788	The physicochemical and bioactive properties of the scaffolds	745:805	The physicochemical and bioactive properties of the scaffolds were studied.
36484847	0	14	theme	dental	118:123	arg1	cell					135:138	swine dental pulp stem cell	112:138	swine dental pulp stem cell for bone tissue engineering	112:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	9	15	dep	abstract	1665:1672	arg1	Graphical					1655:1663	Graphical	1655:1663	Graphical	1655:1663	Graphical abstract.
36484847	2	16	theme	temperature	681:691	arg1	conditions					693:702	temperature conditions	681:702	temperature conditions of -58 °C and pressure of 1 Pa for 16 h	681:742	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	2	17	theme	lyophilization	617:630	arg1	technique					632:640	the lyophilization technique	613:640	the lyophilization technique	613:640	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	7	18	theme	future	1416:1421	arg1	tests					1446:1450	future complementary in vitro tests	1416:1450	future complementary in vitro tests	1416:1450	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	1	19	theme	bone	281:284	arg1	loss					286:289	bone loss	281:289	bone loss	281:289	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	6	20	theme	dental	1240:1245	arg1	DPSCs					1263:1267	swine dental pulp stem cell (DPSCs)	1234:1268	swine dental pulp stem cell (DPSCs) followed by mineralization	1234:1295	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	6	21	theme	pulp	1247:1250	arg1	DPSCs					1263:1267	swine dental pulp stem cell (DPSCs)	1234:1268	swine dental pulp stem cell (DPSCs) followed by mineralization	1234:1295	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	7	22	dep	in	1437:1438	arg1	vitro					1440:1444	vitro	1440:1444	vitro	1440:1444	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	6	23	theme	viability	1050:1058	arg1	test					1078:1081	the cell viability and proliferation test	1041:1081	the cell viability and proliferation test	1041:1081	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	0	24	theme	stem	130:133	arg1	cell					135:138	swine dental pulp stem cell	112:138	swine dental pulp stem cell for bone tissue engineering	112:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	1	25	theme	polymeric	354:362	arg1	biomaterial					364:374	a polymeric biomaterial	352:374	a polymeric biomaterial that mimics the osteogenic composition and structure	352:427	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	4	26	theme	FTIR	821:824	arg1	results					834:840	FTIR and TGA results	821:840	FTIR and TGA results	821:840	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	2	27	theme	porous	659:664	arg1	scaffolds					666:674	the 3D porous scaffolds	652:674	the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h	652:742	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	2	28	with	scaffolds	666:674	arg1	conditions					693:702	temperature conditions	681:702	temperature conditions of -58 °C and pressure of 1 Pa for 16 h	681:742	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	2	29	theme	1 Pa	730:733	arg1	-58 °C					707:712	-58 °C	707:712	-58 °C	707:712	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	2	29	theme	1 Pa	730:733	arg1	pressure					718:725	pressure	718:725	pressure	718:725	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	1	30	theme	clinical	200:207	arg1	need					209:212	a clinical need	198:212	a clinical need	198:212	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	6	31	theme	swine	1234:1238	arg1	DPSCs					1263:1267	swine dental pulp stem cell (DPSCs)	1234:1268	swine dental pulp stem cell (DPSCs) followed by mineralization	1234:1295	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	2	32	theme	3D	656:657	arg1	scaffolds					666:674	the 3D porous scaffolds	652:674	the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h	652:742	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	6	33	theme	alizarin	1128:1135	arg1	staining					1137:1144	alizarin staining	1128:1144	alizarin staining	1128:1144	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	7	34	theme	in	1456:1457	arg1	studies					1464:1470	in vivo studies	1456:1470	in vivo studies on bone defects	1456:1486	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	0	35	theme	3D	0:1	arg1	scaffolds					3:11	3D scaffolds	0:11	3D scaffolds of caprolactone/chitosan/polyvinyl	0:46	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	4	36	theme	TGA	830:832	arg1	results					834:840	FTIR and TGA results	821:840	FTIR and TGA results	821:840	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	7	37	theme	in	1437:1438	arg1	tests					1446:1450	future complementary in vitro tests	1416:1450	future complementary in vitro tests	1416:1450	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	0	38	theme	caprolactone/chitosan/polyvinyl	16:46	arg1	scaffolds					3:11	3D scaffolds	0:11	3D scaffolds of caprolactone/chitosan/polyvinyl	0:46	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	0	39	theme	bone	144:147	arg1	engineering					156:166	bone tissue engineering	144:166	bone tissue engineering	144:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	5	40	theme	good	1010:1013	arg1	viability					1020:1028	good cell viability	1010:1028	good cell viability	1010:1028	The scaffolds exhibited a morphology with pore size and interconnectivity that promote good cell viability.
36484847	1	41	theme	related	215:221	arg1	need					209:212	a clinical need	198:212	a clinical need	198:212	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	8	42	theme	bone	1642:1645	arg1	tissue					1647:1652	bone tissue	1642:1652	bone tissue	1642:1652	Likewise, this biomaterial was enhanced with the addition of HA, providing a scaffold with osteoconductive properties necessary for good regeneration of bone tissue.
36484847	6	43	theme	differentiation	1106:1120	arg1	test					1122:1125	the differentiation test	1102:1125	the differentiation test	1102:1125	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	4	44	from	matrix	899:904	arg1	presence					854:861	the presence	850:861	the presence of the initial components in the 3d matrix of the scaffold	850:920	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	8	45	theme	tissue	1647:1652	arg1	regeneration					1626:1637	good regeneration	1621:1637	good regeneration of bone tissue	1621:1652	Likewise, this biomaterial was enhanced with the addition of HA, providing a scaffold with osteoconductive properties necessary for good regeneration of bone tissue.
36484847	1	46	theme	bone	226:229	arg1	anomalies					258:266	congenital anomalies	247:266	congenital anomalies	247:266	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	1	46	theme	bone	226:229	arg1	defects					231:237	bone defects	226:237	bone defects such as congenital anomalies	226:266	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	5	47	with	morphology	949:958	arg1	size					970:973	pore size	965:973	pore size	965:973	The scaffolds exhibited a morphology with pore size and interconnectivity that promote good cell viability.
36484847	5	47	with	morphology	949:958	arg1	interconnectivity					979:995	interconnectivity	979:995	interconnectivity	979:995	The scaffolds exhibited a morphology with pore size and interconnectivity that promote good cell viability.
36484847	3	48	theme	physicochemical	749:763	arg1	properties					779:788	The physicochemical and bioactive properties	745:788	The physicochemical and bioactive properties of the scaffolds	745:805	The physicochemical and bioactive properties of the scaffolds were studied.
36484847	0	49	theme	tissue	149:154	arg1	engineering					156:166	bone tissue engineering	144:166	bone tissue engineering	144:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	7	50	contain	has	1365:1367	arg2	characteristics					1369:1383	characteristics	1369:1383	characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects	1369:1486	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	7	50	contain	has	1365:1367	arg1	scaffold					1327:1334	the Cs-PCL-PVA-HA scaffold	1309:1334	the Cs-PCL-PVA-HA scaffold stabilized by physical bonds	1309:1363	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	3	51	theme	bioactive	769:777	arg1	properties					779:788	The physicochemical and bioactive properties	745:788	The physicochemical and bioactive properties of the scaffolds	745:805	The physicochemical and bioactive properties of the scaffolds were studied.
36484847	0	52	theme	physical	85:92	arg1	bonds					94:98	physical bonds	85:98	physical bonds seeded with swine dental pulp stem cell for bone tissue engineering	85:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	4	53	theme	scaffold	913:920	arg1	matrix					899:904	the 3d matrix	892:904	the 3d matrix of the scaffold	892:920	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	4	54	theme	initial	870:876	arg1	components					878:887	the initial components	866:887	the initial components in the 3d matrix of the scaffold	866:920	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	1	55	theme	osteogenic	392:401	arg1	composition					403:413	the osteogenic composition	388:413	the osteogenic composition	388:413	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	7	56	theme	physical	1350:1357	arg1	bonds					1359:1363	physical bonds	1350:1363	physical bonds	1350:1363	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	1	57	dep	pathologies	304:314	arg1	is					345:346	is	345:346	is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem	345:476	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	7	58	theme	great	1398:1402	arg1	utility					1404:1410	great utility	1398:1410	great utility for future complementary in vitro tests and in vivo studies on bone defects	1398:1486	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	6	59	theme	stem	1252:1255	arg1	DPSCs					1263:1267	swine dental pulp stem cell (DPSCs)	1234:1268	swine dental pulp stem cell (DPSCs) followed by mineralization	1234:1295	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	2	60	theme	stabilized	542:551	arg1	hydrogel					553:560	a stabilized hydrogel	540:560	a stabilized hydrogel by means of physical bonds	540:587	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	5	61	theme	cell	1015:1018	arg1	viability					1020:1028	good cell viability	1010:1028	good cell viability	1010:1028	The scaffolds exhibited a morphology with pore size and interconnectivity that promote good cell viability.
36484847	0	62	dep	alcohol/hydroxyapatite	48:69	arg1	stabilized					71:80	stabilized	71:80	alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering	48:166	3D scaffolds of caprolactone/chitosan/polyvinyl alcohol/hydroxyapatite stabilized by physical bonds seeded with swine dental pulp stem cell for bone tissue engineering.
36484847	6	63	theme	scaffolds	1191:1199	arg1	ability					1158:1164	the ability	1154:1164	the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization	1154:1295	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	7	64	dep	in	1456:1457	arg1	vivo					1459:1462	vivo	1459:1462	vivo	1459:1462	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	4	65	from	components	878:887	arg1	matrix					899:904	the 3d matrix	892:904	the 3d matrix of the scaffold	892:920	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	6	66	theme	stabilized	1180:1189	arg1	scaffolds					1191:1199	physically stabilized scaffolds	1169:1199	physically stabilized scaffolds	1169:1199	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	1	67	with	trauma	269:274	arg1	loss					286:289	bone loss	281:289	bone loss	281:289	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	8	68	theme	good	1621:1624	arg1	regeneration					1626:1637	good regeneration	1621:1637	good regeneration of bone tissue	1621:1652	Likewise, this biomaterial was enhanced with the addition of HA, providing a scaffold with osteoconductive properties necessary for good regeneration of bone tissue.
36484847	7	69	from	studies	1464:1470	arg1	defects					1480:1486	bone defects	1475:1486	bone defects	1475:1486	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	4	70	theme	components	878:887	arg1	presence					854:861	the presence	850:861	the presence of the initial components in the 3d matrix of the scaffold	850:920	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	5	71	theme	pore	965:968	arg1	size					970:973	pore size	965:973	pore size	965:973	The scaffolds exhibited a morphology with pore size and interconnectivity that promote good cell viability.
36484847	2	72	theme	pressure	718:725	arg1	conditions					693:702	temperature conditions	681:702	temperature conditions of -58 °C and pressure of 1 Pa for 16 h	681:742	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	6	73	theme	Alamar	1084:1089	arg1	BlueTM					1091:1096	Alamar BlueTM	1084:1096	Alamar BlueTM	1084:1096	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	4	74	theme	3d	896:897	arg1	matrix					899:904	the 3d matrix	892:904	the 3d matrix of the scaffold	892:920	FTIR and TGA results confirm the presence of the initial components in the 3d matrix of the scaffold.
36484847	7	75	theme	Cs-PCL-PVA-HA	1313:1325	arg1	scaffold					1327:1334	the Cs-PCL-PVA-HA scaffold	1309:1334	the Cs-PCL-PVA-HA scaffold stabilized by physical bonds	1309:1363	Therefore, the Cs-PCL-PVA-HA scaffold stabilized by physical bonds has characteristics that suggest great utility for future complementary in vitro tests and in vivo studies on bone defects.
36484847	6	76	theme	cell	1045:1048	arg1	viability					1050:1058	the cell viability	1041:1058	the cell viability	1041:1058	Together, the cell viability and proliferation test, Alamar BlueTM and the differentiation test: alizarin staining, showed the ability of physically stabilized scaffolds to proliferate and differentiate swine dental pulp stem cell (DPSCs) followed by mineralization.
36484847	1	77	theme	congenital	247:256	arg1	anomalies					258:266	congenital anomalies	247:266	congenital anomalies	247:266	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	8	78	with	scaffold	1566:1573	arg1	properties					1596:1605	osteoconductive properties	1580:1605	osteoconductive properties necessary for good regeneration of bone tissue	1580:1652	Likewise, this biomaterial was enhanced with the addition of HA, providing a scaffold with osteoconductive properties necessary for good regeneration of bone tissue.
36484847	8	79	theme	osteoconductive	1580:1594	arg1	properties					1596:1605	osteoconductive properties	1580:1605	osteoconductive properties necessary for good regeneration of bone tissue	1580:1652	Likewise, this biomaterial was enhanced with the addition of HA, providing a scaffold with osteoconductive properties necessary for good regeneration of bone tissue.
36484847	2	80	theme	-58 °C	707:712	arg1	conditions					693:702	temperature conditions	681:702	temperature conditions of -58 °C and pressure of 1 Pa for 16 h	681:742	The method of obtaining these materials was first to prepare a stabilized hydrogel by means of physical bonds and then to make use of the lyophilization technique to obtain the 3D porous scaffolds with temperature conditions of -58 °C and pressure of 1 Pa for 16 h.
36484847	1	81	theme	Bone	169:172	arg1	Regeneration					174:185	Bone Regeneration	169:185	Bone Regeneration	169:185	Bone Regeneration represents a clinical need, related to bone defects such as congenital anomalies, trauma with bone loss, and/or some pathologies such as cysts or tumors This is why a polymeric biomaterial that mimics the osteogenic composition and structure represents a high potential to face this problem.
36484847	8	82	theme	HA	1550:1551	arg1	addition					1538:1545	the addition	1534:1545	the addition of HA	1534:1551	Likewise, this biomaterial was enhanced with the addition of HA, providing a scaffold with osteoconductive properties necessary for good regeneration of bone tissue.
37029634	6	0	theme	tartrate-resistant	1073:1090	arg1	TRAP					1110:1113	TRAP	1110:1113	TRAP	1110:1113	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	6	0	theme	tartrate-resistant	1073:1090	arg1	phosphatase					1097:1107	tartrate-resistant acid phosphatase	1073:1107	tartrate-resistant acid phosphatase (TRAP) staining	1073:1123	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	11	1	from	expressions	1840:1850	arg1	cells					1909:1913	the cells	1905:1913	the cells	1905:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	9	2	from	levels	1438:1443	arg1	macrophages					1601:1611	LPS-induced macrophages	1589:1611	LPS-induced macrophages	1589:1611	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	11	3	theme	anti-osteoclastogenesis	2135:2157	arg1	stronger					2179:2186	stronger	2179:2186	stronger	2179:2186	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	3	theme	anti-osteoclastogenesis	2135:2157	arg1	effect					2159:2164	the anti-osteoclastogenesis effect	2131:2164	the anti-osteoclastogenesis effect of ZBSO	2131:2172	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	10	4	theme	other	1780:1784	arg1	ZSOs					1786:1789	other ZSOs	1780:1789	other ZSOs	1780:1789	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	7	5	theme	oleic	1217:1221	arg1	constituent					1184:1194	the highest constituent	1172:1194	the highest constituent in ZSSO and ZDSO	1172:1211	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	7	5	theme	oleic	1217:1221	arg1	OA					1229:1230	OA	1229:1230	OA	1229:1230	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	7	5	theme	oleic	1217:1221	arg1	acid					1223:1226	oleic acid	1217:1226	oleic acid (OA)	1217:1231	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	1	6	theme	inhibitory	255:264	arg1	effects					266:272	inhibitory effects	255:272	inhibitory effects	255:272	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	4	7	theme	seed	622:625	arg1	ZBSO					632:635	ZBSO	632:635	ZBSO	632:635	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	4	7	theme	seed	622:625	arg1	oil					627:629	Zanthoxylum bungeanum maximum seed oil	592:629	Zanthoxylum bungeanum maximum seed oil (ZBSO)	592:636	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	3	8	theme	Zucc	526:529	arg1	kinds					476:480	three kinds	470:480	three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc	470:529	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
37029634	13	9	theme	anti-osteoclastogenesis	2455:2477	arg1	activities					2479:2488	anti-inflammatory and anti-osteoclastogenesis activities	2433:2488	anti-inflammatory and anti-osteoclastogenesis activities	2433:2488	All ZSOs tested had anti-inflammatory and anti-osteoclastogenesis activities.
37029634	1	10	theme	oil	197:199	arg1	kind					162:165	one kind	158:165	one kind	158:165	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	11	11	theme	TNF-α	1887:1891	arg1	number					1796:1801	The number	1792:1801	The number of RANKL-induced osteoclasts	1792:1830	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	11	theme	TNF-α	1887:1891	arg1	expressions					1840:1850	the expressions	1836:1850	the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells	1836:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	12	12	theme	major	2257:2261	arg1	kinds					2263:2267	three major kinds	2251:2267	three major kinds of ZSO	2251:2274	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	11	13	theme	kappa-B	1870:1876	arg1	number					1796:1801	The number	1792:1801	The number of RANKL-induced osteoclasts	1792:1830	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	13	theme	kappa-B	1870:1876	arg1	expressions					1840:1850	the expressions	1836:1850	the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells	1836:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	14	from	number	1796:1801	arg1	cells					1909:1913	the cells	1905:1913	the cells	1905:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	13	15	contain	had	2429:2431	arg1	ZSOs					2417:2420	All ZSOs	2413:2420	All ZSOs tested	2413:2427	All ZSOs tested had anti-inflammatory and anti-osteoclastogenesis activities.
37029634	13	15	contain	had	2429:2431	arg2	activities					2479:2488	anti-inflammatory and anti-osteoclastogenesis activities	2433:2488	anti-inflammatory and anti-osteoclastogenesis activities	2433:2488	All ZSOs tested had anti-inflammatory and anti-osteoclastogenesis activities.
37029634	3	16	theme	armatum	560:566	arg1	DC					568:569	Zanthoxylum armatum DC	548:569	Zanthoxylum armatum DC	548:569	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
37029634	3	16	theme	armatum	560:566	arg1	oil					536:538	oil	536:538	oil (ZSSO)	536:545	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
37029634	5	17	theme	ZSO	856:858	arg1	kinds					847:851	three kinds	841:851	three kinds of ZSO	841:858	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	11	18	theme	IL-6	1897:1900	arg1	number					1796:1801	The number	1792:1801	The number of RANKL-induced osteoclasts	1792:1830	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	18	theme	IL-6	1897:1900	arg1	expressions					1840:1850	the expressions	1836:1850	the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells	1836:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	19	from	IL-6	1897:1900	arg1	cells					1909:1913	the cells	1905:1913	the cells	1905:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	1	20	contain	had	251:253	arg2	effects					266:272	inhibitory effects	255:272	inhibitory effects	255:272	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	1	20	contain	had	251:253	arg1	kind					162:165	one kind	158:165	one kind	158:165	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	11	21	theme	RANKL-induced	2015:2027	arg1	osteoclasts					2029:2039	RANKL-induced osteoclasts	2015:2039	RANKL-induced osteoclasts after treated with ZBSO	2015:2063	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	12	22	theme	unsaturated	2309:2319	arg1	acids					2327:2331	unsaturated fatty acids	2309:2331	unsaturated fatty acids	2309:2331	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	0	23	theme	Zanthoxylum	79:89	arg1	Oil					106:108	Zanthoxylum bungeanum Seed Oil	79:108	Zanthoxylum bungeanum Seed Oil in vitro	79:117	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	11	24	theme	RANKL-induced	1806:1818	arg1	osteoclasts					1820:1830	RANKL-induced osteoclasts	1806:1830	RANKL-induced osteoclasts	1806:1830	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	8	25	theme	linoleic	1281:1288	arg1	LA					1296:1297	LA	1296:1297	LA	1296:1297	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	8	25	theme	linoleic	1281:1288	arg1	acid					1290:1293	linoleic acid	1281:1293	linoleic acid (LA)	1281:1298	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	0	26	theme	Seed	101:104	arg1	Oil					106:108	Zanthoxylum bungeanum Seed Oil	79:108	Zanthoxylum bungeanum Seed Oil in vitro	79:117	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	5	27	theme	kinds	847:851	arg1	effects					830:836	the inhibitory effects	815:836	the inhibitory effects of three kinds of ZSO on inflammation	815:874	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	10	28	theme	expression	1679:1688	arg1	levels					1690:1695	the expression levels	1675:1695	the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs	1675:1789	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	5	29	theme	expression	894:903	arg1	levels					905:910	the expression levels	890:910	the expression levels of inflammatory factors	890:934	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	11	30	from	TNF-α	1887:1891	arg1	cells					1909:1913	the cells	1905:1913	the cells	1905:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	31	theme	nuclear	1855:1861	arg1	kappa-B					1870:1876	nuclear factor kappa-B	1855:1876	nuclear factor kappa-B (NF-κB)	1855:1884	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	31	theme	nuclear	1855:1861	arg1	NF-κB					1879:1883	NF-κB	1879:1883	NF-κB	1879:1883	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	5	32	from	effects	830:836	arg1	inflammation					863:874	inflammation	863:874	inflammation	863:874	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	9	33	theme	inducible	1448:1456	arg1	iNOS					1471:1474	iNOS	1471:1474	iNOS	1471:1474	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	33	theme	inducible	1448:1456	arg1	beta					1573:1576	interleukin-1 beta	1559:1576	interleukin-1 beta (IL-1β)	1559:1584	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	33	theme	inducible	1448:1456	arg1	interleukin-6					1537:1549	interleukin-6	1537:1549	interleukin-6 (IL-6)	1537:1556	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	33	theme	inducible	1448:1456	arg1	factor-α					1519:1526	tumor necrosis factor-α	1504:1526	tumor necrosis factor-α (TNF-α)	1504:1534	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	33	theme	inducible	1448:1456	arg1	cyclooxygenase-2					1478:1493	cyclooxygenase-2	1478:1493	cyclooxygenase-2 (COX-2)	1478:1501	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	33	theme	inducible	1448:1456	arg1	synthase					1461:1468	inducible NO synthase	1448:1468	inducible NO synthase (iNOS)	1448:1475	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	7	34	theme	highest	1176:1182	arg1	constituent					1184:1194	the highest constituent	1172:1194	the highest constituent in ZSSO and ZDSO	1172:1211	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	7	34	theme	highest	1176:1182	arg1	acid					1223:1226	oleic acid	1217:1226	oleic acid (OA)	1217:1231	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	5	35	theme	factors	928:934	arg1	levels					905:910	the expression levels	890:910	the expression levels of inflammatory factors	890:934	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	14	36	theme	NF-κB	2576:2580	arg1	pathway					2582:2588	the NF-κB pathway	2572:2588	the NF-κB pathway	2572:2588	And their anti-osteoclastogenesis effects might be related to the suppression of the NF-κB pathway.
37029634	12	37	theme	fatty	2364:2368	arg1	acids					2370:2374	ω-3 polyunsaturated fatty acids	2344:2374	the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO	2294:2382	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	8	38	theme	α-Linolenic	1304:1314	arg1	ALA					1322:1324	ALA	1322:1324	ALA	1322:1324	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	8	38	theme	α-Linolenic	1304:1314	arg1	acid					1316:1319	α-Linolenic acid	1304:1319	α-Linolenic acid (ALA)	1304:1325	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	0	39	theme	Anti-Inflammatory	0:16	arg1	Activities					46:55	Anti-Inflammatory and Anti-Osteoclastogenesis Activities	0:55	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.	0:118	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	6	40	theme	anti-osteoclastogenesis	1022:1044	arg1	activities					1046:1055	the anti-osteoclastogenesis activities	1018:1055	the anti-osteoclastogenesis activities of them	1018:1063	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	12	41	theme	acid	2230:2233	arg1	compositions					2235:2246	the fatty acid compositions	2220:2246	the fatty acid compositions of three major kinds of ZSO	2220:2274	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	4	42	theme	acid	693:696	arg1	constituents					698:709	their fatty acid constituents	681:709	their fatty acid constituents	681:709	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	0	43	theme	Anti-Osteoclastogenesis	22:44	arg1	Activities					46:55	Anti-Inflammatory and Anti-Osteoclastogenesis Activities	0:55	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.	0:118	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	3	44	dep	Siebold	515:521	arg1	et					523:524	ZSO, Zanthoxylum schinifolium Siebold et	485:524	et	523:524	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
37029634	4	45	dep	Zanthoxylum	592:602	arg1	bungeanum					604:612	bungeanum	604:612	bungeanum	604:612	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	11	46	theme	μL/mL	1988:1992	arg1	concentration					1967:1979	the concentration	1963:1979	the concentration of 0.5 μL/mL	1963:1992	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	1	47	dep	Zanthoxylum	170:180	arg1	bungeanum					182:190	bungeanum	182:190	bungeanum	182:190	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	2	48	theme	kinds	358:362	arg1	activities					334:343	the anti-osteoclastogenesis activities	306:343	the anti-osteoclastogenesis activities of different kinds of ZSO	306:369	However, the anti-osteoclastogenesis activities of different kinds of ZSO are scarcely reported.
37029634	0	49	theme	Kinds	70:74	arg1	Activities					46:55	Anti-Inflammatory and Anti-Osteoclastogenesis Activities	0:55	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.	0:118	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	9	50	theme	tumor	1504:1508	arg1	synthase					1461:1468	inducible NO synthase	1448:1468	inducible NO synthase (iNOS)	1448:1475	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	50	theme	tumor	1504:1508	arg1	TNF-α					1529:1533	TNF-α	1529:1533	TNF-α	1529:1533	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	50	theme	tumor	1504:1508	arg1	factor-α					1519:1526	tumor necrosis factor-α	1504:1526	tumor necrosis factor-α (TNF-α)	1504:1534	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	12	51	theme	polyunsaturated	2348:2362	arg1	acids					2370:2374	ω-3 polyunsaturated fatty acids	2344:2374	the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO	2294:2382	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	4	52	theme	seed	572:575	arg1	oil					577:579	seed oil	572:579	seed oil (ZDSO)	572:586	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	4	52	theme	seed	572:575	arg1	ZDSO					582:585	ZDSO	582:585	ZDSO	582:585	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	8	53	from	acid	1290:1293	arg1	ZBSO					1330:1333	ZBSO	1330:1333	ZBSO	1330:1333	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	12	54	from	content	2298:2304	arg1	ZBSO					2379:2382	ZBSO	2379:2382	ZBSO	2379:2382	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	6	55	theme	acid	1092:1095	arg1	TRAP					1110:1113	TRAP	1110:1113	TRAP	1110:1113	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	6	55	theme	acid	1092:1095	arg1	phosphatase					1097:1107	tartrate-resistant acid phosphatase	1073:1107	tartrate-resistant acid phosphatase (TRAP) staining	1073:1123	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	13	56	theme	anti-inflammatory	2433:2449	arg1	activities					2479:2488	anti-inflammatory and anti-osteoclastogenesis activities	2433:2488	anti-inflammatory and anti-osteoclastogenesis activities	2433:2488	All ZSOs tested had anti-inflammatory and anti-osteoclastogenesis activities.
37029634	2	57	theme	ZSO	367:369	arg1	kinds					358:362	different kinds	348:362	different kinds of ZSO	348:369	However, the anti-osteoclastogenesis activities of different kinds of ZSO are scarcely reported.
37029634	8	58	from	acid	1316:1319	arg1	ZBSO					1330:1333	ZBSO	1330:1333	ZBSO	1330:1333	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	12	59	theme	fatty	2321:2325	arg1	acids					2327:2331	unsaturated fatty acids	2309:2331	unsaturated fatty acids	2309:2331	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	10	60	theme	inhibitory	1734:1743	arg1	stronger					1766:1773	stronger	1766:1773	stronger	1766:1773	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	10	60	theme	inhibitory	1734:1743	arg1	effects					1745:1751	the inhibitory effects	1730:1751	the inhibitory effects of ZSSO	1730:1759	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	5	61	used	used	798:801	arg2	macrophages					745:755	RAW264.7 macrophages	736:755	RAW264.7 macrophages induced by lipopolysaccharide (LPS)	736:791	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	2	62	theme	anti-osteoclastogenesis	310:332	arg1	activities					334:343	the anti-osteoclastogenesis activities	306:343	the anti-osteoclastogenesis activities of different kinds of ZSO	306:369	However, the anti-osteoclastogenesis activities of different kinds of ZSO are scarcely reported.
37029634	1	63	theme	Zanthoxylum	170:180	arg1	ZSO					202:204	ZSO	202:204	ZSO	202:204	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	1	63	theme	Zanthoxylum	170:180	arg1	oil					197:199	Zanthoxylum bungeanum seed oil	170:199	Zanthoxylum bungeanum seed oil (ZSO)	170:205	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	4	64	theme	maximum	614:620	arg1	ZBSO					632:635	ZBSO	632:635	ZBSO	632:635	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	4	64	theme	maximum	614:620	arg1	oil					627:629	Zanthoxylum bungeanum maximum seed oil	592:629	Zanthoxylum bungeanum maximum seed oil (ZBSO)	592:636	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	10	65	theme	μL/mL	1643:1647	arg1	concentration					1621:1633	the concentration	1617:1633	the concentration of 0.25 μL/mL	1617:1647	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	1	66	theme	seed	192:195	arg1	ZSO					202:204	ZSO	202:204	ZSO	202:204	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	1	66	theme	seed	192:195	arg1	oil					197:199	Zanthoxylum bungeanum seed oil	170:199	Zanthoxylum bungeanum seed oil (ZSO)	170:205	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	11	67	theme	other	2099:2103	arg1	ZSOs					2105:2108	other ZSOs	2099:2108	other ZSOs	2099:2108	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	68	theme	factor	1863:1868	arg1	kappa-B					1870:1876	nuclear factor kappa-B	1855:1876	nuclear factor kappa-B (NF-κB)	1855:1884	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	68	theme	factor	1863:1868	arg1	NF-κB					1879:1883	NF-κB	1879:1883	NF-κB	1879:1883	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	9	69	theme	μL/mL	1378:1382	arg1	concentration					1357:1369	the concentration	1353:1369	the concentration of 0.5 μL/mL	1353:1382	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	70	theme	interleukin-1	1559:1571	arg1	beta					1573:1576	interleukin-1 beta	1559:1576	interleukin-1 beta (IL-1β)	1559:1584	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	70	theme	interleukin-1	1559:1571	arg1	synthase					1461:1468	inducible NO synthase	1448:1468	inducible NO synthase (iNOS)	1448:1475	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	70	theme	interleukin-1	1559:1571	arg1	IL-1β					1579:1583	IL-1β	1579:1583	IL-1β	1579:1583	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	12	71	theme	ZSO	2272:2274	arg1	kinds					2263:2267	three major kinds	2251:2267	three major kinds of ZSO	2251:2274	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	12	72	theme	kinds	2263:2267	arg1	compositions					2235:2246	the fatty acid compositions	2220:2246	the fatty acid compositions of three major kinds of ZSO	2220:2274	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	5	73	theme	inhibitory	819:828	arg1	effects					830:836	the inhibitory effects	815:836	the inhibitory effects of three kinds of ZSO on inflammation	815:874	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	14	74	theme	pathway	2582:2588	arg1	the suppression					2553:2567	the suppression	2553:2567	the suppression of the NF-κB pathway	2553:2588	And their anti-osteoclastogenesis effects might be related to the suppression of the NF-κB pathway.
37029634	7	75	from	constituent	1184:1194	arg1	ZDSO					1208:1211	ZDSO	1208:1211	ZDSO	1208:1211	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	7	75	from	constituent	1184:1194	arg1	ZSSO					1199:1202	ZSSO	1199:1202	ZSSO	1199:1202	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	4	76	theme	Zanthoxylum	592:602	arg1	ZBSO					632:635	ZBSO	632:635	ZBSO	632:635	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	4	76	theme	Zanthoxylum	592:602	arg1	oil					627:629	Zanthoxylum bungeanum maximum seed oil	592:629	Zanthoxylum bungeanum maximum seed oil (ZBSO)	592:636	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	3	77	theme	Zanthoxylum	548:558	arg1	DC					568:569	Zanthoxylum armatum DC	548:569	Zanthoxylum armatum DC	548:569	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
37029634	3	77	theme	Zanthoxylum	548:558	arg1	oil					536:538	oil	536:538	oil (ZSSO)	536:545	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
37029634	1	78	theme	previous	124:131	arg1	study					133:137	Our previous study	120:137	Our previous study	120:137	Our previous study has exhibited that one kind of Zanthoxylum bungeanum seed oil (ZSO), extracted from Zanthoxylum bungeanum seed, had inhibitory effects on osteoclastogenesis.
37029634	11	79	theme	osteoclasts	1820:1830	arg1	number					1796:1801	The number	1792:1801	The number of RANKL-induced osteoclasts	1792:1830	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	79	theme	osteoclasts	1820:1830	arg1	expressions					1840:1850	the expressions	1836:1850	the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells	1836:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	9	80	theme	ZSO	1404:1406	arg1	kinds					1395:1399	all three kinds	1385:1399	all three kinds of ZSO	1385:1406	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	81	theme	LPS-induced	1589:1599	arg1	macrophages					1601:1611	LPS-induced macrophages	1589:1611	LPS-induced macrophages	1589:1611	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	4	82	theme	Soxhlet	658:664	arg1	extraction					666:675	Soxhlet extraction	658:675	Soxhlet extraction	658:675	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	0	83	theme	bungeanum	91:99	arg1	Oil					106:108	Zanthoxylum bungeanum Seed Oil	79:108	Zanthoxylum bungeanum Seed Oil in vitro	79:117	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	12	84	theme	acids	2327:2331	arg1	content					2298:2304	the content	2294:2304	the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO	2294:2382	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	12	84	theme	acids	2327:2331	arg1	highest					2393:2399	highest	2393:2399	highest	2393:2399	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	6	85	theme	RANKL-induced	958:970	arg1	osteoclastogenesis					972:989	RANKL-induced osteoclastogenesis	958:989	RANKL-induced osteoclastogenesis	958:989	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	11	86	theme	osteoclasts	2029:2039	arg1	number					2005:2010	the number	2001:2010	the number of RANKL-induced osteoclasts after treated with ZBSO	2001:2063	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	86	theme	osteoclasts	2029:2039	arg1	less					2070:2073	less	2070:2073	less	2070:2073	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	87	from	cells	1909:1913	arg1	number					1796:1801	The number	1792:1801	The number of RANKL-induced osteoclasts	1792:1830	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	87	from	cells	1909:1913	arg1	expressions					1840:1850	the expressions	1836:1850	the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells	1836:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	0	88	theme	Oil	106:108	arg1	Kinds					70:74	Different Kinds	60:74	Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro	60:117	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	9	89	theme	expression	1427:1436	arg1	levels					1438:1443	the expression levels	1423:1443	the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages	1423:1611	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	8	90	theme	While	1275:1279	arg1	LA					1296:1297	LA	1296:1297	LA	1296:1297	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	8	90	theme	While	1275:1279	arg1	acid					1290:1293	linoleic acid	1281:1293	linoleic acid (LA)	1281:1298	While linoleic acid (LA) and α-Linolenic acid (ALA) in ZBSO were dominant.
37029634	11	91	theme	ZBSO	2169:2172	arg1	stronger					2179:2186	stronger	2179:2186	stronger	2179:2186	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	11	91	theme	ZBSO	2169:2172	arg1	effect					2159:2164	the anti-osteoclastogenesis effect	2131:2164	the anti-osteoclastogenesis effect of ZBSO	2131:2172	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	12	92	dep	content	2298:2304	arg1	acids					2370:2374	ω-3 polyunsaturated fatty acids	2344:2374	the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO	2294:2382	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	5	93	theme	inflammatory	915:926	arg1	factors					928:934	inflammatory factors	915:934	inflammatory factors	915:934	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	6	94	theme	them	1060:1063	arg1	activities					1046:1055	the anti-osteoclastogenesis activities	1018:1055	the anti-osteoclastogenesis activities of them	1018:1063	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	11	95	theme	other	2193:2197	arg1	ZSOs					2199:2202	other ZSOs	2193:2202	other ZSOs	2193:2202	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	7	96	theme	GC-FID	1142:1147	arg1	results					1149:1155	The GC-FID results	1138:1155	The GC-FID results	1138:1155	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	9	97	theme	NO	1458:1459	arg1	iNOS					1471:1474	iNOS	1471:1474	iNOS	1471:1474	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	97	theme	NO	1458:1459	arg1	beta					1573:1576	interleukin-1 beta	1559:1576	interleukin-1 beta (IL-1β)	1559:1584	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	97	theme	NO	1458:1459	arg1	interleukin-6					1537:1549	interleukin-6	1537:1549	interleukin-6 (IL-6)	1537:1556	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	97	theme	NO	1458:1459	arg1	factor-α					1519:1526	tumor necrosis factor-α	1504:1526	tumor necrosis factor-α (TNF-α)	1504:1534	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	97	theme	NO	1458:1459	arg1	cyclooxygenase-2					1478:1493	cyclooxygenase-2	1478:1493	cyclooxygenase-2 (COX-2)	1478:1501	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	97	theme	NO	1458:1459	arg1	synthase					1461:1468	inducible NO synthase	1448:1468	inducible NO synthase (iNOS)	1448:1475	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	10	98	theme	COX-2	1709:1713	arg1	levels					1690:1695	the expression levels	1675:1695	the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs	1675:1789	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	4	99	theme	fatty	687:691	arg1	constituents					698:709	their fatty acid constituents	681:709	their fatty acid constituents	681:709	seed oil (ZDSO) and Zanthoxylum bungeanum maximum seed oil (ZBSO), were obtained with Soxhlet extraction and their fatty acid constituents were detected by GC-FID.
37029634	0	100	theme	Different	60:68	arg1	Kinds					70:74	Different Kinds	60:74	Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro	60:117	Anti-Inflammatory and Anti-Osteoclastogenesis Activities of Different Kinds of Zanthoxylum bungeanum Seed Oil in vitro.
37029634	3	101	theme	bone	427:430	arg1	loss					432:435	bone loss	427:435	bone loss	427:435	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
37029634	10	102	theme	iNOS	1700:1703	arg1	levels					1690:1695	the expression levels	1675:1695	the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs	1675:1789	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	5	103	theme	RAW264.7	736:743	arg1	macrophages					745:755	RAW264.7 macrophages	736:755	RAW264.7 macrophages induced by lipopolysaccharide (LPS)	736:791	RAW264.7 macrophages induced by lipopolysaccharide (LPS) were used to evaluate the inhibitory effects of three kinds of ZSO on inflammation via detecting the expression levels of inflammatory factors by RT-qPCR.
37029634	7	104	theme	palmitoleic	1237:1247	arg1	PLA					1255:1257	PLA	1255:1257	PLA	1255:1257	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	7	104	theme	palmitoleic	1237:1247	arg1	acid					1249:1252	palmitoleic acid	1237:1252	palmitoleic acid (PLA)	1237:1258	The GC-FID results exhibited that the highest constituent in ZSSO and ZDSO was oleic acid (OA) and palmitoleic acid (PLA), respectively.
37029634	12	105	theme	fatty	2224:2228	arg1	compositions					2235:2246	the fatty acid compositions	2220:2246	the fatty acid compositions of three major kinds of ZSO	2220:2274	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	2	106	theme	different	348:356	arg1	kinds					358:362	different kinds	348:362	different kinds of ZSO	348:369	However, the anti-osteoclastogenesis activities of different kinds of ZSO are scarcely reported.
37029634	14	107	theme	anti-osteoclastogenesis	2501:2523	arg1	effects					2525:2531	And their anti-osteoclastogenesis effects	2491:2531	effects	2525:2531	And their anti-osteoclastogenesis effects might be related to the suppression of the NF-κB pathway.
37029634	11	108	from	kappa-B	1870:1876	arg1	cells					1909:1913	the cells	1905:1913	the cells	1905:1913	The number of RANKL-induced osteoclasts and the expressions of nuclear factor kappa-B (NF-κB), TNF-α and IL-6 in the cells were decreased after being treated with ZSOs at the concentration of 0.5 μL/mL, while the number of RANKL-induced osteoclasts after treated with ZBSO were less than those treated with other ZSOs, this indicated that the anti-osteoclastogenesis effect of ZBSO were stronger than other ZSOs.
37029634	9	109	theme	synthase	1461:1468	arg1	levels					1438:1443	the expression levels	1423:1443	the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages	1423:1611	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	110	theme	necrosis	1510:1517	arg1	synthase					1461:1468	inducible NO synthase	1448:1468	inducible NO synthase (iNOS)	1448:1475	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	110	theme	necrosis	1510:1517	arg1	TNF-α					1529:1533	TNF-α	1529:1533	TNF-α	1529:1533	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	9	110	theme	necrosis	1510:1517	arg1	factor-α					1519:1526	tumor necrosis factor-α	1504:1526	tumor necrosis factor-α (TNF-α)	1504:1534	At the concentration of 0.5 μL/mL, all three kinds of ZSO could decrease the expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β) in LPS-induced macrophages.
37029634	6	111	theme	phosphatase	1097:1107	arg1	staining					1116:1123	tartrate-resistant acid phosphatase (TRAP) staining	1073:1123	tartrate-resistant acid phosphatase (TRAP) staining	1073:1123	Moreover, RANKL-induced osteoclastogenesis was applied to demonstrate the anti-osteoclastogenesis activities of them through tartrate-resistant acid phosphatase (TRAP) staining and RT-qPCR.
37029634	12	112	theme	ω-3	2344:2346	arg1	acids					2370:2374	ω-3 polyunsaturated fatty acids	2344:2374	the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO	2294:2382	In conclusion, the fatty acid compositions of three major kinds of ZSO were compared and the content of unsaturated fatty acids especially ω-3 polyunsaturated fatty acids in ZBSO were the highest among them.
37029634	10	113	theme	ZSSO	1756:1759	arg1	stronger					1766:1773	stronger	1766:1773	stronger	1766:1773	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	10	113	theme	ZSSO	1756:1759	arg1	effects					1745:1751	the inhibitory effects	1730:1751	the inhibitory effects of ZSSO	1730:1759	At the concentration of 0.25 μL/mL, only ZSSO could decrease the expression levels of iNOS and COX-2, which implied the inhibitory effects of ZSSO were stronger than other ZSOs.
37029634	3	114	theme	Siebold	515:521	arg1	Zucc					526:529	ZSO, Zanthoxylum schinifolium Siebold et Zucc	485:529	ZSO, Zanthoxylum schinifolium Siebold et Zucc	485:529	Since inflammation is related to bone loss and osteoporosis, in this study, three kinds of ZSO, Zanthoxylum schinifolium Siebold et Zucc seed oil (ZSSO), Zanthoxylum armatum DC.
36701279	6	0	theme	oxygen	1081:1086	arg1	species					1088:1094	reactive oxygen species	1072:1094	reactive oxygen species (ROS)	1072:1100	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	6	0	theme	oxygen	1081:1086	arg1	ROS					1097:1099	ROS	1097:1099	ROS	1097:1099	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	11	1	theme	ROS	1862:1864	arg1	mechanisms					1883:1892	the simulated ROS and inflammation mechanisms	1848:1892	the simulated ROS and inflammation mechanisms	1848:1892	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	1	2	theme	articular	248:256	arg1	cartilage					258:266	articular cartilage	248:266	articular cartilage	248:266	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	10	3	theme	numerical	1737:1745	arg1	predictions					1747:1757	The numerical predictions	1733:1757	The numerical predictions of cell death and PG loss	1733:1783	The numerical predictions of cell death and PG loss were supported by previous experimental findings.
36701279	6	4	theme	-driven	1144:1150	arg1	apoptosis					1152:1160	inflammatory cytokine (interleukin-1)-driven apoptosis	1107:1160	inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant	1107:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	6	5	from	necrosis	1018:1025	arg1	explant					1175:1181	cartilage explant	1165:1181	cartilage explant	1165:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	0	6	theme	Injury-related	0:13	arg1	death					20:24	Injury-related cell death	0:24	Injury-related cell death	0:24	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	5	7	theme	cell-mediated	819:831	arg1	mechanisms					845:854	the cell-mediated degradation mechanisms	815:854	the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes	815:940	Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes.
36701279	9	8	theme	culture	1674:1680	arg1	medium					1682:1687	the culture medium	1670:1687	the culture medium	1670:1687	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	6	9	from	apoptosis	1028:1036	arg1	explant					1175:1181	cartilage explant	1165:1181	cartilage explant	1165:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	10	10	theme	death	1767:1771	arg1	predictions					1747:1757	The numerical predictions	1733:1757	The numerical predictions of cell death and PG loss	1733:1783	The numerical predictions of cell death and PG loss were supported by previous experimental findings.
36701279	6	11	dep	-driven	1144:1150	arg1	cytokine					1120:1127	inflammatory cytokine	1107:1127	inflammatory cytokine	1107:1127	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	9	12	theme	closer	1560:1565	arg1	activity					1531:1538	proteolytic activity	1519:1538	proteolytic activity	1519:1538	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	0	13	theme	oxygen	117:122	arg1	species					124:130	reactive oxygen species	108:130	reactive oxygen species	108:130	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	1	14	theme	common	186:191	arg1	Osteoarthritis					161:174	Osteoarthritis	161:174	Osteoarthritis (OA)	161:179	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	1	14	theme	common	186:191	arg1	disease					209:215	a common musculoskeletal disease	184:215	a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life	184:309	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	11	15	theme	inflammation	1870:1881	arg1	mechanisms					1883:1892	the simulated ROS and inflammation mechanisms	1848:1892	the simulated ROS and inflammation mechanisms	1848:1892	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	8	16	theme	PG	1260:1261	arg1	degeneration					1263:1274	Biomechanically triggered PG degeneration	1234:1274	Biomechanically triggered PG degeneration	1234:1274	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	11	17	theme	PG	1942:1943	arg1	content					1945:1951	the PG content	1938:1951	the PG content than localized necrosis	1938:1975	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	8	18	theme	diffusion-driven	1419:1434	arg1	degeneration					1439:1450	interleukin-1 diffusion-driven PG degeneration	1405:1450	interleukin-1 diffusion-driven PG degeneration	1405:1450	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	0	19	theme	inflammatory	137:148	arg1	cytokines					150:158	inflammatory cytokines	137:158	inflammatory cytokines	137:158	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	10	20	theme	PG	1777:1778	arg1	loss					1780:1783	PG loss	1777:1783	PG loss	1777:1783	The numerical predictions of cell death and PG loss were supported by previous experimental findings.
36701279	9	21	theme	PG	1721:1722	arg1	content					1724:1730	PG content	1721:1730	PG content	1721:1730	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	12	22	theme	cartilage	2071:2079	arg1	degeneration					2081:2092	early cartilage degeneration mechanisms and the efficacy of interventions	2065:2137	degeneration	2081:2092	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	10	23	theme	experimental	1812:1823	arg1	findings					1825:1832	previous experimental findings	1803:1832	previous experimental findings	1803:1832	The numerical predictions of cell death and PG loss were supported by previous experimental findings.
36701279	3	24	from	dysfunction	495:505	arg1	cartilage					526:534	cartilage	526:534	cartilage	526:534	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	6	25	theme	finite	986:991	arg1	model					1001:1005	a 2-D mechanobiological finite element model	962:1005	a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant	962:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	0	26	from	death	20:24	arg1	cartilage					61:69	articular cartilage	51:69	articular cartilage	51:69	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	8	27	theme	excessive	1308:1316	arg1	production					1322:1331	excessive ROS production	1308:1331	excessive ROS production	1308:1331	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	0	28	theme	Numerical	72:80	arg1	model					82:86	Numerical model	72:86	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.	0:159	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	6	29	theme	2-D	964:966	arg1	model					1001:1005	a 2-D mechanobiological finite element model	962:1005	a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant	962:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	12	30	theme	early	2065:2069	arg1	degeneration					2081:2092	early cartilage degeneration mechanisms and the efficacy of interventions	2065:2137	degeneration	2081:2092	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	4	31	theme	tissue	699:704	arg1	synthesis					706:714	tissue synthesis	699:714	tissue synthesis	699:714	These processes, influenced by biomechanical and inflammatory stimuli, disturb the normal cell-regulated balance between tissue synthesis and degeneration.
36701279	11	32	theme	longer-lasting	1898:1911	arg1	effects					1913:1919	longer-lasting effects	1898:1919	longer-lasting effects (over 3 days)	1898:1933	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	9	33	theme	PG	1544:1545	arg1	biosynthesis					1547:1558	PG biosynthesis	1544:1558	PG biosynthesis	1544:1558	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	6	34	from	apoptosis	1152:1160	arg1	explant					1175:1181	cartilage explant	1165:1181	cartilage explant	1165:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	5	35	theme	computational	743:755	arg1	models					775:780	Previous computational mechanobiological models	734:780	Previous computational mechanobiological models	734:780	Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes.
36701279	1	36	theme	joint	269:273	arg1	pain					275:278	joint pain	269:278	joint pain	269:278	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	5	37	theme	tissue-level	907:918	arg1	changes					934:940	tissue-level compositional changes	907:940	tissue-level compositional changes	907:940	Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes.
36701279	4	38	theme	normal	661:666	arg1	balance					683:689	the normal cell-regulated balance	657:689	the normal cell-regulated balance between tissue synthesis and degeneration	657:731	These processes, influenced by biomechanical and inflammatory stimuli, disturb the normal cell-regulated balance between tissue synthesis and degeneration.
36701279	3	39	theme	PTOA	413:416	arg1	etiology					401:408	The etiology	397:408	The etiology of PTOA	397:416	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	3	40	from	death	517:521	arg1	cartilage					526:534	cartilage	526:534	cartilage	526:534	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	2	41	theme	post-traumatic	371:384	arg1	PTOA					390:393	PTOA	390:393	PTOA	390:393	When OA develops after a joint injury, it is designated as post-traumatic OA (PTOA).
36701279	2	41	theme	post-traumatic	371:384	arg1	OA					386:387	post-traumatic OA	371:387	post-traumatic OA (PTOA)	371:394	When OA develops after a joint injury, it is designated as post-traumatic OA (PTOA).
36701279	2	41	theme	post-traumatic	371:384	arg1	it					351:352	it	351:352	it	351:352	When OA develops after a joint injury, it is designated as post-traumatic OA (PTOA).
36701279	0	42	theme	proteoglycan	30:41	arg1	loss					43:46	proteoglycan loss	30:46	proteoglycan loss	30:46	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	1	43	theme	decreased	285:293	arg1	quality					295:301	decreased quality	285:301	decreased quality of life	285:309	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	3	44	theme	first	550:554	arg1	signs					556:560	the first signs	546:560	the first signs of the disease	546:575	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	9	45	theme	tissue	1592:1597	arg1	levels					1574:1579	the levels	1570:1579	the levels of healthy tissue	1570:1597	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	12	46	theme	PTOA	2151:2154	arg1	progression					2156:2166	PTOA progression	2151:2166	PTOA progression	2151:2166	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	9	47	theme	pro-inflammatory	1604:1619	arg1	cytokines					1621:1629	pro-inflammatory cytokines	1604:1629	pro-inflammatory cytokines	1604:1629	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	3	48	theme	disease	569:575	arg1	signs					556:560	the first signs	546:560	the first signs of the disease	546:575	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	6	49	theme	species	1088:1094	arg1	production					1058:1067	excessive production	1048:1067	excessive production of reactive oxygen species (ROS)	1048:1100	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	11	50	theme	simulated	1852:1860	arg1	ROS					1862:1864	simulated ROS	1852:1864	simulated ROS	1852:1864	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	6	51	theme	reactive	1072:1079	arg1	species					1088:1094	reactive oxygen species	1072:1094	reactive oxygen species (ROS)	1072:1100	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	6	51	theme	reactive	1072:1079	arg1	ROS					1097:1099	ROS	1097:1099	ROS	1097:1099	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	1	52	theme	cartilage	258:266	arg1	deterioration					231:243	deterioration	231:243	deterioration of articular cartilage, joint pain, and decreased quality of life	231:309	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	12	53	theme	mechanobiological	1982:1998	arg1	tool					2046:2049	a numerical tool	2034:2049	a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression	2034:2166	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	12	53	theme	mechanobiological	1982:1998	arg1	model					2000:2004	The mechanobiological model	1978:2004	The mechanobiological model presented here	1978:2019	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	7	54	theme	PG	1198:1199	arg1	simulated					1223:1231	simulated	1223:1231	simulated	1223:1231	The resulting PG loss over 30 days was simulated.
36701279	7	54	theme	PG	1198:1199	arg1	loss					1201:1204	The resulting PG loss	1184:1204	The resulting PG loss over 30 days	1184:1217	The resulting PG loss over 30 days was simulated.
36701279	3	55	theme	cell	490:493	arg1	dysfunction					495:505	cell dysfunction	490:505	cell dysfunction	490:505	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	5	56	theme	degradation	833:843	arg1	mechanisms					845:854	the cell-mediated degradation mechanisms	815:854	the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes	815:940	Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes.
36701279	12	57	theme	interventions	2125:2137	arg1	degeneration					2081:2092	early cartilage degeneration mechanisms and the efficacy of interventions	2065:2137	degeneration	2081:2092	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	12	57	theme	interventions	2125:2137	arg1	efficacy					2113:2120	the efficacy	2109:2120	the efficacy of interventions	2109:2137	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	12	58	dep	degeneration	2081:2092	arg1	mechanisms					2094:2103	mechanisms	2094:2103	mechanisms	2094:2103	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	10	59	theme	cell	1762:1765	arg1	death					1767:1771	cell death	1762:1771	cell death	1762:1771	The numerical predictions of cell death and PG loss were supported by previous experimental findings.
36701279	8	60	theme	interleukin-1	1405:1417	arg1	degeneration					1439:1450	interleukin-1 diffusion-driven PG degeneration	1405:1450	interleukin-1 diffusion-driven PG degeneration	1405:1450	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	6	61	theme	inflammatory	1107:1118	arg1	cytokine					1120:1127	inflammatory cytokine	1107:1127	inflammatory cytokine	1107:1127	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	3	62	theme	cell	512:515	arg1	death					517:521	cell death	512:521	cell death	512:521	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	1	63	theme	musculoskeletal	193:207	arg1	Osteoarthritis					161:174	Osteoarthritis	161:174	Osteoarthritis (OA)	161:179	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	1	63	theme	musculoskeletal	193:207	arg1	disease					209:215	a common musculoskeletal disease	184:215	a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life	184:309	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	9	64	theme	partial	1701:1707	arg1	recovery					1709:1716	partial recovery	1701:1716	partial recovery of PG content	1701:1730	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	8	65	theme	PG	1436:1437	arg1	degeneration					1439:1450	interleukin-1 diffusion-driven PG degeneration	1405:1450	interleukin-1 diffusion-driven PG degeneration	1405:1450	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	8	66	theme	triggered	1250:1258	arg1	degeneration					1263:1274	Biomechanically triggered PG degeneration	1234:1274	Biomechanically triggered PG degeneration	1234:1274	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	10	67	theme	loss	1780:1783	arg1	predictions					1747:1757	The numerical predictions	1733:1757	The numerical predictions of cell death and PG loss	1733:1783	The numerical predictions of cell death and PG loss were supported by previous experimental findings.
36701279	4	68	theme	biomechanical	609:621	arg1	stimuli					640:646	biomechanical and inflammatory stimuli	609:646	biomechanical and inflammatory stimuli	609:646	These processes, influenced by biomechanical and inflammatory stimuli, disturb the normal cell-regulated balance between tissue synthesis and degeneration.
36701279	3	69	from	loss	484:487	arg1	cartilage					526:534	cartilage	526:534	cartilage	526:534	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	11	70	theme	localized	1958:1966	arg1	necrosis					1968:1975	localized necrosis	1958:1975	localized necrosis	1958:1975	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	9	71	theme	content	1724:1730	arg1	recovery					1709:1716	partial recovery	1701:1716	partial recovery of PG content	1701:1730	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	4	72	theme	inflammatory	627:638	arg1	stimuli					640:646	biomechanical and inflammatory stimuli	609:646	biomechanical and inflammatory stimuli	609:646	These processes, influenced by biomechanical and inflammatory stimuli, disturb the normal cell-regulated balance between tissue synthesis and degeneration.
36701279	10	73	theme	previous	1803:1810	arg1	findings					1825:1832	previous experimental findings	1803:1832	previous experimental findings	1803:1832	The numerical predictions of cell death and PG loss were supported by previous experimental findings.
36701279	6	74	theme	element	993:999	arg1	model					1001:1005	a 2-D mechanobiological finite element model	962:1005	a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant	962:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	6	75	theme	cartilage	1165:1173	arg1	explant					1175:1181	cartilage explant	1165:1181	cartilage explant	1165:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	8	76	theme	cell	1293:1296	arg1	necrosis					1298:1305	cell necrosis	1293:1305	cell necrosis	1293:1305	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	3	77	from	proteoglycan	466:477	arg1	cartilage					526:534	cartilage	526:534	cartilage	526:534	The etiology of PTOA remains poorly understood, but it is known that proteoglycan (PG) loss, cell dysfunction, and cell death in cartilage are among the first signs of the disease.
36701279	7	78	theme	resulting	1188:1196	arg1	simulated					1223:1231	simulated	1223:1231	simulated	1223:1231	The resulting PG loss over 30 days was simulated.
36701279	7	78	theme	resulting	1188:1196	arg1	loss					1201:1204	The resulting PG loss	1184:1204	The resulting PG loss over 30 days	1184:1217	The resulting PG loss over 30 days was simulated.
36701279	6	79	theme	mechanobiological	968:984	arg1	model					1001:1005	a 2-D mechanobiological finite element model	962:1005	a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant	962:1181	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	12	80	theme	numerical	2036:2044	arg1	tool					2046:2049	a numerical tool	2034:2049	a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression	2034:2166	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	12	80	theme	numerical	2036:2044	arg1	model					2000:2004	The mechanobiological model	1978:2004	The mechanobiological model presented here	1978:2019	The mechanobiological model presented here may serve as a numerical tool for assessing early cartilage degeneration mechanisms and the efficacy of interventions to mitigate PTOA progression.
36701279	0	81	dep	death	20:24	arg1	model					82:86	Numerical model	72:86	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.	0:159	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	9	82	theme	proteolytic	1519:1529	arg1	activity					1531:1538	proteolytic activity	1519:1538	proteolytic activity	1519:1538	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	4	83	theme	cell-regulated	668:681	arg1	balance					683:689	the normal cell-regulated balance	657:689	the normal cell-regulated balance between tissue synthesis and degeneration	657:731	These processes, influenced by biomechanical and inflammatory stimuli, disturb the normal cell-regulated balance between tissue synthesis and degeneration.
36701279	8	84	theme	ROS	1318:1320	arg1	production					1322:1331	excessive ROS production	1308:1331	excessive ROS production	1308:1331	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	0	85	theme	reactive	108:115	arg1	species					124:130	reactive oxygen species	108:130	reactive oxygen species	108:130	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	11	86	contain	had	1894:1896	arg1	mechanisms					1883:1892	the simulated ROS and inflammation mechanisms	1848:1892	the simulated ROS and inflammation mechanisms	1848:1892	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	11	86	contain	had	1894:1896	arg2	effects					1913:1919	longer-lasting effects	1898:1919	longer-lasting effects (over 3 days)	1898:1933	Furthermore, the simulated ROS and inflammation mechanisms had longer-lasting effects (over 3 days) on the PG content than localized necrosis.
36701279	0	87	from	loss	43:46	arg1	cartilage					61:69	articular cartilage	51:69	articular cartilage	51:69	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	5	88	theme	Previous	734:741	arg1	models					775:780	Previous computational mechanobiological models	734:780	Previous computational mechanobiological models	734:780	Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes.
36701279	6	89	theme	excessive	1048:1056	arg1	production					1058:1067	excessive production	1048:1067	excessive production of reactive oxygen species (ROS)	1048:1100	Here, we developed a 2-D mechanobiological finite element model to predict necrosis, apoptosis following excessive production of reactive oxygen species (ROS), and inflammatory cytokine (interleukin-1)-driven apoptosis in cartilage explant.
36701279	8	90	theme	cell	1338:1341	arg1	apoptosis					1343:1351	cell apoptosis	1338:1351	cell apoptosis	1338:1351	Biomechanically triggered PG degeneration, associated with cell necrosis, excessive ROS production, and cell apoptosis, was predicted to be localized near a lesion, while interleukin-1 diffusion-driven PG degeneration was manifested more globally.
36701279	5	91	theme	mechanobiological	757:773	arg1	models					775:780	Previous computational mechanobiological models	734:780	Previous computational mechanobiological models	734:780	Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes.
36701279	1	92	theme	pain	275:278	arg1	deterioration					231:243	deterioration	231:243	deterioration of articular cartilage, joint pain, and decreased quality of life	231:309	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	5	93	theme	compositional	920:932	arg1	changes					934:940	tissue-level compositional changes	907:940	tissue-level compositional changes	907:940	Previous computational mechanobiological models have not explicitly incorporated the cell-mediated degradation mechanisms triggered by an injury that eventually can lead to tissue-level compositional changes.
36701279	0	94	theme	articular	51:59	arg1	cartilage					61:69	articular cartilage	51:69	articular cartilage	51:69	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
36701279	1	95	theme	quality	295:301	arg1	deterioration					231:243	deterioration	231:243	deterioration of articular cartilage, joint pain, and decreased quality of life	231:309	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	9	96	theme	healthy	1584:1590	arg1	tissue					1592:1597	healthy tissue	1584:1597	healthy tissue	1584:1597	Interestingly, the model also showed proteolytic activity and PG biosynthesis closer to the levels of healthy tissue when pro-inflammatory cytokines were rapidly inhibited or cleared from the culture medium, leading to partial recovery of PG content.
36701279	1	97	theme	life	306:309	arg1	quality					295:301	decreased quality	285:301	decreased quality of life	285:309	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	1	97	theme	life	306:309	arg1	cartilage					258:266	articular cartilage	248:266	articular cartilage	248:266	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	1	97	theme	life	306:309	arg1	pain					275:278	joint pain	269:278	joint pain	269:278	Osteoarthritis (OA) is a common musculoskeletal disease that leads to deterioration of articular cartilage, joint pain, and decreased quality of life.
36701279	2	98	theme	joint	337:341	arg1	injury					343:348	a joint injury	335:348	a joint injury	335:348	When OA develops after a joint injury, it is designated as post-traumatic OA (PTOA).
36701279	0	99	theme	cell	15:18	arg1	death					20:24	Injury-related cell death	0:24	Injury-related cell death	0:24	Injury-related cell death and proteoglycan loss in articular cartilage: Numerical model combining necrosis, reactive oxygen species, and inflammatory cytokines.
35033121	8	0	theme	short-chain	1068:1078	arg1	acids					1086:1090	short-chain fatty acids	1068:1090	Fecal short-chain fatty acids (SCFAs) levels	1062:1105	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	8	0	theme	short-chain	1068:1078	arg1	SCFAs					1093:1097	SCFAs	1093:1097	SCFAs	1093:1097	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	16	1	theme	gut	2570:2572	arg1	function					2582:2589	the gut barrier function	2566:2589	the gut barrier function	2566:2589	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	10	2	theme	DSS-induced	1446:1456	arg1	colitis					1458:1464	DSS-induced colitis	1446:1464	DSS-induced colitis in mice	1446:1472	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	7	3	theme	colonic	1045:1051	arg1	tissues					1053:1059	colonic tissues	1045:1059	colonic tissues	1045:1059	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	8	4	theme	acids	1086:1090	arg1	levels					1100:1105	Fecal short-chain fatty acids (SCFAs) levels	1062:1105	Fecal short-chain fatty acids (SCFAs) levels	1062:1105	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	16	5	dep	P.	2407:2408	arg1	pentosaceus					2410:2420	pentosaceus	2410:2420	pentosaceus	2410:2420	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	17	6	theme	pentosaceus	2606:2616	arg1	8330					2623:2626	P. pentosaceus CECT 8330	2603:2626	P. pentosaceus CECT 8330	2603:2626	Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.
35033121	17	6	theme	pentosaceus	2606:2616	arg1	probiotic					2653:2661	probiotic	2653:2661	probiotic	2653:2661	Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.
35033121	8	7	theme	16S	1177:1179	arg1	sequencing					1191:1200	16S rRNA gene sequencing	1177:1200	16S rRNA gene sequencing	1177:1200	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	7	8	theme	junction	1018:1025	arg1	proteins					1027:1034	the tight junction proteins	1008:1034	the tight junction proteins (TJP) in colonic tissues	1008:1059	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	7	8	theme	junction	1018:1025	arg1	TJP					1037:1039	TJP	1037:1039	TJP	1037:1039	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	8	9	theme	microbiota	1111:1120	arg1	composition					1122:1132	microbiota composition	1111:1132	microbiota composition	1111:1132	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	8	10	theme	gene	1186:1189	arg1	sequencing					1191:1200	16S rRNA gene sequencing	1177:1200	16S rRNA gene sequencing	1177:1200	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	13	11	theme	several	1958:1964	arg1	norank_f_Muribaculaceae					2004:2026	norank_f_Muribaculaceae	2004:2026	norank_f_Muribaculaceae	2004:2026	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	11	theme	several	1958:1964	arg1	Bifidobacterium					2044:2058	Bifidobacterium	2044:2058	Bifidobacterium	2044:2058	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	11	theme	several	1958:1964	arg1	bacteria					1977:1984	several protective bacteria genera	1958:1991	several protective bacteria genera	1958:1991	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	11	theme	several	1958:1964	arg1	Dubosiella					2065:2074	Dubosiella	2065:2074	Dubosiella	2065:2074	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	11	theme	several	1958:1964	arg1	Lactobacillus					2029:2041	Lactobacillus	2029:2041	Lactobacillus	2029:2041	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	9	12	theme	microbial	1362:1370	arg1	profiles					1383:1390	the microbial functional profiles	1358:1390	the microbial functional profiles	1358:1390	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	11	13	theme	treated	1745:1751	arg1	mice					1753:1756	DSS treated mice	1741:1756	DSS treated mice	1741:1756	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	13	14	theme	bacteria	1977:1984	arg1	abundances					1944:1953	relative abundances	1935:1953	relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella	1935:2074	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	14	theme	bacteria	1977:1984	arg1	level					1925:1929	the fecal SCFAs level	1909:1929	the fecal SCFAs level	1909:1929	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	5	15	theme	fecal	797:801	arg1	conditions					803:812	fecal conditions	797:812	fecal conditions	797:812	Weight, fecal conditions, colon length and histopathological changes were examined.
35033121	12	16	theme	Treg	1846:1849	arg1	cells					1851:1855	Treg cells	1846:1855	Treg cells	1846:1855	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	10	17	theme	disease	1516:1522	arg1	loss					1510:1513	the weight loss	1499:1513	the weight loss	1499:1513	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	17	theme	disease	1516:1522	arg1	DAI					1540:1542	DAI	1540:1542	DAI	1540:1542	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	17	theme	disease	1516:1522	arg1	index					1533:1537	disease activity index	1516:1537	disease activity index	1516:1537	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	6	18	theme	flow	883:886	arg1	cytometry					888:896	flow cytometry	883:896	flow cytometry	883:896	ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio.
35033121	0	19	from	responses	114:122	arg1	mice					127:130	mice	127:130	mice	127:130	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	11	20	theme	serum	1641:1645	arg1	levels					1647:1652	the serum levels	1637:1652	the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6)	1637:1706	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	7	21	theme	Western	975:981	arg1	blot					983:986	Western blot	975:986	Western blot	975:986	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	11	22	dep	cytokines	1673:1681	arg1	IL-1β					1691:1695	IL-1β	1691:1695	IL-1β	1691:1695	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	11	22	dep	cytokines	1673:1681	arg1	IL-6					1702:1705	IL-6	1702:1705	IL-6	1702:1705	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	11	22	dep	cytokines	1673:1681	arg1	TNF-α					1684:1688	TNF-α	1684:1688	TNF-α	1684:1688	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	11	22	dep	cytokines	1673:1681	arg1	cytokines					1673:1681	proinflammatory cytokines	1657:1681	proinflammatory cytokines (TNF-α, IL-1β, and IL-6)	1657:1706	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	1	23	theme	inflammatory	253:264	arg1	IBD					282:284	IBD	282:284	IBD	282:284	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	1	23	theme	inflammatory	253:264	arg1	diseases					272:279	inflammatory bowel diseases	253:279	inflammatory bowel diseases (IBD)	253:285	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	15	24	theme	pentosaceus	2310:2320	arg1	8330					2327:2330	P. pentosaceus CECT 8330	2307:2330	P. pentosaceus CECT 8330	2307:2330	The KEGG and COG pathway analysis revealed that P. pentosaceus CECT 8330 could partially recover the metabolic pathways altered by DSS.
35033121	1	25	theme	Compelling	144:153	arg1	evidences					155:163	BACKGROUND Compelling evidences	133:163	BACKGROUND Compelling evidences	133:163	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	16	26	theme	P.	2407:2408	arg1	CONCLUSIONS					2395:2405	CONCLUSIONS	2395:2405	CONCLUSIONS P. pentosaceus	2395:2420	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	5	27	theme	histopathological	832:848	arg1	changes					850:856	histopathological changes	832:856	histopathological changes	832:856	Weight, fecal conditions, colon length and histopathological changes were examined.
35033121	4	28	theme	pentosaceus	666:676	arg1	8330					683:686	P. pentosaceus CECT 8330	663:686	P. pentosaceus CECT 8330 (5 × 108 CFU/day)	663:704	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	4	28	theme	pentosaceus	666:676	arg1	5 × 108 CFU/day					689:703	5 × 108 CFU/day	689:703	5 × 108 CFU/day	689:703	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	11	29	theme	cytokines	1673:1681	arg1	levels					1647:1652	the serum levels	1637:1652	the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6)	1637:1706	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	0	30	theme	intestinal	81:90	arg1	microbiota					92:101	the intestinal microbiota	77:101	the intestinal microbiota	77:101	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	13	31	theme	SCFAs	1919:1923	arg1	level					1925:1929	the fecal SCFAs level	1909:1929	the fecal SCFAs level	1909:1929	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	12	32	theme	CECT	1774:1777	arg1	8330					1779:1782	P. pentosaceus CECT 8330	1759:1782	P. pentosaceus CECT 8330	1759:1782	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	1	33	theme	gut	183:185	arg1	dysbiosis					198:206	gut microbiota dysbiosis	183:206	gut microbiota dysbiosis	183:206	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	6	34	theme	T	953:953	arg1	Treg					962:965	Treg	962:965	Treg	962:965	ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio.
35033121	6	34	theme	T	953:953	arg1	cells					955:959	regulatory T cells	942:959	regulatory T cells (Treg)	942:966	ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio.
35033121	13	35	theme	pentosaceus	1877:1887	arg1	8330					1894:1897	P. pentosaceus CECT 8330	1874:1897	P. pentosaceus CECT 8330	1874:1897	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	10	36	dep	P.	1401:1402	arg1	pentosaceus					1404:1414	pentosaceus	1404:1414	pentosaceus	1404:1414	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	9	37	theme	Genes	1243:1247	arg1	Cluster					1272:1278	Cluster	1272:1278	Cluster of orthologous groups of proteins (COG)	1272:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	3	38	dep	pentosaceus	492:502	arg1	pentosaceus					508:518	P. pentosaceus	505:518	P. pentosaceus	505:518	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	16	39	theme	barrier	2574:2580	arg1	function					2582:2589	the gut barrier function	2566:2589	the gut barrier function	2566:2589	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	0	40	theme	Pediococcus	0:10	arg1	8330					29:32	Pediococcus pentosaceus CECT 8330	0:32	Pediococcus pentosaceus CECT 8330	0:32	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	9	41	theme	Genomes	1253:1259	arg1	Cluster					1272:1278	Cluster	1272:1278	Cluster of orthologous groups of proteins (COG)	1272:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	12	42	theme	P.	1759:1760	arg1	8330					1779:1782	P. pentosaceus CECT 8330	1759:1782	P. pentosaceus CECT 8330	1759:1782	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	14	43	theme	genera	2127:2132	arg1	abundances					2104:2113	the increased abundances	2090:2113	the increased abundances of bacteria genera	2090:2132	Furthermore, the increased abundances of bacteria genera were positively correlated with IL-10 and SCFAs levels, and negatively associated with IL-6, IL-1β, and TNF-α, respectively.
35033121	0	44	theme	CECT	24:27	arg1	8330					29:32	Pediococcus pentosaceus CECT 8330	0:32	Pediococcus pentosaceus CECT 8330	0:32	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	10	45	from	colitis	1458:1464	arg1	mice					1469:1472	mice	1469:1472	mice	1469:1472	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	9	46	used	used	1342:1345	arg2	Cluster					1272:1278	Cluster	1272:1278	Cluster of orthologous groups of proteins (COG)	1272:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	9	46	used	used	1342:1345	arg2	Encyclopedia					1227:1238	Encyclopedia	1227:1238	Encyclopedia	1227:1238	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	3	47	from	colitis	572:578	arg1	mice					583:586	mice	583:586	mice	583:586	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	17	48	theme	intestinal	2677:2686	arg1	inflammation					2688:2699	intestinal inflammation	2677:2699	intestinal inflammation	2677:2699	Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.
35033121	3	49	theme	Pediococcus	480:490	arg1	strain					472:477	a probiotic strain	460:477	a probiotic strain	460:477	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	3	49	theme	Pediococcus	480:490	arg1	pentosaceus					492:502	Pediococcus pentosaceus	480:502	Pediococcus pentosaceus (P. pentosaceus) CECT 8330	480:529	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	10	50	theme	8330	1421:1424	arg1	treatment					1426:1434	8330 treatment	1421:1434	8330 treatment	1421:1434	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	13	51	theme	P.	1874:1875	arg1	8330					1894:1897	P. pentosaceus CECT 8330	1874:1897	P. pentosaceus CECT 8330	1874:1897	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	10	52	theme	length	1583:1588	arg1	shortening					1590:1599	colon length shortening	1577:1599	colon length shortening	1577:1599	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	52	theme	length	1583:1588	arg1	loss					1510:1513	the weight loss	1499:1513	the weight loss	1499:1513	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	9	53	theme	groups	1295:1300	arg1	Cluster					1272:1278	Cluster	1272:1278	Cluster of orthologous groups of proteins (COG)	1272:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	16	54	theme	immunological	2538:2550	arg1	profiles					2552:2559	immunological profiles	2538:2559	immunological profiles	2538:2559	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	11	55	theme	pentosaceus	1605:1615	arg1	8330					1622:1625	P. pentosaceus CECT 8330	1602:1625	P. pentosaceus CECT 8330	1602:1625	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	4	56	theme	phosphate-buffered	628:645	arg1	saline					647:652	phosphate-buffered saline	628:652	phosphate-buffered saline (PBS)	628:658	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	4	56	theme	phosphate-buffered	628:645	arg1	PBS					655:657	PBS	655:657	PBS	655:657	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	9	57	theme	proteins	1305:1312	arg1	proteins					1305:1312	proteins	1305:1312	proteins (COG)	1305:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	9	57	theme	proteins	1305:1312	arg1	groups					1295:1300	orthologous groups	1283:1300	orthologous groups of proteins (COG)	1283:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	9	57	theme	proteins	1305:1312	arg1	COG					1315:1317	COG	1315:1317	COG	1315:1317	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	12	58	theme	colon	1860:1864	arg1	tissue					1866:1871	colon tissue	1860:1871	colon tissue	1860:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	11	59	theme	IL-10	1732:1736	arg1	level					1723:1727	level	1723:1727	level of IL-10	1723:1736	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	1	60	theme	diseases	272:279	arg1	pathogenesis					237:248	the pathogenesis	233:248	the pathogenesis of inflammatory bowel diseases (IBD)	233:285	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	8	61	theme	Fecal	1062:1066	arg1	levels					1100:1105	Fecal short-chain fatty acids (SCFAs) levels	1062:1105	Fecal short-chain fatty acids (SCFAs) levels	1062:1105	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	12	62	from	tissue	1866:1871	arg1	expression					1800:1809	the expression	1796:1809	the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue	1796:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	16	63	theme	gut	2498:2500	arg1	composition					2512:2522	the gut microbial composition	2494:2522	the gut microbial composition	2494:2522	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	9	64	theme	pathway	1320:1326	arg1	analysis					1328:1335	pathway analysis	1320:1335	pathway analysis	1320:1335	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	8	65	theme	fatty	1080:1084	arg1	acids					1086:1090	short-chain fatty acids	1068:1090	Fecal short-chain fatty acids (SCFAs) levels	1062:1105	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	8	65	theme	fatty	1080:1084	arg1	SCFAs					1093:1097	SCFAs	1093:1097	SCFAs	1093:1097	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	3	66	theme	CECT	521:524	arg1	strain					472:477	a probiotic strain	460:477	a probiotic strain	460:477	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	3	66	theme	CECT	521:524	arg1	pentosaceus					492:502	Pediococcus pentosaceus	480:502	Pediococcus pentosaceus (P. pentosaceus) CECT 8330	480:529	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	1	67	theme	microbiota	187:196	arg1	dysbiosis					198:206	gut microbiota dysbiosis	183:206	gut microbiota dysbiosis	183:206	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	12	68	theme	cells	1851:1855	arg1	ZO-1					1814:1817	ZO-1	1814:1817	ZO-1	1814:1817	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	12	68	theme	cells	1851:1855	arg1	Occludin					1820:1827	Occludin	1820:1827	Occludin	1820:1827	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	12	68	theme	cells	1851:1855	arg1	ratio					1837:1841	the ratio	1833:1841	the ratio of Treg cells in colon tissue	1833:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	17	69	theme	P.	2603:2604	arg1	8330					2623:2626	P. pentosaceus CECT 8330	2603:2626	P. pentosaceus CECT 8330	2603:2626	Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.
35033121	17	69	theme	P.	2603:2604	arg1	probiotic					2653:2661	probiotic	2653:2661	probiotic	2653:2661	Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.
35033121	12	70	theme	Occludin	1820:1827	arg1	expression					1800:1809	the expression	1796:1809	the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue	1796:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	8	71	theme	targeted	1151:1158	arg1	metabolomics					1160:1171	targeted metabolomics	1151:1171	targeted metabolomics	1151:1171	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	7	72	theme	tight	1012:1016	arg1	proteins					1027:1034	the tight junction proteins	1008:1034	the tight junction proteins (TJP) in colonic tissues	1008:1059	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	7	72	theme	tight	1012:1016	arg1	TJP					1037:1039	TJP	1037:1039	TJP	1037:1039	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	5	73	theme	colon	815:819	arg1	length					821:826	colon length	815:826	colon length	815:826	Weight, fecal conditions, colon length and histopathological changes were examined.
35033121	7	74	used	used	992:995	arg2	blot					983:986	Western blot	975:986	Western blot	975:986	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	6	75	theme	cytokines	928:936	arg1	ratio					968:972	the cytokines and regulatory T cells (Treg) ratio	924:972	the cytokines and regulatory T cells (Treg) ratio	924:972	ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio.
35033121	17	76	theme	CECT	2618:2621	arg1	8330					2623:2626	P. pentosaceus CECT 8330	2603:2626	P. pentosaceus CECT 8330	2603:2626	Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.
35033121	17	76	theme	CECT	2618:2621	arg1	probiotic					2653:2661	probiotic	2653:2661	probiotic	2653:2661	Therefore, P. pentosaceus CECT 8330 may serve as a promising probiotic to ameliorate intestinal inflammation.
35033121	10	77	theme	weight	1503:1508	arg1	loss					1510:1513	the weight loss	1499:1513	the weight loss	1499:1513	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	77	theme	weight	1503:1508	arg1	shortening					1590:1599	colon length shortening	1577:1599	colon length shortening	1577:1599	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	77	theme	weight	1503:1508	arg1	score					1545:1549	score	1545:1549	score	1545:1549	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	77	theme	weight	1503:1508	arg1	index					1533:1537	disease activity index	1516:1537	disease activity index	1516:1537	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	77	theme	weight	1503:1508	arg1	damage					1565:1570	histological damage	1552:1570	histological damage	1552:1570	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	1	78	theme	critical	216:223	arg1	role					225:228	a critical role	214:228	a critical role	214:228	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	0	79	from	microbiota	92:101	arg1	mice					127:130	mice	127:130	mice	127:130	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	4	80	theme	C57BL/6	597:603	arg1	mice					605:608	METHODS C57BL/6 mice	589:608	METHODS C57BL/6 mice	589:608	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	13	81	theme	protective	1966:1975	arg1	norank_f_Muribaculaceae					2004:2026	norank_f_Muribaculaceae	2004:2026	norank_f_Muribaculaceae	2004:2026	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	81	theme	protective	1966:1975	arg1	Bifidobacterium					2044:2058	Bifidobacterium	2044:2058	Bifidobacterium	2044:2058	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	81	theme	protective	1966:1975	arg1	bacteria					1977:1984	several protective bacteria genera	1958:1991	several protective bacteria genera	1958:1991	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	81	theme	protective	1966:1975	arg1	Dubosiella					2065:2074	Dubosiella	2065:2074	Dubosiella	2065:2074	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	13	81	theme	protective	1966:1975	arg1	Lactobacillus					2029:2041	Lactobacillus	2029:2041	Lactobacillus	2029:2041	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	3	82	theme	-induced	563:570	arg1	colitis					572:578	dextran sulfate sodium (DSS)-induced colitis	535:578	dextran sulfate sodium (DSS)-induced colitis in mice	535:586	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	9	83	theme	functional	1372:1381	arg1	profiles					1383:1390	the microbial functional profiles	1358:1390	the microbial functional profiles	1358:1390	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	16	84	theme	microbial	2502:2510	arg1	composition					2512:2522	the gut microbial composition	2494:2522	the gut microbial composition	2494:2522	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	12	85	theme	ratio	1837:1841	arg1	expression					1800:1809	the expression	1796:1809	the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue	1796:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	10	86	theme	activity	1524:1531	arg1	loss					1510:1513	the weight loss	1499:1513	the weight loss	1499:1513	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	86	theme	activity	1524:1531	arg1	DAI					1540:1542	DAI	1540:1542	DAI	1540:1542	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	86	theme	activity	1524:1531	arg1	index					1533:1537	disease activity index	1516:1537	disease activity index	1516:1537	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	3	87	dep	CECT	521:524	arg1	8330					526:529	8330	526:529	8330	526:529	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	12	88	theme	ZO-1	1814:1817	arg1	expression					1800:1809	the expression	1796:1809	the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue	1796:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	4	89	theme	CECT	678:681	arg1	8330					683:686	P. pentosaceus CECT 8330	663:686	P. pentosaceus CECT 8330 (5 × 108 CFU/day)	663:704	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	4	89	theme	CECT	678:681	arg1	5 × 108 CFU/day					689:703	5 × 108 CFU/day	689:703	5 × 108 CFU/day	689:703	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	13	90	theme	relative	1935:1942	arg1	abundances					1944:1953	relative abundances	1935:1953	relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella	1935:2074	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	1	91	theme	BACKGROUND	133:142	arg1	evidences					155:163	BACKGROUND Compelling evidences	133:163	BACKGROUND Compelling evidences	133:163	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	3	92	theme	strain	472:477	arg1	effect					450:455	the protective effect	435:455	the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice	435:586	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	15	93	theme	CECT	2322:2325	arg1	8330					2327:2330	P. pentosaceus CECT 8330	2307:2330	P. pentosaceus CECT 8330	2307:2330	The KEGG and COG pathway analysis revealed that P. pentosaceus CECT 8330 could partially recover the metabolic pathways altered by DSS.
35033121	16	94	theme	8330	2427:2430	arg1	administration					2432:2445	8330 administration	2427:2445	8330 administration	2427:2445	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	16	95	theme	DSS-induced	2460:2470	arg1	colitis					2472:2478	the DSS-induced colitis	2456:2478	the DSS-induced colitis	2456:2478	CONCLUSIONS P. pentosaceus CECT 8330 administration protects the DSS-induced colitis and modulates the gut microbial composition and function, immunological profiles, and the gut barrier function.
35033121	13	96	theme	fecal	1913:1917	arg1	SCFAs					1919:1923	the fecal SCFAs	1909:1923	the fecal SCFAs level	1909:1929	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	15	97	theme	P.	2307:2308	arg1	8330					2327:2330	P. pentosaceus CECT 8330	2307:2330	P. pentosaceus CECT 8330	2307:2330	The KEGG and COG pathway analysis revealed that P. pentosaceus CECT 8330 could partially recover the metabolic pathways altered by DSS.
35033121	6	98	theme	cells	955:959	arg1	ratio					968:972	the cytokines and regulatory T cells (Treg) ratio	924:972	the cytokines and regulatory T cells (Treg) ratio	924:972	ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio.
35033121	4	99	theme	P.	663:664	arg1	8330					683:686	P. pentosaceus CECT 8330	663:686	P. pentosaceus CECT 8330 (5 × 108 CFU/day)	663:704	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	4	99	theme	P.	663:664	arg1	5 × 108 CFU/day					689:703	5 × 108 CFU/day	689:703	5 × 108 CFU/day	689:703	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	14	100	theme	SCFAs	2176:2180	arg1	levels					2182:2187	IL-10 and SCFAs levels	2166:2187	IL-10 and SCFAs levels	2166:2187	Furthermore, the increased abundances of bacteria genera were positively correlated with IL-10 and SCFAs levels, and negatively associated with IL-6, IL-1β, and TNF-α, respectively.
35033121	0	101	theme	immune	107:112	arg1	responses					114:122	immune responses	107:122	immune responses in mice	107:130	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	15	102	theme	pathway	2276:2282	arg1	analysis					2284:2291	The KEGG and COG pathway analysis	2259:2291	analysis	2284:2291	The KEGG and COG pathway analysis revealed that P. pentosaceus CECT 8330 could partially recover the metabolic pathways altered by DSS.
35033121	6	103	theme	regulatory	942:951	arg1	Treg					962:965	Treg	962:965	Treg	962:965	ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio.
35033121	6	103	theme	regulatory	942:951	arg1	cells					955:959	regulatory T cells	942:959	regulatory T cells (Treg)	942:966	ELISA and flow cytometry were applied to determine the cytokines and regulatory T cells (Treg) ratio.
35033121	2	104	theme	alternative	339:349	arg1	probiotics					393:402	probiotics	393:402	probiotics	393:402	Therapies for targeting the microbiota may provide alternative options for the treatment of IBD, such as probiotics.
35033121	2	104	theme	alternative	339:349	arg1	options					351:357	alternative options	339:357	alternative options	339:357	Therapies for targeting the microbiota may provide alternative options for the treatment of IBD, such as probiotics.
35033121	14	105	theme	IL-10	2166:2170	arg1	levels					2182:2187	IL-10 and SCFAs levels	2166:2187	IL-10 and SCFAs levels	2166:2187	Furthermore, the increased abundances of bacteria genera were positively correlated with IL-10 and SCFAs levels, and negatively associated with IL-6, IL-1β, and TNF-α, respectively.
35033121	11	106	theme	proinflammatory	1657:1671	arg1	IL-1β					1691:1695	IL-1β	1691:1695	IL-1β	1691:1695	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	11	106	theme	proinflammatory	1657:1671	arg1	IL-6					1702:1705	IL-6	1702:1705	IL-6	1702:1705	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	11	106	theme	proinflammatory	1657:1671	arg1	TNF-α					1684:1688	TNF-α	1684:1688	TNF-α	1684:1688	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	11	106	theme	proinflammatory	1657:1671	arg1	cytokines					1673:1681	proinflammatory cytokines	1657:1681	proinflammatory cytokines (TNF-α, IL-1β, and IL-6)	1657:1706	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	13	107	theme	CECT	1889:1892	arg1	8330					1894:1897	P. pentosaceus CECT 8330	1874:1897	P. pentosaceus CECT 8330	1874:1897	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	8	108	theme	rRNA	1181:1184	arg1	sequencing					1191:1200	16S rRNA gene sequencing	1177:1200	16S rRNA gene sequencing	1177:1200	Fecal short-chain fatty acids (SCFAs) levels and microbiota composition were analyzed by targeted metabolomics and 16S rRNA gene sequencing, respectively.
35033121	3	109	theme	protective	439:448	arg1	effect					450:455	the protective effect	435:455	the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice	435:586	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	0	110	theme	pentosaceus	12:22	arg1	8330					29:32	Pediococcus pentosaceus CECT 8330	0:32	Pediococcus pentosaceus CECT 8330	0:32	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	12	111	from	ratio	1837:1841	arg1	tissue					1866:1871	colon tissue	1860:1871	colon tissue	1860:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	12	112	theme	pentosaceus	1762:1772	arg1	8330					1779:1782	P. pentosaceus CECT 8330	1759:1782	P. pentosaceus CECT 8330	1759:1782	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	15	113	theme	metabolic	2360:2368	arg1	pathways					2370:2377	the metabolic pathways	2356:2377	the metabolic pathways altered by DSS	2356:2392	The KEGG and COG pathway analysis revealed that P. pentosaceus CECT 8330 could partially recover the metabolic pathways altered by DSS.
35033121	7	114	from	proteins	1027:1034	arg1	tissues					1053:1059	colonic tissues	1045:1059	colonic tissues	1045:1059	Western blot was used to examine the tight junction proteins (TJP) in colonic tissues.
35033121	10	115	theme	P.	1401:1402	arg1	RESULTS					1393:1399	RESULTS	1393:1399	RESULTS P. pentosaceus	1393:1414	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	14	116	theme	bacteria	2118:2125	arg1	genera					2127:2132	bacteria genera	2118:2132	bacteria genera	2118:2132	Furthermore, the increased abundances of bacteria genera were positively correlated with IL-10 and SCFAs levels, and negatively associated with IL-6, IL-1β, and TNF-α, respectively.
35033121	3	117	theme	probiotic	462:470	arg1	strain					472:477	a probiotic strain	460:477	a probiotic strain	460:477	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	3	117	theme	probiotic	462:470	arg1	pentosaceus					492:502	Pediococcus pentosaceus	480:502	Pediococcus pentosaceus (P. pentosaceus) CECT 8330	480:529	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	0	118	theme	DSS-induced	43:53	arg1	colitis					55:61	DSS-induced colitis	43:61	DSS-induced colitis	43:61	Pediococcus pentosaceus CECT 8330 protects DSS-induced colitis and regulates the intestinal microbiota and immune responses in mice.
35033121	4	119	theme	colitis	763:769	arg1	induction					771:779	colitis induction	763:779	colitis induction by DSS	763:786	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	3	120	from	effect	450:455	arg1	colitis					572:578	dextran sulfate sodium (DSS)-induced colitis	535:578	dextran sulfate sodium (DSS)-induced colitis in mice	535:586	Here, we aimed to investigate the protective effect of a probiotic strain, Pediococcus pentosaceus (P. pentosaceus) CECT 8330, on dextran sulfate sodium (DSS)-induced colitis in mice.
35033121	10	121	theme	histological	1552:1563	arg1	loss					1510:1513	the weight loss	1499:1513	the weight loss	1499:1513	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	121	theme	histological	1552:1563	arg1	damage					1565:1570	histological damage	1552:1570	histological damage	1552:1570	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	12	122	from	Occludin	1820:1827	arg1	tissue					1866:1871	colon tissue	1860:1871	colon tissue	1860:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	15	123	theme	COG	2272:2274	arg1	analysis					2284:2291	The KEGG and COG pathway analysis	2259:2291	analysis	2284:2291	The KEGG and COG pathway analysis revealed that P. pentosaceus CECT 8330 could partially recover the metabolic pathways altered by DSS.
35033121	9	124	dep	Encyclopedia	1227:1238	arg1	analysis					1328:1335	pathway analysis	1320:1335	pathway analysis	1320:1335	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	11	125	theme	CECT	1617:1620	arg1	8330					1622:1625	P. pentosaceus CECT 8330	1602:1625	P. pentosaceus CECT 8330	1602:1625	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	9	126	theme	orthologous	1283:1293	arg1	proteins					1305:1312	proteins	1305:1312	proteins (COG)	1305:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	9	126	theme	orthologous	1283:1293	arg1	groups					1295:1300	orthologous groups	1283:1300	orthologous groups of proteins (COG)	1283:1318	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	9	126	theme	orthologous	1283:1293	arg1	COG					1315:1317	COG	1315:1317	COG	1315:1317	The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Cluster of orthologous groups of proteins (COG) pathway analysis were used to predict the microbial functional profiles.
35033121	12	127	from	ZO-1	1814:1817	arg1	tissue					1866:1871	colon tissue	1860:1871	colon tissue	1860:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	13	128	dep	bacteria	1977:1984	arg1	genera					1986:1991	genera	1986:1991	several protective bacteria genera	1958:1991	P. pentosaceus CECT 8330 increased the fecal SCFAs level and relative abundances of several protective bacteria genera, including norank_f_Muribaculaceae, Lactobacillus, Bifidobacterium, and Dubosiella.
35033121	11	129	theme	P.	1602:1603	arg1	8330					1622:1625	P. pentosaceus CECT 8330	1602:1625	P. pentosaceus CECT 8330	1602:1625	P. pentosaceus CECT 8330 decreased the serum levels of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), and increased level of IL-10 in DSS treated mice.
35033121	10	130	theme	colon	1577:1581	arg1	shortening					1590:1599	colon length shortening	1577:1599	colon length shortening	1577:1599	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	10	130	theme	colon	1577:1581	arg1	loss					1510:1513	the weight loss	1499:1513	the weight loss	1499:1513	RESULTS P. pentosaceus CECT 8330 treatment protected DSS-induced colitis in mice as evidenced by reducing the weight loss, disease activity index (DAI) score, histological damage, and colon length shortening.
35033121	12	131	from	expression	1800:1809	arg1	tissue					1866:1871	colon tissue	1860:1871	colon tissue	1860:1871	P. pentosaceus CECT 8330 upregulated the expression of ZO-1, Occludin and the ratio of Treg cells in colon tissue.
35033121	4	132	theme	METHODS	589:595	arg1	mice					605:608	METHODS C57BL/6 mice	589:608	METHODS C57BL/6 mice	589:608	METHODS C57BL/6 mice were administered phosphate-buffered saline (PBS) or P. pentosaceus CECT 8330 (5 × 108 CFU/day) once daily by gavage for 5 days prior to or 2 days after colitis induction by DSS.
35033121	14	133	theme	increased	2094:2102	arg1	abundances					2104:2113	the increased abundances	2090:2113	the increased abundances of bacteria genera	2090:2132	Furthermore, the increased abundances of bacteria genera were positively correlated with IL-10 and SCFAs levels, and negatively associated with IL-6, IL-1β, and TNF-α, respectively.
35033121	2	134	theme	IBD	380:382	arg1	treatment					367:375	the treatment	363:375	the treatment of IBD	363:382	Therapies for targeting the microbiota may provide alternative options for the treatment of IBD, such as probiotics.
35033121	1	135	theme	bowel	266:270	arg1	IBD					282:284	IBD	282:284	IBD	282:284	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
35033121	1	135	theme	bowel	266:270	arg1	diseases					272:279	inflammatory bowel diseases	253:279	inflammatory bowel diseases (IBD)	253:285	BACKGROUND Compelling evidences demonstrated that gut microbiota dysbiosis plays a critical role in the pathogenesis of inflammatory bowel diseases (IBD).
37223362	8	0	theme	Short-chain	1620:1630	arg1	acid					1638:1641	Short-chain fatty acid	1620:1641	Short-chain fatty acid analysis of faecal water	1620:1666	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	4	1	from	abundance	654:662	arg1	environment					737:747	a complex mammalian gut environment	713:747	a complex mammalian gut environment	713:747	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	5	2	theme	amplicon	1268:1275	arg1	sequencing					1277:1286	16s rRNA amplicon sequencing	1259:1286	16s rRNA amplicon sequencing	1259:1286	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	6	3	theme	formerly	1409:1416	arg1	Phocaeicola					1396:1406	the genus Phocaeicola	1386:1406	the genus Phocaeicola (formerly Bacteroides)	1386:1429	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	6	3	theme	formerly	1409:1416	arg1	Bacteroides					1418:1428	formerly Bacteroides	1409:1428	formerly Bacteroides	1409:1428	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	1	4	theme	Milk	169:172	arg1	Oligosaccharides					174:189	Human Milk Oligosaccharides	163:189	Human Milk Oligosaccharides (HMOs)	163:196	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	1	4	theme	Milk	169:172	arg1	HMOs					192:195	HMOs	192:195	HMOs	192:195	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	1	4	theme	Milk	169:172	arg1	glycans					202:208	glycans	202:208	glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health	202:350	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	6	5	theme	Phocaeicola	1396:1406	arg1	abundance					1373:1381	the relative abundance	1360:1381	the relative abundance of the genus Phocaeicola (formerly Bacteroides)	1360:1429	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	8	6	theme	isobutyrate	1713:1723	arg1	levels					1725:1730	acetate, butyrate and isobutyrate levels	1691:1730	acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus	1691:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	4	7	theme	specific	629:636	arg1	HMOs					638:641	specific HMOs	629:641	specific HMOs	629:641	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	3	8	theme	gut	583:585	arg1	microbiota					587:596	the gut microbiota	579:596	the gut microbiota	579:596	However, some species of Bacteroidaceae also degrade HMOs, which may prompt selection also of these species in the gut microbiota.
37223362	6	9	theme	Lacrimispora	1471:1482	arg1	decrease					1449:1456	a concomitant decrease	1435:1456	a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster)	1435:1518	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	6	9	theme	Lacrimispora	1471:1482	arg1	increase					1348:1355	an increase	1345:1355	an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides)	1345:1429	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	5	10	from	abundance	1146:1154	arg1	samples					1192:1198	faecal samples	1185:1198	faecal samples	1185:1198	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	8	11	from	decrease	1679:1686	arg1	levels					1725:1730	acetate, butyrate and isobutyrate levels	1691:1730	acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus	1691:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	5	12	theme	absolute	1124:1131	arg1	abundance					1146:1154	both the absolute and relative abundance	1115:1154	abundance	1146:1154	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	0	13	theme	levels	155:160	arg1	reduction					133:141	a reduction	131:141	a reduction of butyrate levels	131:160	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	8	14	theme	faecal	1655:1660	arg1	water					1662:1666	faecal water	1655:1666	faecal water	1655:1666	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	4	15	theme	complex	715:721	arg1	environment					737:747	a complex mammalian gut environment	713:747	a complex mammalian gut environment	713:747	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	9	16	theme	HMO-driven	1855:1864	arg1	selection					1881:1889	HMO-driven Bacteroidaceae selection	1855:1889	HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia	1855:1974	This study highlights HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia.
37223362	5	17	theme	relative	1137:1144	arg1	abundance					1146:1154	both the absolute and relative abundance	1115:1154	abundance	1146:1154	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	4	18	theme	Bacteroidaceae	687:700	arg1	species					702:708	naturally occurring Bacteroidaceae species	667:708	naturally occurring Bacteroidaceae species	667:708	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	4	19	from	study	765:769	arg1	mice					789:792	40 female NMRI mice	774:792	40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8)	774:944	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	7	20	theme	3FL	1583:1585	arg1	group					1587:1591	the 3FL group	1579:1591	the 3FL group	1579:1591	During a 1-week washout period performed specifically for the 3FL group, this effect was reversed.
37223362	1	21	theme	future	338:343	arg1	health					345:350	future health	338:350	future health	338:350	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	8	22	theme	acetate	1691:1697	arg1	levels					1725:1730	acetate, butyrate and isobutyrate levels	1691:1730	acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus	1691:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	0	23	attach	linked	121:126	arg1	reduction					133:141	a reduction	131:141	a reduction of butyrate levels	131:160	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	0	23	attach	linked	121:126	arg2	microbiota					89:98	the gut microbiota	81:98	the gut microbiota of conventional mice linked to a reduction of butyrate levels	81:160	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	5	24	theme	faecal	1185:1190	arg1	samples					1192:1198	faecal samples	1185:1198	faecal samples	1185:1198	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	2	25	theme	HMO	387:389	arg1	degradation					391:401	HMO degradation	387:401	HMO degradation	387:401	Bifidobacteria are specialized in HMO degradation and frequently dominate the gut microbiota of breastfed infants.
37223362	0	26	theme	conventional	103:114	arg1	mice					116:119	conventional mice	103:119	conventional mice	103:119	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	8	27	theme	butyrate	1700:1707	arg1	levels					1725:1730	acetate, butyrate and isobutyrate levels	1691:1730	acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus	1691:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	9	28	from	selection	1881:1889	arg1	environment					1902:1912	the gut environment	1894:1912	the gut environment	1894:1912	This study highlights HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia.
37223362	4	29	theme	gut	733:735	arg1	environment					737:747	a complex mammalian gut environment	713:747	a complex mammalian gut environment	713:747	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	0	30	theme	Human	0:4	arg1	oligosaccharides					11:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	6	31	theme	formerly	1485:1492	arg1	cluster					1511:1517	formerly Clostridium XIVa cluster	1485:1517	formerly Clostridium XIVa cluster	1485:1517	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	6	31	theme	formerly	1485:1492	arg1	Lacrimispora					1471:1482	the genus Lacrimispora	1461:1482	the genus Lacrimispora (formerly Clostridium XIVa cluster)	1461:1518	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	7	32	theme	washout	1537:1543	arg1	period					1545:1550	a 1-week washout period	1528:1550	a 1-week washout period performed specifically for the 3FL group	1528:1591	During a 1-week washout period performed specifically for the 3FL group, this effect was reversed.
37223362	2	33	theme	gut	431:433	arg1	microbiota					435:444	the gut microbiota	427:444	the gut microbiota of breastfed infants	427:465	Bifidobacteria are specialized in HMO degradation and frequently dominate the gut microbiota of breastfed infants.
37223362	6	34	theme	compositional	1293:1305	arg1	differences					1307:1317	The compositional differences	1289:1317	The compositional differences	1289:1317	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	1	35	theme	infant	278:283	arg1	gut					285:287	the infant gut	274:287	the infant gut	274:287	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	1	36	with	glycans	202:208	arg1	properties					225:234	prebiotic properties	215:234	prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health	215:350	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	8	37	from	levels	1725:1730	arg1	animals					1735:1741	animals	1735:1741	animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus	1735:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	5	38	theme	species	1174:1180	arg1	abundance					1146:1154	both the absolute and relative abundance	1115:1154	abundance	1146:1154	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	0	39	theme	acute	35:39	arg1	changes					70:76	acute yet reversible compositional changes	35:76	acute yet reversible compositional changes	35:76	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	4	40	dep	sialyllactose	851:863	arg1	=					874:874	=	874:874	=	874:874	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	2	41	theme	infants	459:465	arg1	microbiota					435:444	the gut microbiota	427:444	the gut microbiota of breastfed infants	427:465	Bifidobacteria are specialized in HMO degradation and frequently dominate the gut microbiota of breastfed infants.
37223362	9	42	theme	butyrate-producing	1946:1963	arg1	clostridia					1965:1974	butyrate-producing clostridia	1946:1974	butyrate-producing clostridia	1946:1974	This study highlights HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia.
37223362	5	43	with	supplementation	1053:1067	arg1	each					1074:1077	each	1074:1077	each	1074:1077	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	5	43	with	supplementation	1053:1067	arg1	HMOs					1086:1089	the HMOs	1082:1089	the HMOs	1082:1089	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	0	44	theme	reversible	45:54	arg1	changes					70:76	acute yet reversible compositional changes	35:76	acute yet reversible compositional changes	35:76	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	5	45	theme	unsupplemented	1014:1027	arg1	n					1045:1045	n = 8	1045:1049	n = 8	1045:1049	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	5	45	theme	unsupplemented	1014:1027	arg1	water					1038:1042	unsupplemented drinking water	1014:1042	unsupplemented drinking water (n = 8)	1014:1050	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	5	46	theme	16s rRNA	1259:1266	arg1	sequencing					1277:1286	16s rRNA amplicon sequencing	1259:1286	16s rRNA amplicon sequencing	1259:1286	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	6	47	theme	relative	1364:1371	arg1	abundance					1373:1381	the relative abundance	1360:1381	the relative abundance of the genus Phocaeicola (formerly Bacteroides)	1360:1429	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	4	48	theme	NMRI	784:787	arg1	mice					789:792	40 female NMRI mice	774:792	40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8)	774:944	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	5	49	dep	abundance	1146:1154	arg1	both					1115:1118	both	1115:1118	both	1115:1118	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	4	50	dep	Lacto-N-Tetraose	916:931	arg1	=					941:941	=	941:941	=	941:941	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	5	51	theme	microbial	1225:1233	arg1	composition					1235:1245	the overall microbial composition	1213:1245	the overall microbial composition analyzed by 16s rRNA amplicon sequencing	1213:1286	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	3	52	theme	Bacteroidaceae	493:506	arg1	species					482:488	some species	477:488	some species of Bacteroidaceae	477:506	However, some species of Bacteroidaceae also degrade HMOs, which may prompt selection also of these species in the gut microbiota.
37223362	1	53	theme	microbial	251:259	arg1	selection					261:269	microbial selection	251:269	microbial selection	251:269	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	8	54	from	decrease	1797:1804	arg1	genus					1826:1830	the Lacrimispora genus	1809:1830	the Lacrimispora genus	1809:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	1	55	theme	Human	163:167	arg1	Oligosaccharides					174:189	Human Milk Oligosaccharides	163:189	Human Milk Oligosaccharides (HMOs)	163:196	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	1	55	theme	Human	163:167	arg1	HMOs					192:195	HMOs	192:195	HMOs	192:195	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	1	55	theme	Human	163:167	arg1	glycans					202:208	glycans	202:208	glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health	202:350	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	4	56	theme	different	826:834	arg1	HMOs					836:839	three structurally different HMOs	807:839	three structurally different HMOs	807:839	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	4	57	dep	3FL	898:900	arg1	n					903:903	n = 16	903:908	n = 16	903:908	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	6	58	theme	genus	1390:1394	arg1	Phocaeicola					1396:1406	the genus Phocaeicola	1386:1406	the genus Phocaeicola (formerly Bacteroides)	1386:1429	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	6	58	theme	genus	1390:1394	arg1	Bacteroides					1418:1428	formerly Bacteroides	1409:1428	formerly Bacteroides	1409:1428	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	8	59	theme	observed	1788:1795	arg1	decrease					1797:1804	the observed decrease	1784:1804	the observed decrease in the Lacrimispora genus	1784:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	0	60	theme	butyrate	146:153	arg1	levels					155:160	butyrate levels	146:160	butyrate levels	146:160	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	6	61	theme	genus	1465:1469	arg1	cluster					1511:1517	formerly Clostridium XIVa cluster	1485:1517	formerly Clostridium XIVa cluster	1485:1517	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	6	61	theme	genus	1465:1469	arg1	Lacrimispora					1471:1482	the genus Lacrimispora	1461:1482	the genus Lacrimispora (formerly Clostridium XIVa cluster)	1461:1518	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	4	62	theme	species	702:708	arg1	abundance					654:662	the abundance	650:662	the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment	650:747	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	8	63	theme	Lacrimispora	1813:1824	arg1	genus					1826:1830	the Lacrimispora genus	1809:1830	the Lacrimispora genus	1809:1830	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	8	64	theme	acid	1638:1641	arg1	analysis					1643:1650	Short-chain fatty acid analysis	1620:1650	Short-chain fatty acid analysis of faecal water	1620:1666	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	6	65	theme	concomitant	1437:1447	arg1	decrease					1449:1456	a concomitant decrease	1435:1456	a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster)	1435:1518	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	9	66	theme	Bacteroidaceae	1866:1879	arg1	selection					1881:1889	HMO-driven Bacteroidaceae selection	1855:1889	HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia	1855:1974	This study highlights HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia.
37223362	0	67	theme	gut	85:87	arg1	microbiota					89:98	the gut microbiota	81:98	the gut microbiota of conventional mice linked to a reduction of butyrate levels	81:160	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	1	68	theme	immune	315:320	arg1	development					322:332	immune development	315:332	immune development	315:332	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	8	69	theme	water	1662:1666	arg1	analysis					1643:1650	Short-chain fatty acid analysis	1620:1650	Short-chain fatty acid analysis of faecal water	1620:1666	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	6	70	from	decrease	1449:1456	arg1	abundance					1373:1381	the relative abundance	1360:1381	the relative abundance of the genus Phocaeicola (formerly Bacteroides)	1360:1429	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	4	71	theme	=	905:905	arg1	n					903:903	n = 16	903:908	n = 16	903:908	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	4	72	theme	occurring	677:685	arg1	species					702:708	naturally occurring Bacteroidaceae species	667:708	naturally occurring Bacteroidaceae species	667:708	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	5	73	theme	control	990:996	arg1	group					998:1002	a control group	988:1002	a control group receiving unsupplemented drinking water (n = 8)	988:1050	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	9	74	theme	gut	1898:1900	arg1	environment					1902:1912	the gut environment	1894:1912	the gut environment	1894:1912	This study highlights HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia.
37223362	0	75	theme	mice	116:119	arg1	microbiota					89:98	the gut microbiota	81:98	the gut microbiota of conventional mice linked to a reduction of butyrate levels	81:160	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	4	76	dep	=	941:941	arg1	LNT					934:936	LNT	934:936	LNT	934:936	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	4	77	dep	=	874:874	arg1	SL					868:869	6'SL	866:869	6'SL	866:869	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	0	78	theme	milk	6:9	arg1	oligosaccharides					11:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides	0:26	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	6	79	theme	Clostridium	1494:1504	arg1	cluster					1511:1517	formerly Clostridium XIVa cluster	1485:1517	formerly Clostridium XIVa cluster	1485:1517	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	6	79	theme	Clostridium	1494:1504	arg1	Lacrimispora					1471:1482	the genus Lacrimispora	1461:1482	the genus Lacrimispora (formerly Clostridium XIVa cluster)	1461:1518	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	4	80	theme	mammalian	723:731	arg1	environment					737:747	a complex mammalian gut environment	713:747	a complex mammalian gut environment	713:747	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	5	81	theme	Bacteroidaceae	1159:1172	arg1	species					1174:1180	Bacteroidaceae species	1159:1180	Bacteroidaceae species	1159:1180	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	5	82	theme	=	1047:1047	arg1	n					1045:1045	n = 8	1045:1049	n = 8	1045:1049	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	5	82	theme	=	1047:1047	arg1	water					1038:1042	unsupplemented drinking water	1014:1042	unsupplemented drinking water (n = 8)	1014:1050	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	7	83	theme	1-week	1530:1535	arg1	period					1545:1550	a 1-week washout period	1528:1550	a 1-week washout period performed specifically for the 3FL group	1528:1591	During a 1-week washout period performed specifically for the 3FL group, this effect was reversed.
37223362	0	84	theme	compositional	56:68	arg1	changes					70:76	acute yet reversible compositional changes	35:76	acute yet reversible compositional changes	35:76	Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.
37223362	2	85	theme	breastfed	449:457	arg1	infants					459:465	breastfed infants	449:465	breastfed infants	449:465	Bifidobacteria are specialized in HMO degradation and frequently dominate the gut microbiota of breastfed infants.
37223362	9	86	theme	clostridia	1965:1974	arg1	reduction					1933:1941	a reduction	1931:1941	a reduction of butyrate-producing clostridia	1931:1974	This study highlights HMO-driven Bacteroidaceae selection in the gut environment, which may cause a reduction of butyrate-producing clostridia.
37223362	5	87	theme	drinking	1029:1036	arg1	n					1045:1045	n = 8	1045:1049	n = 8	1045:1049	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	5	87	theme	drinking	1029:1036	arg1	water					1038:1042	unsupplemented drinking water	1014:1042	unsupplemented drinking water (n = 8)	1014:1050	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
37223362	6	88	theme	XIVa	1506:1509	arg1	cluster					1511:1517	formerly Clostridium XIVa cluster	1485:1517	formerly Clostridium XIVa cluster	1485:1517	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	6	88	theme	XIVa	1506:1509	arg1	Lacrimispora					1471:1482	the genus Lacrimispora	1461:1482	the genus Lacrimispora (formerly Clostridium XIVa cluster)	1461:1518	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	8	89	theme	fatty	1632:1636	arg1	acid					1638:1641	Short-chain fatty acid	1620:1641	Short-chain fatty acid analysis of faecal water	1620:1666	Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the Lacrimispora genus.
37223362	1	90	theme	prebiotic	215:223	arg1	properties					225:234	prebiotic properties	215:234	prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health	215:350	Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health.
37223362	6	91	from	increase	1348:1355	arg1	abundance					1373:1381	the relative abundance	1360:1381	the relative abundance of the genus Phocaeicola (formerly Bacteroides)	1360:1429	The compositional differences were mainly attributed to an increase in the relative abundance of the genus Phocaeicola (formerly Bacteroides) and a concomitant decrease of the genus Lacrimispora (formerly Clostridium XIVa cluster).
37223362	4	92	theme	female	777:782	arg1	mice					789:792	40 female NMRI mice	774:792	40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8)	774:944	To investigate to what extent specific HMOs affect the abundance of naturally occurring Bacteroidaceae species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, n = 8), 3-fucosyllactose (3FL, n = 16), and Lacto-N-Tetraose (LNT, n = 8), through drinking water (5%).
37223362	5	93	theme	overall	1217:1223	arg1	composition					1235:1245	the overall microbial composition	1213:1245	the overall microbial composition analyzed by 16s rRNA amplicon sequencing	1213:1286	Compared to a control group receiving unsupplemented drinking water (n = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of Bacteroidaceae species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing.
36973252	8	0	theme	lower	1064:1068	arg1	levels					1070:1075	lower levels	1064:1075	lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals	1064:1200	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	8	1	dep	tissues	1094:1100	arg1	appears					1165:1171	appears	1165:1171	appears normal in Gpatch2-/- animals	1165:1200	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	6	2	from	mice	674:677	arg1	background					692:701	a C57BL/6 background	682:701	a C57BL/6 background	682:701	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	4	3	theme	cancer-testis	519:531	arg1	antigen					533:539	a proposed cancer-testis antigen	508:539	a proposed cancer-testis antigen with roles reported in proliferation in cell lines	508:590	GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines.
36973252	4	3	theme	cancer-testis	519:531	arg1	GPATCH2					497:503	GPATCH2	497:503	GPATCH2	497:503	GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines.
36973252	10	4	theme	GPATCH2	1530:1536	arg1	role					1522:1525	the role	1518:1525	the role of GPATCH2	1518:1536	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	7	5	theme	basal	877:881	arg1	expression					887:896	neither basal Tnf expression	869:896	neither basal Tnf expression in mice	869:904	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	3	6	theme	CRISPR	454:459	arg1	screen					461:466	a CRISPR screen	452:466	a CRISPR screen for novel regulators of TNF	452:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	4	7	theme	proposed	510:517	arg1	antigen					533:539	a proposed cancer-testis antigen	508:539	a proposed cancer-testis antigen with roles reported in proliferation in cell lines	508:590	GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines.
36973252	4	7	theme	proposed	510:517	arg1	GPATCH2					497:503	GPATCH2	497:503	GPATCH2	497:503	GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines.
36973252	10	8	theme	phenotype	1455:1463	arg1	absence					1435:1441	the absence	1431:1441	the absence of an overt phenotype in Gpatch2-/- mice	1431:1482	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	9	9	theme	Gpatch2-/-	1203:1212	arg1	mice					1214:1217	Gpatch2-/- mice	1203:1217	Gpatch2-/- mice	1203:1217	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	2	10	theme	system	308:313	arg1	normal					275:280	normal	275:280	normal	275:280	The control of TNF levels is therefore critical for normal functioning of the immune system and health.
36973252	7	11	theme	Tnf	883:885	arg1	expression					887:896	neither basal Tnf expression	869:896	neither basal Tnf expression in mice	869:904	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	7	12	theme	first	792:796	arg1	insights					798:805	the first insights	788:805	the first insights into Gpatch2-/- animals	788:829	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	10	13	theme	overt	1449:1453	arg1	phenotype					1455:1463	an overt phenotype	1446:1463	an overt phenotype	1446:1463	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	0	14	from	expression	39:48	arg1	mice					53:56	mice	53:56	mice	53:56	Deletion of Gpatch2 does not alter Tnf expression in mice.
36973252	2	15	theme	levels	242:247	arg1	critical					262:269	critical	262:269	critical	262:269	The control of TNF levels is therefore critical for normal functioning of the immune system and health.
36973252	2	15	theme	levels	242:247	arg1	control					227:233	The control	223:233	The control of TNF levels	223:247	The control of TNF levels is therefore critical for normal functioning of the immune system and health.
36973252	6	16	theme	GPATCH2	730:736	arg1	regulator					743:751	a regulator	741:751	a regulator of Tnf expression	741:769	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	6	16	theme	GPATCH2	730:736	arg1	potential					717:725	the potential	713:725	the potential of GPATCH2	713:736	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	3	17	theme	TNF	492:494	arg1	regulators					478:487	novel regulators	472:487	novel regulators of TNF	472:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	5	18	from	role	606:609	arg1	vivo					614:617	vivo	614:617	vivo	614:617	However, its role in vivo has not been established.
36973252	9	19	theme	blood	1319:1323	arg1	composition					1330:1340	blood cell composition	1319:1340	blood cell composition	1319:1340	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	8	20	theme	other	1088:1092	arg1	tissues					1094:1100	several other tissues	1080:1100	several other tissues	1080:1100	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	8	21	theme	tissues	1157:1163	arg1	morphology					1116:1125	the morphology	1112:1125	the morphology of the testis and these other tissues	1112:1163	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	2	22	theme	TNF	238:240	arg1	levels					242:247	TNF levels	238:247	TNF levels	238:247	The control of TNF levels is therefore critical for normal functioning of the immune system and health.
36973252	8	23	from	normal	1173:1178	arg1	animals					1194:1200	Gpatch2-/- animals	1183:1200	Gpatch2-/- animals	1183:1200	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	3	24	theme	expression	385:394	arg1	GPATCH2					346:352	GPATCH2	346:352	GPATCH2	346:352	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	3	24	theme	expression	385:394	arg1	repressor					368:376	a putative repressor	357:376	a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF	357:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	8	25	theme	several	1080:1086	arg1	tissues					1094:1100	several other tissues	1080:1100	several other tissues	1080:1100	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	9	26	theme	lymphoid	1299:1306	arg1	tissues					1308:1314	lymphoid tissues	1299:1314	lymphoid tissues	1299:1314	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	8	27	dep	testis	1134:1139	arg1	the					1130:1132	the	1130:1132	the	1130:1132	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	8	28	theme	testis	1134:1139	arg1	morphology					1116:1125	the morphology	1112:1125	the morphology of the testis and these other tissues	1112:1163	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	7	29	theme	inflammation	999:1010	arg1	models					989:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models	929:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation	929:1010	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	7	30	theme	GPATCH2	853:859	arg1	loss					845:848	loss	845:848	loss of GPATCH2	845:859	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	1	31	theme	cytokine	63:70	arg1	TNF					72:74	The cytokine TNF	59:74	The cytokine TNF	59:74	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
36973252	8	32	theme	GPATCH2	1025:1031	arg1	protein					1033:1039	GPATCH2 protein	1025:1039	GPATCH2 protein	1025:1039	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	8	33	theme	other	1151:1155	arg1	tissues					1157:1163	these other tissues	1145:1163	these other tissues	1145:1163	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	10	34	theme	discernible	1380:1390	arg1	role					1392:1395	no discernible role	1377:1395	no discernible role of GPATCH2 in Tnf expression	1377:1424	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	10	35	theme	further	1493:1499	arg1	investigation					1501:1513	further investigation	1493:1513	further investigation of the role of GPATCH2	1493:1536	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	3	36	from	UTR	445:447	arg1	screen					461:466	a CRISPR screen	452:466	a CRISPR screen for novel regulators of TNF	452:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	3	37	theme	Tnf	381:383	arg1	expression					385:394	Tnf expression	381:394	Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF	381:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	10	38	theme	Gpatch2-/-	1468:1477	arg1	mice					1479:1482	Gpatch2-/- mice	1468:1482	Gpatch2-/- mice	1468:1482	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	8	39	from	animals	1194:1200	arg1	normal					1173:1178	normal	1173:1178	normal	1173:1178	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	9	40	theme	cell	1325:1328	arg1	composition					1330:1340	blood cell composition	1319:1340	blood cell composition	1319:1340	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	4	41	theme	cell	581:584	arg1	lines					586:590	cell lines	581:590	cell lines	581:590	GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines.
36973252	0	42	theme	Gpatch2	12:18	arg1	Deletion					0:7	Deletion	0:7	Deletion of Gpatch2	0:18	Deletion of Gpatch2 does not alter Tnf expression in mice.
36973252	10	43	theme	GPATCH2	1400:1406	arg1	role					1392:1395	no discernible role	1377:1395	no discernible role of GPATCH2 in Tnf expression	1377:1424	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	3	44	theme	novel	472:476	arg1	regulators					478:487	novel regulators	472:487	novel regulators of TNF	472:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	1	45	theme	essential	80:88	arg1	roles					90:94	essential roles	80:94	essential roles	80:94	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
36973252	3	46	theme	TNF	438:440	arg1	UTR					445:447	the TNF 3' UTR	434:447	the TNF 3' UTR in a CRISPR screen for novel regulators of TNF	434:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	3	47	theme	putative	359:366	arg1	GPATCH2					346:352	GPATCH2	346:352	GPATCH2	346:352	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	3	47	theme	putative	359:366	arg1	repressor					368:376	a putative repressor	357:376	a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF	357:494	We have identified GPATCH2 as a putative repressor of Tnf expression acting post-transcriptionally through the TNF 3' UTR in a CRISPR screen for novel regulators of TNF.
36973252	7	48	theme	SMAC-mimetic	966:977	arg1	injection					979:987	intraperitoneal LPS and subcutaneous SMAC-mimetic injection	929:987	injection	979:987	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	10	49	theme	role	1522:1525	arg1	investigation					1501:1513	further investigation	1493:1513	further investigation of the role of GPATCH2	1493:1536	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	7	50	from	expression	915:924	arg1	models					989:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models	929:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation	929:1010	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	7	51	from	expression	887:896	arg1	mice					901:904	mice	901:904	mice	901:904	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	9	52	dep	viable	1223:1228	arg1	appear					1231:1236	appear	1231:1236	appear grossly normal	1231:1251	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	7	53	theme	injection	979:987	arg1	models					989:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models	929:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation	929:1010	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	10	54	from	absence	1435:1441	arg1	mice					1479:1482	Gpatch2-/- mice	1468:1482	Gpatch2-/- mice	1468:1482	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	1	55	contain	has	76:78	arg1	TNF					72:74	The cytokine TNF	59:74	The cytokine TNF	59:74	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
36973252	1	55	contain	has	76:78	arg2	roles					90:94	essential roles	80:94	essential roles	80:94	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
36973252	9	56	from	aberrations	1284:1294	arg1	tissues					1308:1314	lymphoid tissues	1299:1314	lymphoid tissues	1299:1314	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	9	56	from	aberrations	1284:1294	arg1	composition					1330:1340	blood cell composition	1319:1340	blood cell composition	1319:1340	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	6	57	theme	C57BL/6	684:690	arg1	background					692:701	a C57BL/6 background	682:701	a C57BL/6 background	682:701	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	2	58	theme	immune	301:306	arg1	system					308:313	the immune system	297:313	the immune system	297:313	The control of TNF levels is therefore critical for normal functioning of the immune system and health.
36973252	0	59	theme	Tnf	35:37	arg1	expression					39:48	Tnf expression	35:48	Tnf expression in mice	35:56	Deletion of Gpatch2 does not alter Tnf expression in mice.
36973252	7	60	theme	subcutaneous	953:964	arg1	injection					979:987	intraperitoneal LPS and subcutaneous SMAC-mimetic injection	929:987	injection	979:987	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	6	61	theme	expression	760:769	arg1	regulator					743:751	a regulator	741:751	a regulator of Tnf expression	741:769	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	6	61	theme	expression	760:769	arg1	potential					717:725	the potential	713:725	the potential of GPATCH2	713:736	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	8	62	theme	Gpatch2-/-	1183:1192	arg1	animals					1194:1200	Gpatch2-/- animals	1183:1200	Gpatch2-/- animals	1183:1200	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	7	63	theme	intraperitoneal	929:943	arg1	LPS					945:947	intraperitoneal LPS and subcutaneous SMAC-mimetic injection	929:987	LPS	945:947	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	6	64	theme	Tnf	756:758	arg1	expression					760:769	Tnf expression	756:769	Tnf expression	756:769	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	7	65	theme	Tnf	911:913	arg1	expression					915:924	Tnf expression	911:924	Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation	911:1010	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	1	66	theme	immune	99:104	arg1	defence					106:112	immune defence	99:112	immune defence against diverse pathogens	99:138	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
36973252	7	67	theme	LPS	945:947	arg1	models					989:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models	929:994	intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation	929:1010	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	4	68	with	antigen	533:539	arg1	roles					546:550	roles	546:550	roles reported in proliferation in cell lines	546:590	GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines.
36973252	8	69	theme	mouse	1044:1048	arg1	testis					1050:1055	mouse testis	1044:1055	mouse testis	1044:1055	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	1	70	theme	diverse	122:128	arg1	pathogens					130:138	diverse pathogens	122:138	diverse pathogens	122:138	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
36973252	7	71	theme	Gpatch2-/-	812:821	arg1	animals					823:829	Gpatch2-/- animals	812:829	Gpatch2-/- animals	812:829	Here we provide the first insights into Gpatch2-/- animals and show that loss of GPATCH2 affects neither basal Tnf expression in mice, nor Tnf expression in intraperitoneal LPS and subcutaneous SMAC-mimetic injection models of inflammation.
36973252	9	72	theme	notable	1276:1282	arg1	aberrations					1284:1294	notable aberrations	1276:1294	notable aberrations in lymphoid tissues or blood cell composition	1276:1340	Gpatch2-/- mice are viable, appear grossly normal, and we did not detect notable aberrations in lymphoid tissues or blood cell composition.
36973252	4	73	from	proliferation	564:576	arg1	lines					586:590	cell lines	581:590	cell lines	581:590	GPATCH2 is a proposed cancer-testis antigen with roles reported in proliferation in cell lines.
36973252	8	74	from	levels	1070:1075	arg1	tissues					1094:1100	several other tissues	1080:1100	several other tissues	1080:1100	We detected GPATCH2 protein in mouse testis and at lower levels in several other tissues, however, the morphology of the testis and these other tissues appears normal in Gpatch2-/- animals.
36973252	6	75	theme	Gpatch2-/-	663:672	arg1	mice					674:677	Gpatch2-/- mice	663:677	Gpatch2-/- mice on a C57BL/6 background	663:701	We have generated Gpatch2-/- mice on a C57BL/6 background to assess the potential of GPATCH2 as a regulator of Tnf expression.
36973252	10	76	theme	Tnf	1411:1413	arg1	expression					1415:1424	Tnf expression	1411:1424	Tnf expression	1411:1424	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	1	77	theme	severe	194:199	arg1	disease					214:220	severe inflammatory disease	194:220	severe inflammatory disease	194:220	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
36973252	10	78	from	role	1392:1395	arg1	expression					1415:1424	Tnf expression	1411:1424	Tnf expression	1411:1424	Collectively, our results suggest no discernible role of GPATCH2 in Tnf expression, and the absence of an overt phenotype in Gpatch2-/- mice warrants further investigation of the role of GPATCH2.
36973252	2	79	theme	health	319:324	arg1	normal					275:280	normal	275:280	normal	275:280	The control of TNF levels is therefore critical for normal functioning of the immune system and health.
36973252	1	80	theme	inflammatory	201:212	arg1	disease					214:220	severe inflammatory disease	194:220	severe inflammatory disease	194:220	The cytokine TNF has essential roles in immune defence against diverse pathogens and, when its expression is deregulated, it can drive severe inflammatory disease.
37353115	12	0	theme	anti-HCV-specific	2206:2222	arg1	antibody					2224:2231	an anti-HCV-specific antibody	2203:2231	an anti-HCV-specific antibody	2203:2231	Evidence for persistent virus production was monitored by the ELISA technique using an anti-HCV-specific antibody.
37353115	10	1	theme	angustifalia	1551:1562	arg1	extract					1564:1570	Boscia angustifalia extract	1544:1570	Boscia angustifalia extract	1544:1570	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	3	2	theme	recurrence	684:693	arg1	chance					652:657	a 0.5 % chance	644:657	a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections	644:736	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	1	3	theme	liver	252:256	arg1	carcinoma					332:340	hepatocellular carcinoma	317:340	hepatocellular carcinoma	317:340	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	3	theme	liver	252:256	arg1	diseases					258:265	chronic liver diseases	244:265	chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide	244:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	3	theme	liver	252:256	arg1	hepatitis					285:293	chronic hepatitis	277:293	chronic hepatitis	277:293	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	3	theme	liver	252:256	arg1	cirrhosis					302:310	liver cirrhosis	296:310	liver cirrhosis	296:310	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	10	4	theme	46.58 ± 1.33 	1588:1600	arg1	percentage					1513:1522	The percentage	1509:1522	The percentage of encapsulation of Boscia angustifalia extract	1509:1570	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	10	4	theme	46.58 ± 1.33 	1588:1600	arg1	%					1601:1601	%	1601:1601	%	1601:1601	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	3	5	theme	viral	721:725	arg1	infections					727:736	other chronic viral infections	707:736	other chronic viral infections	707:736	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	3	6	theme	hepatitis	567:575	arg1	C					577:577	chronic hepatitis C	559:577	chronic hepatitis C	559:577	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	8	7	theme	l-ascorbic	1184:1193	arg1	reagents					1216:1223	l-ascorbic instead of hazardous reagents	1184:1223	l-ascorbic instead of hazardous reagents	1184:1223	In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations.
37353115	13	8	theme	anti-HCV	2282:2289	arg1	activity					2291:2298	an anti-HCV activity	2279:2298	an anti-HCV activity that increased by increasing concentration	2279:2341	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	3	9	theme	other	707:711	arg1	infections					727:736	other chronic viral infections	707:736	other chronic viral infections	707:736	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	11	10	theme	cells	2112:2116	arg1	infection					2084:2092	infection	2084:2092	infection of human hepatoma cells	2084:2116	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	1	11	theme	C	200:200	arg1	virus					202:206	Hepatitis C virus	190:206	Hepatitis C virus (HCV)	190:212	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	11	theme	C	200:200	arg1	agent					235:239	a major causative agent	217:239	a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide	217:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	11	theme	C	200:200	arg1	HCV					209:211	HCV	209:211	HCV	209:211	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	8	12	theme	tested	1258:1263	arg1	extracts					1276:1283	two Boscia extracts	1265:1283	newly tested two Boscia extracts each	1252:1288	In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations.
37353115	10	13	theme	Boscia	1611:1616	arg1	extract					1631:1637	Boscia senegalensis extract	1611:1637	Boscia senegalensis extract	1611:1637	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	8	14	theme	Boscia	1269:1274	arg1	extracts					1276:1283	two Boscia extracts	1265:1283	newly tested two Boscia extracts each	1252:1288	In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations.
37353115	13	15	theme	remarkable	2372:2381	arg1	activity					2392:2399	remarkable anti-HCV activity	2372:2399	remarkable anti-HCV activity	2372:2399	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	7	16	theme	HCV	1089:1091	arg1	treatment					1093:1101	HCV treatment	1089:1101	HCV treatment	1089:1101	Galactosylated chitosan nanoparticles have been widely applied in HCV treatment.
37353115	6	17	theme	well-known	947:956	arg1	nanoparticles					929:941	chitosan nanoparticles	920:941	chitosan nanoparticles	920:941	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	17	theme	well-known	947:956	arg1	carriers					1013:1020	polymeric carriers	1003:1020	polymeric carriers	1003:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	17	theme	well-known	947:956	arg1	systems					967:973	well-known delivery systems	947:973	well-known delivery systems that are frequently used as polymeric carriers	947:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	11	18	from	%	1782:1782	arg1	pH.					1794:1796	normal pH.	1787:1796	normal pH.	1787:1796	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	0	19	theme	eco-friendly	151:162	arg1	method					164:169	eco-friendly method	151:169	eco-friendly method for HCV treatment	151:187	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	11	20	theme	C	2045:2045	arg1	HCV					2054:2056	HCV	2054:2056	HCV	2054:2056	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	20	theme	C	2045:2045	arg1	virus					2047:2051	hepatitis C virus	2035:2051	hepatitis C virus (HCV) which was established by infection of human hepatoma cells	2035:2116	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	3	21	theme	direct-acting	608:620	arg1	antivirals					622:631	direct-acting antivirals	608:631	direct-acting antivirals (DAA)	608:637	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	3	21	theme	direct-acting	608:620	arg1	DAA					634:636	DAA	634:636	DAA	634:636	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	11	22	from	180 min	1761:1767	arg1	pH.					1794:1796	normal pH.	1787:1796	normal pH.	1787:1796	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	9	23	theme	zeta	1435:1438	arg1	potential					1440:1448	a zeta potential	1433:1448	a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV	1433:1506	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	0	24	theme	&Boscia	58:64	arg1	senegalensis					66:77	Boscia angustifalia &Boscia senegalensis	38:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis	0:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	3	25	with	therapy	595:601	arg1	antivirals					622:631	direct-acting antivirals	608:631	direct-acting antivirals (DAA)	608:637	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	3	25	with	therapy	595:601	arg1	DAA					634:636	DAA	634:636	DAA	634:636	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	6	26	theme	polymeric	1003:1011	arg1	carriers					1013:1020	polymeric carriers	1003:1020	polymeric carriers	1003:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	26	theme	polymeric	1003:1011	arg1	systems					967:973	well-known delivery systems	947:973	well-known delivery systems that are frequently used as polymeric carriers	947:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	11	27	theme	extract	1693:1699	arg1	release					1662:1668	The release	1658:1668	The release of Boscia angustifalia extract from the nanoparticles	1658:1722	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	27	theme	extract	1693:1699	arg1	%					1737:1737	about 40 %	1728:1737	about 40 %	1728:1737	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	28	dep	%	1782:1782	arg1	%					1882:1882	56 %	1879:1882	56 % in normal media	1879:1898	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	28	dep	%	1782:1782	arg1	release					1811:1817	the release	1807:1817	the release of Boscia senegalensis extract	1807:1848	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	28	dep	%	1782:1782	arg1	%					1857:1857	20 %	1854:1857	20 % in acidic media	1854:1873	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	1	29	dep	hepatitis	285:293	arg1	worldwide					342:350	worldwide	342:350	worldwide	342:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	8	30	dep	extracts	1276:1283	arg1	each					1285:1288	each	1285:1288	each	1285:1288	In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations.
37353115	2	31	theme	current	423:429	arg1	regimens					440:447	the current all-oral regimens	419:447	the current all-oral regimens using direct-acting antivirals	419:478	Treatment of HCV has evolved from early interferon monotherapy to the current all-oral regimens using direct-acting antivirals.
37353115	0	32	theme	chitosan	113:120	arg1	nanoparticles					122:134	galactosylated chitosan nanoparticles	98:134	galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment	98:187	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	9	33	theme	synthesized	1329:1339	arg1	nanoparticles					1350:1362	The synthesized chitosan nanoparticles	1325:1362	The synthesized chitosan nanoparticles	1325:1362	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	11	34	theme	hepatoma	2103:2110	arg1	cells					2112:2116	human hepatoma cells	2097:2116	human hepatoma cells	2097:2116	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	9	35	theme	dispersion	1375:1384	arg1	peaks					1386:1390	two dispersion peaks	1371:1390	two dispersion peaks	1371:1390	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	2	36	theme	early	387:391	arg1	monotherapy					404:414	early interferon monotherapy	387:414	early interferon monotherapy to the current all-oral regimens using direct-acting antivirals	387:478	Treatment of HCV has evolved from early interferon monotherapy to the current all-oral regimens using direct-acting antivirals.
37353115	1	37	theme	chronic	277:283	arg1	hepatitis					285:293	chronic hepatitis	277:293	chronic hepatitis	277:293	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	0	38	dep	extracts	79:86	arg1	loaded					88:93	loaded	88:93	extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment	79:187	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	4	39	theme	retreatment	743:753	arg1	options					755:761	retreatment options	743:761	retreatment options following treatment failure	743:789	So, retreatment options following treatment failure have become crucial issues.
37353115	2	40	theme	all-oral	431:438	arg1	regimens					440:447	the current all-oral regimens	419:447	the current all-oral regimens using direct-acting antivirals	419:478	Treatment of HCV has evolved from early interferon monotherapy to the current all-oral regimens using direct-acting antivirals.
37353115	0	41	theme	novel	17:21	arg1	therapies					27:35	novel HCV therapies	17:35	novel HCV therapies	17:35	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	11	42	theme	normal	1887:1892	arg1	media					1894:1898	normal media	1887:1898	normal media	1887:1898	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	9	43	theme	+3.3 ± 0.4mV	1453:1464	arg1	potential					1440:1448	a zeta potential	1433:1448	a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV	1433:1506	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	0	44	theme	therapies	27:35	arg1	Investigation					0:12	Investigation	0:12	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis	0:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	10	45	theme	Boscia	1544:1549	arg1	extract					1564:1570	Boscia angustifalia extract	1544:1570	Boscia angustifalia extract	1544:1570	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	3	46	theme	%	650:650	arg1	chance					652:657	a 0.5 % chance	644:657	a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections	644:736	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	12	47	theme	persistent	2132:2141	arg1	production					2149:2158	persistent virus production	2132:2158	persistent virus production	2132:2158	Evidence for persistent virus production was monitored by the ELISA technique using an anti-HCV-specific antibody.
37353115	11	48	theme	Boscia	1673:1678	arg1	extract					1693:1699	Boscia angustifalia extract	1673:1699	Boscia angustifalia extract	1673:1699	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	0	49	theme	Boscia	38:43	arg1	senegalensis					66:77	Boscia angustifalia &Boscia senegalensis	38:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis	0:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	11	50	theme	developed	1939:1947	arg1	composites					1949:1958	these two newly developed composites	1923:1958	these two newly developed composites	1923:1958	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	9	51	with	+3.3 ± 0.4mV	1453:1464	arg1	stability					1476:1484	high stability	1471:1484	high stability in a range of 40.7 mV	1471:1506	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	3	52	theme	virus	678:682	arg1	recurrence					684:693	the hepatitis C virus recurrence	662:693	the hepatitis C virus recurrence	662:693	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	11	53	theme	senegalensis	1829:1840	arg1	extract					1842:1848	Boscia senegalensis extract	1822:1848	Boscia senegalensis extract	1822:1848	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	1	54	theme	major	219:223	arg1	agent					235:239	a major causative agent	217:239	a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide	217:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	54	theme	major	219:223	arg1	virus					202:206	Hepatitis C virus	190:206	Hepatitis C virus (HCV)	190:212	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	3	55	theme	hepatitis	666:674	arg1	virus					678:682	the hepatitis C virus	662:682	the hepatitis C virus recurrence	662:693	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	11	56	theme	acidic	1862:1867	arg1	media					1869:1873	acidic media	1862:1873	acidic media	1862:1873	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	5	57	theme	new	859:861	arg1	therapy					873:879	a new promising therapy	857:879	a new promising therapy for HCV	857:887	Hence, this study aims to investigate a new promising therapy for HCV.
37353115	6	58	theme	nanomedicine	906:917	arg1	field					897:901	the field	893:901	the field of nanomedicine	893:917	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	0	59	dep	Investigation	0:12	arg1	senegalensis					66:77	Boscia angustifalia &Boscia senegalensis	38:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis	0:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	10	60	theme	extract	1564:1570	arg1	encapsulation					1527:1539	encapsulation	1527:1539	encapsulation of Boscia angustifalia extract	1527:1570	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	11	61	theme	angustifalia	1680:1691	arg1	extract					1693:1699	Boscia angustifalia extract	1673:1699	Boscia angustifalia extract	1673:1699	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	2	62	theme	HCV	366:368	arg1	Treatment					353:361	Treatment	353:361	Treatment of HCV	353:368	Treatment of HCV has evolved from early interferon monotherapy to the current all-oral regimens using direct-acting antivirals.
37353115	1	63	theme	chronic	244:250	arg1	carcinoma					332:340	hepatocellular carcinoma	317:340	hepatocellular carcinoma	317:340	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	63	theme	chronic	244:250	arg1	diseases					258:265	chronic liver diseases	244:265	chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide	244:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	63	theme	chronic	244:250	arg1	hepatitis					285:293	chronic hepatitis	277:293	chronic hepatitis	277:293	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	63	theme	chronic	244:250	arg1	cirrhosis					302:310	liver cirrhosis	296:310	liver cirrhosis	296:310	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	6	64	used	used	995:998	arg2	carriers					1013:1020	polymeric carriers	1003:1020	polymeric carriers	1003:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	64	used	used	995:998	arg2	systems					967:973	well-known delivery systems	947:973	well-known delivery systems that are frequently used as polymeric carriers	947:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	64	used	used	995:998	arg2	nanoparticles					929:941	chitosan nanoparticles	920:941	chitosan nanoparticles	920:941	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	3	65	theme	chronic	559:565	arg1	C					577:577	chronic hepatitis C	559:577	chronic hepatitis C	559:577	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	1	66	theme	diseases	258:265	arg1	agent					235:239	a major causative agent	217:239	a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide	217:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	66	theme	diseases	258:265	arg1	virus					202:206	Hepatitis C virus	190:206	Hepatitis C virus (HCV)	190:212	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	8	67	theme	eco-friendly	1158:1169	arg1	method					1171:1176	an eco-friendly method	1155:1176	an eco-friendly method using l-ascorbic instead of hazardous reagents	1155:1223	In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations.
37353115	9	68	theme	high	1471:1474	arg1	stability					1476:1484	high stability	1471:1484	high stability in a range of 40.7 mV	1471:1506	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	1	69	theme	Hepatitis	190:198	arg1	virus					202:206	Hepatitis C virus	190:206	Hepatitis C virus (HCV)	190:212	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	69	theme	Hepatitis	190:198	arg1	agent					235:239	a major causative agent	217:239	a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide	217:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	69	theme	Hepatitis	190:198	arg1	HCV					209:211	HCV	209:211	HCV	209:211	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	10	70	theme	senegalensis	1618:1629	arg1	extract					1631:1637	Boscia senegalensis extract	1611:1637	Boscia senegalensis extract	1611:1637	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	3	71	theme	chronic	713:719	arg1	infections					727:736	other chronic viral infections	707:736	other chronic viral infections	707:736	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	11	72	theme	in	1997:1998	arg1	system					2006:2011	an in vitro system	1994:2011	an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells	1994:2116	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	5	73	theme	promising	863:871	arg1	therapy					873:879	a new promising therapy	857:879	a new promising therapy for HCV	857:887	Hence, this study aims to investigate a new promising therapy for HCV.
37353115	3	74	theme	C	577:577	arg1	treatment					546:554	the treatment	542:554	the treatment of chronic hepatitis C	542:577	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	8	75	theme	hazardous	1206:1214	arg1	reagents					1216:1223	l-ascorbic instead of hazardous reagents	1184:1223	l-ascorbic instead of hazardous reagents	1184:1223	In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations.
37353115	6	76	theme	delivery	958:965	arg1	nanoparticles					929:941	chitosan nanoparticles	920:941	chitosan nanoparticles	920:941	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	76	theme	delivery	958:965	arg1	carriers					1013:1020	polymeric carriers	1003:1020	polymeric carriers	1003:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	76	theme	delivery	958:965	arg1	systems					967:973	well-known delivery systems	947:973	well-known delivery systems that are frequently used as polymeric carriers	947:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	3	77	theme	critical	524:531	arg1	issue					533:537	a critical issue	522:537	a critical issue	522:537	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	11	78	theme	Boscia	1822:1827	arg1	extract					1842:1848	Boscia senegalensis extract	1822:1848	Boscia senegalensis extract	1822:1848	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	0	79	theme	HCV	175:177	arg1	treatment					179:187	HCV treatment	175:187	HCV treatment	175:187	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	7	80	theme	Galactosylated	1023:1036	arg1	nanoparticles					1047:1059	Galactosylated chitosan nanoparticles	1023:1059	Galactosylated chitosan nanoparticles	1023:1059	Galactosylated chitosan nanoparticles have been widely applied in HCV treatment.
37353115	9	81	theme	40.7 mV	1500:1506	arg1	range					1491:1495	a range	1489:1495	a range of 40.7 mV	1489:1506	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	13	82	contain	had	2368:2370	arg2	activity					2392:2399	remarkable anti-HCV activity	2372:2399	remarkable anti-HCV activity	2372:2399	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	13	82	contain	had	2368:2370	arg1	angustifalia					2355:2366	Boscia angustifalia	2348:2366	Boscia angustifalia	2348:2366	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	6	83	theme	chitosan	920:927	arg1	nanoparticles					929:941	chitosan nanoparticles	920:941	chitosan nanoparticles	920:941	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	6	83	theme	chitosan	920:927	arg1	systems					967:973	well-known delivery systems	947:973	well-known delivery systems that are frequently used as polymeric carriers	947:1020	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	11	84	theme	virus	2047:2051	arg1	production					2021:2030	the production	2017:2030	the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells	2017:2116	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	85	theme	acidic	1742:1747	arg1	media					1749:1753	acidic media	1742:1753	acidic media	1742:1753	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	7	86	theme	chitosan	1038:1045	arg1	nanoparticles					1047:1059	Galactosylated chitosan nanoparticles	1023:1059	Galactosylated chitosan nanoparticles	1023:1059	Galactosylated chitosan nanoparticles have been widely applied in HCV treatment.
37353115	11	87	theme	composites	1949:1958	arg1	Testing					1912:1918	24 h. Testing	1906:1918	24 h. Testing of these two newly developed composites against HCV	1906:1970	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	88	theme	hepatitis	2035:2043	arg1	HCV					2054:2056	HCV	2054:2056	HCV	2054:2056	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	88	theme	hepatitis	2035:2043	arg1	virus					2047:2051	hepatitis C virus	2035:2051	hepatitis C virus (HCV) which was established by infection of human hepatoma cells	2035:2116	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	8	89	theme	different	1299:1307	arg1	concentrations					1309:1322	three different concentrations	1293:1322	three different concentrations	1293:1322	In this study, we have modified galactosylation by an eco-friendly method using l-ascorbic instead of hazardous reagents and we have loaded it with newly tested two Boscia extracts each in three different concentrations.
37353115	10	90	theme	encapsulation	1527:1539	arg1	percentage					1513:1522	The percentage	1509:1522	The percentage of encapsulation of Boscia angustifalia extract	1509:1570	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	10	90	theme	encapsulation	1527:1539	arg1	%					1601:1601	%	1601:1601	%	1601:1601	The percentage of encapsulation of Boscia angustifalia extract was found to be 46.58 ± 1.33 % and for Boscia senegalensis extract was 9.77 ± 0.33 %.
37353115	11	91	from	%	1737:1737	arg1	media					1749:1753	acidic media	1742:1753	acidic media	1742:1753	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	92	theme	24 h.	1906:1910	arg1	Testing					1912:1918	24 h. Testing	1906:1918	24 h. Testing of these two newly developed composites against HCV	1906:1970	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	93	from	%	1882:1882	arg1	media					1894:1898	normal media	1887:1898	normal media	1887:1898	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	93	from	%	1882:1882	arg1	media					1869:1873	acidic media	1862:1873	acidic media	1862:1873	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	94	dep	in	1997:1998	arg1	vitro					2000:2004	vitro	2000:2004	vitro	2000:2004	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	95	from	%	1857:1857	arg1	media					1894:1898	normal media	1887:1898	normal media	1887:1898	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	95	from	%	1857:1857	arg1	media					1869:1873	acidic media	1862:1873	acidic media	1862:1873	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	0	96	theme	galactosylated	98:111	arg1	nanoparticles					122:134	galactosylated chitosan nanoparticles	98:134	galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment	98:187	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	11	97	from	nanoparticles	1710:1722	arg1	release					1662:1668	The release	1658:1668	The release of Boscia angustifalia extract from the nanoparticles	1658:1722	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	97	from	nanoparticles	1710:1722	arg1	%					1737:1737	about 40 %	1728:1737	about 40 %	1728:1737	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	13	98	theme	Boscia	2413:2418	arg1	senegalensis					2420:2431	Boscia senegalensis	2413:2431	Boscia senegalensis	2413:2431	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	4	99	theme	treatment	773:781	arg1	failure					783:789	treatment failure	773:789	treatment failure	773:789	So, retreatment options following treatment failure have become crucial issues.
37353115	2	100	theme	interferon	393:402	arg1	monotherapy					404:414	early interferon monotherapy	387:414	early interferon monotherapy to the current all-oral regimens using direct-acting antivirals	387:478	Treatment of HCV has evolved from early interferon monotherapy to the current all-oral regimens using direct-acting antivirals.
37353115	9	101	theme	chitosan	1341:1348	arg1	nanoparticles					1350:1362	The synthesized chitosan nanoparticles	1325:1362	The synthesized chitosan nanoparticles	1325:1362	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	2	102	theme	direct-acting	455:467	arg1	antivirals					469:478	direct-acting antivirals	455:478	direct-acting antivirals	455:478	Treatment of HCV has evolved from early interferon monotherapy to the current all-oral regimens using direct-acting antivirals.
37353115	12	103	theme	virus	2143:2147	arg1	production					2149:2158	persistent virus production	2132:2158	persistent virus production	2132:2158	Evidence for persistent virus production was monitored by the ELISA technique using an anti-HCV-specific antibody.
37353115	0	104	theme	HCV	23:25	arg1	therapies					27:35	novel HCV therapies	17:35	novel HCV therapies	17:35	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	13	105	theme	anti-HCV	2383:2390	arg1	activity					2392:2399	remarkable anti-HCV activity	2372:2399	remarkable anti-HCV activity	2372:2399	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	11	106	theme	human	2097:2101	arg1	cells					2112:2116	human hepatoma cells	2097:2116	human hepatoma cells	2097:2116	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	3	107	theme	antiviral	490:498	arg1	resistance					500:509	antiviral resistance	490:509	antiviral resistance	490:509	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	1	108	theme	liver	296:300	arg1	cirrhosis					302:310	liver cirrhosis	296:310	liver cirrhosis	296:310	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	6	109	from	systems	967:973	arg1	field					897:901	the field	893:901	the field of nanomedicine	893:917	In the field of nanomedicine, chitosan nanoparticles are well-known delivery systems that are frequently used as polymeric carriers.
37353115	3	110	theme	0.5 	646:649	arg1	%					650:650	%	650:650	%	650:650	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	0	111	theme	angustifalia	45:56	arg1	senegalensis					66:77	Boscia angustifalia &Boscia senegalensis	38:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis	0:77	Investigation of novel HCV therapies: Boscia angustifalia &Boscia senegalensis extracts loaded on galactosylated chitosan nanoparticles synthesized by eco-friendly method for HCV treatment.
37353115	13	112	contain	had	2275:2277	arg1	samples					2267:2273	all samples	2263:2273	all samples	2263:2273	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	13	112	contain	had	2275:2277	arg2	activity					2291:2298	an anti-HCV activity	2279:2298	an anti-HCV activity that increased by increasing concentration	2279:2341	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	13	113	theme	Boscia	2348:2353	arg1	angustifalia					2355:2366	Boscia angustifalia	2348:2366	Boscia angustifalia	2348:2366	Results obtained showed that all samples had an anti-HCV activity that increased by increasing concentration, and Boscia angustifalia had remarkable anti-HCV activity compared to Boscia senegalensis.
37353115	3	114	theme	C	676:676	arg1	virus					678:682	the hepatitis C virus	662:682	the hepatitis C virus recurrence	662:693	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	1	115	theme	hepatocellular	317:330	arg1	carcinoma					332:340	hepatocellular carcinoma	317:340	hepatocellular carcinoma	317:340	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	12	116	theme	ELISA	2181:2185	arg1	technique					2187:2195	the ELISA technique	2177:2195	the ELISA technique using an anti-HCV-specific antibody	2177:2231	Evidence for persistent virus production was monitored by the ELISA technique using an anti-HCV-specific antibody.
37353115	11	117	theme	extract	1842:1848	arg1	release					1811:1817	the release	1807:1817	the release of Boscia senegalensis extract	1807:1848	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	11	117	theme	extract	1842:1848	arg1	%					1857:1857	20 %	1854:1857	20 % in acidic media	1854:1873	The release of Boscia angustifalia extract from the nanoparticles was about 40 % in acidic media after 180 min and about 60 % in normal pH. However, the release of Boscia senegalensis extract was 20 % in acidic media and 56 % in normal media after 24 h. Testing of these two newly developed composites against HCV was carried out using an in vitro system for the production of hepatitis C virus (HCV) which was established by infection of human hepatoma cells.
37353115	1	118	theme	causative	225:233	arg1	agent					235:239	a major causative agent	217:239	a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide	217:350	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	1	118	theme	causative	225:233	arg1	virus					202:206	Hepatitis C virus	190:206	Hepatitis C virus (HCV)	190:212	Hepatitis C virus (HCV) is a major causative agent of chronic liver diseases including chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma worldwide.
37353115	9	119	from	stability	1476:1484	arg1	range					1491:1495	a range	1489:1495	a range of 40.7 mV	1489:1506	The synthesized chitosan nanoparticles showed two dispersion peaks, at 196 ± 29 nm and 1.33 ± 0.36 μm, with a zeta potential of +3.3 ± 0.4mV with high stability in a range of 40.7 mV.
37353115	3	120	theme	similar	696:702	arg1	recurrence					684:693	the hepatitis C virus recurrence	662:693	the hepatitis C virus recurrence	662:693	However, antiviral resistance has become a critical issue in the treatment of chronic hepatitis C after receiving therapy with direct-acting antivirals (DAA) with a 0.5 % chance of the hepatitis C virus recurrence, similar to other chronic viral infections.
37353115	4	121	theme	crucial	803:809	arg1	issues					811:816	crucial issues	803:816	crucial issues	803:816	So, retreatment options following treatment failure have become crucial issues.
37389444	16	0	from	machinery	2886:2894	arg1	Alphaproteobacteria					2923:2941	this and possibly other Alphaproteobacteria	2899:2941	this and possibly other Alphaproteobacteria	2899:2941	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	11	1	theme	valuable	2053:2060	arg1	compounds					2062:2070	valuable compounds	2053:2070	valuable compounds	2053:2070	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	14	2	theme	envelope	2588:2595	arg1	synthesis					2597:2605	cell envelope synthesis	2583:2605	cell envelope synthesis	2583:2605	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	15	3	theme	envelope	2710:2717	arg1	peptidoglycan					2727:2739	the cell envelope polymer peptidoglycan	2701:2739	the cell envelope polymer peptidoglycan	2701:2739	We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule.
37389444	3	4	theme	outer	500:504	arg1	OM					516:517	OM	516:517	OM	516:517	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	3	4	theme	outer	500:504	arg1	membrane					506:513	an outer membrane	497:513	an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG)	497:575	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	1	5	theme	cell	257:260	arg1	morphology					262:271	cell morphology	257:271	cell morphology	257:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	7	6	from	sensitivity	1166:1176	arg1	formation					1223:1231	the formation	1219:1231	the formation of OM protrusions at the septum during cell division	1219:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	7	6	from	sensitivity	1166:1176	arg1	division					1156:1163	cell division	1151:1163	cell division	1151:1163	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	8	7	theme	cell	1542:1545	arg1	division					1547:1554	cell division	1542:1554	cell division	1542:1554	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	16	8	theme	DalA	2835:2838	arg1	analysis					2823:2830	The analysis	2819:2830	The analysis of DalA	2819:2838	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	14	9	theme	Rhodobacter	2395:2405	arg1	sphaeroides					2407:2417	Rhodobacter sphaeroides	2395:2417	Rhodobacter sphaeroides RSP_1200	2395:2426	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	16	10	theme	new	2849:2851	arg1	information					2853:2863	new information	2849:2863	new information on the cell division machinery in this and possibly other Alphaproteobacteria	2849:2941	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	5	11	theme	FtsZ	824:827	arg1	rings					829:833	FtsZ rings	824:833	FtsZ rings	824:833	We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides' divisome.
37389444	15	12	with	complex	2647:2653	arg1	proteins					2660:2667	proteins	2660:2667	proteins needed for cell division	2660:2692	We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule.
37389444	15	13	theme	cell	2680:2683	arg1	division					2685:2692	cell division	2680:2692	cell division	2680:2692	We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule.
37389444	12	14	from	differences	2152:2162	arg1	function					2189:2196	function	2189:2196	function	2189:2196	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	12	14	from	differences	2152:2162	arg1	composition					2173:2183	composition	2173:2183	composition	2173:2183	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	1	15	theme	important	189:197	arg1	roles					199:203	important roles	189:203	important roles in antibiotic resistance, nutrient acquisition, and cell morphology	189:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	7	16	from	results	1208:1214	arg1	formation					1223:1231	the formation	1219:1231	the formation of OM protrusions at the septum during cell division	1219:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	7	16	from	results	1208:1214	arg1	division					1156:1163	cell division	1151:1163	cell division	1151:1163	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	14	17	theme	cell	2583:2586	arg1	synthesis					2597:2605	cell envelope synthesis	2583:2605	cell envelope synthesis	2583:2605	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	10	18	theme	this	1711:1714	arg1	analysis					1699:1706	further analysis	1691:1706	further analysis of this and related proteins	1691:1735	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	8	19	theme	division-associated	1349:1367	arg1	A					1381:1381	division-associated lipoprotein A	1349:1381	division-associated lipoprotein A	1349:1381	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	9	20	theme	order	1630:1634	arg1	members					1599:1605	members	1599:1605	members of the Rhodobacterales order within Alphaproteobacteria	1599:1661	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	11	21	theme	IMPORTANCE	1875:1884	arg1	complexes					1900:1908	IMPORTANCE Multi-protein complexes	1875:1908	IMPORTANCE Multi-protein complexes of the bacterial cell envelope	1875:1939	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	1	22	from	roles	199:203	arg1	acquisition					240:250	nutrient acquisition	231:250	nutrient acquisition	231:250	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	1	22	from	roles	199:203	arg1	morphology					262:271	cell morphology	257:271	cell morphology	257:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	1	22	from	roles	199:203	arg1	resistance					219:228	antibiotic resistance	208:228	antibiotic resistance	208:228	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	9	23	located	present	1588:1594	arg1	members					1599:1605	members	1599:1605	members of the Rhodobacterales order within Alphaproteobacteria	1599:1661	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	9	23	located	present	1588:1594	arg2	homologs					1575:1582	DalA homologs	1570:1582	DalA homologs	1570:1582	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	11	24	theme	antimicrobial	2009:2021	arg1	resistance					2023:2032	antimicrobial resistance	2009:2032	antimicrobial resistance	2009:2032	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	7	25	theme	cell	1272:1275	arg1	division					1277:1284	cell division	1272:1284	cell division	1272:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	11	26	theme	cell	1927:1930	arg1	envelope					1932:1939	the bacterial cell envelope	1913:1939	the bacterial cell envelope	1913:1939	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	1	27	theme	cell	137:140	arg1	compartment					172:182	a key subcellular compartment	154:182	a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology	154:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	1	27	theme	cell	137:140	arg1	envelope					142:149	The bacterial cell envelope	123:149	The bacterial cell envelope	123:149	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	14	28	theme	cell	2517:2520	arg1	division					2522:2529	cell division	2517:2529	cell division	2517:2529	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	10	29	theme	related	1720:1726	arg1	proteins					1728:1735	related proteins	1720:1735	related proteins	1720:1735	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	12	30	from	variations	2212:2221	arg1	shape					2253:2257	shape	2253:2257	shape	2253:2257	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	12	30	from	variations	2212:2221	arg1	proliferation					2264:2276	proliferation	2264:2276	proliferation	2264:2276	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	12	30	from	variations	2212:2221	arg1	composition					2240:2250	cell envelope composition	2226:2250	cell envelope composition	2226:2250	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	1	31	theme	key	156:158	arg1	compartment					172:182	a key subcellular compartment	154:182	a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology	154:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	1	31	theme	key	156:158	arg1	envelope					142:149	The bacterial cell envelope	123:149	The bacterial cell envelope	123:149	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	6	32	theme	Additional	937:946	arg1	support					948:954	Additional support	937:954	Additional support for this hypothesis	937:974	Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases.
37389444	12	33	theme	cell	2226:2229	arg1	composition					2240:2250	cell envelope composition	2226:2250	cell envelope composition	2226:2250	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	0	34	theme	cell	89:92	arg1	division					94:101	normal cell division	82:101	normal cell division in Rhodobacterales	82:120	A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales.
37389444	7	35	from	septum	1258:1263	arg1	formation					1223:1231	the formation	1219:1231	the formation of OM protrusions at the septum during cell division	1219:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	7	35	from	septum	1258:1263	arg1	protrusions					1239:1249	OM protrusions	1236:1249	OM protrusions at the septum during cell division	1236:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	4	36	theme	cell	747:750	arg1	division					752:759	cell division	747:759	cell division	747:759	Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division.
37389444	2	37	theme	envelope	369:376	arg1	function					348:355	the function	344:355	the function of the cell envelope in Alphaproteobacteria	344:399	We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria.
37389444	16	38	theme	cell	2872:2875	arg1	machinery					2886:2894	the cell division machinery	2868:2894	the cell division machinery in this and possibly other Alphaproteobacteria	2868:2941	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	5	39	theme	identified	883:892	arg1	component					894:902	a newly identified component	875:902	a newly identified component of the R. sphaeroides' divisome	875:934	We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides' divisome.
37389444	5	39	theme	identified	883:892	arg1	RSP_1200					863:870	RSP_1200	863:870	RSP_1200	863:870	We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides' divisome.
37389444	7	40	theme	protrusions	1239:1249	arg1	formation					1223:1231	the formation	1219:1231	the formation of OM protrusions at the septum during cell division	1219:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	14	41	theme	protein	2491:2497	arg1	loss					2478:2481	loss	2478:2481	loss of this protein	2478:2497	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	3	42	theme	uncharacterized	459:473	arg1	lipoprotein					520:530	an outer membrane (OM) lipoprotein	497:530	an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG)	497:575	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	3	42	theme	uncharacterized	459:473	arg1	RSP_1200					484:491	RSP_1200	484:491	RSP_1200	484:491	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	3	42	theme	uncharacterized	459:473	arg1	protein					475:481	a previously uncharacterized protein	446:481	a previously uncharacterized protein	446:481	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	0	43	theme	uncharacterized	13:27	arg1	DalA					62:65	DalA	62:65	DalA	62:65	A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales.
37389444	0	43	theme	uncharacterized	13:27	arg1	lipoprotein					49:59	A previously uncharacterized divisome-associated lipoprotein	0:59	A previously uncharacterized divisome-associated lipoprotein	0:59	A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales.
37389444	3	44	theme	Rhodobacter	408:418	arg1	sphaeroides					420:430	Rhodobacter sphaeroides	408:430	Rhodobacter sphaeroides	408:430	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	16	45	theme	this	2899:2902	arg1	Alphaproteobacteria					2923:2941	this and possibly other Alphaproteobacteria	2899:2941	this and possibly other Alphaproteobacteria	2899:2941	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	6	46	with	co-precipitation	989:1004	arg1	FtsZ					1023:1026	FtsZ	1023:1026	FtsZ	1023:1026	Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases.
37389444	6	46	with	co-precipitation	989:1004	arg1	protein					1037:1043	the Pal protein	1029:1043	the Pal protein	1029:1043	Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases.
37389444	6	46	with	co-precipitation	989:1004	arg1	D-transpeptidases					1073:1089	D-transpeptidases	1073:1089	D-transpeptidases	1073:1089	Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases.
37389444	7	47	from	formation	1223:1231	arg1	septum					1258:1263	the septum	1254:1263	the septum during cell division	1254:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	8	48	theme	transpeptidases	1472:1486	arg1	activity					1457:1464	the activity	1453:1464	the activity of PG transpeptidases that are needed to form envelope invaginations during cell division	1453:1554	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	12	49	theme	protein	2095:2101	arg1	complexes					2103:2111	these protein complexes	2089:2111	these protein complexes	2089:2111	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	1	50	with	compartment	172:182	arg1	roles					199:203	important roles	189:203	important roles in antibiotic resistance, nutrient acquisition, and cell morphology	189:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	10	51	theme	macromolecular	1776:1789	arg1	machinery					1791:1799	the macromolecular machinery	1772:1799	the macromolecular machinery	1772:1799	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	3	52	theme	membrane	506:513	arg1	lipoprotein					520:530	an outer membrane (OM) lipoprotein	497:530	an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG)	497:575	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	3	52	theme	membrane	506:513	arg1	protein					475:481	a previously uncharacterized protein	446:481	a previously uncharacterized protein	446:481	Using Rhodobacter sphaeroides, we show that a previously uncharacterized protein, RSP_1200, is an outer membrane (OM) lipoprotein that non-covalently binds peptidoglycan (PG).
37389444	13	53	contain	have	2327:2330	arg1	subunits					2318:2325	some envelope protein complex subunits	2288:2325	some envelope protein complex subunits	2288:2325	However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny.
37389444	13	53	contain	have	2327:2330	arg2	homologs					2341:2348	no known homologs	2332:2348	no known homologs	2332:2348	However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny.
37389444	13	54	theme	protein	2302:2308	arg1	subunits					2318:2325	some envelope protein complex subunits	2288:2325	some envelope protein complex subunits	2288:2325	However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny.
37389444	7	55	theme	PG-active	1181:1189	arg1	antibiotics					1191:1201	PG-active antibiotics	1181:1201	PG-active antibiotics	1181:1201	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	4	56	theme	tagged	600:605	arg1	version					607:613	a fluorescently tagged version	584:613	a fluorescently tagged version of this protein	584:629	Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division.
37389444	15	57	theme	cell	2705:2708	arg1	peptidoglycan					2727:2739	the cell envelope polymer peptidoglycan	2701:2739	the cell envelope polymer peptidoglycan	2701:2739	We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule.
37389444	11	58	theme	compounds	2062:2070	arg1	formation					1998:2006	biofilm formation	1990:2006	biofilm formation	1990:2006	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	11	58	theme	compounds	2062:2070	arg1	growth					1972:1977	growth	1972:1977	growth	1972:1977	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	11	58	theme	compounds	2062:2070	arg1	production					2039:2048	production	2039:2048	production of valuable compounds	2039:2070	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	11	58	theme	compounds	2062:2070	arg1	resistance					2023:2032	antimicrobial resistance	2009:2032	antimicrobial resistance	2009:2032	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	11	58	theme	compounds	2062:2070	arg1	division					1980:1987	division	1980:1987	division	1980:1987	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	6	59	theme	Pal	1033:1035	arg1	protein					1037:1043	the Pal protein	1029:1043	the Pal protein	1029:1043	Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases.
37389444	8	60	theme	envelope	1512:1519	arg1	invaginations					1521:1533	envelope invaginations	1512:1533	envelope invaginations	1512:1533	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	11	61	theme	key	1953:1955	arg1	processes					1957:1965	key processes	1953:1965	key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds	1953:2070	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	14	62	theme	sphaeroides	2407:2417	arg1	lipoprotein					2450:2460	a newly identified lipoprotein	2431:2460	a newly identified lipoprotein (DalA)	2431:2467	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	14	62	theme	sphaeroides	2407:2417	arg1	RSP_1200					2419:2426	Rhodobacter sphaeroides RSP_1200	2395:2426	Rhodobacter sphaeroides RSP_1200	2395:2426	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	10	63	theme	Gram-negative	1851:1863	arg1	bacteria					1865:1872	Gram-negative bacteria	1851:1872	Gram-negative bacteria	1851:1872	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	11	64	theme	envelope	1932:1939	arg1	complexes					1900:1908	IMPORTANCE Multi-protein complexes	1875:1908	IMPORTANCE Multi-protein complexes of the bacterial cell envelope	1875:1939	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	1	65	theme	subcellular	160:170	arg1	compartment					172:182	a key subcellular compartment	154:182	a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology	154:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	1	65	theme	subcellular	160:170	arg1	envelope					142:149	The bacterial cell envelope	123:149	The bacterial cell envelope	123:149	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	9	66	theme	DalA	1570:1573	arg1	homologs					1575:1582	DalA homologs	1570:1582	DalA homologs	1570:1582	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	2	67	from	function	348:355	arg1	Alphaproteobacteria					381:399	Alphaproteobacteria	381:399	Alphaproteobacteria	381:399	We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria.
37389444	15	68	theme	macromolecule	2804:2816	arg1	assembly					2787:2794	the assembly	2783:2794	the assembly of this macromolecule	2783:2816	We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule.
37389444	10	69	theme	further	1691:1697	arg1	analysis					1699:1706	further analysis	1691:1706	further analysis of this and related proteins	1691:1735	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	5	70	theme	rings	829:833	arg1	location					812:819	the location	808:819	the location of FtsZ rings	808:833	We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides' divisome.
37389444	2	71	theme	better	292:297	arg1	understanding					299:311	a better understanding	290:311	a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria	290:399	We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria.
37389444	5	72	theme	RSP_1200	791:798	arg1	position					779:786	the position	775:786	the position of RSP_1200	775:798	We show that the position of RSP_1200 mirrors the location of FtsZ rings, leading us to propose that RSP_1200 is a newly identified component of the R. sphaeroides' divisome.
37389444	12	73	attach	linked	2202:2207	arg2	differences					2152:2162	differences	2152:2162	differences in their composition and function	2152:2196	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	12	73	attach	linked	2202:2207	arg1	variations					2212:2221	variations	2212:2221	variations in cell envelope composition, shape, and proliferation	2212:2276	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	11	74	theme	Multi-protein	1886:1898	arg1	complexes					1900:1908	IMPORTANCE Multi-protein complexes	1875:1908	IMPORTANCE Multi-protein complexes of the bacterial cell envelope	1875:1939	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	1	75	theme	antibiotic	208:217	arg1	resistance					219:228	antibiotic resistance	208:228	antibiotic resistance	208:228	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	11	76	theme	biofilm	1990:1996	arg1	formation					1998:2006	biofilm formation	1990:2006	biofilm formation	1990:2006	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	8	77	theme	lipoprotein	1369:1379	arg1	A					1381:1381	division-associated lipoprotein A	1349:1381	division-associated lipoprotein A	1349:1381	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	4	78	theme	protein	623:629	arg1	version					607:613	a fluorescently tagged version	584:613	a fluorescently tagged version of this protein	584:629	Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division.
37389444	16	79	from	information	2853:2863	arg1	machinery					2886:2894	the cell division machinery	2868:2894	the cell division machinery in this and possibly other Alphaproteobacteria	2868:2941	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	9	80	theme	Rhodobacterales	1614:1628	arg1	order					1630:1634	the Rhodobacterales order	1610:1634	the Rhodobacterales order within Alphaproteobacteria	1610:1661	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	7	81	from	defects	1140:1146	arg1	formation					1223:1231	the formation	1219:1231	the formation of OM protrusions at the septum during cell division	1219:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	7	81	from	defects	1140:1146	arg1	division					1156:1163	cell division	1151:1163	cell division	1151:1163	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	15	82	theme	polymer	2719:2725	arg1	peptidoglycan					2727:2739	the cell envelope polymer peptidoglycan	2701:2739	the cell envelope polymer peptidoglycan	2701:2739	We find that DalA forms a complex with proteins needed for cell division, binds the cell envelope polymer peptidoglycan, and colocalizes with enzymes involved in the assembly of this macromolecule.
37389444	8	83	theme	name	1325:1328	arg1	DalA					1339:1342	name RSP_1200 DalA	1325:1342	name RSP_1200 DalA (for division-associated lipoprotein A)	1325:1382	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	11	84	theme	bacterial	1917:1925	arg1	envelope					1932:1939	the bacterial cell envelope	1913:1939	the bacterial cell envelope	1913:1939	IMPORTANCE Multi-protein complexes of the bacterial cell envelope orchestrate key processes like growth, division, biofilm formation, antimicrobial resistance, and production of valuable compounds.
37389444	1	85	theme	bacterial	127:135	arg1	compartment					172:182	a key subcellular compartment	154:182	a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology	154:271	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	1	85	theme	bacterial	127:135	arg1	envelope					142:149	The bacterial cell envelope	123:149	The bacterial cell envelope	123:149	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	0	86	from	division	94:101	arg1	Rhodobacterales					106:120	Rhodobacterales	106:120	Rhodobacterales	106:120	A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales.
37389444	4	87	theme	cell	699:702	arg1	cycle					704:708	the cell cycle	695:708	the cell cycle	695:708	Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division.
37389444	7	88	theme	cell	1151:1154	arg1	division					1156:1163	cell division	1151:1163	cell division	1151:1163	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	10	89	theme	proteins	1728:1735	arg1	analysis					1699:1706	further analysis	1691:1706	further analysis of this and related proteins	1691:1735	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	0	90	theme	normal	82:87	arg1	division					94:101	normal cell division	82:101	normal cell division in Rhodobacterales	82:120	A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales.
37389444	9	91	from	members	1599:1605	arg1	present					1588:1594	present	1588:1594	present	1588:1594	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	12	92	theme	envelope	2231:2238	arg1	composition					2240:2250	cell envelope composition	2226:2250	cell envelope composition	2226:2250	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
37389444	4	93	theme	dynamic	666:672	arg1	repositioning					674:686	a dynamic repositioning	664:686	a dynamic repositioning	664:686	Using a fluorescently tagged version of this protein, we find that RSP_1200 undergoes a dynamic repositioning during the cell cycle and is enriched at the septum during cell division.
37389444	13	94	theme	bacterial	2361:2369	arg1	phylogeny					2371:2379	the bacterial phylogeny	2357:2379	the bacterial phylogeny	2357:2379	However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny.
37389444	9	95	from	present	1588:1594	arg1	members					1599:1605	members	1599:1605	members of the Rhodobacterales order within Alphaproteobacteria	1599:1661	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	16	96	theme	division	2877:2884	arg1	machinery					2886:2894	the cell division machinery	2868:2894	the cell division machinery in this and possibly other Alphaproteobacteria	2868:2941	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	13	97	theme	known	2335:2339	arg1	homologs					2341:2348	no known homologs	2332:2348	no known homologs	2332:2348	However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny.
37389444	7	98	theme	OM	1236:1237	arg1	protrusions					1239:1249	OM protrusions	1236:1249	OM protrusions at the septum during cell division	1236:1284	We also find that a ∆RSP_1200 mutation leads to defects in cell division, sensitivity to PG-active antibiotics, and results in the formation of OM protrusions at the septum during cell division.
37389444	2	99	theme	cell	364:367	arg1	envelope					369:376	the cell envelope	360:376	the cell envelope	360:376	We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria.
37389444	0	100	theme	divisome-associated	29:47	arg1	DalA					62:65	DalA	62:65	DalA	62:65	A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales.
37389444	0	100	theme	divisome-associated	29:47	arg1	lipoprotein					49:59	A previously uncharacterized divisome-associated lipoprotein	0:59	A previously uncharacterized divisome-associated lipoprotein	0:59	A previously uncharacterized divisome-associated lipoprotein, DalA, is needed for normal cell division in Rhodobacterales.
37389444	6	101	theme	RSP_1200	1009:1016	arg1	co-precipitation					989:1004	the co-precipitation	985:1004	the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases	985:1089	Additional support for this hypothesis includes the co-precipitation of RSP_1200 with FtsZ, the Pal protein, and several predicted PG L,D-transpeptidases.
37389444	13	102	theme	complex	2310:2316	arg1	subunits					2318:2325	some envelope protein complex subunits	2288:2325	some envelope protein complex subunits	2288:2325	However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny.
37389444	8	103	theme	PG	1469:1470	arg1	transpeptidases					1472:1486	PG transpeptidases	1469:1486	PG transpeptidases that are needed to form envelope invaginations during cell division	1469:1554	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	16	104	theme	other	2917:2921	arg1	Alphaproteobacteria					2923:2941	this and possibly other Alphaproteobacteria	2899:2941	this and possibly other Alphaproteobacteria	2899:2941	The analysis of DalA provides new information on the cell division machinery in this and possibly other Alphaproteobacteria.
37389444	9	105	attach	present	1588:1594	arg1	members					1599:1605	members	1599:1605	members of the Rhodobacterales order within Alphaproteobacteria	1599:1661	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	9	105	attach	present	1588:1594	arg2	homologs					1575:1582	DalA homologs	1570:1582	DalA homologs	1570:1582	We find that DalA homologs are present in members of the Rhodobacterales order within Alphaproteobacteria.
37389444	8	106	theme	RSP_1200	1330:1337	arg1	DalA					1339:1342	name RSP_1200 DalA	1325:1342	name RSP_1200 DalA (for division-associated lipoprotein A)	1325:1382	Based on these results, we propose to name RSP_1200 DalA (for division-associated lipoprotein A) and postulate that DalA serves as a scaffold to position or modulate the activity of PG transpeptidases that are needed to form envelope invaginations during cell division.
37389444	10	107	theme	machinery	1791:1799	arg1	understanding					1755:1767	our understanding	1751:1767	our understanding of the macromolecular machinery	1751:1799	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	10	107	theme	machinery	1791:1799	arg1	proteins					1805:1812	proteins	1805:1812	proteins that participate in cell division in Gram-negative bacteria	1805:1872	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	2	108	theme	proteins	316:323	arg1	understanding					299:311	a better understanding	290:311	a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria	290:399	We seek to gain a better understanding of proteins that contribute to the function of the cell envelope in Alphaproteobacteria.
37389444	1	109	theme	nutrient	231:238	arg1	acquisition					240:250	nutrient acquisition	231:250	nutrient acquisition	231:250	The bacterial cell envelope is a key subcellular compartment with important roles in antibiotic resistance, nutrient acquisition, and cell morphology.
37389444	14	110	theme	identified	2439:2448	arg1	DalA					2463:2466	DalA	2463:2466	DalA	2463:2466	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	14	110	theme	identified	2439:2448	arg1	lipoprotein					2450:2460	a newly identified lipoprotein	2431:2460	a newly identified lipoprotein (DalA)	2431:2467	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	14	110	theme	identified	2439:2448	arg1	RSP_1200					2419:2426	Rhodobacter sphaeroides RSP_1200	2395:2426	Rhodobacter sphaeroides RSP_1200	2395:2426	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	13	111	theme	envelope	2293:2300	arg1	subunits					2318:2325	some envelope protein complex subunits	2288:2325	some envelope protein complex subunits	2288:2325	However, some envelope protein complex subunits have no known homologs across the bacterial phylogeny.
37389444	14	112	from	defects	2506:2512	arg1	division					2522:2529	cell division	2517:2529	cell division	2517:2529	We find that Rhodobacter sphaeroides RSP_1200 is a newly identified lipoprotein (DalA) and that loss of this protein causes defects in cell division and changes the sensitivity to compounds, affecting cell envelope synthesis and function.
37389444	10	113	theme	cell	1834:1837	arg1	division					1839:1846	cell division	1834:1846	cell division	1834:1846	Therefore, we propose that further analysis of this and related proteins will increase our understanding of the macromolecular machinery and proteins that participate in cell division in Gram-negative bacteria.
37389444	12	114	theme	complexes	2103:2111	arg1	subunits					2077:2084	The subunits	2073:2084	The subunits of these protein complexes	2073:2111	The subunits of these protein complexes are well studied in some bacteria, and differences in their composition and function are linked to variations in cell envelope composition, shape, and proliferation.
35956737	0	0	theme	Synthetic	78:86	arg1	Strategy					124:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	7	1	theme	composite	1167:1175	arg1	effect					1177:1182	a composite effect	1165:1182	a composite effect	1165:1182	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	5	2	theme	Aureobasidium	923:935	arg1	cocultivation					877:889	cocultivation	877:889	cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers	877:967	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	7	3	theme	significant	1244:1254	arg1	improvement					1256:1266	a significant improvement	1242:1266	a significant improvement in mechanical properties	1242:1291	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	6	4	theme	infrared	1115:1122	arg1	spectroscopy					1124:1135	Fourier transformed infrared spectroscopy	1095:1135	Fourier transformed infrared spectroscopy	1095:1135	The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy.
35956737	6	5	theme	PUL	1020:1022	arg1	inclusion					1007:1015	The inclusion	1003:1015	The inclusion of PUL in BC	1003:1028	The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy.
35956737	0	6	theme	Microbial	104:112	arg1	Strategy					124:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	2	7	theme	nanofibrillar	296:308	arg1	structure					310:318	The micro- and nanofibrillar structure	281:318	The micro- and nanofibrillar structure	281:318	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	8	8	theme	better	1421:1426	arg1	quality					1428:1434	better quality	1421:1434	better quality	1421:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	0	9	theme	Biology-Powered	88:102	arg1	Strategy					124:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	8	10	theme	grafting	1322:1329	arg1	"					1330:1330	"grafting"	1321:1330	"grafting" of prebiotic activity	1321:1352	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	3	11	theme	prebiotic	549:557	arg1	system					532:537	a cocultivation system	516:537	a cocultivation system of BC and prebiotic producers	516:567	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
35956737	5	12	theme	PUL	955:957	arg1	cocultivation					877:889	cocultivation	877:889	cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers	877:967	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	8	13	theme	developed	1298:1306	arg1	approach					1308:1315	The developed approach	1294:1315	The developed approach for "grafting" of prebiotic activity on BC	1294:1358	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	2	14	theme	micro-	285:290	arg1	structure					310:318	The micro- and nanofibrillar structure	281:318	The micro- and nanofibrillar structure	281:318	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	6	15	theme	transformed	1103:1113	arg1	spectroscopy					1124:1135	Fourier transformed infrared spectroscopy	1095:1135	Fourier transformed infrared spectroscopy	1095:1135	The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy.
35956737	0	16	theme	Coculture	114:122	arg1	Strategy					124:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Synthetic Biology-Powered Microbial Coculture Strategy	78:131	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	6	17	from	inclusion	1007:1015	arg1	BC					1027:1028	BC	1027:1028	BC	1027:1028	The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy.
35956737	3	18	dep	BC	542:543	arg1	producers					559:567	producers	559:567	producers	559:567	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
35956737	3	19	theme	BC	542:543	arg1	system					532:537	a cocultivation system	516:537	a cocultivation system of BC and prebiotic producers	516:567	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
35956737	4	20	dep	polysaccharides	779:793	arg1	polysaccharides					779:793	the other microbial polysaccharides gellan and xanthan	759:812	the other microbial polysaccharides gellan and xanthan	759:812	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	20	dep	polysaccharides	779:793	arg1	xanthan					806:812	xanthan	806:812	xanthan	806:812	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	20	dep	polysaccharides	779:793	arg1	gellan					795:800	gellan	795:800	gellan	795:800	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	7	21	theme	BC	1218:1219	arg1	fibers					1221:1226	BC fibers	1218:1226	BC fibers	1218:1226	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	7	22	dep	aggregation	1191:1201	arg1	the					1187:1189	the	1187:1189	the	1187:1189	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	5	23	with	biocomposite	822:833	arg1	properties					850:859	prebiotic properties	840:859	prebiotic properties	840:859	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	0	24	theme	Advanced	0:7	arg1	Composite					27:35	Advanced "Green" Prebiotic Composite	0:35	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan	0:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	0	24	theme	Advanced	0:7	arg1	Cellulose/Pullulan					50:67	Bacterial Cellulose/Pullulan	40:67	Bacterial Cellulose/Pullulan	40:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	5	25	dep	Aureobasidium	923:935	arg1	BC					948:949	BC	948:949	BC	948:949	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	5	25	dep	Aureobasidium	923:935	arg1	pullulans					937:945	Aureobasidium pullulans, BC	923:949	pullulans	937:945	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	2	26	theme	BC	323:324	arg1	structure					310:318	The micro- and nanofibrillar structure	281:318	The micro- and nanofibrillar structure	281:318	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	8	27	theme	friendly	1398:1405	arg1	composites					1407:1416	environmentally friendly composites	1382:1416	environmentally friendly composites of better quality	1382:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	8	27	theme	friendly	1398:1405	arg1	quality					1428:1434	better quality	1421:1434	better quality	1421:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	1	28	dep	Komagataeibacter	247:262	arg1	xylinus					264:270	Komagataeibacter xylinus	247:270	Komagataeibacter xylinus	247:270	Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used.
35956737	0	29	theme	"	15:15	arg1	Composite					27:35	Advanced "Green" Prebiotic Composite	0:35	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan	0:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	0	29	theme	"	15:15	arg1	Cellulose/Pullulan					50:67	Bacterial Cellulose/Pullulan	40:67	Bacterial Cellulose/Pullulan	40:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	5	30	theme	BC/PUL	815:820	arg1	biocomposite					822:833	BC/PUL biocomposite	815:833	BC/PUL biocomposite with prebiotic properties	815:859	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	6	31	theme	scanning	1059:1066	arg1	microscopy					1077:1086	scanning electron microscopy	1059:1086	scanning electron microscopy	1059:1086	The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy.
35956737	5	32	theme	prebiotic	840:848	arg1	properties					850:859	prebiotic properties	840:859	prebiotic properties	840:859	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	0	33	theme	Green	10:14	arg1	Composite					27:35	Advanced "Green" Prebiotic Composite	0:35	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan	0:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	0	33	theme	Green	10:14	arg1	Cellulose/Pullulan					50:67	Bacterial Cellulose/Pullulan	40:67	Bacterial Cellulose/Pullulan	40:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	4	34	theme	microbial	769:777	arg1	polysaccharides					779:793	the other microbial polysaccharides gellan and xanthan	759:812	the other microbial polysaccharides gellan and xanthan	759:812	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	34	theme	microbial	769:777	arg1	xanthan					806:812	xanthan	806:812	xanthan	806:812	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	34	theme	microbial	769:777	arg1	gellan					795:800	gellan	795:800	gellan	795:800	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	7	35	theme	mechanical	1271:1280	arg1	properties					1282:1291	mechanical properties	1271:1291	mechanical properties	1271:1291	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	2	36	theme	other	468:472	arg1	microorganisms					474:487	other microorganisms	468:487	other microorganisms	468:487	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	6	37	theme	electron	1068:1075	arg1	microscopy					1077:1086	scanning electron microscopy	1059:1086	scanning electron microscopy	1059:1086	The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy.
35956737	8	38	theme	environmentally	1382:1396	arg1	composites					1407:1416	environmentally friendly composites	1382:1416	environmentally friendly composites of better quality	1382:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	8	38	theme	environmentally	1382:1396	arg1	quality					1428:1434	better quality	1421:1434	better quality	1421:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	2	39	theme	many	339:342	arg1	pores					360:364	many different-sized pores	339:364	many different-sized pores	339:364	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	1	40	used	used	275:278	arg2	Komagataeibacter					247:262	Komagataeibacter	247:262	Komagataeibacter	247:262	Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used.
35956737	1	41	theme	different	187:195	arg1	microorganisms					197:210	different microorganisms	187:210	different microorganisms	187:210	Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used.
35956737	2	42	theme	polymers	419:426	arg1	introduction					397:408	the introduction	393:408	the introduction of other polymers into it	393:434	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	0	43	theme	Prebiotic	17:25	arg1	Composite					27:35	Advanced "Green" Prebiotic Composite	0:35	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan	0:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	0	43	theme	Prebiotic	17:25	arg1	Cellulose/Pullulan					50:67	Bacterial Cellulose/Pullulan	40:67	Bacterial Cellulose/Pullulan	40:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	8	44	theme	activity	1345:1352	arg1	"					1330:1330	"grafting"	1321:1330	"grafting" of prebiotic activity	1321:1352	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	3	45	theme	prebiotic	612:620	arg1	activity					622:629	prebiotic activity	612:629	prebiotic activity	612:629	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
35956737	2	46	theme	other	413:417	arg1	polymers					419:426	other polymers	413:426	other polymers	413:426	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	0	47	theme	Bacterial	40:48	arg1	Cellulose/Pullulan					50:67	Bacterial Cellulose/Pullulan	40:67	Bacterial Cellulose/Pullulan	40:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	4	48	located	found	666:670	arg1	study					640:644	this study	635:644	this study	635:644	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	48	located	found	666:670	arg2	PUL					657:659	PUL	657:659	PUL	657:659	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	48	located	found	666:670	arg2	pullulan					647:654	pullulan	647:654	pullulan (PUL)	647:660	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	3	49	theme	composite	588:596	arg1	material					598:605	BC-based composite material	579:605	BC-based composite material with prebiotic activity	579:629	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
35956737	8	50	theme	composites	1407:1416	arg1	preparation					1367:1377	preparation	1367:1377	preparation of environmentally friendly composites of better quality	1367:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	2	51	theme	different-sized	344:358	arg1	pores					360:364	many different-sized pores	339:364	many different-sized pores	339:364	The micro- and nanofibrillar structure of BC, which forms many different-sized pores, creates prerequisites for the introduction of other polymers into it, including those synthesized by other microorganisms.
35956737	1	52	theme	Bacterial	134:142	arg1	cellulose					144:152	Bacterial cellulose	134:152	Bacterial cellulose (BC)	134:157	Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used.
35956737	1	52	theme	Bacterial	134:142	arg1	biopolymer					164:173	a biopolymer	162:173	a biopolymer produced by different microorganisms	162:210	Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used.
35956737	1	52	theme	Bacterial	134:142	arg1	BC					155:156	BC	155:156	BC	155:156	Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used.
35956737	7	53	from	effect	1177:1182	arg1	aggregation					1191:1201	aggregation	1191:1201	aggregation	1191:1201	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	7	53	from	effect	1177:1182	arg1	binding					1207:1213	binding	1207:1213	binding	1207:1213	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	5	54	dep	Komagataeibacter	894:909	arg1	producers					959:967	producers	959:967	producers	959:967	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	5	54	dep	Komagataeibacter	894:909	arg1	xylinus					911:917	Komagataeibacter xylinus	894:917	Komagataeibacter xylinus	894:917	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	4	55	theme	strain	713:718	arg1	growth					689:694	the growth	685:694	the growth of the probiotic strain	685:718	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	5	56	theme	Komagataeibacter	894:909	arg1	cocultivation					877:889	cocultivation	877:889	cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers	877:967	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	1	57	theme	biotechnological	220:235	arg1	practice					237:244	biotechnological practice	220:244	biotechnological practice	220:244	Bacterial cellulose (BC) is a biopolymer produced by different microorganisms, but in biotechnological practice, Komagataeibacter xylinus is used.
35956737	4	58	theme	other	763:767	arg1	polysaccharides					779:793	the other microbial polysaccharides gellan and xanthan	759:812	the other microbial polysaccharides gellan and xanthan	759:812	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	58	theme	other	763:767	arg1	xanthan					806:812	xanthan	806:812	xanthan	806:812	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	4	58	theme	other	763:767	arg1	gellan					795:800	gellan	795:800	gellan	795:800	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	7	59	from	improvement	1256:1266	arg1	properties					1282:1291	mechanical properties	1271:1291	mechanical properties	1271:1291	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	5	60	theme	molasses	986:993	arg1	medium					995:1000	molasses medium	986:1000	molasses medium	986:1000	BC/PUL biocomposite with prebiotic properties was obtained by cocultivation of Komagataeibacter xylinus and Aureobasidium pullulans, BC and PUL producers respectively, on molasses medium.
35956737	4	61	theme	probiotic	703:711	arg1	strain					713:718	the probiotic strain	699:718	the probiotic strain	699:718	In this study, pullulan (PUL) was found to stimulate the growth of the probiotic strain Lactobacillus rhamnosus GG better than the other microbial polysaccharides gellan and xanthan.
35956737	8	62	theme	prebiotic	1335:1343	arg1	activity					1345:1352	prebiotic activity	1335:1352	prebiotic activity	1335:1352	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	8	63	theme	quality	1428:1434	arg1	composites					1407:1416	environmentally friendly composites	1382:1416	environmentally friendly composites of better quality	1382:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	8	63	theme	quality	1428:1434	arg1	quality					1428:1434	better quality	1421:1434	better quality	1421:1434	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	3	64	theme	cocultivation	518:530	arg1	system					532:537	a cocultivation system	516:537	a cocultivation system of BC and prebiotic producers	516:567	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
35956737	6	65	theme	Fourier	1095:1101	arg1	spectroscopy					1124:1135	Fourier transformed infrared spectroscopy	1095:1135	Fourier transformed infrared spectroscopy	1095:1135	The inclusion of PUL in BC is proved gravimetrically by scanning electron microscopy and by Fourier transformed infrared spectroscopy.
35956737	0	66	theme	Cellulose/Pullulan	50:67	arg1	Composite					27:35	Advanced "Green" Prebiotic Composite	0:35	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan	0:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	0	66	theme	Cellulose/Pullulan	50:67	arg1	Cellulose/Pullulan					50:67	Bacterial Cellulose/Pullulan	40:67	Bacterial Cellulose/Pullulan	40:67	Advanced "Green" Prebiotic Composite of Bacterial Cellulose/Pullulan Based on Synthetic Biology-Powered Microbial Coculture Strategy.
35956737	8	67	from	approach	1308:1315	arg1	BC					1357:1358	BC	1357:1358	BC	1357:1358	The developed approach for "grafting" of prebiotic activity on BC allows preparation of environmentally friendly composites of better quality.
35956737	3	68	theme	BC-based	579:586	arg1	material					598:605	BC-based composite material	579:605	BC-based composite material with prebiotic activity	579:629	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
35956737	7	69	theme	fibers	1221:1226	arg1	aggregation					1191:1201	aggregation	1191:1201	aggregation	1191:1201	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	7	69	theme	fibers	1221:1226	arg1	binding					1207:1213	binding	1207:1213	binding	1207:1213	Cocultivation demonstrated a composite effect on the aggregation and binding of BC fibers, which led to a significant improvement in mechanical properties.
35956737	3	70	with	material	598:605	arg1	activity					622:629	prebiotic activity	612:629	prebiotic activity	612:629	The study aims to develop a cocultivation system of BC and prebiotic producers to obtain BC-based composite material with prebiotic activity.
36508948	6	0	theme	LMB	1022:1024	arg1	liver					1013:1017	the liver	1009:1017	the liver of LMB fed low protein high starch diets	1009:1058	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	3	1	theme	initial	617:623	arg1	weight					630:635	initial body weight	617:635	initial body weight	617:635	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	3	1	theme	initial	617:623	arg1	4.65 ± 0.01 g					638:650	4.65 ± 0.01 g	638:650	4.65 ± 0.01 g	638:650	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	13	2	theme	factors	2517:2523	arg1	release					2487:2493	The release	2483:2493	The release of these inflammatory factors	2483:2523	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	1	3	theme	liver	266:270	arg1	injury					272:277	liver injury	266:277	liver injury	266:277	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	15	4	theme	feeding	2924:2930	arg1	protein					2936:2942	feeding low protein	2924:2942	feeding low protein	2924:2942	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	7	5	theme	lipoprotein	1357:1367	arg1	lipase					1369:1374	lipoprotein lipase	1357:1374	lipoprotein lipase (representative of triglyceride synthesis, up-regulated)	1357:1431	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	11	6	theme	signal	2300:2305	arg1	pathway					2307:2313	PI3K/Akt signal pathway	2291:2313	PI3K/Akt signal pathway	2291:2313	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	8	7	theme	inferior	1607:1614	arg1	tolerance					1624:1632	inferior glucose tolerance	1607:1632	inferior glucose tolerance to treatment P49S9	1607:1651	Though fasting plasma glucose level was comparable, treatment P42S18 performed inferior glucose tolerance to treatment P49S9.
36508948	3	8	theme	juveniles	653:661	arg1	triplicate					587:596	triplicate replicates	587:607	triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles	587:661	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	15	9	theme	PI3K/Akt/NF-κB	3032:3045	arg1	pathway					3057:3063	the PI3K/Akt/NF-κB signaling pathway	3028:3063	the PI3K/Akt/NF-κB signaling pathway	3028:3063	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	2	10	dep	P42S18	523:528	arg1	starch					557:562	starch	557:562	starch	557:562	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	2	10	dep	P42S18	523:528	arg1	protein					538:544	42.4 % protein and 18.2 % starch	531:562	protein	538:544	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	7	11	theme	triglyceride	1395:1406	arg1	synthesis					1408:1416	triglyceride synthesis	1395:1416	triglyceride synthesis	1395:1416	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	13	12	dep	stimulation	2554:2564	arg1	effects					2603:2609	chemotactic effects	2591:2609	chemotactic effects	2591:2609	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	10	13	theme	Toll-like	2071:2079	arg1	receptor					2081:2088	Toll-like receptor	2071:2088	Toll-like receptor	2071:2088	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	10	14	theme	KEGG	1898:1901	arg1	analysis					1912:1919	KEGG pathways analysis	1898:1919	KEGG pathways analysis	1898:1919	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	13	15	theme	killer	2578:2583	arg1	cells					2585:2589	natural killer cells	2570:2589	natural killer cells	2570:2589	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	7	16	theme	representative	1377:1390	arg1	lipase					1369:1374	lipoprotein lipase	1357:1374	lipoprotein lipase (representative of triglyceride synthesis, up-regulated)	1357:1431	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	7	17	theme	glycerolipid	1320:1331	arg1	metabolism					1333:1342	hepatic glycerolipid metabolism	1312:1342	hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated)	1312:1525	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	0	18	theme	low	160:162	arg1	diets					184:188	low protein high starch diets	160:188	low protein high starch diets	160:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	1	19	theme	present	195:201	arg1	study					203:207	The present study	191:207	The present study	191:207	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	15	20	theme	starch	2953:2958	arg1	diets					2960:2964	high starch diets	2948:2964	high starch diets	2948:2964	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	10	21	theme	signaling	2104:2112	arg1	pathways					2114:2121	apoptosis signaling pathways	2094:2121	apoptosis signaling pathways	2094:2121	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	0	22	theme	bass	129:132	arg1	salmoides					146:154	juvenile largemouth bass Micropterus salmoides	109:154	juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	109:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	15	23	theme	signaling	3047:3055	arg1	pathway					3057:3063	the PI3K/Akt/NF-κB signaling pathway	3028:3063	the PI3K/Akt/NF-κB signaling pathway	3028:3063	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	0	24	from	inflammation	79:90	arg1	salmoides					146:154	juvenile largemouth bass Micropterus salmoides	109:154	juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	109:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	6	25	theme	enzyme	1169:1174	arg1	expression					1115:1124	down-regulated expression	1100:1124	down-regulated expression of the glycogenolytic glycogen debranching enzyme	1100:1174	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	10	26	theme	expressed	1976:1984	arg1	genes					1986:1990	the up-regulated differentially expressed genes	1944:1990	the up-regulated differentially expressed genes	1944:1990	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	5	27	theme	feeding	778:784	arg1	ratio					786:790	the feeding ratio to meet their protein requirements	774:825	the feeding ratio to meet their protein requirements	774:825	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	9	28	theme	dUTP	1694:1697	arg1	TUNEL					1718:1722	TUNEL	1718:1722	TUNEL	1718:1722	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	9	28	theme	dUTP	1694:1697	arg1	Labeling					1708:1715	TdT-mediated dUTP Nick-End Labeling	1681:1715	TdT-mediated dUTP Nick-End Labeling (TUNEL) staining	1681:1732	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	6	29	theme	glycogen	1148:1155	arg1	enzyme					1169:1174	the glycogenolytic glycogen debranching enzyme	1129:1174	the glycogenolytic glycogen debranching enzyme	1129:1174	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	6	30	theme	low	1030:1032	arg1	diets					1054:1058	low protein high starch diets	1030:1058	low protein high starch diets	1030:1058	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	10	31	theme	up-regulated	1948:1959	arg1	genes					1986:1990	the up-regulated differentially expressed genes	1944:1990	the up-regulated differentially expressed genes	1944:1990	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	1	32	from	mechanism	253:261	arg1	LMB					321:323	LMB	321:323	LMB	321:323	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	1	32	from	mechanism	253:261	arg1	salmoides					310:318	largemouth bass Micropterus salmoides	282:318	largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets	282:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	9	33	theme	Labeling	1708:1715	arg1	staining					1725:1732	TdT-mediated dUTP Nick-End Labeling (TUNEL) staining	1681:1732	TdT-mediated dUTP Nick-End Labeling (TUNEL) staining	1681:1732	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	14	34	theme	TNF-R1	2800:2805	arg1	pathway					2808:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	0	35	theme	high	172:175	arg1	diets					184:188	low protein high starch diets	160:188	low protein high starch diets	160:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	14	36	theme	c	2876:2876	arg1	expression					2837:2846	the expression	2833:2846	the expression of caspase 8 and cytochrome c	2833:2876	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	5	37	theme	growth	857:862	arg1	performance					864:874	growth performance	857:874	growth performance	857:874	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	2	38	theme	%	490:490	arg1	protein					492:498	48.8 % protein and 9.06 % starch	485:516	protein	492:498	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	0	39	from	apoptosis	96:104	arg1	salmoides					146:154	juvenile largemouth bass Micropterus salmoides	109:154	juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	109:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	14	40	theme	TNF	2795:2797	arg1	pathway					2808:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	3	41	dep	LMB	612:614	arg1	weight					630:635	initial body weight	617:635	initial body weight	617:635	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	3	41	dep	LMB	612:614	arg1	4.65 ± 0.01 g					638:650	4.65 ± 0.01 g	638:650	4.65 ± 0.01 g	638:650	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	1	42	theme	low	330:332	arg1	diets					354:358	low protein high starch diets	330:358	low protein high starch diets	330:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	13	43	theme	chemotactic	2591:2601	arg1	effects					2603:2609	chemotactic effects	2591:2609	chemotactic effects	2591:2609	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	6	44	theme	Periodic	942:949	arg1	acid-Schiff					951:961	Periodic acid-Schiff	942:961	Periodic acid-Schiff (PAS) staining	942:976	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	6	44	theme	Periodic	942:949	arg1	PAS					964:966	PAS	964:966	PAS	964:966	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	11	45	theme	protein	2173:2179	arg1	diets					2193:2197	low protein high starch diets	2169:2197	low protein high starch diets	2169:2197	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	10	46	theme	glycation	2023:2031	arg1	product					2037:2043	advanced glycation end product	2014:2043	advanced glycation end product	2014:2043	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	10	46	theme	glycation	2023:2031	arg1	AGE					2009:2011	AGE	2009:2011	AGE (advanced glycation end product)/RAGE (receptor for AGE)	2009:2068	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	0	47	theme	inflammation	79:90	arg1	mechanism					60:68	the molecular mechanism	46:68	the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	46:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	1	48	theme	high	342:345	arg1	diets					354:358	low protein high starch diets	330:358	low protein high starch diets	330:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	14	49	theme	immune	2692:2697	arg1	response					2699:2706	immune response	2692:2706	immune response	2692:2706	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	5	50	theme	feeding	828:834	arg1	ratio					847:851	feeding efficiency ratio	828:851	feeding efficiency ratio	828:851	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	0	51	theme	apoptosis	96:104	arg1	mechanism					60:68	the molecular mechanism	46:68	the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	46:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	9	52	theme	low	1757:1759	arg1	diets					1781:1785	low protein high starch diets	1757:1785	low protein high starch diets	1757:1785	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	0	53	theme	juvenile	109:116	arg1	salmoides					146:154	juvenile largemouth bass Micropterus salmoides	109:154	juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	109:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	9	54	theme	high	1769:1772	arg1	diets					1781:1785	low protein high starch diets	1757:1785	low protein high starch diets	1757:1785	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	15	55	theme	low	2932:2934	arg1	protein					2936:2942	feeding low protein	2924:2942	feeding low protein	2924:2942	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	11	56	theme	starch	2186:2191	arg1	diets					2193:2197	low protein high starch diets	2169:2197	low protein high starch diets	2169:2197	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	2	57	theme	isoenergetic	380:391	arg1	diets					393:397	Two isolipidic and isoenergetic diets	361:397	Two isolipidic and isoenergetic diets	361:397	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	0	58	theme	Transcriptome	0:12	arg1	analysis					14:21	Transcriptome analysis	0:21	Transcriptome analysis	0:21	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	1	59	theme	largemouth	282:291	arg1	LMB					321:323	LMB	321:323	LMB	321:323	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	1	59	theme	largemouth	282:291	arg1	salmoides					310:318	largemouth bass Micropterus salmoides	282:318	largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets	282:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	2	60	theme	starch	442:447	arg1	ratios					449:454	different protein and starch ratios	420:454	ratios	449:454	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	12	61	theme	proinflammatory	2398:2412	arg1	factors					2414:2420	proinflammatory factors	2398:2420	proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8	2398:2480	The activation of Akt induced NF-κB translocation into the nucleus thus releasing proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8.
36508948	12	61	theme	proinflammatory	2398:2412	arg1	necrosis					2438:2445	tumor necrosis factor-α	2432:2454	tumor necrosis factor-α (TNF-α)	2432:2462	The activation of Akt induced NF-κB translocation into the nucleus thus releasing proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8.
36508948	12	61	theme	proinflammatory	2398:2412	arg1	interleukin-8					2468:2480	interleukin-8	2468:2480	interleukin-8	2468:2480	The activation of Akt induced NF-κB translocation into the nucleus thus releasing proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8.
36508948	12	62	theme	Akt	2334:2336	arg1	activation					2320:2329	The activation	2316:2329	The activation of Akt	2316:2336	The activation of Akt induced NF-κB translocation into the nucleus thus releasing proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8.
36508948	5	63	theme	P42S18	749:754	arg1	fish					756:759	the P42S18 fish	745:759	the P42S18 fish	745:759	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	1	64	theme	Micropterus	298:308	arg1	LMB					321:323	LMB	321:323	LMB	321:323	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	1	64	theme	Micropterus	298:308	arg1	salmoides					310:318	largemouth bass Micropterus salmoides	282:318	largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets	282:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	7	65	theme	triglyceride	1487:1498	arg1	breakdown					1500:1508	triglyceride breakdown	1487:1508	triglyceride breakdown	1487:1508	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	7	66	theme	high	1241:1244	arg1	diets					1253:1257	low protein high starch diets	1229:1257	low protein high starch diets	1229:1257	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	2	67	theme	diets	472:476	arg1	P49S9					478:482	diets P49S9	472:482	diets P49S9 (48.8 % protein and 9.06 % starch)	472:517	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	13	68	theme	cell	2549:2552	arg1	stimulation					2554:2564	T cell stimulation	2547:2564	T cell stimulation	2547:2564	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	7	69	theme	representative	1469:1482	arg1	acyltransferase					1452:1466	diacylglycerol acyltransferase	1437:1466	diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated)	1437:1525	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	7	70	theme	low	1229:1231	arg1	diets					1253:1257	low protein high starch diets	1229:1257	low protein high starch diets	1229:1257	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	9	71	theme	structural	1809:1818	arg1	integrity					1820:1828	structural integrity	1809:1828	structural integrity	1809:1828	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	11	72	from	accumulation	2217:2228	arg1	LMB					2241:2243	LMB	2241:2243	LMB	2241:2243	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	0	73	theme	molecular	50:58	arg1	mechanism					60:68	the molecular mechanism	46:68	the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	46:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	2	74	dep	P49S9	478:482	arg1	starch					511:516	starch	511:516	starch	511:516	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	2	74	dep	P49S9	478:482	arg1	protein					492:498	48.8 % protein and 9.06 % starch	485:516	protein	492:498	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	6	75	theme	starch	1047:1052	arg1	diets					1054:1058	low protein high starch diets	1030:1058	low protein high starch diets	1030:1058	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	8	76	theme	plasma	1543:1548	arg1	level					1558:1562	fasting plasma glucose level	1535:1562	fasting plasma glucose level	1535:1562	Though fasting plasma glucose level was comparable, treatment P42S18 performed inferior glucose tolerance to treatment P49S9.
36508948	9	77	theme	Nick-End	1699:1706	arg1	TUNEL					1718:1722	TUNEL	1718:1722	TUNEL	1718:1722	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	9	77	theme	Nick-End	1699:1706	arg1	Labeling					1708:1715	TdT-mediated dUTP Nick-End Labeling	1681:1715	TdT-mediated dUTP Nick-End Labeling (TUNEL) staining	1681:1732	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	8	78	theme	glucose	1616:1622	arg1	tolerance					1624:1632	inferior glucose tolerance	1607:1632	inferior glucose tolerance to treatment P49S9	1607:1651	Though fasting plasma glucose level was comparable, treatment P42S18 performed inferior glucose tolerance to treatment P49S9.
36508948	7	79	dep	representative	1469:1482	arg1	down-regulated					1511:1524	down-regulated	1511:1524	down-regulated	1511:1524	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	5	80	theme	treatment	925:933	arg1	P49S9					935:939	treatment P49S9	925:939	treatment P49S9	925:939	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	6	81	theme	protein	1034:1040	arg1	diets					1054:1058	low protein high starch diets	1030:1058	low protein high starch diets	1030:1058	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	0	82	from	salmoides	146:154	arg1	mechanism					60:68	the molecular mechanism	46:68	the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	46:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	2	83	theme	isolipidic	365:374	arg1	diets					393:397	Two isolipidic and isoenergetic diets	361:397	Two isolipidic and isoenergetic diets	361:397	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	1	84	theme	regulatory	242:251	arg1	mechanism					253:261	the regulatory mechanism	238:261	the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets	238:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	7	85	theme	lipid	1189:1193	arg1	level					1195:1199	Lower liver lipid level	1177:1199	Lower liver lipid level	1177:1199	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	13	86	theme	inflammatory	2504:2515	arg1	factors					2517:2523	these inflammatory factors	2498:2523	these inflammatory factors	2498:2523	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	7	87	theme	synthesis	1408:1416	arg1	representative					1377:1390	representative	1377:1390	representative	1377:1390	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	7	88	theme	Lower	1177:1181	arg1	level					1195:1199	Lower liver lipid level	1177:1199	Lower liver lipid level	1177:1199	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	11	89	theme	PI3K/Akt	2291:2298	arg1	pathway					2307:2313	PI3K/Akt signal pathway	2291:2313	PI3K/Akt signal pathway	2291:2313	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	8	90	theme	treatment	1580:1588	arg1	P42S18					1590:1595	treatment P42S18	1580:1595	treatment P42S18	1580:1595	Though fasting plasma glucose level was comparable, treatment P42S18 performed inferior glucose tolerance to treatment P49S9.
36508948	3	91	theme	body	625:628	arg1	weight					630:635	initial body weight	617:635	initial body weight	617:635	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	3	91	theme	body	625:628	arg1	4.65 ± 0.01 g					638:650	4.65 ± 0.01 g	638:650	4.65 ± 0.01 g	638:650	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	1	92	theme	injury	272:277	arg1	mechanism					253:261	the regulatory mechanism	238:261	the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets	238:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	14	93	theme	caspase	2851:2857	arg1	expression					2837:2846	the expression	2833:2846	the expression of caspase 8 and cytochrome c	2833:2876	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	14	94	theme	cytochrome	2865:2874	arg1	c					2876:2876	cytochrome c	2865:2876	cytochrome c	2865:2876	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	9	95	theme	LMB	1880:1882	arg1	hepatocytes					1865:1875	the hepatocytes	1861:1875	the hepatocytes of LMB	1861:1882	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	11	96	theme	AGEs	2233:2236	arg1	accumulation					2217:2228	the accumulation	2213:2228	the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway	2213:2313	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	10	97	theme	pathways	1903:1910	arg1	analysis					1912:1919	KEGG pathways analysis	1898:1919	KEGG pathways analysis	1898:1919	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	10	98	dep	/RAGE	2045:2049	arg1	receptor					2052:2059	receptor	2052:2059	receptor for AGE	2052:2067	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	0	99	theme	Micropterus	134:144	arg1	salmoides					146:154	juvenile largemouth bass Micropterus salmoides	109:154	juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	109:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	7	100	theme	hepatic	1312:1318	arg1	metabolism					1333:1342	hepatic glycerolipid metabolism	1312:1342	hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated)	1312:1525	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	10	101	theme	apoptosis	2094:2102	arg1	pathways					2114:2121	apoptosis signaling pathways	2094:2121	apoptosis signaling pathways	2094:2121	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	14	102	theme	TNF-α	2709:2713	arg1	transduction					2722:2733	TNF-α signal transduction	2709:2733	TNF-α signal transduction	2709:2733	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	9	103	theme	TdT-mediated	1681:1692	arg1	TUNEL					1718:1722	TUNEL	1718:1722	TUNEL	1718:1722	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	9	103	theme	TdT-mediated	1681:1692	arg1	Labeling					1708:1715	TdT-mediated dUTP Nick-End Labeling	1681:1715	TdT-mediated dUTP Nick-End Labeling (TUNEL) staining	1681:1732	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	6	104	theme	debranching	1157:1167	arg1	enzyme					1169:1174	the glycogenolytic glycogen debranching enzyme	1129:1174	the glycogenolytic glycogen debranching enzyme	1129:1174	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	0	105	theme	protein	164:170	arg1	diets					184:188	low protein high starch diets	160:188	low protein high starch diets	160:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	5	106	theme	efficiency	836:845	arg1	ratio					847:851	feeding efficiency ratio	828:851	feeding efficiency ratio	828:851	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	2	107	theme	%	536:536	arg1	protein					538:544	42.4 % protein and 18.2 % starch	531:562	protein	538:544	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	6	108	theme	glycogenolytic	1133:1146	arg1	enzyme					1169:1174	the glycogenolytic glycogen debranching enzyme	1129:1174	the glycogenolytic glycogen debranching enzyme	1129:1174	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	0	109	theme	starch	177:182	arg1	diets					184:188	low protein high starch diets	160:188	low protein high starch diets	160:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	3	110	theme	Each	566:569	arg1	diet					571:574	Each diet	566:574	Each diet	566:574	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	0	111	theme	liver	73:77	arg1	inflammation					79:90	liver inflammation	73:90	liver inflammation	73:90	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	5	112	theme	protein	806:812	arg1	requirements					814:825	their protein requirements	800:825	their protein requirements	800:825	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	6	113	theme	acid-Schiff	951:961	arg1	staining					969:976	Periodic acid-Schiff (PAS) staining	942:976	Periodic acid-Schiff (PAS) staining	942:976	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	6	114	theme	down-regulated	1100:1113	arg1	expression					1115:1124	down-regulated expression	1100:1124	down-regulated expression of the glycogenolytic glycogen debranching enzyme	1100:1174	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	10	115	theme	genes	1986:1990	arg1	many					1936:1939	many	1936:1939	many	1936:1939	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	10	115	theme	genes	1986:1990	arg1	genes					1986:1990	the up-regulated differentially expressed genes	1944:1990	the up-regulated differentially expressed genes	1944:1990	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	1	116	theme	protein	334:340	arg1	diets					354:358	low protein high starch diets	330:358	low protein high starch diets	330:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	11	117	theme	high	2181:2184	arg1	diets					2193:2197	low protein high starch diets	2169:2197	low protein high starch diets	2169:2197	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	10	118	theme	advanced	2014:2021	arg1	product					2037:2043	advanced glycation end product	2014:2043	advanced glycation end product	2014:2043	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	10	118	theme	advanced	2014:2021	arg1	AGE					2009:2011	AGE	2009:2011	AGE (advanced glycation end product)/RAGE (receptor for AGE)	2009:2068	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	1	119	theme	starch	347:352	arg1	diets					354:358	low protein high starch diets	330:358	low protein high starch diets	330:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	5	120	theme	treatment	893:901	arg1	P42S18					903:908	treatment P42S18	893:908	treatment P42S18	893:908	The results indicated that though the P42S18 fish up-regulated the feeding ratio to meet their protein requirements, feeding efficiency ratio and growth performance were impaired in treatment P42S18 as compared to treatment P49S9.
36508948	0	121	from	mechanism	60:68	arg1	salmoides					146:154	juvenile largemouth bass Micropterus salmoides	109:154	juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	109:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	4	122	theme	visual	681:686	arg1	satiation					688:696	visual satiation	681:696	visual satiation	681:696	Fish were fed to visual satiation for 8 weeks.
36508948	10	123	theme	AGE	2009:2011	arg1	/RAGE					2045:2049	AGE (advanced glycation end product)/RAGE	2009:2049	AGE (advanced glycation end product)/RAGE (receptor for AGE)	2009:2068	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	0	124	theme	largemouth	118:127	arg1	salmoides					146:154	juvenile largemouth bass Micropterus salmoides	109:154	juvenile largemouth bass Micropterus salmoides fed low protein high starch diets	109:188	Transcriptome analysis provides insights into the molecular mechanism of liver inflammation and apoptosis in juvenile largemouth bass Micropterus salmoides fed low protein high starch diets.
36508948	14	125	theme	receptor	2783:2790	arg1	pathway					2808:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	9	126	theme	protein	1761:1767	arg1	diets					1781:1785	low protein high starch diets	1757:1785	low protein high starch diets	1757:1785	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	6	127	dep	showed	978:983	arg1	accumulated					994:1004	accumulated	994:1004	showed glycogen accumulated in the liver of LMB fed low protein high starch diets	978:1058	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	15	128	theme	high	2948:2951	arg1	diets					2960:2964	high starch diets	2948:2964	high starch diets	2948:2964	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	13	129	theme	Toll-like	2619:2627	arg1	pathway					2648:2654	Toll-like receptor signaling pathway	2619:2654	Toll-like receptor signaling pathway	2619:2654	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	13	130	theme	natural	2570:2576	arg1	cells					2585:2589	natural killer cells	2570:2589	natural killer cells	2570:2589	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	7	131	theme	starch	1246:1251	arg1	diets					1253:1257	low protein high starch diets	1229:1257	low protein high starch diets	1229:1257	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	9	132	theme	starch	1774:1779	arg1	diets					1781:1785	low protein high starch diets	1757:1785	low protein high starch diets	1757:1785	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	1	133	theme	bass	293:296	arg1	LMB					321:323	LMB	321:323	LMB	321:323	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	1	133	theme	bass	293:296	arg1	salmoides					310:318	largemouth bass Micropterus salmoides	282:318	largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets	282:358	The present study was conducted to investigate the regulatory mechanism of liver injury in largemouth bass Micropterus salmoides (LMB) fed low protein high starch diets.
36508948	7	134	theme	protein	1233:1239	arg1	diets					1253:1257	low protein high starch diets	1229:1257	low protein high starch diets	1229:1257	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	12	135	theme	tumor	2432:2436	arg1	necrosis					2438:2445	tumor necrosis factor-α	2432:2454	tumor necrosis factor-α (TNF-α)	2432:2462	The activation of Akt induced NF-κB translocation into the nucleus thus releasing proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8.
36508948	12	135	theme	tumor	2432:2436	arg1	TNF-α					2457:2461	TNF-α	2457:2461	TNF-α	2457:2461	The activation of Akt induced NF-κB translocation into the nucleus thus releasing proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8.
36508948	2	136	theme	different	420:428	arg1	protein					430:436	different protein and starch ratios	420:454	protein	430:436	Two isolipidic and isoenergetic diets were formulated with different protein and starch ratios, being named as diets P49S9 (48.8 % protein and 9.06 % starch) and P42S18 (42.4 % protein and 18.2 % starch).
36508948	13	137	theme	T	2547:2547	arg1	stimulation					2554:2564	T cell stimulation	2547:2564	T cell stimulation	2547:2564	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	7	138	theme	breakdown	1500:1508	arg1	representative					1469:1482	representative	1469:1482	representative	1469:1482	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	7	139	from	changes	1301:1307	arg1	metabolism					1333:1342	hepatic glycerolipid metabolism	1312:1342	hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated)	1312:1525	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	11	140	theme	low	2169:2171	arg1	diets					2193:2197	low protein high starch diets	2169:2197	low protein high starch diets	2169:2197	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	8	141	theme	fasting	1535:1541	arg1	level					1558:1562	fasting plasma glucose level	1535:1562	fasting plasma glucose level	1535:1562	Though fasting plasma glucose level was comparable, treatment P42S18 performed inferior glucose tolerance to treatment P49S9.
36508948	13	142	theme	receptor	2629:2636	arg1	pathway					2648:2654	Toll-like receptor signaling pathway	2619:2654	Toll-like receptor signaling pathway	2619:2654	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	7	143	dep	representative	1377:1390	arg1	up-regulated					1419:1430	up-regulated	1419:1430	up-regulated	1419:1430	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	14	144	theme	signal	2715:2720	arg1	transduction					2722:2733	TNF-α signal transduction	2709:2733	TNF-α signal transduction	2709:2733	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	9	145	theme	integrity	1820:1828	arg1	apoptosis					1848:1856	apoptosis	1848:1856	apoptosis	1848:1856	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	9	145	theme	integrity	1820:1828	arg1	inflammation					1831:1842	inflammation	1831:1842	inflammation	1831:1842	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	9	145	theme	integrity	1820:1828	arg1	disruption					1795:1804	disruption	1795:1804	disruption of structural integrity	1795:1828	Hematoxylin-eosin (HE) and TdT-mediated dUTP Nick-End Labeling (TUNEL) staining suggested that feeding low protein high starch diets induced disruption of structural integrity, inflammation and apoptosis in the hepatocytes of LMB.
36508948	8	146	theme	glucose	1550:1556	arg1	level					1558:1562	fasting plasma glucose level	1535:1562	fasting plasma glucose level	1535:1562	Though fasting plasma glucose level was comparable, treatment P42S18 performed inferior glucose tolerance to treatment P49S9.
36508948	13	147	theme	signaling	2638:2646	arg1	pathway					2648:2654	Toll-like receptor signaling pathway	2619:2654	Toll-like receptor signaling pathway	2619:2654	The release of these inflammatory factors concomitantly induced T cell stimulation and natural killer cells chemotactic effects through Toll-like receptor signaling pathway.
36508948	3	148	theme	LMB	612:614	arg1	juveniles					653:661	LMB (initial body weight, 4.65 ± 0.01 g) juveniles	612:661	LMB (initial body weight, 4.65 ± 0.01 g) juveniles	612:661	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36508948	15	149	dep	hepatocytes	2974:2984	arg1	hepatocytes					2974:2984	hepatocytes inflammation and apoptosis	2974:3011	hepatocytes inflammation and apoptosis	2974:3011	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	15	149	dep	hepatocytes	2974:2984	arg1	apoptosis					3003:3011	apoptosis	3003:3011	apoptosis	3003:3011	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	15	149	dep	hepatocytes	2974:2984	arg1	inflammation					2986:2997	inflammation	2986:2997	inflammation	2986:2997	In conclusion, our results demonstrated that feeding low protein and high starch diets induced hepatocytes inflammation and apoptosis in LMB through the PI3K/Akt/NF-κB signaling pathway.
36508948	6	150	theme	high	1042:1045	arg1	diets					1054:1058	low protein high starch diets	1030:1058	low protein high starch diets	1030:1058	Periodic acid-Schiff (PAS) staining showed glycogen accumulated in the liver of LMB fed low protein high starch diets, and the reason should be attributed to down-regulated expression of the glycogenolytic glycogen debranching enzyme.
36508948	12	151	theme	NF-κB	2346:2350	arg1	translocation					2352:2364	NF-κB translocation	2346:2364	NF-κB translocation into the nucleus	2346:2381	The activation of Akt induced NF-κB translocation into the nucleus thus releasing proinflammatory factors including tumor necrosis factor-α (TNF-α) and interleukin-8.
36508948	8	152	theme	treatment	1637:1645	arg1	P49S9					1647:1651	treatment P49S9	1637:1651	treatment P49S9	1637:1651	Though fasting plasma glucose level was comparable, treatment P42S18 performed inferior glucose tolerance to treatment P49S9.
36508948	7	153	theme	diacylglycerol	1437:1450	arg1	acyltransferase					1452:1466	diacylglycerol acyltransferase	1437:1466	diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated)	1437:1525	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	7	154	theme	liver	1183:1187	arg1	level					1195:1199	Lower liver lipid level	1177:1199	Lower liver lipid level	1177:1199	Lower liver lipid level was associated with feeding low protein high starch diets in LMB, which should be resulted from the changes in hepatic glycerolipid metabolism regulated by lipoprotein lipase (representative of triglyceride synthesis, up-regulated) and diacylglycerol acyltransferase (representative of triglyceride breakdown, down-regulated).
36508948	10	155	theme	end	2033:2035	arg1	product					2037:2043	advanced glycation end product	2014:2043	advanced glycation end product	2014:2043	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	10	155	theme	end	2033:2035	arg1	AGE					2009:2011	AGE	2009:2011	AGE (advanced glycation end product)/RAGE (receptor for AGE)	2009:2068	As expected, KEGG pathways analysis indicated that many of the up-regulated differentially expressed genes were enriched in AGE (advanced glycation end product)/RAGE (receptor for AGE), Toll-like receptor and apoptosis signaling pathways.
36508948	14	156	theme	of	2792:2793	arg1	pathway					2808:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	the receptor of TNF (TNF-R1) pathway	2779:2814	Besides mediating inflammation and immune response, TNF-α signal transduction participated in mediating apoptosis through the receptor of TNF (TNF-R1) pathway by up-regulating the expression of caspase 8 and cytochrome c.
36508948	11	157	theme	transcriptome	2128:2140	arg1	data					2142:2145	Our transcriptome data	2124:2145	Our transcriptome data	2124:2145	Our transcriptome data revealed that feeding low protein high starch diets might promote the accumulation of AGEs in LMB, which bound to RAGE and subsequently induced PI3K/Akt signal pathway.
36508948	3	158	dep	triplicate	587:596	arg1	replicates					598:607	replicates	598:607	replicates	598:607	Each diet was fed to triplicate replicates of LMB (initial body weight, 4.65 ± 0.01 g) juveniles.
36838515	0	0	theme	Saussurea	84:92	arg1	pulchella					94:102	Saussurea pulchella	84:102	Saussurea pulchella	84:102	Study on the Comprehensive Phytochemicals and the Anti-Ulcerative Colitis Effect of Saussurea pulchella.
36838515	5	1	theme	sodium	619:624	arg1	sulfate					626:632	dextran sodium sulfate	611:632	a dextran sodium sulfate (DSS) model of UC mice	609:655	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	5	1	theme	sodium	619:624	arg1	DSS					635:637	DSS	635:637	DSS	635:637	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	1	2	theme	traditional	146:156	arg1	plant					168:172	a traditional medicinal plant	144:172	a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects	144:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	2	theme	traditional	146:156	arg1	pulchella					126:134	BACKGROUND Saussurea pulchella	105:134	BACKGROUND Saussurea pulchella (SP)	105:139	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	3	theme	biological	234:243	arg1	effects					292:298	its anti-inflammatory effects	270:298	particularly its anti-inflammatory effects	257:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	3	theme	biological	234:243	arg1	activities					245:254	its diverse biological activities	222:254	its diverse biological activities	222:254	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	10	4	theme	UC	1534:1535	arg1	progression					1519:1529	the progression	1515:1529	the progression of UC	1515:1535	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	5	5	theme	sulfate	626:632	arg1	model					640:644	a dextran sodium sulfate (DSS) model	609:644	a dextran sodium sulfate (DSS) model of UC mice	609:655	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	1	6	theme	medicinal	158:166	arg1	plant					168:172	a traditional medicinal plant	144:172	a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects	144:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	6	theme	medicinal	158:166	arg1	pulchella					126:134	BACKGROUND Saussurea pulchella	105:134	BACKGROUND Saussurea pulchella (SP)	105:139	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	4	7	theme	%	551:551	arg1	extract					561:567	SP 75% ethanol extract	546:567	SP 75% ethanol extract	546:567	METHODS First, qualitative and quantitative analysis of SP 75% ethanol extract was performed by UPLC-Q/TOF-MS.
36838515	10	8	theme	CONCLUSIONS	1452:1462	arg1	SP					1464:1465	CONCLUSIONS SP	1452:1465	CONCLUSIONS SP 75% ethanol extract	1452:1485	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	2	9	theme	ulcerative	342:351	arg1	colitis					353:359	ulcerative colitis	342:359	ulcerative colitis (UC)	342:364	However, the alleviation effect of SP on ulcerative colitis (UC) has not yet been realized.
36838515	2	9	theme	ulcerative	342:351	arg1	UC					362:363	UC	362:363	UC	362:363	However, the alleviation effect of SP on ulcerative colitis (UC) has not yet been realized.
36838515	2	10	from	effect	326:331	arg1	colitis					353:359	ulcerative colitis	342:359	ulcerative colitis (UC)	342:364	However, the alleviation effect of SP on ulcerative colitis (UC) has not yet been realized.
36838515	2	10	from	effect	326:331	arg1	UC					362:363	UC	362:363	UC	362:363	However, the alleviation effect of SP on ulcerative colitis (UC) has not yet been realized.
36838515	6	11	theme	related	945:951	arg1	metabolites					889:899	the key metabolites	881:899	the key metabolites	881:899	Third, an integration of network pharmacology with metabolomics was performed to investigate the key metabolites, biological targets and metabolisms closely related to the effect of SP.
36838515	0	12	theme	pulchella	94:102	arg1	Effect					74:79	the Anti-Ulcerative Colitis Effect	46:79	the Anti-Ulcerative Colitis Effect of Saussurea pulchella	46:102	Study on the Comprehensive Phytochemicals and the Anti-Ulcerative Colitis Effect of Saussurea pulchella.
36838515	0	12	theme	pulchella	94:102	arg1	Phytochemicals					27:40	the Comprehensive Phytochemicals	9:40	the Comprehensive Phytochemicals	9:40	Study on the Comprehensive Phytochemicals and the Anti-Ulcerative Colitis Effect of Saussurea pulchella.
36838515	6	13	theme	key	885:887	arg1	metabolites					889:899	the key metabolites	881:899	the key metabolites	881:899	Third, an integration of network pharmacology with metabolomics was performed to investigate the key metabolites, biological targets and metabolisms closely related to the effect of SP.
36838515	8	14	theme	GSH	1244:1246	arg1	level					1248:1252	GSH level	1244:1252	GSH level	1244:1252	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	4	15	theme	extract	561:567	arg1	analysis					534:541	qualitative and quantitative analysis	505:541	qualitative and quantitative analysis of SP 75% ethanol extract	505:567	METHODS First, qualitative and quantitative analysis of SP 75% ethanol extract was performed by UPLC-Q/TOF-MS.
36838515	8	16	theme	MDA	1212:1214	arg1	score					1141:1145	the DAI score	1133:1145	the DAI score	1133:1145	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	16	theme	MDA	1212:1214	arg1	coefficient					1155:1165	spleen coefficient	1148:1165	spleen coefficient	1148:1165	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	16	theme	MDA	1212:1214	arg1	levels					1175:1180	the levels	1171:1180	the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity;	1171:1270	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	17	theme	SOD	1258:1260	arg1	activity					1262:1269	SOD activity	1258:1269	SOD activity	1258:1269	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	18	from	barrier	1299:1305	arg1	mice					1317:1320	the UC mice	1310:1320	the UC mice	1310:1320	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	19	theme	TNF-α	1185:1189	arg1	score					1141:1145	the DAI score	1133:1145	the DAI score	1133:1145	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	19	theme	TNF-α	1185:1189	arg1	coefficient					1155:1165	spleen coefficient	1148:1165	spleen coefficient	1148:1165	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	19	theme	TNF-α	1185:1189	arg1	levels					1175:1180	the levels	1171:1180	the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity;	1171:1270	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	9	20	theme	biomarkers,18	1347:1359	arg1	targets					1361:1367	10 metabolite biomarkers,18 targets	1333:1367	10 metabolite biomarkers,18 targets	1333:1367	Moreover, 10 metabolite biomarkers,18 targets and 5 metabolisms were found to play crucial roles in the treatment of UC with SP.
36838515	6	21	theme	pharmacology	821:832	arg1	integration					798:808	an integration	795:808	an integration of network pharmacology with metabolomics	795:850	Third, an integration of network pharmacology with metabolomics was performed to investigate the key metabolites, biological targets and metabolisms closely related to the effect of SP.
36838515	4	22	theme	ethanol	553:559	arg1	extract					561:567	SP 75% ethanol extract	546:567	SP 75% ethanol extract	546:567	METHODS First, qualitative and quantitative analysis of SP 75% ethanol extract was performed by UPLC-Q/TOF-MS.
36838515	8	23	theme	DAI	1137:1139	arg1	score					1141:1145	the DAI score	1133:1145	the DAI score	1133:1145	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	3	24	theme	chemical	420:427	arg1	composition					429:439	chemical composition	420:439	chemical composition	420:439	PURPOSE To investigate the chemical composition and therapeutic effect of SP extract against UC.
36838515	5	25	theme	oxidative	737:745	arg1	stress					747:752	oxidative stress	737:752	oxidative stress indexes	737:760	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	6	26	theme	network	813:819	arg1	pharmacology					821:832	network pharmacology	813:832	network pharmacology	813:832	Third, an integration of network pharmacology with metabolomics was performed to investigate the key metabolites, biological targets and metabolisms closely related to the effect of SP.
36838515	10	27	theme	%	1469:1469	arg1	extract					1479:1485	75% ethanol extract	1467:1485	CONCLUSIONS SP 75% ethanol extract	1452:1485	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	9	28	theme	UC	1440:1441	arg1	treatment					1427:1435	the treatment	1423:1435	the treatment of UC with SP	1423:1449	Moreover, 10 metabolite biomarkers,18 targets and 5 metabolisms were found to play crucial roles in the treatment of UC with SP.
36838515	2	29	theme	alleviation	314:324	arg1	effect					326:331	the alleviation effect	310:331	the alleviation effect of SP on ulcerative colitis (UC)	310:364	However, the alleviation effect of SP on ulcerative colitis (UC) has not yet been realized.
36838515	10	30	theme	75	1467:1468	arg1	%					1469:1469	%	1469:1469	%	1469:1469	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	8	31	theme	IL-6	1192:1195	arg1	score					1141:1145	the DAI score	1133:1145	the DAI score	1133:1145	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	31	theme	IL-6	1192:1195	arg1	coefficient					1155:1165	spleen coefficient	1148:1165	spleen coefficient	1148:1165	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	31	theme	IL-6	1192:1195	arg1	levels					1175:1180	the levels	1171:1180	the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity;	1171:1270	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	5	32	theme	stress	747:752	arg1	indexes					754:760	oxidative stress indexes	737:760	oxidative stress indexes	737:760	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	5	33	theme	dextran	611:617	arg1	sulfate					626:632	dextran sodium sulfate	611:632	a dextran sodium sulfate (DSS) model of UC mice	609:655	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	5	33	theme	dextran	611:617	arg1	DSS					635:637	DSS	635:637	DSS	635:637	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	6	34	with	integration	798:808	arg1	metabolomics					839:850	metabolomics	839:850	metabolomics	839:850	Third, an integration of network pharmacology with metabolomics was performed to investigate the key metabolites, biological targets and metabolisms closely related to the effect of SP.
36838515	0	35	theme	Comprehensive	13:25	arg1	Phytochemicals					27:40	the Comprehensive Phytochemicals	9:40	the Comprehensive Phytochemicals	9:40	Study on the Comprehensive Phytochemicals and the Anti-Ulcerative Colitis Effect of Saussurea pulchella.
36838515	1	36	theme	anti-inflammatory	274:290	arg1	effects					292:298	its anti-inflammatory effects	270:298	particularly its anti-inflammatory effects	257:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	36	theme	anti-inflammatory	274:290	arg1	activities					245:254	its diverse biological activities	222:254	its diverse biological activities	222:254	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	5	37	theme	mice	652:655	arg1	model					640:644	a dextran sodium sulfate (DSS) model	609:644	a dextran sodium sulfate (DSS) model of UC mice	609:655	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	1	38	theme	BACKGROUND	105:114	arg1	SP					137:138	SP	137:138	SP	137:138	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	38	theme	BACKGROUND	105:114	arg1	pulchella					126:134	BACKGROUND Saussurea pulchella	105:134	BACKGROUND Saussurea pulchella (SP)	105:139	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	38	theme	BACKGROUND	105:114	arg1	plant					168:172	a traditional medicinal plant	144:172	a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects	144:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	8	39	theme	intestinal	1288:1297	arg1	barrier					1299:1305	the intestinal barrier	1284:1305	the intestinal barrier in the UC mice	1284:1320	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	3	40	theme	therapeutic	445:455	arg1	effect					457:462	therapeutic effect	445:462	therapeutic effect	445:462	PURPOSE To investigate the chemical composition and therapeutic effect of SP extract against UC.
36838515	4	41	theme	75	549:550	arg1	%					551:551	%	551:551	%	551:551	METHODS First, qualitative and quantitative analysis of SP 75% ethanol extract was performed by UPLC-Q/TOF-MS.
36838515	8	42	theme	iNOS	1198:1201	arg1	score					1141:1145	the DAI score	1133:1145	the DAI score	1133:1145	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	42	theme	iNOS	1198:1201	arg1	coefficient					1155:1165	spleen coefficient	1148:1165	spleen coefficient	1148:1165	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	42	theme	iNOS	1198:1201	arg1	levels					1175:1180	the levels	1171:1180	the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity;	1171:1270	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	5	43	from	effects	684:690	arg1	factors					728:734	inflammatory factors	715:734	inflammatory factors	715:734	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	5	43	from	effects	684:690	arg1	indexes					754:760	oxidative stress indexes	737:760	oxidative stress indexes	737:760	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	5	43	from	effects	684:690	arg1	histopathology					772:785	colon histopathology	766:785	colon histopathology	766:785	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	5	43	from	effects	684:690	arg1	symptoms					705:712	the symptoms	701:712	the symptoms	701:712	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	1	44	theme	Saussurea	116:124	arg1	SP					137:138	SP	137:138	SP	137:138	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	44	theme	Saussurea	116:124	arg1	pulchella					126:134	BACKGROUND Saussurea pulchella	105:134	BACKGROUND Saussurea pulchella (SP)	105:139	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	44	theme	Saussurea	116:124	arg1	plant					168:172	a traditional medicinal plant	144:172	a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects	144:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	10	45	dep	alleviate	1505:1513	arg1	classified					1562:1571	classified	1562:1571	could be classified as a novel natural treatment for UC	1553:1607	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	4	46	theme	SP	546:547	arg1	extract					561:567	SP 75% ethanol extract	546:567	SP 75% ethanol extract	546:567	METHODS First, qualitative and quantitative analysis of SP 75% ethanol extract was performed by UPLC-Q/TOF-MS.
36838515	7	47	theme	ethanol	994:1000	arg1	extract					1002:1008	the SP ethanol extract	987:1008	the SP ethanol extract	987:1008	RESULTS From the SP ethanol extract, 149 compounds were identified qualitatively and 20 were determined quantitatively.
36838515	7	47	theme	ethanol	994:1000	arg1	compounds					1015:1023	149 compounds	1011:1023	149 compounds	1011:1023	RESULTS From the SP ethanol extract, 149 compounds were identified qualitatively and 20 were determined quantitatively.
36838515	8	48	theme	MPO	1204:1206	arg1	score					1141:1145	the DAI score	1133:1145	the DAI score	1133:1145	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	48	theme	MPO	1204:1206	arg1	coefficient					1155:1165	spleen coefficient	1148:1165	spleen coefficient	1148:1165	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	48	theme	MPO	1204:1206	arg1	levels					1175:1180	the levels	1171:1180	the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity;	1171:1270	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	6	49	theme	biological	902:911	arg1	targets					913:919	biological targets	902:919	biological targets	902:919	Third, an integration of network pharmacology with metabolomics was performed to investigate the key metabolites, biological targets and metabolisms closely related to the effect of SP.
36838515	9	50	theme	metabolite	1336:1345	arg1	targets					1361:1367	10 metabolite biomarkers,18 targets	1333:1367	10 metabolite biomarkers,18 targets	1333:1367	Moreover, 10 metabolite biomarkers,18 targets and 5 metabolisms were found to play crucial roles in the treatment of UC with SP.
36838515	1	51	theme	folk	197:200	arg1	medicine					202:209	folk medicine	197:209	folk medicine	197:209	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	5	52	theme	inflammatory	715:726	arg1	factors					728:734	inflammatory factors	715:734	inflammatory factors	715:734	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	4	53	theme	qualitative	505:515	arg1	analysis					534:541	qualitative and quantitative analysis	505:541	qualitative and quantitative analysis of SP 75% ethanol extract	505:567	METHODS First, qualitative and quantitative analysis of SP 75% ethanol extract was performed by UPLC-Q/TOF-MS.
36838515	7	54	from	extract	1002:1008	arg1	RESULTS					974:980	RESULTS	974:980	RESULTS From the SP ethanol extract, 149 compounds	974:1023	RESULTS From the SP ethanol extract, 149 compounds were identified qualitatively and 20 were determined quantitatively.
36838515	8	55	theme	spleen	1148:1153	arg1	coefficient					1155:1165	spleen coefficient	1148:1165	spleen coefficient	1148:1165	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	0	56	theme	Anti-Ulcerative	50:64	arg1	Effect					74:79	the Anti-Ulcerative Colitis Effect	46:79	the Anti-Ulcerative Colitis Effect of Saussurea pulchella	46:102	Study on the Comprehensive Phytochemicals and the Anti-Ulcerative Colitis Effect of Saussurea pulchella.
36838515	10	57	theme	natural	1584:1590	arg1	treatment					1592:1600	a novel natural treatment	1576:1600	a novel natural treatment for UC	1576:1607	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	3	58	theme	extract	470:476	arg1	composition					429:439	chemical composition	420:439	chemical composition	420:439	PURPOSE To investigate the chemical composition and therapeutic effect of SP extract against UC.
36838515	3	58	theme	extract	470:476	arg1	effect					457:462	therapeutic effect	445:462	therapeutic effect	445:462	PURPOSE To investigate the chemical composition and therapeutic effect of SP extract against UC.
36838515	9	59	with	treatment	1427:1435	arg1	SP					1448:1449	SP	1448:1449	SP	1448:1449	Moreover, 10 metabolite biomarkers,18 targets and 5 metabolisms were found to play crucial roles in the treatment of UC with SP.
36838515	4	60	theme	quantitative	521:532	arg1	analysis					534:541	qualitative and quantitative analysis	505:541	qualitative and quantitative analysis of SP 75% ethanol extract	505:567	METHODS First, qualitative and quantitative analysis of SP 75% ethanol extract was performed by UPLC-Q/TOF-MS.
36838515	7	61	theme	SP	991:992	arg1	extract					1002:1008	the SP ethanol extract	987:1008	the SP ethanol extract	987:1008	RESULTS From the SP ethanol extract, 149 compounds were identified qualitatively and 20 were determined quantitatively.
36838515	7	61	theme	SP	991:992	arg1	compounds					1015:1023	149 compounds	1011:1023	149 compounds	1011:1023	RESULTS From the SP ethanol extract, 149 compounds were identified qualitatively and 20 were determined quantitatively.
36838515	1	62	used	used	189:192	arg2	plant					168:172	a traditional medicinal plant	144:172	a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects	144:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	62	used	used	189:192	arg2	pulchella					126:134	BACKGROUND Saussurea pulchella	105:134	BACKGROUND Saussurea pulchella (SP)	105:139	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	2	63	theme	SP	336:337	arg1	effect					326:331	the alleviation effect	310:331	the alleviation effect of SP on ulcerative colitis (UC)	310:364	However, the alleviation effect of SP on ulcerative colitis (UC) has not yet been realized.
36838515	8	64	dep	increase	1217:1224	arg1	length					1236:1241	colon length	1230:1241	colon length	1230:1241	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	64	dep	increase	1217:1224	arg1	level					1248:1252	GSH level	1244:1252	GSH level	1244:1252	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	64	dep	increase	1217:1224	arg1	activity					1262:1269	SOD activity	1258:1269	SOD activity	1258:1269	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	8	65	theme	UC	1314:1315	arg1	mice					1317:1320	the UC mice	1310:1320	the UC mice	1310:1320	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	3	66	dep	composition	429:439	arg1	the					416:418	the	416:418	the	416:418	PURPOSE To investigate the chemical composition and therapeutic effect of SP extract against UC.
36838515	8	67	dep	TNF-α	1185:1189	arg1	increase					1217:1224	increase the colon length, GSH level and SOD activity	1217:1269	TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity;	1185:1270	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	10	68	theme	novel	1578:1582	arg1	treatment					1592:1600	a novel natural treatment	1576:1600	a novel natural treatment for UC	1576:1607	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	5	69	theme	UC	649:650	arg1	mice					652:655	UC mice	649:655	UC mice	649:655	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	3	70	theme	SP	467:468	arg1	extract					470:476	SP extract	467:476	SP extract	467:476	PURPOSE To investigate the chemical composition and therapeutic effect of SP extract against UC.
36838515	10	71	dep	SP	1464:1465	arg1	extract					1479:1485	75% ethanol extract	1467:1485	CONCLUSIONS SP 75% ethanol extract	1452:1485	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	6	72	theme	SP	970:971	arg1	effect					960:965	the effect	956:965	the effect of SP	956:971	Third, an integration of network pharmacology with metabolomics was performed to investigate the key metabolites, biological targets and metabolisms closely related to the effect of SP.
36838515	5	73	theme	SP	695:696	arg1	effects					684:690	the effects	680:690	the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology	680:785	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	5	74	theme	colon	766:770	arg1	histopathology					772:785	colon histopathology	766:785	colon histopathology	766:785	Second, a dextran sodium sulfate (DSS) model of UC mice was developed to study the effects of SP on the symptoms, inflammatory factors, oxidative stress indexes and colon histopathology.
36838515	8	75	theme	colon	1230:1234	arg1	length					1236:1241	colon length	1230:1241	colon length	1230:1241	The SP could dose-dependently decrease the DAI score, spleen coefficient and the levels of TNF-α, IL-6, iNOS, MPO and MDA; increase the colon length, GSH level and SOD activity; and protect the intestinal barrier in the UC mice.
36838515	0	76	theme	Colitis	66:72	arg1	Effect					74:79	the Anti-Ulcerative Colitis Effect	46:79	the Anti-Ulcerative Colitis Effect of Saussurea pulchella	46:102	Study on the Comprehensive Phytochemicals and the Anti-Ulcerative Colitis Effect of Saussurea pulchella.
36838515	10	77	theme	ethanol	1471:1477	arg1	extract					1479:1485	75% ethanol extract	1467:1485	CONCLUSIONS SP 75% ethanol extract	1452:1485	CONCLUSIONS SP 75% ethanol extract could effectively alleviate the progression of UC and, therefore, could be classified as a novel natural treatment for UC.
36838515	9	78	theme	crucial	1406:1412	arg1	roles					1414:1418	crucial roles	1406:1418	crucial roles	1406:1418	Moreover, 10 metabolite biomarkers,18 targets and 5 metabolisms were found to play crucial roles in the treatment of UC with SP.
36838515	1	79	theme	diverse	226:232	arg1	effects					292:298	its anti-inflammatory effects	270:298	particularly its anti-inflammatory effects	257:298	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
36838515	1	79	theme	diverse	226:232	arg1	activities					245:254	its diverse biological activities	222:254	its diverse biological activities	222:254	BACKGROUND Saussurea pulchella (SP) is a traditional medicinal plant that is widely used in folk medicine because of its diverse biological activities, particularly its anti-inflammatory effects.
35975084	2	0	theme	glycopeptide	690:701	arg1	feasibility					670:680	the feasibility	666:680	the feasibility of this glycopeptide as a novel biomarker of RCC	666:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	5	1	theme	healthy	1549:1555	arg1	controls					1557:1564	healthy controls	1549:1564	healthy controls	1549:1564	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	4	2	theme	Selective	950:958	arg1	assay					980:984	Selective reaction monitoring assay	950:984	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards	950:1039	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	3	theme	monitoring	969:978	arg1	assay					980:984	Selective reaction monitoring assay	950:984	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards	950:1039	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	6	4	theme	structure-defined	1692:1708	arg1	glycopeptides					1720:1732	structure-defined synthetic glycopeptides	1692:1732	structure-defined synthetic glycopeptides	1692:1732	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	3	5	attach	attached	776:783	arg2	structure					766:774	the precise N-glycan structure	745:774	the precise N-glycan structure attached to Asn374	745:793	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	3	5	attach	attached	776:783	arg1	Asn374					788:793	Asn374	788:793	Asn374	788:793	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	6	6	gly	glycopeptide	1866:1877	arg2	glycopeptide					1866:1877	intact glycopeptide profiling	1859:1887	intact glycopeptide profiling	1859:1887	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	4	7	theme	RCC	1239:1241	arg1	patients					1243:1250	RCC patients	1239:1250	RCC patients	1239:1250	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	2	8	theme	glycopeptide	356:367	arg1	levels					334:339	levels	334:339	levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374	334:527	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	1	9	theme	various	238:244	arg1	cancer					246:251	various cancer	238:251	various cancer	238:251	Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases.
35975084	2	10	attach	derived	369:375	arg1	clusterin					389:397	plasma clusterin	382:397	plasma clusterin	382:397	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	10	attach	derived	369:375	arg2	glycopeptide					356:367	the tryptic glycopeptide	344:367	the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374	344:527	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	10	attach	derived	369:375	arg1	372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385					400:460	372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385	400:460	372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385	400:460	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	4	11	theme	calibration	1019:1029	arg1	glycopeptides					1002:1014	synthetic glycopeptides	992:1014	synthetic glycopeptides	992:1014	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	11	theme	calibration	1019:1029	arg1	standards					1031:1039	calibration standards	1019:1039	calibration standards	1019:1039	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	6	12	theme	bottom-up	1629:1637	arg1	glycoproteomics					1639:1653	the bottom-up glycoproteomics	1625:1653	the bottom-up glycoproteomics	1625:1653	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	2	13	gly	glycopeptide	690:701	arg2	glycopeptide					690:701	this glycopeptide	685:701	this glycopeptide	685:701	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	3	14	theme	Neu5Acα2,6Gal	855:867	arg1	moiety					869:874	the Neu5Acα2,6Gal moiety	851:874	the Neu5Acα2,6Gal moiety	851:874	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	3	15	gly	glycopeptides	896:908	arg2	glycopeptides					896:908	key glycopeptides	892:908	key glycopeptides having one of the two putative isomers	892:947	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	3	16	theme	putative	932:939	arg1	isomers					941:947	the two putative isomers	924:947	the two putative isomers	924:947	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	6	17	theme	intact	1859:1864	arg1	profiling					1879:1887	intact glycopeptide profiling	1859:1887	intact glycopeptide profiling	1859:1887	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	2	18	theme	cell	602:605	arg1	RCC					629:631	RCC	629:631	RCC	629:631	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	18	theme	cell	602:605	arg1	carcinoma					618:626	clear cell renal cell carcinoma	596:626	clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC	596:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	6	19	theme	present	1571:1577	arg1	study					1579:1583	The present study	1567:1583	The present study	1567:1583	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	1	20	theme	glycosylated	194:205	arg1	protein					207:213	a heavily glycosylated protein	184:213	a heavily glycosylated protein that is upregulated in various cancer and neurological diseases	184:277	Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases.
35975084	1	20	theme	glycosylated	194:205	arg1	Clusterin					171:179	Clusterin	171:179	Clusterin	171:179	Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases.
35975084	6	21	dep	identification	1756:1769	arg1	the					1742:1744	the	1742:1744	the	1742:1744	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	2	22	from	Asn374	522:527	arg1	N-glycan					490:497	a biantennary disialyl N-glycan	467:497	a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374	467:527	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	4	23	theme	controls	1264:1271	arg1	samples					1228:1234	serum samples	1222:1234	serum samples of RCC patients and healthy controls	1222:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	0	24	theme	glycopeptide	102:113	arg1	biomarkers					115:124	glycopeptide biomarkers	102:124	glycopeptide biomarkers pre-determined by bottom-up glycoproteomics	102:168	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	6	25	theme	existing	1838:1845	arg1	methods					1847:1853	the existing methods	1834:1853	the existing methods for intact glycopeptide profiling	1834:1887	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	4	26	gly	glycopeptides	1002:1014	arg2	standards					1031:1039	calibration standards	1019:1039	calibration standards	1019:1039	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	26	gly	glycopeptides	1002:1014	arg2	glycopeptides					1002:1014	synthetic glycopeptides	992:1014	synthetic glycopeptides	992:1014	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	27	theme	top-down	1050:1057	arg1	"					1075:1075	"top-down glycopeptidomics"	1049:1075	"top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides	1049:1142	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	0	28	theme	pre-determined	126:139	arg1	biomarkers					115:124	glycopeptide biomarkers	102:124	glycopeptide biomarkers pre-determined by bottom-up glycoproteomics	102:168	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	2	29	theme	cell	613:616	arg1	RCC					629:631	RCC	629:631	RCC	629:631	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	29	theme	cell	613:616	arg1	carcinoma					618:626	clear cell renal cell carcinoma	596:626	clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC	596:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	0	30	theme	bottom-up	144:152	arg1	glycoproteomics					154:168	bottom-up glycoproteomics	144:168	bottom-up glycoproteomics	144:168	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	2	31	theme	curative	571:578	arg1	nephrectomy					580:590	curative nephrectomy	571:590	curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC	571:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	6	32	theme	targets	1808:1814	arg1	identification					1756:1769	confident identification	1746:1769	confident identification	1746:1769	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	6	32	theme	targets	1808:1814	arg1	quantitation					1775:1786	quantitation	1775:1786	quantitation	1775:1786	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	5	33	from	Asn374	1486:1491	arg1	terminals					1473:1481	homo Neu5Acα2,6Gal terminals	1454:1481	homo Neu5Acα2,6Gal terminals at Asn374	1454:1491	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	34	theme	homo	1454:1457	arg1	terminals					1473:1481	homo Neu5Acα2,6Gal terminals	1454:1481	homo Neu5Acα2,6Gal terminals at Asn374	1454:1491	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	0	35	theme	Selective	0:8	arg1	monitoring					19:28	Selective reaction monitoring	0:28	Selective reaction monitoring approach	0:37	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	6	36	gly	glycopeptide	1795:1806	arg2	glycopeptide					1795:1806	glycopeptide	1795:1806	glycopeptide	1795:1806	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	0	37	theme	monitoring	19:28	arg1	approach					30:37	Selective reaction monitoring approach	0:37	Selective reaction monitoring approach	0:37	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	4	38	theme	targeted	1110:1117	arg1	glycopeptides					1130:1142	targeted label-free glycopeptides	1110:1142	targeted label-free glycopeptides	1110:1142	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	3	39	contain	having	910:915	arg2	one					917:919	one	917:919	one	917:919	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	3	39	contain	having	910:915	arg2	isomers					941:947	the two putative isomers	924:947	the two putative isomers	924:947	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	3	39	contain	having	910:915	arg1	glycopeptides					896:908	key glycopeptides	892:908	key glycopeptides having one of the two putative isomers	892:947	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	4	40	theme	glycopeptides	1130:1142	arg1	quantitation					1094:1105	the absolute quantitation	1081:1105	the absolute quantitation of targeted label-free glycopeptides	1081:1142	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	0	41	theme	synthetic	63:71	arg1	glycopeptides					73:85	structure-defined synthetic glycopeptides	45:85	structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics	45:168	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	4	42	attach	derived	1209:1215	arg2	digests					1201:1207	the complex tryptic digests	1181:1207	the complex tryptic digests derived from serum samples of RCC patients and healthy controls	1181:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	42	attach	derived	1209:1215	arg1	samples					1228:1234	serum samples	1222:1234	serum samples of RCC patients and healthy controls	1222:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	3	43	theme	N-glycan	757:764	arg1	structure					766:774	the precise N-glycan structure	745:774	the precise N-glycan structure attached to Asn374	745:793	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	2	44	dep	N-glycan	490:497	arg1	FA2G2S2					510:516	FA2G2S2	510:516	FA2G2S2	510:516	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	44	dep	N-glycan	490:497	arg1	A2G2S2					500:505	A2G2S2	500:505	A2G2S2	500:505	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	5	45	theme	RCC	1519:1521	arg1	patients					1523:1530	RCC patients	1519:1530	RCC patients	1519:1530	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	2	46	theme	plasma	382:387	arg1	clusterin					389:397	plasma clusterin	382:397	plasma clusterin	382:397	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	46	theme	plasma	382:387	arg1	372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385					400:460	372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385	400:460	372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385	400:460	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	4	47	theme	tryptic	1193:1199	arg1	digests					1201:1207	the complex tryptic digests	1181:1207	the complex tryptic digests derived from serum samples of RCC patients and healthy controls	1181:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	2	48	gly	glycopeptide	356:367	arg2	glycopeptide					356:367	the tryptic glycopeptide	344:367	the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374	344:527	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	49	theme	biantennary	469:479	arg1	N-glycan					490:497	a biantennary disialyl N-glycan	467:497	a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374	467:527	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	5	50	theme	clusterin	1337:1345	arg1	residue					1320:1326	the Asn374 residue	1309:1326	the Asn374 residue of human clusterin	1309:1345	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	50	theme	clusterin	1337:1345	arg1	clusterin					1337:1345	human clusterin	1331:1345	human clusterin	1331:1345	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	4	51	gly	glycopeptides	1130:1142	arg2	glycopeptides					1130:1142	targeted label-free glycopeptides	1110:1142	targeted label-free glycopeptides	1110:1142	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	5	52	mod	modified	1350:1357	arg3	levels					1411:1416	the levels	1407:1416	the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls	1407:1564	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	52	mod	modified	1350:1357	arg1	residue					1320:1326	the Asn374 residue	1309:1326	the Asn374 residue of human clusterin	1309:1345	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	52	mod	modified	1350:1357	arg1	clusterin					1337:1345	human clusterin	1331:1345	human clusterin	1331:1345	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	52	mod	modified	1350:1357	arg3	structure					1393:1401	the Neu5Acα2,6Gal structure	1375:1401	the Neu5Acα2,6Gal structure	1375:1401	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	53	theme	Asn374	1313:1318	arg1	residue					1320:1326	the Asn374 residue	1309:1326	the Asn374 residue of human clusterin	1309:1345	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	53	theme	Asn374	1313:1318	arg1	clusterin					1337:1345	human clusterin	1331:1345	human clusterin	1331:1345	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	4	54	theme	reaction	960:967	arg1	assay					980:984	Selective reaction monitoring assay	950:984	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards	950:1039	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	2	55	theme	novel	708:712	arg1	biomarker					714:722	a novel biomarker	706:722	a novel biomarker of RCC	706:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	4	56	theme	patients	1243:1250	arg1	samples					1228:1234	serum samples	1222:1234	serum samples of RCC patients and healthy controls	1222:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	6	57	theme	top-down	1660:1667	arg1	glycopeptidomics					1669:1684	top-down glycopeptidomics	1660:1684	top-down glycopeptidomics using structure-defined synthetic glycopeptides	1660:1732	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	2	58	theme	tryptic	348:354	arg1	glycopeptide					356:367	the tryptic glycopeptide	344:367	the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374	344:527	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	4	59	theme	serum	1222:1226	arg1	samples					1228:1234	serum samples	1222:1234	serum samples of RCC patients and healthy controls	1222:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	1	60	theme	neurological	257:268	arg1	diseases					270:277	neurological diseases	257:277	neurological diseases	257:277	Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases.
35975084	1	61	gly	glycosylated	194:205	arg1	protein					207:213	a heavily glycosylated protein	184:213	a heavily glycosylated protein that is upregulated in various cancer and neurological diseases	184:277	Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases.
35975084	1	61	gly	glycosylated	194:205	arg1	Clusterin					171:179	Clusterin	171:179	Clusterin	171:179	Clusterin is a heavily glycosylated protein that is upregulated in various cancer and neurological diseases.
35975084	6	62	theme	glycopeptide	1866:1877	arg1	profiling					1879:1887	intact glycopeptide profiling	1859:1887	intact glycopeptide profiling	1859:1887	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	5	63	theme	Neu5Acα2,6Gal	1379:1391	arg1	structure					1393:1401	the Neu5Acα2,6Gal structure	1375:1401	the Neu5Acα2,6Gal structure	1375:1401	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	2	64	theme	Iliopoulos	312:321	arg1	group					323:327	the Hancock and Iliopoulos group	296:327	the Hancock and Iliopoulos group	296:327	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	6	65	theme	confident	1746:1754	arg1	identification					1756:1769	confident identification	1746:1769	confident identification	1746:1769	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	3	66	theme	isomers	941:947	arg1	isomers					941:947	the two putative isomers	924:947	the two putative isomers	924:947	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	3	66	theme	isomers	941:947	arg1	one					917:919	one	917:919	one	917:919	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	4	67	theme	synthetic	992:1000	arg1	glycopeptides					1002:1014	synthetic glycopeptides	992:1014	synthetic glycopeptides	992:1014	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	67	theme	synthetic	992:1000	arg1	standards					1031:1039	calibration standards	1019:1039	calibration standards	1019:1039	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	68	theme	healthy	1256:1262	arg1	controls					1264:1271	healthy controls	1256:1271	healthy controls	1256:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	3	69	theme	key	892:894	arg1	glycopeptides					896:908	key glycopeptides	892:908	key glycopeptides having one of the two putative isomers	892:947	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	2	70	theme	Hancock	300:306	arg1	group					323:327	the Hancock and Iliopoulos group	296:327	the Hancock and Iliopoulos group	296:327	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	4	71	from	nM	1175:1176	arg1	digests					1201:1207	the complex tryptic digests	1181:1207	the complex tryptic digests derived from serum samples of RCC patients and healthy controls	1181:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	4	71	from	nM	1175:1176	arg1	range					1149:1153	a range	1147:1153	a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls	1147:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	2	72	theme	clear	596:600	arg1	RCC					629:631	RCC	629:631	RCC	629:631	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	72	theme	clear	596:600	arg1	carcinoma					618:626	clear cell renal cell carcinoma	596:626	clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC	596:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	6	73	theme	synthetic	1710:1718	arg1	glycopeptides					1720:1732	structure-defined synthetic glycopeptides	1692:1732	structure-defined synthetic glycopeptides	1692:1732	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	4	74	theme	absolute	1085:1092	arg1	quantitation					1094:1105	the absolute quantitation	1081:1105	the absolute quantitation of targeted label-free glycopeptides	1081:1142	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	3	75	dep	Neu5Acα2,3Gal	830:842	arg1	or/and					844:849	or/and	844:849	or/and the Neu5Acα2,6Gal moiety	844:874	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	4	76	dep	697.5	1169:1173	arg1	to					1166:1167	to	1166:1167	to	1166:1167	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	2	77	theme	carcinoma	618:626	arg1	patients					634:641	clear cell renal cell carcinoma (RCC) patients	596:641	clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC	596:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	78	theme	RCC	727:729	arg1	biomarker					714:722	a novel biomarker	706:722	a novel biomarker of RCC	706:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	6	79	theme	pre-determined	1816:1829	arg1	targets					1808:1814	the glycopeptide targets	1791:1814	the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling	1791:1887	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	0	80	gly	glycopeptide	102:113	arg2	glycopeptide					102:113	glycopeptide biomarkers	102:124	glycopeptide biomarkers pre-determined by bottom-up glycoproteomics	102:168	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	4	81	theme	glycopeptidomics	1059:1074	arg1	"					1075:1075	"top-down glycopeptidomics"	1049:1075	"top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides	1049:1142	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	0	82	theme	reaction	10:17	arg1	monitoring					19:28	Selective reaction monitoring	0:28	Selective reaction monitoring approach	0:37	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	6	83	theme	glycopeptide	1795:1806	arg1	targets					1808:1814	the glycopeptide targets	1791:1814	the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling	1791:1887	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	2	84	theme	disialyl	481:488	arg1	N-glycan					490:497	a biantennary disialyl N-glycan	467:497	a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374	467:527	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	5	85	theme	clusterin	1421:1429	arg1	structure					1393:1401	the Neu5Acα2,6Gal structure	1375:1401	the Neu5Acα2,6Gal structure	1375:1401	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	5	85	theme	clusterin	1421:1429	arg1	levels					1411:1416	the levels	1407:1416	the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls	1407:1564	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	0	86	theme	structure-defined	45:61	arg1	glycopeptides					73:85	structure-defined synthetic glycopeptides	45:85	structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics	45:168	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	2	87	theme	renal	607:611	arg1	RCC					629:631	RCC	629:631	RCC	629:631	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	2	87	theme	renal	607:611	arg1	carcinoma					618:626	clear cell renal cell carcinoma	596:626	clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC	596:729	The findings by the Hancock and Iliopoulos group that levels of the tryptic glycopeptide derived from plasma clusterin, 372Leu-Ala-Asn-Leu-Thr-Gln-Gly-Glu-Asp-Gln-Tyr-Tyr-Leu-Arg385 with a biantennary disialyl N-glycan (A2G2S2 or FA2G2S2) at Asn374 differed significantly prior to and after curative nephrectomy for clear cell renal cell carcinoma (RCC) patients motivated us to verify the feasibility of this glycopeptide as a novel biomarker of RCC.
35975084	4	88	theme	label-free	1119:1128	arg1	glycopeptides					1130:1142	targeted label-free glycopeptides	1110:1142	targeted label-free glycopeptides	1110:1142	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	3	89	theme	precise	749:755	arg1	structure					766:774	the precise N-glycan structure	745:774	the precise N-glycan structure attached to Asn374	745:793	To determine the precise N-glycan structure attached to Asn374, whether A2G2S2 is composed of the Neu5Acα2,3Gal or/and the Neu5Acα2,6Gal moiety, we synthesized key glycopeptides having one of the two putative isomers.
35975084	0	90	gly	glycopeptides	73:85	arg2	glycopeptides					73:85	structure-defined synthetic glycopeptides	45:85	structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics	45:168	Selective reaction monitoring approach using structure-defined synthetic glycopeptides for validating glycopeptide biomarkers pre-determined by bottom-up glycoproteomics.
35975084	5	91	theme	Neu5Acα2,6Gal	1459:1471	arg1	terminals					1473:1481	homo Neu5Acα2,6Gal terminals	1454:1481	homo Neu5Acα2,6Gal terminals at Asn374	1454:1491	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	6	92	theme	new	1600:1602	arg1	strategy					1604:1611	a new strategy	1598:1611	a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides	1598:1732	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35975084	5	93	theme	human	1331:1335	arg1	clusterin					1337:1345	human clusterin	1331:1345	human clusterin	1331:1345	Our results provided evidence that the Asn374 residue of human clusterin is modified dominantly with the Neu5Acα2,6Gal structure and the levels of clusterin bearing an A2G2S2 with homo Neu5Acα2,6Gal terminals at Asn374 decrease significantly in RCC patients as compared with healthy controls.
35975084	4	94	theme	complex	1185:1191	arg1	digests					1201:1207	the complex tryptic digests	1181:1207	the complex tryptic digests derived from serum samples of RCC patients and healthy controls	1181:1271	Selective reaction monitoring assay using synthetic glycopeptides as calibration standards allowed "top-down glycopeptidomics" for the absolute quantitation of targeted label-free glycopeptides in a range from 313.3 to 697.5 nM in the complex tryptic digests derived from serum samples of RCC patients and healthy controls.
35975084	6	95	gly	glycopeptides	1720:1732	arg2	glycopeptides					1720:1732	structure-defined synthetic glycopeptides	1692:1732	structure-defined synthetic glycopeptides	1692:1732	The present study elicits that a new strategy integrating the bottom-up glycoproteomics with top-down glycopeptidomics using structure-defined synthetic glycopeptides enables the confident identification and quantitation of the glycopeptide targets pre-determined by the existing methods for intact glycopeptide profiling.
35660350	10	0	theme	rDNA	1379:1382	arg1	sequencing					1384:1393	16S rDNA sequencing	1375:1393	16S rDNA sequencing technology	1375:1404	Intestinal flora changes were detected by 16S rDNA sequencing technology.
35660350	21	1	theme	equilibrium	2868:2878	arg1	levels					2880:2885	three equilibrium levels	2862:2885	three equilibrium levels	2862:2885	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	11	2	theme	Targeted	1407:1414	arg1	detection					1439:1447	Targeted short-chain fatty acid detection	1407:1447	Targeted short-chain fatty acid detection	1407:1447	Targeted short-chain fatty acid detection was performed by GC-MS/MS, and Lipopolysaccharide (LPS) levels in feces were quantified with an ELISA kit.
35660350	18	3	from	content	2362:2368	arg1	feces					2373:2377	feces	2373:2377	feces	2373:2377	In addition, berberine reduced LPS content in feces in mice with colitis-associated colorectal tumorigenesis.
35660350	1	4	theme	intestinal	278:287	arg1	flora					289:293	a large intestinal flora	270:293	a large intestinal flora	270:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	9	5	from	effect	1198:1203	arg1	tumorigenesis					1251:1263	colitis-associated colorectal tumorigenesis	1221:1263	colitis-associated colorectal tumorigenesis	1221:1263	The effect of berberine on colitis-associated colorectal tumorigenesis was assessed by general imaging, tumor counting, and Ki67 staining.
35660350	21	6	dep	acids	2950:2954	arg1	2					2929:2929	2	2929:2929	2	2929:2929	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	2	7	theme	CAC	385:387	arg1	treatment					389:397	CAC treatment	385:397	CAC treatment	385:397	The increasing role of intestinal flora in CAC may represent a new approach to improving CAC treatment.
35660350	5	8	theme	berberine	653:661	arg1	absorption					593:602	the low oral absorption	580:602	the low oral absorption into the blood	580:617	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	5	8	theme	berberine	653:661	arg1	excretion					640:648	large intestinal excretion	623:648	large intestinal excretion of berberine	623:661	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	20	9	theme	tumors	2715:2720	arg1	occurrence					2693:2702	the occurrence	2689:2702	the occurrence of colonic tumors associated with colitis in mice	2689:2752	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	6	10	theme	CAC	877:879	arg1	mice					881:884	CAC mice	877:884	CAC mice	877:884	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	22	11	theme	new	3102:3104	arg1	approach					3106:3113	a new approach	3100:3113	a new approach	3100:3113	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	17	12	theme	Berberine	2140:2148	arg1	treatment					2150:2158	Berberine treatment	2140:2158	Berberine treatment	2140:2158	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	5	13	theme	intestinal	664:673	arg1	flora					675:679	intestinal flora	664:679	intestinal flora	664:679	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	2	14	theme	intestinal	319:328	arg1	flora					330:334	intestinal flora	319:334	intestinal flora	319:334	The increasing role of intestinal flora in CAC may represent a new approach to improving CAC treatment.
35660350	12	15	theme	TLR4/NF-κB	1581:1590	arg1	P65/IL-6/p-STAT3					1592:1607	TLR4/NF-κB P65/IL-6/p-STAT3	1581:1607	TLR4/NF-κB P65/IL-6/p-STAT3	1581:1607	The signaling pathway of TLR4/NF-κB P65/IL-6/p-STAT3 was evaluated by Western blotting and immunofluorescence.
35660350	21	16	theme	Pathogenic	2892:2901	arg1	bacteria					2918:2925	(1) Pathogenic and beneficial bacteria	2888:2925	(1) Pathogenic and beneficial bacteria	2888:2925	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	6	17	from	flora	868:872	arg1	mice					881:884	CAC mice	877:884	CAC mice	877:884	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	6	17	from	flora	868:872	arg1	ability					894:900	its ability to remodel intestinal flora to improve short-chain fatty acid metabolism	890:973	its ability to remodel intestinal flora to improve short-chain fatty acid metabolism	890:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	5	18	theme	berberine	720:728	arg1	targets					709:715	the important targets	695:715	the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC)	695:781	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	6	19	theme	fatty	953:957	arg1	acid					959:962	short-chain fatty acid	941:962	short-chain fatty acid metabolism	941:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	21	20	theme	beneficial	2907:2916	arg1	bacteria					2918:2925	(1) Pathogenic and beneficial bacteria	2888:2925	(1) Pathogenic and beneficial bacteria	2888:2925	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	14	21	used	used	1828:1831	arg2	flora					1796:1800	Fecal flora transplantation (FMT)	1790:1822	Fecal flora transplantation (FMT)	1790:1822	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	7	22	theme	sodium	1059:1064	arg1	AOM/DSS					1075:1081	AOM/DSS	1075:1081	AOM/DSS	1075:1081	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	7	22	theme	sodium	1059:1064	arg1	sulfate					1066:1072	Azoxymethane/Dextran sodium sulfate	1038:1072	Azoxymethane/Dextran sodium sulfate (AOM/DSS)	1038:1082	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	9	23	theme	berberine	1208:1216	arg1	effect					1198:1203	The effect	1194:1203	The effect of berberine on colitis-associated colorectal tumorigenesis	1194:1263	The effect of berberine on colitis-associated colorectal tumorigenesis was assessed by general imaging, tumor counting, and Ki67 staining.
35660350	1	24	theme	tumor	237:241	arg1	cells					243:247	tumor cells	237:247	tumor cells	237:247	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	19	25	theme	TLR4/p-NF-κB	2481:2492	arg1	pathway					2546:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	Occludin and ZO-1 were upregulated, and the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway was inhibited with berberine.
35660350	21	26	theme	fatty	2944:2948	arg1	acids					2950:2954	Short-chain fatty acids	2932:2954	Short-chain fatty acids	2932:2954	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	9	27	theme	colitis-associated	1221:1238	arg1	tumorigenesis					1251:1263	colitis-associated colorectal tumorigenesis	1221:1263	colitis-associated colorectal tumorigenesis	1221:1263	The effect of berberine on colitis-associated colorectal tumorigenesis was assessed by general imaging, tumor counting, and Ki67 staining.
35660350	5	28	theme	low	584:586	arg1	absorption					593:602	the low oral absorption	580:602	the low oral absorption into the blood	580:617	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	1	29	theme	BACKGROUND	154:163	arg1	CAC					192:194	CAC	192:194	CAC	192:194	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	29	theme	BACKGROUND	154:163	arg1	cancer					184:189	BACKGROUND Colitis-associated cancer	154:189	BACKGROUND Colitis-associated cancer (CAC)	154:195	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	29	theme	BACKGROUND	154:163	arg1	combination					222:232	a complex combination	212:232	a complex combination of tumor cells, non-tumor cells and a large intestinal flora	212:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	22	30	from	treatment	3178:3186	arg1	practice					3207:3214	clinical practice	3198:3214	clinical practice	3198:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	18	31	theme	colitis-associated	2392:2409	arg1	tumorigenesis					2422:2434	colitis-associated colorectal tumorigenesis	2392:2434	colitis-associated colorectal tumorigenesis	2392:2434	In addition, berberine reduced LPS content in feces in mice with colitis-associated colorectal tumorigenesis.
35660350	15	32	from	mice	2002:2005	arg1	load					1980:1983	load	1980:1983	load	1980:1983	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	15	32	from	mice	2002:2005	arg1	number					1969:1974	number	1969:1974	number	1969:1974	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	5	33	theme	targets	709:715	arg1	targets					709:715	the important targets	695:715	the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC)	695:781	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	5	33	theme	targets	709:715	arg1	one					688:690	one	688:690	one	688:690	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	14	34	theme	flora	1870:1874	arg1	effect					1849:1854	the effect	1845:1854	the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine	1845:1936	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	7	35	theme	CAC	1005:1007	arg1	model					1009:1013	The CAC model	1001:1013	The CAC model	1001:1013	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	7	36	theme	STUDY	976:980	arg1	DESIGN					982:987	STUDY DESIGN	976:987	STUDY DESIGN	976:987	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	22	37	theme	berberine	3161:3169	arg1	application					3146:3156	the application	3142:3156	the application of berberine in the treatment of CAC in clinical practice	3142:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	13	38	theme	functional	1711:1720	arg1	biomarkers					1722:1731	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	38	theme	functional	1711:1720	arg1	ZO-1					1746:1749	ZO-1	1746:1749	ZO-1	1746:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	38	theme	functional	1711:1720	arg1	Occludin					1733:1740	Occludin	1733:1740	Occludin	1733:1740	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	21	39	theme	intestinal	2976:2985	arg1	flora					2987:2991	intestinal flora	2976:2991	intestinal flora	2976:2991	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	0	40	theme	colorectal	92:101	arg1	tumorigenesis					103:115	the colitis-associated colorectal tumorigenesis	69:115	the colitis-associated colorectal tumorigenesis	69:115	Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.
35660350	9	41	theme	general	1281:1287	arg1	imaging					1289:1295	general imaging	1281:1295	general imaging	1281:1295	The effect of berberine on colitis-associated colorectal tumorigenesis was assessed by general imaging, tumor counting, and Ki67 staining.
35660350	17	42	theme	acid	2231:2234	arg1	levels					2236:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	14	43	dep	flora	1796:1800	arg1	FMT					1819:1821	FMT	1819:1821	FMT	1819:1821	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	14	43	dep	flora	1796:1800	arg1	transplantation					1802:1816	transplantation	1802:1816	Fecal flora transplantation (FMT)	1790:1822	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	5	44	theme	intestinal	629:638	arg1	excretion					640:648	large intestinal excretion	623:648	large intestinal excretion of berberine	623:661	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	17	45	theme	valeric	2296:2302	arg1	acid					2304:2307	valeric acid	2296:2307	valeric acid	2296:2307	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	21	46	theme	colorectal	2832:2841	arg1	tumorigenesis					2843:2855	the colitis-associated colorectal tumorigenesis	2809:2855	the colitis-associated colorectal tumorigenesis	2809:2855	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	5	47	theme	cancer	770:775	arg1	occurrence					745:754	the occurrence	741:754	the occurrence of colorectal cancer (CRC)	741:781	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	13	48	theme	intestinal	1692:1701	arg1	biomarkers					1722:1731	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	48	theme	intestinal	1692:1701	arg1	ZO-1					1746:1749	ZO-1	1746:1749	ZO-1	1746:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	48	theme	intestinal	1692:1701	arg1	Occludin					1733:1740	Occludin	1733:1740	Occludin	1733:1740	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	11	49	theme	fatty	1428:1432	arg1	acid					1434:1437	short-chain fatty acid	1416:1437	Targeted short-chain fatty acid detection	1407:1447	Targeted short-chain fatty acid detection was performed by GC-MS/MS, and Lipopolysaccharide (LPS) levels in feces were quantified with an ELISA kit.
35660350	3	50	theme	adenoma	432:438	arg1	recurrence					440:449	colorectal adenoma recurrence	421:449	colorectal adenoma recurrence	421:449	Berberine can reduce colorectal adenoma recurrence and inhibit colorectal carcinogenesis.
35660350	1	51	theme	flora	289:293	arg1	combination					222:232	a complex combination	212:232	a complex combination of tumor cells, non-tumor cells and a large intestinal flora	212:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	51	theme	flora	289:293	arg1	cancer					184:189	BACKGROUND Colitis-associated cancer	154:189	BACKGROUND Colitis-associated cancer (CAC)	154:195	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	22	52	from	CAC	3191:3193	arg1	practice					3207:3214	clinical practice	3198:3214	clinical practice	3198:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	12	53	theme	Western	1626:1632	arg1	blotting					1634:1641	Western blotting	1626:1641	Western blotting	1626:1641	The signaling pathway of TLR4/NF-κB P65/IL-6/p-STAT3 was evaluated by Western blotting and immunofluorescence.
35660350	16	54	theme	beneficial	2062:2071	arg1	bacteria					2073:2080	pathogenic and beneficial bacteria	2047:2080	pathogenic and beneficial bacteria	2047:2080	Berberine remodeled the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis.
35660350	7	55	dep	DESIGN	982:987	arg1	model					1009:1013	The CAC model	1001:1013	The CAC model	1001:1013	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	0	56	theme	short-chain	20:30	arg1	metabolism					43:52	short-chain fatty acid metabolism	20:52	short-chain fatty acid metabolism	20:52	Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.
35660350	21	57	theme	barrier	3062:3068	arg1	function					3070:3077	intestinal barrier function	3051:3077	intestinal barrier function	3051:3077	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	17	58	theme	fecal	2185:2189	arg1	levels					2236:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	3	59	theme	colorectal	463:472	arg1	carcinogenesis					474:487	colorectal carcinogenesis	463:487	colorectal carcinogenesis	463:487	Berberine can reduce colorectal adenoma recurrence and inhibit colorectal carcinogenesis.
35660350	0	60	theme	acid	38:41	arg1	metabolism					43:52	short-chain fatty acid metabolism	20:52	short-chain fatty acid metabolism	20:52	Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.
35660350	19	61	theme	transformation	2531:2544	arg1	pathway					2546:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	Occludin and ZO-1 were upregulated, and the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway was inhibited with berberine.
35660350	15	62	dep	number	1969:1974	arg1	the					1965:1967	the	1965:1967	the	1965:1967	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	6	63	theme	intestinal	857:866	arg1	flora					868:872	intestinal flora	857:872	intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism	857:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	17	64	theme	acid	2199:2202	arg1	levels					2236:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	16	65	theme	bacteria	2073:2080	arg1	composition					2032:2042	the composition	2028:2042	the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis	2028:2137	Berberine remodeled the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis.
35660350	19	66	theme	p65/IL-6/p-STAT3	2494:2509	arg1	pathway					2546:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	Occludin and ZO-1 were upregulated, and the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway was inhibited with berberine.
35660350	6	67	theme	berberine	844:852	arg1	effects					833:839	the effects	829:839	the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism	829:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	4	68	theme	CAC	569:571	arg1	suppression					554:564	suppression	554:564	suppression	554:564	PURPOSE Berberine has demonstrated efficacy for the control and suppression of CAC.
35660350	4	68	theme	CAC	569:571	arg1	control					542:548	control	542:548	control	542:548	PURPOSE Berberine has demonstrated efficacy for the control and suppression of CAC.
35660350	16	69	theme	colorectal	2114:2123	arg1	tumorigenesis					2125:2137	colitis-associated colorectal tumorigenesis	2095:2137	colitis-associated colorectal tumorigenesis	2095:2137	Berberine remodeled the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis.
35660350	21	70	theme	transformation	3022:3035	arg1	signaling					3037:3045	(3) Inflammatory cancer transformation signaling	2998:3045	(3) Inflammatory cancer transformation signaling	2998:3045	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	17	71	theme	acetic	2205:2210	arg1	acid					2212:2215	acetic acid	2205:2215	acetic acid	2205:2215	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	20	72	theme	berberine-treated	2626:2642	arg1	sufficient					2665:2674	sufficient	2665:2674	sufficient	2665:2674	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	20	72	theme	berberine-treated	2626:2642	arg1	flora					2655:2659	the berberine-treated intestinal flora	2622:2659	the berberine-treated intestinal flora	2622:2659	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	1	73	theme	cells	243:247	arg1	combination					222:232	a complex combination	212:232	a complex combination of tumor cells, non-tumor cells and a large intestinal flora	212:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	73	theme	cells	243:247	arg1	cancer					184:189	BACKGROUND Colitis-associated cancer	154:189	BACKGROUND Colitis-associated cancer (CAC)	154:195	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	21	74	theme	Short-chain	2932:2942	arg1	acids					2950:2954	Short-chain fatty acids	2932:2954	Short-chain fatty acids	2932:2954	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	1	75	theme	non-tumor	250:258	arg1	cells					260:264	non-tumor cells	250:264	non-tumor cells	250:264	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	6	76	theme	intestinal	913:922	arg1	flora					924:928	intestinal flora	913:928	intestinal flora to improve short-chain fatty acid metabolism	913:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	4	77	dep	control	542:548	arg1	the					538:540	the	538:540	the	538:540	PURPOSE Berberine has demonstrated efficacy for the control and suppression of CAC.
35660350	10	78	theme	Intestinal	1333:1342	arg1	changes					1350:1356	Intestinal flora changes	1333:1356	Intestinal flora changes	1333:1356	Intestinal flora changes were detected by 16S rDNA sequencing technology.
35660350	2	79	theme	new	359:361	arg1	approach					363:370	a new approach	357:370	a new approach to improving CAC treatment	357:397	The increasing role of intestinal flora in CAC may represent a new approach to improving CAC treatment.
35660350	8	80	theme	50	1130:1131	arg1	doses					1121:1125	doses	1121:1125	doses of 50 and 100 mg/kg	1121:1145	Berberine was administered daily at doses of 50 and 100 mg/kg, and aspirin was used as the positive control.
35660350	10	81	theme	16S	1375:1377	arg1	sequencing					1384:1393	16S rDNA sequencing	1375:1393	16S rDNA sequencing technology	1375:1404	Intestinal flora changes were detected by 16S rDNA sequencing technology.
35660350	2	82	theme	increasing	300:309	arg1	role					311:314	The increasing role	296:314	The increasing role of intestinal flora in CAC	296:341	The increasing role of intestinal flora in CAC may represent a new approach to improving CAC treatment.
35660350	1	83	theme	large	272:276	arg1	flora					289:293	a large intestinal flora	270:293	a large intestinal flora	270:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	8	84	theme	100 mg/kg	1137:1145	arg1	doses					1121:1125	doses	1121:1125	doses of 50 and 100 mg/kg	1121:1145	Berberine was administered daily at doses of 50 and 100 mg/kg, and aspirin was used as the positive control.
35660350	22	85	theme	experimental	3119:3130	arg1	basis					3132:3136	experimental basis	3119:3136	experimental basis for the application of berberine in the treatment of CAC in clinical practice	3119:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	6	86	theme	study	804:808	arg1	purpose					788:794	The purpose	784:794	The purpose of this study	784:808	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	18	87	theme	colorectal	2411:2420	arg1	tumorigenesis					2422:2434	colitis-associated colorectal tumorigenesis	2392:2434	colitis-associated colorectal tumorigenesis	2392:2434	In addition, berberine reduced LPS content in feces in mice with colitis-associated colorectal tumorigenesis.
35660350	7	88	from	DESIGN	982:987	arg1	mice					1018:1021	mice	1018:1021	mice	1018:1021	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	2	89	theme	flora	330:334	arg1	role					311:314	The increasing role	296:314	The increasing role of intestinal flora in CAC	296:341	The increasing role of intestinal flora in CAC may represent a new approach to improving CAC treatment.
35660350	20	90	theme	colonic	2707:2713	arg1	tumors					2715:2720	colonic tumors	2707:2720	colonic tumors associated with colitis in mice	2707:2752	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	12	91	theme	P65/IL-6/p-STAT3	1592:1607	arg1	pathway					1570:1576	The signaling pathway	1556:1576	The signaling pathway of TLR4/NF-κB P65/IL-6/p-STAT3	1556:1607	The signaling pathway of TLR4/NF-κB P65/IL-6/p-STAT3 was evaluated by Western blotting and immunofluorescence.
35660350	7	92	from	METHODS	993:999	arg1	mice					1018:1021	mice	1018:1021	mice	1018:1021	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	15	93	from	number	1969:1974	arg1	mice					2002:2005	CAC mice	1998:2005	CAC mice	1998:2005	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	10	94	theme	sequencing	1384:1393	arg1	technology					1395:1404	16S rDNA sequencing technology	1375:1404	16S rDNA sequencing technology	1375:1404	Intestinal flora changes were detected by 16S rDNA sequencing technology.
35660350	1	95	theme	complex	214:220	arg1	combination					222:232	a complex combination	212:232	a complex combination of tumor cells, non-tumor cells and a large intestinal flora	212:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	95	theme	complex	214:220	arg1	cancer					184:189	BACKGROUND Colitis-associated cancer	154:189	BACKGROUND Colitis-associated cancer (CAC)	154:195	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	13	96	dep	biomarkers	1722:1731	arg1	biomarkers					1722:1731	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	96	dep	biomarkers	1722:1731	arg1	ZO-1					1746:1749	ZO-1	1746:1749	ZO-1	1746:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	96	dep	biomarkers	1722:1731	arg1	Occludin					1733:1740	Occludin	1733:1740	Occludin	1733:1740	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	6	97	theme	acid	959:962	arg1	metabolism					964:973	short-chain fatty acid metabolism	941:973	short-chain fatty acid metabolism	941:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	17	98	theme	caproic	2313:2319	arg1	acid					2321:2324	caproic acid	2313:2324	caproic acid	2313:2324	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	6	99	from	effects	833:839	arg1	flora					868:872	intestinal flora	857:872	intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism	857:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	15	100	from	load	1980:1983	arg1	mice					2002:2005	CAC mice	1998:2005	CAC mice	1998:2005	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	7	101	theme	Azoxymethane/Dextran	1038:1057	arg1	AOM/DSS					1075:1081	AOM/DSS	1075:1081	AOM/DSS	1075:1081	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	7	101	theme	Azoxymethane/Dextran	1038:1057	arg1	sulfate					1066:1072	Azoxymethane/Dextran sodium sulfate	1038:1072	Azoxymethane/Dextran sodium sulfate (AOM/DSS)	1038:1082	STUDY DESIGN AND METHODS The CAC model in mice was induced by Azoxymethane/Dextran sodium sulfate (AOM/DSS).
35660350	8	102	theme	positive	1176:1183	arg1	control					1185:1191	the positive control	1172:1191	the positive control	1172:1191	Berberine was administered daily at doses of 50 and 100 mg/kg, and aspirin was used as the positive control.
35660350	8	102	theme	positive	1176:1183	arg1	aspirin					1152:1158	aspirin	1152:1158	aspirin	1152:1158	Berberine was administered daily at doses of 50 and 100 mg/kg, and aspirin was used as the positive control.
35660350	6	103	theme	short-chain	941:951	arg1	acid					959:962	short-chain fatty acid	941:962	short-chain fatty acid metabolism	941:973	The purpose of this study was to investigate the effects of berberine on intestinal flora in CAC mice and its ability to remodel intestinal flora to improve short-chain fatty acid metabolism.
35660350	8	104	used	used	1164:1167	arg2	aspirin					1152:1158	aspirin	1152:1158	aspirin	1152:1158	Berberine was administered daily at doses of 50 and 100 mg/kg, and aspirin was used as the positive control.
35660350	8	104	used	used	1164:1167	arg2	control					1185:1191	the positive control	1172:1191	the positive control	1172:1191	Berberine was administered daily at doses of 50 and 100 mg/kg, and aspirin was used as the positive control.
35660350	4	105	theme	PURPOSE	490:496	arg1	Berberine					498:506	PURPOSE Berberine	490:506	PURPOSE Berberine	490:506	PURPOSE Berberine has demonstrated efficacy for the control and suppression of CAC.
35660350	15	106	from	tumors	1988:1993	arg1	mice					2002:2005	CAC mice	1998:2005	CAC mice	1998:2005	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	14	107	theme	inflammatory	1890:1901	arg1	transformation					1910:1923	inflammatory cancer transformation	1890:1923	inflammatory cancer transformation	1890:1923	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	18	108	theme	LPS	2358:2360	arg1	content					2362:2368	LPS content	2358:2368	LPS content in feces	2358:2377	In addition, berberine reduced LPS content in feces in mice with colitis-associated colorectal tumorigenesis.
35660350	11	109	theme	Lipopolysaccharide	1480:1497	arg1	levels					1505:1510	Lipopolysaccharide (LPS) levels	1480:1510	Lipopolysaccharide (LPS) levels in feces	1480:1519	Targeted short-chain fatty acid detection was performed by GC-MS/MS, and Lipopolysaccharide (LPS) levels in feces were quantified with an ELISA kit.
35660350	11	110	theme	ELISA	1545:1549	arg1	kit					1551:1553	an ELISA kit	1542:1553	an ELISA kit	1542:1553	Targeted short-chain fatty acid detection was performed by GC-MS/MS, and Lipopolysaccharide (LPS) levels in feces were quantified with an ELISA kit.
35660350	5	111	theme	important	699:707	arg1	targets					709:715	the important targets	695:715	the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC)	695:781	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	9	112	theme	colorectal	1240:1249	arg1	tumorigenesis					1251:1263	colitis-associated colorectal tumorigenesis	1221:1263	colitis-associated colorectal tumorigenesis	1221:1263	The effect of berberine on colitis-associated colorectal tumorigenesis was assessed by general imaging, tumor counting, and Ki67 staining.
35660350	21	113	dep	CONCLUSION	2755:2764	arg1	showed					2776:2781	showed	2776:2781	showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function	2776:3077	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	5	114	theme	oral	588:591	arg1	absorption					593:602	the low oral absorption	580:602	the low oral absorption into the blood	580:617	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	1	115	theme	Colitis-associated	165:182	arg1	CAC					192:194	CAC	192:194	CAC	192:194	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	115	theme	Colitis-associated	165:182	arg1	cancer					184:189	BACKGROUND Colitis-associated cancer	154:189	BACKGROUND Colitis-associated cancer (CAC)	154:195	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	115	theme	Colitis-associated	165:182	arg1	combination					222:232	a complex combination	212:232	a complex combination of tumor cells, non-tumor cells and a large intestinal flora	212:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	0	116	theme	colitis-associated	73:90	arg1	tumorigenesis					103:115	the colitis-associated colorectal tumorigenesis	69:115	the colitis-associated colorectal tumorigenesis	69:115	Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.
35660350	14	117	theme	intestinal	1859:1868	arg1	flora					1870:1874	intestinal flora	1859:1874	intestinal flora	1859:1874	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	15	118	theme	RESULTS	1939:1945	arg1	Berberine					1947:1955	RESULTS Berberine	1939:1955	RESULTS Berberine	1939:1955	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	21	119	theme	Inflammatory	3002:3013	arg1	signaling					3037:3045	(3) Inflammatory cancer transformation signaling	2998:3045	(3) Inflammatory cancer transformation signaling	2998:3045	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	2	120	from	role	311:314	arg1	CAC					339:341	CAC	339:341	CAC	339:341	The increasing role of intestinal flora in CAC may represent a new approach to improving CAC treatment.
35660350	17	121	theme	isovaleric	2279:2288	arg1	acid					2290:2293	isovaleric acid	2279:2293	isovaleric acid	2279:2293	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	13	122	theme	biomarkers	1722:1731	arg1	levels					1682:1687	The expression levels	1667:1687	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1	1667:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	16	123	from	composition	2032:2042	arg1	mice					2085:2088	mice	2085:2088	mice with colitis-associated colorectal tumorigenesis	2085:2137	Berberine remodeled the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis.
35660350	22	124	from	practice	3207:3214	arg1	treatment					3178:3186	the treatment	3174:3186	the treatment of CAC in clinical practice	3174:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	0	125	theme	remodeling	125:134	arg1	flora					147:151	remodeling intestinal flora	125:151	remodeling intestinal flora	125:151	Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.
35660350	20	126	from	colitis	2738:2744	arg1	mice					2749:2752	mice	2749:2752	mice	2749:2752	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	5	127	theme	large	623:627	arg1	excretion					640:648	large intestinal excretion	623:648	large intestinal excretion of berberine	623:661	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	16	128	with	mice	2085:2088	arg1	tumorigenesis					2125:2137	colitis-associated colorectal tumorigenesis	2095:2137	colitis-associated colorectal tumorigenesis	2095:2137	Berberine remodeled the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis.
35660350	15	129	theme	tumors	1988:1993	arg1	load					1980:1983	load	1980:1983	load	1980:1983	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	15	129	theme	tumors	1988:1993	arg1	number					1969:1974	number	1969:1974	number	1969:1974	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	21	130	theme	colitis-associated	2813:2830	arg1	tumorigenesis					2843:2855	the colitis-associated colorectal tumorigenesis	2809:2855	the colitis-associated colorectal tumorigenesis	2809:2855	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	11	131	theme	acid	1434:1437	arg1	detection					1439:1447	Targeted short-chain fatty acid detection	1407:1447	Targeted short-chain fatty acid detection	1407:1447	Targeted short-chain fatty acid detection was performed by GC-MS/MS, and Lipopolysaccharide (LPS) levels in feces were quantified with an ELISA kit.
35660350	5	132	theme	colorectal	759:768	arg1	CRC					778:780	CRC	778:780	CRC	778:780	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	5	132	theme	colorectal	759:768	arg1	cancer					770:775	colorectal cancer	759:775	colorectal cancer (CRC)	759:781	Given the low oral absorption into the blood and large intestinal excretion of berberine, intestinal flora may be one of the important targets of berberine inhibiting the occurrence of colorectal cancer (CRC).
35660350	9	133	theme	tumor	1298:1302	arg1	counting					1304:1311	tumor counting	1298:1311	tumor counting	1298:1311	The effect of berberine on colitis-associated colorectal tumorigenesis was assessed by general imaging, tumor counting, and Ki67 staining.
35660350	20	134	theme	FMT	2588:2590	arg1	results					2592:2598	The FMT results	2584:2598	The FMT results	2584:2598	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	11	135	from	levels	1505:1510	arg1	feces					1515:1519	feces	1515:1519	feces	1515:1519	Targeted short-chain fatty acid detection was performed by GC-MS/MS, and Lipopolysaccharide (LPS) levels in feces were quantified with an ELISA kit.
35660350	22	136	from	application	3146:3156	arg1	treatment					3178:3186	the treatment	3174:3186	the treatment of CAC in clinical practice	3174:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	11	137	theme	short-chain	1416:1426	arg1	acid					1434:1437	short-chain fatty acid	1416:1437	Targeted short-chain fatty acid detection	1407:1447	Targeted short-chain fatty acid detection was performed by GC-MS/MS, and Lipopolysaccharide (LPS) levels in feces were quantified with an ELISA kit.
35660350	13	138	theme	barrier	1703:1709	arg1	biomarkers					1722:1731	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	intestinal barrier functional biomarkers Occludin and ZO-1	1692:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	138	theme	barrier	1703:1709	arg1	ZO-1					1746:1749	ZO-1	1746:1749	ZO-1	1746:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	13	138	theme	barrier	1703:1709	arg1	Occludin					1733:1740	Occludin	1733:1740	Occludin	1733:1740	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	0	139	theme	fatty	32:36	arg1	metabolism					43:52	short-chain fatty acid metabolism	20:52	short-chain fatty acid metabolism	20:52	Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.
35660350	9	140	theme	Ki67	1318:1321	arg1	staining					1323:1330	Ki67 staining	1318:1330	Ki67 staining	1318:1330	The effect of berberine on colitis-associated colorectal tumorigenesis was assessed by general imaging, tumor counting, and Ki67 staining.
35660350	16	141	theme	pathogenic	2047:2056	arg1	bacteria					2073:2080	pathogenic and beneficial bacteria	2047:2080	pathogenic and beneficial bacteria	2047:2080	Berberine remodeled the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis.
35660350	22	142	theme	clinical	3198:3205	arg1	practice					3207:3214	clinical practice	3198:3214	clinical practice	3198:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	21	143	theme	intestinal	3051:3060	arg1	function					3070:3077	intestinal barrier function	3051:3077	intestinal barrier function	3051:3077	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	17	144	theme	isobutyric	2262:2271	arg1	acid					2273:2276	isobutyric acid	2262:2276	isobutyric acid	2262:2276	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	3	145	theme	colorectal	421:430	arg1	recurrence					440:449	colorectal adenoma recurrence	421:449	colorectal adenoma recurrence	421:449	Berberine can reduce colorectal adenoma recurrence and inhibit colorectal carcinogenesis.
35660350	18	146	with	mice	2382:2385	arg1	tumorigenesis					2422:2434	colitis-associated colorectal tumorigenesis	2392:2434	colitis-associated colorectal tumorigenesis	2392:2434	In addition, berberine reduced LPS content in feces in mice with colitis-associated colorectal tumorigenesis.
35660350	13	147	theme	expression	1671:1680	arg1	levels					1682:1687	The expression levels	1667:1687	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1	1667:1749	The expression levels of intestinal barrier functional biomarkers Occludin and ZO-1 were detected by immunohistochemistry.
35660350	17	148	theme	butyric	2191:2197	arg1	acid					2199:2202	butyric acid	2191:2202	butyric acid	2191:2202	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	17	149	from	increases	2172:2180	arg1	levels					2236:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	0	150	theme	intestinal	136:145	arg1	flora					147:151	remodeling intestinal flora	125:151	remodeling intestinal flora	125:151	Berberine regulates short-chain fatty acid metabolism and alleviates the colitis-associated colorectal tumorigenesis through remodeling intestinal flora.
35660350	12	151	theme	signaling	1560:1568	arg1	pathway					1570:1576	The signaling pathway	1556:1576	The signaling pathway of TLR4/NF-κB P65/IL-6/p-STAT3	1556:1607	The signaling pathway of TLR4/NF-κB P65/IL-6/p-STAT3 was evaluated by Western blotting and immunofluorescence.
35660350	22	152	theme	CAC	3191:3193	arg1	treatment					3178:3186	the treatment	3174:3186	the treatment of CAC in clinical practice	3174:3214	This study provided a new approach and experimental basis for the application of berberine in the treatment of CAC in clinical practice.
35660350	21	153	theme	cancer	3015:3020	arg1	signaling					3037:3045	(3) Inflammatory cancer transformation signaling	2998:3045	(3) Inflammatory cancer transformation signaling	2998:3045	CONCLUSION Our study showed that berberine alleviated the colitis-associated colorectal tumorigenesis from three equilibrium levels: (1) Pathogenic and beneficial bacteria; (2) Short-chain fatty acids and LPS produced by intestinal flora; and (3) Inflammatory cancer transformation signaling and intestinal barrier function.
35660350	15	154	theme	CAC	1998:2000	arg1	mice					2002:2005	CAC mice	1998:2005	CAC mice	1998:2005	RESULTS Berberine reduced the number and load of tumors in CAC mice.
35660350	20	155	theme	intestinal	2644:2653	arg1	sufficient					2665:2674	sufficient	2665:2674	sufficient	2665:2674	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	20	155	theme	intestinal	2644:2653	arg1	flora					2655:2659	the berberine-treated intestinal flora	2622:2659	the berberine-treated intestinal flora	2622:2659	The FMT results further verified that the berberine-treated intestinal flora was sufficient to alleviate the occurrence of colonic tumors associated with colitis in mice.
35660350	19	156	theme	inflammatory-cancer	2511:2529	arg1	pathway					2546:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway	2477:2552	Occludin and ZO-1 were upregulated, and the TLR4/p-NF-κB p65/IL-6/p-STAT3 inflammatory-cancer transformation pathway was inhibited with berberine.
35660350	16	157	theme	colitis-associated	2095:2112	arg1	tumorigenesis					2125:2137	colitis-associated colorectal tumorigenesis	2095:2137	colitis-associated colorectal tumorigenesis	2095:2137	Berberine remodeled the composition of pathogenic and beneficial bacteria in mice with colitis-associated colorectal tumorigenesis.
35660350	17	158	theme	acid	2212:2215	arg1	levels					2236:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	fecal butyric acid, acetic acid and propionic acid levels	2185:2241	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	14	159	theme	Fecal	1790:1794	arg1	flora					1796:1800	Fecal flora transplantation (FMT)	1790:1822	Fecal flora transplantation (FMT)	1790:1822	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	14	160	theme	cancer	1903:1908	arg1	transformation					1910:1923	inflammatory cancer transformation	1890:1923	inflammatory cancer transformation	1890:1923	Fecal flora transplantation (FMT) was used to evaluate the effect of intestinal flora in inhibiting inflammatory cancer transformation by berberine.
35660350	17	161	theme	propionic	2221:2229	arg1	acid					2231:2234	propionic acid	2221:2234	propionic acid	2221:2234	Berberine treatment resulted in increases in fecal butyric acid, acetic acid and propionic acid levels, but did not alter isobutyric acid, isovaleric acid, valeric acid and caproic acid.
35660350	10	162	theme	flora	1344:1348	arg1	changes					1350:1356	Intestinal flora changes	1333:1356	Intestinal flora changes	1333:1356	Intestinal flora changes were detected by 16S rDNA sequencing technology.
35660350	1	163	theme	cells	260:264	arg1	combination					222:232	a complex combination	212:232	a complex combination of tumor cells, non-tumor cells and a large intestinal flora	212:293	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35660350	1	163	theme	cells	260:264	arg1	cancer					184:189	BACKGROUND Colitis-associated cancer	154:189	BACKGROUND Colitis-associated cancer (CAC)	154:195	BACKGROUND Colitis-associated cancer (CAC) is known to be a complex combination of tumor cells, non-tumor cells and a large intestinal flora.
35984616	7	0	theme	strain	1488:1493	arg1	HY170T					1495:1500	strain HY170T	1488:1500	strain HY170T	1488:1500	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	5	1	theme	predominant	1132:1142	arg1	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol (DPG)	1044:1071	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	1	theme	predominant	1132:1142	arg1	MK-8					1034:1037	MK-8	1034:1037	MK-8(H2)	1034:1041	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	1	theme	predominant	1132:1142	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	1	theme	predominant	1132:1142	arg1	menaquinone					1144:1154	the predominant menaquinone	1128:1154	the predominant menaquinone	1128:1154	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	1	theme	predominant	1132:1142	arg1	phosphatidylglycerol					1077:1096	phosphatidylglycerol	1077:1096	phosphatidylglycerol (PG)	1077:1101	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	1	theme	predominant	1132:1142	arg1	ribose					1118:1123	ribose	1118:1123	ribose	1118:1123	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	7	2	theme	predominant	1473:1483	arg1	anteiso-C17:1A					1458:1471	anteiso-C17:1A	1458:1471	anteiso-C17:1A predominant in strain HY170T but absent in strain HY001	1458:1527	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	4	3	theme	distinct	841:848	arg1	lineage					850:856	a distinct lineage	839:856	a distinct lineage	839:856	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	10	4	theme	=	1956:1956	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	2	5	dep	bacteria	250:257	arg1	bacteria					250:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria	137:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001)	137:276	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	5	dep	bacteria	250:257	arg1	HY001					271:275	HY001	271:275	HY001	271:275	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	5	dep	bacteria	250:257	arg1	HY170T					260:265	HY170T	260:265	HY170T	260:265	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	3	6	theme	blood	586:590	arg1	plate					592:596	BHI-5% sheep blood plate	573:596	BHI-5% sheep blood plate with pH 7.5	573:608	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	9	7	theme	Brevibacterium	1854:1867	arg1	species					1833:1839	a novel species	1825:1839	a novel species	1825:1839	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	9	7	theme	Brevibacterium	1854:1867	arg1	strains					1794:1800	the two strains	1786:1800	the two strains	1786:1800	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	10	8	theme	strain	1942:1947	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	4	9	theme	aurantiacum	918:928	arg1	739T					935:938	B. aurantiacum NCDO 739T	915:938	B. aurantiacum NCDO 739T	915:938	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	2	10	theme	bat	297:299	arg1	feces					301:305	bat feces	297:305	bat feces collected from Chongzuo city, Guangxi province	297:352	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	4	11	theme	phylogenomic	715:726	arg1	trees					728:732	The phylogenetic and phylogenomic trees	694:732	trees	728:732	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	8	12	theme	secondary	1574:1582	arg1	clusters					1613:1620	secondary metabolite biosynthesis gene clusters	1574:1620	secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene	1574:1703	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	7	13	from	predominant	1473:1483	arg1	HY170T					1495:1500	strain HY170T	1488:1500	strain HY170T	1488:1500	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	5	14	from	lipids	1169:1174	arg1	wall					1205:1208	the cell wall teichoic acids	1196:1223	the cell wall teichoic acids	1196:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	10	15	theme	1.18600T	1964:1971	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	8	16	theme	biosynthesis	1595:1606	arg1	clusters					1613:1620	secondary metabolite biosynthesis gene clusters	1574:1620	secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene	1574:1703	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	2	17	theme	1.9	242:244	arg1	×					240:240	×	240:240	×	240:240	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	6	18	theme	diagnostic	1288:1297	arg1	acid					1307:1310	the diagnostic diamino acid	1284:1310	the diagnostic diamino acid of the peptidoglycan found in strain HY170T	1284:1354	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	6	18	theme	diagnostic	1288:1297	arg1	acid					1264:1267	The meso-diaminopimelic acid	1240:1267	The meso-diaminopimelic acid (meso-DAP)	1240:1278	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	5	19	from	sugars	1186:1191	arg1	wall					1205:1208	the cell wall teichoic acids	1196:1223	the cell wall teichoic acids	1196:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	3	20	theme	5.0-10.0	618:625	arg1	range					611:615	range	611:615	range	611:615	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	8	21	theme	clusters	1613:1620	arg1	presence					1562:1569	the presence	1558:1569	the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene	1558:1703	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	3	22	dep	NaCl	656:659	arg1	[w/v					686:689	0-15% NaCl [w/v	675:689	0-15% NaCl [w/v	675:689	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	3	22	dep	NaCl	656:659	arg1	range					668:672	range	668:672	range	668:672	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	2	23	theme	0.7	236:238	arg1	×					240:240	×	240:240	×	240:240	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	1	24	theme	bat	126:128	arg1	feces					130:134	bat feces	126:134	bat feces	126:134	nov., a novel halotolerant actinomycete isolated from bat feces.
35984616	6	25	theme	diamino	1299:1305	arg1	acid					1307:1310	the diagnostic diamino acid	1284:1310	the diagnostic diamino acid of the peptidoglycan found in strain HY170T	1284:1354	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	6	25	theme	diamino	1299:1305	arg1	acid					1264:1267	The meso-diaminopimelic acid	1240:1267	The meso-diaminopimelic acid (meso-DAP)	1240:1278	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	4	26	theme	phylogenetic	698:709	arg1	trees					728:732	The phylogenetic and phylogenomic trees	694:732	trees	728:732	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	5	27	theme	Strain	1010:1015	arg1	HY170T					1017:1022	Strain HY170T	1010:1022	Strain HY170T	1010:1022	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	3	28	theme	0.5-1.0	647:653	arg1	%					654:654	%	654:654	%	654:654	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	8	29	theme	poly-amino	1645:1654	arg1	terpene					1697:1703	terpene	1697:1703	terpene	1697:1703	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	29	theme	poly-amino	1645:1654	arg1	siderophore					1680:1690	siderophore	1680:1690	siderophore	1680:1690	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	29	theme	poly-amino	1645:1654	arg1	NAPAA					1663:1667	NAPAA	1663:1667	NAPAA	1663:1667	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	29	theme	poly-amino	1645:1654	arg1	ectoine					1671:1677	ectoine	1671:1677	ectoine	1671:1677	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	29	theme	poly-amino	1645:1654	arg1	acids					1656:1660	non-alpha poly-amino acids	1635:1660	non-alpha poly-amino acids (NAPAA)	1635:1668	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	3	30	theme	Optimal	516:522	arg1	growth					524:529	Optimal growth	516:529	Optimal growth	516:529	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	7	31	with	acids	1410:1414	arg1	anteiso-C17:1A					1458:1471	anteiso-C17:1A	1458:1471	anteiso-C17:1A predominant in strain HY170T but absent in strain HY001	1458:1527	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	5	32	theme	main	1181:1184	arg1	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol (DPG)	1044:1071	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	32	theme	main	1181:1184	arg1	MK-8					1034:1037	MK-8	1034:1037	MK-8(H2)	1034:1041	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	32	theme	main	1181:1184	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	32	theme	main	1181:1184	arg1	phosphatidylglycerol					1077:1096	phosphatidylglycerol	1077:1096	phosphatidylglycerol (PG)	1077:1101	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	32	theme	main	1181:1184	arg1	sugars					1186:1191	main sugars	1181:1191	main sugars in the cell wall teichoic acids	1181:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	32	theme	main	1181:1184	arg1	ribose					1118:1123	ribose	1118:1123	ribose	1118:1123	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	4	33	theme	16S	941:943	arg1	similarity					950:959	16S rRNA similarity	941:959	16S rRNA similarity	941:959	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	9	34	theme	Brevibacterium	1889:1902	arg1	sp					1915:1916	the name Brevibacterium zhoupengii sp	1880:1916	the name Brevibacterium zhoupengii sp	1880:1916	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	6	35	theme	strain	1342:1347	arg1	HY170T					1349:1354	strain HY170T	1342:1354	strain HY170T	1342:1354	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	3	36	theme	%	679:679	arg1	[w/v					686:689	0-15% NaCl [w/v	675:689	0-15% NaCl [w/v	675:689	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	3	36	theme	%	679:679	arg1	range					668:672	range	668:672	range	668:672	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	3	37	theme	NaCl	656:659	arg1	presence					635:642	the presence	631:642	the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v])	631:691	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	4	38	theme	16S	760:762	arg1	gene					769:772	the 16S rRNA gene and 845 core gene sequences	756:800	gene	769:772	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	5	39	theme	polar	1163:1167	arg1	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol (DPG)	1044:1071	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	39	theme	polar	1163:1167	arg1	lipids					1169:1174	major polar lipids	1157:1174	major polar lipids	1157:1174	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	39	theme	polar	1163:1167	arg1	MK-8					1034:1037	MK-8	1034:1037	MK-8(H2)	1034:1041	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	39	theme	polar	1163:1167	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	39	theme	polar	1163:1167	arg1	phosphatidylglycerol					1077:1096	phosphatidylglycerol	1077:1096	phosphatidylglycerol (PG)	1077:1101	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	39	theme	polar	1163:1167	arg1	ribose					1118:1123	ribose	1118:1123	ribose	1118:1123	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	0	40	theme	Phenotypic	0:9	arg1	characteristics					23:37	Phenotypic and genomic characteristics	0:37	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.	0:70	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.
35984616	1	41	theme	halotolerant	86:97	arg1	actinomycete					99:110	a novel halotolerant actinomycete	78:110	a novel halotolerant actinomycete	78:110	nov., a novel halotolerant actinomycete isolated from bat feces.
35984616	1	41	theme	halotolerant	86:97	arg1	nov.					72:75	nov.	72:75	nov.	72:75	nov., a novel halotolerant actinomycete isolated from bat feces.
35984616	0	42	theme	genomic	15:21	arg1	characteristics					23:37	Phenotypic and genomic characteristics	0:37	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.	0:70	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.
35984616	4	43	theme	gene	787:790	arg1	sequences					792:800	the 16S rRNA gene and 845 core gene sequences	756:800	sequences	792:800	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	2	44	dep	aerobic	150:156	arg1	non-spore-forming					183:199	non-spore-forming	183:199	non-spore-forming	183:199	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	44	dep	aerobic	150:156	arg1	Gram-staining-positive					159:180	Gram-staining-positive	159:180	Gram-staining-positive	159:180	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	44	dep	aerobic	150:156	arg1	regular					202:208	regular	202:208	regular	202:208	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	5	45	dep	wall	1205:1208	arg1	acids					1219:1223	teichoic acids	1210:1223	the cell wall teichoic acids	1196:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	46	theme	cell	1200:1203	arg1	wall					1205:1208	the cell wall teichoic acids	1196:1223	the cell wall teichoic acids	1196:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	9	47	from	analyses	1776:1783	arg1	results					1715:1721	results	1715:1721	results from the phylogenetic, chemotaxonomic and phenotypic analyses	1715:1783	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	0	48	theme	zhoupengii	57:66	arg1	sp					68:69	Brevibacterium zhoupengii sp	42:69	Brevibacterium zhoupengii sp	42:69	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.
35984616	7	49	theme	absent	1506:1511	arg1	anteiso-C17:1A					1458:1471	anteiso-C17:1A	1458:1471	anteiso-C17:1A predominant in strain HY170T but absent in strain HY001	1458:1527	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	5	50	theme	teichoic	1210:1217	arg1	acids					1219:1223	teichoic acids	1210:1223	the cell wall teichoic acids	1196:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	3	51	with	plate	592:596	arg1	pH					603:604	pH 7.5	603:608	pH 7.5	603:608	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	2	52	dep	province	437:444	arg1	39″E					466:469	39″E	466:469	39″E	466:469	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	52	dep	province	437:444	arg1	2013					480:483	October 2013	472:483	October 2013	472:483	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	52	dep	province	437:444	arg1	10″N					453:456	10″N	453:456	10″N	453:456	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	3	53	dep	25-28°C	546:552	arg1	range					611:615	range	611:615	range	611:615	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	3	53	dep	25-28°C	546:552	arg1	4-32°C					562:567	4-32°C	562:567	4-32°C	562:567	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	3	53	dep	25-28°C	546:552	arg1	range					555:559	range	555:559	range	555:559	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	7	54	dep	strains	1427:1433	arg1	strains					1427:1433	strains HY170T and HY001	1427:1450	strains HY170T and HY001	1427:1450	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	54	dep	strains	1427:1433	arg1	HY001					1446:1450	HY001	1446:1450	HY001	1446:1450	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	54	dep	strains	1427:1433	arg1	HY170T					1435:1440	HY170T	1435:1440	HY170T	1435:1440	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	3	55	theme	sheep	580:584	arg1	plate					592:596	BHI-5% sheep blood plate	573:596	BHI-5% sheep blood plate with pH 7.5	573:608	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	5	56	from	menaquinone	1144:1154	arg1	wall					1205:1208	the cell wall teichoic acids	1196:1223	the cell wall teichoic acids	1196:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	3	57	theme	BHI-5	573:577	arg1	%					578:578	%	578:578	%	578:578	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	4	58	theme	B.	915:916	arg1	739T					935:938	B. aurantiacum NCDO 739T	915:938	B. aurantiacum NCDO 739T	915:938	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	3	59	from	25-28°C	546:552	arg1	plate					592:596	BHI-5% sheep blood plate	573:596	BHI-5% sheep blood plate with pH 7.5	573:608	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	2	60	theme	aerobic	150:156	arg1	bacteria					250:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria	137:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001)	137:276	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	60	theme	aerobic	150:156	arg1	mm					246:247	approximately 0.7 × 1.9 mm	222:247	approximately 0.7 × 1.9 mm	222:247	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	60	theme	aerobic	150:156	arg1	HY001					271:275	HY001	271:275	HY001	271:275	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	60	theme	aerobic	150:156	arg1	HY170T					260:265	HY170T	260:265	HY170T	260:265	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	5	61	theme	major	1157:1161	arg1	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol (DPG)	1044:1071	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	61	theme	major	1157:1161	arg1	lipids					1169:1174	major polar lipids	1157:1174	major polar lipids	1157:1174	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	61	theme	major	1157:1161	arg1	MK-8					1034:1037	MK-8	1034:1037	MK-8(H2)	1034:1041	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	61	theme	major	1157:1161	arg1	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	61	theme	major	1157:1161	arg1	phosphatidylglycerol					1077:1096	phosphatidylglycerol	1077:1096	phosphatidylglycerol (PG)	1077:1101	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	61	theme	major	1157:1161	arg1	ribose					1118:1123	ribose	1118:1123	ribose	1118:1123	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	6	62	located	found	1333:1337	arg1	HY170T					1349:1354	strain HY170T	1342:1354	strain HY170T	1342:1354	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	6	62	located	found	1333:1337	arg2	peptidoglycan					1319:1331	the peptidoglycan	1315:1331	the peptidoglycan found in strain HY170T	1315:1354	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	10	63	theme	HY170T	1949:1954	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	4	64	theme	NCDO	930:933	arg1	739T					935:938	B. aurantiacum NCDO 739T	915:938	B. aurantiacum NCDO 739T	915:938	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	10	65	theme	type	1937:1940	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	7	66	theme	strains	1427:1433	arg1	Anteiso-C15:0					1357:1369	Anteiso-C15:0	1357:1369	Anteiso-C15:0	1357:1369	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	66	theme	strains	1427:1433	arg1	%					1421:1421	> 10%	1417:1421	> 10%	1417:1421	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	66	theme	strains	1427:1433	arg1	acids					1410:1414	the major fatty acids	1394:1414	the major fatty acids (> 10%) of strains HY170T and HY001	1394:1450	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	66	theme	strains	1427:1433	arg1	anteiso-C17:0					1375:1387	anteiso-C17:0	1375:1387	anteiso-C17:0	1375:1387	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	9	67	theme	novel	1827:1831	arg1	species					1833:1839	a novel species	1825:1839	a novel species	1825:1839	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	9	67	theme	novel	1827:1831	arg1	strains					1794:1800	the two strains	1786:1800	the two strains	1786:1800	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	8	68	theme	metabolite	1584:1593	arg1	clusters					1613:1620	secondary metabolite biosynthesis gene clusters	1574:1620	secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene	1574:1703	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	2	69	attach	isolated	283:290	arg2	HY170T					260:265	HY170T	260:265	HY170T	260:265	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	69	attach	isolated	283:290	arg2	bacteria					250:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria	137:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001)	137:276	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	69	attach	isolated	283:290	arg2	mm					246:247	approximately 0.7 × 1.9 mm	222:247	approximately 0.7 × 1.9 mm	222:247	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	69	attach	isolated	283:290	arg2	HY001					271:275	HY001	271:275	HY001	271:275	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	69	attach	isolated	283:290	arg1	feces					301:305	bat feces	297:305	bat feces collected from Chongzuo city, Guangxi province	297:352	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	10	70	theme	=	1973:1973	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	4	71	theme	related	904:910	arg1	Brevibacterium					875:888	the genus Brevibacterium	865:888	the genus Brevibacterium	865:888	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	7	72	attach	predominant	1473:1483	arg1	HY170T					1495:1500	strain HY170T	1488:1500	strain HY170T	1488:1500	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	72	attach	predominant	1473:1483	arg2	anteiso-C17:1A					1458:1471	anteiso-C17:1A	1458:1471	anteiso-C17:1A predominant in strain HY170T but absent in strain HY001	1458:1527	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	8	73	theme	gene	1608:1611	arg1	clusters					1613:1620	secondary metabolite biosynthesis gene clusters	1574:1620	secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene	1574:1703	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	7	74	theme	fatty	1404:1408	arg1	Anteiso-C15:0					1357:1369	Anteiso-C15:0	1357:1369	Anteiso-C15:0	1357:1369	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	74	theme	fatty	1404:1408	arg1	%					1421:1421	> 10%	1417:1421	> 10%	1417:1421	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	74	theme	fatty	1404:1408	arg1	acids					1410:1414	the major fatty acids	1394:1414	the major fatty acids (> 10%) of strains HY170T and HY001	1394:1450	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	74	theme	fatty	1404:1408	arg1	anteiso-C17:0					1375:1387	anteiso-C17:0	1375:1387	anteiso-C17:0	1375:1387	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	5	75	contain	contained	1024:1032	arg2	MK-8					1034:1037	MK-8	1034:1037	MK-8(H2)	1034:1041	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	sugars					1186:1191	main sugars	1181:1191	main sugars in the cell wall teichoic acids	1181:1223	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	H2					1039:1040	H2	1039:1040	H2	1039:1040	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	diphosphatidylglycerol					1044:1065	diphosphatidylglycerol	1044:1065	diphosphatidylglycerol (DPG)	1044:1071	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	ribose					1118:1123	ribose	1118:1123	ribose	1118:1123	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	phosphatidylglycerol					1077:1096	phosphatidylglycerol	1077:1096	phosphatidylglycerol (PG)	1077:1101	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	DPG					1068:1070	DPG	1068:1070	DPG	1068:1070	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	PG					1099:1100	PG	1099:1100	PG	1099:1100	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	lipids					1169:1174	major polar lipids	1157:1174	major polar lipids	1157:1174	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	galactose					1104:1112	galactose	1104:1112	galactose	1104:1112	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg2	menaquinone					1144:1154	the predominant menaquinone	1128:1154	the predominant menaquinone	1128:1154	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	5	75	contain	contained	1024:1032	arg1	HY170T					1017:1022	Strain HY170T	1010:1022	Strain HY170T	1010:1022	Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively.
35984616	4	76	dep	similarity	950:959	arg1	dDDH					974:977	dDDH	974:977	dDDH	974:977	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	4	76	dep	similarity	950:959	arg1	%					989:989	46.7-46.8%	980:989	46.7-46.8%	980:989	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	4	76	dep	similarity	950:959	arg1	%					1006:1006	91.9-92.1%	997:1006	91.9-92.1%	997:1006	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	4	76	dep	similarity	950:959	arg1	both					962:965	both	962:965	both	962:965	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	4	76	dep	similarity	950:959	arg1	ANI					992:994	ANI	992:994	ANI	992:994	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	10	77	theme	CGMCC	1958:1962	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	9	78	theme	genus	1848:1852	arg1	Brevibacterium					1854:1867	the genus Brevibacterium	1844:1867	the genus Brevibacterium	1844:1867	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	10	79	dep	proposed	1927:1934	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	10	80	theme	JCM	1975:1977	arg1	34230T					1979:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	type strain HY170T = CGMCC 1.18600T = JCM 34230T	1937:1984	nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).
35984616	2	81	dep	isolated	283:290	arg1	54″N					361:364	22°20'54″N	355:364	22°20'54″N	355:364	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	8	82	theme	non-alpha	1635:1643	arg1	terpene					1697:1703	terpene	1697:1703	terpene	1697:1703	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	82	theme	non-alpha	1635:1643	arg1	siderophore					1680:1690	siderophore	1680:1690	siderophore	1680:1690	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	82	theme	non-alpha	1635:1643	arg1	NAPAA					1663:1667	NAPAA	1663:1667	NAPAA	1663:1667	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	82	theme	non-alpha	1635:1643	arg1	ectoine					1671:1677	ectoine	1671:1677	ectoine	1671:1677	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	8	82	theme	non-alpha	1635:1643	arg1	acids					1656:1660	non-alpha poly-amino acids	1635:1660	non-alpha poly-amino acids (NAPAA)	1635:1668	Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene.
35984616	3	83	theme	0-15	675:678	arg1	%					679:679	%	679:679	%	679:679	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	2	84	theme	October	472:478	arg1	2013					480:483	October 2013	472:483	October 2013	472:483	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	84	theme	October	472:478	arg1	10″N					453:456	10″N	453:456	10″N	453:456	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	3	85	theme	%	654:654	arg1	w/v					662:664	w/v	662:664	w/v	662:664	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	3	85	theme	%	654:654	arg1	NaCl					656:659	0.5-1.0% NaCl	647:659	0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v])	647:691	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	4	86	theme	rRNA	764:767	arg1	gene					769:772	the 16S rRNA gene and 845 core gene sequences	756:800	gene	769:772	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	4	87	theme	rRNA	945:948	arg1	similarity					950:959	16S rRNA similarity	941:959	16S rRNA similarity	941:959	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	9	88	theme	name	1884:1887	arg1	sp					1915:1916	the name Brevibacterium zhoupengii sp	1880:1916	the name Brevibacterium zhoupengii sp	1880:1916	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	6	89	theme	meso-diaminopimelic	1244:1262	arg1	meso-DAP					1270:1277	meso-DAP	1270:1277	meso-DAP	1270:1277	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	6	89	theme	meso-diaminopimelic	1244:1262	arg1	acid					1264:1267	The meso-diaminopimelic acid	1240:1267	The meso-diaminopimelic acid (meso-DAP)	1240:1278	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	6	89	theme	meso-diaminopimelic	1244:1262	arg1	acid					1307:1310	the diagnostic diamino acid	1284:1310	the diagnostic diamino acid of the peptidoglycan found in strain HY170T	1284:1354	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	7	90	theme	>	1417:1417	arg1	%					1421:1421	> 10%	1417:1421	> 10%	1417:1421	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	90	theme	>	1417:1417	arg1	acids					1410:1414	the major fatty acids	1394:1414	the major fatty acids (> 10%) of strains HY170T and HY001	1394:1450	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	9	91	theme	phenotypic	1765:1774	arg1	analyses					1776:1783	the phylogenetic, chemotaxonomic and phenotypic analyses	1728:1783	the phylogenetic, chemotaxonomic and phenotypic analyses	1728:1783	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	3	92	theme	NaCl	681:684	arg1	[w/v					686:689	0-15% NaCl [w/v	675:689	0-15% NaCl [w/v	675:689	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	3	92	theme	NaCl	681:684	arg1	range					668:672	range	668:672	range	668:672	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	4	93	dep	formed	832:837	arg1	similarity					950:959	16S rRNA similarity	941:959	16S rRNA similarity	941:959	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	7	94	theme	major	1398:1402	arg1	Anteiso-C15:0					1357:1369	Anteiso-C15:0	1357:1369	Anteiso-C15:0	1357:1369	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	94	theme	major	1398:1402	arg1	%					1421:1421	> 10%	1417:1421	> 10%	1417:1421	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	94	theme	major	1398:1402	arg1	acids					1410:1414	the major fatty acids	1394:1414	the major fatty acids (> 10%) of strains HY170T and HY001	1394:1450	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	7	94	theme	major	1398:1402	arg1	anteiso-C17:0					1375:1387	anteiso-C17:0	1375:1387	anteiso-C17:0	1375:1387	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	1	95	theme	novel	80:84	arg1	actinomycete					99:110	a novel halotolerant actinomycete	78:110	a novel halotolerant actinomycete	78:110	nov., a novel halotolerant actinomycete isolated from bat feces.
35984616	1	95	theme	novel	80:84	arg1	nov.					72:75	nov.	72:75	nov.	72:75	nov., a novel halotolerant actinomycete isolated from bat feces.
35984616	7	96	from	HY170T	1495:1500	arg1	predominant					1473:1483	predominant	1473:1483	predominant	1473:1483	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	6	97	theme	peptidoglycan	1319:1331	arg1	acid					1307:1310	the diagnostic diamino acid	1284:1310	the diagnostic diamino acid of the peptidoglycan found in strain HY170T	1284:1354	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	6	97	theme	peptidoglycan	1319:1331	arg1	acid					1264:1267	The meso-diaminopimelic acid	1240:1267	The meso-diaminopimelic acid (meso-DAP)	1240:1278	The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T.
35984616	7	98	from	absent	1506:1511	arg1	strain					1516:1521	strain HY001	1516:1527	strain HY001	1516:1527	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	2	99	theme	July	380:383	arg1	20″E					374:377	106°49'20″E	367:377	106°49'20″E	367:377	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	99	theme	July	380:383	arg1	2011					385:388	July 2011	380:388	July 2011	380:388	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	0	100	theme	Brevibacterium	42:55	arg1	sp					68:69	Brevibacterium zhoupengii sp	42:69	Brevibacterium zhoupengii sp	42:69	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.
35984616	7	101	from	strain	1516:1521	arg1	absent					1506:1511	absent	1506:1511	absent	1506:1511	Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001.
35984616	4	102	theme	core	782:785	arg1	sequences					792:800	the 16S rRNA gene and 845 core gene sequences	756:800	sequences	792:800	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	9	103	theme	phylogenetic	1732:1743	arg1	analyses					1776:1783	the phylogenetic, chemotaxonomic and phenotypic analyses	1728:1783	the phylogenetic, chemotaxonomic and phenotypic analyses	1728:1783	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	0	104	theme	sp	68:69	arg1	characteristics					23:37	Phenotypic and genomic characteristics	0:37	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.	0:70	Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp.
35984616	9	105	theme	chemotaxonomic	1746:1759	arg1	analyses					1776:1783	the phylogenetic, chemotaxonomic and phenotypic analyses	1728:1783	the phylogenetic, chemotaxonomic and phenotypic analyses	1728:1783	Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp.
35984616	2	106	theme	rod-shaped	210:219	arg1	bacteria					250:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria	137:257	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001)	137:276	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	106	theme	rod-shaped	210:219	arg1	mm					246:247	approximately 0.7 × 1.9 mm	222:247	approximately 0.7 × 1.9 mm	222:247	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	106	theme	rod-shaped	210:219	arg1	HY001					271:275	HY001	271:275	HY001	271:275	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	106	theme	rod-shaped	210:219	arg1	HY170T					260:265	HY170T	260:265	HY170T	260:265	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	4	107	theme	genus	869:873	arg1	Brevibacterium					875:888	the genus Brevibacterium	865:888	the genus Brevibacterium	865:888	The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7-46.8%; ANI, 91.9-92.1%).
35984616	3	108	theme	%	578:578	arg1	plate					592:596	BHI-5% sheep blood plate	573:596	BHI-5% sheep blood plate with pH 7.5	573:608	Optimal growth is obtained at 25-28°C (range, 4-32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0-10.0) in the presence of 0.5-1.0% NaCl (w/v) (range, 0-15% NaCl [w/v]).
35984616	2	109	dep	54″N	361:364	arg1	20″E					374:377	106°49'20″E	367:377	106°49'20″E	367:377	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35984616	2	109	dep	54″N	361:364	arg1	2011					385:388	July 2011	380:388	July 2011	380:388	Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20'54″N, 106°49'20″E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09'10″N, 102°04'39″E, October 2013) of South China, respectively.
35346471	7	0	theme	experimental	1323:1334	arg1	diets					1336:1340	experimental diets	1323:1340	experimental diets	1323:1340	Cows were individually fed a common diet during a 2-wk covariate period followed by 8 wk on experimental diets.
35346471	9	1	theme	DM	1488:1489	arg1	basis					1491:1495	a DM basis	1486:1495	a DM basis	1486:1495	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	3	2	from	levels	573:578	arg1	substitution					508:519	substitution	508:519	substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP)	508:620	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	14	3	theme	RDP	1988:1990	arg1	effects					2007:2013	No RDP or interaction effects	1985:2013	effects	2007:2013	No RDP or interaction effects were detected for these measures.
35346471	11	4	theme	milk	1776:1779	arg1	kg					1753:1754	45.5 kg	1748:1754	45.5 kg of energy-corrected milk (ECM)	1748:1785	At 10 wk on study, cows averaged 45.5 kg of energy-corrected milk (ECM).
35346471	4	5	theme	digestible	908:917	arg1	materials					859:867	nonnutritive materials	846:867	nonnutritive materials in Mol	846:874	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	4	5	theme	digestible	908:917	arg1	carbohydrate					919:930	potentially digestible carbohydrate	896:930	potentially digestible carbohydrate	896:930	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	3	6	theme	RDP	603:605	arg1	levels					593:598	2 levels	591:598	2 levels of RDP (+RDP or -RDP)	591:620	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	3	6	theme	RDP	603:605	arg1	levels					573:578	3 levels	571:578	3 levels of Mol	571:585	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	6	7	theme	nutritional	1112:1122	arg1	models					1124:1129	the nutritional models	1108:1129	the nutritional models	1108:1129	For evaluation of the nutritional models, we adopted the null hypothesis that observed and predicted lactation performance would not differ.
35346471	3	8	theme	lactating	481:489	arg1	cows					500:503	59 lactating Holstein cows	478:503	59 lactating Holstein cows	478:503	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	2	9	from	rich	308:311	arg1	starch					326:331	starch	326:331	starch	326:331	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	2	9	from	rich	308:311	arg1	sugars					316:321	sugars	316:321	sugars	316:321	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	18	10	theme	2001	2679:2682	arg1	model					2716:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	2	11	from	sugars	316:321	arg1	rich					308:311	rich	308:311	rich	308:311	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	2	12	theme	modifying	281:289	arg1	diets					291:295	modifying diets	281:295	modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP)	281:371	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	6	13	dep	hypothesis	1152:1161	arg1	differ					1223:1228	differ	1223:1228	would not differ	1213:1228	For evaluation of the nutritional models, we adopted the null hypothesis that observed and predicted lactation performance would not differ.
35346471	18	14	theme	metabolizable	2842:2854	arg1	ECM					2874:2876	metabolizable protein-allowable ECM	2842:2876	metabolizable protein-allowable ECM	2842:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	2	15	from	starch	326:331	arg1	rich					308:311	rich	308:311	rich	308:311	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	3	16	theme	cows	500:503	arg1	responses					465:473	responses	465:473	responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP)	465:620	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	18	17	theme	Council	2708:2714	arg1	model					2716:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	16	18	theme	milk	2203:2206	arg1	yield					2212:2216	milk fat yield	2203:2216	milk fat yield	2203:2216	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	9	19	theme	alfalfa	1519:1525	arg1	silage					1527:1532	alfalfa silage	1519:1532	20% alfalfa silage	1515:1532	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	2	20	theme	Little	197:202	arg1	data					204:207	Little data	197:207	Little data	197:207	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	3	21	theme	randomized	627:636	arg1	block					647:651	a randomized complete block	625:651	a randomized complete block design with a 3 × 2 factorial arrangement of treatments	625:707	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	19	22	theme	dietary	3091:3097	arg1	protein					3099:3105	dietary protein	3091:3105	dietary protein based on degradability	3091:3128	Prediction discrepancies suggest issues with valuation of dietary protein based on degradability.
35346471	18	23	theme	National	2690:2697	arg1	model					2716:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	16	24	theme	yield	2212:2216	arg1	maintenance					2188:2198	maintenance	2188:2198	maintenance	2188:2198	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	16	24	theme	yield	2212:2216	arg1	ECM/DMI					2176:2182	similar ECM/DMI	2168:2182	similar ECM/DMI	2168:2182	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	18	25	theme	actual	2893:2898	arg1	ECM					2900:2902	actual ECM	2893:2902	actual ECM for +RDP diets	2893:2917	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	19	26	theme	Prediction	3033:3042	arg1	discrepancies					3044:3056	Prediction discrepancies	3033:3056	Prediction discrepancies	3033:3056	Prediction discrepancies suggest issues with valuation of dietary protein based on degradability.
35346471	18	27	theme	ECM	2874:2876	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	9	28	contain	contained	1472:1480	arg1	diets					1466:1470	Experimental diets	1453:1470	Experimental diets	1453:1470	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	9	28	contain	contained	1472:1480	arg2	%					1500:1500	35%	1498:1500	35% corn silage	1498:1512	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	9	28	contain	contained	1472:1480	arg2	%					1543:1543	16.6%	1539:1543	16.6% crude protein	1539:1557	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	9	28	contain	contained	1472:1480	arg2	diets					1466:1470	Experimental diets	1453:1470	Experimental diets	1453:1470	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	9	28	contain	contained	1472:1480	arg2	%					1517:1517	20%	1515:1517	20% alfalfa silage	1515:1532	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	9	28	contain	contained	1472:1480	arg1	basis					1491:1495	a DM basis	1486:1495	a DM basis	1486:1495	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	6	29	theme	null	1147:1150	arg1	hypothesis					1152:1161	the null hypothesis	1143:1161	the null hypothesis that observed and predicted lactation performance would not differ	1143:1228	For evaluation of the nutritional models, we adopted the null hypothesis that observed and predicted lactation performance would not differ.
35346471	3	30	theme	cane	524:527	arg1	molasses					529:536	cane molasses	524:536	cane molasses (Mol)	524:542	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	3	30	theme	cane	524:527	arg1	Mol					539:541	Mol	539:541	Mol	539:541	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	17	31	with	post-ingestion	2453:2466	arg1	Mol					2473:2475	Mol	2473:2475	Mol compared with CG	2473:2492	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	3	32	theme	treatments	698:707	arg1	arrangement					683:693	a 3 × 2 factorial arrangement	665:693	a 3 × 2 factorial arrangement of treatments	665:707	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	4	33	theme	nutritional	751:761	arg1	models					763:768	2 nutritional models	749:768	2 nutritional models	749:768	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	3	34	theme	dry	548:550	arg1	CG					564:565	CG	564:565	CG	564:565	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	3	34	theme	dry	548:550	arg1	grain					557:561	dry corn grain	548:561	dry corn grain (CG)	548:566	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	15	35	theme	milk	2054:2057	arg1	efficiency					2070:2079	milk production efficiency	2054:2079	milk production efficiency	2054:2079	That milk production efficiency did not differ across diets suggests that DMI was a primary driver of performance.
35346471	1	36	theme	nutrition	112:120	arg1	predictions					128:138	nutrition model predictions	112:138	nutrition model predictions	112:138	I. Lactating cow performance, nutrition model predictions, and potential basis for butterfat and intake responses.
35346471	9	37	theme	crude	1545:1549	arg1	protein					1551:1557	crude protein	1545:1557	16.6% crude protein	1539:1557	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	3	38	theme	block	647:651	arg1	design					653:658	a randomized complete block design	625:658	a randomized complete block design with a 3 × 2 factorial arrangement of treatments	625:707	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	19	39	with	issues	3066:3071	arg1	valuation					3078:3086	valuation	3078:3086	valuation of dietary protein based on degradability	3078:3128	Prediction discrepancies suggest issues with valuation of dietary protein based on degradability.
35346471	17	40	theme	propionate	2641:2650	arg1	masses					2512:2517	masses	2512:2517	masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake	2512:2672	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	9	41	dep	%	1517:1517	arg1	silage					1527:1532	alfalfa silage	1519:1532	20% alfalfa silage	1515:1532	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	20	42	theme	factors	3157:3163	arg1	understanding					3140:3152	Improved understanding	3131:3152	Improved understanding of factors mediating these results	3131:3187	Improved understanding of factors mediating these results would likely enhance our ability to predict animal responses.
35346471	17	43	theme	existing	2556:2563	arg1	energy					2565:2570	existing energy	2556:2570	existing energy	2556:2570	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	18	44	theme	System	2802:2807	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	0	45	theme	protein	73:79	arg1	levels					52:57	two levels	48:57	two levels of degradable protein	48:79	Substitution of cane molasses for corn grain at two levels of degradable protein.
35346471	5	46	theme	dry	954:956	arg1	intake					970:975	dry matter (DM) intake	954:975	dry matter (DM) intake	954:975	We hypothesized that dry matter (DM) intake and milk fat percentage responses would increase with increasing Mol and would potentially be greater with +RDP.
35346471	18	47	theme	Nutritional	2782:2792	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	17	48	theme	ruminal	2411:2417	arg1	evolution					2419:2427	more rapid ruminal evolution	2400:2427	more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG	2400:2492	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	12	49	theme	milk	1856:1859	arg1	N					1870:1870	milk N/intake N	1856:1870	milk N/intake N	1856:1870	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	2	50	theme	rumen-degradable	342:357	arg1	RDP					368:370	RDP	368:370	RDP	368:370	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	2	50	theme	rumen-degradable	342:357	arg1	protein					359:365	rumen-degradable protein	342:365	rumen-degradable protein (RDP)	342:371	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	6	51	theme	lactation	1191:1199	arg1	performance					1201:1211	observed and predicted lactation performance	1168:1211	observed and predicted lactation performance	1168:1211	For evaluation of the nutritional models, we adopted the null hypothesis that observed and predicted lactation performance would not differ.
35346471	8	52	theme	similar	1398:1404	arg1	carbohydrates					1438:1450	ST and water-soluble carbohydrates	1417:1450	ST and water-soluble carbohydrates	1417:1450	Diets were formulated to be isonitrogenous and provide similar amounts of ST and water-soluble carbohydrates.
35346471	8	52	theme	similar	1398:1404	arg1	amounts					1406:1412	similar amounts	1398:1412	similar amounts of ST and water-soluble carbohydrates	1398:1450	Diets were formulated to be isonitrogenous and provide similar amounts of ST and water-soluble carbohydrates.
35346471	12	53	theme	milk	1829:1832	arg1	protein					1834:1840	milk protein	1829:1840	milk protein	1829:1840	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	19	54	theme	protein	3099:3105	arg1	valuation					3078:3086	valuation	3078:3086	valuation of dietary protein based on degradability	3078:3128	Prediction discrepancies suggest issues with valuation of dietary protein based on degradability.
35346471	18	55	from	Carbohydrate	2742:2753	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	0	56	theme	cane	16:19	arg1	molasses					21:28	cane molasses	16:28	cane molasses	16:28	Substitution of cane molasses for corn grain at two levels of degradable protein.
35346471	1	57	theme	Lactating	85:93	arg1	performance					99:109	Lactating cow performance	85:109	Lactating cow performance	85:109	I. Lactating cow performance, nutrition model predictions, and potential basis for butterfat and intake responses.
35346471	4	58	theme	nonnutritive	846:857	arg1	materials					859:867	nonnutritive materials	846:867	nonnutritive materials in Mol	846:874	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	4	58	theme	nonnutritive	846:857	arg1	carbohydrate					919:930	potentially digestible carbohydrate	896:930	potentially digestible carbohydrate	896:930	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	5	59	dep	intake	970:975	arg1	responses					1001:1009	percentage responses	990:1009	percentage responses	990:1009	We hypothesized that dry matter (DM) intake and milk fat percentage responses would increase with increasing Mol and would potentially be greater with +RDP.
35346471	18	60	theme	Dairy	2684:2688	arg1	model					2716:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	10	61	theme	water-soluble	1686:1698	arg1	carbohydrates					1700:1712	5.5, 8.5, and 11.5% water-soluble carbohydrates	1666:1712	5.5, 8.5, and 11.5% water-soluble carbohydrates	1666:1712	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	16	62	theme	fermentation	2313:2324	arg1	rates					2326:2330	fermentation rates	2313:2330	fermentation rates	2313:2330	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	2	63	theme	dairy	410:414	arg1	cows					416:419	high-producing dairy cows	395:419	high-producing dairy cows	395:419	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	18	64	theme	-RDP	2988:2991	arg1	diets					2993:2997	-RDP diets	2988:2997	-RDP diets	2988:2997	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	12	65	theme	milk	1823:1826	arg1	protein					1834:1840	milk protein	1829:1840	milk protein	1829:1840	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	12	65	theme	milk	1823:1826	arg1	intake					1795:1800	The DM intake	1788:1800	The DM intake (DMI)	1788:1806	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	12	65	theme	milk	1823:1826	arg1	N					1870:1870	milk N/intake N	1856:1870	milk N/intake N	1856:1870	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	12	65	theme	milk	1823:1826	arg1	yields					1813:1818	yields	1813:1818	yields of milk	1813:1826	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	12	65	theme	milk	1823:1826	arg1	ECM					1847:1849	ECM	1847:1849	ECM	1847:1849	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	12	65	theme	milk	1823:1826	arg1	DMI					1803:1805	DMI	1803:1805	DMI	1803:1805	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	3	66	dep	RDP	603:605	arg1	-RDP					616:619	-RDP	616:619	-RDP	616:619	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	3	66	dep	RDP	603:605	arg1	+RDP					608:611	+RDP	608:611	+RDP	608:611	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	18	67	theme	Cornell	2730:2736	arg1	Carbohydrate					2742:2753	the Cornell Net Carbohydrate	2726:2753	the Cornell Net Carbohydrate	2726:2753	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	17	68	theme	fatty	2441:2445	arg1	acids					2447:2451	volatile fatty acids	2432:2451	volatile fatty acids post-ingestion with Mol compared with CG	2432:2492	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	13	69	theme	fat	1962:1964	arg1	yield					1966:1970	milk fat yield	1957:1970	milk fat yield	1957:1970	Differences among diets were not detected for milk fat yield and ECM/DMI.
35346471	11	70	from	wk	1721:1722	arg1	study					1727:1731	study	1727:1731	study	1727:1731	At 10 wk on study, cows averaged 45.5 kg of energy-corrected milk (ECM).
35346471	8	71	theme	carbohydrates	1438:1450	arg1	carbohydrates					1438:1450	ST and water-soluble carbohydrates	1417:1450	ST and water-soluble carbohydrates	1417:1450	Diets were formulated to be isonitrogenous and provide similar amounts of ST and water-soluble carbohydrates.
35346471	8	71	theme	carbohydrates	1438:1450	arg1	amounts					1406:1412	similar amounts	1398:1412	similar amounts of ST and water-soluble carbohydrates	1398:1450	Diets were formulated to be isonitrogenous and provide similar amounts of ST and water-soluble carbohydrates.
35346471	17	72	theme	post-ingestion	2453:2466	arg1	acids					2447:2451	volatile fatty acids	2432:2451	volatile fatty acids post-ingestion with Mol compared with CG	2432:2492	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	10	73	contain	contained	1606:1614	arg2	ST					1658:1659	ST	1658:1659	ST	1658:1659	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	10	73	contain	contained	1606:1614	arg2	CG					1638:1639	19.0, 14.5, and 10.0% CG	1616:1639	19.0, 14.5, and 10.0% CG	1616:1639	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	10	73	contain	contained	1606:1614	arg1	diets					1587:1591	The 0, 5.25, and 10.5% Mol diets	1560:1591	The 0, 5.25, and 10.5% Mol diets	1560:1591	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	10	73	contain	contained	1606:1614	arg2	carbohydrates					1700:1712	5.5, 8.5, and 11.5% water-soluble carbohydrates	1666:1712	5.5, 8.5, and 11.5% water-soluble carbohydrates	1666:1712	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	10	74	theme	%	1684:1684	arg1	carbohydrates					1700:1712	5.5, 8.5, and 11.5% water-soluble carbohydrates	1666:1712	5.5, 8.5, and 11.5% water-soluble carbohydrates	1666:1712	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	16	75	theme	Mol	2257:2259	arg1	composition					2268:2278	Mol and CG composition	2257:2278	composition	2268:2278	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	4	76	from	materials	859:867	arg1	Mol					872:874	Mol	872:874	Mol	872:874	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	3	77	theme	study	444:448	arg1	objective					426:434	The objective	422:434	The objective of this study	422:448	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	18	78	theme	+RDP	2908:2911	arg1	diets					2913:2917	+RDP diets	2908:2917	+RDP diets	2908:2917	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	16	79	theme	CG	2265:2266	arg1	composition					2268:2278	Mol and CG composition	2257:2278	composition	2268:2278	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	3	80	theme	Mol	583:585	arg1	levels					593:598	2 levels	591:598	2 levels of RDP (+RDP or -RDP)	591:620	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	3	80	theme	Mol	583:585	arg1	levels					573:578	3 levels	571:578	3 levels of Mol	571:585	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	6	81	theme	models	1124:1129	arg1	evaluation					1094:1103	evaluation	1094:1103	evaluation of the nutritional models	1094:1129	For evaluation of the nutritional models, we adopted the null hypothesis that observed and predicted lactation performance would not differ.
35346471	2	82	theme	combined	262:269	arg1	effect					271:276	the combined effect	258:276	the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows	258:419	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	4	83	theme	lactation	716:724	arg1	responses					726:734	lactation responses	716:734	lactation responses predicted by 2 nutritional models	716:768	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	7	84	theme	2-wk	1281:1284	arg1	period					1296:1301	a 2-wk covariate period	1279:1301	a 2-wk covariate period followed by 8 wk on experimental diets	1279:1340	Cows were individually fed a common diet during a 2-wk covariate period followed by 8 wk on experimental diets.
35346471	1	85	theme	potential	145:153	arg1	basis					155:159	potential basis	145:159	potential basis for butterfat and intake responses	145:194	I. Lactating cow performance, nutrition model predictions, and potential basis for butterfat and intake responses.
35346471	7	86	theme	covariate	1286:1294	arg1	period					1296:1301	a 2-wk covariate period	1279:1301	a 2-wk covariate period followed by 8 wk on experimental diets	1279:1340	Cows were individually fed a common diet during a 2-wk covariate period followed by 8 wk on experimental diets.
35346471	9	87	theme	corn	1502:1505	arg1	silage					1507:1512	corn silage	1502:1512	35% corn silage	1498:1512	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	10	88	dep	diets	1587:1591	arg1	%					1581:1581	%	1581:1581	%	1581:1581	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	3	89	from	levels	593:598	arg1	substitution					508:519	substitution	508:519	substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP)	508:620	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	2	90	theme	diets	291:295	arg1	effect					271:276	the combined effect	258:276	the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows	258:419	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	7	91	theme	common	1260:1265	arg1	diet					1267:1270	a common diet	1258:1270	a common diet	1258:1270	Cows were individually fed a common diet during a 2-wk covariate period followed by 8 wk on experimental diets.
35346471	12	92	theme	DM	1792:1793	arg1	DMI					1803:1805	DMI	1803:1805	DMI	1803:1805	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	12	92	theme	DM	1792:1793	arg1	intake					1795:1800	The DM intake	1788:1800	The DM intake (DMI)	1788:1806	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	6	93	theme	predicted	1181:1189	arg1	performance					1201:1211	observed and predicted lactation performance	1168:1211	observed and predicted lactation performance	1168:1211	For evaluation of the nutritional models, we adopted the null hypothesis that observed and predicted lactation performance would not differ.
35346471	3	94	theme	Holstein	491:498	arg1	cows					500:503	59 lactating Holstein cows	478:503	59 lactating Holstein cows	478:503	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	17	95	from	excess	2546:2551	arg1	masses					2512:2517	masses	2512:2517	masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake	2512:2672	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	16	96	theme	fat	2208:2210	arg1	yield					2212:2216	milk fat yield	2203:2216	milk fat yield	2203:2216	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	6	97	theme	observed	1168:1175	arg1	performance					1201:1211	observed and predicted lactation performance	1168:1211	observed and predicted lactation performance	1168:1211	For evaluation of the nutritional models, we adopted the null hypothesis that observed and predicted lactation performance would not differ.
35346471	3	98	theme	molasses	529:536	arg1	substitution					508:519	substitution	508:519	substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP)	508:620	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	18	99	theme	Research	2699:2706	arg1	model					2716:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model	2675:2720	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	7	100	from	wk	1317:1318	arg1	diets					1336:1340	experimental diets	1323:1340	experimental diets	1323:1340	Cows were individually fed a common diet during a 2-wk covariate period followed by 8 wk on experimental diets.
35346471	0	101	from	levels	52:57	arg1	Substitution					0:11	Substitution	0:11	Substitution of cane molasses for corn grain at two levels of degradable protein	0:79	Substitution of cane molasses for corn grain at two levels of degradable protein.
35346471	10	102	dep	%	1581:1581	arg1	5.25					1567:1570	5.25	1567:1570	5.25	1567:1570	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	10	102	dep	%	1581:1581	arg1	10.5					1577:1580	10.5	1577:1580	10.5	1577:1580	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	3	103	theme	complete	638:645	arg1	block					647:651	a randomized complete block	625:651	a randomized complete block design with a 3 × 2 factorial arrangement of treatments	625:707	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	17	104	from	acetate	2522:2528	arg1	excess					2546:2551	excess	2546:2551	excess of existing energy and synthetic needs that were shunted to milk fat production	2546:2631	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	1	105	theme	cow	95:97	arg1	performance					99:109	Lactating cow performance	85:109	Lactating cow performance	85:109	I. Lactating cow performance, nutrition model predictions, and potential basis for butterfat and intake responses.
35346471	15	106	theme	production	2059:2068	arg1	efficiency					2070:2079	milk production efficiency	2054:2079	milk production efficiency	2054:2079	That milk production efficiency did not differ across diets suggests that DMI was a primary driver of performance.
35346471	11	107	theme	energy-corrected	1759:1774	arg1	ECM					1782:1784	ECM	1782:1784	ECM	1782:1784	At 10 wk on study, cows averaged 45.5 kg of energy-corrected milk (ECM).
35346471	11	107	theme	energy-corrected	1759:1774	arg1	milk					1776:1779	energy-corrected milk	1759:1779	energy-corrected milk (ECM)	1759:1785	At 10 wk on study, cows averaged 45.5 kg of energy-corrected milk (ECM).
35346471	17	108	from	masses	2512:2517	arg1	excess					2546:2551	excess	2546:2551	excess of existing energy and synthetic needs that were shunted to milk fat production	2546:2631	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	17	109	dep	synthetic	2576:2584	arg1	needs					2586:2590	needs	2586:2590	needs	2586:2590	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	17	110	theme	milk	2613:2616	arg1	fat					2618:2620	milk fat	2613:2620	milk fat production	2613:2631	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	18	111	theme	protein-allowable	2856:2872	arg1	ECM					2874:2876	metabolizable protein-allowable ECM	2842:2876	metabolizable protein-allowable ECM	2842:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	17	112	theme	energy	2565:2570	arg1	excess					2546:2551	excess	2546:2551	excess of existing energy and synthetic needs that were shunted to milk fat production	2546:2631	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	3	113	with	design	653:658	arg1	arrangement					683:693	a 3 × 2 factorial arrangement	665:693	a 3 × 2 factorial arrangement of treatments	665:707	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	1	114	theme	model	122:126	arg1	predictions					128:138	nutrition model predictions	112:138	nutrition model predictions	112:138	I. Lactating cow performance, nutrition model predictions, and potential basis for butterfat and intake responses.
35346471	4	115	theme	Mol	814:816	arg1	composition					818:828	Mol composition	814:828	Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate	814:930	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	9	116	dep	%	1543:1543	arg1	protein					1551:1557	crude protein	1545:1557	16.6% crude protein	1539:1557	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	0	117	theme	degradable	62:71	arg1	protein					73:79	degradable protein	62:79	degradable protein	62:79	Substitution of cane molasses for corn grain at two levels of degradable protein.
35346471	16	118	from	differences	2298:2308	arg1	products					2336:2343	products	2336:2343	products	2336:2343	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	16	118	from	differences	2298:2308	arg1	rates					2326:2330	fermentation rates	2313:2330	fermentation rates	2313:2330	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	2	119	from	effect	271:276	arg1	performance					380:390	the performance	376:390	the performance of high-producing dairy cows	376:419	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	16	120	theme	similar	2168:2174	arg1	ECM/DMI					2176:2182	similar ECM/DMI	2168:2182	similar ECM/DMI	2168:2182	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	3	121	theme	factorial	673:681	arg1	arrangement					683:693	a 3 × 2 factorial arrangement	665:693	a 3 × 2 factorial arrangement of treatments	665:707	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	5	122	theme	milk	981:984	arg1	fat					986:988	milk fat	981:988	milk fat	981:988	We hypothesized that dry matter (DM) intake and milk fat percentage responses would increase with increasing Mol and would potentially be greater with +RDP.
35346471	15	123	theme	primary	2133:2139	arg1	driver					2141:2146	a primary driver	2131:2146	a primary driver of performance	2131:2161	That milk production efficiency did not differ across diets suggests that DMI was a primary driver of performance.
35346471	15	123	theme	primary	2133:2139	arg1	DMI					2123:2125	DMI	2123:2125	DMI	2123:2125	That milk production efficiency did not differ across diets suggests that DMI was a primary driver of performance.
35346471	18	124	theme	Dynamic	2794:2800	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	17	125	theme	fat	2618:2620	arg1	production					2622:2631	milk fat production	2613:2631	milk fat production	2613:2631	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	4	126	theme	observed	789:796	arg1	responses					798:806	observed responses	789:806	observed responses	789:806	Also, lactation responses predicted by 2 nutritional models were compared with observed responses, with Mol composition entered so that nonnutritive materials in Mol were not counted as potentially digestible carbohydrate.
35346471	12	127	theme	N/intake	1861:1868	arg1	N					1870:1870	milk N/intake N	1856:1870	milk N/intake N	1856:1870	The DM intake (DMI), and yields of milk, milk protein, and ECM, and milk N/intake N declined linearly with increasing Mol.
35346471	3	128	theme	×	669:669	arg1	arrangement					683:693	a 3 × 2 factorial arrangement	665:693	a 3 × 2 factorial arrangement of treatments	665:707	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	5	129	theme	matter	958:963	arg1	intake					970:975	dry matter (DM) intake	954:975	dry matter (DM) intake	954:975	We hypothesized that dry matter (DM) intake and milk fat percentage responses would increase with increasing Mol and would potentially be greater with +RDP.
35346471	20	130	theme	Improved	3131:3138	arg1	understanding					3140:3152	Improved understanding	3131:3152	Improved understanding of factors mediating these results	3131:3187	Improved understanding of factors mediating these results would likely enhance our ability to predict animal responses.
35346471	18	131	theme	Protein	2759:2765	arg1	System					2767:2772	Protein System 6.55	2759:2777	Protein System 6.55	2759:2777	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	2	132	dep	starch	326:331	arg1	ST					334:335	ST	334:335	ST	334:335	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	17	133	theme	synthetic	2576:2584	arg1	excess					2546:2551	excess	2546:2551	excess of existing energy and synthetic needs that were shunted to milk fat production	2546:2631	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	5	134	theme	DM	966:967	arg1	intake					970:975	dry matter (DM) intake	954:975	dry matter (DM) intake	954:975	We hypothesized that dry matter (DM) intake and milk fat percentage responses would increase with increasing Mol and would potentially be greater with +RDP.
35346471	18	135	theme	2021	2823:2826	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	0	136	theme	molasses	21:28	arg1	Substitution					0:11	Substitution	0:11	Substitution of cane molasses for corn grain at two levels of degradable protein	0:79	Substitution of cane molasses for corn grain at two levels of degradable protein.
35346471	18	137	theme	Professional	2809:2820	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	10	138	theme	Mol	1583:1585	arg1	diets					1587:1591	The 0, 5.25, and 10.5% Mol diets	1560:1591	The 0, 5.25, and 10.5% Mol diets	1560:1591	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	18	139	from	System	2767:2772	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	0	140	theme	corn	34:37	arg1	grain					39:43	corn grain	34:43	corn grain	34:43	Substitution of cane molasses for corn grain at two levels of degradable protein.
35346471	20	141	theme	animal	3233:3238	arg1	responses					3240:3248	animal responses	3233:3248	animal responses	3233:3248	Improved understanding of factors mediating these results would likely enhance our ability to predict animal responses.
35346471	18	142	theme	Net	2738:2740	arg1	Carbohydrate					2742:2753	the Cornell Net Carbohydrate	2726:2753	the Cornell Net Carbohydrate	2726:2753	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	2	143	theme	cows	416:419	arg1	performance					380:390	the performance	376:390	the performance of high-producing dairy cows	376:419	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	8	144	theme	ST	1417:1418	arg1	carbohydrates					1438:1450	ST and water-soluble carbohydrates	1417:1450	ST and water-soluble carbohydrates	1417:1450	Diets were formulated to be isonitrogenous and provide similar amounts of ST and water-soluble carbohydrates.
35346471	3	145	theme	corn	552:555	arg1	CG					564:565	CG	564:565	CG	564:565	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	3	145	theme	corn	552:555	arg1	grain					557:561	dry corn grain	548:561	dry corn grain (CG)	548:566	The objective of this study was to compare responses of 59 lactating Holstein cows to substitution of cane molasses (Mol) for dry corn grain (CG) at 3 levels of Mol and 2 levels of RDP (+RDP or -RDP) in a randomized complete block design with a 3 × 2 factorial arrangement of treatments.
35346471	1	146	dep	butterfat	165:173	arg1	responses					186:194	responses	186:194	responses	186:194	I. Lactating cow performance, nutrition model predictions, and potential basis for butterfat and intake responses.
35346471	17	147	theme	acetate	2522:2528	arg1	masses					2512:2517	masses	2512:2517	masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake	2512:2672	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	9	148	dep	%	1500:1500	arg1	silage					1507:1512	corn silage	1502:1512	35% corn silage	1498:1512	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	5	149	theme	percentage	990:999	arg1	responses					1001:1009	percentage responses	990:1009	percentage responses	990:1009	We hypothesized that dry matter (DM) intake and milk fat percentage responses would increase with increasing Mol and would potentially be greater with +RDP.
35346471	9	150	theme	Experimental	1453:1464	arg1	diets					1466:1470	Experimental diets	1453:1470	Experimental diets	1453:1470	Experimental diets contained, on a DM basis, 35% corn silage, 20% alfalfa silage, and 16.6% crude protein.
35346471	2	151	theme	high-producing	395:408	arg1	cows					416:419	high-producing dairy cows	395:419	high-producing dairy cows	395:419	Little data is presently available on our ability to predict the combined effect of modifying diets with feeds rich in sugars or starch (ST) and rumen-degradable protein (RDP) on the performance of high-producing dairy cows.
35346471	8	152	theme	water-soluble	1424:1436	arg1	carbohydrates					1438:1450	ST and water-soluble carbohydrates	1417:1450	ST and water-soluble carbohydrates	1417:1450	Diets were formulated to be isonitrogenous and provide similar amounts of ST and water-soluble carbohydrates.
35346471	17	153	theme	volatile	2432:2439	arg1	acids					2447:2451	volatile fatty acids	2432:2451	volatile fatty acids post-ingestion with Mol compared with CG	2432:2492	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	17	154	theme	rapid	2405:2409	arg1	evolution					2419:2427	more rapid ruminal evolution	2400:2427	more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG	2400:2492	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	13	155	theme	milk	1957:1960	arg1	yield					1966:1970	milk fat yield	1957:1970	milk fat yield	1957:1970	Differences among diets were not detected for milk fat yield and ECM/DMI.
35346471	18	156	from	model	2716:2720	arg1	estimates					2829:2837	Nutritional Dynamic System Professional (2021) estimates	2782:2837	Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM	2782:2876	The 2001 Dairy National Research Council model and the Cornell Net Carbohydrate and Protein System 6.55 in Nutritional Dynamic System Professional (2021) estimates of metabolizable protein-allowable ECM underestimated actual ECM for +RDP diets by 4.5 and 2.3 kg, respectively, and came close or overestimated for -RDP diets by 0.25 and 5.0 kg, respectively.
35346471	10	157	theme	%	1636:1636	arg1	CG					1638:1639	19.0, 14.5, and 10.0% CG	1616:1639	19.0, 14.5, and 10.0% CG	1616:1639	The 0, 5.25, and 10.5% Mol diets respectively contained 19.0, 14.5, and 10.0% CG; 28, 25, and 22% ST; and 5.5, 8.5, and 11.5% water-soluble carbohydrates.
35346471	17	158	theme	acids	2447:2451	arg1	evolution					2419:2427	more rapid ruminal evolution	2400:2427	more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG	2400:2492	As explanation for these results, we hypothesize that more rapid ruminal evolution of volatile fatty acids post-ingestion with Mol compared with CG may have provided masses of acetate and butyrate in excess of existing energy and synthetic needs that were shunted to milk fat production, and of propionate that depressed intake.
35346471	16	159	dep	ECM/DMI	2176:2182	arg1	The					2164:2166	The	2164:2166	The	2164:2166	The similar ECM/DMI and maintenance of milk fat yield would not have been predicted based on Mol and CG composition but may relate to differences in fermentation rates and products.
35346471	14	160	theme	interaction	1995:2005	arg1	effects					2007:2013	No RDP or interaction effects	1985:2013	effects	2007:2013	No RDP or interaction effects were detected for these measures.
35346471	15	161	theme	performance	2151:2161	arg1	driver					2141:2146	a primary driver	2131:2146	a primary driver of performance	2131:2161	That milk production efficiency did not differ across diets suggests that DMI was a primary driver of performance.
35346471	15	161	theme	performance	2151:2161	arg1	DMI					2123:2125	DMI	2123:2125	DMI	2123:2125	That milk production efficiency did not differ across diets suggests that DMI was a primary driver of performance.
36566464	10	0	theme	weaning	1277:1283	arg1	weight					1285:1290	weaning weight	1277:1290	weaning weight	1277:1290	Mitochondrial DNA CN tended (P-value ≤0.08) to be associated with birth weight and weaning weight.
36566464	1	1	theme	low-pass	217:224	arg1	LPS					238:240	LPS	238:240	LPS	238:240	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	1	1	theme	low-pass	217:224	arg1	sequencing					226:235	low-pass sequencing	217:235	low-pass sequencing (LPS)	217:241	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	12	2	theme	average	1575:1581	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	9	3	theme	univariate	1079:1088	arg1	models					1097:1102	univariate animal models	1079:1102	univariate animal models	1079:1102	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	12	4	theme	carcass	1771:1777	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	5	theme	dry-matter	1589:1598	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	1	6	theme	copy	156:159	arg1	number					161:166	Mitochondrial DNA copy number	138:166	Mitochondrial DNA copy number (mtDNA CN)	138:177	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	1	6	theme	copy	156:159	arg1	CN					175:176	mtDNA CN	169:176	mtDNA CN	169:176	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	12	7	theme	daily	1583:1587	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	5	8	theme	Variant	620:626	arg1	calling					628:634	Variant calling	620:634	Variant calling	620:634	Variant calling was performed from mtDNA, and 11 single nucleotide polymorphisms (SNP) were identified in the population.
36566464	12	9	with	±	1793:1793	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	9	with	±	1793:1793	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	3	10	theme	nuclear	497:503	arg1	nuDNA					510:514	nuDNA	510:514	nuDNA	510:514	Blood, leucocyte, and semen samples were obtained from 2,371 animals and subjected to LPS that resulted in nuclear DNA (nuDNA) and mtDNA sequence reads.
36566464	3	10	theme	nuclear	497:503	arg1	DNA					505:507	nuclear DNA	497:507	nuclear DNA (nuDNA)	497:515	Blood, leucocyte, and semen samples were obtained from 2,371 animals and subjected to LPS that resulted in nuclear DNA (nuDNA) and mtDNA sequence reads.
36566464	12	11	with	±	1614:1614	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	11	with	±	1614:1614	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	12	with	±	1652:1652	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	3	13	theme	mtDNA	521:525	arg1	sequence					527:534	mtDNA sequence	521:534	mtDNA sequence reads	521:540	Blood, leucocyte, and semen samples were obtained from 2,371 animals and subjected to LPS that resulted in nuclear DNA (nuDNA) and mtDNA sequence reads.
36566464	12	14	theme	mtDNA	1440:1444	arg1	CN					1446:1447	mtDNA CN	1440:1447	mtDNA CN	1440:1447	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	0	15	theme	beef	125:128	arg1	cattle					130:135	beef cattle	125:135	beef cattle	125:135	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	13	16	theme	carcass	2009:2015	arg1	traits					2017:2022	carcass traits	2009:2022	carcass traits	2009:2022	In conclusion, mtDNA CN was negatively correlated with most traits investigated, and the genetic correlation was stronger with growth traits than with carcass traits.
36566464	12	17	theme	post-weaning	1538:1549	arg1	gain					1551:1554	post-weaning gain	1538:1554	post-weaning gain	1538:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	11	18	with	association	1306:1316	arg1	traits					1399:1404	growth and carcass traits	1380:1404	growth and carcass traits	1380:1404	There was no association (P-value >0.10) between mtDNA SNP, haplogroups, or types with growth and carcass traits.
36566464	9	19	theme	animal	1162:1167	arg1	model					1169:1173	a bivariate animal model	1150:1173	a bivariate animal model based on pedigree	1150:1191	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	11	20	theme	mtDNA	1342:1346	arg1	SNP					1348:1350	mtDNA SNP	1342:1350	mtDNA SNP	1342:1350	There was no association (P-value >0.10) between mtDNA SNP, haplogroups, or types with growth and carcass traits.
36566464	12	21	theme	Genetic	1407:1413	arg1	estimates					1427:1435	Genetic correlation estimates	1407:1435	Genetic correlation estimates of mtDNA CN	1407:1447	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	0	22	theme	number	62:67	arg1	associations					23:34	Genetic and phenotypic associations	0:34	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.	0:136	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	13	23	theme	growth	1985:1990	arg1	traits					1992:1997	growth traits	1985:1997	growth traits	1985:1997	In conclusion, mtDNA CN was negatively correlated with most traits investigated, and the genetic correlation was stronger with growth traits than with carcass traits.
36566464	11	24	theme	growth	1380:1385	arg1	traits					1399:1404	growth and carcass traits	1380:1404	growth and carcass traits	1380:1404	There was no association (P-value >0.10) between mtDNA SNP, haplogroups, or types with growth and carcass traits.
36566464	0	25	theme	SNP	70:72	arg1	associations					23:34	Genetic and phenotypic associations	0:34	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.	0:136	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	9	26	with	Associations	956:967	arg1	traits					1052:1057	growth and carcass traits	1033:1057	growth and carcass traits	1033:1057	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	1	27	theme	Mitochondrial	138:150	arg1	number					161:166	Mitochondrial DNA copy number	138:166	Mitochondrial DNA copy number (mtDNA CN)	138:177	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	1	27	theme	Mitochondrial	138:150	arg1	CN					175:176	mtDNA CN	169:176	mtDNA CN	169:176	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	9	28	theme	CN	978:979	arg1	Associations					956:967	Associations	956:967	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits	956:1057	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	7	29	theme	segregating	856:866	arg1	SNP					868:870	the 11 segregating SNP	849:870	the 11 segregating SNP	849:870	Haplogroup and mtDNA type were further classified based on the 11 segregating SNP.
36566464	2	30	theme	beef	366:369	arg1	population					378:387	a multi-breed and crossbred beef cattle population	338:387	a multi-breed and crossbred beef cattle population	338:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	9	31	theme	mtDNA	982:986	arg1	haplogroups					988:998	mtDNA haplogroups	982:998	mtDNA haplogroups	982:998	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	2	32	theme	multi-breed	340:350	arg1	population					378:387	a multi-breed and crossbred beef cattle population	338:387	a multi-breed and crossbred beef cattle population	338:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	12	33	with	±	1725:1725	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	33	with	±	1725:1725	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	0	34	theme	Genetic	0:6	arg1	associations					23:34	Genetic and phenotypic associations	0:34	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.	0:136	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	13	35	theme	genetic	1947:1953	arg1	correlation					1955:1965	the genetic correlation	1943:1965	the genetic correlation	1943:1965	In conclusion, mtDNA CN was negatively correlated with most traits investigated, and the genetic correlation was stronger with growth traits than with carcass traits.
36566464	13	35	theme	genetic	1947:1953	arg1	stronger					1971:1978	stronger	1971:1978	stronger	1971:1978	In conclusion, mtDNA CN was negatively correlated with most traits investigated, and the genetic correlation was stronger with growth traits than with carcass traits.
36566464	12	36	with	±	1526:1526	arg1	gain					1551:1554	post-weaning gain	1538:1554	post-weaning gain	1538:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	6	37	theme	taurine	769:775	arg1	haplogroups					777:787	taurine haplogroups	769:787	taurine haplogroups	769:787	Samples were classified in taurine haplogroups.
36566464	9	38	theme	animal	1090:1095	arg1	models					1097:1102	univariate animal models	1079:1102	univariate animal models	1079:1102	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	9	39	theme	types	1007:1011	arg1	Associations					956:967	Associations	956:967	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits	956:1057	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	2	40	theme	cattle	371:376	arg1	population					378:387	a multi-breed and crossbred beef cattle population	338:387	a multi-breed and crossbred beef cattle population	338:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	0	41	theme	phenotypic	12:21	arg1	associations					23:34	Genetic and phenotypic associations	0:34	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.	0:136	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	10	42	theme	Mitochondrial	1194:1206	arg1	CN					1212:1213	Mitochondrial DNA CN	1194:1213	Mitochondrial DNA CN	1194:1213	Mitochondrial DNA CN tended (P-value ≤0.08) to be associated with birth weight and weaning weight.
36566464	2	43	theme	crossbred	356:364	arg1	population					378:387	a multi-breed and crossbred beef cattle population	338:387	a multi-breed and crossbred beef cattle population	338:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	12	44	theme	cow	1671:1673	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	45	theme	average	1626:1632	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	9	46	theme	SNP	1024:1026	arg1	Associations					956:967	Associations	956:967	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits	956:1057	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	2	47	theme	CN	301:302	arg1	correlation					280:290	the genetic correlation	268:290	the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population	268:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	0	48	theme	DNA	53:55	arg1	number					62:67	mitochondrial DNA copy number	39:67	mitochondrial DNA copy number	39:67	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	9	49	theme	growth	1033:1038	arg1	traits					1052:1057	growth and carcass traits	1033:1057	growth and carcass traits	1033:1057	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	9	50	theme	mtDNA	1018:1022	arg1	SNP					1024:1026	mtDNA SNP	1018:1026	mtDNA SNP	1018:1026	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	0	51	from	traits	115:120	arg1	cattle					130:135	beef cattle	125:135	beef cattle	125:135	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	9	52	theme	carcass	1044:1050	arg1	traits					1052:1057	growth and carcass traits	1033:1057	growth and carcass traits	1033:1057	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	2	53	theme	carcass	320:326	arg1	traits					328:333	growth and carcass traits	309:333	growth and carcass traits in a multi-breed and crossbred beef cattle population	309:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	13	54	theme	most	1913:1916	arg1	traits					1918:1923	most traits	1913:1923	most traits investigated	1913:1936	In conclusion, mtDNA CN was negatively correlated with most traits investigated, and the genetic correlation was stronger with growth traits than with carcass traits.
36566464	8	55	theme	Growth	873:878	arg1	traits					892:897	Growth and carcass traits	873:897	Growth and carcass traits	873:897	Growth and carcass traits were available for between 7,249 and 60,989 individuals.
36566464	2	56	theme	growth	309:314	arg1	traits					328:333	growth and carcass traits	309:333	growth and carcass traits in a multi-breed and crossbred beef cattle population	309:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	7	57	theme	mtDNA	805:809	arg1	type					811:814	mtDNA type	805:814	mtDNA type	805:814	Haplogroup and mtDNA type were further classified based on the 11 segregating SNP.
36566464	13	58	theme	mtDNA	1873:1877	arg1	CN					1879:1880	mtDNA CN	1873:1880	mtDNA CN	1873:1880	In conclusion, mtDNA CN was negatively correlated with most traits investigated, and the genetic correlation was stronger with growth traits than with carcass traits.
36566464	8	59	theme	carcass	884:890	arg1	traits					892:897	Growth and carcass traits	873:897	Growth and carcass traits	873:897	Growth and carcass traits were available for between 7,249 and 60,989 individuals.
36566464	12	60	with	±	1460:1460	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	60	with	±	1460:1460	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	10	61	theme	birth	1260:1264	arg1	weight					1266:1271	birth weight	1260:1271	birth weight	1260:1271	Mitochondrial DNA CN tended (P-value ≤0.08) to be associated with birth weight and weaning weight.
36566464	1	62	theme	DNA	152:154	arg1	number					161:166	Mitochondrial DNA copy number	138:166	Mitochondrial DNA copy number (mtDNA CN)	138:177	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	1	62	theme	DNA	152:154	arg1	CN					175:176	mtDNA CN	169:176	mtDNA CN	169:176	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	5	63	theme	nucleotide	676:685	arg1	SNP					702:704	SNP	702:704	SNP	702:704	Variant calling was performed from mtDNA, and 11 single nucleotide polymorphisms (SNP) were identified in the population.
36566464	5	63	theme	nucleotide	676:685	arg1	polymorphisms					687:699	11 single nucleotide polymorphisms	666:699	11 single nucleotide polymorphisms (SNP)	666:705	Variant calling was performed from mtDNA, and 11 single nucleotide polymorphisms (SNP) were identified in the population.
36566464	2	64	theme	genetic	272:278	arg1	correlation					280:290	the genetic correlation	268:290	the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population	268:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	10	65	dep	associated	1244:1253	arg1	P-value					1223:1229	P-value	1223:1229	P-value	1223:1229	Mitochondrial DNA CN tended (P-value ≤0.08) to be associated with birth weight and weaning weight.
36566464	12	66	theme	carcass	1737:1743	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	0	67	theme	carcass	107:113	arg1	traits					115:120	growth and carcass traits	96:120	growth and carcass traits in beef cattle	96:135	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	9	68	theme	genetic	1109:1115	arg1	correlations					1117:1128	genetic correlations	1109:1128	genetic correlations	1109:1128	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	3	69	dep	sequence	527:534	arg1	reads					536:540	reads	536:540	reads	536:540	Blood, leucocyte, and semen samples were obtained from 2,371 animals and subjected to LPS that resulted in nuclear DNA (nuDNA) and mtDNA sequence reads.
36566464	12	70	theme	birth	1472:1476	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	70	theme	birth	1472:1476	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	70	theme	birth	1472:1476	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	70	theme	birth	1472:1476	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	71	theme	carcass	1805:1811	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	4	72	theme	DNA	557:559	arg1	ratio					585:589	the ratio	581:589	the ratio of mtDNA to nuDNA coverages	581:617	Mitochondrial DNA CN was estimated as the ratio of mtDNA to nuDNA coverages.
36566464	4	72	theme	DNA	557:559	arg1	CN					561:562	Mitochondrial DNA CN	543:562	Mitochondrial DNA CN	543:562	Mitochondrial DNA CN was estimated as the ratio of mtDNA to nuDNA coverages.
36566464	12	73	theme	CN	1446:1447	arg1	estimates					1427:1435	Genetic correlation estimates	1407:1435	Genetic correlation estimates of mtDNA CN	1407:1447	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	74	with	±	1833:1833	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	75	theme	ribeye	1845:1850	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	9	76	theme	bivariate	1152:1160	arg1	model					1169:1173	a bivariate animal model	1150:1173	a bivariate animal model based on pedigree	1150:1191	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	12	77	theme	correlation	1415:1425	arg1	estimates					1427:1435	Genetic correlation estimates	1407:1435	Genetic correlation estimates of mtDNA CN	1407:1447	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	78	with	±	1759:1759	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	1	79	theme	mtDNA	169:173	arg1	number					161:166	Mitochondrial DNA copy number	138:166	Mitochondrial DNA copy number (mtDNA CN)	138:177	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	1	79	theme	mtDNA	169:173	arg1	CN					175:176	mtDNA CN	169:176	mtDNA CN	169:176	Mitochondrial DNA copy number (mtDNA CN) is heritable and easily obtained from low-pass sequencing (LPS).
36566464	4	80	theme	mtDNA	594:598	arg1	ratio					585:589	the ratio	581:589	the ratio of mtDNA to nuDNA coverages	581:617	Mitochondrial DNA CN was estimated as the ratio of mtDNA to nuDNA coverages.
36566464	4	80	theme	mtDNA	594:598	arg1	CN					561:562	Mitochondrial DNA CN	543:562	Mitochondrial DNA CN	543:562	Mitochondrial DNA CN was estimated as the ratio of mtDNA to nuDNA coverages.
36566464	5	81	theme	single	669:674	arg1	SNP					702:704	SNP	702:704	SNP	702:704	Variant calling was performed from mtDNA, and 11 single nucleotide polymorphisms (SNP) were identified in the population.
36566464	5	81	theme	single	669:674	arg1	polymorphisms					687:699	11 single nucleotide polymorphisms	666:699	11 single nucleotide polymorphisms (SNP)	666:705	Variant calling was performed from mtDNA, and 11 single nucleotide polymorphisms (SNP) were identified in the population.
36566464	12	82	theme	slaughter	1701:1709	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	4	83	theme	nuDNA	603:607	arg1	coverages					609:617	nuDNA coverages	603:617	nuDNA coverages	603:617	Mitochondrial DNA CN was estimated as the ratio of mtDNA to nuDNA coverages.
36566464	9	84	theme	mtDNA	972:976	arg1	CN					978:979	mtDNA CN	972:979	mtDNA CN	972:979	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	0	85	theme	haplogroups	79:89	arg1	associations					23:34	Genetic and phenotypic associations	0:34	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.	0:136	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	0	86	theme	growth	96:101	arg1	traits					115:120	growth and carcass traits	96:120	growth and carcass traits in beef cattle	96:135	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	9	87	theme	haplogroups	988:998	arg1	Associations					956:967	Associations	956:967	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits	956:1057	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	12	88	theme	weaning	1504:1510	arg1	weight					1512:1517	weaning weight	1504:1517	weaning weight	1504:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	9	89	theme	mtDNA	1001:1005	arg1	types					1007:1011	mtDNA types	1001:1011	mtDNA types	1001:1011	Associations of mtDNA CN, mtDNA haplogroups, mtDNA types, and mtDNA SNP with growth and carcass traits were estimated with univariate animal models, and genetic correlations were estimated with a bivariate animal model based on pedigree.
36566464	12	90	theme	mature	1664:1669	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	10	91	theme	DNA	1208:1210	arg1	CN					1212:1213	Mitochondrial DNA CN	1194:1213	Mitochondrial DNA CN	1194:1213	Mitochondrial DNA CN tended (P-value ≤0.08) to be associated with birth weight and weaning weight.
36566464	3	92	theme	semen	412:416	arg1	samples					418:424	Blood, leucocyte, and semen samples	390:424	samples	418:424	Blood, leucocyte, and semen samples were obtained from 2,371 animals and subjected to LPS that resulted in nuclear DNA (nuDNA) and mtDNA sequence reads.
36566464	0	93	theme	mitochondrial	39:51	arg1	number					62:67	mitochondrial DNA copy number	39:67	mitochondrial DNA copy number	39:67	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	2	94	with	correlation	280:290	arg1	traits					328:333	growth and carcass traits	309:333	growth and carcass traits in a multi-breed and crossbred beef cattle population	309:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	3	95	theme	Blood	390:394	arg1	leucocyte					397:405	Blood, leucocyte, and semen samples	390:424	leucocyte	397:405	Blood, leucocyte, and semen samples were obtained from 2,371 animals and subjected to LPS that resulted in nuclear DNA (nuDNA) and mtDNA sequence reads.
36566464	0	96	with	associations	23:34	arg1	traits					115:120	growth and carcass traits	96:120	growth and carcass traits in beef cattle	96:135	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	0	97	theme	copy	57:60	arg1	number					62:67	mitochondrial DNA copy number	39:67	mitochondrial DNA copy number	39:67	Genetic and phenotypic associations of mitochondrial DNA copy number, SNP, and haplogroups with growth and carcass traits in beef cattle.
36566464	12	98	with	±	1689:1689	arg1	weight					1478:1483	birth weight	1472:1483	birth weight	1472:1483	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	±					1492:1492	-0.31 ± 0.16	1486:1497	-0.31 ± 0.16 with weaning weight	1486:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	weight					1711:1716	slaughter weight	1701:1716	slaughter weight	1701:1716	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	weight					1745:1750	carcass weight	1737:1750	carcass weight	1737:1750	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	marbling					1813:1820	carcass marbling	1805:1820	carcass marbling	1805:1820	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	backfat					1779:1785	carcass backfat	1771:1785	carcass backfat	1771:1785	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	intake					1600:1605	average daily dry-matter intake	1575:1605	average daily dry-matter intake	1575:1605	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	±					1526:1526	-0.15 ± 0.14	1520:1531	-0.15 ± 0.14 with post-weaning gain	1520:1554	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	area					1852:1855	ribeye area	1845:1855	ribeye area	1845:1855	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	±					1563:1563	-0.11 ± 0.19	1557:1568	-0.11 ± 0.19	1557:1568	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	98	with	±	1689:1689	arg1	weight					1675:1680	mature cow weight	1664:1680	mature cow weight	1664:1680	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	2	99	from	traits	328:333	arg1	population					378:387	a multi-breed and crossbred beef cattle population	338:387	a multi-breed and crossbred beef cattle population	338:387	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	2	100	theme	mtDNA	295:299	arg1	CN					301:302	mtDNA CN	295:302	mtDNA CN	295:302	This study investigated the genetic correlation of mtDNA CN with growth and carcass traits in a multi-breed and crossbred beef cattle population.
36566464	12	101	with	±	1492:1492	arg1	weight					1512:1517	weaning weight	1504:1517	weaning weight	1504:1517	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	12	102	theme	daily	1634:1638	arg1	gain					1640:1643	average daily gain	1626:1643	average daily gain	1626:1643	Genetic correlation estimates of mtDNA CN were -0.30 ± 0.16 with birth weight, -0.31 ± 0.16 with weaning weight, -0.15 ± 0.14 with post-weaning gain, -0.11 ± 0.19 with average daily dry-matter intake, -0.04 ± 0.22 with average daily gain, -0.29 ± 0.13 with mature cow weight, -0.11 ± 0.13 with slaughter weight, -0.14 ± 0.13 with carcass weight, -0.07 ± 0.14 with carcass backfat, 0.14 ± 0.14 with carcass marbling, and -0.06 ± 0.14 with ribeye area.
36566464	4	103	theme	Mitochondrial	543:555	arg1	ratio					585:589	the ratio	581:589	the ratio of mtDNA to nuDNA coverages	581:617	Mitochondrial DNA CN was estimated as the ratio of mtDNA to nuDNA coverages.
36566464	4	103	theme	Mitochondrial	543:555	arg1	CN					561:562	Mitochondrial DNA CN	543:562	Mitochondrial DNA CN	543:562	Mitochondrial DNA CN was estimated as the ratio of mtDNA to nuDNA coverages.
36566464	11	104	theme	carcass	1391:1397	arg1	traits					1399:1404	growth and carcass traits	1380:1404	growth and carcass traits	1380:1404	There was no association (P-value >0.10) between mtDNA SNP, haplogroups, or types with growth and carcass traits.
36359764	6	0	theme	HA	893:894	arg1	homeostasis					896:906	HA homeostasis	893:906	HA homeostasis	893:906	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	12	1	theme	neuronal	2105:2112	arg1	pathways					2114:2121	excitatory and inhibitory neuronal pathways	2079:2121	excitatory and inhibitory neuronal pathways underlying peristalsis	2079:2144	TLR2 and TLR4 activation may, however, underlay a provisional benefit on excitatory and inhibitory neuronal pathways underlying peristalsis.
36359764	6	2	from	infiltration	879:890	arg1	intestine					971:979	the small intestine	961:979	the small intestine	961:979	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	5	3	theme	Next	840:843	arg1	Sequencing					856:865	Next Generation Sequencing	840:865	Next Generation Sequencing	840:865	Fecal microbiota composition was evaluated by Next Generation Sequencing.
36359764	3	4	theme	mesenteric	556:565	arg1	ischemia					567:574	METHODS mesenteric ischemia	548:574	METHODS mesenteric ischemia	548:574	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	2	5	theme	small	531:535	arg1	intestine					537:545	the rat small intestine	523:545	the rat small intestine	523:545	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	10	6	theme	non-adrenergic	1718:1731	arg1	relaxations					1733:1743	EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations	1682:1743	EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations	1682:1743	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	10	7	theme	sham-operated	1580:1592	arg1	groups					1594:1599	the non-injured and sham-operated groups	1560:1599	the non-injured and sham-operated groups	1560:1599	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	9	8	theme	muscularis	1454:1463	arg1	propria					1465:1471	the muscularis propria	1450:1471	the muscularis propria	1450:1471	After IRI, HA levels, neutrophil infiltration, and TLR2 and TLR4 expression were significantly enhanced in the muscularis propria, and were significantly reduced to baseline levels by 4-MU.
36359764	10	9	theme	non-injured	1564:1574	arg1	groups					1594:1599	the non-injured and sham-operated groups	1560:1599	the non-injured and sham-operated groups	1560:1599	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	11	10	theme	intestinal	1783:1792	arg1	IRI					1794:1796	intestinal IRI	1783:1796	intestinal IRI	1783:1796	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	6	11	theme	like	917:920	arg1	receptor					922:929	toll like receptor	912:929	toll like receptor (TLR2 and TLR4) expression in the small intestine	912:979	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	12	12	theme	provisional	2056:2066	arg1	benefit					2068:2074	a provisional benefit	2054:2074	a provisional benefit on excitatory and inhibitory neuronal pathways underlying peristalsis	2054:2144	TLR2 and TLR4 activation may, however, underlay a provisional benefit on excitatory and inhibitory neuronal pathways underlying peristalsis.
36359764	11	13	theme	pro-inflammatory	1982:1997	arg1	state					1999:2003	a pro-inflammatory state	1980:2003	a pro-inflammatory state	1980:2003	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	1	14	theme	Intestinal	146:155	arg1	ischemia					157:164	BACKGROUND Intestinal ischemia	135:164	BACKGROUND Intestinal ischemia	135:164	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	2	15	theme	microbiota	490:499	arg1	role					328:331	the pathogenetic role	311:331	the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix,	311:410	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	2	15	theme	microbiota	490:499	arg1	modulator					417:425	a modulator	415:425	a modulator of the enteric neuronal and immune function and of the colonic microbiota	415:499	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	11	16	from	upregulation	1885:1896	arg1	propria					1962:1968	the muscularis propria	1947:1968	the muscularis propria	1947:1968	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	7	17	theme	SsnB	1205:1208	arg1	B					1202:1202	Sparstolonin B	1189:1202	Sparstolonin B (SsnB 10, 30 µM)	1189:1219	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	7	17	theme	SsnB	1205:1208	arg1	µM					1217:1218	SsnB 10, 30 µM	1205:1218	µM	1217:1218	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	5	18	theme	Fecal	794:798	arg1	composition					811:821	Fecal microbiota composition	794:821	Fecal microbiota composition	794:821	Fecal microbiota composition was evaluated by Next Generation Sequencing.
36359764	9	19	theme	baseline	1508:1515	arg1	levels					1517:1522	baseline levels	1508:1522	baseline levels	1508:1522	After IRI, HA levels, neutrophil infiltration, and TLR2 and TLR4 expression were significantly enhanced in the muscularis propria, and were significantly reduced to baseline levels by 4-MU.
36359764	6	20	theme	small	965:969	arg1	intestine					971:979	the small intestine	961:979	the small intestine	961:979	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	4	21	theme	synthesis	734:742	arg1	4-methylumbelliferone					755:775	4-methylumbelliferone	755:775	4-methylumbelliferone (4-MU 25 mg/kg)	755:791	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	4	21	theme	synthesis	734:742	arg1	inhibitor					744:752	the HA synthesis inhibitor	727:752	the HA synthesis inhibitor	727:752	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	0	22	theme	Colonic	81:87	arg1	Microbiota					89:98	Colonic Microbiota	81:98	Colonic Microbiota	81:98	Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury.
36359764	8	23	theme	IRI-induced	1257:1267	arg1	enhancement					1269:1279	IRI-induced enhancement	1257:1279	IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria	1257:1340	RESULTS 4-MU significantly reduced IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria.
36359764	2	24	theme	in	508:509	arg1	IRI					516:518	in vivo IRI	508:518	in vivo IRI in the rat small intestine	508:545	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	2	25	theme	matrix	404:409	arg1	hyaluronan					336:345	hyaluronan	336:345	hyaluronan (HA)	336:350	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	2	25	theme	matrix	404:409	arg1	component					373:381	a glycosaminoglycan component	353:381	a glycosaminoglycan component of the extracellular matrix	353:409	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	7	26	theme	TLR2/4	1171:1176	arg1	inhibitor					1178:1186	the selective TLR2/4 inhibitor	1157:1186	the selective TLR2/4 inhibitor	1157:1186	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	7	26	theme	TLR2/4	1171:1176	arg1	B					1202:1202	Sparstolonin B	1189:1202	Sparstolonin B (SsnB 10, 30 µM)	1189:1219	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	6	27	theme	Western	1060:1066	arg1	approaches					1036:1045	immunohistochemical and biomolecular approaches	999:1045	immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting)	999:1076	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	27	theme	Western	1060:1066	arg1	blotting					1068:1075	Western blotting	1060:1075	Western blotting	1060:1075	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	2	28	theme	neuronal	442:449	arg1	function					462:469	the enteric neuronal and immune function	430:469	the enteric neuronal and immune function	430:469	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	0	29	dep	Intestine	67:75	arg1	the					53:55	the	53:55	the	53:55	Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury.
36359764	0	30	theme	Neuronal	21:28	arg1	Function					41:48	Neuronal and Immune Function	21:48	Neuronal and Immune Function	21:48	Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury.
36359764	6	31	from	expression	947:956	arg1	intestine					971:979	the small intestine	961:979	the small intestine	961:979	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	11	32	theme	target	1911:1916	arg1	receptors					1918:1926	bacterial target receptors	1901:1926	bacterial target receptors	1901:1926	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	11	32	theme	target	1911:1916	arg1	TLR4					1938:1941	TLR4	1938:1941	TLR4	1938:1941	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	11	32	theme	target	1911:1916	arg1	TLR2					1929:1932	TLR2	1929:1932	TLR2	1929:1932	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	10	33	dep	field-stimulated	1631:1646	arg1	Hz					1661:1662	0.1-40 Hz	1654:1662	0.1-40 Hz	1654:1662	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	10	33	dep	field-stimulated	1631:1646	arg1	EFS					1649:1651	EFS	1649:1651	EFS	1649:1651	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	10	34	theme	field-stimulated	1631:1646	arg1	contractions					1665:1676	electrical field-stimulated (EFS, 0.1-40 Hz) contractions	1620:1676	electrical field-stimulated (EFS, 0.1-40 Hz) contractions	1620:1676	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	0	35	theme	Immune	34:39	arg1	Function					41:48	Neuronal and Immune Function	21:48	Neuronal and Immune Function	21:48	Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury.
36359764	8	36	dep	harmful	1296:1302	arg1	bacteria					1333:1340	bacteria	1333:1340	bacteria	1333:1340	RESULTS 4-MU significantly reduced IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria.
36359764	8	36	dep	harmful	1296:1302	arg1	Escherichia					1304:1314	Escherichia	1304:1314	Escherichia	1304:1314	RESULTS 4-MU significantly reduced IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria.
36359764	2	37	theme	immune	455:460	arg1	function					462:469	the enteric neuronal and immune function	430:469	the enteric neuronal and immune function	430:469	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	0	38	theme	Rat	57:59	arg1	Intestine					67:75	Rat Small Intestine	57:75	Rat Small Intestine	57:75	Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury.
36359764	3	39	theme	adult	604:608	arg1	rats					615:618	anesthetized adult male rats	591:618	anesthetized adult male rats	591:618	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	12	40	dep	TLR2	2006:2009	arg1	activation					2020:2029	activation	2020:2029	activation	2020:2029	TLR2 and TLR4 activation may, however, underlay a provisional benefit on excitatory and inhibitory neuronal pathways underlying peristalsis.
36359764	3	41	theme	h	647:647	arg1	reperfusion					649:659	24 h reperfusion	644:659	24 h reperfusion	644:659	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	11	42	theme	bacteria	1813:1820	arg1	overgrowth					1822:1831	harmful bacteria overgrowth	1805:1831	harmful bacteria overgrowth	1805:1831	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	6	43	from	homeostasis	896:906	arg1	intestine					971:979	the small intestine	961:979	the small intestine	961:979	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	9	44	theme	neutrophil	1365:1374	arg1	infiltration					1376:1387	neutrophil infiltration	1365:1387	neutrophil infiltration	1365:1387	After IRI, HA levels, neutrophil infiltration, and TLR2 and TLR4 expression were significantly enhanced in the muscularis propria, and were significantly reduced to baseline levels by 4-MU.
36359764	6	45	theme	toll	912:915	arg1	receptor					922:929	toll like receptor	912:929	toll like receptor (TLR2 and TLR4) expression in the small intestine	912:979	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	11	46	theme	neutrophil	1844:1853	arg1	infiltration					1855:1866	neutrophil infiltration	1844:1866	neutrophil infiltration	1844:1866	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	11	47	theme	bacterial	1901:1909	arg1	receptors					1918:1926	bacterial target receptors	1901:1926	bacterial target receptors	1901:1926	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	11	47	theme	bacterial	1901:1909	arg1	TLR4					1938:1941	TLR4	1938:1941	TLR4	1938:1941	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	11	47	theme	bacterial	1901:1909	arg1	TLR2					1929:1932	TLR2	1929:1932	TLR2	1929:1932	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	10	48	theme	non-cholinergic	1702:1716	arg1	relaxations					1733:1743	EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations	1682:1743	EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations	1682:1743	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	9	49	theme	TLR2	1394:1397	arg1	expression					1408:1417	TLR2 and TLR4 expression	1394:1417	TLR2 and TLR4 expression	1394:1417	After IRI, HA levels, neutrophil infiltration, and TLR2 and TLR4 expression were significantly enhanced in the muscularis propria, and were significantly reduced to baseline levels by 4-MU.
36359764	7	50	theme	Neuromuscular	1079:1091	arg1	responses					1093:1101	Neuromuscular responses	1079:1101	Neuromuscular responses	1079:1101	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	3	51	dep	induced	580:586	arg1	followed					632:639	followed	632:639	followed by 24 h reperfusion	632:659	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	9	52	theme	TLR4	1403:1406	arg1	expression					1408:1417	TLR2 and TLR4 expression	1394:1417	TLR2 and TLR4 expression	1394:1417	After IRI, HA levels, neutrophil infiltration, and TLR2 and TLR4 expression were significantly enhanced in the muscularis propria, and were significantly reduced to baseline levels by 4-MU.
36359764	11	53	theme	muscularis	1951:1960	arg1	propria					1962:1968	the muscularis propria	1947:1968	the muscularis propria	1947:1968	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	6	54	theme	Neutrophil	868:877	arg1	infiltration					879:890	Neutrophil infiltration	868:890	Neutrophil infiltration	868:890	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	12	55	theme	inhibitory	2094:2103	arg1	pathways					2114:2121	excitatory and inhibitory neuronal pathways	2079:2121	excitatory and inhibitory neuronal pathways underlying peristalsis	2079:2144	TLR2 and TLR4 activation may, however, underlay a provisional benefit on excitatory and inhibitory neuronal pathways underlying peristalsis.
36359764	3	56	theme	METHODS	548:554	arg1	ischemia					567:574	METHODS mesenteric ischemia	548:574	METHODS mesenteric ischemia	548:574	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	5	57	theme	Generation	845:854	arg1	Sequencing					856:865	Next Generation Sequencing	840:865	Next Generation Sequencing	840:865	Fecal microbiota composition was evaluated by Next Generation Sequencing.
36359764	2	58	theme	pathogenetic	315:326	arg1	role					328:331	the pathogenetic role	311:331	the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix,	311:410	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	2	58	theme	pathogenetic	315:326	arg1	modulator					417:425	a modulator	415:425	a modulator of the enteric neuronal and immune function and of the colonic microbiota	415:499	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	1	59	theme	acute	203:207	arg1	damage					226:231	acute and long-lasting damage	203:231	acute and long-lasting damage to the neuromuscular compartment and dysmotility	203:280	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	2	60	theme	rat	527:529	arg1	intestine					537:545	the rat small intestine	523:545	the rat small intestine	523:545	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	6	61	dep	receptor	922:929	arg1	TLR4					941:944	TLR4	941:944	TLR4	941:944	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	61	dep	receptor	922:929	arg1	TLR2					932:935	TLR2	932:935	TLR2	932:935	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	8	62	theme	harmful	1296:1302	arg1	enhancement					1269:1279	IRI-induced enhancement	1257:1279	IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria	1257:1340	RESULTS 4-MU significantly reduced IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria.
36359764	11	63	theme	HA	1767:1768	arg1	levels					1770:1775	HA levels	1767:1775	HA levels	1767:1775	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	1	64	theme	long-lasting	213:224	arg1	damage					226:231	acute and long-lasting damage	203:231	acute and long-lasting damage to the neuromuscular compartment and dysmotility	203:280	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	8	65	theme	RESULTS	1222:1228	arg1	4-MU					1230:1233	RESULTS 4-MU	1222:1233	RESULTS 4-MU	1222:1233	RESULTS 4-MU significantly reduced IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria.
36359764	11	66	theme	harmful	1805:1811	arg1	bacteria					1813:1820	harmful bacteria	1805:1820	harmful bacteria overgrowth	1805:1831	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	6	67	theme	receptor	922:929	arg1	expression					947:956	toll like receptor (TLR2 and TLR4) expression	912:956	toll like receptor (TLR2 and TLR4) expression in the small intestine	912:979	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	4	68	theme	sham-operated	671:683	arg1	animals					701:707	Injured, sham-operated and non-injured animals	662:707	Injured, sham-operated and non-injured animals	662:707	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	3	69	theme	male	610:613	arg1	rats					615:618	anesthetized adult male rats	591:618	anesthetized adult male rats	591:618	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	1	70	theme	BACKGROUND	135:144	arg1	ischemia					157:164	BACKGROUND Intestinal ischemia	135:164	BACKGROUND Intestinal ischemia	135:164	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	6	71	theme	immunohistochemical	999:1017	arg1	approaches					1036:1045	immunohistochemical and biomolecular approaches	999:1045	immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting)	999:1076	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	71	theme	immunohistochemical	999:1017	arg1	qRT-PCR					1048:1054	qRT-PCR	1048:1054	qRT-PCR	1048:1054	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	71	theme	immunohistochemical	999:1017	arg1	blotting					1068:1075	Western blotting	1060:1075	Western blotting	1060:1075	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	2	72	from	IRI	516:518	arg1	intestine					537:545	the rat small intestine	523:545	the rat small intestine	523:545	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	8	73	theme	Enterococcus	1320:1331	arg1	enhancement					1269:1279	IRI-induced enhancement	1257:1279	IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria	1257:1340	RESULTS 4-MU significantly reduced IRI-induced enhancement of potentially harmful Escherichia and Enterococcus bacteria.
36359764	4	74	theme	non-injured	689:699	arg1	animals					701:707	Injured, sham-operated and non-injured animals	662:707	Injured, sham-operated and non-injured animals	662:707	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	0	75	theme	Small	61:65	arg1	Intestine					67:75	Rat Small Intestine	57:75	Rat Small Intestine	57:75	Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury.
36359764	3	76	theme	anesthetized	591:602	arg1	rats					615:618	anesthetized adult male rats	591:618	anesthetized adult male rats	591:618	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	5	77	theme	microbiota	800:809	arg1	composition					811:821	Fecal microbiota composition	794:821	Fecal microbiota composition	794:821	Fecal microbiota composition was evaluated by Next Generation Sequencing.
36359764	1	78	theme	ischemia	157:164	arg1	injury					188:193	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury	135:193	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury	135:193	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	1	78	theme	ischemia	157:164	arg1	IRI					183:185	IRI	183:185	IRI	183:185	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	2	79	theme	colonic	482:488	arg1	microbiota					490:499	the colonic microbiota	478:499	the colonic microbiota	478:499	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	7	80	theme	Sparstolonin	1189:1200	arg1	inhibitor					1178:1186	the selective TLR2/4 inhibitor	1157:1186	the selective TLR2/4 inhibitor	1157:1186	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	7	80	theme	Sparstolonin	1189:1200	arg1	B					1202:1202	Sparstolonin B	1189:1202	Sparstolonin B (SsnB 10, 30 µM)	1189:1219	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	7	80	theme	Sparstolonin	1189:1200	arg1	µM					1217:1218	SsnB 10, 30 µM	1205:1218	µM	1217:1218	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	1	81	theme	reperfusion	170:180	arg1	injury					188:193	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury	135:193	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury	135:193	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	1	81	theme	reperfusion	170:180	arg1	IRI					183:185	IRI	183:185	IRI	183:185	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	4	82	theme	Injured	662:668	arg1	animals					701:707	Injured, sham-operated and non-injured animals	662:707	Injured, sham-operated and non-injured animals	662:707	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	7	83	theme	inhibitor	1178:1186	arg1	presence					1145:1152	presence	1145:1152	presence	1145:1152	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	7	83	theme	inhibitor	1178:1186	arg1	absence					1133:1139	absence	1133:1139	absence	1133:1139	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	4	84	theme	HA	731:732	arg1	4-methylumbelliferone					755:775	4-methylumbelliferone	755:775	4-methylumbelliferone (4-MU 25 mg/kg)	755:791	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	4	84	theme	HA	731:732	arg1	inhibitor					744:752	the HA synthesis inhibitor	727:752	the HA synthesis inhibitor	727:752	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	0	85	theme	Ischemic/Reperfusion	106:125	arg1	Injury					127:132	Ischemic/Reperfusion Injury	106:132	Ischemic/Reperfusion Injury	106:132	Hyaluronan Regulates Neuronal and Immune Function in the Rat Small Intestine and Colonic Microbiota after Ischemic/Reperfusion Injury.
36359764	6	86	dep	approaches	1036:1045	arg1	approaches					1036:1045	immunohistochemical and biomolecular approaches	999:1045	immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting)	999:1076	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	86	dep	approaches	1036:1045	arg1	qRT-PCR					1048:1054	qRT-PCR	1048:1054	qRT-PCR	1048:1054	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	86	dep	approaches	1036:1045	arg1	blotting					1068:1075	Western blotting	1060:1075	Western blotting	1060:1075	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	7	87	theme	selective	1161:1169	arg1	inhibitor					1178:1186	the selective TLR2/4 inhibitor	1157:1186	the selective TLR2/4 inhibitor	1157:1186	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	7	87	theme	selective	1161:1169	arg1	B					1202:1202	Sparstolonin B	1189:1202	Sparstolonin B (SsnB 10, 30 µM)	1189:1219	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	2	88	theme	enteric	434:440	arg1	function					462:469	the enteric neuronal and immune function	430:469	the enteric neuronal and immune function	430:469	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	7	89	dep	absence	1133:1139	arg1	the					1129:1131	the	1129:1131	the	1129:1131	Neuromuscular responses were studied in vitro, in the absence and presence of the selective TLR2/4 inhibitor, Sparstolonin B (SsnB 10, 30 µM).
36359764	2	90	dep	in	508:509	arg1	vivo					511:514	vivo	511:514	vivo	511:514	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	12	91	from	benefit	2068:2074	arg1	pathways					2114:2121	excitatory and inhibitory neuronal pathways	2079:2121	excitatory and inhibitory neuronal pathways underlying peristalsis	2079:2144	TLR2 and TLR4 activation may, however, underlay a provisional benefit on excitatory and inhibitory neuronal pathways underlying peristalsis.
36359764	6	92	theme	biomolecular	1023:1034	arg1	approaches					1036:1045	immunohistochemical and biomolecular approaches	999:1045	immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting)	999:1076	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	92	theme	biomolecular	1023:1034	arg1	qRT-PCR					1048:1054	qRT-PCR	1048:1054	qRT-PCR	1048:1054	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	6	92	theme	biomolecular	1023:1034	arg1	blotting					1068:1075	Western blotting	1060:1075	Western blotting	1060:1075	Neutrophil infiltration, HA homeostasis and toll like receptor (TLR2 and TLR4) expression in the small intestine were evaluated by immunohistochemical and biomolecular approaches (qRT-PCR and Western blotting).
36359764	2	93	theme	function	462:469	arg1	role					328:331	the pathogenetic role	311:331	the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix,	311:410	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	2	93	theme	function	462:469	arg1	modulator					417:425	a modulator	415:425	a modulator of the enteric neuronal and immune function and of the colonic microbiota	415:499	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	1	94	theme	neuromuscular	240:252	arg1	compartment					254:264	the neuromuscular compartment	236:264	the neuromuscular compartment	236:264	BACKGROUND Intestinal ischemia and reperfusion (IRI) injury induces acute and long-lasting damage to the neuromuscular compartment and dysmotility.
36359764	11	95	theme	receptors	1918:1926	arg1	upregulation					1885:1896	the upregulation	1881:1896	the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria	1881:1968	CONCLUSIONS enhanced HA levels after intestinal IRI favors harmful bacteria overgrowth, increases neutrophil infiltration and promotes the upregulation of bacterial target receptors, TLR2 and TLR4, in the muscularis propria, inducing a pro-inflammatory state.
36359764	2	96	theme	glycosaminoglycan	355:371	arg1	hyaluronan					336:345	hyaluronan	336:345	hyaluronan (HA)	336:350	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	2	96	theme	glycosaminoglycan	355:371	arg1	component					373:381	a glycosaminoglycan component	353:381	a glycosaminoglycan component of the extracellular matrix	353:409	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	3	97	theme	24	644:645	arg1	h					647:647	h	647:647	h	647:647	METHODS mesenteric ischemia was induced in anesthetized adult male rats for 60 min, followed by 24 h reperfusion.
36359764	9	98	theme	HA	1354:1355	arg1	levels					1357:1362	HA levels	1354:1362	HA levels	1354:1362	After IRI, HA levels, neutrophil infiltration, and TLR2 and TLR4 expression were significantly enhanced in the muscularis propria, and were significantly reduced to baseline levels by 4-MU.
36359764	10	99	dep	EFS-induced	1682:1692	arg1	Hz					1698:1699	10 Hz	1695:1699	10 Hz	1695:1699	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
36359764	2	100	theme	hyaluronan	336:345	arg1	role					328:331	the pathogenetic role	311:331	the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix,	311:410	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	2	100	theme	hyaluronan	336:345	arg1	modulator					417:425	a modulator	415:425	a modulator of the enteric neuronal and immune function and of the colonic microbiota	415:499	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	12	101	theme	excitatory	2079:2088	arg1	pathways					2114:2121	excitatory and inhibitory neuronal pathways	2079:2121	excitatory and inhibitory neuronal pathways underlying peristalsis	2079:2144	TLR2 and TLR4 activation may, however, underlay a provisional benefit on excitatory and inhibitory neuronal pathways underlying peristalsis.
36359764	2	102	theme	extracellular	390:402	arg1	matrix					404:409	the extracellular matrix	386:409	the extracellular matrix	386:409	This study aims to evaluate the pathogenetic role of hyaluronan (HA), a glycosaminoglycan component of the extracellular matrix, as a modulator of the enteric neuronal and immune function and of the colonic microbiota during in vivo IRI in the rat small intestine.
36359764	4	103	theme	4-MU	778:781	arg1	4-methylumbelliferone					755:775	4-methylumbelliferone	755:775	4-methylumbelliferone (4-MU 25 mg/kg)	755:791	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	4	103	theme	4-MU	778:781	arg1	mg/kg					786:790	4-MU 25 mg/kg	778:790	4-MU 25 mg/kg	778:790	Injured, sham-operated and non-injured animals were treated with the HA synthesis inhibitor, 4-methylumbelliferone (4-MU 25 mg/kg).
36359764	10	104	theme	EFS-induced	1682:1692	arg1	relaxations					1733:1743	EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations	1682:1743	EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations	1682:1743	In the injured, but not in the non-injured and sham-operated groups, SsnB reduced both electrical field-stimulated (EFS, 0.1-40 Hz) contractions and EFS-induced (10 Hz) non-cholinergic non-adrenergic relaxations.
35932606	9	0	theme	regional	1654:1661	arg1	outliers					1663:1670	three regional outliers	1648:1670	three regional outliers	1648:1670	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	11	1	theme	interleukin	1888:1898	arg1	IL					1901:1902	interleukin (IL)-10	1888:1906	interleukin (IL)-10	1888:1906	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	1	theme	interleukin	1888:1898	arg1	occludens-1					1858:1868	zonula occludens-1	1851:1868	zonula occludens-1 (ZO-1)	1851:1875	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	2	theme	epidermal	1939:1947	arg1	EGFR					1973:1976	EGFR	1973:1976	EGFR	1973:1976	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	2	theme	epidermal	1939:1947	arg1	receptor					1963:1970	epidermal growth factor receptor	1939:1970	epidermal growth factor receptor (EGFR)	1939:1977	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	9	3	theme	HCD	1590:1592	arg1	samples					1594:1600	RESULTS HCD samples	1582:1600	RESULTS HCD samples	1582:1600	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	7	4	theme	Caco-2	1346:1351	arg1	monolayers					1353:1362	tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers	1304:1362	tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers	1304:1362	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35932606	6	5	theme	Chromatography-Mass	1160:1178	arg1	HPLC-MS					1194:1200	HPLC-MS	1194:1200	HPLC-MS	1194:1200	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	6	5	theme	Chromatography-Mass	1160:1178	arg1	Spectrometry					1180:1191	High-Performance Liquid Chromatography-Mass Spectrometry	1136:1191	High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)	1136:1201	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	11	6	theme	signal-regulated	2055:2070	arg1	kinase					2072:2077	EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2	1986:2081	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	6	theme	signal-regulated	2055:2070	arg1	ERK					2084:2086	ERK 1/2	2084:2090	ERK 1/2	2084:2090	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	13	7	theme	barrier	2376:2382	arg1	function					2384:2391	intestinal barrier function	2365:2391	intestinal barrier function	2365:2391	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	3	8	theme	HCD	695:697	arg1	action					685:690	action	685:690	action of HCD	685:697	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	6	9	theme	High-Performance	1136:1151	arg1	HPLC-MS					1194:1200	HPLC-MS	1194:1200	HPLC-MS	1194:1200	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	6	9	theme	High-Performance	1136:1151	arg1	Spectrometry					1180:1191	High-Performance Liquid Chromatography-Mass Spectrometry	1136:1191	High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)	1136:1201	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	1	10	theme	aqueous	195:201	arg1	extract					203:209	A traditionally prepared aqueous extract	170:209	A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC)	170:270	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	1	10	theme	aqueous	195:201	arg1	decoction					214:222	= decoction	212:222	= decoction	212:222	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	14	11	theme	"	2538:2538	arg1	material					2548:2555	the "Daodi" quality material	2528:2555	the "Daodi" quality material	2528:2555	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	9	12	theme	chemical	1622:1629	arg1	fingerprints					1631:1642	different chemical fingerprints	1612:1642	different chemical fingerprints	1612:1642	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	3	13	theme	action	685:690	arg1	mechanism					672:680	the mechanism	668:680	the mechanism of action of HCD	668:697	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	0	14	theme	MAPK	125:128	arg1	pathway					150:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	11	15	theme	factor	1956:1961	arg1	EGFR					1973:1976	EGFR	1973:1976	EGFR	1973:1976	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	15	theme	factor	1956:1961	arg1	receptor					1963:1970	epidermal growth factor receptor	1939:1970	epidermal growth factor receptor (EGFR)	1939:1977	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	0	16	theme	ERK1/2	131:136	arg1	pathway					150:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	11	17	theme	protein	2019:2025	arg1	kinase					2072:2077	EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2	1986:2081	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	17	theme	protein	2019:2025	arg1	ERK					2084:2086	ERK 1/2	2084:2090	ERK 1/2	2084:2090	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	0	18	theme	signalling	139:148	arg1	pathway					150:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	11	19	theme	EGFR-dependent	1986:1999	arg1	kinase					2072:2077	EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2	1986:2081	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	19	theme	EGFR-dependent	1986:1999	arg1	ERK					2084:2086	ERK 1/2	2084:2090	ERK 1/2	2084:2090	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	14	20	theme	"	2429:2429	arg1	product					2431:2437	a "Daodi" product	2421:2437	a "Daodi" product	2421:2437	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	12	21	theme	colonic	2229:2235	arg1	tissues					2237:2243	colonic tissues	2229:2243	colonic tissues	2229:2243	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	6	22	theme	Nuclear	1099:1105	arg1	NMR					1127:1129	NMR	1127:1129	NMR	1127:1129	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	6	22	theme	Nuclear	1099:1105	arg1	Resonance					1116:1124	Nuclear Magnetic Resonance	1099:1124	Nuclear Magnetic Resonance (NMR)	1099:1130	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	3	23	theme	chemical	720:727	arg1	variation					729:737	chemical variation	720:737	chemical variation	720:737	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	3	24	theme	chemical	567:574	arg1	composition					576:586	the chemical composition	563:586	the chemical composition of HC aqueous decoction (HCD)	563:616	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	3	25	dep	PURPOSE	516:522	arg1	aimed					534:538	aimed	534:538	aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity	534:780	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	9	26	theme	Component	1701:1709	arg1	PCA					1721:1723	PCA	1721:1723	PCA	1721:1723	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	9	26	theme	Component	1701:1709	arg1	Analysis					1711:1718	Principal Component Analysis	1691:1718	Principal Component Analysis (PCA)	1691:1724	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	2	27	from	studies	389:395	arg1	HC					400:401	HC	400:401	HC	400:401	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	0	28	theme	barrier	86:92	arg1	function					94:101	intestinal barrier function	75:101	intestinal barrier function	75:101	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	1	29	theme	Chinese	302:308	arg1	TCM					320:322	TCM	320:322	TCM	320:322	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	1	29	theme	Chinese	302:308	arg1	Medicine					310:317	Traditional Chinese Medicine	290:317	Traditional Chinese Medicine (TCM)	290:323	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	11	30	theme	MAPK	2035:2038	arg1	kinase					2072:2077	EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2	1986:2081	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	30	theme	MAPK	2035:2038	arg1	ERK					2084:2086	ERK 1/2	2084:2090	ERK 1/2	2084:2090	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	3	31	theme	HCDs	750:753	arg1	activity					773:780	HCDs anti-inflammatory activity	750:780	HCDs anti-inflammatory activity	750:780	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	5	32	theme	Control	1006:1012	arg1	sample					1019:1024	A Quality Control (QC) sample	996:1024	A Quality Control (QC) sample	996:1024	A Quality Control (QC) sample was prepared by combining individual HCD extracts.
35932606	14	33	theme	biological	2459:2468	arg1	activity					2470:2477	the greatest biological activity	2446:2477	the greatest biological activity	2446:2477	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	14	34	theme	inflammation	2628:2639	arg1	treatment					2615:2623	the treatment	2611:2623	the treatment of inflammation	2611:2639	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	4	35	theme	traditional	932:942	arg1	method					954:959	the traditional decoction method	928:959	the traditional decoction method to yield their corresponding HCDs	928:993	METHOD Sixteen samples of HC were purchased from Sichuan, Hubei and Anhui provinces in the People's Republic of China (PRC) and were prepared by the traditional decoction method to yield their corresponding HCDs.
35932606	11	36	theme	zonula	1851:1856	arg1	IL					1901:1902	interleukin (IL)-10	1888:1906	interleukin (IL)-10	1888:1906	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	36	theme	zonula	1851:1856	arg1	ZO-1					1871:1874	ZO-1	1871:1874	ZO-1	1871:1874	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	36	theme	zonula	1851:1856	arg1	occludin					1878:1885	occludin	1878:1885	occludin	1878:1885	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	36	theme	zonula	1851:1856	arg1	occludens-1					1858:1868	zonula occludens-1	1851:1868	zonula occludens-1 (ZO-1)	1851:1875	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	7	37	theme	intestinal	1253:1262	arg1	function					1272:1279	intestinal barrier function	1253:1279	intestinal barrier function of HCD	1253:1286	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35932606	2	38	theme	biological	378:387	arg1	studies					389:395	Previous chemical and biological studies	356:395	Previous chemical and biological studies on HC	356:401	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	0	39	theme	Chinese	16:22	arg1	Medicine					24:31	The Traditional Chinese Medicine	0:31	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	12	40	dep	in	2183:2184	arg1	vivo					2186:2189	vivo	2186:2189	vivo	2186:2189	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	14	41	theme	greatest	2450:2457	arg1	activity					2470:2477	the greatest biological activity	2446:2477	the greatest biological activity	2446:2477	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	13	42	theme	different	2333:2341	arg1	levels					2343:2348	different levels	2333:2348	different levels of activity on intestinal barrier function and inflammation	2333:2408	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	2	43	theme	organic	426:432	arg1	extracts					434:441	organic extracts	426:441	organic extracts rather than the aqueous decoction, which is the traditional formulation	426:513	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	2	44	theme	chemical	365:372	arg1	studies					389:395	Previous chemical and biological studies	356:395	Previous chemical and biological studies on HC	356:401	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	0	45	theme	Houttuynia	33:42	arg1	decoction					58:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	7	46	theme	anti-inflammatory	1208:1224	arg1	activities					1226:1235	The anti-inflammatory activities	1204:1235	The anti-inflammatory activities associated with intestinal barrier function of HCD	1204:1286	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35932606	3	47	theme	aqueous	594:600	arg1	decoction					602:610	HC aqueous decoction	591:610	HC aqueous decoction (HCD)	591:616	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	3	47	theme	aqueous	594:600	arg1	HCD					613:615	HCD	613:615	HCD	613:615	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	12	48	theme	beneficial	2117:2126	arg1	effect					2128:2133	This beneficial effect	2112:2133	This beneficial effect on intestinal inflammation	2112:2160	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	11	49	theme	kinase	2072:2077	arg1	pathway					2103:2109	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	0	50	theme	Thunb	52:56	arg1	decoction					58:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	5	51	theme	HCD	1063:1065	arg1	extracts					1067:1074	individual HCD extracts	1052:1074	individual HCD extracts	1052:1074	A Quality Control (QC) sample was prepared by combining individual HCD extracts.
35932606	7	52	theme	-induced	1419:1426	arg1	colitis					1435:1441	Dextran Sulfate Sodium (DSS)-induced murine colitis	1391:1441	Dextran Sulfate Sodium (DSS)-induced murine colitis	1391:1441	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35932606	8	53	theme	disease	1492:1498	arg1	scores					1509:1514	disease severity scores	1492:1514	disease severity scores	1492:1514	Proteins involved in inflammation, mRNA levels, disease severity scores, and histology involved in intestinal inflammation were analysed.
35932606	1	54	used	used	282:285	arg2	decoction					214:222	= decoction	212:222	= decoction	212:222	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	1	54	used	used	282:285	arg2	extract					203:209	A traditionally prepared aqueous extract	170:209	A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC)	170:270	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	13	55	theme	test	2283:2286	arg1	HCDs					2288:2291	the test HCDs	2279:2291	the test HCDs	2279:2291	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	13	55	theme	test	2283:2286	arg1	different					2309:2317	different	2309:2317	different	2309:2317	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	11	56	theme	anti-inflammatory	1813:1829	arg1	activity					1831:1838	vitro anti-inflammatory activity	1807:1838	vitro anti-inflammatory activity	1807:1838	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	1	57	theme	Thunb	247:251	arg1	extract					203:209	A traditionally prepared aqueous extract	170:209	A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC)	170:270	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	1	57	theme	Thunb	247:251	arg1	decoction					214:222	= decoction	212:222	= decoction	212:222	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	4	58	theme	HC	809:810	arg1	samples					798:804	METHOD Sixteen samples	783:804	METHOD Sixteen samples of HC	783:810	METHOD Sixteen samples of HC were purchased from Sichuan, Hubei and Anhui provinces in the People's Republic of China (PRC) and were prepared by the traditional decoction method to yield their corresponding HCDs.
35932606	14	59	theme	material	2548:2555	arg1	importance					2514:2523	the importance	2510:2523	the importance of the "Daodi" quality material in TCM	2510:2562	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	4	60	theme	corresponding	976:988	arg1	HCDs					990:993	their corresponding HCDs	970:993	their corresponding HCDs	970:993	METHOD Sixteen samples of HC were purchased from Sichuan, Hubei and Anhui provinces in the People's Republic of China (PRC) and were prepared by the traditional decoction method to yield their corresponding HCDs.
35932606	11	61	theme	extracellular	2041:2053	arg1	kinase					2072:2077	EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2	1986:2081	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	61	theme	extracellular	2041:2053	arg1	ERK					2084:2086	ERK 1/2	2084:2090	ERK 1/2	2084:2090	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	9	62	theme	RESULTS	1582:1588	arg1	samples					1594:1600	RESULTS HCD samples	1582:1600	RESULTS HCD samples	1582:1600	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	8	63	theme	intestinal	1543:1552	arg1	inflammation					1554:1565	intestinal inflammation	1543:1565	intestinal inflammation	1543:1565	Proteins involved in inflammation, mRNA levels, disease severity scores, and histology involved in intestinal inflammation were analysed.
35932606	12	64	from	level	2220:2224	arg1	tissues					2237:2243	colonic tissues	2229:2243	colonic tissues	2229:2243	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	4	65	theme	METHOD	783:788	arg1	samples					798:804	METHOD Sixteen samples	783:804	METHOD Sixteen samples of HC	783:810	METHOD Sixteen samples of HC were purchased from Sichuan, Hubei and Anhui provinces in the People's Republic of China (PRC) and were prepared by the traditional decoction method to yield their corresponding HCDs.
35932606	4	66	dep	Sichuan	832:838	arg1	provinces					857:865	provinces	857:865	provinces	857:865	METHOD Sixteen samples of HC were purchased from Sichuan, Hubei and Anhui provinces in the People's Republic of China (PRC) and were prepared by the traditional decoction method to yield their corresponding HCDs.
35932606	6	67	theme	Liquid	1153:1158	arg1	HPLC-MS					1194:1200	HPLC-MS	1194:1200	HPLC-MS	1194:1200	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	6	67	theme	Liquid	1153:1158	arg1	Spectrometry					1180:1191	High-Performance Liquid Chromatography-Mass Spectrometry	1136:1191	High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)	1136:1201	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	1	68	theme	prepared	186:193	arg1	extract					203:209	A traditionally prepared aqueous extract	170:209	A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC)	170:270	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	1	68	theme	prepared	186:193	arg1	decoction					214:222	= decoction	212:222	= decoction	212:222	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	14	69	theme	quality	2540:2546	arg1	material					2548:2555	the "Daodi" quality material	2528:2555	the "Daodi" quality material	2528:2555	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	9	70	theme	different	1612:1620	arg1	fingerprints					1631:1642	different chemical fingerprints	1612:1642	different chemical fingerprints	1612:1642	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	7	71	theme	activated	1336:1344	arg1	monolayers					1353:1362	tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers	1304:1362	tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers	1304:1362	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35932606	13	72	dep	CONCLUSION	2246:2255	arg1	shows					2268:2272	shows	2268:2272	shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation	2268:2408	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	11	73	theme	growth	1949:1954	arg1	EGFR					1973:1976	EGFR	1973:1976	EGFR	1973:1976	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	73	theme	growth	1949:1954	arg1	receptor					1963:1970	epidermal growth factor receptor	1939:1970	epidermal growth factor receptor (EGFR)	1939:1977	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	14	74	theme	Daodi	2533:2537	arg1	material					2548:2555	the "Daodi" quality material	2528:2555	the "Daodi" quality material	2528:2555	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	2	75	theme	traditional	491:501	arg1	decoction					467:475	the aqueous decoction	455:475	the aqueous decoction	455:475	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	2	75	theme	traditional	491:501	arg1	formulation					503:513	the traditional formulation	487:513	the traditional formulation	487:513	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	14	76	from	importance	2514:2523	arg1	TCM					2560:2562	TCM	2560:2562	TCM	2560:2562	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	1	77	theme	inflammatory	334:345	arg1	disease					347:353	inflammatory disease	334:353	inflammatory disease	334:353	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	12	78	from	effect	2128:2133	arg1	inflammation					2149:2160	intestinal inflammation	2138:2160	intestinal inflammation	2138:2160	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	1	79	theme	=	212:212	arg1	extract					203:209	A traditionally prepared aqueous extract	170:209	A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC)	170:270	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	1	79	theme	=	212:212	arg1	decoction					214:222	= decoction	212:222	= decoction	212:222	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	13	80	theme	intestinal	2365:2374	arg1	function					2384:2391	intestinal barrier function	2365:2391	intestinal barrier function	2365:2391	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	5	81	theme	Quality	998:1004	arg1	QC					1015:1016	QC	1015:1016	QC	1015:1016	A Quality Control (QC) sample was prepared by combining individual HCD extracts.
35932606	5	81	theme	Quality	998:1004	arg1	Control					1006:1012	Quality Control	998:1012	A Quality Control (QC) sample	996:1024	A Quality Control (QC) sample was prepared by combining individual HCD extracts.
35932606	10	82	theme	phytochemical	1735:1747	arg1	metabolites					1749:1759	Fifteen phytochemical metabolites	1727:1759	Fifteen phytochemical metabolites	1727:1759	Fifteen phytochemical metabolites were identified and quantified.
35932606	11	83	theme	kinase	2027:2032	arg1	kinase					2072:2077	EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2	1986:2081	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	83	theme	kinase	2027:2032	arg1	ERK					2084:2086	ERK 1/2	2084:2090	ERK 1/2	2084:2090	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	14	84	dep	showed	2439:2444	arg1	supporting					2568:2577	supporting	2568:2577	supporting the traditional used of HCD for the treatment of inflammation	2568:2639	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	14	84	dep	showed	2439:2444	arg1	validating					2499:2508	validating	2499:2508	validating the importance of the "Daodi" quality material in TCM	2499:2562	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	14	85	theme	Daodi	2424:2428	arg1	product					2431:2437	a "Daodi" product	2421:2437	a "Daodi" product	2421:2437	Moreover, a "Daodi" product showed the greatest biological activity in this study, thus validating the importance of the "Daodi" quality material in TCM and supporting the traditional used of HCD for the treatment of inflammation.
35932606	11	86	theme	mitogen-activated	2001:2017	arg1	kinase					2072:2077	EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2	1986:2081	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	11	86	theme	mitogen-activated	2001:2017	arg1	ERK					2084:2086	ERK 1/2	2084:2090	ERK 1/2	2084:2090	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	6	87	theme	Magnetic	1107:1114	arg1	NMR					1127:1129	NMR	1127:1129	NMR	1127:1129	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	6	87	theme	Magnetic	1107:1114	arg1	Resonance					1116:1124	Nuclear Magnetic Resonance	1099:1124	Nuclear Magnetic Resonance (NMR)	1099:1130	HCDs were analysed by Nuclear Magnetic Resonance (NMR) and High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS).
35932606	0	88	theme	intestinal	75:84	arg1	function					94:101	intestinal barrier function	75:101	intestinal barrier function	75:101	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	9	89	theme	Principal	1691:1699	arg1	PCA					1721:1723	PCA	1721:1723	PCA	1721:1723	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	9	89	theme	Principal	1691:1699	arg1	Analysis					1711:1718	Principal Component Analysis	1691:1718	Principal Component Analysis (PCA)	1691:1724	RESULTS HCD samples exhibited different chemical fingerprints and three regional outliers were identified by Principal Component Analysis (PCA).
35932606	7	90	theme	HCD	1284:1286	arg1	function					1272:1279	intestinal barrier function	1253:1279	intestinal barrier function of HCD	1253:1286	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35932606	1	91	theme	Traditional	290:300	arg1	TCM					320:322	TCM	320:322	TCM	320:322	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	1	91	theme	Traditional	290:300	arg1	Medicine					310:317	Traditional Chinese Medicine	290:317	Traditional Chinese Medicine (TCM)	290:323	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	12	92	theme	molecular	2210:2218	arg1	level					2220:2224	a molecular level	2208:2224	a molecular level in colonic tissues	2208:2243	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	13	93	from	levels	2343:2348	arg1	function					2384:2391	intestinal barrier function	2365:2391	intestinal barrier function	2365:2391	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	13	93	from	levels	2343:2348	arg1	inflammation					2397:2408	inflammation	2397:2408	inflammation	2397:2408	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	12	94	theme	in	2183:2184	arg1	model					2199:2203	the in vivo colitis model	2179:2203	the in vivo colitis model	2179:2203	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	3	95	theme	anti-inflammatory	755:771	arg1	activity					773:780	HCDs anti-inflammatory activity	750:780	HCDs anti-inflammatory activity	750:780	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	0	96	theme	dependent	115:123	arg1	pathway					150:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	an EGFR dependent MAPK (ERK1/2) signalling pathway	107:156	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	13	97	theme	activity	2353:2360	arg1	levels					2343:2348	different levels	2333:2348	different levels of activity on intestinal barrier function and inflammation	2333:2408	CONCLUSION This study shows that the test HCDs were chemically different, resulting in different levels of activity on intestinal barrier function and inflammation.
35932606	7	98	theme	barrier	1264:1270	arg1	function					1272:1279	intestinal barrier function	1253:1279	intestinal barrier function of HCD	1253:1286	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35932606	2	99	theme	aqueous	459:465	arg1	decoction					467:475	the aqueous decoction	455:475	the aqueous decoction	455:475	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	2	99	theme	aqueous	459:465	arg1	formulation					503:513	the traditional formulation	487:513	the traditional formulation	487:513	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	12	100	theme	colitis	2191:2197	arg1	model					2199:2203	the in vivo colitis model	2179:2203	the in vivo colitis model	2179:2203	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	0	101	theme	Traditional	4:14	arg1	Medicine					24:31	The Traditional Chinese Medicine	0:31	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	11	102	theme	signaling	2093:2101	arg1	pathway					2103:2109	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway	1983:2109	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	1	103	dep	BACKGROUND	159:168	arg1	used					282:285	used	282:285	is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease	272:353	BACKGROUND A traditionally prepared aqueous extract (= decoction) of Houttuynia cordata Thunb (Yu xing cao) (HC) is widely used in Traditional Chinese Medicine (TCM) to treat inflammatory disease.
35932606	0	104	theme	Medicine	24:31	arg1	decoction					58:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	4	105	theme	decoction	944:952	arg1	method					954:959	the traditional decoction method	928:959	the traditional decoction method to yield their corresponding HCDs	928:993	METHOD Sixteen samples of HC were purchased from Sichuan, Hubei and Anhui provinces in the People's Republic of China (PRC) and were prepared by the traditional decoction method to yield their corresponding HCDs.
35932606	8	106	theme	mRNA	1479:1482	arg1	levels					1484:1489	mRNA levels	1479:1489	mRNA levels	1479:1489	Proteins involved in inflammation, mRNA levels, disease severity scores, and histology involved in intestinal inflammation were analysed.
35932606	3	107	theme	decoction	602:610	arg1	composition					576:586	the chemical composition	563:586	the chemical composition of HC aqueous decoction (HCD)	563:616	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	12	108	theme	intestinal	2138:2147	arg1	inflammation					2149:2160	intestinal inflammation	2138:2160	intestinal inflammation	2138:2160	This beneficial effect on intestinal inflammation was also seen in the in vivo colitis model at a molecular level in colonic tissues.
35932606	0	109	theme	cordata	44:50	arg1	decoction					58:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction	0:66	The Traditional Chinese Medicine Houttuynia cordata Thunb decoction alters intestinal barrier function via an EGFR dependent MAPK (ERK1/2) signalling pathway.
35932606	3	110	theme	HC	591:592	arg1	decoction					602:610	HC aqueous decoction	591:610	HC aqueous decoction (HCD)	591:616	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	3	110	theme	HC	591:592	arg1	HCD					613:615	HCD	613:615	HCD	613:615	PURPOSE The study aimed to investigate whether the chemical composition of HC aqueous decoction (HCD) varies with geographical sourcing, to investigate the mechanism of action of HCD, and to determine if chemical variation impacts on HCDs anti-inflammatory activity.
35932606	2	111	theme	Previous	356:363	arg1	studies					389:395	Previous chemical and biological studies	356:395	Previous chemical and biological studies on HC	356:401	Previous chemical and biological studies on HC have mainly focused on organic extracts rather than the aqueous decoction, which is the traditional formulation.
35932606	5	112	theme	individual	1052:1061	arg1	extracts					1067:1074	individual HCD extracts	1052:1074	individual HCD extracts	1052:1074	A Quality Control (QC) sample was prepared by combining individual HCD extracts.
35932606	8	113	theme	severity	1500:1507	arg1	scores					1509:1514	disease severity scores	1492:1514	disease severity scores	1492:1514	Proteins involved in inflammation, mRNA levels, disease severity scores, and histology involved in intestinal inflammation were analysed.
35932606	4	114	theme	China	895:899	arg1	Republic					883:890	the People's Republic	870:890	the People's Republic of China (PRC)	870:905	METHOD Sixteen samples of HC were purchased from Sichuan, Hubei and Anhui provinces in the People's Republic of China (PRC) and were prepared by the traditional decoction method to yield their corresponding HCDs.
35932606	11	115	theme	vitro	1807:1811	arg1	activity					1831:1838	vitro anti-inflammatory activity	1807:1838	vitro anti-inflammatory activity	1807:1838	HCD showed in vitro anti-inflammatory activity, enhancing zonula occludens-1 (ZO-1), occludin, interleukin (IL)-10 and decreasing IL-1β, IL-6 and epidermal growth factor receptor (EGFR) via an EGFR-dependent mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase 1/2 (ERK 1/2) signaling pathway.
35932606	7	116	theme	murine	1428:1433	arg1	colitis					1435:1441	Dextran Sulfate Sodium (DSS)-induced murine colitis	1391:1441	Dextran Sulfate Sodium (DSS)-induced murine colitis	1391:1441	The anti-inflammatory activities associated with intestinal barrier function of HCD were studied by tumor necrosis factor-α (TNF-α) activated Caco-2 monolayers in vitro and in vivo using Dextran Sulfate Sodium (DSS)-induced murine colitis.
35930073	5	0	from	set	780:782	arg1	tract					745:749	the digestive tract	731:749	the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	731:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	0	from	set	780:782	arg1	nectar					720:725	floral nectar	713:725	floral nectar	713:725	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	1	theme	Acinetobacter	639:651	arg1	isolates					690:697	Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates	639:697	Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	639:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	2	theme	%	850:850	arg1	w/v					852:854	15% w/v or 50% w/v	837:854	w/v	852:854	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	3	theme	glucose + 1/2	1026:1038	arg1	fructose					1040:1047	1/2 glucose + 1/2 fructose	1022:1047	1/2 glucose + 1/2 fructose	1022:1047	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	4	theme	flower-visiting	754:768	arg1	bees					770:773	flower-visiting bees	754:773	flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	754:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	4	5	theme	bacteria	501:508	arg1	response					483:490	the response	479:490	the response of these bacteria to variation in the chemical characteristics of floral nectar	479:570	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	3	6	theme	microorganisms	217:230	arg1	microorganisms					217:230	microorganisms	217:230	microorganisms that depend predominantly on animal visitors for their dispersal	217:295	The floral nectar of angiosperms harbors a variety of microorganisms that depend predominantly on animal visitors for their dispersal.
35930073	3	6	theme	microorganisms	217:230	arg1	variety					206:212	a variety	204:212	a variety of microorganisms that depend predominantly on animal visitors for their dispersal	204:295	The floral nectar of angiosperms harbors a variety of microorganisms that depend predominantly on animal visitors for their dispersal.
35930073	8	7	from	deeper	1468:1473	arg1	phylogeny					1478:1486	phylogeny	1478:1486	phylogeny	1478:1486	Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny.
35930073	4	8	theme	nectar	565:570	arg1	characteristics					539:553	the chemical characteristics	526:553	the chemical characteristics of floral nectar	526:570	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	0	9	theme	Acinetobacter	94:106	arg1	spp					108:110	Acinetobacter spp	94:110	Acinetobacter spp	94:110	Sugar Concentration, Nitrogen Availability, and Phylogenetic Factors Determine the Ability of Acinetobacter spp.
35930073	9	10	theme	Acinetobacter	1544:1556	arg1	spp					1558:1560	Acinetobacter spp	1544:1560	Acinetobacter spp	1544:1560	Overall, these results demonstrate that the ability of Acinetobacter spp.
35930073	3	11	theme	floral	167:172	arg1	nectar					174:179	The floral nectar	163:179	The floral nectar of angiosperms	163:194	The floral nectar of angiosperms harbors a variety of microorganisms that depend predominantly on animal visitors for their dispersal.
35930073	8	12	theme	phylogenetic	1260:1271	arg1	analyses					1273:1280	phylogenetic analyses	1260:1280	phylogenetic analyses	1260:1280	Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny.
35930073	7	13	theme	sugar	1140:1144	arg1	w/v					1165:1167	50% w/v	1161:1167	50% w/v	1161:1167	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	13	theme	sugar	1140:1144	arg1	concentration					1146:1158	elevated sugar concentration	1131:1158	elevated sugar concentration (50% w/v)	1131:1168	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	2	14	theme	Floral	148:153	arg1	Nectar					155:160	Floral Nectar	148:160	Floral Nectar	148:160	to Grow in Floral Nectar.
35930073	5	15	dep	content	867:873	arg1	348/167 ppm					893:903	348/167 ppm	893:903	348/167 ppm	893:903	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	15	dep	content	867:873	arg1	3.48/1.67 ppm					876:888	3.48/1.67 ppm	876:888	3.48/1.67 ppm	876:888	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	16	theme	total	908:912	arg1	nitrogen					929:936	total nitrogen/amino nitrogen	908:936	total nitrogen/amino nitrogen	908:936	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	4	17	theme	limited	453:459	arg1	information					461:471	limited information	453:471	limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar	453:570	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	5	18	theme	nitrogen/amino	914:927	arg1	nitrogen					929:936	total nitrogen/amino nitrogen	908:936	total nitrogen/amino nitrogen	908:936	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	19	dep	composition	950:960	arg1	fructose					1040:1047	1/2 glucose + 1/2 fructose	1022:1047	1/2 glucose + 1/2 fructose	1022:1047	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	19	dep	composition	950:960	arg1	fructose					1009:1016	1/3 sucrose + 1/3 glucose + 1/3 fructose	977:1016	1/3 sucrose + 1/3 glucose + 1/3 fructose	977:1016	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	19	dep	composition	950:960	arg1	sucrose					968:974	only sucrose	963:974	only sucrose	963:974	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	0	20	theme	spp	108:110	arg1	Ability					83:89	the Ability	79:89	the Ability of Acinetobacter spp	79:110	Sugar Concentration, Nitrogen Availability, and Phylogenetic Factors Determine the Ability of Acinetobacter spp.
35930073	5	21	theme	diverse	617:623	arg1	collection					625:634	a diverse collection	615:634	a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	615:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	3	22	theme	angiosperms	184:194	arg1	nectar					174:179	The floral nectar	163:179	The floral nectar of angiosperms	163:194	The floral nectar of angiosperms harbors a variety of microorganisms that depend predominantly on animal visitors for their dispersal.
35930073	5	23	theme	nitrogen	929:936	arg1	348/167 ppm					893:903	348/167 ppm	893:903	348/167 ppm	893:903	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	23	theme	nitrogen	929:936	arg1	3.48/1.67 ppm					876:888	3.48/1.67 ppm	876:888	3.48/1.67 ppm	876:888	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	11	24	theme	bacterial	1652:1660	arg1	phylogeny					1662:1670	bacterial phylogeny	1652:1670	bacterial phylogeny	1652:1670	to grow in floral nectar largely depends on nectar chemistry and bacterial phylogeny.
35930073	5	25	theme	collection	625:634	arg1	performance					600:610	the growth performance	589:610	the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	589:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	4	26	theme	known	365:369	arg1	species					371:377	all currently known species	351:377	all currently known species of Rosenbergiella	351:395	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	7	27	theme	elevated	1131:1138	arg1	w/v					1165:1167	50% w/v	1161:1167	50% w/v	1161:1167	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	27	theme	elevated	1131:1138	arg1	concentration					1146:1158	elevated sugar concentration	1131:1158	elevated sugar concentration (50% w/v)	1131:1168	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	11	28	theme	floral	1598:1603	arg1	nectar					1605:1610	floral nectar	1598:1610	floral nectar	1598:1610	to grow in floral nectar largely depends on nectar chemistry and bacterial phylogeny.
35930073	5	29	theme	%	839:839	arg1	w/v					841:843	15% w/v or 50% w/v	837:854	w/v	841:843	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	0	30	theme	Sugar	0:4	arg1	Concentration					6:18	Sugar Concentration	0:18	Sugar Concentration	0:18	Sugar Concentration, Nitrogen Availability, and Phylogenetic Factors Determine the Ability of Acinetobacter spp.
35930073	5	31	from	tract	745:749	arg1	set					780:782	a set	778:782	a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	778:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	31	from	tract	745:749	arg1	nectars					801:807	12 artificial nectars	787:807	12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	787:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	4	32	theme	Rosenbergiella	382:395	arg1	members					312:318	some members	307:318	some members of the genus Acinetobacter	307:345	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	4	32	theme	Rosenbergiella	382:395	arg1	species					371:377	all currently known species	351:377	all currently known species of Rosenbergiella	351:395	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	7	33	theme	%	1163:1163	arg1	w/v					1165:1167	50% w/v	1161:1167	50% w/v	1161:1167	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	33	theme	%	1163:1163	arg1	concentration					1146:1158	elevated sugar concentration	1131:1158	elevated sugar concentration (50% w/v)	1131:1168	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	8	34	from	phylogeny	1478:1486	arg1	deeper					1468:1473	deeper	1468:1473	deeper	1468:1473	Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny.
35930073	0	35	theme	Nitrogen	21:28	arg1	Availability					30:41	Nitrogen Availability	21:41	Nitrogen Availability	21:41	Sugar Concentration, Nitrogen Availability, and Phylogenetic Factors Determine the Ability of Acinetobacter spp.
35930073	4	36	theme	chemical	530:537	arg1	characteristics					539:553	the chemical characteristics	526:553	the chemical characteristics of floral nectar	526:570	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	5	37	theme	nectars	801:807	arg1	set					780:782	a set	778:782	a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	778:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	37	theme	nectars	801:807	arg1	nectars					801:807	12 artificial nectars	787:807	12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	787:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	4	38	theme	floral	558:563	arg1	nectar					565:570	floral nectar	558:570	floral nectar	558:570	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	5	39	theme	isolates	690:697	arg1	collection					625:634	a diverse collection	615:634	a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	615:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	8	40	theme	related	1397:1403	arg1	isolates					1405:1412	closely related isolates	1389:1412	closely related isolates	1389:1412	Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny.
35930073	7	41	theme	bacterial	1229:1237	arg1	growth					1239:1244	bacterial growth	1229:1244	bacterial growth	1229:1244	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	9	42	dep	demonstrate	1512:1522	arg1	ability					1533:1539	the ability	1529:1539	the ability of Acinetobacter spp	1529:1560	Overall, these results demonstrate that the ability of Acinetobacter spp.
35930073	5	43	theme	bees	770:773	arg1	tract					745:749	the digestive tract	731:749	the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	731:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	43	theme	bees	770:773	arg1	nectar					720:725	floral nectar	713:725	floral nectar	713:725	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	3	44	theme	animal	261:266	arg1	visitors					268:275	animal visitors	261:275	animal visitors for their dispersal	261:295	The floral nectar of angiosperms harbors a variety of microorganisms that depend predominantly on animal visitors for their dispersal.
35930073	8	45	theme	nectar	1354:1359	arg1	types					1345:1349	different types	1335:1349	different types of nectar	1335:1359	Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny.
35930073	8	46	theme	bacteria	1315:1322	arg1	ability					1300:1306	the ability	1296:1306	the ability of the bacteria to grow in different types of nectar	1296:1359	Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny.
35930073	5	47	theme	sugar	822:826	arg1	content					828:834	sugar content	822:834	sugar content (15% w/v or 50% w/v)	822:855	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	11	48	theme	nectar	1631:1636	arg1	chemistry					1638:1646	nectar chemistry	1631:1646	nectar chemistry	1631:1646	to grow in floral nectar largely depends on nectar chemistry and bacterial phylogeny.
35930073	5	49	theme	artificial	790:799	arg1	nectars					801:807	12 artificial nectars	787:807	12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	787:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	7	50	contain	containing	1120:1129	arg2	3.48/1.67 ppm					1196:1208	3.48/1.67 ppm	1196:1208	3.48/1.67 ppm	1196:1208	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	50	contain	containing	1120:1129	arg2	w/v					1165:1167	50% w/v	1161:1167	50% w/v	1161:1167	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	50	contain	containing	1120:1129	arg1	Those					1114:1118	Those	1114:1118	Those	1114:1118	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	50	contain	containing	1120:1129	arg2	content					1187:1193	low nitrogen content	1174:1193	low nitrogen content (3.48/1.67 ppm)	1174:1209	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	50	contain	containing	1120:1129	arg2	concentration					1146:1158	elevated sugar concentration	1131:1158	elevated sugar concentration (50% w/v)	1131:1168	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	0	51	theme	Phylogenetic	48:59	arg1	Factors					61:67	Phylogenetic Factors	48:67	Phylogenetic Factors	48:67	Sugar Concentration, Nitrogen Availability, and Phylogenetic Factors Determine the Ability of Acinetobacter spp.
35930073	5	52	theme	floral	713:718	arg1	nectar					720:725	floral nectar	713:725	floral nectar	713:725	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	53	from	nectar	720:725	arg1	set					780:782	a set	778:782	a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	778:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	53	from	nectar	720:725	arg1	nectars					801:807	12 artificial nectars	787:807	12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	787:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	54	theme	only	963:966	arg1	sucrose					968:974	only sucrose	963:974	only sucrose	963:974	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	4	55	theme	Acinetobacter	333:345	arg1	members					312:318	some members	307:318	some members of the genus Acinetobacter	307:345	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	4	55	theme	Acinetobacter	333:345	arg1	species					371:377	all currently known species	351:377	all currently known species of Rosenbergiella	351:395	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	6	56	theme	nectars	1105:1111	arg1	nectars					1105:1111	the 12 artificial nectars	1087:1111	the 12 artificial nectars	1087:1111	Growth was only observed in four of the 12 artificial nectars.
35930073	6	56	theme	nectars	1105:1111	arg1	four					1079:1082	four	1079:1082	four	1079:1082	Growth was only observed in four of the 12 artificial nectars.
35930073	5	57	theme	Rosenbergiella	666:679	arg1	isolates					690:697	Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates	639:697	Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	639:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	6	58	located	observed	1067:1074	arg1	nectars					1105:1111	the 12 artificial nectars	1087:1111	the 12 artificial nectars	1087:1111	Growth was only observed in four of the 12 artificial nectars.
35930073	6	58	located	observed	1067:1074	arg1	four					1079:1082	four	1079:1082	four	1079:1082	Growth was only observed in four of the 12 artificial nectars.
35930073	6	58	located	observed	1067:1074	arg2	Growth					1051:1056	Growth	1051:1056	Growth	1051:1056	Growth was only observed in four of the 12 artificial nectars.
35930073	5	59	theme	1/3	977:979	arg1	sucrose + 1/3					981:993	sucrose + 1/3	981:993	sucrose + 1/3	981:993	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	8	60	theme	different	1335:1343	arg1	types					1345:1349	different types	1335:1349	different types of nectar	1335:1359	Furthermore, phylogenetic analyses revealed that the ability of the bacteria to grow in different types of nectar is highly conserved between closely related isolates and genotypes, but this conservatism rapidly vanishes deeper in phylogeny.
35930073	5	61	theme	growth	593:598	arg1	performance					600:610	the growth performance	589:610	the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	589:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	62	theme	nitrogen	858:865	arg1	content					867:873	nitrogen content	858:873	nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen)	858:937	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	63	theme	sucrose + 1/3	981:993	arg1	fructose					1009:1016	1/3 sucrose + 1/3 glucose + 1/3 fructose	977:1016	1/3 sucrose + 1/3 glucose + 1/3 fructose	977:1016	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	64	dep	content	828:834	arg1	w/v					852:854	15% w/v or 50% w/v	837:854	w/v	852:854	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	64	dep	content	828:834	arg1	w/v					841:843	15% w/v or 50% w/v	837:854	w/v	841:843	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	7	65	theme	nitrogen	1178:1185	arg1	content					1187:1193	low nitrogen content	1174:1193	low nitrogen content (3.48/1.67 ppm)	1174:1209	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	65	theme	nitrogen	1178:1185	arg1	3.48/1.67 ppm					1196:1208	3.48/1.67 ppm	1196:1208	3.48/1.67 ppm	1196:1208	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	5	66	theme	digestive	735:743	arg1	tract					745:749	the digestive tract	731:749	the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	731:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	67	theme	glucose + 1/3	995:1007	arg1	fructose					1009:1016	1/3 sucrose + 1/3 glucose + 1/3 fructose	977:1016	1/3 sucrose + 1/3 glucose + 1/3 fructose	977:1016	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	9	68	theme	spp	1558:1560	arg1	ability					1533:1539	the ability	1529:1539	the ability of Acinetobacter spp	1529:1560	Overall, these results demonstrate that the ability of Acinetobacter spp.
35930073	6	69	theme	artificial	1094:1103	arg1	nectars					1105:1111	the 12 artificial nectars	1087:1111	the 12 artificial nectars	1087:1111	Growth was only observed in four of the 12 artificial nectars.
35930073	4	70	from	variation	513:521	arg1	characteristics					539:553	the chemical characteristics	526:553	the chemical characteristics of floral nectar	526:570	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	5	71	from	bees	770:773	arg1	set					780:782	a set	778:782	a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	778:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	71	from	bees	770:773	arg1	nectars					801:807	12 artificial nectars	787:807	12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	787:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	5	72	theme	sugar	944:948	arg1	composition					950:960	sugar composition	944:960	sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose)	944:1048	We investigated the growth performance of a diverse collection of Acinetobacter (n = 43) and Rosenbergiella (n = 45) isolates obtained from floral nectar and the digestive tract of flower-visiting bees in a set of 12 artificial nectars differing in sugar content (15% w/v or 50% w/v), nitrogen content (3.48/1.67 ppm or 348/167 ppm of total nitrogen/amino nitrogen), and sugar composition (only sucrose, 1/3 sucrose + 1/3 glucose + 1/3 fructose, or 1/2 glucose + 1/2 fructose).
35930073	4	73	theme	genus	327:331	arg1	Acinetobacter					333:345	the genus Acinetobacter	323:345	the genus Acinetobacter	323:345	Although some members of the genus Acinetobacter and all currently known species of Rosenbergiella are thought to be adapted to thrive in nectar, there is limited information about the response of these bacteria to variation in the chemical characteristics of floral nectar.
35930073	7	74	theme	low	1174:1176	arg1	content					1187:1193	low nitrogen content	1174:1193	low nitrogen content (3.48/1.67 ppm)	1174:1209	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
35930073	7	74	theme	low	1174:1176	arg1	3.48/1.67 ppm					1196:1208	3.48/1.67 ppm	1196:1208	3.48/1.67 ppm	1196:1208	Those containing elevated sugar concentration (50% w/v) and low nitrogen content (3.48/1.67 ppm) were limiting for bacterial growth.
36472499	4	0	theme	total	895:899	arg1	collagen					901:908	total collagen	895:908	total collagen	895:908	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	3	1	theme	female	702:707	arg1	pigs					719:722	8 disease-free female Yorkshire pigs	687:722	8 disease-free female Yorkshire pigs (60-70 kg)	687:733	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	3	1	theme	female	702:707	arg1	kg					731:732	60-70 kg	725:732	60-70 kg	725:732	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	2	2	theme	primates	348:355	arg1	use					341:343	the use	337:343	the use of primates	337:355	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	2	theme	primates	348:355	arg1	source					362:367	a source	360:367	a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	360:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	6	3	theme	clinical	1200:1207	arg1	signs					1209:1213	local clinical signs	1194:1213	local clinical signs	1194:1213	Inflammation and adverse events were monitored daily based on local clinical signs.
36472499	10	4	dep	report	1767:1772	arg1	ANS					1792:1794	a novel promising ANS	1774:1794	a preliminary report a novel promising ANS for nipple reconstruction	1753:1820	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	2	5	theme	nipple	275:280	arg1	projection					282:291	nipple projection	275:291	nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	275:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	1	6	theme	autologous	185:194	arg1	flap					201:204	the autologous skin flap	181:204	the autologous skin flap	181:204	BACKGROUND The gold standard in nipple reconstruction remains the autologous skin flap.
36472499	2	7	theme	nipple	621:626	arg1	reconstruction					628:641	nipple reconstruction	621:641	nipple reconstruction	621:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	8	dep	%	265:265	arg1	75					263:264	75	263:264	75	263:264	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	10	9	theme	nipple	1800:1805	arg1	reconstruction					1807:1820	nipple reconstruction	1800:1820	nipple reconstruction	1800:1820	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	2	10	theme	Existing	307:314	arg1	studies					316:322	time.1-8 Existing studies	298:322	time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	298:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	5	11	from	implantation	1057:1068	arg1	pig					1103:1105	a single Yorkshire pig	1084:1105	a single Yorkshire pig (60-70 kg)	1084:1116	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	11	from	implantation	1057:1068	arg1	kg					1114:1115	60-70 kg	1108:1115	60-70 kg	1108:1115	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	12	theme	single	1086:1091	arg1	pig					1103:1105	a single Yorkshire pig	1084:1105	a single Yorkshire pig (60-70 kg)	1084:1116	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	12	theme	single	1086:1091	arg1	kg					1114:1115	60-70 kg	1108:1115	60-70 kg	1108:1115	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	4	13	dep	performed	845:853	arg1	DNA					890:892	DNA	890:892	DNA	890:892	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	2	14	theme	decellularization	545:561	arg1	protocol					563:570	a decellularization protocol	543:570	a decellularization protocol	543:570	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	15	theme	nipple	595:600	arg1	ANS					612:614	ANS	612:614	ANS	612:614	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	15	theme	nipple	595:600	arg1	scaffold					602:609	an acellular nipple scaffold	582:609	an acellular nipple scaffold (ANS) for nipple reconstruction	582:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	8	16	from	increase	1566:1573	arg1	elastin					1508:1514	elastin	1508:1514	elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05))	1508:1560	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	16	from	increase	1566:1573	arg1	collagen					1445:1452	collagen	1445:1452	collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001))	1445:1502	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	16	from	increase	1566:1573	arg1	content					1582:1588	GAG content	1578:1588	GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05))	1578:1627	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	16	from	increase	1566:1573	arg1	collagen/mg					1481:1491	89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)	1455:1501	89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)	1455:1501	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	5	17	theme	ANS	1077:1079	arg1	culture					1032:1038	human dermal fibroblast culture	1008:1038	human dermal fibroblast culture	1008:1038	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	17	theme	ANS	1077:1079	arg1	implantation					1057:1068	subcutaneous implantation	1044:1068	subcutaneous implantation	1044:1068	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	4	18	theme	glycosaminoglycan	924:940	arg1	content					942:948	glycosaminoglycan content	924:948	glycosaminoglycan content	924:948	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	5	19	theme	In	952:953	arg1	biocompatibility					973:988	In vitro and in vivo biocompatibility	952:988	In vitro and in vivo biocompatibility	952:988	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	2	20	theme	human	504:508	arg1	nipple.11,12					510:521	human nipple.11,12	504:521	human nipple.11,12	504:521	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	5	21	dep	in	965:966	arg1	vivo					968:971	vivo	968:971	vivo	968:971	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	7	22	theme	cellular	1243:1250	arg1	structures					1252:1261	all cellular structures	1239:1261	all cellular structures	1239:1261	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	1	23	theme	nipple	151:156	arg1	reconstruction					158:171	nipple reconstruction	151:171	nipple reconstruction	151:171	BACKGROUND The gold standard in nipple reconstruction remains the autologous skin flap.
36472499	9	24	theme	cell	1657:1660	arg1	growth					1662:1667	continuous cell growth	1646:1667	continuous cell growth	1646:1667	ANS can support continuous cell growth in vitro and during preliminary biocompatibility tests in vivo.
36472499	5	25	from	ANS	1077:1079	arg1	pig					1103:1105	a single Yorkshire pig	1084:1105	a single Yorkshire pig (60-70 kg)	1084:1116	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	25	from	ANS	1077:1079	arg1	kg					1114:1115	60-70 kg	1108:1115	60-70 kg	1108:1115	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	1	26	from	reconstruction	158:171	arg1	standard					139:146	standard	139:146	standard	139:146	BACKGROUND The gold standard in nipple reconstruction remains the autologous skin flap.
36472499	1	27	dep	BACKGROUND	119:128	arg1	remains					173:179	remains	173:179	remains the autologous skin flap	173:204	BACKGROUND The gold standard in nipple reconstruction remains the autologous skin flap.
36472499	8	28	from	decrease	1433:1440	arg1	elastin					1508:1514	elastin	1508:1514	elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05))	1508:1560	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	28	from	decrease	1433:1440	arg1	collagen					1445:1452	collagen	1445:1452	collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001))	1445:1502	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	28	from	decrease	1433:1440	arg1	content					1582:1588	GAG content	1578:1588	GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05))	1578:1627	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	28	from	decrease	1433:1440	arg1	collagen/mg					1481:1491	89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)	1455:1501	89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)	1455:1501	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	9	29	theme	preliminary	1689:1699	arg1	tests					1718:1722	preliminary biocompatibility tests	1689:1722	preliminary biocompatibility tests	1689:1722	ANS can support continuous cell growth in vitro and during preliminary biocompatibility tests in vivo.
36472499	2	30	theme	implants.9,10	372:384	arg1	use					341:343	the use	337:343	the use of primates	337:355	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	30	theme	implants.9,10	372:384	arg1	source					362:367	a source	360:367	a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	360:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	0	31	theme	Acellular	0:8	arg1	development					26:36	Acellular nipple scaffold development	0:36	Acellular nipple scaffold development	0:36	Acellular nipple scaffold development, characterization, and preliminary biocompatibility assessment in a swine model.
36472499	0	32	theme	scaffold	17:24	arg1	development					26:36	Acellular nipple scaffold development	0:36	Acellular nipple scaffold development	0:36	Acellular nipple scaffold development, characterization, and preliminary biocompatibility assessment in a swine model.
36472499	7	33	theme	DNA/mg	1307:1312	arg1	tissue					1318:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	33	theme	DNA/mg	1307:1312	arg1	p<0.001					1359:1365	p<0.001	1359:1365	p<0.001	1359:1365	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	0	34	theme	preliminary	61:71	arg1	assessment					90:99	preliminary biocompatibility assessment	61:99	preliminary biocompatibility assessment	61:99	Acellular nipple scaffold development, characterization, and preliminary biocompatibility assessment in a swine model.
36472499	10	35	theme	preliminary	1755:1765	arg1	report					1767:1772	a preliminary report	1753:1772	a preliminary report a novel promising ANS for nipple reconstruction	1753:1820	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	10	35	theme	preliminary	1755:1765	arg1	This					1745:1748	This	1745:1748	This	1745:1748	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	2	36	theme	porcine	407:413	arg1	implant					462:468	a perfect shape-supporting implant	435:468	a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	435:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	36	theme	porcine	407:413	arg1	nipple					415:420	porcine nipple	407:420	porcine nipple	407:420	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	5	37	theme	subcutaneous	1044:1055	arg1	implantation					1057:1068	subcutaneous implantation	1044:1068	subcutaneous implantation	1044:1068	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	3	38	theme	Tissue	652:657	arg1	samples					659:665	METHODS Tissue samples	644:665	METHODS Tissue samples	644:665	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	7	39	dep	RESULTS	1216:1222	arg1	showed					1227:1232	showed	1227:1232	showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed	1227:1394	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	3	40	theme	disease-free	689:700	arg1	pigs					719:722	8 disease-free female Yorkshire pigs	687:722	8 disease-free female Yorkshire pigs (60-70 kg)	687:733	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	3	40	theme	disease-free	689:700	arg1	kg					731:732	60-70 kg	725:732	60-70 kg	725:732	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	2	41	dep	implants.9,10	372:384	arg1	hypothesized					389:400	hypothesized	389:400	hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	389:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	8	42	theme	GAG	1578:1580	arg1	content					1582:1588	GAG content	1578:1588	GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05))	1578:1627	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	5	43	theme	dermal	1014:1019	arg1	culture					1032:1038	human dermal fibroblast culture	1008:1038	human dermal fibroblast culture	1008:1038	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	7	44	theme	DNA	1274:1276	arg1	DNA					1274:1276	DNA	1274:1276	DNA	1274:1276	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	44	theme	DNA	1274:1276	arg1	structures					1252:1261	all cellular structures	1239:1261	all cellular structures	1239:1261	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	44	theme	DNA	1274:1276	arg1	%					1269:1269	96%	1267:1269	96% of DNA	1267:1276	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	0	45	theme	nipple	10:15	arg1	development					26:36	Acellular nipple scaffold development	0:36	Acellular nipple scaffold development	0:36	Acellular nipple scaffold development, characterization, and preliminary biocompatibility assessment in a swine model.
36472499	3	46	theme	Yorkshire	709:717	arg1	pigs					719:722	8 disease-free female Yorkshire pigs	687:722	8 disease-free female Yorkshire pigs (60-70 kg)	687:733	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	3	46	theme	Yorkshire	709:717	arg1	kg					731:732	60-70 kg	725:732	60-70 kg	725:732	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	2	47	theme	projection	282:291	arg1	loss					267:270	up to 75% loss	257:270	up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	257:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	8	48	theme	±	1607:1607	arg1	p<0.05					1620:1625	p<0.05	1620:1625	p<0.05	1620:1625	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	48	theme	±	1607:1607	arg1	ng/mg					1613:1617	5.0±0.7 and 6.0 ± 0.8 ng/mg	1591:1617	5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)	1591:1626	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	6	49	theme	local	1194:1198	arg1	signs					1209:1213	local clinical signs	1194:1213	local clinical signs	1194:1213	Inflammation and adverse events were monitored daily based on local clinical signs.
36472499	5	50	from	culture	1032:1038	arg1	pig					1103:1105	a single Yorkshire pig	1084:1105	a single Yorkshire pig (60-70 kg)	1084:1116	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	50	from	culture	1032:1038	arg1	kg					1114:1115	60-70 kg	1108:1115	60-70 kg	1108:1115	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	1	51	theme	skin	196:199	arg1	flap					201:204	the autologous skin flap	181:204	the autologous skin flap	181:204	BACKGROUND The gold standard in nipple reconstruction remains the autologous skin flap.
36472499	2	52	dep	75	263:264	arg1	to					260:261	to	260:261	to	260:261	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	5	53	theme	Yorkshire	1093:1101	arg1	pig					1103:1105	a single Yorkshire pig	1084:1105	a single Yorkshire pig (60-70 kg)	1084:1116	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	53	theme	Yorkshire	1093:1101	arg1	kg					1114:1115	60-70 kg	1108:1115	60-70 kg	1108:1115	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	10	54	dep	CONCLUSIONS	1733:1743	arg1	report					1767:1772	a preliminary report	1753:1772	a preliminary report a novel promising ANS for nipple reconstruction	1753:1820	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	10	54	dep	CONCLUSIONS	1733:1743	arg1	This					1745:1748	This	1745:1748	This	1745:1748	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	5	55	from	pig	1103:1105	arg1	culture					1032:1038	human dermal fibroblast culture	1008:1038	human dermal fibroblast culture	1008:1038	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	5	55	from	pig	1103:1105	arg1	implantation					1057:1068	subcutaneous implantation	1044:1068	subcutaneous implantation	1044:1068	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	2	56	theme	acellular	585:593	arg1	ANS					612:614	ANS	612:614	ANS	612:614	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	56	theme	acellular	585:593	arg1	scaffold					602:609	an acellular nipple scaffold	582:609	an acellular nipple scaffold (ANS) for nipple reconstruction	582:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	8	57	dep	elastin	1508:1514	arg1	p<0.05					1553:1558	p<0.05	1553:1558	p<0.05	1553:1558	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	57	dep	elastin	1508:1514	arg1	elastin/mg					1541:1550	14.2±1.6 and 7.9±2.4 μg elastin/mg	1517:1550	14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)	1517:1559	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	0	58	theme	biocompatibility	73:88	arg1	assessment					90:99	preliminary biocompatibility assessment	61:99	preliminary biocompatibility assessment	61:99	Acellular nipple scaffold development, characterization, and preliminary biocompatibility assessment in a swine model.
36472499	9	59	theme	continuous	1646:1655	arg1	growth					1662:1667	continuous cell growth	1646:1667	continuous cell growth	1646:1667	ANS can support continuous cell growth in vitro and during preliminary biocompatibility tests in vivo.
36472499	8	60	dep	content	1582:1588	arg1	p<0.05					1620:1625	p<0.05	1620:1625	p<0.05	1620:1625	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	60	dep	content	1582:1588	arg1	ng/mg					1613:1617	5.0±0.7 and 6.0 ± 0.8 ng/mg	1591:1617	5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)	1591:1626	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	2	61	dep	loss	267:270	arg1	%					265:265	%	265:265	%	265:265	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	4	62	dep	efficiency	782:791	arg1	characterization					824:839	characterization	824:839	characterization	824:839	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	2	63	theme	functional	477:486	arg1	similarities					488:499	functional similarities	477:499	functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	477:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	8	64	theme	μg	1478:1479	arg1	collagen					1445:1452	collagen	1445:1452	collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001))	1445:1502	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	64	theme	μg	1478:1479	arg1	collagen/mg					1481:1491	89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)	1455:1501	89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)	1455:1501	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	0	65	dep	development	26:36	arg1	model					112:116	a swine model	104:116	a swine model	104:116	Acellular nipple scaffold development, characterization, and preliminary biocompatibility assessment in a swine model.
36472499	0	66	theme	swine	106:110	arg1	model					112:116	a swine model	104:116	a swine model	104:116	Acellular nipple scaffold development, characterization, and preliminary biocompatibility assessment in a swine model.
36472499	5	67	dep	In	952:953	arg1	vitro					955:959	vitro	955:959	vitro	955:959	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	4	68	theme	decellularization	764:780	arg1	efficiency					782:791	The decellularization efficiency	760:791	The decellularization efficiency	760:791	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	4	69	dep	DNA	890:892	arg1	elastin					911:917	elastin	911:917	elastin	911:917	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	4	69	dep	DNA	890:892	arg1	collagen					901:908	total collagen	895:908	total collagen	895:908	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	4	69	dep	DNA	890:892	arg1	content					942:948	glycosaminoglycan content	924:948	glycosaminoglycan content	924:948	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	1	70	from	standard	139:146	arg1	reconstruction					158:171	nipple reconstruction	151:171	nipple reconstruction	151:171	BACKGROUND The gold standard in nipple reconstruction remains the autologous skin flap.
36472499	7	71	dep	structures	1252:1261	arg1	tissue					1318:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	71	dep	structures	1252:1261	arg1	p<0.001					1359:1365	p<0.001	1359:1365	p<0.001	1359:1365	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	72	theme	wet	1314:1316	arg1	tissue					1318:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	72	theme	wet	1314:1316	arg1	p<0.001					1359:1365	p<0.001	1359:1365	p<0.001	1359:1365	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	9	73	theme	biocompatibility	1701:1716	arg1	tests					1718:1722	preliminary biocompatibility tests	1689:1722	preliminary biocompatibility tests	1689:1722	ANS can support continuous cell growth in vitro and during preliminary biocompatibility tests in vivo.
36472499	5	74	theme	in	965:966	arg1	biocompatibility					973:988	In vitro and in vivo biocompatibility	952:988	In vitro and in vivo biocompatibility	952:988	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	7	75	theme	ng	1304:1305	arg1	tissue					1318:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue	1279:1323	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	75	theme	ng	1304:1305	arg1	p<0.001					1359:1365	p<0.001	1359:1365	p<0.001	1359:1365	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	4	76	theme	extracellular	797:809	arg1	ECM					819:821	ECM	819:821	ECM	819:821	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	4	76	theme	extracellular	797:809	arg1	matrix					811:816	extracellular matrix	797:816	extracellular matrix (ECM)	797:822	The decellularization efficiency and extracellular matrix (ECM) characterization was performed histologically and quantitatively (DNA, total collagen, elastin, and glycosaminoglycan content).
36472499	2	77	theme	due	470:472	arg1	implant					462:468	a perfect shape-supporting implant	435:468	a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	435:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	77	theme	due	470:472	arg1	nipple					415:420	porcine nipple	407:420	porcine nipple	407:420	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	3	78	theme	METHODS	644:650	arg1	samples					659:665	METHODS Tissue samples	644:665	METHODS Tissue samples	644:665	METHODS Tissue samples were collected from 8 disease-free female Yorkshire pigs (60-70 kg) and then decellularized.
36472499	2	79	dep	similarities	488:499	arg1	developed					533:541	developed	533:541	developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	533:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	6	80	theme	adverse	1149:1155	arg1	events					1157:1162	adverse events	1149:1162	adverse events	1149:1162	Inflammation and adverse events were monitored daily based on local clinical signs.
36472499	2	81	theme	shape-supporting	445:460	arg1	implant					462:468	a perfect shape-supporting implant	435:468	a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	435:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	81	theme	shape-supporting	445:460	arg1	nipple					415:420	porcine nipple	407:420	porcine nipple	407:420	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	8	82	theme	μg	1538:1539	arg1	p<0.05					1553:1558	p<0.05	1553:1558	p<0.05	1553:1558	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	8	82	theme	μg	1538:1539	arg1	elastin/mg					1541:1550	14.2±1.6 and 7.9±2.4 μg elastin/mg	1517:1550	14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)	1517:1559	However, this was associated with a decrease in collagen (89.0±11.4 and 58.8±9.6 μg collagen/mg (p<0.001)) and elastin (14.2±1.6 and 7.9±2.4 μg elastin/mg (p<0.05)) and increase in GAG content (5.0±0.7 and 6.0 ± 0.8 ng/mg (p<0.05)).
36472499	5	83	theme	human	1008:1012	arg1	culture					1032:1038	human dermal fibroblast culture	1008:1038	human dermal fibroblast culture	1008:1038	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	10	84	dep	novel	1776:1780	arg1	promising					1782:1790	promising	1782:1790	promising	1782:1790	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	10	85	theme	novel	1776:1780	arg1	ANS					1792:1794	a novel promising ANS	1774:1794	a preliminary report a novel promising ANS for nipple reconstruction	1753:1820	CONCLUSIONS This is a preliminary report a novel promising ANS for nipple reconstruction, but more research is needed to validate results.
36472499	5	86	theme	fibroblast	1021:1030	arg1	culture					1032:1038	human dermal fibroblast culture	1008:1038	human dermal fibroblast culture	1008:1038	In vitro and in vivo biocompatibility was determined by human dermal fibroblast culture and subcutaneous implantation of six ANS in a single Yorkshire pig (60-70 kg) respectively.
36472499	7	87	from	tissue	1318:1323	arg1	ANS					1340:1342	ANS	1340:1342	ANS	1340:1342	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	7	87	from	tissue	1318:1323	arg1	native					1329:1334	native	1329:1334	native	1329:1334	RESULTS We showed that all cellular structures and 96% of DNA (321.7±57.6 vs. 11.7±10.9 ng DNA/mg wet tissue, in native and ANS, respectively, p<0.001) can be successfully removed.
36472499	2	88	theme	perfect	437:443	arg1	implant					462:468	a perfect shape-supporting implant	435:468	a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction	435:641	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
36472499	2	88	theme	perfect	437:443	arg1	nipple					415:420	porcine nipple	407:420	porcine nipple	407:420	Unfortunately the results are not satisfying with up to 75% loss of nipple projection over time.1-8 Existing studies investigated the use of primates as a source of implants.9,10 We hypothesized that porcine nipple can serve as a perfect shape-supporting implant due to functional similarities to human nipple.11,12 Our group developed a decellularization protocol to obtain an acellular nipple scaffold (ANS) for nipple reconstruction.
35762582	1	0	theme	cell	241:244	arg1	peptidoglycan					251:263	cell wall peptidoglycan	241:263	cell wall peptidoglycan	241:263	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	9	1	theme	multiple	1780:1787	arg1	pressures					1801:1809	multiple independent pressures	1780:1809	multiple independent pressures	1780:1809	In total, our work reveals that Tae diversification has likely been shaped by multiple independent pressures to maintain interactions with binding partners that vary across bacterial species and conditions.
35762582	3	2	from	PAO1	541:544	arg1	determinants					492:503	the functional determinants	477:503	the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1	477:544	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	3	3	theme	nuclear	569:575	arg1	resonance					586:594	nuclear magnetic resonance	569:594	nuclear magnetic resonance	569:594	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	0	4	theme	Antibacterial	0:12	arg1	potency					14:20	Antibacterial potency	0:20	Antibacterial potency of type VI amidase effector toxins	0:55	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	1	5	theme	Tae	162:164	arg1	superfamily					167:177	the bacterial T6SS amidase effector (Tae) superfamily	125:177	the bacterial T6SS amidase effector (Tae) superfamily of toxins	125:187	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	1	6	theme	superfamily	167:177	arg1	Members					114:120	Members	114:120	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins	114:187	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	3	7	theme	resonance	586:594	arg1	combination					554:564	a combination	552:564	a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS)	552:679	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	1	8	theme	toxins	182:187	arg1	superfamily					167:177	the bacterial T6SS amidase effector (Tae) superfamily	125:177	the bacterial T6SS amidase effector (Tae) superfamily of toxins	125:187	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	6	9	theme	distinct	990:997	arg1	conditions					1006:1015	distinct growth conditions	990:1015	distinct growth conditions	990:1015	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	3	10	theme	genetic	626:632	arg1	approach					634:641	a high-throughput in vivo genetic approach	600:641	a high-throughput in vivo genetic approach called deep mutational scanning (DMS)	600:679	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	3	11	dep	in	618:619	arg1	vivo					621:624	vivo	621:624	vivo	621:624	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	8	12	theme	Tae	1599:1601	arg1	activity					1603:1610	Tae activity	1599:1610	Tae activity	1599:1610	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	9	13	theme	bacterial	1875:1883	arg1	species					1885:1891	bacterial species	1875:1891	bacterial species	1875:1891	In total, our work reveals that Tae diversification has likely been shaped by multiple independent pressures to maintain interactions with binding partners that vary across bacterial species and conditions.
35762582	4	14	theme	residues	744:751	arg1	role					727:730	the role	723:730	the role of critical residues near the Tae1 catalytic center	723:782	As expected, combined analyses confirmed the role of critical residues near the Tae1 catalytic center.
35762582	3	15	theme	mutational	655:664	arg1	DMS					676:678	DMS	676:678	DMS	676:678	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	3	15	theme	mutational	655:664	arg1	scanning					666:673	deep mutational scanning	650:673	deep mutational scanning (DMS)	650:679	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	5	16	theme	enzyme	959:964	arg1	circumference					938:950	the entire circumference	927:950	the entire circumference of the enzyme	927:964	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	1	17	theme	T6SS	139:142	arg1	superfamily					167:177	the bacterial T6SS amidase effector (Tae) superfamily	125:177	the bacterial T6SS amidase effector (Tae) superfamily of toxins	125:187	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	6	18	theme	different	1060:1068	arg1	surfaces					1070:1077	different surfaces	1060:1077	different surfaces	1060:1077	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	8	19	theme	Further	1456:1462	arg1	studies					1472:1478	Further binding studies	1456:1478	Further binding studies of several Tae1 variants with their cognate immunity protein	1456:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	1	20	theme	effector	152:159	arg1	superfamily					167:177	the bacterial T6SS amidase effector (Tae) superfamily	125:177	the bacterial T6SS amidase effector (Tae) superfamily of toxins	125:187	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	8	21	with	variants	1496:1503	arg1	protein					1533:1539	their cognate immunity protein	1510:1539	their cognate immunity protein	1510:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	7	22	theme	interaction	1286:1296	arg1	interface					1298:1306	a more distributed three-dimensional interaction interface	1249:1306	a more distributed three-dimensional interaction interface than previously appreciated	1249:1334	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	8	23	theme	several	1483:1489	arg1	variants					1496:1503	several Tae1 variants	1483:1503	several Tae1 variants with their cognate immunity protein	1483:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	0	24	theme	cellular	96:103	arg1	context					105:111	cellular context	96:111	cellular context	96:111	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	0	25	theme	amidase	33:39	arg1	toxins					50:55	type VI amidase effector toxins	25:55	type VI amidase effector toxins	25:55	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	6	26	theme	targeted	1141:1148	arg1	walls					1155:1159	targeted cell walls	1141:1159	targeted cell walls	1141:1159	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	8	27	theme	tae1	1675:1678	arg1	toxicity					1680:1687	tae1 toxicity	1675:1687	tae1 toxicity	1675:1687	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	8	28	theme	variants	1496:1503	arg1	studies					1472:1478	Further binding studies	1456:1478	Further binding studies of several Tae1 variants with their cognate immunity protein	1456:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	5	29	theme	enzymatic	841:849	arg1	activity					851:858	enzymatic activity	841:858	enzymatic activity	841:858	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	7	30	dep	interface	1298:1306	arg1	appreciated					1324:1334	appreciated	1324:1334	appreciated	1324:1334	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	4	31	theme	catalytic	767:775	arg1	center					777:782	the Tae1 catalytic center	758:782	the Tae1 catalytic center	758:782	As expected, combined analyses confirmed the role of critical residues near the Tae1 catalytic center.
35762582	8	32	from	constraint	1633:1642	arg1	wild					1696:1699	wild	1696:1699	wild	1696:1699	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	8	32	from	constraint	1633:1642	arg1	landscape					1662:1670	the mutational landscape	1647:1670	the mutational landscape of tae1 toxicity	1647:1687	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	7	33	theme	divergent	1419:1427	arg1	competitors					1443:1453	divergent Gram-negative competitors	1419:1453	divergent Gram-negative competitors	1419:1453	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	8	34	theme	immunity	1524:1531	arg1	protein					1533:1539	their cognate immunity protein	1510:1539	their cognate immunity protein	1510:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	5	35	theme	ring-like	880:888	arg1	spot					905:908	a much larger, ring-like functional hot spot	865:908	spot	905:908	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	7	36	theme	cell	1223:1226	arg1	network					1233:1239	the intact cell wall network	1212:1239	the intact cell wall network	1212:1239	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	3	37	theme	Tae1	508:511	arg1	determinants					492:503	the functional determinants	477:503	the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1	477:544	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	0	38	theme	effector	41:48	arg1	toxins					50:55	type VI amidase effector toxins	25:55	type VI amidase effector toxins	25:55	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	9	39	theme	Tae	1734:1736	arg1	diversification					1738:1752	Tae diversification	1734:1752	Tae diversification	1734:1752	In total, our work reveals that Tae diversification has likely been shaped by multiple independent pressures to maintain interactions with binding partners that vary across bacterial species and conditions.
35762582	7	40	theme	observed	1366:1373	arg1	differences					1375:1385	observed differences	1366:1385	observed differences in antimicrobial potency across divergent Gram-negative competitors	1366:1453	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	2	41	theme	competitor	367:376	arg1	species					378:384	different competitor species	357:384	different competitor species	357:384	Although Taes share a common substrate, they exhibit distinct antimicrobial potency across different competitor species.
35762582	5	42	theme	functional	890:899	arg1	spot					905:908	a much larger, ring-like functional hot spot	865:908	spot	905:908	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	5	43	theme	substantial	812:822	arg1	contributions					824:836	substantial contributions	812:836	substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme	812:964	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	3	44	theme	Pseudomonas	518:528	arg1	PAO1					541:544	Pseudomonas aeruginosa PAO1	518:544	Pseudomonas aeruginosa PAO1	518:544	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	6	45	theme	walls	1155:1159	arg1	composition					1126:1136	alongside composition	1116:1136	alongside composition of targeted cell walls	1116:1159	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	1	46	theme	wall	246:249	arg1	peptidoglycan					251:263	cell wall peptidoglycan	241:263	cell wall peptidoglycan	241:263	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	9	47	theme	independent	1789:1799	arg1	pressures					1801:1809	multiple independent pressures	1780:1809	multiple independent pressures	1780:1809	In total, our work reveals that Tae diversification has likely been shaped by multiple independent pressures to maintain interactions with binding partners that vary across bacterial species and conditions.
35762582	2	48	theme	antimicrobial	328:340	arg1	potency					342:348	distinct antimicrobial potency	319:348	distinct antimicrobial potency	319:348	Although Taes share a common substrate, they exhibit distinct antimicrobial potency across different competitor species.
35762582	3	49	dep	high-throughput	602:616	arg1	in					618:619	in	618:619	in	618:619	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	5	50	theme	hot	901:903	arg1	spot					905:908	a much larger, ring-like functional hot spot	865:908	spot	905:908	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	7	51	from	differences	1375:1385	arg1	potency					1404:1410	antimicrobial potency	1390:1410	antimicrobial potency across divergent Gram-negative competitors	1390:1453	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	2	52	theme	common	288:293	arg1	substrate					295:303	a common substrate	286:303	a common substrate	286:303	Although Taes share a common substrate, they exhibit distinct antimicrobial potency across different competitor species.
35762582	6	53	theme	growth	999:1004	arg1	conditions					1006:1015	distinct growth conditions	990:1015	distinct growth conditions	990:1015	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	9	54	theme	binding	1841:1847	arg1	partners					1849:1856	binding partners	1841:1856	binding partners that vary across bacterial species and conditions	1841:1906	In total, our work reveals that Tae diversification has likely been shaped by multiple independent pressures to maintain interactions with binding partners that vary across bacterial species and conditions.
35762582	5	55	theme	entire	931:936	arg1	circumference					938:950	the entire circumference	927:950	the entire circumference of the enzyme	927:964	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	9	56	with	interactions	1823:1834	arg1	partners					1849:1856	binding partners	1841:1856	binding partners that vary across bacterial species and conditions	1841:1906	In total, our work reveals that Tae diversification has likely been shaped by multiple independent pressures to maintain interactions with binding partners that vary across bacterial species and conditions.
35762582	5	57	theme	larger	872:877	arg1	spot					905:908	a much larger, ring-like functional hot spot	865:908	spot	905:908	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	3	58	theme	high-throughput	602:616	arg1	approach					634:641	a high-throughput in vivo genetic approach	600:641	a high-throughput in vivo genetic approach called deep mutational scanning (DMS)	600:679	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	6	59	theme	Comparative	967:977	arg1	DMS					979:981	Comparative DMS	967:981	Comparative DMS across distinct growth conditions	967:1015	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	7	60	theme	wall	1228:1231	arg1	network					1233:1239	the intact cell wall network	1212:1239	the intact cell wall network	1212:1239	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	3	61	theme	deep	650:653	arg1	DMS					676:678	DMS	676:678	DMS	676:678	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	3	61	theme	deep	650:653	arg1	scanning					666:673	deep mutational scanning	650:673	deep mutational scanning (DMS)	650:679	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	8	62	dep	requirements	1558:1569	arg1	maintain					1574:1581	maintain	1574:1581	to maintain protection from Tae activity	1571:1610	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	3	63	theme	magnetic	577:584	arg1	resonance					586:594	nuclear magnetic resonance	569:594	nuclear magnetic resonance	569:594	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	8	64	theme	significant	1621:1631	arg1	requirements					1558:1569	requirements	1558:1569	requirements to maintain protection from Tae activity	1558:1610	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	8	64	theme	significant	1621:1631	arg1	constraint					1633:1642	a significant constraint	1619:1642	a significant constraint on the mutational landscape of tae1 toxicity	1619:1687	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	4	65	theme	critical	735:742	arg1	residues					744:751	critical residues	735:751	critical residues near the Tae1 catalytic center	735:782	As expected, combined analyses confirmed the role of critical residues near the Tae1 catalytic center.
35762582	8	66	from	activity	1603:1610	arg1	protection					1583:1592	protection	1583:1592	protection from Tae activity	1583:1610	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	3	67	theme	approach	634:641	arg1	combination					554:564	a combination	552:564	a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS)	552:679	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	5	68	from	spot	905:908	arg1	contributions					824:836	substantial contributions	812:836	substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme	812:964	Unexpectedly, DMS revealed substantial contributions to enzymatic activity from a much larger, ring-like functional hot spot extending around the entire circumference of the enzyme.
35762582	1	69	theme	bacterial	129:137	arg1	superfamily					167:177	the bacterial T6SS amidase effector (Tae) superfamily	125:177	the bacterial T6SS amidase effector (Tae) superfamily of toxins	125:187	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	6	70	theme	surfaces	1070:1077	arg1	contribution					1044:1055	functional contribution	1033:1055	functional contribution of different surfaces	1033:1077	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	4	71	theme	combined	695:702	arg1	analyses					704:711	combined analyses	695:711	combined analyses	695:711	As expected, combined analyses confirmed the role of critical residues near the Tae1 catalytic center.
35762582	0	72	theme	substrate	73:81	arg1	topology					83:90	substrate topology	73:90	substrate topology	73:90	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	1	73	theme	amidase	144:150	arg1	superfamily					167:177	the bacterial T6SS amidase effector (Tae) superfamily	125:177	the bacterial T6SS amidase effector (Tae) superfamily of toxins	125:187	Members of the bacterial T6SS amidase effector (Tae) superfamily of toxins are delivered between competing bacteria to degrade cell wall peptidoglycan.
35762582	8	74	theme	mutational	1651:1660	arg1	landscape					1662:1670	the mutational landscape	1647:1670	the mutational landscape of tae1 toxicity	1647:1687	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	7	75	theme	three-dimensional	1268:1284	arg1	interface					1298:1306	a more distributed three-dimensional interaction interface	1249:1306	a more distributed three-dimensional interaction interface than previously appreciated	1249:1334	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	6	76	theme	functional	1033:1042	arg1	contribution					1044:1055	functional contribution	1033:1055	functional contribution of different surfaces	1033:1077	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	8	77	theme	binding	1464:1470	arg1	studies					1472:1478	Further binding studies	1456:1478	Further binding studies of several Tae1 variants with their cognate immunity protein	1456:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	6	78	theme	cell	1150:1153	arg1	walls					1155:1159	targeted cell walls	1141:1159	targeted cell walls	1141:1159	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	8	79	theme	toxicity	1680:1687	arg1	landscape					1662:1670	the mutational landscape	1647:1670	the mutational landscape of tae1 toxicity	1647:1687	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	3	80	dep	Pseudomonas	518:528	arg1	aeruginosa					530:539	aeruginosa	530:539	aeruginosa	530:539	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	7	81	theme	Gram-negative	1429:1441	arg1	competitors					1443:1453	divergent Gram-negative competitors	1419:1453	divergent Gram-negative competitors	1419:1453	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	8	82	theme	Tae1	1491:1494	arg1	variants					1496:1503	several Tae1 variants	1483:1503	several Tae1 variants with their cognate immunity protein	1483:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	0	83	theme	type	25:28	arg1	toxins					50:55	type VI amidase effector toxins	25:55	type VI amidase effector toxins	25:55	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	6	84	theme	alongside	1116:1124	arg1	composition					1126:1136	alongside composition	1116:1136	alongside composition of targeted cell walls	1116:1159	Comparative DMS across distinct growth conditions highlighted how functional contribution of different surfaces is highly context-dependent, varying alongside composition of targeted cell walls.
35762582	4	85	theme	Tae1	762:765	arg1	center					777:782	the Tae1 catalytic center	758:782	the Tae1 catalytic center	758:782	As expected, combined analyses confirmed the role of critical residues near the Tae1 catalytic center.
35762582	3	86	theme	functional	481:490	arg1	determinants					492:503	the functional determinants	477:503	the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1	477:544	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	7	87	theme	intact	1216:1221	arg1	network					1233:1239	the intact cell wall network	1212:1239	the intact cell wall network	1212:1239	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	0	88	dep	toxins	50:55	arg1	VI					30:31	VI	30:31	VI	30:31	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	7	89	theme	antimicrobial	1390:1402	arg1	potency					1404:1410	antimicrobial potency	1390:1410	antimicrobial potency across divergent Gram-negative competitors	1390:1453	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	8	90	theme	cognate	1516:1522	arg1	protein					1533:1539	their cognate immunity protein	1510:1539	their cognate immunity protein	1510:1539	Further binding studies of several Tae1 variants with their cognate immunity protein demonstrate that requirements to maintain protection from Tae activity may be a significant constraint on the mutational landscape of tae1 toxicity in the wild.
35762582	7	91	theme	distributed	1256:1266	arg1	interface					1298:1306	a more distributed three-dimensional interaction interface	1249:1306	a more distributed three-dimensional interaction interface than previously appreciated	1249:1334	These observations suggest that Tae1 engages with the intact cell wall network through a more distributed three-dimensional interaction interface than previously appreciated, providing an explanation for observed differences in antimicrobial potency across divergent Gram-negative competitors.
35762582	0	92	theme	toxins	50:55	arg1	potency					14:20	Antibacterial potency	0:20	Antibacterial potency of type VI amidase effector toxins	0:55	Antibacterial potency of type VI amidase effector toxins is dependent on substrate topology and cellular context.
35762582	3	93	theme	molecular	406:414	arg1	basis					416:420	the molecular basis	402:420	the molecular basis governing these differences	402:448	To investigate the molecular basis governing these differences, we quantitatively defined the functional determinants of Tae1 from Pseudomonas aeruginosa PAO1 using a combination of nuclear magnetic resonance and a high-throughput in vivo genetic approach called deep mutational scanning (DMS).
35762582	2	94	theme	distinct	319:326	arg1	potency					342:348	distinct antimicrobial potency	319:348	distinct antimicrobial potency	319:348	Although Taes share a common substrate, they exhibit distinct antimicrobial potency across different competitor species.
35762582	2	95	theme	different	357:365	arg1	species					378:384	different competitor species	357:384	different competitor species	357:384	Although Taes share a common substrate, they exhibit distinct antimicrobial potency across different competitor species.
35090002	9	0	from	year	1741:1744	arg1	fat					1721:1723	percentage total body fat	1699:1723	percentage total body fat at baseline and year 2	1699:1746	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	2	1	theme	DXA	597:599	arg1	months					561:566	3 months	559:566	3 months of baseline DXA and a second DXA 2 years later	559:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	2	theme	dysfunction	674:684	arg1	markers					623:629	Plasma markers	616:629	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP])	616:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	4	3	from	body	921:924	arg1	DXA					972:974	DXA	972:974	DXA	972:974	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	4	3	from	body	921:924	arg1	kilograms					957:965	kilograms	957:965	kilograms from DXA	957:974	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	4	4	from	fat	950:952	arg1	DXA					972:974	DXA	972:974	DXA	972:974	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	4	4	from	fat	950:952	arg1	kilograms					957:965	kilograms	957:965	kilograms from DXA	957:974	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	9	5	from	baseline	1728:1735	arg1	fat					1721:1723	percentage total body fat	1699:1723	percentage total body fat at baseline and year 2	1699:1746	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	4	6	theme	Adiposity	876:884	arg1	outcomes					886:893	Adiposity outcomes	876:893	Adiposity outcomes	876:893	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	8	7	theme	time	1622:1625	arg1	points					1627:1632	both time points	1617:1632	both time points	1617:1632	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	5	8	theme	therapy	1211:1217	arg1	exposure					1219:1226	current antiretroviral therapy exposure	1188:1226	current antiretroviral therapy exposure	1188:1226	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	2	9	with	Protocol	415:422	arg1	plasma					429:434	plasma	429:434	plasma	429:434	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	10	10	theme	viral	1814:1818	arg1	suppression					1820:1830	viral suppression	1814:1830	viral suppression	1814:1830	CONCLUSIONS Despite viral suppression, gut damage and the resultant bacterial translocation are associated with body composition measures in YPHIV.
35090002	8	11	from	zonulin	1582:1588	arg1	fat					1542:1544	percentage total body fat	1520:1544	percentage total body fat at baseline (β = 4.08, P < .01) and zonulin	1520:1588	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	1	12	theme	body	188:191	arg1	composition					197:207	body fat composition	188:207	body fat composition	188:207	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	7	13	dep	years	1356:1360	arg1	range					1377:1381	interquartile range	1363:1381	interquartile range	1363:1381	Median age at first DXA was 12 years (interquartile range, 10-14 years), 49% were female, and 69% were Black.
35090002	7	13	dep	years	1356:1360	arg1	years					1390:1394	10-14 years	1384:1394	10-14 years	1384:1394	Median age at first DXA was 12 years (interquartile range, 10-14 years), 49% were female, and 69% were Black.
35090002	3	14	theme	zonulin	729:735	arg1	markers					623:629	Plasma markers	616:629	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP])	616:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	2	15	theme	copies/mL	542:550	arg1	months					455:460	6 months	453:460	6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	453:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	16	theme	binding	764:770	arg1	protein					772:778	intestinal fatty acid binding protein	742:778	intestinal fatty acid binding protein [I-FABP])	742:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	5	17	theme	current	1188:1194	arg1	therapy					1211:1217	current antiretroviral therapy	1188:1217	current antiretroviral therapy exposure	1188:1226	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	5	18	theme	gut	1085:1087	arg1	log10					1098:1102	log10	1098:1102	log10	1098:1102	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	5	18	theme	gut	1085:1087	arg1	markers					1089:1095	baseline gut markers	1076:1095	baseline gut markers (log10)	1076:1103	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	10	19	theme	resultant	1852:1860	arg1	translocation					1872:1884	the resultant bacterial translocation	1848:1884	the resultant bacterial translocation	1848:1884	CONCLUSIONS Despite viral suppression, gut damage and the resultant bacterial translocation are associated with body composition measures in YPHIV.
35090002	2	20	theme	DXA	580:582	arg1	months					561:566	3 months	559:566	3 months of baseline DXA and a second DXA 2 years later	559:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	21	dep	METHODS	314:320	arg1	included					325:332	included	325:332	included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	325:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	22	theme	fatty	753:757	arg1	protein					772:778	intestinal fatty acid binding protein	742:778	intestinal fatty acid binding protein [I-FABP])	742:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	4	23	theme	total	915:919	arg1	body					921:924	percentage total body, truncal, and extremity fat	904:952	body	921:924	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	10	24	theme	composition	1911:1921	arg1	measures					1923:1930	body composition measures	1906:1930	body composition measures in YPHIV	1906:1939	CONCLUSIONS Despite viral suppression, gut damage and the resultant bacterial translocation are associated with body composition measures in YPHIV.
35090002	8	25	from	baseline	1549:1556	arg1	fat					1542:1544	percentage total body fat	1520:1544	percentage total body fat at baseline (β = 4.08, P < .01) and zonulin	1520:1588	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	3	26	theme	enzyme-linked	819:831	arg1	assay					847:851	enzyme-linked immunosorbent assay	819:851	enzyme-linked immunosorbent assay	819:851	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	7	27	theme	Median	1325:1330	arg1	age					1332:1334	Median age	1325:1334	Median age at first DXA	1325:1347	Median age at first DXA was 12 years (interquartile range, 10-14 years), 49% were female, and 69% were Black.
35090002	5	28	from	associations	1060:1071	arg1	years					1145:1149	2 years	1143:1149	2 years	1143:1149	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	5	28	from	associations	1060:1071	arg1	outcomes					1118:1125	adiposity outcomes	1108:1125	adiposity outcomes at baseline	1108:1137	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	5	29	theme	adiposity	1108:1116	arg1	outcomes					1118:1125	adiposity outcomes	1108:1125	adiposity outcomes at baseline	1108:1137	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	2	30	theme	absorptiometry	503:516	arg1	months					455:460	6 months	453:460	6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	453:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	9	31	dep	β = -2.36	1749:1757	arg1	P ≤ .02					1784:1790	P ≤ .02	1784:1790	P ≤ .02	1784:1790	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	2	32	theme	RNA	532:534	arg1	copies/mL					542:550	HIV RNA ≤1000 copies/mL	528:550	HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	528:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	8	33	dep	6.50	1646:1649	arg1	to					1643:1644	to	1643:1644	to	1643:1644	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	8	33	dep	6.50	1646:1649	arg1	P ≤ .01					1652:1658	P ≤ .01	1652:1658	P ≤ .01	1652:1658	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	1	34	dep	BACKGROUND	133:142	arg1	investigated					300:311	investigated	300:311	has not been investigated	287:311	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	1	35	from	association	148:158	arg1	youth					212:216	youth	212:216	youth living with perinatal human immunodeficiency virus infection (YPHIV)	212:285	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	2	36	theme	Master	408:413	arg1	Protocol					415:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol	361:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma	361:434	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	0	37	theme	Human	103:107	arg1	Virus					126:130	Perinatally Acquired Human Immunodeficiency Virus	82:130	Perinatally Acquired Human Immunodeficiency Virus	82:130	Gut Dysfunction Markers Are Associated With Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus.
35090002	9	38	theme	percentage	1699:1708	arg1	fat					1721:1723	percentage total body fat	1699:1723	percentage total body fat at baseline and year 2	1699:1746	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	8	39	theme	potential	1456:1464	arg1	confounders					1466:1476	potential confounders	1456:1476	potential confounders	1456:1476	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	2	40	dep	aged	340:343	arg1	years					350:354	7-19 years	345:354	7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	345:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	41	theme	Study	391:395	arg1	Protocol					415:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol	361:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma	361:434	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	0	42	theme	Gut	0:2	arg1	Dysfunction					4:14	Gut Dysfunction	0:14	Gut Dysfunction Markers	0:22	Gut Dysfunction Markers Are Associated With Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus.
35090002	7	43	from	DXA	1345:1347	arg1	age					1332:1334	Median age	1325:1334	Median age at first DXA	1325:1347	Median age at first DXA was 12 years (interquartile range, 10-14 years), 49% were female, and 69% were Black.
35090002	9	44	theme	body	1716:1719	arg1	fat					1721:1723	percentage total body fat	1699:1723	percentage total body fat at baseline and year 2	1699:1746	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	2	45	theme	available	436:444	arg1	years					350:354	7-19 years	345:354	7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	345:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	5	46	theme	demographic	1165:1175	arg1	variables					1177:1185	demographic variables	1165:1185	demographic variables	1165:1185	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	2	47	theme	energy	490:495	arg1	DXA					519:521	DXA	519:521	DXA	519:521	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	47	theme	energy	490:495	arg1	absorptiometry					503:516	baseline whole-body dual energy x-ray absorptiometry	465:516	baseline whole-body dual energy x-ray absorptiometry (DXA)	465:522	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	48	theme	bacterial	634:642	arg1	translocation					644:656	bacterial translocation	634:656	bacterial translocation	634:656	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	5	49	from	baseline	1130:1137	arg1	years					1145:1149	2 years	1143:1149	2 years	1143:1149	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	5	49	from	baseline	1130:1137	arg1	outcomes					1118:1125	adiposity outcomes	1108:1125	adiposity outcomes at baseline	1108:1137	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	2	50	theme	whole-body	474:483	arg1	DXA					519:521	DXA	519:521	DXA	519:521	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	50	theme	whole-body	474:483	arg1	absorptiometry					503:516	baseline whole-body dual energy x-ray absorptiometry	465:516	baseline whole-body dual energy x-ray absorptiometry (DXA)	465:522	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	0	51	theme	Body	44:47	arg1	Composition					49:59	Body Composition	44:59	Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus	44:130	Gut Dysfunction Markers Are Associated With Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus.
35090002	10	52	from	measures	1923:1930	arg1	YPHIV					1935:1939	YPHIV	1935:1939	YPHIV	1935:1939	CONCLUSIONS Despite viral suppression, gut damage and the resultant bacterial translocation are associated with body composition measures in YPHIV.
35090002	5	53	theme	estimating	1029:1038	arg1	equations					1040:1048	generalized estimating equations	1017:1048	generalized estimating equations	1017:1048	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	3	54	theme	barrier	666:672	arg1	dysfunction					674:684	gut barrier dysfunction	662:684	gut barrier dysfunction	662:684	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	2	55	theme	aged	340:343	arg1	YPHIV					334:338	YPHIV	334:338	YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	334:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	1	56	theme	perinatal	230:238	arg1	YPHIV					280:284	YPHIV	280:284	YPHIV	280:284	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	1	56	theme	perinatal	230:238	arg1	infection					269:277	perinatal human immunodeficiency virus infection	230:277	perinatal human immunodeficiency virus infection (YPHIV)	230:285	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	5	57	theme	regression	984:993	arg1	models					995:1000	Linear regression models	977:1000	Linear regression models	977:1000	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	8	58	theme	total	1531:1535	arg1	fat					1542:1544	percentage total body fat	1520:1544	percentage total body fat at baseline (β = 4.08, P < .01) and zonulin	1520:1588	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	2	59	theme	HIV/AIDS	375:382	arg1	Protocol					415:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol	361:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma	361:434	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	1	60	theme	immunodeficiency	246:261	arg1	virus					263:267	human immunodeficiency virus	240:267	perinatal human immunodeficiency virus infection (YPHIV)	230:285	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	3	61	dep	translocation	644:656	arg1	binding					706:712	lipopolysaccharide binding	687:712	lipopolysaccharide binding protein [LBP	687:725	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	6	62	dep	RESULTS	1252:1258	arg1	youth					1282:1286	hundred sixty-one youth	1264:1286	RESULTS Two hundred sixty-one youth	1252:1286	RESULTS Two hundred sixty-one youth were included; 128 had a second DXA.
35090002	2	63	theme	second	590:595	arg1	DXA					597:599	a second DXA	588:599	a second DXA 2 years later	588:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	6	64	theme	sixty-one	1272:1280	arg1	youth					1282:1286	hundred sixty-one youth	1264:1286	RESULTS Two hundred sixty-one youth	1252:1286	RESULTS Two hundred sixty-one youth were included; 128 had a second DXA.
35090002	5	65	theme	antiretroviral	1196:1209	arg1	therapy					1211:1217	current antiretroviral therapy	1188:1217	current antiretroviral therapy exposure	1188:1226	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	2	66	theme	baseline	465:472	arg1	DXA					519:521	DXA	519:521	DXA	519:521	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	66	theme	baseline	465:472	arg1	absorptiometry					503:516	baseline whole-body dual energy x-ray absorptiometry	465:516	baseline whole-body dual energy x-ray absorptiometry (DXA)	465:522	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	67	theme	[LBP	722:725	arg1	binding					706:712	lipopolysaccharide binding	687:712	lipopolysaccharide binding protein [LBP	687:725	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	1	68	theme	fat	193:195	arg1	composition					197:207	body fat composition	188:207	body fat composition	188:207	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	3	69	theme	intestinal	742:751	arg1	protein					772:778	intestinal fatty acid binding protein	742:778	intestinal fatty acid binding protein [I-FABP])	742:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	7	70	theme	interquartile	1363:1375	arg1	range					1377:1381	interquartile range	1363:1381	interquartile range	1363:1381	Median age at first DXA was 12 years (interquartile range, 10-14 years), 49% were female, and 69% were Black.
35090002	7	70	theme	interquartile	1363:1375	arg1	years					1390:1394	10-14 years	1384:1394	10-14 years	1384:1394	Median age at first DXA was 12 years (interquartile range, 10-14 years), 49% were female, and 69% were Black.
35090002	10	71	theme	gut	1833:1835	arg1	damage					1837:1842	gut damage	1833:1842	gut damage	1833:1842	CONCLUSIONS Despite viral suppression, gut damage and the resultant bacterial translocation are associated with body composition measures in YPHIV.
35090002	6	72	theme	second	1313:1318	arg1	DXA					1320:1322	a second DXA	1311:1322	a second DXA	1311:1322	RESULTS Two hundred sixty-one youth were included; 128 had a second DXA.
35090002	5	73	theme	baseline	1076:1083	arg1	log10					1098:1102	log10	1098:1102	log10	1098:1102	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	5	73	theme	baseline	1076:1083	arg1	markers					1089:1095	baseline gut markers	1076:1095	baseline gut markers (log10)	1076:1103	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	4	74	theme	truncal	927:933	arg1	body					921:924	percentage total body, truncal, and extremity fat	904:952	body	921:924	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	3	75	theme	protein	772:778	arg1	markers					623:629	Plasma markers	616:629	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP])	616:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	9	76	dep	associated	1683:1692	arg1	β = -2.36					1749:1757	β = -2.36	1749:1757	β = -2.36	1749:1757	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	9	76	dep	associated	1683:1692	arg1	-3.01					1763:1767	-3.01	1763:1767	-3.01	1763:1767	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	5	77	theme	markers	1089:1095	arg1	associations					1060:1071	associations	1060:1071	associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity	1060:1249	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	2	78	theme	baseline	571:578	arg1	DXA					580:582	baseline DXA	571:582	baseline DXA	571:582	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	79	link	enzyme-linked	819:831	arg1	assay					847:851	enzyme-linked immunosorbent assay	819:851	enzyme-linked immunosorbent assay	819:851	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	3	80	theme	acid	759:762	arg1	protein					772:778	intestinal fatty acid binding protein	742:778	intestinal fatty acid binding protein [I-FABP])	742:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	4	81	theme	percentage	904:913	arg1	body					921:924	percentage total body, truncal, and extremity fat	904:952	body	921:924	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	7	82	theme	first	1339:1343	arg1	DXA					1345:1347	first DXA	1339:1347	first DXA	1339:1347	Median age at first DXA was 12 years (interquartile range, 10-14 years), 49% were female, and 69% were Black.
35090002	10	83	theme	body	1906:1909	arg1	measures					1923:1930	body composition measures	1906:1930	body composition measures in YPHIV	1906:1939	CONCLUSIONS Despite viral suppression, gut damage and the resultant bacterial translocation are associated with body composition measures in YPHIV.
35090002	3	84	theme	immunosorbent	833:845	arg1	assay					847:851	enzyme-linked immunosorbent assay	819:851	enzyme-linked immunosorbent assay	819:851	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	10	85	theme	bacterial	1862:1870	arg1	translocation					1872:1884	the resultant bacterial translocation	1848:1884	the resultant bacterial translocation	1848:1884	CONCLUSIONS Despite viral suppression, gut damage and the resultant bacterial translocation are associated with body composition measures in YPHIV.
35090002	1	86	theme	gut	168:170	arg1	dysfunction					172:182	gut dysfunction	168:182	gut dysfunction	168:182	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	2	87	theme	x-ray	497:501	arg1	DXA					519:521	DXA	519:521	DXA	519:521	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	87	theme	x-ray	497:501	arg1	absorptiometry					503:516	baseline whole-body dual energy x-ray absorptiometry	465:516	baseline whole-body dual energy x-ray absorptiometry (DXA)	465:522	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	0	88	theme	Acquired	94:101	arg1	Virus					126:130	Perinatally Acquired Human Immunodeficiency Virus	82:130	Perinatally Acquired Human Immunodeficiency Virus	82:130	Gut Dysfunction Markers Are Associated With Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus.
35090002	8	89	dep	baseline	1549:1556	arg1	P < .01					1569:1575	P < .01	1569:1575	P < .01	1569:1575	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	8	89	dep	baseline	1549:1556	arg1	β = 4.08					1559:1566	β = 4.08	1559:1566	β = 4.08	1559:1566	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	6	90	contain	had	1307:1309	arg1	128					1303:1305	128	1303:1305	128	1303:1305	RESULTS Two hundred sixty-one youth were included; 128 had a second DXA.
35090002	6	90	contain	had	1307:1309	arg2	DXA					1320:1322	a second DXA	1311:1322	a second DXA	1311:1322	RESULTS Two hundred sixty-one youth were included; 128 had a second DXA.
35090002	2	91	theme	HIV	528:530	arg1	copies/mL					542:550	HIV RNA ≤1000 copies/mL	528:550	HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	528:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	0	92	from	Composition	49:59	arg1	Youth					64:68	Youth	64:68	Youth Living With Perinatally Acquired Human Immunodeficiency Virus	64:130	Gut Dysfunction Markers Are Associated With Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus.
35090002	0	93	theme	Immunodeficiency	109:124	arg1	Virus					126:130	Perinatally Acquired Human Immunodeficiency Virus	82:130	Perinatally Acquired Human Immunodeficiency Virus	82:130	Gut Dysfunction Markers Are Associated With Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus.
35090002	1	94	theme	human	240:244	arg1	virus					263:267	human immunodeficiency virus	240:267	perinatal human immunodeficiency virus infection (YPHIV)	230:285	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	2	95	theme	Adolescent	397:406	arg1	Protocol					415:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol	361:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma	361:434	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	0	96	theme	Dysfunction	4:14	arg1	Markers					16:22	Gut Dysfunction Markers	0:22	Gut Dysfunction Markers	0:22	Gut Dysfunction Markers Are Associated With Body Composition in Youth Living With Perinatally Acquired Human Immunodeficiency Virus.
35090002	3	97	dep	protein	772:778	arg1	[I-FABP					780:786	[I-FABP	780:786	[I-FABP	780:786	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	4	98	theme	extremity	940:948	arg1	fat					950:952	percentage total body, truncal, and extremity fat	904:952	fat	950:952	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	9	99	theme	total	1710:1714	arg1	fat					1721:1723	percentage total body fat	1699:1723	percentage total body fat at baseline and year 2	1699:1746	I-FABP was inversely associated with percentage total body fat at baseline and year 2 (β = -2.36 and -3.01, respectively, P ≤ .02).
35090002	8	100	theme	log10	1479:1483	arg1	LBP					1485:1487	log10 LBP	1479:1487	log10 LBP	1479:1487	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	2	101	theme	Cohort	384:389	arg1	Protocol					415:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol	361:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma	361:434	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	102	theme	dual	485:488	arg1	DXA					519:521	DXA	519:521	DXA	519:521	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	2	102	theme	dual	485:488	arg1	absorptiometry					503:516	baseline whole-body dual energy x-ray absorptiometry	465:516	baseline whole-body dual energy x-ray absorptiometry (DXA)	465:522	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	103	theme	Plasma	616:621	arg1	markers					623:629	Plasma markers	616:629	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP])	616:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	8	104	from	points	1627:1632	arg1	measures					1605:1612	adiposity measures	1595:1612	adiposity measures at both time points	1595:1632	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	5	105	theme	generalized	1017:1027	arg1	equations					1040:1048	generalized estimating equations	1017:1048	generalized estimating equations	1017:1048	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	2	106	theme	≤1000	536:540	arg1	copies/mL					542:550	HIV RNA ≤1000 copies/mL	528:550	HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	528:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	8	107	theme	percentage	1520:1529	arg1	fat					1542:1544	percentage total body fat	1520:1544	percentage total body fat at baseline (β = 4.08, P < .01) and zonulin	1520:1588	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	3	108	theme	gut	662:664	arg1	dysfunction					674:684	gut barrier dysfunction	662:684	gut barrier dysfunction	662:684	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	8	109	dep	associated	1504:1513	arg1	6.50					1646:1649	6.50	1646:1649	6.50	1646:1649	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	5	110	theme	Linear	977:982	arg1	models					995:1000	Linear regression models	977:1000	Linear regression models	977:1000	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
35090002	8	111	theme	body	1537:1540	arg1	fat					1542:1544	percentage total body fat	1520:1544	percentage total body fat at baseline (β = 4.08, P < .01) and zonulin	1520:1588	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	3	112	theme	translocation	644:656	arg1	markers					623:629	Plasma markers	616:629	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP])	616:788	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	4	113	from	DXA	972:974	arg1	fat					950:952	percentage total body, truncal, and extremity fat	904:952	fat	950:952	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	4	113	from	DXA	972:974	arg1	kilograms					957:965	kilograms	957:965	kilograms from DXA	957:974	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	4	113	from	DXA	972:974	arg1	body					921:924	percentage total body, truncal, and extremity fat	904:952	body	921:924	Adiposity outcomes included percentage total body, truncal, and extremity fat in kilograms from DXA.
35090002	2	114	from	Protocol	415:422	arg1	years					350:354	7-19 years	345:354	7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later	345:613	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	3	115	theme	lipopolysaccharide	687:704	arg1	binding					706:712	lipopolysaccharide binding	687:712	lipopolysaccharide binding protein [LBP	687:725	Plasma markers of bacterial translocation and gut barrier dysfunction (lipopolysaccharide binding protein [LBP], zonulin, and intestinal fatty acid binding protein [I-FABP]) were measured at baseline by enzyme-linked immunosorbent assay and log10 transformed.
35090002	1	116	theme	virus	263:267	arg1	YPHIV					280:284	YPHIV	280:284	YPHIV	280:284	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	1	116	theme	virus	263:267	arg1	infection					269:277	perinatal human immunodeficiency virus infection	230:277	perinatal human immunodeficiency virus infection (YPHIV)	230:285	BACKGROUND The association between gut dysfunction and body fat composition in youth living with perinatal human immunodeficiency virus infection (YPHIV) has not been investigated.
35090002	2	117	theme	Pediatric	365:373	arg1	Protocol					415:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol	361:422	the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma	361:434	METHODS We included YPHIV aged 7-19 years from the Pediatric HIV/AIDS Cohort Study Adolescent Master Protocol with plasma available within 6 months of baseline whole-body dual energy x-ray absorptiometry (DXA) and HIV RNA ≤1000 copies/mL within 3 months of baseline DXA and a second DXA 2 years later.
35090002	8	118	theme	adiposity	1595:1603	arg1	measures					1605:1612	adiposity measures	1595:1612	adiposity measures at both time points	1595:1632	After adjustment for potential confounders, log10 LBP was positively associated with percentage total body fat at baseline (β = 4.08, P < .01) and zonulin with adiposity measures at both time points (β = .94 to 6.50, P ≤ .01).
35090002	5	119	theme	physical	1233:1240	arg1	activity					1242:1249	physical activity	1233:1249	physical activity	1233:1249	Linear regression models were fit using generalized estimating equations to assess associations of baseline gut markers (log10) on adiposity outcomes at baseline and 2 years, adjusted for demographic variables, current antiretroviral therapy exposure, and physical activity.
34923905	0	0	theme	pilot	72:76	arg1	study					78:82	a pilot study	70:82	a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients	70:167	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	3	1	theme	maltodextrin	722:733	arg1	supplementation					741:755	maltodextrin daily supplementation	722:755	maltodextrin daily supplementation	722:755	A randomized, double-blind, placebo-controlled study included 50 AUD patients assigned to inulin versus maltodextrin daily supplementation for 17 days.
34923905	9	2	theme	psychological	1706:1718	arg1	parameters					1735:1744	other psychological and biological parameters as compared to placebo	1700:1767	other psychological and biological parameters as compared to placebo	1700:1767	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	9	3	from	microbiota	1622:1631	arg1	patients					1664:1671	AUD patients	1660:1671	AUD patients	1660:1671	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	1	4	theme	relapsing	210:218	arg1	disease					220:226	a chronic relapsing disease	200:226	a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms	200:375	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	1	4	theme	relapsing	210:218	arg1	disorder					182:189	Alcohol use disorder	170:189	Alcohol use disorder (AUD)	170:195	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	0	5	theme	disorder	151:158	arg1	patients					160:167	alcohol use disorder patients	139:167	alcohol use disorder patients	139:167	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	3	6	theme	randomized	620:629	arg1	study					665:669	A randomized, double-blind, placebo-controlled study	618:669	A randomized, double-blind, placebo-controlled study	618:669	A randomized, double-blind, placebo-controlled study included 50 AUD patients assigned to inulin versus maltodextrin daily supplementation for 17 days.
34923905	2	7	theme	microbiota	501:510	arg1	composition					512:522	the gut microbiota composition	493:522	the gut microbiota composition	493:522	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	4	8	theme	psychological	874:886	arg1	questionnaires					888:901	validated psychological questionnaires	864:901	validated psychological questionnaires	864:901	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	8	9	theme	only	1397:1400	arg1	patients					1402:1409	only patients	1397:1409	only patients treated with inulin	1397:1429	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	8	10	theme	increased	1487:1495	arg1	level					1503:1507	an increased serum level	1484:1507	an increased serum level of brain-derived neurotrophic factor	1484:1544	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	2	11	theme	gut	497:499	arg1	composition					512:522	the gut microbiota composition	493:522	the gut microbiota composition	493:522	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	4	12	theme	validated	864:872	arg1	questionnaires					888:901	validated psychological questionnaires	864:901	validated psychological questionnaires	864:901	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	10	13	dep	clinicaltrial.gov	1787:1803	arg1	//clinicaltrials.gov/ct2/show/NCT03803709					1825:1865	//clinicaltrials.gov/ct2/show/NCT03803709	1825:1865	//clinicaltrials.gov/ct2/show/NCT03803709	1825:1865	Gut2Brain study, clinicaltrial.gov: NCT03803709, https://clinicaltrials.gov/ct2/show/NCT03803709.
34923905	10	13	dep	clinicaltrial.gov	1787:1803	arg1	https					1819:1823	https	1819:1823	https	1819:1823	Gut2Brain study, clinicaltrial.gov: NCT03803709, https://clinicaltrials.gov/ct2/show/NCT03803709.
34923905	0	14	from	sociability	124:134	arg1	patients					160:167	alcohol use disorder patients	139:167	alcohol use disorder patients	139:167	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	9	15	theme	gut	1618:1620	arg1	microbiota					1622:1631	the gut microbiota	1614:1631	the gut microbiota	1614:1631	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	2	16	from	effect	544:549	arg1	metabolism					582:591	metabolism	582:591	metabolism	582:591	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	2	16	from	effect	544:549	arg1	tolerance					571:579	gastrointestinal tolerance	554:579	gastrointestinal tolerance	554:579	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	2	16	from	effect	544:549	arg1	behavior					608:615	patient's behavior	598:615	patient's behavior	598:615	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	4	17	theme	Biological	770:779	arg1	measurements					781:792	Biological measurements	770:792	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology)	770:845	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	8	18	link	brain-derived	1512:1524	arg1	factor					1539:1544	brain-derived neurotrophic factor	1512:1544	brain-derived neurotrophic factor	1512:1544	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	2	19	theme	prebiotic	429:437	arg1	fiber					439:443	prebiotic fiber	429:443	prebiotic fiber	429:443	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	8	20	contain	had	1480:1482	arg1	patients					1402:1409	only patients	1397:1409	only patients treated with inulin	1397:1429	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	8	20	contain	had	1480:1482	arg2	level					1503:1507	an increased serum level	1484:1507	an increased serum level of brain-derived neurotrophic factor	1484:1544	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	4	21	theme	dietary	848:854	arg1	intake					856:861	dietary intake	848:861	dietary intake	848:861	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	6	22	theme	nutritional	1199:1209	arg1	intakes					1211:1217	nutritional intakes	1199:1217	nutritional intakes	1199:1217	Prebiotic had minor effects on gastrointestinal symptoms and nutritional intakes compared to placebo.
34923905	7	23	theme	intervention	1362:1373	arg1	group					1375:1379	the intervention group	1358:1379	the intervention group	1358:1379	All patients showed an improvement in depression, anxiety, and craving scores during alcohol withdrawal regardless of the intervention group.
34923905	1	24	theme	Alcohol	170:176	arg1	disorder					182:189	Alcohol use disorder	170:189	Alcohol use disorder (AUD)	170:195	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	1	24	theme	Alcohol	170:176	arg1	disease					220:226	a chronic relapsing disease	200:226	a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms	200:375	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	1	24	theme	Alcohol	170:176	arg1	AUD					192:194	AUD	192:194	AUD	192:194	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	0	25	theme	dietary	22:28	arg1	intake					36:41	an adequate dietary fiber intake	10:41	an adequate dietary fiber intake by inulin supplementation	10:67	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	8	26	theme	serum	1497:1501	arg1	level					1503:1507	an increased serum level	1484:1507	an increased serum level of brain-derived neurotrophic factor	1484:1544	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	0	27	theme	gut	105:107	arg1	microbiota					109:118	gut microbiota	105:118	gut microbiota	105:118	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	6	28	contain	had	1148:1150	arg2	effects					1158:1164	minor effects	1152:1164	minor effects	1152:1164	Prebiotic had minor effects on gastrointestinal symptoms and nutritional intakes compared to placebo.
34923905	6	28	contain	had	1148:1150	arg1	Prebiotic					1138:1146	Prebiotic	1138:1146	Prebiotic	1138:1146	Prebiotic had minor effects on gastrointestinal symptoms and nutritional intakes compared to placebo.
34923905	8	29	theme	sociability	1458:1468	arg1	score					1470:1474	the sociability score	1454:1474	the sociability score	1454:1474	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	1	30	theme	use	178:180	arg1	disorder					182:189	Alcohol use disorder	170:189	Alcohol use disorder (AUD)	170:195	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	1	30	theme	use	178:180	arg1	disease					220:226	a chronic relapsing disease	200:226	a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms	200:375	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	1	30	theme	use	178:180	arg1	AUD					192:194	AUD	192:194	AUD	192:194	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	0	31	theme	adequate	13:20	arg1	intake					36:41	an adequate dietary fiber intake	10:41	an adequate dietary fiber intake by inulin supplementation	10:67	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	1	32	theme	metabolic	258:266	arg1	disturbances					268:279	metabolic disturbances	258:279	metabolic disturbances	258:279	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	4	33	theme	tolerance	925:933	arg1	assessment					935:944	gastrointestinal tolerance assessment	908:944	gastrointestinal tolerance assessment	908:944	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	1	34	theme	psychological	354:366	arg1	symptoms					368:375	psychological symptoms	354:375	psychological symptoms	354:375	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	6	35	theme	gastrointestinal	1169:1184	arg1	symptoms					1186:1193	gastrointestinal symptoms	1169:1193	gastrointestinal symptoms	1169:1193	Prebiotic had minor effects on gastrointestinal symptoms and nutritional intakes compared to placebo.
34923905	9	36	theme	biological	1724:1733	arg1	parameters					1735:1744	other psychological and biological parameters as compared to placebo	1700:1767	other psychological and biological parameters as compared to placebo	1700:1767	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	3	37	theme	daily	735:739	arg1	supplementation					741:755	maltodextrin daily supplementation	722:755	maltodextrin daily supplementation	722:755	A randomized, double-blind, placebo-controlled study included 50 AUD patients assigned to inulin versus maltodextrin daily supplementation for 17 days.
34923905	1	38	theme	symptoms	368:375	arg1	severity					342:349	the severity	338:349	the severity of psychological symptoms	338:375	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	4	39	theme	microbial	801:809	arg1	sequencing					820:829	fecal microbial 16S rDNA sequencing	795:829	fecal microbial 16S rDNA sequencing	795:829	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	4	39	theme	microbial	801:809	arg1	biology					838:844	serum biology	832:844	serum biology	832:844	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	0	40	theme	fiber	30:34	arg1	intake					36:41	an adequate dietary fiber intake	10:41	an adequate dietary fiber intake by inulin supplementation	10:67	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	2	41	theme	alcohol	452:458	arg1	withdrawal					460:469	alcohol withdrawal	452:469	alcohol withdrawal	452:469	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	8	42	theme	neurotrophic	1526:1537	arg1	factor					1539:1544	brain-derived neurotrophic factor	1512:1544	brain-derived neurotrophic factor	1512:1544	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	4	43	theme	rDNA	815:818	arg1	sequencing					820:829	fecal microbial 16S rDNA sequencing	795:829	fecal microbial 16S rDNA sequencing	795:829	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	4	43	theme	rDNA	815:818	arg1	biology					838:844	serum biology	832:844	serum biology	832:844	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	2	44	with	patients	415:422	arg1	fiber					439:443	prebiotic fiber	429:443	prebiotic fiber	429:443	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	9	45	theme	pilot	1552:1556	arg1	study					1558:1562	This pilot study	1547:1562	This pilot study	1547:1562	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	0	46	theme	inulin	46:51	arg1	supplementation					53:67	inulin supplementation	46:67	inulin supplementation	46:67	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	2	47	theme	gastrointestinal	554:569	arg1	tolerance					571:579	gastrointestinal tolerance	554:579	gastrointestinal tolerance	554:579	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	8	48	theme	brain-derived	1512:1524	arg1	factor					1539:1544	brain-derived neurotrophic factor	1512:1544	brain-derived neurotrophic factor	1512:1544	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	4	49	theme	serum	832:836	arg1	sequencing					820:829	fecal microbial 16S rDNA sequencing	795:829	fecal microbial 16S rDNA sequencing	795:829	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	4	49	theme	serum	832:836	arg1	biology					838:844	serum biology	832:844	serum biology	832:844	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	4	50	theme	gastrointestinal	908:923	arg1	assessment					935:944	gastrointestinal tolerance assessment	908:944	gastrointestinal tolerance assessment	908:944	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	1	51	theme	gut	286:288	arg1	alterations					301:311	gut microbiota alterations	286:311	gut microbiota alterations	286:311	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	0	52	from	impact	95:100	arg1	microbiota					109:118	gut microbiota	105:118	gut microbiota	105:118	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	0	52	from	impact	95:100	arg1	sociability					124:134	sociability	124:134	sociability	124:134	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	8	53	theme	factor	1539:1544	arg1	level					1503:1507	an increased serum level	1484:1507	an increased serum level of brain-derived neurotrophic factor	1484:1544	Interestingly, only patients treated with inulin significantly improved the sociability score and had an increased serum level of brain-derived neurotrophic factor.
34923905	7	54	theme	alcohol	1325:1331	arg1	withdrawal					1333:1342	alcohol withdrawal	1325:1342	alcohol withdrawal	1325:1342	All patients showed an improvement in depression, anxiety, and craving scores during alcohol withdrawal regardless of the intervention group.
34923905	1	55	theme	microbiota	290:299	arg1	alterations					301:311	gut microbiota alterations	286:311	gut microbiota alterations	286:311	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	0	56	theme	use	147:149	arg1	disorder					151:158	alcohol use disorder	139:158	alcohol use disorder patients	139:167	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	7	57	from	improvement	1263:1273	arg1	scores					1311:1316	craving scores	1303:1316	craving scores	1303:1316	All patients showed an improvement in depression, anxiety, and craving scores during alcohol withdrawal regardless of the intervention group.
34923905	7	57	from	improvement	1263:1273	arg1	anxiety					1290:1296	anxiety	1290:1296	anxiety	1290:1296	All patients showed an improvement in depression, anxiety, and craving scores during alcohol withdrawal regardless of the intervention group.
34923905	7	57	from	improvement	1263:1273	arg1	depression					1278:1287	depression	1278:1287	depression	1278:1287	All patients showed an improvement in depression, anxiety, and craving scores during alcohol withdrawal regardless of the intervention group.
34923905	4	58	theme	fecal	795:799	arg1	sequencing					820:829	fecal microbial 16S rDNA sequencing	795:829	fecal microbial 16S rDNA sequencing	795:829	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	4	58	theme	fecal	795:799	arg1	biology					838:844	serum biology	832:844	serum biology	832:844	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	9	59	theme	AUD	1660:1662	arg1	patients					1664:1671	AUD patients	1660:1671	AUD patients	1660:1671	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	6	60	theme	minor	1152:1156	arg1	effects					1158:1164	minor effects	1152:1164	minor effects	1152:1164	Prebiotic had minor effects on gastrointestinal symptoms and nutritional intakes compared to placebo.
34923905	9	61	theme	social	1641:1646	arg1	behavior					1648:1655	the social behavior	1637:1655	the social behavior in AUD patients	1637:1671	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	0	62	theme	alcohol	139:145	arg1	disorder					151:158	alcohol use disorder	139:158	alcohol use disorder patients	139:167	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	5	63	theme	genera	1078:1083	arg1	Bifidobacterium					1105:1119	Bifidobacterium	1105:1119	Bifidobacterium	1105:1119	Inulin significantly decreased the richness and evenness and induced changes of 8 genera (q < 0.1) including Bifidobacterium and Bacteroides.
34923905	5	63	theme	genera	1078:1083	arg1	Bacteroides					1125:1135	Bacteroides	1125:1135	Bacteroides	1125:1135	Inulin significantly decreased the richness and evenness and induced changes of 8 genera (q < 0.1) including Bifidobacterium and Bacteroides.
34923905	5	63	theme	genera	1078:1083	arg1	changes					1065:1071	changes	1065:1071	changes of 8 genera (q < 0.1) including Bifidobacterium and Bacteroides	1065:1135	Inulin significantly decreased the richness and evenness and induced changes of 8 genera (q < 0.1) including Bifidobacterium and Bacteroides.
34923905	9	64	theme	other	1700:1704	arg1	parameters					1735:1744	other psychological and biological parameters as compared to placebo	1700:1767	other psychological and biological parameters as compared to placebo	1700:1767	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	0	65	from	microbiota	109:118	arg1	patients					160:167	alcohol use disorder patients	139:167	alcohol use disorder patients	139:167	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	4	66	theme	16S	811:813	arg1	sequencing					820:829	fecal microbial 16S rDNA sequencing	795:829	fecal microbial 16S rDNA sequencing	795:829	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	4	66	theme	16S	811:813	arg1	biology					838:844	serum biology	832:844	serum biology	832:844	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	2	67	theme	AUD	411:413	arg1	patients					415:422	AUD patients	411:422	AUD patients with prebiotic fiber	411:443	This study aims at supplementing AUD patients with prebiotic fiber during alcohol withdrawal, in order to modulate the gut microbiota composition and to evaluate its effect on gastrointestinal tolerance, metabolism, and patient's behavior.
34923905	4	68	dep	measurements	781:792	arg1	sequencing					820:829	fecal microbial 16S rDNA sequencing	795:829	fecal microbial 16S rDNA sequencing	795:829	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	4	68	dep	measurements	781:792	arg1	biology					838:844	serum biology	832:844	serum biology	832:844	Biological measurements (fecal microbial 16S rDNA sequencing, serum biology), dietary intake, validated psychological questionnaires, and gastrointestinal tolerance assessment were performed before and after the intervention.
34923905	9	69	from	behavior	1648:1655	arg1	patients					1664:1671	AUD patients	1660:1671	AUD patients	1660:1671	This pilot study shows that inulin is well tolerated and modulates the gut microbiota and the social behavior in AUD patients, without further improving other psychological and biological parameters as compared to placebo.
34923905	3	70	dep	randomized	620:629	arg1	placebo-controlled					646:663	placebo-controlled	646:663	placebo-controlled	646:663	A randomized, double-blind, placebo-controlled study included 50 AUD patients assigned to inulin versus maltodextrin daily supplementation for 17 days.
34923905	3	70	dep	randomized	620:629	arg1	double-blind					632:643	double-blind	632:643	double-blind	632:643	A randomized, double-blind, placebo-controlled study included 50 AUD patients assigned to inulin versus maltodextrin daily supplementation for 17 days.
34923905	3	71	theme	AUD	683:685	arg1	patients					687:694	50 AUD patients	680:694	50 AUD patients assigned to inulin versus maltodextrin daily supplementation for 17 days	680:767	A randomized, double-blind, placebo-controlled study included 50 AUD patients assigned to inulin versus maltodextrin daily supplementation for 17 days.
34923905	0	72	dep	Restoring	0:8	arg1	study					78:82	a pilot study	70:82	a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients	70:167	Restoring an adequate dietary fiber intake by inulin supplementation: a pilot study showing an impact on gut microbiota and sociability in alcohol use disorder patients.
34923905	7	73	theme	craving	1303:1309	arg1	scores					1311:1316	craving scores	1303:1316	craving scores	1303:1316	All patients showed an improvement in depression, anxiety, and craving scores during alcohol withdrawal regardless of the intervention group.
34923905	1	74	theme	chronic	202:208	arg1	disease					220:226	a chronic relapsing disease	200:226	a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms	200:375	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
34923905	1	74	theme	chronic	202:208	arg1	disorder					182:189	Alcohol use disorder	170:189	Alcohol use disorder (AUD)	170:195	Alcohol use disorder (AUD) is a chronic relapsing disease associated with malnutrition, metabolic disturbances, and gut microbiota alterations that are correlated with the severity of psychological symptoms.
35185872	12	0	theme	intestinal	1944:1953	arg1	composition					1966:1976	the intestinal microbiota composition	1940:1976	the intestinal microbiota composition	1940:1976	Furthermore, IAA altered the composition of the intestinal microbiota composition by increasing Bacteroides and decreasing Proteobacteria and Firmicutes, in addition to increasing the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri.
35185872	7	1	theme	IAA	842:844	arg1	effects					831:837	The effects	827:837	The effects of IAA on AS mice	827:855	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	9	2	from	severity	1190:1197	arg1	mice					1208:1211	mice	1208:1211	mice	1208:1211	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	7	3	theme	cytokine	1038:1045	arg1	levels					1047:1052	inflammatory cytokine levels	1025:1052	inflammatory cytokine levels	1025:1052	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	8	4	theme	whole-genome	1116:1127	arg1	sequencing					1129:1138	whole-genome sequencing	1116:1138	whole-genome sequencing	1116:1138	The intestinal microbiota compositions were profiled through whole-genome sequencing.
35185872	14	5	theme	therapeutic	2414:2424	arg1	approach					2426:2433	a novel therapeutic approach	2406:2433	a novel therapeutic approach for AS	2406:2440	IAA might represent a novel therapeutic approach for AS.
35185872	1	6	theme	autoimmune	166:175	arg1	disease					177:183	a systemic, chronic, and inflammatory autoimmune disease	128:183	a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota	128:237	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	1	6	theme	autoimmune	166:175	arg1	spondylitis					108:118	Ankylosing spondylitis	97:118	Ankylosing spondylitis (AS)	97:123	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	6	7	theme	mg/kg	760:764	arg1	weight					771:776	50 mg/kg body weight	757:776	50 mg/kg body weight	757:776	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	6	7	theme	mg/kg	760:764	arg1	IAA					752:754	administered IAA	739:754	administered IAA (50 mg/kg body weight)	739:777	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	13	8	from	mice	2232:2235	arg1	PG-induced					2215:2224	PG-induced	2215:2224	PG-induced	2215:2224	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	7	9	theme	Treg	1006:1009	arg1	balance					1012:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	10	10	theme	barrier	1551:1557	arg1	function					1559:1566	intestinal mucosal barrier function	1532:1566	intestinal mucosal barrier function	1532:1566	IAA ameliorated pathological changes in the ileum and improved intestinal mucosal barrier function.
35185872	11	11	theme	acid	1753:1756	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	11	theme	acid	1753:1756	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	11	theme	acid	1753:1756	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	7	12	theme	regulatory	994:1003	arg1	balance					1012:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	9	13	theme	cytokine	1392:1399	arg1	IL-10					1401:1405	the anti-inflammatory cytokine IL-10	1370:1405	the anti-inflammatory cytokine IL-10	1370:1405	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	13	14	theme	several	2180:2186	arg1	effects					2199:2205	several protective effects	2180:2205	several protective effects	2180:2205	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	10	15	theme	intestinal	1532:1541	arg1	function					1559:1566	intestinal mucosal barrier function	1532:1566	intestinal mucosal barrier function	1532:1566	IAA ameliorated pathological changes in the ileum and improved intestinal mucosal barrier function.
35185872	9	16	theme	AS	1202:1203	arg1	severity					1190:1197	severity	1190:1197	severity	1190:1197	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	16	theme	AS	1202:1203	arg1	incidence					1176:1184	incidence	1176:1184	incidence	1176:1184	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	3	17	theme	Recent	295:300	arg1	evidence					302:309	Recent evidence	295:309	Recent evidence	295:309	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	1	18	theme	systemic	130:137	arg1	disease					177:183	a systemic, chronic, and inflammatory autoimmune disease	128:183	a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota	128:237	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	1	18	theme	systemic	130:137	arg1	spondylitis					108:118	Ankylosing spondylitis	97:118	Ankylosing spondylitis (AS)	97:123	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	12	19	theme	composition	1966:1976	arg1	composition					1925:1935	the composition	1921:1935	the composition of the intestinal microbiota composition	1921:1976	Furthermore, IAA altered the composition of the intestinal microbiota composition by increasing Bacteroides and decreasing Proteobacteria and Firmicutes, in addition to increasing the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri.
35185872	7	20	theme	hydrocarbon	937:947	arg1	AhR					959:961	AhR	959:961	AhR	959:961	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	7	20	theme	hydrocarbon	937:947	arg1	receptor					949:956	aryl hydrocarbon receptor	932:956	aryl hydrocarbon receptor (AhR) pathway	932:970	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	1	21	theme	chronic	140:146	arg1	disease					177:183	a systemic, chronic, and inflammatory autoimmune disease	128:183	a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota	128:237	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	1	21	theme	chronic	140:146	arg1	spondylitis					108:118	Ankylosing spondylitis	97:118	Ankylosing spondylitis (AS)	97:123	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	9	22	theme	pro-/anti-	1434:1443	arg1	cytokines					1458:1466	pro-/anti- inflammatory cytokines	1434:1466	pro-/anti- inflammatory cytokines	1434:1466	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	11	23	theme	increased	1678:1686	arg1	cells					1693:1697	increased Treg cells	1678:1697	increased Treg cells	1678:1697	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	9	24	theme	pro-inflammatory	1242:1257	arg1	cytokines					1259:1267	pro-inflammatory cytokines	1242:1267	pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23)	1242:1340	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	24	theme	pro-inflammatory	1242:1257	arg1	IL-17A					1323:1328	IL-17A	1323:1328	IL-17A	1323:1328	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	24	theme	pro-inflammatory	1242:1257	arg1	IL-23					1335:1339	IL-23	1335:1339	IL-23	1335:1339	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	24	theme	pro-inflammatory	1242:1257	arg1	[IL					1315:1317	interleukin [IL]-6	1303:1320	interleukin [IL]-6	1303:1320	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	7	25	theme	barrier	914:920	arg1	function					922:929	intestinal barrier function	903:929	intestinal barrier function	903:929	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	9	26	theme	cytokines	1458:1466	arg1	ratios					1424:1429	the ratios	1420:1429	the ratios of pro-/anti- inflammatory cytokines	1420:1466	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	27	from	incidence	1176:1184	arg1	mice					1208:1211	mice	1208:1211	mice	1208:1211	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	3	28	theme	tryptophan	381:390	arg1	acid					346:349	indole-3-acetic acid	330:349	indole-3-acetic acid (IAA)	330:355	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	3	28	theme	tryptophan	381:390	arg1	metabolite					392:401	an important microbial tryptophan metabolite	358:401	an important microbial tryptophan metabolite	358:401	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	11	29	theme	signal	1811:1816	arg1	transducer					1818:1827	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	transducer	1818:1827	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	29	theme	signal	1811:1816	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	0	30	theme	Indole-3-Acetic	0:14	arg1	Acid					16:19	Indole-3-Acetic Acid	0:19	Indole-3-Acetic Acid	0:19	Indole-3-Acetic Acid Alters Intestinal Microbiota and Alleviates Ankylosing Spondylitis in Mice.
35185872	9	31	theme	necrosis	1276:1283	arg1	factor					1285:1290	tumor necrosis factor α	1270:1292	tumor necrosis factor α [TNF-α	1270:1299	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	7	32	theme	disease	885:891	arg1	severity					893:900	disease severity	885:900	disease severity	885:900	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	3	33	theme	important	361:369	arg1	acid					346:349	indole-3-acetic acid	330:349	indole-3-acetic acid (IAA)	330:355	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	3	33	theme	important	361:369	arg1	metabolite					392:401	an important microbial tryptophan metabolite	358:401	an important microbial tryptophan metabolite	358:401	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	11	34	theme	Th17	1884:1887	arg1	cells					1889:1893	Th17 cells	1884:1893	Th17 cells	1884:1893	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	13	35	theme	protective	2188:2197	arg1	effects					2199:2205	several protective effects	2180:2205	several protective effects	2180:2205	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	11	36	theme	factor	1635:1640	arg1	FoxP3					1667:1671	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	37	theme	gamma	1791:1795	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	37	theme	gamma	1791:1795	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	37	theme	gamma	1791:1795	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	9	38	theme	interleukin	1303:1313	arg1	cytokines					1259:1267	pro-inflammatory cytokines	1242:1267	pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23)	1242:1340	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	38	theme	interleukin	1303:1313	arg1	[IL					1315:1317	interleukin [IL]-6	1303:1320	interleukin [IL]-6	1303:1320	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	39	dep	cytokines	1259:1267	arg1	cytokines					1259:1267	pro-inflammatory cytokines	1242:1267	pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23)	1242:1340	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	39	dep	cytokines	1259:1267	arg1	IL-17A					1323:1328	IL-17A	1323:1328	IL-17A	1323:1328	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	39	dep	cytokines	1259:1267	arg1	IL-23					1335:1339	IL-23	1335:1339	IL-23	1335:1339	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	39	dep	cytokines	1259:1267	arg1	factor					1285:1290	tumor necrosis factor α	1270:1292	tumor necrosis factor α [TNF-α	1270:1299	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	9	39	dep	cytokines	1259:1267	arg1	[IL					1315:1317	interleukin [IL]-6	1303:1320	interleukin [IL]-6	1303:1320	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	11	40	theme	P3	1663:1664	arg1	FoxP3					1667:1671	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	41	theme	orphan	1775:1780	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	41	theme	orphan	1775:1780	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	41	theme	orphan	1775:1780	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	1	42	theme	intestinal	217:226	arg1	microbiota					228:237	intestinal microbiota	217:237	intestinal microbiota	217:237	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	12	43	dep	increasing	2065:2074	arg1	addition					2053:2060	addition	2053:2060	addition	2053:2060	Furthermore, IAA altered the composition of the intestinal microbiota composition by increasing Bacteroides and decreasing Proteobacteria and Firmicutes, in addition to increasing the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri.
35185872	11	44	theme	box	1651:1653	arg1	FoxP3					1667:1671	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	7	45	from	effects	831:837	arg1	mice					852:855	AS mice	849:855	AS mice	849:855	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	6	46	theme	mouse	723:727	arg1	model					729:733	a proteoglycan (PG)-induced AS mouse model	692:733	a proteoglycan (PG)-induced AS mouse model	692:733	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	13	47	theme	balance	2279:2285	arg1	restoration					2264:2274	the restoration	2260:2274	the restoration of balance among the intestinal microbial community	2260:2326	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	6	48	theme	-induced	711:718	arg1	model					729:733	a proteoglycan (PG)-induced AS mouse model	692:733	a proteoglycan (PG)-induced AS mouse model	692:733	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	8	49	theme	microbiota	1070:1079	arg1	compositions					1081:1092	The intestinal microbiota compositions	1055:1092	The intestinal microbiota compositions	1055:1092	The intestinal microbiota compositions were profiled through whole-genome sequencing.
35185872	7	50	theme	inflammatory	1025:1036	arg1	levels					1047:1052	inflammatory cytokine levels	1025:1052	inflammatory cytokine levels	1025:1052	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	6	51	theme	intraperitoneal	782:796	arg1	injection					798:806	intraperitoneal injection	782:806	intraperitoneal injection daily for 4 weeks	782:824	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	10	52	theme	pathological	1485:1496	arg1	changes					1498:1504	pathological changes	1485:1504	pathological changes in the ileum	1485:1517	IAA ameliorated pathological changes in the ileum and improved intestinal mucosal barrier function.
35185872	5	53	theme	underlying	620:629	arg1	mechanisms					631:640	underlying mechanisms	620:640	underlying mechanisms through which IAA acts against AS	620:674	In this study, we investigated the protective effects and underlying mechanisms through which IAA acts against AS.
35185872	13	54	from	PG-induced	2215:2224	arg1	mice					2232:2235	mice	2232:2235	mice	2232:2235	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	1	55	theme	inflammatory	153:164	arg1	disease					177:183	a systemic, chronic, and inflammatory autoimmune disease	128:183	a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota	128:237	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	1	55	theme	inflammatory	153:164	arg1	spondylitis					108:118	Ankylosing spondylitis	97:118	Ankylosing spondylitis (AS)	97:123	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	14	56	theme	novel	2408:2412	arg1	approach					2426:2433	a novel therapeutic approach	2406:2433	a novel therapeutic approach for AS	2406:2440	IAA might represent a novel therapeutic approach for AS.
35185872	4	57	dep	in	516:517	arg1	vivo					519:522	vivo	519:522	vivo	519:522	However, reports have not examined the in vivo protective effects of IAA against AS.
35185872	11	58	theme	AhR	1592:1594	arg1	pathway					1596:1602	the AhR pathway	1588:1602	the AhR pathway	1588:1602	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	6	59	theme	body	766:769	arg1	weight					771:776	50 mg/kg body weight	757:776	50 mg/kg body weight	757:776	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	6	59	theme	body	766:769	arg1	IAA					752:754	administered IAA	739:754	administered IAA (50 mg/kg body weight)	739:777	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	13	60	dep	exerted	2172:2178	arg1	inhibiting					2361:2370	inhibiting	2361:2370	inhibiting inflammation	2361:2383	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	13	60	dep	exerted	2172:2178	arg1	activating					2329:2338	activating	2329:2338	activating the AhR pathway	2329:2354	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	11	61	theme	transcription	1722:1734	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	61	theme	transcription	1722:1734	arg1	activator					1833:1841	activator	1833:1841	activator	1833:1841	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	61	theme	transcription	1722:1734	arg1	transducer					1818:1827	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	transducer	1818:1827	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	61	theme	transcription	1722:1734	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	7	62	theme	/T	991:992	arg1	balance					1012:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	9	63	theme	anti-inflammatory	1374:1390	arg1	IL-10					1401:1405	the anti-inflammatory cytokine IL-10	1370:1405	the anti-inflammatory cytokine IL-10	1370:1405	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	4	64	theme	IAA	546:548	arg1	effects					535:541	the in vivo protective effects	512:541	the in vivo protective effects of IAA against AS	512:559	However, reports have not examined the in vivo protective effects of IAA against AS.
35185872	10	65	theme	mucosal	1543:1549	arg1	function					1559:1566	intestinal mucosal barrier function	1532:1566	intestinal mucosal barrier function	1532:1566	IAA ameliorated pathological changes in the ileum and improved intestinal mucosal barrier function.
35185872	11	66	theme	receptor-related	1758:1773	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	66	theme	receptor-related	1758:1773	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	66	theme	receptor-related	1758:1773	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	7	67	theme	Th17	986:989	arg1	balance					1012:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	7	68	theme	T-helper	973:980	arg1	balance					1012:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	T-helper 17 (Th17)/T regulatory (Treg) balance	973:1018	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	9	69	theme	IL-10	1401:1405	arg1	production					1356:1365	the production	1352:1365	the production of the anti-inflammatory cytokine IL-10	1352:1405	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	11	70	theme	retinoic	1744:1751	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	70	theme	retinoic	1744:1751	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	70	theme	retinoic	1744:1751	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	5	71	theme	protective	597:606	arg1	effects					608:614	the protective effects	593:614	the protective effects	593:614	In this study, we investigated the protective effects and underlying mechanisms through which IAA acts against AS.
35185872	2	72	theme	effective	255:263	arg1	therapies					265:273	effective therapies	255:273	effective therapies for AS	255:280	Unfortunately, effective therapies for AS are lacking.
35185872	4	73	theme	in	516:517	arg1	effects					535:541	the in vivo protective effects	512:541	the in vivo protective effects of IAA against AS	512:559	However, reports have not examined the in vivo protective effects of IAA against AS.
35185872	12	74	theme	microbiota	1955:1964	arg1	composition					1966:1976	the intestinal microbiota composition	1940:1976	the intestinal microbiota composition	1940:1976	Furthermore, IAA altered the composition of the intestinal microbiota composition by increasing Bacteroides and decreasing Proteobacteria and Firmicutes, in addition to increasing the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri.
35185872	7	75	theme	receptor	949:956	arg1	pathway					964:970	aryl hydrocarbon receptor (AhR) pathway	932:970	aryl hydrocarbon receptor (AhR) pathway	932:970	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	11	76	dep	factors	1736:1742	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	76	dep	factors	1736:1742	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	76	dep	factors	1736:1742	arg1	activator					1833:1841	activator	1833:1841	activator	1833:1841	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	76	dep	factors	1736:1742	arg1	transducer					1818:1827	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	transducer	1818:1827	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	76	dep	factors	1736:1742	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	3	77	theme	indole-3-acetic	330:344	arg1	acid					346:349	indole-3-acetic acid	330:349	indole-3-acetic acid (IAA)	330:355	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	3	77	theme	indole-3-acetic	330:344	arg1	metabolite					392:401	an important microbial tryptophan metabolite	358:401	an important microbial tryptophan metabolite	358:401	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	3	77	theme	indole-3-acetic	330:344	arg1	IAA					352:354	IAA	352:354	IAA	352:354	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	4	78	theme	protective	524:533	arg1	effects					535:541	the in vivo protective effects	512:541	the in vivo protective effects of IAA against AS	512:559	However, reports have not examined the in vivo protective effects of IAA against AS.
35185872	1	79	dep	spondylitis	108:118	arg1	AS					121:122	AS	121:122	AS	121:122	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	13	80	theme	microbial	2308:2316	arg1	community					2318:2326	the intestinal microbial community	2293:2326	the intestinal microbial community	2293:2326	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	7	81	theme	aryl	932:935	arg1	AhR					959:961	AhR	959:961	AhR	959:961	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	7	81	theme	aryl	932:935	arg1	receptor					949:956	aryl hydrocarbon receptor	932:956	aryl hydrocarbon receptor (AhR) pathway	932:970	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	9	82	theme	inflammatory	1445:1456	arg1	cytokines					1458:1466	pro-/anti- inflammatory cytokines	1434:1466	pro-/anti- inflammatory cytokines	1434:1466	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	11	83	theme	Treg	1688:1691	arg1	cells					1693:1697	increased Treg cells	1678:1697	increased Treg cells	1678:1697	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	9	84	theme	cytokines	1259:1267	arg1	production					1228:1237	the production	1224:1237	the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23)	1224:1340	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	3	85	theme	inflammatory	453:464	arg1	responses					466:474	inflammatory responses	453:474	inflammatory responses	453:474	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	9	86	theme	tumor	1270:1274	arg1	factor					1285:1290	tumor necrosis factor α	1270:1292	tumor necrosis factor α [TNF-α	1270:1299	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	7	87	theme	intestinal	903:912	arg1	function					922:929	intestinal barrier function	903:929	intestinal barrier function	903:929	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
35185872	1	88	theme	Ankylosing	97:106	arg1	disease					177:183	a systemic, chronic, and inflammatory autoimmune disease	128:183	a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota	128:237	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	1	88	theme	Ankylosing	97:106	arg1	spondylitis					108:118	Ankylosing spondylitis	97:118	Ankylosing spondylitis (AS)	97:123	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	6	89	theme	daily	808:812	arg1	injection					798:806	intraperitoneal injection	782:806	intraperitoneal injection daily for 4 weeks	782:824	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	0	90	theme	Intestinal	28:37	arg1	Microbiota					39:48	Intestinal Microbiota	28:48	Intestinal Microbiota	28:48	Indole-3-Acetic Acid Alters Intestinal Microbiota and Alleviates Ankylosing Spondylitis in Mice.
35185872	12	91	theme	pseudolongum	2110:2121	arg1	abundances					2080:2089	the abundances	2076:2089	the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri	2076:2150	Furthermore, IAA altered the composition of the intestinal microbiota composition by increasing Bacteroides and decreasing Proteobacteria and Firmicutes, in addition to increasing the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri.
35185872	3	92	theme	microbial	371:379	arg1	acid					346:349	indole-3-acetic acid	330:349	indole-3-acetic acid (IAA)	330:355	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	3	92	theme	microbial	371:379	arg1	metabolite					392:401	an important microbial tryptophan metabolite	358:401	an important microbial tryptophan metabolite	358:401	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	11	93	theme	transcription	1621:1633	arg1	FoxP3					1667:1671	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	9	94	theme	[TNF-α	1294:1299	arg1	factor					1285:1290	tumor necrosis factor α	1270:1292	tumor necrosis factor α [TNF-α	1270:1299	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	3	95	theme	intestinal	417:426	arg1	homeostasis					428:438	intestinal homeostasis	417:438	intestinal homeostasis	417:438	Recent evidence has indicated that indole-3-acetic acid (IAA), an important microbial tryptophan metabolite, can modulate intestinal homeostasis and suppress inflammatory responses.
35185872	11	96	theme	transcription	1846:1858	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	96	theme	transcription	1846:1858	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	96	theme	transcription	1846:1858	arg1	activator					1833:1841	activator	1833:1841	activator	1833:1841	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	96	theme	transcription	1846:1858	arg1	transducer					1818:1827	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	transducer	1818:1827	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	96	theme	transcription	1846:1858	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	13	97	theme	AhR	2344:2346	arg1	pathway					2348:2354	the AhR pathway	2340:2354	the AhR pathway	2340:2354	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	11	98	theme	protein	1655:1661	arg1	FoxP3					1667:1671	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	12	99	theme	schaedleri	2141:2150	arg1	abundances					2080:2089	the abundances	2076:2089	the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri	2076:2150	Furthermore, IAA altered the composition of the intestinal microbiota composition by increasing Bacteroides and decreasing Proteobacteria and Firmicutes, in addition to increasing the abundances of Bifidobacterium pseudolongum and Mucispirillum schaedleri.
35185872	6	100	theme	administered	739:750	arg1	weight					771:776	50 mg/kg body weight	757:776	50 mg/kg body weight	757:776	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	6	100	theme	administered	739:750	arg1	IAA					752:754	administered IAA	739:754	administered IAA (50 mg/kg body weight)	739:777	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	11	101	theme	t	1797:1797	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	101	theme	t	1797:1797	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	101	theme	t	1797:1797	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	13	102	theme	intestinal	2297:2306	arg1	community					2318:2326	the intestinal microbial community	2293:2326	the intestinal microbial community	2293:2326	In conclusion, IAA exerted several protective effects against PG-induced AS in mice, which was mediated by the restoration of balance among the intestinal microbial community, activating the AhR pathway, and inhibiting inflammation.
35185872	11	103	theme	forehead	1642:1649	arg1	FoxP3					1667:1671	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	the transcription factor forehead box protein P3 (FoxP3)	1617:1672	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	104	theme	receptor	1782:1789	arg1	RORγt					1800:1804	retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer	1744:1827	RORγt	1800:1804	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	104	theme	receptor	1782:1789	arg1	STAT3					1863:1867	STAT3	1863:1867	STAT3	1863:1867	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	11	104	theme	receptor	1782:1789	arg1	factors					1736:1742	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3)	1718:1868	IAA also activated the AhR pathway, upregulated the transcription factor forehead box protein P3 (FoxP3) and increased Treg cells, and downregulated the transcription factors retinoic acid receptor-related orphan receptor gamma t (RORγt) and signal transducer and activator of transcription 3 (STAT3) and decreased Th17 cells.
35185872	1	105	theme	microbiota	228:237	arg1	disorder					205:212	the disorder	201:212	the disorder of intestinal microbiota	201:237	Ankylosing spondylitis (AS) is a systemic, chronic, and inflammatory autoimmune disease associated with the disorder of intestinal microbiota.
35185872	8	106	theme	intestinal	1059:1068	arg1	compositions					1081:1092	The intestinal microbiota compositions	1055:1092	The intestinal microbiota compositions	1055:1092	The intestinal microbiota compositions were profiled through whole-genome sequencing.
35185872	10	107	from	changes	1498:1504	arg1	ileum					1513:1517	the ileum	1509:1517	the ileum	1509:1517	IAA ameliorated pathological changes in the ileum and improved intestinal mucosal barrier function.
35185872	9	108	dep	incidence	1176:1184	arg1	the					1172:1174	the	1172:1174	the	1172:1174	We observed that IAA decreased the incidence and severity of AS in mice, inhibited the production of pro-inflammatory cytokines (tumor necrosis factor α [TNF-α], interleukin [IL]-6, IL-17A, and IL-23), promoted the production of the anti-inflammatory cytokine IL-10, and reduced the ratios of pro-/anti- inflammatory cytokines.
35185872	6	109	theme	AS	720:721	arg1	model					729:733	a proteoglycan (PG)-induced AS mouse model	692:733	a proteoglycan (PG)-induced AS mouse model	692:733	We constructed a proteoglycan (PG)-induced AS mouse model and administered IAA (50 mg/kg body weight) by intraperitoneal injection daily for 4 weeks.
35185872	7	110	theme	AS	849:850	arg1	mice					852:855	AS mice	849:855	AS mice	849:855	The effects of IAA on AS mice were evaluated by examining disease severity, intestinal barrier function, aryl hydrocarbon receptor (AhR) pathway, T-helper 17 (Th17)/T regulatory (Treg) balance, and inflammatory cytokine levels.
36988616	2	0	theme	mouse	570:574	arg1	model					576:580	a mouse model	568:580	a mouse model of dextran sodium sulfate (DSS)-induced colitis	568:628	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	4	1	theme	microbial	976:984	arg1	composition					986:996	intestinal microbial composition	965:996	intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella	965:1168	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	5	2	theme	Lactobacillus	1403:1415	arg1	strains					1417:1423	two Lactobacillus strains	1399:1423	two Lactobacillus strains	1399:1423	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	1	3	theme	interaction	278:288	arg1	balance					261:267	the balance	257:267	the balance of their interaction with a susceptible host	257:312	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	4	4	theme	additional	825:834	arg1	effects					847:853	several additional beneficial effects	817:853	several additional beneficial effects	817:853	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	5	theme	close	998:1002	arg1	composition					986:996	intestinal microbial composition	965:996	intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella	965:1168	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	6	theme	pro-inflammatory	1247:1262	arg1	lipopolysaccharides					1264:1282	pro-inflammatory lipopolysaccharides	1247:1282	pro-inflammatory lipopolysaccharides	1247:1282	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	7	dep	altered	957:963	arg1	ii					953:954	ii	953:954	ii	953:954	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	5	8	theme	Combination	1372:1382	arg1	treatment					1384:1392	CONCLUSION Combination treatment	1361:1392	CONCLUSION Combination treatment with two Lactobacillus strains	1361:1423	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	3	9	theme	weight	723:728	arg1	loss					730:733	weight loss	723:733	weight loss	723:733	LS treatment suppressed weight loss and colon shortening, and reduced disease activity index in the mice.
36988616	2	10	theme	human	550:554	arg1	infants					556:562	healthy human infants	542:562	healthy human infants	542:562	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	4	11	theme	pathway	1352:1358	arg1	overactivation					1300:1313	overactivation	1300:1313	overactivation of TLR4/NF-κB inflammatory signaling pathway	1300:1358	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	0	12	theme	intestinal	121:130	arg1	composition					142:152	intestinal microbial composition	121:152	intestinal microbial composition	121:152	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	1	13	theme	intestinal	225:234	arg1	microbes					236:243	intestinal microbes	225:243	intestinal microbes	225:243	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	4	14	theme	goblet	871:876	arg1	numbers					883:889	goblet cell numbers	871:889	goblet cell numbers	871:889	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	15	theme	intestinal	965:974	arg1	composition					986:996	intestinal microbial composition	965:996	intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella	965:1168	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	2	16	theme	infants	556:562	arg1	feces					533:537	feces	533:537	feces of healthy human infants	533:562	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	17	theme	ameliorative	651:662	arg1	effect					664:669	their ameliorative effect	645:669	their ameliorative effect	645:669	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	4	18	theme	inflammatory	1329:1340	arg1	pathway					1352:1358	TLR4/NF-κB inflammatory signaling pathway	1318:1358	TLR4/NF-κB inflammatory signaling pathway	1318:1358	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	2	19	theme	healthy	542:548	arg1	infants					556:562	healthy human infants	542:562	healthy human infants	542:562	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	4	20	theme	barrier	918:924	arg1	damage					926:931	intestinal barrier damage	907:931	intestinal barrier damage in colonic tissue	907:949	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	2	21	theme	possible	679:686	arg1	mechanism					688:696	its possible mechanism	675:696	its possible mechanism	675:696	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	0	22	theme	colitis	75:81	arg1	symptoms					83:90	colitis symptoms	75:90	colitis symptoms	75:90	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	1	23	theme	bowel	356:360	arg1	IBD					371:373	IBD	371:373	IBD	371:373	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	1	23	theme	bowel	356:360	arg1	disease					362:368	inflammatory bowel disease	343:368	inflammatory bowel disease (IBD)	343:374	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	5	24	theme	mouse	1470:1474	arg1	model					1476:1480	our mouse model	1466:1480	our mouse model	1466:1480	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	4	25	dep	reduced	1228:1234	arg1	iv					1224:1225	iv	1224:1225	iv	1224:1225	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	3	26	theme	disease	769:775	arg1	index					786:790	disease activity index	769:790	disease activity index in the mice	769:802	LS treatment suppressed weight loss and colon shortening, and reduced disease activity index in the mice.
36988616	4	27	dep	maintained	860:869	arg1	i					857:857	i	857:857	i	857:857	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	5	28	theme	colitis	1446:1452	arg1	symptoms					1454:1461	colitis symptoms	1446:1461	colitis symptoms	1446:1461	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	2	29	theme	LS	492:493	arg1	"					504:504	"LS treatment"	491:504	"LS treatment"	491:504	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	29	theme	LS	492:493	arg1	strains					441:447	combination two Lactobacillus strains	411:447	combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment")	411:505	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	0	30	theme	Lactobacillus	0:12	arg1	treatment					53:61	Lactobacillus rhamnosus and L. plantarum combination treatment	0:61	Lactobacillus rhamnosus and L. plantarum combination treatment	0:61	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	1	31	theme	susceptible	297:307	arg1	host					309:312	a susceptible host	295:312	a susceptible host	295:312	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	2	32	theme	L.	450:451	arg1	BY-02					463:467	L. rhamnosus BY-02	450:467	L. rhamnosus BY-02	450:467	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	32	theme	L.	450:451	arg1	strains					441:447	combination two Lactobacillus strains	411:447	combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment")	411:505	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	33	theme	-induced	613:620	arg1	colitis					622:628	dextran sodium sulfate (DSS)-induced colitis	585:628	dextran sodium sulfate (DSS)-induced colitis	585:628	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	3	34	from	index	786:790	arg1	mice					799:802	the mice	795:802	the mice	795:802	LS treatment suppressed weight loss and colon shortening, and reduced disease activity index in the mice.
36988616	4	35	theme	colonic	936:942	arg1	tissue					944:949	colonic tissue	936:949	colonic tissue	936:949	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	2	36	theme	L.	470:471	arg1	BY-05					483:487	L. plantarum BY-05	470:487	L. plantarum BY-05	470:487	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	5	37	theme	microbial	1515:1523	arg1	composition					1525:1535	intestinal microbial composition	1504:1535	intestinal microbial composition	1504:1535	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	4	38	theme	fatty	1210:1214	arg1	acids					1216:1220	short-chain fatty acids	1198:1220	short-chain fatty acids	1198:1220	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	5	39	theme	CONCLUSION	1361:1370	arg1	treatment					1384:1392	CONCLUSION Combination treatment	1361:1392	CONCLUSION Combination treatment with two Lactobacillus strains	1361:1423	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	0	40	dep	Lactobacillus	0:12	arg1	rhamnosus					14:22	rhamnosus	14:22	rhamnosus	14:22	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	4	41	dep	increased	1177:1185	arg1	iii					1172:1174	iii	1172:1174	iii	1172:1174	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	42	theme	several	817:823	arg1	effects					847:853	several additional beneficial effects	817:853	several additional beneficial effects	817:853	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	43	theme	Clostridia	1071:1080	arg1	abundances					1028:1037	abundances	1028:1037	abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae	1028:1118	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	1	44	theme	SCOPE	193:197	arg1	Changes					199:205	SCOPE Changes	193:205	SCOPE Changes in composition of intestinal microbes	193:243	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	4	45	theme	Oscillospiraceae	1083:1098	arg1	abundances					1028:1037	abundances	1028:1037	abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae	1028:1118	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	46	theme	lipopolysaccharides	1264:1282	arg1	content					1236:1242	content	1236:1242	content of pro-inflammatory lipopolysaccharides	1236:1282	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	47	theme	signaling	1342:1350	arg1	pathway					1352:1358	TLR4/NF-κB inflammatory signaling pathway	1318:1358	TLR4/NF-κB inflammatory signaling pathway	1318:1358	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	0	48	theme	microbial	132:140	arg1	composition					142:152	intestinal microbial composition	121:152	intestinal microbial composition	121:152	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	4	49	theme	Akkermansia	1058:1068	arg1	abundances					1028:1037	abundances	1028:1037	abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae	1028:1118	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	3	50	theme	LS	699:700	arg1	treatment					702:710	LS treatment	699:710	LS treatment	699:710	LS treatment suppressed weight loss and colon shortening, and reduced disease activity index in the mice.
36988616	2	51	attach	isolated	519:526	arg2	strains					441:447	combination two Lactobacillus strains	411:447	combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment")	411:505	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	51	attach	isolated	519:526	arg2	"					504:504	"LS treatment"	491:504	"LS treatment"	491:504	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	51	attach	isolated	519:526	arg1	feces					533:537	feces	533:537	feces of healthy human infants	533:562	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	51	attach	isolated	519:526	arg2	BY-02					463:467	L. rhamnosus BY-02	450:467	L. rhamnosus BY-02	450:467	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	1	52	theme	microbes	236:243	arg1	composition					210:220	composition	210:220	composition of intestinal microbes	210:243	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	3	53	theme	colon	739:743	arg1	shortening					745:754	colon shortening	739:754	colon shortening	739:754	LS treatment suppressed weight loss and colon shortening, and reduced disease activity index in the mice.
36988616	0	54	theme	inflammatory	170:181	arg1	response					183:190	inflammatory response	170:190	inflammatory response	170:190	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	4	55	theme	beneficial	836:845	arg1	effects					847:853	several additional beneficial effects	817:853	several additional beneficial effects	817:853	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	56	theme	Escherichia-Shigella	1149:1168	arg1	abundance					1136:1144	abundance	1136:1144	abundance of Escherichia-Shigella	1136:1168	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	57	theme	TLR4/NF-κB	1318:1327	arg1	pathway					1352:1358	TLR4/NF-κB inflammatory signaling pathway	1318:1358	TLR4/NF-κB inflammatory signaling pathway	1318:1358	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	58	theme	Lachnospiraceae	1104:1118	arg1	abundances					1028:1037	abundances	1028:1037	abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae	1028:1118	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	59	theme	intestinal	907:916	arg1	damage					926:931	intestinal barrier damage	907:931	intestinal barrier damage in colonic tissue	907:949	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	0	60	theme	mouse	97:101	arg1	model					103:107	a mouse model	95:107	a mouse model	95:107	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	1	61	theme	disease	362:368	arg1	development					328:338	development	328:338	development of inflammatory bowel disease (IBD)	328:374	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	1	62	from	Changes	199:205	arg1	composition					210:220	composition	210:220	composition of intestinal microbes	210:243	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	4	63	contain	had	813:815	arg1	It					805:806	It	805:806	It	805:806	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	63	contain	had	813:815	arg2	effects					847:853	several additional beneficial effects	817:853	several additional beneficial effects	817:853	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	64	theme	cell	878:881	arg1	numbers					883:889	goblet cell numbers	871:889	goblet cell numbers	871:889	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	65	theme	short-chain	1198:1208	arg1	acids					1216:1220	short-chain fatty acids	1198:1220	short-chain fatty acids	1198:1220	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	3	66	theme	activity	777:784	arg1	index					786:790	disease activity index	769:790	disease activity index in the mice	769:802	LS treatment suppressed weight loss and colon shortening, and reduced disease activity index in the mice.
36988616	2	67	theme	treatment	495:503	arg1	"					504:504	"LS treatment"	491:504	"LS treatment"	491:504	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	67	theme	treatment	495:503	arg1	strains					441:447	combination two Lactobacillus strains	411:447	combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment")	411:505	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	68	dep	METHODS	377:383	arg1	applied					400:406	applied	400:406	applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis	400:628	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	68	dep	METHODS	377:383	arg1	evaluated					635:643	evaluated	635:643	evaluated their ameliorative effect and its possible mechanism	635:696	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	69	dep	BY-02	463:467	arg1	BY-05					483:487	L. plantarum BY-05	470:487	L. plantarum BY-05	470:487	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	70	theme	rhamnosus	453:461	arg1	BY-02					463:467	L. rhamnosus BY-02	450:467	L. rhamnosus BY-02	450:467	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	70	theme	rhamnosus	453:461	arg1	strains					441:447	combination two Lactobacillus strains	411:447	combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment")	411:505	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	71	theme	colitis	622:628	arg1	model					576:580	a mouse model	568:580	a mouse model of dextran sodium sulfate (DSS)-induced colitis	568:628	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	0	72	theme	L.	28:29	arg1	treatment					53:61	Lactobacillus rhamnosus and L. plantarum combination treatment	0:61	Lactobacillus rhamnosus and L. plantarum combination treatment	0:61	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	2	73	theme	Lactobacillus	427:439	arg1	"					504:504	"LS treatment"	491:504	"LS treatment"	491:504	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	73	theme	Lactobacillus	427:439	arg1	BY-02					463:467	L. rhamnosus BY-02	450:467	L. rhamnosus BY-02	450:467	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	73	theme	Lactobacillus	427:439	arg1	strains					441:447	combination two Lactobacillus strains	411:447	combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment")	411:505	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	0	74	theme	combination	41:51	arg1	treatment					53:61	Lactobacillus rhamnosus and L. plantarum combination treatment	0:61	Lactobacillus rhamnosus and L. plantarum combination treatment	0:61	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	5	75	with	treatment	1384:1392	arg1	strains					1417:1423	two Lactobacillus strains	1399:1423	two Lactobacillus strains	1399:1423	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	5	76	theme	inflammatory	1553:1564	arg1	response					1566:1573	inflammatory response	1553:1573	inflammatory response	1553:1573	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	2	77	theme	plantarum	473:481	arg1	BY-05					483:487	L. plantarum BY-05	470:487	L. plantarum BY-05	470:487	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	78	theme	combination	411:421	arg1	"					504:504	"LS treatment"	491:504	"LS treatment"	491:504	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	78	theme	combination	411:421	arg1	BY-02					463:467	L. rhamnosus BY-02	450:467	L. rhamnosus BY-02	450:467	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	2	78	theme	combination	411:421	arg1	strains					441:447	combination two Lactobacillus strains	411:447	combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment")	411:505	METHODS AND RESULTS We applied in combination two Lactobacillus strains (L. rhamnosus BY-02, L. plantarum BY-05) ("LS treatment"), previously isolated from feces of healthy human infants, in a mouse model of dextran sodium sulfate (DSS)-induced colitis, and evaluated their ameliorative effect and its possible mechanism.
36988616	4	79	dep	had	813:815	arg1	v					1286:1286	v	1286:1286	v	1286:1286	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	4	79	dep	had	813:815	arg1	suppressed					1289:1298	suppressed	1289:1298	suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway	1289:1358	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	5	80	theme	intestinal	1504:1513	arg1	composition					1525:1535	intestinal microbial composition	1504:1535	intestinal microbial composition	1504:1535	CONCLUSION Combination treatment with two Lactobacillus strains strongly ameliorated colitis symptoms in our mouse model by favorably altering intestinal microbial composition and suppressing inflammatory response.
36988616	4	81	theme	acids	1216:1220	arg1	content					1187:1193	content	1187:1193	content of short-chain fatty acids	1187:1220	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
36988616	1	82	theme	inflammatory	343:354	arg1	IBD					371:373	IBD	371:373	IBD	371:373	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	1	82	theme	inflammatory	343:354	arg1	disease					362:368	inflammatory bowel disease	343:368	inflammatory bowel disease (IBD)	343:374	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	0	83	dep	L.	28:29	arg1	plantarum					31:39	plantarum	31:39	plantarum	31:39	Lactobacillus rhamnosus and L. plantarum combination treatment ameliorated colitis symptoms in a mouse model by altering intestinal microbial composition and suppressing inflammatory response.
36988616	1	84	with	interaction	278:288	arg1	host					309:312	a susceptible host	295:312	a susceptible host	295:312	SCOPE Changes in composition of intestinal microbes may disrupt the balance of their interaction with a susceptible host, resulting in development of inflammatory bowel disease (IBD).
36988616	4	85	from	damage	926:931	arg1	tissue					944:949	colonic tissue	936:949	colonic tissue	936:949	It also had several additional beneficial effects: (i) maintained goblet cell numbers and ameliorated intestinal barrier damage in colonic tissue; (ii) altered intestinal microbial composition close to normal by increasing abundances of Muribaculaceae, Akkermansia, Clostridia, Oscillospiraceae and Lachnospiraceae, and decreasing abundance of Escherichia-Shigella; (iii) increased content of short-chain fatty acids; (iv) reduced content of pro-inflammatory lipopolysaccharides; (v) suppressed overactivation of TLR4/NF-κB inflammatory signaling pathway.
35358610	3	0	theme	liposomal	451:459	arg1	DPPC					490:493	DPPC	490:493	DPPC	490:493	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	0	theme	liposomal	451:459	arg1	composition					467:477	The liposomal lipid composition	447:477	The liposomal lipid composition (primarily DPPC)	447:494	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	1	theme	cysteine	639:646	arg1	composition					539:549	the lung surfactant composition	519:549	the lung surfactant composition	519:549	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	1	theme	cysteine	639:646	arg1	process					573:579	the drug loading process	556:579	the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively	556:737	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	11	2	theme	acute	2371:2375	arg1	disorders					2346:2354	lung disorders	2341:2354	lung disorders	2341:2354	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	11	2	theme	acute	2371:2375	arg1	ARDS					2408:2411	ARDS	2408:2411	ARDS	2408:2411	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	11	2	theme	acute	2371:2375	arg1	syndrome					2398:2405	acute respiratory distress syndrome	2371:2405	acute respiratory distress syndrome (ARDS)	2371:2412	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	5	3	from	efficacy	1052:1059	arg1	mice					1089:1092	C57BL/6 mice	1081:1092	C57BL/6 mice	1081:1092	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	6	4	theme	ET	1396:1397	arg1	liposomes					1399:1407	ET liposomes	1396:1407	ET liposomes	1396:1407	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	6	5	located	present	1414:1420	arg1	parenchyma					1431:1440	lungs parenchyma	1425:1440	lungs parenchyma	1425:1440	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	6	5	located	present	1414:1420	arg2	liposomes					1399:1407	ET liposomes	1396:1407	ET liposomes	1396:1407	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	4	6	theme	RAW	795:797	arg1	macrophages					805:815	lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages	759:815	lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages	759:815	In vitro, treating lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages with the liposomes decreased TNFα and nitric oxide (NO) secretion, while NAC increased the penetration of nanoparticles through the mucus.
35358610	5	7	dep	intravenous	1105:1115	arg1	administrations					1169:1183	administrations	1169:1183	administrations	1169:1183	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	1	8	theme	alveolar	182:189	arg1	collapse					191:198	alveolar collapse	182:198	alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%	182:285	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	8	9	from	secretion	1810:1818	arg1	lavage					1839:1844	bronchoalveolar lavage	1823:1844	bronchoalveolar lavage (BAL)	1823:1850	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	9	from	secretion	1810:1818	arg1	BAL					1847:1849	BAL	1847:1849	BAL	1847:1849	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	9	10	theme	lipid	2006:2010	arg1	component					2012:2020	the DPPC lipid component	1997:2020	the DPPC lipid component	1997:2020	Interestingly, also the DPPC lipid component alone played a therapeutic role in reducing inflammatory markers in the lungs.
35358610	14	11	theme	delivery	2769:2776	arg1	approaches					2778:2787	both delivery approaches	2764:2787	both delivery approaches	2764:2787	Combining both delivery approaches simultaneously provided the best ARDS treatment outcome.
35358610	13	12	theme	endotracheal	2707:2718	arg1	delivery					2720:2727	endotracheal delivery	2707:2727	endotracheal delivery	2707:2727	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	12	13	theme	mucus	2516:2520	arg1	secretion					2522:2530	mucus secretion	2516:2530	mucus secretion	2516:2530	By co-loading two medications into lipid nanoparticles, we were able to reduce both inflammation and mucus secretion in the inflamed lungs.
35358610	6	14	theme	airflow	1527:1533	arg1	regimes					1535:1541	imperfect airflow regimes	1517:1541	imperfect airflow regimes	1517:1541	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	3	15	theme	mucolytic	657:665	arg1	agent					667:671	a mucolytic agent	655:671	a mucolytic agent	655:671	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	15	theme	mucolytic	657:665	arg1	cysteine					639:646	N-acetyl cysteine	630:646	N-acetyl cysteine (NAC)	630:652	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	16	theme	lung	523:526	arg1	composition					539:549	the lung surfactant composition	519:549	the lung surfactant composition	519:549	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	1	17	theme	Covid-19	103:110	arg1	infections					112:121	Covid-19 infections	103:121	Covid-19 infections	103:121	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	6	18	from	profile	1267:1273	arg1	lungs					1291:1295	the inflamed lungs	1278:1295	the inflamed lungs	1278:1295	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	3	19	theme	drug	560:563	arg1	process					573:579	the drug loading process	556:579	the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively	556:737	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	20	theme	%	723:723	arg1	efficiency					699:708	an encapsulation efficiency	682:708	an encapsulation efficiency of 98% and 92%, respectively	682:737	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	8	21	theme	IL-1β	1795:1799	arg1	secretion					1810:1818	IL-1β cytokine secretion	1795:1818	IL-1β cytokine secretion	1795:1818	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	6	22	from	present	1414:1420	arg1	parenchyma					1431:1440	lungs parenchyma	1425:1440	lungs parenchyma	1425:1440	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	2	23	theme	lung	432:435	arg1	diseases					437:444	lung diseases	432:444	lung diseases	432:444	Here, we describe the development and assessment of 100-nm liposomes that are tailored for pulmonary delivery for treating ARDS, as a model for lung diseases.
35358610	1	24	theme	mortality	261:269	arg1	rates					271:275	patient' mortality rates	252:275	patient' mortality rates	252:275	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	5	25	theme	lung	985:988	arg1	inflammation					990:1001	LPS-induced lung inflammation	973:1001	LPS-induced lung inflammation model	973:1007	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	1	26	theme	diffuse	144:150	arg1	damage					157:162	diffuse lung damage	144:162	diffuse lung damage	144:162	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	11	27	theme	nanoparticles	2314:2326	arg1	delivery					2302:2309	intravenous versus intratracheal delivery	2269:2309	intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS)	2269:2412	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	2	28	dep	development	310:320	arg1	the					306:308	the	306:308	the	306:308	Here, we describe the development and assessment of 100-nm liposomes that are tailored for pulmonary delivery for treating ARDS, as a model for lung diseases.
35358610	8	29	theme	bronchoalveolar	1823:1837	arg1	lavage					1839:1844	bronchoalveolar lavage	1823:1844	bronchoalveolar lavage (BAL)	1823:1850	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	29	theme	bronchoalveolar	1823:1837	arg1	BAL					1847:1849	BAL	1847:1849	BAL	1847:1849	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	13	30	theme	administered	2649:2660	arg1	nanoparticles					2662:2674	intravenously administered nanoparticles	2635:2674	intravenously administered nanoparticles	2635:2674	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	1	31	theme	Distress	61:68	arg1	Syndrome					70:77	Acute Respiratory Distress Syndrome	43:77	Acute Respiratory Distress Syndrome (ARDS)	43:84	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	1	31	theme	Distress	61:68	arg1	ARDS					80:83	ARDS	80:83	ARDS	80:83	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	12	32	theme	lipid	2450:2454	arg1	nanoparticles					2456:2468	lipid nanoparticles	2450:2468	lipid nanoparticles	2450:2468	By co-loading two medications into lipid nanoparticles, we were able to reduce both inflammation and mucus secretion in the inflamed lungs.
35358610	5	33	theme	ET	1152:1153	arg1	nanoparticles					1155:1167	ET nanoparticles	1152:1167	ET nanoparticles	1152:1167	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	2	34	theme	100-nm	340:345	arg1	liposomes					347:355	100-nm liposomes	340:355	100-nm liposomes that are tailored for pulmonary delivery for treating ARDS	340:414	Here, we describe the development and assessment of 100-nm liposomes that are tailored for pulmonary delivery for treating ARDS, as a model for lung diseases.
35358610	6	35	theme	inflamed	1282:1289	arg1	lungs					1291:1295	the inflamed lungs	1278:1295	the inflamed lungs	1278:1295	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	8	36	theme	therapeutic	1694:1704	arg1	effect					1706:1711	a superior therapeutic effect	1683:1711	a superior therapeutic effect	1683:1711	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	6	37	attach	present	1414:1420	arg1	parenchyma					1431:1440	lungs parenchyma	1425:1440	lungs parenchyma	1425:1440	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	6	37	attach	present	1414:1420	arg2	liposomes					1399:1407	ET liposomes	1396:1407	ET liposomes	1396:1407	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	2	38	theme	pulmonary	379:387	arg1	delivery					389:396	pulmonary delivery	379:396	pulmonary delivery for treating ARDS	379:414	Here, we describe the development and assessment of 100-nm liposomes that are tailored for pulmonary delivery for treating ARDS, as a model for lung diseases.
35358610	13	39	theme	lung	2684:2687	arg1	endothelial					2689:2699	lung endothelial	2684:2699	lung endothelial	2684:2699	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	5	40	theme	therapeutic	1040:1050	arg1	efficacy					1052:1059	therapeutic efficacy	1040:1059	therapeutic efficacy	1040:1059	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	6	41	theme	accumulation	1254:1265	arg1	profile					1267:1273	an increased accumulation profile	1241:1273	an increased accumulation profile in the inflamed lungs	1241:1295	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	14	42	theme	ARDS	2822:2825	arg1	outcome					2837:2843	the best ARDS treatment outcome	2813:2843	the best ARDS treatment outcome	2813:2843	Combining both delivery approaches simultaneously provided the best ARDS treatment outcome.
35358610	5	43	theme	C57BL/6	1081:1087	arg1	mice					1089:1092	C57BL/6 mice	1081:1092	C57BL/6 mice	1081:1092	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	8	44	from	inflammation	1763:1774	arg1	lavage					1839:1844	bronchoalveolar lavage	1823:1844	bronchoalveolar lavage (BAL)	1823:1850	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	44	from	inflammation	1763:1774	arg1	BAL					1847:1849	BAL	1847:1849	BAL	1847:1849	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	3	45	theme	lipid	461:465	arg1	DPPC					490:493	DPPC	490:493	DPPC	490:493	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	45	theme	lipid	461:465	arg1	composition					467:477	The liposomal lipid composition	447:477	The liposomal lipid composition (primarily DPPC)	447:494	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	13	46	theme	nanoparticle	2595:2606	arg1	accumulation					2608:2619	high nanoparticle accumulation	2590:2619	high nanoparticle accumulation in the lungs	2590:2632	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	8	47	contain	had	1679:1681	arg2	effect					1706:1711	a superior therapeutic effect	1683:1711	a superior therapeutic effect	1683:1711	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	47	contain	had	1679:1681	arg1	treatment					1669:1677	the nanoparticle treatment	1652:1677	the nanoparticle treatment	1652:1677	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	1	48	theme	Acute	43:47	arg1	Syndrome					70:77	Acute Respiratory Distress Syndrome	43:77	Acute Respiratory Distress Syndrome (ARDS)	43:84	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	1	48	theme	Acute	43:47	arg1	ARDS					80:83	ARDS	80:83	ARDS	80:83	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	4	49	theme	oxide	862:866	arg1	secretion					873:881	nitric oxide (NO) secretion	855:881	nitric oxide (NO) secretion	855:881	In vitro, treating lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages with the liposomes decreased TNFα and nitric oxide (NO) secretion, while NAC increased the penetration of nanoparticles through the mucus.
35358610	14	50	theme	best	2817:2820	arg1	outcome					2837:2843	the best ARDS treatment outcome	2813:2843	the best ARDS treatment outcome	2813:2843	Combining both delivery approaches simultaneously provided the best ARDS treatment outcome.
35358610	5	51	dep	endotracheal	1123:1134	arg1	ET					1137:1138	ET	1137:1138	ET	1137:1138	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	4	52	theme	nanoparticles	923:935	arg1	penetration					908:918	the penetration	904:918	the penetration of nanoparticles through the mucus	904:953	In vitro, treating lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages with the liposomes decreased TNFα and nitric oxide (NO) secretion, while NAC increased the penetration of nanoparticles through the mucus.
35358610	8	53	from	IL-6	1786:1789	arg1	lavage					1839:1844	bronchoalveolar lavage	1823:1844	bronchoalveolar lavage (BAL)	1823:1850	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	53	from	IL-6	1786:1789	arg1	BAL					1847:1849	BAL	1847:1849	BAL	1847:1849	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	54	theme	free	1725:1728	arg1	drugs					1730:1734	free drugs	1725:1734	free drugs	1725:1734	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	13	55	theme	delivery	2569:2576	arg1	modes					2560:2564	Both modes	2555:2564	Both modes of delivery	2555:2576	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	5	56	theme	liposomes	1068:1076	arg1	accumulation					1023:1034	accumulation	1023:1034	accumulation	1023:1034	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	5	56	theme	liposomes	1068:1076	arg1	efficacy					1052:1059	therapeutic efficacy	1040:1059	therapeutic efficacy	1040:1059	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	7	57	theme	inflammation	1624:1635	arg1	markers					1613:1619	markers	1613:1619	markers of inflammation	1613:1635	Twenty hours after the different treatments, lungs were assessed for markers of inflammation.
35358610	8	58	from	TNFα	1780:1783	arg1	lavage					1839:1844	bronchoalveolar lavage	1823:1844	bronchoalveolar lavage (BAL)	1823:1850	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	58	from	TNFα	1780:1783	arg1	BAL					1847:1849	BAL	1847:1849	BAL	1847:1849	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	14	59	theme	treatment	2827:2835	arg1	outcome					2837:2843	the best ARDS treatment outcome	2813:2843	the best ARDS treatment outcome	2813:2843	Combining both delivery approaches simultaneously provided the best ARDS treatment outcome.
35358610	1	60	theme	lung	152:155	arg1	damage					157:162	diffuse lung damage	144:162	diffuse lung damage	144:162	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	3	61	theme	N-acetyl	630:637	arg1	agent					667:671	a mucolytic agent	655:671	a mucolytic agent	655:671	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	61	theme	N-acetyl	630:637	arg1	NAC					649:651	NAC	649:651	NAC	649:651	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	61	theme	N-acetyl	630:637	arg1	cysteine					639:646	N-acetyl cysteine	630:646	N-acetyl cysteine (NAC)	630:652	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	5	62	from	accumulation	1023:1034	arg1	mice					1089:1092	C57BL/6 mice	1081:1092	C57BL/6 mice	1081:1092	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	9	63	from	markers	2079:2085	arg1	lungs					2094:2098	the lungs	2090:2098	the lungs	2090:2098	Interestingly, also the DPPC lipid component alone played a therapeutic role in reducing inflammatory markers in the lungs.
35358610	4	64	theme	-stimulated	783:793	arg1	macrophages					805:815	lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages	759:815	lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages	759:815	In vitro, treating lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages with the liposomes decreased TNFα and nitric oxide (NO) secretion, while NAC increased the penetration of nanoparticles through the mucus.
35358610	11	65	theme	respiratory	2377:2387	arg1	disorders					2346:2354	lung disorders	2341:2354	lung disorders	2341:2354	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	11	65	theme	respiratory	2377:2387	arg1	ARDS					2408:2411	ARDS	2408:2411	ARDS	2408:2411	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	11	65	theme	respiratory	2377:2387	arg1	syndrome					2398:2405	acute respiratory distress syndrome	2371:2405	acute respiratory distress syndrome (ARDS)	2371:2412	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	9	66	theme	DPPC	2001:2004	arg1	component					2012:2020	the DPPC lipid component	1997:2020	the DPPC lipid component	1997:2020	Interestingly, also the DPPC lipid component alone played a therapeutic role in reducing inflammatory markers in the lungs.
35358610	3	67	theme	surfactant	528:537	arg1	composition					539:549	the lung surfactant composition	519:549	the lung surfactant composition	519:549	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	6	68	located	detected	1455:1462	arg1	regions					1479:1485	some distal regions	1467:1485	some distal regions of the lungs	1467:1498	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	6	68	located	detected	1455:1462	arg2	liposomes					1399:1407	ET liposomes	1396:1407	ET liposomes	1396:1407	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	10	69	theme	therapeutic	2128:2138	arg1	nanoparticles					2140:2152	therapeutic nanoparticles	2128:2152	therapeutic nanoparticles	2128:2152	Collectively, we show that therapeutic nanoparticles accumulate in inflamed lungs holding potential for treating lung disorders.
35358610	12	70	from	inflammation	2499:2510	arg1	lungs					2548:2552	the inflamed lungs	2535:2552	the inflamed lungs	2535:2552	By co-loading two medications into lipid nanoparticles, we were able to reduce both inflammation and mucus secretion in the inflamed lungs.
35358610	12	71	theme	inflamed	2539:2546	arg1	lungs					2548:2552	the inflamed lungs	2535:2552	the inflamed lungs	2535:2552	By co-loading two medications into lipid nanoparticles, we were able to reduce both inflammation and mucus secretion in the inflamed lungs.
35358610	6	72	theme	imperfect	1517:1525	arg1	regimes					1535:1541	imperfect airflow regimes	1517:1541	imperfect airflow regimes	1517:1541	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	13	73	theme	lung	2737:2740	arg1	epithelial					2742:2751	lung epithelial	2737:2751	lung epithelial	2737:2751	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	3	74	dep	DPPC	490:493	arg1	primarily					480:488	primarily	480:488	primarily	480:488	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	10	75	theme	inflamed	2168:2175	arg1	lungs					2177:2181	inflamed lungs	2168:2181	inflamed lungs holding potential for treating lung disorders	2168:2227	Collectively, we show that therapeutic nanoparticles accumulate in inflamed lungs holding potential for treating lung disorders.
35358610	11	76	theme	intratracheal	2288:2300	arg1	delivery					2302:2309	intravenous versus intratracheal delivery	2269:2309	intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS)	2269:2412	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	9	77	theme	therapeutic	2037:2047	arg1	role					2049:2052	a therapeutic role	2035:2052	a therapeutic role	2035:2052	Interestingly, also the DPPC lipid component alone played a therapeutic role in reducing inflammatory markers in the lungs.
35358610	8	78	theme	cytokine	1801:1808	arg1	secretion					1810:1818	IL-1β cytokine secretion	1795:1818	IL-1β cytokine secretion	1795:1818	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	6	79	theme	lungs	1425:1429	arg1	parenchyma					1431:1440	lungs parenchyma	1425:1440	lungs parenchyma	1425:1440	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	10	80	theme	lung	2214:2217	arg1	disorders					2219:2227	lung disorders	2214:2227	lung disorders	2214:2227	Collectively, we show that therapeutic nanoparticles accumulate in inflamed lungs holding potential for treating lung disorders.
35358610	11	81	theme	intravenous	2269:2279	arg1	delivery					2302:2309	intravenous versus intratracheal delivery	2269:2309	intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS)	2269:2412	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	3	82	theme	encapsulation	685:697	arg1	efficiency					699:708	an encapsulation efficiency	682:708	an encapsulation efficiency of 98% and 92%, respectively	682:737	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	5	83	theme	LPS-induced	973:983	arg1	inflammation					990:1001	LPS-induced lung inflammation	973:1001	LPS-induced lung inflammation model	973:1007	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	6	84	from	parenchyma	1431:1440	arg1	present					1414:1420	present	1414:1420	present	1414:1420	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	7	85	theme	different	1567:1575	arg1	treatments					1577:1586	the different treatments	1563:1586	the different treatments	1563:1586	Twenty hours after the different treatments, lungs were assessed for markers of inflammation.
35358610	4	86	theme	nitric	855:860	arg1	NO					869:870	NO	869:870	NO	869:870	In vitro, treating lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages with the liposomes decreased TNFα and nitric oxide (NO) secretion, while NAC increased the penetration of nanoparticles through the mucus.
35358610	4	86	theme	nitric	855:860	arg1	oxide					862:866	nitric oxide	855:866	nitric oxide (NO) secretion	855:881	In vitro, treating lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages with the liposomes decreased TNFα and nitric oxide (NO) secretion, while NAC increased the penetration of nanoparticles through the mucus.
35358610	5	87	dep	accumulation	1023:1034	arg1	the					1019:1021	the	1019:1021	the	1019:1021	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	8	88	contain	had	1937:1939	arg1	treatment					1875:1883	the combined treatment	1862:1883	the combined treatment	1862:1883	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	88	contain	had	1937:1939	arg2	outcome					1950:1956	the best outcome	1941:1956	the best outcome of all treatments	1941:1974	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	9	89	theme	inflammatory	2066:2077	arg1	markers					2079:2085	inflammatory markers	2066:2085	inflammatory markers in the lungs	2066:2098	Interestingly, also the DPPC lipid component alone played a therapeutic role in reducing inflammatory markers in the lungs.
35358610	6	90	theme	administration	1197:1210	arg1	methods					1212:1218	both administration methods	1192:1218	both administration methods	1192:1218	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	5	91	theme	inflammation	990:1001	arg1	model					1003:1007	LPS-induced lung inflammation model	973:1007	LPS-induced lung inflammation model	973:1007	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	6	92	theme	lungs	1494:1498	arg1	regions					1479:1485	some distal regions	1467:1485	some distal regions of the lungs	1467:1498	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	1	93	theme	Respiratory	49:59	arg1	Syndrome					70:77	Acute Respiratory Distress Syndrome	43:77	Acute Respiratory Distress Syndrome (ARDS)	43:84	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	1	93	theme	Respiratory	49:59	arg1	ARDS					80:83	ARDS	80:83	ARDS	80:83	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	3	94	theme	%	715:715	arg1	efficiency					699:708	an encapsulation efficiency	682:708	an encapsulation efficiency of 98% and 92%, respectively	682:737	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	8	95	theme	nanoparticle	1656:1667	arg1	treatment					1669:1677	the nanoparticle treatment	1652:1677	the nanoparticle treatment	1652:1677	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	13	96	theme	high	2590:2593	arg1	accumulation					2608:2619	high nanoparticle accumulation	2590:2619	high nanoparticle accumulation in the lungs	2590:2632	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	5	97	used	used	968:971	arg2	we					965:966	we	965:966	we	965:966	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	11	98	theme	lung	2341:2344	arg1	disorders					2346:2354	lung disorders	2341:2354	lung disorders	2341:2354	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	11	98	theme	lung	2341:2344	arg1	syndrome					2398:2405	acute respiratory distress syndrome	2371:2405	acute respiratory distress syndrome (ARDS)	2371:2412	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	2	99	theme	liposomes	347:355	arg1	development					310:320	development	310:320	development	310:320	Here, we describe the development and assessment of 100-nm liposomes that are tailored for pulmonary delivery for treating ARDS, as a model for lung diseases.
35358610	2	99	theme	liposomes	347:355	arg1	model					422:426	a model	420:426	a model for lung diseases	420:444	Here, we describe the development and assessment of 100-nm liposomes that are tailored for pulmonary delivery for treating ARDS, as a model for lung diseases.
35358610	2	99	theme	liposomes	347:355	arg1	assessment					326:335	assessment	326:335	assessment	326:335	Here, we describe the development and assessment of 100-nm liposomes that are tailored for pulmonary delivery for treating ARDS, as a model for lung diseases.
35358610	6	100	theme	distal	1472:1477	arg1	regions					1479:1485	some distal regions	1467:1485	some distal regions of the lungs	1467:1498	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	11	101	theme	distress	2389:2396	arg1	disorders					2346:2354	lung disorders	2341:2354	lung disorders	2341:2354	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	11	101	theme	distress	2389:2396	arg1	ARDS					2408:2411	ARDS	2408:2411	ARDS	2408:2411	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	11	101	theme	distress	2389:2396	arg1	syndrome					2398:2405	acute respiratory distress syndrome	2371:2405	acute respiratory distress syndrome (ARDS)	2371:2412	SIGNIFICANCE: In this study we compare intravenous versus intratracheal delivery of nanoparticles for treating lung disorders, specifically, acute respiratory distress syndrome (ARDS).
35358610	5	102	dep	mice	1089:1092	arg1	either					1095:1100	either	1095:1100	either	1095:1100	In vivo, we used LPS-induced lung inflammation model to assess the accumulation and therapeutic efficacy of the liposomes in C57BL/6 mice, either by intravenous (IV), endotracheal (ET) or IV plus ET nanoparticles administrations.
35358610	8	103	theme	superior	1685:1692	arg1	effect					1706:1711	a superior therapeutic effect	1683:1711	a superior therapeutic effect	1683:1711	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	8	104	theme	combined	1866:1873	arg1	treatment					1875:1883	the combined treatment	1862:1883	the combined treatment	1862:1883	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	3	105	theme	loading	565:571	arg1	process					573:579	the drug loading process	556:579	the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively	556:737	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	6	106	theme	increased	1244:1252	arg1	profile					1267:1273	an increased accumulation profile	1241:1273	an increased accumulation profile in the inflamed lungs	1241:1295	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	12	107	from	secretion	2522:2530	arg1	lungs					2548:2552	the inflamed lungs	2535:2552	the inflamed lungs	2535:2552	By co-loading two medications into lipid nanoparticles, we were able to reduce both inflammation and mucus secretion in the inflamed lungs.
35358610	8	108	theme	best	1945:1948	arg1	outcome					1950:1956	the best outcome	1941:1956	the best outcome of all treatments	1941:1974	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35358610	13	109	from	accumulation	2608:2619	arg1	lungs					2628:2632	the lungs	2624:2632	the lungs	2624:2632	Both modes of delivery resulted in high nanoparticle accumulation in the lungs, intravenously administered nanoparticles reached lung endothelial while endotracheal delivery reached lung epithelial.
35358610	3	110	theme	methylprednisolone	589:606	arg1	composition					539:549	the lung surfactant composition	519:549	the lung surfactant composition	519:549	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	3	110	theme	methylprednisolone	589:606	arg1	process					573:579	the drug loading process	556:579	the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively	556:737	The liposomal lipid composition (primarily DPPC) was optimized to mimic the lung surfactant composition, and the drug loading process of both methylprednisolone (MPS), a steroid, and N-acetyl cysteine (NAC), a mucolytic agent, reached an encapsulation efficiency of 98% and 92%, respectively.
35358610	6	111	theme	IV-administrated	1329:1344	arg1	liposomes					1346:1354	IV-administrated liposomes	1329:1354	IV-administrated liposomes distributed widely throughout the lung	1329:1393	Using both administration methods, liposomes exhibited an increased accumulation profile in the inflamed lungs over 48 h. Interestingly, while IV-administrated liposomes distributed widely throughout the lung, ET liposomes were present in lungs parenchyma but were not detected at some distal regions of the lungs, possibly due to imperfect airflow regimes.
35358610	1	112	theme	gas	213:215	arg1	exchange					217:224	gas exchange	213:224	gas exchange	213:224	Acute Respiratory Distress Syndrome (ARDS), associated with Covid-19 infections, is characterized by diffuse lung damage, inflammation and alveolar collapse that impairs gas exchange, leading to hypoxemia and patient' mortality rates above 40%.
35358610	8	113	theme	treatments	1965:1974	arg1	outcome					1950:1956	the best outcome	1941:1956	the best outcome of all treatments	1941:1974	We found that the nanoparticle treatment had a superior therapeutic effect compared to free drugs in treating ARDS, reducing inflammation and TNFα, IL-6 and IL-1β cytokine secretion in bronchoalveolar lavage (BAL), and that the combined treatment, delivering nanoparticles IV and ET simultaneously, had the best outcome of all treatments.
35157708	0	0	theme	joint	83:87	arg1	ligaments					89:97	knee joint ligaments	78:97	knee joint ligaments	78:97	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	4	1	theme	articular	704:712	arg1	cartilage					714:722	articular cartilage	704:722	articular cartilage	704:722	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	9	2	theme	ligaments	1380:1388	arg1	response					1368:1375	the mechanical response	1353:1375	the mechanical response of ligaments	1353:1388	As collagen largely determines the mechanical response of ligaments, we conclude that NIRS demonstrates potential for quantitative evaluation of knee ligaments.
35157708	3	3	theme	arthroscopic	477:488	arg1	evaluation					490:499	the arthroscopic evaluation	473:499	the arthroscopic evaluation	473:499	While the condition of knee ligaments can be examined during arthroscopic repair procedures, the arthroscopic evaluation suffers from subjectivity and poor repeatability.
35157708	6	4	dep	measured	923:930	arg1	followed					943:950	followed	943:950	followed by chemical and histological reference analysis	943:998	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	0	5	theme	knee	78:81	arg1	ligaments					89:97	knee joint ligaments	78:97	knee joint ligaments	78:97	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	1	6	theme	important	155:163	arg1	role					165:168	an important role	152:168	an important role	152:168	Knee ligaments and tendons play an important role in stabilizing and controlling the motions of the knee.
35157708	4	7	dep	composition	633:643	arg1	the					629:631	the	629:631	the	629:631	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	2	8	dep	cartilage	331:339	arg1	e.g.					325:328	e.g.	325:328	e.g.	325:328	Injuries to the ligaments can lead to abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus) and even osteoarthritis.
35157708	0	9	theme	ligaments	89:97	arg1	biochemical					42:52	biochemical	42:52	biochemical	42:52	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	6	10	theme	joints	911:916	arg1	ligaments					860:868	ligaments	860:868	ligaments	860:868	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	6	10	theme	joints	911:916	arg1	tendon					883:888	patellar tendon	874:888	patellar tendon	874:888	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	2	11	theme	abnormal	264:271	arg1	loading					284:290	abnormal mechanical loading	264:290	abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus)	264:353	Injuries to the ligaments can lead to abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus) and even osteoarthritis.
35157708	0	12	theme	tendon	112:117	arg1	biochemical					42:52	biochemical	42:52	biochemical	42:52	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	6	13	theme	stifle	904:909	arg1	joints					911:916	ten bovine stifle joints	893:916	ten bovine stifle joints	893:916	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	7	14	theme	squares	1118:1124	arg1	regression					1126:1135	partial least squares regression	1104:1135	partial least squares regression	1104:1135	The relationship between the reference properties of the tissue and NIR spectra was investigated using partial least squares regression.
35157708	9	15	theme	knee	1467:1470	arg1	ligaments					1472:1480	knee ligaments	1467:1480	knee ligaments	1467:1480	As collagen largely determines the mechanical response of ligaments, we conclude that NIRS demonstrates potential for quantitative evaluation of knee ligaments.
35157708	9	16	theme	quantitative	1440:1451	arg1	evaluation					1453:1462	quantitative evaluation	1440:1462	quantitative evaluation of knee ligaments	1440:1480	As collagen largely determines the mechanical response of ligaments, we conclude that NIRS demonstrates potential for quantitative evaluation of knee ligaments.
35157708	3	17	theme	ligaments	408:416	arg1	condition					390:398	the condition	386:398	the condition of knee ligaments	386:416	While the condition of knee ligaments can be examined during arthroscopic repair procedures, the arthroscopic evaluation suffers from subjectivity and poor repeatability.
35157708	0	18	theme	patellar	103:110	arg1	tendon					112:117	patellar tendon	103:117	patellar tendon	103:117	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	6	19	theme	bovine	897:902	arg1	joints					911:916	ten bovine stifle joints	893:916	ten bovine stifle joints	893:916	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	5	20	theme	composition	798:808	arg1	evaluation					775:784	NIRS-based evaluation	764:784	NIRS-based evaluation of ligament composition	764:808	Despite the similarities, NIRS-based evaluation of ligament composition has not been previously attempted.
35157708	8	21	theme	=	1193:1193	arg1	water					1181:1185	the water (R2CV = .65) and collagen (R2CV = .57) contents	1177:1233	water	1181:1185	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
35157708	8	21	theme	=	1193:1193	arg1	R2CV					1188:1191	R2CV = .65	1188:1197	R2CV = .65	1188:1197	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
35157708	0	22	theme	infrared	5:12	arg1	evaluation					28:37	Near infrared spectroscopic evaluation	0:37	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.	0:118	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	5	23	theme	ligament	789:796	arg1	composition					798:808	ligament composition	789:808	ligament composition	789:808	Despite the similarities, NIRS-based evaluation of ligament composition has not been previously attempted.
35157708	7	24	theme	partial	1104:1110	arg1	regression					1126:1135	partial least squares regression	1104:1135	partial least squares regression	1104:1135	The relationship between the reference properties of the tissue and NIR spectra was investigated using partial least squares regression.
35157708	2	25	theme	other	299:303	arg1	tissues					316:322	the other supporting tissues	295:322	the other supporting tissues (e.g., cartilage and meniscus)	295:353	Injuries to the ligaments can lead to abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus) and even osteoarthritis.
35157708	7	26	theme	NIR	1069:1071	arg1	spectra					1073:1079	the tissue and NIR spectra	1054:1079	the tissue and NIR spectra	1054:1079	The relationship between the reference properties of the tissue and NIR spectra was investigated using partial least squares regression.
35157708	4	27	theme	tissues	687:693	arg1	structure					649:657	structure	649:657	structure	649:657	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	4	27	theme	tissues	687:693	arg1	composition					633:643	composition	633:643	composition	633:643	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	0	28	theme	Near	0:3	arg1	evaluation					28:37	Near infrared spectroscopic evaluation	0:37	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.	0:118	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	4	29	theme	Near	551:554	arg1	NIRS					579:582	NIRS	579:582	NIRS	579:582	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	4	29	theme	Near	551:554	arg1	spectroscopy					565:576	Near infrared spectroscopy	551:576	Near infrared spectroscopy (NIRS)	551:583	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	7	30	theme	least	1112:1116	arg1	regression					1126:1135	partial least squares regression	1104:1135	partial least squares regression	1104:1135	The relationship between the reference properties of the tissue and NIR spectra was investigated using partial least squares regression.
35157708	3	31	theme	knee	403:406	arg1	ligaments					408:416	knee ligaments	403:416	knee ligaments	403:416	While the condition of knee ligaments can be examined during arthroscopic repair procedures, the arthroscopic evaluation suffers from subjectivity and poor repeatability.
35157708	6	32	theme	reference	981:989	arg1	analysis					991:998	chemical and histological reference analysis	955:998	chemical and histological reference analysis	955:998	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	0	33	theme	spectroscopic	14:26	arg1	evaluation					28:37	Near infrared spectroscopic evaluation	0:37	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.	0:118	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	6	34	theme	histological	968:979	arg1	analysis					991:998	chemical and histological reference analysis	955:998	chemical and histological reference analysis	955:998	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	3	35	theme	arthroscopic	441:452	arg1	procedures					461:470	arthroscopic repair procedures	441:470	arthroscopic repair procedures	441:470	While the condition of knee ligaments can be examined during arthroscopic repair procedures, the arthroscopic evaluation suffers from subjectivity and poor repeatability.
35157708	0	36	theme	biochemical	42:52	arg1	evaluation					28:37	Near infrared spectroscopic evaluation	0:37	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.	0:118	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	4	37	theme	infrared	556:563	arg1	NIRS					579:582	NIRS	579:582	NIRS	579:582	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	4	37	theme	infrared	556:563	arg1	spectroscopy					565:576	Near infrared spectroscopy	551:576	Near infrared spectroscopy (NIRS)	551:583	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	7	38	theme	spectra	1073:1079	arg1	properties					1040:1049	the reference properties	1026:1049	the reference properties of the tissue and NIR spectra	1026:1079	The relationship between the reference properties of the tissue and NIR spectra was investigated using partial least squares regression.
35157708	4	39	theme	connective	676:685	arg1	meniscus					728:735	meniscus	728:735	meniscus	728:735	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	4	39	theme	connective	676:685	arg1	cartilage					714:722	articular cartilage	704:722	articular cartilage	704:722	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	4	39	theme	connective	676:685	arg1	tissues					687:693	collagen-rich connective tissues	662:693	collagen-rich connective tissues	662:693	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	9	40	theme	ligaments	1472:1480	arg1	evaluation					1453:1462	quantitative evaluation	1440:1462	quantitative evaluation of knee ligaments	1440:1480	As collagen largely determines the mechanical response of ligaments, we conclude that NIRS demonstrates potential for quantitative evaluation of knee ligaments.
35157708	4	41	theme	collagen-rich	662:674	arg1	meniscus					728:735	meniscus	728:735	meniscus	728:735	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	4	41	theme	collagen-rich	662:674	arg1	cartilage					714:722	articular cartilage	704:722	articular cartilage	704:722	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	4	41	theme	collagen-rich	662:674	arg1	tissues					687:693	collagen-rich connective tissues	662:693	collagen-rich connective tissues	662:693	Near infrared spectroscopy (NIRS) is capable of non-destructively quantifying the composition and structure of collagen-rich connective tissues, such as articular cartilage and meniscus.
35157708	6	42	theme	chemical	955:962	arg1	analysis					991:998	chemical and histological reference analysis	955:998	chemical and histological reference analysis	955:998	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	8	43	theme	collagen	1204:1211	arg1	contents					1226:1233	the water (R2CV = .65) and collagen (R2CV = .57) contents	1177:1233	contents	1226:1233	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
35157708	8	43	theme	collagen	1204:1211	arg1	R2CV					1214:1217	R2CV = .57	1214:1223	R2CV = .57	1214:1223	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
35157708	6	44	theme	patellar	874:881	arg1	tendon					883:888	patellar tendon	874:888	patellar tendon	874:888	In this study, ligaments and patellar tendon of ten bovine stifle joints were measured with NIRS, followed by chemical and histological reference analysis.
35157708	0	45	theme	crimp	58:62	arg1	evaluation					28:37	Near infrared spectroscopic evaluation	0:37	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.	0:118	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	0	46	dep	biochemical	42:52	arg1	properties					64:73	properties	64:73	properties	64:73	Near infrared spectroscopic evaluation of biochemical and crimp properties of knee joint ligaments and patellar tendon.
35157708	9	47	theme	mechanical	1357:1366	arg1	response					1368:1375	the mechanical response	1353:1375	the mechanical response of ligaments	1353:1388	As collagen largely determines the mechanical response of ligaments, we conclude that NIRS demonstrates potential for quantitative evaluation of knee ligaments.
35157708	3	48	theme	repair	454:459	arg1	procedures					461:470	arthroscopic repair procedures	441:470	arthroscopic repair procedures	441:470	While the condition of knee ligaments can be examined during arthroscopic repair procedures, the arthroscopic evaluation suffers from subjectivity and poor repeatability.
35157708	1	49	theme	Knee	120:123	arg1	ligaments					125:133	Knee ligaments	120:133	Knee ligaments	120:133	Knee ligaments and tendons play an important role in stabilizing and controlling the motions of the knee.
35157708	7	50	theme	reference	1030:1038	arg1	properties					1040:1049	the reference properties	1026:1049	the reference properties of the tissue and NIR spectra	1026:1079	The relationship between the reference properties of the tissue and NIR spectra was investigated using partial least squares regression.
35157708	2	51	theme	tissues	316:322	arg1	osteoarthritis					364:377	osteoarthritis	364:377	osteoarthritis	364:377	Injuries to the ligaments can lead to abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus) and even osteoarthritis.
35157708	2	51	theme	tissues	316:322	arg1	loading					284:290	abnormal mechanical loading	264:290	abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus)	264:353	Injuries to the ligaments can lead to abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus) and even osteoarthritis.
35157708	7	52	theme	tissue	1058:1063	arg1	spectra					1073:1079	the tissue and NIR spectra	1054:1079	the tissue and NIR spectra	1054:1079	The relationship between the reference properties of the tissue and NIR spectra was investigated using partial least squares regression.
35157708	2	53	theme	mechanical	273:282	arg1	loading					284:290	abnormal mechanical loading	264:290	abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus)	264:353	Injuries to the ligaments can lead to abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus) and even osteoarthritis.
35157708	2	54	theme	supporting	305:314	arg1	tissues					316:322	the other supporting tissues	295:322	the other supporting tissues (e.g., cartilage and meniscus)	295:353	Injuries to the ligaments can lead to abnormal mechanical loading of the other supporting tissues (e.g., cartilage and meniscus) and even osteoarthritis.
35157708	1	55	theme	knee	220:223	arg1	motions					205:211	the motions	201:211	the motions of the knee	201:223	Knee ligaments and tendons play an important role in stabilizing and controlling the motions of the knee.
35157708	5	56	theme	NIRS-based	764:773	arg1	evaluation					775:784	NIRS-based evaluation	764:784	NIRS-based evaluation of ligament composition	764:808	Despite the similarities, NIRS-based evaluation of ligament composition has not been previously attempted.
35157708	8	57	theme	internal	1274:1281	arg1	structure					1289:1297	the internal crimp structure	1270:1297	the internal crimp structure	1270:1297	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
35157708	8	58	theme	=	1219:1219	arg1	contents					1226:1233	the water (R2CV = .65) and collagen (R2CV = .57) contents	1177:1233	contents	1226:1233	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
35157708	8	58	theme	=	1219:1219	arg1	R2CV					1214:1217	R2CV = .57	1214:1223	R2CV = .57	1214:1223	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
35157708	3	59	theme	poor	531:534	arg1	repeatability					536:548	poor repeatability	531:548	poor repeatability	531:548	While the condition of knee ligaments can be examined during arthroscopic repair procedures, the arthroscopic evaluation suffers from subjectivity and poor repeatability.
35157708	8	60	theme	crimp	1283:1287	arg1	structure					1289:1297	the internal crimp structure	1270:1297	the internal crimp structure	1270:1297	NIRS was found to be sensitive towards the water (R2CV = .65) and collagen (R2CV = .57) contents, while elastin, proteoglycans, and the internal crimp structure remained undetectable.
36476377	1	0	with	patients	275:282	arg1	depression					289:298	depression	289:298	depression	289:298	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	8	1	theme	detailed	1600:1607	arg1	study					1609:1613	further detailed study	1592:1613	further detailed study	1592:1613	It is, therefore, unlikely that 5-HT2AR may play a major role in rapid-acting antidepressant actions of psychedelics although further detailed study is needed.
36476377	3	2	theme	decreased	720:728	arg1	density					746:752	the decreased dendritic spine density	716:752	the decreased dendritic spine density	716:752	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	3	from	density	1121:1127	arg1	region					1146:1151	the prelimbic region	1132:1151	the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1132:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	5	4	theme	medial	1156:1161	arg1	cortex					1174:1179	medial prefrontal cortex	1156:1179	medial prefrontal cortex	1156:1179	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	7	5	theme	psychedelic	1445:1455	arg1	effects					1457:1463	5-HT2AR-related psychedelic effects	1429:1463	5-HT2AR-related psychedelic effects	1429:1463	This study suggests that antidepressant-like effect of lisuride in LPS-treated mice is not associated with 5-HT2AR-related psychedelic effects.
36476377	7	6	theme	LPS-treated	1389:1399	arg1	mice					1401:1404	LPS-treated mice	1389:1404	LPS-treated mice	1389:1404	This study suggests that antidepressant-like effect of lisuride in LPS-treated mice is not associated with 5-HT2AR-related psychedelic effects.
36476377	2	7	theme	ongoing	322:328	arg1	debate					330:335	an ongoing debate	319:335	an ongoing debate over the role of 5-HT2AR in the antidepressant-like actions of psychedelics	319:411	However, there is an ongoing debate over the role of 5-HT2AR in the antidepressant-like actions of psychedelics.
36476377	5	8	theme	cortex	1174:1179	arg1	region					1146:1151	the prelimbic region	1132:1151	the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1132:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	7	9	theme	lisuride	1377:1384	arg1	effect					1367:1372	antidepressant-like effect	1347:1372	antidepressant-like effect of lisuride in LPS-treated mice	1347:1404	This study suggests that antidepressant-like effect of lisuride in LPS-treated mice is not associated with 5-HT2AR-related psychedelic effects.
36476377	3	10	theme	non-hallucinogenic	568:585	arg1	analog					599:604	non-hallucinogenic psychedelic analog	568:604	non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms	568:638	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	1	11	theme	5-HT2AR	221:227	arg1	agonism					230:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	3	12	theme	5-HT1AR	623:629	arg1	agonisms					631:638	5-HT2AR and 5-HT1AR agonisms	611:638	5-HT2AR and 5-HT1AR agonisms	611:638	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	13	theme	lipopolysaccharide	770:787	arg1	mice					803:806	lipopolysaccharide (LPS)-treated mice	770:806	lipopolysaccharide (LPS)-treated mice	770:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	4	14	dep	LPS	932:934	arg1	23 h					948:951	23 h	948:951	23 h before	948:958	Saline (10 ml/kg), DOI (2.0 mg/kg), lisuride (1.0 mg/kg), or (R)-ketamine (10 mg/kg) was administered intraperitoneally to LPS (0.5 mg/kg, 23 h before)-treated mice.
36476377	4	14	dep	LPS	932:934	arg1	0.5 mg/kg					937:945	0.5 mg/kg	937:945	0.5 mg/kg	937:945	Saline (10 ml/kg), DOI (2.0 mg/kg), lisuride (1.0 mg/kg), or (R)-ketamine (10 mg/kg) was administered intraperitoneally to LPS (0.5 mg/kg, 23 h before)-treated mice.
36476377	3	15	theme	5-HT2AR	611:617	arg1	agonisms					631:638	5-HT2AR and 5-HT1AR agonisms	611:638	5-HT2AR and 5-HT1AR agonisms	611:638	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	1	16	theme	rapid	243:247	arg1	actions					264:270	rapid antidepressant actions	243:270	rapid antidepressant actions	243:270	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	5	17	theme	increased	1036:1044	arg1	time					1057:1060	the increased immobility time	1032:1060	the increased immobility time of forced swimming test	1032:1084	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	18	from	density	746:752	arg1	brain					761:765	the brain	757:765	the brain of lipopolysaccharide (LPS)-treated mice	757:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	19	theme	-treated	794:801	arg1	mice					803:806	lipopolysaccharide (LPS)-treated mice	770:806	lipopolysaccharide (LPS)-treated mice	770:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	20	theme	mice	1234:1237	arg1	hippocampus					1207:1217	hippocampus	1207:1217	hippocampus of LPS-treated mice	1207:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	1	21	theme	Classical	160:168	arg1	psychedelics					170:181	Classical psychedelics	160:181	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	160:236	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	7	22	from	effect	1367:1372	arg1	mice					1401:1404	LPS-treated mice	1389:1404	LPS-treated mice	1389:1404	This study suggests that antidepressant-like effect of lisuride in LPS-treated mice is not associated with 5-HT2AR-related psychedelic effects.
36476377	5	23	theme	test	1081:1084	arg1	time					1057:1060	the increased immobility time	1032:1060	the increased immobility time of forced swimming test	1032:1084	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	5	23	theme	test	1081:1084	arg1	density					1121:1127	the decreased dendritic spine density	1091:1127	the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1091:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	24	theme	novel	650:654	arg1	-ketamine					674:682	the novel antidepressant (R)-ketamine	646:682	the novel antidepressant (R)-ketamine	646:682	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	0	25	from	effect	26:31	arg1	mice					154:157	lipopolysaccharide-treated mice	127:157	lipopolysaccharide-treated mice	127:157	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	1	26	theme	receptor	211:218	arg1	agonism					230:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	0	27	theme	psychedelic	107:117	arg1	DOI					119:121	hallucinogenic psychedelic DOI	92:121	hallucinogenic psychedelic DOI	92:121	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	5	28	theme	forced	1065:1070	arg1	test					1081:1084	forced swimming test	1065:1084	forced swimming test	1065:1084	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	0	29	theme	Rapid	0:4	arg1	effect					26:31	Rapid antidepressant-like effect	0:31	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.	0:158	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	3	30	theme	hallucinogenic	496:509	arg1	drug					523:526	a hallucinogenic psychedelic drug	494:526	2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism	461:554	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	31	theme	prelimbic	1136:1144	arg1	region					1146:1151	the prelimbic region	1132:1151	the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1132:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	32	dep	lisuride	558:565	arg1	analog					599:604	non-hallucinogenic psychedelic analog	568:604	non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms	568:638	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	4	33	theme	-treated	960:967	arg1	mice					969:972	LPS (0.5 mg/kg, 23 h before)-treated mice	932:972	LPS (0.5 mg/kg, 23 h before)-treated mice	932:972	Saline (10 ml/kg), DOI (2.0 mg/kg), lisuride (1.0 mg/kg), or (R)-ketamine (10 mg/kg) was administered intraperitoneally to LPS (0.5 mg/kg, 23 h before)-treated mice.
36476377	0	34	theme	non-hallucinogenic	36:53	arg1	lisuride					74:81	non-hallucinogenic psychedelic analog lisuride	36:81	non-hallucinogenic psychedelic analog lisuride	36:81	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	5	35	theme	decreased	1095:1103	arg1	density					1121:1127	the decreased dendritic spine density	1091:1127	the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1091:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	36	dep	DOI	456:458	arg1	2,5-dimethoxy-4-iodoamphetamine					461:491	2,5-dimethoxy-4-iodoamphetamine	461:491	2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism	461:554	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	37	theme	potent	533:538	arg1	agonism					548:554	potent 5-HT2AR agonism	533:554	potent 5-HT2AR agonism	533:554	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	38	with	analog	599:604	arg1	agonisms					631:638	5-HT2AR and 5-HT1AR agonisms	611:638	5-HT2AR and 5-HT1AR agonisms	611:638	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	39	theme	spine	1115:1119	arg1	density					1121:1127	the decreased dendritic spine density	1091:1127	the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1091:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	40	dep	2,5-dimethoxy-4-iodoamphetamine	461:491	arg1	drug					523:526	a hallucinogenic psychedelic drug	494:526	2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism	461:554	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	8	41	theme	rapid-acting	1531:1542	arg1	actions					1559:1565	rapid-acting antidepressant actions	1531:1565	rapid-acting antidepressant actions of psychedelics	1531:1581	It is, therefore, unlikely that 5-HT2AR may play a major role in rapid-acting antidepressant actions of psychedelics although further detailed study is needed.
36476377	1	42	contain	have	238:241	arg2	actions					264:270	rapid antidepressant actions	243:270	rapid antidepressant actions	243:270	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	1	42	contain	have	238:241	arg1	psychedelics					170:181	Classical psychedelics	160:181	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	160:236	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	8	43	theme	psychedelics	1570:1581	arg1	actions					1559:1565	rapid-acting antidepressant actions	1531:1565	rapid-acting antidepressant actions of psychedelics	1531:1581	It is, therefore, unlikely that 5-HT2AR may play a major role in rapid-acting antidepressant actions of psychedelics although further detailed study is needed.
36476377	7	44	theme	5-HT2AR-related	1429:1443	arg1	effects					1457:1463	5-HT2AR-related psychedelic effects	1429:1463	5-HT2AR-related psychedelic effects	1429:1463	This study suggests that antidepressant-like effect of lisuride in LPS-treated mice is not associated with 5-HT2AR-related psychedelic effects.
36476377	8	45	theme	further	1592:1598	arg1	study					1609:1613	further detailed study	1592:1613	further detailed study	1592:1613	It is, therefore, unlikely that 5-HT2AR may play a major role in rapid-acting antidepressant actions of psychedelics although further detailed study is needed.
36476377	3	46	theme	dendritic	730:738	arg1	density					746:752	the decreased dendritic spine density	716:752	the decreased dendritic spine density	716:752	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	47	theme	psychedelic	587:597	arg1	analog					599:604	non-hallucinogenic psychedelic analog	568:604	non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms	568:638	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	48	theme	prefrontal	1163:1172	arg1	cortex					1174:1179	medial prefrontal cortex	1156:1179	medial prefrontal cortex	1156:1179	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	2	49	theme	antidepressant-like	369:387	arg1	actions					389:395	the antidepressant-like actions	365:395	the antidepressant-like actions of psychedelics	365:411	However, there is an ongoing debate over the role of 5-HT2AR in the antidepressant-like actions of psychedelics.
36476377	3	50	with	drug	523:526	arg1	agonism					548:554	potent 5-HT2AR agonism	533:554	potent 5-HT2AR agonism	533:554	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	0	51	theme	analog	67:72	arg1	lisuride					74:81	non-hallucinogenic psychedelic analog lisuride	36:81	non-hallucinogenic psychedelic analog lisuride	36:81	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	7	52	theme	antidepressant-like	1347:1365	arg1	effect					1367:1372	antidepressant-like effect	1347:1372	antidepressant-like effect of lisuride in LPS-treated mice	1347:1404	This study suggests that antidepressant-like effect of lisuride in LPS-treated mice is not associated with 5-HT2AR-related psychedelic effects.
36476377	1	53	theme	antidepressant	249:262	arg1	actions					264:270	rapid antidepressant actions	243:270	rapid antidepressant actions	243:270	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	5	54	theme	immobility	1046:1055	arg1	time					1057:1060	the increased immobility time	1032:1060	the increased immobility time of forced swimming test	1032:1084	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	4	55	theme	R	871:871	arg1	-ketamine					873:881	(R)-ketamine	870:881	(R)-ketamine (10 mg/kg)	870:892	Saline (10 ml/kg), DOI (2.0 mg/kg), lisuride (1.0 mg/kg), or (R)-ketamine (10 mg/kg) was administered intraperitoneally to LPS (0.5 mg/kg, 23 h before)-treated mice.
36476377	4	55	theme	R	871:871	arg1	10 mg/kg					884:891	10 mg/kg	884:891	10 mg/kg	884:891	Saline (10 ml/kg), DOI (2.0 mg/kg), lisuride (1.0 mg/kg), or (R)-ketamine (10 mg/kg) was administered intraperitoneally to LPS (0.5 mg/kg, 23 h before)-treated mice.
36476377	5	56	theme	LPS-treated	1222:1232	arg1	mice					1234:1237	LPS-treated mice	1222:1237	LPS-treated mice	1222:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	57	theme	mice	803:806	arg1	brain					761:765	the brain	757:765	the brain of lipopolysaccharide (LPS)-treated mice	757:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	58	theme	antidepressant	656:669	arg1	-ketamine					674:682	the novel antidepressant (R)-ketamine	646:682	the novel antidepressant (R)-ketamine	646:682	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	59	theme	gyrus	1198:1202	arg1	region					1146:1151	the prelimbic region	1132:1151	the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1132:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	6	60	theme	LPS	1302:1304	arg1	administration					1306:1319	LPS administration	1302:1319	LPS administration	1302:1319	In contrast, DOI did not improve these changes produced after LPS administration.
36476377	0	61	theme	hallucinogenic	92:105	arg1	DOI					119:121	hallucinogenic psychedelic DOI	92:121	hallucinogenic psychedelic DOI	92:121	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	1	62	theme	5-hydroxytryptamine-2A	188:209	arg1	agonism					230:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	0	63	theme	lipopolysaccharide-treated	127:152	arg1	mice					154:157	lipopolysaccharide-treated mice	127:157	lipopolysaccharide-treated mice	127:157	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	5	64	theme	hippocampus	1207:1217	arg1	CA3					1182:1184	CA3	1182:1184	CA3	1182:1184	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	5	64	theme	hippocampus	1207:1217	arg1	gyrus					1198:1202	dentate gyrus	1190:1202	dentate gyrus	1190:1202	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	5	64	theme	hippocampus	1207:1217	arg1	cortex					1174:1179	medial prefrontal cortex	1156:1179	medial prefrontal cortex	1156:1179	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	0	65	theme	DOI	119:121	arg1	effect					26:31	Rapid antidepressant-like effect	0:31	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.	0:158	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	3	66	from	effects	445:451	arg1	behavior					703:710	depression-like behavior	687:710	depression-like behavior	687:710	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	66	from	effects	445:451	arg1	density					746:752	the decreased dendritic spine density	716:752	the decreased dendritic spine density	716:752	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	1	67	with	psychedelics	170:181	arg1	agonism					230:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	5-hydroxytryptamine-2A receptor (5-HT2AR) agonism	188:236	Classical psychedelics with 5-hydroxytryptamine-2A receptor (5-HT2AR) agonism have rapid antidepressant actions in patients with depression.
36476377	2	68	from	role	346:349	arg1	actions					389:395	the antidepressant-like actions	365:395	the antidepressant-like actions of psychedelics	365:411	However, there is an ongoing debate over the role of 5-HT2AR in the antidepressant-like actions of psychedelics.
36476377	4	69	theme	LPS	932:934	arg1	mice					969:972	LPS (0.5 mg/kg, 23 h before)-treated mice	932:972	LPS (0.5 mg/kg, 23 h before)-treated mice	932:972	Saline (10 ml/kg), DOI (2.0 mg/kg), lisuride (1.0 mg/kg), or (R)-ketamine (10 mg/kg) was administered intraperitoneally to LPS (0.5 mg/kg, 23 h before)-treated mice.
36476377	0	70	theme	antidepressant-like	6:24	arg1	effect					26:31	Rapid antidepressant-like effect	0:31	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.	0:158	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	5	71	theme	swimming	1072:1079	arg1	test					1081:1084	forced swimming test	1065:1084	forced swimming test	1065:1084	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	72	theme	DOI	456:458	arg1	effects					445:451	the effects	441:451	the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice	441:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	73	from	time	1057:1060	arg1	region					1146:1151	the prelimbic region	1132:1151	the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1132:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	8	74	theme	major	1517:1521	arg1	role					1523:1526	a major role	1515:1526	a major role	1515:1526	It is, therefore, unlikely that 5-HT2AR may play a major role in rapid-acting antidepressant actions of psychedelics although further detailed study is needed.
36476377	3	75	theme	-ketamine	674:682	arg1	effects					445:451	the effects	441:451	the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice	441:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	76	theme	psychedelic	511:521	arg1	drug					523:526	a hallucinogenic psychedelic drug	494:526	2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism	461:554	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	0	77	theme	psychedelic	55:65	arg1	lisuride					74:81	non-hallucinogenic psychedelic analog lisuride	36:81	non-hallucinogenic psychedelic analog lisuride	36:81	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	3	78	theme	R	672:672	arg1	-ketamine					674:682	the novel antidepressant (R)-ketamine	646:682	the novel antidepressant (R)-ketamine	646:682	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	0	79	theme	lisuride	74:81	arg1	effect					26:31	Rapid antidepressant-like effect	0:31	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.	0:158	Rapid antidepressant-like effect of non-hallucinogenic psychedelic analog lisuride, but not hallucinogenic psychedelic DOI, in lipopolysaccharide-treated mice.
36476377	5	80	theme	dendritic	1105:1113	arg1	density					1121:1127	the decreased dendritic spine density	1091:1127	the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1091:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	2	81	theme	psychedelics	400:411	arg1	actions					389:395	the antidepressant-like actions	365:395	the antidepressant-like actions of psychedelics	365:411	However, there is an ongoing debate over the role of 5-HT2AR in the antidepressant-like actions of psychedelics.
36476377	8	82	theme	antidepressant	1544:1557	arg1	actions					1559:1565	rapid-acting antidepressant actions	1531:1565	rapid-acting antidepressant actions of psychedelics	1531:1581	It is, therefore, unlikely that 5-HT2AR may play a major role in rapid-acting antidepressant actions of psychedelics although further detailed study is needed.
36476377	3	83	theme	5-HT2AR	540:546	arg1	agonism					548:554	potent 5-HT2AR agonism	533:554	potent 5-HT2AR agonism	533:554	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	84	theme	depression-like	687:701	arg1	behavior					703:710	depression-like behavior	687:710	depression-like behavior	687:710	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	2	85	theme	5-HT2AR	354:360	arg1	role					346:349	the role	342:349	the role of 5-HT2AR in the antidepressant-like actions of psychedelics	342:411	However, there is an ongoing debate over the role of 5-HT2AR in the antidepressant-like actions of psychedelics.
36476377	5	86	theme	CA3	1182:1184	arg1	region					1146:1151	the prelimbic region	1132:1151	the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice	1132:1237	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36476377	3	87	from	behavior	703:710	arg1	brain					761:765	the brain	757:765	the brain of lipopolysaccharide (LPS)-treated mice	757:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	88	theme	spine	740:744	arg1	density					746:752	the decreased dendritic spine density	716:752	the decreased dendritic spine density	716:752	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	3	89	theme	lisuride	558:565	arg1	effects					445:451	the effects	441:451	the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice	441:806	In this study, we compared the effects of DOI (2,5-dimethoxy-4-iodoamphetamine: a hallucinogenic psychedelic drug with potent 5-HT2AR agonism), lisuride (non-hallucinogenic psychedelic analog with 5-HT2AR and 5-HT1AR agonisms), and the novel antidepressant (R)-ketamine on depression-like behavior and the decreased dendritic spine density in the brain of lipopolysaccharide (LPS)-treated mice.
36476377	5	90	theme	dentate	1190:1196	arg1	gyrus					1198:1202	dentate gyrus	1190:1202	dentate gyrus	1190:1202	Both lisuride and (R)-ketamine significantly ameliorated the increased immobility time of forced swimming test, and the decreased dendritic spine density in the prelimbic region of medial prefrontal cortex, CA3 and dentate gyrus of hippocampus of LPS-treated mice.
36091223	1	0	dep	times	318:322	arg1	h					336:336	0-72 h	331:336	0-72 h corresponding to LP-0 through LP-72, respectively	331:386	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	5	1	from	LPs	927:929	arg1	analysis					867:874	analysis	867:874	analysis of the monosaccharide composition in the fermented LPs	867:929	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	6	2	theme	worse	1213:1217	arg1	activities					1239:1248	comparable or worse probiotic promoting activities	1199:1248	comparable or worse probiotic promoting activities	1199:1248	Further, LP-6 demonstrated the highest growth for Bifidobacterium compared to LP-0, while the other fermentation time led to comparable or worse probiotic promoting activities.
36091223	2	3	theme	molecular	474:482	arg1	weights					484:490	molecular weights	474:490	molecular weights	474:490	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	5	4	from	analysis	867:874	arg1	LPs					927:929	the fermented LPs	913:929	the fermented LPs	913:929	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	1	5	theme	probiotic	174:182	arg1	potential					184:192	probiotic potential	174:192	probiotic potential	174:192	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	5	6	theme	acid	1017:1020	arg1	proportions					1022:1032	arabinose and galacturonic acid proportions	990:1032	arabinose and galacturonic acid proportions	990:1032	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	0	7	theme	different	105:113	arg1	times					115:119	different times	105:119	different times	105:119	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	0	8	from	Variation	0:8	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	0	8	from	Variation	0:8	arg1	pulp					86:89	litchi pulp	79:89	litchi pulp	79:89	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	3	9	theme	LPs	537:539	arg1	yields					541:546	The LPs yields	533:546	The LPs yields	533:546	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	3	10	theme	uronic	552:557	arg1	contents					564:571	uronic acid contents	552:571	uronic acid contents	552:571	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	1	11	dep	h	336:336	arg1	i.e.					325:328	i.e.	325:328	i.e.	325:328	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	7	12	theme	fermentation	1396:1407	arg1	time					1409:1412	suitable fermentation time	1387:1412	suitable fermentation time	1387:1412	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	6	13	theme	promoting	1229:1237	arg1	activities					1239:1248	comparable or worse probiotic promoting activities	1199:1248	comparable or worse probiotic promoting activities	1199:1248	Further, LP-6 demonstrated the highest growth for Bifidobacterium compared to LP-0, while the other fermentation time led to comparable or worse probiotic promoting activities.
36091223	4	14	contain	contained	733:741	arg2	kDa					840:842	104 kDa	836:842	104 kDa	836:842	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	14	contain	contained	733:741	arg1	LP-6					728:731	LP-6	728:731	LP-6	728:731	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	14	contain	contained	733:741	arg2	yields					766:771	the highest extraction yields	743:771	the highest extraction yields (2.67%)	743:779	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	14	contain	contained	733:741	arg2	%					778:778	2.67%	774:778	2.67%	774:778	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	14	contain	contained	733:741	arg2	contents					801:808	lowest uronic acid contents	782:808	lowest uronic acid contents	782:808	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	14	contain	contained	733:741	arg2	<					848:848	p < 0.05	846:853	p < 0.05	846:853	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	14	contain	contained	733:741	arg2	Mw					832:833	smallest average Mw	815:833	smallest average Mw (104 kDa) (p < 0.05)	815:854	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	7	15	theme	probiotic	1432:1440	arg1	activities					1442:1451	their probiotic activities	1426:1451	their probiotic activities	1426:1451	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	5	16	theme	unfermented	1056:1066	arg1	LP-0					1068:1071	unfermented LP-0	1056:1071	unfermented LP-0	1056:1071	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	4	17	theme	average	824:830	arg1	Mw					832:833	smallest average Mw	815:833	smallest average Mw (104 kDa) (p < 0.05)	815:854	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	17	theme	average	824:830	arg1	kDa					840:842	104 kDa	836:842	104 kDa	836:842	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	17	theme	average	824:830	arg1	<					848:848	p < 0.05	846:853	p < 0.05	846:853	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	5	18	theme	fermented	917:925	arg1	LPs					927:929	the fermented LPs	913:929	the fermented LPs	913:929	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	7	19	theme	acid	1285:1288	arg1	bacteria					1290:1297	lactic acid bacteria	1278:1297	lactic acid bacteria fermentation	1278:1310	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	2	20	theme	monosaccharide	497:510	arg1	compositions					512:523	monosaccharide compositions	497:523	monosaccharide compositions	497:523	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	0	21	from	activities	44:53	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	0	21	from	activities	44:53	arg1	pulp					86:89	litchi pulp	79:89	litchi pulp	79:89	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	4	22	theme	smallest	815:822	arg1	Mw					832:833	smallest average Mw	815:833	smallest average Mw (104 kDa) (p < 0.05)	815:854	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	22	theme	smallest	815:822	arg1	kDa					840:842	104 kDa	836:842	104 kDa	836:842	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	22	theme	smallest	815:822	arg1	<					848:848	p < 0.05	846:853	p < 0.05	846:853	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	6	23	theme	highest	1105:1111	arg1	growth					1113:1118	the highest growth	1101:1118	the highest growth for Bifidobacterium	1101:1138	Further, LP-6 demonstrated the highest growth for Bifidobacterium compared to LP-0, while the other fermentation time led to comparable or worse probiotic promoting activities.
36091223	2	24	theme	Fermentation	390:401	arg1	times					403:407	Fermentation times	390:407	Fermentation times	390:407	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	3	25	theme	sugar	651:655	arg1	contents					657:664	total sugar contents	645:664	total sugar contents	645:664	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	3	26	with	association	603:613	arg1	time					633:636	fermentation time	620:636	fermentation time	620:636	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	3	27	theme	acid	559:562	arg1	contents					564:571	uronic acid contents	552:571	uronic acid contents	552:571	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	1	28	theme	different	197:205	arg1	LPs					224:226	LPs	224:226	LPs	224:226	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	1	28	theme	different	197:205	arg1	polysaccharides					207:221	different polysaccharides	197:221	different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively)	197:387	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	6	29	theme	comparable	1199:1208	arg1	activities					1239:1248	comparable or worse probiotic promoting activities	1199:1248	comparable or worse probiotic promoting activities	1199:1248	Further, LP-6 demonstrated the highest growth for Bifidobacterium compared to LP-0, while the other fermentation time led to comparable or worse probiotic promoting activities.
36091223	6	30	theme	probiotic	1219:1227	arg1	activities					1239:1248	comparable or worse probiotic promoting activities	1199:1248	comparable or worse probiotic promoting activities	1199:1248	Further, LP-6 demonstrated the highest growth for Bifidobacterium compared to LP-0, while the other fermentation time led to comparable or worse probiotic promoting activities.
36091223	1	31	theme	polysaccharides	207:221	arg1	structures					159:168	chemical structures	150:168	chemical structures	150:168	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	1	31	theme	polysaccharides	207:221	arg1	potential					184:192	probiotic potential	174:192	probiotic potential	174:192	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	2	32	theme	sugar	436:440	arg1	contents					442:449	total sugar contents	430:449	total sugar contents	430:449	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	4	33	theme	uronic	789:794	arg1	contents					801:808	lowest uronic acid contents	782:808	lowest uronic acid contents	782:808	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	6	34	theme	fermentation	1174:1185	arg1	time					1187:1190	the other fermentation time	1164:1190	the other fermentation time	1164:1190	Further, LP-6 demonstrated the highest growth for Bifidobacterium compared to LP-0, while the other fermentation time led to comparable or worse probiotic promoting activities.
36091223	4	35	theme	extraction	755:764	arg1	yields					766:771	the highest extraction yields	743:771	the highest extraction yields (2.67%)	743:779	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	35	theme	extraction	755:764	arg1	%					778:778	2.67%	774:778	2.67%	774:778	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	5	36	theme	composition	898:908	arg1	analysis					867:874	analysis	867:874	analysis of the monosaccharide composition in the fermented LPs	867:929	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	2	37	theme	total	430:434	arg1	contents					442:449	total sugar contents	430:449	total sugar contents	430:449	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	1	38	theme	different	308:316	arg1	times					318:322	different times	308:322	different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively)	308:387	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	3	39	theme	total	645:649	arg1	contents					657:664	total sugar contents	645:664	total sugar contents	645:664	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	1	40	dep	structures	159:168	arg1	the					146:148	the	146:148	the	146:148	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	3	41	from	trends	593:598	arg1	association					603:613	association	603:613	association with fermentation time	603:636	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	7	42	theme	lactic	1278:1283	arg1	bacteria					1290:1297	lactic acid bacteria	1278:1297	lactic acid bacteria fermentation	1278:1310	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	0	43	from	pulp	86:89	arg1	activities					44:53	probiotic activities	34:53	probiotic activities of polysaccharides from litchi pulp	34:89	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	0	43	from	pulp	86:89	arg1	Variation					0:8	Variation	0:8	Variation in characterization	0:28	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	0	43	from	pulp	86:89	arg1	polysaccharides					58:72	polysaccharides	58:72	polysaccharides from litchi pulp	58:89	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	7	44	theme	bacteria	1290:1297	arg1	fermentation					1299:1310	lactic acid bacteria fermentation	1278:1310	lactic acid bacteria fermentation	1278:1310	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	7	45	dep	alters	1312:1317	arg1	enhance					1418:1424	enhance	1418:1424	can enhance their probiotic activities	1414:1451	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	0	46	theme	probiotic	34:42	arg1	activities					44:53	probiotic activities	34:53	probiotic activities of polysaccharides from litchi pulp	34:89	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	4	47	theme	acid	796:799	arg1	contents					801:808	lowest uronic acid contents	782:808	lowest uronic acid contents	782:808	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	7	48	theme	polysaccharides	1360:1374	arg1	properties					1339:1348	the physicochemical properties	1319:1348	the physicochemical properties of litchi polysaccharides	1319:1374	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	0	49	theme	polysaccharides	58:72	arg1	activities					44:53	probiotic activities	34:53	probiotic activities of polysaccharides from litchi pulp	34:89	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	0	49	theme	polysaccharides	58:72	arg1	Variation					0:8	Variation	0:8	Variation in characterization	0:28	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	2	50	theme	LPs	528:530	arg1	contents					442:449	total sugar contents	430:449	total sugar contents	430:449	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	2	50	theme	LPs	528:530	arg1	contents					464:471	uronic acid contents	452:471	uronic acid contents	452:471	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	2	50	theme	LPs	528:530	arg1	yields					422:427	the yields	418:427	the yields	418:427	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	2	50	theme	LPs	528:530	arg1	weights					484:490	molecular weights	474:490	molecular weights	474:490	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	2	50	theme	LPs	528:530	arg1	compositions					512:523	monosaccharide compositions	497:523	monosaccharide compositions	497:523	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	7	51	theme	litchi	1353:1358	arg1	polysaccharides					1360:1374	litchi polysaccharides	1353:1374	litchi polysaccharides	1353:1374	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	2	52	theme	uronic	452:457	arg1	contents					464:471	uronic acid contents	452:471	uronic acid contents	452:471	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	4	53	theme	p	846:846	arg1	Mw					832:833	smallest average Mw	815:833	smallest average Mw (104 kDa) (p < 0.05)	815:854	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	53	theme	p	846:846	arg1	<					848:848	p < 0.05	846:853	p < 0.05	846:853	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	3	54	theme	irregular	583:591	arg1	trends					593:598	irregular trends	583:598	irregular trends in association with fermentation time	583:636	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	7	55	dep	enhance	1418:1424	arg1	such					1377:1380	such	1377:1380	such	1377:1380	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	7	56	theme	physicochemical	1323:1337	arg1	properties					1339:1348	the physicochemical properties	1319:1348	the physicochemical properties of litchi polysaccharides	1319:1374	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	5	57	theme	arabinose	990:998	arg1	proportions					1022:1032	arabinose and galacturonic acid proportions	990:1032	arabinose and galacturonic acid proportions	990:1032	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	3	58	theme	fermentation	620:631	arg1	time					633:636	fermentation time	620:636	fermentation time	620:636	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	5	59	theme	glucose	965:971	arg1	proportions					950:960	the proportions	946:960	the proportions of glucose	946:971	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	5	60	theme	monosaccharide	883:896	arg1	composition					898:908	the monosaccharide composition	879:908	the monosaccharide composition in the fermented LPs	879:929	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	7	61	theme	suitable	1387:1394	arg1	time					1409:1412	suitable fermentation time	1387:1412	suitable fermentation time	1387:1412	These results suggest that lactic acid bacteria fermentation alters the physicochemical properties of litchi polysaccharides, such that suitable fermentation time can enhance their probiotic activities.
36091223	4	62	theme	highest	747:753	arg1	yields					766:771	the highest extraction yields	743:771	the highest extraction yields (2.67%)	743:779	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	4	62	theme	highest	747:753	arg1	%					778:778	2.67%	774:778	2.67%	774:778	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	0	63	theme	litchi	79:84	arg1	pulp					86:89	litchi pulp	79:89	litchi pulp	79:89	Variation in characterization and probiotic activities of polysaccharides from litchi pulp fermented for different times.
36091223	5	64	theme	galacturonic	1004:1015	arg1	acid					1017:1020	galacturonic acid	1004:1020	galacturonic acid	1004:1020	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	3	65	theme	molecular	685:693	arg1	weights					695:701	the molecular weights	681:701	the molecular weights	681:701	The LPs yields and uronic acid contents exhibited irregular trends in association with fermentation time, while total sugar contents decreased, and the molecular weights increased.
36091223	1	66	theme	chemical	150:157	arg1	structures					159:168	chemical structures	150:168	chemical structures	150:168	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	5	67	from	composition	898:908	arg1	LPs					927:929	the fermented LPs	913:929	the fermented LPs	913:929	Moreover, analysis of the monosaccharide composition in the fermented LPs indicated that the proportions of glucose decreased, while arabinose and galacturonic acid proportions increased relative to unfermented LP-0.
36091223	1	68	theme	litchi	248:253	arg1	pulp					255:258	the litchi pulp	244:258	the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively)	244:387	This study investigated the chemical structures and probiotic potential of different polysaccharides (LPs) extracted from the litchi pulp that fermented with Lactobacillus fermentum for different times (i.e., 0-72 h corresponding to LP-0 through LP-72, respectively).
36091223	2	69	theme	acid	459:462	arg1	contents					464:471	uronic acid contents	452:471	uronic acid contents	452:471	Fermentation times affected the yields, total sugar contents, uronic acid contents, molecular weights, and monosaccharide compositions of LPs.
36091223	4	70	theme	lowest	782:787	arg1	contents					801:808	lowest uronic acid contents	782:808	lowest uronic acid contents	782:808	Particularly, LP-6 contained the highest extraction yields (2.67%), lowest uronic acid contents, and smallest average Mw (104 kDa) (p < 0.05).
36091223	6	71	theme	other	1168:1172	arg1	time					1187:1190	the other fermentation time	1164:1190	the other fermentation time	1164:1190	Further, LP-6 demonstrated the highest growth for Bifidobacterium compared to LP-0, while the other fermentation time led to comparable or worse probiotic promoting activities.
35666767	11	0	theme	flake	1605:1609	arg1	density					1611:1617	flake density	1605:1617	flake density	1605:1617	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	14	1	theme	heat-sealed	1996:2006	arg1	bags					2013:2016	heat-sealed foil bags	1996:2016	heat-sealed foil bags	1996:2016	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	5	2	dep	°C	807:808	arg1	=					831:831	=	831:831	=	831:831	In experiment 3, sifted flakes (335 g/L) were stored for 3-d at either 23 °C (starch availability = 55%) or 55 °C to induce starch retrogradation (starch availability = 41%).
35666767	8	3	contain	had	1146:1148	arg1	corn					1141:1144	whole shelled corn	1127:1144	whole shelled corn	1127:1144	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	8	3	contain	had	1146:1148	arg2	<					1160:1160	P < 0.01	1158:1165	P < 0.01	1158:1165	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	8	3	contain	had	1146:1148	arg2	solubility					1176:1185	lesser (P < 0.01) ruminal solubility	1150:1185	lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn	1150:1237	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	12	4	theme	sifted	1766:1771	arg1	fines					1773:1777	sifted fines	1766:1777	sifted fines	1766:1777	The soluble DM fraction of sifted fines tended to decrease (P = 0.06) linearly with increasing flake density.
35666767	6	5	theme	nylon	972:976	arg1	bags					978:981	nylon bags	972:981	nylon bags	972:981	All samples for each of the three experiments were weighed into nylon bags and ruminally incubated for 0-h to estimate the soluble fraction.
35666767	14	6	dep	decreased	2035:2043	arg1	<					2048:2048	P < 0.04	2046:2053	P < 0.04	2046:2053	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	13	7	theme	flake	1936:1940	arg1	density					1942:1948	flake density	1936:1948	flake density	1936:1948	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	16	8	theme	flakes	2296:2301	arg1	solubility					2282:2291	ruminal solubility	2274:2291	ruminal solubility of flakes	2274:2301	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	4	9	theme	sifted	685:690	arg1	flakes					692:697	sifted flakes	685:697	sifted flakes (>4 mm)	685:705	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	4	9	theme	sifted	685:690	arg1	mm					703:704	>4 mm	700:704	>4 mm	700:704	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	14	10	theme	S	2118:2118	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	11	11	dep	increasing	1594:1603	arg1	decreased					1628:1636	decreased	1628:1636	increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1594:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	17	12	theme	Inducing	2405:2412	arg1	retrogradation					2421:2434	Inducing starch retrogradation	2405:2434	Inducing starch retrogradation	2405:2434	Inducing starch retrogradation decreases ruminal solubility of starch, nonstarch OM, and minerals.
35666767	13	13	theme	Zn	1876:1877	arg1	fractions					1887:1895	Total starch, CP, NDF, and Zn soluble fractions	1849:1895	Total starch, CP, NDF, and Zn soluble fractions of sifted fines	1849:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	1	14	theme	body	162:165	arg1	weight					167:172	body weight	162:172	body weight = 390 ± 7.86 kg	162:188	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	14	15	theme	sifted	1979:1984	arg1	flakes					1986:1991	sifted flakes	1979:1991	sifted flakes	1979:1991	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	2	16	theme	1-mm	451:454	arg1	screen					456:461	a 1-mm screen	449:461	a 1-mm screen	449:461	In experiment 1, corn was left whole or processed with no screen, ground through a 6-mm screen, or ground through a 1-mm screen.
35666767	12	17	theme	flake	1834:1838	arg1	density					1840:1846	flake density	1834:1846	flake density	1834:1846	The soluble DM fraction of sifted fines tended to decrease (P = 0.06) linearly with increasing flake density.
35666767	6	18	theme	soluble	1031:1037	arg1	fraction					1039:1046	the soluble fraction	1027:1046	the soluble fraction	1027:1046	All samples for each of the three experiments were weighed into nylon bags and ruminally incubated for 0-h to estimate the soluble fraction.
35666767	5	19	theme	starch	811:816	arg1	availability					818:829	starch availability	811:829	starch availability	811:829	In experiment 3, sifted flakes (335 g/L) were stored for 3-d at either 23 °C (starch availability = 55%) or 55 °C to induce starch retrogradation (starch availability = 41%).
35666767	15	20	theme	nutrients	2225:2233	arg1	solubility					2211:2220	the solubility	2207:2220	the solubility of nutrients	2207:2233	With each increase in the degree of corn processing, there was an increase in the solubility of nutrients.
35666767	1	21	theme	=	174:174	arg1	weight					167:172	body weight	162:172	body weight = 390 ± 7.86 kg	162:188	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	15	22	theme	corn	2165:2168	arg1	processing					2170:2179	corn processing	2165:2179	corn processing	2165:2179	With each increase in the degree of corn processing, there was an increase in the solubility of nutrients.
35666767	9	23	contain	had	1279:1281	arg2	solubility					1310:1319	greater (P < 0.01) ruminal solubility	1283:1319	greater (P < 0.01) ruminal solubility of all nutrients measured	1283:1345	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	9	23	contain	had	1279:1281	arg1	ground					1245:1250	Corn ground	1240:1250	Corn ground with a screen (6 and 1 mm)	1240:1277	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	17	24	theme	ruminal	2446:2452	arg1	solubility					2454:2463	ruminal solubility	2446:2463	ruminal solubility of starch, nonstarch OM, and minerals	2446:2501	Inducing starch retrogradation decreases ruminal solubility of starch, nonstarch OM, and minerals.
35666767	10	25	theme	greater	1427:1433	arg1	solubility					1454:1463	greater (P < 0.03) ruminal solubility	1427:1463	greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn	1427:1526	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	1	26	theme	±	180:180	arg1	kg					187:188	390 ± 7.86 kg	176:188	390 ± 7.86 kg	176:188	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	8	27	theme	lesser	1150:1155	arg1	<					1160:1160	P < 0.01	1158:1165	P < 0.01	1158:1165	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	8	27	theme	lesser	1150:1155	arg1	solubility					1176:1185	lesser (P < 0.01) ruminal solubility	1150:1185	lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn	1150:1237	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	14	28	theme	total	2085:2089	arg1	starch					2091:2096	total starch	2085:2096	total starch	2085:2096	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	0	29	theme	minerals	118:125	arg1	solubility					76:85	ruminal solubility	68:85	ruminal solubility of starch, fiber, protein, and minerals	68:125	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	8	30	theme	P	1158:1158	arg1	<					1160:1160	P < 0.01	1158:1165	P < 0.01	1158:1165	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	8	30	theme	P	1158:1158	arg1	solubility					1176:1185	lesser (P < 0.01) ruminal solubility	1150:1185	lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn	1150:1237	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	14	31	theme	DM	2081:2082	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	13	32	theme	Total	1849:1853	arg1	fractions					1887:1895	Total starch, CP, NDF, and Zn soluble fractions	1849:1895	Total starch, CP, NDF, and Zn soluble fractions of sifted fines	1849:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	11	33	theme	starch	1683:1688	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	14	34	from	°C	2024:2025	arg1	storage					1968:1974	storage	1968:1974	storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d	1968:2033	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	1	35	theme	density	268:274	arg1	effects					234:240	effects	234:240	effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation	234:332	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	10	36	theme	P	1436:1436	arg1	<					1438:1438	P < 0.03	1436:1443	P < 0.03	1436:1443	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	8	37	theme	ruminal	1168:1174	arg1	<					1160:1160	P < 0.01	1158:1165	P < 0.01	1158:1165	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	8	37	theme	ruminal	1168:1174	arg1	solubility					1176:1185	lesser (P < 0.01) ruminal solubility	1150:1185	lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn	1150:1237	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	14	38	theme	K	2115:2115	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	0	39	theme	ruminal	68:74	arg1	solubility					76:85	ruminal solubility	68:85	ruminal solubility of starch, fiber, protein, and minerals	68:125	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	13	40	theme	sifted	1900:1905	arg1	fines					1907:1911	sifted fines	1900:1911	sifted fines	1900:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	11	41	theme	ADF	1695:1697	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	2	42	from	whole	366:370	arg1	experiment					338:347	experiment 1	338:349	experiment 1	338:349	In experiment 1, corn was left whole or processed with no screen, ground through a 6-mm screen, or ground through a 1-mm screen.
35666767	16	43	theme	flake	2247:2251	arg1	density					2253:2259	Increasing flake density	2236:2259	Increasing flake density	2236:2259	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	1	44	theme	retrogradation	288:301	arg1	effects					234:240	effects	234:240	effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation	234:332	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	5	45	theme	sifted	750:755	arg1	flakes					757:762	sifted flakes	750:762	sifted flakes (335 g/L)	750:772	In experiment 3, sifted flakes (335 g/L) were stored for 3-d at either 23 °C (starch availability = 55%) or 55 °C to induce starch retrogradation (starch availability = 41%).
35666767	5	45	theme	sifted	750:755	arg1	g/L					769:771	335 g/L	765:771	335 g/L	765:771	In experiment 3, sifted flakes (335 g/L) were stored for 3-d at either 23 °C (starch availability = 55%) or 55 °C to induce starch retrogradation (starch availability = 41%).
35666767	11	46	theme	CP	1691:1692	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	8	47	theme	nutrients	1194:1202	arg1	<					1160:1160	P < 0.01	1158:1165	P < 0.01	1158:1165	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	8	47	theme	nutrients	1194:1202	arg1	solubility					1176:1185	lesser (P < 0.01) ruminal solubility	1150:1185	lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn	1150:1237	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	4	48	theme	4-mm	616:619	arg1	screen					621:626	a 4-mm screen	614:626	a 4-mm screen to produce two particle sizes within each flake density	614:682	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	17	49	theme	nonstarch	2476:2484	arg1	OM					2486:2487	nonstarch OM	2476:2487	nonstarch OM	2476:2487	Inducing starch retrogradation decreases ruminal solubility of starch, nonstarch OM, and minerals.
35666767	9	50	with	ground	1366:1371	arg1	screen					1381:1386	no screen	1378:1386	no screen	1378:1386	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	1	51	theme	in	306:307	arg1	degradation					322:332	in situ ruminal degradation	306:332	in situ ruminal degradation	306:332	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	16	52	theme	flake	2390:2394	arg1	density					2396:2402	flake density	2390:2402	flake density	2390:2402	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	9	53	theme	Corn	1240:1243	arg1	ground					1245:1250	Corn ground	1240:1250	Corn ground with a screen (6 and 1 mm)	1240:1277	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	1	54	dep	=	174:174	arg1	kg					187:188	390 ± 7.86 kg	176:188	390 ± 7.86 kg	176:188	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	0	55	theme	Corn	0:3	arg1	processing					5:14	Corn processing	0:14	Corn processing	0:14	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	13	56	theme	NDF	1867:1869	arg1	fractions					1887:1895	Total starch, CP, NDF, and Zn soluble fractions	1849:1895	Total starch, CP, NDF, and Zn soluble fractions of sifted fines	1849:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	8	57	theme	ground	1227:1232	arg1	corn					1234:1237	ground corn	1227:1237	ground corn	1227:1237	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	17	58	theme	minerals	2494:2501	arg1	solubility					2454:2463	ruminal solubility	2446:2463	ruminal solubility of starch, nonstarch OM, and minerals	2446:2501	Inducing starch retrogradation decreases ruminal solubility of starch, nonstarch OM, and minerals.
35666767	14	59	from	storage	1968:1974	arg1	bags					2013:2016	heat-sealed foil bags	1996:2016	heat-sealed foil bags	1996:2016	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	1	60	theme	corn	245:248	arg1	processing					250:259	corn processing	245:259	corn processing	245:259	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	2	61	theme	no	390:391	arg1	screen					393:398	no screen	390:398	no screen	390:398	In experiment 1, corn was left whole or processed with no screen, ground through a 6-mm screen, or ground through a 1-mm screen.
35666767	11	62	theme	soluble	1653:1659	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	10	63	theme	ruminal	1446:1452	arg1	solubility					1454:1463	greater (P < 0.03) ruminal solubility	1427:1463	greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn	1427:1526	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	5	64	theme	starch	880:885	arg1	availability					887:898	starch availability	880:898	starch availability	880:898	In experiment 3, sifted flakes (335 g/L) were stored for 3-d at either 23 °C (starch availability = 55%) or 55 °C to induce starch retrogradation (starch availability = 41%).
35666767	4	65	theme	flake	670:674	arg1	density					676:682	each flake density	665:682	each flake density	665:682	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	16	66	theme	soluble	2317:2323	arg1	fraction					2325:2332	the soluble fraction	2313:2332	the soluble fraction of sifted fines	2313:2348	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	16	66	theme	soluble	2317:2323	arg1	much					2371:2374	much	2371:2374	much	2371:2374	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	15	67	from	increase	2195:2202	arg1	solubility					2211:2220	the solubility	2207:2220	the solubility of nutrients	2207:2233	With each increase in the degree of corn processing, there was an increase in the solubility of nutrients.
35666767	12	68	theme	fines	1773:1777	arg1	fraction					1754:1761	The soluble DM fraction	1739:1761	The soluble DM fraction of sifted fines	1739:1777	The soluble DM fraction of sifted fines tended to decrease (P = 0.06) linearly with increasing flake density.
35666767	8	69	theme	shelled	1133:1139	arg1	corn					1141:1144	whole shelled corn	1127:1144	whole shelled corn	1127:1144	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
35666767	9	70	theme	greater	1283:1289	arg1	solubility					1310:1319	greater (P < 0.01) ruminal solubility	1283:1319	greater (P < 0.01) ruminal solubility of all nutrients measured	1283:1345	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	14	71	theme	foil	2008:2011	arg1	bags					2013:2016	heat-sealed foil bags	1996:2016	heat-sealed foil bags	1996:2016	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	16	72	theme	Increasing	2236:2245	arg1	density					2253:2259	Increasing flake density	2236:2259	Increasing flake density	2236:2259	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	13	73	theme	soluble	1879:1885	arg1	fractions					1887:1895	Total starch, CP, NDF, and Zn soluble fractions	1849:1895	Total starch, CP, NDF, and Zn soluble fractions of sifted fines	1849:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	16	74	theme	fines	2344:2348	arg1	fraction					2325:2332	the soluble fraction	2313:2332	the soluble fraction of sifted fines	2313:2348	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	16	74	theme	fines	2344:2348	arg1	much					2371:2374	much	2371:2374	much	2371:2374	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	9	75	theme	P	1292:1292	arg1	<					1294:1294	P < 0.01	1292:1299	P < 0.01	1292:1299	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	13	76	theme	starch	1855:1860	arg1	fractions					1887:1895	Total starch, CP, NDF, and Zn soluble fractions	1849:1895	Total starch, CP, NDF, and Zn soluble fractions of sifted fines	1849:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	11	77	theme	flakes	1731:1736	arg1	starch					1683:1688	total starch	1677:1688	total starch	1677:1688	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	AHF					1700:1702	AHF	1700:1702	AHF	1700:1702	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	P					1705:1705	P	1705:1705	P	1705:1705	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	Zn					1718:1719	Zn	1718:1719	Zn	1718:1719	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	CP					1691:1692	CP	1691:1692	CP	1691:1692	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	DM					1673:1674	DM	1673:1674	DM	1673:1674	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	ADF					1695:1697	ADF	1695:1697	ADF	1695:1697	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	K					1708:1708	K	1708:1708	K	1708:1708	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	11	77	theme	flakes	1731:1736	arg1	S					1711:1711	S	1711:1711	S	1711:1711	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	12	78	theme	DM	1751:1752	arg1	fraction					1754:1761	The soluble DM fraction	1739:1761	The soluble DM fraction of sifted fines	1739:1777	The soluble DM fraction of sifted fines tended to decrease (P = 0.06) linearly with increasing flake density.
35666767	15	79	theme	processing	2170:2179	arg1	degree					2155:2160	the degree	2151:2160	the degree of corn processing	2151:2179	With each increase in the degree of corn processing, there was an increase in the solubility of nutrients.
35666767	10	80	theme	Corn	1389:1392	arg1	ground					1394:1399	Corn ground	1389:1399	Corn ground through a 1-mm screen	1389:1421	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	14	81	theme	Mg	2111:2112	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	11	82	theme	P	1705:1705	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	1	83	from	effects	234:240	arg1	degradation					322:332	in situ ruminal degradation	306:332	in situ ruminal degradation	306:332	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	16	84	theme	ruminal	2274:2280	arg1	solubility					2282:2291	ruminal solubility	2274:2291	ruminal solubility of flakes	2274:2301	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	1	85	theme	cannulated	143:152	arg1	steers					154:159	Five ruminally cannulated steers	128:159	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg)	128:189	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	9	86	theme	ruminal	1302:1308	arg1	solubility					1310:1319	greater (P < 0.01) ruminal solubility	1283:1319	greater (P < 0.01) ruminal solubility of all nutrients measured	1283:1345	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	14	87	theme	P	2108:2108	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	11	88	theme	AHF	1700:1702	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	10	89	theme	1-mm	1411:1414	arg1	screen					1416:1421	a 1-mm screen	1409:1421	a 1-mm screen	1409:1421	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	14	90	theme	flakes	1986:1991	arg1	storage					1968:1974	storage	1968:1974	storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d	1968:2033	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	14	91	theme	NDF	2103:2105	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	11	92	theme	S	1711:1711	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	9	93	with	ground	1245:1250	arg1	mm					1275:1276	6 and 1 mm	1267:1276	6 and 1 mm	1267:1276	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	9	93	with	ground	1245:1250	arg1	screen					1259:1264	a screen	1257:1264	a screen (6 and 1 mm)	1257:1277	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	17	94	theme	starch	2414:2419	arg1	retrogradation					2421:2434	Inducing starch retrogradation	2405:2434	Inducing starch retrogradation	2405:2434	Inducing starch retrogradation decreases ruminal solubility of starch, nonstarch OM, and minerals.
35666767	0	95	theme	protein	105:111	arg1	solubility					76:85	ruminal solubility	68:85	ruminal solubility of starch, fiber, protein, and minerals	68:125	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	9	96	theme	nutrients	1328:1336	arg1	solubility					1310:1319	greater (P < 0.01) ruminal solubility	1283:1319	greater (P < 0.01) ruminal solubility of all nutrients measured	1283:1345	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	14	97	theme	CP	2099:2100	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	11	98	theme	K	1708:1708	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	9	99	dep	greater	1283:1289	arg1	<					1294:1294	P < 0.01	1292:1299	P < 0.01	1292:1299	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	13	100	theme	CP	1863:1864	arg1	fractions					1887:1895	Total starch, CP, NDF, and Zn soluble fractions	1849:1895	Total starch, CP, NDF, and Zn soluble fractions of sifted fines	1849:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	10	101	theme	corn	1542:1545	arg1	ground					1547:1552	corn ground	1542:1552	corn ground through a 6-mm screen	1542:1574	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	12	102	theme	soluble	1743:1749	arg1	fraction					1754:1761	The soluble DM fraction	1739:1761	The soluble DM fraction of sifted fines	1739:1777	The soluble DM fraction of sifted fines tended to decrease (P = 0.06) linearly with increasing flake density.
35666767	7	103	theme	nutrient	1088:1095	arg1	composition					1097:1107	nutrient composition	1088:1107	nutrient composition	1088:1107	The residue remaining was analyzed for nutrient composition.
35666767	10	104	dep	greater	1427:1433	arg1	<					1438:1438	P < 0.03	1436:1443	P < 0.03	1436:1443	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	5	105	theme	starch	857:862	arg1	retrogradation					864:877	starch retrogradation	857:877	starch retrogradation	857:877	In experiment 3, sifted flakes (335 g/L) were stored for 3-d at either 23 °C (starch availability = 55%) or 55 °C to induce starch retrogradation (starch availability = 41%).
35666767	9	106	theme	corn	1361:1364	arg1	ground					1366:1371	corn ground	1361:1371	corn ground with no screen	1361:1386	Corn ground with a screen (6 and 1 mm) had greater (P < 0.01) ruminal solubility of all nutrients measured compared with corn ground with no screen.
35666767	17	107	theme	starch	2468:2473	arg1	solubility					2454:2463	ruminal solubility	2446:2463	ruminal solubility of starch, nonstarch OM, and minerals	2446:2501	Inducing starch retrogradation decreases ruminal solubility of starch, nonstarch OM, and minerals.
35666767	4	108	theme	sifted	711:716	arg1	fines					718:722	sifted fines	711:722	sifted fines (<4 mm)	711:730	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	4	108	theme	sifted	711:716	arg1	mm					728:729	<4 mm	725:729	<4 mm	725:729	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	7	109	theme	remaining	1061:1069	arg1	residue					1053:1059	The residue remaining	1049:1069	The residue remaining	1049:1069	The residue remaining was analyzed for nutrient composition.
35666767	4	110	theme	flake	569:573	arg1	densities					575:583	These four flake densities	558:583	These four flake densities	558:583	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	3	111	theme	steam-flaked	493:504	arg1	corn					506:509	steam-flaked corn	493:509	steam-flaked corn	493:509	In experiment 2, we produced steam-flaked corn at four densities: 309, 335, 360, and 386 g/L.
35666767	11	112	theme	sifted	1724:1729	arg1	flakes					1731:1736	sifted flakes	1724:1736	sifted flakes	1724:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	14	113	theme	soluble	2060:2066	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	14	114	theme	Fe	2125:2126	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	11	115	theme	total	1677:1681	arg1	starch					1683:1688	total starch	1677:1688	total starch	1677:1688	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	1	116	theme	ruminal	314:320	arg1	degradation					322:332	in situ ruminal degradation	306:332	in situ ruminal degradation	306:332	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	0	117	theme	starch	90:95	arg1	solubility					76:85	ruminal solubility	68:85	ruminal solubility of starch, fiber, protein, and minerals	68:125	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	1	118	theme	starch	281:286	arg1	retrogradation					288:301	starch retrogradation	281:301	starch retrogradation	281:301	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	0	119	theme	fiber	98:102	arg1	solubility					76:85	ruminal solubility	68:85	ruminal solubility of starch, fiber, protein, and minerals	68:125	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	15	120	from	increase	2139:2146	arg1	degree					2155:2160	the degree	2151:2160	the degree of corn processing	2151:2179	With each increase in the degree of corn processing, there was an increase in the solubility of nutrients.
35666767	2	121	theme	6-mm	418:421	arg1	screen					423:428	a 6-mm screen	416:428	a 6-mm screen	416:428	In experiment 1, corn was left whole or processed with no screen, ground through a 6-mm screen, or ground through a 1-mm screen.
35666767	11	122	theme	P	1639:1639	arg1	<					1641:1641	P < 0.02	1639:1646	P < 0.02	1639:1646	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	17	123	theme	OM	2486:2487	arg1	solubility					2454:2463	ruminal solubility	2446:2463	ruminal solubility of starch, nonstarch OM, and minerals	2446:2501	Inducing starch retrogradation decreases ruminal solubility of starch, nonstarch OM, and minerals.
35666767	10	124	contain	had	1423:1425	arg2	solubility					1454:1463	greater (P < 0.03) ruminal solubility	1427:1463	greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn	1427:1526	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	10	124	contain	had	1423:1425	arg1	ground					1394:1399	Corn ground	1389:1399	Corn ground through a 1-mm screen	1389:1421	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	0	125	theme	flake	17:21	arg1	density					23:29	flake density	17:29	flake density	17:29	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	14	126	theme	P	2046:2046	arg1	<					2048:2048	P < 0.04	2046:2053	P < 0.04	2046:2053	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	10	127	theme	DM	1468:1469	arg1	solubility					1454:1463	greater (P < 0.03) ruminal solubility	1427:1463	greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn	1427:1526	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	13	128	theme	fines	1907:1911	arg1	fractions					1887:1895	Total starch, CP, NDF, and Zn soluble fractions	1849:1895	Total starch, CP, NDF, and Zn soluble fractions of sifted fines	1849:1911	Total starch, CP, NDF, and Zn soluble fractions of sifted fines were not influenced by flake density.
35666767	11	129	theme	DM	1673:1674	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	1	130	dep	in	306:307	arg1	situ					309:312	situ	309:312	situ	309:312	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	11	131	dep	decreased	1628:1636	arg1	<					1641:1641	P < 0.02	1639:1646	P < 0.02	1639:1646	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	4	132	theme	particle	643:650	arg1	sizes					652:656	two particle sizes	639:656	two particle sizes within each flake density	639:682	These four flake densities were sifted for 20 s through a 4-mm screen to produce two particle sizes within each flake density: sifted flakes (>4 mm) and sifted fines (<4 mm).
35666767	0	133	theme	starch	36:41	arg1	retrogradation					43:56	starch retrogradation	36:56	starch retrogradation	36:56	Corn processing, flake density, and starch retrogradation influence ruminal solubility of starch, fiber, protein, and minerals.
35666767	1	134	theme	processing	250:259	arg1	effects					234:240	effects	234:240	effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation	234:332	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	1	135	used	used	196:199	arg2	steers					154:159	Five ruminally cannulated steers	128:159	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg)	128:189	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	16	136	from	changes	2379:2385	arg1	density					2396:2402	flake density	2390:2402	flake density	2390:2402	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	1	137	dep	steers	154:159	arg1	weight					167:172	body weight	162:172	body weight = 390 ± 7.86 kg	162:188	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	1	138	theme	flake	262:266	arg1	density					268:274	flake density	262:274	flake density	262:274	Five ruminally cannulated steers (body weight = 390 ± 7.86 kg) were used in three experiments to evaluate effects of corn processing, flake density, and starch retrogradation on in situ ruminal degradation.
35666767	14	139	theme	starch	2091:2096	arg1	fractions					2068:2076	the soluble fractions	2056:2076	the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe	2056:2126	In experiment 3, storage of sifted flakes in heat-sealed foil bags at 55 °C for 3-d decreased (P < 0.04) the soluble fractions of DM, total starch, CP, NDF, P, Mg, K, S, and Fe.
35666767	10	140	theme	6-mm	1564:1567	arg1	screen					1569:1574	a 6-mm screen	1562:1574	a 6-mm screen	1562:1574	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	10	141	theme	total	1472:1476	arg1	DM					1468:1469	DM	1468:1469	DM	1468:1469	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	10	141	theme	total	1472:1476	arg1	starch					1478:1483	total starch	1472:1483	total starch	1472:1483	Corn ground through a 1-mm screen had greater (P < 0.03) ruminal solubility of DM, total starch, CP, ADF, AHF, P, Mg, K, S, Zn, Fe, and Mn compared with corn ground through a 6-mm screen.
35666767	16	142	theme	sifted	2337:2342	arg1	fines					2344:2348	sifted fines	2337:2348	sifted fines	2337:2348	Increasing flake density can decrease ruminal solubility of flakes; however, the soluble fraction of sifted fines is not influenced as much by changes in flake density.
35666767	2	143	from	experiment	338:347	arg1	whole					366:370	whole	366:370	whole	366:370	In experiment 1, corn was left whole or processed with no screen, ground through a 6-mm screen, or ground through a 1-mm screen.
35666767	11	144	theme	Zn	1718:1719	arg1	fraction					1661:1668	the soluble fraction	1649:1668	the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes	1649:1736	In experiment 2, increasing flake density linearly decreased (P < 0.02) the soluble fraction of DM, total starch, CP, ADF, AHF, P, K, S, and Zn of sifted flakes.
35666767	8	145	theme	whole	1127:1131	arg1	corn					1141:1144	whole shelled corn	1127:1144	whole shelled corn	1127:1144	In experiment 1, whole shelled corn had lesser (P < 0.01) ruminal solubility of all nutrients measured compared with ground corn.
36752462	4	0	theme	previous	633:640	arg1	infarction					653:662	previous myocardial infarction	633:662	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	1	dep	METHODS	522:528	arg1	This					530:533	This	530:533	This	530:533	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	12	2	from	VT	1418:1419	arg1	inducibility					1366:1377	VT inducibility	1363:1377	VT inducibility	1363:1377	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	12	2	from	VT	1418:1419	arg1	freedom					1405:1411	freedom	1405:1411	freedom from VT (P = 0.013)	1405:1431	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	12	2	from	VT	1418:1419	arg1	predictor					1392:1400	the only predictor	1383:1400	the only predictor of freedom from VT (P = 0.013)	1383:1431	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	16	3	dep	Registry	1921:1928	arg1	NCT03649022					1931:1941	NCT03649022	1931:1941	NCT03649022	1931:1941	(Ventricular Tachycardia Ablation Registry; NCT03649022).
36752462	3	4	dep	OBJECTIVES	349:358	arg1	evaluated					371:379	evaluated	371:379	evaluated a homogenous simplified catheter ablation strategy for different substrates	371:455	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	3	4	dep	OBJECTIVES	349:358	arg1	compared					461:468	compared	461:468	compared the results with those of a single referral center	461:519	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	2	5	theme	ablation	328:335	arg1	strategies					337:346	ablation strategies	328:346	ablation strategies	328:346	This difference could be related to heterogeneous mapping and ablation strategies.
36752462	10	6	from	baseline	1263:1270	arg1	LPs					1256:1258	LPs	1256:1258	LPs at baseline	1256:1270	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	4	7	theme	dilated	745:751	arg1	cardiomyopathy					753:766	idiopathic dilated cardiomyopathy	734:766	idiopathic dilated cardiomyopathy	734:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	15	8	theme	cardiomyopathy	1871:1884	arg1	causes					1861:1866	4 causes	1859:1866	4 causes of cardiomyopathy	1859:1884	LP abolition and noninducibility were effective in reducing VT recurrences in patients with 4 causes of cardiomyopathy.
36752462	4	9	theme	right	702:706	arg1	dysplasia					720:728	arrhythmogenic right ventricular dysplasia	687:728	arrhythmogenic right ventricular dysplasia	687:728	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	10	with	patients	589:596	arg1	causes					619:624	the following 4 causes	603:624	the following 4 causes of VT	603:630	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	5	11	theme	VT	920:921	arg1	abolition					887:895	late potential (LP) abolition	867:895	late potential (LP) abolition	867:895	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	5	11	theme	VT	920:921	arg1	LP					883:884	LP	883:884	LP	883:884	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	5	11	theme	VT	920:921	arg1	noninducibility					901:915	noninducibility	901:915	noninducibility of VT	901:921	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	13	12	with	patients	1437:1444	arg1	LPs					1451:1453	LPs	1451:1453	LPs	1451:1453	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	12	13	theme	study	1350:1354	arg1	group					1356:1360	the overall study group	1338:1360	the overall study group	1338:1360	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	14	14	theme	VT	1634:1635	arg1	CONCLUSIONS					1607:1617	CONCLUSIONS	1607:1617	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.	1607:1765	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	14	14	theme	VT	1634:1635	arg1	mapping					1637:1643	A standardized VT mapping and ablation technique	1619:1666	mapping	1637:1643	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	13	15	theme	complete	1512:1519	arg1	P = 0.040					1535:1543	P = 0.040	1535:1543	P = 0.040	1535:1543	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	13	15	theme	complete	1512:1519	arg1	abolition					1524:1532	complete LP abolition	1512:1532	complete LP abolition (P = 0.040)	1512:1544	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	1	16	theme	ventricular	152:162	arg1	tachycardia					164:174	BACKGROUND Multicenter ventricular tachycardia	129:174	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies	129:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	1	16	theme	ventricular	152:162	arg1	VT					177:178	VT	177:178	VT	177:178	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	5	17	theme	procedural	773:782	arg1	protocol					784:791	The procedural protocol	769:791	The procedural protocol	769:791	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	3	18	theme	homogenous	383:392	arg1	strategy					423:430	a homogenous simplified catheter ablation strategy	381:430	a homogenous simplified catheter ablation strategy for different substrates	381:455	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	5	19	theme	combined	846:853	arg1	endpoint					855:862	the combined endpoint	842:862	the combined endpoint of late potential (LP) abolition and noninducibility of VT	842:921	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	4	20	theme	VT	565:566	arg1	METHODS					522:528	METHODS	522:528	METHODS This	522:533	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	20	theme	VT	565:566	arg1	registry					577:584	a multicenter prospective VT ablation registry	539:584	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	0	21	theme	Italian	78:84	arg1	Registry					119:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	11	22	from	12 months	1292:1300	arg1	Freedom					1273:1279	Freedom	1273:1279	Freedom from VT at 12 months	1273:1300	Freedom from VT at 12 months was observed in 67% of patients.
36752462	0	23	theme	Tachycardia Ablation	98:117	arg1	Registry					119:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	12	24	from	predictor	1392:1400	arg1	P = 0.013					1422:1430	P = 0.013	1422:1430	P = 0.013	1422:1430	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	12	24	from	predictor	1392:1400	arg1	group					1356:1360	the overall study group	1338:1360	the overall study group	1338:1360	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	12	24	from	predictor	1392:1400	arg1	VT					1418:1419	VT	1418:1419	VT (P = 0.013)	1418:1431	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	3	25	theme	catheter	405:412	arg1	strategy					423:430	a homogenous simplified catheter ablation strategy	381:430	a homogenous simplified catheter ablation strategy for different substrates	381:455	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	15	26	theme	LP	1767:1768	arg1	abolition					1770:1778	LP abolition	1767:1778	LP abolition	1767:1778	LP abolition and noninducibility were effective in reducing VT recurrences in patients with 4 causes of cardiomyopathy.
36752462	3	27	theme	single	498:503	arg1	center					514:519	a single referral center	496:519	a single referral center	496:519	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	13	28	with	patients	1498:1505	arg1	P = 0.040					1535:1543	P = 0.040	1535:1543	P = 0.040	1535:1543	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	13	28	with	patients	1498:1505	arg1	abolition					1524:1532	complete LP abolition	1512:1532	complete LP abolition (P = 0.040)	1512:1544	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	7	29	theme	patients	1008:1015	arg1	total					995:999	A total	993:999	RESULTS A total of 309 patients	985:1015	RESULTS A total of 309 patients were enrolled.
36752462	4	30	theme	multicenter	541:551	arg1	METHODS					522:528	METHODS	522:528	METHODS This	522:533	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	30	theme	multicenter	541:551	arg1	registry					577:584	a multicenter prospective VT ablation registry	539:584	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	13	31	theme	VT	1460:1461	arg1	lower					1483:1487	lower	1483:1487	lower	1483:1487	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	13	31	theme	VT	1460:1461	arg1	rate					1474:1477	the VT recurrence rate	1456:1477	the VT recurrence rate	1456:1477	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	5	32	theme	abolition	887:895	arg1	endpoint					855:862	the combined endpoint	842:862	the combined endpoint of late potential (LP) abolition and noninducibility of VT	842:921	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	10	33	theme	primary	1157:1163	arg1	endpoint					1174:1181	The primary combined endpoint	1153:1181	The primary combined endpoint of LP abolition and noninducibility	1153:1217	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	3	34	theme	different	436:444	arg1	substrates					446:455	different substrates	436:455	different substrates	436:455	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	5	35	theme	noninducibility	901:915	arg1	endpoint					855:862	the combined endpoint	842:862	the combined endpoint of late potential (LP) abolition and noninducibility of VT	842:921	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	1	36	theme	BACKGROUND	129:138	arg1	tachycardia					164:174	BACKGROUND Multicenter ventricular tachycardia	129:174	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies	129:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	1	36	theme	BACKGROUND	129:138	arg1	VT					177:178	VT	177:178	VT	177:178	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	14	37	dep	CONCLUSIONS	1607:1617	arg1	CONCLUSIONS					1607:1617	CONCLUSIONS	1607:1617	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.	1607:1765	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	14	37	dep	CONCLUSIONS	1607:1617	arg1	mapping					1637:1643	A standardized VT mapping and ablation technique	1619:1666	mapping	1637:1643	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	14	37	dep	CONCLUSIONS	1607:1617	arg1	technique					1658:1666	A standardized VT mapping and ablation technique	1619:1666	technique	1658:1666	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	0	38	theme	Long-Term	31:39	arg1	Outcome					41:47	Long-Term Outcome	31:47	Long-Term Outcome	31:47	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	10	39	theme	LP	1186:1187	arg1	abolition					1189:1197	LP abolition	1186:1197	LP abolition	1186:1197	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	5	40	theme	potential	872:880	arg1	abolition					887:895	late potential (LP) abolition	867:895	late potential (LP) abolition	867:895	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	5	40	theme	potential	872:880	arg1	LP					883:884	LP	883:884	LP	883:884	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	6	41	theme	primary	938:944	arg1	freedom					968:974	freedom	968:974	freedom from VT	968:982	The long-term primary efficacy endpoint was freedom from VT.
36752462	6	41	theme	primary	938:944	arg1	endpoint					955:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint was freedom from VT.
36752462	4	42	theme	patients	589:596	arg1	METHODS					522:528	METHODS	522:528	METHODS This	522:533	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	42	theme	patients	589:596	arg1	registry					577:584	a multicenter prospective VT ablation registry	539:584	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	0	43	theme	Prospective	54:64	arg1	Registry					119:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	16	44	theme	Ventricular	1888:1898	arg1	Registry					1921:1928	Ventricular Tachycardia Ablation Registry	1888:1928	Ventricular Tachycardia Ablation Registry; NCT03649022	1888:1941	(Ventricular Tachycardia Ablation Registry; NCT03649022).
36752462	13	45	theme	composite	1575:1583	arg1	P = 0.035					1595:1603	P = 0.035	1595:1603	P = 0.035	1595:1603	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	13	45	theme	composite	1575:1583	arg1	endpoint					1585:1592	the composite endpoint	1571:1592	the composite endpoint (P = 0.035)	1571:1604	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	11	46	located	observed	1306:1313	arg2	Freedom					1273:1279	Freedom	1273:1279	Freedom from VT at 12 months	1273:1300	Freedom from VT at 12 months was observed in 67% of patients.
36752462	11	46	located	observed	1306:1313	arg1	patients					1325:1332	patients	1325:1332	patients	1325:1332	Freedom from VT at 12 months was observed in 67% of patients.
36752462	11	46	located	observed	1306:1313	arg1	%					1320:1320	67%	1318:1320	67% of patients	1318:1332	Freedom from VT at 12 months was observed in 67% of patients.
36752462	14	47	theme	referral	1715:1722	arg1	center					1724:1729	a single referral center	1706:1729	a single referral center	1706:1729	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	16	48	theme	Ablation	1912:1919	arg1	Registry					1921:1928	Ventricular Tachycardia Ablation Registry	1888:1928	Ventricular Tachycardia Ablation Registry; NCT03649022	1888:1941	(Ventricular Tachycardia Ablation Registry; NCT03649022).
36752462	8	49	from	present	1041:1047	arg1	patients					1059:1066	patients	1059:1066	patients	1059:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	8	49	from	present	1041:1047	arg1	%					1054:1054	70%	1052:1054	70% of patients	1052:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	0	50	dep	Matching	0:7	arg1	Registry					119:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	11	51	from	VT	1286:1287	arg1	Freedom					1273:1279	Freedom	1273:1279	Freedom from VT at 12 months	1273:1300	Freedom from VT at 12 months was observed in 67% of patients.
36752462	7	52	dep	RESULTS	985:991	arg1	total					995:999	A total	993:999	RESULTS A total of 309 patients	985:1015	RESULTS A total of 309 patients were enrolled.
36752462	12	53	theme	freedom	1405:1411	arg1	inducibility					1366:1377	VT inducibility	1363:1377	VT inducibility	1363:1377	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	12	53	theme	freedom	1405:1411	arg1	predictor					1392:1400	the only predictor	1383:1400	the only predictor of freedom from VT (P = 0.013)	1383:1431	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	4	54	theme	myocardial	642:651	arg1	infarction					653:662	previous myocardial infarction	633:662	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	6	55	from	VT	981:982	arg1	freedom					968:974	freedom	968:974	freedom from VT	968:982	The long-term primary efficacy endpoint was freedom from VT.
36752462	6	55	from	VT	981:982	arg1	endpoint					955:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint was freedom from VT.
36752462	8	56	theme	patients	1059:1066	arg1	patients					1059:1066	patients	1059:1066	patients	1059:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	8	56	theme	patients	1059:1066	arg1	%					1054:1054	70%	1052:1054	70% of patients	1052:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	12	57	theme	only	1387:1390	arg1	inducibility					1366:1377	VT inducibility	1363:1377	VT inducibility	1363:1377	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	12	57	theme	only	1387:1390	arg1	predictor					1392:1400	the only predictor	1383:1400	the only predictor of freedom from VT (P = 0.013)	1383:1431	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	11	58	theme	patients	1325:1332	arg1	patients					1325:1332	patients	1325:1332	patients	1325:1332	Freedom from VT at 12 months was observed in 67% of patients.
36752462	11	58	theme	patients	1325:1332	arg1	%					1320:1320	67%	1318:1320	67% of patients	1318:1332	Freedom from VT at 12 months was observed in 67% of patients.
36752462	13	59	theme	LP	1521:1522	arg1	P = 0.040					1535:1543	P = 0.040	1535:1543	P = 0.040	1535:1543	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	13	59	theme	LP	1521:1522	arg1	abolition					1524:1532	complete LP abolition	1512:1532	complete LP abolition (P = 0.040)	1512:1544	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	1	60	theme	poorer	209:214	arg1	outcomes					216:223	poorer outcomes	209:223	poorer outcomes	209:223	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	4	61	theme	idiopathic	734:743	arg1	cardiomyopathy					753:766	idiopathic dilated cardiomyopathy	734:766	idiopathic dilated cardiomyopathy	734:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	12	62	theme	VT	1363:1364	arg1	inducibility					1366:1377	VT inducibility	1363:1377	VT inducibility	1363:1377	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	12	62	theme	VT	1363:1364	arg1	predictor					1392:1400	the only predictor	1383:1400	the only predictor of freedom from VT (P = 0.013)	1383:1431	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	4	63	theme	arrhythmogenic	687:700	arg1	dysplasia					720:728	arrhythmogenic right ventricular dysplasia	687:728	arrhythmogenic right ventricular dysplasia	687:728	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	5	64	theme	precise	802:808	arg1	mapping					810:816	precise mapping	802:816	precise mapping	802:816	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	14	65	theme	ablation	1649:1656	arg1	CONCLUSIONS					1607:1617	CONCLUSIONS	1607:1617	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.	1607:1765	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	14	65	theme	ablation	1649:1656	arg1	technique					1658:1666	A standardized VT mapping and ablation technique	1619:1666	technique	1658:1666	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	1	66	theme	single-center	239:251	arg1	experiences					253:263	single-center experiences	239:263	single-center experiences	239:263	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	4	67	theme	ventricular	708:718	arg1	dysplasia					720:728	arrhythmogenic right ventricular dysplasia	687:728	arrhythmogenic right ventricular dysplasia	687:728	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	1	68	theme	Multicenter	140:150	arg1	tachycardia					164:174	BACKGROUND Multicenter ventricular tachycardia	129:174	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies	129:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	1	68	theme	Multicenter	140:150	arg1	VT					177:178	VT	177:178	VT	177:178	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	12	69	theme	overall	1342:1348	arg1	group					1356:1360	the overall study group	1338:1360	the overall study group	1338:1360	In the overall study group, VT inducibility was the only predictor of freedom from VT (P = 0.013).
36752462	14	70	theme	standardized	1621:1632	arg1	CONCLUSIONS					1607:1617	CONCLUSIONS	1607:1617	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.	1607:1765	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	14	70	theme	standardized	1621:1632	arg1	mapping					1637:1643	A standardized VT mapping and ablation technique	1619:1666	mapping	1637:1643	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	2	71	theme	heterogeneous	302:314	arg1	mapping					316:322	heterogeneous mapping	302:322	heterogeneous mapping	302:322	This difference could be related to heterogeneous mapping and ablation strategies.
36752462	1	72	theme	tachycardia	164:174	arg1	studies					190:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies	129:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies	129:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	8	73	attach	present	1041:1047	arg1	patients					1059:1066	patients	1059:1066	patients	1059:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	8	73	attach	present	1041:1047	arg2	LPs					1032:1034	LPs	1032:1034	LPs	1032:1034	LPs were present in 70% of patients and were abolished in 83%.
36752462	8	73	attach	present	1041:1047	arg1	%					1054:1054	70%	1052:1054	70% of patients	1052:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	10	74	theme	patients	1242:1249	arg1	patients					1242:1249	patients	1242:1249	patients with LPs at baseline	1242:1270	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	10	74	theme	patients	1242:1249	arg1	%					1237:1237	64%	1235:1237	64% of patients with LPs at baseline	1235:1270	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	0	75	theme	Multicenter	66:76	arg1	Registry					119:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	3	76	theme	simplified	394:403	arg1	strategy					423:430	a homogenous simplified catheter ablation strategy	381:430	a homogenous simplified catheter ablation strategy for different substrates	381:455	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	4	77	theme	ablation	568:575	arg1	METHODS					522:528	METHODS	522:528	METHODS This	522:533	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	77	theme	ablation	568:575	arg1	registry					577:584	a multicenter prospective VT ablation registry	539:584	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	78	theme	previous	665:672	arg1	myocarditis					674:684	previous myocarditis	665:684	previous myocarditis	665:684	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	5	79	theme	ablation	822:829	arg1	steps					831:835	ablation steps	822:835	ablation steps	822:835	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	0	80	theme	Ventricular	86:96	arg1	Registry					119:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry	50:126	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	1	81	theme	ablation	181:188	arg1	studies					190:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies	129:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies	129:196	BACKGROUND Multicenter ventricular tachycardia (VT) ablation studies have shown poorer outcomes compared with single-center experiences.
36752462	9	82	from	end	1102:1104	arg1	noninducible					1139:1150	noninducible	1139:1150	noninducible	1139:1150	At the end of the procedure 74% of LPs were noninducible.
36752462	14	83	from	outcomes	1694:1701	arg1	study					1760:1764	a multicenter prospective study	1734:1764	a multicenter prospective study	1734:1764	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	15	84	with	patients	1845:1852	arg1	causes					1861:1866	4 causes	1859:1866	4 causes of cardiomyopathy	1859:1884	LP abolition and noninducibility were effective in reducing VT recurrences in patients with 4 causes of cardiomyopathy.
36752462	3	85	theme	ablation	414:421	arg1	strategy					423:430	a homogenous simplified catheter ablation strategy	381:430	a homogenous simplified catheter ablation strategy for different substrates	381:455	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	4	86	theme	prospective	553:563	arg1	METHODS					522:528	METHODS	522:528	METHODS This	522:533	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	86	theme	prospective	553:563	arg1	registry					577:584	a multicenter prospective VT ablation registry	539:584	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	14	87	theme	single	1708:1713	arg1	center					1724:1729	a single referral center	1706:1729	a single referral center	1706:1729	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	9	88	theme	procedure	1113:1121	arg1	end					1102:1104	the end	1098:1104	the end of the procedure	1098:1121	At the end of the procedure 74% of LPs were noninducible.
36752462	3	89	theme	referral	505:512	arg1	center					514:519	a single referral center	496:519	a single referral center	496:519	OBJECTIVES This study evaluated a homogenous simplified catheter ablation strategy for different substrates and compared the results with those of a single referral center.
36752462	0	90	theme	Ablation	9:16	arg1	Endpoints					18:26	Ablation Endpoints	9:26	Ablation Endpoints	9:26	Matching Ablation Endpoints to Long-Term Outcome: The Prospective Multicenter Italian Ventricular Tachycardia Ablation Registry.
36752462	13	91	theme	recurrence	1463:1472	arg1	lower					1483:1487	lower	1483:1487	lower	1483:1487	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	13	91	theme	recurrence	1463:1472	arg1	rate					1474:1477	the VT recurrence rate	1456:1477	the VT recurrence rate	1456:1477	In patients with LPs, the VT recurrence rate was lower both for patients with complete LP abolition (P = 0.040) and for patients meeting the composite endpoint (P = 0.035).
36752462	10	92	with	patients	1242:1249	arg1	LPs					1256:1258	LPs	1256:1258	LPs at baseline	1256:1270	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	14	93	theme	procedural	1683:1692	arg1	outcomes					1694:1701	the procedural outcomes	1679:1701	the procedural outcomes of a single referral center in a multicenter prospective study	1679:1764	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	4	94	theme	VT	629:630	arg1	causes					619:624	the following 4 causes	603:624	the following 4 causes of VT	603:630	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	9	95	theme	LPs	1130:1132	arg1	LPs					1130:1132	LPs	1130:1132	LPs	1130:1132	At the end of the procedure 74% of LPs were noninducible.
36752462	9	95	theme	LPs	1130:1132	arg1	%					1125:1125	74%	1123:1125	74% of LPs	1123:1132	At the end of the procedure 74% of LPs were noninducible.
36752462	5	96	theme	late	867:870	arg1	abolition					887:895	late potential (LP) abolition	867:895	late potential (LP) abolition	867:895	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	5	96	theme	late	867:870	arg1	LP					883:884	LP	883:884	LP	883:884	The procedural protocol included precise mapping and ablation steps with the combined endpoint of late potential (LP) abolition and noninducibility of VT.
36752462	14	97	theme	prospective	1748:1758	arg1	study					1760:1764	a multicenter prospective study	1734:1764	a multicenter prospective study	1734:1764	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	6	98	theme	efficacy	946:953	arg1	freedom					968:974	freedom	968:974	freedom from VT	968:982	The long-term primary efficacy endpoint was freedom from VT.
36752462	6	98	theme	efficacy	946:953	arg1	endpoint					955:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint was freedom from VT.
36752462	4	99	dep	registry	577:584	arg1	cardiomyopathy					753:766	idiopathic dilated cardiomyopathy	734:766	idiopathic dilated cardiomyopathy	734:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	99	dep	registry	577:584	arg1	infarction					653:662	previous myocardial infarction	633:662	a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy	539:766	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	99	dep	registry	577:584	arg1	myocarditis					674:684	previous myocarditis	665:684	previous myocarditis	665:684	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	4	99	dep	registry	577:584	arg1	dysplasia					720:728	arrhythmogenic right ventricular dysplasia	687:728	arrhythmogenic right ventricular dysplasia	687:728	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	6	100	theme	long-term	928:936	arg1	freedom					968:974	freedom	968:974	freedom from VT	968:982	The long-term primary efficacy endpoint was freedom from VT.
36752462	6	100	theme	long-term	928:936	arg1	endpoint					955:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint	924:962	The long-term primary efficacy endpoint was freedom from VT.
36752462	16	101	theme	Tachycardia	1900:1910	arg1	Registry					1921:1928	Ventricular Tachycardia Ablation Registry	1888:1928	Ventricular Tachycardia Ablation Registry; NCT03649022	1888:1941	(Ventricular Tachycardia Ablation Registry; NCT03649022).
36752462	8	102	from	%	1054:1054	arg1	present					1041:1047	present	1041:1047	present	1041:1047	LPs were present in 70% of patients and were abolished in 83%.
36752462	4	103	theme	following	607:615	arg1	causes					619:624	the following 4 causes	603:624	the following 4 causes of VT	603:630	METHODS This was a multicenter prospective VT ablation registry of patients with the following 4 causes of VT: previous myocardial infarction; previous myocarditis; arrhythmogenic right ventricular dysplasia; or idiopathic dilated cardiomyopathy.
36752462	10	104	theme	combined	1165:1172	arg1	endpoint					1174:1181	The primary combined endpoint	1153:1181	The primary combined endpoint of LP abolition and noninducibility	1153:1217	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	8	105	located	present	1041:1047	arg1	patients					1059:1066	patients	1059:1066	patients	1059:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	8	105	located	present	1041:1047	arg1	%					1054:1054	70%	1052:1054	70% of patients	1052:1066	LPs were present in 70% of patients and were abolished in 83%.
36752462	8	105	located	present	1041:1047	arg2	LPs					1032:1034	LPs	1032:1034	LPs	1032:1034	LPs were present in 70% of patients and were abolished in 83%.
36752462	10	106	theme	noninducibility	1203:1217	arg1	endpoint					1174:1181	The primary combined endpoint	1153:1181	The primary combined endpoint of LP abolition and noninducibility	1153:1217	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36752462	15	107	theme	VT	1827:1828	arg1	recurrences					1830:1840	VT recurrences	1827:1840	VT recurrences in patients with 4 causes of cardiomyopathy	1827:1884	LP abolition and noninducibility were effective in reducing VT recurrences in patients with 4 causes of cardiomyopathy.
36752462	15	108	from	recurrences	1830:1840	arg1	patients					1845:1852	patients	1845:1852	patients with 4 causes of cardiomyopathy	1845:1884	LP abolition and noninducibility were effective in reducing VT recurrences in patients with 4 causes of cardiomyopathy.
36752462	14	109	theme	center	1724:1729	arg1	outcomes					1694:1701	the procedural outcomes	1679:1701	the procedural outcomes of a single referral center in a multicenter prospective study	1679:1764	CONCLUSIONS A standardized VT mapping and ablation technique reproduced the procedural outcomes of a single referral center in a multicenter prospective study.
36752462	10	110	theme	abolition	1189:1197	arg1	endpoint					1174:1181	The primary combined endpoint	1153:1181	The primary combined endpoint of LP abolition and noninducibility	1153:1217	The primary combined endpoint of LP abolition and noninducibility was achieved in 64% of patients with LPs at baseline.
36550591	11	0	theme	H12-8	1530:1534	arg1	group					1536:1540	H12-8 group	1530:1540	H12-8 group	1530:1540	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	4	1	theme	preference	575:584	arg1	test					586:589	sucrose preference test	567:589	sucrose preference test	567:589	The behaviors were studied by forced swimming test, open field test, sucrose preference test, and body weight.
36550591	5	2	link	linked	737:742	arg1	assay					758:762	enzyme linked immunosorbent assay	730:762	enzyme linked immunosorbent assay	730:762	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	2	3	dep	METHODS	242:248	arg1	cultured					328:335	cultured	328:335	cultured microbiota in vitro	328:355	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	2	3	dep	METHODS	242:248	arg1	collected					253:261	collected	253:261	collected fecal microbiota from patients with depressive disorder (DD)	253:322	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	2	4	theme	fecal	263:267	arg1	microbiota					269:278	fecal microbiota	263:278	fecal microbiota from patients with depressive disorder (DD)	263:322	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	8	5	used	used	974:977	arg2	We					971:972	We	971:972	We	971:972	We used lipopolysaccharide (LPS) to mimic activated rat primary astrocyte in vitro.
36550591	6	6	theme	acidic	782:787	arg1	protein					789:795	Glial fibrillary acidic protein	765:795	Glial fibrillary acidic protein (GFAP)	765:802	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	6	6	theme	acidic	782:787	arg1	biomarker					807:815	a biomarker	805:815	a biomarker of astrocyte	805:828	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	6	6	theme	acidic	782:787	arg1	GFAP					798:801	GFAP	798:801	GFAP	798:801	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	0	7	attach	derived	61:67	arg1	disorder					85:92	depressive disorder	74:92	depressive disorder	74:92	Antidepression of Xingpijieyu formula targets gut microbiota derived from depressive disorder.
36550591	0	7	attach	derived	61:67	arg2	microbiota					50:59	gut microbiota	46:59	gut microbiota derived from depressive disorder	46:92	Antidepression of Xingpijieyu formula targets gut microbiota derived from depressive disorder.
36550591	15	8	from	inflammation	1964:1975	arg1	astrocyte					2012:2020	astrocyte	2012:2020	astrocyte	2012:2020	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	5	9	theme	serum	693:697	arg1	cytokines					699:707	serum cytokines	693:707	serum cytokines	693:707	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	9	10	link	Brain-derived	1055:1067	arg1	BDNF					1090:1093	BDNF	1090:1093	BDNF	1090:1093	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	9	10	link	Brain-derived	1055:1067	arg1	factor					1082:1087	Brain-derived neurotrophic factor	1055:1087	Brain-derived neurotrophic factor (BDNF)	1055:1094	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	15	11	from	activation	1978:1987	arg1	astrocyte					2012:2020	astrocyte	2012:2020	astrocyte	2012:2020	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	14	12	theme	oxidative	1886:1894	arg1	stress					1896:1901	oxidative stress	1886:1901	oxidative stress	1886:1901	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	14	13	link	brain-derived	1816:1828	arg1	BDNF					1851:1854	BDNF	1851:1854	BDNF	1851:1854	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	14	13	link	brain-derived	1816:1828	arg1	factor					1843:1848	brain-derived neurotrophic factor	1816:1848	brain-derived neurotrophic factor (BDNF)	1816:1855	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	11	14	link	DD-derived	1454:1463	arg1	composition					1476:1486	DD-derived microbiota composition	1454:1486	DD-derived microbiota composition	1454:1486	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	1	15	theme	antidepressant	146:159	arg1	role					161:164	the antidepressant role	142:164	the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis	142:239	OBJECTIVE This investigation aims to determine the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis.
36550591	2	16	from	patients	285:292	arg1	microbiota					269:278	fecal microbiota	263:278	fecal microbiota from patients with depressive disorder (DD)	263:322	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	11	17	theme	DD-derived	1454:1463	arg1	composition					1476:1486	DD-derived microbiota composition	1454:1486	DD-derived microbiota composition	1454:1486	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	9	18	theme	triphosphate	1270:1281	arg1	kits					1320:1323	2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits	1227:1323	2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits	1227:1323	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	1	19	dep	OBJECTIVE	95:103	arg1	aims					124:127	aims	124:127	aims to determine the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis	124:239	OBJECTIVE This investigation aims to determine the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis.
36550591	8	20	theme	activated	1013:1021	arg1	astrocyte					1035:1043	activated rat primary astrocyte	1013:1043	activated rat primary astrocyte	1013:1043	We used lipopolysaccharide (LPS) to mimic activated rat primary astrocyte in vitro.
36550591	1	21	theme	formula	181:187	arg1	role					161:164	the antidepressant role	142:164	the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis	142:239	OBJECTIVE This investigation aims to determine the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis.
36550591	9	22	theme	Brain-derived	1055:1067	arg1	BDNF					1090:1093	BDNF	1090:1093	BDNF	1090:1093	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	9	22	theme	Brain-derived	1055:1067	arg1	factor					1082:1087	Brain-derived neurotrophic factor	1055:1087	Brain-derived neurotrophic factor (BDNF)	1055:1094	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	8	23	theme	primary	1027:1033	arg1	astrocyte					1035:1043	activated rat primary astrocyte	1013:1043	activated rat primary astrocyte	1013:1043	We used lipopolysaccharide (LPS) to mimic activated rat primary astrocyte in vitro.
36550591	7	24	theme	RNA	944:946	arg1	technology					959:968	RNA sequencing technology	944:968	RNA sequencing technology	944:968	Microbiota culturing in vitro after XPJYF treatment was analyze by 16 s RNA sequencing technology.
36550591	12	25	theme	altered	1660:1666	arg1	pathways					1668:1675	altered pathways	1660:1675	altered pathways	1660:1675	GLUCOSE1PMETAB-PWY and PWY-7328 of which biofunctions were dominantly encoded by Biffdobacterium were the top two of altered pathways.
36550591	9	26	theme	factor	1082:1087	arg1	factors					1129:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors	1055:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors	1055:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	3	27	theme	intragastric	424:435	arg1	administration					437:450	the intragastric administration	420:450	the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day	420:495	Some of microbiota were transplanted into germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day.
36550591	5	28	theme	linked	737:742	arg1	assay					758:762	enzyme linked immunosorbent assay	730:762	enzyme linked immunosorbent assay	730:762	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	9	29	theme	glucose-1-phosphate	1294:1312	arg1	kits					1320:1323	2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits	1227:1323	2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits	1227:1323	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	15	30	theme	axis	2091:2094	arg1	dysfunction					2096:2106	HPA axis dysfunction	2087:2106	HPA axis dysfunction	2087:2106	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	0	31	theme	depressive	74:83	arg1	disorder					85:92	depressive disorder	74:92	depressive disorder	74:92	Antidepression of Xingpijieyu formula targets gut microbiota derived from depressive disorder.
36550591	3	32	theme	grain	461:465	arg1	administration					437:450	the intragastric administration	420:450	the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day	420:495	Some of microbiota were transplanted into germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day.
36550591	0	33	theme	Xingpijieyu	18:28	arg1	formula					30:36	Xingpijieyu formula	18:36	Xingpijieyu formula	18:36	Antidepression of Xingpijieyu formula targets gut microbiota derived from depressive disorder.
36550591	14	34	theme	brain-derived	1816:1828	arg1	BDNF					1851:1854	BDNF	1851:1854	BDNF	1851:1854	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	14	34	theme	brain-derived	1816:1828	arg1	factor					1843:1848	brain-derived neurotrophic factor	1816:1848	brain-derived neurotrophic factor (BDNF)	1816:1855	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	11	35	from	composition	1476:1486	arg1	enriched					1442:1449	enriched	1442:1449	enriched	1442:1449	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	3	36	theme	germ-free	400:408	arg1	rats					410:413	germ-free rats	400:413	germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day	400:495	Some of microbiota were transplanted into germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day.
36550591	4	37	theme	open	550:553	arg1	test					561:564	open field test	550:564	open field test	550:564	The behaviors were studied by forced swimming test, open field test, sucrose preference test, and body weight.
36550591	15	38	theme	microbiota	2030:2039	arg1	modulation					2041:2050	gut microbiota modulation	2026:2050	gut microbiota modulation	2026:2050	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	15	39	dep	targets	1956:1962	arg1	improve					2061:2067	improve	2061:2067	improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats	2061:2157	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	13	40	from	activation	1727:1736	arg1	rats					1752:1755	depression rats	1741:1755	depression rats	1741:1755	XPJYF improved behaviors and repressed astrocyte activation in depression rats.
36550591	9	41	theme	oxidative	1112:1120	arg1	stress					1122:1127	oxidative stress	1112:1127	oxidative stress	1112:1127	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	11	42	from	enriched	1442:1449	arg1	Lactobacillus					1513:1525	Lactobacillus	1513:1525	Lactobacillus	1513:1525	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	11	42	from	enriched	1442:1449	arg1	composition					1476:1486	DD-derived microbiota composition	1454:1486	DD-derived microbiota composition	1454:1486	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	11	42	from	enriched	1442:1449	arg1	Biffdobacterium					1493:1507	Biffdobacterium	1493:1507	Biffdobacterium	1493:1507	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	4	43	theme	forced	528:533	arg1	test					544:547	forced swimming test	528:547	forced swimming test	528:547	The behaviors were studied by forced swimming test, open field test, sucrose preference test, and body weight.
36550591	15	44	from	behaviors	2076:2084	arg1	rats					2154:2157	depression rats	2143:2157	depression rats	2143:2157	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	15	45	theme	animal	2069:2074	arg1	behaviors					2076:2084	animal behaviors	2069:2084	animal behaviors	2069:2084	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	14	46	theme	2-DG	1773:1776	arg1	uptake					1778:1783	2-DG uptake	1773:1783	2-DG uptake	1773:1783	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	15	47	theme	neurotransmitter	2113:2128	arg1	synthesis					2130:2138	neurotransmitter synthesis	2113:2138	neurotransmitter synthesis in depression rats	2113:2157	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	5	48	theme	hypothalamus-pituitary-adrenocortical	621:657	arg1	axis					665:668	hypothalamus-pituitary-adrenocortical (HPA) axis	621:668	hypothalamus-pituitary-adrenocortical (HPA) axis	621:668	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	5	48	theme	hypothalamus-pituitary-adrenocortical	621:657	arg1	HPA					660:662	HPA	660:662	HPA	660:662	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	9	49	theme	western	1156:1162	arg1	blotting					1164:1171	western blotting	1156:1171	western blotting	1156:1171	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	15	50	from	dysfunction	2096:2106	arg1	rats					2154:2157	depression rats	2143:2157	depression rats	2143:2157	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	10	51	theme	XPJYF	1375:1379	arg1	8 mg/ml					1364:1370	8 mg/ml	1364:1370	8 mg/ml of XPJYF (H12-8) for 12 h	1364:1396	RESULTS Microbiota composition during 8 mg/ml of XPJYF (H12-8) for 12 h showed the more consistency.
36550591	4	52	theme	sucrose	567:573	arg1	test					586:589	sucrose preference test	567:589	sucrose preference test	567:589	The behaviors were studied by forced swimming test, open field test, sucrose preference test, and body weight.
36550591	10	53	dep	RESULTS	1326:1332	arg1	showed					1398:1403	showed	1398:1403	showed the more consistency	1398:1424	RESULTS Microbiota composition during 8 mg/ml of XPJYF (H12-8) for 12 h showed the more consistency.
36550591	3	54	with	rats	410:413	arg1	administration					437:450	the intragastric administration	420:450	the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day	420:495	Some of microbiota were transplanted into germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day.
36550591	15	55	from	synthesis	2130:2138	arg1	rats					2154:2157	depression rats	2143:2157	depression rats	2143:2157	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	3	56	from	dose	474:477	arg1	administration					437:450	the intragastric administration	420:450	the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day	420:495	Some of microbiota were transplanted into germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day.
36550591	13	57	theme	astrocyte	1717:1725	arg1	activation					1727:1736	astrocyte activation	1717:1736	astrocyte activation in depression rats	1717:1755	XPJYF improved behaviors and repressed astrocyte activation in depression rats.
36550591	6	58	theme	fibrillary	771:780	arg1	protein					789:795	Glial fibrillary acidic protein	765:795	Glial fibrillary acidic protein (GFAP)	765:802	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	6	58	theme	fibrillary	771:780	arg1	biomarker					807:815	a biomarker	805:815	a biomarker of astrocyte	805:828	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	6	58	theme	fibrillary	771:780	arg1	GFAP					798:801	GFAP	798:801	GFAP	798:801	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	2	59	theme	depressive	299:308	arg1	DD					320:321	DD	320:321	DD	320:321	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	2	59	theme	depressive	299:308	arg1	disorder					310:317	depressive disorder	299:317	depressive disorder (DD)	299:322	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	14	60	theme	LPS-induced	1906:1916	arg1	astrocyte					1918:1926	LPS-induced astrocyte	1906:1926	LPS-induced astrocyte	1906:1926	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	13	61	theme	depression	1741:1750	arg1	rats					1752:1755	depression rats	1741:1755	depression rats	1741:1755	XPJYF improved behaviors and repressed astrocyte activation in depression rats.
36550591	5	62	theme	cytokines	699:707	arg1	Products					609:616	Products	609:616	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines	609:707	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	9	63	theme	2-deoxy-D-glucose	1227:1243	arg1	uptake					1252:1257	2-deoxy-D-glucose (2-DG) uptake	1227:1257	2-deoxy-D-glucose (2-DG) uptake	1227:1257	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	5	64	theme	axis	665:668	arg1	Products					609:616	Products	609:616	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines	609:707	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	9	65	theme	2-DG	1246:1249	arg1	uptake					1252:1257	2-deoxy-D-glucose (2-DG) uptake	1227:1257	2-deoxy-D-glucose (2-DG) uptake	1227:1257	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	11	66	theme	microbiota	1465:1474	arg1	composition					1476:1486	DD-derived microbiota composition	1454:1486	DD-derived microbiota composition	1454:1486	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	15	67	from	glycometabolim	1994:2007	arg1	astrocyte					2012:2020	astrocyte	2012:2020	astrocyte	2012:2020	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	5	68	theme	neurotransmitter	671:686	arg1	Products					609:616	Products	609:616	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines	609:707	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	9	69	theme	uptake	1252:1257	arg1	kits					1320:1323	2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits	1227:1323	2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits	1227:1323	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	5	70	theme	immunosorbent	744:756	arg1	assay					758:762	enzyme linked immunosorbent assay	730:762	enzyme linked immunosorbent assay	730:762	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	9	71	theme	adenosine	1260:1268	arg1	ATP					1284:1286	ATP	1284:1286	ATP	1284:1286	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	9	71	theme	adenosine	1260:1268	arg1	triphosphate					1270:1281	adenosine triphosphate	1260:1281	adenosine triphosphate (ATP)	1260:1287	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	11	72	from	Lactobacillus	1513:1525	arg1	enriched					1442:1449	enriched	1442:1449	enriched	1442:1449	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	2	73	with	patients	285:292	arg1	DD					320:321	DD	320:321	DD	320:321	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	2	73	with	patients	285:292	arg1	disorder					310:317	depressive disorder	299:317	depressive disorder (DD)	299:322	METHODS We collected fecal microbiota from patients with depressive disorder (DD) and cultured microbiota in vitro.
36550591	9	74	from	glycometabolism	1178:1192	arg1	astrocyte					1197:1205	astrocyte	1197:1205	astrocyte	1197:1205	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	8	75	theme	rat	1023:1025	arg1	astrocyte					1035:1043	activated rat primary astrocyte	1013:1043	activated rat primary astrocyte	1013:1043	We used lipopolysaccharide (LPS) to mimic activated rat primary astrocyte in vitro.
36550591	1	76	theme	Xingpijieyu	169:179	arg1	XPJYF					190:194	XPJYF	190:194	XPJYF	190:194	OBJECTIVE This investigation aims to determine the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis.
36550591	1	76	theme	Xingpijieyu	169:179	arg1	formula					181:187	Xingpijieyu formula	169:187	Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis	169:239	OBJECTIVE This investigation aims to determine the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis.
36550591	10	77	theme	more	1409:1412	arg1	consistency					1414:1424	the more consistency	1405:1424	the more consistency	1405:1424	RESULTS Microbiota composition during 8 mg/ml of XPJYF (H12-8) for 12 h showed the more consistency.
36550591	9	78	theme	neurotrophic	1069:1080	arg1	BDNF					1090:1093	BDNF	1090:1093	BDNF	1090:1093	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	9	78	theme	neurotrophic	1069:1080	arg1	factor					1082:1087	Brain-derived neurotrophic factor	1055:1087	Brain-derived neurotrophic factor (BDNF)	1055:1094	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	5	79	theme	enzyme	730:735	arg1	assay					758:762	enzyme linked immunosorbent assay	730:762	enzyme linked immunosorbent assay	730:762	Products of hypothalamus-pituitary-adrenocortical (HPA) axis, neurotransmitter, and serum cytokines were investigated by enzyme linked immunosorbent assay.
36550591	7	80	theme	sequencing	948:957	arg1	technology					959:968	RNA sequencing technology	944:968	RNA sequencing technology	944:968	Microbiota culturing in vitro after XPJYF treatment was analyze by 16 s RNA sequencing technology.
36550591	11	81	from	Biffdobacterium	1493:1507	arg1	enriched					1442:1449	enriched	1442:1449	enriched	1442:1449	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	12	82	theme	pathways	1668:1675	arg1	top					1649:1651	the top two	1645:1655	the top two	1645:1655	GLUCOSE1PMETAB-PWY and PWY-7328 of which biofunctions were dominantly encoded by Biffdobacterium were the top two of altered pathways.
36550591	12	82	theme	pathways	1668:1675	arg1	GLUCOSE1PMETAB-PWY					1543:1560	GLUCOSE1PMETAB-PWY	1543:1560	GLUCOSE1PMETAB-PWY	1543:1560	GLUCOSE1PMETAB-PWY and PWY-7328 of which biofunctions were dominantly encoded by Biffdobacterium were the top two of altered pathways.
36550591	12	82	theme	pathways	1668:1675	arg1	PWY-7328					1566:1573	PWY-7328	1566:1573	PWY-7328	1566:1573	GLUCOSE1PMETAB-PWY and PWY-7328 of which biofunctions were dominantly encoded by Biffdobacterium were the top two of altered pathways.
36550591	11	83	dep	Biffdobacterium	1493:1507	arg1	group					1536:1540	H12-8 group	1530:1540	H12-8 group	1530:1540	Lactococcus is enriched in DD-derived microbiota composition, and Biffdobacterium and Lactobacillus in H12-8 group.
36550591	0	84	theme	formula	30:36	arg1	Antidepression					0:13	Antidepression	0:13	Antidepression of Xingpijieyu formula	0:36	Antidepression of Xingpijieyu formula targets gut microbiota derived from depressive disorder.
36550591	14	85	theme	neurotrophic	1830:1841	arg1	BDNF					1851:1854	BDNF	1851:1854	BDNF	1851:1854	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	14	85	theme	neurotrophic	1830:1841	arg1	factor					1843:1848	brain-derived neurotrophic factor	1816:1848	brain-derived neurotrophic factor (BDNF)	1816:1855	XPJYF elevated 2-DG uptake, ATP, glucose-1-phosphate, and brain-derived neurotrophic factor (BDNF), and inhibited cytokines and oxidative stress in LPS-induced astrocyte.
36550591	4	86	theme	field	555:559	arg1	test					561:564	open field test	550:564	open field test	550:564	The behaviors were studied by forced swimming test, open field test, sucrose preference test, and body weight.
36550591	15	87	theme	HPA	2087:2089	arg1	dysfunction					2096:2106	HPA axis dysfunction	2087:2106	HPA axis dysfunction	2087:2106	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	9	88	theme	cytokines	1097:1105	arg1	factors					1129:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors	1055:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors	1055:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	0	89	theme	gut	46:48	arg1	microbiota					50:59	gut microbiota	46:59	gut microbiota derived from depressive disorder	46:92	Antidepression of Xingpijieyu formula targets gut microbiota derived from depressive disorder.
36550591	1	90	theme	-brain	229:234	arg1	axis					236:239	gut microbiota (GM)-brain axis	210:239	gut microbiota (GM)-brain axis	210:239	OBJECTIVE This investigation aims to determine the antidepressant role of Xingpijieyu formula (XPJYF) mediated via gut microbiota (GM)-brain axis.
36550591	6	91	theme	Glial	765:769	arg1	protein					789:795	Glial fibrillary acidic protein	765:795	Glial fibrillary acidic protein (GFAP)	765:802	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	6	91	theme	Glial	765:769	arg1	biomarker					807:815	a biomarker	805:815	a biomarker of astrocyte	805:828	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	6	91	theme	Glial	765:769	arg1	GFAP					798:801	GFAP	798:801	GFAP	798:801	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	3	92	theme	XPJYF	455:459	arg1	grain					461:465	XPJYF grain	455:465	XPJYF grain	455:465	Some of microbiota were transplanted into germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day.
36550591	15	93	theme	depression	2143:2152	arg1	rats					2154:2157	depression rats	2143:2157	depression rats	2143:2157	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	4	94	theme	swimming	535:542	arg1	test					544:547	forced swimming test	528:547	forced swimming test	528:547	The behaviors were studied by forced swimming test, open field test, sucrose preference test, and body weight.
36550591	15	95	theme	gut	2026:2028	arg1	modulation					2041:2050	gut microbiota modulation	2026:2050	gut microbiota modulation	2026:2050	CONCLUSION XPJYF treatment targets inflammation, activation, and glycometabolim in astrocyte via gut microbiota modulation, thereby improve animal behaviors, HPA axis dysfunction, and neurotransmitter synthesis in depression rats.
36550591	9	96	theme	stress	1122:1127	arg1	factors					1129:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors	1055:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors	1055:1135	Brain-derived neurotrophic factor (BDNF), cytokines, and oxidative stress factors were determined by western blotting, and glycometabolism in astrocyte was investigated by 2-deoxy-D-glucose (2-DG) uptake, adenosine triphosphate (ATP), and glucose-1-phosphate (G1P) kits.
36550591	3	97	theme	1.533 g/kg/day	482:495	arg1	dose					474:477	the dose	470:477	the dose of 1.533 g/kg/day	470:495	Some of microbiota were transplanted into germ-free rats with the intragastric administration of XPJYF grain at the dose of 1.533 g/kg/day.
36550591	7	98	theme	XPJYF	908:912	arg1	treatment					914:922	XPJYF treatment	908:922	XPJYF treatment	908:922	Microbiota culturing in vitro after XPJYF treatment was analyze by 16 s RNA sequencing technology.
36550591	6	99	theme	astrocyte	820:828	arg1	protein					789:795	Glial fibrillary acidic protein	765:795	Glial fibrillary acidic protein (GFAP)	765:802	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	6	99	theme	astrocyte	820:828	arg1	biomarker					807:815	a biomarker	805:815	a biomarker of astrocyte	805:828	Glial fibrillary acidic protein (GFAP), a biomarker of astrocyte, was quantified using immunofluorescence.
36550591	4	100	theme	body	596:599	arg1	weight					601:606	body weight	596:606	body weight	596:606	The behaviors were studied by forced swimming test, open field test, sucrose preference test, and body weight.
36550591	10	101	theme	Microbiota	1334:1343	arg1	composition					1345:1355	Microbiota composition	1334:1355	Microbiota composition during 8 mg/ml of XPJYF (H12-8) for 12 h	1334:1396	RESULTS Microbiota composition during 8 mg/ml of XPJYF (H12-8) for 12 h showed the more consistency.
35636334	8	0	theme	cross-linking	1468:1480	arg1	degree					1458:1463	a greater degree	1448:1463	a greater degree of cross-linking to lignin	1448:1490	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	8	1	theme	cell	1326:1329	arg1	acid					1344:1347	the total cell wall ferulic acid	1316:1347	the total cell wall ferulic acid (FA) content	1316:1360	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	8	1	theme	cell	1326:1329	arg1	FA					1350:1351	FA	1350:1351	FA	1350:1351	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	8	2	link	ether-linked	1411:1422	arg1	FA					1424:1425	ether-linked FA	1411:1425	ether-linked FA	1411:1425	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	9	3	theme	wall	1523:1526	arg1	reinforcement					1497:1509	The reinforcement	1493:1509	The reinforcement	1493:1509	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	9	3	theme	wall	1523:1526	arg1	strategy					1665:1672	a strategy to protect the photosystems	1663:1700	a strategy to protect the photosystems	1663:1700	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	6	4	theme	cellulose	1081:1089	arg1	level					1091:1095	a reduced cellulose level	1071:1095	a reduced cellulose level in F2	1071:1101	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	7	5	from	composition	1136:1146	arg1	mutant					1157:1162	F2bm3 mutant	1151:1162	F2bm3 mutant	1151:1162	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	2	6	theme	O-methyltransferase	475:493	arg1	gene					502:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	7	theme	caffeic	462:468	arg1	gene					502:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	1	8	theme	cereal	177:182	arg1	crops					184:188	the major cereal crops	167:188	the major cereal crops in the world	167:201	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	0	9	from	effects	60:66	arg1	photosynthetic					71:84	photosynthetic	71:84	photosynthetic	71:84	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	0	9	from	effects	60:66	arg1	response					115:122	response	115:122	response to chilling	115:134	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	0	9	from	effects	60:66	arg1	metabolisms					100:110	cell wall metabolisms	90:110	cell wall metabolisms	90:110	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	2	10	theme	COMT	496:499	arg1	gene					502:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	9	11	theme	higher	1566:1571	arg1	concentration					1573:1585	a higher concentration	1564:1585	a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling	1564:1651	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	8	12	theme	ferulic	1336:1342	arg1	acid					1344:1347	the total cell wall ferulic acid	1316:1347	the total cell wall ferulic acid (FA) content	1316:1360	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	8	12	theme	ferulic	1336:1342	arg1	FA					1350:1351	FA	1350:1351	FA	1350:1351	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	7	13	theme	higher	1254:1259	arg1	proportion					1261:1270	a higher proportion	1252:1270	a higher proportion of syringyl (S) residues in F2	1252:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	7	14	with	lignin	1240:1245	arg1	proportion					1261:1270	a higher proportion	1252:1270	a higher proportion of syringyl (S) residues in F2	1252:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	5	15	theme	sucrose	869:875	arg1	concentrations					851:864	concentrations	851:864	concentrations of sucrose, fructose and starch	851:896	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	9	16	from	concentration	1573:1585	arg1	FA					1606:1607	cell-wall-bound FA	1590:1607	cell-wall-bound FA observed in F2bm3 as a response to chilling	1590:1651	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	2	17	from	mutation	446:453	arg1	gene					502:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	3	18	theme	plant	534:538	arg1	biomass					540:546	the plant biomass	530:546	the plant biomass	530:546	Results revealed that the plant biomass was reduced, and this was more pronounced in F2bm3.
35636334	1	19	theme	mays	148:151	arg1	L.					153:154	Zea mays L.	144:154	Zea mays L.	144:154	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	1	19	theme	mays	148:151	arg1	Maize					137:141	Maize	137:141	Maize (Zea mays L.)	137:155	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	9	20	from	H-units	1552:1558	arg1	FA					1606:1607	cell-wall-bound FA	1590:1607	cell-wall-bound FA observed in F2bm3 as a response to chilling	1590:1651	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	4	21	theme	pigment	681:687	arg1	content					689:695	photosynthetic pigment content	666:695	photosynthetic pigment content between F2bm3 and F2	666:716	Photosynthesis was altered in both lines with distinct changes in photosynthetic pigment content between F2bm3 and F2, indicating an alternative photoprotection mechanism between lines under chilling.
35636334	0	22	theme	cell	90:93	arg1	metabolisms					100:110	cell wall metabolisms	90:110	cell wall metabolisms	90:110	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	5	23	located	observed	827:834	arg2	remobilization					808:821	Starch remobilization	801:821	Starch remobilization	801:821	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	5	23	located	observed	827:834	arg1	F2bm3					839:843	F2bm3	839:843	F2bm3	839:843	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	6	24	from	level	1091:1095	arg1	composition					1031:1041	the composition	1027:1041	the composition of glucuronorabinoxylan	1027:1065	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	6	24	from	level	1091:1095	arg1	F2					1100:1101	F2	1100:1101	F2	1100:1101	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	2	25	theme	near-isogenic	390:402	arg1	F2bm3					426:430	F2bm3	426:430	F2bm3	426:430	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	25	theme	near-isogenic	390:402	arg1	mutant					418:423	a low-lignin near-isogenic brown midrib3 mutant	377:423	a low-lignin near-isogenic brown midrib3 mutant (F2bm3)	377:431	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	4	26	theme	distinct	646:653	arg1	changes					655:661	distinct changes	646:661	distinct changes in photosynthetic pigment content between F2bm3 and F2	646:716	Photosynthesis was altered in both lines with distinct changes in photosynthetic pigment content between F2bm3 and F2, indicating an alternative photoprotection mechanism between lines under chilling.
35636334	2	27	theme	long	332:335	arg1	exposure					346:353	a long chilling exposure	330:353	a long chilling exposure	330:353	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	5	28	theme	fructose	878:885	arg1	concentrations					851:864	concentrations	851:864	concentrations of sucrose, fructose and starch	851:896	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	0	29	theme	low-lignin	10:19	arg1	mutant					35:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	5	30	theme	starch	891:896	arg1	concentrations					851:864	concentrations	851:864	concentrations of sucrose, fructose and starch	851:896	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	2	31	theme	inbred	358:363	arg1	line					365:368	inbred line	358:368	inbred line	358:368	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	0	32	theme	midrib3	27:33	arg1	mutant					35:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	7	33	theme	units	1210:1214	arg1	proportion					1176:1185	a higher proportion	1167:1185	a higher proportion of p-hydroxyphenyl (H) units	1167:1214	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	2	34	theme	midrib3	410:416	arg1	F2bm3					426:430	F2bm3	426:430	F2bm3	426:430	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	34	theme	midrib3	410:416	arg1	mutant					418:423	a low-lignin near-isogenic brown midrib3 mutant	377:423	a low-lignin near-isogenic brown midrib3 mutant (F2bm3)	377:431	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	4	35	theme	photoprotection	745:759	arg1	mechanism					761:769	an alternative photoprotection mechanism	730:769	an alternative photoprotection mechanism between lines under chilling	730:798	Photosynthesis was altered in both lines with distinct changes in photosynthetic pigment content between F2bm3 and F2, indicating an alternative photoprotection mechanism between lines under chilling.
35636334	2	36	theme	wall	288:291	arg1	metabolisms					293:303	cell wall metabolisms	283:303	cell wall metabolisms	283:303	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	7	37	theme	H	1207:1207	arg1	units					1210:1214	p-hydroxyphenyl (H) units	1190:1214	p-hydroxyphenyl (H) units	1190:1214	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	1	38	theme	crops	184:188	arg1	crops					184:188	the major cereal crops	167:188	the major cereal crops in the world	167:201	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	1	38	theme	crops	184:188	arg1	one					160:162	one	160:162	one	160:162	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	7	39	theme	p-hydroxyphenyl	1190:1204	arg1	units					1210:1214	p-hydroxyphenyl (H) units	1190:1214	p-hydroxyphenyl (H) units	1190:1214	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	5	40	theme	sugar	936:940	arg1	partitioning					942:953	a reduced sugar partitioning	926:953	a reduced sugar partitioning	926:953	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	8	41	theme	greater	1450:1456	arg1	degree					1458:1463	a greater degree	1448:1463	a greater degree of cross-linking to lignin	1448:1490	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	3	42	from	pronounced	579:588	arg1	F2bm3					593:597	F2bm3	593:597	F2bm3	593:597	Results revealed that the plant biomass was reduced, and this was more pronounced in F2bm3.
35636334	6	43	from	changes	1016:1022	arg1	composition					1031:1041	the composition	1027:1041	the composition of glucuronorabinoxylan	1027:1065	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	6	43	from	changes	1016:1022	arg1	F2					1100:1101	F2	1100:1101	F2	1100:1101	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	8	44	from	increase	1399:1406	arg1	F2bm3					1430:1434	F2bm3	1430:1434	F2bm3	1430:1434	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	8	44	from	increase	1399:1406	arg1	FA					1424:1425	ether-linked FA	1411:1425	ether-linked FA	1411:1425	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	9	45	with	wall	1523:1526	arg1	lignin					1533:1538	lignin	1533:1538	lignin	1533:1538	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	9	46	theme	cell	1518:1521	arg1	wall					1523:1526	the cell wall	1514:1526	the cell wall with lignin	1514:1538	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	5	47	theme	Starch	801:806	arg1	remobilization					808:821	Starch remobilization	801:821	Starch remobilization	801:821	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	6	48	theme	reduced	1073:1079	arg1	level					1091:1095	a reduced cellulose level	1071:1095	a reduced cellulose level in F2	1071:1101	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	2	49	dep	line	365:368	arg1	F2					370:371	F2	370:371	F2	370:371	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	50	theme	acid	470:473	arg1	gene					502:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	the caffeic acid O-methyltransferase (COMT) gene	458:505	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	8	51	theme	total	1320:1324	arg1	acid					1344:1347	the total cell wall ferulic acid	1316:1347	the total cell wall ferulic acid (FA) content	1316:1360	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	8	51	theme	total	1320:1324	arg1	FA					1350:1351	FA	1350:1351	FA	1350:1351	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	7	52	theme	residues	1288:1295	arg1	proportion					1261:1270	a higher proportion	1252:1270	a higher proportion of syringyl (S) residues in F2	1252:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	3	53	from	F2bm3	593:597	arg1	pronounced					579:588	pronounced	579:588	pronounced	579:588	Results revealed that the plant biomass was reduced, and this was more pronounced in F2bm3.
35636334	1	54	theme	major	171:175	arg1	crops					184:188	the major cereal crops	167:188	the major cereal crops in the world	167:201	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	8	55	theme	wall	1331:1334	arg1	acid					1344:1347	the total cell wall ferulic acid	1316:1347	the total cell wall ferulic acid (FA) content	1316:1360	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	8	55	theme	wall	1331:1334	arg1	FA					1350:1351	FA	1350:1351	FA	1350:1351	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	7	56	theme	S	1285:1285	arg1	residues					1288:1295	syringyl (S) residues	1275:1295	syringyl (S) residues in F2	1275:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	7	57	theme	lignin	1121:1126	arg1	composition					1136:1146	lignin subunit composition	1121:1146	lignin subunit composition in F2bm3 mutant	1121:1162	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	8	58	theme	acid	1344:1347	arg1	content					1354:1360	the total cell wall ferulic acid (FA) content	1316:1360	the total cell wall ferulic acid (FA) content	1316:1360	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	7	59	theme	syringyl	1275:1282	arg1	residues					1288:1295	syringyl (S) residues	1275:1295	syringyl (S) residues in F2	1275:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	1	60	from	crops	184:188	arg1	world					197:201	the world	193:201	the world	193:201	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	7	61	from	residues	1288:1295	arg1	F2					1300:1301	F2	1300:1301	F2	1300:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	7	62	theme	F2bm3	1151:1155	arg1	mutant					1157:1162	F2bm3 mutant	1151:1162	F2bm3 mutant	1151:1162	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	7	63	from	F2	1300:1301	arg1	proportion					1261:1270	a higher proportion	1252:1270	a higher proportion of syringyl (S) residues in F2	1252:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	9	64	theme	cell-wall-bound	1590:1604	arg1	FA					1606:1607	cell-wall-bound FA	1590:1607	cell-wall-bound FA observed in F2bm3 as a response to chilling	1590:1651	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	7	65	theme	subunit	1128:1134	arg1	composition					1136:1146	lignin subunit composition	1121:1146	lignin subunit composition in F2bm3 mutant	1121:1162	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	1	66	theme	Zea	144:146	arg1	L.					153:154	Zea mays L.	144:154	Zea mays L.	144:154	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	1	66	theme	Zea	144:146	arg1	Maize					137:141	Maize	137:141	Maize (Zea mays L.)	137:155	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	0	67	theme	wall	95:98	arg1	metabolisms					100:110	cell wall metabolisms	90:110	cell wall metabolisms	90:110	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	2	68	from	changes	253:259	arg1	photosynthetic					264:277	photosynthetic	264:277	photosynthetic	264:277	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	68	from	changes	253:259	arg1	metabolisms					293:303	cell wall metabolisms	283:303	cell wall metabolisms	283:303	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	4	69	theme	photosynthetic	666:679	arg1	content					689:695	photosynthetic pigment content	666:695	photosynthetic pigment content between F2bm3 and F2	666:716	Photosynthesis was altered in both lines with distinct changes in photosynthetic pigment content between F2bm3 and F2, indicating an alternative photoprotection mechanism between lines under chilling.
35636334	9	70	located	observed	1609:1616	arg1	F2bm3					1621:1625	F2bm3	1621:1625	F2bm3	1621:1625	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	9	70	located	observed	1609:1616	arg2	FA					1606:1607	cell-wall-bound FA	1590:1607	cell-wall-bound FA observed in F2bm3 as a response to chilling	1590:1651	The reinforcement of the cell wall with lignin enriched in H-units and a higher concentration in cell-wall-bound FA observed in F2bm3 as a response to chilling, could be a strategy to protect the photosystems.
35636334	2	71	theme	chilling	337:344	arg1	exposure					346:353	a long chilling exposure	330:353	a long chilling exposure	330:353	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	0	72	theme	maize	4:8	arg1	mutant					35:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	4	73	from	changes	655:661	arg1	content					689:695	photosynthetic pigment content	666:695	photosynthetic pigment content between F2bm3 and F2	666:716	Photosynthesis was altered in both lines with distinct changes in photosynthetic pigment content between F2bm3 and F2, indicating an alternative photoprotection mechanism between lines under chilling.
35636334	7	74	from	proportion	1261:1270	arg1	F2					1300:1301	F2	1300:1301	F2	1300:1301	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	2	75	theme	low-lignin	379:388	arg1	F2bm3					426:430	F2bm3	426:430	F2bm3	426:430	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	75	theme	low-lignin	379:388	arg1	mutant					418:423	a low-lignin near-isogenic brown midrib3 mutant	377:423	a low-lignin near-isogenic brown midrib3 mutant (F2bm3)	377:431	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	0	76	theme	brown	21:25	arg1	mutant					35:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant	0:40	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	1	77	theme	low	230:232	arg1	temperature					234:244	low temperature	230:244	low temperature	230:244	Maize (Zea mays L.) is one of the major cereal crops in the world and is highly sensitive to low temperature.
35636334	8	78	theme	ether-linked	1411:1422	arg1	FA					1424:1425	ether-linked FA	1411:1425	ether-linked FA	1411:1425	On average, the total cell wall ferulic acid (FA) content increased in both genotypes, with an increase in ether-linked FA in F2bm3, suggesting a greater degree of cross-linking to lignin.
35636334	6	79	theme	cell	966:969	arg1	wall					971:974	The cell wall	962:974	The cell wall	962:974	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	0	80	theme	pleiotropic	48:58	arg1	effects					60:66	pleiotropic effects	48:66	pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling	48:134	The maize low-lignin brown midrib3 mutant shows pleiotropic effects on photosynthetic and cell wall metabolisms in response to chilling.
35636334	6	81	theme	glucuronorabinoxylan	1046:1065	arg1	composition					1031:1041	the composition	1027:1041	the composition of glucuronorabinoxylan	1027:1065	The cell wall was altered upon chilling, resulting in changes in the composition of glucuronorabinoxylan and a reduced cellulose level in F2.
35636334	2	82	theme	brown	404:408	arg1	F2bm3					426:430	F2bm3	426:430	F2bm3	426:430	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	82	theme	brown	404:408	arg1	mutant					418:423	a low-lignin near-isogenic brown midrib3 mutant	377:423	a low-lignin near-isogenic brown midrib3 mutant (F2bm3)	377:431	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	7	83	dep	shifted	1113:1119	arg1	whereas					1217:1223	whereas	1217:1223	whereas	1217:1223	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	4	84	theme	alternative	733:743	arg1	mechanism					761:769	an alternative photoprotection mechanism	730:769	an alternative photoprotection mechanism between lines under chilling	730:798	Photosynthesis was altered in both lines with distinct changes in photosynthetic pigment content between F2bm3 and F2, indicating an alternative photoprotection mechanism between lines under chilling.
35636334	2	85	theme	cell	283:286	arg1	metabolisms					293:303	cell wall metabolisms	283:303	cell wall metabolisms	283:303	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	86	contain	has	440:442	arg1	mutant					418:423	a low-lignin near-isogenic brown midrib3 mutant	377:423	a low-lignin near-isogenic brown midrib3 mutant (F2bm3)	377:431	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	86	contain	has	440:442	arg1	F2bm3					426:430	F2bm3	426:430	F2bm3	426:430	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	86	contain	has	440:442	arg2	mutation					446:453	a mutation	444:453	a mutation in the caffeic acid O-methyltransferase (COMT) gene	444:505	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	2	86	contain	has	440:442	arg1	line					365:368	inbred line	358:368	inbred line	358:368	Here, changes in photosynthetic and cell wall metabolisms were investigated during a long chilling exposure in inbred line F2 and a low-lignin near-isogenic brown midrib3 mutant (F2bm3), which has a mutation in the caffeic acid O-methyltransferase (COMT) gene.
35636334	5	87	theme	reduced	928:934	arg1	partitioning					942:953	a reduced sugar partitioning	926:953	a reduced sugar partitioning	926:953	Starch remobilization was observed in F2bm3 while concentrations of sucrose, fructose and starch increased in F2, suggesting a reduced sugar partitioning in F2.
35636334	7	88	theme	higher	1169:1174	arg1	proportion					1176:1185	a higher proportion	1167:1185	a higher proportion of p-hydroxyphenyl (H) units	1167:1214	Chilling shifted lignin subunit composition in F2bm3 mutant to a higher proportion of p-hydroxyphenyl (H) units, whereas it resulted in lignin with a higher proportion of syringyl (S) residues in F2.
35636334	4	89	with	lines	635:639	arg1	changes					655:661	distinct changes	646:661	distinct changes in photosynthetic pigment content between F2bm3 and F2	646:716	Photosynthesis was altered in both lines with distinct changes in photosynthetic pigment content between F2bm3 and F2, indicating an alternative photoprotection mechanism between lines under chilling.
36169283	7	0	theme	effects	1451:1457	arg1	disadvantages					1414:1426	the disadvantages	1410:1426	the disadvantages of high price and side effects	1410:1457	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	6	1	theme	cerasifera	1218:1227	arg1	application					1200:1210	the potential application	1186:1210	the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases	1186:1310	Therefore, this research highlighted the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases.
36169283	2	2	theme	immunosorbent	299:311	arg1	ELISA					320:324	ELISA	320:324	ELISA	320:324	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	2	theme	immunosorbent	299:311	arg1	assay					313:317	enzyme-linked immunosorbent assay	285:317	enzyme-linked immunosorbent assay (ELISA)	285:325	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	4	3	theme	signaling	665:673	arg1	pathway					675:681	MAPK signaling pathway	660:681	MAPK signaling pathway	660:681	The network pharmacology analysis results indicated that EHP was related to TNF, inflammatory cytokine, and MAPK signaling pathway.
36169283	8	4	from	development	1470:1480	arg1	resources					1522:1530	natural resources	1514:1530	natural resources	1514:1530	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	5	5	theme	nuclear	843:849	arg1	factors					865:871	nuclear transcription factors	843:871	nuclear transcription factors κB (p65)	843:880	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	7	6	theme	high	1431:1434	arg1	price					1436:1440	high price	1431:1440	high price	1431:1440	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	9	7	theme	anti-inflammatory	1660:1676	arg1	effect					1678:1683	the anti-inflammatory effect	1656:1683	the anti-inflammatory effect of Prunus cerasifera (EHP)	1656:1710	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	2	8	link	enzyme-linked	285:297	arg1	ELISA					320:324	ELISA	320:324	ELISA	320:324	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	8	link	enzyme-linked	285:297	arg1	assay					313:317	enzyme-linked immunosorbent assay	285:317	enzyme-linked immunosorbent assay (ELISA)	285:325	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	6	9	theme	potential	1190:1198	arg1	application					1200:1210	the potential application	1186:1210	the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases	1186:1310	Therefore, this research highlighted the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases.
36169283	10	10	theme	anti-inflammatory	1761:1777	arg1	activity					1779:1786	anti-inflammatory activity	1761:1786	anti-inflammatory activity	1761:1786	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	5	11	theme	factors	865:871	arg1	p65					877:879	p65	877:879	p65	877:879	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	11	theme	factors	865:871	arg1	synthase					800:807	inducible nitric oxide synthase	777:807	inducible nitric oxide synthase (iNOS)	777:814	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	11	theme	factors	865:871	arg1	κB					873:874	nuclear transcription factors κB	843:874	nuclear transcription factors κB (p65)	843:880	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	7	12	theme	synthetic	1359:1367	arg1	drugs					1369:1373	many synthetic drugs	1354:1373	many synthetic drugs with anti-inflammatory effect	1354:1403	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	0	13	theme	Prunus	127:132	arg1	Ehrhart					145:151	Prunus cerasifera Ehrhart	127:151	Prunus cerasifera Ehrhart	127:151	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	5	14	from	increase	743:750	arg1	factors					768:774	inflammatory factors	755:774	inflammatory factors	755:774	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	1	15	theme	Prunus	220:225	arg1	EHP					247:249	EHP	247:249	EHP	247:249	This study aimed to investigate the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP).
36169283	1	15	theme	Prunus	220:225	arg1	Ehrhart					238:244	Prunus cerasifera Ehrhart	220:244	Prunus cerasifera Ehrhart (EHP)	220:250	This study aimed to investigate the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP).
36169283	9	16	theme	cerasifera	1695:1704	arg1	effect					1678:1683	the anti-inflammatory effect	1656:1683	the anti-inflammatory effect of Prunus cerasifera (EHP)	1656:1710	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	10	17	dep	cytokines	1824:1832	arg1	activation					1889:1898	NLRP3 inflammasome activation	1870:1898	NLRP3 inflammasome activation	1870:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	17	dep	cytokines	1824:1832	arg1	cytokines					1824:1832	pro-inflammatory cytokines	1807:1832	pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation	1807:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	17	dep	cytokines	1824:1832	arg1	NF-κB					1845:1849	NF-κB	1845:1849	NF-κB	1845:1849	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	17	dep	cytokines	1824:1832	arg1	pathway					1857:1863	MAPK pathway	1852:1863	MAPK pathway	1852:1863	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	17	dep	cytokines	1824:1832	arg1	secretion					1834:1842	secretion	1834:1842	secretion	1834:1842	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	0	18	theme	Ehrhart	145:151	arg1	mechanism					114:122	the potential anti-inflammatory mechanism	82:122	the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart	82:151	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	10	19	theme	pro-inflammatory	1807:1822	arg1	activation					1889:1898	NLRP3 inflammasome activation	1870:1898	NLRP3 inflammasome activation	1870:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	19	theme	pro-inflammatory	1807:1822	arg1	cytokines					1824:1832	pro-inflammatory cytokines	1807:1832	pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation	1807:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	19	theme	pro-inflammatory	1807:1822	arg1	NF-κB					1845:1849	NF-κB	1845:1849	NF-κB	1845:1849	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	19	theme	pro-inflammatory	1807:1822	arg1	pathway					1857:1863	MAPK pathway	1852:1863	MAPK pathway	1852:1863	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	19	theme	pro-inflammatory	1807:1822	arg1	secretion					1834:1842	secretion	1834:1842	secretion	1834:1842	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	1	20	theme	Ehrhart	238:244	arg1	activity					208:215	the anti-inflammatory activity	186:215	the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP)	186:250	This study aimed to investigate the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP).
36169283	7	21	theme	recent	1340:1345	arg1	years					1347:1351	recent years	1340:1351	recent years	1340:1351	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	5	22	theme	Western	694:700	arg1	results					707:713	Western blot results	694:713	Western blot results	694:713	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	6	23	theme	foods	1269:1273	arg1	development					1236:1246	the development	1232:1246	the development of anti-inflammatory foods that prevented inflammatory diseases	1232:1310	Therefore, this research highlighted the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases.
36169283	5	24	theme	MAPK	883:886	arg1	synthase					800:807	inducible nitric oxide synthase	777:807	inducible nitric oxide synthase (iNOS)	777:814	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	24	theme	MAPK	883:886	arg1	pathway					888:894	MAPK pathway	883:894	MAPK pathway	883:894	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	25	theme	blot	702:705	arg1	results					707:713	Western blot results	694:713	Western blot results	694:713	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	2	26	theme	network	263:269	arg1	pharmacology					271:282	network pharmacology	263:282	network pharmacology	263:282	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	7	27	theme	PRACTICAL	1313:1321	arg1	APPLICATIONS					1323:1334	PRACTICAL APPLICATIONS	1313:1334	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.	1313:1458	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	5	28	theme	factor	1074:1079	arg1	pathway					1140:1146	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	8	29	contain	has	1532:1534	arg1	development					1470:1480	the development	1466:1480	the development of anti-inflammatory drugs from natural resources	1466:1530	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	8	29	contain	has	1532:1534	arg2	value					1552:1556	its application value	1536:1556	its application value	1536:1556	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	5	30	theme	inducible	777:785	arg1	synthase					800:807	inducible nitric oxide synthase	777:807	inducible nitric oxide synthase (iNOS)	777:814	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	30	theme	inducible	777:785	arg1	pathway					888:894	MAPK pathway	883:894	MAPK pathway	883:894	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	30	theme	inducible	777:785	arg1	κB					873:874	nuclear transcription factors κB	843:874	nuclear transcription factors κB (p65)	843:880	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	30	theme	inducible	777:785	arg1	iNOS					810:813	iNOS	810:813	iNOS	810:813	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	30	theme	inducible	777:785	arg1	cyclooxygenase					817:830	cyclooxygenase 2	817:832	cyclooxygenase 2 (COX-2)	817:840	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	30	theme	inducible	777:785	arg1	proteins					916:923	pyrolytic relevant proteins	897:923	pyrolytic relevant proteins	897:923	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	31	theme	2-related	1091:1099	arg1	HO-1					1134:1137	HO-1	1134:1137	HO-1	1134:1137	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	31	theme	2-related	1091:1099	arg1	/hemeoxygenase-1					1116:1131	2-related factor 2 (Nrf2)/hemeoxygenase-1	1091:1131	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	11	32	contain	has	1926:1928	arg1	cerasifera					1915:1924	P. cerasifera	1912:1924	P. cerasifera	1912:1924	Therefore, P. cerasifera has the potential to develop into functional food to prevent the progress of various inflammatory-related diseases.
36169283	11	32	contain	has	1926:1928	arg2	potential					1934:1942	the potential to develop into functional food to prevent the progress of various inflammatory-related diseases	1930:2039	the potential to develop into functional food to prevent the progress of various inflammatory-related diseases	1930:2039	Therefore, P. cerasifera has the potential to develop into functional food to prevent the progress of various inflammatory-related diseases.
36169283	8	33	from	resources	1522:1530	arg1	development					1470:1480	the development	1466:1480	the development of anti-inflammatory drugs from natural resources	1466:1530	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	8	33	from	resources	1522:1530	arg1	drugs					1503:1507	anti-inflammatory drugs	1485:1507	anti-inflammatory drugs from natural resources	1485:1530	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	0	34	theme	potential	86:94	arg1	mechanism					114:122	the potential anti-inflammatory mechanism	82:122	the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart	82:151	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	11	35	theme	diseases	2032:2039	arg1	progress					1991:1998	the progress	1987:1998	the progress of various inflammatory-related diseases	1987:2039	Therefore, P. cerasifera has the potential to develop into functional food to prevent the progress of various inflammatory-related diseases.
36169283	5	36	theme	family	943:948	arg1	NLRP3					977:981	NLRP3	977:981	NLRP3	977:981	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	36	theme	family	943:948	arg1	pyrin					950:954	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3	777:974	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3)	777:982	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	8	37	theme	anti-inflammatory	1485:1501	arg1	drugs					1503:1507	anti-inflammatory drugs	1485:1507	anti-inflammatory drugs from natural resources	1485:1530	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	5	38	theme	domain-containing	956:972	arg1	NLRP3					977:981	NLRP3	977:981	NLRP3	977:981	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	38	theme	domain-containing	956:972	arg1	pyrin					950:954	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3	777:974	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3)	777:982	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	7	39	with	drugs	1369:1373	arg1	effect					1398:1403	anti-inflammatory effect	1380:1403	anti-inflammatory effect	1380:1403	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	5	40	theme	/hemeoxygenase-1	1116:1131	arg1	pathway					1140:1146	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	41	theme	relevant	907:914	arg1	proteins					916:923	pyrolytic relevant proteins	897:923	pyrolytic relevant proteins	897:923	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	41	theme	relevant	907:914	arg1	synthase					800:807	inducible nitric oxide synthase	777:807	inducible nitric oxide synthase (iNOS)	777:814	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	2	42	theme	EHP	451:453	arg1	composition					400:410	the chemical composition	387:410	the chemical composition	387:410	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	42	theme	EHP	451:453	arg1	mechanism					438:446	the anti-inflammatory mechanism	416:446	the anti-inflammatory mechanism of EHP	416:453	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	4	43	theme	network	556:562	arg1	results					586:592	The network pharmacology analysis results	552:592	The network pharmacology analysis results	552:592	The network pharmacology analysis results indicated that EHP was related to TNF, inflammatory cytokine, and MAPK signaling pathway.
36169283	0	44	theme	pharmacology	10:21	arg1	strategy					23:30	A network pharmacology strategy	0:30	A network pharmacology strategy	0:30	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	5	45	theme	nod-like	925:932	arg1	NLRP3					977:981	NLRP3	977:981	NLRP3	977:981	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	45	theme	nod-like	925:932	arg1	pyrin					950:954	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3	777:974	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3)	777:982	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	4	46	theme	analysis	577:584	arg1	results					586:592	The network pharmacology analysis results	552:592	The network pharmacology analysis results	552:592	The network pharmacology analysis results indicated that EHP was related to TNF, inflammatory cytokine, and MAPK signaling pathway.
36169283	3	47	theme	LC-MS/MS	460:467	arg1	results					469:475	The LC-MS/MS results	456:475	The LC-MS/MS results	456:475	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	5	48	theme	oxide	794:798	arg1	synthase					800:807	inducible nitric oxide synthase	777:807	inducible nitric oxide synthase (iNOS)	777:814	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	48	theme	oxide	794:798	arg1	pathway					888:894	MAPK pathway	883:894	MAPK pathway	883:894	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	48	theme	oxide	794:798	arg1	κB					873:874	nuclear transcription factors κB	843:874	nuclear transcription factors κB (p65)	843:880	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	48	theme	oxide	794:798	arg1	iNOS					810:813	iNOS	810:813	iNOS	810:813	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	48	theme	oxide	794:798	arg1	cyclooxygenase					817:830	cyclooxygenase 2	817:832	cyclooxygenase 2 (COX-2)	817:840	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	48	theme	oxide	794:798	arg1	proteins					916:923	pyrolytic relevant proteins	897:923	pyrolytic relevant proteins	897:923	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	7	49	contain	have	1405:1408	arg2	disadvantages					1414:1426	the disadvantages	1410:1426	the disadvantages of high price and side effects	1410:1457	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	7	49	contain	have	1405:1408	arg1	drugs					1369:1373	many synthetic drugs	1354:1373	many synthetic drugs with anti-inflammatory effect	1354:1403	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	0	50	dep	in	46:47	arg1	vitro					49:53	vitro	49:53	vitro	49:53	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	10	51	theme	MAPK	1852:1855	arg1	cytokines					1824:1832	pro-inflammatory cytokines	1807:1832	pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation	1807:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	51	theme	MAPK	1852:1855	arg1	pathway					1857:1863	MAPK pathway	1852:1863	MAPK pathway	1852:1863	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	3	52	theme	phenolic	504:511	arg1	flavonoids					489:498	flavonoids	489:498	flavonoids	489:498	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	3	52	theme	phenolic	504:511	arg1	acids					513:517	phenolic acids	504:517	phenolic acids	504:517	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	3	52	theme	phenolic	504:511	arg1	compounds					534:542	the major compounds	524:542	the major compounds in EHP	524:549	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	0	53	theme	in	46:47	arg1	experiments					55:65	in vitro experiments	46:65	in vitro experiments	46:65	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	5	54	theme	Nrf2	1111:1114	arg1	HO-1					1134:1137	HO-1	1134:1137	HO-1	1134:1137	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	54	theme	Nrf2	1111:1114	arg1	/hemeoxygenase-1					1116:1131	2-related factor 2 (Nrf2)/hemeoxygenase-1	1091:1131	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	9	55	theme	RAW264.7	1589:1596	arg1	cells					1598:1602	LPS-stimulated RAW264.7 cells	1574:1602	LPS-stimulated RAW264.7 cells	1574:1602	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	2	56	theme	Western	332:338	arg1	analysis					345:352	Western blot analysis	332:352	Western blot analysis	332:352	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	11	57	theme	various	2003:2009	arg1	diseases					2032:2039	various inflammatory-related diseases	2003:2039	various inflammatory-related diseases	2003:2039	Therefore, P. cerasifera has the potential to develop into functional food to prevent the progress of various inflammatory-related diseases.
36169283	10	58	theme	NLRP3	1870:1874	arg1	cytokines					1824:1832	pro-inflammatory cytokines	1807:1832	pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation	1807:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	58	theme	NLRP3	1870:1874	arg1	activation					1889:1898	NLRP3 inflammasome activation	1870:1898	NLRP3 inflammasome activation	1870:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	3	59	theme	major	528:532	arg1	flavonoids					489:498	flavonoids	489:498	flavonoids	489:498	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	3	59	theme	major	528:532	arg1	acids					513:517	phenolic acids	504:517	phenolic acids	504:517	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	3	59	theme	major	528:532	arg1	compounds					534:542	the major compounds	524:542	the major compounds in EHP	524:549	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	2	60	dep	LC-MS/MS	253:260	arg1	methods					354:360	methods	354:360	methods	354:360	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	6	61	from	application	1200:1210	arg1	development					1236:1246	the development	1232:1246	the development of anti-inflammatory foods that prevented inflammatory diseases	1232:1310	Therefore, this research highlighted the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases.
36169283	7	62	theme	side	1446:1449	arg1	effects					1451:1457	side effects	1446:1457	side effects	1446:1457	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	9	63	theme	inflammatory	1627:1638	arg1	model					1640:1644	inflammatory model	1627:1644	inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP)	1627:1710	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	11	64	theme	inflammatory-related	2011:2030	arg1	diseases					2032:2039	various inflammatory-related diseases	2003:2039	various inflammatory-related diseases	2003:2039	Therefore, P. cerasifera has the potential to develop into functional food to prevent the progress of various inflammatory-related diseases.
36169283	7	65	theme	price	1436:1440	arg1	disadvantages					1414:1426	the disadvantages	1410:1426	the disadvantages of high price and side effects	1410:1457	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	3	66	from	compounds	534:542	arg1	EHP					547:549	EHP	547:549	EHP	547:549	The LC-MS/MS results showed that flavonoids and phenolic acids were the major compounds in EHP.
36169283	2	67	theme	enzyme-linked	285:297	arg1	ELISA					320:324	ELISA	320:324	ELISA	320:324	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	67	theme	enzyme-linked	285:297	arg1	assay					313:317	enzyme-linked immunosorbent assay	285:317	enzyme-linked immunosorbent assay (ELISA)	285:325	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	1	68	theme	anti-inflammatory	190:206	arg1	activity					208:215	the anti-inflammatory activity	186:215	the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP)	186:250	This study aimed to investigate the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP).
36169283	4	69	theme	MAPK	660:663	arg1	pathway					675:681	MAPK signaling pathway	660:681	MAPK signaling pathway	660:681	The network pharmacology analysis results indicated that EHP was related to TNF, inflammatory cytokine, and MAPK signaling pathway.
36169283	5	70	theme	transcription	851:863	arg1	factors					865:871	nuclear transcription factors	843:871	nuclear transcription factors κB (p65)	843:880	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	7	71	theme	anti-inflammatory	1380:1396	arg1	effect					1398:1403	anti-inflammatory effect	1380:1403	anti-inflammatory effect	1380:1403	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	0	72	theme	cerasifera	134:143	arg1	Ehrhart					145:151	Prunus cerasifera Ehrhart	127:151	Prunus cerasifera Ehrhart	127:151	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	9	73	theme	Prunus	1688:1693	arg1	cerasifera					1695:1704	Prunus cerasifera	1688:1704	Prunus cerasifera (EHP)	1688:1710	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	9	73	theme	Prunus	1688:1693	arg1	EHP					1707:1709	EHP	1707:1709	EHP	1707:1709	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	4	74	theme	inflammatory	633:644	arg1	cytokine					646:653	inflammatory cytokine	633:653	inflammatory cytokine	633:653	The network pharmacology analysis results indicated that EHP was related to TNF, inflammatory cytokine, and MAPK signaling pathway.
36169283	6	75	theme	inflammatory	1290:1301	arg1	diseases					1303:1310	inflammatory diseases	1290:1310	inflammatory diseases	1290:1310	Therefore, this research highlighted the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases.
36169283	11	76	theme	functional	1960:1969	arg1	food					1971:1974	functional food	1960:1974	functional food	1960:1974	Therefore, P. cerasifera has the potential to develop into functional food to prevent the progress of various inflammatory-related diseases.
36169283	1	77	theme	cerasifera	227:236	arg1	EHP					247:249	EHP	247:249	EHP	247:249	This study aimed to investigate the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP).
36169283	1	77	theme	cerasifera	227:236	arg1	Ehrhart					238:244	Prunus cerasifera Ehrhart	220:244	Prunus cerasifera Ehrhart (EHP)	220:250	This study aimed to investigate the anti-inflammatory activity of Prunus cerasifera Ehrhart (EHP).
36169283	7	78	theme	many	1354:1357	arg1	drugs					1369:1373	many synthetic drugs	1354:1373	many synthetic drugs with anti-inflammatory effect	1354:1403	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	6	79	theme	anti-inflammatory	1251:1267	arg1	foods					1269:1273	anti-inflammatory foods	1251:1273	anti-inflammatory foods that prevented inflammatory diseases	1251:1310	Therefore, this research highlighted the potential application of P. cerasifera in the development of anti-inflammatory foods that prevented inflammatory diseases.
36169283	5	80	theme	inflammatory	755:766	arg1	factors					768:774	inflammatory factors	755:774	inflammatory factors	755:774	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	81	dep	EHP	727:729	arg1	impeded					731:737	impeded	731:737	impeded	731:737	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	82	theme	erythroid	1081:1089	arg1	pathway					1140:1146	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	0	83	theme	anti-inflammatory	96:112	arg1	mechanism					114:122	the potential anti-inflammatory mechanism	82:122	the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart	82:151	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	5	84	theme	factor	1101:1106	arg1	HO-1					1134:1137	HO-1	1134:1137	HO-1	1134:1137	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	84	theme	factor	1101:1106	arg1	/hemeoxygenase-1					1116:1131	2-related factor 2 (Nrf2)/hemeoxygenase-1	1091:1131	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	85	theme	receptor	934:941	arg1	NLRP3					977:981	NLRP3	977:981	NLRP3	977:981	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	85	theme	receptor	934:941	arg1	pyrin					950:954	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3	777:974	inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3)	777:982	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	10	86	contain	possessed	1751:1759	arg2	activity					1779:1786	anti-inflammatory activity	1761:1786	anti-inflammatory activity	1761:1786	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	86	contain	possessed	1751:1759	arg1	cerasifera					1740:1749	P. cerasifera	1737:1749	P. cerasifera	1737:1749	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	8	87	theme	drugs	1503:1507	arg1	development					1470:1480	the development	1466:1480	the development of anti-inflammatory drugs from natural resources	1466:1530	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	2	88	used	used	367:370	arg2	pharmacology					271:282	network pharmacology	263:282	network pharmacology	263:282	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	88	used	used	367:370	arg2	assay					313:317	enzyme-linked immunosorbent assay	285:317	enzyme-linked immunosorbent assay (ELISA)	285:325	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	88	used	used	367:370	arg2	ELISA					320:324	ELISA	320:324	ELISA	320:324	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	88	used	used	367:370	arg2	analysis					345:352	Western blot analysis	332:352	Western blot analysis	332:352	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	2	88	used	used	367:370	arg2	LC-MS/MS					253:260	LC-MS/MS	253:260	LC-MS/MS	253:260	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	7	89	dep	APPLICATIONS	1323:1334	arg1	have					1405:1408	have	1405:1408	have the disadvantages of high price and side effects	1405:1457	PRACTICAL APPLICATIONS: In recent years, many synthetic drugs with anti-inflammatory effect have the disadvantages of high price and side effects.
36169283	8	90	theme	natural	1514:1520	arg1	resources					1522:1530	natural resources	1514:1530	natural resources	1514:1530	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	2	91	theme	chemical	391:398	arg1	composition					400:410	the chemical composition	387:410	the chemical composition	387:410	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	0	92	theme	network	2:8	arg1	strategy					23:30	A network pharmacology strategy	0:30	A network pharmacology strategy	0:30	A network pharmacology strategy combined with in vitro experiments to investigate the potential anti-inflammatory mechanism of Prunus cerasifera Ehrhart.
36169283	5	93	theme	nuclear	1066:1072	arg1	factor					1074:1079	the nuclear factor	1062:1079	the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway	1062:1146	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	94	theme	pyrolytic	897:905	arg1	proteins					916:923	pyrolytic relevant proteins	897:923	pyrolytic relevant proteins	897:923	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	94	theme	pyrolytic	897:905	arg1	synthase					800:807	inducible nitric oxide synthase	777:807	inducible nitric oxide synthase (iNOS)	777:814	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	4	95	theme	pharmacology	564:575	arg1	results					586:592	The network pharmacology analysis results	552:592	The network pharmacology analysis results	552:592	The network pharmacology analysis results indicated that EHP was related to TNF, inflammatory cytokine, and MAPK signaling pathway.
36169283	8	96	theme	application	1540:1550	arg1	value					1552:1556	its application value	1536:1556	its application value	1536:1556	Thus, the development of anti-inflammatory drugs from natural resources has its application value.
36169283	2	97	theme	anti-inflammatory	420:436	arg1	mechanism					438:446	the anti-inflammatory mechanism	416:446	the anti-inflammatory mechanism of EHP	416:453	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	5	98	theme	nitric	787:792	arg1	synthase					800:807	inducible nitric oxide synthase	777:807	inducible nitric oxide synthase (iNOS)	777:814	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	98	theme	nitric	787:792	arg1	pathway					888:894	MAPK pathway	883:894	MAPK pathway	883:894	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	98	theme	nitric	787:792	arg1	κB					873:874	nuclear transcription factors κB	843:874	nuclear transcription factors κB (p65)	843:880	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	98	theme	nitric	787:792	arg1	iNOS					810:813	iNOS	810:813	iNOS	810:813	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	98	theme	nitric	787:792	arg1	cyclooxygenase					817:830	cyclooxygenase 2	817:832	cyclooxygenase 2 (COX-2)	817:840	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	5	98	theme	nitric	787:792	arg1	proteins					916:923	pyrolytic relevant proteins	897:923	pyrolytic relevant proteins	897:923	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
36169283	9	99	theme	LPS-stimulated	1574:1587	arg1	cells					1598:1602	LPS-stimulated RAW264.7 cells	1574:1602	LPS-stimulated RAW264.7 cells	1574:1602	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	9	100	used	used	1609:1612	arg2	cells					1598:1602	LPS-stimulated RAW264.7 cells	1574:1602	LPS-stimulated RAW264.7 cells	1574:1602	In this study, LPS-stimulated RAW264.7 cells were used to establish inflammatory model to verify the anti-inflammatory effect of Prunus cerasifera (EHP).
36169283	2	101	theme	blot	340:343	arg1	analysis					345:352	Western blot analysis	332:352	Western blot analysis	332:352	LC-MS/MS, network pharmacology, enzyme-linked immunosorbent assay (ELISA), and Western blot analysis methods were used to investigate the chemical composition and the anti-inflammatory mechanism of EHP.
36169283	10	102	theme	inflammasome	1876:1887	arg1	cytokines					1824:1832	pro-inflammatory cytokines	1807:1832	pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation	1807:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	10	102	theme	inflammasome	1876:1887	arg1	activation					1889:1898	NLRP3 inflammasome activation	1870:1898	NLRP3 inflammasome activation	1870:1898	The results showed that P. cerasifera possessed anti-inflammatory activity through inhibiting pro-inflammatory cytokines secretion, NF-κB, MAPK pathway, and NLRP3 inflammasome activation.
36169283	5	103	from	pyrin	950:954	arg1	factors					768:774	inflammatory factors	755:774	inflammatory factors	755:774	ELISA and Western blot results showed that EHP impeded the increase in inflammatory factors, inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX-2), nuclear transcription factors κB (p65), MAPK pathway, pyrolytic relevant proteins nod-like receptor family pyrin domain-containing 3 (NLRP3), and interleukin-1β (IL-1β) induced by lipopolysaccharide (LPS) and activated the nuclear factor erythroid 2-related factor 2 (Nrf2)/hemeoxygenase-1 (HO-1) pathway.
35687307	4	0	theme	acid	849:852	arg1	supplementation					854:868	amino acid supplementation	843:868	amino acid supplementation	843:868	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	10	1	dep	lower	1866:1870	arg1	index					1886:1890	crystallinity index	1872:1890	crystallinity index	1872:1890	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	9	2	theme	L-1	1783:1785	arg1	level					1767:1771	a production level	1754:1771	a production level of 3.02 g L-1	1754:1785	After optimization and validation steps, a production level of 3.02 g L-1 was achieved.
35687307	5	3	theme	acid	1170:1173	arg1	concentrations					1132:1145	the optimal concentrations	1120:1145	the optimal concentrations of each required amino acid	1120:1173	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	3	4	theme	nutritional	527:537	arg1	necessary					610:618	necessary	610:618	necessary	610:618	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	3	4	theme	nutritional	527:537	arg1	knowledge					510:518	the knowledge	506:518	the knowledge of the nutritional needs of microorganisms that have important industrial applications	506:605	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	1	5	contain	has	269:271	arg1	AAB					259:261	AAB	259:261	AAB	259:261	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	1	5	contain	has	269:271	arg1	bacteria					249:256	acetic acid bacteria	237:256	acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries	237:344	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	1	5	contain	has	269:271	arg2	applications					281:292	several applications	273:292	several applications	273:292	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	0	6	theme	Methodology	164:174	arg1	Application					132:142	Application	132:142	Application	132:142	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	0	6	theme	Methodology	164:174	arg1	Analysis					119:126	Effect Analysis	112:126	Effect Analysis	112:126	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	10	7	theme	crystallinity	1872:1884	arg1	index					1886:1890	crystallinity index	1872:1890	crystallinity index	1872:1890	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	8	8	theme	charged	1637:1643	arg1	acids					1651:1655	all sulfur and positively charged amino acids	1611:1655	acids	1651:1655	Conversely, all sulfur and positively charged amino acids had a negative effect and reduced the production yield.
35687307	7	9	contain	had	1468:1470	arg2	effect					1495:1500	a significant positive effect	1472:1500	a significant positive effect	1472:1500	Three amino acids had a significant positive effect and were required: aspartic acid (1.5 g L-1), phenylalanine (1.5 g L-1), and serine (3.0 g L-1).
35687307	7	9	contain	had	1468:1470	arg1	acids					1462:1466	Three amino acids	1450:1466	Three amino acids	1450:1466	Three amino acids had a significant positive effect and were required: aspartic acid (1.5 g L-1), phenylalanine (1.5 g L-1), and serine (3.0 g L-1).
35687307	10	10	theme	thermal	1904:1910	arg1	properties					1928:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	0	11	theme	Response	147:154	arg1	Methodology					164:174	Response Surface Methodology	147:174	Response Surface Methodology	147:174	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	6	12	theme	infrared	1391:1398	arg1	FT-IR					1414:1418	FT-IR	1414:1418	FT-IR	1414:1418	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	6	12	theme	infrared	1391:1398	arg1	spectroscopy					1400:1411	infrared spectroscopy	1391:1411	infrared spectroscopy (FT-IR)	1391:1419	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	4	13	theme	culture	877:883	arg1	media					885:889	the culture media	873:889	the culture media	873:889	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	6	14	theme	X-ray	1291:1295	arg1	XRD					1310:1312	XRD	1310:1312	XRD	1310:1312	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	6	14	theme	X-ray	1291:1295	arg1	diffraction					1297:1307	X-ray diffraction	1291:1307	X-ray diffraction (XRD)	1291:1313	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	3	15	theme	important	573:581	arg1	applications					594:605	important industrial applications	573:605	important industrial applications	573:605	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	3	16	dep	nutritional	527:537	arg1	needs					539:543	needs	539:543	needs	539:543	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	10	17	theme	hydrophilic	1916:1926	arg1	properties					1928:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	6	18	theme	differential	1350:1361	arg1	DSC					1385:1387	DSC	1385:1387	DSC	1385:1387	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	6	18	theme	differential	1350:1361	arg1	calorimetry					1372:1382	differential scanning calorimetry	1350:1382	differential scanning calorimetry (DSC)	1350:1388	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	8	19	theme	production	1695:1704	arg1	yield					1706:1710	the production yield	1691:1710	the production yield	1691:1710	Conversely, all sulfur and positively charged amino acids had a negative effect and reduced the production yield.
35687307	10	20	theme	standard	1964:1971	arg1	medium					1976:1981	standard HS medium	1964:1981	standard HS medium	1964:1981	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	1	21	theme	food	330:333	arg1	industries					335:344	the medical, pharmaceutical, and food industries	297:344	the medical, pharmaceutical, and food industries	297:344	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	7	22	theme	aspartic	1521:1528	arg1	acid					1530:1533	aspartic acid	1521:1533	aspartic acid (1.5 g L-1)	1521:1545	Three amino acids had a significant positive effect and were required: aspartic acid (1.5 g L-1), phenylalanine (1.5 g L-1), and serine (3.0 g L-1).
35687307	7	22	theme	aspartic	1521:1528	arg1	L-1					1542:1544	1.5 g L-1	1536:1544	1.5 g L-1	1536:1544	Three amino acids had a significant positive effect and were required: aspartic acid (1.5 g L-1), phenylalanine (1.5 g L-1), and serine (3.0 g L-1).
35687307	4	23	theme	Komagataeibacter	927:942	arg1	V-05					956:959	Komagataeibacter intermedius V-05	927:959	Komagataeibacter intermedius V-05	927:959	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	4	23	theme	Komagataeibacter	927:942	arg1	strain					919:924	an AAB strain	912:924	an AAB strain (Komagataeibacter intermedius V-05)	912:960	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	6	24	theme	thermogravimetric	1316:1332	arg1	TGA					1344:1346	TGA	1344:1346	TGA	1344:1346	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	6	24	theme	thermogravimetric	1316:1332	arg1	analysis					1334:1341	thermogravimetric analysis	1316:1341	thermogravimetric analysis (TGA)	1316:1347	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	5	25	theme	factorial	1061:1069	arg1	design					1071:1076	a Plackett and Burman factorial design	1039:1076	a Plackett and Burman factorial design	1039:1076	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	0	26	from	Acids	39:43	arg1	Production					68:77	Bacterial Cellulose Production	48:77	Bacterial Cellulose Production by Komagataeibacter intermedius	48:109	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	4	27	theme	nutrients	730:738	arg1	choice					710:715	the choice	706:715	the choice of different nutrients as nitrogen source	706:757	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	0	28	theme	Nutritional	0:10	arg1	Supplementation					12:26	Nutritional Supplementation	0:26	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.	0:175	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	0	29	dep	Komagataeibacter	82:97	arg1	intermedius					99:109	Komagataeibacter intermedius	82:109	Komagataeibacter intermedius	82:109	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	2	30	theme	nutrients	419:427	arg1	organisms					363:371	other living organisms	350:371	other living organisms	350:371	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	2	30	theme	nutrients	419:427	arg1	sources					386:392	sources	386:392	sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production	386:499	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	4	31	theme	nitrogen	743:750	arg1	source					752:757	nitrogen source	743:757	nitrogen source	743:757	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	2	32	theme	elements	406:413	arg1	organisms					363:371	other living organisms	350:371	other living organisms	350:371	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	2	32	theme	elements	406:413	arg1	sources					386:392	sources	386:392	sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production	386:499	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	7	33	theme	significant	1474:1484	arg1	effect					1495:1500	a significant positive effect	1472:1500	a significant positive effect	1472:1500	Three amino acids had a significant positive effect and were required: aspartic acid (1.5 g L-1), phenylalanine (1.5 g L-1), and serine (3.0 g L-1).
35687307	2	34	theme	metabolite	479:488	arg1	production					490:499	metabolite production	479:499	metabolite production	479:499	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	0	35	theme	Bacterial	48:56	arg1	Production					68:77	Bacterial Cellulose Production	48:77	Bacterial Cellulose Production by Komagataeibacter intermedius	48:109	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	8	36	contain	had	1657:1659	arg1	sulfur					1615:1620	all sulfur and positively charged amino acids	1611:1655	sulfur	1615:1620	Conversely, all sulfur and positively charged amino acids had a negative effect and reduced the production yield.
35687307	8	36	contain	had	1657:1659	arg2	effect					1672:1677	a negative effect	1661:1677	a negative effect	1661:1677	Conversely, all sulfur and positively charged amino acids had a negative effect and reduced the production yield.
35687307	8	36	contain	had	1657:1659	arg1	acids					1651:1655	all sulfur and positively charged amino acids	1611:1655	acids	1651:1655	Conversely, all sulfur and positively charged amino acids had a negative effect and reduced the production yield.
35687307	4	37	theme	metabolic	783:791	arg1	effects					793:799	different metabolic effects	773:799	different metabolic effects	773:799	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	7	38	theme	amino	1456:1460	arg1	acids					1462:1466	Three amino acids	1450:1466	Three amino acids	1450:1466	Three amino acids had a significant positive effect and were required: aspartic acid (1.5 g L-1), phenylalanine (1.5 g L-1), and serine (3.0 g L-1).
35687307	2	39	theme	living	356:361	arg1	organisms					363:371	other living organisms	350:371	other living organisms	350:371	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	2	39	theme	living	356:361	arg1	sources					386:392	sources	386:392	sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production	386:499	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	6	40	theme	hydrophilic	1426:1436	arg1	properties					1438:1447	hydrophilic properties	1426:1447	hydrophilic properties	1426:1447	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	1	41	theme	acid	244:247	arg1	AAB					259:261	AAB	259:261	AAB	259:261	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	1	41	theme	acid	244:247	arg1	bacteria					249:256	acetic acid bacteria	237:256	acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries	237:344	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	9	42	theme	3.02 g	1776:1781	arg1	L-1					1783:1785	3.02 g L-1	1776:1785	3.02 g L-1	1776:1785	After optimization and validation steps, a production level of 3.02 g L-1 was achieved.
35687307	5	43	theme	amino	1164:1168	arg1	acid					1170:1173	each required amino acid	1150:1173	each required amino acid	1150:1173	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	4	44	theme	amino	843:847	arg1	supplementation					854:868	amino acid supplementation	843:868	amino acid supplementation	843:868	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	3	45	contain	have	568:571	arg2	applications					594:605	important industrial applications	573:605	important industrial applications	573:605	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	3	45	contain	have	568:571	arg1	microorganisms					548:561	microorganisms	548:561	microorganisms that have important industrial applications	548:605	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	6	46	theme	chemical	1254:1261	arg1	structure					1263:1271	chemical structure	1254:1271	chemical structure	1254:1271	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	4	47	theme	supplementation	854:868	arg1	effects					832:838	the effects	828:838	the effects of amino acid supplementation in the culture media	828:889	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	10	48	theme	optimized	1825:1833	arg1	media					1835:1839	optimized media	1825:1839	optimized media	1825:1839	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	3	49	theme	microorganisms	548:561	arg1	nutritional					527:537	nutritional	527:537	nutritional	527:537	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	5	50	theme	composite	1090:1098	arg1	design					1100:1105	central composite design	1082:1105	central composite design	1082:1105	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	10	51	theme	lower	1866:1870	arg1	properties					1928:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	4	52	theme	BC	895:896	arg1	production					898:907	BC production	895:907	BC production by an AAB strain (Komagataeibacter intermedius V-05)	895:960	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	0	53	theme	Surface	156:162	arg1	Methodology					164:174	Response Surface Methodology	147:174	Response Surface Methodology	147:174	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	3	54	theme	industrial	583:592	arg1	applications					594:605	important industrial applications	573:605	important industrial applications	573:605	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	8	55	theme	amino	1645:1649	arg1	acids					1651:1655	all sulfur and positively charged amino acids	1611:1655	acids	1651:1655	Conversely, all sulfur and positively charged amino acids had a negative effect and reduced the production yield.
35687307	6	56	theme	optimal	1201:1207	arg1	conditions					1209:1218	optimal conditions	1201:1218	optimal conditions	1201:1218	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	1	57	theme	pharmaceutical	310:323	arg1	industries					335:344	the medical, pharmaceutical, and food industries	297:344	the medical, pharmaceutical, and food industries	297:344	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	10	58	theme	greater	1896:1902	arg1	properties					1928:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	lower crystallinity index but greater thermal and hydrophilic properties	1866:1937	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	5	59	theme	Plackett	1041:1048	arg1	design					1071:1076	a Plackett and Burman factorial design	1039:1076	a Plackett and Burman factorial design	1039:1076	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	8	60	theme	negative	1663:1670	arg1	effect					1672:1677	a negative effect	1661:1677	a negative effect	1661:1677	Conversely, all sulfur and positively charged amino acids had a negative effect and reduced the production yield.
35687307	6	61	theme	scanning	1363:1370	arg1	DSC					1385:1387	DSC	1385:1387	DSC	1385:1387	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	6	61	theme	scanning	1363:1370	arg1	calorimetry					1372:1382	differential scanning calorimetry	1350:1382	differential scanning calorimetry (DSC)	1350:1388	Membranes produced under optimal conditions were characterized in relation to chemical structure and properties by X-ray diffraction (XRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), infrared spectroscopy (FT-IR), and hydrophilic properties.
35687307	4	62	theme	intermedius	944:954	arg1	V-05					956:959	Komagataeibacter intermedius V-05	927:959	Komagataeibacter intermedius V-05	927:959	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	4	62	theme	intermedius	944:954	arg1	strain					919:924	an AAB strain	912:924	an AAB strain (Komagataeibacter intermedius V-05)	912:960	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	5	63	theme	central	1082:1088	arg1	design					1100:1105	central composite design	1082:1105	central composite design	1082:1105	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	1	64	theme	Bacterial	177:185	arg1	biopolymer					207:216	a biopolymer	205:216	a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries	205:344	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	1	64	theme	Bacterial	177:185	arg1	BC					198:199	BC	198:199	BC	198:199	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	1	64	theme	Bacterial	177:185	arg1	cellulose					187:195	Bacterial cellulose	177:195	Bacterial cellulose (BC)	177:200	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	0	65	theme	Effect	112:117	arg1	Analysis					119:126	Effect Analysis	112:126	Effect Analysis	112:126	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	4	66	theme	AAB	915:917	arg1	V-05					956:959	Komagataeibacter intermedius V-05	927:959	Komagataeibacter intermedius V-05	927:959	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	4	66	theme	AAB	915:917	arg1	strain					919:924	an AAB strain	912:924	an AAB strain (Komagataeibacter intermedius V-05)	912:960	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	4	67	from	effects	832:838	arg1	media					885:889	the culture media	873:889	the culture media	873:889	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	5	68	theme	Burman	1054:1059	arg1	design					1071:1076	a Plackett and Burman factorial design	1039:1076	a Plackett and Burman factorial design	1039:1076	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	4	69	theme	different	720:728	arg1	nutrients					730:738	different nutrients	720:738	different nutrients as nitrogen source	720:757	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	0	70	theme	Amino	33:37	arg1	Acids					39:43	Amino Acids	33:43	Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius	33:109	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	1	71	theme	several	273:279	arg1	applications					281:292	several applications	273:292	several applications	273:292	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	2	72	theme	chemical	397:404	arg1	elements					406:413	chemical elements	397:413	chemical elements	397:413	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	5	73	theme	required	1155:1162	arg1	acid					1170:1173	each required amino acid	1150:1173	each required amino acid	1150:1173	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	5	74	theme	amino	982:986	arg1	acids					988:992	nineteen amino acids	973:992	nineteen amino acids	973:992	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	9	75	theme	validation	1736:1745	arg1	steps					1747:1751	validation steps	1736:1751	validation steps	1736:1751	After optimization and validation steps, a production level of 3.02 g L-1 was achieved.
35687307	0	76	theme	Cellulose	58:66	arg1	Production					68:77	Bacterial Cellulose Production	48:77	Bacterial Cellulose Production by Komagataeibacter intermedius	48:109	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	1	77	theme	medical	301:307	arg1	industries					335:344	the medical, pharmaceutical, and food industries	297:344	the medical, pharmaceutical, and food industries	297:344	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	7	78	theme	positive	1486:1493	arg1	effect					1495:1500	a significant positive effect	1472:1500	a significant positive effect	1472:1500	Three amino acids had a significant positive effect and were required: aspartic acid (1.5 g L-1), phenylalanine (1.5 g L-1), and serine (3.0 g L-1).
35687307	3	79	theme	appropriate	660:670	arg1	form					672:675	the appropriate form	656:675	the appropriate form	656:675	So, the knowledge of the nutritional needs of microorganisms that have important industrial applications is necessary for the nutrients to be supplied in the appropriate form and amount.
35687307	0	80	with	Supplementation	12:26	arg1	Acids					39:43	Amino Acids	33:43	Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius	33:109	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	5	81	theme	optimal	1124:1130	arg1	concentrations					1132:1145	the optimal concentrations	1120:1145	the optimal concentrations of each required amino acid	1120:1173	For this, nineteen amino acids were tested, selected, and optimized through a Plackett and Burman factorial design and central composite design to determine the optimal concentrations of each required amino acid.
35687307	9	82	theme	production	1756:1765	arg1	level					1767:1771	a production level	1754:1771	a production level of 3.02 g L-1	1754:1785	After optimization and validation steps, a production level of 3.02 g L-1 was achieved.
35687307	4	83	theme	different	773:781	arg1	effects					793:799	different metabolic effects	773:799	different metabolic effects	773:799	Considering that the choice of different nutrients as nitrogen source can result in different metabolic effects, this work aimed to verify the effects of amino acid supplementation in the culture media for BC production by an AAB strain (Komagataeibacter intermedius V-05).
35687307	1	84	theme	acetic	237:242	arg1	AAB					259:261	AAB	259:261	AAB	259:261	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	1	84	theme	acetic	237:242	arg1	bacteria					249:256	acetic acid bacteria	237:256	acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries	237:344	Bacterial cellulose (BC) is a biopolymer mainly produced by acetic acid bacteria (AAB) that has several applications in the medical, pharmaceutical, and food industries.
35687307	0	85	dep	Supplementation	12:26	arg1	Application					132:142	Application	132:142	Application	132:142	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	0	85	dep	Supplementation	12:26	arg1	Analysis					119:126	Effect Analysis	112:126	Effect Analysis	112:126	Nutritional Supplementation with Amino Acids on Bacterial Cellulose Production by Komagataeibacter intermedius: Effect Analysis and Application of Response Surface Methodology.
35687307	10	86	theme	HS	1973:1974	arg1	medium					1976:1981	standard HS medium	1964:1981	standard HS medium	1964:1981	Membranes produced from optimized media by this strain presented lower crystallinity index but greater thermal and hydrophilic properties than those produced from standard HS medium.
35687307	2	87	theme	other	350:354	arg1	organisms					363:371	other living organisms	350:371	other living organisms	350:371	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35687307	2	87	theme	other	350:354	arg1	sources					386:392	sources	386:392	sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production	386:499	As other living organisms, AAB require sources of chemical elements and nutrients, which are essential for their multiplication and metabolite production.
35604937	10	0	from	able	1930:1933	arg1	case					1795:1798	the case	1791:1798	the case of individual bacterial strains	1791:1830	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	1	theme	anaerobic	1983:1991	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	1	theme	anaerobic	1983:1991	arg1	strains					1993:1999	Gram-positive facultatitive anaerobic strains	1955:1999	Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis	1955:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	1	theme	anaerobic	1983:1991	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	1	theme	anaerobic	1983:1991	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	1	theme	anaerobic	1983:1991	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	2	theme	facultatitive	1969:1981	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	2	theme	facultatitive	1969:1981	arg1	strains					1993:1999	Gram-positive facultatitive anaerobic strains	1955:1999	Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis	1955:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	2	theme	facultatitive	1969:1981	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	2	theme	facultatitive	1969:1981	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	2	theme	facultatitive	1969:1981	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	0	3	theme	foot	172:175	arg1	infections					177:186	diabetic foot infections	163:186	diabetic foot infections	163:186	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	5	4	attach	derived	864:870	arg2	strains					856:862	individual bacterial strains	835:862	individual bacterial strains derived from DFIs.Methodology	835:892	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	5	4	attach	derived	864:870	arg1	DFIs.Methodology					877:892	DFIs.Methodology	877:892	DFIs.Methodology	877:892	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	9	5	theme	tissue	1716:1721	arg1	homogenates					1723:1733	all polymicrobial tissue homogenates	1698:1733	all polymicrobial tissue homogenates tested	1698:1740	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	11	6	theme	in	2479:2480	arg1	zone					2488:2491	in vitro zone	2479:2491	in vitro zone of inhibition tests	2479:2511	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	5	7	theme	loaded	718:723	arg1	beads					741:745	antibiotic loaded calcium sulfate beads	707:745	antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK)	707:791	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	6	8	theme	foot	955:958	arg1	ulcers					960:965	infected diabetic foot ulcers	937:965	infected diabetic foot ulcers	937:965	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	2	9	theme	diabetes-related	256:271	arg1	hospitalisation					273:287	diabetes-related hospitalisation	256:287	diabetes-related hospitalisation	256:287	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	6	10	theme	infected	937:944	arg1	ulcers					960:965	infected diabetic foot ulcers	937:965	infected diabetic foot ulcers	937:965	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	12	11	theme	further	2571:2577	arg1	research					2579:2586	further research	2571:2586	further research	2571:2586	These results show promise for clinical application, but further research and clinical studies are required.
35604937	5	12	theme	sulfate	733:739	arg1	beads					741:745	antibiotic loaded calcium sulfate beads	707:745	antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK)	707:791	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	8	13	attach	isolated	1303:1310	arg1	tissue					1330:1335	the infected tissue	1317:1335	the infected tissue	1317:1335	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	8	13	attach	isolated	1303:1310	arg2	strains					1295:1301	individual bacterial strains	1274:1301	individual bacterial strains isolated from the infected tissue	1274:1335	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	6	14	theme	agar	1029:1032	arg1	CBA					1035:1037	Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	1014:1080	CBA	1035:1037	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	12	15	theme	clinical	2592:2599	arg1	studies					2601:2607	clinical studies	2592:2607	clinical studies	2592:2607	These results show promise for clinical application, but further research and clinical studies are required.
35604937	8	16	theme	bacterial	1285:1293	arg1	strains					1295:1301	individual bacterial strains	1274:1301	individual bacterial strains isolated from the infected tissue	1274:1335	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	5	17	theme	Rapid	757:761	arg1	Cure					763:766	Rapid Cure	757:766	Stimulan Rapid Cure	748:766	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	10	18	theme	bacterial	1814:1822	arg1	strains					1824:1830	individual bacterial strains	1803:1830	individual bacterial strains	1803:1830	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	6	19	theme	Columbia	1014:1021	arg1	CBA					1035:1037	Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	1014:1080	CBA	1035:1037	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	8	20	theme	gentamicin	1477:1486	arg1	combination					1447:1457	a combination	1445:1457	a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1445:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	10	21	theme	antibiotics	2234:2244	arg1	one					2223:2225	one	2223:2225	one	2223:2225	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	21	theme	antibiotics	2234:2244	arg1	antibiotics					2234:2244	the antibiotics	2230:2244	the antibiotics	2230:2244	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	0	22	theme	individual	121:130	arg1	strains					142:148	individual bacterial strains	121:148	individual bacterial strains	121:148	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	0	23	theme	diabetic	163:170	arg1	infections					177:186	diabetic foot infections	163:186	diabetic foot infections	163:186	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	10	24	theme	facultative	2072:2082	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	24	theme	facultative	2072:2082	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	24	theme	facultative	2072:2082	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	24	theme	facultative	2072:2082	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	24	theme	facultative	2072:2082	arg1	strains					2094:2100	Gram-negative facultative anaerobic strains	2058:2100	Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa	2058:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	7	25	theme	Calcium	1083:1089	arg1	beads					1099:1103	Calcium sulfate beads	1083:1103	Calcium sulfate beads containing a combination of vancomycin and gentamicin	1083:1157	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	7	26	theme	gentamicin	1148:1157	arg1	combination					1118:1128	a combination	1116:1128	a combination of vancomycin and gentamicin	1116:1157	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	10	27	from	case	1795:1798	arg1	able					1930:1933	able	1930:1933	able	1930:1933	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	8	28	theme	calcium	1338:1344	arg1	beads					1354:1358	calcium sulfate beads	1338:1358	calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1338:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	6	29	theme	anaerobe	1055:1062	arg1	plates					1075:1080	Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	1014:1080	plates	1075:1080	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	5	30	dep	Stimulan	748:755	arg1	Cure					763:766	Rapid Cure	757:766	Stimulan Rapid Cure	748:766	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	11	31	from	efficacy	2392:2399	arg1	zone					2488:2491	in vitro zone	2479:2491	in vitro zone of inhibition tests	2479:2511	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	5	32	theme	DFI	815:817	arg1	communities					819:829	polymicrobial DFI communities	801:829	polymicrobial DFI communities	801:829	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	9	33	theme	sulfate	1586:1592	arg1	beads					1594:1598	Calcium sulfate beads	1578:1598	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin	1578:1653	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	5	34	dep	beads	741:745	arg1	UK					789:790	UK	789:790	UK	789:790	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	5	34	dep	beads	741:745	arg1	Stimulan					748:755	Stimulan	748:755	Stimulan	748:755	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	4	35	theme	loaded	545:550	arg1	beads					552:556	Antibiotic loaded beads	534:556	Antibiotic loaded beads	534:556	Antibiotic loaded beads could inhibit the growth of polymicrobial DFI communities with differing compositions in vitro.Aim.
35604937	0	36	theme	bacterial	132:140	arg1	strains					142:148	individual bacterial strains	121:148	individual bacterial strains	121:148	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	7	37	theme	inhibition	1238:1247	arg1	zones					1229:1233	zones	1229:1233	zones of inhibition (ZOI)	1229:1253	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	10	38	theme	obligate	2137:2144	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	38	theme	obligate	2137:2144	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	38	theme	obligate	2137:2144	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	38	theme	obligate	2137:2144	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	38	theme	obligate	2137:2144	arg1	strains					2156:2162	obligate anaerobic strains	2137:2162	obligate anaerobic strains such as Finegoldia magna	2137:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	4	39	from	compositions	631:642	arg1	vitro.Aim					647:655	vitro.Aim	647:655	vitro.Aim	647:655	Antibiotic loaded beads could inhibit the growth of polymicrobial DFI communities with differing compositions in vitro.Aim.
35604937	3	40	from	ischaemia	372:380	arg1	limb					398:401	the affected limb	385:401	the affected limb	385:401	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	6	41	theme	CBA	1035:1037	arg1	surface					1003:1009	the surface	999:1009	the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	999:1080	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	8	42	contain	containing	1360:1369	arg2	vancomycin					1371:1380	vancomycin	1371:1380	vancomycin	1371:1380	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	8	42	contain	containing	1360:1369	arg1	beads					1354:1358	calcium sulfate beads	1338:1358	calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1338:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	8	42	contain	containing	1360:1369	arg2	gentamicin					1383:1392	gentamicin	1383:1392	gentamicin	1383:1392	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	8	42	contain	containing	1360:1369	arg2	beads					1428:1432	beads	1428:1432	beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1428:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	8	42	contain	containing	1360:1369	arg2	flucloxacillin					1395:1408	flucloxacillin	1395:1408	flucloxacillin	1395:1408	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	8	42	contain	containing	1360:1369	arg2	rifampicin					1413:1422	rifampicin	1413:1422	rifampicin	1413:1422	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	11	43	theme	inhibition	2496:2505	arg1	tests					2507:2511	inhibition tests	2496:2511	inhibition tests	2496:2511	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	3	44	theme	biofilms	434:441	arg1	ischaemia					372:380	ischaemia	372:380	ischaemia in the affected limb	372:401	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	3	44	theme	biofilms	434:441	arg1	occurrence					464:473	increasingly the occurrence	447:473	increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement	447:531	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	3	44	theme	biofilms	434:441	arg1	presence					408:415	the presence	404:415	the presence of polymicrobial biofilms	404:441	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	9	45	dep	40 mm	1781:1785	arg1	to					1778:1779	to	1778:1779	to	1778:1779	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	5	46	dep	in	686:687	arg1	vitro					689:693	vitro	689:693	vitro	689:693	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	10	47	contain	containing	1839:1848	arg2	combinations					1850:1861	combinations	1850:1861	combinations of vancomycin and gentamicin or flucloxacillin and rifampicin	1850:1923	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	47	contain	containing	1839:1848	arg1	beads					1833:1837	beads	1833:1837	beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin	1833:1923	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	0	48	theme	In	0:1	arg1	efficacy					9:16	In vitro efficacy	0:16	In vitro efficacy of antibiotic	0:30	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	0	49	dep	Stimulan	62:69	arg1	Cure					77:80	Rapid Cure	71:80	Stimulan Rapid Cure	62:80	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	11	50	theme	sulfate	2316:2322	arg1	beads					2324:2328	calcium sulfate beads	2308:2328	calcium sulfate beads containing a combination of two antibiotics	2308:2372	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	10	51	theme	gentamicin	1881:1890	arg1	combinations					1850:1861	combinations	1850:1861	combinations of vancomycin and gentamicin or flucloxacillin and rifampicin	1850:1923	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	52	from	resistance	2209:2218	arg1	combination					2253:2263	the combination	2249:2263	the combination	2249:2263	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	7	53	theme	agar	1198:1201	arg1	surface					1183:1189	the surface	1179:1189	the surface of the agar	1179:1201	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	0	54	dep	beads	55:59	arg1	Stimulan					62:69	Stimulan	62:69	Stimulan	62:69	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	8	55	contain	containing	1434:1443	arg1	beads					1428:1432	beads	1428:1432	beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1428:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	8	55	contain	containing	1434:1443	arg2	combination					1447:1457	a combination	1445:1457	a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1445:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	4	56	theme	DFI	600:602	arg1	communities					604:614	polymicrobial DFI communities	586:614	polymicrobial DFI communities	586:614	Antibiotic loaded beads could inhibit the growth of polymicrobial DFI communities with differing compositions in vitro.Aim.
35604937	7	57	contain	containing	1105:1114	arg1	beads					1099:1103	Calcium sulfate beads	1083:1103	Calcium sulfate beads containing a combination of vancomycin and gentamicin	1083:1157	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	7	57	contain	containing	1105:1114	arg2	combination					1118:1128	a combination	1116:1128	a combination of vancomycin and gentamicin	1116:1157	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	0	58	theme	antibiotic	21:30	arg1	efficacy					9:16	In vitro efficacy	0:16	In vitro efficacy of antibiotic	0:30	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	11	59	theme	local	2295:2299	arg1	use					2301:2303	The local use	2291:2303	The local use of calcium sulfate beads containing a combination of two antibiotics	2291:2372	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	3	60	theme	bacteria.Hypothesis/Gap	499:521	arg1	statement					523:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	5	61	theme	bacterial	846:854	arg1	strains					856:862	individual bacterial strains	835:862	individual bacterial strains derived from DFIs.Methodology	835:892	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	11	62	theme	bacterial	2458:2466	arg1	strains					2468:2474	individual DFI bacterial strains	2443:2474	individual DFI bacterial strains	2443:2474	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	2	63	theme	foot	212:215	arg1	reason					245:250	the main reason	236:250	the main reason for diabetes-related hospitalisation	236:287	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	63	theme	foot	212:215	arg1	DFI					228:230	DFI	228:230	DFI	228:230	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	63	theme	foot	212:215	arg1	infection					217:225	Diabetic foot infection	203:225	Diabetic foot infection (DFI)	203:231	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	64	theme	amputation	330:339	arg1	cause					304:308	a major cause	296:308	a major cause of diabetes-related amputation	296:339	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	64	theme	amputation	330:339	arg1	reason					245:250	the main reason	236:250	the main reason for diabetes-related hospitalisation	236:287	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	64	theme	amputation	330:339	arg1	infection					217:225	Diabetic foot infection	203:225	Diabetic foot infection (DFI)	203:231	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	6	65	theme	Debrided	895:902	arg1	tissue					904:909	Debrided tissue	895:909	Debrided tissue obtained from the base of infected diabetic foot ulcers	895:965	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	9	66	theme	bacterial	1676:1684	arg1	growth					1686:1691	bacterial growth	1676:1691	bacterial growth	1676:1691	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	11	67	theme	individual	2443:2452	arg1	strains					2468:2474	individual DFI bacterial strains	2443:2474	individual DFI bacterial strains	2443:2474	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	11	68	theme	DFI	2423:2425	arg1	communities					2427:2437	polymicrobial DFI communities	2409:2437	polymicrobial DFI communities	2409:2437	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	5	69	theme	individual	835:844	arg1	strains					856:862	individual bacterial strains	835:862	individual bacterial strains derived from DFIs.Methodology	835:892	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	0	70	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	5	71	theme	polymicrobial	801:813	arg1	communities					819:829	polymicrobial DFI communities	801:829	polymicrobial DFI communities	801:829	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	9	72	theme	polymicrobial	1702:1714	arg1	homogenates					1723:1733	all polymicrobial tissue homogenates	1698:1733	all polymicrobial tissue homogenates tested	1698:1740	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	10	73	with	ZOI	1946:1948	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	73	with	ZOI	1946:1948	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	73	with	ZOI	1946:1948	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	73	with	ZOI	1946:1948	arg1	strains					2094:2100	Gram-negative facultative anaerobic strains	2058:2100	Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa	2058:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	73	with	ZOI	1946:1948	arg1	strains					1993:1999	Gram-positive facultatitive anaerobic strains	1955:1999	Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis	1955:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	73	with	ZOI	1946:1948	arg1	strains					2156:2162	obligate anaerobic strains	2137:2162	obligate anaerobic strains such as Finegoldia magna	2137:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	73	with	ZOI	1946:1948	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	74	theme	Gram-positive	1955:1967	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	74	theme	Gram-positive	1955:1967	arg1	strains					1993:1999	Gram-positive facultatitive anaerobic strains	1955:1999	Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis	1955:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	74	theme	Gram-positive	1955:1967	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	74	theme	Gram-positive	1955:1967	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	74	theme	Gram-positive	1955:1967	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	5	75	theme	antibiotic	707:716	arg1	beads					741:745	antibiotic loaded calcium sulfate beads	707:745	antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK)	707:791	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	10	76	theme	individual	1803:1812	arg1	strains					1824:1830	individual bacterial strains	1803:1830	individual bacterial strains	1803:1830	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	6	77	theme	ulcers	960:965	arg1	base					929:932	the base	925:932	the base of infected diabetic foot ulcers	925:965	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	11	78	theme	high	2387:2390	arg1	efficacy					2392:2399	high efficacy	2387:2399	high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests	2387:2511	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	7	79	theme	vancomycin	1133:1142	arg1	combination					1118:1128	a combination	1116:1128	a combination of vancomycin and gentamicin	1116:1157	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	0	80	theme	calcium	39:45	arg1	beads					55:59	calcium sulfate beads	39:59	calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections	39:186	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	6	81	theme	diabetic	946:953	arg1	ulcers					960:965	infected diabetic foot ulcers	937:965	infected diabetic foot ulcers	937:965	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	5	82	theme	in	686:687	arg1	efficacy					695:702	the in vitro efficacy	682:702	the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology	682:892	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	11	83	dep	in	2479:2480	arg1	vitro					2482:2486	vitro	2482:2486	vitro	2482:2486	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	9	84	theme	ZOI	1748:1750	arg1	diameters					1752:1760	ZOI diameters	1748:1760	ZOI diameters ranging from 15 to 40 mm	1748:1785	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	2	85	theme	major	298:302	arg1	cause					304:308	a major cause	296:308	a major cause of diabetes-related amputation	296:339	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	6	86	theme	blood	1023:1027	arg1	CBA					1035:1037	Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	1014:1080	CBA	1035:1037	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	3	87	attach	presence	408:415	arg2	statement					523:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	3	87	attach	presence	408:415	arg1	limb					398:401	the affected limb	385:401	the affected limb	385:401	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	3	87	attach	presence	408:415	arg2	biofilms					434:441	polymicrobial biofilms	420:441	polymicrobial biofilms	420:441	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	11	88	theme	polymicrobial	2409:2421	arg1	communities					2427:2437	polymicrobial DFI communities	2409:2437	polymicrobial DFI communities	2409:2437	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	8	89	theme	individual	1274:1283	arg1	strains					1295:1301	individual bacterial strains	1274:1301	individual bacterial strains isolated from the infected tissue	1274:1335	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	7	90	theme	sulfate	1091:1097	arg1	beads					1099:1103	Calcium sulfate beads	1083:1103	Calcium sulfate beads containing a combination of vancomycin and gentamicin	1083:1157	Calcium sulfate beads containing a combination of vancomycin and gentamicin were then placed on the surface of the agar and following incubation, zones of inhibition (ZOI) were measured.
35604937	10	91	theme	vancomycin	1866:1875	arg1	combinations					1850:1861	combinations	1850:1861	combinations of vancomycin and gentamicin or flucloxacillin and rifampicin	1850:1923	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	92	theme	anaerobic	2084:2092	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	92	theme	anaerobic	2084:2092	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	92	theme	anaerobic	2084:2092	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	92	theme	anaerobic	2084:2092	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	92	theme	anaerobic	2084:2092	arg1	strains					2094:2100	Gram-negative facultative anaerobic strains	2058:2100	Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa	2058:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	5	93	theme	calcium	725:731	arg1	beads					741:745	antibiotic loaded calcium sulfate beads	707:745	antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK)	707:791	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	10	94	theme	Gram-negative	2058:2070	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	94	theme	Gram-negative	2058:2070	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	94	theme	Gram-negative	2058:2070	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	94	theme	Gram-negative	2058:2070	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	94	theme	Gram-negative	2058:2070	arg1	strains					2094:2100	Gram-negative facultative anaerobic strains	2058:2100	Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa	2058:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	5	95	theme	beads	741:745	arg1	efficacy					695:702	the in vitro efficacy	682:702	the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology	682:892	This study investigates the in vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure, Biocomposites Ltd., UK) against polymicrobial DFI communities and individual bacterial strains derived from DFIs.Methodology.
35604937	10	96	theme	strains	1824:1830	arg1	case					1795:1798	the case	1791:1798	the case of individual bacterial strains	1791:1830	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	6	97	theme	FAA	1070:1072	arg1	plates					1075:1080	Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	1014:1080	plates	1075:1080	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	8	98	theme	infected	1321:1328	arg1	tissue					1330:1335	the infected tissue	1317:1335	the infected tissue	1317:1335	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	3	99	theme	antibiotic	478:487	arg1	statement					523:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	2	100	theme	main	240:243	arg1	reason					245:250	the main reason	236:250	the main reason for diabetes-related hospitalisation	236:287	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	100	theme	main	240:243	arg1	infection					217:225	Diabetic foot infection	203:225	Diabetic foot infection (DFI)	203:231	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	6	101	theme	agar	1064:1067	arg1	plates					1075:1080	Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	1014:1080	plates	1075:1080	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	4	102	theme	Antibiotic	534:543	arg1	beads					552:556	Antibiotic loaded beads	534:556	Antibiotic loaded beads	534:556	Antibiotic loaded beads could inhibit the growth of polymicrobial DFI communities with differing compositions in vitro.Aim.
35604937	0	103	theme	Rapid	71:75	arg1	Cure					77:80	Rapid Cure	71:80	Stimulan Rapid Cure	62:80	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	9	104	theme	Calcium	1578:1584	arg1	beads					1594:1598	Calcium sulfate beads	1578:1598	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin	1578:1653	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	6	105	theme	fastidious	1044:1053	arg1	plates					1075:1080	Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	1014:1080	plates	1075:1080	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	11	106	theme	beads	2324:2328	arg1	use					2301:2303	The local use	2291:2303	The local use of calcium sulfate beads containing a combination of two antibiotics	2291:2372	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	11	107	theme	tests	2507:2511	arg1	zone					2488:2491	in vitro zone	2479:2491	in vitro zone of inhibition tests	2479:2511	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	8	108	theme	sulfate	1346:1352	arg1	beads					1354:1358	calcium sulfate beads	1338:1358	calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1338:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
35604937	10	109	theme	anaerobic	2146:2154	arg1	faecalis					2048:2055	Enterococcus faecalis	2035:2055	Enterococcus faecalis	2035:2055	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	109	theme	anaerobic	2146:2154	arg1	aureus					2024:2029	Staphylococcus aureus	2009:2029	Staphylococcus aureus	2009:2029	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	109	theme	anaerobic	2146:2154	arg1	aeruginosa					2122:2131	Pseudomonas aeruginosa	2110:2131	Pseudomonas aeruginosa	2110:2131	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	109	theme	anaerobic	2146:2154	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	10	109	theme	anaerobic	2146:2154	arg1	strains					2156:2162	obligate anaerobic strains	2137:2162	obligate anaerobic strains such as Finegoldia magna	2137:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	3	110	from	presence	408:415	arg1	limb					398:401	the affected limb	385:401	the affected limb	385:401	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	11	111	theme	antibiotics	2362:2372	arg1	combination					2343:2353	a combination	2341:2353	a combination of two antibiotics	2341:2372	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	2	112	theme	diabetes-related	313:328	arg1	amputation					330:339	diabetes-related amputation	313:339	diabetes-related amputation	313:339	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	0	113	theme	polymicrobial	91:103	arg1	communities					105:115	polymicrobial communities	91:115	polymicrobial communities	91:115	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	3	114	theme	affected	389:396	arg1	limb					398:401	the affected limb	385:401	the affected limb	385:401	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	4	115	theme	polymicrobial	586:598	arg1	communities					604:614	polymicrobial DFI communities	586:614	polymicrobial DFI communities	586:614	Antibiotic loaded beads could inhibit the growth of polymicrobial DFI communities with differing compositions in vitro.Aim.
35604937	3	116	theme	polymicrobial	420:432	arg1	biofilms					434:441	polymicrobial biofilms	420:441	polymicrobial biofilms	420:441	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	9	117	theme	vancomycin	1629:1638	arg1	combination					1614:1624	a combination	1612:1624	a combination of vancomycin and gentamicin	1612:1653	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	0	118	attach	derived	150:156	arg2	communities					105:115	polymicrobial communities	91:115	polymicrobial communities	91:115	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	0	118	attach	derived	150:156	arg1	infections					177:186	diabetic foot infections	163:186	diabetic foot infections	163:186	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	4	119	theme	communities	604:614	arg1	growth					576:581	the growth	572:581	the growth of polymicrobial DFI communities	572:614	Antibiotic loaded beads could inhibit the growth of polymicrobial DFI communities with differing compositions in vitro.Aim.
35604937	11	120	contain	containing	2330:2339	arg1	beads					2324:2328	calcium sulfate beads	2308:2328	calcium sulfate beads containing a combination of two antibiotics	2308:2372	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	11	120	contain	containing	2330:2339	arg2	combination					2343:2353	a combination	2341:2353	a combination of two antibiotics	2341:2372	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	9	121	theme	gentamicin	1644:1653	arg1	combination					1614:1624	a combination	1612:1624	a combination of vancomycin and gentamicin	1612:1653	Calcium sulfate beads loaded with a combination of vancomycin and gentamicin were able to inhibit bacterial growth from all polymicrobial tissue homogenates tested, with ZOI diameters ranging from 15 to 40 mm.
35604937	6	122	theme	plates	1075:1080	arg1	surface					1003:1009	the surface	999:1009	the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates	999:1080	Debrided tissue obtained from the base of infected diabetic foot ulcers was homogenised and spread over the surface of Columbia blood agar (CBA) and fastidious anaerobe agar (FAA) plates.
35604937	11	123	theme	calcium	2308:2314	arg1	beads					2324:2328	calcium sulfate beads	2308:2328	calcium sulfate beads containing a combination of two antibiotics	2308:2372	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	12	124	theme	clinical	2545:2552	arg1	application					2554:2564	clinical application	2545:2564	clinical application	2545:2564	These results show promise for clinical application, but further research and clinical studies are required.
35604937	0	125	theme	sulfate	47:53	arg1	beads					55:59	calcium sulfate beads	39:59	calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections	39:186	In vitro efficacy of antibiotic loaded calcium sulfate beads (Stimulan Rapid Cure) against polymicrobial communities and individual bacterial strains derived from diabetic foot infections.
35604937	3	126	from	occurrence	464:473	arg1	limb					398:401	the affected limb	385:401	the affected limb	385:401	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	3	127	theme	statement	523:531	arg1	ischaemia					372:380	ischaemia	372:380	ischaemia in the affected limb	372:401	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	3	127	theme	statement	523:531	arg1	occurrence					464:473	increasingly the occurrence	447:473	increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement	447:531	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	3	127	theme	statement	523:531	arg1	presence					408:415	the presence	404:415	the presence of polymicrobial biofilms	404:441	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	10	128	theme	Finegoldia	2172:2181	arg1	magna					2183:2187	Finegoldia magna	2172:2187	Finegoldia magna	2172:2187	In the case of individual bacterial strains, beads containing combinations of vancomycin and gentamicin or flucloxacillin and rifampicin were able to produce ZOI with Gram-positive facultatitive anaerobic strains such as Staphylococcus aureus and Enterococcus faecalis, Gram-negative facultative anaerobic strains such as Pseudomonas aeruginosa and obligate anaerobic strains such as Finegoldia magna even where acquired resistance to one of the antibiotics in the combination was evidenced.Conclusion.
35604937	2	129	theme	Diabetic	203:210	arg1	reason					245:250	the main reason	236:250	the main reason for diabetes-related hospitalisation	236:287	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	129	theme	Diabetic	203:210	arg1	DFI					228:230	DFI	228:230	DFI	228:230	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	2	129	theme	Diabetic	203:210	arg1	infection					217:225	Diabetic foot infection	203:225	Diabetic foot infection (DFI)	203:231	Diabetic foot infection (DFI) is the main reason for diabetes-related hospitalisation and is a major cause of diabetes-related amputation.
35604937	3	130	theme	resistant	489:497	arg1	statement					523:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	antibiotic resistant bacteria.Hypothesis/Gap statement	478:531	DFIs are often complicated by ischaemia in the affected limb, the presence of polymicrobial biofilms and increasingly the occurrence of antibiotic resistant bacteria.Hypothesis/Gap statement.
35604937	11	131	theme	DFI	2454:2456	arg1	strains					2468:2474	individual DFI bacterial strains	2443:2474	individual DFI bacterial strains	2443:2474	The local use of calcium sulfate beads containing a combination of two antibiotics demonstrated high efficacy against polymicrobial DFI communities and individual DFI bacterial strains in in vitro zone of inhibition tests.
35604937	8	132	theme	vancomycin	1462:1471	arg1	combination					1447:1457	a combination	1445:1457	a combination of vancomycin and gentamicin or flucloxacillin and rifampicin	1445:1519	For individual bacterial strains isolated from the infected tissue, calcium sulfate beads containing vancomycin, gentamicin, flucloxacillin or rifampicin and beads containing a combination of vancomycin and gentamicin or flucloxacillin and rifampicin were tested for their ability to inhibit growth.Results.
36528632	9	0	theme	high	1246:1249	arg1	degree					1261:1266	the high precision degree	1242:1266	the high precision degree of both models	1242:1281	Despite the high precision degree of both models, ANN was supreme in the prediction of CNPs biosynthesis compared to CCFCD.
36528632	11	1	theme	Pectobacterium	1551:1564	arg1	carotovorum					1566:1576	Pectobacterium carotovorum	1551:1576	Pectobacterium carotovorum	1551:1576	CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot.
36528632	13	2	theme	eco-friendly	1844:1855	arg1	approach					1857:1864	an eco-friendly approach	1841:1864	an eco-friendly approach	1841:1864	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	13	2	theme	eco-friendly	1844:1855	arg1	NEAE-83					1830:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	1	3	theme	traditional	315:325	arg1	protocols					349:357	traditional chemical and physical protocols	315:357	traditional chemical and physical protocols	315:357	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	0	4	theme	chitosan	93:100	arg1	optimization					55:66	artificial intelligence-based optimization	25:66	artificial intelligence-based optimization	25:66	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	0	4	theme	chitosan	93:100	arg1	characterization					73:88	characterization	73:88	characterization	73:88	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	0	4	theme	chitosan	93:100	arg1	biosynthesis					11:22	Innovative biosynthesis	0:22	Innovative biosynthesis	0:22	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	4	5	theme	Streptomyces	590:601	arg1	It					569:570	It	569:570	It	569:570	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	4	5	theme	Streptomyces	590:601	arg1	strain					615:620	Streptomyces microflavus strain NEAE-83	590:628	Streptomyces microflavus strain NEAE-83	590:628	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	6	6	theme	CNPs	838:841	arg1	biosynthesis					843:854	CNPs biosynthesis	838:854	CNPs biosynthesis	838:854	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	1	7	theme	Microbial-based	211:225	arg1	strategy					227:234	Microbial-based strategy	211:234	Microbial-based strategy in nanotechnology	211:252	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	12	8	theme	potential	1641:1649	arg1	application					1651:1661	its potential application	1637:1661	its potential application for controlling the destructive potato soft rot diseases	1637:1718	These results suggested its potential application for controlling the destructive potato soft rot diseases.
36528632	1	9	theme	chemical	327:334	arg1	protocols					349:357	traditional chemical and physical protocols	315:357	traditional chemical and physical protocols	315:357	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	0	10	theme	Streptomyces	119:130	arg1	microflavus					132:142	Streptomyces microflavus	119:142	Streptomyces microflavus	119:142	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	13	11	theme	process	1903:1909	arg1	optimization					1870:1881	optimization	1870:1881	optimization of the biosynthesis process by artificial intelligence	1870:1936	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	13	11	theme	process	1903:1909	arg1	approach					1857:1864	an eco-friendly approach	1841:1864	an eco-friendly approach	1841:1864	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	13	11	theme	process	1903:1909	arg1	NEAE-83					1830:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	10	12	theme	prediction	1375:1384	arg1	efficacy					1386:1393	a higher prediction efficacy	1366:1393	a higher prediction efficacy	1366:1393	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	12	13	theme	soft	1702:1705	arg1	diseases					1711:1718	the destructive potato soft rot diseases	1679:1718	the destructive potato soft rot diseases	1679:1718	These results suggested its potential application for controlling the destructive potato soft rot diseases.
36528632	2	14	theme	current	364:370	arg1	study					372:376	The current study	360:376	The current study	360:376	The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp.
36528632	10	15	contain	had	1362:1364	arg2	MDA					1426:1428	MDA	1426:1428	MDA	1426:1428	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	15	contain	had	1362:1364	arg2	values					1412:1417	lower error values	1400:1417	lower error values (RMSE, MDA, and SSE)	1400:1438	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	15	contain	had	1362:1364	arg1	ANN					1358:1360	ANN	1358:1360	ANN	1358:1360	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	15	contain	had	1362:1364	arg2	SSE					1435:1437	SSE	1435:1437	SSE	1435:1437	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	15	contain	had	1362:1364	arg2	RMSE					1420:1423	RMSE	1420:1423	RMSE	1420:1423	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	15	contain	had	1362:1364	arg2	efficacy					1386:1393	a higher prediction efficacy	1366:1393	a higher prediction efficacy	1366:1393	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	13	16	theme	first	1733:1737	arg1	report					1739:1744	the first report	1729:1744	the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence	1729:1936	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	13	16	theme	first	1733:1737	arg1	This					1721:1724	This	1721:1724	This	1721:1724	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	10	17	theme	higher	1368:1373	arg1	efficacy					1386:1393	a higher prediction efficacy	1366:1393	a higher prediction efficacy	1366:1393	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	1	18	theme	physical	340:347	arg1	protocols					349:357	traditional chemical and physical protocols	315:357	traditional chemical and physical protocols	315:357	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	11	19	theme	potato	1596:1601	arg1	rot					1608:1610	the potato soft rot	1592:1610	the potato soft rot	1592:1610	CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot.
36528632	7	20	theme	a 12 h	1065:1070	arg1	period					1083:1088	a 12 h incubation period	1065:1088	a 12 h incubation period	1065:1088	The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period.
36528632	11	21	theme	soft	1603:1606	arg1	rot					1608:1610	the potato soft rot	1592:1610	the potato soft rot	1592:1610	CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot.
36528632	2	22	theme	pioneer	465:471	arg1	sp					486:487	a pioneer Streptomyces sp	463:487	a pioneer Streptomyces sp	463:487	The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp.
36528632	5	23	theme	zeta	797:800	arg1	potential					802:810	zeta potential	797:810	zeta potential	797:810	CNPs were characterized by SEM, TEM, EDXS, zeta potential, FTIR, XRD, TGA, and DSC.
36528632	9	24	theme	models	1276:1281	arg1	degree					1261:1266	the high precision degree	1242:1266	the high precision degree of both models	1242:1281	Despite the high precision degree of both models, ANN was supreme in the prediction of CNPs biosynthesis compared to CCFCD.
36528632	7	25	theme	incubation	1072:1081	arg1	period					1083:1088	a 12 h incubation period	1065:1088	a 12 h incubation period	1065:1088	The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period.
36528632	7	26	theme	CNPs	967:970	arg1	yield					958:962	The highest yield	946:962	The highest yield of CNPs (9.41 mg/mL)	946:983	The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period.
36528632	13	27	theme	strain	1823:1828	arg1	approach					1857:1864	an eco-friendly approach	1841:1864	an eco-friendly approach	1841:1864	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	13	27	theme	strain	1823:1828	arg1	NEAE-83					1830:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	6	28	theme	composite	920:928	arg1	CCFCD					938:942	CCFCD	938:942	CCFCD	938:942	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	6	28	theme	composite	920:928	arg1	design					930:935	face-centered central composite design	898:935	face-centered central composite design (CCFCD)	898:943	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	6	28	theme	composite	920:928	arg1	a mathematical model					876:895	a mathematical model	876:895	a mathematical model	876:895	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	10	29	theme	lower	1400:1404	arg1	MDA					1426:1428	MDA	1426:1428	MDA	1426:1428	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	29	theme	lower	1400:1404	arg1	SSE					1435:1437	SSE	1435:1437	SSE	1435:1437	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	29	theme	lower	1400:1404	arg1	RMSE					1420:1423	RMSE	1420:1423	RMSE	1420:1423	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	29	theme	lower	1400:1404	arg1	values					1412:1417	lower error values	1400:1417	lower error values (RMSE, MDA, and SSE)	1400:1438	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	12	30	theme	destructive	1683:1693	arg1	diseases					1711:1718	the destructive potato soft rot diseases	1679:1718	the destructive potato soft rot diseases	1679:1718	These results suggested its potential application for controlling the destructive potato soft rot diseases.
36528632	2	31	theme	novel	390:394	arg1	protocol					409:416	a novel biosynthesis protocol	388:416	a novel biosynthesis protocol for chitosan nanoparticles (CNPs)	388:450	The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp.
36528632	7	32	theme	 1	1040:1041	arg1	%					1042:1042	%	1042:1042	%	1042:1042	The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period.
36528632	0	33	theme	Innovative	0:9	arg1	biosynthesis					11:22	Innovative biosynthesis	0:22	Innovative biosynthesis	0:22	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	4	34	theme	morphological	639:651	arg1	properties					672:681	morphological, and physiological properties	639:681	morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964)	639:751	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	13	35	theme	CNPs	1769:1772	arg1	biosynthesis					1753:1764	the biosynthesis	1749:1764	the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence	1749:1936	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	11	36	theme	Streptomyces	1464:1475	arg1	strain					1489:1494	Streptomyces microflavus strain NEAE-83	1464:1502	Streptomyces microflavus strain NEAE-83	1464:1502	CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot.
36528632	6	37	theme	face-centered	898:910	arg1	CCFCD					938:942	CCFCD	938:942	CCFCD	938:942	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	6	37	theme	face-centered	898:910	arg1	design					930:935	face-centered central composite design	898:935	face-centered central composite design (CCFCD)	898:943	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	6	37	theme	face-centered	898:910	arg1	a mathematical model					876:895	a mathematical model	876:895	a mathematical model	876:895	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	0	38	theme	artificial	25:34	arg1	optimization					55:66	artificial intelligence-based optimization	25:66	artificial intelligence-based optimization	25:66	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	12	39	theme	rot	1707:1709	arg1	diseases					1711:1718	the destructive potato soft rot diseases	1679:1718	the destructive potato soft rot diseases	1679:1718	These results suggested its potential application for controlling the destructive potato soft rot diseases.
36528632	0	40	theme	inhibitory	154:163	arg1	potential					165:173	their inhibitory potential	148:173	their inhibitory potential against Pectobacterium carotovorum	148:208	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	11	41	theme	microflavus	1477:1487	arg1	strain					1489:1494	Streptomyces microflavus strain NEAE-83	1464:1502	Streptomyces microflavus strain NEAE-83	1464:1502	CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot.
36528632	1	42	theme	economic	261:268	arg1	advantages					299:308	economic, eco-friendly, and biosafety advantages	261:308	economic, eco-friendly, and biosafety advantages	261:308	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	13	43	dep	Streptomyces	1798:1809	arg1	microflavus					1811:1821	microflavus	1811:1821	microflavus	1811:1821	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	6	44	theme	central	912:918	arg1	CCFCD					938:942	CCFCD	938:942	CCFCD	938:942	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	6	44	theme	central	912:918	arg1	design					930:935	face-centered central composite design	898:935	face-centered central composite design (CCFCD)	898:943	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	6	44	theme	central	912:918	arg1	a mathematical model					876:895	a mathematical model	876:895	a mathematical model	876:895	CNPs biosynthesis was maximized using a mathematical model, face-centered central composite design (CCFCD).
36528632	13	45	theme	isolated	1788:1795	arg1	approach					1857:1864	an eco-friendly approach	1841:1864	an eco-friendly approach	1841:1864	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	13	45	theme	isolated	1788:1795	arg1	NEAE-83					1830:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	a newly isolated; Streptomyces microflavus strain NEAE-83	1780:1836	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	8	46	theme	CNPs	1181:1184	arg1	biosynthesis					1186:1197	CNPs biosynthesis	1181:1197	CNPs biosynthesis	1181:1197	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	7	47	theme	%	1042:1042	arg1	chitosan					1044:1051	 1% chitosan	1040:1051	 1% chitosan	1040:1051	The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period.
36528632	4	48	theme	accession	725:733	arg1	sequence					707:714	the 16S rRNA sequence	694:714	morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964)	639:751	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	4	48	theme	accession	725:733	arg1	number					735:740	GenBank accession number: MG384964	717:750	GenBank accession number: MG384964	717:750	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	4	49	theme	GenBank	717:723	arg1	sequence					707:714	the 16S rRNA sequence	694:714	morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964)	639:751	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	4	49	theme	GenBank	717:723	arg1	number					735:740	GenBank accession number: MG384964	717:750	GenBank accession number: MG384964	717:750	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	8	50	theme	trials	1212:1217	arg1	data					1219:1222	the trials data	1208:1222	the trials data of CCFCD	1208:1231	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	1	51	theme	eco-friendly	271:282	arg1	advantages					299:308	economic, eco-friendly, and biosafety advantages	261:308	economic, eco-friendly, and biosafety advantages	261:308	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	0	52	theme	intelligence-based	36:53	arg1	optimization					55:66	artificial intelligence-based optimization	25:66	artificial intelligence-based optimization	25:66	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	4	53	theme	physiological	658:670	arg1	properties					672:681	morphological, and physiological properties	639:681	morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964)	639:751	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	8	54	theme	neural	1120:1125	arg1	ANN					1136:1138	ANN	1136:1138	ANN	1136:1138	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	8	54	theme	neural	1120:1125	arg1	network					1127:1133	the artificial neural network	1105:1133	the artificial neural network (ANN)	1105:1139	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	8	54	theme	neural	1120:1125	arg1	Innovatively					1091:1102	Innovatively	1091:1102	Innovatively	1091:1102	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	10	55	dep	values	1412:1417	arg1	MDA					1426:1428	MDA	1426:1428	MDA	1426:1428	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	55	dep	values	1412:1417	arg1	SSE					1435:1437	SSE	1435:1437	SSE	1435:1437	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	55	dep	values	1412:1417	arg1	RMSE					1420:1423	RMSE	1420:1423	RMSE	1420:1423	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	55	dep	values	1412:1417	arg1	values					1412:1417	lower error values	1400:1417	lower error values (RMSE, MDA, and SSE)	1400:1438	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	11	56	theme	antibacterial	1520:1532	arg1	activity					1534:1541	in-vitro antibacterial activity	1511:1541	in-vitro antibacterial activity	1511:1541	CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot.
36528632	2	57	theme	Streptomyces	473:484	arg1	sp					486:487	a pioneer Streptomyces sp	463:487	a pioneer Streptomyces sp	463:487	The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp.
36528632	4	58	theme	microflavus	603:613	arg1	It					569:570	It	569:570	It	569:570	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	4	58	theme	microflavus	603:613	arg1	strain					615:620	Streptomyces microflavus strain NEAE-83	590:628	Streptomyces microflavus strain NEAE-83	590:628	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	7	59	theme	an initial	1019:1028	arg1	pH of					1030:1034	an initial pH of 5.5	1019:1038	an initial pH of 5.5	1019:1038	The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period.
36528632	2	60	theme	chitosan	422:429	arg1	CNPs					446:449	CNPs	446:449	CNPs	446:449	The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp.
36528632	2	60	theme	chitosan	422:429	arg1	nanoparticles					431:443	chitosan nanoparticles	422:443	chitosan nanoparticles (CNPs)	422:450	The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp.
36528632	0	61	theme	Pectobacterium	183:196	arg1	carotovorum					198:208	Pectobacterium carotovorum	183:208	Pectobacterium carotovorum	183:208	Innovative biosynthesis, artificial intelligence-based optimization, and characterization of chitosan nanoparticles by Streptomyces microflavus and their inhibitory potential against Pectobacterium carotovorum.
36528632	13	62	from	report	1739:1744	arg1	biosynthesis					1753:1764	the biosynthesis	1749:1764	the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence	1749:1936	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	10	63	theme	error	1406:1410	arg1	MDA					1426:1428	MDA	1426:1428	MDA	1426:1428	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	63	theme	error	1406:1410	arg1	SSE					1435:1437	SSE	1435:1437	SSE	1435:1437	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	63	theme	error	1406:1410	arg1	RMSE					1420:1423	RMSE	1420:1423	RMSE	1420:1423	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	10	63	theme	error	1406:1410	arg1	values					1412:1417	lower error values	1400:1417	lower error values (RMSE, MDA, and SSE)	1400:1438	ANN had a higher prediction efficacy and, lower error values (RMSE, MDA, and SSE).
36528632	13	64	theme	artificial	1914:1923	arg1	intelligence					1925:1936	artificial intelligence	1914:1936	artificial intelligence	1914:1936	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	9	65	theme	CNPs	1321:1324	arg1	biosynthesis					1326:1337	CNPs biosynthesis	1321:1337	CNPs biosynthesis	1321:1337	Despite the high precision degree of both models, ANN was supreme in the prediction of CNPs biosynthesis compared to CCFCD.
36528632	8	66	dep	used for	1146:1153	arg1	validating					1155:1164	validating	1155:1164	validating	1155:1164	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	8	66	dep	used for	1146:1153	arg1	predicting					1170:1179	predicting	1170:1179	predicting CNPs biosynthesis based on the trials data of CCFCD	1170:1231	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	12	67	theme	potato	1695:1700	arg1	diseases					1711:1718	the destructive potato soft rot diseases	1679:1718	the destructive potato soft rot diseases	1679:1718	These results suggested its potential application for controlling the destructive potato soft rot diseases.
36528632	11	68	theme	in-vitro	1511:1518	arg1	activity					1534:1541	in-vitro antibacterial activity	1511:1541	in-vitro antibacterial activity	1511:1541	CNPs biosynthesized by Streptomyces microflavus strain NEAE-83 showed in-vitro antibacterial activity against Pectobacterium carotovorum, which causes the potato soft rot.
36528632	3	69	theme	CNPs	550:553	arg1	biosynthesis					555:566	CNPs biosynthesis	550:566	CNPs biosynthesis	550:566	strain NEAE-83, which exhibited a significant potential for CNPs biosynthesis.
36528632	7	70	theme	highest	950:956	arg1	yield					958:962	The highest yield	946:962	The highest yield of CNPs (9.41 mg/mL)	946:983	The highest yield of CNPs (9.41 mg/mL) was obtained in run no. 27, using an initial pH of 5.5, 1% chitosan, 40 °C, and a 12 h incubation period.
36528632	9	71	theme	biosynthesis	1326:1337	arg1	prediction					1307:1316	the prediction	1303:1316	the prediction of CNPs biosynthesis	1303:1337	Despite the high precision degree of both models, ANN was supreme in the prediction of CNPs biosynthesis compared to CCFCD.
36528632	13	72	dep	isolated	1788:1795	arg1	Streptomyces					1798:1809	Streptomyces	1798:1809	Streptomyces	1798:1809	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	2	73	theme	biosynthesis	396:407	arg1	protocol					409:416	a novel biosynthesis protocol	388:416	a novel biosynthesis protocol for chitosan nanoparticles (CNPs)	388:450	The current study describes a novel biosynthesis protocol for chitosan nanoparticles (CNPs), employing a pioneer Streptomyces sp.
36528632	1	74	theme	biosafety	289:297	arg1	advantages					299:308	economic, eco-friendly, and biosafety advantages	261:308	economic, eco-friendly, and biosafety advantages	261:308	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	8	75	theme	artificial	1109:1118	arg1	ANN					1136:1138	ANN	1136:1138	ANN	1136:1138	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	8	75	theme	artificial	1109:1118	arg1	network					1127:1133	the artificial neural network	1105:1133	the artificial neural network (ANN)	1105:1139	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	8	75	theme	artificial	1109:1118	arg1	Innovatively					1091:1102	Innovatively	1091:1102	Innovatively	1091:1102	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	3	76	theme	significant	524:534	arg1	potential					536:544	a significant potential	522:544	a significant potential for CNPs biosynthesis	522:566	strain NEAE-83, which exhibited a significant potential for CNPs biosynthesis.
36528632	8	77	theme	CCFCD	1227:1231	arg1	data					1219:1222	the trials data	1208:1222	the trials data of CCFCD	1208:1231	Innovatively, the artificial neural network (ANN), was used for validating and predicting CNPs biosynthesis based on the trials data of CCFCD.
36528632	4	78	theme	rRNA	702:705	arg1	sequence					707:714	the 16S rRNA sequence	694:714	morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964)	639:751	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	4	78	theme	rRNA	702:705	arg1	number					735:740	GenBank accession number: MG384964	717:750	GenBank accession number: MG384964	717:750	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
36528632	9	79	theme	precision	1251:1259	arg1	degree					1261:1266	the high precision degree	1242:1266	the high precision degree of both models	1242:1281	Despite the high precision degree of both models, ANN was supreme in the prediction of CNPs biosynthesis compared to CCFCD.
36528632	13	80	theme	biosynthesis	1890:1901	arg1	process					1903:1909	the biosynthesis process	1886:1909	the biosynthesis process	1886:1909	This is the first report on the biosynthesis of CNPs using a newly isolated; Streptomyces microflavus strain NEAE-83 as an eco-friendly approach and optimization of the biosynthesis process by artificial intelligence.
36528632	1	81	from	strategy	227:234	arg1	nanotechnology					239:252	nanotechnology	239:252	nanotechnology	239:252	Microbial-based strategy in nanotechnology offers economic, eco-friendly, and biosafety advantages over traditional chemical and physical protocols.
36528632	4	82	theme	16S	698:700	arg1	rRNA					702:705	16S rRNA	698:705	morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964)	639:751	It was identified as Streptomyces microflavus strain NEAE-83 based on morphological, and physiological properties as well as the 16S rRNA sequence (GenBank accession number: MG384964).
35609771	9	0	theme	therapeutic	1772:1782	arg1	CSR					1749:1751	CSR	1749:1751	CSR	1749:1751	In conclusion, this study provides the strong evidence that CSR may be a promising therapeutic drug for UC.
35609771	9	0	theme	therapeutic	1772:1782	arg1	drug					1784:1787	a promising therapeutic drug	1760:1787	a promising therapeutic drug for UC	1760:1794	In conclusion, this study provides the strong evidence that CSR may be a promising therapeutic drug for UC.
35609771	5	1	theme	intestinal	1097:1106	arg1	permeability					1108:1119	intestinal permeability	1097:1119	intestinal permeability	1097:1119	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	1	2	theme	microbiota	242:251	arg1	dysbiosis					253:261	microbiota dysbiosis	242:261	microbiota dysbiosis	242:261	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	0	3	from	Effects	11:17	arg1	Balance					42:48	Immune Balance	35:48	Immune Balance	35:48	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	2	4	theme	immune-inhibitory	350:366	arg1	effects					368:374	strong anti-inflammatory and immune-inhibitory effects	321:374	strong anti-inflammatory and immune-inhibitory effects	321:374	Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects.
35609771	0	5	theme	Ulcerative	95:104	arg1	Colitis					106:112	Experimental Ulcerative Colitis	82:112	Experimental Ulcerative Colitis Mice	82:117	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	8	6	theme	gut	1514:1516	arg1	diversity					1529:1537	the gut microbiota diversity	1510:1537	the gut microbiota diversity	1510:1537	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	6	7	theme	pro-inflammatory	1170:1185	arg1	cytokines					1187:1195	pro-inflammatory cytokines	1170:1195	pro-inflammatory cytokines	1170:1195	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	8	8	theme	microbiota-mediated	1649:1667	arg1	effects					1680:1686	the gut microbiota-mediated protective effects	1641:1686	the gut microbiota-mediated protective effects	1641:1686	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	0	9	theme	Experimental	82:93	arg1	Colitis					106:112	Experimental Ulcerative Colitis	82:112	Experimental Ulcerative Colitis Mice	82:117	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	6	10	theme	protein	1273:1279	arg1	levels					1281:1286	both mRNA and protein levels	1259:1286	both mRNA and protein levels	1259:1286	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	8	11	theme	gut	1645:1647	arg1	effects					1680:1686	the gut microbiota-mediated protective effects	1641:1686	the gut microbiota-mediated protective effects	1641:1686	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	4	12	theme	microbiota	758:767	arg1	transplantation					769:783	fecal microbiota transplantation	752:783	fecal microbiota transplantation (FMT)	752:789	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	4	12	theme	microbiota	758:767	arg1	FMT					786:788	FMT	786:788	FMT	786:788	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	4	13	theme	therapeutic	590:600	arg1	effects					602:608	the therapeutic effects	586:608	the therapeutic effects	586:608	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	6	14	theme	cytokines	1187:1195	arg1	production					1156:1165	the production	1152:1165	the production of pro-inflammatory cytokines	1152:1195	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	4	15	theme	treating	648:655	arg1	UC					657:658	treating UC	648:658	treating UC	648:658	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	5	16	theme	CSR	864:866	arg1	administration					868:881	CSR administration	864:881	CSR administration	864:881	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	3	17	theme	colonic	421:427	arg1	inflammation					429:440	colonic inflammation	421:440	colonic inflammation	421:440	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	1	18	theme	chronic	149:155	arg1	colitis					131:137	Ulcerative colitis	120:137	Ulcerative colitis (UC)	120:142	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	1	18	theme	chronic	149:155	arg1	disease					176:182	a chronic inflammatory bowel disease	147:182	a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis	147:261	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	0	19	theme	Colitis	106:112	arg1	Mice					114:117	Experimental Ulcerative Colitis Mice	82:117	Experimental Ulcerative Colitis Mice	82:117	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	7	20	theme	gut	1440:1442	arg1	manner					1465:1470	a gut microbiota-dependent manner	1438:1470	a gut microbiota-dependent manner	1438:1470	Notably, all the therapeutic effects were exerted in a gut microbiota-dependent manner.
35609771	3	21	theme	CSR	414:416	arg1	effects					403:409	the effects	399:409	the effects of CSR on colonic inflammation and mucosal immunity	399:461	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	1	22	theme	inflammatory	157:168	arg1	colitis					131:137	Ulcerative colitis	120:137	Ulcerative colitis (UC)	120:142	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	1	22	theme	inflammatory	157:168	arg1	disease					176:182	a chronic inflammatory bowel disease	147:182	a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis	147:261	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	7	23	theme	microbiota-dependent	1444:1463	arg1	manner					1465:1470	a gut microbiota-dependent manner	1438:1470	a gut microbiota-dependent manner	1438:1470	Notably, all the therapeutic effects were exerted in a gut microbiota-dependent manner.
35609771	4	24	from	mechanism	628:636	arg1	UC					657:658	treating UC	648:658	treating UC	648:658	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	5	25	theme	-induced	941:948	arg1	colitis					950:956	the dextran sodium sulfate (DSS)-induced colitis	909:956	the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability	909:1119	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	1	26	theme	bowel	170:174	arg1	colitis					131:137	Ulcerative colitis	120:137	Ulcerative colitis (UC)	120:142	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	1	26	theme	bowel	170:174	arg1	disease					176:182	a chronic inflammatory bowel disease	147:182	a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis	147:261	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	0	27	theme	Beneficial	0:9	arg1	Effects					11:17	Beneficial Effects	0:17	Beneficial Effects of Celastrol on Immune Balance	0:48	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	4	28	theme	cell	842:845	arg1	differentiation					847:861	cell differentiation	842:861	cell differentiation	842:861	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	8	29	theme	microbiota	1579:1588	arg1	compositions					1555:1566	the compositions	1551:1566	the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects	1551:1686	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	3	30	from	effects	403:409	arg1	inflammation					429:440	colonic inflammation	421:440	colonic inflammation	421:440	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	3	30	from	effects	403:409	arg1	immunity					454:461	mucosal immunity	446:461	mucosal immunity	446:461	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	4	31	theme	histological	666:677	arg1	staining					679:686	histological staining	666:686	histological staining	666:686	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	0	32	theme	Celastrol	22:30	arg1	Effects					11:17	Beneficial Effects	0:17	Beneficial Effects of Celastrol on Immune Balance	0:48	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	5	33	theme	recovered	994:1002	arg1	weight					1009:1014	recovered body weight	994:1014	recovered body weight	994:1014	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	5	34	theme	length	1026:1031	arg1	score					1087:1091	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	3	35	theme	protective	549:558	arg1	effects					560:566	the protective effects	545:566	the protective effects	545:566	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	6	36	theme	mediators	1246:1254	arg1	amount					1218:1223	the amount	1214:1223	the amount of anti-inflammatory mediators	1214:1254	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	6	36	theme	mediators	1246:1254	arg1	mediators					1246:1254	anti-inflammatory mediators	1228:1254	anti-inflammatory mediators	1228:1254	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	4	37	theme	CSR	641:643	arg1	effects					602:608	the therapeutic effects	586:608	the therapeutic effects	586:608	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	4	37	theme	CSR	641:643	arg1	mechanism					628:636	the potential mechanism	614:636	the potential mechanism	614:636	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	4	38	theme	flow	736:739	arg1	cytometry					741:749	flow cytometry	736:749	flow cytometry	736:749	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	8	39	theme	gut	1575:1577	arg1	microbiota					1579:1588	gut microbiota	1575:1588	gut microbiota	1575:1588	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	6	40	theme	Treg/Th17	1331:1339	arg1	balances					1306:1313	the balances	1302:1313	the balances of Treg/Th1 and Treg/Th17	1302:1339	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	5	41	theme	decreased	1048:1056	arg1	DAI					1082:1084	DAI	1082:1084	DAI	1082:1084	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	5	41	theme	decreased	1048:1056	arg1	index					1075:1079	the decreased disease activity index	1044:1079	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	4	42	theme	permeability	700:711	arg1	assay					713:717	intestinal permeability assay	689:717	intestinal permeability assay	689:717	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	3	43	theme	experimental	469:480	arg1	model					490:494	an experimental colitis model	466:494	an experimental colitis model	466:494	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	0	44	theme	Immune	35:40	arg1	Balance					42:48	Immune Balance	35:48	Immune Balance	35:48	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	2	45	theme	anti-inflammatory	328:344	arg1	effects					368:374	strong anti-inflammatory and immune-inhibitory effects	321:374	strong anti-inflammatory and immune-inhibitory effects	321:374	Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects.
35609771	9	46	theme	strong	1728:1733	arg1	evidence					1735:1742	the strong evidence that CSR may be a promising therapeutic drug for UC	1724:1794	the strong evidence that CSR may be a promising therapeutic drug for UC	1724:1794	In conclusion, this study provides the strong evidence that CSR may be a promising therapeutic drug for UC.
35609771	0	47	from	Microbiota	68:77	arg1	Mice					114:117	Experimental Ulcerative Colitis Mice	82:117	Experimental Ulcerative Colitis Mice	82:117	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	5	48	theme	weight	1009:1014	arg1	score					1087:1091	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	5	49	from	colitis	950:956	arg1	mice					961:964	mice	961:964	mice	961:964	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	1	50	theme	many	194:197	arg1	inflammation					225:236	colonic inflammation	217:236	colonic inflammation	217:236	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	1	50	theme	many	194:197	arg1	dysbiosis					253:261	microbiota dysbiosis	242:261	microbiota dysbiosis	242:261	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	1	50	theme	many	194:197	arg1	factors					199:205	many factors	194:205	many factors including colonic inflammation and microbiota dysbiosis	194:261	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	6	51	theme	mRNA	1264:1267	arg1	levels					1281:1286	both mRNA and protein levels	1259:1286	both mRNA and protein levels	1259:1286	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	6	52	theme	Treg/Th1	1318:1325	arg1	balances					1306:1313	the balances	1302:1313	the balances of Treg/Th1 and Treg/Th17	1302:1339	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	5	53	theme	activity	1066:1073	arg1	DAI					1082:1084	DAI	1082:1084	DAI	1082:1084	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	5	53	theme	activity	1066:1073	arg1	index					1075:1079	the decreased disease activity index	1044:1079	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	6	54	theme	anti-inflammatory	1228:1244	arg1	mediators					1246:1254	anti-inflammatory mediators	1228:1254	anti-inflammatory mediators	1228:1254	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	8	55	dep	microbiota	1579:1588	arg1	the					1571:1573	the	1571:1573	the	1571:1573	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	8	56	theme	metabolites	1594:1604	arg1	compositions					1555:1566	the compositions	1551:1566	the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects	1551:1686	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	4	57	from	effects	602:608	arg1	UC					657:658	treating UC	648:658	treating UC	648:658	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	6	58	theme	immune	1365:1370	arg1	homeostasis					1372:1382	the colonic immune homeostasis	1353:1382	the colonic immune homeostasis	1353:1382	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	5	59	theme	index	1075:1079	arg1	score					1087:1091	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	5	60	theme	colon	1020:1024	arg1	length					1026:1031	colon length	1020:1031	colon length	1020:1031	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	4	61	theme	cytokine	720:727	arg1	assay					729:733	cytokine assay	720:733	cytokine assay	720:733	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	7	62	theme	therapeutic	1402:1412	arg1	effects					1414:1420	all the therapeutic effects	1394:1420	all the therapeutic effects	1394:1420	Notably, all the therapeutic effects were exerted in a gut microbiota-dependent manner.
35609771	3	63	theme	colitis	482:488	arg1	model					490:494	an experimental colitis model	466:494	an experimental colitis model	466:494	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	4	64	theme	untargeted	813:822	arg1	metabolomics					824:835	untargeted metabolomics	813:835	untargeted metabolomics	813:835	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	1	65	theme	Ulcerative	120:129	arg1	colitis					131:137	Ulcerative colitis	120:137	Ulcerative colitis (UC)	120:142	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	1	65	theme	Ulcerative	120:129	arg1	disease					176:182	a chronic inflammatory bowel disease	147:182	a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis	147:261	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	1	65	theme	Ulcerative	120:129	arg1	UC					140:141	UC	140:141	UC	140:141	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	5	66	theme	body	1004:1007	arg1	weight					1009:1014	recovered body weight	994:1014	recovered body weight	994:1014	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	8	67	theme	protective	1669:1678	arg1	effects					1680:1686	the gut microbiota-mediated protective effects	1641:1686	the gut microbiota-mediated protective effects	1641:1686	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	2	68	theme	Previous	264:271	arg1	studies					273:279	Previous studies	264:279	Previous studies	264:279	Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects.
35609771	2	69	theme	strong	321:326	arg1	effects					368:374	strong anti-inflammatory and immune-inhibitory effects	321:374	strong anti-inflammatory and immune-inhibitory effects	321:374	Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects.
35609771	4	70	theme	fecal	752:756	arg1	transplantation					769:783	fecal microbiota transplantation	752:783	fecal microbiota transplantation (FMT)	752:789	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	4	70	theme	fecal	752:756	arg1	FMT					786:788	FMT	786:788	FMT	786:788	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	5	71	theme	disease	1058:1064	arg1	DAI					1082:1084	DAI	1082:1084	DAI	1082:1084	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	5	71	theme	disease	1058:1064	arg1	index					1075:1079	the decreased disease activity index	1044:1079	the recovered body weight and colon length as well as the decreased disease activity index (DAI) score	990:1091	CSR administration significantly ameliorated the dextran sodium sulfate (DSS)-induced colitis in mice, which was evidenced by the recovered body weight and colon length as well as the decreased disease activity index (DAI) score and intestinal permeability.
35609771	6	72	theme	colonic	1357:1363	arg1	homeostasis					1372:1382	the colonic immune homeostasis	1353:1382	the colonic immune homeostasis	1353:1382	Meanwhile, CSR down-regulated the production of pro-inflammatory cytokines and up-regulated the amount of anti-inflammatory mediators at both mRNA and protein levels, and improved the balances of Treg/Th1 and Treg/Th17 to maintain the colonic immune homeostasis.
35609771	1	73	theme	colonic	217:223	arg1	inflammation					225:236	colonic inflammation	217:236	colonic inflammation	217:236	Ulcerative colitis (UC) is a chronic inflammatory bowel disease caused by many factors including colonic inflammation and microbiota dysbiosis.
35609771	0	74	theme	Gut	64:66	arg1	Microbiota					68:77	Gut Microbiota	64:77	Gut Microbiota in Experimental Ulcerative Colitis Mice	64:117	Beneficial Effects of Celastrol on Immune Balance by Modulating Gut Microbiota in Experimental Ulcerative Colitis Mice.
35609771	8	75	theme	CSR	1486:1488	arg1	treatment					1490:1498	CSR treatment	1486:1498	CSR treatment	1486:1498	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	4	76	theme	rRNA	796:799	arg1	sequencing					801:810	16S rRNA sequencing	792:810	16S rRNA sequencing	792:810	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	4	77	theme	intestinal	689:698	arg1	assay					713:717	intestinal permeability assay	689:717	intestinal permeability assay	689:717	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	3	78	theme	mucosal	446:452	arg1	immunity					454:461	mucosal immunity	446:461	mucosal immunity	446:461	Here, we investigated the effects of CSR on colonic inflammation and mucosal immunity in an experimental colitis model, and addressed the mechanism by which CSR exerts the protective effects.
35609771	9	79	theme	promising	1762:1770	arg1	CSR					1749:1751	CSR	1749:1751	CSR	1749:1751	In conclusion, this study provides the strong evidence that CSR may be a promising therapeutic drug for UC.
35609771	9	79	theme	promising	1762:1770	arg1	drug					1784:1787	a promising therapeutic drug	1760:1787	a promising therapeutic drug for UC	1760:1794	In conclusion, this study provides the strong evidence that CSR may be a promising therapeutic drug for UC.
35609771	2	80	contain	has	317:319	arg2	effects					368:374	strong anti-inflammatory and immune-inhibitory effects	321:374	strong anti-inflammatory and immune-inhibitory effects	321:374	Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects.
35609771	2	80	contain	has	317:319	arg1	CSR					312:314	CSR	312:314	CSR	312:314	Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects.
35609771	2	80	contain	has	317:319	arg1	celastrol					301:309	celastrol	301:309	celastrol (CSR)	301:315	Previous studies have indicated that celastrol (CSR) has strong anti-inflammatory and immune-inhibitory effects.
35609771	4	81	theme	16S	792:794	arg1	sequencing					801:810	16S rRNA sequencing	792:810	16S rRNA sequencing	792:810	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
35609771	8	82	theme	microbiota	1518:1527	arg1	diversity					1529:1537	the gut microbiota diversity	1510:1537	the gut microbiota diversity	1510:1537	Furthermore, CSR treatment increased the gut microbiota diversity and changed the compositions of the gut microbiota and metabolites, which is probably associated with the gut microbiota-mediated protective effects.
35609771	4	83	theme	potential	618:626	arg1	mechanism					628:636	the potential mechanism	614:636	the potential mechanism	614:636	We characterized the therapeutic effects and the potential mechanism of CSR on treating UC using histological staining, intestinal permeability assay, cytokine assay, flow cytometry, fecal microbiota transplantation (FMT), 16S rRNA sequencing, untargeted metabolomics, and cell differentiation.
37256902	7	0	dep	decreased	1305:1313	arg1	<					1318:1318	p < 0.05	1316:1323	p < 0.05	1316:1323	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	1	1	from	comparison	911:920	arg1	g					234:234	g	234:234	g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C)	234:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	2	theme	substrate	331:339	arg1	g					322:322	100 g	318:322	100 g of dry substrate	318:339	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	3	from	/bag	255:258	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	4	from	Pe-PQ	528:532	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	5	theme	substrate	159:167	arg1	digestibility					129:141	ruminal in vitro digestibility	112:141	ruminal in vitro digestibility	112:141	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	0	5	theme	substrate	159:167	arg1	composition					96:106	chemical composition	87:106	chemical composition	87:106	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	8	6	dep	edodes	1501:1506	arg1	L15					1513:1515	L15	1513:1515	L15	1513:1515	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	6	dep	edodes	1501:1506	arg1	L9					1522:1523	L9	1522:1523	L9	1522:1523	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	6	dep	edodes	1501:1506	arg1	L5					1509:1510	L5	1509:1510	L5	1509:1510	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	6	dep	edodes	1501:1506	arg1	edodes					1501:1506	Lentinula edodes	1491:1506	Lentinula edodes (L5, L15, and L9)	1491:1524	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	2	7	theme	randomized	1003:1012	arg1	variables					963:971	The variables	959:971	The variables	959:971	The variables were analyzed as a completely randomized block design using R 4.0.3 software.
37256902	2	7	theme	randomized	1003:1012	arg1	design					1020:1025	a completely randomized block design	990:1025	a completely randomized block design using R 4.0.3 software	990:1048	The variables were analyzed as a completely randomized block design using R 4.0.3 software.
37256902	1	8	theme	spent	876:880	arg1	substrate					845:853	the residual substrate	832:853	the residual substrate of CS	832:859	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	8	theme	spent	876:880	arg1	SMS					902:904	SMS	902:904	SMS	902:904	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	8	theme	spent	876:880	arg1	substrate					891:899	the so called spent mushroom substrate	862:899	the so called spent mushroom substrate (SMS)	862:905	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	9	theme	lignin	680:685	arg1	content					649:655	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	10	theme	fungal	348:353	arg1	Po-P14					407:412	Po-P14	407:412	Po-P14	407:412	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	10	theme	fungal	348:353	arg1	Po-Psma					441:447	Po-Psma	441:447	Po-Psma	441:447	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	10	theme	fungal	348:353	arg1	Po-IAP					391:396	Pleurotus ostreatus Po-IAP	371:396	Pleurotus ostreatus Po-IAP	371:396	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	10	theme	fungal	348:353	arg1	Pd-PRO					480:485	Pd-PRO	480:485	Pd-PRO	480:485	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	10	theme	fungal	348:353	arg1	Po-IE202					415:422	Po-IE202	415:422	Po-IE202	415:422	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	10	theme	fungal	348:353	arg1	strains					355:361	17 fungal strains	345:361	17 fungal strains	345:361	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	10	theme	fungal	348:353	arg1	Po-P38					399:404	Po-P38	399:404	Po-P38	399:404	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	8	11	theme	nutritional	1650:1660	arg1	improvement					1662:1672	its nutritional improvement	1646:1672	its nutritional improvement	1646:1672	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	12	from	improvement	1662:1672	arg1	IVDMD					1690:1694	IVDMD	1690:1694	IVDMD	1690:1694	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	7	13	theme	p	1316:1316	arg1	<					1318:1318	p < 0.05	1316:1323	p < 0.05	1316:1323	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	1	14	theme	Pe-MB	518:522	arg1	g					234:234	g	234:234	g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C)	234:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	15	theme	chemical	87:94	arg1	composition					96:106	chemical composition	87:106	chemical composition	87:106	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	6	16	theme	Hericium	1155:1162	arg1	spp.					1164:1167	Hericium spp.	1155:1167	Hericium spp.	1155:1167	and Hericium spp., presented better BE and P.
37256902	0	17	from	stover	58:63	arg1	digestibility					129:141	ruminal in vitro digestibility	112:141	ruminal in vitro digestibility	112:141	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	0	17	from	stover	58:63	arg1	composition					96:106	chemical composition	87:106	chemical composition	87:106	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	18	theme	mushrooms	304:312	arg1	g					293:293	g	293:293	g of fresh mushrooms per 100 g of dry substrate	293:339	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	8	19	from	increase	1678:1685	arg1	IVDMD					1690:1694	IVDMD	1690:1694	IVDMD	1690:1694	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	7	20	theme	CP	1239:1240	arg1	content					1242:1248	the CP content	1235:1248	the CP content (p < 0.05)	1235:1259	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	7	20	theme	CP	1239:1240	arg1	<					1253:1253	p < 0.05	1251:1258	p < 0.05	1251:1258	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	1	21	theme	Lentinula	535:543	arg1	L15					552:554	Lentinula edodes L15	535:554	Lentinula edodes L15	535:554	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	22	theme	in	120:121	arg1	digestibility					129:141	ruminal in vitro digestibility	112:141	ruminal in vitro digestibility	112:141	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	23	theme	crude	660:664	arg1	protein					666:672	crude protein	660:672	crude protein (CP)	660:677	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	23	theme	crude	660:664	arg1	CP					675:676	CP	675:676	CP	675:676	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	10	24	theme	fruiting	1825:1832	arg1	bodies					1834:1839	fruiting bodies	1825:1839	fruiting bodies	1825:1839	fruiting bodies, with strain Po-IAP as the best yielding.
37256902	0	25	dep	in	120:121	arg1	vitro					123:127	vitro	123:127	vitro	123:127	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	26	dep	production	218:227	arg1	g					234:234	g	234:234	g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C)	234:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	8	27	theme	Lentinula	1491:1499	arg1	L15					1513:1515	L15	1513:1515	L15	1513:1515	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	27	theme	Lentinula	1491:1499	arg1	L9					1522:1523	L9	1522:1523	L9	1522:1523	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	27	theme	Lentinula	1491:1499	arg1	L5					1509:1510	L5	1509:1510	L5	1509:1510	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	27	theme	Lentinula	1491:1499	arg1	edodes					1501:1506	Lentinula edodes	1491:1506	Lentinula edodes (L5, L15, and L9)	1491:1524	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	1	28	from	Heri	596:599	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	10	29	theme	strain	1847:1852	arg1	Po-IAP					1854:1859	strain Po-IAP	1847:1859	strain Po-IAP as the best yielding	1847:1880	fruiting bodies, with strain Po-IAP as the best yielding.
37256902	7	30	theme	p	1382:1382	arg1	<					1384:1384	p < 0.05	1382:1389	p < 0.05	1382:1389	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	1	31	theme	efficiency	276:285	arg1	Pe-MB					518:522	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB	265:522	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB	265:522	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	32	dep	Pe-MB	518:522	arg1	assess					638:643	assess	638:643	to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	635:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	32	dep	Pe-MB	518:522	arg1	produced					602:609	produced	602:609	produced in corn stover (CS)	602:629	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	33	theme	non-inoculated	929:942	arg1	C					955:955	C	955:955	C	955:955	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	33	theme	non-inoculated	929:942	arg1	substrate					944:952	the non-inoculated substrate	925:952	the non-inoculated substrate (C)	925:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	34	theme	Pd-UTMR	491:497	arg1	Pe-MB					518:522	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB	265:522	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB	265:522	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	35	dep	Pleurotus	500:508	arg1	eryngii					510:516	Pleurotus eryngii	500:516	Pleurotus eryngii	500:516	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	L5					561:562	L5	561:562	L5	561:562	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	/bag					255:258	fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C)	239:956	/bag	255:258	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	Heri					596:599	Hericium erinaceus Heri	577:599	Hericium erinaceus Heri	577:599	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	Pe-MB					518:522	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB	265:522	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB	265:522	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	L15					552:554	Lentinula edodes L15	535:554	Lentinula edodes L15	535:554	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	LC					569:570	LC	569:570	LC	569:570	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	L9					557:558	L9	557:558	L9	557:558	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	digestibility					793:805	in vitro digestibility	784:805	in vitro digestibility	784:805	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	36	theme	matter	814:819	arg1	Pe-PQ					528:532	Pe-PQ	528:532	Pe-PQ	528:532	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	10	37	theme	best	1868:1871	arg1	yielding					1873:1880	the best yielding	1864:1880	the best yielding	1864:1880	fruiting bodies, with strain Po-IAP as the best yielding.
37256902	1	38	dep	Pleurotus	371:379	arg1	ostreatus					381:389	ostreatus	381:389	ostreatus	381:389	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	39	from	Pe-MB	518:522	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	40	theme	neutral	750:756	arg1	fiber					768:772	neutral detergent fiber	750:772	neutral detergent fiber (NDF)	750:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	40	theme	neutral	750:756	arg1	NDF					775:777	NDF	775:777	NDF	775:777	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	2	41	theme	4.0.3	1035:1039	arg1	software					1041:1048	R 4.0.3 software	1033:1048	R 4.0.3 software	1033:1048	The variables were analyzed as a completely randomized block design using R 4.0.3 software.
37256902	1	42	theme	fiber	768:772	arg1	content					649:655	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	43	from	digestibility	793:805	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	44	theme	fresh	239:243	arg1	mushrooms					245:253	fresh mushrooms	239:253	fresh mushrooms	239:253	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	8	45	theme	edodes	1501:1506	arg1	strain					1578:1583	one strain	1574:1583	one strain of Pleurotus ostreatus (Po-IAP)	1574:1615	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	45	theme	edodes	1501:1506	arg1	strains					1480:1486	the 3 strains	1474:1486	the 3 strains of Lentinula edodes (L5, L15, and L9)	1474:1524	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	45	theme	edodes	1501:1506	arg1	strain					1531:1536	one strain	1527:1536	one strain of Pleurotus djamour (Pd-UTMR)	1527:1567	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	1	46	theme	erinaceus	586:594	arg1	Heri					596:599	Hericium erinaceus Heri	577:599	Hericium erinaceus Heri	577:599	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	7	47	dep	increased	1371:1379	arg1	<					1384:1384	p < 0.05	1382:1389	p < 0.05	1382:1389	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	0	48	theme	mushroom	35:42	arg1	strains					17:23	17 strains	14:23	17 strains of edible mushroom	14:42	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	49	theme	/bag	255:258	arg1	g					234:234	g	234:234	g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C)	234:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	50	theme	hemicellulose	703:715	arg1	content					649:655	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	51	theme	study	192:196	arg1	objective					174:182	The objective	170:182	The objective of this study	170:196	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	52	theme	acid	718:721	arg1	fiber					733:737	acid detergent fiber	718:737	acid detergent fiber (ADF)	718:743	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	52	theme	acid	718:721	arg1	ADF					740:742	ADF	740:742	ADF	740:742	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	53	theme	edodes	545:550	arg1	L15					552:554	Lentinula edodes L15	535:554	Lentinula edodes L15	535:554	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	54	theme	CS	858:859	arg1	substrate					845:853	the residual substrate	832:853	the residual substrate of CS	832:859	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	54	theme	CS	858:859	arg1	substrate					891:899	the so called spent mushroom substrate	862:899	the so called spent mushroom substrate (SMS)	862:905	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	55	theme	fiber	733:737	arg1	content					649:655	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	56	theme	residual	150:157	arg1	substrate					159:167	the residual substrate	146:167	the residual substrate	146:167	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	2	57	theme	block	1014:1018	arg1	variables					963:971	The variables	959:971	The variables	959:971	The variables were analyzed as a completely randomized block design using R 4.0.3 software.
37256902	2	57	theme	block	1014:1018	arg1	design					1020:1025	a completely randomized block design	990:1025	a completely randomized block design using R 4.0.3 software	990:1048	The variables were analyzed as a completely randomized block design using R 4.0.3 software.
37256902	1	58	theme	called	869:874	arg1	substrate					845:853	the residual substrate	832:853	the residual substrate of CS	832:859	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	58	theme	called	869:874	arg1	SMS					902:904	SMS	902:904	SMS	902:904	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	58	theme	called	869:874	arg1	substrate					891:899	the so called spent mushroom substrate	862:899	the so called spent mushroom substrate (SMS)	862:905	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	59	from	g	234:234	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	60	theme	mushroom	882:889	arg1	substrate					845:853	the residual substrate	832:853	the residual substrate of CS	832:859	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	60	theme	mushroom	882:889	arg1	SMS					902:904	SMS	902:904	SMS	902:904	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	60	theme	mushroom	882:889	arg1	substrate					891:899	the so called spent mushroom substrate	862:899	the so called spent mushroom substrate (SMS)	862:905	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	6	61	dep	better	1180:1185	arg1	BE					1187:1188	BE	1187:1188	BE	1187:1188	and Hericium spp., presented better BE and P.
37256902	1	62	theme	strains	355:361	arg1	efficiency					276:285	biological efficiency	265:285	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO	265:485	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	62	theme	strains	355:361	arg1	Pleurotus					500:508	Pleurotus	500:508	Pleurotus	500:508	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	62	theme	strains	355:361	arg1	Pd-UTMR					491:497	Pd-UTMR	491:497	Pd-UTMR	491:497	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	7	63	theme	p	1251:1251	arg1	content					1242:1248	the CP content	1235:1248	the CP content (p < 0.05)	1235:1259	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	7	63	theme	p	1251:1251	arg1	<					1253:1253	p < 0.05	1251:1258	p < 0.05	1251:1258	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	8	64	theme	djamour	1551:1557	arg1	strain					1578:1583	one strain	1574:1583	one strain of Pleurotus ostreatus (Po-IAP)	1574:1615	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	64	theme	djamour	1551:1557	arg1	strains					1480:1486	the 3 strains	1474:1486	the 3 strains of Lentinula edodes (L5, L15, and L9)	1474:1524	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	64	theme	djamour	1551:1557	arg1	strain					1531:1536	one strain	1527:1536	one strain of Pleurotus djamour (Pd-UTMR)	1527:1567	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	5	65	theme	Lentinula	1136:1144	arg1	spp					1146:1148	Lentinula spp	1136:1148	Lentinula spp	1136:1148	strains, compared to Lentinula spp.
37256902	1	66	theme	cellulose	692:700	arg1	content					649:655	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	67	theme	ruminal	112:118	arg1	digestibility					129:141	ruminal in vitro digestibility	112:141	ruminal in vitro digestibility	112:141	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	68	theme	fresh	298:302	arg1	mushrooms					304:312	fresh mushrooms	298:312	fresh mushrooms per 100 g of dry substrate	298:339	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	10	69	with	bodies	1834:1839	arg1	Po-IAP					1854:1859	strain Po-IAP	1847:1859	strain Po-IAP as the best yielding	1847:1880	fruiting bodies, with strain Po-IAP as the best yielding.
37256902	1	70	theme	substrate	845:853	arg1	IVDMD					822:826	IVDMD	822:826	IVDMD	822:826	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	70	theme	substrate	845:853	arg1	matter					814:819	dry matter	810:819	dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS),	810:906	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	71	from	effect	73:78	arg1	digestibility					129:141	ruminal in vitro digestibility	112:141	ruminal in vitro digestibility	112:141	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	0	71	from	effect	73:78	arg1	composition					96:106	chemical composition	87:106	chemical composition	87:106	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	72	theme	protein	666:672	arg1	content					649:655	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF)	645:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	73	theme	corn	53:56	arg1	stover					58:63	corn stover	53:63	corn stover	53:63	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	74	theme	dry	327:329	arg1	substrate					331:339	dry substrate	327:339	dry substrate	327:339	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	9	75	theme	corn	1763:1766	arg1	substrate					1789:1797	a suitable substrate	1778:1797	a suitable substrate to produce Pleurotus spp	1778:1822	Our results also showed that corn stover is a suitable substrate to produce Pleurotus spp.
37256902	9	75	theme	corn	1763:1766	arg1	stover					1768:1773	corn stover	1763:1773	corn stover	1763:1773	Our results also showed that corn stover is a suitable substrate to produce Pleurotus spp.
37256902	1	76	theme	in	784:785	arg1	digestibility					793:805	in vitro digestibility	784:805	in vitro digestibility	784:805	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	77	from	LC	569:570	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	8	78	theme	ostreatus	1598:1606	arg1	strain					1578:1583	one strain	1574:1583	one strain of Pleurotus ostreatus (Po-IAP)	1574:1615	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	78	theme	ostreatus	1598:1606	arg1	strains					1480:1486	the 3 strains	1474:1486	the 3 strains of Lentinula edodes (L5, L15, and L9)	1474:1524	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	78	theme	ostreatus	1598:1606	arg1	strain					1531:1536	one strain	1527:1536	one strain of Pleurotus djamour (Pd-UTMR)	1527:1567	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	1	79	theme	biological	265:274	arg1	efficiency					276:285	biological efficiency	265:285	biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO	265:485	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	79	theme	biological	265:274	arg1	Pleurotus					500:508	Pleurotus	500:508	Pleurotus	500:508	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	80	dep	composition	96:106	arg1	the					83:85	the	83:85	the	83:85	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	81	theme	corn	614:617	arg1	stover					619:624	corn stover	614:624	corn stover (CS)	614:629	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	81	theme	corn	614:617	arg1	CS					627:628	CS	627:628	CS	627:628	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	9	82	theme	suitable	1780:1787	arg1	substrate					1789:1797	a suitable substrate	1778:1797	a suitable substrate to produce Pleurotus spp	1778:1822	Our results also showed that corn stover is a suitable substrate to produce Pleurotus spp.
37256902	9	82	theme	suitable	1780:1787	arg1	stover					1768:1773	corn stover	1763:1773	corn stover	1763:1773	Our results also showed that corn stover is a suitable substrate to produce Pleurotus spp.
37256902	1	83	theme	dry	810:812	arg1	IVDMD					822:826	IVDMD	822:826	IVDMD	822:826	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	83	theme	dry	810:812	arg1	matter					814:819	dry matter	810:819	dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS),	810:906	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	7	84	dep	increased	1225:1233	arg1	while					1295:1299	while	1295:1299	while	1295:1299	In comparison to C, the SMS increased the CP content (p < 0.05) from 10.8% (Po-JP) to 70.3% (LC), while NDF decreased (p < 0.05) from 11.5% (Pd-Pro) to 33.5% (L15) and IVDMD increased (p < 0.05) from 16.2% (Heri) to 47.7% (Pd-UTMR).
37256902	8	85	theme	Pleurotus	1541:1549	arg1	Pd-UTMR					1560:1566	Pd-UTMR	1560:1566	Pd-UTMR	1560:1566	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	85	theme	Pleurotus	1541:1549	arg1	djamour					1551:1557	Pleurotus djamour	1541:1557	Pleurotus djamour (Pd-UTMR)	1541:1567	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	1	86	dep	efficiency	276:285	arg1	BE					288:289	BE	288:289	BE	288:289	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	86	dep	efficiency	276:285	arg1	g					293:293	g	293:293	g of fresh mushrooms per 100 g of dry substrate	293:339	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	8	87	used	used	1706:1709	arg2	feed					1714:1717	feed	1714:1717	feed for ruminants	1714:1731	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	8	87	used	used	1706:1709	arg2	SMS					1629:1631	a SMS	1627:1631	a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants	1627:1731	In conclusion, of the 17 strains evaluated, the 3 strains of Lentinula edodes (L5, L15, and L9), one strain of Pleurotus djamour (Pd-UTMR), and one strain of Pleurotus ostreatus (Po-IAP) generated a SMS that, due to its nutritional improvement and increase in IVDMD, could be used as feed for ruminants.
37256902	0	88	theme	strains	17:23	arg1	Production					0:9	Production	0:9	Production of 17 strains of edible mushroom	0:42	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	1	89	theme	detergent	758:766	arg1	fiber					768:772	neutral detergent fiber	750:772	neutral detergent fiber (NDF)	750:778	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	89	theme	detergent	758:766	arg1	NDF					775:777	NDF	775:777	NDF	775:777	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	90	dep	in	784:785	arg1	vitro					787:791	vitro	787:791	vitro	787:791	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	0	91	theme	edible	28:33	arg1	mushroom					35:42	edible mushroom	28:42	edible mushroom	28:42	Production of 17 strains of edible mushroom grown on corn stover and its effect on the chemical composition and ruminal in vitro digestibility of the residual substrate.
37256902	2	92	theme	R	1033:1033	arg1	software					1041:1048	R 4.0.3 software	1033:1048	R 4.0.3 software	1033:1048	The variables were analyzed as a completely randomized block design using R 4.0.3 software.
37256902	1	93	theme	Hericium	577:584	arg1	Heri					596:599	Hericium erinaceus Heri	577:599	Hericium erinaceus Heri	577:599	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	94	theme	Pleurotus	371:379	arg1	Po-IAP					391:396	Pleurotus ostreatus Po-IAP	371:396	Pleurotus ostreatus Po-IAP	371:396	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	94	theme	Pleurotus	371:379	arg1	strains					355:361	17 fungal strains	345:361	17 fungal strains	345:361	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	95	theme	mushrooms	245:253	arg1	/bag					255:258	fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C)	239:956	/bag	255:258	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	96	from	L5	561:562	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	97	from	L9	557:558	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	98	from	L15	552:554	arg1	comparison					911:920	comparison	911:920	comparison to the non-inoculated substrate (C)	911:956	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	99	theme	detergent	723:731	arg1	fiber					733:737	acid detergent fiber	718:737	acid detergent fiber (ADF)	718:743	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	99	theme	detergent	723:731	arg1	ADF					740:742	ADF	740:742	ADF	740:742	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	100	theme	residual	836:843	arg1	substrate					845:853	the residual substrate	832:853	the residual substrate of CS	832:859	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
37256902	1	100	theme	residual	836:843	arg1	substrate					891:899	the so called spent mushroom substrate	862:899	the so called spent mushroom substrate (SMS)	862:905	The objective of this study was to evaluate the production (P) (g of fresh mushrooms /bag) and biological efficiency (BE) (g of fresh mushrooms per 100 g of dry substrate) of 17 fungal strains, namely Pleurotus ostreatus Po-IAP, Po-P38, Po-P14, Po-IE202, Po-Sfco, Po-JP, Po-Psma, and Po-POS, Pleurotus djamour Pd-PRO and Pd-UTMR, Pleurotus eryngii Pe-MB and Pe-PQ, Lentinula edodes L15, L9, L5, and LC, and Hericium erinaceus Heri, produced in corn stover (CS) and to assess the content of crude protein (CP), lignin (L), cellulose, hemicellulose, acid detergent fiber (ADF), and neutral detergent fiber (NDF) and in vitro digestibility of dry matter (IVDMD) of the residual substrate of CS, the so called spent mushroom substrate (SMS), in comparison to the non-inoculated substrate (C).
36748475	3	0	theme	polyphasic	306:315	arg1	study					327:331	a polyphasic taxonomic study	304:331	a polyphasic taxonomic study	304:331	According to the results of a polyphasic taxonomic study, a novel species belonging to the genus Paeniglutamicibacter was described.
36748475	2	1	theme	non-motile	125:134	arg1	bacterium					136:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	3	2	theme	taxonomic	317:325	arg1	study					327:331	a polyphasic taxonomic study	304:331	a polyphasic taxonomic study	304:331	According to the results of a polyphasic taxonomic study, a novel species belonging to the genus Paeniglutamicibacter was described.
36748475	12	3	theme	83.6 	1423:1427	arg1	%					1428:1428	86.6, 74.7 and 83.6 %	1408:1428	%	1428:1428	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	3	theme	83.6 	1423:1427	arg1	values					1273:1278	The average nucleotide identity values	1241:1278	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T	1241:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	4	dep	strains	1318:1324	arg1	SPC26T					1365:1370	SPC26T	1365:1370	SPC26T	1365:1370	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	4	dep	strains	1318:1324	arg1	strains					1318:1324	the type strains	1309:1324	the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T	1309:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	4	dep	strains	1318:1324	arg1	AG31T					1397:1401	AG31T	1397:1401	AG31T	1397:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	4	dep	strains	1318:1324	arg1	DSM20167T					1339:1347	DSM20167T	1339:1347	DSM20167T	1339:1347	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	11	5	theme	%	1238:1238	arg1	content					1219:1225	a DNA G+C content	1209:1225	a DNA G+C content of 66.0 mol%	1209:1238	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	6	6	theme	A4α	600:602	arg1	peptidoglycan					604:616	A4α peptidoglycan	600:616	A4α peptidoglycan (Lys-Glu; A11.54)	600:634	The peptidoglycan structure contained A4α peptidoglycan (Lys-Glu; A11.54).
36748475	7	7	theme	unidentified	738:749	arg1	phospholipids					751:763	unidentified phospholipids	738:763	unidentified phospholipids	738:763	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	2	8	theme	short-rod	111:119	arg1	bacterium					136:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	11	9	theme	66.0 mol	1230:1237	arg1	%					1238:1238	66.0 mol%	1230:1238	66.0 mol%	1230:1238	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	15	10	theme	=TBRC	1658:1662	arg1	ABSL32-1T					1647:1655	ABSL32-1T	1647:1655	ABSL32-1T (=TBRC 14976T=NBRC 115252T)	1647:1683	The type strain is ABSL32-1T (=TBRC 14976T=NBRC 115252T).
36748475	15	10	theme	=TBRC	1658:1662	arg1	115252T					1676:1682	=TBRC 14976T=NBRC 115252T	1658:1682	=TBRC 14976T=NBRC 115252T	1658:1682	The type strain is ABSL32-1T (=TBRC 14976T=NBRC 115252T).
36748475	1	11	theme	midden	59:64	arg1	waste					71:75	midden soil waste	59:75	midden soil waste	59:75	nov., isolated from midden soil waste.
36748475	11	12	theme	strain	1176:1181	arg1	ABSL32-1T					1183:1191	strain ABSL32-1T	1176:1191	strain ABSL32-1T	1176:1191	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	13	13	dep	Paeniglutamicibacter	1584:1603	arg1	quisquiliarum					1605:1617	Paeniglutamicibacter quisquiliarum	1584:1617	Paeniglutamicibacter quisquiliarum	1584:1617	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	9	14	theme	major	842:846	arg1	 0					885:886	 0	885:886	 0	885:886	The major cellular fatty acid was anteiso-C15 : 0 (70.1 %).
36748475	9	14	theme	major	842:846	arg1	acid					863:866	The major cellular fatty acid	838:866	The major cellular fatty acid	838:866	The major cellular fatty acid was anteiso-C15 : 0 (70.1 %).
36748475	10	15	theme	16S	907:909	arg1	analysis					930:937	16S rRNA gene sequence analysis	907:937	16S rRNA gene sequence analysis	907:937	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	1	16	theme	soil	66:69	arg1	waste					71:75	midden soil waste	59:75	midden soil waste	59:75	nov., isolated from midden soil waste.
36748475	2	17	theme	strain	158:163	arg1	ABSL32-1T					165:173	strain ABSL32-1T	158:173	strain ABSL32-1T	158:173	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	9	18	theme	cellular	848:855	arg1	 0					885:886	 0	885:886	 0	885:886	The major cellular fatty acid was anteiso-C15 : 0 (70.1 %).
36748475	9	18	theme	cellular	848:855	arg1	acid					863:866	The major cellular fatty acid	838:866	The major cellular fatty acid	838:866	The major cellular fatty acid was anteiso-C15 : 0 (70.1 %).
36748475	11	19	with	Mbp	1200:1202	arg1	content					1219:1225	a DNA G+C content	1209:1225	a DNA G+C content of 66.0 mol%	1209:1238	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	13	20	theme	novel	1558:1562	arg1	species					1564:1570	a novel species	1556:1570	a novel species to be named Paeniglutamicibacter quisquiliarum sp	1556:1620	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	12	21	theme	nucleotide	1253:1262	arg1	%					1428:1428	86.6, 74.7 and 83.6 %	1408:1428	%	1428:1428	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	21	theme	nucleotide	1253:1262	arg1	values					1273:1278	The average nucleotide identity values	1241:1278	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T	1241:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	3	22	theme	novel	336:340	arg1	species					342:348	a novel species	334:348	a novel species belonging to the genus Paeniglutamicibacter	334:392	According to the results of a polyphasic taxonomic study, a novel species belonging to the genus Paeniglutamicibacter was described.
36748475	12	23	theme	86.6	1408:1411	arg1	%					1428:1428	86.6, 74.7 and 83.6 %	1408:1428	%	1428:1428	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	23	theme	86.6	1408:1411	arg1	values					1273:1278	The average nucleotide identity values	1241:1278	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T	1241:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	9	24	theme	fatty	857:861	arg1	 0					885:886	 0	885:886	 0	885:886	The major cellular fatty acid was anteiso-C15 : 0 (70.1 %).
36748475	9	24	theme	fatty	857:861	arg1	acid					863:866	The major cellular fatty acid	838:866	The major cellular fatty acid	838:866	The major cellular fatty acid was anteiso-C15 : 0 (70.1 %).
36748475	15	25	theme	type	1632:1635	arg1	ABSL32-1T					1647:1655	ABSL32-1T	1647:1655	ABSL32-1T (=TBRC 14976T=NBRC 115252T)	1647:1683	The type strain is ABSL32-1T (=TBRC 14976T=NBRC 115252T).
36748475	15	25	theme	type	1632:1635	arg1	strain					1637:1642	The type strain	1628:1642	The type strain	1628:1642	The type strain is ABSL32-1T (=TBRC 14976T=NBRC 115252T).
36748475	6	26	theme	peptidoglycan	566:578	arg1	structure					580:588	The peptidoglycan structure	562:588	The peptidoglycan structure	562:588	The peptidoglycan structure contained A4α peptidoglycan (Lys-Glu; A11.54).
36748475	4	27	dep	%	492:492	arg1	w/v					495:497	w/v	495:497	w/v	495:497	Strain ABSL32-1T grew optimally at 20-25 °C and at pH 6.0-8.0 in the presence of 1 % (w/v) NaCl.
36748475	0	28	theme	quisquiliarum	21:33	arg1	sp					35:36	Paeniglutamicibacter quisquiliarum sp	0:36	Paeniglutamicibacter quisquiliarum sp.	0:37	Paeniglutamicibacter quisquiliarum sp.
36748475	11	29	theme	ABSL32-1T	1183:1191	arg1	genome					1166:1171	The genome	1162:1171	The genome of strain ABSL32-1T	1162:1191	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	11	29	theme	ABSL32-1T	1183:1191	arg1	Mbp					1200:1202	4.4 Mbp	1196:1202	4.4 Mbp with a DNA G+C content of 66.0 mol%	1196:1238	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	0	30	theme	Paeniglutamicibacter	0:19	arg1	sp					35:36	Paeniglutamicibacter quisquiliarum sp	0:36	Paeniglutamicibacter quisquiliarum sp.	0:37	Paeniglutamicibacter quisquiliarum sp.
36748475	13	31	theme	phenotypic	1461:1470	arg1	properties					1502:1511	phenotypic, chemotaxonomic and genotypic properties	1461:1511	phenotypic, chemotaxonomic and genotypic properties	1461:1511	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	12	32	theme	identity	1264:1271	arg1	%					1428:1428	86.6, 74.7 and 83.6 %	1408:1428	%	1428:1428	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	32	theme	identity	1264:1271	arg1	values					1273:1278	The average nucleotide identity values	1241:1278	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T	1241:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	4	33	theme	1 	490:491	arg1	%					492:492	%	492:492	%	492:492	Strain ABSL32-1T grew optimally at 20-25 °C and at pH 6.0-8.0 in the presence of 1 % (w/v) NaCl.
36748475	13	34	theme	chemotaxonomic	1473:1486	arg1	properties					1502:1511	phenotypic, chemotaxonomic and genotypic properties	1461:1511	phenotypic, chemotaxonomic and genotypic properties	1461:1511	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	13	35	theme	strain	1514:1519	arg1	ABSL32-1T					1521:1529	strain ABSL32-1T	1514:1529	strain ABSL32-1T	1514:1529	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	10	36	theme	sequence	921:928	arg1	analysis					930:937	16S rRNA gene sequence analysis	907:937	16S rRNA gene sequence analysis	907:937	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	12	37	theme	type	1313:1316	arg1	SPC26T					1365:1370	SPC26T	1365:1370	SPC26T	1365:1370	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	37	theme	type	1313:1316	arg1	strains					1318:1324	the type strains	1309:1324	the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T	1309:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	37	theme	type	1313:1316	arg1	AG31T					1397:1401	AG31T	1397:1401	AG31T	1397:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	37	theme	type	1313:1316	arg1	DSM20167T					1339:1347	DSM20167T	1339:1347	DSM20167T	1339:1347	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	2	38	attach	isolated	180:187	arg1	sample					201:206	a soil sample	194:206	a soil sample collected from the Suphan Buri municipal solid waste disposal area	194:273	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	2	38	attach	isolated	180:187	arg2	bacterium					136:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	10	39	theme	gene	916:919	arg1	analysis					930:937	16S rRNA gene sequence analysis	907:937	16S rRNA gene sequence analysis	907:937	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	7	40	contain	contained	654:662	arg2	phosphatidylglycerol					716:735	phosphatidylglycerol	716:735	phosphatidylglycerol	716:735	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	7	40	contain	contained	654:662	arg2	digalactosyldiacylglycerol					664:689	digalactosyldiacylglycerol	664:689	digalactosyldiacylglycerol	664:689	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	7	40	contain	contained	654:662	arg2	diphosphatidylglycerol					692:713	diphosphatidylglycerol	692:713	diphosphatidylglycerol	692:713	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	7	40	contain	contained	654:662	arg2	lipids					786:791	two unidentified lipids	769:791	two unidentified lipids	769:791	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	7	40	contain	contained	654:662	arg1	lipids					647:652	The polar lipids	637:652	The polar lipids	637:652	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	7	40	contain	contained	654:662	arg2	phospholipids					751:763	unidentified phospholipids	738:763	unidentified phospholipids	738:763	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	15	41	theme	14976T=NBRC	1664:1674	arg1	ABSL32-1T					1647:1655	ABSL32-1T	1647:1655	ABSL32-1T (=TBRC 14976T=NBRC 115252T)	1647:1683	The type strain is ABSL32-1T (=TBRC 14976T=NBRC 115252T).
36748475	15	41	theme	14976T=NBRC	1664:1674	arg1	115252T					1676:1682	=TBRC 14976T=NBRC 115252T	1658:1682	=TBRC 14976T=NBRC 115252T	1658:1682	The type strain is ABSL32-1T (=TBRC 14976T=NBRC 115252T).
36748475	2	42	theme	Buri	234:237	arg1	area					270:273	the Suphan Buri municipal solid waste disposal area	223:273	the Suphan Buri municipal solid waste disposal area	223:273	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	13	43	dep	properties	1502:1511	arg1	the					1448:1450	the	1448:1450	the	1448:1450	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	13	43	dep	properties	1502:1511	arg1	basis					1452:1456	basis	1452:1456	basis	1452:1456	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	13	44	theme	genotypic	1492:1500	arg1	properties					1502:1511	phenotypic, chemotaxonomic and genotypic properties	1461:1511	phenotypic, chemotaxonomic and genotypic properties	1461:1511	On the basis of phenotypic, chemotaxonomic and genotypic properties, strain ABSL32-1T is proposed to represent a novel species to be named Paeniglutamicibacter quisquiliarum sp.
36748475	4	45	theme	NaCl	500:503	arg1	presence					478:485	the presence	474:485	the presence of 1 % (w/v) NaCl	474:503	Strain ABSL32-1T grew optimally at 20-25 °C and at pH 6.0-8.0 in the presence of 1 % (w/v) NaCl.
36748475	5	46	theme	whole-cell	510:519	arg1	sugars					521:526	The whole-cell sugars	506:526	The whole-cell sugars	506:526	The whole-cell sugars were ribose, mannose and glucose.
36748475	5	46	theme	whole-cell	510:519	arg1	ribose					533:538	ribose	533:538	ribose	533:538	The whole-cell sugars were ribose, mannose and glucose.
36748475	6	47	contain	contained	590:598	arg2	peptidoglycan					604:616	A4α peptidoglycan	600:616	A4α peptidoglycan (Lys-Glu; A11.54)	600:634	The peptidoglycan structure contained A4α peptidoglycan (Lys-Glu; A11.54).
36748475	6	47	contain	contained	590:598	arg1	structure					580:588	The peptidoglycan structure	562:588	The peptidoglycan structure	562:588	The peptidoglycan structure contained A4α peptidoglycan (Lys-Glu; A11.54).
36748475	10	48	theme	sulfureus	1011:1019	arg1	%					1038:1038	99.5 %	1033:1038	99.5 %	1033:1038	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	10	48	theme	sulfureus	1011:1019	arg1	DSM					1021:1023	Paeniglutamicibacter sulfureus DSM 20167T	990:1030	Paeniglutamicibacter sulfureus DSM 20167T (99.5 %)	990:1039	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	2	49	theme	Gram-stain-positive	80:98	arg1	bacterium					136:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium	78:144	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	4	50	theme	%	492:492	arg1	NaCl					500:503	1 % (w/v) NaCl	490:503	1 % (w/v) NaCl	490:503	Strain ABSL32-1T grew optimally at 20-25 °C and at pH 6.0-8.0 in the presence of 1 % (w/v) NaCl.
36748475	7	51	theme	polar	641:645	arg1	lipids					647:652	The polar lipids	637:652	The polar lipids	637:652	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	10	52	theme	strain	940:945	arg1	ABSL32-1T					947:955	strain ABSL32-1T	940:955	strain ABSL32-1T	940:955	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	10	53	theme	highest	968:974	arg1	similarity					976:985	the highest similarity	964:985	the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %)	964:1039	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	11	54	theme	DNA	1211:1213	arg1	content					1219:1225	a DNA G+C content	1209:1225	a DNA G+C content of 66.0 mol%	1209:1238	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	10	55	theme	Paeniglutamicibacter	990:1009	arg1	%					1038:1038	99.5 %	1033:1038	99.5 %	1033:1038	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	10	55	theme	Paeniglutamicibacter	990:1009	arg1	DSM					1021:1023	Paeniglutamicibacter sulfureus DSM 20167T	990:1030	Paeniglutamicibacter sulfureus DSM 20167T (99.5 %)	990:1039	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36748475	2	56	theme	disposal	261:268	arg1	area					270:273	the Suphan Buri municipal solid waste disposal area	223:273	the Suphan Buri municipal solid waste disposal area	223:273	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	6	57	dep	peptidoglycan	604:616	arg1	Lys-Glu					619:625	Lys-Glu	619:625	Lys-Glu; A11.54	619:633	The peptidoglycan structure contained A4α peptidoglycan (Lys-Glu; A11.54).
36748475	11	58	theme	G+C	1215:1217	arg1	content					1219:1225	a DNA G+C content	1209:1225	a DNA G+C content of 66.0 mol%	1209:1238	The genome of strain ABSL32-1T is 4.4 Mbp with a DNA G+C content of 66.0 mol%.
36748475	2	59	theme	soil	196:199	arg1	sample					201:206	a soil sample	194:206	a soil sample collected from the Suphan Buri municipal solid waste disposal area	194:273	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	2	60	theme	waste	255:259	arg1	area					270:273	the Suphan Buri municipal solid waste disposal area	223:273	the Suphan Buri municipal solid waste disposal area	223:273	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	7	61	theme	unidentified	773:784	arg1	lipids					786:791	two unidentified lipids	769:791	two unidentified lipids	769:791	The polar lipids contained digalactosyldiacylglycerol, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and two unidentified lipids.
36748475	3	62	theme	study	327:331	arg1	results					293:299	the results	289:299	the results of a polyphasic taxonomic study	289:331	According to the results of a polyphasic taxonomic study, a novel species belonging to the genus Paeniglutamicibacter was described.
36748475	2	63	theme	solid	249:253	arg1	area					270:273	the Suphan Buri municipal solid waste disposal area	223:273	the Suphan Buri municipal solid waste disposal area	223:273	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	6	64	dep	Lys-Glu	619:625	arg1	A11.54					628:633	A11.54	628:633	Lys-Glu; A11.54	619:633	The peptidoglycan structure contained A4α peptidoglycan (Lys-Glu; A11.54).
36748475	12	65	theme	average	1245:1251	arg1	%					1428:1428	86.6, 74.7 and 83.6 %	1408:1428	%	1428:1428	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	12	65	theme	average	1245:1251	arg1	values					1273:1278	The average nucleotide identity values	1241:1278	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T	1241:1401	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	8	66	theme	major	798:802	arg1	MK-9					822:825	MK-9	822:825	MK-9	822:825	The major menaquinones were MK-9 and MK-10.
36748475	8	66	theme	major	798:802	arg1	menaquinones					804:815	The major menaquinones	794:815	The major menaquinones	794:815	The major menaquinones were MK-9 and MK-10.
36748475	2	67	dep	Gram-stain-positive	80:98	arg1	irregular					101:109	irregular	101:109	irregular	101:109	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	2	68	theme	municipal	239:247	arg1	area					270:273	the Suphan Buri municipal solid waste disposal area	223:273	the Suphan Buri municipal solid waste disposal area	223:273	A Gram-stain-positive, irregular short-rod and non-motile bacterium, designated strain ABSL32-1T, was isolated from a soil sample collected from the Suphan Buri municipal solid waste disposal area.
36748475	12	69	theme	strain	1288:1293	arg1	ABSL32-1T					1295:1303	strain ABSL32-1T	1288:1303	strain ABSL32-1T	1288:1303	The average nucleotide identity values between strain ABSL32-1T and the type strains P. sulfureus DSM20167T, P. antarcticus SPC26T and P. psychrophenolicus AG31T were 86.6, 74.7 and 83.6 %, respectively.
36748475	3	70	theme	genus	367:371	arg1	Paeniglutamicibacter					373:392	the genus Paeniglutamicibacter	363:392	the genus Paeniglutamicibacter	363:392	According to the results of a polyphasic taxonomic study, a novel species belonging to the genus Paeniglutamicibacter was described.
36748475	10	71	theme	rRNA	911:914	arg1	analysis					930:937	16S rRNA gene sequence analysis	907:937	16S rRNA gene sequence analysis	907:937	Based on 16S rRNA gene sequence analysis, strain ABSL32-1T showed the highest similarity to Paeniglutamicibacter sulfureus DSM 20167T (99.5 %), followed by Paeniglutamicibacter antarcticus SPC26T (99.0 %) and Paeniglutamicibacter psychrophenolicus AG31T (98.8 %).
36293517	9	0	theme	high	1325:1328	arg1	connectivity					1330:1341	high connectivity	1325:1341	high connectivity	1325:1341	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	7	1	theme	selection	1152:1160	arg1	GLASSO					1172:1177	GLASSO	1172:1177	GLASSO	1172:1177	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	7	1	theme	selection	1152:1160	arg1	operator					1162:1169	selection operator	1152:1169	selection operator (GLASSO)	1152:1178	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	9	2	theme	predicted	1358:1366	arg1	functions					1368:1376	predicted functions	1358:1376	predicted functions associated with ABA response in grain	1358:1414	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	6	3	theme	RNAseq	926:931	arg1	datasets					933:940	publicly available RNAseq datasets	907:940	publicly available RNAseq datasets	907:940	To explore the regulatory variation, weighted correlation gene network was constructed from publicly available RNAseq datasets to identify the modules differentially regulated in TAM 111 and TAM 112.
36293517	0	4	from	Analysis	14:21	arg1	TAM					65:67	TAM	65:67	TAM	65:67	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	4	from	Analysis	14:21	arg1	TAM					77:79	TAM	77:79	TAM	77:79	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	4	from	Analysis	14:21	arg1	Cultivars					55:63	Wheat Cultivars	49:63	Wheat Cultivars TAM 111 and TAM 112	49:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	6	5	theme	regulatory	830:839	arg1	variation					841:849	the regulatory variation	826:849	the regulatory variation	826:849	To explore the regulatory variation, weighted correlation gene network was constructed from publicly available RNAseq datasets to identify the modules differentially regulated in TAM 111 and TAM 112.
36293517	2	6	theme	Great	320:324	arg1	Plains					326:331	the Southern US Great Plains	304:331	the Southern US Great Plains with significantly different protein content	304:376	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	4	7	theme	metabolism-related	576:593	arg1	pathways					595:602	starch metabolism-related pathways	569:602	starch metabolism-related pathways	569:602	At the mid-filling stage, TAM 111 preferentially upregulated starch metabolism-related pathways compared to TAM 112, whereas amino acid metabolism and transporter-related pathways were over-represented in TAM 112.
36293517	7	8	dep	graphical	1113:1121	arg1	absolute					1129:1136	absolute	1129:1136	absolute	1129:1136	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	9	9	from	response	1398:1405	arg1	grain					1410:1414	grain	1410:1414	grain	1410:1414	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	5	10	theme	higher	758:763	arg1	percentage					767:776	a higher N percentage	756:776	a higher N percentage in TAM 112	756:787	Elemental analyses also indicated a higher N percentage in TAM 112 at the mid-filling stage.
36293517	0	11	from	Cultivars	55:63	arg1	Analysis					14:21	Transcriptome Analysis	0:21	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112	0:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	11	from	Cultivars	55:63	arg1	Grains					37:42	Developing Grains	26:42	Developing Grains from Wheat Cultivars TAM 111 and TAM 112	26:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	12	theme	Regulatory	110:119	arg1	Networks					121:128	Cultivar-Specific Regulatory Networks	92:128	Cultivar-Specific Regulatory Networks	92:128	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	6	13	theme	available	916:924	arg1	datasets					933:940	publicly available RNAseq datasets	907:940	publicly available RNAseq datasets	907:940	To explore the regulatory variation, weighted correlation gene network was constructed from publicly available RNAseq datasets to identify the modules differentially regulated in TAM 111 and TAM 112.
36293517	8	14	theme	OsNF-Y	1197:1202	arg1	members					1211:1217	the OsNF-Y family members	1193:1217	the OsNF-Y family members	1193:1217	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	9	15	with	TFs	1316:1318	arg1	connectivity					1330:1341	high connectivity	1325:1341	high connectivity	1325:1341	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	0	16	theme	Cultivar-Specific	92:108	arg1	Networks					121:128	Cultivar-Specific Regulatory Networks	92:128	Cultivar-Specific Regulatory Networks	92:128	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	8	17	theme	metabolism-related	1237:1254	arg1	functions					1256:1264	known starch metabolism-related functions	1224:1264	known starch metabolism-related functions	1224:1264	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	8	18	theme	family	1204:1209	arg1	members					1211:1217	the OsNF-Y family members	1193:1217	the OsNF-Y family members	1193:1217	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	4	19	theme	transporter-related	659:677	arg1	pathways					679:686	transporter-related pathways	659:686	transporter-related pathways	659:686	At the mid-filling stage, TAM 111 preferentially upregulated starch metabolism-related pathways compared to TAM 112, whereas amino acid metabolism and transporter-related pathways were over-represented in TAM 112.
36293517	8	20	theme	members	1211:1217	arg1	Homologs					1181:1188	Homologs	1181:1188	Homologs of the OsNF-Y family members with known starch metabolism-related functions	1181:1264	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	9	21	theme	ABA	1394:1396	arg1	response					1398:1405	ABA response	1394:1405	ABA response in grain	1394:1414	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	2	22	theme	protein	362:368	arg1	content					370:376	significantly different protein content	338:376	significantly different protein content	338:376	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	0	23	theme	Transcriptome	0:12	arg1	Analysis					14:21	Transcriptome Analysis	0:21	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112	0:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	7	24	theme	graphical	1113:1121	arg1	shrinkage					1138:1146	shrinkage	1138:1146	shrinkage	1138:1146	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	6	25	theme	correlation	861:871	arg1	network					878:884	weighted correlation gene network	852:884	weighted correlation gene network	852:884	To explore the regulatory variation, weighted correlation gene network was constructed from publicly available RNAseq datasets to identify the modules differentially regulated in TAM 111 and TAM 112.
36293517	2	26	theme	different	352:360	arg1	content					370:376	significantly different protein content	338:376	significantly different protein content	338:376	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	0	27	theme	Developing	26:35	arg1	Grains					37:42	Developing Grains	26:42	Developing Grains from Wheat Cultivars TAM 111 and TAM 112	26:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	5	28	theme	Elemental	722:730	arg1	analyses					732:739	Elemental analyses	722:739	Elemental analyses	722:739	Elemental analyses also indicated a higher N percentage in TAM 112 at the mid-filling stage.
36293517	8	29	with	Homologs	1181:1188	arg1	functions					1256:1264	known starch metabolism-related functions	1224:1264	known starch metabolism-related functions	1224:1264	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	2	30	theme	popular	277:283	arg1	TAM					249:251	TAM	249:251	TAM	249:251	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	2	30	theme	popular	277:283	arg1	cultivars					285:293	two popular cultivars	273:293	two popular cultivars grown in the Southern US Great Plains with significantly different protein content	273:376	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	2	30	theme	popular	277:283	arg1	TAM					261:263	TAM	261:263	TAM	261:263	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	9	31	contain	had	1354:1356	arg2	functions					1368:1376	predicted functions	1358:1376	predicted functions associated with ABA response in grain	1358:1414	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	9	31	contain	had	1354:1356	arg1	TFs					1316:1318	Multiple TFs	1307:1318	Multiple TFs with high connectivity in TAM 112	1307:1352	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	3	32	theme	developing	456:465	arg1	grains					467:472	developing grains	456:472	developing grains	456:472	To investigate regulatory differences, transcriptome data were analyzed from developing grains at early- and mid-filling stages.
36293517	4	33	theme	starch	569:574	arg1	pathways					595:602	starch metabolism-related pathways	569:602	starch metabolism-related pathways	569:602	At the mid-filling stage, TAM 111 preferentially upregulated starch metabolism-related pathways compared to TAM 112, whereas amino acid metabolism and transporter-related pathways were over-represented in TAM 112.
36293517	8	34	from	connectivities	1280:1293	arg1	TAM					1298:1300	TAM 111	1298:1304	TAM 111	1298:1304	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	1	35	attach	linked	172:177	arg2	quality					153:159	Wheat flour's end-use quality	131:159	Wheat flour's end-use quality	131:159	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	1	35	attach	linked	172:177	arg1	composition					199:209	composition	199:209	composition	199:209	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	1	35	attach	linked	172:177	arg1	quantity					186:193	quantity	186:193	quantity	186:193	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	2	36	dep	Southern	308:315	arg1	US					317:318	US	317:318	US	317:318	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	4	37	theme	amino	633:637	arg1	metabolism					644:653	amino acid metabolism	633:653	amino acid metabolism	633:653	At the mid-filling stage, TAM 111 preferentially upregulated starch metabolism-related pathways compared to TAM 112, whereas amino acid metabolism and transporter-related pathways were over-represented in TAM 112.
36293517	3	38	theme	regulatory	394:403	arg1	differences					405:415	regulatory differences	394:415	regulatory differences	394:415	To investigate regulatory differences, transcriptome data were analyzed from developing grains at early- and mid-filling stages.
36293517	3	39	theme	mid-filling	488:498	arg1	stages					500:505	early- and mid-filling stages	477:505	early- and mid-filling stages	477:505	To investigate regulatory differences, transcriptome data were analyzed from developing grains at early- and mid-filling stages.
36293517	10	40	theme	grain	1537:1541	arg1	development					1543:1553	grain development	1537:1553	grain development	1537:1553	These results will provide novel targets for breeders to explore and further our understanding in mechanisms regulating grain development.
36293517	5	41	from	percentage	767:776	arg1	TAM					781:783	TAM 112	781:787	TAM 112	781:787	Elemental analyses also indicated a higher N percentage in TAM 112 at the mid-filling stage.
36293517	0	42	dep	Cultivars	55:63	arg1	TAM					65:67	TAM	65:67	TAM	65:67	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	42	dep	Cultivars	55:63	arg1	TAM					77:79	TAM	77:79	TAM	77:79	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	42	dep	Cultivars	55:63	arg1	Cultivars					55:63	Wheat Cultivars	49:63	Wheat Cultivars TAM 111 and TAM 112	49:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	3	43	theme	transcriptome	418:430	arg1	data					432:435	transcriptome data	418:435	transcriptome data	418:435	To investigate regulatory differences, transcriptome data were analyzed from developing grains at early- and mid-filling stages.
36293517	0	44	theme	Grains	37:42	arg1	Analysis					14:21	Transcriptome Analysis	0:21	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112	0:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	10	45	from	understanding	1498:1510	arg1	mechanisms					1515:1524	mechanisms	1515:1524	mechanisms regulating grain development	1515:1553	These results will provide novel targets for breeders to explore and further our understanding in mechanisms regulating grain development.
36293517	5	46	theme	mid-filling	796:806	arg1	stage					808:812	the mid-filling stage	792:812	the mid-filling stage	792:812	Elemental analyses also indicated a higher N percentage in TAM 112 at the mid-filling stage.
36293517	8	47	theme	known	1224:1228	arg1	functions					1256:1264	known starch metabolism-related functions	1224:1264	known starch metabolism-related functions	1224:1264	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	1	48	from	composition	199:209	arg1	endosperm					238:246	the endosperm	234:246	the endosperm	234:246	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	4	49	theme	mid-filling	515:525	arg1	stage					527:531	the mid-filling stage	511:531	the mid-filling stage	511:531	At the mid-filling stage, TAM 111 preferentially upregulated starch metabolism-related pathways compared to TAM 112, whereas amino acid metabolism and transporter-related pathways were over-represented in TAM 112.
36293517	8	50	theme	starch	1230:1235	arg1	functions					1256:1264	known starch metabolism-related functions	1224:1264	known starch metabolism-related functions	1224:1264	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	6	51	theme	gene	873:876	arg1	network					878:884	weighted correlation gene network	852:884	weighted correlation gene network	852:884	To explore the regulatory variation, weighted correlation gene network was constructed from publicly available RNAseq datasets to identify the modules differentially regulated in TAM 111 and TAM 112.
36293517	0	52	theme	Wheat	49:53	arg1	TAM					65:67	TAM	65:67	TAM	65:67	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	52	theme	Wheat	49:53	arg1	TAM					77:79	TAM	77:79	TAM	77:79	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	0	52	theme	Wheat	49:53	arg1	Cultivars					55:63	Wheat Cultivars	49:63	Wheat Cultivars TAM 111 and TAM 112	49:83	Transcriptome Analysis of Developing Grains from Wheat Cultivars TAM 111 and TAM 112 Reveal Cultivar-Specific Regulatory Networks.
36293517	2	53	with	Plains	326:331	arg1	content					370:376	significantly different protein content	338:376	significantly different protein content	338:376	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	1	54	from	quantity	186:193	arg1	endosperm					238:246	the endosperm	234:246	the endosperm	234:246	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	2	55	theme	Southern	308:315	arg1	Plains					326:331	the Southern US Great Plains	304:331	the Southern US Great Plains with significantly different protein content	304:376	TAM 111 and TAM 112 are two popular cultivars grown in the Southern US Great Plains with significantly different protein content.
36293517	10	56	theme	novel	1444:1448	arg1	targets					1450:1456	novel targets	1444:1456	novel targets for breeders to explore and further our understanding in mechanisms regulating grain development	1444:1553	These results will provide novel targets for breeders to explore and further our understanding in mechanisms regulating grain development.
36293517	6	57	theme	weighted	852:859	arg1	network					878:884	weighted correlation gene network	852:884	weighted correlation gene network	852:884	To explore the regulatory variation, weighted correlation gene network was constructed from publicly available RNAseq datasets to identify the modules differentially regulated in TAM 111 and TAM 112.
36293517	7	58	theme	potential	1028:1036	arg1	TFs					1061:1063	TFs	1061:1063	TFs	1061:1063	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	7	58	theme	potential	1028:1036	arg1	factors					1052:1058	the potential transcription factors	1024:1058	the potential transcription factors (TFs) regulating those modules	1024:1089	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	1	59	theme	Wheat	131:135	arg1	flour					137:141	Wheat flour's	131:143	Wheat flour's end-use quality	131:159	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	1	60	theme	storage	214:220	arg1	proteins					222:229	storage proteins	214:229	storage proteins	214:229	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	9	61	theme	Multiple	1307:1314	arg1	TFs					1316:1318	Multiple TFs	1307:1318	Multiple TFs with high connectivity in TAM 112	1307:1352	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	7	62	theme	transcription	1038:1050	arg1	TFs					1061:1063	TFs	1061:1063	TFs	1061:1063	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	7	62	theme	transcription	1038:1050	arg1	factors					1052:1058	the potential transcription factors	1024:1058	the potential transcription factors (TFs) regulating those modules	1024:1089	Further, the potential transcription factors (TFs) regulating those modules were identified using graphical least absolute shrinkage and selection operator (GLASSO).
36293517	3	63	theme	early-	477:482	arg1	stages					500:505	early- and mid-filling stages	477:505	early- and mid-filling stages	477:505	To investigate regulatory differences, transcriptome data were analyzed from developing grains at early- and mid-filling stages.
36293517	1	64	theme	proteins	222:229	arg1	composition					199:209	composition	199:209	composition	199:209	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	1	64	theme	proteins	222:229	arg1	quantity					186:193	quantity	186:193	quantity	186:193	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	1	65	dep	quantity	186:193	arg1	the					182:184	the	182:184	the	182:184	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	4	66	theme	acid	639:642	arg1	metabolism					644:653	amino acid metabolism	633:653	amino acid metabolism	633:653	At the mid-filling stage, TAM 111 preferentially upregulated starch metabolism-related pathways compared to TAM 112, whereas amino acid metabolism and transporter-related pathways were over-represented in TAM 112.
36293517	8	67	theme	higher	1273:1278	arg1	connectivities					1280:1293	higher connectivities	1273:1293	higher connectivities in TAM 111	1273:1304	Homologs of the OsNF-Y family members with known starch metabolism-related functions showed higher connectivities in TAM 111.
36293517	9	68	from	TFs	1316:1318	arg1	TAM					1346:1348	TAM 112	1346:1352	TAM 112	1346:1352	Multiple TFs with high connectivity in TAM 112 had predicted functions associated with ABA response in grain.
36293517	1	69	theme	end-use	145:151	arg1	quality					153:159	Wheat flour's end-use quality	131:159	Wheat flour's end-use quality	131:159	Wheat flour's end-use quality is tightly linked to the quantity and composition of storage proteins in the endosperm.
36293517	5	70	theme	N	765:765	arg1	percentage					767:776	a higher N percentage	756:776	a higher N percentage in TAM 112	756:787	Elemental analyses also indicated a higher N percentage in TAM 112 at the mid-filling stage.
36282549	2	0	theme	salt-tolerant	113:125	arg1	bacterium					127:135	A salt-tolerant bacterium	111:135	A salt-tolerant bacterium	111:135	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	5	1	theme	phenotypic	669:678	arg1	characteristics					709:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	The novel isolate could be distinguished from species of the genus Pseudalkalibacillus by its distinct phenotypic, physiological and genotypic characteristics.
36282549	13	2	theme	type	1487:1490	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	2	theme	type	1487:1490	arg1	strain					1492:1497	The type strain	1483:1497	The type strain of the proposed novel isolate	1483:1527	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	5	3	theme	genotypic	699:707	arg1	characteristics					709:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	The novel isolate could be distinguished from species of the genus Pseudalkalibacillus by its distinct phenotypic, physiological and genotypic characteristics.
36282549	10	4	theme	data	1196:1199	arg1	%					1247:1247	41.6 %	1242:1247	41.6 %	1242:1247	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	4	theme	data	1196:1199	arg1	content					1214:1220	the DNA G+C content	1202:1220	the DNA G+C content of EGI L200015T	1202:1236	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	4	theme	data	1196:1199	arg1	data					1196:1199	the genome sequence data	1176:1199	the genome sequence data	1176:1199	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	4	5	theme	sequence	538:545	arg1	identity					547:554	sequence identity	538:554	sequence identity of 98.3%	538:563	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	11	6	theme	name	1425:1428	arg1	sp					1461:1462	the name Pseudalkalibacillus salsuginis sp	1421:1462	the name Pseudalkalibacillus salsuginis sp	1421:1462	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	4	7	with	clade	480:484	arg1	identity					547:554	sequence identity	538:554	sequence identity of 98.3%	538:563	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	4	7	with	clade	480:484	arg1	KCTC					521:524	Pseudalkalibacillus berkeleyi KCTC 12718T	491:531	Pseudalkalibacillus berkeleyi KCTC 12718T	491:531	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	10	8	theme	sequence	1187:1194	arg1	data					1196:1199	the genome sequence data	1176:1199	the genome sequence data	1176:1199	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	3	9	theme	phylogenomic	349:360	arg1	analysis					362:369	phylogenomic analysis	349:369	phylogenomic analysis	349:369	The taxonomic position of the isolate was determined using polyphasic taxonomic analysis and phylogenomic analysis.
36282549	5	10	theme	physiological	681:693	arg1	characteristics					709:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	The novel isolate could be distinguished from species of the genus Pseudalkalibacillus by its distinct phenotypic, physiological and genotypic characteristics.
36282549	4	11	theme	16S	398:400	arg1	rRNA					402:405	16S rRNA	398:405	16S rRNA	398:405	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	1	12	theme	salt-tolerant	49:61	arg1	bacterium					63:71	a novel salt-tolerant bacterium	41:71	a novel salt-tolerant bacterium	41:71	nov., a novel salt-tolerant bacterium isolated from a saline lake sediment.
36282549	1	12	theme	salt-tolerant	49:61	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel salt-tolerant bacterium isolated from a saline lake sediment.
36282549	10	13	theme	genome	1180:1185	arg1	data					1196:1199	the genome sequence data	1176:1199	the genome sequence data	1176:1199	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	11	14	theme	novel	1363:1367	arg1	species					1369:1375	a novel species	1361:1375	a novel species	1361:1375	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	2	15	theme	EGI	156:158	arg1	L200015T					160:167	strain EGI L200015T	149:167	strain EGI L200015T	149:167	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	4	16	theme	sequence	412:419	arg1	similarities					421:432	gene sequence similarities	407:432	gene sequence similarities	407:432	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	9	17	theme	polar	1060:1064	arg1	lipids					1066:1071	The detected polar lipids	1047:1071	The detected polar lipids of	1047:1074	The detected polar lipids of included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
36282549	2	18	from	sediment	200:207	arg1	China					249:253	China	249:253	China	249:253	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	2	19	theme	strain	149:154	arg1	L200015T					160:167	strain EGI L200015T	149:167	strain EGI L200015T	149:167	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	5	20	theme	Pseudalkalibacillus	633:651	arg1	species					612:618	species	612:618	species of the genus Pseudalkalibacillus	612:651	The novel isolate could be distinguished from species of the genus Pseudalkalibacillus by its distinct phenotypic, physiological and genotypic characteristics.
36282549	4	21	theme	gene	407:410	arg1	similarities					421:432	gene sequence similarities	407:432	gene sequence similarities	407:432	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	13	22	theme	=	1546:1546	arg1	1.19260T					1568:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	22	theme	=	1546:1546	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	6	23	theme	L200015T	739:746	arg1	Cells					726:730	Cells	726:730	Cells of EGI L200015T	726:746	Cells of EGI L200015T were aerobic, Gram-stain-positive, non-motile and rod-shaped.
36282549	4	24	theme	berkeleyi	511:519	arg1	KCTC					521:524	Pseudalkalibacillus berkeleyi KCTC 12718T	491:531	Pseudalkalibacillus berkeleyi KCTC 12718T	491:531	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	4	25	theme	Phylogenetic	372:383	arg1	analysis					385:392	Phylogenetic analysis	372:392	Phylogenetic analysis	372:392	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	11	26	theme	physiological	1282:1294	arg1	data					1324:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	8	27	theme	major	906:910	arg1	quinone					924:930	The major respiratory quinone	902:930	The major respiratory quinone	902:930	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	27	theme	major	906:910	arg1	MK-7					936:939	MK-7	936:939	MK-7	936:939	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	7	28	theme	Optimal	810:816	arg1	conditions					825:834	Optimal growth conditions	810:834	Optimal growth conditions for EGI L200015T	810:851	Optimal growth conditions for EGI L200015T occurred on marine agar 2216 at pH 8.0 at 30 °C.
36282549	0	29	theme	salsuginis	20:29	arg1	sp					31:32	Pseudalkalibacillus salsuginis sp	0:32	Pseudalkalibacillus salsuginis sp.	0:33	Pseudalkalibacillus salsuginis sp.
36282549	4	30	theme	Pseudalkalibacillus	491:509	arg1	KCTC					521:524	Pseudalkalibacillus berkeleyi KCTC 12718T	491:531	Pseudalkalibacillus berkeleyi KCTC 12718T	491:531	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	11	31	theme	Pseudalkalibacillus	1390:1408	arg1	species					1369:1375	a novel species	1361:1375	a novel species	1361:1375	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	11	32	theme	strain	1330:1335	arg1	L200015T					1341:1348	strain EGI L200015T	1330:1348	strain EGI L200015T	1330:1348	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	13	33	theme	novel	1515:1519	arg1	isolate					1521:1527	the proposed novel isolate	1502:1527	the proposed novel isolate	1502:1527	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	8	34	dep	anteiso-C15	984:994	arg1	0					1044:1044	0	1044:1044	0	1044:1044	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	34	dep	anteiso-C15	984:994	arg1	0					1024:1024	0	1024:1024	0	1024:1024	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	34	dep	anteiso-C15	984:994	arg1	anteiso-C17					1030:1040	anteiso-C17	1030:1040	anteiso-C17	1030:1040	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	34	dep	anteiso-C15	984:994	arg1	0					998:998	0	998:998	0	998:998	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	34	dep	anteiso-C15	984:994	arg1	iso-C16					1014:1020	iso-C16	1014:1020	iso-C16	1014:1020	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	0	35	theme	Pseudalkalibacillus	0:18	arg1	sp					31:32	Pseudalkalibacillus salsuginis sp	0:32	Pseudalkalibacillus salsuginis sp.	0:33	Pseudalkalibacillus salsuginis sp.
36282549	5	36	theme	distinct	660:667	arg1	characteristics					709:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	its distinct phenotypic, physiological and genotypic characteristics	656:723	The novel isolate could be distinguished from species of the genus Pseudalkalibacillus by its distinct phenotypic, physiological and genotypic characteristics.
36282549	1	37	theme	saline	89:94	arg1	sediment					101:108	a saline lake sediment	87:108	a saline lake sediment	87:108	nov., a novel salt-tolerant bacterium isolated from a saline lake sediment.
36282549	3	38	theme	taxonomic	260:268	arg1	position					270:277	The taxonomic position	256:277	The taxonomic position of the isolate	256:292	The taxonomic position of the isolate was determined using polyphasic taxonomic analysis and phylogenomic analysis.
36282549	5	39	theme	genus	627:631	arg1	Pseudalkalibacillus					633:651	the genus Pseudalkalibacillus	623:651	the genus Pseudalkalibacillus	623:651	The novel isolate could be distinguished from species of the genus Pseudalkalibacillus by its distinct phenotypic, physiological and genotypic characteristics.
36282549	1	40	theme	lake	96:99	arg1	sediment					101:108	a saline lake sediment	87:108	a saline lake sediment	87:108	nov., a novel salt-tolerant bacterium isolated from a saline lake sediment.
36282549	8	41	theme	respiratory	912:922	arg1	quinone					924:930	The major respiratory quinone	902:930	The major respiratory quinone	902:930	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	41	theme	respiratory	912:922	arg1	MK-7					936:939	MK-7	936:939	MK-7	936:939	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	11	42	theme	phenotypic	1270:1279	arg1	data					1324:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	11	43	theme	salsuginis	1450:1459	arg1	sp					1461:1462	the name Pseudalkalibacillus salsuginis sp	1421:1462	the name Pseudalkalibacillus salsuginis sp	1421:1462	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	8	44	theme	major	952:956	arg1	acids					964:968	the major fatty acids	948:968	the major fatty acids (> 10 %)	948:977	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	44	theme	major	952:956	arg1	%					976:976	> 10 %	971:976	> 10 %	971:976	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	44	theme	major	952:956	arg1	anteiso-C15					984:994	anteiso-C15	984:994	anteiso-C15	984:994	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	13	45	theme	EGI	1532:1534	arg1	1.19260T					1568:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	45	theme	EGI	1532:1534	arg1	strain					1492:1497	The type strain	1483:1497	The type strain of the proposed novel isolate	1483:1527	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	45	theme	EGI	1532:1534	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	7	46	theme	marine	865:870	arg1	2216					877:880	marine agar 2216	865:880	marine agar 2216	865:880	Optimal growth conditions for EGI L200015T occurred on marine agar 2216 at pH 8.0 at 30 °C.
36282549	2	47	attach	isolated	174:181	arg2	bacterium					127:135	A salt-tolerant bacterium	111:135	A salt-tolerant bacterium	111:135	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	2	47	attach	isolated	174:181	arg1	sediment					200:207	saline lake sediment	188:207	saline lake sediment in Xinjiang Uygur Autonomous Region, PR China	188:253	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	11	48	theme	genus	1384:1388	arg1	Pseudalkalibacillus					1390:1408	the genus Pseudalkalibacillus	1380:1408	the genus Pseudalkalibacillus	1380:1408	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	2	49	theme	Autonomous	227:236	arg1	Region					238:243	Autonomous Region	227:243	Xinjiang Uygur Autonomous Region	212:243	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	7	50	theme	agar	872:875	arg1	2216					877:880	marine agar 2216	865:880	marine agar 2216	865:880	Optimal growth conditions for EGI L200015T occurred on marine agar 2216 at pH 8.0 at 30 °C.
36282549	11	51	theme	Pseudalkalibacillus	1430:1448	arg1	sp					1461:1462	the name Pseudalkalibacillus salsuginis sp	1421:1462	the name Pseudalkalibacillus salsuginis sp	1421:1462	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	11	52	theme	phylogenetic	1311:1322	arg1	data					1324:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	4	53	theme	distinct	471:478	arg1	clade					480:484	a distinct clade	469:484	a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%	469:563	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	13	54	theme	43363T	1553:1558	arg1	1.19260T					1568:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	54	theme	43363T	1553:1558	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	2	55	dep	Uygur	221:225	arg1	Region					238:243	Autonomous Region	227:243	Xinjiang Uygur Autonomous Region	212:243	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	3	56	theme	isolate	286:292	arg1	position					270:277	The taxonomic position	256:277	The taxonomic position of the isolate	256:292	The taxonomic position of the isolate was determined using polyphasic taxonomic analysis and phylogenomic analysis.
36282549	11	57	theme	EGI	1337:1339	arg1	L200015T					1341:1348	strain EGI L200015T	1330:1348	strain EGI L200015T	1330:1348	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	11	58	theme	genotypic	1297:1305	arg1	data					1324:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	the phenotypic, physiological, genotypic and phylogenetic data	1266:1327	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	4	59	theme	%	563:563	arg1	identity					547:554	sequence identity	538:554	sequence identity of 98.3%	538:563	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	11	60	dep	data	1324:1327	arg1	the					1253:1255	the	1253:1255	the	1253:1255	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	11	60	dep	data	1324:1327	arg1	basis					1257:1261	basis	1257:1261	basis	1257:1261	On the basis of the phenotypic, physiological, genotypic and phylogenetic data, strain EGI L200015T represents a novel species of the genus Pseudalkalibacillus, for which the name Pseudalkalibacillus salsuginis sp.
36282549	13	61	theme	proposed	1506:1513	arg1	isolate					1521:1527	the proposed novel isolate	1502:1527	the proposed novel isolate	1502:1527	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	5	62	theme	novel	570:574	arg1	isolate					576:582	The novel isolate	566:582	The novel isolate	566:582	The novel isolate could be distinguished from species of the genus Pseudalkalibacillus by its distinct phenotypic, physiological and genotypic characteristics.
36282549	10	63	dep	data	1196:1199	arg1	basis					1167:1171	basis	1167:1171	basis	1167:1171	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	63	dep	data	1196:1199	arg1	the					1163:1165	the	1163:1165	the	1163:1165	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	64	theme	G+C	1210:1212	arg1	%					1247:1247	41.6 %	1242:1247	41.6 %	1242:1247	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	64	theme	G+C	1210:1212	arg1	content					1214:1220	the DNA G+C content	1202:1220	the DNA G+C content of EGI L200015T	1202:1236	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	64	theme	G+C	1210:1212	arg1	data					1196:1199	the genome sequence data	1176:1199	the genome sequence data	1176:1199	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	7	65	theme	growth	818:823	arg1	conditions					825:834	Optimal growth conditions	810:834	Optimal growth conditions for EGI L200015T	810:851	Optimal growth conditions for EGI L200015T occurred on marine agar 2216 at pH 8.0 at 30 °C.
36282549	13	66	theme	CGMCC	1562:1566	arg1	1.19260T					1568:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	66	theme	CGMCC	1562:1566	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	2	67	theme	lake	195:198	arg1	sediment					200:207	saline lake sediment	188:207	saline lake sediment in Xinjiang Uygur Autonomous Region, PR China	188:253	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	4	68	dep	analysis	385:392	arg1	similarities					421:432	gene sequence similarities	407:432	gene sequence similarities	407:432	Phylogenetic analysis and 16S rRNA gene sequence similarities indicated that EGI L200015T formed a distinct clade with Pseudalkalibacillus berkeleyi KCTC 12718T with sequence identity of 98.3%.
36282549	13	69	theme	KCTC	1548:1551	arg1	1.19260T					1568:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	69	theme	KCTC	1548:1551	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	2	70	theme	saline	188:193	arg1	lake					195:198	saline lake	188:198	saline lake sediment in Xinjiang Uygur Autonomous Region, PR China	188:253	A salt-tolerant bacterium, designated strain EGI L200015T, was isolated from saline lake sediment in Xinjiang Uygur Autonomous Region, PR China.
36282549	9	71	theme	detected	1051:1058	arg1	lipids					1066:1071	The detected polar lipids	1047:1071	The detected polar lipids of	1047:1074	The detected polar lipids of included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
36282549	3	72	theme	polyphasic	315:324	arg1	analysis					336:343	polyphasic taxonomic analysis	315:343	polyphasic taxonomic analysis	315:343	The taxonomic position of the isolate was determined using polyphasic taxonomic analysis and phylogenomic analysis.
36282549	8	73	theme	fatty	958:962	arg1	acids					964:968	the major fatty acids	948:968	the major fatty acids (> 10 %)	948:977	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	73	theme	fatty	958:962	arg1	%					976:976	> 10 %	971:976	> 10 %	971:976	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	8	73	theme	fatty	958:962	arg1	anteiso-C15					984:994	anteiso-C15	984:994	anteiso-C15	984:994	The major respiratory quinone was MK-7, while the major fatty acids (> 10 %) were anteiso-C15 : 0, iso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
36282549	10	74	theme	DNA	1206:1208	arg1	%					1247:1247	41.6 %	1242:1247	41.6 %	1242:1247	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	74	theme	DNA	1206:1208	arg1	content					1214:1220	the DNA G+C content	1202:1220	the DNA G+C content of EGI L200015T	1202:1236	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	74	theme	DNA	1206:1208	arg1	data					1196:1199	the genome sequence data	1176:1199	the genome sequence data	1176:1199	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	3	75	theme	taxonomic	326:334	arg1	analysis					336:343	polyphasic taxonomic analysis	315:343	polyphasic taxonomic analysis	315:343	The taxonomic position of the isolate was determined using polyphasic taxonomic analysis and phylogenomic analysis.
36282549	1	76	theme	novel	43:47	arg1	bacterium					63:71	a novel salt-tolerant bacterium	41:71	a novel salt-tolerant bacterium	41:71	nov., a novel salt-tolerant bacterium isolated from a saline lake sediment.
36282549	1	76	theme	novel	43:47	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel salt-tolerant bacterium isolated from a saline lake sediment.
36282549	13	77	theme	=	1560:1560	arg1	1.19260T					1568:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	= KCTC 43363T = CGMCC 1.19260T	1546:1575	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	77	theme	=	1560:1560	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	78	theme	isolate	1521:1527	arg1	L200015T					1536:1543	EGI L200015T	1532:1543	EGI L200015T (= KCTC 43363T = CGMCC 1.19260T)	1532:1576	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	13	78	theme	isolate	1521:1527	arg1	strain					1492:1497	The type strain	1483:1497	The type strain of the proposed novel isolate	1483:1527	The type strain of the proposed novel isolate is EGI L200015T (= KCTC 43363T = CGMCC 1.19260T).
36282549	10	79	theme	L200015T	1229:1236	arg1	%					1247:1247	41.6 %	1242:1247	41.6 %	1242:1247	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	79	theme	L200015T	1229:1236	arg1	content					1214:1220	the DNA G+C content	1202:1220	the DNA G+C content of EGI L200015T	1202:1236	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36282549	10	79	theme	L200015T	1229:1236	arg1	data					1196:1199	the genome sequence data	1176:1199	the genome sequence data	1176:1199	On the basis of the genome sequence data, the DNA G+C content of EGI L200015T was 41.6 %.
36132991	7	0	theme	microbiome	1059:1068	arg1	roles					1042:1046	the roles	1038:1046	the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice	1038:1129	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	2	1	theme	A	385:385	arg1	effects					327:333	the protective effects	312:333	the protective effects	312:333	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	2	1	theme	A	385:385	arg1	mechanism					358:366	possible molecular mechanism	339:366	possible molecular mechanism	339:366	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	1	2	theme	barrier	266:272	arg1	disruption					274:283	barrier disruption	266:283	barrier disruption	266:283	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	5	3	used	used	795:798	arg2	inhibitor					776:784	TLR4 inhibitor	771:784	TLR4 inhibitor	771:784	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	10	4	from	composition	1550:1560	arg1	mice					1589:1592	NAFLD mice	1583:1592	NAFLD mice	1583:1592	Sin A treatment altered the composition of the microbiome in NAFLD mice compared to vehicle controls.
36132991	1	5	attach	linked	171:176	arg2	NAFLD					149:153	NAFLD	149:153	NAFLD	149:153	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	1	5	attach	linked	171:176	arg1	dysregulation					181:193	dysregulation	181:193	dysregulation of the gut-liver axis	181:215	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	1	5	attach	linked	171:176	arg2	disease					140:146	Background Non-alcoholic fatty liver disease	103:146	Background Non-alcoholic fatty liver disease (NAFLD)	103:154	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	9	6	theme	lipopolysaccharide	1381:1398	arg1	release					1370:1376	the release	1366:1376	the release of lipopolysaccharide (LPS) into circulation	1366:1421	We also observed that Sin A improved gut permeability and reduced the release of lipopolysaccharide (LPS) into circulation and further found that Sin A can suppress LPS-TLR4 signaling to protect against HFD-induced NAFLD.
36132991	8	7	theme	HFD-induced	1181:1191	arg1	steatosis					1201:1209	HFD-induced hepatic steatosis	1181:1209	HFD-induced hepatic steatosis	1181:1209	Results We found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function.
36132991	3	8	dep	vehicle	496:502	arg1	the					492:494	the	492:494	the	492:494	Methods HFD-fed NAFLD mice were treated with the vehicle and 80 mg/kg Sin A every day for 6 weeks.
36132991	11	9	theme	gut	1739:1741	arg1	inflammation					1743:1754	gut inflammation	1739:1754	gut inflammation	1739:1754	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	2	10	theme	protective	316:325	arg1	effects					327:333	the protective effects	312:333	the protective effects	312:333	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	1	11	theme	axis	212:215	arg1	dysregulation					181:193	dysregulation	181:193	dysregulation of the gut-liver axis	181:215	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	4	12	theme	intestinal	601:610	arg1	assays					625:630	intestinal permeability assays	601:630	intestinal permeability assays	601:630	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	7	13	theme	treatment	1001:1009	arg1	experiment					976:985	the experiment	972:985	the experiment of antibiotic treatment	972:1009	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	0	14	theme	non-alcoholic	27:39	arg1	disease					53:59	non-alcoholic fatty liver disease	27:59	non-alcoholic fatty liver disease	27:59	Schisantherin A alleviates non-alcoholic fatty liver disease by restoring intestinal barrier function.
36132991	5	15	theme	TLR4	771:774	arg1	inhibitor					776:784	TLR4 inhibitor	771:784	TLR4 inhibitor	771:784	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	8	16	theme	Sin	1154:1156	arg1	A					1158:1158	Sin A	1154:1158	Sin A	1154:1158	Results We found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function.
36132991	9	17	dep	improved	1328:1335	arg1	A					1326:1326	A	1326:1326	A	1326:1326	We also observed that Sin A improved gut permeability and reduced the release of lipopolysaccharide (LPS) into circulation and further found that Sin A can suppress LPS-TLR4 signaling to protect against HFD-induced NAFLD.
36132991	11	18	theme	intestinal	1767:1776	arg1	function					1786:1793	intestinal barrier function	1767:1793	intestinal barrier function	1767:1793	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	5	19	theme	TLR4	847:850	arg1	pathway					862:868	the LPS- TLR4 signaling pathway	838:868	the LPS- TLR4 signaling pathway	838:868	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	5	20	theme	LPS-	842:845	arg1	pathway					862:868	the LPS- TLR4 signaling pathway	838:868	the LPS- TLR4 signaling pathway	838:868	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	5	21	from	impact	819:824	arg1	pathway					862:868	the LPS- TLR4 signaling pathway	838:868	the LPS- TLR4 signaling pathway	838:868	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	9	22	theme	Sin	1446:1448	arg1	A					1450:1450	Sin A	1446:1450	Sin A	1446:1450	We also observed that Sin A improved gut permeability and reduced the release of lipopolysaccharide (LPS) into circulation and further found that Sin A can suppress LPS-TLR4 signaling to protect against HFD-induced NAFLD.
36132991	4	23	theme	transepithelial	644:658	arg1	TEER					683:686	TEER	683:686	TEER	683:686	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	4	23	theme	transepithelial	644:658	arg1	resistance					671:680	transepithelial electrical resistance	644:680	transepithelial electrical resistance (TEER) measurements in vitro	644:709	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	11	24	theme	hepatoprotective	1667:1682	arg1	A					1640:1640	Conclusions Sin A	1624:1640	Conclusions Sin A	1624:1640	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	11	24	theme	hepatoprotective	1667:1682	arg1	agent					1684:1688	an effective and safe hepatoprotective agent	1645:1688	an effective and safe hepatoprotective agent against HFD-induced NAFLD	1645:1714	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	5	25	theme	A	833:833	arg1	impact					819:824	the impact	815:824	the impact of Sin A in the LPS- TLR4 signaling pathway	815:868	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	2	26	from	effects	327:333	arg1	HFD					415:417	HFD	415:417	HFD	415:417	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	2	26	from	effects	327:333	arg1	diet					409:412	a high-fat diet	398:412	a high-fat diet (HFD) induced NAFLD mouse model	398:444	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	6	27	theme	rRNA	943:946	arg1	amplification					948:960	16S rRNA amplification	939:960	16S rRNA amplification	939:960	Alternatively, the composition of the microbiome was assessed using 16S rRNA amplification.
36132991	8	28	theme	intestinal	1271:1280	arg1	function					1290:1297	intestinal barrier function	1271:1297	intestinal barrier function	1271:1297	Results We found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function.
36132991	0	29	theme	barrier	85:91	arg1	function					93:100	intestinal barrier function	74:100	intestinal barrier function	74:100	Schisantherin A alleviates non-alcoholic fatty liver disease by restoring intestinal barrier function.
36132991	9	30	theme	LPS-TLR4	1465:1472	arg1	signaling					1474:1482	LPS-TLR4 signaling	1465:1482	LPS-TLR4 signaling	1465:1482	We also observed that Sin A improved gut permeability and reduced the release of lipopolysaccharide (LPS) into circulation and further found that Sin A can suppress LPS-TLR4 signaling to protect against HFD-induced NAFLD.
36132991	4	31	dep	in	702:703	arg1	vitro					705:709	vitro	705:709	vitro	705:709	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	9	32	theme	gut	1337:1339	arg1	permeability					1341:1352	gut permeability	1337:1352	gut permeability	1337:1352	We also observed that Sin A improved gut permeability and reduced the release of lipopolysaccharide (LPS) into circulation and further found that Sin A can suppress LPS-TLR4 signaling to protect against HFD-induced NAFLD.
36132991	2	33	theme	high-fat	400:407	arg1	HFD					415:417	HFD	415:417	HFD	415:417	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	2	33	theme	high-fat	400:407	arg1	diet					409:412	a high-fat diet	398:412	a high-fat diet (HFD) induced NAFLD mouse model	398:444	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	10	34	theme	NAFLD	1583:1587	arg1	mice					1589:1592	NAFLD mice	1583:1592	NAFLD mice	1583:1592	Sin A treatment altered the composition of the microbiome in NAFLD mice compared to vehicle controls.
36132991	0	35	theme	Schisantherin	0:12	arg1	A					14:14	Schisantherin A	0:14	Schisantherin A	0:14	Schisantherin A alleviates non-alcoholic fatty liver disease by restoring intestinal barrier function.
36132991	1	36	theme	Background	103:112	arg1	NAFLD					149:153	NAFLD	149:153	NAFLD	149:153	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	1	36	theme	Background	103:112	arg1	disease					140:146	Background Non-alcoholic fatty liver disease	103:146	Background Non-alcoholic fatty liver disease (NAFLD)	103:154	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	2	37	theme	NAFLD	428:432	arg1	model					440:444	induced NAFLD mouse model	420:444	a high-fat diet (HFD) induced NAFLD mouse model	398:444	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	3	38	theme	HFD-fed	455:461	arg1	mice					469:472	HFD-fed NAFLD mice	455:472	HFD-fed NAFLD mice	455:472	Methods HFD-fed NAFLD mice were treated with the vehicle and 80 mg/kg Sin A every day for 6 weeks.
36132991	1	39	theme	fatty	128:132	arg1	NAFLD					149:153	NAFLD	149:153	NAFLD	149:153	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	1	39	theme	fatty	128:132	arg1	disease					140:146	Background Non-alcoholic fatty liver disease	103:146	Background Non-alcoholic fatty liver disease (NAFLD)	103:154	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	7	40	from	roles	1042:1046	arg1	mice					1126:1129	the NAFLD mice	1116:1129	the NAFLD mice	1116:1129	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	10	41	theme	vehicle	1606:1612	arg1	controls					1614:1621	vehicle controls	1606:1621	vehicle controls	1606:1621	Sin A treatment altered the composition of the microbiome in NAFLD mice compared to vehicle controls.
36132991	7	42	theme	induced	1086:1092	arg1	benefits					1104:1111	Sin A induced metabolic benefits	1080:1111	Sin A induced metabolic benefits	1080:1111	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	0	43	theme	fatty	41:45	arg1	disease					53:59	non-alcoholic fatty liver disease	27:59	non-alcoholic fatty liver disease	27:59	Schisantherin A alleviates non-alcoholic fatty liver disease by restoring intestinal barrier function.
36132991	8	44	dep	Results	1132:1138	arg1	found					1143:1147	found	1143:1147	found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function	1143:1297	Results We found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function.
36132991	11	45	theme	Sin	1636:1638	arg1	A					1640:1640	Conclusions Sin A	1624:1640	Conclusions Sin A	1624:1640	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	11	45	theme	Sin	1636:1638	arg1	agent					1684:1688	an effective and safe hepatoprotective agent	1645:1688	an effective and safe hepatoprotective agent against HFD-induced NAFLD	1645:1714	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	2	46	theme	Schisantherin	371:383	arg1	A					385:385	Schisantherin A	371:385	Schisantherin A (Sin A)	371:393	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	2	46	theme	Schisantherin	371:383	arg1	A					392:392	Sin A	388:392	Sin A	388:392	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	11	47	theme	safe	1662:1665	arg1	A					1640:1640	Conclusions Sin A	1624:1640	Conclusions Sin A	1624:1640	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	11	47	theme	safe	1662:1665	arg1	agent					1684:1688	an effective and safe hepatoprotective agent	1645:1688	an effective and safe hepatoprotective agent against HFD-induced NAFLD	1645:1714	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	2	48	dep	diet	409:412	arg1	model					440:444	induced NAFLD mouse model	420:444	a high-fat diet (HFD) induced NAFLD mouse model	398:444	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	3	49	dep	Methods	447:453	arg1	treated					479:485	treated	479:485	were treated with the vehicle and 80 mg/kg Sin A every day for 6 weeks	474:543	Methods HFD-fed NAFLD mice were treated with the vehicle and 80 mg/kg Sin A every day for 6 weeks.
36132991	11	50	theme	effective	1648:1656	arg1	A					1640:1640	Conclusions Sin A	1624:1640	Conclusions Sin A	1624:1640	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	11	50	theme	effective	1648:1656	arg1	agent					1684:1688	an effective and safe hepatoprotective agent	1645:1688	an effective and safe hepatoprotective agent against HFD-induced NAFLD	1645:1714	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	4	51	theme	gut	758:760	arg1	barrier					762:768	the gut barrier	754:768	the gut barrier	754:768	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	1	52	theme	intestinal	238:247	arg1	inflammation					249:260	intestinal inflammation	238:260	intestinal inflammation	238:260	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	4	53	theme	NAFLD	574:578	arg1	mice					580:583	the NAFLD mice	570:583	the NAFLD mice	570:583	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	7	54	theme	gut	1055:1057	arg1	microbiome					1059:1068	the gut microbiome	1051:1068	the gut microbiome mediating Sin A induced metabolic benefits	1051:1111	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	2	55	from	mechanism	358:366	arg1	HFD					415:417	HFD	415:417	HFD	415:417	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	2	55	from	mechanism	358:366	arg1	diet					409:412	a high-fat diet	398:412	a high-fat diet (HFD) induced NAFLD mouse model	398:444	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	10	56	theme	Sin	1522:1524	arg1	treatment					1528:1536	Sin A treatment	1522:1536	Sin A treatment	1522:1536	Sin A treatment altered the composition of the microbiome in NAFLD mice compared to vehicle controls.
36132991	4	57	theme	resistance	671:680	arg1	measurements					689:700	transepithelial electrical resistance (TEER) measurements	644:700	transepithelial electrical resistance (TEER) measurements in vitro	644:709	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	4	58	theme	gut	550:552	arg1	permeability					554:565	The gut permeability	546:565	The gut permeability of the NAFLD mice	546:583	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	11	59	theme	intestinal	1811:1820	arg1	composition					1833:1843	intestinal microbiota composition	1811:1843	intestinal microbiota composition	1811:1843	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	3	60	theme	mg/kg	511:515	arg1	Sin					517:519	80 mg/kg Sin	508:519	80 mg/kg Sin	508:519	Methods HFD-fed NAFLD mice were treated with the vehicle and 80 mg/kg Sin A every day for 6 weeks.
36132991	4	61	theme	permeability	612:623	arg1	assays					625:630	intestinal permeability assays	601:630	intestinal permeability assays	601:630	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	2	62	theme	possible	339:346	arg1	mechanism					358:366	possible molecular mechanism	339:366	possible molecular mechanism	339:366	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	6	63	theme	microbiome	909:918	arg1	composition					890:900	the composition	886:900	the composition of the microbiome	886:918	Alternatively, the composition of the microbiome was assessed using 16S rRNA amplification.
36132991	1	64	theme	gut-liver	202:210	arg1	axis					212:215	the gut-liver axis	198:215	the gut-liver axis	198:215	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	8	65	theme	hepatic	1193:1199	arg1	steatosis					1201:1209	HFD-induced hepatic steatosis	1181:1209	HFD-induced hepatic steatosis	1181:1209	Results We found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function.
36132991	10	66	theme	microbiome	1569:1578	arg1	composition					1550:1560	the composition	1546:1560	the composition of the microbiome in NAFLD mice	1546:1592	Sin A treatment altered the composition of the microbiome in NAFLD mice compared to vehicle controls.
36132991	7	67	theme	antibiotic	990:999	arg1	treatment					1001:1009	antibiotic treatment	990:1009	antibiotic treatment	990:1009	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	1	68	theme	liver	134:138	arg1	NAFLD					149:153	NAFLD	149:153	NAFLD	149:153	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	1	68	theme	liver	134:138	arg1	disease					140:146	Background Non-alcoholic fatty liver disease	103:146	Background Non-alcoholic fatty liver disease (NAFLD)	103:154	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	11	69	theme	barrier	1778:1784	arg1	function					1786:1793	intestinal barrier function	1767:1793	intestinal barrier function	1767:1793	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	5	70	theme	signaling	852:860	arg1	pathway					862:868	the LPS- TLR4 signaling pathway	838:868	the LPS- TLR4 signaling pathway	838:868	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	8	71	theme	gut	1240:1242	arg1	inflammation					1244:1255	gut inflammation	1240:1255	gut inflammation	1240:1255	Results We found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function.
36132991	11	72	theme	HFD-induced	1698:1708	arg1	NAFLD					1710:1714	HFD-induced NAFLD	1698:1714	HFD-induced NAFLD	1698:1714	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	0	73	theme	intestinal	74:83	arg1	function					93:100	intestinal barrier function	74:100	intestinal barrier function	74:100	Schisantherin A alleviates non-alcoholic fatty liver disease by restoring intestinal barrier function.
36132991	5	74	theme	Sin	829:831	arg1	A					833:833	Sin A	829:833	Sin A	829:833	TLR4 inhibitor was then used to investigate the impact of Sin A in the LPS- TLR4 signaling pathway.
36132991	10	75	theme	A	1526:1526	arg1	treatment					1528:1536	Sin A treatment	1522:1536	Sin A treatment	1522:1536	Sin A treatment altered the composition of the microbiome in NAFLD mice compared to vehicle controls.
36132991	6	76	theme	16S	939:941	arg1	amplification					948:960	16S rRNA amplification	939:960	16S rRNA amplification	939:960	Alternatively, the composition of the microbiome was assessed using 16S rRNA amplification.
36132991	4	77	theme	electrical	660:669	arg1	TEER					683:686	TEER	683:686	TEER	683:686	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	4	77	theme	electrical	660:669	arg1	resistance					671:680	transepithelial electrical resistance	644:680	transepithelial electrical resistance (TEER) measurements in vitro	644:709	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	8	78	theme	barrier	1282:1288	arg1	function					1290:1297	intestinal barrier function	1271:1297	intestinal barrier function	1271:1297	Results We found that Sin A potently ameliorated HFD-induced hepatic steatosis and inflammation, alleviated gut inflammation, and restored intestinal barrier function.
36132991	7	79	theme	NAFLD	1120:1124	arg1	mice					1126:1129	the NAFLD mice	1116:1129	the NAFLD mice	1116:1129	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	9	80	theme	HFD-induced	1503:1513	arg1	NAFLD					1515:1519	HFD-induced NAFLD	1503:1519	HFD-induced NAFLD	1503:1519	We also observed that Sin A improved gut permeability and reduced the release of lipopolysaccharide (LPS) into circulation and further found that Sin A can suppress LPS-TLR4 signaling to protect against HFD-induced NAFLD.
36132991	2	81	theme	mouse	434:438	arg1	model					440:444	induced NAFLD mouse model	420:444	a high-fat diet (HFD) induced NAFLD mouse model	398:444	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	4	82	theme	in	702:703	arg1	measurements					689:700	transepithelial electrical resistance (TEER) measurements	644:700	transepithelial electrical resistance (TEER) measurements in vitro	644:709	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	7	83	theme	metabolic	1094:1102	arg1	benefits					1104:1111	Sin A induced metabolic benefits	1080:1111	Sin A induced metabolic benefits	1080:1111	Finally, the experiment of antibiotic treatment was performed to elucidate the roles of the gut microbiome mediating Sin A induced metabolic benefits in the NAFLD mice.
36132991	1	84	theme	Non-alcoholic	114:126	arg1	NAFLD					149:153	NAFLD	149:153	NAFLD	149:153	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	1	84	theme	Non-alcoholic	114:126	arg1	disease					140:146	Background Non-alcoholic fatty liver disease	103:146	Background Non-alcoholic fatty liver disease (NAFLD)	103:154	Background Non-alcoholic fatty liver disease (NAFLD) is intricately linked to dysregulation of the gut-liver axis, and correlated with intestinal inflammation and barrier disruption.
36132991	2	85	theme	induced	420:426	arg1	model					440:444	induced NAFLD mouse model	420:444	a high-fat diet (HFD) induced NAFLD mouse model	398:444	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	3	86	theme	NAFLD	463:467	arg1	mice					469:472	HFD-fed NAFLD mice	455:472	HFD-fed NAFLD mice	455:472	Methods HFD-fed NAFLD mice were treated with the vehicle and 80 mg/kg Sin A every day for 6 weeks.
36132991	0	87	theme	liver	47:51	arg1	disease					53:59	non-alcoholic fatty liver disease	27:59	non-alcoholic fatty liver disease	27:59	Schisantherin A alleviates non-alcoholic fatty liver disease by restoring intestinal barrier function.
36132991	11	88	theme	Conclusions	1624:1634	arg1	A					1640:1640	Conclusions Sin A	1624:1640	Conclusions Sin A	1624:1640	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	11	88	theme	Conclusions	1624:1634	arg1	agent					1684:1688	an effective and safe hepatoprotective agent	1645:1688	an effective and safe hepatoprotective agent against HFD-induced NAFLD	1645:1714	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	4	89	theme	barrier	762:768	arg1	function					742:749	the function	738:749	the function of the gut barrier	738:768	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	2	90	theme	Sin	388:390	arg1	A					385:385	Schisantherin A	371:385	Schisantherin A (Sin A)	371:393	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	2	90	theme	Sin	388:390	arg1	A					392:392	Sin A	388:392	Sin A	388:392	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	11	91	theme	microbiota	1822:1831	arg1	composition					1833:1843	intestinal microbiota composition	1811:1843	intestinal microbiota composition	1811:1843	Conclusions Sin A is an effective and safe hepatoprotective agent against HFD-induced NAFLD by partly ameliorating gut inflammation, restoring intestinal barrier function, and regulating intestinal microbiota composition.
36132991	2	92	theme	molecular	348:356	arg1	mechanism					358:366	possible molecular mechanism	339:366	possible molecular mechanism	339:366	Objectives To investigate the protective effects and possible molecular mechanism of Schisantherin A (Sin A) in a high-fat diet (HFD) induced NAFLD mouse model.
36132991	4	93	used	used	721:724	arg2	measurements					689:700	transepithelial electrical resistance (TEER) measurements	644:700	transepithelial electrical resistance (TEER) measurements in vitro	644:709	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	4	94	theme	mice	580:583	arg1	permeability					554:565	The gut permeability	546:565	The gut permeability of the NAFLD mice	546:583	The gut permeability of the NAFLD mice was assessed by intestinal permeability assays in vivo and transepithelial electrical resistance (TEER) measurements in vitro were also used to evaluate the function of the gut barrier.
36132991	3	95	theme	80	508:509	arg1	mg/kg					511:515	mg/kg	511:515	mg/kg	511:515	Methods HFD-fed NAFLD mice were treated with the vehicle and 80 mg/kg Sin A every day for 6 weeks.
36613700	3	0	theme	protein	305:311	arg1	composition					313:323	the protein composition	301:323	the protein composition of sericin extracts	301:343	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	4	1	theme	Quantitative	496:507	arg1	qRT-PCR					524:530	qRT-PCR	524:530	qRT-PCR	524:530	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	4	1	theme	Quantitative	496:507	arg1	PCR					519:521	Quantitative Real-time PCR	496:521	Quantitative Real-time PCR (qRT-PCR)	496:531	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	7	2	theme	SS2	1112:1114	arg1	treatment					1116:1124	SS2 treatment	1112:1124	SS2 treatment	1112:1124	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	9	3	theme	MyD88/NF-κB	1361:1371	arg1	pathway					1373:1379	MyD88/NF-κB pathway	1361:1379	MyD88/NF-κB pathway	1361:1379	It was confirmed that sericin inhibited LPS-induced inflammation through MyD88/NF-κB pathway.
36613700	4	4	theme	anti-inflammation	564:580	arg1	ability					582:588	the anti-inflammation ability	560:588	the anti-inflammation ability of sericin	560:599	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	0	5	theme	Inflammation	82:93	arg1	Mechanism					38:46	the Mechanism	34:46	the Mechanism of Sericin Inhibiting LPS-Induced Inflammation	34:93	Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation.
36613700	7	6	theme	gene	1063:1066	arg1	NOD1					1068:1071	receptor gene NOD1	1054:1071	receptor gene NOD1	1054:1071	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	6	7	dep	receptors	940:948	arg1	pathways					997:1004	signaling pathways	987:1004	signaling pathways	987:1004	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	4	8	theme	Immunosorbent	464:476	arg1	ELISA					485:489	ELISA	485:489	ELISA	485:489	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	4	8	theme	Immunosorbent	464:476	arg1	Assay					478:482	Enzyme-linked Immunosorbent Assay	450:482	Enzyme-linked Immunosorbent Assay (ELISA)	450:490	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	8	9	theme	other	1258:1262	arg1	cytokines					1277:1285	other inflammatory cytokines	1258:1285	other inflammatory cytokines	1258:1285	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	5	10	theme	inhibitory	712:721	arg1	capacity					723:730	the inhibitory capacity	708:730	the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages	708:782	Finally, RNA-seq was performed to quantify the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages.
36613700	10	11	theme	theoretical	1414:1424	arg1	support					1426:1432	necessary theoretical support	1404:1432	necessary theoretical support for sericin development and application	1404:1472	This finding provides necessary theoretical support for sericin development and application.
36613700	10	12	theme	necessary	1404:1412	arg1	support					1426:1432	necessary theoretical support	1404:1432	necessary theoretical support for sericin development and application	1404:1472	This finding provides necessary theoretical support for sericin development and application.
36613700	3	13	theme	extracts	336:343	arg1	composition					313:323	the protein composition	301:323	the protein composition of sericin extracts	301:343	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	6	14	theme	functional	794:803	arg1	annotation					805:814	The gene functional annotation	785:814	The gene functional annotation	785:814	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	2	15	theme	protein	266:272	arg1	mechanism					245:253	the anti-inflammatory mechanism	223:253	the anti-inflammatory mechanism of sericin protein	223:272	In this study, the anti-inflammatory mechanism of sericin protein was investigated.
36613700	5	16	theme	sericin	735:741	arg1	SS2					750:752	sericin sample SS2	735:752	sericin sample SS2	735:752	Finally, RNA-seq was performed to quantify the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages.
36613700	6	17	theme	PRRs	854:857	arg1	receptors					940:948	the Toll-like receptors	926:948	the Toll-like receptors (TLRs)	926:955	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	6	17	theme	PRRs	854:857	arg1	receptors					970:978	NOD-like receptors	961:978	NOD-like receptors (NLRs)	961:985	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	6	17	theme	PRRs	854:857	arg1	pathways					869:876	almost all PRRs signaling pathways	843:876	almost all PRRs signaling pathways	843:876	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	2	18	theme	sericin	258:264	arg1	protein					266:272	sericin protein	258:272	sericin protein	258:272	In this study, the anti-inflammatory mechanism of sericin protein was investigated.
36613700	4	19	theme	sericin	646:652	arg1	protein					656:662	sericin 1 protein	646:662	sericin 1 protein	646:662	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	3	20	theme	Mass	392:395	arg1	LC-MS/MS					411:418	LC-MS/MS	411:418	LC-MS/MS	411:418	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	3	20	theme	Mass	392:395	arg1	Spectrometry					397:408	Liquid Chromatography-Tandem Mass Spectrometry	363:408	Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	363:419	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	5	21	theme	LPS-stimulated	757:770	arg1	macrophages					772:782	LPS-stimulated macrophages	757:782	LPS-stimulated macrophages	757:782	Finally, RNA-seq was performed to quantify the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages.
36613700	1	22	with	protein	117:123	arg1	potential					147:155	high application potential	130:155	high application potential	130:155	Sericin is a natural protein with high application potential, but the research on its efficacy is very limited.
36613700	5	23	theme	sample	743:748	arg1	SS2					750:752	sericin sample SS2	735:752	sericin sample SS2	735:752	Finally, RNA-seq was performed to quantify the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages.
36613700	10	24	theme	sericin	1438:1444	arg1	development					1446:1456	sericin development	1438:1456	sericin development	1438:1456	This finding provides necessary theoretical support for sericin development and application.
36613700	0	25	theme	Multi-Omics	0:10	arg1	Integration					12:22	Multi-Omics Integration	0:22	Multi-Omics Integration	0:22	Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation.
36613700	6	26	theme	NOD-like	961:968	arg1	NLRs					981:984	NLRs	981:984	NLRs	981:984	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	6	26	theme	NOD-like	961:968	arg1	receptors					970:978	NOD-like receptors	961:978	NOD-like receptors (NLRs)	961:985	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	7	27	theme	NOD1	1068:1071	arg1	level					1022:1026	The expression level	1007:1026	The expression level of adaptor gene MyD88 and receptor gene NOD1	1007:1071	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	9	28	theme	LPS-induced	1328:1338	arg1	inflammation					1340:1351	LPS-induced inflammation	1328:1351	LPS-induced inflammation	1328:1351	It was confirmed that sericin inhibited LPS-induced inflammation through MyD88/NF-κB pathway.
36613700	4	29	theme	protein	656:662	arg1	purity					636:641	the purity	632:641	the purity of sericin 1 protein	632:662	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	3	30	theme	Liquid	363:368	arg1	LC-MS/MS					411:418	LC-MS/MS	411:418	LC-MS/MS	411:418	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	3	30	theme	Liquid	363:368	arg1	Spectrometry					397:408	Liquid Chromatography-Tandem Mass Spectrometry	363:408	Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	363:419	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	8	31	theme	cytokines	1277:1285	arg1	expressions					1220:1230	the expressions	1216:1230	the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines	1216:1285	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	8	32	theme	IL-1β	1235:1239	arg1	expressions					1220:1230	the expressions	1216:1230	the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines	1216:1285	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	3	33	theme	Chromatography-Tandem	370:390	arg1	LC-MS/MS					411:418	LC-MS/MS	411:418	LC-MS/MS	411:418	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	3	33	theme	Chromatography-Tandem	370:390	arg1	Spectrometry					397:408	Liquid Chromatography-Tandem Mass Spectrometry	363:408	Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)	363:419	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	7	34	theme	receptor	1054:1061	arg1	NOD1					1068:1071	receptor gene NOD1	1054:1071	receptor gene NOD1	1054:1071	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	4	35	theme	Enzyme-linked	450:462	arg1	ELISA					485:489	ELISA	485:489	ELISA	485:489	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	4	35	theme	Enzyme-linked	450:462	arg1	Assay					478:482	Enzyme-linked Immunosorbent Assay	450:482	Enzyme-linked Immunosorbent Assay (ELISA)	450:490	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	1	36	theme	natural	109:115	arg1	protein					117:123	a natural protein	107:123	a natural protein with high application potential	107:155	Sericin is a natural protein with high application potential, but the research on its efficacy is very limited.
36613700	1	36	theme	natural	109:115	arg1	Sericin					96:102	Sericin	96:102	Sericin	96:102	Sericin is a natural protein with high application potential, but the research on its efficacy is very limited.
36613700	7	37	theme	gene	1039:1042	arg1	MyD88					1044:1048	adaptor gene MyD88	1031:1048	adaptor gene MyD88	1031:1048	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	8	38	theme	IL-6	1242:1245	arg1	expressions					1220:1230	the expressions	1216:1230	the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines	1216:1285	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	7	39	theme	MyD88	1044:1048	arg1	level					1022:1026	The expression level	1007:1026	The expression level of adaptor gene MyD88 and receptor gene NOD1	1007:1071	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	6	40	theme	signaling	859:867	arg1	receptors					940:948	the Toll-like receptors	926:948	the Toll-like receptors (TLRs)	926:955	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	6	40	theme	signaling	859:867	arg1	receptors					970:978	NOD-like receptors	961:978	NOD-like receptors (NLRs)	961:985	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	6	40	theme	signaling	859:867	arg1	pathways					869:876	almost all PRRs signaling pathways	843:876	almost all PRRs signaling pathways	843:876	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	8	41	theme	P38	1185:1187	arg1	levels					1164:1169	the phosphorylation levels	1144:1169	the phosphorylation levels of NF-κB P65, P38, and JNK	1144:1196	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	0	42	theme	Sericin	51:57	arg1	Inflammation					82:93	Sericin Inhibiting LPS-Induced Inflammation	51:93	Sericin Inhibiting LPS-Induced Inflammation	51:93	Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation.
36613700	8	43	theme	phosphorylation	1148:1162	arg1	levels					1164:1169	the phosphorylation levels	1144:1169	the phosphorylation levels of NF-κB P65, P38, and JNK	1144:1196	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	0	44	theme	Inhibiting	59:68	arg1	Inflammation					82:93	Sericin Inhibiting LPS-Induced Inflammation	51:93	Sericin Inhibiting LPS-Induced Inflammation	51:93	Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation.
36613700	8	45	theme	INOS	1248:1251	arg1	expressions					1220:1230	the expressions	1216:1230	the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines	1216:1285	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	8	46	theme	JNK	1194:1196	arg1	levels					1164:1169	the phosphorylation levels	1144:1169	the phosphorylation levels of NF-κB P65, P38, and JNK	1144:1196	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	6	47	theme	Toll-like	930:938	arg1	receptors					940:948	the Toll-like receptors	926:948	the Toll-like receptors (TLRs)	926:955	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	6	47	theme	Toll-like	930:938	arg1	TLRs					951:954	TLRs	951:954	TLRs	951:954	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	7	48	theme	adaptor	1031:1037	arg1	MyD88					1044:1048	adaptor gene MyD88	1031:1048	adaptor gene MyD88	1031:1048	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	7	49	theme	expression	1011:1020	arg1	level					1022:1026	The expression level	1007:1026	The expression level of adaptor gene MyD88 and receptor gene NOD1	1007:1071	The expression level of adaptor gene MyD88 and receptor gene NOD1 was significantly down-regulated after SS2 treatment.
36613700	6	50	theme	gene	789:792	arg1	annotation					805:814	The gene functional annotation	785:814	The gene functional annotation	785:814	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36613700	5	51	theme	SS2	750:752	arg1	capacity					723:730	the inhibitory capacity	708:730	the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages	708:782	Finally, RNA-seq was performed to quantify the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages.
36613700	1	52	theme	high	130:133	arg1	potential					147:155	high application potential	130:155	high application potential	130:155	Sericin is a natural protein with high application potential, but the research on its efficacy is very limited.
36613700	8	53	theme	NF-κB	1174:1178	arg1	P65					1180:1182	NF-κB P65	1174:1182	NF-κB P65	1174:1182	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	1	54	theme	application	135:145	arg1	potential					147:155	high application potential	130:155	high application potential	130:155	Sericin is a natural protein with high application potential, but the research on its efficacy is very limited.
36613700	4	55	theme	sericin	593:599	arg1	ability					582:588	the anti-inflammation ability	560:588	the anti-inflammation ability of sericin	560:599	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	0	56	theme	LPS-Induced	70:80	arg1	Inflammation					82:93	Sericin Inhibiting LPS-Induced Inflammation	51:93	Sericin Inhibiting LPS-Induced Inflammation	51:93	Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation.
36613700	2	57	theme	anti-inflammatory	227:243	arg1	mechanism					245:253	the anti-inflammatory mechanism	223:253	the anti-inflammatory mechanism of sericin protein	223:272	In this study, the anti-inflammatory mechanism of sericin protein was investigated.
36613700	8	58	theme	inflammatory	1264:1275	arg1	cytokines					1277:1285	other inflammatory cytokines	1258:1285	other inflammatory cytokines	1258:1285	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	5	59	from	capacity	723:730	arg1	macrophages					772:782	LPS-stimulated macrophages	757:782	LPS-stimulated macrophages	757:782	Finally, RNA-seq was performed to quantify the inhibitory capacity of sericin sample SS2 in LPS-stimulated macrophages.
36613700	4	60	link	Enzyme-linked	450:462	arg1	ELISA					485:489	ELISA	485:489	ELISA	485:489	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	4	60	link	Enzyme-linked	450:462	arg1	Assay					478:482	Enzyme-linked Immunosorbent Assay	450:482	Enzyme-linked Immunosorbent Assay (ELISA)	450:490	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	3	61	theme	sericin	328:334	arg1	extracts					336:343	sericin extracts	328:343	sericin extracts	328:343	Firstly, the protein composition of sericin extracts was determined by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
36613700	4	62	theme	Real-time	509:517	arg1	qRT-PCR					524:530	qRT-PCR	524:530	qRT-PCR	524:530	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	4	62	theme	Real-time	509:517	arg1	PCR					519:521	Quantitative Real-time PCR	496:521	Quantitative Real-time PCR (qRT-PCR)	496:531	This was then combined with Enzyme-linked Immunosorbent Assay (ELISA) and Quantitative Real-time PCR (qRT-PCR), and it was confirmed that the anti-inflammation ability of sericin was positively correlated with the purity of sericin 1 protein.
36613700	1	63	from	research	166:173	arg1	efficacy					182:189	its efficacy	178:189	its efficacy	178:189	Sericin is a natural protein with high application potential, but the research on its efficacy is very limited.
36613700	8	64	theme	P65	1180:1182	arg1	levels					1164:1169	the phosphorylation levels	1144:1169	the phosphorylation levels of NF-κB P65, P38, and JNK	1144:1196	SS2 also reduced the phosphorylation levels of NF-κB P65, P38, and JNK, thereby reducing the expressions of IL-1β, IL-6, INOS, and other inflammatory cytokines.
36613700	6	65	theme	signaling	987:995	arg1	pathways					997:1004	signaling pathways	987:1004	signaling pathways	987:1004	The gene functional annotation showed that SS2 suppressed almost all PRRs signaling pathways activated by lipopolysaccharides (LPS), such as the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) signaling pathways.
36144693	6	0	theme	extracts	515:522	arg1	composition					495:505	the chemical composition	482:505	the chemical composition of some extracts	482:522	Therefore, this study determined the chemical composition of some extracts and evaluated their cytotoxicity, along with their antioxidant and anti-inflammatory, activities using in vitro models.
36144693	12	1	theme	peroxidation	1289:1300	arg1	terms					1248:1252	terms	1248:1252	terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition	1248:1320	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	12	2	theme	significant	1197:1207	arg1	activity					1221:1228	significant antioxidant activity	1197:1228	significant antioxidant activity	1197:1228	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	5	3	theme	information	380:390	arg1	lack					372:375	a lack	370:375	a lack of information about the phytochemistry and bioactivity of M. albicans	370:446	There is a lack of information about the phytochemistry and bioactivity of M. albicans.
36144693	9	4	theme	tumoral	907:913	arg1	cells					923:927	tumoral hepatic cells	907:927	tumoral hepatic cells (Hep-G2)	907:936	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	9	4	theme	tumoral	907:913	arg1	Hep-G2					930:935	Hep-G2	930:935	Hep-G2	930:935	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	2	5	theme	Miconia	110:116	arg1	albicans					118:125	The plant Miconia albicans	100:125	The plant Miconia albicans (Sw.)	100:131	The plant Miconia albicans (Sw.)
36144693	13	6	theme	pro-inflammatory	1404:1419	arg1	cytokines					1421:1429	fewer pro-inflammatory cytokines	1398:1429	fewer pro-inflammatory cytokines	1398:1429	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS.
36144693	9	7	theme	hepatic	915:921	arg1	cells					923:927	tumoral hepatic cells	907:927	tumoral hepatic cells (Hep-G2)	907:936	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	9	7	theme	hepatic	915:921	arg1	Hep-G2					930:935	Hep-G2	930:935	Hep-G2	930:935	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	2	8	theme	plant	104:108	arg1	albicans					118:125	The plant Miconia albicans	100:125	The plant Miconia albicans (Sw.)	100:131	The plant Miconia albicans (Sw.)
36144693	3	9	theme	chronic	183:189	arg1	disturbances					204:215	chronic inflammatory disturbances	183:215	chronic inflammatory disturbances	183:215	Triana has been popularly used in Brazil to treat chronic inflammatory disturbances, such as osteoarthritis.
36144693	3	9	theme	chronic	183:189	arg1	osteoarthritis					226:239	osteoarthritis	226:239	osteoarthritis	226:239	Triana has been popularly used in Brazil to treat chronic inflammatory disturbances, such as osteoarthritis.
36144693	14	10	theme	new	1600:1602	arg1	drugs					1604:1608	new drugs	1600:1608	new drugs	1600:1608	In the future, it will be necessary to identify the phytochemicals that are responsible for anti-inflammatory effects for the discovery of new drugs.
36144693	3	11	theme	inflammatory	191:202	arg1	disturbances					204:215	chronic inflammatory disturbances	183:215	chronic inflammatory disturbances	183:215	Triana has been popularly used in Brazil to treat chronic inflammatory disturbances, such as osteoarthritis.
36144693	3	11	theme	inflammatory	191:202	arg1	osteoarthritis					226:239	osteoarthritis	226:239	osteoarthritis	226:239	Triana has been popularly used in Brazil to treat chronic inflammatory disturbances, such as osteoarthritis.
36144693	5	12	dep	phytochemistry	402:415	arg1	the					398:400	the	398:400	the	398:400	There is a lack of information about the phytochemistry and bioactivity of M. albicans.
36144693	13	13	theme	fewer	1398:1402	arg1	cytokines					1421:1429	fewer pro-inflammatory cytokines	1398:1429	fewer pro-inflammatory cytokines	1398:1429	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS.
36144693	4	14	theme	people	275:280	arg1	worldwide					282:290	250 million people worldwide	263:290	250 million people worldwide	263:290	This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function.
36144693	6	15	theme	chemical	486:493	arg1	composition					495:505	the chemical composition	482:505	the chemical composition of some extracts	482:522	Therefore, this study determined the chemical composition of some extracts and evaluated their cytotoxicity, along with their antioxidant and anti-inflammatory, activities using in vitro models.
36144693	15	16	theme	possible	1687:1694	arg1	mechanisms					1696:1705	their possible mechanisms	1681:1705	their possible mechanisms of action	1681:1715	In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
36144693	4	17	theme	million	267:273	arg1	worldwide					282:290	250 million people worldwide	263:290	250 million people worldwide	263:290	This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function.
36144693	12	18	theme	activity	1273:1280	arg1	terms					1248:1252	terms	1248:1252	terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition	1248:1320	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	4	19	theme	articular	341:349	arg1	function					351:358	articular function	341:358	articular function	341:358	This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function.
36144693	3	20	used	used	159:162	arg2	Triana					133:138	Triana	133:138	Triana	133:138	Triana has been popularly used in Brazil to treat chronic inflammatory disturbances, such as osteoarthritis.
36144693	12	21	theme	scavenging	1262:1271	arg1	activity					1273:1280	DPPH scavenging activity	1257:1280	DPPH scavenging activity	1257:1280	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	10	22	from	flavonoids	996:1005	arg1	fractions					1014:1022	all fractions	1010:1022	all fractions	1010:1022	LC-MS analyses identified several flavonoids in all fractions, such as quercetin, myricetin, and their glycosides.
36144693	14	23	theme	drugs	1604:1608	arg1	discovery					1587:1595	the discovery	1583:1595	the discovery of new drugs	1583:1608	In the future, it will be necessary to identify the phytochemicals that are responsible for anti-inflammatory effects for the discovery of new drugs.
36144693	15	24	theme	albicans	1633:1640	arg1	extracts					1642:1649	M. albicans extracts	1630:1649	M. albicans extracts	1630:1649	In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
36144693	12	25	theme	lipid	1283:1287	arg1	peroxidation					1289:1300	lipid peroxidation	1283:1300	lipid peroxidation	1283:1300	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	0	26	theme	Phytochemical	0:12	arg1	Evaluation					14:23	Phytochemical Evaluation	0:23	Phytochemical Evaluation	0:23	Phytochemical Evaluation and Anti-Inflammatory Potential of Miconia albicans (Sw.)
36144693	0	27	theme	Anti-Inflammatory	29:45	arg1	Potential					47:55	Anti-Inflammatory Potential	29:55	Anti-Inflammatory Potential	29:55	Phytochemical Evaluation and Anti-Inflammatory Potential of Miconia albicans (Sw.)
36144693	14	28	from	necessary	1487:1495	arg1	future					1468:1473	future	1468:1473	future	1468:1473	In the future, it will be necessary to identify the phytochemicals that are responsible for anti-inflammatory effects for the discovery of new drugs.
36144693	9	29	theme	biological	842:851	arg1	activities					853:862	their biological activities	836:862	their biological activities	836:862	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	4	30	theme	function	351:358	arg1	pain					324:327	intense pain	316:327	intense pain	316:327	This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function.
36144693	4	30	theme	function	351:358	arg1	loss					333:336	loss	333:336	loss of articular function	333:358	This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function.
36144693	5	31	theme	albicans	439:446	arg1	bioactivity					421:431	bioactivity	421:431	bioactivity	421:431	There is a lack of information about the phytochemistry and bioactivity of M. albicans.
36144693	5	31	theme	albicans	439:446	arg1	phytochemistry					402:415	phytochemistry	402:415	phytochemistry	402:415	There is a lack of information about the phytochemistry and bioactivity of M. albicans.
36144693	9	32	theme	THP-1	943:947	arg1	macrophages					949:959	THP-1 macrophages	943:959	THP-1 macrophages	943:959	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	4	33	theme	250	263:265	arg1	million					267:273	million	267:273	million	267:273	This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function.
36144693	8	34	theme	liquid-liquid	704:716	arg1	partition					718:726	a liquid-liquid partition	702:726	a liquid-liquid partition	702:726	Afterwards, a liquid-liquid partition was developed using chloroform, ethyl acetate, and n-butanol.
36144693	11	35	theme	crude	1081:1085	arg1	extracts					1087:1094	The crude extracts	1077:1094	The crude extracts	1077:1094	The crude extracts and n-butanol fractions did not present cytotoxicity to the cells.
36144693	12	36	theme	DPPH	1257:1260	arg1	activity					1273:1280	DPPH scavenging activity	1257:1280	DPPH scavenging activity	1257:1280	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	10	37	theme	several	988:994	arg1	flavonoids					996:1005	several flavonoids	988:1005	several flavonoids	988:1005	LC-MS analyses identified several flavonoids in all fractions, such as quercetin, myricetin, and their glycosides.
36144693	10	37	theme	several	988:994	arg1	myricetin					1044:1052	myricetin	1044:1052	myricetin	1044:1052	LC-MS analyses identified several flavonoids in all fractions, such as quercetin, myricetin, and their glycosides.
36144693	10	37	theme	several	988:994	arg1	quercetin					1033:1041	quercetin	1033:1041	quercetin	1033:1041	LC-MS analyses identified several flavonoids in all fractions, such as quercetin, myricetin, and their glycosides.
36144693	10	37	theme	several	988:994	arg1	glycosides					1065:1074	their glycosides	1059:1074	their glycosides	1059:1074	LC-MS analyses identified several flavonoids in all fractions, such as quercetin, myricetin, and their glycosides.
36144693	12	38	theme	inhibition	1311:1320	arg1	terms					1248:1252	terms	1248:1252	terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition	1248:1320	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	15	39	theme	In	1611:1612	arg1	studies					1619:1625	In vivo studies	1611:1625	In vivo studies on M. albicans extracts	1611:1649	In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
36144693	13	40	theme	n-butanol	1358:1366	arg1	fraction					1368:1375	the n-butanol fraction	1354:1375	the n-butanol fraction (250 µg/mL)	1354:1387	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS.
36144693	13	40	theme	n-butanol	1358:1366	arg1	µg/mL					1382:1386	250 µg/mL	1378:1386	250 µg/mL	1378:1386	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS.
36144693	0	41	theme	albicans	68:75	arg1	Evaluation					14:23	Phytochemical Evaluation	0:23	Phytochemical Evaluation	0:23	Phytochemical Evaluation and Anti-Inflammatory Potential of Miconia albicans (Sw.)
36144693	0	41	theme	albicans	68:75	arg1	Potential					47:55	Anti-Inflammatory Potential	29:55	Anti-Inflammatory Potential	29:55	Phytochemical Evaluation and Anti-Inflammatory Potential of Miconia albicans (Sw.)
36144693	13	42	dep	LPS	1456:1458	arg1	the					1440:1442	the	1440:1442	the	1440:1442	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS.
36144693	13	42	dep	LPS	1456:1458	arg1	presence					1444:1451	presence	1444:1451	presence	1444:1451	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS.
36144693	4	43	theme	intense	316:322	arg1	pain					324:327	intense pain	316:327	intense pain	316:327	This disease affects 250 million people worldwide, and is associated with intense pain and loss of articular function.
36144693	15	44	from	studies	1619:1625	arg1	extracts					1642:1649	M. albicans extracts	1630:1649	M. albicans extracts	1630:1649	In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
36144693	0	45	theme	Miconia	60:66	arg1	Sw					78:79	Sw	78:79	Sw	78:79	Phytochemical Evaluation and Anti-Inflammatory Potential of Miconia albicans (Sw.)
36144693	0	45	theme	Miconia	60:66	arg1	albicans					68:75	Miconia albicans	60:75	Miconia albicans (Sw.)	60:81	Phytochemical Evaluation and Anti-Inflammatory Potential of Miconia albicans (Sw.)
36144693	9	46	theme	epithelial	882:891	arg1	Vero					900:903	Vero	900:903	Vero	900:903	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	9	46	theme	epithelial	882:891	arg1	cells					893:897	epithelial cells	882:897	epithelial cells (Vero)	882:904	The extracts were characterized by LC-MS, and their biological activities were evaluated on epithelial cells (Vero), tumoral hepatic cells (Hep-G2), and THP-1 macrophages.
36144693	7	47	theme	ethanolic	656:664	arg1	extracts					666:673	Aqueous and ethanolic extracts	644:673	Aqueous and ethanolic extracts	644:673	Aqueous and ethanolic extracts were prepared.
36144693	14	48	theme	anti-inflammatory	1553:1569	arg1	effects					1571:1577	anti-inflammatory effects	1553:1577	anti-inflammatory effects for the discovery of new drugs	1553:1608	In the future, it will be necessary to identify the phytochemicals that are responsible for anti-inflammatory effects for the discovery of new drugs.
36144693	12	49	theme	antioxidant	1209:1219	arg1	activity					1221:1228	significant antioxidant activity	1197:1228	significant antioxidant activity	1197:1228	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	15	50	theme	action	1710:1715	arg1	mechanisms					1696:1705	their possible mechanisms	1681:1705	their possible mechanisms of action	1681:1715	In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
36144693	2	51	dep	albicans	118:125	arg1	Sw					128:129	Sw	128:129	Sw.	128:130	The plant Miconia albicans (Sw.)
36144693	15	52	theme	M.	1630:1631	arg1	extracts					1642:1649	M. albicans extracts	1630:1649	M. albicans extracts	1630:1649	In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
36144693	14	53	from	future	1468:1473	arg1	necessary					1487:1495	necessary	1487:1495	necessary	1487:1495	In the future, it will be necessary to identify the phytochemicals that are responsible for anti-inflammatory effects for the discovery of new drugs.
36144693	8	54	theme	ethyl	760:764	arg1	acetate					766:772	ethyl acetate	760:772	ethyl acetate	760:772	Afterwards, a liquid-liquid partition was developed using chloroform, ethyl acetate, and n-butanol.
36144693	7	55	theme	Aqueous	644:650	arg1	extracts					666:673	Aqueous and ethanolic extracts	644:673	Aqueous and ethanolic extracts	644:673	Aqueous and ethanolic extracts were prepared.
36144693	10	56	theme	LC-MS	962:966	arg1	analyses					968:975	LC-MS analyses	962:975	LC-MS analyses	962:975	LC-MS analyses identified several flavonoids in all fractions, such as quercetin, myricetin, and their glycosides.
36144693	15	57	dep	In	1611:1612	arg1	vivo					1614:1617	vivo	1614:1617	vivo	1614:1617	In vivo studies on M. albicans extracts are still required to confirm their possible mechanisms of action.
36144693	12	58	theme	ROS	1307:1309	arg1	inhibition					1311:1320	ROS inhibition	1307:1320	ROS inhibition	1307:1320	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36144693	6	59	dep	in	627:628	arg1	vitro					630:634	vitro	630:634	vitro	630:634	Therefore, this study determined the chemical composition of some extracts and evaluated their cytotoxicity, along with their antioxidant and anti-inflammatory, activities using in vitro models.
36144693	6	60	theme	in	627:628	arg1	models					636:641	in vitro models	627:641	in vitro models	627:641	Therefore, this study determined the chemical composition of some extracts and evaluated their cytotoxicity, along with their antioxidant and anti-inflammatory, activities using in vitro models.
36144693	13	61	theme	THP-1	1323:1327	arg1	macrophages					1329:1339	THP-1 macrophages	1323:1339	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL)	1323:1387	THP-1 macrophages treated with the n-butanol fraction (250 µg/mL) released fewer pro-inflammatory cytokines, even in the presence of LPS.
36144693	11	62	theme	n-butanol	1100:1108	arg1	fractions					1110:1118	n-butanol fractions	1100:1118	n-butanol fractions	1100:1118	The crude extracts and n-butanol fractions did not present cytotoxicity to the cells.
36144693	12	63	theme	non-toxic	1167:1175	arg1	fractions					1177:1185	The non-toxic fractions	1163:1185	The non-toxic fractions	1163:1185	The non-toxic fractions presented significant antioxidant activity when evaluated in terms of DPPH scavenging activity, lipid peroxidation, and ROS inhibition.
36463830	7	0	theme	trehalose	1227:1235	arg1	monocorynomycolate					1207:1224	glucose monocorynomycolate	1199:1224	glucose monocorynomycolate	1199:1224	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	0	theme	trehalose	1227:1235	arg1	dicorynomycolate					1237:1252	trehalose dicorynomycolate	1227:1252	trehalose dicorynomycolate	1227:1252	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	0	theme	trehalose	1227:1235	arg1	phospholipids					1259:1271	phospholipids	1259:1271	phospholipids	1259:1271	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	0	theme	trehalose	1227:1235	arg1	lipid					1287:1291	the major lipid	1277:1291	the major lipid in ATCC13869	1277:1304	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	3	1	dep	C.	385:386	arg1	glutamicum					388:397	glutamicum	388:397	glutamicum	388:397	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	7	2	from	lipid	1178:1182	arg1	ΔSYA					1187:1190	ΔSYA	1187:1190	ΔSYA	1187:1190	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	6	3	theme	different	1021:1029	arg1	concentrations					1031:1044	different concentrations	1021:1044	different concentrations of glucose	1021:1055	In minimal medium with different concentrations of glucose, ΔSYA grew worse than ATCC13869 but excreted more L-glutamate.
36463830	8	4	theme	mscCG	1337:1341	arg1	levels					1327:1332	The transcriptional levels	1307:1332	The transcriptional levels of mscCG in ΔSYA	1307:1349	The transcriptional levels of mscCG in ΔSYA was significantly up-regulated when grown in minimal medium.
36463830	0	5	from	role	4:7	arg1	production					75:84	L-glutamate production	63:84	L-glutamate production	63:84	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	0	5	from	role	4:7	arg1	composition					47:57	mycolate composition	38:57	mycolate composition	38:57	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	1	6	theme	industrial	177:186	arg1	production					188:197	the industrial production	173:197	the industrial production of various amino acids	173:220	Corynebacterium glutamicum has been widely utilized for the industrial production of various amino acids.
36463830	5	7	theme	amino	957:961	arg1	acids					963:967	sulfur-containing amino acids	939:967	sulfur-containing amino acids	939:967	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids were down-regulated in ΔSYA.
36463830	8	8	from	levels	1327:1332	arg1	ΔSYA					1346:1349	ΔSYA	1346:1349	ΔSYA	1346:1349	The transcriptional levels of mscCG in ΔSYA was significantly up-regulated when grown in minimal medium.
36463830	3	9	theme	trehalose	531:539	arg1	biosynthesis					541:552	trehalose biosynthesis	531:552	trehalose biosynthesis	531:552	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	0	10	theme	Corynebacterium	89:103	arg1	glutamicum					105:114	Corynebacterium glutamicum	89:114	Corynebacterium glutamicum	89:114	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	0	11	from	composition	47:57	arg1	glutamicum					105:114	Corynebacterium glutamicum	89:114	Corynebacterium glutamicum	89:114	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	2	12	theme	important	308:316	arg1	constituents					318:329	structurally important constituents	295:329	structurally important constituents of the cell envelope	295:350	Trehalose is a prerequisite for the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum.
36463830	2	12	theme	important	308:316	arg1	mycolates					275:283	mycolates	275:283	mycolates which are structurally important constituents of the cell envelope in C. glutamicum	275:367	Trehalose is a prerequisite for the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum.
36463830	3	13	theme	biosynthesis	541:552	arg1	pathways					519:526	the three pathways	509:526	the three pathways of trehalose biosynthesis	509:552	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	6	14	theme	minimal	1001:1007	arg1	medium					1009:1014	minimal medium	1001:1014	minimal medium with different concentrations of glucose	1001:1055	In minimal medium with different concentrations of glucose, ΔSYA grew worse than ATCC13869 but excreted more L-glutamate.
36463830	4	15	theme	monocorynomycolate	679:696	arg1	levels					661:666	similar levels	653:666	similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids	653:743	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	8	16	theme	transcriptional	1311:1325	arg1	levels					1327:1332	The transcriptional levels	1307:1332	The transcriptional levels of mscCG in ΔSYA	1307:1349	The transcriptional levels of mscCG in ΔSYA was significantly up-regulated when grown in minimal medium.
36463830	5	17	theme	genes	846:850	arg1	levels					832:837	Transcriptional levels	816:837	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids	816:967	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids were down-regulated in ΔSYA.
36463830	4	18	theme	control	608:614	arg1	ATCC13869					630:638	the control C. glutamicum ATCC13869	604:638	the control C. glutamicum ATCC13869	604:638	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	5	19	theme	L-arginine	864:873	arg1	biosynthesis					875:886	L-arginine biosynthesis	864:886	L-arginine biosynthesis	864:886	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids were down-regulated in ΔSYA.
36463830	7	20	theme	glucose	1199:1205	arg1	monocorynomycolate					1207:1224	glucose monocorynomycolate	1199:1224	glucose monocorynomycolate	1199:1224	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	20	theme	glucose	1199:1205	arg1	dicorynomycolate					1237:1252	trehalose dicorynomycolate	1227:1252	trehalose dicorynomycolate	1227:1252	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	20	theme	glucose	1199:1205	arg1	phospholipids					1259:1271	phospholipids	1259:1271	phospholipids	1259:1271	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	20	theme	glucose	1199:1205	arg1	lipid					1287:1291	the major lipid	1277:1291	the major lipid in ATCC13869	1277:1304	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	21	from	lipid	1287:1291	arg1	ATCC13869					1296:1304	ATCC13869	1296:1304	ATCC13869	1296:1304	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	1	22	used	utilized	160:167	arg2	glutamicum					133:142	Corynebacterium glutamicum	117:142	Corynebacterium glutamicum	117:142	Corynebacterium glutamicum has been widely utilized for the industrial production of various amino acids.
36463830	3	23	theme	mutant	399:404	arg1	ΔSYA					406:409	C. glutamicum mutant ΔSYA	385:409	C. glutamicum mutant ΔSYA	385:409	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	2	24	theme	envelope	343:350	arg1	constituents					318:329	structurally important constituents	295:329	structurally important constituents of the cell envelope	295:350	Trehalose is a prerequisite for the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum.
36463830	2	24	theme	envelope	343:350	arg1	mycolates					275:283	mycolates	275:283	mycolates which are structurally important constituents of the cell envelope in C. glutamicum	275:367	Trehalose is a prerequisite for the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum.
36463830	1	25	theme	various	202:208	arg1	acids					216:220	various amino acids	202:220	various amino acids	202:220	Corynebacterium glutamicum has been widely utilized for the industrial production of various amino acids.
36463830	5	26	theme	Transcriptional	816:830	arg1	levels					832:837	Transcriptional levels	816:837	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids	816:967	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids were down-regulated in ΔSYA.
36463830	2	27	theme	cell	338:341	arg1	envelope					343:350	the cell envelope	334:350	the cell envelope	334:350	Trehalose is a prerequisite for the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum.
36463830	1	28	theme	amino	210:214	arg1	acids					216:220	various amino acids	202:220	various amino acids	202:220	Corynebacterium glutamicum has been widely utilized for the industrial production of various amino acids.
36463830	0	29	theme	trehalose	12:20	arg1	biosynthesis					22:33	trehalose biosynthesis	12:33	trehalose biosynthesis	12:33	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	1	30	theme	acids	216:220	arg1	production					188:197	the industrial production	173:197	the industrial production of various amino acids	173:220	Corynebacterium glutamicum has been widely utilized for the industrial production of various amino acids.
36463830	4	31	theme	phospholipids	731:743	arg1	levels					661:666	similar levels	653:666	similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids	653:743	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	4	32	theme	fermentation	562:573	arg1	medium					575:580	the fermentation medium	558:580	the fermentation medium	558:580	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	5	33	theme	acids	963:967	arg1	biosynthesis					923:934	the biosynthesis	919:934	the biosynthesis of sulfur-containing amino acids	919:967	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids were down-regulated in ΔSYA.
36463830	1	34	theme	Corynebacterium	117:131	arg1	glutamicum					133:142	Corynebacterium glutamicum	117:142	Corynebacterium glutamicum	117:142	Corynebacterium glutamicum has been widely utilized for the industrial production of various amino acids.
36463830	3	35	theme	genes	480:484	arg1	treY					492:495	treY	492:495	treY	492:495	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	3	35	theme	genes	480:484	arg1	treS					486:489	genes treS	480:489	genes treS	480:489	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	3	35	theme	genes	480:484	arg1	otsA					501:504	otsA	501:504	otsA	501:504	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	0	36	from	production	75:84	arg1	glutamicum					105:114	Corynebacterium glutamicum	89:114	Corynebacterium glutamicum	89:114	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	4	37	theme	similar	653:659	arg1	levels					661:666	similar levels	653:666	similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids	653:743	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	5	38	theme	relevant	852:859	arg1	genes					846:850	the genes	842:850	the genes	842:850	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids were down-regulated in ΔSYA.
36463830	0	39	theme	biosynthesis	22:33	arg1	role					4:7	The role	0:7	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.	0:115	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	7	40	theme	major	1172:1176	arg1	phospholipids					1150:1162	phospholipids	1150:1162	phospholipids	1150:1162	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	40	theme	major	1172:1176	arg1	lipid					1178:1182	the major lipid	1168:1182	the major lipid in ΔSYA	1168:1190	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	3	41	theme	C.	385:386	arg1	ΔSYA					406:409	C. glutamicum mutant ΔSYA	385:409	C. glutamicum mutant ΔSYA	385:409	In this study, C. glutamicum mutant ΔSYA, which is unable to synthesize trehalose was constructed by deleting genes treS, treY and otsA in the three pathways of trehalose biosynthesis.
36463830	8	42	theme	minimal	1396:1402	arg1	medium					1404:1409	minimal medium	1396:1409	minimal medium	1396:1409	The transcriptional levels of mscCG in ΔSYA was significantly up-regulated when grown in minimal medium.
36463830	4	43	dep	C.	616:617	arg1	glutamicum					619:628	glutamicum	619:628	glutamicum	619:628	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	4	44	theme	dicorynomycolate	709:724	arg1	levels					661:666	similar levels	653:666	similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids	653:743	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	0	45	theme	mycolate	38:45	arg1	composition					47:57	mycolate composition	38:57	mycolate composition	38:57	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	4	46	theme	trehalose	699:707	arg1	dicorynomycolate					709:724	trehalose dicorynomycolate	699:724	trehalose dicorynomycolate	699:724	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	6	47	with	medium	1009:1014	arg1	concentrations					1031:1044	different concentrations	1021:1044	different concentrations of glucose	1021:1055	In minimal medium with different concentrations of glucose, ΔSYA grew worse than ATCC13869 but excreted more L-glutamate.
36463830	4	48	theme	L-glutamate	788:798	arg1	times					777:781	12.5 times	772:781	12.5 times more L-glutamate	772:798	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	2	49	theme	mycolates	275:283	arg1	biosynthesis					259:270	the biosynthesis	255:270	the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum	255:367	Trehalose is a prerequisite for the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum.
36463830	5	50	theme	sulfur-containing	939:955	arg1	acids					963:967	sulfur-containing amino acids	939:967	sulfur-containing amino acids	939:967	Transcriptional levels of the genes relevant to L-arginine biosynthesis, encoding ODHC and relevant to the biosynthesis of sulfur-containing amino acids were down-regulated in ΔSYA.
36463830	2	51	from	constituents	318:329	arg1	glutamicum					358:367	C. glutamicum	355:367	C. glutamicum	355:367	Trehalose is a prerequisite for the biosynthesis of mycolates which are structurally important constituents of the cell envelope in C. glutamicum.
36463830	6	52	theme	glucose	1049:1055	arg1	concentrations					1031:1044	different concentrations	1021:1044	different concentrations of glucose	1021:1055	In minimal medium with different concentrations of glucose, ΔSYA grew worse than ATCC13869 but excreted more L-glutamate.
36463830	0	53	theme	L-glutamate	63:73	arg1	production					75:84	L-glutamate production	63:84	L-glutamate production	63:84	The role of trehalose biosynthesis on mycolate composition and L-glutamate production in Corynebacterium glutamicum.
36463830	4	54	theme	C.	616:617	arg1	ATCC13869					630:638	the control C. glutamicum ATCC13869	604:638	the control C. glutamicum ATCC13869	604:638	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	4	55	theme	glucose	671:677	arg1	monocorynomycolate					679:696	glucose monocorynomycolate	671:696	glucose monocorynomycolate	671:696	In the fermentation medium, ΔSYA grew as well as the control C. glutamicum ATCC13869, synthesized similar levels of glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids to ATCC13869, but produced 12.5 times more L-glutamate than ATCC13869.
36463830	7	56	theme	major	1281:1285	arg1	monocorynomycolate					1207:1224	glucose monocorynomycolate	1199:1224	glucose monocorynomycolate	1199:1224	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	56	theme	major	1281:1285	arg1	dicorynomycolate					1237:1252	trehalose dicorynomycolate	1227:1252	trehalose dicorynomycolate	1227:1252	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	56	theme	major	1281:1285	arg1	phospholipids					1259:1271	phospholipids	1259:1271	phospholipids	1259:1271	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	56	theme	major	1281:1285	arg1	lipid					1287:1291	the major lipid	1277:1291	the major lipid in ATCC13869	1277:1304	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
36463830	7	57	theme	minimal	1134:1140	arg1	medium					1142:1147	minimal medium	1134:1147	minimal medium	1134:1147	When grown in minimal medium, phospholipids are the major lipid in ΔSYA, while glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids are the major lipid in ATCC13869.
37001665	1	0	theme	ilex	250:253	arg1	L.					255:256	Quercus ilex L.	242:256	Quercus ilex L.	242:256	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	8	1	theme	incomplete	1355:1364	arg1	recovery					1366:1373	an incomplete recovery	1352:1373	an incomplete recovery of leaf gas exchanges	1352:1395	After rewatering, the presence of residual xylem embolism led to an incomplete recovery of leaf gas exchanges.
37001665	1	2	from	events	300:305	arg1	forests					329:335	many Mediterranean forests	310:335	many Mediterranean forests	310:335	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	4	3	from	relations	689:697	arg1	parenchyma					794:803	wood parenchyma	789:803	wood parenchyma	789:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	9	4	theme	partial	1411:1417	arg1	restoration					1419:1429	the partial restoration	1407:1429	the partial restoration of photosynthesis	1407:1447	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	9	5	theme	starch	1481:1486	arg1	reserves					1488:1495	new starch reserves	1477:1495	new starch reserves in the wood parenchyma	1477:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	7	6	from	metabolism	1252:1261	arg1	parenchyma					1275:1284	the wood parenchyma	1266:1284	the wood parenchyma	1266:1284	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	10	7	theme	wood	1621:1624	arg1	fibers					1637:1642	the wood parenchyma fibers	1617:1642	the wood parenchyma fibers	1617:1642	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	4	8	theme	relations	689:697	arg1	Measurements					648:659	Measurements	648:659	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma	648:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	11	9	theme	Q.	1777:1778	arg1	seedlings					1785:1793	Q. ilex seedlings	1777:1793	Q. ilex seedlings	1777:1793	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	7	10	theme	gene	1104:1107	arg1	BAM3					1109:1112	the β-amylase gene BAM3	1090:1112	the β-amylase gene BAM3	1090:1112	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	1	11	theme	few	185:187	arg1	decades					189:195	the last few decades	176:195	the last few decades	176:195	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	9	12	from	reserves	1488:1495	arg1	parenchyma					1509:1518	the wood parenchyma	1500:1518	the wood parenchyma	1500:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	3	13	theme	Q.	583:584	arg1	seedlings					591:599	Q. ilex seedlings	583:599	Q. ilex seedlings exposed to severe water stress and rewatering	583:645	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	6	14	theme	stomatal	974:981	arg1	conductance					983:993	stomatal conductance	974:993	stomatal conductance	974:993	A 70 % reduction in stomatal conductance led to a strong depletion of wood NSCs.
37001665	4	15	theme	gas	669:671	arg1	exchange					673:680	leaf gas exchange	664:680	leaf gas exchange	664:680	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	0	16	theme	hydraulic	129:137	arg1	functionality					139:151	xylem hydraulic functionality	123:151	xylem hydraulic functionality	123:151	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	0	16	theme	hydraulic	129:137	arg1	Changes					82:88	Changes	82:88	Changes in non-structural carbohydrates	82:120	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	3	17	theme	water	619:623	arg1	stress					625:630	severe water stress	612:630	severe water stress	612:630	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	1	18	theme	dieback	208:214	arg1	episodes					230:237	extensive dieback and mortality episodes	198:237	extensive dieback and mortality episodes of Quercus ilex L.	198:256	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	4	19	theme	gene	746:749	arg1	expression					751:760	drought-related gene expression	730:760	drought-related gene expression	730:760	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	10	20	theme	cell	1592:1595	arg1	composition					1602:1612	the cell wall composition	1588:1612	the cell wall composition of the wood parenchyma fibers	1588:1642	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	11	21	theme	seedlings	1785:1793	arg1	reserves					1748:1755	NSCs reserves	1743:1755	NSCs reserves	1743:1755	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	11	21	theme	seedlings	1785:1793	arg1	rates					1768:1772	growth rates	1761:1772	growth rates	1761:1772	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	5	22	theme	conductivity	940:951	arg1	loss					922:925	a ~ 50 % loss	913:925	a ~ 50 % loss of hydraulic conductivity	913:951	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	6	23	theme	70 	956:958	arg1	%					959:959	%	959:959	%	959:959	A 70 % reduction in stomatal conductance led to a strong depletion of wood NSCs.
37001665	0	24	from	Changes	82:88	arg1	carbohydrates					108:120	non-structural carbohydrates	93:120	non-structural carbohydrates	93:120	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	4	25	theme	non-structural	700:713	arg1	carbohydrates					715:727	non-structural carbohydrates	700:727	non-structural carbohydrates	700:727	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	0	26	theme	severe	61:66	arg1	stress					74:79	severe water stress	61:79	severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy	61:168	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	1	27	theme	Mediterranean	315:327	arg1	forests					329:335	many Mediterranean forests	310:335	many Mediterranean forests	310:335	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	5	28	theme	%	920:920	arg1	loss					922:925	a ~ 50 % loss	913:925	a ~ 50 % loss of hydraulic conductivity	913:951	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	6	29	theme	wood	1024:1027	arg1	NSCs					1029:1032	wood NSCs	1024:1032	wood NSCs	1024:1032	A 70 % reduction in stomatal conductance led to a strong depletion of wood NSCs.
37001665	8	30	theme	residual	1321:1328	arg1	embolism					1336:1343	residual xylem embolism	1321:1343	residual xylem embolism	1321:1343	After rewatering, the presence of residual xylem embolism led to an incomplete recovery of leaf gas exchanges.
37001665	10	31	located	observed	1649:1656	arg2	changes					1577:1583	changes	1577:1583	changes in the cell wall composition of the wood parenchyma fibers	1577:1642	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	10	31	located	observed	1649:1656	arg1	addition					1567:1574	addition	1567:1574	addition	1567:1574	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	9	32	from	production	1528:1537	arg1	parenchyma					1509:1518	the wood parenchyma	1500:1518	the wood parenchyma	1500:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	8	33	theme	embolism	1336:1343	arg1	presence					1309:1316	the presence	1305:1316	the presence of residual xylem embolism	1305:1343	After rewatering, the presence of residual xylem embolism led to an incomplete recovery of leaf gas exchanges.
37001665	11	34	theme	NSCs	1743:1746	arg1	reserves					1748:1755	NSCs reserves	1743:1755	NSCs reserves	1743:1755	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	4	35	from	parenchyma	794:803	arg1	Measurements					648:659	Measurements	648:659	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma	648:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	10	36	theme	fibers	1637:1642	arg1	composition					1602:1612	the cell wall composition	1588:1612	the cell wall composition of the wood parenchyma fibers	1588:1642	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	0	37	theme	non-structural	93:106	arg1	carbohydrates					108:120	non-structural carbohydrates	93:120	non-structural carbohydrates	93:120	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	9	38	theme	narrower	1546:1553	arg1	vessels					1555:1561	new narrower vessels	1542:1561	new narrower vessels	1542:1561	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	0	39	theme	water	68:72	arg1	stress					74:79	severe water stress	61:79	severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy	61:168	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	4	40	theme	anatomical	767:776	arg1	changes					778:784	anatomical changes	767:784	anatomical changes in wood parenchyma	767:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	2	41	theme	anatomical	377:386	arg1	mechanisms					405:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	However, the underlying physiological, anatomical, and biochemical mechanisms remain poorly understood.
37001665	9	42	theme	vessels	1555:1561	arg1	accumulation					1461:1472	the accumulation	1457:1472	the accumulation of new starch reserves in the wood parenchyma	1457:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	9	42	theme	vessels	1555:1561	arg1	production					1528:1537	the production	1524:1537	the production of new narrower vessels	1524:1561	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	0	43	dep	stress	74:79	arg1	functionality					139:151	xylem hydraulic functionality	123:151	xylem hydraulic functionality	123:151	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	0	43	dep	stress	74:79	arg1	anatomy					162:168	wood anatomy	157:168	wood anatomy	157:168	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	0	43	dep	stress	74:79	arg1	Changes					82:88	Changes	82:88	Changes in non-structural carbohydrates	82:120	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	9	44	from	parenchyma	1509:1518	arg1	accumulation					1461:1472	the accumulation	1457:1472	the accumulation of new starch reserves in the wood parenchyma	1457:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	9	44	from	parenchyma	1509:1518	arg1	production					1528:1537	the production	1524:1537	the production of new narrower vessels	1524:1561	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	5	45	theme	stem	851:854	arg1	potential					862:870	the midday stem water potential	840:870	the midday stem water potential	840:870	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	9	46	theme	wood	1504:1507	arg1	parenchyma					1509:1518	the wood parenchyma	1500:1518	the wood parenchyma	1500:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	8	47	theme	gas	1383:1385	arg1	exchanges					1387:1395	leaf gas exchanges	1378:1395	leaf gas exchanges	1378:1395	After rewatering, the presence of residual xylem embolism led to an incomplete recovery of leaf gas exchanges.
37001665	0	48	theme	Quercus	35:41	arg1	seedlings					48:56	Quercus ilex seedlings	35:56	Quercus ilex seedlings	35:56	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	1	49	theme	drought	292:298	arg1	events					300:305	severe drought events	285:305	severe drought events in many Mediterranean forests	285:335	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	0	50	theme	seedlings	48:56	arg1	mechanisms					21:30	resilience mechanisms	10:30	resilience mechanisms of Quercus ilex seedlings	10:56	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	11	51	theme	carryover	1808:1816	arg1	effects					1818:1824	the carryover effects	1804:1824	the carryover effects of water stress	1804:1840	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	4	52	from	expression	751:760	arg1	parenchyma					794:803	wood parenchyma	789:803	wood parenchyma	789:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	2	53	theme	biochemical	393:403	arg1	mechanisms					405:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	However, the underlying physiological, anatomical, and biochemical mechanisms remain poorly understood.
37001665	3	54	theme	non-structural	535:548	arg1	NSCs					565:568	NSCs	565:568	NSCs	565:568	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	3	54	theme	non-structural	535:548	arg1	carbohydrates					550:562	non-structural carbohydrates	535:562	non-structural carbohydrates (NSCs)	535:569	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	10	55	from	changes	1577:1583	arg1	composition					1602:1612	the cell wall composition	1588:1612	the cell wall composition of the wood parenchyma fibers	1588:1642	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	3	56	attach	linked	502:507	arg2	processes					492:500	the physiological and biochemical processes	458:500	the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics	458:578	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	3	56	attach	linked	502:507	arg1	NSCs					565:568	NSCs	565:568	NSCs	565:568	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	3	56	attach	linked	502:507	arg1	formation					521:529	embolism formation	512:529	embolism formation	512:529	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	3	56	attach	linked	502:507	arg1	carbohydrates					550:562	non-structural carbohydrates	535:562	non-structural carbohydrates (NSCs)	535:569	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	11	57	theme	stress	1835:1840	arg1	effects					1818:1824	the carryover effects	1804:1824	the carryover effects of water stress	1804:1840	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	4	58	from	carbohydrates	715:727	arg1	parenchyma					794:803	wood parenchyma	789:803	wood parenchyma	789:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	1	59	theme	Quercus	242:248	arg1	L.					255:256	Quercus ilex L.	242:256	Quercus ilex L.	242:256	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	1	60	theme	L.	255:256	arg1	episodes					230:237	extensive dieback and mortality episodes	198:237	extensive dieback and mortality episodes of Quercus ilex L.	198:256	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	7	61	theme	BAM3	1109:1112	arg1	upregulation					1074:1085	the upregulation	1070:1085	the upregulation of the β-amylase gene BAM3	1070:1112	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	9	62	theme	photosynthesis	1434:1447	arg1	restoration					1419:1429	the partial restoration	1407:1429	the partial restoration of photosynthesis	1407:1447	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	7	63	theme	glucose	1151:1157	arg1	downregulation					1133:1146	the downregulation	1129:1146	the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes	1129:1200	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	4	64	theme	exchange	673:680	arg1	Measurements					648:659	Measurements	648:659	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma	648:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	7	65	dep	together	1115:1122	arg1	with					1124:1127	with	1124:1127	with	1124:1127	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	7	66	theme	β-amylase	1094:1102	arg1	BAM3					1109:1112	the β-amylase gene BAM3	1090:1112	the β-amylase gene BAM3	1090:1112	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	3	67	theme	ilex	586:589	arg1	seedlings					591:599	Q. ilex seedlings	583:599	Q. ilex seedlings exposed to severe water stress and rewatering	583:645	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	4	68	from	exchange	673:680	arg1	parenchyma					794:803	wood parenchyma	789:803	wood parenchyma	789:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	1	69	theme	last	180:183	arg1	decades					189:195	the last few decades	176:195	the last few decades	176:195	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	4	70	theme	water	683:687	arg1	relations					689:697	water relations	683:697	water relations	683:697	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	11	71	theme	ilex	1780:1783	arg1	seedlings					1785:1793	Q. ilex seedlings	1777:1793	Q. ilex seedlings	1777:1793	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	0	72	theme	xylem	123:127	arg1	functionality					139:151	xylem hydraulic functionality	123:151	xylem hydraulic functionality	123:151	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	0	72	theme	xylem	123:127	arg1	Changes					82:88	Changes	82:88	Changes in non-structural carbohydrates	82:120	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	5	73	theme	water	856:860	arg1	potential					862:870	the midday stem water potential	840:870	the midday stem water potential	840:870	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	2	74	theme	physiological	362:374	arg1	mechanisms					405:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	However, the underlying physiological, anatomical, and biochemical mechanisms remain poorly understood.
37001665	11	75	theme	water	1829:1833	arg1	stress					1835:1840	water stress	1829:1840	water stress	1829:1840	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	3	76	theme	severe	612:617	arg1	stress					625:630	severe water stress	612:630	severe water stress	612:630	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	4	77	from	Measurements	648:659	arg1	parenchyma					794:803	wood parenchyma	789:803	wood parenchyma	789:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	1	78	theme	extensive	198:206	arg1	dieback					208:214	extensive dieback	198:214	extensive dieback	198:214	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	4	79	theme	leaf	664:667	arg1	exchange					673:680	leaf gas exchange	664:680	leaf gas exchange	664:680	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	10	80	theme	wall	1597:1600	arg1	composition					1602:1612	the cell wall composition	1588:1612	the cell wall composition of the wood parenchyma fibers	1588:1642	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	0	81	theme	wood	157:160	arg1	anatomy					162:168	wood anatomy	157:168	wood anatomy	157:168	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	0	81	theme	wood	157:160	arg1	Changes					82:88	Changes	82:88	Changes in non-structural carbohydrates	82:120	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	7	82	theme	Starch	1035:1040	arg1	consumption					1042:1052	Starch consumption	1035:1052	Starch consumption	1035:1052	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	5	83	theme	hydraulic	930:938	arg1	conductivity					940:951	hydraulic conductivity	930:951	hydraulic conductivity	930:951	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	6	84	theme	%	959:959	arg1	reduction					961:969	A 70 % reduction	954:969	A 70 % reduction in stomatal conductance	954:993	A 70 % reduction in stomatal conductance led to a strong depletion of wood NSCs.
37001665	4	85	theme	drought-related	730:744	arg1	expression					751:760	drought-related gene expression	730:760	drought-related gene expression	730:760	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	11	86	dep	reserves	1748:1755	arg1	the					1739:1741	the	1739:1741	the	1739:1741	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	4	87	theme	expression	751:760	arg1	Measurements					648:659	Measurements	648:659	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma	648:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	7	88	theme	glucose	1212:1218	arg1	utilization					1220:1230	glucose utilization	1212:1230	glucose utilization to sustain cellular metabolism in the wood parenchyma	1212:1284	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	5	89	theme	a ~ 50 	913:919	arg1	%					920:920	%	920:920	%	920:920	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	6	90	theme	NSCs	1029:1032	arg1	depletion					1011:1019	a strong depletion	1002:1019	a strong depletion of wood NSCs	1002:1032	A 70 % reduction in stomatal conductance led to a strong depletion of wood NSCs.
37001665	4	91	theme	carbohydrates	715:727	arg1	Measurements					648:659	Measurements	648:659	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma	648:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	11	92	theme	growth	1761:1766	arg1	rates					1768:1772	growth rates	1761:1772	growth rates	1761:1772	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	7	93	theme	transport	1186:1194	arg1	genes					1196:1200	transport genes	1186:1200	transport genes	1186:1200	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	6	94	theme	strong	1004:1009	arg1	depletion					1011:1019	a strong depletion	1002:1019	a strong depletion of wood NSCs	1002:1032	A 70 % reduction in stomatal conductance led to a strong depletion of wood NSCs.
37001665	8	95	theme	xylem	1330:1334	arg1	embolism					1336:1343	residual xylem embolism	1321:1343	residual xylem embolism	1321:1343	After rewatering, the presence of residual xylem embolism led to an incomplete recovery of leaf gas exchanges.
37001665	4	96	theme	wood	789:792	arg1	parenchyma					794:803	wood parenchyma	789:803	wood parenchyma	789:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	9	97	theme	new	1542:1544	arg1	vessels					1555:1561	new narrower vessels	1542:1561	new narrower vessels	1542:1561	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	7	98	dep	glucose	1151:1157	arg1	genes					1196:1200	transport genes	1186:1200	transport genes	1186:1200	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	9	99	theme	reserves	1488:1495	arg1	accumulation					1461:1472	the accumulation	1457:1472	the accumulation of new starch reserves in the wood parenchyma	1457:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	9	99	theme	reserves	1488:1495	arg1	production					1528:1537	the production	1524:1537	the production of new narrower vessels	1524:1561	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	10	100	theme	parenchyma	1626:1635	arg1	fibers					1637:1642	the wood parenchyma fibers	1617:1642	the wood parenchyma fibers	1617:1642	In addition, changes in the cell wall composition of the wood parenchyma fibers were observed.
37001665	0	101	theme	resilience	10:19	arg1	mechanisms					21:30	resilience mechanisms	10:30	resilience mechanisms of Quercus ilex seedlings	10:56	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	4	102	from	changes	778:784	arg1	parenchyma					794:803	wood parenchyma	789:803	wood parenchyma	789:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	3	103	dep	formation	521:529	arg1	dynamics					571:578	dynamics	571:578	dynamics	571:578	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	11	104	theme	hydraulic	1867:1875	arg1	dysfunction					1877:1887	hydraulic dysfunction	1867:1887	hydraulic dysfunction	1867:1887	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	5	105	theme	midday	844:849	arg1	potential					862:870	the midday stem water potential	840:870	the midday stem water potential	840:870	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	4	106	theme	changes	778:784	arg1	Measurements					648:659	Measurements	648:659	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma	648:803	Measurements of leaf gas exchange, water relations, non-structural carbohydrates, drought-related gene expression, and anatomical changes in wood parenchyma were assessed.
37001665	3	107	theme	biochemical	480:490	arg1	processes					492:500	the physiological and biochemical processes	458:500	the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics	458:578	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	0	108	theme	ilex	43:46	arg1	seedlings					48:56	Quercus ilex seedlings	35:56	Quercus ilex seedlings	35:56	Unveiling resilience mechanisms of Quercus ilex seedlings to severe water stress: Changes in non-structural carbohydrates, xylem hydraulic functionality and wood anatomy.
37001665	1	109	theme	severe	285:290	arg1	events					300:305	severe drought events	285:305	severe drought events in many Mediterranean forests	285:335	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	8	110	theme	leaf	1378:1381	arg1	exchanges					1387:1395	leaf gas exchanges	1378:1395	leaf gas exchanges	1378:1395	After rewatering, the presence of residual xylem embolism led to an incomplete recovery of leaf gas exchanges.
37001665	3	111	theme	physiological	462:474	arg1	processes					492:500	the physiological and biochemical processes	458:500	the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics	458:578	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	5	112	theme	water	826:830	arg1	stress					832:837	water stress	826:837	water stress	826:837	Under water stress, the midday stem water potential dropped below - 4.5 MPa corresponding to a ~ 50 % loss of hydraulic conductivity.
37001665	7	113	theme	wood	1270:1273	arg1	parenchyma					1275:1284	the wood parenchyma	1266:1284	the wood parenchyma	1266:1284	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	11	114	theme	rewatering	1701:1710	arg1	days					1693:1696	thirty days	1686:1696	thirty days of rewatering	1686:1710	Our findings indicate that thirty days of rewatering were sufficient to restore the NSCs reserves and growth rates of Q. ilex seedlings and that the carryover effects of water stress were primarily caused by hydraulic dysfunction.
37001665	8	115	theme	exchanges	1387:1395	arg1	recovery					1366:1373	an incomplete recovery	1352:1373	an incomplete recovery of leaf gas exchanges	1352:1395	After rewatering, the presence of residual xylem embolism led to an incomplete recovery of leaf gas exchanges.
37001665	3	116	theme	embolism	512:519	arg1	formation					521:529	embolism formation	512:529	embolism formation	512:529	We investigated the physiological and biochemical processes linked to embolism formation and non-structural carbohydrates (NSCs) dynamics in Q. ilex seedlings exposed to severe water stress and rewatering.
37001665	1	117	theme	many	310:313	arg1	forests					329:335	many Mediterranean forests	310:335	many Mediterranean forests	310:335	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	9	118	from	accumulation	1461:1472	arg1	parenchyma					1509:1518	the wood parenchyma	1500:1518	the wood parenchyma	1500:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	6	119	from	reduction	961:969	arg1	conductance					983:993	stomatal conductance	974:993	stomatal conductance	974:993	A 70 % reduction in stomatal conductance led to a strong depletion of wood NSCs.
37001665	2	120	theme	underlying	351:360	arg1	mechanisms					405:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	the underlying physiological, anatomical, and biochemical mechanisms	347:414	However, the underlying physiological, anatomical, and biochemical mechanisms remain poorly understood.
37001665	1	121	theme	mortality	220:228	arg1	episodes					230:237	extensive dieback and mortality episodes	198:237	extensive dieback and mortality episodes of Quercus ilex L.	198:256	Over the last few decades, extensive dieback and mortality episodes of Quercus ilex L. have been documented after severe drought events in many Mediterranean forests.
37001665	9	122	theme	new	1477:1479	arg1	reserves					1488:1495	new starch reserves	1477:1495	new starch reserves in the wood parenchyma	1477:1518	However, the partial restoration of photosynthesis allowed the accumulation of new starch reserves in the wood parenchyma and the production of new narrower vessels.
37001665	7	123	theme	cellular	1243:1250	arg1	metabolism					1252:1261	cellular metabolism	1243:1261	cellular metabolism in the wood parenchyma	1243:1284	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
37001665	7	124	theme	sucrose	1170:1176	arg1	downregulation					1133:1146	the downregulation	1129:1146	the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes	1129:1200	Starch consumption, resulting from the upregulation of the β-amylase gene BAM3, together with the downregulation of glucose (GPT1) and sucrose (SUC27) transport genes, suggests glucose utilization to sustain cellular metabolism in the wood parenchyma.
36890624	4	0	theme	model	653:657	arg1	mice					659:662	AD model mice	650:662	AD model mice	650:662	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	4	1	theme	16 S	543:546	arg1	sequencing					558:567	high-throughput 16 S rRNA gene sequencing	527:567	high-throughput 16 S rRNA gene sequencing	527:567	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	9	2	theme	gut	1621:1623	arg1	microbiota					1625:1634	the gut microbiota	1617:1634	the gut microbiota	1617:1634	CONCLUSION The imbalance of the gut microbiota and metabolites plays a role in the progression of AD; β-glucan blocks the development of AD by improving the gut microbiota and its metabolites and reducing neuroinflammation.
36890624	4	3	theme	AD	650:651	arg1	mice					659:662	AD model mice	650:662	AD model mice	650:662	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	4	4	theme	high-throughput	527:541	arg1	sequencing					558:567	high-throughput 16 S rRNA gene sequencing	527:567	high-throughput 16 S rRNA gene sequencing	527:567	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	7	5	theme	flora	1156:1160	arg1	metabolites					1162:1172	the flora metabolites	1152:1172	the flora metabolites in the intestinal content	1152:1198	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	1	6	theme	cognitive	322:330	arg1	function					332:339	cognitive function	322:339	cognitive function	322:339	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	3	7	theme	cognitive	495:503	arg1	function					505:512	cognitive function	495:512	cognitive function	495:512	METHOD This study used behavioral testing to measure cognitive function.
36890624	4	8	theme	gene	553:556	arg1	sequencing					558:567	high-throughput 16 S rRNA gene sequencing	527:567	high-throughput 16 S rRNA gene sequencing	527:567	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	9	9	theme	gut	1496:1498	arg1	imbalance					1479:1487	The imbalance	1475:1487	The imbalance of the gut microbiota and metabolites	1475:1525	CONCLUSION The imbalance of the gut microbiota and metabolites plays a role in the progression of AD; β-glucan blocks the development of AD by improving the gut microbiota and its metabolites and reducing neuroinflammation.
36890624	9	10	dep	gut	1496:1498	arg1	metabolites					1515:1525	metabolites	1515:1525	metabolites	1515:1525	CONCLUSION The imbalance of the gut microbiota and metabolites plays a role in the progression of AD; β-glucan blocks the development of AD by improving the gut microbiota and its metabolites and reducing neuroinflammation.
36890624	9	10	dep	gut	1496:1498	arg1	microbiota					1500:1509	microbiota	1500:1509	microbiota	1500:1509	CONCLUSION The imbalance of the gut microbiota and metabolites plays a role in the progression of AD; β-glucan blocks the development of AD by improving the gut microbiota and its metabolites and reducing neuroinflammation.
36890624	6	11	theme	appropriate	889:899	arg1	supplementation					901:915	appropriate supplementation	889:915	appropriate supplementation of β-glucan during the progression of AD	889:956	RESULTS We found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition.
36890624	4	12	theme	intestinal	710:719	arg1	flora					721:725	intestinal flora	710:725	intestinal flora	710:725	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	4	13	theme	rRNA	548:551	arg1	sequencing					558:567	high-throughput 16 S rRNA gene sequencing	527:567	high-throughput 16 S rRNA gene sequencing	527:567	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	7	14	theme	flora	1128:1132	arg1	composition					1098:1108	the composition	1094:1108	the composition of the intestinal flora	1094:1132	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	7	15	theme	cerebral	1271:1278	arg1	cortex					1280:1285	the cerebral cortex	1267:1285	the cerebral cortex	1267:1285	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	3	16	dep	METHOD	442:447	arg1	used					460:463	used	460:463	used behavioral testing to measure cognitive function	460:512	METHOD This study used behavioral testing to measure cognitive function.
36890624	4	17	theme	microbiota	615:624	arg1	SCFAs					641:645	the intestinal microbiota and metabolite SCFAs	600:645	the intestinal microbiota and metabolite SCFAs of AD model mice	600:662	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	0	18	theme	mice	52:55	arg1	impairment					30:39	cognitive impairment	20:39	cognitive impairment of APP/PS1 mice	20:55	β-Glucan attenuates cognitive impairment of APP/PS1 mice via regulating intestinal flora and its metabolites.
36890624	5	19	from	expressions	763:773	arg1	brain					812:816	the mouse brain	802:816	the mouse brain	802:816	Finally, the expressions of inflammatory factors in the mouse brain were detected by Western blot and Elisa methods.
36890624	6	20	dep	RESULTS	867:873	arg1	found					878:882	found	878:882	found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition	878:1022	RESULTS We found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition.
36890624	4	21	theme	intestinal	604:613	arg1	microbiota					615:624	intestinal microbiota	604:624	intestinal microbiota	604:624	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	10	22	theme	AD	1742:1743	arg1	treatment					1729:1737	the treatment	1725:1737	the treatment of AD	1725:1743	β-Glucan is a potential strategy for the treatment of AD by reshaping the gut microbiota and improving its metabolites.
36890624	1	23	theme	intestinal	353:362	arg1	flora					364:368	the intestinal flora	349:368	the intestinal flora	349:368	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	7	24	theme	microglia	1254:1262	arg1	activation					1215:1224	the activation	1211:1224	the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus	1211:1301	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	7	25	theme	intestinal	1181:1190	arg1	content					1192:1198	the intestinal content	1177:1198	the intestinal content	1177:1198	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	10	26	theme	gut	1762:1764	arg1	microbiota					1766:1775	the gut microbiota	1758:1775	the gut microbiota	1758:1775	β-Glucan is a potential strategy for the treatment of AD by reshaping the gut microbiota and improving its metabolites.
36890624	4	27	theme	metabolite	630:639	arg1	SCFAs					641:645	the intestinal microbiota and metabolite SCFAs	600:645	the intestinal microbiota and metabolite SCFAs of AD model mice	600:662	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	1	28	attach	derived	269:275	arg2	β-glucan					242:249	β-glucan	242:249	β-glucan	242:249	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	1	28	attach	derived	269:275	arg2	polysaccharide					254:267	a polysaccharide	252:267	a polysaccharide derived from Saccharomyces cerevisiae	252:305	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	1	28	attach	derived	269:275	arg1	cerevisiae					296:305	Saccharomyces cerevisiae	282:305	Saccharomyces cerevisiae	282:305	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	5	29	theme	Western	835:841	arg1	blot					843:846	Western blot	835:846	Western blot	835:846	Finally, the expressions of inflammatory factors in the mouse brain were detected by Western blot and Elisa methods.
36890624	5	30	theme	inflammatory	778:789	arg1	factors					791:797	inflammatory factors	778:797	inflammatory factors	778:797	Finally, the expressions of inflammatory factors in the mouse brain were detected by Western blot and Elisa methods.
36890624	7	31	from	activation	1215:1224	arg1	hippocampus					1291:1301	hippocampus	1291:1301	hippocampus	1291:1301	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	7	31	from	activation	1215:1224	arg1	cortex					1280:1285	the cerebral cortex	1267:1285	the cerebral cortex	1267:1285	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	5	32	dep	blot	843:846	arg1	methods					858:864	methods	858:864	methods	858:864	Finally, the expressions of inflammatory factors in the mouse brain were detected by Western blot and Elisa methods.
36890624	8	33	theme	inflammatory	1364:1375	arg1	factors					1377:1383	inflammatory factors	1364:1383	inflammatory factors	1364:1383	While reducing the expression of inflammatory factors in the hippocampus and cerebral cortex, thereby controlling neuroinflammation.
36890624	0	34	theme	cognitive	20:28	arg1	impairment					30:39	cognitive impairment	20:39	cognitive impairment of APP/PS1 mice	20:55	β-Glucan attenuates cognitive impairment of APP/PS1 mice via regulating intestinal flora and its metabolites.
36890624	7	35	from	changes	1083:1089	arg1	composition					1098:1108	the composition	1094:1108	the composition of the intestinal flora	1094:1132	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	6	36	theme	plaque	1006:1011	arg1	deposition					1013:1022	A β plaque deposition	1002:1022	A β plaque deposition	1002:1022	RESULTS We found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition.
36890624	0	37	theme	APP/PS1	44:50	arg1	mice					52:55	APP/PS1 mice	44:55	APP/PS1 mice	44:55	β-Glucan attenuates cognitive impairment of APP/PS1 mice via regulating intestinal flora and its metabolites.
36890624	4	38	theme	mice	659:662	arg1	SCFAs					641:645	the intestinal microbiota and metabolite SCFAs	600:645	the intestinal microbiota and metabolite SCFAs of AD model mice	600:662	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	6	39	theme	β	1004:1004	arg1	deposition					1013:1022	A β plaque deposition	1002:1022	A β plaque deposition	1002:1022	RESULTS We found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition.
36890624	6	40	theme	β-glucan	920:927	arg1	supplementation					901:915	appropriate supplementation	889:915	appropriate supplementation of β-glucan during the progression of AD	889:956	RESULTS We found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition.
36890624	5	41	theme	mouse	806:810	arg1	brain					812:816	the mouse brain	802:816	the mouse brain	802:816	Finally, the expressions of inflammatory factors in the mouse brain were detected by Western blot and Elisa methods.
36890624	7	42	theme	intestinal	1117:1126	arg1	flora					1128:1132	the intestinal flora	1113:1132	the intestinal flora	1113:1132	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	1	43	theme	disease	206:212	arg1	progression					179:189	the progression	175:189	the progression of Alzheimer's disease (AD)	175:217	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	7	44	from	metabolites	1162:1172	arg1	content					1192:1198	the intestinal content	1177:1198	the intestinal content	1177:1198	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	7	45	theme	factors	1242:1248	arg1	activation					1215:1224	the activation	1211:1224	the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus	1211:1301	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	2	46	theme	β-glucan	414:421	arg1	effect					404:409	this effect	399:409	this effect of β-glucan	399:421	However, it is not known if this effect of β-glucan is involved in AD.
36890624	1	47	theme	Saccharomyces	282:294	arg1	cerevisiae					296:305	Saccharomyces cerevisiae	282:305	Saccharomyces cerevisiae	282:305	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	8	48	theme	factors	1377:1383	arg1	expression					1350:1359	the expression	1346:1359	the expression of inflammatory factors in the hippocampus and cerebral cortex	1346:1422	While reducing the expression of inflammatory factors in the hippocampus and cerebral cortex, thereby controlling neuroinflammation.
36890624	1	49	theme	intestinal	125:134	arg1	flora					136:140	The intestinal flora	121:140	The intestinal flora	121:140	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	7	50	theme	brain-gut	1315:1323	arg1	axis					1325:1328	the brain-gut axis	1311:1328	the brain-gut axis	1311:1328	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	9	51	dep	CONCLUSION	1464:1473	arg1	plays					1527:1531	plays	1527:1531	plays a role in the progression of AD	1527:1563	CONCLUSION The imbalance of the gut microbiota and metabolites plays a role in the progression of AD; β-glucan blocks the development of AD by improving the gut microbiota and its metabolites and reducing neuroinflammation.
36890624	3	52	used	used	460:463	arg2	study					454:458	This study	449:458	This study	449:458	METHOD This study used behavioral testing to measure cognitive function.
36890624	7	53	theme	β-glucan	1057:1064	arg1	supplementation					1038:1052	supplementation	1038:1052	supplementation of β-glucan	1038:1064	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	9	54	theme	AD	1562:1563	arg1	progression					1547:1557	the progression	1543:1557	the progression of AD	1543:1563	CONCLUSION The imbalance of the gut microbiota and metabolites plays a role in the progression of AD; β-glucan blocks the development of AD by improving the gut microbiota and its metabolites and reducing neuroinflammation.
36890624	10	55	theme	potential	1702:1710	arg1	β-Glucan					1688:1695	β-Glucan	1688:1695	β-Glucan	1688:1695	β-Glucan is a potential strategy for the treatment of AD by reshaping the gut microbiota and improving its metabolites.
36890624	10	55	theme	potential	1702:1710	arg1	strategy					1712:1719	a potential strategy	1700:1719	a potential strategy for the treatment of AD	1700:1743	β-Glucan is a potential strategy for the treatment of AD by reshaping the gut microbiota and improving its metabolites.
36890624	9	56	theme	AD	1601:1602	arg1	development					1586:1596	the development	1582:1596	the development of AD	1582:1602	CONCLUSION The imbalance of the gut microbiota and metabolites plays a role in the progression of AD; β-glucan blocks the development of AD by improving the gut microbiota and its metabolites and reducing neuroinflammation.
36890624	7	57	theme	inflammatory	1229:1240	arg1	factors					1242:1248	inflammatory factors	1229:1248	inflammatory factors	1229:1248	In addition, supplementation of β-glucan can also promote changes in the composition of the intestinal flora, thereby changing the flora metabolites in the intestinal content and reduce the activation of inflammatory factors and microglia in the cerebral cortex and hippocampus through the brain-gut axis.
36890624	0	58	theme	intestinal	72:81	arg1	flora					83:87	intestinal flora	72:87	intestinal flora	72:87	β-Glucan attenuates cognitive impairment of APP/PS1 mice via regulating intestinal flora and its metabolites.
36890624	8	59	theme	cerebral	1408:1415	arg1	cortex					1417:1422	cerebral cortex	1408:1422	cerebral cortex	1408:1422	While reducing the expression of inflammatory factors in the hippocampus and cerebral cortex, thereby controlling neuroinflammation.
36890624	3	60	theme	behavioral	465:474	arg1	testing					476:482	behavioral testing	465:482	behavioral testing	465:482	METHOD This study used behavioral testing to measure cognitive function.
36890624	6	61	theme	AD	955:956	arg1	progression					940:950	the progression	936:950	the progression of AD	936:956	RESULTS We found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition.
36890624	5	62	theme	factors	791:797	arg1	expressions					763:773	the expressions	759:773	the expressions of inflammatory factors in the mouse brain	759:816	Finally, the expressions of inflammatory factors in the mouse brain were detected by Western blot and Elisa methods.
36890624	4	63	used	used	584:587	arg2	GC-MS					573:577	GC-MS	573:577	GC-MS	573:577	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	4	63	used	used	584:587	arg2	sequencing					558:567	high-throughput 16 S rRNA gene sequencing	527:567	high-throughput 16 S rRNA gene sequencing	527:567	After that, high-throughput 16 S rRNA gene sequencing and GC-MS were used to analyze the intestinal microbiota and metabolite SCFAs of AD model mice, and further explore the relationship between intestinal flora and neuroinflammation.
36890624	1	64	dep	BACKGROUND	110:119	arg1	shown					151:155	shown	151:155	has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora	142:368	BACKGROUND The intestinal flora has been shown to be involved in the progression of Alzheimer's disease (AD) and can be improved by β-glucan, a polysaccharide derived from Saccharomyces cerevisiae, which affects cognitive function through the intestinal flora.
36890624	8	65	from	expression	1350:1359	arg1	hippocampus					1392:1402	the hippocampus	1388:1402	the hippocampus	1388:1402	While reducing the expression of inflammatory factors in the hippocampus and cerebral cortex, thereby controlling neuroinflammation.
36890624	8	65	from	expression	1350:1359	arg1	cortex					1417:1422	cerebral cortex	1408:1422	cerebral cortex	1408:1422	While reducing the expression of inflammatory factors in the hippocampus and cerebral cortex, thereby controlling neuroinflammation.
36890624	6	66	theme	cognitive	970:978	arg1	impairment					980:989	cognitive impairment	970:989	cognitive impairment	970:989	RESULTS We found that appropriate supplementation of β-glucan during the progression of AD can improve cognitive impairment and reduce A β plaque deposition.
35902013	7	0	theme	adsorption	863:872	arg1	experiments					874:884	The selective adsorption experiments	849:884	The selective adsorption experiments	849:884	The selective adsorption experiments showed that Cellulose1-ShMT3-CBM had a preference for Cd2+.
35902013	0	1	theme	friendly	102:109	arg1	biosorbent					111:120	an efficient and environmentally friendly biosorbent	69:120	an efficient and environmentally friendly biosorbent for Cd2+ removal	69:137	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	0	1	theme	friendly	102:109	arg1	material					57:64	composite material	47:64	composite material	47:64	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	6	2	theme	biosorbent	761:770	arg1	Cellulose1-ShMT3-CBM					772:791	The biosorbent Cellulose1-ShMT3-CBM	757:791	The biosorbent Cellulose1-ShMT3-CBM	757:791	The biosorbent Cellulose1-ShMT3-CBM was obtained by screening suitable cellulose materials.
35902013	10	3	theme	adsorption	1215:1224	arg1	efficiency					1226:1235	adsorption efficiency	1215:1235	adsorption efficiency	1215:1235	Regeneration experiments showed that adsorption efficiency was maintained after six cycles.
35902013	0	4	theme	Cd2+	126:129	arg1	removal					131:137	Cd2+ removal	126:137	Cd2+ removal	126:137	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	8	5	theme	removal	987:993	arg1	efficiency					995:1004	the removal efficiency	983:1004	the removal efficiency	983:1004	In low-concentration Cd2+ solutions, the removal efficiency was >99 %, and the adsorption equilibrium was reached within 15 min.
35902013	8	5	theme	removal	987:993	arg1	%					1014:1014	>99 %	1010:1014	>99 %	1010:1014	In low-concentration Cd2+ solutions, the removal efficiency was >99 %, and the adsorption equilibrium was reached within 15 min.
35902013	13	6	theme	actual	1567:1572	arg1	treatment					1580:1588	actual water treatment	1567:1588	actual water treatment	1567:1588	In this study, an environmentally friendly biosorbent which can adsorb Cd2+ efficiently and quickly was prepared for actual water treatment.
35902013	8	7	theme	Cd2+	967:970	arg1	solutions					972:980	low-concentration Cd2+ solutions	949:980	low-concentration Cd2+ solutions	949:980	In low-concentration Cd2+ solutions, the removal efficiency was >99 %, and the adsorption equilibrium was reached within 15 min.
35902013	3	8	theme	Cd	327:328	arg1	MT					323:324	MT	323:324	MT (Cd specificity)	323:341	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	3	8	theme	Cd	327:328	arg1	specificity					330:340	Cd specificity	327:340	Cd specificity	327:340	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	3	9	from	crab	359:362	arg1	cDNA					315:318	the full-length cDNA	299:318	the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT)	299:393	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	13	10	theme	water	1574:1578	arg1	treatment					1580:1588	actual water treatment	1567:1588	actual water treatment	1567:1588	In this study, an environmentally friendly biosorbent which can adsorb Cd2+ efficiently and quickly was prepared for actual water treatment.
35902013	1	11	theme	cysteine-rich	178:190	arg1	proteins					206:213	cysteine-rich metal-binding proteins	178:213	cysteine-rich metal-binding proteins	178:213	Metallothioneins (MTs) are a class of cysteine-rich metal-binding proteins.
35902013	8	12	theme	adsorption	1025:1034	arg1	equilibrium					1036:1046	the adsorption equilibrium	1021:1046	the adsorption equilibrium	1021:1046	In low-concentration Cd2+ solutions, the removal efficiency was >99 %, and the adsorption equilibrium was reached within 15 min.
35902013	7	13	contain	had	919:921	arg2	preference					925:934	a preference	923:934	a preference for Cd2+	923:943	The selective adsorption experiments showed that Cellulose1-ShMT3-CBM had a preference for Cd2+.
35902013	7	13	contain	had	919:921	arg1	Cellulose1-ShMT3-CBM					898:917	Cellulose1-ShMT3-CBM	898:917	Cellulose1-ShMT3-CBM	898:917	The selective adsorption experiments showed that Cellulose1-ShMT3-CBM had a preference for Cd2+.
35902013	1	14	theme	metal-binding	192:204	arg1	proteins					206:213	cysteine-rich metal-binding proteins	178:213	cysteine-rich metal-binding proteins	178:213	Metallothioneins (MTs) are a class of cysteine-rich metal-binding proteins.
35902013	0	15	theme	environmentally	86:100	arg1	biosorbent					111:120	an efficient and environmentally friendly biosorbent	69:120	an efficient and environmentally friendly biosorbent for Cd2+ removal	69:137	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	0	15	theme	environmentally	86:100	arg1	material					57:64	composite material	47:64	composite material	47:64	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	12	16	theme	water	1417:1421	arg1	samples					1423:1429	actual water samples	1410:1429	actual water samples	1410:1429	Meanwhile, Cellulose1-ShMT3-CBM can preferentially remove Cd2+ in actual water samples and boiler sewage.
35902013	11	17	theme	MTT	1274:1276	arg1	experiment					1278:1287	The MTT experiment	1270:1287	The MTT experiment	1270:1287	The MTT experiment showed that Cellulose1-ShMT3-CBM had low cytotoxicity.
35902013	1	18	theme	proteins	206:213	arg1	Metallothioneins					140:155	Metallothioneins	140:155	Metallothioneins (MTs)	140:161	Metallothioneins (MTs) are a class of cysteine-rich metal-binding proteins.
35902013	1	18	theme	proteins	206:213	arg1	class					169:173	a class	167:173	a class of cysteine-rich metal-binding proteins	167:213	Metallothioneins (MTs) are a class of cysteine-rich metal-binding proteins.
35902013	3	19	theme	Sinopotamon	365:375	arg1	crab					359:362	freshwater crab	348:362	freshwater crab (Sinopotamon henanense) (ShMT)	348:393	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	3	19	theme	Sinopotamon	365:375	arg1	henanense					377:385	Sinopotamon henanense	365:385	Sinopotamon henanense	365:385	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	11	20	theme	low	1326:1328	arg1	cytotoxicity					1330:1341	low cytotoxicity	1326:1341	low cytotoxicity	1326:1341	The MTT experiment showed that Cellulose1-ShMT3-CBM had low cytotoxicity.
35902013	12	21	theme	actual	1410:1415	arg1	samples					1423:1429	actual water samples	1410:1429	actual water samples	1410:1429	Meanwhile, Cellulose1-ShMT3-CBM can preferentially remove Cd2+ in actual water samples and boiler sewage.
35902013	9	22	theme	saturated	1079:1087	arg1	capacity					1100:1107	The saturated adsorption capacity	1075:1107	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+	1075:1140	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	9	22	theme	saturated	1079:1087	arg1	180.35 ± 4.67 mg/g					1145:1162	180.35 ± 4.67 mg/g	1145:1162	180.35 ± 4.67 mg/g (Dry Weight)	1145:1175	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	4	23	theme	fusion	611:616	arg1	ShMT3-CBM					580:588	ShMT3-CBM	580:588	ShMT3-CBM	580:588	Here, ShMT3-CBM, a novel recombinant fusion protein, was prepared.
35902013	4	23	theme	fusion	611:616	arg1	protein					618:624	a novel recombinant fusion protein	591:624	a novel recombinant fusion protein	591:624	Here, ShMT3-CBM, a novel recombinant fusion protein, was prepared.
35902013	3	24	theme	freshwater	348:357	arg1	crab					359:362	freshwater crab	348:362	freshwater crab (Sinopotamon henanense) (ShMT)	348:393	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	3	24	theme	freshwater	348:357	arg1	henanense					377:385	Sinopotamon henanense	365:385	Sinopotamon henanense	365:385	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	3	24	theme	freshwater	348:357	arg1	ShMT					389:392	ShMT	389:392	ShMT	389:392	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	11	25	contain	had	1322:1324	arg2	cytotoxicity					1330:1341	low cytotoxicity	1326:1341	low cytotoxicity	1326:1341	The MTT experiment showed that Cellulose1-ShMT3-CBM had low cytotoxicity.
35902013	11	25	contain	had	1322:1324	arg1	Cellulose1-ShMT3-CBM					1301:1320	Cellulose1-ShMT3-CBM	1301:1320	Cellulose1-ShMT3-CBM	1301:1320	The MTT experiment showed that Cellulose1-ShMT3-CBM had low cytotoxicity.
35902013	9	26	theme	adsorption	1089:1098	arg1	capacity					1100:1107	The saturated adsorption capacity	1075:1107	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+	1075:1140	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	9	26	theme	adsorption	1089:1098	arg1	180.35 ± 4.67 mg/g					1145:1162	180.35 ± 4.67 mg/g	1145:1162	180.35 ± 4.67 mg/g (Dry Weight)	1145:1175	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	12	27	from	Cd2+	1402:1405	arg1	samples					1423:1429	actual water samples	1410:1429	actual water samples	1410:1429	Meanwhile, Cellulose1-ShMT3-CBM can preferentially remove Cd2+ in actual water samples and boiler sewage.
35902013	3	28	theme	previous	280:287	arg1	research					289:296	our previous research	276:296	our previous research	276:296	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	8	29	from	%	1014:1014	arg1	solutions					972:980	low-concentration Cd2+ solutions	949:980	low-concentration Cd2+ solutions	949:980	In low-concentration Cd2+ solutions, the removal efficiency was >99 %, and the adsorption equilibrium was reached within 15 min.
35902013	6	30	theme	cellulose	828:836	arg1	materials					838:846	suitable cellulose materials	819:846	suitable cellulose materials	819:846	The biosorbent Cellulose1-ShMT3-CBM was obtained by screening suitable cellulose materials.
35902013	4	31	theme	recombinant	599:609	arg1	ShMT3-CBM					580:588	ShMT3-CBM	580:588	ShMT3-CBM	580:588	Here, ShMT3-CBM, a novel recombinant fusion protein, was prepared.
35902013	4	31	theme	recombinant	599:609	arg1	protein					618:624	a novel recombinant fusion protein	591:624	a novel recombinant fusion protein	591:624	Here, ShMT3-CBM, a novel recombinant fusion protein, was prepared.
35902013	5	32	theme	carbohydrate	650:661	arg1	module					671:676	a carbohydrate binding module	648:676	a carbohydrate binding module that can specifically bind cellulose while ShMT3 can effectively chelate Cd2+	648:754	CBM is a carbohydrate binding module that can specifically bind cellulose while ShMT3 can effectively chelate Cd2+.
35902013	5	32	theme	carbohydrate	650:661	arg1	CBM					641:643	CBM	641:643	CBM	641:643	CBM is a carbohydrate binding module that can specifically bind cellulose while ShMT3 can effectively chelate Cd2+.
35902013	6	33	theme	suitable	819:826	arg1	materials					838:846	suitable cellulose materials	819:846	suitable cellulose materials	819:846	The biosorbent Cellulose1-ShMT3-CBM was obtained by screening suitable cellulose materials.
35902013	0	34	theme	composite	47:55	arg1	biosorbent					111:120	an efficient and environmentally friendly biosorbent	69:120	an efficient and environmentally friendly biosorbent for Cd2+ removal	69:137	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	0	34	theme	composite	47:55	arg1	material					57:64	composite material	47:64	composite material	47:64	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	9	35	theme	Cellulose1-ShMT3-CBM	1112:1131	arg1	capacity					1100:1107	The saturated adsorption capacity	1075:1107	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+	1075:1140	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	9	35	theme	Cellulose1-ShMT3-CBM	1112:1131	arg1	180.35 ± 4.67 mg/g					1145:1162	180.35 ± 4.67 mg/g	1145:1162	180.35 ± 4.67 mg/g (Dry Weight)	1145:1175	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	0	36	theme	efficient	72:80	arg1	biosorbent					111:120	an efficient and environmentally friendly biosorbent	69:120	an efficient and environmentally friendly biosorbent for Cd2+ removal	69:137	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	0	36	theme	efficient	72:80	arg1	material					57:64	composite material	47:64	composite material	47:64	Genetically modified metallothionein/cellulose composite material as an efficient and environmentally friendly biosorbent for Cd2+ removal.
35902013	5	37	theme	binding	663:669	arg1	module					671:676	a carbohydrate binding module	648:676	a carbohydrate binding module that can specifically bind cellulose while ShMT3 can effectively chelate Cd2+	648:754	CBM is a carbohydrate binding module that can specifically bind cellulose while ShMT3 can effectively chelate Cd2+.
35902013	5	37	theme	binding	663:669	arg1	CBM					641:643	CBM	641:643	CBM	641:643	CBM is a carbohydrate binding module that can specifically bind cellulose while ShMT3 can effectively chelate Cd2+.
35902013	3	38	theme	actual	531:536	arg1	adsorption					543:552	actual Cd2+ adsorption	531:552	actual Cd2+ adsorption due to instability	531:571	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	13	39	theme	environmentally	1468:1482	arg1	biosorbent					1493:1502	an environmentally friendly biosorbent	1465:1502	an environmentally friendly biosorbent which can adsorb Cd2+ efficiently and quickly	1465:1548	In this study, an environmentally friendly biosorbent which can adsorb Cd2+ efficiently and quickly was prepared for actual water treatment.
35902013	3	40	theme	full-length	303:313	arg1	cDNA					315:318	the full-length cDNA	299:318	the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT)	299:393	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	3	41	theme	Cd2+	538:541	arg1	adsorption					543:552	actual Cd2+ adsorption	531:552	actual Cd2+ adsorption due to instability	531:571	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	13	42	theme	friendly	1484:1491	arg1	biosorbent					1493:1502	an environmentally friendly biosorbent	1465:1502	an environmentally friendly biosorbent which can adsorb Cd2+ efficiently and quickly	1465:1548	In this study, an environmentally friendly biosorbent which can adsorb Cd2+ efficiently and quickly was prepared for actual water treatment.
35902013	2	43	theme	pollutants	261:270	arg1	one					232:234	one	232:234	one	232:234	Cadmium (Cd) is one of the toxic heavy metal pollutants.
35902013	2	43	theme	pollutants	261:270	arg1	pollutants					261:270	the toxic heavy metal pollutants	239:270	the toxic heavy metal pollutants	239:270	Cadmium (Cd) is one of the toxic heavy metal pollutants.
35902013	9	44	theme	Dry	1165:1167	arg1	180.35 ± 4.67 mg/g					1145:1162	180.35 ± 4.67 mg/g	1145:1162	180.35 ± 4.67 mg/g (Dry Weight)	1145:1175	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	9	44	theme	Dry	1165:1167	arg1	Weight					1169:1174	Dry Weight	1165:1174	Dry Weight	1165:1174	The saturated adsorption capacity of Cellulose1-ShMT3-CBM for Cd2+ is 180.35 ± 4.67 mg/g (Dry Weight).
35902013	2	45	theme	metal	255:259	arg1	pollutants					261:270	the toxic heavy metal pollutants	239:270	the toxic heavy metal pollutants	239:270	Cadmium (Cd) is one of the toxic heavy metal pollutants.
35902013	3	46	theme	site-directed	443:455	arg1	mutagenesis					457:467	site-directed mutagenesis	443:467	site-directed mutagenesis	443:467	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	10	47	theme	Regeneration	1178:1189	arg1	experiments					1191:1201	Regeneration experiments	1178:1201	Regeneration experiments	1178:1201	Regeneration experiments showed that adsorption efficiency was maintained after six cycles.
35902013	3	48	theme	MT	323:324	arg1	cDNA					315:318	the full-length cDNA	299:318	the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT)	299:393	In our previous research, the full-length cDNA of MT (Cd specificity) from freshwater crab (Sinopotamon henanense) (ShMT) was cloned and genetically modified to ShMT3 by site-directed mutagenesis to enhance the tolerance for Cd2+, however, it was limited in actual Cd2+ adsorption due to instability.
35902013	2	49	theme	heavy	249:253	arg1	pollutants					261:270	the toxic heavy metal pollutants	239:270	the toxic heavy metal pollutants	239:270	Cadmium (Cd) is one of the toxic heavy metal pollutants.
35902013	4	50	theme	novel	593:597	arg1	ShMT3-CBM					580:588	ShMT3-CBM	580:588	ShMT3-CBM	580:588	Here, ShMT3-CBM, a novel recombinant fusion protein, was prepared.
35902013	4	50	theme	novel	593:597	arg1	protein					618:624	a novel recombinant fusion protein	591:624	a novel recombinant fusion protein	591:624	Here, ShMT3-CBM, a novel recombinant fusion protein, was prepared.
35902013	2	51	theme	toxic	243:247	arg1	pollutants					261:270	the toxic heavy metal pollutants	239:270	the toxic heavy metal pollutants	239:270	Cadmium (Cd) is one of the toxic heavy metal pollutants.
35902013	8	52	theme	low-concentration	949:965	arg1	solutions					972:980	low-concentration Cd2+ solutions	949:980	low-concentration Cd2+ solutions	949:980	In low-concentration Cd2+ solutions, the removal efficiency was >99 %, and the adsorption equilibrium was reached within 15 min.
35902013	7	53	theme	selective	853:861	arg1	experiments					874:884	The selective adsorption experiments	849:884	The selective adsorption experiments	849:884	The selective adsorption experiments showed that Cellulose1-ShMT3-CBM had a preference for Cd2+.
36292917	0	0	theme	Bioactivated	82:93	arg1	Chitosan					95:102	Bioactivated Chitosan	82:102	Bioactivated Chitosan	82:102	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	6	1	theme	maximum	903:909	arg1	stress					911:916	maximum stress	903:916	maximum stress	903:916	Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress.
36292917	10	2	theme	OxPVA	1610:1614	arg1	bioactivity					1624:1634	OxPVA certain bioactivity	1610:1634	OxPVA certain bioactivity	1610:1634	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	8	3	theme	OxPVA/ChS+EAK	1321:1333	arg1	scaffolds					1335:1343	OxPVA/ChS+EAK scaffolds	1321:1343	OxPVA/ChS+EAK scaffolds	1321:1343	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	2	4	with	coupling	284:291	arg1	sheath					311:316	a natural sheath	301:316	a natural sheath	301:316	Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity.
36292917	8	5	theme	+EAK	1149:1152	arg1	groups					1154:1159	both the OxPVA/ChS and +EAK groups	1126:1159	groups	1154:1159	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	10	6	theme	appealing	1665:1673	arg1	NCs					1675:1677	future appealing NCs	1658:1677	future appealing NCs	1658:1677	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	4	7	theme	self-assembling-peptide	537:559	arg1	sequences					605:613	the laminin-derived sequences	585:613	without/with the laminin-derived sequences -IKVAV/-YIGSR	572:627	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	4	7	theme	self-assembling-peptide	537:559	arg1	EAK					567:569	the self-assembling-peptide (SAP) EAK	533:569	the self-assembling-peptide (SAP) EAK	533:569	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	10	8	theme	IKVAV/YIGSR	1576:1586	arg1	motifs					1588:1593	IKVAV/YIGSR motifs	1576:1593	IKVAV/YIGSR motifs	1576:1593	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	6	9	theme	modulus	891:897	arg1	values					869:874	the highest values	857:874	the highest values of compressive modulus and maximum stress	857:916	Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress.
36292917	10	10	theme	neuro-friendly	1539:1552	arg1	attitude					1554:1561	neuro-friendly attitude	1539:1561	neuro-friendly attitude	1539:1561	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	0	11	theme	Chitosan	95:102	arg1	Sponges					104:110	Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges	51:110	Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes	51:142	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	6	12	theme	compressive	879:889	arg1	modulus					891:897	compressive modulus	879:897	compressive modulus	879:897	Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress.
36292917	7	13	theme	+EAK	934:937	arg1	groups					939:944	+EAK groups	934:944	+EAK groups	934:944	However, among +EAK groups, ChS+EAK showed a significantly higher maximum stress than that found for ChS+EAK-IKVAV and ChS+EAK-YIGSR.
36292917	2	14	theme	device	260:265	arg1	optimization					267:278	OxPVA device optimization	254:278	OxPVA device optimization	254:278	Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity.
36292917	4	15	with	interaction	480:490	arg1	cells					497:501	cells	497:501	cells	497:501	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	4	16	theme	SAP	562:564	arg1	sequences					605:613	the laminin-derived sequences	585:613	without/with the laminin-derived sequences -IKVAV/-YIGSR	572:627	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	4	16	theme	SAP	562:564	arg1	EAK					567:569	the self-assembling-peptide (SAP) EAK	533:569	the self-assembling-peptide (SAP) EAK	533:569	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	0	17	theme	Tissue	116:121	arg1	Purposes					135:142	Tissue Engineering Purposes	116:142	Tissue Engineering Purposes	116:142	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	3	18	theme	two-layered	450:460	arg1	NCs					462:464	two-layered NCs	450:464	two-layered NCs	450:464	Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs.
36292917	4	19	theme	laminin-derived	589:603	arg1	sequences					605:613	the laminin-derived sequences	585:613	without/with the laminin-derived sequences -IKVAV/-YIGSR	572:627	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	4	19	theme	laminin-derived	589:603	arg1	EAK					567:569	the self-assembling-peptide (SAP) EAK	533:569	the self-assembling-peptide (SAP) EAK	533:569	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	2	20	theme	OxPVA	254:258	arg1	optimization					267:278	OxPVA device optimization	254:278	OxPVA device optimization	254:278	Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity.
36292917	9	21	theme	subcutaneous	1437:1448	arg1	implantation					1450:1461	subcutaneous implantation	1437:1461	subcutaneous implantation in Balb/c mice	1437:1476	The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility.
36292917	5	22	theme	Scanning	727:734	arg1	Microscopy					745:754	Scanning Electron Microscopy	727:754	Scanning Electron Microscopy	727:754	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	5	22	theme	Scanning	727:734	arg1	SEM					757:759	SEM	757:759	SEM	757:759	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	9	23	theme	slight	1400:1405	arg1	inflammation					1407:1418	only a slight inflammation	1393:1418	only a slight inflammation	1393:1418	The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility.
36292917	6	24	theme	highest	861:867	arg1	values					869:874	the highest values	857:874	the highest values of compressive modulus and maximum stress	857:916	Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress.
36292917	5	25	theme	Electron	736:743	arg1	Microscopy					745:754	Scanning Electron Microscopy	727:754	Scanning Electron Microscopy	727:754	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	5	25	theme	Electron	736:743	arg1	SEM					757:759	SEM	757:759	SEM	757:759	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	10	26	theme	EAK	1519:1521	arg1	features					1526:1533	EAK 3D features	1519:1533	EAK 3D features	1519:1533	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	8	27	theme	significant	1251:1261	arg1	differences					1263:1273	significant differences	1251:1273	significant differences from day 7	1251:1284	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	10	28	theme	ChS	1505:1507	arg1	porosity					1509:1516	ChS porosity	1505:1516	ChS porosity	1505:1516	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	1	29	theme	new	185:187	arg1	alcohol					164:170	Oxidized polyvinyl alcohol	145:170	Oxidized polyvinyl alcohol (OxPVA)	145:178	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	1	29	theme	new	185:187	arg1	polymer					189:195	a new polymer	183:195	a new polymer for the fabrication of nerve conduits (NCs)	183:239	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	0	30	theme	Hybrid	25:30	arg1	Scaffolds					32:40	Two-Layer Hybrid Scaffolds	15:40	Two-Layer Hybrid Scaffolds	15:40	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	8	31	theme	total	1299:1303	arg1	number					1310:1315	a higher total cell number	1290:1315	a higher total cell number for OxPVA/ChS+EAK scaffolds	1290:1343	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	8	32	theme	microporous	1081:1091	arg1	interconnections					1093:1108	microporous interconnections	1081:1108	microporous interconnections	1081:1108	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	9	33	theme	Balb/c	1466:1471	arg1	mice					1473:1476	Balb/c mice	1466:1476	Balb/c mice	1466:1476	The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility.
36292917	3	34	theme	OxPVA/chitosan	347:360	arg1	sponges					362:368	OxPVA/chitosan sponges	347:368	OxPVA/chitosan sponges (ChS) as hybrid scaffolds	347:394	Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs.
36292917	3	34	theme	OxPVA/chitosan	347:360	arg1	ChS					371:373	ChS	371:373	ChS	371:373	Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs.
36292917	0	35	theme	Engineering	123:133	arg1	Purposes					135:142	Tissue Engineering Purposes	116:142	Tissue Engineering Purposes	116:142	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	8	36	from	day	1280:1282	arg1	number					1310:1315	a higher total cell number	1290:1315	a higher total cell number for OxPVA/ChS+EAK scaffolds	1290:1343	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	8	36	from	day	1280:1282	arg1	differences					1263:1273	significant differences	1251:1273	significant differences from day 7	1251:1284	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	3	37	theme	vivo	432:435	arg1	behaviour					437:445	the vivo behaviour	428:445	the vivo behaviour of two-layered NCs	428:464	Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs.
36292917	8	38	theme	SH-SY5Y	1207:1213	arg1	cells					1215:1219	SH-SY5Y cells	1207:1219	SH-SY5Y cells' adhesion/proliferation	1207:1243	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	5	39	theme	mechanical	688:697	arg1	properties					699:708	mechanical properties	688:708	mechanical properties	688:708	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	9	40	theme	implantation	1450:1461	arg1	days					1429:1432	14 days	1426:1432	14 days of subcutaneous implantation in Balb/c mice	1426:1476	The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility.
36292917	10	41	theme	future	1658:1663	arg1	NCs					1675:1677	future appealing NCs	1658:1677	future appealing NCs	1658:1677	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	0	42	theme	Scaffolds	32:40	arg1	Development					0:10	Development	0:10	Development of Two-Layer Hybrid Scaffolds	0:40	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	3	43	theme	hybrid	379:384	arg1	scaffolds					386:394	hybrid scaffolds	379:394	hybrid scaffolds	379:394	Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs.
36292917	1	44	theme	conduits	226:233	arg1	fabrication					205:215	the fabrication	201:215	the fabrication of nerve conduits (NCs)	201:239	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	9	45	from	mice	1473:1476	arg1	days					1429:1432	14 days	1426:1432	14 days of subcutaneous implantation in Balb/c mice	1426:1476	The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility.
36292917	8	46	theme	higher	1292:1297	arg1	number					1310:1315	a higher total cell number	1290:1315	a higher total cell number for OxPVA/ChS+EAK scaffolds	1290:1343	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	0	47	theme	Oxidized	51:58	arg1	Sponges					104:110	Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges	51:110	Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes	51:142	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	10	48	theme	3D	1523:1524	arg1	features					1526:1533	EAK 3D features	1519:1533	EAK 3D features	1519:1533	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	6	49	theme	peptide-free	833:844	arg1	ChS					846:848	the peptide-free ChS	829:848	the peptide-free ChS	829:848	Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress.
36292917	2	50	dep	coupling	284:291	arg1	it					293:294	it	293:294	it	293:294	Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity.
36292917	5	51	theme	hybrid	648:653	arg1	scaffolds					655:663	the hybrid scaffolds	644:663	the hybrid scaffolds	644:663	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	7	52	theme	maximum	985:991	arg1	stress					993:998	a significantly higher maximum stress	962:998	a significantly higher maximum stress	962:998	However, among +EAK groups, ChS+EAK showed a significantly higher maximum stress than that found for ChS+EAK-IKVAV and ChS+EAK-YIGSR.
36292917	3	53	theme	NCs	462:464	arg1	behaviour					437:445	the vivo behaviour	428:445	the vivo behaviour of two-layered NCs	428:464	Thus, OxPVA/chitosan sponges (ChS) as hybrid scaffolds were investigated to predict in the vivo behaviour of two-layered NCs.
36292917	4	54	link	laminin-derived	589:603	arg1	sequences					605:613	the laminin-derived sequences	585:613	without/with the laminin-derived sequences -IKVAV/-YIGSR	572:627	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	4	54	link	laminin-derived	589:603	arg1	EAK					567:569	the self-assembling-peptide (SAP) EAK	533:569	the self-assembling-peptide (SAP) EAK	533:569	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	9	55	from	implantation	1450:1461	arg1	mice					1473:1476	Balb/c mice	1466:1476	Balb/c mice	1466:1476	The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility.
36292917	2	56	theme	natural	303:309	arg1	sheath					311:316	a natural sheath	301:316	a natural sheath	301:316	Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity.
36292917	5	57	dep	ultrastructure	711:724	arg1	Microscopy					745:754	Scanning Electron Microscopy	727:754	Scanning Electron Microscopy	727:754	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	5	57	dep	ultrastructure	711:724	arg1	SEM					757:759	SEM	757:759	SEM	757:759	Thus, ChS and the hybrid scaffolds were characterized for mechanical properties, ultrastructure (Scanning Electron Microscopy, SEM), bioactivity, and biocompatibility.
36292917	1	58	theme	Oxidized	145:152	arg1	alcohol					164:170	Oxidized polyvinyl alcohol	145:170	Oxidized polyvinyl alcohol (OxPVA)	145:178	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	1	58	theme	Oxidized	145:152	arg1	polymer					189:195	a new polymer	183:195	a new polymer for the fabrication of nerve conduits (NCs)	183:239	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	1	58	theme	Oxidized	145:152	arg1	OxPVA					173:177	OxPVA	173:177	OxPVA	173:177	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	8	59	theme	OxPVA/ChS	1135:1143	arg1	groups					1154:1159	both the OxPVA/ChS and +EAK groups	1126:1159	groups	1154:1159	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	1	60	theme	nerve	220:224	arg1	conduits					226:233	nerve conduits	220:233	nerve conduits (NCs)	220:239	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	1	60	theme	nerve	220:224	arg1	NCs					236:238	NCs	236:238	NCs	236:238	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	0	61	theme	Alcohol	70:76	arg1	Sponges					104:110	Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges	51:110	Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes	51:142	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	6	62	theme	mechanical	808:817	arg1	analysis					819:826	mechanical analysis	808:826	mechanical analysis	808:826	Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress.
36292917	7	63	theme	higher	978:983	arg1	stress					993:998	a significantly higher maximum stress	962:998	a significantly higher maximum stress	962:998	However, among +EAK groups, ChS+EAK showed a significantly higher maximum stress than that found for ChS+EAK-IKVAV and ChS+EAK-YIGSR.
36292917	1	64	theme	polyvinyl	154:162	arg1	alcohol					164:170	Oxidized polyvinyl alcohol	145:170	Oxidized polyvinyl alcohol (OxPVA)	145:178	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	1	64	theme	polyvinyl	154:162	arg1	polymer					189:195	a new polymer	183:195	a new polymer for the fabrication of nerve conduits (NCs)	183:239	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	1	64	theme	polyvinyl	154:162	arg1	OxPVA					173:177	OxPVA	173:177	OxPVA	173:177	Oxidized polyvinyl alcohol (OxPVA) is a new polymer for the fabrication of nerve conduits (NCs).
36292917	2	65	with	optimization	267:278	arg1	sheath					311:316	a natural sheath	301:316	a natural sheath	301:316	Looking for OxPVA device optimization and coupling it with a natural sheath may boost bioactivity.
36292917	0	66	theme	Polyvinyl	60:68	arg1	Alcohol					70:76	Polyvinyl Alcohol	60:76	Polyvinyl Alcohol	60:76	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	6	67	theme	stress	911:916	arg1	values					869:874	the highest values	857:874	the highest values of compressive modulus and maximum stress	857:916	Regarding mechanical analysis, the peptide-free ChS showed the highest values of compressive modulus and maximum stress.
36292917	0	68	theme	Two-Layer	15:23	arg1	Scaffolds					32:40	Two-Layer Hybrid Scaffolds	15:40	Two-Layer Hybrid Scaffolds	15:40	Development of Two-Layer Hybrid Scaffolds Based on Oxidized Polyvinyl Alcohol and Bioactivated Chitosan Sponges for Tissue Engineering Purposes.
36292917	9	69	from	days	1429:1432	arg1	mice					1473:1476	Balb/c mice	1466:1476	Balb/c mice	1466:1476	The scaffolds elicited only a slight inflammation after 14 days of subcutaneous implantation in Balb/c mice, proving biocompatibility.
36292917	8	70	theme	cell	1305:1308	arg1	number					1310:1315	a higher total cell number	1290:1315	a higher total cell number for OxPVA/ChS+EAK scaffolds	1290:1343	Considering ultrastructure, microporous interconnections were tighter in both the OxPVA/ChS and +EAK groups than in the others; all the scaffolds induced SH-SY5Y cells' adhesion/proliferation, with significant differences from day 7 and a higher total cell number for OxPVA/ChS+EAK scaffolds, in accordance with SEM.
36292917	10	71	theme	certain	1616:1622	arg1	bioactivity					1624:1634	OxPVA certain bioactivity	1610:1634	OxPVA certain bioactivity	1610:1634	ChS porosity, EAK 3D features and neuro-friendly attitude (shared with IKVAV/YIGSR motifs) may confer to OxPVA certain bioactivity, laying the basis for future appealing NCs.
36292917	4	72	theme	without/with	572:583	arg1	sequences					605:613	the laminin-derived sequences	585:613	without/with the laminin-derived sequences -IKVAV/-YIGSR	572:627	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
36292917	4	72	theme	without/with	572:583	arg1	EAK					567:569	the self-assembling-peptide (SAP) EAK	533:569	the self-assembling-peptide (SAP) EAK	533:569	To encourage interaction with cells, ChS were functionalized with the self-assembling-peptide (SAP) EAK, without/with the laminin-derived sequences -IKVAV/-YIGSR.
35164370	7	0	theme	slow	1067:1070	arg1	release					1072:1078	the slow release	1063:1078	the slow release of AmpB from PB	1063:1094	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	7	0	theme	slow	1067:1070	arg1	unusual					1100:1106	unusual	1100:1106	unusual	1100:1106	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	6	1	theme	free	792:795	arg1	AmpB					797:800	the free AmpB	788:800	the free AmpB	788:800	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	3	2	theme	proteolytic	470:480	arg1	inhibitors					482:491	proteolytic inhibitors	470:491	proteolytic inhibitors	470:491	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	9	3	used	used	1527:1530	arg2	PB/liposome-PB					1503:1516	PB/liposome-PB	1503:1516	PB/liposome-PB	1503:1516	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	9	3	used	used	1527:1530	arg2	systems					1563:1569	safe and effective delivery systems	1535:1569	safe and effective delivery systems	1535:1569	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	4	4	theme	In	564:565	arg1	efficacy					573:580	In vitro efficacy	564:580	In vitro efficacy of the different formulations	564:610	In vitro efficacy of the different formulations was examined.
35164370	7	5	with	interaction	1185:1195	arg1	proteins					1209:1216	plasma proteins	1202:1216	plasma proteins	1202:1216	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	3	6	theme	linkers	500:506	arg1	use					427:429	the use	423:429	the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations	423:561	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	2	7	theme	drugs	280:284	arg1	earlier					205:211	earlier	205:211	earlier	205:211	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	2	7	theme	drugs	280:284	arg1	carrier					269:275	an efficient carrier	256:275	an efficient carrier of drugs and antigens	256:297	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	1	8	theme	tissue	170:175	arg1	engineering					177:187	tissue engineering	170:187	drug and gene delivery as well as tissue engineering	136:187	Fibrin-based systems offer promises in drug and gene delivery as well as tissue engineering.
35164370	4	9	theme	formulations	599:610	arg1	efficacy					573:580	In vitro efficacy	564:580	In vitro efficacy of the different formulations	564:610	In vitro efficacy of the different formulations was examined.
35164370	4	10	dep	In	564:565	arg1	vitro					567:571	vitro	567:571	vitro	567:571	In vitro efficacy of the different formulations was examined.
35164370	3	11	theme	entrapment	518:527	arg1	use					427:429	the use	423:429	the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations	423:561	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	2	12	theme	fibrin-based	215:226	arg1	PB					242:243	PB	242:243	PB	242:243	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	2	12	theme	fibrin-based	215:226	arg1	beads					235:239	fibrin-based plasma beads	215:239	earlier a fibrin-based plasma beads (PB) system	205:251	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	6	13	theme	AmpB	906:909	arg1	release					911:917	AmpB release	906:917	AmpB release in the circulation (>144 h)	906:945	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	6	13	theme	AmpB	906:909	arg1	h					944:944	>144 h	939:944	>144 h	939:944	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	7	14	from	molecules	1132:1140	arg1	findings					1157:1164	our earlier findings	1145:1164	our earlier findings	1145:1164	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	8	15	theme	interaction	1229:1239	arg1	studies					1241:1247	Molecular interaction studies	1219:1247	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking	1219:1383	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	8	16	dep	in	1367:1368	arg1	silico					1370:1375	silico	1370:1375	silico	1370:1375	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	8	17	theme	serum	1259:1263	arg1	albumin					1265:1271	bovine serum albumin	1252:1271	bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking	1252:1383	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	6	18	dep	effective	882:890	arg1	compared					1002:1009	compared	1002:1009	compared to the liposomal formulations alone	1002:1045	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	2	19	theme	beads	235:239	arg1	system					246:251	a fibrin-based plasma beads (PB) system	213:251	earlier a fibrin-based plasma beads (PB) system	205:251	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	8	20	theme	AmpB	1443:1446	arg1	release					1432:1438	the slow release	1423:1438	the slow release of AmpB entrapped in PB alone	1423:1468	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	5	21	theme	Swiss	745:749	arg1	mice					758:761	Swiss albino mice	745:761	Swiss albino mice	745:761	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	9	22	theme	above	1475:1479	arg1	findings					1481:1488	The above findings	1471:1488	The above findings	1471:1488	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	0	23	theme	Fungal	78:83	arg1	Infections					85:94	Combat Fungal Infections	71:94	Combat Fungal Infections	71:94	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	3	24	theme	present	307:313	arg1	work					315:318	the present work	303:318	the present work	303:318	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	7	25	theme	other	1120:1124	arg1	molecules					1132:1140	other small molecules	1120:1140	other small molecules in our earlier findings	1120:1164	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	5	26	theme	albino	751:756	arg1	mice					758:761	Swiss albino mice	745:761	Swiss albino mice	745:761	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	0	27	theme	Plasma	0:5	arg1	Liposomes					22:30	Plasma Bead Entrapped Liposomes	0:30	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.	0:95	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	0	28	theme	Entrapped	12:20	arg1	Liposomes					22:30	Plasma Bead Entrapped Liposomes	0:30	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.	0:95	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	1	29	theme	Fibrin-based	97:108	arg1	systems					110:116	Fibrin-based systems	97:116	Fibrin-based systems	97:116	Fibrin-based systems offer promises in drug and gene delivery as well as tissue engineering.
35164370	3	30	theme	therapeutic	363:373	arg1	efficacy					375:382	its therapeutic efficacy	359:382	its therapeutic efficacy	359:382	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	8	31	theme	titration	1341:1349	arg1	calorimetry					1351:1361	isothermal titration calorimetry	1330:1361	isothermal titration calorimetry	1330:1361	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	0	32	theme	Delivery	52:59	arg1	System					61:66	a Potential Drug Delivery System	35:66	a Potential Drug Delivery System to Combat Fungal Infections	35:94	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	3	33	theme	composite	450:458	arg1	matrices					460:467	plasma alginate composite matrices	434:467	plasma alginate composite matrices	434:467	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	0	34	theme	Potential	37:45	arg1	System					61:66	a Potential Drug Delivery System	35:66	a Potential Drug Delivery System to Combat Fungal Infections	35:94	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	9	35	theme	delivery	1554:1561	arg1	PB/liposome-PB					1503:1516	PB/liposome-PB	1503:1516	PB/liposome-PB	1503:1516	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	9	35	theme	delivery	1554:1561	arg1	systems					1563:1569	safe and effective delivery systems	1535:1569	safe and effective delivery systems	1535:1569	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	6	36	from	release	911:917	arg1	organs					989:994	the vital organs	979:994	the vital organs	979:994	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	6	36	from	release	911:917	arg1	circulation					926:936	the circulation	922:936	the circulation	922:936	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	3	37	theme	plasma	434:439	arg1	matrices					460:467	plasma alginate composite matrices	434:467	plasma alginate composite matrices	434:467	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	7	38	theme	plasma	1202:1207	arg1	proteins					1209:1216	plasma proteins	1202:1216	plasma proteins	1202:1216	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	3	39	theme	inhibitors	482:491	arg1	use					427:429	the use	423:429	the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations	423:561	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	9	40	theme	fungal	1581:1586	arg1	infections					1588:1597	fungal infections	1581:1597	fungal infections in humans	1581:1607	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	6	41	theme	liposomal	1018:1026	arg1	formulations					1028:1039	the liposomal formulations	1014:1039	the liposomal formulations	1014:1039	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	2	42	theme	efficient	259:267	arg1	earlier					205:211	earlier	205:211	earlier	205:211	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	2	42	theme	efficient	259:267	arg1	carrier					269:275	an efficient carrier	256:275	an efficient carrier of drugs and antigens	256:297	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	6	43	theme	PB/liposome-PB	840:853	arg1	systems					855:861	PB/liposome-PB systems	840:861	PB/liposome-PB systems	840:861	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	3	44	theme	cross	494:498	arg1	linkers					500:506	cross linkers	494:506	cross linkers	494:506	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	3	45	theme	dual	513:516	arg1	entrapment					518:527	dual entrapment	513:527	dual entrapment	513:527	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	3	46	theme	various	532:538	arg1	formulations					550:561	various liposomal formulations	532:561	various liposomal formulations	532:561	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	4	47	theme	different	589:597	arg1	formulations					599:610	the different formulations	585:610	the different formulations	585:610	In vitro efficacy of the different formulations was examined.
35164370	2	48	theme	plasma	228:233	arg1	PB					242:243	PB	242:243	PB	242:243	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	2	48	theme	plasma	228:233	arg1	beads					235:239	fibrin-based plasma beads	215:239	earlier a fibrin-based plasma beads (PB) system	205:251	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	8	49	with	docking	1377:1383	arg1	AmpB					1319:1322	AmpB	1319:1322	AmpB using isothermal titration calorimetry	1319:1361	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	5	50	theme	formulations	650:661	arg1	Pharmacokinetics					626:641	Pharmacokinetics	626:641	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound,	626:722	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	3	51	theme	liposomal	540:548	arg1	formulations					550:561	various liposomal formulations	532:561	various liposomal formulations	532:561	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	9	52	from	infections	1588:1597	arg1	humans					1602:1607	humans	1602:1607	humans	1602:1607	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	8	53	theme	slow	1427:1430	arg1	release					1432:1438	the slow release	1423:1438	the slow release of AmpB entrapped in PB alone	1423:1468	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	8	54	theme	Molecular	1219:1227	arg1	studies					1241:1247	Molecular interaction studies	1219:1247	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking	1219:1383	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	5	55	theme	Amphotericin	677:688	arg1	compound					714:721	an antifungal compound	700:721	an antifungal compound	700:721	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	5	55	theme	Amphotericin	677:688	arg1	AmpB					693:696	AmpB	693:696	AmpB	693:696	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	5	55	theme	Amphotericin	677:688	arg1	B					690:690	Amphotericin B	677:690	Amphotericin B (AmpB)	677:697	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	8	56	with	%	1302:1302	arg1	AmpB					1319:1322	AmpB	1319:1322	AmpB using isothermal titration calorimetry	1319:1361	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	7	57	theme	earlier	1149:1155	arg1	findings					1157:1164	our earlier findings	1145:1164	our earlier findings	1145:1164	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	1	58	theme	drug	136:139	arg1	delivery					150:157	drug and gene delivery	136:157	delivery	150:157	Fibrin-based systems offer promises in drug and gene delivery as well as tissue engineering.
35164370	6	59	theme	gradual	955:961	arg1	accumulation					963:974	its gradual accumulation	951:974	its gradual accumulation in the vital organs	951:994	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	8	60	theme	bovine	1252:1257	arg1	albumin					1265:1271	bovine serum albumin	1252:1271	bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking	1252:1383	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	5	61	theme	antifungal	703:712	arg1	compound					714:721	an antifungal compound	700:721	an antifungal compound	700:721	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	5	61	theme	antifungal	703:712	arg1	B					690:690	Amphotericin B	677:690	Amphotericin B (AmpB)	677:697	Pharmacokinetics of the formulations encapsulating Amphotericin B (AmpB), an antifungal compound, were investigated in Swiss albino mice.
35164370	0	62	theme	Combat	71:76	arg1	Infections					85:94	Combat Fungal Infections	71:94	Combat Fungal Infections	71:94	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	1	63	theme	gene	145:148	arg1	delivery					150:157	drug and gene delivery	136:157	delivery	150:157	Fibrin-based systems offer promises in drug and gene delivery as well as tissue engineering.
35164370	8	64	theme	albumin	1265:1271	arg1	studies					1241:1247	Molecular interaction studies	1219:1247	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking	1219:1383	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	7	65	theme	small	1126:1130	arg1	molecules					1132:1140	other small molecules	1120:1140	other small molecules in our earlier findings	1120:1164	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	7	66	from	PB	1093:1094	arg1	release					1072:1078	the slow release	1063:1078	the slow release of AmpB from PB	1063:1094	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	7	66	from	PB	1093:1094	arg1	unusual					1100:1106	unusual	1100:1106	unusual	1100:1106	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	6	67	from	accumulation	963:974	arg1	organs					989:994	the vital organs	979:994	the vital organs	979:994	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	6	67	from	accumulation	963:974	arg1	circulation					926:936	the circulation	922:936	the circulation	922:936	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	2	68	theme	antigens	290:297	arg1	earlier					205:211	earlier	205:211	earlier	205:211	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	2	68	theme	antigens	290:297	arg1	carrier					269:275	an efficient carrier	256:275	an efficient carrier of drugs and antigens	256:297	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	2	69	dep	earlier	205:211	arg1	system					246:251	a fibrin-based plasma beads (PB) system	213:251	earlier a fibrin-based plasma beads (PB) system	205:251	We established earlier a fibrin-based plasma beads (PB) system as an efficient carrier of drugs and antigens.
35164370	0	70	theme	Bead	7:10	arg1	Liposomes					22:30	Plasma Bead Entrapped Liposomes	0:30	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.	0:95	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	8	71	theme	plasma	1307:1312	arg1	%					1302:1302	approximately 60%	1286:1302	approximately 60% of plasma with AmpB using isothermal titration calorimetry	1286:1361	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	8	71	theme	plasma	1307:1312	arg1	docking					1377:1383	in silico docking	1367:1383	in silico docking	1367:1383	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	8	71	theme	plasma	1307:1312	arg1	plasma					1307:1312	plasma	1307:1312	plasma	1307:1312	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	3	72	theme	innovative	395:404	arg1	ideas					406:410	innovative ideas	395:410	innovative ideas	395:410	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	7	73	theme	AmpB	1083:1086	arg1	release					1072:1078	the slow release	1063:1078	the slow release of AmpB from PB	1063:1094	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	7	73	theme	AmpB	1083:1086	arg1	unusual					1100:1106	unusual	1100:1106	unusual	1100:1106	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
35164370	9	74	theme	safe	1535:1538	arg1	PB/liposome-PB					1503:1516	PB/liposome-PB	1503:1516	PB/liposome-PB	1503:1516	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	9	74	theme	safe	1535:1538	arg1	systems					1563:1569	safe and effective delivery systems	1535:1569	safe and effective delivery systems	1535:1569	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	6	75	theme	vital	983:987	arg1	organs					989:994	the vital organs	979:994	the vital organs	979:994	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	8	76	theme	isothermal	1330:1339	arg1	calorimetry					1351:1361	isothermal titration calorimetry	1330:1361	isothermal titration calorimetry	1330:1361	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	0	77	theme	Drug	47:50	arg1	System					61:66	a Potential Drug Delivery System	35:66	a Potential Drug Delivery System to Combat Fungal Infections	35:94	Plasma Bead Entrapped Liposomes as a Potential Drug Delivery System to Combat Fungal Infections.
35164370	6	78	theme	rapid	813:817	arg1	elimination					819:829	its rapid elimination	809:829	its rapid elimination (<72 h)	809:837	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	6	78	theme	rapid	813:817	arg1	h					836:836	<72 h	832:836	<72 h	832:836	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	9	79	theme	effective	1544:1552	arg1	PB/liposome-PB					1503:1516	PB/liposome-PB	1503:1516	PB/liposome-PB	1503:1516	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	9	79	theme	effective	1544:1552	arg1	systems					1563:1569	safe and effective delivery systems	1535:1569	safe and effective delivery systems	1535:1569	The above findings suggest that PB/liposome-PB could be used as safe and effective delivery systems to combat fungal infections in humans.
35164370	3	80	from	use	427:429	arg1	formulations					550:561	various liposomal formulations	532:561	various liposomal formulations	532:561	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	3	81	theme	matrices	460:467	arg1	use					427:429	the use	423:429	the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations	423:561	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	8	82	theme	in	1367:1368	arg1	docking					1377:1383	in silico docking	1367:1383	in silico docking	1367:1383	Molecular interaction studies of bovine serum albumin constituting approximately 60% of plasma with AmpB using isothermal titration calorimetry and in silico docking verify these interactions, explaining the slow release of AmpB entrapped in PB alone.
35164370	3	83	theme	alginate	441:448	arg1	matrices					460:467	plasma alginate composite matrices	434:467	plasma alginate composite matrices	434:467	In the present work, attempts were made to further improve its therapeutic efficacy exploiting innovative ideas, including the use of plasma alginate composite matrices, proteolytic inhibitors, cross linkers, and dual entrapment in various liposomal formulations.
35164370	6	84	theme	AmpB	797:800	arg1	administration					770:783	administration	770:783	administration of the free AmpB	770:800	While administration of the free AmpB led to its rapid elimination (<72 h), PB/liposome-PB systems were significantly effective in sustaining AmpB release in the circulation (>144 h) and its gradual accumulation in the vital organs, also compared to the liposomal formulations alone.
35164370	7	85	theme	strong	1178:1183	arg1	interaction					1185:1195	strong interaction	1178:1195	strong interaction with plasma proteins	1178:1216	Interestingly, the slow release of AmpB from PB was unusual compared to other small molecules in our earlier findings, suggesting strong interaction with plasma proteins.
37273228	4	0	theme	Lipid	811:815	arg1	metabolites					817:827	Lipid metabolites	811:827	Lipid metabolites such as choline and hippuric acid	811:861	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	4	0	theme	Lipid	811:815	arg1	choline					837:843	choline	837:843	choline	837:843	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	4	0	theme	Lipid	811:815	arg1	acid					858:861	hippuric acid	849:861	hippuric acid	849:861	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	15	1	from	diet	2935:2938	arg1	pandas					2959:2964	young and adult pandas	2943:2964	young and adult pandas	2943:2964	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	4	2	theme	Adult	975:979	arg1	groups					981:986	the Young and Adult groups	961:986	the Young and Adult groups	961:986	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	11	3	from	data	2098:2101	arg1	results					2048:2054	Our results	2044:2054	Our results from metabolome, 16S rRNA, and metagenome data	2044:2101	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	8	4	theme	age	1778:1780	arg1	groups					1782:1787	age groups	1778:1787	age groups	1778:1787	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	1	5	theme	gut	387:389	arg1	microbiota					391:400	gut microbiota	387:400	gut microbiota	387:400	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	16	6	contain	has	3065:3067	arg1	axis					3060:3063	the gut microbiota-bile acid axis	3031:3063	the gut microbiota-bile acid axis	3031:3063	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	16	6	contain	has	3065:3067	arg2	role					3082:3085	an important role	3069:3085	an important role	3069:3085	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	15	7	theme	gut	2827:2829	arg1	microbiota					2831:2840	gut microbiota	2827:2840	gut microbiota	2827:2840	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	9	8	theme	antibiotic	1813:1822	arg1	ARGs					1842:1845	ARGs	1842:1845	ARGs	1842:1845	We also identified 237 antibiotic resistance genes (ARGs) whose number and diversity increased with age.
37273228	9	8	theme	antibiotic	1813:1822	arg1	genes					1835:1839	237 antibiotic resistance genes	1809:1839	237 antibiotic resistance genes (ARGs) whose number and diversity increased with age	1809:1892	We also identified 237 antibiotic resistance genes (ARGs) whose number and diversity increased with age.
37273228	16	9	theme	pandas	3203:3208	arg1	metabolism					3183:3192	the lipid metabolism	3173:3192	the lipid metabolism of giant pandas	3173:3208	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	16	10	theme	16S	2983:2985	arg1	rRNA					2987:2990	16S rRNA	2983:2990	16S rRNA	2983:2990	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	13	11	from	changes	2521:2527	arg1	metabolites					2532:2542	metabolites	2532:2542	metabolites	2532:2542	It is therefore crucial to investigate the dynamic changes in metabolites as giant pandas grow and physiologically adapt to their herbivorous diet.
37273228	2	12	theme	age	587:589	arg1	groups					591:596	the age groups	583:596	the age groups	583:596	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	11	13	theme	giant	2267:2271	arg1	panda					2300:2304	giant pandas.IMPORTANCEThe giant panda	2267:2304	giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous	2267:2367	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	3	14	theme	gut	665:667	arg1	microbiota					669:678	the gut microbiota	661:678	the gut microbiota	661:678	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	5	15	from	decrease	1104:1111	arg1	pandas					1167:1172	adult and old pandas	1153:1172	adult and old pandas	1153:1172	However, there was a decrease in the α-diversity of gut microbiota in adult and old pandas, who exclusively consume bamboo.
37273228	5	15	from	decrease	1104:1111	arg1	α-diversity					1120:1130	the α-diversity	1116:1130	the α-diversity of gut microbiota	1116:1148	However, there was a decrease in the α-diversity of gut microbiota in adult and old pandas, who exclusively consume bamboo.
37273228	1	16	theme	pandas	411:416	arg1	metabolism					372:381	the metabolism	368:381	the metabolism	368:381	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	16	theme	pandas	411:416	arg1	microbiota					391:400	gut microbiota	387:400	gut microbiota	387:400	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	6	17	theme	bacteria	1425:1432	arg1	abundance					1401:1409	the abundance	1397:1409	the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia	1397:1479	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	15	18	dep	transition	2869:2878	arg1	response					2853:2860	response	2853:2860	response	2853:2860	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	10	19	theme	acids	1974:1978	arg1	abundance					1956:1964	the abundance	1952:1964	the abundance of bile acids	1952:1978	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	10	19	theme	acids	1974:1978	arg1	Bifidobacterium					2027:2041	Bifidobacterium	2027:2041	Bifidobacterium	2027:2041	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	10	19	theme	acids	1974:1978	arg1	bacteria					1988:1995	gut bacteria	1984:1995	gut bacteria	1984:1995	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	10	19	theme	acids	1974:1978	arg1	Lactobacillus					2009:2021	Lactobacillus	2009:2021	Lactobacillus	2009:2021	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	14	20	theme	age	2757:2759	arg1	groups					2761:2766	four age groups	2752:2766	four age groups	2752:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	12	21	theme	related	2409:2415	arg1	mechanisms					2427:2436	The giant panda's specialized diet and related metabolic mechanisms	2370:2436	mechanisms	2427:2436	The giant panda's specialized diet and related metabolic mechanisms have not been fully understood.
37273228	16	22	theme	metagenome	2997:3006	arg1	results					3008:3014	The metabolome, 16S rRNA, and metagenome results	2967:3014	The metabolome, 16S rRNA, and metagenome results	2967:3014	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	4	23	located	found	1059:1063	arg1	group					1076:1080	the Old group	1068:1080	the Old group	1068:1080	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	4	23	located	found	1059:1063	arg2	stress					1005:1010	oxidative stress	995:1010	oxidative stress	995:1010	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	4	23	located	found	1059:1063	arg2	metabolites					1037:1047	inflammatory related metabolites	1016:1047	inflammatory related metabolites	1016:1047	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	4	24	theme	related	1029:1035	arg1	metabolites					1037:1047	inflammatory related metabolites	1016:1047	inflammatory related metabolites	1016:1047	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	11	25	from	metabolome	2061:2070	arg1	results					2048:2054	Our results	2044:2054	Our results from metabolome, 16S rRNA, and metagenome data	2044:2101	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	7	26	theme	pathogenic	1533:1542	arg1	bacteria					1544:1551	several potential pathogenic bacteria	1515:1551	several potential pathogenic bacteria	1515:1551	Notably, several potential pathogenic bacteria had relatively high abundances, especially in the Young group.
37273228	2	27	theme	identified	491:500	arg1	metabolites					502:512	1,376 identified metabolites	485:512	1,376 identified metabolites	485:512	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	0	28	from	transition	81:90	arg1	pandas					101:106	giant pandas	95:106	giant pandas	95:106	Age-related alterations in metabolome and microbiome provide insights in dietary transition in giant pandas.
37273228	14	29	theme	giant	2734:2738	arg1	pandas					2740:2745	captive giant pandas	2726:2745	captive giant pandas from four age groups	2726:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	11	30	theme	metabolism	2202:2211	arg1	regulation					2176:2185	the regulation	2172:2185	the regulation of age-related metabolism	2172:2211	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	3	31	theme	milk-dominant	725:737	arg1	diet					739:742	a milk-dominant diet	723:742	a milk-dominant diet	723:742	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	6	32	theme	bacteria	1224:1231	arg1	abundance					1211:1219	The abundance	1207:1219	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium,	1207:1332	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	1	33	theme	age-related	345:355	arg1	changes					357:363	age-related changes	345:363	age-related changes in the metabolism and gut microbiota of giant pandas	345:416	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	8	34	theme	cellulose	1675:1683	arg1	genes					1695:1699	cellulose degrading genes	1675:1699	cellulose degrading genes	1675:1699	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	7	35	theme	several	1515:1521	arg1	bacteria					1544:1551	several potential pathogenic bacteria	1515:1551	several potential pathogenic bacteria	1515:1551	Notably, several potential pathogenic bacteria had relatively high abundances, especially in the Young group.
37273228	2	36	theme	differential	538:549	arg1	SDMs					564:567	SDMs	564:567	SDMs	564:567	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	2	36	theme	differential	538:549	arg1	metabolites					551:561	152 significantly differential metabolites	520:561	152 significantly differential metabolites (SDMs) found across the age groups	520:596	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	1	37	theme	UPLC-MS-based	122:134	arg1	metabolomics					136:147	UPLC-MS-based metabolomics	122:147	UPLC-MS-based metabolomics	122:147	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	3	38	theme	adult	797:801	arg1	pandas					803:808	young and adult pandas	787:808	young and adult pandas	787:808	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	4	39	theme	hippuric	849:856	arg1	acid					858:861	hippuric acid	849:861	hippuric acid	849:861	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	14	40	theme	fecal	2709:2713	arg1	samples					2715:2721	the fecal samples	2705:2721	the fecal samples of captive giant pandas from four age groups	2705:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	11	41	theme	Carnivora	2331:2339	arg1	member					2311:2316	a member	2309:2316	a member of the order Carnivora	2309:2339	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	16	42	theme	new	3155:3157	arg1	insights					3159:3166	new insights	3155:3166	new insights into the lipid metabolism of giant pandas	3155:3208	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	3	43	theme	young	787:791	arg1	pandas					803:808	young and adult pandas	787:808	young and adult pandas	787:808	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	4	44	theme	many	899:902	arg1	metabolites					920:930	many plant secondary metabolites	899:930	many plant secondary metabolites	899:930	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	10	45	theme	significant	1911:1921	arg1	correlation					1932:1942	a significant positive correlation	1909:1942	a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium	1909:2041	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	2	46	theme	metabolite	440:449	arg1	profiles					451:458	the metabolite profiles	436:458	the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups	436:596	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	5	47	theme	old	1163:1165	arg1	pandas					1167:1172	adult and old pandas	1153:1172	adult and old pandas	1153:1172	However, there was a decrease in the α-diversity of gut microbiota in adult and old pandas, who exclusively consume bamboo.
37273228	8	48	theme	CAZymes	1719:1725	arg1	seven					1706:1710	seven	1706:1710	seven	1706:1710	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	8	48	theme	CAZymes	1719:1725	arg1	CAZymes					1719:1725	the CAZymes	1715:1725	the CAZymes	1715:1725	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	4	49	theme	Cub	884:886	arg1	group					888:892	the Cub group	880:892	the Cub group	880:892	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	6	50	theme	cellulose-rich	1261:1274	arg1	food					1276:1279	cellulose-rich food	1261:1279	cellulose-rich food	1261:1279	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	2	51	theme	pandas	469:474	arg1	profiles					451:458	the metabolite profiles	436:458	the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups	436:596	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	16	52	theme	giant	3197:3201	arg1	pandas					3203:3208	giant pandas	3197:3208	giant pandas	3197:3208	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	11	53	theme	giant	2294:2298	arg1	panda					2300:2304	giant pandas.IMPORTANCEThe giant panda	2267:2304	giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous	2267:2367	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	13	54	theme	giant	2547:2551	arg1	pandas					2553:2558	giant pandas	2547:2558	giant pandas	2547:2558	It is therefore crucial to investigate the dynamic changes in metabolites as giant pandas grow and physiologically adapt to their herbivorous diet.
37273228	1	55	from	metabolomics	136:147	arg1	samples					199:205	the fecal samples	189:205	the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca)	189:257	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	16	56	theme	microbiota-bile	3039:3053	arg1	axis					3060:3063	the gut microbiota-bile acid axis	3031:3063	the gut microbiota-bile acid axis	3031:3063	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	11	57	from	rRNA	2077:2080	arg1	results					2048:2054	Our results	2044:2054	Our results from metabolome, 16S rRNA, and metagenome data	2044:2101	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	0	58	theme	Age-related	0:10	arg1	alterations					12:22	Age-related alterations	0:22	Age-related alterations in metabolome and microbiome	0:51	Age-related alterations in metabolome and microbiome provide insights in dietary transition in giant pandas.
37273228	16	59	theme	metabolism	3120:3129	arg1	regulation					3094:3103	the regulation	3090:3103	the regulation of age-related metabolism	3090:3129	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	11	60	theme	important	2117:2125	arg1	role					2127:2130	the important role	2113:2130	the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism	2113:2211	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	11	61	theme	16S	2073:2075	arg1	rRNA					2077:2080	16S rRNA	2073:2080	16S rRNA	2073:2080	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	14	62	theme	metabolomics	2653:2664	arg1	rRNA					2670:2673	UPLC-MS-based metabolomics 16S rRNA	2639:2673	UPLC-MS-based metabolomics 16S rRNA	2639:2673	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	3	63	from	diet	779:782	arg1	pandas					803:808	young and adult pandas	787:808	young and adult pandas	787:808	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	15	64	theme	milk-dominant	2887:2899	arg1	diet					2901:2904	a milk-dominant diet	2885:2904	a milk-dominant diet in cubs	2885:2912	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	11	65	theme	metagenome	2087:2096	arg1	data					2098:2101	metagenome data	2087:2101	metagenome data	2087:2101	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	14	66	from	samples	2715:2721	arg1	groups					2761:2766	four age groups	2752:2766	four age groups	2752:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	16	67	theme	lipid	3177:3181	arg1	metabolism					3183:3192	the lipid metabolism	3173:3192	the lipid metabolism of giant pandas	3173:3208	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	10	68	theme	gut	1984:1986	arg1	Bifidobacterium					2027:2041	Bifidobacterium	2027:2041	Bifidobacterium	2027:2041	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	10	68	theme	gut	1984:1986	arg1	bacteria					1988:1995	gut bacteria	1984:1995	gut bacteria	1984:1995	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	10	68	theme	gut	1984:1986	arg1	Lactobacillus					2009:2021	Lactobacillus	2009:2021	Lactobacillus	2009:2021	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	11	69	from	role	2127:2130	arg1	regulation					2176:2185	the regulation	2172:2185	the regulation of age-related metabolism	2172:2211	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	1	70	theme	captive	213:219	arg1	pandas					227:232	44 captive giant pandas	210:232	44 captive giant pandas (Ailuropoda melanoleuca)	210:257	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	70	theme	captive	213:219	arg1	melanoleuca					246:256	melanoleuca	246:256	melanoleuca	246:256	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	16	71	theme	gut	3035:3037	arg1	axis					3060:3063	the gut microbiota-bile acid axis	3031:3063	the gut microbiota-bile acid axis	3031:3063	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	4	72	theme	inflammatory	1016:1027	arg1	metabolites					1037:1047	inflammatory related metabolites	1016:1047	inflammatory related metabolites	1016:1047	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	12	73	theme	metabolic	2417:2425	arg1	mechanisms					2427:2436	The giant panda's specialized diet and related metabolic mechanisms	2370:2436	mechanisms	2427:2436	The giant panda's specialized diet and related metabolic mechanisms have not been fully understood.
37273228	2	74	with	metabolites	502:512	arg1	SDMs					564:567	SDMs	564:567	SDMs	564:567	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	2	74	with	metabolites	502:512	arg1	metabolites					551:561	152 significantly differential metabolites	520:561	152 significantly differential metabolites (SDMs) found across the age groups	520:596	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	1	75	theme	pandas	227:232	arg1	samples					199:205	the fecal samples	189:205	the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca)	189:257	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	3	76	theme	panda	747:751	arg1	cubs					753:756	panda cubs	747:756	panda cubs	747:756	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	15	77	theme	adult	2953:2957	arg1	pandas					2959:2964	young and adult pandas	2943:2964	young and adult pandas	2943:2964	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	4	78	theme	secondary	910:918	arg1	metabolites					920:930	many plant secondary metabolites	899:930	many plant secondary metabolites	899:930	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	16	79	theme	acid	3055:3058	arg1	axis					3060:3063	the gut microbiota-bile acid axis	3031:3063	the gut microbiota-bile acid axis	3031:3063	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	8	80	contain	had	1727:1729	arg2	abundances					1731:1740	abundances	1731:1740	abundances that significantly differed between age groups	1731:1787	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	8	80	contain	had	1727:1729	arg1	seven					1706:1710	seven	1706:1710	seven	1706:1710	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	8	80	contain	had	1727:1729	arg1	CAZymes					1719:1725	the CAZymes	1715:1725	the CAZymes	1715:1725	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	15	81	theme	young	2943:2947	arg1	pandas					2959:2964	young and adult pandas	2943:2964	young and adult pandas	2943:2964	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	11	82	theme	lipid	2247:2251	arg1	metabolism					2253:2262	the lipid metabolism	2243:2262	the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous	2243:2367	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	14	83	from	groups	2761:2766	arg1	pandas					2740:2745	captive giant pandas	2726:2745	captive giant pandas from four age groups	2726:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	14	83	from	groups	2761:2766	arg1	samples					2715:2721	the fecal samples	2705:2721	the fecal samples of captive giant pandas from four age groups	2705:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	15	84	theme	microbiota	2831:2840	arg1	metabolites					2783:2793	metabolites	2783:2793	metabolites	2783:2793	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	15	84	theme	microbiota	2831:2840	arg1	composition/function					2803:2822	the composition/function	2799:2822	the composition/function of gut microbiota	2799:2840	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	9	85	theme	resistance	1824:1833	arg1	ARGs					1842:1845	ARGs	1842:1845	ARGs	1842:1845	We also identified 237 antibiotic resistance genes (ARGs) whose number and diversity increased with age.
37273228	9	85	theme	resistance	1824:1833	arg1	genes					1835:1839	237 antibiotic resistance genes	1809:1839	237 antibiotic resistance genes (ARGs) whose number and diversity increased with age	1809:1892	We also identified 237 antibiotic resistance genes (ARGs) whose number and diversity increased with age.
37273228	8	86	theme	Metagenomic	1616:1626	arg1	analysis					1628:1635	Metagenomic analysis	1616:1635	Metagenomic analysis	1616:1635	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	11	87	theme	new	2225:2227	arg1	insights					2229:2236	new insights	2225:2236	new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous	2225:2367	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	1	88	from	groups	273:278	arg1	metabolomics					136:147	UPLC-MS-based metabolomics	122:147	UPLC-MS-based metabolomics	122:147	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	88	from	groups	273:278	arg1	rRNA					154:157	16S rRNA	150:157	16S rRNA	150:157	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	88	from	groups	273:278	arg1	sequencing					175:184	metagenome sequencing	164:184	metagenome sequencing	164:184	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	89	theme	giant	405:409	arg1	pandas					411:416	giant pandas	405:416	giant pandas	405:416	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	90	theme	metagenome	164:173	arg1	sequencing					175:184	metagenome sequencing	164:184	metagenome sequencing	164:184	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	4	91	theme	Old	1072:1074	arg1	group					1076:1080	the Old group	1068:1080	the Old group	1068:1080	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	3	92	theme	microbiota	669:678	arg1	metabolites					617:627	the metabolites	613:627	the metabolites	613:627	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	3	92	theme	microbiota	669:678	arg1	composition/function					637:656	the composition/function	633:656	the composition/function of the gut microbiota	633:678	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	6	93	theme	beneficial	1414:1423	arg1	Sarcina					1460:1466	Sarcina	1460:1466	Sarcina	1460:1466	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	6	93	theme	beneficial	1414:1423	arg1	bacteria					1425:1432	beneficial bacteria	1414:1432	beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia	1414:1479	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	6	93	theme	beneficial	1414:1423	arg1	Faecalibacterium					1442:1457	Faecalibacterium	1442:1457	Faecalibacterium	1442:1457	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	6	93	theme	beneficial	1414:1423	arg1	Blautia					1473:1479	Blautia	1473:1479	Blautia	1473:1479	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	1	94	from	changes	357:363	arg1	metabolism					372:381	the metabolism	368:381	the metabolism	368:381	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	94	from	changes	357:363	arg1	microbiota					391:400	gut microbiota	387:400	gut microbiota	387:400	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	95	theme	fecal	193:197	arg1	samples					199:205	the fecal samples	189:205	the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca)	189:257	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	7	96	theme	potential	1523:1531	arg1	bacteria					1544:1551	several potential pathogenic bacteria	1515:1551	several potential pathogenic bacteria	1515:1551	Notably, several potential pathogenic bacteria had relatively high abundances, especially in the Young group.
37273228	14	97	theme	pandas	2740:2745	arg1	samples					2715:2721	the fecal samples	2705:2721	the fecal samples of captive giant pandas from four age groups	2705:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	11	98	theme	age-related	2190:2200	arg1	metabolism					2202:2211	age-related metabolism	2190:2211	age-related metabolism	2190:2211	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	6	99	theme	related	1233:1239	arg1	Clostridium					1321:1331	Clostridium	1321:1331	Clostridium	1321:1331	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	6	99	theme	related	1233:1239	arg1	Streptococcus					1302:1314	Streptococcus	1302:1314	Streptococcus	1302:1314	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	6	99	theme	related	1233:1239	arg1	bacteria					1224:1231	bacteria	1224:1231	bacteria related to the digestion of cellulose-rich food	1224:1279	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	6	99	theme	related	1233:1239	arg1	Firmicutes					1290:1299	Firmicutes	1290:1299	Firmicutes	1290:1299	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	8	100	theme	CAZyme	1652:1657	arg1	genes					1695:1699	cellulose degrading genes	1675:1699	cellulose degrading genes	1675:1699	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	8	100	theme	CAZyme	1652:1657	arg1	genes					1659:1663	277 CAZyme genes	1648:1663	277 CAZyme genes including cellulose degrading genes	1648:1699	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	14	101	theme	captive	2726:2732	arg1	pandas					2740:2745	captive giant pandas	2726:2745	captive giant pandas from four age groups	2726:2766	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	11	102	theme	acid	2159:2162	arg1	axis					2164:2167	the gut microbiota-bile acid axis	2135:2167	the gut microbiota-bile acid axis	2135:2167	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	10	103	dep	significant	1911:1921	arg1	positive					1923:1930	positive	1923:1930	positive	1923:1930	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	11	104	theme	order	2325:2329	arg1	Carnivora					2331:2339	the order Carnivora	2321:2339	the order Carnivora	2321:2339	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	8	105	theme	degrading	1685:1693	arg1	genes					1695:1699	cellulose degrading genes	1675:1699	cellulose degrading genes	1675:1699	Metagenomic analysis identified 277 CAZyme genes including cellulose degrading genes, and seven of the CAZymes had abundances that significantly differed between age groups.
37273228	12	106	theme	giant	2374:2378	arg1	panda					2380:2384	The giant panda	2370:2384	The giant panda	2370:2384	The giant panda's specialized diet and related metabolic mechanisms have not been fully understood.
37273228	11	107	dep	panda	2300:2304	arg1	herbivorous					2357:2367	herbivorous	2357:2367	herbivorous	2357:2367	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	11	107	dep	panda	2300:2304	arg1	member					2311:2316	a member	2309:2316	a member of the order Carnivora	2309:2339	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	0	108	theme	dietary	73:79	arg1	transition					81:90	dietary transition	73:90	dietary transition in giant pandas	73:106	Age-related alterations in metabolome and microbiome provide insights in dietary transition in giant pandas.
37273228	1	109	theme	16S	150:152	arg1	rRNA					154:157	16S rRNA	150:157	16S rRNA	150:157	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	110	dep	groups	273:278	arg1	Adult					299:303	Adult	299:303	Adult	299:303	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	110	dep	groups	273:278	arg1	Old					310:312	Old	310:312	Old	310:312	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	110	dep	groups	273:278	arg1	Young					292:296	Young	292:296	Young	292:296	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	5	111	theme	gut	1135:1137	arg1	microbiota					1139:1148	gut microbiota	1135:1148	gut microbiota	1135:1148	However, there was a decrease in the α-diversity of gut microbiota in adult and old pandas, who exclusively consume bamboo.
37273228	0	112	theme	giant	95:99	arg1	pandas					101:106	giant pandas	95:106	giant pandas	95:106	Age-related alterations in metabolome and microbiome provide insights in dietary transition in giant pandas.
37273228	1	113	from	sequencing	175:184	arg1	samples					199:205	the fecal samples	189:205	the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca)	189:257	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	0	114	from	insights	61:68	arg1	transition					81:90	dietary transition	73:90	dietary transition in giant pandas	73:106	Age-related alterations in metabolome and microbiome provide insights in dietary transition in giant pandas.
37273228	4	115	theme	plant	904:908	arg1	metabolites					920:930	many plant secondary metabolites	899:930	many plant secondary metabolites	899:930	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	0	116	from	alterations	12:22	arg1	microbiome					42:51	microbiome	42:51	microbiome	42:51	Age-related alterations in metabolome and microbiome provide insights in dietary transition in giant pandas.
37273228	0	116	from	alterations	12:22	arg1	metabolome					27:36	metabolome	27:36	metabolome	27:36	Age-related alterations in metabolome and microbiome provide insights in dietary transition in giant pandas.
37273228	11	117	theme	panda	2300:2304	arg1	metabolism					2253:2262	the lipid metabolism	2243:2262	the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous	2243:2367	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	6	118	theme	food	1276:1279	arg1	digestion					1248:1256	the digestion	1244:1256	the digestion of cellulose-rich food	1244:1279	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	3	119	dep	transition	707:716	arg1	response					691:698	response	691:698	response	691:698	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	16	120	theme	important	3072:3080	arg1	role					3082:3085	an important role	3069:3085	an important role	3069:3085	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	11	121	theme	axis	2164:2167	arg1	role					2127:2130	the important role	2113:2130	the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism	2113:2211	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	1	122	dep	Cub	287:289	arg1	i.e.					281:284	i.e.	281:284	i.e.	281:284	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	3	123	from	diet	739:742	arg1	transition					707:716	the transition	703:716	the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas	703:808	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	11	124	theme	pandas.IMPORTANCEThe	2273:2292	arg1	panda					2300:2304	giant pandas.IMPORTANCEThe giant panda	2267:2304	giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous	2267:2367	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	12	125	theme	specialized	2388:2398	arg1	diet					2400:2403	The giant panda's specialized diet and related metabolic mechanisms	2370:2436	diet	2400:2403	The giant panda's specialized diet and related metabolic mechanisms have not been fully understood.
37273228	3	126	theme	bamboo-specific	763:777	arg1	diet					779:782	a bamboo-specific diet	761:782	a bamboo-specific diet in young and adult pandas	761:808	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	16	127	theme	metabolome	2971:2980	arg1	results					3008:3014	The metabolome, 16S rRNA, and metagenome results	2967:3014	The metabolome, 16S rRNA, and metagenome results	2967:3014	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	1	128	from	rRNA	154:157	arg1	samples					199:205	the fecal samples	189:205	the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca)	189:257	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	7	129	theme	Young	1603:1607	arg1	group					1609:1613	the Young group	1599:1613	the Young group	1599:1613	Notably, several potential pathogenic bacteria had relatively high abundances, especially in the Young group.
37273228	14	130	theme	metagenome	2680:2689	arg1	sequencing					2691:2700	metagenome sequencing	2680:2700	metagenome sequencing	2680:2700	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	2	131	theme	giant	463:467	arg1	pandas					469:474	giant pandas	463:474	giant pandas	463:474	We characterized the metabolite profiles of giant pandas based on 1,376 identified metabolites, with 152 significantly differential metabolites (SDMs) found across the age groups.
37273228	1	132	theme	giant	221:225	arg1	pandas					227:232	44 captive giant pandas	210:232	44 captive giant pandas (Ailuropoda melanoleuca)	210:257	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	1	132	theme	giant	221:225	arg1	melanoleuca					246:256	melanoleuca	246:256	melanoleuca	246:256	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	11	133	theme	gut	2139:2141	arg1	axis					2164:2167	the gut microbiota-bile acid axis	2135:2167	the gut microbiota-bile acid axis	2135:2167	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	3	134	from	transition	707:716	arg1	cubs					753:756	panda cubs	747:756	panda cubs	747:756	We found that the metabolites and the composition/function of the gut microbiota changed in response to the transition from a milk-dominant diet in panda cubs to a bamboo-specific diet in young and adult pandas.
37273228	13	135	theme	dynamic	2513:2519	arg1	changes					2521:2527	the dynamic changes	2509:2527	the dynamic changes in metabolites	2509:2542	It is therefore crucial to investigate the dynamic changes in metabolites as giant pandas grow and physiologically adapt to their herbivorous diet.
37273228	1	136	theme	age	269:271	arg1	groups					273:278	four age groups	264:278	four age groups (i.e., Cub, Young, Adult, and Old)	264:313	We conducted UPLC-MS-based metabolomics, 16S rRNA, and metagenome sequencing on the fecal samples of 44 captive giant pandas (Ailuropoda melanoleuca) from four age groups (i.e., Cub, Young, Adult, and Old) to comprehensively understand age-related changes in the metabolism and gut microbiota of giant pandas.
37273228	16	137	theme	age-related	3108:3118	arg1	metabolism					3120:3129	age-related metabolism	3108:3129	age-related metabolism	3108:3129	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	7	138	contain	had	1553:1555	arg1	bacteria					1544:1551	several potential pathogenic bacteria	1515:1551	several potential pathogenic bacteria	1515:1551	Notably, several potential pathogenic bacteria had relatively high abundances, especially in the Young group.
37273228	7	138	contain	had	1553:1555	arg2	abundances					1573:1582	relatively high abundances	1557:1582	relatively high abundances	1557:1582	Notably, several potential pathogenic bacteria had relatively high abundances, especially in the Young group.
37273228	14	139	theme	16S	2666:2668	arg1	rRNA					2670:2673	UPLC-MS-based metabolomics 16S rRNA	2639:2673	UPLC-MS-based metabolomics 16S rRNA	2639:2673	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	4	140	theme	Young	965:969	arg1	groups					981:986	the Young and Adult groups	961:986	the Young and Adult groups	961:986	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	16	141	theme	rRNA	2987:2990	arg1	results					3008:3014	The metabolome, 16S rRNA, and metagenome results	2967:3014	The metabolome, 16S rRNA, and metagenome results	2967:3014	The metabolome, 16S rRNA, and metagenome results highlight that the gut microbiota-bile acid axis has an important role in the regulation of age-related metabolism, and our study provides new insights into the lipid metabolism of giant pandas.
37273228	13	142	theme	herbivorous	2600:2610	arg1	diet					2612:2615	their herbivorous diet	2594:2615	their herbivorous diet	2594:2615	It is therefore crucial to investigate the dynamic changes in metabolites as giant pandas grow and physiologically adapt to their herbivorous diet.
37273228	7	143	theme	high	1568:1571	arg1	abundances					1573:1582	relatively high abundances	1557:1582	relatively high abundances	1557:1582	Notably, several potential pathogenic bacteria had relatively high abundances, especially in the Young group.
37273228	14	144	theme	UPLC-MS-based	2639:2651	arg1	rRNA					2670:2673	UPLC-MS-based metabolomics 16S rRNA	2639:2673	UPLC-MS-based metabolomics 16S rRNA	2639:2673	This study conducted UPLC-MS-based metabolomics 16S rRNA, and metagenome sequencing on the fecal samples of captive giant pandas from four age groups.
37273228	15	145	theme	bamboo-specific	2919:2933	arg1	diet					2935:2938	a bamboo-specific diet	2917:2938	a bamboo-specific diet in young and adult pandas	2917:2964	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	11	146	theme	microbiota-bile	2143:2157	arg1	axis					2164:2167	the gut microbiota-bile acid axis	2135:2167	the gut microbiota-bile acid axis	2135:2167	Our results from metabolome, 16S rRNA, and metagenome data highlight the important role of the gut microbiota-bile acid axis in the regulation of age-related metabolism and provide new insights into the lipid metabolism of giant pandas.IMPORTANCEThe giant panda is a member of the order Carnivora but is entirely herbivorous.
37273228	15	147	from	diet	2901:2904	arg1	transition					2869:2878	the transition	2865:2878	the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas	2865:2964	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	15	147	from	diet	2901:2904	arg1	cubs					2909:2912	cubs	2909:2912	cubs	2909:2912	We found that metabolites and the composition/function of gut microbiota changed in response to the transition from a milk-dominant diet in cubs to a bamboo-specific diet in young and adult pandas.
37273228	4	148	theme	oxidative	995:1003	arg1	stress					1005:1010	oxidative stress	995:1010	oxidative stress	995:1010	Lipid metabolites such as choline and hippuric acid were enriched in the Cub group, and many plant secondary metabolites were significantly higher in the Young and Adult groups, while oxidative stress and inflammatory related metabolites were only found in the Old group.
37273228	6	149	theme	Adult	1378:1382	arg1	group					1384:1388	the Adult group	1374:1388	the Adult group	1374:1388	The abundance of bacteria related to the digestion of cellulose-rich food, such as Firmicutes, Streptococcus, and Clostridium, significantly increased from the Cub to the Adult group, while the abundance of beneficial bacteria such as Faecalibacterium, Sarcina, and Blautia significantly decreased.
37273228	5	150	theme	microbiota	1139:1148	arg1	α-diversity					1120:1130	the α-diversity	1116:1130	the α-diversity of gut microbiota	1116:1148	However, there was a decrease in the α-diversity of gut microbiota in adult and old pandas, who exclusively consume bamboo.
37273228	10	151	theme	bile	1969:1972	arg1	acids					1974:1978	bile acids	1969:1978	bile acids	1969:1978	We also found a significant positive correlation between the abundance of bile acids and gut bacteria, especially Lactobacillus and Bifidobacterium.
37273228	5	152	theme	adult	1153:1157	arg1	pandas					1167:1172	adult and old pandas	1153:1172	adult and old pandas	1153:1172	However, there was a decrease in the α-diversity of gut microbiota in adult and old pandas, who exclusively consume bamboo.
37129938	9	0	theme	fatty	1168:1172	arg1	acids					1174:1178	The major fatty acids	1158:1178	The major fatty acids of strain FJAT-49754T	1158:1200	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	9	0	theme	fatty	1168:1172	arg1	anteiso-C15 					1207:1218	anteiso-C15 	1207:1218	anteiso-C15 	1207:1218	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	3	1	theme	rRNA	231:234	arg1	similarity					250:259	the highest 16S rRNA gene sequence similarity	215:259	the highest 16S rRNA gene sequence similarity	215:259	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	7	2	theme	NaCl	941:944	arg1	tolerance					946:954	NaCl tolerance	941:954	NaCl tolerance	941:954	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	3	theme	FJAT-49754T	848:858	arg1	Growth					831:836	Growth	831:836	Growth of strain FJAT-49754T	831:858	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	11	4	theme	citrea	1443:1448	arg1	sp					1450:1451	the name Lederbergia citrea sp	1422:1451	the name Lederbergia citrea sp	1422:1451	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	3	5	theme	gene	236:239	arg1	similarity					250:259	the highest 16S rRNA gene sequence similarity	215:259	the highest 16S rRNA gene sequence similarity	215:259	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	11	6	theme	novel	1372:1376	arg1	species					1378:1384	a novel species	1370:1384	a novel species	1370:1384	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	7	7	from	pH	904:905	arg1	optimum					886:892	optimum	886:892	optimum	886:892	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	10	8	theme	FJAT-49754T	1288:1298	arg1	content					1270:1276	The genomic DNA G+C content	1250:1276	The genomic DNA G+C content of strain FJAT-49754T	1250:1298	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	10	8	theme	FJAT-49754T	1288:1298	arg1	%					1309:1309	38.7 %	1304:1309	38.7 %	1304:1309	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	5	9	theme	species	680:686	arg1	delineation					688:698	prokaryotic species delineation	668:698	prokaryotic species delineation	668:698	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	11	10	theme	Lederbergia	1399:1409	arg1	species					1378:1384	a novel species	1370:1384	a novel species	1370:1384	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	3	11	theme	highest	219:225	arg1	similarity					250:259	the highest 16S rRNA gene sequence similarity	215:259	the highest 16S rRNA gene sequence similarity	215:259	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	1	12	theme	citrus	43:48	arg1	rhizosphere					50:60	citrus rhizosphere	43:60	citrus rhizosphere	43:60	nov., isolated from citrus rhizosphere.
37129938	11	13	theme	Lederbergia	1431:1441	arg1	sp					1450:1451	the name Lederbergia citrea sp	1422:1451	the name Lederbergia citrea sp	1422:1451	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	8	14	theme	only	1003:1006	arg1	menaquinone					1008:1018	the only menaquinone	999:1018	the only menaquinone detected in strain FJAT-49754T	999:1049	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	8	14	theme	only	1003:1006	arg1	MK-7					990:993	MK-7	990:993	MK-7	990:993	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	2	15	theme	citrus	162:167	arg1	sample					186:191	the citrus rhizosphere soil sample	158:191	the citrus rhizosphere soil sample	158:191	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	3	16	theme	16S	227:229	arg1	similarity					250:259	the highest 16S rRNA gene sequence similarity	215:259	the highest 16S rRNA gene sequence similarity	215:259	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	11	17	theme	genus	1393:1397	arg1	Lederbergia					1399:1409	the genus Lederbergia	1389:1409	the genus Lederbergia	1389:1409	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	9	18	theme	FJAT-49754T	1190:1200	arg1	acids					1174:1178	The major fatty acids	1158:1178	The major fatty acids of strain FJAT-49754T	1158:1200	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	9	18	theme	FJAT-49754T	1190:1200	arg1	anteiso-C15 					1207:1218	anteiso-C15 	1207:1218	anteiso-C15 	1207:1218	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	6	19	theme	above	705:709	arg1	results					711:717	The above results	701:717	The above results	701:717	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	6	20	dep	strains	732:738	arg1	FJAT-49731					768:777	FJAT-49731	768:777	FJAT-49731	768:777	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	6	20	dep	strains	732:738	arg1	strains					732:738	strains FJAT-49754T, FJAT-49682 and FJAT-49731	732:777	strains FJAT-49754T, FJAT-49682 and FJAT-49731	732:777	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	6	20	dep	strains	732:738	arg1	FJAT-49682					753:762	FJAT-49682	753:762	FJAT-49682	753:762	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	6	20	dep	strains	732:738	arg1	FJAT-49754T					740:750	FJAT-49754T	740:750	FJAT-49754T	740:750	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	5	21	theme	genus	594:598	arg1	Lederbergia					600:610	the genus Lederbergia	590:610	the genus Lederbergia	590:610	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	7	22	theme	optimum	886:892	arg1	%					986:986	optimum at 1 %	973:986	optimum at 1 %	973:986	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	22	theme	optimum	886:892	arg1	w/v					967:969	w/v	967:969	w/v	967:969	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	22	theme	optimum	886:892	arg1	%					964:964	up to 7 %	956:964	optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %)	886:987	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	8	23	theme	strain	1032:1037	arg1	FJAT-49754T					1039:1049	strain FJAT-49754T	1032:1049	strain FJAT-49754T	1032:1049	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	11	24	theme	name	1426:1429	arg1	sp					1450:1451	the name Lederbergia citrea sp	1422:1451	the name Lederbergia citrea sp	1422:1451	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	0	25	theme	citrea	12:17	arg1	sp					19:20	Lederbergia citrea sp	0:20	Lederbergia citrea sp.	0:21	Lederbergia citrea sp.
37129938	3	26	theme	sequence	241:248	arg1	similarity					250:259	the highest 16S rRNA gene sequence similarity	215:259	the highest 16S rRNA gene sequence similarity	215:259	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	10	27	theme	DNA	1262:1264	arg1	content					1270:1276	The genomic DNA G+C content	1250:1276	The genomic DNA G+C content of strain FJAT-49754T	1250:1298	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	10	27	theme	DNA	1262:1264	arg1	%					1309:1309	38.7 %	1304:1309	38.7 %	1304:1309	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	9	28	theme	strain	1183:1188	arg1	FJAT-49754T					1190:1200	strain FJAT-49754T	1183:1200	strain FJAT-49754T	1183:1200	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	13	29	theme	2019211T=LMG	1515:1526	arg1	FJAT-49754T					1492:1502	FJAT-49754T	1492:1502	FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T)	1492:1534	The type strain is FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T).
37129938	13	29	theme	2019211T=LMG	1515:1526	arg1	31589T					1528:1533	=CCTCC AB 2019211T=LMG 31589T	1505:1533	=CCTCC AB 2019211T=LMG 31589T	1505:1533	The type strain is FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T).
37129938	0	30	theme	Lederbergia	0:10	arg1	sp					19:20	Lederbergia citrea sp	0:20	Lederbergia citrea sp.	0:21	Lederbergia citrea sp.
37129938	5	31	theme	ANI	559:561	arg1	values					563:568	the ANI values	555:568	the ANI values with the members of the genus Lederbergia	555:610	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	11	32	theme	strain	1340:1345	arg1	FJAT-49754T					1347:1357	strain FJAT-49754T	1340:1357	strain FJAT-49754T	1340:1357	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	10	33	theme	strain	1281:1286	arg1	FJAT-49754T					1288:1298	strain FJAT-49754T	1281:1298	strain FJAT-49754T	1281:1298	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	5	34	with	values	563:568	arg1	members					579:585	the members	575:585	the members of the genus Lederbergia	575:610	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	35	theme	cut-off	650:656	arg1	level					658:662	the cut-off level	646:662	the cut-off level for prokaryotic species delineation	646:698	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	8	36	located	detected	1020:1027	arg2	menaquinone					1008:1018	the only menaquinone	999:1018	the only menaquinone detected in strain FJAT-49754T	999:1049	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	8	36	located	detected	1020:1027	arg1	FJAT-49754T					1039:1049	strain FJAT-49754T	1032:1049	strain FJAT-49754T	1032:1049	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	8	36	located	detected	1020:1027	arg2	MK-7					990:993	MK-7	990:993	MK-7	990:993	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	5	37	theme	ANI	465:467	arg1	values					470:475	The average nucleotide identity (ANI) values	432:475	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731	432:530	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	37	theme	ANI	465:467	arg1	%					546:546	above 96 %	537:546	above 96 %	537:546	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	7	38	from	pH	928:929	arg1	at					925:926	optimum at pH 8.0	917:933	optimum at pH 8.0	917:933	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	11	39	theme	above	1325:1329	arg1	results					1331:1337	the above results	1321:1337	the above results	1321:1337	Based on the above results, strain FJAT-49754T represents a novel species of the genus Lederbergia, for which the name Lederbergia citrea sp.
37129938	3	40	theme	Lederbergia	285:295	arg1	panacisoli					297:306	Lederbergia panacisoli	285:306	Lederbergia panacisoli (97.8-97.9 %)	285:320	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	3	40	theme	Lederbergia	285:295	arg1	%					319:319	97.8-97.9 %	309:319	97.8-97.9 %	309:319	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	7	41	dep	°C	882:883	arg1	%					986:986	optimum at 1 %	973:986	optimum at 1 %	973:986	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	41	dep	°C	882:883	arg1	w/v					967:969	w/v	967:969	w/v	967:969	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	41	dep	°C	882:883	arg1	%					964:964	up to 7 %	956:964	optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %)	886:987	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	8	42	theme	main	1060:1063	arg1	diphosphatidylglycerol					1083:1104	diphosphatidylglycerol	1083:1104	diphosphatidylglycerol	1083:1104	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	8	42	theme	main	1060:1063	arg1	lipids					1071:1076	the main polar lipids	1056:1076	the main polar lipids	1056:1076	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	5	43	theme	Lederbergia	600:610	arg1	members					579:585	the members	575:585	the members of the genus Lederbergia	575:610	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	3	44	theme	type	270:273	arg1	strain					275:280	the type strain	266:280	the type strain of Lederbergia panacisoli (97.8-97.9 %)	266:320	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	6	45	theme	Lederbergia	818:828	arg1	species					797:803	a novel species	789:803	a novel species of the genus Lederbergia	789:828	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	5	46	theme	prokaryotic	668:678	arg1	delineation					688:698	prokaryotic species delineation	668:698	prokaryotic species delineation	668:698	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	2	47	theme	soil	181:184	arg1	sample					186:191	the citrus rhizosphere soil sample	158:191	the citrus rhizosphere soil sample	158:191	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	4	48	theme	gene	336:339	arg1	%					429:429	99.9 %	424:429	99.9 %	424:429	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	4	48	theme	gene	336:339	arg1	similarities					350:361	The 16S rRNA gene sequence similarities	323:361	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731	323:417	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	9	49	dep	anteiso-C15 	1207:1218	arg1	 0					1233:1234	 0	1233:1234	 0	1233:1234	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	9	49	dep	anteiso-C15 	1207:1218	arg1	C16 					1241:1244	C16 	1241:1244	C16 	1241:1244	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	9	49	dep	anteiso-C15 	1207:1218	arg1	 0					1220:1221	 0	1220:1221	 0	1220:1221	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	13	50	theme	=CCTCC AB	1505:1513	arg1	FJAT-49754T					1492:1502	FJAT-49754T	1492:1502	FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T)	1492:1534	The type strain is FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T).
37129938	13	50	theme	=CCTCC AB	1505:1513	arg1	31589T					1528:1533	=CCTCC AB 2019211T=LMG 31589T	1505:1533	=CCTCC AB 2019211T=LMG 31589T	1505:1533	The type strain is FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T).
37129938	2	51	theme	rhizosphere	169:179	arg1	sample					186:191	the citrus rhizosphere soil sample	158:191	the citrus rhizosphere soil sample	158:191	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	4	52	theme	rRNA	331:334	arg1	%					429:429	99.9 %	424:429	99.9 %	424:429	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	4	52	theme	rRNA	331:334	arg1	similarities					350:361	The 16S rRNA gene sequence similarities	323:361	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731	323:417	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	8	53	theme	polar	1065:1069	arg1	diphosphatidylglycerol					1083:1104	diphosphatidylglycerol	1083:1104	diphosphatidylglycerol	1083:1104	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	8	53	theme	polar	1065:1069	arg1	lipids					1071:1076	the main polar lipids	1056:1076	the main polar lipids	1056:1076	MK-7 was the only menaquinone detected in strain FJAT-49754T, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine.
37129938	9	54	theme	major	1162:1166	arg1	acids					1174:1178	The major fatty acids	1158:1178	The major fatty acids of strain FJAT-49754T	1158:1200	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	9	54	theme	major	1162:1166	arg1	anteiso-C15 					1207:1218	anteiso-C15 	1207:1218	anteiso-C15 	1207:1218	The major fatty acids of strain FJAT-49754T were anteiso-C15 : 0, iso-C15 : 0, and C16 : 0.
37129938	10	55	theme	genomic	1254:1260	arg1	content					1270:1276	The genomic DNA G+C content	1250:1276	The genomic DNA G+C content of strain FJAT-49754T	1250:1298	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	10	55	theme	genomic	1254:1260	arg1	%					1309:1309	38.7 %	1304:1309	38.7 %	1304:1309	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	6	56	theme	genus	812:816	arg1	Lederbergia					818:828	the genus Lederbergia	808:828	the genus Lederbergia	808:828	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	2	57	theme	Gram-positive-staining	69:90	arg1	FJAT-49731					128:137	FJAT-49731	128:137	FJAT-49731	128:137	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	57	theme	Gram-positive-staining	69:90	arg1	strains					92:98	Three Gram-positive-staining strains	63:98	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731	63:137	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	57	theme	Gram-positive-staining	69:90	arg1	FJAT-49682					113:122	FJAT-49682	113:122	FJAT-49682	113:122	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	57	theme	Gram-positive-staining	69:90	arg1	FJAT-49754T					100:110	FJAT-49754T	100:110	FJAT-49754T	100:110	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	3	58	theme	panacisoli	297:306	arg1	strain					275:280	the type strain	266:280	the type strain of Lederbergia panacisoli (97.8-97.9 %)	266:320	These strains showed the highest 16S rRNA gene sequence similarity with the type strain of Lederbergia panacisoli (97.8-97.9 %).
37129938	10	59	theme	G+C	1266:1268	arg1	content					1270:1276	The genomic DNA G+C content	1250:1276	The genomic DNA G+C content of strain FJAT-49754T	1250:1298	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	10	59	theme	G+C	1266:1268	arg1	%					1309:1309	38.7 %	1304:1309	38.7 %	1304:1309	The genomic DNA G+C content of strain FJAT-49754T was 38.7 %.
37129938	4	60	theme	sequence	341:348	arg1	%					429:429	99.9 %	424:429	99.9 %	424:429	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	4	60	theme	sequence	341:348	arg1	similarities					350:361	The 16S rRNA gene sequence similarities	323:361	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731	323:417	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	2	61	attach	isolated	144:151	arg1	sample					186:191	the citrus rhizosphere soil sample	158:191	the citrus rhizosphere soil sample	158:191	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	61	attach	isolated	144:151	arg2	FJAT-49682					113:122	FJAT-49682	113:122	FJAT-49682	113:122	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	61	attach	isolated	144:151	arg2	strains					92:98	Three Gram-positive-staining strains	63:98	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731	63:137	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	61	attach	isolated	144:151	arg2	FJAT-49754T					100:110	FJAT-49754T	100:110	FJAT-49754T	100:110	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	61	attach	isolated	144:151	arg2	FJAT-49731					128:137	FJAT-49731	128:137	FJAT-49731	128:137	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	5	62	theme	average	436:442	arg1	values					470:475	The average nucleotide identity (ANI) values	432:475	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731	432:530	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	62	theme	average	436:442	arg1	%					546:546	above 96 %	537:546	above 96 %	537:546	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	63	dep	strains	485:491	arg1	FJAT-49731					521:530	FJAT-49731	521:530	FJAT-49731	521:530	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	63	dep	strains	485:491	arg1	strains					485:491	strains FJAT-49754T, FJAT-49682 and FJAT-49731	485:530	strains FJAT-49754T, FJAT-49682 and FJAT-49731	485:530	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	63	dep	strains	485:491	arg1	FJAT-49682					506:515	FJAT-49682	506:515	FJAT-49682	506:515	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	63	dep	strains	485:491	arg1	FJAT-49754T					493:503	FJAT-49754T	493:503	FJAT-49754T	493:503	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	6	64	theme	novel	791:795	arg1	species					797:803	a novel species	789:803	a novel species of the genus Lederbergia	789:828	The above results suggest that strains FJAT-49754T, FJAT-49682 and FJAT-49731 belong to a novel species of the genus Lederbergia.
37129938	7	65	located	observed	864:871	arg2	Growth					831:836	Growth	831:836	Growth of strain FJAT-49754T	831:858	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	65	located	observed	864:871	arg1	°C					882:883	10-40 °C	876:883	10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %)	876:987	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	66	theme	optimum	917:923	arg1	pH					928:929	optimum at pH 8.0	917:933	optimum at pH 8.0	917:933	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	66	theme	optimum	917:923	arg1	pH					904:905	pH 6.0-10.0	904:914	pH 6.0-10.0 (optimum at pH 8.0)	904:934	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	5	67	theme	nucleotide	444:453	arg1	values					470:475	The average nucleotide identity (ANI) values	432:475	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731	432:530	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	67	theme	nucleotide	444:453	arg1	%					546:546	above 96 %	537:546	above 96 %	537:546	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	7	68	theme	at	925:926	arg1	pH					928:929	optimum at pH 8.0	917:933	optimum at pH 8.0	917:933	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	68	theme	at	925:926	arg1	pH					904:905	pH 6.0-10.0	904:914	pH 6.0-10.0 (optimum at pH 8.0)	904:934	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	13	69	theme	type	1477:1480	arg1	FJAT-49754T					1492:1502	FJAT-49754T	1492:1502	FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T)	1492:1534	The type strain is FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T).
37129938	13	69	theme	type	1477:1480	arg1	strain					1482:1487	The type strain	1473:1487	The type strain	1473:1487	The type strain is FJAT-49754T (=CCTCC AB 2019211T=LMG 31589T).
37129938	5	70	theme	identity	455:462	arg1	values					470:475	The average nucleotide identity (ANI) values	432:475	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731	432:530	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	5	70	theme	identity	455:462	arg1	%					546:546	above 96 %	537:546	above 96 %	537:546	The average nucleotide identity (ANI) values between strains FJAT-49754T, FJAT-49682 and FJAT-49731 were above 96 %, while the ANI values with the members of the genus Lederbergia were below 95 %, which were below the cut-off level for prokaryotic species delineation.
37129938	7	71	from	tolerance	946:954	arg1	optimum					886:892	optimum	886:892	optimum	886:892	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	72	dep	7 	962:963	arg1	to					959:960	to	959:960	to	959:960	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	2	73	dep	strains	92:98	arg1	FJAT-49731					128:137	FJAT-49731	128:137	FJAT-49731	128:137	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	73	dep	strains	92:98	arg1	strains					92:98	Three Gram-positive-staining strains	63:98	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731	63:137	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	73	dep	strains	92:98	arg1	FJAT-49682					113:122	FJAT-49682	113:122	FJAT-49682	113:122	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	2	73	dep	strains	92:98	arg1	FJAT-49754T					100:110	FJAT-49754T	100:110	FJAT-49754T	100:110	Three Gram-positive-staining strains FJAT-49754T, FJAT-49682 and FJAT-49731 were isolated from the citrus rhizosphere soil sample.
37129938	4	74	theme	16S	327:329	arg1	%					429:429	99.9 %	424:429	99.9 %	424:429	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	4	74	theme	16S	327:329	arg1	similarities					350:361	The 16S rRNA gene sequence similarities	323:361	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731	323:417	The 16S rRNA gene sequence similarities between strains FJAT-49754T, FJAT-49682, and FJAT-49731 were 99.9 %.
37129938	7	75	from	°C	900:901	arg1	optimum					886:892	optimum	886:892	optimum	886:892	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
37129938	7	76	theme	strain	841:846	arg1	FJAT-49754T					848:858	strain FJAT-49754T	841:858	strain FJAT-49754T	841:858	Growth of strain FJAT-49754T was observed at 10-40 °C (optimum at 30 °C, pH 6.0-10.0 (optimum at pH 8.0), and NaCl tolerance up to 7 % (w/v) (optimum at 1 %).
36334279	4	0	theme	indigenous	626:635	arg1	NCDC					671:674	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	5	1	dep	either	863:868	arg1	independently					870:882	independently	870:882	independently	870:882	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	4	2	theme	Lactobacillus	647:659	arg1	NCDC					671:674	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	10	3	theme	indigenous	1619:1628	arg1	NCDC					1664:1667	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	7	4	theme	HDL	1164:1166	arg1	cholesterol					1168:1178	HDL cholesterol	1164:1178	HDL cholesterol going up and LDL cholesterol and triglyceride levels going down	1164:1242	The lipid profile changed, with HDL cholesterol going up and LDL cholesterol and triglyceride levels going down.
36334279	0	5	theme	prebiotic	35:43	arg1	milk					55:58	probiotic and prebiotic fermented milk	21:58	probiotic and prebiotic fermented milk	21:58	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	9	6	theme	L.	1461:1462	arg1	NCDC					1474:1477	L. fermentum NCDC 400	1461:1481	L. fermentum NCDC 400 alone	1461:1487	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	5	7	dep	NCDC	854:857	arg1	conjunction					890:900	conjunction	890:900	conjunction with FOS	890:909	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	5	7	dep	NCDC	854:857	arg1	either					863:868	either	863:868	either	863:868	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	10	8	theme	preventive	1697:1706	arg1	role					1708:1711	a preventive role	1695:1711	a preventive role	1695:1711	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	8	9	theme	FOS	1280:1282	arg1	combinations					1284:1295	FOS combinations	1280:1295	FOS combinations	1280:1295	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	0	10	theme	Protective	0:9	arg1	effect					11:16	Protective effect	0:16	Protective effect of probiotic and prebiotic fermented milk	0:58	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	9	11	theme	NCDC	1474:1477	arg1	consumption					1446:1456	consumption	1446:1456	consumption of L. fermentum NCDC 400 alone or its combinational effects	1446:1516	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	10	12	from	properties	1798:1807	arg1	mice					1826:1829	HFD-fed obese mice	1812:1829	HFD-fed obese mice	1812:1829	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	4	13	theme	hepatic	745:751	arg1	steatosis					753:761	obesity-associated hepatic steatosis	726:761	obesity-associated hepatic steatosis	726:761	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	11	14	theme	hepatic	1891:1897	arg1	steatosis					1899:1907	obesity-associated hepatic steatosis	1872:1907	obesity-associated hepatic steatosis	1872:1907	Our finding would be helpful to prevent obesity-associated hepatic steatosis and inflammation upon supplementation of pre- and pro-biotics (synbiotics).
36334279	5	15	theme	body	932:935	arg1	weight					937:942	reduced body weight	924:942	reduced body weight	924:942	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	8	16	theme	glucose	1315:1321	arg1	levels					1372:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels	1307:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD	1307:1398	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	10	17	theme	Lactobacillus	1640:1652	arg1	NCDC					1664:1667	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	8	18	theme	fermentum	1257:1265	arg1	NCDC					1267:1270	L. fermentum NCDC 400	1254:1274	L. fermentum NCDC 400	1254:1274	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	8	19	theme	gHbA1c	1324:1329	arg1	levels					1372:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels	1307:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD	1307:1398	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	9	20	theme	effects	1510:1516	arg1	consumption					1446:1456	consumption	1446:1456	consumption of L. fermentum NCDC 400 alone or its combinational effects	1446:1516	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	6	21	theme	anti-oxidative	1005:1018	arg1	activity					1027:1034	the anti-oxidative enzyme activity	1001:1034	the anti-oxidative enzyme activity	1001:1034	Also, the anti-oxidative enzyme activity went down, and the inflammatory profile got better, with less fat getting into the hepatocytes.
36334279	8	22	theme	gastric	1332:1338	arg1	peptide					1351:1357	gastric inhibitory peptide	1332:1357	gastric inhibitory peptide	1332:1357	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	2	23	theme	complex	246:252	arg1	pathophysiology					254:268	The complex pathophysiology	242:268	The complex pathophysiology of obesity	242:279	The complex pathophysiology of obesity explains the difficulty provided to health policy for its clinical management.
36334279	5	24	theme	treatment	984:992	arg1	24 weeks					972:979	24 weeks	972:979	24 weeks of treatment	972:992	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	8	25	theme	peptide	1351:1357	arg1	levels					1372:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels	1307:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD	1307:1398	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	10	26	dep	APPLICATIONS	1591:1602	arg1	improves					1754:1761	improves	1754:1761	improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice	1754:1829	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	10	26	dep	APPLICATIONS	1591:1602	arg1	had					1691:1693	had	1691:1693	had a preventive role in obesity-induced hepatic steatosis	1691:1748	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	3	27	from	differences	450:460	arg1	consumption					465:475	consumption	465:475	consumption of probiotics, its relevance to gut microbiota activity and composition	465:547	Increasing data show that obesity and metabolic abnormalities are intimately connected to differences in consumption of probiotics, its relevance to gut microbiota activity and composition.
36334279	7	28	theme	triglyceride	1213:1224	arg1	levels					1226:1231	LDL cholesterol and triglyceride levels	1193:1231	LDL cholesterol and triglyceride levels	1193:1231	The lipid profile changed, with HDL cholesterol going up and LDL cholesterol and triglyceride levels going down.
36334279	9	29	theme	obesity-associated	1543:1560	arg1	steatosis					1570:1578	obesity-associated hepatic steatosis	1543:1578	obesity-associated hepatic steatosis	1543:1578	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	3	30	theme	Increasing	360:369	arg1	data					371:374	Increasing data	360:374	Increasing data	360:374	Increasing data show that obesity and metabolic abnormalities are intimately connected to differences in consumption of probiotics, its relevance to gut microbiota activity and composition.
36334279	6	31	theme	inflammatory	1055:1066	arg1	got					1076:1078	the inflammatory profile got	1051:1078	the inflammatory profile got	1051:1078	Also, the anti-oxidative enzyme activity went down, and the inflammatory profile got better, with less fat getting into the hepatocytes.
36334279	3	32	theme	metabolic	398:406	arg1	abnormalities					408:420	metabolic abnormalities	398:420	metabolic abnormalities	398:420	Increasing data show that obesity and metabolic abnormalities are intimately connected to differences in consumption of probiotics, its relevance to gut microbiota activity and composition.
36334279	0	33	theme	obesity-induced	103:117	arg1	steatosis					127:135	obesity-induced hepatic steatosis	103:135	obesity-induced hepatic steatosis	103:135	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	4	34	theme	high-fat	794:801	arg1	HFD					809:811	HFD	809:811	HFD	809:811	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	4	34	theme	high-fat	794:801	arg1	diet					803:806	a high-fat diet	792:806	a high-fat diet (HFD)	792:812	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	7	35	dep	up	1186:1187	arg1	down					1239:1242	going down	1233:1242	going down	1233:1242	The lipid profile changed, with HDL cholesterol going up and LDL cholesterol and triglyceride levels going down.
36334279	5	36	theme	L.	841:842	arg1	NCDC					854:857	L. fermentum NCDC 400	841:861	L. fermentum NCDC 400 either independently or in conjunction with FOS	841:909	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	8	37	from	levels	1372:1377	arg1	mice					1382:1385	mice	1382:1385	mice fed with HFD	1382:1398	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	0	38	theme	Lactobacillus	71:83	arg1	fermentum					85:93	Lactobacillus fermentum	71:93	Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation	71:152	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	3	39	theme	probiotics	480:489	arg1	consumption					465:475	consumption	465:475	consumption of probiotics, its relevance to gut microbiota activity and composition	465:547	Increasing data show that obesity and metabolic abnormalities are intimately connected to differences in consumption of probiotics, its relevance to gut microbiota activity and composition.
36334279	7	40	theme	going	1233:1237	arg1	down					1239:1242	going down	1233:1242	going down	1233:1242	The lipid profile changed, with HDL cholesterol going up and LDL cholesterol and triglyceride levels going down.
36334279	3	41	theme	microbiota	513:522	arg1	activity					524:531	gut microbiota activity	509:531	gut microbiota activity	509:531	Increasing data show that obesity and metabolic abnormalities are intimately connected to differences in consumption of probiotics, its relevance to gut microbiota activity and composition.
36334279	4	42	from	effect	599:604	arg1	inflammation					767:778	inflammation	767:778	inflammation	767:778	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	4	42	from	effect	599:604	arg1	steatosis					753:761	obesity-associated hepatic steatosis	726:761	obesity-associated hepatic steatosis	726:761	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	6	43	theme	getting	1102:1108	arg1	fat					1098:1100	less fat getting	1093:1108	less fat getting into the hepatocytes	1093:1129	Also, the anti-oxidative enzyme activity went down, and the inflammatory profile got better, with less fat getting into the hepatocytes.
36334279	4	44	theme	oral	609:612	arg1	delivery					614:621	oral delivery	609:621	oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS)	609:721	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	5	45	with	conjunction	890:900	arg1	FOS					907:909	FOS	907:909	FOS	907:909	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	10	46	theme	hepatic	1732:1738	arg1	steatosis					1740:1748	obesity-induced hepatic steatosis	1716:1748	obesity-induced hepatic steatosis	1716:1748	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	9	47	theme	fermentum	1464:1472	arg1	NCDC					1474:1477	L. fermentum NCDC 400	1461:1481	L. fermentum NCDC 400 alone	1461:1487	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	5	48	theme	fermentum	844:852	arg1	NCDC					854:857	L. fermentum NCDC 400	841:861	L. fermentum NCDC 400 either independently or in conjunction with FOS	841:909	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	10	49	theme	anti-inflammatory	1780:1796	arg1	properties					1798:1807	anti-inflammatory properties	1780:1807	anti-inflammatory properties	1780:1807	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	0	50	theme	fermented	45:53	arg1	milk					55:58	probiotic and prebiotic fermented milk	21:58	probiotic and prebiotic fermented milk	21:58	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	1	51	theme	epidemic	175:182	arg1	proportions					184:194	epidemic proportions	175:194	epidemic proportions	175:194	Obesity has reached epidemic proportions, with major economic and health implications.
36334279	4	52	theme	fermentum	661:669	arg1	NCDC					671:674	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	10	53	theme	obesity-induced	1716:1730	arg1	steatosis					1740:1748	obesity-induced hepatic steatosis	1716:1748	obesity-induced hepatic steatosis	1716:1748	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	10	54	theme	obese	1820:1824	arg1	mice					1826:1829	HFD-fed obese mice	1812:1829	HFD-fed obese mice	1812:1829	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	1	55	theme	major	202:206	arg1	economic					208:215	major economic	202:215	major economic	202:215	Obesity has reached epidemic proportions, with major economic and health implications.
36334279	10	56	contain	had	1691:1693	arg1	NCDC					1664:1667	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	10	56	contain	had	1691:1693	arg1	FOS					1687:1689	prebiotic FOS	1677:1689	prebiotic FOS	1677:1689	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	10	56	contain	had	1691:1693	arg2	role					1708:1711	a preventive role	1695:1711	a preventive role	1695:1711	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	10	57	theme	PRACTICAL	1581:1589	arg1	APPLICATIONS					1591:1602	PRACTICAL APPLICATIONS	1581:1602	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.	1581:1830	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	10	58	from	anti-oxidant	1763:1774	arg1	mice					1826:1829	HFD-fed obese mice	1812:1829	HFD-fed obese mice	1812:1829	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	10	59	theme	probiotic	1630:1638	arg1	NCDC					1664:1667	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	4	60	theme	probiotic	637:645	arg1	NCDC					671:674	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	indigenous probiotic Lactobacillus fermentum NCDC 400	626:678	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	10	61	theme	potential	1609:1617	arg1	NCDC					1664:1667	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	2	62	theme	health	317:322	arg1	policy					324:329	health policy	317:329	health policy	317:329	The complex pathophysiology of obesity explains the difficulty provided to health policy for its clinical management.
36334279	5	63	theme	reduced	924:930	arg1	weight					937:942	reduced body weight	924:942	reduced body weight	924:942	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	7	64	theme	lipid	1136:1140	arg1	profile					1142:1148	The lipid profile	1132:1148	The lipid profile	1132:1148	The lipid profile changed, with HDL cholesterol going up and LDL cholesterol and triglyceride levels going down.
36334279	7	65	theme	cholesterol	1197:1207	arg1	levels					1226:1231	LDL cholesterol and triglyceride levels	1193:1231	LDL cholesterol and triglyceride levels	1193:1231	The lipid profile changed, with HDL cholesterol going up and LDL cholesterol and triglyceride levels going down.
36334279	4	66	theme	obesity-associated	726:743	arg1	steatosis					753:761	obesity-associated hepatic steatosis	726:761	obesity-associated hepatic steatosis	726:761	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	10	67	theme	fermentum	1654:1662	arg1	NCDC					1664:1667	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	The potential indigenous probiotic Lactobacillus fermentum NCDC 400	1605:1671	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	2	68	theme	clinical	339:346	arg1	management					348:357	its clinical management	335:357	its clinical management	335:357	The complex pathophysiology of obesity explains the difficulty provided to health policy for its clinical management.
36334279	1	69	theme	health	221:226	arg1	implications					228:239	health implications	221:239	health implications	221:239	Obesity has reached epidemic proportions, with major economic and health implications.
36334279	4	70	theme	prebiotic	684:692	arg1	FOS					718:720	FOS	718:720	FOS	718:720	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	4	70	theme	prebiotic	684:692	arg1	fructo-oligosaccharide					694:715	prebiotic fructo-oligosaccharide	684:715	prebiotic fructo-oligosaccharide (FOS)	684:721	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	11	71	theme	obesity-associated	1872:1889	arg1	steatosis					1899:1907	obesity-associated hepatic steatosis	1872:1907	obesity-associated hepatic steatosis	1872:1907	Our finding would be helpful to prevent obesity-associated hepatic steatosis and inflammation upon supplementation of pre- and pro-biotics (synbiotics).
36334279	2	72	theme	obesity	273:279	arg1	pathophysiology					254:268	The complex pathophysiology	242:268	The complex pathophysiology of obesity	242:279	The complex pathophysiology of obesity explains the difficulty provided to health policy for its clinical management.
36334279	9	73	theme	combinational	1496:1508	arg1	effects					1510:1516	its combinational effects	1492:1516	its combinational effects	1492:1516	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	8	74	theme	L.	1254:1255	arg1	NCDC					1267:1270	L. fermentum NCDC 400	1254:1274	L. fermentum NCDC 400	1254:1274	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	6	75	theme	enzyme	1020:1025	arg1	activity					1027:1034	the anti-oxidative enzyme activity	1001:1034	the anti-oxidative enzyme activity	1001:1034	Also, the anti-oxidative enzyme activity went down, and the inflammatory profile got better, with less fat getting into the hepatocytes.
36334279	10	76	theme	prebiotic	1677:1685	arg1	FOS					1687:1689	prebiotic FOS	1677:1689	prebiotic FOS	1677:1689	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
36334279	9	77	theme	protective	1524:1533	arg1	role					1535:1538	a protective role	1522:1538	a protective role	1522:1538	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	8	78	theme	inhibitory	1340:1349	arg1	peptide					1351:1357	gastric inhibitory peptide	1332:1357	gastric inhibitory peptide	1332:1357	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	5	79	theme	C57BL/6	815:821	arg1	mice					823:826	C57BL/6 mice	815:826	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS	815:909	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	4	80	theme	fructo-oligosaccharide	694:715	arg1	delivery					614:621	oral delivery	609:621	oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS)	609:721	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	5	81	theme	abdominal	948:956	arg1	obesity					958:964	abdominal obesity	948:964	abdominal obesity	948:964	C57BL/6 mice treated with L. fermentum NCDC 400 either independently or in conjunction with FOS demonstrated reduced body weight and abdominal obesity after 24 weeks of treatment.
36334279	9	82	theme	hepatic	1562:1568	arg1	steatosis					1570:1578	obesity-associated hepatic steatosis	1543:1578	obesity-associated hepatic steatosis	1543:1578	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	6	83	theme	profile	1068:1074	arg1	got					1076:1078	the inflammatory profile got	1051:1078	the inflammatory profile got	1051:1078	Also, the anti-oxidative enzyme activity went down, and the inflammatory profile got better, with less fat getting into the hepatocytes.
36334279	4	84	theme	investigation	567:579	arg1	goal					554:557	The goal	550:557	The goal of this investigation	550:579	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	8	85	theme	insulin	1364:1370	arg1	levels					1372:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels	1307:1377	fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD	1307:1398	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	0	86	theme	hepatic	119:125	arg1	steatosis					127:135	obesity-induced hepatic steatosis	103:135	obesity-induced hepatic steatosis	103:135	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	8	87	theme	fasting	1307:1313	arg1	glucose					1315:1321	fasting glucose	1307:1321	fasting glucose	1307:1321	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	0	88	theme	probiotic	21:29	arg1	milk					55:58	probiotic and prebiotic fermented milk	21:58	probiotic and prebiotic fermented milk	21:58	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	4	89	theme	delivery	614:621	arg1	effect					599:604	the effect	595:604	the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD)	595:812	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	7	90	theme	LDL	1193:1195	arg1	cholesterol					1197:1207	LDL cholesterol	1193:1207	LDL cholesterol	1193:1207	The lipid profile changed, with HDL cholesterol going up and LDL cholesterol and triglyceride levels going down.
36334279	9	91	contain	had	1518:1520	arg1	consumption					1446:1456	consumption	1446:1456	consumption of L. fermentum NCDC 400 alone or its combinational effects	1446:1516	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	9	91	contain	had	1518:1520	arg2	role					1535:1538	a protective role	1522:1538	a protective role	1522:1538	Overall, consumption of L. fermentum NCDC 400 alone or its combinational effects had a protective role on obesity-associated hepatic steatosis.
36334279	8	92	theme	glucose	1416:1422	arg1	homeostasis					1424:1434	glucose homeostasis	1416:1434	glucose homeostasis	1416:1434	Further, L. fermentum NCDC 400 and FOS combinations decreased fasting glucose, gHbA1c, gastric inhibitory peptide, and insulin levels in mice fed with HFD, thus improving glucose homeostasis.
36334279	3	93	theme	gut	509:511	arg1	activity					524:531	gut microbiota activity	509:531	gut microbiota activity	509:531	Increasing data show that obesity and metabolic abnormalities are intimately connected to differences in consumption of probiotics, its relevance to gut microbiota activity and composition.
36334279	0	94	theme	milk	55:58	arg1	effect					11:16	Protective effect	0:16	Protective effect of probiotic and prebiotic fermented milk	0:58	Protective effect of probiotic and prebiotic fermented milk containing Lactobacillus fermentum against obesity-induced hepatic steatosis and inflammation.
36334279	11	95	theme	pro-biotics	1959:1969	arg1	supplementation					1931:1945	supplementation	1931:1945	supplementation of pre- and pro-biotics (synbiotics)	1931:1982	Our finding would be helpful to prevent obesity-associated hepatic steatosis and inflammation upon supplementation of pre- and pro-biotics (synbiotics).
36334279	4	96	theme	NCDC	671:674	arg1	delivery					614:621	oral delivery	609:621	oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS)	609:721	The goal of this investigation was to assess the effect of oral delivery of indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic fructo-oligosaccharide (FOS) on obesity-associated hepatic steatosis and inflammation produced by a high-fat diet (HFD).
36334279	11	97	theme	pre-	1950:1953	arg1	supplementation					1931:1945	supplementation	1931:1945	supplementation of pre- and pro-biotics (synbiotics)	1931:1982	Our finding would be helpful to prevent obesity-associated hepatic steatosis and inflammation upon supplementation of pre- and pro-biotics (synbiotics).
36334279	10	98	theme	HFD-fed	1812:1818	arg1	mice					1826:1829	HFD-fed obese mice	1812:1829	HFD-fed obese mice	1812:1829	PRACTICAL APPLICATIONS: The potential indigenous probiotic Lactobacillus fermentum NCDC 400 and prebiotic FOS had a preventive role in obesity-induced hepatic steatosis and improves anti-oxidant and anti-inflammatory properties in HFD-fed obese mice.
35870569	7	0	theme	AnxV-Rb1-LPs	1262:1273	arg1	preparation					1247:1257	the final preparation	1237:1257	the final preparation of AnxV-Rb1-LPs	1237:1273	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	6	1	theme	similar	769:775	arg1	structure					777:785	a similar structure	767:785	a similar structure	767:785	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	8	2	theme	lipid	1387:1391	arg1	bilayer					1393:1399	the lipid bilayer	1383:1399	the lipid bilayer	1383:1399	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	11	3	with	diseases	2136:2143	arg1	accumulation					2172:2183	excessive cholesterol accumulation	2150:2183	excessive cholesterol accumulation	2150:2183	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	6	4	theme	cholesterol	963:973	arg1	solubilization					945:958	the solubilization	941:958	the solubilization of cholesterol in lipid bilayer	941:990	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	5	theme	nano-sponge-like	885:900	arg1	liposomes					902:910	nano-sponge-like liposomes	885:910	nano-sponge-like liposomes (Rb1-LPs)	885:920	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	5	theme	nano-sponge-like	885:900	arg1	Rb1-LPs					913:919	Rb1-LPs	913:919	Rb1-LPs	913:919	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	7	6	theme	atherosclerotic	1019:1033	arg1	plaques					1035:1041	atherosclerotic plaques	1019:1041	atherosclerotic plaques	1019:1041	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	11	7	theme	potential	2019:2027	arg1	strategy					2029:2036	a potential strategy	2017:2036	a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation	2017:2183	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	9	8	from	apoptosis	1644:1652	arg1	cells					1675:1679	cholesterol-laden cells	1657:1679	cholesterol-laden cells	1657:1679	Additionally, nano-sponge-like liposomes could efficiently reduce the accumulation of cholesterol crystals and improve cholesterol efflux, finally inhibiting inflammation and apoptosis in cholesterol-laden cells.
35870569	9	9	theme	nano-sponge-like	1483:1498	arg1	liposomes					1500:1508	nano-sponge-like liposomes	1483:1508	nano-sponge-like liposomes	1483:1508	Additionally, nano-sponge-like liposomes could efficiently reduce the accumulation of cholesterol crystals and improve cholesterol efflux, finally inhibiting inflammation and apoptosis in cholesterol-laden cells.
35870569	6	10	contain	has	763:765	arg1	components					735:744	main active components	723:744	main active components of ginseng	723:755	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	10	contain	has	763:765	arg1	one					716:718	one	716:718	one	716:718	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	10	contain	has	763:765	arg1	Rb1					705:707	ginsenosides Rb1	692:707	ginsenosides Rb1 (Rb1)	692:713	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	10	contain	has	763:765	arg2	structure					777:785	a similar structure	767:785	a similar structure	767:785	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	4	11	theme	bilayer	463:469	arg1	domain					447:452	the hydrophobic domain	431:452	the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol	431:538	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	4	11	theme	bilayer	463:469	arg1	bilayer					463:469	lipid bilayer	457:469	lipid bilayer	457:469	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	1	12	theme	Atherosclerotic	80:94	arg1	diseases					111:118	Atherosclerotic cardiovascular diseases	80:118	Atherosclerotic cardiovascular diseases	80:118	Atherosclerotic cardiovascular diseases remain the leading causes of morbidity and mortality worldwide.
35870569	6	13	theme	ginsenosides	692:703	arg1	components					735:744	main active components	723:744	main active components of ginseng	723:755	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	13	theme	ginsenosides	692:703	arg1	Rb1					710:712	Rb1	710:712	Rb1	710:712	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	13	theme	ginsenosides	692:703	arg1	one					716:718	one	716:718	one	716:718	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	13	theme	ginsenosides	692:703	arg1	Rb1					705:707	ginsenosides Rb1	692:707	ginsenosides Rb1 (Rb1)	692:713	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	11	14	theme	other	2130:2134	arg1	diseases					2136:2143	other diseases	2130:2143	other diseases with excessive cholesterol accumulation	2130:2183	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	5	15	theme	hydrophobic	627:637	arg1	capacity					656:663	its hydrophobic molecule loading capacity	623:663	its hydrophobic molecule loading capacity	623:663	Moreover, adjusting the composition of the lipid bilayer in liposomes can enhance its hydrophobic molecule loading capacity.
35870569	6	16	theme	hydrophobic	855:865	arg1	region					867:872	its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs)	851:920	its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs)	851:920	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	1	17	theme	cardiovascular	96:109	arg1	diseases					111:118	Atherosclerotic cardiovascular diseases	80:118	Atherosclerotic cardiovascular diseases	80:118	Atherosclerotic cardiovascular diseases remain the leading causes of morbidity and mortality worldwide.
35870569	6	18	theme	ginseng	749:755	arg1	components					735:744	main active components	723:744	main active components of ginseng	723:755	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	19	from	solubilization	945:958	arg1	bilayer					984:990	lipid bilayer	978:990	lipid bilayer	978:990	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	11	20	theme	excessive	2150:2158	arg1	accumulation					2172:2183	excessive cholesterol accumulation	2150:2183	excessive cholesterol accumulation	2150:2183	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	1	21	dep	morbidity	149:157	arg1	worldwide					173:181	worldwide	173:181	worldwide	173:181	Atherosclerotic cardiovascular diseases remain the leading causes of morbidity and mortality worldwide.
35870569	10	22	theme	nano-sponge-like	1799:1814	arg1	functions					1816:1824	nano-sponge-like functions	1799:1824	nano-sponge-like functions	1799:1824	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	6	23	with	bilayer	838:844	arg1	region					867:872	its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs)	851:920	its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs)	851:920	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	2	24	theme	Cholesterol	184:194	arg1	crystals					196:203	Cholesterol crystals	184:203	Cholesterol crystals in atherosclerotic plaques	184:230	Cholesterol crystals in atherosclerotic plaques play an essential role in atherosclerosis progression.
35870569	10	25	theme	intravenous	1770:1780	arg1	injection					1782:1790	intravenous injection	1770:1790	intravenous injection	1770:1790	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	8	26	theme	free	1404:1407	arg1	cholesterol					1409:1419	free cholesterol	1404:1419	free cholesterol	1404:1419	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	5	27	theme	lipid	584:588	arg1	bilayer					590:596	the lipid bilayer	580:596	the lipid bilayer	580:596	Moreover, adjusting the composition of the lipid bilayer in liposomes can enhance its hydrophobic molecule loading capacity.
35870569	9	28	from	inflammation	1627:1638	arg1	cells					1675:1679	cholesterol-laden cells	1657:1679	cholesterol-laden cells	1657:1679	Additionally, nano-sponge-like liposomes could efficiently reduce the accumulation of cholesterol crystals and improve cholesterol efflux, finally inhibiting inflammation and apoptosis in cholesterol-laden cells.
35870569	3	29	used	used	324:327	arg2	drugs					308:312	no clinical drugs	296:312	no clinical drugs	296:312	However, no clinical drugs have been used for removing cholesterol crystals from plaque to counter atherosclerosis.
35870569	11	30	from	crystals	2063:2070	arg1	plaques					2091:2097	atherosclerotic plaques	2075:2097	atherosclerotic plaques	2075:2097	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	7	31	theme	atherosclerotic	1138:1152	arg1	plaques					1154:1160	atherosclerotic plaques	1138:1160	atherosclerotic plaques	1138:1160	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	4	32	theme	hydrophobic	435:445	arg1	domain					447:452	the hydrophobic domain	431:452	the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol	431:538	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	4	32	theme	hydrophobic	435:445	arg1	bilayer					463:469	lipid bilayer	457:469	lipid bilayer	457:469	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	5	33	theme	bilayer	590:596	arg1	composition					565:575	the composition	561:575	the composition of the lipid bilayer in liposomes	561:609	Moreover, adjusting the composition of the lipid bilayer in liposomes can enhance its hydrophobic molecule loading capacity.
35870569	0	34	theme	Nano-sponge-like	0:15	arg1	liposomes					17:25	Nano-sponge-like liposomes	0:25	Nano-sponge-like liposomes	0:25	Nano-sponge-like liposomes remove cholesterol crystals for antiatherosclerosis.
35870569	10	35	theme	atherosclerotic	1740:1754	arg1	plaques					1756:1762	atherosclerotic plaques	1740:1762	atherosclerotic plaques	1740:1762	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	3	36	theme	clinical	299:306	arg1	drugs					308:312	no clinical drugs	296:312	no clinical drugs	296:312	However, no clinical drugs have been used for removing cholesterol crystals from plaque to counter atherosclerosis.
35870569	4	37	theme	lipid	457:461	arg1	bilayer					463:469	lipid bilayer	457:469	lipid bilayer	457:469	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	1	38	theme	leading	131:137	arg1	causes					139:144	the leading causes	127:144	the leading causes of morbidity and mortality worldwide	127:181	Atherosclerotic cardiovascular diseases remain the leading causes of morbidity and mortality worldwide.
35870569	0	39	theme	cholesterol	34:44	arg1	crystals					46:53	cholesterol crystals	34:53	cholesterol crystals for antiatherosclerosis	34:77	Nano-sponge-like liposomes remove cholesterol crystals for antiatherosclerosis.
35870569	6	40	theme	phospholipids	824:836	arg1	bilayer					838:844	soy phospholipids bilayer	820:844	soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs)	820:920	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	8	41	dep	in	1280:1281	arg1	vitro					1283:1287	vitro	1283:1287	vitro	1283:1287	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	6	42	theme	soy	820:822	arg1	bilayer					838:844	soy phospholipids bilayer	820:844	soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs)	820:920	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	4	43	from	domain	447:452	arg1	liposomes					474:482	liposomes	474:482	liposomes acted as sinks for solubilizing hydrophobic cholesterol	474:538	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	6	44	theme	components	735:744	arg1	components					735:744	main active components	723:744	main active components of ginseng	723:755	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	44	theme	components	735:744	arg1	one					716:718	one	716:718	one	716:718	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	6	44	theme	components	735:744	arg1	Rb1					705:707	ginsenosides Rb1	692:707	ginsenosides Rb1 (Rb1)	692:713	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	10	45	theme	intra-	1836:1841	arg1	crystals					1873:1880	intra- and extracellular cholesterol crystals	1836:1880	intra- and extracellular cholesterol crystals	1836:1880	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	11	46	theme	cholesterol	2160:2170	arg1	accumulation					2172:2183	excessive cholesterol accumulation	2150:2183	excessive cholesterol accumulation	2150:2183	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	9	47	theme	cholesterol-laden	1657:1673	arg1	cells					1675:1679	cholesterol-laden cells	1657:1679	cholesterol-laden cells	1657:1679	Additionally, nano-sponge-like liposomes could efficiently reduce the accumulation of cholesterol crystals and improve cholesterol efflux, finally inhibiting inflammation and apoptosis in cholesterol-laden cells.
35870569	8	48	theme	cholesterol	1447:1457	arg1	crystals					1459:1466	cholesterol crystals	1447:1466	cholesterol crystals	1447:1466	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	6	49	theme	active	728:733	arg1	components					735:744	main active components	723:744	main active components of ginseng	723:755	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	9	50	theme	cholesterol	1555:1565	arg1	crystals					1567:1574	cholesterol crystals	1555:1574	cholesterol crystals	1555:1574	Additionally, nano-sponge-like liposomes could efficiently reduce the accumulation of cholesterol crystals and improve cholesterol efflux, finally inhibiting inflammation and apoptosis in cholesterol-laden cells.
35870569	7	51	theme	Rb1-LPs	1201:1207	arg1	surface					1190:1196	the surface	1186:1196	the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs	1186:1273	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	10	52	theme	cholesterol	1861:1871	arg1	crystals					1873:1880	intra- and extracellular cholesterol crystals	1836:1880	intra- and extracellular cholesterol crystals	1836:1880	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	4	53	theme	hydrophobic	516:526	arg1	cholesterol					528:538	solubilizing hydrophobic cholesterol	503:538	solubilizing hydrophobic cholesterol	503:538	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	8	54	theme	crystals	1459:1466	arg1	solubilization					1429:1442	the solubilization	1425:1442	the solubilization of cholesterol crystals	1425:1466	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	8	54	theme	crystals	1459:1466	arg1	affinity					1371:1378	the affinity	1367:1378	the affinity of the lipid bilayer to free cholesterol	1367:1419	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	6	55	theme	main	723:726	arg1	components					735:744	main active components	723:744	main active components of ginseng	723:755	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35870569	8	56	theme	lipid	1332:1336	arg1	bilayer					1338:1344	lipid bilayer	1332:1344	lipid bilayer	1332:1344	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	5	57	from	composition	565:575	arg1	liposomes					601:609	liposomes	601:609	liposomes	601:609	Moreover, adjusting the composition of the lipid bilayer in liposomes can enhance its hydrophobic molecule loading capacity.
35870569	7	58	theme	final	1241:1245	arg1	preparation					1247:1257	the final preparation	1237:1257	the final preparation of AnxV-Rb1-LPs	1237:1273	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	8	59	theme	in	1280:1281	arg1	studies					1289:1295	The in vitro studies	1276:1295	The in vitro studies	1276:1295	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	2	60	theme	atherosclerotic	208:222	arg1	plaques					224:230	atherosclerotic plaques	208:230	atherosclerotic plaques	208:230	Cholesterol crystals in atherosclerotic plaques play an essential role in atherosclerosis progression.
35870569	9	61	theme	crystals	1567:1574	arg1	accumulation					1539:1550	the accumulation	1535:1550	the accumulation of cholesterol crystals	1535:1574	Additionally, nano-sponge-like liposomes could efficiently reduce the accumulation of cholesterol crystals and improve cholesterol efflux, finally inhibiting inflammation and apoptosis in cholesterol-laden cells.
35870569	4	62	theme	Previous	403:410	arg1	studies					412:418	Previous studies	403:418	Previous studies	403:418	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	1	63	theme	morbidity	149:157	arg1	causes					139:144	the leading causes	127:144	the leading causes of morbidity and mortality worldwide	127:181	Atherosclerotic cardiovascular diseases remain the leading causes of morbidity and mortality worldwide.
35870569	2	64	from	crystals	196:203	arg1	plaques					224:230	atherosclerotic plaques	208:230	atherosclerotic plaques	208:230	Cholesterol crystals in atherosclerotic plaques play an essential role in atherosclerosis progression.
35870569	7	65	theme	Annexin	1044:1050	arg1	V					1052:1052	Annexin V	1044:1052	Annexin V (AnxV)	1044:1059	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	7	65	theme	Annexin	1044:1050	arg1	protein					1064:1070	a protein	1062:1070	a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques	1062:1160	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	7	65	theme	Annexin	1044:1050	arg1	AnxV					1055:1058	AnxV	1055:1058	AnxV	1055:1058	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	2	66	theme	atherosclerosis	258:272	arg1	progression					274:284	atherosclerosis progression	258:284	atherosclerosis progression	258:284	Cholesterol crystals in atherosclerotic plaques play an essential role in atherosclerosis progression.
35870569	5	67	theme	molecule	639:646	arg1	capacity					656:663	its hydrophobic molecule loading capacity	623:663	its hydrophobic molecule loading capacity	623:663	Moreover, adjusting the composition of the lipid bilayer in liposomes can enhance its hydrophobic molecule loading capacity.
35870569	9	68	theme	cholesterol	1588:1598	arg1	efflux					1600:1605	cholesterol efflux	1588:1605	cholesterol efflux	1588:1605	Additionally, nano-sponge-like liposomes could efficiently reduce the accumulation of cholesterol crystals and improve cholesterol efflux, finally inhibiting inflammation and apoptosis in cholesterol-laden cells.
35870569	10	69	from	apoptosis	1923:1931	arg1	plaques					1952:1958	atherosclerotic plaques	1936:1958	atherosclerotic plaques	1936:1958	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	7	70	theme	click	1212:1216	arg1	reaction					1218:1225	click reaction	1212:1225	click reaction	1212:1225	For targeting delivery to atherosclerotic plaques, Annexin V (AnxV), a protein that can specifically recognize phosphatidylserine upregulated in atherosclerotic plaques, is applied to decorate the surface of Rb1-LPs by click reaction to obtain the final preparation of AnxV-Rb1-LPs.
35870569	3	71	theme	cholesterol	342:352	arg1	crystals					354:361	cholesterol crystals	342:361	cholesterol crystals	342:361	However, no clinical drugs have been used for removing cholesterol crystals from plaque to counter atherosclerosis.
35870569	11	72	theme	atherosclerotic	2075:2089	arg1	plaques					2091:2097	atherosclerotic plaques	2075:2097	atherosclerotic plaques	2075:2097	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	5	73	theme	loading	648:654	arg1	capacity					656:663	its hydrophobic molecule loading capacity	623:663	its hydrophobic molecule loading capacity	623:663	Moreover, adjusting the composition of the lipid bilayer in liposomes can enhance its hydrophobic molecule loading capacity.
35870569	1	74	theme	mortality	163:171	arg1	causes					139:144	the leading causes	127:144	the leading causes of morbidity and mortality worldwide	127:181	Atherosclerotic cardiovascular diseases remain the leading causes of morbidity and mortality worldwide.
35870569	10	75	from	inflammation	1906:1917	arg1	plaques					1952:1958	atherosclerotic plaques	1936:1958	atherosclerotic plaques	1936:1958	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	4	76	theme	solubilizing	503:514	arg1	cholesterol					528:538	solubilizing hydrophobic cholesterol	503:538	solubilizing hydrophobic cholesterol	503:538	Previous studies identified the hydrophobic domain of lipid bilayer in liposomes acted as sinks for solubilizing hydrophobic cholesterol.
35870569	10	77	theme	atherosclerotic	1936:1950	arg1	plaques					1952:1958	atherosclerotic plaques	1936:1958	atherosclerotic plaques	1936:1958	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	11	78	theme	cholesterol	2051:2061	arg1	crystals					2063:2070	cholesterol crystals	2051:2070	cholesterol crystals in atherosclerotic plaques	2051:2097	Therefore, AnxV-Rb1-LPs provide a potential strategy for removing cholesterol crystals in atherosclerotic plaques and can be further utilized in other diseases with excessive cholesterol accumulation.
35870569	8	79	theme	bilayer	1393:1399	arg1	solubilization					1429:1442	the solubilization	1425:1442	the solubilization of cholesterol crystals	1425:1466	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	8	79	theme	bilayer	1393:1399	arg1	affinity					1371:1378	the affinity	1367:1378	the affinity of the lipid bilayer to free cholesterol	1367:1419	The in vitro studies showed that incorporating Rb1 into lipid bilayer remarkably increased the affinity of the lipid bilayer to free cholesterol and the solubilization of cholesterol crystals.
35870569	10	80	theme	extracellular	1847:1859	arg1	crystals					1873:1880	intra- and extracellular cholesterol crystals	1836:1880	intra- and extracellular cholesterol crystals	1836:1880	Furthermore, AnxV-Rb1-LPs could efficiently accumulate in atherosclerotic plaques after intravenous injection, exert nano-sponge-like functions to remove intra- and extracellular cholesterol crystals, ultimately alleviating inflammation and apoptosis in atherosclerotic plaques for antiatherosclerosis.
35870569	2	81	theme	essential	240:248	arg1	role					250:253	an essential role	237:253	an essential role	237:253	Cholesterol crystals in atherosclerotic plaques play an essential role in atherosclerosis progression.
35870569	6	82	theme	lipid	978:982	arg1	bilayer					984:990	lipid bilayer	978:990	lipid bilayer	978:990	Therefore, in this study, ginsenosides Rb1 (Rb1), one of main active components of ginseng which has a similar structure to cholesterol, is anchored into soy phospholipids bilayer with its hydrophobic region to prepare nano-sponge-like liposomes (Rb1-LPs), aiming to amplify the solubilization of cholesterol in lipid bilayer.
35080234	2	0	theme	trehalose	441:449	arg1	deterioration					455:467	deterioration	455:467	deterioration of cells	455:476	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	2	0	theme	trehalose	441:449	arg1	effect					431:436	the cryoprotective effect	412:436	the cryoprotective effect of trehalose	412:449	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	5	1	theme	membrane	1054:1061	arg1	perturbation					1063:1074	membrane perturbation	1054:1074	membrane perturbation with protection of PVP	1054:1097	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	6	2	contain	containing	1339:1348	arg1	those					1333:1337	those	1333:1337	those	1333:1337	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	6	2	contain	containing	1339:1348	arg2	trehalose					1350:1358	trehalose	1350:1358	trehalose only	1350:1363	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	6	3	theme	6.1	1244:1246	arg1	±					1242:1242	±	1242:1242	±	1242:1242	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	1	4	theme	time-consuming	209:222	arg1	essential					268:276	essential	268:276	essential	268:276	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	4	theme	time-consuming	209:222	arg1	processes					254:262	the time-consuming thawing and deglycerolization processes	205:262	the time-consuming thawing and deglycerolization processes	205:262	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	3	5	theme	cryopreservation	640:655	arg1	system					657:662	a novel cryopreservation system	632:662	a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4	632:722	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	1	6	theme	conventional	139:150	arg1	cryoprotectant					152:165	a conventional cryoprotectant	137:165	a conventional cryoprotectant of human red blood cells (hRBCs)	137:198	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	6	theme	conventional	139:150	arg1	glycerol					125:132	glycerol	125:132	glycerol	125:132	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	7	theme	thawing	224:230	arg1	essential					268:276	essential	268:276	essential	268:276	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	7	theme	thawing	224:230	arg1	processes					254:262	the time-consuming thawing and deglycerolization processes	205:262	the time-consuming thawing and deglycerolization processes	205:262	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	6	8	theme	±	1299:1299	arg1	%					1304:1304	91.7 ± 5.5%	1294:1304	91.7 ± 5.5%	1294:1304	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	2	9	from	°C	404:405	arg1	hRBCs					392:396	hRBCs	392:396	hRBCs at 37 °C	392:405	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	7	10	theme	biocompatible	1436:1448	arg1	system					1468:1473	the biocompatible trehalose-loading system	1432:1473	the biocompatible trehalose-loading system	1432:1473	It was observed that the biocompatible trehalose-loading system could benefit glycerol-free cryopreservation of hRBCs and also provide a feasible way for impermeable biomacromolecule delivery.
35080234	6	11	theme	91.7	1294:1297	arg1	%					1304:1304	91.7 ± 5.5%	1294:1304	91.7 ± 5.5%	1294:1304	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	6	12	dep	±	1371:1371	arg1	mM					1377:1378	1.1 mM	1373:1378	11.9 ± 1.1 mM	1366:1378	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	2	13	dep	now	325:327	arg1	up					319:320	up	319:320	up	319:320	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	1	14	theme	deglycerolization	236:252	arg1	essential					268:276	essential	268:276	essential	268:276	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	14	theme	deglycerolization	236:252	arg1	processes					254:262	the time-consuming thawing and deglycerolization processes	205:262	the time-consuming thawing and deglycerolization processes	205:262	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	2	15	theme	cells	472:476	arg1	deterioration					455:467	deterioration	455:467	deterioration of cells	455:476	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	2	15	theme	cells	472:476	arg1	effect					431:436	the cryoprotective effect	412:436	the cryoprotective effect of trehalose	412:449	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	6	16	theme	intracellular	1191:1203	arg1	trehalose					1205:1213	the intracellular trehalose	1187:1213	the intracellular trehalose	1187:1213	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	4	17	theme	High	725:728	arg1	contents					754:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	5	18	from	effect	968:973	arg1	perturbation					1063:1074	membrane perturbation	1054:1074	membrane perturbation with protection of PVP	1054:1097	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	7	19	theme	trehalose-loading	1450:1466	arg1	system					1468:1473	the biocompatible trehalose-loading system	1432:1473	the biocompatible trehalose-loading system	1432:1473	It was observed that the biocompatible trehalose-loading system could benefit glycerol-free cryopreservation of hRBCs and also provide a feasible way for impermeable biomacromolecule delivery.
35080234	3	20	theme	trehalose	678:686	arg1	entry					688:692	trehalose entry	678:692	trehalose entry into hRBCs	678:703	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	0	21	theme	glycerol-free	82:94	arg1	cryopreservation					96:111	glycerol-free cryopreservation	82:111	glycerol-free cryopreservation	82:111	Facilitating trehalose entry into hRBCs at 4 °C by alkylated ε-poly(L-lysine) for glycerol-free cryopreservation.
35080234	0	22	theme	trehalose	13:21	arg1	entry					23:27	trehalose entry	13:27	trehalose entry into hRBCs	13:38	Facilitating trehalose entry into hRBCs at 4 °C by alkylated ε-poly(L-lysine) for glycerol-free cryopreservation.
35080234	4	23	theme	function	814:821	arg1	variations					823:832	small function variations	808:832	small function variations	808:832	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	1	24	theme	human	170:174	arg1	cells					186:190	human red blood cells	170:190	human red blood cells (hRBCs)	170:198	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	24	theme	human	170:174	arg1	hRBCs					193:197	hRBCs	193:197	hRBCs	193:197	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	4	25	theme	intracellular	730:742	arg1	contents					754:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	4	26	theme	small	808:812	arg1	variations					823:832	small function variations	808:832	small function variations	808:832	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	3	27	theme	hydrophobic	537:547	arg1	groups					561:566	hydrophobic or cationic groups	537:566	hydrophobic or cationic groups	537:566	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	1	28	theme	red	176:178	arg1	cells					186:190	human red blood cells	170:190	human red blood cells (hRBCs)	170:198	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	28	theme	red	176:178	arg1	hRBCs					193:197	hRBCs	193:197	hRBCs	193:197	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	4	29	theme	ε-poly	898:903	arg1	nanoparticles					871:883	self-assembled nanoparticles	856:883	self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP)	856:961	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	4	30	theme	hRBCs	783:787	arg1	contents					754:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	4	30	theme	hRBCs	783:787	arg1	cryosurvival					767:778	cryosurvival	767:778	cryosurvival of hRBCs	767:787	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	1	31	theme	blood	180:184	arg1	cells					186:190	human red blood cells	170:190	human red blood cells (hRBCs)	170:198	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	31	theme	blood	180:184	arg1	hRBCs					193:197	hRBCs	193:197	hRBCs	193:197	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	4	32	theme	alkylated	888:896	arg1	ε-PL					916:919	ε-PL	916:919	ε-PL	916:919	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	4	32	theme	alkylated	888:896	arg1	ε-poly					898:903	alkylated ε-poly	888:903	alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP)	888:961	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	4	32	theme	alkylated	888:896	arg1	L-lysine					905:912	L-lysine	905:912	L-lysine	905:912	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	5	33	theme	ε-PL	1000:1003	arg1	effect					968:973	The effect	964:973	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP	964:1097	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	5	34	with	ε-PL	1000:1003	arg1	chains					1024:1029	various alkyl chains	1010:1029	various alkyl chains	1010:1029	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	5	34	with	ε-PL	1000:1003	arg1	ratios					1044:1049	grafting ratios	1035:1049	grafting ratios	1035:1049	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	1	35	theme	cells	186:190	arg1	cryoprotectant					152:165	a conventional cryoprotectant	137:165	a conventional cryoprotectant of human red blood cells (hRBCs)	137:198	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	1	35	theme	cells	186:190	arg1	glycerol					125:132	glycerol	125:132	glycerol	125:132	Currently, glycerol is a conventional cryoprotectant of human red blood cells (hRBCs), but the time-consuming thawing and deglycerolization processes are essential before transfusion.
35080234	7	36	theme	feasible	1548:1555	arg1	way					1557:1559	a feasible way	1546:1559	a feasible way for impermeable biomacromolecule delivery	1546:1601	It was observed that the biocompatible trehalose-loading system could benefit glycerol-free cryopreservation of hRBCs and also provide a feasible way for impermeable biomacromolecule delivery.
35080234	3	37	theme	groups	561:566	arg1	interaction					522:532	the interaction	518:532	the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization	518:610	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	7	38	theme	biomacromolecule	1577:1592	arg1	delivery					1594:1601	impermeable biomacromolecule delivery	1565:1601	impermeable biomacromolecule delivery	1565:1601	It was observed that the biocompatible trehalose-loading system could benefit glycerol-free cryopreservation of hRBCs and also provide a feasible way for impermeable biomacromolecule delivery.
35080234	2	39	theme	trehalose	379:387	arg1	delivery					355:362	the delivery	351:362	the delivery of impermeable trehalose to hRBCs at 37 °C	351:405	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	2	40	theme	cryoprotective	416:429	arg1	effect					431:436	the cryoprotective effect	412:436	the cryoprotective effect of trehalose	412:449	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	7	41	theme	hRBCs	1523:1527	arg1	cryopreservation					1503:1518	glycerol-free cryopreservation	1489:1518	glycerol-free cryopreservation of hRBCs	1489:1527	It was observed that the biocompatible trehalose-loading system could benefit glycerol-free cryopreservation of hRBCs and also provide a feasible way for impermeable biomacromolecule delivery.
35080234	4	42	theme	vinyl	938:942	arg1	poly					933:936	poly	933:936	poly(vinyl pyrrolidone) (PVP)	933:961	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	4	42	theme	vinyl	938:942	arg1	pyrrolidone					944:954	vinyl pyrrolidone	938:954	vinyl pyrrolidone	938:954	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	5	43	theme	various	1010:1016	arg1	chains					1024:1029	various alkyl chains	1010:1029	various alkyl chains	1010:1029	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	6	44	theme	ε-PL	1173:1176	arg1	combination					1148:1158	the combination	1144:1158	the combination of alkylated ε-PL and PVP	1144:1184	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	2	45	theme	impermeable	367:377	arg1	trehalose					379:387	impermeable trehalose	367:387	impermeable trehalose	367:387	Much of the research up to now has been conducted on the delivery of impermeable trehalose to hRBCs at 37 °C, but the cryoprotective effect of trehalose and deterioration of cells still remain challenging.
35080234	7	46	theme	impermeable	1565:1575	arg1	delivery					1594:1601	impermeable biomacromolecule delivery	1565:1601	impermeable biomacromolecule delivery	1565:1601	It was observed that the biocompatible trehalose-loading system could benefit glycerol-free cryopreservation of hRBCs and also provide a feasible way for impermeable biomacromolecule delivery.
35080234	4	47	theme	self-assembled	856:869	arg1	nanoparticles					871:883	self-assembled nanoparticles	856:883	self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP)	856:961	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	5	48	theme	alkyl	1018:1022	arg1	chains					1024:1029	various alkyl chains	1010:1029	various alkyl chains	1010:1029	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	6	49	theme	alkylated	1163:1171	arg1	ε-PL					1173:1176	alkylated ε-PL	1163:1176	alkylated ε-PL	1163:1176	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	5	50	theme	grafting	1035:1042	arg1	ratios					1044:1049	grafting ratios	1035:1049	grafting ratios	1035:1049	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	5	51	theme	PVP	1095:1097	arg1	protection					1081:1090	protection	1081:1090	protection of PVP	1081:1097	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	6	52	theme	hRBC	1268:1271	arg1	cryosurvival					1273:1284	hRBC cryosurvival	1268:1284	hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively	1268:1408	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	3	53	theme	cationic	552:559	arg1	groups					561:566	hydrophobic or cationic groups	537:566	hydrophobic or cationic groups	537:566	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	6	54	theme	109.7	1236:1240	arg1	±					1242:1242	±	1242:1242	±	1242:1242	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	3	55	theme	novel	634:638	arg1	system					657:662	a novel cryopreservation system	632:662	a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4	632:722	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	4	56	theme	trehalose	744:752	arg1	contents					754:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents	725:761	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	3	57	from	interaction	522:532	arg1	membranes					576:584	cell membranes	571:584	cell membranes	571:584	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	3	57	from	interaction	522:532	arg1	stabilization					598:610	osmotic stabilization	590:610	osmotic stabilization	590:610	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	4	58	theme	nanoparticles	871:883	arg1	assistance					842:851	the assistance	838:851	the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP)	838:961	High intracellular trehalose contents and cryosurvival of hRBCs were achieved with small function variations via the assistance of self-assembled nanoparticles of alkylated ε-poly(L-lysine) (ε-PL) along with poly(vinyl pyrrolidone) (PVP).
35080234	5	59	theme	amphipathic	978:988	arg1	ε-PL					1000:1003	amphipathic alkylated ε-PL	978:1003	amphipathic alkylated ε-PL with various alkyl chains and grafting ratios	978:1049	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	3	60	theme	osmotic	590:596	arg1	stabilization					598:610	osmotic stabilization	590:610	osmotic stabilization	590:610	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	6	61	theme	±	1389:1389	arg1	%					1394:1394	50.0 ± 2.1%	1384:1394	50.0 ± 2.1%	1384:1394	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	0	62	theme	alkylated	51:59	arg1	ε-poly					61:66	alkylated ε-poly	51:66	alkylated ε-poly(L-lysine)	51:76	Facilitating trehalose entry into hRBCs at 4 °C by alkylated ε-poly(L-lysine) for glycerol-free cryopreservation.
35080234	0	62	theme	alkylated	51:59	arg1	L-lysine					68:75	L-lysine	68:75	L-lysine	68:75	Facilitating trehalose entry into hRBCs at 4 °C by alkylated ε-poly(L-lysine) for glycerol-free cryopreservation.
35080234	5	63	theme	alkylated	990:998	arg1	ε-PL					1000:1003	amphipathic alkylated ε-PL	978:1003	amphipathic alkylated ε-PL with various alkyl chains and grafting ratios	978:1049	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	3	64	theme	cell	571:574	arg1	membranes					576:584	cell membranes	571:584	cell membranes	571:584	Encouraged by the interaction of hydrophobic or cationic groups on cell membranes and osmotic stabilization, herein, we propose a novel cryopreservation system to facilitate trehalose entry into hRBCs at 4 °C and pH 7.4.
35080234	6	65	theme	PVP	1182:1184	arg1	combination					1148:1158	the combination	1144:1158	the combination of alkylated ε-PL and PVP	1144:1184	Overall, by the combination of alkylated ε-PL and PVP, the intracellular trehalose could be enhanced to 109.7 ± 6.1 mM and subsequently hRBC cryosurvival reached 91.7 ± 5.5%, significantly higher than those containing trehalose only, 11.9 ± 1.1 mM and 50.0 ± 2.1%, respectively.
35080234	5	66	with	perturbation	1063:1074	arg1	protection					1081:1090	protection	1081:1090	protection of PVP	1081:1097	The effect of amphipathic alkylated ε-PL with various alkyl chains and grafting ratios on membrane perturbation with protection of PVP was systematically investigated.
35080234	7	67	theme	glycerol-free	1489:1501	arg1	cryopreservation					1503:1518	glycerol-free cryopreservation	1489:1518	glycerol-free cryopreservation of hRBCs	1489:1527	It was observed that the biocompatible trehalose-loading system could benefit glycerol-free cryopreservation of hRBCs and also provide a feasible way for impermeable biomacromolecule delivery.
36012231	0	0	theme	Pectin-Based	83:94	arg1	Films					96:100	Biodegradable Pectin-Based Films	69:100	Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring	69:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	9	1	theme	food	1525:1528	arg1	shelf-life					1530:1539	food shelf-life	1525:1539	food shelf-life	1525:1539	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	2	theme	films	1415:1419	arg1	development					1387:1397	the development	1383:1397	the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1383:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	5	3	theme	different	718:726	arg1	berries					728:734	different berries	718:734	different berries	718:734	Then, pectin films with the extracts of different berries were developed and characterized.
36012231	0	4	theme	Biodegradable	69:81	arg1	Films					96:100	Biodegradable Pectin-Based Films	69:100	Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring	69:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	6	5	theme	=	856:856	arg1	*					854:854	L* = 61.42	853:862	L* = 61.42	853:862	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	9	6	theme	shelf-life	1530:1539	arg1	extension					1512:1520	the extension	1508:1520	the extension of food shelf-life	1508:1539	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	6	7	theme	blueberry	798:806	arg1	thicker					825:831	thicker	825:831	thicker	825:831	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	6	7	theme	blueberry	798:806	arg1	film					816:819	the blueberry extract film	794:819	the blueberry extract film	794:819	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	3	8	theme	salmon	483:488	arg1	shelf-life					490:499	salmon shelf-life	483:499	salmon shelf-life	483:499	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	6	9	theme	highest	898:904	arg1	density					906:912	the highest density	894:912	the highest density (1.477 g/cm3)	894:926	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	6	9	theme	highest	898:904	arg1	g/cm3					921:925	1.477 g/cm3	915:925	1.477 g/cm3	915:925	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	0	10	from	Valorization	0:11	arg1	Development					54:64	the Development	50:64	the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring	50:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	6	11	theme	L	853:853	arg1	*					854:854	L* = 61.42	853:862	L* = 61.42	853:862	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	0	12	theme	Films	96:100	arg1	Development					54:64	the Development	50:64	the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring	50:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	4	13	theme	flavonols	606:614	arg1	terms					597:601	terms	597:601	terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity	597:675	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	8	14	theme	pH	1216:1217	arg1	deterioration					1223:1235	deterioration	1223:1235	deterioration of fish	1223:1243	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	8	14	theme	pH	1216:1217	arg1	values					1206:1211	lower values	1200:1211	lower values of pH	1200:1217	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	8	15	theme	blueberry	1177:1185	arg1	films					1187:1191	blueberry films	1177:1191	blueberry films	1177:1191	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	0	16	theme	Salmon	112:117	arg1	Monitoring					144:153	Fresh Salmon (Salmo salar) Shelf-Life Monitoring	106:153	Fresh Salmon (Salmo salar) Shelf-Life Monitoring	106:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	9	17	theme	sustainable	1546:1556	arg1	alternative					1568:1578	a sustainable packaging alternative	1544:1578	a sustainable packaging alternative	1544:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	2	18	used	used	341:344	arg2	wastes					314:319	these wastes	308:319	these wastes	308:319	Nonetheless, these wastes can be revalued and used.
36012231	1	19	theme	healthy	160:166	arg1	properties					168:177	The healthy properties	156:177	The healthy properties of berries	156:188	The healthy properties of berries are known; however, red fruits are very perishable, generating large losses in production and marketing.
36012231	0	20	theme	Fresh	106:110	arg1	salar					126:130	Salmo salar	120:130	Salmo salar	120:130	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	0	20	theme	Fresh	106:110	arg1	Salmon					112:117	Fresh Salmon	106:117	Fresh Salmon (Salmo salar) Shelf-Life Monitoring	106:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	7	21	from	composition	1054:1064	arg1	compounds					1079:1087	bioactive compounds	1069:1087	bioactive compounds	1069:1087	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	9	22	theme	packaging	1558:1566	arg1	alternative					1568:1578	a sustainable packaging alternative	1544:1578	a sustainable packaging alternative	1544:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	3	23	theme	biodegradable	403:415	arg1	films					424:428	biodegradable pectin films	403:428	biodegradable pectin films with berry agro-industrial waste extracts	403:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	3	24	theme	main	351:354	arg1	objective					356:364	The main objective	347:364	The main objective of this study	347:378	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	3	24	theme	main	351:354	arg1	development					388:398	the development	384:398	the development of biodegradable pectin films with berry agro-industrial waste extracts	384:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	4	25	dep	blueberries	529:539	arg1	wastes					572:577	wastes	572:577	wastes	572:577	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	9	26	used	used	1433:1436	arg2	films					1415:1419	eco-friendly films	1402:1419	eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1402:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	26	used	used	1433:1436	arg2	materials					1482:1490	intelligent food packaging materials	1455:1490	intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1455:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	27	theme	food	1467:1470	arg1	materials					1482:1490	intelligent food packaging materials	1455:1490	intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1455:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	27	theme	food	1467:1470	arg1	films					1415:1419	eco-friendly films	1402:1419	eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1402:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	4	28	from	blackberries	542:553	arg1	extracts					515:522	The obtained extracts	502:522	The obtained extracts from blueberries, blackberries, and raspberries wastes	502:577	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	1	29	theme	large	253:257	arg1	losses					259:264	large losses	253:264	large losses in production and marketing	253:292	The healthy properties of berries are known; however, red fruits are very perishable, generating large losses in production and marketing.
36012231	1	30	from	losses	259:264	arg1	marketing					284:292	marketing	284:292	marketing	284:292	The healthy properties of berries are known; however, red fruits are very perishable, generating large losses in production and marketing.
36012231	1	30	from	losses	259:264	arg1	production					269:278	production	269:278	production	269:278	The healthy properties of berries are known; however, red fruits are very perishable, generating large losses in production and marketing.
36012231	8	31	theme	fish	1240:1243	arg1	deterioration					1223:1235	deterioration	1223:1235	deterioration of fish	1223:1243	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	8	31	theme	fish	1240:1243	arg1	values					1206:1211	lower values	1200:1211	lower values of pH	1200:1217	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	1	32	theme	berries	182:188	arg1	properties					168:177	The healthy properties	156:177	The healthy properties of berries	156:188	The healthy properties of berries are known; however, red fruits are very perishable, generating large losses in production and marketing.
36012231	9	33	theme	agro-industrial	1358:1372	arg1	waste					1374:1378	berries agro-industrial waste	1350:1378	berries agro-industrial waste	1350:1378	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	0	34	theme	Salmo	120:124	arg1	salar					126:130	Salmo salar	120:130	Salmo salar	120:130	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	0	34	theme	Salmo	120:124	arg1	Salmon					112:117	Fresh Salmon	106:117	Fresh Salmon (Salmo salar) Shelf-Life Monitoring	106:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	7	35	from	pHs	1142:1144	arg1	application					1029:1039	further application	1021:1039	further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs	1021:1144	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	7	36	from	change	1122:1127	arg1	compounds					1079:1087	bioactive compounds	1069:1087	bioactive compounds	1069:1087	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	5	37	with	films	691:695	arg1	extracts					706:713	the extracts	702:713	the extracts of different berries	702:734	Then, pectin films with the extracts of different berries were developed and characterized.
36012231	4	38	from	blueberries	529:539	arg1	extracts					515:522	The obtained extracts	502:522	The obtained extracts from blueberries, blackberries, and raspberries wastes	502:577	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	6	39	dep	thicker	825:831	arg1	mm					840:841	0.248 mm	834:841	0.248 mm	834:841	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	3	40	with	films	424:428	arg1	extracts					463:470	berry agro-industrial waste extracts	435:470	berry agro-industrial waste extracts	435:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	9	41	theme	intelligent	1455:1465	arg1	materials					1482:1490	intelligent food packaging materials	1455:1490	intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1455:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	41	theme	intelligent	1455:1465	arg1	films					1415:1419	eco-friendly films	1402:1419	eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1402:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	3	42	theme	berry	435:439	arg1	extracts					463:470	berry agro-industrial waste extracts	435:470	berry agro-industrial waste extracts	435:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	3	43	theme	study	374:378	arg1	objective					356:364	The main objective	347:364	The main objective of this study	347:378	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	3	43	theme	study	374:378	arg1	development					388:398	the development	384:398	the development of biodegradable pectin films with berry agro-industrial waste extracts	384:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	7	44	theme	different	1132:1140	arg1	pHs					1142:1144	different pHs	1132:1144	different pHs	1132:1144	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	8	45	theme	lower	1200:1204	arg1	values					1206:1211	lower values	1200:1211	lower values of pH	1200:1217	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	6	46	theme	raspberry	945:953	arg1	films					955:959	the raspberry films	941:959	the raspberry films	941:959	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	3	47	theme	pectin	417:422	arg1	films					424:428	biodegradable pectin films	403:428	biodegradable pectin films with berry agro-industrial waste extracts	403:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	0	48	theme	Agro-Industrial	25:39	arg1	Waste					41:45	Berries' Agro-Industrial Waste	16:45	Berries' Agro-Industrial Waste	16:45	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	4	49	theme	obtained	506:513	arg1	extracts					515:522	The obtained extracts	502:522	The obtained extracts from blueberries, blackberries, and raspberries wastes	502:577	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	6	50	dep	darker	845:850	arg1	*					854:854	L* = 61.42	853:862	L* = 61.42	853:862	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	6	51	theme	0.248	834:838	arg1	mm					840:841	0.248 mm	834:841	0.248 mm	834:841	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	4	52	dep	phenols	617:623	arg1	contents					642:649	contents	642:649	contents	642:649	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	3	53	theme	films	424:428	arg1	objective					356:364	The main objective	347:364	The main objective of this study	347:378	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	3	53	theme	films	424:428	arg1	development					388:398	the development	384:398	the development of biodegradable pectin films with berry agro-industrial waste extracts	384:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	9	54	theme	eco-friendly	1402:1413	arg1	materials					1482:1490	intelligent food packaging materials	1455:1490	intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1455:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	54	theme	eco-friendly	1402:1413	arg1	films					1415:1419	eco-friendly films	1402:1419	eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1402:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	6	55	dep	opaquer	870:876	arg1	%					884:884	17.71%	879:884	17.71%	879:884	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	9	56	theme	berries	1350:1356	arg1	waste					1374:1378	berries agro-industrial waste	1350:1378	berries agro-industrial waste	1350:1378	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	57	theme	waste	1374:1378	arg1	valorization					1334:1345	the valorization	1330:1345	the valorization of berries agro-industrial waste	1330:1378	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	7	58	theme	further	1021:1027	arg1	application					1029:1039	further application	1021:1039	further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs	1021:1144	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	0	59	theme	Shelf-Life	133:142	arg1	Monitoring					144:153	Fresh Salmon (Salmo salar) Shelf-Life Monitoring	106:153	Fresh Salmon (Salmo salar) Shelf-Life Monitoring	106:153	Valorization of Berries' Agro-Industrial Waste in the Development of Biodegradable Pectin-Based Films for Fresh Salmon (Salmo salar) Shelf-Life Monitoring.
36012231	7	60	from	capacity	1102:1109	arg1	compounds					1079:1087	bioactive compounds	1069:1087	bioactive compounds	1069:1087	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	8	61	theme	control	1276:1282	arg1	samples					1295:1301	the control and pectin samples	1272:1301	samples	1295:1301	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	9	62	theme	packaging	1472:1480	arg1	materials					1482:1490	intelligent food packaging materials	1455:1490	intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1455:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	9	62	theme	packaging	1472:1480	arg1	films					1415:1419	eco-friendly films	1402:1419	eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative	1402:1578	This study contributes to the valorization of berries agro-industrial waste by the development of eco-friendly films that can be used in the future as intelligent food packaging materials contributing to the extension of food shelf-life as a sustainable packaging alternative.
36012231	3	63	theme	agro-industrial	441:455	arg1	extracts					463:470	berry agro-industrial waste extracts	435:470	berry agro-industrial waste extracts	435:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	4	64	from	raspberries	560:570	arg1	extracts					515:522	The obtained extracts	502:522	The obtained extracts from blueberries, blackberries, and raspberries wastes	502:577	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	8	65	theme	pectin	1288:1293	arg1	samples					1295:1301	the control and pectin samples	1272:1301	samples	1295:1301	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	3	66	theme	waste	457:461	arg1	extracts					463:470	berry agro-industrial waste extracts	435:470	berry agro-industrial waste extracts	435:470	The main objective of this study was the development of biodegradable pectin films with berry agro-industrial waste extracts to monitor salmon shelf-life.
36012231	5	67	theme	pectin	684:689	arg1	films					691:695	pectin films	684:695	pectin films with the extracts of different berries	684:734	Then, pectin films with the extracts of different berries were developed and characterized.
36012231	1	68	theme	red	210:212	arg1	fruits					214:219	red fruits	210:219	red fruits	210:219	The healthy properties of berries are known; however, red fruits are very perishable, generating large losses in production and marketing.
36012231	5	69	theme	berries	728:734	arg1	extracts					706:713	the extracts	702:713	the extracts of different berries	702:734	Then, pectin films with the extracts of different berries were developed and characterized.
36012231	4	70	theme	phenols	617:623	arg1	terms					597:601	terms	597:601	terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity	597:675	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	8	71	theme	salmon	1151:1156	arg1	samples					1158:1164	The salmon samples	1147:1164	The salmon samples wrapped in blueberry films	1147:1191	The salmon samples wrapped in blueberry films showed lower values of pH and deterioration of fish during storage compared to the control and pectin samples.
36012231	4	72	theme	capacity	668:675	arg1	terms					597:601	terms	597:601	terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity	597:675	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	6	73	theme	extract	808:814	arg1	thicker					825:831	thicker	825:831	thicker	825:831	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	6	73	theme	extract	808:814	arg1	film					816:819	the blueberry extract film	794:819	the blueberry extract film	794:819	The results showed that the blueberry extract film was thicker (0.248 mm), darker (L* = 61.42), and opaquer (17.71%), while the highest density (1.477 g/cm3) was shown by the raspberry films.
36012231	7	74	theme	color	1116:1120	arg1	change					1122:1127	color change	1116:1127	color change	1116:1127	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	7	75	theme	bioactive	1069:1077	arg1	compounds					1079:1087	bioactive compounds	1069:1087	bioactive compounds	1069:1087	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
36012231	4	76	theme	antioxidant	656:666	arg1	capacity					668:675	antioxidant capacity	656:675	antioxidant capacity	656:675	The obtained extracts from blueberries, blackberries, and raspberries wastes were evaluated in terms of flavonols, phenols and anthocyanins contents, and antioxidant capacity.
36012231	7	77	theme	antioxidant	1090:1100	arg1	capacity					1102:1109	antioxidant capacity	1090:1109	antioxidant capacity	1090:1109	The results also showed that blueberries were the best for further application due to their composition in bioactive compounds, antioxidant capacity, and color change at different pHs.
35022427	9	0	from	expression	1155:1164	arg1	spleen					1173:1178	the spleen	1169:1178	the spleen	1169:1178	The diet with increased dietary fat increased Il1b but decreased Cd8a gene expression in the spleen.
35022427	8	1	theme	increased	1065:1073	arg1	fat					1075:1077	increased fat	1065:1077	increased fat	1065:1077	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	13	2	theme	Immulix	1554:1560	arg1	feeding					1562:1568	Immulix feeding	1554:1568	Immulix feeding	1554:1568	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	2	theme	Immulix	1554:1560	arg1	cytokines					1529:1537	cytokines	1529:1537	cytokines	1529:1537	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	3	from	influences	1494:1503	arg1	markers					1520:1526	immune cell markers	1508:1526	immune cell markers	1508:1526	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	4	4	theme	tetanus	588:594	arg1	vaccine					596:602	a tetanus vaccine	586:602	a tetanus vaccine in 48 BALB/cJTac mice	586:624	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	9	5	theme	dietary	1104:1110	arg1	fat					1112:1114	increased dietary fat	1094:1114	increased dietary fat	1094:1114	The diet with increased dietary fat increased Il1b but decreased Cd8a gene expression in the spleen.
35022427	11	6	theme	fat	1255:1257	arg1	content					1259:1265	Increased dietary fat content	1237:1265	Increased dietary fat content	1237:1265	Increased dietary fat content upregulated Lactobacillus animalis but downregulated an unclassified Prevotella spp.
35022427	2	7	theme	young	287:291	arg1	individuals					293:303	young individuals	287:303	young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM)	287:395	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	4	8	theme	antibody	567:574	arg1	titers					576:581	antibody titers	567:581	antibody titers	567:581	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	11	9	theme	Increased	1237:1245	arg1	content					1259:1265	Increased dietary fat content	1237:1265	Increased dietary fat content	1237:1265	Increased dietary fat content upregulated Lactobacillus animalis but downregulated an unclassified Prevotella spp.
35022427	13	10	theme	IgG	1543:1545	arg1	titers					1547:1552	IgG titers	1543:1552	IgG titers	1543:1552	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	5	11	theme	Female	649:654	arg1	mice					656:659	Female mice	649:659	Female mice	649:659	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	9	12	theme	Cd8a	1145:1148	arg1	expression					1155:1164	Cd8a gene expression	1145:1164	Cd8a gene expression in the spleen	1145:1178	The diet with increased dietary fat increased Il1b but decreased Cd8a gene expression in the spleen.
35022427	13	13	theme	immune	1508:1513	arg1	markers					1520:1526	immune cell markers	1508:1526	immune cell markers	1508:1526	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	1	14	theme	mammalian	104:112	arg1	species					114:120	Several mammalian species	96:120	Several mammalian species	96:120	Several mammalian species are vaccinated in early life, but little is known about the effect of diet on vaccine response.
35022427	0	15	from	responses	77:85	arg1	mice					90:93	mice	90:93	mice	90:93	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	4	16	theme	BALB/cJTac	610:619	arg1	mice					621:624	48 BALB/cJTac mice	607:624	48 BALB/cJTac mice	607:624	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	0	17	theme	minor	53:57	arg1	impact					59:64	minor impact	53:64	minor impact	53:64	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	7	18	theme	Il1b	913:916	arg1	expression					923:932	Il1b gene expression	913:932	Il1b gene expression	913:932	Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen.
35022427	13	19	theme	tetanus	1653:1659	arg1	responses					1669:1677	tetanus vaccine responses	1653:1677	tetanus vaccine responses in this experiment	1653:1696	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	5	20	theme	higher	702:707	arg1	variation					709:717	higher variation	702:717	higher variation	702:717	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	8	21	theme	downregulating	963:976	arg1	effect					978:983	a downregulating effect	961:983	a downregulating effect on expression of Cd4 and Foxp3 in ileum	961:1023	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	7	22	theme	secondary	875:883	arg1	vaccination					885:895	secondary vaccination	875:895	secondary vaccination	875:895	Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen.
35022427	5	23	theme	higher	679:684	arg1	titers					690:695	significantly higher IgG titers	665:695	significantly higher IgG titers with higher variation	665:717	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	2	24	theme	gut	377:379	arg1	microbiota					381:390	gut microbiota	377:390	gut microbiota (GM)	377:395	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	2	24	theme	gut	377:379	arg1	GM					393:394	GM	393:394	GM	393:394	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	0	25	theme	Oligosaccharide	0:14	arg1	Immulix					40:46	Immulix	40:46	Immulix	40:46	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	0	25	theme	Oligosaccharide	0:14	arg1	supplement					28:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	13	26	from	effects	1642:1648	arg1	mice					1701:1704	mice	1701:1704	mice	1701:1704	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	26	from	effects	1642:1648	arg1	responses					1669:1677	tetanus vaccine responses	1653:1677	tetanus vaccine responses in this experiment	1653:1696	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	0	27	theme	feed	23:26	arg1	Immulix					40:46	Immulix	40:46	Immulix	40:46	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	0	27	theme	feed	23:26	arg1	supplement					28:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	4	28	theme	oligosaccharide	490:504	arg1	Immulix					512:518	Immulix	512:518	Immulix	512:518	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	4	28	theme	oligosaccharide	490:504	arg1	diet					506:509	an oligosaccharide diet	487:509	an oligosaccharide diet (Immulix)	487:519	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	8	29	contain	had	957:959	arg2	effect					978:983	a downregulating effect	961:983	a downregulating effect on expression of Cd4 and Foxp3 in ileum	961:1023	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	8	29	contain	had	957:959	arg1	Immulix					949:955	Immulix	949:955	Immulix	949:955	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	13	30	from	responses	1669:1677	arg1	experiment					1687:1696	this experiment	1682:1696	this experiment	1682:1696	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	6	31	theme	content	786:792	arg1	minor					799:803	minor	799:803	minor	799:803	The effects of Immulix and/or increased fat content were minor.
35022427	6	31	theme	content	786:792	arg1	effects					746:752	The effects	742:752	The effects of Immulix and/or increased fat content	742:792	The effects of Immulix and/or increased fat content were minor.
35022427	13	32	theme	increased	1573:1581	arg1	content					1595:1601	increased dietary fat content	1573:1601	increased dietary fat content	1573:1601	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	32	theme	increased	1573:1581	arg1	cytokines					1529:1537	cytokines	1529:1537	cytokines	1529:1537	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	2	33	theme	anti-inflammatory	318:334	arg1	potential					336:344	their anti-inflammatory potential	312:344	their anti-inflammatory potential elicited through modulation of gut microbiota (GM)	312:395	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	5	34	theme	male	731:734	arg1	mice					736:739	male mice	731:739	male mice	731:739	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	6	35	theme	increased	772:780	arg1	content					786:792	increased fat content	772:792	increased fat content	772:792	The effects of Immulix and/or increased fat content were minor.
35022427	13	36	theme	fat	1591:1593	arg1	content					1595:1601	increased dietary fat content	1573:1601	increased dietary fat content	1573:1601	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	36	theme	fat	1591:1593	arg1	cytokines					1529:1537	cytokines	1529:1537	cytokines	1529:1537	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	3	37	theme	fat	431:433	arg1	fraction					435:442	the fat fraction	427:442	the fat fraction	427:442	Also, diet, e.g. the size of the fat fraction, is known to modulate the GM.
35022427	7	38	theme	male	848:851	arg1	mice					853:856	male mice	848:856	male mice	848:856	Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen.
35022427	6	39	theme	Immulix	757:763	arg1	minor					799:803	minor	799:803	minor	799:803	The effects of Immulix and/or increased fat content were minor.
35022427	6	39	theme	Immulix	757:763	arg1	effects					746:752	The effects	742:752	The effects of Immulix and/or increased fat content	742:792	The effects of Immulix and/or increased fat content were minor.
35022427	3	40	dep	size	419:422	arg1	e.g.					410:413	e.g.	410:413	e.g.	410:413	Also, diet, e.g. the size of the fat fraction, is known to modulate the GM.
35022427	4	41	theme	increased	528:536	arg1	content					550:556	increased dietary fat content	528:556	increased dietary fat content	528:556	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	4	42	theme	fat	546:548	arg1	content					550:556	increased dietary fat content	528:556	increased dietary fat content	528:556	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	13	43	dep	cytokines	1529:1537	arg1	content					1595:1601	increased dietary fat content	1573:1601	increased dietary fat content	1573:1601	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	43	dep	cytokines	1529:1537	arg1	feeding					1562:1568	Immulix feeding	1554:1568	Immulix feeding	1554:1568	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	43	dep	cytokines	1529:1537	arg1	cytokines					1529:1537	cytokines	1529:1537	cytokines	1529:1537	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	0	44	contain	has	49:51	arg1	Immulix					40:46	Immulix	40:46	Immulix	40:46	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	0	44	contain	has	49:51	arg1	supplement					28:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	0	44	contain	has	49:51	arg2	impact					59:64	minor impact	53:64	minor impact	53:64	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	2	45	theme	dietary	264:270	arg1	Oligosaccharides					218:233	Oligosaccharides	218:233	Oligosaccharides	218:233	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	2	45	theme	dietary	264:270	arg1	supplement					272:281	dietary supplement	264:281	dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM)	264:395	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	11	46	theme	Prevotella	1336:1345	arg1	spp					1347:1349	an unclassified Prevotella spp	1320:1349	an unclassified Prevotella spp	1320:1349	Increased dietary fat content upregulated Lactobacillus animalis but downregulated an unclassified Prevotella spp.
35022427	9	47	theme	increased	1094:1102	arg1	fat					1112:1114	increased dietary fat	1094:1114	increased dietary fat	1094:1114	The diet with increased dietary fat increased Il1b but decreased Cd8a gene expression in the spleen.
35022427	4	48	from	vaccine	596:602	arg1	mice					621:624	48 BALB/cJTac mice	607:624	48 BALB/cJTac mice	607:624	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	13	49	contain	have	1611:1614	arg1	content					1595:1601	increased dietary fat content	1573:1601	increased dietary fat content	1573:1601	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	49	contain	have	1611:1614	arg1	feeding					1562:1568	Immulix feeding	1554:1568	Immulix feeding	1554:1568	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	49	contain	have	1611:1614	arg1	cytokines					1529:1537	cytokines	1529:1537	cytokines	1529:1537	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	49	contain	have	1611:1614	arg1	titers					1547:1552	IgG titers	1543:1552	IgG titers	1543:1552	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	49	contain	have	1611:1614	arg2	effects					1642:1648	any biologically relevant effects	1616:1648	any biologically relevant effects on tetanus vaccine responses in this experiment in mice	1616:1704	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	11	50	theme	dietary	1247:1253	arg1	content					1259:1265	Increased dietary fat content	1237:1265	Increased dietary fat content	1237:1265	Increased dietary fat content upregulated Lactobacillus animalis but downregulated an unclassified Prevotella spp.
35022427	8	51	from	effect	978:983	arg1	expression					988:997	expression	988:997	expression of Cd4 and Foxp3 in ileum	988:1023	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	11	52	theme	Lactobacillus	1279:1291	arg1	animalis					1293:1300	Lactobacillus animalis	1279:1300	Lactobacillus animalis	1279:1300	Increased dietary fat content upregulated Lactobacillus animalis but downregulated an unclassified Prevotella spp.
35022427	9	53	theme	gene	1150:1153	arg1	expression					1155:1164	Cd8a gene expression	1145:1164	Cd8a gene expression in the spleen	1145:1178	The diet with increased dietary fat increased Il1b but decreased Cd8a gene expression in the spleen.
35022427	13	54	theme	cell	1515:1518	arg1	markers					1520:1526	immune cell markers	1508:1526	immune cell markers	1508:1526	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	1	55	from	effect	182:187	arg1	response					208:215	vaccine response	200:215	vaccine response	200:215	Several mammalian species are vaccinated in early life, but little is known about the effect of diet on vaccine response.
35022427	1	56	theme	Several	96:102	arg1	species					114:120	Several mammalian species	96:120	Several mammalian species	96:120	Several mammalian species are vaccinated in early life, but little is known about the effect of diet on vaccine response.
35022427	8	57	from	expression	988:997	arg1	ileum					1019:1023	ileum	1019:1023	ileum	1019:1023	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	4	58	theme	GM	634:635	arg1	modulation					637:646	GM modulation	634:646	GM modulation	634:646	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	5	59	theme	IgG	686:688	arg1	titers					690:695	significantly higher IgG titers	665:695	significantly higher IgG titers with higher variation	665:717	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	13	60	theme	minor	1488:1492	arg1	influences					1494:1503	some minor influences	1483:1503	some minor influences on immune cell markers	1483:1526	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	5	61	with	titers	690:695	arg1	variation					709:717	higher variation	702:717	higher variation	702:717	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	0	62	theme	vaccine	69:75	arg1	responses					77:85	vaccine responses	69:85	vaccine responses in mice	69:93	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	7	63	theme	gene	918:921	arg1	expression					923:932	Il1b gene expression	913:932	Il1b gene expression	913:932	Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen.
35022427	1	64	theme	early	140:144	arg1	life					146:149	early life	140:149	early life	140:149	Several mammalian species are vaccinated in early life, but little is known about the effect of diet on vaccine response.
35022427	13	65	theme	vaccine	1661:1667	arg1	responses					1669:1677	tetanus vaccine responses	1653:1677	tetanus vaccine responses in this experiment	1653:1696	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	8	66	with	diet	1055:1058	arg1	fat					1075:1077	increased fat	1065:1077	increased fat	1065:1077	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	7	67	dep	weeks	863:867	arg1	vaccination					885:895	secondary vaccination	875:895	secondary vaccination	875:895	Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen.
35022427	2	68	theme	due	305:307	arg1	individuals					293:303	young individuals	287:303	young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM)	287:395	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	5	69	contain	had	661:663	arg1	mice					656:659	Female mice	649:659	Female mice	649:659	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	5	69	contain	had	661:663	arg2	titers					690:695	significantly higher IgG titers	665:695	significantly higher IgG titers with higher variation	665:717	Female mice had significantly higher IgG titers with higher variation compared to male mice.
35022427	13	70	theme	relevant	1633:1640	arg1	effects					1642:1648	any biologically relevant effects	1616:1648	any biologically relevant effects on tetanus vaccine responses in this experiment in mice	1616:1704	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	2	71	theme	microbiota	381:390	arg1	modulation					363:372	modulation	363:372	modulation of gut microbiota (GM)	363:395	Oligosaccharides are increasingly proposed as dietary supplement for young individuals due to their anti-inflammatory potential elicited through modulation of gut microbiota (GM).
35022427	0	72	theme	equine	16:21	arg1	Immulix					40:46	Immulix	40:46	Immulix	40:46	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	0	72	theme	equine	16:21	arg1	supplement					28:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement	0:37	Oligosaccharide equine feed supplement, Immulix, has minor impact on vaccine responses in mice.
35022427	8	73	theme	Cd4	1002:1004	arg1	expression					988:997	expression	988:997	expression of Cd4 and Foxp3 in ileum	988:1023	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	7	74	from	titers	838:843	arg1	mice					853:856	male mice	848:856	male mice	848:856	Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen.
35022427	8	75	theme	Foxp3	1010:1014	arg1	expression					988:997	expression	988:997	expression of Cd4 and Foxp3 in ileum	988:1023	Immulix had a downregulating effect on expression of Cd4 and Foxp3 in ileum only if the mice were fed the diet with increased fat.
35022427	10	76	theme	GM	1207:1208	arg1	composition					1210:1220	GM composition	1207:1220	GM composition	1207:1220	Immulix and diet affected GM composition significantly.
35022427	6	77	theme	fat	782:784	arg1	content					786:792	increased fat content	772:792	increased fat content	772:792	The effects of Immulix and/or increased fat content were minor.
35022427	13	78	theme	dietary	1583:1589	arg1	content					1595:1601	increased dietary fat content	1573:1601	increased dietary fat content	1573:1601	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	13	78	theme	dietary	1583:1589	arg1	cytokines					1529:1537	cytokines	1529:1537	cytokines	1529:1537	It is concluded that in spite of some minor influences on immune cell markers, cytokines and IgG titers Immulix feeding or increased dietary fat content did not have any biologically relevant effects on tetanus vaccine responses in this experiment in mice.
35022427	9	79	with	diet	1084:1087	arg1	fat					1112:1114	increased dietary fat	1094:1114	increased dietary fat	1094:1114	The diet with increased dietary fat increased Il1b but decreased Cd8a gene expression in the spleen.
35022427	4	80	theme	dietary	538:544	arg1	content					550:556	increased dietary fat content	528:556	increased dietary fat content	528:556	We tested if an oligosaccharide diet (Immulix) and/or increased dietary fat content affected antibody titers to a tetanus vaccine in 48 BALB/cJTac mice through GM modulation.
35022427	3	81	theme	fraction	435:442	arg1	size					419:422	the size	415:422	the size of the fat fraction	415:442	Also, diet, e.g. the size of the fat fraction, is known to modulate the GM.
35022427	7	82	theme	IgG	834:836	arg1	titers					838:843	IgG titers	834:843	IgG titers in male mice	834:856	Immulix negatively affected IgG titers in male mice four weeks after secondary vaccination but upregulated Il1b gene expression in the spleen.
35022427	1	83	theme	diet	192:195	arg1	effect					182:187	the effect	178:187	the effect of diet on vaccine response	178:215	Several mammalian species are vaccinated in early life, but little is known about the effect of diet on vaccine response.
35022427	11	84	theme	unclassified	1323:1334	arg1	spp					1347:1349	an unclassified Prevotella spp	1320:1349	an unclassified Prevotella spp	1320:1349	Increased dietary fat content upregulated Lactobacillus animalis but downregulated an unclassified Prevotella spp.
35022427	1	85	theme	vaccine	200:206	arg1	response					208:215	vaccine response	200:215	vaccine response	200:215	Several mammalian species are vaccinated in early life, but little is known about the effect of diet on vaccine response.
36750451	4	0	theme	protein/vegetable	525:541	arg1	blends					566:571	dairy protein/vegetable protein/polysaccharide blends	519:571	dairy protein/vegetable protein/polysaccharide blends	519:571	No reports regarding the use of dairy protein/vegetable protein/polysaccharide blends as wall material for the microencapsulation of CO were published.
36750451	2	1	theme	fatty	301:305	arg1	acids					307:311	polyunsaturated fatty acids	285:311	polyunsaturated fatty acids	285:311	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	4	2	theme	blends	566:571	arg1	use					512:514	the use	508:514	the use of dairy protein/vegetable protein/polysaccharide blends as wall material for the microencapsulation of CO	508:621	No reports regarding the use of dairy protein/vegetable protein/polysaccharide blends as wall material for the microencapsulation of CO were published.
36750451	3	3	theme	alternative	436:446	arg1	materials					453:461	alternative wall materials	436:461	alternative wall materials for microencapsulation	436:484	This justifies the study of alternative wall materials for microencapsulation.
36750451	1	4	theme	α-linolenic	232:242	arg1	acid					244:247	α-linolenic acid	232:247	α-linolenic acid (60-66 %)	232:257	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	1	4	theme	α-linolenic	232:242	arg1	%					256:256	60-66 %	250:256	60-66 %	250:256	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	12	5	theme	omega-3-rich	1765:1776	arg1	carriers					1778:1785	promising omega-3-rich carriers	1755:1785	promising omega-3-rich carriers for being incorporated into functional foods	1755:1830	CONCLUSION Microcapsules developed from complex coacervates based on the ternary blend represent promising omega-3-rich carriers for being incorporated into functional foods.
36750451	6	6	theme	CC	810:811	arg1	process					814:820	The complex coacervation (CC) process	784:820	The complex coacervation (CC) process	784:820	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	7	theme	stirring	908:915	arg1	speed					917:921	stirring speed	908:921	stirring speed (600 rpm)	908:931	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	7	theme	stirring	908:915	arg1	rpm					928:930	600 rpm	924:930	600 rpm	924:930	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	7	theme	stirring	908:915	arg1	min					946:948	30 min	943:948	30 min	943:948	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	5	8	theme	gum	751:753	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	1	9	theme	Chia	165:168	arg1	oil					170:172	BACKGROUND Chia oil	154:172	BACKGROUND Chia oil (CO)	154:177	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	1	9	theme	Chia	165:168	arg1	CO					175:176	CO	175:176	CO	175:176	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	6	10	theme	coacervation	796:807	arg1	process					814:820	The complex coacervation (CC) process	784:820	The complex coacervation (CC) process	784:820	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	0	11	theme	soy	71:73	arg1	isolate					83:89	soy protein isolate	71:89	soy protein isolate	71:89	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	12	12	theme	ternary	1731:1737	arg1	blend					1739:1743	the ternary blend	1727:1743	the ternary blend	1727:1743	CONCLUSION Microcapsules developed from complex coacervates based on the ternary blend represent promising omega-3-rich carriers for being incorporated into functional foods.
36750451	6	13	theme	solid	851:855	arg1	w/v					870:872	30% w/v	866:872	30% w/v	866:872	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	13	theme	solid	851:855	arg1	content					857:863	total solid content	845:863	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	10	14	theme	encapsulation	1365:1377	arg1	%					1402:1402	up to 79.88%	1391:1402	up to 79.88%	1391:1402	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	14	theme	encapsulation	1365:1377	arg1	efficiency					1379:1388	encapsulation efficiency	1365:1388	encapsulation efficiency (up to 79.88%)	1365:1403	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	11	15	theme	fatty	1599:1603	arg1	composition					1610:1620	the fatty acid composition	1595:1620	the fatty acid composition of bulk and microencapsulated oils	1595:1655	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	5	16	theme	blend	760:764	arg1	behavior					673:680	the behavior	669:680	the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material	669:781	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	6	17	dep	pH	835:836	arg1	w/v					870:872	30% w/v	866:872	30% w/v	866:872	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	min					946:948	30 min	943:948	30 min	943:948	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	w/w/w					900:904	8/1/1 w/w/w	894:904	8/1/1 w/w/w	894:904	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	rpm					928:930	600 rpm	924:930	600 rpm	924:930	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	content					857:863	total solid content	845:863	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	ratio					887:891	WPC/SPI/AG ratio	876:891	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	temperature					957:967	room temperature	952:967	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	speed					917:921	stirring speed	908:921	stirring speed (600 rpm)	908:931	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	17	dep	pH	835:836	arg1	time					937:940	time	937:940	time	937:940	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	4	18	theme	CO	620:621	arg1	microencapsulation					598:615	the microencapsulation	594:615	the microencapsulation of CO	594:621	No reports regarding the use of dairy protein/vegetable protein/polysaccharide blends as wall material for the microencapsulation of CO were published.
36750451	0	19	theme	oxidative	121:129	arg1	stability					131:139	the oxidative stability	117:139	the oxidative stability of chia oil	117:151	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	5	20	theme	wall	769:772	arg1	material					774:781	wall material	769:781	wall material	769:781	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	9	21	theme	encapsulation	1270:1282	arg1	efficiency					1284:1293	the encapsulation efficiency	1266:1293	the encapsulation efficiency due to its high viscosity	1266:1319	Meanwhile, SPI contributed to the increase of the encapsulation efficiency due to its high viscosity.
36750451	0	22	theme	oil	149:151	arg1	stability					131:139	the oxidative stability	117:139	the oxidative stability of chia oil	117:151	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	9	23	theme	due	1295:1297	arg1	efficiency					1284:1293	the encapsulation efficiency	1266:1293	the encapsulation efficiency due to its high viscosity	1266:1319	Meanwhile, SPI contributed to the increase of the encapsulation efficiency due to its high viscosity.
36750451	6	24	theme	WPC/SPI/AG	876:885	arg1	w/w/w					900:904	8/1/1 w/w/w	894:904	8/1/1 w/w/w	894:904	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	24	theme	WPC/SPI/AG	876:885	arg1	ratio					887:891	WPC/SPI/AG ratio	876:891	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	12	25	theme	functional	1815:1824	arg1	foods					1826:1830	functional foods	1815:1830	functional foods	1815:1830	CONCLUSION Microcapsules developed from complex coacervates based on the ternary blend represent promising omega-3-rich carriers for being incorporated into functional foods.
36750451	0	26	theme	protein	75:81	arg1	isolate					83:89	soy protein isolate	71:89	soy protein isolate	71:89	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	6	27	theme	%	868:868	arg1	w/v					870:872	30% w/v	866:872	30% w/v	866:872	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	27	theme	%	868:868	arg1	content					857:863	total solid content	845:863	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	8	28	theme	well-known	1124:1133	arg1	ability					1150:1156	the well-known matrix-forming ability	1120:1156	the well-known matrix-forming ability of AG	1120:1162	Besides the well-known matrix-forming ability of AG, WPC helped increase the OSI of microencapsulated oils.
36750451	5	29	theme	protein	692:698	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	10	30	theme	peak	1500:1503	arg1	temperatures					1505:1516	the denaturation peak temperatures	1483:1516	the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C)	1483:1548	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	2	31	theme	CO	346:347	arg1	incorporation					324:336	the incorporation	320:336	the incorporation of bulk CO in food products	320:364	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	0	32	theme	whey	45:48	arg1	concentrate					58:68	whey protein concentrate	45:68	whey protein concentrate	45:68	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	7	33	theme	CO	1017:1018	arg1	OSI					1009:1011	OSI	1009:1011	OSI	1009:1011	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	7	33	theme	CO	1017:1018	arg1	index					1002:1006	The oxidative stability index	978:1006	The oxidative stability index (OSI) of CO (3.25±0.16 h)	978:1032	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	8	34	theme	AG	1161:1162	arg1	ability					1150:1156	the well-known matrix-forming ability	1120:1156	the well-known matrix-forming ability of AG	1120:1162	Besides the well-known matrix-forming ability of AG, WPC helped increase the OSI of microencapsulated oils.
36750451	0	35	theme	Complex	0:6	arg1	coacervation					8:19	Complex coacervation	0:19	Complex coacervation	0:19	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	1	36	theme	acid	244:247	arg1	source					222:227	the richest vegetable source	200:227	the richest vegetable source of α-linolenic acid (60-66 %)	200:257	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	5	37	theme	AG	756:757	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	6	38	theme	complex	788:794	arg1	process					814:820	The complex coacervation (CC) process	784:820	The complex coacervation (CC) process	784:820	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	2	39	theme	food	352:355	arg1	products					357:364	food products	352:364	food products	352:364	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	11	40	theme	oils	1652:1655	arg1	composition					1610:1620	the fatty acid composition	1595:1620	the fatty acid composition of bulk and microencapsulated oils	1595:1655	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	2	41	theme	oxidation	397:405	arg1	probability					382:392	its high probability	373:392	its high probability of oxidation	373:405	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	2	42	theme	polyunsaturated	285:299	arg1	acids					307:311	polyunsaturated fatty acids	285:311	polyunsaturated fatty acids	285:311	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	0	43	theme	protein	50:56	arg1	concentrate					58:68	whey protein concentrate	45:68	whey protein concentrate	45:68	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	8	44	theme	microencapsulated	1196:1212	arg1	oils					1214:1217	microencapsulated oils	1196:1217	microencapsulated oils	1196:1217	Besides the well-known matrix-forming ability of AG, WPC helped increase the OSI of microencapsulated oils.
36750451	11	45	theme	acid	1605:1608	arg1	composition					1610:1620	the fatty acid composition	1595:1620	the fatty acid composition of bulk and microencapsulated oils	1595:1655	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	7	46	theme	stability	992:1000	arg1	OSI					1009:1011	OSI	1009:1011	OSI	1009:1011	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	7	46	theme	stability	992:1000	arg1	index					1002:1006	The oxidative stability index	978:1006	The oxidative stability index (OSI) of CO (3.25±0.16 h)	978:1032	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	10	47	from	h	1432:1432	arg1	stability					1447:1455	thermal stability	1439:1455	thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C)	1439:1548	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	47	from	h	1432:1432	arg1	%					1402:1402	up to 79.88%	1391:1402	up to 79.88%	1391:1402	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	47	from	h	1432:1432	arg1	efficiency					1379:1388	encapsulation efficiency	1365:1388	encapsulation efficiency (up to 79.88%)	1365:1403	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	47	from	h	1432:1432	arg1	OSIs					1406:1409	OSIs	1406:1409	OSIs (from 11.25 to 12.52 h)	1406:1433	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	48	theme	Enhanced	1322:1329	arg1	properties					1331:1340	Enhanced properties	1322:1340	Enhanced properties	1322:1340	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	49	dep	79.88	1397:1401	arg1	to					1394:1395	to	1394:1395	to	1394:1395	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	2	50	theme	acids	307:311	arg1	content					274:280	this content	269:280	this content of polyunsaturated fatty acids	269:311	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	1	51	theme	vegetable	212:220	arg1	source					222:227	the richest vegetable source	200:227	the richest vegetable source of α-linolenic acid (60-66 %)	200:257	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	4	52	theme	dairy	519:523	arg1	blends					566:571	dairy protein/vegetable protein/polysaccharide blends	519:571	dairy protein/vegetable protein/polysaccharide blends	519:571	No reports regarding the use of dairy protein/vegetable protein/polysaccharide blends as wall material for the microencapsulation of CO were published.
36750451	4	53	theme	protein/polysaccharide	543:564	arg1	blends					566:571	dairy protein/vegetable protein/polysaccharide blends	519:571	dairy protein/vegetable protein/polysaccharide blends	519:571	No reports regarding the use of dairy protein/vegetable protein/polysaccharide blends as wall material for the microencapsulation of CO were published.
36750451	10	54	theme	denaturation	1487:1498	arg1	temperatures					1505:1516	the denaturation peak temperatures	1483:1516	the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C)	1483:1548	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	1	55	theme	BACKGROUND	154:163	arg1	oil					170:172	BACKGROUND Chia oil	154:172	BACKGROUND Chia oil (CO)	154:177	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	1	55	theme	BACKGROUND	154:163	arg1	CO					175:176	CO	175:176	CO	175:176	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36750451	12	56	theme	promising	1755:1763	arg1	carriers					1778:1785	promising omega-3-rich carriers	1755:1785	promising omega-3-rich carriers for being incorporated into functional foods	1755:1830	CONCLUSION Microcapsules developed from complex coacervates based on the ternary blend represent promising omega-3-rich carriers for being incorporated into functional foods.
36750451	5	57	theme	protein	722:728	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	6	58	theme	room	952:955	arg1	temperature					957:967	room temperature	952:967	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	10	59	theme	WPC	1521:1523	arg1	temperatures					1505:1516	the denaturation peak temperatures	1483:1516	the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C)	1483:1548	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	6	60	theme	total	845:849	arg1	w/v					870:872	30% w/v	866:872	30% w/v	866:872	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	6	60	theme	total	845:849	arg1	content					857:863	total solid content	845:863	pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature	835:967	The complex coacervation (CC) process was studied: pH (4.0); total solid content (30% w/v); WPC/SPI/AG ratio (8/1/1 w/w/w); stirring speed (600 rpm) and time (30 min); room temperature.
36750451	10	61	dep	86.00	1540:1544	arg1	to					1537:1538	to	1537:1538	to	1537:1538	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	5	62	theme	whey	687:690	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	0	63	theme	chia	144:147	arg1	oil					149:151	chia oil	144:151	chia oil	144:151	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	12	64	theme	complex	1698:1704	arg1	coacervates					1706:1716	complex coacervates	1698:1716	complex coacervates based on the ternary blend	1698:1743	CONCLUSION Microcapsules developed from complex coacervates based on the ternary blend represent promising omega-3-rich carriers for being incorporated into functional foods.
36750451	9	65	theme	efficiency	1284:1293	arg1	increase					1254:1261	the increase	1250:1261	the increase of the encapsulation efficiency due to its high viscosity	1250:1319	Meanwhile, SPI contributed to the increase of the encapsulation efficiency due to its high viscosity.
36750451	5	66	theme	WPC	713:715	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	5	67	theme	/soy	717:720	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	9	68	theme	high	1306:1309	arg1	viscosity					1311:1319	its high viscosity	1302:1319	its high viscosity	1302:1319	Meanwhile, SPI contributed to the increase of the encapsulation efficiency due to its high viscosity.
36750451	7	69	dep	RESULTS	970:976	arg1	increased					1052:1060	increased	1052:1060	was significantly increased after microencapsulation (around 4 times higher)	1034:1109	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	12	70	theme	CONCLUSION	1658:1667	arg1	Microcapsules					1669:1681	CONCLUSION Microcapsules	1658:1681	CONCLUSION Microcapsules developed from complex coacervates based on the ternary blend	1658:1743	CONCLUSION Microcapsules developed from complex coacervates based on the ternary blend represent promising omega-3-rich carriers for being incorporated into functional foods.
36750451	0	71	theme	arabic	95:100	arg1	gum					102:104	arabic gum	95:104	arabic gum	95:104	Complex coacervation and freeze-drying using whey protein concentrate, soy protein isolate and arabic gum to improve the oxidative stability of chia oil.
36750451	5	72	theme	concentrate	700:710	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	11	73	located	observed	1583:1590	arg2	differences					1566:1576	No significant differences	1551:1576	No significant differences	1551:1576	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	11	73	located	observed	1583:1590	arg1	composition					1610:1620	the fatty acid composition	1595:1620	the fatty acid composition of bulk and microencapsulated oils	1595:1655	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	8	74	theme	matrix-forming	1135:1148	arg1	ability					1150:1156	the well-known matrix-forming ability	1120:1156	the well-known matrix-forming ability of AG	1120:1162	Besides the well-known matrix-forming ability of AG, WPC helped increase the OSI of microencapsulated oils.
36750451	2	75	theme	bulk	341:344	arg1	CO					346:347	bulk CO	341:347	bulk CO	341:347	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	5	76	theme	/arabic	743:749	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	7	77	theme	higher	1103:1108	arg1	times					1097:1101	4 times	1095:1101	4 times higher	1095:1108	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	2	78	theme	high	377:380	arg1	probability					382:392	its high probability	373:392	its high probability of oxidation	373:405	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	11	79	theme	bulk	1625:1628	arg1	composition					1610:1620	the fatty acid composition	1595:1620	the fatty acid composition of bulk and microencapsulated oils	1595:1655	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	3	80	theme	materials	453:461	arg1	study					427:431	the study	423:431	the study of alternative wall materials for microencapsulation	423:484	This justifies the study of alternative wall materials for microencapsulation.
36750451	5	81	theme	isolate	730:736	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	5	82	theme	SPI	739:741	arg1	blend					760:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend	685:764	Therefore, this work analyzed the behavior of a whey protein concentrate (WPC)/soy protein isolate (SPI)/arabic gum (AG) blend as wall material.
36750451	11	83	theme	significant	1554:1564	arg1	differences					1566:1576	No significant differences	1551:1576	No significant differences	1551:1576	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	2	84	from	incorporation	324:336	arg1	products					357:364	food products	352:364	food products	352:364	However, this content of polyunsaturated fatty acids limits the incorporation of bulk CO in food products due to its high probability of oxidation.
36750451	10	85	dep	12.52	1426:1430	arg1	to					1423:1424	to	1423:1424	to	1423:1424	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	3	86	theme	wall	448:451	arg1	materials					453:461	alternative wall materials	436:461	alternative wall materials for microencapsulation	436:484	This justifies the study of alternative wall materials for microencapsulation.
36750451	10	87	theme	thermal	1439:1445	arg1	stability					1447:1455	thermal stability	1439:1455	thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C)	1439:1548	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	4	88	theme	wall	576:579	arg1	material					581:588	wall material	576:588	wall material for the microencapsulation of CO	576:621	No reports regarding the use of dairy protein/vegetable protein/polysaccharide blends as wall material for the microencapsulation of CO were published.
36750451	10	89	theme	microcapsules	1460:1472	arg1	stability					1447:1455	thermal stability	1439:1455	thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C)	1439:1548	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	89	theme	microcapsules	1460:1472	arg1	%					1402:1402	up to 79.88%	1391:1402	up to 79.88%	1391:1402	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	89	theme	microcapsules	1460:1472	arg1	efficiency					1379:1388	encapsulation efficiency	1365:1388	encapsulation efficiency (up to 79.88%)	1365:1403	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	10	89	theme	microcapsules	1460:1472	arg1	OSIs					1406:1409	OSIs	1406:1409	OSIs (from 11.25 to 12.52 h)	1406:1433	Enhanced properties were observed with CC: encapsulation efficiency (up to 79.88%), OSIs (from 11.25 to 12.52 h) and thermal stability of microcapsules given by the denaturation peak temperatures of WPC (from 77.12 to 86.00 °C).
36750451	11	90	theme	microencapsulated	1634:1650	arg1	oils					1652:1655	microencapsulated oils	1634:1655	microencapsulated oils	1634:1655	No significant differences were observed in the fatty acid composition of bulk and microencapsulated oils.
36750451	8	91	theme	oils	1214:1217	arg1	OSI					1189:1191	the OSI	1185:1191	the OSI of microencapsulated oils	1185:1217	Besides the well-known matrix-forming ability of AG, WPC helped increase the OSI of microencapsulated oils.
36750451	7	92	theme	oxidative	982:990	arg1	OSI					1009:1011	OSI	1009:1011	OSI	1009:1011	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	7	92	theme	oxidative	982:990	arg1	index					1002:1006	The oxidative stability index	978:1006	The oxidative stability index (OSI) of CO (3.25±0.16 h)	978:1032	RESULTS The oxidative stability index (OSI) of CO (3.25±0.16 h) was significantly increased after microencapsulation (around 4 times higher).
36750451	1	93	theme	richest	204:210	arg1	source					222:227	the richest vegetable source	200:227	the richest vegetable source of α-linolenic acid (60-66 %)	200:257	BACKGROUND Chia oil (CO) is popular for being the richest vegetable source of α-linolenic acid (60-66 %).
36420169	8	0	theme	construct	1199:1207	arg1	properties					1176:1185	The biophysical properties	1160:1185	The biophysical properties of this new construct	1160:1207	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	6	1	theme	structures	1002:1011	arg1	formation					971:979	the formation	967:979	the formation of proto-tissue-like structures	967:1011	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	5	2	theme	β-propeller	653:663	arg1	domain					665:670	the β-propeller domain	649:670	the β-propeller domain from Ralstonia solanacearum	649:698	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	5	3	theme	β-trefoil	708:716	arg1	domain					718:723	the β-trefoil domain	704:723	the β-trefoil domain from fungus Marasmius oreades	704:753	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	6	4	theme	giant	926:930	arg1	vesicles					944:951	glycosylated giant unilamellar vesicles	913:951	glycosylated giant unilamellar vesicles	913:951	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	8	5	theme	new	1195:1197	arg1	construct					1199:1207	this new construct	1190:1207	this new construct	1190:1207	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	9	6	theme	proteins	1324:1331	arg1	profiles					1308:1315	Denaturation profiles	1295:1315	Denaturation profiles of the proteins	1295:1331	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	6	7	theme	protein	880:886	arg1	activity					888:895	The protein activity	876:895	The protein activity	876:895	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	6	8	theme	protocells	1043:1052	arg1	cross-linking					1021:1033	cross-linking	1021:1033	cross-linking of such protocells	1021:1052	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	5	9	theme	novel	586:590	arg1	lectin					603:608	The novel engineered lectin	582:608	The novel engineered lectin	582:608	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	5	9	theme	novel	586:590	arg1	specific					759:766	specific	759:766	specific	759:766	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	6	10	theme	such	1038:1041	arg1	protocells					1043:1052	such protocells	1038:1052	such protocells	1038:1052	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	4	11	theme	lectins	501:507	arg1	concept					470:476	the concept	466:476	the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity	466:579	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	5	12	theme	Ralstonia	677:685	arg1	solanacearum					687:698	Ralstonia solanacearum	677:698	Ralstonia solanacearum	677:698	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	3	13	theme	binding	358:364	arg1	proteins					366:373	glycan binding proteins	351:373	glycan binding proteins	351:373	In synthetic glycobiology, the engineering of glycan binding proteins is being exploited for producing tools with precise topology and specificity.
36420169	4	14	theme	chimeric	492:499	arg1	lectin					522:527	Janus lectin	516:527	Janus lectin	516:527	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	4	14	theme	chimeric	492:499	arg1	lectins					501:507	engineered chimeric lectins	481:507	engineered chimeric lectins	481:507	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	5	15	theme	fusion	626:631	arg1	protein					633:639	a fusion protein	624:639	a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades	624:753	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	3	16	theme	proteins	366:373	arg1	engineering					336:346	the engineering	332:346	the engineering of glycan binding proteins	332:373	In synthetic glycobiology, the engineering of glycan binding proteins is being exploited for producing tools with precise topology and specificity.
36420169	1	17	theme	Synthetic	86:94	arg1	field					125:129	a rapidly growing field	107:129	a rapidly growing field with applications in biotechnology and biomedicine	107:180	Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine.
36420169	1	17	theme	Synthetic	86:94	arg1	biology					96:102	Synthetic biology	86:102	Synthetic biology	86:102	Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine.
36420169	9	18	theme	Denaturation	1295:1306	arg1	profiles					1308:1315	Denaturation profiles	1295:1315	Denaturation profiles of the proteins	1295:1331	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	0	19	theme	Janus	10:14	arg1	lectins					16:22	Janus lectins architecture	10:35	Janus lectins architecture: Characterization and application to protocells	10:83	Extending Janus lectins architecture: Characterization and application to protocells.
36420169	0	19	theme	Janus	10:14	arg1	Characterization					38:53	Characterization	38:53	Characterization	38:53	Extending Janus lectins architecture: Characterization and application to protocells.
36420169	0	19	theme	Janus	10:14	arg1	application					59:69	application	59:69	application to protocells	59:83	Extending Janus lectins architecture: Characterization and application to protocells.
36420169	9	20	theme	significant	1370:1380	arg1	role					1382:1385	a significant role	1368:1385	a significant role	1368:1385	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	1	21	from	applications	136:147	arg1	biotechnology					152:164	biotechnology	152:164	biotechnology	152:164	Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine.
36420169	1	21	from	applications	136:147	arg1	biomedicine					170:180	biomedicine	170:180	biomedicine	170:180	Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine.
36420169	9	22	theme	each	1359:1362	arg1	fold					1351:1354	the fold	1347:1354	the fold of each	1347:1362	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	6	23	theme	glycosylated	913:924	arg1	vesicles					944:951	glycosylated giant unilamellar vesicles	913:951	glycosylated giant unilamellar vesicles	913:951	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	4	24	with	concept	470:476	arg1	valency					545:551	increased valency	535:551	increased valency	535:551	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	4	24	with	concept	470:476	arg1	specificity					569:579	additional specificity	558:579	additional specificity	558:579	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	4	25	theme	additional	558:567	arg1	specificity					569:579	additional specificity	558:579	additional specificity	558:579	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	1	26	with	field	125:129	arg1	applications					136:147	applications	136:147	applications in biotechnology and biomedicine	136:180	Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine.
36420169	5	27	theme	engineered	592:601	arg1	lectin					603:608	The novel engineered lectin	582:608	The novel engineered lectin	582:608	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	5	27	theme	engineered	592:601	arg1	specific					759:766	specific	759:766	specific	759:766	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	8	28	theme	Janus	1253:1257	arg1	RSL-CBM77Rf					1282:1292	RSL-CBM77Rf	1282:1292	RSL-CBM77Rf	1282:1292	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	8	28	theme	Janus	1253:1257	arg1	RSL-CBM40					1268:1276	RSL-CBM40	1268:1276	RSL-CBM40	1268:1276	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	8	28	theme	Janus	1253:1257	arg1	lectins					1259:1265	the two already existing Janus lectins	1228:1265	the two already existing Janus lectins	1228:1265	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	7	29	theme	cancer	1127:1132	arg1	cells					1134:1138	H1299 human lung epithelial cancer cells	1099:1138	H1299 human lung epithelial cancer cells	1099:1138	The engineered protein recognizes and binds H1299 human lung epithelial cancer cells by its two domains.
36420169	6	30	theme	unilamellar	932:942	arg1	vesicles					944:951	glycosylated giant unilamellar vesicles	913:951	glycosylated giant unilamellar vesicles	913:951	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	8	31	theme	existing	1244:1251	arg1	RSL-CBM77Rf					1282:1292	RSL-CBM77Rf	1282:1292	RSL-CBM77Rf	1282:1292	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	8	31	theme	existing	1244:1251	arg1	RSL-CBM40					1268:1276	RSL-CBM40	1268:1276	RSL-CBM40	1268:1276	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	8	31	theme	existing	1244:1251	arg1	lectins					1259:1265	the two already existing Janus lectins	1228:1265	the two already existing Janus lectins	1228:1265	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
36420169	4	32	dep	lectins	501:507	arg1	i.e.					510:513	engineered chimeric lectins, i.e., Janus lectin,	481:528	i.e.	510:513	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	3	33	with	tools	408:412	arg1	specificity					440:450	specificity	440:450	specificity	440:450	In synthetic glycobiology, the engineering of glycan binding proteins is being exploited for producing tools with precise topology and specificity.
36420169	3	33	with	tools	408:412	arg1	topology					427:434	precise topology	419:434	precise topology	419:434	In synthetic glycobiology, the engineering of glycan binding proteins is being exploited for producing tools with precise topology and specificity.
36420169	9	34	contain	has	1364:1366	arg1	fold					1351:1354	the fold	1347:1354	the fold of each	1347:1362	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	9	34	contain	has	1364:1366	arg2	role					1382:1385	a significant role	1368:1385	a significant role	1368:1385	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	6	35	theme	proto-tissue-like	984:1000	arg1	structures					1002:1011	proto-tissue-like structures	984:1011	proto-tissue-like structures	984:1011	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	7	36	theme	lung	1111:1114	arg1	cells					1134:1138	H1299 human lung epithelial cancer cells	1099:1138	H1299 human lung epithelial cancer cells	1099:1138	The engineered protein recognizes and binds H1299 human lung epithelial cancer cells by its two domains.
36420169	2	37	theme	various	191:197	arg1	approaches					199:208	various approaches	191:208	various approaches	191:208	Through various approaches, remarkable achievements, such as cell and tissue engineering, have been already accomplished.
36420169	9	38	theme	protein	1390:1396	arg1	stability					1398:1406	protein stability	1390:1406	protein stability	1390:1406	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	4	39	theme	increased	535:543	arg1	valency					545:551	increased valency	535:551	increased valency	535:551	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	7	40	theme	epithelial	1116:1125	arg1	cells					1134:1138	H1299 human lung epithelial cancer cells	1099:1138	H1299 human lung epithelial cancer cells	1099:1138	The engineered protein recognizes and binds H1299 human lung epithelial cancer cells by its two domains.
36420169	4	41	theme	engineered	481:490	arg1	lectin					522:527	Janus lectin	516:527	Janus lectin	516:527	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	4	41	theme	engineered	481:490	arg1	lectins					501:507	engineered chimeric lectins	481:507	engineered chimeric lectins	481:507	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	5	42	theme	unique	803:808	arg1	architecture					818:829	its unique protein architecture	799:829	its unique protein architecture	799:829	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	5	43	theme	protein	810:816	arg1	architecture					818:829	its unique protein architecture	799:829	its unique protein architecture	799:829	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	1	44	theme	growing	117:123	arg1	field					125:129	a rapidly growing field	107:129	a rapidly growing field with applications in biotechnology and biomedicine	107:180	Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine.
36420169	1	44	theme	growing	117:123	arg1	biology					96:102	Synthetic biology	86:102	Synthetic biology	86:102	Synthetic biology is a rapidly growing field with applications in biotechnology and biomedicine.
36420169	3	45	theme	precise	419:425	arg1	topology					427:434	precise topology	419:434	precise topology	419:434	In synthetic glycobiology, the engineering of glycan binding proteins is being exploited for producing tools with precise topology and specificity.
36420169	7	46	theme	human	1105:1109	arg1	cells					1134:1138	H1299 human lung epithelial cancer cells	1099:1138	H1299 human lung epithelial cancer cells	1099:1138	The engineered protein recognizes and binds H1299 human lung epithelial cancer cells by its two domains.
36420169	5	47	from	oreades	747:753	arg1	domain					665:670	the β-propeller domain	649:670	the β-propeller domain from Ralstonia solanacearum	649:698	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	5	47	from	oreades	747:753	arg1	domain					718:723	the β-trefoil domain	704:723	the β-trefoil domain from fungus Marasmius oreades	704:753	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	6	48	gly	glycosylated	913:924	arg1	vesicles					944:951	glycosylated giant unilamellar vesicles	913:951	glycosylated giant unilamellar vesicles	913:951	The protein activity was tested with glycosylated giant unilamellar vesicles, resulting in the formation of proto-tissue-like structures through cross-linking of such protocells.
36420169	7	49	theme	engineered	1059:1068	arg1	protein					1070:1076	The engineered protein	1055:1076	The engineered protein	1055:1076	The engineered protein recognizes and binds H1299 human lung epithelial cancer cells by its two domains.
36420169	3	50	theme	synthetic	308:316	arg1	glycobiology					318:329	synthetic glycobiology	308:329	synthetic glycobiology	308:329	In synthetic glycobiology, the engineering of glycan binding proteins is being exploited for producing tools with precise topology and specificity.
36420169	2	51	theme	remarkable	211:220	arg1	cell					244:247	cell	244:247	cell	244:247	Through various approaches, remarkable achievements, such as cell and tissue engineering, have been already accomplished.
36420169	2	51	theme	remarkable	211:220	arg1	engineering					260:270	tissue engineering	253:270	tissue engineering	253:270	Through various approaches, remarkable achievements, such as cell and tissue engineering, have been already accomplished.
36420169	2	51	theme	remarkable	211:220	arg1	achievements					222:233	remarkable achievements	211:233	remarkable achievements	211:233	Through various approaches, remarkable achievements, such as cell and tissue engineering, have been already accomplished.
36420169	0	52	dep	lectins	16:22	arg1	lectins					16:22	Janus lectins architecture	10:35	Janus lectins architecture: Characterization and application to protocells	10:83	Extending Janus lectins architecture: Characterization and application to protocells.
36420169	0	52	dep	lectins	16:22	arg1	Characterization					38:53	Characterization	38:53	Characterization	38:53	Extending Janus lectins architecture: Characterization and application to protocells.
36420169	0	52	dep	lectins	16:22	arg1	architecture					24:35	architecture	24:35	Janus lectins architecture: Characterization and application to protocells	10:83	Extending Janus lectins architecture: Characterization and application to protocells.
36420169	0	52	dep	lectins	16:22	arg1	application					59:69	application	59:69	application to protocells	59:83	Extending Janus lectins architecture: Characterization and application to protocells.
36420169	9	53	theme	protein	1440:1446	arg1	engineering					1448:1458	protein engineering	1440:1458	protein engineering	1440:1458	Denaturation profiles of the proteins indicate that the fold of each has a significant role in protein stability and should be considered during protein engineering.
36420169	7	54	theme	H1299	1099:1103	arg1	cells					1134:1138	H1299 human lung epithelial cancer cells	1099:1138	H1299 human lung epithelial cancer cells	1099:1138	The engineered protein recognizes and binds H1299 human lung epithelial cancer cells by its two domains.
36420169	5	55	from	solanacearum	687:698	arg1	domain					665:670	the β-propeller domain	649:670	the β-propeller domain from Ralstonia solanacearum	649:698	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	5	55	from	solanacearum	687:698	arg1	domain					718:723	the β-trefoil domain	704:723	the β-trefoil domain from fungus Marasmius oreades	704:753	The novel engineered lectin, assembled as a fusion protein between the β-propeller domain from Ralstonia solanacearum and the β-trefoil domain from fungus Marasmius oreades, is specific for fucose and α-galactose and its unique protein architecture allows to bind these ligands simultaneously.
36420169	3	56	theme	glycan	351:356	arg1	proteins					366:373	glycan binding proteins	351:373	glycan binding proteins	351:373	In synthetic glycobiology, the engineering of glycan binding proteins is being exploited for producing tools with precise topology and specificity.
36420169	4	57	theme	Janus	516:520	arg1	lectin					522:527	Janus lectin	516:527	Janus lectin	516:527	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	4	57	theme	Janus	516:520	arg1	lectins					501:507	engineered chimeric lectins	481:507	engineered chimeric lectins	481:507	We developed the concept of engineered chimeric lectins, i.e., Janus lectin, with increased valency, and additional specificity.
36420169	2	58	theme	tissue	253:258	arg1	engineering					260:270	tissue engineering	253:270	tissue engineering	253:270	Through various approaches, remarkable achievements, such as cell and tissue engineering, have been already accomplished.
36420169	8	59	theme	biophysical	1164:1174	arg1	properties					1176:1185	The biophysical properties	1160:1185	The biophysical properties of this new construct	1160:1207	The biophysical properties of this new construct were compared with the two already existing Janus lectins, RSL-CBM40 and RSL-CBM77Rf.
35092821	5	0	theme	strong	718:723	arg1	bonds					734:738	new strong hydrogen bonds	714:738	new strong hydrogen bonds between glycan and the protein	714:769	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	7	1	theme	commercial	1079:1088	arg1	production					1100:1109	commercial biodiesel production	1079:1109	commercial biodiesel production	1079:1109	The engineered ProRML mutant has high potential for commercial biodiesel production.
35092821	1	2	theme	active	164:169	arg1	lipase					194:199	Exploiting highly active and methanol-resistant lipase	146:199	Exploiting highly active and methanol-resistant lipase	146:199	Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production.
35092821	4	3	theme	catalytic	557:565	arg1	activity					567:574	The catalytic activity	553:574	The catalytic activity of mutants N267 and N167	553:599	The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT.
35092821	4	3	theme	catalytic	557:565	arg1	higher					622:627	higher	622:627	higher	622:627	The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT.
35092821	0	4	theme	lipase	78:83	arg1	Improvement					0:10	Improvement	0:10	Improvement of methanol tolerance	0:32	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	0	4	theme	lipase	78:83	arg1	activity					48:55	catalytic activity	38:55	catalytic activity	38:55	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	6	5	theme	rapeseed	978:985	arg1	oil					987:989	rapeseed oil	978:989	rapeseed oil	978:989	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	6	6	theme	soybean	914:920	arg1	oil					922:924	waste soybean oil	908:924	waste soybean oil	908:924	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	6	6	theme	soybean	914:920	arg1	%					905:905	81.70%	900:905	81.70% (waste soybean oil)	900:925	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	0	7	theme	one-step	89:96	arg1	synthesis					98:106	one-step synthesis	89:106	one-step synthesis of biodiesel by semi-rational design	89:143	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	6	8	theme	biodiesel	857:865	arg1	yields					867:872	biodiesel yields	857:872	biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil)	857:925	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	2	9	theme	methanol	394:401	arg1	tolerance					403:411	methanol tolerance	394:411	methanol tolerance	394:411	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	6	10	theme	waste	908:912	arg1	oil					922:924	waste soybean oil	908:924	waste soybean oil	908:924	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	6	10	theme	waste	908:912	arg1	%					905:905	81.70%	900:905	81.70% (waste soybean oil)	900:925	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	0	11	theme	biodiesel	111:119	arg1	synthesis					98:106	one-step synthesis	89:106	one-step synthesis of biodiesel by semi-rational design	89:143	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	5	12	theme	methanol	823:830	arg1	tolerance					832:840	its methanol tolerance	819:840	its methanol tolerance	819:840	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	1	13	theme	methanol-resistant	175:192	arg1	lipase					194:199	Exploiting highly active and methanol-resistant lipase	146:199	Exploiting highly active and methanol-resistant lipase	146:199	Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production.
35092821	7	14	theme	high	1060:1063	arg1	potential					1065:1073	high potential	1060:1073	high potential for commercial biodiesel production	1060:1109	The engineered ProRML mutant has high potential for commercial biodiesel production.
35092821	5	15	theme	bonds	734:738	arg1	formation					701:709	the formation	697:709	the formation of new strong hydrogen bonds between glycan and the protein	697:769	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	4	16	theme	mutants	579:585	arg1	activity					567:574	The catalytic activity	553:574	The catalytic activity of mutants N267 and N167	553:599	The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT.
35092821	4	16	theme	mutants	579:585	arg1	higher					622:627	higher	622:627	higher	622:627	The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT.
35092821	3	17	theme	%	461:461	arg1	activity					463:470	64% activity	459:470	64% activity	459:470	Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT.
35092821	7	18	theme	ProRML	1042:1047	arg1	mutant					1049:1054	The engineered ProRML mutant	1027:1054	The engineered ProRML mutant	1027:1054	The engineered ProRML mutant has high potential for commercial biodiesel production.
35092821	5	19	theme	hydrogen	725:732	arg1	bonds					734:738	new strong hydrogen bonds	714:738	new strong hydrogen bonds between glycan and the protein	714:769	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	7	20	theme	biodiesel	1090:1098	arg1	production					1100:1109	commercial biodiesel production	1079:1109	commercial biodiesel production	1079:1109	The engineered ProRML mutant has high potential for commercial biodiesel production.
35092821	2	21	theme	wild	422:425	arg1	type					427:430	the wild type	418:430	the wild type (WT)	418:435	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	2	21	theme	wild	422:425	arg1	WT					433:434	WT	433:434	WT	433:434	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	0	22	theme	tolerance	24:32	arg1	Improvement					0:10	Improvement	0:10	Improvement of methanol tolerance	0:32	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	0	22	theme	tolerance	24:32	arg1	activity					48:55	catalytic activity	38:55	catalytic activity	38:55	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	6	23	theme	%	905:905	arg1	yields					867:872	biodiesel yields	857:872	biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil)	857:925	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	0	24	theme	semi-rational	124:136	arg1	design					138:143	semi-rational design	124:143	semi-rational design	124:143	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	1	25	theme	great	207:211	arg1	significance					213:224	great significance	207:224	great significance for biodiesel production	207:249	Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production.
35092821	0	26	theme	methanol	15:22	arg1	tolerance					24:32	methanol tolerance	15:32	methanol tolerance	15:32	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	6	27	theme	soybean	1013:1019	arg1	oil					1021:1023	wasted soybean oil	1006:1023	wasted soybean oil	1006:1023	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	0	28	theme	catalytic	38:46	arg1	activity					48:55	catalytic activity	38:55	catalytic activity	38:55	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	2	29	theme	semi-rational	254:266	arg1	method					287:292	A semi-rational directed evolution method	252:292	A semi-rational directed evolution method combined with N-glycosylation	252:322	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	5	30	theme	lipase	799:804	arg1	structure					786:794	the structure	782:794	the structure of lipase	782:804	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	3	31	theme	64	459:460	arg1	%					461:461	%	461:461	%	461:461	Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT.
35092821	3	32	theme	Mutant	438:443	arg1	N267					445:448	Mutant N267	438:448	Mutant N267	438:448	Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT.
35092821	1	33	theme	biodiesel	230:238	arg1	production					240:249	biodiesel production	230:249	biodiesel production	230:249	Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production.
35092821	5	34	theme	new	714:716	arg1	bonds					734:738	new strong hydrogen bonds	714:738	new strong hydrogen bonds between glycan and the protein	714:769	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	7	35	contain	has	1056:1058	arg2	potential					1065:1073	high potential	1060:1073	high potential for commercial biodiesel production	1060:1109	The engineered ProRML mutant has high potential for commercial biodiesel production.
35092821	7	35	contain	has	1056:1058	arg1	mutant					1049:1054	The engineered ProRML mutant	1027:1054	The engineered ProRML mutant	1027:1054	The engineered ProRML mutant has high potential for commercial biodiesel production.
35092821	6	36	theme	wasted	1006:1011	arg1	oil					1021:1023	wasted soybean oil	1006:1023	wasted soybean oil	1006:1023	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	5	37	theme	N267	680:683	arg1	simulations					665:675	Molecular dynamics simulations	646:675	Molecular dynamics simulations of N267	646:683	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	6	38	theme	colza	885:889	arg1	oil					891:893	colza oil	885:893	colza oil	885:893	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	6	38	theme	colza	885:889	arg1	%					882:882	99.33%	877:882	99.33% (colza oil)	877:894	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	5	39	theme	dynamics	656:663	arg1	simulations					665:675	Molecular dynamics simulations	646:675	Molecular dynamics simulations of N267	646:683	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	3	40	from	incubation	478:487	arg1	methanol					496:503	50% methanol	492:503	50% methanol	492:503	Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT.
35092821	2	41	theme	catalytic	371:379	arg1	activity					381:388	higher catalytic activity	364:388	higher catalytic activity	364:388	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	2	42	theme	higher	364:369	arg1	activity					381:388	higher catalytic activity	364:388	higher catalytic activity	364:388	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	5	43	theme	Molecular	646:654	arg1	simulations					665:675	Molecular dynamics simulations	646:675	Molecular dynamics simulations of N267	646:683	Molecular dynamics simulations of N267 showed that the formation of new strong hydrogen bonds between glycan and the protein stabilized the structure of lipase and improved its methanol tolerance.
35092821	3	44	theme	%	494:494	arg1	methanol					496:503	50% methanol	492:503	50% methanol	492:503	Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT.
35092821	3	45	theme	50	492:493	arg1	%					494:494	%	494:494	%	494:494	Mutant N267 retained 64% activity after incubation in 50% methanol for 8 h, which was 48% greater than that of WT.
35092821	0	46	theme	miehei	71:76	arg1	lipase					78:83	Rhizomucor miehei lipase	60:83	Rhizomucor miehei lipase	60:83	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	4	47	dep	mutants	579:585	arg1	N267					587:590	N267	587:590	N267	587:590	The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT.
35092821	4	47	dep	mutants	579:585	arg1	mutants					579:585	mutants N267 and N167	579:599	mutants N267 and N167	579:599	The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT.
35092821	4	47	dep	mutants	579:585	arg1	N167					596:599	N167	596:599	N167	596:599	The catalytic activity of mutants N267 and N167 was 30- and 71- fold higher than that of WT.
35092821	2	48	theme	evolution	277:285	arg1	method					287:292	A semi-rational directed evolution method	252:292	A semi-rational directed evolution method combined with N-glycosylation	252:322	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	6	49	dep	higher	952:957	arg1	%					1000:1000	44.73%	995:1000	44.73% for wasted soybean oil	995:1023	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	6	49	dep	higher	952:957	arg1	%					972:972	51.6%	968:972	51.6% for rapeseed oil	968:989	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	1	50	theme	Exploiting	146:155	arg1	lipase					194:199	Exploiting highly active and methanol-resistant lipase	146:199	Exploiting highly active and methanol-resistant lipase	146:199	Exploiting highly active and methanol-resistant lipase is of great significance for biodiesel production.
35092821	0	51	theme	Rhizomucor	60:69	arg1	lipase					78:83	Rhizomucor miehei lipase	60:83	Rhizomucor miehei lipase	60:83	Improvement of methanol tolerance and catalytic activity of Rhizomucor miehei lipase for one-step synthesis of biodiesel by semi-rational design.
35092821	6	52	theme	%	882:882	arg1	yields					867:872	biodiesel yields	857:872	biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil)	857:925	N267 achieved biodiesel yields of 99.33% (colza oil) and 81.70% (waste soybean oil) for 24 h, which was much higher than WT (51.6% for rapeseed oil and 44.73% for wasted soybean oil).
35092821	2	53	theme	directed	268:275	arg1	method					287:292	A semi-rational directed evolution method	252:292	A semi-rational directed evolution method combined with N-glycosylation	252:322	A semi-rational directed evolution method combined with N-glycosylation is reported, and all mutants exhibiting higher catalytic activity and methanol tolerance than the wild type (WT).
35092821	7	54	theme	engineered	1031:1040	arg1	mutant					1049:1054	The engineered ProRML mutant	1027:1054	The engineered ProRML mutant	1027:1054	The engineered ProRML mutant has high potential for commercial biodiesel production.
35689477	9	0	theme	altered	1713:1719	arg1	microbiota					1727:1736	the altered cecal microbiota	1709:1736	the altered cecal microbiota	1709:1736	Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats.
35689477	10	1	theme	CONCLUSION	1802:1811	arg1	FOs					1813:1815	CONCLUSION FOs	1802:1815	CONCLUSION FOs	1802:1815	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	10	1	theme	CONCLUSION	1802:1811	arg1	antioxidant					1837:1847	an antioxidant	1834:1847	an antioxidant for gut heath improvement	1834:1873	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	6	2	theme	modifier	1206:1213	arg1	subunit					1215:1221	glutamate-cysteine ligase modifier subunit	1180:1221	glutamate-cysteine ligase modifier subunit	1180:1221	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	2	theme	modifier	1206:1213	arg1	genes					1128:1132	antioxidant-related genes	1108:1132	antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1)	1108:1243	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	9	3	theme	jejunal	1762:1768	arg1	capacity					1784:1791	jejunal antioxidative capacity	1762:1791	jejunal antioxidative capacity of rats	1762:1799	Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats.
35689477	6	4	theme	glutamate-cysteine	1180:1197	arg1	ligase					1199:1204	glutamate-cysteine ligase	1180:1204	glutamate-cysteine ligase modifier subunit	1180:1221	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	10	5	theme	heath	1857:1861	arg1	improvement					1863:1873	gut heath improvement	1853:1873	gut heath improvement	1853:1873	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	5	6	theme	antioxidative	875:887	arg1	capacity					889:896	the antioxidative capacity	871:896	the antioxidative capacity of the jejunum	871:911	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	4	7	theme	standard	669:676	arg1	diet					678:681	a standard diet	667:681	a standard diet	667:681	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	0	8	theme	p38/JNK-Nrf2	120:131	arg1	pathway					143:149	the p38/JNK-Nrf2 signaling pathway	116:149	the p38/JNK-Nrf2 signaling pathway	116:149	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	6	9	theme	expression	1088:1097	arg1	level					1099:1103	the mRNA expression level	1079:1103	the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum	1079:1258	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	2	10	theme	gut	578:580	arg1	microbiota					582:591	gut microbiota	578:591	gut microbiota	578:591	The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota.
35689477	8	11	theme	increased	1463:1471	arg1	abundances					1486:1495	the relative abundances	1473:1495	the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota	1459:1549	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	1	12	theme	ferulic	219:225	arg1	ester					232:236	the ferulic acid ester	215:236	the ferulic acid ester of oligosaccharides	215:256	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	12	theme	ferulic	219:225	arg1	oligosaccharides					191:206	BACKGROUND Feruloyl oligosaccharides	171:206	BACKGROUND Feruloyl oligosaccharides (FOs)	171:212	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	8	13	theme	relative	1477:1484	arg1	abundances					1486:1495	the relative abundances	1473:1495	the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota	1459:1549	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	0	14	theme	gut	155:157	arg1	microbiota					159:168	gut microbiota	155:168	gut microbiota	155:168	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	4	15	theme	body	749:752	arg1	weight					754:759	100 mg kg-1 body weight	737:759	100 mg kg-1 body weight vitamin C	737:769	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	1	16	contain	possess	263:269	arg1	FOs					209:211	FOs	209:211	FOs	209:211	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	16	contain	possess	263:269	arg1	ester					232:236	the ferulic acid ester	215:236	the ferulic acid ester of oligosaccharides	215:256	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	16	contain	possess	263:269	arg2	activity					366:373	antioxidative activity	352:373	antioxidative activity	352:373	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	16	contain	possess	263:269	arg2	capacity					405:412	gut microbiota modulation capacity	379:412	gut microbiota modulation capacity	379:412	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	16	contain	possess	263:269	arg1	oligosaccharides					191:206	BACKGROUND Feruloyl oligosaccharides	171:206	BACKGROUND Feruloyl oligosaccharides (FOs)	171:212	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	16	contain	possess	263:269	arg2	functions					289:297	the physiological functions	271:297	the physiological functions	271:297	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	7	17	from	Increases	1261:1269	arg1	levels					1290:1295	phosphorylation levels	1274:1295	phosphorylation levels of Nrf2, p38 and JNK	1274:1316	Increases in phosphorylation levels of Nrf2, p38 and JNK were also observed.
35689477	4	18	theme	0.85	716:719	arg1	%					720:720	%	720:720	%	720:720	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	0	19	theme	intestinal	58:67	arg1	capacity					83:90	the intestinal antioxidative capacity	54:90	the intestinal antioxidative capacity of rats	54:98	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	10	20	theme	signaling	1911:1919	arg1	pathway					1921:1927	the p38/JNK-Nrf2 signaling pathway	1894:1927	the p38/JNK-Nrf2 signaling pathway	1894:1927	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	2	21	theme	rats	522:525	arg1	capacity					510:517	the intestinal antioxidative capacity	481:517	the intestinal antioxidative capacity of rats	481:525	The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota.
35689477	4	22	theme	normal	722:727	arg1	saline					729:734	0.85% normal saline	716:734	0.85% normal saline	716:734	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	1	23	theme	antioxidative	352:364	arg1	activity					366:373	antioxidative activity	352:373	antioxidative activity	352:373	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	5	24	theme	jejunum	905:911	arg1	capacity					889:896	the antioxidative capacity	871:896	the antioxidative capacity of the jejunum	871:911	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	0	25	from	Regulation	0:9	arg1	capacity					83:90	the intestinal antioxidative capacity	54:90	the intestinal antioxidative capacity of rats	54:98	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	0	26	theme	rats	95:98	arg1	capacity					83:90	the intestinal antioxidative capacity	54:90	the intestinal antioxidative capacity of rats	54:98	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	2	27	theme	MAPKs/Nrf2	545:554	arg1	pathway					566:572	the MAPKs/Nrf2 signaling pathway	541:572	the MAPKs/Nrf2 signaling pathway	541:572	The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota.
35689477	2	28	theme	present	419:425	arg1	study					427:431	The present study	415:431	The present study	415:431	The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota.
35689477	1	29	theme	microbiota	383:392	arg1	capacity					405:412	gut microbiota modulation capacity	379:412	gut microbiota modulation capacity	379:412	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	4	30	theme	vitamin	761:767	arg1	C					769:769	100 mg kg-1 body weight vitamin C	737:769	100 mg kg-1 body weight vitamin C	737:769	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	1	31	theme	physiological	275:287	arg1	capacity					405:412	gut microbiota modulation capacity	379:412	gut microbiota modulation capacity	379:412	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	31	theme	physiological	275:287	arg1	activity					366:373	antioxidative activity	352:373	antioxidative activity	352:373	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	31	theme	physiological	275:287	arg1	functions					289:297	the physiological functions	271:297	the physiological functions	271:297	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	4	32	theme	80 mg kg-1	810:819	arg1	weight					826:831	20, 40 and 80 mg kg-1 body weight	799:831	weight	826:831	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	0	33	theme	wheat	14:18	arg1	bran					20:23	wheat bran	14:23	wheat bran feruloyl oligosaccharides	14:49	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	8	34	theme	microbiota	1424:1433	arg1	composition					1399:1409	composition	1399:1409	composition	1399:1409	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	8	34	theme	microbiota	1424:1433	arg1	structure					1385:1393	structure	1385:1393	structure	1385:1393	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	0	35	theme	feruloyl	25:32	arg1	oligosaccharides					34:49	wheat bran feruloyl oligosaccharides	14:49	wheat bran feruloyl oligosaccharides	14:49	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	1	36	theme	ferulic	307:313	arg1	acid					315:318	ferulic acid	307:318	ferulic acid	307:318	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	2	37	theme	intestinal	485:494	arg1	capacity					510:517	the intestinal antioxidative capacity	481:517	the intestinal antioxidative capacity of rats	481:525	The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota.
35689477	8	38	theme	Lachnospiraceae_NK4A136_group	1609:1637	arg1	abundances					1583:1592	the relative abundances	1570:1592	the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia	1570:1649	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	4	39	theme	weight	826:831	arg1	doses					790:794	doses	790:794	doses of 20, 40 and 80 mg kg-1 body weight for 21 days	790:843	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	5	40	theme	glutathione	1025:1035	arg1	contents					943:950	contents	943:950	contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione	943:1035	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	6	41	theme	glutamate-cysteine	1135:1152	arg1	ligase					1154:1159	glutamate-cysteine ligase	1135:1159	glutamate-cysteine ligase catalytic subunit	1135:1177	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	8	42	theme	Blautia	1643:1649	arg1	abundances					1583:1592	the relative abundances	1570:1592	the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia	1570:1649	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	4	43	theme	20	799:800	arg1	weight					826:831	20, 40 and 80 mg kg-1 body weight	799:831	weight	826:831	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	1	44	theme	acid	227:230	arg1	ester					232:236	the ferulic acid ester	215:236	the ferulic acid ester of oligosaccharides	215:256	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	44	theme	acid	227:230	arg1	oligosaccharides					191:206	BACKGROUND Feruloyl oligosaccharides	171:206	BACKGROUND Feruloyl oligosaccharides (FOs)	171:212	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	5	45	theme	glutathione	990:1000	arg1	peroxidase					1002:1011	glutathione peroxidase	990:1011	glutathione peroxidase	990:1011	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	5	45	theme	glutathione	990:1000	arg1	catalase					955:962	catalase	955:962	catalase	955:962	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	6	46	theme	genes	1128:1132	arg1	level					1099:1103	the mRNA expression level	1079:1103	the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum	1079:1258	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	11	47	theme	Chemical	1967:1974	arg1	Industry					1976:1983	Chemical Industry	1967:1983	Chemical Industry	1967:1983	© 2022 Society of Chemical Industry.
35689477	6	48	theme	catalytic	1161:1169	arg1	subunit					1171:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	48	theme	catalytic	1161:1169	arg1	genes					1128:1132	antioxidant-related genes	1108:1132	antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1)	1108:1243	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	9	49	theme	cecal	1721:1725	arg1	microbiota					1727:1736	the altered cecal microbiota	1709:1736	the altered cecal microbiota	1709:1736	Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats.
35689477	7	50	theme	phosphorylation	1274:1288	arg1	levels					1290:1295	phosphorylation levels	1274:1295	phosphorylation levels of Nrf2, p38 and JNK	1274:1316	Increases in phosphorylation levels of Nrf2, p38 and JNK were also observed.
35689477	8	51	theme	40 mg kg-1	1358:1367	arg1	FOs					1369:1371	40 mg kg-1 FOs	1358:1371	40 mg kg-1 FOs	1358:1371	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	7	52	theme	p38	1306:1308	arg1	levels					1290:1295	phosphorylation levels	1274:1295	phosphorylation levels of Nrf2, p38 and JNK	1274:1316	Increases in phosphorylation levels of Nrf2, p38 and JNK were also observed.
35689477	1	53	theme	Feruloyl	182:189	arg1	FOs					209:211	FOs	209:211	FOs	209:211	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	53	theme	Feruloyl	182:189	arg1	oligosaccharides					191:206	BACKGROUND Feruloyl oligosaccharides	171:206	BACKGROUND Feruloyl oligosaccharides (FOs)	171:212	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	53	theme	Feruloyl	182:189	arg1	ester					232:236	the ferulic acid ester	215:236	the ferulic acid ester of oligosaccharides	215:256	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	6	54	theme	ligase	1199:1204	arg1	subunit					1215:1221	glutamate-cysteine ligase modifier subunit	1180:1221	glutamate-cysteine ligase modifier subunit	1180:1221	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	54	theme	ligase	1199:1204	arg1	genes					1128:1132	antioxidant-related genes	1108:1132	antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1)	1108:1243	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	9	55	theme	antioxidative	1770:1782	arg1	capacity					1784:1791	jejunal antioxidative capacity	1762:1791	jejunal antioxidative capacity of rats	1762:1799	Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats.
35689477	0	56	theme	signaling	133:141	arg1	pathway					143:149	the p38/JNK-Nrf2 signaling pathway	116:149	the p38/JNK-Nrf2 signaling pathway	116:149	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	9	57	theme	correlation	1674:1684	arg1	analysis					1686:1693	Spearman correlation analysis	1665:1693	Spearman correlation analysis	1665:1693	Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats.
35689477	10	58	theme	gut	1853:1855	arg1	improvement					1863:1873	gut heath improvement	1853:1873	gut heath improvement	1853:1873	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	8	59	dep	structure	1385:1393	arg1	the					1381:1383	the	1381:1383	the	1381:1383	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	1	60	theme	BACKGROUND	171:180	arg1	FOs					209:211	FOs	209:211	FOs	209:211	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	60	theme	BACKGROUND	171:180	arg1	oligosaccharides					191:206	BACKGROUND Feruloyl oligosaccharides	171:206	BACKGROUND Feruloyl oligosaccharides (FOs)	171:212	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	60	theme	BACKGROUND	171:180	arg1	ester					232:236	the ferulic acid ester	215:236	the ferulic acid ester of oligosaccharides	215:256	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	10	61	theme	p38/JNK-Nrf2	1898:1909	arg1	pathway					1921:1927	the p38/JNK-Nrf2 signaling pathway	1894:1927	the p38/JNK-Nrf2 signaling pathway	1894:1927	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	8	62	theme	Actinobacteria	1500:1513	arg1	abundances					1486:1495	the relative abundances	1473:1495	the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota	1459:1549	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	10	63	theme	gut	1933:1935	arg1	microbiota					1937:1946	gut microbiota	1933:1946	gut microbiota	1933:1946	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	8	64	theme	Proteobacteria	1516:1529	arg1	abundances					1486:1495	the relative abundances	1473:1495	the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota	1459:1549	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	4	65	theme	100 mg kg-1	737:747	arg1	weight					754:759	100 mg kg-1 body weight	737:759	100 mg kg-1 body weight vitamin C	737:769	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	0	66	theme	antioxidative	69:81	arg1	capacity					83:90	the intestinal antioxidative capacity	54:90	the intestinal antioxidative capacity of rats	54:98	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	10	67	used	used	1826:1829	arg2	FOs					1813:1815	CONCLUSION FOs	1802:1815	CONCLUSION FOs	1802:1815	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	10	67	used	used	1826:1829	arg2	antioxidant					1837:1847	an antioxidant	1834:1847	an antioxidant for gut heath improvement	1834:1873	CONCLUSION FOs could be used as an antioxidant for gut heath improvement through modulating the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	8	68	theme	Acidobacteriota	1535:1549	arg1	abundances					1486:1495	the relative abundances	1473:1495	the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota	1459:1549	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	6	69	theme	heme	1227:1230	arg1	oxygenase-1					1232:1242	heme oxygenase-1	1227:1242	heme oxygenase-1	1227:1242	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	69	theme	heme	1227:1230	arg1	genes					1128:1132	antioxidant-related genes	1108:1132	antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1)	1108:1243	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	4	70	theme	%	720:720	arg1	saline					729:734	0.85% normal saline	716:734	0.85% normal saline	716:734	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	6	71	dep	genes	1128:1132	arg1	subunit					1215:1221	glutamate-cysteine ligase modifier subunit	1180:1221	glutamate-cysteine ligase modifier subunit	1180:1221	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	71	dep	genes	1128:1132	arg1	subunit					1171:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	71	dep	genes	1128:1132	arg1	genes					1128:1132	antioxidant-related genes	1108:1132	antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1)	1108:1243	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	71	dep	genes	1128:1132	arg1	oxygenase-1					1232:1242	heme oxygenase-1	1227:1242	heme oxygenase-1	1227:1242	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	5	72	theme	catalase	955:962	arg1	contents					943:950	contents	943:950	contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione	943:1035	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	3	73	theme	Wistar	601:606	arg1	rats					608:611	Thirty Wistar rats	594:611	Thirty Wistar rats	594:611	Thirty Wistar rats were randomly divided into five groups.
35689477	2	74	theme	signaling	556:564	arg1	pathway					566:572	the MAPKs/Nrf2 signaling pathway	541:572	the MAPKs/Nrf2 signaling pathway	541:572	The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota.
35689477	4	75	theme	FOs	774:776	arg1	solution					778:785	FOs solution	774:785	FOs solution	774:785	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	1	76	theme	gut	379:381	arg1	capacity					405:412	gut microbiota modulation capacity	379:412	gut microbiota modulation capacity	379:412	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	5	77	theme	superoxide	965:974	arg1	catalase					955:962	catalase	955:962	catalase	955:962	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	5	77	theme	superoxide	965:974	arg1	dismutase					976:984	superoxide dismutase	965:984	superoxide dismutase	965:984	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	6	78	theme	mRNA	1083:1086	arg1	level					1099:1103	the mRNA expression level	1079:1103	the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum	1079:1258	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	8	79	theme	relative	1574:1581	arg1	abundances					1583:1592	the relative abundances	1570:1592	the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia	1570:1649	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	1	80	theme	modulation	394:403	arg1	capacity					405:412	gut microbiota modulation capacity	379:412	gut microbiota modulation capacity	379:412	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	9	81	theme	rats	1796:1799	arg1	capacity					1784:1791	jejunal antioxidative capacity	1762:1791	jejunal antioxidative capacity of rats	1762:1799	Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats.
35689477	4	82	theme	weight	754:759	arg1	C					769:769	100 mg kg-1 body weight vitamin C	737:769	100 mg kg-1 body weight vitamin C	737:769	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	0	83	theme	bran	20:23	arg1	oligosaccharides					34:49	wheat bran feruloyl oligosaccharides	14:49	wheat bran feruloyl oligosaccharides	14:49	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	6	84	theme	FOs	1048:1050	arg1	administration					1052:1065	FOs administration	1048:1065	FOs administration	1048:1065	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	0	85	theme	oligosaccharides	34:49	arg1	Regulation					0:9	Regulation	0:9	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats	0:98	Regulation of wheat bran feruloyl oligosaccharides in the intestinal antioxidative capacity of rats associated with the p38/JNK-Nrf2 signaling pathway and gut microbiota.
35689477	7	86	theme	JNK	1314:1316	arg1	levels					1290:1295	phosphorylation levels	1274:1295	phosphorylation levels of Nrf2, p38 and JNK	1274:1316	Increases in phosphorylation levels of Nrf2, p38 and JNK were also observed.
35689477	2	87	theme	antioxidative	496:508	arg1	capacity					510:517	the intestinal antioxidative capacity	481:517	the intestinal antioxidative capacity of rats	481:525	The present study aimed to investigate whether FOs could regulate the intestinal antioxidative capacity of rats by modulating the MAPKs/Nrf2 signaling pathway and gut microbiota.
35689477	8	88	theme	cecal	1418:1422	arg1	microbiota					1424:1433	the cecal microbiota	1414:1433	the cecal microbiota	1414:1433	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	1	89	theme	acid	315:318	arg1	capacity					405:412	gut microbiota modulation capacity	379:412	gut microbiota modulation capacity	379:412	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	89	theme	acid	315:318	arg1	activity					366:373	antioxidative activity	352:373	antioxidative activity	352:373	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	89	theme	acid	315:318	arg1	functions					289:297	the physiological functions	271:297	the physiological functions	271:297	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	9	90	theme	Spearman	1665:1672	arg1	analysis					1686:1693	Spearman correlation analysis	1665:1693	Spearman correlation analysis	1665:1693	Furthermore, Spearman correlation analysis revealed that the altered cecal microbiota closely correlated with jejunal antioxidative capacity of rats.
35689477	6	91	theme	ligase	1154:1159	arg1	subunit					1171:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	91	theme	ligase	1154:1159	arg1	genes					1128:1132	antioxidant-related genes	1108:1132	antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1)	1108:1243	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	4	92	theme	body	821:824	arg1	weight					826:831	20, 40 and 80 mg kg-1 body weight	799:831	weight	826:831	Rats received a standard diet and were gavaged once daily with 0.85% normal saline, 100 mg kg-1 body weight vitamin C or FOs solution at doses of 20, 40 and 80 mg kg-1 body weight for 21 days.
35689477	1	93	theme	oligosaccharides	324:339	arg1	capacity					405:412	gut microbiota modulation capacity	379:412	gut microbiota modulation capacity	379:412	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	93	theme	oligosaccharides	324:339	arg1	activity					366:373	antioxidative activity	352:373	antioxidative activity	352:373	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	93	theme	oligosaccharides	324:339	arg1	functions					289:297	the physiological functions	271:297	the physiological functions	271:297	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	7	94	theme	Nrf2	1300:1303	arg1	levels					1290:1295	phosphorylation levels	1274:1295	phosphorylation levels of Nrf2, p38 and JNK	1274:1316	Increases in phosphorylation levels of Nrf2, p38 and JNK were also observed.
35689477	8	95	with	Administration	1338:1351	arg1	FOs					1369:1371	40 mg kg-1 FOs	1358:1371	40 mg kg-1 FOs	1358:1371	Administration with 40 mg kg-1 FOs altered the structure and composition of the cecal microbiota, which was indicated by the increased the relative abundances of Actinobacteria, Proteobacteria and Acidobacteriota, and the decreased the relative abundances of Firmicutes, Lachnospiraceae_NK4A136_group and Blautia.
35689477	5	96	dep	RESULTS	846:852	arg1	FOs					854:856	FOs	854:856	RESULTS FOs	846:856	RESULTS FOs strengthened the antioxidative capacity of the jejunum, as indicated by increased in contents of catalase, superoxide dismutase and glutathione peroxidase, as well as glutathione.
35689477	6	97	from	level	1099:1103	arg1	jejunum					1252:1258	the jejunum	1248:1258	the jejunum	1248:1258	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	98	theme	antioxidant-related	1108:1126	arg1	subunit					1215:1221	glutamate-cysteine ligase modifier subunit	1180:1221	glutamate-cysteine ligase modifier subunit	1180:1221	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	98	theme	antioxidant-related	1108:1126	arg1	subunit					1171:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	glutamate-cysteine ligase catalytic subunit	1135:1177	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	98	theme	antioxidant-related	1108:1126	arg1	genes					1128:1132	antioxidant-related genes	1108:1132	antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1)	1108:1243	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	6	98	theme	antioxidant-related	1108:1126	arg1	oxygenase-1					1232:1242	heme oxygenase-1	1227:1242	heme oxygenase-1	1227:1242	Moreover, FOs administration upregulated the mRNA expression level of antioxidant-related genes (glutamate-cysteine ligase catalytic subunit, glutamate-cysteine ligase modifier subunit and heme oxygenase-1) in the jejunum.
35689477	1	99	theme	oligosaccharides	241:256	arg1	ester					232:236	the ferulic acid ester	215:236	the ferulic acid ester of oligosaccharides	215:256	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
35689477	1	99	theme	oligosaccharides	241:256	arg1	oligosaccharides					191:206	BACKGROUND Feruloyl oligosaccharides	171:206	BACKGROUND Feruloyl oligosaccharides (FOs)	171:212	BACKGROUND Feruloyl oligosaccharides (FOs), the ferulic acid ester of oligosaccharides, may possess the physiological functions of both ferulic acid and oligosaccharides, including antioxidative activity and gut microbiota modulation capacity.
34964882	13	0	dep	CONCLUSIONS	1825:1835	arg1	kill					1876:1879	kill	1876:1879	kill	1876:1879	CONCLUSIONS Gut epithelial cells-derived CXCL9 can kill the expanded E. coli through aceE, to remain gut homeostasis.
34964882	8	1	theme	colonic	1362:1368	arg1	contents					1370:1377	the colonic contents	1358:1377	the colonic contents of CXCL9fl/flpvillin-creT mice	1358:1408	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	2	2	contain	possess	236:242	arg2	activity					268:275	potential antimicrobial activity	244:275	potential antimicrobial activity	244:275	Chemokines are shown to possess potential antimicrobial activity, including against Gram-positive and -negative bacterial pathogens.
34964882	2	2	contain	possess	236:242	arg1	Chemokines					212:221	Chemokines	212:221	Chemokines	212:221	Chemokines are shown to possess potential antimicrobial activity, including against Gram-positive and -negative bacterial pathogens.
34964882	4	3	theme	CXCL9fl/flpvillin-creT	509:530	arg1	mice					532:535	CXCL9fl/flpvillin-creT mice	509:535	CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice]	509:563	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	13	4	theme	cells-derived	1852:1864	arg1	CXCL9					1866:1870	Gut epithelial cells-derived CXCL9	1837:1870	Gut epithelial cells-derived CXCL9	1837:1870	CONCLUSIONS Gut epithelial cells-derived CXCL9 can kill the expanded E. coli through aceE, to remain gut homeostasis.
34964882	4	5	theme	CXCL9fl/flpvillin-crewmice	583:608	arg1	mice					632:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	13	6	theme	epithelial	1841:1850	arg1	CXCL9					1866:1870	Gut epithelial cells-derived CXCL9	1837:1870	Gut epithelial cells-derived CXCL9	1837:1870	CONCLUSIONS Gut epithelial cells-derived CXCL9 can kill the expanded E. coli through aceE, to remain gut homeostasis.
34964882	4	7	dep	mice	532:535	arg1	mice					559:562	positive mice	550:562	positive mice	550:562	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	2	8	theme	Gram-positive	296:308	arg1	pathogens					334:342	Gram-positive and -negative bacterial pathogens	296:342	Gram-positive and -negative bacterial pathogens	296:342	Chemokines are shown to possess potential antimicrobial activity, including against Gram-positive and -negative bacterial pathogens.
34964882	6	9	dep	E.	961:962	arg1	coli					964:967	coli	964:967	coli	964:967	We generated E. coli ΔFtsX [FtsX-depleted E. coli] and E. coli ΔaceE [aceE-depleted E. coli] by using a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli.
34964882	3	10	theme	chemokine	381:389	arg1	CXCL9					391:395	chemokine CXCL9	381:395	chemokine CXCL9 expressed in the gut epithelial cells	381:433	We here investigated function[s] of chemokine CXCL9 expressed in the gut epithelial cells, and mechanism[s] of CXCL9 by which to kill E. coli.
34964882	0	11	link	Epithelial-derived	4:21	arg1	CXCL9					23:27	Gut Epithelial-derived CXCL9	0:27	Gut Epithelial-derived CXCL9	0:27	Gut Epithelial-derived CXCL9 Maintains Gut Homeostasis Through Preventing Overgrown E. coli.
34964882	9	12	theme	epithelial	1582:1591	arg1	cells					1593:1597	gut epithelial cells	1578:1597	gut epithelial cells	1578:1597	The increased E. coli could promote colitis through NLRC4 and caspase 1/11-mediated IL-18, which was derived from gut epithelial cells.
34964882	4	13	theme	negative	623:630	arg1	mice					632:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	11	14	theme	PDHc	1730:1733	arg1	subunits					1735:1742	Ftsx and PDHc subunits	1721:1742	Ftsx and PDHc subunits	1721:1742	E. coli expressed Ftsx and PDHc subunits aceE.
34964882	5	15	theme	region	866:871	arg1	sequencing					874:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	6	16	theme	recombining	1037:1047	arg1	system					1049:1054	a bacterium red recombining system	1021:1054	a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli	1021:1120	We generated E. coli ΔFtsX [FtsX-depleted E. coli] and E. coli ΔaceE [aceE-depleted E. coli] by using a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli.
34964882	5	17	theme	metagenomic	897:907	arg1	analyses					909:916	shotgun metagenomic analyses	889:916	shotgun metagenomic analyses	889:916	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	6	18	theme	CXCL9	1091:1095	arg1	mechanism[s					1075:1085	the mechanism[s]	1071:1086	the mechanism[s] of CXCL9 by which to kill E. coli	1071:1120	We generated E. coli ΔFtsX [FtsX-depleted E. coli] and E. coli ΔaceE [aceE-depleted E. coli] by using a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli.
34964882	13	19	theme	Gut	1837:1839	arg1	CXCL9					1866:1870	Gut epithelial cells-derived CXCL9	1837:1870	Gut epithelial cells-derived CXCL9	1837:1870	CONCLUSIONS Gut epithelial cells-derived CXCL9 can kill the expanded E. coli through aceE, to remain gut homeostasis.
34964882	4	20	theme	[pvillin-cre	610:621	arg1	mice					632:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	5	21	theme	ribosomal	836:844	arg1	sequencing					874:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	13	22	theme	gut	1926:1928	arg1	homeostasis					1930:1940	gut homeostasis	1926:1940	gut homeostasis	1926:1940	CONCLUSIONS Gut epithelial cells-derived CXCL9 can kill the expanded E. coli through aceE, to remain gut homeostasis.
34964882	5	23	theme	shotgun	889:895	arg1	analyses					909:916	shotgun metagenomic analyses	889:916	shotgun metagenomic analyses	889:916	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	0	24	theme	Epithelial-derived	4:21	arg1	CXCL9					23:27	Gut Epithelial-derived CXCL9	0:27	Gut Epithelial-derived CXCL9	0:27	Gut Epithelial-derived CXCL9 Maintains Gut Homeostasis Through Preventing Overgrown E. coli.
34964882	11	25	theme	Ftsx	1721:1724	arg1	subunits					1735:1742	Ftsx and PDHc subunits	1721:1742	Ftsx and PDHc subunits	1721:1742	E. coli expressed Ftsx and PDHc subunits aceE.
34964882	10	26	theme	epithelial	1652:1661	arg1	cells					1663:1667	gut epithelial cells	1648:1667	gut epithelial cells	1648:1667	We finally demonstrated that CXCL9 expressed in gut epithelial cells could kill the overgrown E. coli.
34964882	4	27	theme	-mediated	688:696	arg1	model					706:710	a dextran sulphate sodium [DSS]-mediated colitis model	657:710	a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice	657:770	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	0	28	theme	Gut	0:2	arg1	CXCL9					23:27	Gut Epithelial-derived CXCL9	0:27	Gut Epithelial-derived CXCL9	0:27	Gut Epithelial-derived CXCL9 Maintains Gut Homeostasis Through Preventing Overgrown E. coli.
34964882	8	29	theme	DSS	1275:1277	arg1	treatment					1279:1287	DSS treatment	1275:1287	DSS treatment	1275:1287	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	6	30	dep	coli	935:938	arg1	E.					1003:1004	E.	1003:1004	E.	1003:1004	We generated E. coli ΔFtsX [FtsX-depleted E. coli] and E. coli ΔaceE [aceE-depleted E. coli] by using a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli.
34964882	2	31	theme	bacterial	324:332	arg1	pathogens					334:342	Gram-positive and -negative bacterial pathogens	296:342	Gram-positive and -negative bacterial pathogens	296:342	Chemokines are shown to possess potential antimicrobial activity, including against Gram-positive and -negative bacterial pathogens.
34964882	9	32	theme	gut	1578:1580	arg1	cells					1593:1597	gut epithelial cells	1578:1597	gut epithelial cells	1578:1597	The increased E. coli could promote colitis through NLRC4 and caspase 1/11-mediated IL-18, which was derived from gut epithelial cells.
34964882	3	33	theme	CXCL9	391:395	arg1	mechanism[s					440:450	mechanism[s]	440:451	mechanism[s] of CXCL9 by which to kill E. coli	440:485	We here investigated function[s] of chemokine CXCL9 expressed in the gut epithelial cells, and mechanism[s] of CXCL9 by which to kill E. coli.
34964882	3	33	theme	CXCL9	391:395	arg1	function[s					366:375	function[s]	366:376	function[s] of chemokine CXCL9 expressed in the gut epithelial cells	366:433	We here investigated function[s] of chemokine CXCL9 expressed in the gut epithelial cells, and mechanism[s] of CXCL9 by which to kill E. coli.
34964882	8	34	dep	E.	1324:1325	arg1	coli					1327:1330	coli	1327:1330	coli	1327:1330	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	2	35	theme	-negative	314:322	arg1	pathogens					334:342	Gram-positive and -negative bacterial pathogens	296:342	Gram-positive and -negative bacterial pathogens	296:342	Chemokines are shown to possess potential antimicrobial activity, including against Gram-positive and -negative bacterial pathogens.
34964882	5	36	theme	variable	857:864	arg1	sequencing					874:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	4	37	theme	mice	767:770	arg1	sensitivity					729:739	the sensitivity	725:739	the sensitivity of CXCL9fl/flpvillin-creT mice	725:770	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	4	38	theme	control	575:581	arg1	mice					632:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice	569:635	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	8	39	theme	mice	1405:1408	arg1	contents					1370:1377	the colonic contents	1358:1377	the colonic contents of CXCL9fl/flpvillin-creT mice	1358:1408	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	1	40	theme	multiple	193:200	arg1	diseases					202:209	multiple diseases	193:209	multiple diseases	193:209	BACKGROUND AND AIMS Increased E. coli in the colon are related to the occurrence and development of multiple diseases.
34964882	5	41	theme	gut	808:810	arg1	microbiota					812:821	the gut microbiota	804:821	the gut microbiota	804:821	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	9	42	attach	derived	1565:1571	arg2	IL-18					1548:1552	caspase 1/11-mediated IL-18	1526:1552	caspase 1/11-mediated IL-18	1526:1552	The increased E. coli could promote colitis through NLRC4 and caspase 1/11-mediated IL-18, which was derived from gut epithelial cells.
34964882	9	42	attach	derived	1565:1571	arg2	NLRC4					1516:1520	NLRC4	1516:1520	NLRC4	1516:1520	The increased E. coli could promote colitis through NLRC4 and caspase 1/11-mediated IL-18, which was derived from gut epithelial cells.
34964882	9	42	attach	derived	1565:1571	arg1	cells					1593:1597	gut epithelial cells	1578:1597	gut epithelial cells	1578:1597	The increased E. coli could promote colitis through NLRC4 and caspase 1/11-mediated IL-18, which was derived from gut epithelial cells.
34964882	1	43	theme	diseases	202:209	arg1	development					178:188	development	178:188	development	178:188	BACKGROUND AND AIMS Increased E. coli in the colon are related to the occurrence and development of multiple diseases.
34964882	1	43	theme	diseases	202:209	arg1	occurrence					163:172	occurrence	163:172	occurrence	163:172	BACKGROUND AND AIMS Increased E. coli in the colon are related to the occurrence and development of multiple diseases.
34964882	3	44	theme	CXCL9	456:460	arg1	mechanism[s					440:450	mechanism[s]	440:451	mechanism[s] of CXCL9 by which to kill E. coli	440:485	We here investigated function[s] of chemokine CXCL9 expressed in the gut epithelial cells, and mechanism[s] of CXCL9 by which to kill E. coli.
34964882	3	44	theme	CXCL9	456:460	arg1	function[s					366:375	function[s]	366:376	function[s] of chemokine CXCL9 expressed in the gut epithelial cells	366:433	We here investigated function[s] of chemokine CXCL9 expressed in the gut epithelial cells, and mechanism[s] of CXCL9 by which to kill E. coli.
34964882	0	45	theme	Gut	39:41	arg1	Homeostasis					43:53	Gut Homeostasis	39:53	Gut Homeostasis	39:53	Gut Epithelial-derived CXCL9 Maintains Gut Homeostasis Through Preventing Overgrown E. coli.
34964882	5	46	theme	microbiota	812:821	arg1	composition					789:799	the composition	785:799	the composition of the gut microbiota	785:821	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	13	47	link	cells-derived	1852:1864	arg1	CXCL9					1866:1870	Gut epithelial cells-derived CXCL9	1837:1870	Gut epithelial cells-derived CXCL9	1837:1870	CONCLUSIONS Gut epithelial cells-derived CXCL9 can kill the expanded E. coli through aceE, to remain gut homeostasis.
34964882	5	48	theme	RNA	846:848	arg1	sequencing					874:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	4	49	theme	dextran	659:665	arg1	model					706:710	a dextran sulphate sodium [DSS]-mediated colitis model	657:710	a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice	657:770	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	3	50	theme	gut	414:416	arg1	cells					429:433	the gut epithelial cells	410:433	the gut epithelial cells	410:433	We here investigated function[s] of chemokine CXCL9 expressed in the gut epithelial cells, and mechanism[s] of CXCL9 by which to kill E. coli.
34964882	9	51	theme	1/11-mediated	1534:1546	arg1	IL-18					1548:1552	caspase 1/11-mediated IL-18	1526:1552	caspase 1/11-mediated IL-18	1526:1552	The increased E. coli could promote colitis through NLRC4 and caspase 1/11-mediated IL-18, which was derived from gut epithelial cells.
34964882	7	52	dep	RESULTS	1123:1129	arg1	sensitive					1169:1177	sensitive	1169:1177	sensitive	1169:1177	RESULTS CXCL9 fl/flpvillin-creTmice were more sensitive to chemically induced colitis than their control littermates, CXCL9fl/flpvillin-crewmice.
34964882	8	53	theme	E.	1324:1325	arg1	[Escherichia-Shigella					1332:1352	gut E. coli [Escherichia-Shigella]	1320:1353	gut E. coli [Escherichia-Shigella]	1320:1353	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	3	54	theme	epithelial	418:427	arg1	cells					429:433	the gut epithelial cells	410:433	the gut epithelial cells	410:433	We here investigated function[s] of chemokine CXCL9 expressed in the gut epithelial cells, and mechanism[s] of CXCL9 by which to kill E. coli.
34964882	1	55	dep	occurrence	163:172	arg1	the					159:161	the	159:161	the	159:161	BACKGROUND AND AIMS Increased E. coli in the colon are related to the occurrence and development of multiple diseases.
34964882	9	56	theme	caspase	1526:1532	arg1	IL-18					1548:1552	caspase 1/11-mediated IL-18	1526:1552	caspase 1/11-mediated IL-18	1526:1552	The increased E. coli could promote colitis through NLRC4 and caspase 1/11-mediated IL-18, which was derived from gut epithelial cells.
34964882	8	57	theme	gut	1320:1322	arg1	[Escherichia-Shigella					1332:1352	gut E. coli [Escherichia-Shigella]	1320:1353	gut E. coli [Escherichia-Shigella]	1320:1353	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	4	58	theme	positive	550:557	arg1	mice					559:562	positive mice	550:562	positive mice	550:562	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	8	59	theme	CXCL9fl/flpvillin-crew	1435:1456	arg1	mice					1458:1461	control CXCL9fl/flpvillin-crew mice	1427:1461	control CXCL9fl/flpvillin-crew mice	1427:1461	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	4	60	theme	CXCL9fl/flpvillin-creT	744:765	arg1	mice					767:770	CXCL9fl/flpvillin-creT mice	744:770	CXCL9fl/flpvillin-creT mice	744:770	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	8	61	theme	control	1427:1433	arg1	mice					1458:1461	control CXCL9fl/flpvillin-crew mice	1427:1461	control CXCL9fl/flpvillin-crew mice	1427:1461	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	5	62	theme	16S	832:834	arg1	sequencing					874:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	8	63	theme	CXCL9fl/flpvillin-creT	1382:1403	arg1	mice					1405:1408	CXCL9fl/flpvillin-creT mice	1382:1408	CXCL9fl/flpvillin-creT mice	1382:1408	After DSS treatment, there were markedly increased gut E. coli [Escherichia-Shigella] in the colonic contents of CXCL9fl/flpvillin-creT mice as compared with control CXCL9fl/flpvillin-crew mice.
34964882	6	64	dep	E.	1003:1004	arg1	coli					1006:1009	coli	1006:1009	coli	1006:1009	We generated E. coli ΔFtsX [FtsX-depleted E. coli] and E. coli ΔaceE [aceE-depleted E. coli] by using a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli.
34964882	2	65	theme	antimicrobial	254:266	arg1	activity					268:275	potential antimicrobial activity	244:275	potential antimicrobial activity	244:275	Chemokines are shown to possess potential antimicrobial activity, including against Gram-positive and -negative bacterial pathogens.
34964882	4	66	dep	METHODS	488:494	arg1	employed					648:655	employed	648:655	employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice	648:770	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	4	66	dep	METHODS	488:494	arg1	generated					499:507	generated	499:507	generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice]	499:636	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	6	67	theme	red	1033:1035	arg1	system					1049:1054	a bacterium red recombining system	1021:1054	a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli	1021:1120	We generated E. coli ΔFtsX [FtsX-depleted E. coli] and E. coli ΔaceE [aceE-depleted E. coli] by using a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli.
34964882	2	68	theme	potential	244:252	arg1	activity					268:275	potential antimicrobial activity	244:275	potential antimicrobial activity	244:275	Chemokines are shown to possess potential antimicrobial activity, including against Gram-positive and -negative bacterial pathogens.
34964882	12	69	theme	CXCL9-mediated	1801:1814	arg1	killing					1816:1822	CXCL9-mediated killing	1801:1822	CXCL9-mediated killing	1801:1822	E.coliΔaceE but not E. coliΔFtsX were resistant to CXCL9-mediated killing.
34964882	6	70	theme	bacterium	1023:1031	arg1	system					1049:1054	a bacterium red recombining system	1021:1054	a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli	1021:1120	We generated E. coli ΔFtsX [FtsX-depleted E. coli] and E. coli ΔaceE [aceE-depleted E. coli] by using a bacterium red recombining system to investigate the mechanism[s] of CXCL9 by which to kill E. coli.
34964882	7	71	theme	CXCL9	1131:1135	arg1	fl/flpvillin-creTmice					1137:1157	CXCL9 fl/flpvillin-creTmice	1131:1157	CXCL9 fl/flpvillin-creTmice	1131:1157	RESULTS CXCL9 fl/flpvillin-creTmice were more sensitive to chemically induced colitis than their control littermates, CXCL9fl/flpvillin-crewmice.
34964882	4	72	dep	mice	559:562	arg1	[pvillin-cre					537:548	[pvillin-cre	537:548	[pvillin-cre	537:548	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	5	73	theme	[V3-V4	850:855	arg1	sequencing					874:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	16S ribosomal RNA [V3-V4 variable region] sequencing	832:883	We analysed the composition of the gut microbiota by using 16S ribosomal RNA [V3-V4 variable region] sequencing and shotgun metagenomic analyses.
34964882	10	74	theme	gut	1648:1650	arg1	cells					1663:1667	gut epithelial cells	1648:1667	gut epithelial cells	1648:1667	We finally demonstrated that CXCL9 expressed in gut epithelial cells could kill the overgrown E. coli.
34964882	4	75	theme	colitis	698:704	arg1	model					706:710	a dextran sulphate sodium [DSS]-mediated colitis model	657:710	a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice	657:770	METHODS We generated CXCL9fl/flpvillin-creT mice [pvillin-cre positive mice] and their control CXCL9fl/flpvillin-crewmice [pvillin-cre negative mice], and then employed a dextran sulphate sodium [DSS]-mediated colitis model to determine the sensitivity of CXCL9fl/flpvillin-creT mice.
34964882	7	76	theme	induced	1193:1199	arg1	colitis					1201:1207	chemically induced colitis	1182:1207	chemically induced colitis	1182:1207	RESULTS CXCL9 fl/flpvillin-creTmice were more sensitive to chemically induced colitis than their control littermates, CXCL9fl/flpvillin-crewmice.
34964882	7	77	theme	control	1220:1226	arg1	littermates					1228:1238	their control littermates	1214:1238	their control littermates	1214:1238	RESULTS CXCL9 fl/flpvillin-creTmice were more sensitive to chemically induced colitis than their control littermates, CXCL9fl/flpvillin-crewmice.
36640951	12	0	theme	self-healing	2134:2145	arg1	capabilities					2178:2189	self-healing and pH-responsive drug release capabilities	2134:2189	self-healing and pH-responsive drug release capabilities	2134:2189	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	6	1	theme	endowed	953:959	arg1	gel					966:968	endowed CQCS@gel	953:968	endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	953:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	7	2	theme	gel	1263:1265	arg1	rate					1219:1222	rapid gelation rate	1204:1222	rapid gelation rate	1204:1222	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	7	2	theme	gel	1263:1265	arg1	tissue-adhesiveness					1235:1253	strong tissue-adhesiveness	1228:1253	strong tissue-adhesiveness	1228:1253	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	10	3	theme	quaternized	1778:1788	arg1	chitosan					1790:1797	catechol-functionalized quaternized chitosan	1754:1797	catechol-functionalized quaternized chitosan	1754:1797	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	3	4	theme	wound	536:540	arg1	healing					542:548	the hemostatic and wound healing	517:548	healing	542:548	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	7	5	dep	in	1076:1077	arg1	vivo					1079:1082	vivo	1079:1082	vivo	1079:1082	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	14	6	theme	skin	2516:2519	arg1	wounds					2521:2526	infected skin wounds	2507:2526	infected skin wounds	2507:2526	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	3	7	theme	hemostatic	521:530	arg1	healing					542:548	the hemostatic and wound healing	517:548	healing	542:548	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	9	8	theme	multifunctional	1463:1477	arg1	hydrogel					1490:1497	this multifunctional injectable hydrogel	1458:1497	this multifunctional injectable hydrogel	1458:1497	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	12	9	theme	CQCS	2120:2123	arg1	gel					2125:2127	the CQCS@gel	2116:2127	the CQCS@gel	2116:2127	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	11	10	theme	antioxidant	2042:2052	arg1	properties					2054:2063	its tissue adhesive and antioxidant properties	2018:2063	its tissue adhesive and antioxidant properties	2018:2063	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	14	11	theme	In	2311:2312	arg1	results					2332:2338	In vitro and in vivo results	2311:2338	In vitro and in vivo results	2311:2338	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	6	12	with	gel	966:968	arg1	properties					1051:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	11	13	theme	ammonium	1884:1891	arg1	groups					1893:1898	quaternary ammonium groups	1873:1898	quaternary ammonium groups	1873:1898	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	7	14	theme	acute	1123:1127	arg1	injuries					1136:1143	acute tissue injuries	1123:1143	acute tissue injuries such as liver and carotid wounds	1123:1176	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	7	14	theme	acute	1123:1127	arg1	liver					1153:1157	liver	1153:1157	liver	1153:1157	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	7	14	theme	acute	1123:1127	arg1	wounds					1171:1176	carotid wounds	1163:1176	carotid wounds	1163:1176	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	14	15	theme	accelerated	2484:2494	arg1	healing					2496:2502	accelerated healing	2484:2502	accelerated healing of infected skin wounds	2484:2526	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	1	16	theme	each	224:227	arg1	year					229:232	people each year	217:232	people each year	217:232	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	9	17	theme	injectable	1479:1488	arg1	hydrogel					1490:1497	this multifunctional injectable hydrogel	1458:1497	this multifunctional injectable hydrogel	1458:1497	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	8	18	theme	@	1282:1282	arg1	gel					1283:1285	CQCS@gel	1278:1285	CQCS@gel	1278:1285	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	5	19	theme	injectable	750:759	arg1	gel					776:778	CQCS@gel	771:778	CQCS@gel	771:778	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	19	theme	injectable	750:759	arg1	hydrogel					761:768	a bioinspired multifunctional injectable hydrogel	720:768	a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000)	720:930	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	11	20	theme	killing	1964:1970	arg1	efficiency					1972:1981	outstanding contact-active bacterial killing efficiency	1927:1981	outstanding contact-active bacterial killing efficiency	1927:1981	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	5	21	theme	dibenzaldehyde-terminated	871:895	arg1	poly					897:900	dibenzaldehyde-terminated poly	871:900	dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000)	871:930	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	21	theme	dibenzaldehyde-terminated	871:895	arg1	glycol					911:916	ethylene glycol	902:916	ethylene glycol	902:916	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	21	theme	dibenzaldehyde-terminated	871:895	arg1	DB-PEG2000					920:929	DB-PEG2000	920:929	DB-PEG2000	920:929	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	21	theme	dibenzaldehyde-terminated	871:895	arg1	components					803:812	only two components	794:812	only two components	794:812	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	11	22	theme	CQCS	1913:1916	arg1	gel					1918:1920	the CQCS@gel	1909:1920	the CQCS@gel with outstanding contact-active bacterial killing efficiency	1909:1981	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	13	23	theme	injectable	2247:2256	arg1	system					2267:2272	a single injectable hydrogel system	2238:2272	a single injectable hydrogel system with easy availability and low cost	2238:2308	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	9	24	theme	wound	1531:1535	arg1	dressing					1537:1544	a universal wound dressing	1519:1544	a universal wound dressing in clinical applications	1519:1569	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	9	24	theme	wound	1531:1535	arg1	potentials					1505:1514	potentials	1505:1514	potentials	1505:1514	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	14	25	theme	in	2324:2325	arg1	results					2332:2338	In vitro and in vivo results	2311:2338	In vitro and in vivo results	2311:2338	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	7	26	theme	hemostatic	1084:1093	arg1	study					1095:1099	the in vivo hemostatic study	1072:1099	the in vivo hemostatic study	1072:1099	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	2	27	theme	conventional	244:255	arg1	dressings					263:271	conventional wound dressings	244:271	conventional wound dressings (e.g., gauze, bandage)	244:294	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	2	27	theme	conventional	244:255	arg1	bandage					287:293	bandage	287:293	bandage	287:293	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	6	28	theme	@	965:965	arg1	gel					966:968	endowed CQCS@gel	953:968	endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	953:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	8	29	theme	recovery	1318:1325	arg1	process					1327:1333	the chronic recovery process	1306:1333	the chronic recovery process of MRSA-infected cutaneous wounds	1306:1367	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	7	30	theme	in	1076:1077	arg1	study					1095:1099	the in vivo hemostatic study	1072:1099	the in vivo hemostatic study	1072:1099	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	12	31	with	endowed	2108:2114	arg1	capabilities					2178:2189	self-healing and pH-responsive drug release capabilities	2134:2189	self-healing and pH-responsive drug release capabilities	2134:2189	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	11	32	theme	adhesive	2029:2036	arg1	properties					2054:2063	its tissue adhesive and antioxidant properties	2018:2063	its tissue adhesive and antioxidant properties	2018:2063	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	12	33	theme	pH-responsive	2151:2163	arg1	capabilities					2178:2189	self-healing and pH-responsive drug release capabilities	2134:2189	self-healing and pH-responsive drug release capabilities	2134:2189	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	14	34	theme	multiple	2444:2451	arg1	scenarios					2462:2470	multiple bleeding scenarios	2444:2470	multiple bleeding scenarios	2444:2470	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	7	35	theme	@	1262:1262	arg1	gel					1263:1265	CQCS@gel	1258:1265	CQCS@gel	1258:1265	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	14	36	dep	in	2324:2325	arg1	vivo					2327:2330	vivo	2327:2330	vivo	2327:2330	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	5	37	theme	bioinspired	722:732	arg1	gel					776:778	CQCS@gel	771:778	CQCS@gel	771:778	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	37	theme	bioinspired	722:732	arg1	hydrogel					761:768	a bioinspired multifunctional injectable hydrogel	720:768	a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000)	720:930	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	8	38	theme	cutaneous	1352:1360	arg1	wounds					1362:1367	MRSA-infected cutaneous wounds	1338:1367	MRSA-infected cutaneous wounds	1338:1367	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	14	39	theme	successful	2408:2417	arg1	hemostasis					2427:2436	successful sealing hemostasis	2408:2436	successful sealing hemostasis under multiple bleeding scenarios	2408:2470	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	5	40	theme	@	775:775	arg1	gel					776:778	CQCS@gel	771:778	CQCS@gel	771:778	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	40	theme	@	775:775	arg1	hydrogel					761:768	a bioinspired multifunctional injectable hydrogel	720:768	a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000)	720:930	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	10	41	theme	ethylene	1834:1841	arg1	poly					1829:1832	dibenzaldehyde-terminated poly	1803:1832	dibenzaldehyde-terminated poly(ethylene glycol)	1803:1849	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	41	theme	ethylene	1834:1841	arg1	glycol					1843:1848	ethylene glycol	1834:1848	ethylene glycol	1834:1848	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	9	42	theme	infected	1601:1608	arg1	management					1616:1625	infected wound management	1601:1625	infected wound management	1601:1625	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	0	43	theme	Infected	67:74	arg1	Management					82:91	Infected Wound Management	67:91	Infected Wound Management	67:91	Bioinspired Multifunctional Injectable Hydrogel for Hemostasis and Infected Wound Management.
36640951	3	44	theme	wound	491:495	arg1	dressings					497:505	multifunctional wound dressings	475:505	multifunctional wound dressings	475:505	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	3	44	theme	wound	491:495	arg1	hydrogels					436:444	injectable hydrogels	425:444	injectable hydrogels	425:444	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	8	45	theme	collagen	1382:1389	arg1	deposition					1391:1400	collagen deposition	1382:1400	collagen deposition	1382:1400	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	8	45	theme	collagen	1382:1389	arg1	follicles					1408:1416	hair follicles regeneration and angiogenesis	1403:1446	hair follicles regeneration and angiogenesis	1403:1446	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	1	46	theme	Routine	94:100	arg1	management					108:117	Routine wound management	94:117	Routine wound management	94:117	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	10	47	theme	multifunctional	1694:1708	arg1	hydrogel					1721:1728	the multifunctional injectable hydrogel	1690:1728	the multifunctional injectable hydrogel (CQCS@gel)	1690:1739	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	47	theme	multifunctional	1694:1708	arg1	gel					1736:1738	CQCS@gel	1731:1738	CQCS@gel	1731:1738	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	7	48	theme	rapid	1204:1208	arg1	rate					1219:1222	rapid gelation rate	1204:1222	rapid gelation rate	1204:1222	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	10	49	dep	STATEMENT	1628:1636	arg1	This					1657:1660	This	1657:1660	This	1657:1660	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	49	dep	STATEMENT	1628:1636	arg1	report					1675:1680	the first report	1665:1680	the first report showing the multifunctional injectable hydrogel (CQCS@gel)	1665:1739	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	11	50	with	gel	1918:1920	arg1	efficiency					1972:1981	outstanding contact-active bacterial killing efficiency	1927:1981	outstanding contact-active bacterial killing efficiency	1927:1981	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	14	51	theme	designed	2362:2369	arg1	biocompatible					2384:2396	biocompatible	2384:2396	biocompatible	2384:2396	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	14	51	theme	designed	2362:2369	arg1	hydrogel					2371:2378	the newly designed hydrogel	2352:2378	the newly designed hydrogel	2352:2378	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	3	52	theme	multifunctional	475:489	arg1	dressings					497:505	multifunctional wound dressings	475:505	multifunctional wound dressings	475:505	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	3	52	theme	multifunctional	475:489	arg1	hydrogels					436:444	injectable hydrogels	425:444	injectable hydrogels	425:444	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	0	53	theme	Injectable	28:37	arg1	Hydrogel					39:46	Multifunctional Injectable Hydrogel	12:46	Multifunctional Injectable Hydrogel for Hemostasis and Infected Wound Management	12:91	Bioinspired Multifunctional Injectable Hydrogel for Hemostasis and Infected Wound Management.
36640951	1	54	theme	significant	125:135	arg1	rebleeding					158:167	rebleeding	158:167	rebleeding	158:167	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	1	54	theme	significant	125:135	arg1	infection					183:191	bacterial infection	173:191	bacterial infection	173:191	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	1	54	theme	significant	125:135	arg1	challenges					137:146	significant challenges	125:146	significant challenges including rebleeding and bacterial infection that affect millions of people each year	125:232	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	10	55	theme	CQCS	1731:1734	arg1	hydrogel					1721:1728	the multifunctional injectable hydrogel	1690:1728	the multifunctional injectable hydrogel (CQCS@gel)	1690:1739	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	55	theme	CQCS	1731:1734	arg1	gel					1736:1738	CQCS@gel	1731:1738	CQCS@gel	1731:1738	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	8	56	dep	follicles	1408:1416	arg1	angiogenesis					1435:1446	angiogenesis	1435:1446	angiogenesis	1435:1446	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	8	56	dep	follicles	1408:1416	arg1	regeneration					1418:1429	regeneration	1418:1429	regeneration	1418:1429	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	8	56	dep	follicles	1408:1416	arg1	follicles					1408:1416	hair follicles regeneration and angiogenesis	1403:1446	hair follicles regeneration and angiogenesis	1403:1446	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	0	57	theme	Wound	76:80	arg1	Management					82:91	Infected Wound Management	67:91	Infected Wound Management	67:91	Bioinspired Multifunctional Injectable Hydrogel for Hemostasis and Infected Wound Management.
36640951	10	58	theme	catechol-functionalized	1754:1776	arg1	chitosan					1790:1797	catechol-functionalized quaternized chitosan	1754:1797	catechol-functionalized quaternized chitosan	1754:1797	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	5	59	theme	quaternized	839:849	arg1	components					803:812	only two components	794:812	only two components	794:812	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	59	theme	quaternized	839:849	arg1	CQCS					861:864	CQCS	861:864	CQCS	861:864	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	59	theme	quaternized	839:849	arg1	chitosan					851:858	catechol-functionalized quaternized chitosan	815:858	catechol-functionalized quaternized chitosan (CQCS)	815:865	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	13	60	with	system	2267:2272	arg1	availability					2284:2295	easy availability	2279:2295	easy availability	2279:2295	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	13	60	with	system	2267:2272	arg1	cost					2305:2308	low cost	2301:2308	low cost	2301:2308	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	11	61	theme	bacterial	1954:1962	arg1	killing					1964:1970	outstanding contact-active bacterial killing	1927:1970	outstanding contact-active bacterial killing efficiency	1927:1981	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	10	62	theme	dibenzaldehyde-terminated	1803:1827	arg1	poly					1829:1832	dibenzaldehyde-terminated poly	1803:1832	dibenzaldehyde-terminated poly(ethylene glycol)	1803:1849	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	62	theme	dibenzaldehyde-terminated	1803:1827	arg1	glycol					1843:1848	ethylene glycol	1834:1848	ethylene glycol	1834:1848	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	7	63	theme	carotid	1163:1169	arg1	wounds					1171:1176	carotid wounds	1163:1176	carotid wounds	1163:1176	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	6	64	theme	CQCS	961:964	arg1	gel					966:968	endowed CQCS@gel	953:968	endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	953:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	2	65	theme	special	361:367	arg1	functionality					369:381	special functionality to promote the wound recovery	361:411	special functionality to promote the wound recovery	361:411	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	4	66	theme	functions	601:609	arg1	integration					577:587	the integration	573:587	the integration of multiple functions through simple composition and easy construction	573:658	Nevertheless, the integration of multiple functions through simple composition and easy construction is still difficult and hardly achieved.
36640951	10	67	theme	SIGNIFICANCE	1641:1652	arg1	STATEMENT					1628:1636	STATEMENT	1628:1636	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel)	1628:1739	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	14	68	theme	wounds	2521:2526	arg1	healing					2496:2502	accelerated healing	2484:2502	accelerated healing of infected skin wounds	2484:2526	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	13	69	theme	easy	2279:2282	arg1	availability					2284:2295	easy availability	2279:2295	easy availability	2279:2295	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	12	70	theme	release	2170:2176	arg1	capabilities					2178:2189	self-healing and pH-responsive drug release capabilities	2134:2189	self-healing and pH-responsive drug release capabilities	2134:2189	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	4	71	theme	simple	619:624	arg1	composition					626:636	simple composition	619:636	simple composition	619:636	Nevertheless, the integration of multiple functions through simple composition and easy construction is still difficult and hardly achieved.
36640951	11	72	theme	outstanding	1927:1937	arg1	killing					1964:1970	outstanding contact-active bacterial killing	1927:1970	outstanding contact-active bacterial killing efficiency	1927:1981	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	7	73	theme	tissue	1129:1134	arg1	injuries					1136:1143	acute tissue injuries	1123:1143	acute tissue injuries such as liver and carotid wounds	1123:1176	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	7	73	theme	tissue	1129:1134	arg1	liver					1153:1157	liver	1153:1157	liver	1153:1157	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	7	73	theme	tissue	1129:1134	arg1	wounds					1171:1176	carotid wounds	1163:1176	carotid wounds	1163:1176	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	14	74	theme	infected	2507:2514	arg1	wounds					2521:2526	infected skin wounds	2507:2526	infected skin wounds	2507:2526	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	5	75	theme	ethylene	902:909	arg1	poly					897:900	dibenzaldehyde-terminated poly	871:900	dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000)	871:930	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	75	theme	ethylene	902:909	arg1	glycol					911:916	ethylene glycol	902:916	ethylene glycol	902:916	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	1	76	theme	people	217:222	arg1	year					229:232	people each year	217:232	people each year	217:232	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	6	77	theme	antioxidant	1007:1017	arg1	properties					1051:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	9	78	from	dressing	1537:1544	arg1	applications					1558:1569	clinical applications	1549:1569	clinical applications	1549:1569	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	2	79	dep	gauze	280:284	arg1	e.g.					274:277	e.g.	274:277	e.g.	274:277	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	11	80	theme	groups	1893:1898	arg1	incorporation					1856:1868	The incorporation	1852:1868	The incorporation of quaternary ammonium groups	1852:1898	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	5	81	theme	multifunctional	734:748	arg1	gel					776:778	CQCS@gel	771:778	CQCS@gel	771:778	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	81	theme	multifunctional	734:748	arg1	hydrogel					761:768	a bioinspired multifunctional injectable hydrogel	720:768	a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000)	720:930	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	1	82	theme	year	229:232	arg1	millions					205:212	millions	205:212	millions of people each year	205:232	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	12	83	theme	reversible	2080:2089	arg1	imine					2091:2095	the reversible imine	2076:2095	the reversible imine	2076:2095	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	6	84	theme	antibacterial	992:1004	arg1	properties					1051:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	12	85	theme	drug	2165:2168	arg1	capabilities					2178:2189	self-healing and pH-responsive drug release capabilities	2134:2189	self-healing and pH-responsive drug release capabilities	2134:2189	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	8	86	theme	CQCS	1278:1281	arg1	gel					1283:1285	CQCS@gel	1278:1285	CQCS@gel	1278:1285	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	11	87	theme	quaternary	1873:1882	arg1	ammonium					1884:1891	quaternary ammonium	1873:1891	quaternary ammonium groups	1873:1898	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	13	88	theme	single	2240:2245	arg1	system					2267:2272	a single injectable hydrogel system	2238:2272	a single injectable hydrogel system with easy availability and low cost	2238:2308	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	9	89	theme	universal	1521:1529	arg1	dressing					1537:1544	a universal wound dressing	1519:1544	a universal wound dressing in clinical applications	1519:1569	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	9	89	theme	universal	1521:1529	arg1	potentials					1505:1514	potentials	1505:1514	potentials	1505:1514	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	6	90	theme	tissue-adhesive	975:989	arg1	properties					1051:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	10	91	theme	first	1669:1673	arg1	This					1657:1660	This	1657:1660	This	1657:1660	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	91	theme	first	1669:1673	arg1	report					1675:1680	the first report	1665:1680	the first report showing the multifunctional injectable hydrogel (CQCS@gel)	1665:1739	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	11	92	theme	@	1917:1917	arg1	gel					1918:1920	the CQCS@gel	1909:1920	the CQCS@gel with outstanding contact-active bacterial killing efficiency	1909:1981	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	13	93	theme	hydrogel	2258:2265	arg1	system					2267:2272	a single injectable hydrogel system	2238:2272	a single injectable hydrogel system with easy availability and low cost	2238:2308	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	2	94	theme	wound	257:261	arg1	dressings					263:271	conventional wound dressings	244:271	conventional wound dressings (e.g., gauze, bandage)	244:294	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	2	94	theme	wound	257:261	arg1	bandage					287:293	bandage	287:293	bandage	287:293	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	7	95	theme	quick	1102:1106	arg1	hemostasis					1108:1117	quick hemostasis	1102:1117	quick hemostasis for acute tissue injuries such as liver and carotid wounds	1102:1176	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	9	96	theme	clinical	1549:1556	arg1	applications					1558:1569	clinical applications	1549:1569	clinical applications	1549:1569	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	8	97	theme	chronic	1310:1316	arg1	process					1327:1333	the chronic recovery process	1306:1333	the chronic recovery process of MRSA-infected cutaneous wounds	1306:1367	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	14	98	theme	bleeding	2453:2460	arg1	scenarios					2462:2470	multiple bleeding scenarios	2444:2470	multiple bleeding scenarios	2444:2470	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	7	99	theme	CQCS	1258:1261	arg1	gel					1263:1265	CQCS@gel	1258:1265	CQCS@gel	1258:1265	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	1	100	theme	bacterial	173:181	arg1	infection					183:191	bacterial infection	173:191	bacterial infection	173:191	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	14	101	dep	In	2311:2312	arg1	vitro					2314:2318	vitro	2314:2318	vitro	2314:2318	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	7	102	theme	strong	1228:1233	arg1	tissue-adhesiveness					1235:1253	strong tissue-adhesiveness	1228:1253	strong tissue-adhesiveness	1228:1253	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	6	103	theme	pH-responsive	1037:1049	arg1	properties					1051:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	8	104	theme	MRSA-infected	1338:1350	arg1	wounds					1362:1367	MRSA-infected cutaneous wounds	1338:1367	MRSA-infected cutaneous wounds	1338:1367	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	14	105	theme	sealing	2419:2425	arg1	hemostasis					2427:2436	successful sealing hemostasis	2408:2436	successful sealing hemostasis under multiple bleeding scenarios	2408:2470	In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.
36640951	5	106	theme	CQCS	771:774	arg1	gel					776:778	CQCS@gel	771:778	CQCS@gel	771:778	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	106	theme	CQCS	771:774	arg1	hydrogel					761:768	a bioinspired multifunctional injectable hydrogel	720:768	a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000)	720:930	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	13	107	theme	multiple	2198:2205	arg1	functions					2207:2215	These multiple functions	2192:2215	These multiple functions	2192:2215	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	6	108	theme	self-healing	1020:1031	arg1	properties					1051:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties	975:1060	The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties.
36640951	8	109	theme	wounds	1362:1367	arg1	process					1327:1333	the chronic recovery process	1306:1333	the chronic recovery process of MRSA-infected cutaneous wounds	1306:1367	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	7	110	theme	gelation	1210:1217	arg1	rate					1219:1222	rapid gelation rate	1204:1222	rapid gelation rate	1204:1222	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	9	111	theme	wound	1610:1614	arg1	management					1616:1625	infected wound management	1601:1625	infected wound management	1601:1625	Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.
36640951	2	112	theme	wound	398:402	arg1	recovery					404:411	the wound recovery	394:411	the wound recovery	394:411	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	0	113	theme	Multifunctional	12:26	arg1	Hydrogel					39:46	Multifunctional Injectable Hydrogel	12:46	Multifunctional Injectable Hydrogel for Hemostasis and Infected Wound Management	12:91	Bioinspired Multifunctional Injectable Hydrogel for Hemostasis and Infected Wound Management.
36640951	1	114	theme	wound	102:106	arg1	management					108:117	Routine wound management	94:117	Routine wound management	94:117	Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year.
36640951	11	115	theme	catechol	1991:1998	arg1	moieties					2000:2007	the catechol moieties	1987:2007	the catechol moieties	1987:2007	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	4	116	theme	multiple	592:599	arg1	functions					601:609	multiple functions	592:609	multiple functions	592:609	Nevertheless, the integration of multiple functions through simple composition and easy construction is still difficult and hardly achieved.
36640951	8	117	theme	hair	1403:1406	arg1	angiogenesis					1435:1446	angiogenesis	1435:1446	angiogenesis	1435:1446	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	8	117	theme	hair	1403:1406	arg1	regeneration					1418:1429	regeneration	1418:1429	regeneration	1418:1429	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	8	117	theme	hair	1403:1406	arg1	deposition					1391:1400	collagen deposition	1382:1400	collagen deposition	1382:1400	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	8	117	theme	hair	1403:1406	arg1	follicles					1408:1416	hair follicles regeneration and angiogenesis	1403:1446	hair follicles regeneration and angiogenesis	1403:1446	Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis.
36640951	10	118	theme	@	1735:1735	arg1	hydrogel					1721:1728	the multifunctional injectable hydrogel	1690:1728	the multifunctional injectable hydrogel (CQCS@gel)	1690:1739	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	118	theme	@	1735:1735	arg1	gel					1736:1738	CQCS@gel	1731:1738	CQCS@gel	1731:1738	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	3	119	theme	injectable	425:434	arg1	hydrogels					436:444	injectable hydrogels	425:444	injectable hydrogels	425:444	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	3	119	theme	injectable	425:434	arg1	dressings					497:505	multifunctional wound dressings	475:505	multifunctional wound dressings	475:505	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36640951	12	120	dep	crosslinks	2097:2106	arg1	endowed					2108:2114	endowed	2108:2114	endowed	2108:2114	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	5	121	theme	catechol-functionalized	815:837	arg1	components					803:812	only two components	794:812	only two components	794:812	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	121	theme	catechol-functionalized	815:837	arg1	CQCS					861:864	CQCS	861:864	CQCS	861:864	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	5	121	theme	catechol-functionalized	815:837	arg1	chitosan					851:858	catechol-functionalized quaternized chitosan	815:858	catechol-functionalized quaternized chitosan (CQCS)	815:865	Herein, we reported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene glycol) (DB-PEG2000).
36640951	12	122	theme	@	2124:2124	arg1	gel					2125:2127	the CQCS@gel	2116:2127	the CQCS@gel	2116:2127	Moreover, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities.
36640951	7	123	dep	rate	1219:1222	arg1	the					1200:1202	the	1200:1202	the	1200:1202	Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel.
36640951	11	124	theme	contact-active	1939:1952	arg1	killing					1964:1970	outstanding contact-active bacterial killing	1927:1970	outstanding contact-active bacterial killing efficiency	1927:1981	The incorporation of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties.
36640951	4	125	theme	easy	642:645	arg1	construction					647:658	easy construction	642:658	easy construction	642:658	Nevertheless, the integration of multiple functions through simple composition and easy construction is still difficult and hardly achieved.
36640951	2	126	theme	injured	328:334	arg1	surfaces					336:343	the injured surfaces	324:343	the injured surfaces	324:343	However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery.
36640951	13	127	theme	low	2301:2303	arg1	cost					2305:2308	low cost	2301:2308	low cost	2301:2308	These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost.
36640951	10	128	theme	injectable	1710:1719	arg1	hydrogel					1721:1728	the multifunctional injectable hydrogel	1690:1728	the multifunctional injectable hydrogel (CQCS@gel)	1690:1739	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	10	128	theme	injectable	1710:1719	arg1	gel					1736:1738	CQCS@gel	1731:1738	CQCS@gel	1731:1738	STATEMENT OF SIGNIFICANCE: : This is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol).
36640951	3	129	theme	healing	542:548	arg1	process					550:556	the hemostatic and wound healing process	517:556	the hemostatic and wound healing process	517:556	Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process.
36538078	0	0	theme	cell	91:94	arg1	formation					101:109	the collenchyma cell wall formation	75:109	the collenchyma cell wall formation	75:109	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	1	1	theme	individual	166:175	arg1	types					188:192	individual plant cell types	166:192	individual plant cell types	166:192	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	8	2	from	players	1366:1372	arg1	orchestra					1395:1403	the transcriptome orchestra	1377:1403	the transcriptome orchestra of collenchyma	1377:1418	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	1	3	theme	cell	261:264	arg1	formation					271:279	cell wall formation	261:279	cell wall formation	261:279	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	0	4	theme	collenchyma	79:89	arg1	formation					101:109	the collenchyma cell wall formation	75:109	the collenchyma cell wall formation	75:109	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	9	5	theme	modification	1709:1720	arg1	peculiarities					1656:1668	peculiarities	1656:1668	peculiarities of collenchyma cell wall formation and modification	1656:1720	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	1	6	theme	plant	177:181	arg1	types					188:192	individual plant cell types	166:192	individual plant cell types	166:192	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	1	7	theme	wall	266:269	arg1	formation					271:279	cell wall formation	261:279	cell wall formation	261:279	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	0	8	theme	formation	101:109	arg1	peculiarities					58:70	peculiarities	58:70	peculiarities of the collenchyma cell wall formation	58:109	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	8	9	theme	expression	1534:1543	arg1	patterns					1545:1552	expression patterns	1534:1552	expression patterns	1534:1552	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	4	10	theme	other	739:743	arg1	tissues					745:751	other tissues	739:751	other tissues due to its thickening	739:773	Thus, the processes associated with the formation of the primary cell wall in the collenchyma can be more pronounced compared to other tissues due to its thickening.
36538078	1	11	theme	formation	271:279	arg1	machinery					248:256	the molecular machinery	234:256	the molecular machinery of cell wall formation in specialized cells such as collenchyma	234:320	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	0	12	theme	wall	96:99	arg1	formation					101:109	the collenchyma cell wall formation	75:109	the collenchyma cell wall formation	75:109	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	10	13	theme	families	1876:1883	arg1	result					1837:1842	a result	1835:1842	a result	1835:1842	As a result, specific isoforms of multigene families were determined for further functional investigation.
36538078	10	13	theme	families	1876:1883	arg1	isoforms					1854:1861	specific isoforms	1845:1861	specific isoforms of multigene families	1845:1883	As a result, specific isoforms of multigene families were determined for further functional investigation.
36538078	4	14	theme	wall	680:683	arg1	formation					650:658	the formation	646:658	the formation of the primary cell wall in the collenchyma	646:702	Thus, the processes associated with the formation of the primary cell wall in the collenchyma can be more pronounced compared to other tissues due to its thickening.
36538078	7	15	theme	primary	1157:1163	arg1	formation					1175:1183	the primary cell wall formation	1153:1183	the primary cell wall formation during cell elongation	1153:1206	Genes encoding proteins involved in the primary cell wall formation during cell elongation, such as xyloglucan endotransglucosylase/hydrolases, expansins, and leucine-rich repeat proteins, were significantly activated in the collenchyma.
36538078	6	16	attach	isolated	1002:1009	arg2	strands					994:1000	collenchyma strands	982:1000	collenchyma strands isolated from young celery petioles	982:1036	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	6	16	attach	isolated	1002:1009	arg1	petioles					1029:1036	young celery petioles	1016:1036	young celery petioles	1016:1036	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	9	17	theme	substrates	1793:1802	arg1	abundance					1736:1744	the abundance	1732:1744	the abundance of upregulated transcripts and their potential substrates for revealed gene products	1732:1829	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	6	18	theme	celery	1022:1027	arg1	petioles					1029:1036	young celery petioles	1016:1036	young celery petioles	1016:1036	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	3	19	theme	cell	534:537	arg1	wall					539:542	the primary cell wall	522:542	the primary cell wall	522:542	The composition of the collenchyma cell wall resembles that of the primary cell wall and includes cellulose, xyloglucan, and pectin; lignin is absent.
36538078	4	20	from	formation	650:658	arg1	collenchyma					692:702	the collenchyma	688:702	the collenchyma	688:702	Thus, the processes associated with the formation of the primary cell wall in the collenchyma can be more pronounced compared to other tissues due to its thickening.
36538078	1	21	theme	cell	183:186	arg1	types					188:192	individual plant cell types	166:192	individual plant cell types	166:192	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	7	22	theme	cell	1165:1168	arg1	formation					1175:1183	the primary cell wall formation	1153:1183	the primary cell wall formation during cell elongation	1153:1206	Genes encoding proteins involved in the primary cell wall formation during cell elongation, such as xyloglucan endotransglucosylase/hydrolases, expansins, and leucine-rich repeat proteins, were significantly activated in the collenchyma.
36538078	5	23	theme	cell	784:787	arg1	walls					789:793	Primary cell walls	776:793	Primary cell walls intrinsic to different tissues	776:824	Primary cell walls intrinsic to different tissues may differ in structure and composition, which should be reflected at the transcriptomic level.
36538078	6	24	theme	other	1061:1065	arg1	parenchyma					1084:1093	parenchyma	1084:1093	parenchyma	1084:1093	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	6	24	theme	other	1061:1065	arg1	bundles					1108:1114	vascular bundles	1099:1114	vascular bundles	1099:1114	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	6	24	theme	other	1061:1065	arg1	tissues					1067:1073	other tissues	1061:1073	other tissues	1061:1073	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	2	25	theme	cell	405:408	arg1	wall					410:413	thickened cell wall	395:413	thickened cell wall	395:413	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	2	25	theme	cell	405:408	arg1	irregular					384:392	irregular	384:392	irregular	384:392	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	5	26	theme	Primary	776:782	arg1	walls					789:793	Primary cell walls	776:793	Primary cell walls intrinsic to different tissues	776:824	Primary cell walls intrinsic to different tissues may differ in structure and composition, which should be reflected at the transcriptomic level.
36538078	7	27	theme	cell	1192:1195	arg1	elongation					1197:1206	cell elongation	1192:1206	cell elongation	1192:1206	Genes encoding proteins involved in the primary cell wall formation during cell elongation, such as xyloglucan endotransglucosylase/hydrolases, expansins, and leucine-rich repeat proteins, were significantly activated in the collenchyma.
36538078	7	28	theme	leucine-rich	1276:1287	arg1	proteins					1296:1303	leucine-rich repeat proteins	1276:1303	leucine-rich repeat proteins	1276:1303	Genes encoding proteins involved in the primary cell wall formation during cell elongation, such as xyloglucan endotransglucosylase/hydrolases, expansins, and leucine-rich repeat proteins, were significantly activated in the collenchyma.
36538078	10	29	theme	specific	1845:1852	arg1	result					1837:1842	a result	1835:1842	a result	1835:1842	As a result, specific isoforms of multigene families were determined for further functional investigation.
36538078	10	29	theme	specific	1845:1852	arg1	isoforms					1854:1861	specific isoforms	1845:1861	specific isoforms of multigene families	1845:1883	As a result, specific isoforms of multigene families were determined for further functional investigation.
36538078	5	30	theme	transcriptomic	900:913	arg1	level					915:919	the transcriptomic level	896:919	the transcriptomic level	896:919	Primary cell walls intrinsic to different tissues may differ in structure and composition, which should be reflected at the transcriptomic level.
36538078	2	31	theme	thickened	395:403	arg1	wall					410:413	thickened cell wall	395:413	thickened cell wall	395:413	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	2	31	theme	thickened	395:403	arg1	irregular					384:392	irregular	384:392	irregular	384:392	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	0	32	theme	Transcriptome	0:12	arg1	profiling					14:22	Transcriptome profiling	0:22	Transcriptome profiling of celery petiole tissues	0:48	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	1	33	theme	specialized	284:294	arg1	cells					296:300	specialized cells	284:300	specialized cells such as collenchyma	284:320	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	1	33	theme	specialized	284:294	arg1	collenchyma					310:320	collenchyma	310:320	collenchyma	310:320	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	9	34	theme	cell	1685:1688	arg1	formation					1695:1703	collenchyma cell wall formation	1673:1703	collenchyma cell wall formation	1673:1703	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	2	35	theme	Plant	323:327	arg1	tissue					357:362	a mechanical tissue	344:362	a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation	344:456	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	2	35	theme	Plant	323:327	arg1	collenchyma					329:339	Plant collenchyma	323:339	Plant collenchyma	323:339	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	0	36	theme	celery	27:32	arg1	tissues					42:48	celery petiole tissues	27:48	celery petiole tissues	27:48	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	9	37	theme	wall	1690:1693	arg1	formation					1695:1703	collenchyma cell wall formation	1673:1703	collenchyma cell wall formation	1673:1703	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	9	38	theme	transcripts	1761:1771	arg1	abundance					1736:1744	the abundance	1732:1744	the abundance of upregulated transcripts and their potential substrates for revealed gene products	1732:1829	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	9	39	theme	biochemical	1614:1624	arg1	analyses					1626:1633	biochemical analyses	1614:1633	biochemical analyses	1614:1633	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	1	40	theme	potential	204:212	arg1	players					214:220	potential players	204:220	potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma	204:320	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	5	41	theme	intrinsic	795:803	arg1	walls					789:793	Primary cell walls	776:793	Primary cell walls intrinsic to different tissues	776:824	Primary cell walls intrinsic to different tissues may differ in structure and composition, which should be reflected at the transcriptomic level.
36538078	6	42	theme	first	930:934	arg1	time					936:939	the first time	926:939	the first time	926:939	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	4	43	theme	due	753:755	arg1	tissues					745:751	other tissues	739:751	other tissues due to its thickening	739:773	Thus, the processes associated with the formation of the primary cell wall in the collenchyma can be more pronounced compared to other tissues due to its thickening.
36538078	4	44	theme	cell	675:678	arg1	wall					680:683	the primary cell wall	663:683	the primary cell wall	663:683	Thus, the processes associated with the formation of the primary cell wall in the collenchyma can be more pronounced compared to other tissues due to its thickening.
36538078	8	45	theme	key	1362:1364	arg1	transcripts					1463:1473	xyloglucan endotransglucosylase/hydrolase transcripts	1421:1473	xyloglucan endotransglucosylase/hydrolase transcripts	1421:1473	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	8	45	theme	key	1362:1364	arg1	players					1366:1372	the key players	1358:1372	the key players in the transcriptome orchestra of collenchyma	1358:1418	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	5	46	theme	different	808:816	arg1	tissues					818:824	different tissues	808:824	different tissues	808:824	Primary cell walls intrinsic to different tissues may differ in structure and composition, which should be reflected at the transcriptomic level.
36538078	0	47	theme	tissues	42:48	arg1	profiling					14:22	Transcriptome profiling	0:22	Transcriptome profiling of celery petiole tissues	0:48	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	2	48	theme	mechanical	346:355	arg1	tissue					357:362	a mechanical tissue	344:362	a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation	344:456	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	2	48	theme	mechanical	346:355	arg1	collenchyma					329:339	Plant collenchyma	323:339	Plant collenchyma	323:339	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	9	49	theme	upregulated	1749:1759	arg1	transcripts					1761:1771	upregulated transcripts	1749:1771	upregulated transcripts	1749:1771	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	7	50	theme	xyloglucan	1217:1226	arg1	endotransglucosylase/hydrolases					1228:1258	xyloglucan endotransglucosylase/hydrolases	1217:1258	xyloglucan endotransglucosylase/hydrolases	1217:1258	Genes encoding proteins involved in the primary cell wall formation during cell elongation, such as xyloglucan endotransglucosylase/hydrolases, expansins, and leucine-rich repeat proteins, were significantly activated in the collenchyma.
36538078	8	51	theme	transcriptome	1381:1393	arg1	orchestra					1395:1403	the transcriptome orchestra	1377:1403	the transcriptome orchestra of collenchyma	1377:1418	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	6	52	theme	young	1016:1020	arg1	petioles					1029:1036	young celery petioles	1016:1036	young celery petioles	1016:1036	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	0	53	theme	petiole	34:40	arg1	tissues					42:48	celery petiole tissues	27:48	celery petiole tissues	27:48	Transcriptome profiling of celery petiole tissues reveals peculiarities of the collenchyma cell wall formation.
36538078	6	54	theme	transcriptome	955:967	arg1	profiling					969:977	transcriptome profiling	955:977	transcriptome profiling of collenchyma strands isolated from young celery petioles	955:1036	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	2	55	theme	cell	442:445	arg1	elongation					447:456	cell elongation	442:456	cell elongation	442:456	Plant collenchyma is a mechanical tissue characterized by an irregular, thickened cell wall and the ability to support cell elongation.
36538078	7	56	theme	repeat	1289:1294	arg1	proteins					1296:1303	leucine-rich repeat proteins	1276:1303	leucine-rich repeat proteins	1276:1303	Genes encoding proteins involved in the primary cell wall formation during cell elongation, such as xyloglucan endotransglucosylase/hydrolases, expansins, and leucine-rich repeat proteins, were significantly activated in the collenchyma.
36538078	7	57	theme	wall	1170:1173	arg1	formation					1175:1183	the primary cell wall formation	1153:1183	the primary cell wall formation during cell elongation	1153:1206	Genes encoding proteins involved in the primary cell wall formation during cell elongation, such as xyloglucan endotransglucosylase/hydrolases, expansins, and leucine-rich repeat proteins, were significantly activated in the collenchyma.
36538078	1	58	from	machinery	248:256	arg1	cells					296:300	specialized cells	284:300	specialized cells such as collenchyma	284:320	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	1	58	from	machinery	248:256	arg1	collenchyma					310:320	collenchyma	310:320	collenchyma	310:320	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	10	59	theme	multigene	1866:1874	arg1	families					1876:1883	multigene families	1866:1883	multigene families	1866:1883	As a result, specific isoforms of multigene families were determined for further functional investigation.
36538078	9	60	theme	revealed	1808:1815	arg1	products					1822:1829	revealed gene products	1808:1829	revealed gene products	1808:1829	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	3	61	theme	wall	499:502	arg1	composition					463:473	The composition	459:473	The composition of the collenchyma cell wall	459:502	The composition of the collenchyma cell wall resembles that of the primary cell wall and includes cellulose, xyloglucan, and pectin; lignin is absent.
36538078	9	62	theme	potential	1783:1791	arg1	substrates					1793:1802	their potential substrates	1777:1802	their potential substrates for revealed gene products	1777:1829	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	10	63	theme	functional	1913:1922	arg1	investigation					1924:1936	further functional investigation	1905:1936	further functional investigation	1905:1936	As a result, specific isoforms of multigene families were determined for further functional investigation.
36538078	1	64	theme	biochemical	130:140	arg1	analyses					142:149	biochemical analyses	130:149	biochemical analyses	130:149	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	9	65	theme	formation	1695:1703	arg1	peculiarities					1656:1668	peculiarities	1656:1668	peculiarities of collenchyma cell wall formation and modification	1656:1720	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	9	66	theme	comprehensive	1559:1571	arg1	approach					1573:1580	The comprehensive approach	1555:1580	The comprehensive approach that included transcriptome and biochemical analyses	1555:1633	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	8	67	theme	more	1497:1500	arg1	detail					1502:1507	more detail	1497:1507	more detail	1497:1507	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	9	68	theme	collenchyma	1673:1683	arg1	formation					1695:1703	collenchyma cell wall formation	1673:1703	collenchyma cell wall formation	1673:1703	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	6	69	theme	strands	994:1000	arg1	profiling					969:977	transcriptome profiling	955:977	transcriptome profiling of collenchyma strands isolated from young celery petioles	955:1036	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	3	70	theme	collenchyma	482:492	arg1	wall					499:502	the collenchyma cell wall	478:502	the collenchyma cell wall	478:502	The composition of the collenchyma cell wall resembles that of the primary cell wall and includes cellulose, xyloglucan, and pectin; lignin is absent.
36538078	9	71	theme	gene	1817:1820	arg1	products					1822:1829	revealed gene products	1808:1829	revealed gene products	1808:1829	The comprehensive approach that included transcriptome and biochemical analyses allowed us to reveal peculiarities of collenchyma cell wall formation and modification, matching the abundance of upregulated transcripts and their potential substrates for revealed gene products.
36538078	8	72	theme	collenchyma	1408:1418	arg1	orchestra					1395:1403	the transcriptome orchestra	1377:1403	the transcriptome orchestra of collenchyma	1377:1418	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	3	73	theme	cell	494:497	arg1	wall					499:502	the collenchyma cell wall	478:502	the collenchyma cell wall	478:502	The composition of the collenchyma cell wall resembles that of the primary cell wall and includes cellulose, xyloglucan, and pectin; lignin is absent.
36538078	6	74	theme	vascular	1099:1106	arg1	bundles					1108:1114	vascular bundles	1099:1114	vascular bundles	1099:1114	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	1	75	theme	molecular	238:246	arg1	machinery					248:256	the molecular machinery	234:256	the molecular machinery of cell wall formation in specialized cells such as collenchyma	234:320	Transcriptome and biochemical analyses are applied to individual plant cell types to reveal potential players involved in the molecular machinery of cell wall formation in specialized cells such as collenchyma.
36538078	8	76	theme	endotransglucosylase/hydrolase	1432:1461	arg1	transcripts					1463:1473	xyloglucan endotransglucosylase/hydrolase transcripts	1421:1473	xyloglucan endotransglucosylase/hydrolase transcripts	1421:1473	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	8	76	theme	endotransglucosylase/hydrolase	1432:1461	arg1	players					1366:1372	the key players	1358:1372	the key players in the transcriptome orchestra of collenchyma	1358:1418	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	4	77	theme	primary	667:673	arg1	wall					680:683	the primary cell wall	663:683	the primary cell wall	663:683	Thus, the processes associated with the formation of the primary cell wall in the collenchyma can be more pronounced compared to other tissues due to its thickening.
36538078	6	78	theme	collenchyma	982:992	arg1	strands					994:1000	collenchyma strands	982:1000	collenchyma strands isolated from young celery petioles	982:1036	For the first time, we conducted transcriptome profiling of collenchyma strands isolated from young celery petioles and compared them with other tissues, such as parenchyma and vascular bundles.
36538078	8	79	theme	xyloglucan	1421:1430	arg1	transcripts					1463:1473	xyloglucan endotransglucosylase/hydrolase transcripts	1421:1473	xyloglucan endotransglucosylase/hydrolase transcripts	1421:1473	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	8	79	theme	xyloglucan	1421:1430	arg1	players					1366:1372	the key players	1358:1372	the key players in the transcriptome orchestra of collenchyma	1358:1418	As the key players in the transcriptome orchestra of collenchyma, xyloglucan endotransglucosylase/hydrolase transcripts were characterized in more detail, including phylogeny and expression patterns.
36538078	3	80	theme	primary	526:532	arg1	wall					539:542	the primary cell wall	522:542	the primary cell wall	522:542	The composition of the collenchyma cell wall resembles that of the primary cell wall and includes cellulose, xyloglucan, and pectin; lignin is absent.
36538078	10	81	theme	further	1905:1911	arg1	investigation					1924:1936	further functional investigation	1905:1936	further functional investigation	1905:1936	As a result, specific isoforms of multigene families were determined for further functional investigation.
37299390	4	0	theme	High-throughput	494:508	arg1	technology					521:530	High-throughput sequencing technology	494:530	High-throughput sequencing technology	494:530	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	10	1	theme	impaired	1429:1436	arg1	absorption					1448:1457	impaired vitamin A absorption	1429:1457	impaired vitamin A absorption	1429:1457	DSS-induced colitis and impaired vitamin A absorption reduced ovarian function.
37299390	7	2	theme	metabolism-related	961:978	arg1	genes					980:984	vitamin A synthesis metabolism-related genes	941:984	vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ	941:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	2	theme	metabolism-related	961:978	arg1	Rdh10					986:990	Rdh10	986:990	Rdh10	986:990	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	2	theme	metabolism-related	961:978	arg1	Cyp26b1					1011:1017	Cyp26b1	1011:1017	Cyp26b1	1011:1017	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	2	theme	metabolism-related	961:978	arg1	Aldh1a1					993:999	Aldh1a1	993:999	Aldh1a1	993:999	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	2	theme	metabolism-related	961:978	arg1	Rarβ					1024:1027	Rarβ	1024:1027	Rarβ	1024:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	2	theme	metabolism-related	961:978	arg1	Cyp26a1					1002:1008	Cyp26a1	1002:1008	Cyp26a1	1002:1008	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	4	3	theme	flora	596:600	arg1	proportion					572:581	proportion	572:581	proportion	572:581	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	4	3	theme	flora	596:600	arg1	composition					556:566	composition	556:566	composition	556:566	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	4	3	theme	flora	596:600	arg1	changes					629:635	gene expression changes	613:635	the composition and proportion of bacterial flora as well as gene expression changes in the colon	552:648	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	6	4	theme	high	890:893	arg1	expression					895:904	high expression	890:904	high expression of inflammatory factors	890:928	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	10	5	theme	A	1446:1446	arg1	absorption					1448:1457	impaired vitamin A absorption	1429:1457	impaired vitamin A absorption	1429:1457	DSS-induced colitis and impaired vitamin A absorption reduced ovarian function.
37299390	7	6	theme	vitamin	941:947	arg1	genes					980:984	vitamin A synthesis metabolism-related genes	941:984	vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ	941:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	6	theme	vitamin	941:947	arg1	Rdh10					986:990	Rdh10	986:990	Rdh10	986:990	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	6	theme	vitamin	941:947	arg1	Cyp26b1					1011:1017	Cyp26b1	1011:1017	Cyp26b1	1011:1017	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	6	theme	vitamin	941:947	arg1	Aldh1a1					993:999	Aldh1a1	993:999	Aldh1a1	993:999	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	6	theme	vitamin	941:947	arg1	Rarβ					1024:1027	Rarβ	1024:1027	Rarβ	1024:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	6	theme	vitamin	941:947	arg1	Cyp26a1					1002:1008	Cyp26a1	1002:1008	Cyp26a1	1002:1008	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	6	7	theme	cell	839:842	arg1	infiltration					844:855	increased inflammatory cell infiltration	816:855	increased inflammatory cell infiltration	816:855	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	1	8	theme	nutrient	246:253	arg1	absorption					255:264	nutrient absorption	246:264	nutrient absorption	246:264	Changes in the composition and ratio of the flora during colitis have been found to potentially affect ovarian function through nutrient absorption.
37299390	5	9	theme	follicle	662:669	arg1	type					671:674	follicle type	662:674	follicle type	662:674	Changes in follicle type, number, and hormone secretion in the ovary were detected.
37299390	6	10	theme	colitis	788:794	arg1	infiltration					844:855	increased inflammatory cell infiltration	816:855	increased inflammatory cell infiltration	816:855	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	10	theme	colitis	788:794	arg1	damage					865:870	severe damage	858:870	severe damage to the crypt	858:883	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	10	theme	colitis	788:794	arg1	expression					895:904	high expression	890:904	high expression of inflammatory factors	890:928	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	10	theme	colitis	788:794	arg1	symptoms					796:803	severe colitis symptoms	781:803	severe colitis symptoms	781:803	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	0	11	theme	Ovarian	92:98	arg1	Function					100:107	Ovarian Function	92:107	Ovarian Function in Mice	92:115	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	10	12	theme	DSS-induced	1405:1415	arg1	colitis					1417:1423	DSS-induced colitis	1405:1423	DSS-induced colitis	1405:1423	DSS-induced colitis and impaired vitamin A absorption reduced ovarian function.
37299390	6	13	theme	severe	781:786	arg1	infiltration					844:855	increased inflammatory cell infiltration	816:855	increased inflammatory cell infiltration	816:855	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	13	theme	severe	781:786	arg1	damage					865:870	severe damage	858:870	severe damage to the crypt	858:883	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	13	theme	severe	781:786	arg1	expression					895:904	high expression	890:904	high expression of inflammatory factors	890:928	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	13	theme	severe	781:786	arg1	symptoms					796:803	severe colitis symptoms	781:803	severe colitis symptoms	781:803	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	8	14	theme	Anti-Mullerian	1191:1204	arg1	hormone					1206:1212	Anti-Mullerian hormone	1191:1212	Anti-Mullerian hormone	1191:1212	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	9	15	theme	Bacteroides	1338:1348	arg1	abundances					1299:1308	The significantly changed abundances	1273:1308	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora	1273:1370	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	3	16	theme	dextran	441:447	arg1	DSS					465:467	DSS	465:467	DSS	465:467	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	3	16	theme	dextran	441:447	arg1	sulfate					456:462	dextran sodium sulfate	441:462	dextran sodium sulfate (DSS)	441:468	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	3	17	theme	colitis-induced	344:358	arg1	dysbacteriosis					360:373	colitis-induced dysbacteriosis	344:373	colitis-induced dysbacteriosis of the intestinal flora	344:397	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	9	18	theme	important	1388:1396	arg1	roles					1398:1402	potentially important roles	1376:1402	potentially important roles	1376:1402	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	7	19	theme	synthase-related	1104:1119	arg1	proteins					1121:1128	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	19	theme	synthase-related	1104:1119	arg1	CYP11A1					1139:1145	CYP11A1	1139:1145	CYP11A1	1139:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	19	theme	synthase-related	1104:1119	arg1	STAR					1130:1133	STAR	1130:1133	STAR	1130:1133	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	10	20	theme	ovarian	1467:1473	arg1	function					1475:1482	ovarian function	1467:1482	ovarian function	1467:1482	DSS-induced colitis and impaired vitamin A absorption reduced ovarian function.
37299390	4	21	from	proportion	572:581	arg1	colon					644:648	the colon	640:648	the colon	640:648	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	1	22	theme	flora	162:166	arg1	ratio					149:153	ratio	149:153	ratio	149:153	Changes in the composition and ratio of the flora during colitis have been found to potentially affect ovarian function through nutrient absorption.
37299390	1	22	theme	flora	162:166	arg1	composition					133:143	composition	133:143	composition	133:143	Changes in the composition and ratio of the flora during colitis have been found to potentially affect ovarian function through nutrient absorption.
37299390	3	23	theme	flora	393:397	arg1	dysbacteriosis					360:373	colitis-induced dysbacteriosis	344:373	colitis-induced dysbacteriosis of the intestinal flora	344:397	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	4	24	used	used	536:539	arg2	technology					521:530	High-throughput sequencing technology	494:530	High-throughput sequencing technology	494:530	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	4	25	from	changes	629:635	arg1	colon					644:648	the colon	640:648	the colon	640:648	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	0	26	theme	Colitis-Mediated	0:15	arg1	Dysbiosis					17:25	Colitis-Mediated Dysbiosis	0:25	Colitis-Mediated Dysbiosis of the Intestinal Flora	0:49	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	7	27	theme	proteins	1121:1128	arg1	levels					1074:1079	the levels	1070:1079	the levels of the steroid hormone synthase-related proteins STAR and CYP11A1	1070:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	28	dep	proteins	1121:1128	arg1	proteins					1121:1128	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	28	dep	proteins	1121:1128	arg1	CYP11A1					1139:1145	CYP11A1	1139:1145	CYP11A1	1139:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	28	dep	proteins	1121:1128	arg1	STAR					1130:1133	STAR	1130:1133	STAR	1130:1133	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	6	29	theme	%	762:762	arg1	DSS					764:766	2.5% DSS	759:766	2.5% DSS	759:766	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	8	30	theme	estradiol	1162:1170	arg1	quality					1229:1235	the quality	1225:1235	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	8	30	theme	estradiol	1162:1170	arg1	levels					1152:1157	The levels	1148:1157	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	9	31	theme	Alistipes	1313:1321	arg1	abundances					1299:1308	The significantly changed abundances	1273:1308	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora	1273:1370	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	5	32	theme	hormone	689:695	arg1	secretion					697:705	hormone secretion	689:705	hormone secretion	689:705	Changes in follicle type, number, and hormone secretion in the ovary were detected.
37299390	1	33	from	Changes	118:124	arg1	ratio					149:153	ratio	149:153	ratio	149:153	Changes in the composition and ratio of the flora during colitis have been found to potentially affect ovarian function through nutrient absorption.
37299390	1	33	from	Changes	118:124	arg1	composition					133:143	composition	133:143	composition	133:143	Changes in the composition and ratio of the flora during colitis have been found to potentially affect ovarian function through nutrient absorption.
37299390	1	34	dep	composition	133:143	arg1	the					129:131	the	129:131	the	129:131	Changes in the composition and ratio of the flora during colitis have been found to potentially affect ovarian function through nutrient absorption.
37299390	9	35	contain	had	1372:1374	arg2	roles					1398:1402	potentially important roles	1376:1402	potentially important roles	1376:1402	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	9	35	contain	had	1372:1374	arg1	abundances					1299:1308	The significantly changed abundances	1273:1308	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora	1273:1370	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	3	36	theme	intestinal	382:391	arg1	flora					393:397	the intestinal flora	378:397	the intestinal flora	378:397	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	6	37	theme	inflammatory	909:920	arg1	factors					922:928	inflammatory factors	909:928	inflammatory factors	909:928	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	38	theme	severe	858:863	arg1	damage					865:870	severe damage	858:870	severe damage to the crypt	858:883	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	7	39	dep	genes	980:984	arg1	genes					980:984	vitamin A synthesis metabolism-related genes	941:984	vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ	941:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	39	dep	genes	980:984	arg1	Rdh10					986:990	Rdh10	986:990	Rdh10	986:990	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	39	dep	genes	980:984	arg1	Cyp26b1					1011:1017	Cyp26b1	1011:1017	Cyp26b1	1011:1017	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	39	dep	genes	980:984	arg1	Aldh1a1					993:999	Aldh1a1	993:999	Aldh1a1	993:999	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	39	dep	genes	980:984	arg1	Rarβ					1024:1027	Rarβ	1024:1027	Rarβ	1024:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	39	dep	genes	980:984	arg1	Cyp26a1					1002:1008	Cyp26a1	1002:1008	Cyp26a1	1002:1008	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	0	40	theme	Flora	45:49	arg1	Absorption					74:83	Impaired Vitamin A Absorption	55:83	Impaired Vitamin A Absorption	55:83	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	0	40	theme	Flora	45:49	arg1	Dysbiosis					17:25	Colitis-Mediated Dysbiosis	0:25	Colitis-Mediated Dysbiosis of the Intestinal Flora	0:49	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	7	41	theme	steroid	1088:1094	arg1	proteins					1121:1128	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	41	theme	steroid	1088:1094	arg1	CYP11A1					1139:1145	CYP11A1	1139:1145	CYP11A1	1139:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	41	theme	steroid	1088:1094	arg1	STAR					1130:1133	STAR	1130:1133	STAR	1130:1133	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	0	42	theme	Intestinal	34:43	arg1	Flora					45:49	the Intestinal Flora	30:49	the Intestinal Flora	30:49	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	4	43	theme	bacterial	586:594	arg1	flora					596:600	bacterial flora	586:600	bacterial flora	586:600	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	9	44	theme	flora	1366:1370	arg1	abundances					1299:1308	The significantly changed abundances	1273:1308	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora	1273:1370	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	3	45	theme	ovarian	407:413	arg1	function					415:422	ovarian function	407:422	ovarian function	407:422	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	6	46	theme	factors	922:928	arg1	infiltration					844:855	increased inflammatory cell infiltration	816:855	increased inflammatory cell infiltration	816:855	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	46	theme	factors	922:928	arg1	damage					865:870	severe damage	858:870	severe damage to the crypt	858:883	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	46	theme	factors	922:928	arg1	expression					895:904	high expression	890:904	high expression of inflammatory factors	890:928	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	8	47	theme	progesterone	1173:1184	arg1	quality					1229:1235	the quality	1225:1235	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	8	47	theme	progesterone	1173:1184	arg1	levels					1152:1157	The levels	1148:1157	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	0	48	theme	Vitamin	64:70	arg1	Absorption					74:83	Impaired Vitamin A Absorption	55:83	Impaired Vitamin A Absorption	55:83	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	9	49	theme	changed	1291:1297	arg1	abundances					1299:1308	The significantly changed abundances	1273:1308	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora	1273:1370	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	7	50	theme	hormone	1096:1102	arg1	proteins					1121:1128	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	the steroid hormone synthase-related proteins STAR and CYP11A1	1084:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	50	theme	hormone	1096:1102	arg1	CYP11A1					1139:1145	CYP11A1	1139:1145	CYP11A1	1139:1145	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	50	theme	hormone	1096:1102	arg1	STAR					1130:1133	STAR	1130:1133	STAR	1130:1133	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	5	51	from	Changes	651:657	arg1	secretion					697:705	hormone secretion	689:705	hormone secretion	689:705	Changes in follicle type, number, and hormone secretion in the ovary were detected.
37299390	5	51	from	Changes	651:657	arg1	ovary					714:718	the ovary	710:718	the ovary	710:718	Changes in follicle type, number, and hormone secretion in the ovary were detected.
37299390	5	51	from	Changes	651:657	arg1	number					677:682	number	677:682	number	677:682	Changes in follicle type, number, and hormone secretion in the ovary were detected.
37299390	5	51	from	Changes	651:657	arg1	type					671:674	follicle type	662:674	follicle type	662:674	Changes in follicle type, number, and hormone secretion in the ovary were detected.
37299390	6	52	theme	inflammatory	826:837	arg1	infiltration					844:855	increased inflammatory cell infiltration	816:855	increased inflammatory cell infiltration	816:855	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	8	53	theme	oocytes	1240:1246	arg1	quality					1229:1235	the quality	1225:1235	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	8	53	theme	oocytes	1240:1246	arg1	levels					1152:1157	The levels	1148:1157	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	6	54	theme	2.5	759:761	arg1	%					762:762	%	762:762	%	762:762	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	6	55	theme	increased	816:824	arg1	infiltration					844:855	increased inflammatory cell infiltration	816:855	increased inflammatory cell infiltration	816:855	The results showed that 2.5% DSS could induce severe colitis symptoms, including increased inflammatory cell infiltration, severe damage to the crypt, and high expression of inflammatory factors.
37299390	7	56	theme	A	949:949	arg1	genes					980:984	vitamin A synthesis metabolism-related genes	941:984	vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ	941:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	56	theme	A	949:949	arg1	Rdh10					986:990	Rdh10	986:990	Rdh10	986:990	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	56	theme	A	949:949	arg1	Cyp26b1					1011:1017	Cyp26b1	1011:1017	Cyp26b1	1011:1017	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	56	theme	A	949:949	arg1	Aldh1a1					993:999	Aldh1a1	993:999	Aldh1a1	993:999	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	56	theme	A	949:949	arg1	Rarβ					1024:1027	Rarβ	1024:1027	Rarβ	1024:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	56	theme	A	949:949	arg1	Cyp26a1					1002:1008	Cyp26a1	1002:1008	Cyp26a1	1002:1008	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	4	57	theme	gene	613:616	arg1	changes					629:635	gene expression changes	613:635	the composition and proportion of bacterial flora as well as gene expression changes in the colon	552:648	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	0	58	from	Function	100:107	arg1	Mice					112:115	Mice	112:115	Mice	112:115	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	9	59	theme	other	1360:1364	arg1	flora					1366:1370	some other flora	1355:1370	some other flora	1355:1370	The significantly changed abundances of Alistipes, Helicobacter, Bacteroides, and some other flora had potentially important roles.
37299390	0	60	theme	A	72:72	arg1	Absorption					74:83	Impaired Vitamin A Absorption	55:83	Impaired Vitamin A Absorption	55:83	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	10	61	theme	vitamin	1438:1444	arg1	absorption					1448:1457	impaired vitamin A absorption	1429:1457	impaired vitamin A absorption	1429:1457	DSS-induced colitis and impaired vitamin A absorption reduced ovarian function.
37299390	3	62	theme	sodium	449:454	arg1	DSS					465:467	DSS	465:467	DSS	465:467	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	3	62	theme	sodium	449:454	arg1	sulfate					456:462	dextran sodium sulfate	441:462	dextran sodium sulfate (DSS)	441:468	To investigate whether colitis-induced dysbacteriosis of the intestinal flora affects ovarian function, mice were given dextran sodium sulfate (DSS) through drinking water.
37299390	1	63	theme	ovarian	221:227	arg1	function					229:236	ovarian function	221:236	ovarian function	221:236	Changes in the composition and ratio of the flora during colitis have been found to potentially affect ovarian function through nutrient absorption.
37299390	0	64	theme	Impaired	55:62	arg1	Absorption					74:83	Impaired Vitamin A Absorption	55:83	Impaired Vitamin A Absorption	55:83	Colitis-Mediated Dysbiosis of the Intestinal Flora and Impaired Vitamin A Absorption Reduce Ovarian Function in Mice.
37299390	4	65	theme	sequencing	510:519	arg1	technology					521:530	High-throughput sequencing technology	494:530	High-throughput sequencing technology	494:530	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	4	66	from	composition	556:566	arg1	colon					644:648	the colon	640:648	the colon	640:648	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37299390	7	67	theme	synthesis	951:959	arg1	genes					980:984	vitamin A synthesis metabolism-related genes	941:984	vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ	941:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	67	theme	synthesis	951:959	arg1	Rdh10					986:990	Rdh10	986:990	Rdh10	986:990	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	67	theme	synthesis	951:959	arg1	Cyp26b1					1011:1017	Cyp26b1	1011:1017	Cyp26b1	1011:1017	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	67	theme	synthesis	951:959	arg1	Aldh1a1					993:999	Aldh1a1	993:999	Aldh1a1	993:999	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	67	theme	synthesis	951:959	arg1	Rarβ					1024:1027	Rarβ	1024:1027	Rarβ	1024:1027	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	7	67	theme	synthesis	951:959	arg1	Cyp26a1					1002:1008	Cyp26a1	1002:1008	Cyp26a1	1002:1008	Moreover, vitamin A synthesis metabolism-related genes Rdh10, Aldh1a1, Cyp26a1, Cyp26b1, and Rarβ were significantly decreased, as well as the levels of the steroid hormone synthase-related proteins STAR and CYP11A1.
37299390	8	68	theme	hormone	1206:1212	arg1	quality					1229:1235	the quality	1225:1235	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	8	68	theme	hormone	1206:1212	arg1	levels					1152:1157	The levels	1148:1157	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes	1148:1246	The levels of estradiol, progesterone, and Anti-Mullerian hormone as well as the quality of oocytes decreased significantly.
37299390	4	69	theme	expression	618:627	arg1	changes					629:635	gene expression changes	613:635	the composition and proportion of bacterial flora as well as gene expression changes in the colon	552:648	High-throughput sequencing technology was used to clarify the composition and proportion of bacterial flora as well as gene expression changes in the colon.
37175691	7	0	theme	INS-1E	1293:1298	arg1	cells					1300:1304	INS-1E cells	1293:1304	INS-1E cells	1293:1304	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	10	1	theme	insulin	2105:2111	arg1	secretion					2113:2121	insulin secretion	2105:2121	insulin secretion	2105:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	0	2	theme	High-Fat	157:164	arg1	Diet					166:169	a High-Fat Diet	155:169	a High-Fat Diet	155:169	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	8	3	theme	high-fat	1673:1680	arg1	diet					1682:1685	a high-fat diet	1671:1685	a high-fat diet	1671:1685	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	7	4	theme	farnesiana	1217:1226	arg1	extract					1241:1247	Vachellia farnesiana polyphenolic extract	1207:1247	Vachellia farnesiana polyphenolic extract	1207:1247	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	4	theme	farnesiana	1217:1226	arg1	VFPE					1201:1204	a VFPE	1199:1204	a VFPE (Vachellia farnesiana polyphenolic extract)	1199:1248	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	8	5	theme	energy	1445:1450	arg1	expenditure					1452:1462	whole-body energy expenditure	1434:1462	whole-body energy expenditure	1434:1462	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	3	6	with	activities	511:520	arg1	effects					550:556	potential anti-obesity effects	527:556	potential anti-obesity effects	527:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	8	7	theme	insulin	1521:1527	arg1	resistance					1529:1538	insulin resistance	1521:1538	insulin resistance	1521:1538	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	7	8	theme	glucose-stimulated	1253:1270	arg1	secretion					1280:1288	glucose-stimulated insulin secretion	1253:1288	glucose-stimulated insulin secretion in INS-1E cells	1253:1304	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	9	theme	INS-1E	1724:1729	arg1	cells					1731:1735	INS-1E cells	1724:1735	INS-1E cells	1724:1735	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	3	10	theme	potential	527:535	arg1	effects					550:556	potential anti-obesity effects	527:556	potential anti-obesity effects	527:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	4	11	from	experiments	655:665	arg1	mice					637:640	mice	637:640	mice	637:640	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	5	12	theme	%	824:824	arg1	gallate					833:839	0.1% methyl gallate	821:839	0.1% methyl gallate	821:839	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	6	13	theme	mice	1010:1013	arg1	mice					1010:1013	mice	1010:1013	mice	1010:1013	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	6	13	theme	mice	1010:1013	arg1	groups					1000:1005	two groups	996:1005	two groups of mice	996:1013	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	10	14	theme	insulin	1932:1938	arg1	resistance					1940:1949	insulin resistance	1932:1949	insulin resistance	1932:1949	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	4	15	theme	different	672:680	arg1	types					687:691	different cell types	672:691	different cell types	672:691	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	6	16	theme	10	1104:1105	arg1	%					1106:1106	%	1106:1106	%	1106:1106	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	0	17	attach	Derived	52:58	arg1	Them					65:68	Them	65:68	Them	65:68	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	0	17	attach	Derived	52:58	arg2	Pods					21:24	Vachellia farnesiana Pods	0:24	Vachellia farnesiana Pods	0:24	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	10	18	theme	renal	1968:1972	arg1	damage					1974:1979	hepatic and renal damage	1956:1979	hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion	1956:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	5	19	theme	preventive	701:710	arg1	study					712:716	the preventive study	697:716	the preventive study	697:716	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	20	theme	farnesiana	938:947	arg1	pods					949:952	Vachellia farnesiana pods	928:952	Vachellia farnesiana pods	928:952	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	20	theme	farnesiana	938:947	arg1	VFP					923:925	VFP	923:925	VFP (Vachellia farnesiana pods)	923:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	2	21	theme	Alternative	273:283	arg1	sources					295:301	Alternative vegetable sources	273:301	Alternative vegetable sources rich in polyphenols	273:321	Alternative vegetable sources rich in polyphenols are known to prevent or delay the progression of metabolic abnormalities during obesity.
37175691	1	22	theme	systemic	187:194	arg1	inflammation					196:207	systemic inflammation	187:207	systemic inflammation	187:207	Obesity causes systemic inflammation, hepatic and renal damage, as well as gut microbiota dysbiosis.
37175691	10	23	theme	systemic	1909:1916	arg1	inflammation					1918:1929	systemic inflammation	1909:1929	systemic inflammation	1909:1929	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	10	24	theme	hepatic	1956:1962	arg1	damage					1974:1979	hepatic and renal damage	1956:1979	hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion	1956:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	4	25	theme	in	646:647	arg1	experiments					655:665	in vitro experiments	646:665	in vitro experiments with different cell types	646:691	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	6	26	theme	additional	1129:1138	arg1	weeks					1140:1144	ten additional weeks	1125:1144	ten additional weeks	1125:1144	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	8	27	theme	exerted	1579:1585	arg1	effects					1604:1610	exerted immunomodulatory effects	1579:1610	exerted immunomodulatory effects	1579:1610	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	7	28	from	effect	1189:1194	arg1	secretion					1280:1288	glucose-stimulated insulin secretion	1253:1288	glucose-stimulated insulin secretion in INS-1E cells	1253:1304	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	6	29	theme	high-fat	1044:1051	arg1	diet					1053:1056	a high-fat diet	1042:1056	a high-fat diet	1042:1056	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	6	30	theme	powdered	1108:1115	arg1	VFP					1117:1119	10% powdered VFP	1104:1119	10% powdered VFP	1104:1119	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	4	31	with	preventive	583:592	arg1	types					687:691	different cell types	672:691	different cell types	672:691	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	9	32	theme	mitochondrial	1805:1817	arg1	activity					1819:1826	mitochondrial activity	1805:1826	mitochondrial activity in primary hepatocytes and C2C12 myotubes	1805:1868	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	10	33	theme	high-fat	1995:2002	arg1	diet					2004:2007	a high-fat diet	1993:2007	a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion	1993:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	5	34	theme	Control	753:759	arg1	diet					761:764	a Control diet	751:764	a Control diet	751:764	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	8	35	theme	fecal	1626:1630	arg1	composition					1647:1657	reshaped fecal gut microbiota composition	1617:1657	reshaped fecal gut microbiota composition	1617:1657	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	5	36	theme	powdered	846:853	arg1	VFP					855:857	10% powdered VFP	842:857	10% powdered VFP	842:857	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	2	37	from	rich	303:306	arg1	polyphenols					311:321	polyphenols	311:321	polyphenols	311:321	Alternative vegetable sources rich in polyphenols are known to prevent or delay the progression of metabolic abnormalities during obesity.
37175691	7	38	theme	primary	1370:1376	arg1	hepatocytes					1378:1388	primary hepatocytes	1370:1388	primary hepatocytes	1370:1388	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	4	39	from	preventive	583:592	arg1	mice					637:640	mice	637:640	mice	637:640	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	8	40	theme	microbiota	1636:1645	arg1	composition					1647:1657	reshaped fecal gut microbiota composition	1617:1657	reshaped fecal gut microbiota composition	1617:1657	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	9	41	theme	C2C12	1855:1859	arg1	myotubes					1861:1868	C2C12 myotubes	1855:1868	C2C12 myotubes	1855:1868	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	2	42	theme	metabolic	372:380	arg1	abnormalities					382:394	metabolic abnormalities	372:394	metabolic abnormalities	372:394	Alternative vegetable sources rich in polyphenols are known to prevent or delay the progression of metabolic abnormalities during obesity.
37175691	5	43	contain	containing	803:812	arg1	diet					798:801	a high-fat diet	787:801	a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	787:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	43	contain	containing	803:812	arg2	gallate					833:839	0.1% methyl gallate	821:839	0.1% methyl gallate	821:839	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	43	contain	containing	803:812	arg2	%					866:866	0.5%	863:866	0.5%	863:866	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	43	contain	containing	803:812	arg2	%					870:870	1%	869:870	1%	869:870	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	43	contain	containing	803:812	arg2	VFP					855:857	10% powdered VFP	842:857	10% powdered VFP	842:857	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	43	contain	containing	803:812	arg2	extract					897:903	a polyphenolic extract	882:903	a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	882:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	43	contain	containing	803:812	arg2	%					877:877	2%	876:877	2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	876:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	43	contain	containing	803:812	arg2	PE					906:907	PE	906:907	PE	906:907	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	3	44	theme	Vachellia	412:420	arg1	source					450:455	a potent source	441:455	a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects	441:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	3	44	theme	Vachellia	412:420	arg1	VF					434:435	VF	434:435	VF	434:435	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	3	44	theme	Vachellia	412:420	arg1	farnesiana					422:431	Vachellia farnesiana	412:431	Vachellia farnesiana (VF)	412:436	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	0	45	theme	Vachellia	0:8	arg1	Pods					21:24	Vachellia farnesiana Pods	0:24	Vachellia farnesiana Pods	0:24	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	1	46	theme	gut	247:249	arg1	dysbiosis					262:270	gut microbiota dysbiosis	247:270	gut microbiota dysbiosis	247:270	Obesity causes systemic inflammation, hepatic and renal damage, as well as gut microbiota dysbiosis.
37175691	10	47	theme	improved	2055:2062	arg1	function					2078:2085	improved mitochondrial function	2055:2085	improved mitochondrial function	2055:2085	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	9	48	dep	compounds	1755:1763	arg1	naringenin					1765:1774	naringenin	1765:1774	naringenin	1765:1774	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	48	dep	compounds	1755:1763	arg1	gallate					1787:1793	methyl gallate	1780:1793	methyl gallate	1780:1793	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	48	dep	compounds	1755:1763	arg1	compounds					1755:1763	its isolated compounds	1742:1763	its isolated compounds naringenin and methyl gallate	1742:1793	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	49	from	activity	1358:1365	arg1	hepatocytes					1378:1388	primary hepatocytes	1370:1388	primary hepatocytes	1370:1388	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	49	from	activity	1358:1365	arg1	myotubes					1400:1407	C2C12 myotubes	1394:1407	C2C12 myotubes	1394:1407	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	50	theme	gallate	1333:1339	arg1	effect					1189:1194	the effect	1185:1194	the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes	1185:1407	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	10	51	from	resistance	1940:1949	arg1	mice					1984:1987	mice	1984:1987	mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion	1984:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	3	52	theme	polyphenols	460:470	arg1	source					450:455	a potent source	441:455	a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects	441:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	3	52	theme	polyphenols	460:470	arg1	farnesiana					422:431	Vachellia farnesiana	412:431	Vachellia farnesiana (VF)	412:436	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	5	53	theme	high-fat	769:776	arg1	diet					778:781	a high-fat diet	767:781	a high-fat diet	767:781	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	6	54	with	diet	1094:1097	arg1	VFP					1117:1119	10% powdered VFP	1104:1119	10% powdered VFP	1104:1119	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	10	55	from	damage	1974:1979	arg1	mice					1984:1987	mice	1984:1987	mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion	1984:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	3	56	theme	anti-inflammatory	493:509	arg1	activities					511:520	antioxidant and anti-inflammatory activities	477:520	antioxidant and anti-inflammatory activities with potential anti-obesity effects	477:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	8	57	theme	mitochondrial	1468:1480	arg1	activity					1482:1489	mitochondrial activity	1468:1489	mitochondrial activity	1468:1489	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	1	58	theme	renal	222:226	arg1	damage					228:233	renal damage	222:233	renal damage	222:233	Obesity causes systemic inflammation, hepatic and renal damage, as well as gut microbiota dysbiosis.
37175691	5	59	theme	extract	897:903	arg1	extract					897:903	a polyphenolic extract	882:903	a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	882:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	59	theme	extract	897:903	arg1	%					877:877	2%	876:877	2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	876:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	59	theme	extract	897:903	arg1	PE					906:907	PE	906:907	PE	906:907	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	59	theme	extract	897:903	arg1	%					866:866	0.5%	863:866	0.5%	863:866	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	59	theme	extract	897:903	arg1	%					870:870	1%	869:870	1%	869:870	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	59	theme	extract	897:903	arg1	VFP					855:857	10% powdered VFP	842:857	10% powdered VFP	842:857	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	59	theme	extract	897:903	arg1	gallate					833:839	0.1% methyl gallate	821:839	0.1% methyl gallate	821:839	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	4	60	theme	experimental	615:626	arg1	study					628:632	an interventional experimental study	597:632	an interventional experimental study in mice	597:640	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	6	61	theme	%	1106:1106	arg1	VFP					1117:1119	10% powdered VFP	1104:1119	10% powdered VFP	1104:1119	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	3	62	theme	antioxidant	477:487	arg1	activities					511:520	antioxidant and anti-inflammatory activities	477:520	antioxidant and anti-inflammatory activities with potential anti-obesity effects	477:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	7	63	theme	insulin	1272:1278	arg1	secretion					1280:1288	glucose-stimulated insulin secretion	1253:1288	glucose-stimulated insulin secretion in INS-1E cells	1253:1304	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	2	64	from	polyphenols	311:321	arg1	rich					303:306	rich	303:306	rich	303:306	Alternative vegetable sources rich in polyphenols are known to prevent or delay the progression of metabolic abnormalities during obesity.
37175691	9	65	theme	insulin	1703:1709	arg1	secretion					1711:1719	insulin secretion	1703:1719	insulin secretion	1703:1719	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	4	66	theme	in	575:576	arg1	preventive					583:592	an in vivo preventive	572:592	an in vivo preventive	572:592	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	3	67	theme	anti-obesity	537:548	arg1	effects					550:556	potential anti-obesity effects	527:556	potential anti-obesity effects	527:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	7	68	theme	polyphenolic	1228:1239	arg1	extract					1241:1247	Vachellia farnesiana polyphenolic extract	1207:1247	Vachellia farnesiana polyphenolic extract	1207:1247	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	68	theme	polyphenolic	1228:1239	arg1	VFPE					1201:1204	a VFPE	1199:1204	a VFPE (Vachellia farnesiana polyphenolic extract)	1199:1248	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	8	69	theme	whole-body	1434:1443	arg1	expenditure					1452:1462	whole-body energy expenditure	1434:1462	whole-body energy expenditure	1434:1462	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	6	70	theme	high-fat	1085:1092	arg1	diet					1094:1097	a high-fat diet	1083:1097	a high-fat diet with 10% powdered VFP for ten additional weeks	1083:1144	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	5	71	theme	0.1	821:823	arg1	%					824:824	%	824:824	%	824:824	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	7	72	theme	Vachellia	1207:1215	arg1	extract					1241:1247	Vachellia farnesiana polyphenolic extract	1207:1247	Vachellia farnesiana polyphenolic extract	1207:1247	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	72	theme	Vachellia	1207:1215	arg1	VFPE					1201:1204	a VFPE	1199:1204	a VFPE (Vachellia farnesiana polyphenolic extract)	1199:1248	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	3	73	with	polyphenols	460:470	arg1	activities					511:520	antioxidant and anti-inflammatory activities	477:520	antioxidant and anti-inflammatory activities with potential anti-obesity effects	477:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	2	74	theme	rich	303:306	arg1	sources					295:301	Alternative vegetable sources	273:301	Alternative vegetable sources rich in polyphenols	273:321	Alternative vegetable sources rich in polyphenols are known to prevent or delay the progression of metabolic abnormalities during obesity.
37175691	5	75	theme	methyl	826:831	arg1	gallate					833:839	0.1% methyl gallate	821:839	0.1% methyl gallate	821:839	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	7	76	theme	VFPE	1201:1204	arg1	effect					1189:1194	the effect	1185:1194	the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes	1185:1407	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	2	77	theme	vegetable	285:293	arg1	sources					295:301	Alternative vegetable sources	273:301	Alternative vegetable sources rich in polyphenols	273:321	Alternative vegetable sources rich in polyphenols are known to prevent or delay the progression of metabolic abnormalities during obesity.
37175691	8	78	theme	hepatic	1541:1547	arg1	steatosis					1549:1557	hepatic steatosis	1541:1557	hepatic steatosis	1541:1557	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	5	79	theme	high-fat	789:796	arg1	diet					798:801	a high-fat diet	787:801	a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	787:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	9	80	theme	isolated	1746:1753	arg1	naringenin					1765:1774	naringenin	1765:1774	naringenin	1765:1774	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	80	theme	isolated	1746:1753	arg1	gallate					1787:1793	methyl gallate	1780:1793	methyl gallate	1780:1793	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	80	theme	isolated	1746:1753	arg1	compounds					1755:1763	its isolated compounds	1742:1763	its isolated compounds naringenin and methyl gallate	1742:1793	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	4	81	with	study	628:632	arg1	types					687:691	different cell types	672:691	different cell types	672:691	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	7	82	from	secretion	1280:1288	arg1	cells					1300:1304	INS-1E cells	1293:1304	INS-1E cells	1293:1304	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	82	from	secretion	1280:1288	arg1	activity					1358:1365	mitochondrial activity	1344:1365	mitochondrial activity in primary hepatocytes and C2C12 myotubes	1344:1407	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	5	83	theme	male	719:722	arg1	mice					732:735	male C57BL/6 mice	719:735	male C57BL/6 mice	719:735	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	4	84	dep	in	575:576	arg1	vivo					578:581	vivo	578:581	vivo	578:581	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	8	85	theme	kidney	1564:1569	arg1	damage					1571:1576	kidney damage	1564:1576	kidney damage	1564:1576	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	9	86	theme	methyl	1780:1785	arg1	gallate					1787:1793	methyl gallate	1780:1793	methyl gallate	1780:1793	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	86	theme	methyl	1780:1785	arg1	compounds					1755:1763	its isolated compounds	1742:1763	its isolated compounds naringenin and methyl gallate	1742:1793	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	7	87	theme	C2C12	1394:1398	arg1	myotubes					1400:1407	C2C12 myotubes	1394:1407	C2C12 myotubes	1394:1407	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	10	88	from	expenditure	2042:2052	arg1	secretion					2113:2121	insulin secretion	2105:2121	insulin secretion	2105:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	4	89	from	study	628:632	arg1	mice					637:640	mice	637:640	mice	637:640	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	5	90	theme	Vachellia	928:936	arg1	pods					949:952	Vachellia farnesiana pods	928:952	Vachellia farnesiana pods	928:952	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	90	theme	Vachellia	928:936	arg1	VFP					923:925	VFP	923:925	VFP (Vachellia farnesiana pods)	923:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	8	91	theme	immunomodulatory	1587:1602	arg1	effects					1604:1610	exerted immunomodulatory effects	1579:1610	exerted immunomodulatory effects	1579:1610	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	5	92	attach	derived	910:916	arg1	pods					949:952	Vachellia farnesiana pods	928:952	Vachellia farnesiana pods	928:952	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	92	attach	derived	910:916	arg2	PE					906:907	PE	906:907	PE	906:907	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	92	attach	derived	910:916	arg2	extract					897:903	a polyphenolic extract	882:903	a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	882:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	92	attach	derived	910:916	arg1	VFP					923:925	VFP	923:925	VFP (Vachellia farnesiana pods)	923:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	7	93	theme	in	1154:1155	arg1	studies					1163:1169	the in vitro studies	1150:1169	the in vitro studies	1150:1169	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	4	94	theme	cell	682:685	arg1	types					687:691	different cell types	672:691	different cell types	672:691	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	5	95	theme	C57BL/6	724:730	arg1	mice					732:735	male C57BL/6 mice	719:735	male C57BL/6 mice	719:735	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	0	96	theme	Immunomodulatory	76:91	arg1	Effects					135:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	5	97	theme	%	844:844	arg1	VFP					855:857	10% powdered VFP	842:857	10% powdered VFP	842:857	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	9	98	theme	primary	1831:1837	arg1	hepatocytes					1839:1849	primary hepatocytes	1831:1849	primary hepatocytes	1831:1849	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	10	99	from	function	2078:2085	arg1	secretion					2113:2121	insulin secretion	2105:2121	insulin secretion	2105:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	0	100	theme	Metabolic	94:102	arg1	Effects					135:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	8	101	theme	reshaped	1617:1624	arg1	composition					1647:1657	reshaped fecal gut microbiota composition	1617:1657	reshaped fecal gut microbiota composition	1617:1657	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	7	102	theme	mitochondrial	1344:1356	arg1	activity					1358:1365	mitochondrial activity	1344:1365	mitochondrial activity in primary hepatocytes and C2C12 myotubes	1344:1407	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	10	103	theme	energy	2035:2040	arg1	expenditure					2042:2052	increased energy expenditure	2025:2052	increased energy expenditure	2025:2052	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	0	104	theme	Renoprotective	105:118	arg1	Effects					135:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	7	105	dep	in	1154:1155	arg1	vitro					1157:1161	vitro	1157:1161	vitro	1157:1161	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	106	from	activity	1819:1826	arg1	hepatocytes					1839:1849	primary hepatocytes	1831:1849	primary hepatocytes	1831:1849	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	9	106	from	activity	1819:1826	arg1	myotubes					1861:1868	C2C12 myotubes	1855:1868	C2C12 myotubes	1855:1868	VFPE decreased insulin secretion in INS-1E cells, and its isolated compounds naringenin and methyl gallate increased mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	0	107	theme	farnesiana	10:19	arg1	Pods					21:24	Vachellia farnesiana Pods	0:24	Vachellia farnesiana Pods	0:24	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	10	108	from	reduction	2092:2100	arg1	secretion					2113:2121	insulin secretion	2105:2121	insulin secretion	2105:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	10	109	theme	mitochondrial	2064:2076	arg1	function					2078:2085	improved mitochondrial function	2055:2085	improved mitochondrial function	2055:2085	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	1	110	theme	microbiota	251:260	arg1	dysbiosis					262:270	gut microbiota dysbiosis	247:270	gut microbiota dysbiosis	247:270	Obesity causes systemic inflammation, hepatic and renal damage, as well as gut microbiota dysbiosis.
37175691	8	111	theme	gut	1632:1634	arg1	composition					1647:1657	reshaped fecal gut microbiota composition	1617:1657	reshaped fecal gut microbiota composition	1617:1657	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
37175691	10	112	from	inflammation	1918:1929	arg1	mice					1984:1987	mice	1984:1987	mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion	1984:2121	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	3	113	theme	potent	443:448	arg1	source					450:455	a potent source	441:455	a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects	441:556	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	3	113	theme	potent	443:448	arg1	farnesiana					422:431	Vachellia farnesiana	412:431	Vachellia farnesiana (VF)	412:436	Vachellia farnesiana (VF) is a potent source of polyphenols with antioxidant and anti-inflammatory activities with potential anti-obesity effects.
37175691	0	114	theme	Polyphenolic	31:42	arg1	Extract					44:50	a Polyphenolic Extract	29:50	a Polyphenolic Extract	29:50	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	4	115	dep	in	646:647	arg1	vitro					649:653	vitro	649:653	vitro	649:653	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	4	116	with	experiments	655:665	arg1	types					687:691	different cell types	672:691	different cell types	672:691	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	2	117	theme	abnormalities	382:394	arg1	progression					357:367	the progression	353:367	the progression of metabolic abnormalities during obesity	353:409	Alternative vegetable sources rich in polyphenols are known to prevent or delay the progression of metabolic abnormalities during obesity.
37175691	7	118	theme	methyl	1326:1331	arg1	gallate					1333:1339	methyl gallate	1326:1339	methyl gallate	1326:1339	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	0	119	theme	Prebiotic	125:133	arg1	Effects					135:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects	76:141	Vachellia farnesiana Pods or a Polyphenolic Extract Derived from Them Exert Immunomodulatory, Metabolic, Renoprotective, and Prebiotic Effects in Mice Fed a High-Fat Diet.
37175691	7	120	theme	naringenin	1312:1321	arg1	effect					1189:1194	the effect	1185:1194	the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes	1185:1407	In the in vitro studies, we evaluated the effect of a VFPE (Vachellia farnesiana polyphenolic extract) on glucose-stimulated insulin secretion in INS-1E cells or of naringenin or methyl gallate on mitochondrial activity in primary hepatocytes and C2C12 myotubes.
37175691	6	121	theme	intervention	976:987	arg1	study					989:993	the intervention study	972:993	the intervention study	972:993	In the intervention study, two groups of mice were fed for 14 weeks with a high-fat diet and then one switched to a high-fat diet with 10% powdered VFP for ten additional weeks.
37175691	4	122	theme	interventional	600:613	arg1	study					628:632	an interventional experimental study	597:632	an interventional experimental study in mice	597:640	We performed an in vivo preventive or an interventional experimental study in mice and in vitro experiments with different cell types.
37175691	10	123	theme	increased	2025:2033	arg1	expenditure					2042:2052	increased energy expenditure	2025:2052	increased energy expenditure	2025:2052	In conclusion VFP or a VFPE prevented systemic inflammation, insulin resistance, and hepatic and renal damage in mice fed a high-fat diet associated with increased energy expenditure, improved mitochondrial function, and reduction in insulin secretion.
37175691	5	124	theme	polyphenolic	884:895	arg1	PE					906:907	PE	906:907	PE	906:907	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	5	124	theme	polyphenolic	884:895	arg1	extract					897:903	a polyphenolic extract	882:903	a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods)	882:953	In the preventive study, male C57BL/6 mice were fed with a Control diet, a high-fat diet, or a high-fat diet containing either 0.1% methyl gallate, 10% powdered VFP, or 0.5%, 1%, or 2% of a polyphenolic extract (PE) derived from VFP (Vachellia farnesiana pods) for 14 weeks.
37175691	8	125	theme	skeletal	1494:1501	arg1	muscle					1503:1508	skeletal muscle	1494:1508	skeletal muscle	1494:1508	VFP or a VFPE increased whole-body energy expenditure and mitochondrial activity in skeletal muscle; prevented insulin resistance, hepatic steatosis, and kidney damage; exerted immunomodulatory effects; and reshaped fecal gut microbiota composition in mice fed a high-fat diet.
36011261	7	0	theme	Quality	1129:1135	arg1	analysis					1137:1144	Quality analysis	1129:1144	Quality analysis	1129:1144	Quality analysis showed that the low salinity could increase the starch content and influence the amylopectin biosynthesis.
36011261	10	1	theme	composition	1530:1540	arg1	level					1554:1558	the composition and content level	1526:1558	the composition and content level of the purple sweetpotato	1526:1584	Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato.
36011261	4	2	theme	high	846:849	arg1	salinity					851:858	high salinity	846:858	high salinity	846:858	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	10	3	theme	low	1500:1502	arg1	salinity					1504:1511	the low salinity	1496:1511	the low salinity	1496:1511	Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato.
36011261	8	4	theme	substance	1293:1301	arg1	accumulation					1303:1314	substance accumulation	1293:1314	substance accumulation	1293:1314	It suggested that low salinity promotes substance accumulation.
36011261	9	5	dep	salinity	1322:1329	arg1	increase					1337:1344	increase	1337:1344	could increase the anthocyanins biosynthesis and low salinity	1331:1391	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	2	6	theme	mmol/L	331:336	arg1	NaCl					338:341	34 mmol/L NaCl	328:341	34 mmol/L NaCl	328:341	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	2	6	theme	mmol/L	331:336	arg1	salinity					318:325	low salinity	314:325	low salinity (34 mmol/L NaCl)	314:342	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	0	7	theme	Salinity	107:114	arg1	Levels					116:121	Different Salinity Levels	97:121	Different Salinity Levels	97:121	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	0	8	from	Evaluation	0:9	arg1	SweetPotato					79:89	Purple SweetPotato	72:89	Purple SweetPotato under Different Salinity Levels	72:121	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	10	9	theme	content	1546:1552	arg1	level					1554:1558	the composition and content level	1526:1558	the composition and content level of the purple sweetpotato	1526:1584	Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato.
36011261	4	10	theme	other	749:753	arg1	ions					755:758	other ions	749:758	other ions	749:758	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	3	11	theme	catabolic	550:558	arg1	pathways					589:596	the significant pathways	573:596	the significant pathways under the salinity stress	573:622	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	11	theme	catabolic	550:558	arg1	glycometabolism					484:498	glycometabolism	484:498	glycometabolism	484:498	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	11	theme	catabolic	550:558	arg1	process					560:566	the starch catabolic process	539:566	the starch catabolic process	539:566	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	11	theme	catabolic	550:558	arg1	biosynthesis					522:533	secondary metabolite biosynthesis	501:533	secondary metabolite biosynthesis	501:533	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	9	12	theme	phenolic	1421:1428	arg1	acid					1430:1433	phenolic acid	1421:1433	phenolic acid	1421:1433	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	0	13	theme	Different	97:105	arg1	Levels					116:121	Different Salinity Levels	97:121	Different Salinity Levels	97:121	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	9	14	contain	had	1393:1395	arg1	salinity					1322:1329	High salinity	1317:1329	High salinity could increase the anthocyanins biosynthesis and low salinity	1317:1391	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	9	14	contain	had	1393:1395	arg2	impact					1411:1416	a significant impact	1397:1416	a significant impact	1397:1416	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	5	15	theme	related	938:944	arg1	expression					951:960	their related gene expression	932:960	their related gene expression	932:960	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	10	16	dep	gene	1462:1465	arg1	prove					1490:1494	prove	1490:1494	prove the low salinity	1490:1511	Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato.
36011261	11	17	from	application	1678:1688	arg1	planting					1712:1719	purple sweetpotato planting	1693:1719	purple sweetpotato planting	1693:1719	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	8	18	theme	low	1271:1273	arg1	salinity					1275:1282	low salinity	1271:1282	low salinity	1271:1282	It suggested that low salinity promotes substance accumulation.
36011261	5	19	theme	antioxidant	908:918	arg1	enzymes					920:926	antioxidant enzymes	908:926	antioxidant enzymes	908:926	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	4	20	dep	tolerance	806:814	arg1	response					777:784	response	777:784	response	777:784	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	3	21	theme	metabolite	511:520	arg1	pathways					589:596	the significant pathways	573:596	the significant pathways under the salinity stress	573:622	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	21	theme	metabolite	511:520	arg1	glycometabolism					484:498	glycometabolism	484:498	glycometabolism	484:498	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	21	theme	metabolite	511:520	arg1	process					560:566	the starch catabolic process	539:566	the starch catabolic process	539:566	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	21	theme	metabolite	511:520	arg1	biosynthesis					522:533	secondary metabolite biosynthesis	501:533	secondary metabolite biosynthesis	501:533	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	1	22	theme	abiotic	173:179	arg1	stresses					181:188	the principal abiotic stresses	159:188	the principal abiotic stresses affecting crop yield	159:209	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	7	23	theme	low	1162:1164	arg1	salinity					1166:1173	the low salinity	1158:1173	the low salinity	1158:1173	Quality analysis showed that the low salinity could increase the starch content and influence the amylopectin biosynthesis.
36011261	5	24	theme	low	878:880	arg1	salinity					882:889	low salinity	878:889	low salinity	878:889	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	6	25	theme	starch	1111:1116	arg1	synthesis					1118:1126	starch synthesis	1111:1126	starch synthesis	1111:1126	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	3	26	dep	indicated	469:477	arg1	Using					407:411	Using	407:411	Using transcriptomics and metabolomics to profile	407:455	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	1	27	theme	stresses	181:188	arg1	stresses					181:188	the principal abiotic stresses	159:188	the principal abiotic stresses affecting crop yield	159:209	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	1	27	theme	stresses	181:188	arg1	one					152:154	one	152:154	one	152:154	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	9	28	theme	High	1317:1320	arg1	salinity					1322:1329	High salinity	1317:1329	High salinity could increase the anthocyanins biosynthesis and low salinity	1317:1391	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	4	29	theme	purple	654:659	arg1	sweetpotato					661:671	purple sweetpotato	654:671	purple sweetpotato	654:671	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	11	30	theme	sweetpotato	1700:1710	arg1	planting					1712:1719	purple sweetpotato planting	1693:1719	purple sweetpotato planting	1693:1719	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	4	31	theme	Na+	736:738	arg1	concentration					760:772	the cellular Na+, K+, and other ions concentration	723:772	the cellular Na+, K+, and other ions concentration	723:772	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	2	32	theme	high	348:351	arg1	salinity					353:360	high salinity	348:360	high salinity (85 mmol/L)	348:372	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	2	32	theme	high	348:351	arg1	mmol/L					366:371	85 mmol/L	363:371	85 mmol/L	363:371	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	0	33	theme	Coping	28:33	arg1	Strategies					35:44	Physiological Coping Strategies	14:44	Physiological Coping Strategies	14:44	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	6	34	dep	content	1068:1074	arg1	the					1064:1066	the	1064:1066	the	1064:1066	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	3	35	theme	starch	543:548	arg1	pathways					589:596	the significant pathways	573:596	the significant pathways under the salinity stress	573:622	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	35	theme	starch	543:548	arg1	glycometabolism					484:498	glycometabolism	484:498	glycometabolism	484:498	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	35	theme	starch	543:548	arg1	process					560:566	the starch catabolic process	539:566	the starch catabolic process	539:566	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	35	theme	starch	543:548	arg1	biosynthesis					522:533	secondary metabolite biosynthesis	501:533	secondary metabolite biosynthesis	501:533	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	5	36	theme	high	982:985	arg1	level					987:991	a high level	980:991	a high level	980:991	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	10	37	theme	expression	1467:1476	arg1	levels					1478:1483	expression levels	1467:1483	expression levels	1467:1483	Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato.
36011261	2	38	theme	purple	387:392	arg1	sweetpotato					394:404	purple sweetpotato	387:404	purple sweetpotato	387:404	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	1	39	theme	crop	284:287	arg1	quality					289:295	crop quality	284:295	crop quality	284:295	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	4	40	theme	concentration	760:772	arg1	regulation					709:718	the regulation	705:718	the regulation of the cellular Na+, K+, and other ions concentration	705:772	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	1	41	theme	principal	163:171	arg1	stresses					181:188	the principal abiotic stresses	159:188	the principal abiotic stresses affecting crop yield	159:209	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	4	42	theme	Further	625:631	arg1	research					633:640	Further research	625:640	Further research	625:640	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	11	43	used	used	1654:1657	arg2	concentration					1625:1637	an appropriate concentration	1610:1637	an appropriate concentration of NaCl	1610:1645	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	11	43	used	used	1654:1657	arg2	elicitor					1665:1672	an elicitor	1662:1672	an elicitor for application in purple sweetpotato planting	1662:1719	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	6	44	theme	genes	1094:1098	arg1	content					1068:1074	content	1068:1074	content	1068:1074	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	6	44	theme	genes	1094:1098	arg1	composition					1041:1051	the monosaccharide composition	1022:1051	the monosaccharide composition as well as the content and regulation of genes related to starch synthesis	1022:1126	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	6	44	theme	genes	1094:1098	arg1	regulation					1080:1089	regulation	1080:1089	regulation	1080:1089	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	10	45	theme	sweetpotato	1574:1584	arg1	level					1554:1558	the composition and content level	1526:1558	the composition and content level of the purple sweetpotato	1526:1584	Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato.
36011261	4	46	theme	salinity	797:804	arg1	tolerance					806:814	the low salinity tolerance	789:814	the low salinity tolerance	789:814	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	1	47	theme	crop	200:203	arg1	yield					205:209	crop yield	200:209	crop yield	200:209	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	0	48	theme	Strategies	35:44	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.	0:122	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	9	49	theme	low	1380:1382	arg1	salinity					1384:1391	low salinity	1380:1391	low salinity	1380:1391	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	9	49	theme	low	1380:1382	arg1	anthocyanins					1350:1361	the anthocyanins biosynthesis and low salinity	1346:1391	the anthocyanins biosynthesis and low salinity	1346:1391	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	4	50	theme	low	793:795	arg1	tolerance					806:814	the low salinity tolerance	789:814	the low salinity tolerance	789:814	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	1	51	theme	suitable	214:221	arg1	concentration					223:235	a suitable concentration	212:235	a suitable concentration of NaCl	212:243	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	1	51	theme	suitable	214:221	arg1	useful					262:267	useful	262:267	useful	262:267	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	0	52	theme	Substances	58:67	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.	0:122	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	10	53	theme	purple	1567:1572	arg1	sweetpotato					1574:1584	the purple sweetpotato	1563:1584	the purple sweetpotato	1563:1584	Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato.
36011261	6	54	theme	low	998:1000	arg1	salinity					1002:1009	The low salinity	994:1009	The low salinity	994:1009	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	9	55	dep	anthocyanins	1350:1361	arg1	biosynthesis					1363:1374	biosynthesis	1363:1374	biosynthesis	1363:1374	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	9	55	dep	anthocyanins	1350:1361	arg1	salinity					1384:1391	low salinity	1380:1391	low salinity	1380:1391	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	9	55	dep	anthocyanins	1350:1361	arg1	anthocyanins					1350:1361	the anthocyanins biosynthesis and low salinity	1346:1391	the anthocyanins biosynthesis and low salinity	1346:1391	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
36011261	6	56	theme	related	1100:1106	arg1	genes					1094:1098	genes	1094:1098	genes related to starch synthesis	1094:1126	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	0	57	theme	Quality	50:56	arg1	Substances					58:67	Quality Substances	50:67	Quality Substances	50:67	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	7	58	theme	starch	1194:1199	arg1	content					1201:1207	the starch content	1190:1207	the starch content	1190:1207	Quality analysis showed that the low salinity could increase the starch content and influence the amylopectin biosynthesis.
36011261	3	59	theme	significant	577:587	arg1	pathways					589:596	the significant pathways	573:596	the significant pathways under the salinity stress	573:622	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	59	theme	significant	577:587	arg1	glycometabolism					484:498	glycometabolism	484:498	glycometabolism	484:498	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	59	theme	significant	577:587	arg1	process					560:566	the starch catabolic process	539:566	the starch catabolic process	539:566	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	59	theme	significant	577:587	arg1	biosynthesis					522:533	secondary metabolite biosynthesis	501:533	secondary metabolite biosynthesis	501:533	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	1	60	theme	salinity	133:140	arg1	stress					142:147	salinity stress	133:147	salinity stress	133:147	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	3	61	theme	salinity	608:615	arg1	stress					617:622	the salinity stress	604:622	the salinity stress	604:622	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	7	62	theme	amylopectin	1227:1237	arg1	biosynthesis					1239:1250	the amylopectin biosynthesis	1223:1250	the amylopectin biosynthesis	1223:1250	Quality analysis showed that the low salinity could increase the starch content and influence the amylopectin biosynthesis.
36011261	4	63	theme	cellular	727:734	arg1	concentration					760:772	the cellular Na+, K+, and other ions concentration	723:772	the cellular Na+, K+, and other ions concentration	723:772	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	5	64	theme	enzymes	920:926	arg1	Meanwhile					861:869	Meanwhile	861:869	Meanwhile	861:869	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	5	64	theme	enzymes	920:926	arg1	expression					951:960	their related gene expression	932:960	their related gene expression	932:960	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	5	64	theme	enzymes	920:926	arg1	activity					896:903	the activity	892:903	the activity of antioxidant enzymes	892:926	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	4	65	theme	ions	755:758	arg1	concentration					760:772	the cellular Na+, K+, and other ions concentration	723:772	the cellular Na+, K+, and other ions concentration	723:772	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	11	66	theme	purple	1693:1698	arg1	planting					1712:1719	purple sweetpotato planting	1693:1719	purple sweetpotato planting	1693:1719	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	6	67	theme	monosaccharide	1026:1039	arg1	composition					1041:1051	the monosaccharide composition	1022:1051	the monosaccharide composition as well as the content and regulation of genes related to starch synthesis	1022:1126	The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis.
36011261	4	68	theme	K+	741:742	arg1	concentration					760:772	the cellular Na+, K+, and other ions concentration	723:772	the cellular Na+, K+, and other ions concentration	723:772	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	2	69	theme	low	314:316	arg1	NaCl					338:341	34 mmol/L NaCl	328:341	34 mmol/L NaCl	328:341	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	2	69	theme	low	314:316	arg1	salinity					318:325	low salinity	314:325	low salinity (34 mmol/L NaCl)	314:342	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	0	70	theme	Purple	72:77	arg1	SweetPotato					79:89	Purple SweetPotato	72:89	Purple SweetPotato under Different Salinity Levels	72:121	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	5	71	theme	gene	946:949	arg1	expression					951:960	their related gene expression	932:960	their related gene expression	932:960	Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level.
36011261	11	72	theme	appropriate	1613:1623	arg1	concentration					1625:1637	an appropriate concentration	1610:1637	an appropriate concentration of NaCl	1610:1645	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	11	72	theme	appropriate	1613:1623	arg1	elicitor					1665:1672	an elicitor	1662:1672	an elicitor for application in purple sweetpotato planting	1662:1719	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	4	73	theme	related	694:700	arg1	genes					688:692	genes	688:692	genes related to the regulation of the cellular Na+, K+, and other ions concentration	688:772	Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity.
36011261	2	74	used	used	309:312	arg2	study					303:307	This study	298:307	This study	298:307	This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato.
36011261	1	75	theme	NaCl	240:243	arg1	concentration					223:235	a suitable concentration	212:235	a suitable concentration of NaCl	212:243	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	1	75	theme	NaCl	240:243	arg1	useful					262:267	useful	262:267	useful	262:267	Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality.
36011261	11	76	theme	NaCl	1642:1645	arg1	concentration					1625:1637	an appropriate concentration	1610:1637	an appropriate concentration of NaCl	1610:1645	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	11	76	theme	NaCl	1642:1645	arg1	elicitor					1665:1672	an elicitor	1662:1672	an elicitor for application in purple sweetpotato planting	1662:1719	This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.
36011261	0	77	theme	Physiological	14:26	arg1	Strategies					35:44	Physiological Coping Strategies	14:44	Physiological Coping Strategies	14:44	Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels.
36011261	3	78	theme	secondary	501:509	arg1	pathways					589:596	the significant pathways	573:596	the significant pathways under the salinity stress	573:622	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	78	theme	secondary	501:509	arg1	glycometabolism					484:498	glycometabolism	484:498	glycometabolism	484:498	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	78	theme	secondary	501:509	arg1	process					560:566	the starch catabolic process	539:566	the starch catabolic process	539:566	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	3	78	theme	secondary	501:509	arg1	biosynthesis					522:533	secondary metabolite biosynthesis	501:533	secondary metabolite biosynthesis	501:533	Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress.
36011261	9	79	theme	significant	1399:1409	arg1	impact					1411:1416	a significant impact	1397:1416	a significant impact	1397:1416	High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol.
35879170	13	0	from	effect	2718:2723	arg1	ARS					2732:2734	the ARS	2728:2734	the ARS of B1	2728:2740	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	13	0	from	effect	2718:2723	arg1	ARS					2771:2773	the ARS	2767:2773	the ARS of B9	2767:2779	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	7	1	theme	detergent	1597:1605	arg1	fiber					1607:1611	rumen digestible neutral detergent fiber	1572:1611	rumen digestible neutral detergent fiber (NDF)	1572:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	1	theme	detergent	1597:1605	arg1	NDF					1614:1616	NDF	1614:1616	NDF	1614:1616	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	2	theme	B3	2645:2646	arg1	ARS					2634:2636	the ARS	2630:2636	the ARS of B2, B3, and B6	2630:2654	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	4	3	theme	empirical	951:959	arg1	models					961:966	empirical models	951:966	empirical models	951:966	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	15	4	theme	dairy	3030:3034	arg1	cow					3036:3038	the dairy cow	3026:3038	the dairy cow	3026:3038	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	12	5	theme	B1	2511:2512	arg1	ARS					2504:2506	the ARS	2500:2506	the ARS of B1, B2, B3, and B6	2500:2528	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	1	6	theme	modern	215:220	arg1	cow					228:230	the modern dairy cow	211:230	the modern dairy cow	211:230	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	10	7	theme	Concordance	2165:2175	arg1	coefficient					2189:2199	Concordance correlation coefficient	2165:2199	Concordance correlation coefficient	2165:2199	Concordance correlation coefficient was used to evaluate the models on the developmental data set due to data scarcity.
35879170	7	8	theme	digestible	1578:1587	arg1	fiber					1607:1611	rumen digestible neutral detergent fiber	1572:1611	rumen digestible neutral detergent fiber (NDF)	1572:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	8	theme	digestible	1578:1587	arg1	NDF					1614:1616	NDF	1614:1616	NDF	1614:1616	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	9	theme	molar	1726:1730	arg1	proportions					1732:1742	molar proportions	1726:1742	molar proportions	1726:1742	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	8	10	theme	DM	1985:1986	arg1	g/kg					1977:1980	g/kg	1977:1980	g/kg of DM	1977:1986	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	7	11	dep	acetate	1682:1688	arg1	VFA					1750:1752	VFA	1750:1752	VFA	1750:1752	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	11	dep	acetate	1682:1688	arg1	%					1745:1745	%	1745:1745	% of VFA	1745:1752	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	11	dep	acetate	1682:1688	arg1	proportions					1732:1742	molar proportions	1726:1742	molar proportions	1726:1742	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	3	12	theme	complex	625:631	arg1	impact					633:638	their complex impact	619:638	their complex impact on the metabolism of dairy cows	619:670	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	5	13	theme	post	1273:1276	arg1	observations					1174:1185	340 individual cow observations	1155:1185	340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12)	1155:1342	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	13	theme	post	1273:1276	arg1	hoc					1278:1280	post hoc	1273:1280	post hoc	1273:1280	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	15	14	theme	ruminal	2970:2976	arg1	environment					2978:2988	ruminal environment	2970:2988	ruminal environment	2970:2988	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	7	15	theme	variables	1554:1562	arg1	pH					1761:1762	mean pH	1756:1762	mean pH	1756:1762	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	butyrate					1703:1710	butyrate	1703:1710	butyrate	1703:1710	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	starch					1623:1628	starch	1623:1628	starch	1623:1628	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	acids					1665:1669	total volatile fatty acids	1644:1669	total volatile fatty acids (VFA, mM)	1644:1679	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	valerate					1717:1724	valerate	1717:1724	valerate	1717:1724	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	acetate					1682:1688	acetate	1682:1688	acetate	1682:1688	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	[mg/kg					1511:1516	B-vitamin dietary concentration [mg/kg	1479:1516	B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF)	1479:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	intake					1459:1464	dry matter intake	1448:1464	dry matter intake (DMI, kg/d)	1448:1476	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	propionate					1691:1700	propionate	1691:1700	propionate	1691:1700	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	rates					1780:1784	fractional rates	1769:1784	fractional rates of degradation of NDF and starch (%/h)	1769:1823	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	VFA					1672:1674	VFA	1672:1674	VFA	1672:1674	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	15	theme	variables	1554:1562	arg1	DMI					1467:1469	DMI	1467:1469	DMI	1467:1469	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	8	16	theme	dietary-level	1859:1871	arg1	DMI					1899:1901	DMI	1899:1901	DMI	1899:1901	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	16	theme	dietary-level	1859:1871	arg1	starch					1951:1956	starch	1951:1956	starch	1951:1956	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	16	theme	dietary-level	1859:1871	arg1	NDF					2000:2002	forage NDF	1993:2002	forage NDF (g/kg of DM)	1993:2015	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	16	theme	dietary-level	1859:1871	arg1	concentration					1922:1934	B-vitamin dietary concentration	1904:1934	B-vitamin dietary concentration (mg/kg of DM)	1904:1948	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	16	theme	dietary-level	1859:1871	arg1	variables					1881:1889	dietary-level driving variables	1859:1889	dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM)	1859:2015	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	7	17	dep	DMI	1467:1469	arg1	kg/d					1472:1475	kg/d	1472:1475	kg/d	1472:1475	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	3	18	from	impact	633:638	arg1	metabolism					647:656	the metabolism	643:656	the metabolism of dairy cows	643:670	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	3	19	theme	dairy	661:665	arg1	cows					667:670	dairy cows	661:670	dairy cows	661:670	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	15	20	theme	B	3012:3012	arg1	vitamins					3014:3021	B vitamins	3012:3021	B vitamins	3012:3021	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	2	21	theme	dietary	487:493	arg1	intake					495:500	dietary intake	487:500	dietary intake	487:500	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	8	22	dep	starch	1951:1956	arg1	g/kg					1977:1980	g/kg	1977:1980	g/kg of DM	1977:1986	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	1	23	theme	existing	166:173	arg1	estimates					175:183	existing estimates	166:183	existing estimates of the B-vitamin needs of the modern dairy cow	166:230	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	7	24	theme	dry	1448:1450	arg1	DMI					1467:1469	DMI	1467:1469	DMI	1467:1469	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	24	theme	dry	1448:1450	arg1	intake					1459:1464	dry matter intake	1448:1464	dry matter intake (DMI, kg/d)	1448:1476	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	25	theme	B1	2739:2740	arg1	ARS					2732:2734	the ARS	2728:2734	the ARS of B1	2728:2740	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	4	26	theme	B-vitamin	1011:1019	arg1	supply					1021:1026	true B-vitamin supply	1006:1026	true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS)	1006:1098	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	15	27	from	development	3058:3068	arg1	future					3108:3113	future	3108:3113	future	3108:3113	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	5	28	dep	vitamins	1309:1316	arg1	B1					1319:1320	B1	1319:1320	B1	1319:1320	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	28	dep	vitamins	1309:1316	arg1	B6					1331:1332	B6	1331:1332	B6	1331:1332	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	28	dep	vitamins	1309:1316	arg1	B9					1335:1336	B9	1335:1336	B9	1335:1336	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	28	dep	vitamins	1309:1316	arg1	B2					1323:1324	B2	1323:1324	B2	1323:1324	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	28	dep	vitamins	1309:1316	arg1	B3					1327:1328	B3	1327:1328	B3	1327:1328	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	28	dep	vitamins	1309:1316	arg1	B12					1339:1341	B12	1339:1341	B12	1339:1341	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	8	29	theme	DM	2013:2014	arg1	g/kg					2005:2008	g/kg	2005:2008	g/kg of DM	2005:2014	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	1	30	dep	B-vitamin	192:200	arg1	needs					202:206	needs	202:206	needs of the modern dairy cow	202:230	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	10	31	used	used	2205:2208	arg2	coefficient					2189:2199	Concordance correlation coefficient	2165:2199	Concordance correlation coefficient	2165:2199	Concordance correlation coefficient was used to evaluate the models on the developmental data set due to data scarcity.
35879170	10	32	theme	correlation	2177:2187	arg1	coefficient					2189:2199	Concordance correlation coefficient	2165:2199	Concordance correlation coefficient	2165:2199	Concordance correlation coefficient was used to evaluate the models on the developmental data set due to data scarcity.
35879170	6	33	theme	form	1393:1396	arg1	Equations					1345:1353	Equations	1345:1353	Equations of univariate and multivariate linear form	1345:1396	Equations of univariate and multivariate linear form were considered.
35879170	7	34	theme	Models	1415:1420	arg1	ARS					1433:1435	Models describing ARS	1415:1435	Models describing ARS	1415:1435	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	11	35	theme	better-performing	2315:2331	arg1	models					2333:2338	better-performing models	2315:2338	better-performing models	2315:2338	Overall, modeling ARS yielded better-performing models compared with modeling PRF, and DMI was included in all prediction equations as a scalar variable.
35879170	8	36	theme	dietary	1914:1920	arg1	concentration					1922:1934	B-vitamin dietary concentration	1904:1934	B-vitamin dietary concentration (mg/kg of DM)	1904:1948	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	2	37	theme	vitamins	603:610	arg1	utilization					586:596	utilization	586:596	utilization	586:596	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	2	37	theme	vitamins	603:610	arg1	synthesis					572:580	synthesis	572:580	synthesis	572:580	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	7	38	theme	fractional	1769:1778	arg1	rates					1780:1784	fractional rates	1769:1784	fractional rates of degradation of NDF and starch (%/h)	1769:1823	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	4	39	theme	apparent	1067:1074	arg1	ARS					1095:1097	ARS	1095:1097	ARS	1095:1097	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	4	39	theme	apparent	1067:1074	arg1	synthesis					1084:1092	apparent ruminal synthesis	1067:1092	apparent ruminal synthesis (ARS)	1067:1098	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	6	40	theme	multivariate	1373:1384	arg1	form					1393:1396	univariate and multivariate linear form	1358:1396	univariate and multivariate linear form	1358:1396	Equations of univariate and multivariate linear form were considered.
35879170	7	41	theme	matter	1525:1530	arg1	pH					1761:1762	mean pH	1756:1762	mean pH	1756:1762	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	butyrate					1703:1710	butyrate	1703:1710	butyrate	1703:1710	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	starch					1623:1628	starch	1623:1628	starch	1623:1628	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	acids					1665:1669	total volatile fatty acids	1644:1669	total volatile fatty acids (VFA, mM)	1644:1679	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	valerate					1717:1724	valerate	1717:1724	valerate	1717:1724	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	acetate					1682:1688	acetate	1682:1688	acetate	1682:1688	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	[mg/kg					1511:1516	B-vitamin dietary concentration [mg/kg	1479:1516	B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF)	1479:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	intake					1459:1464	dry matter intake	1448:1464	dry matter intake (DMI, kg/d)	1448:1476	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	propionate					1691:1700	propionate	1691:1700	propionate	1691:1700	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	rates					1780:1784	fractional rates	1769:1784	fractional rates of degradation of NDF and starch (%/h)	1769:1823	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	VFA					1672:1674	VFA	1672:1674	VFA	1672:1674	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	41	theme	matter	1525:1530	arg1	DMI					1467:1469	DMI	1467:1469	DMI	1467:1469	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	42	theme	digestible	2576:2585	arg1	concentration					2591:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	2	43	theme	B-vitamin	431:439	arg1	supply					441:446	"true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply	357:446	"true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply	357:446	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	4	44	theme	postruminal	1040:1050	arg1	PRF					1058:1060	PRF	1058:1060	PRF	1058:1060	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	4	44	theme	postruminal	1040:1050	arg1	flow					1052:1055	postruminal flow	1040:1055	postruminal flow	1040:1055	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	11	45	theme	modeling	2354:2361	arg1	PRF					2363:2365	modeling PRF	2354:2365	modeling PRF	2354:2365	Overall, modeling ARS yielded better-performing models compared with modeling PRF, and DMI was included in all prediction equations as a scalar variable.
35879170	5	46	theme	cow	1170:1172	arg1	observations					1174:1185	340 individual cow observations	1155:1185	340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12)	1155:1342	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	46	theme	cow	1170:1172	arg1	hoc					1278:1280	post hoc	1273:1280	post hoc	1273:1280	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	9	47	theme	slope	2079:2083	arg1	parameters					2085:2094	all significant slope parameters	2063:2094	all significant slope parameters	2063:2094	Equations developed were required to contain all significant slope parameters and contained no significant collinearity between driving variables.
35879170	6	48	theme	univariate	1358:1367	arg1	form					1393:1396	univariate and multivariate linear form	1358:1396	univariate and multivariate linear form	1358:1396	Equations of univariate and multivariate linear form were considered.
35879170	14	49	theme	dietary	2817:2823	arg1	starch					2825:2830	the dietary starch	2813:2830	the dietary starch	2813:2830	In the best prediction models, the dietary starch increased PRF of B1, B2, and B9 but decreased PRF of B12.
35879170	7	50	theme	mean	1756:1759	arg1	pH					1761:1762	mean pH	1756:1762	mean pH	1756:1762	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	3	51	theme	mathematical	735:746	arg1	models					748:753	mathematical models	735:753	mathematical models	735:753	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	0	52	theme	vitamins	70:77	arg1	synthesis					34:42	apparent ruminal synthesis	17:42	apparent ruminal synthesis	17:42	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	0	52	theme	vitamins	70:77	arg1	flow					60:63	postruminal flow	48:63	postruminal flow	48:63	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	15	53	theme	better-defined	3073:3086	arg1	requirements					3088:3099	better-defined requirements	3073:3099	better-defined requirements	3073:3099	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	1	54	theme	past	150:153	arg1	s					162:162	s	162:162	s	162:162	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	1	54	theme	past	150:153	arg1	decade					155:160	the past decade	146:160	the past decade(s)	146:163	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	7	55	theme	concentration	1497:1509	arg1	[mg/kg					1511:1516	B-vitamin dietary concentration [mg/kg	1479:1516	B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF)	1479:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	0	56	from	Meta-analysis	0:12	arg1	cows					88:91	dairy cows	82:91	dairy cows	82:91	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	12	57	contain	had	2475:2477	arg2	effect					2490:2495	a negative effect	2479:2495	a negative effect	2479:2495	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	12	57	contain	had	2475:2477	arg1	concentration					2461:2473	The B-vitamin dietary concentration	2439:2473	The B-vitamin dietary concentration	2439:2473	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	0	58	theme	dairy	82:86	arg1	cows					88:91	dairy cows	82:91	dairy cows	82:91	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	7	59	theme	B-vitamin	1479:1487	arg1	[mg/kg					1511:1516	B-vitamin dietary concentration [mg/kg	1479:1516	B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF)	1479:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	14	60	theme	prediction	2794:2803	arg1	models					2805:2810	the best prediction models	2785:2810	the best prediction models	2785:2810	In the best prediction models, the dietary starch increased PRF of B1, B2, and B9 but decreased PRF of B12.
35879170	5	61	theme	vitamins	1309:1316	arg1	content					1296:1302	content	1296:1302	content of B vitamins (B1, B2, B3, B6, B9, B12)	1296:1342	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	2	62	theme	significant	552:562	arg1	role					564:567	a significant role	550:567	a significant role	550:567	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	4	63	theme	vitamins	930:937	arg1	absence					904:910	the absence	900:910	the absence of supplemental B vitamins	900:937	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	0	64	from	synthesis	34:42	arg1	cows					88:91	dairy cows	82:91	dairy cows	82:91	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	7	65	dep	[mg/kg	1511:1516	arg1	g/kg					1631:1634	g/kg	1631:1634	g/kg of DM	1631:1640	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	12	66	theme	B2	2555:2556	arg1	PRF					2548:2550	the PRF	2544:2550	the PRF of B2 and B9	2544:2563	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	9	67	theme	driving	2146:2152	arg1	variables					2154:2162	driving variables	2146:2162	driving variables	2146:2162	Equations developed were required to contain all significant slope parameters and contained no significant collinearity between driving variables.
35879170	12	68	theme	B-vitamin	2443:2451	arg1	concentration					2461:2473	The B-vitamin dietary concentration	2439:2473	The B-vitamin dietary concentration	2439:2473	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	13	69	contain	had	2605:2607	arg2	effect					2620:2625	a negative effect	2609:2625	a negative effect	2609:2625	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	13	69	contain	had	2605:2607	arg1	concentration					2591:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	10	70	theme	developmental	2240:2252	arg1	data					2254:2257	the developmental data	2236:2257	the developmental data set due to data scarcity	2236:2282	Concordance correlation coefficient was used to evaluate the models on the developmental data set due to data scarcity.
35879170	2	71	theme	"	362:362	arg1	supply					441:446	"true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply	357:446	"true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply	357:446	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	0	72	theme	apparent	17:24	arg1	synthesis					34:42	apparent ruminal synthesis	17:42	apparent ruminal synthesis	17:42	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	7	73	theme	volatile	1650:1657	arg1	VFA					1672:1674	VFA	1672:1674	VFA	1672:1674	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	73	theme	volatile	1650:1657	arg1	acids					1665:1669	total volatile fatty acids	1644:1669	total volatile fatty acids (VFA, mM)	1644:1679	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	74	theme	digestible	2671:2680	arg1	starch					2682:2687	rumen digestible starch	2665:2687	rumen digestible starch concentration	2665:2701	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	2	75	theme	excluding	398:406	arg1	analogs					422:428	excluding nonfunctional analogs	398:428	excluding nonfunctional analogs	398:428	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	2	75	theme	excluding	398:406	arg1	"					362:362	"true"	357:362	"true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply	357:446	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	0	76	theme	synthesis	34:42	arg1	Meta-analysis					0:12	Meta-analysis	0:12	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.	0:92	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	2	77	dep	cannot	453:458	arg1	as					503:504	as	503:504	as	503:504	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	4	78	theme	supplemental	915:926	arg1	vitamins					930:937	supplemental B vitamins	915:937	supplemental B vitamins	915:937	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	11	79	theme	modeling	2294:2301	arg1	ARS					2303:2305	modeling ARS	2294:2305	modeling ARS	2294:2305	Overall, modeling ARS yielded better-performing models compared with modeling PRF, and DMI was included in all prediction equations as a scalar variable.
35879170	13	80	dep	had	2605:2607	arg1	whereas					2657:2663	whereas	2657:2663	whereas	2657:2663	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	0	81	theme	postruminal	48:58	arg1	flow					60:63	postruminal flow	48:63	postruminal flow	48:63	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	4	82	from	absence	904:910	arg1	hypotheses					872:881	hypotheses	872:881	hypotheses of regulation in the absence of supplemental B vitamins	872:937	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	7	83	theme	DM	1639:1640	arg1	g/kg					1631:1634	g/kg	1631:1634	g/kg of DM	1631:1640	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	84	theme	B6	2653:2654	arg1	ARS					2634:2636	the ARS	2630:2636	the ARS of B2, B3, and B6	2630:2654	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	11	85	theme	scalar	2422:2427	arg1	variable					2429:2436	a scalar variable	2420:2436	a scalar variable	2420:2436	Overall, modeling ARS yielded better-performing models compared with modeling PRF, and DMI was included in all prediction equations as a scalar variable.
35879170	8	86	theme	crude	1962:1966	arg1	protein					1968:1974	crude protein	1962:1974	crude protein	1962:1974	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	87	dep	NDF	2000:2002	arg1	g/kg					2005:2008	g/kg	2005:2008	g/kg of DM	2005:2014	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	87	dep	NDF	2000:2002	arg1	forage					1993:1998	forage	1993:1998	forage	1993:1998	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	7	88	theme	neutral	1589:1595	arg1	fiber					1607:1611	rumen digestible neutral detergent fiber	1572:1611	rumen digestible neutral detergent fiber (NDF)	1572:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	88	theme	neutral	1589:1595	arg1	NDF					1614:1616	NDF	1614:1616	NDF	1614:1616	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	5	89	theme	postruminal	1239:1249	arg1	samples					1259:1265	postruminal digesta samples	1239:1265	postruminal digesta samples	1239:1265	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	7	90	theme	VFA	1750:1752	arg1	VFA					1750:1752	VFA	1750:1752	VFA	1750:1752	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	90	theme	VFA	1750:1752	arg1	%					1745:1745	%	1745:1745	% of VFA	1745:1752	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	91	theme	rumen	1572:1576	arg1	fiber					1607:1611	rumen digestible neutral detergent fiber	1572:1611	rumen digestible neutral detergent fiber (NDF)	1572:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	91	theme	rumen	1572:1576	arg1	NDF					1614:1616	NDF	1614:1616	NDF	1614:1616	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	1	92	theme	dairy	222:226	arg1	cow					228:230	the modern dairy cow	211:230	the modern dairy cow	211:230	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	15	93	theme	diet	2961:2964	arg1	effect					2951:2956	the effect	2947:2956	the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow	2947:3038	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	2	94	theme	rumen	510:514	arg1	microbiota					516:525	the rumen microbiota	506:525	the rumen microbiota	506:525	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	13	95	theme	B9	2778:2779	arg1	ARS					2771:2773	the ARS	2767:2773	the ARS of B9	2767:2779	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	14	96	theme	B9	2861:2862	arg1	PRF					2842:2844	PRF	2842:2844	PRF of B1, B2, and B9	2842:2862	In the best prediction models, the dietary starch increased PRF of B1, B2, and B9 but decreased PRF of B12.
35879170	5	97	theme	B	1307:1307	arg1	vitamins					1309:1316	B vitamins	1307:1316	B vitamins (B1, B2, B3, B6, B9, B12)	1307:1342	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	7	98	dep	VFA	1672:1674	arg1	mM					1677:1678	mM	1677:1678	mM	1677:1678	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	99	theme	rumen-level	1542:1552	arg1	fiber					1607:1611	rumen digestible neutral detergent fiber	1572:1611	rumen digestible neutral detergent fiber (NDF)	1572:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	99	theme	rumen-level	1542:1552	arg1	variables					1554:1562	rumen-level variables	1542:1562	rumen-level variables such as rumen digestible neutral detergent fiber (NDF)	1542:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	1	100	theme	suboptimal	269:278	arg1	supply					290:295	suboptimal B-vitamin supply	269:295	suboptimal B-vitamin supply	269:295	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	4	101	theme	regulation	886:895	arg1	hypotheses					872:881	hypotheses	872:881	hypotheses of regulation in the absence of supplemental B vitamins	872:937	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	7	102	theme	matter	1452:1457	arg1	DMI					1467:1469	DMI	1467:1469	DMI	1467:1469	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	102	theme	matter	1452:1457	arg1	intake					1459:1464	dry matter intake	1448:1464	dry matter intake (DMI, kg/d)	1448:1476	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	4	103	theme	true	1006:1009	arg1	supply					1021:1026	true B-vitamin supply	1006:1026	true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS)	1006:1098	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	7	104	theme	%	1820:1820	arg1	/h					1821:1822	%/h	1820:1822	%/h	1820:1822	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	104	theme	%	1820:1820	arg1	NDF					1804:1806	NDF	1804:1806	NDF	1804:1806	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	3	105	theme	cows	667:670	arg1	metabolism					647:656	the metabolism	643:656	the metabolism of dairy cows	643:670	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	12	106	theme	B6	2527:2528	arg1	ARS					2504:2506	the ARS	2500:2506	the ARS of B1, B2, B3, and B6	2500:2528	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	8	107	theme	driving	1873:1879	arg1	DMI					1899:1901	DMI	1899:1901	DMI	1899:1901	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	107	theme	driving	1873:1879	arg1	starch					1951:1956	starch	1951:1956	starch	1951:1956	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	107	theme	driving	1873:1879	arg1	NDF					2000:2002	forage NDF	1993:2002	forage NDF (g/kg of DM)	1993:2015	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	107	theme	driving	1873:1879	arg1	concentration					1922:1934	B-vitamin dietary concentration	1904:1934	B-vitamin dietary concentration (mg/kg of DM)	1904:1948	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	8	107	theme	driving	1873:1879	arg1	variables					1881:1889	dietary-level driving variables	1859:1889	dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM)	1859:2015	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	7	108	theme	starch	1812:1817	arg1	degradation					1789:1799	degradation	1789:1799	degradation of NDF and starch (%/h)	1789:1823	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	12	109	theme	B2	2515:2516	arg1	ARS					2504:2506	the ARS	2500:2506	the ARS of B1, B2, B3, and B6	2500:2528	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	5	110	with	studies	1195:1201	arg1	experiments					1211:1221	16 experiments	1208:1221	16 experiments	1208:1221	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	7	111	theme	describing	1422:1431	arg1	ARS					1433:1435	Models describing ARS	1415:1435	Models describing ARS	1415:1435	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	112	contain	had	2703:2705	arg2	effect					2718:2723	a negative effect	2707:2723	a negative effect on the ARS of B1	2707:2740	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	13	112	contain	had	2703:2705	arg2	effect					2757:2762	a positive effect	2746:2762	a positive effect on the ARS of B9	2746:2779	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	13	112	contain	had	2703:2705	arg1	concentration					2689:2701	rumen digestible starch concentration	2665:2701	rumen digestible starch concentration	2665:2701	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	14	113	theme	B2	2853:2854	arg1	PRF					2842:2844	PRF	2842:2844	PRF of B1, B2, and B9	2842:2862	In the best prediction models, the dietary starch increased PRF of B1, B2, and B9 but decreased PRF of B12.
35879170	5	114	from	studies	1195:1201	arg1	observations					1174:1185	340 individual cow observations	1155:1185	340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12)	1155:1342	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	114	from	studies	1195:1201	arg1	hoc					1278:1280	post hoc	1273:1280	post hoc	1273:1280	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	7	115	theme	NDF	1804:1806	arg1	degradation					1789:1799	degradation	1789:1799	degradation of NDF and starch (%/h)	1789:1823	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	3	116	theme	key	693:695	arg1	nutrients					697:705	these key nutrients	687:705	these key nutrients	687:705	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	1	117	theme	milk	97:100	arg1	production					102:111	milk production	97:111	milk production	97:111	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	15	118	theme	true	2997:3000	arg1	supply					3002:3007	the true supply	2993:3007	the true supply of B vitamins to the dairy cow	2993:3038	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	13	119	theme	negative	2611:2618	arg1	effect					2620:2625	a negative effect	2609:2625	a negative effect	2609:2625	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	14	120	theme	B1	2849:2850	arg1	PRF					2842:2844	PRF	2842:2844	PRF of B1, B2, and B9	2842:2862	In the best prediction models, the dietary starch increased PRF of B1, B2, and B9 but decreased PRF of B12.
35879170	13	121	theme	positive	2748:2755	arg1	effect					2757:2762	a positive effect	2746:2762	a positive effect on the ARS of B9	2746:2779	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	4	122	theme	ruminal	1076:1082	arg1	ARS					1095:1097	ARS	1095:1097	ARS	1095:1097	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	4	122	theme	ruminal	1076:1082	arg1	synthesis					1084:1092	apparent ruminal synthesis	1067:1092	apparent ruminal synthesis (ARS)	1067:1098	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	7	123	theme	degradation	1789:1799	arg1	pH					1761:1762	mean pH	1756:1762	mean pH	1756:1762	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	butyrate					1703:1710	butyrate	1703:1710	butyrate	1703:1710	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	starch					1623:1628	starch	1623:1628	starch	1623:1628	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	acids					1665:1669	total volatile fatty acids	1644:1669	total volatile fatty acids (VFA, mM)	1644:1679	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	valerate					1717:1724	valerate	1717:1724	valerate	1717:1724	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	acetate					1682:1688	acetate	1682:1688	acetate	1682:1688	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	[mg/kg					1511:1516	B-vitamin dietary concentration [mg/kg	1479:1516	B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF)	1479:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	intake					1459:1464	dry matter intake	1448:1464	dry matter intake (DMI, kg/d)	1448:1476	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	propionate					1691:1700	propionate	1691:1700	propionate	1691:1700	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	rates					1780:1784	fractional rates	1769:1784	fractional rates of degradation of NDF and starch (%/h)	1769:1823	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	VFA					1672:1674	VFA	1672:1674	VFA	1672:1674	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	123	theme	degradation	1789:1799	arg1	DMI					1467:1469	DMI	1467:1469	DMI	1467:1469	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	12	124	theme	B9	2562:2563	arg1	PRF					2548:2550	the PRF	2544:2550	the PRF of B2 and B9	2544:2563	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	6	125	theme	linear	1386:1391	arg1	form					1393:1396	univariate and multivariate linear form	1358:1396	univariate and multivariate linear form	1358:1396	Equations of univariate and multivariate linear form were considered.
35879170	13	126	theme	rumen	2570:2574	arg1	concentration					2591:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	0	127	from	cows	88:91	arg1	Meta-analysis					0:12	Meta-analysis	0:12	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.	0:92	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	8	128	theme	B-vitamin	1904:1912	arg1	concentration					1922:1934	B-vitamin dietary concentration	1904:1934	B-vitamin dietary concentration (mg/kg of DM)	1904:1948	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	5	129	theme	individual	1159:1168	arg1	observations					1174:1185	340 individual cow observations	1155:1185	340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12)	1155:1342	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	5	129	theme	individual	1159:1168	arg1	hoc					1278:1280	post hoc	1273:1280	post hoc	1273:1280	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	3	130	theme	next	716:719	arg1	generation					721:730	the next generation	712:730	the next generation of mathematical models	712:753	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	0	131	theme	B	68:68	arg1	vitamins					70:77	B vitamins	68:77	B vitamins	68:77	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	9	132	theme	significant	2067:2077	arg1	parameters					2085:2094	all significant slope parameters	2063:2094	all significant slope parameters	2063:2094	Equations developed were required to contain all significant slope parameters and contained no significant collinearity between driving variables.
35879170	4	133	dep	cow	1035:1037	arg1	PRF					1058:1060	PRF	1058:1060	PRF	1058:1060	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	4	133	dep	cow	1035:1037	arg1	flow					1052:1055	postruminal flow	1040:1055	postruminal flow	1040:1055	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	13	134	theme	NDF	2587:2589	arg1	concentration					2591:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration	2566:2603	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	12	135	theme	negative	2481:2488	arg1	effect					2490:2495	a negative effect	2479:2495	a negative effect	2479:2495	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	15	136	theme	vitamins	3014:3021	arg1	supply					3002:3007	the true supply	2993:3007	the true supply of B vitamins to the dairy cow	2993:3038	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	12	137	theme	dietary	2453:2459	arg1	concentration					2461:2473	The B-vitamin dietary concentration	2439:2473	The B-vitamin dietary concentration	2439:2473	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	2	138	dep	forms	391:395	arg1	i.e.					365:368	i.e.	365:368	i.e.	365:368	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	7	139	theme	dry	1521:1523	arg1	DM					1533:1534	DM	1533:1534	DM	1533:1534	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	139	theme	dry	1521:1523	arg1	matter					1525:1530	dry matter	1521:1530	dry matter (DM)]	1521:1536	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	2	140	theme	nonfunctional	408:420	arg1	analogs					422:428	excluding nonfunctional analogs	398:428	excluding nonfunctional analogs	398:428	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	2	140	theme	nonfunctional	408:420	arg1	"					362:362	"true"	357:362	"true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply	357:446	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	3	141	theme	models	748:753	arg1	generation					721:730	the next generation	712:730	the next generation of mathematical models	712:753	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	1	142	theme	metabolic	308:316	arg1	efficiency					318:327	metabolic efficiency	308:327	metabolic efficiency	308:327	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	15	143	used	used	2921:2924	arg2	equations					2894:2902	The equations	2890:2902	The equations developed	2890:2912	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	3	144	theme	animal	784:789	arg1	production					791:800	animal production	784:800	animal production	784:800	Given their complex impact on the metabolism of dairy cows, incorporating these key nutrients into the next generation of mathematical models could help to better predict animal production and performance.
35879170	4	145	theme	study	850:854	arg1	purpose					834:840	the purpose	830:840	the purpose of this study	830:854	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	0	146	from	flow	60:63	arg1	cows					88:91	dairy cows	82:91	dairy cows	82:91	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	9	147	theme	significant	2113:2123	arg1	collinearity					2125:2136	no significant collinearity	2110:2136	no significant collinearity between driving variables	2110:2162	Equations developed were required to contain all significant slope parameters and contained no significant collinearity between driving variables.
35879170	11	148	theme	prediction	2396:2405	arg1	equations					2407:2415	all prediction equations	2392:2415	all prediction equations as a scalar variable	2392:2436	Overall, modeling ARS yielded better-performing models compared with modeling PRF, and DMI was included in all prediction equations as a scalar variable.
35879170	7	149	theme	dietary	1489:1495	arg1	[mg/kg					1511:1516	B-vitamin dietary concentration [mg/kg	1479:1516	B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF)	1479:1617	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	14	150	theme	best	2789:2792	arg1	models					2805:2810	the best prediction models	2785:2810	the best prediction models	2785:2810	In the best prediction models, the dietary starch increased PRF of B1, B2, and B9 but decreased PRF of B12.
35879170	2	151	theme	B	601:601	arg1	vitamins					603:610	B vitamins	601:610	B vitamins	601:610	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	1	152	theme	B-vitamin	192:200	arg1	estimates					175:183	existing estimates	166:183	existing estimates of the B-vitamin needs of the modern dairy cow	166:230	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	7	153	theme	fatty	1659:1663	arg1	VFA					1672:1674	VFA	1672:1674	VFA	1672:1674	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	153	theme	fatty	1659:1663	arg1	acids					1665:1669	total volatile fatty acids	1644:1669	total volatile fatty acids (VFA, mM)	1644:1679	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	154	theme	negative	2709:2716	arg1	effect					2718:2723	a negative effect	2707:2723	a negative effect on the ARS of B1	2707:2740	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	4	155	theme	B	928:928	arg1	vitamins					930:937	supplemental B vitamins	915:937	supplemental B vitamins	915:937	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	2	156	theme	same	337:340	arg1	time					342:345	the same time	333:345	the same time	333:345	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	0	157	theme	ruminal	26:32	arg1	synthesis					34:42	apparent ruminal synthesis	17:42	apparent ruminal synthesis	17:42	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	7	158	theme	total	1644:1648	arg1	VFA					1672:1674	VFA	1672:1674	VFA	1672:1674	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	7	158	theme	total	1644:1648	arg1	acids					1665:1669	total volatile fatty acids	1644:1669	total volatile fatty acids (VFA, mM)	1644:1679	Models describing ARS considered dry matter intake (DMI, kg/d), B-vitamin dietary concentration [mg/kg of dry matter (DM)] and rumen-level variables such as rumen digestible neutral detergent fiber (NDF) and starch (g/kg of DM), total volatile fatty acids (VFA, mM), acetate, propionate, butyrate, and valerate molar proportions (% of VFA), mean pH, and fractional rates of degradation of NDF and starch (%/h).
35879170	13	159	theme	rumen	2665:2669	arg1	starch					2682:2687	rumen digestible starch	2665:2687	rumen digestible starch concentration	2665:2701	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	2	160	theme	true	358:361	arg1	analogs					422:428	excluding nonfunctional analogs	398:428	excluding nonfunctional analogs	398:428	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	2	160	theme	true	358:361	arg1	"					362:362	"true"	357:362	"true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply	357:446	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	15	161	from	effect	2951:2956	arg1	supply					3002:3007	the true supply	2993:3007	the true supply of B vitamins to the dairy cow	2993:3038	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	13	162	theme	starch	2682:2687	arg1	concentration					2689:2701	rumen digestible starch concentration	2665:2701	rumen digestible starch concentration	2665:2701	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	10	163	theme	data	2270:2273	arg1	scarcity					2275:2282	data scarcity	2270:2282	data scarcity	2270:2282	Concordance correlation coefficient was used to evaluate the models on the developmental data set due to data scarcity.
35879170	5	164	theme	digesta	1251:1257	arg1	samples					1259:1265	postruminal digesta samples	1239:1265	postruminal digesta samples	1239:1265	The database used for this meta-analysis consisted of 340 individual cow observations from 15 studies with 16 experiments, where diet and postruminal digesta samples were (post hoc) analyzed for content of B vitamins (B1, B2, B3, B6, B9, B12).
35879170	0	165	theme	flow	60:63	arg1	Meta-analysis					0:12	Meta-analysis	0:12	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.	0:92	Meta-analysis of apparent ruminal synthesis and postruminal flow of B vitamins in dairy cows.
35879170	8	166	theme	DM	1946:1947	arg1	mg/kg					1937:1941	mg/kg	1937:1941	mg/kg of DM	1937:1947	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	2	167	theme	active	384:389	arg1	forms					391:395	biologically active forms	371:395	biologically active forms	371:395	At the same time, however, "true" (i.e., biologically active forms, excluding nonfunctional analogs) B-vitamin supply also cannot be adequately estimated by dietary intake, as the rumen microbiota has been shown to play a significant role in synthesis and utilization of B vitamins.
35879170	8	168	dep	concentration	1922:1934	arg1	mg/kg					1937:1941	mg/kg	1937:1941	mg/kg of DM	1937:1947	Models describing PRF considered dietary-level driving variables such as DMI, B-vitamin dietary concentration (mg/kg of DM), starch and crude protein (g/kg of DM) and forage NDF (g/kg of DM).
35879170	1	169	theme	B-vitamin	280:288	arg1	supply					290:295	suboptimal B-vitamin supply	269:295	suboptimal B-vitamin supply	269:295	As milk production has significantly increased over the past decade(s), existing estimates of the B-vitamin needs of the modern dairy cow are currently being reconsidered, as suboptimal B-vitamin supply may affect metabolic efficiency.
35879170	15	170	theme	requirements	3088:3099	arg1	development					3058:3068	the development	3054:3068	the development of better-defined requirements in the future	3054:3113	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	12	171	theme	B3	2519:2520	arg1	ARS					2504:2506	the ARS	2500:2506	the ARS of B1, B2, B3, and B6	2500:2528	The B-vitamin dietary concentration had a negative effect on the ARS of B1, B2, B3, and B6 but increased the PRF of B2 and B9.
35879170	13	172	from	effect	2757:2762	arg1	ARS					2732:2734	the ARS	2728:2734	the ARS of B1	2728:2740	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	13	172	from	effect	2757:2762	arg1	ARS					2771:2773	the ARS	2767:2773	the ARS of B9	2767:2779	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	4	173	from	regulation	886:895	arg1	absence					904:910	the absence	900:910	the absence of supplemental B vitamins	900:937	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
35879170	15	174	theme	environment	2978:2988	arg1	effect					2951:2956	the effect	2947:2956	the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow	2947:3038	The equations developed may be used to better understand the effect of diet and ruminal environment on the true supply of B vitamins to the dairy cow and stimulate the development of better-defined requirements in the future.
35879170	13	175	theme	B2	2641:2642	arg1	ARS					2634:2636	the ARS	2630:2636	the ARS of B2, B3, and B6	2630:2654	The rumen digestible NDF concentration had a negative effect on the ARS of B2, B3, and B6, whereas rumen digestible starch concentration had a negative effect on the ARS of B1 and a positive effect on the ARS of B9.
35879170	10	176	from	models	2226:2231	arg1	data					2254:2257	the developmental data	2236:2257	the developmental data set due to data scarcity	2236:2282	Concordance correlation coefficient was used to evaluate the models on the developmental data set due to data scarcity.
35879170	14	177	theme	B12	2885:2887	arg1	PRF					2878:2880	PRF	2878:2880	PRF of B12	2878:2887	In the best prediction models, the dietary starch increased PRF of B1, B2, and B9 but decreased PRF of B12.
35879170	4	178	from	hypotheses	872:881	arg1	absence					904:910	the absence	900:910	the absence of supplemental B vitamins	900:937	Therefore, the purpose of this study was to generate hypotheses of regulation in the absence of supplemental B vitamins by creating empirical models, through a meta-analysis, to describe true B-vitamin supply to the cow (postruminal flow, PRF) and apparent ruminal synthesis (ARS).
36534956	2	0	theme	experiment	514:523	arg1	beginning					497:505	the beginning	493:505	the beginning of the experiment	493:523	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	8	1	theme	relative	1481:1488	arg1	abundance					1490:1498	the relative abundance	1477:1498	the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level	1477:1558	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	0	2	theme	dairy	164:168	arg1	cows					170:173	dairy cows	164:173	dairy cows	164:173	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	12	3	theme	cows	2304:2307	arg1	rumen					2277:2281	rumen	2277:2281	rumen	2277:2281	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	12	3	theme	cows	2304:2307	arg1	hindgut					2287:2293	hindgut	2287:2293	hindgut	2287:2293	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	6	4	theme	P	1154:1154	arg1	0.015					1158:1162	P = 0.015	1154:1162	P = 0.015	1154:1162	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	4	theme	P	1154:1154	arg1	yield					1147:1151	milk yield	1142:1151	milk yield (P = 0.015)	1142:1163	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	11	5	theme	LY	2015:2016	arg1	supplementation					2018:2032	LY supplementation	2015:2032	LY supplementation	2015:2032	In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows.
36534956	8	6	theme	Miseq	1413:1417	arg1	sequencing					1419:1428	Miseq sequencing	1413:1428	Miseq sequencing of the 16S rRNA genes	1413:1450	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	6	7	theme	milk	1198:1201	arg1	P					1218:1218	P = 0.008	1218:1226	P = 0.008	1218:1226	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	7	theme	milk	1198:1201	arg1	yield					1211:1215	milk protein yield	1198:1215	milk protein yield (P = 0.008)	1198:1227	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	7	theme	milk	1198:1201	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	5	8	theme	milk	978:981	arg1	0.035					1016:1020	P = 0.035	1012:1020	P = 0.035	1012:1020	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	8	theme	milk	978:981	arg1	concentration					997:1009	decreased milk urea nitrogen concentration	968:1009	decreased milk urea nitrogen concentration (P = 0.035)	968:1021	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	10	9	theme	=	1774:1774	arg1	0.011					1776:1780	P = 0.011	1772:1780	P = 0.011	1772:1780	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	9	theme	=	1774:1774	arg1	Actinobacillus					1756:1769	Actinobacillus	1756:1769	Actinobacillus (P = 0.011)	1756:1781	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	7	10	theme	acids	1377:1381	arg1	concentrations					1293:1306	the concentrations	1289:1306	the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05)	1289:1410	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	11	11	theme	heat	2172:2175	arg1	stress					2177:2182	heat stress	2172:2182	heat stress	2172:2182	In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows.
36534956	0	12	theme	Live	0:3	arg1	supplementation					11:25	Live yeast supplementation	0:25	Live yeast supplementation	0:25	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	7	13	theme	volatile	1362:1369	arg1	acids					1377:1381	total volatile fatty acids	1356:1381	total volatile fatty acids (VFAs)	1356:1388	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	7	13	theme	volatile	1362:1369	arg1	VFAs					1384:1387	VFAs	1384:1387	VFAs	1384:1387	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	8	14	theme	16S	1437:1439	arg1	genes					1446:1450	the 16S rRNA genes	1433:1450	the 16S rRNA genes	1433:1450	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	6	15	theme	=	1188:1188	arg1	P					1186:1186	P = 0.001	1186:1194	P = 0.001	1186:1194	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	15	theme	=	1188:1188	arg1	nitrogen					1176:1183	milk urea nitrogen	1166:1183	milk urea nitrogen (P = 0.001)	1166:1195	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	10	16	theme	pathways	1932:1939	arg1	series					1913:1918	a series	1911:1918	a series of enriched pathways in carbohydrate metabolism	1911:1966	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	6	17	theme	rectal	1082:1087	arg1	<					1133:1133	P < 0.05	1131:1138	P < 0.05	1131:1138	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	17	theme	rectal	1082:1087	arg1	yield					1247:1251	milk lactose yield	1234:1251	milk lactose yield	1234:1251	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	17	theme	rectal	1082:1087	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	17	theme	rectal	1082:1087	arg1	nitrogen					1176:1183	milk urea nitrogen	1166:1183	milk urea nitrogen (P = 0.001)	1166:1195	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	17	theme	rectal	1082:1087	arg1	rate					1125:1128	respiratory rate	1113:1128	respiratory rate	1113:1128	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	17	theme	rectal	1082:1087	arg1	yield					1211:1215	milk protein yield	1198:1215	milk protein yield (P = 0.008)	1198:1227	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	17	theme	rectal	1082:1087	arg1	yield					1147:1151	milk yield	1142:1151	milk yield (P = 0.015)	1142:1163	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	17	theme	rectal	1082:1087	arg1	<					1104:1104	P < 0.05	1102:1109	P < 0.05	1102:1109	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	5	18	theme	milk	880:883	arg1	yield					885:889	milk yield	880:889	milk yield	880:889	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	18	theme	milk	880:883	arg1	P					952:952	P < 0.001	952:960	P < 0.001	952:960	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	2	19	theme	±	474:474	arg1	d					480:480	189.1 ± 6.6 d	468:480	189.1 ± 6.6 d in milk at the beginning of the experiment	468:523	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	0	20	from	function	77:84	arg1	hindgut					99:105	hindgut	99:105	hindgut	99:105	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	0	20	from	function	77:84	arg1	rumen					89:93	rumen	89:93	rumen	89:93	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	7	21	theme	isovaterate	1333:1343	arg1	concentrations					1293:1306	the concentrations	1289:1306	the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05)	1289:1410	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	0	22	theme	detrimental	126:136	arg1	effects					138:144	the detrimental effects	122:144	the detrimental effects of heat stress on dairy cows	122:173	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	12	23	dep	rumen	2277:2281	arg1	the					2273:2275	the	2273:2275	the	2273:2275	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	1	24	theme	study	198:202	arg1	objective					180:188	The objective	176:188	The objective of this study	176:202	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	4	25	theme	yeast	724:728	arg1	product					730:736	The yeast product	720:736	The yeast product	720:736	The yeast product contained 2.0 × 10 10 CFU/g.
36534956	0	26	from	composition	61:71	arg1	hindgut					99:105	hindgut	99:105	hindgut	99:105	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	0	26	from	composition	61:71	arg1	rumen					89:93	rumen	89:93	rumen	89:93	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	6	27	theme	milk	1142:1145	arg1	0.015					1158:1162	P = 0.015	1154:1162	P = 0.015	1154:1162	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	27	theme	milk	1142:1145	arg1	0.030					1258:1262	P = 0.030	1254:1262	P = 0.030	1254:1262	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	27	theme	milk	1142:1145	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	27	theme	milk	1142:1145	arg1	yield					1147:1151	milk yield	1142:1151	milk yield (P = 0.015)	1142:1163	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	28	theme	week	1059:1062	arg1	effects					1036:1042	Interaction effects	1024:1042	Interaction effects of treatment × week	1024:1062	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	5	29	theme	dry	861:863	arg1	intake					872:877	dry matter intake	861:877	dry matter intake	861:877	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	3	30	theme	20 g	693:696	arg1	LY-20					712:716	LY-20	712:716	LY-20	712:716	Cows in the three groups were fed a diet without yeast (CON), with 10 g yeast/d/head (LY-10), and with 20 g yeast/d/head (LY-20).
36534956	3	30	theme	20 g	693:696	arg1	yeast/d/head					698:709	20 g yeast/d/head	693:709	20 g yeast/d/head (LY-20)	693:717	Cows in the three groups were fed a diet without yeast (CON), with 10 g yeast/d/head (LY-10), and with 20 g yeast/d/head (LY-20).
36534956	10	31	theme	relative	1800:1807	arg1	abundance					1809:1817	the relative abundance	1796:1817	the relative abundance by LY	1796:1823	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	1	32	dep	yeast	243:247	arg1	LY					250:251	LY	250:251	LY	250:251	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	32	dep	yeast	243:247	arg1	cerevisiae					268:277	Saccharomyces cerevisiae	254:277	Saccharomyces cerevisiae	254:277	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	10	33	theme	carbohydrate	1944:1955	arg1	metabolism					1957:1966	carbohydrate metabolism	1944:1966	carbohydrate metabolism	1944:1966	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	3	34	theme	10 g	657:660	arg1	LY-10					676:680	LY-10	676:680	LY-10	676:680	Cows in the three groups were fed a diet without yeast (CON), with 10 g yeast/d/head (LY-10), and with 20 g yeast/d/head (LY-20).
36534956	3	34	theme	10 g	657:660	arg1	yeast/d/head					662:673	10 g yeast/d/head	657:673	10 g yeast/d/head (LY-10)	657:681	Cows in the three groups were fed a diet without yeast (CON), with 10 g yeast/d/head (LY-10), and with 20 g yeast/d/head (LY-20).
36534956	6	35	theme	treatment	1047:1055	arg1	week					1059:1062	treatment × week	1047:1062	treatment × week	1047:1062	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	1	36	theme	dairy	373:377	arg1	cows					379:382	dairy cows	373:382	dairy cows	373:382	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	7	37	theme	isobutyrate	1320:1330	arg1	concentrations					1293:1306	the concentrations	1289:1306	the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05)	1289:1410	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	10	38	from	series	1913:1918	arg1	metabolism					1957:1966	carbohydrate metabolism	1944:1966	carbohydrate metabolism	1944:1966	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	5	39	theme	decreased	968:976	arg1	0.035					1016:1020	P = 0.035	1012:1020	P = 0.035	1012:1020	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	39	theme	decreased	968:976	arg1	concentration					997:1009	decreased milk urea nitrogen concentration	968:1009	decreased milk urea nitrogen concentration (P = 0.035)	968:1021	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	40	theme	protein	913:919	arg1	yield					921:925	milk protein yield	908:925	milk protein yield	908:925	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	7	41	theme	acetate	1311:1317	arg1	concentrations					1293:1306	the concentrations	1289:1306	the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05)	1289:1410	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	0	42	theme	stress	154:159	arg1	effects					138:144	the detrimental effects	122:144	the detrimental effects of heat stress on dairy cows	122:173	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	10	43	theme	bile	1979:1982	arg1	biosynthesis					1989:2000	secondary bile acid biosynthesis	1969:2000	secondary bile acid biosynthesis	1969:2000	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	5	44	theme	=	1014:1014	arg1	0.035					1016:1020	P = 0.035	1012:1020	P = 0.035	1012:1020	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	44	theme	=	1014:1014	arg1	concentration					997:1009	decreased milk urea nitrogen concentration	968:1009	decreased milk urea nitrogen concentration (P = 0.035)	968:1021	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	12	45	theme	live	2259:2262	arg1	yeast					2264:2268	live yeast	2259:2268	live yeast	2259:2268	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	8	46	from	level	1554:1558	arg1	abundance					1490:1498	the relative abundance	1477:1498	the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level	1477:1558	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	10	47	theme	Clostridium	1712:1722	arg1	0.013					1745:1749	P = 0.013	1741:1749	P = 0.013	1741:1749	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	47	theme	Clostridium	1712:1722	arg1	stricto					1730:1736	Clostridium sensu stricto 1	1712:1738	Clostridium sensu stricto 1 (P = 0.013)	1712:1750	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	5	48	theme	fat	897:899	arg1	yield					901:905	milk fat yield	892:905	milk fat yield	892:905	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	6	49	theme	P	1254:1254	arg1	yield					1147:1151	milk yield	1142:1151	milk yield (P = 0.015)	1142:1163	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	49	theme	P	1254:1254	arg1	0.030					1258:1262	P = 0.030	1254:1262	P = 0.030	1254:1262	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	2	50	theme	dairy	456:460	arg1	cows					462:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows	403:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment)	403:524	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	8	51	from	pathways	1586:1593	arg1	metabolism					1602:1611	the metabolism	1598:1611	the metabolism of carbohydrates, and protein	1598:1641	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	10	52	from	metabolism	1957:1966	arg1	series					1913:1918	a series	1911:1918	a series of enriched pathways in carbohydrate metabolism	1911:1966	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	1	53	theme	yeast	243:247	arg1	effects					227:233	the effects	223:233	the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress	223:400	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	8	54	theme	enriched	1577:1584	arg1	pathways					1586:1593	enriched pathways	1577:1593	enriched pathways in the metabolism of carbohydrates, and protein	1577:1641	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	1	55	from	functions	335:343	arg1	rumen					352:356	rumen	352:356	rumen	352:356	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	55	from	functions	335:343	arg1	hindgut					362:368	hindgut	362:368	hindgut	362:368	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	6	56	theme	milk	1234:1237	arg1	yield					1247:1251	milk lactose yield	1234:1251	milk lactose yield	1234:1251	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	56	theme	milk	1234:1237	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	2	57	from	beginning	497:505	arg1	d					480:480	189.1 ± 6.6 d	468:480	189.1 ± 6.6 d in milk at the beginning of the experiment	468:523	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	0	58	dep	composition	61:71	arg1	the					35:37	the	35:37	the	35:37	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	1	59	dep	performance	297:307	arg1	the					283:285	the	283:285	the	283:285	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	5	60	theme	milk	932:935	arg1	yield					945:949	milk lactose yield	932:949	milk lactose yield	932:949	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	2	61	dep	cows	462:465	arg1	d					480:480	189.1 ± 6.6 d	468:480	189.1 ± 6.6 d in milk at the beginning of the experiment	468:523	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	8	62	theme	protein	1635:1641	arg1	metabolism					1602:1611	the metabolism	1598:1611	the metabolism of carbohydrates, and protein	1598:1641	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	2	63	theme	multiparous	416:426	arg1	cows					462:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows	403:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment)	403:524	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	6	64	theme	respiratory	1113:1123	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	64	theme	respiratory	1113:1123	arg1	rate					1125:1128	respiratory rate	1113:1128	respiratory rate	1113:1128	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	12	65	theme	heat	2320:2323	arg1	stress					2325:2330	heat stress	2320:2330	heat stress	2320:2330	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	1	66	from	performance	297:307	arg1	rumen					352:356	rumen	352:356	rumen	352:356	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	66	from	performance	297:307	arg1	hindgut					362:368	hindgut	362:368	hindgut	362:368	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	67	theme	lactation	287:295	arg1	performance					297:307	lactation performance	287:307	lactation performance	287:307	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	8	68	from	metabolism	1602:1611	arg1	series					1567:1572	a series	1565:1572	a series of enriched pathways in the metabolism of carbohydrates, and protein	1565:1641	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	6	69	theme	milk	1166:1169	arg1	P					1186:1186	P = 0.001	1186:1194	P = 0.001	1186:1194	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	69	theme	milk	1166:1169	arg1	nitrogen					1176:1183	milk urea nitrogen	1166:1183	milk urea nitrogen (P = 0.001)	1166:1195	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	69	theme	milk	1166:1169	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	5	70	theme	urea	983:986	arg1	0.035					1016:1020	P = 0.035	1012:1020	P = 0.035	1012:1020	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	70	theme	urea	983:986	arg1	concentration					997:1009	decreased milk urea nitrogen concentration	968:1009	decreased milk urea nitrogen concentration (P = 0.035)	968:1021	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	71	theme	respiratory	821:831	arg1	rate					833:836	respiratory rate	821:836	respiratory rate of cows	821:844	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	11	72	from	effects	2161:2167	arg1	cows					2193:2196	dairy cows	2187:2196	dairy cows	2187:2196	In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows.
36534956	10	73	theme	acid	1984:1987	arg1	biosynthesis					1989:2000	secondary bile acid biosynthesis	1969:2000	secondary bile acid biosynthesis	1969:2000	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	12	74	theme	dairy	2298:2302	arg1	cows					2304:2307	dairy cows	2298:2307	dairy cows exposed to heat stress	2298:2330	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	12	75	theme	extended	2222:2229	arg1	insight					2231:2237	extended insight	2222:2237	extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress	2222:2330	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	9	76	theme	fecal	1647:1651	arg1	samples					1653:1659	fecal samples	1647:1659	fecal samples	1647:1659	In fecal samples, LY did not affect the profile of VFAs (P > 0.05).
36534956	6	77	theme	=	1156:1156	arg1	0.015					1158:1162	P = 0.015	1154:1162	P = 0.015	1154:1162	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	77	theme	=	1156:1156	arg1	yield					1147:1151	milk yield	1142:1151	milk yield (P = 0.015)	1142:1163	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	11	78	theme	detrimental	2149:2159	arg1	effects					2161:2167	the detrimental effects	2145:2167	the detrimental effects of heat stress on dairy cows	2145:2196	In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows.
36534956	6	79	theme	Interaction	1024:1034	arg1	effects					1036:1042	Interaction effects	1024:1042	Interaction effects of treatment × week	1024:1062	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	1	80	theme	heat	390:393	arg1	stress					395:400	heat stress	390:400	heat stress	390:400	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	81	theme	live	238:241	arg1	yeast					243:247	live yeast	238:247	live yeast (LY, Saccharomyces cerevisiae)	238:278	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	10	82	theme	=	1743:1743	arg1	0.013					1745:1749	P = 0.013	1741:1749	P = 0.013	1741:1749	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	82	theme	=	1743:1743	arg1	stricto					1730:1736	Clostridium sensu stricto 1	1712:1738	Clostridium sensu stricto 1 (P = 0.013)	1712:1750	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	7	83	theme	fatty	1371:1375	arg1	acids					1377:1381	total volatile fatty acids	1356:1381	total volatile fatty acids (VFAs)	1356:1388	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	7	83	theme	fatty	1371:1375	arg1	VFAs					1384:1387	VFAs	1384:1387	VFAs	1384:1387	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	8	84	theme	pathways	1586:1593	arg1	series					1567:1572	a series	1565:1572	a series of enriched pathways in the metabolism of carbohydrates, and protein	1565:1641	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	10	85	theme	P	1772:1772	arg1	0.011					1776:1780	P = 0.011	1772:1780	P = 0.011	1772:1780	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	85	theme	P	1772:1772	arg1	Actinobacillus					1756:1769	Actinobacillus	1756:1769	Actinobacillus (P = 0.011)	1756:1781	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	7	86	theme	total	1356:1360	arg1	acids					1377:1381	total volatile fatty acids	1356:1381	total volatile fatty acids (VFAs)	1356:1388	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	7	86	theme	total	1356:1360	arg1	VFAs					1384:1387	VFAs	1384:1387	VFAs	1384:1387	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	11	87	theme	stress	2177:2182	arg1	effects					2161:2167	the detrimental effects	2145:2167	the detrimental effects of heat stress on dairy cows	2145:2196	In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows.
36534956	8	88	theme	rRNA	1441:1444	arg1	genes					1446:1450	the 16S rRNA genes	1433:1450	the 16S rRNA genes	1433:1450	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	7	89	theme	valerate	1346:1353	arg1	concentrations					1293:1306	the concentrations	1289:1306	the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05)	1289:1410	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	10	90	theme	enriched	1923:1930	arg1	pathways					1932:1939	enriched pathways	1923:1939	enriched pathways in carbohydrate metabolism	1923:1966	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	9	91	theme	VFAs	1695:1698	arg1	profile					1684:1690	the profile	1680:1690	the profile of VFAs (P > 0.05)	1680:1709	In fecal samples, LY did not affect the profile of VFAs (P > 0.05).
36534956	1	92	from	effects	227:233	arg1	performance					297:307	lactation performance	287:307	lactation performance	287:307	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	92	from	effects	227:233	arg1	community					320:328	bacterial community	310:328	bacterial community	310:328	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	92	from	effects	227:233	arg1	functions					335:343	functions	335:343	functions	335:343	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	2	93	theme	189.1	468:472	arg1	d					480:480	189.1 ± 6.6 d	468:480	189.1 ± 6.6 d in milk at the beginning of the experiment	468:523	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	11	94	theme	dairy	2187:2191	arg1	cows					2193:2196	dairy cows	2187:2196	dairy cows	2187:2196	In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows.
36534956	3	95	from	Cows	590:593	arg1	groups					608:613	the three groups	598:613	the three groups	598:613	Cows in the three groups were fed a diet without yeast (CON), with 10 g yeast/d/head (LY-10), and with 20 g yeast/d/head (LY-20).
36534956	9	96	theme	P	1701:1701	arg1	VFAs					1695:1698	VFAs	1695:1698	VFAs (P > 0.05)	1695:1709	In fecal samples, LY did not affect the profile of VFAs (P > 0.05).
36534956	9	96	theme	P	1701:1701	arg1	>					1703:1703	P > 0.05	1701:1708	P > 0.05	1701:1708	In fecal samples, LY did not affect the profile of VFAs (P > 0.05).
36534956	0	97	theme	heat	149:152	arg1	stress					154:159	heat stress	149:159	heat stress	149:159	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	5	98	theme	milk	892:895	arg1	yield					901:905	milk fat yield	892:905	milk fat yield	892:905	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	6	99	theme	×	1057:1057	arg1	week					1059:1062	treatment × week	1047:1062	treatment × week	1047:1062	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	5	100	theme	matter	865:870	arg1	intake					872:877	dry matter intake	861:877	dry matter intake	861:877	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	6	101	theme	=	1220:1220	arg1	P					1218:1218	P = 0.008	1218:1226	P = 0.008	1218:1226	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	101	theme	=	1220:1220	arg1	yield					1211:1215	milk protein yield	1198:1215	milk protein yield (P = 0.008)	1198:1227	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	8	102	theme	Prevotella	1503:1512	arg1	abundance					1490:1498	the relative abundance	1477:1498	the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level	1477:1558	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	1	103	theme	cows	379:382	arg1	rumen					352:356	rumen	352:356	rumen	352:356	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	103	theme	cows	379:382	arg1	hindgut					362:368	hindgut	362:368	hindgut	362:368	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	8	104	from	series	1567:1572	arg1	metabolism					1602:1611	the metabolism	1598:1611	the metabolism of carbohydrates, and protein	1598:1641	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	10	105	from	pathways	1932:1939	arg1	metabolism					1957:1966	carbohydrate metabolism	1944:1966	carbohydrate metabolism	1944:1966	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	1	106	dep	rumen	352:356	arg1	the					348:350	the	348:350	the	348:350	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	5	107	theme	P	1012:1012	arg1	0.035					1016:1020	P = 0.035	1012:1020	P = 0.035	1012:1020	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	107	theme	P	1012:1012	arg1	concentration					997:1009	decreased milk urea nitrogen concentration	968:1009	decreased milk urea nitrogen concentration (P = 0.035)	968:1021	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	8	108	theme	Prevotellaceae	1518:1531	arg1	abundance					1490:1498	the relative abundance	1477:1498	the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level	1477:1558	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	2	109	dep	multiparous	416:426	arg1	±					440:440	±	440:440	±	440:440	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	6	110	theme	protein	1203:1209	arg1	P					1218:1218	P = 0.008	1218:1226	P = 0.008	1218:1226	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	110	theme	protein	1203:1209	arg1	yield					1211:1215	milk protein yield	1198:1215	milk protein yield (P = 0.008)	1198:1227	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	110	theme	protein	1203:1209	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	8	111	theme	genes	1446:1450	arg1	sequencing					1419:1428	Miseq sequencing	1413:1428	Miseq sequencing of the 16S rRNA genes	1413:1450	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	10	112	theme	sensu	1724:1728	arg1	0.013					1745:1749	P = 0.013	1741:1749	P = 0.013	1741:1749	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	112	theme	sensu	1724:1728	arg1	stricto					1730:1736	Clostridium sensu stricto 1	1712:1738	Clostridium sensu stricto 1 (P = 0.013)	1712:1750	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	113	theme	secondary	1969:1977	arg1	biosynthesis					1989:2000	secondary bile acid biosynthesis	1969:2000	secondary bile acid biosynthesis	1969:2000	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	12	114	from	effects	2248:2254	arg1	rumen					2277:2281	rumen	2277:2281	rumen	2277:2281	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	12	114	from	effects	2248:2254	arg1	hindgut					2287:2293	hindgut	2287:2293	hindgut	2287:2293	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	1	115	theme	bacterial	310:318	arg1	community					320:328	bacterial community	310:328	bacterial community	310:328	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	6	116	theme	=	1256:1256	arg1	yield					1147:1151	milk yield	1142:1151	milk yield (P = 0.015)	1142:1163	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	116	theme	=	1256:1256	arg1	0.030					1258:1262	P = 0.030	1254:1262	P = 0.030	1254:1262	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	2	117	dep	groups	558:563	arg1	cows					569:572	11 cows	566:572	11 cows per treatment	566:586	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	10	118	theme	=	1847:1847	arg1	P					1845:1845	P = 0.016	1845:1853	P = 0.016	1845:1853	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	118	theme	=	1847:1847	arg1	Bacteroides					1832:1842	Bacteroides	1832:1842	Bacteroides (P = 0.016)	1832:1854	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	6	119	theme	P	1102:1102	arg1	<					1104:1104	P < 0.05	1102:1109	P < 0.05	1102:1109	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	119	theme	P	1102:1102	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	5	120	theme	rectal	798:803	arg1	temperature					805:815	the rectal temperature	794:815	the rectal temperature	794:815	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	4	121	contain	contained	738:746	arg1	product					730:736	The yeast product	720:736	The yeast product	720:736	The yeast product contained 2.0 × 10 10 CFU/g.
36534956	4	121	contain	contained	738:746	arg2	CFU/g					760:764	2.0 × 10 10 CFU/g	748:764	2.0 × 10 10 CFU/g	748:764	The yeast product contained 2.0 × 10 10 CFU/g.
36534956	2	122	theme	Holstein	447:454	arg1	cows					462:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows	403:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment)	403:524	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	10	123	theme	P	1741:1741	arg1	0.013					1745:1749	P = 0.013	1741:1749	P = 0.013	1741:1749	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	123	theme	P	1741:1741	arg1	stricto					1730:1736	Clostridium sensu stricto 1	1712:1738	Clostridium sensu stricto 1 (P = 0.013)	1712:1750	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	8	124	theme	genus	1548:1552	arg1	level					1554:1558	the genus level	1544:1558	the genus level	1544:1558	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	0	125	theme	yeast	5:9	arg1	supplementation					11:25	Live yeast supplementation	0:25	Live yeast supplementation	0:25	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	5	126	theme	milk	908:911	arg1	yield					921:925	milk protein yield	908:925	milk protein yield	908:925	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	6	127	theme	lactose	1239:1245	arg1	yield					1247:1251	milk lactose yield	1234:1251	milk lactose yield	1234:1251	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	127	theme	lactose	1239:1245	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	8	128	theme	carbohydrates	1616:1628	arg1	metabolism					1602:1611	the metabolism	1598:1611	the metabolism of carbohydrates, and protein	1598:1641	Miseq sequencing of the 16S rRNA genes showed that LY increased the relative abundance of Prevotella and Prevotellaceae UCG-003 at the genus level with a series of enriched pathways in the metabolism of carbohydrates, and protein.
36534956	10	129	theme	Oscillospirales	1860:1874	arg1	P					1885:1885	P = 0.005	1885:1893	P = 0.005	1885:1893	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	129	theme	Oscillospirales	1860:1874	arg1	UCG-010					1876:1882	Oscillospirales UCG-010	1860:1882	Oscillospirales UCG-010 (P = 0.005)	1860:1894	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	1	130	from	community	320:328	arg1	rumen					352:356	rumen	352:356	rumen	352:356	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	1	130	from	community	320:328	arg1	hindgut					362:368	hindgut	362:368	hindgut	362:368	The objective of this study was to investigate the effects of live yeast (LY, Saccharomyces cerevisiae) on the lactation performance, bacterial community, and functions in the rumen and hindgut of dairy cows under heat stress.
36534956	5	131	theme	<	954:954	arg1	yield					885:889	milk yield	880:889	milk yield	880:889	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	131	theme	<	954:954	arg1	P					952:952	P < 0.001	952:960	P < 0.001	952:960	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	11	132	theme	bacterial	2046:2054	arg1	composition					2068:2078	the bacterial community's composition	2042:2078	the bacterial community's composition	2042:2078	In summary, LY supplementation altered the bacterial community's composition and function in rumen and hindgut, and simultaneously alleviated the detrimental effects of heat stress on dairy cows.
36534956	0	133	theme	bacterial	39:47	arg1	composition					61:71	bacterial community's composition	39:71	bacterial community's composition	39:71	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	5	134	theme	cows	841:844	arg1	rate					833:836	respiratory rate	821:836	respiratory rate of cows	821:844	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	134	theme	cows	841:844	arg1	temperature					805:815	the rectal temperature	794:815	the rectal temperature	794:815	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	10	135	theme	=	1887:1887	arg1	P					1885:1885	P = 0.005	1885:1893	P = 0.005	1885:1893	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	10	135	theme	=	1887:1887	arg1	UCG-010					1876:1882	Oscillospirales UCG-010	1860:1882	Oscillospirales UCG-010 (P = 0.005)	1860:1894	Clostridium sensu stricto 1 (P = 0.013) and Actinobacillus (P = 0.011) increased in the relative abundance by LY, while Bacteroides (P = 0.016) and Oscillospirales UCG-010 (P = 0.005) decreased with a series of enriched pathways in carbohydrate metabolism, secondary bile acid biosynthesis.
36534956	12	136	theme	yeast	2264:2268	arg1	effects					2248:2254	the effects	2244:2254	the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress	2244:2330	These findings provide extended insight into the effects of live yeast in the rumen and hindgut of dairy cows exposed to heat stress.
36534956	6	137	theme	P	1131:1131	arg1	<					1133:1133	P < 0.05	1131:1138	P < 0.05	1131:1138	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	137	theme	P	1131:1131	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	5	138	theme	lactose	937:943	arg1	yield					945:949	milk lactose yield	932:949	milk lactose yield	932:949	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	2	139	theme	Thirty-three	403:414	arg1	cows					462:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows	403:465	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment)	403:524	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
36534956	6	140	theme	urea	1171:1174	arg1	P					1186:1186	P = 0.001	1186:1194	P = 0.001	1186:1194	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	140	theme	urea	1171:1174	arg1	nitrogen					1176:1183	milk urea nitrogen	1166:1183	milk urea nitrogen (P = 0.001)	1166:1195	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	6	140	theme	urea	1171:1174	arg1	temperature					1089:1099	rectal temperature	1082:1099	rectal temperature (P < 0.05)	1082:1110	Interaction effects of treatment × week were observed for rectal temperature (P < 0.05), respiratory rate (P < 0.05), milk yield (P = 0.015), milk urea nitrogen (P = 0.001), milk protein yield (P = 0.008), and milk lactose yield (P = 0.030).
36534956	7	141	theme	P	1402:1402	arg1	NH3-N					1395:1399	NH3-N	1395:1399	NH3-N (P < 0.05)	1395:1410	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	7	141	theme	P	1402:1402	arg1	<					1404:1404	P < 0.05	1402:1409	P < 0.05	1402:1409	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	0	142	from	effects	138:144	arg1	cows					170:173	dairy cows	164:173	dairy cows	164:173	Live yeast supplementation altered the bacterial community's composition and function in rumen and hindgut and alleviated the detrimental effects of heat stress on dairy cows.
36534956	5	143	theme	nitrogen	988:995	arg1	0.035					1016:1020	P = 0.035	1012:1020	P = 0.035	1012:1020	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	5	143	theme	nitrogen	988:995	arg1	concentration					997:1009	decreased milk urea nitrogen concentration	968:1009	decreased milk urea nitrogen concentration (P = 0.035)	968:1021	Supplementing LY decreased the rectal temperature and respiratory rate of cows, and increased dry matter intake, milk yield, milk fat yield, milk protein yield, and milk lactose yield (P < 0.001), yet decreased milk urea nitrogen concentration (P = 0.035).
36534956	7	144	theme	NH3-N	1395:1399	arg1	concentrations					1293:1306	the concentrations	1289:1306	the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05)	1289:1410	In rumen, LY increased the concentrations of acetate, isobutyrate, isovaterate, valerate, total volatile fatty acids (VFAs), and NH3-N (P < 0.05).
36534956	2	145	from	d	480:480	arg1	milk					485:488	milk	485:488	milk	485:488	Thirty-three multiparous (parity 3.9 ± 0.8) Holstein dairy cows (189.1 ± 6.6 d in milk at the beginning of the experiment) were randomly assigned to three groups (11 cows per treatment).
35732902	5	0	theme	body	671:674	arg1	weight					676:681	body weight	671:681	body weight	671:681	METHODS C57BL/6 J mice were fed by a low-fat diet (LFD) and HFD with or without FA at a dose of 100 mg/kg of body weight by oral gavage for 12 weeks.
35732902	7	1	theme	short-chain	1048:1058	arg1	lipopolysaccharides					1348:1366	lipopolysaccharides	1348:1366	lipopolysaccharides	1348:1366	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	1	theme	short-chain	1048:1058	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	1	theme	short-chain	1048:1058	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	5	2	theme	weight	676:681	arg1	100 mg/kg					658:666	100 mg/kg	658:666	100 mg/kg of body weight by oral gavage for 12 weeks	658:709	METHODS C57BL/6 J mice were fed by a low-fat diet (LFD) and HFD with or without FA at a dose of 100 mg/kg of body weight by oral gavage for 12 weeks.
35732902	9	3	theme	FA	1696:1697	arg1	enhancement					1610:1620	enhancement	1610:1620	enhancement of intestinal barrier by altering the GM	1610:1661	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	9	3	theme	FA	1696:1697	arg1	target					1686:1691	an anti-obesity target	1670:1691	an anti-obesity target of FA	1670:1697	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	5	4	theme	low-fat	599:605	arg1	LFD					613:615	LFD	613:615	LFD	613:615	METHODS C57BL/6 J mice were fed by a low-fat diet (LFD) and HFD with or without FA at a dose of 100 mg/kg of body weight by oral gavage for 12 weeks.
35732902	5	4	theme	low-fat	599:605	arg1	diet					607:610	a low-fat diet	597:610	a low-fat diet (LFD)	597:616	METHODS C57BL/6 J mice were fed by a low-fat diet (LFD) and HFD with or without FA at a dose of 100 mg/kg of body weight by oral gavage for 12 weeks.
35732902	6	5	theme	high-throughput	718:732	arg1	sequencing					734:743	high-throughput sequencing	718:743	high-throughput sequencing	718:743	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	2	6	theme	body	319:322	arg1	weight					324:329	body weight	319:329	body weight	319:329	Ferulic acid (FA) has shown anti-obesity effects, e.g., reducing body weight and food intake.
35732902	7	7	theme	decreased	1251:1259	arg1	bacteria					1281:1288	decreased endotoxin-producing bacteria	1251:1288	decreased endotoxin-producing bacteria	1251:1288	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	7	theme	decreased	1251:1259	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	0	8	theme	diet-induced	106:117	arg1	mice					119:122	high-fat diet-induced mice	97:122	high-fat diet-induced mice	97:122	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	7	9	theme	bacterial	1309:1317	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	9	theme	bacterial	1309:1317	arg1	genera					1319:1324	obesity-related bacterial genera	1293:1324	obesity-related bacterial genera	1293:1324	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	10	theme	enhanced	1028:1035	arg1	lipopolysaccharides					1348:1366	lipopolysaccharides	1348:1366	lipopolysaccharides	1348:1366	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	10	theme	enhanced	1028:1035	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	10	theme	enhanced	1028:1035	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	0	11	theme	high-fat	97:104	arg1	mice					119:122	high-fat diet-induced mice	97:122	high-fat diet-induced mice	97:122	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	8	12	theme	intestinal	1470:1479	arg1	inflammation					1481:1492	intestinal inflammation	1470:1492	intestinal inflammation	1470:1492	Thus, FA can mitigate colonic barrier dysfunction and intestinal inflammation, induce the production of SCFAs and inhibit endotoxins by modulating the GM.
35732902	7	13	theme	intestinal	1037:1046	arg1	lipopolysaccharides					1348:1366	lipopolysaccharides	1348:1366	lipopolysaccharides	1348:1366	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	13	theme	intestinal	1037:1046	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	13	theme	intestinal	1037:1046	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	9	14	used	used	1718:1721	arg2	FA					1708:1709	FA	1708:1709	FA	1708:1709	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	9	14	used	used	1718:1721	arg2	compound					1739:1746	a functional compound	1726:1746	a functional compound with great developmental values	1726:1778	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	6	15	theme	immunohistochemical	810:828	arg1	staining					830:837	immunohistochemical staining	810:837	immunohistochemical staining	810:837	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	9	16	theme	intestinal	1625:1634	arg1	barrier					1636:1642	intestinal barrier	1625:1642	intestinal barrier	1625:1642	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	1	17	dep	PURPOSE	125:131	arg1	HFD					150:152	HFD	150:152	HFD	150:152	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	1	17	dep	PURPOSE	125:131	arg1	diet					144:147	A high-fat diet	133:147	PURPOSE A high-fat diet (HFD)	125:153	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	9	18	theme	functional	1728:1737	arg1	compound					1739:1746	a functional compound	1726:1746	a functional compound with great developmental values	1726:1778	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	9	18	theme	functional	1728:1737	arg1	FA					1708:1709	FA	1708:1709	FA	1708:1709	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	1	19	theme	gut	163:165	arg1	GM					179:180	GM	179:180	GM	179:180	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	1	19	theme	gut	163:165	arg1	microbiota					167:176	gut microbiota	163:176	gut microbiota (GM) disorders	163:191	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	9	20	theme	developmental	1759:1771	arg1	values					1773:1778	great developmental values	1753:1778	great developmental values	1753:1778	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	4	21	theme	present	449:455	arg1	study					457:461	The present study	445:461	The present study	445:461	The present study aimed to clarify the mechanism underlying the anti-obesity effects of FA and modulation of the GM.
35732902	9	22	theme	barrier	1636:1642	arg1	enhancement					1610:1620	enhancement	1610:1620	enhancement of intestinal barrier by altering the GM	1610:1661	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	9	22	theme	barrier	1636:1642	arg1	target					1686:1691	an anti-obesity target	1670:1691	an anti-obesity target of FA	1670:1697	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	4	23	theme	GM	558:559	arg1	effects					522:528	the anti-obesity effects	505:528	the anti-obesity effects of FA	505:534	The present study aimed to clarify the mechanism underlying the anti-obesity effects of FA and modulation of the GM.
35732902	4	23	theme	GM	558:559	arg1	modulation					540:549	modulation	540:549	modulation of the GM	540:559	The present study aimed to clarify the mechanism underlying the anti-obesity effects of FA and modulation of the GM.
35732902	1	24	theme	microbiota	167:176	arg1	disorders					183:191	gut microbiota (GM) disorders	163:191	gut microbiota (GM) disorders	163:191	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	7	25	theme	TLR4/NF-κB	1396:1405	arg1	pathway					1407:1413	the colonic TLR4/NF-κB pathway	1384:1413	the colonic TLR4/NF-κB pathway	1384:1413	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	26	theme	GM	1000:1001	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	26	theme	GM	1000:1001	arg1	bacteria					1281:1288	decreased endotoxin-producing bacteria	1251:1288	decreased endotoxin-producing bacteria	1251:1288	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	26	theme	GM	1000:1001	arg1	endotoxin					1337:1345	serum endotoxin	1331:1345	serum endotoxin	1331:1345	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	26	theme	GM	1000:1001	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	26	theme	GM	1000:1001	arg1	genera					1319:1324	obesity-related bacterial genera	1293:1324	obesity-related bacterial genera	1293:1324	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	5	27	theme	100 mg/kg	658:666	arg1	dose					650:653	a dose	648:653	a dose of 100 mg/kg of body weight by oral gavage for 12 weeks	648:709	METHODS C57BL/6 J mice were fed by a low-fat diet (LFD) and HFD with or without FA at a dose of 100 mg/kg of body weight by oral gavage for 12 weeks.
35732902	9	28	with	compound	1739:1746	arg1	values					1773:1778	great developmental values	1753:1778	great developmental values	1753:1778	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	9	29	theme	anti-obesity	1673:1684	arg1	enhancement					1610:1620	enhancement	1610:1620	enhancement of intestinal barrier by altering the GM	1610:1661	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	9	29	theme	anti-obesity	1673:1684	arg1	target					1686:1691	an anti-obesity target	1670:1691	an anti-obesity target of FA	1670:1697	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	3	30	theme	anti-obesity	383:394	arg1	effects					396:402	the anti-obesity effects	379:402	the anti-obesity effects of FA and GM modulation	379:426	However, the mechanism linking the anti-obesity effects of FA and GM modulation remains obscure.
35732902	0	31	theme	Ferulic	0:6	arg1	acid					8:11	Ferulic acid	0:11	Ferulic acid	0:11	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	6	32	theme	barrier	902:908	arg1	integrity					910:918	intestinal barrier integrity	891:918	intestinal barrier integrity	891:918	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	2	33	theme	anti-obesity	282:293	arg1	effects					295:301	anti-obesity effects	282:301	anti-obesity effects	282:301	Ferulic acid (FA) has shown anti-obesity effects, e.g., reducing body weight and food intake.
35732902	9	34	theme	great	1753:1757	arg1	values					1773:1778	great developmental values	1753:1778	great developmental values	1753:1778	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	6	35	theme	fluorescence	776:787	arg1	PCR					802:804	real-time fluorescence quantitative PCR	766:804	real-time fluorescence quantitative PCR	766:804	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	0	36	theme	intestinal	22:31	arg1	function					41:48	intestinal barrier function	22:48	intestinal barrier function	22:48	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	4	37	theme	anti-obesity	509:520	arg1	effects					522:528	the anti-obesity effects	505:528	the anti-obesity effects of FA	505:534	The present study aimed to clarify the mechanism underlying the anti-obesity effects of FA and modulation of the GM.
35732902	4	38	theme	FA	533:534	arg1	effects					522:528	the anti-obesity effects	505:528	the anti-obesity effects of FA	505:534	The present study aimed to clarify the mechanism underlying the anti-obesity effects of FA and modulation of the GM.
35732902	4	38	theme	FA	533:534	arg1	modulation					540:549	modulation	540:549	modulation of the GM	540:559	The present study aimed to clarify the mechanism underlying the anti-obesity effects of FA and modulation of the GM.
35732902	7	39	theme	HFD-induced	988:998	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	39	theme	HFD-induced	988:998	arg1	bacteria					1281:1288	decreased endotoxin-producing bacteria	1251:1288	decreased endotoxin-producing bacteria	1251:1288	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	39	theme	HFD-induced	988:998	arg1	endotoxin					1337:1345	serum endotoxin	1331:1345	serum endotoxin	1331:1345	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	39	theme	HFD-induced	988:998	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	39	theme	HFD-induced	988:998	arg1	genera					1319:1324	obesity-related bacterial genera	1293:1324	obesity-related bacterial genera	1293:1324	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	6	40	theme	quantitative	789:800	arg1	PCR					802:804	real-time fluorescence quantitative PCR	766:804	real-time fluorescence quantitative PCR	766:804	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	7	41	theme	SCFA	1072:1075	arg1	lipopolysaccharides					1348:1366	lipopolysaccharides	1348:1366	lipopolysaccharides	1348:1366	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	41	theme	SCFA	1072:1075	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	41	theme	SCFA	1072:1075	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	0	42	from	composition	82:92	arg1	mice					119:122	high-fat diet-induced mice	97:122	high-fat diet-induced mice	97:122	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	0	43	theme	barrier	33:39	arg1	function					41:48	intestinal barrier function	22:48	intestinal barrier function	22:48	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	5	44	theme	oral	686:689	arg1	gavage					691:696	oral gavage	686:696	oral gavage for 12 weeks	686:709	METHODS C57BL/6 J mice were fed by a low-fat diet (LFD) and HFD with or without FA at a dose of 100 mg/kg of body weight by oral gavage for 12 weeks.
35732902	6	45	theme	real-time	766:774	arg1	PCR					802:804	real-time fluorescence quantitative PCR	766:804	real-time fluorescence quantitative PCR	766:804	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	7	46	theme	serum	1331:1335	arg1	endotoxin					1337:1345	serum endotoxin	1331:1345	serum endotoxin	1331:1345	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	46	theme	serum	1331:1335	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	3	47	theme	GM	414:415	arg1	effects					396:402	the anti-obesity effects	379:402	the anti-obesity effects of FA and GM modulation	379:426	However, the mechanism linking the anti-obesity effects of FA and GM modulation remains obscure.
35732902	7	48	theme	acid	1066:1069	arg1	lipopolysaccharides					1348:1366	lipopolysaccharides	1348:1366	lipopolysaccharides	1348:1366	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	48	theme	acid	1066:1069	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	48	theme	acid	1066:1069	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	8	49	theme	SCFAs	1520:1524	arg1	production					1506:1515	the production	1502:1515	the production of SCFAs	1502:1524	Thus, FA can mitigate colonic barrier dysfunction and intestinal inflammation, induce the production of SCFAs and inhibit endotoxins by modulating the GM.
35732902	6	50	theme	intestinal	891:900	arg1	integrity					910:918	intestinal barrier integrity	891:918	intestinal barrier integrity	891:918	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	7	51	theme	colonic	1388:1394	arg1	pathway					1407:1413	the colonic TLR4/NF-κB pathway	1384:1413	the colonic TLR4/NF-κB pathway	1384:1413	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	52	theme	SCFA	1202:1205	arg1	accumulation					1207:1218	SCFA accumulation	1202:1218	SCFA accumulation	1202:1218	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	8	53	theme	barrier	1446:1452	arg1	dysfunction					1454:1464	colonic barrier dysfunction	1438:1464	colonic barrier dysfunction	1438:1464	Thus, FA can mitigate colonic barrier dysfunction and intestinal inflammation, induce the production of SCFAs and inhibit endotoxins by modulating the GM.
35732902	7	54	theme	weight	966:971	arg1	gain					973:976	weight gain	966:976	weight gain	966:976	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	3	55	dep	FA	407:408	arg1	modulation					417:426	modulation	417:426	modulation	417:426	However, the mechanism linking the anti-obesity effects of FA and GM modulation remains obscure.
35732902	7	56	theme	endotoxin-producing	1261:1279	arg1	bacteria					1281:1288	decreased endotoxin-producing bacteria	1251:1288	decreased endotoxin-producing bacteria	1251:1288	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	56	theme	endotoxin-producing	1261:1279	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	57	theme	fatty	1060:1064	arg1	lipopolysaccharides					1348:1366	lipopolysaccharides	1348:1366	lipopolysaccharides	1348:1366	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	57	theme	fatty	1060:1064	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	57	theme	fatty	1060:1064	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	6	58	theme	obesity	859:865	arg1	attenuation					844:854	the attenuation	840:854	the attenuation of obesity by FA	840:871	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	7	59	theme	RESULTS	947:953	arg1	FA					955:956	RESULTS FA	947:956	RESULTS FA	947:956	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	6	60	theme	gas	746:748	arg1	chromatography					750:763	gas chromatography	746:763	gas chromatography	746:763	Using high-throughput sequencing, gas chromatography, real-time fluorescence quantitative PCR and immunohistochemical staining, the attenuation of obesity by FA were assessed via intestinal barrier integrity, inflammation, and the GM.
35732902	2	61	theme	Ferulic	254:260	arg1	FA					268:269	FA	268:269	FA	268:269	Ferulic acid (FA) has shown anti-obesity effects, e.g., reducing body weight and food intake.
35732902	2	61	theme	Ferulic	254:260	arg1	acid					262:265	Ferulic acid	254:265	Ferulic acid (FA)	254:270	Ferulic acid (FA) has shown anti-obesity effects, e.g., reducing body weight and food intake.
35732902	1	62	theme	high-fat	135:142	arg1	HFD					150:152	HFD	150:152	HFD	150:152	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	1	62	theme	high-fat	135:142	arg1	diet					144:147	A high-fat diet	133:147	PURPOSE A high-fat diet (HFD)	125:153	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	7	63	dep	Eisenbergiella	1115:1128	arg1	e.g.					1098:1101	e.g.	1098:1101	e.g.	1098:1101	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	64	theme	obesity-related	1293:1307	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	64	theme	obesity-related	1293:1307	arg1	genera					1319:1324	obesity-related bacterial genera	1293:1324	obesity-related bacterial genera	1293:1324	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	65	theme	-producing	1077:1086	arg1	lipopolysaccharides					1348:1366	lipopolysaccharides	1348:1366	lipopolysaccharides	1348:1366	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	65	theme	-producing	1077:1086	arg1	bacteria					1088:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria	1037:1095	intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression	1037:1248	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	7	65	theme	-producing	1077:1086	arg1	imbalance					1003:1011	HFD-induced GM imbalance	988:1011	HFD-induced GM imbalance	988:1011	RESULTS FA reduced weight gain, improved HFD-induced GM imbalance, significantly enhanced intestinal short-chain fatty acid (SCFA)-producing bacteria (e.g., Olsenella, Eisenbergiella, Dubosiella, Clostridiales_unclassified, and Faecalibaculum) along with SCFA accumulation and its receptors' expression, decreased endotoxin-producing bacteria or obesity-related bacterial genera, and serum endotoxin (lipopolysaccharides), and inhibited the colonic TLR4/NF-κB pathway.
35732902	1	66	theme	intestinal	205:214	arg1	dysfunction					224:234	intestinal barrier dysfunction	205:234	intestinal barrier dysfunction	205:234	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	0	67	theme	microbiota	71:80	arg1	composition					82:92	gut microbiota composition	67:92	gut microbiota composition in high-fat diet-induced mice	67:122	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	9	68	dep	CONCLUSION	1571:1580	arg1	indicate					1596:1603	indicate	1596:1603	indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values	1596:1778	CONCLUSION These results indicate that enhancement of intestinal barrier by altering the GM may be an anti-obesity target of FA and that FA can be used as a functional compound with great developmental values.
35732902	2	69	theme	food	335:338	arg1	intake					340:345	food intake	335:345	food intake	335:345	Ferulic acid (FA) has shown anti-obesity effects, e.g., reducing body weight and food intake.
35732902	3	70	theme	FA	407:408	arg1	effects					396:402	the anti-obesity effects	379:402	the anti-obesity effects of FA and GM modulation	379:426	However, the mechanism linking the anti-obesity effects of FA and GM modulation remains obscure.
35732902	1	71	theme	barrier	216:222	arg1	dysfunction					224:234	intestinal barrier dysfunction	205:234	intestinal barrier dysfunction	205:234	PURPOSE A high-fat diet (HFD) induces gut microbiota (GM) disorders, leading to intestinal barrier dysfunction and inflammation.
35732902	0	72	theme	gut	67:69	arg1	composition					82:92	gut microbiota composition	67:92	gut microbiota composition in high-fat diet-induced mice	67:122	Ferulic acid improves intestinal barrier function through altering gut microbiota composition in high-fat diet-induced mice.
35732902	8	73	theme	colonic	1438:1444	arg1	dysfunction					1454:1464	colonic barrier dysfunction	1438:1464	colonic barrier dysfunction	1438:1464	Thus, FA can mitigate colonic barrier dysfunction and intestinal inflammation, induce the production of SCFAs and inhibit endotoxins by modulating the GM.
37140807	9	0	theme	exploring	1197:1205	arg1	activities					1180:1189	biological activities	1169:1189	biological activities worth exploring	1169:1205	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	8	1	theme	biological	953:962	arg1	properties					964:973	biological properties	953:973	biological properties	953:973	EPS stood out due to its high content of fucose (40.9 mol%), a sugar known to confer biological properties to polysaccharides.
37140807	9	2	contain	have	1164:1167	arg2	activities					1180:1189	biological activities	1169:1189	biological activities worth exploring	1169:1205	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	9	2	contain	have	1164:1167	arg1	EPS					1154:1156	these EPS	1148:1156	these EPS	1148:1156	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	4	3	theme	EPS	592:594	arg1	yield					596:600	the highest EPS yield	580:600	the highest EPS yield	580:600	All strains were found to be EPS producers, though the highest EPS yield was obtained for Tisochrysis lutea, followed by Heterocapsa sp.
37140807	5	4	dep	L-1	685:687	arg1	mg					682:683	mg	682:683	mg	682:683	(126.8 and 75.8 mg L-1, respectively).
37140807	8	5	theme	mol	922:924	arg1	fucose					909:914	fucose	909:914	fucose (40.9 mol%)	909:926	EPS stood out due to its high content of fucose (40.9 mol%), a sugar known to confer biological properties to polysaccharides.
37140807	8	5	theme	mol	922:924	arg1	%					925:925	40.9 mol%	917:925	40.9 mol%	917:925	EPS stood out due to its high content of fucose (40.9 mol%), a sugar known to confer biological properties to polysaccharides.
37140807	7	6	theme	Heterocapsa	852:862	arg1	sp					864:865	Heterocapsa sp	852:865	Heterocapsa sp.	852:866	Heterocapsa sp.
37140807	3	7	theme	different	408:416	arg1	lineages					418:425	three different lineages	402:425	three different lineages	402:425	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	8	from	lineages	418:425	arg1	producers					518:526	EPS producers	514:526	EPS producers	514:526	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	8	from	lineages	418:425	arg1	Dinophyceae					435:445	Dinophyceae	435:445	Dinophyceae	435:445	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	8	from	lineages	418:425	arg1	Chlorophyta					480:490	Chlorophyta	480:490	Chlorophyta	480:490	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	8	from	lineages	418:425	arg1	Haptophyta					464:473	Haptophyta	464:473	Haptophyta	464:473	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	8	from	lineages	418:425	arg1	strains					389:395	Seven microalgae strains	372:395	Seven microalgae strains from three different lineages	372:425	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	5	9	dep	mg	682:683	arg1	75.8					677:680	75.8	677:680	75.8	677:680	(126.8 and 75.8 mg L-1, respectively).
37140807	5	9	dep	mg	682:683	arg1	126.8					667:671	126.8	667:671	126.8	667:671	(126.8 and 75.8 mg L-1, respectively).
37140807	6	10	theme	sugars	792:797	arg1	rhamnose					818:825	rhamnose	818:825	rhamnose	818:825	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	6	10	theme	sugars	792:797	arg1	contents					772:779	significant contents	760:779	significant contents	760:779	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	6	10	theme	sugars	792:797	arg1	ribose					832:837	ribose	832:837	ribose	832:837	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	6	10	theme	sugars	792:797	arg1	fucose					810:815	fucose	810:815	fucose	810:815	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	9	11	theme	sulfate	1011:1017	arg1	groups					1019:1024	sulfate groups	1011:1024	sulfate groups (10.6-33.5 wt%)	1011:1040	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	9	11	theme	sulfate	1011:1017	arg1	%					1039:1039	10.6-33.5 wt%	1027:1039	10.6-33.5 wt%	1027:1039	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	0	12	theme	marine	65:70	arg1	origin					72:77	marine origin	65:77	marine origin	65:77	Bioprospecting for new exopolysaccharide-producing microalgae of marine origin.
37140807	6	13	theme	unusual	784:790	arg1	sugars					792:797	unusual sugars	784:797	unusual sugars	784:797	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	1	14	theme	photosynthetic	95:108	arg1	Microalgae					80:89	Microalgae	80:89	Microalgae	80:89	Microalgae are photosynthetic organisms that can produce biomolecules with industrial interest, including exopolysaccharides (EPS).
37140807	1	14	theme	photosynthetic	95:108	arg1	organisms					110:118	photosynthetic organisms	95:118	photosynthetic organisms that can produce biomolecules with industrial interest, including exopolysaccharides (EPS)	95:209	Microalgae are photosynthetic organisms that can produce biomolecules with industrial interest, including exopolysaccharides (EPS).
37140807	9	15	theme	groups	1019:1024	arg1	presence					999:1006	The presence	995:1006	The presence of sulfate groups (10.6-33.5 wt%)	995:1040	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	1	16	with	biomolecules	137:148	arg1	interest					166:173	industrial interest	155:173	industrial interest	155:173	Microalgae are photosynthetic organisms that can produce biomolecules with industrial interest, including exopolysaccharides (EPS).
37140807	2	17	theme	therapeutic	353:363	arg1	areas					365:369	cosmetic and/or therapeutic areas	337:369	cosmetic and/or therapeutic areas	337:369	Due to their structural and compositional diversity, microalgae EPS present interesting properties that can be considered in cosmetic and/or therapeutic areas.
37140807	9	18	theme	microalgae	1086:1095	arg1	strains					1097:1103	all microalgae strains	1082:1103	all microalgae strains	1082:1103	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	0	19	theme	exopolysaccharide-producing	23:49	arg1	microalgae					51:60	new exopolysaccharide-producing microalgae	19:60	new exopolysaccharide-producing microalgae of marine origin	19:77	Bioprospecting for new exopolysaccharide-producing microalgae of marine origin.
37140807	6	20	theme	significant	760:770	arg1	rhamnose					818:825	rhamnose	818:825	rhamnose	818:825	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	6	20	theme	significant	760:770	arg1	contents					772:779	significant contents	760:779	significant contents	760:779	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	6	20	theme	significant	760:770	arg1	ribose					832:837	ribose	832:837	ribose	832:837	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	6	20	theme	significant	760:770	arg1	fucose					810:815	fucose	810:815	fucose	810:815	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	2	21	theme	cosmetic	337:344	arg1	areas					365:369	cosmetic and/or therapeutic areas	337:369	cosmetic and/or therapeutic areas	337:369	Due to their structural and compositional diversity, microalgae EPS present interesting properties that can be considered in cosmetic and/or therapeutic areas.
37140807	9	22	theme	wt	1037:1038	arg1	groups					1019:1024	sulfate groups	1011:1024	sulfate groups (10.6-33.5 wt%)	1011:1040	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	9	22	theme	wt	1037:1038	arg1	%					1039:1039	10.6-33.5 wt%	1027:1039	10.6-33.5 wt%	1027:1039	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	0	23	theme	new	19:21	arg1	microalgae					51:60	new exopolysaccharide-producing microalgae	19:60	new exopolysaccharide-producing microalgae of marine origin	19:77	Bioprospecting for new exopolysaccharide-producing microalgae of marine origin.
37140807	4	24	theme	highest	584:590	arg1	yield					596:600	the highest EPS yield	580:600	the highest EPS yield	580:600	All strains were found to be EPS producers, though the highest EPS yield was obtained for Tisochrysis lutea, followed by Heterocapsa sp.
37140807	2	25	theme	microalgae	265:274	arg1	EPS					276:278	microalgae EPS	265:278	microalgae EPS	265:278	Due to their structural and compositional diversity, microalgae EPS present interesting properties that can be considered in cosmetic and/or therapeutic areas.
37140807	4	26	theme	Heterocapsa	650:660	arg1	sp					662:663	Heterocapsa sp	650:663	Heterocapsa sp	650:663	All strains were found to be EPS producers, though the highest EPS yield was obtained for Tisochrysis lutea, followed by Heterocapsa sp.
37140807	0	27	theme	origin	72:77	arg1	microalgae					51:60	new exopolysaccharide-producing microalgae	19:60	new exopolysaccharide-producing microalgae of marine origin	19:77	Bioprospecting for new exopolysaccharide-producing microalgae of marine origin.
37140807	6	28	theme	composition	747:757	arg1	assessment					710:719	assessment	710:719	assessment of the polymers' chemical composition	710:757	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	3	29	theme	phylum	448:453	arg1	Dinophyceae					435:445	Dinophyceae	435:445	Dinophyceae	435:445	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	29	theme	phylum	448:453	arg1	Miozoa					455:460	phylum Miozoa	448:460	phylum Miozoa	448:460	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	2	30	theme	compositional	240:252	arg1	diversity					254:262	their structural and compositional diversity	219:262	their structural and compositional diversity	219:262	Due to their structural and compositional diversity, microalgae EPS present interesting properties that can be considered in cosmetic and/or therapeutic areas.
37140807	6	31	theme	chemical	738:745	arg1	composition					747:757	the polymers' chemical composition	724:757	the polymers' chemical composition	724:757	Upon assessment of the polymers' chemical composition, significant contents of unusual sugars, including fucose, rhamnose, and ribose, were found.
37140807	4	32	theme	EPS	558:560	arg1	producers					562:570	EPS producers	558:570	EPS producers	558:570	All strains were found to be EPS producers, though the highest EPS yield was obtained for Tisochrysis lutea, followed by Heterocapsa sp.
37140807	2	33	theme	structural	225:234	arg1	diversity					254:262	their structural and compositional diversity	219:262	their structural and compositional diversity	219:262	Due to their structural and compositional diversity, microalgae EPS present interesting properties that can be considered in cosmetic and/or therapeutic areas.
37140807	8	34	theme	high	893:896	arg1	content					898:904	its high content	889:904	its high content of fucose (40.9 mol%), a sugar known to confer biological properties to polysaccharides	889:992	EPS stood out due to its high content of fucose (40.9 mol%), a sugar known to confer biological properties to polysaccharides.
37140807	1	35	theme	industrial	155:164	arg1	interest					166:173	industrial interest	155:173	industrial interest	155:173	Microalgae are photosynthetic organisms that can produce biomolecules with industrial interest, including exopolysaccharides (EPS).
37140807	4	36	theme	Tisochrysis	619:629	arg1	lutea					631:635	Tisochrysis lutea	619:635	Tisochrysis lutea	619:635	All strains were found to be EPS producers, though the highest EPS yield was obtained for Tisochrysis lutea, followed by Heterocapsa sp.
37140807	9	37	theme	biological	1169:1178	arg1	activities					1180:1189	biological activities	1169:1189	biological activities worth exploring	1169:1205	The presence of sulfate groups (10.6-33.5 wt%) was also noticed in the EPS produced by all microalgae strains, thus contributing to the possibility that these EPS might have biological activities worth exploring.
37140807	2	38	theme	interesting	288:298	arg1	properties					300:309	interesting properties	288:309	interesting properties that can be considered in cosmetic and/or therapeutic areas	288:369	Due to their structural and compositional diversity, microalgae EPS present interesting properties that can be considered in cosmetic and/or therapeutic areas.
37140807	8	39	theme	fucose	909:914	arg1	content					898:904	its high content	889:904	its high content of fucose (40.9 mol%), a sugar known to confer biological properties to polysaccharides	889:992	EPS stood out due to its high content of fucose (40.9 mol%), a sugar known to confer biological properties to polysaccharides.
37140807	3	40	theme	EPS	514:516	arg1	producers					518:526	EPS producers	514:526	EPS producers	514:526	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	40	theme	EPS	514:516	arg1	strains					389:395	Seven microalgae strains	372:395	Seven microalgae strains from three different lineages	372:425	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	41	theme	microalgae	378:387	arg1	producers					518:526	EPS producers	514:526	EPS producers	514:526	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	41	theme	microalgae	378:387	arg1	Dinophyceae					435:445	Dinophyceae	435:445	Dinophyceae	435:445	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	41	theme	microalgae	378:387	arg1	Chlorophyta					480:490	Chlorophyta	480:490	Chlorophyta	480:490	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	41	theme	microalgae	378:387	arg1	Haptophyta					464:473	Haptophyta	464:473	Haptophyta	464:473	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
37140807	3	41	theme	microalgae	378:387	arg1	strains					389:395	Seven microalgae strains	372:395	Seven microalgae strains from three different lineages	372:425	Seven microalgae strains from three different lineages, namely Dinophyceae (phylum Miozoa), Haptophyta, and Chlorophyta, were investigated as EPS producers.
35060590	0	0	theme	gut	83:85	arg1	inflammation					87:98	gut inflammation	83:98	gut inflammation	83:98	Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation.
35060590	4	1	dep	cytokines	958:966	arg1	IL-17					993:997	IL-17	993:997	IL-17	993:997	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	1	dep	cytokines	958:966	arg1	IL-1β					976:980	IL-1β	976:980	IL-1β	976:980	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	1	dep	cytokines	958:966	arg1	TNF-α					969:973	TNF-α	969:973	TNF-α	969:973	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	1	dep	cytokines	958:966	arg1	cytokines					958:966	proinflammatory cytokines	942:966	proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05)	942:1009	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	1	dep	cytokines	958:966	arg1	IL-6					983:986	IL-6	983:986	IL-6	983:986	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	0	2	with	mice	38:41	arg1	osteoporosis					48:59	osteoporosis	48:59	osteoporosis caused by colitis	48:77	Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation.
35060590	1	3	theme	bowel	168:172	arg1	disease					174:180	inflammatory bowel disease	155:180	inflammatory bowel disease	155:180	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	4	4	theme	cytokines	958:966	arg1	scores					912:917	disease activity index scores	889:917	disease activity index scores	889:917	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	4	theme	cytokines	958:966	arg1	loss					872:875	colitis-associated weight loss	846:875	colitis-associated weight loss (P < 0.05)	846:886	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	4	theme	cytokines	958:966	arg1	<					880:880	P < 0.05	878:885	P < 0.05	878:885	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	4	theme	cytokines	958:966	arg1	production					928:937	the production	924:937	the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05)	924:1009	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	1	5	theme	changes	265:271	arg1	consequence					250:260	a consequence	248:260	a consequence of changes in the intestinal microenvironment and consequent dysbiosis	248:331	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	5	6	from	reductions	1081:1090	arg1	severity					1109:1116	tissue injury severity	1095:1116	tissue injury severity	1095:1116	Colon tissue pathological sections similarly revealed BL-99-mediated reductions in tissue injury severity.
35060590	6	7	theme	tomography	1134:1143	arg1	analyses					1156:1163	Micro-computed tomography (Micro-CT) analyses	1119:1163	Micro-computed tomography (Micro-CT) analyses	1119:1163	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	8	8	theme	intestinal	1494:1503	arg1	Claudin-1					1531:1539	Claudin-1	1531:1539	Claudin-1	1531:1539	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	8	theme	intestinal	1494:1503	arg1	proteins					1521:1528	intestinal barrier-related proteins	1494:1528	intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin)	1494:1566	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	8	theme	intestinal	1494:1503	arg1	ZO-1					1548:1551	ZO-1	1548:1551	ZO-1	1548:1551	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	8	theme	intestinal	1494:1503	arg1	Occludin					1558:1565	Occludin	1558:1565	Occludin	1558:1565	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	8	theme	intestinal	1494:1503	arg1	MUC2					1542:1545	MUC2	1542:1545	MUC2	1542:1545	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	9	9	theme	cytokine	1807:1814	arg1	production					1816:1825	inflammatory cytokine production	1794:1825	inflammatory cytokine production	1794:1825	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	7	10	theme	pronounced	1367:1376	arg1	changes					1378:1384	pronounced changes	1367:1384	pronounced changes in the composition of the gut microbiota	1367:1425	Such probiotic supplementation also resulted in pronounced changes in the composition of the gut microbiota.
35060590	4	11	theme	disease	889:895	arg1	scores					912:917	disease activity index scores	889:917	disease activity index scores	889:917	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	7	12	from	changes	1378:1384	arg1	composition					1393:1403	the composition	1389:1403	the composition of the gut microbiota	1389:1425	Such probiotic supplementation also resulted in pronounced changes in the composition of the gut microbiota.
35060590	9	13	theme	UC	1710:1711	arg1	patients					1713:1720	UC patients	1710:1720	UC patients owing to its ability to shape the intestinal microflora	1710:1776	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	9	14	theme	osteoporosis	1694:1705	arg1	incidence					1681:1689	the incidence	1677:1689	the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora	1677:1776	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	3	15	theme	administration	560:573	arg1	ability					520:526	the ability	516:526	the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC)	516:685	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	7	16	theme	microbiota	1416:1425	arg1	composition					1393:1403	the composition	1389:1403	the composition of the gut microbiota	1389:1425	Such probiotic supplementation also resulted in pronounced changes in the composition of the gut microbiota.
35060590	1	17	theme	intestinal	280:289	arg1	microenvironment					291:306	the intestinal microenvironment	276:306	the intestinal microenvironment	276:306	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	0	18	theme	microbiota	108:117	arg1	regulation					119:128	gut microbiota regulation	104:128	gut microbiota regulation	104:128	Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation.
35060590	6	19	theme	significant	1183:1193	arg1	improvements					1195:1206	significant improvements	1183:1206	significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05)	1183:1316	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	4	20	theme	index	906:910	arg1	scores					912:917	disease activity index scores	889:917	disease activity index scores	889:917	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	3	21	theme	bone	594:597	arg1	loss					599:602	bone loss	594:602	bone loss	594:602	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	6	22	from	volume	1224:1229	arg1	animals					1299:1305	BL-99-treated animals	1285:1305	BL-99-treated animals	1285:1305	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	23	theme	BL-99-treated	1285:1297	arg1	animals					1299:1305	BL-99-treated animals	1285:1305	BL-99-treated animals	1285:1305	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	0	24	theme	gut	104:106	arg1	regulation					119:128	gut microbiota regulation	104:128	gut microbiota regulation	104:128	Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation.
35060590	1	25	from	dysbiosis	323:331	arg1	consequence					250:260	a consequence	248:260	a consequence of changes in the intestinal microenvironment and consequent dysbiosis	248:331	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	4	26	theme	colitis-associated	846:863	arg1	loss					872:875	colitis-associated weight loss	846:875	colitis-associated weight loss (P < 0.05)	846:886	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	26	theme	colitis-associated	846:863	arg1	<					880:880	P < 0.05	878:885	P < 0.05	878:885	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	1	27	theme	related	185:191	arg1	conditions					193:202	related conditions	185:202	related conditions	185:202	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	0	28	theme	lactis	16:21	arg1	BL-99					23:27	Bifidobacterium lactis BL-99	0:27	Bifidobacterium lactis BL-99	0:27	Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation.
35060590	9	29	theme	inflammatory	1794:1805	arg1	production					1816:1825	inflammatory cytokine production	1794:1825	inflammatory cytokine production	1794:1825	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	1	30	from	microenvironment	291:306	arg1	consequence					250:260	a consequence	248:260	a consequence of changes in the intestinal microenvironment and consequent dysbiosis	248:331	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	6	31	theme	bone	1219:1222	arg1	BV/TV					1232:1236	BV/TV	1232:1236	BV/TV	1232:1236	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	31	theme	bone	1219:1222	arg1	volume					1224:1229	percent bone volume	1211:1229	percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05)	1211:1316	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	0	32	theme	Bifidobacterium	0:14	arg1	BL-99					23:27	Bifidobacterium lactis BL-99	0:27	Bifidobacterium lactis BL-99	0:27	Bifidobacterium lactis BL-99 protects mice with osteoporosis caused by colitis via gut inflammation and gut microbiota regulation.
35060590	6	33	theme	P	1308:1308	arg1	number					1261:1266	trabecular number	1250:1266	trabecular number	1250:1266	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	33	theme	P	1308:1308	arg1	<					1310:1310	P < 0.05	1308:1315	P < 0.05	1308:1315	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	5	34	theme	tissue	1095:1100	arg1	severity					1109:1116	tissue injury severity	1095:1116	tissue injury severity	1095:1116	Colon tissue pathological sections similarly revealed BL-99-mediated reductions in tissue injury severity.
35060590	3	35	theme	dextran	632:638	arg1	sodium					640:645	dextran sodium	632:645	dextran sodium sulfate-induced ulcerative colitis (UC)	632:685	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	2	36	theme	intestinal	426:435	arg1	inflammation					437:448	intestinal inflammation	426:448	intestinal inflammation	426:448	We hypothesized that anti-inflammatory probiotic treatment would be sufficient to alleviate intestinal inflammation and thereby prevent the development of osteoporosis.
35060590	9	37	used	utilized	1619:1626	arg2	BL-99					1606:1610	BL-99	1606:1610	BL-99	1606:1610	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	9	37	used	utilized	1619:1626	arg2	preparation					1654:1664	a beneficial probiotic preparation	1631:1664	a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production	1631:1825	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	4	38	theme	analyses	791:798	arg1	results					774:780	The results	770:780	The results of these analyses	770:798	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	7	39	theme	gut	1412:1414	arg1	microbiota					1416:1425	the gut microbiota	1408:1425	the gut microbiota	1408:1425	Such probiotic supplementation also resulted in pronounced changes in the composition of the gut microbiota.
35060590	3	40	theme	experimental	610:621	arg1	model					623:627	an experimental model	607:627	an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC)	607:685	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	5	41	theme	pathological	1025:1036	arg1	sections					1038:1045	Colon tissue pathological sections	1012:1045	Colon tissue pathological sections	1012:1045	Colon tissue pathological sections similarly revealed BL-99-mediated reductions in tissue injury severity.
35060590	2	42	theme	osteoporosis	489:500	arg1	development					474:484	the development	470:484	the development of osteoporosis	470:500	We hypothesized that anti-inflammatory probiotic treatment would be sufficient to alleviate intestinal inflammation and thereby prevent the development of osteoporosis.
35060590	1	43	theme	consequent	312:321	arg1	dysbiosis					323:331	consequent dysbiosis	312:331	consequent dysbiosis	312:331	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	4	44	theme	weight	865:870	arg1	loss					872:875	colitis-associated weight loss	846:875	colitis-associated weight loss (P < 0.05)	846:886	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	44	theme	weight	865:870	arg1	<					880:880	P < 0.05	878:885	P < 0.05	878:885	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	5	45	theme	injury	1102:1107	arg1	severity					1109:1116	tissue injury severity	1095:1116	tissue injury severity	1095:1116	Colon tissue pathological sections similarly revealed BL-99-mediated reductions in tissue injury severity.
35060590	9	46	from	incidence	1681:1689	arg1	patients					1713:1720	UC patients	1710:1720	UC patients owing to its ability to shape the intestinal microflora	1710:1776	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	6	47	from	improvements	1195:1206	arg1	<					1310:1310	P < 0.05	1308:1315	P < 0.05	1308:1315	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	47	from	improvements	1195:1206	arg1	BV/TV					1232:1236	BV/TV	1232:1236	BV/TV	1232:1236	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	47	from	improvements	1195:1206	arg1	volume					1224:1229	percent bone volume	1211:1229	percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05)	1211:1316	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	47	from	improvements	1195:1206	arg1	number					1261:1266	trabecular number	1250:1266	trabecular number	1250:1266	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	48	theme	percent	1211:1217	arg1	BV/TV					1232:1236	BV/TV	1232:1236	BV/TV	1232:1236	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	48	theme	percent	1211:1217	arg1	volume					1224:1229	percent bone volume	1211:1229	percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05)	1211:1316	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	9	49	dep	preparation	1654:1664	arg1	suppress					1785:1792	suppress	1785:1792	to suppress inflammatory cytokine production	1782:1825	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	9	49	dep	preparation	1654:1664	arg1	prevent					1669:1675	prevent	1669:1675	to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora	1666:1776	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	1	50	from	changes	265:271	arg1	microenvironment					291:306	the intestinal microenvironment	276:306	the intestinal microenvironment	276:306	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	1	50	from	changes	265:271	arg1	dysbiosis					323:331	consequent dysbiosis	312:331	consequent dysbiosis	312:331	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	3	51	theme	ulcerative	663:672	arg1	UC					683:684	UC	683:684	UC	683:684	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	3	51	theme	ulcerative	663:672	arg1	colitis					674:680	ulcerative colitis	663:680	ulcerative colitis (UC)	663:685	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	6	52	from	thickness	1272:1280	arg1	animals					1299:1305	BL-99-treated animals	1285:1305	BL-99-treated animals	1285:1305	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	7	53	theme	Such	1319:1322	arg1	supplementation					1334:1348	Such probiotic supplementation	1319:1348	Such probiotic supplementation	1319:1348	Such probiotic supplementation also resulted in pronounced changes in the composition of the gut microbiota.
35060590	6	54	theme	Micro-computed	1119:1132	arg1	Micro-CT					1146:1153	Micro-CT	1146:1153	Micro-CT	1146:1153	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	54	theme	Micro-computed	1119:1132	arg1	tomography					1134:1143	Micro-computed tomography	1119:1143	Micro-computed tomography (Micro-CT) analyses	1119:1163	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	8	55	dep	proteins	1521:1528	arg1	Claudin-1					1531:1539	Claudin-1	1531:1539	Claudin-1	1531:1539	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	55	dep	proteins	1521:1528	arg1	proteins					1521:1528	intestinal barrier-related proteins	1494:1528	intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin)	1494:1566	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	55	dep	proteins	1521:1528	arg1	ZO-1					1548:1551	ZO-1	1548:1551	ZO-1	1548:1551	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	55	dep	proteins	1521:1528	arg1	Occludin					1558:1565	Occludin	1558:1565	Occludin	1558:1565	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	55	dep	proteins	1521:1528	arg1	MUC2					1542:1545	MUC2	1542:1545	MUC2	1542:1545	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	56	theme	proteins	1521:1528	arg1	expression					1480:1489	the expression	1476:1489	the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin)	1476:1566	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	6	57	from	number	1261:1266	arg1	animals					1299:1305	BL-99-treated animals	1285:1305	BL-99-treated animals	1285:1305	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	7	58	theme	probiotic	1324:1332	arg1	supplementation					1334:1348	Such probiotic supplementation	1319:1348	Such probiotic supplementation	1319:1348	Such probiotic supplementation also resulted in pronounced changes in the composition of the gut microbiota.
35060590	3	59	dep	sulfate-induced	647:661	arg1	UC					683:684	UC	683:684	UC	683:684	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	3	59	dep	sulfate-induced	647:661	arg1	colitis					674:680	ulcerative colitis	663:680	ulcerative colitis (UC)	663:685	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	1	60	from	consequence	250:260	arg1	microenvironment					291:306	the intestinal microenvironment	276:306	the intestinal microenvironment	276:306	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	1	60	from	consequence	250:260	arg1	dysbiosis					323:331	consequent dysbiosis	312:331	consequent dysbiosis	312:331	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	8	61	theme	barrier-related	1505:1519	arg1	Claudin-1					1531:1539	Claudin-1	1531:1539	Claudin-1	1531:1539	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	61	theme	barrier-related	1505:1519	arg1	proteins					1521:1528	intestinal barrier-related proteins	1494:1528	intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin)	1494:1566	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	61	theme	barrier-related	1505:1519	arg1	ZO-1					1548:1551	ZO-1	1548:1551	ZO-1	1548:1551	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	61	theme	barrier-related	1505:1519	arg1	Occludin					1558:1565	Occludin	1558:1565	Occludin	1558:1565	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	8	61	theme	barrier-related	1505:1519	arg1	MUC2					1542:1545	MUC2	1542:1545	MUC2	1542:1545	Moreover, BL-99 intervention markedly increased the expression of intestinal barrier-related proteins (Claudin-1, MUC2, ZO-1, and Occludin).
35060590	3	62	theme	sodium	640:645	arg1	model					623:627	an experimental model	607:627	an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC)	607:685	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	3	63	theme	lactis	547:552	arg1	administration					560:573	Bifidobacterium lactis BL-99 administration	531:573	Bifidobacterium lactis BL-99 administration	531:573	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	4	64	theme	activity	897:904	arg1	scores					912:917	disease activity index scores	889:917	disease activity index scores	889:917	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	65	theme	proinflammatory	942:956	arg1	IL-1β					976:980	IL-1β	976:980	IL-1β	976:980	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	65	theme	proinflammatory	942:956	arg1	IL-6					983:986	IL-6	983:986	IL-6	983:986	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	65	theme	proinflammatory	942:956	arg1	<					1003:1003	P < 0.05	1001:1008	P < 0.05	1001:1008	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	65	theme	proinflammatory	942:956	arg1	cytokines					958:966	proinflammatory cytokines	942:966	proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05)	942:1009	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	65	theme	proinflammatory	942:956	arg1	TNF-α					969:973	TNF-α	969:973	TNF-α	969:973	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	65	theme	proinflammatory	942:956	arg1	IL-17					993:997	IL-17	993:997	IL-17	993:997	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	5	66	theme	BL-99-mediated	1066:1079	arg1	reductions					1081:1090	BL-99-mediated reductions	1066:1090	BL-99-mediated reductions in tissue injury severity	1066:1116	Colon tissue pathological sections similarly revealed BL-99-mediated reductions in tissue injury severity.
35060590	3	67	theme	sulfate-induced	647:661	arg1	sodium					640:645	dextran sodium	632:645	dextran sodium sulfate-induced ulcerative colitis (UC)	632:685	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	5	68	theme	tissue	1018:1023	arg1	sections					1038:1045	Colon tissue pathological sections	1012:1045	Colon tissue pathological sections	1012:1045	Colon tissue pathological sections similarly revealed BL-99-mediated reductions in tissue injury severity.
35060590	9	69	theme	intestinal	1756:1765	arg1	microflora					1767:1776	the intestinal microflora	1752:1776	the intestinal microflora	1752:1776	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	9	70	theme	probiotic	1644:1652	arg1	preparation					1654:1664	a beneficial probiotic preparation	1631:1664	a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production	1631:1825	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	9	70	theme	probiotic	1644:1652	arg1	BL-99					1606:1610	BL-99	1606:1610	BL-99	1606:1610	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	6	71	theme	trabecular	1250:1259	arg1	<					1310:1310	P < 0.05	1308:1315	P < 0.05	1308:1315	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	6	71	theme	trabecular	1250:1259	arg1	number					1261:1266	trabecular number	1250:1266	trabecular number	1250:1266	Micro-computed tomography (Micro-CT) analyses further exhibited significant improvements in percent bone volume (BV/TV) as well as trabecular number and thickness in BL-99-treated animals (P < 0.05).
35060590	2	72	theme	probiotic	373:381	arg1	treatment					383:391	anti-inflammatory probiotic treatment	355:391	anti-inflammatory probiotic treatment	355:391	We hypothesized that anti-inflammatory probiotic treatment would be sufficient to alleviate intestinal inflammation and thereby prevent the development of osteoporosis.
35060590	4	73	theme	BL-99	814:818	arg1	administration					820:833	BL-99 administration	814:833	BL-99 administration	814:833	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	3	74	theme	underlying	709:718	arg1	mechanisms					730:739	the underlying molecular mechanisms	705:739	the underlying molecular mechanisms	705:739	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	2	75	theme	anti-inflammatory	355:371	arg1	treatment					383:391	anti-inflammatory probiotic treatment	355:391	anti-inflammatory probiotic treatment	355:391	We hypothesized that anti-inflammatory probiotic treatment would be sufficient to alleviate intestinal inflammation and thereby prevent the development of osteoporosis.
35060590	4	76	theme	P	1001:1001	arg1	<					1003:1003	P < 0.05	1001:1008	P < 0.05	1001:1008	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	76	theme	P	1001:1001	arg1	cytokines					958:966	proinflammatory cytokines	942:966	proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05)	942:1009	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	3	77	theme	molecular	720:728	arg1	mechanisms					730:739	the underlying molecular mechanisms	705:739	the underlying molecular mechanisms	705:739	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	5	78	theme	Colon	1012:1016	arg1	sections					1038:1045	Colon tissue pathological sections	1012:1045	Colon tissue pathological sections	1012:1045	Colon tissue pathological sections similarly revealed BL-99-mediated reductions in tissue injury severity.
35060590	3	79	theme	Bifidobacterium	531:545	arg1	administration					560:573	Bifidobacterium lactis BL-99 administration	531:573	Bifidobacterium lactis BL-99 administration	531:573	To that end, the ability of Bifidobacterium lactis BL-99 administration to protect against bone loss in an experimental model of dextran sodium sulfate-induced ulcerative colitis (UC) was analyzed, and the underlying molecular mechanisms were interrogated in detail.
35060590	4	80	theme	P	878:878	arg1	loss					872:875	colitis-associated weight loss	846:875	colitis-associated weight loss (P < 0.05)	846:886	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	4	80	theme	P	878:878	arg1	<					880:880	P < 0.05	878:885	P < 0.05	878:885	The results of these analyses revealed that BL-99 administration suppressed colitis-associated weight loss (P < 0.05), disease activity index scores, and the production of proinflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17) (P < 0.05).
35060590	1	81	theme	inflammatory	155:166	arg1	disease					174:180	inflammatory bowel disease	155:180	inflammatory bowel disease	155:180	Patients diagnosed with inflammatory bowel disease or related conditions also frequently suffer from osteoporosis as a consequence of changes in the intestinal microenvironment and consequent dysbiosis.
35060590	9	82	theme	beneficial	1633:1642	arg1	preparation					1654:1664	a beneficial probiotic preparation	1631:1664	a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production	1631:1825	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35060590	9	82	theme	beneficial	1633:1642	arg1	BL-99					1606:1610	BL-99	1606:1610	BL-99	1606:1610	Together, these results suggest that BL-99 can be utilized as a beneficial probiotic preparation to prevent the incidence of osteoporosis in UC patients owing to its ability to shape the intestinal microflora and to suppress inflammatory cytokine production.
35482194	4	0	theme	ECM	652:654	arg1	biopanning					656:665	ECM biopanning	652:665	ECM biopanning	652:665	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	2	1	dep	glycosylated	260:271	arg1	secreted					274:281	secreted	274:281	secreted	274:281	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	0	2	theme	Surface	78:84	arg1	Display					86:92	Yeast Surface Display	72:92	Yeast Surface Display	72:92	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	1	3	theme	proteins	143:150	arg1	mixture					132:138	a rich mixture	125:138	a rich mixture of proteins and glycans secreted by cells	125:180	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	1	3	theme	proteins	143:150	arg1	matrix					109:114	Extracellular matrix	95:114	Extracellular matrix (ECM)	95:120	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	4	4	theme	receptor	551:558	arg1	library					588:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	3	5	theme	Certain	331:337	arg1	components					339:348	Certain components	331:348	Certain components of ECM	331:355	Certain components of ECM are ubiquitous among all tissue; however, each biological tissue also displays unique variations that can be identified using biopanning techniques.
35482194	6	6	dep	VLRs	989:992	arg1	using					994:998	using	994:998	using yeast surface display techniques	994:1031	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	6	6	dep	VLRs	989:992	arg1	adapted					1053:1059	adapted	1053:1059	could easily be adapted for other binding scaffolds or ECM from other tissues	1037:1113	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	2	7	theme	glycosylated	260:271	arg1	factors					307:313	growth factors	300:313	growth factors	300:313	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	7	theme	glycosylated	260:271	arg1	collagen					228:235	collagen	228:235	collagen	228:235	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	7	theme	glycosylated	260:271	arg1	proteins					283:290	glycosylated, secreted proteins	260:290	typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases	197:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	7	theme	glycosylated	260:271	arg1	peptidases					319:328	peptidases	319:328	peptidases	319:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	7	theme	glycosylated	260:271	arg1	heparin					241:247	heparin	241:247	heparin	241:247	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	6	8	theme	ECM-binding	977:987	arg1	VLRs					989:992	tissue-selective ECM-binding VLRs	960:992	tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues	960:1113	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	2	9	gly	glycosylated	260:271	arg1	factors					307:313	growth factors	300:313	growth factors	300:313	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	9	gly	glycosylated	260:271	arg1	collagen					228:235	collagen	228:235	collagen	228:235	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	9	gly	glycosylated	260:271	arg1	proteins					283:290	glycosylated, secreted proteins	260:290	typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases	197:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	9	gly	glycosylated	260:271	arg1	peptidases					319:328	peptidases	319:328	peptidases	319:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	9	gly	glycosylated	260:271	arg1	heparin					241:247	heparin	241:247	heparin	241:247	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	5	10	attach	released	867:874	arg2	VLRs					862:865	VLRs	862:865	VLRs released from the yeast surface	862:897	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	5	10	attach	released	867:874	arg1	surface					891:897	the yeast surface	881:897	the yeast surface	881:897	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	4	11	theme	VLR	561:563	arg1	library					588:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	5	12	theme	yeast	885:889	arg1	surface					891:897	the yeast surface	881:897	the yeast surface	881:897	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	3	13	theme	unique	436:441	arg1	variations					443:452	unique variations	436:452	unique variations that can be identified using biopanning techniques	436:503	Certain components of ECM are ubiquitous among all tissue; however, each biological tissue also displays unique variations that can be identified using biopanning techniques.
35482194	4	14	theme	yeast	744:748	arg1	surface					750:756	the yeast surface	740:756	the yeast surface	740:756	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	4	15	theme	brain	629:633	arg1	ECM					635:637	brain ECM	629:637	brain ECM	629:637	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	6	16	theme	tissue-selective	960:975	arg1	VLRs					989:992	tissue-selective ECM-binding VLRs	960:992	tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues	960:1113	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	6	17	theme	display	1014:1020	arg1	techniques					1022:1031	yeast surface display techniques	1000:1031	yeast surface display techniques	1000:1031	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	1	18	theme	glycans	156:162	arg1	mixture					132:138	a rich mixture	125:138	a rich mixture of proteins and glycans secreted by cells	125:180	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	1	18	theme	glycans	156:162	arg1	matrix					109:114	Extracellular matrix	95:114	Extracellular matrix (ECM)	95:120	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	5	19	theme	potential	768:776	arg1	candidates					790:799	potential ECM-binding candidates	768:799	potential ECM-binding candidates	768:799	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	6	20	theme	binding	1071:1077	arg1	scaffolds					1079:1087	other binding scaffolds	1065:1087	other binding scaffolds	1065:1087	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	4	21	theme	clonal	705:710	arg1	VLRs					712:715	clonal VLRs	705:715	clonal VLRs isolated directly from the yeast surface	705:756	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	5	22	theme	ECM-binding	778:788	arg1	candidates					790:799	potential ECM-binding candidates	768:799	potential ECM-binding candidates	768:799	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	5	23	theme	murine	835:840	arg1	sections					848:855	immunostaining murine brain sections	820:855	immunostaining murine brain sections with VLRs released from the yeast surface	820:897	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	1	24	theme	Extracellular	95:107	arg1	mixture					132:138	a rich mixture	125:138	a rich mixture of proteins and glycans secreted by cells	125:180	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	1	24	theme	Extracellular	95:107	arg1	ECM					117:119	ECM	117:119	ECM	117:119	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	1	24	theme	Extracellular	95:107	arg1	matrix					109:114	Extracellular matrix	95:114	Extracellular matrix (ECM)	95:120	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	0	25	theme	ECM	24:26	arg1	Receptors					56:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	6	26	theme	other	1065:1069	arg1	scaffolds					1079:1087	other binding scaffolds	1065:1087	other binding scaffolds	1065:1087	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	3	27	theme	ECM	353:355	arg1	components					339:348	Certain components	331:348	Certain components of ECM	331:355	Certain components of ECM are ubiquitous among all tissue; however, each biological tissue also displays unique variations that can be identified using biopanning techniques.
35482194	4	28	theme	lymphocyte	540:549	arg1	library					588:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	1	29	theme	rich	127:130	arg1	mixture					132:138	a rich mixture	125:138	a rich mixture of proteins and glycans secreted by cells	125:180	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	1	29	theme	rich	127:130	arg1	matrix					109:114	Extracellular matrix	95:114	Extracellular matrix (ECM)	95:120	Extracellular matrix (ECM) is a rich mixture of proteins and glycans secreted by cells.
35482194	0	30	theme	Variable	36:43	arg1	Receptors					56:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	4	31	theme	variable	531:538	arg1	library					588:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	0	32	theme	Binding	28:34	arg1	Receptors					56:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	5	33	theme	brain	842:846	arg1	sections					848:855	immunostaining murine brain sections	820:855	immunostaining murine brain sections with VLRs released from the yeast surface	820:897	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	6	34	theme	VLRs	989:992	arg1	identification					942:955	the identification	938:955	the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues	938:1113	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	6	35	theme	yeast	1000:1004	arg1	display					1014:1020	yeast surface display	1000:1020	yeast surface display techniques	1000:1031	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	6	36	from	tissues	1107:1113	arg1	ECM					1092:1094	ECM	1092:1094	ECM from other tissues	1092:1113	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	6	36	from	tissues	1107:1113	arg1	scaffolds					1079:1087	other binding scaffolds	1065:1087	other binding scaffolds	1065:1087	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	4	37	theme	selective	608:616	arg1	binders					618:624	selective binders	608:624	selective binders to brain ECM	608:637	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	0	38	theme	Receptors	56:64	arg1	Identification					0:13	Identification	0:13	Identification of Brain ECM Binding Variable Lymphocyte Receptors	0:64	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	5	39	with	sections	848:855	arg1	VLRs					862:865	VLRs	862:865	VLRs released from the yeast surface	862:897	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	2	40	theme	ECM	205:207	arg1	structures					209:218	typical ECM structures	197:218	typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases	197:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	40	theme	ECM	205:207	arg1	factors					307:313	growth factors	300:313	growth factors	300:313	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	40	theme	ECM	205:207	arg1	heparin					241:247	heparin	241:247	heparin	241:247	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	40	theme	ECM	205:207	arg1	peptidases					319:328	peptidases	319:328	peptidases	319:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	40	theme	ECM	205:207	arg1	collagen					228:235	collagen	228:235	collagen	228:235	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	4	41	theme	rapid	674:678	arg1	screen					692:697	a rapid ELISA-based screen	672:697	a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface	672:756	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	0	42	theme	Lymphocyte	45:54	arg1	Receptors					56:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	2	43	theme	typical	197:203	arg1	structures					209:218	typical ECM structures	197:218	typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases	197:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	43	theme	typical	197:203	arg1	factors					307:313	growth factors	300:313	growth factors	300:313	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	43	theme	typical	197:203	arg1	heparin					241:247	heparin	241:247	heparin	241:247	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	43	theme	typical	197:203	arg1	peptidases					319:328	peptidases	319:328	peptidases	319:328	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	2	43	theme	typical	197:203	arg1	collagen					228:235	collagen	228:235	collagen	228:235	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	4	44	attach	isolated	717:724	arg1	surface					750:756	the yeast surface	740:756	the yeast surface	740:756	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	4	44	attach	isolated	717:724	arg2	VLRs					712:715	clonal VLRs	705:715	clonal VLRs isolated directly from the yeast surface	705:756	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	2	45	theme	growth	300:305	arg1	factors					307:313	growth factors	300:313	growth factors	300:313	This includes typical ECM structures such as collagen and heparin as well as glycosylated, secreted proteins such as growth factors and peptidases.
35482194	6	46	theme	other	1101:1105	arg1	tissues					1107:1113	other tissues	1101:1113	other tissues	1101:1113	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	4	47	theme	surface	572:578	arg1	library					588:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	4	48	theme	ELISA-based	680:690	arg1	screen					692:697	a rapid ELISA-based screen	672:697	a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface	672:756	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	4	49	theme	yeast	566:570	arg1	library					588:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35482194	0	50	theme	Yeast	72:76	arg1	Display					86:92	Yeast Surface Display	72:92	Yeast Surface Display	72:92	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	5	51	theme	immunostaining	820:833	arg1	sections					848:855	immunostaining murine brain sections	820:855	immunostaining murine brain sections with VLRs released from the yeast surface	820:897	Finally, potential ECM-binding candidates can be verified by immunostaining murine brain sections with VLRs released from the yeast surface.
35482194	3	52	theme	biopanning	483:492	arg1	techniques					494:503	biopanning techniques	483:503	biopanning techniques	483:503	Certain components of ECM are ubiquitous among all tissue; however, each biological tissue also displays unique variations that can be identified using biopanning techniques.
35482194	3	53	theme	biological	404:413	arg1	tissue					415:420	each biological tissue	399:420	each biological tissue	399:420	Certain components of ECM are ubiquitous among all tissue; however, each biological tissue also displays unique variations that can be identified using biopanning techniques.
35482194	6	54	theme	surface	1006:1012	arg1	display					1014:1020	yeast surface display	1000:1020	yeast surface display techniques	1000:1031	These methods provide a framework for the identification of tissue-selective ECM-binding VLRs using yeast surface display techniques and could easily be adapted for other binding scaffolds or ECM from other tissues.
35482194	0	55	theme	Brain	18:22	arg1	Receptors					56:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Brain ECM Binding Variable Lymphocyte Receptors	18:64	Identification of Brain ECM Binding Variable Lymphocyte Receptors Using Yeast Surface Display.
35482194	4	56	theme	display	580:586	arg1	library					588:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	a variable lymphocyte receptor (VLR) yeast surface display library	529:594	Here we describe using a variable lymphocyte receptor (VLR) yeast surface display library to identify selective binders to brain ECM by combining ECM biopanning with a rapid ELISA-based screen using clonal VLRs isolated directly from the yeast surface.
35768251	7	0	contain	has	1203:1205	arg1	deletion					1186:1193	deletion	1186:1193	deletion of T1R3	1186:1201	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	7	0	contain	has	1203:1205	arg2	role					1215:1218	a minor role	1207:1218	a minor role	1207:1218	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	5	1	from	expression	908:917	arg1	colon					922:926	colon	922:926	colon	922:926	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	6	2	theme	Other	929:933	arg1	composition					1051:1061	gut microbiota composition	1036:1061	gut microbiota composition	1036:1061	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	2	theme	Other	929:933	arg1	response					955:962	response	955:962	response to microbial stimuli in splenic lymphocytes and peritoneal macrophages	955:1033	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	2	theme	Other	929:933	arg1	expression					1068:1077	expression	1068:1077	expression of autophagy-related proteins	1068:1107	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	2	theme	Other	929:933	arg1	parameters					935:944	Other parameters	929:944	Other parameters	929:944	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	0	3	theme	Sodium-Induced	63:76	arg1	Colitis					78:84	Dextran Sulfate Sodium-Induced Colitis	47:84	Dextran Sulfate Sodium-Induced Colitis in Mice	47:92	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	2	4	theme	type	297:300	arg1	autophagy					317:325	autophagy	317:325	autophagy	317:325	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	2	4	theme	type	297:300	arg1	imbalance					273:281	imbalance	273:281	imbalance of type 1 and type 2 immunity	273:311	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	7	5	theme	T1R3	1198:1201	arg1	deletion					1186:1193	deletion	1186:1193	deletion of T1R3	1186:1201	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	2	6	theme	colitis	450:456	arg1	progression					435:445	progression	435:445	progression	435:445	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	2	6	theme	colitis	450:456	arg1	incidence					421:429	incidence	421:429	incidence	421:429	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	0	7	from	Effect	0:5	arg1	Colitis					78:84	Dextran Sulfate Sodium-Induced Colitis	47:84	Dextran Sulfate Sodium-Induced Colitis in Mice	47:92	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	7	8	theme	intestinal	1223:1232	arg1	inflammation					1234:1245	intestinal inflammation	1223:1245	intestinal inflammation induced by DSS-induced acute colitis in mice	1223:1290	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	1	9	theme	small	219:223	arg1	intestine					225:233	small intestine	219:233	small intestine	219:233	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	3	10	from	colitis	566:572	arg1	mice					577:580	mice	577:580	mice	577:580	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	6	11	theme	autophagy-related	1082:1098	arg1	proteins					1100:1107	autophagy-related proteins	1082:1107	autophagy-related proteins	1082:1107	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	4	12	theme	colon	679:683	arg1	shrinking					685:693	colon shrinking	679:693	colon shrinking	679:693	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
35768251	3	13	theme	present	466:472	arg1	study					474:478	the present study	462:478	the present study	462:478	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	5	14	theme	junction	774:781	arg1	components					783:792	tight junction components	768:792	tight junction components	768:792	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	5	14	theme	junction	774:781	arg1	claudin1					808:815	claudin1	808:815	claudin1	808:815	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	4	15	theme	colon	630:634	arg1	damage					636:641	colon damage	630:641	colon damage	630:641	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
35768251	5	16	with	mice	852:855	arg1	trend					862:866	trend	862:866	trend	862:866	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	4	17	theme	reduced	654:660	arg1	inflammation					662:673	reduced inflammation	654:673	reduced inflammation	654:673	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
35768251	5	18	theme	mRNA	903:906	arg1	expression					908:917	gene mRNA expression	898:917	gene mRNA expression in colon	898:926	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	7	19	theme	acute	1270:1274	arg1	colitis					1276:1282	DSS-induced acute colitis	1258:1282	DSS-induced acute colitis in mice	1258:1290	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	2	20	theme	bowel	345:349	arg1	IBD					360:362	IBD	360:362	IBD	360:362	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	2	20	theme	bowel	345:349	arg1	disease					351:357	intestinal bowel disease	334:357	intestinal bowel disease (IBD)	334:363	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	3	21	from	mice	577:580	arg1	aggravation					539:549	aggravation	539:549	aggravation of DSS-induced colitis in mice	539:580	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	2	22	theme	potential	399:407	arg1	role					409:412	a potential role	397:412	a potential role	397:412	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	4	23	theme	relative	695:702	arg1	inflammation					662:673	reduced inflammation	654:673	reduced inflammation	654:673	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
35768251	3	24	from	aggravation	539:549	arg1	mice					577:580	mice	577:580	mice	577:580	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	1	25	theme	umami	143:147	arg1	tastes					158:163	umami or sweet tastes	143:163	umami or sweet tastes	143:163	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	6	26	theme	proteins	1100:1107	arg1	composition					1051:1061	gut microbiota composition	1036:1061	gut microbiota composition	1036:1061	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	26	theme	proteins	1100:1107	arg1	response					955:962	response	955:962	response to microbial stimuli in splenic lymphocytes and peritoneal macrophages	955:1033	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	26	theme	proteins	1100:1107	arg1	expression					1068:1077	expression	1068:1077	expression of autophagy-related proteins	1068:1107	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	2	27	contain	have	392:395	arg2	role					409:412	a potential role	397:412	a potential role	397:412	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	2	27	contain	have	392:395	arg1	T1R3					387:390	T1R3	387:390	T1R3	387:390	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	2	28	dep	type	286:289	arg1	immunity					304:311	immunity	304:311	immunity	304:311	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	6	29	theme	peritoneal	1012:1021	arg1	macrophages					1023:1033	peritoneal macrophages	1012:1033	peritoneal macrophages	1012:1033	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	30	theme	splenic	988:994	arg1	lymphocytes					996:1006	splenic lymphocytes	988:1006	splenic lymphocytes	988:1006	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	5	31	theme	components	783:792	arg1	expression					754:763	mRNA expression	749:763	mRNA expression of tight junction components, particularly claudin1	749:815	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	1	32	theme	sweet	152:156	arg1	tastes					158:163	umami or sweet tastes	143:163	umami or sweet tastes	143:163	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	0	33	theme	Taste	15:19	arg1	Deletion					35:42	T1R3 Taste Receptor Gene Deletion	10:42	T1R3 Taste Receptor Gene Deletion	10:42	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	1	34	theme	muscle	239:244	arg1	cells					246:250	muscle cells	239:250	muscle cells	239:250	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	7	35	from	colitis	1276:1282	arg1	mice					1287:1290	mice	1287:1290	mice	1287:1290	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	5	36	theme	lower	871:875	arg1	inflammation					877:888	lower inflammation	871:888	lower inflammation related gene mRNA expression in colon	871:926	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	6	37	from	expression	1068:1077	arg1	lymphocytes					996:1006	splenic lymphocytes	988:1006	splenic lymphocytes	988:1006	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	37	from	expression	1068:1077	arg1	macrophages					1023:1033	peritoneal macrophages	1012:1033	peritoneal macrophages	1012:1033	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	5	38	theme	mRNA	749:752	arg1	expression					754:763	mRNA expression	749:763	mRNA expression of tight junction components, particularly claudin1	749:815	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	0	39	theme	T1R3	10:13	arg1	Deletion					35:42	T1R3 Taste Receptor Gene Deletion	10:42	T1R3 Taste Receptor Gene Deletion	10:42	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	0	40	from	Colitis	78:84	arg1	Mice					89:92	Mice	89:92	Mice	89:92	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	1	41	theme	Taste	95:99	arg1	member					117:122	Taste receptor type 1 member 3	95:124	Taste receptor type 1 member 3 (T1R3)	95:131	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	1	41	theme	Taste	95:99	arg1	T1R3					127:130	T1R3	127:130	T1R3	127:130	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	6	42	theme	microbial	967:975	arg1	stimuli					977:983	microbial stimuli	967:983	microbial stimuli	967:983	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	0	43	theme	Gene	30:33	arg1	Deletion					35:42	T1R3 Taste Receptor Gene Deletion	10:42	T1R3 Taste Receptor Gene Deletion	10:42	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	5	44	theme	T1R3-KO	844:850	arg1	mice					852:855	T1R3-KO mice	844:855	T1R3-KO mice with trend	844:866	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	4	45	theme	DSS	734:736	arg1	treatment					738:746	DSS treatment	734:746	DSS treatment	734:746	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
35768251	7	46	theme	minor	1209:1213	arg1	role					1215:1218	a minor role	1207:1218	a minor role	1207:1218	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	0	47	theme	Receptor	21:28	arg1	Deletion					35:42	T1R3 Taste Receptor Gene Deletion	10:42	T1R3 Taste Receptor Gene Deletion	10:42	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	3	48	theme	DSS-induced	554:564	arg1	colitis					566:572	DSS-induced colitis	554:572	DSS-induced colitis in mice	554:580	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	6	49	from	composition	1051:1061	arg1	lymphocytes					996:1006	splenic lymphocytes	988:1006	splenic lymphocytes	988:1006	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	49	from	composition	1051:1061	arg1	macrophages					1023:1033	peritoneal macrophages	1012:1033	peritoneal macrophages	1012:1033	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	5	50	theme	tight	768:772	arg1	components					783:792	tight junction components	768:792	tight junction components	768:792	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	5	50	theme	tight	768:772	arg1	claudin1					808:815	claudin1	808:815	claudin1	808:815	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	3	51	theme	colitis	566:572	arg1	aggravation					539:549	aggravation	539:549	aggravation of DSS-induced colitis in mice	539:580	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	5	52	theme	gene	898:901	arg1	expression					908:917	gene mRNA expression	898:917	gene mRNA expression in colon	898:926	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	1	53	theme	receptor	101:108	arg1	member					117:122	Taste receptor type 1 member 3	95:124	Taste receptor type 1 member 3 (T1R3)	95:131	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	1	53	theme	receptor	101:108	arg1	T1R3					127:130	T1R3	127:130	T1R3	127:130	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	0	54	theme	Deletion	35:42	arg1	Effect					0:5	Effect	0:5	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.	0:93	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	3	55	theme	genetic	505:511	arg1	deletion					513:520	genetic deletion	505:520	genetic deletion of T1R3	505:528	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	7	56	theme	DSS-induced	1258:1268	arg1	colitis					1276:1282	DSS-induced acute colitis	1258:1282	DSS-induced acute colitis in mice	1258:1290	Together, these results indicated that deletion of T1R3 has a minor role in intestinal inflammation induced by DSS-induced acute colitis in mice.
35768251	1	57	theme	type	110:113	arg1	member					117:122	Taste receptor type 1 member 3	95:124	Taste receptor type 1 member 3 (T1R3)	95:131	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	1	57	theme	type	110:113	arg1	T1R3					127:130	T1R3	127:130	T1R3	127:130	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	6	58	theme	WT	1131:1132	arg1	mice					1141:1144	WT and KO mice	1131:1144	WT and KO mice	1131:1144	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	2	59	dep	incidence	421:429	arg1	the					417:419	the	417:419	the	417:419	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	6	60	from	response	955:962	arg1	lymphocytes					996:1006	splenic lymphocytes	988:1006	splenic lymphocytes	988:1006	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	6	60	from	response	955:962	arg1	macrophages					1023:1033	peritoneal macrophages	1012:1033	peritoneal macrophages	1012:1033	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	2	61	theme	type	286:289	arg1	autophagy					317:325	autophagy	317:325	autophagy	317:325	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	2	61	theme	type	286:289	arg1	imbalance					273:281	imbalance	273:281	imbalance of type 1 and type 2 immunity	273:311	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	6	62	theme	microbiota	1040:1049	arg1	composition					1051:1061	gut microbiota composition	1036:1061	gut microbiota composition	1036:1061	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	1	63	theme	type	186:189	arg1	immunity					193:200	type 2 immunity	186:200	type 2 immunity	186:200	Taste receptor type 1 member 3 (T1R3) recognize umami or sweet tastes and also contributes type 2 immunity and autophagy in small intestine and muscle cells, respectively.
35768251	6	64	theme	KO	1138:1139	arg1	mice					1141:1144	WT and KO mice	1131:1144	WT and KO mice	1131:1144	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	5	65	dep	inflammation	877:888	arg1	related					890:896	related	890:896	related	890:896	mRNA expression of tight junction components, particularly claudin1 was significantly lower in T1R3-KO mice with trend to lower inflammation related gene mRNA expression in colon.
35768251	0	66	theme	Sulfate	55:61	arg1	Colitis					78:84	Dextran Sulfate Sodium-Induced Colitis	47:84	Dextran Sulfate Sodium-Induced Colitis in Mice	47:92	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	3	67	theme	T1R3	525:528	arg1	deletion					513:520	genetic deletion	505:520	genetic deletion of T1R3	505:528	In the present study, we investigated whether genetic deletion of T1R3 impacted aggravation of DSS-induced colitis in mice.
35768251	6	68	theme	gut	1036:1038	arg1	composition					1051:1061	gut microbiota composition	1036:1061	gut microbiota composition	1036:1061	Other parameters, such as response to microbial stimuli in splenic lymphocytes and peritoneal macrophages, gut microbiota composition, and expression of autophagy-related proteins, were similar between WT and KO mice.
35768251	2	69	theme	intestinal	334:343	arg1	IBD					360:362	IBD	360:362	IBD	360:362	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	2	69	theme	intestinal	334:343	arg1	disease					351:357	intestinal bowel disease	334:357	intestinal bowel disease (IBD)	334:363	Since imbalance of type 1 and type 2 immunity and autophagy affect intestinal bowel disease (IBD), we hypothesized that T1R3 have a potential role in the incidence and progression of colitis.
35768251	0	70	theme	Dextran	47:53	arg1	Sulfate					55:61	Dextran Sulfate	47:61	Dextran Sulfate Sodium-Induced Colitis in Mice	47:92	Effect of T1R3 Taste Receptor Gene Deletion on Dextran Sulfate Sodium-Induced Colitis in Mice.
35768251	4	71	theme	WT	716:717	arg1	mice					719:722	WT mice	716:722	WT mice	716:722	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
35768251	4	72	theme	T1R3-KO	597:603	arg1	mice					605:608	T1R3-KO mice	597:608	T1R3-KO mice	597:608	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
35768251	4	73	from	reduction	617:625	arg1	damage					636:641	colon damage	630:641	colon damage	630:641	We found that T1R3-KO mice showed reduction in colon damage, including reduced inflammation and colon shrinking relative to those of WT mice following DSS treatment.
36939457	3	0	theme	HFF	720:722	arg1	cells					724:728	HFF cells	720:728	HFF cells	720:728	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	4	1	theme	labels	876:881	arg1	effects					865:871	the effects	861:871	the effects of labels and cell/sub-cell heterogeneity on binding kinetics	861:933	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	1	2	theme	kinetic	165:171	arg1	studies					173:179	Cell-based kinetic studies	154:179	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins	154:236	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	5	3	theme	fixated	1110:1116	arg1	cells					1118:1122	fixated cells	1110:1122	fixated cells	1110:1122	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	4	4	with	conjunction	806:816	arg1	microscopy					849:858	surface plasmon resonance microscopy	823:858	surface plasmon resonance microscopy	823:858	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	0	5	theme	Interactions	70:81	arg1	Measurements					41:52	Kinetic Measurements	33:52	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.	0:152	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	4	6	theme	surface	823:829	arg1	resonance					839:847	surface plasmon resonance	823:847	surface plasmon resonance microscopy	823:858	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	6	7	theme	local	1263:1267	arg1	environment					1269:1279	a local environment	1261:1279	a local environment created by partially interconnected protein molecules	1261:1333	The altered kinetics is explained on the basis of a less mobile receptor confined in a local environment created by partially interconnected protein molecules.
36939457	1	8	theme	kinetic	265:271	arg1	values					273:278	kinetic values	265:278	kinetic values	265:278	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	0	9	theme	LigandTracer	92:103	arg1	Method					105:110	LigandTracer Method	92:110	LigandTracer Method	92:110	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	4	10	theme	cell/sub-cell	887:899	arg1	heterogeneity					901:913	cell/sub-cell heterogeneity	887:913	cell/sub-cell heterogeneity	887:913	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	0	11	with	Interactions	70:81	arg1	Method					105:110	LigandTracer Method	92:110	LigandTracer Method	92:110	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	0	11	with	Interactions	70:81	arg1	Plasmon					124:130	Surface Plasmon	116:130	Surface Plasmon	116:130	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	8	12	theme	kinetic	1596:1602	arg1	information					1604:1614	reliable kinetic information	1587:1614	reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation	1587:1708	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	6	13	theme	mobile	1233:1238	arg1	receptor					1240:1247	a less mobile receptor	1226:1247	a less mobile receptor confined in a local environment created by partially interconnected protein molecules	1226:1333	The altered kinetics is explained on the basis of a less mobile receptor confined in a local environment created by partially interconnected protein molecules.
36939457	7	14	theme	binding	1417:1423	arg1	reaction					1425:1432	the binding reaction	1413:1432	the binding reaction	1413:1432	We show that cell/sub-cell heterogeneity and labels on the ligands can alter the binding reaction more significantly.
36939457	4	15	from	effects	865:871	arg1	kinetics					926:933	binding kinetics	918:933	binding kinetics	918:933	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	5	16	theme	cell	985:988	arg1	constituents					990:1001	cell constituents	985:1001	cell constituents whose structures and functions are not closely dependent on cell viability	985:1076	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	0	17	dep	Method	105:110	arg1	Microscopy					142:151	Resonance Microscopy	132:151	Resonance Microscopy	132:151	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	0	17	dep	Method	105:110	arg1	the					88:90	the	88:90	the	88:90	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	7	18	from	labels	1381:1386	arg1	ligands					1395:1401	the ligands	1391:1401	the ligands	1391:1401	We show that cell/sub-cell heterogeneity and labels on the ligands can alter the binding reaction more significantly.
36939457	3	19	theme	human	738:742	arg1	receptor					762:769	the human epithelial growth receptor 2	734:771	the human epithelial growth receptor 2	734:771	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	6	20	theme	altered	1180:1186	arg1	kinetics					1188:1195	The altered kinetics	1176:1195	The altered kinetics	1176:1195	The altered kinetics is explained on the basis of a less mobile receptor confined in a local environment created by partially interconnected protein molecules.
36939457	7	21	theme	cell/sub-cell	1349:1361	arg1	heterogeneity					1363:1375	cell/sub-cell heterogeneity	1349:1375	cell/sub-cell heterogeneity	1349:1375	We show that cell/sub-cell heterogeneity and labels on the ligands can alter the binding reaction more significantly.
36939457	8	22	theme	cell	1638:1641	arg1	constituents					1643:1654	cell constituents	1638:1654	cell constituents whose structures are not changed by chemical fixation	1638:1708	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	8	23	theme	robust	1562:1567	arg1	assays					1550:1555	assays	1550:1555	assays more robust	1550:1567	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	1	24	from	measurements	304:315	arg1	different					289:297	different	289:297	different	289:297	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	1	25	theme	ligand	184:189	arg1	studies					173:179	Cell-based kinetic studies	154:179	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins	154:236	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	2	26	theme	cross-linking	486:498	arg1	reagent					500:506	a cross-linking reagent	484:506	a cross-linking reagent	484:506	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	3	27	theme	same	563:566	arg1	conditions					581:590	the same experimental conditions	559:590	the same experimental conditions for the LigandTracer method	559:618	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	6	28	theme	protein	1317:1323	arg1	molecules					1325:1333	partially interconnected protein molecules	1292:1333	partially interconnected protein molecules	1292:1333	The altered kinetics is explained on the basis of a less mobile receptor confined in a local environment created by partially interconnected protein molecules.
36939457	0	29	theme	Live	0:3	arg1	Cells					5:9	Live Cells	0:9	Live Cells	0:9	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	6	30	theme	receptor	1240:1247	arg1	basis					1217:1221	the basis	1213:1221	the basis of a less mobile receptor confined in a local environment created by partially interconnected protein molecules	1213:1333	The altered kinetics is explained on the basis of a less mobile receptor confined in a local environment created by partially interconnected protein molecules.
36939457	3	31	theme	experimental	568:579	arg1	conditions					581:590	the same experimental conditions	559:590	the same experimental conditions for the LigandTracer method	559:618	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	1	32	theme	candidate	194:202	arg1	drug					204:207	candidate drug	194:207	candidate drug	194:207	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	0	33	theme	Fixated	18:24	arg1	Cells					26:30	Fixated Cells	18:30	Fixated Cells	18:30	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	0	34	dep	Cells	5:9	arg1	Measurements					41:52	Kinetic Measurements	33:52	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.	0:152	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	7	35	from	heterogeneity	1363:1375	arg1	ligands					1395:1401	the ligands	1391:1401	the ligands	1391:1401	We show that cell/sub-cell heterogeneity and labels on the ligands can alter the binding reaction more significantly.
36939457	5	36	theme	cell	1063:1066	arg1	viability					1068:1076	cell viability	1063:1076	cell viability	1063:1076	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	3	37	with	interactions	637:648	arg1	glycans					709:715	glycans	709:715	glycans	709:715	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	1	38	theme	drug	204:207	arg1	studies					173:179	Cell-based kinetic studies	154:179	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins	154:236	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	2	39	theme	glycosylated	437:448	arg1	forms					450:454	their glycosylated forms	431:454	their glycosylated forms	431:454	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	3	40	theme	epithelial	744:753	arg1	receptor					762:769	the human epithelial growth receptor 2	734:771	the human epithelial growth receptor 2	734:771	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	0	41	theme	Surface	116:122	arg1	Plasmon					124:130	Surface Plasmon	116:130	Surface Plasmon	116:130	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	5	42	theme	ligand	1083:1088	arg1	different					1141:1149	different	1141:1149	different	1141:1149	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	5	42	theme	ligand	1083:1088	arg1	kinetics					1098:1105	the ligand binding kinetics	1079:1105	the ligand binding kinetics at fixated cells	1079:1122	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	8	43	theme	reliable	1587:1594	arg1	information					1604:1614	reliable kinetic information	1587:1614	reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation	1587:1708	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	3	44	theme	growth	755:760	arg1	receptor					762:769	the human epithelial growth receptor 2	734:771	the human epithelial growth receptor 2	734:771	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	2	45	theme	live	541:544	arg1	cells					546:550	live cells	541:550	live cells	541:550	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	3	46	theme	fluorophore-labeled	653:671	arg1	lectins					673:679	fluorophore-labeled lectins	653:679	fluorophore-labeled lectins	653:679	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	2	47	dep	proteins	418:425	arg1	e.g.					412:415	e.g.	412:415	e.g.	412:415	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	2	48	theme	membrane	389:396	arg1	constituents					398:409	membrane constituents	389:409	membrane constituents (e.g., proteins and their glycosylated forms)	389:455	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	5	49	from	that	1156:1159	arg1	different					1141:1149	different	1141:1149	different	1141:1149	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	5	49	from	that	1156:1159	arg1	kinetics					1098:1105	the ligand binding kinetics	1079:1105	the ligand binding kinetics at fixated cells	1079:1122	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	0	50	theme	Resonance	132:140	arg1	Microscopy					142:151	Resonance Microscopy	132:151	Resonance Microscopy	132:151	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	3	51	theme	LigandTracer	600:611	arg1	method					613:618	the LigandTracer method	596:618	the LigandTracer method	596:618	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	8	52	theme	fixating	1460:1467	arg1	cells					1469:1473	fixating cells	1460:1473	fixating cells	1460:1473	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	1	53	theme	membrane	220:227	arg1	proteins					229:236	membrane proteins	220:236	membrane proteins	220:236	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	1	54	theme	purified	323:330	arg1	proteins					332:339	purified proteins	323:339	purified proteins	323:339	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	4	55	theme	resonance	839:847	arg1	microscopy					849:858	surface plasmon resonance microscopy	823:858	surface plasmon resonance microscopy	823:858	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	0	56	theme	Kinetic	33:39	arg1	Measurements					41:52	Kinetic Measurements	33:52	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.	0:152	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	8	57	theme	drug	1622:1625	arg1	binding					1627:1633	drug binding	1622:1633	drug binding to cell constituents whose structures are not changed by chemical fixation	1622:1708	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	4	58	theme	heterogeneity	901:913	arg1	effects					865:871	the effects	861:871	the effects of labels and cell/sub-cell heterogeneity on binding kinetics	861:933	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	3	59	theme	antibody	685:692	arg1	molecules					694:702	antibody molecules	685:702	antibody molecules	685:702	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	4	60	theme	plasmon	831:837	arg1	resonance					839:847	surface plasmon resonance	823:847	surface plasmon resonance microscopy	823:858	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	2	61	theme	ligand	351:356	arg1	binding					358:364	ligand binding	351:364	ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent	351:506	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	3	62	theme	SKBR3	776:780	arg1	cells					782:786	SKBR3 cells	776:786	SKBR3 cells	776:786	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	6	63	theme	interconnected	1302:1315	arg1	molecules					1325:1333	partially interconnected protein molecules	1292:1333	partially interconnected protein molecules	1292:1333	The altered kinetics is explained on the basis of a less mobile receptor confined in a local environment created by partially interconnected protein molecules.
36939457	3	64	theme	molecules	694:702	arg1	interactions					637:648	the interactions	633:648	the interactions of fluorophore-labeled lectins and antibody molecules with glycans	633:715	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	2	65	dep	constituents	398:409	arg1	proteins					418:425	proteins	418:425	proteins	418:425	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	2	65	dep	constituents	398:409	arg1	forms					450:454	their glycosylated forms	431:454	their glycosylated forms	431:454	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	4	66	theme	binding	918:924	arg1	kinetics					926:933	binding kinetics	918:933	binding kinetics	918:933	In conjunction with surface plasmon resonance microscopy, the effects of labels and cell/sub-cell heterogeneity on binding kinetics were investigated.
36939457	3	67	theme	lectins	673:679	arg1	interactions					637:648	the interactions	633:648	the interactions of fluorophore-labeled lectins and antibody molecules with glycans	633:715	Under the same experimental conditions for the LigandTracer method, we measured the interactions of fluorophore-labeled lectins and antibody molecules with glycans at HFF cells and the human epithelial growth receptor 2 at SKBR3 cells, respectively.
36939457	5	68	theme	binding	1090:1096	arg1	different					1141:1149	different	1141:1149	different	1141:1149	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	5	68	theme	binding	1090:1096	arg1	kinetics					1098:1105	the ligand binding kinetics	1079:1105	the ligand binding kinetics at fixated cells	1079:1122	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	0	69	theme	Biomolecular	57:68	arg1	Interactions					70:81	Biomolecular Interactions	57:81	Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy	57:151	Live Cells versus Fixated Cells: Kinetic Measurements of Biomolecular Interactions with the LigandTracer Method and Surface Plasmon Resonance Microscopy.
36939457	8	70	theme	experimental	1495:1506	arg1	procedures					1508:1517	experimental procedures	1495:1517	experimental procedures for drug screening	1495:1536	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	2	71	gly	glycosylated	437:448	arg1	forms					450:454	their glycosylated forms	431:454	their glycosylated forms	431:454	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	2	72	theme	fixated	369:375	arg1	cells					377:381	fixated cells	369:381	fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent	369:506	However, ligand binding to fixated cells whose membrane constituents (e.g., proteins and their glycosylated forms) are partially connected by a cross-linking reagent has not been compared to that to live cells.
36939457	8	73	theme	chemical	1692:1699	arg1	fixation					1701:1708	chemical fixation	1692:1708	chemical fixation	1692:1708	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	8	74	theme	drug	1523:1526	arg1	screening					1528:1536	drug screening	1523:1536	drug screening	1523:1536	Thus, fixating cells not only simplifies experimental procedures for drug screening and renders assays more robust but also provides reliable kinetic information about drug binding to cell constituents whose structures are not changed by chemical fixation.
36939457	5	75	from	cells	1118:1122	arg1	different					1141:1149	different	1141:1149	different	1141:1149	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	5	75	from	cells	1118:1122	arg1	kinetics					1098:1105	the ligand binding kinetics	1079:1105	the ligand binding kinetics at fixated cells	1079:1122	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
36939457	1	76	theme	Cell-based	154:163	arg1	studies					173:179	Cell-based kinetic studies	154:179	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins	154:236	Cell-based kinetic studies of ligand or candidate drug binding to membrane proteins have produced affinity and kinetic values that are different from measurements using purified proteins.
36939457	5	77	theme	live	1164:1167	arg1	cells					1169:1173	live cells	1164:1173	live cells	1164:1173	Our results revealed that, for cell constituents whose structures and functions are not closely dependent on cell viability, the ligand binding kinetics at fixated cells is only slightly different from that at live cells.
35976113	8	0	theme	nanofibers	1077:1086	arg1	deposition					1052:1061	compromised deposition	1040:1061	compromised deposition of cellulosic nanofibers in bc16	1040:1094	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	6	1	theme	lipid	848:852	arg1	BC16					834:837	BC16	834:837	BC16	834:837	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	6	1	theme	lipid	848:852	arg1	remodelase					854:863	a GPI lipid remodelase	842:863	a GPI lipid remodelase	842:863	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	10	2	theme	wall	1455:1458	arg1	biomechanics					1460:1471	cell wall biomechanics	1450:1471	cell wall biomechanics	1450:1471	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	8	3	theme	cellulosic	1066:1075	arg1	nanofibers					1077:1086	cellulosic nanofibers	1066:1086	cellulosic nanofibers	1066:1086	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	6	4	theme	GPI	844:846	arg1	BC16					834:837	BC16	834:837	BC16	834:837	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	6	4	theme	GPI	844:846	arg1	remodelase					854:863	a GPI lipid remodelase	842:863	a GPI lipid remodelase	842:863	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	6	5	theme	Yeast	751:755	arg1	mutant					763:768	Yeast gup1Δ mutant	751:768	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis	751:819	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	1	6	theme	common	176:181	arg1	modification					191:202	a common protein modification	174:202	a common protein modification that targets proteins to the plasma membrane (PM)	174:252	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	1	6	theme	common	176:181	arg1	anchoring					161:169	Glycosylphosphatidylinositol (GPI) anchoring	126:169	Glycosylphosphatidylinositol (GPI) anchoring	126:169	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	0	7	theme	wall	110:113	arg1	mechanics					115:123	cell wall mechanics	105:123	cell wall mechanics	105:123	Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.
35976113	0	8	theme	cell	105:108	arg1	mechanics					115:123	cell wall mechanics	105:123	cell wall mechanics	105:123	Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.
35976113	10	9	theme	improved	1520:1527	arg1	strength					1537:1544	improved support strength	1520:1544	improved support strength	1520:1544	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	3	10	theme	GPI	492:494	arg1	tails					502:506	GPI lipid tails	492:506	GPI lipid tails	492:506	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	10	11	theme	elite	1503:1507	arg1	crops					1509:1513	elite crops	1503:1513	elite crops with improved support strength	1503:1544	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	4	12	theme	cellulose	636:644	arg1	content					646:652	cellulose content	636:652	cellulose content	636:652	The bc16 mutant exhibits fragile internodes, resulting from reduced cell wall thickness and cellulose content.
35976113	7	13	theme	GPI	886:888	arg1	structure					896:904	GPI lipid structure	886:904	GPI lipid structure	886:904	Loss of BC16 alters GPI lipid structure and disturbs the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains.
35976113	2	14	theme	lipid	279:283	arg1	tail					285:288	the GPI lipid tail	271:288	the GPI lipid tail	271:288	Knowledge about the GPI lipid tail, which guides the secretion of GPI-anchored proteins (GPI-APs), is limited in plants.
35976113	1	15	theme	protein	183:189	arg1	modification					191:202	a common protein modification	174:202	a common protein modification that targets proteins to the plasma membrane (PM)	174:252	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	1	15	theme	protein	183:189	arg1	anchoring					161:169	Glycosylphosphatidylinositol (GPI) anchoring	126:169	Glycosylphosphatidylinositol (GPI) anchoring	126:169	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	8	16	from	deposition	1052:1061	arg1	bc16					1091:1094	bc16	1091:1094	bc16	1091:1094	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	2	17	theme	GPI	275:277	arg1	tail					285:288	the GPI lipid tail	271:288	the GPI lipid tail	271:288	Knowledge about the GPI lipid tail, which guides the secretion of GPI-anchored proteins (GPI-APs), is limited in plants.
35976113	3	18	theme	Oryza	403:407	arg1	sativa					409:414	Oryza sativa	403:414	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	0	19	theme	anchor	29:34	arg1	remodeling					42:51	Glycosylphosphatidylinositol anchor lipid remodeling	0:51	Glycosylphosphatidylinositol anchor lipid remodeling	0:51	Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.
35976113	5	20	from	apparatus	740:748	arg1	located					693:699	located	693:699	located	693:699	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	7	21	theme	lipid	988:992	arg1	nanodomains					994:1004	the PM lipid nanodomains	981:1004	the PM lipid nanodomains	981:1004	Loss of BC16 alters GPI lipid structure and disturbs the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains.
35976113	7	22	theme	BC16	874:877	arg1	Loss					866:869	Loss	866:869	Loss of BC16	866:877	Loss of BC16 alters GPI lipid structure and disturbs the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains.
35976113	4	23	theme	wall	617:620	arg1	thickness					622:630	reduced cell wall thickness	604:630	reduced cell wall thickness	604:630	The bc16 mutant exhibits fragile internodes, resulting from reduced cell wall thickness and cellulose content.
35976113	0	24	theme	Glycosylphosphatidylinositol	0:27	arg1	remodeling					42:51	Glycosylphosphatidylinositol anchor lipid remodeling	0:51	Glycosylphosphatidylinositol anchor lipid remodeling	0:51	Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.
35976113	10	25	theme	GPI	1373:1375	arg1	lipids					1377:1382	GPI lipids	1373:1382	GPI lipids	1373:1382	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	10	26	theme	cell	1450:1453	arg1	biomechanics					1460:1471	cell wall biomechanics	1450:1471	cell wall biomechanics	1450:1471	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	3	27	theme	rice	397:400	arg1	O-acyltransferase					457:473	a membrane-bound O-acyltransferase	440:473	a membrane-bound O-acyltransferase (MBOAT)	440:481	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	3	27	theme	rice	397:400	arg1	CULM16					425:430	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	8	28	theme	Young	1121:1125	arg1	modulus					1129:1135	an increased Young's modulus	1108:1135	an increased Young's modulus	1108:1135	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	3	29	theme	lipid	496:500	arg1	tails					502:506	GPI lipid tails	492:506	GPI lipid tails	492:506	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	7	30	theme	lipid	890:894	arg1	structure					896:904	GPI lipid structure	886:904	GPI lipid structure	886:904	Loss of BC16 alters GPI lipid structure and disturbs the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains.
35976113	3	31	theme	BRITTLE	417:423	arg1	O-acyltransferase					457:473	a membrane-bound O-acyltransferase	440:473	a membrane-bound O-acyltransferase (MBOAT)	440:481	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	3	31	theme	BRITTLE	417:423	arg1	CULM16					425:430	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	0	32	theme	lipid	36:40	arg1	remodeling					42:51	Glycosylphosphatidylinositol anchor lipid remodeling	0:51	Glycosylphosphatidylinositol anchor lipid remodeling	0:51	Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.
35976113	9	33	theme	cell	1256:1259	arg1	surface					1261:1267	the cell surface	1252:1267	the cell surface	1252:1267	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35976113	7	34	theme	PM	985:986	arg1	nanodomains					994:1004	the PM lipid nanodomains	981:1004	the PM lipid nanodomains	981:1004	Loss of BC16 alters GPI lipid structure and disturbs the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains.
35976113	6	35	theme	composition	800:810	arg1	analysis					812:819	GPI lipid composition analysis	790:819	GPI lipid composition analysis	790:819	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	3	36	theme	membrane-bound	442:455	arg1	MBOAT					476:480	MBOAT	476:480	MBOAT	476:480	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	3	36	theme	membrane-bound	442:455	arg1	O-acyltransferase					457:473	a membrane-bound O-acyltransferase	440:473	a membrane-bound O-acyltransferase (MBOAT)	440:481	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	3	36	theme	membrane-bound	442:455	arg1	CULM16					425:430	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	4	37	theme	fragile	569:575	arg1	internodes					577:586	fragile internodes	569:586	fragile internodes	569:586	The bc16 mutant exhibits fragile internodes, resulting from reduced cell wall thickness and cellulose content.
35976113	8	38	theme	Atomic	1007:1012	arg1	microscopy					1020:1029	Atomic force microscopy	1007:1029	Atomic force microscopy	1007:1029	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	6	39	theme	lipid	794:798	arg1	analysis					812:819	GPI lipid composition analysis	790:819	GPI lipid composition analysis	790:819	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	4	40	theme	cell	612:615	arg1	thickness					622:630	reduced cell wall thickness	604:630	reduced cell wall thickness	604:630	The bc16 mutant exhibits fragile internodes, resulting from reduced cell wall thickness and cellulose content.
35976113	3	41	theme	sativa	409:414	arg1	O-acyltransferase					457:473	a membrane-bound O-acyltransferase	440:473	a membrane-bound O-acyltransferase (MBOAT)	440:481	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	3	41	theme	sativa	409:414	arg1	CULM16					425:430	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	rice (Oryza sativa) BRITTLE CULM16 (BC16)	397:437	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	4	42	theme	reduced	604:610	arg1	thickness					622:630	reduced cell wall thickness	604:630	reduced cell wall thickness	604:630	The bc16 mutant exhibits fragile internodes, resulting from reduced cell wall thickness and cellulose content.
35976113	5	43	theme	endoplasmic	708:718	arg1	reticulum					720:728	the endoplasmic reticulum	704:728	the endoplasmic reticulum	704:728	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	6	44	theme	GPI	790:792	arg1	analysis					812:819	GPI lipid composition analysis	790:819	GPI lipid composition analysis	790:819	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	1	45	theme	plasma	233:238	arg1	PM					250:251	PM	250:251	PM	250:251	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	1	45	theme	plasma	233:238	arg1	membrane					240:247	the plasma membrane	229:247	the plasma membrane (PM)	229:252	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	8	46	theme	abnormal	1141:1148	arg1	properties					1161:1170	abnormal mechanical properties	1141:1170	abnormal mechanical properties	1141:1170	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	5	47	theme	only	667:670	arg1	BC16					655:658	BC16	655:658	BC16	655:658	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	5	47	theme	only	667:670	arg1	MBOAT					672:676	the only MBOAT	663:676	the only MBOAT in rice	663:684	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	7	48	theme	cellulose	954:962	arg1	biosynthesis					964:975	cellulose biosynthesis	954:975	cellulose biosynthesis	954:975	Loss of BC16 alters GPI lipid structure and disturbs the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains.
35976113	5	49	from	MBOAT	672:676	arg1	rice					681:684	rice	681:684	rice	681:684	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	3	50	theme	cell	520:523	arg1	biomechanics					530:541	cell wall biomechanics	520:541	cell wall biomechanics	520:541	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	1	51	theme	Glycosylphosphatidylinositol	126:153	arg1	modification					191:202	a common protein modification	174:202	a common protein modification that targets proteins to the plasma membrane (PM)	174:252	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	1	51	theme	Glycosylphosphatidylinositol	126:153	arg1	anchoring					161:169	Glycosylphosphatidylinositol (GPI) anchoring	126:169	Glycosylphosphatidylinositol (GPI) anchoring	126:169	Glycosylphosphatidylinositol (GPI) anchoring is a common protein modification that targets proteins to the plasma membrane (PM).
35976113	9	52	theme	multiple	1280:1287	arg1	organization					1320:1331	cellulose organization	1310:1331	cellulose organization	1310:1331	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35976113	9	52	theme	multiple	1280:1287	arg1	functions					1289:1297	fulfill multiple functions	1272:1297	fulfill multiple functions	1272:1297	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35976113	8	53	theme	force	1014:1018	arg1	microscopy					1020:1029	Atomic force microscopy	1007:1029	Atomic force microscopy	1007:1029	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	5	54	from	located	693:699	arg1	reticulum					720:728	the endoplasmic reticulum	704:728	the endoplasmic reticulum	704:728	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	5	54	from	located	693:699	arg1	apparatus					740:748	Golgi apparatus	734:748	Golgi apparatus	734:748	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	3	55	theme	wall	525:528	arg1	biomechanics					530:541	cell wall biomechanics	520:541	cell wall biomechanics	520:541	Here, we report that rice (Oryza sativa) BRITTLE CULM16 (BC16), a membrane-bound O-acyltransferase (MBOAT) remodels GPI lipid tails and governs cell wall biomechanics.
35976113	10	56	with	crops	1509:1513	arg1	strength					1537:1544	improved support strength	1520:1544	improved support strength	1520:1544	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	2	57	theme	proteins	334:341	arg1	secretion					308:316	the secretion	304:316	the secretion of GPI-anchored proteins (GPI-APs)	304:351	Knowledge about the GPI lipid tail, which guides the secretion of GPI-anchored proteins (GPI-APs), is limited in plants.
35976113	8	58	theme	compromised	1040:1050	arg1	deposition					1052:1061	compromised deposition	1040:1061	compromised deposition of cellulosic nanofibers in bc16	1040:1094	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	9	59	theme	BC16-mediated	1184:1196	arg1	remodeling					1204:1213	BC16-mediated lipid remodeling	1184:1213	BC16-mediated lipid remodeling	1184:1213	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35976113	5	60	located	located	693:699	arg1	reticulum					720:728	the endoplasmic reticulum	704:728	the endoplasmic reticulum	704:728	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	5	60	located	located	693:699	arg1	apparatus					740:748	Golgi apparatus	734:748	Golgi apparatus	734:748	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	5	60	located	located	693:699	arg2	BC16					655:658	BC16	655:658	BC16	655:658	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	5	60	located	located	693:699	arg2	MBOAT					672:676	the only MBOAT	663:676	the only MBOAT in rice	663:684	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	2	61	theme	GPI-anchored	321:332	arg1	proteins					334:341	GPI-anchored proteins	321:341	GPI-anchored proteins (GPI-APs)	321:351	Knowledge about the GPI lipid tail, which guides the secretion of GPI-anchored proteins (GPI-APs), is limited in plants.
35976113	2	61	theme	GPI-anchored	321:332	arg1	GPI-APs					344:350	GPI-APs	344:350	GPI-APs	344:350	Knowledge about the GPI lipid tail, which guides the secretion of GPI-anchored proteins (GPI-APs), is limited in plants.
35976113	4	62	theme	bc16	548:551	arg1	mutant					553:558	The bc16 mutant	544:558	The bc16 mutant	544:558	The bc16 mutant exhibits fragile internodes, resulting from reduced cell wall thickness and cellulose content.
35976113	7	63	theme	BC1	936:938	arg1	targeting					923:931	the targeting	919:931	the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains	919:1004	Loss of BC16 alters GPI lipid structure and disturbs the targeting of BC1, a GPI-AP for cellulose biosynthesis, to the PM lipid nanodomains.
35976113	9	64	theme	lipid	1198:1202	arg1	remodeling					1204:1213	BC16-mediated lipid remodeling	1184:1213	BC16-mediated lipid remodeling	1184:1213	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35976113	10	65	theme	biomechanics	1460:1471	arg1	control					1439:1445	the control	1435:1445	the control of cell wall biomechanics	1435:1471	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	6	66	theme	gup1Δ	757:761	arg1	mutant					763:768	Yeast gup1Δ mutant	751:768	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis	751:819	Yeast gup1Δ mutant restoring assay and GPI lipid composition analysis demonstrated BC16 as a GPI lipid remodelase.
35976113	8	67	theme	mechanical	1150:1159	arg1	properties					1161:1170	abnormal mechanical properties	1141:1170	abnormal mechanical properties	1141:1170	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	9	68	theme	cellulose	1310:1318	arg1	organization					1320:1331	cellulose organization	1310:1331	cellulose organization	1310:1331	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35976113	0	69	theme	plasma	77:82	arg1	membrane					84:91	the plasma membrane	73:91	the plasma membrane	73:91	Glycosylphosphatidylinositol anchor lipid remodeling directs proteins to the plasma membrane and governs cell wall mechanics.
35976113	5	70	from	reticulum	720:728	arg1	located					693:699	located	693:699	located	693:699	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	8	71	theme	increased	1111:1119	arg1	modulus					1129:1135	an increased Young's modulus	1108:1135	an increased Young's modulus	1108:1135	Atomic force microscopy revealed compromised deposition of cellulosic nanofibers in bc16, leading to an increased Young's modulus and abnormal mechanical properties.
35976113	5	72	theme	Golgi	734:738	arg1	apparatus					740:748	Golgi apparatus	734:748	Golgi apparatus	734:748	BC16 is the only MBOAT in rice and is located in the endoplasmic reticulum and Golgi apparatus.
35976113	10	73	theme	support	1529:1535	arg1	strength					1537:1544	improved support strength	1520:1544	improved support strength	1520:1544	Our work unravels a mechanism by which GPI lipids are remodeled in plants and provides insights into the control of cell wall biomechanics, offering a tool for breeding elite crops with improved support strength.
35976113	9	74	theme	fulfill	1272:1278	arg1	organization					1320:1331	cellulose organization	1310:1331	cellulose organization	1310:1331	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35976113	9	74	theme	fulfill	1272:1278	arg1	functions					1289:1297	fulfill multiple functions	1272:1297	fulfill multiple functions	1272:1297	Therefore, BC16-mediated lipid remodeling directs the GPI-APs, such as BC1, to the cell surface to fulfill multiple functions, including cellulose organization.
35817248	9	0	theme	only	1517:1520	arg1	load					1528:1531	only lipid load	1517:1531	only lipid load	1517:1531	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	9	0	theme	only	1517:1520	arg1	group					1510:1514	negative control group	1493:1514	negative control group (only lipid load)	1493:1532	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	3	1	theme	antioxidant	486:496	arg1	activity					498:505	The antioxidant activity	482:505	The antioxidant activity	482:505	The antioxidant activity also was investigated.
35817248	4	2	theme	lipase	583:588	arg1	action					573:578	MATERIALS AND METHODS Enzymatic inhibitory action	530:578	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase	530:618	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase was evaluated in vitro.
35817248	5	3	theme	lipid	649:653	arg1	OSTT					690:693	OSTT	690:693	OSTT	690:693	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	3	theme	lipid	649:653	arg1	OLTT					656:659	Oral lipid (OLTT) and starch tolerance tests	644:687	OLTT	656:659	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	14	4	theme	scavenging	2207:2216	arg1	radicals					2223:2230	scavenging free radicals	2207:2230	scavenging free radicals	2207:2230	Both extracts inhibit lipid peroxidation and scavenging free radicals in vitro.
35817248	10	5	theme	significant	1572:1582	arg1	decrease					1584:1591	a significant decrease	1570:1591	a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A)	1570:1633	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	12	6	theme	main	1945:1948	arg1	acid					2001:2004	coumaric acid	1992:2004	coumaric acid	1992:2004	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	12	6	theme	main	1945:1948	arg1	composition					1959:1969	The main chemical composition	1941:1969	The main chemical composition of both extracts	1941:1986	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	8	7	theme	α-amylase	1157:1165	arg1	IC50					1149:1152	The IC50	1145:1152	The IC50 of α-amylase	1145:1165	The IC50 of α-amylase was higher (41.8 μg/μL for O-E and 25.2 μg/μL for O-A) than the Acarbose (2.5 μg/μL); while α-glucosidase results showed not statistically differences between groups (∼1.7 μg/μL).
35817248	8	7	theme	α-amylase	1157:1165	arg1	higher					1171:1176	higher	1171:1176	higher	1171:1176	The IC50 of α-amylase was higher (41.8 μg/μL for O-E and 25.2 μg/μL for O-A) than the Acarbose (2.5 μg/μL); while α-glucosidase results showed not statistically differences between groups (∼1.7 μg/μL).
35817248	5	8	theme	tolerance	673:681	arg1	tests					683:687	Oral lipid (OLTT) and starch tolerance tests	644:687	tests	683:687	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	7	9	theme	inhibitory	994:1003	arg1	IC50					1027:1030	IC50	1027:1030	IC50	1027:1030	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	7	9	theme	inhibitory	994:1003	arg1	concentration					1012:1024	inhibitory median concentration	994:1024	higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A)	987:1092	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	10	10	theme	negative	1644:1651	arg1	load					1680:1683	only starch load	1668:1683	only starch load	1668:1683	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	10	10	theme	negative	1644:1651	arg1	group					1661:1665	the negative control group	1640:1665	the negative control group (only starch load)	1640:1684	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	5	11	theme	102 mg/kg	775:783	arg1	weight					790:795	102 mg/kg body weight	775:795	102 mg/kg body weight	775:795	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	7	12	theme	lipase	1036:1041	arg1	activity					1043:1050	lipase activity	1036:1050	lipase activity	1036:1050	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	11	13	theme	scavenged	1821:1829	arg1	∼2.0 μg/μL					1846:1855	∼2.0 μg/μL	1846:1855	∼2.0 μg/μL	1846:1855	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	11	13	theme	scavenged	1821:1829	arg1	radicals					1836:1843	scavenged free radicals	1821:1843	scavenged free radicals (∼2.0 μg/μL)	1821:1856	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	5	14	theme	Wistar	838:843	arg1	rats					845:848	male Wistar rats	833:848	male Wistar rats	833:848	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	6	15	theme	radical	946:952	arg1	inhibition					898:907	inhibition	898:907	inhibition of lipid peroxidation and scavenging radical	898:952	The antioxidant activity was evaluated through inhibition of lipid peroxidation and scavenging radical.
35817248	5	16	theme	L.	716:717	arg1	O-A					739:741	O-A	739:741	O-A	739:741	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	16	theme	L.	716:717	arg1	acetone					730:736	L. graveolens acetone	716:736	L. graveolens acetone (O-A)	716:742	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	17	theme	acetone	730:736	arg1	extracts					762:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts	716:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses)	716:828	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	17	theme	acetone	730:736	arg1	doses					823:827	equivalent to a 1 g human doses	797:827	equivalent to a 1 g human doses	797:827	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	6	18	theme	lipid	912:916	arg1	peroxidation					918:929	lipid peroxidation	912:929	lipid peroxidation	912:929	The antioxidant activity was evaluated through inhibition of lipid peroxidation and scavenging radical.
35817248	0	19	theme	digestive	75:83	arg1	enzymes					85:91	digestive enzymes	75:91	digestive enzymes	75:91	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth) extracts on digestive enzymes in vitro, and beneficial impact on carbohydrates and lipids absorption in vivo.
35817248	7	20	theme	positive	1103:1110	arg1	control					1112:1118	the positive control	1099:1118	the positive control (Orlistat) (0.07 μg/μL)	1099:1142	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	7	20	theme	positive	1103:1110	arg1	Orlistat					1121:1128	Orlistat	1121:1128	Orlistat	1121:1128	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	7	20	theme	positive	1103:1110	arg1	0.07 μg/μL					1132:1141	0.07 μg/μL	1132:1141	0.07 μg/μL	1132:1141	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	2	21	theme	STUDY	365:369	arg1	AIM					354:356	AIM	354:356	AIM OF THE STUDY	354:369	AIM OF THE STUDY Assess the effect of Mexican oregano (Lippia graveolens Kunth) on carbohydrates and lipids absorption in vivo.
35817248	0	22	theme	Inhibitory	0:9	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth)	0:61	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth) extracts on digestive enzymes in vitro, and beneficial impact on carbohydrates and lipids absorption in vivo.
35817248	4	23	theme	MATERIALS	530:538	arg1	action					573:578	MATERIALS AND METHODS Enzymatic inhibitory action	530:578	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase	530:618	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase was evaluated in vitro.
35817248	13	24	dep	CONCLUSIONS	2056:2066	arg1	Both					2068:2071	Both	2068:2071	Both	2068:2071	CONCLUSIONS Both extracts reduce lipid absorption; whereas O-E decreases carbohydrate absorption in vivo.
35817248	2	25	theme	oregano	400:406	arg1	effect					382:387	the effect	378:387	the effect of Mexican oregano (Lippia graveolens Kunth) on carbohydrates and lipids absorption	378:471	AIM OF THE STUDY Assess the effect of Mexican oregano (Lippia graveolens Kunth) on carbohydrates and lipids absorption in vivo.
35817248	4	26	theme	METHODS	544:550	arg1	action					573:578	MATERIALS AND METHODS Enzymatic inhibitory action	530:578	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase	530:618	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase was evaluated in vitro.
35817248	8	27	theme	α-glucosidase	1259:1271	arg1	results					1273:1279	α-glucosidase results	1259:1279	α-glucosidase results	1259:1279	The IC50 of α-amylase was higher (41.8 μg/μL for O-E and 25.2 μg/μL for O-A) than the Acarbose (2.5 μg/μL); while α-glucosidase results showed not statistically differences between groups (∼1.7 μg/μL).
35817248	0	28	theme	oregano	29:35	arg1	effect					11:16	Inhibitory effect	0:16	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth)	0:61	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth) extracts on digestive enzymes in vitro, and beneficial impact on carbohydrates and lipids absorption in vivo.
35817248	1	29	theme	carbohydrates	297:309	arg1	absorption					283:292	the absorption	279:292	the absorption of carbohydrates and lipids	279:320	ETHNOPHARMACOLOGICAL RELEVANCE Although Mexican oregano inhibits digestive enzymes in vitro its effect on the absorption of carbohydrates and lipids in vivo has not been addressed.
35817248	1	30	theme	lipids	315:320	arg1	absorption					283:292	the absorption	279:292	the absorption of carbohydrates and lipids	279:320	ETHNOPHARMACOLOGICAL RELEVANCE Although Mexican oregano inhibits digestive enzymes in vitro its effect on the absorption of carbohydrates and lipids in vivo has not been addressed.
35817248	5	31	dep	L.	716:717	arg1	graveolens					719:728	graveolens	719:728	graveolens	719:728	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	10	32	theme	glucose	1548:1554	arg1	levels					1556:1561	glucose levels	1548:1561	glucose levels	1548:1561	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	9	33	theme	control	1502:1508	arg1	load					1528:1531	only lipid load	1517:1531	only lipid load	1517:1531	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	9	33	theme	control	1502:1508	arg1	group					1510:1514	negative control group	1493:1514	negative control group (only lipid load)	1493:1532	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	7	34	dep	higher	987:992	arg1	concentration					1012:1024	inhibitory median concentration	994:1024	higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A)	987:1092	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	7	34	dep	higher	987:992	arg1	IC50					1027:1030	IC50	1027:1030	IC50	1027:1030	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	7	34	dep	higher	987:992	arg1	1.9 μg/μL					1053:1061	1.9 μg/μL	1053:1061	1.9 μg/μL for O-E and 1.8 μg/μL for O-A	1053:1091	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	11	35	theme	antioxidant	1702:1712	arg1	evaluation					1714:1723	in vitro antioxidant evaluation	1693:1723	in vitro antioxidant evaluation	1693:1723	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	1	36	theme	digestive	238:246	arg1	enzymes					248:254	digestive enzymes	238:254	digestive enzymes	238:254	ETHNOPHARMACOLOGICAL RELEVANCE Although Mexican oregano inhibits digestive enzymes in vitro its effect on the absorption of carbohydrates and lipids in vivo has not been addressed.
35817248	11	37	theme	in	1693:1694	arg1	evaluation					1714:1723	in vitro antioxidant evaluation	1693:1723	in vitro antioxidant evaluation	1693:1723	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	9	38	theme	lipid	1522:1526	arg1	load					1528:1531	only lipid load	1517:1531	only lipid load	1517:1531	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	9	38	theme	lipid	1522:1526	arg1	group					1510:1514	negative control group	1493:1514	negative control group (only lipid load)	1493:1532	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	4	39	theme	inhibitory	562:571	arg1	action					573:578	MATERIALS AND METHODS Enzymatic inhibitory action	530:578	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase	530:618	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase was evaluated in vitro.
35817248	7	40	dep	RESULTS	955:961	arg1	exhibited					977:985	exhibited	977:985	exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL)	977:1142	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	5	41	theme	Oral	644:647	arg1	OSTT					690:693	OSTT	690:693	OSTT	690:693	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	41	theme	Oral	644:647	arg1	OLTT					656:659	Oral lipid (OLTT) and starch tolerance tests	644:687	OLTT	656:659	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	14	42	theme	free	2218:2221	arg1	radicals					2223:2230	scavenging free radicals	2207:2230	scavenging free radicals	2207:2230	Both extracts inhibit lipid peroxidation and scavenging free radicals in vitro.
35817248	9	43	theme	serum	1412:1416	arg1	triglycerides					1418:1430	serum triglycerides	1412:1430	serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A)	1412:1474	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	11	44	theme	free	1831:1834	arg1	∼2.0 μg/μL					1846:1855	∼2.0 μg/μL	1846:1855	∼2.0 μg/μL	1846:1855	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	11	44	theme	free	1831:1834	arg1	radicals					1836:1843	scavenged free radicals	1821:1843	scavenged free radicals (∼2.0 μg/μL)	1821:1856	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	5	45	theme	body	785:788	arg1	weight					790:795	102 mg/kg body weight	775:795	102 mg/kg body weight	775:795	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	14	46	theme	lipid	2184:2188	arg1	peroxidation					2190:2201	lipid peroxidation	2184:2201	lipid peroxidation	2184:2201	Both extracts inhibit lipid peroxidation and scavenging free radicals in vitro.
35817248	5	47	theme	equivalent	797:806	arg1	extracts					762:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts	716:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses)	716:828	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	47	theme	equivalent	797:806	arg1	doses					823:827	equivalent to a 1 g human doses	797:827	equivalent to a 1 g human doses	797:827	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	12	48	theme	chemical	1950:1957	arg1	acid					2001:2004	coumaric acid	1992:2004	coumaric acid	1992:2004	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	12	48	theme	chemical	1950:1957	arg1	composition					1959:1969	The main chemical composition	1941:1969	The main chemical composition of both extracts	1941:1986	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	5	49	theme	starch	666:671	arg1	tests					683:687	Oral lipid (OLTT) and starch tolerance tests	644:687	tests	683:687	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	1	50	theme	ETHNOPHARMACOLOGICAL	173:192	arg1	RELEVANCE					194:202	ETHNOPHARMACOLOGICAL RELEVANCE	173:202	ETHNOPHARMACOLOGICAL RELEVANCE Although Mexican oregano	173:227	ETHNOPHARMACOLOGICAL RELEVANCE Although Mexican oregano inhibits digestive enzymes in vitro its effect on the absorption of carbohydrates and lipids in vivo has not been addressed.
35817248	11	51	theme	similar	1900:1906	arg1	peroxidation					1887:1898	lipid peroxidation	1881:1898	lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50)	1881:1938	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	4	52	theme	α-glucosidase	606:618	arg1	action					573:578	MATERIALS AND METHODS Enzymatic inhibitory action	530:578	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase	530:618	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase was evaluated in vitro.
35817248	10	53	theme	control	1653:1659	arg1	load					1680:1683	only starch load	1668:1683	only starch load	1668:1683	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	10	53	theme	control	1653:1659	arg1	group					1661:1665	the negative control group	1640:1665	the negative control group (only starch load)	1640:1684	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	13	54	theme	carbohydrate	2129:2140	arg1	absorption					2142:2151	carbohydrate absorption	2129:2151	carbohydrate absorption	2129:2151	CONCLUSIONS Both extracts reduce lipid absorption; whereas O-E decreases carbohydrate absorption in vivo.
35817248	6	55	theme	antioxidant	855:865	arg1	activity					867:874	The antioxidant activity	851:874	The antioxidant activity	851:874	The antioxidant activity was evaluated through inhibition of lipid peroxidation and scavenging radical.
35817248	11	56	theme	lipid	1881:1885	arg1	peroxidation					1887:1898	lipid peroxidation	1881:1898	lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50)	1881:1938	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	0	57	theme	carbohydrates	128:140	arg1	absorption					153:162	carbohydrates and lipids absorption	128:162	carbohydrates and lipids absorption	128:162	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth) extracts on digestive enzymes in vitro, and beneficial impact on carbohydrates and lipids absorption in vivo.
35817248	13	58	theme	lipid	2089:2093	arg1	absorption					2095:2104	lipid absorption	2089:2104	lipid absorption	2089:2104	CONCLUSIONS Both extracts reduce lipid absorption; whereas O-E decreases carbohydrate absorption in vivo.
35817248	7	59	theme	median	1005:1010	arg1	IC50					1027:1030	IC50	1027:1030	IC50	1027:1030	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	7	59	theme	median	1005:1010	arg1	concentration					1012:1024	inhibitory median concentration	994:1024	higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A)	987:1092	RESULTS Both extracts exhibited higher inhibitory median concentration (IC50) of lipase activity (1.9 μg/μL for O-E and 1.8 μg/μL for O-A) than the positive control (Orlistat) (0.07 μg/μL).
35817248	0	60	theme	lipids	146:151	arg1	absorption					153:162	carbohydrates and lipids absorption	128:162	carbohydrates and lipids absorption	128:162	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth) extracts on digestive enzymes in vitro, and beneficial impact on carbohydrates and lipids absorption in vivo.
35817248	10	61	theme	starch	1673:1678	arg1	load					1680:1683	only starch load	1668:1683	only starch load	1668:1683	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	10	61	theme	starch	1673:1678	arg1	group					1661:1665	the negative control group	1640:1665	the negative control group (only starch load)	1640:1684	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	5	62	theme	male	833:836	arg1	rats					845:848	male Wistar rats	833:848	male Wistar rats	833:848	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	6	63	theme	scavenging	935:944	arg1	radical					946:952	scavenging radical	935:952	scavenging radical	935:952	The antioxidant activity was evaluated through inhibition of lipid peroxidation and scavenging radical.
35817248	0	64	from	impact	118:123	arg1	absorption					153:162	carbohydrates and lipids absorption	128:162	carbohydrates and lipids absorption	128:162	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth) extracts on digestive enzymes in vitro, and beneficial impact on carbohydrates and lipids absorption in vivo.
35817248	5	65	from	weight	790:795	arg1	extracts					762:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts	716:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses)	716:828	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	65	from	weight	790:795	arg1	doses					823:827	equivalent to a 1 g human doses	797:827	equivalent to a 1 g human doses	797:827	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	66	theme	human	817:821	arg1	extracts					762:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts	716:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses)	716:828	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	66	theme	human	817:821	arg1	doses					823:827	equivalent to a 1 g human doses	797:827	equivalent to a 1 g human doses	797:827	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	6	67	theme	peroxidation	918:929	arg1	inhibition					898:907	inhibition	898:907	inhibition of lipid peroxidation and scavenging radical	898:952	The antioxidant activity was evaluated through inhibition of lipid peroxidation and scavenging radical.
35817248	2	68	theme	lipids	455:460	arg1	absorption					462:471	carbohydrates and lipids absorption	437:471	carbohydrates and lipids absorption	437:471	AIM OF THE STUDY Assess the effect of Mexican oregano (Lippia graveolens Kunth) on carbohydrates and lipids absorption in vivo.
35817248	8	69	dep	higher	1171:1176	arg1	25.2 μg/μL					1202:1211	25.2 μg/μL	1202:1211	25.2 μg/μL	1202:1211	The IC50 of α-amylase was higher (41.8 μg/μL for O-E and 25.2 μg/μL for O-A) than the Acarbose (2.5 μg/μL); while α-glucosidase results showed not statistically differences between groups (∼1.7 μg/μL).
35817248	8	69	dep	higher	1171:1176	arg1	41.8 μg/μL					1179:1188	41.8 μg/μL	1179:1188	41.8 μg/μL	1179:1188	The IC50 of α-amylase was higher (41.8 μg/μL for O-E and 25.2 μg/μL for O-A) than the Acarbose (2.5 μg/μL); while α-glucosidase results showed not statistically differences between groups (∼1.7 μg/μL).
35817248	10	70	dep	decrease	1584:1591	arg1	∼31 mg/dL					1594:1602	∼31 mg/dL	1594:1602	∼31 mg/dL for O-E and ∼17 mg/dL for O-A	1594:1632	In the OSTT, glucose levels showed a significant decrease (∼31 mg/dL for O-E and ∼17 mg/dL for O-A) than the negative control group (only starch load).
35817248	5	71	theme	ethanol	748:754	arg1	extracts					762:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts	716:769	L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses)	716:828	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	5	71	theme	ethanol	748:754	arg1	doses					823:827	equivalent to a 1 g human doses	797:827	equivalent to a 1 g human doses	797:827	Oral lipid (OLTT) and starch tolerance tests (OSTT) were conducted with L. graveolens acetone (O-A) and ethanol (O-E) extracts (at 102 mg/kg body weight equivalent to a 1 g human doses) in male Wistar rats.
35817248	11	72	dep	in	1693:1694	arg1	vitro					1696:1700	vitro	1696:1700	vitro	1696:1700	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	9	73	dep	triglycerides	1418:1430	arg1	∼147 mg/dL					1433:1442	∼147 mg/dL	1433:1442	∼147 mg/dL for O-E and ∼155 mg/dL for O-A	1433:1473	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	4	74	theme	Enzymatic	552:560	arg1	action					573:578	MATERIALS AND METHODS Enzymatic inhibitory action	530:578	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase	530:618	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase was evaluated in vitro.
35817248	11	75	theme	positive	1777:1784	arg1	Trolox					1795:1800	Trolox	1795:1800	Trolox	1795:1800	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	11	75	theme	positive	1777:1784	arg1	control					1786:1792	positive control	1777:1792	positive control (Trolox)	1777:1801	About in vitro antioxidant evaluation, not statistically differences between extracts and positive control (Trolox) were observed for scavenged free radicals (∼2.0 μg/μL); whereas O-A inhibited lipid peroxidation similar to the Trolox (∼0.8 μg/μL IC50).
35817248	9	76	theme	OLTT	1351:1354	arg1	results					1356:1362	The OLTT results	1347:1362	The OLTT results	1347:1362	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	2	77	theme	carbohydrates	437:449	arg1	absorption					462:471	carbohydrates and lipids absorption	437:471	carbohydrates and lipids absorption	437:471	AIM OF THE STUDY Assess the effect of Mexican oregano (Lippia graveolens Kunth) on carbohydrates and lipids absorption in vivo.
35817248	12	78	theme	coumaric	1992:1999	arg1	acid					2001:2004	coumaric acid	1992:2004	coumaric acid	1992:2004	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	12	78	theme	coumaric	1992:1999	arg1	composition					1959:1969	The main chemical composition	1941:1969	The main chemical composition of both extracts	1941:1986	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	0	79	theme	beneficial	107:116	arg1	impact					118:123	beneficial impact	107:123	beneficial impact	107:123	Inhibitory effect of Mexican oregano (Lippia graveolens Kunth) extracts on digestive enzymes in vitro, and beneficial impact on carbohydrates and lipids absorption in vivo.
35817248	12	80	theme	extracts	1979:1986	arg1	acid					2001:2004	coumaric acid	1992:2004	coumaric acid	1992:2004	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	12	80	theme	extracts	1979:1986	arg1	composition					1959:1969	The main chemical composition	1941:1969	The main chemical composition of both extracts	1941:1986	The main chemical composition of both extracts was coumaric acid, luteolin, rutinoside, naringenin, and carvacrol.
35817248	9	81	theme	negative	1493:1500	arg1	load					1528:1531	only lipid load	1517:1531	only lipid load	1517:1531	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	9	81	theme	negative	1493:1500	arg1	group					1510:1514	negative control group	1493:1514	negative control group (only lipid load)	1493:1532	The OLTT results showed that both extracts significantly reduced serum triglycerides (∼147 mg/dL for O-E and ∼155 mg/dL for O-A) as compared with negative control group (only lipid load).
35817248	4	82	theme	α-amylase	591:599	arg1	action					573:578	MATERIALS AND METHODS Enzymatic inhibitory action	530:578	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase	530:618	MATERIALS AND METHODS Enzymatic inhibitory action of lipase, α-amylase, and α-glucosidase was evaluated in vitro.
35817248	2	83	from	effect	382:387	arg1	absorption					462:471	carbohydrates and lipids absorption	437:471	carbohydrates and lipids absorption	437:471	AIM OF THE STUDY Assess the effect of Mexican oregano (Lippia graveolens Kunth) on carbohydrates and lipids absorption in vivo.
35817248	1	84	from	effect	269:274	arg1	absorption					283:292	the absorption	279:292	the absorption of carbohydrates and lipids	279:320	ETHNOPHARMACOLOGICAL RELEVANCE Although Mexican oregano inhibits digestive enzymes in vitro its effect on the absorption of carbohydrates and lipids in vivo has not been addressed.
35817248	13	85	dep	decreases	2119:2127	arg1	whereas					2107:2113	whereas	2107:2113	whereas	2107:2113	CONCLUSIONS Both extracts reduce lipid absorption; whereas O-E decreases carbohydrate absorption in vivo.
35163617	2	0	from	interactions	416:427	arg1	plant					436:440	the plant	432:440	the plant	432:440	Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown.
35163617	0	1	theme	Microbial	148:156	arg1	Community					158:166	Its Soil Microbial Community	139:166	Its Soil Microbial Community	139:166	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	11	2	contain	has	2132:2134	arg2	application					2142:2152	broad application	2136:2152	broad application prospects across economical plants	2136:2187	Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
35163617	11	2	contain	has	2132:2134	arg1	work					2118:2121	this work	2113:2121	this work	2113:2121	Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
35163617	2	3	theme	plant	353:357	arg1	PGPM					347:350	PGPM	347:350	PGPM (plant growth-promoting microbes)	347:384	Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown.
35163617	2	3	theme	plant	353:357	arg1	microbes					376:383	plant growth-promoting microbes	353:383	plant growth-promoting microbes	353:383	Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown.
35163617	4	4	theme	fresh	987:991	arg1	weight					993:998	root fresh weight	982:998	root fresh weight (p ≤ 0.05)	982:1009	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	4	5	theme	g/L	875:877	arg1	sucrose					879:885	1.5 g/L sucrose	871:885	1.5 g/L sucrose	871:885	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	2	6	theme	theoretical	277:287	arg1	framework					289:297	the theoretical framework	273:297	the theoretical framework	273:297	Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown.
35163617	6	7	theme	LB	1301:1302	arg1	treatment					1309:1317	the LB + LS treatment	1297:1317	treatment	1309:1317	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	5	8	dep	treatment	1050:1058	arg1	LB					1060:1061	LB	1060:1061	LB	1060:1061	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	5	9	theme	phenolic	1178:1185	arg1	compounds					1187:1195	phenolic compounds	1178:1195	phenolic compounds	1178:1195	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	3	10	theme	inoculant	544:552	arg1	concentrations					489:502	three concentrations	483:502	three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively)	483:738	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	10	11	theme	microbial	2060:2068	arg1	community					2070:2078	the microbial community	2056:2078	the microbial community for biocontrol potential	2056:2103	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	7	12	theme	fungal	1528:1533	arg1	diversity					1535:1543	fungal diversity	1528:1543	fungal diversity	1528:1543	Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp.
35163617	10	13	theme	combined	1882:1889	arg1	application					1891:1901	the combined application	1878:1901	the combined application of a bacterial inoculant and sucrose	1878:1938	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	3	14	theme	sucrose	667:673	arg1	concentrations					489:502	three concentrations	483:502	three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively)	483:738	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	6	15	theme	soil	1283:1286	arg1	enzymes					1288:1294	certain soil enzymes	1275:1294	certain soil enzymes	1275:1294	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	4	16	theme	root	982:985	arg1	weight					993:998	root fresh weight	982:998	root fresh weight (p ≤ 0.05)	982:1009	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	1	17	theme	important	193:201	arg1	L.					184:185	Rheum palmatum L.	169:185	Rheum palmatum L.	169:185	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	1	17	theme	important	193:201	arg1	herb					233:236	an important traditional Chinese medicinal herb	190:236	an important traditional Chinese medicinal herb	190:236	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	9	18	theme	redundancy	1692:1701	arg1	analysis					1703:1710	the redundancy analysis	1688:1710	the redundancy analysis	1688:1710	Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of Sphingomonas associated with change in potassium bioavailability.
35163617	1	19	theme	Chinese	215:221	arg1	L.					184:185	Rheum palmatum L.	169:185	Rheum palmatum L.	169:185	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	1	19	theme	Chinese	215:221	arg1	herb					233:236	an important traditional Chinese medicinal herb	190:236	an important traditional Chinese medicinal herb	190:236	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	0	20	theme	Soil	143:146	arg1	Community					158:166	Its Soil Microbial Community	139:166	Its Soil Microbial Community	139:166	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	6	21	theme	total	1343:1347	arg1	TK					1360:1361	TK	1360:1361	TK	1360:1361	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	6	21	theme	total	1343:1347	arg1	potassium					1349:1357	total potassium	1343:1357	total potassium (TK)	1343:1362	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	3	22	dep	inoculant	544:552	arg1	/mL					617:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	23	dep	Bacillus	512:519	arg1	amyloliquefaciens					521:537	amyloliquefaciens	521:537	amyloliquefaciens	521:537	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	1	24	from	herb	233:236	arg1	worldwide					252:260	demand worldwide	245:260	demand worldwide	245:260	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	3	25	theme	colony-forming	591:604	arg1	CFU					613:615	CFU	613:615	CFU	613:615	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	25	theme	colony-forming	591:604	arg1	units					606:610	1.0 × 107 colony-forming units	581:610	1.0 × 107 colony-forming units (CFU)	581:616	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	26	dep	R.	775:776	arg1	palmatum					778:785	R. palmatum	775:785	R. palmatum	775:785	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	27	from	co-effects	761:770	arg1	experiment					798:807	a field experiment	790:807	a field experiment	790:807	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	27	from	co-effects	761:770	arg1	R.					775:776	R.	775:776	R.	775:776	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	4	28	theme	0.15	929:932	arg1	g/L					934:936	g/L	934:936	g/L	934:936	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	11	29	dep	application	2142:2152	arg1	prospects					2154:2162	prospects	2154:2162	prospects across economical plants	2154:2187	Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
35163617	3	30	theme	×	585:585	arg1	CFU					613:615	CFU	613:615	CFU	613:615	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	30	theme	×	585:585	arg1	units					606:610	1.0 × 107 colony-forming units	581:610	1.0 × 107 colony-forming units (CFU)	581:616	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	6	31	theme	LS	1306:1307	arg1	treatment					1309:1317	the LB + LS treatment	1297:1317	treatment	1309:1317	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	3	32	dep	sucrose	667:673	arg1	g/L					694:696	0.15, 1.5, and 15 g/L	676:696	0.15, 1.5, and 15 g/L	676:696	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	32	dep	sucrose	667:673	arg1	MS					714:715	MS	714:715	MS	714:715	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	3	32	dep	sucrose	667:673	arg1	HS					722:723	HS	722:723	HS	722:723	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	9	33	theme	Sphingomonas	1774:1785	arg1	relationship					1758:1769	a positive relationship	1747:1769	a positive relationship of Sphingomonas associated with change in potassium bioavailability	1747:1837	Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of Sphingomonas associated with change in potassium bioavailability.
35163617	10	34	theme	R.	1978:1979	arg1	quality					1967:1973	quality	1967:1973	quality	1967:1973	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	10	34	theme	R.	1978:1979	arg1	growth					1956:1961	growth	1956:1961	growth	1956:1961	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	6	35	theme	rhizosphere	1459:1469	arg1	soil					1471:1474	rhizosphere soil	1459:1474	rhizosphere soil	1459:1474	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	10	36	dep	R.	1978:1979	arg1	palmatum					1981:1988	R. palmatum	1978:1988	R. palmatum	1978:1988	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	5	37	from	rhubarb	1143:1149	arg1	content					1105:1111	the relative content	1092:1111	the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds,	1092:1196	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	10	38	theme	plant	2015:2019	arg1	nutrients					2021:2029	plant nutrients	2015:2029	plant nutrients that contribute to alter the microbial community for biocontrol potential	2015:2103	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	5	39	theme	major	1116:1120	arg1	components					1129:1138	major active components	1116:1138	major active components in rhubarb	1116:1149	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	0	40	theme	Bacterial	0:8	arg1	Amendments					32:41	Bacterial Inoculant and Sucrose Amendments	0:41	Bacterial Inoculant and Sucrose Amendments	0:41	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	5	41	from	components	1129:1138	arg1	rhubarb					1143:1149	rhubarb	1143:1149	rhubarb	1143:1149	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	11	42	theme	sustainable	2239:2249	arg1	agriculture					2251:2261	sustainable agriculture	2239:2261	sustainable agriculture	2239:2261	Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
35163617	7	43	theme	pathogen	1618:1625	arg1	Fusarium					1627:1634	the common causative fungal pathogen Fusarium	1590:1634	the common causative fungal pathogen Fusarium	1590:1634	Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp.
35163617	3	44	theme	field	792:796	arg1	experiment					798:807	a field experiment	790:807	a field experiment	790:807	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	11	45	theme	agroecological	2210:2223	arg1	practices					2225:2233	agroecological practices	2210:2233	agroecological practices for sustainable agriculture	2210:2261	Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
35163617	1	46	theme	medicinal	223:231	arg1	L.					184:185	Rheum palmatum L.	169:185	Rheum palmatum L.	169:185	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	1	46	theme	medicinal	223:231	arg1	herb					233:236	an important traditional Chinese medicinal herb	190:236	an important traditional Chinese medicinal herb	190:236	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	7	47	theme	causative	1601:1609	arg1	pathogen					1618:1625	the common causative fungal pathogen	1590:1625	the common causative fungal pathogen Fusarium	1590:1634	Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp.
35163617	7	48	theme	LS	1567:1568	arg1	treatment					1570:1578	LS treatment	1567:1578	LS treatment	1567:1578	Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp.
35163617	5	49	theme	components	1129:1138	arg1	content					1105:1111	the relative content	1092:1111	the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds,	1092:1196	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	3	50	theme	Bacillus	512:519	arg1	inoculant					544:552	Bacillus amyloliquefaciens EZ99 inoculant	512:552	Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively)	512:661	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	6	51	theme	sucrose	1230:1236	arg1	addition					1238:1245	high sucrose addition	1225:1245	high sucrose addition	1225:1245	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	4	52	theme	1.5	871:873	arg1	g/L					875:877	g/L	875:877	g/L	875:877	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	2	53	theme	growth-promoting	359:374	arg1	PGPM					347:350	PGPM	347:350	PGPM (plant growth-promoting microbes)	347:384	Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown.
35163617	2	53	theme	growth-promoting	359:374	arg1	microbes					376:383	plant growth-promoting microbes	353:383	plant growth-promoting microbes	353:383	Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown.
35163617	1	54	theme	demand	245:250	arg1	worldwide					252:260	demand worldwide	245:260	demand worldwide	245:260	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	3	55	theme	EZ99	539:542	arg1	inoculant					544:552	Bacillus amyloliquefaciens EZ99 inoculant	512:552	Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively)	512:661	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	6	56	theme	enzymes	1288:1294	arg1	activities					1261:1270	the activities	1257:1270	the activities of certain soil enzymes	1257:1294	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	10	57	theme	sucrose	1932:1938	arg1	application					1891:1901	the combined application	1878:1901	the combined application of a bacterial inoculant and sucrose	1878:1938	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	8	58	theme	effective	1651:1659	arg1	suppression					1661:1671	an effective suppression	1648:1671	an effective suppression	1648:1671	showed an effective suppression.
35163617	3	59	theme	×	559:559	arg1	/mL					617:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	6	60	theme	certain	1275:1281	arg1	enzymes					1288:1294	certain soil enzymes	1275:1294	certain soil enzymes	1275:1294	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	6	61	theme	potassium	1434:1442	arg1	utilization					1444:1454	the potassium utilization	1430:1454	the potassium utilization	1430:1454	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	10	62	theme	inoculant	1918:1926	arg1	application					1891:1901	the combined application	1878:1901	the combined application of a bacterial inoculant and sucrose	1878:1938	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	1	63	theme	traditional	203:213	arg1	L.					184:185	Rheum palmatum L.	169:185	Rheum palmatum L.	169:185	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	1	63	theme	traditional	203:213	arg1	herb					233:236	an important traditional Chinese medicinal herb	190:236	an important traditional Chinese medicinal herb	190:236	Rheum palmatum L. is an important traditional Chinese medicinal herb now in demand worldwide.
35163617	10	64	theme	biocontrol	2084:2093	arg1	potential					2095:2103	biocontrol potential	2084:2103	biocontrol potential	2084:2103	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	4	65	dep	LB	834:835	arg1	sucrose					879:885	1.5 g/L sucrose	871:885	1.5 g/L sucrose	871:885	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	4	65	dep	LB	834:835	arg1	CFU/mL					853:858	1.0 × 105 CFU/mL	843:858	1.0 × 105 CFU/mL Bacillus	843:867	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	4	65	dep	LB	834:835	arg1	treatments					947:956	treatments	947:956	treatments	947:956	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	3	66	theme	×	570:570	arg1	/mL					617:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	9	67	theme	Spearman	1716:1723	arg1	correlations					1725:1736	Spearman correlations	1716:1736	Spearman correlations	1716:1736	Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of Sphingomonas associated with change in potassium bioavailability.
35163617	7	68	theme	rhizosphere	1490:1500	arg1	microbiomes					1502:1512	rhizosphere microbiomes	1490:1512	rhizosphere microbiomes	1490:1512	Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp.
35163617	4	69	theme	g/L	934:936	arg1	sucrose					938:944	0.15 g/L sucrose	929:944	0.15 g/L sucrose	929:944	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	0	70	theme	Rheum	65:69	arg1	L.					80:81	Rheum palmatum L.	65:81	Rheum palmatum L.	65:81	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	5	71	from	content	1105:1111	arg1	rhubarb					1143:1149	rhubarb	1143:1149	rhubarb	1143:1149	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	9	72	theme	positive	1749:1756	arg1	relationship					1758:1769	a positive relationship	1747:1769	a positive relationship of Sphingomonas associated with change in potassium bioavailability	1747:1837	Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of Sphingomonas associated with change in potassium bioavailability.
35163617	5	73	theme	Metabolite	1012:1021	arg1	analysis					1023:1030	Metabolite analysis	1012:1030	Metabolite analysis	1012:1030	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	0	74	theme	L.	80:81	arg1	Growth					55:60	the Growth	51:60	the Growth of Rheum palmatum L.	51:81	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	6	75	dep	increased	1333:1341	arg1	whereas					1365:1371	whereas	1365:1371	whereas	1365:1371	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	11	76	theme	broad	2136:2140	arg1	application					2142:2152	broad application	2136:2152	broad application prospects across economical plants	2136:2187	Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
35163617	6	77	theme	available	1386:1394	arg1	AK					1407:1408	AK	1407:1408	AK	1407:1408	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	6	77	theme	available	1386:1394	arg1	potassium					1396:1404	available potassium	1386:1404	available potassium (AK)	1386:1409	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
35163617	4	78	theme	×	905:905	arg1	CFU/mL					911:916	1.0 × 105 CFU/mL	901:916	1.0 × 105 CFU/mL Bacillus	901:925	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	5	79	theme	relative	1096:1103	arg1	content					1105:1111	the relative content	1092:1111	the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds,	1092:1196	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	0	80	theme	Metabolite	103:112	arg1	Composition					114:124	Its Metabolite Composition	99:124	Its Metabolite Composition	99:124	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	10	81	theme	nutrients	2021:2029	arg1	uptake					2005:2010	uptake	2005:2010	uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential	2005:2103	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	0	82	theme	Inoculant	10:18	arg1	Amendments					32:41	Bacterial Inoculant and Sucrose Amendments	0:41	Bacterial Inoculant and Sucrose Amendments	0:41	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	4	83	dep	LB	892:893	arg1	sucrose					938:944	0.15 g/L sucrose	929:944	0.15 g/L sucrose	929:944	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	4	83	dep	LB	892:893	arg1	CFU/mL					911:916	1.0 × 105 CFU/mL	901:916	1.0 × 105 CFU/mL Bacillus	901:925	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	4	84	theme	×	847:847	arg1	CFU/mL					853:858	1.0 × 105 CFU/mL	843:858	1.0 × 105 CFU/mL Bacillus	843:867	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	9	85	theme	potassium	1813:1821	arg1	bioavailability					1823:1837	potassium bioavailability	1813:1837	potassium bioavailability	1813:1837	Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of Sphingomonas associated with change in potassium bioavailability.
35163617	0	86	theme	Sucrose	24:30	arg1	Amendments					32:41	Bacterial Inoculant and Sucrose Amendments	0:41	Bacterial Inoculant and Sucrose Amendments	0:41	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	0	87	dep	Rheum	65:69	arg1	palmatum					71:78	palmatum	71:78	palmatum	71:78	Bacterial Inoculant and Sucrose Amendments Improve the Growth of Rheum palmatum L. by Reprograming Its Metabolite Composition and Altering Its Soil Microbial Community.
35163617	10	88	theme	bacterial	1908:1916	arg1	inoculant					1918:1926	a bacterial inoculant	1906:1926	a bacterial inoculant	1906:1926	Altogether, our findings suggest that the combined application of a bacterial inoculant and sucrose can improve the growth and quality of R. palmatum, and stimulate uptake of plant nutrients that contribute to alter the microbial community for biocontrol potential.
35163617	5	89	dep	components	1129:1138	arg1	anthraquinones					1159:1172	anthraquinones	1159:1172	anthraquinones	1159:1172	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	5	89	dep	components	1129:1138	arg1	compounds					1187:1195	phenolic compounds	1178:1195	phenolic compounds	1178:1195	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	3	90	theme	units	606:610	arg1	/mL					617:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL	555:619	Here, we applied three concentrations of both Bacillus amyloliquefaciens EZ99 inoculant (1.0 × 105, 1.0 × 106, and 1.0 × 107 colony-forming units (CFU)/mL, denoted as LB, MB, and HB, respectively) and sucrose (0.15, 1.5, and 15 g/L, denoted as LS, MS, and HS, respectively) to investigate their co-effects on R. palmatum in a field experiment.
35163617	7	91	theme	fungal	1611:1616	arg1	pathogen					1618:1625	the common causative fungal pathogen	1590:1625	the common causative fungal pathogen Fusarium	1590:1634	Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp.
35163617	2	92	theme	PGPM	347:350	arg1	colonization					331:342	colonization	331:342	colonization of PGPM (plant growth-promoting microbes)	331:384	Recently, the theoretical framework suggested that sucrose triggers colonization of PGPM (plant growth-promoting microbes) in the rhizosphere, but their interactions on the plant remain largely unknown.
35163617	7	93	theme	common	1594:1599	arg1	pathogen					1618:1625	the common causative fungal pathogen	1590:1625	the common causative fungal pathogen Fusarium	1590:1634	Furthermore, rhizosphere microbiomes revealed that fungal diversity was augmented in LB + LS treatment, in which the common causative fungal pathogen Fusarium spp.
35163617	11	94	theme	economical	2171:2180	arg1	plants					2182:2187	economical plants	2171:2187	economical plants	2171:2187	Hence, this work not only has broad application prospects across economical plants, but also emphasizes agroecological practices for sustainable agriculture.
35163617	9	95	from	change	1803:1808	arg1	bioavailability					1823:1837	potassium bioavailability	1813:1837	potassium bioavailability	1813:1837	Additionally, the redundancy analysis and Spearman correlations revealed a positive relationship of Sphingomonas associated with change in potassium bioavailability.
35163617	5	96	theme	active	1122:1127	arg1	components					1129:1138	major active components	1116:1138	major active components in rhubarb	1116:1149	Metabolite analysis revealed that the treatment LB + LS significantly increased the relative content of major active components in rhubarb, namely anthraquinones and phenolic compounds, by 1.5% and 2.3%.
35163617	4	97	dep	weight	993:998	arg1	≤					1003:1003	≤	1003:1003	≤	1003:1003	The results showed that LB + MS (1.0 × 105 CFU/mL Bacillus + 1.5 g/L sucrose) and LB + LS (1.0 × 105 CFU/mL Bacillus + 0.15 g/L sucrose) treatments significantly increased root fresh weight (p ≤ 0.05).
35163617	6	98	theme	high	1225:1228	arg1	addition					1238:1245	high sucrose addition	1225:1245	high sucrose addition	1225:1245	Although high sucrose addition increased the activities of certain soil enzymes, the LB + LS treatment significantly increased total potassium (TK), whereas it decreased available potassium (AK), which facilitated the potassium utilization in rhizosphere soil.
36780316	4	0	from	expression	1007:1016	arg1	lipopolysaccharides					1021:1039	lipopolysaccharides	1021:1039	lipopolysaccharides	1021:1039	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	4	0	from	expression	1007:1016	arg1	LPS					1042:1044	LPS	1042:1044	LPS	1042:1044	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	4	0	from	expression	1007:1016	arg1	HMCs					1055:1058	-treated HMCs	1046:1058	-treated HMCs	1046:1058	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	4	0	from	expression	1007:1016	arg1	rats					1069:1072	MPGN rats	1064:1072	MPGN rats	1064:1072	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	3	1	used	used	864:867	arg2	 SPSS					849:853	 SPSS 18.0	849:858	 SPSS 18.0	849:858	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	2	from	role	534:537	arg1	Design					585:590	MPGN.Research Design	571:590	MPGN.Research Design	571:590	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	3	theme	SD	826:827	arg1	Analysis					840:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	7	4	theme	AF	1444:1445	arg1	effects					1433:1439	the protective effects	1418:1439	the protective effects of AF	1418:1445	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	7	5	theme	protective	1422:1431	arg1	effects					1433:1439	the protective effects	1418:1439	the protective effects of AF	1418:1445	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	4	6	theme	AF	1156:1157	arg1	treatment					1159:1167	AF treatment	1156:1167	AF treatment	1156:1167	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	7	7	theme	PI3K/AKT/NF-κB	1483:1496	arg1	pathway					1498:1504	PI3K/AKT/NF-κB pathway	1483:1504	PI3K/AKT/NF-κB pathway	1483:1504	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	3	8	theme	fibrosis in	682:692	arg1	vivo and					707:714	fibrosis in vitro and in vivo and	682:714	fibrosis in vitro and in vivo and	682:714	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	4	9	dep	expression	1088:1097	arg1	the					1079:1081	the	1079:1081	the	1079:1081	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	3	10	theme	Dawley	818:823	arg1	Analysis					840:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	11	theme	AF	565:566	arg1	role					534:537	the role	530:537	the role	530:537	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	11	theme	AF	565:566	arg1	mechanism					552:560	possible mechanism	543:560	possible mechanism	543:560	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	12	theme	related	731:737	arg1	Sample					764:769	the related signaling pathways.Study Sample	727:769	the related signaling pathways.Study Sample	727:769	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	4	13	theme	-treated	1046:1053	arg1	HMCs					1055:1058	-treated HMCs	1046:1058	-treated HMCs	1046:1058	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	3	14	theme	possible	543:550	arg1	mechanism					552:560	possible mechanism	543:560	possible mechanism	543:560	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	4	15	theme	inflammatory	1114:1125	arg1	cytokines					1127:1135	inflammatory cytokines	1114:1135	inflammatory cytokines	1114:1135	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	5	16	theme	LPS-treated	1186:1196	arg1	HMCs					1198:1201	LPS-treated HMCs	1186:1201	LPS-treated HMCs	1186:1201	The fibrosis of LPS-treated HMCs and MPGN rats was also reduced by AF.
36780316	6	17	theme	kidney	1308:1313	arg1	function					1315:1322	kidney function	1308:1322	kidney function	1308:1322	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	3	18	theme	signaling	739:747	arg1	Sample					764:769	the related signaling pathways.Study Sample	727:769	the related signaling pathways.Study Sample	727:769	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	7	19	theme	PI3K/AKT/NF-κB	1731:1744	arg1	signaling					1746:1754	PI3K/AKT/NF-κB signaling	1731:1754	PI3K/AKT/NF-κB signaling	1731:1754	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	5	20	dep	HMCs	1198:1201	arg1	rats					1212:1215	rats	1212:1215	rats	1212:1215	The fibrosis of LPS-treated HMCs and MPGN rats was also reduced by AF.
36780316	7	21	theme	mesangial	1666:1674	arg1	overproliferation					1681:1697	mesangial cell overproliferation	1666:1697	mesangial cell overproliferation	1666:1697	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	3	22	from	effects	613:619	arg1	vivo and					707:714	fibrosis in vitro and in vivo and	682:714	fibrosis in vitro and in vivo and	682:714	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	22	from	effects	613:619	arg1	inflammation					664:675	inflammation	664:675	inflammation	664:675	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	22	from	effects	613:619	arg1	overproliferation					645:661	mesangial cell overproliferation	630:661	mesangial cell overproliferation	630:661	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	1	23	theme	 The	104:107	arg1	type					121:124	 The most common type	104:124	 The most common type of glomerulonephritis in China	104:155	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	3	24	theme	 Our	501:504	arg1	aims					511:514	 Our work aims	501:514	 Our work aims	501:514	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	2	25	dep	Paeonia	355:361	arg1	Radix					368:372	Paeonia Alba Radix	355:372	Paeonia Alba Radix	355:372	Albiflorin (AF) is an effective composition extracted from Paeonia Alba Radix and has been administrated for various diseases.
36780316	2	26	theme	various	405:411	arg1	diseases					413:420	various diseases	405:420	various diseases	405:420	Albiflorin (AF) is an effective composition extracted from Paeonia Alba Radix and has been administrated for various diseases.
36780316	4	27	theme	ki67	1002:1005	arg1	expression					1007:1016	the ki67 expression	998:1016	the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats	998:1072	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	3	28	dep	used	864:867	arg1	analyze					872:878	analyze	872:878	was used to analyze the data.Results	860:895	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	1	29	theme	common	114:119	arg1	type					121:124	 The most common type	104:124	 The most common type of glomerulonephritis in China	104:155	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	7	30	theme	pathway	1498:1504	arg1	blocking					1471:1478	the blocking	1467:1478	the blocking of PI3K/AKT/NF-κB pathway	1467:1504	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	0	31	theme	proliferative	33:45	arg1	glomerulonephritis					47:64	mesangial proliferative glomerulonephritis	23:64	mesangial proliferative glomerulonephritis	23:64	Albiflorin ameliorates mesangial proliferative glomerulonephritis by PI3K/AKT/NF-κB pathway.
36780316	3	32	theme	human	772:776	arg1	cells					788:792	human mesangial cells	772:792	human mesangial cells (HMCs)	772:799	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	32	theme	human	772:776	arg1	HMCs					795:798	HMCs	795:798	HMCs	795:798	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	2	33	theme	effective	318:326	arg1	composition					328:338	an effective composition	315:338	an effective composition extracted from Paeonia Alba Radix	315:372	Albiflorin (AF) is an effective composition extracted from Paeonia Alba Radix and has been administrated for various diseases.
36780316	2	33	theme	effective	318:326	arg1	Albiflorin					296:305	Albiflorin	296:305	Albiflorin (AF)	296:310	Albiflorin (AF) is an effective composition extracted from Paeonia Alba Radix and has been administrated for various diseases.
36780316	7	34	theme	AF	1537:1538	arg1	effects					1526:1532	the inhibitory effects	1511:1532	the inhibitory effects of AF on MPGN	1511:1546	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	1	35	theme	mesangial	224:232	arg1	overproliferation					239:255	mesangial cell overproliferation	224:255	mesangial cell overproliferation	224:255	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	0	36	theme	mesangial	23:31	arg1	glomerulonephritis					47:64	mesangial proliferative glomerulonephritis	23:64	mesangial proliferative glomerulonephritis	23:64	Albiflorin ameliorates mesangial proliferative glomerulonephritis by PI3K/AKT/NF-κB pathway.
36780316	3	37	theme	pathways.Study	749:762	arg1	Sample					764:769	the related signaling pathways.Study Sample	727:769	the related signaling pathways.Study Sample	727:769	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	6	38	theme	pathological	1356:1367	arg1	injury					1369:1374	pathological injury	1356:1374	pathological injury	1356:1374	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	3	39	theme	mesangial	630:638	arg1	overproliferation					645:661	mesangial cell overproliferation	630:661	mesangial cell overproliferation	630:661	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	4	40	theme	mRNA	1083:1086	arg1	expression					1088:1097	mRNA expression	1083:1097	mRNA expression	1083:1097	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	1	41	theme	cell	234:237	arg1	overproliferation					239:255	mesangial cell overproliferation	224:255	mesangial cell overproliferation	224:255	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	6	42	theme	MPGN	1379:1382	arg1	rats					1384:1387	MPGN rats	1379:1387	MPGN rats	1379:1387	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	7	43	theme	growth	1578:1583	arg1	agonist.Conclusions					1610:1628	the PI3K agonist.Conclusions	1601:1628	the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling	1601:1754	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	7	43	theme	growth	1578:1583	arg1	IGF-1					1593:1597	IGF-1	1593:1597	IGF-1	1593:1597	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	7	43	theme	growth	1578:1583	arg1	factor					1585:1590	insulin-like growth factor	1565:1590	insulin-like growth factor (IGF-1)	1565:1598	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	6	44	theme	urinary	1282:1288	arg1	protein					1290:1296	24 h urinary protein	1277:1296	24 h urinary protein	1277:1296	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	3	45	theme	cell	640:643	arg1	overproliferation					645:661	mesangial cell overproliferation	630:661	mesangial cell overproliferation	630:661	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	7	46	theme	cell	1676:1679	arg1	overproliferation					1681:1697	mesangial cell overproliferation	1666:1697	mesangial cell overproliferation	1666:1697	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	7	47	theme	inhibitory	1515:1524	arg1	effects					1526:1532	the inhibitory effects	1511:1532	the inhibitory effects of AF on MPGN	1511:1546	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	3	48	from	mechanism	552:560	arg1	Design					585:590	MPGN.Research Design	571:590	MPGN.Research Design	571:590	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	5	49	theme	HMCs	1198:1201	arg1	fibrosis					1174:1181	The fibrosis	1170:1181	The fibrosis of LPS-treated HMCs and MPGN rats	1170:1215	The fibrosis of LPS-treated HMCs and MPGN rats was also reduced by AF.
36780316	6	50	theme	h	1280:1280	arg1	protein					1290:1296	24 h urinary protein	1277:1296	24 h urinary protein	1277:1296	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	3	51	theme	AF	624:625	arg1	effects					613:619	the effects	609:619	the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis	609:847	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	52	theme	both in	947:953	arg1	vitro and in					955:966	inflammation both in vitro and in	934:966	inflammation both in vitro and in	934:966	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	6	53	theme	24	1277:1278	arg1	h					1280:1280	h	1280:1280	h	1280:1280	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	3	54	theme	MPGN.Research	571:583	arg1	Design					585:590	MPGN.Research Design	571:590	MPGN.Research Design	571:590	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	7	55	dep	agonist.Conclusions	1610:1628	arg1	alleviates					1634:1643	alleviates	1634:1643	alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling	1634:1754	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	1	56	theme	glomerulonephritis	129:146	arg1	type					121:124	 The most common type	104:124	 The most common type of glomerulonephritis in China	104:155	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	0	57	theme	PI3K/AKT/NF-κB	69:82	arg1	pathway					84:90	PI3K/AKT/NF-κB pathway	69:90	PI3K/AKT/NF-κB pathway	69:90	Albiflorin ameliorates mesangial proliferative glomerulonephritis by PI3K/AKT/NF-κB pathway.
36780316	3	58	theme	mesangial	778:786	arg1	cells					788:792	human mesangial cells	772:792	human mesangial cells (HMCs)	772:799	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	58	theme	mesangial	778:786	arg1	HMCs					795:798	HMCs	795:798	HMCs	795:798	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	7	59	theme	insulin-like	1565:1576	arg1	agonist.Conclusions					1610:1628	the PI3K agonist.Conclusions	1601:1628	the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling	1601:1754	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	7	59	theme	insulin-like	1565:1576	arg1	IGF-1					1593:1597	IGF-1	1593:1597	IGF-1	1593:1597	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	7	59	theme	insulin-like	1565:1576	arg1	factor					1585:1590	insulin-like growth factor	1565:1590	insulin-like growth factor (IGF-1)	1565:1598	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	3	60	theme	work	506:509	arg1	aims					511:514	 Our work aims	501:514	 Our work aims	501:514	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	1	61	from	type	121:124	arg1	China					151:155	China	151:155	China	151:155	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	7	62	from	effects	1526:1532	arg1	MPGN					1543:1546	MPGN	1543:1546	MPGN	1543:1546	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	3	63	theme	inflammation	934:945	arg1	vitro and in					955:966	inflammation both in vitro and in	934:966	inflammation both in vitro and in	934:966	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	64	theme	male	805:808	arg1	Analysis					840:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	7	65	theme	PI3K	1605:1608	arg1	agonist.Conclusions					1610:1628	the PI3K agonist.Conclusions	1601:1628	the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling	1601:1754	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	7	65	theme	PI3K	1605:1608	arg1	factor					1585:1590	insulin-like growth factor	1565:1590	insulin-like growth factor (IGF-1)	1565:1598	Additionally, we found that the protective effects of AF were accompanied by the blocking of PI3K/AKT/NF-κB pathway, and the inhibitory effects of AF on MPGN were reversed by insulin-like growth factor (IGF-1), the PI3K agonist.Conclusions: AF alleviates MPGN via restraining mesangial cell overproliferation, inflammation, and fibrosis via PI3K/AKT/NF-κB signaling.
36780316	3	66	from	effect	472:477	arg1	MPGN.Purpose					488:499	MPGN.Purpose	488:499	MPGN.Purpose	488:499	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	4	67	theme	cytokines	1127:1135	arg1	contents					1102:1109	contents	1102:1109	contents	1102:1109	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	4	67	theme	cytokines	1127:1135	arg1	expression					1088:1097	mRNA expression	1083:1097	mRNA expression	1083:1097	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	3	68	theme	rats.Data	830:838	arg1	Analysis					840:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	69	theme	Sprague	810:816	arg1	Analysis					840:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	male Sprague Dawley (SD) rats.Data Analysis	805:847	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	3	70	theme	AF	482:483	arg1	effect					472:477	the effect	468:477	the effect of AF on MPGN.Purpose	468:499	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	1	71	theme	mesangial	160:168	arg1	MPGN					204:207	MPGN	204:207	MPGN	204:207	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	1	71	theme	mesangial	160:168	arg1	glomerulonephritis					184:201	mesangial proliferative glomerulonephritis	160:201	mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis	160:293	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	1	71	theme	mesangial	160:168	arg1	Background					93:102	Background	93:102	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.	93:294	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	4	72	theme	MPGN	1064:1067	arg1	rats					1069:1072	MPGN rats	1064:1072	MPGN rats	1064:1072	In detail, AF decreased the ki67 expression in lipopolysaccharides (LPS)-treated HMCs and MPGN rats, and the mRNA expression or contents of inflammatory cytokines were reduced after AF treatment.
36780316	5	73	theme	MPGN	1207:1210	arg1	fibrosis					1174:1181	The fibrosis	1170:1181	The fibrosis of LPS-treated HMCs and MPGN rats	1170:1215	The fibrosis of LPS-treated HMCs and MPGN rats was also reduced by AF.
36780316	3	74	theme	vitro and in	694:705	arg1	vivo and					707:714	fibrosis in vitro and in vivo and	682:714	fibrosis in vitro and in vivo and	682:714	Nevertheless, there is no research reporting the effect of AF on MPGN.Purpose: Our work aims to probe into the role and possible mechanism of AF on MPGN.Research Design: We investigated the effects of AF on mesangial cell overproliferation, inflammation, and fibrosis in vitro and in vivo and identified the related signaling pathways.Study Sample: human mesangial cells (HMCs) and male Sprague Dawley (SD) rats.Data Analysis: SPSS 18.0 was used to analyze the data.Results: AF attenuated the proliferation and inflammation both in vitro and in vivo.
36780316	6	75	theme	rats	1384:1387	arg1	dyslipidemia					1339:1350	dyslipidemia	1339:1350	dyslipidemia	1339:1350	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	6	75	theme	rats	1384:1387	arg1	injury					1369:1374	pathological injury	1356:1374	pathological injury	1356:1374	Moreover, AF effectively restrained 24 h urinary protein, improved kidney function, and mitigated dyslipidemia and pathological injury of MPGN rats.
36780316	1	76	theme	proliferative	170:182	arg1	MPGN					204:207	MPGN	204:207	MPGN	204:207	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	1	76	theme	proliferative	170:182	arg1	glomerulonephritis					184:201	mesangial proliferative glomerulonephritis	160:201	mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis	160:293	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36780316	1	76	theme	proliferative	170:182	arg1	Background					93:102	Background	93:102	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.	93:294	Background: The most common type of glomerulonephritis in China is mesangial proliferative glomerulonephritis (MPGN) featured with mesangial cell overproliferation and inflammation, as well as fibrosis.
36031040	8	0	theme	C3	1474:1475	arg1	contents					1477:1484	C3 contents	1474:1484	C3 contents (in the plasma and head kidney)	1474:1516	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	1	1	with	addition	280:287	arg1	sorokiniana					304:314	Chlorella sorokiniana	294:314	Chlorella sorokiniana	294:314	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	8	2	theme	activity	1438:1445	arg1	levels					1447:1452	LZM activity levels	1434:1452	LZM activity levels (in the plasma)	1434:1468	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	3	3	theme	%	626:626	arg1	meal					638:641	10% Chlorella meal	624:641	10% Chlorella meal	624:641	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	9	4	theme	Aeromonas	1672:1680	arg1	salmonicida					1682:1692	Aeromonas salmonicida	1672:1692	Aeromonas salmonicida (P < 0.05)	1672:1703	Furthermore, dietary administration of Chlorella meal significantly increased the survival rate of fish infected with Aeromonas salmonicida (P < 0.05).
36031040	9	4	theme	Aeromonas	1672:1680	arg1	P < 0.05					1695:1702	P < 0.05	1695:1702	P < 0.05	1695:1702	Furthermore, dietary administration of Chlorella meal significantly increased the survival rate of fish infected with Aeromonas salmonicida (P < 0.05).
36031040	0	5	theme	immune	159:164	arg1	response					166:173	immune response	159:173	immune response	159:173	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	1	6	theme	rainbow	412:418	arg1	trout					420:424	rainbow trout	412:424	rainbow trout	412:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	8	7	from	M	1293:1293	arg1	spleen					1362:1367	the spleen	1358:1367	the spleen	1358:1367	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	7	from	M	1293:1293	arg1	kidney					1314:1319	the head kidney	1305:1319	the head kidney	1305:1319	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	6	8	theme	%	841:841	arg1	Chlorella					843:851	5% Chlorella	840:851	5% Chlorella	840:851	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	6	9	theme	gut	989:991	arg1	histology					993:1001	gut histology	989:1001	gut histology	989:1001	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	3	10	contain	contained	587:595	arg2	%					602:602	5%	601:602	5% Chlorella meal	601:617	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	3	10	contain	contained	587:595	arg2	%					598:598	0%	597:598	0%	597:598	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	3	10	contain	contained	587:595	arg1	diet					582:585	10% Chlorella diet	568:585	10% Chlorella diet	568:585	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	3	10	contain	contained	587:595	arg2	meal					638:641	10% Chlorella meal	624:641	10% Chlorella meal	624:641	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	3	10	contain	contained	587:595	arg1	diet					558:561	5% Chlorella diet	545:561	5% Chlorella diet	545:561	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	3	10	contain	contained	587:595	arg1	diet					539:542	The control diet	527:542	The control diet	527:542	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	1	11	theme	antioxidant	347:357	arg1	capacity					359:366	antioxidant capacity	347:366	antioxidant capacity	347:366	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	3	12	theme	Chlorella	548:556	arg1	diet					558:561	5% Chlorella diet	545:561	5% Chlorella diet	545:561	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	3	13	theme	5	545:545	arg1	%					546:546	%	546:546	%	546:546	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	8	14	theme	LPS	1528:1530	arg1	P < 0.05					1543:1550	P < 0.05	1543:1550	P < 0.05	1543:1550	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	14	theme	LPS	1528:1530	arg1	challenge					1532:1540	the LPS challenge	1524:1540	the LPS challenge (P < 0.05)	1524:1551	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	0	15	theme	gut	126:128	arg1	histology					130:138	gut histology	126:138	gut histology	126:138	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	3	16	theme	10	568:569	arg1	%					570:570	%	570:570	%	570:570	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	2	17	theme	proximate	452:460	arg1	composition					462:472	similar proximate composition	444:472	similar proximate composition	444:472	Three diets with similar proximate composition and different Chlorella meal levels were formulated.
36031040	8	18	theme	head	1309:1312	arg1	kidney					1314:1319	the head kidney	1305:1319	the head kidney	1305:1319	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	19	theme	Chlorella	1183:1191	arg1	meal					1193:1196	Chlorella meal	1183:1196	Chlorella meal	1183:1196	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	10	20	theme	fish	1759:1762	arg1	growth					1764:1769	fish growth	1759:1769	fish growth	1759:1769	In conclusion, C. sorokiniana can be used to improve fish growth, antioxidant capacity, and immunity.
36031040	1	21	from	growth	324:329	arg1	trout					420:424	rainbow trout	412:424	rainbow trout	412:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	0	22	theme	rainbow	63:69	arg1	trout					71:75	juvenile rainbow trout	54:75	juvenile rainbow trout (Oncorhynchus mykiss)	54:97	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	22	theme	rainbow	63:69	arg1	mykiss					91:96	Oncorhynchus mykiss	78:96	Oncorhynchus mykiss	78:96	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	2	23	theme	Chlorella	488:496	arg1	meal					498:501	different Chlorella meal	478:501	different Chlorella meal	478:501	Three diets with similar proximate composition and different Chlorella meal levels were formulated.
36031040	8	24	theme	complement	1326:1335	arg1	component					1337:1345	complement component 3	1326:1347	complement component 3 (C3)	1326:1352	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	24	theme	complement	1326:1335	arg1	C3					1350:1351	C3	1350:1351	C3	1350:1351	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	25	theme	lysozyme	1222:1229	arg1	levels					1246:1251	lysozyme (LZM) activity levels	1222:1251	lysozyme (LZM) activity levels (in the head kidney)	1222:1272	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	1	26	from	capacity	359:366	arg1	trout					420:424	rainbow trout	412:424	rainbow trout	412:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	1	27	theme	fish	319:322	arg1	growth					324:329	fish growth	319:329	fish growth	319:329	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	0	28	theme	Aeromonas	207:215	arg1	salmonicida					217:227	Aeromonas salmonicida	207:227	Aeromonas salmonicida	207:227	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	8	29	theme	LZM	1232:1234	arg1	levels					1246:1251	lysozyme (LZM) activity levels	1222:1251	lysozyme (LZM) activity levels (in the head kidney)	1222:1272	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	6	30	dep	intake	894:899	arg1	rate					926:929	rate	926:929	rate	926:929	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	0	31	theme	juvenile	54:61	arg1	trout					71:75	juvenile rainbow trout	54:75	juvenile rainbow trout (Oncorhynchus mykiss)	54:97	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	31	theme	juvenile	54:61	arg1	mykiss					91:96	Oncorhynchus mykiss	78:96	Oncorhynchus mykiss	78:96	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	8	32	theme	activity	1237:1244	arg1	levels					1246:1251	lysozyme (LZM) activity levels	1222:1251	lysozyme (LZM) activity levels (in the head kidney)	1222:1272	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	3	33	dep	%	602:602	arg1	meal					614:617	Chlorella meal	604:617	5% Chlorella meal	601:617	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	0	34	theme	Chlorella	12:20	arg1	meal					34:37	Chlorella sorokiniana meal	12:37	Chlorella sorokiniana meal	12:37	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	2	35	dep	composition	462:472	arg1	levels					503:508	levels	503:508	levels	503:508	Three diets with similar proximate composition and different Chlorella meal levels were formulated.
36031040	0	36	theme	meal	34:37	arg1	Addition					0:7	Addition	0:7	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.	0:228	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	9	37	theme	Chlorella	1593:1601	arg1	meal					1603:1606	Chlorella meal	1593:1606	Chlorella meal	1593:1606	Furthermore, dietary administration of Chlorella meal significantly increased the survival rate of fish infected with Aeromonas salmonicida (P < 0.05).
36031040	3	38	theme	Chlorella	572:580	arg1	diet					582:585	10% Chlorella diet	568:585	10% Chlorella diet	568:585	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	1	39	theme	dietary	272:278	arg1	addition					280:287	dietary addition	272:287	dietary addition with Chlorella sorokiniana	272:314	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	6	40	theme	feed	969:972	arg1	efficiency					974:983	feed efficiency	969:983	feed efficiency	969:983	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	6	41	from	addition	828:835	arg1	diet					860:863	the diet	856:863	the diet	856:863	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	7	42	contain	containing	1010:1019	arg2	meal					1031:1034	Chlorella meal	1021:1034	Chlorella meal	1021:1034	Diets containing Chlorella meal significantly decreased malonaldehyde contents in the plasma after the lipopolysaccharide (LPS) challenge (P < 0.05).
36031040	7	42	contain	containing	1010:1019	arg1	Diets					1004:1008	Diets	1004:1008	Diets containing Chlorella meal	1004:1034	Diets containing Chlorella meal significantly decreased malonaldehyde contents in the plasma after the lipopolysaccharide (LPS) challenge (P < 0.05).
36031040	4	43	theme	triplicate	684:693	arg1	tanks					695:699	triplicate tanks	684:699	triplicate tanks containing 30 fish (165.3 ± 0.6 g) in each tank	684:747	Each diet was assigned to triplicate tanks containing 30 fish (165.3 ± 0.6 g) in each tank.
36031040	8	44	theme	LPS	1390:1392	arg1	challenge					1394:1402	the LPS challenge	1386:1402	the LPS challenge	1386:1402	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	45	theme	M	1293:1293	arg1	contents					1370:1377	immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents	1278:1377	immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents	1278:1377	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	0	46	dep	Addition	0:7	arg1	Influence					100:108	Influence	100:108	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.	0:228	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	47	theme	oxidative	141:149	arg1	stress					151:156	oxidative stress	141:156	oxidative stress	141:156	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	8	48	from	levels	1246:1251	arg1	kidney					1266:1271	the head kidney	1257:1271	the head kidney	1257:1271	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	7	49	theme	lipopolysaccharide	1107:1124	arg1	challenge					1132:1140	the lipopolysaccharide (LPS) challenge	1103:1140	the lipopolysaccharide (LPS) challenge (P < 0.05)	1103:1151	Diets containing Chlorella meal significantly decreased malonaldehyde contents in the plasma after the lipopolysaccharide (LPS) challenge (P < 0.05).
36031040	7	49	theme	lipopolysaccharide	1107:1124	arg1	P < 0.05					1143:1150	P < 0.05	1143:1150	P < 0.05	1143:1150	Diets containing Chlorella meal significantly decreased malonaldehyde contents in the plasma after the lipopolysaccharide (LPS) challenge (P < 0.05).
36031040	9	50	theme	fish	1653:1656	arg1	rate					1645:1648	the survival rate	1632:1648	the survival rate of fish infected with Aeromonas salmonicida (P < 0.05)	1632:1703	Furthermore, dietary administration of Chlorella meal significantly increased the survival rate of fish infected with Aeromonas salmonicida (P < 0.05).
36031040	3	51	theme	Chlorella	604:612	arg1	meal					614:617	Chlorella meal	604:617	5% Chlorella meal	601:617	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	1	52	from	effects	261:267	arg1	resistance					398:407	disease resistance	390:407	disease resistance in rainbow trout	390:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	1	52	from	effects	261:267	arg1	growth					324:329	fish growth	319:329	fish growth	319:329	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	1	52	from	effects	261:267	arg1	histology					336:344	gut histology	332:344	gut histology	332:344	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	1	52	from	effects	261:267	arg1	capacity					359:366	antioxidant capacity	347:366	antioxidant capacity	347:366	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	1	52	from	effects	261:267	arg1	response					376:383	immune response	369:383	immune response	369:383	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	1	53	theme	disease	390:396	arg1	resistance					398:407	disease resistance	390:407	disease resistance in rainbow trout	390:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	8	54	theme	LZM	1434:1436	arg1	levels					1447:1452	LZM activity levels	1434:1452	LZM activity levels (in the plasma)	1434:1468	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	3	55	theme	Chlorella	628:636	arg1	meal					638:641	10% Chlorella meal	624:641	10% Chlorella meal	624:641	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	3	56	theme	10	624:625	arg1	%					626:626	%	626:626	%	626:626	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	8	57	from	contents	1370:1377	arg1	kidney					1266:1271	the head kidney	1257:1271	the head kidney	1257:1271	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	3	58	theme	control	531:537	arg1	diet					539:542	The control diet	527:542	The control diet	527:542	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	6	59	theme	Chlorella	843:851	arg1	addition					828:835	the addition	824:835	the addition of 5% Chlorella in the diet	824:863	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	8	60	from	contents	1477:1484	arg1	plasma					1462:1467	the plasma	1458:1467	the plasma	1458:1467	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	60	from	contents	1477:1484	arg1	kidney					1510:1515	the plasma and head kidney	1490:1515	kidney	1510:1515	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	61	theme	plasma	1494:1499	arg1	kidney					1510:1515	the plasma and head kidney	1490:1515	kidney	1510:1515	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	6	62	theme	5	840:840	arg1	%					841:841	%	841:841	%	841:841	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	0	63	theme	fish	113:116	arg1	growth					118:123	fish growth	113:123	fish growth	113:123	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	3	64	theme	%	546:546	arg1	diet					558:561	5% Chlorella diet	545:561	5% Chlorella diet	545:561	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	8	65	theme	head	1505:1508	arg1	kidney					1510:1515	the plasma and head kidney	1490:1515	kidney	1510:1515	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	7	66	theme	malonaldehyde	1060:1072	arg1	contents					1074:1081	malonaldehyde contents	1060:1081	malonaldehyde contents	1060:1081	Diets containing Chlorella meal significantly decreased malonaldehyde contents in the plasma after the lipopolysaccharide (LPS) challenge (P < 0.05).
36031040	0	67	from	Addition	0:7	arg1	diet					46:49	the diet	42:49	the diet of juvenile rainbow trout (Oncorhynchus mykiss)	42:97	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	1	68	theme	immune	369:374	arg1	response					376:383	immune response	369:383	immune response	369:383	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	2	69	with	diets	433:437	arg1	meal					498:501	different Chlorella meal	478:501	different Chlorella meal	478:501	Three diets with similar proximate composition and different Chlorella meal levels were formulated.
36031040	2	69	with	diets	433:437	arg1	composition					462:472	similar proximate composition	444:472	similar proximate composition	444:472	Three diets with similar proximate composition and different Chlorella meal levels were formulated.
36031040	8	70	theme	Dietary	1154:1160	arg1	supplementation					1162:1176	Dietary supplementation	1154:1176	Dietary supplementation with Chlorella meal	1154:1196	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	4	71	contain	containing	701:710	arg1	tanks					695:699	triplicate tanks	684:699	triplicate tanks containing 30 fish (165.3 ± 0.6 g) in each tank	684:747	Each diet was assigned to triplicate tanks containing 30 fish (165.3 ± 0.6 g) in each tank.
36031040	4	71	contain	containing	701:710	arg2	165.3 ± 0.6 g					721:733	165.3 ± 0.6 g	721:733	165.3 ± 0.6 g	721:733	Each diet was assigned to triplicate tanks containing 30 fish (165.3 ± 0.6 g) in each tank.
36031040	4	71	contain	containing	701:710	arg2	fish					715:718	30 fish	712:718	30 fish (165.3 ± 0.6 g)	712:734	Each diet was assigned to triplicate tanks containing 30 fish (165.3 ± 0.6 g) in each tank.
36031040	1	72	from	response	376:383	arg1	trout					420:424	rainbow trout	412:424	rainbow trout	412:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	5	73	theme	experimental	764:775	arg1	diets					777:781	experimental diets	764:781	experimental diets	764:781	Fish were fed experimental diets for ninety days.
36031040	6	74	theme	feed	889:892	arg1	intake					894:899	feed intake	889:899	feed intake by 19.3%	889:908	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	2	75	theme	similar	444:450	arg1	composition					462:472	similar proximate composition	444:472	similar proximate composition	444:472	Three diets with similar proximate composition and different Chlorella meal levels were formulated.
36031040	0	76	theme	trout	71:75	arg1	diet					46:49	the diet	42:49	the diet of juvenile rainbow trout (Oncorhynchus mykiss)	42:97	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	1	77	theme	Chlorella	294:302	arg1	sorokiniana					304:314	Chlorella sorokiniana	294:314	Chlorella sorokiniana	294:314	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	8	78	theme	component	1337:1345	arg1	contents					1370:1377	immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents	1278:1377	immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents	1278:1377	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	0	79	theme	Oncorhynchus	78:89	arg1	trout					71:75	juvenile rainbow trout	54:75	juvenile rainbow trout (Oncorhynchus mykiss)	54:97	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	79	theme	Oncorhynchus	78:89	arg1	mykiss					91:96	Oncorhynchus mykiss	78:96	Oncorhynchus mykiss	78:96	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	80	theme	disease	180:186	arg1	resistance					188:197	disease resistance	180:197	disease resistance against Aeromonas salmonicida	180:227	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	2	81	theme	different	478:486	arg1	meal					498:501	different Chlorella meal	478:501	different Chlorella meal	478:501	Three diets with similar proximate composition and different Chlorella meal levels were formulated.
36031040	4	82	theme	Each	658:661	arg1	diet					663:666	Each diet	658:666	Each diet	658:666	Each diet was assigned to triplicate tanks containing 30 fish (165.3 ± 0.6 g) in each tank.
36031040	10	83	theme	antioxidant	1772:1782	arg1	capacity					1784:1791	antioxidant capacity	1772:1791	antioxidant capacity	1772:1791	In conclusion, C. sorokiniana can be used to improve fish growth, antioxidant capacity, and immunity.
36031040	1	84	from	histology	336:344	arg1	trout					420:424	rainbow trout	412:424	rainbow trout	412:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	8	85	from	levels	1447:1452	arg1	plasma					1462:1467	the plasma	1458:1467	the plasma	1458:1467	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	85	from	levels	1447:1452	arg1	kidney					1510:1515	the plasma and head kidney	1490:1515	kidney	1510:1515	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	7	86	theme	Chlorella	1021:1029	arg1	meal					1031:1034	Chlorella meal	1021:1034	Chlorella meal	1021:1034	Diets containing Chlorella meal significantly decreased malonaldehyde contents in the plasma after the lipopolysaccharide (LPS) challenge (P < 0.05).
36031040	1	87	theme	gut	332:334	arg1	histology					336:344	gut histology	332:344	gut histology	332:344	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	0	88	theme	sorokiniana	22:32	arg1	meal					34:37	Chlorella sorokiniana meal	12:37	Chlorella sorokiniana meal	12:37	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	6	89	theme	weight	914:919	arg1	gain					921:924	weight gain	914:924	weight gain	914:924	The results showed that the addition of 5% Chlorella in the diet significantly increased feed intake by 19.3% and weight gain rate by 17.3% (P < 0.05) without affecting feed efficiency and gut histology.
36031040	9	90	theme	dietary	1567:1573	arg1	administration					1575:1588	dietary administration	1567:1588	dietary administration of Chlorella meal	1567:1606	Furthermore, dietary administration of Chlorella meal significantly increased the survival rate of fish infected with Aeromonas salmonicida (P < 0.05).
36031040	8	91	with	supplementation	1162:1176	arg1	meal					1193:1196	Chlorella meal	1183:1196	Chlorella meal	1183:1196	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	3	92	theme	%	570:570	arg1	diet					582:585	10% Chlorella diet	568:585	10% Chlorella diet	568:585	The control diet, 5% Chlorella diet, and 10% Chlorella diet contained 0%, 5% Chlorella meal, and 10% Chlorella meal, respectively.
36031040	9	93	theme	meal	1603:1606	arg1	administration					1575:1588	dietary administration	1567:1588	dietary administration of Chlorella meal	1567:1606	Furthermore, dietary administration of Chlorella meal significantly increased the survival rate of fish infected with Aeromonas salmonicida (P < 0.05).
36031040	8	94	theme	head	1261:1264	arg1	kidney					1266:1271	the head kidney	1257:1271	the head kidney	1257:1271	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	10	95	used	used	1743:1746	arg2	sorokiniana					1724:1734	C. sorokiniana	1721:1734	C. sorokiniana	1721:1734	In conclusion, C. sorokiniana can be used to improve fish growth, antioxidant capacity, and immunity.
36031040	1	96	theme	addition	280:287	arg1	effects					261:267	the effects	257:267	the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout	257:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	1	97	from	resistance	398:407	arg1	trout					420:424	rainbow trout	412:424	rainbow trout	412:424	This study aimed to assess the effects of dietary addition with Chlorella sorokiniana on fish growth, gut histology, antioxidant capacity, immune response, and disease resistance in rainbow trout.
36031040	9	98	theme	survival	1636:1643	arg1	rate					1645:1648	the survival rate	1632:1648	the survival rate of fish infected with Aeromonas salmonicida (P < 0.05)	1632:1703	Furthermore, dietary administration of Chlorella meal significantly increased the survival rate of fish infected with Aeromonas salmonicida (P < 0.05).
36031040	0	99	from	Influence	100:108	arg1	stress					151:156	oxidative stress	141:156	oxidative stress	141:156	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	99	from	Influence	100:108	arg1	response					166:173	immune response	159:173	immune response	159:173	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	99	from	Influence	100:108	arg1	resistance					188:197	disease resistance	180:197	disease resistance against Aeromonas salmonicida	180:227	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	99	from	Influence	100:108	arg1	growth					118:123	fish growth	113:123	fish growth	113:123	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	0	99	from	Influence	100:108	arg1	histology					130:138	gut histology	126:138	gut histology	126:138	Addition of Chlorella sorokiniana meal in the diet of juvenile rainbow trout (Oncorhynchus mykiss): Influence on fish growth, gut histology, oxidative stress, immune response, and disease resistance against Aeromonas salmonicida.
36031040	8	100	theme	immunoglobulin	1278:1291	arg1	IgM					1296:1298	IgM	1296:1298	IgM	1296:1298	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	100	theme	immunoglobulin	1278:1291	arg1	M					1293:1293	immunoglobulin M	1278:1293	immunoglobulin M (IgM) (in the head kidney)	1278:1320	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	101	from	component	1337:1345	arg1	spleen					1362:1367	the spleen	1358:1367	the spleen	1358:1367	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36031040	8	101	from	component	1337:1345	arg1	kidney					1314:1319	the head kidney	1305:1319	the head kidney	1305:1319	Dietary supplementation with Chlorella meal significantly increased lysozyme (LZM) activity levels (in the head kidney) and immunoglobulin M (IgM) (in the head kidney) and complement component 3 (C3) (in the spleen) contents before the LPS challenge, and simultaneously increased LZM activity levels (in the plasma) and C3 contents (in the plasma and head kidney) after the LPS challenge (P < 0.05).
36737950	0	0	theme	Colitis	85:91	arg1	Model					93:97	DSS-induced Mice Colitis Model	68:97	DSS-induced Mice Colitis Model	68:97	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	3	1	theme	R.	264:265	arg1	cv					274:275	blooming R. rugosa cv	255:275	blooming R. rugosa cv	255:275	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	2	theme	rose	188:191	arg1	mixture					219:225	a mixture	217:225	a mixture of compounds extracted from blooming R. rugosa cv	217:275	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	2	theme	rose	188:191	arg1	PREO					208:211	PREO	208:211	PREO	208:211	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	2	theme	rose	188:191	arg1	oil					203:205	Pingyin rose essential oil	180:205	Pingyin rose essential oil (PREO)	180:212	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	8	3	theme	dismutases	847:856	arg1	activities					822:831	the enzyme activities	811:831	the enzyme activities of superoxide dismutases (SOD)	811:862	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	3	theme	dismutases	847:856	arg1	proteins					798:805	tight junction proteins	783:805	tight junction proteins	783:805	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	6	4	theme	-induced	533:540	arg1	integrity					550:558	dextran sodium sulfate (DSS)-induced barrier integrity	505:558	dextran sodium sulfate (DSS)-induced barrier integrity damages	505:566	We aimed to decipher if the PREO could alleviate and restore dextran sodium sulfate (DSS)-induced barrier integrity damages.
36737950	5	5	theme	elegant	294:300	arg1	smell					302:306	its elegant smell	290:306	its elegant smell	290:306	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	0	6	theme	Mice	80:83	arg1	Colitis					85:91	DSS-induced Mice Colitis	68:91	DSS-induced Mice Colitis Model	68:97	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	3	7	theme	essential	193:201	arg1	mixture					219:225	a mixture	217:225	a mixture of compounds extracted from blooming R. rugosa cv	217:275	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	7	theme	essential	193:201	arg1	PREO					208:211	PREO	208:211	PREO	208:211	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	7	theme	essential	193:201	arg1	oil					203:205	Pingyin rose essential oil	180:205	Pingyin rose essential oil (PREO)	180:212	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	10	8	theme	microbiota	1208:1217	arg1	composition					1185:1195	the composition	1181:1195	the composition of the gut microbiota	1181:1217	Further, PREO modulated the composition of the gut microbiota and Spearman's correlation analysis revealed a positive effect.
36737950	8	9	theme	nitric	900:905	arg1	NO					914:915	NO	914:915	NO	914:915	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	9	theme	nitric	900:905	arg1	oxide					907:911	nitric oxide	900:911	nitric oxide (NO)	900:916	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	9	10	theme	pro-inflammatory	1068:1083	arg1	IL-6					1151:1154	IL-6	1151:1154	IL-6	1151:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	10	theme	pro-inflammatory	1068:1083	arg1	IL					1139:1140	interleukin (IL)-1β	1126:1144	interleukin (IL)-1β	1126:1144	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	10	theme	pro-inflammatory	1068:1083	arg1	cytokines					1085:1093	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	10	theme	pro-inflammatory	1068:1083	arg1	factor					1110:1115	tumor necrosis factor	1095:1115	tumor necrosis factor (TNF-α)	1095:1123	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	8	11	theme	PREO-treated	992:1003	arg1	mice					1019:1022	PREO-treated C57BL6 female mice	992:1022	PREO-treated C57BL6 female mice	992:1022	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	12	theme	oxide	907:911	arg1	production					968:977	nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production	900:977	nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production	900:977	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	10	13	theme	gut	1204:1206	arg1	microbiota					1208:1217	the gut microbiota	1200:1217	the gut microbiota	1200:1217	Further, PREO modulated the composition of the gut microbiota and Spearman's correlation analysis revealed a positive effect.
36737950	8	14	theme	proteins	798:805	arg1	expression					769:778	The expression	765:778	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD)	765:862	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	14	theme	proteins	798:805	arg1	CAT					879:881	CAT	879:881	CAT	879:881	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	14	theme	proteins	798:805	arg1	catalase					869:876	catalase	869:876	catalase (CAT)	869:882	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	15	theme	superoxide	836:845	arg1	SOD					859:861	SOD	859:861	SOD	859:861	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	15	theme	superoxide	836:845	arg1	dismutases					847:856	superoxide dismutases	836:856	superoxide dismutases (SOD)	836:862	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	2	16	theme	homologous	133:142	arg1	Plena					116:120	Plena	116:120	Plena	116:120	Plena is a 'drug homologous food' in China with a long history.
36737950	2	16	theme	homologous	133:142	arg1	food					144:147	drug homologous food	128:147	a 'drug homologous food' in China with a long history	125:177	Plena is a 'drug homologous food' in China with a long history.
36737950	3	17	theme	Pingyin	180:186	arg1	mixture					219:225	a mixture	217:225	a mixture of compounds extracted from blooming R. rugosa cv	217:275	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	17	theme	Pingyin	180:186	arg1	PREO					208:211	PREO	208:211	PREO	208:211	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	17	theme	Pingyin	180:186	arg1	oil					203:205	Pingyin rose essential oil	180:205	Pingyin rose essential oil (PREO)	180:212	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	9	18	theme	PREO	1025:1028	arg1	treatment					1030:1038	PREO treatment	1025:1038	PREO treatment	1025:1038	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	7	19	theme	weight	716:721	arg1	loss					723:726	weight loss	716:726	weight loss	716:726	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	5	20	theme	excellent	312:320	arg1	effects					322:328	excellent effects	312:328	excellent effects	312:328	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	8	21	theme	female	1012:1017	arg1	mice					1019:1022	PREO-treated C57BL6 female mice	992:1022	PREO-treated C57BL6 female mice	992:1022	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	2	22	theme	drug	128:131	arg1	Plena					116:120	Plena	116:120	Plena	116:120	Plena is a 'drug homologous food' in China with a long history.
36737950	2	22	theme	drug	128:131	arg1	food					144:147	drug homologous food	128:147	a 'drug homologous food' in China with a long history	125:177	Plena is a 'drug homologous food' in China with a long history.
36737950	7	23	theme	colon	747:751	arg1	shortening					753:762	colon shortening	747:762	colon shortening	747:762	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	8	24	theme	C57BL6	1005:1010	arg1	mice					1019:1022	PREO-treated C57BL6 female mice	992:1022	PREO-treated C57BL6 female mice	992:1022	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	10	25	theme	correlation	1234:1244	arg1	analysis					1246:1253	Spearman's correlation analysis	1223:1253	Spearman's correlation analysis	1223:1253	Further, PREO modulated the composition of the gut microbiota and Spearman's correlation analysis revealed a positive effect.
36737950	9	26	theme	tumor	1095:1099	arg1	TNF-α					1118:1122	TNF-α	1118:1122	TNF-α	1118:1122	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	26	theme	tumor	1095:1099	arg1	cytokines					1085:1093	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	26	theme	tumor	1095:1099	arg1	factor					1110:1115	tumor necrosis factor	1095:1115	tumor necrosis factor (TNF-α)	1095:1123	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	12	27	theme	additive	1565:1572	arg1	food					1560:1563	a functional food	1547:1563	a functional food additive	1547:1572	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	12	27	theme	additive	1565:1572	arg1	PREO					1476:1479	PREO	1476:1479	PREO	1476:1479	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	0	28	theme	Rose	8:11	arg1	Oil					23:25	Pingyin Rose Essential Oil	0:25	Pingyin Rose Essential Oil	0:25	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	3	29	theme	rugosa	267:272	arg1	cv					274:275	blooming R. rugosa cv	255:275	blooming R. rugosa cv	255:275	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	12	30	theme	gut	1514:1516	arg1	disorders					1518:1526	gut disorders	1514:1526	gut disorders	1514:1526	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	0	31	theme	Pingyin	0:6	arg1	Oil					23:25	Pingyin Rose Essential Oil	0:25	Pingyin Rose Essential Oil	0:25	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	11	32	theme	intestinal	1372:1381	arg1	dysfunction					1391:1401	intestinal barrier dysfunction	1372:1401	intestinal barrier dysfunction in this study	1372:1415	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	8	33	theme	tight	783:787	arg1	proteins					798:805	tight junction proteins	783:805	tight junction proteins	783:805	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	7	34	theme	15 μL/kg	607:614	arg1	treatment					617:625	a 7-day PREO (15 μL/kg) treatment	593:625	a 7-day PREO (15 μL/kg) treatment	593:625	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	1	35	theme	Rosa	100:103	arg1	cv					112:113	Rosa rugosa cv	100:113	Rosa rugosa cv.	100:114	Rosa rugosa cv.
36737950	7	36	theme	occult	729:734	arg1	blood					736:740	occult blood	729:740	occult blood	729:740	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	9	37	theme	cytokines	1085:1093	arg1	expression					1054:1063	the expression	1050:1063	the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1050:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	0	38	theme	Essential	13:21	arg1	Oil					23:25	Pingyin Rose Essential Oil	0:25	Pingyin Rose Essential Oil	0:25	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	8	39	theme	malondialdehyde	919:933	arg1	production					968:977	nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production	900:977	nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production	900:977	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	9	40	theme	necrosis	1101:1108	arg1	TNF-α					1118:1122	TNF-α	1118:1122	TNF-α	1118:1122	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	40	theme	necrosis	1101:1108	arg1	cytokines					1085:1093	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	40	theme	necrosis	1101:1108	arg1	factor					1110:1115	tumor necrosis factor	1095:1115	tumor necrosis factor (TNF-α)	1095:1123	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	1	41	theme	rugosa	105:110	arg1	cv					112:113	Rosa rugosa cv	100:113	Rosa rugosa cv.	100:114	Rosa rugosa cv.
36737950	5	42	theme	food	407:410	arg1	industry					412:419	the food industry	403:419	the food industry	403:419	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	0	43	theme	Intestinal	36:45	arg1	Integrity					55:63	Intestinal Barrier Integrity	36:63	Intestinal Barrier Integrity	36:63	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	5	44	dep	stress	343:348	arg1	alleviation					367:377	alleviation	367:377	alleviation	367:377	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	9	45	theme	interleukin	1126:1136	arg1	IL					1139:1140	interleukin (IL)-1β	1126:1144	interleukin (IL)-1β	1126:1144	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	45	theme	interleukin	1126:1136	arg1	cytokines					1085:1093	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	8	46	theme	junction	789:796	arg1	proteins					798:805	tight junction proteins	783:805	tight junction proteins	783:805	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	11	47	theme	barrier	1383:1389	arg1	dysfunction					1391:1401	intestinal barrier dysfunction	1372:1401	intestinal barrier dysfunction in this study	1372:1415	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	12	48	theme	functional	1549:1558	arg1	food					1560:1563	a functional food	1547:1563	a functional food additive	1547:1572	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	12	48	theme	functional	1549:1558	arg1	PREO					1476:1479	PREO	1476:1479	PREO	1476:1479	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	7	49	theme	index	689:693	arg1	scores					701:706	disease activity index (DAI) scores	672:706	disease activity index (DAI) scores	672:706	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	5	50	from	smell	302:306	arg1	inflammation					354:365	inflammation	354:365	inflammation	354:365	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	5	50	from	smell	302:306	arg1	stress					343:348	oxidative stress	333:348	oxidative stress	333:348	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	7	51	theme	7-day	595:599	arg1	treatment					617:625	a 7-day PREO (15 μL/kg) treatment	593:625	a 7-day PREO (15 μL/kg) treatment	593:625	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	7	52	theme	colitis	642:648	arg1	symptoms					650:657	the colitis symptoms	638:657	the colitis symptoms	638:657	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	11	53	theme	TLR4-NF-kB	1425:1434	arg1	pathway					1446:1452	the TLR4-NF-kB signaling pathway	1421:1452	the TLR4-NF-kB signaling pathway	1421:1452	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	12	54	theme	preventive	1485:1494	arg1	potential					1496:1504	preventive potential	1485:1504	preventive potential against gut disorders	1485:1526	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	7	55	theme	PREO	601:604	arg1	treatment					617:625	a 7-day PREO (15 μL/kg) treatment	593:625	a 7-day PREO (15 μL/kg) treatment	593:625	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	5	56	from	effects	322:328	arg1	inflammation					354:365	inflammation	354:365	inflammation	354:365	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	5	56	from	effects	322:328	arg1	stress					343:348	oxidative stress	333:348	oxidative stress	333:348	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	11	57	from	dysfunction	1391:1401	arg1	study					1411:1415	this study	1406:1415	this study	1406:1415	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	0	58	theme	Barrier	47:53	arg1	Integrity					55:63	Intestinal Barrier Integrity	36:63	Intestinal Barrier Integrity	36:63	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	7	59	theme	disease	672:678	arg1	index					689:693	disease activity index	672:693	disease activity index (DAI) scores	672:706	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	7	59	theme	disease	672:678	arg1	DAI					696:698	DAI	696:698	DAI	696:698	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	3	60	theme	blooming	255:262	arg1	cv					274:275	blooming R. rugosa cv	255:275	blooming R. rugosa cv	255:275	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	8	61	theme	activities	822:831	arg1	expression					769:778	The expression	765:778	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD)	765:862	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	61	theme	activities	822:831	arg1	CAT					879:881	CAT	879:881	CAT	879:881	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	8	61	theme	activities	822:831	arg1	catalase					869:876	catalase	869:876	catalase (CAT)	869:882	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	2	62	with	food	144:147	arg1	history					171:177	a long history	164:177	a long history	164:177	Plena is a 'drug homologous food' in China with a long history.
36737950	11	63	theme	western	1314:1320	arg1	blot					1322:1325	western blot	1314:1325	western blot	1314:1325	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	7	64	theme	activity	680:687	arg1	index					689:693	disease activity index	672:693	disease activity index (DAI) scores	672:706	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	7	64	theme	activity	680:687	arg1	DAI					696:698	DAI	696:698	DAI	696:698	The results showed that a 7-day PREO (15 μL/kg) treatment alleviated the colitis symptoms by improving disease activity index (DAI) scores through weight loss, occult blood, and colon shortening.
36737950	3	65	theme	compounds	230:238	arg1	mixture					219:225	a mixture	217:225	a mixture of compounds extracted from blooming R. rugosa cv	217:275	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	3	65	theme	compounds	230:238	arg1	oil					203:205	Pingyin rose essential oil	180:205	Pingyin rose essential oil (PREO)	180:212	Pingyin rose essential oil (PREO) is a mixture of compounds extracted from blooming R. rugosa cv.
36737950	2	66	theme	long	166:169	arg1	history					171:177	a long history	164:177	a long history	164:177	Plena is a 'drug homologous food' in China with a long history.
36737950	8	67	theme	enzyme	815:820	arg1	activities					822:831	the enzyme activities	811:831	the enzyme activities of superoxide dismutases (SOD)	811:862	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	11	68	theme	blot	1322:1325	arg1	results					1327:1333	The transcriptome analysis and western blot results	1283:1333	The transcriptome analysis and western blot results	1283:1333	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	8	69	theme	myeloperoxidase	946:960	arg1	production					968:977	nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production	900:977	nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production	900:977	The expression of tight junction proteins and the enzyme activities of superoxide dismutases (SOD), and catalase (CAT) increased while nitric oxide (NO), malondialdehyde (MDA), and myeloperoxidase (MPO) production decreased in PREO-treated C57BL6 female mice.
36737950	5	70	used	used	395:398	arg2	additive					434:441	additive	434:441	additive	434:441	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	5	70	used	used	395:398	arg2	PREO					380:383	PREO	380:383	PREO	380:383	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	11	71	theme	transcriptome	1287:1299	arg1	analysis					1301:1308	transcriptome analysis	1287:1308	transcriptome analysis	1287:1308	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	11	72	theme	analysis	1301:1308	arg1	results					1327:1333	The transcriptome analysis and western blot results	1283:1333	The transcriptome analysis and western blot results	1283:1333	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	12	73	contain	has	1481:1483	arg1	food					1560:1563	a functional food	1547:1563	a functional food additive	1547:1572	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	12	73	contain	has	1481:1483	arg1	PREO					1476:1479	PREO	1476:1479	PREO	1476:1479	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	12	73	contain	has	1481:1483	arg2	potential					1496:1504	preventive potential	1485:1504	preventive potential against gut disorders	1485:1526	We hypothesized that PREO has preventive potential against gut disorders and could serve as a functional food additive.
36737950	0	74	theme	DSS-induced	68:78	arg1	Colitis					85:91	DSS-induced Mice Colitis	68:91	DSS-induced Mice Colitis Model	68:97	Pingyin Rose Essential Oil Restores Intestinal Barrier Integrity in DSS-induced Mice Colitis Model.
36737950	6	75	dep	integrity	550:558	arg1	damages					560:566	damages	560:566	damages	560:566	We aimed to decipher if the PREO could alleviate and restore dextran sodium sulfate (DSS)-induced barrier integrity damages.
36737950	5	76	theme	oxidative	333:341	arg1	stress					343:348	oxidative stress	333:348	oxidative stress	333:348	With its elegant smell and excellent effects on oxidative stress and inflammation alleviation, PREO is wildly used in the food industry as a popular additive.
36737950	2	77	from	food	144:147	arg1	China					153:157	China	153:157	China	153:157	Plena is a 'drug homologous food' in China with a long history.
36737950	11	78	theme	signaling	1436:1444	arg1	pathway					1446:1452	the TLR4-NF-kB signaling pathway	1421:1452	the TLR4-NF-kB signaling pathway	1421:1452	The transcriptome analysis and western blot results indicated that PREO might ameliorate intestinal barrier dysfunction in this study via the TLR4-NF-kB signaling pathway.
36737950	10	79	theme	positive	1266:1273	arg1	effect					1275:1280	a positive effect	1264:1280	a positive effect	1264:1280	Further, PREO modulated the composition of the gut microbiota and Spearman's correlation analysis revealed a positive effect.
36737950	6	80	theme	barrier	542:548	arg1	integrity					550:558	dextran sodium sulfate (DSS)-induced barrier integrity	505:558	dextran sodium sulfate (DSS)-induced barrier integrity damages	505:566	We aimed to decipher if the PREO could alleviate and restore dextran sodium sulfate (DSS)-induced barrier integrity damages.
36737950	9	81	dep	cytokines	1085:1093	arg1	IL-6					1151:1154	IL-6	1151:1154	IL-6	1151:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	81	dep	cytokines	1085:1093	arg1	IL					1139:1140	interleukin (IL)-1β	1126:1144	interleukin (IL)-1β	1126:1144	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	81	dep	cytokines	1085:1093	arg1	TNF-α					1118:1122	TNF-α	1118:1122	TNF-α	1118:1122	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	81	dep	cytokines	1085:1093	arg1	cytokines					1085:1093	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6	1068:1154	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36737950	9	81	dep	cytokines	1085:1093	arg1	factor					1110:1115	tumor necrosis factor	1095:1115	tumor necrosis factor (TNF-α)	1095:1123	PREO treatment inhibited the expression of pro-inflammatory cytokines tumor necrosis factor (TNF-α), interleukin (IL)-1β, and IL-6.
36587451	2	0	theme	inflammatory	365:376	arg1	responses					378:386	inflammatory responses	365:386	inflammatory responses	365:386	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	12	1	theme	challenge-induced	2105:2121	arg1	inflammation					2134:2145	the LPS challenge-induced intestinal inflammation	2097:2145	the LPS challenge-induced intestinal inflammation	2097:2145	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	9	2	theme	P	1368:1368	arg1	decline					1359:1365	a significant decline	1345:1365	a significant decline (P < 0.05)	1345:1376	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	9	2	theme	P	1368:1368	arg1	<					1370:1370	P < 0.05	1368:1375	P < 0.05	1368:1375	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	10	3	theme	intestinal	1619:1628	arg1	mucosa					1630:1635	intestinal mucosa	1619:1635	intestinal mucosa	1619:1635	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	4	4	theme	mg/kg	656:660	arg1	quercetin					662:670	0.4 mg/kg quercetin	652:670	0.4 mg/kg quercetin	652:670	LQ group was fed with 0.4 mg/kg quercetin and at the end of 12 wk, LC and LQ groups were challenged intraperitoneally with lipopolysaccharide (LPS).
36587451	7	5	theme	P	1069:1069	arg1	<					1071:1071	P < 0.05	1069:1076	P < 0.05	1069:1076	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	7	5	theme	P	1069:1069	arg1	contents					1059:1066	the elevated malondialdehyde contents	1030:1066	the elevated malondialdehyde contents (P < 0.05)	1030:1077	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	11	6	theme	health-promoting	1897:1912	arg1	Negativicutes					1954:1966	Negativicutes	1954:1966	Negativicutes	1954:1966	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	6	theme	health-promoting	1897:1912	arg1	bacteria					1914:1921	some short chain fatty acids (SCFA)-producing or health-promoting bacteria	1848:1921	some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis	1848:2040	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	6	theme	health-promoting	1897:1912	arg1	Prevotellaceae					1997:2010	Prevotellaceae	1997:2010	Prevotellaceae	1997:2010	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	6	theme	health-promoting	1897:1912	arg1	Bacteroides_salanitronis					2017:2040	Bacteroides_salanitronis	2017:2040	Bacteroides_salanitronis	2017:2040	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	6	theme	health-promoting	1897:1912	arg1	Megamonas					1986:1994	Megamonas	1986:1994	Megamonas	1986:1994	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	4	7	theme	LQ	630:631	arg1	group					633:637	LQ group	630:637	LQ group	630:637	LQ group was fed with 0.4 mg/kg quercetin and at the end of 12 wk, LC and LQ groups were challenged intraperitoneally with lipopolysaccharide (LPS).
36587451	12	8	theme	LPS	2101:2103	arg1	inflammation					2134:2145	the LPS challenge-induced intestinal inflammation	2097:2145	the LPS challenge-induced intestinal inflammation	2097:2145	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	3	9	theme	hens	543:546	arg1	total					479:483	A total	477:483	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens	477:546	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	9	10	theme	mRNA	1385:1388	arg1	expression					1390:1399	the mRNA expression	1381:1399	the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers	1381:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	11	11	theme	bacteria	1914:1921	arg1	abundance					1835:1843	the relative abundance	1822:1843	the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis	1822:2040	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	12	12	theme	increased	2258:2266	arg1	population					2268:2277	the increased population	2254:2277	the increased population of SCFA-producing bacteria	2254:2304	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	11	13	theme	gut	1763:1765	arg1	microenvironment					1767:1782	gut microenvironment	1763:1782	gut microenvironment	1763:1782	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	7	14	theme	elevated	1034:1041	arg1	<					1071:1071	P < 0.05	1069:1076	P < 0.05	1069:1076	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	7	14	theme	elevated	1034:1041	arg1	contents					1059:1066	the elevated malondialdehyde contents	1030:1066	the elevated malondialdehyde contents (P < 0.05)	1030:1077	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	10	15	theme	P	1675:1675	arg1	<					1677:1677	P < 0.05	1675:1682	P < 0.05	1675:1682	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	6	16	theme	intestinal	891:900	arg1	injury					910:915	an obvious intestinal mucosal injury	880:915	an obvious intestinal mucosal injury	880:915	LPS challenge induced an obvious intestinal mucosal injury, necrosis and shedding, while quercetin intervention maintained its structure.
36587451	8	17	theme	LPS-induced	1249:1259	arg1	decreases					1261:1269	the LPS-induced decreases	1245:1269	the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05)	1245:1332	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	11	18	theme	quercetin	1789:1797	arg1	addition					1799:1806	quercetin addition	1789:1806	quercetin addition	1789:1806	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	10	19	theme	dietary	1695:1701	arg1	supplementation					1713:1727	dietary quercetin supplementation	1695:1727	dietary quercetin supplementation	1695:1727	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	4	20	theme	wk	693:694	arg1	end					683:685	the end	679:685	the end of 12 wk	679:694	LQ group was fed with 0.4 mg/kg quercetin and at the end of 12 wk, LC and LQ groups were challenged intraperitoneally with lipopolysaccharide (LPS).
36587451	1	21	from	functions	251:259	arg1	birds					283:287	birds	283:287	birds	283:287	Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds.
36587451	11	22	theme	chain	1859:1863	arg1	SCFA					1878:1881	SCFA	1878:1881	SCFA	1878:1881	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	22	theme	chain	1859:1863	arg1	acids					1871:1875	some short chain fatty acids	1848:1875	some short chain fatty acids (SCFA)	1848:1882	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	0	23	from	composition	109:119	arg1	hens					146:149	LPS-challenged laying hens	124:149	LPS-challenged laying hens	124:149	Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens.
36587451	2	24	theme	LPS-challenged	449:462	arg1	hens					471:474	LPS-challenged laying hens	449:474	LPS-challenged laying hens	449:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	8	25	theme	cell	1281:1284	arg1	density					1286:1292	goblet cell density	1274:1292	goblet cell density	1274:1292	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	7	26	theme	intestinal	1178:1187	arg1	mucosa					1189:1194	intestinal mucosa	1178:1194	intestinal mucosa of LPS-challenged laying hens	1178:1224	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	5	27	theme	LPS	785:787	arg1	challenge					789:797	LPS challenge	785:797	LPS challenge	785:797	After LPS challenge, 8 birds of each group were randomly selected and sampled.
36587451	1	28	theme	well-known	165:174	arg1	Quercetin					152:160	Quercetin	152:160	Quercetin	152:160	Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds.
36587451	1	28	theme	well-known	165:174	arg1	flavonoid					176:184	a well-known flavonoid	163:184	a well-known flavonoid	163:184	Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds.
36587451	7	29	from	activity	1098:1105	arg1	mucosa					1189:1194	intestinal mucosa	1178:1194	intestinal mucosa of LPS-challenged laying hens	1178:1224	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	0	30	theme	gut	94:96	arg1	composition					109:119	gut microbiota composition	94:119	gut microbiota composition in LPS-challenged laying hens	94:149	Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens.
36587451	9	31	theme	LPS-challenged	1450:1463	arg1	layers					1465:1470	LPS-challenged layers	1450:1470	LPS-challenged layers	1450:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	2	32	from	effects	325:331	arg1	community					436:444	gut microbial community	422:444	gut microbial community in LPS-challenged laying hens	422:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	2	32	from	effects	325:331	arg1	functions					408:416	intestinal barrier functions	389:416	intestinal barrier functions	389:416	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	2	32	from	effects	325:331	arg1	responses					378:386	inflammatory responses	365:386	inflammatory responses	365:386	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	10	33	theme	IL-1β	1600:1604	arg1	levels					1579:1584	the increased expression levels	1554:1584	the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa	1554:1635	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	10	33	theme	IL-1β	1600:1604	arg1	<					1589:1589	P < 0.05	1587:1594	P < 0.05	1587:1594	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	8	34	theme	expression	1305:1314	arg1	levels					1316:1321	mucin2 expression levels	1298:1321	mucin2 expression levels (P < 0.05)	1298:1332	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	8	34	theme	expression	1305:1314	arg1	<					1326:1326	P < 0.05	1324:1331	P < 0.05	1324:1331	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	11	35	from	shift	1754:1758	arg1	microenvironment					1767:1782	gut microenvironment	1763:1782	gut microenvironment	1763:1782	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	2	36	theme	gut	422:424	arg1	community					436:444	gut microbial community	422:444	gut microbial community in LPS-challenged laying hens	422:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	0	37	theme	LPS-challenged	124:137	arg1	hens					146:149	LPS-challenged laying hens	124:149	LPS-challenged laying hens	124:149	Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens.
36587451	4	38	theme	LC	697:698	arg1	groups					707:712	LC and LQ groups	697:712	LC and LQ groups	697:712	LQ group was fed with 0.4 mg/kg quercetin and at the end of 12 wk, LC and LQ groups were challenged intraperitoneally with lipopolysaccharide (LPS).
36587451	7	39	theme	glutathione	1141:1151	arg1	peroxidase					1153:1162	glutathione peroxidase	1141:1162	glutathione peroxidase (P < 0.05)	1141:1173	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	7	39	theme	glutathione	1141:1151	arg1	<					1167:1167	P < 0.05	1165:1172	P < 0.05	1165:1172	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	4	40	theme	LQ	704:705	arg1	groups					707:712	LC and LQ groups	697:712	LC and LQ groups	697:712	LQ group was fed with 0.4 mg/kg quercetin and at the end of 12 wk, LC and LQ groups were challenged intraperitoneally with lipopolysaccharide (LPS).
36587451	10	41	theme	P	1587:1587	arg1	levels					1579:1584	the increased expression levels	1554:1584	the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa	1554:1635	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	10	41	theme	P	1587:1587	arg1	<					1589:1589	P < 0.05	1587:1594	P < 0.05	1587:1594	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	7	42	theme	capacity	1128:1135	arg1	activity					1098:1105	the activity	1094:1105	the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens	1094:1224	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	9	43	theme	P	1500:1500	arg1	<					1502:1502	P < 0.05	1500:1507	P < 0.05	1500:1507	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	7	44	theme	total	1110:1114	arg1	capacity					1128:1135	total antioxidant capacity	1110:1135	total antioxidant capacity	1110:1135	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	9	45	from	decline	1359:1365	arg1	expression					1390:1399	the mRNA expression	1381:1399	the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers	1381:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	9	46	from	expression	1390:1399	arg1	mucosa					1440:1445	intestinal mucosa	1429:1445	intestinal mucosa of LPS-challenged layers	1429:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	10	47	theme	quercetin	1703:1711	arg1	supplementation					1713:1727	dietary quercetin supplementation	1695:1727	dietary quercetin supplementation	1695:1727	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	0	48	theme	intestinal	58:67	arg1	functions					69:77	intestinal functions	58:77	intestinal functions	58:77	Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens.
36587451	2	49	theme	quercetin	336:344	arg1	supplementation					346:360	quercetin supplementation	336:360	quercetin supplementation	336:360	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	1	50	theme	intestinal	240:249	arg1	functions					251:259	intestinal functions	240:259	intestinal functions	240:259	Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds.
36587451	2	51	from	functions	408:416	arg1	hens					471:474	LPS-challenged laying hens	449:474	LPS-challenged laying hens	449:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	2	52	from	community	436:444	arg1	hens					471:474	LPS-challenged laying hens	449:474	LPS-challenged laying hens	449:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	2	53	theme	microbial	426:434	arg1	community					436:444	gut microbial community	422:444	gut microbial community in LPS-challenged laying hens	422:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	12	54	theme	dietary	2058:2064	arg1	addition					2076:2083	dietary quercetin addition	2058:2083	dietary quercetin addition	2058:2083	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	2	55	theme	intestinal	389:398	arg1	functions					408:416	intestinal barrier functions	389:416	intestinal barrier functions	389:416	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	7	56	theme	laying	1214:1219	arg1	hens					1221:1224	LPS-challenged laying hens	1199:1224	LPS-challenged laying hens	1199:1224	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	12	57	from	modulation	2211:2220	arg1	microbiota					2229:2238	gut microbiota	2225:2238	gut microbiota	2225:2238	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	3	58	theme	laying	536:541	arg1	hens					543:546	two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens	488:546	two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens	488:546	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	9	59	theme	significant	1347:1357	arg1	decline					1359:1365	a significant decline	1345:1365	a significant decline (P < 0.05)	1345:1376	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	9	59	theme	significant	1347:1357	arg1	<					1370:1370	P < 0.05	1368:1375	P < 0.05	1368:1375	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	6	60	theme	LPS	858:860	arg1	challenge					862:870	LPS challenge	858:870	LPS challenge	858:870	LPS challenge induced an obvious intestinal mucosal injury, necrosis and shedding, while quercetin intervention maintained its structure.
36587451	12	61	theme	bacteria	2297:2304	arg1	population					2268:2277	the increased population	2254:2277	the increased population of SCFA-producing bacteria	2254:2304	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	11	62	theme	fatty	1865:1869	arg1	SCFA					1878:1881	SCFA	1878:1881	SCFA	1878:1881	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	62	theme	fatty	1865:1869	arg1	acids					1871:1875	some short chain fatty acids	1848:1875	some short chain fatty acids (SCFA)	1848:1882	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	2	63	from	responses	378:386	arg1	hens					471:474	LPS-challenged laying hens	449:474	LPS-challenged laying hens	449:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	3	64	theme	CON	588:590	arg1	groups					576:581	3 groups	574:581	3 groups	574:581	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	3	64	theme	CON	588:590	arg1	group					592:596	the CON group	584:596	the CON group	584:596	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	5	65	theme	group	816:820	arg1	birds					802:806	8 birds	800:806	8 birds of each group	800:820	After LPS challenge, 8 birds of each group were randomly selected and sampled.
36587451	4	66	theme	0.4	652:654	arg1	mg/kg					656:660	mg/kg	656:660	mg/kg	656:660	LQ group was fed with 0.4 mg/kg quercetin and at the end of 12 wk, LC and LQ groups were challenged intraperitoneally with lipopolysaccharide (LPS).
36587451	7	67	theme	malondialdehyde	1043:1057	arg1	<					1071:1071	P < 0.05	1069:1076	P < 0.05	1069:1076	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	7	67	theme	malondialdehyde	1043:1057	arg1	contents					1059:1066	the elevated malondialdehyde contents	1030:1066	the elevated malondialdehyde contents (P < 0.05)	1030:1077	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	10	68	dep	reversed	1665:1672	arg1	<					1677:1677	P < 0.05	1675:1682	P < 0.05	1675:1682	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	10	69	theme	LPS	1532:1534	arg1	challenge					1536:1544	LPS challenge	1532:1544	LPS challenge	1532:1544	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	2	70	theme	supplementation	346:360	arg1	effects					325:331	the effects	321:331	the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens	321:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	1	71	from	microbiota	269:278	arg1	birds					283:287	birds	283:287	birds	283:287	Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds.
36587451	3	72	theme	LC	603:604	arg1	groups					576:581	3 groups	574:581	3 groups	574:581	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	3	72	theme	LC	603:604	arg1	group					606:610	the LC group	599:610	the LC group	599:610	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	6	73	theme	mucosal	902:908	arg1	injury					910:915	an obvious intestinal mucosal injury	880:915	an obvious intestinal mucosal injury	880:915	LPS challenge induced an obvious intestinal mucosal injury, necrosis and shedding, while quercetin intervention maintained its structure.
36587451	12	74	theme	SCFA-producing	2282:2295	arg1	bacteria					2297:2304	SCFA-producing bacteria	2282:2304	SCFA-producing bacteria	2282:2304	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	11	75	theme	short	1853:1857	arg1	SCFA					1878:1881	SCFA	1878:1881	SCFA	1878:1881	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	75	theme	short	1853:1857	arg1	acids					1871:1875	some short chain fatty acids	1848:1875	some short chain fatty acids (SCFA)	1848:1882	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	9	76	theme	Claudin1	1404:1411	arg1	expression					1390:1399	the mRNA expression	1381:1399	the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers	1381:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	6	77	theme	obvious	883:889	arg1	injury					910:915	an obvious intestinal mucosal injury	880:915	an obvious intestinal mucosal injury	880:915	LPS challenge induced an obvious intestinal mucosal injury, necrosis and shedding, while quercetin intervention maintained its structure.
36587451	10	78	dep	these	1644:1648	arg1	rises					1650:1654	rises	1650:1654	rises	1650:1654	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	3	79	theme	LQ	620:621	arg1	groups					576:581	3 groups	574:581	3 groups	574:581	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	3	79	theme	LQ	620:621	arg1	group					623:627	the LQ group	616:627	the LQ group	616:627	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	9	80	theme	Occludin	1417:1424	arg1	expression					1390:1399	the mRNA expression	1381:1399	the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers	1381:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	9	81	theme	intestinal	1429:1438	arg1	mucosa					1440:1445	intestinal mucosa	1429:1445	intestinal mucosa of LPS-challenged layers	1429:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	8	82	theme	goblet	1274:1279	arg1	density					1286:1292	goblet cell density	1274:1292	goblet cell density	1274:1292	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	0	83	theme	microbiota	98:107	arg1	composition					109:119	gut microbiota composition	94:119	gut microbiota composition in LPS-challenged laying hens	94:149	Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens.
36587451	0	84	theme	intestinal	21:30	arg1	inflammation					32:43	intestinal inflammation	21:43	intestinal inflammation	21:43	Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens.
36587451	6	85	theme	quercetin	947:955	arg1	intervention					957:968	quercetin intervention	947:968	quercetin intervention	947:968	LPS challenge induced an obvious intestinal mucosal injury, necrosis and shedding, while quercetin intervention maintained its structure.
36587451	12	86	theme	gut	2225:2227	arg1	microbiota					2229:2238	gut microbiota	2225:2238	gut microbiota	2225:2238	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	9	87	dep	alleviated	1488:1497	arg1	<					1502:1502	P < 0.05	1500:1507	P < 0.05	1500:1507	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	7	88	theme	P	1165:1165	arg1	peroxidase					1153:1162	glutathione peroxidase	1141:1162	glutathione peroxidase (P < 0.05)	1141:1173	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	7	88	theme	P	1165:1165	arg1	<					1167:1167	P < 0.05	1165:1172	P < 0.05	1165:1172	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	8	89	from	decreases	1261:1269	arg1	levels					1316:1321	mucin2 expression levels	1298:1321	mucin2 expression levels (P < 0.05)	1298:1332	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	8	89	from	decreases	1261:1269	arg1	<					1326:1326	P < 0.05	1324:1331	P < 0.05	1324:1331	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	8	89	from	decreases	1261:1269	arg1	density					1286:1292	goblet cell density	1274:1292	goblet cell density	1274:1292	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	9	90	theme	layers	1465:1470	arg1	mucosa					1440:1445	intestinal mucosa	1429:1445	intestinal mucosa of LPS-challenged layers	1429:1470	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	11	91	theme	-producing	1883:1892	arg1	Negativicutes					1954:1966	Negativicutes	1954:1966	Negativicutes	1954:1966	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	91	theme	-producing	1883:1892	arg1	bacteria					1914:1921	some short chain fatty acids (SCFA)-producing or health-promoting bacteria	1848:1921	some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis	1848:2040	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	91	theme	-producing	1883:1892	arg1	Prevotellaceae					1997:2010	Prevotellaceae	1997:2010	Prevotellaceae	1997:2010	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	91	theme	-producing	1883:1892	arg1	Bacteroides_salanitronis					2017:2040	Bacteroides_salanitronis	2017:2040	Bacteroides_salanitronis	2017:2040	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	11	91	theme	-producing	1883:1892	arg1	Megamonas					1986:1994	Megamonas	1986:1994	Megamonas	1986:1994	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	10	92	from	levels	1579:1584	arg1	mucosa					1630:1635	intestinal mucosa	1619:1635	intestinal mucosa	1619:1635	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	8	93	theme	mucin2	1298:1303	arg1	levels					1316:1321	mucin2 expression levels	1298:1321	mucin2 expression levels (P < 0.05)	1298:1332	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	8	93	theme	mucin2	1298:1303	arg1	<					1326:1326	P < 0.05	1324:1331	P < 0.05	1324:1331	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	0	94	theme	laying	139:144	arg1	hens					146:149	LPS-challenged laying hens	124:149	LPS-challenged laying hens	124:149	Quercetin alleviates intestinal inflammation and improves intestinal functions via modulating gut microbiota composition in LPS-challenged laying hens.
36587451	1	95	theme	beneficial	218:227	arg1	effects					229:235	beneficial effects	218:235	beneficial effects	218:235	Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds.
36587451	12	96	theme	intestinal	2160:2169	arg1	functions					2171:2179	intestinal functions	2160:2179	intestinal functions	2160:2179	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	7	97	theme	peroxidase	1153:1162	arg1	activity					1098:1105	the activity	1094:1105	the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens	1094:1224	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	8	98	theme	P	1324:1324	arg1	levels					1316:1321	mucin2 expression levels	1298:1321	mucin2 expression levels (P < 0.05)	1298:1332	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	8	98	theme	P	1324:1324	arg1	<					1326:1326	P < 0.05	1324:1331	P < 0.05	1324:1331	Quercetin rescued the LPS-induced decreases in goblet cell density and mucin2 expression levels (P < 0.05).
36587451	2	99	theme	barrier	400:406	arg1	functions					408:416	intestinal barrier functions	389:416	intestinal barrier functions	389:416	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	7	100	theme	antioxidant	1116:1126	arg1	capacity					1128:1135	total antioxidant capacity	1110:1135	total antioxidant capacity	1110:1135	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	11	101	theme	relative	1826:1833	arg1	abundance					1835:1843	the relative abundance	1822:1843	the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis	1822:2040	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	2	102	theme	laying	464:469	arg1	hens					471:474	LPS-challenged laying hens	449:474	LPS-challenged laying hens	449:474	In this study, we investigated the effects of quercetin supplementation on inflammatory responses, intestinal barrier functions and gut microbial community in LPS-challenged laying hens.
36587451	12	103	theme	intestinal	2123:2132	arg1	inflammation					2134:2145	the LPS challenge-induced intestinal inflammation	2097:2145	the LPS challenge-induced intestinal inflammation	2097:2145	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	10	104	theme	increased	1558:1566	arg1	levels					1579:1584	the increased expression levels	1554:1584	the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa	1554:1635	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	10	104	theme	increased	1558:1566	arg1	<					1589:1589	P < 0.05	1587:1594	P < 0.05	1587:1594	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	12	105	theme	quercetin	2066:2074	arg1	addition					2076:2083	dietary quercetin addition	2058:2083	dietary quercetin addition	2058:2083	In conclusion, dietary quercetin addition ameliorated the LPS challenge-induced intestinal inflammation and improved intestinal functions, possibly associated with its modulation on gut microbiota, particularly the increased population of SCFA-producing bacteria.
36587451	10	106	theme	expression	1568:1577	arg1	levels					1579:1584	the increased expression levels	1554:1584	the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa	1554:1635	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	10	106	theme	expression	1568:1577	arg1	<					1589:1589	P < 0.05	1587:1594	P < 0.05	1587:1594	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	3	107	theme	No.6	531:534	arg1	hens					543:546	two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens	488:546	two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens	488:546	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	11	108	theme	LPS	1730:1732	arg1	challenge					1734:1742	LPS challenge	1730:1742	LPS challenge	1730:1742	LPS challenge induced a shift in gut microenvironment, and quercetin addition could elevate the relative abundance of some short chain fatty acids (SCFA)-producing or health-promoting bacteria such as Phascolarctobacterium, Negativicutes, Selenomonadales, Megamonas, Prevotellaceae, and Bacteroides_salanitronis.
36587451	9	109	theme	dietary	1513:1519	arg1	quercetin					1521:1529	dietary quercetin	1513:1529	dietary quercetin	1513:1529	There was a significant decline (P < 0.05) in the mRNA expression of Claudin1 and Occludin in intestinal mucosa of LPS-challenged layers, which could be alleviated (P < 0.05) by dietary quercetin.
36587451	7	110	theme	hens	1221:1224	arg1	mucosa					1189:1194	intestinal mucosa	1178:1194	intestinal mucosa of LPS-challenged laying hens	1178:1224	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	3	111	theme	32-wk-old	513:521	arg1	hens					543:546	two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens	488:546	two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens	488:546	A total of two hundred eighty-eight 32-wk-old Jingfen No.6 laying hens were randomly assigned to 3 groups, the CON group, the LC group and the LQ group.
36587451	7	112	theme	LPS-challenged	1199:1212	arg1	hens					1221:1224	LPS-challenged laying hens	1199:1224	LPS-challenged laying hens	1199:1224	Quercetin significantly decreased the elevated malondialdehyde contents (P < 0.05), and increased the activity of total antioxidant capacity and glutathione peroxidase (P < 0.05) in intestinal mucosa of LPS-challenged laying hens.
36587451	10	113	theme	TLR-4	1610:1614	arg1	levels					1579:1584	the increased expression levels	1554:1584	the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa	1554:1635	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	10	113	theme	TLR-4	1610:1614	arg1	<					1589:1589	P < 0.05	1587:1594	P < 0.05	1587:1594	LPS challenge induced the increased expression levels (P < 0.05) of IL-1β and TLR-4 in intestinal mucosa, while these rises could be reversed (P < 0.05) following dietary quercetin supplementation.
36587451	1	114	theme	gut	265:267	arg1	microbiota					269:278	gut microbiota	265:278	gut microbiota in birds	265:287	Quercetin, a well-known flavonoid, has been demonstrated to exert beneficial effects on intestinal functions and gut microbiota in birds.
36109831	5	0	theme	microbiome	857:866	arg1	composition					814:824	composition	814:824	composition	814:824	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	0	theme	microbiome	857:866	arg1	functionality					830:842	functionality	830:842	functionality	830:842	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	1	from	effects	738:744	arg1	composition					814:824	composition	814:824	composition	814:824	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	1	from	effects	738:744	arg1	functionality					830:842	functionality	830:842	functionality	830:842	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	11	2	theme	increased	1752:1760	arg1	concentrations					1762:1775	increased concentrations	1752:1775	increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1752:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	7	3	theme	descending	1148:1157	arg1	colons					1159:1164	ascending, transverse and descending colons	1122:1164	ascending, transverse and descending colons	1122:1164	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	1	4	theme	docosahexaenoic	262:276	arg1	DHA					284:286	DHA	284:286	DHA	284:286	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	4	theme	docosahexaenoic	262:276	arg1	acid					278:281	docosahexaenoic acid	262:281	docosahexaenoic acid (DHA)	262:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	4	theme	docosahexaenoic	262:276	arg1	acids					212:216	omega-3 polyunsaturated fatty acids	182:216	omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)	182:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	5	5	dep	composition	814:824	arg1	the					810:812	the	810:812	the	810:812	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	10	6	theme	luminal	1725:1731	arg1	habitats					1733:1740	luminal habitats	1725:1740	luminal habitats	1725:1740	The ω-3 PUFAs also induced a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats.
36109831	7	7	theme	transverse	1133:1142	arg1	colons					1159:1164	ascending, transverse and descending colons	1122:1164	ascending, transverse and descending colons	1122:1164	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	0	8	theme	fermentation	146:157	arg1	system					159:164	a human fermentation system	138:164	a human fermentation system	138:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	8	9	theme	ω-3	1277:1279	arg1	supplementation					1286:1300	ω-3 PUFA supplementation	1277:1300	ω-3 PUFA supplementation	1277:1300	We show that ω-3 PUFA supplementation modulates the microbiota in a gut region- and niche-dependent fashion.
36109831	10	10	theme	significant	1657:1667	arg1	depletion					1669:1677	a gradual and significant depletion	1643:1677	a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats	1643:1740	The ω-3 PUFAs also induced a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats.
36109831	11	11	theme	supplementation	1861:1875	arg1	end					1850:1852	the end	1846:1852	the end of the supplementation	1846:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	7	12	theme	ascending	1122:1130	arg1	colons					1159:1164	ascending, transverse and descending colons	1122:1164	ascending, transverse and descending colons	1122:1164	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	1	13	theme	fatty	206:210	arg1	acid					247:250	eicosapentaenoic acid	230:250	eicosapentaenoic acid (EPA)	230:256	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	13	theme	fatty	206:210	arg1	acid					278:281	docosahexaenoic acid	262:281	docosahexaenoic acid (DHA)	262:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	13	theme	fatty	206:210	arg1	acids					212:216	omega-3 polyunsaturated fatty acids	182:216	omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)	182:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	13	theme	fatty	206:210	arg1	PUFAs					223:227	ω-3 PUFAs	219:227	ω-3 PUFAs	219:227	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	11	14	from	regions	1835:1841	arg1	concentrations					1762:1775	increased concentrations	1752:1775	increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1752:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	10	15	theme	gradual	1645:1651	arg1	depletion					1669:1677	a gradual and significant depletion	1643:1677	a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats	1643:1740	The ω-3 PUFAs also induced a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats.
36109831	7	16	theme	ileum	1115:1119	arg1	Luminal					1062:1068	Luminal	1062:1068	Luminal	1062:1068	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	7	16	theme	ileum	1115:1119	arg1	microbiota					1097:1106	outer mucus-associated microbiota	1074:1106	outer mucus-associated microbiota	1074:1106	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	10	17	theme	<i>Clostridia</i>	1696:1712	arg1	members					1714:1720	non-mucolytic <i>Clostridia</i> members	1682:1720	non-mucolytic <i>Clostridia</i> members	1682:1720	The ω-3 PUFAs also induced a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats.
36109831	7	18	theme	fecal	1197:1201	arg1	inoculates					1203:1212	fecal inoculates	1197:1212	fecal inoculates	1197:1212	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	5	19	theme	Mucosal	873:879	arg1	Simulator					881:889	a Mucosal Simulator	871:889	a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®)	871:944	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	7	20	theme	mucus-associated	1080:1095	arg1	microbiota					1097:1106	outer mucus-associated microbiota	1074:1106	outer mucus-associated microbiota	1074:1106	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	6	21	theme	microbial	951:959	arg1	communities					961:971	Gut microbial communities	947:971	Gut microbial communities derived from one individual harvested in two different seasons	947:1034	Gut microbial communities derived from one individual harvested in two different seasons were tested in duplicate.
36109831	5	22	theme	ω-3	778:780	arg1	supplementation					791:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	23	theme	Microbial	915:923	arg1	Ecosystem					925:933	the Human Intestinal Microbial Ecosystem	894:933	the Human Intestinal Microbial Ecosystem (M-SHIME®)	894:944	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	23	theme	Microbial	915:923	arg1	M-SHIME®					936:943	M-SHIME®	936:943	M-SHIME®	936:943	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	24	theme	supplementation	791:805	arg1	effects					738:744	the effects	734:744	the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome	734:866	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	9	25	theme	<i>Firmicutes</i>-mucolytic	1578:1604	arg1	bacteria					1606:1613	<i>Firmicutes</i>-mucolytic bacteria	1578:1613	<i>Firmicutes</i>-mucolytic bacteria	1578:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	3	26	from	effects	572:578	arg1	microbiome					591:600	the gut microbiome	583:600	the gut microbiome	583:600	However, these prebiotic properties have been poorly investigated and direct effects on the gut microbiome have never been explored dynamically across gut regions and niches (lumen <i>vs</i>.
36109831	5	27	theme	1 week	749:754	arg1	supplementation					791:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	7	28	from	inoculates	1203:1212	arg1	28 d					1187:1190	28 d	1187:1190	28 d from fecal inoculates	1187:1212	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	9	29	theme	ω-3	1475:1477	arg1	PUFAs					1479:1483	ω-3 PUFAs	1475:1483	ω-3 PUFAs	1475:1483	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	2	30	theme	mucin	446:450	arg1	muciniphila</i>					478:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	0	31	theme	mucolytic	84:92	arg1	species					94:100	mucolytic species	84:100	mucolytic species from the gut luminal mucin niche in a human fermentation system	84:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	2	32	theme	gut	379:381	arg1	microbiota					383:392	the gut microbiota	375:392	the gut microbiota	375:392	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	0	33	theme	Short-term	0:9	arg1	supplementation					11:25	Short-term supplementation	0:25	Short-term supplementation with ω-3 polyunsaturated fatty acids	0:62	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	11	34	theme	chain	1790:1794	arg1	acids					1802:1806	short chain fatty acids	1784:1806	the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1780:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	34	theme	chain	1790:1794	arg1	SCFA					1809:1812	SCFA	1809:1812	SCFA	1809:1812	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	5	35	theme	human	851:855	arg1	microbiome					857:866	the human microbiome	847:866	the human microbiome	847:866	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	9	36	theme	outer	1377:1381	arg1	microbiota					1400:1409	The outer mucus-associated microbiota	1373:1409	The outer mucus-associated microbiota	1373:1409	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	3	37	theme	lumen	670:674	arg1	<i>vs</i>					676:684	lumen <i>vs</i>	670:684	lumen <i>vs</i>	670:684	However, these prebiotic properties have been poorly investigated and direct effects on the gut microbiome have never been explored dynamically across gut regions and niches (lumen <i>vs</i>.
36109831	2	38	theme	health-promoting	407:422	arg1	muciniphila</i>					478:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	2	38	theme	health-promoting	407:422	arg1	bacteria					424:431	health-promoting bacteria	407:431	health-promoting bacteria	407:431	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	0	39	theme	polyunsaturated	36:50	arg1	acids					58:62	ω-3 polyunsaturated fatty acids	32:62	ω-3 polyunsaturated fatty acids	32:62	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	2	40	theme	<i>Akkermansia	463:476	arg1	muciniphila</i>					478:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	11	41	theme	<i>Akkermansia	1921:1934	arg1	muciniphila</i>					1936:1950	<i>Akkermansia muciniphila</i>	1921:1950	<i>Akkermansia muciniphila</i>	1921:1950	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	1	42	theme	health	311:316	arg1	benefits					318:325	multifaceted health benefits	298:325	multifaceted health benefits	298:325	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	2	43	theme	Recent	328:333	arg1	studies					335:341	Recent studies	328:341	Recent studies	328:341	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	11	44	theme	acids	1802:1806	arg1	propionate					1815:1824	the short chain fatty acids (SCFA) propionate	1780:1824	the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1780:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	1	45	dep	acids	212:216	arg1	acid					247:250	eicosapentaenoic acid	230:250	eicosapentaenoic acid (EPA)	230:256	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	45	dep	acids	212:216	arg1	DHA					284:286	DHA	284:286	DHA	284:286	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	45	dep	acids	212:216	arg1	acid					278:281	docosahexaenoic acid	262:281	docosahexaenoic acid (DHA)	262:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	45	dep	acids	212:216	arg1	acids					212:216	omega-3 polyunsaturated fatty acids	182:216	omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)	182:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	45	dep	acids	212:216	arg1	EPA					253:255	EPA	253:255	EPA	253:255	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	6	46	theme	Gut	947:949	arg1	communities					961:971	Gut microbial communities	947:971	Gut microbial communities derived from one individual harvested in two different seasons	947:1034	Gut microbial communities derived from one individual harvested in two different seasons were tested in duplicate.
36109831	0	47	from	niche	129:133	arg1	species					94:100	mucolytic species	84:100	mucolytic species from the gut luminal mucin niche in a human fermentation system	84:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	0	47	from	niche	129:133	arg1	system					159:164	a human fermentation system	138:164	a human fermentation system	138:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	2	48	theme	ω-3	356:358	arg1	PUFAs					360:364	ω-3 PUFAs	356:364	ω-3 PUFAs	356:364	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	9	49	theme	mucin	1458:1462	arg1	habitat					1464:1470	the luminal mucin habitat	1446:1470	the luminal mucin habitat	1446:1470	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	10	50	from	depletion	1669:1677	arg1	habitats					1733:1740	luminal habitats	1725:1740	luminal habitats	1725:1740	The ω-3 PUFAs also induced a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats.
36109831	3	51	theme	direct	565:570	arg1	effects					572:578	direct effects	565:578	direct effects on the gut microbiome	565:600	However, these prebiotic properties have been poorly investigated and direct effects on the gut microbiome have never been explored dynamically across gut regions and niches (lumen <i>vs</i>.
36109831	9	52	from	opposition	1550:1559	arg1	blooming					1504:1511	a remarkable blooming	1491:1511	a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria	1491:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	7	53	theme	last	1254:1257	arg1	7 d					1259:1261	the last 7 d	1250:1261	the last 7 d	1250:1261	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	3	54	theme	gut	587:589	arg1	microbiome					591:600	the gut microbiome	583:600	the gut microbiome	583:600	However, these prebiotic properties have been poorly investigated and direct effects on the gut microbiome have never been explored dynamically across gut regions and niches (lumen <i>vs</i>.
36109831	1	55	theme	polyunsaturated	190:204	arg1	acid					247:250	eicosapentaenoic acid	230:250	eicosapentaenoic acid (EPA)	230:256	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	55	theme	polyunsaturated	190:204	arg1	acid					278:281	docosahexaenoic acid	262:281	docosahexaenoic acid (DHA)	262:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	55	theme	polyunsaturated	190:204	arg1	acids					212:216	omega-3 polyunsaturated fatty acids	182:216	omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)	182:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	55	theme	polyunsaturated	190:204	arg1	PUFAs					223:227	ω-3 PUFAs	219:227	ω-3 PUFAs	219:227	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	9	56	from	muciniphila</i>	1531:1545	arg1	opposition					1550:1559	opposition	1550:1559	opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria	1550:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	4	57	theme	mucus-associated	687:702	arg1	microbiota					704:713	mucus-associated microbiota	687:713	mucus-associated microbiota	687:713	mucus-associated microbiota).
36109831	9	58	theme	remarkable	1493:1502	arg1	blooming					1504:1511	a remarkable blooming	1491:1511	a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria	1491:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	8	59	theme	PUFA	1281:1284	arg1	supplementation					1286:1300	ω-3 PUFA supplementation	1277:1300	ω-3 PUFA supplementation	1277:1300	We show that ω-3 PUFA supplementation modulates the microbiota in a gut region- and niche-dependent fashion.
36109831	10	60	theme	members	1714:1720	arg1	depletion					1669:1677	a gradual and significant depletion	1643:1677	a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats	1643:1740	The ω-3 PUFAs also induced a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats.
36109831	0	61	theme	human	140:144	arg1	system					159:164	a human fermentation system	138:164	a human fermentation system	138:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	6	62	attach	derived	973:979	arg1	individual					990:999	one individual	986:999	one individual harvested in two different seasons	986:1034	Gut microbial communities derived from one individual harvested in two different seasons were tested in duplicate.
36109831	6	62	attach	derived	973:979	arg2	communities					961:971	Gut microbial communities	947:971	Gut microbial communities derived from one individual harvested in two different seasons	947:1034	Gut microbial communities derived from one individual harvested in two different seasons were tested in duplicate.
36109831	5	63	theme	Human	898:902	arg1	Ecosystem					925:933	the Human Intestinal Microbial Ecosystem	894:933	the Human Intestinal Microbial Ecosystem (M-SHIME®)	894:944	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	63	theme	Human	898:902	arg1	M-SHIME®					936:943	M-SHIME®	936:943	M-SHIME®	936:943	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	1	64	theme	ω-3	219:221	arg1	acids					212:216	omega-3 polyunsaturated fatty acids	182:216	omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)	182:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	64	theme	ω-3	219:221	arg1	PUFAs					223:227	ω-3 PUFAs	219:227	ω-3 PUFAs	219:227	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	11	65	from	end	1850:1852	arg1	propionate					1815:1824	the short chain fatty acids (SCFA) propionate	1780:1824	the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1780:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	65	from	end	1850:1852	arg1	regions					1835:1841	colon regions	1829:1841	colon regions at the end of the supplementation	1829:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	10	66	theme	non-mucolytic	1682:1694	arg1	members					1714:1720	non-mucolytic <i>Clostridia</i> members	1682:1720	non-mucolytic <i>Clostridia</i> members	1682:1720	The ω-3 PUFAs also induced a gradual and significant depletion of non-mucolytic <i>Clostridia</i> members in luminal habitats.
36109831	7	67	theme	outer	1074:1078	arg1	microbiota					1097:1106	outer mucus-associated microbiota	1074:1106	outer mucus-associated microbiota	1074:1106	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	9	68	theme	muciniphila</i>	1531:1545	arg1	blooming					1504:1511	a remarkable blooming	1491:1511	a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria	1491:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	11	69	from	propionate	1815:1824	arg1	regions					1835:1841	colon regions	1829:1841	colon regions at the end of the supplementation	1829:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	69	from	propionate	1815:1824	arg1	end					1850:1852	the end	1846:1852	the end of the supplementation	1846:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	3	70	theme	gut	646:648	arg1	regions					650:656	gut regions	646:656	gut regions	646:656	However, these prebiotic properties have been poorly investigated and direct effects on the gut microbiome have never been explored dynamically across gut regions and niches (lumen <i>vs</i>.
36109831	11	71	theme	colon	1829:1833	arg1	regions					1835:1841	colon regions	1829:1841	colon regions at the end of the supplementation	1829:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	5	72	theme	DHA-enriched	765:776	arg1	supplementation					791:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	1	73	theme	acids	212:216	arg1	Consumption					167:177	Consumption	167:177	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)	167:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	5	74	theme	Intestinal	904:913	arg1	Ecosystem					925:933	the Human Intestinal Microbial Ecosystem	894:933	the Human Intestinal Microbial Ecosystem (M-SHIME®)	894:944	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	74	theme	Intestinal	904:913	arg1	M-SHIME®					936:943	M-SHIME®	936:943	M-SHIME®	936:943	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	8	75	theme	region-	1336:1342	arg1	fashion					1364:1370	a gut region- and niche-dependent fashion	1330:1370	a gut region- and niche-dependent fashion	1330:1370	We show that ω-3 PUFA supplementation modulates the microbiota in a gut region- and niche-dependent fashion.
36109831	11	76	theme	propionate	1815:1824	arg1	concentrations					1762:1775	increased concentrations	1752:1775	increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1752:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	5	77	theme	fish-oil	782:789	arg1	supplementation					791:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	5	78	theme	Ecosystem	925:933	arg1	Simulator					881:889	a Mucosal Simulator	871:889	a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®)	871:944	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	8	79	theme	niche-dependent	1348:1362	arg1	fashion					1364:1370	a gut region- and niche-dependent fashion	1330:1370	a gut region- and niche-dependent fashion	1330:1370	We show that ω-3 PUFA supplementation modulates the microbiota in a gut region- and niche-dependent fashion.
36109831	0	80	theme	gut	111:113	arg1	niche					129:133	the gut luminal mucin niche	107:133	the gut luminal mucin niche in a human fermentation system	107:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	9	81	theme	bacteria	1606:1613	arg1	decrease					1566:1573	a decrease	1564:1573	a decrease of <i>Firmicutes</i>-mucolytic bacteria	1564:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	6	82	theme	different	1018:1026	arg1	seasons					1028:1034	two different seasons	1014:1034	two different seasons	1014:1034	Gut microbial communities derived from one individual harvested in two different seasons were tested in duplicate.
36109831	7	83	theme	ω-3	1236:1238	arg1	PUFAs					1240:1244	ω-3 PUFAs	1236:1244	ω-3 PUFAs for the last 7 d	1236:1261	Luminal and outer mucus-associated microbiota of the ileum, ascending, transverse and descending colons were cultivated over 28 d from fecal inoculates and supplemented with ω-3 PUFAs for the last 7 d.
36109831	0	84	theme	mucin	123:127	arg1	niche					129:133	the gut luminal mucin niche	107:133	the gut luminal mucin niche in a human fermentation system	107:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	5	85	theme	EPA-	756:759	arg1	supplementation					791:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	1 week EPA- and DHA-enriched ω-3 fish-oil supplementation	749:805	Thus, we studied the effects of 1 week EPA- and DHA-enriched ω-3 fish-oil supplementation on the composition and functionality of the human microbiome in a Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME®).
36109831	11	86	theme	<i>Desulfovibrionia</i>	1971:1993	arg1	class					1995:1999	the <i>Desulfovibrionia</i> class	1967:1999	the <i>Desulfovibrionia</i> class	1967:1999	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	87	theme	class	1995:1999	arg1	members					1956:1962	members	1956:1962	members of the <i>Desulfovibrionia</i> class	1956:1999	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	87	theme	class	1995:1999	arg1	muciniphila</i>					1936:1950	<i>Akkermansia muciniphila</i>	1921:1950	<i>Akkermansia muciniphila</i>	1921:1950	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	0	88	theme	ω-3	32:34	arg1	acids					58:62	ω-3 polyunsaturated fatty acids	32:62	ω-3 polyunsaturated fatty acids	32:62	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	11	89	from	concentrations	1762:1775	arg1	regions					1835:1841	colon regions	1829:1841	colon regions at the end of the supplementation	1829:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	90	theme	short	1784:1788	arg1	acids					1802:1806	short chain fatty acids	1784:1806	the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1780:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	90	theme	short	1784:1788	arg1	SCFA					1809:1812	SCFA	1809:1812	SCFA	1809:1812	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	0	91	theme	fatty	52:56	arg1	acids					58:62	ω-3 polyunsaturated fatty acids	32:62	ω-3 polyunsaturated fatty acids	32:62	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	9	92	theme	mucus-associated	1383:1398	arg1	microbiota					1400:1409	The outer mucus-associated microbiota	1373:1409	The outer mucus-associated microbiota	1373:1409	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	0	93	theme	luminal	115:121	arg1	niche					129:133	the gut luminal mucin niche	107:133	the gut luminal mucin niche in a human fermentation system	107:164	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	1	94	theme	multifaceted	298:309	arg1	benefits					318:325	multifaceted health benefits	298:325	multifaceted health benefits	298:325	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	2	95	theme	specialist	452:461	arg1	muciniphila</i>					478:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	the mucin specialist <i>Akkermansia muciniphila</i>	442:492	Recent studies suggest that ω-3 PUFAs modulate the gut microbiota by enhancing health-promoting bacteria, such as the mucin specialist <i>Akkermansia muciniphila</i>.
36109831	0	96	with	supplementation	11:25	arg1	acids					58:62	ω-3 polyunsaturated fatty acids	32:62	ω-3 polyunsaturated fatty acids	32:62	Short-term supplementation with ω-3 polyunsaturated fatty acids modulates primarily mucolytic species from the gut luminal mucin niche in a human fermentation system.
36109831	3	97	theme	prebiotic	510:518	arg1	properties					520:529	these prebiotic properties	504:529	these prebiotic properties	504:529	However, these prebiotic properties have been poorly investigated and direct effects on the gut microbiome have never been explored dynamically across gut regions and niches (lumen <i>vs</i>.
36109831	9	98	theme	higher	1423:1428	arg1	resilience					1430:1439	a higher resilience	1421:1439	a higher resilience than the luminal mucin habitat to ω-3 PUFAs	1421:1483	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	9	99	from	blooming	1504:1511	arg1	opposition					1550:1559	opposition	1550:1559	opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria	1550:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	11	100	theme	fatty	1796:1800	arg1	acids					1802:1806	short chain fatty acids	1784:1806	the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation	1780:1875	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	11	100	theme	fatty	1796:1800	arg1	SCFA					1809:1812	SCFA	1809:1812	SCFA	1809:1812	Finally, increased concentrations of the short chain fatty acids (SCFA) propionate in colon regions at the end of the supplementation was associated positively with the bloom of <i>Akkermansia muciniphila</i> and members of the <i>Desulfovibrionia</i> class.
36109831	1	101	theme	eicosapentaenoic	230:245	arg1	acid					247:250	eicosapentaenoic acid	230:250	eicosapentaenoic acid (EPA)	230:256	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	101	theme	eicosapentaenoic	230:245	arg1	acids					212:216	omega-3 polyunsaturated fatty acids	182:216	omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)	182:287	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	1	101	theme	eicosapentaenoic	230:245	arg1	EPA					253:255	EPA	253:255	EPA	253:255	Consumption of omega-3 polyunsaturated fatty acids (ω-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) provides multifaceted health benefits.
36109831	9	102	theme	<i>Akkermansia	1516:1529	arg1	muciniphila</i>					1531:1545	<i>Akkermansia muciniphila</i>	1516:1545	<i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria	1516:1613	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
36109831	9	103	theme	luminal	1450:1456	arg1	habitat					1464:1470	the luminal mucin habitat	1446:1470	the luminal mucin habitat	1446:1470	The outer mucus-associated microbiota displayed a higher resilience than the luminal mucin habitat to ω-3 PUFAs, with a remarkable blooming of <i>Akkermansia muciniphila</i> in opposition to a decrease of <i>Firmicutes</i>-mucolytic bacteria.
35730951	6	0	theme	-induced	904:911	arg1	model					927:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	or their metabolites in a dextran sodium sulfate (DSS)-induced colitis mouse model.
35730951	17	1	theme	superior	2349:2356	arg1	effect					2358:2363	a superior effect	2347:2363	a superior effect	2347:2363	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	18	2	theme	therapeutic	2598:2608	arg1	approach					2610:2617	an alternative therapeutic approach	2583:2617	an alternative therapeutic approach to treat IBD	2583:2630	These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
35730951	18	2	theme	therapeutic	2598:2608	arg1	utilization					2545:2555	the utilization	2541:2555	the utilization of probiotic consortia	2541:2578	These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
35730951	10	3	theme	probiotic	1381:1389	arg1	consortia					1391:1399	probiotic consortia	1381:1399	probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score	1381:1517	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	2	4	theme	potential	254:262	arg1	association					264:274	a potential association	252:274	a potential association between the gut microbiota and inflammatory signatures	252:329	Although the pathogenesis of the disease is unknown, a potential association between the gut microbiota and inflammatory signatures has been established.
35730951	4	5	theme	comprehensive	637:649	arg1	effects					651:657	the comprehensive effects	633:657	the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus	633:776	In the current study, we investigated the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus (Lactobacillus spp.)
35730951	7	6	dep	composition	1043:1053	arg1	the					1039:1041	the	1039:1041	the	1039:1041	Our data demonstrate that probiotic consortia not only ameliorate the disease phenotype but also restore the composition and structure of the gut microbiota.
35730951	8	7	theme	probiotic	1170:1178	arg1	strain					1180:1185	any single probiotic strain	1159:1185	any single probiotic strain	1159:1185	Moreover, the effect of probiotic consortia is better than that of any single probiotic strain.
35730951	17	8	theme	control	2460:2466	arg1	group					2468:2472	the control group	2456:2472	the control group	2456:2472	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	7	9	theme	microbiota	1080:1089	arg1	structure					1059:1067	structure	1059:1067	structure	1059:1067	Our data demonstrate that probiotic consortia not only ameliorate the disease phenotype but also restore the composition and structure of the gut microbiota.
35730951	7	9	theme	microbiota	1080:1089	arg1	composition					1043:1053	composition	1043:1053	composition	1043:1053	Our data demonstrate that probiotic consortia not only ameliorate the disease phenotype but also restore the composition and structure of the gut microbiota.
35730951	11	10	theme	mixed	1533:1537	arg1	metabolites					1539:1549	mixed metabolites	1533:1549	mixed metabolites	1533:1549	In addition, mixed metabolites led only to changes in intestinal flora composition.
35730951	2	11	theme	gut	288:290	arg1	microbiota					292:301	the gut microbiota and inflammatory signatures	284:329	microbiota	292:301	Although the pathogenesis of the disease is unknown, a potential association between the gut microbiota and inflammatory signatures has been established.
35730951	1	12	theme	global	169:174	arg1	problem					190:196	a global public health problem	167:196	a global public health problem	167:196	Inflammatory bowel disease (IBD) has become a global public health problem.
35730951	17	13	located	observed	2444:2451	arg2	effects					2436:2442	the effects	2432:2442	the effects observed in the control group or groups administered with a single strain	2432:2516	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	17	13	located	observed	2444:2451	arg1	groups					2477:2482	groups	2477:2482	groups administered with a single strain	2477:2516	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	17	13	located	observed	2444:2451	arg1	group					2468:2472	the control group	2456:2472	the control group	2456:2472	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	11	14	from	changes	1563:1569	arg1	composition					1591:1601	intestinal flora composition	1574:1601	intestinal flora composition	1574:1601	In addition, mixed metabolites led only to changes in intestinal flora composition.
35730951	9	15	theme	fermentation	1228:1239	arg1	metabolites					1241:1251	mixed fermentation metabolites	1222:1251	mixed fermentation metabolites	1222:1251	The results also demonstrate that mixed fermentation metabolites are capable of ameliorating the symptoms of gut inflammation.
35730951	18	16	theme	consortia	2570:2578	arg1	utilization					2545:2555	the utilization	2541:2555	the utilization of probiotic consortia	2541:2578	These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
35730951	18	16	theme	consortia	2570:2578	arg1	approach					2610:2617	an alternative therapeutic approach	2583:2617	an alternative therapeutic approach to treat IBD	2583:2630	These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
35730951	12	17	theme	colitis	1700:1706	arg1	model					1714:1718	the DSS-induced colitis mouse model	1684:1718	the DSS-induced colitis mouse model	1684:1718	In summary, probiotic consortia and metabolites could exert protective roles in the DSS-induced colitis mouse model by reducing inflammation and regulating microbial dysbiosis.
35730951	6	18	theme	mouse	921:925	arg1	model					927:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	or their metabolites in a dextran sodium sulfate (DSS)-induced colitis mouse model.
35730951	16	19	from	efficacy	2202:2209	arg1	recovery					2232:2239	the recovery	2228:2239	the recovery of gut inflammation	2228:2259	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	16	19	from	efficacy	2202:2209	arg1	restoration					2269:2279	the restoration	2265:2279	the restoration of gut microecology	2265:2299	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	1	20	theme	bowel	136:140	arg1	IBD					151:153	IBD	151:153	IBD	151:153	Inflammatory bowel disease (IBD) has become a global public health problem.
35730951	1	20	theme	bowel	136:140	arg1	disease					142:148	Inflammatory bowel disease	123:148	Inflammatory bowel disease (IBD)	123:154	Inflammatory bowel disease (IBD) has become a global public health problem.
35730951	4	21	theme	bacterial	672:680	arg1	consortia					682:690	probiotic bacterial consortia	662:690	probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus	662:776	In the current study, we investigated the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus (Lactobacillus spp.)
35730951	16	22	theme	gut	2284:2286	arg1	microecology					2288:2299	gut microecology	2284:2299	gut microecology	2284:2299	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	10	23	theme	body	1453:1456	arg1	weight					1458:1463	body weight	1453:1463	body weight	1453:1463	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	13	24	theme	alternative	1899:1909	arg1	strategy					1923:1930	an alternative therapeutic strategy	1896:1930	an alternative therapeutic strategy for IBD	1896:1938	These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD.
35730951	0	25	theme	Bowel	82:86	arg1	Diseases					88:95	Inflammatory Bowel Diseases	69:95	Inflammatory Bowel Diseases	69:95	Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model.
35730951	9	26	theme	inflammation	1301:1312	arg1	symptoms					1285:1292	the symptoms	1281:1292	the symptoms of gut inflammation	1281:1312	The results also demonstrate that mixed fermentation metabolites are capable of ameliorating the symptoms of gut inflammation.
35730951	10	27	theme	activity	1474:1481	arg1	DAI					1490:1492	DAI	1490:1492	DAI	1490:1492	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	10	27	theme	activity	1474:1481	arg1	index					1483:1487	disease activity index	1466:1487	disease activity index (DAI)	1466:1493	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	13	28	theme	microbial	1874:1882	arg1	products					1884:1891	probiotic-based microbial products	1858:1891	probiotic-based microbial products as an alternative therapeutic strategy for IBD	1858:1938	These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD.
35730951	3	29	theme	food	430:433	arg1	supplements					435:445	food supplements	430:445	food supplements	430:445	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	3	29	theme	food	430:433	arg1	Probiotics					353:362	Probiotics	353:362	Probiotics	353:362	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	0	30	theme	Colitis	102:108	arg1	Model					116:120	a Colitis Mouse Model	100:120	a Colitis Mouse Model	100:120	Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model.
35730951	15	31	theme	entire	2072:2077	arg1	colon					2079:2083	the entire colon	2068:2083	the entire colon	2068:2083	IBD is characterized by a wide range of lesions, often involving the entire colon, and is characterized mainly by ulcers and erosions of the colonic mucosa.
35730951	0	32	theme	Probiotic	0:8	arg1	Consortia					10:18	Probiotic Consortia	0:18	Probiotic Consortia	0:18	Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model.
35730951	16	33	theme	gut	2244:2246	arg1	inflammation					2248:2259	gut inflammation	2244:2259	gut inflammation	2244:2259	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	5	34	theme	Bifidobacterium	828:842	arg1	spp					844:846	Bifidobacterium spp	828:846	Bifidobacterium spp.	828:847	, and Bifidobacterium lactis (Bifidobacterium spp.)
35730951	11	35	theme	intestinal	1574:1583	arg1	composition					1591:1601	intestinal flora composition	1574:1601	intestinal flora composition	1574:1601	In addition, mixed metabolites led only to changes in intestinal flora composition.
35730951	14	36	theme	nonspecific	1969:1979	arg1	IBD					1952:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	14	36	theme	nonspecific	1969:1979	arg1	disease					1994:2000	a chronic nonspecific inflammatory disease	1959:2000	a chronic nonspecific inflammatory disease	1959:2000	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	2	37	theme	inflammatory	307:318	arg1	signatures					320:329	the gut microbiota and inflammatory signatures	284:329	signatures	320:329	Although the pathogenesis of the disease is unknown, a potential association between the gut microbiota and inflammatory signatures has been established.
35730951	17	38	theme	single	2504:2509	arg1	strain					2511:2516	a single strain	2502:2516	a single strain	2502:2516	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	13	39	theme	current	1805:1811	arg1	study					1813:1817	the current study	1801:1817	the current study	1801:1817	These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD.
35730951	8	40	theme	probiotic	1116:1124	arg1	consortia					1126:1134	probiotic consortia	1116:1134	probiotic consortia	1116:1134	Moreover, the effect of probiotic consortia is better than that of any single probiotic strain.
35730951	6	41	theme	colitis	913:919	arg1	model					927:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	or their metabolites in a dextran sodium sulfate (DSS)-induced colitis mouse model.
35730951	18	42	theme	probiotic	2560:2568	arg1	consortia					2570:2578	probiotic consortia	2560:2578	probiotic consortia	2560:2578	These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
35730951	3	43	theme	gastrointestinal	490:505	arg1	disorders					507:515	gastrointestinal disorders	490:515	gastrointestinal disorders	490:515	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	12	44	theme	microbial	1760:1768	arg1	dysbiosis					1770:1778	microbial dysbiosis	1760:1778	microbial dysbiosis	1760:1778	In summary, probiotic consortia and metabolites could exert protective roles in the DSS-induced colitis mouse model by reducing inflammation and regulating microbial dysbiosis.
35730951	1	45	theme	health	183:188	arg1	problem					190:196	a global public health problem	167:196	a global public health problem	167:196	Inflammatory bowel disease (IBD) has become a global public health problem.
35730951	5	46	dep	lactis	820:825	arg1	spp					844:846	Bifidobacterium spp	828:846	Bifidobacterium spp.	828:847	, and Bifidobacterium lactis (Bifidobacterium spp.)
35730951	16	47	theme	probiotics	2214:2223	arg1	efficacy					2202:2209	the efficacy	2198:2209	the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology	2198:2299	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	3	48	with	patients	469:476	arg1	IBD					483:485	IBD	483:485	IBD	483:485	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	3	48	with	patients	469:476	arg1	disorders					507:515	gastrointestinal disorders	490:515	gastrointestinal disorders	490:515	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	14	49	theme	IMPORTANCE	1941:1950	arg1	IBD					1952:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	14	49	theme	IMPORTANCE	1941:1950	arg1	disease					1994:2000	a chronic nonspecific inflammatory disease	1959:2000	a chronic nonspecific inflammatory disease	1959:2000	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	6	50	from	metabolites	859:869	arg1	model					927:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	a dextran sodium sulfate (DSS)-induced colitis mouse model	874:931	or their metabolites in a dextran sodium sulfate (DSS)-induced colitis mouse model.
35730951	7	51	theme	gut	1076:1078	arg1	microbiota					1080:1089	the gut microbiota	1072:1089	the gut microbiota	1072:1089	Our data demonstrate that probiotic consortia not only ameliorate the disease phenotype but also restore the composition and structure of the gut microbiota.
35730951	10	52	theme	phenotypic	1417:1426	arg1	index					1483:1487	disease activity index	1466:1487	disease activity index (DAI)	1466:1493	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	10	52	theme	phenotypic	1417:1426	arg1	score					1513:1517	histological score	1500:1517	histological score	1500:1517	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	10	52	theme	phenotypic	1417:1426	arg1	weight					1458:1463	body weight	1453:1463	body weight	1453:1463	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	10	52	theme	phenotypic	1417:1426	arg1	characteristics					1428:1442	phenotypic characteristics	1417:1442	phenotypic characteristics	1417:1442	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	8	53	theme	single	1163:1168	arg1	strain					1180:1185	any single probiotic strain	1159:1185	any single probiotic strain	1159:1185	Moreover, the effect of probiotic consortia is better than that of any single probiotic strain.
35730951	1	54	theme	public	176:181	arg1	problem					190:196	a global public health problem	167:196	a global public health problem	167:196	Inflammatory bowel disease (IBD) has become a global public health problem.
35730951	18	55	theme	alternative	2586:2596	arg1	approach					2610:2617	an alternative therapeutic approach	2583:2617	an alternative therapeutic approach to treat IBD	2583:2630	These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
35730951	18	55	theme	alternative	2586:2596	arg1	utilization					2545:2555	the utilization	2541:2555	the utilization of probiotic consortia	2541:2578	These results support the utilization of probiotic consortia as an alternative therapeutic approach to treat IBD.
35730951	9	56	theme	mixed	1222:1226	arg1	metabolites					1241:1251	mixed fermentation metabolites	1222:1251	mixed fermentation metabolites	1222:1251	The results also demonstrate that mixed fermentation metabolites are capable of ameliorating the symptoms of gut inflammation.
35730951	3	57	theme	gut	573:575	arg1	microenvironment					577:592	the gut microenvironment	569:592	the gut microenvironment	569:592	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	16	58	theme	present	2167:2173	arg1	study					2175:2179	the present study	2163:2179	the present study	2163:2179	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	7	59	theme	disease	1004:1010	arg1	phenotype					1012:1020	the disease phenotype	1000:1020	the disease phenotype	1000:1020	Our data demonstrate that probiotic consortia not only ameliorate the disease phenotype but also restore the composition and structure of the gut microbiota.
35730951	1	60	theme	Inflammatory	123:134	arg1	IBD					151:153	IBD	151:153	IBD	151:153	Inflammatory bowel disease (IBD) has become a global public health problem.
35730951	1	60	theme	Inflammatory	123:134	arg1	disease					142:148	Inflammatory bowel disease	123:148	Inflammatory bowel disease (IBD)	123:154	Inflammatory bowel disease (IBD) has become a global public health problem.
35730951	12	61	theme	DSS-induced	1688:1698	arg1	model					1714:1718	the DSS-induced colitis mouse model	1684:1718	the DSS-induced colitis mouse model	1684:1718	In summary, probiotic consortia and metabolites could exert protective roles in the DSS-induced colitis mouse model by reducing inflammation and regulating microbial dysbiosis.
35730951	16	62	theme	microecology	2288:2299	arg1	recovery					2232:2239	the recovery	2228:2239	the recovery of gut inflammation	2228:2259	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	16	62	theme	microecology	2288:2299	arg1	restoration					2269:2279	the restoration	2265:2279	the restoration of gut microecology	2265:2299	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	0	63	theme	Inflammatory	69:80	arg1	Diseases					88:95	Inflammatory Bowel Diseases	69:95	Inflammatory Bowel Diseases	69:95	Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model.
35730951	13	64	from	study	1813:1817	arg1	findings					1787:1794	These findings	1781:1794	These findings from the current study	1781:1817	These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD.
35730951	2	65	theme	disease	232:238	arg1	pathogenesis					212:223	the pathogenesis	208:223	the pathogenesis of the disease	208:238	Although the pathogenesis of the disease is unknown, a potential association between the gut microbiota and inflammatory signatures has been established.
35730951	2	65	theme	disease	232:238	arg1	unknown					243:249	unknown	243:249	unknown	243:249	Although the pathogenesis of the disease is unknown, a potential association between the gut microbiota and inflammatory signatures has been established.
35730951	15	66	theme	colonic	2144:2150	arg1	mucosa					2152:2157	the colonic mucosa	2140:2157	the colonic mucosa	2140:2157	IBD is characterized by a wide range of lesions, often involving the entire colon, and is characterized mainly by ulcers and erosions of the colonic mucosa.
35730951	12	67	theme	mouse	1708:1712	arg1	model					1714:1718	the DSS-induced colitis mouse model	1684:1718	the DSS-induced colitis mouse model	1684:1718	In summary, probiotic consortia and metabolites could exert protective roles in the DSS-induced colitis mouse model by reducing inflammation and regulating microbial dysbiosis.
35730951	4	68	theme	probiotic	662:670	arg1	consortia					682:690	probiotic bacterial consortia	662:690	probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus	662:776	In the current study, we investigated the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus (Lactobacillus spp.)
35730951	0	69	theme	Diseases	88:95	arg1	Symptoms					57:64	the Symptoms	53:64	the Symptoms of Inflammatory Bowel Diseases	53:95	Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model.
35730951	12	70	theme	protective	1664:1673	arg1	roles					1675:1679	protective roles	1664:1679	protective roles	1664:1679	In summary, probiotic consortia and metabolites could exert protective roles in the DSS-induced colitis mouse model by reducing inflammation and regulating microbial dysbiosis.
35730951	4	71	theme	consortia	682:690	arg1	effects					651:657	the comprehensive effects	633:657	the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus	633:776	In the current study, we investigated the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus (Lactobacillus spp.)
35730951	13	72	theme	therapeutic	1911:1921	arg1	strategy					1923:1930	an alternative therapeutic strategy	1896:1930	an alternative therapeutic strategy for IBD	1896:1938	These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD.
35730951	9	73	theme	gut	1297:1299	arg1	inflammation					1301:1312	gut inflammation	1297:1312	gut inflammation	1297:1312	The results also demonstrate that mixed fermentation metabolites are capable of ameliorating the symptoms of gut inflammation.
35730951	7	74	theme	probiotic	960:968	arg1	consortia					970:978	probiotic consortia	960:978	probiotic consortia	960:978	Our data demonstrate that probiotic consortia not only ameliorate the disease phenotype but also restore the composition and structure of the gut microbiota.
35730951	13	75	theme	probiotic-based	1858:1872	arg1	products					1884:1891	probiotic-based microbial products	1858:1891	probiotic-based microbial products as an alternative therapeutic strategy for IBD	1858:1938	These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD.
35730951	0	76	theme	Mouse	110:114	arg1	Model					116:120	a Colitis Mouse Model	100:120	a Colitis Mouse Model	100:120	Probiotic Consortia and Their Metabolites Ameliorate the Symptoms of Inflammatory Bowel Diseases in a Colitis Mouse Model.
35730951	15	77	theme	mucosa	2152:2157	arg1	ulcers					2117:2122	ulcers	2117:2122	ulcers	2117:2122	IBD is characterized by a wide range of lesions, often involving the entire colon, and is characterized mainly by ulcers and erosions of the colonic mucosa.
35730951	15	77	theme	mucosa	2152:2157	arg1	erosions					2128:2135	erosions	2128:2135	erosions	2128:2135	IBD is characterized by a wide range of lesions, often involving the entire colon, and is characterized mainly by ulcers and erosions of the colonic mucosa.
35730951	10	78	theme	disease	1466:1472	arg1	DAI					1490:1492	DAI	1490:1492	DAI	1490:1492	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	10	78	theme	disease	1466:1472	arg1	index					1483:1487	disease activity index	1466:1487	disease activity index (DAI)	1466:1493	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	13	79	theme	products	1884:1891	arg1	development					1843:1853	the development	1839:1853	the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD	1839:1938	These findings from the current study provide support for the development of probiotic-based microbial products as an alternative therapeutic strategy for IBD.
35730951	16	80	theme	inflammation	2248:2259	arg1	recovery					2232:2239	the recovery	2228:2239	the recovery of gut inflammation	2228:2259	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	16	80	theme	inflammation	2248:2259	arg1	restoration					2269:2279	the restoration	2265:2279	the restoration of gut microecology	2265:2299	In the present study, we investigated the efficacy of probiotics on the recovery of gut inflammation and the restoration of gut microecology.
35730951	15	81	theme	lesions	2043:2049	arg1	range					2034:2038	a wide range	2027:2038	a wide range of lesions, often involving the entire colon,	2027:2084	IBD is characterized by a wide range of lesions, often involving the entire colon, and is characterized mainly by ulcers and erosions of the colonic mucosa.
35730951	4	82	dep	investigated	620:631	arg1	spp					793:795	spp	793:795	spp	793:795	In the current study, we investigated the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus (Lactobacillus spp.)
35730951	12	83	theme	probiotic	1616:1624	arg1	consortia					1626:1634	probiotic consortia	1616:1634	probiotic consortia	1616:1634	In summary, probiotic consortia and metabolites could exert protective roles in the DSS-induced colitis mouse model by reducing inflammation and regulating microbial dysbiosis.
35730951	15	84	theme	wide	2029:2032	arg1	range					2034:2038	a wide range	2027:2038	a wide range of lesions, often involving the entire colon,	2027:2084	IBD is characterized by a wide range of lesions, often involving the entire colon, and is characterized mainly by ulcers and erosions of the colonic mucosa.
35730951	17	85	contain	have	2342:2345	arg2	effect					2358:2363	a superior effect	2347:2363	a superior effect	2347:2363	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	17	85	contain	have	2342:2345	arg1	consortia					2332:2340	probiotic consortia	2322:2340	probiotic consortia	2322:2340	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	10	86	theme	metabolites	1346:1356	arg1	administration					1328:1341	the administration	1324:1341	the administration of metabolites	1324:1356	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	10	86	theme	metabolites	1346:1356	arg1	effective					1368:1376	effective	1368:1376	effective	1368:1376	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	3	87	theme	microbial	450:458	arg1	drugs					460:464	microbial drugs	450:464	microbial drugs	450:464	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	3	87	theme	microbial	450:458	arg1	Probiotics					353:362	Probiotics	353:362	Probiotics	353:362	Probiotics, especially Lactobacillus or Bifidobacterium, are orally taken as food supplements or microbial drugs by patients with IBD or gastrointestinal disorders due to their safety, efficacy, and power to restore the gut microenvironment.
35730951	14	88	theme	inflammatory	1981:1992	arg1	IBD					1952:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	14	88	theme	inflammatory	1981:1992	arg1	disease					1994:2000	a chronic nonspecific inflammatory disease	1959:2000	a chronic nonspecific inflammatory disease	1959:2000	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	10	89	theme	histological	1500:1511	arg1	score					1513:1517	histological score	1500:1517	histological score	1500:1517	However, the administration of metabolites is not as effective as probiotic consortia with respect to phenotypic characteristics, such as body weight, disease activity index (DAI), and histological score.
35730951	14	90	theme	chronic	1961:1967	arg1	IBD					1952:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD	1941:1954	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	14	90	theme	chronic	1961:1967	arg1	disease					1994:2000	a chronic nonspecific inflammatory disease	1959:2000	a chronic nonspecific inflammatory disease	1959:2000	IMPORTANCE IBD is a chronic nonspecific inflammatory disease.
35730951	17	91	theme	probiotic	2322:2330	arg1	consortia					2332:2340	probiotic consortia	2322:2340	probiotic consortia	2322:2340	We demonstrate that probiotic consortia have a superior effect in inhibiting inflammation and accelerating recovery compared with the effects observed in the control group or groups administered with a single strain.
35730951	8	92	theme	consortia	1126:1134	arg1	better					1139:1144	better	1139:1144	better	1139:1144	Moreover, the effect of probiotic consortia is better than that of any single probiotic strain.
35730951	8	92	theme	consortia	1126:1134	arg1	effect					1106:1111	the effect	1102:1111	the effect of probiotic consortia	1102:1134	Moreover, the effect of probiotic consortia is better than that of any single probiotic strain.
35730951	4	93	theme	current	602:608	arg1	study					610:614	the current study	598:614	the current study	598:614	In the current study, we investigated the comprehensive effects of probiotic bacterial consortia consisting of Lactobacillus reuteri, Lactobacillus gasseri, Lactobacillus acidophilus (Lactobacillus spp.)
35730951	11	94	theme	flora	1585:1589	arg1	composition					1591:1601	intestinal flora composition	1574:1601	intestinal flora composition	1574:1601	In addition, mixed metabolites led only to changes in intestinal flora composition.
36145974	4	0	theme	efficient	1014:1022	arg1	neutralization					1038:1051	efficient sheet surface neutralization	1014:1051	efficient sheet surface neutralization	1014:1051	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	6	1	theme	chemical	1250:1257	arg1	constitution					1259:1270	The chemical constitution	1246:1270	The chemical constitution of the materials	1246:1287	The chemical constitution of the materials was monitored by FTIR.
36145974	5	2	theme	amorphous	1175:1183	arg1	nature					1185:1190	their amorphous nature	1169:1190	their amorphous nature	1169:1190	For each sheet, their surface morphologies were observed by Field-emission microscopy, and DSC was used to confirm their amorphous nature and the impact of the introduction of every additive.
36145974	4	3	theme	surface	1030:1036	arg1	neutralization					1038:1051	efficient sheet surface neutralization	1014:1051	efficient sheet surface neutralization	1014:1051	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	3	4	theme	wt	722:723	arg1	additives					737:745	various additives	729:745	various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents	729:846	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	4	theme	wt	722:723	arg1	chloride					821:828	25 wt% sodium chloride	807:828	25 wt% sodium chloride	807:828	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	4	theme	wt	722:723	arg1	polysaccharides					763:777	natural polysaccharides	755:777	natural polysaccharides	755:777	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	4	theme	wt	722:723	arg1	polymers					790:797	synthetic polymers	780:797	synthetic polymers	780:797	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	4	theme	wt	722:723	arg1	%					724:724	4 wt%	720:724	4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents	720:846	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	7	5	theme	candidates	1382:1391	arg1	candidates					1382:1391	our most promising candidates	1363:1391	our most promising candidates	1363:1391	Then, cytotoxicity tests were performed for six of our most promising candidates.
36145974	7	5	theme	candidates	1382:1391	arg1	six					1356:1358	six	1356:1358	six	1356:1358	Then, cytotoxicity tests were performed for six of our most promising candidates.
36145974	7	6	theme	most	1367:1370	arg1	candidates					1382:1391	our most promising candidates	1363:1391	our most promising candidates	1363:1391	Then, cytotoxicity tests were performed for six of our most promising candidates.
36145974	3	7	theme	additives	737:745	arg1	additives					737:745	various additives	729:745	various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents	729:846	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	7	theme	additives	737:745	arg1	chloride					821:828	25 wt% sodium chloride	807:828	25 wt% sodium chloride	807:828	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	7	theme	additives	737:745	arg1	polysaccharides					763:777	natural polysaccharides	755:777	natural polysaccharides	755:777	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	7	theme	additives	737:745	arg1	polymers					790:797	synthetic polymers	780:797	synthetic polymers	780:797	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	7	theme	additives	737:745	arg1	%					724:724	4 wt%	720:724	4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents	720:846	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	1	8	theme	new	201:203	arg1	elastomers					238:247	new potent, bio-based, biocompatible elastomers	201:247	new potent, bio-based, biocompatible elastomers	201:247	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	4	9	theme	oligomers	900:908	arg1	removal					853:859	The removal	849:859	The removal of unreacted monomers and acidic short oligomers	849:908	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	0	10	theme	Elastomer	142:150	arg1	Composites					152:161	Biocompatible and Electroconductive Elastomer Composites	106:161	Biocompatible and Electroconductive Elastomer Composites	106:161	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	1	11	theme	sebacic	402:408	arg1	acid					410:413	sebacic acid	402:413	sebacic acid	402:413	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	2	12	theme	exploitable	582:592	arg1	prepolymers					594:604	all exploitable prepolymers	578:604	all exploitable prepolymers	578:604	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	6	13	theme	materials	1279:1287	arg1	constitution					1259:1270	The chemical constitution	1246:1270	The chemical constitution of the materials	1246:1287	The chemical constitution of the materials was monitored by FTIR.
36145974	3	14	theme	porogen	833:839	arg1	agents					841:846	porogen agents	833:846	porogen agents	833:846	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	4	15	theme	acidic	887:892	arg1	oligomers					900:908	acidic short oligomers	887:908	acidic short oligomers	887:908	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	7	16	theme	cytotoxicity	1318:1329	arg1	tests					1331:1335	cytotoxicity tests	1318:1335	cytotoxicity tests	1318:1335	Then, cytotoxicity tests were performed for six of our most promising candidates.
36145974	4	17	dep	washing	938:944	arg1	means					929:933	means	929:933	means	929:933	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	1	18	theme	2,5-furandicarboxylic	420:440	arg1	acid					442:445	2,5-furandicarboxylic acid	420:445	2,5-furandicarboxylic acid	420:445	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	9	19	theme	materials	1712:1720	arg1	effectiveness					1689:1701	the effectiveness	1685:1701	the effectiveness of these materials as performant, time-dependent electric pH sensors	1685:1770	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	8	20	theme	different	1437:1445	arg1	types					1447:1451	two different types	1433:1451	two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively	1433:1643	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	3	21	theme	synthetic	780:788	arg1	polymers					790:797	synthetic polymers	780:797	synthetic polymers	780:797	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	2	22	theme	melt	486:489	arg1	polycondensation					491:506	modified melt polycondensation	477:506	modified melt polycondensation	477:506	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	3	23	theme	%	812:812	arg1	chloride					821:828	25 wt% sodium chloride	807:828	25 wt% sodium chloride	807:828	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	0	24	theme	Sheets	76:81	arg1	Extrusion					27:35	Extrusion	27:35	Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites	27:161	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	1	25	theme	growing	175:181	arg1	demand					190:195	growing global demand	175:195	growing global demand for new potent, bio-based, biocompatible elastomers	175:247	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	8	26	theme	PGS	1471:1473	arg1	elastomers					1475:1484	extrusion-made PGS elastomers	1456:1484	extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively	1456:1643	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	2	27	theme	sheets	662:667	arg1	manufacturing					635:647	the easy and rapid manufacturing	616:647	the easy and rapid manufacturing of elastomer sheets by extrusion	616:680	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	5	28	theme	additive	1236:1243	arg1	introduction					1214:1225	the introduction	1210:1225	the introduction of every additive	1210:1243	For each sheet, their surface morphologies were observed by Field-emission microscopy, and DSC was used to confirm their amorphous nature and the impact of the introduction of every additive.
36145974	1	29	theme	sheets	362:367	arg1	production					293:302	the solvent-free production	276:302	the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol	276:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	9	30	theme	pH	1761:1762	arg1	sensors					1764:1770	performant, time-dependent electric pH sensors	1725:1770	performant, time-dependent electric pH sensors	1725:1770	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	5	31	theme	surface	1076:1082	arg1	morphologies					1084:1095	their surface morphologies	1070:1095	their surface morphologies	1070:1095	For each sheet, their surface morphologies were observed by Field-emission microscopy, and DSC was used to confirm their amorphous nature and the impact of the introduction of every additive.
36145974	0	32	theme	Solvent-Free	0:11	arg1	Production					13:22	Solvent-Free Production	0:22	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.	0:162	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	1	33	theme	solvent-free	280:291	arg1	production					293:302	the solvent-free production	276:302	the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol	276:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	8	34	theme	%	1503:1503	arg1	particulates					1510:1521	10 wt% PANI particulates	1498:1521	10 wt% PANI particulates	1498:1521	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	9	35	dep	performant	1725:1734	arg1	time-dependent					1737:1750	time-dependent	1737:1750	time-dependent	1737:1750	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	1	36	with	acid	396:399	arg1	glycerol					459:466	glycerol	459:466	glycerol	459:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	9	37	theme	persistent	1791:1800	arg1	atmosphere					1806:1815	a persistent HCl atmosphere	1789:1815	a persistent HCl atmosphere	1789:1815	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	1	38	theme	pure	310:313	arg1	production					293:302	the solvent-free production	276:302	the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol	276:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	4	39	theme	pH	980:981	arg1	monitoring					983:992	pH monitoring	980:992	pH monitoring	980:992	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	0	40	theme	Poly	50:53	arg1	Sheets					76:81	Bio-Based Poly(glycerol-co-diacids) Sheets	40:81	Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites	40:161	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	1	41	theme	derived	319:325	arg1	sheets					362:367	derived poly(glycerol-co-diacid) composite sheets	319:367	derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol	319:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	8	42	theme	%	1531:1531	arg1	cellulose					1550:1558	4 wt% microcrystalline cellulose	1527:1558	4 wt% microcrystalline cellulose	1527:1558	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	1	43	dep	potent	205:210	arg1	biocompatible					224:236	biocompatible	224:236	biocompatible	224:236	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	1	43	dep	potent	205:210	arg1	bio-based					213:221	bio-based	213:221	bio-based	213:221	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	4	44	theme	sheet	1024:1028	arg1	neutralization					1038:1051	efficient sheet surface neutralization	1014:1051	efficient sheet surface neutralization	1014:1051	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	4	45	theme	aqueous	958:964	arg1	solution					966:973	NaHCO3 aqueous solution	951:973	NaHCO3 aqueous solution	951:973	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	1	46	theme	present	254:260	arg1	study					262:266	the present study	250:266	the present study	250:266	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	1	47	theme	global	183:188	arg1	demand					190:195	growing global demand	175:195	growing global demand for new potent, bio-based, biocompatible elastomers	175:247	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	2	48	theme	rapid	629:633	arg1	manufacturing					635:647	the easy and rapid manufacturing	616:647	the easy and rapid manufacturing of elastomer sheets by extrusion	616:680	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	2	49	theme	modified	477:484	arg1	polycondensation					491:506	modified melt polycondensation	477:506	modified melt polycondensation	477:506	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	2	50	theme	easy	620:623	arg1	manufacturing					635:647	the easy and rapid manufacturing	616:647	the easy and rapid manufacturing of elastomer sheets by extrusion	616:680	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	7	51	theme	promising	1372:1380	arg1	candidates					1382:1391	our most promising candidates	1363:1391	our most promising candidates	1363:1391	Then, cytotoxicity tests were performed for six of our most promising candidates.
36145974	0	52	theme	Electroconductive	124:140	arg1	Composites					152:161	Biocompatible and Electroconductive Elastomer Composites	106:161	Biocompatible and Electroconductive Elastomer Composites	106:161	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	1	53	theme	potent	205:210	arg1	elastomers					238:247	new potent, bio-based, biocompatible elastomers	201:247	new potent, bio-based, biocompatible elastomers	201:247	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	4	54	theme	short	894:898	arg1	oligomers					900:908	acidic short oligomers	887:908	acidic short oligomers	887:908	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	3	55	theme	various	729:735	arg1	additives					737:745	various additives	729:745	various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents	729:846	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	55	theme	various	729:735	arg1	chloride					821:828	25 wt% sodium chloride	807:828	25 wt% sodium chloride	807:828	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	55	theme	various	729:735	arg1	polysaccharides					763:777	natural polysaccharides	755:777	natural polysaccharides	755:777	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	3	55	theme	various	729:735	arg1	polymers					790:797	synthetic polymers	780:797	synthetic polymers	780:797	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	0	56	theme	Composites	152:161	arg1	Development					91:101	the Development	87:101	the Development of Biocompatible and Electroconductive Elastomer Composites	87:161	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	9	57	theme	preliminary	1651:1661	arg1	experiment					1663:1672	a preliminary experiment	1649:1672	a preliminary experiment	1649:1672	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	2	58	theme	-catalyzed	518:527	arg1	polytransesterification					529:551	Co(II)-catalyzed polytransesterification	512:551	Co(II)-catalyzed polytransesterification	512:551	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	5	59	theme	Field-emission	1114:1127	arg1	microscopy					1129:1138	Field-emission microscopy	1114:1138	Field-emission microscopy	1114:1138	For each sheet, their surface morphologies were observed by Field-emission microscopy, and DSC was used to confirm their amorphous nature and the impact of the introduction of every additive.
36145974	4	60	theme	unreacted	864:872	arg1	monomers					874:881	unreacted monomers	864:881	unreacted monomers	864:881	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	3	61	theme	natural	755:761	arg1	polysaccharides					763:777	natural polysaccharides	755:777	natural polysaccharides	755:777	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	8	62	theme	types	1447:1451	arg1	production					1419:1428	the production	1415:1428	the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively	1415:1643	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	8	63	theme	extrusion-made	1456:1469	arg1	elastomers					1475:1484	extrusion-made PGS elastomers	1456:1484	extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively	1456:1643	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	5	64	theme	introduction	1214:1225	arg1	nature					1185:1190	their amorphous nature	1169:1190	their amorphous nature	1169:1190	For each sheet, their surface morphologies were observed by Field-emission microscopy, and DSC was used to confirm their amorphous nature and the impact of the introduction of every additive.
36145974	5	64	theme	introduction	1214:1225	arg1	impact					1200:1205	the impact	1196:1205	the impact of the introduction of every additive	1196:1243	For each sheet, their surface morphologies were observed by Field-emission microscopy, and DSC was used to confirm their amorphous nature and the impact of the introduction of every additive.
36145974	4	65	theme	NaHCO3	951:956	arg1	solution					966:973	NaHCO3 aqueous solution	951:973	NaHCO3 aqueous solution	951:973	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	8	66	theme	elastomers	1475:1484	arg1	types					1447:1451	two different types	1433:1451	two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively	1433:1643	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	3	67	theme	sodium	814:819	arg1	chloride					821:828	25 wt% sodium chloride	807:828	25 wt% sodium chloride	807:828	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	1	68	theme	composite	352:360	arg1	sheets					362:367	derived poly(glycerol-co-diacid) composite sheets	319:367	derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol	319:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	9	69	theme	electric	1752:1759	arg1	sensors					1764:1770	performant, time-dependent electric pH sensors	1725:1770	performant, time-dependent electric pH sensors	1725:1770	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	2	70	theme	elastomer	652:660	arg1	sheets					662:667	elastomer sheets	652:667	elastomer sheets	652:667	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	3	71	theme	wt	810:811	arg1	chloride					821:828	25 wt% sodium chloride	807:828	25 wt% sodium chloride	807:828	Most of our samples were loaded with 4 wt% of various additives such as natural polysaccharides, synthetic polymers, and/or 25 wt% sodium chloride as porogen agents.
36145974	0	72	theme	Biocompatible	106:118	arg1	Composites					152:161	Biocompatible and Electroconductive Elastomer Composites	106:161	Biocompatible and Electroconductive Elastomer Composites	106:161	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	1	73	with	acid	442:445	arg1	glycerol					459:466	glycerol	459:466	glycerol	459:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	2	74	theme	Co	512:513	arg1	polytransesterification					529:551	Co(II)-catalyzed polytransesterification	512:551	Co(II)-catalyzed polytransesterification	512:551	Herein, modified melt polycondensation and Co(II)-catalyzed polytransesterification were employed to produce all exploitable prepolymers, enabling the easy and rapid manufacturing of elastomer sheets by extrusion.
36145974	8	75	theme	wt	1501:1502	arg1	particulates					1510:1521	10 wt% PANI particulates	1498:1521	10 wt% PANI particulates	1498:1521	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	1	76	theme	itaconic	387:394	arg1	acid					396:399	itaconic acid	387:399	itaconic acid	387:399	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	1	76	theme	itaconic	387:394	arg1	FDCA					448:451	FDCA	448:451	FDCA	448:451	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	8	77	theme	PANI	1505:1508	arg1	particulates					1510:1521	10 wt% PANI particulates	1498:1521	10 wt% PANI particulates	1498:1521	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	1	78	with	acid	410:413	arg1	glycerol					459:466	glycerol	459:466	glycerol	459:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	0	79	theme	Bio-Based	40:48	arg1	glycerol-co-diacids					55:73	glycerol-co-diacids	55:73	glycerol-co-diacids	55:73	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	0	79	theme	Bio-Based	40:48	arg1	Poly					50:53	Bio-Based Poly	40:53	Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites	40:161	Solvent-Free Production by Extrusion of Bio-Based Poly(glycerol-co-diacids) Sheets for the Development of Biocompatible and Electroconductive Elastomer Composites.
36145974	9	80	theme	HCl	1802:1804	arg1	atmosphere					1806:1815	a persistent HCl atmosphere	1789:1815	a persistent HCl atmosphere	1789:1815	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	8	81	theme	wt	1529:1530	arg1	cellulose					1550:1558	4 wt% microcrystalline cellulose	1527:1558	4 wt% microcrystalline cellulose	1527:1558	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	1	82	link	derived	319:325	arg1	sheets					362:367	derived poly(glycerol-co-diacid) composite sheets	319:367	derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol	319:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	4	83	theme	monomers	874:881	arg1	removal					853:859	The removal	849:859	The removal of unreacted monomers and acidic short oligomers	849:908	The removal of unreacted monomers and acidic short oligomers was carried out by means of washing with NaHCO3 aqueous solution, and pH monitoring was conducted until efficient sheet surface neutralization.
36145974	8	84	theme	microcrystalline	1533:1548	arg1	cellulose					1550:1558	4 wt% microcrystalline cellulose	1527:1558	4 wt% microcrystalline cellulose	1527:1558	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36145974	1	85	theme	poly	327:330	arg1	sheets					362:367	derived poly(glycerol-co-diacid) composite sheets	319:367	derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol	319:466	Faced with growing global demand for new potent, bio-based, biocompatible elastomers, the present study reports the solvent-free production of 13 pure and derived poly(glycerol-co-diacid) composite sheets exclusively using itaconic acid, sebacic acid, and 2,5-furandicarboxylic acid (FDCA) with glycerol.
36145974	9	86	theme	performant	1725:1734	arg1	sensors					1764:1770	performant, time-dependent electric pH sensors	1725:1770	performant, time-dependent electric pH sensors	1725:1770	In a preliminary experiment, we showed the effectiveness of these materials as performant, time-dependent electric pH sensors when immersed in a persistent HCl atmosphere.
36145974	5	87	used	used	1153:1156	arg2	DSC					1145:1147	DSC	1145:1147	DSC	1145:1147	For each sheet, their surface morphologies were observed by Field-emission microscopy, and DSC was used to confirm their amorphous nature and the impact of the introduction of every additive.
36145974	8	88	theme	potential	1571:1579	arg1	electroconductivity					1581:1599	potential electroconductivity	1571:1599	potential electroconductivity	1571:1599	Finally, we achieved the production of two different types of extrusion-made PGS elastomers loaded with 10 wt% PANI particulates and 4 wt% microcrystalline cellulose for adding potential electroconductivity and stability to the material, respectively.
36982622	0	0	theme	Intravenous	95:105	arg1	Immunoglobulins					107:121	Different Intravenous Immunoglobulins	85:121	Different Intravenous Immunoglobulins for Alzheimer's Disease	85:145	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	6	1	theme	hyperphosphorylated	892:910	arg1	p-tau					917:921	p-tau	917:921	p-tau	917:921	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	6	1	theme	hyperphosphorylated	892:910	arg1	tau					912:914	hyperphosphorylated tau	892:914	hyperphosphorylated tau (p-tau)	892:922	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	0	2	theme	Different	85:93	arg1	Immunoglobulins					107:121	Different Intravenous Immunoglobulins	85:121	Different Intravenous Immunoglobulins for Alzheimer's Disease	85:145	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	5	3	from	differences	773:783	arg1	effects					800:806	therapeutic effects	788:806	therapeutic effects	788:806	In order to investigate the relationship between the composition and function of IVIG and its efficacy in treating AD, we selected three IVIGs that showed notable differences in therapeutic effects.
36982622	9	4	theme	therapeutic	1660:1670	arg1	effect					1672:1677	the therapeutic effect	1656:1677	the therapeutic effect of AD treatment	1656:1693	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	5	5	theme	therapeutic	788:798	arg1	effects					800:806	therapeutic effects	788:806	therapeutic effects	788:806	In order to investigate the relationship between the composition and function of IVIG and its efficacy in treating AD, we selected three IVIGs that showed notable differences in therapeutic effects.
36982622	7	6	theme	anti-Aβ42/tau	1143:1155	arg1	concentration					1166:1178	anti-Aβ42/tau antibody concentration	1143:1178	anti-Aβ42/tau antibody concentration	1143:1178	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	4	7	theme	previous	506:513	arg1	study					515:519	Our previous study	502:519	Our previous study	502:519	Our previous study found that different IVIGs had significantly varied therapeutic effects on 3xTg-AD mice.
36982622	9	8	theme	antibody	1516:1523	arg1	analysis					1525:1532	AD-related antibody analysis	1505:1532	AD-related antibody analysis	1505:1532	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	0	9	theme	Immunoglobulins	107:121	arg1	Potential					72:80	Therapeutic Potential	60:80	Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease	60:145	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	8	10	theme	ability	1496:1502	arg1	level					1443:1447	its level	1439:1447	its level of AD-related antibodies and anti-inflammatory ability	1439:1502	Combined with our previous results, the efficacy of IVIG against AD may be positively correlated with its level of AD-related antibodies and anti-inflammatory ability.
36982622	6	11	from	inflammation	977:988	arg1	concentrations					819:832	the concentrations	815:832	the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice,	815:1040	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	4	12	theme	3xTg-AD	596:602	arg1	mice					604:607	3xTg-AD mice	596:607	3xTg-AD mice	596:607	Our previous study found that different IVIGs had significantly varied therapeutic effects on 3xTg-AD mice.
36982622	5	13	theme	notable	765:771	arg1	differences					773:783	notable differences	765:783	notable differences in therapeutic effects	765:806	In order to investigate the relationship between the composition and function of IVIG and its efficacy in treating AD, we selected three IVIGs that showed notable differences in therapeutic effects.
36982622	8	14	theme	anti-inflammatory	1478:1494	arg1	ability					1496:1502	anti-inflammatory ability	1478:1502	anti-inflammatory ability	1478:1502	Combined with our previous results, the efficacy of IVIG against AD may be positively correlated with its level of AD-related antibodies and anti-inflammatory ability.
36982622	3	15	theme	trials	432:437	arg1	inconsistent					488:499	inconsistent	488:499	inconsistent	488:499	However, the efficacy of clinical trials involving AD patients treated with IVIG has been inconsistent.
36982622	3	15	theme	trials	432:437	arg1	efficacy					411:418	the efficacy	407:418	the efficacy of clinical trials involving AD patients treated with IVIG	407:477	However, the efficacy of clinical trials involving AD patients treated with IVIG has been inconsistent.
36982622	6	16	from	lipopolysaccharide	1001:1018	arg1	mice					1036:1039	Balb/c mice	1029:1039	Balb/c mice	1029:1039	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	9	17	theme	functional	1538:1547	arg1	evaluation					1549:1558	functional evaluation	1538:1558	functional evaluation	1538:1558	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	6	18	theme	Balb/c	1029:1034	arg1	mice					1036:1039	Balb/c mice	1029:1039	Balb/c mice	1029:1039	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	4	19	theme	varied	566:571	arg1	effects					585:591	significantly varied therapeutic effects	552:591	significantly varied therapeutic effects	552:591	Our previous study found that different IVIGs had significantly varied therapeutic effects on 3xTg-AD mice.
36982622	6	20	from	effects	957:963	arg1	inflammation					977:988	systemic inflammation	968:988	systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice	968:1039	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	7	21	from	inflammation	1241:1252	arg1	mice					1312:1315	Balb/c mice	1305:1315	Balb/c mice	1305:1315	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	1	22	theme	common	178:183	arg1	disease					160:166	Alzheimer's disease	148:166	Alzheimer's disease (AD)	148:171	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	1	22	theme	common	178:183	arg1	disease					203:209	a common neurodegenerative disease	176:209	a common neurodegenerative disease that currently has no known cure	176:242	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	0	23	theme	Antibody	0:7	arg1	Assay					9:13	Antibody Assay	0:13	Antibody Assay	0:13	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	6	24	theme	antibodies	846:855	arg1	concentrations					819:832	the concentrations	815:832	the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice,	815:1040	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	3	25	theme	clinical	423:430	arg1	trials					432:437	clinical trials	423:437	clinical trials involving AD patients treated with IVIG	423:477	However, the efficacy of clinical trials involving AD patients treated with IVIG has been inconsistent.
36982622	0	26	theme	Anti-Inflammatory	19:35	arg1	Evaluation					46:55	Anti-Inflammatory Function Evaluation	19:55	Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease	19:145	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	6	27	from	IVIGs	933:937	arg1	inflammation					977:988	systemic inflammation	968:988	systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice	968:1039	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	4	28	contain	had	548:550	arg2	effects					585:591	significantly varied therapeutic effects	552:591	significantly varied therapeutic effects	552:591	Our previous study found that different IVIGs had significantly varied therapeutic effects on 3xTg-AD mice.
36982622	4	28	contain	had	548:550	arg1	IVIGs					542:546	different IVIGs	532:546	different IVIGs	532:546	Our previous study found that different IVIGs had significantly varied therapeutic effects on 3xTg-AD mice.
36982622	5	29	dep	composition	663:673	arg1	the					659:661	the	659:661	the	659:661	In order to investigate the relationship between the composition and function of IVIG and its efficacy in treating AD, we selected three IVIGs that showed notable differences in therapeutic effects.
36982622	4	30	theme	therapeutic	573:583	arg1	effects					585:591	significantly varied therapeutic effects	552:591	significantly varied therapeutic effects	552:591	Our previous study found that different IVIGs had significantly varied therapeutic effects on 3xTg-AD mice.
36982622	9	31	theme	AD	1682:1683	arg1	treatment					1685:1693	AD treatment	1682:1693	AD treatment	1682:1693	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	2	32	theme	AD-related	295:304	arg1	antibodies					306:315	AD-related antibodies	295:315	AD-related antibodies	295:315	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	9	33	theme	AD-related	1505:1514	arg1	analysis					1525:1532	AD-related antibody analysis	1505:1532	AD-related antibody analysis	1505:1532	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	2	34	contain	contains	286:293	arg1	immunoglobulin					257:270	Intravenous immunoglobulin	245:270	Intravenous immunoglobulin (IVIG)	245:277	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	2	34	contain	contains	286:293	arg2	antibodies					306:315	AD-related antibodies	295:315	AD-related antibodies	295:315	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	2	34	contain	contains	286:293	arg1	IVIG					273:276	IVIG	273:276	IVIG	273:276	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	9	35	theme	treatment	1685:1693	arg1	effect					1672:1677	the therapeutic effect	1656:1677	the therapeutic effect of AD treatment	1656:1693	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	1	36	theme	neurodegenerative	185:201	arg1	disease					160:166	Alzheimer's disease	148:166	Alzheimer's disease (AD)	148:171	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	1	36	theme	neurodegenerative	185:201	arg1	disease					203:209	a common neurodegenerative disease	176:209	a common neurodegenerative disease that currently has no known cure	176:242	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	3	37	theme	AD	449:450	arg1	patients					452:459	AD patients	449:459	AD patients treated with IVIG	449:477	However, the efficacy of clinical trials involving AD patients treated with IVIG has been inconsistent.
36982622	0	38	theme	Function	37:44	arg1	Evaluation					46:55	Anti-Inflammatory Function Evaluation	19:55	Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease	19:145	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	6	39	from	concentrations	819:832	arg1	IVIGs					933:937	three IVIGs	927:937	three IVIGs	927:937	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	6	39	from	concentrations	819:832	arg1	effects					957:963	their effects	951:963	their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice	951:1039	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	6	39	from	concentrations	819:832	arg1	inflammation					977:988	systemic inflammation	968:988	systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice	968:1039	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	8	40	theme	previous	1355:1362	arg1	results					1364:1370	our previous results	1351:1370	our previous results	1351:1370	Combined with our previous results, the efficacy of IVIG against AD may be positively correlated with its level of AD-related antibodies and anti-inflammatory ability.
36982622	4	41	theme	different	532:540	arg1	IVIGs					542:546	different IVIGs	532:546	different IVIGs	532:546	Our previous study found that different IVIGs had significantly varied therapeutic effects on 3xTg-AD mice.
36982622	0	42	theme	Therapeutic	60:70	arg1	Potential					72:80	Therapeutic Potential	60:80	Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease	60:145	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	9	43	theme	sufficient	1584:1593	arg1	attention					1595:1603	sufficient attention	1584:1603	sufficient attention	1584:1603	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	8	44	theme	antibodies	1463:1472	arg1	level					1443:1447	its level	1439:1447	its level of AD-related antibodies and anti-inflammatory ability	1439:1502	Combined with our previous results, the efficacy of IVIG against AD may be positively correlated with its level of AD-related antibodies and anti-inflammatory ability.
36982622	5	45	theme	IVIG	691:694	arg1	composition					663:673	composition	663:673	composition	663:673	In order to investigate the relationship between the composition and function of IVIG and its efficacy in treating AD, we selected three IVIGs that showed notable differences in therapeutic effects.
36982622	5	45	theme	IVIG	691:694	arg1	function					679:686	function	679:686	function	679:686	In order to investigate the relationship between the composition and function of IVIG and its efficacy in treating AD, we selected three IVIGs that showed notable differences in therapeutic effects.
36982622	1	46	contain	has	226:228	arg1	disease					160:166	Alzheimer's disease	148:166	Alzheimer's disease (AD)	148:171	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	1	46	contain	has	226:228	arg1	disease					203:209	a common neurodegenerative disease	176:209	a common neurodegenerative disease that currently has no known cure	176:242	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	1	46	contain	has	226:228	arg2	cure					239:242	no known cure	230:242	no known cure	230:242	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	8	47	theme	IVIG	1389:1392	arg1	efficacy					1377:1384	the efficacy	1373:1384	the efficacy of IVIG against AD	1373:1403	Combined with our previous results, the efficacy of IVIG against AD may be positively correlated with its level of AD-related antibodies and anti-inflammatory ability.
36982622	7	48	from	injury	1272:1277	arg1	mice					1312:1315	Balb/c mice	1305:1315	Balb/c mice	1305:1315	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	7	49	theme	liver	1255:1259	arg1	injury					1272:1277	liver and kidney injury	1255:1277	liver and kidney injury	1255:1277	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	2	50	contain	has	321:323	arg1	immunoglobulin					257:270	Intravenous immunoglobulin	245:270	Intravenous immunoglobulin (IVIG)	245:277	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	2	50	contain	has	321:323	arg2	properties					343:352	anti-inflammatory properties	325:352	anti-inflammatory properties	325:352	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	2	50	contain	has	321:323	arg1	IVIG					273:276	IVIG	273:276	IVIG	273:276	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	6	51	theme	specific	837:844	arg1	antibodies					846:855	specific antibodies	837:855	specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau)	837:922	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	7	52	theme	varying	1320:1326	arg1	degrees					1328:1334	varying degrees	1320:1334	varying degrees	1320:1334	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	7	53	theme	peripheral	1230:1239	arg1	inflammation					1241:1252	LPS-stimulated peripheral inflammation	1215:1252	LPS-stimulated peripheral inflammation	1215:1252	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	9	54	theme	IVIG	1563:1566	arg1	analysis					1525:1532	AD-related antibody analysis	1505:1532	AD-related antibody analysis	1505:1532	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	9	54	theme	IVIG	1563:1566	arg1	evaluation					1549:1558	functional evaluation	1538:1558	functional evaluation	1538:1558	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	9	55	theme	clinical	1612:1619	arg1	trials					1621:1626	clinical trials	1612:1626	clinical trials	1612:1626	AD-related antibody analysis and functional evaluation of IVIG should be given sufficient attention before clinical trials, as this may greatly affect the therapeutic effect of AD treatment.
36982622	2	56	theme	anti-inflammatory	325:341	arg1	properties					343:352	anti-inflammatory properties	325:352	anti-inflammatory properties	325:352	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	1	57	theme	known	233:237	arg1	cure					239:242	no known cure	230:242	no known cure	230:242	Alzheimer's disease (AD) is a common neurodegenerative disease that currently has no known cure.
36982622	7	58	theme	anti-p-tau	1184:1193	arg1	ratio					1195:1199	anti-p-tau ratio	1184:1199	anti-p-tau ratio	1184:1199	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	7	59	theme	kidney	1265:1270	arg1	injury					1272:1277	liver and kidney injury	1255:1277	liver and kidney injury	1255:1277	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	7	60	from	neuroinflammation	1284:1300	arg1	mice					1312:1315	Balb/c mice	1305:1315	Balb/c mice	1305:1315	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	7	61	theme	Balb/c	1305:1310	arg1	mice					1312:1315	Balb/c mice	1305:1315	Balb/c mice	1305:1315	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	6	62	theme	systemic	968:975	arg1	inflammation					977:988	systemic inflammation	968:988	systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice	968:1039	Then, the concentrations of specific antibodies against β-amyloid (Aβ)42, tau, and hyperphosphorylated tau (p-tau) in three IVIGs, as well as their effects on systemic inflammation induced by lipopolysaccharide (LPS) in Balb/c mice, were analyzed and compared in this study.
36982622	0	63	theme	Potential	72:80	arg1	Evaluation					46:55	Anti-Inflammatory Function Evaluation	19:55	Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease	19:145	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	0	63	theme	Potential	72:80	arg1	Assay					9:13	Antibody Assay	0:13	Antibody Assay	0:13	Antibody Assay and Anti-Inflammatory Function Evaluation of Therapeutic Potential of Different Intravenous Immunoglobulins for Alzheimer's Disease.
36982622	8	64	theme	AD-related	1452:1461	arg1	antibodies					1463:1472	AD-related antibodies	1452:1472	AD-related antibodies	1452:1472	Combined with our previous results, the efficacy of IVIG against AD may be positively correlated with its level of AD-related antibodies and anti-inflammatory ability.
36982622	2	65	theme	Intravenous	245:255	arg1	immunoglobulin					257:270	Intravenous immunoglobulin	245:270	Intravenous immunoglobulin (IVIG)	245:277	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	2	65	theme	Intravenous	245:255	arg1	IVIG					273:276	IVIG	273:276	IVIG	273:276	Intravenous immunoglobulin (IVIG), which contains AD-related antibodies and has anti-inflammatory properties, has shown potential as a treatment for AD.
36982622	7	66	theme	LPS-stimulated	1215:1228	arg1	inflammation					1241:1252	LPS-stimulated peripheral inflammation	1215:1252	LPS-stimulated peripheral inflammation	1215:1252	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
36982622	7	67	theme	antibody	1157:1164	arg1	concentration					1166:1178	anti-Aβ42/tau antibody concentration	1143:1178	anti-Aβ42/tau antibody concentration	1143:1178	The results indicated that these IVIGs differed greatly in anti-Aβ42/tau antibody concentration and anti-p-tau ratio, and improved LPS-stimulated peripheral inflammation, liver and kidney injury, and neuroinflammation in Balb/c mice to varying degrees.
35834299	12	0	theme	=LMG	1516:1519	arg1	JJ-63T					1508:1513	JJ-63T	1508:1513	JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain	1508:1581	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	12	0	theme	=LMG	1516:1519	arg1	111899T					1555:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	7	1	theme	polar	883:887	arg1	profile					895:901	The polar lipid profile	879:901	The polar lipid profile	879:901	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	12	2	theme	type	1571:1574	arg1	strain					1576:1581	the type strain	1567:1581	the type strain	1567:1581	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	4	3	theme	16S	494:496	arg1	gene					503:506	the 16S rRNA gene	490:506	the 16S rRNA gene	490:506	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes placed the strain into the Cereus clade.
35834299	3	4	theme	strain	285:290	arg1	JJ-63T					292:297	strain JJ-63T	285:297	strain JJ-63T	285:297	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	3	4	theme	strain	285:290	arg1	member					304:309	a member	302:309	a member of the genus Bacillus	302:331	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	6	5	theme	quinone	804:810	arg1	system					812:817	The quinone system	800:817	The quinone system of strain JJ-63T	800:834	The quinone system of strain JJ-63T consisted exclusively of menaquinone MK-7.
35834299	4	6	theme	phylogenetic	459:470	arg1	analysis					472:479	Detailed phylogenetic analysis	450:479	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes	450:565	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes placed the strain into the Cereus clade.
35834299	4	7	theme	Cereus	594:599	arg1	clade					601:605	the Cereus clade	590:605	the Cereus clade	590:605	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes placed the strain into the Cereus clade.
35834299	10	8	theme	related	1350:1356	arg1	species					1358:1364	the most closely related species	1333:1364	the most closely related species	1333:1364	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	5	9	theme	identity	631:638	arg1	values					703:708	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	608:708	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides	608:753	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	8	10	dep	compounds	1104:1112	arg1	 0					1123:1124	 0	1123:1124	 0	1123:1124	Major fatty acids were iso- and anteiso-branched with the major compounds iso-C15 : 0 and iso-C17 : 0.
35834299	8	10	dep	compounds	1104:1112	arg1	 0					1139:1140	 0	1139:1140	 0	1139:1140	Major fatty acids were iso- and anteiso-branched with the major compounds iso-C15 : 0 and iso-C17 : 0.
35834299	10	11	theme	JJ-63T	1321:1326	arg1	differentiation					1295:1309	a further phenotypic differentiation	1274:1309	a further phenotypic differentiation of strain JJ-63T from the most closely related species	1274:1364	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	8	12	theme	major	1098:1102	arg1	compounds					1104:1112	the major compounds iso-C15 : 0 and iso-C17 : 0	1094:1140	the major compounds iso-C15 : 0 and iso-C17 : 0	1094:1140	Major fatty acids were iso- and anteiso-branched with the major compounds iso-C15 : 0 and iso-C17 : 0.
35834299	10	13	theme	strain	1314:1319	arg1	JJ-63T					1321:1326	strain JJ-63T	1314:1326	strain JJ-63T	1314:1326	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	4	14	theme	rRNA	498:501	arg1	gene					503:506	the 16S rRNA gene	490:506	the 16S rRNA gene	490:506	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes placed the strain into the Cereus clade.
35834299	5	15	theme	average	641:647	arg1	identity					660:667	average amino acid identity	641:667	average amino acid identity	641:667	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	4	16	theme	phylum	549:554	arg1	Firmicutes					556:565	the phylum Firmicutes	545:565	the phylum Firmicutes	545:565	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes placed the strain into the Cereus clade.
35834299	12	17	theme	9090T=DSM	1532:1540	arg1	JJ-63T					1508:1513	JJ-63T	1508:1513	JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain	1508:1581	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	12	17	theme	9090T=DSM	1532:1540	arg1	111899T					1555:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	1	18	from	roots	41:45	arg1	nov.					25:28	nov.	25:28	nov. from maize roots.	25:46	nov. from maize roots.
35834299	7	19	dep	components	926:935	arg1	phosphatidylglycerol					987:1006	phosphatidylglycerol	987:1006	phosphatidylglycerol	987:1006	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	19	dep	components	926:935	arg1	components					926:935	the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid	916:1037	the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid	916:1037	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	19	dep	components	926:935	arg1	phosphatidylethanolamine					961:984	phosphatidylethanolamine	961:984	phosphatidylethanolamine	961:984	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	19	dep	components	926:935	arg1	glycolipid					1028:1037	an unidentified glycolipid	1012:1037	an unidentified glycolipid	1012:1037	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	19	dep	components	926:935	arg1	diphosphatidylglycerol					937:958	diphosphatidylglycerol	937:958	diphosphatidylglycerol	937:958	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	5	20	theme	amino	649:653	arg1	identity					660:667	average amino acid identity	641:667	average amino acid identity	641:667	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	2	21	theme	root	137:140	arg1	surface					142:148	the root surface	133:148	the root surface of maize (Zea mays)	133:168	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	3	22	dep	related	347:353	arg1	followed					411:418	followed	411:418	followed by Bacillus cereus (98.47 %)	411:447	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	3	22	dep	related	347:353	arg1	member					304:309	a member	302:309	a member of the genus Bacillus	302:331	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	3	22	dep	related	347:353	arg1	JJ-63T					292:297	strain JJ-63T	285:297	strain JJ-63T	285:297	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	9	23	theme	characteristic	1153:1166	arg1	compounds					1168:1176	the characteristic compounds	1149:1176	the characteristic compounds C13 : 0 iso and C16 : 1 cis10	1149:1206	Also, the characteristic compounds C13 : 0 iso and C16 : 1 cis10 were found.
35834299	5	24	theme	type	722:725	arg1	strain					727:732	the type strain	718:732	the type strain of B. pseudomycoides	718:753	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	8	25	theme	Major	1040:1044	arg1	acids					1052:1056	Major fatty acids	1040:1056	Major fatty acids	1040:1056	Major fatty acids were iso- and anteiso-branched with the major compounds iso-C15 : 0 and iso-C17 : 0.
35834299	6	26	theme	JJ-63T	829:834	arg1	system					812:817	The quinone system	800:817	The quinone system of strain JJ-63T	800:834	The quinone system of strain JJ-63T consisted exclusively of menaquinone MK-7.
35834299	0	27	theme	rhizoplanae	9:19	arg1	sp					21:22	Bacillus rhizoplanae sp	0:22	Bacillus rhizoplanae sp.	0:23	Bacillus rhizoplanae sp.
35834299	5	28	theme	digital	673:679	arg1	hybridization					689:701	digital DNA-DNA hybridization	673:701	digital DNA-DNA hybridization	673:701	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	7	29	theme	unidentified	1015:1026	arg1	components					926:935	the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid	916:1037	the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid	916:1037	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	29	theme	unidentified	1015:1026	arg1	glycolipid					1028:1037	an unidentified glycolipid	1012:1037	an unidentified glycolipid	1012:1037	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	3	30	theme	type	362:365	arg1	strain					367:372	the type strain	358:372	the type strain of Bacillus pseudomycoides (98.61%)	358:408	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	0	31	theme	Bacillus	0:7	arg1	sp					21:22	Bacillus rhizoplanae sp	0:22	Bacillus rhizoplanae sp.	0:23	Bacillus rhizoplanae sp.
35834299	6	32	theme	strain	822:827	arg1	JJ-63T					829:834	strain JJ-63T	822:834	strain JJ-63T	822:834	The quinone system of strain JJ-63T consisted exclusively of menaquinone MK-7.
35834299	11	33	theme	Bacillus	1460:1467	arg1	sp					1481:1482	Bacillus rhizoplanae sp	1460:1482	the name Bacillus rhizoplanae sp	1451:1482	For this reason, JJ-63T represents a novel species of the genus Bacillus, for which the name Bacillus rhizoplanae sp.
35834299	10	34	theme	Physiological	1220:1232	arg1	characteristics					1250:1264	Physiological and biochemical characteristics	1220:1264	Physiological and biochemical characteristics	1220:1264	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	5	35	theme	26.30 	777:782	arg1	%					783:783	80.97, 81.45 and 26.30 %	760:783	%	783:783	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	2	36	theme	Gram-stain-positive	50:68	arg1	strain					111:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	3	37	theme	16S	238:240	arg1	comparisons					272:282	16S rRNA gene sequence similarity comparisons	238:282	16S rRNA gene sequence similarity comparisons	238:282	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	11	38	theme	rhizoplanae	1469:1479	arg1	sp					1481:1482	Bacillus rhizoplanae sp	1460:1482	the name Bacillus rhizoplanae sp	1451:1482	For this reason, JJ-63T represents a novel species of the genus Bacillus, for which the name Bacillus rhizoplanae sp.
35834299	12	39	theme	32091T=CCM	1521:1530	arg1	JJ-63T					1508:1513	JJ-63T	1508:1513	JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain	1508:1581	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	12	39	theme	32091T=CCM	1521:1530	arg1	111899T					1555:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	5	40	theme	B.	737:738	arg1	pseudomycoides					740:753	B. pseudomycoides	737:753	B. pseudomycoides	737:753	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	3	41	theme	Bacillus	324:331	arg1	JJ-63T					292:297	strain JJ-63T	285:297	strain JJ-63T	285:297	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	3	41	theme	Bacillus	324:331	arg1	member					304:309	a member	302:309	a member of the genus Bacillus	302:331	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	6	42	theme	menaquinone	861:871	arg1	MK-7					873:876	menaquinone MK-7	861:876	menaquinone MK-7	861:876	The quinone system of strain JJ-63T consisted exclusively of menaquinone MK-7.
35834299	3	43	theme	rRNA	242:245	arg1	comparisons					272:282	16S rRNA gene sequence similarity comparisons	238:282	16S rRNA gene sequence similarity comparisons	238:282	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	12	44	theme	111827T=	1542:1549	arg1	JJ-63T					1508:1513	JJ-63T	1508:1513	JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain	1508:1581	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	12	44	theme	111827T=	1542:1549	arg1	111899T					1555:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	12	45	theme	CIP	1551:1553	arg1	JJ-63T					1508:1513	JJ-63T	1508:1513	JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain	1508:1581	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	12	45	theme	CIP	1551:1553	arg1	111899T					1555:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T	1516:1561	nov. is proposed, with JJ-63T (=LMG 32091T=CCM 9090T=DSM 111827T= CIP 111899T) as the type strain.
35834299	5	46	theme	pseudomycoides	740:753	arg1	strain					727:732	the type strain	718:732	the type strain of B. pseudomycoides	718:753	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	5	47	theme	DNA-DNA	681:687	arg1	hybridization					689:701	digital DNA-DNA hybridization	673:701	digital DNA-DNA hybridization	673:701	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	5	48	theme	average	612:618	arg1	identity					631:638	The average nucleotide identity	608:638	The average nucleotide identity	608:638	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	2	49	theme	Zea	160:162	arg1	maize					153:157	maize	153:157	maize (Zea mays)	153:168	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	2	49	theme	Zea	160:162	arg1	mays					164:167	Zea mays	160:167	Zea mays	160:167	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	11	50	dep	name	1455:1458	arg1	sp					1481:1482	Bacillus rhizoplanae sp	1460:1482	the name Bacillus rhizoplanae sp	1451:1482	For this reason, JJ-63T represents a novel species of the genus Bacillus, for which the name Bacillus rhizoplanae sp.
35834299	3	51	theme	pseudomycoides	386:399	arg1	strain					367:372	the type strain	358:372	the type strain of Bacillus pseudomycoides (98.61%)	358:408	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	5	52	theme	hybridization	689:701	arg1	values					703:708	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	608:708	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides	608:753	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	2	53	theme	bacterial	101:109	arg1	strain					111:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	5	54	theme	nucleotide	620:629	arg1	identity					631:638	The average nucleotide identity	608:638	The average nucleotide identity	608:638	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	5	55	theme	80.97	760:764	arg1	%					783:783	80.97, 81.45 and 26.30 %	760:783	%	783:783	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	2	56	theme	endospore-forming	83:99	arg1	strain					111:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	3	57	theme	similarity	261:270	arg1	comparisons					272:282	16S rRNA gene sequence similarity comparisons	238:282	16S rRNA gene sequence similarity comparisons	238:282	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	2	58	theme	maize	153:157	arg1	surface					142:148	the root surface	133:148	the root surface of maize (Zea mays)	133:168	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	7	59	theme	major	920:924	arg1	phosphatidylglycerol					987:1006	phosphatidylglycerol	987:1006	phosphatidylglycerol	987:1006	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	59	theme	major	920:924	arg1	components					926:935	the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid	916:1037	the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid	916:1037	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	59	theme	major	920:924	arg1	phosphatidylethanolamine					961:984	phosphatidylethanolamine	961:984	phosphatidylethanolamine	961:984	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	59	theme	major	920:924	arg1	glycolipid					1028:1037	an unidentified glycolipid	1012:1037	an unidentified glycolipid	1012:1037	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	7	59	theme	major	920:924	arg1	diphosphatidylglycerol					937:958	diphosphatidylglycerol	937:958	diphosphatidylglycerol	937:958	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	4	60	theme	Detailed	450:457	arg1	analysis					472:479	Detailed phylogenetic analysis	450:479	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes	450:565	Detailed phylogenetic analysis based on the 16S rRNA gene and the 87 proteins conserved within the phylum Firmicutes placed the strain into the Cereus clade.
35834299	8	61	theme	fatty	1046:1050	arg1	acids					1052:1056	Major fatty acids	1040:1056	Major fatty acids	1040:1056	Major fatty acids were iso- and anteiso-branched with the major compounds iso-C15 : 0 and iso-C17 : 0.
35834299	10	62	theme	biochemical	1238:1248	arg1	characteristics					1250:1264	Physiological and biochemical characteristics	1220:1264	Physiological and biochemical characteristics	1220:1264	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	2	63	theme	aerobic	71:77	arg1	strain					111:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	3	64	theme	gene	247:250	arg1	comparisons					272:282	16S rRNA gene sequence similarity comparisons	238:282	16S rRNA gene sequence similarity comparisons	238:282	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	9	65	theme	 0	1183:1184	arg1	iso					1186:1188	 0 iso	1183:1188	 0 iso	1183:1188	Also, the characteristic compounds C13 : 0 iso and C16 : 1 cis10 were found.
35834299	10	66	from	species	1358:1364	arg1	differentiation					1295:1309	a further phenotypic differentiation	1274:1309	a further phenotypic differentiation of strain JJ-63T from the most closely related species	1274:1364	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	9	67	dep	compounds	1168:1176	arg1	C13 					1178:1181	C13 	1178:1181	the characteristic compounds C13 : 0 iso and C16 : 1 cis10	1149:1206	Also, the characteristic compounds C13 : 0 iso and C16 : 1 cis10 were found.
35834299	3	68	theme	sequence	252:259	arg1	comparisons					272:282	16S rRNA gene sequence similarity comparisons	238:282	16S rRNA gene sequence similarity comparisons	238:282	It could be clearly shown that, based on 16S rRNA gene sequence similarity comparisons, strain JJ-63T is a member of the genus Bacillus, most closely related to the type strain of Bacillus pseudomycoides (98.61%), followed by Bacillus cereus (98.47 %).
35834299	7	69	theme	lipid	889:893	arg1	profile					895:901	The polar lipid profile	879:901	The polar lipid profile	879:901	The polar lipid profile consisted of the major components diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
35834299	11	70	theme	Bacillus	1431:1438	arg1	species					1410:1416	a novel species	1402:1416	a novel species	1402:1416	For this reason, JJ-63T represents a novel species of the genus Bacillus, for which the name Bacillus rhizoplanae sp.
35834299	9	71	dep	C13 	1178:1181	arg1	C16 					1194:1197	C16 	1194:1197	C16 	1194:1197	Also, the characteristic compounds C13 : 0 iso and C16 : 1 cis10 were found.
35834299	9	71	dep	C13 	1178:1181	arg1	cis10					1202:1206	 1 cis10	1199:1206	the characteristic compounds C13 : 0 iso and C16 : 1 cis10	1149:1206	Also, the characteristic compounds C13 : 0 iso and C16 : 1 cis10 were found.
35834299	9	71	dep	C13 	1178:1181	arg1	iso					1186:1188	 0 iso	1183:1188	 0 iso	1183:1188	Also, the characteristic compounds C13 : 0 iso and C16 : 1 cis10 were found.
35834299	5	72	theme	acid	655:658	arg1	identity					660:667	average amino acid identity	641:667	average amino acid identity	641:667	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	1	73	theme	maize	35:39	arg1	roots					41:45	maize roots	35:45	maize roots	35:45	nov. from maize roots.
35834299	10	74	theme	phenotypic	1284:1293	arg1	differentiation					1295:1309	a further phenotypic differentiation	1274:1309	a further phenotypic differentiation of strain JJ-63T from the most closely related species	1274:1364	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	2	75	attach	isolated	119:126	arg2	strain					111:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain	48:116	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	2	75	attach	isolated	119:126	arg1	surface					142:148	the root surface	133:148	the root surface of maize (Zea mays)	133:168	A Gram-stain-positive, aerobic and endospore-forming bacterial strain, isolated from the root surface of maize (Zea mays) was taxonomically studied.
35834299	11	76	theme	novel	1404:1408	arg1	species					1410:1416	a novel species	1402:1416	a novel species	1402:1416	For this reason, JJ-63T represents a novel species of the genus Bacillus, for which the name Bacillus rhizoplanae sp.
35834299	5	77	theme	identity	660:667	arg1	values					703:708	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	608:708	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides	608:753	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values against the type strain of B. pseudomycoides were 80.97, 81.45 and 26.30 %, respectively.
35834299	9	78	theme	 1	1199:1200	arg1	cis10					1202:1206	 1 cis10	1199:1206	the characteristic compounds C13 : 0 iso and C16 : 1 cis10	1149:1206	Also, the characteristic compounds C13 : 0 iso and C16 : 1 cis10 were found.
35834299	10	79	theme	further	1276:1282	arg1	differentiation					1295:1309	a further phenotypic differentiation	1274:1309	a further phenotypic differentiation of strain JJ-63T from the most closely related species	1274:1364	Physiological and biochemical characteristics allowed a further phenotypic differentiation of strain JJ-63T from the most closely related species.
35834299	8	80	with	iso-	1063:1066	arg1	compounds					1104:1112	the major compounds iso-C15 : 0 and iso-C17 : 0	1094:1140	the major compounds iso-C15 : 0 and iso-C17 : 0	1094:1140	Major fatty acids were iso- and anteiso-branched with the major compounds iso-C15 : 0 and iso-C17 : 0.
37268931	8	0	theme	rDNA	1160:1163	arg1	sequencing					1165:1174	16 s rDNA sequencing	1155:1174	16 s rDNA sequencing	1155:1174	Western blotting was performed to evaluate protein expression, whereas alterations in gut microbiota were determined by 16 s rDNA sequencing.
37268931	12	1	theme	villi	1847:1851	arg1	structures					1853:1862	the intestinal mucosal and villi structures	1820:1862	the intestinal mucosal and villi structures	1820:1862	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	8	2	dep	performed	1056:1064	arg1	whereas					1098:1104	whereas	1098:1104	whereas	1098:1104	Western blotting was performed to evaluate protein expression, whereas alterations in gut microbiota were determined by 16 s rDNA sequencing.
37268931	6	3	theme	inflammatory	790:801	arg1	biomarkers					803:812	inflammatory biomarkers	790:812	inflammatory biomarkers	790:812	The levels of inflammatory biomarkers and myocardial damage biomarkers were measured through ELISA, while echocardiography was used to assess heart function.
37268931	2	4	theme	anti-inflammatory	344:360	arg1	properties					362:371	anti-inflammatory properties	344:371	anti-inflammatory properties that found to be effective against CAD	344:410	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	1	5	theme	gut	250:252	arg1	microbiota					254:263	the gut microbiota	246:263	the gut microbiota	246:263	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	10	6	theme	TLR4	1402:1405	arg1	expression					1377:1386	the protein expression	1365:1386	the protein expression of myocardial TLR4, MyD88, and p-P65	1365:1423	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	2	7	theme	SMYA	283:286	arg1	formula					331:337	a traditional Chinese herbal formula	302:337	a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD	302:410	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	2	7	theme	SMYA	283:286	arg1	decoction					289:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	9	8	theme	cardiac	1211:1217	arg1	function					1219:1226	cardiac function	1211:1226	cardiac function	1211:1226	RESULTS SMYA was found to enhance cardiac function and decrease the expression of serum CK-MB and LDH.
37268931	2	9	theme	Si-Miao-Yong-An	266:280	arg1	formula					331:337	a traditional Chinese herbal formula	302:337	a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD	302:410	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	2	9	theme	Si-Miao-Yong-An	266:280	arg1	decoction					289:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	14	10	theme	TLR4/NF-κB	2220:2229	arg1	pathway					2241:2247	the LPS-induced TLR4/NF-κB signaling pathway	2204:2247	the LPS-induced TLR4/NF-κB signaling pathway	2204:2247	SMYA was also found to inhibit the LPS-induced TLR4/NF-κB signaling pathway, leading to a decrease in the release of inflammatory factors, which ultimately mitigated myocardial injury.
37268931	15	11	theme	therapeutic	2389:2399	arg1	agent					2401:2405	a therapeutic agent	2387:2405	a therapeutic agent for the management of CAD	2387:2431	Hence, SMYA holds promise as a therapeutic agent for the management of CAD.
37268931	15	11	theme	therapeutic	2389:2399	arg1	promise					2376:2382	promise	2376:2382	promise	2376:2382	Hence, SMYA holds promise as a therapeutic agent for the management of CAD.
37268931	1	12	attach	linked	236:241	arg2	inflammation					206:217	inflammation	206:217	inflammation which is closely linked to the gut microbiota	206:263	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	1	12	attach	linked	236:241	arg1	microbiota					254:263	the gut microbiota	246:263	the gut microbiota	246:263	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	10	13	theme	MyD88	1408:1412	arg1	expression					1377:1386	the protein expression	1365:1386	the protein expression of myocardial TLR4, MyD88, and p-P65	1365:1423	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	0	14	theme	signaling	123:131	arg1	pathway					133:139	LPS-induced TLR4/NF-κB signaling pathway	100:139	LPS-induced TLR4/NF-κB signaling pathway	100:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	14	15	theme	factors	2303:2309	arg1	release					2279:2285	the release	2275:2285	the release of inflammatory factors	2275:2309	SMYA was also found to inhibit the LPS-induced TLR4/NF-κB signaling pathway, leading to a decrease in the release of inflammatory factors, which ultimately mitigated myocardial injury.
37268931	4	16	theme	SMYA	644:647	arg1	extract					649:655	the SMYA extract	640:655	the SMYA extract	640:655	METHODS The identification of components in the SMYA extract was conducted using the HPLC method.
37268931	7	17	theme	H&E	1021:1023	arg1	staining					1025:1032	H&E staining	1021:1032	H&E staining	1021:1032	Histological alterations in the myocardial and colonic tissues were examined following H&E staining.
37268931	9	18	theme	CK-MB	1265:1269	arg1	expression					1245:1254	the expression	1241:1254	the expression of serum CK-MB and LDH	1241:1277	RESULTS SMYA was found to enhance cardiac function and decrease the expression of serum CK-MB and LDH.
37268931	3	19	theme	gut	580:582	arg1	microbiota					584:593	the gut microbiota	576:593	the gut microbiota	576:593	However, it is still unclear whether SMYA can modulate gut microbiota and whether it contributes to the improvement of CAD by reducing inflammation and regulating the gut microbiota.
37268931	6	20	theme	heart	918:922	arg1	function					924:931	heart function	918:931	heart function	918:931	The levels of inflammatory biomarkers and myocardial damage biomarkers were measured through ELISA, while echocardiography was used to assess heart function.
37268931	9	21	theme	LDH	1275:1277	arg1	expression					1245:1254	the expression	1241:1254	the expression of serum CK-MB and LDH	1241:1277	RESULTS SMYA was found to enhance cardiac function and decrease the expression of serum CK-MB and LDH.
37268931	1	22	theme	BACKGROUND	142:151	arg1	CAD					178:180	CAD	178:180	CAD	178:180	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	1	22	theme	BACKGROUND	142:151	arg1	Disease					169:175	BACKGROUND Coronary Artery Disease	142:175	BACKGROUND Coronary Artery Disease (CAD)	142:181	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	7	23	from	alterations	947:957	arg1	tissues					989:995	the myocardial and colonic tissues	962:995	the myocardial and colonic tissues	962:995	Histological alterations in the myocardial and colonic tissues were examined following H&E staining.
37268931	4	24	from	identification	608:621	arg1	extract					649:655	the SMYA extract	640:655	the SMYA extract	640:655	METHODS The identification of components in the SMYA extract was conducted using the HPLC method.
37268931	1	25	theme	Artery	162:167	arg1	CAD					178:180	CAD	178:180	CAD	178:180	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	1	25	theme	Artery	162:167	arg1	Disease					169:175	BACKGROUND Coronary Artery Disease	142:175	BACKGROUND Coronary Artery Disease (CAD)	142:181	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	11	26	theme	microbiota	1521:1530	arg1	composition					1502:1512	the composition	1498:1512	the composition of gut microbiota	1498:1530	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	0	27	theme	gut	74:76	arg1	microbiota					78:87	gut microbiota	74:87	gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway	74:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	12	28	theme	intestinal	1943:1952	arg1	permeability					1954:1965	intestinal permeability	1943:1965	intestinal permeability	1943:1965	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	0	29	from	Effects	0:6	arg1	rats					55:58	myocardial I/R rats	40:58	myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway	40:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	14	30	theme	myocardial	2339:2348	arg1	injury					2350:2355	myocardial injury	2339:2355	myocardial injury	2339:2355	SMYA was also found to inhibit the LPS-induced TLR4/NF-κB signaling pathway, leading to a decrease in the release of inflammatory factors, which ultimately mitigated myocardial injury.
37268931	6	31	used	used	903:906	arg2	echocardiography					882:897	echocardiography	882:897	echocardiography	882:897	The levels of inflammatory biomarkers and myocardial damage biomarkers were measured through ELISA, while echocardiography was used to assess heart function.
37268931	0	32	theme	LPS-induced	100:110	arg1	pathway					133:139	LPS-induced TLR4/NF-κB signaling pathway	100:139	LPS-induced TLR4/NF-κB signaling pathway	100:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	13	33	theme	intestinal	2093:2102	arg1	barrier					2104:2110	the intestinal barrier	2089:2110	the intestinal barrier	2089:2110	CONCLUSIONS The results indicate that SMYA has the potential to modulate the gut microbiota and protect the intestinal barrier, thereby reducing the translocation of LPS into circulation.
37268931	10	34	theme	serum	1452:1456	arg1	factors					1475:1481	serum pro-inflammatory factors	1452:1481	serum pro-inflammatory factors	1452:1481	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	14	35	theme	inflammatory	2290:2301	arg1	factors					2303:2309	inflammatory factors	2290:2309	inflammatory factors	2290:2309	SMYA was also found to inhibit the LPS-induced TLR4/NF-κB signaling pathway, leading to a decrease in the release of inflammatory factors, which ultimately mitigated myocardial injury.
37268931	0	36	theme	Si-Miao-Yong-An	11:25	arg1	decoction					27:35	Si-Miao-Yong-An decoction	11:35	Si-Miao-Yong-An decoction	11:35	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	4	37	theme	HPLC	681:684	arg1	method					686:691	the HPLC method	677:691	the HPLC method	677:691	METHODS The identification of components in the SMYA extract was conducted using the HPLC method.
37268931	11	38	theme	other	1757:1761	arg1	species					1773:1779	other bacterial species	1757:1779	other bacterial species	1757:1779	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	13	39	theme	gut	2062:2064	arg1	microbiota					2066:2075	the gut microbiota	2058:2075	the gut microbiota	2058:2075	CONCLUSIONS The results indicate that SMYA has the potential to modulate the gut microbiota and protect the intestinal barrier, thereby reducing the translocation of LPS into circulation.
37268931	0	40	theme	I/R	51:53	arg1	rats					55:58	myocardial I/R rats	40:58	myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway	40:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	6	41	theme	damage	829:834	arg1	biomarkers					836:845	myocardial damage biomarkers	818:845	myocardial damage biomarkers	818:845	The levels of inflammatory biomarkers and myocardial damage biomarkers were measured through ELISA, while echocardiography was used to assess heart function.
37268931	10	42	theme	signaling	1329:1337	arg1	pathway					1339:1345	the TLR4/NF-κB signaling pathway	1314:1345	the TLR4/NF-κB signaling pathway	1314:1345	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	2	43	theme	herbal	324:329	arg1	formula					331:337	a traditional Chinese herbal formula	302:337	a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD	302:410	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	2	43	theme	herbal	324:329	arg1	decoction					289:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	12	44	theme	junction	1897:1904	arg1	protein					1906:1912	tight junction protein	1891:1912	tight junction protein (ZO-1, occludin)	1891:1929	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	12	45	dep	protein	1906:1912	arg1	ZO-1					1915:1918	ZO-1	1915:1918	ZO-1	1915:1918	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	12	45	dep	protein	1906:1912	arg1	occludin					1921:1928	occludin	1921:1928	occludin	1921:1928	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	11	46	theme	LPS/TLR4/NF-κB	1653:1666	arg1	pathway					1668:1674	the LPS/TLR4/NF-κB pathway	1649:1674	the LPS/TLR4/NF-κB pathway	1649:1674	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	12	47	theme	tight	1891:1895	arg1	protein					1906:1912	tight junction protein	1891:1912	tight junction protein (ZO-1, occludin)	1891:1929	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	8	48	theme	16 s	1155:1158	arg1	sequencing					1165:1174	16 s rDNA sequencing	1155:1174	16 s rDNA sequencing	1155:1174	Western blotting was performed to evaluate protein expression, whereas alterations in gut microbiota were determined by 16 s rDNA sequencing.
37268931	2	49	with	formula	331:337	arg1	properties					362:371	anti-inflammatory properties	344:371	anti-inflammatory properties that found to be effective against CAD	344:410	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	11	50	attach	linked	1639:1644	arg2	Prevotellaceae_Ga6A1					1593:1612	Prevotellaceae_Ga6A1	1593:1612	Prevotellaceae_Ga6A1	1593:1612	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	11	50	attach	linked	1639:1644	arg1	pathway					1668:1674	the LPS/TLR4/NF-κB pathway	1649:1674	the LPS/TLR4/NF-κB pathway	1649:1674	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	11	51	theme	Firmicutes/Bacteroidetes	1550:1573	arg1	ratio					1575:1579	the Firmicutes/Bacteroidetes ratio	1546:1579	the Firmicutes/Bacteroidetes ratio	1546:1579	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	3	52	theme	CAD	532:534	arg1	improvement					517:527	the improvement	513:527	the improvement of CAD	513:534	However, it is still unclear whether SMYA can modulate gut microbiota and whether it contributes to the improvement of CAD by reducing inflammation and regulating the gut microbiota.
37268931	12	53	theme	intestinal	1824:1833	arg1	structures					1853:1862	the intestinal mucosal and villi structures	1820:1862	the intestinal mucosal and villi structures	1820:1862	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	10	54	theme	myocardial	1391:1400	arg1	TLR4					1402:1405	myocardial TLR4	1391:1405	myocardial TLR4	1391:1405	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	14	55	theme	signaling	2231:2239	arg1	pathway					2241:2247	the LPS-induced TLR4/NF-κB signaling pathway	2204:2247	the LPS-induced TLR4/NF-κB signaling pathway	2204:2247	SMYA was also found to inhibit the LPS-induced TLR4/NF-κB signaling pathway, leading to a decrease in the release of inflammatory factors, which ultimately mitigated myocardial injury.
37268931	10	56	theme	p-P65	1419:1423	arg1	expression					1377:1386	the protein expression	1365:1386	the protein expression of myocardial TLR4, MyD88, and p-P65	1365:1423	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	2	57	theme	traditional	304:314	arg1	formula					331:337	a traditional Chinese herbal formula	302:337	a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD	302:410	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	2	57	theme	traditional	304:314	arg1	decoction					289:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	4	58	from	extract	649:655	arg1	identification					608:621	The identification	604:621	METHODS The identification of components in the SMYA extract	596:655	METHODS The identification of components in the SMYA extract was conducted using the HPLC method.
37268931	14	59	theme	LPS-induced	2208:2218	arg1	pathway					2241:2247	the LPS-induced TLR4/NF-κB signaling pathway	2204:2247	the LPS-induced TLR4/NF-κB signaling pathway	2204:2247	SMYA was also found to inhibit the LPS-induced TLR4/NF-κB signaling pathway, leading to a decrease in the release of inflammatory factors, which ultimately mitigated myocardial injury.
37268931	10	60	from	reduction	1439:1447	arg1	factors					1475:1481	serum pro-inflammatory factors	1452:1481	serum pro-inflammatory factors	1452:1481	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	4	61	theme	components	626:635	arg1	identification					608:621	The identification	604:621	METHODS The identification of components in the SMYA extract	596:655	METHODS The identification of components in the SMYA extract was conducted using the HPLC method.
37268931	4	62	from	components	626:635	arg1	extract					649:655	the SMYA extract	640:655	the SMYA extract	640:655	METHODS The identification of components in the SMYA extract was conducted using the HPLC method.
37268931	9	63	theme	serum	1259:1263	arg1	CK-MB					1265:1269	serum CK-MB	1259:1269	serum CK-MB	1259:1269	RESULTS SMYA was found to enhance cardiac function and decrease the expression of serum CK-MB and LDH.
37268931	8	64	from	alterations	1106:1116	arg1	microbiota					1125:1134	gut microbiota	1121:1134	gut microbiota	1121:1134	Western blotting was performed to evaluate protein expression, whereas alterations in gut microbiota were determined by 16 s rDNA sequencing.
37268931	3	65	theme	gut	468:470	arg1	microbiota					472:481	gut microbiota	468:481	gut microbiota	468:481	However, it is still unclear whether SMYA can modulate gut microbiota and whether it contributes to the improvement of CAD by reducing inflammation and regulating the gut microbiota.
37268931	4	66	dep	METHODS	596:602	arg1	identification					608:621	The identification	604:621	METHODS The identification of components in the SMYA extract	596:655	METHODS The identification of components in the SMYA extract was conducted using the HPLC method.
37268931	1	67	theme	Coronary	153:160	arg1	CAD					178:180	CAD	178:180	CAD	178:180	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	1	67	theme	Coronary	153:160	arg1	Disease					169:175	BACKGROUND Coronary Artery Disease	142:175	BACKGROUND Coronary Artery Disease (CAD)	142:181	BACKGROUND Coronary Artery Disease (CAD) is primarily caused by inflammation which is closely linked to the gut microbiota.
37268931	11	68	theme	gut	1517:1519	arg1	microbiota					1521:1530	gut microbiota	1517:1530	gut microbiota	1517:1530	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	13	69	theme	LPS	2151:2153	arg1	translocation					2134:2146	the translocation	2130:2146	the translocation of LPS into circulation	2130:2170	CONCLUSIONS The results indicate that SMYA has the potential to modulate the gut microbiota and protect the intestinal barrier, thereby reducing the translocation of LPS into circulation.
37268931	8	70	theme	Western	1035:1041	arg1	blotting					1043:1050	Western blotting	1035:1050	Western blotting	1035:1050	Western blotting was performed to evaluate protein expression, whereas alterations in gut microbiota were determined by 16 s rDNA sequencing.
37268931	7	71	theme	colonic	981:987	arg1	tissues					989:995	the myocardial and colonic tissues	962:995	the myocardial and colonic tissues	962:995	Histological alterations in the myocardial and colonic tissues were examined following H&E staining.
37268931	5	72	theme	groups	710:715	arg1	total					696:700	A total	694:700	A total of four groups of SD rats	694:726	A total of four groups of SD rats were orally administered with SMYA for 28 days.
37268931	7	73	theme	myocardial	966:975	arg1	tissues					989:995	the myocardial and colonic tissues	962:995	the myocardial and colonic tissues	962:995	Histological alterations in the myocardial and colonic tissues were examined following H&E staining.
37268931	13	74	dep	CONCLUSIONS	1985:1995	arg1	indicate					2009:2016	indicate	2009:2016	indicate that SMYA has the potential to modulate the gut microbiota and protect the intestinal barrier, thereby reducing the translocation of LPS into circulation	2009:2170	CONCLUSIONS The results indicate that SMYA has the potential to modulate the gut microbiota and protect the intestinal barrier, thereby reducing the translocation of LPS into circulation.
37268931	0	75	theme	TLR4/NF-κB	112:121	arg1	pathway					133:139	LPS-induced TLR4/NF-κB signaling pathway	100:139	LPS-induced TLR4/NF-κB signaling pathway	100:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	14	76	from	decrease	2263:2270	arg1	release					2279:2285	the release	2275:2285	the release of inflammatory factors	2275:2309	SMYA was also found to inhibit the LPS-induced TLR4/NF-κB signaling pathway, leading to a decrease in the release of inflammatory factors, which ultimately mitigated myocardial injury.
37268931	10	77	theme	pro-inflammatory	1458:1473	arg1	factors					1475:1481	serum pro-inflammatory factors	1452:1481	serum pro-inflammatory factors	1452:1481	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	5	78	theme	SD	720:721	arg1	rats					723:726	SD rats	720:726	SD rats	720:726	A total of four groups of SD rats were orally administered with SMYA for 28 days.
37268931	8	79	theme	protein	1078:1084	arg1	expression					1086:1095	protein expression	1078:1095	protein expression	1078:1095	Western blotting was performed to evaluate protein expression, whereas alterations in gut microbiota were determined by 16 s rDNA sequencing.
37268931	7	80	theme	Histological	934:945	arg1	alterations					947:957	Histological alterations	934:957	Histological alterations in the myocardial and colonic tissues	934:995	Histological alterations in the myocardial and colonic tissues were examined following H&E staining.
37268931	0	81	dep	microbiota	78:87	arg1	inhibit					92:98	inhibit	92:98	to inhibit LPS-induced TLR4/NF-κB signaling pathway	89:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	13	82	contain	has	2028:2030	arg1	SMYA					2023:2026	SMYA	2023:2026	SMYA	2023:2026	CONCLUSIONS The results indicate that SMYA has the potential to modulate the gut microbiota and protect the intestinal barrier, thereby reducing the translocation of LPS into circulation.
37268931	13	82	contain	has	2028:2030	arg2	potential					2036:2044	the potential to modulate the gut microbiota and protect the intestinal barrier	2032:2110	the potential to modulate the gut microbiota and protect the intestinal barrier	2032:2110	CONCLUSIONS The results indicate that SMYA has the potential to modulate the gut microbiota and protect the intestinal barrier, thereby reducing the translocation of LPS into circulation.
37268931	10	83	theme	TLR4/NF-κB	1318:1327	arg1	pathway					1339:1345	the TLR4/NF-κB signaling pathway	1314:1345	the TLR4/NF-κB signaling pathway	1314:1345	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
37268931	0	84	theme	decoction	27:35	arg1	Effects					0:6	Effects	0:6	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.	0:140	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	0	85	theme	myocardial	40:49	arg1	rats					55:58	myocardial I/R rats	40:58	myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway	40:139	Effects of Si-Miao-Yong-An decoction on myocardial I/R rats by regulating gut microbiota to inhibit LPS-induced TLR4/NF-κB signaling pathway.
37268931	5	86	theme	rats	723:726	arg1	groups					710:715	four groups	705:715	four groups of SD rats	705:726	A total of four groups of SD rats were orally administered with SMYA for 28 days.
37268931	5	86	theme	rats	723:726	arg1	rats					723:726	SD rats	720:726	SD rats	720:726	A total of four groups of SD rats were orally administered with SMYA for 28 days.
37268931	8	87	theme	gut	1121:1123	arg1	microbiota					1125:1134	gut microbiota	1121:1134	gut microbiota	1121:1134	Western blotting was performed to evaluate protein expression, whereas alterations in gut microbiota were determined by 16 s rDNA sequencing.
37268931	6	88	theme	biomarkers	836:845	arg1	levels					780:785	The levels	776:785	The levels of inflammatory biomarkers and myocardial damage biomarkers	776:845	The levels of inflammatory biomarkers and myocardial damage biomarkers were measured through ELISA, while echocardiography was used to assess heart function.
37268931	15	89	theme	CAD	2429:2431	arg1	management					2415:2424	the management	2411:2424	the management of CAD	2411:2431	Hence, SMYA holds promise as a therapeutic agent for the management of CAD.
37268931	12	90	theme	protein	1906:1912	arg1	expression					1877:1886	the expression	1873:1886	the expression of tight junction protein (ZO-1, occludin)	1873:1929	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	6	91	theme	myocardial	818:827	arg1	biomarkers					836:845	myocardial damage biomarkers	818:845	myocardial damage biomarkers	818:845	The levels of inflammatory biomarkers and myocardial damage biomarkers were measured through ELISA, while echocardiography was used to assess heart function.
37268931	9	92	theme	RESULTS	1177:1183	arg1	SMYA					1185:1188	RESULTS SMYA	1177:1188	RESULTS SMYA	1177:1188	RESULTS SMYA was found to enhance cardiac function and decrease the expression of serum CK-MB and LDH.
37268931	11	93	theme	bacterial	1763:1771	arg1	species					1773:1779	other bacterial species	1757:1779	other bacterial species	1757:1779	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	2	94	theme	Chinese	316:322	arg1	formula					331:337	a traditional Chinese herbal formula	302:337	a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD	302:410	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	2	94	theme	Chinese	316:322	arg1	decoction					289:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction	266:297	Si-Miao-Yong-An (SMYA) decoction is a traditional Chinese herbal formula with anti-inflammatory properties that found to be effective against CAD.
37268931	6	95	theme	biomarkers	803:812	arg1	levels					780:785	The levels	776:785	The levels of inflammatory biomarkers and myocardial damage biomarkers	776:845	The levels of inflammatory biomarkers and myocardial damage biomarkers were measured through ELISA, while echocardiography was used to assess heart function.
37268931	12	96	theme	mucosal	1835:1841	arg1	structures					1853:1862	the intestinal mucosal and villi structures	1820:1862	the intestinal mucosal and villi structures	1820:1862	Moreover, SMYA was found to safeguard the intestinal mucosal and villi structures, elevate the expression of tight junction protein (ZO-1, occludin), and reduce intestinal permeability and inflammation.
37268931	11	97	theme	beneficial	1692:1701	arg1	microbiota					1703:1712	beneficial microbiota	1692:1712	beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species	1692:1779	SMYA modified the composition of gut microbiota by decreasing the Firmicutes/Bacteroidetes ratio, modulating Prevotellaceae_Ga6A1 and Prevotellaceae_NK3B3 linked to the LPS/TLR4/NF-κB pathway, and increasing beneficial microbiota such as Bacteroidetes, Alloprevotella, and other bacterial species.
37268931	10	98	theme	protein	1369:1375	arg1	expression					1377:1386	the protein expression	1365:1386	the protein expression of myocardial TLR4, MyD88, and p-P65	1365:1423	SMYA was also observed to inhibit the TLR4/NF-κB signaling pathway by downregulating the protein expression of myocardial TLR4, MyD88, and p-P65, leading to a reduction in serum pro-inflammatory factors.
36199148	7	0	theme	same	1697:1700	arg1	direction					1702:1710	the same direction	1693:1710	the same direction	1693:1710	Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy.
36199148	7	1	from	index	1684:1688	arg1	direction					1702:1710	the same direction	1693:1710	the same direction	1693:1710	Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy.
36199148	11	2	theme	microbiome-driven	2807:2823	arg1	traits					2825:2830	several microbiome-driven traits	2799:2830	several microbiome-driven traits of interest	2799:2842	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	2	3	theme	permanent	373:381	arg1	solution					400:407	a permanent and accumulative solution	371:407	a permanent and accumulative solution	371:407	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	6	4	theme	HGFC	1373:1376	arg1	influence					1378:1386	the HGFC influence	1369:1386	the HGFC influence on beef fatty acid composition	1369:1417	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	6	4	theme	HGFC	1373:1376	arg1	complex					1432:1438	complex	1432:1438	complex	1432:1438	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	1	5	theme	fatty	275:279	arg1	acids					281:285	beneficial fatty acids	264:285	beneficial fatty acids reaching host tissues	264:307	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	11	6	theme	functional	2699:2708	arg1	microbiome					2710:2719	the ruminal functional microbiome	2687:2719	the ruminal functional microbiome	2687:2719	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	5	7	from	abundances	1140:1149	arg1	rumen					1167:1171	the rumen	1163:1171	the rumen	1163:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	8	8	theme	flagellar	2025:2033	arg1	synthesis					2035:2043	flagellar synthesis	2025:2043	flagellar synthesis (flgB, fliN)	2025:2056	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	10	9	theme	methane	2507:2513	arg1	emissions					2515:2523	the environmental trait methane emissions	2483:2523	the environmental trait methane emissions (g/kg of dry matter intake)	2483:2551	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	8	10	dep	synthesis	1891:1899	arg1	aroF					1902:1905	aroF	1902:1905	aroF	1902:1905	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	10	dep	synthesis	1891:1899	arg1	serA					1911:1914	serA	1911:1914	serA	1911:1914	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	7	11	theme	CLA	1680:1682	arg1	index					1684:1688	the N3 and/or CLA index	1666:1688	the N3 and/or CLA index in the same direction	1666:1710	Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy.
36199148	4	12	theme	host	676:679	arg1	influence					689:697	host genomic influence	676:697	host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices	676:1024	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	5	13	from	rumen	1167:1171	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	13	from	rumen	1167:1171	arg1	1002/3633					1060:1068	1002/3633	1060:1068	1002/3633	1060:1068	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	13	from	rumen	1167:1171	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	13	from	rumen	1167:1171	arg1	%					1057:1057	~27.6%	1052:1057	~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1052:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	1	14	theme	adequate	194:201	arg1	manipulation					203:214	adequate manipulation	194:214	adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues	194:307	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	7	15	theme	N3	1670:1671	arg1	index					1684:1688	the N3 and/or CLA index	1666:1688	the N3 and/or CLA index in the same direction	1666:1710	Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy.
36199148	9	16	theme	selection	2262:2270	arg1	response					2272:2279	a positive selection response	2251:2279	a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity)	2251:2376	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	4	17	theme	hypercholesterolemic	925:944	arg1	C16:0					985:989	C16:0	985:989	C16:0	985:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	17	theme	hypercholesterolemic	925:944	arg1	acids					962:966	hypercholesterolemic saturated fatty acids	925:966	hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0	925:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	17	theme	hypercholesterolemic	925:944	arg1	C14:0					975:979	C14:0	975:979	C14:0	975:979	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	8	18	theme	certain	2061:2067	arg1	genera					2069:2074	certain genera	2061:2074	certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas)	2061:2133	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	4	19	theme	ruminal	641:647	arg1	mechanisms					659:668	ruminal microbial mechanisms	641:668	ruminal microbial mechanisms	641:668	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	10	20	theme	N3	2461:2462	arg1	indices					2472:2478	N3 and CLA indices	2461:2478	N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake)	2461:2551	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	9	21	theme	information	2218:2228	arg1	criteria					2230:2237	sole information criteria	2213:2237	sole information criteria	2213:2237	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	1	22	theme	rumen	223:227	arg1	microbiota					229:238	the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues	219:307	the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues	219:307	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	11	23	theme	meat	2876:2879	arg1	traits					2889:2894	meat quality traits	2876:2894	meat quality traits	2876:2894	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	4	24	theme	fatty	956:960	arg1	C16:0					985:989	C16:0	985:989	C16:0	985:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	24	theme	fatty	956:960	arg1	acids					962:966	hypercholesterolemic saturated fatty acids	925:966	hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0	925:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	24	theme	fatty	956:960	arg1	C14:0					975:979	C14:0	975:979	C14:0	975:979	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	25	theme	mechanisms	659:668	arg1	identification					623:636	a comprehensive identification	607:636	a comprehensive identification of ruminal microbial mechanisms	607:668	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	26	dep	acids	962:966	arg1	C16:0					985:989	C16:0	985:989	C16:0	985:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	26	dep	acids	962:966	arg1	acids					962:966	hypercholesterolemic saturated fatty acids	925:966	hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0	925:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	26	dep	acids	962:966	arg1	C14:0					975:979	C14:0	975:979	C14:0	975:979	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	27	dep	RESULTS	579:585	arg1	provides					598:605	provides	598:605	provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices	598:1024	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	6	28	theme	lipids	1522:1527	arg1	lipolysis					1480:1488	microbial lipolysis	1470:1488	microbial lipolysis	1470:1488	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	6	28	theme	lipids	1522:1527	arg1	biohydrogenation					1494:1509	biohydrogenation	1494:1509	biohydrogenation	1494:1509	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	8	29	dep	metabolism	1931:1940	arg1	msmX					1963:1966	msmX	1963:1966	msmX	1963:1966	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	29	dep	metabolism	1931:1940	arg1	galT					1957:1960	galT	1957:1960	galT	1957:1960	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	5	30	dep	influenced	1215:1224	arg1	HGFC					1227:1230	HGFC	1227:1230	HGFC	1227:1230	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	8	31	theme	Proteobacteria	2087:2100	arg1	phyla					2102:2106	the Proteobacteria phyla	2083:2106	the Proteobacteria phyla (e.g. Serratia, Aeromonas)	2083:2133	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	32	theme	microbial	1840:1848	arg1	genes					1850:1854	These microbial genes	1834:1854	These microbial genes	1834:1854	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	1	33	theme	BACKGROUND	136:145	arg1	ruminant					157:164	BACKGROUND Healthier ruminant	136:164	BACKGROUND Healthier ruminant products	136:173	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	4	34	from	C18:1	904:908	arg1	relation					913:920	relation	913:920	relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0	913:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	1	35	theme	ruminant	157:164	arg1	products					166:173	BACKGROUND Healthier ruminant products	136:173	BACKGROUND Healthier ruminant products	136:173	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	10	36	theme	matter	2538:2543	arg1	intake					2545:2550	dry matter intake	2534:2550	dry matter intake	2534:2550	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	4	37	dep	benefits	818:825	arg1	benefits					818:825	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	37	dep	benefits	818:825	arg1	C18:3n-3					827:834	C18:3n-3	827:834	C18:3n-3	827:834	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	37	dep	benefits	818:825	arg1	C22:6n-3					857:864	C22:6n-3	857:864	C22:6n-3	857:864	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	37	dep	benefits	818:825	arg1	cis-9					869:873	cis-9	869:873	cis-9	869:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	37	dep	benefits	818:825	arg1	C20:5n-3					837:844	C20:5n-3	837:844	C20:5n-3	837:844	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	37	dep	benefits	818:825	arg1	C22:5n-3					847:854	C22:5n-3	847:854	C22:5n-3	847:854	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	9	38	from	intensity	2367:2375	arg1	sd					2323:2324	1.36±0.24 and 0.79±0.21 sd	2299:2324	1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices	2299:2346	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	5	39	theme	additive	1094:1101	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	39	theme	additive	1094:1101	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	6	40	theme	microbial	1470:1478	arg1	lipolysis					1480:1488	microbial lipolysis	1470:1488	microbial lipolysis	1470:1488	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	0	41	theme	human	92:96	arg1	health					98:103	human health	92:103	human health	92:103	Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.
36199148	4	42	from	trans-11	876:883	arg1	relation					913:920	relation	913:920	relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0	913:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	11	43	theme	several	2799:2805	arg1	traits					2825:2830	several microbiome-driven traits	2799:2830	several microbiome-driven traits of interest	2799:2842	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	10	44	theme	strategy	2440:2447	arg1	impact					2399:2404	the impact	2395:2404	the impact of our microbiome-driven breeding strategy	2395:2447	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	11	45	theme	ruminal	2691:2697	arg1	microbiome					2710:2719	the ruminal functional microbiome	2687:2719	the ruminal functional microbiome	2687:2719	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	6	46	theme	CLA	1315:1317	arg1	n=66					1326:1329	n=66	1326:1329	n=66	1326:1329	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	6	46	theme	CLA	1315:1317	arg1	index					1319:1323	CLA index	1315:1323	CLA index (n=66)	1315:1330	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	6	46	theme	CLA	1315:1317	arg1	index					1300:1304	the N3 index	1293:1304	the N3 index (n=290)	1293:1312	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	4	47	theme	human	797:801	arg1	benefits					818:825	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	47	theme	human	797:801	arg1	C18:3n-3					827:834	C18:3n-3	827:834	C18:3n-3	827:834	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	47	theme	human	797:801	arg1	C22:6n-3					857:864	C22:6n-3	857:864	C22:6n-3	857:864	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	47	theme	human	797:801	arg1	cis-9					869:873	cis-9	869:873	cis-9	869:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	47	theme	human	797:801	arg1	C18:1					904:908	trans-11 C18:1	895:908	trans-11 C18:1	895:908	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	47	theme	human	797:801	arg1	C20:5n-3					837:844	C20:5n-3	837:844	C20:5n-3	837:844	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	47	theme	human	797:801	arg1	C22:5n-3					847:854	C22:5n-3	847:854	C22:5n-3	847:854	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	47	theme	human	797:801	arg1	trans-11					876:883	trans-11 C18:2 and trans-11 C18:1	876:908	trans-11	876:883	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	0	48	theme	methane	117:123	arg1	emissions					125:133	methane emissions	117:133	methane emissions	117:133	Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.
36199148	5	49	theme	functional	1078:1087	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	49	theme	functional	1078:1087	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	11	50	theme	interest	2835:2842	arg1	traits					2825:2830	several microbiome-driven traits	2799:2830	several microbiome-driven traits of interest	2799:2842	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	6	51	theme	index	1541:1545	arg1	variation					1547:1555	N3 index variation	1538:1555	N3 index variation	1538:1555	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	11	52	from	insight	2651:2657	arg1	possibility					2666:2676	the possibility	2662:2676	the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions	2662:2916	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	2	53	theme	interest	473:480	arg1	traits					463:468	other traits	457:468	other traits of interest (e.g. methane emissions)	457:505	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	2	53	theme	interest	473:480	arg1	emissions					496:504	methane emissions	488:504	methane emissions	488:504	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	10	54	theme	mitigation	2579:2588	arg1	response					2590:2597	a correlated mitigation response	2566:2597	a correlated mitigation response of -0.41±0.12 sd	2566:2614	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	4	55	theme	fatty	761:765	arg1	acids					767:771	unsaturated fatty acids	749:771	unsaturated fatty acids	749:771	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	10	56	dep	emissions	2515:2523	arg1	g/kg					2526:2529	g/kg	2526:2529	g/kg of dry matter intake	2526:2550	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	0	57	theme	Microbiome-driven	0:16	arg1	strategy					27:34	Microbiome-driven breeding strategy	0:34	Microbiome-driven breeding strategy	0:34	Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.
36199148	1	58	theme	host	296:299	arg1	tissues					301:307	host tissues	296:307	host tissues	296:307	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	9	59	theme	selection	2357:2365	arg1	intensity					2367:2375	2.06 selection intensity	2352:2375	2.06 selection intensity	2352:2375	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	9	60	dep	indices	2290:2296	arg1	sd					2323:2324	1.36±0.24 and 0.79±0.21 sd	2299:2324	1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices	2299:2346	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	8	61	theme	carbohydrate	1918:1929	arg1	metabolism					1931:1940	carbohydrate metabolism	1918:1940	carbohydrate metabolism	1918:1940	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	7	62	theme	microbiome-driven	1797:1813	arg1	strategy					1824:1831	a microbiome-driven breeding strategy	1795:1831	a microbiome-driven breeding strategy	1795:1831	Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy.
36199148	8	63	theme	lipopolysaccharide	1970:1987	arg1	biosynthesis					1989:2000	lipopolysaccharide biosynthesis	1970:2000	lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB)	1970:2019	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	10	64	theme	sd	2613:2614	arg1	response					2590:2597	a correlated mitigation response	2566:2597	a correlated mitigation response of -0.41±0.12 sd	2566:2614	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	9	65	theme	breeding	2156:2163	arg1	strategy					2165:2172	A microbiome-driven breeding strategy	2136:2172	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria	2136:2237	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	2	66	theme	favourable	430:439	arg1	consequences					441:452	also favourable consequences	425:452	also favourable consequences	425:452	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	5	67	theme	gene	1135:1138	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	67	theme	gene	1135:1138	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	0	68	theme	fatty	62:66	arg1	profile					73:79	beef fatty acid profile	57:79	beef fatty acid profile benefiting human health	57:103	Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.
36199148	7	69	theme	indices	1764:1770	arg1	enhancement					1744:1754	enhancement	1744:1754	enhancement of both indices	1744:1770	Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy.
36199148	4	70	theme	health	811:816	arg1	benefits					818:825	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	70	theme	health	811:816	arg1	C18:3n-3					827:834	C18:3n-3	827:834	C18:3n-3	827:834	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	70	theme	health	811:816	arg1	C22:6n-3					857:864	C22:6n-3	857:864	C22:6n-3	857:864	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	70	theme	health	811:816	arg1	cis-9					869:873	cis-9	869:873	cis-9	869:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	70	theme	health	811:816	arg1	C18:1					904:908	trans-11 C18:1	895:908	trans-11 C18:1	895:908	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	70	theme	health	811:816	arg1	C20:5n-3					837:844	C20:5n-3	837:844	C20:5n-3	837:844	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	70	theme	health	811:816	arg1	C22:5n-3					847:854	C22:5n-3	847:854	C22:5n-3	847:854	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	70	theme	health	811:816	arg1	trans-11					876:883	trans-11 C18:2 and trans-11 C18:1	876:908	trans-11	876:883	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	6	71	theme	acid	1402:1405	arg1	composition					1407:1417	beef fatty acid composition	1391:1417	beef fatty acid composition	1391:1417	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	10	72	theme	trait	2501:2505	arg1	emissions					2515:2523	the environmental trait methane emissions	2483:2523	the environmental trait methane emissions (g/kg of dry matter intake)	2483:2551	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	6	73	theme	beef	1391:1394	arg1	composition					1407:1417	beef fatty acid composition	1391:1417	beef fatty acid composition	1391:1417	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	4	74	dep	trans-11	876:883	arg1	C18:2					885:889	C18:2	885:889	C18:2	885:889	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	6	75	from	influence	1378:1386	arg1	composition					1407:1417	beef fatty acid composition	1391:1417	beef fatty acid composition	1391:1417	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	12	76	dep	Abstract	2925:2932	arg1	Video					2919:2923	Video	2919:2923	Video	2919:2923	Video Abstract.
36199148	4	77	theme	comprehensive	609:621	arg1	identification					623:636	a comprehensive identification	607:636	a comprehensive identification of ruminal microbial mechanisms	607:668	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	9	78	theme	sole	2213:2216	arg1	criteria					2230:2237	sole information criteria	2213:2237	sole information criteria	2213:2237	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	1	79	theme	beneficial	264:273	arg1	acids					281:285	beneficial fatty acids	264:285	beneficial fatty acids reaching host tissues	264:307	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	2	80	theme	Genomic	310:316	arg1	selection					318:326	Genomic selection	310:326	Genomic selection to modify the microbiome function	310:360	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	1	81	theme	acids	281:285	arg1	flux					256:259	the flux	252:259	the flux of beneficial fatty acids reaching host tissues	252:307	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	10	82	from	indices	2472:2478	arg1	emissions					2515:2523	the environmental trait methane emissions	2483:2523	the environmental trait methane emissions (g/kg of dry matter intake)	2483:2551	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	3	83	theme	data	534:537	arg1	lack					526:529	a lack	524:529	a lack of data	524:537	Possibly due to a lack of data, this strategy has never been explored.
36199148	6	84	theme	direct	1449:1454	arg1	regulation					1456:1465	the direct regulation	1445:1465	the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids	1445:1527	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	6	85	from	variations	1587:1596	arg1	HGFC					1605:1608	the HGFC	1601:1608	the HGFC than CLA	1601:1617	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	11	86	theme	host	2740:2743	arg1	selection					2753:2761	host genomic selection	2740:2761	host genomic selection	2740:2761	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	8	87	theme	protein	1883:1889	arg1	synthesis					1891:1899	microbial protein synthesis	1873:1899	microbial protein synthesis (aroF and serA)	1873:1915	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	9	88	theme	positive	2253:2260	arg1	response					2272:2279	a positive selection response	2251:2279	a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity)	2251:2376	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	5	89	theme	transformed	1113:1123	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	89	theme	transformed	1113:1123	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	4	90	theme	genomic	681:687	arg1	influence					689:697	host genomic influence	676:697	host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices	676:1024	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	9	91	dep	N3	2329:2330	arg1	indices					2340:2346	indices	2340:2346	indices	2340:2346	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	10	92	theme	dry	2534:2536	arg1	intake					2545:2550	dry matter intake	2534:2550	dry matter intake	2534:2550	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	1	93	theme	microbiota	229:238	arg1	manipulation					203:214	adequate manipulation	194:214	adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues	194:307	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	11	94	theme	quality	2881:2887	arg1	traits					2889:2894	meat quality traits	2876:2894	meat quality traits	2876:2894	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	4	95	theme	saturated	946:954	arg1	C16:0					985:989	C16:0	985:989	C16:0	985:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	95	theme	saturated	946:954	arg1	acids					962:966	hypercholesterolemic saturated fatty acids	925:966	hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0	925:989	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	95	theme	saturated	946:954	arg1	C14:0					975:979	C14:0	975:979	C14:0	975:979	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	96	theme	microbial	649:657	arg1	mechanisms					659:668	ruminal microbial mechanisms	641:668	ruminal microbial mechanisms	641:668	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	2	97	contain	have	420:423	arg2	consequences					441:452	also favourable consequences	425:452	also favourable consequences	425:452	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	2	97	contain	have	420:423	arg1	solution					400:407	a permanent and accumulative solution	371:407	a permanent and accumulative solution	371:407	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	6	98	theme	dietary	1514:1520	arg1	lipids					1522:1527	dietary lipids	1514:1527	dietary lipids	1514:1527	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	4	99	theme	trans-11	895:902	arg1	benefits					818:825	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	99	theme	trans-11	895:902	arg1	C18:1					904:908	trans-11 C18:1	895:908	trans-11 C18:1	895:908	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	9	100	theme	CLA	2336:2338	arg1	sd					2323:2324	1.36±0.24 and 0.79±0.21 sd	2299:2324	1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices	2299:2346	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	6	101	theme	biohydrogenation	1494:1509	arg1	regulation					1456:1465	the direct regulation	1445:1465	the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids	1445:1527	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	1	102	theme	Healthier	147:155	arg1	ruminant					157:164	BACKGROUND Healthier ruminant	136:164	BACKGROUND Healthier ruminant products	136:173	BACKGROUND Healthier ruminant products can be achieved by adequate manipulation of the rumen microbiota to increase the flux of beneficial fatty acids reaching host tissues.
36199148	10	103	theme	intake	2545:2550	arg1	g/kg					2526:2529	g/kg	2526:2529	g/kg of dry matter intake	2526:2550	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	8	104	theme	microbial	1873:1881	arg1	synthesis					1891:1899	microbial protein synthesis	1873:1899	microbial protein synthesis (aroF and serA)	1873:1915	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	6	105	theme	lipolysis	1480:1488	arg1	regulation					1456:1465	the direct regulation	1445:1465	the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids	1445:1527	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	4	106	from	content	738:744	arg1	beef					776:779	beef	776:779	beef	776:779	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	10	107	theme	correlated	2568:2577	arg1	response					2590:2597	a correlated mitigation response	2566:2597	a correlated mitigation response of -0.41±0.12 sd	2566:2614	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	5	108	theme	log-ratio	1103:1111	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	108	theme	log-ratio	1103:1111	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	9	109	theme	N3	2329:2330	arg1	sd					2323:2324	1.36±0.24 and 0.79±0.21 sd	2299:2324	1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices	2299:2346	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	9	110	theme	microbial	2189:2197	arg1	mechanisms					2199:2208	these microbial mechanisms	2183:2208	these microbial mechanisms as sole information criteria	2183:2237	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	5	111	theme	microbial	1125:1133	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	111	theme	microbial	1125:1133	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	2	112	dep	emissions	496:504	arg1	e.g.					483:486	e.g.	483:486	e.g.	483:486	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	10	113	theme	breeding	2431:2438	arg1	strategy					2440:2447	our microbiome-driven breeding strategy	2409:2447	our microbiome-driven breeding strategy	2409:2447	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	8	114	dep	phyla	2102:2106	arg1	Aeromonas					2124:2132	Aeromonas	2124:2132	Aeromonas	2124:2132	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	114	dep	phyla	2102:2106	arg1	Serratia					2114:2121	e.g. Serratia	2109:2121	e.g. Serratia	2109:2121	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	5	115	from	%	1057:1057	arg1	rumen					1167:1171	the rumen	1163:1171	the rumen	1163:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	0	116	theme	breeding	18:25	arg1	strategy					27:34	Microbiome-driven breeding strategy	0:34	Microbiome-driven breeding strategy	0:34	Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.
36199148	5	117	theme	core	1089:1092	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	117	theme	core	1089:1092	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	7	118	theme	breeding	1815:1822	arg1	strategy					1824:1831	a microbiome-driven breeding strategy	1795:1831	a microbiome-driven breeding strategy	1795:1831	Of these 372 alr-MG, 110 were correlated with the N3 and/or CLA index in the same direction, suggesting the opportunity for enhancement of both indices simultaneously through a microbiome-driven breeding strategy.
36199148	6	119	theme	N3	1538:1539	arg1	variation					1547:1555	N3 index variation	1538:1555	N3 index variation	1538:1555	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	11	120	dep	CONCLUSION	2617:2626	arg1	provides					2642:2649	provides	2642:2649	provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions	2642:2916	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	4	121	theme	CLA	1014:1016	arg1	indices					1018:1024	N3 and CLA indices	1007:1024	N3 and CLA indices	1007:1024	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	122	theme	unsaturated	749:759	arg1	acids					767:771	unsaturated fatty acids	749:771	unsaturated fatty acids	749:771	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	2	123	theme	other	457:461	arg1	traits					463:468	other traits	457:468	other traits of interest (e.g. methane emissions)	457:505	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	2	123	theme	other	457:461	arg1	emissions					496:504	methane emissions	488:504	methane emissions	488:504	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	10	124	theme	microbiome-driven	2413:2429	arg1	strategy					2440:2447	our microbiome-driven breeding strategy	2409:2447	our microbiome-driven breeding strategy	2409:2447	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	4	125	theme	acids	767:771	arg1	content					738:744	the content	734:744	the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices	734:1024	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	9	126	theme	microbiome-driven	2138:2154	arg1	strategy					2165:2172	A microbiome-driven breeding strategy	2136:2172	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria	2136:2237	A microbiome-driven breeding strategy based on these microbial mechanisms as sole information criteria resulted in a positive selection response for both indices (1.36±0.24 and 0.79±0.21 sd of N3 and CLA indices, at 2.06 selection intensity).
36199148	0	127	theme	beef	57:60	arg1	profile					73:79	beef fatty acid profile	57:79	beef fatty acid profile benefiting human health	57:103	Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.
36199148	2	128	theme	methane	488:494	arg1	traits					463:468	other traits	457:468	other traits of interest (e.g. methane emissions)	457:505	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	2	128	theme	methane	488:494	arg1	emissions					496:504	methane emissions	488:504	methane emissions	488:504	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	8	129	dep	biosynthesis	1989:2000	arg1	lpxB					2015:2018	lpxB	2015:2018	lpxB	2015:2018	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	129	dep	biosynthesis	1989:2000	arg1	lpxD					2009:2012	lpxD	2009:2012	lpxD	2009:2012	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	129	dep	biosynthesis	1989:2000	arg1	kdsA					2003:2006	kdsA	2003:2006	kdsA	2003:2006	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	130	dep	synthesis	2035:2043	arg1	flgB					2046:2049	flgB	2046:2049	flgB	2046:2049	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	8	130	dep	synthesis	2035:2043	arg1	fliN					2052:2055	fliN	2052:2055	fliN	2052:2055	These microbial genes were involved in microbial protein synthesis (aroF and serA), carbohydrate metabolism and transport (galT, msmX), lipopolysaccharide biosynthesis (kdsA, lpxD, lpxB), or flagellar synthesis (flgB, fliN) in certain genera within the Proteobacteria phyla (e.g. Serratia, Aeromonas).
36199148	0	131	theme	acid	68:71	arg1	profile					73:79	beef fatty acid profile	57:79	beef fatty acid profile benefiting human health	57:103	Microbiome-driven breeding strategy potentially improves beef fatty acid profile benefiting human health and reduces methane emissions.
36199148	5	132	theme	abundances	1140:1149	arg1	alr-MG					1152:1157	alr-MG	1152:1157	alr-MG	1152:1157	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	132	theme	abundances	1140:1149	arg1	1002/3633					1060:1068	1002/3633	1060:1068	1002/3633	1060:1068	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	132	theme	abundances	1140:1149	arg1	abundances					1140:1149	the functional core additive log-ratio transformed microbial gene abundances	1074:1149	the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1074:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	5	132	theme	abundances	1140:1149	arg1	%					1057:1057	~27.6%	1052:1057	~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen	1052:1171	We first identified that ~27.6% (1002/3633) of the functional core additive log-ratio transformed microbial gene abundances (alr-MG) in the rumen were at least moderately host-genomically influenced (HGFC).
36199148	4	133	theme	N3	1007:1008	arg1	indices					1018:1024	N3 and CLA indices	1007:1024	N3 and CLA indices	1007:1024	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	10	134	theme	CLA	2468:2470	arg1	indices					2472:2478	N3 and CLA indices	2461:2478	N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake)	2461:2551	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	11	135	theme	genomic	2745:2751	arg1	selection					2753:2761	host genomic selection	2740:2761	host genomic selection	2740:2761	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	6	136	theme	N3	1297:1298	arg1	index					1300:1304	the N3 index	1293:1304	the N3 index (n=290)	1293:1312	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	6	136	theme	N3	1297:1298	arg1	index					1319:1323	CLA index	1315:1323	CLA index (n=66)	1315:1330	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	6	136	theme	N3	1297:1298	arg1	n=290					1307:1311	n=290	1307:1311	n=290	1307:1311	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	4	137	theme	dietary	803:809	arg1	benefits					818:825	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9	797:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	137	theme	dietary	803:809	arg1	C18:3n-3					827:834	C18:3n-3	827:834	C18:3n-3	827:834	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	137	theme	dietary	803:809	arg1	C22:6n-3					857:864	C22:6n-3	857:864	C22:6n-3	857:864	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	137	theme	dietary	803:809	arg1	cis-9					869:873	cis-9	869:873	cis-9	869:873	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	137	theme	dietary	803:809	arg1	C18:1					904:908	trans-11 C18:1	895:908	trans-11 C18:1	895:908	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	137	theme	dietary	803:809	arg1	C20:5n-3					837:844	C20:5n-3	837:844	C20:5n-3	837:844	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	137	theme	dietary	803:809	arg1	C22:5n-3					847:854	C22:5n-3	847:854	C22:5n-3	847:854	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	4	137	theme	dietary	803:809	arg1	trans-11					876:883	trans-11 C18:2 and trans-11 C18:1	876:908	trans-11	876:883	RESULTS This study provides a comprehensive identification of ruminal microbial mechanisms under host genomic influence that directly or indirectly affect the content of unsaturated fatty acids in beef associated with human dietary health benefits C18:3n-3, C20:5n-3, C22:5n-3, C22:6n-3 or cis-9, trans-11 C18:2 and trans-11 C18:1 in relation to hypercholesterolemic saturated fatty acids C12:0, C14:0 and C16:0, referred to as N3 and CLA indices.
36199148	2	138	theme	microbiome	342:351	arg1	function					353:360	the microbiome function	338:360	the microbiome function	338:360	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
36199148	6	139	theme	fatty	1396:1400	arg1	composition					1407:1417	beef fatty acid composition	1391:1417	beef fatty acid composition	1391:1417	Of these, 372 alr-MG were host-genomically correlated with the N3 index (n=290), CLA index (n=66) or with both (n=16), indicating that the HGFC influence on beef fatty acid composition is much more complex than the direct regulation of microbial lipolysis and biohydrogenation of dietary lipids and that N3 index variation is more strongly subjected to variations in the HGFC than CLA.
36199148	11	140	theme	methane	2900:2906	arg1	emissions					2908:2916	methane emissions	2900:2916	methane emissions	2900:2916	CONCLUSION This research provides insight on the possibility of using the ruminal functional microbiome as information for host genomic selection, which could simultaneously improve several microbiome-driven traits of interest, in this study exemplified with meat quality traits and methane emissions.
36199148	10	141	theme	environmental	2487:2499	arg1	emissions					2515:2523	the environmental trait methane emissions	2483:2523	the environmental trait methane emissions (g/kg of dry matter intake)	2483:2551	When evaluating the impact of our microbiome-driven breeding strategy to increase N3 and CLA indices on the environmental trait methane emissions (g/kg of dry matter intake), we obtained a correlated mitigation response of -0.41±0.12 sd.
36199148	2	142	theme	accumulative	387:398	arg1	solution					400:407	a permanent and accumulative solution	371:407	a permanent and accumulative solution	371:407	Genomic selection to modify the microbiome function provides a permanent and accumulative solution, which may have also favourable consequences in other traits of interest (e.g. methane emissions).
35143514	10	0	theme	optical	1442:1448	arg1	FRAP					1409:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	10	0	theme	optical	1442:1448	arg1	method					1450:1455	a feasible and practical optical method	1417:1455	a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous	1417:1516	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	9	1	theme	±standard	1218:1226	arg1	viscosity					1239:1247	The mean (±standard deviation) viscosity	1208:1247	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA	1200:1297	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	8	2	theme	bleach	1163:1168	arg1	areas					1170:1174	FRAP bleach areas	1158:1174	FRAP bleach areas of different diameters	1158:1197	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	10	3	theme	macromolecules	1486:1499	arg1	diffusion					1473:1481	the diffusion	1469:1481	the diffusion of macromolecules	1469:1499	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	10	4	theme	practical	1432:1440	arg1	FRAP					1409:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	10	4	theme	practical	1432:1440	arg1	method					1450:1455	a feasible and practical optical method	1417:1455	a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous	1417:1516	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	9	5	theme	deviation	1228:1236	arg1	viscosity					1239:1247	The mean (±standard deviation) viscosity	1208:1247	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA	1200:1297	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	9	6	theme	porcine	1252:1258	arg1	vitreous					1260:1267	porcine vitreous	1252:1267	porcine vitreous using dextran, ficoll and BSA	1252:1297	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	9	7	dep	1.40	1311:1314	arg1	cP					1345:1346	0.13 cP	1340:1346	0.13 cP	1340:1346	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	3	8	theme	vitreous	370:377	arg1	viscosity					379:387	human vitreous viscosity	364:387	human vitreous viscosity	364:387	The central pig vitreous is thought to closely match human vitreous viscosity.
35143514	8	9	theme	molecular	1077:1085	arg1	weights					1087:1093	varying molecular weights	1069:1093	varying molecular weights (MWs)	1069:1099	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	9	theme	molecular	1077:1085	arg1	MWs					1096:1098	MWs	1096:1098	MWs	1096:1098	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	10	theme	FITC-bovine	1106:1116	arg1	albumin					1124:1130	FITC-bovine serum albumin	1106:1130	FITC-bovine serum albumin (BSA)	1106:1136	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	10	theme	FITC-bovine	1106:1116	arg1	BSA					1133:1135	BSA	1133:1135	BSA	1133:1135	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	7	11	theme	fluorescence	949:960	arg1	recovery					962:969	fluorescence recovery	949:969	fluorescence recovery	949:969	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	12	dep	METHODS	799:805	arg1	viscosity					816:824	Vitreous viscosity	807:824	Vitreous viscosity	807:824	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	12	dep	METHODS	799:805	arg1	METHODS					799:805	METHODS	799:805	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules	799:907	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	12	dep	METHODS	799:805	arg1	diffusion					858:866	intravitreal macromolecular diffusion	830:866	intravitreal macromolecular diffusion	830:866	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	1	13	theme	PURPOSE	81:87	arg1	humor					98:102	PURPOSE Vitreous humor	81:102	PURPOSE Vitreous humor	81:102	PURPOSE Vitreous humor is a complex biofluid whose composition determines its structure and function.
35143514	1	13	theme	PURPOSE	81:87	arg1	biofluid					117:124	a complex biofluid	107:124	a complex biofluid whose composition determines its structure and function	107:180	PURPOSE Vitreous humor is a complex biofluid whose composition determines its structure and function.
35143514	8	14	theme	varying	1069:1075	arg1	weights					1087:1093	varying molecular weights	1069:1093	varying molecular weights (MWs)	1069:1099	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	14	theme	varying	1069:1075	arg1	MWs					1096:1098	MWs	1096:1098	MWs	1096:1098	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	2	15	theme	molecules	300:308	arg1	half-life					246:254	half-life	246:254	half-life	246:254	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	2	15	theme	molecules	300:308	arg1	delivery					218:225	delivery	218:225	delivery	218:225	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	2	15	theme	molecules	300:308	arg1	distribution					228:239	distribution	228:239	distribution	228:239	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	7	16	theme	porcine	930:936	arg1	eyes					938:941	porcine eyes	930:941	porcine eyes	930:941	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	4	17	theme	biochemical	486:496	arg1	reactions					498:506	all biochemical reactions	482:506	all biochemical reactions	482:506	Diffusion is inversely related to viscosity, and diffusion is of fundamental importance for all biochemical reactions.
35143514	1	18	theme	Vitreous	89:96	arg1	humor					98:102	PURPOSE Vitreous humor	81:102	PURPOSE Vitreous humor	81:102	PURPOSE Vitreous humor is a complex biofluid whose composition determines its structure and function.
35143514	1	18	theme	Vitreous	89:96	arg1	biofluid					117:124	a complex biofluid	107:124	a complex biofluid whose composition determines its structure and function	107:180	PURPOSE Vitreous humor is a complex biofluid whose composition determines its structure and function.
35143514	9	19	dep	RESULTS	1200:1206	arg1	viscosity					1239:1247	The mean (±standard deviation) viscosity	1208:1247	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA	1200:1297	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	3	20	theme	human	364:368	arg1	viscosity					379:387	human vitreous viscosity	364:387	human vitreous viscosity	364:387	The central pig vitreous is thought to closely match human vitreous viscosity.
35143514	8	21	theme	serum	1118:1122	arg1	albumin					1124:1130	FITC-bovine serum albumin	1106:1130	FITC-bovine serum albumin (BSA)	1106:1136	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	21	theme	serum	1118:1122	arg1	BSA					1133:1135	BSA	1133:1135	BSA	1133:1135	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	22	theme	weights	1087:1093	arg1	ficolls					1058:1064	ficolls	1058:1064	ficolls	1058:1064	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	22	theme	weights	1087:1093	arg1	dextrans					1045:1052	(FITC) dextrans	1038:1052	(FITC) dextrans	1038:1052	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	2	23	theme	key	282:284	arg1	molecules					300:308	key physiological molecules	282:308	key physiological molecules	282:308	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	0	24	theme	vitreous	12:19	arg1	viscosity					21:29	vitreous viscosity	12:29	vitreous viscosity	12:29	Determining vitreous viscosity using fluorescence recovery after photobleaching.
35143514	5	25	theme	physiological	656:668	arg1	macromolecules					670:683	physiological macromolecules	656:683	physiological macromolecules	656:683	Fluorescence Recovery After Photobleaching (FRAP) may provide a novel means of measuring intravitreal diffusion that could be applied to drugs and physiological macromolecules.
35143514	5	26	theme	intravitreal	598:609	arg1	diffusion					611:619	intravitreal diffusion	598:619	intravitreal diffusion that could be applied to drugs and physiological macromolecules	598:683	Fluorescence Recovery After Photobleaching (FRAP) may provide a novel means of measuring intravitreal diffusion that could be applied to drugs and physiological macromolecules.
35143514	6	27	theme	vitreous	726:733	arg1	viscosity					735:743	vitreous viscosity	726:743	vitreous viscosity	726:743	It would also provide information about vitreous viscosity, which is relevant to drug elimination, and delivery.
35143514	10	28	theme	CONCLUSIONS	1397:1407	arg1	FRAP					1409:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	10	28	theme	CONCLUSIONS	1397:1407	arg1	method					1450:1455	a feasible and practical optical method	1417:1455	a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous	1417:1516	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	1	29	theme	complex	109:115	arg1	humor					98:102	PURPOSE Vitreous humor	81:102	PURPOSE Vitreous humor	81:102	PURPOSE Vitreous humor is a complex biofluid whose composition determines its structure and function.
35143514	1	29	theme	complex	109:115	arg1	biofluid					117:124	a complex biofluid	107:124	a complex biofluid whose composition determines its structure and function	107:180	PURPOSE Vitreous humor is a complex biofluid whose composition determines its structure and function.
35143514	9	30	theme	mean	1212:1215	arg1	viscosity					1239:1247	The mean (±standard deviation) viscosity	1208:1247	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA	1200:1297	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	7	31	theme	intravitreal	830:841	arg1	METHODS					799:805	METHODS	799:805	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules	799:907	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	31	theme	intravitreal	830:841	arg1	diffusion					858:866	intravitreal macromolecular diffusion	830:866	intravitreal macromolecular diffusion	830:866	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	2	32	theme	physiological	286:298	arg1	molecules					300:308	key physiological molecules	282:308	key physiological molecules	282:308	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	7	33	theme	labelled	885:892	arg1	macromolecules					894:907	fluorescently labelled macromolecules	871:907	fluorescently labelled macromolecules	871:907	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	34	theme	macromolecules	894:907	arg1	viscosity					816:824	Vitreous viscosity	807:824	Vitreous viscosity	807:824	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	34	theme	macromolecules	894:907	arg1	METHODS					799:805	METHODS	799:805	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules	799:907	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	34	theme	macromolecules	894:907	arg1	diffusion					858:866	intravitreal macromolecular diffusion	830:866	intravitreal macromolecular diffusion	830:866	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	35	theme	macromolecular	843:856	arg1	METHODS					799:805	METHODS	799:805	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules	799:907	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	35	theme	macromolecular	843:856	arg1	diffusion					858:866	intravitreal macromolecular diffusion	830:866	intravitreal macromolecular diffusion	830:866	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	4	36	theme	fundamental	455:465	arg1	importance					467:476	fundamental importance	455:476	fundamental importance	455:476	Diffusion is inversely related to viscosity, and diffusion is of fundamental importance for all biochemical reactions.
35143514	5	37	theme	Fluorescence	509:520	arg1	Recovery					522:529	Fluorescence Recovery	509:529	Fluorescence Recovery After Photobleaching (FRAP)	509:557	Fluorescence Recovery After Photobleaching (FRAP) may provide a novel means of measuring intravitreal diffusion that could be applied to drugs and physiological macromolecules.
35143514	0	38	theme	fluorescence	37:48	arg1	recovery					50:57	fluorescence recovery	37:57	fluorescence recovery	37:57	Determining vitreous viscosity using fluorescence recovery after photobleaching.
35143514	8	39	theme	Fluorescein	1000:1010	arg1	isothiocyanate					1012:1025	Fluorescein isothiocyanate	1000:1025	Fluorescein isothiocyanate	1000:1025	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	10	40	theme	feasible	1419:1426	arg1	FRAP					1409:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP	1397:1412	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	10	40	theme	feasible	1419:1426	arg1	method					1450:1455	a feasible and practical optical method	1417:1455	a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous	1417:1516	CONCLUSIONS FRAP is a feasible and practical optical method to quantify the diffusion of macromolecules through vitreous.
35143514	3	41	theme	pig	323:325	arg1	vitreous					327:334	The central pig vitreous	311:334	The central pig vitreous	311:334	The central pig vitreous is thought to closely match human vitreous viscosity.
35143514	2	42	theme	Vitreous	183:190	arg1	viscosity					192:200	Vitreous viscosity	183:200	Vitreous viscosity	183:200	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	5	43	theme	novel	573:577	arg1	means					579:583	a novel means	571:583	a novel means of measuring intravitreal diffusion that could be applied to drugs and physiological macromolecules	571:683	Fluorescence Recovery After Photobleaching (FRAP) may provide a novel means of measuring intravitreal diffusion that could be applied to drugs and physiological macromolecules.
35143514	8	44	theme	FRAP	1158:1161	arg1	areas					1170:1174	FRAP bleach areas	1158:1174	FRAP bleach areas of different diameters	1158:1197	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	7	45	theme	Vitreous	807:814	arg1	viscosity					816:824	Vitreous viscosity	807:824	Vitreous viscosity	807:824	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	7	45	theme	Vitreous	807:814	arg1	METHODS					799:805	METHODS	799:805	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules	799:907	METHODS Vitreous viscosity and intravitreal macromolecular diffusion of fluorescently labelled macromolecules were investigated in porcine eyes using fluorescence recovery after photobleaching (FRAP).
35143514	9	46	theme	3.54	1304:1307	arg1	±					1309:1309	±	1309:1309	±	1309:1309	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	2	47	dep	delivery	218:225	arg1	the					214:216	the	214:216	the	214:216	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	3	48	theme	central	315:321	arg1	vitreous					327:334	The central pig vitreous	311:334	The central pig vitreous	311:334	The central pig vitreous is thought to closely match human vitreous viscosity.
35143514	8	49	theme	FITC	1039:1042	arg1	dextrans					1045:1052	(FITC) dextrans	1038:1052	(FITC) dextrans	1038:1052	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	8	50	theme	diameters	1189:1197	arg1	areas					1170:1174	FRAP bleach areas	1158:1174	FRAP bleach areas of different diameters	1158:1197	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	2	51	theme	drugs	271:275	arg1	half-life					246:254	half-life	246:254	half-life	246:254	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	2	51	theme	drugs	271:275	arg1	delivery					218:225	delivery	218:225	delivery	218:225	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	2	51	theme	drugs	271:275	arg1	distribution					228:239	distribution	228:239	distribution	228:239	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	6	52	theme	drug	767:770	arg1	elimination					772:782	drug elimination	767:782	drug elimination	767:782	It would also provide information about vitreous viscosity, which is relevant to drug elimination, and delivery.
35143514	9	53	theme	cP	1393:1394	arg1	average					1370:1376	an average	1367:1376	an average of 3.65 ± 0.60 cP	1367:1394	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
35143514	8	54	theme	different	1179:1187	arg1	diameters					1189:1197	different diameters	1179:1197	different diameters	1179:1197	Fluorescein isothiocyanate conjugated (FITC) dextrans and ficolls of varying molecular weights (MWs), and FITC-bovine serum albumin (BSA) were employed using FRAP bleach areas of different diameters.
35143514	2	55	theme	intraocular	259:269	arg1	drugs					271:275	intraocular drugs	259:275	intraocular drugs	259:275	Vitreous viscosity will affect the delivery, distribution, and half-life of intraocular drugs, and key physiological molecules.
35143514	9	56	theme	vitreous	1260:1267	arg1	viscosity					1239:1247	The mean (±standard deviation) viscosity	1208:1247	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA	1200:1297	RESULTS The mean (±standard deviation) viscosity of porcine vitreous using dextran, ficoll and BSA were 3.54 ± 1.40, 2.86 ± 1.13 and 4.54 ± 0.13 cP respectively, with an average of 3.65 ± 0.60 cP.
36361674	2	0	from	causes	181:186	arg1	world					208:212	the world	204:212	the world	204:212	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	9	1	theme	invasive	1385:1392	arg1	MDA-MB-231					1394:1403	highly invasive MDA-MB-231	1378:1403	highly invasive MDA-MB-231	1378:1403	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	10	2	theme	nitric	1553:1558	arg1	NO					1567:1568	NO	1567:1568	NO	1567:1568	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	10	2	theme	nitric	1553:1558	arg1	oxide					1560:1564	nitric oxide	1553:1564	nitric oxide (NO) production	1553:1580	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	5	3	theme	multi-target	611:622	arg1	potential					635:643	the multi-target biological potential	607:643	the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed	607:850	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	10	4	theme	RAW	1738:1740	arg1	cells					1748:1752	RAW 264.7 cells	1738:1752	RAW 264.7 cells	1738:1752	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	1	5	theme	LPS-Stimulated	120:133	arg1	Cells					145:149	LPS-Stimulated RAW 264.7 Cells	120:149	LPS-Stimulated RAW 264.7 Cells	120:149	Exerts Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects in LPS-Stimulated RAW 264.7 Cells.
36361674	7	6	theme	Anti-proliferative	1064:1081	arg1	activity					1083:1090	Anti-proliferative activity	1064:1090	Anti-proliferative activity	1064:1090	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	0	7	theme	Santolina	0:8	arg1	Viv					18:20	Santolina pinnata Viv	0:20	Santolina pinnata Viv.	0:21	Santolina pinnata Viv.
36361674	6	8	theme	n-Hexane	853:860	arg1	extracts					889:896	n-Hexane (EHSP) and methanol (EMSP) extracts	853:896	n-Hexane (EHSP) and methanol (EMSP) extracts	853:896	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	5	9	with	endowed	779:785	arg1	features					825:832	anticancer and anti-inflammatory features	792:832	anticancer and anti-inflammatory features	792:832	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	5	10	theme	first	595:599	arg1	time					601:604	the first time	591:604	the first time	591:604	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	14	11	theme	different	2297:2305	arg1	related					2365:2371	related	2365:2371	related	2365:2371	The different biological behaviours found between the extracts could be related to their different chemical compositions.
36361674	14	11	theme	different	2297:2305	arg1	behaviours					2318:2327	The different biological behaviours	2293:2327	The different biological behaviours found between the extracts	2293:2354	The different biological behaviours found between the extracts could be related to their different chemical compositions.
36361674	12	12	theme	μg/mL	1962:1966	arg1	activity					1938:1945	The highest activity	1926:1945	The highest activity (IC50 of 15.91 μg/mL)	1926:1967	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	12	12	theme	μg/mL	1962:1966	arg1	IC50					1948:1951	IC50	1948:1951	IC50 of 15.91 μg/mL	1948:1966	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	16	13	theme	important	2589:2597	arg1	stepping-stones					2599:2613	important stepping-stones	2589:2613	important stepping-stones for further investigations	2589:2640	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	11	14	theme	EMSP	1764:1767	arg1	extracts					1769:1776	EHSP and EMSP extracts	1755:1776	EHSP and EMSP extracts	1755:1776	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	5	15	theme	pinnata	666:672	arg1	potential					635:643	the multi-target biological potential	607:643	the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed	607:850	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	2	16	theme	anticancer	264:273	arg1	drugs					275:279	novel anticancer drugs	258:279	novel anticancer drugs	258:279	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	12	17	theme	highest	1930:1936	arg1	activity					1938:1945	The highest activity	1926:1945	The highest activity (IC50 of 15.91 μg/mL)	1926:1967	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	12	17	theme	highest	1930:1936	arg1	IC50					1948:1951	IC50	1948:1951	IC50 of 15.91 μg/mL	1948:1966	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	9	18	theme	cell	1462:1465	arg1	lines					1467:1471	both breast cancer cell lines	1443:1471	both breast cancer cell lines	1443:1471	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	13	19	dep	61.14	2151:2155	arg1	to					2148:2149	to	2148:2149	to	2148:2149	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	10	20	theme	nuclear	1590:1596	arg1	kappa-light-chain-enhancer					1605:1630	the nuclear factor kappa-light-chain-enhancer	1586:1630	the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)	1586:1659	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	10	20	theme	nuclear	1590:1596	arg1	NF-κB					1654:1658	NF-κB	1654:1658	NF-κB	1654:1658	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	11	21	theme	breast	1816:1821	arg1	cells					1830:1834	breast cancer cells	1816:1834	breast cancer cells	1816:1834	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	13	22	theme	IC50	2118:2121	arg1	values					2123:2128	IC50 values	2118:2128	IC50 values ranging from 27.5 to 61.14 μg/mL	2118:2161	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	13	23	theme	anti-inflammatory	2091:2107	arg1	effects					2109:2115	anti-inflammatory effects	2091:2115	anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL)	2091:2162	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	9	24	theme	breast	1448:1453	arg1	lines					1467:1471	both breast cancer cell lines	1443:1471	both breast cancer cell lines	1443:1471	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	3	25	attach	derived	369:375	arg2	drugs					332:336	Many successful anticancer drugs	305:336	Many successful anticancer drugs used in clinical practices	305:363	Many successful anticancer drugs used in clinical practices are derived from natural products.
36361674	3	25	attach	derived	369:375	arg1	products					390:397	natural products	382:397	natural products	382:397	Many successful anticancer drugs used in clinical practices are derived from natural products.
36361674	15	26	theme	multi-target	2427:2438	arg1	potential					2451:2459	the multi-target biological potential	2423:2459	the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects	2423:2525	Herein, the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects was comprehensively demonstrated.
36361674	1	27	theme	RAW	135:137	arg1	Cells					145:149	LPS-Stimulated RAW 264.7 Cells	120:149	LPS-Stimulated RAW 264.7 Cells	120:149	Exerts Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects in LPS-Stimulated RAW 264.7 Cells.
36361674	5	28	theme	Italian	648:654	arg1	pinnata					666:672	Italian Santolina pinnata	648:672	Italian Santolina pinnata	648:672	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	13	29	theme	NF-κB	2230:2234	arg1	translocation					2244:2256	NF-κB nuclear translocation	2230:2256	NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells	2230:2290	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	4	30	theme	genus	404:408	arg1	species					434:440	species	434:440	species distributed in the Mediterranean area and used in traditional medicine for their biological properties	434:543	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	4	30	theme	genus	404:408	arg1	Santolina					410:418	The genus Santolina	400:418	The genus Santolina	400:418	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	4	30	theme	genus	404:408	arg1	group					425:429	a group	423:429	a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties	423:543	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	12	31	theme	invasive	2017:2024	arg1	line					2045:2048	a highly invasive breast cancer cell line	2008:2048	a highly invasive breast cancer cell line	2008:2048	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	12	31	theme	invasive	2017:2024	arg1	cells					2001:2005	MDA-MB-231 cells	1990:2005	MDA-MB-231 cells	1990:2005	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	9	32	theme	gel-zymography	1477:1490	arg1	experiments					1492:1502	gel-zymography experiments	1477:1502	gel-zymography experiments	1477:1502	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	6	33	theme	chromatography-mass	951:969	arg1	spectrometry					971:982	gas chromatography-mass spectrometry	947:982	gas chromatography-mass spectrometry	947:982	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	4	34	theme	Mediterranean	461:473	arg1	area					475:478	the Mediterranean area	457:478	the Mediterranean area	457:478	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	12	35	theme	cancer	2033:2038	arg1	line					2045:2048	a highly invasive breast cancer cell line	2008:2048	a highly invasive breast cancer cell line	2008:2048	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	12	35	theme	cancer	2033:2038	arg1	cells					2001:2005	MDA-MB-231 cells	1990:2005	MDA-MB-231 cells	1990:2005	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	11	36	theme	EHSP	1755:1758	arg1	extracts					1769:1776	EHSP and EMSP extracts	1755:1776	EHSP and EMSP extracts	1755:1776	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	11	37	dep	exhibited	1778:1786	arg1	promoting					1837:1845	promoting	1837:1845	promoting apoptotic death	1837:1861	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	11	37	dep	exhibited	1778:1786	arg1	decreasing					1875:1884	decreasing	1875:1884	decreasing cell migration and invasive behaviours	1875:1923	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	5	38	theme	work	562:565	arg1	aim					550:552	The aim	546:552	The aim of this work	546:565	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	8	39	theme	comet	1312:1316	arg1	assay					1318:1322	comet assay	1312:1322	comet assay	1312:1322	Apoptotic death was assessed by comet assay.
36361674	7	40	theme	non-tumorigenic	1164:1178	arg1	cells					1188:1192	non-tumorigenic MCF-10A cells	1164:1192	non-tumorigenic MCF-10A cells	1164:1192	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	15	41	theme	antitumor	2487:2495	arg1	effects					2519:2525	antitumor and anti-inflammatory effects	2487:2525	antitumor and anti-inflammatory effects	2487:2525	Herein, the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects was comprehensively demonstrated.
36361674	1	42	theme	Breast	67:72	arg1	Cells					81:85	Breast Cancer Cells	67:85	Breast Cancer Cells	67:85	Exerts Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects in LPS-Stimulated RAW 264.7 Cells.
36361674	3	43	theme	successful	310:319	arg1	drugs					332:336	Many successful anticancer drugs	305:336	Many successful anticancer drugs used in clinical practices	305:363	Many successful anticancer drugs used in clinical practices are derived from natural products.
36361674	4	44	theme	species	434:440	arg1	species					434:440	species	434:440	species distributed in the Mediterranean area and used in traditional medicine for their biological properties	434:543	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	4	44	theme	species	434:440	arg1	Santolina					410:418	The genus Santolina	400:418	The genus Santolina	400:418	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	4	44	theme	species	434:440	arg1	group					425:429	a group	423:429	a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties	423:543	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	7	45	dep	MCF-7	1108:1112	arg1	cells					1143:1147	breast cancer cells	1129:1147	breast cancer cells	1129:1147	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	11	46	theme	cell	1886:1889	arg1	migration					1891:1899	cell migration	1886:1899	cell migration	1886:1899	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	16	47	theme	extract-based	2705:2717	arg1	treatments					2719:2728	highly effective S. pinnata extract-based treatments	2677:2728	highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes	2677:2773	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	5	48	theme	phytochemicals	764:777	arg1	source					745:750	an interesting natural source	722:750	an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features	722:832	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	1	49	theme	Anti-Inflammatory	91:107	arg1	Effects					109:115	Anti-Inflammatory Effects	91:115	Anti-Inflammatory Effects	91:115	Exerts Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects in LPS-Stimulated RAW 264.7 Cells.
36361674	16	50	dep	S.	2694:2695	arg1	pinnata					2697:2703	pinnata	2697:2703	pinnata	2697:2703	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	6	51	theme	ultra-high-performance	996:1017	arg1	UHPLC					1042:1046	UHPLC	1042:1046	UHPLC	1042:1046	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	6	51	theme	ultra-high-performance	996:1017	arg1	chromatography					1026:1039	ultra-high-performance liquid chromatography	996:1039	ultra-high-performance liquid chromatography (UHPLC)	996:1047	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	3	52	theme	natural	382:388	arg1	products					390:397	natural products	382:397	natural products	382:397	Many successful anticancer drugs used in clinical practices are derived from natural products.
36361674	2	53	theme	largest	173:179	arg1	causes					181:186	the largest causes	169:186	the largest causes of mortality in the world	169:212	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	5	54	theme	natural	737:743	arg1	source					745:750	an interesting natural source	722:750	an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features	722:832	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	9	55	theme	Cell	1325:1328	arg1	motility					1330:1337	Cell motility	1325:1337	Cell motility	1325:1337	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	7	56	theme	breast	1129:1134	arg1	cells					1143:1147	breast cancer cells	1129:1147	breast cancer cells	1129:1147	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	16	57	theme	inflammatory	2752:2763	arg1	processes					2765:2773	inflammatory processes	2752:2773	inflammatory processes	2752:2773	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	10	58	theme	bacterial	1685:1693	arg1	LPS					1716:1718	LPS	1716:1718	LPS	1716:1718	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	10	58	theme	bacterial	1685:1693	arg1	lipopolysaccharides					1695:1713	bacterial lipopolysaccharides	1685:1713	bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells	1685:1752	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	13	59	from	translocation	2244:2256	arg1	cells					2286:2290	LPS-stimulated RAW 264.7 cells	2261:2290	LPS-stimulated RAW 264.7 cells	2261:2290	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	1	60	theme	Promising	30:38	arg1	Activity					50:57	Promising Antitumor Activity	30:57	Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects	30:115	Exerts Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects in LPS-Stimulated RAW 264.7 Cells.
36361674	7	61	theme	-2,5-diphenyl-2H-tetrazolium	1230:1257	arg1	assay					1273:1277	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay	1198:1277	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay	1198:1277	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	13	62	dep	effects	2109:2115	arg1	values					2123:2128	IC50 values	2118:2128	IC50 values ranging from 27.5 to 61.14 μg/mL	2118:2161	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	11	63	theme	invasive	1905:1912	arg1	behaviours					1914:1923	invasive behaviours	1905:1923	invasive behaviours	1905:1923	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	12	64	theme	MDA-MB-231	1990:1999	arg1	cells					2001:2005	MDA-MB-231 cells	1990:2005	MDA-MB-231 cells	1990:2005	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	12	64	theme	MDA-MB-231	1990:1999	arg1	line					2045:2048	a highly invasive breast cancer cell line	2008:2048	a highly invasive breast cancer cell line	2008:2048	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	10	65	theme	anti-inflammatory	1509:1525	arg1	potential					1527:1535	The anti-inflammatory potential	1505:1535	The anti-inflammatory potential	1505:1535	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	6	66	theme	methanol	873:880	arg1	extracts					889:896	n-Hexane (EHSP) and methanol (EMSP) extracts	853:896	n-Hexane (EHSP) and methanol (EMSP) extracts	853:896	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	13	67	dep	able	2075:2078	arg1	promote					2083:2089	promote	2083:2089	to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL)	2080:2162	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	13	67	dep	able	2075:2078	arg1	reduce					2179:2184	reduce	2179:2184	to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells	2176:2290	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	10	68	theme	oxide	1560:1564	arg1	production					1571:1580	nitric oxide (NO) production	1553:1580	nitric oxide (NO) production	1553:1580	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	2	69	theme	novel	258:262	arg1	drugs					275:279	novel anticancer drugs	258:279	novel anticancer drugs	258:279	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	5	70	dep	phytochemicals	764:777	arg1	endowed					779:785	endowed	779:785	endowed	779:785	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	9	71	theme	wound-healing	1408:1420	arg1	scratches					1422:1430	wound-healing scratches	1408:1430	wound-healing scratches	1408:1430	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	13	72	theme	RAW	2276:2278	arg1	cells					2286:2290	LPS-stimulated RAW 264.7 cells	2261:2290	LPS-stimulated RAW 264.7 cells	2261:2290	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	14	73	theme	biological	2307:2316	arg1	related					2365:2371	related	2365:2371	related	2365:2371	The different biological behaviours found between the extracts could be related to their different chemical compositions.
36361674	14	73	theme	biological	2307:2316	arg1	behaviours					2318:2327	The different biological behaviours	2293:2327	The different biological behaviours found between the extracts	2293:2354	The different biological behaviours found between the extracts could be related to their different chemical compositions.
36361674	11	74	theme	anticancer	1788:1797	arg1	activity					1799:1806	anticancer activity	1788:1806	anticancer activity against breast cancer cells	1788:1834	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	5	75	theme	Santolina	656:664	arg1	pinnata					666:672	Italian Santolina pinnata	648:672	Italian Santolina pinnata	648:672	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	2	76	theme	drugs	275:279	arg1	discovery					245:253	the discovery	241:253	the discovery of novel anticancer drugs	241:279	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	15	77	theme	anti-inflammatory	2501:2517	arg1	effects					2519:2525	antitumor and anti-inflammatory effects	2487:2525	antitumor and anti-inflammatory effects	2487:2525	Herein, the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects was comprehensively demonstrated.
36361674	5	78	theme	anticancer	792:801	arg1	features					825:832	anticancer and anti-inflammatory features	792:832	anticancer and anti-inflammatory features	792:832	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	12	79	theme	15.91	1956:1960	arg1	μg/mL					1962:1966	15.91 μg/mL	1956:1966	15.91 μg/mL	1956:1966	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	6	80	theme	gas	919:921	arg1	GC					939:940	GC	939:940	GC	939:940	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	6	80	theme	gas	919:921	arg1	chromatography					923:936	gas chromatography	919:936	gas chromatography (GC)	919:941	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	6	80	theme	gas	919:921	arg1	GC-MS					985:989	GC-MS	985:989	GC-MS	985:989	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	10	81	theme	factor	1598:1603	arg1	kappa-light-chain-enhancer					1605:1630	the nuclear factor kappa-light-chain-enhancer	1586:1630	the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)	1586:1659	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	10	81	theme	factor	1598:1603	arg1	NF-κB					1654:1658	NF-κB	1654:1658	NF-κB	1654:1658	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	5	82	theme	anti-inflammatory	807:823	arg1	features					825:832	anticancer and anti-inflammatory features	792:832	anticancer and anti-inflammatory features	792:832	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	11	83	theme	cancer	1823:1828	arg1	cells					1830:1834	breast cancer cells	1816:1834	breast cancer cells	1816:1834	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	5	84	theme	biological	624:633	arg1	potential					635:643	the multi-target biological potential	607:643	the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed	607:850	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	9	85	theme	cancer	1455:1460	arg1	lines					1467:1471	both breast cancer cell lines	1443:1471	both breast cancer cell lines	1443:1471	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	12	86	theme	cell	2040:2043	arg1	line					2045:2048	a highly invasive breast cancer cell line	2008:2048	a highly invasive breast cancer cell line	2008:2048	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	12	86	theme	cell	2040:2043	arg1	cells					2001:2005	MDA-MB-231 cells	1990:2005	MDA-MB-231 cells	1990:2005	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	15	87	theme	biological	2440:2449	arg1	potential					2451:2459	the multi-target biological potential	2423:2459	the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects	2423:2525	Herein, the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects was comprehensively demonstrated.
36361674	16	88	dep	effective	2684:2692	arg1	S.					2694:2695	S.	2694:2695	S.	2694:2695	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	14	89	dep	different	2382:2390	arg1	chemical					2392:2399	chemical	2392:2399	chemical	2392:2399	The different biological behaviours found between the extracts could be related to their different chemical compositions.
36361674	13	90	theme	nuclear	2236:2242	arg1	translocation					2244:2256	NF-κB nuclear translocation	2230:2256	NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells	2230:2290	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	3	91	theme	Many	305:308	arg1	drugs					332:336	Many successful anticancer drugs	305:336	Many successful anticancer drugs used in clinical practices	305:363	Many successful anticancer drugs used in clinical practices are derived from natural products.
36361674	8	92	theme	Apoptotic	1280:1288	arg1	death					1290:1294	Apoptotic death	1280:1294	Apoptotic death	1280:1294	Apoptotic death was assessed by comet assay.
36361674	7	93	theme	MCF-10A	1180:1186	arg1	cells					1188:1192	non-tumorigenic MCF-10A cells	1164:1192	non-tumorigenic MCF-10A cells	1164:1192	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	16	94	theme	further	2619:2625	arg1	investigations					2627:2640	further investigations	2619:2640	further investigations	2619:2640	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	6	95	theme	gas	947:949	arg1	spectrometry					971:982	gas chromatography-mass spectrometry	947:982	gas chromatography-mass spectrometry	947:982	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	12	96	theme	breast	2026:2031	arg1	line					2045:2048	a highly invasive breast cancer cell line	2008:2048	a highly invasive breast cancer cell line	2008:2048	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	12	96	theme	breast	2026:2031	arg1	cells					2001:2005	MDA-MB-231 cells	1990:2005	MDA-MB-231 cells	1990:2005	The highest activity (IC50 of 15.91 μg/mL) was detected against MDA-MB-231 cells, a highly invasive breast cancer cell line.
36361674	5	97	theme	chemical	695:702	arg1	profile					704:710	their chemical profile	689:710	their chemical profile	689:710	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	1	98	theme	Cancer	74:79	arg1	Cells					81:85	Breast Cancer Cells	67:85	Breast Cancer Cells	67:85	Exerts Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects in LPS-Stimulated RAW 264.7 Cells.
36361674	16	99	theme	treatments	2719:2728	arg1	development					2662:2672	the development	2658:2672	the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes	2658:2773	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	3	100	theme	anticancer	321:330	arg1	drugs					332:336	Many successful anticancer drugs	305:336	Many successful anticancer drugs used in clinical practices	305:363	Many successful anticancer drugs used in clinical practices are derived from natural products.
36361674	10	101	theme	activated	1635:1643	arg1	cells					1647:1651	activated B cells	1635:1651	activated B cells	1635:1651	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	0	102	theme	pinnata	10:16	arg1	Viv					18:20	Santolina pinnata Viv	0:20	Santolina pinnata Viv.	0:21	Santolina pinnata Viv.
36361674	16	103	theme	breast	2734:2739	arg1	cancer					2741:2746	breast cancer	2734:2746	breast cancer	2734:2746	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	4	104	theme	biological	523:532	arg1	properties					534:543	their biological properties	517:543	their biological properties	517:543	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	7	105	theme	cancer	1136:1141	arg1	cells					1143:1147	breast cancer cells	1129:1147	breast cancer cells	1129:1147	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	15	106	theme	pinnata	2467:2473	arg1	potential					2451:2459	the multi-target biological potential	2423:2459	the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects	2423:2525	Herein, the multi-target biological potential of S. pinnata in inducing antitumor and anti-inflammatory effects was comprehensively demonstrated.
36361674	6	107	theme	liquid	1019:1024	arg1	UHPLC					1042:1046	UHPLC	1042:1046	UHPLC	1042:1046	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	6	107	theme	liquid	1019:1024	arg1	chromatography					1026:1039	ultra-high-performance liquid chromatography	996:1039	ultra-high-performance liquid chromatography (UHPLC)	996:1047	n-Hexane (EHSP) and methanol (EMSP) extracts were investigated by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography (UHPLC), respectively.
36361674	3	108	theme	clinical	346:353	arg1	practices					355:363	clinical practices	346:363	clinical practices	346:363	Many successful anticancer drugs used in clinical practices are derived from natural products.
36361674	2	109	theme	causes	181:186	arg1	causes					181:186	the largest causes	169:186	the largest causes of mortality in the world	169:212	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	2	109	theme	causes	181:186	arg1	one					162:164	one	162:164	one	162:164	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	5	110	theme	interesting	725:735	arg1	source					745:750	an interesting natural source	722:750	an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features	722:832	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	4	111	dep	species	434:440	arg1	distributed					442:452	distributed	442:452	distributed in the Mediterranean area	442:478	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	4	111	dep	species	434:440	arg1	used					484:487	used	484:487	used in traditional medicine for their biological properties	484:543	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	10	112	theme	B	1645:1645	arg1	cells					1647:1651	activated B cells	1635:1651	activated B cells	1635:1651	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	4	113	theme	traditional	492:502	arg1	medicine					504:511	traditional medicine	492:511	traditional medicine for their biological properties	492:543	The genus Santolina is a group of species distributed in the Mediterranean area and used in traditional medicine for their biological properties.
36361674	10	114	theme	cells	1647:1651	arg1	kappa-light-chain-enhancer					1605:1630	the nuclear factor kappa-light-chain-enhancer	1586:1630	the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)	1586:1659	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	10	114	theme	cells	1647:1651	arg1	production					1571:1580	nitric oxide (NO) production	1553:1580	nitric oxide (NO) production	1553:1580	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	10	114	theme	cells	1647:1651	arg1	NF-κB					1654:1658	NF-κB	1654:1658	NF-κB	1654:1658	The anti-inflammatory potential was analyzed by nitric oxide (NO) production and the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) staining experiments in bacterial lipopolysaccharides (LPS) which stimulated RAW 264.7 cells.
36361674	7	115	theme	bromide	1259:1265	arg1	assay					1273:1277	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay	1198:1277	the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay	1198:1277	Anti-proliferative activity was analyzed on MCF-7 and MDA-MB-231 breast cancer cells, as well as on non-tumorigenic MCF-10A cells, by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
36361674	14	116	theme	different	2382:2390	arg1	compositions					2401:2412	their different chemical compositions	2376:2412	their different chemical compositions	2376:2412	The different biological behaviours found between the extracts could be related to their different chemical compositions.
36361674	11	117	theme	apoptotic	1847:1855	arg1	death					1857:1861	apoptotic death	1847:1861	apoptotic death	1847:1861	EHSP and EMSP extracts exhibited anticancer activity against breast cancer cells, promoting apoptotic death, as well as decreasing cell migration and invasive behaviours.
36361674	9	118	theme	invasive	1343:1350	arg1	features					1352:1359	invasive features	1343:1359	invasive features	1343:1359	Cell motility and invasive features were examined in highly invasive MDA-MB-231 by wound-healing scratches, while, in both breast cancer cell lines, by gel-zymography experiments.
36361674	2	119	theme	mortality	191:199	arg1	causes					181:186	the largest causes	169:186	the largest causes of mortality in the world	169:212	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	2	120	theme	great	287:291	arg1	importance					293:302	great importance	287:302	great importance	287:302	Cancer is one of the largest causes of mortality in the world, and due to its incidence, the discovery of novel anticancer drugs is of great importance.
36361674	13	121	theme	LPS-stimulated	2261:2274	arg1	cells					2286:2290	LPS-stimulated RAW 264.7 cells	2261:2290	LPS-stimulated RAW 264.7 cells	2261:2290	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	1	122	theme	Antitumor	40:48	arg1	Activity					50:57	Promising Antitumor Activity	30:57	Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects	30:115	Exerts Promising Antitumor Activity against Breast Cancer Cells and Anti-Inflammatory Effects in LPS-Stimulated RAW 264.7 Cells.
36361674	16	123	theme	effective	2684:2692	arg1	treatments					2719:2728	highly effective S. pinnata extract-based treatments	2677:2728	highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes	2677:2773	These findings will provide important stepping-stones for further investigations and may lead to the development of highly effective S. pinnata extract-based treatments for breast cancer and inflammatory processes.
36361674	13	124	theme	inhibitory	2208:2217	arg1	effects					2219:2225	inhibitory effects	2208:2225	inhibitory effects	2208:2225	Both extracts were also able to promote anti-inflammatory effects (IC50 values ranging from 27.5 to 61.14 μg/mL), as well as to reduce NO levels by inducing inhibitory effects on NF-κB nuclear translocation in LPS-stimulated RAW 264.7 cells.
36361674	5	125	theme	valuable	755:762	arg1	phytochemicals					764:777	valuable phytochemicals	755:777	valuable phytochemicals endowed with anticancer and anti-inflammatory features	755:832	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36361674	5	126	from	potential	635:643	arg1	relation					677:684	relation	677:684	relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed	677:850	The aim of this work was to investigate, for the first time, the multi-target biological potential of Italian Santolina pinnata in relation to their chemical profile, by which an interesting natural source of valuable phytochemicals endowed with anticancer and anti-inflammatory features could be assessed.
36479299	12	0	theme	environmentally	1412:1426	arg1	approach					1437:1444	an environmentally friendly approach	1409:1444	an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells	1409:1510	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	7	1	dep	monocultures	611:622	arg1	monocultures					611:622	monocultures	611:622	monocultures (PsL-WSPs and AP-WSPs)	611:645	LYM-1 and A. pullulans 2012 (PsL/AP-WSPs) compared to monocultures (PsL-WSPs and AP-WSPs).
36479299	7	1	dep	monocultures	611:622	arg1	AP-WSPs					638:644	AP-WSPs	638:644	AP-WSPs	638:644	LYM-1 and A. pullulans 2012 (PsL/AP-WSPs) compared to monocultures (PsL-WSPs and AP-WSPs).
36479299	7	1	dep	monocultures	611:622	arg1	PsL-WSPs					625:632	PsL-WSPs	625:632	PsL-WSPs	625:632	LYM-1 and A. pullulans 2012 (PsL/AP-WSPs) compared to monocultures (PsL-WSPs and AP-WSPs).
36479299	0	2	theme	straw	95:99	arg1	fermentation					79:90	co-culture fermentation	68:90	co-culture fermentation of straw and shrimp shell	68:116	Antioxidant properties of water-soluble polysaccharides prepared by co-culture fermentation of straw and shrimp shell.
36479299	8	3	theme	WSP	712:714	arg1	fractions					716:724	three WSP fractions	706:724	three WSP fractions	706:724	FTIR was used to examine the polysaccharide properties of three WSP fractions.
36479299	10	4	theme	PsL/AP-WSPs	1075:1085	arg1	potential					1062:1070	the application potential	1046:1070	the application potential of PsL/AP-WSPs from straw and shrimp shells	1046:1114	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	1	5	theme	water-soluble	161:173	arg1	WSPs					192:195	WSPs	192:195	WSPs	192:195	Herein, we present a method for producing water-soluble polysaccharides (WSPs) by co-culture fermentation of straw and shrimp shells.
36479299	1	5	theme	water-soluble	161:173	arg1	polysaccharides					175:189	water-soluble polysaccharides	161:189	water-soluble polysaccharides (WSPs)	161:196	Herein, we present a method for producing water-soluble polysaccharides (WSPs) by co-culture fermentation of straw and shrimp shells.
36479299	12	6	dep	wastes	1481:1486	arg1	straw					1488:1492	straw	1488:1492	straw	1488:1492	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	12	6	dep	wastes	1481:1486	arg1	shells					1505:1510	shrimp shells	1498:1510	shrimp shells	1498:1510	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	12	7	theme	WSPs	1450:1453	arg1	production					1455:1464	WSPs production	1450:1464	WSPs production from agro-food wastes straw and shrimp shells	1450:1510	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	6	8	theme	WSPs	475:478	arg1	concentrations					480:493	WSPs concentrations	475:493	WSPs concentrations	475:493	WSPs concentrations were higher in co-culture fermentations of Photobacterium sp.
36479299	10	9	theme	better	969:974	arg1	effects					987:993	better scavenging effects	969:993	better scavenging effects on DPPH, ABTS, and OH free radicals	969:1029	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	10	10	theme	scavenging	976:985	arg1	effects					987:993	better scavenging effects	969:993	better scavenging effects on DPPH, ABTS, and OH free radicals	969:1029	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	0	11	theme	shrimp	105:110	arg1	fermentation					79:90	co-culture fermentation	68:90	co-culture fermentation of straw and shrimp shell	68:116	Antioxidant properties of water-soluble polysaccharides prepared by co-culture fermentation of straw and shrimp shell.
36479299	10	12	theme	shrimp	1102:1107	arg1	shells					1109:1114	shrimp shells	1102:1114	shrimp shells	1102:1114	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	9	13	with	glucosamine	827:837	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	13	with	glucosamine	827:837	arg1	ratios					915:920	molar ratios	909:920	molar ratios	909:920	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	8	14	theme	fractions	716:724	arg1	properties					692:701	the polysaccharide properties	673:701	the polysaccharide properties of three WSP fractions	673:724	FTIR was used to examine the polysaccharide properties of three WSP fractions.
36479299	11	15	theme	days	1193:1196	arg1	temperature					1199:1209	temperature	1199:1209	temperature of 31°C	1199:1217	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	15	theme	days	1193:1196	arg1	time					1183:1186	fermentation time	1170:1186	fermentation time of 6 days	1170:1196	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	15	theme	days	1193:1196	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	15	theme	days	1193:1196	arg1	composition					1270:1280	inoculum composition	1261:1280	inoculum composition	1261:1280	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	15	theme	days	1193:1196	arg1	concentration					1229:1241	inoculum concentration	1220:1241	inoculum concentration of 10% (w/v)	1220:1254	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	9	16	with	mannose	810:816	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	16	with	mannose	810:816	arg1	ratios					915:920	molar ratios	909:920	molar ratios	909:920	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	17	with	galactose	849:857	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	17	with	galactose	849:857	arg1	ratios					915:920	molar ratios	909:920	molar ratios	909:920	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	11	18	theme	±	1299:1299	arg1	yield					1129:1133	The maximum yield	1117:1133	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1117:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	18	theme	±	1299:1299	arg1	mg/mL					1306:1310	5.88 ± 0.40 mg/mL	1294:1310	5.88 ± 0.40 mg/mL	1294:1310	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	9	19	theme	WSPs	768:771	arg1	fractions					773:781	three WSPs fractions	762:781	three WSPs fractions	762:781	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	20	theme	molar	909:913	arg1	ratios					915:920	molar ratios	909:920	molar ratios	909:920	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	10	21	from	straw	1092:1096	arg1	potential					1062:1070	the application potential	1046:1070	the application potential of PsL/AP-WSPs from straw and shrimp shells	1046:1114	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	9	22	theme	fractions	773:781	arg1	compositions					746:757	The monosaccharide compositions	727:757	The monosaccharide compositions of three WSPs fractions	727:781	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	23	theme	varying	879:885	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	4	24	theme	Photobacterium	383:396	arg1	sp					398:399	Photobacterium sp	383:399	Photobacterium sp.	383:400	Photobacterium sp.
36479299	2	25	theme	non-pathogen	330:341	arg1	sp					358:359	the non-pathogen Photobacterium sp	326:359	the non-pathogen Photobacterium sp	326:359	The chitin-degrading strain was isolated and genotypically identified as the non-pathogen Photobacterium sp.
36479299	2	25	theme	non-pathogen	330:341	arg1	strain					274:279	The chitin-degrading strain	253:279	The chitin-degrading strain	253:279	The chitin-degrading strain was isolated and genotypically identified as the non-pathogen Photobacterium sp.
36479299	11	26	dep	conditions	1158:1167	arg1	temperature					1199:1209	temperature	1199:1209	temperature of 31°C	1199:1217	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	26	dep	conditions	1158:1167	arg1	time					1183:1186	fermentation time	1170:1186	fermentation time of 6 days	1170:1196	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	26	dep	conditions	1158:1167	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	26	dep	conditions	1158:1167	arg1	composition					1270:1280	inoculum composition	1261:1280	inoculum composition	1261:1280	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	26	dep	conditions	1158:1167	arg1	concentration					1229:1241	inoculum concentration	1220:1241	inoculum concentration of 10% (w/v)	1220:1254	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	0	27	theme	Antioxidant	0:10	arg1	properties					12:21	Antioxidant properties	0:21	Antioxidant properties of water-soluble polysaccharides	0:54	Antioxidant properties of water-soluble polysaccharides prepared by co-culture fermentation of straw and shrimp shell.
36479299	9	28	theme	molecular	887:895	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	0	29	dep	straw	95:99	arg1	shell					112:116	shell	112:116	shell	112:116	Antioxidant properties of water-soluble polysaccharides prepared by co-culture fermentation of straw and shrimp shell.
36479299	11	30	theme	2:1	1285:1287	arg1	temperature					1199:1209	temperature	1199:1209	temperature of 31°C	1199:1217	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	30	theme	2:1	1285:1287	arg1	time					1183:1186	fermentation time	1170:1186	fermentation time of 6 days	1170:1196	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	30	theme	2:1	1285:1287	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	30	theme	2:1	1285:1287	arg1	composition					1270:1280	inoculum composition	1261:1280	inoculum composition	1261:1280	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	30	theme	2:1	1285:1287	arg1	concentration					1229:1241	inoculum concentration	1220:1241	inoculum concentration of 10% (w/v)	1220:1254	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	0	31	theme	water-soluble	26:38	arg1	polysaccharides					40:54	water-soluble polysaccharides	26:54	water-soluble polysaccharides	26:54	Antioxidant properties of water-soluble polysaccharides prepared by co-culture fermentation of straw and shrimp shell.
36479299	8	32	used	used	657:660	arg2	FTIR					648:651	FTIR	648:651	FTIR	648:651	FTIR was used to examine the polysaccharide properties of three WSP fractions.
36479299	11	33	theme	%	1248:1248	arg1	temperature					1199:1209	temperature	1199:1209	temperature of 31°C	1199:1217	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	33	theme	%	1248:1248	arg1	time					1183:1186	fermentation time	1170:1186	fermentation time of 6 days	1170:1196	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	33	theme	%	1248:1248	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	33	theme	%	1248:1248	arg1	composition					1270:1280	inoculum composition	1261:1280	inoculum composition	1261:1280	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	33	theme	%	1248:1248	arg1	concentration					1229:1241	inoculum concentration	1220:1241	inoculum concentration of 10% (w/v)	1220:1254	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	34	theme	31°C	1214:1217	arg1	temperature					1199:1209	temperature	1199:1209	temperature of 31°C	1199:1217	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	34	theme	31°C	1214:1217	arg1	time					1183:1186	fermentation time	1170:1186	fermentation time of 6 days	1170:1196	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	34	theme	31°C	1214:1217	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	34	theme	31°C	1214:1217	arg1	composition					1270:1280	inoculum composition	1261:1280	inoculum composition	1261:1280	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	34	theme	31°C	1214:1217	arg1	concentration					1229:1241	inoculum concentration	1220:1241	inoculum concentration of 10% (w/v)	1220:1254	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	35	theme	fermentation	1170:1181	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	35	theme	fermentation	1170:1181	arg1	time					1183:1186	fermentation time	1170:1186	fermentation time of 6 days	1170:1196	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	10	36	from	shells	1109:1114	arg1	potential					1062:1070	the application potential	1046:1070	the application potential of PsL/AP-WSPs from straw and shrimp shells	1046:1114	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	6	37	theme	sp	553:554	arg1	fermentations					521:533	co-culture fermentations	510:533	co-culture fermentations of Photobacterium sp	510:554	WSPs concentrations were higher in co-culture fermentations of Photobacterium sp.
36479299	12	38	theme	shrimp	1498:1503	arg1	shells					1505:1510	shrimp shells	1498:1510	shrimp shells	1498:1510	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	9	39	theme	monosaccharide	731:744	arg1	compositions					746:757	The monosaccharide compositions	727:757	The monosaccharide compositions of three WSPs fractions	727:781	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	1	40	theme	co-culture	201:210	arg1	fermentation					212:223	co-culture fermentation	201:223	co-culture fermentation of straw and shrimp shells	201:250	Herein, we present a method for producing water-soluble polysaccharides (WSPs) by co-culture fermentation of straw and shrimp shells.
36479299	11	41	theme	PsL/AP-WSPs	1326:1336	arg1	optimization					1349:1360	the PsL/AP-WSPs production optimization	1322:1360	the PsL/AP-WSPs production optimization by orthogonal design	1322:1381	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	6	42	theme	Photobacterium	538:551	arg1	sp					553:554	Photobacterium sp	538:554	Photobacterium sp	538:554	WSPs concentrations were higher in co-culture fermentations of Photobacterium sp.
36479299	10	43	theme	application	1050:1060	arg1	potential					1062:1070	the application potential	1046:1070	the application potential of PsL/AP-WSPs from straw and shrimp shells	1046:1114	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	0	44	theme	polysaccharides	40:54	arg1	properties					12:21	Antioxidant properties	0:21	Antioxidant properties of water-soluble polysaccharides	0:54	Antioxidant properties of water-soluble polysaccharides prepared by co-culture fermentation of straw and shrimp shell.
36479299	11	45	theme	production	1338:1347	arg1	optimization					1349:1360	the PsL/AP-WSPs production optimization	1322:1360	the PsL/AP-WSPs production optimization by orthogonal design	1322:1381	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	1	46	dep	straw	228:232	arg1	shells					245:250	shells	245:250	shells	245:250	Herein, we present a method for producing water-soluble polysaccharides (WSPs) by co-culture fermentation of straw and shrimp shells.
36479299	1	47	theme	shrimp	238:243	arg1	fermentation					212:223	co-culture fermentation	201:223	co-culture fermentation of straw and shrimp shells	201:250	Herein, we present a method for producing water-soluble polysaccharides (WSPs) by co-culture fermentation of straw and shrimp shells.
36479299	11	48	theme	maximum	1121:1127	arg1	yield					1129:1133	The maximum yield	1117:1133	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1117:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	48	theme	maximum	1121:1127	arg1	mg/mL					1306:1310	5.88 ± 0.40 mg/mL	1294:1310	5.88 ± 0.40 mg/mL	1294:1310	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	0	49	theme	co-culture	68:77	arg1	fermentation					79:90	co-culture fermentation	68:90	co-culture fermentation of straw and shrimp shell	68:116	Antioxidant properties of water-soluble polysaccharides prepared by co-culture fermentation of straw and shrimp shell.
36479299	12	50	from	wastes	1481:1486	arg1	production					1455:1464	WSPs production	1450:1464	WSPs production from agro-food wastes straw and shrimp shells	1450:1510	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	11	51	theme	inoculum	1220:1227	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	51	theme	inoculum	1220:1227	arg1	concentration					1229:1241	inoculum concentration	1220:1241	inoculum concentration of 10% (w/v)	1220:1254	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	12	52	theme	friendly	1428:1435	arg1	approach					1437:1444	an environmentally friendly approach	1409:1444	an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells	1409:1510	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	6	53	theme	co-culture	510:519	arg1	fermentations					521:533	co-culture fermentations	510:533	co-culture fermentations of Photobacterium sp	510:554	WSPs concentrations were higher in co-culture fermentations of Photobacterium sp.
36479299	12	54	theme	agro-food	1471:1479	arg1	wastes					1481:1486	agro-food wastes straw and shrimp shells	1471:1510	agro-food wastes straw and shrimp shells	1471:1510	The results suggest that an environmentally friendly approach for WSPs production from agro-food wastes straw and shrimp shells was developed.
36479299	11	55	theme	optimum	1150:1156	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	55	theme	optimum	1150:1156	arg1	time					1183:1186	fermentation time	1170:1186	fermentation time of 6 days	1170:1196	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	55	theme	optimum	1150:1156	arg1	temperature					1199:1209	temperature	1199:1209	temperature of 31°C	1199:1217	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	55	theme	optimum	1150:1156	arg1	composition					1270:1280	inoculum composition	1261:1280	inoculum composition	1261:1280	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	55	theme	optimum	1150:1156	arg1	concentration					1229:1241	inoculum concentration	1220:1241	inoculum concentration of 10% (w/v)	1220:1254	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	56	theme	inoculum	1261:1268	arg1	conditions					1158:1167	optimum conditions	1150:1167	optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1)	1150:1288	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	11	56	theme	inoculum	1261:1268	arg1	composition					1270:1280	inoculum composition	1261:1280	inoculum composition	1261:1280	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	9	57	theme	HPLC	935:938	arg1	analysis					940:947	HPLC analysis	935:947	HPLC analysis	935:947	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	58	with	arabinose	864:872	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	58	with	arabinose	864:872	arg1	ratios					915:920	molar ratios	909:920	molar ratios	909:920	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	1	59	theme	straw	228:232	arg1	fermentation					212:223	co-culture fermentation	201:223	co-culture fermentation of straw and shrimp shells	201:250	Herein, we present a method for producing water-soluble polysaccharides (WSPs) by co-culture fermentation of straw and shrimp shells.
36479299	8	60	theme	polysaccharide	677:690	arg1	properties					692:701	the polysaccharide properties	673:701	the polysaccharide properties of three WSP fractions	673:724	FTIR was used to examine the polysaccharide properties of three WSP fractions.
36479299	2	61	theme	chitin-degrading	257:272	arg1	sp					358:359	the non-pathogen Photobacterium sp	326:359	the non-pathogen Photobacterium sp	326:359	The chitin-degrading strain was isolated and genotypically identified as the non-pathogen Photobacterium sp.
36479299	2	61	theme	chitin-degrading	257:272	arg1	strain					274:279	The chitin-degrading strain	253:279	The chitin-degrading strain	253:279	The chitin-degrading strain was isolated and genotypically identified as the non-pathogen Photobacterium sp.
36479299	2	62	theme	Photobacterium	343:356	arg1	sp					358:359	the non-pathogen Photobacterium sp	326:359	the non-pathogen Photobacterium sp	326:359	The chitin-degrading strain was isolated and genotypically identified as the non-pathogen Photobacterium sp.
36479299	2	62	theme	Photobacterium	343:356	arg1	strain					274:279	The chitin-degrading strain	253:279	The chitin-degrading strain	253:279	The chitin-degrading strain was isolated and genotypically identified as the non-pathogen Photobacterium sp.
36479299	5	63	theme	Aureobasidium	412:424	arg1	2012					436:439	Aureobasidium pullulans 2012	412:439	Aureobasidium pullulans 2012	412:439	LYM-1 and Aureobasidium pullulans 2012 could coexist without antagonism.
36479299	11	64	theme	orthogonal	1365:1374	arg1	design					1376:1381	orthogonal design	1365:1381	orthogonal design	1365:1381	The maximum yield obtained under optimum conditions (fermentation time of 6 days, temperature of 31°C, inoculum concentration of 10% (w/v), and inoculum composition of 2:1) was 5.88 ± 0.40 mg/mL, based on the PsL/AP-WSPs production optimization by orthogonal design.
36479299	3	65	from	LYM-1	362:366	arg1	study					376:380	this study	371:380	this study	371:380	LYM-1 in this study.
36479299	10	66	theme	free	1017:1020	arg1	radicals					1022:1029	OH free radicals	1014:1029	OH free radicals	1014:1029	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	5	67	theme	pullulans	426:434	arg1	2012					436:439	Aureobasidium pullulans 2012	412:439	Aureobasidium pullulans 2012	412:439	LYM-1 and Aureobasidium pullulans 2012 could coexist without antagonism.
36479299	9	68	with	glucose	840:846	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	68	with	glucose	840:846	arg1	ratios					915:920	molar ratios	909:920	molar ratios	909:920	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	10	69	theme	OH	1014:1015	arg1	radicals					1022:1029	OH free radicals	1014:1029	OH free radicals	1014:1029	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	10	70	from	effects	987:993	arg1	radicals					1022:1029	OH free radicals	1014:1029	OH free radicals	1014:1029	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	10	70	from	effects	987:993	arg1	ABTS					1004:1007	ABTS	1004:1007	ABTS	1004:1007	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	10	70	from	effects	987:993	arg1	DPPH					998:1001	DPPH	998:1001	DPPH	998:1001	PsL/AP-WSPs showed better scavenging effects on DPPH, ABTS, and OH free radicals, demonstrating the application potential of PsL/AP-WSPs from straw and shrimp shells.
36479299	9	71	with	ribose	819:824	arg1	weights					897:903	varying molecular weights	879:903	varying molecular weights	879:903	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36479299	9	71	with	ribose	819:824	arg1	ratios					915:920	molar ratios	909:920	molar ratios	909:920	The monosaccharide compositions of three WSPs fractions were primarily composed of mannose, ribose, glucosamine, glucose, galactose, and arabinose with varying molecular weights and molar ratios according to HPLC analysis.
36943475	8	0	theme	potatoes	1708:1715	arg1	diversity					1689:1697	the genetic diversity	1677:1697	the genetic diversity of sweet potatoes	1677:1715	This results indicated that sugar and starch are important characteristics to determine the genetic diversity of sweet potatoes.
36943475	3	1	theme	different	452:460	arg1	localities					462:471	different localities	452:471	different localities	452:471	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	3	1	theme	different	452:460	arg1	cultivars					526:534	5 local cultivars	518:534	5 local cultivars	518:534	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	3	1	theme	different	452:460	arg1	cultivars					507:515	36 modern cultivars	497:515	36 modern cultivars	497:515	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	3	1	theme	different	452:460	arg1	cultivars					552:560	25 foreign cultivars	541:560	25 foreign cultivars	541:560	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	7	2	theme	galactose	1326:1334	arg1	metabolism					1336:1345	galactose metabolism	1326:1345	galactose metabolism (itf04g30630)	1326:1359	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	2	theme	galactose	1326:1334	arg1	itf04g30630					1348:1358	itf04g30630	1348:1358	itf04g30630	1348:1358	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	8	3	theme	sweet	1702:1706	arg1	potatoes					1708:1715	sweet potatoes	1702:1715	sweet potatoes	1702:1715	This results indicated that sugar and starch are important characteristics to determine the genetic diversity of sweet potatoes.
36943475	6	4	theme	storage	1140:1146	arg1	root					1148:1151	the storage root	1136:1151	the storage root	1136:1151	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	7	5	theme	sugar	1544:1548	arg1	metabolism					1550:1559	amino sugar and nucleotide sugar metabolism	1517:1559	metabolism	1550:1559	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	6	6	theme	genome-wide	981:991	arg1	association					993:1003	genome-wide association	981:1003	genome-wide association	981:1003	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	7	theme	root	1148:1151	arg1	starch					1087:1092	total starch	1081:1092	total starch	1081:1092	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	7	theme	root	1148:1151	arg1	content					1103:1109	amylose content	1095:1109	amylose content	1095:1109	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	7	theme	root	1148:1151	arg1	traits					1035:1040	sugar composition-related traits	1009:1040	sugar composition-related traits (fructose, glucose, and total sugars)	1009:1078	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	7	theme	root	1148:1151	arg1	carotenoid					1122:1131	total carotenoid	1116:1131	total carotenoid of the storage root	1116:1151	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	8	8	theme	important	1638:1646	arg1	starch					1627:1632	starch	1627:1632	starch	1627:1632	This results indicated that sugar and starch are important characteristics to determine the genetic diversity of sweet potatoes.
36943475	8	8	theme	important	1638:1646	arg1	characteristics					1648:1662	important characteristics	1638:1662	important characteristics	1638:1662	This results indicated that sugar and starch are important characteristics to determine the genetic diversity of sweet potatoes.
36943475	8	8	theme	important	1638:1646	arg1	sugar					1617:1621	sugar	1617:1621	sugar	1617:1621	This results indicated that sugar and starch are important characteristics to determine the genetic diversity of sweet potatoes.
36943475	7	9	theme	candidate	1168:1176	arg1	genes					1178:1182	37 candidate genes	1165:1182	37 candidate genes encompassing these significant SNPs	1165:1218	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	10	dep	sugar	1523:1527	arg1	itf15g04880					1575:1585	itf15g04880	1575:1585	itf15g04880	1575:1585	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	0	11	theme	component	102:110	arg1	traits					112:117	component traits	102:117	component traits in sweet potato (Ipomoea batatas (L.) Lam)	102:160	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	3	12	theme	modern	500:505	arg1	cultivars					507:515	36 modern cultivars	497:515	36 modern cultivars	497:515	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	2	13	theme	genetic	314:320	arg1	diversity					322:330	their genetic diversity	308:330	their genetic diversity	308:330	This issue enriched their genetic diversity but also resulted in a mixture of cultivars.
36943475	4	14	theme	nucleotide	666:675	arg1	SNPs					692:695	SNPs	692:695	SNPs	692:695	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	4	14	theme	nucleotide	666:675	arg1	polymorphisms					677:689	39,424 single nucleotide polymorphisms	652:689	39,424 single nucleotide polymorphisms (SNPs)	652:696	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	0	15	theme	sweet	122:126	arg1	potato					128:133	sweet potato	122:133	sweet potato (Ipomoea batatas (L.) Lam)	122:160	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	0	15	theme	sweet	122:126	arg1	Lam					157:159	Ipomoea batatas (L.) Lam	136:159	Ipomoea batatas (L.) Lam	136:159	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	5	16	theme	genetic	811:817	arg1	group					854:858	group 4	854:860	group 4	854:860	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	16	theme	genetic	811:817	arg1	groups					819:824	5 genetic groups	809:824	5 genetic groups	809:824	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	16	theme	genetic	811:817	arg1	group					845:849	group 3	845:851	group 3	845:851	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	16	theme	genetic	811:817	arg1	group					867:871	group 5	867:873	group 5	867:873	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	16	theme	genetic	811:817	arg1	group					827:831	group 1	827:833	group 1	827:833	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	16	theme	genetic	811:817	arg1	group					836:840	group 2	836:842	group 2	836:842	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	6	17	theme	total	1066:1070	arg1	sugars					1072:1077	total sugars	1066:1077	total sugars	1066:1077	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	7	18	theme	nucleotide	1533:1542	arg1	metabolism					1550:1559	amino sugar and nucleotide sugar metabolism	1517:1559	metabolism	1550:1559	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	5	19	theme	structure	746:754	arg1	analysis					756:763	population structure analysis	735:763	population structure analysis	735:763	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	7	20	theme	starch	1297:1302	arg1	metabolism					1304:1313	starch metabolism	1297:1313	starch metabolism	1297:1313	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	5	21	theme	population	735:744	arg1	analysis					756:763	population structure analysis	735:763	population structure analysis	735:763	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	8	22	theme	genetic	1681:1687	arg1	diversity					1689:1697	the genetic diversity	1677:1697	the genetic diversity of sweet potatoes	1677:1715	This results indicated that sugar and starch are important characteristics to determine the genetic diversity of sweet potatoes.
36943475	4	23	dep	mapping	625:631	arg1	reads					633:637	reads	633:637	reads	633:637	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	7	24	theme	amino	1517:1521	arg1	sugar					1523:1527	amino sugar and nucleotide sugar metabolism	1517:1559	sugar	1523:1527	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	25	theme	significant	1203:1213	arg1	SNPs					1215:1218	these significant SNPs	1197:1218	these significant SNPs	1197:1218	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	0	26	theme	diversity	8:16	arg1	assessment					18:27	Genetic diversity assessment	0:27	Genetic diversity assessment	0:27	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	9	27	theme	genetic	1867:1873	arg1	diversity					1875:1883	genetic diversity	1867:1883	genetic diversity of sweet potato	1867:1899	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
36943475	9	28	theme	potato	1894:1899	arg1	diversity					1875:1883	genetic diversity	1867:1883	genetic diversity of sweet potato	1867:1899	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
36943475	5	29	contain	containing	876:885	arg2	accessions					909:918	20, 15, 10, 7, and 14 accessions	887:918	20, 15, 10, 7, and 14 accessions	887:918	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	29	contain	containing	876:885	arg1	group					854:858	group 4	854:860	group 4	854:860	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	29	contain	containing	876:885	arg1	groups					819:824	5 genetic groups	809:824	5 genetic groups	809:824	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	29	contain	containing	876:885	arg1	group					845:849	group 3	845:851	group 3	845:851	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	29	contain	containing	876:885	arg1	group					867:871	group 5	867:873	group 5	867:873	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	29	contain	containing	876:885	arg1	group					827:831	group 1	827:833	group 1	827:833	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	5	29	contain	containing	876:885	arg1	group					836:840	group 2	836:842	group 2	836:842	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	4	30	theme	million	617:623	arg1	mapping					625:631	324.8 million mapping	611:631	324.8 million mapping reads	611:637	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	0	31	theme	Genetic	0:6	arg1	diversity					8:16	Genetic diversity	0:16	Genetic diversity assessment	0:27	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	6	32	theme	total	1116:1120	arg1	traits					1035:1040	sugar composition-related traits	1009:1040	sugar composition-related traits (fructose, glucose, and total sugars)	1009:1078	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	32	theme	total	1116:1120	arg1	carotenoid					1122:1131	total carotenoid	1116:1131	total carotenoid of the storage root	1116:1151	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	3	33	theme	local	520:524	arg1	cultivars					526:534	5 local cultivars	518:534	5 local cultivars	518:534	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	9	34	theme	traits	1781:1786	arg1	importance					1757:1766	the importance	1753:1766	the importance of component traits to genotyping sweet potatoes	1753:1815	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
36943475	1	35	theme	Korean	167:172	arg1	potatoes					180:187	The Korean sweet potatoes	163:187	The Korean sweet potatoes	163:187	The Korean sweet potatoes were bred by various cultivars introduced from Japanese, American, Porto Rico, China, and Burundi.
36943475	6	36	theme	total	1081:1085	arg1	starch					1087:1092	total starch	1081:1092	total starch	1081:1092	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	36	theme	total	1081:1085	arg1	traits					1035:1040	sugar composition-related traits	1009:1040	sugar composition-related traits (fructose, glucose, and total sugars)	1009:1078	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	1	37	theme	sweet	174:178	arg1	potatoes					180:187	The Korean sweet potatoes	163:187	The Korean sweet potatoes	163:187	The Korean sweet potatoes were bred by various cultivars introduced from Japanese, American, Porto Rico, China, and Burundi.
36943475	6	38	theme	sugar	1009:1013	arg1	starch					1087:1092	total starch	1081:1092	total starch	1081:1092	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	38	theme	sugar	1009:1013	arg1	traits					1035:1040	sugar composition-related traits	1009:1040	sugar composition-related traits (fructose, glucose, and total sugars)	1009:1078	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	38	theme	sugar	1009:1013	arg1	carotenoid					1122:1131	total carotenoid	1116:1131	total carotenoid of the storage root	1116:1151	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	38	theme	sugar	1009:1013	arg1	content					1103:1109	amylose content	1095:1109	amylose content	1095:1109	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	38	theme	sugar	1009:1013	arg1	fructose					1043:1050	fructose	1043:1050	fructose	1043:1050	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	38	theme	sugar	1009:1013	arg1	glucose					1053:1059	glucose	1053:1059	glucose	1053:1059	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	7	39	theme	amino	1476:1480	arg1	itf12g19270					1499:1509	itf12g19270	1499:1509	itf12g19270	1499:1509	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	39	theme	amino	1476:1480	arg1	metabolism					1487:1496	carbohydrate and amino acid metabolism	1459:1496	metabolism	1487:1496	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	40	theme	genes	1178:1182	arg1	total					1156:1160	A total	1154:1160	A total of 37 candidate genes encompassing these significant SNPs	1154:1218	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	9	41	theme	genotyping	1791:1800	arg1	potatoes					1808:1815	genotyping sweet potatoes	1791:1815	genotyping sweet potatoes	1791:1815	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
36943475	2	42	theme	cultivars	366:374	arg1	mixture					355:361	a mixture	353:361	a mixture of cultivars	353:374	This issue enriched their genetic diversity but also resulted in a mixture of cultivars.
36943475	0	43	theme	association	45:55	arg1	study					57:61	genome-wide association study	33:61	genome-wide association study	33:61	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	7	44	theme	acid	1482:1485	arg1	itf12g19270					1499:1509	itf12g19270	1499:1509	itf12g19270	1499:1509	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	44	theme	acid	1482:1485	arg1	metabolism					1487:1496	carbohydrate and amino acid metabolism	1459:1496	metabolism	1487:1496	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	0	45	theme	batatas	144:150	arg1	potato					128:133	sweet potato	122:133	sweet potato (Ipomoea batatas (L.) Lam)	122:160	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	0	45	theme	batatas	144:150	arg1	Lam					157:159	Ipomoea batatas (L.) Lam	136:159	Ipomoea batatas (L.) Lam	136:159	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	4	46	theme	324.8	611:615	arg1	million					617:623	million	617:623	million	617:623	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	4	47	theme	trimmed	593:599	arg1	million					585:591	447.6 million trimmed reads	579:605	447.6 million trimmed reads	579:605	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	0	48	theme	genome-wide	33:43	arg1	association					45:55	genome-wide association	33:55	genome-wide association study	33:61	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	7	49	theme	carbohydrate	1459:1470	arg1	itf12g19270					1499:1509	itf12g19270	1499:1509	itf12g19270	1499:1509	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	49	theme	carbohydrate	1459:1470	arg1	metabolism					1487:1496	carbohydrate and amino acid metabolism	1459:1496	metabolism	1487:1496	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	0	50	theme	Ipomoea	136:142	arg1	potato					128:133	sweet potato	122:133	sweet potato (Ipomoea batatas (L.) Lam)	122:160	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	0	50	theme	Ipomoea	136:142	arg1	Lam					157:159	Ipomoea batatas (L.) Lam	136:159	Ipomoea batatas (L.) Lam	136:159	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	7	51	theme	sucrose	1373:1379	arg1	metabolism					1381:1390	starch and sucrose metabolism	1362:1390	metabolism	1381:1390	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	4	52	dep	million	585:591	arg1	reads					601:605	reads	601:605	reads	601:605	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	0	53	theme	L.	153:154	arg1	potato					128:133	sweet potato	122:133	sweet potato (Ipomoea batatas (L.) Lam)	122:160	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	0	53	theme	L.	153:154	arg1	Lam					157:159	Ipomoea batatas (L.) Lam	136:159	Ipomoea batatas (L.) Lam	136:159	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	5	54	theme	Phylogenetic	707:718	arg1	clustering					720:729	Phylogenetic clustering	707:729	Phylogenetic clustering	707:729	Phylogenetic clustering and population structure analysis distinctly classified these germplasms into 5 genetic groups, group 1, group 2, group 3, group 4, and group 5, containing 20, 15, 10, 7, and 14 accessions, respectively.
36943475	6	55	theme	composition-related	1015:1033	arg1	starch					1087:1092	total starch	1081:1092	total starch	1081:1092	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	55	theme	composition-related	1015:1033	arg1	traits					1035:1040	sugar composition-related traits	1009:1040	sugar composition-related traits (fructose, glucose, and total sugars)	1009:1078	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	55	theme	composition-related	1015:1033	arg1	carotenoid					1122:1131	total carotenoid	1116:1131	total carotenoid of the storage root	1116:1151	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	55	theme	composition-related	1015:1033	arg1	content					1103:1109	amylose content	1095:1109	amylose content	1095:1109	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	55	theme	composition-related	1015:1033	arg1	fructose					1043:1050	fructose	1043:1050	fructose	1043:1050	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	55	theme	composition-related	1015:1033	arg1	glucose					1053:1059	glucose	1053:1059	glucose	1053:1059	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	4	56	theme	single	659:664	arg1	SNPs					692:695	SNPs	692:695	SNPs	692:695	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	4	56	theme	single	659:664	arg1	polymorphisms					677:689	39,424 single nucleotide polymorphisms	652:689	39,424 single nucleotide polymorphisms (SNPs)	652:696	This identified 447.6 million trimmed reads and 324.8 million mapping reads and provided 39,424 single nucleotide polymorphisms (SNPs) markers.
36943475	7	57	theme	starch	1362:1367	arg1	metabolism					1381:1390	starch and sucrose metabolism	1362:1390	metabolism	1381:1390	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	6	58	theme	amylose	1095:1101	arg1	content					1103:1109	amylose content	1095:1109	amylose content	1095:1109	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	58	theme	amylose	1095:1101	arg1	traits					1035:1040	sugar composition-related traits	1009:1040	sugar composition-related traits (fructose, glucose, and total sugars)	1009:1078	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	9	59	theme	sweet	1802:1806	arg1	potatoes					1808:1815	genotyping sweet potatoes	1791:1815	genotyping sweet potatoes	1791:1815	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
36943475	9	60	theme	sweet	1888:1892	arg1	potato					1894:1899	sweet potato	1888:1899	sweet potato	1888:1899	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
36943475	7	61	dep	metabolism	1381:1390	arg1	itf15g09320					1406:1416	itf15g09320	1406:1416	itf15g09320	1406:1416	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	3	62	theme	sweet	423:427	arg1	germplasms					436:445	66 sweet potato germplasms	420:445	66 sweet potato germplasms	420:445	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	0	63	from	traits	112:117	arg1	potato					128:133	sweet potato	122:133	sweet potato (Ipomoea batatas (L.) Lam)	122:160	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	0	63	from	traits	112:117	arg1	Lam					157:159	Ipomoea batatas (L.) Lam	136:159	Ipomoea batatas (L.) Lam	136:159	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	6	64	theme	significant	947:957	arg1	SNPs					959:962	Sixty-three significant SNPs	935:962	Sixty-three significant SNPs	935:962	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	3	65	theme	potato	429:434	arg1	germplasms					436:445	66 sweet potato germplasms	420:445	66 sweet potato germplasms	420:445	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	9	66	theme	component	1771:1779	arg1	traits					1781:1786	component traits	1771:1786	component traits	1771:1786	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
36943475	1	67	theme	various	202:208	arg1	cultivars					210:218	various cultivars	202:218	various cultivars introduced from Japanese	202:243	The Korean sweet potatoes were bred by various cultivars introduced from Japanese, American, Porto Rico, China, and Burundi.
36943475	1	67	theme	various	202:208	arg1	Rico					262:265	Rico	262:265	Rico	262:265	The Korean sweet potatoes were bred by various cultivars introduced from Japanese, American, Porto Rico, China, and Burundi.
36943475	1	67	theme	various	202:208	arg1	China					268:272	China	268:272	China	268:272	The Korean sweet potatoes were bred by various cultivars introduced from Japanese, American, Porto Rico, China, and Burundi.
36943475	1	67	theme	various	202:208	arg1	Burundi					279:285	Burundi	279:285	Burundi	279:285	The Korean sweet potatoes were bred by various cultivars introduced from Japanese, American, Porto Rico, China, and Burundi.
36943475	3	68	theme	foreign	544:550	arg1	cultivars					552:560	25 foreign cultivars	541:560	25 foreign cultivars	541:560	For genotyping, we collected and sequenced 66 sweet potato germplasms from different localities around Korea, including 36 modern cultivars, 5 local cultivars, and 25 foreign cultivars.
36943475	6	69	theme	Sixty-three	935:945	arg1	SNPs					959:962	Sixty-three significant SNPs	935:962	Sixty-three significant SNPs	935:962	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	7	70	theme	carbohydrate	1420:1431	arg1	metabolism					1433:1442	carbohydrate metabolism	1420:1442	carbohydrate metabolism (itf14g10250)	1420:1456	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	7	70	theme	carbohydrate	1420:1431	arg1	itf14g10250					1445:1455	itf14g10250	1445:1455	itf14g10250	1445:1455	A total of 37 candidate genes encompassing these significant SNPs were identified, among which, 7 genes were annotated to involve in sugar and starch metabolism, including galactose metabolism (itf04g30630), starch and sucrose metabolism (itf03g13270, itf15g09320), carbohydrate metabolism (itf14g10250), carbohydrate and amino acid metabolism (itf12g19270), and amino sugar and nucleotide sugar metabolism (itf03g21950, itf15g04880).
36943475	0	71	theme	candidate	70:78	arg1	genes					80:84	candidate genes	70:84	candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam)	70:160	Genetic diversity assessment and genome-wide association study reveal candidate genes associated with component traits in sweet potato (Ipomoea batatas (L.) Lam).
36943475	6	72	dep	traits	1035:1040	arg1	glucose					1053:1059	glucose	1053:1059	glucose	1053:1059	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	72	dep	traits	1035:1040	arg1	traits					1035:1040	sugar composition-related traits	1009:1040	sugar composition-related traits (fructose, glucose, and total sugars)	1009:1078	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	72	dep	traits	1035:1040	arg1	fructose					1043:1050	fructose	1043:1050	fructose	1043:1050	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	6	72	dep	traits	1035:1040	arg1	sugars					1072:1077	total sugars	1066:1077	total sugars	1066:1077	Sixty-three significant SNPs were selected by genome-wide association for sugar composition-related traits (fructose, glucose, and total sugars), total starch, amylose content, and total carotenoid of the storage root.
36943475	9	73	theme	important	1837:1845	arg1	reason					1847:1852	an important reason	1834:1852	an important reason resulting in genetic diversity of sweet potato	1834:1899	These findings not only illustrate the importance of component traits to genotyping sweet potatoes but also explain an important reason resulting in genetic diversity of sweet potato.
35580016	9	0	dep	in	1258:1259	arg1	silico					1261:1266	silico	1261:1266	silico	1261:1266	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	2	1	theme	strain	119:124	arg1	ITR2T					126:130	strain ITR2T	119:130	strain ITR2T	119:130	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	8	2	theme	unidentified	1234:1245	arg1	lipid					1247:1251	one unidentified lipid	1230:1251	one unidentified lipid	1230:1251	Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid.
35580016	12	3	theme	novel	1708:1712	arg1	species					1714:1720	a novel species	1706:1720	a novel species within the genus Tumebacillus	1706:1750	nov. to be a novel species within the genus Tumebacillus.
35580016	12	3	theme	novel	1708:1712	arg1	nov.					1695:1698	nov.	1695:1698	nov.	1695:1698	nov. to be a novel species within the genus Tumebacillus.
35580016	4	4	theme	Tumebacillus	526:537	arg1	GST4T					551:555	Tumebacillus flagellatus GST4T	526:555	Tumebacillus flagellatus GST4T (97.9 %)	526:564	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	4	4	theme	Tumebacillus	526:537	arg1	%					563:563	97.9 %	558:563	97.9 %	558:563	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	11	5	dep	Tumebacillus	1665:1676	arg1	amylolyticus					1678:1689	amylolyticus	1678:1689	amylolyticus	1678:1689	Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp.
35580016	4	6	theme	Eur1	480:483	arg1	9.5T					485:488	Tumebacillus permanentifrigoris Eur1 9.5T	448:488	Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %)	448:497	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	4	6	theme	Eur1	480:483	arg1	%					496:496	98.3 %	491:496	98.3 %	491:496	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	7	7	theme	major	972:976	arg1	phosphatidylmonomethylethanolamine					996:1029	phosphatidylmonomethylethanolamine	996:1029	phosphatidylmonomethylethanolamine	996:1029	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	7	theme	major	972:976	arg1	lipids					984:989	the major polar lipids	968:989	the major polar lipids	968:989	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	4	8	theme	novel	405:409	arg1	strain					411:416	the novel strain	401:416	the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %)	401:564	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	7	9	theme	unidentified	1087:1098	arg1	phospholipid					1100:1111	one unidentified phospholipid	1083:1111	one unidentified phospholipid	1083:1111	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	4	10	theme	permanentifrigoris	461:478	arg1	9.5T					485:488	Tumebacillus permanentifrigoris Eur1 9.5T	448:488	Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %)	448:497	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	4	10	theme	permanentifrigoris	461:478	arg1	%					496:496	98.3 %	491:496	98.3 %	491:496	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	1	11	theme	garden	50:55	arg1	soil					57:60	garden soil	50:60	garden soil in Korea	50:69	nov., isolated from garden soil in Korea.
35580016	1	12	from	soil	57:60	arg1	Korea					65:69	Korea	65:69	Korea	65:69	nov., isolated from garden soil in Korea.
35580016	11	13	theme	present	1639:1645	arg1	study					1647:1651	the present study	1635:1651	the present study	1635:1651	Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp.
35580016	4	14	theme	16S	369:371	arg1	analysis					383:390	16S rRNA gene analysis	369:390	16S rRNA gene analysis	369:390	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	11	15	theme	Tumebacillus	1665:1676	arg1	sp					1691:1692	Tumebacillus amylolyticus sp	1665:1692	Tumebacillus amylolyticus sp	1665:1692	Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp.
35580016	6	16	theme	cell-wall	718:726	arg1	peptidoglycan					728:740	the cell-wall peptidoglycan	714:740	the cell-wall peptidoglycan	714:740	The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan).
35580016	13	17	theme	type	1757:1760	arg1	strain					1762:1767	The type strain	1753:1767	The type strain	1753:1767	The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
35580016	13	17	theme	type	1757:1760	arg1	ITR2T					1772:1776	ITR2T	1772:1776	ITR2T (=KCTC 43280T=NBRC 114753T)	1772:1804	The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
35580016	7	18	dep	C16 	907:910	arg1	 0					925:926	 0	925:926	 0	925:926	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	18	dep	C16 	907:910	arg1	 0					938:939	 0	938:939	 0	938:939	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	18	dep	C16 	907:910	arg1	anteiso-C15 					945:956	anteiso-C15 	945:956	anteiso-C15 	945:956	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	18	dep	C16 	907:910	arg1	 0					958:959	 0	958:959	 0	958:959	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	18	dep	C16 	907:910	arg1	 0					912:913	 0	912:913	 0	912:913	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	9	19	theme	T.	1345:1346	arg1	18773T					1371:1376	T. permanentifrigoris DSM 18773T	1345:1376	T. permanentifrigoris DSM 18773T	1345:1376	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	19	theme	T.	1345:1346	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T)	1322:1402	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	5	20	theme	%	672:672	arg1	concentrations					650:663	NaCl concentrations	645:663	NaCl concentrations of 0-1 %	645:672	Strain ITR2T grew optimally at temperatures of 20-30 °C, at pH 6.0-7.0 and at NaCl concentrations of 0-1 %.
35580016	6	21	theme	meso-diaminopimelic	802:820	arg1	acid					822:825	meso-diaminopimelic acid	802:825	meso-diaminopimelic acid	802:825	The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan).
35580016	7	22	theme	fatty	863:867	arg1	C16 					907:910	C16 	907:910	C16 	907:910	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	22	theme	fatty	863:867	arg1	acids					869:873	The major fatty acids	853:873	The major fatty acids (>10%) of the novel strain	853:900	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	22	theme	fatty	863:867	arg1	%					879:879	>10%	876:879	>10%	876:879	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	9	23	theme	strain	1305:1310	arg1	ITR2T					1312:1316	strain ITR2T	1305:1316	strain ITR2T	1305:1316	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	24	theme	permanentifrigoris	1348:1365	arg1	18773T					1371:1376	T. permanentifrigoris DSM 18773T	1345:1376	T. permanentifrigoris DSM 18773T	1345:1376	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	24	theme	permanentifrigoris	1348:1365	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T)	1322:1402	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	0	25	theme	amylolyticus	13:24	arg1	sp					26:27	Tumebacillus amylolyticus sp	0:27	Tumebacillus amylolyticus sp.	0:28	Tumebacillus amylolyticus sp.
35580016	4	26	theme	separate	428:435	arg1	clade					437:441	a separate clade	426:441	a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %)	426:497	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	9	27	theme	nucleotide	1458:1467	arg1	%					1504:1504	78.5 and 81.2 %	1490:1504	%	1504:1504	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	27	theme	nucleotide	1458:1467	arg1	values					1478:1483	the average nucleotide identity values	1446:1483	the average nucleotide identity values	1446:1483	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	13	28	theme	43280T=NBRC	1785:1795	arg1	114753T					1797:1803	=KCTC 43280T=NBRC 114753T	1779:1803	=KCTC 43280T=NBRC 114753T	1779:1803	The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
35580016	13	28	theme	43280T=NBRC	1785:1795	arg1	ITR2T					1772:1776	ITR2T	1772:1776	ITR2T (=KCTC 43280T=NBRC 114753T)	1772:1804	The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
35580016	8	29	theme	strain	1146:1151	arg1	lipids					1126:1131	Other polar lipids	1114:1131	Other polar lipids of the novel strain	1114:1151	Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid.
35580016	0	30	theme	Tumebacillus	0:11	arg1	sp					26:27	Tumebacillus amylolyticus sp	0:27	Tumebacillus amylolyticus sp.	0:28	Tumebacillus amylolyticus sp.
35580016	9	31	theme	identity	1469:1476	arg1	%					1504:1504	78.5 and 81.2 %	1490:1504	%	1504:1504	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	31	theme	identity	1469:1476	arg1	values					1478:1483	the average nucleotide identity values	1446:1483	the average nucleotide identity values	1446:1483	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	8	32	theme	novel	1140:1144	arg1	strain					1146:1151	the novel strain	1136:1151	the novel strain	1136:1151	Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid.
35580016	12	33	theme	genus	1733:1737	arg1	Tumebacillus					1739:1750	the genus Tumebacillus	1729:1750	the genus Tumebacillus	1729:1750	nov. to be a novel species within the genus Tumebacillus.
35580016	7	34	theme	major	857:861	arg1	C16 					907:910	C16 	907:910	C16 	907:910	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	34	theme	major	857:861	arg1	acids					869:873	The major fatty acids	853:873	The major fatty acids (>10%) of the novel strain	853:900	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	34	theme	major	857:861	arg1	%					879:879	>10%	876:879	>10%	876:879	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	10	35	theme	54.9 mol	1553:1560	arg1	content					1541:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content was 54.9 mol%.
35580016	10	35	theme	54.9 mol	1553:1560	arg1	%					1561:1561	54.9 mol%	1553:1561	54.9 mol%	1553:1561	The genomic DNA G+C content was 54.9 mol%.
35580016	2	36	from	located	213:219	arg1	Republic					232:239	Republic	232:239	Republic	232:239	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	9	37	theme	T.	1382:1383	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T)	1322:1402	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	37	theme	T.	1382:1383	arg1	GST4T					1397:1401	T. flagellatus GST4T	1382:1401	T. flagellatus GST4T	1382:1401	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	38	theme	reference	1326:1334	arg1	18773T					1371:1376	T. permanentifrigoris DSM 18773T	1345:1376	T. permanentifrigoris DSM 18773T	1345:1376	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	38	theme	reference	1326:1334	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T)	1322:1402	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	38	theme	reference	1326:1334	arg1	GST4T					1397:1401	T. flagellatus GST4T	1382:1401	T. flagellatus GST4T	1382:1401	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	2	39	theme	soil	153:156	arg1	sample					158:163	a soil sample	151:163	a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea	151:248	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	9	40	dep	strains	1336:1342	arg1	18773T					1371:1376	T. permanentifrigoris DSM 18773T	1345:1376	T. permanentifrigoris DSM 18773T	1345:1376	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	40	dep	strains	1336:1342	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T)	1322:1402	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	40	dep	strains	1336:1342	arg1	GST4T					1397:1401	T. flagellatus GST4T	1382:1401	T. flagellatus GST4T	1382:1401	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	41	theme	23.9 	1418:1422	arg1	%					1423:1423	21.3 and 23.9 %	1409:1423	%	1423:1423	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	8	42	theme	Other	1114:1118	arg1	lipids					1126:1131	Other polar lipids	1114:1131	Other polar lipids of the novel strain	1114:1151	Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid.
35580016	9	43	theme	in	1258:1259	arg1	hybridization					1276:1288	The in silico DNA-DNA hybridization	1254:1288	The in silico DNA-DNA hybridization values between strain ITR2T	1254:1316	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	8	44	theme	unidentified	1167:1178	arg1	glycolipids					1180:1190	two unidentified glycolipids	1163:1190	two unidentified glycolipids	1163:1190	Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid.
35580016	9	45	theme	78.5	1490:1493	arg1	%					1504:1504	78.5 and 81.2 %	1490:1504	%	1504:1504	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	45	theme	78.5	1490:1493	arg1	values					1478:1483	the average nucleotide identity values	1446:1483	the average nucleotide identity values	1446:1483	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	10	46	theme	genomic	1525:1531	arg1	content					1541:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content was 54.9 mol%.
35580016	10	46	theme	genomic	1525:1531	arg1	%					1561:1561	54.9 mol%	1553:1561	54.9 mol%	1553:1561	The genomic DNA G+C content was 54.9 mol%.
35580016	2	47	attach	isolated	137:144	arg2	strain					97:102	A starch-degrading novel strain	72:102	A starch-degrading novel strain	72:102	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	2	47	attach	isolated	137:144	arg1	sample					158:163	a soil sample	151:163	a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea	151:248	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	2	48	theme	novel	91:95	arg1	strain					97:102	A starch-degrading novel strain	72:102	A starch-degrading novel strain	72:102	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	5	49	theme	NaCl	645:648	arg1	concentrations					650:663	NaCl concentrations	645:663	NaCl concentrations of 0-1 %	645:672	Strain ITR2T grew optimally at temperatures of 20-30 °C, at pH 6.0-7.0 and at NaCl concentrations of 0-1 %.
35580016	4	50	dep	Tumebacillus	526:537	arg1	flagellatus					539:549	flagellatus	539:549	flagellatus	539:549	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	7	51	theme	polar	978:982	arg1	phosphatidylmonomethylethanolamine					996:1029	phosphatidylmonomethylethanolamine	996:1029	phosphatidylmonomethylethanolamine	996:1029	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	51	theme	polar	978:982	arg1	lipids					984:989	the major polar lipids	968:989	the major polar lipids	968:989	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	2	52	theme	starch-degrading	74:89	arg1	strain					97:102	A starch-degrading novel strain	72:102	A starch-degrading novel strain	72:102	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	5	53	theme	20-30 °C	614:621	arg1	temperatures					598:609	temperatures	598:609	temperatures of 20-30 °C	598:621	Strain ITR2T grew optimally at temperatures of 20-30 °C, at pH 6.0-7.0 and at NaCl concentrations of 0-1 %.
35580016	6	54	theme	glutamic	776:783	arg1	acid					785:788	glutamic acid	776:788	glutamic acid	776:788	The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan).
35580016	9	55	theme	81.2 	1499:1503	arg1	%					1504:1504	78.5 and 81.2 %	1490:1504	%	1504:1504	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	55	theme	81.2 	1499:1503	arg1	values					1478:1483	the average nucleotide identity values	1446:1483	the average nucleotide identity values	1446:1483	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	6	56	theme	sole	679:682	arg1	menaquinone-7					696:708	menaquinone-7	696:708	menaquinone-7	696:708	The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan).
35580016	6	56	theme	sole	679:682	arg1	quinone					684:690	The sole quinone	675:690	The sole quinone	675:690	The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan).
35580016	8	57	theme	polar	1120:1124	arg1	lipids					1126:1131	Other polar lipids	1114:1131	Other polar lipids of the novel strain	1114:1151	Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid.
35580016	9	58	theme	hybridization	1276:1288	arg1	values					1290:1295	The in silico DNA-DNA hybridization values	1254:1295	The in silico DNA-DNA hybridization values between strain ITR2T	1254:1316	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	4	59	theme	gene	378:381	arg1	analysis					383:390	16S rRNA gene analysis	369:390	16S rRNA gene analysis	369:390	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	11	60	theme	genomic	1610:1616	arg1	data					1618:1621	the phylogenetic, chemotaxonomic and genomic data	1573:1621	the phylogenetic, chemotaxonomic and genomic data obtained in the present study	1573:1651	Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp.
35580016	11	61	theme	phylogenetic	1577:1588	arg1	data					1618:1621	the phylogenetic, chemotaxonomic and genomic data	1573:1621	the phylogenetic, chemotaxonomic and genomic data obtained in the present study	1573:1651	Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp.
35580016	4	62	theme	rRNA	373:376	arg1	analysis					383:390	16S rRNA gene analysis	369:390	16S rRNA gene analysis	369:390	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	8	63	theme	unidentified	1199:1210	arg1	phospholipids					1212:1224	three unidentified phospholipids	1193:1224	three unidentified phospholipids	1193:1224	Other polar lipids of the novel strain included, two unidentified glycolipids, three unidentified phospholipids and one unidentified lipid.
35580016	2	64	from	Republic	232:239	arg1	located					213:219	located	213:219	located	213:219	A starch-degrading novel strain, designated as strain ITR2T, was isolated from a soil sample collected from a garden near Dongguk University located in Goyang, Republic of Korea.
35580016	9	65	theme	average	1450:1456	arg1	%					1504:1504	78.5 and 81.2 %	1490:1504	%	1504:1504	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	65	theme	average	1450:1456	arg1	values					1478:1483	the average nucleotide identity values	1446:1483	the average nucleotide identity values	1446:1483	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	6	66	theme	aspartic	761:768	arg1	acid					770:773	aspartic acid	761:773	aspartic acid	761:773	The sole quinone was menaquinone-7 and the cell-wall peptidoglycan comprised alanine, aspartic acid, glutamic acid, lysine and meso-diaminopimelic acid (type-A1γ peptidoglycan).
35580016	7	67	theme	novel	889:893	arg1	strain					895:900	the novel strain	885:900	the novel strain	885:900	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	9	68	theme	DSM	1367:1369	arg1	18773T					1371:1376	T. permanentifrigoris DSM 18773T	1345:1376	T. permanentifrigoris DSM 18773T	1345:1376	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	9	68	theme	DSM	1367:1369	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T)	1322:1402	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	13	69	theme	=KCTC	1779:1783	arg1	114753T					1797:1803	=KCTC 43280T=NBRC 114753T	1779:1803	=KCTC 43280T=NBRC 114753T	1779:1803	The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
35580016	13	69	theme	=KCTC	1779:1783	arg1	ITR2T					1772:1776	ITR2T	1772:1776	ITR2T (=KCTC 43280T=NBRC 114753T)	1772:1804	The type strain is ITR2T (=KCTC 43280T=NBRC 114753T).
35580016	9	70	theme	21.3	1409:1412	arg1	%					1423:1423	21.3 and 23.9 %	1409:1423	%	1423:1423	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	11	71	theme	chemotaxonomic	1591:1604	arg1	data					1618:1621	the phylogenetic, chemotaxonomic and genomic data	1573:1621	the phylogenetic, chemotaxonomic and genomic data obtained in the present study	1573:1651	Based on the phylogenetic, chemotaxonomic and genomic data obtained in the present study, we propose Tumebacillus amylolyticus sp.
35580016	10	72	theme	DNA	1533:1535	arg1	content					1541:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content was 54.9 mol%.
35580016	10	72	theme	DNA	1533:1535	arg1	%					1561:1561	54.9 mol%	1553:1561	54.9 mol%	1553:1561	The genomic DNA G+C content was 54.9 mol%.
35580016	7	73	theme	strain	895:900	arg1	C16 					907:910	C16 	907:910	C16 	907:910	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	73	theme	strain	895:900	arg1	acids					869:873	The major fatty acids	853:873	The major fatty acids (>10%) of the novel strain	853:900	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	7	73	theme	strain	895:900	arg1	%					879:879	>10%	876:879	>10%	876:879	The major fatty acids (>10%) of the novel strain were C16 : 0, iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid.
35580016	4	74	with	clade	437:441	arg1	9.5T					485:488	Tumebacillus permanentifrigoris Eur1 9.5T	448:488	Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %)	448:497	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	4	74	with	clade	437:441	arg1	%					496:496	98.3 %	491:496	98.3 %	491:496	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	10	75	theme	G+C	1537:1539	arg1	content					1541:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content	1521:1547	The genomic DNA G+C content was 54.9 mol%.
35580016	10	75	theme	G+C	1537:1539	arg1	%					1561:1561	54.9 mol%	1553:1561	54.9 mol%	1553:1561	The genomic DNA G+C content was 54.9 mol%.
35580016	4	76	theme	Tumebacillus	448:459	arg1	9.5T					485:488	Tumebacillus permanentifrigoris Eur1 9.5T	448:488	Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %)	448:497	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	4	76	theme	Tumebacillus	448:459	arg1	%					496:496	98.3 %	491:496	98.3 %	491:496	Moreover, 16S rRNA gene analysis revealed the novel strain to form a separate clade with Tumebacillus permanentifrigoris Eur1 9.5T (98.3 %) while also clustering with Tumebacillus flagellatus GST4T (97.9 %).
35580016	9	77	dep	T.	1382:1383	arg1	flagellatus					1385:1395	flagellatus	1385:1395	flagellatus	1385:1395	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35580016	3	78	theme	peritrichous	336:347	arg1	flagella					349:356	peritrichous flagella	336:356	peritrichous flagella	336:356	The strain was identified as Gram-stain-positive, rod-shaped, and motile by means of peritrichous flagella.
35580016	9	79	theme	DNA-DNA	1268:1274	arg1	hybridization					1276:1288	The in silico DNA-DNA hybridization	1254:1288	The in silico DNA-DNA hybridization values between strain ITR2T	1254:1316	The in silico DNA-DNA hybridization values between strain ITR2T and its reference strains (T. permanentifrigoris DSM 18773T and T. flagellatus GST4T) were 21.3 and 23.9 %, respectively, while the average nucleotide identity values were 78.5 and 81.2 %, respectively.
35565850	8	0	theme	calcium	1021:1027	arg1	homeostasis					1039:1049	calcium phosphate homeostasis	1021:1049	calcium phosphate homeostasis	1021:1049	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	4	1	from	point	615:619	arg1	safe					563:566	safe	563:566	safe	563:566	GMP seems to be safe from both the microbiological and the clinical point of view.
35565850	0	2	from	Safety	18:23	arg1	Patients					61:68	Patients	61:68	Patients with Phenylketonuria	61:89	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	0	2	from	Safety	18:23	arg1	Microbiota					47:56	Gut Microbiota	43:56	Gut Microbiota	43:56	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	9	3	from	role	1234:1237	arg1	diet					1202:1205	the PKU diet	1194:1205	the PKU diet	1194:1205	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	9	3	from	role	1234:1237	arg1	taxa					1251:1254	specific taxa	1242:1254	specific taxa	1242:1254	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	8	4	theme	vitamin	1095:1101	arg1	D					1103:1103	plasmatic vitamin D	1085:1103	plasmatic vitamin D	1085:1103	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	1	5	theme	good	144:147	arg1	source					169:174	a good alternative protein source	142:174	a good alternative protein source	142:174	Glycomacropeptide (GMP) represents a good alternative protein source in Phenylketonuria (PKU).
35565850	9	6	theme	dramatic	1272:1279	arg1	changes					1281:1287	dramatic changes	1272:1287	dramatic changes in the commensal microbiota	1272:1315	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	8	7	theme	homeostasis	1039:1049	arg1	amelioration					1005:1016	an amelioration	1002:1016	an amelioration of calcium phosphate homeostasis	1002:1049	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	7	8	theme	significant	854:864	arg1	impact					866:871	a significant impact	852:871	a significant impact on both metabolic control	852:897	Clinically, GMP intake did not show a significant impact on both metabolic control, as phenylalanine values were kept below the age target and nutritional parameters.
35565850	0	9	dep	Study	100:104	arg1	Safety					18:23	Glycomacropeptide Safety	0:23	Glycomacropeptide Safety	0:23	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	0	9	dep	Study	100:104	arg1	Effect					33:38	Its Effect	29:38	Its Effect on Gut Microbiota in Patients with Phenylketonuria	29:89	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	8	10	located	observed	1055:1062	arg1	hand					996:999	the other hand	986:999	the other hand	986:999	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	8	10	located	observed	1055:1062	arg2	amelioration					1005:1016	an amelioration	1002:1016	an amelioration of calcium phosphate homeostasis	1002:1049	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	8	11	theme	phosphate	1029:1037	arg1	homeostasis					1039:1049	calcium phosphate homeostasis	1021:1049	calcium phosphate homeostasis	1021:1049	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	5	12	from	effect	721:726	arg1	spp					766:768	the butyrate-producer Agathobacter spp	731:768	the butyrate-producer Agathobacter spp	731:768	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	8	13	from	decrease	1111:1118	arg1	phosphatase					1132:1142	alkaline phosphatase	1123:1142	alkaline phosphatase	1123:1142	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	8	13	from	decrease	1111:1118	arg1	D					1103:1103	plasmatic vitamin D	1085:1103	plasmatic vitamin D	1085:1103	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	0	14	with	Patients	61:68	arg1	Phenylketonuria					75:89	Phenylketonuria	75:89	Phenylketonuria	75:89	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	3	15	theme	bacterial	466:474	arg1	composition					476:486	their bacterial composition	460:486	their bacterial composition	460:486	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	9	16	theme	safe	1174:1177	arg1	GMP					1165:1167	GMP	1165:1167	GMP	1165:1167	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	9	16	theme	safe	1174:1177	arg1	alternative					1179:1189	a safe alternative	1172:1189	a safe alternative in the PKU diet	1172:1205	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	5	17	theme	butyrate-producer	735:751	arg1	spp					766:768	the butyrate-producer Agathobacter spp	731:768	the butyrate-producer Agathobacter spp	731:768	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	4	18	from	microbiological	582:596	arg1	safe					563:566	safe	563:566	safe	563:566	GMP seems to be safe from both the microbiological and the clinical point of view.
35565850	1	19	theme	alternative	149:159	arg1	source					169:174	a good alternative protein source	142:174	a good alternative protein source	142:174	Glycomacropeptide (GMP) represents a good alternative protein source in Phenylketonuria (PKU).
35565850	0	20	theme	Pilot	94:98	arg1	Study					100:104	A Pilot Study	92:104	A Pilot Study	92:104	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	6	21	theme	lesser	781:786	arg1	extent					788:793	a lesser extent	779:793	a lesser extent	779:793	and, to a lesser extent, of Subdoligranulum.
35565850	8	22	theme	plasmatic	1085:1093	arg1	D					1103:1103	plasmatic vitamin D	1085:1103	plasmatic vitamin D	1085:1103	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	3	23	theme	16S	328:330	arg1	sequencing					337:346	the 16S rRNA sequencing	324:346	the 16S rRNA sequencing	324:346	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	1	24	theme	protein	161:167	arg1	source					169:174	a good alternative protein source	142:174	a good alternative protein source	142:174	Glycomacropeptide (GMP) represents a good alternative protein source in Phenylketonuria (PKU).
35565850	5	25	theme	specific	702:709	arg1	effect					721:726	a specific prebiotic effect	700:726	a specific prebiotic effect on the butyrate-producer Agathobacter spp	700:768	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	0	26	from	Effect	33:38	arg1	Patients					61:68	Patients	61:68	Patients with Phenylketonuria	61:89	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	0	26	from	Effect	33:38	arg1	Microbiota					47:56	Gut Microbiota	43:56	Gut Microbiota	43:56	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	7	27	theme	nutritional	959:969	arg1	parameters					971:980	nutritional parameters	959:980	nutritional parameters	959:980	Clinically, GMP intake did not show a significant impact on both metabolic control, as phenylalanine values were kept below the age target and nutritional parameters.
35565850	3	28	theme	clinical	492:499	arg1	parameters					501:510	clinical parameters	492:510	clinical parameters	492:510	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	0	29	theme	Glycomacropeptide	0:16	arg1	Safety					18:23	Glycomacropeptide Safety	0:23	Glycomacropeptide Safety	0:23	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	9	30	from	alternative	1179:1189	arg1	diet					1202:1205	the PKU diet	1194:1205	the PKU diet	1194:1205	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	9	30	from	alternative	1179:1189	arg1	taxa					1251:1254	specific taxa	1242:1254	specific taxa	1242:1254	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	8	31	theme	alkaline	1123:1130	arg1	phosphatase					1132:1142	alkaline phosphatase	1123:1142	alkaline phosphatase	1123:1142	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	5	32	theme	prebiotic	711:719	arg1	effect					721:726	a specific prebiotic effect	700:726	a specific prebiotic effect on the butyrate-producer Agathobacter spp	700:768	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	9	33	from	changes	1281:1287	arg1	microbiota					1306:1315	the commensal microbiota	1292:1315	the commensal microbiota	1292:1315	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	7	34	theme	GMP	828:830	arg1	intake					832:837	GMP intake	828:837	GMP intake	828:837	Clinically, GMP intake did not show a significant impact on both metabolic control, as phenylalanine values were kept below the age target and nutritional parameters.
35565850	2	35	theme	prebiotic	253:261	arg1	role					263:266	a prebiotic role	251:266	a prebiotic role	251:266	In a mouse model, it has been suggested to exert a prebiotic role on beneficial gut bacteria.
35565850	3	36	theme	GMP	386:388	arg1	supplementation					390:404	GMP supplementation	386:404	GMP supplementation	386:404	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	7	37	theme	metabolic	881:889	arg1	control					891:897	both metabolic control	876:897	both metabolic control	876:897	Clinically, GMP intake did not show a significant impact on both metabolic control, as phenylalanine values were kept below the age target and nutritional parameters.
35565850	7	38	from	impact	866:871	arg1	control					891:897	both metabolic control	876:897	both metabolic control	876:897	Clinically, GMP intake did not show a significant impact on both metabolic control, as phenylalanine values were kept below the age target and nutritional parameters.
35565850	3	39	theme	supplementation	390:404	arg1	months					376:381	6 months	374:381	6 months of GMP supplementation	374:404	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	5	40	theme	gut	681:683	arg1	microbiota					685:694	the gut microbiota	677:694	the gut microbiota	677:694	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	5	41	theme	Agathobacter	753:764	arg1	spp					766:768	the butyrate-producer Agathobacter spp	731:768	the butyrate-producer Agathobacter spp	731:768	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	2	42	theme	mouse	207:211	arg1	model					213:217	a mouse model	205:217	a mouse model	205:217	In a mouse model, it has been suggested to exert a prebiotic role on beneficial gut bacteria.
35565850	3	43	theme	months	376:381	arg1	effect					364:369	the effect	360:369	the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients	360:447	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	4	44	theme	clinical	606:613	arg1	point					615:619	the clinical point	602:619	the clinical point of view	602:627	GMP seems to be safe from both the microbiological and the clinical point of view.
35565850	7	45	theme	age	944:946	arg1	target					948:953	the age target	940:953	the age target	940:953	Clinically, GMP intake did not show a significant impact on both metabolic control, as phenylalanine values were kept below the age target and nutritional parameters.
35565850	3	46	theme	rRNA	332:335	arg1	sequencing					337:346	the 16S rRNA sequencing	324:346	the 16S rRNA sequencing	324:346	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	9	47	theme	possible	1215:1222	arg1	role					1234:1237	its possible prebiotic role	1211:1237	its possible prebiotic role on specific taxa	1211:1254	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	8	48	theme	other	990:994	arg1	hand					996:999	the other hand	986:999	the other hand	986:999	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	9	49	theme	PKU	1198:1200	arg1	diet					1202:1205	the PKU diet	1194:1205	the PKU diet	1194:1205	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	7	50	theme	phenylalanine	903:915	arg1	values					917:922	phenylalanine values	903:922	phenylalanine values	903:922	Clinically, GMP intake did not show a significant impact on both metabolic control, as phenylalanine values were kept below the age target and nutritional parameters.
35565850	3	51	theme	gut	413:415	arg1	microbiota					417:426	the gut microbiota	409:426	the gut microbiota of nine PKU patients	409:447	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	0	52	theme	Gut	43:45	arg1	Microbiota					47:56	Gut Microbiota	43:56	Gut Microbiota	43:56	Glycomacropeptide Safety and Its Effect on Gut Microbiota in Patients with Phenylketonuria: A Pilot Study.
35565850	9	53	theme	prebiotic	1224:1232	arg1	role					1234:1237	its possible prebiotic role	1211:1237	its possible prebiotic role on specific taxa	1211:1254	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	4	54	theme	view	624:627	arg1	point					615:619	the clinical point	602:619	the clinical point of view	602:627	GMP seems to be safe from both the microbiological and the clinical point of view.
35565850	2	55	theme	gut	282:284	arg1	bacteria					286:293	beneficial gut bacteria	271:293	beneficial gut bacteria	271:293	In a mouse model, it has been suggested to exert a prebiotic role on beneficial gut bacteria.
35565850	2	56	theme	beneficial	271:280	arg1	bacteria					286:293	beneficial gut bacteria	271:293	beneficial gut bacteria	271:293	In a mouse model, it has been suggested to exert a prebiotic role on beneficial gut bacteria.
35565850	9	57	theme	specific	1242:1249	arg1	taxa					1251:1254	specific taxa	1242:1254	specific taxa	1242:1254	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	5	58	theme	dramatic	657:664	arg1	changes					666:672	dramatic changes	657:672	dramatic changes in the gut microbiota	657:694	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	5	59	from	changes	666:672	arg1	microbiota					685:694	the gut microbiota	677:694	the gut microbiota	677:694	Indeed, we did not observe dramatic changes in the gut microbiota but a specific prebiotic effect on the butyrate-producer Agathobacter spp.
35565850	3	60	from	effect	364:369	arg1	microbiota					417:426	the gut microbiota	409:426	the gut microbiota of nine PKU patients	409:447	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	3	61	theme	PKU	436:438	arg1	patients					440:447	nine PKU patients	431:447	nine PKU patients	431:447	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
35565850	9	62	theme	commensal	1296:1304	arg1	microbiota					1306:1315	the commensal microbiota	1292:1315	the commensal microbiota	1292:1315	Our results suggest GMP as a safe alternative in the PKU diet and its possible prebiotic role on specific taxa without causing dramatic changes in the commensal microbiota.
35565850	8	63	from	increase	1073:1080	arg1	phosphatase					1132:1142	alkaline phosphatase	1123:1142	alkaline phosphatase	1123:1142	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	8	63	from	increase	1073:1080	arg1	D					1103:1103	plasmatic vitamin D	1085:1103	plasmatic vitamin D	1085:1103	On the other hand, an amelioration of calcium phosphate homeostasis was observed, with an increase in plasmatic vitamin D and a decrease in alkaline phosphatase.
35565850	3	64	theme	patients	440:447	arg1	microbiota					417:426	the gut microbiota	409:426	the gut microbiota of nine PKU patients	409:447	In this study, we performed the 16S rRNA sequencing to evaluate the effect of 6 months of GMP supplementation on the gut microbiota of nine PKU patients, comparing their bacterial composition and clinical parameters before and after the intervention.
36398593	0	0	theme	β-glucan	70:77	arg1	effect					33:38	synergistic effect	21:38	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.	0:137	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	5	1	theme	regulatory	691:700	arg1	effects					702:708	the regulatory effects	687:708	the regulatory effects of OPC and OBG	687:723	There was no significant difference in the regulatory effects of OPC and OBG (p > 0.05).
36398593	7	2	theme	PCR	936:938	arg1	study					950:954	The real-time quantitative PCR (RT-qPCR) study	909:954	The real-time quantitative PCR (RT-qPCR) study	909:954	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	8	3	theme	liver	1139:1143	arg1	stress					1155:1160	liver oxidative stress	1139:1160	liver lipid deposition as well as liver oxidative stress (p < 0.05)	1105:1171	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	8	3	theme	liver	1139:1143	arg1	<					1165:1165	p < 0.05	1163:1170	p < 0.05	1163:1170	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	4	4	theme	fasting	537:543	arg1	FBG					560:562	FBG	560:562	FBG	560:562	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	4	4	theme	fasting	537:543	arg1	glucose					551:557	fasting blood glucose	537:557	fasting blood glucose (FBG)	537:563	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	10	5	theme	fed	1430:1432	arg1	mice					1434:1437	the OPC and OBG fed mice	1414:1437	mice	1434:1437	OPC + OBG significantly increased the abundance of Bacteroidetes and reduced the abundance of Firmicutes (p < 0.05) compared with the OPC and OBG fed mice.
36398593	10	6	theme	OBG	1426:1428	arg1	mice					1434:1437	the OPC and OBG fed mice	1414:1437	mice	1434:1437	OPC + OBG significantly increased the abundance of Bacteroidetes and reduced the abundance of Firmicutes (p < 0.05) compared with the OPC and OBG fed mice.
36398593	4	7	theme	HFD	633:635	arg1	mice					642:645	high-fat-diet (HFD) fed mice	618:645	high-fat-diet (HFD) fed mice	618:645	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	6	8	theme	blood	847:851	arg1	p					862:862	p < 0.01	862:869	p < 0.01	862:869	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	8	theme	blood	847:851	arg1	glucose					853:859	blood glucose	847:859	blood glucose (p < 0.01)	847:870	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	3	9	theme	oat	354:356	arg1	components					342:351	whole grain components	330:351	whole grain components	330:351	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	2	10	theme	specific	254:261	arg1	ingredient					263:272	specific ingredient	254:272	specific ingredient	254:272	However, which specific ingredient is crucial remains unclear.
36398593	7	11	theme	real-time	913:921	arg1	RT-qPCR					941:947	RT-qPCR	941:947	RT-qPCR	941:947	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	7	11	theme	real-time	913:921	arg1	PCR					936:938	real-time quantitative PCR	913:938	The real-time quantitative PCR (RT-qPCR) study	909:954	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	9	12	theme	gut	1223:1225	arg1	phenotype					1248:1256	the gut microbiota community phenotype	1219:1256	the gut microbiota community phenotype	1219:1256	In addition, OPC + OBG combination regulated the gut microbiota community phenotype and increased probiotics.
36398593	7	13	theme	related	1018:1024	arg1	expression					1007:1016	mRNA expression	1002:1016	mRNA expression related to lipid metabolism	1002:1044	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	11	14	theme	lipid	1524:1528	arg1	metabolism					1530:1539	lipid metabolism	1524:1539	lipid metabolism	1524:1539	In conclusion, OPC + OBG has a synergistic effect in alleviating hyperlipidemia via lipid metabolism and gut microbiota composition.
36398593	3	15	theme	phenolic	358:365	arg1	OPC					378:380	OPC	378:380	OPC	378:380	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	3	15	theme	phenolic	358:365	arg1	compounds					367:375	phenolic compounds	358:375	phenolic compounds (OPC)	358:381	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	7	16	theme	quantitative	923:934	arg1	RT-qPCR					941:947	RT-qPCR	941:947	RT-qPCR	941:947	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	7	16	theme	quantitative	923:934	arg1	PCR					936:938	real-time quantitative PCR	913:938	The real-time quantitative PCR (RT-qPCR) study	909:954	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	8	17	theme	oxidative	1145:1153	arg1	stress					1155:1160	liver oxidative stress	1139:1160	liver lipid deposition as well as liver oxidative stress (p < 0.05)	1105:1171	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	8	17	theme	oxidative	1145:1153	arg1	<					1165:1165	p < 0.05	1163:1170	p < 0.05	1163:1170	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	12	18	theme	whole	1648:1652	arg1	grains					1654:1659	whole grains	1648:1659	whole grains	1648:1659	This finding also provided a potential justification for the advantages of whole grains in preventing hyperlipidemia.
36398593	4	19	theme	blood	545:549	arg1	FBG					560:562	FBG	560:562	FBG	560:562	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	4	19	theme	blood	545:549	arg1	glucose					551:557	fasting blood glucose	537:557	fasting blood glucose (FBG)	537:563	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	0	20	theme	gut	101:103	arg1	microbiota					105:114	gut microbiota	101:114	gut microbiota	101:114	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	8	21	theme	p	1163:1163	arg1	stress					1155:1160	liver oxidative stress	1139:1160	liver lipid deposition as well as liver oxidative stress (p < 0.05)	1105:1171	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	8	21	theme	p	1163:1163	arg1	<					1165:1165	p < 0.05	1163:1170	p < 0.05	1163:1170	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	9	22	theme	microbiota	1227:1236	arg1	phenotype					1248:1256	the gut microbiota community phenotype	1219:1256	the gut microbiota community phenotype	1219:1256	In addition, OPC + OBG combination regulated the gut microbiota community phenotype and increased probiotics.
36398593	4	23	theme	lipid	602:606	arg1	levels					608:613	hepatic lipid levels	594:613	hepatic lipid levels	594:613	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	5	24	theme	OBG	721:723	arg1	effects					702:708	the regulatory effects	687:708	the regulatory effects of OPC and OBG	687:723	There was no significant difference in the regulatory effects of OPC and OBG (p > 0.05).
36398593	0	25	from	effect	33:38	arg1	hyperlipidemia					82:95	hyperlipidemia	82:95	hyperlipidemia via gut microbiota in high-fat-diet mice	82:136	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	6	26	theme	glucose	853:859	arg1	levels					890:895	the blood glucose (p < 0.01) and lipid profile levels	843:895	the blood glucose (p < 0.01) and lipid profile levels (p < 0.01)	843:906	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	26	theme	glucose	853:859	arg1	<					900:900	p < 0.01	898:905	p < 0.01	898:905	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	5	27	theme	p	726:726	arg1	>					728:728	p > 0.05	726:733	p > 0.05	726:733	There was no significant difference in the regulatory effects of OPC and OBG (p > 0.05).
36398593	11	28	theme	gut	1545:1547	arg1	composition					1560:1570	gut microbiota composition	1545:1570	gut microbiota composition	1545:1570	In conclusion, OPC + OBG has a synergistic effect in alleviating hyperlipidemia via lipid metabolism and gut microbiota composition.
36398593	4	29	theme	hepatic	594:600	arg1	levels					608:613	hepatic lipid levels	594:613	hepatic lipid levels	594:613	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	0	30	theme	grain	6:10	arg1	benefit					12:18	Whole grain benefit	0:18	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.	0:137	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	0	31	theme	compounds	56:64	arg1	effect					33:38	synergistic effect	21:38	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.	0:137	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	12	32	theme	potential	1602:1610	arg1	justification					1612:1624	a potential justification	1600:1624	a potential justification for the advantages of whole grains in preventing hyperlipidemia	1600:1688	This finding also provided a potential justification for the advantages of whole grains in preventing hyperlipidemia.
36398593	1	33	theme	whole	178:182	arg1	oats					190:193	whole grain oats	178:193	whole grain oats	178:193	Increasing evidence has confirmed that whole grain oats are effective in regulating hyperlipidemia.
36398593	0	34	theme	Whole	0:4	arg1	benefit					12:18	Whole grain benefit	0:18	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.	0:137	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	8	35	theme	Histopathological	1047:1063	arg1	analysis					1065:1072	Histopathological analysis	1047:1072	Histopathological analysis	1047:1072	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	11	36	theme	synergistic	1471:1481	arg1	effect					1483:1488	a synergistic effect	1469:1488	a synergistic effect	1469:1488	In conclusion, OPC + OBG has a synergistic effect in alleviating hyperlipidemia via lipid metabolism and gut microbiota composition.
36398593	1	37	theme	grain	184:188	arg1	oats					190:193	whole grain oats	178:193	whole grain oats	178:193	Increasing evidence has confirmed that whole grain oats are effective in regulating hyperlipidemia.
36398593	10	38	theme	p	1390:1390	arg1	<					1392:1392	p < 0.05	1390:1397	p < 0.05	1390:1397	OPC + OBG significantly increased the abundance of Bacteroidetes and reduced the abundance of Firmicutes (p < 0.05) compared with the OPC and OBG fed mice.
36398593	10	38	theme	p	1390:1390	arg1	Firmicutes					1378:1387	Firmicutes	1378:1387	Firmicutes (p < 0.05)	1378:1398	OPC + OBG significantly increased the abundance of Bacteroidetes and reduced the abundance of Firmicutes (p < 0.05) compared with the OPC and OBG fed mice.
36398593	8	39	theme	lipid	1111:1115	arg1	deposition					1117:1126	liver lipid deposition	1105:1126	liver lipid deposition as well as liver oxidative stress (p < 0.05)	1105:1171	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	6	40	theme	profile	882:888	arg1	levels					890:895	the blood glucose (p < 0.01) and lipid profile levels	843:895	the blood glucose (p < 0.01) and lipid profile levels (p < 0.01)	843:906	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	40	theme	profile	882:888	arg1	<					900:900	p < 0.01	898:905	p < 0.01	898:905	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	11	41	theme	microbiota	1549:1558	arg1	composition					1560:1570	gut microbiota composition	1545:1570	gut microbiota composition	1545:1570	In conclusion, OPC + OBG has a synergistic effect in alleviating hyperlipidemia via lipid metabolism and gut microbiota composition.
36398593	9	42	theme	OBG	1193:1195	arg1	combination					1197:1207	OBG combination	1193:1207	OBG combination	1193:1207	In addition, OPC + OBG combination regulated the gut microbiota community phenotype and increased probiotics.
36398593	6	43	theme	OBG	764:766	arg1	combination					741:751	The combination	737:751	The combination of OPC and OBG (OPC + OBG)	737:778	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	44	theme	lipid	876:880	arg1	profile					882:888	lipid profile	876:888	lipid profile	876:888	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	7	45	theme	mRNA	1002:1005	arg1	expression					1007:1016	mRNA expression	1002:1016	mRNA expression related to lipid metabolism	1002:1044	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	4	46	theme	body	521:524	arg1	weight					526:531	the body weight	517:531	the body weight	517:531	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	12	47	theme	grains	1654:1659	arg1	advantages					1634:1643	the advantages	1630:1643	the advantages of whole grains in preventing hyperlipidemia	1630:1688	This finding also provided a potential justification for the advantages of whole grains in preventing hyperlipidemia.
36398593	9	48	theme	community	1238:1246	arg1	phenotype					1248:1256	the gut microbiota community phenotype	1219:1256	the gut microbiota community phenotype	1219:1256	In addition, OPC + OBG combination regulated the gut microbiota community phenotype and increased probiotics.
36398593	0	49	theme	synergistic	21:31	arg1	effect					33:38	synergistic effect	21:38	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.	0:137	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	6	50	theme	p	821:821	arg1	weight					813:818	the body weight	804:818	the body weight (p < 0.01)	804:829	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	50	theme	p	821:821	arg1	<					823:823	p < 0.01	821:828	p < 0.01	821:828	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	0	51	theme	high-fat-diet	119:131	arg1	mice					133:136	high-fat-diet mice	119:136	high-fat-diet mice	119:136	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	6	52	theme	OPC	756:758	arg1	combination					741:751	The combination	737:751	The combination of OPC and OBG (OPC + OBG)	737:778	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	3	53	theme	lipid	419:423	arg1	metabolism					425:434	lipid metabolism	419:434	lipid metabolism	419:434	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	0	54	theme	oat	43:45	arg1	compounds					56:64	oat phenolic compounds	43:64	oat phenolic compounds	43:64	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	6	55	dep	OPC	756:758	arg1	OBG					775:777	OBG	775:777	OBG	775:777	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	55	dep	OPC	756:758	arg1	OPC					769:771	OPC	769:771	OPC	769:771	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	10	56	theme	Firmicutes	1378:1387	arg1	abundance					1365:1373	the abundance	1361:1373	the abundance of Firmicutes (p < 0.05)	1361:1398	OPC + OBG significantly increased the abundance of Bacteroidetes and reduced the abundance of Firmicutes (p < 0.05) compared with the OPC and OBG fed mice.
36398593	0	57	theme	phenolic	47:54	arg1	compounds					56:64	oat phenolic compounds	43:64	oat phenolic compounds	43:64	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	5	58	dep	was	654:656	arg1	>					728:728	p > 0.05	726:733	p > 0.05	726:733	There was no significant difference in the regulatory effects of OPC and OBG (p > 0.05).
36398593	8	59	theme	liver	1105:1109	arg1	deposition					1117:1126	liver lipid deposition	1105:1126	liver lipid deposition as well as liver oxidative stress (p < 0.05)	1105:1171	Histopathological analysis showed that OPC + OBG improved liver lipid deposition as well as liver oxidative stress (p < 0.05).
36398593	3	60	theme	oat	386:388	arg1	OBG					400:402	OBG	400:402	OBG	400:402	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	3	60	theme	oat	386:388	arg1	β-glucan					390:397	oat β-glucan	386:397	oat β-glucan (OBG)	386:403	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	7	61	theme	lipid	1029:1033	arg1	metabolism					1035:1044	lipid metabolism	1029:1044	lipid metabolism	1029:1044	The real-time quantitative PCR (RT-qPCR) study revealed that OPC + OBG significantly altered mRNA expression related to lipid metabolism.
36398593	10	62	theme	Bacteroidetes	1335:1347	arg1	abundance					1322:1330	the abundance	1318:1330	the abundance of Bacteroidetes	1318:1347	OPC + OBG significantly increased the abundance of Bacteroidetes and reduced the abundance of Firmicutes (p < 0.05) compared with the OPC and OBG fed mice.
36398593	4	63	theme	fed	638:640	arg1	mice					642:645	high-fat-diet (HFD) fed mice	618:645	high-fat-diet (HFD) fed mice	618:645	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	6	64	theme	body	808:811	arg1	weight					813:818	the body weight	804:818	the body weight (p < 0.01)	804:829	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	64	theme	body	808:811	arg1	<					823:823	p < 0.01	821:828	p < 0.01	821:828	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	3	65	dep	oat	354:356	arg1	OPC					378:380	OPC	378:380	OPC	378:380	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	3	65	dep	oat	354:356	arg1	compounds					367:375	phenolic compounds	358:375	phenolic compounds (OPC)	358:381	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	3	65	dep	oat	354:356	arg1	β-glucan					390:397	oat β-glucan	386:397	oat β-glucan (OBG)	386:403	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	3	65	dep	oat	354:356	arg1	OBG					400:402	OBG	400:402	OBG	400:402	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	3	66	theme	gut	440:442	arg1	microbiota					444:453	gut microbiota	440:453	gut microbiota	440:453	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	11	67	contain	has	1465:1467	arg2	effect					1483:1488	a synergistic effect	1469:1488	a synergistic effect	1469:1488	In conclusion, OPC + OBG has a synergistic effect in alleviating hyperlipidemia via lipid metabolism and gut microbiota composition.
36398593	11	67	contain	has	1465:1467	arg1	OBG					1461:1463	OBG	1461:1463	OBG	1461:1463	In conclusion, OPC + OBG has a synergistic effect in alleviating hyperlipidemia via lipid metabolism and gut microbiota composition.
36398593	11	67	contain	has	1465:1467	arg1	OPC					1455:1457	OPC	1455:1457	OPC	1455:1457	In conclusion, OPC + OBG has a synergistic effect in alleviating hyperlipidemia via lipid metabolism and gut microbiota composition.
36398593	5	68	theme	significant	661:671	arg1	difference					673:682	no significant difference	658:682	no significant difference	658:682	There was no significant difference in the regulatory effects of OPC and OBG (p > 0.05).
36398593	0	69	from	hyperlipidemia	82:95	arg1	mice					133:136	high-fat-diet mice	119:136	high-fat-diet mice	119:136	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	0	70	dep	benefit	12:18	arg1	effect					33:38	synergistic effect	21:38	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.	0:137	Whole grain benefit: synergistic effect of oat phenolic compounds and β-glucan on hyperlipidemia via gut microbiota in high-fat-diet mice.
36398593	4	71	theme	high-fat-diet	618:630	arg1	mice					642:645	high-fat-diet (HFD) fed mice	618:645	high-fat-diet (HFD) fed mice	618:645	The experiment unveiled that OPC and/or OBG not only reduced the body weight and fasting blood glucose (FBG) but also regulated serum and hepatic lipid levels in high-fat-diet (HFD) fed mice.
36398593	6	72	theme	<	864:864	arg1	p					862:862	p < 0.01	862:869	p < 0.01	862:869	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	72	theme	<	864:864	arg1	glucose					853:859	blood glucose	847:859	blood glucose (p < 0.01)	847:870	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	3	73	theme	whole	330:334	arg1	components					342:351	whole grain components	330:351	whole grain components	330:351	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	6	74	theme	p	898:898	arg1	levels					890:895	the blood glucose (p < 0.01) and lipid profile levels	843:895	the blood glucose (p < 0.01) and lipid profile levels (p < 0.01)	843:906	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	6	74	theme	p	898:898	arg1	<					900:900	p < 0.01	898:905	p < 0.01	898:905	The combination of OPC and OBG (OPC + OBG) significantly decreased the body weight (p < 0.01) and reduced the blood glucose (p < 0.01) and lipid profile levels (p < 0.01).
36398593	3	75	theme	grain	336:340	arg1	components					342:351	whole grain components	330:351	whole grain components	330:351	This study focused on which whole grain components, oat phenolic compounds (OPC) or oat β-glucan (OBG), can regulate lipid metabolism and gut microbiota.
36398593	5	76	theme	OPC	713:715	arg1	effects					702:708	the regulatory effects	687:708	the regulatory effects of OPC and OBG	687:723	There was no significant difference in the regulatory effects of OPC and OBG (p > 0.05).
36398593	1	77	theme	Increasing	139:148	arg1	evidence					150:157	Increasing evidence	139:157	Increasing evidence	139:157	Increasing evidence has confirmed that whole grain oats are effective in regulating hyperlipidemia.
34907602	3	0	from	relationship	425:436	arg1	patients					502:509	colorectal cancer (CRC) patients	478:509	colorectal cancer (CRC) patients	478:509	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	13	1	theme	muscularis	1823:1832	arg1	inflammation					1834:1845	intestinal muscularis inflammation	1812:1845	intestinal muscularis inflammation	1812:1845	CONCLUSION Lipopolysaccharide increases intestinal muscularis inflammation via activation of p38 signaling, which aggravates POI.
34907602	7	2	theme	pro-inflammatory	994:1009	arg1	composition					1025:1035	a unique pro-inflammatory gut microbial composition	985:1035	a unique pro-inflammatory gut microbial composition	985:1035	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	1	3	theme	abdominal	182:190	arg1	surgery					192:198	abdominal surgery	182:198	abdominal surgery	182:198	BACKGROUND AND AIM Patients undergoing abdominal surgery can develop postoperative ileus (POI).
34907602	0	4	theme	postoperative	92:104	arg1	ileus					106:110	postoperative ileus	92:110	postoperative ileus in colorectal cancer patients	92:140	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	8	5	theme	bacteria	1111:1118	arg1	Escherichia-Shigella					1144:1163	Escherichia-Shigella	1144:1163	Escherichia-Shigella	1144:1163	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	8	5	theme	bacteria	1111:1118	arg1	proportions					1082:1092	The highest proportions	1070:1092	The highest proportions of Gram-negative bacteria at the genus level	1070:1137	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	6	6	theme	causal	852:857	arg1	relationship					859:870	the causal relationship	848:870	the causal relationship between microbiota, LPS, and POI	848:903	Mice were used to mechanistically investigate the causal relationship between microbiota, LPS, and POI.
34907602	13	7	theme	intestinal	1812:1821	arg1	inflammation					1834:1845	intestinal muscularis inflammation	1812:1845	intestinal muscularis inflammation	1812:1845	CONCLUSION Lipopolysaccharide increases intestinal muscularis inflammation via activation of p38 signaling, which aggravates POI.
34907602	0	8	theme	colorectal	115:124	arg1	patients					133:140	colorectal cancer patients	115:140	colorectal cancer patients	115:140	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	9	9	theme	LPS	1274:1276	arg1	levels					1278:1283	Fecal LPS levels	1268:1283	Fecal LPS levels	1268:1283	Fecal LPS levels were significantly higher in patients with PPOI.
34907602	8	10	theme	highest	1074:1080	arg1	Escherichia-Shigella					1144:1163	Escherichia-Shigella	1144:1163	Escherichia-Shigella	1144:1163	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	8	10	theme	highest	1074:1080	arg1	proportions					1082:1092	The highest proportions	1070:1092	The highest proportions of Gram-negative bacteria at the genus level	1070:1137	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	3	11	theme	LPS-triggered	548:560	arg1	POI					562:564	LPS-triggered POI	548:564	LPS-triggered POI	548:564	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	8	12	theme	Escherichia-Shigella	1199:1218	arg1	abundance					1186:1194	the abundance	1182:1194	the abundance of Escherichia-Shigella	1182:1218	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	8	12	theme	Escherichia-Shigella	1199:1218	arg1	higher					1224:1229	higher	1224:1229	higher	1224:1229	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	14	13	theme	LPS	1932:1934	arg1	sources					1921:1927	bacterial sources	1911:1927	bacterial sources of LPS	1911:1934	Removing bacterial sources of LPS during the perioperative period is promising for the prophylactic treatment of PPOI.
34907602	3	14	theme	POI	562:564	arg1	mechanisms					534:543	underlying mechanisms	523:543	underlying mechanisms of LPS-triggered POI	523:564	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	4	15	theme	colorectal	605:614	arg1	resection					616:624	colorectal resection	605:624	colorectal resection	605:624	METHODS Sixty CRC patients undergoing colorectal resection were included.
34907602	13	16	theme	CONCLUSION	1772:1781	arg1	Lipopolysaccharide					1783:1800	CONCLUSION Lipopolysaccharide	1772:1800	CONCLUSION Lipopolysaccharide	1772:1800	CONCLUSION Lipopolysaccharide increases intestinal muscularis inflammation via activation of p38 signaling, which aggravates POI.
34907602	5	17	theme	Bacterial	641:649	arg1	communities					651:661	Bacterial communities	641:661	Bacterial communities from fecal samples	641:680	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	14	18	theme	perioperative	1947:1959	arg1	period					1961:1966	the perioperative period	1943:1966	the perioperative period	1943:1966	Removing bacterial sources of LPS during the perioperative period is promising for the prophylactic treatment of PPOI.
34907602	6	19	used	used	812:815	arg2	Mice					802:805	Mice	802:805	Mice	802:805	Mice were used to mechanistically investigate the causal relationship between microbiota, LPS, and POI.
34907602	0	20	theme	cancer	126:131	arg1	patients					133:140	colorectal cancer patients	115:140	colorectal cancer patients	115:140	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	11	21	with	treatment	1581:1589	arg1	LPS					1596:1598	LPS	1596:1598	LPS	1596:1598	Inflammation and dysfunction were significantly reduced in mice treated with polymyxin B, but were worsened by treatment with LPS.
34907602	9	22	theme	Fecal	1268:1272	arg1	levels					1278:1283	Fecal LPS levels	1268:1283	Fecal LPS levels	1268:1283	Fecal LPS levels were significantly higher in patients with PPOI.
34907602	11	23	theme	polymyxin	1547:1555	arg1	B					1557:1557	polymyxin B	1547:1557	polymyxin B	1547:1557	Inflammation and dysfunction were significantly reduced in mice treated with polymyxin B, but were worsened by treatment with LPS.
34907602	8	24	theme	perioperative	1246:1258	arg1	period					1260:1265	the perioperative period	1242:1265	the perioperative period	1242:1265	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	10	25	theme	antibiotic	1358:1367	arg1	cocktail					1369:1376	an antibiotic cocktail	1355:1376	an antibiotic cocktail	1355:1376	In mice treated with an antibiotic cocktail, intestinal muscularis inflammation and intestinal dysfunction were significantly improved.
34907602	5	26	theme	fecal	668:672	arg1	samples					674:680	fecal samples	668:680	fecal samples	668:680	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	7	27	dep	RESULTS	906:912	arg1	discovered					917:926	discovered	917:926	discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period	917:1067	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	1	28	theme	postoperative	212:224	arg1	POI					233:235	POI	233:235	POI	233:235	BACKGROUND AND AIM Patients undergoing abdominal surgery can develop postoperative ileus (POI).
34907602	1	28	theme	postoperative	212:224	arg1	ileus					226:230	postoperative ileus	212:230	postoperative ileus (POI)	212:236	BACKGROUND AND AIM Patients undergoing abdominal surgery can develop postoperative ileus (POI).
34907602	4	29	theme	METHODS	567:573	arg1	patients					585:592	METHODS Sixty CRC patients	567:592	METHODS Sixty CRC patients undergoing colorectal resection	567:624	METHODS Sixty CRC patients undergoing colorectal resection were included.
34907602	8	30	theme	Gram-negative	1097:1109	arg1	bacteria					1111:1118	Gram-negative bacteria	1097:1118	Gram-negative bacteria	1097:1118	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	10	31	theme	intestinal	1379:1388	arg1	inflammation					1401:1412	intestinal muscularis inflammation	1379:1412	intestinal muscularis inflammation	1379:1412	In mice treated with an antibiotic cocktail, intestinal muscularis inflammation and intestinal dysfunction were significantly improved.
34907602	3	32	theme	study	396:400	arg1	aim					384:386	The aim	380:386	The aim of this study	380:400	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	5	33	from	samples	674:680	arg1	communities					651:661	Bacterial communities	641:661	Bacterial communities from fecal samples	641:680	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	7	34	theme	prolonged	960:968	arg1	POI					970:972	prolonged POI	960:972	prolonged POI (PPOI)	960:979	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	7	34	theme	prolonged	960:968	arg1	PPOI					975:978	PPOI	975:978	PPOI	975:978	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	9	35	with	patients	1314:1321	arg1	PPOI					1328:1331	PPOI	1328:1331	PPOI	1328:1331	Fecal LPS levels were significantly higher in patients with PPOI.
34907602	0	36	theme	p38	14:16	arg1	kinase					44:49	p38 mitogen-activated protein kinase	14:49	p38 mitogen-activated protein kinase pathway	14:57	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	4	37	theme	CRC	581:583	arg1	patients					585:592	METHODS Sixty CRC patients	567:592	METHODS Sixty CRC patients undergoing colorectal resection	567:624	METHODS Sixty CRC patients undergoing colorectal resection were included.
34907602	5	38	theme	fecal	734:738	arg1	levels					744:749	fecal LPS levels	734:749	fecal LPS levels	734:749	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	0	39	from	ileus	106:110	arg1	patients					133:140	colorectal cancer patients	115:140	colorectal cancer patients	115:140	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	2	40	theme	displaced	332:340	arg1	bacteria					342:349	displaced bacteria	332:349	displaced bacteria	332:349	Inflammation of the intestinal muscularis following intestinal manipulation may be caused by displaced bacteria or lipopolysaccharide (LPS).
34907602	12	41	theme	intestinal	1731:1740	arg1	inflammation					1742:1753	intestinal inflammation	1731:1753	intestinal inflammation	1731:1753	Moreover, LPS upregulated p38 phosphorylation in mice, and treatment with an inhibitor of p38 (SB203580) significantly alleviated intestinal inflammation and dysmotility.
34907602	3	42	theme	colorectal	478:487	arg1	CRC					497:499	CRC	497:499	CRC	497:499	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	3	42	theme	colorectal	478:487	arg1	cancer					489:494	colorectal cancer	478:494	colorectal cancer (CRC) patients	478:509	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	0	43	theme	kinase	44:49	arg1	pathway					51:57	p38 mitogen-activated protein kinase pathway	14:57	p38 mitogen-activated protein kinase pathway	14:57	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	4	44	theme	Sixty	575:579	arg1	patients					585:592	METHODS Sixty CRC patients	567:592	METHODS Sixty CRC patients undergoing colorectal resection	567:624	METHODS Sixty CRC patients undergoing colorectal resection were included.
34907602	5	45	theme	LPS	740:742	arg1	levels					744:749	fecal LPS levels	734:749	fecal LPS levels	734:749	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	2	46	theme	intestinal	259:268	arg1	muscularis					270:279	the intestinal muscularis	255:279	the intestinal muscularis	255:279	Inflammation of the intestinal muscularis following intestinal manipulation may be caused by displaced bacteria or lipopolysaccharide (LPS).
34907602	7	47	theme	CRC	933:935	arg1	patients					937:944	CRC patients	933:944	CRC patients who developed prolonged POI (PPOI)	933:979	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	0	48	theme	protein	36:42	arg1	kinase					44:49	p38 mitogen-activated protein kinase	14:49	p38 mitogen-activated protein kinase pathway	14:57	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	13	49	theme	p38	1865:1867	arg1	signaling					1869:1877	p38 signaling	1865:1877	p38 signaling	1865:1877	CONCLUSION Lipopolysaccharide increases intestinal muscularis inflammation via activation of p38 signaling, which aggravates POI.
34907602	3	50	theme	gut	446:448	arg1	microbiota					450:459	gut microbiota	446:459	gut microbiota	446:459	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	0	51	theme	mitogen-activated	18:34	arg1	kinase					44:49	p38 mitogen-activated protein kinase	14:49	p38 mitogen-activated protein kinase pathway	14:57	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	10	52	theme	intestinal	1418:1427	arg1	dysfunction					1429:1439	intestinal dysfunction	1418:1439	intestinal dysfunction	1418:1439	In mice treated with an antibiotic cocktail, intestinal muscularis inflammation and intestinal dysfunction were significantly improved.
34907602	5	53	theme	Limulus	770:776	arg1	assay					795:799	Limulus amebocyte lysate assay	770:799	Limulus amebocyte lysate assay	770:799	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	13	54	theme	signaling	1869:1877	arg1	activation					1851:1860	activation	1851:1860	activation	1851:1860	CONCLUSION Lipopolysaccharide increases intestinal muscularis inflammation via activation of p38 signaling, which aggravates POI.
34907602	5	55	theme	rRNA	708:711	arg1	sequencing					718:727	rRNA gene sequencing	708:727	rRNA gene sequencing	708:727	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	14	56	theme	prophylactic	1989:2000	arg1	treatment					2002:2010	the prophylactic treatment	1985:2010	the prophylactic treatment of PPOI	1985:2018	Removing bacterial sources of LPS during the perioperative period is promising for the prophylactic treatment of PPOI.
34907602	7	57	theme	perioperative	1048:1060	arg1	period					1062:1067	the perioperative period	1044:1067	the perioperative period	1044:1067	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	0	58	theme	pathway	51:57	arg1	Activation					0:9	Activation	0:9	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide	0:79	Activation of p38 mitogen-activated protein kinase pathway by lipopolysaccharide aggravates postoperative ileus in colorectal cancer patients.
34907602	14	59	theme	bacterial	1911:1919	arg1	sources					1921:1927	bacterial sources	1911:1927	bacterial sources of LPS	1911:1934	Removing bacterial sources of LPS during the perioperative period is promising for the prophylactic treatment of PPOI.
34907602	5	60	theme	gene	713:716	arg1	sequencing					718:727	rRNA gene sequencing	708:727	rRNA gene sequencing	708:727	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	1	61	theme	BACKGROUND	143:152	arg1	Patients					162:169	BACKGROUND AND AIM Patients	143:169	BACKGROUND AND AIM Patients undergoing abdominal surgery	143:198	BACKGROUND AND AIM Patients undergoing abdominal surgery can develop postoperative ileus (POI).
34907602	10	62	theme	muscularis	1390:1399	arg1	inflammation					1401:1412	intestinal muscularis inflammation	1379:1412	intestinal muscularis inflammation	1379:1412	In mice treated with an antibiotic cocktail, intestinal muscularis inflammation and intestinal dysfunction were significantly improved.
34907602	3	63	theme	cancer	489:494	arg1	patients					502:509	colorectal cancer (CRC) patients	478:509	colorectal cancer (CRC) patients	478:509	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	8	64	from	level	1133:1137	arg1	Escherichia-Shigella					1144:1163	Escherichia-Shigella	1144:1163	Escherichia-Shigella	1144:1163	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	8	64	from	level	1133:1137	arg1	proportions					1082:1092	The highest proportions	1070:1092	The highest proportions of Gram-negative bacteria at the genus level	1070:1137	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
34907602	12	65	theme	p38	1691:1693	arg1	inhibitor					1678:1686	an inhibitor	1675:1686	an inhibitor of p38 (SB203580)	1675:1704	Moreover, LPS upregulated p38 phosphorylation in mice, and treatment with an inhibitor of p38 (SB203580) significantly alleviated intestinal inflammation and dysmotility.
34907602	12	65	theme	p38	1691:1693	arg1	SB203580					1696:1703	SB203580	1696:1703	SB203580	1696:1703	Moreover, LPS upregulated p38 phosphorylation in mice, and treatment with an inhibitor of p38 (SB203580) significantly alleviated intestinal inflammation and dysmotility.
34907602	2	66	theme	intestinal	291:300	arg1	manipulation					302:313	intestinal manipulation	291:313	intestinal manipulation	291:313	Inflammation of the intestinal muscularis following intestinal manipulation may be caused by displaced bacteria or lipopolysaccharide (LPS).
34907602	7	67	theme	gut	1011:1013	arg1	composition					1025:1035	a unique pro-inflammatory gut microbial composition	985:1035	a unique pro-inflammatory gut microbial composition	985:1035	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	1	68	theme	AIM	158:160	arg1	Patients					162:169	BACKGROUND AND AIM Patients	143:169	BACKGROUND AND AIM Patients undergoing abdominal surgery	143:198	BACKGROUND AND AIM Patients undergoing abdominal surgery can develop postoperative ileus (POI).
34907602	12	69	with	treatment	1660:1668	arg1	inhibitor					1678:1686	an inhibitor	1675:1686	an inhibitor of p38 (SB203580)	1675:1704	Moreover, LPS upregulated p38 phosphorylation in mice, and treatment with an inhibitor of p38 (SB203580) significantly alleviated intestinal inflammation and dysmotility.
34907602	12	69	with	treatment	1660:1668	arg1	SB203580					1696:1703	SB203580	1696:1703	SB203580	1696:1703	Moreover, LPS upregulated p38 phosphorylation in mice, and treatment with an inhibitor of p38 (SB203580) significantly alleviated intestinal inflammation and dysmotility.
34907602	5	70	theme	amebocyte	778:786	arg1	assay					795:799	Limulus amebocyte lysate assay	770:799	Limulus amebocyte lysate assay	770:799	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	14	71	theme	PPOI	2015:2018	arg1	treatment					2002:2010	the prophylactic treatment	1985:2010	the prophylactic treatment of PPOI	1985:2018	Removing bacterial sources of LPS during the perioperative period is promising for the prophylactic treatment of PPOI.
34907602	12	72	theme	p38	1627:1629	arg1	phosphorylation					1631:1645	p38 phosphorylation	1627:1645	p38 phosphorylation	1627:1645	Moreover, LPS upregulated p38 phosphorylation in mice, and treatment with an inhibitor of p38 (SB203580) significantly alleviated intestinal inflammation and dysmotility.
34907602	7	73	theme	microbial	1015:1023	arg1	composition					1025:1035	a unique pro-inflammatory gut microbial composition	985:1035	a unique pro-inflammatory gut microbial composition	985:1035	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	7	74	contain	had	981:983	arg1	patients					937:944	CRC patients	933:944	CRC patients who developed prolonged POI (PPOI)	933:979	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	7	74	contain	had	981:983	arg2	composition					1025:1035	a unique pro-inflammatory gut microbial composition	985:1035	a unique pro-inflammatory gut microbial composition	985:1035	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	5	75	theme	lysate	788:793	arg1	assay					795:799	Limulus amebocyte lysate assay	770:799	Limulus amebocyte lysate assay	770:799	Bacterial communities from fecal samples were characterized by 16S rRNA gene sequencing, and fecal LPS levels were determined by Limulus amebocyte lysate assay.
34907602	2	76	theme	muscularis	270:279	arg1	Inflammation					239:250	Inflammation	239:250	Inflammation of the intestinal muscularis following intestinal manipulation	239:313	Inflammation of the intestinal muscularis following intestinal manipulation may be caused by displaced bacteria or lipopolysaccharide (LPS).
34907602	7	77	theme	unique	987:992	arg1	composition					1025:1035	a unique pro-inflammatory gut microbial composition	985:1035	a unique pro-inflammatory gut microbial composition	985:1035	RESULTS We discovered that CRC patients who developed prolonged POI (PPOI) had a unique pro-inflammatory gut microbial composition during the perioperative period.
34907602	3	78	theme	underlying	523:532	arg1	mechanisms					534:543	underlying mechanisms	523:543	underlying mechanisms of LPS-triggered POI	523:564	The aim of this study was to investigate the relationship between gut microbiota, LPS, and POI in colorectal cancer (CRC) patients and explore underlying mechanisms of LPS-triggered POI.
34907602	8	79	theme	genus	1127:1131	arg1	level					1133:1137	the genus level	1123:1137	the genus level	1123:1137	The highest proportions of Gram-negative bacteria at the genus level were Escherichia-Shigella and Bacteroides; the abundance of Escherichia-Shigella was higher throughout the perioperative period.
36894427	2	0	theme	first	513:517	arg1	month					519:523	the first month	509:523	the first month of life	509:531	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	4	1	theme	DM	825:826	arg1	fat					820:822	26% fat	816:822	26% fat	816:822	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	1	theme	DM	825:826	arg1	basis					828:832	DM basis	825:832	DM basis	825:832	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	12	2	theme	liquid	1926:1931	arg1	diet					1933:1936	each liquid diet	1921:1936	each liquid diet	1921:1936	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	4	3	dep	WP	812:813	arg1	n					835:835	n = 9	835:839	n = 9	835:839	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	3	dep	WP	812:813	arg1	fat					820:822	26% fat	816:822	26% fat	816:822	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	3	dep	WP	812:813	arg1	basis					828:832	DM basis	825:832	DM basis	825:832	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	8	4	theme	jejunum	1297:1303	arg1	weights					1331:1337	jejunum and total small intestine weights	1297:1337	jejunum and total small intestine weights	1297:1337	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	1	5	theme	whole	208:212	arg1	milk					214:217	bovine whole milk	201:217	bovine whole milk	201:217	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	2	6	dep	fat	473:475	arg1	e.g.					467:470	e.g.	467:470	e.g.	467:470	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	11	7	theme	gene	1668:1671	arg1	expression					1673:1682	Tight junction protein gene expression	1645:1682	Tight junction protein gene expression in the proximal jejunum or ileum	1645:1715	Tight junction protein gene expression in the proximal jejunum or ileum did not differ between treatments.
36894427	13	8	theme	observed	2121:2128	arg1	differences					2130:2140	the observed differences	2117:2140	the observed differences	2117:2140	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	8	9	theme	total	1309:1313	arg1	intestine					1321:1329	total small intestine	1309:1329	total small intestine	1309:1329	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	9	10	theme	jejunum	1491:1497	arg1	area					1470:1473	the surface area	1458:1473	the surface area of the proximal jejunum	1458:1497	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	9	10	theme	jejunum	1491:1497	arg1	greater					1503:1509	greater	1503:1509	greater	1503:1509	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	3	11	theme	0.50	587:590	arg1	±					585:585	±	585:585	±	585:585	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	8	12	theme	intestine	1321:1329	arg1	weights					1331:1337	jejunum and total small intestine weights	1297:1337	jejunum and total small intestine weights	1297:1337	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	1	13	theme	replacer	147:154	arg1	composition					127:137	The composition	123:137	The composition of milk replacer (MR) for calves	123:170	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	11	14	theme	proximal	1691:1698	arg1	jejunum					1700:1706	the proximal jejunum	1687:1706	the proximal jejunum	1687:1706	Tight junction protein gene expression in the proximal jejunum or ileum did not differ between treatments.
36894427	3	15	from	d	592:592	arg1	arrival					604:610	arrival	604:610	arrival	604:610	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	13	16	theme	biological	2093:2102	arg1	relevance					2104:2112	the biological relevance	2089:2112	the biological relevance of the observed differences	2089:2140	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	10	17	theme	Urinary	1529:1535	arg1	recoveries					1559:1568	Urinary lactulose and Cr-EDTA recoveries	1529:1568	Urinary lactulose and Cr-EDTA recoveries	1529:1568	Urinary lactulose and Cr-EDTA recoveries were greater in calves fed WP in the first 6 h post marker administration.
36894427	12	18	theme	fatty	1899:1903	arg1	profile					1910:1916	the fatty acid profile	1895:1916	the fatty acid profile of each liquid diet	1895:1936	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	10	19	theme	Cr-EDTA	1551:1557	arg1	recoveries					1559:1568	Urinary lactulose and Cr-EDTA recoveries	1529:1568	Urinary lactulose and Cr-EDTA recoveries	1529:1568	Urinary lactulose and Cr-EDTA recoveries were greater in calves fed WP in the first 6 h post marker administration.
36894427	11	20	theme	junction	1651:1658	arg1	expression					1673:1682	Tight junction protein gene expression	1645:1682	Tight junction protein gene expression in the proximal jejunum or ileum	1645:1715	Tight junction protein gene expression in the proximal jejunum or ileum did not differ between treatments.
36894427	3	21	theme	±	618:618	arg1	deviation					629:637	mean ± standard deviation	613:637	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	13	22	theme	gastrointestinal	2015:2030	arg1	tract					2032:2036	the gastrointestinal tract	2011:2036	the gastrointestinal tract	2011:2036	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	4	23	dep	%	865:865	arg1	n					872:872	n = 9	872:876	n = 9	872:876	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	7	24	theme	WP-fed	1199:1204	arg1	calves					1206:1211	WP-fed calves	1199:1211	WP-fed calves	1199:1211	The weight of the total forestomach without contents was greater in WP-fed calves.
36894427	2	25	theme	same	439:442	arg1	profile					458:464	a same macronutrient profile	437:464	a same macronutrient profile (e.g., fat, lactose, protein)	437:494	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	4	26	theme	fat	867:869	arg1	fat					858:860	fat	858:860	fat (25% fat, n = 9)	858:877	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	26	theme	fat	867:869	arg1	%					865:865	25% fat	863:869	25% fat	863:869	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	27	theme	milk	799:802	arg1	WP					812:813	WP	812:813	WP	812:813	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	27	theme	milk	799:802	arg1	powder					804:809	a whole milk powder	791:809	a whole milk powder (WP; 26% fat, DM basis, n = 9)	791:840	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	0	28	theme	whole	73:77	arg1	powder					84:89	a whole milk powder	71:89	a whole milk powder	71:89	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	9	29	theme	surface	1374:1380	arg1	area					1382:1385	The surface area	1370:1385	The surface area of the duodenum and ileum	1370:1411	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	12	30	theme	acid	1795:1798	arg1	profiles					1800:1807	The free fatty acid and phospholipid fatty acid profiles	1752:1807	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum	1752:1841	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	2	31	dep	feeding	409:415	arg1	response					397:404	response	397:404	response	397:404	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	13	32	theme	acid	1991:1994	arg1	composition					1996:2006	fatty acid composition	1985:2006	fatty acid composition	1985:2006	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	7	33	theme	forestomach	1155:1165	arg1	weight					1135:1140	The weight	1131:1140	The weight of the total forestomach without contents	1131:1182	The weight of the total forestomach without contents was greater in WP-fed calves.
36894427	7	33	theme	forestomach	1155:1165	arg1	greater					1188:1194	greater	1188:1194	greater	1188:1194	The weight of the total forestomach without contents was greater in WP-fed calves.
36894427	12	34	theme	diet	1933:1936	arg1	profile					1910:1916	the fatty acid profile	1895:1916	the fatty acid profile of each liquid diet	1895:1936	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	13	35	theme	differences	2130:2140	arg1	relevance					2104:2112	the biological relevance	2089:2112	the biological relevance of the observed differences	2089:2140	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	4	36	theme	%	818:818	arg1	fat					820:822	26% fat	816:822	26% fat	816:822	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	36	theme	%	818:818	arg1	basis					828:832	DM basis	825:832	DM basis	825:832	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	37	theme	L	887:887	arg1	times					891:895	3.0 L 3 times	883:895	3.0 L 3 times daily (9 L total per day)	883:921	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	12	38	theme	acid	1767:1770	arg1	profiles					1800:1807	The free fatty acid and phospholipid fatty acid profiles	1752:1807	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum	1752:1841	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	2	39	theme	current	319:325	arg1	study					327:331	the current study	315:331	the current study	315:331	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	0	40	theme	Gastrointestinal	0:15	arg1	structure					17:25	Gastrointestinal structure	0:25	Gastrointestinal structure	0:25	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	3	41	dep	kg	577:578	arg1	d					592:592	1.4 ± 0.50 d	581:592	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	3	41	dep	kg	577:578	arg1	deviation					629:637	mean ± standard deviation	613:637	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	13	42	theme	gut	1964:1966	arg1	permeability					1968:1979	Feeding WP or MR altered gut permeability	1939:1979	permeability	1968:1979	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	9	43	theme	ileum	1407:1411	arg1	area					1382:1385	The surface area	1370:1385	The surface area of the duodenum and ileum	1370:1411	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	2	44	from	calves	499:504	arg1	month					519:523	the first month	509:523	the first month of life	509:531	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	12	45	theme	phospholipid	1776:1787	arg1	acid					1795:1798	phospholipid fatty acid	1776:1798	phospholipid fatty acid	1776:1798	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	2	46	theme	tract	365:369	arg1	structure					371:379	gastrointestinal tract structure	348:379	gastrointestinal tract structure	348:379	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	2	47	theme	liquid	417:422	arg1	diets					424:428	liquid diets	417:428	liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life	417:531	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	3	48	theme	male	543:546	arg1	calves					557:562	Eighteen male Holstein calves	534:562	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation)	534:638	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	12	49	theme	free	1756:1759	arg1	acid					1767:1770	free fatty acid	1756:1770	free fatty acid	1756:1770	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	2	50	contain	having	430:435	arg1	diets					424:428	liquid diets	417:428	liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life	417:531	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	2	50	contain	having	430:435	arg2	profile					458:464	a same macronutrient profile	437:464	a same macronutrient profile (e.g., fat, lactose, protein)	437:494	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	4	51	dep	age	709:711	arg1	day					725:727	day	725:727	day	725:727	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	1	52	theme	young	269:273	arg1	calves					275:280	young calves	269:280	young calves	269:280	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	13	53	theme	MR	1953:1954	arg1	permeability					1968:1979	Feeding WP or MR altered gut permeability	1939:1979	permeability	1968:1979	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	3	54	theme	5.12	572:575	arg1	±					570:570	±	570:570	±	570:570	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	3	55	theme	46.6	565:568	arg1	±					570:570	±	570:570	±	570:570	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	9	56	theme	surface	1462:1468	arg1	area					1470:1473	the surface area	1458:1473	the surface area of the proximal jejunum	1458:1497	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	9	56	theme	surface	1462:1468	arg1	greater					1503:1509	greater	1503:1509	greater	1503:1509	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	5	57	theme	indigestible	1000:1011	arg1	-EDTA					1048:1052	-EDTA	1048:1052	-EDTA	1048:1052	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	57	theme	indigestible	1000:1011	arg1	markers					1026:1032	indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol	1000:1079	indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol	1000:1079	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	57	theme	indigestible	1000:1011	arg1	[chromium					1034:1042	[chromium	1034:1042	[chromium	1034:1042	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	57	theme	indigestible	1000:1011	arg1	lactulose					1055:1063	lactulose	1055:1063	lactulose	1055:1063	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	57	theme	indigestible	1000:1011	arg1	d-mannitol					1070:1079	d-mannitol	1070:1079	d-mannitol	1070:1079	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	1	58	theme	bovine	201:206	arg1	milk					214:217	bovine whole milk	201:217	bovine whole milk	201:217	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	13	59	theme	further	2042:2048	arg1	investigation					2050:2062	further investigation	2042:2062	further investigation	2042:2062	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	8	60	theme	duodenum	1227:1234	arg1	weights					1246:1252	duodenum and ileum weights	1227:1252	duodenum and ileum weights	1227:1252	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	9	61	dep	duodenum	1394:1401	arg1	the					1390:1392	the	1390:1392	the	1390:1392	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	9	62	theme	proximal	1482:1489	arg1	jejunum					1491:1497	the proximal jejunum	1478:1497	the proximal jejunum	1478:1497	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	8	63	theme	ileum	1240:1244	arg1	weights					1246:1252	duodenum and ileum weights	1227:1252	duodenum and ileum weights	1227:1252	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	3	64	theme	age	597:599	arg1	d					592:592	1.4 ± 0.50 d	581:592	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	12	65	from	profiles	1800:1807	arg1	jejunum					1825:1831	proximal jejunum	1816:1831	proximal jejunum	1816:1831	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	12	65	from	profiles	1800:1807	arg1	ileum					1837:1841	ileum	1837:1841	ileum	1837:1841	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	1	66	theme	milk	142:145	arg1	MR					157:158	MR	157:158	MR	157:158	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	1	66	theme	milk	142:145	arg1	replacer					147:154	milk replacer	142:154	milk replacer (MR)	142:159	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	0	67	theme	dairy	54:58	arg1	calves					60:65	preweaning dairy calves	43:65	preweaning dairy calves	43:65	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	2	68	theme	life	528:531	arg1	month					519:523	the first month	509:523	the first month of life	509:531	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	8	69	theme	small	1315:1319	arg1	intestine					1321:1329	total small intestine	1309:1329	total small intestine	1309:1329	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	3	70	theme	mean	613:616	arg1	deviation					629:637	mean ± standard deviation	613:637	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	0	71	theme	high	110:113	arg1	replacer					101:108	a milk replacer	94:108	a milk replacer high in fat	94:120	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	4	72	dep	fed	879:881	arg1	high					850:853	high	850:853	high	850:853	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	12	73	theme	acid	1905:1908	arg1	profile					1910:1916	the fatty acid profile	1895:1916	the fatty acid profile of each liquid diet	1895:1936	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	11	74	from	expression	1673:1682	arg1	jejunum					1700:1706	the proximal jejunum	1687:1706	the proximal jejunum	1687:1706	Tight junction protein gene expression in the proximal jejunum or ileum did not differ between treatments.
36894427	11	74	from	expression	1673:1682	arg1	ileum					1711:1715	ileum	1711:1715	ileum	1711:1715	Tight junction protein gene expression in the proximal jejunum or ileum did not differ between treatments.
36894427	10	75	theme	lactulose	1537:1545	arg1	recoveries					1559:1568	Urinary lactulose and Cr-EDTA recoveries	1529:1568	Urinary lactulose and Cr-EDTA recoveries	1529:1568	Urinary lactulose and Cr-EDTA recoveries were greater in calves fed WP in the first 6 h post marker administration.
36894427	8	76	theme	WP-fed	1355:1360	arg1	calves					1362:1367	WP-fed calves	1355:1367	WP-fed calves	1355:1367	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	11	77	theme	protein	1660:1666	arg1	expression					1673:1682	Tight junction protein gene expression	1645:1682	Tight junction protein gene expression in the proximal jejunum or ileum	1645:1715	Tight junction protein gene expression in the proximal jejunum or ileum did not differ between treatments.
36894427	3	78	theme	standard	620:627	arg1	deviation					629:637	mean ± standard deviation	613:637	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	13	79	theme	tract	2032:2036	arg1	permeability					1968:1979	Feeding WP or MR altered gut permeability	1939:1979	permeability	1968:1979	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	13	79	theme	tract	2032:2036	arg1	WP					1947:1948	Feeding WP or MR altered gut permeability	1939:1979	WP	1947:1948	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	13	79	theme	tract	2032:2036	arg1	composition					1996:2006	fatty acid composition	1985:2006	fatty acid composition	1985:2006	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	13	79	theme	tract	2032:2036	arg1	investigation					2050:2062	further investigation	2042:2062	further investigation	2042:2062	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	11	80	theme	Tight	1645:1649	arg1	expression					1673:1682	Tight junction protein gene expression	1645:1682	Tight junction protein gene expression in the proximal jejunum or ileum	1645:1715	Tight junction protein gene expression in the proximal jejunum or ileum did not differ between treatments.
36894427	4	81	theme	=	837:837	arg1	n					835:835	n = 9	835:839	n = 9	835:839	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	82	theme	=	874:874	arg1	n					872:872	n = 9	872:876	n = 9	872:876	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	0	83	theme	milk	79:82	arg1	powder					84:89	a whole milk powder	71:89	a whole milk powder	71:89	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	4	84	theme	whole	793:797	arg1	WP					812:813	WP	812:813	WP	812:813	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	84	theme	whole	793:797	arg1	powder					804:809	a whole milk powder	791:809	a whole milk powder (WP; 26% fat, DM basis, n = 9)	791:840	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	10	85	theme	first	1607:1611	arg1	h					1615:1615	the first 6 h	1603:1615	the first 6 h post marker administration	1603:1642	Urinary lactulose and Cr-EDTA recoveries were greater in calves fed WP in the first 6 h post marker administration.
36894427	13	86	theme	fatty	1985:1989	arg1	composition					1996:2006	fatty acid composition	1985:2006	fatty acid composition	1985:2006	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	7	87	theme	total	1149:1153	arg1	forestomach					1155:1165	the total forestomach	1145:1165	the total forestomach without contents	1145:1182	The weight of the total forestomach without contents was greater in WP-fed calves.
36894427	12	88	theme	proximal	1816:1823	arg1	jejunum					1825:1831	proximal jejunum	1816:1831	proximal jejunum	1816:1831	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	4	89	theme	total	908:912	arg1	L					906:906	9 L total	904:912	9 L total	904:912	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	12	90	theme	fatty	1789:1793	arg1	acid					1795:1798	phospholipid fatty acid	1776:1798	phospholipid fatty acid	1776:1798	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	0	91	theme	milk	96:99	arg1	replacer					101:108	a milk replacer	94:108	a milk replacer high in fat	94:120	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	2	92	theme	macronutrient	444:456	arg1	profile					458:464	a same macronutrient profile	437:464	a same macronutrient profile (e.g., fat, lactose, protein)	437:494	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	3	93	from	arrival	604:610	arg1	d					592:592	1.4 ± 0.50 d	581:592	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	3	93	from	arrival	604:610	arg1	age					597:599	age	597:599	age at arrival	597:610	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	5	94	theme	gut	965:967	arg1	permeability					969:980	gut permeability	965:980	gut permeability	965:980	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	2	95	theme	study	327:331	arg1	objective					302:310	the objective	298:310	the objective of the current study	298:331	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	13	96	theme	altered	1956:1962	arg1	permeability					1968:1979	Feeding WP or MR altered gut permeability	1939:1979	permeability	1968:1979	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	5	97	dep	markers	1026:1032	arg1	-EDTA					1048:1052	-EDTA	1048:1052	-EDTA	1048:1052	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	97	dep	markers	1026:1032	arg1	markers					1026:1032	indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol	1000:1079	indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol	1000:1079	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	97	dep	markers	1026:1032	arg1	[chromium					1034:1042	[chromium	1034:1042	[chromium	1034:1042	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	97	dep	markers	1026:1032	arg1	lactulose					1055:1063	lactulose	1055:1063	lactulose	1055:1063	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	97	dep	markers	1026:1032	arg1	d-mannitol					1070:1079	d-mannitol	1070:1079	d-mannitol	1070:1079	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	97	dep	markers	1026:1032	arg1	Cr					1045:1046	Cr	1045:1046	Cr	1045:1046	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	4	98	theme	26	816:817	arg1	%					818:818	%	818:818	%	818:818	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	4	99	theme	daily	897:901	arg1	times					891:895	3.0 L 3 times	883:895	3.0 L 3 times daily (9 L total per day)	883:921	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	12	100	theme	fatty	1761:1765	arg1	acid					1767:1770	free fatty acid	1756:1770	free fatty acid	1756:1770	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	12	101	dep	jejunum	1825:1831	arg1	the					1812:1814	the	1812:1814	the	1812:1814	The free fatty acid and phospholipid fatty acid profiles in the proximal jejunum and ileum differed between treatments and generally reflected the fatty acid profile of each liquid diet.
36894427	2	102	dep	profile	458:464	arg1	protein					487:493	protein	487:493	protein	487:493	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	2	102	dep	profile	458:464	arg1	lactose					478:484	lactose	478:484	lactose	478:484	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	2	102	dep	profile	458:464	arg1	fat					473:475	fat	473:475	fat	473:475	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	3	103	theme	Holstein	548:555	arg1	calves					557:562	Eighteen male Holstein calves	534:562	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation)	534:638	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	2	104	theme	gastrointestinal	348:363	arg1	structure					371:379	gastrointestinal tract structure	348:379	gastrointestinal tract structure	348:379	In this light, the objective of the current study was to compare gastrointestinal tract structure and function in response to feeding liquid diets having a same macronutrient profile (e.g., fat, lactose, protein) in calves in the first month of life.
36894427	0	105	theme	preweaning	43:52	arg1	calves					60:65	preweaning dairy calves	43:65	preweaning dairy calves	43:65	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	1	106	theme	gastrointestinal	237:252	arg1	development					254:264	gastrointestinal development	237:264	gastrointestinal development of young calves	237:280	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	13	107	theme	Feeding	1939:1945	arg1	WP					1947:1948	Feeding WP or MR altered gut permeability	1939:1979	WP	1947:1948	Feeding WP or MR altered gut permeability and fatty acid composition of the gastrointestinal tract and further investigation are needed to understand the biological relevance of the observed differences.
36894427	4	108	theme	teat	942:945	arg1	buckets					947:953	teat buckets	942:953	teat buckets	942:953	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	5	109	theme	permeability	1013:1024	arg1	-EDTA					1048:1052	-EDTA	1048:1052	-EDTA	1048:1052	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	109	theme	permeability	1013:1024	arg1	markers					1026:1032	indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol	1000:1079	indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol	1000:1079	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	109	theme	permeability	1013:1024	arg1	[chromium					1034:1042	[chromium	1034:1042	[chromium	1034:1042	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	109	theme	permeability	1013:1024	arg1	lactulose					1055:1063	lactulose	1055:1063	lactulose	1055:1063	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	5	109	theme	permeability	1013:1024	arg1	d-mannitol					1070:1079	d-mannitol	1070:1079	d-mannitol	1070:1079	On d 21, gut permeability was assessed with indigestible permeability markers [chromium (Cr)-EDTA, lactulose, and d-mannitol].
36894427	0	110	theme	calves	60:65	arg1	function					31:38	function	31:38	function	31:38	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	0	110	theme	calves	60:65	arg1	structure					17:25	Gastrointestinal structure	0:25	Gastrointestinal structure	0:25	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	9	111	theme	treatment	1436:1444	arg1	groups					1446:1451	treatment groups	1436:1451	treatment groups	1436:1451	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	4	112	dep	daily	897:901	arg1	L					906:906	9 L total	904:912	9 L total	904:912	Upon arrival, calves were blocked based on age and arrival day, and, within a block, calves were randomly assigned to either a whole milk powder (WP; 26% fat, DM basis, n = 9) or a MR high in fat (25% fat, n = 9) fed 3.0 L 3 times daily (9 L total per day) at 135 g/L through teat buckets.
36894427	1	113	theme	calves	275:280	arg1	development					254:264	gastrointestinal development	237:264	gastrointestinal development of young calves	237:280	The composition of milk replacer (MR) for calves greatly differs from that of bovine whole milk, which may affect gastrointestinal development of young calves.
36894427	10	114	theme	marker	1622:1627	arg1	administration					1629:1642	marker administration	1622:1642	marker administration	1622:1642	Urinary lactulose and Cr-EDTA recoveries were greater in calves fed WP in the first 6 h post marker administration.
36894427	0	115	from	fat	118:120	arg1	high					110:113	high	110:113	high	110:113	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	8	116	theme	treatment	1275:1283	arg1	groups					1285:1290	treatment groups	1275:1290	treatment groups	1275:1290	Furthermore, duodenum and ileum weights were similar between treatment groups, but jejunum and total small intestine weights were greater in WP-fed calves.
36894427	0	117	from	high	110:113	arg1	fat					118:120	fat	118:120	fat	118:120	Gastrointestinal structure and function of preweaning dairy calves fed a whole milk powder or a milk replacer high in fat.
36894427	3	118	theme	1.4	581:583	arg1	±					585:585	±	585:585	±	585:585	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36894427	9	119	theme	duodenum	1394:1401	arg1	area					1382:1385	The surface area	1370:1385	The surface area of the duodenum and ileum	1370:1411	The surface area of the duodenum and ileum did not differ between treatment groups, but the surface area of the proximal jejunum was greater in calves fed WP.
36894427	3	120	dep	calves	557:562	arg1	kg					577:578	46.6 ± 5.12 kg	565:578	46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation	565:637	Eighteen male Holstein calves (46.6 ± 5.12 kg; 1.4 ± 0.50 d of age at arrival; mean ± standard deviation) were housed individually.
36934842	7	0	theme	ATGL	1369:1372	arg1	atglistatin					1385:1395	atglistatin	1385:1395	atglistatin	1385:1395	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	7	0	theme	ATGL	1369:1372	arg1	inhibitor					1374:1382	ATGL inhibitor	1369:1382	ATGL inhibitor	1369:1382	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	8	1	theme	PGI2	1732:1735	arg1	generation					1737:1746	ATGL-dependent PGI2 generation	1717:1746	ATGL-dependent PGI2 generation	1717:1746	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	8	2	theme	unsaturated	1644:1654	arg1	lipids					1656:1661	highly unsaturated or less unsaturated lipids	1617:1661	highly unsaturated or less unsaturated lipids	1617:1661	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	1	3	theme	droplets	139:146	arg1	Biogenesis					119:128	Biogenesis	119:128	Biogenesis of lipid droplets (LDs) in various cells	119:169	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	6	4	contain	had	1173:1175	arg1	LDs					1122:1124	these LDs	1116:1124	these LDs	1116:1124	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	6	4	contain	had	1173:1175	arg2	content					1190:1196	a negligible content	1177:1196	a negligible content of phospholipids and cholesterols	1177:1230	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	6	5	theme	phospholipids	1201:1213	arg1	content					1190:1196	a negligible content	1177:1196	a negligible content of phospholipids and cholesterols	1177:1230	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	4	6	theme	fluorescence	759:770	arg1	imaging					772:778	fluorescence imaging	759:778	fluorescence imaging	759:778	The abundance, size, and biochemical composition of LDs was characterized based on Raman spectroscopy and fluorescence imaging.
36934842	4	7	dep	abundance	657:665	arg1	The					653:655	The	653:655	The	653:655	The abundance, size, and biochemical composition of LDs was characterized based on Raman spectroscopy and fluorescence imaging.
36934842	1	8	theme	pathological	224:235	arg1	processes					237:245	various physiological and pathological processes	198:245	various physiological and pathological processes	198:245	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	0	9	theme	biochemical	66:76	arg1	composition					78:88	biochemical composition	66:88	biochemical composition	66:88	Vascular lipid droplets formed in response to TNF, hypoxia or OA: biochemical composition and prostacyclin generation.
36934842	3	10	theme	hypoxic	613:619	arg1	conditions					621:630	hypoxic conditions	613:630	hypoxic conditions	613:630	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	9	11	theme	vascular	1856:1863	arg1	insult					1882:1887	vascular pro-inflammatory insult	1856:1887	vascular pro-inflammatory insult	1856:1887	In conclusion, vascular LDs formation and ATGL-dependent PGI2 generation represent a universal response to vascular pro-inflammatory insult.
36934842	9	12	theme	vascular	1764:1771	arg1	formation					1777:1785	vascular LDs formation	1764:1785	vascular LDs formation	1764:1785	In conclusion, vascular LDs formation and ATGL-dependent PGI2 generation represent a universal response to vascular pro-inflammatory insult.
36934842	6	13	from	rich	1135:1138	arg1	lipids					1162:1167	highly unsaturated lipids	1143:1167	highly unsaturated lipids	1143:1167	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	5	14	theme	triglyceride	832:843	arg1	ATGL					853:856	ATGL	853:856	ATGL	853:856	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	5	14	theme	triglyceride	832:843	arg1	lipase					845:850	the adipose triglyceride lipase	820:850	the adipose triglyceride lipase (ATGL)	820:857	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	8	15	dep	independent	1487:1497	arg1	induced					1519:1525	induced	1519:1525	were induced by pro-inflammatory stimuli, hypoxia, or oleic acid	1514:1577	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	8	15	dep	independent	1487:1497	arg1	composed					1605:1612	composed	1605:1612	were composed of highly unsaturated or less unsaturated lipids	1600:1661	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	7	16	theme	OA-overload	1316:1326	arg1	conditions					1328:1337	hypoxic or OA-overload conditions	1305:1337	hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin	1305:1395	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	8	17	theme	LDs	1676:1678	arg1	formation					1680:1688	LDs formation	1676:1688	LDs formation	1676:1688	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	0	18	dep	composition	78:88	arg1	droplets					15:22	Vascular lipid droplets	0:22	Vascular lipid droplets formed in response to TNF, hypoxia or OA	0:63	Vascular lipid droplets formed in response to TNF, hypoxia or OA: biochemical composition and prostacyclin generation.
36934842	9	19	theme	LDs	1773:1775	arg1	formation					1777:1785	vascular LDs formation	1764:1785	vascular LDs formation	1764:1785	In conclusion, vascular LDs formation and ATGL-dependent PGI2 generation represent a universal response to vascular pro-inflammatory insult.
36934842	5	20	theme	adipose	824:830	arg1	ATGL					853:856	ATGL	853:856	ATGL	853:856	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	5	20	theme	adipose	824:830	arg1	lipase					845:850	the adipose triglyceride lipase	820:850	the adipose triglyceride lipase (ATGL)	820:857	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	4	21	theme	Raman	736:740	arg1	spectroscopy					742:753	Raman spectroscopy	736:753	Raman spectroscopy	736:753	The abundance, size, and biochemical composition of LDs was characterized based on Raman spectroscopy and fluorescence imaging.
36934842	3	22	theme	aortas	476:481	arg1	tissue					450:455	the vascular tissue	437:455	the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA)	437:650	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	8	23	theme	oleic	1568:1572	arg1	stimuli					1547:1553	pro-inflammatory stimuli	1530:1553	pro-inflammatory stimuli	1530:1553	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	8	23	theme	oleic	1568:1572	arg1	acid					1574:1577	oleic acid	1568:1577	oleic acid	1568:1577	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	3	24	theme	LDs	395:397	arg1	generation					423:432	LDs and prostacyclin (PGI2) generation	395:432	LDs and prostacyclin (PGI2) generation	395:432	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	6	25	theme	cholesterols	1219:1230	arg1	content					1190:1196	a negligible content	1177:1196	a negligible content of phospholipids and cholesterols	1177:1230	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	7	26	theme	hypoxic	1305:1311	arg1	conditions					1328:1337	hypoxic or OA-overload conditions	1305:1337	hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin	1305:1395	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	7	27	theme	endothelial	1283:1293	arg1	LDs					1295:1297	endothelial LDs	1283:1297	endothelial LDs	1283:1297	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	0	28	theme	lipid	9:13	arg1	droplets					15:22	Vascular lipid droplets	0:22	Vascular lipid droplets formed in response to TNF, hypoxia or OA	0:63	Vascular lipid droplets formed in response to TNF, hypoxia or OA: biochemical composition and prostacyclin generation.
36934842	3	29	theme	oleic	636:640	arg1	OA					648:649	OA	648:649	OA	648:649	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	3	29	theme	oleic	636:640	arg1	acid					642:645	oleic acid	636:645	oleic acid (OA)	636:650	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	1	30	from	Biogenesis	119:128	arg1	cells					165:169	various cells	157:169	various cells	157:169	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	2	31	theme	endothelial	280:290	arg1	physiology					292:301	endothelial physiology	280:301	endothelial physiology	280:301	However, the function of LDs in endothelial physiology and pathology is not well understood.
36934842	9	32	theme	ATGL-dependent	1791:1804	arg1	generation					1811:1820	ATGL-dependent PGI2 generation	1791:1820	ATGL-dependent PGI2 generation	1791:1820	In conclusion, vascular LDs formation and ATGL-dependent PGI2 generation represent a universal response to vascular pro-inflammatory insult.
36934842	7	33	theme	biochemical	1443:1453	arg1	composition					1455:1465	the biochemical composition	1439:1465	the biochemical composition of LDs	1439:1472	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	0	34	theme	Vascular	0:7	arg1	droplets					15:22	Vascular lipid droplets	0:22	Vascular lipid droplets formed in response to TNF, hypoxia or OA	0:63	Vascular lipid droplets formed in response to TNF, hypoxia or OA: biochemical composition and prostacyclin generation.
36934842	5	35	theme	pro-inflammatory	959:974	arg1	stimuli					976:982	all tested pro-inflammatory stimuli	948:982	all tested pro-inflammatory stimuli	948:982	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	3	36	theme	tumor	533:537	arg1	TNF					556:558	TNF	556:558	TNF	556:558	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	3	36	theme	tumor	533:537	arg1	factor					548:553	tumor necrosis factor	533:553	tumor necrosis factor (TNF)	533:559	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	9	37	theme	PGI2	1806:1809	arg1	generation					1811:1820	ATGL-dependent PGI2 generation	1791:1820	ATGL-dependent PGI2 generation	1791:1820	In conclusion, vascular LDs formation and ATGL-dependent PGI2 generation represent a universal response to vascular pro-inflammatory insult.
36934842	1	38	theme	various	157:163	arg1	cells					165:169	various cells	157:169	various cells	157:169	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	7	39	theme	LDs	1295:1297	arg1	signature					1270:1278	the Raman signature	1260:1278	the Raman signature of endothelial LDs	1260:1297	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	2	40	theme	LDs	273:275	arg1	function					261:268	the function	257:268	the function of LDs in endothelial physiology and pathology	257:315	However, the function of LDs in endothelial physiology and pathology is not well understood.
36934842	8	41	dep	observed	1667:1674	arg1	associated					1701:1710	associated	1701:1710	associated with ATGL-dependent PGI2 generation	1701:1746	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	6	42	theme	spectra	1020:1026	arg1	analysis					1002:1009	the analysis	998:1009	the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia	998:1099	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	0	43	theme	prostacyclin	94:105	arg1	generation					107:116	prostacyclin generation	94:116	prostacyclin generation	94:116	Vascular lipid droplets formed in response to TNF, hypoxia or OA: biochemical composition and prostacyclin generation.
36934842	6	44	theme	Raman	1014:1018	arg1	spectra					1020:1026	Raman spectra	1014:1026	Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia	1014:1099	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	7	45	dep	presence	1346:1353	arg1	the					1342:1344	the	1342:1344	the	1342:1344	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	6	46	theme	LDs	1031:1033	arg1	spectra					1020:1026	Raman spectra	1014:1026	Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia	1014:1099	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	6	47	from	vessels	1051:1057	arg1	analysis					1002:1009	the analysis	998:1009	the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia	998:1099	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	3	48	theme	prostacyclin	403:414	arg1	generation					423:432	LDs and prostacyclin (PGI2) generation	395:432	LDs and prostacyclin (PGI2) generation	395:432	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	4	49	theme	LDs	705:707	arg1	composition					690:700	biochemical composition	678:700	biochemical composition	678:700	The abundance, size, and biochemical composition of LDs was characterized based on Raman spectroscopy and fluorescence imaging.
36934842	4	49	theme	LDs	705:707	arg1	size					668:671	size	668:671	size	668:671	The abundance, size, and biochemical composition of LDs was characterized based on Raman spectroscopy and fluorescence imaging.
36934842	4	49	theme	LDs	705:707	arg1	abundance					657:665	abundance	657:665	abundance	657:665	The abundance, size, and biochemical composition of LDs was characterized based on Raman spectroscopy and fluorescence imaging.
36934842	9	50	theme	universal	1834:1842	arg1	response					1844:1851	a universal response	1832:1851	a universal response to vascular pro-inflammatory insult	1832:1887	In conclusion, vascular LDs formation and ATGL-dependent PGI2 generation represent a universal response to vascular pro-inflammatory insult.
36934842	1	51	theme	important	180:188	arg1	role					190:193	an important role	177:193	an important role	177:193	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	9	52	theme	pro-inflammatory	1865:1880	arg1	insult					1882:1887	vascular pro-inflammatory insult	1856:1887	vascular pro-inflammatory insult	1856:1887	In conclusion, vascular LDs formation and ATGL-dependent PGI2 generation represent a universal response to vascular pro-inflammatory insult.
36934842	6	53	theme	negligible	1179:1188	arg1	content					1190:1196	a negligible content	1177:1196	a negligible content of phospholipids and cholesterols	1177:1230	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	3	54	theme	necrosis	539:546	arg1	TNF					556:558	TNF	556:558	TNF	556:558	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	3	54	theme	necrosis	539:546	arg1	factor					548:553	tumor necrosis factor	533:553	tumor necrosis factor (TNF)	533:559	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	6	55	from	spectra	1020:1026	arg1	vessels					1051:1057	the isolated vessels	1038:1057	the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia	1038:1099	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	5	56	theme	lipolysis	807:815	arg1	blockade					795:802	blockade	795:802	blockade of lipolysis by the adipose triglyceride lipase (ATGL)	795:857	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	8	57	theme	ATGL-dependent	1717:1730	arg1	generation					1737:1746	ATGL-dependent PGI2 generation	1717:1746	ATGL-dependent PGI2 generation	1717:1746	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	6	58	from	lipids	1162:1167	arg1	rich					1135:1138	rich	1135:1138	rich	1135:1138	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	6	59	theme	isolated	1042:1049	arg1	vessels					1051:1057	the isolated vessels	1038:1057	the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia	1038:1099	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	8	60	theme	unsaturated	1624:1634	arg1	lipids					1656:1661	highly unsaturated or less unsaturated lipids	1617:1661	highly unsaturated or less unsaturated lipids	1617:1661	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	3	61	theme	vascular	441:448	arg1	tissue					450:455	the vascular tissue	437:455	the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA)	437:650	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	7	62	from	conditions	1328:1337	arg1	absence					1358:1364	absence	1358:1364	absence	1358:1364	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	7	62	from	conditions	1328:1337	arg1	presence					1346:1353	presence	1346:1353	presence	1346:1353	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	6	63	theme	unsaturated	1150:1160	arg1	lipids					1162:1167	highly unsaturated lipids	1143:1167	highly unsaturated lipids	1143:1167	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	7	64	theme	inhibitor	1374:1382	arg1	absence					1358:1364	absence	1358:1364	absence	1358:1364	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	7	64	theme	inhibitor	1374:1382	arg1	presence					1346:1353	presence	1346:1353	presence	1346:1353	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	4	65	theme	biochemical	678:688	arg1	composition					690:700	biochemical composition	678:700	biochemical composition	678:700	The abundance, size, and biochemical composition of LDs was characterized based on Raman spectroscopy and fluorescence imaging.
36934842	7	66	theme	Raman	1264:1268	arg1	signature					1270:1278	the Raman signature	1260:1278	the Raman signature of endothelial LDs	1260:1297	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	3	67	theme	generation	423:432	arg1	formation					382:390	the formation	378:390	the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA)	378:650	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	3	68	theme	pro-inflammatory	507:522	arg1	factors					524:530	pro-inflammatory factors	507:530	pro-inflammatory factors	507:530	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	8	69	theme	pro-inflammatory	1530:1545	arg1	hypoxia					1556:1562	hypoxia	1556:1562	hypoxia	1556:1562	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	8	69	theme	pro-inflammatory	1530:1545	arg1	stimuli					1547:1553	pro-inflammatory stimuli	1530:1553	pro-inflammatory stimuli	1530:1553	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	8	69	theme	pro-inflammatory	1530:1545	arg1	acid					1574:1577	oleic acid	1568:1577	oleic acid	1568:1577	Altogether, independent of whether LDs were induced by pro-inflammatory stimuli, hypoxia, or oleic acid, and of whether they were composed of highly unsaturated or less unsaturated lipids, we observed LDs formation invariably associated with ATGL-dependent PGI2 generation.
36934842	5	70	theme	tested	952:957	arg1	stimuli					976:982	all tested pro-inflammatory stimuli	948:982	all tested pro-inflammatory stimuli	948:982	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	3	71	from	formation	382:390	arg1	tissue					450:455	the vascular tissue	437:455	the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA)	437:650	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	3	72	theme	murine	469:474	arg1	aortas					476:481	isolated murine aortas	460:481	isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA)	460:650	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	2	73	from	function	261:268	arg1	pathology					307:315	pathology	307:315	pathology	307:315	However, the function of LDs in endothelial physiology and pathology is not well understood.
36934842	2	73	from	function	261:268	arg1	physiology					292:301	endothelial physiology	280:301	endothelial physiology	280:301	However, the function of LDs in endothelial physiology and pathology is not well understood.
36934842	7	74	theme	LDs	1470:1472	arg1	composition					1455:1465	the biochemical composition	1439:1465	the biochemical composition of LDs	1439:1472	Additionally, by comparing the Raman signature of endothelial LDs under hypoxic or OA-overload conditions in the presence or absence of ATGL inhibitor, atglistatin, we show that atglistatin does not affect the biochemical composition of LDs.
36934842	5	75	theme	LDs	867:869	arg1	degradation					871:881	LDs degradation	867:881	LDs degradation	867:881	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	3	76	theme	isolated	460:467	arg1	aortas					476:481	isolated murine aortas	460:481	isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA)	460:650	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	3	77	theme	present	348:354	arg1	work					356:359	the present work	344:359	the present work	344:359	In the present work, we investigated the formation of LDs and prostacyclin (PGI2) generation in the vascular tissue of isolated murine aortas following activation by pro-inflammatory factors: tumor necrosis factor (TNF), lipopolysaccharides (LPS), angiotensin II (AngII), hypoxic conditions, or oleic acid (OA).
36934842	1	78	theme	various	198:204	arg1	processes					237:245	various physiological and pathological processes	198:245	various physiological and pathological processes	198:245	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	6	79	from	analysis	1002:1009	arg1	vessels					1051:1057	the isolated vessels	1038:1057	the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia	1038:1099	Furthermore, the analysis of Raman spectra of LDs in the isolated vessels stimulated by TNF, LPS, AngII, or hypoxia uncovered that these LDs were all rich in highly unsaturated lipids and had a negligible content of phospholipids and cholesterols.
36934842	5	80	theme	PGI2	910:913	arg1	generation					915:924	PGI2 generation	910:924	PGI2 generation	910:924	We found that blockade of lipolysis by the adipose triglyceride lipase (ATGL) delayed LDs degradation and simultaneously blunted PGI2 generation in aorta treated with all tested pro-inflammatory stimuli.
36934842	1	81	theme	lipid	133:137	arg1	LDs					149:151	LDs	149:151	LDs	149:151	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	1	81	theme	lipid	133:137	arg1	droplets					139:146	lipid droplets	133:146	lipid droplets (LDs)	133:152	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36934842	1	82	theme	physiological	206:218	arg1	processes					237:245	various physiological and pathological processes	198:245	various physiological and pathological processes	198:245	Biogenesis of lipid droplets (LDs) in various cells plays an important role in various physiological and pathological processes.
36557882	0	0	theme	Bone	82:85	arg1	Regeneration					87:98	Bone Regeneration	82:98	Bone Regeneration	82:98	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.
36557882	2	1	theme	properties	468:477	arg1	analyses					441:448	analyses	441:448	analyses of its biological properties using human mesenchymal stem cells (MSCs)	441:519	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	7	2	theme	osteogenic	1391:1400	arg1	differentiation					1402:1416	osteogenic differentiation	1391:1416	osteogenic differentiation	1391:1416	In vitro results showed that the MeHA-HAp coating promotes MSCs adhesion and proliferation and contributes to osteogenic differentiation and extracellular matrix mineralization.
36557882	1	3	theme	printed	140:146	arg1	scaffold					176:183	a 3D printed polyetheretherketone (PEEK) scaffold	135:183	a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	135:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	1	4	theme	-hydroxyapatite	233:247	arg1	hydrogel					255:262	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	2	5	theme	biological	457:466	arg1	properties					468:477	its biological properties	453:477	its biological properties using human mesenchymal stem cells (MSCs)	453:519	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	7	6	theme	extracellular	1422:1434	arg1	mineralization					1443:1456	extracellular matrix mineralization	1422:1456	extracellular matrix mineralization	1422:1456	In vitro results showed that the MeHA-HAp coating promotes MSCs adhesion and proliferation and contributes to osteogenic differentiation and extracellular matrix mineralization.
36557882	6	7	dep	28	1189:1190	arg1	to					1186:1187	to	1186:1187	to	1186:1187	Human MSCs were seeded on bare and coated scaffolds and cultured for up to 28 days to determine the adhesion, proliferation, migration and osteogenic differentiation.
36557882	1	8	theme	polyetheretherketone	148:167	arg1	scaffold					176:183	a 3D printed polyetheretherketone (PEEK) scaffold	135:183	a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	135:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	5	9	theme	Young	1002:1006	arg1	modulus					1010:1016	Young's modulus	1002:1016	Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively	1002:1111	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	7	10	theme	matrix	1436:1441	arg1	mineralization					1443:1456	extracellular matrix mineralization	1422:1456	extracellular matrix mineralization	1422:1456	In vitro results showed that the MeHA-HAp coating promotes MSCs adhesion and proliferation and contributes to osteogenic differentiation and extracellular matrix mineralization.
36557882	8	11	theme	clinical	1674:1681	arg1	applications					1683:1694	translational clinical applications	1660:1694	translational clinical applications	1660:1694	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	5	12	theme	lattice	916:922	arg1	geometry					924:931	the lattice geometry	912:931	the lattice geometry	912:931	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	1	13	theme	HAp	250:252	arg1	hydrogel					255:262	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	5	14	theme	MeHA-HAp	898:905	arg1	inclusion					885:893	the inclusion	881:893	the inclusion of MeHA-HAp into the lattice geometry	881:931	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	3	15	theme	Interconnected	522:535	arg1	matrices					549:556	Interconnected porous PEEK matrices	522:556	Interconnected porous PEEK matrices	522:556	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	3	16	with	pattern	636:642	arg1	orientation					663:673	0°/90° raster orientation	649:673	0°/90° raster orientation	649:673	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	3	16	with	pattern	636:642	arg1	pores					686:690	square pores	679:690	square pores	679:690	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	5	17	theme	coated	817:822	arg1	scaffold					838:845	the coated and pure PEEK scaffold	813:845	the coated and pure PEEK scaffold	813:845	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	2	18	theme	stem	503:506	arg1	MSCs					515:518	MSCs	515:518	MSCs	515:518	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	2	18	theme	stem	503:506	arg1	cells					508:512	human mesenchymal stem cells	485:512	human mesenchymal stem cells (MSCs)	485:519	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	5	19	theme	1020.0	1044:1049	arg1	±					1051:1051	±	1051:1051	±	1051:1051	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	7	20	theme	MeHA-HAp	1314:1321	arg1	coating					1323:1329	the MeHA-HAp coating	1310:1329	the MeHA-HAp coating	1310:1329	In vitro results showed that the MeHA-HAp coating promotes MSCs adhesion and proliferation and contributes to osteogenic differentiation and extracellular matrix mineralization.
36557882	7	21	dep	In	1281:1282	arg1	vitro					1284:1288	vitro	1284:1288	vitro	1284:1288	In vitro results showed that the MeHA-HAp coating promotes MSCs adhesion and proliferation and contributes to osteogenic differentiation and extracellular matrix mineralization.
36557882	1	22	dep	substitute	113:122	arg1	made					124:127	made	124:127	substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	113:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	8	23	theme	translational	1660:1672	arg1	applications					1683:1694	translational clinical applications	1660:1694	translational clinical applications	1660:1694	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	5	24	theme	pure	828:831	arg1	scaffold					838:845	the coated and pure PEEK scaffold	813:845	the coated and pure PEEK scaffold	813:845	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	2	25	theme	mesenchymal	491:501	arg1	MSCs					515:518	MSCs	515:518	MSCs	515:518	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	2	25	theme	mesenchymal	491:501	arg1	cells					508:512	human mesenchymal stem cells	485:512	human mesenchymal stem cells (MSCs)	485:519	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	8	26	theme	bioactive	1596:1604	arg1	properties					1606:1615	the bioactive properties	1592:1615	the bioactive properties of MeHA and HAp	1592:1631	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	1	27	theme	PEEK	170:173	arg1	scaffold					176:183	a 3D printed polyetheretherketone (PEEK) scaffold	135:183	a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	135:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	5	28	theme	63.7	1053:1056	arg1	±					1051:1051	±	1051:1051	±	1051:1051	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	8	29	theme	mechanical	1555:1564	arg1	performances					1566:1577	the great mechanical performances	1545:1577	the great mechanical performances of PEEK	1545:1585	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	4	30	theme	MeHA-HAp	708:715	arg1	slurry					717:722	a MeHA-HAp slurry	706:722	a MeHA-HAp slurry	706:722	In parallel, a MeHA-HAp slurry has been synthesized and infiltrated in the PEEK scaffolds.
36557882	8	31	theme	efficient	1482:1490	arg1	solution					1492:1499	an efficient solution	1479:1499	an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp	1479:1631	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	5	32	theme	scaffold	838:845	arg1	properties					799:808	The mechanical properties	784:808	The mechanical properties of the coated and pure PEEK scaffold	784:845	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	0	33	theme	PEEK	0:3	arg1	Structures					37:46	PEEK and Hyaluronan-Based 3D Printed Structures	0:46	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.	0:99	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.
36557882	5	34	theme	PEEK	833:836	arg1	scaffold					838:845	the coated and pure PEEK scaffold	813:845	the coated and pure PEEK scaffold	813:845	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	5	35	theme	±	1028:1028	arg1	MPa					1036:1038	1034.9 ± 126.1 MPa	1021:1038	1034.9 ± 126.1 MPa	1021:1038	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	0	36	theme	3D	26:27	arg1	Structures					37:46	PEEK and Hyaluronan-Based 3D Printed Structures	0:46	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.	0:99	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.
36557882	7	37	theme	In	1281:1282	arg1	results					1290:1296	In vitro results	1281:1296	In vitro results	1281:1296	In vitro results showed that the MeHA-HAp coating promotes MSCs adhesion and proliferation and contributes to osteogenic differentiation and extracellular matrix mineralization.
36557882	1	38	theme	Hybrid	101:106	arg1	objective					271:279	the objective	267:279	the objective of the present work	267:299	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	1	38	theme	Hybrid	101:106	arg1	bone					108:111	Hybrid bone	101:111	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	101:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	2	39	theme	UV	398:399	arg1	photocrosslinking					401:417	UV photocrosslinking	398:417	UV photocrosslinking	398:417	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	0	40	theme	Hyaluronan-Based	9:24	arg1	Structures					37:46	PEEK and Hyaluronan-Based 3D Printed Structures	0:46	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.	0:99	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.
36557882	8	41	theme	scaffold	1526:1533	arg1	development					1509:1519	the development	1505:1519	the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp	1505:1631	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	6	42	theme	osteogenic	1253:1262	arg1	differentiation					1264:1278	osteogenic differentiation	1253:1278	osteogenic differentiation	1253:1278	Human MSCs were seeded on bare and coated scaffolds and cultured for up to 28 days to determine the adhesion, proliferation, migration and osteogenic differentiation.
36557882	3	43	theme	PEEK	544:547	arg1	matrices					549:556	Interconnected porous PEEK matrices	522:556	Interconnected porous PEEK matrices	522:556	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	7	44	theme	MSCs	1340:1343	arg1	adhesion					1345:1352	MSCs adhesion	1340:1352	MSCs adhesion	1340:1352	In vitro results showed that the MeHA-HAp coating promotes MSCs adhesion and proliferation and contributes to osteogenic differentiation and extracellular matrix mineralization.
36557882	5	45	theme	mechanical	788:797	arg1	properties					799:808	The mechanical properties	784:808	The mechanical properties of the coated and pure PEEK scaffold	784:845	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	8	46	theme	great	1549:1553	arg1	performances					1566:1577	the great mechanical performances	1545:1577	the great mechanical performances of PEEK	1545:1585	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	3	47	theme	reticular	626:634	arg1	pattern					636:642	a reticular pattern	624:642	a reticular pattern with 0°/90° raster orientation and square pores	624:690	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	3	48	theme	fused	575:579	arg1	FDM					602:604	FDM	602:604	FDM	602:604	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	3	48	theme	fused	575:579	arg1	modeling					592:599	fused deposition modeling	575:599	fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores	575:690	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	8	49	theme	HAp	1629:1631	arg1	properties					1606:1615	the bioactive properties	1592:1615	the bioactive properties of MeHA and HAp	1592:1631	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	1	50	theme	methacrylated	197:209	arg1	MeHA					228:231	MeHA	228:231	MeHA	228:231	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	1	50	theme	methacrylated	197:209	arg1	acid					222:225	methacrylated hyaluronic acid	197:225	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	0	51	theme	Printed	29:35	arg1	Structures					37:46	PEEK and Hyaluronan-Based 3D Printed Structures	0:46	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.	0:99	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.
36557882	5	52	theme	PEEK-MeHA-HAp	1075:1087	arg1	scaffolds					1089:1097	PEEK-MeHA-HAp scaffolds	1075:1097	PEEK-MeHA-HAp scaffolds	1075:1097	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	3	53	theme	deposition	581:590	arg1	FDM					602:604	FDM	602:604	FDM	602:604	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	3	53	theme	deposition	581:590	arg1	modeling					592:599	fused deposition modeling	575:599	fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores	575:690	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	1	54	theme	hyaluronic	211:220	arg1	MeHA					228:231	MeHA	228:231	MeHA	228:231	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	1	54	theme	hyaluronic	211:220	arg1	acid					222:225	methacrylated hyaluronic acid	197:225	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	0	55	theme	Promising	49:57	arg1	Combination					59:69	Promising Combination	49:69	Promising Combination	49:69	PEEK and Hyaluronan-Based 3D Printed Structures: Promising Combination to Improve Bone Regeneration.
36557882	5	56	theme	MPa	1036:1038	arg1	modulus					1010:1016	Young's modulus	1002:1016	Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively	1002:1111	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	5	57	theme	structure	987:995	arg1	strength					966:973	the strength	962:973	the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively	962:1111	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	2	58	theme	scaffold	342:349	arg1	characterization					318:333	characterization	318:333	characterization	318:333	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	2	58	theme	scaffold	342:349	arg1	Development					302:312	Development	302:312	Development	302:312	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	1	59	theme	present	288:294	arg1	work					296:299	the present work	284:299	the present work	284:299	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	5	60	theme	MPa	1058:1060	arg1	modulus					1010:1016	Young's modulus	1002:1016	Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively	1002:1111	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	3	61	theme	0°/90°	649:654	arg1	orientation					663:673	0°/90° raster orientation	649:673	0°/90° raster orientation	649:673	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	6	62	theme	coated	1149:1154	arg1	scaffolds					1156:1164	bare and coated scaffolds	1140:1164	bare and coated scaffolds	1140:1164	Human MSCs were seeded on bare and coated scaffolds and cultured for up to 28 days to determine the adhesion, proliferation, migration and osteogenic differentiation.
36557882	1	63	theme	work	296:299	arg1	objective					271:279	the objective	267:279	the objective of the present work	267:299	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	1	63	theme	work	296:299	arg1	bone					108:111	Hybrid bone	101:111	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	101:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	8	64	theme	MeHA	1620:1623	arg1	properties					1606:1615	the bioactive properties	1592:1615	the bioactive properties of MeHA and HAp	1592:1631	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	3	65	theme	raster	656:661	arg1	orientation					663:673	0°/90° raster orientation	649:673	0°/90° raster orientation	649:673	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	5	66	theme	PEEK	982:985	arg1	structure					987:995	the PEEK structure	978:995	the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively	978:1111	The mechanical properties of the coated and pure PEEK scaffold have been evaluated, showing that the inclusion of MeHA-HAp into the lattice geometry did not significantly change the strength of the PEEK structure with Young's modulus of 1034.9 ± 126.1 MPa and 1020.0 ± 63.7 MPa for PEEK and PEEK-MeHA-HAp scaffolds, respectively.
36557882	3	67	theme	porous	537:542	arg1	matrices					549:556	Interconnected porous PEEK matrices	522:556	Interconnected porous PEEK matrices	522:556	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	1	68	theme	acid	222:225	arg1	hydrogel					255:262	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	197:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	6	69	theme	bare	1140:1143	arg1	scaffolds					1156:1164	bare and coated scaffolds	1140:1164	bare and coated scaffolds	1140:1164	Human MSCs were seeded on bare and coated scaffolds and cultured for up to 28 days to determine the adhesion, proliferation, migration and osteogenic differentiation.
36557882	6	70	theme	Human	1114:1118	arg1	MSCs					1120:1123	Human MSCs	1114:1123	Human MSCs	1114:1123	Human MSCs were seeded on bare and coated scaffolds and cultured for up to 28 days to determine the adhesion, proliferation, migration and osteogenic differentiation.
36557882	1	71	theme	3D	137:138	arg1	scaffold					176:183	a 3D printed polyetheretherketone (PEEK) scaffold	135:183	a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	135:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	2	72	theme	human	485:489	arg1	MSCs					515:518	MSCs	515:518	MSCs	515:518	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	2	72	theme	human	485:489	arg1	cells					508:512	human mesenchymal stem cells	485:512	human mesenchymal stem cells (MSCs)	485:519	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	2	73	theme	MeHA-HAp	362:369	arg1	characterization					318:333	characterization	318:333	characterization	318:333	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	2	73	theme	MeHA-HAp	362:369	arg1	Development					302:312	Development	302:312	Development	302:312	Development and characterization of the scaffold and of the MeHA-HAp after its infiltration and UV photocrosslinking have been followed by analyses of its biological properties using human mesenchymal stem cells (MSCs).
36557882	3	74	theme	square	679:684	arg1	pores					686:690	square pores	679:690	square pores	679:690	Interconnected porous PEEK matrices were produced by fused deposition modeling (FDM) characterized by a reticular pattern with 0°/90° raster orientation and square pores.
36557882	4	75	theme	PEEK	768:771	arg1	scaffolds					773:781	the PEEK scaffolds	764:781	the PEEK scaffolds	764:781	In parallel, a MeHA-HAp slurry has been synthesized and infiltrated in the PEEK scaffolds.
36557882	1	76	dep	bone	108:111	arg1	substitute					113:122	substitute	113:122	substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel	113:262	Hybrid bone substitute made up of a 3D printed polyetheretherketone (PEEK) scaffold coated with methacrylated hyaluronic acid (MeHA)-hydroxyapatite (HAp) hydrogel is the objective of the present work.
36557882	8	77	theme	high	1641:1644	arg1	potential					1646:1654	high potential	1641:1654	high potential for translational clinical applications	1641:1694	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
36557882	8	78	theme	PEEK	1582:1585	arg1	performances					1566:1577	the great mechanical performances	1545:1577	the great mechanical performances of PEEK	1545:1585	This study provides an efficient solution for the development of a scaffold combining the great mechanical performances of PEEK with the bioactive properties of MeHA and HAp, having high potential for translational clinical applications.
35575226	3	0	theme	AOM	666:668	arg1	day					649:651	the day	645:651	the day of receiving AOM	645:668	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	8	1	from	content	1526:1532	arg1	samples					1570:1576	fecal samples	1564:1576	fecal samples	1564:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	1	2	theme	cerevisiae	302:311	arg1	effects					230:236	the alleviative effects	214:236	the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis	214:409	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	2	3	used	used	469:472	arg2	DSS					459:461	DSS	459:461	DSS	459:461	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	2	3	used	used	469:472	arg2	AOM					426:428	AOM	426:428	AOM	426:428	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	2	3	used	used	469:472	arg2	sodium					451:456	dextran sulfate sodium	435:456	dextran sulfate sodium (DSS)	435:462	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	2	3	used	used	469:472	arg2	Azoxymethane					412:423	Azoxymethane	412:423	Azoxymethane (AOM)	412:429	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	1	4	attach	isolated	325:332	arg2	SC					321:322	SC	321:322	SC	321:322	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	1	4	attach	isolated	325:332	arg2	kefiranofaciens					255:269	Lactobacillus kefiranofaciens	241:269	Lactobacillus kefiranofaciens JKSP109 (LK)	241:282	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	1	4	attach	isolated	325:332	arg1	grain					353:357	Tibetan kefir grain	339:357	Tibetan kefir grain	339:357	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	1	4	attach	isolated	325:332	arg2	LK					280:281	LK	280:281	LK	280:281	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	5	5	theme	gut	848:850	arg1	integrity					860:868	gut barrier integrity	848:868	gut barrier integrity	848:868	Furthermore, the biomarkers associated with gut barrier integrity, inflammation, regulators of cell proliferation, and apoptosis were evaluated.
35575226	9	6	from	inflammation	1641:1652	arg1	models					1713:1718	AOM/DSS-induced CRC mouse models	1687:1718	AOM/DSS-induced CRC mouse models	1687:1718	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	0	7	attach	isolated	74:81	arg1	AOM/DSS					122:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	0	7	attach	isolated	74:81	arg2	JKSP109					30:36	Lactobacillus kefiranofaciens JKSP109	0:36	Lactobacillus kefiranofaciens JKSP109	0:36	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	8	8	from	samples	1570:1576	arg1	expression					1407:1416	the expression	1403:1416	the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1403:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	8	from	samples	1570:1576	arg1	content					1526:1532	content	1526:1532	content of short chain fatty acids in fecal samples	1526:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	9	9	theme	AOM/DSS-induced	1687:1701	arg1	models					1713:1718	AOM/DSS-induced CRC mouse models	1687:1718	AOM/DSS-induced CRC mouse models	1687:1718	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	9	10	theme	mouse	1707:1711	arg1	models					1713:1718	AOM/DSS-induced CRC mouse models	1687:1718	AOM/DSS-induced CRC mouse models	1687:1718	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	8	11	theme	terminal	1421:1428	arg1	TUNEL					1483:1487	TUNEL	1483:1487	TUNEL	1483:1487	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	11	theme	terminal	1421:1428	arg1	labeling					1473:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling	1421:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	5	12	theme	cell	899:902	arg1	proliferation					904:916	cell proliferation	899:916	cell proliferation	899:916	Furthermore, the biomarkers associated with gut barrier integrity, inflammation, regulators of cell proliferation, and apoptosis were evaluated.
35575226	6	13	theme	LK	995:996	arg1	administration					977:990	the administration	973:990	the administration of LK, SC, and their combination	973:1023	The results showed that the administration of LK, SC, and their combination increased the body weights and decreased the disease activity index (DAI) score and tumor multiplicity.
35575226	8	14	theme	transferase	1447:1457	arg1	TUNEL					1483:1487	TUNEL	1483:1487	TUNEL	1483:1487	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	14	theme	transferase	1447:1457	arg1	labeling					1473:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling	1421:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	15	from	expression	1407:1416	arg1	samples					1570:1576	fecal samples	1564:1576	fecal samples	1564:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	1	16	theme	colon	362:366	arg1	inflammation					368:379	colon inflammation	362:379	colon inflammation	362:379	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	2	17	theme	colorectal	504:513	arg1	cancer					515:520	colorectal cancer	504:520	colorectal cancer (CRC)	504:526	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	2	17	theme	colorectal	504:513	arg1	CRC					523:525	CRC	523:525	CRC	523:525	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	8	18	theme	nick-end	1464:1471	arg1	TUNEL					1483:1487	TUNEL	1483:1487	TUNEL	1483:1487	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	18	theme	nick-end	1464:1471	arg1	labeling					1473:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling	1421:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	0	19	theme	kefir	96:100	arg1	AOM/DSS					122:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	8	20	theme	gut	1283:1285	arg1	barrier					1287:1293	the gut barrier	1279:1293	the gut barrier	1279:1293	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	0	21	theme	co-alleviated	108:120	arg1	AOM/DSS					122:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	9	22	theme	indexes	1748:1754	arg1	majority					1729:1736	the majority	1725:1736	the majority of tested indexes in the combination group	1725:1779	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	9	22	theme	indexes	1748:1754	arg1	superior					1786:1793	superior	1786:1793	superior	1786:1793	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	4	23	theme	transcribed	737:747	arg1	spacer					749:754	internal transcribed spacer	728:754	internal transcribed spacer 2	728:756	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	2	24	theme	dextran	435:441	arg1	sodium					451:456	dextran sulfate sodium	435:456	dextran sulfate sodium (DSS)	435:462	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	2	24	theme	dextran	435:441	arg1	DSS					459:461	DSS	459:461	DSS	459:461	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	8	25	theme	tumor	1499:1503	arg1	cells					1516:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	4	26	theme	gut	694:696	arg1	microbiota					698:707	the gut microbiota	690:707	the gut microbiota	690:707	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	0	27	theme	Lactobacillus	0:12	arg1	JKSP109					30:36	Lactobacillus kefiranofaciens JKSP109	0:36	Lactobacillus kefiranofaciens JKSP109	0:36	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	6	28	theme	combination	1013:1023	arg1	administration					977:990	the administration	973:990	the administration of LK, SC, and their combination	973:1023	The results showed that the administration of LK, SC, and their combination increased the body weights and decreased the disease activity index (DAI) score and tumor multiplicity.
35575226	8	29	theme	cells	1516:1520	arg1	expression					1407:1416	the expression	1403:1416	the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1403:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	29	theme	cells	1516:1520	arg1	content					1526:1532	content	1526:1532	content of short chain fatty acids in fecal samples	1526:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	4	30	theme	gene	771:774	arg1	sequencing					792:801	16S rRNA gene high-throughput sequencing	762:801	16S rRNA gene high-throughput sequencing	762:801	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	9	31	from	indexes	1748:1754	arg1	group					1775:1779	the combination group	1759:1779	the combination group	1759:1779	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	8	32	theme	cytokines	1340:1348	arg1	expression					1310:1319	the expression	1306:1319	the expression of proinflammatory cytokines and oncocyte proliferation indicators	1306:1386	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	0	33	theme	Saccharomyces	42:54	arg1	JKSP39					67:72	Saccharomyces cerevisiae JKSP39	42:72	Saccharomyces cerevisiae JKSP39	42:72	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	1	34	theme	Tibetan	339:345	arg1	grain					353:357	Tibetan kefir grain	339:357	Tibetan kefir grain	339:357	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	9	35	dep	inflammation	1641:1652	arg1	the					1637:1639	the	1637:1639	the	1637:1639	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	6	36	theme	SC	999:1000	arg1	administration					977:990	the administration	973:990	the administration of LK, SC, and their combination	973:1023	The results showed that the administration of LK, SC, and their combination increased the body weights and decreased the disease activity index (DAI) score and tumor multiplicity.
35575226	9	37	theme	single	1798:1803	arg1	groups					1812:1817	single strain groups	1798:1817	single strain groups	1798:1817	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	8	38	theme	short	1537:1541	arg1	acids					1555:1559	short chain fatty acids	1537:1559	short chain fatty acids in fecal samples	1537:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	39	theme	oncocyte	1354:1361	arg1	indicators					1377:1386	oncocyte proliferation indicators	1354:1386	oncocyte proliferation indicators	1354:1386	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	0	40	dep	Saccharomyces	42:54	arg1	cerevisiae					56:65	cerevisiae	56:65	cerevisiae	56:65	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	8	41	theme	fatty	1549:1553	arg1	acids					1555:1559	short chain fatty acids	1537:1559	short chain fatty acids in fecal samples	1537:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	42	theme	indicators	1377:1386	arg1	expression					1310:1319	the expression	1306:1319	the expression of proinflammatory cytokines and oncocyte proliferation indicators	1306:1386	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	43	theme	proinflammatory	1324:1338	arg1	cytokines					1340:1348	proinflammatory cytokines	1324:1348	proinflammatory cytokines	1324:1348	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	3	44	theme	group	543:547	arg1	mice					549:552	The treatment group mice	529:552	The treatment group mice	529:552	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	6	45	theme	disease	1070:1076	arg1	index					1087:1091	the disease activity index	1066:1091	the disease activity index	1066:1091	The results showed that the administration of LK, SC, and their combination increased the body weights and decreased the disease activity index (DAI) score and tumor multiplicity.
35575226	4	46	theme	16S	762:764	arg1	sequencing					792:801	16S rRNA gene high-throughput sequencing	762:801	16S rRNA gene high-throughput sequencing	762:801	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	0	47	dep	Lactobacillus	0:12	arg1	kefiranofaciens					14:28	kefiranofaciens	14:28	kefiranofaciens	14:28	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	7	48	theme	model	1152:1156	arg1	group					1158:1162	the CRC model group	1144:1162	the CRC model group	1144:1162	As compared to the CRC model group, the three treatment groups positively regulated the gut microbiota.
35575226	7	49	theme	treatment	1175:1183	arg1	groups					1185:1190	the three treatment groups	1165:1190	the three treatment groups	1165:1190	As compared to the CRC model group, the three treatment groups positively regulated the gut microbiota.
35575226	9	50	from	group	1775:1779	arg1	majority					1729:1736	the majority	1725:1736	the majority of tested indexes in the combination group	1725:1779	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	9	50	from	group	1775:1779	arg1	superior					1786:1793	superior	1786:1793	superior	1786:1793	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	8	51	from	acids	1555:1559	arg1	samples					1570:1576	fecal samples	1564:1576	fecal samples	1564:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	3	52	theme	treatment	533:541	arg1	group					543:547	The treatment group	529:547	The treatment group mice	529:552	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	6	53	theme	tumor	1109:1113	arg1	multiplicity					1115:1126	tumor multiplicity	1109:1126	tumor multiplicity	1109:1126	The results showed that the administration of LK, SC, and their combination increased the body weights and decreased the disease activity index (DAI) score and tumor multiplicity.
35575226	1	54	theme	alleviative	218:228	arg1	effects					230:236	the alleviative effects	214:236	the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis	214:409	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	0	55	theme	colorectal	155:164	arg1	carcinogenesis					166:179	colorectal carcinogenesis	155:179	colorectal carcinogenesis	155:179	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	9	56	theme	colorectal	1658:1667	arg1	carcinogenesis					1669:1682	colorectal carcinogenesis	1658:1682	colorectal carcinogenesis	1658:1682	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	3	57	from	day	649:651	arg1	week					635:638	week	635:638	week from the day of receiving AOM	635:668	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	1	58	theme	kefiranofaciens	255:269	arg1	effects					230:236	the alleviative effects	214:236	the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis	214:409	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	9	59	theme	CRC	1703:1705	arg1	models					1713:1718	AOM/DSS-induced CRC mouse models	1687:1718	AOM/DSS-induced CRC mouse models	1687:1718	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	8	60	theme	deoxynucleotidyl	1430:1445	arg1	TUNEL					1483:1487	TUNEL	1483:1487	TUNEL	1483:1487	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	60	theme	deoxynucleotidyl	1430:1445	arg1	labeling					1473:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling	1421:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	1	61	dep	kefiranofaciens	255:269	arg1	JKSP39					313:318	JKSP39	313:318	JKSP39	313:318	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	1	61	dep	kefiranofaciens	255:269	arg1	JKSP109					271:277	JKSP109	271:277	JKSP109	271:277	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	3	62	theme	LK	607:608	arg1	SC					581:582	SC	581:582	SC	581:582	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	3	62	theme	LK	607:608	arg1	LK					577:578	LK	577:578	LK	577:578	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	3	62	theme	LK	607:608	arg1	combination					592:602	the combination	588:602	the combination of LK and SC for five days per week from the day of receiving AOM	588:668	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	8	63	theme	dUTP	1459:1462	arg1	TUNEL					1483:1487	TUNEL	1483:1487	TUNEL	1483:1487	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	63	theme	dUTP	1459:1462	arg1	labeling					1473:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling	1421:1480	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	0	64	theme	Tibetan	88:94	arg1	AOM/DSS					122:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	1	65	from	effects	230:236	arg1	inflammation					368:379	colon inflammation	362:379	colon inflammation	362:379	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	1	65	from	effects	230:236	arg1	carcinogenesis					396:409	colorectal carcinogenesis	385:409	colorectal carcinogenesis	385:409	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	5	66	theme	barrier	852:858	arg1	integrity					860:868	gut barrier integrity	848:868	gut barrier integrity	848:868	Furthermore, the biomarkers associated with gut barrier integrity, inflammation, regulators of cell proliferation, and apoptosis were evaluated.
35575226	2	67	theme	cancer	515:520	arg1	model					495:499	a mouse model	487:499	a mouse model of colorectal cancer (CRC)	487:526	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	8	68	theme	labeling	1473:1480	arg1	cells					1516:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	7	69	theme	CRC	1148:1150	arg1	group					1158:1162	the CRC model group	1144:1162	the CRC model group	1144:1162	As compared to the CRC model group, the three treatment groups positively regulated the gut microbiota.
35575226	0	70	theme	grain	102:106	arg1	AOM/DSS					122:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Tibetan kefir grain co-alleviated AOM/DSS	88:128	Lactobacillus kefiranofaciens JKSP109 and Saccharomyces cerevisiae JKSP39 isolated from Tibetan kefir grain co-alleviated AOM/DSS induced inflammation and colorectal carcinogenesis.
35575226	9	71	from	carcinogenesis	1669:1682	arg1	models					1713:1718	AOM/DSS-induced CRC mouse models	1687:1718	AOM/DSS-induced CRC mouse models	1687:1718	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	1	72	theme	colorectal	385:394	arg1	carcinogenesis					396:409	colorectal carcinogenesis	385:409	colorectal carcinogenesis	385:409	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	9	73	theme	tested	1741:1746	arg1	indexes					1748:1754	tested indexes	1741:1754	tested indexes in the combination group	1741:1779	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	8	74	theme	-positive	1489:1497	arg1	cells					1516:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	6	75	theme	body	1039:1042	arg1	weights					1044:1050	the body weights	1035:1050	the body weights	1035:1050	The results showed that the administration of LK, SC, and their combination increased the body weights and decreased the disease activity index (DAI) score and tumor multiplicity.
35575226	8	76	theme	epithelial	1505:1514	arg1	cells					1516:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1421:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	4	77	theme	internal	728:735	arg1	spacer					749:754	internal transcribed spacer	728:754	internal transcribed spacer 2	728:756	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	9	78	dep	LK	1616:1617	arg1	the					1612:1614	the	1612:1614	the	1612:1614	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	5	79	theme	proliferation	904:916	arg1	apoptosis					923:931	apoptosis	923:931	apoptosis	923:931	Furthermore, the biomarkers associated with gut barrier integrity, inflammation, regulators of cell proliferation, and apoptosis were evaluated.
35575226	5	79	theme	proliferation	904:916	arg1	regulators					885:894	regulators	885:894	regulators of cell proliferation	885:916	Furthermore, the biomarkers associated with gut barrier integrity, inflammation, regulators of cell proliferation, and apoptosis were evaluated.
35575226	5	79	theme	proliferation	904:916	arg1	inflammation					871:882	inflammation	871:882	inflammation	871:882	Furthermore, the biomarkers associated with gut barrier integrity, inflammation, regulators of cell proliferation, and apoptosis were evaluated.
35575226	5	79	theme	proliferation	904:916	arg1	integrity					860:868	gut barrier integrity	848:868	gut barrier integrity	848:868	Furthermore, the biomarkers associated with gut barrier integrity, inflammation, regulators of cell proliferation, and apoptosis were evaluated.
35575226	9	80	theme	combination	1763:1773	arg1	group					1775:1779	the combination group	1759:1779	the combination group	1759:1779	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	4	81	theme	microbiota	698:707	arg1	composition					675:685	The composition	671:685	The composition of the gut microbiota	671:707	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	2	82	theme	mouse	489:493	arg1	model					495:499	a mouse model	487:499	a mouse model of colorectal cancer (CRC)	487:526	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	8	83	theme	chain	1543:1547	arg1	acids					1555:1559	short chain fatty acids	1537:1559	short chain fatty acids in fecal samples	1537:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	3	84	theme	SC	614:615	arg1	SC					581:582	SC	581:582	SC	581:582	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	3	84	theme	SC	614:615	arg1	LK					577:578	LK	577:578	LK	577:578	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	3	84	theme	SC	614:615	arg1	combination					592:602	the combination	588:602	the combination of LK and SC for five days per week from the day of receiving AOM	588:668	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
35575226	4	85	theme	rRNA	766:769	arg1	sequencing					792:801	16S rRNA gene high-throughput sequencing	762:801	16S rRNA gene high-throughput sequencing	762:801	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	2	86	theme	sulfate	443:449	arg1	sodium					451:456	dextran sulfate sodium	435:456	dextran sulfate sodium (DSS)	435:462	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	2	86	theme	sulfate	443:449	arg1	DSS					459:461	DSS	459:461	DSS	459:461	Azoxymethane (AOM) and dextran sulfate sodium (DSS) were used to establish a mouse model of colorectal cancer (CRC).
35575226	1	87	theme	kefir	347:351	arg1	grain					353:357	Tibetan kefir grain	339:357	Tibetan kefir grain	339:357	This study aimed to investigate the alleviative effects of Lactobacillus kefiranofaciens JKSP109 (LK) and Saccharomyces cerevisiae JKSP39 (SC) isolated from Tibetan kefir grain on colon inflammation and colorectal carcinogenesis.
35575226	7	88	theme	gut	1217:1219	arg1	microbiota					1221:1230	the gut microbiota	1213:1230	the gut microbiota	1213:1230	As compared to the CRC model group, the three treatment groups positively regulated the gut microbiota.
35575226	9	89	theme	strain	1805:1810	arg1	groups					1812:1817	single strain groups	1798:1817	single strain groups	1798:1817	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	8	90	theme	acids	1555:1559	arg1	expression					1407:1416	the expression	1403:1416	the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells	1403:1520	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	90	theme	acids	1555:1559	arg1	content					1526:1532	content	1526:1532	content of short chain fatty acids in fecal samples	1526:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	4	91	theme	high-throughput	776:790	arg1	sequencing					792:801	16S rRNA gene high-throughput sequencing	762:801	16S rRNA gene high-throughput sequencing	762:801	The composition of the gut microbiota was assessed using internal transcribed spacer 2 and 16S rRNA gene high-throughput sequencing.
35575226	9	92	from	majority	1729:1736	arg1	group					1775:1779	the combination group	1759:1779	the combination group	1759:1779	All these results indicated that the LK and SC alleviated the inflammation and colorectal carcinogenesis in AOM/DSS-induced CRC mouse models, and the majority of tested indexes in the combination group were superior to single strain groups.
35575226	6	93	theme	activity	1078:1085	arg1	index					1087:1091	the disease activity index	1066:1091	the disease activity index	1066:1091	The results showed that the administration of LK, SC, and their combination increased the body weights and decreased the disease activity index (DAI) score and tumor multiplicity.
35575226	8	94	theme	fecal	1564:1568	arg1	samples					1570:1576	fecal samples	1564:1576	fecal samples	1564:1576	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	8	95	theme	proliferation	1363:1375	arg1	indicators					1377:1386	oncocyte proliferation indicators	1354:1386	oncocyte proliferation indicators	1354:1386	Meanwhile, the three treatments also enhanced the gut barrier, decreased the expression of proinflammatory cytokines and oncocyte proliferation indicators, and increased the expression of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL)-positive tumor epithelial cells and content of short chain fatty acids in fecal samples.
35575226	3	96	theme	receiving	656:664	arg1	AOM					666:668	receiving AOM	656:668	receiving AOM	656:668	The treatment group mice were administered with LK, SC, or the combination of LK and SC for five days per week from the day of receiving AOM.
36251859	7	0	theme	bone	1377:1380	arg1	density					1390:1396	bone mineral density	1377:1396	bone mineral density	1377:1396	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	10	1	theme	current	1853:1859	arg1	study					1861:1865	The current study	1849:1865	The current study	1849:1865	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	3	2	theme	core	630:633	arg1	shell					635:639	core@shell	630:639	core@shell	630:639	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	3	2	theme	core	630:633	arg1	HA					615:616	BCP@HA	611:616	BCP@HA	611:616	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	7	3	theme	animal	1323:1328	arg1	model					1330:1334	a critical-sized femoral bone defect animal model	1286:1334	a critical-sized femoral bone defect animal model	1286:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	6	4	theme	stem	1164:1167	arg1	cells					1169:1173	bone marrow mesenchymal stem cells	1140:1173	bone marrow mesenchymal stem cells	1140:1173	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	3	5	theme	bioceramic	517:526	arg1	granules					528:535	two porous CaP bioceramic granules	502:535	two porous CaP bioceramic granules	502:535	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	7	6	theme	bone	1352:1355	arg1	fraction					1364:1371	a higher bone volume fraction	1343:1371	a higher bone volume fraction	1343:1371	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	7	7	theme	bone	1311:1314	arg1	model					1330:1334	a critical-sized femoral bone defect animal model	1286:1334	a critical-sized femoral bone defect animal model	1286:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	6	8	theme	marrow	1145:1150	arg1	cells					1169:1173	bone marrow mesenchymal stem cells	1140:1173	bone marrow mesenchymal stem cells	1140:1173	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	3	9	theme	porous	506:511	arg1	granules					528:535	two porous CaP bioceramic granules	502:535	two porous CaP bioceramic granules	502:535	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	8	10	theme	@	1539:1539	arg1	group					1544:1548	the HA@BCP group	1533:1548	the HA@BCP group	1533:1548	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	4	11	theme	H2O2	783:786	arg1	process					796:802	a H2O2 foaming process	781:802	a H2O2 foaming process	781:802	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	6	12	theme	@	1039:1039	arg1	granules					1055:1062	HA@BCP bioceramic granules	1037:1062	HA@BCP bioceramic granules	1037:1062	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	9	13	theme	vascularization	1693:1707	arg1	degree					1683:1688	A higher degree	1674:1688	A higher degree of vascularization at the defect region repaired by HA@BCP	1674:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	2	14	theme	calcium	306:312	arg1	BCPs					326:329	BCPs	326:329	BCPs	326:329	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	14	theme	calcium	306:312	arg1	phosphates					314:323	biphasic calcium phosphates	297:323	biphasic calcium phosphates (BCPs)	297:330	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	1	15	theme	excellent	188:196	arg1	osteogenesis					212:223	excellent and efficient osteogenesis	188:223	excellent and efficient osteogenesis	188:223	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	10	16	theme	@	1913:1913	arg1	candidate					1959:1967	a promising candidate	1947:1967	a promising candidate for bone defect repair	1947:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	10	16	theme	@	1913:1913	arg1	granules					1929:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	1	17	theme	efficient	202:210	arg1	osteogenesis					212:223	excellent and efficient osteogenesis	188:223	excellent and efficient osteogenesis	188:223	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	10	18	theme	structured	1900:1909	arg1	candidate					1959:1967	a promising candidate	1947:1967	a promising candidate for bone defect repair	1947:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	10	18	theme	structured	1900:1909	arg1	granules					1929:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	6	19	theme	RAW264.7	1216:1223	arg1	cells					1225:1229	RAW264.7 cells	1216:1229	RAW264.7 cells	1216:1229	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	2	20	theme	Calcium	226:232	arg1	CaP					245:247	CaP	245:247	CaP	245:247	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	20	theme	Calcium	226:232	arg1	phosphate					234:242	Calcium phosphate	226:242	Calcium phosphate (CaP) bioceramics	226:260	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	1	21	theme	bone	132:135	arg1	defects					137:143	bone defects	132:143	bone defects	132:143	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	9	22	theme	@	1744:1744	arg1	BCP					1745:1747	HA@BCP	1742:1747	HA@BCP	1742:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	10	23	theme	bioceramic	1918:1927	arg1	candidate					1959:1967	a promising candidate	1947:1967	a promising candidate for bone defect repair	1947:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	10	23	theme	bioceramic	1918:1927	arg1	granules					1929:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	5	24	theme	porous	848:853	arg1	structure					855:863	a porous structure	846:863	a porous structure with a porosity of 65-70%	846:889	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	7	25	theme	pure	1440:1443	arg1	HA					1445:1446	pure HA or BCP groups	1440:1460	HA	1445:1446	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	0	26	theme	Enhanced	70:77	arg1	Regeneration					84:95	Enhanced Bone Regeneration	70:95	Enhanced Bone Regeneration	70:95	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	7	27	theme	BCP	1430:1432	arg1	HA					1434:1435	the BCP@HA	1426:1435	the BCP@HA	1426:1435	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	9	28	theme	microvascular	1768:1780	arg1	angiography					1792:1802	3D microvascular perfusion angiography	1765:1802	3D microvascular perfusion angiography in terms of a higher vessel volume fraction	1765:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	10	29	theme	core-shell	1889:1898	arg1	candidate					1959:1967	a promising candidate	1947:1967	a promising candidate for bone defect repair	1947:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	10	29	theme	core-shell	1889:1898	arg1	granules					1929:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	2	30	used	used	355:358	arg2	phosphates					314:323	biphasic calcium phosphates	297:323	biphasic calcium phosphates (BCPs)	297:330	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	30	used	used	355:358	arg2	hydroxyapatite					273:286	hydroxyapatite	273:286	hydroxyapatite (HA)	273:291	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	30	used	used	355:358	arg2	bioceramics					250:260	Calcium phosphate (CaP) bioceramics	226:260	Calcium phosphate (CaP) bioceramics	226:260	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	31	theme	defect	377:382	arg1	filling					384:390	clinical bone defect filling	363:390	clinical bone defect filling due to their good osteoinductivity and biodegradability	363:446	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	5	32	theme	bone	986:989	arg1	ingrowth					991:998	new bone ingrowth	982:998	new bone ingrowth	982:998	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	8	33	from	tissue	1523:1528	arg1	group					1544:1548	the HA@BCP group	1533:1548	the HA@BCP group	1533:1548	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	2	34	theme	clinical	363:370	arg1	filling					384:390	clinical bone defect filling	363:390	clinical bone defect filling due to their good osteoinductivity and biodegradability	363:446	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	0	35	theme	Core-Shell	0:9	arg1	Spheres					58:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres	0:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.	0:96	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	1	36	theme	new	107:109	arg1	challenge					163:171	a challenge	161:171	a challenge	161:171	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	1	36	theme	new	107:109	arg1	regeneration					116:127	Adequate new bone regeneration	98:127	Adequate new bone regeneration in bone defects	98:143	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	0	37	theme	Porous	22:27	arg1	Spheres					58:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres	0:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.	0:96	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	6	38	theme	bioceramic	1044:1053	arg1	granules					1055:1062	HA@BCP bioceramic granules	1037:1062	HA@BCP bioceramic granules	1037:1062	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	7	39	theme	BCP	1408:1410	arg1	group					1412:1416	the HA@BCP group	1401:1416	the HA@BCP group	1401:1416	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	9	40	theme	vessel	1825:1830	arg1	fraction					1839:1846	a higher vessel volume fraction	1816:1846	a higher vessel volume fraction	1816:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	4	41	theme	gel	744:746	arg1	technology					756:765	the calcium alginate gel molding technology	723:765	the calcium alginate gel molding technology combined with a H2O2 foaming process	723:802	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	0	42	theme	Phosphate	37:45	arg1	Spheres					58:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres	0:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.	0:96	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	7	43	theme	HA	1405:1406	arg1	group					1412:1416	the HA@BCP group	1401:1416	the HA@BCP group	1401:1416	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	3	44	theme	@	614:614	arg1	HA					615:616	BCP@HA	611:616	BCP@HA	611:616	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	3	44	theme	@	614:614	arg1	shell					635:639	core@shell	630:639	core@shell	630:639	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	8	45	theme	new	1664:1666	arg1	bone					1668:1671	the new bone	1660:1671	the new bone	1660:1671	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	9	46	theme	volume	1832:1837	arg1	fraction					1839:1846	a higher vessel volume fraction	1816:1846	a higher vessel volume fraction	1816:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	6	47	dep	In	1001:1002	arg1	vitro					1004:1008	vitro	1004:1008	vitro	1004:1008	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	1	48	from	regeneration	116:127	arg1	defects					137:143	bone defects	132:143	bone defects	132:143	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	7	49	theme	defect	1316:1321	arg1	model					1330:1334	a critical-sized femoral bone defect animal model	1286:1334	a critical-sized femoral bone defect animal model	1286:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	7	50	theme	mineral	1382:1388	arg1	density					1390:1396	bone mineral density	1377:1396	bone mineral density	1377:1396	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	5	51	theme	micropore	897:905	arg1	distribution					912:923	a micropore size distribution	895:923	a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth	895:998	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	3	52	theme	@	624:624	arg1	BCP					625:627	HA@BCP	622:627	HA@BCP	622:627	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	4	53	theme	calcium	727:733	arg1	technology					756:765	the calcium alginate gel molding technology	723:765	the calcium alginate gel molding technology combined with a H2O2 foaming process	723:802	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	9	54	from	angiography	1792:1802	arg1	terms					1807:1811	terms	1807:1811	terms of a higher vessel volume fraction	1807:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	7	55	theme	volume	1357:1362	arg1	fraction					1364:1371	a higher bone volume fraction	1343:1371	a higher bone volume fraction	1343:1371	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	4	56	theme	foaming	788:794	arg1	process					796:802	a H2O2 foaming process	781:802	a H2O2 foaming process	781:802	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	9	57	theme	fraction	1839:1846	arg1	terms					1807:1811	terms	1807:1811	terms of a higher vessel volume fraction	1807:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	3	58	theme	@	634:634	arg1	shell					635:639	core@shell	630:639	core@shell	630:639	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	3	58	theme	@	634:634	arg1	HA					615:616	BCP@HA	611:616	BCP@HA	611:616	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	3	59	theme	CaP	513:515	arg1	granules					528:535	two porous CaP bioceramic granules	502:535	two porous CaP bioceramic granules	502:535	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	8	60	theme	bone	1518:1521	arg1	invading					1554:1561	invading	1554:1561	invading	1554:1561	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	8	60	theme	bone	1518:1521	arg1	tissue					1523:1528	the new bone tissue	1510:1528	the new bone tissue in the HA@BCP group	1510:1548	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	7	61	theme	critical-sized	1288:1301	arg1	model					1330:1334	a critical-sized femoral bone defect animal model	1286:1334	a critical-sized femoral bone defect animal model	1286:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	7	62	theme	higher	1345:1350	arg1	fraction					1364:1371	a higher bone volume fraction	1343:1371	a higher bone volume fraction	1343:1371	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	9	63	theme	higher	1676:1681	arg1	degree					1683:1688	A higher degree	1674:1688	A higher degree of vascularization at the defect region repaired by HA@BCP	1674:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	8	64	theme	histological	1468:1479	arg1	analysis					1481:1488	histological analysis	1468:1488	histological analysis	1468:1488	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	6	65	theme	mesenchymal	1152:1162	arg1	cells					1169:1173	bone marrow mesenchymal stem cells	1140:1173	bone marrow mesenchymal stem cells	1140:1173	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	6	66	theme	BCP	1040:1042	arg1	granules					1055:1062	HA@BCP bioceramic granules	1037:1062	HA@BCP bioceramic granules	1037:1062	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	8	67	from	surface	1572:1578	arg1	tissue					1523:1528	the new bone tissue	1510:1528	the new bone tissue in the HA@BCP group	1510:1548	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	8	67	from	surface	1572:1578	arg1	invading					1554:1561	invading	1554:1561	invading	1554:1561	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	6	68	theme	bone	1140:1143	arg1	marrow					1145:1150	bone marrow	1140:1150	bone marrow mesenchymal stem cells	1140:1173	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	3	69	theme	core-shell	542:551	arg1	structures					553:562	core-shell structures	542:562	core-shell structures	542:562	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	8	70	theme	HA	1537:1538	arg1	group					1544:1548	the HA@BCP group	1533:1548	the HA@BCP group	1533:1548	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	7	71	theme	femoral	1303:1309	arg1	model					1330:1334	a critical-sized femoral bone defect animal model	1286:1334	a critical-sized femoral bone defect animal model	1286:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	10	72	theme	BCP	1914:1916	arg1	candidate					1959:1967	a promising candidate	1947:1967	a promising candidate for bone defect repair	1947:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	10	72	theme	BCP	1914:1916	arg1	granules					1929:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	5	73	with	structure	855:863	arg1	porosity					872:879	a porosity	870:879	a porosity of 65-70%	870:889	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	2	74	theme	biphasic	297:304	arg1	BCPs					326:329	BCPs	326:329	BCPs	326:329	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	74	theme	biphasic	297:304	arg1	phosphates					314:323	biphasic calcium phosphates	297:323	biphasic calcium phosphates (BCPs)	297:330	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	8	75	theme	BCP	1540:1542	arg1	group					1544:1548	the HA@BCP group	1533:1548	the HA@BCP group	1533:1548	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	6	76	theme	HA	1037:1038	arg1	granules					1055:1062	HA@BCP bioceramic granules	1037:1062	HA@BCP bioceramic granules	1037:1062	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	9	77	theme	defect	1716:1721	arg1	region					1723:1728	the defect region	1712:1728	the defect region repaired by HA@BCP	1712:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	6	78	theme	cells	1225:1229	arg1	differentiation					1197:1211	the differentiation	1193:1211	the differentiation of RAW264.7 cells into osteoclasts	1193:1246	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	7	79	theme	BCP	1451:1453	arg1	groups					1455:1460	pure HA or BCP groups	1440:1460	groups	1455:1460	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	7	80	theme	implantation	1270:1281	arg1	weeks					1261:1265	12 weeks	1258:1265	12 weeks of implantation in a critical-sized femoral bone defect animal model	1258:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	2	81	theme	phosphate	234:242	arg1	bioceramics					250:260	Calcium phosphate (CaP) bioceramics	226:260	Calcium phosphate (CaP) bioceramics	226:260	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	81	theme	phosphate	234:242	arg1	hydroxyapatite					273:286	hydroxyapatite	273:286	hydroxyapatite (HA)	273:291	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	2	81	theme	phosphate	234:242	arg1	phosphates					314:323	biphasic calcium phosphates	297:323	biphasic calcium phosphates (BCPs)	297:330	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	6	82	theme	osteogenic	1100:1109	arg1	ability					1111:1117	osteogenic ability	1100:1117	osteogenic ability	1100:1117	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	9	83	theme	HA	1742:1743	arg1	BCP					1745:1747	HA@BCP	1742:1747	HA@BCP	1742:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	10	84	theme	defect	1978:1983	arg1	repair					1985:1990	bone defect repair	1973:1990	bone defect repair	1973:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	4	85	theme	spherical	647:655	arg1	shape					657:661	The spherical shape	643:661	The spherical shape	643:661	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	0	86	theme	Bone	79:82	arg1	Regeneration					84:95	Enhanced Bone Regeneration	70:95	Enhanced Bone Regeneration	70:95	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	7	87	theme	@	1433:1433	arg1	HA					1434:1435	the BCP@HA	1426:1435	the BCP@HA	1426:1435	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	9	88	from	degree	1683:1688	arg1	region					1723:1728	the defect region	1712:1728	the defect region repaired by HA@BCP	1712:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	10	89	theme	promising	1949:1957	arg1	candidate					1959:1967	a promising candidate	1947:1967	a promising candidate for bone defect repair	1947:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	10	89	theme	promising	1949:1957	arg1	granules					1929:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	9	90	theme	3D	1765:1766	arg1	angiography					1792:1802	3D microvascular perfusion angiography	1765:1802	3D microvascular perfusion angiography in terms of a higher vessel volume fraction	1765:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	8	91	theme	granules	1601:1608	arg1	inside					1587:1592	the inside	1583:1592	the inside of the granules	1583:1608	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	7	92	from	model	1330:1334	arg1	weeks					1261:1265	12 weeks	1258:1265	12 weeks of implantation in a critical-sized femoral bone defect animal model	1258:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	9	93	theme	perfusion	1782:1790	arg1	angiography					1792:1802	3D microvascular perfusion angiography	1765:1802	3D microvascular perfusion angiography in terms of a higher vessel volume fraction	1765:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	10	94	theme	HA	1911:1912	arg1	candidate					1959:1967	a promising candidate	1947:1967	a promising candidate for bone defect repair	1947:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	10	94	theme	HA	1911:1912	arg1	granules					1929:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	the core-shell structured HA@BCP bioceramic granules	1885:1936	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	5	95	theme	new	982:984	arg1	ingrowth					991:998	new bone ingrowth	982:998	new bone ingrowth	982:998	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	5	96	theme	%	889:889	arg1	porosity					872:879	a porosity	870:879	a porosity of 65-70%	870:889	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	4	97	theme	granules	697:704	arg1	structure					678:686	the porous structure	667:686	the porous structure	667:686	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	4	97	theme	granules	697:704	arg1	shape					657:661	The spherical shape	643:661	The spherical shape	643:661	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	2	98	theme	bone	372:375	arg1	filling					384:390	clinical bone defect filling	363:390	clinical bone defect filling due to their good osteoinductivity and biodegradability	363:446	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	0	99	theme	Structured	11:20	arg1	Spheres					58:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres	0:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.	0:96	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	1	100	theme	Adequate	98:105	arg1	challenge					163:171	a challenge	161:171	a challenge	161:171	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	1	100	theme	Adequate	98:105	arg1	regeneration					116:127	Adequate new bone regeneration	98:127	Adequate new bone regeneration in bone defects	98:143	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	0	101	theme	Calcium	29:35	arg1	Spheres					58:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres	0:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.	0:96	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	8	102	theme	bioceramic	1627:1636	arg1	phase					1638:1642	the bioceramic phase	1623:1642	the bioceramic phase	1623:1642	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	1	103	theme	bone	111:114	arg1	challenge					163:171	a challenge	161:171	a challenge	161:171	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	1	103	theme	bone	111:114	arg1	regeneration					116:127	Adequate new bone regeneration	98:127	Adequate new bone regeneration in bone defects	98:143	Adequate new bone regeneration in bone defects has always been a challenge as it requires excellent and efficient osteogenesis.
36251859	7	104	from	weeks	1261:1265	arg1	model					1330:1334	a critical-sized femoral bone defect animal model	1286:1334	a critical-sized femoral bone defect animal model	1286:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	2	105	theme	good	405:408	arg1	osteoinductivity					410:425	their good osteoinductivity	399:425	their good osteoinductivity	399:425	Calcium phosphate (CaP) bioceramics, including hydroxyapatite (HA) and biphasic calcium phosphates (BCPs), have been extensively used in clinical bone defect filling due to their good osteoinductivity and biodegradability.
36251859	9	106	theme	higher	1818:1823	arg1	fraction					1839:1846	a higher vessel volume fraction	1816:1846	a higher vessel volume fraction	1816:1846	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	4	107	theme	porous	671:676	arg1	structure					678:686	the porous structure	667:686	the porous structure	667:686	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	0	108	theme	Bioceramic	47:56	arg1	Spheres					58:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres	0:64	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.	0:96	Core-Shell Structured Porous Calcium Phosphate Bioceramic Spheres for Enhanced Bone Regeneration.
36251859	7	109	from	implantation	1270:1281	arg1	model					1330:1334	a critical-sized femoral bone defect animal model	1286:1334	a critical-sized femoral bone defect animal model	1286:1334	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	3	110	theme	BCP	611:613	arg1	HA					615:616	BCP@HA	611:616	BCP@HA	611:616	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	3	110	theme	BCP	611:613	arg1	shell					635:639	core@shell	630:639	core@shell	630:639	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	4	111	theme	alginate	735:742	arg1	technology					756:765	the calcium alginate gel molding technology	723:765	the calcium alginate gel molding technology combined with a H2O2 foaming process	723:802	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	7	112	theme	@	1407:1407	arg1	group					1412:1416	the HA@BCP group	1401:1416	the HA@BCP group	1401:1416	In vivo, 12 weeks of implantation in a critical-sized femoral bone defect animal model showed a higher bone volume fraction and bone mineral density in the HA@BCP group than in the BCP@HA or pure HA or BCP groups.
36251859	4	113	theme	molding	748:754	arg1	technology					756:765	the calcium alginate gel molding technology	723:765	the calcium alginate gel molding technology combined with a H2O2 foaming process	723:802	The spherical shape and the porous structure of these granules were achieved by the calcium alginate gel molding technology combined with a H2O2 foaming process.
36251859	9	114	from	region	1723:1728	arg1	vascularization					1693:1707	vascularization	1693:1707	vascularization at the defect region repaired by HA@BCP	1693:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	9	114	from	region	1723:1728	arg1	degree					1683:1688	A higher degree	1674:1688	A higher degree of vascularization at the defect region repaired by HA@BCP	1674:1747	A higher degree of vascularization at the defect region repaired by HA@BCP was revealed by 3D microvascular perfusion angiography in terms of a higher vessel volume fraction.
36251859	6	115	theme	In	1001:1002	arg1	experiments					1010:1020	In vitro experiments	1001:1020	In vitro experiments	1001:1020	In vitro experiments confirmed that HA@BCP bioceramic granules could promote the proliferation and osteogenic ability when cocultured with bone marrow mesenchymal stem cells, while inhibiting the differentiation of RAW264.7 cells into osteoclasts.
36251859	3	116	theme	HA	622:623	arg1	BCP					625:627	HA@BCP	622:627	HA@BCP	622:627	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	3	117	theme	first	463:467	arg1	time					469:472	the first time	459:472	the first time	459:472	Here, for the first time, we designed and fabricated two porous CaP bioceramic granules with core-shell structures, named in accordance with their composition as BCP@HA and HA@BCP (core@shell).
36251859	10	118	theme	bone	1973:1976	arg1	repair					1985:1990	bone defect repair	1973:1990	bone defect repair	1973:1990	The current study demonstrated that the core-shell structured HA@BCP bioceramic granules could be a promising candidate for bone defect repair.
36251859	5	119	theme	size	907:910	arg1	distribution					912:923	a micropore size distribution	895:923	a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth	895:998	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	5	120	with	distribution	912:923	arg1	porosity					872:879	a porosity	870:879	a porosity of 65-70%	870:889	These granules could be stacked to build a porous structure with a porosity of 65-70% and a micropore size distribution between 150 and 450 μm, which is reported to be good for new bone ingrowth.
36251859	8	121	theme	new	1514:1516	arg1	invading					1554:1561	invading	1554:1561	invading	1554:1561	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36251859	8	121	theme	new	1514:1516	arg1	tissue					1523:1528	the new bone tissue	1510:1528	the new bone tissue in the HA@BCP group	1510:1548	From histological analysis, we discovered that the new bone tissue in the HA@BCP group was invading from the surface to the inside of the granules, and most of the bioceramic phase was replaced by the new bone.
36209664	0	0	theme	water	67:71	arg1	plant					83:87	a drinking water treatment plant	56:87	a drinking water treatment plant in Catalonia, Spain	56:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	11	1	theme	MPs	1602:1604	arg1	intake					1606:1611	MPs intake	1602:1611	MPs intake from drinking water from this DWTP	1602:1646	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	11	1	theme	MPs	1602:1604	arg1	route					1669:1673	an important route	1656:1673	an important route compared to fish and seafood ingestion	1656:1712	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	4	2	theme	volumes	599:605	arg1	sampling					581:588	sampling	581:588	sampling of large volumes of water avoiding sample contamination	581:644	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	10	3	theme	different	1362:1370	arg1	types					1380:1384	Twenty-two different polymer types	1351:1384	Twenty-two different polymer types	1351:1384	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	11	4	theme	seafood	1696:1702	arg1	ingestion					1704:1712	seafood ingestion	1696:1712	seafood ingestion	1696:1712	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	0	5	theme	drinking	58:65	arg1	plant					83:87	a drinking water treatment plant	56:87	a drinking water treatment plant in Catalonia, Spain	56:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	10	6	theme	polymer	1372:1378	arg1	types					1380:1384	Twenty-two different polymer types	1351:1384	Twenty-two different polymer types	1351:1384	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	2	7	theme	MPs	320:322	arg1	composition					305:315	composition	305:315	composition	305:315	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	2	7	theme	MPs	320:322	arg1	concentration					274:286	concentration	274:286	concentration	274:286	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	2	7	theme	MPs	320:322	arg1	morphology					289:298	morphology	289:298	morphology	289:298	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	2	8	from	morphology	289:298	arg1	water					363:367	a drinking water	352:367	a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain)	352:439	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	1	9	theme	living	221:226	arg1	organisms					228:236	living organisms	221:236	living organisms	221:236	Microplastics (MPs) are emerging pollutants detected everywhere in the environment, with the potential to harm living organisms.
36209664	0	10	theme	plant	83:87	arg1	water					30:34	water	30:34	water	30:34	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	0	10	theme	plant	83:87	arg1	lines					47:51	sludge lines	40:51	sludge lines of a drinking water treatment plant in Catalonia, Spain	40:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	0	11	from	Screening	0:8	arg1	water					30:34	water	30:34	water	30:34	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	0	11	from	Screening	0:8	arg1	lines					47:51	sludge lines	40:51	sludge lines of a drinking water treatment plant in Catalonia, Spain	40:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	2	12	dep	Barcelona	409:417	arg1	Catalonia					420:428	Catalonia	420:428	Catalonia	420:428	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	2	12	dep	Barcelona	409:417	arg1	Spain					434:438	Spain	434:438	Spain	434:438	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	7	13	located	found	1073:1077	arg2	MPs					1064:1066	MPs	1064:1066	MPs	1064:1066	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	7	13	located	found	1073:1077	arg1	samples					1091:1097	all DWTP samples	1082:1097	all DWTP samples	1082:1097	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	3	14	theme	different	464:472	arg1	units					474:478	different units	464:478	different units of the DWTP	464:490	The sampling included different units of the DWTP, from influent to effluent as well as sludge line.
36209664	5	15	theme	chloride	892:899	arg1	solution					901:908	zinc chloride solution	887:908	zinc chloride solution	887:908	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	0	16	theme	treatment	73:81	arg1	plant					83:87	a drinking water treatment plant	56:87	a drinking water treatment plant in Catalonia, Spain	56:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	0	17	from	lines	47:51	arg1	Spain					103:107	Spain	103:107	Spain	103:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	6	18	theme	infrared	1020:1027	arg1	spectroscopic					1029:1041	Fourier-transform infrared spectroscopic	1002:1041	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	6	18	theme	infrared	1020:1027	arg1	FTIR					1044:1047	FTIR	1044:1047	FTIR	1044:1047	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	7	19	theme	plant	1202:1206	arg1	effluent					1186:1193	effluent	1186:1193	effluent	1186:1193	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	7	19	theme	plant	1202:1206	arg1	influent					1173:1180	influent	1173:1180	influent	1173:1180	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	4	20	theme	water	610:614	arg1	contamination					632:644	water avoiding sample contamination	610:644	water avoiding sample contamination	610:644	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	10	21	theme	synthetic	1406:1414	arg1	polypropylene					1449:1461	polypropylene	1449:1461	polypropylene	1449:1461	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	10	21	theme	synthetic	1406:1414	arg1	polyacrylonitrile					1496:1512	polyacrylonitrile	1496:1512	polyacrylonitrile	1496:1512	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	10	21	theme	synthetic	1406:1414	arg1	cellulose					1416:1424	cellulose	1416:1424	cellulose	1416:1424	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	10	21	theme	synthetic	1406:1414	arg1	polyester					1427:1435	polyester	1427:1435	polyester	1427:1435	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	10	21	theme	synthetic	1406:1414	arg1	polyethylene					1464:1475	polyethylene	1464:1475	polyethylene	1464:1475	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	10	21	theme	synthetic	1406:1414	arg1	polyurethane					1478:1489	polyurethane	1478:1489	polyurethane	1478:1489	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	10	21	theme	synthetic	1406:1414	arg1	common					1528:1533	common	1528:1533	common	1528:1533	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	10	21	theme	synthetic	1406:1414	arg1	polyamide					1438:1446	polyamide	1438:1446	polyamide	1438:1446	Twenty-two different polymer types were identified and synthetic cellulose, polyester, polyamide, polypropylene, polyethylene, polyurethane, and polyacrylonitrile were the most common.
36209664	2	22	from	composition	305:315	arg1	water					363:367	a drinking water	352:367	a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain)	352:439	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	2	23	theme	present	243:249	arg1	study					251:255	The present study	239:255	The present study	239:255	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	1	24	theme	emerging	134:141	arg1	Microplastics					110:122	Microplastics	110:122	Microplastics (MPs)	110:128	Microplastics (MPs) are emerging pollutants detected everywhere in the environment, with the potential to harm living organisms.
36209664	1	24	theme	emerging	134:141	arg1	pollutants					143:152	emerging pollutants	134:152	emerging pollutants detected everywhere in the environment, with the potential to harm living organisms	134:236	Microplastics (MPs) are emerging pollutants detected everywhere in the environment, with the potential to harm living organisms.
36209664	2	25	theme	located	392:398	arg1	water					363:367	a drinking water	352:367	a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain)	352:439	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	6	26	theme	Fourier-transform	1002:1018	arg1	spectroscopic					1029:1041	Fourier-transform infrared spectroscopic	1002:1041	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	6	26	theme	Fourier-transform	1002:1018	arg1	FTIR					1044:1047	FTIR	1044:1047	FTIR	1044:1047	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	0	27	from	water	30:34	arg1	Spain					103:107	Spain	103:107	Spain	103:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	3	28	theme	DWTP	487:490	arg1	units					474:478	different units	464:478	different units of the DWTP	464:490	The sampling included different units of the DWTP, from influent to effluent as well as sludge line.
36209664	5	29	theme	advanced	770:777	arg1	oxidation					779:787	advanced oxidation	770:787	advanced oxidation	770:787	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	4	30	theme	contamination	632:644	arg1	volumes					599:605	large volumes	593:605	large volumes of water avoiding sample contamination	593:644	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	3	31	dep	effluent	510:517	arg1	influent					498:505	influent	498:505	influent	498:505	The sampling included different units of the DWTP, from influent to effluent as well as sludge line.
36209664	8	32	theme	overall	1227:1233	arg1	%					1275:1275	98.3%	1271:1275	98.3%	1271:1275	The overall removal efficiency of the plant was 98.3%.
36209664	8	32	theme	overall	1227:1233	arg1	efficiency					1243:1252	The overall removal efficiency	1223:1252	The overall removal efficiency of the plant	1223:1265	The overall removal efficiency of the plant was 98.3%.
36209664	5	33	theme	density	846:852	arg1	separation					854:863	density separation	846:863	density separation of the particles with zinc chloride solution	846:908	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	0	34	theme	microplastics	13:25	arg1	Screening					0:8	Screening	0:8	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.	0:108	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	5	35	theme	particles	872:880	arg1	separation					854:863	density separation	846:863	density separation of the particles with zinc chloride solution	846:908	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	6	36	with	microscope	980:989	arg1	confirmation					1050:1061	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	4	37	theme	large	593:597	arg1	volumes					599:605	large volumes	593:605	large volumes of water avoiding sample contamination	593:644	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	6	38	theme	optical	955:961	arg1	microscope					980:989	an optical and stereoscopic microscope	952:989	an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation	952:1061	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	5	39	with	particles	872:880	arg1	solution					901:908	zinc chloride solution	887:908	zinc chloride solution	887:908	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	6	40	theme	stereoscopic	967:978	arg1	microscope					980:989	an optical and stereoscopic microscope	952:989	an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation	952:1061	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	2	41	theme	drinking	354:361	arg1	water					363:367	a drinking water	352:367	a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain)	352:439	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	6	42	theme	final	996:1000	arg1	confirmation					1050:1061	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	11	43	theme	drinking	1618:1625	arg1	water					1627:1631	drinking water	1618:1631	drinking water from this DWTP	1618:1646	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	4	44	theme	MPs	709:711	arg1	representativeness					687:704	the representativeness	683:704	the representativeness of MPs collected	683:721	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	4	45	theme	Sampling	543:550	arg1	filtration					562:571	filtration	562:571	filtration	562:571	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	4	45	theme	Sampling	543:550	arg1	strategy					552:559	Sampling strategy	543:559	Sampling strategy	543:559	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	11	46	from	water	1627:1631	arg1	intake					1606:1611	MPs intake	1602:1611	MPs intake from drinking water from this DWTP	1602:1646	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	11	46	from	water	1627:1631	arg1	route					1669:1673	an important route	1656:1673	an important route compared to fish and seafood ingestion	1656:1712	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	7	47	from	4.23 ± 1.26 MPs/L	1125:1141	arg1	concentrations					1105:1118	concentrations	1105:1118	concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively	1105:1220	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	5	48	dep	consisted	757:765	arg1	followed					834:841	followed	834:841	followed by density separation of the particles with zinc chloride solution	834:908	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	11	49	theme	important	1659:1667	arg1	intake					1606:1611	MPs intake	1602:1611	MPs intake from drinking water from this DWTP	1602:1646	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	11	49	theme	important	1659:1667	arg1	route					1669:1673	an important route	1656:1673	an important route compared to fish and seafood ingestion	1656:1712	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	2	50	dep	concentration	274:286	arg1	the					270:272	the	270:272	the	270:272	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	0	51	theme	sludge	40:45	arg1	lines					47:51	sludge lines	40:51	sludge lines of a drinking water treatment plant in Catalonia, Spain	40:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	9	52	theme	dominant	1287:1294	arg1	fibers					1311:1316	fibers	1311:1316	fibers followed by fragments and films	1311:1348	The most dominant morphology was fibers followed by fragments and films.
36209664	9	52	theme	dominant	1287:1294	arg1	morphology					1296:1305	The most dominant morphology	1278:1305	The most dominant morphology	1278:1305	The most dominant morphology was fibers followed by fragments and films.
36209664	5	53	theme	hydrogen	815:822	arg1	peroxide					824:831	hydrogen peroxide	815:831	hydrogen peroxide	815:831	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	7	54	from	0.075 ± 0.019 MPs/L	1146:1164	arg1	effluent					1186:1193	effluent	1186:1193	effluent	1186:1193	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	7	54	from	0.075 ± 0.019 MPs/L	1146:1164	arg1	influent					1173:1180	influent	1173:1180	influent	1173:1180	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	5	55	theme	zinc	887:890	arg1	solution					901:908	zinc chloride solution	887:908	zinc chloride solution	887:908	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	4	56	theme	sample	625:630	arg1	contamination					632:644	water avoiding sample contamination	610:644	water avoiding sample contamination	610:644	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	0	57	from	plant	83:87	arg1	Spain					103:107	Spain	103:107	Spain	103:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	3	58	theme	sludge	530:535	arg1	line					537:540	sludge line	530:540	effluent as well as sludge line	510:540	The sampling included different units of the DWTP, from influent to effluent as well as sludge line.
36209664	4	59	theme	avoiding	616:623	arg1	contamination					632:644	water avoiding sample contamination	610:644	water avoiding sample contamination	610:644	Sampling strategy, filtration, allows sampling of large volumes of water avoiding sample contamination, and during 8 h in order to increase the representativeness of MPs collected.
36209664	8	60	theme	plant	1261:1265	arg1	%					1275:1275	98.3%	1271:1275	98.3%	1271:1275	The overall removal efficiency of the plant was 98.3%.
36209664	8	60	theme	plant	1261:1265	arg1	efficiency					1243:1252	The overall removal efficiency	1223:1252	The overall removal efficiency of the plant	1223:1265	The overall removal efficiency of the plant was 98.3%.
36209664	8	61	theme	removal	1235:1241	arg1	%					1275:1275	98.3%	1271:1275	98.3%	1271:1275	The overall removal efficiency of the plant was 98.3%.
36209664	8	61	theme	removal	1235:1241	arg1	efficiency					1243:1252	The overall removal efficiency	1223:1252	The overall removal efficiency of the plant	1223:1265	The overall removal efficiency of the plant was 98.3%.
36209664	11	62	from	DWTP	1643:1646	arg1	water					1627:1631	drinking water	1618:1631	drinking water from this DWTP	1618:1646	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	5	63	theme	samples	749:755	arg1	pre-treatment					728:740	The pre-treatment	724:740	The pre-treatment of the samples	724:755	The pre-treatment of the samples consisted of advanced oxidation with Fenton's reagent and hydrogen peroxide, followed by density separation of the particles with zinc chloride solution.
36209664	7	64	dep	influent	1173:1180	arg1	the					1169:1171	the	1169:1171	the	1169:1171	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	0	65	from	Spain	103:107	arg1	water					30:34	water	30:34	water	30:34	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	0	65	from	Spain	103:107	arg1	lines					47:51	sludge lines	40:51	sludge lines of a drinking water treatment plant in Catalonia, Spain	40:107	Screening of microplastics in water and sludge lines of a drinking water treatment plant in Catalonia, Spain.
36209664	11	66	theme	distribution	1580:1591	arg1	network					1593:1599	the distribution network	1576:1599	the distribution network	1576:1599	Although MPs could be incorporated from the distribution network, MPs intake from drinking water from this DWTP was not an important route compared to fish and seafood ingestion.
36209664	7	67	theme	DWTP	1086:1089	arg1	samples					1091:1097	all DWTP samples	1082:1097	all DWTP samples	1082:1097	MPs were found in all DWTP samples, with concentrations from 4.23 ± 1.26 MPs/L to 0.075 ± 0.019 MPs/L in the influent and effluent of the plant, respectively.
36209664	6	68	theme	spectroscopic	1029:1041	arg1	confirmation					1050:1061	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	final Fourier-transform infrared spectroscopic (FTIR) confirmation	996:1061	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36209664	2	69	from	concentration	274:286	arg1	water					363:367	a drinking water	352:367	a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain)	352:439	The present study investigated the concentration, morphology, and composition of MPs, between 20 μm and 5 mm, in a drinking water treatment plant (DWTP) located close to Barcelona (Catalonia, NE Spain).
36209664	6	70	theme	Visual	911:916	arg1	identification					918:931	Visual identification	911:931	Visual identification	911:931	Visual identification was performed with an optical and stereoscopic microscope with final Fourier-transform infrared spectroscopic (FTIR) confirmation.
36384833	15	0	theme	stearic	2507:2513	arg1	acid					2515:2518	stearic acid	2507:2518	stearic acid	2507:2518	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	6	1	dep	sugar	816:820	arg1	The					804:806	The	804:806	The	804:806	The soluble sugar and starch in mature grains increased with altitudes, while soluble protein and crude fat did not change.
36384833	7	2	theme	phosphate	1006:1014	arg1	SPS					1026:1028	SPS	1026:1028	SPS	1026:1028	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	7	2	theme	phosphate	1006:1014	arg1	synthase					1016:1023	sucrose phosphate synthase	998:1023	sucrose phosphate synthase (SPS)	998:1029	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	16	3	theme	relative	2842:2849	arg1	increment					2851:2859	the relative increment	2838:2859	the relative increment	2838:2859	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	6	4	theme	soluble	808:814	arg1	sugar					816:820	soluble sugar	808:820	soluble sugar	808:820	The soluble sugar and starch in mature grains increased with altitudes, while soluble protein and crude fat did not change.
36384833	2	5	theme	expression	218:227	arg1	analysis					229:236	the expression analysis	214:236	the expression analysis of key genes	214:249	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	2	6	theme	Paeonia	279:285	arg1	cv					300:301	Paeonia suffruticosa cv	279:301	Paeonia suffruticosa cv	279:301	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	16	7	theme	relative	2762:2769	arg1	expression					2771:2780	The relative expression	2758:2780	The relative expression of ACCase, SAD, and FAD2	2758:2805	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	11	8	theme	sugar	1727:1731	arg1	contents					1707:1714	the contents	1703:1714	the contents of soluble sugar and starch	1703:1742	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	4	9	from	traits	433:438	arg1	metabolism					617:626	oil metabolism	613:626	oil metabolism	613:626	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	10	theme	genes	604:608	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	10	theme	genes	604:608	arg1	transformation					448:461	the transformation	444:461	the transformation of soluble sugar, starch, soluble protein and fatty acid contents	444:527	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	10	theme	genes	604:608	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	10	theme	genes	604:608	arg1	expression					586:595	differential expression	573:595	differential expression	573:595	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	7	11	theme	synthase	980:987	arg1	activities					958:967	the activities	954:967	the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS)	954:1029	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	11	12	theme	fat	1818:1820	arg1	contents					1780:1787	the contents	1776:1787	the contents of soluble protein and crude fat during grain development	1776:1845	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	15	13	theme	Palmitate	2491:2499	arg1	acid					2501:2504	Palmitate acid	2491:2504	Palmitate acid	2491:2504	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	4	14	from	differences	406:416	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	14	from	differences	406:416	arg1	transformation					448:461	the transformation	444:461	the transformation of soluble sugar, starch, soluble protein and fatty acid contents	444:527	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	14	from	differences	406:416	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	2	15	theme	cv	300:301	arg1	development					264:274	grain development	258:274	grain development of Paeonia suffruticosa cv	258:301	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	4	16	from	activity	560:567	arg1	metabolism					617:626	oil metabolism	613:626	oil metabolism	613:626	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	9	17	theme	oil	1497:1499	arg1	grain					1512:1516	oil tree peony grain	1497:1516	oil tree peony grain at different altitudes	1497:1539	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	15	18	theme	desaturation	2641:2652	arg1	process					2654:2660	fatty acid desaturation process	2630:2660	fatty acid desaturation process in the grain development of tree peony	2630:2699	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	4	19	theme	differential	573:584	arg1	expression					586:595	differential expression	573:595	differential expression	573:595	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	15	20	theme	linoleic	2524:2531	arg1	acid					2533:2536	linoleic acid	2524:2536	linoleic acid	2524:2536	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	16	21	theme	important	2911:2919	arg1	role					2921:2924	an important role	2908:2924	an important role	2908:2924	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	13	22	theme	PDH	2076:2078	arg1	Activity					2064:2071	Activity	2064:2071	Activity of PDH	2064:2078	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	9	23	theme	peony	1506:1510	arg1	grain					1512:1516	oil tree peony grain	1497:1516	oil tree peony grain at different altitudes	1497:1539	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	15	24	theme	fatty	2630:2634	arg1	acid					2636:2639	fatty acid	2630:2639	fatty acid desaturation process in the grain development of tree peony	2630:2699	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	10	25	theme	soluble	1578:1584	arg1	sugar					1586:1590	soluble sugar	1578:1590	soluble sugar	1578:1590	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	5	26	from	altitudes	747:755	arg1	period					717:722	the growth period	706:722	the growth period of grain at the higher altitudes	706:755	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	5	26	from	altitudes	747:755	arg1	longer					761:766	longer	761:766	longer	761:766	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	9	27	theme	acetyl-CoA	1341:1350	arg1	carboxylase					1352:1362	acetyl-CoA carboxylase	1341:1362	acetyl-CoA carboxylase (ACCase)	1341:1371	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	9	27	theme	acetyl-CoA	1341:1350	arg1	ACCase					1365:1370	ACCase	1365:1370	ACCase	1365:1370	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	12	28	with	correlation	2000:2010	arg1	contents					2021:2028	the contents	2017:2028	the contents of soluble protein and crude fat	2017:2061	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	13	29	theme	soluble	2221:2227	arg1	sugar					2229:2233	soluble sugar	2221:2233	soluble sugar	2221:2233	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	16	30	theme	acid	2941:2944	arg1	synthesis					2946:2954	α-linolenic acid synthesis	2929:2954	α-linolenic acid synthesis	2929:2954	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	1	31	theme	different	127:135	arg1	altitudes					137:145	different altitudes	127:145	different altitudes	127:145	'Fengdan' grown at different altitudes.]
36384833	4	32	theme	study	385:389	arg1	aim					373:375	The aim	369:375	The aim of this study	369:389	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	14	33	theme	important	2459:2467	arg1	role					2469:2472	an important role	2456:2472	an important role	2456:2472	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	8	34	theme	glutamic-pyruvic	1165:1180	arg1	GPT					1196:1198	GPT	1196:1198	GPT	1196:1198	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	8	34	theme	glutamic-pyruvic	1165:1180	arg1	transaminase					1182:1193	glutamic-pyruvic transaminase	1165:1193	glutamic-pyruvic transaminase (GPT)	1165:1199	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	15	35	theme	acid	2608:2611	arg1	increment					2583:2591	the relative increment	2570:2591	the relative increment of α-linolenic acid	2570:2611	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	5	36	theme	higher	740:745	arg1	altitudes					747:755	the higher altitudes	736:755	the higher altitudes	736:755	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	14	37	theme	tree	2326:2329	arg1	peony					2331:2335	tree peony	2326:2335	tree peony	2326:2335	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	12	38	theme	protein	2041:2047	arg1	contents					2021:2028	the contents	2017:2028	the contents of soluble protein and crude fat	2017:2061	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	0	39	theme	Paeonia	83:89	arg1	cv					104:105	Paeonia suffruticosa cv	83:105	Paeonia suffruticosa cv	83:105	[Grain oil quality formation and metabolism-related genes difference expression of Paeonia suffruticosa cv.
36384833	18	40	from	use	3183:3185	arg1	Luoyang					3207:3213	Luoyang	3207:3213	Luoyang	3207:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	9	41	theme	stearoyl-ACP	1377:1388	arg1	SAD					1402:1404	SAD	1402:1404	SAD	1402:1404	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	9	41	theme	stearoyl-ACP	1377:1388	arg1	desaturase					1390:1399	stearoyl-ACP desaturase	1377:1399	stearoyl-ACP desaturase (SAD)	1377:1405	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	14	42	theme	development	2311:2321	arg1	process					2294:2300	the process	2290:2300	the process of grain development of tree peony	2290:2335	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	12	43	theme	crude	2053:2057	arg1	fat					2059:2061	crude fat	2053:2061	crude fat	2053:2061	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	17	44	theme	grain	3043:3047	arg1	production					3049:3058	grain production	3043:3058	grain production	3043:3058	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	0	45	theme	cv	104:105	arg1	expression					69:78	expression	69:78	expression of Paeonia suffruticosa cv	69:105	[Grain oil quality formation and metabolism-related genes difference expression of Paeonia suffruticosa cv.
36384833	11	46	theme	soluble	1792:1798	arg1	protein					1800:1806	soluble protein	1792:1806	soluble protein	1792:1806	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	16	47	theme	acid	2876:2879	arg1	accumulation					2881:2892	α-linolenic acid accumulation	2864:2892	α-linolenic acid accumulation	2864:2892	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	6	48	theme	soluble	882:888	arg1	protein					890:896	soluble protein	882:896	soluble protein	882:896	The soluble sugar and starch in mature grains increased with altitudes, while soluble protein and crude fat did not change.
36384833	4	49	theme	grain	421:425	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	8	50	theme	glutamic-oxalacetic	1205:1223	arg1	GOT					1240:1242	GOT	1240:1242	GOT	1240:1242	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	8	50	theme	glutamic-oxalacetic	1205:1223	arg1	transaminease					1225:1237	glutamic-oxalacetic transaminease	1205:1237	glutamic-oxalacetic transaminease (GOT)	1205:1243	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	17	51	theme	oil	2961:2963	arg1	quality					2965:2971	The oil quality	2957:2971	The oil quality of tree peony grain	2957:2991	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	17	51	theme	oil	2961:2963	arg1	stable					3008:3013	stable	3008:3013	stable	3008:3013	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	3	52	theme	m	354:354	arg1	altitudes					323:331	altitudes	323:331	altitudes of 100, 650 and 1010 m	323:354	'Fengdan' grown at altitudes of 100, 650 and 1010 m in Luo-yang.
36384833	0	53	theme	[Grain	0:5	arg1	formation					19:27	[Grain oil quality formation	0:27	[Grain oil quality formation	0:27	[Grain oil quality formation and metabolism-related genes difference expression of Paeonia suffruticosa cv.
36384833	2	54	theme	key	241:243	arg1	genes					245:249	key genes	241:249	key genes	241:249	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	15	55	theme	tree	2690:2693	arg1	peony					2695:2699	tree peony	2690:2699	tree peony	2690:2699	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	4	56	theme	soluble	489:495	arg1	protein					497:503	soluble protein	489:503	soluble protein	489:503	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	17	57	theme	peony	2981:2985	arg1	grain					2987:2991	tree peony grain	2976:2991	tree peony grain	2976:2991	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	0	58	theme	quality	11:17	arg1	formation					19:27	[Grain oil quality formation	0:27	[Grain oil quality formation	0:27	[Grain oil quality formation and metabolism-related genes difference expression of Paeonia suffruticosa cv.
36384833	15	59	theme	relative	2574:2581	arg1	increment					2583:2591	the relative increment	2570:2591	the relative increment of α-linolenic acid	2570:2611	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	4	60	theme	oil	613:615	arg1	metabolism					617:626	oil metabolism	613:626	oil metabolism	613:626	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	18	61	theme	tree	3098:3101	arg1	strategy					3156:3163	an important strategy	3143:3163	an important strategy for the efficient use of marginal land in Luoyang	3143:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	18	61	theme	tree	3098:3101	arg1	peony					3103:3107	Planting oil tree peony	3085:3107	Planting oil tree peony at mid to high altitudes	3085:3132	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	2	62	theme	morphological	165:177	arg1	index					179:183	the morphological index	161:183	the morphological index	161:183	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	17	63	theme	different	3018:3026	arg1	altitudes					3028:3036	different altitudes	3018:3036	different altitudes	3018:3036	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	5	64	theme	growth	710:715	arg1	period					717:722	the growth period	706:722	the growth period of grain at the higher altitudes	706:755	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	5	64	theme	growth	710:715	arg1	longer					761:766	longer	761:766	longer	761:766	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	18	65	theme	Planting	3085:3092	arg1	strategy					3156:3163	an important strategy	3143:3163	an important strategy for the efficient use of marginal land in Luoyang	3143:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	18	65	theme	Planting	3085:3092	arg1	peony					3103:3107	Planting oil tree peony	3085:3107	Planting oil tree peony at mid to high altitudes	3085:3132	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	4	66	theme	sugar	474:478	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	66	theme	sugar	474:478	arg1	transformation					448:461	the transformation	444:461	the transformation of soluble sugar, starch, soluble protein and fatty acid contents	444:527	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	66	theme	sugar	474:478	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	66	theme	sugar	474:478	arg1	expression					586:595	differential expression	573:595	differential expression	573:595	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	10	67	theme	soluble	1628:1634	arg1	protein					1636:1642	soluble protein	1628:1642	soluble protein	1628:1642	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	14	68	theme	nutrient	2265:2272	arg1	accumulation					2274:2285	nutrient accumulation	2265:2285	nutrient accumulation in the process of grain development of tree peony	2265:2335	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	4	69	theme	starch	481:486	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	69	theme	starch	481:486	arg1	transformation					448:461	the transformation	444:461	the transformation of soluble sugar, starch, soluble protein and fatty acid contents	444:527	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	69	theme	starch	481:486	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	69	theme	starch	481:486	arg1	expression					586:595	differential expression	573:595	differential expression	573:595	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	12	70	theme	sugar	1929:1933	arg1	content					1910:1916	the content	1906:1916	the content of soluble sugar	1906:1933	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	12	70	theme	sugar	1929:1933	arg1	content					1943:1949	the content	1939:1949	the content of starch	1939:1959	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	9	71	from	altitudes	1531:1539	arg1	grain					1512:1516	oil tree peony grain	1497:1516	oil tree peony grain at different altitudes	1497:1539	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	9	72	theme	fatty	1447:1451	arg1	FAD2					1472:1475	FAD2	1472:1475	FAD2	1472:1475	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	9	72	theme	fatty	1447:1451	arg1	desaturase					1458:1467	ω-6 fatty acid desaturase 2	1443:1469	ω-6 fatty acid desaturase 2 (FAD2)	1443:1476	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	15	73	theme	acid	2742:2745	arg1	synthesis					2747:2755	α-linolenic acid synthesis	2730:2755	α-linolenic acid synthesis	2730:2755	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	17	74	from	altitudes	3028:3036	arg1	quality					2965:2971	The oil quality	2957:2971	The oil quality of tree peony grain	2957:2991	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	17	74	from	altitudes	3028:3036	arg1	stable					3008:3013	stable	3008:3013	stable	3008:3013	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	6	75	from	sugar	816:820	arg1	grains					843:848	mature grains	836:848	mature grains	836:848	The soluble sugar and starch in mature grains increased with altitudes, while soluble protein and crude fat did not change.
36384833	10	76	theme	fat	1654:1656	arg1	accumulation					1612:1623	the accumulation	1608:1623	the accumulation of soluble protein and crude fat	1608:1656	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	11	77	theme	SPS	1659:1661	arg1	activity					1663:1670	SPS activity	1659:1670	SPS activity	1659:1670	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	13	78	theme	soluble	2126:2132	arg1	proteins					2134:2141	soluble proteins	2126:2141	soluble proteins	2126:2141	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	14	79	theme	metabolic	2399:2407	arg1	GOT					2444:2446	GOT	2444:2446	GOT	2444:2446	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	14	79	theme	metabolic	2399:2407	arg1	enzymes					2409:2415	metabolic enzymes	2399:2415	metabolic enzymes	2399:2415	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	14	79	theme	metabolic	2399:2407	arg1	PDH					2431:2433	PDH	2431:2433	PDH	2431:2433	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	14	79	theme	metabolic	2399:2407	arg1	GPT					2436:2438	GPT	2436:2438	GPT	2436:2438	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	14	79	theme	metabolic	2399:2407	arg1	SPS					2426:2428	SPS	2426:2428	SPS	2426:2428	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	6	80	from	starch	826:831	arg1	grains					843:848	mature grains	836:848	mature grains	836:848	The soluble sugar and starch in mature grains increased with altitudes, while soluble protein and crude fat did not change.
36384833	7	81	theme	sucrose	998:1004	arg1	SPS					1026:1028	SPS	1026:1028	SPS	1026:1028	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	7	81	theme	sucrose	998:1004	arg1	synthase					1016:1023	sucrose phosphate synthase	998:1023	sucrose phosphate synthase (SPS)	998:1029	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	18	82	from	mid	3112:3114	arg1	strategy					3156:3163	an important strategy	3143:3163	an important strategy for the efficient use of marginal land in Luoyang	3143:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	18	82	from	mid	3112:3114	arg1	peony					3103:3107	Planting oil tree peony	3085:3107	Planting oil tree peony at mid to high altitudes	3085:3132	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	4	83	theme	acid	515:518	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	83	theme	acid	515:518	arg1	transformation					448:461	the transformation	444:461	the transformation of soluble sugar, starch, soluble protein and fatty acid contents	444:527	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	83	theme	acid	515:518	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	83	theme	acid	515:518	arg1	expression					586:595	differential expression	573:595	differential expression	573:595	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	84	from	transformation	448:461	arg1	metabolism					617:626	oil metabolism	613:626	oil metabolism	613:626	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	2	85	theme	suffruticosa	287:298	arg1	cv					300:301	Paeonia suffruticosa cv	279:301	Paeonia suffruticosa cv	279:301	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	15	86	theme	α-linolenic	2596:2606	arg1	acid					2608:2611	α-linolenic acid	2596:2611	α-linolenic acid	2596:2611	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	18	87	theme	important	3146:3154	arg1	strategy					3156:3163	an important strategy	3143:3163	an important strategy for the efficient use of marginal land in Luoyang	3143:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	18	87	theme	important	3146:3154	arg1	peony					3103:3107	Planting oil tree peony	3085:3107	Planting oil tree peony at mid to high altitudes	3085:3132	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	7	88	theme	sucrose	972:978	arg1	SS					990:991	SS	990:991	SS	990:991	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	7	88	theme	sucrose	972:978	arg1	synthase					980:987	sucrose synthase	972:987	sucrose synthase (SS)	972:992	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	11	89	theme	crude	1812:1816	arg1	fat					1818:1820	crude fat	1812:1820	crude fat during grain development	1812:1845	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	4	90	from	expression	586:595	arg1	metabolism					617:626	oil metabolism	613:626	oil metabolism	613:626	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	5	91	theme	low	781:783	arg1	altitudes					793:801	low and mid altitudes	781:801	low and mid altitudes	781:801	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	12	92	theme	fat	2059:2061	arg1	contents					2021:2028	the contents	2017:2028	the contents of soluble protein and crude fat	2017:2061	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	4	93	theme	key	600:602	arg1	genes					604:608	key genes	600:608	key genes	600:608	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	13	94	theme	sugar	2229:2233	arg1	contents					2209:2216	the contents	2205:2216	the contents of soluble sugar and starch	2205:2244	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	10	95	theme	negative	1554:1561	arg1	correlation					1563:1573	a negative correlation	1552:1573	a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat	1552:1656	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	16	96	theme	ACCase	2785:2790	arg1	expression					2771:2780	The relative expression	2758:2780	The relative expression of ACCase, SAD, and FAD2	2758:2805	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	12	97	dep	significant	1979:1989	arg1	positive					1991:1998	positive	1991:1998	positive	1991:1998	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	18	98	theme	efficient	3173:3181	arg1	use					3183:3185	the efficient use	3169:3185	the efficient use of marginal land in Luoyang	3169:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	4	99	theme	enzyme	553:558	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	13	100	theme	starch	2239:2244	arg1	contents					2209:2216	the contents	2205:2216	the contents of soluble sugar and starch	2205:2244	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	10	101	theme	starch	1596:1601	arg1	correlation					1563:1573	a negative correlation	1552:1573	a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat	1552:1656	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	16	102	theme	SAD	2793:2795	arg1	expression					2771:2780	The relative expression	2758:2780	The relative expression of ACCase, SAD, and FAD2	2758:2805	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	9	103	theme	relative	1318:1325	arg1	expression					1327:1336	The relative expression	1314:1336	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD)	1314:1405	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	14	104	theme	crude	2367:2371	arg1	fat					2373:2375	crude fat	2367:2375	crude fat	2367:2375	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	9	105	theme	tree	1501:1504	arg1	grain					1512:1516	oil tree peony grain	1497:1516	oil tree peony grain at different altitudes	1497:1539	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	7	106	theme	grain	935:939	arg1	development					941:951	grain development	935:951	grain development	935:951	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	10	107	theme	sugar	1586:1590	arg1	correlation					1563:1573	a negative correlation	1552:1573	a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat	1552:1656	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	6	108	theme	mature	836:841	arg1	grains					843:848	mature grains	836:848	mature grains	836:848	The soluble sugar and starch in mature grains increased with altitudes, while soluble protein and crude fat did not change.
36384833	15	109	theme	acid	2636:2639	arg1	process					2654:2660	fatty acid desaturation process	2630:2660	fatty acid desaturation process in the grain development of tree peony	2630:2699	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	8	110	theme	dehydrogenase	1144:1156	arg1	activities					1121:1130	The activities	1117:1130	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT)	1117:1243	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	2	111	theme	grain	258:262	arg1	development					264:274	grain development	258:274	grain development of Paeonia suffruticosa cv	258:301	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	9	112	theme	carboxylase	1352:1362	arg1	expression					1327:1336	The relative expression	1314:1336	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD)	1314:1405	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	11	113	theme	protein	1800:1806	arg1	contents					1780:1787	the contents	1776:1787	the contents of soluble protein and crude fat during grain development	1776:1845	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	9	114	theme	different	1521:1529	arg1	altitudes					1531:1539	different altitudes	1521:1539	different altitudes	1521:1539	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	12	115	theme	GPT	1862:1864	arg1	Activities					1848:1857	Activities	1848:1857	Activities of GPT and GOT	1848:1872	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	2	116	theme	genes	245:249	arg1	analysis					229:236	the expression analysis	214:236	the expression analysis of key genes	214:249	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	2	116	theme	genes	245:249	arg1	index					179:183	the morphological index	161:183	the morphological index	161:183	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	2	116	theme	genes	245:249	arg1	composition					198:208	nutritional composition	186:208	nutritional composition	186:208	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	16	117	theme	α-linolenic	2929:2939	arg1	synthesis					2946:2954	α-linolenic acid synthesis	2929:2954	α-linolenic acid synthesis	2929:2954	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	5	118	theme	mid	789:791	arg1	altitudes					793:801	low and mid altitudes	781:801	low and mid altitudes	781:801	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	14	119	theme	peony	2331:2335	arg1	development					2311:2321	grain development	2305:2321	grain development of tree peony	2305:2335	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	5	120	theme	yield	659:663	arg1	traits					665:670	grain yield traits	653:670	grain yield traits	653:670	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	0	121	theme	suffruticosa	91:102	arg1	cv					104:105	Paeonia suffruticosa cv	83:105	Paeonia suffruticosa cv	83:105	[Grain oil quality formation and metabolism-related genes difference expression of Paeonia suffruticosa cv.
36384833	6	122	theme	crude	902:906	arg1	fat					908:910	crude fat	902:910	crude fat	902:910	The soluble sugar and starch in mature grains increased with altitudes, while soluble protein and crude fat did not change.
36384833	4	123	theme	soluble	466:472	arg1	sugar					474:478	soluble sugar	466:478	soluble sugar	466:478	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	7	124	dep	decreased	1037:1045	arg1	with					1067:1070	with	1067:1070	with	1067:1070	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	8	125	theme	transaminase	1182:1193	arg1	activities					1121:1130	The activities	1117:1130	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT)	1117:1243	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	5	126	theme	grain	653:657	arg1	traits					665:670	grain yield traits	653:670	grain yield traits	653:670	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	4	127	dep	sugar	474:478	arg1	contents					520:527	contents	520:527	contents	520:527	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	9	128	theme	desaturase	1390:1399	arg1	expression					1327:1336	The relative expression	1314:1336	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD)	1314:1405	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	14	129	theme	grain	2305:2309	arg1	development					2311:2321	grain development	2305:2321	grain development of tree peony	2305:2335	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	4	130	theme	yield	427:431	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	12	131	theme	significant	1979:1989	arg1	correlation					2000:2010	a highly significant positive correlation	1970:2010	a highly significant positive correlation with the contents of soluble protein and crude fat	1970:2061	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	12	132	theme	GOT	1870:1872	arg1	Activities					1848:1857	Activities	1848:1857	Activities of GPT and GOT	1848:1872	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	18	133	theme	land	3199:3202	arg1	use					3183:3185	the efficient use	3169:3185	the efficient use of marginal land in Luoyang	3169:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	15	134	theme	peony	2695:2699	arg1	development					2675:2685	the grain development	2665:2685	the grain development of tree peony	2665:2699	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	10	135	with	correlation	1563:1573	arg1	accumulation					1612:1623	the accumulation	1608:1623	the accumulation of soluble protein and crude fat	1608:1656	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	12	136	theme	soluble	2033:2039	arg1	protein					2041:2047	soluble protein	2033:2047	soluble protein	2033:2047	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	5	137	theme	grain	727:731	arg1	period					717:722	the growth period	706:722	the growth period of grain at the higher altitudes	706:755	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	5	137	theme	grain	727:731	arg1	longer					761:766	longer	761:766	longer	761:766	The results showed that grain yield traits increased with altitudes and that the growth period of grain at the higher altitudes was longer than that at low and mid altitudes.
36384833	16	138	theme	accumulation	2881:2892	arg1	increment					2851:2859	the relative increment	2838:2859	the relative increment	2838:2859	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	13	139	theme	GPT	2161:2163	arg1	activities					2147:2156	activities	2147:2156	activities of GPT and GOT	2147:2171	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	13	139	theme	GPT	2161:2163	arg1	content					2115:2121	the content	2111:2121	the content of soluble proteins	2111:2141	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	4	140	theme	protein	497:503	arg1	traits					433:438	grain yield traits	421:438	grain yield traits	421:438	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	140	theme	protein	497:503	arg1	transformation					448:461	the transformation	444:461	the transformation of soluble sugar, starch, soluble protein and fatty acid contents	444:527	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	140	theme	protein	497:503	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	4	140	theme	protein	497:503	arg1	expression					586:595	differential expression	573:595	differential expression	573:595	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	8	141	theme	transaminease	1225:1237	arg1	activities					1121:1130	The activities	1117:1130	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT)	1117:1243	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	17	142	theme	tree	2976:2979	arg1	grain					2987:2991	tree peony grain	2976:2991	tree peony grain	2976:2991	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	16	143	theme	α-linolenic	2864:2874	arg1	accumulation					2881:2892	α-linolenic acid accumulation	2864:2892	α-linolenic acid accumulation	2864:2892	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	13	144	theme	GOT	2169:2171	arg1	activities					2147:2156	activities	2147:2156	activities of GPT and GOT	2147:2171	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	13	144	theme	GOT	2169:2171	arg1	content					2115:2121	the content	2111:2121	the content of soluble proteins	2111:2141	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	11	145	theme	starch	1737:1742	arg1	contents					1707:1714	the contents	1703:1714	the contents of soluble sugar and starch	1703:1742	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	15	146	theme	grain	2669:2673	arg1	development					2675:2685	the grain development	2665:2685	the grain development of tree peony	2665:2699	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	4	147	theme	fatty	509:513	arg1	acid					515:518	fatty acid	509:518	fatty acid	509:518	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	14	148	from	accumulation	2274:2285	arg1	process					2294:2300	the process	2290:2300	the process of grain development of tree peony	2290:2335	It suggested that nutrient accumulation in the process of grain development of tree peony was transformed from sugar to crude fat and protein, and that metabolic enzymes, such as SPS, PDH, GPT and GOT, played an important role in this process.
36384833	10	149	theme	crude	1648:1652	arg1	fat					1654:1656	crude fat	1648:1656	crude fat	1648:1656	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	17	150	theme	grain	2987:2991	arg1	quality					2965:2971	The oil quality	2957:2971	The oil quality of tree peony grain	2957:2991	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	17	150	theme	grain	2987:2991	arg1	stable					3008:3013	stable	3008:3013	stable	3008:3013	The oil quality of tree peony grain was relatively stable at different altitudes, but grain production increased with altitude.
36384833	11	151	theme	soluble	1719:1725	arg1	sugar					1727:1731	soluble sugar	1719:1731	soluble sugar	1719:1731	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	0	152	theme	metabolism-related	33:50	arg1	genes					52:56	metabolism-related genes	33:56	metabolism-related genes	33:56	[Grain oil quality formation and metabolism-related genes difference expression of Paeonia suffruticosa cv.
36384833	8	153	theme	pyruvate	1135:1142	arg1	PDH					1159:1161	PDH	1159:1161	PDH	1159:1161	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	8	153	theme	pyruvate	1135:1142	arg1	dehydrogenase					1144:1156	pyruvate dehydrogenase	1135:1156	pyruvate dehydrogenase (PDH)	1135:1162	The activities of pyruvate dehydrogenase (PDH), glutamic-pyruvic transaminase (GPT) and glutamic-oxalacetic transaminease (GOT) increased rapidly during 50-90 d after flowering and peaked at 90 d.
36384833	13	154	theme	proteins	2134:2141	arg1	activities					2147:2156	activities	2147:2156	activities of GPT and GOT	2147:2171	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	13	154	theme	proteins	2134:2141	arg1	content					2115:2121	the content	2111:2121	the content of soluble proteins	2111:2141	Activity of PDH was positively correlated with the content of soluble proteins and activities of GPT and GOT, and negatively correlated with the contents of soluble sugar and starch.
36384833	15	155	from	process	2654:2660	arg1	development					2675:2685	the grain development	2665:2685	the grain development of tree peony	2665:2699	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	18	156	theme	oil	3094:3096	arg1	strategy					3156:3163	an important strategy	3143:3163	an important strategy for the efficient use of marginal land in Luoyang	3143:3213	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	18	156	theme	oil	3094:3096	arg1	peony					3103:3107	Planting oil tree peony	3085:3107	Planting oil tree peony at mid to high altitudes	3085:3132	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	10	157	theme	protein	1636:1642	arg1	accumulation					1612:1623	the accumulation	1608:1623	the accumulation of soluble protein and crude fat	1608:1656	There was a negative correlation of soluble sugar and starch with the accumulation of soluble protein and crude fat.
36384833	18	158	theme	high	3119:3122	arg1	altitudes					3124:3132	high altitudes	3119:3132	high altitudes	3119:3132	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	7	159	theme	synthase	1016:1023	arg1	activities					958:967	the activities	954:967	the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS)	954:1029	During grain development, the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) first decreased and then increased, with the lowest occurred at 90 d after flowering.
36384833	4	160	dep	activity	560:567	arg1	the					541:543	the	541:543	the	541:543	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	15	161	theme	synthesis	2747:2755	arg1	direction					2717:2725	the direction	2713:2725	the direction of α-linolenic acid synthesis	2713:2755	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	11	162	theme	grain	1829:1833	arg1	development					1835:1845	grain development	1829:1845	grain development	1829:1845	SPS activity was positively correlated with the contents of soluble sugar and starch, and negatively correlated with the contents of soluble protein and crude fat during grain development.
36384833	12	163	theme	starch	1954:1959	arg1	content					1910:1916	the content	1906:1916	the content of soluble sugar	1906:1933	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	12	163	theme	starch	1954:1959	arg1	content					1943:1949	the content	1939:1949	the content of starch	1939:1959	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	16	164	theme	FAD2	2802:2805	arg1	expression					2771:2780	The relative expression	2758:2780	The relative expression of ACCase, SAD, and FAD2	2758:2805	The relative expression of ACCase, SAD, and FAD2 was positively correlated with the relative increment of α-linolenic acid accumulation, which played an important role in α-linolenic acid synthesis.
36384833	9	165	theme	ω-6	1443:1445	arg1	FAD2					1472:1475	FAD2	1472:1475	FAD2	1472:1475	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	9	165	theme	ω-6	1443:1445	arg1	desaturase					1458:1467	ω-6 fatty acid desaturase 2	1443:1469	ω-6 fatty acid desaturase 2 (FAD2)	1443:1476	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	15	166	theme	α-linolenic	2730:2740	arg1	synthesis					2747:2755	α-linolenic acid synthesis	2730:2755	α-linolenic acid synthesis	2730:2755	Palmitate acid, stearic acid and linoleic acid were negatively correlated with the relative increment of α-linolenic acid, indicating that fatty acid desaturation process in the grain development of tree peony was towards the direction of α-linolenic acid synthesis.
36384833	2	167	theme	nutritional	186:196	arg1	composition					198:208	nutritional composition	186:208	nutritional composition	186:208	We measured the morphological index, nutritional composition and the expression analysis of key genes during grain development of Paeonia suffruticosa cv.
36384833	12	168	theme	soluble	1921:1927	arg1	sugar					1929:1933	soluble sugar	1921:1933	soluble sugar	1921:1933	Activities of GPT and GOT were negatively associated with the content of soluble sugar and the content of starch, and had a highly significant positive correlation with the contents of soluble protein and crude fat.
36384833	9	169	theme	acid	1453:1456	arg1	FAD2					1472:1475	FAD2	1472:1475	FAD2	1472:1475	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	9	169	theme	acid	1453:1456	arg1	desaturase					1458:1467	ω-6 fatty acid desaturase 2	1443:1469	ω-6 fatty acid desaturase 2 (FAD2)	1443:1476	The relative expression of acetyl-CoA carboxylase (ACCase) and stearoyl-ACP desaturase (SAD) peaked at 50 d after flowering, and ω-6 fatty acid desaturase 2 (FAD2) peaked at 90 d, in oil tree peony grain at different altitudes.
36384833	18	170	theme	marginal	3190:3197	arg1	land					3199:3202	marginal land	3190:3202	marginal land	3190:3202	Planting oil tree peony at mid to high altitudes could be an important strategy for the efficient use of marginal land in Luoyang.
36384833	4	171	theme	related	545:551	arg1	activity					560:567	related enzyme activity	545:567	related enzyme activity	545:567	The aim of this study was to examine differences in grain yield traits and the transformation of soluble sugar, starch, soluble protein and fatty acid contents, as well as the related enzyme activity and differential expression of key genes in oil metabolism.
36384833	0	172	theme	oil	7:9	arg1	formation					19:27	[Grain oil quality formation	0:27	[Grain oil quality formation	0:27	[Grain oil quality formation and metabolism-related genes difference expression of Paeonia suffruticosa cv.
36823009	9	0	from	groups	1527:1532	arg1	similar					1507:1513	similar	1507:1513	similar	1507:1513	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	4	1	theme	udder	717:721	arg1	quarter					723:729	one udder quarter	713:729	one udder quarter	713:729	In wk 3, one udder quarter was challenged with lipopolysaccharide (LPS) from Escherichia coli.
36823009	3	2	theme	=	622:622	arg1	n					620:620	n = 10	620:625	n = 10	620:625	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	2	3	theme	high	326:329	arg1	contents					331:338	high contents	326:338	high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence	326:505	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	3	4	dep	nitrogenic	608:617	arg1	n					620:620	n = 10	620:625	n = 10	620:625	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	11	5	theme	increased	1768:1776	arg1	glucose					1785:1791	increased plasma glucose	1768:1791	increased plasma glucose	1768:1791	During mammary inflammation an insulin resistance, shown by increased plasma glucose, insulin, and glucagon, developed similarly in all diet groups.
36823009	6	6	theme	LPS	929:931	arg1	challenge					933:941	LPS challenge	929:941	LPS challenge	929:941	On the day of LPS challenge additional samples were taken hourly for quarter milk and every 3 h for blood.
36823009	15	7	theme	components	2424:2433	arg1	supply					2377:2382	the elevated supply	2364:2382	the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study	2364:2472	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	15	8	theme	intramammary	2274:2285	arg1	LPS					2287:2289	intramammary LPS	2274:2289	intramammary LPS of dairy cows	2274:2303	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	4	9	from	coli	793:796	arg1	lipopolysaccharide					751:768	lipopolysaccharide	751:768	lipopolysaccharide (LPS) from Escherichia coli	751:796	In wk 3, one udder quarter was challenged with lipopolysaccharide (LPS) from Escherichia coli.
36823009	4	9	from	coli	793:796	arg1	LPS					771:773	LPS	771:773	LPS	771:773	In wk 3, one udder quarter was challenged with lipopolysaccharide (LPS) from Escherichia coli.
36823009	9	10	from	similar	1507:1513	arg1	groups					1527:1532	all diet groups	1518:1532	all diet groups for one day	1518:1544	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	11	theme	somatic	1391:1397	arg1	SCC					1411:1413	SCC	1411:1413	SCC	1411:1413	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	11	theme	somatic	1391:1397	arg1	count					1404:1408	somatic cell count	1391:1408	somatic cell count (SCC) in the treated quarter	1391:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	3	12	from	protein	599:605	arg1	rich					585:588	rich	585:588	rich	585:588	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	13	theme	glucogenic	629:638	arg1	precursors					640:649	glucogenic precursors	629:649	glucogenic precursors (glucogenic, n = 11)	629:670	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	13	theme	glucogenic	629:638	arg1	glucogenic					652:661	glucogenic	652:661	glucogenic	652:661	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	14	14	theme	LPS	2107:2109	arg1	challenge					2111:2119	LPS challenge	2107:2119	LPS challenge	2107:2119	On d 9 after LPS challenge, SCC and milk yield and metabolic factors were recovered in all groups.
36823009	9	15	theme	count	1404:1408	arg1	increase					1379:1386	a substantial increase	1365:1386	a substantial increase of somatic cell count (SCC) in the treated quarter	1365:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	15	theme	count	1404:1408	arg1	decrease					1456:1463	a transient decrease	1444:1463	a transient decrease of total milk yield and white blood cells similar in all diet groups for one day	1444:1544	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	10	16	theme	leukocytes	1582:1591	arg1	phagocytosis					1560:1571	The absolute phagocytosis	1547:1571	The absolute phagocytosis of blood leukocytes	1547:1591	The absolute phagocytosis of blood leukocytes was decreased; however, the phagocytosis per cell was increased in glucogenic-fed cows at 6 h after LPS challenge.
36823009	3	17	theme	=	666:666	arg1	n					664:664	n = 11	664:669	n = 11	664:669	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	15	18	theme	glucogenic	2399:2408	arg1	components					2424:2433	nitrogenic, glucogenic, or lipogenic components	2387:2433	nitrogenic, glucogenic, or lipogenic components due to the provided feed	2387:2458	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	15	19	theme	cows	2300:2303	arg1	LPS					2287:2289	intramammary LPS	2274:2289	intramammary LPS of dairy cows	2274:2303	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	1	20	theme	dairy	167:171	arg1	cows					173:176	dairy cows	167:176	dairy cows	167:176	Energy and nutrient deficiency in dairy cows in early lactation is considered to contribute to their increased susceptibility to mastitis.
36823009	0	21	theme	early	102:106	arg1	lactation					108:116	early lactation	102:116	early lactation of dairy cows	102:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	11	22	theme	mammary	1715:1721	arg1	inflammation					1723:1734	mammary inflammation	1715:1734	mammary inflammation	1715:1734	During mammary inflammation an insulin resistance, shown by increased plasma glucose, insulin, and glucagon, developed similarly in all diet groups.
36823009	8	23	theme	triglycerides	1162:1174	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	6	24	theme	quarter	984:990	arg1	milk					992:995	quarter milk	984:995	quarter milk	984:995	On the day of LPS challenge additional samples were taken hourly for quarter milk and every 3 h for blood.
36823009	3	25	theme	concentrate	559:569	arg1	precursors					640:649	glucogenic precursors	629:649	glucogenic precursors (glucogenic, n = 11)	629:670	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	25	theme	concentrate	559:569	arg1	concentrate					559:569	concentrate	559:569	concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10)	559:626	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	25	theme	concentrate	559:569	arg1	glucogenic					652:661	glucogenic	652:661	glucogenic	652:661	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	25	theme	concentrate	559:569	arg1	amounts					548:554	increasing amounts	537:554	increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10)	537:626	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	25	theme	concentrate	559:569	arg1	lipids					676:681	lipids	676:681	lipids (lipogenic, n = 11)	676:701	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	25	theme	concentrate	559:569	arg1	lipogenic					684:692	lipogenic	684:692	lipogenic	684:692	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	9	26	theme	treated	1423:1429	arg1	quarter					1431:1437	the treated quarter	1419:1437	the treated quarter	1419:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	8	27	theme	β-hydroxybutyrate	1177:1193	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	7	28	theme	nitrogenic	1089:1098	arg1	diet					1100:1103	the nitrogenic diet	1085:1103	the nitrogenic diet	1085:1103	Urea concentrations were higher in plasma and milk of cows fed the nitrogenic diet.
36823009	0	29	theme	cows	127:130	arg1	lactation					108:116	early lactation	102:116	early lactation of dairy cows	102:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	8	30	theme	nonesterified	1196:1208	arg1	acids					1216:1220	nonesterified fatty acids	1196:1220	nonesterified fatty acids	1196:1220	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	15	31	theme	provided	2446:2453	arg1	feed					2455:2458	the provided feed	2442:2458	the provided feed	2442:2458	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	8	32	theme	acids	1216:1220	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	6	33	theme	additional	943:952	arg1	samples					954:960	additional samples	943:960	additional samples	943:960	On the day of LPS challenge additional samples were taken hourly for quarter milk and every 3 h for blood.
36823009	0	34	theme	mammary	55:61	arg1	response					70:77	mammary immune response	55:77	mammary immune response to an LPS challenge in early lactation of dairy cows	55:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	2	35	theme	early	401:405	arg1	lactation					407:415	early lactation	401:415	early lactation	401:415	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	9	36	from	quarter	1431:1437	arg1	increase					1379:1386	a substantial increase	1365:1386	a substantial increase of somatic cell count (SCC) in the treated quarter	1365:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	36	from	quarter	1431:1437	arg1	decrease					1456:1463	a transient decrease	1444:1463	a transient decrease of total milk yield and white blood cells similar in all diet groups for one day	1444:1544	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	13	37	dep	h	2075:2075	arg1	challenge					2083:2091	challenge	2083:2091	challenge	2083:2091	Cholesterol did not change, and triglycerides only decreased significantly in lipogenic-fed cows 6 h after challenge.
36823009	12	38	from	challenge	1937:1945	arg1	cows					1965:1968	glucogenic-fed cows	1950:1968	glucogenic-fed cows only	1950:1973	β-hydroxybutyrate and nonesterified fatty acids were decreased at 1 d after LPS challenge in glucogenic-fed cows only.
36823009	9	39	theme	total	1468:1472	arg1	yield					1479:1483	total milk yield	1468:1483	total milk yield	1468:1483	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	0	40	from	Effects	0:6	arg1	response					70:77	mammary immune response	55:77	mammary immune response to an LPS challenge in early lactation of dairy cows	55:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	0	40	from	Effects	0:6	arg1	metabolism					40:49	metabolism	40:49	metabolism	40:49	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	3	41	theme	=	697:697	arg1	n					695:695	n = 11	695:700	n = 11	695:700	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	9	42	theme	yield	1479:1483	arg1	increase					1379:1386	a substantial increase	1365:1386	a substantial increase of somatic cell count (SCC) in the treated quarter	1365:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	42	theme	yield	1479:1483	arg1	decrease					1456:1463	a transient decrease	1444:1463	a transient decrease of total milk yield and white blood cells similar in all diet groups for one day	1444:1544	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	0	43	from	metabolism	40:49	arg1	lactation					108:116	early lactation	102:116	early lactation of dairy cows	102:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	2	44	dep	either	343:348	arg1	nitrogenic					350:359	nitrogenic	350:359	nitrogenic	350:359	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	15	45	theme	dairy	2294:2298	arg1	cows					2300:2303	dairy cows	2294:2303	dairy cows	2294:2303	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	9	46	theme	white	1489:1493	arg1	cells					1501:1505	white blood cells	1489:1505	white blood cells	1489:1505	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	15	47	theme	nitrogenic	2387:2396	arg1	components					2424:2433	nitrogenic, glucogenic, or lipogenic components	2387:2433	nitrogenic, glucogenic, or lipogenic components due to the provided feed	2387:2458	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	9	48	theme	cells	1501:1505	arg1	increase					1379:1386	a substantial increase	1365:1386	a substantial increase of somatic cell count (SCC) in the treated quarter	1365:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	48	theme	cells	1501:1505	arg1	decrease					1456:1463	a transient decrease	1444:1463	a transient decrease of total milk yield and white blood cells similar in all diet groups for one day	1444:1544	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	8	49	theme	insulin-like	1257:1268	arg1	factor-1					1277:1284	insulin-like growth factor-1	1257:1284	insulin-like growth factor-1	1257:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	12	50	theme	fatty	1893:1897	arg1	acids					1899:1903	nonesterified fatty acids	1879:1903	nonesterified fatty acids	1879:1903	β-hydroxybutyrate and nonesterified fatty acids were decreased at 1 d after LPS challenge in glucogenic-fed cows only.
36823009	0	51	theme	different	11:19	arg1	supply					30:35	different nutrient supply	11:35	different nutrient supply	11:35	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	2	52	theme	metabolic	448:456	arg1	status					458:463	the endocrine and metabolic status	430:463	the endocrine and metabolic status	430:463	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	8	53	theme	factor-1	1277:1284	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	8	54	theme	plasma	1115:1120	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	0	55	theme	supply	30:35	arg1	Effects					0:6	Effects	0:6	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.	0:131	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	5	56	theme	LPS	900:902	arg1	challenge					904:912	LPS challenge	900:912	LPS challenge	900:912	Blood and milk were sampled on the day before LPS challenge (d -1), and on d 0, 1, 2, 3, and 9 after LPS challenge.
36823009	13	57	theme	lipogenic-fed	2054:2066	arg1	cows					2068:2071	lipogenic-fed cows	2054:2071	lipogenic-fed cows	2054:2071	Cholesterol did not change, and triglycerides only decreased significantly in lipogenic-fed cows 6 h after challenge.
36823009	2	58	theme	endocrine	434:442	arg1	status					458:463	the endocrine and metabolic status	430:463	the endocrine and metabolic status	430:463	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	2	59	dep	status	458:463	arg1	both					425:428	both	425:428	both	425:428	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	9	60	from	decrease	1456:1463	arg1	quarter					1431:1437	the treated quarter	1419:1437	the treated quarter	1419:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	6	61	theme	challenge	933:941	arg1	day					922:924	the day	918:924	the day of LPS challenge	918:941	On the day of LPS challenge additional samples were taken hourly for quarter milk and every 3 h for blood.
36823009	9	62	theme	immune	1340:1345	arg1	challenge					1347:1355	The mammary immune challenge	1328:1355	The mammary immune challenge	1328:1355	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	8	63	theme	different	1311:1319	arg1	diets					1321:1325	the different diets	1307:1325	the different diets	1307:1325	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	9	64	theme	diet	1522:1525	arg1	groups					1527:1532	all diet groups	1518:1532	all diet groups for one day	1518:1544	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	5	65	theme	LPS	845:847	arg1	d					860:860	d -1	860:863	d -1	860:863	Blood and milk were sampled on the day before LPS challenge (d -1), and on d 0, 1, 2, 3, and 9 after LPS challenge.
36823009	5	65	theme	LPS	845:847	arg1	challenge					849:857	LPS challenge	845:857	LPS challenge (d -1)	845:864	Blood and milk were sampled on the day before LPS challenge (d -1), and on d 0, 1, 2, 3, and 9 after LPS challenge.
36823009	15	66	theme	due	2435:2437	arg1	components					2424:2433	nitrogenic, glucogenic, or lipogenic components	2387:2433	nitrogenic, glucogenic, or lipogenic components due to the provided feed	2387:2458	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	14	67	theme	milk	2130:2133	arg1	yield					2135:2139	milk yield	2130:2139	milk yield	2130:2139	On d 9 after LPS challenge, SCC and milk yield and metabolic factors were recovered in all groups.
36823009	9	68	theme	substantial	1367:1377	arg1	increase					1379:1386	a substantial increase	1365:1386	a substantial increase of somatic cell count (SCC) in the treated quarter	1365:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	3	69	from	rich	585:588	arg1	nitrogenic					608:617	nitrogenic	608:617	nitrogenic	608:617	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	69	from	rich	585:588	arg1	protein					599:605	crude protein	593:605	crude protein (nitrogenic, n = 10)	593:626	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	10	70	theme	blood	1576:1580	arg1	leukocytes					1582:1591	blood leukocytes	1576:1591	blood leukocytes	1576:1591	The absolute phagocytosis of blood leukocytes was decreased; however, the phagocytosis per cell was increased in glucogenic-fed cows at 6 h after LPS challenge.
36823009	15	71	theme	lipogenic	2414:2422	arg1	components					2424:2433	nitrogenic, glucogenic, or lipogenic components	2387:2433	nitrogenic, glucogenic, or lipogenic components due to the provided feed	2387:2458	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	1	72	theme	increased	234:242	arg1	susceptibility					244:257	their increased susceptibility	228:257	their increased susceptibility to mastitis	228:269	Energy and nutrient deficiency in dairy cows in early lactation is considered to contribute to their increased susceptibility to mastitis.
36823009	11	73	theme	plasma	1778:1783	arg1	glucose					1785:1791	increased plasma glucose	1768:1791	increased plasma glucose	1768:1791	During mammary inflammation an insulin resistance, shown by increased plasma glucose, insulin, and glucagon, developed similarly in all diet groups.
36823009	1	74	theme	nutrient	144:151	arg1	deficiency					153:162	Energy and nutrient deficiency	133:162	Energy and nutrient deficiency in dairy cows in early lactation	133:195	Energy and nutrient deficiency in dairy cows in early lactation is considered to contribute to their increased susceptibility to mastitis.
36823009	9	75	theme	cell	1399:1402	arg1	SCC					1411:1413	SCC	1411:1413	SCC	1411:1413	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	75	theme	cell	1399:1402	arg1	count					1404:1408	somatic cell count	1391:1408	somatic cell count (SCC) in the treated quarter	1391:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	9	76	from	count	1404:1408	arg1	quarter					1431:1437	the treated quarter	1419:1437	the treated quarter	1419:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	15	77	theme	immune	2255:2260	arg1	response					2262:2269	the immune response	2251:2269	the immune response to intramammary LPS of dairy cows during early lactation	2251:2326	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	11	78	theme	insulin	1739:1745	arg1	resistance					1747:1756	an insulin resistance	1736:1756	an insulin resistance	1736:1756	During mammary inflammation an insulin resistance, shown by increased plasma glucose, insulin, and glucagon, developed similarly in all diet groups.
36823009	0	79	theme	dairy	121:125	arg1	cows					127:130	dairy cows	121:130	dairy cows	121:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	3	80	dep	lipogenic	684:692	arg1	n					695:695	n = 11	695:700	n = 11	695:700	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	1	81	theme	early	181:185	arg1	lactation					187:195	early lactation	181:195	early lactation	181:195	Energy and nutrient deficiency in dairy cows in early lactation is considered to contribute to their increased susceptibility to mastitis.
36823009	9	82	from	increase	1379:1386	arg1	quarter					1431:1437	the treated quarter	1419:1437	the treated quarter	1419:1437	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	2	83	theme	immune	489:494	arg1	competence					496:505	the mammary immune competence	477:505	the mammary immune competence	477:505	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	3	84	theme	increasing	537:546	arg1	concentrate					559:569	concentrate	559:569	concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10)	559:626	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	84	theme	increasing	537:546	arg1	amounts					548:554	increasing amounts	537:554	increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10)	537:626	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	12	85	theme	glucogenic-fed	1950:1963	arg1	cows					1965:1968	glucogenic-fed cows	1950:1968	glucogenic-fed cows only	1950:1973	β-hydroxybutyrate and nonesterified fatty acids were decreased at 1 d after LPS challenge in glucogenic-fed cows only.
36823009	8	86	theme	fatty	1210:1214	arg1	acids					1216:1220	nonesterified fatty acids	1196:1220	nonesterified fatty acids	1196:1220	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	0	87	theme	immune	63:68	arg1	response					70:77	mammary immune response	55:77	mammary immune response to an LPS challenge in early lactation of dairy cows	55:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	9	88	theme	transient	1446:1454	arg1	decrease					1456:1463	a transient decrease	1444:1463	a transient decrease of total milk yield and white blood cells similar in all diet groups for one day	1444:1544	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	7	89	theme	cows	1076:1079	arg1	plasma					1057:1062	plasma	1057:1062	plasma	1057:1062	Urea concentrations were higher in plasma and milk of cows fed the nitrogenic diet.
36823009	7	89	theme	cows	1076:1079	arg1	milk					1068:1071	milk	1068:1071	milk	1068:1071	Urea concentrations were higher in plasma and milk of cows fed the nitrogenic diet.
36823009	9	90	theme	milk	1474:1477	arg1	yield					1479:1483	total milk yield	1468:1483	total milk yield	1468:1483	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	15	91	theme	early	2312:2316	arg1	lactation					2318:2326	early lactation	2312:2326	early lactation	2312:2326	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	1	92	from	deficiency	153:162	arg1	cows					173:176	dairy cows	167:176	dairy cows	167:176	Energy and nutrient deficiency in dairy cows in early lactation is considered to contribute to their increased susceptibility to mastitis.
36823009	1	92	from	deficiency	153:162	arg1	lactation					187:195	early lactation	181:195	early lactation	181:195	Energy and nutrient deficiency in dairy cows in early lactation is considered to contribute to their increased susceptibility to mastitis.
36823009	1	93	theme	Energy	133:138	arg1	deficiency					153:162	Energy and nutrient deficiency	133:162	Energy and nutrient deficiency in dairy cows in early lactation	133:195	Energy and nutrient deficiency in dairy cows in early lactation is considered to contribute to their increased susceptibility to mastitis.
36823009	8	94	theme	insulin	1234:1240	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	0	95	theme	LPS	85:87	arg1	challenge					89:97	an LPS challenge	82:97	an LPS challenge	82:97	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	2	96	theme	mammary	481:487	arg1	competence					496:505	the mammary immune competence	477:505	the mammary immune competence	477:505	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	8	97	theme	glucagon	1243:1250	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	10	98	theme	LPS	1693:1695	arg1	challenge					1697:1705	LPS challenge	1693:1705	LPS challenge	1693:1705	The absolute phagocytosis of blood leukocytes was decreased; however, the phagocytosis per cell was increased in glucogenic-fed cows at 6 h after LPS challenge.
36823009	12	99	theme	LPS	1933:1935	arg1	challenge					1937:1945	LPS challenge	1933:1945	LPS challenge in glucogenic-fed cows only	1933:1973	β-hydroxybutyrate and nonesterified fatty acids were decreased at 1 d after LPS challenge in glucogenic-fed cows only.
36823009	15	100	from	supply	2377:2382	arg1	study					2468:2472	this study	2463:2472	this study	2463:2472	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	9	101	theme	blood	1495:1499	arg1	cells					1501:1505	white blood cells	1489:1505	white blood cells	1489:1505	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	8	102	theme	growth	1270:1275	arg1	factor-1					1277:1284	insulin-like growth factor-1	1257:1284	insulin-like growth factor-1	1257:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	0	103	from	response	70:77	arg1	lactation					108:116	early lactation	102:116	early lactation of dairy cows	102:130	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	0	104	theme	nutrient	21:28	arg1	supply					30:35	different nutrient supply	11:35	different nutrient supply	11:35	Effects of different nutrient supply on metabolism and mammary immune response to an LPS challenge in early lactation of dairy cows.
36823009	9	105	theme	similar	1507:1513	arg1	yield					1479:1483	total milk yield	1468:1483	total milk yield	1468:1483	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	3	106	theme	crude	593:597	arg1	nitrogenic					608:617	nitrogenic	608:617	nitrogenic	608:617	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	3	106	theme	crude	593:597	arg1	protein					599:605	crude protein	593:605	crude protein (nitrogenic, n = 10)	593:626	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	12	107	theme	nonesterified	1879:1891	arg1	acids					1899:1903	nonesterified fatty acids	1879:1903	nonesterified fatty acids	1879:1903	β-hydroxybutyrate and nonesterified fatty acids were decreased at 1 d after LPS challenge in glucogenic-fed cows only.
36823009	2	108	theme	components	387:396	arg1	contents					331:338	high contents	326:338	high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence	326:505	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	3	109	dep	10	577:578	arg1	to					574:575	to	574:575	to	574:575	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	7	110	theme	Urea	1022:1025	arg1	concentrations					1027:1040	Urea concentrations	1022:1040	Urea concentrations	1022:1040	Urea concentrations were higher in plasma and milk of cows fed the nitrogenic diet.
36823009	3	111	theme	rich	585:588	arg1	concentrate					559:569	concentrate	559:569	concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10)	559:626	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	15	112	theme	elevated	2368:2375	arg1	supply					2377:2382	the elevated supply	2364:2382	the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study	2364:2472	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	2	113	with	diets	315:319	arg1	contents					331:338	high contents	326:338	high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence	326:505	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	11	114	theme	diet	1844:1847	arg1	groups					1849:1854	all diet groups	1840:1854	all diet groups	1840:1854	During mammary inflammation an insulin resistance, shown by increased plasma glucose, insulin, and glucagon, developed similarly in all diet groups.
36823009	2	115	dep	components	387:396	arg1	glucogenic					362:371	glucogenic	362:371	glucogenic	362:371	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	2	115	dep	components	387:396	arg1	lipogenic					377:385	lipogenic	377:385	lipogenic	377:385	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	2	115	dep	components	387:396	arg1	affects					417:423	affects	417:423	affects both the endocrine and metabolic status, as well as the mammary immune competence	417:505	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	2	115	dep	components	387:396	arg1	either					343:348	either	343:348	either	343:348	We have tested the hypothesis that feeding diets with high contents of either nitrogenic, glucogenic, or lipogenic components in early lactation affects both the endocrine and metabolic status, as well as the mammary immune competence.
36823009	15	116	theme	metabolic	2226:2234	arg1	situation					2236:2244	the endocrine and metabolic situation	2208:2244	the endocrine and metabolic situation	2208:2244	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	3	117	dep	glucogenic	652:661	arg1	n					664:664	n = 11	664:669	n = 11	664:669	After calving, cows were fed increasing amounts of concentrate up to 10 kg/d rich in crude protein (nitrogenic, n = 10), glucogenic precursors (glucogenic, n = 11), or lipids (lipogenic, n = 11).
36823009	9	118	theme	mammary	1332:1338	arg1	challenge					1347:1355	The mammary immune challenge	1328:1355	The mammary immune challenge	1328:1355	The mammary immune challenge induced a substantial increase of somatic cell count (SCC) in the treated quarter, and a transient decrease of total milk yield and white blood cells similar in all diet groups for one day.
36823009	10	119	theme	absolute	1551:1558	arg1	phagocytosis					1560:1571	The absolute phagocytosis	1547:1571	The absolute phagocytosis of blood leukocytes	1547:1591	The absolute phagocytosis of blood leukocytes was decreased; however, the phagocytosis per cell was increased in glucogenic-fed cows at 6 h after LPS challenge.
36823009	8	120	theme	glucose	1140:1146	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	15	121	theme	endocrine	2212:2220	arg1	situation					2236:2244	the endocrine and metabolic situation	2208:2244	the endocrine and metabolic situation	2208:2244	In conclusion, the endocrine and metabolic situation, and the immune response to intramammary LPS of dairy cows during early lactation was not substantially influenced by the elevated supply of nitrogenic, glucogenic, or lipogenic components due to the provided feed in this study.
36823009	14	122	theme	metabolic	2145:2153	arg1	factors					2155:2161	metabolic factors	2145:2161	metabolic factors	2145:2161	On d 9 after LPS challenge, SCC and milk yield and metabolic factors were recovered in all groups.
36823009	8	123	theme	cholesterol	1149:1159	arg1	concentrations					1122:1135	plasma concentrations	1115:1135	plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1	1115:1284	However, plasma concentrations of glucose, cholesterol, triglycerides, β-hydroxybutyrate, nonesterified fatty acids, as well as insulin, glucagon, and insulin-like growth factor-1 were not affected by the different diets.
36823009	10	124	theme	glucogenic-fed	1660:1673	arg1	cows					1675:1678	glucogenic-fed cows	1660:1678	glucogenic-fed cows	1660:1678	The absolute phagocytosis of blood leukocytes was decreased; however, the phagocytosis per cell was increased in glucogenic-fed cows at 6 h after LPS challenge.
37110805	5	0	from	composition	811:821	arg1	unique					797:802	unique	797:802	unique	797:802	The reported BC-GTE was found to contain approximately 133 phytonutrients, making it unique in its composition.
37110805	0	1	theme	TNF-α	182:186	arg1	Level					188:192	Serum TNF-α Level	176:192	Serum TNF-α Level: Pilot Study	176:205	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	8	2	from	located	1262:1268	arg1	region					1293:1298	the hippocampal CA1 region	1273:1298	the hippocampal CA1 region	1273:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	10	3	theme	specific	1512:1519	arg1	content					1531:1537	The analyzed BC-GTE's specific flavonoid content	1490:1537	The analyzed BC-GTE's specific flavonoid content	1490:1537	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	11	4	contain	has	1733:1735	arg2	potential					1741:1749	the potential to be used as a GTE-based complementary therapeutic approach	1737:1810	the potential to be used as a GTE-based complementary therapeutic approach	1737:1810	This indicates that the studied BC-GTE has the potential to be used as a GTE-based complementary therapeutic approach.
37110805	11	4	contain	has	1733:1735	arg1	BC-GTE					1726:1731	the studied BC-GTE	1714:1731	the studied BC-GTE	1714:1731	This indicates that the studied BC-GTE has the potential to be used as a GTE-based complementary therapeutic approach.
37110805	9	5	theme	TNF-α	1419:1423	arg1	levels					1394:1399	no elevated levels	1382:1399	no elevated levels of serum-specific TNF-α	1382:1423	Moreover, no elevated levels of serum-specific TNF-α were observed under the LPS-induced neuroinflammatory condition.
37110805	0	6	theme	Gemmotherapy	58:69	arg1	Extract					71:77	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract	0:77	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract	0:77	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	2	7	with	phytochemicals	393:406	arg1	benefits					425:432	therapeutic benefits	413:432	therapeutic benefits	413:432	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	0	8	theme	Serum	176:180	arg1	Level					188:192	Serum TNF-α Level	176:192	Serum TNF-α Level: Pilot Study	176:205	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	7	9	theme	Drosophila	968:977	arg1	tests					998:1002	Drosophila melanogaster-based tests	968:1002	Drosophila melanogaster-based tests	968:1002	Drosophila melanogaster-based tests revealed no cytotoxic but nutritive effects.
37110805	8	10	dep	rats	1093:1096	arg1	pretreated					1099:1108	pretreated	1099:1108	pretreated with the analyzed BC-GTE	1099:1133	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	10	dep	rats	1093:1096	arg1	assessed					1139:1146	assessed	1139:1146	assessed after lipopolysaccharide (LPS) injection	1139:1187	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	11	from	evident	1363:1369	arg1	experiments					1318:1328	control experiments	1310:1328	control experiments	1310:1328	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	9	12	theme	serum-specific	1404:1417	arg1	TNF-α					1419:1423	serum-specific TNF-α	1404:1423	serum-specific TNF-α	1404:1423	Moreover, no elevated levels of serum-specific TNF-α were observed under the LPS-induced neuroinflammatory condition.
37110805	0	13	theme	Neuroprotective	87:101	arg1	Effect					103:108	Neuroprotective Effect	87:108	Neuroprotective Effect	87:108	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	8	14	from	experiments	1318:1328	arg1	evident					1363:1369	evident	1363:1369	evident	1363:1369	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	14	from	experiments	1318:1328	arg1	activation					1335:1344	the activation	1331:1344	the activation of microglia	1331:1357	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	7	15	theme	melanogaster-based	979:996	arg1	tests					998:1002	Drosophila melanogaster-based tests	968:1002	Drosophila melanogaster-based tests	968:1002	Drosophila melanogaster-based tests revealed no cytotoxic but nutritive effects.
37110805	1	16	theme	anti-neuroinflammatory	258:279	arg1	effects					308:314	their anti-neuroinflammatory and anti-neurodegenerative effects	252:314	their anti-neuroinflammatory and anti-neurodegenerative effects	252:314	Many plant-derived flavonoids are known for their anti-neuroinflammatory and anti-neurodegenerative effects.
37110805	8	17	from	size	1233:1236	arg1	cells					1256:1260	the microglial cells	1241:1260	the microglial cells located in the hippocampal CA1 region	1241:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	18	from	region	1293:1298	arg1	located					1262:1268	located	1262:1268	located	1262:1268	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	3	19	theme	fresh	544:548	arg1	buds					550:553	fresh buds	544:553	fresh buds	544:553	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	6	20	theme	flavonoids	902:911	arg1	presence					878:885	the presence	874:885	the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol	874:965	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	8	21	theme	male	1081:1084	arg1	rats					1093:1096	adult male Wistar rats	1075:1096	adult male Wistar rats	1075:1096	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	22	theme	CA1	1289:1291	arg1	region					1293:1298	the hippocampal CA1 region	1273:1298	the hippocampal CA1 region	1273:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	23	theme	microglia	1349:1357	arg1	evident					1363:1369	evident	1363:1369	evident	1363:1369	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	23	theme	microglia	1349:1357	arg1	activation					1335:1344	the activation	1331:1344	the activation of microglia	1331:1357	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	11	24	theme	studied	1718:1724	arg1	BC-GTE					1726:1731	the studied BC-GTE	1714:1731	the studied BC-GTE	1714:1731	This indicates that the studied BC-GTE has the potential to be used as a GTE-based complementary therapeutic approach.
37110805	4	25	theme	associated	650:659	arg1	properties					700:709	the associated antioxidant and anti-neuroinflammatory properties	646:709	the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties	582:709	It provides details about the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties.
37110805	5	26	contain	contain	745:751	arg1	BC-GTE					725:730	The reported BC-GTE	712:730	The reported BC-GTE	712:730	The reported BC-GTE was found to contain approximately 133 phytonutrients, making it unique in its composition.
37110805	5	26	contain	contain	745:751	arg2	phytonutrients					771:784	approximately 133 phytonutrients	753:784	approximately 133 phytonutrients	753:784	The reported BC-GTE was found to contain approximately 133 phytonutrients, making it unique in its composition.
37110805	0	27	theme	Pilot	195:199	arg1	Study					201:205	Pilot Study	195:205	Serum TNF-α Level: Pilot Study	176:205	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	10	28	theme	analyzed	1494:1501	arg1	content					1531:1537	The analyzed BC-GTE's specific flavonoid content	1490:1537	The analyzed BC-GTE's specific flavonoid content	1490:1537	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	2	29	dep	Ribes	365:369	arg1	nigrum					371:376	Ribes nigrum	365:376	Ribes nigrum	365:376	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	8	30	from	increase	1216:1223	arg1	size					1233:1236	body size	1228:1236	body size in the microglial cells located in the hippocampal CA1 region	1228:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	31	theme	lipopolysaccharide	1154:1171	arg1	injection					1179:1187	lipopolysaccharide (LPS) injection	1154:1187	lipopolysaccharide (LPS) injection	1154:1187	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	32	theme	microglial	1245:1254	arg1	cells					1256:1260	the microglial cells	1241:1260	the microglial cells located in the hippocampal CA1 region	1241:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	8	33	theme	analyzed	1119:1126	arg1	BC-GTE					1128:1133	the analyzed BC-GTE	1115:1133	the analyzed BC-GTE	1115:1133	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	5	34	from	unique	797:802	arg1	composition					811:821	its composition	807:821	its composition	807:821	The reported BC-GTE was found to contain approximately 133 phytonutrients, making it unique in its composition.
37110805	1	35	theme	anti-neurodegenerative	285:306	arg1	effects					308:314	their anti-neuroinflammatory and anti-neurodegenerative effects	252:314	their anti-neuroinflammatory and anti-neurodegenerative effects	252:314	Many plant-derived flavonoids are known for their anti-neuroinflammatory and anti-neurodegenerative effects.
37110805	8	36	theme	Wistar	1086:1091	arg1	rats					1093:1096	adult male Wistar rats	1075:1096	adult male Wistar rats	1075:1096	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	1	37	link	plant-derived	213:225	arg1	flavonoids					227:236	Many plant-derived flavonoids	208:236	Many plant-derived flavonoids	208:236	Many plant-derived flavonoids are known for their anti-neuroinflammatory and anti-neurodegenerative effects.
37110805	11	38	theme	GTE-based	1767:1775	arg1	approach					1803:1810	a GTE-based complementary therapeutic approach	1765:1810	a GTE-based complementary therapeutic approach	1765:1810	This indicates that the studied BC-GTE has the potential to be used as a GTE-based complementary therapeutic approach.
37110805	8	39	theme	located	1262:1268	arg1	cells					1256:1260	the microglial cells	1241:1260	the microglial cells located in the hippocampal CA1 region	1241:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	3	40	theme	current	439:445	arg1	study					447:451	The current study	435:451	The current study	435:451	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	8	41	theme	apparent	1207:1214	arg1	increase					1216:1223	any apparent increase	1203:1223	any apparent increase in body size in the microglial cells located in the hippocampal CA1 region	1203:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	10	42	contain	possesses	1633:1641	arg1	it					1630:1631	it	1630:1631	it	1630:1631	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	10	42	contain	possesses	1633:1641	arg2	properties					1682:1691	anti-neuroinflammatory/neuroprotective properties	1643:1691	anti-neuroinflammatory/neuroprotective properties	1643:1691	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	0	43	theme	Microglial	124:133	arg1	Swelling					140:147	Microglial Body Swelling	124:147	Microglial Body Swelling in Hippocampus	124:162	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	0	44	dep	Ethanolic	48:56	arg1	Ribes					34:38	Ribes	34:38	Ribes	34:38	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	9	45	theme	neuroinflammatory	1461:1477	arg1	condition					1479:1487	the LPS-induced neuroinflammatory condition	1445:1487	the LPS-induced neuroinflammatory condition	1445:1487	Moreover, no elevated levels of serum-specific TNF-α were observed under the LPS-induced neuroinflammatory condition.
37110805	10	46	theme	inflammatory	1597:1608	arg1	model					1610:1614	an LPS-induced inflammatory model	1582:1614	an LPS-induced inflammatory model	1582:1614	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	4	47	theme	specific	611:618	arg1	profile					603:609	the phytoconstituent profile	582:609	the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties	582:709	It provides details about the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties.
37110805	2	48	dep	currant	352:358	arg1	Ribes					365:369	Ribes	365:369	Ribes	365:369	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	2	48	dep	currant	352:358	arg1	BC					361:362	BC	361:362	BC	361:362	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	0	49	dep	Level	188:192	arg1	Study					201:205	Pilot Study	195:205	Serum TNF-α Level: Pilot Study	176:205	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	10	50	theme	anti-neuroinflammatory/neuroprotective	1643:1680	arg1	properties					1682:1691	anti-neuroinflammatory/neuroprotective properties	1643:1691	anti-neuroinflammatory/neuroprotective properties	1643:1691	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	2	51	dep	fruits	321:326	arg1	The					317:319	The	317:319	The	317:319	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	2	52	contain	contain	379:385	arg1	leaves					332:337	leaves	332:337	leaves	332:337	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	2	52	contain	contain	379:385	arg2	phytochemicals					393:406	these phytochemicals	387:406	these phytochemicals with therapeutic benefits	387:432	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	2	52	contain	contain	379:385	arg1	fruits					321:326	fruits	321:326	fruits	321:326	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	4	53	theme	antioxidant	661:671	arg1	properties					700:709	the associated antioxidant and anti-neuroinflammatory properties	646:709	the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties	582:709	It provides details about the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties.
37110805	2	54	theme	therapeutic	413:423	arg1	benefits					425:432	therapeutic benefits	413:432	therapeutic benefits	413:432	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	0	55	theme	Body	135:138	arg1	Swelling					140:147	Microglial Body Swelling	124:147	Microglial Body Swelling in Hippocampus	124:162	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	8	56	theme	hippocampal	1277:1287	arg1	region					1293:1298	the hippocampal CA1 region	1273:1298	the hippocampal CA1 region	1273:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	0	57	dep	Ribes	34:38	arg1	nigrum					40:45	Ribes nigrum	34:45	Ribes nigrum	34:45	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	1	58	theme	Many	208:211	arg1	flavonoids					227:236	Many plant-derived flavonoids	208:236	Many plant-derived flavonoids	208:236	Many plant-derived flavonoids are known for their anti-neuroinflammatory and anti-neurodegenerative effects.
37110805	10	59	theme	LPS-induced	1585:1595	arg1	model					1610:1614	an LPS-induced inflammatory model	1582:1614	an LPS-induced inflammatory model	1582:1614	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	10	60	theme	experimental	1555:1566	arg1	data					1568:1571	the experimental data	1551:1571	the experimental data based on an LPS-induced inflammatory model	1551:1614	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
37110805	3	61	from	report	464:469	arg1	BC-GTE					514:519	BC-GTE	514:519	BC-GTE	514:519	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	3	61	from	report	464:469	arg1	extract					505:511	a standardized BC gemmotherapy extract	474:511	a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds	474:553	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	3	62	theme	gemmotherapy	492:503	arg1	BC-GTE					514:519	BC-GTE	514:519	BC-GTE	514:519	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	3	62	theme	gemmotherapy	492:503	arg1	extract					505:511	a standardized BC gemmotherapy extract	474:511	a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds	474:553	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	8	63	theme	body	1228:1231	arg1	size					1233:1236	body size	1228:1236	body size in the microglial cells located in the hippocampal CA1 region	1228:1298	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	5	64	theme	reported	716:723	arg1	BC-GTE					725:730	The reported BC-GTE	712:730	The reported BC-GTE	712:730	The reported BC-GTE was found to contain approximately 133 phytonutrients, making it unique in its composition.
37110805	8	65	theme	adult	1075:1079	arg1	rats					1093:1096	adult male Wistar rats	1075:1096	adult male Wistar rats	1075:1096	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	2	66	theme	currant	352:358	arg1	fruits					321:326	fruits	321:326	fruits	321:326	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	6	67	theme	first	849:853	arg1	report					855:860	the first report	845:860	the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol	845:965	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	6	67	theme	first	849:853	arg1	this					837:840	this	837:840	this	837:840	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	4	68	theme	anti-neuroinflammatory	677:698	arg1	properties					700:709	the associated antioxidant and anti-neuroinflammatory properties	646:709	the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties	582:709	It provides details about the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties.
37110805	2	69	theme	black	346:350	arg1	currant					352:358	the black currant	342:358	the black currant (BC, Ribes nigrum)	342:377	The fruits and leaves of the black currant (BC, Ribes nigrum) contain these phytochemicals with therapeutic benefits.
37110805	9	70	theme	LPS-induced	1449:1459	arg1	condition					1479:1487	the LPS-induced neuroinflammatory condition	1445:1487	the LPS-induced neuroinflammatory condition	1445:1487	Moreover, no elevated levels of serum-specific TNF-α were observed under the LPS-induced neuroinflammatory condition.
37110805	0	71	from	Swelling	140:147	arg1	Hippocampus					152:162	Hippocampus	152:162	Hippocampus	152:162	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	7	72	theme	nutritive	1030:1038	arg1	effects					1040:1046	no cytotoxic but nutritive effects	1013:1046	no cytotoxic but nutritive effects	1013:1046	Drosophila melanogaster-based tests revealed no cytotoxic but nutritive effects.
37110805	3	73	theme	BC	489:490	arg1	BC-GTE					514:519	BC-GTE	514:519	BC-GTE	514:519	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	3	73	theme	BC	489:490	arg1	extract					505:511	a standardized BC gemmotherapy extract	474:511	a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds	474:553	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	3	74	theme	standardized	476:487	arg1	BC-GTE					514:519	BC-GTE	514:519	BC-GTE	514:519	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	3	74	theme	standardized	476:487	arg1	extract					505:511	a standardized BC gemmotherapy extract	474:511	a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds	474:553	The current study presents a report on a standardized BC gemmotherapy extract (BC-GTE) that is prepared from fresh buds.
37110805	0	75	theme	Ethanolic	48:56	arg1	Extract					71:77	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract	0:77	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract	0:77	The Flavonoid Rich Black Currant (Ribes nigrum) Ethanolic Gemmotherapy Extract Elicits Neuroprotective Effect by Preventing Microglial Body Swelling in Hippocampus and Reduces Serum TNF-α Level: Pilot Study.
37110805	11	76	theme	therapeutic	1791:1801	arg1	approach					1803:1810	a GTE-based complementary therapeutic approach	1765:1810	a GTE-based complementary therapeutic approach	1765:1810	This indicates that the studied BC-GTE has the potential to be used as a GTE-based complementary therapeutic approach.
37110805	6	77	theme	significant	890:900	arg1	flavonoids					902:911	significant flavonoids	890:911	significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol	890:965	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	6	77	theme	significant	890:900	arg1	luteolin					921:928	luteolin	921:928	luteolin	921:928	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	6	77	theme	significant	890:900	arg1	apigenin					942:949	apigenin	942:949	apigenin	942:949	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	6	77	theme	significant	890:900	arg1	kaempferol					956:965	kaempferol	956:965	kaempferol	956:965	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	6	77	theme	significant	890:900	arg1	quercetin					931:939	quercetin	931:939	quercetin	931:939	Furthermore, this is the first report to quantify the presence of significant flavonoids such as luteolin, quercetin, apigenin, and kaempferol.
37110805	7	78	theme	cytotoxic	1016:1024	arg1	effects					1040:1046	no cytotoxic but nutritive effects	1013:1046	no cytotoxic but nutritive effects	1013:1046	Drosophila melanogaster-based tests revealed no cytotoxic but nutritive effects.
37110805	8	79	theme	control	1310:1316	arg1	experiments					1318:1328	control experiments	1310:1328	control experiments	1310:1328	We also demonstrated that adult male Wistar rats, pretreated with the analyzed BC-GTE and assessed after lipopolysaccharide (LPS) injection, did not show any apparent increase in body size in the microglial cells located in the hippocampal CA1 region, while in control experiments, the activation of microglia was evident.
37110805	9	80	theme	elevated	1385:1392	arg1	levels					1394:1399	no elevated levels	1382:1399	no elevated levels of serum-specific TNF-α	1382:1423	Moreover, no elevated levels of serum-specific TNF-α were observed under the LPS-induced neuroinflammatory condition.
37110805	4	81	theme	phytoconstituent	586:601	arg1	profile					603:609	the phytoconstituent profile	582:609	the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties	582:709	It provides details about the phytoconstituent profile specific to the extract as well as the associated antioxidant and anti-neuroinflammatory properties.
37110805	11	82	theme	complementary	1777:1789	arg1	approach					1803:1810	a GTE-based complementary therapeutic approach	1765:1810	a GTE-based complementary therapeutic approach	1765:1810	This indicates that the studied BC-GTE has the potential to be used as a GTE-based complementary therapeutic approach.
37110805	1	83	theme	plant-derived	213:225	arg1	flavonoids					227:236	Many plant-derived flavonoids	208:236	Many plant-derived flavonoids	208:236	Many plant-derived flavonoids are known for their anti-neuroinflammatory and anti-neurodegenerative effects.
37110805	10	84	theme	flavonoid	1521:1529	arg1	content					1531:1537	The analyzed BC-GTE's specific flavonoid content	1490:1537	The analyzed BC-GTE's specific flavonoid content	1490:1537	The analyzed BC-GTE's specific flavonoid content, along with the experimental data based on an LPS-induced inflammatory model, suggest that it possesses anti-neuroinflammatory/neuroprotective properties.
36268225	3	0	theme	omics	971:975	arg1	IBDMDB					987:992	IBDMDB	987:992	IBDMDB	987:992	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	3	0	theme	omics	971:975	arg1	Database					977:984	the Inflammatory Bowel Disease Multi'omics Database	934:984	the Inflammatory Bowel Disease Multi'omics Database (IBDMDB)	934:993	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	1	1	from	abundance	429:437	arg1	colitis					545:551	ulcerative colitis	534:551	ulcerative colitis (UC)	534:556	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	1	from	abundance	429:437	arg1	CD					526:527	CD	526:527	CD	526:527	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	1	from	abundance	429:437	arg1	UC					554:555	UC	554:555	UC	554:555	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	1	from	abundance	429:437	arg1	disease					517:523	Crohn's disease	509:523	Crohn's disease (CD)	509:528	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	6	2	theme	UC	1456:1457	arg1	patients					1459:1466	both CD and UC patients	1444:1466	patients	1459:1466	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	11	3	theme	taxonomic	2574:2582	arg1	composition					2599:2609	the taxonomic and functional composition	2570:2609	the taxonomic and functional composition of intestinal bacteria in CD and UC patients	2570:2654	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	9	4	theme	sulfur	2295:2300	arg1	systems					2308:2314	sulfur relay systems	2295:2314	sulfur relay systems encoded by <i>E. coli</i>	2295:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	0	5	theme	datasets	197:204	arg1	analysis					178:185	a comprehensive metagenomic analysis	150:185	a comprehensive metagenomic analysis of IBDMDB datasets	150:204	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	1	6	theme	Enterobacteriaceae	442:459	arg1	species					461:467	Enterobacteriaceae species	442:467	Enterobacteriaceae species	442:467	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	11	7	from	Conclusions	2513:2523	arg1	conclusion					2528:2537	conclusion	2528:2537	conclusion	2528:2537	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	1	8	theme	intestinal	278:287	arg1	Enterobacteriaceae					289:306	the intestinal Enterobacteriaceae	274:306	the intestinal Enterobacteriaceae	274:306	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	11	9	theme	Enterobacteriaceae	2685:2702	arg1	species					2704:2710	Enterobacteriaceae species	2685:2710	Enterobacteriaceae species	2685:2710	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	9	10	theme	systems	2308:2314	arg1	pathways					2197:2204	Biosynthetic pathways	2184:2204	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i>	2184:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	4	11	theme	healthy	1109:1115	arg1	HCs					1127:1129	HCs	1127:1129	HCs	1127:1129	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	4	11	theme	healthy	1109:1115	arg1	controls					1117:1124	125 healthy controls	1105:1124	125 healthy controls (HCs)	1105:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	1	12	theme	metagenomics	638:649	arg1	analysis					651:658	comprehensive comparative metagenomics analysis	612:658	comprehensive comparative metagenomics analysis	612:658	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	2	13	with	patients	776:783	arg1	UC					797:798	UC	797:798	UC	797:798	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	2	13	with	patients	776:783	arg1	CD					790:791	CD	790:791	CD	790:791	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	1	14	theme	related	479:485	arg1	pathways					497:504	their related metabolic pathways	473:504	their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC)	473:556	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	11	15	theme	<i>Klebsiella</i>	2739:2755	arg1	species					2757:2763	<i>Klebsiella</i> species	2739:2763	<i>Klebsiella</i> species	2739:2763	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	6	16	theme	<i>Klebsiella	1533:1545	arg1	quasipneumoniae</i>					1547:1565	<i>Klebsiella quasipneumoniae</i>	1533:1565	<i>Klebsiella quasipneumoniae</i>	1533:1565	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	2	17	theme	patients	776:783	arg1	microbiome					762:771	the gut microbiome	754:771	the gut microbiome of patients with CD and UC	754:798	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	4	18	from	patients	1091:1098	arg1	samples					1054:1060	fecal samples	1048:1060	fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs)	1048:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	10	19	theme	<i>K.	2483:2487	arg1	strains					2504:2510	<i>K. pneumoniae</i> strains	2483:2510	<i>K. pneumoniae</i> strains	2483:2510	Menaquinol biosynthetic pathways were associated with UC that belonged to <i>K. pneumoniae</i> strains.
36268225	8	20	from	abundance	2115:2123	arg1	community					2173:2181	their intestinal bacterial community	2146:2181	their intestinal bacterial community	2146:2181	Our analysis also showed a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community.
36268225	7	21	theme	<i>Citrobacter	1943:1956	arg1	portucalensis</i>					1958:1974	<i>Citrobacter portucalensis</i>	1943:1974	<i>Citrobacter portucalensis</i>	1943:1974	Four species were uniquely differentially abundant and enriched in the UC cohort, including <i>Citrobacter portucalensis</i>, <i>Citrobacter pasteurii</i>, <i>Citrobacter werkmanii</i>, and <i>Proteus hauseri</i>.
36268225	4	22	from	patients	1074:1081	arg1	samples					1054:1060	fecal samples	1048:1060	fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs)	1048:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	1	23	from	colitis	545:551	arg1	abundance					429:437	the abundance	425:437	the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people	425:583	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	11	24	theme	functional	2588:2597	arg1	composition					2599:2609	the taxonomic and functional composition	2570:2609	the taxonomic and functional composition of intestinal bacteria in CD and UC patients	2570:2654	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	9	25	theme	CD	2377:2378	arg1	samples					2380:2386	the CD samples	2373:2386	the CD samples	2373:2386	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	0	26	theme	metagenomic	166:176	arg1	analysis					178:185	a comprehensive metagenomic analysis	150:185	a comprehensive metagenomic analysis of IBDMDB datasets	150:204	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	3	27	theme	Methods	830:836	arg1	datasets					850:857	Methods Metagenomic datasets	830:857	Methods Metagenomic datasets	830:857	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	11	28	theme	CD	2637:2638	arg1	patients					2647:2654	CD and UC patients	2637:2654	CD and UC patients	2637:2654	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	0	29	theme	major	76:80	arg1	Overrepresentation					0:17	Overrepresentation	0:17	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i>	0:67	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	0	29	theme	major	76:80	arg1	signature					97:105	the major gut microbiome signature	72:105	the major gut microbiome signature in Crohn's disease and ulcerative colitis	72:147	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	6	30	theme	<i>Proteus	1598:1607	arg1	mirabilis</i>					1609:1621	<i>Proteus mirabilis</i>	1598:1621	<i>Proteus mirabilis</i>	1598:1621	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	11	31	theme	bacteria	2625:2632	arg1	composition					2599:2609	the taxonomic and functional composition	2570:2609	the taxonomic and functional composition of intestinal bacteria in CD and UC patients	2570:2654	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	0	32	theme	microbiome	86:95	arg1	Overrepresentation					0:17	Overrepresentation	0:17	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i>	0:67	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	0	32	theme	microbiome	86:95	arg1	signature					97:105	the major gut microbiome signature	72:105	the major gut microbiome signature in Crohn's disease and ulcerative colitis	72:147	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	6	33	theme	<i>Morganella	1718:1730	arg1	morganii</i>					1732:1743	<i>Morganella morganii</i>	1718:1743	<i>Morganella morganii</i>	1718:1743	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	11	34	theme	UC	2644:2645	arg1	patients					2647:2654	CD and UC patients	2637:2654	CD and UC patients	2637:2654	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	11	35	theme	healthy	2554:2560	arg1	people					2562:2567	healthy people	2554:2567	healthy people	2554:2567	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	1	36	theme	comprehensive	612:624	arg1	analysis					651:658	comprehensive comparative metagenomics analysis	612:658	comprehensive comparative metagenomics analysis	612:658	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	6	37	theme	gut	1359:1361	arg1	microbiome					1363:1372	the gut microbiome	1355:1372	the gut microbiome of HCs	1355:1379	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	1	38	theme	bowel	380:384	arg1	disease					386:392	inflammatory bowel disease	367:392	inflammatory bowel disease (IBD)	367:398	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	38	theme	bowel	380:384	arg1	IBD					395:397	IBD	395:397	IBD	395:397	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	8	39	theme	increased	2105:2113	arg1	abundance					2115:2123	a dramatically increased abundance	2090:2123	a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community	2090:2181	Our analysis also showed a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community.
36268225	1	40	theme	ulcerative	534:543	arg1	colitis					545:551	ulcerative colitis	534:551	ulcerative colitis (UC)	534:556	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	40	theme	ulcerative	534:543	arg1	UC					554:555	UC	554:555	UC	554:555	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	5	41	theme	bacterial	1175:1183	arg1	structure					1195:1203	bacterial community structure	1175:1203	bacterial community structure	1175:1203	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	3	42	theme	Human	894:898	arg1	Project					911:917	the Integrative Human Microbiome Project	878:917	the Integrative Human Microbiome Project (HMP2)	878:924	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	3	42	theme	Human	894:898	arg1	HMP2					920:923	HMP2	920:923	HMP2	920:923	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	4	43	theme	metagenome-wide	1009:1023	arg1	studies					1037:1043	metagenome-wide association studies	1009:1043	metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs)	1009:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	1	44	theme	crucial	316:322	arg1	role					324:327	a crucial role	314:327	a crucial role	314:327	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	2	45	theme	current	668:674	arg1	study					676:680	the current study	664:680	the current study	664:680	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	8	46	theme	intestinal	2152:2161	arg1	community					2173:2181	their intestinal bacterial community	2146:2181	their intestinal bacterial community	2146:2181	Our analysis also showed a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community.
36268225	4	47	from	studies	1037:1043	arg1	samples					1054:1060	fecal samples	1048:1060	fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs)	1048:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	7	48	from	cohort	1925:1930	arg1	enriched					1906:1913	enriched	1906:1913	enriched	1906:1913	Four species were uniquely differentially abundant and enriched in the UC cohort, including <i>Citrobacter portucalensis</i>, <i>Citrobacter pasteurii</i>, <i>Citrobacter werkmanii</i>, and <i>Proteus hauseri</i>.
36268225	9	49	theme	aerobactin	2209:2218	arg1	siderophore					2220:2230	aerobactin siderophore	2209:2230	aerobactin siderophore	2209:2230	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	0	50	theme	Enterobacteriaceae	22:39	arg1	Overrepresentation					0:17	Overrepresentation	0:17	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i>	0:67	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	0	50	theme	Enterobacteriaceae	22:39	arg1	signature					97:105	the major gut microbiome signature	72:105	the major gut microbiome signature in Crohn's disease and ulcerative colitis	72:147	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	5	51	theme	biosynthetic	1312:1323	arg1	pathways					1325:1332	Enterobacteriaceae-related biosynthetic pathways	1285:1332	Enterobacteriaceae-related biosynthetic pathways	1285:1332	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	11	52	from	composition	2599:2609	arg1	patients					2647:2654	CD and UC patients	2637:2654	CD and UC patients	2637:2654	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	0	53	theme	<i>Escherichia	45:58	arg1	Overrepresentation					0:17	Overrepresentation	0:17	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i>	0:67	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	0	53	theme	<i>Escherichia	45:58	arg1	signature					97:105	the major gut microbiome signature	72:105	the major gut microbiome signature in Crohn's disease and ulcerative colitis	72:147	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	3	54	theme	Disease	957:963	arg1	IBDMDB					987:992	IBDMDB	987:992	IBDMDB	987:992	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	3	54	theme	Disease	957:963	arg1	Database					977:984	the Inflammatory Bowel Disease Multi'omics Database	934:984	the Inflammatory Bowel Disease Multi'omics Database (IBDMDB)	934:993	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	6	55	theme	Enterobacteriaceae	1386:1403	arg1	species					1405:1411	six Enterobacteriaceae species	1382:1411	six Enterobacteriaceae species	1382:1411	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	3	56	theme	Inflammatory	938:949	arg1	Disease					957:963	the Inflammatory Bowel Disease	934:963	the Inflammatory Bowel Disease Multi'omics Database (IBDMDB)	934:993	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	4	57	theme	CD	1071:1072	arg1	patients					1074:1081	191 CD patients	1067:1081	191 CD patients	1067:1081	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	9	58	theme	common	2254:2259	arg1	antigen					2261:2267	enterobacterial common antigen	2238:2267	enterobacterial common antigen	2238:2267	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	5	59	theme	community	1185:1193	arg1	structure					1195:1203	bacterial community structure	1175:1203	bacterial community structure	1175:1203	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	7	60	theme	<i>Citrobacter	1977:1990	arg1	pasteurii</i>					1992:2004	<i>Citrobacter pasteurii</i>	1977:2004	<i>Citrobacter pasteurii</i>	1977:2004	Four species were uniquely differentially abundant and enriched in the UC cohort, including <i>Citrobacter portucalensis</i>, <i>Citrobacter pasteurii</i>, <i>Citrobacter werkmanii</i>, and <i>Proteus hauseri</i>.
36268225	0	61	dep	Enterobacteriaceae	22:39	arg1	coli</i>					60:67	coli</i>	60:67	coli</i>	60:67	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	7	62	theme	UC	1922:1923	arg1	cohort					1925:1930	the UC cohort	1918:1930	the UC cohort	1918:1930	Four species were uniquely differentially abundant and enriched in the UC cohort, including <i>Citrobacter portucalensis</i>, <i>Citrobacter pasteurii</i>, <i>Citrobacter werkmanii</i>, and <i>Proteus hauseri</i>.
36268225	1	63	dep	Objectives	207:216	arg1	suggest					261:267	suggest	261:267	suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD)	261:398	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	64	from	species	461:467	arg1	colitis					545:551	ulcerative colitis	534:551	ulcerative colitis (UC)	534:556	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	64	from	species	461:467	arg1	CD					526:527	CD	526:527	CD	526:527	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	64	from	species	461:467	arg1	UC					554:555	UC	554:555	UC	554:555	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	64	from	species	461:467	arg1	disease					517:523	Crohn's disease	509:523	Crohn's disease (CD)	509:528	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	4	65	theme	fecal	1048:1052	arg1	samples					1054:1060	fecal samples	1048:1060	fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs)	1048:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	9	66	theme	metabolism	2279:2288	arg1	pathways					2197:2204	Biosynthetic pathways	2184:2204	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i>	2184:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	0	67	theme	IBDMDB	190:195	arg1	datasets					197:204	IBDMDB datasets	190:204	IBDMDB datasets	190:204	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	7	68	from	enriched	1906:1913	arg1	cohort					1925:1930	the UC cohort	1918:1930	the UC cohort	1918:1930	Four species were uniquely differentially abundant and enriched in the UC cohort, including <i>Citrobacter portucalensis</i>, <i>Citrobacter pasteurii</i>, <i>Citrobacter werkmanii</i>, and <i>Proteus hauseri</i>.
36268225	2	69	theme	healthy	812:818	arg1	subjects					820:827	healthy subjects	812:827	healthy subjects	812:827	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	10	70	theme	biosynthetic	2420:2431	arg1	pathways					2433:2440	Menaquinol biosynthetic pathways	2409:2440	Menaquinol biosynthetic pathways	2409:2440	Menaquinol biosynthetic pathways were associated with UC that belonged to <i>K. pneumoniae</i> strains.
36268225	2	71	theme	gut	758:760	arg1	microbiome					762:771	the gut microbiome	754:771	the gut microbiome of patients with CD and UC	754:798	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	2	72	theme	population	740:749	arg1	alternations					703:714	the alternations	699:714	the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC	699:798	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	9	73	theme	relay	2302:2306	arg1	systems					2308:2314	sulfur relay systems	2295:2314	sulfur relay systems encoded by <i>E. coli</i>	2295:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	1	74	theme	species	461:467	arg1	abundance					429:437	the abundance	425:437	the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people	425:583	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	6	75	theme	<i>Klebsiella	1568:1580	arg1	pneumoniae</i>					1582:1595	<i>Klebsiella pneumoniae</i>	1568:1595	<i>Klebsiella pneumoniae</i>	1568:1595	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	4	76	from	controls	1117:1124	arg1	samples					1054:1060	fecal samples	1048:1060	fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs)	1048:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	1	77	theme	comparative	626:636	arg1	analysis					651:658	comprehensive comparative metagenomics analysis	612:658	comprehensive comparative metagenomics analysis	612:658	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	78	from	changes	414:420	arg1	abundance					429:437	the abundance	425:437	the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people	425:583	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	4	79	theme	UC	1088:1089	arg1	patients					1091:1098	132 UC patients	1084:1098	132 UC patients	1084:1098	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	9	80	theme	<i>E.	2327:2331	arg1	coli</i>					2333:2340	<i>E. coli</i>	2327:2340	<i>E. coli</i>	2327:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	0	81	theme	ulcerative	130:139	arg1	colitis					141:147	ulcerative colitis	130:147	ulcerative colitis	130:147	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	1	82	theme	metabolic	487:495	arg1	pathways					497:504	their related metabolic pathways	473:504	their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC)	473:556	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	10	83	theme	pneumoniae</i>	2489:2502	arg1	strains					2504:2510	<i>K. pneumoniae</i> strains	2483:2510	<i>K. pneumoniae</i> strains	2483:2510	Menaquinol biosynthetic pathways were associated with UC that belonged to <i>K. pneumoniae</i> strains.
36268225	0	84	from	signature	97:105	arg1	colitis					141:147	ulcerative colitis	130:147	ulcerative colitis	130:147	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	0	84	from	signature	97:105	arg1	disease					118:124	Crohn's disease	110:124	Crohn's disease	110:124	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	2	85	from	alternations	703:714	arg1	microbiome					762:771	the gut microbiome	754:771	the gut microbiome of patients with CD and UC	754:798	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	6	86	theme	<i>Klebsiella	1504:1516	arg1	variicola</i>					1518:1530	<i>Klebsiella variicola</i>	1504:1530	<i>Klebsiella variicola</i>	1504:1530	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	0	87	theme	comprehensive	152:164	arg1	analysis					178:185	a comprehensive metagenomic analysis	150:185	a comprehensive metagenomic analysis of IBDMDB datasets	150:204	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	6	88	theme	<i>Citrobacter	1750:1763	arg1	amalonaticus</i>					1765:1780	<i>Citrobacter amalonaticus</i>	1750:1780	<i>Citrobacter amalonaticus</i>	1750:1780	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	1	89	theme	healthy	570:576	arg1	people					578:583	healthy people	570:583	healthy people	570:583	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	6	90	theme	<i>Citrobacter	1624:1637	arg1	freundii</i>					1639:1650	<i>Citrobacter freundii</i>	1624:1650	<i>Citrobacter freundii</i>	1624:1650	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	2	91	theme	Enterobacterales	723:738	arg1	population					740:749	the Enterobacterales population	719:749	the Enterobacterales population	719:749	In the current study, we investigated the alternations of the Enterobacterales population in the gut microbiome of patients with CD and UC compared to healthy subjects.
36268225	5	92	theme	functional	1260:1269	arg1	analysis					1271:1278	functional analysis	1260:1278	functional analysis	1260:1278	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	0	93	theme	gut	82:84	arg1	Overrepresentation					0:17	Overrepresentation	0:17	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i>	0:67	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	0	93	theme	gut	82:84	arg1	signature					97:105	the major gut microbiome signature	72:105	the major gut microbiome signature in Crohn's disease and ulcerative colitis	72:147	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	5	94	theme	abundant	1241:1248	arg1	bacteria					1250:1257	differentially abundant bacteria	1226:1257	differentially abundant bacteria	1226:1257	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	3	95	theme	Metagenomic	838:848	arg1	datasets					850:857	Methods Metagenomic datasets	830:857	Methods Metagenomic datasets	830:857	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	11	96	theme	intestinal	2614:2623	arg1	bacteria					2625:2632	intestinal bacteria	2614:2632	intestinal bacteria	2614:2632	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	1	97	theme	converging	230:239	arg1	strands					241:247	converging strands	230:247	converging strands of research	230:259	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	8	98	theme	<i>E.	2128:2132	arg1	coli</i>					2134:2141	<i>E. coli</i>	2128:2141	<i>E. coli</i>	2128:2141	Our analysis also showed a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community.
36268225	6	99	theme	HCs	1377:1379	arg1	microbiome					1363:1372	the gut microbiome	1355:1372	the gut microbiome of HCs	1355:1379	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	3	100	theme	Integrative	882:892	arg1	Project					911:917	the Integrative Human Microbiome Project	878:917	the Integrative Human Microbiome Project (HMP2)	878:924	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	3	100	theme	Integrative	882:892	arg1	HMP2					920:923	HMP2	920:923	HMP2	920:923	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	6	101	from	cohort	1843:1848	arg1	enriched					1824:1831	enriched	1824:1831	enriched	1824:1831	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	6	102	theme	CD	1840:1841	arg1	cohort					1843:1848	the CD cohort	1836:1848	the CD cohort	1836:1848	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	9	103	theme	antigen	2261:2267	arg1	pathways					2197:2204	Biosynthetic pathways	2184:2204	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i>	2184:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	8	104	theme	coli</i>	2134:2141	arg1	abundance					2115:2123	a dramatically increased abundance	2090:2123	a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community	2090:2181	Our analysis also showed a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community.
36268225	1	105	theme	inflammatory	367:378	arg1	disease					386:392	inflammatory bowel disease	367:392	inflammatory bowel disease (IBD)	367:398	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	105	theme	inflammatory	367:378	arg1	IBD					395:397	IBD	395:397	IBD	395:397	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	106	theme	disease	386:392	arg1	progression					352:362	progression	352:362	progression	352:362	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	106	theme	disease	386:392	arg1	development					336:346	the development	332:346	the development	332:346	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	6	107	theme	<i>Klebsiella	1691:1703	arg1	oxytoca</i>					1705:1715	<i>Klebsiella oxytoca</i>	1691:1715	<i>Klebsiella oxytoca</i>	1691:1715	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	6	108	from	enriched	1824:1831	arg1	cohort					1843:1848	the CD cohort	1836:1848	the CD cohort	1836:1848	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	9	109	theme	Biosynthetic	2184:2195	arg1	pathways					2197:2204	Biosynthetic pathways	2184:2204	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i>	2184:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	6	110	theme	CD	1449:1450	arg1	patients					1459:1466	both CD and UC patients	1444:1466	patients	1459:1466	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	7	111	theme	<i>Proteus	2041:2050	arg1	hauseri</i>					2052:2062	<i>Proteus hauseri</i>	2041:2062	<i>Proteus hauseri</i>	2041:2062	Four species were uniquely differentially abundant and enriched in the UC cohort, including <i>Citrobacter portucalensis</i>, <i>Citrobacter pasteurii</i>, <i>Citrobacter werkmanii</i>, and <i>Proteus hauseri</i>.
36268225	0	112	dep	signature	97:105	arg1	analysis					178:185	a comprehensive metagenomic analysis	150:185	a comprehensive metagenomic analysis of IBDMDB datasets	150:204	Overrepresentation of Enterobacteriaceae and <i>Escherichia coli</i> is the major gut microbiome signature in Crohn's disease and ulcerative colitis; a comprehensive metagenomic analysis of IBDMDB datasets.
36268225	3	113	theme	Microbiome	900:909	arg1	Project					911:917	the Integrative Human Microbiome Project	878:917	the Integrative Human Microbiome Project (HMP2)	878:924	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	3	113	theme	Microbiome	900:909	arg1	HMP2					920:923	HMP2	920:923	HMP2	920:923	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	1	114	from	disease	517:523	arg1	abundance					429:437	the abundance	425:437	the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people	425:583	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	11	115	theme	<i>E.	2720:2724	arg1	coli</i>					2726:2733	<i>E. coli</i>	2720:2733	<i>E. coli</i>	2720:2733	Conclusions In conclusion, compared with healthy people, the taxonomic and functional composition of intestinal bacteria in CD and UC patients was significantly shifted to Enterobacteriaceae species, mainly <i>E. coli</i> and <i>Klebsiella</i> species.
36268225	6	116	theme	<i>Citrobacter	1657:1670	arg1	youngae</i>					1672:1682	<i>Citrobacter youngae</i>	1657:1682	<i>Citrobacter youngae</i>	1657:1682	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	4	117	theme	association	1025:1035	arg1	studies					1037:1043	metagenome-wide association studies	1009:1043	metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs)	1009:1130	We performed metagenome-wide association studies on fecal samples from 191 CD patients, 132 UC patients, and 125 healthy controls (HCs).
36268225	9	118	theme	siderophore	2220:2230	arg1	pathways					2197:2204	Biosynthetic pathways	2184:2204	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i>	2184:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	5	119	theme	Enterobacteriaceae-related	1285:1310	arg1	pathways					1325:1332	Enterobacteriaceae-related biosynthetic pathways	1285:1332	Enterobacteriaceae-related biosynthetic pathways	1285:1332	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	5	120	theme	metagenomics	1145:1156	arg1	dataset					1158:1164	the metagenomics dataset	1141:1164	the metagenomics dataset	1141:1164	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	7	121	theme	<i>Citrobacter	2007:2020	arg1	werkmanii</i>					2022:2034	<i>Citrobacter werkmanii</i>	2007:2034	<i>Citrobacter werkmanii</i>	2007:2034	Four species were uniquely differentially abundant and enriched in the UC cohort, including <i>Citrobacter portucalensis</i>, <i>Citrobacter pasteurii</i>, <i>Citrobacter werkmanii</i>, and <i>Proteus hauseri</i>.
36268225	1	122	from	pathways	497:504	arg1	colitis					545:551	ulcerative colitis	534:551	ulcerative colitis (UC)	534:556	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	122	from	pathways	497:504	arg1	CD					526:527	CD	526:527	CD	526:527	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	122	from	pathways	497:504	arg1	UC					554:555	UC	554:555	UC	554:555	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	1	122	from	pathways	497:504	arg1	disease					517:523	Crohn's disease	509:523	Crohn's disease (CD)	509:528	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	9	123	theme	LPS	2233:2235	arg1	pathways					2197:2204	Biosynthetic pathways	2184:2204	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i>	2184:2340	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	1	124	theme	pathways	497:504	arg1	abundance					429:437	the abundance	425:437	the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people	425:583	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	3	125	theme	Multi	965:969	arg1	IBDMDB					987:992	IBDMDB	987:992	IBDMDB	987:992	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	3	125	theme	Multi	965:969	arg1	Database					977:984	the Inflammatory Bowel Disease Multi'omics Database	934:984	the Inflammatory Bowel Disease Multi'omics Database (IBDMDB)	934:993	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	9	126	theme	enterobacterial	2238:2252	arg1	antigen					2261:2267	enterobacterial common antigen	2238:2267	enterobacterial common antigen	2238:2267	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	10	127	theme	Menaquinol	2409:2418	arg1	pathways					2433:2440	Menaquinol biosynthetic pathways	2409:2440	Menaquinol biosynthetic pathways	2409:2440	Menaquinol biosynthetic pathways were associated with UC that belonged to <i>K. pneumoniae</i> strains.
36268225	3	128	theme	Bowel	951:955	arg1	Disease					957:963	the Inflammatory Bowel Disease	934:963	the Inflammatory Bowel Disease Multi'omics Database (IBDMDB)	934:993	Methods Metagenomic datasets were selected from the Integrative Human Microbiome Project (HMP2) through the Inflammatory Bowel Disease Multi'omics Database (IBDMDB).
36268225	5	129	used	used	1136:1139	arg2	We					1133:1134	We	1133:1134	We	1133:1134	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36268225	6	130	theme	<i>Escherichia	1479:1492	arg1	coli</i>					1494:1501	<i>Escherichia coli</i>	1479:1501	<i>Escherichia coli</i>	1479:1501	Results Compared to the gut microbiome of HCs, six Enterobacteriaceae species were significantly elevated in both CD and UC patients, including <i>Escherichia coli</i>, <i>Klebsiella variicola</i>, <i>Klebsiella quasipneumoniae</i>, <i>Klebsiella pneumoniae</i>, <i>Proteus mirabilis</i>, <i>Citrobacter freundii</i>, and <i>Citrobacter youngae</i>, while <i>Klebsiella oxytoca</i>, <i>Morganella morganii</i>, and <i>Citrobacter amalonaticus</i> were uniquely differentially abundant and enriched in the CD cohort.
36268225	1	131	theme	strands	241:247	arg1	number					220:225	A number	218:225	A number of converging strands of research	218:259	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	9	132	theme	nitrogen	2270:2277	arg1	metabolism					2279:2288	nitrogen metabolism	2270:2288	nitrogen metabolism	2270:2288	Biosynthetic pathways of aerobactin siderophore, LPS, enterobacterial common antigen, nitrogen metabolism, and sulfur relay systems encoded by <i>E. coli</i> were significantly elevated in the CD samples compared to the HCs.
36268225	1	133	theme	research	252:259	arg1	strands					241:247	converging strands	230:247	converging strands of research	230:259	Objectives A number of converging strands of research suggest that the intestinal Enterobacteriaceae plays a crucial role in the development and progression of inflammatory bowel disease (IBD), however, the changes in the abundance of Enterobacteriaceae species and their related metabolic pathways in Crohn's disease (CD) and ulcerative colitis (UC) compared to healthy people are not fully explained by comprehensive comparative metagenomics analysis.
36268225	8	134	theme	bacterial	2163:2171	arg1	community					2173:2181	their intestinal bacterial community	2146:2181	their intestinal bacterial community	2146:2181	Our analysis also showed a dramatically increased abundance of <i>E. coli</i> in their intestinal bacterial community.
36268225	5	135	theme	relative	1206:1213	arg1	abundance					1215:1223	relative abundance	1206:1223	relative abundance	1206:1223	We used the metagenomics dataset to study bacterial community structure, relative abundance, differentially abundant bacteria, functional analysis, and Enterobacteriaceae-related biosynthetic pathways.
36394293	8	0	theme	first	1295:1299	arg1	this					1283:1286	this	1283:1286	this	1283:1286	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	8	0	theme	first	1295:1299	arg1	report					1301:1306	the first report	1291:1306	the first report of nucleotide effector regulated β-N-acetylhexosaminidase	1291:1364	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	9	1	from	nm	1634:1635	arg1	densities					1617:1625	optical cell densities	1604:1625	optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1604:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	1	2	theme	glycosyl	178:185	arg1	family					197:202	the glycosyl hydrolase family GH20	174:207	the glycosyl hydrolase family GH20	174:207	β-N-acetylhexosaminidases (EC3.2.1.52), which belong to the glycosyl hydrolase family GH20, are important enzymes for oligosaccharides modification.
36394293	9	3	theme	optical	1604:1610	arg1	densities					1617:1625	optical cell densities	1604:1625	optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1604:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	0	4	theme	β-N-acetylhexosaminidase	85:108	arg1	Am2136					110:115	Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	61:115	Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	61:115	Nucleotide binding as an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136.
36394293	4	5	theme	mucin	615:619	arg1	activity					637:644	the in vitro mucin glycan cleavage activity	602:644	the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136	602:698	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	1	6	theme	hydrolase	187:195	arg1	family					197:202	the glycosyl hydrolase family GH20	174:207	the glycosyl hydrolase family GH20	174:207	β-N-acetylhexosaminidases (EC3.2.1.52), which belong to the glycosyl hydrolase family GH20, are important enzymes for oligosaccharides modification.
36394293	4	7	theme	Am2136	693:698	arg1	activity					637:644	the in vitro mucin glycan cleavage activity	602:644	the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136	602:698	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	3	8	theme	intestinal	435:444	arg1	muciniphila					407:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	8	theme	intestinal	435:444	arg1	bacterium					456:464	an anaerobic intestinal commensal bacterium	422:464	an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation	422:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	9	theme	commensal	446:454	arg1	muciniphila					407:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	9	theme	commensal	446:454	arg1	bacterium					456:464	an anaerobic intestinal commensal bacterium	422:464	an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation	422:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	9	10	theme	GH20	1487:1490	arg1	family					1492:1497	the GH20 family	1483:1497	the GH20 family	1483:1497	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	11	theme	phenylmethanesulfonyl	1711:1731	arg1	fluoride					1733:1740	phenylmethanesulfonyl fluoride	1711:1740	phenylmethanesulfonyl fluoride	1711:1740	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	12	theme	controllable	1539:1550	arg1	catalysts.Abbreviations					1571:1593	catalysts.Abbreviations	1571:1593	controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1539:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	5	13	theme	inter-domain	1002:1013	arg1	interactions					1015:1026	the inter-domain interactions	998:1026	the inter-domain interactions	998:1026	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	5	14	theme	enzyme	918:923	arg1	assay					934:938	enzyme activity assay	918:938	enzyme activity assay	918:938	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	8	15	theme	effector	1322:1329	arg1	β-N-acetylhexosaminidase					1341:1364	nucleotide effector regulated β-N-acetylhexosaminidase	1311:1364	nucleotide effector regulated β-N-acetylhexosaminidase	1311:1364	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	4	16	theme	wide	794:797	arg1	variety					799:805	a wide variety	792:805	a wide variety	792:805	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	4	16	theme	wide	794:797	arg1	residues					819:826	aglycone residues	810:826	aglycone residues	810:826	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	3	17	theme	anaerobic	425:433	arg1	muciniphila					407:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	17	theme	anaerobic	425:433	arg1	bacterium					456:464	an anaerobic intestinal commensal bacterium	422:464	an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation	422:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	4	18	dep	A.	653:654	arg1	muciniphila					656:666	muciniphila	656:666	muciniphila	656:666	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	0	19	theme	regulatory	36:45	arg1	mechanism					47:55	an allosteric regulatory mechanism	22:55	an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	22:115	Nucleotide binding as an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136.
36394293	8	20	theme	regulated	1331:1339	arg1	β-N-acetylhexosaminidase					1341:1364	nucleotide effector regulated β-N-acetylhexosaminidase	1311:1364	nucleotide effector regulated β-N-acetylhexosaminidase	1311:1364	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	3	21	theme	mucin	556:560	arg1	degradation					562:572	mucin degradation	556:572	mucin degradation	556:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	8	22	theme	β-N-acetylhexosaminidase	1341:1364	arg1	this					1283:1286	this	1283:1286	this	1283:1286	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	8	22	theme	β-N-acetylhexosaminidase	1341:1364	arg1	report					1301:1306	the first report	1291:1306	the first report of nucleotide effector regulated β-N-acetylhexosaminidase	1291:1364	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	3	23	theme	Akkermansia	395:405	arg1	muciniphila					407:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	23	theme	Akkermansia	395:405	arg1	bacterium					456:464	an anaerobic intestinal commensal bacterium	422:464	an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation	422:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	9	24	theme	root	1750:1753	arg1	deviation					1767:1775	root mean square deviation	1750:1775	root mean square deviation	1750:1775	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	25	theme	mean	1755:1758	arg1	deviation					1767:1775	root mean square deviation	1750:1775	root mean square deviation	1750:1775	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	3	26	theme	layer	533:537	arg1	colonization					539:550	gut mucosal layer colonization	521:550	gut mucosal layer colonization	521:550	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	27	theme	specific	482:489	arg1	β-N-acetylhexosaminidases					491:515	specific β-N-acetylhexosaminidases	482:515	specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation	482:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	4	28	theme	A.	653:654	arg1	Am2136					693:698	the A. muciniphila β-N-acetylhexosaminidase Am2136	649:698	the A. muciniphila β-N-acetylhexosaminidase Am2136	649:698	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	9	29	theme	glycan	1552:1557	arg1	catalysts.Abbreviations					1571:1593	catalysts.Abbreviations	1571:1593	controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1539:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	30	theme	square	1760:1765	arg1	deviation					1767:1775	root mean square deviation	1750:1775	root mean square deviation	1750:1775	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	8	31	theme	nucleotide	1311:1320	arg1	β-N-acetylhexosaminidase					1341:1364	nucleotide effector regulated β-N-acetylhexosaminidase	1311:1364	nucleotide effector regulated β-N-acetylhexosaminidase	1311:1364	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	3	32	contain	possesses	472:480	arg1	muciniphila					407:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila	395:417	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	32	contain	possesses	472:480	arg1	bacterium					456:464	an anaerobic intestinal commensal bacterium	422:464	an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation	422:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	3	32	contain	possesses	472:480	arg2	β-N-acetylhexosaminidases					491:515	specific β-N-acetylhexosaminidases	482:515	specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation	482:572	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	1	33	theme	important	214:222	arg1	enzymes					224:230	important enzymes	214:230	important enzymes for oligosaccharides modification	214:264	β-N-acetylhexosaminidases (EC3.2.1.52), which belong to the glycosyl hydrolase family GH20, are important enzymes for oligosaccharides modification.
36394293	1	33	theme	important	214:222	arg1	β-N-acetylhexosaminidases					118:142	β-N-acetylhexosaminidases	118:142	β-N-acetylhexosaminidases (EC3.2.1.52)	118:155	β-N-acetylhexosaminidases (EC3.2.1.52), which belong to the glycosyl hydrolase family GH20, are important enzymes for oligosaccharides modification.
36394293	4	34	theme	β-N-acetylhexosaminidase	668:691	arg1	Am2136					693:698	the A. muciniphila β-N-acetylhexosaminidase Am2136	649:698	the A. muciniphila β-N-acetylhexosaminidase Am2136	649:698	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	4	35	theme	generalist	866:875	arg1	β-N-acetylhexosaminidase					877:900	a generalist β-N-acetylhexosaminidase	864:900	a generalist β-N-acetylhexosaminidase	864:900	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	4	35	theme	generalist	866:875	arg1	Am2136					850:855	Am2136	850:855	Am2136	850:855	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	9	36	theme	distinct	1456:1463	arg1	properties					1465:1474	the distinct properties	1452:1474	the distinct properties within the GH20 family	1452:1497	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	3	37	theme	gut	521:523	arg1	colonization					539:550	gut mucosal layer colonization	521:550	gut mucosal layer colonization	521:550	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	7	38	theme	conformational	1215:1228	arg1	motions					1230:1236	the conformational motions	1211:1236	the conformational motions between domain III and IV	1211:1262	We further speculated that this activation mechanism might be associated with the conformational motions between domain III and IV.
36394293	2	39	theme	microbial	276:284	arg1	β-N-acetylhexosaminidases					286:310	Numerous microbial β-N-acetylhexosaminidases	267:310	Numerous microbial β-N-acetylhexosaminidases	267:310	Numerous microbial β-N-acetylhexosaminidases have been investigated for applications in biology, biomedicine and biotechnology.
36394293	9	40	theme	certain	1509:1515	arg1	foundation					1517:1526	a certain foundation	1507:1526	a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1507:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	0	41	theme	allosteric	25:34	arg1	mechanism					47:55	an allosteric regulatory mechanism	22:55	an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	22:115	Nucleotide binding as an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136.
36394293	4	42	theme	cleavage	628:635	arg1	activity					637:644	the in vitro mucin glycan cleavage activity	602:644	the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136	602:698	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	3	43	theme	mucosal	525:531	arg1	colonization					539:550	gut mucosal layer colonization	521:550	gut mucosal layer colonization	521:550	Akkermansia muciniphila is an anaerobic intestinal commensal bacterium which possesses specific β-N-acetylhexosaminidases for gut mucosal layer colonization and mucin degradation.
36394293	9	44	dep	catalysts.Abbreviations	1571:1593	arg1	OD600					1596:1600	OD600	1596:1600	OD600	1596:1600	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	44	dep	catalysts.Abbreviations	1571:1593	arg1	densities					1617:1625	optical cell densities	1604:1625	optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1604:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	2	45	theme	Numerous	267:274	arg1	β-N-acetylhexosaminidases					286:310	Numerous microbial β-N-acetylhexosaminidases	267:310	Numerous microbial β-N-acetylhexosaminidases	267:310	Numerous microbial β-N-acetylhexosaminidases have been investigated for applications in biology, biomedicine and biotechnology.
36394293	1	46	theme	oligosaccharides	236:251	arg1	modification					253:264	oligosaccharides modification	236:264	oligosaccharides modification	236:264	β-N-acetylhexosaminidases (EC3.2.1.52), which belong to the glycosyl hydrolase family GH20, are important enzymes for oligosaccharides modification.
36394293	8	47	theme	biological	1387:1396	arg1	functions					1398:1406	its novel biological functions	1377:1406	its novel biological functions	1377:1406	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	4	48	theme	glycan	621:626	arg1	activity					637:644	the in vitro mucin glycan cleavage activity	602:644	the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136	602:698	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	5	49	theme	Structural	903:912	arg1	experiments					940:950	Structural and enzyme activity assay experiments	903:950	Structural and enzyme activity assay experiments	903:950	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	8	50	theme	novel	1381:1385	arg1	functions					1398:1406	its novel biological functions	1377:1406	its novel biological functions	1377:1406	To our knowledge, this is the first report of nucleotide effector regulated β-N-acetylhexosaminidase, to reveal its novel biological functions.
36394293	4	51	dep	in	606:607	arg1	vitro					609:613	vitro	609:613	vitro	609:613	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	6	52	theme	hydrolysis	1074:1083	arg1	activity					1085:1092	the hydrolysis activity	1070:1092	the hydrolysis activity of Am2136	1070:1102	Importantly, we revealed that the hydrolysis activity of Am2136 was enhanced by nucleotides.
36394293	9	53	dep	nm	1634:1635	arg1	N-acetyl-β-D-glucosamine					1787:1810	N-acetyl-β-D-glucosamine	1787:1810	N-acetyl-β-D-glucosamine	1787:1810	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	Gal					1850:1852	Gal	1850:1852	Gal	1850:1852	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	fluoride					1733:1740	phenylmethanesulfonyl fluoride	1711:1740	phenylmethanesulfonyl fluoride	1711:1740	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	galactose					1856:1864	galactose	1856:1864	600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1630:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	LB					1638:1639	LB	1638:1639	600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1630:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	deviation					1767:1775	root mean square deviation	1750:1775	root mean square deviation	1750:1775	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	GalNAc					1813:1818	GalNAc	1813:1818	GalNAc	1813:1818	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	β-D-1-thiogalactopyranoside					1675:1701	isopropyl β-D-1-thiogalactopyranoside	1665:1701	isopropyl β-D-1-thiogalactopyranoside	1665:1701	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	N-acetyl-β-D-galactosamine					1822:1847	N-acetyl-β-D-galactosamine	1822:1847	N-acetyl-β-D-galactosamine	1822:1847	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	rmsd					1743:1746	rmsd	1743:1746	rmsd	1743:1746	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	Luria-Bertani					1643:1655	Luria-Bertani	1643:1655	Luria-Bertani	1643:1655	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	GlcNAc					1778:1783	GlcNAc	1778:1783	GlcNAc	1778:1783	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	PMSF					1704:1707	PMSF	1704:1707	PMSF	1704:1707	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	53	dep	nm	1634:1635	arg1	IPTG					1658:1661	IPTG	1658:1661	IPTG	1658:1661	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	9	54	theme	isopropyl	1665:1673	arg1	β-D-1-thiogalactopyranoside					1675:1701	isopropyl β-D-1-thiogalactopyranoside	1665:1701	isopropyl β-D-1-thiogalactopyranoside	1665:1701	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	2	55	from	applications	339:350	arg1	biomedicine					364:374	biomedicine	364:374	biomedicine	364:374	Numerous microbial β-N-acetylhexosaminidases have been investigated for applications in biology, biomedicine and biotechnology.
36394293	2	55	from	applications	339:350	arg1	biology					355:361	biology	355:361	biology	355:361	Numerous microbial β-N-acetylhexosaminidases have been investigated for applications in biology, biomedicine and biotechnology.
36394293	2	55	from	applications	339:350	arg1	biotechnology					380:392	biotechnology	380:392	biotechnology	380:392	Numerous microbial β-N-acetylhexosaminidases have been investigated for applications in biology, biomedicine and biotechnology.
36394293	9	56	theme	hydrolyzing	1559:1569	arg1	catalysts.Abbreviations					1571:1593	catalysts.Abbreviations	1571:1593	controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1539:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	5	57	from	function	986:993	arg1	β23-β33					1031:1037	β23-β33	1031:1037	β23-β33	1031:1037	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	5	58	theme	activity	925:932	arg1	assay					934:938	enzyme activity assay	918:938	enzyme activity assay	918:938	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	9	59	theme	cell	1612:1615	arg1	densities					1617:1625	optical cell densities	1604:1625	optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose	1604:1864	These findings contribute to understanding the distinct properties within the GH20 family and lay a certain foundation to develop controllable glycan hydrolyzing catalysts.Abbreviations: OD600 - optical cell densities at 600 nm; LB - Luria-Bertani; IPTG - isopropyl β-D-1-thiogalactopyranoside; PMSF - phenylmethanesulfonyl fluoride; rmsd - root mean square deviation; GlcNAc - N-acetyl-β-D-glucosamine; GalNAc - N-acetyl-β-D-galactosamine; Gal - galactose.
36394293	0	60	theme	muciniphila	73:83	arg1	Am2136					110:115	Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	61:115	Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	61:115	Nucleotide binding as an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136.
36394293	7	61	theme	activation	1165:1174	arg1	mechanism					1176:1184	this activation mechanism	1160:1184	this activation mechanism	1160:1184	We further speculated that this activation mechanism might be associated with the conformational motions between domain III and IV.
36394293	5	62	theme	essential	976:984	arg1	function					986:993	the essential function	972:993	the essential function of the inter-domain interactions in β23-β33	972:1037	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	4	63	theme	residues	819:826	arg1	variety					799:805	a wide variety	792:805	a wide variety	792:805	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	4	63	theme	residues	819:826	arg1	residues					819:826	aglycone residues	810:826	aglycone residues	810:826	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	5	64	theme	interactions	1015:1026	arg1	function					986:993	the essential function	972:993	the essential function of the inter-domain interactions in β23-β33	972:1037	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	0	65	theme	Akkermansia	61:71	arg1	Am2136					110:115	Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	61:115	Akkermansia muciniphila β-N-acetylhexosaminidase Am2136	61:115	Nucleotide binding as an allosteric regulatory mechanism for Akkermansia muciniphila β-N-acetylhexosaminidase Am2136.
36394293	4	66	theme	in	606:607	arg1	activity					637:644	the in vitro mucin glycan cleavage activity	602:644	the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136	602:698	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	5	67	theme	assay	934:938	arg1	experiments					940:950	Structural and enzyme activity assay experiments	903:950	Structural and enzyme activity assay experiments	903:950	Structural and enzyme activity assay experiments allowed us to probe the essential function of the inter-domain interactions in β23-β33.
36394293	4	68	theme	aglycone	810:817	arg1	residues					819:826	aglycone residues	810:826	aglycone residues	810:826	In this study, we assessed the in vitro mucin glycan cleavage activity of the A. muciniphila β-N-acetylhexosaminidase Am2136 and demonstrated its ability that hydrolyzing the β-linkages joining N-acetylglucosamine to a wide variety of aglycone residues, which indicated that Am2136 may be a generalist β-N-acetylhexosaminidase.
36394293	6	69	theme	Am2136	1097:1102	arg1	activity					1085:1092	the hydrolysis activity	1070:1092	the hydrolysis activity of Am2136	1070:1102	Importantly, we revealed that the hydrolysis activity of Am2136 was enhanced by nucleotides.
36961602	6	0	theme	strain	551:556	arg1	FJAT-45399T					558:568	strain FJAT-45399T	551:568	strain FJAT-45399T	551:568	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	2	1	theme	salt	110:113	arg1	FJAT-45399T					154:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	4	2	theme	16S	325:327	arg1	similarities					348:359	high 16S rRNA gene sequence similarities	320:359	high 16S rRNA gene sequence similarities	320:359	It shared high 16S rRNA gene sequence similarities with the members of the genus Shouchella.
36961602	11	3	theme	DSM	1155:1157	arg1	8716T					1159:1163	the most closely related type strain Shouchella clausii DSM 8716T	1099:1163	the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	1099:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	4	4	theme	Shouchella	391:400	arg1	members					370:376	the members	366:376	the members of the genus Shouchella	366:400	It shared high 16S rRNA gene sequence similarities with the members of the genus Shouchella.
36961602	5	5	theme	phylogenetic	416:427	arg1	analysis					446:453	the phylogenetic and phylogenomic analysis	412:453	analysis	446:453	Further, the phylogenetic and phylogenomic analysis also suggested strain FJAT-45399T clustered with the members of the genus Shouchella.
36961602	2	6	theme	alkali	102:107	arg1	FJAT-45399T					154:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	4	7	theme	genus	385:389	arg1	Shouchella					391:400	the genus Shouchella	381:400	the genus Shouchella	381:400	It shared high 16S rRNA gene sequence similarities with the members of the genus Shouchella.
36961602	1	8	theme	anaerobic	47:55	arg1	bacterium					57:65	a facultative anaerobic bacterium	33:65	a facultative anaerobic bacterium	33:65	nov., a facultative anaerobic bacterium isolated from marine sediments.
36961602	1	8	theme	anaerobic	47:55	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a facultative anaerobic bacterium isolated from marine sediments.
36961602	12	9	theme	novel	1311:1315	arg1	species					1317:1323	a novel species	1309:1323	a novel species	1309:1323	Based on the above results, strain FJAT-45399T represents a novel species of the genus Shouchella, for which the name Shouchella tritolerans sp.
36961602	4	10	theme	high	320:323	arg1	similarities					348:359	high 16S rRNA gene sequence similarities	320:359	high 16S rRNA gene sequence similarities	320:359	It shared high 16S rRNA gene sequence similarities with the members of the genus Shouchella.
36961602	2	11	theme	designated	143:152	arg1	FJAT-45399T					154:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	11	12	theme	strain	1076:1081	arg1	FJAT-45399T					1083:1093	strain FJAT-45399T	1076:1093	strain FJAT-45399T	1076:1093	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	13	theme	nucleotide	994:1003	arg1	ANI					1015:1017	ANI	1015:1017	ANI	1015:1017	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	13	theme	nucleotide	994:1003	arg1	identity					1005:1012	average nucleotide identity	986:1012	average nucleotide identity (ANI)	986:1018	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	8	14	theme	unidentified	799:810	arg1	glycolipid					812:821	an unidentified glycolipid	796:821	an unidentified glycolipid (UGL)	796:827	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and an unidentified glycolipid (UGL) and lipid (UL).
36961602	8	14	theme	unidentified	799:810	arg1	UGL					824:826	UGL	824:826	UGL	824:826	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and an unidentified glycolipid (UGL) and lipid (UL).
36961602	9	15	theme	fatty	855:859	arg1	%					872:872	> 10%	868:872	> 10%	868:872	The major fatty acids (> 10%) were C16:0 (22.8%), iso-C15:0 (21.3%), and anteiso-C15:0 (14.0%).
36961602	9	15	theme	fatty	855:859	arg1	C16:0					880:884	C16:0	880:884	C16:0 (22.8%)	880:892	The major fatty acids (> 10%) were C16:0 (22.8%), iso-C15:0 (21.3%), and anteiso-C15:0 (14.0%).
36961602	9	15	theme	fatty	855:859	arg1	acids					861:865	The major fatty acids	845:865	The major fatty acids (> 10%)	845:873	The major fatty acids (> 10%) were C16:0 (22.8%), iso-C15:0 (21.3%), and anteiso-C15:0 (14.0%).
36961602	2	16	theme	strain	136:141	arg1	FJAT-45399T					154:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	6	17	theme	optimum	663:669	arg1	%					672:672	optimum 2%	663:672	optimum 2%	663:672	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	17	theme	optimum	663:669	arg1	NaCl					657:660	0-15% (w/v) NaCl	645:660	0-15% (w/v) NaCl (optimum 2%)	645:673	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	2	18	theme	thermo-tolerant	120:134	arg1	strain					136:141	thermo-tolerant strain	120:141	thermo-tolerant strain	120:141	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	11	19	theme	related	1116:1122	arg1	8716T					1159:1163	the most closely related type strain Shouchella clausii DSM 8716T	1099:1163	the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	1099:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	20	theme	Shouchella	1136:1145	arg1	8716T					1159:1163	the most closely related type strain Shouchella clausii DSM 8716T	1099:1163	the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	1099:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	3	21	theme	Strain	227:232	arg1	FJAT-45399T					234:244	Strain FJAT-45399T	227:244	Strain FJAT-45399T	227:244	Strain FJAT-45399T was Gram-stain-positive, rod-shaped, and facultatively aerobic.
36961602	12	22	theme	name	1364:1367	arg1	Shouchella					1369:1378	the name Shouchella	1360:1378	the name Shouchella	1360:1378	Based on the above results, strain FJAT-45399T represents a novel species of the genus Shouchella, for which the name Shouchella tritolerans sp.
36961602	1	23	theme	marine	81:86	arg1	sediments					88:96	marine sediments	81:96	marine sediments	81:96	nov., a facultative anaerobic bacterium isolated from marine sediments.
36961602	11	24	theme	clausii	1147:1153	arg1	8716T					1159:1163	the most closely related type strain Shouchella clausii DSM 8716T	1099:1163	the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	1099:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	10	25	theme	G + C	957:961	arg1	content					963:969	The genomic DNA G + C content	941:969	The genomic DNA G + C content	941:969	The genomic DNA G + C content was 44.5%.
36961602	10	25	theme	G + C	957:961	arg1	%					979:979	44.5%	975:979	44.5%	975:979	The genomic DNA G + C content was 44.5%.
36961602	14	26	theme	type	1419:1422	arg1	FJAT-45399T					1434:1444	FJAT-45399T	1434:1444	FJAT-45399T (= GDMCC 1.3098T = JCM 35613T)	1434:1475	The type strain is FJAT-45399T (= GDMCC 1.3098T = JCM 35613T).
36961602	14	26	theme	type	1419:1422	arg1	strain					1424:1429	The type strain	1415:1429	The type strain	1415:1429	The type strain is FJAT-45399T (= GDMCC 1.3098T = JCM 35613T).
36961602	5	27	theme	strain	470:475	arg1	FJAT-45399T					477:487	strain FJAT-45399T	470:487	strain FJAT-45399T	470:487	Further, the phylogenetic and phylogenomic analysis also suggested strain FJAT-45399T clustered with the members of the genus Shouchella.
36961602	14	28	theme	1.3098T = JCM	1455:1467	arg1	FJAT-45399T					1434:1444	FJAT-45399T	1434:1444	FJAT-45399T (= GDMCC 1.3098T = JCM 35613T)	1434:1475	The type strain is FJAT-45399T (= GDMCC 1.3098T = JCM 35613T).
36961602	14	28	theme	1.3098T = JCM	1455:1467	arg1	35613T					1469:1474	= GDMCC 1.3098T = JCM 35613T	1447:1474	= GDMCC 1.3098T = JCM 35613T	1447:1474	The type strain is FJAT-45399T (= GDMCC 1.3098T = JCM 35613T).
36961602	11	29	theme	type	1124:1127	arg1	8716T					1159:1163	the most closely related type strain Shouchella clausii DSM 8716T	1099:1163	the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	1099:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	10	30	theme	DNA	953:955	arg1	content					963:969	The genomic DNA G + C content	941:969	The genomic DNA G + C content	941:969	The genomic DNA G + C content was 44.5%.
36961602	10	30	theme	DNA	953:955	arg1	%					979:979	44.5%	975:979	44.5%	975:979	The genomic DNA G + C content was 44.5%.
36961602	11	31	theme	DNA-DNA	1032:1038	arg1	dDDH					1055:1058	dDDH	1055:1058	dDDH	1055:1058	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	31	theme	DNA-DNA	1032:1038	arg1	hybridization					1040:1052	digital DNA-DNA hybridization	1024:1052	digital DNA-DNA hybridization (dDDH)	1024:1059	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	32	theme	identity	1005:1012	arg1	both					1197:1200	both	1197:1200	both	1197:1200	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	32	theme	identity	1005:1012	arg1	values					1061:1066	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	982:1066	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	982:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	2	33	attach	isolated	170:177	arg2	FJAT-45399T					154:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T	99:164	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	2	33	attach	isolated	170:177	arg1	sediment					191:198	marine sediment	184:198	marine sediment	184:198	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	6	34	theme	optimum	596:602	arg1	15-55 °C					586:593	15-55 °C	586:593	15-55 °C (optimum 45-50 °C)	586:612	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	34	theme	optimum	596:602	arg1	45-50 °C					604:611	optimum 45-50 °C	596:611	optimum 45-50 °C	596:611	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	10	35	theme	genomic	945:951	arg1	content					963:969	The genomic DNA G + C content	941:969	The genomic DNA G + C content	941:969	The genomic DNA G + C content was 44.5%.
36961602	10	35	theme	genomic	945:951	arg1	%					979:979	44.5%	975:979	44.5%	975:979	The genomic DNA G + C content was 44.5%.
36961602	11	36	theme	hybridization	1040:1052	arg1	both					1197:1200	both	1197:1200	both	1197:1200	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	36	theme	hybridization	1040:1052	arg1	values					1061:1066	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	982:1066	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	982:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	37	theme	digital	1024:1030	arg1	dDDH					1055:1058	dDDH	1055:1058	dDDH	1055:1058	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	37	theme	digital	1024:1030	arg1	hybridization					1040:1052	digital DNA-DNA hybridization	1024:1052	digital DNA-DNA hybridization (dDDH)	1024:1059	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	38	theme	species	1230:1236	arg1	delineation					1238:1248	species delineation	1230:1248	species delineation	1230:1248	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	39	theme	cut-off	1212:1218	arg1	level					1220:1224	the cut-off level	1208:1224	the cut-off level for species delineation	1208:1248	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	12	40	theme	above	1264:1268	arg1	results					1270:1276	the above results	1260:1276	the above results	1260:1276	Based on the above results, strain FJAT-45399T represents a novel species of the genus Shouchella, for which the name Shouchella tritolerans sp.
36961602	6	41	theme	%	649:649	arg1	%					672:672	optimum 2%	663:672	optimum 2%	663:672	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	41	theme	%	649:649	arg1	NaCl					657:660	0-15% (w/v) NaCl	645:660	0-15% (w/v) NaCl (optimum 2%)	645:673	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	42	theme	0-15	645:648	arg1	%					649:649	%	649:649	%	649:649	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	11	43	theme	ANI	1166:1168	arg1	%					1174:1174	ANI 94.1%	1166:1174	ANI 94.1%	1166:1174	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	12	44	theme	strain	1279:1284	arg1	FJAT-45399T					1286:1296	strain FJAT-45399T	1279:1296	strain FJAT-45399T	1279:1296	Based on the above results, strain FJAT-45399T represents a novel species of the genus Shouchella, for which the name Shouchella tritolerans sp.
36961602	12	45	theme	genus	1332:1336	arg1	Shouchella					1338:1347	the genus Shouchella	1328:1347	the genus Shouchella	1328:1347	Based on the above results, strain FJAT-45399T represents a novel species of the genus Shouchella, for which the name Shouchella tritolerans sp.
36961602	4	46	theme	sequence	339:346	arg1	similarities					348:359	high 16S rRNA gene sequence similarities	320:359	high 16S rRNA gene sequence similarities	320:359	It shared high 16S rRNA gene sequence similarities with the members of the genus Shouchella.
36961602	11	47	theme	strain	1129:1134	arg1	8716T					1159:1163	the most closely related type strain Shouchella clausii DSM 8716T	1099:1163	the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%)	1099:1190	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	12	48	theme	Shouchella	1338:1347	arg1	species					1317:1323	a novel species	1309:1323	a novel species	1309:1323	Based on the above results, strain FJAT-45399T represents a novel species of the genus Shouchella, for which the name Shouchella tritolerans sp.
36961602	11	49	theme	dDDH	1180:1183	arg1	%					1189:1189	dDDH 55.4%	1180:1189	dDDH 55.4%	1180:1189	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	4	50	theme	gene	334:337	arg1	similarities					348:359	high 16S rRNA gene sequence similarities	320:359	high 16S rRNA gene sequence similarities	320:359	It shared high 16S rRNA gene sequence similarities with the members of the genus Shouchella.
36961602	3	51	dep	Gram-stain-positive	250:268	arg1	aerobic					301:307	aerobic	301:307	aerobic	301:307	Strain FJAT-45399T was Gram-stain-positive, rod-shaped, and facultatively aerobic.
36961602	11	52	dep	8716T	1159:1163	arg1	%					1189:1189	dDDH 55.4%	1180:1189	dDDH 55.4%	1180:1189	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	52	dep	8716T	1159:1163	arg1	%					1174:1174	ANI 94.1%	1166:1174	ANI 94.1%	1166:1174	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	6	53	located	observed	574:581	arg1	15-55 °C					586:593	15-55 °C	586:593	15-55 °C (optimum 45-50 °C)	586:612	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	53	located	observed	574:581	arg1	pH					615:616	pH 7.0-13.0	615:625	pH 7.0-13.0 (optimum 9.0)	615:639	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	53	located	observed	574:581	arg1	optimum					628:634	optimum 9.0	628:638	optimum 9.0	628:638	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	53	located	observed	574:581	arg2	Growth					541:546	Growth	541:546	Growth of strain FJAT-45399T	541:568	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	53	located	observed	574:581	arg1	45-50 °C					604:611	optimum 45-50 °C	596:611	optimum 45-50 °C	596:611	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	53	located	observed	574:581	arg1	%					672:672	optimum 2%	663:672	optimum 2%	663:672	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	6	53	located	observed	574:581	arg1	NaCl					657:660	0-15% (w/v) NaCl	645:660	0-15% (w/v) NaCl (optimum 2%)	645:673	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	14	54	theme	= GDMCC	1447:1453	arg1	FJAT-45399T					1434:1444	FJAT-45399T	1434:1444	FJAT-45399T (= GDMCC 1.3098T = JCM 35613T)	1434:1475	The type strain is FJAT-45399T (= GDMCC 1.3098T = JCM 35613T).
36961602	14	54	theme	= GDMCC	1447:1453	arg1	35613T					1469:1474	= GDMCC 1.3098T = JCM 35613T	1447:1474	= GDMCC 1.3098T = JCM 35613T	1447:1474	The type strain is FJAT-45399T (= GDMCC 1.3098T = JCM 35613T).
36961602	5	55	theme	phylogenomic	433:444	arg1	analysis					446:453	the phylogenetic and phylogenomic analysis	412:453	analysis	446:453	Further, the phylogenetic and phylogenomic analysis also suggested strain FJAT-45399T clustered with the members of the genus Shouchella.
36961602	5	56	dep	suggested	460:468	arg1	clustered					489:497	clustered	489:497	suggested strain FJAT-45399T clustered with the members of the genus Shouchella	460:538	Further, the phylogenetic and phylogenomic analysis also suggested strain FJAT-45399T clustered with the members of the genus Shouchella.
36961602	6	57	dep	%	649:649	arg1	w/v					652:654	w/v	652:654	w/v	652:654	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	7	58	contain	contained	679:687	arg2	menaquinone					701:711	the menaquinone	697:711	the menaquinone	697:711	It contained MK-7 as the menaquinone.
36961602	7	58	contain	contained	679:687	arg2	MK-7					689:692	MK-7	689:692	MK-7	689:692	It contained MK-7 as the menaquinone.
36961602	7	58	contain	contained	679:687	arg1	It					676:677	It	676:677	It	676:677	It contained MK-7 as the menaquinone.
36961602	5	59	theme	genus	523:527	arg1	Shouchella					529:538	the genus Shouchella	519:538	the genus Shouchella	519:538	Further, the phylogenetic and phylogenomic analysis also suggested strain FJAT-45399T clustered with the members of the genus Shouchella.
36961602	11	60	theme	average	986:992	arg1	ANI					1015:1017	ANI	1015:1017	ANI	1015:1017	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	11	60	theme	average	986:992	arg1	identity					1005:1012	average nucleotide identity	986:1012	average nucleotide identity (ANI)	986:1018	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain FJAT-45399T and the most closely related type strain Shouchella clausii DSM 8716T (ANI 94.1% and dDDH 55.4%) were both below the cut-off level for species delineation.
36961602	2	61	theme	marine	184:189	arg1	sediment					191:198	marine sediment	184:198	marine sediment	184:198	An alkali, salt, and thermo-tolerant strain designated FJAT-45399T was isolated from marine sediment in Fujian Province, China.
36961602	5	62	theme	Shouchella	529:538	arg1	members					508:514	the members	504:514	the members of the genus Shouchella	504:538	Further, the phylogenetic and phylogenomic analysis also suggested strain FJAT-45399T clustered with the members of the genus Shouchella.
36961602	8	63	theme	polar	718:722	arg1	diphosphatidylglycerol					736:757	diphosphatidylglycerol	736:757	diphosphatidylglycerol (DPG)	736:763	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and an unidentified glycolipid (UGL) and lipid (UL).
36961602	8	63	theme	polar	718:722	arg1	lipids					724:729	The polar lipids	714:729	The polar lipids	714:729	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and an unidentified glycolipid (UGL) and lipid (UL).
36961602	6	64	theme	FJAT-45399T	558:568	arg1	Growth					541:546	Growth	541:546	Growth of strain FJAT-45399T	541:568	Growth of strain FJAT-45399T was observed at 15-55 °C (optimum 45-50 °C), pH 7.0-13.0 (optimum 9.0) and 0-15% (w/v) NaCl (optimum 2%).
36961602	1	65	theme	facultative	35:45	arg1	bacterium					57:65	a facultative anaerobic bacterium	33:65	a facultative anaerobic bacterium	33:65	nov., a facultative anaerobic bacterium isolated from marine sediments.
36961602	1	65	theme	facultative	35:45	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a facultative anaerobic bacterium isolated from marine sediments.
36961602	4	66	theme	rRNA	329:332	arg1	similarities					348:359	high 16S rRNA gene sequence similarities	320:359	high 16S rRNA gene sequence similarities	320:359	It shared high 16S rRNA gene sequence similarities with the members of the genus Shouchella.
36961602	9	67	theme	major	849:853	arg1	%					872:872	> 10%	868:872	> 10%	868:872	The major fatty acids (> 10%) were C16:0 (22.8%), iso-C15:0 (21.3%), and anteiso-C15:0 (14.0%).
36961602	9	67	theme	major	849:853	arg1	C16:0					880:884	C16:0	880:884	C16:0 (22.8%)	880:892	The major fatty acids (> 10%) were C16:0 (22.8%), iso-C15:0 (21.3%), and anteiso-C15:0 (14.0%).
36961602	9	67	theme	major	849:853	arg1	acids					861:865	The major fatty acids	845:865	The major fatty acids (> 10%)	845:873	The major fatty acids (> 10%) were C16:0 (22.8%), iso-C15:0 (21.3%), and anteiso-C15:0 (14.0%).
35834986	0	0	theme	colorectal	90:99	arg1	cancer					101:106	colitis-associated colorectal cancer	71:106	colitis-associated colorectal cancer	71:106	Cortex periplocae modulates the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells.
35834986	4	1	theme	PePs	741:744	arg1	treatment					746:754	PePs treatment	741:754	PePs treatment	741:754	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	1	2	theme	cell	174:177	arg1	factor					209:214	a predisposing factor	194:214	a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC)	194:286	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	2	theme	cell	174:177	arg1	interaction					179:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	3	theme	colitis-associated	256:273	arg1	cancer					275:280	colitis-associated cancer	256:280	colitis-associated cancer (CAC)	256:286	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	3	theme	colitis-associated	256:273	arg1	CAC					283:285	CAC	283:285	CAC	283:285	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	0	4	theme	colitis-associated	71:88	arg1	cancer					101:106	colitis-associated colorectal cancer	71:106	colitis-associated colorectal cancer	71:106	Cortex periplocae modulates the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells.
35834986	6	5	theme	microbiota	1150:1159	arg1	PePs					1114:1117	PePs	1114:1117	PePs	1114:1117	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	6	5	theme	microbiota	1150:1159	arg1	modulator					1161:1169	a potential gut microbiota modulator	1134:1169	a potential gut microbiota modulator to treat IBD and CAC	1134:1190	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	2	6	theme	anti-rheumatoid	362:376	arg1	arthritis					378:386	putative anti-rheumatoid arthritis	353:386	putative anti-rheumatoid arthritis	353:386	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	6	7	theme	gut	1146:1148	arg1	PePs					1114:1117	PePs	1114:1117	PePs	1114:1117	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	6	7	theme	gut	1146:1148	arg1	modulator					1161:1169	a potential gut microbiota modulator	1134:1169	a potential gut microbiota modulator to treat IBD and CAC	1134:1190	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	3	8	theme	root	536:539	arg1	bark					541:544	root bark	536:544	root bark of Cortex Periplocae	536:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	9	theme	Periplocae	556:565	arg1	bark					541:544	root bark	536:544	root bark of Cortex Periplocae	536:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	5	10	theme	Type	1034:1037	arg1	immunity					1042:1049	intestinal Type 17 immunity	1023:1049	intestinal Type 17 immunity	1023:1049	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	3	11	from	tumorigenesis	680:692	arg1	mice					697:700	mice	697:700	mice with colitis and CAC	697:721	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	5	12	theme	immunity	1042:1049	arg1	modulation					1009:1018	modulation	1009:1018	modulation of intestinal Type 17 immunity	1009:1049	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	2	13	theme	Cortex	289:294	arg1	Periplocae					296:305	Cortex Periplocae	289:305	Cortex Periplocae	289:305	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	2	13	theme	Cortex	289:294	arg1	medicine					339:346	a famous traditional Chinese medicine	310:346	a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects	310:413	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	3	14	theme	glycosides-free	487:501	arg1	glycosides					512:521	cardiac glycosides-free pregnane glycosides	479:521	a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae	477:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	15	theme	pregnane	503:510	arg1	glycosides					512:521	cardiac glycosides-free pregnane glycosides	479:521	a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae	477:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	16	from	bark	541:544	arg1	periplosides					456:467	the Periploca sepium periplosides	435:467	the Periploca sepium periplosides (PePs)	435:474	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	16	from	bark	541:544	arg1	extract					523:529	a cardiac glycosides-free pregnane glycosides extract	477:529	a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae	477:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	17	theme	Periploca	439:447	arg1	periplosides					456:467	the Periploca sepium periplosides	435:467	the Periploca sepium periplosides (PePs)	435:474	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	17	theme	Periploca	439:447	arg1	PePs					470:473	PePs	470:473	PePs	470:473	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	17	theme	Periploca	439:447	arg1	extract					523:529	a cardiac glycosides-free pregnane glycosides extract	477:529	a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae	477:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	18	theme	Cortex	549:554	arg1	Periplocae					556:565	Cortex Periplocae	549:565	Cortex Periplocae	549:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	6	19	theme	potential	1136:1144	arg1	PePs					1114:1117	PePs	1114:1117	PePs	1114:1117	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	6	19	theme	potential	1136:1144	arg1	modulator					1161:1169	a potential gut microbiota modulator	1134:1169	a potential gut microbiota modulator to treat IBD and CAC	1134:1190	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	5	20	theme	altered	934:940	arg1	microbiota					946:955	the altered gut microbiota	930:955	the altered gut microbiota following PePs treatment	930:980	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	6	21	used	used	1126:1129	arg2	modulator					1161:1169	a potential gut microbiota modulator	1134:1169	a potential gut microbiota modulator to treat IBD and CAC	1134:1190	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	6	21	used	used	1126:1129	arg2	PePs					1114:1117	PePs	1114:1117	PePs	1114:1117	Our results indicate that PePs may be used as a potential gut microbiota modulator to treat IBD and CAC.
35834986	2	22	theme	traditional	319:329	arg1	Periplocae					296:305	Cortex Periplocae	289:305	Cortex Periplocae	289:305	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	2	22	theme	traditional	319:329	arg1	medicine					339:346	a famous traditional Chinese medicine	310:346	a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects	310:413	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	1	23	theme	predisposing	196:207	arg1	factor					209:214	a predisposing factor	194:214	a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC)	194:286	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	23	theme	predisposing	196:207	arg1	interaction					179:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	0	24	theme	Cortex	0:5	arg1	periplocae					7:16	Cortex periplocae	0:16	Cortex periplocae	0:16	Cortex periplocae modulates the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells.
35834986	4	25	theme	abnormal	766:773	arg1	composition					790:800	abnormal gut microbiota composition	766:800	abnormal gut microbiota composition in model mice	766:814	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	4	26	from	composition	790:800	arg1	mice					811:814	model mice	805:814	model mice	805:814	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	2	27	theme	famous	312:317	arg1	Periplocae					296:305	Cortex Periplocae	289:305	Cortex Periplocae	289:305	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	2	27	theme	famous	312:317	arg1	medicine					339:346	a famous traditional Chinese medicine	310:346	a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects	310:413	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	3	28	theme	sepium	449:454	arg1	periplosides					456:467	the Periploca sepium periplosides	435:467	the Periploca sepium periplosides (PePs)	435:474	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	28	theme	sepium	449:454	arg1	PePs					470:473	PePs	470:473	PePs	470:473	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	28	theme	sepium	449:454	arg1	extract					523:529	a cardiac glycosides-free pregnane glycosides extract	477:529	a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae	477:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	5	29	theme	CAC	1071:1073	arg1	model					1081:1085	both colitis and CAC mouse model	1054:1085	model	1081:1085	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	0	30	theme	Th17	138:141	arg1	cells					143:147	pathogenic Th17 cells	127:147	pathogenic Th17 cells	127:147	Cortex periplocae modulates the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells.
35834986	4	31	theme	model	805:809	arg1	mice					811:814	model mice	805:814	model mice	805:814	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	4	32	theme	microbiota	779:788	arg1	composition					790:800	abnormal gut microbiota composition	766:800	abnormal gut microbiota composition in model mice	766:814	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	0	33	theme	pathogenic	127:136	arg1	cells					143:147	pathogenic Th17 cells	127:147	pathogenic Th17 cells	127:147	Cortex periplocae modulates the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells.
35834986	2	34	with	medicine	339:346	arg1	arthritis					378:386	putative anti-rheumatoid arthritis	353:386	putative anti-rheumatoid arthritis	353:386	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	2	34	with	medicine	339:346	arg1	effects					407:413	anti-dyspepsia effects	392:413	anti-dyspepsia effects	392:413	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	3	35	theme	colon	579:583	arg1	inflammation					585:596	colon inflammation	579:596	colon inflammation	579:596	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	1	36	theme	inflammatory	219:230	arg1	IBD					247:249	IBD	247:249	IBD	247:249	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	36	theme	inflammatory	219:230	arg1	disease					238:244	inflammatory bowel disease	219:244	inflammatory bowel disease (IBD)	219:250	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	5	37	theme	important	991:999	arg1	role					1001:1004	an important role	988:1004	an important role	988:1004	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	3	38	theme	epithelial	619:628	arg1	function					638:645	intestinal epithelial barrier function	608:645	intestinal epithelial barrier function	608:645	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	39	with	mice	697:700	arg1	colitis					707:713	colitis	707:713	colitis	707:713	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	39	with	mice	697:700	arg1	CAC					719:721	CAC	719:721	CAC	719:721	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	1	40	theme	bowel	232:236	arg1	IBD					247:249	IBD	247:249	IBD	247:249	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	40	theme	bowel	232:236	arg1	disease					238:244	inflammatory bowel disease	219:244	inflammatory bowel disease (IBD)	219:250	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	0	41	theme	gut	32:34	arg1	microbiota					36:45	the gut microbiota	28:45	the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells	28:147	Cortex periplocae modulates the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells.
35834986	0	42	theme	cells	143:147	arg1	suppression					112:122	suppression	112:122	suppression of pathogenic Th17 cells	112:147	Cortex periplocae modulates the gut microbiota to restrict colitis and colitis-associated colorectal cancer via suppression of pathogenic Th17 cells.
35834986	3	43	theme	colitis-associated	661:678	arg1	tumorigenesis					680:692	colitis-associated tumorigenesis	661:692	colitis-associated tumorigenesis in mice with colitis and CAC	661:721	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	44	theme	barrier	630:636	arg1	function					638:645	intestinal epithelial barrier function	608:645	intestinal epithelial barrier function	608:645	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	4	45	dep	A.	878:879	arg1	muciniphila					881:891	muciniphila	881:891	muciniphila	881:891	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	5	46	theme	gut	942:944	arg1	microbiota					946:955	the altered gut microbiota	930:955	the altered gut microbiota following PePs treatment	930:980	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	2	47	theme	putative	353:360	arg1	arthritis					378:386	putative anti-rheumatoid arthritis	353:386	putative anti-rheumatoid arthritis	353:386	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	5	48	theme	intestinal	1023:1032	arg1	immunity					1042:1049	intestinal Type 17 immunity	1023:1049	intestinal Type 17 immunity	1023:1049	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	3	49	theme	intestinal	608:617	arg1	function					638:645	intestinal epithelial barrier function	608:645	intestinal epithelial barrier function	608:645	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	50	theme	glycosides	512:521	arg1	periplosides					456:467	the Periploca sepium periplosides	435:467	the Periploca sepium periplosides (PePs)	435:474	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	3	50	theme	glycosides	512:521	arg1	extract					523:529	a cardiac glycosides-free pregnane glycosides extract	477:529	a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae	477:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	5	51	theme	mouse	1075:1079	arg1	model					1081:1085	both colitis and CAC mouse model	1054:1085	model	1081:1085	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	1	52	theme	Aberrant	150:157	arg1	factor					209:214	a predisposing factor	194:214	a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC)	194:286	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	52	theme	Aberrant	150:157	arg1	interaction					179:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	4	53	theme	anti-inflammatory	840:856	arg1	BAA-835					893:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	4	54	theme	A.	878:879	arg1	BAA-835					893:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	3	55	theme	cardiac	479:485	arg1	glycosides					512:521	cardiac glycosides-free pregnane glycosides	479:521	a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae	477:565	Here, we show that the Periploca sepium periplosides (PePs), a cardiac glycosides-free pregnane glycosides extract from root bark of Cortex Periplocae, alleviates colon inflammation, improves intestinal epithelial barrier function, and prevents colitis-associated tumorigenesis in mice with colitis and CAC.
35834986	4	56	dep	modulates	756:764	arg1	enriches					828:835	enriches	828:835	especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835	817:899	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	2	57	theme	Chinese	331:337	arg1	Periplocae					296:305	Cortex Periplocae	289:305	Cortex Periplocae	289:305	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	2	57	theme	Chinese	331:337	arg1	medicine					339:346	a famous traditional Chinese medicine	310:346	a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects	310:413	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	4	58	theme	gut	775:777	arg1	composition					790:800	abnormal gut microbiota composition	766:800	abnormal gut microbiota composition in model mice	766:814	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	5	59	theme	PePs	967:970	arg1	treatment					972:980	PePs treatment	967:980	PePs treatment	967:980	We further demonstrate that the altered gut microbiota following PePs treatment plays an important role in modulation of intestinal Type 17 immunity in both colitis and CAC mouse model.
35834986	4	60	theme	bacterium	868:876	arg1	BAA-835					893:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	1	61	from	factor	209:214	arg1	cancer					275:280	colitis-associated cancer	256:280	colitis-associated cancer (CAC)	256:286	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	61	from	factor	209:214	arg1	CAC					283:285	CAC	283:285	CAC	283:285	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	61	from	factor	209:214	arg1	IBD					247:249	IBD	247:249	IBD	247:249	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	61	from	factor	209:214	arg1	disease					238:244	inflammatory bowel disease	219:244	inflammatory bowel disease (IBD)	219:250	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	2	62	theme	anti-dyspepsia	392:405	arg1	effects					407:413	anti-dyspepsia effects	392:413	anti-dyspepsia effects	392:413	Cortex Periplocae is a famous traditional Chinese medicine with putative anti-rheumatoid arthritis and anti-dyspepsia effects.
35834986	4	63	theme	commensal	858:866	arg1	BAA-835					893:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	an anti-inflammatory commensal bacterium A. muciniphila BAA-835	837:899	Mechanistically, PePs treatment modulates abnormal gut microbiota composition in model mice, especially enriches an anti-inflammatory commensal bacterium A. muciniphila BAA-835.
35834986	1	64	theme	microbe-immune	159:172	arg1	factor					209:214	a predisposing factor	194:214	a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC)	194:286	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
35834986	1	64	theme	microbe-immune	159:172	arg1	interaction					179:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction	150:189	Aberrant microbe-immune cell interaction is a predisposing factor in inflammatory bowel disease (IBD) and colitis-associated cancer (CAC).
32563228	10	0	theme	different	1542:1550	arg1	models					1558:1563	the different mouse models	1538:1563	the different mouse models of allergic asthma created by the sensitization with different adjuvants	1538:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	4	1	theme	adjuvants	575:583	arg1	adjuvants					575:583	adjuvants	575:583	adjuvants	575:583	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	4	1	theme	adjuvants	575:583	arg1	variety					564:570	a variety	562:570	a variety of adjuvants	562:583	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	4	1	theme	adjuvants	575:583	arg1	characteristics					492:506	immunologic cellular characteristics	471:506	immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA)	471:556	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	4	2	from	characteristics	492:506	arg1	models					524:529	mouse asthma models	511:529	mouse asthma models induced by ovalbumin (OVA)	511:556	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	10	3	theme	allergic	1568:1575	arg1	asthma					1577:1582	allergic asthma	1568:1582	allergic asthma created by the sensitization with different adjuvants	1568:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	9	4	theme	ILCs	1296:1299	arg1	highest					1305:1311	highest	1305:1311	highest	1305:1311	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	9	4	theme	ILCs	1296:1299	arg1	number					1279:1284	the number	1275:1284	the number of T-bet+ ILCs	1275:1299	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	8	5	theme	LPS	1112:1114	arg1	Meanwhile					1097:1105	Meanwhile	1097:1105	Meanwhile	1097:1105	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	8	5	theme	LPS	1112:1114	arg1	group					1116:1120	the LPS group	1108:1120	the LPS group	1108:1120	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	8	6	theme	innate	1172:1177	arg1	ILCs					1195:1198	ILCs	1195:1198	ILCs	1195:1198	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	8	6	theme	innate	1172:1177	arg1	cells					1188:1192	RORγt+ innate lymphoid cells	1165:1192	RORγt+ innate lymphoid cells (ILCs)	1165:1199	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	10	7	dep	CONCLUSIONS	1401:1411	arg1	found					1435:1439	found	1435:1439	are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1431:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	4	8	theme	study	448:452	arg1	aim					437:439	The aim	433:439	The aim of our study	433:452	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	8	9	theme	cells	1188:1192	arg1	levels					1150:1155	the most elevated levels	1132:1155	the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells	1132:1220	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	5	10	theme	adjuvants	671:679	arg1	admixture					642:650	the admixture	638:650	the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG	638:747	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	2	11	theme	recent	209:214	arg1	studies					216:222	recent studies	209:222	recent studies	209:222	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	7	12	theme	alum	1018:1021	arg1	group					1023:1027	the alum group	1014:1027	the alum group	1014:1027	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	6	13	theme	lymph	872:876	arg1	mLN					884:886	mLN	884:886	mLN	884:886	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	6	13	theme	lymph	872:876	arg1	node					878:881	mediastinal lymph node	860:881	mediastinal lymph node (mLN)	860:887	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	7	14	theme	BAL	956:958	arg1	fluid					960:964	the lung and BAL fluid	943:964	fluid	960:964	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	9	15	theme	Th	1362:1363	arg1	cells					1365:1369	IFN-γ+ Th cells	1355:1369	IFN-γ+ Th cells	1355:1369	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	8	16	theme	Th	1213:1214	arg1	cells					1216:1220	IL-17A+ Th cells	1205:1220	IL-17A+ Th cells	1205:1220	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	0	17	theme	allergic	62:69	arg1	inflammation					78:89	allergic airway inflammation	62:89	allergic airway inflammation	62:89	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	4	18	theme	mouse	511:515	arg1	models					524:529	mouse asthma models	511:529	mouse asthma models induced by ovalbumin (OVA)	511:556	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	0	19	theme	inflammation	78:89	arg1	model					53:57	the mouse model	43:57	the mouse model of allergic airway inflammation	43:89	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	3	20	theme	cellular	338:345	arg1	phenotypes					347:356	cellular phenotypes	338:356	cellular phenotypes	338:356	OBJECTIVE We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants.
32563228	9	21	theme	CpG	1390:1392	arg1	group					1394:1398	the CpG group	1386:1398	the CpG group	1386:1398	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	4	22	theme	immunologic	471:481	arg1	characteristics					492:506	immunologic cellular characteristics	471:506	immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA)	471:556	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	8	23	theme	mediastinal	1238:1248	arg1	node					1256:1259	mediastinal lymph node	1238:1259	mediastinal lymph node	1238:1259	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	0	24	theme	Distinct	0:7	arg1	effects					9:15	Distinct effects	0:15	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation	0:89	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	3	25	theme	mouse	365:369	arg1	model					371:375	the mouse model	361:375	the mouse model of allergic asthma sensitized with different adjuvants	361:430	OBJECTIVE We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants.
32563228	1	26	theme	Allergic	103:110	arg1	asthma					112:117	BACKGROUND Allergic asthma	92:117	BACKGROUND Allergic asthma	92:117	BACKGROUND Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity.
32563228	1	26	theme	Allergic	103:110	arg1	disease					163:169	an inflammatory disease	147:169	an inflammatory disease mediated by type 2 immunity	147:197	BACKGROUND Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity.
32563228	7	27	theme	LPS	1086:1088	arg1	group					1090:1094	the LPS group	1082:1094	the LPS group	1082:1094	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	0	28	theme	adjuvants	30:38	arg1	effects					9:15	Distinct effects	0:15	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation	0:89	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	6	29	theme	bronchoalveolar	814:828	arg1	BAL					838:840	BAL	838:840	BAL	838:840	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	6	29	theme	bronchoalveolar	814:828	arg1	lavage					830:835	bronchoalveolar lavage	814:835	bronchoalveolar lavage (BAL) fluid	814:847	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	3	30	theme	asthma	389:394	arg1	model					371:375	the mouse model	361:375	the mouse model of allergic asthma sensitized with different adjuvants	361:430	OBJECTIVE We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants.
32563228	7	31	theme	neutrophil	1047:1056	arg1	levels					1058:1063	the highest neutrophil levels	1035:1063	the highest neutrophil levels	1035:1063	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	10	32	located	found	1435:1439	arg1	composition					1448:1458	the composition	1444:1458	the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1444:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	32	located	found	1435:1439	arg2	variances					1421:1429	Notable variances	1413:1429	Notable variances	1413:1429	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	33	theme	pathogenesis	1519:1530	arg1	pathogenesis					1519:1530	pathogenesis	1519:1530	pathogenesis	1519:1530	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	33	theme	pathogenesis	1519:1530	arg1	site					1511:1514	the site	1507:1514	the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1507:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	0	34	theme	mouse	47:51	arg1	model					53:57	the mouse model	43:57	the mouse model of allergic airway inflammation	43:89	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	6	35	theme	mediastinal	860:870	arg1	mLN					884:886	mLN	884:886	mLN	884:886	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	6	35	theme	mediastinal	860:870	arg1	node					878:881	mediastinal lymph node	860:881	mediastinal lymph node (mLN)	860:887	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	10	36	from	site	1511:1514	arg1	expression					1480:1489	expression	1480:1489	expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1480:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	36	from	site	1511:1514	arg1	composition					1448:1458	the composition	1444:1458	the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1444:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	36	from	site	1511:1514	arg1	cells					1470:1474	immune cells	1463:1474	immune cells	1463:1474	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	37	theme	mouse	1552:1556	arg1	models					1558:1563	the different mouse models	1538:1563	the different mouse models of allergic asthma created by the sensitization with different adjuvants	1538:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	38	theme	Notable	1413:1419	arg1	variances					1421:1429	Notable variances	1413:1429	Notable variances	1413:1429	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	5	39	theme	OVA	655:657	arg1	admixture					642:650	the admixture	638:650	the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG	638:747	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	2	40	theme	endotypes	305:313	arg1	phenotypes					290:299	phenotypes	290:299	phenotypes	290:299	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	2	40	theme	endotypes	305:313	arg1	variety					279:285	a variety	277:285	a variety of phenotypes and endotypes	277:313	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	2	40	theme	endotypes	305:313	arg1	endotypes					305:313	endotypes	305:313	endotypes	305:313	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	10	41	from	composition	1448:1458	arg1	pathogenesis					1519:1530	pathogenesis	1519:1530	pathogenesis	1519:1530	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	41	from	composition	1448:1458	arg1	site					1511:1514	the site	1507:1514	the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1507:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	9	42	theme	cells	1365:1369	arg1	number					1345:1350	the number	1341:1350	the number of IFN-γ+ Th cells	1341:1369	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	9	42	theme	cells	1365:1369	arg1	highest					1375:1381	highest	1375:1381	highest	1375:1381	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	10	43	theme	asthma	1577:1582	arg1	models					1558:1563	the different mouse models	1538:1563	the different mouse models of allergic asthma created by the sensitization with different adjuvants	1538:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	9	44	theme	T-bet+	1289:1294	arg1	ILCs					1296:1299	T-bet+ ILCs	1289:1299	T-bet+ ILCs	1289:1299	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	10	45	theme	different	1618:1626	arg1	adjuvants					1628:1636	different adjuvants	1618:1636	different adjuvants	1618:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	1	46	theme	type	183:186	arg1	immunity					190:197	type 2 immunity	183:197	type 2 immunity	183:197	BACKGROUND Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity.
32563228	4	47	theme	asthma	517:522	arg1	models					524:529	mouse asthma models	511:529	mouse asthma models induced by ovalbumin (OVA)	511:556	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	8	48	theme	elevated	1141:1148	arg1	levels					1150:1155	the most elevated levels	1132:1155	the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells	1132:1220	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	7	49	theme	eosinophil	979:988	arg1	levels					990:995	the highest eosinophil levels	967:995	the highest eosinophil levels	967:995	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	7	50	located	detected	1070:1077	arg1	group					1090:1094	the LPS group	1082:1094	the LPS group	1082:1094	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	7	50	located	detected	1070:1077	arg2	levels					1058:1063	the highest neutrophil levels	1035:1063	the highest neutrophil levels	1035:1063	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	2	51	theme	phenotypes	290:299	arg1	phenotypes					290:299	phenotypes	290:299	phenotypes	290:299	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	2	51	theme	phenotypes	290:299	arg1	variety					279:285	a variety	277:285	a variety of phenotypes and endotypes	277:313	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	2	51	theme	phenotypes	290:299	arg1	endotypes					305:313	endotypes	305:313	endotypes	305:313	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	8	52	dep	lung	1229:1232	arg1	the					1225:1227	the	1225:1227	the	1225:1227	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	10	53	theme	immune	1463:1468	arg1	cells					1470:1474	immune cells	1463:1474	immune cells	1463:1474	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	9	54	theme	papain	1320:1325	arg1	group					1327:1331	the papain group	1316:1331	the papain group	1316:1331	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	8	55	theme	RORγt+	1165:1170	arg1	ILCs					1195:1198	ILCs	1195:1198	ILCs	1195:1198	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	8	55	theme	RORγt+	1165:1170	arg1	cells					1188:1192	RORγt+ innate lymphoid cells	1165:1192	RORγt+ innate lymphoid cells (ILCs)	1165:1199	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	6	56	from	cells	805:809	arg1	fluid					843:847	bronchoalveolar lavage (BAL) fluid	814:847	bronchoalveolar lavage (BAL) fluid	814:847	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	6	56	from	cells	805:809	arg1	mLN					884:886	mLN	884:886	mLN	884:886	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	6	56	from	cells	805:809	arg1	node					878:881	mediastinal lymph node	860:881	mediastinal lymph node (mLN)	860:887	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	6	56	from	cells	805:809	arg1	lung					850:853	lung	850:853	lung	850:853	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	5	57	theme	various	663:669	arg1	CpG					745:747	CpG	745:747	CpG	745:747	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	5	57	theme	various	663:669	arg1	lipopolysaccharide					716:733	lipopolysaccharide	716:733	lipopolysaccharide (LPS)	716:739	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	5	57	theme	various	663:669	arg1	adjuvants					671:679	various adjuvants	663:679	various adjuvants	663:679	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	5	57	theme	various	663:669	arg1	Alhydrogel					689:698	Alhydrogel	689:698	Alhydrogel (alum)	689:705	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	5	57	theme	various	663:669	arg1	papain					708:713	papain	708:713	papain	708:713	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	7	58	theme	lung	947:950	arg1	fluid					960:964	the lung and BAL fluid	943:964	fluid	960:964	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	8	59	theme	lymphoid	1179:1186	arg1	ILCs					1195:1198	ILCs	1195:1198	ILCs	1195:1198	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	8	59	theme	lymphoid	1179:1186	arg1	cells					1188:1192	RORγt+ innate lymphoid cells	1165:1192	RORγt+ innate lymphoid cells (ILCs)	1165:1199	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	2	60	theme	complex	250:256	arg1	disease					258:264	a complex disease	248:264	a complex disease displaying a variety of phenotypes and endotypes	248:313	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	2	60	theme	complex	250:256	arg1	asthma					238:243	asthma	238:243	asthma	238:243	However, recent studies revealed that asthma is a complex disease displaying a variety of phenotypes and endotypes.
32563228	7	61	located	observed	1002:1009	arg2	levels					990:995	the highest eosinophil levels	967:995	the highest eosinophil levels	967:995	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	7	61	located	observed	1002:1009	arg1	group					1023:1027	the alum group	1014:1027	the alum group	1014:1027	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	7	61	located	observed	1002:1009	arg1	fluid					960:964	the lung and BAL fluid	943:964	fluid	960:964	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	9	62	theme	IFN-γ+	1355:1360	arg1	cells					1365:1369	IFN-γ+ Th cells	1355:1369	IFN-γ+ Th cells	1355:1369	In the lung, the number of T-bet+ ILCs was highest in the papain group whereas the number of IFN-γ+ Th cells was highest in the CpG group.
32563228	0	63	theme	airway	71:76	arg1	inflammation					78:89	allergic airway inflammation	62:89	allergic airway inflammation	62:89	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	1	64	theme	inflammatory	150:161	arg1	asthma					112:117	BACKGROUND Allergic asthma	92:117	BACKGROUND Allergic asthma	92:117	BACKGROUND Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity.
32563228	1	64	theme	inflammatory	150:161	arg1	disease					163:169	an inflammatory disease	147:169	an inflammatory disease mediated by type 2 immunity	147:197	BACKGROUND Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity.
32563228	10	65	with	sensitization	1599:1611	arg1	adjuvants					1628:1636	different adjuvants	1618:1636	different adjuvants	1618:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	0	66	from	effects	9:15	arg1	model					53:57	the mouse model	43:57	the mouse model of allergic airway inflammation	43:89	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	8	67	theme	IL-17A+	1205:1211	arg1	cells					1216:1220	IL-17A+ Th cells	1205:1220	IL-17A+ Th cells	1205:1220	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	5	68	theme	METHODS	586:592	arg1	Mice					594:597	METHODS Mice	586:597	METHODS Mice	586:597	METHODS Mice were sensitized intraperitoneally with the admixture of OVA and various adjuvants such as Alhydrogel (alum), papain, lipopolysaccharide (LPS), or CpG, and subsequently challenged with OVA intranasally.
32563228	6	69	theme	cytometric	911:920	arg1	analyses					922:929	flow cytometric analyses	906:929	flow cytometric analyses	906:929	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	8	70	theme	cells	1216:1220	arg1	levels					1150:1155	the most elevated levels	1132:1155	the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells	1132:1220	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	7	71	dep	RESULTS	932:938	arg1	observed					1002:1009	observed	1002:1009	were observed in the alum group while the highest neutrophil levels were detected in the LPS group	997:1094	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	4	72	from	variety	564:570	arg1	models					524:529	mouse asthma models	511:529	mouse asthma models induced by ovalbumin (OVA)	511:556	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	4	73	theme	cellular	483:490	arg1	characteristics					492:506	immunologic cellular characteristics	471:506	immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA)	471:556	The aim of our study was to determine immunologic cellular characteristics in mouse asthma models induced by ovalbumin (OVA) and a variety of adjuvants.
32563228	10	74	theme	expression	1480:1489	arg1	composition					1448:1458	the composition	1444:1458	the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1444:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	3	75	theme	allergic	380:387	arg1	asthma					389:394	allergic asthma	380:394	allergic asthma sensitized with different adjuvants	380:430	OBJECTIVE We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants.
32563228	10	76	theme	cells	1470:1474	arg1	composition					1448:1458	the composition	1444:1458	the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1444:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	0	77	theme	different	20:28	arg1	adjuvants					30:38	different adjuvants	20:38	different adjuvants	20:38	Distinct effects of different adjuvants in the mouse model of allergic airway inflammation.
32563228	1	78	theme	BACKGROUND	92:101	arg1	asthma					112:117	BACKGROUND Allergic asthma	92:117	BACKGROUND Allergic asthma	92:117	BACKGROUND Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity.
32563228	1	78	theme	BACKGROUND	92:101	arg1	disease					163:169	an inflammatory disease	147:169	an inflammatory disease mediated by type 2 immunity	147:197	BACKGROUND Allergic asthma was typically considered as an inflammatory disease mediated by type 2 immunity.
32563228	3	79	dep	OBJECTIVE	316:324	arg1	examined					329:336	examined	329:336	examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants	329:430	OBJECTIVE We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants.
32563228	8	80	theme	lymph	1250:1254	arg1	node					1256:1259	mediastinal lymph node	1238:1259	mediastinal lymph node	1238:1259	Meanwhile, the LPS group exhibited the most elevated levels of both RORγt+ innate lymphoid cells (ILCs) and IL-17A+ Th cells in the lung and mediastinal lymph node.
32563228	6	81	theme	lavage	830:835	arg1	fluid					843:847	bronchoalveolar lavage (BAL) fluid	814:847	bronchoalveolar lavage (BAL) fluid	814:847	The cells in bronchoalveolar lavage (BAL) fluid, lung, and mediastinal lymph node (mLN) were examined by flow cytometric analyses.
32563228	3	82	theme	different	412:420	arg1	adjuvants					422:430	different adjuvants	412:430	different adjuvants	412:430	OBJECTIVE We examined cellular phenotypes in the mouse model of allergic asthma sensitized with different adjuvants.
32563228	10	83	theme	cytokines	1494:1502	arg1	expression					1480:1489	expression	1480:1489	expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants	1480:1636	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	10	83	theme	cytokines	1494:1502	arg1	cells					1470:1474	immune cells	1463:1474	immune cells	1463:1474	CONCLUSIONS Notable variances are found in the composition of immune cells and expression of cytokines at the site of pathogenesis among the different mouse models of allergic asthma created by the sensitization with different adjuvants.
32563228	7	84	theme	highest	1039:1045	arg1	levels					1058:1063	the highest neutrophil levels	1035:1063	the highest neutrophil levels	1035:1063	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
32563228	7	85	theme	highest	971:977	arg1	levels					990:995	the highest eosinophil levels	967:995	the highest eosinophil levels	967:995	RESULTS In the lung and BAL fluid, the highest eosinophil levels were observed in the alum group while the highest neutrophil levels were detected in the LPS group.
36151503	1	0	dep	composition	297:307	arg1	the					278:280	the	278:280	the	278:280	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	1	theme	azido	158:162	arg1	analog					164:169	the exogenous azido analog	144:169	the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3	144:224	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	4	2	dep	Escherichia	668:678	arg1	coli					680:683	coli	680:683	coli	680:683	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	1	3	theme	molecular	256:264	arg1	analysis					266:273	both live-cell and molecular analysis	237:273	both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria	237:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	4	4	theme	NanT	754:757	arg1	capable					772:778	capable	772:778	capable	772:778	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	4	theme	NanT	754:757	arg1	transporter					742:752	the sialic acid transporter NanT	726:757	the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS	726:878	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	1	5	from	biosynthesis	313:324	arg1	bacteria					353:360	different Gram-negative bacteria	329:360	different Gram-negative bacteria	329:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	4	6	theme	sialic	730:735	arg1	capable					772:778	capable	772:778	capable	772:778	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	6	theme	sialic	730:735	arg1	transporter					742:752	the sialic acid transporter NanT	726:757	the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS	726:878	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	7	theme	Escherichia	668:678	arg1	bacteria					659:666	the Gram-negative bacteria	641:666	the Gram-negative bacteria	641:666	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	8	contain	possess	718:724	arg2	transporter					742:752	the sialic acid transporter NanT	726:757	the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS	726:878	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	8	contain	possess	718:724	arg2	capable					772:778	capable	772:778	capable	772:778	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	8	contain	possess	718:724	arg1	bacteria					659:666	the Gram-negative bacteria	641:666	the Gram-negative bacteria	641:666	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	1	9	from	composition	297:307	arg1	bacteria					353:360	different Gram-negative bacteria	329:360	different Gram-negative bacteria	329:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	4	10	dep	Klebsiella	689:698	arg1	pneumoniae					700:709	pneumoniae	700:709	pneumoniae	700:709	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	1	11	theme	3-deoxy-D-manno-oct-2-ulosonic	174:203	arg1	acid					205:208	3-deoxy-D-manno-oct-2-ulosonic acid	174:208	3-deoxy-D-manno-oct-2-ulosonic acid (Kdo)	174:214	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	11	theme	3-deoxy-D-manno-oct-2-ulosonic	174:203	arg1	Kdo					211:213	Kdo	211:213	Kdo	211:213	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	2	12	theme	fluorescent	458:468	arg1	dye					470:472	a fluorescent dye	456:472	a fluorescent dye	456:472	Here, we describe Kdo-N3 incorporation into bacterial cells, followed by click labeling with a fluorescent dye.
36151503	4	13	theme	nascent	868:874	arg1	LPS					876:878	nascent LPS	868:878	nascent LPS	868:878	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	1	14	theme	Metabolic	91:99	arg1	labeling					101:108	Metabolic labeling	91:108	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3	91:224	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	15	theme	acid	205:208	arg1	analog					164:169	the exogenous azido analog	144:169	the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3	144:224	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	0	16	theme	Metabolic	0:8	arg1	Incorporation					10:22	Metabolic Incorporation	0:22	Metabolic Incorporation of Azido-Sugars into LPS	0:47	Metabolic Incorporation of Azido-Sugars into LPS to Enable Live-Cell Fluorescence Imaging.
36151503	0	17	theme	Azido-Sugars	27:38	arg1	Incorporation					10:22	Metabolic Incorporation	0:22	Metabolic Incorporation of Azido-Sugars into LPS	0:47	Metabolic Incorporation of Azido-Sugars into LPS to Enable Live-Cell Fluorescence Imaging.
36151503	1	18	theme	outer	282:286	arg1	composition					297:307	outer membrane composition	282:307	outer membrane composition	282:307	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	19	from	analysis	266:273	arg1	bacteria					353:360	different Gram-negative bacteria	329:360	different Gram-negative bacteria	329:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	3	20	theme	intact	559:564	arg1	cells					566:570	intact cells	559:570	intact cells	559:570	The fluorescently labeled LPS can be analyzed from lysed cells by SDS-PAGE and from intact cells by microscopy and flow cytometry.
36151503	2	21	theme	click	436:440	arg1	labeling					442:449	click labeling	436:449	click labeling with a fluorescent dye	436:472	Here, we describe Kdo-N3 incorporation into bacterial cells, followed by click labeling with a fluorescent dye.
36151503	1	22	with	labeling	101:108	arg1	analog					164:169	the exogenous azido analog	144:169	the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3	144:224	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	23	theme	membrane	288:295	arg1	composition					297:307	outer membrane composition	282:307	outer membrane composition	282:307	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	24	theme	lipopolysaccharides	113:131	arg1	labeling					101:108	Metabolic labeling	91:108	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3	91:224	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	3	25	theme	lysed	526:530	arg1	cells					532:536	lysed cells	526:536	lysed cells	526:536	The fluorescently labeled LPS can be analyzed from lysed cells by SDS-PAGE and from intact cells by microscopy and flow cytometry.
36151503	1	26	theme	composition	297:307	arg1	analysis					266:273	both live-cell and molecular analysis	237:273	both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria	237:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	4	27	theme	Gram-negative	645:657	arg1	bacteria					659:666	the Gram-negative bacteria	641:666	the Gram-negative bacteria	641:666	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	1	28	theme	Kdo-N3	219:224	arg1	analog					164:169	the exogenous azido analog	144:169	the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3	144:224	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	29	theme	biosynthesis	313:324	arg1	analysis					266:273	both live-cell and molecular analysis	237:273	both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria	237:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	2	30	with	labeling	442:449	arg1	dye					470:472	a fluorescent dye	456:472	a fluorescent dye	456:472	Here, we describe Kdo-N3 incorporation into bacterial cells, followed by click labeling with a fluorescent dye.
36151503	1	31	from	bacteria	353:360	arg1	analysis					266:273	both live-cell and molecular analysis	237:273	both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria	237:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	3	32	theme	flow	590:593	arg1	cytometry					595:603	flow cytometry	590:603	flow cytometry	590:603	The fluorescently labeled LPS can be analyzed from lysed cells by SDS-PAGE and from intact cells by microscopy and flow cytometry.
36151503	1	33	theme	different	329:337	arg1	bacteria					353:360	different Gram-negative bacteria	329:360	different Gram-negative bacteria	329:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	4	34	theme	Kdo	806:808	arg1	analogs					818:824	exogenous Kdo and Kdo analogs	796:824	exogenous Kdo and Kdo analogs	796:824	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	3	35	theme	labeled	493:499	arg1	LPS					501:503	The fluorescently labeled LPS	475:503	The fluorescently labeled LPS	475:503	The fluorescently labeled LPS can be analyzed from lysed cells by SDS-PAGE and from intact cells by microscopy and flow cytometry.
36151503	4	36	theme	exogenous	796:804	arg1	analogs					818:824	exogenous Kdo and Kdo analogs	796:824	exogenous Kdo and Kdo analogs	796:824	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	37	theme	Klebsiella	689:698	arg1	bacteria					659:666	the Gram-negative bacteria	641:666	the Gram-negative bacteria	641:666	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	38	theme	acid	737:740	arg1	capable					772:778	capable	772:778	capable	772:778	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	4	38	theme	acid	737:740	arg1	transporter					742:752	the sialic acid transporter NanT	726:757	the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS	726:878	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	0	39	theme	Fluorescence	69:80	arg1	Imaging					82:88	Live-Cell Fluorescence Imaging	59:88	Live-Cell Fluorescence Imaging	59:88	Metabolic Incorporation of Azido-Sugars into LPS to Enable Live-Cell Fluorescence Imaging.
36151503	4	40	theme	Kdo	814:816	arg1	analogs					818:824	exogenous Kdo and Kdo analogs	796:824	exogenous Kdo and Kdo analogs	796:824	These methods have been applied to the Gram-negative bacteria Escherichia coli and Klebsiella pneumoniae, which possess the sialic acid transporter NanT that is also capable of transporting exogenous Kdo and Kdo analogs into the cytoplasm for incorporation into nascent LPS.
36151503	2	41	theme	Kdo-N3	381:386	arg1	incorporation					388:400	Kdo-N3 incorporation	381:400	Kdo-N3 incorporation into bacterial cells	381:421	Here, we describe Kdo-N3 incorporation into bacterial cells, followed by click labeling with a fluorescent dye.
36151503	2	42	theme	bacterial	407:415	arg1	cells					417:421	bacterial cells	407:421	bacterial cells	407:421	Here, we describe Kdo-N3 incorporation into bacterial cells, followed by click labeling with a fluorescent dye.
36151503	0	43	theme	Live-Cell	59:67	arg1	Imaging					82:88	Live-Cell Fluorescence Imaging	59:88	Live-Cell Fluorescence Imaging	59:88	Metabolic Incorporation of Azido-Sugars into LPS to Enable Live-Cell Fluorescence Imaging.
36151503	1	44	theme	Gram-negative	339:351	arg1	bacteria					353:360	different Gram-negative bacteria	329:360	different Gram-negative bacteria	329:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	45	theme	exogenous	148:156	arg1	analog					164:169	the exogenous azido analog	144:169	the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3	144:224	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
36151503	1	46	theme	live-cell	242:250	arg1	analysis					266:273	both live-cell and molecular analysis	237:273	both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria	237:360	Metabolic labeling of lipopolysaccharides (LPS) with the exogenous azido analog of 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) or Kdo-N3 allows for both live-cell and molecular analysis of the outer membrane composition and biosynthesis in different Gram-negative bacteria.
35944283	9	0	theme	productivity	1597:1608	arg1	preservation					1560:1571	preservation	1560:1571	preservation of soil health and crop productivity	1560:1608	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	1	1	theme	community	157:165	arg1	composition					167:177	soil bacterial community composition	142:177	soil bacterial community composition	142:177	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	4	2	theme	studied	559:565	arg1	plots					567:571	the studied plots	555:571	the studied plots	555:571	Soil samples collected from the studied plots were used to quantify Escherichia coli by plate counts, and Clostridium perfringens by qPCR.
35944283	0	3	theme	community	76:84	arg1	structure					86:94	soil bacterial community structure	61:94	soil bacterial community structure	61:94	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	2	4	from	plots	426:430	arg1	Valley					446:451	Valley	446:451	Valley	446:451	Litter (27.5 T/ha) was applied to 24 agricultural plots in the Fraser Valley of British Columbia.
35944283	9	5	theme	anaerobic	1491:1499	arg1	digestion					1501:1509	anaerobic digestion	1491:1509	anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity	1491:1608	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	1	6	theme	salinomycin	348:358	arg1	mix					341:343	mix	341:343	mix	341:343	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	6	theme	salinomycin	348:358	arg1	bacitracin					313:322	bacitracin	313:322	bacitracin	313:322	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	6	theme	salinomycin	348:358	arg1	penicillin					301:310	penicillin	301:310	penicillin	301:310	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	6	theme	salinomycin	348:358	arg1	bambermycin					288:298	bambermycin	288:298	bambermycin	288:298	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	6	theme	salinomycin	348:358	arg1	salinomycin					325:335	salinomycin	325:335	salinomycin	325:335	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	7	7	theme	relative	1048:1055	arg1	abundance					1057:1065	the relative abundance	1044:1065	the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil	1044:1123	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	6	8	theme	population	780:789	arg1	CFU/g					819:823	5.4 log CFU/g	811:823	5.4 log CFU/g	811:823	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	6	8	theme	population	780:789	arg1	size					791:794	the population size	776:794	the population size of E. coli	776:805	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	8	9	theme	fertilized	1274:1283	arg1	ones					1285:1288	the fertilized ones	1270:1288	the fertilized ones	1270:1288	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	7	10	from	birds	1017:1021	arg1	Fertilization					967:979	Fertilization	967:979	Fertilization with litter from antibiotic-treated birds	967:1021	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	0	11	from	chickens	49:56	arg1	litter					11:16	litter	11:16	litter from antimicrobial-fed broiler chickens	11:56	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	0	11	from	chickens	49:56	arg1	Effects					0:6	Effects	0:6	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity	0:108	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	7	12	theme	Firmicutes	1106:1115	arg1	abundance					1057:1065	the relative abundance	1044:1065	the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil	1044:1123	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	8	13	dep	higher	1218:1223	arg1	P < 0.05					1226:1233	P < 0.05	1226:1233	P < 0.05	1226:1233	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	8	14	theme	alpha	1186:1190	arg1	higher					1218:1223	higher	1218:1223	higher	1218:1223	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	8	14	theme	alpha	1186:1190	arg1	parameters					1202:1211	The alpha diversity parameters	1182:1211	The alpha diversity parameters	1182:1211	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	5	15	theme	microbiota	713:722	arg1	analysis					724:731	microbiota analysis	713:731	microbiota analysis	713:731	The 16S rRNA gene sequencing was performed for microbiota analysis.
35944283	1	16	theme	bacitracin	364:373	arg1	mix					341:343	mix	341:343	mix	341:343	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	16	theme	bacitracin	364:373	arg1	bacitracin					313:322	bacitracin	313:322	bacitracin	313:322	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	16	theme	bacitracin	364:373	arg1	penicillin					301:310	penicillin	301:310	penicillin	301:310	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	16	theme	bacitracin	364:373	arg1	bambermycin					288:298	bambermycin	288:298	bambermycin	288:298	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	16	theme	bacitracin	364:373	arg1	salinomycin					325:335	salinomycin	325:335	salinomycin	325:335	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	7	17	theme	Actinobacteria	1086:1099	arg1	abundance					1057:1065	the relative abundance	1044:1065	the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil	1044:1123	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	1	18	from	changes	131:137	arg1	response					196:203	response	196:203	response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin	196:373	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	18	from	changes	131:137	arg1	diversity					183:191	diversity	183:191	diversity	183:191	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	18	from	changes	131:137	arg1	composition					167:177	soil bacterial community composition	142:177	soil bacterial community composition	142:177	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	3	19	theme	negative	509:516	arg1	control					518:524	a negative control	507:524	a negative control	507:524	Nonfertilized plots were used as a negative control.
35944283	3	19	theme	negative	509:516	arg1	plots					488:492	Nonfertilized plots	474:492	Nonfertilized plots	474:492	Nonfertilized plots were used as a negative control.
35944283	7	20	theme	Verrucomicrobia	1158:1172	arg1	groups					1174:1179	Verrucomicrobia groups	1158:1179	Verrucomicrobia groups	1158:1179	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	9	21	theme	poultry	1514:1520	arg1	litter					1522:1527	poultry litter	1514:1527	poultry litter before application to land for preservation of soil health and crop productivity	1514:1608	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	9	22	theme	litter	1522:1527	arg1	autoclaving					1475:1485	autoclaving	1475:1485	autoclaving	1475:1485	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	9	22	theme	litter	1522:1527	arg1	composting					1463:1472	composting	1463:1472	composting	1463:1472	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	9	22	theme	litter	1522:1527	arg1	digestion					1501:1509	anaerobic digestion	1491:1509	anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity	1491:1608	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	7	23	with	Fertilization	967:979	arg1	litter					986:991	litter	986:991	litter	986:991	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	5	24	theme	gene	679:682	arg1	sequencing					684:693	The 16S rRNA gene sequencing	666:693	The 16S rRNA gene sequencing	666:693	The 16S rRNA gene sequencing was performed for microbiota analysis.
35944283	6	25	theme	coli	910:913	arg1	CFU/g					898:902	5.2 and 1.4 log CFU/g	882:902	5.2 and 1.4 log CFU/g of E. coli	882:913	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	8	26	theme	litter	1307:1312	arg1	application					1314:1324	litter application	1307:1324	litter application	1307:1324	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	8	26	theme	litter	1307:1312	arg1	factor					1338:1343	a major factor	1330:1343	a major factor	1330:1343	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	4	27	theme	Clostridium	633:643	arg1	perfringens					645:655	Clostridium perfringens	633:655	Clostridium perfringens	633:655	Soil samples collected from the studied plots were used to quantify Escherichia coli by plate counts, and Clostridium perfringens by qPCR.
35944283	2	28	theme	agricultural	413:424	arg1	plots					426:430	24 agricultural plots	410:430	24 agricultural plots in the Fraser Valley of British Columbia	410:471	Litter (27.5 T/ha) was applied to 24 agricultural plots in the Fraser Valley of British Columbia.
35944283	4	29	theme	Soil	527:530	arg1	samples					532:538	Soil samples	527:538	Soil samples collected from the studied plots	527:571	Soil samples collected from the studied plots were used to quantify Escherichia coli by plate counts, and Clostridium perfringens by qPCR.
35944283	5	30	theme	16S	670:672	arg1	sequencing					684:693	The 16S rRNA gene sequencing	666:693	The 16S rRNA gene sequencing	666:693	The 16S rRNA gene sequencing was performed for microbiota analysis.
35944283	0	31	theme	litter	11:16	arg1	Effects					0:6	Effects	0:6	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity	0:108	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	5	32	theme	rRNA	674:677	arg1	sequencing					684:693	The 16S rRNA gene sequencing	666:693	The 16S rRNA gene sequencing	666:693	The 16S rRNA gene sequencing was performed for microbiota analysis.
35944283	6	33	from	application	751:761	arg1	December					766:773	December	766:773	December	766:773	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	0	34	theme	broiler	41:47	arg1	chickens					49:56	antimicrobial-fed broiler chickens	23:56	antimicrobial-fed broiler chickens	23:56	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	4	35	used	used	578:581	arg2	samples					532:538	Soil samples	527:538	Soil samples collected from the studied plots	527:571	Soil samples collected from the studied plots were used to quantify Escherichia coli by plate counts, and Clostridium perfringens by qPCR.
35944283	8	36	theme	diversity	1192:1200	arg1	higher					1218:1223	higher	1218:1223	higher	1218:1223	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	8	36	theme	diversity	1192:1200	arg1	parameters					1202:1211	The alpha diversity parameters	1182:1211	The alpha diversity parameters	1182:1211	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	9	37	theme	efficient	1419:1427	arg1	strategies					1447:1456	efficient litter management strategies	1419:1456	efficient litter management strategies	1419:1456	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	0	38	theme	antimicrobial-fed	23:39	arg1	chickens					49:56	antimicrobial-fed broiler chickens	23:56	antimicrobial-fed broiler chickens	23:56	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	6	39	theme	log	894:896	arg1	CFU/g					898:902	5.2 and 1.4 log CFU/g	882:902	5.2 and 1.4 log CFU/g of E. coli	882:913	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	1	40	with	fertilization	208:220	arg1	litter					227:232	litter	227:232	litter	227:232	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	8	41	theme	major	1332:1336	arg1	application					1314:1324	litter application	1307:1324	litter application	1307:1324	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	8	41	theme	major	1332:1336	arg1	factor					1338:1343	a major factor	1330:1343	a major factor	1330:1343	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	6	42	theme	litter	744:749	arg1	application					751:761	litter application	744:761	litter application in December	744:773	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	4	43	theme	plate	615:619	arg1	counts					621:626	plate counts	615:626	plate counts	615:626	Soil samples collected from the studied plots were used to quantify Escherichia coli by plate counts, and Clostridium perfringens by qPCR.
35944283	7	44	theme	Proteobacteria	1070:1083	arg1	abundance					1057:1065	the relative abundance	1044:1065	the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil	1044:1123	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	6	45	theme	log	815:817	arg1	CFU/g					819:823	5.4 log CFU/g	811:823	5.4 log CFU/g	811:823	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	6	45	theme	log	815:817	arg1	size					791:794	the population size	776:794	the population size of E. coli	776:805	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	9	46	theme	management	1436:1445	arg1	strategies					1447:1456	efficient litter management strategies	1419:1456	efficient litter management strategies	1419:1456	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	0	47	from	Effects	0:6	arg1	diversity					100:108	diversity	100:108	diversity	100:108	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	0	47	from	Effects	0:6	arg1	structure					86:94	soil bacterial community structure	61:94	soil bacterial community structure	61:94	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	0	47	from	Effects	0:6	arg1	chickens					49:56	antimicrobial-fed broiler chickens	23:56	antimicrobial-fed broiler chickens	23:56	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	7	48	from	abundance	1057:1065	arg1	soil					1120:1123	soil	1120:1123	soil	1120:1123	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	7	49	theme	antibiotic-treated	998:1015	arg1	birds					1017:1021	antibiotic-treated birds	998:1021	antibiotic-treated birds	998:1021	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	1	50	with	diet	254:257	arg1	mix					341:343	mix	341:343	mix	341:343	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	50	with	diet	254:257	arg1	bacitracin					313:322	bacitracin	313:322	bacitracin	313:322	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	50	with	diet	254:257	arg1	penicillin					301:310	penicillin	301:310	penicillin	301:310	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	50	with	diet	254:257	arg1	bambermycin					288:298	bambermycin	288:298	bambermycin	288:298	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	1	50	with	diet	254:257	arg1	salinomycin					325:335	salinomycin	325:335	salinomycin	325:335	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	3	51	theme	Nonfertilized	474:486	arg1	control					518:524	a negative control	507:524	a negative control	507:524	Nonfertilized plots were used as a negative control.
35944283	3	51	theme	Nonfertilized	474:486	arg1	plots					488:492	Nonfertilized plots	474:492	Nonfertilized plots	474:492	Nonfertilized plots were used as a negative control.
35944283	8	52	theme	bacterial	1365:1373	arg1	communities					1375:1385	the soil bacterial communities	1356:1385	the soil bacterial communities	1356:1385	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	9	53	theme	soil	1576:1579	arg1	health					1581:1586	soil health	1576:1586	soil health	1576:1586	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	8	54	theme	soil	1360:1363	arg1	communities					1375:1385	the soil bacterial communities	1356:1385	the soil bacterial communities	1356:1385	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	9	55	theme	crop	1592:1595	arg1	productivity					1597:1608	crop productivity	1592:1608	crop productivity	1592:1608	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	1	56	from	chickens	239:246	arg1	fertilization					208:220	fertilization	208:220	fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin	208:373	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	6	57	theme	coli	802:805	arg1	CFU/g					819:823	5.4 log CFU/g	811:823	5.4 log CFU/g	811:823	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	6	57	theme	coli	802:805	arg1	size					791:794	the population size	776:794	the population size of E. coli	776:805	Following litter application in December, the population size of E. coli was 5.4 log CFU/g; however, regardless of treatments, the results revealed 5.2 and 1.4 log CFU/g of E. coli in soil sampled in January and March, respectively.
35944283	9	58	theme	health	1581:1586	arg1	preservation					1560:1571	preservation	1560:1571	preservation of soil health and crop productivity	1560:1608	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	8	59	theme	nonfertilized	1239:1251	arg1	soil					1253:1256	nonfertilized soil	1239:1256	nonfertilized soil compared to the fertilized ones	1239:1288	The alpha diversity parameters were higher (P < 0.05) in nonfertilized soil compared to the fertilized ones, suggesting that litter application was a major factor in shaping the soil bacterial communities.
35944283	0	60	theme	bacterial	66:74	arg1	structure					86:94	soil bacterial community structure	61:94	soil bacterial community structure	61:94	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	9	61	theme	litter	1429:1434	arg1	strategies					1447:1456	efficient litter management strategies	1419:1456	efficient litter management strategies	1419:1456	These results may help develop efficient litter management strategies like composting, autoclaving, or anaerobic digestion of poultry litter before application to land for preservation of soil health and crop productivity.
35944283	7	62	dep	increased	1023:1031	arg1	P < 0.05					1034:1041	P < 0.05	1034:1041	P < 0.05	1034:1041	Fertilization with litter from antibiotic-treated birds increased (P < 0.05) the relative abundance of Proteobacteria, Actinobacteria, and Firmicutes in soil, but decreased Acidobacteria and Verrucomicrobia groups.
35944283	1	63	theme	soil	142:145	arg1	composition					167:177	soil bacterial community composition	142:177	soil bacterial community composition	142:177	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35944283	0	64	theme	soil	61:64	arg1	structure					86:94	soil bacterial community structure	61:94	soil bacterial community structure	61:94	Effects of litter from antimicrobial-fed broiler chickens on soil bacterial community structure and diversity.
35944283	3	65	used	used	499:502	arg2	plots					488:492	Nonfertilized plots	474:492	Nonfertilized plots	474:492	Nonfertilized plots were used as a negative control.
35944283	3	65	used	used	499:502	arg2	control					518:524	a negative control	507:524	a negative control	507:524	Nonfertilized plots were used as a negative control.
35944283	1	66	theme	bacterial	147:155	arg1	composition					167:177	soil bacterial community composition	142:177	soil bacterial community composition	142:177	This study examined changes in soil bacterial community composition and diversity in response to fertilization with litter from chickens fed a diet without antibiotics and with bambermycin, penicillin, bacitracin, salinomycin, or mix of salinomycin and bacitracin.
35655785	0	0	theme	Microbial	85:93	arg1	Metabolism					106:115	Microbial Tryptophan Metabolism	85:115	Microbial Tryptophan Metabolism	85:115	Ginsenoside Rg1 Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Microbial Tryptophan Metabolism.
35655785	4	1	theme	colon	615:619	arg1	pathology					628:636	colon tissue pathology	615:636	colon tissue pathology	615:636	Body weight, colon length, colon tissue pathology, and colon tissue inflammatory cytokines were assessed.
35655785	5	2	theme	composition	698:708	arg1	structure					710:718	The composition structure	694:718	The composition structure of gut microbiota	694:736	The composition structure of gut microbiota was profiled using 16s rRNA sequencing.
35655785	7	3	theme	colonic	983:989	arg1	inflammation					991:1002	colonic inflammation	983:1002	colonic inflammation	983:1002	The results showed that Rg1 significantly ameliorated DSS-induced colonic injury and colonic inflammation.
35655785	1	4	theme	chronic	147:153	arg1	disorder					182:189	a chronic and recurrent inflammatory disorder	145:189	a chronic and recurrent inflammatory disorder in the gastrointestinal tract	145:219	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	1	4	theme	chronic	147:153	arg1	colitis					129:135	Ulcerative colitis	118:135	Ulcerative colitis (UC)	118:140	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	2	5	with	compound	298:305	arg1	bioavailability					316:330	low bioavailability	312:330	low bioavailability	312:330	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	9	6	theme	vitamin	1234:1240	arg1	metabolism					1245:1254	vitamin B6 metabolism	1234:1254	vitamin B6 metabolism	1234:1254	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	7	theme	B6	1242:1243	arg1	metabolism					1245:1254	vitamin B6 metabolism	1234:1254	vitamin B6 metabolism	1234:1254	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	10	8	theme	tryptophan	1355:1364	arg1	indole-3-carboxaldehyde					1402:1424	indole-3-carboxaldehyde	1402:1424	indole-3-carboxaldehyde	1402:1424	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	8	theme	tryptophan	1355:1364	arg1	acid					1443:1446	indole-3-lactic acid	1427:1446	indole-3-lactic acid	1427:1446	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	8	theme	tryptophan	1355:1364	arg1	acid					1467:1470	3-indolepropionic acid	1449:1470	3-indolepropionic acid	1449:1470	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	8	theme	tryptophan	1355:1364	arg1	niacinamide					1477:1487	niacinamide	1477:1487	niacinamide	1477:1487	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	8	theme	tryptophan	1355:1364	arg1	metabolites					1366:1376	tryptophan metabolites	1355:1376	tryptophan metabolites	1355:1376	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	2	9	theme	acute	340:344	arg1	colitis					359:365	the acute experimental colitis	336:365	the acute experimental colitis mice induced by dextran sulfate sodium (DSS)	336:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	11	10	theme	microbiota	1741:1750	arg1	composition					1752:1762	gut microbiota composition	1737:1762	gut microbiota composition	1737:1762	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	11	11	theme	intestinal	1604:1613	arg1	barrier					1615:1621	the intestinal barrier	1600:1621	the intestinal barrier	1600:1621	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	9	12	theme	various	1132:1138	arg1	pathways					1150:1157	various metabolic pathways	1132:1157	various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism	1132:1254	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	12	theme	various	1132:1138	arg1	leucine					1193:1199	leucine	1193:1199	leucine	1193:1199	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	12	theme	various	1132:1138	arg1	valine					1185:1190	valine	1185:1190	valine	1185:1190	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	12	theme	various	1132:1138	arg1	biosynthesis					1217:1228	isoleucine biosynthesis	1206:1228	isoleucine biosynthesis	1206:1228	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	8	13	theme	gut	1060:1062	arg1	composition					1075:1085	gut microbiota composition	1060:1085	gut microbiota composition caused by DSS	1060:1099	In addition, Rg1 also partly reversed the imbalance of gut microbiota composition caused by DSS.
35655785	2	14	theme	natural	290:296	arg1	compound					298:305	a natural compound	288:305	a natural compound with low bioavailability	288:330	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	2	14	theme	natural	290:296	arg1	Rg1					283:285	ginsenoside Rg1	271:285	ginsenoside Rg1	271:285	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	0	15	theme	Tryptophan	95:104	arg1	Metabolism					106:115	Microbial Tryptophan Metabolism	85:115	Microbial Tryptophan Metabolism	85:115	Ginsenoside Rg1 Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Microbial Tryptophan Metabolism.
35655785	11	16	theme	microbial	1768:1776	arg1	metabolism					1789:1798	microbial tryptophan metabolism	1768:1798	microbial tryptophan metabolism	1768:1798	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	9	17	theme	metabolic	1140:1148	arg1	pathways					1150:1157	various metabolic pathways	1132:1157	various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism	1132:1254	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	17	theme	metabolic	1140:1148	arg1	leucine					1193:1199	leucine	1193:1199	leucine	1193:1199	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	17	theme	metabolic	1140:1148	arg1	valine					1185:1190	valine	1185:1190	valine	1185:1190	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	17	theme	metabolic	1140:1148	arg1	biosynthesis					1217:1228	isoleucine biosynthesis	1206:1228	isoleucine biosynthesis	1206:1228	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	2	18	theme	experimental	346:357	arg1	colitis					359:365	the acute experimental colitis	336:365	the acute experimental colitis mice induced by dextran sulfate sodium (DSS)	336:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	8	19	theme	composition	1075:1085	arg1	imbalance					1047:1055	the imbalance	1043:1055	the imbalance of gut microbiota composition caused by DSS	1043:1099	In addition, Rg1 also partly reversed the imbalance of gut microbiota composition caused by DSS.
35655785	4	20	theme	tissue	621:626	arg1	pathology					628:636	colon tissue pathology	615:636	colon tissue pathology	615:636	Body weight, colon length, colon tissue pathology, and colon tissue inflammatory cytokines were assessed.
35655785	10	21	from	levels	1345:1350	arg1	serum					1385:1389	the serum	1381:1389	the serum	1381:1389	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	22	theme	3-indolepropionic	1449:1465	arg1	indole-3-carboxaldehyde					1402:1424	indole-3-carboxaldehyde	1402:1424	indole-3-carboxaldehyde	1402:1424	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	22	theme	3-indolepropionic	1449:1465	arg1	acid					1467:1470	3-indolepropionic acid	1449:1470	3-indolepropionic acid	1449:1470	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	22	theme	3-indolepropionic	1449:1465	arg1	metabolites					1366:1376	tryptophan metabolites	1355:1376	tryptophan metabolites	1355:1376	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	2	23	theme	Rg1	283:285	arg1	effects					260:266	the pharmacological effects	240:266	the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS)	240:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	1	24	theme	recurrent	159:167	arg1	disorder					182:189	a chronic and recurrent inflammatory disorder	145:189	a chronic and recurrent inflammatory disorder in the gastrointestinal tract	145:219	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	1	24	theme	recurrent	159:167	arg1	colitis					129:135	Ulcerative colitis	118:135	Ulcerative colitis (UC)	118:140	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	10	25	theme	metabolites	1530:1540	arg1	levels					1514:1519	the levels	1510:1519	the levels of these metabolites	1510:1540	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	11	26	theme	gut	1737:1739	arg1	composition					1752:1762	gut microbiota composition	1737:1762	gut microbiota composition	1737:1762	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	2	27	theme	ginsenoside	271:281	arg1	compound					298:305	a natural compound	288:305	a natural compound with low bioavailability	288:330	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	2	27	theme	ginsenoside	271:281	arg1	Rg1					283:285	ginsenoside Rg1	271:285	ginsenoside Rg1	271:285	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	1	28	theme	inflammatory	169:180	arg1	disorder					182:189	a chronic and recurrent inflammatory disorder	145:189	a chronic and recurrent inflammatory disorder in the gastrointestinal tract	145:219	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	1	28	theme	inflammatory	169:180	arg1	colitis					129:135	Ulcerative colitis	118:135	Ulcerative colitis (UC)	118:140	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	9	29	theme	prominent	1269:1277	arg1	alteration					1289:1298	the most prominent metabolic alteration	1260:1298	the most prominent metabolic alteration	1260:1298	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	29	theme	prominent	1269:1277	arg1	metabolism					1315:1324	tryptophan metabolism	1304:1324	tryptophan metabolism	1304:1324	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	30	theme	gut	1162:1164	arg1	microbiota					1166:1175	gut microbiota	1162:1175	gut microbiota	1162:1175	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	2	31	theme	low	312:314	arg1	bioavailability					316:330	low bioavailability	312:330	low bioavailability	312:330	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	0	32	theme	Ginsenoside	0:10	arg1	Rg1					12:14	Ginsenoside Rg1	0:14	Ginsenoside Rg1	0:14	Ginsenoside Rg1 Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Microbial Tryptophan Metabolism.
35655785	9	33	theme	metabolic	1279:1287	arg1	alteration					1289:1298	the most prominent metabolic alteration	1260:1298	the most prominent metabolic alteration	1260:1298	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	33	theme	metabolic	1279:1287	arg1	metabolism					1315:1324	tryptophan metabolism	1304:1324	tryptophan metabolism	1304:1324	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	10	34	theme	indole-3-lactic	1427:1441	arg1	indole-3-carboxaldehyde					1402:1424	indole-3-carboxaldehyde	1402:1424	indole-3-carboxaldehyde	1402:1424	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	34	theme	indole-3-lactic	1427:1441	arg1	acid					1443:1446	indole-3-lactic acid	1427:1446	indole-3-lactic acid	1427:1446	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	10	34	theme	indole-3-lactic	1427:1441	arg1	metabolites					1366:1376	tryptophan metabolites	1355:1376	tryptophan metabolites	1355:1376	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	9	35	theme	microbiota	1166:1175	arg1	pathways					1150:1157	various metabolic pathways	1132:1157	various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism	1132:1254	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	35	theme	microbiota	1166:1175	arg1	leucine					1193:1199	leucine	1193:1199	leucine	1193:1199	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	35	theme	microbiota	1166:1175	arg1	valine					1185:1190	valine	1185:1190	valine	1185:1190	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	35	theme	microbiota	1166:1175	arg1	biosynthesis					1217:1228	isoleucine biosynthesis	1206:1228	isoleucine biosynthesis	1206:1228	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	3	36	theme	mg/10	522:526	arg1	Rg1					547:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	3	36	theme	mg/10	522:526	arg1	meanwhile					509:517	meanwhile	509:517	meanwhile	509:517	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	4	37	theme	colon	601:605	arg1	length					607:612	colon length	601:612	colon length	601:612	Body weight, colon length, colon tissue pathology, and colon tissue inflammatory cytokines were assessed.
35655785	0	38	theme	Acute	27:31	arg1	Colitis					44:50	Acute Ulcerative Colitis	27:50	Acute Ulcerative Colitis	27:50	Ginsenoside Rg1 Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Microbial Tryptophan Metabolism.
35655785	2	39	dep	sulfate	391:397	arg1	DSS					407:409	DSS	407:409	DSS	407:409	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	2	39	dep	sulfate	391:397	arg1	sodium					399:404	sodium	399:404	dextran sulfate sodium (DSS)	383:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	10	40	theme	metabolites	1366:1376	arg1	levels					1345:1350	the levels	1341:1350	the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide	1341:1487	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	4	41	theme	inflammatory	656:667	arg1	cytokines					669:677	colon tissue inflammatory cytokines	643:677	colon tissue inflammatory cytokines	643:677	Body weight, colon length, colon tissue pathology, and colon tissue inflammatory cytokines were assessed.
35655785	11	42	theme	composition	1752:1762	arg1	regulation					1723:1732	the regulation	1719:1732	the regulation of gut microbiota composition and microbial tryptophan metabolism	1719:1798	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	2	43	theme	underlying	425:434	arg1	mechanisms					436:445	underlying mechanisms	425:445	underlying mechanisms	425:445	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	6	44	theme	feces	814:818	arg1	profiling					797:805	Global metabolomic profiling	778:805	Global metabolomic profiling of the feces	778:818	Global metabolomic profiling of the feces was performed, and tryptophan and its metabolites in the serum were detected.
35655785	1	45	theme	Ulcerative	118:127	arg1	UC					138:139	UC	138:139	UC	138:139	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	1	45	theme	Ulcerative	118:127	arg1	disorder					182:189	a chronic and recurrent inflammatory disorder	145:189	a chronic and recurrent inflammatory disorder in the gastrointestinal tract	145:219	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	1	45	theme	Ulcerative	118:127	arg1	colitis					129:135	Ulcerative colitis	118:135	Ulcerative colitis (UC)	118:140	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	1	46	theme	gastrointestinal	198:213	arg1	tract					215:219	the gastrointestinal tract	194:219	the gastrointestinal tract	194:219	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	9	47	theme	tryptophan	1304:1313	arg1	alteration					1289:1298	the most prominent metabolic alteration	1260:1298	the most prominent metabolic alteration	1260:1298	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	9	47	theme	tryptophan	1304:1313	arg1	metabolism					1315:1324	tryptophan metabolism	1304:1324	tryptophan metabolism	1304:1324	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	3	48	theme	ginsenoside	535:545	arg1	Rg1					547:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	3	48	theme	ginsenoside	535:545	arg1	meanwhile					509:517	meanwhile	509:517	meanwhile	509:517	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	2	49	from	effects	260:266	arg1	colitis					359:365	the acute experimental colitis	336:365	the acute experimental colitis mice induced by dextran sulfate sodium (DSS)	336:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	4	50	theme	Body	588:591	arg1	weight					593:598	Body weight	588:598	Body weight	588:598	Body weight, colon length, colon tissue pathology, and colon tissue inflammatory cytokines were assessed.
35655785	0	51	theme	Ulcerative	33:42	arg1	Colitis					44:50	Acute Ulcerative Colitis	27:50	Acute Ulcerative Colitis	27:50	Ginsenoside Rg1 Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Microbial Tryptophan Metabolism.
35655785	5	52	theme	gut	723:725	arg1	microbiota					727:736	gut microbiota	723:736	gut microbiota	723:736	The composition structure of gut microbiota was profiled using 16s rRNA sequencing.
35655785	11	53	theme	UC	1659:1660	arg1	mice					1662:1665	UC mice	1659:1665	UC mice	1659:1665	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	5	54	theme	16s	757:759	arg1	sequencing					766:775	16s rRNA sequencing	757:775	16s rRNA sequencing	757:775	The composition structure of gut microbiota was profiled using 16s rRNA sequencing.
35655785	7	55	theme	colonic	964:970	arg1	injury					972:977	DSS-induced colonic injury	952:977	DSS-induced colonic injury	952:977	The results showed that Rg1 significantly ameliorated DSS-induced colonic injury and colonic inflammation.
35655785	3	56	theme	Acute	448:452	arg1	UC					454:455	Acute UC	448:455	Acute UC	448:455	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	5	57	theme	microbiota	727:736	arg1	structure					710:718	The composition structure	694:718	The composition structure of gut microbiota	694:736	The composition structure of gut microbiota was profiled using 16s rRNA sequencing.
35655785	11	58	from	inflammation	1643:1654	arg1	mice					1662:1665	UC mice	1659:1665	UC mice	1659:1665	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	6	59	from	tryptophan	839:848	arg1	serum					877:881	the serum	873:881	the serum	873:881	Global metabolomic profiling of the feces was performed, and tryptophan and its metabolites in the serum were detected.
35655785	3	60	theme	g	528:528	arg1	Rg1					547:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	3	60	theme	g	528:528	arg1	meanwhile					509:517	meanwhile	509:517	meanwhile	509:517	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	3	61	theme	C57BL/6	472:478	arg1	mice					480:483	C57BL/6 mice	472:483	C57BL/6 mice	472:483	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	11	62	theme	metabolism	1789:1798	arg1	regulation					1723:1732	the regulation	1719:1732	the regulation of gut microbiota composition and microbial tryptophan metabolism	1719:1798	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	6	63	theme	metabolomic	785:795	arg1	profiling					797:805	Global metabolomic profiling	778:805	Global metabolomic profiling of the feces	778:818	Global metabolomic profiling of the feces was performed, and tryptophan and its metabolites in the serum were detected.
35655785	3	64	theme	b.w.	530:533	arg1	Rg1					547:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	2 mg/10 g b.w. ginsenoside Rg1	520:549	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	3	64	theme	b.w.	530:533	arg1	meanwhile					509:517	meanwhile	509:517	meanwhile	509:517	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	11	65	theme	colon	1637:1641	arg1	inflammation					1643:1654	colon inflammation	1637:1654	colon inflammation in UC mice	1637:1665	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	8	66	theme	microbiota	1064:1073	arg1	composition					1075:1085	gut microbiota composition	1060:1085	gut microbiota composition caused by DSS	1060:1099	In addition, Rg1 also partly reversed the imbalance of gut microbiota composition caused by DSS.
35655785	6	67	theme	Global	778:783	arg1	profiling					797:805	Global metabolomic profiling	778:805	Global metabolomic profiling of the feces	778:818	Global metabolomic profiling of the feces was performed, and tryptophan and its metabolites in the serum were detected.
35655785	7	68	theme	DSS-induced	952:962	arg1	injury					972:977	DSS-induced colonic injury	952:977	DSS-induced colonic injury	952:977	The results showed that Rg1 significantly ameliorated DSS-induced colonic injury and colonic inflammation.
35655785	4	69	theme	tissue	649:654	arg1	cytokines					669:677	colon tissue inflammatory cytokines	643:677	colon tissue inflammatory cytokines	643:677	Body weight, colon length, colon tissue pathology, and colon tissue inflammatory cytokines were assessed.
35655785	10	70	from	serum	1385:1389	arg1	levels					1345:1350	the levels	1341:1350	the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide	1341:1487	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	2	71	dep	colitis	359:365	arg1	mice					367:370	mice	367:370	the acute experimental colitis mice induced by dextran sulfate sodium (DSS)	336:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	9	72	theme	isoleucine	1206:1215	arg1	biosynthesis					1217:1228	isoleucine biosynthesis	1206:1228	isoleucine biosynthesis	1206:1228	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	11	73	theme	tryptophan	1778:1787	arg1	metabolism					1789:1798	microbial tryptophan metabolism	1768:1798	microbial tryptophan metabolism	1768:1798	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	1	74	from	disorder	182:189	arg1	tract					215:219	the gastrointestinal tract	194:219	the gastrointestinal tract	194:219	Ulcerative colitis (UC) is a chronic and recurrent inflammatory disorder in the gastrointestinal tract.
35655785	4	75	theme	colon	643:647	arg1	cytokines					669:677	colon tissue inflammatory cytokines	643:677	colon tissue inflammatory cytokines	643:677	Body weight, colon length, colon tissue pathology, and colon tissue inflammatory cytokines were assessed.
35655785	11	76	theme	underlying	1676:1685	arg1	mechanism					1687:1695	the underlying mechanism	1672:1695	the underlying mechanism	1672:1695	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	11	76	theme	underlying	1676:1685	arg1	related					1708:1714	related	1708:1714	related	1708:1714	In conclusion, the study discovered that Rg1 can protect the intestinal barrier and alleviate colon inflammation in UC mice, and the underlying mechanism is closely related to the regulation of gut microbiota composition and microbial tryptophan metabolism.
35655785	2	77	theme	pharmacological	244:258	arg1	effects					260:266	the pharmacological effects	240:266	the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS)	240:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	9	78	theme	Rg1	1102:1104	arg1	intervention					1106:1117	Rg1 intervention	1102:1117	Rg1 intervention	1102:1117	Rg1 intervention can regulate various metabolic pathways of gut microbiota such as valine, leucine, and isoleucine biosynthesis and vitamin B6 metabolism and the most prominent metabolic alteration was tryptophan metabolism.
35655785	3	79	theme	%	491:491	arg1	DSS					493:495	2.5% DSS	488:495	2.5% DSS for 7 days	488:506	Acute UC was induced in C57BL/6 mice by 2.5% DSS for 7 days, meanwhile, 2 mg/10 g b.w. ginsenoside Rg1 was administrated to treat the mice.
35655785	2	80	theme	dextran	383:389	arg1	sulfate					391:397	dextran sulfate sodium (DSS)	383:410	dextran sulfate sodium (DSS)	383:410	Here, we examined the pharmacological effects of ginsenoside Rg1, a natural compound with low bioavailability, on the acute experimental colitis mice induced by dextran sulfate sodium (DSS) and explored underlying mechanisms.
35655785	0	81	theme	Gut	66:68	arg1	Microbiota					70:79	Gut Microbiota	66:79	Gut Microbiota	66:79	Ginsenoside Rg1 Alleviates Acute Ulcerative Colitis by Modulating Gut Microbiota and Microbial Tryptophan Metabolism.
35655785	5	82	theme	rRNA	761:764	arg1	sequencing					766:775	16s rRNA sequencing	757:775	16s rRNA sequencing	757:775	The composition structure of gut microbiota was profiled using 16s rRNA sequencing.
35655785	10	83	from	metabolites	1366:1376	arg1	serum					1385:1389	the serum	1381:1389	the serum	1381:1389	DSS decreased the levels of tryptophan metabolites in the serum, including indole-3-carboxaldehyde, indole-3-lactic acid, 3-indolepropionic acid, and niacinamide and Rg1 can increase the levels of these metabolites.
35655785	6	84	from	metabolites	858:868	arg1	serum					877:881	the serum	873:881	the serum	873:881	Global metabolomic profiling of the feces was performed, and tryptophan and its metabolites in the serum were detected.
35150816	17	0	theme	leaf	2005:2008	arg1	explanation					1986:1996	A comprehensive explanation	1970:1996	A comprehensive explanation of the leaf micromorphology and histochemistry	1970:2043	A comprehensive explanation of the leaf micromorphology and histochemistry was presented.
35150816	21	1	theme	histochemical	2582:2594	arg1	analysis					2596:2603	anatomical and histochemical analysis	2567:2603	anatomical and histochemical analysis	2567:2603	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	19	2	from	inflammation	2174:2185	arg1	model					2232:2236	a carrageenan model	2218:2236	a carrageenan model	2218:2236	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	6	3	theme	anti-hyperalgesic	550:566	arg1	properties					608:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	22	4	theme	present	2610:2616	arg1	study					2618:2622	The present study	2606:2622	The present study	2606:2622	The present study showed that MEPT inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent.
35150816	5	5	theme	P.	428:429	arg1	tomentosa					431:439	P. tomentosa	428:439	P. tomentosa	428:439	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	12	6	from	allodynia	1495:1503	arg1	mice					1531:1534	mice	1531:1534	mice	1531:1534	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	5	7	theme	inflammation	306:317	arg1	treatments					292:301	the popular treatments	280:301	the popular treatments of inflammation and pain	280:326	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	6	8	theme	anti-acetylcholinesterase	523:547	arg1	properties					608:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	2	9	theme	Palicourea	180:189	arg1	tomentosa					191:199	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa	149:199	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa (Aubl.)	149:207	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa (Aubl.)
35150816	21	10	dep	P.	2531:2532	arg1	tomentosa					2534:2542	tomentosa	2534:2542	tomentosa	2534:2542	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	13	11	theme	formalin	1622:1629	arg1	method					1631:1636	the formalin method	1618:1636	the formalin method	1618:1636	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg) was evaluated by the formalin method in mice.
35150816	8	12	theme	resolution	932:941	arg1	UHPLC-HRMS/MS					962:974	UHPLC-HRMS/MS	962:974	UHPLC-HRMS/MS	962:974	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	8	12	theme	resolution	932:941	arg1	spectrometry					948:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry	885:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS)	885:975	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	15	13	theme	terpene	1846:1852	arg1	derivatives					1854:1864	two terpene derivatives	1842:1864	two terpene derivatives	1842:1864	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	15	13	theme	terpene	1846:1852	arg1	alkaloids					1801:1809	two alkaloids	1797:1809	two alkaloids	1797:1809	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	2	14	theme	ETHNOPHARMACOLOGICAL	149:168	arg1	tomentosa					191:199	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa	149:199	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa (Aubl.)	149:207	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa (Aubl.)
35150816	16	15	theme	flavonoid	1887:1895	arg1	concentrations					1928:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	12	16	theme	cold	1490:1493	arg1	acetone					1506:1512	acetone	1506:1512	acetone	1506:1512	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	16	theme	cold	1490:1493	arg1	allodynia					1495:1503	cold allodynia	1490:1503	cold allodynia (acetone)	1490:1513	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	16	theme	cold	1490:1493	arg1	models					1432:1437	carrageenan-induced models	1412:1437	carrageenan-induced models of paw oedema	1412:1451	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	21	17	theme	anatomical	2567:2576	arg1	analysis					2596:2603	anatomical and histochemical analysis	2567:2603	anatomical and histochemical analysis	2567:2603	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	12	18	theme	oedema	1446:1451	arg1	pleurisy					1519:1526	pleurisy	1519:1526	pleurisy in mice	1519:1534	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	18	theme	oedema	1446:1451	arg1	allodynia					1495:1503	cold allodynia	1490:1503	cold allodynia (acetone)	1490:1513	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	18	theme	oedema	1446:1451	arg1	models					1432:1437	carrageenan-induced models	1412:1437	carrageenan-induced models of paw oedema	1412:1451	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	18	theme	oedema	1446:1451	arg1	hyperalgesia					1465:1476	mechanical hyperalgesia	1454:1476	mechanical hyperalgesia (Von Frey)	1454:1487	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	9	19	theme	flavonols	1027:1035	arg1	concentrations					982:995	The concentrations	978:995	The concentrations of total phenols, flavonoids, flavonols and condensed tannin	978:1056	The concentrations of total phenols, flavonoids, flavonols and condensed tannin were determined.
35150816	20	20	theme	oral	2349:2352	arg1	administration					2354:2367	The oral administration	2345:2367	The oral administration of 30 and 100 mg/kg MEPT	2345:2392	The oral administration of 30 and 100 mg/kg MEPT significantly inhibited AChE activity in the frontal cortex.
35150816	16	21	theme	flavonol	1898:1905	arg1	concentrations					1928:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	12	22	theme	mechanical	1454:1463	arg1	models					1432:1437	carrageenan-induced models	1412:1437	carrageenan-induced models of paw oedema	1412:1451	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	22	theme	mechanical	1454:1463	arg1	hyperalgesia					1465:1476	mechanical hyperalgesia	1454:1476	mechanical hyperalgesia (Von Frey)	1454:1487	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	22	theme	mechanical	1454:1463	arg1	Frey					1483:1486	Von Frey	1479:1486	Von Frey	1479:1486	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	9	23	theme	condensed	1041:1049	arg1	tannin					1051:1056	condensed tannin	1041:1056	condensed tannin	1041:1056	The concentrations of total phenols, flavonoids, flavonols and condensed tannin were determined.
35150816	9	24	theme	flavonoids	1015:1024	arg1	concentrations					982:995	The concentrations	978:995	The concentrations of total phenols, flavonoids, flavonols and condensed tannin	978:1056	The concentrations of total phenols, flavonoids, flavonols and condensed tannin were determined.
35150816	16	25	theme	condensed	1911:1919	arg1	tannin					1921:1926	condensed tannin	1911:1926	condensed tannin	1911:1926	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	12	26	theme	carrageenan-induced	1412:1430	arg1	pleurisy					1519:1526	pleurisy	1519:1526	pleurisy in mice	1519:1534	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	26	theme	carrageenan-induced	1412:1430	arg1	allodynia					1495:1503	cold allodynia	1490:1503	cold allodynia (acetone)	1490:1513	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	26	theme	carrageenan-induced	1412:1430	arg1	models					1432:1437	carrageenan-induced models	1412:1437	carrageenan-induced models of paw oedema	1412:1451	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	26	theme	carrageenan-induced	1412:1430	arg1	hyperalgesia					1465:1476	mechanical hyperalgesia	1454:1476	mechanical hyperalgesia (Von Frey)	1454:1487	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	20	27	theme	MEPT	2389:2392	arg1	administration					2354:2367	The oral administration	2345:2367	The oral administration of 30 and 100 mg/kg MEPT	2345:2392	The oral administration of 30 and 100 mg/kg MEPT significantly inhibited AChE activity in the frontal cortex.
35150816	19	28	theme	formalin-induced	2250:2265	arg1	nociception					2267:2277	formalin-induced nociception	2250:2277	formalin-induced nociception	2250:2277	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	17	29	theme	comprehensive	1972:1984	arg1	explanation					1986:1996	A comprehensive explanation	1970:1996	A comprehensive explanation of the leaf micromorphology and histochemistry	1970:2043	A comprehensive explanation of the leaf micromorphology and histochemistry was presented.
35150816	5	30	theme	pain	323:326	arg1	treatments					292:301	the popular treatments	280:301	the popular treatments of inflammation and pain	280:326	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	6	31	dep	composition	644:654	arg1	leaves					733:738	leaves	733:738	leaves	733:738	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	6	32	theme	anti-inflammatory	590:606	arg1	properties					608:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	14	33	theme	rat	1719:1721	arg1	structures					1729:1738	four rat brain structures	1714:1738	four rat brain structures	1714:1738	The anti-acetylcholinesterase properties were evaluated in vivo in four rat brain structures.
35150816	8	34	theme	METHODS	852:858	arg1	MEPT					864:867	MATERIALS AND METHODS The MEPT	838:867	MATERIALS AND METHODS The MEPT	838:867	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	21	35	dep	CONCLUSION	2455:2464	arg1	This					2466:2469	This	2466:2469	This	2466:2469	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	10	36	theme	leaves	1117:1122	arg1	histochemistry					1099:1112	histochemistry	1099:1112	histochemistry	1099:1112	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	10	36	theme	leaves	1117:1122	arg1	micromorphology					1079:1093	micromorphology	1079:1093	micromorphology	1079:1093	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	15	37	theme	MEPT	1779:1782	arg1	chromatogram					1763:1774	The total ion chromatogram	1749:1774	RESULTS The total ion chromatogram of MEPT	1741:1782	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	9	38	theme	phenols	1006:1012	arg1	concentrations					982:995	The concentrations	978:995	The concentrations of total phenols, flavonoids, flavonols and condensed tannin	978:1056	The concentrations of total phenols, flavonoids, flavonols and condensed tannin were determined.
35150816	21	39	theme	chemical	2484:2491	arg1	study					2508:2512	the first chemical and biological study	2474:2512	the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis	2474:2603	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	21	39	theme	chemical	2484:2491	arg1	CONCLUSION					2455:2464	CONCLUSION	2455:2464	CONCLUSION This	2455:2469	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	7	40	theme	P.	824:825	arg1	tomentosa					827:835	P. tomentosa	824:835	P. tomentosa	824:835	The study also analyzes the micromorphology and histochemistry of leaves of P. tomentosa.
35150816	6	41	theme	STUDY	449:453	arg1	AIM					442:444	AIM	442:444	AIM OF STUDY	442:453	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	10	42	theme	standard	1145:1152	arg1	reagents					1154:1161	standard reagents	1145:1161	standard reagents	1145:1161	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	10	42	theme	standard	1145:1152	arg1	microscopy					1207:1216	field emission scanning electron microscopy	1174:1216	field emission scanning electron microscopy	1174:1216	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	10	42	theme	standard	1145:1152	arg1	light					1164:1168	light	1164:1168	light	1164:1168	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	19	43	theme	cold	2306:2309	arg1	sensitivity					2311:2321	cold sensitivity	2306:2321	cold sensitivity	2306:2321	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	16	44	theme	phenol	1879:1884	arg1	concentrations					1928:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	8	45	theme	ultra-high-pressure	885:903	arg1	UHPLC-HRMS/MS					962:974	UHPLC-HRMS/MS	962:974	UHPLC-HRMS/MS	962:974	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	8	45	theme	ultra-high-pressure	885:903	arg1	spectrometry					948:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry	885:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS)	885:975	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	15	46	theme	total	1753:1757	arg1	chromatogram					1763:1774	The total ion chromatogram	1749:1774	RESULTS The total ion chromatogram of MEPT	1741:1782	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	8	47	theme	MATERIALS	838:846	arg1	MEPT					864:867	MATERIALS AND METHODS The MEPT	838:867	MATERIALS AND METHODS The MEPT	838:867	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	10	48	theme	emission	1180:1187	arg1	microscopy					1207:1216	field emission scanning electron microscopy	1174:1216	field emission scanning electron microscopy	1174:1216	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	10	48	theme	emission	1180:1187	arg1	reagents					1154:1161	standard reagents	1145:1161	standard reagents	1145:1161	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	8	49	theme	chromatography-high	912:930	arg1	UHPLC-HRMS/MS					962:974	UHPLC-HRMS/MS	962:974	UHPLC-HRMS/MS	962:974	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	8	49	theme	chromatography-high	912:930	arg1	spectrometry					948:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry	885:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS)	885:975	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	19	50	from	parameters	2204:2213	arg1	model					2232:2236	a carrageenan model	2218:2236	a carrageenan model	2218:2236	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	23	51	theme	species	2908:2914	arg1	identification					2916:2929	species identification	2908:2929	species identification	2908:2929	Also, anatomical and histochemistry of leaves described in the present study provide microscopical information, which aids species identification.
35150816	8	52	theme	mass	943:946	arg1	UHPLC-HRMS/MS					962:974	UHPLC-HRMS/MS	962:974	UHPLC-HRMS/MS	962:974	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	8	52	theme	mass	943:946	arg1	spectrometry					948:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry	885:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS)	885:975	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	23	53	theme	leaves	2824:2829	arg1	histochemistry					2806:2819	histochemistry	2806:2819	histochemistry	2806:2819	Also, anatomical and histochemistry of leaves described in the present study provide microscopical information, which aids species identification.
35150816	23	53	theme	leaves	2824:2829	arg1	anatomical					2791:2800	anatomical	2791:2800	anatomical	2791:2800	Also, anatomical and histochemistry of leaves described in the present study provide microscopical information, which aids species identification.
35150816	0	54	theme	Palicourea	0:9	arg1	tomentosa					11:19	Palicourea tomentosa	0:19	Palicourea tomentosa (Aubl.)	0:27	Palicourea tomentosa (Aubl.)
35150816	6	55	dep	undertaken	470:479	arg1	AIM					442:444	AIM	442:444	AIM OF STUDY	442:453	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	6	56	theme	potential	500:508	arg1	properties					608:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	15	57	theme	coumarin	1816:1823	arg1	alkaloids					1801:1809	two alkaloids	1797:1809	two alkaloids	1797:1809	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	15	57	theme	coumarin	1816:1823	arg1	iridoid					1830:1836	one coumarin, one iridoid	1812:1836	iridoid	1830:1836	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	10	58	theme	electron	1198:1205	arg1	microscopy					1207:1216	field emission scanning electron microscopy	1174:1216	field emission scanning electron microscopy	1174:1216	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	10	58	theme	electron	1198:1205	arg1	reagents					1154:1161	standard reagents	1145:1161	standard reagents	1145:1161	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	18	59	theme	MDA	2121:2123	arg1	models					2125:2130	the DPPH, β-carotene and MDA models	2096:2130	the DPPH, β-carotene and MDA models	2096:2130	MEPT was significantly inhibited by the DPPH, β-carotene and MDA models.
35150816	21	60	theme	biological	2497:2506	arg1	study					2508:2512	the first chemical and biological study	2474:2512	the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis	2474:2603	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	21	60	theme	biological	2497:2506	arg1	CONCLUSION					2455:2464	CONCLUSION	2455:2464	CONCLUSION This	2455:2469	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	6	61	theme	extract	709:715	arg1	constituents					678:689	constituents	678:689	constituents of the methanolic extract of P. tomentosa	678:731	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	7	62	theme	leaves	814:819	arg1	histochemistry					796:809	histochemistry	796:809	histochemistry	796:809	The study also analyzes the micromorphology and histochemistry of leaves of P. tomentosa.
35150816	7	62	theme	leaves	814:819	arg1	micromorphology					776:790	micromorphology	776:790	micromorphology	776:790	The study also analyzes the micromorphology and histochemistry of leaves of P. tomentosa.
35150816	7	63	dep	micromorphology	776:790	arg1	the					772:774	the	772:774	the	772:774	The study also analyzes the micromorphology and histochemistry of leaves of P. tomentosa.
35150816	6	64	theme	antioxidant	510:520	arg1	properties					608:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	23	65	theme	present	2848:2854	arg1	study					2856:2860	the present study	2844:2860	the present study	2844:2860	Also, anatomical and histochemistry of leaves described in the present study provide microscopical information, which aids species identification.
35150816	10	66	theme	energy-dispersive	1226:1242	arg1	spectroscopy					1250:1261	energy-dispersive X-ray spectroscopy	1226:1261	energy-dispersive X-ray spectroscopy	1226:1261	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	16	67	dep	phenol	1879:1884	arg1	The					1867:1869	The	1867:1869	The	1867:1869	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	22	68	theme	analgesic	2760:2768	arg1	agent					2778:2782	analgesic natural agent	2760:2782	analgesic natural agent	2760:2782	The present study showed that MEPT inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent.
35150816	16	69	located	found	1948:1952	arg1	extract					1961:1967	the extract	1957:1967	the extract	1957:1967	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	16	69	located	found	1948:1952	arg2	concentrations					1928:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	12	70	theme	anti-inflammatory	1337:1353	arg1	activity					1355:1362	The anti-inflammatory activity	1333:1362	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg)	1333:1395	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	19	71	from	nociception	2267:2277	arg1	phases					2287:2292	both phases	2282:2292	both phases	2282:2292	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	15	72	dep	RESULTS	1741:1747	arg1	chromatogram					1763:1774	The total ion chromatogram	1749:1774	RESULTS The total ion chromatogram of MEPT	1741:1782	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	5	73	theme	popular	284:290	arg1	treatments					292:301	the popular treatments	280:301	the popular treatments of inflammation and pain	280:326	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	5	74	theme	methanolic	406:415	arg1	extract					417:423	the methanolic extract	402:423	the methanolic extract of P. tomentosa	402:439	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	5	74	theme	methanolic	406:415	arg1	activity					390:397	their activity	384:397	their activity	384:397	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	18	75	theme	β-carotene	2106:2115	arg1	models					2125:2130	the DPPH, β-carotene and MDA models	2096:2130	the DPPH, β-carotene and MDA models	2096:2130	MEPT was significantly inhibited by the DPPH, β-carotene and MDA models.
35150816	2	76	dep	tomentosa	191:199	arg1	Aubl					202:205	Aubl	202:205	Aubl.	202:206	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa (Aubl.)
35150816	9	77	theme	total	1000:1004	arg1	phenols					1006:1012	total phenols	1000:1012	total phenols	1000:1012	The concentrations of total phenols, flavonoids, flavonols and condensed tannin were determined.
35150816	5	78	theme	tomentosa	431:439	arg1	extract					417:423	the methanolic extract	402:423	the methanolic extract of P. tomentosa	402:439	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	5	78	theme	tomentosa	431:439	arg1	activity					390:397	their activity	384:397	their activity	384:397	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	18	79	theme	DPPH	2100:2103	arg1	models					2125:2130	the DPPH, β-carotene and MDA models	2096:2130	the DPPH, β-carotene and MDA models	2096:2130	MEPT was significantly inhibited by the DPPH, β-carotene and MDA models.
35150816	2	80	theme	RELEVANCE	170:178	arg1	tomentosa					191:199	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa	149:199	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa (Aubl.)	149:207	ETHNOPHARMACOLOGICAL RELEVANCE Palicourea tomentosa (Aubl.)
35150816	10	81	dep	micromorphology	1079:1093	arg1	The					1075:1077	The	1075:1077	The	1075:1077	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	12	82	theme	Von	1479:1481	arg1	hyperalgesia					1465:1476	mechanical hyperalgesia	1454:1476	mechanical hyperalgesia (Von Frey)	1454:1487	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	82	theme	Von	1479:1481	arg1	Frey					1483:1486	Von Frey	1479:1486	Von Frey	1479:1486	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	6	83	theme	chemical	635:642	arg1	composition					644:654	the chemical composition	631:654	the chemical composition	631:654	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	6	83	theme	chemical	635:642	arg1	MEPT					741:744	MEPT	741:744	MEPT	741:744	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	12	84	theme	paw	1442:1444	arg1	oedema					1446:1451	paw oedema	1442:1451	paw oedema	1442:1451	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	17	85	dep	leaf	2005:2008	arg1	histochemistry					2030:2043	histochemistry	2030:2043	histochemistry	2030:2043	A comprehensive explanation of the leaf micromorphology and histochemistry was presented.
35150816	17	85	dep	leaf	2005:2008	arg1	micromorphology					2010:2024	micromorphology	2010:2024	micromorphology	2010:2024	A comprehensive explanation of the leaf micromorphology and histochemistry was presented.
35150816	16	86	theme	tannin	1921:1926	arg1	concentrations					1928:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations	1867:1941	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	9	87	theme	tannin	1051:1056	arg1	concentrations					982:995	The concentrations	978:995	The concentrations of total phenols, flavonoids, flavonols and condensed tannin	978:1056	The concentrations of total phenols, flavonoids, flavonols and condensed tannin were determined.
35150816	19	88	theme	carrageenan	2220:2230	arg1	model					2232:2236	a carrageenan model	2218:2236	a carrageenan model	2218:2236	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	14	89	theme	brain	1723:1727	arg1	structures					1729:1738	four rat brain structures	1714:1738	four rat brain structures	1714:1738	The anti-acetylcholinesterase properties were evaluated in vivo in four rat brain structures.
35150816	12	90	dep	MEPT	1367:1370	arg1	100					1377:1379	100	1377:1379	100	1377:1379	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	12	90	dep	MEPT	1367:1370	arg1	300 mg/kg					1386:1394	300 mg/kg	1386:1394	300 mg/kg	1386:1394	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	1	91	theme	anti-inflammatory	90:106	arg1	potential					138:146	the analgesic, anti-inflammatory and anti-acetylcholinesterase potential	75:146	the analgesic, anti-inflammatory and anti-acetylcholinesterase potential	75:146	Borhidi: Microscopy, chemical composition and the analgesic, anti-inflammatory and anti-acetylcholinesterase potential.
35150816	12	92	from	pleurisy	1519:1526	arg1	mice					1531:1534	mice	1531:1534	mice	1531:1534	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	19	93	theme	hyperalgesic	2191:2202	arg1	parameters					2204:2213	hyperalgesic parameters	2191:2213	hyperalgesic parameters	2191:2213	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	6	94	theme	methanolic	698:707	arg1	extract					709:715	the methanolic extract	694:715	the methanolic extract of P. tomentosa	694:731	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	22	95	theme	inflammatory	2660:2671	arg1	parameters					2673:2682	inflammatory parameters	2660:2682	inflammatory parameters	2660:2682	The present study showed that MEPT inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent.
35150816	13	96	theme	MEPT	1571:1574	arg1	potential					1558:1566	The anti-nociceptive potential	1537:1566	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg)	1537:1599	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg) was evaluated by the formalin method in mice.
35150816	1	97	theme	anti-acetylcholinesterase	112:136	arg1	potential					138:146	the analgesic, anti-inflammatory and anti-acetylcholinesterase potential	75:146	the analgesic, anti-inflammatory and anti-acetylcholinesterase potential	75:146	Borhidi: Microscopy, chemical composition and the analgesic, anti-inflammatory and anti-acetylcholinesterase potential.
35150816	16	98	theme	highest	1871:1877	arg1	phenol					1879:1884	phenol	1879:1884	phenol	1879:1884	The highest phenol, flavonoid, flavonol and condensed tannin concentrations were found in the extract.
35150816	21	99	theme	first	2478:2482	arg1	study					2508:2512	the first chemical and biological study	2474:2512	the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis	2474:2603	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	21	99	theme	first	2478:2482	arg1	CONCLUSION					2455:2464	CONCLUSION	2455:2464	CONCLUSION This	2455:2469	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	6	100	theme	constituents	678:689	arg1	concentrations					660:673	concentrations	660:673	concentrations of constituents of the methanolic extract of P. tomentosa	660:731	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	6	100	theme	constituents	678:689	arg1	composition					644:654	the chemical composition	631:654	the chemical composition	631:654	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	6	100	theme	constituents	678:689	arg1	MEPT					741:744	MEPT	741:744	MEPT	741:744	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	15	101	theme	ion	1759:1761	arg1	chromatogram					1763:1774	The total ion chromatogram	1749:1774	RESULTS The total ion chromatogram of MEPT	1741:1782	RESULTS The total ion chromatogram of MEPT demonstrated two alkaloids, one coumarin, one iridoid and two terpene derivatives.
35150816	22	102	theme	popular	2731:2737	arg1	use					2739:2741	the popular use	2727:2741	the popular use of this plant as analgesic natural agent	2727:2782	The present study showed that MEPT inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent.
35150816	14	103	theme	anti-acetylcholinesterase	1651:1675	arg1	properties					1677:1686	The anti-acetylcholinesterase properties	1647:1686	The anti-acetylcholinesterase properties	1647:1686	The anti-acetylcholinesterase properties were evaluated in vivo in four rat brain structures.
35150816	5	104	theme	scientific	351:360	arg1	studies					362:368	no scientific studies	348:368	no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa	348:439	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	20	105	theme	frontal	2439:2445	arg1	cortex					2447:2452	the frontal cortex	2435:2452	the frontal cortex	2435:2452	The oral administration of 30 and 100 mg/kg MEPT significantly inhibited AChE activity in the frontal cortex.
35150816	11	106	theme	antioxidant	1268:1278	arg1	activity					1280:1287	The antioxidant activity	1264:1287	The antioxidant activity	1264:1287	The antioxidant activity was evaluated for DPPH, β-carotene and MDA.
35150816	10	107	theme	field	1174:1178	arg1	microscopy					1207:1216	field emission scanning electron microscopy	1174:1216	field emission scanning electron microscopy	1174:1216	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	10	107	theme	field	1174:1178	arg1	reagents					1154:1161	standard reagents	1145:1161	standard reagents	1145:1161	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	1	108	theme	chemical	50:57	arg1	composition					59:69	chemical composition	50:69	chemical composition	50:69	Borhidi: Microscopy, chemical composition and the analgesic, anti-inflammatory and anti-acetylcholinesterase potential.
35150816	8	109	theme	liquid	905:910	arg1	UHPLC-HRMS/MS					962:974	UHPLC-HRMS/MS	962:974	UHPLC-HRMS/MS	962:974	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	8	109	theme	liquid	905:910	arg1	spectrometry					948:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry	885:959	ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS)	885:975	MATERIALS AND METHODS The MEPT was analysed by ultra-high-pressure liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS/MS).
35150816	13	110	theme	anti-nociceptive	1541:1556	arg1	potential					1558:1566	The anti-nociceptive potential	1537:1566	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg)	1537:1599	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg) was evaluated by the formalin method in mice.
35150816	20	111	theme	AChE	2418:2421	arg1	activity					2423:2430	AChE activity	2418:2430	AChE activity	2418:2430	The oral administration of 30 and 100 mg/kg MEPT significantly inhibited AChE activity in the frontal cortex.
35150816	19	112	dep	MEPT	2133:2136	arg1	100					2143:2145	100	2143:2145	100	2143:2145	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	19	112	dep	MEPT	2133:2136	arg1	30					2139:2140	30	2139:2140	30	2139:2140	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	19	112	dep	MEPT	2133:2136	arg1	300 mg/kg					2151:2159	300 mg/kg	2151:2159	300 mg/kg	2151:2159	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	12	113	from	hyperalgesia	1465:1476	arg1	mice					1531:1534	mice	1531:1534	mice	1531:1534	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	21	114	theme	P.	2531:2532	arg1	extract					2555:2561	a P. tomentosa methanolic extract	2529:2561	a P. tomentosa methanolic extract	2529:2561	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	22	115	theme	plant	2751:2755	arg1	use					2739:2741	the popular use	2727:2741	the popular use of this plant as analgesic natural agent	2727:2782	The present study showed that MEPT inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent.
35150816	6	116	theme	tomentosa	723:731	arg1	extract					709:715	the methanolic extract	694:715	the methanolic extract of P. tomentosa	694:731	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
35150816	19	117	theme	oedema	2327:2332	arg1	formation					2334:2342	oedema formation	2327:2342	oedema formation	2327:2342	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	1	118	theme	analgesic	79:87	arg1	potential					138:146	the analgesic, anti-inflammatory and anti-acetylcholinesterase potential	75:146	the analgesic, anti-inflammatory and anti-acetylcholinesterase potential	75:146	Borhidi: Microscopy, chemical composition and the analgesic, anti-inflammatory and anti-acetylcholinesterase potential.
35150816	12	119	theme	MEPT	1367:1370	arg1	activity					1355:1362	The anti-inflammatory activity	1333:1362	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg)	1333:1395	The anti-inflammatory activity of MEPT (30, 100, and 300 mg/kg) was assayed in carrageenan-induced models of paw oedema, mechanical hyperalgesia (Von Frey), cold allodynia (acetone) and pleurisy in mice.
35150816	21	120	theme	methanolic	2544:2553	arg1	extract					2555:2561	a P. tomentosa methanolic extract	2529:2561	a P. tomentosa methanolic extract	2529:2561	CONCLUSION This is the first chemical and biological study performed with a P. tomentosa methanolic extract and anatomical and histochemical analysis.
35150816	10	121	theme	scanning	1189:1196	arg1	microscopy					1207:1216	field emission scanning electron microscopy	1174:1216	field emission scanning electron microscopy	1174:1216	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	10	121	theme	scanning	1189:1196	arg1	reagents					1154:1161	standard reagents	1145:1161	standard reagents	1145:1161	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	13	122	dep	MEPT	1571:1574	arg1	30					1577:1578	30	1577:1578	30	1577:1578	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg) was evaluated by the formalin method in mice.
35150816	13	122	dep	MEPT	1571:1574	arg1	100					1581:1583	100	1581:1583	100	1581:1583	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg) was evaluated by the formalin method in mice.
35150816	13	122	dep	MEPT	1571:1574	arg1	300 mg/kg					1590:1598	300 mg/kg	1590:1598	300 mg/kg	1590:1598	The anti-nociceptive potential of MEPT (30, 100, and 300 mg/kg) was evaluated by the formalin method in mice.
35150816	23	123	theme	microscopical	2870:2882	arg1	information					2884:2894	microscopical information	2870:2894	microscopical information	2870:2894	Also, anatomical and histochemistry of leaves described in the present study provide microscopical information, which aids species identification.
35150816	10	124	theme	X-ray	1244:1248	arg1	spectroscopy					1250:1261	energy-dispersive X-ray spectroscopy	1226:1261	energy-dispersive X-ray spectroscopy	1226:1261	The micromorphology and histochemistry of leaves were performed using standard reagents, light and field emission scanning electron microscopy, beyond energy-dispersive X-ray spectroscopy.
35150816	22	125	theme	natural	2770:2776	arg1	agent					2778:2782	analgesic natural agent	2760:2782	analgesic natural agent	2760:2782	The present study showed that MEPT inhibited pain and inflammatory parameters contributing, at least in part, to explain the popular use of this plant as analgesic natural agent.
35150816	19	126	dep	inflammation	2174:2185	arg1	the					2170:2172	the	2170:2172	the	2170:2172	MEPT (30, 100 and 300 mg/kg) reduced the inflammation and hyperalgesic parameters in a carrageenan model and reduced formalin-induced nociception in both phases, which were cold sensitivity and oedema formation.
35150816	0	127	dep	tomentosa	11:19	arg1	Aubl					22:25	Aubl	22:25	Aubl.	22:26	Palicourea tomentosa (Aubl.)
35150816	5	128	used	used	272:275	arg2	leaves					261:266	leaves	261:266	leaves	261:266	leaves are used in the popular treatments of inflammation and pain; however, there are no scientific studies demonstrating their activity as the methanolic extract of P. tomentosa.
35150816	6	129	theme	anti-nociceptive	569:584	arg1	properties					608:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties	496:617	AIM OF STUDY This study was undertaken to investigate the potential antioxidant, anti-acetylcholinesterase, anti-hyperalgesic, anti-nociceptive and anti-inflammatory properties, as well as the chemical composition and concentrations of constituents of the methanolic extract of P. tomentosa leaves (MEPT).
36260499	4	0	theme	genus	610:614	arg1	Tabrizicola					616:626	the genus Tabrizicola	606:626	the genus Tabrizicola	606:626	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	3	1	theme	irregular	379:387	arg1	colonies					389:396	milky white irregular colonies	367:396	milky white irregular colonies on Reasoner's 2A agar medium	367:425	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36260499	9	2	dep	identity	1049:1056	arg1	%					1065:1065	73.56 %	1059:1065	73.56 % for ANIb between strain J26T with RCRI19T	1059:1107	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	4	3	theme	16S	459:461	arg1	sequences					473:481	16S rRNA gene sequences	459:481	16S rRNA gene sequences	459:481	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	5	4	theme	Cellular	629:636	arg1	components					638:647	Cellular components	629:647	Cellular components of J26T	629:655	Cellular components of J26T supported this strain as a member of the genus Tabrizicola.
36260499	9	5	theme	16S	992:994	arg1	similarity					1015:1024	low 16S rRNA gene sequence similarity	988:1024	low 16S rRNA gene sequence similarity	988:1024	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	9	6	theme	strain	1084:1089	arg1	J26T					1091:1094	strain J26T	1084:1094	strain J26T with RCRI19T	1084:1107	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	9	7	theme	rRNA	996:999	arg1	similarity					1015:1024	low 16S rRNA gene sequence similarity	988:1024	low 16S rRNA gene sequence similarity	988:1024	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	12	8	theme	=GDMCC	1480:1485	arg1	92112T					1500:1505	=GDMCC 1.2843T=KCTC 92112T	1480:1505	=GDMCC 1.2843T=KCTC 92112T	1480:1505	The type strain is J26T (=GDMCC 1.2843T=KCTC 92112T).
36260499	12	8	theme	=GDMCC	1480:1485	arg1	J26T					1474:1477	J26T	1474:1477	J26T (=GDMCC 1.2843T=KCTC 92112T)	1474:1506	The type strain is J26T (=GDMCC 1.2843T=KCTC 92112T).
36260499	6	9	theme	predominant	721:731	arg1	C18 					750:753	C18 	750:753	C18 	750:753	The predominant fatty acids were C18 : 1 ω7c, C18 : 1 ω7c-11 methyl and C16 : 0.
36260499	6	9	theme	predominant	721:731	arg1	acids					739:743	The predominant fatty acids	717:743	The predominant fatty acids	717:743	The predominant fatty acids were C18 : 1 ω7c, C18 : 1 ω7c-11 methyl and C16 : 0.
36260499	8	10	theme	Ubiquinone	889:898	arg1	quinone					931:937	the major respiratory quinone	909:937	the major respiratory quinone	909:937	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	8	10	theme	Ubiquinone	889:898	arg1	Q-10					900:903	Ubiquinone Q-10	889:903	Ubiquinone Q-10	889:903	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	9	11	theme	gene	1001:1004	arg1	similarity					1015:1024	low 16S rRNA gene sequence similarity	988:1024	low 16S rRNA gene sequence similarity	988:1024	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	3	12	theme	agar	415:418	arg1	medium					420:425	Reasoner's 2A agar medium	401:425	Reasoner's 2A agar medium	401:425	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36260499	1	13	from	sediment	53:60	arg1	Foshan					85:90	Foshan	85:90	Foshan	85:90	nov., isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	1	13	from	sediment	53:60	arg1	China					98:102	China	98:102	China	98:102	nov., isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	4	14	theme	gene	468:471	arg1	sequences					473:481	16S rRNA gene sequences	459:481	16S rRNA gene sequences	459:481	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	10	15	theme	novel	1374:1378	arg1	species					1380:1386	a novel species	1372:1386	a novel species	1372:1386	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	2	16	theme	Gram-negative	113:125	arg1	bacterium					182:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	3	17	dep	%	316:316	arg1	w/v					319:321	w/v	319:321	w/v	319:321	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36260499	9	18	theme	sequence	1006:1013	arg1	similarity					1015:1024	low 16S rRNA gene sequence similarity	988:1024	low 16S rRNA gene sequence similarity	988:1024	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	0	19	theme	Tabrizicola	0:10	arg1	sp					25:26	Tabrizicola rongguiensis sp	0:26	Tabrizicola rongguiensis sp.	0:27	Tabrizicola rongguiensis sp.
36260499	4	20	theme	rRNA	463:466	arg1	sequences					473:481	16S rRNA gene sequences	459:481	16S rRNA gene sequences	459:481	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	2	21	theme	novel	107:111	arg1	bacterium					182:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	9	22	with	J26T	1091:1094	arg1	RCRI19T					1101:1107	RCRI19T	1101:1107	RCRI19T	1101:1107	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	5	23	theme	Tabrizicola	704:714	arg1	strain					672:677	this strain	667:677	this strain	667:677	Cellular components of J26T supported this strain as a member of the genus Tabrizicola.
36260499	5	23	theme	Tabrizicola	704:714	arg1	member					684:689	a member	682:689	a member of the genus Tabrizicola	682:714	Cellular components of J26T supported this strain as a member of the genus Tabrizicola.
36260499	3	24	theme	Strain	284:289	arg1	J26T					291:294	Strain J26T	284:294	Strain J26T	284:294	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36260499	3	25	theme	2A	412:413	arg1	medium					420:425	Reasoner's 2A agar medium	401:425	Reasoner's 2A agar medium	401:425	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36260499	0	26	theme	rongguiensis	12:23	arg1	sp					25:26	Tabrizicola rongguiensis sp	0:26	Tabrizicola rongguiensis sp.	0:27	Tabrizicola rongguiensis sp.
36260499	6	27	theme	fatty	733:737	arg1	C18 					750:753	C18 	750:753	C18 	750:753	The predominant fatty acids were C18 : 1 ω7c, C18 : 1 ω7c-11 methyl and C16 : 0.
36260499	6	27	theme	fatty	733:737	arg1	acids					739:743	The predominant fatty acids	717:743	The predominant fatty acids	717:743	The predominant fatty acids were C18 : 1 ω7c, C18 : 1 ω7c-11 methyl and C16 : 0.
36260499	8	28	theme	major	913:917	arg1	quinone					931:937	the major respiratory quinone	909:937	the major respiratory quinone	909:937	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	8	28	theme	major	913:917	arg1	Q-10					900:903	Ubiquinone Q-10	889:903	Ubiquinone Q-10	889:903	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	9	29	theme	average	1030:1036	arg1	identity					1049:1056	average nucleotide identity	1030:1056	average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T)	1030:1108	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	1	30	theme	river	67:71	arg1	sediment					53:60	the sediment	49:60	the sediment of a river in Ronggui, Foshan city, China	49:102	nov., isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	9	31	theme	strain	1128:1133	arg1	J26T					1135:1138	strain J26T	1128:1138	strain J26T	1128:1138	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	3	32	theme	%	316:316	arg1	NaCl					324:327	0 % (w/v) NaCl	314:327	0 % (w/v) NaCl	314:327	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36260499	4	33	theme	Phylogenetic	428:439	arg1	analysis					441:448	Phylogenetic analysis	428:448	Phylogenetic analysis based on 16S rRNA gene sequences	428:481	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	10	34	theme	biochemical	1260:1270	arg1	properties					1272:1281	physiological and biochemical properties	1242:1281	physiological and biochemical properties	1242:1281	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	10	34	theme	biochemical	1260:1270	arg1	nitrogen					1300:1307	nitrogen	1300:1307	nitrogen	1300:1307	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	10	34	theme	biochemical	1260:1270	arg1	metabolism					1321:1330	sulphur metabolism	1313:1330	sulphur metabolism	1313:1330	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	10	34	theme	biochemical	1260:1270	arg1	carbon					1292:1297	carbon	1292:1297	carbon	1292:1297	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	9	35	theme	nucleotide	1038:1047	arg1	identity					1049:1056	average nucleotide identity	1030:1056	average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T)	1030:1108	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	4	36	theme	distinct	588:595	arg1	clade					597:601	a distinct clade	586:601	a distinct clade	586:601	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	10	37	theme	physiological	1242:1254	arg1	properties					1272:1281	physiological and biochemical properties	1242:1281	physiological and biochemical properties	1242:1281	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	10	37	theme	physiological	1242:1254	arg1	nitrogen					1300:1307	nitrogen	1300:1307	nitrogen	1300:1307	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	10	37	theme	physiological	1242:1254	arg1	metabolism					1321:1330	sulphur metabolism	1313:1330	sulphur metabolism	1313:1330	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	10	37	theme	physiological	1242:1254	arg1	carbon					1292:1297	carbon	1292:1297	carbon	1292:1297	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	8	38	theme	respiratory	919:929	arg1	quinone					931:937	the major respiratory quinone	909:937	the major respiratory quinone	909:937	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	8	38	theme	respiratory	919:929	arg1	Q-10					900:903	Ubiquinone Q-10	889:903	Ubiquinone Q-10	889:903	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	2	39	theme	non-spore-forming	137:153	arg1	bacterium					182:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	2	40	theme	river	246:250	arg1	sediment					232:239	the sediment	228:239	the sediment of a river in Ronggui, Foshan city, China	228:281	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	12	41	theme	type	1459:1462	arg1	J26T					1474:1477	J26T	1474:1477	J26T (=GDMCC 1.2843T=KCTC 92112T)	1474:1506	The type strain is J26T (=GDMCC 1.2843T=KCTC 92112T).
36260499	12	41	theme	type	1459:1462	arg1	strain					1464:1469	The type strain	1455:1469	The type strain	1455:1469	The type strain is J26T (=GDMCC 1.2843T=KCTC 92112T).
36260499	4	42	theme	highest	515:521	arg1	similarity					523:532	the highest similarity	511:532	the highest similarity	511:532	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	10	43	theme	rongguiensis	1420:1431	arg1	sp					1433:1434	the name Tabrizicola rongguiensis sp	1399:1434	the name Tabrizicola rongguiensis sp	1399:1434	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	6	44	theme	ω7c-11	771:776	arg1	methyl					778:783	ω7c-11 methyl	771:783	ω7c-11 methyl	771:783	The predominant fatty acids were C18 : 1 ω7c, C18 : 1 ω7c-11 methyl and C16 : 0.
36260499	2	45	theme	aerobic	128:134	arg1	bacterium					182:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	5	46	theme	genus	698:702	arg1	Tabrizicola					704:714	the genus Tabrizicola	694:714	the genus Tabrizicola	694:714	Cellular components of J26T supported this strain as a member of the genus Tabrizicola.
36260499	5	47	theme	J26T	652:655	arg1	components					638:647	Cellular components	629:647	Cellular components of J26T	629:655	Cellular components of J26T supported this strain as a member of the genus Tabrizicola.
36260499	9	48	theme	novel	1164:1168	arg1	species					1170:1176	a novel species	1162:1176	a novel species	1162:1176	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	8	49	theme	DNA	948:950	arg1	%					976:976	64.2 mol%	968:976	64.2 mol%	968:976	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	8	49	theme	DNA	948:950	arg1	content					956:962	the DNA G+C content	944:962	the DNA G+C content	944:962	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	4	50	contain	had	507:509	arg1	J26T					502:505	strain J26T	495:505	strain J26T	495:505	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	4	50	contain	had	507:509	arg2	similarity					523:532	the highest similarity	511:532	the highest similarity	511:532	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	10	51	theme	name	1403:1406	arg1	sp					1433:1434	the name Tabrizicola rongguiensis sp	1399:1434	the name Tabrizicola rongguiensis sp	1399:1434	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	2	52	theme	rod-shaped	171:180	arg1	bacterium					182:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	3	53	theme	white	373:377	arg1	colonies					389:396	milky white irregular colonies	367:396	milky white irregular colonies on Reasoner's 2A agar medium	367:425	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36260499	2	54	from	sediment	232:239	arg1	Foshan					264:269	Foshan	264:269	Foshan	264:269	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	2	54	from	sediment	232:239	arg1	China					277:281	China	277:281	China	277:281	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	10	55	theme	properties	1272:1281	arg1	terms					1233:1237	terms	1233:1237	terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism,	1233:1331	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	10	56	theme	sulphur	1313:1319	arg1	metabolism					1321:1330	sulphur metabolism	1313:1330	sulphur metabolism	1313:1330	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	12	57	theme	1.2843T=KCTC	1487:1498	arg1	92112T					1500:1505	=GDMCC 1.2843T=KCTC 92112T	1480:1505	=GDMCC 1.2843T=KCTC 92112T	1480:1505	The type strain is J26T (=GDMCC 1.2843T=KCTC 92112T).
36260499	12	57	theme	1.2843T=KCTC	1487:1498	arg1	J26T					1474:1477	J26T	1474:1477	J26T (=GDMCC 1.2843T=KCTC 92112T)	1474:1506	The type strain is J26T (=GDMCC 1.2843T=KCTC 92112T).
36260499	2	58	attach	isolated	214:221	arg2	bacterium					182:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	2	58	attach	isolated	214:221	arg1	sediment					232:239	the sediment	228:239	the sediment of a river in Ronggui, Foshan city, China	228:281	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	8	59	theme	G+C	952:954	arg1	%					976:976	64.2 mol%	968:976	64.2 mol%	968:976	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	8	59	theme	G+C	952:954	arg1	content					956:962	the DNA G+C content	944:962	the DNA G+C content	944:962	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	2	60	theme	non-motile	156:165	arg1	bacterium					182:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium	105:190	A novel Gram-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterium, designated J26T, was isolated from the sediment of a river in Ronggui, Foshan city, China.
36260499	7	61	theme	Polar	798:802	arg1	lipids					804:809	Polar lipids	798:809	Polar lipids	798:809	Polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphorylethanolamine.
36260499	10	62	from	differences	1193:1203	arg1	terms					1233:1237	terms	1233:1237	terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism,	1233:1331	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	8	63	theme	64.2 mol	968:975	arg1	%					976:976	64.2 mol%	968:976	64.2 mol%	968:976	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	8	63	theme	64.2 mol	968:975	arg1	content					956:962	the DNA G+C content	944:962	the DNA G+C content	944:962	Ubiquinone Q-10 was the major respiratory quinone, and the DNA G+C content was 64.2 mol%.
36260499	4	64	theme	strain	495:500	arg1	J26T					502:505	strain J26T	495:505	strain J26T	495:505	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J26T had the highest similarity to Tabrizicola aquatica RCRI19T (97.1 %) and formed a distinct clade in the genus Tabrizicola.
36260499	10	65	theme	Tabrizicola	1408:1418	arg1	sp					1433:1434	the name Tabrizicola rongguiensis sp	1399:1434	the name Tabrizicola rongguiensis sp	1399:1434	Moreover, the differences between J26T and RCRI19T in terms of physiological and biochemical properties, such as carbon, nitrogen and sulphur metabolism, further supported that J26T represents a novel species, for which the name Tabrizicola rongguiensis sp.
36260499	9	66	theme	low	988:990	arg1	similarity					1015:1024	low 16S rRNA gene sequence similarity	988:1024	low 16S rRNA gene sequence similarity	988:1024	However, low 16S rRNA gene sequence similarity and average nucleotide identity (73.56 % for ANIb between strain J26T with RCRI19T) demonstrated that strain J26T should be assigned to a novel species.
36260499	3	67	theme	0 	314:315	arg1	%					316:316	%	316:316	%	316:316	Strain J26T grew optimally at 0 % (w/v) NaCl, pH 6.5-7.5, and 30 °C, and it formed milky white irregular colonies on Reasoner's 2A agar medium.
36256451	3	0	theme	strain	290:295	arg1	characteristics					266:280	The phylogenetic, chemotaxonomic and phenotypic characteristics	218:280	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain	218:295	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain were determined.
36256451	13	1	theme	VTCC	1284:1287	arg1	12236T					1289:1294	=KCTC 33932T= VTCC 12236T	1270:1294	=KCTC 33932T= VTCC 12236T	1270:1294	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	13	1	theme	VTCC	1284:1287	arg1	N4T					1265:1267	N4T	1265:1267	N4T (=KCTC 33932T= VTCC 12236T)	1265:1295	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	5	2	theme	Strain	397:402	arg1	N4T					404:406	Strain N4T	397:406	Strain N4T	397:406	Strain N4T grew at 20-37 °C (optimum, 30 °C), pH 6-10 (optimum, pH 7) and 0-5 % NaCl (optimum, 0 %).
36256451	2	3	from	soil	146:149	arg1	Vietnam					209:215	Vietnam	209:215	Vietnam	209:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	3	from	soil	146:149	arg1	province					199:206	Nghean province	192:206	Nghean province	192:206	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	4	theme	bacterial	101:109	arg1	strain					111:116	A novel bacterial strain	93:116	A novel bacterial strain	93:116	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	4	theme	bacterial	101:109	arg1	N4T					119:121	N4T	119:121	N4T	119:121	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	6	5	theme	=KCTC	722:726	arg1	3956T					728:732	=KCTC 3956T	722:732	=KCTC 3956T	722:732	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	5	theme	=KCTC	722:726	arg1	KSL-134T					712:719	Paenibacillus alkaliterrae KSL-134T	685:719	Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T)	685:733	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	7	6	theme	diamino	851:857	arg1	acid					828:831	meso-diaminopimelic acid	808:831	meso-diaminopimelic acid	808:831	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	7	6	theme	diamino	851:857	arg1	acid					859:862	the diagnostic diamino acid	836:862	the diagnostic diamino acid in the cell-wall peptidoglycan	836:893	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	7	7	from	acid	859:862	arg1	peptidoglycan					881:893	the cell-wall peptidoglycan	867:893	the cell-wall peptidoglycan	867:893	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	9	8	theme	major	935:939	arg1	MK-7					964:967	MK-7	964:967	MK-7	964:967	The major isoprenoid quinone was MK-7.
36256451	9	8	theme	major	935:939	arg1	quinone					952:958	The major isoprenoid quinone	931:958	The major isoprenoid quinone	931:958	The major isoprenoid quinone was MK-7.
36256451	2	9	theme	novel	95:99	arg1	strain					111:116	A novel bacterial strain	93:116	A novel bacterial strain	93:116	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	9	theme	novel	95:99	arg1	N4T					119:121	N4T	119:121	N4T	119:121	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	5	10	dep	optimum	426:432	arg1	30 °C					435:439	30 °C	435:439	30 °C	435:439	Strain N4T grew at 20-37 °C (optimum, 30 °C), pH 6-10 (optimum, pH 7) and 0-5 % NaCl (optimum, 0 %).
36256451	9	11	theme	isoprenoid	941:950	arg1	MK-7					964:967	MK-7	964:967	MK-7	964:967	The major isoprenoid quinone was MK-7.
36256451	9	11	theme	isoprenoid	941:950	arg1	quinone					952:958	The major isoprenoid quinone	931:958	The major isoprenoid quinone	931:958	The major isoprenoid quinone was MK-7.
36256451	11	12	theme	physiological	1065:1077	arg1	characteristics					1095:1109	phylogenetic, physiological and biochemical characteristics	1051:1109	phylogenetic, physiological and biochemical characteristics	1051:1109	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	5	13	dep	optimum	452:458	arg1	pH					461:462	pH 7	461:464	pH 7	461:464	Strain N4T grew at 20-37 °C (optimum, 30 °C), pH 6-10 (optimum, pH 7) and 0-5 % NaCl (optimum, 0 %).
36256451	6	14	theme	Paenibacillus	685:697	arg1	3956T					728:732	=KCTC 3956T	722:732	=KCTC 3956T	722:732	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	14	theme	Paenibacillus	685:697	arg1	KSL-134T					712:719	Paenibacillus alkaliterrae KSL-134T	685:719	Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T)	685:733	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	1	15	theme	rhizosphere	55:65	arg1	soil					67:70	the rhizosphere soil	51:70	the rhizosphere soil of Arachis hypogaea	51:90	nov., isolated from the rhizosphere soil of Arachis hypogaea.
36256451	6	16	theme	Paenibacillus	598:610	arg1	genus					592:596	the genus Paenibacillus	588:610	the genus Paenibacillus	588:610	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	17	with	related	628:634	arg1	similarity					769:778	96.3 and 96.5% gene sequence similarity	740:778	96.3 and 96.5% gene sequence similarity	740:778	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	18	theme	sequence	760:767	arg1	similarity					769:778	96.3 and 96.5% gene sequence similarity	740:778	96.3 and 96.5% gene sequence similarity	740:778	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	7	19	theme	diagnostic	840:849	arg1	acid					828:831	meso-diaminopimelic acid	808:831	meso-diaminopimelic acid	808:831	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	7	19	theme	diagnostic	840:849	arg1	acid					859:862	the diagnostic diamino acid	836:862	the diagnostic diamino acid in the cell-wall peptidoglycan	836:893	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	2	20	from	field	183:187	arg1	Vietnam					209:215	Vietnam	209:215	Vietnam	209:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	20	from	field	183:187	arg1	province					199:206	Nghean province	192:206	Nghean province	192:206	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	11	21	theme	vietnamensis	1211:1222	arg1	sp					1224:1225	the name Paenibacillus vietnamensis sp	1188:1225	the name Paenibacillus vietnamensis sp	1188:1225	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	6	22	theme	gene	755:758	arg1	similarity					769:778	96.3 and 96.5% gene sequence similarity	740:778	96.3 and 96.5% gene sequence similarity	740:778	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	7	23	contain	contained	798:806	arg2	acid					859:862	the diagnostic diamino acid	836:862	the diagnostic diamino acid in the cell-wall peptidoglycan	836:893	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	7	23	contain	contained	798:806	arg2	acid					828:831	meso-diaminopimelic acid	808:831	meso-diaminopimelic acid	808:831	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	7	23	contain	contained	798:806	arg1	It					795:796	It	795:796	It	795:796	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	2	24	from	province	199:206	arg1	soil					146:149	the soil	142:149	the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam	142:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	0	25	theme	vietnamensis	14:25	arg1	sp					27:28	Paenibacillus vietnamensis sp	0:28	Paenibacillus vietnamensis sp.	0:29	Paenibacillus vietnamensis sp.
36256451	10	26	theme	iso-C16 	990:997	arg1	acids					1035:1039	the dominant cellular fatty acids	1007:1039	the dominant cellular fatty acids	1007:1039	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	10	26	theme	iso-C16 	990:997	arg1	Anteiso-C15 					970:981	Anteiso-C15 	970:981	Anteiso-C15 : 0 and iso-C16 : 0	970:1000	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	8	27	theme	52.9 mol	920:927	arg1	content					908:914	The DNA G+C content	896:914	The DNA G+C content	896:914	The DNA G+C content was 52.9 mol%.
36256451	8	27	theme	52.9 mol	920:927	arg1	%					928:928	52.9 mol%	920:928	52.9 mol%	920:928	The DNA G+C content was 52.9 mol%.
36256451	1	28	theme	Arachis	75:81	arg1	hypogaea					83:90	Arachis hypogaea	75:90	Arachis hypogaea	75:90	nov., isolated from the rhizosphere soil of Arachis hypogaea.
36256451	10	29	theme	 0	983:984	arg1	acids					1035:1039	the dominant cellular fatty acids	1007:1039	the dominant cellular fatty acids	1007:1039	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	10	29	theme	 0	983:984	arg1	Anteiso-C15 					970:981	Anteiso-C15 	970:981	Anteiso-C15 : 0 and iso-C16 : 0	970:1000	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	0	30	theme	Paenibacillus	0:12	arg1	sp					27:28	Paenibacillus vietnamensis sp	0:28	Paenibacillus vietnamensis sp.	0:29	Paenibacillus vietnamensis sp.
36256451	6	31	theme	=KCTC	668:672	arg1	3951T					674:678	=KCTC 3951T	668:678	=KCTC 3951T	668:678	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	31	theme	=KCTC	668:672	arg1	B519T					661:665	Paenibacillus harenae B519T	639:665	Paenibacillus harenae B519T (=KCTC 3951T)	639:679	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	1	32	theme	hypogaea	83:90	arg1	soil					67:70	the rhizosphere soil	51:70	the rhizosphere soil of Arachis hypogaea	51:90	nov., isolated from the rhizosphere soil of Arachis hypogaea.
36256451	11	33	theme	genus	1157:1161	arg1	species					1142:1148	a novel species	1134:1148	a novel species	1134:1148	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	8	34	theme	G+C	904:906	arg1	content					908:914	The DNA G+C content	896:914	The DNA G+C content	896:914	The DNA G+C content was 52.9 mol%.
36256451	8	34	theme	G+C	904:906	arg1	%					928:928	52.9 mol%	920:928	52.9 mol%	920:928	The DNA G+C content was 52.9 mol%.
36256451	3	35	theme	chemotaxonomic	236:249	arg1	characteristics					266:280	The phylogenetic, chemotaxonomic and phenotypic characteristics	218:280	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain	218:295	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain were determined.
36256451	6	36	theme	Paenibacillus	639:651	arg1	3951T					674:678	=KCTC 3951T	668:678	=KCTC 3951T	668:678	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	36	theme	Paenibacillus	639:651	arg1	B519T					661:665	Paenibacillus harenae B519T	639:665	Paenibacillus harenae B519T (=KCTC 3951T)	639:679	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	37	theme	alkaliterrae	699:710	arg1	3956T					728:732	=KCTC 3956T	722:732	=KCTC 3956T	722:732	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	37	theme	alkaliterrae	699:710	arg1	KSL-134T					712:719	Paenibacillus alkaliterrae KSL-134T	685:719	Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T)	685:733	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	8	38	theme	DNA	900:902	arg1	content					908:914	The DNA G+C content	896:914	The DNA G+C content	896:914	The DNA G+C content was 52.9 mol%.
36256451	8	38	theme	DNA	900:902	arg1	%					928:928	52.9 mol%	920:928	52.9 mol%	920:928	The DNA G+C content was 52.9 mol%.
36256451	11	39	theme	name	1192:1195	arg1	sp					1224:1225	the name Paenibacillus vietnamensis sp	1188:1225	the name Paenibacillus vietnamensis sp	1188:1225	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	5	40	theme	%	475:475	arg1	 NaCl					476:480	0-5 % NaCl	471:480	0-5 % NaCl (optimum, 0 %)	471:495	Strain N4T grew at 20-37 °C (optimum, 30 °C), pH 6-10 (optimum, pH 7) and 0-5 % NaCl (optimum, 0 %).
36256451	6	41	theme	strain	565:570	arg1	N4T					572:574	strain N4T	565:574	strain N4T	565:574	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	5	42	dep	 NaCl	476:480	arg1	optimum					483:489	optimum	483:489	optimum	483:489	Strain N4T grew at 20-37 °C (optimum, 30 °C), pH 6-10 (optimum, pH 7) and 0-5 % NaCl (optimum, 0 %).
36256451	5	42	dep	 NaCl	476:480	arg1	%					494:494	0 %	492:494	0 %	492:494	Strain N4T grew at 20-37 °C (optimum, 30 °C), pH 6-10 (optimum, pH 7) and 0-5 % NaCl (optimum, 0 %).
36256451	3	43	theme	phylogenetic	222:233	arg1	characteristics					266:280	The phylogenetic, chemotaxonomic and phenotypic characteristics	218:280	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain	218:295	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain were determined.
36256451	10	44	theme	fatty	1029:1033	arg1	acids					1035:1039	the dominant cellular fatty acids	1007:1039	the dominant cellular fatty acids	1007:1039	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	10	44	theme	fatty	1029:1033	arg1	Anteiso-C15 					970:981	Anteiso-C15 	970:981	Anteiso-C15 : 0 and iso-C16 : 0	970:1000	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	2	45	theme	Nghean	192:197	arg1	Vietnam					209:215	Vietnam	209:215	Vietnam	209:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	45	theme	Nghean	192:197	arg1	province					199:206	Nghean province	192:206	Nghean province	192:206	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	13	46	theme	type	1250:1253	arg1	N4T					1265:1267	N4T	1265:1267	N4T (=KCTC 33932T= VTCC 12236T)	1265:1295	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	13	46	theme	type	1250:1253	arg1	strain					1255:1260	The type strain	1246:1260	The type strain	1246:1260	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	10	47	theme	cellular	1020:1027	arg1	acids					1035:1039	the dominant cellular fatty acids	1007:1039	the dominant cellular fatty acids	1007:1039	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	10	47	theme	cellular	1020:1027	arg1	Anteiso-C15 					970:981	Anteiso-C15 	970:981	Anteiso-C15 : 0 and iso-C16 : 0	970:1000	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	5	48	theme	0-5 	471:474	arg1	%					475:475	%	475:475	%	475:475	Strain N4T grew at 20-37 °C (optimum, 30 °C), pH 6-10 (optimum, pH 7) and 0-5 % NaCl (optimum, 0 %).
36256451	10	49	theme	dominant	1011:1018	arg1	acids					1035:1039	the dominant cellular fatty acids	1007:1039	the dominant cellular fatty acids	1007:1039	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	10	49	theme	dominant	1011:1018	arg1	Anteiso-C15 					970:981	Anteiso-C15 	970:981	Anteiso-C15 : 0 and iso-C16 : 0	970:1000	Anteiso-C15 : 0 and iso-C16 : 0 were the dominant cellular fatty acids.
36256451	11	50	theme	novel	1136:1140	arg1	species					1142:1148	a novel species	1134:1148	a novel species	1134:1148	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	2	51	theme	hypogaea	174:181	arg1	field					183:187	a groundnut Arachis hypogaea field	154:187	a groundnut Arachis hypogaea field in Nghean province, Vietnam	154:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	52	theme	field	183:187	arg1	soil					146:149	the soil	142:149	the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam	142:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	4	53	theme	N4T	330:332	arg1	Cells					314:318	Cells	314:318	Cells of strain N4T	314:332	Cells of strain N4T were Gram-negative, aerobic, endospore-forming and rod-shaped.
36256451	2	54	attach	isolated	128:135	arg1	soil					146:149	the soil	142:149	the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam	142:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	54	attach	isolated	128:135	arg2	N4T					119:121	N4T	119:121	N4T	119:121	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	2	54	attach	isolated	128:135	arg2	strain					111:116	A novel bacterial strain	93:116	A novel bacterial strain	93:116	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	11	55	theme	strain	1112:1117	arg1	N4T					1119:1121	strain N4T	1112:1121	strain N4T	1112:1121	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	7	56	theme	meso-diaminopimelic	808:826	arg1	acid					828:831	meso-diaminopimelic acid	808:831	meso-diaminopimelic acid	808:831	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	7	56	theme	meso-diaminopimelic	808:826	arg1	acid					859:862	the diagnostic diamino acid	836:862	the diagnostic diamino acid in the cell-wall peptidoglycan	836:893	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	3	57	theme	phenotypic	255:264	arg1	characteristics					266:280	The phylogenetic, chemotaxonomic and phenotypic characteristics	218:280	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain	218:295	The phylogenetic, chemotaxonomic and phenotypic characteristics of this strain were determined.
36256451	2	58	theme	Arachis	166:172	arg1	field					183:187	a groundnut Arachis hypogaea field	154:187	a groundnut Arachis hypogaea field in Nghean province, Vietnam	154:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	4	59	theme	strain	323:328	arg1	N4T					330:332	strain N4T	323:332	strain N4T	323:332	Cells of strain N4T were Gram-negative, aerobic, endospore-forming and rod-shaped.
36256451	11	60	theme	phylogenetic	1051:1062	arg1	characteristics					1095:1109	phylogenetic, physiological and biochemical characteristics	1051:1109	phylogenetic, physiological and biochemical characteristics	1051:1109	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	6	61	theme	harenae	653:659	arg1	3951T					674:678	=KCTC 3951T	668:678	=KCTC 3951T	668:678	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	61	theme	harenae	653:659	arg1	B519T					661:665	Paenibacillus harenae B519T	639:665	Paenibacillus harenae B519T (=KCTC 3951T)	639:679	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	11	62	theme	Paenibacillus	1163:1175	arg1	genus					1157:1161	the genus Paenibacillus	1153:1175	the genus Paenibacillus	1153:1175	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	2	63	theme	groundnut	156:164	arg1	field					183:187	a groundnut Arachis hypogaea field	154:187	a groundnut Arachis hypogaea field in Nghean province, Vietnam	154:215	A novel bacterial strain, N4T, was isolated from the soil of a groundnut Arachis hypogaea field in Nghean province, Vietnam.
36256451	11	64	theme	Paenibacillus	1197:1209	arg1	sp					1224:1225	the name Paenibacillus vietnamensis sp	1188:1225	the name Paenibacillus vietnamensis sp	1188:1225	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	7	65	theme	cell-wall	871:879	arg1	peptidoglycan					881:893	the cell-wall peptidoglycan	867:893	the cell-wall peptidoglycan	867:893	It contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
36256451	13	66	theme	33932T=	1276:1282	arg1	12236T					1289:1294	=KCTC 33932T= VTCC 12236T	1270:1294	=KCTC 33932T= VTCC 12236T	1270:1294	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	13	66	theme	33932T=	1276:1282	arg1	N4T					1265:1267	N4T	1265:1267	N4T (=KCTC 33932T= VTCC 12236T)	1265:1295	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	6	67	theme	gene	538:541	arg1	sequences					543:551	16S rRNA gene sequences	529:551	16S rRNA gene sequences	529:551	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	68	dep	%	753:753	arg1	96.3					740:743	96.3	740:743	96.3	740:743	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	68	dep	%	753:753	arg1	96.5					749:752	96.5	749:752	96.5	749:752	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	13	69	theme	=KCTC	1270:1274	arg1	12236T					1289:1294	=KCTC 33932T= VTCC 12236T	1270:1294	=KCTC 33932T= VTCC 12236T	1270:1294	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	13	69	theme	=KCTC	1270:1274	arg1	N4T					1265:1267	N4T	1265:1267	N4T (=KCTC 33932T= VTCC 12236T)	1265:1295	The type strain is N4T (=KCTC 33932T= VTCC 12236T).
36256451	6	70	theme	rRNA	533:536	arg1	sequences					543:551	16S rRNA gene sequences	529:551	16S rRNA gene sequences	529:551	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	71	dep	similarity	769:778	arg1	%					753:753	%	753:753	%	753:753	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	11	72	theme	biochemical	1083:1093	arg1	characteristics					1095:1109	phylogenetic, physiological and biochemical characteristics	1051:1109	phylogenetic, physiological and biochemical characteristics	1051:1109	Based on phylogenetic, physiological and biochemical characteristics, strain N4T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus vietnamensis sp.
36256451	6	73	theme	Phylogenetic	498:509	arg1	analysis					511:518	Phylogenetic analysis	498:518	Phylogenetic analysis based on 16S rRNA gene sequences	498:551	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
36256451	6	74	theme	16S	529:531	arg1	sequences					543:551	16S rRNA gene sequences	529:551	16S rRNA gene sequences	529:551	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain N4T belonged to the genus Paenibacillus and was closely related to Paenibacillus harenae B519T (=KCTC 3951T) and Paenibacillus alkaliterrae KSL-134T (=KCTC 3956T) with 96.3 and 96.5% gene sequence similarity, respectively.
35763968	1	0	theme	development	239:249	arg1	source					220:225	a valuable and inexpensive source	193:225	a valuable and inexpensive source of new drug development	193:249	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	1	0	theme	development	239:249	arg1	Plants					170:175	Plants	170:175	Plants	170:175	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	12	1	theme	protein	1944:1950	arg1	target					1952:1957	protein target	1944:1957	protein target using computational tools	1944:1983	Similarly, the extract also revealed promising anti-inflammatory activities in vivo while exhibiting variable Pharmacokinetics and binding affinities towards protein target using computational tools.
35763968	3	2	theme	alkaloids	524:532	arg1	presence					512:519	The presence	508:519	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda	508:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	11	3	theme	significant	1709:1719	arg1	antioxidants					1721:1732	significant antioxidants	1709:1732	significant antioxidants	1709:1732	In conclusion, the leaf extract exhibited significant antioxidants and antibacterial activities using in vitro assays.
35763968	0	4	theme	Carrageenan	99:109	arg1	models					132:137	Carrageenan and Formalin-induced models	99:137	Carrageenan and Formalin-induced models	99:137	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	11	5	theme	in	1769:1770	arg1	assays					1778:1783	in vitro assays	1769:1783	in vitro assays	1769:1783	In conclusion, the leaf extract exhibited significant antioxidants and antibacterial activities using in vitro assays.
35763968	0	6	theme	Formalin-induced	115:130	arg1	models					132:137	Carrageenan and Formalin-induced models	99:137	Carrageenan and Formalin-induced models	99:137	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	7	7	located	found	1211:1215	arg2	acid					1202:1205	gallic acid	1195:1205	gallic acid	1195:1205	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	7	7	located	found	1211:1215	arg1	concentration					1232:1244	the highest concentration	1220:1244	the highest concentration with a 45.42% composition	1220:1270	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	3	8	theme	tannins	545:551	arg1	presence					512:519	The presence	508:519	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda	508:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	8	9	theme	lowest	1359:1364	arg1	activity					1366:1373	the lowest activity	1355:1373	the lowest activity against Listeria	1355:1390	The leaf extract showed the highest antibacterial activity against E. coli, while the lowest activity against Listeria was observed.
35763968	7	10	with	concentration	1232:1244	arg1	composition					1260:1270	a 45.42% composition	1251:1270	a 45.42% composition	1251:1270	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	3	11	theme	saponins	535:542	arg1	presence					512:519	The presence	508:519	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda	508:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	5	12	theme	Carrageenan	852:862	arg1	models					903:908	Carrageenan and Formalin-induced inflammatory mice models	852:908	Carrageenan and Formalin-induced inflammatory mice models	852:908	Similarly, the anti-inflammatory potential of the leaf extract was assessed in Carrageenan and Formalin-induced inflammatory mice models.
35763968	1	13	used	used	299:302	arg2	compounds					275:283	plant compounds	269:283	plant compounds	269:283	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	1	13	used	used	299:302	arg2	variety					258:264	a variety	256:264	a variety of plant compounds	256:283	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	3	14	from	presence	512:519	arg1	extract					602:608	the leaf extract	593:608	the leaf extract of J. adhatoda	593:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	0	15	theme	oxidative	152:160	arg1	stress					162:167	oxidative stress	152:167	oxidative stress	152:167	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	3	16	theme	phenols	568:574	arg1	presence					512:519	The presence	508:519	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda	508:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	5	17	theme	Formalin-induced	868:883	arg1	models					903:908	Carrageenan and Formalin-induced inflammatory mice models	852:908	Carrageenan and Formalin-induced inflammatory mice models	852:908	Similarly, the anti-inflammatory potential of the leaf extract was assessed in Carrageenan and Formalin-induced inflammatory mice models.
35763968	2	18	theme	Justicia	459:466	arg1	extract					485:491	Justicia adhatoda L. leaf extract	459:491	Justicia adhatoda L. leaf extract	459:491	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	7	19	theme	45.42	1253:1257	arg1	%					1258:1258	%	1258:1258	%	1258:1258	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	3	20	theme	phytosterols	554:565	arg1	presence					512:519	The presence	508:519	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda	508:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	5	21	theme	extract	828:834	arg1	potential					806:814	the anti-inflammatory potential	784:814	the anti-inflammatory potential of the leaf extract	784:834	Similarly, the anti-inflammatory potential of the leaf extract was assessed in Carrageenan and Formalin-induced inflammatory mice models.
35763968	1	22	theme	clinical	307:314	arg1	trials					316:321	clinical trials	307:321	clinical trials	307:321	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	2	23	dep	evaluate	401:408	arg1	Acanthaceae					494:504	Acanthaceae	494:504	Acanthaceae	494:504	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	6	24	theme	extract	950:956	arg1	analysis					929:936	The phytochemical analysis	911:936	The phytochemical analysis of the leaf extract	911:956	The phytochemical analysis of the leaf extract indicated a positive test for alkaloids, saponins, tannins, phytosterols, phenols, proteins, and amino acids, while the negative test for carbohydrates, and glycosides, flavonoids, and diterpenes.
35763968	2	25	theme	extract	485:491	arg1	effects					448:454	the anti-inflammatory and antioxidant effects	410:454	the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract	410:491	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	4	26	theme	compounds	709:717	arg1	identification					681:694	the identification	677:694	the identification of different compounds in the leaf extract	677:737	While the identification of different compounds in the leaf extract was carried out by HPLC analysis.
35763968	2	27	theme	L.	477:478	arg1	extract					485:491	Justicia adhatoda L. leaf extract	459:491	Justicia adhatoda L. leaf extract	459:491	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	0	28	from	extract	50:56	arg1	models					132:137	Carrageenan and Formalin-induced models	99:137	Carrageenan and Formalin-induced models	99:137	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	11	29	dep	in	1769:1770	arg1	vitro					1772:1776	vitro	1772:1776	vitro	1772:1776	In conclusion, the leaf extract exhibited significant antioxidants and antibacterial activities using in vitro assays.
35763968	12	30	theme	promising	1823:1831	arg1	activities					1851:1860	promising anti-inflammatory activities	1823:1860	promising anti-inflammatory activities	1823:1860	Similarly, the extract also revealed promising anti-inflammatory activities in vivo while exhibiting variable Pharmacokinetics and binding affinities towards protein target using computational tools.
35763968	3	31	attach	presence	512:519	arg2	phenols					568:574	phenols	568:574	phenols	568:574	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	3	31	attach	presence	512:519	arg2	saponins					535:542	saponins	535:542	saponins	535:542	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	3	31	attach	presence	512:519	arg2	tannins					545:551	tannins	545:551	tannins	545:551	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	3	31	attach	presence	512:519	arg2	alkaloids					524:532	alkaloids	524:532	alkaloids	524:532	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	3	31	attach	presence	512:519	arg2	phytosterols					554:565	phytosterols	554:565	phytosterols	554:565	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	3	31	attach	presence	512:519	arg1	extract					602:608	the leaf extract	593:608	the leaf extract of J. adhatoda	593:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	3	31	attach	presence	512:519	arg2	proteins					581:588	proteins	581:588	proteins	581:588	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	8	32	theme	leaf	1277:1280	arg1	extract					1282:1288	The leaf extract	1273:1288	The leaf extract	1273:1288	The leaf extract showed the highest antibacterial activity against E. coli, while the lowest activity against Listeria was observed.
35763968	0	33	theme	Anti-inflammatory	0:16	arg1	potential					32:40	Anti-inflammatory and analgesic potential	0:40	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.	0:168	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	5	34	theme	mice	898:901	arg1	models					903:908	Carrageenan and Formalin-induced inflammatory mice models	852:908	Carrageenan and Formalin-induced inflammatory mice models	852:908	Similarly, the anti-inflammatory potential of the leaf extract was assessed in Carrageenan and Formalin-induced inflammatory mice models.
35763968	2	35	theme	work	372:375	arg1	goal					359:362	The goal	355:362	The goal of this work	355:375	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	0	36	theme	analgesic	22:30	arg1	potential					32:40	Anti-inflammatory and analgesic potential	0:40	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.	0:168	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	8	37	theme	highest	1301:1307	arg1	activity					1323:1330	the highest antibacterial activity	1297:1330	the highest antibacterial activity	1297:1330	The leaf extract showed the highest antibacterial activity against E. coli, while the lowest activity against Listeria was observed.
35763968	5	38	theme	anti-inflammatory	788:804	arg1	potential					806:814	the anti-inflammatory potential	784:814	the anti-inflammatory potential of the leaf extract	784:834	Similarly, the anti-inflammatory potential of the leaf extract was assessed in Carrageenan and Formalin-induced inflammatory mice models.
35763968	1	39	theme	compounds	275:283	arg1	variety					258:264	a variety	256:264	a variety of plant compounds	256:283	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	1	39	theme	compounds	275:283	arg1	compounds					275:283	plant compounds	269:283	plant compounds	269:283	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	0	40	theme	extract	50:56	arg1	potential					32:40	Anti-inflammatory and analgesic potential	0:40	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.	0:168	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	1	41	theme	inexpensive	208:218	arg1	source					220:225	a valuable and inexpensive source	193:225	a valuable and inexpensive source of new drug development	193:249	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	1	41	theme	inexpensive	208:218	arg1	Plants					170:175	Plants	170:175	Plants	170:175	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	3	42	theme	leaf	597:600	arg1	extract					602:608	the leaf extract	593:608	the leaf extract of J. adhatoda	593:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	7	43	theme	detected	1175:1182	arg1	compounds					1184:1192	the detected compounds	1171:1192	the detected compounds	1171:1192	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	1	44	theme	drug	234:237	arg1	development					239:249	new drug development	230:249	new drug development	230:249	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	3	45	theme	adhatoda	616:623	arg1	extract					602:608	the leaf extract	593:608	the leaf extract of J. adhatoda	593:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	12	46	theme	computational	1965:1977	arg1	tools					1979:1983	computational tools	1965:1983	computational tools	1965:1983	Similarly, the extract also revealed promising anti-inflammatory activities in vivo while exhibiting variable Pharmacokinetics and binding affinities towards protein target using computational tools.
35763968	4	47	dep	carried	743:749	arg1	While					671:675	While	671:675	While	671:675	While the identification of different compounds in the leaf extract was carried out by HPLC analysis.
35763968	3	48	theme	phytochemical	646:658	arg1	screening					660:668	phytochemical screening	646:668	phytochemical screening	646:668	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	6	49	theme	negative	1078:1085	arg1	test					1087:1090	the negative test	1074:1090	the negative test for carbohydrates, and glycosides, flavonoids, and diterpenes	1074:1152	The phytochemical analysis of the leaf extract indicated a positive test for alkaloids, saponins, tannins, phytosterols, phenols, proteins, and amino acids, while the negative test for carbohydrates, and glycosides, flavonoids, and diterpenes.
35763968	11	50	theme	antibacterial	1738:1750	arg1	activities					1752:1761	antibacterial activities	1738:1761	antibacterial activities	1738:1761	In conclusion, the leaf extract exhibited significant antioxidants and antibacterial activities using in vitro assays.
35763968	8	51	theme	antibacterial	1309:1321	arg1	activity					1323:1330	the highest antibacterial activity	1297:1330	the highest antibacterial activity	1297:1330	The leaf extract showed the highest antibacterial activity against E. coli, while the lowest activity against Listeria was observed.
35763968	6	52	theme	phytochemical	915:927	arg1	analysis					929:936	The phytochemical analysis	911:936	The phytochemical analysis of the leaf extract	911:956	The phytochemical analysis of the leaf extract indicated a positive test for alkaloids, saponins, tannins, phytosterols, phenols, proteins, and amino acids, while the negative test for carbohydrates, and glycosides, flavonoids, and diterpenes.
35763968	1	53	theme	ailments	345:352	arg1	ailments					345:352	ailments	345:352	ailments	345:352	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	1	53	theme	ailments	345:352	arg1	variety					334:340	a variety	332:340	a variety of ailments	332:352	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	1	54	theme	valuable	195:202	arg1	source					220:225	a valuable and inexpensive source	193:225	a valuable and inexpensive source of new drug development	193:249	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	1	54	theme	valuable	195:202	arg1	Plants					170:175	Plants	170:175	Plants	170:175	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	12	55	theme	binding	1917:1923	arg1	affinities					1925:1934	binding affinities	1917:1934	binding affinities	1917:1934	Similarly, the extract also revealed promising anti-inflammatory activities in vivo while exhibiting variable Pharmacokinetics and binding affinities towards protein target using computational tools.
35763968	9	56	theme	leaf	1410:1413	arg1	extract					1415:1421	The leaf extract	1406:1421	The leaf extract of J. adhatoda	1406:1436	The leaf extract of J. adhatoda revealed promising anti-inflammatory, analgesic, and antioxidants activities both in vitro and in vivo.
35763968	9	57	dep	anti-inflammatory	1457:1473	arg1	activities					1504:1513	activities	1504:1513	activities	1504:1513	The leaf extract of J. adhatoda revealed promising anti-inflammatory, analgesic, and antioxidants activities both in vitro and in vivo.
35763968	0	58	theme	adhatoda	70:77	arg1	extract					50:56	leaf extract	45:56	leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models	45:137	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	5	59	theme	leaf	823:826	arg1	extract					828:834	the leaf extract	819:834	the leaf extract	819:834	Similarly, the anti-inflammatory potential of the leaf extract was assessed in Carrageenan and Formalin-induced inflammatory mice models.
35763968	6	60	theme	positive	970:977	arg1	test					979:982	a positive test	968:982	a positive test for alkaloids, saponins, tannins, phytosterols, phenols, proteins, and amino acids	968:1065	The phytochemical analysis of the leaf extract indicated a positive test for alkaloids, saponins, tannins, phytosterols, phenols, proteins, and amino acids, while the negative test for carbohydrates, and glycosides, flavonoids, and diterpenes.
35763968	7	61	theme	%	1258:1258	arg1	composition					1260:1270	a 45.42% composition	1251:1270	a 45.42% composition	1251:1270	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	9	62	theme	adhatoda	1429:1436	arg1	extract					1415:1421	The leaf extract	1406:1421	The leaf extract of J. adhatoda	1406:1436	The leaf extract of J. adhatoda revealed promising anti-inflammatory, analgesic, and antioxidants activities both in vitro and in vivo.
35763968	2	63	theme	antioxidant	436:446	arg1	effects					448:454	the anti-inflammatory and antioxidant effects	410:454	the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract	410:491	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	12	64	theme	variable	1887:1894	arg1	Pharmacokinetics					1896:1911	variable Pharmacokinetics	1887:1911	variable Pharmacokinetics	1887:1911	Similarly, the extract also revealed promising anti-inflammatory activities in vivo while exhibiting variable Pharmacokinetics and binding affinities towards protein target using computational tools.
35763968	6	65	theme	leaf	945:948	arg1	extract					950:956	the leaf extract	941:956	the leaf extract	941:956	The phytochemical analysis of the leaf extract indicated a positive test for alkaloids, saponins, tannins, phytosterols, phenols, proteins, and amino acids, while the negative test for carbohydrates, and glycosides, flavonoids, and diterpenes.
35763968	2	66	theme	leaf	480:483	arg1	extract					485:491	Justicia adhatoda L. leaf extract	459:491	Justicia adhatoda L. leaf extract	459:491	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	4	67	theme	different	699:707	arg1	compounds					709:717	different compounds	699:717	different compounds	699:717	While the identification of different compounds in the leaf extract was carried out by HPLC analysis.
35763968	11	68	theme	leaf	1686:1689	arg1	extract					1691:1697	the leaf extract	1682:1697	the leaf extract	1682:1697	In conclusion, the leaf extract exhibited significant antioxidants and antibacterial activities using in vitro assays.
35763968	12	69	theme	anti-inflammatory	1833:1849	arg1	activities					1851:1860	promising anti-inflammatory activities	1823:1860	promising anti-inflammatory activities	1823:1860	Similarly, the extract also revealed promising anti-inflammatory activities in vivo while exhibiting variable Pharmacokinetics and binding affinities towards protein target using computational tools.
35763968	2	70	theme	adhatoda	468:475	arg1	extract					485:491	Justicia adhatoda L. leaf extract	459:491	Justicia adhatoda L. leaf extract	459:491	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	5	71	dep	Carrageenan	852:862	arg1	inflammatory					885:896	inflammatory	885:896	inflammatory	885:896	Similarly, the anti-inflammatory potential of the leaf extract was assessed in Carrageenan and Formalin-induced inflammatory mice models.
35763968	10	72	theme	detected	1557:1564	arg1	compounds					1566:1574	the detected compounds	1553:1574	the detected compounds	1553:1574	Similarly, the detected compounds portrayed variable pharmacokinetic as well as binding affinities with the target proteins.
35763968	4	73	from	identification	681:694	arg1	extract					731:737	the leaf extract	722:737	the leaf extract	722:737	While the identification of different compounds in the leaf extract was carried out by HPLC analysis.
35763968	7	74	theme	highest	1224:1230	arg1	concentration					1232:1244	the highest concentration	1220:1244	the highest concentration with a 45.42% composition	1220:1270	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	0	75	dep	adhatoda	70:77	arg1	L					79:79	L	79:79	Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models	61:137	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	3	76	theme	proteins	581:588	arg1	presence					512:519	The presence	508:519	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda	508:623	The presence of alkaloids, saponins, tannins, phytosterols, phenols, and proteins in the leaf extract of J. adhatoda was determined using phytochemical screening.
35763968	0	77	theme	leaf	45:48	arg1	extract					50:56	leaf extract	45:56	leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models	45:137	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	1	78	theme	plant	269:273	arg1	compounds					275:283	plant compounds	269:283	plant compounds	269:283	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
35763968	2	79	theme	anti-inflammatory	414:430	arg1	effects					448:454	the anti-inflammatory and antioxidant effects	410:454	the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract	410:491	The goal of this work was to characterize and evaluate the anti-inflammatory and antioxidant effects of Justicia adhatoda L. leaf extract (Acanthaceae).
35763968	7	80	theme	gallic	1195:1200	arg1	acid					1202:1205	gallic acid	1195:1205	gallic acid	1195:1205	Moreover, among the detected compounds, gallic acid was found in the highest concentration with a 45.42% composition.
35763968	4	81	theme	HPLC	758:761	arg1	analysis					763:770	HPLC analysis	758:770	HPLC analysis	758:770	While the identification of different compounds in the leaf extract was carried out by HPLC analysis.
35763968	10	82	theme	binding	1622:1628	arg1	affinities					1630:1639	binding affinities	1622:1639	variable pharmacokinetic as well as binding affinities	1586:1639	Similarly, the detected compounds portrayed variable pharmacokinetic as well as binding affinities with the target proteins.
35763968	6	83	theme	amino	1055:1059	arg1	acids					1061:1065	amino acids	1055:1065	amino acids	1055:1065	The phytochemical analysis of the leaf extract indicated a positive test for alkaloids, saponins, tannins, phytosterols, phenols, proteins, and amino acids, while the negative test for carbohydrates, and glycosides, flavonoids, and diterpenes.
35763968	0	84	from	models	132:137	arg1	extract					50:56	leaf extract	45:56	leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models	45:137	Anti-inflammatory and analgesic potential of leaf extract of Justicia adhatoda L. (Acanthaceae) in Carrageenan and Formalin-induced models by targeting oxidative stress.
35763968	4	85	theme	leaf	726:729	arg1	extract					731:737	the leaf extract	722:737	the leaf extract	722:737	While the identification of different compounds in the leaf extract was carried out by HPLC analysis.
35763968	10	86	theme	target	1650:1655	arg1	proteins					1657:1664	the target proteins	1646:1664	the target proteins	1646:1664	Similarly, the detected compounds portrayed variable pharmacokinetic as well as binding affinities with the target proteins.
35763968	1	87	theme	new	230:232	arg1	development					239:249	new drug development	230:249	new drug development	230:249	Plants are regarded as a valuable and inexpensive source of new drug development, and a variety of plant compounds are now being used in clinical trials to treat a variety of ailments.
36688924	3	0	theme	inflammasome	719:730	arg1	activation					732:741	NLRP3 inflammasome activation	713:741	NLRP3 inflammasome activation	713:741	The findings showed that both PIC and HPSB attenuated inflammation by inhibiting the TNF-α/NF-κB/MLC pathway and reducing NLRP3 inflammasome activation.
36688924	2	1	theme	3'-hydroxypterostilbene	366:388	arg1	effect					334:339	the effect	330:339	the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice	330:451	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	6	2	theme	representative	1043:1056	arg1	Lactobacillus					1107:1119	Lactobacillus	1107:1119	Lactobacillus	1107:1119	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	2	theme	representative	1043:1056	arg1	species					1068:1074	representative probiotic species	1043:1074	representative probiotic species	1043:1074	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	2	theme	representative	1043:1056	arg1	Akkermansiaceae					1087:1101	Akkermansiaceae	1087:1101	Akkermansiaceae	1087:1101	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	0	3	theme	Epithelial	103:112	arg1	Integrity					114:122	Intestinal Epithelial Integrity	92:122	Intestinal Epithelial Integrity	92:122	Piceatannol and 3'-Hydroxypterostilbene Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
36688924	5	4	theme	Bax/Bcl-2	932:940	arg1	activation					956:965	Bax/Bcl-2 and caspase-3 activation	932:965	activation	956:965	The results may be attributed to the effect of PIC on reducing cell apoptosis-associated protein expression, including Bax/Bcl-2 and caspase-3 activation.
36688924	2	5	theme	gut	532:534	arg1	dysfunction					544:554	gut barrier dysfunction	532:554	gut barrier dysfunction	532:554	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	7	6	from	biomarker	1341:1349	arg1	group					1362:1366	the PIC group	1354:1366	the PIC group	1354:1366	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	2	7	from	effect	334:339	arg1	colitis					437:443	dextran sulfate sodium (DSS)-induced colitis	400:443	dextran sulfate sodium (DSS)-induced colitis in mice	400:451	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	7	8	theme	butyrate-producing	1292:1309	arg1	biomarker					1341:1349	a biomarker	1339:1349	a biomarker in the PIC group	1339:1366	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	7	8	theme	butyrate-producing	1292:1309	arg1	bacteria					1311:1318	butyrate-producing bacteria	1292:1318	butyrate-producing bacteria	1292:1318	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	6	9	dep	Akkermansiaceae	1087:1101	arg1	intestinalis					1121:1132	intestinalis	1121:1132	intestinalis	1121:1132	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	2	10	theme	piceatannol	344:354	arg1	effect					334:339	the effect	330:339	the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice	330:451	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	7	11	theme	effect	1279:1284	arg1	size					1286:1289	linear discriminant analysis effect size	1250:1289	linear discriminant analysis effect size	1250:1289	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	7	12	theme	linear	1250:1255	arg1	analysis					1270:1277	linear discriminant analysis	1250:1277	linear discriminant analysis effect size	1250:1289	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	9	13	from	ingredient	1606:1615	arg1	development					1624:1634	the development	1620:1634	the development of functional food for colitis prevention	1620:1676	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	9	14	theme	functional	1639:1648	arg1	food					1650:1653	functional food	1639:1653	functional food for colitis prevention	1639:1676	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	5	15	theme	apoptosis-associated	881:900	arg1	expression					910:919	cell apoptosis-associated protein expression	876:919	cell apoptosis-associated protein expression	876:919	The results may be attributed to the effect of PIC on reducing cell apoptosis-associated protein expression, including Bax/Bcl-2 and caspase-3 activation.
36688924	6	16	theme	inhibitory	1149:1158	arg1	effects					1160:1166	inhibitory effects	1149:1166	inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae)	1149:1238	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	0	17	theme	Gut	139:141	arg1	Microbiota					143:152	Gut Microbiota	139:152	Gut Microbiota in Mice	139:160	Piceatannol and 3'-Hydroxypterostilbene Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
36688924	9	18	theme	promising	1596:1604	arg1	ingredient					1606:1615	a promising ingredient	1594:1615	a promising ingredient in the development of functional food for colitis prevention	1594:1676	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	9	18	theme	promising	1596:1604	arg1	it					1588:1589	it	1588:1589	it	1588:1589	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	4	19	theme	tight	796:800	arg1	junctions					802:810	tight junctions	796:810	tight junctions	796:810	However, PIC was comparably effective in modulating tight junctions.
36688924	1	20	theme	significant	203:213	arg1	concern					222:228	a significant health concern	201:228	a significant health concern	201:228	Inflammatory bowel disease has become a significant health concern across the globe, causing frequent and long-term harm to the digestive system.
36688924	7	21	theme	discriminant	1257:1268	arg1	analysis					1270:1277	linear discriminant analysis	1250:1277	linear discriminant analysis effect size	1250:1289	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	9	22	theme	food	1650:1653	arg1	development					1624:1634	the development	1620:1634	the development of functional food for colitis prevention	1620:1676	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	1	23	theme	health	215:220	arg1	concern					222:228	a significant health concern	201:228	a significant health concern	201:228	Inflammatory bowel disease has become a significant health concern across the globe, causing frequent and long-term harm to the digestive system.
36688924	7	24	theme	analysis	1270:1277	arg1	size					1286:1289	linear discriminant analysis effect size	1250:1289	linear discriminant analysis effect size	1250:1289	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	3	25	theme	TNF-α/NF-κB/MLC	676:690	arg1	pathway					692:698	the TNF-α/NF-κB/MLC pathway	672:698	the TNF-α/NF-κB/MLC pathway	672:698	The findings showed that both PIC and HPSB attenuated inflammation by inhibiting the TNF-α/NF-κB/MLC pathway and reducing NLRP3 inflammasome activation.
36688924	5	26	theme	protein	902:908	arg1	expression					910:919	cell apoptosis-associated protein expression	876:919	cell apoptosis-associated protein expression	876:919	The results may be attributed to the effect of PIC on reducing cell apoptosis-associated protein expression, including Bax/Bcl-2 and caspase-3 activation.
36688924	8	27	theme	microbiota	1476:1485	arg1	composition					1487:1497	microbiota composition	1476:1497	microbiota composition	1476:1497	Overall, the results demonstrated that PIC repressed inflammation, inhibited cell apoptosis, and regulated microbiota composition.
36688924	9	28	theme	gut	1551:1553	arg1	integrity					1563:1571	gut barrier integrity	1551:1571	gut barrier integrity	1551:1571	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	2	29	theme	-induced	428:435	arg1	colitis					437:443	dextran sulfate sodium (DSS)-induced colitis	400:443	dextran sulfate sodium (DSS)-induced colitis in mice	400:451	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	9	30	theme	colitis	1659:1665	arg1	prevention					1667:1676	colitis prevention	1659:1676	colitis prevention	1659:1676	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	6	31	theme	bacterial	1179:1187	arg1	Acholeplasmataceae					1220:1237	Acholeplasmataceae	1220:1237	Acholeplasmataceae	1220:1237	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	31	theme	bacterial	1179:1187	arg1	Spiroplasmataceae					1198:1214	Spiroplasmataceae	1198:1214	Spiroplasmataceae	1198:1214	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	31	theme	bacterial	1179:1187	arg1	species					1189:1195	several bacterial species	1171:1195	several bacterial species (Spiroplasmataceae and Acholeplasmataceae)	1171:1238	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	0	32	theme	Bowel	63:67	arg1	Disease					69:75	Inflammatory Bowel Disease	50:75	Inflammatory Bowel Disease	50:75	Piceatannol and 3'-Hydroxypterostilbene Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
36688924	2	33	theme	dysfunction	544:554	arg1	amelioration					516:527	the amelioration	512:527	the amelioration of gut barrier dysfunction	512:554	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	9	34	theme	barrier	1555:1561	arg1	integrity					1563:1571	gut barrier integrity	1551:1571	gut barrier integrity	1551:1571	Consequently, PIC is more effective in maintaining gut barrier integrity than HPSB, and it is a promising ingredient in the development of functional food for colitis prevention.
36688924	5	35	theme	PIC	860:862	arg1	effect					850:855	the effect	846:855	the effect of PIC on reducing cell apoptosis-associated protein expression	846:919	The results may be attributed to the effect of PIC on reducing cell apoptosis-associated protein expression, including Bax/Bcl-2 and caspase-3 activation.
36688924	0	36	theme	Inflammatory	50:61	arg1	Disease					69:75	Inflammatory Bowel Disease	50:75	Inflammatory Bowel Disease	50:75	Piceatannol and 3'-Hydroxypterostilbene Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
36688924	2	37	theme	barrier	536:542	arg1	dysfunction					544:554	gut barrier dysfunction	532:554	gut barrier dysfunction	532:554	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	6	38	theme	microbiota	981:990	arg1	analysis					992:999	microbiota analysis	981:999	microbiota analysis	981:999	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	2	39	theme	colitis	582:588	arg1	severity					570:577	the severity	566:577	the severity of colitis	566:588	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	3	40	theme	NLRP3	713:717	arg1	activation					732:741	NLRP3 inflammasome activation	713:741	NLRP3 inflammasome activation	713:741	The findings showed that both PIC and HPSB attenuated inflammation by inhibiting the TNF-α/NF-κB/MLC pathway and reducing NLRP3 inflammasome activation.
36688924	0	41	from	Microbiota	143:152	arg1	Mice					157:160	Mice	157:160	Mice	157:160	Piceatannol and 3'-Hydroxypterostilbene Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
36688924	5	42	theme	caspase-3	946:954	arg1	activation					956:965	Bax/Bcl-2 and caspase-3 activation	932:965	activation	956:965	The results may be attributed to the effect of PIC on reducing cell apoptosis-associated protein expression, including Bax/Bcl-2 and caspase-3 activation.
36688924	7	43	theme	PIC	1358:1360	arg1	group					1362:1366	the PIC group	1354:1366	the PIC group	1354:1366	Based on linear discriminant analysis effect size, butyrate-producing bacteria were identified as a biomarker in the PIC group.
36688924	8	44	theme	cell	1446:1449	arg1	apoptosis					1451:1459	cell apoptosis	1446:1459	cell apoptosis	1446:1459	Overall, the results demonstrated that PIC repressed inflammation, inhibited cell apoptosis, and regulated microbiota composition.
36688924	6	45	dep	species	1189:1195	arg1	Acholeplasmataceae					1220:1237	Acholeplasmataceae	1220:1237	Acholeplasmataceae	1220:1237	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	45	dep	species	1189:1195	arg1	Spiroplasmataceae					1198:1214	Spiroplasmataceae	1198:1214	Spiroplasmataceae	1198:1214	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	45	dep	species	1189:1195	arg1	species					1189:1195	several bacterial species	1171:1195	several bacterial species (Spiroplasmataceae and Acholeplasmataceae)	1171:1238	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	1	46	theme	Inflammatory	163:174	arg1	disease					182:188	Inflammatory bowel disease	163:188	Inflammatory bowel disease	163:188	Inflammatory bowel disease has become a significant health concern across the globe, causing frequent and long-term harm to the digestive system.
36688924	1	47	theme	digestive	291:299	arg1	system					301:306	the digestive system	287:306	the digestive system	287:306	Inflammatory bowel disease has become a significant health concern across the globe, causing frequent and long-term harm to the digestive system.
36688924	1	48	theme	frequent	256:263	arg1	harm					279:282	frequent and long-term harm	256:282	frequent and long-term harm	256:282	Inflammatory bowel disease has become a significant health concern across the globe, causing frequent and long-term harm to the digestive system.
36688924	0	49	theme	Intestinal	92:101	arg1	Integrity					114:122	Intestinal Epithelial Integrity	92:122	Intestinal Epithelial Integrity	92:122	Piceatannol and 3'-Hydroxypterostilbene Alleviate Inflammatory Bowel Disease by Maintaining Intestinal Epithelial Integrity and Regulating Gut Microbiota in Mice.
36688924	6	50	from	effects	1160:1166	arg1	Acholeplasmataceae					1220:1237	Acholeplasmataceae	1220:1237	Acholeplasmataceae	1220:1237	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	50	from	effects	1160:1166	arg1	Spiroplasmataceae					1198:1214	Spiroplasmataceae	1198:1214	Spiroplasmataceae	1198:1214	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	50	from	effects	1160:1166	arg1	species					1189:1195	several bacterial species	1171:1195	several bacterial species (Spiroplasmataceae and Acholeplasmataceae)	1171:1238	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	1	51	theme	bowel	176:180	arg1	disease					182:188	Inflammatory bowel disease	163:188	Inflammatory bowel disease	163:188	Inflammatory bowel disease has become a significant health concern across the globe, causing frequent and long-term harm to the digestive system.
36688924	5	52	theme	cell	876:879	arg1	expression					910:919	cell apoptosis-associated protein expression	876:919	cell apoptosis-associated protein expression	876:919	The results may be attributed to the effect of PIC on reducing cell apoptosis-associated protein expression, including Bax/Bcl-2 and caspase-3 activation.
36688924	2	53	from	colitis	437:443	arg1	mice					448:451	mice	448:451	mice	448:451	This study evaluated the effect of piceatannol (PIC) and 3'-hydroxypterostilbene (HPSB) on dextran sulfate sodium (DSS)-induced colitis in mice and investigated whether their effects are exerted through the amelioration of gut barrier dysfunction to reduce the severity of colitis.
36688924	6	54	theme	probiotic	1058:1066	arg1	Lactobacillus					1107:1119	Lactobacillus	1107:1119	Lactobacillus	1107:1119	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	54	theme	probiotic	1058:1066	arg1	species					1068:1074	representative probiotic species	1043:1074	representative probiotic species	1043:1074	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	54	theme	probiotic	1058:1066	arg1	Akkermansiaceae					1087:1101	Akkermansiaceae	1087:1101	Akkermansiaceae	1087:1101	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	55	theme	several	1171:1177	arg1	Acholeplasmataceae					1220:1237	Acholeplasmataceae	1220:1237	Acholeplasmataceae	1220:1237	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	55	theme	several	1171:1177	arg1	Spiroplasmataceae					1198:1214	Spiroplasmataceae	1198:1214	Spiroplasmataceae	1198:1214	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	6	55	theme	several	1171:1177	arg1	species					1189:1195	several bacterial species	1171:1195	several bacterial species (Spiroplasmataceae and Acholeplasmataceae)	1171:1238	Furthermore, microbiota analysis revealed that both PIC and HPSB increased representative probiotic species, including Akkermansiaceae and Lactobacillus intestinalis, and exhibited inhibitory effects on several bacterial species (Spiroplasmataceae and Acholeplasmataceae).
36688924	1	56	theme	long-term	269:277	arg1	harm					279:282	frequent and long-term harm	256:282	frequent and long-term harm	256:282	Inflammatory bowel disease has become a significant health concern across the globe, causing frequent and long-term harm to the digestive system.
35725877	4	0	theme	old	836:838	arg1	weight					850:855	32 weeks old mean body weight	827:855	32 weeks old mean body weight	827:855	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	3	1	theme	expressed	663:671	arg1	DEGs					680:683	DEGs	680:683	DEGs	680:683	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	3	1	theme	expressed	663:671	arg1	genes					673:677	differentially expressed genes	648:677	differentially expressed genes (DEGs)	648:684	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	8	2	theme	HSM	1936:1938	arg1	group					1940:1944	HSM group	1936:1944	HSM group	1936:1944	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	5	3	theme	HS	1321:1322	arg1	group					1324:1328	HS group	1321:1328	HS group (P < 0.05)	1321:1339	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	5	3	theme	HS	1321:1322	arg1	P < 0.05					1331:1338	P < 0.05	1331:1338	P < 0.05	1331:1338	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	9	4	from	COX2	2098:2101	arg1	pathway					2120:2126	enriched KEGG pathway	2106:2126	enriched KEGG pathway of NF-kβ pathway	2106:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	4	5	theme	body	845:848	arg1	weight					850:855	32 weeks old mean body weight	827:855	32 weeks old mean body weight	827:855	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	3	6	theme	jejunal	689:695	arg1	mucosa					697:702	jejunal mucosa	689:702	jejunal mucosa of heat-stressed rabbits	689:727	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	6	7	theme	myeloperoxidase	1444:1458	arg1	concentrations					1374:1387	the concentrations	1370:1387	the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase	1370:1458	Furthermore, MOLP decreased the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase compared with HS group (P < 0.05).
35725877	1	8	theme	immune	266:271	arg1	responses					273:281	immune responses	266:281	immune responses in animals	266:292	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	11	9	from	COL3A1	2393:2398	arg1	digestion					2432:2440	protein digestion	2424:2440	protein digestion	2424:2440	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	4	10	theme	White	812:816	arg1	rabbits					818:824	21 male New Zealand White rabbits	792:824	21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g)	792:872	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	8	11	theme	Ruminococcaceae	1788:1802	arg1	group					1812:1816	Ruminococcaceae NK4A214 group	1788:1816	Ruminococcaceae NK4A214 group	1788:1816	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	5	12	theme	jejunal	1294:1300	arg1	mucosa					1302:1307	the jejunal mucosa	1290:1307	the jejunal mucosa	1290:1307	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	1	13	from	responses	273:281	arg1	animals					286:292	animals	286:292	animals	286:292	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	8	14	theme	NK4A136	1851:1857	arg1	group					1859:1863	Lachnospiraceae NK4A136 group	1835:1863	Lachnospiraceae NK4A136 group	1835:1863	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	3	15	theme	rabbits	721:727	arg1	mucosa					697:702	jejunal mucosa	689:702	jejunal mucosa of heat-stressed rabbits	689:727	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	9	16	from	TNFRSF13C	2078:2086	arg1	pathway					2120:2126	enriched KEGG pathway	2106:2126	enriched KEGG pathway of NF-kβ pathway	2106:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	2	17	theme	oleifera	311:318	arg1	MOLP					333:336	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	6	18	theme	pro-inflammatory	1412:1427	arg1	cytokines					1429:1437	pro-inflammatory cytokines	1412:1437	pro-inflammatory cytokines	1412:1437	Furthermore, MOLP decreased the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase compared with HS group (P < 0.05).
35725877	8	19	theme	R-7	1773:1775	arg1	group					1781:1785	R-7 gut group	1773:1785	R-7 gut group	1773:1785	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	2	20	theme	Dietary	295:301	arg1	MOLP					333:336	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	6	21	theme	lipopolysaccharide	1392:1409	arg1	concentrations					1374:1387	the concentrations	1370:1387	the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase	1370:1458	Furthermore, MOLP decreased the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase compared with HS group (P < 0.05).
35725877	10	22	theme	protein	2225:2231	arg1	digestion					2233:2241	protein digestion	2225:2241	protein digestion	2225:2241	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	4	23	theme	MOLP	995:998	arg1	supplementation					1000:1014	MOLP supplementation	995:1014	MOLP supplementation (HSM, 35 °C for 7 h daily) gavage	995:1048	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	3	24	theme	next-generation	742:756	arg1	techniques					769:778	the next-generation sequencing techniques	738:778	the next-generation sequencing techniques	738:778	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	1	25	from	digestive	252:260	arg1	animals					286:292	animals	286:292	animals	286:292	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	3	26	theme	tight	578:582	arg1	barriers					593:600	tight junction barriers	578:600	tight junction barriers	578:600	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	4	27	dep	supplementation	1000:1014	arg1	HSM					1017:1019	HSM	1017:1019	HSM	1017:1019	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	27	dep	supplementation	1000:1014	arg1	gavage					1043:1048	gavage	1043:1048	MOLP supplementation (HSM, 35 °C for 7 h daily) gavage	995:1048	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	27	dep	supplementation	1000:1014	arg1	35 °C					1022:1026	35 °C	1022:1026	35 °C for 7 h daily	1022:1040	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	12	28	theme	jejunal	2531:2537	arg1	permeability					2539:2550	jejunal permeability	2531:2550	jejunal permeability	2531:2550	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	0	29	theme	heat	155:158	arg1	rabbits					169:175	heat stressed rabbits	155:175	heat stressed rabbits	155:175	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	12	30	theme	heat-stressed	2643:2655	arg1	rabbits					2657:2663	heat-stressed rabbits	2643:2663	heat-stressed rabbits	2643:2663	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	7	31	theme	microbiota	1506:1515	arg1	analysis					1517:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	8	32	from	levels	1926:1931	arg1	group					1940:1944	HSM group	1936:1944	HSM group	1936:1944	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	4	33	theme	200 mg/kg	1053:1061	arg1	weight					1068:1073	200 mg/kg body weight	1053:1073	200 mg/kg body weight per day for 4 weeks	1053:1093	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	5	34	theme	mRNA	1154:1157	arg1	expression					1159:1168	mRNA expression	1154:1168	mRNA expression of tight junction proteins	1154:1195	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	9	35	theme	enriched	2106:2113	arg1	pathway					2120:2126	enriched KEGG pathway	2106:2126	enriched KEGG pathway of NF-kβ pathway	2106:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	3	36	theme	intestinal	603:612	arg1	microbiota					614:623	intestinal microbiota	603:623	intestinal microbiota (jejunal digesta)	603:641	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	3	36	theme	intestinal	603:612	arg1	digesta					634:640	jejunal digesta	626:640	jejunal digesta	626:640	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	8	37	theme	higher	1689:1694	arg1	abundance					1696:1704	higher abundance	1689:1704	higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group	1689:1968	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	2	38	from	effects	401:407	arg1	terms					412:416	terms	412:416	terms of intestinal function improvement	412:451	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	9	39	theme	NF-kβ	2131:2135	arg1	pathway					2137:2143	NF-kβ pathway	2131:2143	NF-kβ pathway	2131:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	12	40	dep	improve	2523:2529	arg1	modulate					2587:2594	modulate	2587:2594	modulate microbiota composition and mucosal immunity in heat-stressed rabbits	2587:2663	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	8	41	theme	higher	1910:1915	arg1	levels					1926:1931	higher butyrate levels	1910:1931	higher butyrate levels in HSM group	1910:1944	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	8	42	theme	health-associated	1720:1736	arg1	genera					1738:1743	putatively health-associated genera	1709:1743	putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group	1709:1968	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	5	43	theme	transferase	1213:1223	arg1	activity					1225:1232	glutathione transferase activity	1201:1232	glutathione transferase activity	1201:1232	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	0	44	theme	microbiota	108:117	arg1	composition					119:129	microbiota composition	108:129	microbiota composition	108:129	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	11	45	theme	fibrillar	2366:2374	arg1	collagens					2376:2384	fibrillar collagens	2366:2384	fibrillar collagens	2366:2384	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	0	46	theme	Dietary	0:6	arg1	powder					30:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	10	47	theme	genes	2216:2220	arg1	expression					2202:2211	the expression	2198:2211	the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group	2198:2348	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	7	48	theme	relative	1560:1567	arg1	abundance					1569:1577	the relative abundance	1556:1577	the relative abundance of Bacteroidetes	1556:1594	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	7	48	theme	relative	1560:1567	arg1	higher					1600:1605	higher	1600:1605	higher	1600:1605	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	5	49	theme	junction	1179:1186	arg1	proteins					1188:1195	tight junction proteins	1173:1195	tight junction proteins	1173:1195	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	0	50	theme	oleifera	16:23	arg1	powder					30:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	4	51	from	weight	1068:1073	arg1	control					922:928	control	922:928	control (CON, 25 °C)	922:941	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	51	from	weight	1068:1073	arg1	HS					987:988	HS	987:988	HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks	987:1093	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	51	from	weight	1068:1073	arg1	total					783:787	A total	781:787	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g)	781:872	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	51	from	weight	1068:1073	arg1	stress					949:954	heat stress	944:954	heat stress (HS, 35 °C for 7 h daily)	944:980	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	10	52	theme	HSM	2340:2342	arg1	group					2344:2348	HSM group	2340:2348	HSM group	2340:2348	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	12	53	from	composition	2607:2617	arg1	rabbits					2657:2663	heat-stressed rabbits	2643:2663	heat-stressed rabbits	2643:2663	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	2	54	theme	function	432:439	arg1	improvement					441:451	intestinal function improvement	421:451	intestinal function improvement	421:451	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	9	55	theme	DEGs	1987:1990	arg1	analysis					1975:1982	The analysis	1971:1982	The analysis of DEGs	1971:1990	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	4	56	theme	New	800:802	arg1	rabbits					818:824	21 male New Zealand White rabbits	792:824	21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g)	792:872	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	0	57	theme	jejunal	46:52	arg1	permeability					54:65	jejunal permeability	46:65	jejunal permeability	46:65	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	9	58	from	LBP	2089:2091	arg1	pathway					2120:2126	enriched KEGG pathway	2106:2126	enriched KEGG pathway of NF-kβ pathway	2106:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	4	59	with	control	922:928	arg1	supplementation					1000:1014	MOLP supplementation	995:1014	MOLP supplementation (HSM, 35 °C for 7 h daily) gavage	995:1048	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	60	with	HS	987:988	arg1	supplementation					1000:1014	MOLP supplementation	995:1014	MOLP supplementation (HSM, 35 °C for 7 h daily) gavage	995:1048	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	0	61	from	immunity	143:150	arg1	rabbits					169:175	heat stressed rabbits	155:175	heat stressed rabbits	155:175	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	12	62	from	immunity	2631:2638	arg1	rabbits					2657:2663	heat-stressed rabbits	2643:2663	heat-stressed rabbits	2643:2663	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	10	63	from	digestion	2233:2241	arg1	expression					2202:2211	the expression	2198:2211	the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group	2198:2348	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	8	64	theme	gut	1777:1779	arg1	group					1781:1785	R-7 gut group	1773:1785	R-7 gut group	1773:1785	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	11	65	from	COL12A1	2413:2419	arg1	digestion					2432:2440	protein digestion	2424:2440	protein digestion	2424:2440	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	4	66	theme	32 weeks	827:834	arg1	weight					850:855	32 weeks old mean body weight	827:855	32 weeks old mean body weight	827:855	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	10	67	from	absorption	2247:2256	arg1	expression					2202:2211	the expression	2198:2211	the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group	2198:2348	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	2	68	theme	leaf	320:323	arg1	MOLP					333:336	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	4	69	theme	mean	840:843	arg1	weight					850:855	32 weeks old mean body weight	827:855	32 weeks old mean body weight	827:855	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	70	with	stress	949:954	arg1	supplementation					1000:1014	MOLP supplementation	995:1014	MOLP supplementation (HSM, 35 °C for 7 h daily) gavage	995:1048	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	8	71	dep	Christensenellaceae	1753:1771	arg1	unclassified					1886:1897	unclassified	1886:1897	unclassified along with higher butyrate levels in HSM group as compared to HS group	1886:1968	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	9	72	theme	genes	2063:2067	arg1	downregulation					2045:2058	downregulation	2045:2058	downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway	2045:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	8	73	theme	NK4A214	1804:1810	arg1	group					1812:1816	Ruminococcaceae NK4A214 group	1788:1816	Ruminococcaceae NK4A214 group	1788:1816	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	3	74	theme	current	506:512	arg1	study					514:518	the current study	502:518	the current study	502:518	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	1	75	theme	Heat	178:181	arg1	HS					191:192	HS	191:192	HS	191:192	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	1	75	theme	Heat	178:181	arg1	stress					183:188	Heat stress	178:188	Heat stress (HS)	178:193	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	10	76	dep	digestion	2233:2241	arg1	pathway					2258:2264	pathway	2258:2264	pathway	2258:2264	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	4	77	theme	Zealand	804:810	arg1	rabbits					818:824	21 male New Zealand White rabbits	792:824	21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g)	792:872	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	78	dep	stress	949:954	arg1	35 °C					961:965	35 °C	961:965	35 °C for 7 h daily	961:979	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	78	dep	stress	949:954	arg1	HS					957:958	HS	957:958	HS	957:958	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	3	79	theme	HS	547:548	arg1	impact					537:542	the impact	533:542	the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs)	533:684	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	6	80	theme	cytokines	1429:1437	arg1	concentrations					1374:1387	the concentrations	1370:1387	the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase	1370:1458	Furthermore, MOLP decreased the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase compared with HS group (P < 0.05).
35725877	7	81	theme	phyla	1543:1547	arg1	level					1549:1553	phyla level	1543:1553	phyla level	1543:1553	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	4	82	theme	rabbits	818:824	arg1	stress					949:954	heat stress	944:954	heat stress (HS, 35 °C for 7 h daily)	944:980	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	82	theme	rabbits	818:824	arg1	HS					987:988	HS	987:988	HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks	987:1093	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	82	theme	rabbits	818:824	arg1	total					783:787	A total	781:787	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g)	781:872	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	82	theme	rabbits	818:824	arg1	control					922:928	control	922:928	control (CON, 25 °C)	922:941	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	0	83	theme	mucosal	135:141	arg1	immunity					143:150	mucosal immunity	135:150	mucosal immunity	135:150	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	2	84	theme	Moringa	303:309	arg1	MOLP					333:336	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	12	85	theme	digestive	2556:2564	arg1	function					2566:2573	digestive function	2556:2573	digestive function	2556:2573	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	3	86	theme	heat-stressed	707:719	arg1	rabbits					721:727	heat-stressed rabbits	707:727	heat-stressed rabbits	707:727	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	8	87	theme	Lachnospiraceae	1835:1849	arg1	group					1859:1863	Lachnospiraceae NK4A136 group	1835:1863	Lachnospiraceae NK4A136 group	1835:1863	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	1	88	theme	detrimental	199:209	arg1	effects					211:217	detrimental effects	199:217	detrimental effects	199:217	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	12	89	theme	MOLP	2496:2499	arg1	supplementation					2501:2515	MOLP supplementation	2496:2515	MOLP supplementation	2496:2515	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	3	90	theme	sequencing	758:767	arg1	techniques					769:778	the next-generation sequencing techniques	738:778	the next-generation sequencing techniques	738:778	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	0	91	theme	stressed	160:167	arg1	rabbits					169:175	heat stressed rabbits	155:175	heat stressed rabbits	155:175	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	3	92	theme	MOLP	554:557	arg1	impact					537:542	the impact	533:542	the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs)	533:684	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	7	93	theme	Intestinal	1495:1504	arg1	analysis					1517:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	0	94	from	composition	119:129	arg1	rabbits					169:175	heat stressed rabbits	155:175	heat stressed rabbits	155:175	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	4	95	theme	body	1063:1066	arg1	weight					1068:1073	200 mg/kg body weight	1053:1073	200 mg/kg body weight per day for 4 weeks	1053:1093	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	3	96	from	impact	537:542	arg1	barriers					593:600	tight junction barriers	578:600	tight junction barriers	578:600	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	3	96	from	impact	537:542	arg1	microbiota					614:623	intestinal microbiota	603:623	intestinal microbiota (jejunal digesta)	603:641	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	3	96	from	impact	537:542	arg1	digesta					634:640	jejunal digesta	626:640	jejunal digesta	626:640	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	3	96	from	impact	537:542	arg1	DEGs					680:683	DEGs	680:683	DEGs	680:683	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	3	96	from	impact	537:542	arg1	genes					673:677	differentially expressed genes	648:677	differentially expressed genes (DEGs)	648:684	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	4	97	dep	control	922:928	arg1	25 °C					936:940	25 °C	936:940	25 °C	936:940	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	97	dep	control	922:928	arg1	CON					931:933	CON	931:933	CON	931:933	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	8	98	theme	MOLP	1651:1654	arg1	supplementation					1656:1670	MOLP supplementation	1651:1670	MOLP supplementation	1651:1670	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	7	99	theme	HS	1640:1641	arg1	groups					1643:1648	CON and HS groups	1632:1648	groups	1643:1648	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	12	100	theme	microbiota	2596:2605	arg1	composition					2607:2617	microbiota composition	2596:2617	microbiota composition	2596:2617	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	4	101	theme	3318 ± 171 g	860:871	arg1	weight					850:855	32 weeks old mean body weight	827:855	32 weeks old mean body weight	827:855	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	9	102	theme	KEGG	2115:2118	arg1	pathway					2120:2126	enriched KEGG pathway	2106:2126	enriched KEGG pathway of NF-kβ pathway	2106:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	0	103	theme	digestive	71:79	arg1	function					81:88	digestive function	71:88	digestive function	71:88	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	3	104	theme	junction	584:591	arg1	barriers					593:600	tight junction barriers	578:600	tight junction barriers	578:600	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	6	105	theme	HS	1474:1475	arg1	P < 0.05					1484:1491	P < 0.05	1484:1491	P < 0.05	1484:1491	Furthermore, MOLP decreased the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase compared with HS group (P < 0.05).
35725877	6	105	theme	HS	1474:1475	arg1	group					1477:1481	HS group	1474:1481	HS group (P < 0.05)	1474:1492	Furthermore, MOLP decreased the concentrations of lipopolysaccharide, pro-inflammatory cytokines, and myeloperoxidase compared with HS group (P < 0.05).
35725877	7	106	theme	CON	1632:1634	arg1	groups					1643:1648	CON and HS groups	1632:1648	groups	1643:1648	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	11	107	dep	COL3A1	2393:2398	arg1	i.e.					2387:2390	i.e.	2387:2390	i.e.	2387:2390	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	3	108	dep	HS	547:548	arg1	supplementation					559:573	supplementation	559:573	supplementation	559:573	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	5	109	theme	MOLP	1123:1126	arg1	supplementation					1128:1142	MOLP supplementation	1123:1142	MOLP supplementation	1123:1142	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	9	110	theme	pathway	2137:2143	arg1	pathway					2120:2126	enriched KEGG pathway	2106:2126	enriched KEGG pathway of NF-kβ pathway	2106:2143	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	8	111	theme	butyrate	1917:1924	arg1	levels					1926:1931	higher butyrate levels	1910:1931	higher butyrate levels in HSM group	1910:1944	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	0	112	dep	composition	119:129	arg1	the					104:106	the	104:106	the	104:106	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	7	113	theme	HSM	1610:1612	arg1	group					1614:1618	HSM group	1610:1618	HSM group	1610:1618	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	11	114	theme	collagens	2376:2384	arg1	genes					2357:2361	Three genes	2351:2361	Three genes	2351:2361	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	5	115	theme	glutathione	1201:1211	arg1	activity					1225:1232	glutathione transferase activity	1201:1232	glutathione transferase activity	1201:1232	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	12	116	theme	mucosal	2623:2629	arg1	immunity					2631:2638	mucosal immunity	2623:2638	mucosal immunity	2623:2638	In conclusion, MOLP supplementation could improve jejunal permeability and digestive function, positively modulate microbiota composition and mucosal immunity in heat-stressed rabbits.
35725877	10	117	theme	MOLP	2146:2149	arg1	supplementation					2151:2165	MOLP supplementation	2146:2165	MOLP supplementation	2146:2165	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	0	118	theme	Moringa	8:14	arg1	powder					30:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	8	119	theme	genera	1738:1743	arg1	abundance					1696:1704	higher abundance	1689:1704	higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group	1689:1968	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	7	120	theme	Bacteroidetes	1582:1594	arg1	abundance					1569:1577	the relative abundance	1556:1577	the relative abundance of Bacteroidetes	1556:1594	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	7	120	theme	Bacteroidetes	1582:1594	arg1	higher					1600:1605	higher	1600:1605	higher	1600:1605	Intestinal microbiota analysis revealed that at phyla level, the relative abundance of Bacteroidetes was higher in HSM group compared to CON and HS groups.
35725877	4	121	theme	heat	944:947	arg1	stress					949:954	heat stress	944:954	heat stress (HS, 35 °C for 7 h daily)	944:980	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	4	121	theme	heat	944:947	arg1	total					783:787	A total	781:787	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g)	781:872	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	5	122	theme	tight	1173:1177	arg1	proteins					1188:1195	tight junction proteins	1173:1195	tight junction proteins	1173:1195	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	0	123	theme	leaf	25:28	arg1	powder					30:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder	0:35	Dietary Moringa oleifera leaf powder improves jejunal permeability and digestive function by modulating the microbiota composition and mucosal immunity in heat stressed rabbits.
35725877	3	124	theme	jejunal	626:632	arg1	microbiota					614:623	intestinal microbiota	603:623	intestinal microbiota (jejunal digesta)	603:641	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	3	124	theme	jejunal	626:632	arg1	digesta					634:640	jejunal digesta	626:640	jejunal digesta	626:640	Therefore, the current study investigated the impact of HS and MOLP supplementation on tight junction barriers, intestinal microbiota (jejunal digesta), and differentially expressed genes (DEGs) in jejunal mucosa of heat-stressed rabbits by using the next-generation sequencing techniques.
35725877	5	125	theme	proteins	1188:1195	arg1	activity					1225:1232	glutathione transferase activity	1201:1232	glutathione transferase activity	1201:1232	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	5	125	theme	proteins	1188:1195	arg1	expression					1159:1168	mRNA expression	1154:1168	mRNA expression of tight junction proteins	1154:1195	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35725877	8	126	theme	HS	1961:1962	arg1	group					1964:1968	HS group	1961:1968	HS group	1961:1968	MOLP supplementation also resulted in higher abundance of putatively health-associated genera such as Christensenellaceae R-7 gut group, Ruminococcaceae NK4A214 group, Ruminococcus 2, Lachnospiraceae NK4A136 group, and Lachnospiraceae unclassified along with higher butyrate levels in HSM group as compared to HS group.
35725877	2	127	theme	improvement	441:451	arg1	terms					412:416	terms	412:416	terms of intestinal function improvement	412:451	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	10	128	from	expression	2202:2211	arg1	digestion					2233:2241	protein digestion	2225:2241	protein digestion	2225:2241	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	10	128	from	expression	2202:2211	arg1	group					2344:2348	HSM group	2340:2348	HSM group	2340:2348	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	10	128	from	expression	2202:2211	arg1	absorption					2247:2256	absorption	2247:2256	absorption	2247:2256	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	2	129	theme	HS	389:390	arg1	impact					379:384	the impact	375:384	the impact of HS	375:390	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	2	130	theme	intestinal	421:430	arg1	improvement					441:451	intestinal function improvement	421:451	intestinal function improvement	421:451	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	10	131	from	genes	2216:2220	arg1	digestion					2233:2241	protein digestion	2225:2241	protein digestion	2225:2241	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	10	131	from	genes	2216:2220	arg1	absorption					2247:2256	absorption	2247:2256	absorption	2247:2256	MOLP supplementation also significantly upregulated the expression of genes in protein digestion and absorption pathway, including PRSS2, LOC100349163, CPA1, CPB1, SLC9A3, SLC1A1, and SLC7A9 in HSM group.
35725877	4	132	theme	male	795:798	arg1	rabbits					818:824	21 male New Zealand White rabbits	792:824	21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g)	792:872	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	1	133	contain	has	195:197	arg2	effects					211:217	detrimental effects	199:217	detrimental effects	199:217	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	1	133	contain	has	195:197	arg1	HS					191:192	HS	191:192	HS	191:192	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	1	133	contain	has	195:197	arg1	stress					183:188	Heat stress	178:188	Heat stress (HS)	178:193	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	2	134	theme	powder	325:330	arg1	MOLP					333:336	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP)	295:337	Dietary Moringa oleifera leaf powder (MOLP) has been implicated in ameliorating the impact of HS, but its effects in terms of intestinal function improvement under HS remain poorly characterized.
35725877	1	135	theme	intestinal	222:231	arg1	health					233:238	intestinal health	222:238	intestinal health	222:238	Heat stress (HS) has detrimental effects on intestinal health by altering digestive and immune responses in animals.
35725877	11	136	from	COL5A3	2401:2406	arg1	digestion					2432:2440	protein digestion	2424:2440	protein digestion	2424:2440	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	11	137	theme	HSM	2470:2472	arg1	group					2474:2478	HSM group	2470:2478	HSM group	2470:2478	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	4	138	dep	rabbits	818:824	arg1	weight					850:855	32 weeks old mean body weight	827:855	32 weeks old mean body weight	827:855	A total of 21 male New Zealand White rabbits (32 weeks old mean body weight of 3318 ± 171 g) were divided into three groups (n = 7/group) as control (CON, 25 °C), heat stress (HS, 35 °C for 7 h daily), and HS with MOLP supplementation (HSM, 35 °C for 7 h daily) gavage at 200 mg/kg body weight per day for 4 weeks.
35725877	9	139	theme	inflammatory	2020:2031	arg1	response					2033:2040	inflammatory response	2020:2040	inflammatory response	2020:2040	The analysis of DEGs revealed that MOLP reversed inflammatory response by downregulation of genes, such as TNFRSF13C, LBP, and COX2 in enriched KEGG pathway of NF-kβ pathway.
35725877	11	140	theme	protein	2424:2430	arg1	digestion					2432:2440	protein digestion	2424:2440	protein digestion	2424:2440	Three genes of fibrillar collagens, i.e., COL3A1, COL5A3, and COL12A1 in protein digestion were also down-regulated in HSM group.
35725877	5	141	theme	malonaldehyde	1245:1257	arg1	concentration					1259:1271	the malonaldehyde concentration	1241:1271	the malonaldehyde concentration	1241:1271	The results indicated that MOLP supplementation increased mRNA expression of tight junction proteins and glutathione transferase activity, while the malonaldehyde concentration was decreased in the jejunal mucosa compared to HS group (P < 0.05).
35777933	8	0	theme	coffee	1584:1589	arg1	extracts					1596:1603	coffee leaf extracts	1584:1603	coffee leaf extracts	1584:1603	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	4	1	theme	adapted	744:750	arg1	method					768:773	the adapted drop plate agar method	740:773	the adapted drop plate agar method	740:773	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	7	2	theme	coffee	1479:1484	arg1	by-product					1496:1505	a by-product	1494:1505	a by-product	1494:1505	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	1	3	theme	bioactive	249:257	arg1	compounds					259:267	several bioactive compounds	241:267	several bioactive compounds	241:267	INTRODUCTION Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.
35777933	6	4	theme	bactericidal	1404:1415	arg1	effect					1417:1422	bactericidal effect	1404:1422	bactericidal effect	1404:1422	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	8	5	theme	further	1563:1569	arg1	studies					1571:1577	further studies	1563:1577	further studies with coffee leaf extracts	1563:1603	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	6	6	theme	Gram-negative	1299:1311	arg1	bacteria					1313:1320	Gram-positive and Gram-negative bacteria	1281:1320	Gram-positive and Gram-negative bacteria	1281:1320	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	8	7	with	studies	1571:1577	arg1	extracts					1596:1603	coffee leaf extracts	1584:1603	coffee leaf extracts	1584:1603	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	2	8	theme	antibacterial	352:364	arg1	activity					366:373	the in vitro antibacterial activity	339:373	the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL)	339:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	8	9	with	extract	1664:1670	arg1	activity					1695:1702	the antibacterial activity	1677:1702	the antibacterial activity	1677:1702	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	1	10	theme	compounds	259:267	arg1	sources					230:236	sources	230:236	sources of several bioactive compounds	230:267	INTRODUCTION Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.
35777933	1	10	theme	compounds	259:267	arg1	they					221:224	they	221:224	they	221:224	INTRODUCTION Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.
35777933	5	11	dep	RESULTS	895:901	arg1	analysis					916:923	The chemical analysis	903:923	RESULTS The chemical analysis of EE-CaL	895:933	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	12	theme	EE-CaL	928:933	arg1	analysis					916:923	The chemical analysis	903:923	RESULTS The chemical analysis of EE-CaL	895:933	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	0	13	dep	Coffea	77:82	arg1	L.					92:93	Coffea arabica L.	77:93	Coffea arabica L.	77:93	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	5	14	theme	caffeic	1112:1118	arg1	acid					1083:1086	quinic acid	1076:1086	quinic acid	1076:1086	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	14	theme	caffeic	1112:1118	arg1	acid-O-hexoside					1120:1134	caffeic acid-O-hexoside	1112:1134	caffeic acid-O-hexoside	1112:1134	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	4	15	theme	inhibitory	800:809	arg1	concentration					811:823	the minimum inhibitory concentration	788:823	the minimum inhibitory concentration (MIC)	788:829	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	4	15	theme	inhibitory	800:809	arg1	MIC					826:828	MIC	826:828	MIC	826:828	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	6	16	theme	Gram-positive	1281:1293	arg1	bacteria					1313:1320	Gram-positive and Gram-negative bacteria	1281:1320	Gram-positive and Gram-negative bacteria	1281:1320	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	3	17	theme	quadrupole	577:586	arg1	UPLC-Q-ToF-MS/MS					622:637	UPLC-Q-ToF-MS/MS	622:637	UPLC-Q-ToF-MS/MS	622:637	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	3	17	theme	quadrupole	577:586	arg1	spectrometry					608:619	quadrupole time-of-flight mass spectrometry	577:619	quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS)	577:638	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	8	18	theme	present	1649:1655	arg1	compounds					1639:1647	the compounds	1635:1647	the compounds present in the extract with the antibacterial activity	1635:1702	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	4	19	theme	plate	757:761	arg1	method					768:773	the adapted drop plate agar method	740:773	the adapted drop plate agar method	740:773	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	3	20	theme	chemical	470:477	arg1	characterisation					479:494	The chemical characterisation	466:494	The chemical characterisation of EE-CaL	466:504	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	8	21	from	extract	1664:1670	arg1	present					1649:1655	present	1649:1655	present	1649:1655	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	2	22	dep	OBJECTIVES	270:279	arg1	aimed					292:296	aimed	292:296	aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL)	292:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	0	23	theme	coffee	105:110	arg1	by-product					112:121	A coffee by-product	103:121	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.	0:122	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	5	24	theme	procyanidin	1196:1206	arg1	trimer					1208:1213	procyanidin trimer	1196:1213	procyanidin trimer	1196:1213	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	8	25	attach	present	1649:1655	arg2	compounds					1639:1647	the compounds	1635:1647	the compounds present in the extract with the antibacterial activity	1635:1702	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	8	25	attach	present	1649:1655	arg1	extract					1664:1670	the extract	1660:1670	the extract with the antibacterial activity	1660:1702	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	1	26	dep	INTRODUCTION	124:135	arg1	L.					152:153	L.	152:153	L.	152:153	INTRODUCTION Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.
35777933	8	27	theme	action	1731:1736	arg1	mechanisms					1717:1726	the mechanisms	1713:1726	the mechanisms of action of this extract against bacteria	1713:1769	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	2	28	theme	extract	402:408	arg1	activity					366:373	the in vitro antibacterial activity	339:373	the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL)	339:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	2	28	theme	extract	402:408	arg1	composition					323:333	the chemical composition	310:333	the chemical composition	310:333	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	2	29	theme	ethanol	394:400	arg1	extract					402:408	the lyophilised ethanol extract	378:408	the lyophilised ethanol extract of arabica coffee leaves (EE-CaL)	378:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	0	30	theme	Chemical	0:7	arg1	characterisation					9:24	Chemical characterisation	0:24	Chemical characterisation by UPLC-Q-ToF-MS/MS	0:44	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	3	31	theme	mass	603:606	arg1	UPLC-Q-ToF-MS/MS					622:637	UPLC-Q-ToF-MS/MS	622:637	UPLC-Q-ToF-MS/MS	622:637	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	3	31	theme	mass	603:606	arg1	spectrometry					608:619	quadrupole time-of-flight mass spectrometry	577:619	quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS)	577:638	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	5	32	theme	acid	1083:1086	arg1	catechin					1154:1161	quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin	1076:1161	quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin	1076:1161	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	33	from	presence	948:955	arg1	class					988:992	the alkaloid class	975:992	the alkaloid class	975:992	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	2	34	theme	chemical	314:321	arg1	composition					323:333	the chemical composition	310:333	the chemical composition	310:333	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	7	35	theme	research	1457:1464	arg1	results					1441:1447	The results	1437:1447	The results of this research	1437:1464	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	2	36	theme	lyophilised	382:392	arg1	extract					402:408	the lyophilised ethanol extract	378:408	the lyophilised ethanol extract of arabica coffee leaves (EE-CaL)	378:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	4	37	theme	minimum	839:845	arg1	concentration					860:872	the minimum bactericidal concentration	835:872	the minimum bactericidal concentration (MBC)	835:878	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	4	37	theme	minimum	839:845	arg1	MBC					875:877	MBC	875:877	MBC	875:877	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	6	38	theme	ATCC	1370:1373	arg1	coli					1364:1367	Escherichia coli	1352:1367	Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect)	1352:1423	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	6	38	theme	ATCC	1370:1373	arg1	25922					1375:1379	ATCC 25922	1370:1379	ATCC 25922	1370:1379	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	8	39	theme	leaf	1591:1594	arg1	extracts					1596:1603	coffee leaf extracts	1584:1603	coffee leaf extracts	1584:1603	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	3	40	theme	time-of-flight	588:601	arg1	UPLC-Q-ToF-MS/MS					622:637	UPLC-Q-ToF-MS/MS	622:637	UPLC-Q-ToF-MS/MS	622:637	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	3	40	theme	time-of-flight	588:601	arg1	spectrometry					608:619	quadrupole time-of-flight mass spectrometry	577:619	quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS)	577:638	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	1	41	theme	coffee	197:202	arg1	industry					204:211	the coffee industry	193:211	the coffee industry	193:211	INTRODUCTION Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.
35777933	5	42	theme	alkaloid	979:986	arg1	class					988:992	the alkaloid class	975:992	the alkaloid class	975:992	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	7	43	dep	by-product	1496:1505	arg1	leaves					1486:1491	leaves	1486:1491	leaves	1486:1491	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	2	44	dep	in	343:344	arg1	vitro					346:350	vitro	346:350	vitro	346:350	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	8	45	theme	antibacterial	1681:1693	arg1	activity					1695:1702	the antibacterial activity	1677:1702	the antibacterial activity	1677:1702	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	1	46	theme	industry	204:211	arg1	by-product					179:188	a by-product	177:188	a by-product of the coffee industry	177:211	INTRODUCTION Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.
35777933	0	47	theme	antibacterial	50:62	arg1	potential					64:72	antibacterial potential	50:72	antibacterial potential of Coffea arabica L.	50:93	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	4	48	theme	microdilution	715:727	arg1	method					729:734	the broth microdilution method	705:734	the broth microdilution method	705:734	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	6	49	theme	antibacterial	1230:1242	arg1	potential					1244:1252	the antibacterial potential	1226:1252	the antibacterial potential	1226:1252	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	5	50	theme	5-caffeoylquinic	1089:1104	arg1	acid					1083:1086	quinic acid	1076:1086	quinic acid	1076:1086	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	50	theme	5-caffeoylquinic	1089:1104	arg1	acid					1106:1109	5-caffeoylquinic acid	1089:1109	5-caffeoylquinic acid	1089:1109	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	2	51	dep	coffee	421:426	arg1	leaves					428:433	leaves	428:433	leaves	428:433	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	4	52	theme	broth	709:713	arg1	method					729:734	the broth microdilution method	705:734	the broth microdilution method	705:734	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	2	53	theme	coffee	421:426	arg1	extract					402:408	the lyophilised ethanol extract	378:408	the lyophilised ethanol extract of arabica coffee leaves (EE-CaL)	378:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	5	54	theme	chemical	907:914	arg1	analysis					916:923	The chemical analysis	903:923	RESULTS The chemical analysis of EE-CaL	895:933	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	4	55	theme	drop	752:755	arg1	method					768:773	the adapted drop plate agar method	740:773	the adapted drop plate agar method	740:773	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	4	56	theme	bactericidal	847:858	arg1	concentration					860:872	the minimum bactericidal concentration	835:872	the minimum bactericidal concentration (MBC)	835:878	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	4	56	theme	bactericidal	847:858	arg1	MBC					875:877	MBC	875:877	MBC	875:877	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	2	57	theme	arabica	413:419	arg1	coffee					421:426	arabica coffee leaves	413:433	arabica coffee leaves (EE-CaL)	413:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	2	57	theme	arabica	413:419	arg1	EE-CaL					436:441	EE-CaL	436:441	EE-CaL	436:441	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	7	58	dep	CONCLUSION	1426:1435	arg1	suggest					1466:1472	suggest	1466:1472	suggest that coffee leaves, a by-product, possess compounds with antibacterial properties	1466:1554	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	8	59	theme	extract	1746:1752	arg1	action					1731:1736	action	1731:1736	action of this extract against bacteria	1731:1769	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	5	60	theme	catechin	1169:1176	arg1	monoglucoside					1178:1190	(epi)catechin monoglucoside	1164:1190	(epi)catechin monoglucoside	1164:1190	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	61	theme	phenolic	1049:1056	arg1	catechin					1154:1161	quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin	1076:1161	quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin	1076:1161	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	61	theme	phenolic	1049:1056	arg1	monoglucoside					1178:1190	(epi)catechin monoglucoside	1164:1190	(epi)catechin monoglucoside	1164:1190	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	61	theme	phenolic	1049:1056	arg1	trimer					1208:1213	procyanidin trimer	1196:1213	procyanidin trimer	1196:1213	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	61	theme	phenolic	1049:1056	arg1	compounds					1058:1066	the phenolic compounds	1045:1066	the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer	1045:1213	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	3	62	theme	EE-CaL	499:504	arg1	characterisation					479:494	The chemical characterisation	466:494	The chemical characterisation of EE-CaL	466:504	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	6	63	dep	coli	1364:1367	arg1	MIC = 2500 μg/mL					1383:1398	MIC = 2500 μg/mL	1383:1398	MIC = 2500 μg/mL	1383:1398	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	6	63	dep	coli	1364:1367	arg1	effect					1417:1422	bactericidal effect	1404:1422	bactericidal effect	1404:1422	Regarding the antibacterial potential, EE-CaL was active against Gram-positive and Gram-negative bacteria, being more effective against Escherichia coli (ATCC 25922) (MIC = 2500 μg/mL and bactericidal effect).
35777933	8	64	from	present	1649:1655	arg1	extract					1664:1670	the extract	1660:1670	the extract with the antibacterial activity	1660:1702	Thus, further studies with coffee leaf extracts must be carried out to relate the compounds present in the extract with the antibacterial activity and find the mechanisms of action of this extract against bacteria.
35777933	3	65	dep	MATERIAL	445:452	arg1	characterisation					479:494	The chemical characterisation	466:494	The chemical characterisation of EE-CaL	466:504	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	2	66	theme	in	343:344	arg1	activity					366:373	the in vitro antibacterial activity	339:373	the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL)	339:442	OBJECTIVES This study aimed to evaluate the chemical composition and the in vitro antibacterial activity of the lyophilised ethanol extract of arabica coffee leaves (EE-CaL).
35777933	5	67	from	class	988:992	arg1	compounds					960:968	compounds	960:968	compounds from the alkaloid class	960:992	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	67	from	class	988:992	arg1	presence					948:955	the presence	944:955	the presence of compounds from the alkaloid class	944:992	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	68	theme	epi	1165:1167	arg1	monoglucoside					1178:1190	(epi)catechin monoglucoside	1164:1190	(epi)catechin monoglucoside	1164:1190	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	7	69	theme	antibacterial	1531:1543	arg1	properties					1545:1554	antibacterial properties	1531:1554	antibacterial properties	1531:1554	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	7	70	with	compounds	1516:1524	arg1	properties					1545:1554	antibacterial properties	1531:1554	antibacterial properties	1531:1554	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	5	71	theme	compounds	960:968	arg1	presence					948:955	the presence	944:955	the presence of compounds from the alkaloid class	944:992	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	4	72	theme	minimum	792:798	arg1	concentration					811:823	the minimum inhibitory concentration	788:823	the minimum inhibitory concentration (MIC)	788:829	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	4	72	theme	minimum	792:798	arg1	MIC					826:828	MIC	826:828	MIC	826:828	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	5	73	theme	quinic	1076:1081	arg1	epi					1150:1152	epi	1150:1152	epi	1150:1152	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	73	theme	quinic	1076:1081	arg1	acid					1083:1086	quinic acid	1076:1086	quinic acid	1076:1086	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	73	theme	quinic	1076:1081	arg1	acid					1106:1109	5-caffeoylquinic acid	1089:1109	5-caffeoylquinic acid	1089:1109	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	73	theme	quinic	1076:1081	arg1	acid-O-hexoside					1120:1134	caffeic acid-O-hexoside	1112:1134	caffeic acid-O-hexoside	1112:1134	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	5	73	theme	quinic	1076:1081	arg1	mangiferin					1137:1146	mangiferin	1137:1146	mangiferin	1137:1146	RESULTS The chemical analysis of EE-CaL revealed the presence of compounds from the alkaloid class, such as trigonelline and caffeine, in addition to the phenolic compounds such as quinic acid, 5-caffeoylquinic acid, caffeic acid-O-hexoside, mangiferin, (epi)catechin, (epi)catechin monoglucoside and procyanidin trimer.
35777933	4	74	theme	agar	763:766	arg1	method					768:773	the adapted drop plate agar method	740:773	the adapted drop plate agar method	740:773	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	4	75	theme	in	645:646	arg1	effect					668:673	The in vitro antibacterial effect	641:673	The in vitro antibacterial effect of EE-CaL	641:683	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	4	76	dep	in	645:646	arg1	vitro					648:652	vitro	648:652	vitro	648:652	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	0	77	dep	characterisation	9:24	arg1	leaves					95:100	leaves	95:100	leaves	95:100	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	0	77	dep	characterisation	9:24	arg1	by-product					112:121	A coffee by-product	103:121	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.	0:122	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	4	78	theme	EE-CaL	678:683	arg1	effect					668:673	The in vitro antibacterial effect	641:673	The in vitro antibacterial effect of EE-CaL	641:683	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	3	79	theme	ultra-performance	526:542	arg1	chromatography					551:564	ultra-performance liquid chromatography	526:564	ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS)	526:638	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	7	80	contain	possess	1508:1514	arg1	by-product					1496:1505	a by-product	1494:1505	a by-product	1494:1505	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	7	80	contain	possess	1508:1514	arg2	compounds					1516:1524	compounds	1516:1524	compounds with antibacterial properties	1516:1554	CONCLUSION The results of this research suggest that coffee leaves, a by-product, possess compounds with antibacterial properties.
35777933	0	81	theme	Coffea	77:82	arg1	potential					64:72	antibacterial potential	50:72	antibacterial potential of Coffea arabica L.	50:93	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	0	81	theme	Coffea	77:82	arg1	characterisation					9:24	Chemical characterisation	0:24	Chemical characterisation by UPLC-Q-ToF-MS/MS	0:44	Chemical characterisation by UPLC-Q-ToF-MS/MS and antibacterial potential of Coffea arabica L. leaves: A coffee by-product.
35777933	4	82	theme	antibacterial	654:666	arg1	effect					668:673	The in vitro antibacterial effect	641:673	The in vitro antibacterial effect of EE-CaL	641:683	The in vitro antibacterial effect of EE-CaL was evaluated using the broth microdilution method and the adapted drop plate agar method to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC), respectively.
35777933	3	83	theme	liquid	544:549	arg1	chromatography					551:564	ultra-performance liquid chromatography	526:564	ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS)	526:638	MATERIAL AND METHODS The chemical characterisation of EE-CaL was performed using ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UPLC-Q-ToF-MS/MS).
35777933	1	84	theme	several	241:247	arg1	compounds					259:267	several bioactive compounds	241:267	several bioactive compounds	241:267	INTRODUCTION Coffea arabica L. leaves are considered a by-product of the coffee industry however they are sources of several bioactive compounds.
35994527	7	0	theme	environment	1506:1516	arg1	remodeling					1443:1452	significant EEC mediated remodeling	1418:1452	significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment	1418:1516	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	7	1	theme	mediated	1434:1441	arg1	remodeling					1443:1452	significant EEC mediated remodeling	1418:1452	significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment	1418:1516	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	3	2	theme	specialized	551:561	arg1	membrane					539:546	the endometrial basement membrane	514:546	the endometrial basement membrane	514:546	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	2	theme	specialized	551:561	arg1	layer					563:567	a specialized layer	549:567	a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM	549:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	7	3	theme	gelatin	1491:1497	arg1	environment					1506:1516	the methacrylamide-functionalized gelatin matrix environment	1457:1516	the methacrylamide-functionalized gelatin matrix environment	1457:1516	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	7	4	theme	significant	1418:1428	arg1	remodeling					1443:1452	significant EEC mediated remodeling	1418:1452	significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment	1418:1516	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	6	5	theme	hyaluronic	1277:1286	arg1	acid					1288:1291	hyaluronic acid	1277:1291	hyaluronic acid	1277:1291	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	5	6	theme	tiered	892:897	arg1	approach					899:906	a tiered approach	890:906	a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins	890:1140	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	4	7	theme	microenvironment	701:716	arg1	models					675:680	Bioengineering models	660:680	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane	660:782	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	6	8	theme	increased	1340:1348	arg1	attachment					1354:1363	increased EEC attachment	1340:1363	increased EEC attachment	1340:1363	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	5	9	theme	proteins	1133:1140	arg1	combinations					1087:1098	combinations	1087:1098	combinations of endometrial basement membrane proteins	1087:1140	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	2	10	theme	remodeling	299:308	arg1	processes					310:318	Dynamic remodeling processes	291:318	Dynamic remodeling processes of the endometrial extracellular matrix (ECM)	291:364	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	10	theme	remodeling	299:308	arg1	element					378:384	a major element	370:384	a major element of endometrial homeostasis	370:411	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	6	11	theme	collagen	1264:1271	arg1	V					1273:1273	collagen V	1264:1273	collagen V	1264:1273	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	5	12	theme	gelatin	980:986	arg1	hydrogels					988:996	three-dimensional gelatin hydrogels	962:996	three-dimensional gelatin hydrogels	962:996	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	4	13	theme	basement	766:773	arg1	membrane					775:782	an appropriate endometrial ECM and basement membrane	731:782	membrane	775:782	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	5	14	theme	basement	1115:1122	arg1	proteins					1133:1140	endometrial basement membrane proteins	1103:1140	endometrial basement membrane proteins	1103:1140	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	3	15	theme	endometrial	643:653	arg1	ECM					655:657	the underlying endometrial ECM	628:657	the underlying endometrial ECM	628:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	6	16	theme	tenascin	1240:1247	arg1	C					1249:1249	tenascin C	1240:1249	tenascin C	1240:1249	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	0	17	theme	Cell	68:71	arg1	Attachment					73:82	Epithelial Cell Attachment	57:82	Epithelial Cell Attachment	57:82	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	5	18	theme	high-throughput	930:944	arg1	microarrays					946:956	two-dimensional high-throughput microarrays	914:956	two-dimensional high-throughput microarrays	914:956	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	4	19	theme	appropriate	734:744	arg1	ECM					758:760	an appropriate endometrial ECM and basement membrane	731:782	ECM	758:760	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	0	20	from	Role	0:3	arg1	Expression					103:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression	45:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels	45:133	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	6	21	theme	increased	1366:1374	arg1	intensity					1381:1389	increased CK18 intensity	1366:1389	increased CK18 intensity	1366:1389	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	2	22	theme	endometrial	389:399	arg1	homeostasis					401:411	endometrial homeostasis	389:411	endometrial homeostasis	389:411	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	3	23	theme	critical	462:469	arg1	membrane					539:546	the endometrial basement membrane	514:546	the endometrial basement membrane	514:546	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	23	theme	critical	462:469	arg1	element					471:477	A critical element	460:477	A critical element of this tissue microenvironment	460:509	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	0	24	theme	Extracellular	8:20	arg1	Biomolecules					29:40	Extracellular Matrix Biomolecules	8:40	Extracellular Matrix Biomolecules	8:40	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	5	25	theme	EEC	1020:1022	arg1	attachment					1024:1033	EEC attachment	1020:1033	EEC attachment	1020:1033	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	0	26	theme	Biomolecules	29:40	arg1	Role					0:3	Role	0:3	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.	0:134	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	3	27	theme	tissue	487:492	arg1	microenvironment					494:509	this tissue microenvironment	482:509	this tissue microenvironment	482:509	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	0	28	theme	Endometrial	45:55	arg1	Expression					103:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression	45:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels	45:133	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	7	29	theme	protein	1542:1548	arg1	deposition					1550:1559	nascent protein deposition	1534:1559	nascent protein deposition	1534:1559	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	4	30	theme	epithelial	841:850	arg1	EEC					858:860	endometrial epithelial cell (EEC)	829:861	endometrial epithelial cell (EEC) function	829:870	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	6	31	dep	combinations	1155:1166	arg1	V					1273:1273	collagen V	1264:1273	collagen V	1264:1273	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	6	31	dep	combinations	1155:1166	arg1	C					1192:1192	tenascin C	1183:1192	tenascin C	1183:1192	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	6	31	dep	combinations	1155:1166	arg1	collagen					1312:1319	collagen I	1312:1321	collagen I	1312:1321	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	6	31	dep	combinations	1155:1166	arg1	acid					1233:1236	hyaluronic acid	1222:1236	hyaluronic acid	1222:1236	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	6	31	dep	combinations	1155:1166	arg1	collagen					1169:1176	collagen IV	1169:1179	collagen IV	1169:1179	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	6	31	dep	combinations	1155:1166	arg1	V					1261:1261	collagen V	1252:1261	collagen V	1252:1261	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	6	31	dep	combinations	1155:1166	arg1	collagen					1195:1202	collagen I	1195:1204	collagen I	1195:1204	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	6	31	dep	combinations	1155:1166	arg1	collagen					1294:1301	collagen III	1294:1305	collagen III	1294:1305	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	2	32	theme	extracellular	339:351	arg1	matrix					353:358	the endometrial extracellular matrix	323:358	the endometrial extracellular matrix (ECM)	323:364	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	32	theme	extracellular	339:351	arg1	ECM					361:363	ECM	361:363	ECM	361:363	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	8	33	theme	tissue-engineered	1700:1716	arg1	models					1718:1723	tissue-engineered models	1700:1723	tissue-engineered models of the endometrial microenvironment	1700:1759	Together, we report efforts to tailor the localization of basement membrane-associated proteins and proteoglycans in order to investigate tissue-engineered models of the endometrial microenvironment.
35994527	1	34	theme	menstrual	235:243	arg1	cycle					245:249	the menstrual cycle	231:249	the menstrual cycle	231:249	The endometrium undergoes profound changes in tissue architecture and composition, both during the menstrual cycle as well as in the context of pregnancy.
35994527	1	35	theme	profound	162:169	arg1	changes					171:177	profound changes	162:177	profound changes	162:177	The endometrium undergoes profound changes in tissue architecture and composition, both during the menstrual cycle as well as in the context of pregnancy.
35994527	8	36	theme	membrane-associated	1629:1647	arg1	proteins					1649:1656	basement membrane-associated proteins	1620:1656	basement membrane-associated proteins	1620:1656	Together, we report efforts to tailor the localization of basement membrane-associated proteins and proteoglycans in order to investigate tissue-engineered models of the endometrial microenvironment.
35994527	3	37	theme	endometrial	518:528	arg1	membrane					539:546	the endometrial basement membrane	514:546	the endometrial basement membrane	514:546	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	37	theme	endometrial	518:528	arg1	layer					563:567	a specialized layer	549:567	a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM	549:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	37	theme	endometrial	518:528	arg1	element					471:477	A critical element	460:477	A critical element of this tissue microenvironment	460:509	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	5	38	theme	expression	1061:1070	arg1	patterns					1008:1015	patterns	1008:1015	patterns of EEC attachment and cytokeratin 18 (CK18) expression	1008:1070	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	8	39	theme	endometrial	1732:1742	arg1	microenvironment					1744:1759	the endometrial microenvironment	1728:1759	the endometrial microenvironment	1728:1759	Together, we report efforts to tailor the localization of basement membrane-associated proteins and proteoglycans in order to investigate tissue-engineered models of the endometrial microenvironment.
35994527	7	40	theme	matrix	1499:1504	arg1	environment					1506:1516	the methacrylamide-functionalized gelatin matrix environment	1457:1516	the methacrylamide-functionalized gelatin matrix environment	1457:1516	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	3	41	from	ECM	655:657	arg1	epithelium					612:621	the endometrial epithelium	596:621	the endometrial epithelium from the underlying endometrial ECM	596:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	2	42	theme	Dynamic	291:297	arg1	processes					310:318	Dynamic remodeling processes	291:318	Dynamic remodeling processes of the endometrial extracellular matrix (ECM)	291:364	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	42	theme	Dynamic	291:297	arg1	element					378:384	a major element	370:384	a major element of endometrial homeostasis	370:411	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	7	43	theme	methacrylamide-functionalized	1461:1489	arg1	environment					1506:1516	the methacrylamide-functionalized gelatin matrix environment	1457:1516	the methacrylamide-functionalized gelatin matrix environment	1457:1516	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	6	44	theme	hyaluronic	1222:1231	arg1	acid					1233:1236	hyaluronic acid	1222:1236	hyaluronic acid	1222:1236	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	1	45	theme	tissue	182:187	arg1	architecture					189:200	tissue architecture	182:200	tissue architecture	182:200	The endometrium undergoes profound changes in tissue architecture and composition, both during the menstrual cycle as well as in the context of pregnancy.
35994527	7	46	theme	EEC	1430:1432	arg1	remodeling					1443:1452	significant EEC mediated remodeling	1418:1452	significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment	1418:1516	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	5	47	dep	combinations	1087:1098	arg1	response					1075:1082	response	1075:1082	response	1075:1082	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	3	48	theme	proteins	572:579	arg1	membrane					539:546	the endometrial basement membrane	514:546	the endometrial basement membrane	514:546	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	48	theme	proteins	572:579	arg1	layer					563:567	a specialized layer	549:567	a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM	549:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	4	49	theme	endometrial	689:699	arg1	microenvironment					701:716	the endometrial microenvironment	685:716	the endometrial microenvironment	685:716	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	6	50	theme	EEC	1350:1352	arg1	attachment					1354:1363	increased EEC attachment	1340:1363	increased EEC attachment	1340:1363	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	2	51	theme	matrix	353:358	arg1	processes					310:318	Dynamic remodeling processes	291:318	Dynamic remodeling processes of the endometrial extracellular matrix (ECM)	291:364	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	51	theme	matrix	353:358	arg1	element					378:384	a major element	370:384	a major element of endometrial homeostasis	370:411	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	3	52	theme	endometrial	600:610	arg1	epithelium					612:621	the endometrial epithelium	596:621	the endometrial epithelium from the underlying endometrial ECM	596:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	4	53	theme	Bioengineering	660:673	arg1	models					675:680	Bioengineering models	660:680	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane	660:782	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	5	54	theme	three-dimensional	962:978	arg1	hydrogels					988:996	three-dimensional gelatin hydrogels	962:996	three-dimensional gelatin hydrogels	962:996	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	6	55	theme	collagen	1252:1259	arg1	V					1261:1261	collagen V	1252:1261	collagen V	1252:1261	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	4	56	theme	endometrial	746:756	arg1	ECM					758:760	an appropriate endometrial ECM and basement membrane	731:782	ECM	758:760	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	5	57	theme	endometrial	1103:1113	arg1	proteins					1133:1140	endometrial basement membrane proteins	1103:1140	endometrial basement membrane proteins	1103:1140	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	3	58	theme	underlying	632:641	arg1	ECM					655:657	the underlying endometrial ECM	628:657	the underlying endometrial ECM	628:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	0	59	from	Expression	103:112	arg1	Hydrogels					125:133	Gelatin Hydrogels	117:133	Gelatin Hydrogels	117:133	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	0	60	theme	Attachment	73:82	arg1	Expression					103:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression	45:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels	45:133	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	5	61	theme	membrane	1124:1131	arg1	proteins					1133:1140	endometrial basement membrane proteins	1103:1140	endometrial basement membrane proteins	1103:1140	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	5	62	theme	two-dimensional	914:928	arg1	microarrays					946:956	two-dimensional high-throughput microarrays	914:956	two-dimensional high-throughput microarrays	914:956	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	0	63	theme	Cytokeratin	88:98	arg1	Expression					103:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression	45:112	Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels	45:133	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	6	64	theme	CK18	1376:1379	arg1	intensity					1381:1389	increased CK18 intensity	1366:1389	increased CK18 intensity	1366:1389	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	5	65	theme	attachment	1024:1033	arg1	patterns					1008:1015	patterns	1008:1015	patterns of EEC attachment and cytokeratin 18 (CK18) expression	1008:1070	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	4	66	theme	improved	799:806	arg1	environment					808:818	an improved environment to study endometrial epithelial cell (EEC) function	796:870	an improved environment to study endometrial epithelial cell (EEC) function	796:870	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	0	67	theme	Gelatin	117:123	arg1	Hydrogels					125:133	Gelatin Hydrogels	117:133	Gelatin Hydrogels	117:133	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	5	68	theme	cytokeratin	1039:1049	arg1	expression					1061:1070	cytokeratin 18 (CK18) expression	1039:1070	cytokeratin 18 (CK18) expression	1039:1070	Here, we exploit a tiered approach using two-dimensional high-throughput microarrays and three-dimensional gelatin hydrogels to define patterns of EEC attachment and cytokeratin 18 (CK18) expression in response to combinations of endometrial basement membrane proteins.
35994527	2	69	theme	menstrual	443:451	arg1	cycle					453:457	the menstrual cycle	439:457	the menstrual cycle	439:457	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	70	theme	major	372:376	arg1	processes					310:318	Dynamic remodeling processes	291:318	Dynamic remodeling processes of the endometrial extracellular matrix (ECM)	291:364	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	70	theme	major	372:376	arg1	element					378:384	a major element	370:384	a major element of endometrial homeostasis	370:411	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	70	theme	major	372:376	arg1	changes					424:430	including changes	414:430	including changes across the menstrual cycle	414:457	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	0	71	theme	Matrix	22:27	arg1	Biomolecules					29:40	Extracellular Matrix Biomolecules	8:40	Extracellular Matrix Biomolecules	8:40	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	1	72	theme	pregnancy	280:288	arg1	context					269:275	the context	265:275	the context of pregnancy	265:288	The endometrium undergoes profound changes in tissue architecture and composition, both during the menstrual cycle as well as in the context of pregnancy.
35994527	2	73	theme	including	414:422	arg1	element					378:384	a major element	370:384	a major element of endometrial homeostasis	370:411	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	73	theme	including	414:422	arg1	changes					424:430	including changes	414:430	including changes across the menstrual cycle	414:457	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	4	74	theme	EEC	858:860	arg1	function					863:870	endometrial epithelial cell (EEC) function	829:870	endometrial epithelial cell (EEC) function	829:870	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	2	75	theme	homeostasis	401:411	arg1	processes					310:318	Dynamic remodeling processes	291:318	Dynamic remodeling processes of the endometrial extracellular matrix (ECM)	291:364	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	75	theme	homeostasis	401:411	arg1	element					378:384	a major element	370:384	a major element of endometrial homeostasis	370:411	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	75	theme	homeostasis	401:411	arg1	changes					424:430	including changes	414:430	including changes across the menstrual cycle	414:457	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	0	76	theme	Epithelial	57:66	arg1	Attachment					73:82	Epithelial Cell Attachment	57:82	Epithelial Cell Attachment	57:82	Role of Extracellular Matrix Biomolecules on Endometrial Epithelial Cell Attachment and Cytokeratin 18 Expression on Gelatin Hydrogels.
35994527	8	77	theme	basement	1620:1627	arg1	proteins					1649:1656	basement membrane-associated proteins	1620:1656	basement membrane-associated proteins	1620:1656	Together, we report efforts to tailor the localization of basement membrane-associated proteins and proteoglycans in order to investigate tissue-engineered models of the endometrial microenvironment.
35994527	1	78	dep	architecture	189:200	arg1	both					219:222	both	219:222	both	219:222	The endometrium undergoes profound changes in tissue architecture and composition, both during the menstrual cycle as well as in the context of pregnancy.
35994527	3	79	theme	microenvironment	494:509	arg1	membrane					539:546	the endometrial basement membrane	514:546	the endometrial basement membrane	514:546	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	79	theme	microenvironment	494:509	arg1	element					471:477	A critical element	460:477	A critical element of this tissue microenvironment	460:509	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	7	80	theme	deposition	1550:1559	arg1	analysis					1522:1529	analysis	1522:1529	analysis of nascent protein deposition	1522:1559	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	6	81	theme	tenascin	1183:1190	arg1	C					1192:1192	tenascin C	1183:1192	tenascin C	1183:1192	We identify combinations (collagen IV + tenascin C; collagen I + collagen III; hyaluronic acid + tenascin C; collagen V; collagen V + hyaluronic acid; collagen III; and collagen I) that facilitate increased EEC attachment, increased CK18 intensity, or both.
35994527	8	82	theme	proteins	1649:1656	arg1	localization					1604:1615	the localization	1600:1615	the localization of basement membrane-associated proteins and proteoglycans	1600:1674	Together, we report efforts to tailor the localization of basement membrane-associated proteins and proteoglycans in order to investigate tissue-engineered models of the endometrial microenvironment.
35994527	4	83	theme	endometrial	829:839	arg1	EEC					858:860	endometrial epithelial cell (EEC)	829:861	endometrial epithelial cell (EEC) function	829:870	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	2	84	theme	endometrial	327:337	arg1	matrix					353:358	the endometrial extracellular matrix	323:358	the endometrial extracellular matrix (ECM)	323:364	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	2	84	theme	endometrial	327:337	arg1	ECM					361:363	ECM	361:363	ECM	361:363	Dynamic remodeling processes of the endometrial extracellular matrix (ECM) are a major element of endometrial homeostasis, including changes across the menstrual cycle.
35994527	7	85	theme	nascent	1534:1540	arg1	deposition					1550:1559	nascent protein deposition	1534:1559	nascent protein deposition	1534:1559	We also identify significant EEC mediated remodeling of the methacrylamide-functionalized gelatin matrix environment via analysis of nascent protein deposition.
35994527	8	86	theme	proteoglycans	1662:1674	arg1	localization					1604:1615	the localization	1600:1615	the localization of basement membrane-associated proteins and proteoglycans	1600:1674	Together, we report efforts to tailor the localization of basement membrane-associated proteins and proteoglycans in order to investigate tissue-engineered models of the endometrial microenvironment.
35994527	4	87	theme	cell	852:855	arg1	EEC					858:860	endometrial epithelial cell (EEC)	829:861	endometrial epithelial cell (EEC) function	829:870	Bioengineering models of the endometrial microenvironment that present an appropriate endometrial ECM and basement membrane may provide an improved environment to study endometrial epithelial cell (EEC) function.
35994527	3	88	theme	basement	530:537	arg1	membrane					539:546	the endometrial basement membrane	514:546	the endometrial basement membrane	514:546	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	88	theme	basement	530:537	arg1	layer					563:567	a specialized layer	549:567	a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM	549:657	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	3	88	theme	basement	530:537	arg1	element					471:477	A critical element	460:477	A critical element of this tissue microenvironment	460:509	A critical element of this tissue microenvironment is the endometrial basement membrane, a specialized layer of proteins that separates the endometrial epithelium from the underlying endometrial ECM.
35994527	8	89	theme	microenvironment	1744:1759	arg1	models					1718:1723	tissue-engineered models	1700:1723	tissue-engineered models of the endometrial microenvironment	1700:1759	Together, we report efforts to tailor the localization of basement membrane-associated proteins and proteoglycans in order to investigate tissue-engineered models of the endometrial microenvironment.
37403887	3	0	with	canephora	483:491	arg1	anti-inflammatory					519:535	anti-inflammatory	519:535	anti-inflammatory	519:535	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	3	0	with	canephora	483:491	arg1	antioxidant					506:516	antioxidant	506:516	antioxidant	506:516	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	3	0	with	canephora	483:491	arg1	properties					555:564	regenerative properties	542:564	regenerative properties	542:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	3	1	theme	canephora	483:491	arg1	extract					465:471	a bioactive stem cell extract	443:471	a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties	443:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	7	2	theme	nitric	1092:1097	arg1	NO					1106:1107	NO	1106:1107	NO	1106:1107	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	2	theme	nitric	1092:1097	arg1	oxide					1099:1103	nitric oxide	1092:1103	nitric oxide (NO)	1092:1108	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	2	theme	nitric	1092:1097	arg1	anion					1078:1082	superoxide anion	1067:1082	superoxide anion (O2•-)	1067:1089	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	10	3	theme	High	1422:1425	arg1	content					1436:1442	High phenolic content	1422:1442	High phenolic content	1422:1442	High phenolic content and antioxidant activity were observed in the SCECC.
37403887	6	4	theme	acid	878:881	arg1	methods					795:801	the colorimetric methods	778:801	the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP)	778:937	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	1	5	theme	Plant	163:167	arg1	cultures					174:181	Plant cell cultures	163:181	Plant cell cultures	163:181	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	14	6	from	interest	1869:1876	arg1	cosmetics					1881:1889	cosmetics	1881:1889	cosmetics for preventing skin aging	1881:1915	Hence, it could be of interest in cosmetics for preventing skin aging.
37403887	10	7	theme	phenolic	1427:1434	arg1	content					1436:1442	High phenolic content	1422:1442	High phenolic content	1422:1442	High phenolic content and antioxidant activity were observed in the SCECC.
37403887	0	8	theme	skin	88:91	arg1	properties					106:115	skin regenerative properties	88:115	skin regenerative properties	88:115	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	14	9	theme	skin	1906:1909	arg1	aging					1911:1915	skin aging	1906:1915	skin aging	1906:1915	Hence, it could be of interest in cosmetics for preventing skin aging.
37403887	6	10	theme	2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic	830:876	arg1	ABTS					884:887	ABTS	884:887	ABTS	884:887	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	6	10	theme	2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic	830:876	arg1	acid					878:881	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid	806:881	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS)	806:888	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	13	11	theme	skin	1834:1837	arg1	damage					1839:1844	skin damage	1834:1844	skin damage	1834:1844	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	7	12	theme	B	1211:1211	arg1	production					1053:1062	the production	1049:1062	the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6),	1049:1168	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	12	theme	B	1211:1211	arg1	activity					1178:1185	the activity	1174:1185	the activity of nuclear factor kappa B (NF-κB)	1174:1219	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	5	13	theme	chemical	660:667	arg1	composition					669:679	The chemical composition	656:679	The chemical composition of the extracts	656:695	The chemical composition of the extracts was characterized by mass spectrometry.
37403887	6	14	theme	scavenging	819:828	arg1	ABTS					884:887	ABTS	884:887	ABTS	884:887	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	6	14	theme	scavenging	819:828	arg1	acid					878:881	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid	806:881	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS)	806:888	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	4	15	theme	phenolic	573:580	arg1	Total					567:571	Total phenolic	567:580	Total phenolic	567:580	Total phenolic and flavonoid contents were determined in the SCECC by spectrophotometry.
37403887	7	16	theme	nuclear	1190:1196	arg1	NF-κB					1214:1218	NF-κB	1214:1218	NF-κB	1214:1218	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	16	theme	nuclear	1190:1196	arg1	B					1211:1211	nuclear factor kappa B	1190:1211	nuclear factor kappa B (NF-κB)	1190:1219	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	0	17	from	canephora	36:44	arg1	extract					16:22	Plant stem cell extract	0:22	Plant stem cell extract from Coffea canephora	0:44	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	13	18	theme	natural	1812:1818	arg1	extract					1758:1764	the extract	1754:1764	the extract from C. canephora stem cells	1754:1793	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	13	18	theme	natural	1812:1818	arg1	agent					1820:1824	a natural agent	1810:1824	a natural agent against skin damage	1810:1844	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	3	19	theme	stem	455:458	arg1	extract					465:471	a bioactive stem cell extract	443:471	a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties	443:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	8	20	theme	SCECC	1247:1251	arg1	ability					1236:1242	the ability	1232:1242	the ability of SCECC to stimulate the proliferation and migration of fibroblasts	1232:1311	Moreover, the ability of SCECC to stimulate the proliferation and migration of fibroblasts was assessed.
37403887	1	21	theme	cell	169:172	arg1	cultures					174:181	Plant cell cultures	163:181	Plant cell cultures	163:181	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	0	22	theme	regenerative	93:104	arg1	properties					106:115	skin regenerative properties	88:115	skin regenerative properties	88:115	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	6	23	theme	plasma	925:930	arg1	ability					914:920	the ferric reducing ability	894:920	the ferric reducing ability of plasma (FRAP)	894:937	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	6	23	theme	plasma	925:930	arg1	ABTS					884:887	ABTS	884:887	ABTS	884:887	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	6	23	theme	plasma	925:930	arg1	acid					878:881	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid	806:881	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS)	806:888	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	10	24	theme	antioxidant	1448:1458	arg1	activity					1460:1467	antioxidant activity	1448:1467	antioxidant activity	1448:1467	High phenolic content and antioxidant activity were observed in the SCECC.
37403887	6	25	theme	free	806:809	arg1	scavenging					819:828	free radical scavenging	806:828	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS)	806:888	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	4	26	theme	flavonoid	586:594	arg1	contents					596:603	flavonoid contents	586:603	flavonoid contents	586:603	Total phenolic and flavonoid contents were determined in the SCECC by spectrophotometry.
37403887	11	27	dep	mediators	1595:1603	arg1	IL-6					1626:1629	IL-6	1626:1629	IL-6	1626:1629	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	27	dep	mediators	1595:1603	arg1	NO					1611:1612	NO	1611:1612	NO	1611:1612	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	27	dep	mediators	1595:1603	arg1	O2•-					1605:1608	O2•-	1605:1608	O2•-	1605:1608	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	27	dep	mediators	1595:1603	arg1	mediators					1595:1603	the pro-inflammatory mediators	1574:1603	the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6	1574:1629	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	27	dep	mediators	1595:1603	arg1	TNF-α					1615:1619	TNF-α	1615:1619	TNF-α	1615:1619	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	13	28	theme	C.	1771:1772	arg1	cells					1789:1793	C. canephora stem cells	1771:1793	C. canephora stem cells	1771:1793	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	0	29	theme	stem	6:9	arg1	extract					16:22	Plant stem cell extract	0:22	Plant stem cell extract from Coffea canephora	0:44	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	11	30	theme	dose-dependent	1636:1649	arg1	manner					1651:1656	a dose-dependent manner	1634:1656	a dose-dependent manner	1634:1656	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	7	31	theme	tumor	1111:1115	arg1	factor-alpha					1126:1137	tumor necrosis factor-alpha	1111:1137	tumor necrosis factor-alpha (TNF-α)	1111:1145	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	31	theme	tumor	1111:1115	arg1	anion					1078:1082	superoxide anion	1067:1082	superoxide anion (O2•-)	1067:1089	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	31	theme	tumor	1111:1115	arg1	TNF-α					1140:1144	TNF-α	1140:1144	TNF-α	1140:1144	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	3	32	theme	regenerative	542:553	arg1	properties					555:564	regenerative properties	542:564	regenerative properties	542:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	11	33	theme	pro-inflammatory	1578:1593	arg1	mediators					1595:1603	the pro-inflammatory mediators	1574:1603	the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6	1574:1629	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	33	theme	pro-inflammatory	1578:1593	arg1	NO					1611:1612	NO	1611:1612	NO	1611:1612	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	33	theme	pro-inflammatory	1578:1593	arg1	IL-6					1626:1629	IL-6	1626:1629	IL-6	1626:1629	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	33	theme	pro-inflammatory	1578:1593	arg1	O2•-					1605:1608	O2•-	1605:1608	O2•-	1605:1608	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	33	theme	pro-inflammatory	1578:1593	arg1	TNF-α					1615:1619	TNF-α	1615:1619	TNF-α	1615:1619	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	3	34	theme	unique	404:409	arg1	process					425:431	this unique biotechnology process	399:431	this unique biotechnology process	399:431	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	0	35	theme	Plant	0:4	arg1	extract					16:22	Plant stem cell extract	0:22	Plant stem cell extract from Coffea canephora	0:44	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	7	36	theme	necrosis	1117:1124	arg1	factor-alpha					1126:1137	tumor necrosis factor-alpha	1111:1137	tumor necrosis factor-alpha (TNF-α)	1111:1145	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	36	theme	necrosis	1117:1124	arg1	anion					1078:1082	superoxide anion	1067:1082	superoxide anion (O2•-)	1067:1089	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	36	theme	necrosis	1117:1124	arg1	TNF-α					1140:1144	TNF-α	1140:1144	TNF-α	1140:1144	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	3	37	theme	biotechnology	411:423	arg1	process					425:431	this unique biotechnology process	399:431	this unique biotechnology process	399:431	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	7	38	theme	lipopolysaccharide-stimulated	989:1017	arg1	macrophages					1029:1039	lipopolysaccharide-stimulated RAW 264.7 macrophages	989:1039	lipopolysaccharide-stimulated RAW 264.7 macrophages	989:1039	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	6	39	theme	colorimetric	782:793	arg1	methods					795:801	the colorimetric methods	778:801	the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP)	778:937	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	3	40	theme	bioactive	445:453	arg1	extract					465:471	a bioactive stem cell extract	443:471	a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties	443:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	0	41	theme	cell	11:14	arg1	extract					16:22	Plant stem cell extract	0:22	Plant stem cell extract from Coffea canephora	0:44	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	1	42	theme	last	290:293	arg1	decades					295:301	the last decades	286:301	the last decades	286:301	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	6	43	theme	ability	914:920	arg1	methods					795:801	the colorimetric methods	778:801	the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP)	778:937	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	10	44	located	observed	1474:1481	arg2	activity					1460:1467	antioxidant activity	1448:1467	antioxidant activity	1448:1467	High phenolic content and antioxidant activity were observed in the SCECC.
37403887	10	44	located	observed	1474:1481	arg2	content					1436:1442	High phenolic content	1422:1442	High phenolic content	1422:1442	High phenolic content and antioxidant activity were observed in the SCECC.
37403887	10	44	located	observed	1474:1481	arg1	SCECC					1490:1494	the SCECC	1486:1494	the SCECC	1486:1494	High phenolic content and antioxidant activity were observed in the SCECC.
37403887	7	45	theme	RAW	1019:1021	arg1	macrophages					1029:1039	lipopolysaccharide-stimulated RAW 264.7 macrophages	989:1039	lipopolysaccharide-stimulated RAW 264.7 macrophages	989:1039	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	1	46	theme	promising	197:205	arg1	platform					218:225	a promising production platform	195:225	a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades	195:301	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	6	47	theme	reducing	905:912	arg1	ability					914:920	the ferric reducing ability	894:920	the ferric reducing ability of plasma (FRAP)	894:937	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	12	48	theme	transcription	1695:1707	arg1	factor					1709:1714	the NF-κB transcription factor	1685:1714	the NF-κB transcription factor	1685:1714	Moreover, SCECC inhibited the NF-κB transcription factor.
37403887	1	49	theme	production	207:216	arg1	platform					218:225	a promising production platform	195:225	a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades	195:301	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	3	50	theme	process	425:431	arg1	effectiveness					382:394	the effectiveness	378:394	the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties	378:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	5	51	theme	mass	718:721	arg1	spectrometry					723:734	mass spectrometry	718:734	mass spectrometry	718:734	The chemical composition of the extracts was characterized by mass spectrometry.
37403887	6	52	theme	ferric	898:903	arg1	ability					914:920	the ferric reducing ability	894:920	the ferric reducing ability of plasma (FRAP)	894:937	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	7	53	theme	factor	1198:1203	arg1	NF-κB					1214:1218	NF-κB	1214:1218	NF-κB	1214:1218	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	53	theme	factor	1198:1203	arg1	B					1211:1211	nuclear factor kappa B	1190:1211	nuclear factor kappa B (NF-κB)	1190:1219	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	54	theme	kappa	1205:1209	arg1	NF-κB					1214:1218	NF-κB	1214:1218	NF-κB	1214:1218	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	54	theme	kappa	1205:1209	arg1	B					1211:1211	nuclear factor kappa B	1190:1211	nuclear factor kappa B (NF-κB)	1190:1219	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	0	55	theme	factor-κB	152:160	arg1	suppression					129:139	suppression	129:139	suppression of nuclear factor-κB	129:160	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	13	56	theme	canephora	1774:1782	arg1	cells					1789:1793	C. canephora stem cells	1771:1793	C. canephora stem cells	1771:1793	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	0	57	theme	nuclear	144:150	arg1	factor-κB					152:160	nuclear factor-κB	144:160	nuclear factor-κB	144:160	Plant stem cell extract from Coffea canephora shows antioxidant, anti-inflammatory, and skin regenerative properties mediated by suppression of nuclear factor-κB.
37403887	13	58	used	used	1802:1805	arg2	extract					1758:1764	the extract	1754:1764	the extract from C. canephora stem cells	1754:1793	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	13	58	used	used	1802:1805	arg2	agent					1820:1824	a natural agent	1810:1824	a natural agent against skin damage	1810:1844	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	7	59	theme	superoxide	1067:1076	arg1	factor-alpha					1126:1137	tumor necrosis factor-alpha	1111:1137	tumor necrosis factor-alpha (TNF-α)	1111:1145	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	59	theme	superoxide	1067:1076	arg1	anion					1078:1082	superoxide anion	1067:1082	superoxide anion (O2•-)	1067:1089	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	59	theme	superoxide	1067:1076	arg1	interleukin-6					1148:1160	interleukin-6	1148:1160	interleukin-6 (IL-6)	1148:1167	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	59	theme	superoxide	1067:1076	arg1	oxide					1099:1103	nitric oxide	1092:1103	nitric oxide (NO)	1092:1108	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	59	theme	superoxide	1067:1076	arg1	O2•-					1085:1088	O2•-	1085:1088	O2•-	1085:1088	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	3	60	theme	Coffea	476:481	arg1	SCECC					494:498	SCECC	494:498	SCECC	494:498	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	3	60	theme	Coffea	476:481	arg1	canephora					483:491	Coffea canephora	476:491	Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties	476:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	12	61	theme	NF-κB	1689:1693	arg1	factor					1709:1714	the NF-κB transcription factor	1685:1714	the NF-κB transcription factor	1685:1714	Moreover, SCECC inhibited the NF-κB transcription factor.
37403887	11	62	theme	fibroblasts	1547:1557	arg1	migration					1534:1542	migration	1534:1542	migration	1534:1542	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	11	62	theme	fibroblasts	1547:1557	arg1	proliferation					1516:1528	proliferation	1516:1528	proliferation	1516:1528	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	7	63	theme	anion	1078:1082	arg1	production					1053:1062	the production	1049:1062	the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6),	1049:1168	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	7	63	theme	anion	1078:1082	arg1	activity					1178:1185	the activity	1174:1185	the activity of nuclear factor kappa B (NF-κB)	1174:1219	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	3	64	theme	cell	460:463	arg1	extract					465:471	a bioactive stem cell extract	443:471	a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties	443:564	The study aimed to evaluate the effectiveness of this unique biotechnology process to obtain a bioactive stem cell extract of Coffea canephora (SCECC) with antioxidant, anti-inflammatory, and regenerative properties.
37403887	7	65	theme	anti-inflammatory	944:960	arg1	activity					962:969	The anti-inflammatory activity	940:969	The anti-inflammatory activity	940:969	The anti-inflammatory activity was determined in lipopolysaccharide-stimulated RAW 264.7 macrophages through the production of superoxide anion (O2•-), nitric oxide (NO), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and the activity of nuclear factor kappa B (NF-κB).
37403887	5	66	theme	extracts	688:695	arg1	composition					669:679	The chemical composition	656:679	The chemical composition of the extracts	656:695	The chemical composition of the extracts was characterized by mass spectrometry.
37403887	1	67	dep	biomedical	254:263	arg1	uses					278:281	uses	278:281	uses in the last decades	278:301	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	6	68	theme	radical	811:817	arg1	scavenging					819:828	free radical scavenging	806:828	free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS)	806:888	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	1	69	theme	bioactive	230:238	arg1	compounds					240:248	bioactive compounds	230:248	bioactive compounds	230:248	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	13	70	theme	stem	1784:1787	arg1	cells					1789:1793	C. canephora stem cells	1771:1793	C. canephora stem cells	1771:1793	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	13	71	from	cells	1789:1793	arg1	extract					1758:1764	the extract	1754:1764	the extract from C. canephora stem cells	1754:1793	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	13	71	from	cells	1789:1793	arg1	agent					1820:1824	a natural agent	1810:1824	a natural agent against skin damage	1810:1844	Therefore, we obtained evidence that the extract from C. canephora stem cells can be used as a natural agent against skin damage.
37403887	6	72	theme	Antioxidant	737:747	arg1	activity					749:756	Antioxidant activity	737:756	Antioxidant activity	737:756	Antioxidant activity was evaluated using the colorimetric methods of free radical scavenging 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and the ferric reducing ability of plasma (FRAP).
37403887	8	73	theme	fibroblasts	1301:1311	arg1	migration					1288:1296	migration	1288:1296	migration	1288:1296	Moreover, the ability of SCECC to stimulate the proliferation and migration of fibroblasts was assessed.
37403887	8	73	theme	fibroblasts	1301:1311	arg1	proliferation					1270:1282	proliferation	1270:1282	proliferation	1270:1282	Moreover, the ability of SCECC to stimulate the proliferation and migration of fibroblasts was assessed.
37403887	11	74	dep	proliferation	1516:1528	arg1	the					1512:1514	the	1512:1514	the	1512:1514	SCECC promoted the proliferation and migration of fibroblasts and suppressed the pro-inflammatory mediators O2•-, NO, TNF-α, and IL-6 in a dose-dependent manner.
37403887	1	75	theme	compounds	240:248	arg1	platform					218:225	a promising production platform	195:225	a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades	195:301	Plant cell cultures have become a promising production platform of bioactive compounds for biomedical and cosmetic uses in the last decades.
37403887	9	76	theme	phenolic	1391:1398	arg1	acids					1400:1404	two phenolic acids	1387:1404	two phenolic acids	1387:1404	Five compounds were tentatively identified, two flavonoids, two phenolic acids, and one sugar.
35628422	12	0	theme	lipid	1935:1939	arg1	mediators					1941:1949	lipid mediators	1935:1949	lipid mediators	1935:1949	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	7	1	theme	FLSs	1085:1088	arg1	concentrations					1097:1110	FLSs and HA concentrations	1085:1110	FLSs and HA concentrations in conditioned media	1085:1131	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	1	2	theme	gene	245:248	arg1	conveyors					166:174	conveyors	166:174	conveyors of fatty acids (FAs) and other bioactive lipids	166:222	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	2	theme	gene	245:248	arg1	vesicles					139:146	Extracellular vesicles	125:146	Extracellular vesicles (EVs)	125:152	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	2	theme	gene	245:248	arg1	expression					250:259	gene expression	245:259	gene expression	245:259	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	2	theme	gene	245:248	arg1	behavior					265:272	behavior	265:272	behavior	265:272	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	10	3	from	18:2n-6	1600:1606	arg1	EVs					1611:1613	EVs	1611:1613	EVs	1611:1613	EV counts decreased during cell growth, and 18:2n-6 in EVs correlated with the cell count.
35628422	7	4	theme	hyaluronan	1057:1066	arg1	synthases					1068:1076	hyaluronan synthases 1-3	1057:1080	hyaluronan synthases 1-3	1057:1080	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	0	5	theme	Fatty	0:4	arg1	Fingerprints					11:22	Fatty Acid Fingerprints	0:22	Fatty Acid Fingerprints	0:22	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	1	6	theme	acids	185:189	arg1	expression					250:259	gene expression	245:259	gene expression	245:259	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	6	theme	acids	185:189	arg1	vesicles					139:146	Extracellular vesicles	125:146	Extracellular vesicles (EVs)	125:152	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	6	theme	acids	185:189	arg1	conveyors					166:174	conveyors	166:174	conveyors of fatty acids (FAs) and other bioactive lipids	166:222	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	6	theme	acids	185:189	arg1	behavior					265:272	behavior	265:272	behavior	265:272	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	11	7	theme	20:4n-6	1704:1710	arg1	proportions					1712:1722	increased 20:4n-6 proportions	1694:1722	increased 20:4n-6 proportions	1694:1722	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	11	8	with	FAs	1761:1763	arg1	degree					1776:1781	a low degree	1770:1781	a low degree of unsaturation	1770:1797	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	2	9	theme	lipid	294:298	arg1	composition					300:310	EV lipid composition	291:310	EV lipid composition	291:310	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	8	10	theme	n-6	1237:1239	arg1	PUFAs					1262:1266	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	11	11	theme	unsaturation	1786:1797	arg1	degree					1776:1781	a low degree	1770:1781	a low degree of unsaturation	1770:1797	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	6	12	theme	tracking	899:906	arg1	analysis					908:915	nanoparticle tracking analysis	886:915	nanoparticle tracking analysis	886:915	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	10	13	theme	EV	1556:1557	arg1	counts					1559:1564	EV counts	1556:1564	EV counts	1556:1564	EV counts decreased during cell growth, and 18:2n-6 in EVs correlated with the cell count.
35628422	8	14	theme	FAs	1257:1259	arg1	PUFAs					1262:1266	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	11	15	theme	n-3/n-6	1736:1742	arg1	ratios					1749:1754	reduced n-3/n-6 PUFA ratios	1728:1754	reduced n-3/n-6 PUFA ratios	1728:1754	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	1	16	theme	bioactive	207:215	arg1	lipids					217:222	other bioactive lipids	201:222	other bioactive lipids	201:222	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	8	17	theme	elevated	1195:1202	arg1	proportions					1204:1214	elevated proportions	1195:1214	elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1195:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	18	theme	peroxisome	1404:1413	arg1	-α					1453:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	19	theme	PUFAs	1262:1266	arg1	proportions					1204:1214	elevated proportions	1195:1214	elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1195:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	20	theme	receptor	1438:1445	arg1	-α					1453:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	12	21	from	processes	1969:1977	arg1	joints					1982:1987	joints	1982:1987	joints	1982:1987	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	6	22	theme	sorbent	982:988	arg1	assay					990:994	sandwich-type enzyme-linked sorbent assay	954:994	sandwich-type enzyme-linked sorbent assay	954:994	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	8	23	theme	PPAR	1448:1451	arg1	-α					1453:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	12	24	contain	have	1871:1874	arg2	potential					1880:1888	the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints	1876:1987	the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints	1876:1987	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	12	24	contain	have	1871:1874	arg1	modifications					1857:1869	These FA modifications	1848:1869	These FA modifications	1848:1869	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	5	25	theme	FLSs	825:828	arg1	profiles					813:820	the FA profiles	806:820	the FA profiles of FLSs and EVs during proliferation	806:857	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	6	26	theme	sandwich-type	954:966	arg1	assay					990:994	sandwich-type enzyme-linked sorbent assay	954:994	sandwich-type enzyme-linked sorbent assay	954:994	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	0	27	from	Fingerprints	11:22	arg1	Vesicles					61:68	Extracellular Vesicles	47:68	Extracellular Vesicles	47:68	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	8	28	theme	reductions	1292:1301	arg1	proportions					1204:1214	elevated proportions	1195:1214	elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1195:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	0	29	theme	Proliferating	75:87	arg1	Synoviocytes					111:122	Proliferating Human Fibroblast-like Synoviocytes	75:122	Proliferating Human Fibroblast-like Synoviocytes	75:122	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	5	30	from	changes	739:745	arg1	HA					750:751	HA	750:751	HA	750:751	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	5	30	from	changes	739:745	arg1	secretion					760:768	EV secretion	757:768	EV secretion	757:768	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	5	31	theme	EVs	834:836	arg1	profiles					813:820	the FA profiles	806:820	the FA profiles of FLSs and EVs during proliferation	806:857	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	8	32	theme	n-3/n-6	1306:1312	arg1	ratios					1319:1324	n-3/n-6 PUFA ratios	1306:1324	n-3/n-6 PUFA ratios	1306:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	2	33	dep	fluidity	327:334	arg1	the					323:325	the	323:325	the	323:325	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	11	34	theme	FLS	1660:1662	arg1	proliferation					1664:1676	FLS proliferation	1660:1676	FLS proliferation	1660:1676	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	0	35	theme	Fibroblast-like	95:109	arg1	Synoviocytes					111:122	Proliferating Human Fibroblast-like Synoviocytes	75:122	Proliferating Human Fibroblast-like Synoviocytes	75:122	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	8	36	from	PUFAs	1262:1266	arg1	cells					1285:1289	high-density cells	1272:1289	high-density cells	1272:1289	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	36	from	PUFAs	1262:1266	arg1	ratios					1319:1324	n-3/n-6 PUFA ratios	1306:1324	n-3/n-6 PUFA ratios	1306:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	6	37	theme	laser	927:931	arg1	microscopy					942:951	confocal laser scanning microscopy	918:951	confocal laser scanning microscopy	918:951	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	5	38	theme	study	710:714	arg1	aim					691:693	The aim	687:693	The aim of the present study	687:714	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	8	39	from	reductions	1292:1301	arg1	cells					1285:1289	high-density cells	1272:1289	high-density cells	1272:1289	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	39	from	reductions	1292:1301	arg1	ratios					1319:1324	n-3/n-6 PUFA ratios	1306:1324	n-3/n-6 PUFA ratios	1306:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	4	40	from	inflammation	539:550	arg1	diseases					606:613	joint diseases	600:613	joint diseases	600:613	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	8	41	from	cells	1285:1289	arg1	proportions					1204:1214	elevated proportions	1195:1214	elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1195:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	7	42	from	expression	1043:1052	arg1	concentrations					1097:1110	FLSs and HA concentrations	1085:1110	FLSs and HA concentrations in conditioned media	1085:1131	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	2	43	theme	precursors	414:423	arg1	availability					383:394	the availability	379:394	the availability of lipid mediator precursors	379:423	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	5	44	theme	normal	773:778	arg1	FLSs					780:783	normal FLSs	773:783	normal FLSs	773:783	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	1	45	theme	target	277:282	arg1	cells					284:288	target cells	277:288	target cells	277:288	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	46	theme	Extracellular	125:137	arg1	EVs					149:151	EVs	149:151	EVs	149:151	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	46	theme	Extracellular	125:137	arg1	expression					250:259	gene expression	245:259	gene expression	245:259	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	46	theme	Extracellular	125:137	arg1	vesicles					139:146	Extracellular vesicles	125:146	Extracellular vesicles (EVs)	125:152	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	46	theme	Extracellular	125:137	arg1	conveyors					166:174	conveyors	166:174	conveyors of fatty acids (FAs) and other bioactive lipids	166:222	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	46	theme	Extracellular	125:137	arg1	behavior					265:272	behavior	265:272	behavior	265:272	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	12	47	theme	inflammatory	2090:2101	arg1	diseases					2109:2116	inflammatory joint diseases	2090:2116	inflammatory joint diseases	2090:2116	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	2	48	theme	lipid	399:403	arg1	precursors					414:423	lipid mediator precursors	399:423	lipid mediator precursors	399:423	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	0	49	theme	Hyaluronic	28:37	arg1	Acid					39:42	Hyaluronic Acid	28:42	Hyaluronic Acid	28:42	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	3	50	theme	hyaluronic	489:498	arg1	HA					506:507	HA	506:507	HA	506:507	Fibroblast-like synoviocytes (FLSs) secrete EVs that transport hyaluronic acid (HA).
35628422	3	50	theme	hyaluronic	489:498	arg1	acid					500:503	hyaluronic acid	489:503	hyaluronic acid (HA)	489:508	Fibroblast-like synoviocytes (FLSs) secrete EVs that transport hyaluronic acid (HA).
35628422	7	51	theme	HA	1094:1095	arg1	concentrations					1097:1110	FLSs and HA concentrations	1085:1110	FLSs and HA concentrations in conditioned media	1085:1131	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	8	52	theme	tumor	1379:1383	arg1	factor					1394:1399	tumor necrosis factor α	1379:1401	tumor necrosis factor α	1379:1401	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	1	53	theme	lipids	217:222	arg1	expression					250:259	gene expression	245:259	gene expression	245:259	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	53	theme	lipids	217:222	arg1	vesicles					139:146	Extracellular vesicles	125:146	Extracellular vesicles (EVs)	125:152	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	53	theme	lipids	217:222	arg1	conveyors					166:174	conveyors	166:174	conveyors of fatty acids (FAs) and other bioactive lipids	166:222	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	53	theme	lipids	217:222	arg1	behavior					265:272	behavior	265:272	behavior	265:272	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	9	54	theme	EV	1543:1544	arg1	fraction					1546:1553	the EV fraction	1539:1553	the EV fraction	1539:1553	Compared to the parent FLSs, 16:0, 18:0, and 18:1n-9 were enriched in the EV fraction.
35628422	4	55	theme	potential	649:657	arg1	mediators					659:667	potential mediators	649:667	potential mediators of these effects	649:684	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	4	56	theme	cartilage	575:583	arg1	degradation					585:595	cartilage degradation	575:595	cartilage degradation	575:595	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	8	57	theme	factor	1394:1399	arg1	cluster					1348:1354	cluster	1348:1354	cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1348:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	2	58	theme	membranes	356:364	arg1	fluidity					327:334	fluidity	327:334	fluidity	327:334	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	2	58	theme	membranes	356:364	arg1	stability					340:348	stability	340:348	stability	340:348	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	4	59	from	degradation	585:595	arg1	diseases					606:613	joint diseases	600:613	joint diseases	600:613	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	8	60	from	up-regulation	1331:1343	arg1	cells					1285:1289	high-density cells	1272:1289	high-density cells	1272:1289	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	60	from	up-regulation	1331:1343	arg1	ratios					1319:1324	n-3/n-6 PUFA ratios	1306:1324	n-3/n-6 PUFA ratios	1306:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	61	theme	-α	1453:1454	arg1	cluster					1348:1354	cluster	1348:1354	cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1348:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	12	62	from	biosynthesis	1919:1930	arg1	joints					1982:1987	joints	1982:1987	joints	1982:1987	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	4	63	theme	pannus	553:558	arg1	formation					560:568	pannus formation	553:568	pannus formation	553:568	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	7	64	theme	synthases	1068:1076	arg1	expression					1043:1052	The expression	1039:1052	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media	1039:1131	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	6	65	link	enzyme-linked	968:980	arg1	assay					990:994	sandwich-type enzyme-linked sorbent assay	954:994	sandwich-type enzyme-linked sorbent assay	954:994	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	12	66	from	fluidity	1909:1916	arg1	joints					1982:1987	joints	1982:1987	joints	1982:1987	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	12	67	theme	inflammatory	1956:1967	arg1	processes					1969:1977	inflammatory processes	1956:1977	inflammatory processes in joints	1956:1987	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	1	68	theme	fatty	179:183	arg1	FAs					192:194	FAs	192:194	FAs	192:194	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	68	theme	fatty	179:183	arg1	acids					185:189	fatty acids	179:189	fatty acids (FAs)	179:195	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	12	69	theme	membrane	1900:1907	arg1	fluidity					1909:1916	membrane fluidity	1900:1916	membrane fluidity	1900:1916	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	7	70	dep	synthases	1068:1076	arg1	1-3					1078:1080	1-3	1078:1080	1-3	1078:1080	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	5	71	theme	temporal	730:737	arg1	changes					739:745	temporal changes	730:745	temporal changes in HA and EV secretion	730:768	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	5	72	theme	FA	810:811	arg1	profiles					813:820	the FA profiles	806:820	the FA profiles of FLSs and EVs during proliferation	806:857	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	7	73	from	concentrations	1097:1110	arg1	media					1127:1131	conditioned media	1115:1131	conditioned media	1115:1131	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	6	74	theme	confocal	918:925	arg1	microscopy					942:951	confocal laser scanning microscopy	918:951	confocal laser scanning microscopy	918:951	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	8	75	theme	20:4n-6	1219:1225	arg1	proportions					1204:1214	elevated proportions	1195:1214	elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1195:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	12	76	theme	joint	2103:2107	arg1	diseases					2109:2116	inflammatory joint diseases	2090:2116	inflammatory joint diseases	2090:2116	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	2	77	theme	EV	291:292	arg1	composition					300:310	EV lipid composition	291:310	EV lipid composition	291:310	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	8	78	theme	total	1231:1235	arg1	PUFAs					1262:1266	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	11	79	theme	increased	1694:1702	arg1	proportions					1712:1722	increased 20:4n-6 proportions	1694:1722	increased 20:4n-6 proportions	1694:1722	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	12	80	theme	translational	2029:2041	arg1	studies					2043:2049	translational studies to counteract cartilage degradation in inflammatory joint diseases	2029:2116	translational studies to counteract cartilage degradation in inflammatory joint diseases	2029:2116	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	1	81	theme	other	201:205	arg1	lipids					217:222	other bioactive lipids	201:222	other bioactive lipids	201:222	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	6	82	theme	nanoparticle	886:897	arg1	analysis					908:915	nanoparticle tracking analysis	886:915	nanoparticle tracking analysis	886:915	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	8	83	theme	proliferator-activated	1415:1436	arg1	-α					1453:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	peroxisome proliferator-activated receptor (PPAR)-α	1404:1454	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	84	theme	polyunsaturated	1241:1255	arg1	PUFAs					1262:1266	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	11	85	theme	PUFA	1744:1747	arg1	ratios					1749:1754	reduced n-3/n-6 PUFA ratios	1728:1754	reduced n-3/n-6 PUFA ratios	1728:1754	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	8	86	from	20:4n-6	1219:1225	arg1	cells					1285:1289	high-density cells	1272:1289	high-density cells	1272:1289	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	86	from	20:4n-6	1219:1225	arg1	ratios					1319:1324	n-3/n-6 PUFA ratios	1306:1324	n-3/n-6 PUFA ratios	1306:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	10	87	theme	cell	1635:1638	arg1	count					1640:1644	the cell count	1631:1644	the cell count	1631:1644	EV counts decreased during cell growth, and 18:2n-6 in EVs correlated with the cell count.
35628422	1	88	dep	expression	250:259	arg1	the					241:243	the	241:243	the	241:243	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	4	89	theme	effects	678:684	arg1	mediators					659:667	potential mediators	649:667	potential mediators of these effects	649:684	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	11	90	theme	reduced	1728:1734	arg1	ratios					1749:1754	reduced n-3/n-6 PUFA ratios	1728:1754	reduced n-3/n-6 PUFA ratios	1728:1754	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	8	91	from	proportions	1204:1214	arg1	cells					1285:1289	high-density cells	1272:1289	high-density cells	1272:1289	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	91	from	proportions	1204:1214	arg1	ratios					1319:1324	n-3/n-6 PUFA ratios	1306:1324	n-3/n-6 PUFA ratios	1306:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	0	92	from	Synoviocytes	111:122	arg1	Fingerprints					11:22	Fatty Acid Fingerprints	0:22	Fatty Acid Fingerprints	0:22	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	0	92	from	Synoviocytes	111:122	arg1	Acid					39:42	Hyaluronic Acid	28:42	Hyaluronic Acid	28:42	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	6	93	theme	enzyme-linked	968:980	arg1	assay					990:994	sandwich-type enzyme-linked sorbent assay	954:994	sandwich-type enzyme-linked sorbent assay	954:994	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	8	94	theme	high-density	1272:1283	arg1	cells					1285:1289	high-density cells	1272:1289	high-density cells	1272:1289	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	0	95	theme	Human	89:93	arg1	Synoviocytes					111:122	Proliferating Human Fibroblast-like Synoviocytes	75:122	Proliferating Human Fibroblast-like Synoviocytes	75:122	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	8	96	theme	PPAR-γ	1461:1466	arg1	cluster					1348:1354	cluster	1348:1354	cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1348:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	9	97	theme	parent	1485:1490	arg1	FLSs					1492:1495	the parent FLSs	1481:1495	the parent FLSs	1481:1495	Compared to the parent FLSs, 16:0, 18:0, and 18:1n-9 were enriched in the EV fraction.
35628422	6	98	theme	scanning	933:940	arg1	microscopy					942:951	confocal laser scanning microscopy	918:951	confocal laser scanning microscopy	918:951	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	4	99	from	formation	560:568	arg1	diseases					606:613	joint diseases	600:613	joint diseases	600:613	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	0	100	from	Acid	39:42	arg1	Vesicles					61:68	Extracellular Vesicles	47:68	Extracellular Vesicles	47:68	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	8	101	theme	PUFA	1314:1317	arg1	ratios					1319:1324	n-3/n-6 PUFA ratios	1306:1324	n-3/n-6 PUFA ratios	1306:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	7	102	theme	cell	1150:1153	arg1	proliferation					1155:1167	cell proliferation	1150:1167	cell proliferation	1150:1167	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	5	103	theme	EV	757:758	arg1	secretion					760:768	EV secretion	757:768	EV secretion	757:768	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	6	104	theme	gas	1019:1021	arg1	chromatography					1023:1036	gas chromatography	1019:1036	gas chromatography	1019:1036	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	11	105	theme	low	1772:1774	arg1	degree					1776:1781	a low degree	1770:1781	a low degree of unsaturation	1770:1797	To conclude, FLS proliferation was featured by increased 20:4n-6 proportions and reduced n-3/n-6 PUFA ratios, and FAs with a low degree of unsaturation were selectively transferred from FLSs into EVs.
35628422	3	106	theme	Fibroblast-like	426:440	arg1	synoviocytes					442:453	Fibroblast-like synoviocytes	426:453	Fibroblast-like synoviocytes (FLSs)	426:460	Fibroblast-like synoviocytes (FLSs) secrete EVs that transport hyaluronic acid (HA).
35628422	3	106	theme	Fibroblast-like	426:440	arg1	FLSs					456:459	FLSs	456:459	FLSs	456:459	Fibroblast-like synoviocytes (FLSs) secrete EVs that transport hyaluronic acid (HA).
35628422	7	107	theme	conditioned	1115:1125	arg1	media					1127:1131	conditioned media	1115:1131	conditioned media	1115:1131	The expression of hyaluronan synthases 1-3 in FLSs and HA concentrations in conditioned media decreased during cell proliferation.
35628422	8	108	theme	up-regulation	1331:1343	arg1	proportions					1204:1214	elevated proportions	1195:1214	elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1195:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	2	109	theme	mediator	405:412	arg1	precursors					414:423	lipid mediator precursors	399:423	lipid mediator precursors	399:423	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	1	110	theme	cells	284:288	arg1	conveyors					166:174	conveyors	166:174	conveyors of fatty acids (FAs) and other bioactive lipids	166:222	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	110	theme	cells	284:288	arg1	behavior					265:272	behavior	265:272	behavior	265:272	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	110	theme	cells	284:288	arg1	expression					250:259	gene expression	245:259	gene expression	245:259	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	1	110	theme	cells	284:288	arg1	vesicles					139:146	Extracellular vesicles	125:146	Extracellular vesicles (EVs)	125:152	Extracellular vesicles (EVs) function as conveyors of fatty acids (FAs) and other bioactive lipids and can modulate the gene expression and behavior of target cells.
35628422	6	111	theme	quantitative	997:1008	arg1	PCR					1010:1012	quantitative PCR	997:1012	quantitative PCR	997:1012	The methods used included nanoparticle tracking analysis, confocal laser scanning microscopy, sandwich-type enzyme-linked sorbent assay, quantitative PCR, and gas chromatography.
35628422	12	112	theme	mediators	1941:1949	arg1	biosynthesis					1919:1930	biosynthesis	1919:1930	biosynthesis of lipid mediators	1919:1949	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	12	112	theme	mediators	1941:1949	arg1	fluidity					1909:1916	membrane fluidity	1900:1916	membrane fluidity	1900:1916	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	12	112	theme	mediators	1941:1949	arg1	processes					1969:1977	inflammatory processes	1956:1977	inflammatory processes in joints	1956:1987	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	8	113	theme	cluster	1348:1354	arg1	20:4n-6					1219:1225	20:4n-6	1219:1225	20:4n-6	1219:1225	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	113	theme	cluster	1348:1354	arg1	up-regulation					1331:1343	up-regulation	1331:1343	up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1331:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	113	theme	cluster	1348:1354	arg1	PUFAs					1262:1266	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	total n-6 polyunsaturated FAs (PUFAs)	1231:1267	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	113	theme	cluster	1348:1354	arg1	reductions					1292:1301	reductions	1292:1301	reductions in n-3/n-6 PUFA ratios	1292:1324	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	10	114	theme	cell	1583:1586	arg1	growth					1588:1593	cell growth	1583:1593	cell growth	1583:1593	EV counts decreased during cell growth, and 18:2n-6 in EVs correlated with the cell count.
35628422	0	115	theme	Extracellular	47:59	arg1	Vesicles					61:68	Extracellular Vesicles	47:68	Extracellular Vesicles	47:68	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	4	116	theme	central	523:529	arg1	role					531:534	a central role	521:534	a central role	521:534	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	8	117	theme	differentiation	1359:1373	arg1	cluster					1348:1354	cluster	1348:1354	cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1348:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	8	118	from	ratios	1319:1324	arg1	proportions					1204:1214	elevated proportions	1195:1214	elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ	1195:1466	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	12	119	theme	cartilage	2065:2073	arg1	degradation					2075:2085	cartilage degradation	2065:2085	cartilage degradation in inflammatory joint diseases	2065:2116	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	5	120	theme	present	702:708	arg1	study					710:714	the present study	698:714	the present study	698:714	The aim of the present study was to follow temporal changes in HA and EV secretion by normal FLSs, and to characterize the FA profiles of FLSs and EVs during proliferation.
35628422	2	121	theme	EV	353:354	arg1	membranes					356:364	EV membranes	353:364	EV membranes	353:364	EV lipid composition influences the fluidity and stability of EV membranes and reflects the availability of lipid mediator precursors.
35628422	4	122	theme	joint	600:604	arg1	diseases					606:613	joint diseases	600:613	joint diseases	600:613	FLSs play a central role in inflammation, pannus formation, and cartilage degradation in joint diseases, and EVs have recently emerged as potential mediators of these effects.
35628422	12	123	theme	FA	1854:1855	arg1	modifications					1857:1869	These FA modifications	1848:1869	These FA modifications	1848:1869	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35628422	8	124	theme	necrosis	1385:1392	arg1	factor					1394:1399	tumor necrosis factor α	1379:1401	tumor necrosis factor α	1379:1401	This was associated with elevated proportions of 20:4n-6 and total n-6 polyunsaturated FAs (PUFAs) in high-density cells, reductions in n-3/n-6 PUFA ratios, and up-regulation of cluster of differentiation 44, tumor necrosis factor α, peroxisome proliferator-activated receptor (PPAR)-α, and PPAR-γ.
35628422	0	125	theme	Acid	6:9	arg1	Fingerprints					11:22	Fatty Acid Fingerprints	0:22	Fatty Acid Fingerprints	0:22	Fatty Acid Fingerprints and Hyaluronic Acid in Extracellular Vesicles from Proliferating Human Fibroblast-like Synoviocytes.
35628422	12	126	from	degradation	2075:2085	arg1	diseases					2109:2116	inflammatory joint diseases	2090:2116	inflammatory joint diseases	2090:2116	These FA modifications have the potential to affect membrane fluidity, biosynthesis of lipid mediators, and inflammatory processes in joints, and could eventually provide tools for translational studies to counteract cartilage degradation in inflammatory joint diseases.
35190784	5	0	theme	healthy	757:763	arg1	weight					860:865	normal weight	853:865	normal weight (n = 10)	853:874	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	5	0	theme	healthy	757:763	arg1	obese					835:839	obese	835:839	obese	835:839	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	5	0	theme	healthy	757:763	arg1	dogs					765:768	Twenty clinically healthy dogs	739:768	Twenty clinically healthy dogs	739:768	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	6	1	theme	rRNA	965:968	arg1	region					976:981	the 16S rRNA V3-V4 region	957:981	the 16S rRNA V3-V4 region	957:981	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	9	2	from	differences	1414:1424	arg1	microbiome					1437:1446	the gut microbiome	1429:1446	the gut microbiome	1429:1446	These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
35190784	1	3	theme	multifactorial	136:149	arg1	disorder					163:170	a multifactorial nutritional disorder	134:170	a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries	134:243	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	1	3	theme	multifactorial	136:149	arg1	Obesity					123:129	Obesity	123:129	Obesity	123:129	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	9	4	theme	obese	1474:1478	arg1	dogs					1480:1483	obese dogs	1474:1483	obese dogs	1474:1483	These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
35190784	0	5	theme	microbiome	73:82	arg1	composition					19:29	composition	19:29	composition	19:29	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	0	5	theme	microbiome	73:82	arg1	functions					45:53	predicted functions	35:53	predicted functions	35:53	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	1	6	theme	nutritional	151:161	arg1	disorder					163:170	a multifactorial nutritional disorder	134:170	a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries	134:243	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	1	6	theme	nutritional	151:161	arg1	Obesity					123:129	Obesity	123:129	Obesity	123:129	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	3	7	theme	intestinal	431:440	arg1	microbiome					442:451	The intestinal microbiome	427:451	The intestinal microbiome of obese animals	427:468	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	0	8	from	Differences	0:10	arg1	composition					19:29	composition	19:29	composition	19:29	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	0	8	from	Differences	0:10	arg1	functions					45:53	predicted functions	35:53	predicted functions	35:53	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	3	9	theme	capable	522:528	arg1	members					514:520	certain members	506:520	certain members capable of extracting energy from complex polysaccharides	506:578	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	4	10	theme	dogs	733:736	arg1	microbiome					681:690	the intestinal microbiome	666:690	the intestinal microbiome of Chilean obese and normal weight adult dogs	666:736	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	0	11	theme	obese	87:91	arg1	dogs					117:120	obese and normal weight adult dogs	87:120	obese and normal weight adult dogs	87:120	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	5	12	theme	body	805:808	arg1	BCS					827:829	BCS	827:829	BCS	827:829	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	5	12	theme	body	805:808	arg1	score					820:824	their body condition score	799:824	their body condition score (BCS)	799:830	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	9	13	theme	further	1496:1502	arg1	research					1504:1511	further research	1496:1511	further research to improve animal health by modulating the gut microbiome	1496:1569	These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
35190784	6	14	theme	next-generation	927:941	arg1	sequencing					943:952	next-generation sequencing	927:952	next-generation sequencing of the 16S rRNA V3-V4 region	927:981	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	0	15	theme	weight	104:109	arg1	dogs					117:120	obese and normal weight adult dogs	87:120	obese and normal weight adult dogs	87:120	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	4	16	theme	adult	727:731	arg1	dogs					733:736	Chilean obese and normal weight adult dogs	695:736	Chilean obese and normal weight adult dogs	695:736	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	3	17	theme	certain	506:512	arg1	members					514:520	certain members	506:520	certain members capable of extracting energy from complex polysaccharides	506:578	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	2	18	theme	chronic	363:369	arg1	diseases					417:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	0	19	theme	normal	97:102	arg1	dogs					117:120	obese and normal weight adult dogs	87:120	obese and normal weight adult dogs	87:120	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	7	20	from	anincrease	1143:1152	arg1	Firmicutes					1157:1166	Firmicutes	1157:1166	Firmicutes	1157:1166	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	7	20	from	anincrease	1143:1152	arg1	dogs					1209:1212	obese dogs	1203:1212	obese dogs	1203:1212	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	7	21	theme	phylum	1124:1129	arg1	level					1131:1135	the phylum level	1120:1135	the phylum level	1120:1135	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	3	22	theme	members	514:520	arg1	abundance					493:501	the abundance	489:501	the abundance of certain members capable of extracting energy from complex polysaccharides	489:578	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	9	23	theme	gut	1556:1558	arg1	microbiome					1560:1569	the gut microbiome	1552:1569	the gut microbiome	1552:1569	These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
35190784	2	24	theme	population	290:299	arg1	%					274:274	over 40%	267:274	over 40% of the canine population	267:299	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	2	24	theme	population	290:299	arg1	population					290:299	the canine population	279:299	the canine population	279:299	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	6	25	theme	region	976:981	arg1	analysis					1002:1009	bioinformatics analysis	987:1009	bioinformatics analysis targeting microbiome composition and function	987:1055	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	6	25	theme	region	976:981	arg1	sequencing					943:952	next-generation sequencing	927:952	next-generation sequencing of the 16S rRNA V3-V4 region	927:981	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	1	26	theme	prevalent	179:187	arg1	disorder					163:170	a multifactorial nutritional disorder	134:170	a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries	134:243	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	1	26	theme	prevalent	179:187	arg1	Obesity					123:129	Obesity	123:129	Obesity	123:129	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	2	27	theme	diseases	417:424	arg1	risk					355:358	an increased risk	342:358	an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases	342:424	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	7	28	theme	Significant	1058:1068	arg1	differences					1070:1080	Significant differences	1058:1080	Significant differences	1058:1080	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	2	29	theme	canine	283:288	arg1	population					290:299	the canine population	279:299	the canine population	279:299	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	8	30	theme	related	1328:1334	arg1	pathways					1319:1326	pathways	1319:1326	pathways related to transport, chemotaxis, and flagellar assembly	1319:1383	Microbiome compositions of these animals correlated with their BCS, and obese dogs showed enrichment in pathways related to transport, chemotaxis, and flagellar assembly.
35190784	2	31	theme	increased	345:353	arg1	risk					355:358	an increased risk	342:358	an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases	342:424	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	2	32	theme	cardiovascular	402:415	arg1	diseases					417:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	4	33	theme	weight	720:725	arg1	dogs					733:736	Chilean obese and normal weight adult dogs	695:736	Chilean obese and normal weight adult dogs	695:736	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	0	34	theme	dogs	117:120	arg1	microbiome					73:82	the intestinal microbiome	58:82	the intestinal microbiome of obese and normal weight adult dogs	58:120	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	3	35	from	increases	476:484	arg1	abundance					493:501	the abundance	489:501	the abundance of certain members capable of extracting energy from complex polysaccharides	489:578	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	9	36	theme	gut	1433:1435	arg1	microbiome					1437:1446	the gut microbiome	1429:1446	the gut microbiome	1429:1446	These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
35190784	3	37	theme	complex	556:562	arg1	polysaccharides					564:578	complex polysaccharides	556:578	complex polysaccharides	556:578	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	0	38	theme	adult	111:115	arg1	dogs					117:120	obese and normal weight adult dogs	87:120	obese and normal weight adult dogs	87:120	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	6	39	dep	extracted	885:893	arg1	followed					915:922	followed	915:922	followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function	915:1055	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	4	40	theme	microbiome	681:690	arg1	composition					628:638	composition	628:638	composition	628:638	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	4	40	theme	microbiome	681:690	arg1	function					654:661	predicted function	644:661	predicted function	644:661	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	7	41	located	observed	1087:1094	arg1	level					1131:1135	the phylum level	1120:1135	the phylum level	1120:1135	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	7	41	located	observed	1087:1094	arg2	differences					1070:1080	Significant differences	1058:1080	Significant differences	1058:1080	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	2	42	theme	metabolic	387:395	arg1	diseases					417:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	0	43	theme	predicted	35:43	arg1	functions					45:53	predicted functions	35:53	predicted functions	35:53	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	4	44	dep	composition	628:638	arg1	the					624:626	the	624:626	the	624:626	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	6	45	theme	microbiome	1021:1030	arg1	composition					1032:1042	microbiome composition	1021:1042	microbiome composition	1021:1042	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	6	46	theme	bioinformatics	987:1000	arg1	analysis					1002:1009	bioinformatics analysis	987:1009	bioinformatics analysis targeting microbiome composition and function	987:1055	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	4	47	theme	predicted	644:652	arg1	function					654:661	predicted function	644:661	predicted function	644:661	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	6	48	theme	stool	900:904	arg1	samples					906:912	stool samples	900:912	stool samples	900:912	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	9	49	theme	normal	1456:1461	arg1	weight					1463:1468	normal weight	1456:1468	normal weight	1456:1468	These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
35190784	5	50	theme	condition	810:818	arg1	BCS					827:829	BCS	827:829	BCS	827:829	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	5	50	theme	condition	810:818	arg1	score					820:824	their body condition score	799:824	their body condition score (BCS)	799:830	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	2	51	theme	osteoarticular	371:384	arg1	diseases					417:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	chronic osteoarticular, metabolic, and cardiovascular diseases	363:424	It is estimated that over 40% of the canine population suffers from obesity, which manifests in an increased risk of chronic osteoarticular, metabolic, and cardiovascular diseases.
35190784	7	52	from	decrease	1174:1181	arg1	Firmicutes					1157:1166	Firmicutes	1157:1166	Firmicutes	1157:1166	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	7	52	from	decrease	1174:1181	arg1	dogs					1209:1212	obese dogs	1203:1212	obese dogs	1203:1212	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	8	53	theme	Microbiome	1215:1224	arg1	compositions					1226:1237	Microbiome compositions	1215:1237	Microbiome compositions of these animals	1215:1254	Microbiome compositions of these animals correlated with their BCS, and obese dogs showed enrichment in pathways related to transport, chemotaxis, and flagellar assembly.
35190784	4	54	theme	obese	703:707	arg1	dogs					733:736	Chilean obese and normal weight adult dogs	695:736	Chilean obese and normal weight adult dogs	695:736	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	8	55	theme	flagellar	1366:1374	arg1	assembly					1376:1383	flagellar assembly	1366:1383	flagellar assembly	1366:1383	Microbiome compositions of these animals correlated with their BCS, and obese dogs showed enrichment in pathways related to transport, chemotaxis, and flagellar assembly.
35190784	1	56	from	dogs	192:195	arg1	prevalent					179:187	prevalent	179:187	prevalent	179:187	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	4	57	theme	Chilean	695:701	arg1	dogs					733:736	Chilean obese and normal weight adult dogs	695:736	Chilean obese and normal weight adult dogs	695:736	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	0	58	dep	composition	19:29	arg1	the					15:17	the	15:17	the	15:17	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	6	59	theme	V3-V4	970:974	arg1	region					976:981	the 16S rRNA V3-V4 region	957:981	the 16S rRNA V3-V4 region	957:981	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	3	60	theme	obese	456:460	arg1	animals					462:468	obese animals	456:468	obese animals	456:468	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	1	61	theme	developed	210:218	arg1	countries					235:243	developed and developing countries	210:243	countries	235:243	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	8	62	theme	obese	1287:1291	arg1	dogs					1293:1296	obese dogs	1287:1296	obese dogs	1287:1296	Microbiome compositions of these animals correlated with their BCS, and obese dogs showed enrichment in pathways related to transport, chemotaxis, and flagellar assembly.
35190784	1	63	from	prevalent	179:187	arg1	dogs					192:195	dogs	192:195	dogs	192:195	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	3	64	theme	animals	462:468	arg1	microbiome					442:451	The intestinal microbiome	427:451	The intestinal microbiome of obese animals	427:468	The intestinal microbiome of obese animals shows increases in the abundance of certain members capable of extracting energy from complex polysaccharides.
35190784	4	65	theme	normal	713:718	arg1	dogs					733:736	Chilean obese and normal weight adult dogs	695:736	Chilean obese and normal weight adult dogs	695:736	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	0	66	theme	intestinal	62:71	arg1	microbiome					73:82	the intestinal microbiome	58:82	the intestinal microbiome of obese and normal weight adult dogs	58:120	Differences in the composition and predicted functions of the intestinal microbiome of obese and normal weight adult dogs.
35190784	1	67	located	observed	198:205	arg1	countries					235:243	developed and developing countries	210:243	countries	235:243	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	1	67	located	observed	198:205	arg2	dogs					192:195	dogs	192:195	dogs	192:195	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	8	68	theme	animals	1248:1254	arg1	compositions					1226:1237	Microbiome compositions	1215:1237	Microbiome compositions of these animals	1215:1254	Microbiome compositions of these animals correlated with their BCS, and obese dogs showed enrichment in pathways related to transport, chemotaxis, and flagellar assembly.
35190784	4	69	theme	intestinal	670:679	arg1	microbiome					681:690	the intestinal microbiome	666:690	the intestinal microbiome of Chilean obese and normal weight adult dogs	666:736	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35190784	1	70	theme	developing	224:233	arg1	countries					235:243	developed and developing countries	210:243	countries	235:243	Obesity is a multifactorial nutritional disorder highly prevalent in dogs, observed in developed and developing countries.
35190784	7	71	theme	obese	1203:1207	arg1	dogs					1209:1212	obese dogs	1203:1212	obese dogs	1203:1212	Significant differences were observed between these groups at the phylum level, with anincrease in Firmicutes and a decrease in Bacteroidetes in obese dogs.
35190784	6	72	theme	16S	961:963	arg1	region					976:981	the 16S rRNA V3-V4 region	957:981	the 16S rRNA V3-V4 region	957:981	DNA was extracted from stool samples, followed by next-generation sequencing of the 16S rRNA V3-V4 region and bioinformatics analysis targeting microbiome composition and function.
35190784	9	73	theme	animal	1524:1529	arg1	health					1531:1536	animal health	1524:1536	animal health	1524:1536	These results highlight the differences in the gut microbiome between normal weight and obese dogs and prompt further research to improve animal health by modulating the gut microbiome.
35190784	5	74	theme	normal	853:858	arg1	weight					860:865	normal weight	853:865	normal weight (n = 10)	853:874	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	5	74	theme	normal	853:858	arg1	n = 10					868:873	n = 10	868:873	n = 10	868:873	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	5	74	theme	normal	853:858	arg1	dogs					765:768	Twenty clinically healthy dogs	739:768	Twenty clinically healthy dogs	739:768	Twenty clinically healthy dogs were classified according to their body condition score (BCS) as obese (n = 10) or normal weight (n = 10).
35190784	4	75	theme	study	603:607	arg1	objective					585:593	The objective	581:593	The objective of this study	581:607	The objective of this study was to compare the composition and predicted function of the intestinal microbiome of Chilean obese and normal weight adult dogs.
35879158	3	0	contain	containing	773:782	arg2	ENZ					822:824	ENZ	822:824	ENZ	822:824	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	0	contain	containing	773:782	arg2	activities					810:819	amylolytic and fibrolytic activities	784:819	amylolytic and fibrolytic activities (ENZ)	784:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	0	contain	containing	773:782	arg1	treatments					613:622	1 of 2 treatments	606:622	1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	606:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	0	contain	containing	773:782	arg1	diet					682:685	the basal diet	672:685	the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	672:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	11	1	theme	milk	1607:1610	arg1	yields					1597:1602	greater yields	1589:1602	greater yields of milk, energy-corrected milk, milk true protein, and lactose	1589:1665	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	13	2	theme	glucose	1895:1901	arg1	Concentrations					1871:1884	Concentrations	1871:1884	Concentrations of blood glucose and total fatty acids	1871:1923	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	15	3	theme	primiparous	2267:2277	arg1	cows					2295:2298	primiparous and multiparous cows	2267:2298	primiparous and multiparous cows	2267:2298	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	6	4	theme	Milk	1047:1050	arg1	samples					1052:1058	Milk samples	1047:1058	Milk samples	1047:1058	Milk samples were collected every other week, and milk composition was averaged by week.
35879158	2	5	theme	enzyme	264:269	arg1	preparation					271:281	an exogenous enzyme preparation	251:281	an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger	251:327	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	15	6	theme	younger	2381:2387	arg1	animals					2389:2395	the younger animals	2377:2395	the younger animals	2377:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	13	7	theme	total	1907:1911	arg1	acids					1919:1923	total fatty acids	1907:1923	total fatty acids	1907:1923	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	9	8	theme	Milk	1423:1426	arg1	content					1432:1438	Milk fat content	1423:1438	Milk fat content	1423:1438	Milk fat content tended to be higher in CON cows.
35879158	14	9	from	concentrations	2133:2146	arg1	cows					2155:2158	all cows	2151:2158	all cows	2151:2158	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	14	10	theme	milk	2108:2111	arg1	protein					2113:2119	milk protein	2108:2119	milk protein	2108:2119	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	10	11	theme	treatment	1475:1483	arg1	interaction					1494:1504	A treatment × parity interaction	1473:1504	A treatment × parity interaction	1473:1504	A treatment × parity interaction was found for some of the production variables.
35879158	14	12	theme	primiparous	2184:2194	arg1	cows					2216:2219	primiparous but not multiparous cows	2184:2219	primiparous but not multiparous cows	2184:2219	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	6	13	theme	milk	1097:1100	arg1	composition					1102:1112	milk composition	1097:1112	milk composition	1097:1112	Milk samples were collected every other week, and milk composition was averaged by week.
35879158	11	14	theme	multiparous	1768:1778	arg1	cows					1780:1783	multiparous cows	1768:1783	multiparous cows	1768:1783	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	15	15	theme	ENZ	2354:2356	arg1	supplementation					2358:2372	ENZ supplementation	2354:2372	ENZ supplementation	2354:2372	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	4	16	with	premixes	859:866	arg1	control					899:905	control	899:905	control	899:905	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	4	16	with	premixes	859:866	arg1	preparation					884:894	the enzyme preparation	873:894	the enzyme preparation	873:894	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	4	17	theme	mixed	974:978	arg1	ration					980:985	total mixed ration	968:985	total mixed ration	968:985	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	3	18	theme	complete	536:543	arg1	experiment					558:567	a 10-wk randomized complete block design experiment	517:567	a 10-wk randomized complete block design experiment (total of 24 blocks)	517:588	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	9	19	theme	CON	1463:1465	arg1	cows					1467:1470	CON cows	1463:1470	CON cows	1463:1470	Milk fat content tended to be higher in CON cows.
35879158	11	20	theme	energy-corrected	1613:1628	arg1	milk					1630:1633	energy-corrected milk	1613:1633	energy-corrected milk	1613:1633	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	3	21	theme	10-wk	519:523	arg1	experiment					558:567	a 10-wk randomized complete block design experiment	517:567	a 10-wk randomized complete block design experiment (total of 24 blocks)	517:588	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	13	22	theme	ENZ	2027:2029	arg1	cows					2031:2034	ENZ cows	2027:2034	ENZ cows	2027:2034	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	7	23	theme	consecutive	1190:1200	arg1	days					1202:1205	2 consecutive days	1188:1205	2 consecutive days	1188:1205	Blood, fecal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment.
35879158	14	24	theme	lactose	2125:2131	arg1	concentrations					2133:2146	lactose concentrations	2125:2146	lactose concentrations	2125:2146	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	12	25	theme	nutrients	1826:1834	arg1	Intake					1786:1791	Intake	1786:1791	Intake	1786:1791	Intake and total-tract digestibility of nutrients did not differ between treatments.
35879158	12	25	theme	nutrients	1826:1834	arg1	total-tract					1797:1807	total-tract	1797:1807	total-tract	1797:1807	Intake and total-tract digestibility of nutrients did not differ between treatments.
35879158	11	26	theme	production	1709:1718	arg1	variables					1720:1728	these production variables	1703:1728	these production variables	1703:1728	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	4	27	theme	covariate	841:849	arg1	period					851:856	a 2-wk covariate period	834:856	a 2-wk covariate period	834:856	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	14	28	theme	enzyme	2060:2065	arg1	preparation					2067:2077	the exogenous enzyme preparation	2046:2077	the exogenous enzyme preparation used in this study	2046:2096	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	3	29	theme	basal	676:680	arg1	treatments					613:622	1 of 2 treatments	606:622	1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	606:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	29	theme	basal	676:680	arg1	diet					682:685	the basal diet	672:685	the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	672:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	30	theme	design	551:556	arg1	experiment					558:567	a 10-wk randomized complete block design experiment	517:567	a 10-wk randomized complete block design experiment (total of 24 blocks)	517:588	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	31	theme	exogenous	744:752	arg1	preparation					761:771	an exogenous enzyme preparation	741:771	an exogenous enzyme preparation	741:771	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	32	theme	dry	714:716	arg1	DMI					733:735	DMI	733:735	DMI	733:735	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	32	theme	dry	714:716	arg1	intake					725:730	4.2 g/kg dry matter intake	705:730	4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation	705:771	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	1	33	from	digestibility	138:150	arg1	cows					183:186	primiparous and multiparous cows	155:186	primiparous and multiparous cows	155:186	on lactational performance, metabolism, and digestibility in primiparous and multiparous cows.
35879158	0	34	theme	Aspergillus	77:87	arg1	spp					89:91	Aspergillus spp	77:91	Aspergillus spp	77:91	Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp.
35879158	10	35	theme	parity	1487:1492	arg1	interaction					1494:1504	A treatment × parity interaction	1473:1504	A treatment × parity interaction	1473:1504	A treatment × parity interaction was found for some of the production variables.
35879158	12	36	dep	Intake	1786:1791	arg1	digestibility					1809:1821	digestibility	1809:1821	digestibility	1809:1821	Intake and total-tract digestibility of nutrients did not differ between treatments.
35879158	3	37	theme	amylolytic	784:793	arg1	activities					810:819	amylolytic and fibrolytic activities	784:819	amylolytic and fibrolytic activities (ENZ)	784:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	37	theme	amylolytic	784:793	arg1	ENZ					822:824	ENZ	822:824	ENZ	822:824	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	15	38	from	result	2313:2318	arg1	DMI					2345:2347	DMI	2345:2347	DMI with ENZ supplementation in the younger animals	2345:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	11	39	theme	milk	1636:1639	arg1	protein					1646:1652	milk true protein	1636:1652	milk true protein	1636:1652	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	3	40	theme	preparation	761:771	arg1	DMI					733:735	DMI	733:735	DMI	733:735	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	40	theme	preparation	761:771	arg1	intake					725:730	4.2 g/kg dry matter intake	705:730	4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation	705:771	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	41	theme	Holstein	383:390	arg1	cows					392:395	Forty-eight Holstein cows	371:395	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk	371:498	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	10	42	theme	×	1485:1485	arg1	interaction					1494:1504	A treatment × parity interaction	1473:1504	A treatment × parity interaction	1473:1504	A treatment × parity interaction was found for some of the production variables.
35879158	11	43	theme	milk	1630:1633	arg1	yields					1597:1602	greater yields	1589:1602	greater yields of milk, energy-corrected milk, milk true protein, and lactose	1589:1665	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	14	44	from	production	2170:2179	arg1	cows					2216:2219	primiparous but not multiparous cows	2184:2219	primiparous but not multiparous cows	2184:2219	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	15	45	theme	production	2239:2248	arg1	result					2313:2318	a result	2311:2318	a result of a greater increase in DMI with ENZ supplementation in the younger animals	2311:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	15	45	theme	production	2239:2248	arg1	response					2250:2257	The differential production response	2222:2257	The differential production response between primiparous and multiparous cows	2222:2298	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	15	46	from	increase	2333:2340	arg1	DMI					2345:2347	DMI	2345:2347	DMI with ENZ supplementation in the younger animals	2345:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	3	47	theme	SD	446:447	arg1	yield					471:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	13	48	theme	β-hydroxybutyrate	1971:1987	arg1	concentration					1989:2001	β-hydroxybutyrate concentration	1971:2001	β-hydroxybutyrate concentration	1971:2001	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	3	49	theme	±	485:485	arg1	d					490:490	141 ± 52 d	481:490	141 ± 52 d	481:490	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	15	50	with	DMI	2345:2347	arg1	supplementation					2358:2372	ENZ supplementation	2354:2372	ENZ supplementation	2354:2372	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	3	51	theme	fibrolytic	799:808	arg1	activities					810:819	amylolytic and fibrolytic activities	784:819	amylolytic and fibrolytic activities (ENZ)	784:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	51	theme	fibrolytic	799:808	arg1	ENZ					822:824	ENZ	822:824	ENZ	822:824	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	52	theme	blocks	582:587	arg1	total					570:574	total	570:574	total	570:574	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	15	53	from	DMI	2345:2347	arg1	result					2313:2318	a result	2311:2318	a result of a greater increase in DMI with ENZ supplementation in the younger animals	2311:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	15	53	from	DMI	2345:2347	arg1	response					2250:2257	The differential production response	2222:2257	The differential production response between primiparous and multiparous cows	2222:2298	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	15	53	from	DMI	2345:2347	arg1	animals					2389:2395	the younger animals	2377:2395	the younger animals	2377:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	11	54	theme	protein	1646:1652	arg1	yields					1597:1602	greater yields	1589:1602	greater yields of milk, energy-corrected milk, milk true protein, and lactose	1589:1665	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	0	55	theme	enzyme	24:29	arg1	preparation					31:41	an exogenous enzyme preparation	11:41	an exogenous enzyme preparation	11:41	Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp.
35879158	7	56	theme	fecal	1143:1147	arg1	samples					1160:1166	Blood, fecal, and urine samples	1136:1166	Blood, fecal, and urine samples	1136:1166	Blood, fecal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment.
35879158	4	57	theme	enzyme	877:882	arg1	preparation					884:894	the enzyme preparation	873:894	the enzyme preparation	873:894	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	2	58	theme	lactational	332:342	arg1	performance					344:354	lactational performance	332:354	lactational performance of dairy cows	332:368	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	13	59	theme	ENZ	1946:1948	arg1	supplementation					1950:1964	ENZ supplementation	1946:1964	ENZ supplementation	1946:1964	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	7	60	theme	Blood	1136:1140	arg1	samples					1160:1166	Blood, fecal, and urine samples	1136:1166	Blood, fecal, and urine samples	1136:1166	Blood, fecal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment.
35879158	13	61	theme	fatty	1913:1917	arg1	acids					1919:1923	total fatty acids	1907:1923	total fatty acids	1907:1923	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	7	62	theme	experiment	1274:1283	arg1	week					1262:1265	the last week	1253:1265	the last week of the experiment	1253:1283	Blood, fecal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment.
35879158	8	63	theme	milk	1359:1362	arg1	concentrations					1364:1377	increased milk concentrations	1349:1377	increased milk concentrations of true protein, lactose, and other solids	1349:1420	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	2	64	theme	cows	365:368	arg1	performance					344:354	lactational performance	332:354	lactational performance of dairy cows	332:368	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	8	65	theme	solids	1415:1420	arg1	concentrations					1364:1377	increased milk concentrations	1349:1377	increased milk concentrations of true protein, lactose, and other solids	1349:1420	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	3	66	theme	milk	466:469	arg1	yield					471:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	13	67	theme	blood	1889:1893	arg1	glucose					1895:1901	blood glucose	1889:1901	blood glucose	1889:1901	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	7	68	theme	last	1257:1260	arg1	week					1262:1265	the last week	1253:1265	the last week of the experiment	1253:1283	Blood, fecal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment.
35879158	2	69	from	niger	323:327	arg1	preparation					271:281	an exogenous enzyme preparation	251:281	an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger	251:327	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	2	69	from	niger	323:327	arg1	effects					240:246	the effects	236:246	the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows	236:368	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	8	70	theme	protein	1387:1393	arg1	concentrations					1364:1377	increased milk concentrations	1349:1377	increased milk concentrations of true protein, lactose, and other solids	1349:1420	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	3	71	from	yield	471:475	arg1	milk					495:498	milk	495:498	milk	495:498	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	2	72	theme	preparation	271:281	arg1	effects					240:246	the effects	236:246	the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows	236:368	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	14	73	theme	multiparous	2204:2214	arg1	cows					2216:2219	primiparous but not multiparous cows	2184:2219	primiparous but not multiparous cows	2184:2219	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	14	74	from	protein	2113:2119	arg1	cows					2155:2158	all cows	2151:2158	all cows	2151:2158	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	8	75	theme	lactose	1396:1402	arg1	concentrations					1364:1377	increased milk concentrations	1349:1377	increased milk concentrations of true protein, lactose, and other solids	1349:1420	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	11	76	theme	greater	1589:1595	arg1	yields					1597:1602	greater yields	1589:1602	greater yields of milk, energy-corrected milk, milk true protein, and lactose	1589:1665	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	2	77	theme	exogenous	254:262	arg1	preparation					271:281	an exogenous enzyme preparation	251:281	an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger	251:327	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	1	78	theme	primiparous	155:165	arg1	cows					183:186	primiparous and multiparous cows	155:186	primiparous and multiparous cows	155:186	on lactational performance, metabolism, and digestibility in primiparous and multiparous cows.
35879158	9	79	theme	fat	1428:1430	arg1	content					1432:1438	Milk fat content	1423:1438	Milk fat content	1423:1438	Milk fat content tended to be higher in CON cows.
35879158	3	80	theme	basal	625:629	arg1	treatments					613:622	1 of 2 treatments	606:622	1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	606:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	80	theme	basal	625:629	arg1	diet					631:634	basal diet	625:634	basal diet	625:634	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	1	81	theme	multiparous	171:181	arg1	cows					183:186	primiparous and multiparous cows	155:186	primiparous and multiparous cows	155:186	on lactational performance, metabolism, and digestibility in primiparous and multiparous cows.
35879158	4	82	theme	total	968:972	arg1	ration					980:985	total mixed ration	968:985	total mixed ration	968:985	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	2	83	theme	Aspergillus	288:298	arg1	oryzae					300:305	Aspergillus oryzae	288:305	Aspergillus oryzae	288:305	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	15	84	theme	greater	2325:2331	arg1	increase					2333:2340	a greater increase	2323:2340	a greater increase in DMI with ENZ supplementation in the younger animals	2323:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	4	85	theme	ration	980:985	arg1	g					963:963	approximately 500 g	945:963	approximately 500 g of total mixed ration	945:985	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	3	86	theme	enzyme	640:645	arg1	supplementation					647:661	no enzyme supplementation	637:661	no enzyme supplementation (CON)	637:667	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	86	theme	enzyme	640:645	arg1	treatments					613:622	1 of 2 treatments	606:622	1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	606:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	86	theme	enzyme	640:645	arg1	CON					664:666	CON	664:666	CON	664:666	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	87	theme	randomized	525:534	arg1	experiment					558:567	a 10-wk randomized complete block design experiment	517:567	a 10-wk randomized complete block design experiment (total of 24 blocks)	517:588	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	88	dep	experiment	558:567	arg1	total					570:574	total	570:574	total	570:574	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	15	89	theme	increase	2333:2340	arg1	result					2313:2318	a result	2311:2318	a result of a greater increase in DMI with ENZ supplementation in the younger animals	2311:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	15	89	theme	increase	2333:2340	arg1	response					2250:2257	The differential production response	2222:2257	The differential production response between primiparous and multiparous cows	2222:2298	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	8	90	contain	had	1345:1347	arg1	cows					1305:1308	cows	1305:1308	cows fed ENZ	1305:1316	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	8	90	contain	had	1345:1347	arg2	concentrations					1364:1377	increased milk concentrations	1349:1377	increased milk concentrations of true protein, lactose, and other solids	1349:1420	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	2	91	theme	study	211:215	arg1	objective					193:201	The objective	189:201	The objective of this study	189:215	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	15	92	theme	differential	2226:2237	arg1	result					2313:2318	a result	2311:2318	a result of a greater increase in DMI with ENZ supplementation in the younger animals	2311:2395	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	15	92	theme	differential	2226:2237	arg1	response					2250:2257	The differential production response	2222:2257	The differential production response between primiparous and multiparous cows	2222:2298	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	1	93	theme	lactational	97:107	arg1	performance					109:119	lactational performance	97:119	lactational performance	97:119	on lactational performance, metabolism, and digestibility in primiparous and multiparous cows.
35879158	6	94	theme	other	1081:1085	arg1	week					1087:1090	every other week	1075:1090	every other week	1075:1090	Milk samples were collected every other week, and milk composition was averaged by week.
35879158	11	95	theme	Primiparous	1554:1564	arg1	cows					1566:1569	Primiparous cows	1554:1569	Primiparous cows receiving ENZ	1554:1583	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	0	96	theme	mixed	60:64	arg1	culture					66:72	a mixed culture	58:72	a mixed culture of Aspergillus spp	58:91	Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp.
35879158	3	97	theme	block	545:549	arg1	experiment					558:567	a 10-wk randomized complete block design experiment	517:567	a 10-wk randomized complete block design experiment (total of 24 blocks)	517:588	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	98	theme	±	455:455	arg1	yield					471:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	4	99	theme	2-wk	836:839	arg1	period					851:856	a 2-wk covariate period	834:856	a 2-wk covariate period	834:856	After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration.
35879158	14	100	theme	exogenous	2050:2058	arg1	preparation					2067:2077	the exogenous enzyme preparation	2046:2077	the exogenous enzyme preparation used in this study	2046:2096	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	5	101	theme	Production	988:997	arg1	data					999:1002	Production data	988:1002	Production data	988:1002	Production data were collected daily and averaged by week.
35879158	3	102	theme	enzyme	754:759	arg1	preparation					761:771	an exogenous enzyme preparation	741:771	an exogenous enzyme preparation	741:771	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	11	103	theme	primiparous	1685:1695	arg1	cows					1697:1700	CON primiparous cows	1681:1700	CON primiparous cows	1681:1700	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	3	104	from	d	490:490	arg1	milk					495:498	milk	495:498	milk	495:498	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	105	theme	matter	718:723	arg1	DMI					733:735	DMI	733:735	DMI	733:735	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	105	theme	matter	718:723	arg1	intake					725:730	4.2 g/kg dry matter intake	705:730	4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation	705:771	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	0	106	theme	spp	89:91	arg1	culture					66:72	a mixed culture	58:72	a mixed culture of Aspergillus spp	58:91	Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp.
35879158	2	107	from	oryzae	300:305	arg1	preparation					271:281	an exogenous enzyme preparation	251:281	an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger	251:327	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	2	107	from	oryzae	300:305	arg1	effects					240:246	the effects	236:246	the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows	236:368	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	3	108	dep	cows	392:395	arg1	primiparous					401:411	primiparous	401:411	primiparous	401:411	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	108	dep	cows	392:395	arg1	multiparous					420:430	multiparous	420:430	multiparous	420:430	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	109	theme	g/kg	709:712	arg1	DMI					733:735	DMI	733:735	DMI	733:735	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	109	theme	g/kg	709:712	arg1	intake					725:730	4.2 g/kg dry matter intake	705:730	4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation	705:771	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	15	110	theme	multiparous	2283:2293	arg1	cows					2295:2298	primiparous and multiparous cows	2267:2298	primiparous and multiparous cows	2267:2298	The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.
35879158	11	111	theme	true	1641:1644	arg1	protein					1646:1652	milk true protein	1636:1652	milk true protein	1636:1652	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	1	112	from	metabolism	122:131	arg1	cows					183:186	primiparous and multiparous cows	155:186	primiparous and multiparous cows	155:186	on lactational performance, metabolism, and digestibility in primiparous and multiparous cows.
35879158	7	113	theme	urine	1154:1158	arg1	samples					1160:1166	Blood, fecal, and urine samples	1136:1166	Blood, fecal, and urine samples	1136:1166	Blood, fecal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment.
35879158	0	114	theme	exogenous	14:22	arg1	preparation					31:41	an exogenous enzyme preparation	11:41	an exogenous enzyme preparation	11:41	Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp.
35879158	1	115	from	performance	109:119	arg1	cows					183:186	primiparous and multiparous cows	155:186	primiparous and multiparous cows	155:186	on lactational performance, metabolism, and digestibility in primiparous and multiparous cows.
35879158	11	116	theme	CON	1681:1683	arg1	cows					1697:1700	CON primiparous cows	1681:1700	CON primiparous cows	1681:1700	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	11	117	theme	lactose	1659:1665	arg1	yields					1597:1602	greater yields	1589:1602	greater yields of milk, energy-corrected milk, milk true protein, and lactose	1589:1665	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	0	118	theme	preparation	31:41	arg1	Effects					0:6	Effects	0:6	Effects of an exogenous enzyme preparation	0:41	Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp.
35879158	3	119	dep	treatments	613:622	arg1	supplementation					647:661	no enzyme supplementation	637:661	no enzyme supplementation (CON)	637:667	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	119	dep	treatments	613:622	arg1	treatments					613:622	1 of 2 treatments	606:622	1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	606:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	119	dep	treatments	613:622	arg1	diet					631:634	basal diet	625:634	basal diet	625:634	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	119	dep	treatments	613:622	arg1	diet					682:685	the basal diet	672:685	the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ)	672:825	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	3	119	dep	treatments	613:622	arg1	CON					664:666	CON	664:666	CON	664:666	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	2	120	from	effects	240:246	arg1	oryzae					300:305	Aspergillus oryzae	288:305	Aspergillus oryzae	288:305	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	2	120	from	effects	240:246	arg1	niger					323:327	Aspergillus niger	311:327	Aspergillus niger	311:327	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	2	120	from	effects	240:246	arg1	performance					344:354	lactational performance	332:354	lactational performance of dairy cows	332:368	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	3	121	theme	±	444:444	arg1	yield					471:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	11	122	contain	had	1585:1587	arg2	yields					1597:1602	greater yields	1589:1602	greater yields of milk, energy-corrected milk, milk true protein, and lactose	1589:1665	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	11	122	contain	had	1585:1587	arg1	cows					1566:1569	Primiparous cows	1554:1569	Primiparous cows receiving ENZ	1554:1583	Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows.
35879158	8	123	theme	increased	1349:1357	arg1	concentrations					1364:1377	increased milk concentrations	1349:1377	increased milk concentrations of true protein, lactose, and other solids	1349:1420	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	14	124	theme	milk	2165:2168	arg1	production					2170:2179	milk production	2165:2179	milk production in primiparous but not multiparous cows	2165:2219	Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows.
35879158	3	125	theme	kg/d	461:464	arg1	yield					471:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	(± SD) 36.3 ± 8.7 kg/d milk yield	443:475	Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ).
35879158	8	126	theme	other	1409:1413	arg1	solids					1415:1420	other solids	1409:1420	other solids	1409:1420	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	10	127	theme	production	1532:1541	arg1	variables					1543:1551	the production variables	1528:1551	the production variables	1528:1551	A treatment × parity interaction was found for some of the production variables.
35879158	2	128	theme	Aspergillus	311:321	arg1	niger					323:327	Aspergillus niger	311:327	Aspergillus niger	311:327	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35879158	13	129	theme	acids	1919:1923	arg1	Concentrations					1871:1884	Concentrations	1871:1884	Concentrations of blood glucose and total fatty acids	1871:1923	Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows.
35879158	8	130	theme	true	1382:1385	arg1	protein					1387:1393	true protein	1382:1393	true protein	1382:1393	Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids.
35879158	2	131	theme	dairy	359:363	arg1	cows					365:368	dairy cows	359:368	dairy cows	359:368	The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows.
35803504	7	0	theme	islet	1532:1536	arg1	transplantation					1538:1552	islet transplantation	1532:1552	islet transplantation	1532:1552	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	6	1	theme	designed	1098:1105	arg1	structure					1118:1126	the designed 3D-printed structure	1094:1126	the designed 3D-printed structure	1094:1126	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	6	1	theme	designed	1098:1105	arg1	Meanwhile					1083:1091	Meanwhile	1083:1091	Meanwhile	1083:1091	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	11	2	theme	designed	2171:2178	arg1	favorable					2184:2192	favorable	2184:2192	favorable	2184:2192	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	11	2	theme	designed	2171:2178	arg1	structures					2157:2166	the 3D-printed structures	2142:2166	the 3D-printed structures we designed	2142:2178	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	7	3	theme	clinical	1467:1474	arg1	applications					1476:1487	clinical applications	1467:1487	clinical applications	1467:1487	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	8	4	theme	vascular	1690:1697	arg1	system					1699:1704	vascular system	1690:1704	vascular system	1690:1704	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	10	5	theme	islet	2001:2005	arg1	adhesion					2012:2019	islet cell adhesion	2001:2019	islet cell adhesion	2001:2019	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	1	6	theme	diabetes	291:298	arg1	research					300:307	diabetes research	291:307	diabetes research	291:307	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	3	7	theme	islet	537:541	arg1	adhesion					548:555	islet cell adhesion	537:555	islet cell adhesion	537:555	The HAMA/pECM hydrogel was validated in vitro to maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, which helps improve islet function and activity.
35803504	6	8	theme	networks	1171:1178	arg1	formation					1149:1157	the formation	1145:1157	the formation of vascular networks	1145:1178	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	7	9	with	composition	1306:1316	arg1	effects					1389:1395	promising therapeutic effects	1367:1395	promising therapeutic effects	1367:1395	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	1	10	theme	extracellular	202:214	arg1	support					223:229	extracellular matrix support	202:229	extracellular matrix support	202:229	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	7	11	theme	HAMA	1415:1418	arg1	group					1429:1433	the HAMA hydrogel group	1411:1433	the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo	1411:1560	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	5	12	theme	vessels	1074:1080	arg1	density					1059:1065	the density	1055:1065	the density of new vessels	1055:1080	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	1	13	theme	support	223:229	arg1	lack					194:197	the lack	190:197	the lack of extracellular matrix support and neovascularization	190:252	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	4	14	theme	3D-printed	715:724	arg1	hydrogel					755:762	the 3D-printed islet-encapsulated HAMA/pECM hydrogel	711:762	the 3D-printed islet-encapsulated HAMA/pECM hydrogel	711:762	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	7	15	theme	hydrogel	1420:1427	arg1	group					1429:1433	the HAMA hydrogel group	1411:1433	the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo	1411:1560	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	10	16	theme	signaling	2063:2071	arg1	pathway					2073:2079	the Rac1/ROCK/MLCK signaling pathway	2044:2079	the Rac1/ROCK/MLCK signaling pathway	2044:2079	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	1	17	from	application	276:286	arg1	research					300:307	diabetes research	291:307	diabetes research	291:307	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	1	18	theme	neovascularization	235:252	arg1	lack					194:197	the lack	190:197	the lack of extracellular matrix support and neovascularization	190:252	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	9	19	theme	new	1774:1776	arg1	bioink					1794:1799	a new tissue-specific bioink	1772:1799	a new tissue-specific bioink	1772:1799	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	4	20	theme	islet-encapsulated	726:743	arg1	hydrogel					755:762	the 3D-printed islet-encapsulated HAMA/pECM hydrogel	711:762	the 3D-printed islet-encapsulated HAMA/pECM hydrogel	711:762	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	11	21	from	favorable	2184:2192	arg1	addition					2132:2139	addition	2132:2139	addition	2132:2139	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	3	22	theme	signaling	599:607	arg1	pathway					609:615	the Rac1/ROCK/MLCK signaling pathway	580:615	the Rac1/ROCK/MLCK signaling pathway	580:615	The HAMA/pECM hydrogel was validated in vitro to maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, which helps improve islet function and activity.
35803504	4	23	theme	glucose	923:929	arg1	stimulation					931:941	blood glucose stimulation	917:941	blood glucose stimulation	917:941	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	6	24	theme	islet	1227:1231	arg1	organoids					1233:1241	3D-printed islet organoids	1216:1241	3D-printed islet organoids	1216:1241	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	3	25	theme	islet	638:642	arg1	function					644:651	islet function	638:651	islet function	638:651	The HAMA/pECM hydrogel was validated in vitro to maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, which helps improve islet function and activity.
35803504	1	26	theme	poor	155:158	arg1	efficacy					170:177	poor long-term efficacy	155:177	poor long-term efficacy	155:177	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	7	27	theme	therapeutic	1377:1387	arg1	effects					1389:1395	promising therapeutic effects	1367:1395	promising therapeutic effects	1367:1395	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	0	28	theme	3D	76:77	arg1	bioink					88:93	a potential 3D printing bioink	64:93	a potential 3D printing bioink for constructing islet organoids	64:126	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	0	28	theme	3D	76:77	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix	40:59	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	9	29	theme	extracellular	1825:1837	arg1	pECM					1847:1850	pECM	1847:1850	pECM	1847:1850	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	9	29	theme	extracellular	1825:1837	arg1	matrix					1839:1844	pancreatic extracellular matrix	1814:1844	pancreatic extracellular matrix (pECM)	1814:1851	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	11	30	theme	3D-printed	2146:2155	arg1	favorable					2184:2192	favorable	2184:2192	favorable	2184:2192	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	11	30	theme	3D-printed	2146:2155	arg1	structures					2157:2166	the 3D-printed structures	2142:2166	the 3D-printed structures we designed	2142:2178	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	11	31	from	addition	2132:2139	arg1	favorable					2184:2192	favorable	2184:2192	favorable	2184:2192	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	11	31	from	addition	2132:2139	arg1	structures					2157:2166	the 3D-printed structures	2142:2166	the 3D-printed structures we designed	2142:2178	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	11	32	theme	transplantation	2292:2306	arg1	efficacy					2274:2281	the long-term efficacy	2260:2281	the long-term efficacy of islet transplantation	2260:2306	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	7	33	dep	composition	1306:1316	arg1	the					1285:1287	the	1285:1287	the	1285:1287	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	7	34	theme	promising	1367:1375	arg1	effects					1389:1395	promising therapeutic effects	1367:1395	promising therapeutic effects	1367:1395	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	9	35	theme	hyaluronic	1857:1866	arg1	acid					1868:1871	hyaluronic acid	1857:1871	hyaluronic acid methacrylate (HAMA)	1857:1891	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	8	36	theme	SIGNIFICANCE	1576:1587	arg1	STATEMENT					1563:1571	STATEMENT	1563:1571	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.	1563:1757	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	4	37	theme	insulin	774:780	arg1	levels					782:787	insulin levels	774:787	insulin levels	774:787	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	0	38	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	7	39	theme	organoids	1352:1360	arg1	structure					1333:1341	3D-printed structure	1322:1341	3D-printed structure	1322:1341	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	7	39	theme	organoids	1352:1360	arg1	composition					1306:1316	HAMA/pECM bioink composition	1289:1316	HAMA/pECM bioink composition	1289:1316	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	11	40	theme	blood	2219:2223	arg1	networks					2232:2239	new blood vessel networks	2215:2239	new blood vessel networks	2215:2239	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	2	41	theme	acid	433:436	arg1	HAMA					452:455	HAMA	452:455	HAMA	452:455	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	41	theme	acid	433:436	arg1	methacrylate					438:449	hyaluronic acid methacrylate	422:449	hyaluronic acid methacrylate (HAMA)	422:456	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	41	theme	acid	433:436	arg1	bioinks					470:476	specific bioinks	461:476	specific bioinks	461:476	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	41	theme	acid	433:436	arg1	matrix					404:409	a pancreatic extracellular matrix	377:409	a pancreatic extracellular matrix (pECM)	377:416	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	4	42	theme	normal	847:852	arg1	range					854:858	a normal range	845:858	a normal range for 90 days	845:870	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	5	43	theme	vessels	1034:1040	arg1	growth					1014:1019	growth	1014:1019	growth	1014:1019	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	5	43	theme	vessels	1034:1040	arg1	attachment					999:1008	attachment	999:1008	attachment	999:1008	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	7	44	theme	3D-printed	1322:1331	arg1	structure					1333:1341	3D-printed structure	1322:1341	3D-printed structure	1322:1341	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	2	45	theme	specific	461:468	arg1	methacrylate					438:449	hyaluronic acid methacrylate	422:449	hyaluronic acid methacrylate (HAMA)	422:456	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	45	theme	specific	461:468	arg1	bioinks					470:476	specific bioinks	461:476	specific bioinks	461:476	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	45	theme	specific	461:468	arg1	matrix					404:409	a pancreatic extracellular matrix	377:409	a pancreatic extracellular matrix (pECM)	377:416	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	8	46	theme	pancreatic	1616:1625	arg1	islets					1627:1632	pancreatic islets	1616:1632	pancreatic islets	1616:1632	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	10	47	theme	islet	1898:1902	arg1	organoids					1904:1912	The islet organoids	1894:1912	The islet organoids constructed by 3D printing	1894:1939	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	11	48	theme	islet	2286:2290	arg1	transplantation					2292:2306	islet transplantation	2286:2306	islet transplantation	2286:2306	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	4	49	theme	glucose	823:829	arg1	levels					831:836	blood glucose levels	817:836	blood glucose levels	817:836	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	2	50	theme	3D-printed	338:347	arg1	organoid					355:362	a 3D-printed islet organoid	336:362	a 3D-printed islet organoid	336:362	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	7	51	theme	transplantation	1538:1552	arg1	safety					1522:1527	safety	1522:1527	safety	1522:1527	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	7	51	theme	transplantation	1538:1552	arg1	effectiveness					1504:1516	effectiveness	1504:1516	effectiveness	1504:1516	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	4	52	dep	in	676:677	arg1	vivo					679:682	vivo	679:682	vivo	679:682	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	7	53	theme	HAMA/pECM	1289:1297	arg1	composition					1306:1316	HAMA/pECM bioink composition	1289:1316	HAMA/pECM bioink composition	1289:1316	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	10	54	theme	3D	1929:1930	arg1	printing					1932:1939	3D printing	1929:1939	3D printing	1929:1939	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	2	55	theme	pancreatic	379:388	arg1	methacrylate					438:449	hyaluronic acid methacrylate	422:449	hyaluronic acid methacrylate (HAMA)	422:456	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	55	theme	pancreatic	379:388	arg1	pECM					412:415	pECM	412:415	pECM	412:415	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	55	theme	pancreatic	379:388	arg1	bioinks					470:476	specific bioinks	461:476	specific bioinks	461:476	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	55	theme	pancreatic	379:388	arg1	matrix					404:409	a pancreatic extracellular matrix	377:409	a pancreatic extracellular matrix (pECM)	377:416	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	5	56	theme	new	1024:1026	arg1	vessels					1034:1040	new blood vessels	1024:1040	new blood vessels	1024:1040	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	6	57	theme	3D-printed	1107:1116	arg1	structure					1118:1126	the designed 3D-printed structure	1094:1126	the designed 3D-printed structure	1094:1126	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	6	57	theme	3D-printed	1107:1116	arg1	Meanwhile					1083:1091	Meanwhile	1083:1091	Meanwhile	1083:1091	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	1	58	contain	has	151:153	arg2	efficacy					170:177	poor long-term efficacy	155:177	poor long-term efficacy	155:177	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	1	58	contain	has	151:153	arg1	transplantation					135:149	Islet transplantation	129:149	Islet transplantation	129:149	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	8	59	theme	poor	1720:1723	arg1	efficiency					1747:1756	poor islet transplantation efficiency	1720:1756	poor islet transplantation efficiency	1720:1756	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	1	60	theme	wide	271:274	arg1	application					276:286	its wide application	267:286	its wide application in diabetes research	267:307	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	8	61	theme	extracellular	1665:1677	arg1	matrix					1679:1684	the extracellular matrix	1661:1684	the extracellular matrix	1661:1684	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	3	62	theme	cell	543:546	arg1	adhesion					548:555	islet cell adhesion	537:555	islet cell adhesion	537:555	The HAMA/pECM hydrogel was validated in vitro to maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, which helps improve islet function and activity.
35803504	8	63	theme	transplantation	1731:1745	arg1	efficiency					1747:1756	poor islet transplantation efficiency	1720:1756	poor islet transplantation efficiency	1720:1756	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	11	64	theme	new	2215:2217	arg1	networks					2232:2239	new blood vessel networks	2215:2239	new blood vessel networks	2215:2239	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	7	65	used	used	1459:1462	arg2	group					1429:1433	the HAMA hydrogel group	1411:1433	the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo	1411:1560	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	11	66	dep	designed	2171:2178	arg1	we					2168:2169	we	2168:2169	we	2168:2169	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	10	67	theme	pancreas	1979:1986	arg1	microenvironment					1955:1970	the microenvironment	1951:1970	the microenvironment of the pancreas	1951:1986	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	6	68	theme	vascular	1162:1169	arg1	networks					1171:1178	vascular networks	1162:1178	vascular networks	1162:1178	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	4	69	theme	in	676:677	arg1	experiments					684:694	in vivo experiments	676:694	in vivo experiments	676:694	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	1	70	theme	matrix	216:221	arg1	support					223:229	extracellular matrix support	202:229	extracellular matrix support	202:229	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	10	71	theme	cell	2007:2010	arg1	adhesion					2012:2019	islet cell adhesion	2001:2019	islet cell adhesion	2001:2019	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	7	72	with	structure	1333:1341	arg1	effects					1389:1395	promising therapeutic effects	1367:1395	promising therapeutic effects	1367:1395	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	10	73	theme	Rac1/ROCK/MLCK	2048:2061	arg1	pathway					2073:2079	the Rac1/ROCK/MLCK signaling pathway	2044:2079	the Rac1/ROCK/MLCK signaling pathway	2044:2079	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	3	74	theme	Rac1/ROCK/MLCK	584:597	arg1	pathway					609:615	the Rac1/ROCK/MLCK signaling pathway	580:615	the Rac1/ROCK/MLCK signaling pathway	580:615	The HAMA/pECM hydrogel was validated in vitro to maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, which helps improve islet function and activity.
35803504	1	75	theme	Islet	129:133	arg1	transplantation					135:149	Islet transplantation	129:149	Islet transplantation	129:149	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	9	76	theme	tissue-specific	1778:1792	arg1	bioink					1794:1799	a new tissue-specific bioink	1772:1799	a new tissue-specific bioink	1772:1799	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	6	77	theme	organoids	1233:1241	arg1	construction					1200:1211	the construction	1196:1211	the construction of 3D-printed islet organoids	1196:1241	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	5	78	dep	attachment	999:1008	arg1	the					995:997	the	995:997	the	995:997	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	4	79	theme	blood	917:921	arg1	stimulation					931:941	blood glucose stimulation	917:941	blood glucose stimulation	917:941	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	6	80	theme	3D-printed	1216:1225	arg1	organoids					1233:1241	3D-printed islet organoids	1216:1241	3D-printed islet organoids	1216:1241	Meanwhile, the designed 3D-printed structure was conducive to the formation of vascular networks and it promoted the construction of 3D-printed islet organoids.
35803504	4	81	theme	HAMA/pECM	745:753	arg1	hydrogel					755:762	the 3D-printed islet-encapsulated HAMA/pECM hydrogel	711:762	the 3D-printed islet-encapsulated HAMA/pECM hydrogel	711:762	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	0	82	theme	printing	79:86	arg1	bioink					88:93	a potential 3D printing bioink	64:93	a potential 3D printing bioink for constructing islet organoids	64:126	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	0	82	theme	printing	79:86	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix	40:59	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	5	83	theme	new	1070:1072	arg1	vessels					1074:1080	new vessels	1070:1080	new vessels	1070:1080	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	1	84	theme	long-term	160:168	arg1	efficacy					170:177	poor long-term efficacy	155:177	poor long-term efficacy	155:177	Islet transplantation has poor long-term efficacy because of the lack of extracellular matrix support and neovascularization; this limits its wide application in diabetes research.
35803504	9	85	theme	pancreatic	1814:1823	arg1	pECM					1847:1850	pECM	1847:1850	pECM	1847:1850	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	9	85	theme	pancreatic	1814:1823	arg1	matrix					1839:1844	pancreatic extracellular matrix	1814:1844	pancreatic extracellular matrix (pECM)	1814:1851	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	11	86	theme	long-term	2264:2272	arg1	efficacy					2274:2281	the long-term efficacy	2260:2281	the long-term efficacy of islet transplantation	2260:2306	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	0	87	theme	islet	112:116	arg1	organoids					118:126	islet organoids	112:126	islet organoids	112:126	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	4	88	theme	diabetic	792:799	arg1	mice					801:804	diabetic mice	792:804	diabetic mice	792:804	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	10	89	theme	islet	2100:2104	arg1	function					2106:2113	islet function	2100:2113	islet function	2100:2113	The islet organoids constructed by 3D printing can mimic the microenvironment of the pancreas and maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, thereby improving islet function and activity.
35803504	7	90	theme	islet	1346:1350	arg1	organoids					1352:1360	islet organoids	1346:1360	islet organoids	1346:1360	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	9	91	theme	acid	1868:1871	arg1	methacrylate					1873:1884	hyaluronic acid methacrylate	1857:1884	hyaluronic acid methacrylate (HAMA)	1857:1891	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	9	91	theme	acid	1868:1871	arg1	HAMA					1887:1890	HAMA	1887:1890	HAMA	1887:1890	We developed a new tissue-specific bioink by combining pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA).
35803504	8	92	theme	extraction	1594:1603	arg1	process					1605:1611	The extraction process	1590:1611	The extraction process of pancreatic islets	1590:1632	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	2	93	theme	extracellular	390:402	arg1	methacrylate					438:449	hyaluronic acid methacrylate	422:449	hyaluronic acid methacrylate (HAMA)	422:456	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	93	theme	extracellular	390:402	arg1	pECM					412:415	pECM	412:415	pECM	412:415	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	93	theme	extracellular	390:402	arg1	bioinks					470:476	specific bioinks	461:476	specific bioinks	461:476	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	2	93	theme	extracellular	390:402	arg1	matrix					404:409	a pancreatic extracellular matrix	377:409	a pancreatic extracellular matrix (pECM)	377:416	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	0	94	theme	extracellular	40:52	arg1	bioink					88:93	a potential 3D printing bioink	64:93	a potential 3D printing bioink for constructing islet organoids	64:126	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	0	94	theme	extracellular	40:52	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix	40:59	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	5	95	theme	HAMA/pECM	961:969	arg1	hydrogel					971:978	the HAMA/pECM hydrogel	957:978	the HAMA/pECM hydrogel	957:978	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	11	96	theme	vessel	2225:2230	arg1	networks					2232:2239	new blood vessel networks	2215:2239	new blood vessel networks	2215:2239	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	2	97	theme	hyaluronic	422:431	arg1	acid					433:436	hyaluronic acid	422:436	hyaluronic acid methacrylate (HAMA)	422:456	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	5	98	theme	blood	1028:1032	arg1	vessels					1034:1040	new blood vessels	1024:1040	new blood vessels	1024:1040	In addition, the HAMA/pECM hydrogel can facilitate the attachment and growth of new blood vessels and increase the density of new vessels.
35803504	8	99	theme	islets	1627:1632	arg1	process					1605:1611	The extraction process	1590:1611	The extraction process of pancreatic islets	1590:1632	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	0	100	theme	potential	66:74	arg1	bioink					88:93	a potential 3D printing bioink	64:93	a potential 3D printing bioink for constructing islet organoids	64:126	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	0	100	theme	potential	66:74	arg1	matrix					54:59	extracellular matrix	40:59	extracellular matrix	40:59	Hyaluronic acid methacrylate/pancreatic extracellular matrix as a potential 3D printing bioink for constructing islet organoids.
35803504	7	101	theme	bioink	1299:1304	arg1	composition					1306:1316	HAMA/pECM bioink composition	1289:1316	HAMA/pECM bioink composition	1289:1316	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	2	102	theme	islet	349:353	arg1	organoid					355:362	a 3D-printed islet organoid	336:362	a 3D-printed islet organoid	336:362	In this study, we develop a 3D-printed islet organoid by combining a pancreatic extracellular matrix (pECM) and hyaluronic acid methacrylate (HAMA) as specific bioinks.
35803504	7	103	dep	effectiveness	1504:1516	arg1	the					1500:1502	the	1500:1502	the	1500:1502	In conclusion, our experiments optimized the HAMA/pECM bioink composition and 3D-printed structure of islet organoids with promising therapeutic effects compared with the HAMA hydrogel group that can be potentially used in clinical applications to improve the effectiveness and safety of islet transplantation in vivo.
35803504	4	104	theme	blood	817:821	arg1	levels					831:836	blood glucose levels	817:836	blood glucose levels	817:836	Further, in vivo experiments confirmed that the 3D-printed islet-encapsulated HAMA/pECM hydrogel increases insulin levels in diabetic mice, maintains blood glucose levels within a normal range for 90 days, and rapidly secretes insulin in response to blood glucose stimulation.
35803504	3	105	theme	HAMA/pECM	483:491	arg1	hydrogel					493:500	The HAMA/pECM hydrogel	479:500	The HAMA/pECM hydrogel	479:500	The HAMA/pECM hydrogel was validated in vitro to maintain islet cell adhesion and morphology through the Rac1/ROCK/MLCK signaling pathway, which helps improve islet function and activity.
35803504	11	106	theme	networks	2232:2239	arg1	formation					2202:2210	the formation	2198:2210	the formation of new blood vessel networks	2198:2239	In addition, the 3D-printed structures we designed are favorable for the formation of new blood vessel networks, bringing hope for the long-term efficacy of islet transplantation.
35803504	8	107	theme	islet	1725:1729	arg1	efficiency					1747:1756	poor islet transplantation efficiency	1720:1756	poor islet transplantation efficiency	1720:1756	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
35803504	8	108	dep	STATEMENT	1563:1571	arg1	cause					1645:1649	cause	1645:1649	cause	1645:1649	STATEMENT OF SIGNIFICANCE: The extraction process of pancreatic islets can easily cause damage to the extracellular matrix and vascular system, resulting in poor islet transplantation efficiency.
36144586	9	0	theme	radical	1556:1562	arg1	activity					1575:1582	the best radical scavenging activity	1547:1582	the best radical scavenging activity	1547:1582	The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL.
36144586	11	1	theme	63.75	1854:1858	arg1	%					1859:1859	%	1859:1859	%	1859:1859	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	10	2	theme	antidiabetic	1626:1637	arg1	effect					1639:1644	antidiabetic effect	1626:1644	antidiabetic effect	1626:1644	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	6	3	theme	test	1176:1179	arg1	inhibition					1145:1154	the inhibition	1141:1154	the inhibition of the lipoxygenase test	1141:1179	The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test.
36144586	10	4	theme	best	1664:1667	arg1	values					1669:1674	the best values	1660:1674	the best values	1660:1674	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	5	5	theme	free	909:912	arg1	scavenging					922:931	the free radical scavenging	905:931	the free radical scavenging ability (DPPH method)	905:953	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	12	6	theme	studied	1914:1920	arg1	species					1922:1928	the studied species	1910:1928	the studied species	1910:1928	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	3	7	theme	different	597:605	arg1	strains					618:624	different pathogenic strains	597:624	different pathogenic strains of microorganisms	597:642	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	13	8	theme	bioactive	2355:2363	arg1	molecules					2365:2373	their bioactive molecules	2349:2373	their bioactive molecules	2349:2373	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	9	9	theme	533.89	1601:1606	arg1	µg/mL					1616:1620	533.89 ± 15.05 µg/mL	1601:1620	533.89 ± 15.05 µg/mL	1601:1620	The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL.
36144586	13	10	theme	drug	2238:2241	arg1	target					2243:2248	a potential drug target	2226:2248	a potential drug target against diabetes, inflammation and microbial infections	2226:2304	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	13	10	theme	drug	2238:2241	arg1	EOs					2196:2198	the EOs	2192:2198	the EOs of M. chamomilla	2192:2215	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	11	11	theme	mg/kg	1800:1804	arg1	dose					1788:1791	a dose	1786:1791	a dose of 100 mg/kg	1786:1804	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	12	12	dep	antimicrobial	1950:1962	arg1	effect					2118:2123	effect	2118:2123	an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains	1937:2154	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	12	dep	antimicrobial	1950:1962	arg1	mm					2012:2013	22.97 ± 0.16 mm	1999:2013	22.97 ± 0.16 mm	1999:2013	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	12	dep	antimicrobial	1950:1962	arg1	29213					1992:1996	Staphylococcus aureus ATCC 29213	1965:1996	Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)	1965:2014	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	12	dep	antimicrobial	1950:1962	arg1	albicans					2091:2098	Candida albicans	2083:2098	an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains	1937:2154	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	9	13	theme	15.05	1610:1614	arg1	µg/mL					1616:1620	533.89 ± 15.05 µg/mL	1601:1620	533.89 ± 15.05 µg/mL	1601:1620	The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL.
36144586	12	14	theme	±	2107:2107	arg1	mm					2114:2115	21.07 ± 0.24 mm	2101:2115	21.07 ± 0.24 mm	2101:2115	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	14	theme	±	2107:2107	arg1	antimicrobial					1950:1962	antimicrobial	1950:1962	antimicrobial	1950:1962	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	3	15	theme	microorganisms	629:642	arg1	strains					618:624	different pathogenic strains	597:624	different pathogenic strains of microorganisms	597:642	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	13	16	dep	investigations	2324:2337	arg1	assess					2342:2347	assess	2342:2347	to assess their bioactive molecules individually and in combination	2339:2405	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	11	17	theme	anti-inflammatory	1823:1839	arg1	effect					1841:1846	a high anti-inflammatory effect	1816:1846	a high anti-inflammatory effect	1816:1846	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	6	18	theme	anti-inflammatory	1029:1045	arg1	effects					1047:1053	The anti-inflammatory effects	1025:1053	The anti-inflammatory effects	1025:1053	The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test.
36144586	12	19	theme	Candida	2083:2089	arg1	albicans					2091:2098	Candida albicans	2083:2098	an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains	1937:2154	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	0	20	theme	Properties	68:77	arg1	Investigation					40:52	Investigation	40:52	Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture	40:143	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	0	20	theme	Properties	68:77	arg1	Determination					0:12	Determination	0:12	Determination of Chemical Compounds	0:34	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	10	21	theme	±	1706:1706	arg1	μg/mL					1713:1717	265.57 ± 0.03 μg/mL	1699:1717	265.57 ± 0.03 μg/mL	1699:1717	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	10	21	theme	±	1706:1706	arg1	α-glucosidase					1684:1696	α-glucosidase	1684:1696	α-glucosidase (265.57 ± 0.03 μg/mL)	1684:1718	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	5	22	theme	scavenging	922:931	arg1	ability					933:939	the free radical scavenging ability	905:939	the free radical scavenging ability (DPPH method)	905:953	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	5	22	theme	scavenging	922:931	arg1	method					947:952	DPPH method	942:952	DPPH method	942:952	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	2	23	theme	chromatography-mass	501:519	arg1	spectrophotometry					521:537	gas chromatography-mass spectrophotometry	497:537	gas chromatography-mass spectrophotometry (GC-MS)	497:545	EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography-mass spectrophotometry (GC-MS).
36144586	2	23	theme	chromatography-mass	501:519	arg1	GC-MS					540:544	GC-MS	540:544	GC-MS	540:544	EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography-mass spectrophotometry (GC-MS).
36144586	8	24	theme	antioxidant	1414:1424	arg1	activity					1426:1433	antioxidant activity	1414:1433	antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL)	1414:1522	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	0	25	theme	Matricaria	82:91	arg1	Oils					114:117	Matricaria chamomilla Essential Oils	82:117	Matricaria chamomilla Essential Oils	82:117	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	12	26	dep	antifungal	2018:2027	arg1	mm					2062:2063	18.13 ± 0.18 mm	2049:2063	18.13 ± 0.18 mm	2049:2063	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	26	dep	antifungal	2018:2027	arg1	niger					2042:2046	Aspergillus niger	2030:2046	Aspergillus niger (18.13 ± 0.18 mm)	2030:2064	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	3	27	theme	inhibitory	687:696	arg1	MIC					713:715	MIC	713:715	MIC	713:715	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	3	27	theme	inhibitory	687:696	arg1	concentration					698:710	the minimum inhibitory concentration	675:710	the minimum inhibitory concentration (MIC)	675:716	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	0	28	theme	Essential	104:112	arg1	Oils					114:117	Matricaria chamomilla Essential Oils	82:117	Matricaria chamomilla Essential Oils	82:117	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	1	29	theme	essential	374:382	arg1	oils					384:387	Matricaria chamomilla L. essential oils	349:387	Matricaria chamomilla L. essential oils (EOs)	349:393	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	1	29	theme	essential	374:382	arg1	EOs					390:392	EOs	390:392	EOs	390:392	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	8	30	theme	DPPH	1447:1450	arg1	assay					1452:1456	the DPPH assay	1443:1456	the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL)	1443:1522	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	7	31	theme	phytochemical	1202:1214	arg1	composition					1216:1226	the phytochemical composition	1198:1226	the phytochemical composition	1198:1226	The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), β-myrcene (8.01%), and chamazulene (6.54%).
36144586	6	32	theme	carrageenan-induced	1085:1103	arg1	method					1115:1120	the carrageenan-induced paw edema method	1081:1120	the carrageenan-induced paw edema method	1081:1120	The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test.
36144586	2	33	theme	phytochemical	456:468	arg1	screening					470:478	phytochemical screening	456:478	phytochemical screening	456:478	EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography-mass spectrophotometry (GC-MS).
36144586	12	34	theme	±	2055:2055	arg1	mm					2062:2063	18.13 ± 0.18 mm	2049:2063	18.13 ± 0.18 mm	2049:2063	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	34	theme	±	2055:2055	arg1	niger					2042:2046	Aspergillus niger	2030:2046	Aspergillus niger (18.13 ± 0.18 mm)	2030:2064	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	1	35	theme	anti-inflammatory	283:299	arg1	activities					334:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	5	36	theme	ion	964:966	arg1	chelating					968:976	ferrous ion chelating	956:976	ferrous ion chelating (FIC) ability	956:990	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	5	36	theme	ion	964:966	arg1	FIC					979:981	FIC	979:981	FIC	979:981	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	0	37	theme	Chemical	17:24	arg1	Compounds					26:34	Chemical Compounds	17:34	Chemical Compounds	17:34	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	1	38	theme	antidiabetic	302:313	arg1	activities					334:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	11	39	theme	honey	1769:1773	arg1	mixture					1775:1781	honey mixture	1769:1781	honey mixture	1769:1781	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	12	40	theme	22.97	1999:2003	arg1	±					2005:2005	±	2005:2005	±	2005:2005	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	2	41	theme	chamomilla	406:415	arg1	EOs					396:398	EOs	396:398	EOs of M. chamomilla	396:415	EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography-mass spectrophotometry (GC-MS).
36144586	7	42	theme	EOs	1294:1296	arg1	camphor					1251:1257	camphor	1251:1257	camphor (16.42%)	1251:1266	The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), β-myrcene (8.01%), and chamazulene (6.54%).
36144586	7	42	theme	EOs	1294:1296	arg1	compound					1282:1289	the major compound	1272:1289	the major compound of EOs	1272:1296	The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), β-myrcene (8.01%), and chamazulene (6.54%).
36144586	8	43	theme	15.05	1486:1490	arg1	µg/mL					1492:1496	533.89 ± 15.05 µg/mL	1477:1496	533.89 ± 15.05 µg/mL	1477:1496	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	3	44	theme	minimum	679:685	arg1	MIC					713:715	MIC	713:715	MIC	713:715	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	3	44	theme	minimum	679:685	arg1	concentration					698:710	the minimum inhibitory concentration	675:710	the minimum inhibitory concentration (MIC)	675:716	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	12	45	theme	0.16	2007:2010	arg1	±					2005:2005	±	2005:2005	±	2005:2005	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	13	46	theme	further	2316:2322	arg1	investigations					2324:2337	further investigations	2316:2337	further investigations to assess their bioactive molecules individually and in combination	2316:2405	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	3	47	theme	diffusion	658:666	arg1	assay					668:672	disc diffusion assay	653:672	disc diffusion assay	653:672	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	12	48	theme	aureus	1980:1985	arg1	mm					2012:2013	22.97 ± 0.16 mm	1999:2013	22.97 ± 0.16 mm	1999:2013	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	48	theme	aureus	1980:1985	arg1	29213					1992:1996	Staphylococcus aureus ATCC 29213	1965:1996	Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)	1965:2014	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	5	49	theme	antioxidant	860:870	arg1	activity					872:879	The antioxidant activity	856:879	The antioxidant activity of EOs	856:886	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	8	50	theme	±	1509:1509	arg1	µg/mL					1517:1521	1945.38 ± 12.71 µg/mL	1501:1521	1945.38 ± 12.71 µg/mL	1501:1521	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	10	51	theme	±	1742:1742	arg1	μg/mL					1749:1753	121.44 ± 0.05 μg/mL	1735:1753	121.44 ± 0.05 μg/mL	1735:1753	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	10	51	theme	±	1742:1742	arg1	α-amylase					1724:1732	α-amylase	1724:1732	α-amylase (121.44 ± 0.05 μg/mL)	1724:1754	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	4	52	theme	inhibition	839:848	arg1	test					850:853	the enzyme inhibition test	828:853	the enzyme inhibition test	828:853	The antidiabetic activity was performed in vitro using the enzyme inhibition test.
36144586	8	53	dep	assay	1452:1456	arg1	IC50					1459:1462	IC50	1459:1462	IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL	1459:1521	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	12	54	theme	tested	2141:2146	arg1	strains					2148:2154	all the tested strains	2133:2154	all the tested strains	2133:2154	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	9	55	theme	best	1551:1554	arg1	activity					1575:1582	the best radical scavenging activity	1547:1582	the best radical scavenging activity	1547:1582	The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL.
36144586	6	56	theme	edema	1109:1113	arg1	method					1115:1120	the carrageenan-induced paw edema method	1081:1120	the carrageenan-induced paw edema method	1081:1120	The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test.
36144586	3	57	theme	minimum	723:729	arg1	MBC					759:761	MBC	759:761	MBC	759:761	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	3	57	theme	minimum	723:729	arg1	concentration					744:756	minimum bactericidal concentration	723:756	minimum bactericidal concentration (MBC)	723:762	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	13	58	theme	microbial	2285:2293	arg1	infections					2295:2304	microbial infections	2285:2304	microbial infections	2285:2304	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	5	59	theme	β-carotene	997:1006	arg1	assay					1018:1022	β-carotene bleaching assay	997:1022	β-carotene bleaching assay	997:1022	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	1	60	theme	antioxidant	270:280	arg1	activities					334:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	9	61	theme	scavenging	1564:1573	arg1	activity					1575:1582	the best radical scavenging activity	1547:1582	the best radical scavenging activity	1547:1582	The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL.
36144586	3	62	theme	antimicrobial	552:564	arg1	activities					566:575	The antimicrobial activities	548:575	The antimicrobial activities	548:575	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	3	63	theme	pathogenic	607:616	arg1	strains					618:624	different pathogenic strains	597:624	different pathogenic strains of microorganisms	597:642	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	6	64	theme	lipoxygenase	1163:1174	arg1	test					1176:1179	the lipoxygenase test	1159:1179	the lipoxygenase test	1159:1179	The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test.
36144586	11	65	from	dose	1788:1791	arg1	EOs					1761:1763	EOs	1761:1763	EOs	1761:1763	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	11	65	from	dose	1788:1791	arg1	mixture					1775:1781	honey mixture	1769:1781	honey mixture	1769:1781	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	11	66	theme	%	1859:1859	arg1	inhibition					1867:1876	63.75% edema inhibition	1854:1876	63.75% edema inhibition	1854:1876	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	1	67	theme	chemical	200:207	arg1	composition					209:219	the chemical composition	196:219	the chemical composition	196:219	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	0	68	theme	Mixture	137:143	arg1	Investigation					40:52	Investigation	40:52	Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture	40:143	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	0	68	theme	Mixture	137:143	arg1	Determination					0:12	Determination	0:12	Determination of Chemical Compounds	0:34	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	10	69	theme	265.57	1699:1704	arg1	μg/mL					1713:1717	265.57 ± 0.03 μg/mL	1699:1717	265.57 ± 0.03 μg/mL	1699:1717	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	10	69	theme	265.57	1699:1704	arg1	α-glucosidase					1684:1696	α-glucosidase	1684:1696	α-glucosidase (265.57 ± 0.03 μg/mL)	1684:1718	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	5	70	theme	EOs	884:886	arg1	activity					872:879	The antioxidant activity	856:879	The antioxidant activity of EOs	856:886	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	9	71	theme	±	1608:1608	arg1	µg/mL					1616:1620	533.89 ± 15.05 µg/mL	1601:1620	533.89 ± 15.05 µg/mL	1601:1620	The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL.
36144586	7	72	theme	composition	1216:1226	arg1	analysis					1186:1193	The analysis	1182:1193	The analysis of the phytochemical composition by GC-MS	1182:1235	The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), β-myrcene (8.01%), and chamazulene (6.54%).
36144586	5	73	theme	DPPH	942:945	arg1	ability					933:939	the free radical scavenging ability	905:939	the free radical scavenging ability (DPPH method)	905:953	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	5	73	theme	DPPH	942:945	arg1	method					947:952	DPPH method	942:952	DPPH method	942:952	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	9	74	theme	µg/mL	1616:1620	arg1	IC50					1593:1596	an IC50	1590:1596	an IC50 of 533.89 ± 15.05 µg/mL	1590:1620	The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL.
36144586	13	75	theme	chamomilla	2206:2215	arg1	target					2243:2248	a potential drug target	2226:2248	a potential drug target against diabetes, inflammation and microbial infections	2226:2304	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	13	75	theme	chamomilla	2206:2215	arg1	EOs					2196:2198	the EOs	2192:2198	the EOs of M. chamomilla	2192:2215	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	11	76	theme	high	1818:1821	arg1	effect					1841:1846	a high anti-inflammatory effect	1816:1846	a high anti-inflammatory effect	1816:1846	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	12	77	from	impact	1893:1898	arg1	species					1922:1928	the studied species	1910:1928	the studied species	1910:1928	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	78	theme	EOs	1903:1905	arg1	impact					1893:1898	The impact	1889:1898	The impact of EOs on the studied species	1889:1928	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	3	79	theme	disc	653:656	arg1	assay					668:672	disc diffusion assay	653:672	disc diffusion assay	653:672	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	5	80	theme	radical	914:920	arg1	scavenging					922:931	the free radical scavenging	905:931	the free radical scavenging ability (DPPH method)	905:953	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	0	81	theme	chamomilla	93:102	arg1	Oils					114:117	Matricaria chamomilla Essential Oils	82:117	Matricaria chamomilla Essential Oils	82:117	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	1	82	theme	exploratory	151:161	arg1	investigation					163:175	This exploratory investigation	146:175	This exploratory investigation	146:175	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	2	83	theme	gas	497:499	arg1	spectrophotometry					521:537	gas chromatography-mass spectrophotometry	497:537	gas chromatography-mass spectrophotometry (GC-MS)	497:545	EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography-mass spectrophotometry (GC-MS).
36144586	2	83	theme	gas	497:499	arg1	GC-MS					540:544	GC-MS	540:544	GC-MS	540:544	EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography-mass spectrophotometry (GC-MS).
36144586	0	84	theme	Oils	114:117	arg1	Properties					68:77	Biological Properties	57:77	Biological Properties of Matricaria chamomilla Essential Oils	57:117	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	0	84	theme	Oils	114:117	arg1	Mixture					137:143	Their Mixture	131:143	Their Mixture	131:143	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	0	84	theme	Oils	114:117	arg1	Honey					120:124	Honey	120:124	Honey	120:124	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	1	85	theme	L.	371:372	arg1	oils					384:387	Matricaria chamomilla L. essential oils	349:387	Matricaria chamomilla L. essential oils (EOs)	349:393	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	1	85	theme	L.	371:372	arg1	EOs					390:392	EOs	390:392	EOs	390:392	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	11	86	theme	edema	1861:1865	arg1	inhibition					1867:1876	63.75% edema inhibition	1854:1876	63.75% edema inhibition	1854:1876	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	0	87	theme	Honey	120:124	arg1	Investigation					40:52	Investigation	40:52	Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture	40:143	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	0	87	theme	Honey	120:124	arg1	Determination					0:12	Determination	0:12	Determination of Chemical Compounds	0:34	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	1	88	theme	oils	384:387	arg1	properties					250:259	some biological properties	234:259	some biological properties	234:259	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	1	88	theme	oils	384:387	arg1	activities					334:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	6	89	theme	paw	1105:1107	arg1	method					1115:1120	the carrageenan-induced paw edema method	1081:1120	the carrageenan-induced paw edema method	1081:1120	The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test.
36144586	12	90	theme	0.18	2057:2060	arg1	mm					2062:2063	18.13 ± 0.18 mm	2049:2063	18.13 ± 0.18 mm	2049:2063	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	90	theme	0.18	2057:2060	arg1	niger					2042:2046	Aspergillus niger	2030:2046	Aspergillus niger (18.13 ± 0.18 mm)	2030:2064	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	91	theme	Aspergillus	2030:2040	arg1	mm					2062:2063	18.13 ± 0.18 mm	2049:2063	18.13 ± 0.18 mm	2049:2063	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	91	theme	Aspergillus	2030:2040	arg1	niger					2042:2046	Aspergillus niger	2030:2046	Aspergillus niger (18.13 ± 0.18 mm)	2030:2064	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	5	92	theme	ferrous	956:962	arg1	chelating					968:976	ferrous ion chelating	956:976	ferrous ion chelating (FIC) ability	956:990	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	5	92	theme	ferrous	956:962	arg1	FIC					979:981	FIC	979:981	FIC	979:981	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	13	93	theme	potential	2228:2236	arg1	target					2243:2248	a potential drug target	2226:2248	a potential drug target against diabetes, inflammation and microbial infections	2226:2304	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	13	93	theme	potential	2228:2236	arg1	EOs					2196:2198	the EOs	2192:2198	the EOs of M. chamomilla	2192:2215	The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.
36144586	0	94	theme	Compounds	26:34	arg1	Investigation					40:52	Investigation	40:52	Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture	40:143	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	0	94	theme	Compounds	26:34	arg1	Determination					0:12	Determination	0:12	Determination of Chemical Compounds	0:34	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	5	95	theme	chelating	968:976	arg1	ability					984:990	ferrous ion chelating (FIC) ability	956:990	ferrous ion chelating (FIC) ability	956:990	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	8	96	theme	±	1484:1484	arg1	µg/mL					1492:1496	533.89 ± 15.05 µg/mL	1477:1496	533.89 ± 15.05 µg/mL	1477:1496	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	10	97	theme	121.44	1735:1740	arg1	μg/mL					1749:1753	121.44 ± 0.05 μg/mL	1735:1753	121.44 ± 0.05 μg/mL	1735:1753	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	10	97	theme	121.44	1735:1740	arg1	α-amylase					1724:1732	α-amylase	1724:1732	α-amylase (121.44 ± 0.05 μg/mL)	1724:1754	As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL).
36144586	4	98	theme	antidiabetic	777:788	arg1	activity					790:797	The antidiabetic activity	773:797	The antidiabetic activity	773:797	The antidiabetic activity was performed in vitro using the enzyme inhibition test.
36144586	3	99	dep	assay	668:672	arg1	methods					764:770	methods	764:770	methods	764:770	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	2	100	theme	M.	403:404	arg1	chamomilla					406:415	M. chamomilla	403:415	M. chamomilla	403:415	EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography-mass spectrophotometry (GC-MS).
36144586	0	101	theme	Biological	57:66	arg1	Properties					68:77	Biological Properties	57:77	Biological Properties of Matricaria chamomilla Essential Oils	57:117	Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture.
36144586	1	102	theme	antimicrobial	320:332	arg1	activities					334:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	11	103	dep	EOs	1761:1763	arg1	The					1757:1759	The	1757:1759	The	1757:1759	The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h.
36144586	7	104	theme	major	1276:1280	arg1	camphor					1251:1257	camphor	1251:1257	camphor (16.42%)	1251:1266	The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), β-myrcene (8.01%), and chamazulene (6.54%).
36144586	7	104	theme	major	1276:1280	arg1	compound					1282:1289	the major compound	1272:1289	the major compound of EOs	1272:1296	The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), β-myrcene (8.01%), and chamazulene (6.54%).
36144586	8	105	theme	1945.38	1501:1507	arg1	µg/mL					1517:1521	1945.38 ± 12.71 µg/mL	1501:1521	1945.38 ± 12.71 µg/mL	1501:1521	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	12	106	theme	ATCC	1987:1990	arg1	mm					2012:2013	22.97 ± 0.16 mm	1999:2013	22.97 ± 0.16 mm	1999:2013	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	106	theme	ATCC	1987:1990	arg1	29213					1992:1996	Staphylococcus aureus ATCC 29213	1965:1996	Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)	1965:2014	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	8	107	theme	12.71	1511:1515	arg1	µg/mL					1517:1521	1945.38 ± 12.71 µg/mL	1501:1521	1945.38 ± 12.71 µg/mL	1501:1521	MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL).
36144586	12	108	theme	Staphylococcus	1965:1978	arg1	mm					2012:2013	22.97 ± 0.16 mm	1999:2013	22.97 ± 0.16 mm	1999:2013	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	12	108	theme	Staphylococcus	1965:1978	arg1	29213					1992:1996	Staphylococcus aureus ATCC 29213	1965:1996	Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)	1965:2014	The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains.
36144586	1	109	theme	biological	239:248	arg1	properties					250:259	some biological properties	234:259	some biological properties	234:259	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	1	109	theme	biological	239:248	arg1	activities					334:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities	270:343	This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs).
36144586	5	110	theme	bleaching	1008:1016	arg1	assay					1018:1022	β-carotene bleaching assay	997:1022	β-carotene bleaching assay	997:1022	The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay.
36144586	3	111	theme	bactericidal	731:742	arg1	MBC					759:761	MBC	759:761	MBC	759:761	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	3	111	theme	bactericidal	731:742	arg1	concentration					744:756	minimum bactericidal concentration	723:756	minimum bactericidal concentration (MBC)	723:762	The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods.
36144586	4	112	theme	enzyme	832:837	arg1	test					850:853	the enzyme inhibition test	828:853	the enzyme inhibition test	828:853	The antidiabetic activity was performed in vitro using the enzyme inhibition test.
37219870	0	0	theme	Hydrogen	0:7	arg1	fractionation					17:29	Hydrogen isotope fractionation	0:29	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues	0:74	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	2	1	theme	Northern	566:573	arg1	tree					586:589	73 Northern Hemisphere tree and shrub species	563:607	tree	586:589	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	1	2	theme	methodological	182:195	arg1	advancements					197:208	Recent methodological advancements	175:208	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates	175:306	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	3	3	theme	distinct	687:694	arg1	patterns					709:716	distinct phylogenetic patterns	687:716	distinct phylogenetic patterns	687:716	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	6	4	theme	δ2	1239:1240	arg1	Hne					1242:1244	δ2 Hne	1239:1244	δ2 Hne of leaf sugars	1239:1259	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	6	5	theme	signals	1228:1234	arg1	strength					1199:1206	the strength	1195:1206	the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose	1195:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	1	6	theme	processes	392:400	arg1	drivers					344:350	the drivers	340:350	the drivers of hydrogen isotope (2 H) fractionation processes in plants	340:410	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	6	7	from	signals	1228:1234	arg1	cellulose					1276:1284	twig xylem cellulose	1265:1284	twig xylem cellulose	1265:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	6	7	from	signals	1228:1234	arg1	Hne					1242:1244	δ2 Hne	1239:1244	δ2 Hne of leaf sugars	1239:1259	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	3	8	from	fractionation	641:653	arg1	carbohydrates					664:676	plant carbohydrates	658:676	plant carbohydrates	658:676	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	2	9	theme	leaf	528:531	arg1	sugars					533:538	leaf sugars	528:538	leaf sugars	528:538	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	0	10	with	experiment	132:141	arg1	tree					151:154	73 tree	148:154	73 tree	148:154	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	0	10	with	experiment	132:141	arg1	species					166:172	shrub species	160:172	shrub species	160:172	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	3	11	theme	δ2	756:757	arg1	Hne					759:761	the δ2 Hne	752:761	the δ2 Hne of leaf sugars	752:776	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	2	12	theme	twig	479:482	arg1	cellulose					490:498	twig xylem cellulose	479:498	twig xylem cellulose	479:498	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	0	13	theme	xylem	62:66	arg1	tissues					68:74	xylem tissues	62:74	xylem tissues	62:74	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	7	14	theme	important	1500:1508	arg1	consequences					1510:1521	important consequences	1500:1521	important consequences for dendrochronological and ecophysiological studies	1500:1574	Our results will help improve 2 H fractionation models for plant carbohydrates and have important consequences for dendrochronological and ecophysiological studies.
37219870	0	15	from	fractionation	17:29	arg1	carbohydrates					34:46	carbohydrates	34:46	carbohydrates of leaves and xylem tissues	34:74	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	3	16	theme	sugars	771:776	arg1	Hne					759:761	the δ2 Hne	752:761	the δ2 Hne of leaf sugars	752:776	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	6	17	theme	twig	1265:1268	arg1	cellulose					1276:1284	twig xylem cellulose	1265:1284	twig xylem cellulose	1265:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	3	18	theme	xylem	799:803	arg1	cellulose					805:813	twig xylem cellulose	794:813	twig xylem cellulose	794:813	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	2	19	theme	xylem	504:508	arg1	water					510:514	xylem water	504:514	xylem water	504:514	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	0	20	theme	phylogenetic	93:104	arg1	patterns					106:113	distinct phylogenetic patterns	84:113	distinct phylogenetic patterns	84:113	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	1	21	theme	isotope	364:370	arg1	processes					392:400	hydrogen isotope (2 H) fractionation processes	355:400	hydrogen isotope (2 H) fractionation processes	355:400	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	7	22	theme	plant	1471:1475	arg1	carbohydrates					1477:1489	plant carbohydrates	1471:1489	plant carbohydrates	1471:1489	Our results will help improve 2 H fractionation models for plant carbohydrates and have important consequences for dendrochronological and ecophysiological studies.
37219870	2	23	theme	cellulose	490:498	arg1	sugars					533:538	leaf sugars	528:538	leaf sugars	528:538	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	2	23	theme	cellulose	490:498	arg1	Hne					472:474	the δ2 Hne	465:474	the δ2 Hne of twig xylem cellulose and xylem water	465:514	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	2	23	theme	cellulose	490:498	arg1	water					549:553	leaf water	544:553	leaf water	544:553	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	4	24	contain	had	826:828	arg2	influence					844:852	no detectable influence	830:852	no detectable influence	830:852	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	4	24	contain	had	826:828	arg1	Phylogeny					816:824	Phylogeny	816:824	Phylogeny	816:824	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	2	25	theme	common	622:627	arg1	garden					629:634	a common garden	620:634	a common garden	620:634	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	6	26	theme	autotrophic	1335:1345	arg1	processes					1347:1355	autotrophic processes	1335:1355	autotrophic processes	1335:1355	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	5	27	theme	Hne	1090:1092	arg1	variations					1094:1103	substantial δ2 Hne variations	1075:1103	substantial δ2 Hne variations	1075:1103	Angiosperms were more 2 H-enriched than gymnosperms, but substantial δ2 Hne variations also occurred at the order, family, and species levels within both clades.
37219870	0	28	dep	follows	76:82	arg1	experiment					132:141	a common garden experiment	116:141	a common garden experiment with 73 tree and shrub species	116:172	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	1	29	theme	carbohydrates	294:306	arg1	composition					263:273	the nonexchangeable hydrogen isotopic composition	225:273	the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates	225:306	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	1	29	theme	carbohydrates	294:306	arg1	Hne					279:281	δ2 Hne	276:281	δ2 Hne	276:281	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	7	30	theme	fractionation	1446:1458	arg1	models					1460:1465	2 H fractionation models	1442:1465	2 H fractionation models for plant carbohydrates	1442:1489	Our results will help improve 2 H fractionation models for plant carbohydrates and have important consequences for dendrochronological and ecophysiological studies.
37219870	6	31	theme	original	1303:1310	arg1	signal					1325:1330	the original phylogenetic signal	1299:1330	the original phylogenetic signal of autotrophic processes	1299:1355	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	7	32	theme	2	1442:1442	arg1	H					1444:1444	H	1444:1444	H	1444:1444	Our results will help improve 2 H fractionation models for plant carbohydrates and have important consequences for dendrochronological and ecophysiological studies.
37219870	4	33	theme	isotopic	922:929	arg1	biochemistry					904:915	biochemistry	904:915	biochemistry	904:915	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	4	33	theme	isotopic	922:929	arg1	differences					931:941	not isotopic differences	918:941	not isotopic differences in plant water	918:956	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	1	34	from	drivers	344:350	arg1	plants					405:410	plants	405:410	plants	405:410	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	5	35	theme	substantial	1075:1085	arg1	variations					1094:1103	substantial δ2 Hne variations	1075:1103	substantial δ2 Hne variations	1075:1103	Angiosperms were more 2 H-enriched than gymnosperms, but substantial δ2 Hne variations also occurred at the order, family, and species levels within both clades.
37219870	6	36	from	Differences	1180:1190	arg1	strength					1199:1206	the strength	1195:1206	the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose	1195:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	6	37	theme	species-specific	1383:1398	arg1	metabolism					1400:1409	subsequent species-specific metabolism	1372:1409	subsequent species-specific metabolism	1372:1409	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	1	38	theme	isotopic	254:261	arg1	composition					263:273	the nonexchangeable hydrogen isotopic composition	225:273	the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates	225:306	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	1	38	theme	isotopic	254:261	arg1	Hne					279:281	δ2 Hne	276:281	δ2 Hne	276:281	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	2	39	theme	phylogeny	452:460	arg1	influence					439:447	the influence	435:447	the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water	435:553	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	6	40	theme	xylem	1270:1274	arg1	cellulose					1276:1284	twig xylem cellulose	1265:1284	twig xylem cellulose	1265:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	4	41	theme	observed	970:977	arg1	pattern					992:998	the observed phylogenetic pattern	966:998	the observed phylogenetic pattern in carbohydrates	966:1015	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	3	42	theme	2	637:637	arg1	H					639:639	H	639:639	H	639:639	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	5	43	theme	order	1126:1130	arg1	levels					1153:1158	the order, family, and species levels	1122:1158	the order, family, and species levels within both clades	1122:1177	Angiosperms were more 2 H-enriched than gymnosperms, but substantial δ2 Hne variations also occurred at the order, family, and species levels within both clades.
37219870	2	44	theme	leaf	544:547	arg1	water					549:553	leaf water	544:553	leaf water	544:553	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	0	45	theme	leaves	51:56	arg1	carbohydrates					34:46	carbohydrates	34:46	carbohydrates of leaves and xylem tissues	34:74	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	2	46	theme	Hemisphere	575:584	arg1	tree					586:589	73 Northern Hemisphere tree and shrub species	563:607	tree	586:589	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	3	47	theme	plant	658:662	arg1	carbohydrates					664:676	plant carbohydrates	658:676	plant carbohydrates	658:676	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	1	48	theme	Recent	175:180	arg1	advancements					197:208	Recent methodological advancements	175:208	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates	175:306	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	1	49	theme	nonexchangeable	229:243	arg1	composition					263:273	the nonexchangeable hydrogen isotopic composition	225:273	the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates	225:306	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	1	49	theme	nonexchangeable	229:243	arg1	Hne					279:281	δ2 Hne	276:281	δ2 Hne	276:281	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	0	50	theme	garden	125:130	arg1	experiment					132:141	a common garden experiment	116:141	a common garden experiment with 73 tree and shrub species	116:172	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	3	51	theme	phylogenetic	696:707	arg1	patterns					709:716	distinct phylogenetic patterns	687:716	distinct phylogenetic patterns	687:716	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	4	52	dep	twig	871:874	arg1	water					884:888	water	884:888	water	884:888	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	1	53	theme	fractionation	378:390	arg1	processes					392:400	hydrogen isotope (2 H) fractionation processes	355:400	hydrogen isotope (2 H) fractionation processes	355:400	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	7	54	theme	ecophysiological	1551:1566	arg1	studies					1568:1574	dendrochronological and ecophysiological studies	1527:1574	dendrochronological and ecophysiological studies	1527:1574	Our results will help improve 2 H fractionation models for plant carbohydrates and have important consequences for dendrochronological and ecophysiological studies.
37219870	4	55	theme	δ2	861:862	arg1	Hne					864:866	the δ2 Hne	857:866	the δ2 Hne of twig or leaf water	857:888	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	4	56	theme	twig	871:874	arg1	Hne					864:866	the δ2 Hne	857:866	the δ2 Hne of twig or leaf water	857:888	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	6	57	theme	phylogenetic	1215:1226	arg1	signals					1228:1234	the phylogenetic signals	1211:1234	the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose	1211:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	6	58	from	cellulose	1276:1284	arg1	strength					1199:1206	the strength	1195:1206	the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose	1195:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	7	59	theme	dendrochronological	1527:1545	arg1	studies					1568:1574	dendrochronological and ecophysiological studies	1527:1574	dendrochronological and ecophysiological studies	1527:1574	Our results will help improve 2 H fractionation models for plant carbohydrates and have important consequences for dendrochronological and ecophysiological studies.
37219870	0	60	theme	shrub	160:164	arg1	species					166:172	shrub species	160:172	shrub species	160:172	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	4	61	from	pattern	992:998	arg1	carbohydrates					1003:1015	carbohydrates	1003:1015	carbohydrates	1003:1015	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	3	62	theme	twig	794:797	arg1	cellulose					805:813	twig xylem cellulose	794:813	twig xylem cellulose	794:813	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	4	63	theme	detectable	833:842	arg1	influence					844:852	no detectable influence	830:852	no detectable influence	830:852	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	0	64	theme	tissues	68:74	arg1	carbohydrates					34:46	carbohydrates	34:46	carbohydrates of leaves and xylem tissues	34:74	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	5	65	theme	family	1133:1138	arg1	levels					1153:1158	the order, family, and species levels	1122:1158	the order, family, and species levels within both clades	1122:1177	Angiosperms were more 2 H-enriched than gymnosperms, but substantial δ2 Hne variations also occurred at the order, family, and species levels within both clades.
37219870	6	66	theme	sugars	1254:1259	arg1	cellulose					1276:1284	twig xylem cellulose	1265:1284	twig xylem cellulose	1265:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	6	66	theme	sugars	1254:1259	arg1	Hne					1242:1244	δ2 Hne	1239:1244	δ2 Hne of leaf sugars	1239:1259	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	0	67	theme	distinct	84:91	arg1	patterns					106:113	distinct phylogenetic patterns	84:113	distinct phylogenetic patterns	84:113	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	2	68	theme	δ2	469:470	arg1	Hne					472:474	the δ2 Hne	465:474	the δ2 Hne of twig xylem cellulose and xylem water	465:514	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	2	69	from	influence	439:447	arg1	sugars					533:538	leaf sugars	528:538	leaf sugars	528:538	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	2	69	from	influence	439:447	arg1	Hne					472:474	the δ2 Hne	465:474	the δ2 Hne of twig xylem cellulose and xylem water	465:514	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	2	69	from	influence	439:447	arg1	water					549:553	leaf water	544:553	leaf water	544:553	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	6	70	from	Hne	1242:1244	arg1	strength					1199:1206	the strength	1195:1206	the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose	1195:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	1	71	theme	hydrogen	355:362	arg1	isotope					364:370	hydrogen isotope	355:370	hydrogen isotope (2 H) fractionation processes	355:400	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	1	71	theme	hydrogen	355:362	arg1	H					375:375	2 H	373:375	2 H	373:375	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	2	72	theme	shrub	595:599	arg1	species					601:607	73 Northern Hemisphere tree and shrub species	563:607	species	601:607	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	3	73	theme	leaf	766:769	arg1	sugars					771:776	leaf sugars	766:776	leaf sugars	766:776	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	6	74	theme	leaf	1249:1252	arg1	sugars					1254:1259	leaf sugars	1249:1259	leaf sugars	1249:1259	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	2	75	theme	xylem	484:488	arg1	cellulose					490:498	twig xylem cellulose	479:498	twig xylem cellulose	479:498	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	4	76	theme	leaf	879:882	arg1	Hne					864:866	the δ2 Hne	857:866	the δ2 Hne of twig or leaf water	857:888	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	0	77	theme	isotope	9:15	arg1	fractionation					17:29	Hydrogen isotope fractionation	0:29	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues	0:74	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	1	78	theme	plant	288:292	arg1	carbohydrates					294:306	plant carbohydrates	288:306	plant carbohydrates	288:306	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	4	79	theme	plant	946:950	arg1	water					952:956	plant water	946:956	plant water	946:956	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	6	80	theme	phylogenetic	1312:1323	arg1	signal					1325:1330	the original phylogenetic signal	1299:1330	the original phylogenetic signal of autotrophic processes	1299:1355	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	4	81	from	differences	931:941	arg1	water					952:956	plant water	946:956	plant water	946:956	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	6	82	from	strength	1199:1206	arg1	cellulose					1276:1284	twig xylem cellulose	1265:1284	twig xylem cellulose	1265:1284	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	6	82	from	strength	1199:1206	arg1	Hne					1242:1244	δ2 Hne	1239:1244	δ2 Hne of leaf sugars	1239:1259	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	7	83	theme	H	1444:1444	arg1	models					1460:1465	2 H fractionation models	1442:1465	2 H fractionation models for plant carbohydrates	1442:1489	Our results will help improve 2 H fractionation models for plant carbohydrates and have important consequences for dendrochronological and ecophysiological studies.
37219870	2	84	theme	water	510:514	arg1	sugars					533:538	leaf sugars	528:538	leaf sugars	528:538	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	2	84	theme	water	510:514	arg1	Hne					472:474	the δ2 Hne	465:474	the δ2 Hne of twig xylem cellulose and xylem water	465:514	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	2	84	theme	water	510:514	arg1	water					549:553	leaf water	544:553	leaf water	544:553	Here, we investigated the influence of phylogeny on the δ2 Hne of twig xylem cellulose and xylem water, as well as leaf sugars and leaf water, across 73 Northern Hemisphere tree and shrub species growing in a common garden.
37219870	5	85	theme	δ2	1087:1088	arg1	variations					1094:1103	substantial δ2 Hne variations	1075:1103	substantial δ2 Hne variations	1075:1103	Angiosperms were more 2 H-enriched than gymnosperms, but substantial δ2 Hne variations also occurred at the order, family, and species levels within both clades.
37219870	4	86	theme	phylogenetic	979:990	arg1	pattern					992:998	the observed phylogenetic pattern	966:998	the observed phylogenetic pattern in carbohydrates	966:1015	Phylogeny had no detectable influence on the δ2 Hne of twig or leaf water, showing that biochemistry, not isotopic differences in plant water, caused the observed phylogenetic pattern in carbohydrates.
37219870	1	87	theme	hydrogen	245:252	arg1	composition					263:273	the nonexchangeable hydrogen isotopic composition	225:273	the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates	225:306	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	1	87	theme	hydrogen	245:252	arg1	Hne					279:281	δ2 Hne	276:281	δ2 Hne	276:281	Recent methodological advancements in determining the nonexchangeable hydrogen isotopic composition (δ2 Hne ) of plant carbohydrates make it possible to disentangle the drivers of hydrogen isotope (2 H) fractionation processes in plants.
37219870	0	88	theme	common	118:123	arg1	experiment					132:141	a common garden experiment	116:141	a common garden experiment with 73 tree and shrub species	116:172	Hydrogen isotope fractionation in carbohydrates of leaves and xylem tissues follows distinct phylogenetic patterns: a common garden experiment with 73 tree and shrub species.
37219870	3	89	theme	H	639:639	arg1	fractionation					641:653	2 H fractionation	637:653	2 H fractionation in plant carbohydrates	637:676	2 H fractionation in plant carbohydrates followed distinct phylogenetic patterns, with phylogeny reflected more in the δ2 Hne of leaf sugars than in that of twig xylem cellulose.
37219870	6	90	theme	processes	1347:1355	arg1	signal					1325:1330	the original phylogenetic signal	1299:1330	the original phylogenetic signal of autotrophic processes	1299:1355	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	6	91	theme	subsequent	1372:1381	arg1	metabolism					1400:1409	subsequent species-specific metabolism	1372:1409	subsequent species-specific metabolism	1372:1409	Differences in the strength of the phylogenetic signals in δ2 Hne of leaf sugars and twig xylem cellulose suggest that the original phylogenetic signal of autotrophic processes was altered by subsequent species-specific metabolism.
37219870	5	92	theme	species	1145:1151	arg1	levels					1153:1158	the order, family, and species levels	1122:1158	the order, family, and species levels within both clades	1122:1177	Angiosperms were more 2 H-enriched than gymnosperms, but substantial δ2 Hne variations also occurred at the order, family, and species levels within both clades.
36253733	0	0	from	characteristics	19:33	arg1	chinense					109:116	Liriodendron chinense	96:116	Liriodendron chinense	96:116	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	8	1	from	times	1479:1483	arg1	different					1456:1464	different	1456:1464	different	1456:1464	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	8	1	from	times	1479:1483	arg1	patterns					1432:1439	the expression patterns	1417:1439	the expression patterns of LcSnRKs	1417:1450	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	4	2	dep	subfamilies	729:739	arg1	subfamilies					729:739	three subfamilies: SnRK1, SnRK2, and SnRK3	723:764	three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types	723:812	It could be divided into three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types.
36253733	4	2	dep	subfamilies	729:739	arg1	SnRK2					749:753	SnRK2	749:753	SnRK2	749:753	It could be divided into three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types.
36253733	4	2	dep	subfamilies	729:739	arg1	SnRK3					760:764	SnRK3	760:764	SnRK3	760:764	It could be divided into three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types.
36253733	4	2	dep	subfamilies	729:739	arg1	SnRK1					742:746	SnRK1	742:746	SnRK1	742:746	It could be divided into three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types.
36253733	0	3	theme	profiles	84:91	arg1	characteristics					19:33	characteristics	19:33	characteristics	19:33	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	0	3	theme	profiles	84:91	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	7	4	theme	different	1370:1378	arg1	stresses					1394:1401	different environmental stresses	1370:1401	different environmental stresses	1370:1401	Cis-regulation element analysis showed that LcSnRKs were LTR and TC-rich, which could respond to different environmental stresses.
36253733	12	5	theme	LcSnRK	1883:1888	arg1	gene					1890:1893	the LcSnRK gene	1879:1893	the LcSnRK gene	1879:1893	CONCLUSION The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.
36253733	5	6	theme	subfamilies	839:849	arg1	LcSnRK					819:824	The LcSnRK	815:824	The LcSnRK of the three subfamilies	815:849	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	1	7	theme	-related	165:172	arg1	SnRKs					191:195	SnRKs	191:195	SnRKs	191:195	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	1	7	theme	-related	165:172	arg1	kinases					182:188	The sucrose non-fermenting 1 (SNF1)-related protein kinases	130:188	The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs)	130:196	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	0	8	from	Identification	0:13	arg1	chinense					109:116	Liriodendron chinense	96:116	Liriodendron chinense	96:116	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	3	9	theme	present	537:543	arg1	study					545:549	the present study	533:549	the present study	533:549	RESULTS In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds.
36253733	1	10	theme	stress	251:256	arg1	response					258:265	stress response	251:265	stress response	251:265	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	0	11	theme	Liriodendron	96:107	arg1	chinense					109:116	Liriodendron chinense	96:116	Liriodendron chinense	96:116	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	11	12	theme	expression	1680:1689	arg1	trend					1691:1695	The expression trend	1676:1695	The expression trend of LcSnRK3s under low-temperature stress	1676:1736	The expression trend of LcSnRK3s under low-temperature stress was mainly up-or down-regulated.
36253733	5	13	theme	motif	914:918	arg1	composition					920:930	motif composition	914:930	motif composition	914:930	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	12	14	dep	CONCLUSION	1771:1780	arg1	provide					1813:1819	provide	1813:1819	will provide valuable information for the functional identification of the LcSnRK gene in the future	1808:1907	CONCLUSION The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.
36253733	6	15	theme	gene	1049:1052	arg1	pairs					1054:1058	13 collinear gene pairs	1036:1058	13 collinear gene pairs	1036:1058	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	1	16	theme	protein	174:180	arg1	SnRKs					191:195	SnRKs	191:195	SnRKs	191:195	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	1	16	theme	protein	174:180	arg1	kinases					182:188	The sucrose non-fermenting 1 (SNF1)-related protein kinases	130:188	The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs)	130:196	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	2	17	theme	stress	503:508	arg1	conditions					510:519	stress conditions	503:519	stress conditions	503:519	Conducting identification and stress response studies on SnRKs in plants contributes to the development of strategies for tree species that are more tolerant to stress conditions.
36253733	2	18	theme	strategies	449:458	arg1	development					434:444	the development	430:444	the development of strategies for tree species that are more tolerant to stress conditions	430:519	Conducting identification and stress response studies on SnRKs in plants contributes to the development of strategies for tree species that are more tolerant to stress conditions.
36253733	8	19	theme	LcSnRKs	1444:1450	arg1	different					1456:1464	different	1456:1464	different	1456:1464	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	8	19	theme	LcSnRKs	1444:1450	arg1	patterns					1432:1439	the expression patterns	1417:1439	the expression patterns of LcSnRKs	1417:1450	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	6	20	theme	L.	1159:1160	arg1	chinense					1162:1169	L. chinense	1159:1169	L. chinense	1159:1169	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	12	21	theme	study	1802:1806	arg1	results					1786:1792	The results	1782:1792	The results of this study	1782:1806	CONCLUSION The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.
36253733	1	22	theme	stress	326:331	arg1	signals					333:339	stress signals	326:339	stress signals	326:339	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	8	23	theme	expression	1421:1430	arg1	different					1456:1464	different	1456:1464	different	1456:1464	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	8	23	theme	expression	1421:1430	arg1	patterns					1432:1439	the expression patterns	1417:1439	the expression patterns of LcSnRKs	1417:1450	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	11	24	theme	low-temperature	1715:1729	arg1	stress					1731:1736	low-temperature stress	1715:1736	low-temperature stress	1715:1736	The expression trend of LcSnRK3s under low-temperature stress was mainly up-or down-regulated.
36253733	4	25	theme	domain	801:806	arg1	types					808:812	domain types	801:812	domain types	801:812	It could be divided into three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types.
36253733	12	26	from	identification	1861:1874	arg1	future					1902:1907	future	1902:1907	future	1902:1907	CONCLUSION The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.
36253733	5	27	theme	kinase	978:983	arg1	domain					985:990	the kinase domain	974:990	the kinase domain	974:990	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	2	28	theme	response	379:386	arg1	studies					388:394	stress response studies	372:394	stress response studies	372:394	Conducting identification and stress response studies on SnRKs in plants contributes to the development of strategies for tree species that are more tolerant to stress conditions.
36253733	12	29	theme	gene	1890:1893	arg1	identification					1861:1874	the functional identification	1846:1874	the functional identification of the LcSnRK gene in the future	1846:1907	CONCLUSION The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.
36253733	7	30	theme	Cis-regulation	1273:1286	arg1	analysis					1296:1303	Cis-regulation element analysis	1273:1303	Cis-regulation element analysis	1273:1303	Cis-regulation element analysis showed that LcSnRKs were LTR and TC-rich, which could respond to different environmental stresses.
36253733	5	31	theme	gene	895:898	arg1	structure					900:908	gene structure	895:908	gene structure	895:908	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	9	32	theme	LcSnRK1s	1515:1522	arg1	expression					1524:1533	LcSnRK1s expression	1515:1533	LcSnRK1s expression	1515:1533	LcSnRK1s expression tended to be down-regulated under low-temperature stress.
36253733	5	33	theme	significant	1000:1010	arg1	differences					1012:1022	significant differences	1000:1022	significant differences	1000:1022	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	3	34	dep	RESULTS	522:528	arg1	identified					579:588	identified	579:588	were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds	574:695	RESULTS In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds.
36253733	6	35	theme	collinear	1039:1047	arg1	pairs					1054:1058	13 collinear gene pairs	1036:1058	13 collinear gene pairs	1036:1058	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	0	36	theme	genes	43:47	arg1	characteristics					19:33	characteristics	19:33	characteristics	19:33	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	0	36	theme	genes	43:47	arg1	Identification					0:13	Identification	0:13	Identification	0:13	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	8	37	theme	different	1469:1477	arg1	times					1479:1483	different times	1469:1483	different times	1469:1483	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	12	38	theme	functional	1850:1859	arg1	identification					1861:1874	the functional identification	1846:1874	the functional identification of the LcSnRK gene in the future	1846:1907	CONCLUSION The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.
36253733	7	39	theme	element	1288:1294	arg1	analysis					1296:1303	Cis-regulation element analysis	1273:1303	Cis-regulation element analysis	1273:1303	Cis-regulation element analysis showed that LcSnRKs were LTR and TC-rich, which could respond to different environmental stresses.
36253733	6	40	dep	chinense	1080:1087	arg1	thaliana					1118:1125	A. thaliana	1115:1125	A. thaliana	1115:1125	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	10	41	theme	LcSnRK2s	1611:1618	arg1	expression					1597:1606	The expression	1593:1606	The expression of LcSnRK2s	1593:1618	The expression of LcSnRK2s tended to be up-regulated under low-temperature stress.
36253733	0	42	theme	SnRK	38:41	arg1	genes					43:47	SnRK genes	38:47	SnRK genes	38:47	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	8	43	theme	low-temperature	1491:1505	arg1	stress					1507:1512	low-temperature stress	1491:1512	low-temperature stress	1491:1512	Furthermore, the expression patterns of LcSnRKs are different at different times under low-temperature stress.
36253733	3	44	theme	L.	616:617	arg1	genome					629:634	Liriodendron chinense (L. chinense) genome	593:634	Liriodendron chinense (L. chinense) genome	593:634	RESULTS In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds.
36253733	1	45	theme	vivid	205:209	arg1	role					211:214	a vivid role	203:214	a vivid role	203:214	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	2	46	theme	tree	464:467	arg1	species					469:475	tree species	464:475	tree species that are more tolerant to stress conditions	464:519	Conducting identification and stress response studies on SnRKs in plants contributes to the development of strategies for tree species that are more tolerant to stress conditions.
36253733	6	47	located	detected	1147:1154	arg1	chinense					1162:1169	L. chinense	1159:1169	L. chinense	1159:1169	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	6	47	located	detected	1147:1154	arg2	pairs					1136:1140	18 pairs	1133:1140	18 pairs	1133:1140	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	6	47	located	detected	1147:1154	arg1	rice					1175:1178	rice	1175:1178	rice	1175:1178	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	0	48	theme	stress-induced	58:71	arg1	profiles					84:91	cold stress-induced expression profiles	53:91	cold stress-induced expression profiles	53:91	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	4	49	theme	phylogenetic	775:786	arg1	analysis					788:795	phylogenetic analysis	775:795	phylogenetic analysis	775:795	It could be divided into three subfamilies: SnRK1, SnRK2, and SnRK3 based on phylogenetic analysis and domain types.
36253733	12	50	theme	valuable	1821:1828	arg1	information					1830:1840	valuable information	1821:1840	valuable information for the functional identification of the LcSnRK gene in the future	1821:1907	CONCLUSION The results of this study will provide valuable information for the functional identification of the LcSnRK gene in the future.
36253733	10	51	theme	low-temperature	1652:1666	arg1	stress					1668:1673	low-temperature stress	1652:1673	low-temperature stress	1652:1673	The expression of LcSnRK2s tended to be up-regulated under low-temperature stress.
36253733	7	52	theme	environmental	1380:1392	arg1	stresses					1394:1401	different environmental stresses	1370:1401	different environmental stresses	1370:1401	Cis-regulation element analysis showed that LcSnRKs were LTR and TC-rich, which could respond to different environmental stresses.
36253733	6	53	theme	family	1208:1213	arg1	genes					1215:1219	the LcSnRK family genes	1197:1219	the LcSnRK family genes	1197:1219	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	6	53	theme	family	1208:1213	arg1	related					1256:1262	related	1256:1262	related	1256:1262	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	3	54	theme	chinense	619:626	arg1	genome					629:634	Liriodendron chinense (L. chinense) genome	593:634	Liriodendron chinense (L. chinense) genome	593:634	RESULTS In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds.
36253733	3	55	theme	LcSnRKs	566:572	arg1	total					554:558	a total	552:558	a total of 30 LcSnRKs	552:572	RESULTS In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds.
36253733	2	56	from	SnRKs	399:403	arg1	plants					408:413	plants	408:413	plants	408:413	Conducting identification and stress response studies on SnRKs in plants contributes to the development of strategies for tree species that are more tolerant to stress conditions.
36253733	5	57	theme	same	862:865	arg1	structure					882:890	the same Ser/Thr kinase structure	858:890	the same Ser/Thr kinase structure in gene structure and motif composition	858:930	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	9	58	theme	low-temperature	1569:1583	arg1	stress					1585:1590	low-temperature stress	1569:1590	low-temperature stress	1569:1590	LcSnRK1s expression tended to be down-regulated under low-temperature stress.
36253733	6	59	theme	LcSnRK	1201:1206	arg1	genes					1215:1219	the LcSnRK family genes	1197:1219	the LcSnRK family genes	1197:1219	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	6	59	theme	LcSnRK	1201:1206	arg1	related					1256:1262	related	1256:1262	related	1256:1262	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	6	60	located	detected	1065:1072	arg1	chinense					1080:1087	L. chinense	1077:1087	L. chinense	1077:1087	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	6	60	located	detected	1065:1072	arg2	total					1027:1031	A total	1025:1031	A total of 13 collinear gene pairs	1025:1058	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	6	60	located	detected	1065:1072	arg1	thaliana					1105:1112	Arabidopsis thaliana	1093:1112	Arabidopsis thaliana	1093:1112	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	3	61	theme	chinense	606:613	arg1	genome					629:634	Liriodendron chinense (L. chinense) genome	593:634	Liriodendron chinense (L. chinense) genome	593:634	RESULTS In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds.
36253733	5	62	theme	Ser/Thr	867:873	arg1	structure					882:890	the same Ser/Thr kinase structure	858:890	the same Ser/Thr kinase structure in gene structure and motif composition	858:930	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	11	63	theme	LcSnRK3s	1700:1707	arg1	trend					1691:1695	The expression trend	1676:1695	The expression trend of LcSnRK3s under low-temperature stress	1676:1736	The expression trend of LcSnRK3s under low-temperature stress was mainly up-or down-regulated.
36253733	11	64	dep	down-regulated	1755:1768	arg1	up-or					1749:1753	up-or	1749:1753	up-or	1749:1753	The expression trend of LcSnRK3s under low-temperature stress was mainly up-or down-regulated.
36253733	5	65	theme	kinase	875:880	arg1	structure					882:890	the same Ser/Thr kinase structure	858:890	the same Ser/Thr kinase structure in gene structure and motif composition	858:930	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	5	66	from	structure	882:890	arg1	structure					900:908	gene structure	895:908	gene structure	895:908	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	5	66	from	structure	882:890	arg1	composition					920:930	motif composition	914:930	motif composition	914:930	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	6	67	theme	pairs	1054:1058	arg1	total					1027:1031	A total	1025:1031	A total of 13 collinear gene pairs	1025:1058	A total of 13 collinear gene pairs were detected in L. chinense and Arabidopsis thaliana (A. thaliana), and 18 pairs were detected in L. chinense and rice, suggesting that the LcSnRK family genes may be evolutionarily more closely related to rice.
36253733	0	68	theme	expression	73:82	arg1	profiles					84:91	cold stress-induced expression profiles	53:91	cold stress-induced expression profiles	53:91	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	5	69	theme	functional	943:952	arg1	domains					954:960	the functional domains	939:960	the functional domains	939:960	The LcSnRK of the three subfamilies shared the same Ser/Thr kinase structure in gene structure and motif composition, while the functional domains, except for the kinase domain, showed significant differences.
36253733	2	70	theme	stress	372:377	arg1	studies					388:394	stress response studies	372:394	stress response studies	372:394	Conducting identification and stress response studies on SnRKs in plants contributes to the development of strategies for tree species that are more tolerant to stress conditions.
36253733	0	71	theme	cold	53:56	arg1	profiles					84:91	cold stress-induced expression profiles	53:91	cold stress-induced expression profiles	53:91	Identification and characteristics of SnRK genes and cold stress-induced expression profiles in Liriodendron chinense.
36253733	3	72	theme	Liriodendron	593:604	arg1	genome					629:634	Liriodendron chinense (L. chinense) genome	593:634	Liriodendron chinense (L. chinense) genome	593:634	RESULTS In the present study, a total of 30 LcSnRKs were identified in Liriodendron chinense (L. chinense) genome, which was distributed across 15 chromosomes and 4 scaffolds.
36253733	1	73	dep	BACKGROUND	119:128	arg1	play					198:201	play	198:201	play	198:201	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36253733	1	74	theme	plant	230:234	arg1	metabolism					236:245	plant metabolism	230:245	plant metabolism	230:245	BACKGROUND The sucrose non-fermenting 1 (SNF1)-related protein kinases (SnRKs) play a vivid role in regulating plant metabolism and stress response, providing a pathway for regulation between metabolism and stress signals.
36845012	7	0	theme	transcription	1308:1320	arg1	FOS					1338:1340	FOS	1338:1340	FOS	1338:1340	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	0	theme	transcription	1308:1320	arg1	subunit					1329:1335	AP-1 transcription factor subunit	1303:1335	AP-1 transcription factor subunit (FOS)	1303:1341	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	4	1	theme	potential	744:752	arg1	factors					759:765	the potential risk factors	740:765	the potential risk factors associated with aging livers being more prone to IRI	740:818	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	7	2	theme	interaction	1607:1617	arg1	network					1619:1625	a FOS-centered interaction network	1592:1625	a FOS-centered interaction network	1592:1625	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	6	3	theme	cell	958:961	arg1	composition					963:973	immune cell composition	951:973	immune cell composition	951:973	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	3	theme	cell	958:961	arg1	Results					911:917	Results The gene expression profile and immune cell composition	911:973	Results The gene expression profile and immune cell composition between young and aging livers	911:1004	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	4	contain	had	1006:1008	arg1	Results					911:917	Results The gene expression profile and immune cell composition	911:973	Results The gene expression profile and immune cell composition between young and aging livers	911:1004	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	4	contain	had	1006:1008	arg1	composition					963:973	immune cell composition	951:973	immune cell composition	951:973	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	4	contain	had	1006:1008	arg1	profile					939:945	The gene expression profile	919:945	The gene expression profile	919:945	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	4	contain	had	1006:1008	arg2	differences					1022:1032	significant differences	1010:1032	significant differences	1010:1032	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	10	5	theme	FOS	2024:2026	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	10	6	theme	IER3	2018:2021	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	10	7	theme	liver	1870:1874	arg1	tissues					1876:1882	liver tissues	1870:1882	liver tissues	1870:1882	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	7	8	theme	Fos	1283:1285	arg1	proto-oncogene					1287:1300	Fos proto-oncogene	1283:1300	Fos proto-oncogene	1283:1300	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	9	theme	early	1258:1262	arg1	response					1264:1271	immediate early response 3	1248:1273	immediate early response 3 (IER3)	1248:1280	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	9	theme	early	1258:1262	arg1	IER3					1276:1279	IER3	1276:1279	IER3	1276:1279	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	10	theme	motif	1183:1187	arg1	ligand					1199:1204	C-X-C motif chemokine ligand 10	1177:1207	C-X-C motif chemokine ligand 10 (CXCL10)	1177:1216	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	10	theme	motif	1183:1187	arg1	CXCL10					1210:1215	CXCL10	1210:1215	CXCL10	1210:1215	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	10	11	theme	dendritic	2064:2072	arg1	cells					2074:2078	dendritic cells	2064:2078	dendritic cells	2064:2078	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	6	12	theme	significant	1010:1020	arg1	differences					1022:1032	significant differences	1010:1032	significant differences	1010:1032	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	10	13	theme	first	1930:1934	arg1	time					1936:1939	the first time	1926:1939	the first time	1926:1939	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	10	14	dep	Conclusions	1809:1819	arg1	combined					1824:1831	combined	1824:1831	combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI	1824:2138	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	6	15	theme	gene	923:926	arg1	Results					911:917	Results The gene expression profile and immune cell composition	911:973	Results The gene expression profile and immune cell composition between young and aging livers	911:1004	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	15	theme	gene	923:926	arg1	profile					939:945	The gene expression profile	919:945	The gene expression profile	919:945	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	4	16	dep	datasets	558:565	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	16	dep	datasets	558:565	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	16	dep	datasets	558:565	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	2	17	theme	young	221:225	arg1	livers					227:232	young livers	221:232	young livers	221:232	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	9	18	theme	cells	1750:1754	arg1	proportion					1726:1735	the proportion	1722:1735	the proportion of dendritic cells (DCs)	1722:1760	In addition, the proportion of dendritic cells (DCs) was significantly upregulated in aging livers.
36845012	4	19	theme	aging	653:657	arg1	tissues					665:671	aging liver tissues	653:671	aging liver tissues	653:671	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	1	20	theme	aging	128:132	arg1	intensification					98:112	the intensification	94:112	the intensification of population aging, the proportion of aging livers in the donor pool	94:182	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	4	21	theme	tissues	665:671	arg1	total					631:635	a total	629:635	a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8)	629:707	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	21	theme	tissues	665:671	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	21	theme	tissues	665:671	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	21	theme	tissues	665:671	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	11	22	from	IRI	2176:2178	arg1	livers					2189:2194	aging livers	2183:2194	aging livers	2183:2194	Nadroparin may be used to mitigate IRI in aging livers by targeting FOS, and regulation of DC activity may also reduce IRI.
36845012	0	23	theme	Aging	64:68	arg1	Livers					70:75	Aging Livers	64:75	Aging Livers	64:75	Identify Key Genes Correlated to Ischemia-Reperfusion Injury in Aging Livers.
36845012	10	24	theme	aging	2103:2107	arg1	livers					2109:2114	aging livers	2103:2114	aging livers	2103:2114	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	10	25	theme	cells	2074:2078	arg1	proportion					2050:2059	the proportion	2046:2059	the proportion of dendritic cells	2046:2078	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	10	25	theme	cells	2074:2078	arg1	changes					1960:1966	the changes	1956:1966	the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1956:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	4	26	theme	young	643:647	arg1	total					631:635	a total	629:635	a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8)	629:707	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	26	theme	young	643:647	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	26	theme	young	643:647	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	26	theme	young	643:647	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	7	27	theme	nuclear	1103:1109	arg1	factor					1160:1165	BTG antiproliferation factor 2	1138:1167	BTG antiproliferation factor 2 (BTG2)	1138:1174	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	27	theme	nuclear	1103:1109	arg1	ARNTL					1130:1134	ARNTL	1130:1134	ARNTL	1130:1134	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	27	theme	nuclear	1103:1109	arg1	BTG2					1170:1173	BTG2	1170:1173	BTG2	1170:1173	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	1	28	theme	livers	159:164	arg1	aging					128:132	population aging	117:132	population aging	117:132	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	1	28	theme	livers	159:164	arg1	proportion					139:148	the proportion	135:148	the proportion of aging livers in the donor pool	135:182	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	11	29	theme	aging	2183:2187	arg1	livers					2189:2194	aging livers	2183:2194	aging livers	2183:2194	Nadroparin may be used to mitigate IRI in aging livers by targeting FOS, and regulation of DC activity may also reduce IRI.
36845012	9	30	theme	aging	1795:1799	arg1	livers					1801:1806	aging livers	1795:1806	aging livers	1795:1806	In addition, the proportion of dendritic cells (DCs) was significantly upregulated in aging livers.
36845012	7	31	theme	hydrocarbon	1082:1092	arg1	alpha					1414:1418	coactivator 1 alpha	1400:1418	coactivator 1 alpha (PPARGC1A)	1400:1429	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	31	theme	hydrocarbon	1082:1092	arg1	gamma					1393:1397	gamma	1393:1397	gamma	1393:1397	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	31	theme	hydrocarbon	1082:1092	arg1	receptor					1094:1101	aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor	1077:1390	aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor	1077:1390	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	1	32	from	proportion	139:148	arg1	pool					179:182	the donor pool	169:182	the donor pool	169:182	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	7	33	theme	metabolism	1489:1498	arg1	regulation					1455:1464	the regulation	1451:1464	the regulation of cell proliferation, metabolism, and inflammation	1451:1516	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	34	theme	activated	1373:1381	arg1	receptor					1383:1390	peroxisome proliferative activated receptor	1348:1390	peroxisome proliferative activated receptor	1348:1390	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	0	35	from	Injury	54:59	arg1	Livers					70:75	Aging Livers	64:75	Aging Livers	64:75	Identify Key Genes Correlated to Ischemia-Reperfusion Injury in Aging Livers.
36845012	2	36	theme	aging	382:386	arg1	livers					388:393	aging livers	382:393	aging livers	382:393	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	7	37	theme	proliferation	1474:1486	arg1	regulation					1455:1464	the regulation	1451:1464	the regulation of cell proliferation, metabolism, and inflammation	1451:1516	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	4	38	theme	human	676:680	arg1	young					643:647	young	643:647	young	643:647	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	3	39	theme	risk	410:413	arg1	factors					415:421	The potential risk factors	396:421	The potential risk factors associated with IRI in aging livers	396:457	The potential risk factors associated with IRI in aging livers have not been fully elucidated.
36845012	7	40	theme	expressed	1060:1068	arg1	genes					1070:1074	the differentially expressed genes	1041:1074	the differentially expressed genes	1041:1074	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	41	theme	chemokine	1189:1197	arg1	ligand					1199:1204	C-X-C motif chemokine ligand 10	1177:1207	C-X-C motif chemokine ligand 10 (CXCL10)	1177:1216	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	41	theme	chemokine	1189:1197	arg1	CXCL10					1210:1215	CXCL10	1210:1215	CXCL10	1210:1215	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	4	42	theme	human	518:522	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	42	theme	human	518:522	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	42	theme	human	518:522	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	7	43	dep	receptor	1094:1101	arg1	ARNTL					1130:1134	ARNTL	1130:1134	ARNTL	1130:1134	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	ligand					1199:1204	C-X-C motif chemokine ligand 10	1177:1207	C-X-C motif chemokine ligand 10 (CXCL10)	1177:1216	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	chitinase					1219:1227	chitinase 3-like 1	1219:1236	chitinase 3-like 1 (CHI3L1)	1219:1245	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	BTG2					1170:1173	BTG2	1170:1173	BTG2	1170:1173	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	subunit					1329:1335	AP-1 transcription factor subunit	1303:1335	AP-1 transcription factor subunit (FOS)	1303:1341	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	response					1264:1271	immediate early response 3	1248:1273	immediate early response 3 (IER3)	1248:1280	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	IER3					1276:1279	IER3	1276:1279	IER3	1276:1279	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	FOS					1338:1340	FOS	1338:1340	FOS	1338:1340	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	CXCL10					1210:1215	CXCL10	1210:1215	CXCL10	1210:1215	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	proto-oncogene					1287:1300	Fos proto-oncogene	1283:1300	Fos proto-oncogene	1283:1300	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	receptor					1383:1390	peroxisome proliferative activated receptor	1348:1390	peroxisome proliferative activated receptor	1348:1390	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	factor					1160:1165	BTG antiproliferation factor 2	1138:1167	BTG antiproliferation factor 2 (BTG2)	1138:1174	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	43	dep	receptor	1094:1101	arg1	CHI3L1					1239:1244	CHI3L1	1239:1244	CHI3L1	1239:1244	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	3	44	theme	aging	446:450	arg1	livers					452:457	aging livers	446:457	aging livers	446:457	The potential risk factors associated with IRI in aging livers have not been fully elucidated.
36845012	7	45	theme	C-X-C	1177:1181	arg1	ligand					1199:1204	C-X-C motif chemokine ligand 10	1177:1207	C-X-C motif chemokine ligand 10 (CXCL10)	1177:1216	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	45	theme	C-X-C	1177:1181	arg1	CXCL10					1210:1215	CXCL10	1210:1215	CXCL10	1210:1215	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	4	46	theme	tissue	530:535	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	46	theme	tissue	530:535	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	46	theme	tissue	530:535	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	5	47	used	used	841:844	arg2	Online					830:835	DrugBank Online	821:835	DrugBank Online	821:835	DrugBank Online was used to screen drugs with potential to alleviate IRI in aging livers.
36845012	5	48	theme	aging	897:901	arg1	livers					903:908	aging livers	897:908	aging livers	897:908	DrugBank Online was used to screen drugs with potential to alleviate IRI in aging livers.
36845012	11	49	theme	activity	2235:2242	arg1	regulation					2218:2227	regulation	2218:2227	regulation of DC activity	2218:2242	Nadroparin may be used to mitigate IRI in aging livers by targeting FOS, and regulation of DC activity may also reduce IRI.
36845012	2	50	theme	liver	313:317	arg1	transplantation					319:333	liver transplantation	313:333	liver transplantation	313:333	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	7	51	theme	factor	1322:1327	arg1	FOS					1338:1340	FOS	1338:1340	FOS	1338:1340	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	51	theme	factor	1322:1327	arg1	subunit					1329:1335	AP-1 transcription factor subunit	1303:1335	AP-1 transcription factor subunit (FOS)	1303:1341	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	10	52	theme	profiling	1848:1856	arg1	datasets					1858:1865	the expression profiling datasets	1833:1865	the expression profiling datasets of liver tissues and samples collected in our hospital for the first time	1833:1939	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	4	53	theme	=	704:704	arg1	N					702:702	N = 8	702:706	N = 8	702:706	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	53	theme	=	704:704	arg1	mouse					695:699	mouse	695:699	mouse (N = 8)	695:707	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	7	54	theme	AP-1	1303:1306	arg1	FOS					1338:1340	FOS	1338:1340	FOS	1338:1340	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	54	theme	AP-1	1303:1306	arg1	subunit					1329:1335	AP-1 transcription factor subunit	1303:1335	AP-1 transcription factor subunit (FOS)	1303:1341	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	55	theme	FOS-centered	1594:1605	arg1	network					1619:1625	a FOS-centered interaction network	1592:1625	a FOS-centered interaction network	1592:1625	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	56	theme	BTG	1138:1140	arg1	factor					1160:1165	BTG antiproliferation factor 2	1138:1167	BTG antiproliferation factor 2 (BTG2)	1138:1174	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	56	theme	BTG	1138:1140	arg1	ARNTL					1130:1134	ARNTL	1130:1134	ARNTL	1130:1134	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	56	theme	BTG	1138:1140	arg1	BTG2					1170:1173	BTG2	1170:1173	BTG2	1170:1173	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	57	dep	tissues	1551:1557	arg1	form					1587:1590	form	1587:1590	could form a FOS-centered interaction network	1581:1625	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	57	dep	tissues	1551:1557	arg1	suffered					1559:1566	suffered	1559:1566	suffered from IRI	1559:1575	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	5	58	with	drugs	856:860	arg1	potential					867:875	potential	867:875	potential	867:875	DrugBank Online was used to screen drugs with potential to alleviate IRI in aging livers.
36845012	6	59	theme	immune	951:956	arg1	composition					963:973	immune cell composition	951:973	immune cell composition	951:973	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	59	theme	immune	951:956	arg1	Results					911:917	Results The gene expression profile and immune cell composition	911:973	Results The gene expression profile and immune cell composition between young and aging livers	911:1004	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	10	60	theme	tissues	1876:1882	arg1	datasets					1858:1865	the expression profiling datasets	1833:1865	the expression profiling datasets of liver tissues and samples collected in our hospital for the first time	1833:1939	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	4	61	theme	profiling	548:556	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	61	theme	profiling	548:556	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	61	theme	profiling	548:556	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	2	62	theme	ischemia-reperfusion	272:291	arg1	IRI					301:303	IRI	301:303	IRI	301:303	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	2	62	theme	ischemia-reperfusion	272:291	arg1	injury					293:298	ischemia-reperfusion injury	272:298	ischemia-reperfusion injury (IRI)	272:304	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	4	63	theme	risk	754:757	arg1	factors					759:765	the potential risk factors	740:765	the potential risk factors associated with aging livers being more prone to IRI	740:818	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	64	used	used	714:717	arg2	total					631:635	a total	629:635	a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8)	629:707	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	64	used	used	714:717	arg2	Methods					491:497	Methods	491:497	Methods	491:497	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	64	used	used	714:717	arg2	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	64	used	used	714:717	arg2	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	64	used	used	714:717	arg2	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	10	65	theme	PPARGC1A	2033:2040	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	1	66	theme	donor	173:177	arg1	pool					179:182	the donor pool	169:182	the donor pool	169:182	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	7	67	theme	immediate	1248:1256	arg1	response					1264:1271	immediate early response 3	1248:1273	immediate early response 3 (IER3)	1248:1280	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	67	theme	immediate	1248:1256	arg1	IER3					1276:1279	IER3	1276:1279	IER3	1276:1279	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	11	68	theme	DC	2232:2233	arg1	activity					2235:2242	DC activity	2232:2242	DC activity	2232:2242	Nadroparin may be used to mitigate IRI in aging livers by targeting FOS, and regulation of DC activity may also reduce IRI.
36845012	10	69	from	changes	1960:1966	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	5	70	from	IRI	890:892	arg1	livers					903:908	aging livers	897:908	aging livers	897:908	DrugBank Online was used to screen drugs with potential to alleviate IRI in aging livers.
36845012	1	71	with	Background	78:87	arg1	intensification					98:112	the intensification	94:112	the intensification of population aging, the proportion of aging livers in the donor pool	94:182	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	9	72	theme	dendritic	1740:1748	arg1	DCs					1757:1759	DCs	1757:1759	DCs	1757:1759	In addition, the proportion of dendritic cells (DCs) was significantly upregulated in aging livers.
36845012	9	72	theme	dendritic	1740:1748	arg1	cells					1750:1754	dendritic cells	1740:1754	dendritic cells (DCs)	1740:1760	In addition, the proportion of dendritic cells (DCs) was significantly upregulated in aging livers.
36845012	1	73	theme	population	117:126	arg1	aging					128:132	population aging	117:132	population aging	117:132	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	1	73	theme	population	117:126	arg1	proportion					139:148	the proportion	135:148	the proportion of aging livers in the donor pool	135:182	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	7	74	theme	3-like	1229:1234	arg1	CHI3L1					1239:1244	CHI3L1	1239:1244	CHI3L1	1239:1244	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	74	theme	3-like	1229:1234	arg1	chitinase					1219:1227	chitinase 3-like 1	1219:1236	chitinase 3-like 1 (CHI3L1)	1219:1245	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	75	theme	liver	1545:1549	arg1	tissues					1551:1557	liver tissues	1545:1557	liver tissues suffered from IRI and could form a FOS-centered interaction network	1545:1625	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	76	theme	coactivator	1400:1410	arg1	PPARGC1A					1421:1428	PPARGC1A	1421:1428	PPARGC1A	1421:1428	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	76	theme	coactivator	1400:1410	arg1	alpha					1414:1418	coactivator 1 alpha	1400:1418	coactivator 1 alpha (PPARGC1A)	1400:1429	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	76	theme	coactivator	1400:1410	arg1	receptor					1094:1101	aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor	1077:1390	aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor	1077:1390	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	4	77	theme	aging	783:787	arg1	livers					789:794	aging livers	783:794	aging livers	783:794	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	78	theme	liver	659:663	arg1	tissues					665:671	aging liver tissues	653:671	aging liver tissues	653:671	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	7	79	theme	translocator-like	1111:1127	arg1	factor					1160:1165	BTG antiproliferation factor 2	1138:1167	BTG antiproliferation factor 2 (BTG2)	1138:1174	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	79	theme	translocator-like	1111:1127	arg1	ARNTL					1130:1134	ARNTL	1130:1134	ARNTL	1130:1134	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	79	theme	translocator-like	1111:1127	arg1	BTG2					1170:1173	BTG2	1170:1173	BTG2	1170:1173	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	2	80	theme	aging	235:239	arg1	livers					241:246	aging livers	235:246	aging livers	235:246	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	1	81	theme	aging	153:157	arg1	livers					159:164	aging livers	153:164	aging livers	153:164	Background With the intensification of population aging, the proportion of aging livers in the donor pool is increasing rapidly.
36845012	10	82	theme	BTG2	1996:1999	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	7	83	theme	aryl	1077:1080	arg1	alpha					1414:1418	coactivator 1 alpha	1400:1418	coactivator 1 alpha (PPARGC1A)	1400:1429	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	83	theme	aryl	1077:1080	arg1	gamma					1393:1397	gamma	1393:1397	gamma	1393:1397	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	83	theme	aryl	1077:1080	arg1	receptor					1094:1101	aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor	1077:1390	aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor	1077:1390	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	4	84	theme	mouse	695:699	arg1	young					643:647	young	643:647	young	643:647	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	7	85	theme	inflammation	1505:1516	arg1	regulation					1455:1464	the regulation	1451:1464	the regulation of cell proliferation, metabolism, and inflammation	1451:1516	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	86	theme	proliferative	1359:1371	arg1	receptor					1383:1390	peroxisome proliferative activated receptor	1348:1390	peroxisome proliferative activated receptor	1348:1390	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	10	87	theme	ARNTL	1989:1993	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	4	88	theme	=	685:685	arg1	N					683:683	N = 20	683:688	N = 20	683:688	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	88	theme	=	685:685	arg1	human					676:680	human	676:680	human	676:680	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	11	89	used	used	2159:2162	arg2	Nadroparin					2141:2150	Nadroparin	2141:2150	Nadroparin	2141:2150	Nadroparin may be used to mitigate IRI in aging livers by targeting FOS, and regulation of DC activity may also reduce IRI.
36845012	10	90	theme	CHI3L1	2010:2015	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	6	91	dep	Results	911:917	arg1	composition					963:973	immune cell composition	951:973	immune cell composition	951:973	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	91	dep	Results	911:917	arg1	Results					911:917	Results The gene expression profile and immune cell composition	911:973	Results The gene expression profile and immune cell composition between young and aging livers	911:1004	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	91	dep	Results	911:917	arg1	profile					939:945	The gene expression profile	919:945	The gene expression profile	919:945	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	0	92	theme	Key	9:11	arg1	Genes					13:17	Key Genes	9:17	Key Genes Correlated to Ischemia-Reperfusion Injury in Aging Livers	9:75	Identify Key Genes Correlated to Ischemia-Reperfusion Injury in Aging Livers.
36845012	6	93	theme	expression	928:937	arg1	Results					911:917	Results The gene expression profile and immune cell composition	911:973	Results The gene expression profile and immune cell composition between young and aging livers	911:1004	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	6	93	theme	expression	928:937	arg1	profile					939:945	The gene expression profile	919:945	The gene expression profile	919:945	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	4	94	theme	liver	524:528	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	94	theme	liver	524:528	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	94	theme	liver	524:528	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	10	95	theme	CXCL10	2002:2007	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	2	96	theme	utilization	362:372	arg1	rate					374:377	the utilization rate	358:377	the utilization rate of aging livers	358:393	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	0	97	theme	Correlated	19:28	arg1	Genes					13:17	Key Genes	9:17	Key Genes Correlated to Ischemia-Reperfusion Injury in Aging Livers	9:75	Identify Key Genes Correlated to Ischemia-Reperfusion Injury in Aging Livers.
36845012	5	98	theme	DrugBank	821:828	arg1	Online					830:835	DrugBank Online	821:835	DrugBank Online	821:835	DrugBank Online was used to screen drugs with potential to alleviate IRI in aging livers.
36845012	7	99	theme	cell	1469:1472	arg1	proliferation					1474:1486	cell proliferation	1469:1486	cell proliferation	1469:1486	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	4	100	theme	expression	537:546	arg1	datasets					558:565	five human liver tissue expression profiling datasets	513:565	five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648)	513:623	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	100	theme	expression	537:546	arg1	GSE151648					614:622	GSE151648	614:622	GSE151648	614:622	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	4	100	theme	expression	537:546	arg1	GSE133815					599:607	GSE133815	599:607	GSE133815	599:607	Methods In this work, five human liver tissue expression profiling datasets (GSE61260, GSE107037, GSE89632, GSE133815, and GSE151648) and a total of 28 young and aging liver tissues of human (N = 20) and mouse (N = 8) were used to screen and verify the potential risk factors associated with aging livers being more prone to IRI.
36845012	0	101	theme	Ischemia-Reperfusion	33:52	arg1	Injury					54:59	Ischemia-Reperfusion Injury	33:59	Ischemia-Reperfusion Injury in Aging Livers	33:75	Identify Key Genes Correlated to Ischemia-Reperfusion Injury in Aging Livers.
36845012	7	102	theme	peroxisome	1348:1357	arg1	receptor					1383:1390	peroxisome proliferative activated receptor	1348:1390	peroxisome proliferative activated receptor	1348:1390	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	3	103	theme	potential	400:408	arg1	factors					415:421	The potential risk factors	396:421	The potential risk factors associated with IRI in aging livers	396:457	The potential risk factors associated with IRI in aging livers have not been fully elucidated.
36845012	3	104	from	IRI	439:441	arg1	livers					452:457	aging livers	446:457	aging livers	446:457	The potential risk factors associated with IRI in aging livers have not been fully elucidated.
36845012	2	105	theme	livers	388:393	arg1	rate					374:377	the utilization rate	358:377	the utilization rate of aging livers	358:393	Compared with young livers, aging livers are more susceptible to ischemia-reperfusion injury (IRI) during liver transplantation, which greatly affects the utilization rate of aging livers.
36845012	7	106	theme	antiproliferation	1142:1158	arg1	factor					1160:1165	BTG antiproliferation factor 2	1138:1167	BTG antiproliferation factor 2 (BTG2)	1138:1174	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	106	theme	antiproliferation	1142:1158	arg1	ARNTL					1130:1134	ARNTL	1130:1134	ARNTL	1130:1134	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	7	106	theme	antiproliferation	1142:1158	arg1	BTG2					1170:1173	BTG2	1170:1173	BTG2	1170:1173	Among the differentially expressed genes, aryl hydrocarbon receptor nuclear translocator-like (ARNTL), BTG antiproliferation factor 2 (BTG2), C-X-C motif chemokine ligand 10 (CXCL10), chitinase 3-like 1 (CHI3L1), immediate early response 3 (IER3), Fos proto-oncogene, AP-1 transcription factor subunit (FOS), and peroxisome proliferative activated receptor, gamma, coactivator 1 alpha (PPARGC1A), mainly involved in the regulation of cell proliferation, metabolism, and inflammation, were also dysregulated in liver tissues suffered from IRI and could form a FOS-centered interaction network.
36845012	10	107	from	proportion	2050:2059	arg1	expression					1975:1984	the expression	1971:1984	the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A	1971:2040	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	6	108	dep	young	983:987	arg1	livers					999:1004	livers	999:1004	livers	999:1004	Results The gene expression profile and immune cell composition between young and aging livers had significant differences.
36845012	10	109	theme	samples	1888:1894	arg1	datasets					1858:1865	the expression profiling datasets	1833:1865	the expression profiling datasets of liver tissues and samples collected in our hospital for the first time	1833:1939	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
36845012	10	110	theme	expression	1837:1846	arg1	datasets					1858:1865	the expression profiling datasets	1833:1865	the expression profiling datasets of liver tissues and samples collected in our hospital for the first time	1833:1939	Conclusions We combined the expression profiling datasets of liver tissues and samples collected in our hospital for the first time to reveal that the changes in the expression of ARNTL, BTG2, CXCL10, CHI3L1, IER3, FOS, and PPARGC1A and the proportion of dendritic cells may be associated with aging livers being more prone to IRI.
35586929	10	0	theme	microbiota	1447:1456	arg1	composition					1458:1468	gut microbiota composition	1443:1468	gut microbiota composition	1443:1468	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	6	1	theme	Sow	838:840	arg1	samples					859:865	Sow and piglet fecal samples	838:865	Sow and piglet fecal samples	838:865	Sow and piglet fecal samples were collected on days 7 and 28 after farrowing.
35586929	10	2	theme	host	1417:1420	arg1	outcomes					1430:1437	better host fitness outcomes	1410:1437	better host fitness outcomes	1410:1437	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	4	3	theme	sows	651:654	arg1	composition					626:636	gut microbiota composition	611:636	gut microbiota composition of lactating sows and their litters	611:672	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	0	4	theme	Sows	78:81	arg1	Composition					53:63	Fecal Microbiome Composition	36:63	Fecal Microbiome Composition of Lactating Sows and Their Piglets	36:99	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	9	5	from	day	1287:1289	arg1	proportion					1254:1263	a significantly reduced proportion	1230:1263	a significantly reduced proportion in MC-treated sows at day 28	1230:1292	Clostridium and Spirochaetaceae showed a significantly reduced proportion in MC-treated sows at day 28.
35586929	9	5	from	day	1287:1289	arg1	sows					1279:1282	MC-treated sows	1268:1282	MC-treated sows at day 28	1268:1292	Clostridium and Spirochaetaceae showed a significantly reduced proportion in MC-treated sows at day 28.
35586929	1	6	theme	pork	151:154	arg1	industry					156:163	the pork industry	147:163	the pork industry	147:163	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	10	7	theme	carbohydrases	1349:1361	arg1	link					1373:1376	their link	1367:1376	their link with improved production due to better host fitness outcomes and gut microbiota composition	1367:1468	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	10	7	theme	carbohydrases	1349:1361	arg1	effects					1330:1336	the beneficial effects	1315:1336	the beneficial effects of dietary carbohydrases	1315:1361	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	0	8	theme	Piglets	93:99	arg1	Composition					53:63	Fecal Microbiome Composition	36:63	Fecal Microbiome Composition of Lactating Sows and Their Piglets	36:99	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	0	9	from	Effects	0:6	arg1	Composition					53:63	Fecal Microbiome Composition	36:63	Fecal Microbiome Composition of Lactating Sows and Their Piglets	36:99	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	10	10	theme	dietary	1341:1347	arg1	carbohydrases					1349:1361	dietary carbohydrases	1341:1361	dietary carbohydrases	1341:1361	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	1	11	theme	Corn-soybean	102:113	arg1	diets					120:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	1	11	theme	Corn-soybean	102:113	arg1	source					178:183	a primary source	168:183	a primary source of energy and protein	168:205	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	7	12	from	sequencing	965:974	arg1	results					929:935	the results	925:935	the results from 16S rRNA gene amplicon sequencing	925:974	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	5	13	theme	experimental	679:690	arg1	design					692:697	The experimental design	675:697	The experimental design	675:697	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	14	contain	contained	699:707	arg1	design					692:697	The experimental design	675:697	The experimental design	675:697	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	14	contain	contained	699:707	arg2	treatments					721:730	two dietary treatments	709:730	two dietary treatments	709:730	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	14	contain	contained	699:707	arg2	CON					778:780	CON	778:780	CON supplemented with 0.01% multigrain carbohydrases (MCs)	778:835	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	14	contain	contained	699:707	arg2	diet					735:738	a diet	733:738	a diet based on corn-soybean meal (CON)	733:771	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	4	15	theme	lactating	641:649	arg1	sows					651:654	lactating sows	641:654	lactating sows	641:654	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	9	16	from	proportion	1254:1263	arg1	sows					1279:1282	MC-treated sows	1268:1282	MC-treated sows at day 28	1268:1292	Clostridium and Spirochaetaceae showed a significantly reduced proportion in MC-treated sows at day 28.
35586929	9	16	from	proportion	1254:1263	arg1	day					1287:1289	day 28	1287:1292	day 28	1287:1292	Clostridium and Spirochaetaceae showed a significantly reduced proportion in MC-treated sows at day 28.
35586929	2	17	theme	non-starch	248:257	arg1	polysaccharides					259:273	non-starch polysaccharides	248:273	non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility	248:370	However, such a diet generally contains non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility.
35586929	2	17	theme	non-starch	248:257	arg1	NSPs					276:279	NSPs	276:279	NSPs	276:279	However, such a diet generally contains non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility.
35586929	5	18	theme	0.01	800:803	arg1	%					804:804	%	804:804	%	804:804	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	8	19	theme	MC	1110:1111	arg1	treatment					1113:1121	the MC treatment	1106:1121	the MC treatment	1106:1121	Specifically, the MC treatment induced an increase in the proportions of Lactobacillus in piglets.
35586929	7	20	theme	microbial	1032:1040	arg1	compositions					1042:1053	the microbial compositions	1028:1053	the microbial compositions	1028:1053	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	3	21	theme	growth	495:500	arg1	performance					502:512	improved growth performance	486:512	improved growth performance	486:512	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	10	22	with	effects	1330:1336	arg1	production					1392:1401	improved production	1383:1401	improved production due to better host fitness outcomes and gut microbiota composition	1383:1468	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	5	23	theme	corn-soybean	749:760	arg1	meal					762:765	corn-soybean meal	749:765	corn-soybean meal (CON)	749:771	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	23	theme	corn-soybean	749:760	arg1	CON					768:770	CON	768:770	CON	768:770	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	10	24	theme	gut	1443:1445	arg1	composition					1458:1468	gut microbiota composition	1443:1468	gut microbiota composition	1443:1468	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	4	25	from	effects	575:581	arg1	composition					626:636	gut microbiota composition	611:636	gut microbiota composition of lactating sows and their litters	611:672	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	4	25	from	effects	575:581	arg1	diets					602:606	lactation diets	592:606	lactation diets	592:606	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	4	26	theme	microbiota	615:624	arg1	composition					626:636	gut microbiota composition	611:636	gut microbiota composition of lactating sows and their litters	611:672	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	7	27	dep	species	998:1004	arg1	diversity					1006:1014	diversity	1006:1014	species diversity	998:1014	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	2	28	contain	contains	239:246	arg1	diet					224:227	such a diet	217:227	such a diet	217:227	However, such a diet generally contains non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility.
35586929	2	28	contain	contains	239:246	arg2	NSPs					276:279	NSPs	276:279	NSPs	276:279	However, such a diet generally contains non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility.
35586929	2	28	contain	contains	239:246	arg2	polysaccharides					259:273	non-starch polysaccharides	248:273	non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility	248:370	However, such a diet generally contains non-starch polysaccharides (NSPs) which present a challenge in finding ways to improve their availability and digestibility.
35586929	10	29	theme	improved	1383:1390	arg1	production					1392:1401	improved production	1383:1401	improved production due to better host fitness outcomes and gut microbiota composition	1383:1468	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	10	30	with	link	1373:1376	arg1	production					1392:1401	improved production	1383:1401	improved production due to better host fitness outcomes and gut microbiota composition	1383:1468	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	1	31	theme	primary	170:176	arg1	diets					120:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	1	31	theme	primary	170:176	arg1	source					178:183	a primary source	168:183	a primary source of energy and protein	168:205	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	0	32	theme	Carbohydrases	19:31	arg1	Effects					0:6	Effects	0:6	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.	0:100	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	9	33	theme	reduced	1246:1252	arg1	proportion					1254:1263	a significantly reduced proportion	1230:1263	a significantly reduced proportion in MC-treated sows at day 28	1230:1292	Clostridium and Spirochaetaceae showed a significantly reduced proportion in MC-treated sows at day 28.
35586929	10	34	theme	better	1410:1415	arg1	outcomes					1430:1437	better host fitness outcomes	1410:1437	better host fitness outcomes	1410:1437	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	3	35	theme	host	518:521	arg1	fitness					534:540	host intestinal fitness	518:540	host intestinal fitness	518:540	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	3	36	theme	efficient	432:440	arg1	multi-carbohydrases					381:399	Dietary multi-carbohydrases	373:399	Dietary multi-carbohydrases (MCs)	373:405	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	3	36	theme	efficient	432:440	arg1	approach					442:449	an efficient approach	429:449	an efficient approach to utilize NSPs	429:465	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	0	37	theme	Dietary	11:17	arg1	Carbohydrases					19:31	Dietary Carbohydrases	11:31	Dietary Carbohydrases	11:31	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	3	38	theme	improved	486:493	arg1	performance					502:512	improved growth performance	486:512	improved growth performance	486:512	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	3	39	theme	intestinal	523:532	arg1	fitness					534:540	host intestinal fitness	518:540	host intestinal fitness	518:540	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	10	40	theme	beneficial	1319:1328	arg1	effects					1330:1336	the beneficial effects	1315:1336	the beneficial effects of dietary carbohydrases	1315:1361	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	0	41	theme	Fecal	36:40	arg1	Composition					53:63	Fecal Microbiome Composition	36:63	Fecal Microbiome Composition of Lactating Sows and Their Piglets	36:99	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	7	42	from	changes	987:993	arg1	species					998:1004	species diversity	998:1014	species diversity	998:1014	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	7	43	theme	gene	951:954	arg1	sequencing					965:974	16S rRNA gene amplicon sequencing	942:974	16S rRNA gene amplicon sequencing	942:974	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	4	44	theme	lactation	592:600	arg1	diets					602:606	lactation diets	592:606	lactation diets	592:606	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	4	45	theme	gut	611:613	arg1	composition					626:636	gut microbiota composition	611:636	gut microbiota composition of lactating sows and their litters	611:672	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	1	46	theme	energy	188:193	arg1	diets					120:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	1	46	theme	energy	188:193	arg1	source					178:183	a primary source	168:183	a primary source of energy and protein	168:205	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	9	47	theme	MC-treated	1268:1277	arg1	sows					1279:1282	MC-treated sows	1268:1282	MC-treated sows at day 28	1268:1292	Clostridium and Spirochaetaceae showed a significantly reduced proportion in MC-treated sows at day 28.
35586929	7	48	theme	amplicon	956:963	arg1	sequencing					965:974	16S rRNA gene amplicon sequencing	942:974	16S rRNA gene amplicon sequencing	942:974	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	8	49	theme	Lactobacillus	1165:1177	arg1	proportions					1150:1160	the proportions	1146:1160	the proportions of Lactobacillus in piglets	1146:1188	Specifically, the MC treatment induced an increase in the proportions of Lactobacillus in piglets.
35586929	3	50	theme	Dietary	373:379	arg1	multi-carbohydrases					381:399	Dietary multi-carbohydrases	373:399	Dietary multi-carbohydrases (MCs)	373:405	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	3	50	theme	Dietary	373:379	arg1	approach					442:449	an efficient approach	429:449	an efficient approach to utilize NSPs	429:465	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	3	50	theme	Dietary	373:379	arg1	MCs					402:404	MCs	402:404	MCs	402:404	Dietary multi-carbohydrases (MCs) have been proposed as an efficient approach to utilize NSPs, and can result in improved growth performance and host intestinal fitness.
35586929	7	51	theme	16S	942:944	arg1	sequencing					965:974	16S rRNA gene amplicon sequencing	942:974	16S rRNA gene amplicon sequencing	942:974	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	8	52	from	increase	1134:1141	arg1	proportions					1150:1160	the proportions	1146:1160	the proportions of Lactobacillus in piglets	1146:1188	Specifically, the MC treatment induced an increase in the proportions of Lactobacillus in piglets.
35586929	1	53	theme	meal	115:118	arg1	diets					120:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	1	53	theme	meal	115:118	arg1	source					178:183	a primary source	168:183	a primary source of energy and protein	168:205	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	0	54	theme	Microbiome	42:51	arg1	Composition					53:63	Fecal Microbiome Composition	36:63	Fecal Microbiome Composition of Lactating Sows and Their Piglets	36:99	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	6	55	theme	fecal	853:857	arg1	samples					859:865	Sow and piglet fecal samples	838:865	Sow and piglet fecal samples	838:865	Sow and piglet fecal samples were collected on days 7 and 28 after farrowing.
35586929	7	56	theme	rRNA	946:949	arg1	sequencing					965:974	16S rRNA gene amplicon sequencing	942:974	16S rRNA gene amplicon sequencing	942:974	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	6	57	theme	piglet	846:851	arg1	samples					859:865	Sow and piglet fecal samples	838:865	Sow and piglet fecal samples	838:865	Sow and piglet fecal samples were collected on days 7 and 28 after farrowing.
35586929	10	58	theme	fitness	1422:1428	arg1	outcomes					1430:1437	better host fitness outcomes	1410:1437	better host fitness outcomes	1410:1437	Our results support the beneficial effects of dietary carbohydrases and their link with improved production due to better host fitness outcomes and gut microbiota composition.
35586929	5	59	theme	%	804:804	arg1	MCs					832:834	MCs	832:834	MCs	832:834	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	59	theme	%	804:804	arg1	carbohydrases					817:829	0.01% multigrain carbohydrases	800:829	0.01% multigrain carbohydrases (MCs)	800:835	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	1	60	theme	protein	199:205	arg1	diets					120:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	1	60	theme	protein	199:205	arg1	source					178:183	a primary source	168:183	a primary source of energy and protein	168:205	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	1	61	used	used	139:142	arg2	source					178:183	a primary source	168:183	a primary source of energy and protein	168:205	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	1	61	used	used	139:142	arg2	diets					120:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets	102:124	Corn-soybean meal diets are commonly used in the pork industry as a primary source of energy and protein.
35586929	4	62	theme	litters	666:672	arg1	composition					626:636	gut microbiota composition	611:636	gut microbiota composition of lactating sows and their litters	611:672	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	0	63	theme	Lactating	68:76	arg1	Sows					78:81	Lactating Sows	68:81	Lactating Sows	68:81	Effects of Dietary Carbohydrases on Fecal Microbiome Composition of Lactating Sows and Their Piglets.
35586929	4	64	theme	MC	586:587	arg1	effects					575:581	the effects	571:581	the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters	571:672	In this study, we evaluated the effects of MC in lactation diets on gut microbiota composition of lactating sows and their litters.
35586929	5	65	theme	multigrain	806:815	arg1	MCs					832:834	MCs	832:834	MCs	832:834	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	65	theme	multigrain	806:815	arg1	carbohydrases					817:829	0.01% multigrain carbohydrases	800:829	0.01% multigrain carbohydrases (MCs)	800:835	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	5	66	theme	dietary	713:719	arg1	treatments					721:730	two dietary treatments	709:730	two dietary treatments	709:730	The experimental design contained two dietary treatments, a diet based on corn-soybean meal (CON), and CON supplemented with 0.01% multigrain carbohydrases (MCs).
35586929	7	67	theme	lactating	1058:1066	arg1	sows					1068:1071	lactating sows	1058:1071	lactating sows	1058:1071	Based on the results from 16S rRNA gene amplicon sequencing, MC led to changes in species diversity and altered the microbial compositions in lactating sows and their piglets.
35586929	8	68	from	proportions	1150:1160	arg1	piglets					1182:1188	piglets	1182:1188	piglets	1182:1188	Specifically, the MC treatment induced an increase in the proportions of Lactobacillus in piglets.
35020682	7	0	dep	0.78-1.05	1443:1451	arg1	.19					1458:1460	P = .19	1454:1460	P = .19	1454:1460	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	3	1	theme	score-matched	566:578	arg1	study					594:598	a propensity score-matched retrospective study	553:598	a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	553:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	7	2	theme	%	1415:1415	arg1	interval					1428:1435	95% confidence interval [CI	1413:1439	95% confidence interval [CI	1413:1439	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	2	3	theme	NMB	458:460	arg1	reversal					462:469	NMB reversal	458:469	NMB reversal	458:469	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	5	4	theme	postoperative	888:900	arg1	occurrence					950:959	the occurrence	946:959	the occurrence of either postoperative pneumonia or respiratory failure	946:1016	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	5	4	theme	postoperative	888:900	arg1	complication					912:923	major postoperative pulmonary complication	882:923	major postoperative pulmonary complication	882:923	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	1	5	from	reduction	280:288	arg1	risk					297:300	the risk	293:300	the risk of composite postoperative pulmonary complications	293:351	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	9	6	theme	CONCLUSIONS	1625:1635	arg1	Choice					1637:1642	CONCLUSIONS Choice	1625:1642	CONCLUSIONS Choice of NMB reversal agent	1625:1664	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	2	7	from	significance	375:386	arg1	children					418:425	children	418:425	children	418:425	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	5	8	theme	postoperative	971:983	arg1	pneumonia					985:993	postoperative pneumonia	971:993	postoperative pneumonia	971:993	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	10	9	theme	chronic	1903:1909	arg1	conditions					1911:1920	complex chronic conditions	1895:1920	complex chronic conditions	1895:1920	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	5	10	theme	primary	862:868	arg1	outcome					870:876	Our primary outcome	858:876	Our primary outcome	858:876	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	3	11	theme	System	639:644	arg1	dataset					653:659	the Pediatric Health Information System (PHIS) dataset	606:659	the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	606:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	1	12	theme	neuromuscular	220:232	arg1	NMB					241:243	NMB	241:243	NMB	241:243	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	1	12	theme	neuromuscular	220:232	arg1	block					234:238	neuromuscular block	220:238	neuromuscular block (NMB)	220:244	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	9	13	theme	reversal	1651:1658	arg1	agent					1660:1664	NMB reversal agent	1647:1664	NMB reversal agent	1647:1664	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	10	14	theme	anesthesia	1927:1936	arg1	technique					1938:1946	anesthesia technique	1927:1946	anesthesia technique	1927:1946	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	3	15	theme	Health	620:625	arg1	dataset					653:659	the Pediatric Health Information System (PHIS) dataset	606:659	the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	606:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	1	16	theme	adult	129:133	arg1	patients					135:142	adult patients	129:142	adult patients	129:142	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	7	17	theme	pulmonary	1351:1359	arg1	complications					1361:1373	pulmonary complications	1351:1373	pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI]	1351:1440	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	7	17	theme	pulmonary	1351:1359	arg1	%					1379:1379	3.1%	1376:1379	3.1%	1376:1379	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	7	17	theme	pulmonary	1351:1359	arg1	%					1387:1387	3.1%	1384:1387	3.1%	1384:1387	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	7	17	theme	pulmonary	1351:1359	arg1	0.78-1.05					1443:1451	0.78-1.05	1443:1451	0.78-1.05	1443:1451	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	5	18	theme	pneumonia	985:993	arg1	occurrence					950:959	the occurrence	946:959	the occurrence of either postoperative pneumonia or respiratory failure	946:1016	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	5	18	theme	pneumonia	985:993	arg1	complication					912:923	major postoperative pulmonary complication	882:923	major postoperative pulmonary complication	882:923	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	0	19	theme	Pulmonary	66:74	arg1	Complications					76:88	Major Postoperative Pulmonary Complications	46:88	Major Postoperative Pulmonary Complications in Children	46:100	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children.
35020682	1	20	theme	pulmonary	329:337	arg1	complications					339:351	composite postoperative pulmonary complications	305:351	composite postoperative pulmonary complications	305:351	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	0	21	theme	Sugammadex	15:24	arg1	Association					0:10	Association	0:10	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children	0:100	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children.
35020682	6	22	theme	study	1048:1052	arg1	population					1054:1063	a study population	1046:1063	a study population	1046:1063	RESULTS Our study included a study population of 33,819 children, of whom 23,312 (68.9%) received neostigmine and 10,507 (31.1%) received sugammadex.
35020682	2	23	theme	complications	492:504	arg1	risk					478:481	the risk	474:481	the risk of these complications	474:504	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	2	24	from	role	450:453	arg1	risk					478:481	the risk	474:481	the risk of these complications	474:504	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	7	25	dep	odds	1390:1393	arg1	ratio					1395:1399	ratio	1395:1399	odds ratio [OR	1390:1403	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	7	26	dep	score	1186:1190	arg1	10,361					1202:1207	10,361	1202:1207	10,361 matched from each group	1202:1231	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	5	27	theme	failure	1010:1016	arg1	occurrence					950:959	the occurrence	946:959	the occurrence of either postoperative pneumonia or respiratory failure	946:1016	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	5	27	theme	failure	1010:1016	arg1	complication					912:923	major postoperative pulmonary complication	882:923	major postoperative pulmonary complication	882:923	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	9	28	theme	major	1709:1713	arg1	complications					1739:1751	major postoperative pulmonary complications	1709:1751	major postoperative pulmonary complications	1709:1751	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	9	29	theme	pulmonary	1729:1737	arg1	complications					1739:1751	major postoperative pulmonary complications	1709:1751	major postoperative pulmonary complications	1709:1751	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	2	30	theme	complications	401:413	arg1	significance					375:386	the clinical significance	362:386	the clinical significance of pulmonary complications in children	362:425	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	7	31	theme	matching	1192:1199	arg1	score					1186:1190	propensity score matching	1175:1199	propensity score matching (10,361 matched from each group)	1175:1232	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	1	32	theme	significant	268:278	arg1	reduction					280:288	a significant reduction	266:288	a significant reduction in the risk of composite postoperative pulmonary complications	266:351	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	1	33	theme	BACKGROUND	103:112	arg1	data					121:124	BACKGROUND Recent data	103:124	BACKGROUND Recent data in adult patients	103:142	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	4	34	theme	surgical	771:778	arg1	procedures					780:789	elective, inpatient, noncardiac surgical procedures	739:789	elective, inpatient, noncardiac surgical procedures	739:789	We studied children <18 years who underwent elective, inpatient, noncardiac surgical procedures and received either neostigmine or sugammadex for reversal of NMB.
35020682	7	35	theme	propensity	1175:1184	arg1	score					1186:1190	propensity score matching	1175:1199	propensity score matching (10,361 matched from each group)	1175:1232	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	7	36	theme	NMB	1311:1313	arg1	agent					1324:1328	the NMB reversal agent	1307:1328	the NMB reversal agent	1307:1328	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	5	37	theme	major	882:886	arg1	occurrence					950:959	the occurrence	946:959	the occurrence of either postoperative pneumonia or respiratory failure	946:1016	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	5	37	theme	major	882:886	arg1	complication					912:923	major postoperative pulmonary complication	882:923	major postoperative pulmonary complication	882:923	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	7	38	dep	0.90	1407:1410	arg1	interval					1428:1435	95% confidence interval [CI	1413:1439	95% confidence interval [CI	1413:1439	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	0	39	theme	Neostigmine	29:39	arg1	Association					0:10	Association	0:10	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children	0:100	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children.
35020682	7	40	theme	=	1456:1456	arg1	.19					1458:1460	P = .19	1454:1460	P = .19	1454:1460	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	6	41	theme	children	1075:1082	arg1	population					1054:1063	a study population	1046:1063	a study population	1046:1063	RESULTS Our study included a study population of 33,819 children, of whom 23,312 (68.9%) received neostigmine and 10,507 (31.1%) received sugammadex.
35020682	8	42	theme	complications	1492:1504	arg1	pneumonia					1541:1549	pneumonia	1541:1549	pneumonia	1541:1549	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
35020682	8	42	theme	complications	1492:1504	arg1	failure					1529:1535	respiratory failure	1517:1535	respiratory failure	1517:1535	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
35020682	8	42	theme	complications	1492:1504	arg1	components					1468:1477	The components	1464:1477	The components	1464:1477	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
35020682	0	43	theme	Major	46:50	arg1	Complications					76:88	Major Postoperative Pulmonary Complications	46:88	Major Postoperative Pulmonary Complications in Children	46:100	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children.
35020682	1	44	theme	composite	305:313	arg1	complications					339:351	composite postoperative pulmonary complications	305:351	composite postoperative pulmonary complications	305:351	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	7	45	theme	significant	1275:1285	arg1	association					1287:1297	a statistically significant association	1259:1297	a statistically significant association between the NMB reversal agent	1259:1328	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	3	46	theme	PHIS	647:650	arg1	dataset					653:659	the Pediatric Health Information System (PHIS) dataset	606:659	the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	606:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	0	47	with	Association	0:10	arg1	Complications					76:88	Major Postoperative Pulmonary Complications	46:88	Major Postoperative Pulmonary Complications in Children	46:100	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children.
35020682	1	48	from	data	121:124	arg1	patients					135:142	adult patients	129:142	adult patients	129:142	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	1	49	theme	block	234:238	arg1	reversal					208:215	reversal	208:215	reversal of neuromuscular block (NMB)	208:244	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	3	50	dep	years	674:678	arg1	2020					689:692	2020	689:692	2020	689:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	3	50	dep	years	674:678	arg1	2016					680:683	2016	680:683	2016	680:683	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	3	51	dep	METHOD	533:538	arg1	performed					543:551	performed	543:551	performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	543:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	10	52	contain	carry	1814:1818	arg2	forth					1820:1824	forth	1820:1824	forth	1820:1824	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	10	52	contain	carry	1814:1818	arg1	results					1806:1812	our results	1802:1812	our results	1802:1812	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	10	53	theme	surgical	1859:1866	arg1	specialty					1868:1876	surgical specialty	1859:1876	surgical specialty	1859:1876	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	2	54	theme	clinical	366:373	arg1	significance					375:386	the clinical significance	362:386	the clinical significance of pulmonary complications in children	362:425	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	8	55	theme	NMB	1605:1607	arg1	agent					1618:1622	NMB reversal agent	1605:1622	NMB reversal agent	1605:1622	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
35020682	7	56	theme	confidence	1417:1426	arg1	interval					1428:1435	95% confidence interval [CI	1413:1439	95% confidence interval [CI	1413:1439	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	3	57	theme	propensity	555:564	arg1	study					594:598	a propensity score-matched retrospective study	553:598	a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	553:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	8	58	theme	agent	1618:1622	arg1	choice					1595:1600	the choice	1591:1600	the choice of NMB reversal agent	1591:1622	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
35020682	5	59	theme	pulmonary	902:910	arg1	occurrence					950:959	the occurrence	946:959	the occurrence of either postoperative pneumonia or respiratory failure	946:1016	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	5	59	theme	pulmonary	902:910	arg1	complication					912:923	major postoperative pulmonary complication	882:923	major postoperative pulmonary complication	882:923	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	1	60	theme	sugammadex	169:178	arg1	use					162:164	the use	158:164	the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB)	158:244	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	7	61	dep	%	1379:1379	arg1	odds					1390:1393	odds ratio [OR	1390:1403	odds ratio [OR	1390:1403	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	10	62	theme	conditions	1911:1920	arg1	presence					1883:1890	the presence	1879:1890	the presence of complex chronic conditions	1879:1920	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	10	62	theme	conditions	1911:1920	arg1	specialty					1868:1876	surgical specialty	1859:1876	surgical specialty	1859:1876	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	10	62	theme	conditions	1911:1920	arg1	technique					1938:1946	anesthesia technique	1927:1946	anesthesia technique	1927:1946	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	3	63	theme	retrospective	580:592	arg1	study					594:598	a propensity score-matched retrospective study	553:598	a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	553:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	10	64	theme	complex	1895:1901	arg1	conditions					1911:1920	complex chronic conditions	1895:1920	complex chronic conditions	1895:1920	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	3	65	theme	Information	627:637	arg1	dataset					653:659	the Pediatric Health Information System (PHIS) dataset	606:659	the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	606:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	9	66	theme	NMB	1647:1649	arg1	agent					1660:1664	NMB reversal agent	1647:1664	NMB reversal agent	1647:1664	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	0	67	from	Complications	76:88	arg1	Children					93:100	Children	93:100	Children	93:100	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children.
35020682	3	68	theme	Pediatric	610:618	arg1	dataset					653:659	the Pediatric Health Information System (PHIS) dataset	606:659	the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020	606:692	METHOD We performed a propensity score-matched retrospective study using the Pediatric Health Information System (PHIS) dataset spanning the years 2016 and 2020.
35020682	7	69	dep	ratio	1395:1399	arg1	[OR					1401:1403	[OR	1401:1403	odds ratio [OR	1390:1403	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	9	70	theme	agent	1660:1664	arg1	Choice					1637:1642	CONCLUSIONS Choice	1625:1642	CONCLUSIONS Choice of NMB reversal agent	1625:1664	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	6	71	theme	33,819	1068:1073	arg1	children					1075:1082	33,819 children	1068:1082	33,819 children	1068:1082	RESULTS Our study included a study population of 33,819 children, of whom 23,312 (68.9%) received neostigmine and 10,507 (31.1%) received sugammadex.
35020682	6	72	dep	RESULTS	1019:1025	arg1	included					1037:1044	included	1037:1044	included a study population of 33,819 children, of whom 23,312 (68.9%) received neostigmine and 10,507 (31.1%) received sugammadex	1037:1166	RESULTS Our study included a study population of 33,819 children, of whom 23,312 (68.9%) received neostigmine and 10,507 (31.1%) received sugammadex.
35020682	7	73	theme	complications	1361:1373	arg1	incidence					1338:1346	the incidence	1334:1346	the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19)	1334:1461	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	7	73	theme	complications	1361:1373	arg1	evidence					1247:1254	no evidence	1244:1254	no evidence of a statistically significant association between the NMB reversal agent	1244:1328	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	4	74	theme	elective	739:746	arg1	procedures					780:789	elective, inpatient, noncardiac surgical procedures	739:789	elective, inpatient, noncardiac surgical procedures	739:789	We studied children <18 years who underwent elective, inpatient, noncardiac surgical procedures and received either neostigmine or sugammadex for reversal of NMB.
35020682	1	75	theme	complications	339:351	arg1	risk					297:300	the risk	293:300	the risk of composite postoperative pulmonary complications	293:351	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	5	76	theme	respiratory	998:1008	arg1	failure					1010:1016	respiratory failure	998:1016	respiratory failure	998:1016	Our primary outcome was major postoperative pulmonary complication, which we defined as the occurrence of either postoperative pneumonia or respiratory failure.
35020682	4	77	dep	children	706:713	arg1	years					719:723	<18 years	715:723	children <18 years who underwent elective, inpatient, noncardiac surgical procedures and received either neostigmine or sugammadex for reversal of NMB	706:855	We studied children <18 years who underwent elective, inpatient, noncardiac surgical procedures and received either neostigmine or sugammadex for reversal of NMB.
35020682	9	78	theme	postoperative	1715:1727	arg1	complications					1739:1751	major postoperative pulmonary complications	1709:1751	major postoperative pulmonary complications	1709:1751	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	7	79	theme	reversal	1315:1322	arg1	agent					1324:1328	the NMB reversal agent	1307:1328	the NMB reversal agent	1307:1328	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	9	80	theme	complications	1739:1751	arg1	incidence					1696:1704	the incidence	1692:1704	the incidence of major postoperative pulmonary complications	1692:1751	CONCLUSIONS Choice of NMB reversal agent does not appear to impact the incidence of major postoperative pulmonary complications.
35020682	2	81	theme	pulmonary	391:399	arg1	complications					401:413	pulmonary complications	391:413	pulmonary complications	391:413	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	1	82	theme	Recent	114:119	arg1	data					121:124	BACKGROUND Recent data	103:124	BACKGROUND Recent data in adult patients	103:142	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	10	83	theme	Further	1754:1760	arg1	research					1762:1769	Further research	1754:1769	Further research	1754:1769	Further research is needed to determine whether our results carry forth across subpopulations defined by surgical specialty, the presence of complex chronic conditions, and anesthesia technique.
35020682	8	84	theme	pulmonary	1482:1490	arg1	complications					1492:1504	pulmonary complications	1482:1504	pulmonary complications	1482:1504	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
35020682	7	85	theme	association	1287:1297	arg1	incidence					1338:1346	the incidence	1334:1346	the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19)	1334:1461	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	7	85	theme	association	1287:1297	arg1	evidence					1247:1254	no evidence	1244:1254	no evidence of a statistically significant association between the NMB reversal agent	1244:1328	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	0	86	theme	Postoperative	52:64	arg1	Complications					76:88	Major Postoperative Pulmonary Complications	46:88	Major Postoperative Pulmonary Complications in Children	46:100	Association of Sugammadex or Neostigmine With Major Postoperative Pulmonary Complications in Children.
35020682	8	87	theme	respiratory	1517:1527	arg1	failure					1529:1535	respiratory failure	1517:1535	respiratory failure	1517:1535	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
35020682	4	88	theme	NMB	853:855	arg1	reversal					841:848	reversal	841:848	reversal of NMB	841:855	We studied children <18 years who underwent elective, inpatient, noncardiac surgical procedures and received either neostigmine or sugammadex for reversal of NMB.
35020682	1	89	theme	postoperative	315:327	arg1	complications					339:351	composite postoperative pulmonary complications	305:351	composite postoperative pulmonary complications	305:351	BACKGROUND Recent data in adult patients indicate that the use of sugammadex compared to neostigmine for reversal of neuromuscular block (NMB) was associated with a significant reduction in the risk of composite postoperative pulmonary complications.
35020682	7	90	theme	P	1454:1454	arg1	.19					1458:1460	P = .19	1454:1460	P = .19	1454:1460	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	4	91	dep	elective	739:746	arg1	noncardiac					760:769	noncardiac	760:769	noncardiac	760:769	We studied children <18 years who underwent elective, inpatient, noncardiac surgical procedures and received either neostigmine or sugammadex for reversal of NMB.
35020682	4	91	dep	elective	739:746	arg1	inpatient					749:757	inpatient	749:757	inpatient	749:757	We studied children <18 years who underwent elective, inpatient, noncardiac surgical procedures and received either neostigmine or sugammadex for reversal of NMB.
35020682	7	92	dep	complications	1361:1373	arg1	0.90					1407:1410	0.90	1407:1410	0.90	1407:1410	After propensity score matching (10,361 matched from each group), we found no evidence of a statistically significant association between the NMB reversal agent and the incidence of pulmonary complications (3.1% vs 3.1%; odds ratio [OR], 0.90; 95% confidence interval [CI], 0.78-1.05; P = .19).
35020682	2	93	theme	reversal	462:469	arg1	role					450:453	the role	446:453	the role of NMB reversal in the risk of these complications	446:504	Despite the clinical significance of pulmonary complications in children, studies exploring the role of NMB reversal in the risk of these complications are currently unavailable.
35020682	8	94	theme	reversal	1609:1616	arg1	agent					1618:1622	NMB reversal agent	1605:1622	NMB reversal agent	1605:1622	The components of pulmonary complications, including respiratory failure and pneumonia, were not statistically associated with the choice of NMB reversal agent.
36911987	0	0	theme	citrus	86:91	arg1	fruit					93:97	citrus fruit	86:97	citrus fruit	86:97	The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit.
36911987	10	1	from	differences	1447:1457	arg1	composition					1468:1478	sugar composition	1462:1478	sugar composition of 'Youliang' and 'Gongchuan' fruit	1462:1514	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	5	2	theme	Synthase	482:489	arg1	CitSUS5					497:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	8	3	theme	sucrose	1061:1067	arg1	metabolism					1069:1078	sucrose metabolism	1061:1078	sucrose metabolism	1061:1078	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	5	4	theme	Youliang	717:724	arg1	fruit					727:731	'Youliang' fruit	716:731	'Youliang' fruit	716:731	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	5	5	from	levels	706:711	arg1	fruit					727:731	'Youliang' fruit	716:731	'Youliang' fruit	716:731	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	10	6	theme	hexose	1330:1335	arg1	proportion					1337:1346	the hexose proportion	1326:1346	the hexose proportion in citrus	1326:1356	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	10	7	theme	molecular	1413:1421	arg1	mechanism					1423:1431	the molecular mechanism	1409:1431	the molecular mechanism	1409:1431	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	5	8	theme	varieties	669:677	arg1	stages					649:654	different developmental stages	625:654	different developmental stages of these two varieties	625:677	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	6	9	theme	CitSWEET6	759:767	arg1	roles					738:742	The roles	734:742	The roles of CitSUS5 and CitSWEET6	734:767	The roles of CitSUS5 and CitSWEET6 were investigated by enzyme activity and transient assays.
36911987	6	10	theme	CitSUS5	747:753	arg1	roles					738:742	The roles	734:742	The roles of CitSUS5 and CitSWEET6	734:767	The roles of CitSUS5 and CitSWEET6 were investigated by enzyme activity and transient assays.
36911987	7	11	theme	fructose	915:922	arg1	transporter					924:934	a fructose transporter	913:934	a fructose transporter	913:934	CitSUS5 promoted the cleavage of sucrose to hexoses, and CitSWEET6 was identified as a fructose transporter.
36911987	7	11	theme	fructose	915:922	arg1	CitSWEET6					885:893	CitSWEET6	885:893	CitSWEET6	885:893	CitSUS5 promoted the cleavage of sucrose to hexoses, and CitSWEET6 was identified as a fructose transporter.
36911987	5	12	from	stages	649:654	arg1	analysis					613:620	transcriptome analysis	599:620	transcriptome analysis at different developmental stages of these two varieties	599:677	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	5	13	theme	transcriptome	599:611	arg1	analysis					613:620	transcriptome analysis	599:620	transcriptome analysis at different developmental stages of these two varieties	599:677	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	2	14	dep	accumulation	173:184	arg1	the					169:171	the	169:171	the	169:171	For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness.
36911987	5	15	theme	higher	688:693	arg1	levels					706:711	higher expression levels	688:711	higher expression levels in 'Youliang' fruit	688:731	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	8	16	theme	Further	937:943	arg1	investigation					945:957	Further investigation	937:957	Further investigation	937:957	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	4	17	theme	Gongchuan	382:390	arg1	fruits					371:376	mature fruits	364:376	mature fruits of 'Gongchuan'	364:391	, we found the difference in sweetness between mature fruits of 'Gongchuan' and its bud sport 'Youliang' is related to hexose contents.
36911987	3	18	dep	Blanco	309:314	arg1	citrus					283:288	citrus	283:288	citrus	283:288	In citrus (Citrus reticulata Blanco.)
36911987	8	19	theme	ZINC	1004:1007	arg1	CitZAT5					995:1001	CitZAT5	995:1001	the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6	970:1153	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	8	19	theme	ZINC	1004:1007	arg1	FINGER					1009:1014	ZINC FINGER	1004:1014	ZINC FINGER OF ARABIDOPSIS THALIANA	1004:1038	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	9	20	theme	CitZAT5	1168:1174	arg1	role					1160:1163	The role	1156:1163	The role of CitZAT5 in fruit sugar accumulation and hexose proportion	1156:1224	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	2	21	theme	attractiveness	264:277	arg1	development					237:247	the development	233:247	the development of quality and attractiveness	233:277	For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness.
36911987	8	22	dep	factor	988:993	arg1	CitZAT5					995:1001	CitZAT5	995:1001	the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6	970:1153	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	8	22	dep	factor	988:993	arg1	FINGER					1009:1014	ZINC FINGER	1004:1014	ZINC FINGER OF ARABIDOPSIS THALIANA	1004:1038	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	0	23	theme	transcription	4:16	arg1	CitZAT5					25:31	The transcription factor CitZAT5	0:31	The transcription factor CitZAT5	0:31	The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit.
36911987	4	24	theme	hexose	436:441	arg1	contents					443:450	hexose contents	436:450	hexose contents	436:450	, we found the difference in sweetness between mature fruits of 'Gongchuan' and its bud sport 'Youliang' is related to hexose contents.
36911987	5	25	theme	gene	566:569	arg1	CitSWEET6					571:579	a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6	509:579	a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6	509:579	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	5	26	theme	SWEET	511:515	arg1	CitSWEET6					571:579	a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6	509:579	a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6	509:579	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	10	27	from	proportion	1337:1346	arg1	citrus					1351:1356	citrus	1351:1356	citrus	1351:1356	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	5	28	theme	developmental	635:647	arg1	stages					649:654	different developmental stages	625:654	different developmental stages of these two varieties	625:677	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	5	29	theme	Exported	544:551	arg1	Transporter					553:563	Exported Transporter	544:563	Exported Transporter	544:563	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	8	30	theme	transcription	974:986	arg1	factor					988:993	the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA)	970:1039	the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6	970:1153	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	9	31	theme	fruit	1179:1183	arg1	accumulation					1191:1202	fruit sugar accumulation	1179:1202	fruit sugar accumulation	1179:1202	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	4	32	theme	mature	364:369	arg1	fruits					371:376	mature fruits	364:376	mature fruits of 'Gongchuan'	364:391	, we found the difference in sweetness between mature fruits of 'Gongchuan' and its bud sport 'Youliang' is related to hexose contents.
36911987	0	33	theme	factor	18:23	arg1	CitZAT5					25:31	The transcription factor CitZAT5	0:31	The transcription factor CitZAT5	0:31	The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit.
36911987	10	34	theme	fruit	1510:1514	arg1	composition					1468:1478	sugar composition	1462:1478	sugar composition of 'Youliang' and 'Gongchuan' fruit	1462:1514	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	8	35	theme	THALIANA	1031:1038	arg1	CitZAT5					995:1001	CitZAT5	995:1001	the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6	970:1153	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	8	35	theme	THALIANA	1031:1038	arg1	FINGER					1009:1014	ZINC FINGER	1004:1014	ZINC FINGER OF ARABIDOPSIS THALIANA	1004:1038	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	6	36	theme	transient	810:818	arg1	assays					820:825	transient assays	810:825	transient assays	810:825	The roles of CitSUS5 and CitSWEET6 were investigated by enzyme activity and transient assays.
36911987	0	37	theme	sugar	42:46	arg1	accumulation					48:59	sugar accumulation	42:59	sugar accumulation	42:59	The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit.
36911987	10	38	theme	Youliang	1484:1491	arg1	fruit					1510:1514	'Youliang' and 'Gongchuan' fruit	1483:1514	'Youliang' and 'Gongchuan' fruit	1483:1514	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	5	39	dep	CitSWEET6	571:579	arg1	Transporter					553:563	Exported Transporter	544:563	Exported Transporter	544:563	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	9	40	theme	hexose	1208:1213	arg1	proportion					1215:1224	hexose proportion	1208:1224	hexose proportion	1208:1224	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	4	41	from	difference	332:341	arg1	sweetness					346:354	sweetness	346:354	sweetness	346:354	, we found the difference in sweetness between mature fruits of 'Gongchuan' and its bud sport 'Youliang' is related to hexose contents.
36911987	5	42	theme	expression	695:704	arg1	levels					706:711	higher expression levels	688:711	higher expression levels in 'Youliang' fruit	688:731	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	9	43	theme	homologous	1246:1255	arg1	CitZAT5-overexpression					1267:1288	homologous transient CitZAT5-overexpression	1246:1288	homologous transient CitZAT5-overexpression	1246:1288	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	10	44	theme	sugar	1462:1466	arg1	composition					1468:1478	sugar composition	1462:1478	sugar composition of 'Youliang' and 'Gongchuan' fruit	1462:1514	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	3	45	theme	reticulata	298:307	arg1	Blanco					309:314	Citrus reticulata Blanco	291:314	Citrus reticulata Blanco.	291:315	In citrus (Citrus reticulata Blanco.)
36911987	6	46	theme	enzyme	790:795	arg1	activity					797:804	enzyme activity	790:804	enzyme activity	790:804	The roles of CitSUS5 and CitSWEET6 were investigated by enzyme activity and transient assays.
36911987	5	47	theme	gene	492:495	arg1	CitSUS5					497:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	2	48	theme	crucial	216:222	arg1	roles					224:228	crucial roles	216:228	crucial roles	216:228	For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness.
36911987	5	49	theme	CitSUS5	497:503	arg1	Expression					453:462	Expression	453:462	Expression of a SuS (Sucrose Synthase) gene CitSUS5	453:503	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	5	49	theme	CitSUS5	497:503	arg1	CitSWEET6					571:579	a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6	509:579	a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6	509:579	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	9	50	theme	sugar	1185:1189	arg1	accumulation					1191:1202	fruit sugar accumulation	1179:1202	fruit sugar accumulation	1179:1202	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	5	51	theme	different	625:633	arg1	stages					649:654	different developmental stages	625:654	different developmental stages of these two varieties	625:677	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	2	52	theme	sugars	204:209	arg1	accumulation					173:184	accumulation	173:184	accumulation	173:184	For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness.
36911987	2	52	theme	sugars	204:209	arg1	proportion					190:199	proportion	190:199	proportion	190:199	For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness.
36911987	10	53	theme	CitSWEET6	1383:1391	arg1	expression					1393:1402	CitSWEET6 expression	1383:1402	CitSWEET6 expression	1383:1402	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	1	54	theme	plant	126:130	arg1	processes					146:154	plant developmental processes	126:154	plant developmental processes	126:154	Sugars are fundamental to plant developmental processes.
36911987	7	55	theme	sucrose	861:867	arg1	cleavage					849:856	the cleavage	845:856	the cleavage of sucrose to hexoses	845:878	CitSUS5 promoted the cleavage of sucrose to hexoses, and CitSWEET6 was identified as a fructose transporter.
36911987	3	56	theme	Citrus	291:296	arg1	Blanco					309:314	Citrus reticulata Blanco	291:314	Citrus reticulata Blanco.	291:315	In citrus (Citrus reticulata Blanco.)
36911987	4	57	theme	sport	405:409	arg1	Youliang					412:419	its bud sport 'Youliang'	397:420	its bud sport 'Youliang'	397:420	, we found the difference in sweetness between mature fruits of 'Gongchuan' and its bud sport 'Youliang' is related to hexose contents.
36911987	2	58	theme	quality	252:258	arg1	development					237:247	the development	233:247	the development of quality and attractiveness	233:277	For fruits, the accumulation and proportion of sugars play crucial roles in the development of quality and attractiveness.
36911987	5	59	theme	SuS	469:471	arg1	CitSUS5					497:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
36911987	1	60	theme	developmental	132:144	arg1	processes					146:154	plant developmental processes	126:154	plant developmental processes	126:154	Sugars are fundamental to plant developmental processes.
36911987	8	61	theme	fructose	1084:1091	arg1	transportation					1093:1106	fructose transportation	1084:1106	fructose transportation	1084:1106	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	4	62	theme	bud	401:403	arg1	Youliang					412:419	its bud sport 'Youliang'	397:420	its bud sport 'Youliang'	397:420	, we found the difference in sweetness between mature fruits of 'Gongchuan' and its bud sport 'Youliang' is related to hexose contents.
36911987	10	63	theme	Gongchuan	1499:1507	arg1	fruit					1510:1514	'Youliang' and 'Gongchuan' fruit	1483:1514	'Youliang' and 'Gongchuan' fruit	1483:1514	CitZAT5 modulates the hexose proportion in citrus by mediating CitSUS5 and CitSWEET6 expression, and the molecular mechanism explained the differences in sugar composition of 'Youliang' and 'Gongchuan' fruit.
36911987	9	64	from	role	1160:1163	arg1	accumulation					1191:1202	fruit sugar accumulation	1179:1202	fruit sugar accumulation	1179:1202	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	9	64	from	role	1160:1163	arg1	proportion					1215:1224	hexose proportion	1208:1224	hexose proportion	1208:1224	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	0	65	theme	hexose	65:70	arg1	proportion					72:81	hexose proportion	65:81	hexose proportion	65:81	The transcription factor CitZAT5 modifies sugar accumulation and hexose proportion in citrus fruit.
36911987	9	66	theme	transient	1257:1265	arg1	CitZAT5-overexpression					1267:1288	homologous transient CitZAT5-overexpression	1246:1288	homologous transient CitZAT5-overexpression	1246:1288	The role of CitZAT5 in fruit sugar accumulation and hexose proportion was investigated by homologous transient CitZAT5-overexpression, -VIGS and -RNAi.
36911987	8	67	theme	ARABIDOPSIS	1019:1029	arg1	THALIANA					1031:1038	ARABIDOPSIS THALIANA	1019:1038	ARABIDOPSIS THALIANA	1019:1038	Further investigation identified the transcription factor CitZAT5 (ZINC FINGER OF ARABIDOPSIS THALIANA) that contributes to sucrose metabolism and fructose transportation by positively regulating CitSUS5 and CitSWEET6.
36911987	5	68	theme	Sucrose	474:480	arg1	CitSUS5					497:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	a SuS (Sucrose Synthase) gene CitSUS5	467:503	Expression of a SuS (Sucrose Synthase) gene CitSUS5 and a SWEET (Sugars Will Eventually be Exported Transporter) gene CitSWEET6, characterized by transcriptome analysis at different developmental stages of these two varieties revealed higher expression levels in 'Youliang' fruit.
37120014	0	0	theme	diabetic	77:84	arg1	mice					86:89	type 2 diabetic mice	70:89	type 2 diabetic mice	70:89	High-intensity interval training induces renal injury and fibrosis in type 2 diabetic mice.
37120014	4	1	theme	MATERIALS	425:433	arg1	mice					452:455	MATERIALS AND METHODS T2DM mice	425:455	MATERIALS AND METHODS T2DM mice	425:455	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	13	2	theme	renal	1574:1578	arg1	injury					1580:1585	renal injury	1574:1585	renal injury	1574:1585	SIGNIFICANCE This study concluded that HIIT induced renal injury and fibrosis, although it also improved glucose homeostasis in T2DM mice.
37120014	7	3	theme	protein	896:902	arg1	levels					904:909	the protein levels	892:909	the protein levels	892:909	Western blotting was performed to detect the protein levels.
37120014	13	4	from	homeostasis	1635:1645	arg1	mice					1655:1658	T2DM mice	1650:1658	T2DM mice	1650:1658	SIGNIFICANCE This study concluded that HIIT induced renal injury and fibrosis, although it also improved glucose homeostasis in T2DM mice.
37120014	1	5	theme	mellitus	239:246	arg1	mice					255:258	type 2 diabetes mellitus (T2DM) mice	223:258	type 2 diabetes mellitus (T2DM) mice	223:258	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	4	6	theme	T2DM	447:450	arg1	mice					452:455	MATERIALS AND METHODS T2DM mice	425:455	MATERIALS AND METHODS T2DM mice	425:455	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	2	7	theme	mice	307:310	arg1	kidneys					296:302	the kidneys	292:302	the kidneys of mice with T2DM	292:320	However, the effect of HIIT on the kidneys of mice with T2DM has not been examined.
37120014	9	8	theme	mice	1129:1132	arg1	tolerance					1064:1072	glucose tolerance	1056:1072	glucose tolerance	1056:1072	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	9	8	theme	mice	1129:1132	arg1	tolerance					1083:1091	insulin tolerance	1075:1091	insulin tolerance	1075:1091	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	9	8	theme	mice	1129:1132	arg1	deposition					1110:1119	renal lipid deposition	1098:1119	renal lipid deposition of T2DM mice	1098:1132	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	13	9	theme	T2DM	1650:1653	arg1	mice					1655:1658	T2DM mice	1650:1658	T2DM mice	1650:1658	SIGNIFICANCE This study concluded that HIIT induced renal injury and fibrosis, although it also improved glucose homeostasis in T2DM mice.
37120014	4	10	theme	METHODS	439:445	arg1	mice					452:455	MATERIALS AND METHODS T2DM mice	425:455	MATERIALS AND METHODS T2DM mice	425:455	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	6	11	theme	red	735:737	arg1	staining					739:746	Sirius red staining	728:746	Sirius red staining	728:746	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	14	12	with	patients	1695:1702	arg1	T2DM					1709:1712	T2DM	1709:1712	T2DM	1709:1712	The current study reminds us that patients with T2DM should be cautious when participating in HIIT.
37120014	9	13	theme	insulin	1075:1081	arg1	tolerance					1083:1091	insulin tolerance	1075:1091	insulin tolerance	1075:1091	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	4	14	theme	one-time	501:508	arg1	injection					551:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	0	15	from	fibrosis	58:65	arg1	mice					86:89	type 2 diabetic mice	70:89	type 2 diabetic mice	70:89	High-intensity interval training induces renal injury and fibrosis in type 2 diabetic mice.
37120014	8	16	theme	body	960:963	arg1	composition					965:975	the body composition	956:975	the body composition	956:975	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	6	17	theme	Sirius	728:733	arg1	staining					739:746	Sirius red staining	728:746	Sirius red staining	728:746	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	3	18	theme	T2DM	414:417	arg1	mice					419:422	T2DM mice	414:422	T2DM mice	414:422	This study aimed to investigate the impact of HIIT on the kidneys of T2DM mice.
37120014	4	19	theme	HIIT	610:613	arg1	8 weeks					599:605	8 weeks	599:605	8 weeks of HIIT	599:613	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	4	20	theme	T2DM	571:574	arg1	mice					576:579	T2DM mice	571:579	T2DM mice	571:579	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	6	21	used	used	805:808	arg2	staining					739:746	Sirius red staining	728:746	Sirius red staining	728:746	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	6	21	used	used	805:808	arg2	staining					791:798	Oil red O staining	781:798	Oil red O staining	781:798	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	6	21	used	used	805:808	arg2	staining					767:774	hematoxylin-eosin staining	749:774	hematoxylin-eosin staining	749:774	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	9	22	theme	renal	1098:1102	arg1	deposition					1110:1119	renal lipid deposition	1098:1119	renal lipid deposition of T2DM mice	1098:1132	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	8	23	dep	HIIT	925:928	arg1	ameliorated					944:954	ameliorated	944:954	HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice	925:1034	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	11	24	theme	Western	1245:1251	arg1	analysis					1258:1265	Western blot analysis	1245:1265	Western blot analysis	1245:1265	Western blot analysis showed that the PI3K/AKT/mTOR signaling pathway was activated after HIIT.
37120014	0	25	from	injury	47:52	arg1	mice					86:89	type 2 diabetic mice	70:89	type 2 diabetic mice	70:89	High-intensity interval training induces renal injury and fibrosis in type 2 diabetic mice.
37120014	11	26	theme	blot	1253:1256	arg1	analysis					1258:1265	Western blot analysis	1245:1265	Western blot analysis	1245:1265	Western blot analysis showed that the PI3K/AKT/mTOR signaling pathway was activated after HIIT.
37120014	7	27	theme	Western	851:857	arg1	blotting					859:866	Western blotting	851:866	Western blotting	851:866	Western blotting was performed to detect the protein levels.
37120014	4	28	theme	100 mg/kg	510:518	arg1	injection					551:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	10	29	theme	serum	1173:1177	arg1	creatinine					1179:1188	serum creatinine	1173:1188	serum creatinine	1173:1188	However, we found that HIIT increased serum creatinine and glycogen accumulation in the kidneys of T2DM mice.
37120014	11	30	theme	signaling	1297:1305	arg1	pathway					1307:1313	the PI3K/AKT/mTOR signaling pathway	1279:1313	the PI3K/AKT/mTOR signaling pathway	1279:1313	Western blot analysis showed that the PI3K/AKT/mTOR signaling pathway was activated after HIIT.
37120014	0	31	theme	interval	15:22	arg1	training					24:31	High-intensity interval training	0:31	High-intensity interval training	0:31	High-intensity interval training induces renal injury and fibrosis in type 2 diabetic mice.
37120014	6	32	theme	red	785:787	arg1	staining					791:798	Oil red O staining	781:798	Oil red O staining	781:798	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	8	33	theme	KEY	912:914	arg1	FINDINGS					916:923	KEY FINDINGS	912:923	KEY FINDINGS	912:923	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	2	34	theme	HIIT	284:287	arg1	effect					274:279	the effect	270:279	the effect of HIIT on the kidneys of mice with T2DM	270:320	However, the effect of HIIT on the kidneys of mice with T2DM has not been examined.
37120014	1	35	theme	fasting	175:181	arg1	glucose					189:195	fasting blood glucose	175:195	fasting blood glucose	175:195	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	0	36	theme	High-intensity	0:13	arg1	training					24:31	High-intensity interval training	0:31	High-intensity interval training	0:31	High-intensity interval training induces renal injury and fibrosis in type 2 diabetic mice.
37120014	4	37	theme	high-fat	477:484	arg1	HFD					492:494	HFD	492:494	HFD	492:494	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	4	37	theme	high-fat	477:484	arg1	diet					486:489	a high-fat diet	475:489	a high-fat diet (HFD)	475:495	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	8	38	theme	blood	986:990	arg1	glucose					992:998	fasting blood glucose	978:998	fasting blood glucose	978:998	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	8	39	theme	fasting	978:984	arg1	glucose					992:998	fasting blood glucose	978:998	fasting blood glucose	978:998	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	12	40	theme	fibrosis-related	1359:1374	arg1	proteins					1376:1383	fibrosis-related proteins	1359:1383	fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA)	1359:1419	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	1	41	theme	blood	183:187	arg1	glucose					189:195	fasting blood glucose	175:195	fasting blood glucose	175:195	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	12	42	theme	MMP13	1477:1481	arg1	expression					1442:1451	the expression	1438:1451	the expression of klotho (sklotho) and MMP13	1438:1481	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	5	43	theme	Renal	616:620	arg1	function					622:629	Renal function	616:629	Renal function	616:629	Renal function and glycogen deposition were observed by serum creatinine levels and PAS staining, respectively.
37120014	9	44	theme	T2DM	1124:1127	arg1	mice					1129:1132	T2DM mice	1124:1132	T2DM mice	1124:1132	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	1	45	theme	AIMS	92:95	arg1	studies					106:112	AIMS Previous studies	92:112	AIMS Previous studies	92:112	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	6	46	theme	O	789:789	arg1	staining					791:798	Oil red O staining	781:798	Oil red O staining	781:798	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	1	47	theme	Previous	97:104	arg1	studies					106:112	AIMS Previous studies	92:112	AIMS Previous studies	92:112	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	12	48	theme	mice	1516:1519	arg1	kidneys					1500:1506	the kidneys	1496:1506	the kidneys of HIIT mice	1496:1519	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	5	49	theme	PAS	700:702	arg1	staining					704:711	PAS staining	700:711	PAS staining	700:711	Renal function and glycogen deposition were observed by serum creatinine levels and PAS staining, respectively.
37120014	14	50	theme	current	1665:1671	arg1	study					1673:1677	The current study	1661:1677	The current study	1661:1677	The current study reminds us that patients with T2DM should be cautious when participating in HIIT.
37120014	1	51	theme	insulin	201:207	arg1	resistance					209:218	insulin resistance	201:218	insulin resistance	201:218	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	10	52	theme	glycogen	1194:1201	arg1	accumulation					1203:1214	glycogen accumulation	1194:1214	glycogen accumulation	1194:1214	However, we found that HIIT increased serum creatinine and glycogen accumulation in the kidneys of T2DM mice.
37120014	3	53	theme	HIIT	391:394	arg1	impact					381:386	the impact	377:386	the impact of HIIT on the kidneys of T2DM mice	377:422	This study aimed to investigate the impact of HIIT on the kidneys of T2DM mice.
37120014	0	54	theme	renal	41:45	arg1	injury					47:52	renal injury	41:52	renal injury	41:52	High-intensity interval training induces renal injury and fibrosis in type 2 diabetic mice.
37120014	12	55	theme	proteins	1376:1383	arg1	expression					1345:1354	The expression	1341:1354	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA)	1341:1419	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	5	56	theme	glycogen	635:642	arg1	deposition					644:653	glycogen deposition	635:653	glycogen deposition	635:653	Renal function and glycogen deposition were observed by serum creatinine levels and PAS staining, respectively.
37120014	6	57	theme	Oil	781:783	arg1	staining					791:798	Oil red O staining	781:798	Oil red O staining	781:798	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	10	58	theme	mice	1239:1242	arg1	kidneys					1223:1229	the kidneys	1219:1229	the kidneys of T2DM mice	1219:1242	However, we found that HIIT increased serum creatinine and glycogen accumulation in the kidneys of T2DM mice.
37120014	13	59	dep	concluded	1546:1554	arg1	SIGNIFICANCE					1522:1533	SIGNIFICANCE	1522:1533	SIGNIFICANCE	1522:1533	SIGNIFICANCE This study concluded that HIIT induced renal injury and fibrosis, although it also improved glucose homeostasis in T2DM mice.
37120014	12	60	dep	proteins	1376:1383	arg1	collagen-III					1400:1411	collagen-III	1400:1411	collagen-III	1400:1411	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	12	60	dep	proteins	1376:1383	arg1	CTGF					1394:1397	CTGF	1394:1397	CTGF	1394:1397	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	12	60	dep	proteins	1376:1383	arg1	TGF-β1					1386:1391	TGF-β1	1386:1391	TGF-β1	1386:1391	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	12	60	dep	proteins	1376:1383	arg1	α-SMA					1414:1418	α-SMA	1414:1418	α-SMA	1414:1418	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	11	61	theme	PI3K/AKT/mTOR	1283:1295	arg1	pathway					1307:1313	the PI3K/AKT/mTOR signaling pathway	1279:1313	the PI3K/AKT/mTOR signaling pathway	1279:1313	Western blot analysis showed that the PI3K/AKT/mTOR signaling pathway was activated after HIIT.
37120014	10	62	theme	T2DM	1234:1237	arg1	mice					1239:1242	T2DM mice	1234:1242	T2DM mice	1234:1242	However, we found that HIIT increased serum creatinine and glycogen accumulation in the kidneys of T2DM mice.
37120014	3	63	theme	mice	419:422	arg1	kidneys					403:409	the kidneys	399:409	the kidneys of T2DM mice	399:422	This study aimed to investigate the impact of HIIT on the kidneys of T2DM mice.
37120014	8	64	theme	serum	1005:1009	arg1	insulin					1011:1017	serum insulin	1005:1017	serum insulin	1005:1017	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	8	65	theme	mice	1031:1034	arg1	composition					965:975	the body composition	956:975	the body composition	956:975	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	8	65	theme	mice	1031:1034	arg1	insulin					1011:1017	serum insulin	1005:1017	serum insulin	1005:1017	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	8	65	theme	mice	1031:1034	arg1	glucose					992:998	fasting blood glucose	978:998	fasting blood glucose	978:998	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	2	66	with	mice	307:310	arg1	T2DM					317:320	T2DM	317:320	T2DM	317:320	However, the effect of HIIT on the kidneys of mice with T2DM has not been examined.
37120014	9	67	theme	lipid	1104:1108	arg1	deposition					1110:1119	renal lipid deposition	1098:1119	renal lipid deposition of T2DM mice	1098:1132	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	4	68	theme	streptozotocin	520:533	arg1	injection					551:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	8	69	theme	T2DM	1026:1029	arg1	mice					1031:1034	the T2DM mice	1022:1034	the T2DM mice	1022:1034	KEY FINDINGS HIIT significantly ameliorated the body composition, fasting blood glucose, and serum insulin of the T2DM mice.
37120014	6	70	theme	hematoxylin-eosin	749:765	arg1	staining					767:774	hematoxylin-eosin staining	749:774	hematoxylin-eosin staining	749:774	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	9	71	theme	glucose	1056:1062	arg1	tolerance					1064:1072	glucose tolerance	1056:1072	glucose tolerance	1056:1072	HIIT also improved glucose tolerance, insulin tolerance, and renal lipid deposition of T2DM mice.
37120014	1	72	theme	high-intensity	126:139	arg1	training					150:157	high-intensity interval training	126:157	high-intensity interval training (HIIT)	126:164	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	1	72	theme	high-intensity	126:139	arg1	HIIT					160:163	HIIT	160:163	HIIT	160:163	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	1	73	theme	type	223:226	arg1	T2DM					249:252	T2DM	249:252	T2DM	249:252	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	1	73	theme	type	223:226	arg1	mellitus					239:246	type 2 diabetes mellitus	223:246	type 2 diabetes mellitus (T2DM) mice	223:258	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	5	74	theme	serum	672:676	arg1	levels					689:694	serum creatinine levels	672:694	serum creatinine levels	672:694	Renal function and glycogen deposition were observed by serum creatinine levels and PAS staining, respectively.
37120014	13	75	theme	glucose	1627:1633	arg1	homeostasis					1635:1645	glucose homeostasis	1627:1645	glucose homeostasis in T2DM mice	1627:1658	SIGNIFICANCE This study concluded that HIIT induced renal injury and fibrosis, although it also improved glucose homeostasis in T2DM mice.
37120014	1	76	theme	interval	141:148	arg1	training					150:157	high-intensity interval training	126:157	high-intensity interval training (HIIT)	126:164	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	1	76	theme	interval	141:148	arg1	HIIT					160:163	HIIT	160:163	HIIT	160:163	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	12	77	theme	HIIT	1511:1514	arg1	mice					1516:1519	HIIT mice	1511:1519	HIIT mice	1511:1519	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	12	78	theme	klotho	1456:1461	arg1	expression					1442:1451	the expression	1438:1451	the expression of klotho (sklotho) and MMP13	1438:1481	The expression of fibrosis-related proteins (TGF-β1, CTGF, collagen-III, α-SMA) increased, while the expression of klotho (sklotho) and MMP13 decreased in the kidneys of HIIT mice.
37120014	5	79	theme	creatinine	678:687	arg1	levels					689:694	serum creatinine levels	672:694	serum creatinine levels	672:694	Renal function and glycogen deposition were observed by serum creatinine levels and PAS staining, respectively.
37120014	6	80	theme	lipid	833:837	arg1	deposition					839:848	lipid deposition	833:848	lipid deposition	833:848	Sirius red staining, hematoxylin-eosin staining, and Oil red O staining were used to detect fibrosis and lipid deposition.
37120014	4	81	theme	intraperitoneal	535:549	arg1	injection					551:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	one-time 100 mg/kg streptozotocin intraperitoneal injection	501:559	MATERIALS AND METHODS T2DM mice were induced with a high-fat diet (HFD) and one-time 100 mg/kg streptozotocin intraperitoneal injection, and then T2DM mice were treated with 8 weeks of HIIT.
37120014	3	82	from	impact	381:386	arg1	kidneys					403:409	the kidneys	399:409	the kidneys of T2DM mice	399:422	This study aimed to investigate the impact of HIIT on the kidneys of T2DM mice.
37120014	2	83	from	effect	274:279	arg1	kidneys					296:302	the kidneys	292:302	the kidneys of mice with T2DM	292:320	However, the effect of HIIT on the kidneys of mice with T2DM has not been examined.
37120014	1	84	theme	diabetes	230:237	arg1	T2DM					249:252	T2DM	249:252	T2DM	249:252	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
37120014	1	84	theme	diabetes	230:237	arg1	mellitus					239:246	type 2 diabetes mellitus	223:246	type 2 diabetes mellitus (T2DM) mice	223:258	AIMS Previous studies showed that high-intensity interval training (HIIT) improved fasting blood glucose and insulin resistance in type 2 diabetes mellitus (T2DM) mice.
35751882	4	0	theme	control	532:538	arg1	mice					560:563	control (Foxp3YFP-Cre , WT) mice	532:563	mice	560:563	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	7	1	theme	WT	1388:1389	arg1	mice					1391:1394	WT mice	1388:1394	WT mice	1388:1394	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	4	2	theme	Foxp3YFP-Cre	541:552	arg1	mice					560:563	control (Foxp3YFP-Cre , WT) mice	532:563	mice	560:563	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	6	3	theme	DSS-induced	1195:1205	arg1	colitis					1207:1213	DSS-induced colitis	1195:1213	DSS-induced colitis in WT and KO mice	1195:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	6	4	from	severity	1183:1190	arg1	mice					1228:1231	WT and KO mice	1218:1231	WT and KO mice	1218:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	2	5	theme	immune	265:270	arg1	cells					272:276	immune cells	265:276	immune cells such as macrophages and T cells	265:308	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	2	5	theme	immune	265:270	arg1	macrophages					286:296	macrophages	286:296	macrophages	286:296	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	2	5	theme	immune	265:270	arg1	cells					304:308	T cells	302:308	T cells	302:308	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	6	6	theme	microbiota	1078:1087	arg1	depletion					1061:1069	depletion	1061:1069	depletion of gut microbiota by antibiotics	1061:1102	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	2	7	theme	T	302:302	arg1	cells					304:308	T cells	302:308	T cells	302:308	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	1	8	theme	effective	191:199	arg1	target					206:211	an effective drug target	188:211	an effective drug target in the cardiovascular system	188:240	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	4	9	theme	cells	853:857	arg1	infiltration					794:805	infiltration	794:805	infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria	794:881	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	7	10	theme	inflammatory	1340:1351	arg1	infiltration					1353:1364	alleviated inflammatory infiltration	1329:1364	alleviated inflammatory infiltration compared to that from WT mice	1329:1394	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	4	11	theme	dextran	570:576	arg1	DSS					595:597	dextran sodium sulphate (DSS)	570:598	dextran sodium sulphate (DSS) to induce colitis	570:616	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	5	12	theme	gut	987:989	arg1	microbiota					991:1000	gut microbiota	987:1000	gut microbiota	987:1000	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	4	13	theme	colon	862:866	arg1	propria					875:881	colon lamina propria	862:881	colon lamina propria	862:881	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	4	14	theme	sulphate	585:592	arg1	DSS					595:597	dextran sodium sulphate (DSS)	570:598	dextran sodium sulphate (DSS) to induce colitis	570:616	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	6	15	theme	deficiency	1148:1157	arg1	effects					1129:1135	the protective effects	1114:1135	the protective effects of Treg MR deficiency	1114:1157	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	4	16	dep	mice	560:563	arg1	WT					556:557	control (Foxp3YFP-Cre , WT) mice	532:563	WT	556:557	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	4	17	theme	γ+	848:849	arg1	cells					853:857	interferon γ+ T cells	837:857	interferon γ+ T cells	837:857	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	6	18	theme	Treg	1140:1143	arg1	deficiency					1148:1157	Treg MR deficiency	1140:1157	Treg MR deficiency	1140:1157	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	2	19	theme	MR	259:260	arg1	function					247:254	The function	243:254	The function of MR in immune cells such as macrophages and T cells	243:308	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	7	20	theme	KO	1273:1274	arg1	mice					1276:1279	KO mice	1273:1279	KO mice	1273:1279	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	1	21	from	receptor	175:182	arg1	system					235:240	the cardiovascular system	216:240	the cardiovascular system	216:240	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	0	22	from	deficiency	27:36	arg1	cells					46:50	Treg cells	41:50	Treg cells	41:50	Mineralocorticoid receptor deficiency in Treg cells ameliorates DSS-induced colitis in a gut microbiota-dependent manner.
35751882	3	23	from	function	390:397	arg1	process					417:423	the process	413:423	the process of inflammatory bowel disease (IBD)	413:459	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	4	24	dep	alleviated	682:691	arg1	accompanied					720:730	accompanied	720:730	accompanied by reduced production of inflammatory cytokines	720:778	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	1	25	theme	Mineralocorticoid	122:138	arg1	MR					150:151	MR	150:151	MR	150:151	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	1	25	theme	Mineralocorticoid	122:138	arg1	receptor					140:147	Mineralocorticoid receptor	122:147	Mineralocorticoid receptor (MR)	122:152	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	1	25	theme	Mineralocorticoid	122:138	arg1	receptor					175:182	a classic nuclear receptor	157:182	a classic nuclear receptor	157:182	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	0	26	theme	microbiota-dependent	93:112	arg1	manner					114:119	a gut microbiota-dependent manner	87:119	a gut microbiota-dependent manner	87:119	Mineralocorticoid receptor deficiency in Treg cells ameliorates DSS-induced colitis in a gut microbiota-dependent manner.
35751882	4	27	dep	mice	523:526	arg1	KO					519:520	Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice	473:526	KO	519:520	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	8	28	theme	DSS-induced	1468:1478	arg1	colitis					1480:1486	DSS-induced colitis	1468:1486	DSS-induced colitis	1468:1486	Hence, our study demonstrates that Treg MR deficiency protects against DSS-induced colitis by attenuation of colonic inflammatory infiltration.
35751882	4	29	theme	MR-deficient	701:712	arg1	mice					714:717	Treg MR-deficient mice	696:717	Treg MR-deficient mice	696:717	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	5	30	theme	Treg	1024:1027	arg1	mice					1042:1045	Treg MR-deficient mice	1024:1045	Treg MR-deficient mice	1024:1045	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	4	31	theme	colitis	661:667	arg1	severity					637:644	the severity	633:644	the severity of DSS-induced colitis	633:667	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	3	32	theme	Treg	402:405	arg1	MR					407:408	Treg MR	402:408	Treg MR	402:408	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	0	33	theme	Mineralocorticoid	0:16	arg1	deficiency					27:36	Mineralocorticoid receptor deficiency	0:36	Mineralocorticoid receptor deficiency in Treg cells	0:50	Mineralocorticoid receptor deficiency in Treg cells ameliorates DSS-induced colitis in a gut microbiota-dependent manner.
35751882	9	34	theme	Gut	1541:1543	arg1	microbiota					1545:1554	Gut microbiota	1541:1554	Gut microbiota	1541:1554	Gut microbiota is both sufficient and necessary for Treg MR deficiency to exert the beneficial effects.
35751882	7	35	theme	microbiota	1241:1250	arg1	transplantation					1252:1266	Faecal microbiota transplantation	1234:1266	Faecal microbiota transplantation from KO mice	1234:1279	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	3	36	theme	inflammatory	428:439	arg1	IBD					456:458	IBD	456:458	IBD	456:458	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	3	36	theme	inflammatory	428:439	arg1	disease					447:453	inflammatory bowel disease	428:453	inflammatory bowel disease (IBD)	428:459	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	5	37	theme	Faecal	884:889	arg1	microbiota					891:900	Faecal microbiota	884:900	Faecal microbiota of mice with colitis	884:921	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	8	38	theme	inflammatory	1514:1525	arg1	infiltration					1527:1538	colonic inflammatory infiltration	1506:1538	colonic inflammatory infiltration	1506:1538	Hence, our study demonstrates that Treg MR deficiency protects against DSS-induced colitis by attenuation of colonic inflammatory infiltration.
35751882	0	39	theme	Treg	41:44	arg1	cells					46:50	Treg cells	41:50	Treg cells	41:50	Mineralocorticoid receptor deficiency in Treg cells ameliorates DSS-induced colitis in a gut microbiota-dependent manner.
35751882	5	40	theme	rRNA	943:946	arg1	sequencing					953:962	16S rRNA gene sequencing	939:962	16S rRNA gene sequencing	939:962	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	4	41	theme	MR-deficient	478:489	arg1	mice					523:526	Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice	473:526	mice	523:526	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	4	42	theme	inflammatory	757:768	arg1	cytokines					770:778	inflammatory cytokines	757:778	inflammatory cytokines	757:778	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	9	43	theme	beneficial	1625:1634	arg1	effects					1636:1642	the beneficial effects	1621:1642	the beneficial effects	1621:1642	Gut microbiota is both sufficient and necessary for Treg MR deficiency to exert the beneficial effects.
35751882	4	44	theme	MRflox/flox	492:502	arg1	mice					523:526	Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice	473:526	mice	523:526	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	3	45	theme	disease	447:453	arg1	process					417:423	the process	413:423	the process of inflammatory bowel disease (IBD)	413:459	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	1	46	theme	cardiovascular	220:233	arg1	system					235:240	the cardiovascular system	216:240	the cardiovascular system	216:240	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	9	47	theme	Treg	1593:1596	arg1	deficiency					1601:1610	Treg MR deficiency to exert the beneficial effects	1593:1642	Treg MR deficiency to exert the beneficial effects	1593:1642	Gut microbiota is both sufficient and necessary for Treg MR deficiency to exert the beneficial effects.
35751882	6	48	theme	WT	1218:1219	arg1	mice					1228:1231	WT and KO mice	1218:1231	WT and KO mice	1218:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	8	49	theme	MR	1437:1438	arg1	deficiency					1440:1449	Treg MR deficiency	1432:1449	Treg MR deficiency	1432:1449	Hence, our study demonstrates that Treg MR deficiency protects against DSS-induced colitis by attenuation of colonic inflammatory infiltration.
35751882	6	50	theme	colitis	1207:1213	arg1	severity					1183:1190	similar severity	1175:1190	similar severity of DSS-induced colitis in WT and KO mice	1175:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	4	51	theme	monocytes	810:818	arg1	infiltration					794:805	infiltration	794:805	infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria	794:881	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	4	52	theme	cytokines	770:778	arg1	production					743:752	reduced production	735:752	reduced production of inflammatory cytokines	735:778	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	6	53	theme	similar	1175:1181	arg1	severity					1183:1190	similar severity	1175:1190	similar severity of DSS-induced colitis in WT and KO mice	1175:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	6	54	theme	gut	1074:1076	arg1	microbiota					1078:1087	gut microbiota	1074:1087	gut microbiota	1074:1087	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	1	55	theme	drug	201:204	arg1	target					206:211	an effective drug target	188:211	an effective drug target in the cardiovascular system	188:240	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	4	56	theme	T	851:851	arg1	cells					853:857	interferon γ+ T cells	837:857	interferon γ+ T cells	837:857	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	1	57	theme	nuclear	167:173	arg1	receptor					175:182	a classic nuclear receptor	157:182	a classic nuclear receptor	157:182	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	1	57	theme	nuclear	167:173	arg1	receptor					140:147	Mineralocorticoid receptor	122:147	Mineralocorticoid receptor (MR)	122:152	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	5	58	theme	microbiota	991:1000	arg1	composition					972:982	the composition	968:982	the composition of gut microbiota	968:1000	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	7	59	theme	alleviated	1329:1338	arg1	infiltration					1353:1364	alleviated inflammatory infiltration	1329:1364	alleviated inflammatory infiltration compared to that from WT mice	1329:1394	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	4	60	theme	sodium	578:583	arg1	DSS					595:597	dextran sodium sulphate (DSS)	570:598	dextran sodium sulphate (DSS) to induce colitis	570:616	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	5	61	with	microbiota	891:900	arg1	colitis					915:921	colitis	915:921	colitis	915:921	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	4	62	theme	neutrophils	821:831	arg1	infiltration					794:805	infiltration	794:805	infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria	794:881	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	6	63	theme	MR	1145:1146	arg1	deficiency					1148:1157	Treg MR deficiency	1140:1157	Treg MR deficiency	1140:1157	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	6	64	theme	KO	1225:1226	arg1	mice					1228:1231	WT and KO mice	1218:1231	WT and KO mice	1218:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	1	65	from	target	206:211	arg1	system					235:240	the cardiovascular system	216:240	the cardiovascular system	216:240	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	4	66	theme	interferon	837:846	arg1	cells					853:857	interferon γ+ T cells	837:857	interferon γ+ T cells	837:857	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	8	67	theme	Treg	1432:1435	arg1	deficiency					1440:1449	Treg MR deficiency	1432:1449	Treg MR deficiency	1432:1449	Hence, our study demonstrates that Treg MR deficiency protects against DSS-induced colitis by attenuation of colonic inflammatory infiltration.
35751882	7	68	theme	DSS-induced	1292:1302	arg1	colitis					1304:1310	DSS-induced colitis	1292:1310	DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice	1292:1394	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	6	69	theme	protective	1118:1127	arg1	effects					1129:1135	the protective effects	1114:1135	the protective effects of Treg MR deficiency	1114:1157	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	6	70	from	colitis	1207:1213	arg1	mice					1228:1231	WT and KO mice	1218:1231	WT and KO mice	1218:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	0	71	theme	gut	89:91	arg1	manner					114:119	a gut microbiota-dependent manner	87:119	a gut microbiota-dependent manner	87:119	Mineralocorticoid receptor deficiency in Treg cells ameliorates DSS-induced colitis in a gut microbiota-dependent manner.
35751882	1	72	theme	classic	159:165	arg1	receptor					175:182	a classic nuclear receptor	157:182	a classic nuclear receptor	157:182	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	1	72	theme	classic	159:165	arg1	receptor					140:147	Mineralocorticoid receptor	122:147	Mineralocorticoid receptor (MR)	122:152	Mineralocorticoid receptor (MR) is a classic nuclear receptor and an effective drug target in the cardiovascular system.
35751882	4	73	theme	Treg	473:476	arg1	mice					523:526	Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice	473:526	mice	523:526	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	4	74	from	infiltration	794:805	arg1	propria					875:881	colon lamina propria	862:881	colon lamina propria	862:881	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	4	75	theme	lamina	868:873	arg1	propria					875:881	colon lamina propria	862:881	colon lamina propria	862:881	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	5	76	theme	mice	905:908	arg1	microbiota					891:900	Faecal microbiota	884:900	Faecal microbiota of mice with colitis	884:921	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	7	77	from	mice	1276:1279	arg1	transplantation					1252:1266	Faecal microbiota transplantation	1234:1266	Faecal microbiota transplantation from KO mice	1234:1279	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	4	78	theme	Treg	696:699	arg1	mice					714:717	Treg MR-deficient mice	696:717	Treg MR-deficient mice	696:717	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	0	79	theme	receptor	18:25	arg1	deficiency					27:36	Mineralocorticoid receptor deficiency	0:36	Mineralocorticoid receptor deficiency in Treg cells	0:50	Mineralocorticoid receptor deficiency in Treg cells ameliorates DSS-induced colitis in a gut microbiota-dependent manner.
35751882	5	80	theme	MR-deficient	1029:1040	arg1	mice					1042:1045	Treg MR-deficient mice	1024:1045	Treg MR-deficient mice	1024:1045	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	7	81	theme	Faecal	1234:1239	arg1	transplantation					1252:1266	Faecal microbiota transplantation	1234:1266	Faecal microbiota transplantation from KO mice	1234:1279	Faecal microbiota transplantation from KO mice attenuated DSS-induced colitis characterized by alleviated inflammatory infiltration compared to that from WT mice.
35751882	8	82	theme	colonic	1506:1512	arg1	infiltration					1527:1538	colonic inflammatory infiltration	1506:1538	colonic inflammatory infiltration	1506:1538	Hence, our study demonstrates that Treg MR deficiency protects against DSS-induced colitis by attenuation of colonic inflammatory infiltration.
35751882	4	83	theme	DSS-induced	649:659	arg1	colitis					661:667	DSS-induced colitis	649:667	DSS-induced colitis	649:667	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	3	84	theme	MR	407:408	arg1	function					390:397	the function	386:397	the function of Treg MR in the process of inflammatory bowel disease (IBD)	386:459	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	9	85	theme	MR	1598:1599	arg1	deficiency					1601:1610	Treg MR deficiency to exert the beneficial effects	1593:1642	Treg MR deficiency to exert the beneficial effects	1593:1642	Gut microbiota is both sufficient and necessary for Treg MR deficiency to exert the beneficial effects.
35751882	2	86	from	function	247:254	arg1	cells					272:276	immune cells	265:276	immune cells such as macrophages and T cells	265:308	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	2	86	from	function	247:254	arg1	macrophages					286:296	macrophages	286:296	macrophages	286:296	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	2	86	from	function	247:254	arg1	cells					304:308	T cells	302:308	T cells	302:308	The function of MR in immune cells such as macrophages and T cells has been increasingly appreciated.
35751882	3	87	theme	study	361:365	arg1	aim					349:351	The aim	345:351	The aim of this study	345:365	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	8	88	theme	infiltration	1527:1538	arg1	attenuation					1491:1501	attenuation	1491:1501	attenuation of colonic inflammatory infiltration	1491:1538	Hence, our study demonstrates that Treg MR deficiency protects against DSS-induced colitis by attenuation of colonic inflammatory infiltration.
35751882	3	89	theme	bowel	441:445	arg1	IBD					456:458	IBD	456:458	IBD	456:458	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	3	89	theme	bowel	441:445	arg1	disease					447:453	inflammatory bowel disease	428:453	inflammatory bowel disease (IBD)	428:459	The aim of this study was to investigate the function of Treg MR in the process of inflammatory bowel disease (IBD).
35751882	5	90	theme	16S	939:941	arg1	sequencing					953:962	16S rRNA gene sequencing	939:962	16S rRNA gene sequencing	939:962	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	4	91	theme	reduced	735:741	arg1	production					743:752	reduced production	735:752	reduced production of inflammatory cytokines	735:778	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	0	92	theme	DSS-induced	64:74	arg1	colitis					76:82	DSS-induced colitis	64:82	DSS-induced colitis	64:82	Mineralocorticoid receptor deficiency in Treg cells ameliorates DSS-induced colitis in a gut microbiota-dependent manner.
35751882	5	93	theme	gene	948:951	arg1	sequencing					953:962	16S rRNA gene sequencing	939:962	16S rRNA gene sequencing	939:962	Faecal microbiota of mice with colitis was analysed by 16S rRNA gene sequencing and the composition of gut microbiota was vastly changed in Treg MR-deficient mice.
35751882	4	94	theme	Foxp3YFP-Cre	504:515	arg1	mice					523:526	Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice	473:526	mice	523:526	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35751882	6	95	from	mice	1228:1231	arg1	severity					1183:1190	similar severity	1175:1190	similar severity of DSS-induced colitis in WT and KO mice	1175:1231	Furthermore, depletion of gut microbiota by antibiotics abolished the protective effects of Treg MR deficiency and resulted in similar severity of DSS-induced colitis in WT and KO mice.
35751882	4	96	dep	DSS	595:597	arg1	induce					603:608	induce	603:608	to induce colitis	600:616	We treated Treg MR-deficient (MRflox/flox Foxp3YFP-Cre , KO) mice and control (Foxp3YFP-Cre , WT) mice with dextran sodium sulphate (DSS) to induce colitis and found that the severity of DSS-induced colitis was markedly alleviated in Treg MR-deficient mice, accompanied by reduced production of inflammatory cytokines, and relieved infiltration of monocytes, neutrophils and interferon γ+ T cells in colon lamina propria.
35690592	1	0	theme	glycogen	145:152	arg1	metabolism					154:163	glycogen metabolism	145:163	glycogen metabolism	145:163	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	6	1	theme	increased	847:855	arg1	activity					879:886	increased basal/unstimulated GS activity	847:886	increased basal/unstimulated GS activity	847:886	Structure-guided mutagenesis perturbing interactions with phosphorylated tails led to increased basal/unstimulated GS activity.
35690592	6	2	theme	phosphorylated	819:832	arg1	tails					834:838	phosphorylated tails	819:838	phosphorylated tails	819:838	Structure-guided mutagenesis perturbing interactions with phosphorylated tails led to increased basal/unstimulated GS activity.
35690592	7	3	theme	multivalent	905:915	arg1	phosphorylation					917:931	multivalent phosphorylation	905:931	multivalent phosphorylation	905:931	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	1	4	from	defects	134:140	arg1	structure					169:177	structure	169:177	structure	169:177	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	1	4	from	defects	134:140	arg1	metabolism					154:163	glycogen metabolism	145:163	glycogen metabolism	145:163	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	7	5	from	region	1004:1009	arg1	interactions					968:979	interactions	968:979	interactions from a dynamic "spike" region	968:1009	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	5	6	theme	composite	732:740	arg1	"					758:758	a composite "arginine cradle"	730:758	a composite "arginine cradle"	730:758	This keeps GS in an inactive conformation mediated by phospho-Ser641 interactions with a composite "arginine cradle".
35690592	7	7	theme	"	1002:1002	arg1	region					1004:1009	a dynamic "spike" region	986:1009	a dynamic "spike" region	986:1009	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	4	8	from	protomers	552:560	arg1	N-					523:524	Phosphorylated N-	508:524	Phosphorylated N-	508:524	Phosphorylated N- and C-termini from two GS protomers converge near the G6P-binding pocket and buttress against GS regulatory helices.
35690592	7	9	theme	dynamic	988:994	arg1	"					1002:1002	a dynamic "spike"	986:1002	a dynamic "spike" region	986:1009	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	3	10	theme	phosphorylated	419:432	arg1	GS					440:441	phosphorylated human GS	419:441	phosphorylated human GS	419:441	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	7	11	theme	spike	997:1001	arg1	"					1002:1002	a dynamic "spike"	986:1002	a dynamic "spike" region	986:1009	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	8	12	theme	glycogen	1112:1119	arg1	regulation					1131:1140	glycogen synthesis regulation	1112:1140	glycogen synthesis regulation	1112:1140	This work therefore provides insights into glycogen synthesis regulation and facilitates studies of glycogen-related diseases.
35690592	3	13	theme	cryo-EM	398:404	arg1	structure					406:414	the 2.6 Å resolution cryo-EM structure	377:414	the 2.6 Å resolution cryo-EM structure of phosphorylated human GS	377:441	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	1	14	theme	major	93:97	arg1	Glycogen					77:84	Glycogen	77:84	Glycogen	77:84	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	1	14	theme	major	93:97	arg1	reserve					107:113	the major glucose reserve	89:113	the major glucose reserve in eukaryotes	89:127	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	0	15	theme	synthase	22:29	arg1	inactivation					31:42	glycogen synthase inactivation	13:42	glycogen synthase inactivation	13:42	Mechanism of glycogen synthase inactivation and interaction with glycogenin.
35690592	2	16	with	interaction	218:228	arg1	GS					273:274	GS	273:274	GS	273:274	Glycogenesis involves interaction of glycogenin (GN) with glycogen synthase (GS), where GS is activated by glucose-6-phosphate (G6P) and inactivated by phosphorylation.
35690592	2	16	with	interaction	218:228	arg1	synthase					263:270	glycogen synthase	254:270	glycogen synthase (GS)	254:275	Glycogenesis involves interaction of glycogenin (GN) with glycogen synthase (GS), where GS is activated by glucose-6-phosphate (G6P) and inactivated by phosphorylation.
35690592	6	17	theme	Structure-guided	761:776	arg1	mutagenesis					778:788	Structure-guided mutagenesis	761:788	Structure-guided mutagenesis perturbing interactions with phosphorylated tails	761:838	Structure-guided mutagenesis perturbing interactions with phosphorylated tails led to increased basal/unstimulated GS activity.
35690592	1	18	theme	glucose	99:105	arg1	Glycogen					77:84	Glycogen	77:84	Glycogen	77:84	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	1	18	theme	glucose	99:105	arg1	reserve					107:113	the major glucose reserve	89:113	the major glucose reserve in eukaryotes	89:127	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	4	19	theme	GS	620:621	arg1	helices					634:640	GS regulatory helices	620:640	GS regulatory helices	620:640	Phosphorylated N- and C-termini from two GS protomers converge near the G6P-binding pocket and buttress against GS regulatory helices.
35690592	0	20	theme	glycogen	13:20	arg1	inactivation					31:42	glycogen synthase inactivation	13:42	glycogen synthase inactivation	13:42	Mechanism of glycogen synthase inactivation and interaction with glycogenin.
35690592	4	21	theme	GS	549:550	arg1	protomers					552:560	two GS protomers	545:560	two GS protomers	545:560	Phosphorylated N- and C-termini from two GS protomers converge near the G6P-binding pocket and buttress against GS regulatory helices.
35690592	8	22	theme	synthesis	1121:1129	arg1	regulation					1131:1140	glycogen synthesis regulation	1112:1140	glycogen synthesis regulation	1112:1140	This work therefore provides insights into glycogen synthesis regulation and facilitates studies of glycogen-related diseases.
35690592	5	23	with	interactions	712:723	arg1	"					758:758	a composite "arginine cradle"	730:758	a composite "arginine cradle"	730:758	This keeps GS in an inactive conformation mediated by phospho-Ser641 interactions with a composite "arginine cradle".
35690592	3	24	theme	2.6 Å	381:385	arg1	structure					406:414	the 2.6 Å resolution cryo-EM structure	377:414	the 2.6 Å resolution cryo-EM structure of phosphorylated human GS	377:441	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	5	25	theme	phospho-Ser641	697:710	arg1	interactions					712:723	phospho-Ser641 interactions	697:723	phospho-Ser641 interactions with a composite "arginine cradle"	697:758	This keeps GS in an inactive conformation mediated by phospho-Ser641 interactions with a composite "arginine cradle".
35690592	3	26	theme	human	434:438	arg1	GS					440:441	phosphorylated human GS	419:441	phosphorylated human GS	419:441	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	0	27	theme	inactivation	31:42	arg1	Mechanism					0:8	Mechanism	0:8	Mechanism of glycogen synthase inactivation	0:42	Mechanism of glycogen synthase inactivation and interaction with glycogenin.
35690592	0	27	theme	inactivation	31:42	arg1	interaction					48:58	interaction	48:58	interaction with glycogenin	48:74	Mechanism of glycogen synthase inactivation and interaction with glycogenin.
35690592	4	28	theme	Phosphorylated	508:521	arg1	N-					523:524	Phosphorylated N-	508:524	Phosphorylated N-	508:524	Phosphorylated N- and C-termini from two GS protomers converge near the G6P-binding pocket and buttress against GS regulatory helices.
35690592	3	29	theme	resolution	387:396	arg1	structure					406:414	the 2.6 Å resolution cryo-EM structure	377:414	the 2.6 Å resolution cryo-EM structure of phosphorylated human GS	377:441	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	2	30	theme	glycogenin	233:242	arg1	interaction					218:228	interaction	218:228	interaction of glycogenin (GN) with glycogen synthase (GS), where GS is activated by glucose-6-phosphate (G6P) and inactivated by phosphorylation	218:362	Glycogenesis involves interaction of glycogenin (GN) with glycogen synthase (GS), where GS is activated by glucose-6-phosphate (G6P) and inactivated by phosphorylation.
35690592	7	31	theme	GS	1056:1057	arg1	activity					1059:1066	GS activity	1056:1066	GS activity	1056:1066	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	3	32	theme	GS	440:441	arg1	structure					406:414	the 2.6 Å resolution cryo-EM structure	377:414	the 2.6 Å resolution cryo-EM structure of phosphorylated human GS	377:441	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	6	33	with	interactions	801:812	arg1	tails					834:838	phosphorylated tails	819:838	phosphorylated tails	819:838	Structure-guided mutagenesis perturbing interactions with phosphorylated tails led to increased basal/unstimulated GS activity.
35690592	8	34	theme	diseases	1186:1193	arg1	studies					1158:1164	studies	1158:1164	studies of glycogen-related diseases	1158:1193	This work therefore provides insights into glycogen synthesis regulation and facilitates studies of glycogen-related diseases.
35690592	6	35	theme	GS	876:877	arg1	activity					879:886	increased basal/unstimulated GS activity	847:886	increased basal/unstimulated GS activity	847:886	Structure-guided mutagenesis perturbing interactions with phosphorylated tails led to increased basal/unstimulated GS activity.
35690592	5	36	theme	inactive	663:670	arg1	conformation					672:683	an inactive conformation	660:683	an inactive conformation mediated by phospho-Ser641 interactions with a composite "arginine cradle"	660:758	This keeps GS in an inactive conformation mediated by phospho-Ser641 interactions with a composite "arginine cradle".
35690592	6	37	theme	basal/unstimulated	857:874	arg1	activity					879:886	increased basal/unstimulated GS activity	847:886	increased basal/unstimulated GS activity	847:886	Structure-guided mutagenesis perturbing interactions with phosphorylated tails led to increased basal/unstimulated GS activity.
35690592	2	38	theme	glycogen	254:261	arg1	GS					273:274	GS	273:274	GS	273:274	Glycogenesis involves interaction of glycogenin (GN) with glycogen synthase (GS), where GS is activated by glucose-6-phosphate (G6P) and inactivated by phosphorylation.
35690592	2	38	theme	glycogen	254:261	arg1	synthase					263:270	glycogen synthase	254:270	glycogen synthase (GS)	254:275	Glycogenesis involves interaction of glycogenin (GN) with glycogen synthase (GS), where GS is activated by glucose-6-phosphate (G6P) and inactivated by phosphorylation.
35690592	0	39	with	Mechanism	0:8	arg1	glycogenin					65:74	glycogenin	65:74	glycogenin	65:74	Mechanism of glycogen synthase inactivation and interaction with glycogenin.
35690592	3	40	theme	autoinhibited	456:468	arg1	tetramer					473:480	an autoinhibited GS tetramer	453:480	an autoinhibited GS tetramer flanked by two GN dimers	453:505	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	1	41	from	reserve	107:113	arg1	eukaryotes					118:127	eukaryotes	118:127	eukaryotes	118:127	Glycogen is the major glucose reserve in eukaryotes, and defects in glycogen metabolism and structure lead to disease.
35690592	8	42	theme	glycogen-related	1169:1184	arg1	diseases					1186:1193	glycogen-related diseases	1169:1193	glycogen-related diseases	1169:1193	This work therefore provides insights into glycogen synthesis regulation and facilitates studies of glycogen-related diseases.
35690592	4	43	dep	pocket	592:597	arg1	the					576:578	the	576:578	the	576:578	Phosphorylated N- and C-termini from two GS protomers converge near the G6P-binding pocket and buttress against GS regulatory helices.
35690592	7	44	theme	tuneable	1023:1030	arg1	rheostat					1032:1039	a tuneable rheostat	1021:1039	a tuneable rheostat for regulating GS activity	1021:1066	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	3	45	theme	GS	470:471	arg1	tetramer					473:480	an autoinhibited GS tetramer	453:480	an autoinhibited GS tetramer flanked by two GN dimers	453:505	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	7	46	theme	GS	942:943	arg1	autoinhibition					945:958	GS autoinhibition	942:958	GS autoinhibition	942:958	We propose that multivalent phosphorylation supports GS autoinhibition through interactions from a dynamic "spike" region, allowing a tuneable rheostat for regulating GS activity.
35690592	4	47	theme	G6P-binding	580:590	arg1	pocket					592:597	G6P-binding pocket	580:597	G6P-binding pocket	580:597	Phosphorylated N- and C-termini from two GS protomers converge near the G6P-binding pocket and buttress against GS regulatory helices.
35690592	5	48	theme	arginine	743:750	arg1	"					758:758	a composite "arginine cradle"	730:758	a composite "arginine cradle"	730:758	This keeps GS in an inactive conformation mediated by phospho-Ser641 interactions with a composite "arginine cradle".
35690592	4	49	theme	regulatory	623:632	arg1	helices					634:640	GS regulatory helices	620:640	GS regulatory helices	620:640	Phosphorylated N- and C-termini from two GS protomers converge near the G6P-binding pocket and buttress against GS regulatory helices.
35690592	0	50	with	interaction	48:58	arg1	glycogenin					65:74	glycogenin	65:74	glycogenin	65:74	Mechanism of glycogen synthase inactivation and interaction with glycogenin.
35690592	3	51	theme	GN	497:498	arg1	dimers					500:505	two GN dimers	493:505	two GN dimers	493:505	We describe the 2.6 Å resolution cryo-EM structure of phosphorylated human GS revealing an autoinhibited GS tetramer flanked by two GN dimers.
35690592	5	52	theme	cradle	752:757	arg1	"					758:758	a composite "arginine cradle"	730:758	a composite "arginine cradle"	730:758	This keeps GS in an inactive conformation mediated by phospho-Ser641 interactions with a composite "arginine cradle".
35538419	0	0	theme	percutaneous	79:90	arg1	intervention					101:112	complex percutaneous coronary intervention	71:112	complex percutaneous coronary intervention	71:112	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	5	1	theme	bleeding	763:770	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	1	theme	bleeding	763:770	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	1	theme	bleeding	763:770	arg1	composite					664:672	a composite	662:672	a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications	662:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	1	theme	bleeding	763:770	arg1	bleeding					763:770	bleeding	763:770	bleeding	763:770	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	1	theme	bleeding	763:770	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	1	theme	bleeding	763:770	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	1	theme	bleeding	763:770	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	1	theme	bleeding	763:770	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	composite					664:672	a composite	662:672	a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications	662:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	bleeding					763:770	bleeding	763:770	bleeding	763:770	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	2	theme	primary	642:648	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	composite					664:672	a composite	662:672	a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications	662:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	bleeding					763:770	bleeding	763:770	bleeding	763:770	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	3	theme	death	689:693	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	9	4	theme	thrombosis	1351:1360	arg1	incidences					1306:1315	no incidences	1303:1315	no incidences of myocardial infarction or stent thrombosis in either group	1303:1376	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	0	5	theme	complex	71:77	arg1	intervention					101:112	complex percutaneous coronary intervention	71:112	complex percutaneous coronary intervention	71:112	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	4	6	dep	group	544:548	arg1	group					573:577	non-protamine group	559:577	non-protamine group	559:577	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	10	7	theme	transfemoral	1436:1447	arg1	access					1449:1454	transfemoral access	1436:1454	transfemoral access	1436:1454	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	8	8	dep	had	1116:1118	arg1	4.8 ± 3.7 days					1188:1201	4.8 ± 3.7 days	1188:1201	4.8 ± 3.7 days	1188:1201	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	8	8	dep	had	1116:1118	arg1	8.4 ± 8.3 days					1207:1220	8.4 ± 8.3 days	1207:1220	8.4 ± 8.3 days	1207:1220	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	5	9	theme	stent	719:723	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	10	10	theme	immediate	1457:1465	arg1	administration					1477:1490	immediate protamine administration	1457:1490	immediate protamine administration	1457:1490	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	3	11	dep	safety	383:388	arg1	the					379:381	the	379:381	the	379:381	This study aimed to evaluate the safety and efficacy of manual compression with and without protamine after transfemoral complex PCI.
35538419	10	12	theme	vascular	1542:1549	arg1	complications					1558:1570	vascular access complications	1542:1570	vascular access complications	1542:1570	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	10	12	theme	vascular	1542:1549	arg1	hematoma					1584:1591	hematoma	1584:1591	hematoma	1584:1591	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	2	13	with	PCI	314:316	arg1	technologies					336:347	contemporary technologies	323:347	contemporary technologies	323:347	No previous study has evaluated the use of protamine after PCI with contemporary technologies.
35538419	0	14	theme	coronary	92:99	arg1	intervention					101:112	complex percutaneous coronary intervention	71:112	complex percutaneous coronary intervention	71:112	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	9	15	theme	coronary	1273:1280	arg1	syndrome					1282:1289	acute coronary syndrome	1267:1289	acute coronary syndrome	1267:1289	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	8	16	contain	had	1116:1118	arg2	hospital					1144:1151	a significantly shorter hospital	1120:1151	a significantly shorter hospital stay than the non-protamine group	1120:1185	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	8	16	contain	had	1116:1118	arg1	group					1110:1114	the protamine group	1096:1114	the protamine group	1096:1114	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	7	17	dep	driven	981:986	arg1	%					1058:1058	3.3%	1055:1058	3.3%	1055:1058	This was driven mainly by the lower incidences of hematoma in the protamine group (3.3% vs. 13.2%, p = 0.020).
35538419	7	17	dep	driven	981:986	arg1	%					1068:1068	13.2%	1064:1068	13.2%	1064:1068	This was driven mainly by the lower incidences of hematoma in the protamine group (3.3% vs. 13.2%, p = 0.020).
35538419	9	18	theme	patients	1254:1261	arg1	%					1245:1245	While > 90%	1235:1245	While > 90% of the patients	1235:1261	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	9	18	theme	patients	1254:1261	arg1	patients					1254:1261	the patients	1250:1261	the patients	1250:1261	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	10	19	theme	no	1625:1626	arg1	administration					1638:1651	no protamine administration	1625:1651	no protamine administration	1625:1651	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	5	20	theme	stroke/systemic	737:751	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	6	21	theme	protamine	895:903	arg1	group					905:909	the protamine group	891:909	the protamine group	891:909	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	0	22	theme	heparin	33:39	arg1	reversal					41:48	immediate heparin reversal	23:48	immediate heparin reversal	23:48	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	4	23	theme	complex	602:608	arg1	PCI					610:612	complex PCI	602:612	complex PCI	602:612	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	7	24	theme	lower	1002:1006	arg1	incidences					1008:1017	the lower incidences	998:1017	the lower incidences of hematoma	998:1029	This was driven mainly by the lower incidences of hematoma in the protamine group (3.3% vs. 13.2%, p = 0.020).
35538419	5	25	theme	vascular	805:812	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	9	26	theme	acute	1267:1271	arg1	syndrome					1282:1289	acute coronary syndrome	1267:1289	acute coronary syndrome	1267:1289	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	4	27	dep	retrospectively	495:509	arg1	analyzed					511:518	analyzed	511:518	retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery	495:635	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	1	28	theme	complex	187:193	arg1	intervention					167:178	simple percutaneous coronary intervention	138:178	simple percutaneous coronary intervention (PCI)	138:184	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	28	theme	complex	187:193	arg1	PCI					195:197	complex PCI	187:197	complex PCI	187:197	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	5	29	theme	access	814:819	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	4	30	theme	non-protamine	559:571	arg1	group					573:577	non-protamine group	559:577	non-protamine group	559:577	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	8	31	dep	4.8 ± 3.7 days	1188:1201	arg1	p = 0.001					1223:1231	p = 0.001	1223:1231	p = 0.001	1223:1231	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	5	32	theme	complications	821:833	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	32	theme	complications	821:833	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	32	theme	complications	821:833	arg1	composite					664:672	a composite	662:672	a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications	662:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	32	theme	complications	821:833	arg1	bleeding					763:770	bleeding	763:770	bleeding	763:770	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	32	theme	complications	821:833	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	32	theme	complications	821:833	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	32	theme	complications	821:833	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	32	theme	complications	821:833	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	6	33	dep	RESULTS	836:842	arg1	outcome					856:862	The primary outcome	844:862	The primary outcome	844:862	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	6	33	dep	RESULTS	836:842	arg1	lower					882:886	lower	882:886	lower	882:886	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	7	34	theme	protamine	1038:1046	arg1	group					1048:1052	the protamine group	1034:1052	the protamine group	1034:1052	This was driven mainly by the lower incidences of hematoma in the protamine group (3.3% vs. 13.2%, p = 0.020).
35538419	2	35	theme	protamine	298:306	arg1	use					291:293	the use	287:293	the use of protamine	287:306	No previous study has evaluated the use of protamine after PCI with contemporary technologies.
35538419	9	36	contain	had	1263:1265	arg1	%					1245:1245	While > 90%	1235:1245	While > 90% of the patients	1235:1261	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	9	36	contain	had	1263:1265	arg1	patients					1254:1261	the patients	1250:1261	the patients	1250:1261	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	9	36	contain	had	1263:1265	arg2	syndrome					1282:1289	acute coronary syndrome	1267:1289	acute coronary syndrome	1267:1289	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	3	37	theme	complex	471:477	arg1	PCI					479:481	transfemoral complex PCI	458:481	transfemoral complex PCI	458:481	This study aimed to evaluate the safety and efficacy of manual compression with and without protamine after transfemoral complex PCI.
35538419	8	38	theme	shorter	1136:1142	arg1	hospital					1144:1151	a significantly shorter hospital	1120:1151	a significantly shorter hospital stay than the non-protamine group	1120:1185	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	4	39	dep	METHODS	484:490	arg1	retrospectively					495:509	retrospectively	495:509	retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery	495:635	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	5	40	theme	myocardial	696:705	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	6	41	theme	non-protamine	923:935	arg1	group					937:941	the non-protamine group	919:941	the non-protamine group	919:941	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	4	42	theme	femoral	622:628	arg1	artery					630:635	the femoral artery	618:635	the femoral artery	618:635	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	3	43	theme	manual	406:411	arg1	compression					413:423	manual compression	406:423	manual compression	406:423	This study aimed to evaluate the safety and efficacy of manual compression with and without protamine after transfemoral complex PCI.
35538419	5	44	theme	infarction	707:716	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	44	theme	infarction	707:716	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	44	theme	infarction	707:716	arg1	composite					664:672	a composite	662:672	a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications	662:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	44	theme	infarction	707:716	arg1	bleeding					763:770	bleeding	763:770	bleeding	763:770	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	44	theme	infarction	707:716	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	44	theme	infarction	707:716	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	44	theme	infarction	707:716	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	44	theme	infarction	707:716	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	10	45	theme	access	1551:1556	arg1	complications					1558:1570	vascular access complications	1542:1570	vascular access complications	1542:1570	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	10	45	theme	access	1551:1556	arg1	hematoma					1584:1591	hematoma	1584:1591	hematoma	1584:1591	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	0	46	theme	immediate	23:31	arg1	reversal					41:48	immediate heparin reversal	23:48	immediate heparin reversal	23:48	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	3	47	theme	compression	413:423	arg1	safety					383:388	safety	383:388	safety	383:388	This study aimed to evaluate the safety and efficacy of manual compression with and without protamine after transfemoral complex PCI.
35538419	3	47	theme	compression	413:423	arg1	efficacy					394:401	efficacy	394:401	efficacy	394:401	This study aimed to evaluate the safety and efficacy of manual compression with and without protamine after transfemoral complex PCI.
35538419	1	48	dep	bleeding	225:232	arg1	higher					218:223	higher	218:223	higher	218:223	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	10	49	theme	complications	1558:1570	arg1	rate					1534:1537	a significantly lower rate	1512:1537	a significantly lower rate of vascular access complications, especially hematoma	1512:1591	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	3	50	theme	transfemoral	458:469	arg1	PCI					479:481	transfemoral complex PCI	458:481	transfemoral complex PCI	458:481	This study aimed to evaluate the safety and efficacy of manual compression with and without protamine after transfemoral complex PCI.
35538419	7	51	dep	%	1058:1058	arg1	p = 0.020					1071:1079	p = 0.020	1071:1079	p = 0.020	1071:1079	This was driven mainly by the lower incidences of hematoma in the protamine group (3.3% vs. 13.2%, p = 0.020).
35538419	4	52	theme	protamine	534:542	arg1	n = 68					580:585	n = 68	580:585	n = 68	580:585	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	4	52	theme	protamine	534:542	arg1	n = 92					551:556	n = 92	551:556	n = 92	551:556	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	4	52	theme	protamine	534:542	arg1	group					544:548	protamine group	534:548	protamine group	534:548	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	8	53	dep	hospital	1144:1151	arg1	stay					1153:1156	stay	1153:1156	stay than the non-protamine group	1153:1185	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	6	54	dep	%	947:947	arg1	p = 0.006					960:968	p = 0.006	960:968	p = 0.006	960:968	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	0	55	with	efficacy	11:18	arg1	protamine					55:63	protamine	55:63	protamine after complex percutaneous coronary intervention	55:112	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	0	56	theme	reversal	41:48	arg1	efficacy					11:18	efficacy	11:18	efficacy	11:18	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	0	56	theme	reversal	41:48	arg1	Safety					0:5	Safety	0:5	Safety	0:5	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	7	57	theme	hematoma	1022:1029	arg1	incidences					1008:1017	the lower incidences	998:1017	the lower incidences of hematoma	998:1029	This was driven mainly by the lower incidences of hematoma in the protamine group (3.3% vs. 13.2%, p = 0.020).
35538419	9	58	from	incidences	1306:1315	arg1	group					1372:1376	either group	1365:1376	either group	1365:1376	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	10	59	theme	complex	1420:1426	arg1	PCI					1428:1430	complex PCI	1420:1430	complex PCI	1420:1430	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	10	60	theme	lower	1528:1532	arg1	rate					1534:1537	a significantly lower rate	1512:1537	a significantly lower rate of vascular access complications, especially hematoma	1512:1591	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	10	61	theme	shorter	1598:1604	arg1	hospital					1606:1613	shorter hospital	1598:1613	shorter hospital	1598:1613	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	6	62	dep	lower	882:886	arg1	%					947:947	4.3%	944:947	4.3%	944:947	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	6	62	dep	lower	882:886	arg1	%					957:957	17.6%	953:957	17.6%	953:957	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	5	63	theme	in-hospital	677:687	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	6	64	theme	primary	848:854	arg1	outcome					856:862	The primary outcome	844:862	The primary outcome	844:862	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	6	64	theme	primary	848:854	arg1	lower					882:886	lower	882:886	lower	882:886	RESULTS The primary outcome was significantly lower in the protamine group than in the non-protamine group (4.3% vs. 17.6%; p = 0.006).
35538419	0	65	with	Safety	0:5	arg1	protamine					55:63	protamine	55:63	protamine after complex percutaneous coronary intervention	55:112	Safety and efficacy of immediate heparin reversal with protamine after complex percutaneous coronary intervention.
35538419	1	66	theme	simple	138:143	arg1	intervention					167:178	simple percutaneous coronary intervention	138:178	simple percutaneous coronary intervention (PCI)	138:184	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	66	theme	simple	138:143	arg1	PCI					195:197	complex PCI	187:197	complex PCI	187:197	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	66	theme	simple	138:143	arg1	PCI					181:183	PCI	181:183	PCI	181:183	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	8	67	theme	protamine	1100:1108	arg1	group					1110:1114	the protamine group	1096:1114	the protamine group	1096:1114	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	5	68	theme	blood	782:786	arg1	transfusion					788:798	blood transfusion	782:798	blood transfusion	782:798	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	9	69	theme	infarction	1331:1340	arg1	incidences					1306:1315	no incidences	1303:1315	no incidences of myocardial infarction or stent thrombosis in either group	1303:1376	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	2	70	theme	previous	258:265	arg1	study					267:271	No previous study	255:271	No previous study	255:271	No previous study has evaluated the use of protamine after PCI with contemporary technologies.
35538419	10	71	theme	protamine	1628:1636	arg1	administration					1638:1651	no protamine administration	1625:1651	no protamine administration	1625:1651	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	1	72	theme	percutaneous	145:156	arg1	intervention					167:178	simple percutaneous coronary intervention	138:178	simple percutaneous coronary intervention (PCI)	138:184	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	72	theme	percutaneous	145:156	arg1	PCI					195:197	complex PCI	187:197	complex PCI	187:197	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	72	theme	percutaneous	145:156	arg1	PCI					181:183	PCI	181:183	PCI	181:183	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	2	73	theme	contemporary	323:334	arg1	technologies					336:347	contemporary technologies	323:347	contemporary technologies	323:347	No previous study has evaluated the use of protamine after PCI with contemporary technologies.
35538419	5	74	theme	embolism	753:760	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	74	theme	embolism	753:760	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	74	theme	embolism	753:760	arg1	composite					664:672	a composite	662:672	a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications	662:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	74	theme	embolism	753:760	arg1	bleeding					763:770	bleeding	763:770	bleeding	763:770	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	74	theme	embolism	753:760	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	74	theme	embolism	753:760	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	74	theme	embolism	753:760	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	74	theme	embolism	753:760	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	infarction					707:716	myocardial infarction	696:716	myocardial infarction	696:716	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	embolism					753:760	stroke/systemic embolism	737:760	stroke/systemic embolism	737:760	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	composite					664:672	a composite	662:672	a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications	662:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	bleeding					763:770	bleeding	763:770	bleeding	763:770	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	death					689:693	in-hospital death	677:693	in-hospital death	677:693	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	complications					821:833	vascular access complications	805:833	vascular access complications	805:833	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	thrombosis					725:734	stent thrombosis	719:734	stent thrombosis	719:734	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	5	75	theme	thrombosis	725:734	arg1	outcome					650:656	The primary outcome	638:656	The primary outcome	638:656	The primary outcome was a composite of in-hospital death, myocardial infarction, stent thrombosis, stroke/systemic embolism, bleeding requiring blood transfusion, and vascular access complications.
35538419	10	76	theme	protamine	1467:1475	arg1	administration					1477:1490	immediate protamine administration	1457:1490	immediate protamine administration	1457:1490	CONCLUSIONS Among patients who underwent complex PCI via transfemoral access, immediate protamine administration was associated with a significantly lower rate of vascular access complications, especially hematoma, and shorter hospital stay than no protamine administration.
35538419	1	77	theme	coronary	158:165	arg1	intervention					167:178	simple percutaneous coronary intervention	138:178	simple percutaneous coronary intervention (PCI)	138:184	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	77	theme	coronary	158:165	arg1	PCI					195:197	complex PCI	187:197	complex PCI	187:197	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	77	theme	coronary	158:165	arg1	PCI					181:183	PCI	181:183	PCI	181:183	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	1	78	theme	thrombotic	238:247	arg1	risk					249:252	higher bleeding and thrombotic risk	218:252	risk	249:252	BACKGROUND Compared to simple percutaneous coronary intervention (PCI), complex PCI is associated with higher bleeding and thrombotic risk.
35538419	8	79	theme	non-protamine	1167:1179	arg1	group					1181:1185	the non-protamine group	1163:1185	the non-protamine group	1163:1185	Furthermore, the protamine group had a significantly shorter hospital stay than the non-protamine group (4.8 ± 3.7 days vs. 8.4 ± 8.3 days, p = 0.001).
35538419	9	80	theme	stent	1345:1349	arg1	thrombosis					1351:1360	stent thrombosis	1345:1360	stent thrombosis	1345:1360	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	9	81	theme	myocardial	1320:1329	arg1	infarction					1331:1340	myocardial infarction	1320:1340	myocardial infarction	1320:1340	While > 90% of the patients had acute coronary syndrome, there were no incidences of myocardial infarction or stent thrombosis in either group.
35538419	4	82	dep	patients	524:531	arg1	n = 68					580:585	n = 68	580:585	n = 68	580:585	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	4	82	dep	patients	524:531	arg1	n = 92					551:556	n = 92	551:556	n = 92	551:556	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
35538419	4	82	dep	patients	524:531	arg1	group					544:548	protamine group	534:548	protamine group	534:548	METHODS We retrospectively analyzed 160 patients (protamine group, n = 92; non-protamine group, n = 68) who underwent complex PCI via the femoral artery.
36052783	10	0	theme	main	1559:1562	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	0	theme	main	1559:1562	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	0	theme	main	1559:1562	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	0	theme	main	1559:1562	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	0	theme	main	1559:1562	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	0	theme	main	1559:1562	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	11	1	theme	soil	1838:1841	arg1	habitat					1843:1849	soil habitat	1838:1849	soil habitat	1838:1849	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	7	2	theme	bacterial	1114:1122	arg1	communities					1135:1145	bacterial and fungal communities	1114:1145	bacterial and fungal communities	1114:1145	The diversity and richness of bacterial and fungal communities significantly increased after litter decomposition.
36052783	1	3	theme	microbial	349:357	arg1	communities					359:369	soil microbial communities	344:369	soil microbial communities	344:369	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	4	theme	Eragrostis	145:154	arg1	mosses					163:168	mosses	163:168	mosses	163:168	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	4	theme	Eragrostis	145:154	arg1	characteristics					126:140	the decomposition characteristics	108:140	the decomposition characteristics of Eragrostis minor	108:160	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	9	5	theme	communities	1433:1443	arg1	rate					1373:1376	decomposition rate	1359:1376	decomposition rate	1359:1376	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36052783	9	5	theme	communities	1433:1443	arg1	diversity					1386:1394	diversity	1386:1394	diversity	1386:1394	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36052783	9	5	theme	communities	1433:1443	arg1	richness					1400:1407	richness	1400:1407	richness	1400:1407	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36052783	0	6	theme	Shapotou	69:76	arg1	area					78:81	Shapotou area	69:81	Shapotou area	69:81	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	0	7	from	decomposition	8:20	arg1	community					56:64	soil microbial community	41:64	soil microbial community in Shapotou area	41:81	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	0	8	from	community	56:64	arg1	area					78:81	Shapotou area	69:81	Shapotou area	69:81	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	10	9	theme	microbial	1582:1590	arg1	structure					1602:1610	microbial community structure	1582:1610	microbial community structure	1582:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	1	10	theme	sand-binding	233:244	arg1	edge					278:281	southeastern edge	265:281	southeastern edge	265:281	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	10	theme	sand-binding	233:244	arg1	area					259:262	the sand-binding revegetation area	229:262	the sand-binding revegetation area	229:262	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	11	11	theme	microbial	1694:1702	arg1	communities					1704:1714	microbial communities	1694:1714	microbial communities	1694:1714	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	10	12	theme	biomass	1506:1512	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	12	theme	biomass	1506:1512	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	12	theme	biomass	1506:1512	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	12	theme	biomass	1506:1512	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	12	theme	biomass	1506:1512	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	12	theme	biomass	1506:1512	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	4	13	dep	minor	685:689	arg1	E.					682:683	E. minor	682:689	E. minor	682:689	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	1	14	theme	revegetation	246:257	arg1	edge					278:281	southeastern edge	265:281	southeastern edge	265:281	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	14	theme	revegetation	246:257	arg1	area					259:262	the sand-binding revegetation area	229:262	the sand-binding revegetation area	229:262	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	11	15	theme	communities	1783:1793	arg1	richness					1756:1763	richness	1756:1763	richness	1756:1763	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	11	15	theme	communities	1783:1793	arg1	diversity					1742:1750	diversity	1742:1750	diversity	1742:1750	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	3	16	theme	lowest	548:553	arg1	rate					569:572	the lowest decomposition rate	544:572	the lowest decomposition rate	544:572	Mosses had the lowest decomposition rate, with a mass loss ratio of only 15.4% after decomposition for 13 months.
36052783	11	17	dep	diversity	1742:1750	arg1	the					1738:1740	the	1738:1740	the	1738:1740	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	2	18	theme	decomposition	513:525	arg1	rate					527:530	litter decomposition rate	506:530	litter decomposition rate	506:530	The results showed that the decomposition duration and litter types significantly affected litter decomposition rate.
36052783	10	19	theme	microbial	1496:1504	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	19	theme	microbial	1496:1504	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	19	theme	microbial	1496:1504	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	19	theme	microbial	1496:1504	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	19	theme	microbial	1496:1504	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	19	theme	microbial	1496:1504	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	0	20	dep	China	84:88	arg1	decomposition					8:20	[Litter decomposition	0:20	[Litter decomposition	0:20	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	0	20	dep	China	84:88	arg1	effects					30:36	its effects	26:36	its effects on soil microbial community in Shapotou area	26:81	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	5	21	theme	dominant	812:819	arg1	Actinomycota					842:853	Actinomycota	842:853	Actinomycota	842:853	During decomposition for 11 months, the dominant bacterial phyla were Actinomycota and Proteobacteria, while that of the fungal community was Ascomycota.
36052783	5	21	theme	dominant	812:819	arg1	phyla					831:835	the dominant bacterial phyla	808:835	the dominant bacterial phyla	808:835	During decomposition for 11 months, the dominant bacterial phyla were Actinomycota and Proteobacteria, while that of the fungal community was Ascomycota.
36052783	2	22	theme	litter	506:511	arg1	rate					527:530	litter decomposition rate	506:530	litter decomposition rate	506:530	The results showed that the decomposition duration and litter types significantly affected litter decomposition rate.
36052783	1	23	theme	soil	344:347	arg1	communities					359:369	soil microbial communities	344:369	soil microbial communities	344:369	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	7	24	theme	fungal	1128:1133	arg1	communities					1135:1145	bacterial and fungal communities	1114:1145	bacterial and fungal communities	1114:1145	The diversity and richness of bacterial and fungal communities significantly increased after litter decomposition.
36052783	9	25	theme	bacterial	1412:1420	arg1	communities					1433:1443	bacterial and fungal communities	1412:1443	bacterial and fungal communities	1412:1443	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36052783	7	26	dep	diversity	1088:1096	arg1	The					1084:1086	The	1084:1086	The	1084:1086	The diversity and richness of bacterial and fungal communities significantly increased after litter decomposition.
36052783	5	27	theme	bacterial	821:829	arg1	Actinomycota					842:853	Actinomycota	842:853	Actinomycota	842:853	During decomposition for 11 months, the dominant bacterial phyla were Actinomycota and Proteobacteria, while that of the fungal community was Ascomycota.
36052783	5	27	theme	bacterial	821:829	arg1	phyla					831:835	the dominant bacterial phyla	808:835	the dominant bacterial phyla	808:835	During decomposition for 11 months, the dominant bacterial phyla were Actinomycota and Proteobacteria, while that of the fungal community was Ascomycota.
36052783	8	28	theme	compositional	1203:1215	arg1	changes					1217:1223	The compositional changes	1199:1223	The compositional changes of fungal community	1199:1243	The compositional changes of fungal community were significant among litters, but that of bacterial community was not.
36052783	8	28	theme	compositional	1203:1215	arg1	significant					1250:1260	significant	1250:1260	significant	1250:1260	The compositional changes of fungal community were significant among litters, but that of bacterial community was not.
36052783	0	29	from	effects	30:36	arg1	community					56:64	soil microbial community	41:64	soil microbial community in Shapotou area	41:81	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	10	30	theme	Plant	1446:1450	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	30	theme	Plant	1446:1450	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	30	theme	Plant	1446:1450	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	30	theme	Plant	1446:1450	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	30	theme	Plant	1446:1450	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	30	theme	Plant	1446:1450	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	5	31	theme	fungal	893:898	arg1	community					900:908	the fungal community	889:908	the fungal community	889:908	During decomposition for 11 months, the dominant bacterial phyla were Actinomycota and Proteobacteria, while that of the fungal community was Ascomycota.
36052783	4	32	theme	average	651:657	arg1	4.9					722:724	4.9	722:724	4.9	722:724	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	32	theme	average	651:657	arg1	rates					673:677	The average decomposition rates	647:677	The average decomposition rates of E. minor	647:689	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	32	theme	average	651:657	arg1	that					742:745	that	742:745	that	742:745	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	0	33	theme	[Litter	0:6	arg1	decomposition					8:20	[Litter decomposition	0:20	[Litter decomposition	0:20	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	10	34	theme	soil	1487:1490	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	34	theme	soil	1487:1490	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	34	theme	soil	1487:1490	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	34	theme	soil	1487:1490	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	34	theme	soil	1487:1490	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	34	theme	soil	1487:1490	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	11	35	theme	microbial	1773:1781	arg1	communities					1783:1793	soil microbial communities	1768:1793	soil microbial communities	1768:1793	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	11	36	dep	composition	1646:1656	arg1	the					1642:1644	the	1642:1644	the	1642:1644	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	1	37	from	effects	333:339	arg1	communities					359:369	soil microbial communities	344:369	soil microbial communities	344:369	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	38	theme	southeastern	265:276	arg1	edge					278:281	southeastern edge	265:281	southeastern edge	265:281	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	38	theme	southeastern	265:276	arg1	area					259:262	the sand-binding revegetation area	229:262	the sand-binding revegetation area	229:262	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	10	39	theme	total	1469:1473	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	39	theme	total	1469:1473	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	39	theme	total	1469:1473	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	39	theme	total	1469:1473	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	39	theme	total	1469:1473	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	39	theme	total	1469:1473	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	3	40	theme	decomposition	555:567	arg1	rate					569:572	the lowest decomposition rate	544:572	the lowest decomposition rate	544:572	Mosses had the lowest decomposition rate, with a mass loss ratio of only 15.4% after decomposition for 13 months.
36052783	7	41	theme	litter	1177:1182	arg1	decomposition					1184:1196	litter decomposition	1177:1196	litter decomposition	1177:1196	The diversity and richness of bacterial and fungal communities significantly increased after litter decomposition.
36052783	6	42	theme	Moss	926:929	arg1	decomposition					931:943	Moss decomposition	926:943	Moss decomposition	926:943	Moss decomposition significantly increased the relative abundance of Bacteroidetes and Chloroflexi, but remarkedly decreased the abundance of Basidiomycetes.
36052783	4	43	theme	that	742:745	arg1	4.9					722:724	4.9	722:724	4.9	722:724	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	43	theme	that	742:745	arg1	rates					673:677	The average decomposition rates	647:677	The average decomposition rates of E. minor	647:689	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	43	theme	that	742:745	arg1	that					742:745	that	742:745	that	742:745	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	1	44	theme	minor	156:160	arg1	Eragrostis					145:154	Eragrostis minor	145:160	Eragrostis minor	145:160	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	8	45	theme	community	1235:1243	arg1	changes					1217:1223	The compositional changes	1199:1223	The compositional changes of fungal community	1199:1243	The compositional changes of fungal community were significant among litters, but that of bacterial community was not.
36052783	8	45	theme	community	1235:1243	arg1	significant					1250:1260	significant	1250:1260	significant	1250:1260	The compositional changes of fungal community were significant among litters, but that of bacterial community was not.
36052783	11	46	theme	habitat	1843:1849	arg1	restoration					1823:1833	the restoration	1819:1833	the restoration of soil habitat	1819:1849	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	3	47	theme	loss	587:590	arg1	ratio					592:596	a mass loss ratio	580:596	a mass loss ratio of only 15.4% after decomposition for 13 months	580:644	Mosses had the lowest decomposition rate, with a mass loss ratio of only 15.4% after decomposition for 13 months.
36052783	2	48	theme	litter	470:475	arg1	types					477:481	litter types	470:481	litter types	470:481	The results showed that the decomposition duration and litter types significantly affected litter decomposition rate.
36052783	1	49	theme	Artemisia	185:193	arg1	ordosica					195:202	Artemisia ordosica	185:202	Artemisia ordosica	185:202	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	6	50	theme	Basidiomycetes	1068:1081	arg1	abundance					1055:1063	the abundance	1051:1063	the abundance of Basidiomycetes	1051:1081	Moss decomposition significantly increased the relative abundance of Bacteroidetes and Chloroflexi, but remarkedly decreased the abundance of Basidiomycetes.
36052783	3	51	contain	had	540:542	arg1	Mosses					533:538	Mosses	533:538	Mosses	533:538	Mosses had the lowest decomposition rate, with a mass loss ratio of only 15.4% after decomposition for 13 months.
36052783	3	51	contain	had	540:542	arg2	rate					569:572	the lowest decomposition rate	544:572	the lowest decomposition rate	544:572	Mosses had the lowest decomposition rate, with a mass loss ratio of only 15.4% after decomposition for 13 months.
36052783	6	52	theme	relative	973:980	arg1	abundance					982:990	the relative abundance	969:990	the relative abundance of Bacteroidetes	969:1007	Moss decomposition significantly increased the relative abundance of Bacteroidetes and Chloroflexi, but remarkedly decreased the abundance of Basidiomycetes.
36052783	9	53	theme	decomposition	1359:1371	arg1	rate					1373:1376	decomposition rate	1359:1376	decomposition rate	1359:1376	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36052783	1	54	theme	ordosica	195:202	arg1	mosses					163:168	mosses	163:168	mosses	163:168	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	54	theme	ordosica	195:202	arg1	characteristics					126:140	the decomposition characteristics	108:140	the decomposition characteristics of Eragrostis minor	108:160	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	0	55	theme	microbial	46:54	arg1	community					56:64	soil microbial community	41:64	soil microbial community in Shapotou area	41:81	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	1	56	theme	Tengger	290:296	arg1	Desert					298:303	the Tengger Desert	286:303	the Tengger Desert	286:303	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	8	57	theme	fungal	1228:1233	arg1	community					1235:1243	fungal community	1228:1243	fungal community	1228:1243	The compositional changes of fungal community were significant among litters, but that of bacterial community was not.
36052783	1	58	theme	Illumina	381:388	arg1	method					407:412	the Illumina MiSeq sequencing method	377:412	the Illumina MiSeq sequencing method	377:412	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	9	59	theme	negative	1330:1337	arg1	correlation					1339:1349	a negative correlation	1328:1349	a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities	1328:1443	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36052783	0	60	theme	soil	41:44	arg1	community					56:64	soil microbial community	41:64	soil microbial community in Shapotou area	41:81	[Litter decomposition and its effects on soil microbial community in Shapotou area, China].
36052783	1	61	theme	Desert	298:303	arg1	edge					278:281	southeastern edge	265:281	southeastern edge	265:281	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	61	theme	Desert	298:303	arg1	area					259:262	the sand-binding revegetation area	229:262	the sand-binding revegetation area	229:262	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	62	theme	MiSeq	390:394	arg1	method					407:412	the Illumina MiSeq sequencing method	377:412	the Illumina MiSeq sequencing method	377:412	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	3	63	theme	mass	582:585	arg1	ratio					592:596	a mass loss ratio	580:596	a mass loss ratio of only 15.4% after decomposition for 13 months	580:644	Mosses had the lowest decomposition rate, with a mass loss ratio of only 15.4% after decomposition for 13 months.
36052783	11	64	theme	Litter	1613:1618	arg1	decomposition					1620:1632	Litter decomposition	1613:1632	Litter decomposition	1613:1632	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	4	65	theme	minor	685:689	arg1	4.9					722:724	4.9	722:724	4.9	722:724	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	65	theme	minor	685:689	arg1	rates					673:677	The average decomposition rates	647:677	The average decomposition rates of E. minor	647:689	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	65	theme	minor	685:689	arg1	that					742:745	that	742:745	that	742:745	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	10	66	theme	ammonium	1533:1540	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	66	theme	ammonium	1533:1540	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	66	theme	ammonium	1533:1540	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	66	theme	ammonium	1533:1540	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	66	theme	ammonium	1533:1540	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	66	theme	ammonium	1533:1540	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	1	67	theme	sequencing	396:405	arg1	method					407:412	the Illumina MiSeq sequencing method	377:412	the Illumina MiSeq sequencing method	377:412	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	7	68	theme	communities	1135:1145	arg1	diversity					1088:1096	diversity	1088:1096	diversity	1088:1096	The diversity and richness of bacterial and fungal communities significantly increased after litter decomposition.
36052783	7	68	theme	communities	1135:1145	arg1	richness					1102:1109	richness	1102:1109	richness	1102:1109	The diversity and richness of bacterial and fungal communities significantly increased after litter decomposition.
36052783	11	69	theme	soil	1768:1771	arg1	communities					1783:1793	soil microbial communities	1768:1793	soil microbial communities	1768:1793	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	10	70	theme	soil	1528:1531	arg1	content					1542:1548	soil ammonium content	1528:1548	soil ammonium content	1528:1548	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	70	theme	soil	1528:1531	arg1	phosphorus					1475:1484	total phosphorus	1469:1484	total phosphorus	1469:1484	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	70	theme	soil	1528:1531	arg1	nitrogen					1514:1521	microbial biomass nitrogen	1496:1521	microbial biomass nitrogen	1496:1521	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	70	theme	soil	1528:1531	arg1	polysaccharides					1452:1466	Plant polysaccharides	1446:1466	Plant polysaccharides	1446:1466	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	70	theme	soil	1528:1531	arg1	factors					1564:1570	the main factors	1555:1570	the main factors affecting microbial community structure	1555:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	10	70	theme	soil	1528:1531	arg1	pH					1492:1493	soil pH	1487:1493	soil pH	1487:1493	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	3	71	theme	%	610:610	arg1	ratio					592:596	a mass loss ratio	580:596	a mass loss ratio of only 15.4% after decomposition for 13 months	580:644	Mosses had the lowest decomposition rate, with a mass loss ratio of only 15.4% after decomposition for 13 months.
36052783	11	72	theme	interspecific	1662:1674	arg1	similarity					1676:1685	interspecific similarity	1662:1685	interspecific similarity	1662:1685	Litter decomposition changed the composition and interspecific similarity within microbial communities, as well as increased the diversity and richness of soil microbial communities, and thus would promote the restoration of soil habitat.
36052783	1	73	theme	decomposition	112:124	arg1	characteristics					126:140	the decomposition characteristics	108:140	the decomposition characteristics of Eragrostis minor	108:160	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	1	74	theme	litterbag	209:217	arg1	method					219:224	litterbag method	209:224	litterbag method	209:224	We investigated the decomposition characteristics of Eragrostis minor, mosses, and leaves of Artemisia ordosica with litterbag method in the sand-binding revegetation area, southeastern edge of the Tengger Desert, and further examined their effects on soil microbial communities using the Illumina MiSeq sequencing method.
36052783	8	75	theme	bacterial	1289:1297	arg1	community					1299:1307	bacterial community	1289:1307	bacterial community	1289:1307	The compositional changes of fungal community were significant among litters, but that of bacterial community was not.
36052783	10	76	theme	community	1592:1600	arg1	structure					1602:1610	microbial community structure	1582:1610	microbial community structure	1582:1610	Plant polysaccharides, total phosphorus, soil pH, microbial biomass nitrogen, and soil ammonium content were the main factors affecting microbial community structure.
36052783	6	77	theme	Bacteroidetes	995:1007	arg1	abundance					982:990	the relative abundance	969:990	the relative abundance of Bacteroidetes	969:1007	Moss decomposition significantly increased the relative abundance of Bacteroidetes and Chloroflexi, but remarkedly decreased the abundance of Basidiomycetes.
36052783	6	77	theme	Bacteroidetes	995:1007	arg1	Chloroflexi					1013:1023	Chloroflexi	1013:1023	Chloroflexi	1013:1023	Moss decomposition significantly increased the relative abundance of Bacteroidetes and Chloroflexi, but remarkedly decreased the abundance of Basidiomycetes.
36052783	2	78	theme	decomposition	443:455	arg1	duration					457:464	the decomposition duration	439:464	the decomposition duration	439:464	The results showed that the decomposition duration and litter types significantly affected litter decomposition rate.
36052783	9	79	dep	diversity	1386:1394	arg1	the					1382:1384	the	1382:1384	the	1382:1384	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36052783	4	80	theme	decomposition	659:671	arg1	4.9					722:724	4.9	722:724	4.9	722:724	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	80	theme	decomposition	659:671	arg1	rates					673:677	The average decomposition rates	647:677	The average decomposition rates of E. minor	647:689	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	4	80	theme	decomposition	659:671	arg1	that					742:745	that	742:745	that	742:745	The average decomposition rates of E. minor and leaves of A. ordosica were 4.9 and 3.4-fold of that of mosses, respectively.
36052783	9	81	theme	fungal	1426:1431	arg1	communities					1433:1443	bacterial and fungal communities	1412:1443	bacterial and fungal communities	1412:1443	There was a negative correlation between decomposition rate and the diversity and richness of bacterial and fungal communities.
36921032	7	0	theme	SLE	1266:1268	arg1	immunopathogenesis					1270:1287	SLE immunopathogenesis	1266:1287	SLE immunopathogenesis	1266:1287	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	5	1	contain	have	819:822	arg1	Mice					793:796	Mice	793:796	Mice	793:796	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	5	1	contain	have	819:822	arg2	abundance					833:841	a higher abundance	824:841	a higher abundance of mannose structures	824:863	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	6	2	theme	T	1150:1150	arg1	infiltration					1157:1168	γδ T cell infiltration	1147:1168	γδ T cell infiltration	1147:1168	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	3	3	theme	protein	358:364	arg1	glycosylation					366:378	protein glycosylation	358:378	protein glycosylation	358:378	Accumulating evidence has demonstrated that protein glycosylation is substantially altered in autoimmune disease development, but the mechanisms by which glycans trigger these autoreactive immune responses are still largely unclear.
36921032	5	4	theme	higher	826:831	arg1	abundance					833:841	a higher abundance	824:841	a higher abundance of mannose structures	824:863	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	6	5	theme	cell	1152:1155	arg1	infiltration					1157:1168	γδ T cell infiltration	1147:1168	γδ T cell infiltration	1147:1168	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	6	6	theme	γδ	1147:1148	arg1	infiltration					1157:1168	γδ T cell infiltration	1147:1168	γδ T cell infiltration	1147:1168	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	3	7	theme	autoimmune	408:417	arg1	development					427:437	autoimmune disease development	408:437	autoimmune disease development	408:437	Accumulating evidence has demonstrated that protein glycosylation is substantially altered in autoimmune disease development, but the mechanisms by which glycans trigger these autoreactive immune responses are still largely unclear.
36921032	7	8	theme	mannose-γδ	1233:1242	arg1	axis					1258:1261	a mannose-γδ T cell-IL-17a axis	1231:1261	a mannose-γδ T cell-IL-17a axis	1231:1261	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	2	9	theme	self-tolerance	298:311	arg1	mechanisms					284:293	mechanisms	284:293	mechanisms of self-tolerance	284:311	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	5	10	theme	increased	890:898	arg1	infiltration					910:921	increased γδ T cell infiltration	890:921	increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice	890:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	4	11	from	surface	635:641	arg1	presence					576:583	presence	576:583	presence of microbial-associated mannose structures at the surface of the kidney	576:655	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	4	12	theme	-mediated	762:770	arg1	response					783:790	a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response	725:790	a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response	725:790	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	0	13	link	Host-derived	0:11	arg1	glycans					21:27	Host-derived mannose glycans	0:27	Host-derived mannose glycans	0:27	Host-derived mannose glycans trigger a pathogenic γδ T cell/IL-17a axis in autoimmunity.
36921032	5	14	theme	T	903:903	arg1	infiltration					910:921	increased γδ T cell infiltration	890:921	increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice	890:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	5	15	theme	mannose	846:852	arg1	structures					854:863	mannose structures	846:863	mannose structures	846:863	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	5	16	theme	structures	854:863	arg1	abundance					833:841	a higher abundance	824:841	a higher abundance of mannose structures	824:863	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	6	17	theme	branched	1092:1099	arg1	N-glycans					1101:1109	tolerogenic branched N-glycans	1080:1109	tolerogenic branched N-glycans	1080:1109	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	3	18	theme	autoreactive	490:501	arg1	responses					510:518	these autoreactive immune responses	484:518	these autoreactive immune responses	484:518	Accumulating evidence has demonstrated that protein glycosylation is substantially altered in autoimmune disease development, but the mechanisms by which glycans trigger these autoreactive immune responses are still largely unclear.
36921032	0	19	theme	mannose	13:19	arg1	glycans					21:27	Host-derived mannose glycans	0:27	Host-derived mannose glycans	0:27	Host-derived mannose glycans trigger a pathogenic γδ T cell/IL-17a axis in autoimmunity.
36921032	7	20	theme	disease	1374:1380	arg1	treatment					1382:1390	autoimmune disease treatment	1363:1390	autoimmune disease treatment	1363:1390	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	5	21	from	development	976:986	arg1	mice					1006:1009	older mice	1000:1009	older mice	1000:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	4	22	theme	T	707:707	arg1	cells					709:713	DC-SIGN-expressing γδ T cells	685:713	DC-SIGN-expressing γδ T cells	685:713	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	0	23	theme	Host-derived	0:11	arg1	glycans					21:27	Host-derived mannose glycans	0:27	Host-derived mannose glycans	0:27	Host-derived mannose glycans trigger a pathogenic γδ T cell/IL-17a axis in autoimmunity.
36921032	4	24	theme	mannose	609:615	arg1	structures					617:626	microbial-associated mannose structures	588:626	microbial-associated mannose structures	588:626	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	1	25	theme	Autoimmune	89:98	arg1	diseases					100:107	Autoimmune diseases	89:107	Autoimmune diseases	89:107	Autoimmune diseases are life-threatening disorders that cause increasing disability over time.
36921032	1	25	theme	Autoimmune	89:98	arg1	disorders					130:138	life-threatening disorders	113:138	life-threatening disorders that cause increasing disability over time	113:181	Autoimmune diseases are life-threatening disorders that cause increasing disability over time.
36921032	3	26	theme	disease	419:425	arg1	development					427:437	autoimmune disease development	408:437	autoimmune disease development	408:437	Accumulating evidence has demonstrated that protein glycosylation is substantially altered in autoimmune disease development, but the mechanisms by which glycans trigger these autoreactive immune responses are still largely unclear.
36921032	4	27	theme	microbial-associated	588:607	arg1	structures					617:626	microbial-associated mannose structures	588:626	microbial-associated mannose structures	588:626	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	4	28	attach	presence	576:583	arg2	structures					617:626	microbial-associated mannose structures	588:626	microbial-associated mannose structures	588:626	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	4	28	attach	presence	576:583	arg1	surface					635:641	the surface	631:641	the surface of the kidney	631:655	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	4	29	theme	DC-SIGN-expressing	685:702	arg1	cells					709:713	DC-SIGN-expressing γδ T cells	685:713	DC-SIGN-expressing γδ T cells	685:713	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	5	30	theme	lupus	991:995	arg1	development					976:986	spontaneous development	964:986	spontaneous development of lupus in older mice	964:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	5	31	theme	older	1000:1004	arg1	mice					1006:1009	older mice	1000:1009	older mice	1000:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	5	32	theme	cell	905:908	arg1	infiltration					910:921	increased γδ T cell infiltration	890:921	increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice	890:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	0	33	theme	pathogenic	39:48	arg1	axis					67:70	a pathogenic γδ T cell/IL-17a axis	37:70	a pathogenic γδ T cell/IL-17a axis	37:70	Host-derived mannose glycans trigger a pathogenic γδ T cell/IL-17a axis in autoimmunity.
36921032	4	34	theme	structures	617:626	arg1	presence					576:583	presence	576:583	presence of microbial-associated mannose structures at the surface of the kidney	576:655	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	2	35	theme	lupus	193:197	arg1	SLE					214:216	SLE	214:216	SLE	214:216	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	2	35	theme	lupus	193:197	arg1	erythematosus					199:211	Systemic lupus erythematosus	184:211	Systemic lupus erythematosus (SLE)	184:217	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	3	36	theme	immune	503:508	arg1	responses					510:518	these autoreactive immune responses	484:518	these autoreactive immune responses	484:518	Accumulating evidence has demonstrated that protein glycosylation is substantially altered in autoimmune disease development, but the mechanisms by which glycans trigger these autoreactive immune responses are still largely unclear.
36921032	2	37	theme	Systemic	184:191	arg1	SLE					214:216	SLE	214:216	SLE	214:216	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	2	37	theme	Systemic	184:191	arg1	erythematosus					199:211	Systemic lupus erythematosus	184:211	Systemic lupus erythematosus (SLE)	184:217	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	7	38	theme	cell-IL-17a	1246:1256	arg1	axis					1258:1261	a mannose-γδ T cell-IL-17a axis	1231:1261	a mannose-γδ T cell-IL-17a axis	1231:1261	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	4	39	theme	γδ	704:705	arg1	cells					709:713	DC-SIGN-expressing γδ T cells	685:713	DC-SIGN-expressing γδ T cells	685:713	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	0	40	theme	T	53:53	arg1	axis					67:70	a pathogenic γδ T cell/IL-17a axis	37:70	a pathogenic γδ T cell/IL-17a axis	37:70	Host-derived mannose glycans trigger a pathogenic γδ T cell/IL-17a axis in autoimmunity.
36921032	6	41	theme	N-acetylglucosamine	1012:1030	arg1	supplementation					1032:1046	N-acetylglucosamine supplementation	1012:1046	N-acetylglucosamine supplementation	1012:1046	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	2	42	theme	autoimmune	229:238	arg1	diseases					240:247	other autoimmune diseases	223:247	other autoimmune diseases	223:247	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	1	43	theme	life-threatening	113:128	arg1	diseases					100:107	Autoimmune diseases	89:107	Autoimmune diseases	89:107	Autoimmune diseases are life-threatening disorders that cause increasing disability over time.
36921032	1	43	theme	life-threatening	113:128	arg1	disorders					130:138	life-threatening disorders	113:138	life-threatening disorders that cause increasing disability over time	113:181	Autoimmune diseases are life-threatening disorders that cause increasing disability over time.
36921032	0	44	theme	γδ	50:51	arg1	axis					67:70	a pathogenic γδ T cell/IL-17a axis	37:70	a pathogenic γδ T cell/IL-17a axis	37:70	Host-derived mannose glycans trigger a pathogenic γδ T cell/IL-17a axis in autoimmunity.
36921032	3	45	gly	glycosylation	366:378	arg1	development					427:437	autoimmune disease development	408:437	autoimmune disease development	408:437	Accumulating evidence has demonstrated that protein glycosylation is substantially altered in autoimmune disease development, but the mechanisms by which glycans trigger these autoreactive immune responses are still largely unclear.
36921032	2	46	theme	other	223:227	arg1	diseases					240:247	other autoimmune diseases	223:247	other autoimmune diseases	223:247	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	6	47	theme	disease	1182:1188	arg1	development					1190:1200	disease development	1182:1200	disease development	1182:1200	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	3	48	theme	Accumulating	314:325	arg1	evidence					327:334	Accumulating evidence	314:334	Accumulating evidence	314:334	Accumulating evidence has demonstrated that protein glycosylation is substantially altered in autoimmune disease development, but the mechanisms by which glycans trigger these autoreactive immune responses are still largely unclear.
36921032	5	49	theme	spontaneous	964:974	arg1	development					976:986	spontaneous development	964:986	spontaneous development of lupus in older mice	964:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	6	50	theme	N-glycans	1101:1109	arg1	biosynthesis					1064:1075	biosynthesis	1064:1075	biosynthesis of tolerogenic branched N-glycans	1064:1109	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	4	51	theme	autoimmune	772:781	arg1	response					783:790	a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response	725:790	a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response	725:790	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	4	52	theme	kidney	650:655	arg1	surface					635:641	the surface	631:641	the surface of the kidney	631:655	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	5	53	theme	γδ	900:901	arg1	infiltration					910:921	increased γδ T cell infiltration	890:921	increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice	890:1009	Mice lacking Mgat5, which have a higher abundance of mannose structures in the kidney, displayed increased γδ T cell infiltration into the kidney that was associated with spontaneous development of lupus in older mice.
36921032	2	54	theme	immune	260:265	arg1	stimuli					267:273	immune stimuli	260:273	immune stimuli	260:273	Systemic lupus erythematosus (SLE) and other autoimmune diseases arise when immune stimuli override mechanisms of self-tolerance.
36921032	4	55	theme	cells	709:713	arg1	recognition					670:680	the recognition	666:680	the recognition of DC-SIGN-expressing γδ T cells	666:713	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	6	56	theme	tolerogenic	1080:1090	arg1	N-glycans					1101:1109	tolerogenic branched N-glycans	1080:1109	tolerogenic branched N-glycans	1080:1109	N-acetylglucosamine supplementation, which promoted biosynthesis of tolerogenic branched N-glycans in the kidney, was found to inhibit γδ T cell infiltration and control disease development.
36921032	0	57	theme	cell/IL-17a	55:65	arg1	axis					67:70	a pathogenic γδ T cell/IL-17a axis	37:70	a pathogenic γδ T cell/IL-17a axis	37:70	Host-derived mannose glycans trigger a pathogenic γδ T cell/IL-17a axis in autoimmunity.
36921032	7	58	theme	T	1244:1244	arg1	axis					1258:1261	a mannose-γδ T cell-IL-17a axis	1231:1261	a mannose-γδ T cell-IL-17a axis	1231:1261	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	7	59	theme	autoimmune	1363:1372	arg1	treatment					1382:1390	autoimmune disease treatment	1363:1390	autoimmune disease treatment	1363:1390	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	7	60	theme	therapeutic	1338:1348	arg1	strategy					1350:1357	a therapeutic strategy	1336:1357	a therapeutic strategy for autoimmune disease treatment	1336:1390	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	7	60	theme	therapeutic	1338:1348	arg1	reprogramming					1319:1331	glycometabolic reprogramming	1304:1331	glycometabolic reprogramming	1304:1331	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	4	61	theme	pathogenic	727:736	arg1	response					783:790	a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response	725:790	a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response	725:790	In this study, we found that presence of microbial-associated mannose structures at the surface of the kidney triggers the recognition of DC-SIGN-expressing γδ T cells, inducing a pathogenic interleukin-17a (IL-17a)-mediated autoimmune response.
36921032	7	62	theme	glycometabolic	1304:1317	arg1	strategy					1350:1357	a therapeutic strategy	1336:1357	a therapeutic strategy for autoimmune disease treatment	1336:1390	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
36921032	7	62	theme	glycometabolic	1304:1317	arg1	reprogramming					1319:1331	glycometabolic reprogramming	1304:1331	glycometabolic reprogramming	1304:1331	Together, this work reveals a mannose-γδ T cell-IL-17a axis in SLE immunopathogenesis and highlights glycometabolic reprogramming as a therapeutic strategy for autoimmune disease treatment.
37047593	2	0	theme	GO	530:531	arg1	concentrations					512:525	two different concentrations	498:525	two different concentrations of GO	498:531	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	3	1	theme	markers	729:735	arg1	expression					702:711	the protein expression	690:711	the protein expression of inflammatory markers	690:735	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	3	1	theme	markers	729:735	arg1	gene					681:684	The gene	677:684	The gene	677:684	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	2	2	theme	p65/NLRP3	597:605	arg1	modulation					541:550	the modulation	537:550	the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	537:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	4	3	theme	polypropylene	922:934	arg1	threads					943:949	the polypropylene suture threads	918:949	the polypropylene suture threads buttons-GO constructs (PPSTBs-GO)	918:983	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	3	4	theme	western	836:842	arg1	blotting					844:851	western blotting	836:851	western blotting	836:851	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	6	5	theme	inflammatory	1299:1310	arg1	process					1312:1318	the inflammatory process	1295:1318	the inflammatory process	1295:1318	Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway.
37047593	5	6	theme	electron	1183:1190	arg1	SEM					1204:1206	SEM	1204:1206	SEM	1204:1206	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	5	6	theme	electron	1183:1190	arg1	microscopy					1192:1201	scanning electron microscopy	1174:1201	scanning electron microscopy (SEM)	1174:1207	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	1	7	theme	thermal	264:270	arg1	conductivity					284:295	thermal or electric conductivity	264:295	thermal or electric conductivity	264:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	2	8	theme	coli	634:637	arg1	LPS-E					669:673	LPS-E	669:673	LPS-E	669:673	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	8	theme	coli	634:637	arg1	lipopolysaccharide					649:666	the Escherichia coli (E. coli) lipopolysaccharide	618:666	the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	618:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	3	9	theme	real-time	821:829	arg1	PCR					831:833	real-time PCR	821:833	real-time PCR	821:833	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	0	10	theme	Escherichia	106:116	arg1	Lipopolysaccharide					123:140	Escherichia coli Lipopolysaccharide	106:140	Escherichia coli Lipopolysaccharide	106:140	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	4	11	theme	inflammatory	1013:1024	arg1	markers					1026:1032	inflammatory markers	1013:1032	inflammatory markers	1013:1032	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	1	12	theme	electric	275:282	arg1	conductivity					284:295	thermal or electric conductivity	264:295	thermal or electric conductivity	264:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	3	13	theme	gingival	790:797	arg1	hGFs					812:815	hGFs	812:815	hGFs	812:815	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	3	13	theme	gingival	790:797	arg1	fibroblasts					799:809	primary human gingival fibroblasts	776:809	primary human gingival fibroblasts (hGFs)	776:816	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	4	14	dep	concentrations	895:908	arg1	GO					892:893	GO	892:893	GO	892:893	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	2	15	theme	polypropylene	437:449	arg1	PPSTBs					475:480	PPSTBs	475:480	PPSTBs	475:480	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	15	theme	polypropylene	437:449	arg1	buttons					466:472	polypropylene suture threads buttons	437:472	polypropylene suture threads buttons (PPSTBs)	437:481	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	5	16	dep	hGFs	1066:1069	arg1	morphology					1071:1080	morphology	1071:1080	morphology	1071:1080	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	5	16	dep	hGFs	1066:1069	arg1	hGFs					1066:1069	The hGFs morphology and adhesion	1062:1093	The hGFs morphology and adhesion on the PPSTBs-GO constructs	1062:1121	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	5	16	dep	hGFs	1066:1069	arg1	adhesion					1086:1093	adhesion	1086:1093	adhesion	1086:1093	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	4	17	theme	markers	1026:1032	arg1	expression					999:1008	the expression	995:1008	the expression of inflammatory markers in hGFs treated with LPS-E	995:1059	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	0	18	dep	Escherichia	106:116	arg1	coli					118:121	coli	118:121	coli	118:121	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	4	19	theme	buttons-GO	951:960	arg1	PPSTBs-GO					974:982	PPSTBs-GO	974:982	PPSTBs-GO	974:982	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	4	19	theme	buttons-GO	951:960	arg1	constructs					962:971	buttons-GO constructs	951:971	the polypropylene suture threads buttons-GO constructs (PPSTBs-GO)	918:983	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	3	20	theme	primary	776:782	arg1	hGFs					812:815	hGFs	812:815	hGFs	812:815	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	3	20	theme	primary	776:782	arg1	fibroblasts					799:809	primary human gingival fibroblasts	776:809	primary human gingival fibroblasts (hGFs)	776:816	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	0	21	theme	Graphene	9:16	arg1	Buttons					53:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	1	22	attach	derived	164:170	arg1	graphene					177:184	graphene	177:184	graphene	177:184	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	22	attach	derived	164:170	arg2	oxide					152:156	Graphene oxide	143:156	Graphene oxide (GO)	143:161	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	6	23	theme	88/NFκB	1337:1343	arg1	pathway					1355:1361	TLR4/MyD 88/NFκB p65/NLRP3 pathway	1328:1361	TLR4/MyD 88/NFκB p65/NLRP3 pathway	1328:1361	Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway.
37047593	0	24	theme	Enriched	0:7	arg1	Buttons					53:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	5	25	theme	light	1156:1160	arg1	microscopy					1162:1171	inverted light microscopy	1147:1171	inverted light microscopy	1147:1171	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	1	26	theme	remarkable	191:200	arg1	strength					250:257	strength	250:257	strength	250:257	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	26	theme	remarkable	191:200	arg1	properties					220:229	remarkable chemical-physical properties	191:229	remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity	191:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	26	theme	remarkable	191:200	arg1	stability					239:247	stability	239:247	stability	239:247	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	26	theme	remarkable	191:200	arg1	conductivity					284:295	thermal or electric conductivity	264:295	thermal or electric conductivity	264:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	0	27	theme	Suture	38:43	arg1	Buttons					53:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	2	28	theme	88/NFκB	589:595	arg1	p65/NLRP3					597:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3	555:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	555:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	5	29	from	hGFs	1066:1069	arg1	constructs					1112:1121	the PPSTBs-GO constructs	1098:1121	the PPSTBs-GO constructs	1098:1121	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	1	30	theme	chemical-physical	202:218	arg1	strength					250:257	strength	250:257	strength	250:257	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	30	theme	chemical-physical	202:218	arg1	properties					220:229	remarkable chemical-physical properties	191:229	remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity	191:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	30	theme	chemical-physical	202:218	arg1	stability					239:247	stability	239:247	stability	239:247	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	30	theme	chemical-physical	202:218	arg1	conductivity					284:295	thermal or electric conductivity	264:295	thermal or electric conductivity	264:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	0	31	theme	Oxide-Polypropylene	18:36	arg1	Buttons					53:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	3	32	theme	in	758:759	arg1	model					767:771	an in vitro model	755:771	an in vitro model of primary human gingival fibroblasts (hGFs)	755:816	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	2	33	theme	TLR4/MyD	580:587	arg1	p65/NLRP3					597:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3	555:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	555:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	34	theme	buttons	466:472	arg1	effects					426:432	the anti-inflammatory effects	404:432	the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	404:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	35	dep	coli	634:637	arg1	coli					643:646	E. coli	640:646	E. coli	640:646	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	36	theme	pathway	572:578	arg1	p65/NLRP3					597:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3	555:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	555:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	1	37	contain	has	187:189	arg2	stability					239:247	stability	239:247	stability	239:247	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	37	contain	has	187:189	arg2	properties					220:229	remarkable chemical-physical properties	191:229	remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity	191:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	37	contain	has	187:189	arg1	oxide					152:156	Graphene oxide	143:156	Graphene oxide (GO)	143:161	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	37	contain	has	187:189	arg2	conductivity					284:295	thermal or electric conductivity	264:295	thermal or electric conductivity	264:295	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	1	37	contain	has	187:189	arg2	strength					250:257	strength	250:257	strength	250:257	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	2	38	theme	anti-inflammatory	408:424	arg1	effects					426:432	the anti-inflammatory effects	404:432	the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	404:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	39	theme	threads	458:464	arg1	PPSTBs					475:480	PPSTBs	475:480	PPSTBs	475:480	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	39	theme	threads	458:464	arg1	buttons					466:472	polypropylene suture threads buttons	437:472	polypropylene suture threads buttons (PPSTBs)	437:481	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	0	40	theme	Threads	45:51	arg1	Buttons					53:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons	0:59	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	1	41	theme	antibacterial	320:332	arg1	properties					356:365	antibacterial and anti-inflammatory properties	320:365	antibacterial and anti-inflammatory properties	320:365	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	2	42	theme	different	502:510	arg1	concentrations					512:525	two different concentrations	498:525	two different concentrations of GO	498:531	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	5	43	theme	real-time	1214:1222	arg1	PCR					1224:1226	real-time PCR	1214:1226	real-time PCR	1214:1226	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	2	44	from	effects	426:432	arg1	modulation					541:550	the modulation	537:550	the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	537:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	45	theme	suture	451:456	arg1	PPSTBs					475:480	PPSTBs	475:480	PPSTBs	475:480	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	2	45	theme	suture	451:456	arg1	buttons					466:472	polypropylene suture threads buttons	437:472	polypropylene suture threads buttons (PPSTBs)	437:481	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	3	46	theme	fibroblasts	799:809	arg1	model					767:771	an in vitro model	755:771	an in vitro model of primary human gingival fibroblasts (hGFs)	755:816	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	6	47	theme	p65/NLRP3	1345:1353	arg1	pathway					1355:1361	TLR4/MyD 88/NFκB p65/NLRP3 pathway	1328:1361	TLR4/MyD 88/NFκB p65/NLRP3 pathway	1328:1361	Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway.
37047593	5	48	theme	PPSTBs-GO	1102:1110	arg1	constructs					1112:1121	the PPSTBs-GO constructs	1098:1121	the PPSTBs-GO constructs	1098:1121	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	1	49	theme	Graphene	143:150	arg1	oxide					152:156	Graphene oxide	143:156	Graphene oxide (GO)	143:161	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	2	50	theme	inflammatory	559:570	arg1	p65/NLRP3					597:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3	555:605	the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E)	555:674	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	1	51	theme	anti-inflammatory	338:354	arg1	properties					356:365	antibacterial and anti-inflammatory properties	320:365	antibacterial and anti-inflammatory properties	320:365	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	3	52	dep	in	758:759	arg1	vitro					761:765	vitro	761:765	vitro	761:765	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	6	53	theme	enriched	1266:1273	arg1	PPSTBs-GO					1275:1283	enriched PPSTBs-GO	1266:1283	enriched PPSTBs-GO	1266:1283	Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway.
37047593	2	54	theme	present	372:378	arg1	study					380:384	The present study	368:384	The present study	368:384	The present study aimed to evaluate the anti-inflammatory effects of polypropylene suture threads buttons (PPSTBs), enriched with two different concentrations of GO, in the modulation of the inflammatory pathway TLR4/MyD 88/NFκB p65/NLRP3 induced by the Escherichia coli (E. coli) lipopolysaccharide (LPS-E).
37047593	3	55	theme	human	784:788	arg1	hGFs					812:815	hGFs	812:815	hGFs	812:815	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	3	55	theme	human	784:788	arg1	fibroblasts					799:809	primary human gingival fibroblasts	776:809	primary human gingival fibroblasts (hGFs)	776:816	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	6	56	theme	TLR4/MyD	1328:1335	arg1	pathway					1355:1361	TLR4/MyD 88/NFκB p65/NLRP3 pathway	1328:1361	TLR4/MyD 88/NFκB p65/NLRP3 pathway	1328:1361	Together, these results suggest that enriched PPSTBs-GO modulates the inflammatory process through TLR4/MyD 88/NFκB p65/NLRP3 pathway.
37047593	3	57	theme	protein	694:700	arg1	expression					702:711	the protein expression	690:711	the protein expression of inflammatory markers	690:735	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	3	58	theme	immunofluorescence	858:875	arg1	analysis					877:884	immunofluorescence analysis	858:884	immunofluorescence analysis	858:884	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	1	59	dep	oxide	152:156	arg1	GO					159:160	GO	159:160	GO	159:160	Graphene oxide (GO), derived from graphene, has remarkable chemical-physical properties such as stability, strength, and thermal or electric conductivity and additionally shows antibacterial and anti-inflammatory properties.
37047593	4	60	from	expression	999:1008	arg1	hGFs					1037:1040	hGFs	1037:1040	hGFs treated with LPS-E	1037:1059	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	5	61	theme	scanning	1174:1181	arg1	SEM					1204:1206	SEM	1204:1206	SEM	1204:1206	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	5	61	theme	scanning	1174:1181	arg1	microscopy					1192:1201	scanning electron microscopy	1174:1201	scanning electron microscopy (SEM)	1174:1207	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	4	62	theme	suture	936:941	arg1	threads					943:949	the polypropylene suture threads	918:949	the polypropylene suture threads buttons-GO constructs (PPSTBs-GO)	918:983	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	5	63	theme	inverted	1147:1154	arg1	microscopy					1162:1171	inverted light microscopy	1147:1171	inverted light microscopy	1147:1171	The hGFs morphology and adhesion on the PPSTBs-GO constructs were also visualized by inverted light microscopy, scanning electron microscopy (SEM), and real-time PCR.
37047593	0	64	theme	Inflammatory	74:85	arg1	Pathway					87:93	the Inflammatory Pathway	70:93	the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide	70:140	Enriched Graphene Oxide-Polypropylene Suture Threads Buttons Modulate the Inflammatory Pathway Induced by Escherichia coli Lipopolysaccharide.
37047593	3	65	theme	inflammatory	716:727	arg1	markers					729:735	inflammatory markers	716:735	inflammatory markers	716:735	The gene and the protein expression of inflammatory markers were evaluated in an in vitro model of primary human gingival fibroblasts (hGFs) by real-time PCR, western blotting, and immunofluorescence analysis.
37047593	4	66	dep	threads	943:949	arg1	PPSTBs-GO					974:982	PPSTBs-GO	974:982	PPSTBs-GO	974:982	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
37047593	4	66	dep	threads	943:949	arg1	constructs					962:971	buttons-GO constructs	951:971	the polypropylene suture threads buttons-GO constructs (PPSTBs-GO)	918:983	Both GO concentrations used in the polypropylene suture threads buttons-GO constructs (PPSTBs-GO) decreased the expression of inflammatory markers in hGFs treated with LPS-E.
36975700	1	0	theme	biochemical	222:232	arg1	composition					234:244	biochemical composition	222:244	biochemical composition	222:244	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	12	1	theme	cycling	1892:1898	arg1	processes					1872:1880	the large-scale processes	1856:1880	the large-scale processes of carbon cycling and sedimentation	1856:1916	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	2	2	theme	exopolymer	595:604	arg1	particles					606:614	transparent exopolymer particles	583:614	transparent exopolymer particles (TEP)	583:620	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	2	theme	exopolymer	595:604	arg1	TEP					617:619	TEP	617:619	TEP	617:619	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	12	3	theme	sedimentation	1904:1916	arg1	processes					1872:1880	the large-scale processes	1856:1880	the large-scale processes of carbon cycling and sedimentation	1856:1916	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	2	4	theme	Coomassie	626:634	arg1	CSP					657:659	CSP	657:659	CSP	657:659	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	4	theme	Coomassie	626:634	arg1	particles					646:654	Coomassie stainable particles	626:654	Coomassie stainable particles (CSP)	626:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	11	5	contain	contained	1674:1682	arg2	eDNA					1684:1687	eDNA	1684:1687	eDNA	1684:1687	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	11	5	contain	contained	1674:1682	arg1	EPS					1627:1629	bacterial EPS	1617:1629	bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA	1617:1687	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	12	6	theme	large-scale	1860:1870	arg1	processes					1872:1880	the large-scale processes	1856:1880	the large-scale processes of carbon cycling and sedimentation	1856:1916	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	2	7	theme	proteins	571:578	arg1	identification					526:539	the identification	522:539	the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP)	522:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	5	8	with	filters	923:929	arg1	filtration					950:959	gentle vacuum filtration	936:959	gentle vacuum filtration	936:959	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	2	9	theme	dynamic	288:294	arg1	interactions					307:318	dynamic ecological interactions	288:318	dynamic ecological interactions between marine microorganisms and MGPs	288:357	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	10	theme	acidic	544:549	arg1	polysaccharides					551:565	acidic polysaccharides	544:565	acidic polysaccharides	544:565	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	12	11	theme	MGPs	1838:1841	arg1	dynamics					1817:1824	micro-scale dynamics	1805:1824	micro-scale dynamics	1805:1824	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	12	11	theme	MGPs	1838:1841	arg1	fate					1830:1833	fate	1830:1833	fate	1830:1833	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	5	12	theme	sterile	1041:1047	arg1	seawater					1049:1056	sterile seawater	1041:1056	sterile seawater	1041:1056	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	1	13	from	ubiquitous	186:195	arg1	oceans					204:209	the oceans	200:209	the oceans	200:209	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	9	14	theme	laser	1383:1387	arg1	CLSM					1410:1413	CLSM	1410:1413	CLSM	1410:1413	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	9	14	theme	laser	1383:1387	arg1	microscopy					1398:1407	Confocal laser scanning microscopy	1374:1407	Confocal laser scanning microscopy (CLSM)	1374:1414	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	2	15	from	particles	646:654	arg1	identification					526:539	the identification	522:539	the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP)	522:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	4	16	theme	Sea	855:857	arg1	seawater					867:874	North Sea surface seawater	849:874	North Sea surface seawater	849:874	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	8	17	theme	cells	1367:1371	arg1	staining					1355:1362	the live/dead staining	1341:1362	the live/dead staining of cells	1341:1371	TOTO-3 was also used to stain eDNA, with ConA to localise glycoproteins and SYTO-9 for the live/dead staining of cells.
36975700	11	18	theme	Pseudoalteromonas	1636:1652	arg1	atlantica					1654:1662	Pseudoalteromonas atlantica	1636:1662	Pseudoalteromonas atlantica	1636:1662	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	1	19	from	oceans	204:209	arg1	ubiquitous					186:195	ubiquitous	186:195	ubiquitous	186:195	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	5	20	theme	polycarbonate	904:916	arg1	filters					923:929	polycarbonate (PC) filters	904:929	polycarbonate (PC) filters with gentle vacuum filtration	904:959	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	0	21	theme	DNA	60:62	arg1	Presence					34:41	the Presence	30:41	the Presence of Extracellular DNA in Marine Gel Particles	30:86	Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles.
36975700	1	22	theme	amorphous	120:128	arg1	particles					100:108	Marine gel particles	89:108	Marine gel particles (MGP)	89:114	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	22	theme	amorphous	120:128	arg1	exudates					139:146	amorphous hydrogel exudates	120:146	amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans	120:209	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	11	23	from	atlantica	1654:1662	arg1	EPS					1627:1629	bacterial EPS	1617:1629	bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA	1617:1687	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	0	24	theme	Marine	67:72	arg1	Particles					78:86	Marine Gel Particles	67:86	Marine Gel Particles	67:86	Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles.
36975700	12	25	theme	micro-scale	1805:1815	arg1	dynamics					1817:1824	micro-scale dynamics	1805:1824	micro-scale dynamics	1805:1824	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	4	26	from	seawater	764:771	arg1	way					737:739	a new way	731:739	a new way of isolating MGPs from seawater	731:771	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	2	27	theme	transparent	583:593	arg1	particles					606:614	transparent exopolymer particles	583:614	transparent exopolymer particles (TEP)	583:620	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	27	theme	transparent	583:593	arg1	TEP					617:619	TEP	617:619	TEP	617:619	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	4	28	theme	MGPs	754:757	arg1	way					737:739	a new way	731:739	a new way of isolating MGPs from seawater	731:771	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	7	29	theme	fluorescent	1146:1156	arg1	microscopy					1158:1167	fluorescent microscopy	1146:1167	fluorescent microscopy using YOYO-1 (for eDNA)	1146:1191	eDNA was detected by fluorescent microscopy using YOYO-1 (for eDNA), with Nile red (targeting cell membranes) as a counterstain.
36975700	5	30	theme	gentle	936:941	arg1	filtration					950:959	gentle vacuum filtration	936:959	gentle vacuum filtration	936:959	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	2	31	theme	stainable	636:644	arg1	CSP					657:659	CSP	657:659	CSP	657:659	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	31	theme	stainable	636:644	arg1	particles					646:654	Coomassie stainable particles	626:654	Coomassie stainable particles (CSP)	626:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	4	32	from	DNA	835:837	arg1	seawater					867:874	North Sea surface seawater	849:874	North Sea surface seawater	849:874	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	9	33	theme	polysaccharides	1454:1468	arg1	presence					1429:1436	the presence	1425:1436	the presence of proteins and polysaccharides	1425:1468	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	4	34	theme	new	733:735	arg1	way					737:739	a new way	731:739	a new way of isolating MGPs from seawater	731:771	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	5	35	theme	PC	919:920	arg1	filters					923:929	polycarbonate (PC) filters	904:929	polycarbonate (PC) filters with gentle vacuum filtration	904:959	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	2	36	theme	substances	435:444	arg1	secretion					377:385	secretion	377:385	secretion	377:385	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	36	theme	substances	435:444	arg1	mixing					391:396	mixing	391:396	mixing	391:396	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	37	theme	extracellular	411:423	arg1	acids					468:472	nucleic acids	460:472	nucleic acids	460:472	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	37	theme	extracellular	411:423	arg1	EPS					447:449	EPS	447:449	EPS	447:449	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	37	theme	extracellular	411:423	arg1	substances					435:444	bacterial extracellular polymeric substances	401:444	bacterial extracellular polymeric substances (EPS) such as nucleic acids	401:472	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	1	38	theme	gel	96:98	arg1	MGP					111:113	MGP	111:113	MGP	111:113	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	38	theme	gel	96:98	arg1	particles					100:108	Marine gel particles	89:108	Marine gel particles (MGP)	89:114	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	38	theme	gel	96:98	arg1	exudates					139:146	amorphous hydrogel exudates	120:146	amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans	120:209	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	2	39	theme	nucleic	460:466	arg1	acids					468:472	nucleic acids	460:472	nucleic acids	460:472	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	40	from	particles	606:614	arg1	identification					526:539	the identification	522:539	the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP)	522:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	11	41	theme	experimental	1587:1598	arg1	system					1604:1609	a model experimental MGP system	1579:1609	a model experimental MGP system	1579:1609	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	7	42	theme	cell	1219:1222	arg1	membranes					1224:1232	cell membranes	1219:1232	cell membranes	1219:1232	eDNA was detected by fluorescent microscopy using YOYO-1 (for eDNA), with Nile red (targeting cell membranes) as a counterstain.
36975700	12	43	theme	eDNA	1740:1743	arg1	occurrence					1726:1735	the occurrence	1722:1735	the occurrence of eDNA in MGPs	1722:1751	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	12	44	from	occurrence	1726:1735	arg1	MGPs					1748:1751	MGPs	1748:1751	MGPs	1748:1751	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	11	45	theme	bacterial	1617:1625	arg1	EPS					1627:1629	bacterial EPS	1617:1629	bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA	1617:1687	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	4	46	theme	liquid	776:781	arg1	suspension					783:792	liquid suspension	776:792	liquid suspension	776:792	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	6	47	theme	resulting	1063:1071	arg1	MGPs					1073:1076	The resulting MGPs	1059:1076	The resulting MGPs	1059:1076	The resulting MGPs ranged in size from 0.4 to 100 µm in diameter.
36975700	8	48	gly	glycoproteins	1312:1324	arg1	glycoproteins					1312:1324	glycoproteins	1312:1324	glycoproteins	1312:1324	TOTO-3 was also used to stain eDNA, with ConA to localise glycoproteins and SYTO-9 for the live/dead staining of cells.
36975700	2	49	from	polysaccharides	551:565	arg1	particles					606:614	transparent exopolymer particles	583:614	transparent exopolymer particles (TEP)	583:620	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	49	from	polysaccharides	551:565	arg1	CSP					657:659	CSP	657:659	CSP	657:659	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	49	from	polysaccharides	551:565	arg1	particles					646:654	Coomassie stainable particles	626:654	Coomassie stainable particles (CSP)	626:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	49	from	polysaccharides	551:565	arg1	TEP					617:619	TEP	617:619	TEP	617:619	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	12	50	theme	carbon	1885:1890	arg1	cycling					1892:1898	carbon cycling	1885:1898	carbon cycling	1885:1898	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	4	51	theme	North	849:853	arg1	seawater					867:874	North Sea surface seawater	849:874	North Sea surface seawater	849:874	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	2	52	from	proteins	571:578	arg1	particles					606:614	transparent exopolymer particles	583:614	transparent exopolymer particles (TEP)	583:620	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	52	from	proteins	571:578	arg1	CSP					657:659	CSP	657:659	CSP	657:659	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	52	from	proteins	571:578	arg1	particles					646:654	Coomassie stainable particles	626:654	Coomassie stainable particles (CSP)	626:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	52	from	proteins	571:578	arg1	TEP					617:619	TEP	617:619	TEP	617:619	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	4	53	theme	extracellular	821:833	arg1	eDNA					840:843	eDNA	840:843	eDNA	840:843	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	4	53	theme	extracellular	821:833	arg1	DNA					835:837	extracellular DNA	821:837	extracellular DNA (eDNA) in North Sea surface seawater	821:874	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	11	54	theme	eDNA	1558:1561	arg1	role					1550:1553	the role	1546:1553	the role of eDNA	1546:1561	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	2	55	theme	ecological	296:305	arg1	interactions					307:318	dynamic ecological interactions	288:318	dynamic ecological interactions between marine microorganisms and MGPs	288:357	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	6	56	dep	100	1105:1107	arg1	to					1102:1103	to	1102:1103	to	1102:1103	The resulting MGPs ranged in size from 0.4 to 100 µm in diameter.
36975700	8	57	theme	live/dead	1345:1353	arg1	staining					1355:1362	the live/dead staining	1341:1362	the live/dead staining of cells	1341:1371	TOTO-3 was also used to stain eDNA, with ConA to localise glycoproteins and SYTO-9 for the live/dead staining of cells.
36975700	9	58	theme	Confocal	1374:1381	arg1	CLSM					1410:1413	CLSM	1410:1413	CLSM	1410:1413	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	9	58	theme	Confocal	1374:1381	arg1	microscopy					1398:1407	Confocal laser scanning microscopy	1374:1407	Confocal laser scanning microscopy (CLSM)	1374:1414	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	12	59	dep	dynamics	1817:1824	arg1	the					1801:1803	the	1801:1803	the	1801:1803	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	5	60	theme	seawater	1049:1056	arg1	volume					1031:1036	a smaller volume	1021:1036	a smaller volume of sterile seawater	1021:1056	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	9	61	theme	scanning	1389:1396	arg1	CLSM					1410:1413	CLSM	1410:1413	CLSM	1410:1413	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	9	61	theme	scanning	1389:1396	arg1	microscopy					1398:1407	Confocal laser scanning microscopy	1374:1407	Confocal laser scanning microscopy (CLSM)	1374:1414	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	4	62	theme	surface	859:865	arg1	seawater					867:874	North Sea surface seawater	849:874	North Sea surface seawater	849:874	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	2	63	theme	polysaccharides	551:565	arg1	identification					526:539	the identification	522:539	the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP)	522:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	5	64	theme	smaller	1023:1029	arg1	volume					1031:1036	a smaller volume	1021:1036	a smaller volume of sterile seawater	1021:1056	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	1	65	from	microalgae	166:175	arg1	particles					100:108	Marine gel particles	89:108	Marine gel particles (MGP)	89:114	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	65	from	microalgae	166:175	arg1	exudates					139:146	amorphous hydrogel exudates	120:146	amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans	120:209	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	8	66	dep	localise	1303:1310	arg1	ConA					1295:1298	ConA	1295:1298	ConA	1295:1298	TOTO-3 was also used to stain eDNA, with ConA to localise glycoproteins and SYTO-9 for the live/dead staining of cells.
36975700	2	67	theme	compositional	475:487	arg1	studies					489:495	compositional studies	475:495	compositional studies	475:495	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	0	68	theme	Gel	74:76	arg1	Particles					78:86	Marine Gel Particles	67:86	Marine Gel Particles	67:86	Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles.
36975700	12	69	theme	dynamics	1817:1824	arg1	understanding					1784:1796	our understanding	1780:1796	our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean	1780:1929	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	1	70	from	bacteria	153:160	arg1	particles					100:108	Marine gel particles	89:108	Marine gel particles (MGP)	89:114	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	70	from	bacteria	153:160	arg1	exudates					139:146	amorphous hydrogel exudates	120:146	amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans	120:209	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	71	theme	hydrogel	130:137	arg1	particles					100:108	Marine gel particles	89:108	Marine gel particles (MGP)	89:114	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	71	theme	hydrogel	130:137	arg1	exudates					139:146	amorphous hydrogel exudates	120:146	amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans	120:209	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	12	72	theme	fate	1830:1833	arg1	understanding					1784:1796	our understanding	1780:1796	our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean	1780:1929	Our results clearly demonstrate the occurrence of eDNA in MGPs, and should aid furthering our understanding of the micro-scale dynamics and fate of MGPs that underly the large-scale processes of carbon cycling and sedimentation in the ocean.
36975700	9	73	theme	proteins	1441:1448	arg1	presence					1429:1436	the presence	1425:1436	the presence of proteins and polysaccharides	1425:1468	Confocal laser scanning microscopy (CLSM) revealed the presence of proteins and polysaccharides.
36975700	4	74	theme	isolating	744:752	arg1	MGPs					754:757	isolating MGPs	744:757	isolating MGPs	744:757	We developed a new way of isolating MGPs from seawater in liquid suspension and applied it to identify extracellular DNA (eDNA) in North Sea surface seawater.
36975700	5	75	theme	vacuum	943:948	arg1	filtration					950:959	gentle vacuum filtration	936:959	gentle vacuum filtration	936:959	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	0	76	from	Presence	34:41	arg1	Particles					78:86	Marine Gel Particles	67:86	Marine Gel Particles	67:86	Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles.
36975700	8	77	used	used	1270:1273	arg2	TOTO-3					1254:1259	TOTO-3	1254:1259	TOTO-3	1254:1259	TOTO-3 was also used to stain eDNA, with ConA to localise glycoproteins and SYTO-9 for the live/dead staining of cells.
36975700	1	78	theme	Marine	89:94	arg1	MGP					111:113	MGP	111:113	MGP	111:113	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	78	theme	Marine	89:94	arg1	particles					100:108	Marine gel particles	89:108	Marine gel particles (MGP)	89:114	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	1	78	theme	Marine	89:94	arg1	exudates					139:146	amorphous hydrogel exudates	120:146	amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans	120:209	Marine gel particles (MGP) are amorphous hydrogel exudates from bacteria and microalgae that are ubiquitous in the oceans, but their biochemical composition and function are poorly understood.
36975700	2	79	theme	polymeric	425:433	arg1	acids					468:472	nucleic acids	460:472	nucleic acids	460:472	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	79	theme	polymeric	425:433	arg1	EPS					447:449	EPS	447:449	EPS	447:449	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	79	theme	polymeric	425:433	arg1	substances					435:444	bacterial extracellular polymeric substances	401:444	bacterial extracellular polymeric substances (EPS) such as nucleic acids	401:472	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	11	80	theme	model	1581:1585	arg1	system					1604:1609	a model experimental MGP system	1579:1609	a model experimental MGP system	1579:1609	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	2	81	theme	bacterial	401:409	arg1	acids					468:472	nucleic acids	460:472	nucleic acids	460:472	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	81	theme	bacterial	401:409	arg1	EPS					447:449	EPS	447:449	EPS	447:449	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	81	theme	bacterial	401:409	arg1	substances					435:444	bacterial extracellular polymeric substances	401:444	bacterial extracellular polymeric substances (EPS) such as nucleic acids	401:472	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	5	82	theme	filtered	975:982	arg1	particles					984:992	then the filtered particles	966:992	then the filtered particles	966:992	Seawater was filtered onto polycarbonate (PC) filters with gentle vacuum filtration, and then the filtered particles were gently resuspended in a smaller volume of sterile seawater.
36975700	0	83	theme	Extracellular	46:58	arg1	DNA					60:62	Extracellular DNA	46:62	Extracellular DNA	46:62	Isolation and Staining Reveal the Presence of Extracellular DNA in Marine Gel Particles.
36975700	3	84	theme	Previous	663:670	arg1	studies					672:678	Previous studies	663:678	Previous studies	663:678	Previous studies targeted MGPs isolated by filtration.
36975700	2	85	theme	marine	328:333	arg1	microorganisms					335:348	marine microorganisms	328:348	marine microorganisms	328:348	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	86	from	identification	526:539	arg1	particles					606:614	transparent exopolymer particles	583:614	transparent exopolymer particles (TEP)	583:620	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	86	from	identification	526:539	arg1	CSP					657:659	CSP	657:659	CSP	657:659	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	86	from	identification	526:539	arg1	particles					646:654	Coomassie stainable particles	626:654	Coomassie stainable particles (CSP)	626:660	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	2	86	from	identification	526:539	arg1	TEP					617:619	TEP	617:619	TEP	617:619	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36975700	11	87	theme	MGP	1600:1602	arg1	system					1604:1609	a model experimental MGP system	1579:1609	a model experimental MGP system	1579:1609	To further elucidate the role of eDNA, we established a model experimental MGP system using bacterial EPS from Pseudoalteromonas atlantica that also contained eDNA.
36975700	2	88	dep	secretion	377:385	arg1	the					373:375	the	373:375	the	373:375	While dynamic ecological interactions between marine microorganisms and MGPs may result in the secretion and mixing of bacterial extracellular polymeric substances (EPS) such as nucleic acids, compositional studies currently are limited to the identification of acidic polysaccharides and proteins in transparent exopolymer particles (TEP) and Coomassie stainable particles (CSP).
36422855	9	0	theme	Bacteroidota	1315:1326	arg1	abundance					1302:1310	the relative abundance	1289:1310	the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice	1289:1367	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	4	1	from	effect	521:526	arg1	colitis					579:585	dextran sulfate sodium (DSS)-induced colitis	542:585	dextran sulfate sodium (DSS)-induced colitis	542:585	Therefore, the aim of our study was to investigate the anti-inflammatory effect of betaine in dextran sulfate sodium (DSS)-induced colitis.
36422855	7	2	theme	DSS-induced	972:982	arg1	phosphorylation					984:998	the DSS-induced phosphorylation	968:998	the DSS-induced phosphorylation of IκB and NF-κB p65 in mice	968:1027	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	10	3	theme	new	1552:1554	arg1	drugs					1556:1560	new drugs	1552:1560	new drugs for treating human diseases	1552:1588	In conclusion, betaine could attenuate colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota and is conducive to developing new drugs for treating human diseases.
36422855	4	4	theme	-induced	570:577	arg1	colitis					579:585	dextran sulfate sodium (DSS)-induced colitis	542:585	dextran sulfate sodium (DSS)-induced colitis	542:585	Therefore, the aim of our study was to investigate the anti-inflammatory effect of betaine in dextran sulfate sodium (DSS)-induced colitis.
36422855	0	5	theme	inflammatory	93:104	arg1	response					106:113	the inflammatory response	89:113	the inflammatory response	89:113	Betaine supplementation alleviates dextran sulfate sodium-induced colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.
36422855	1	6	theme	Inflammatory	179:190	arg1	IBD					207:209	IBD	207:209	IBD	207:209	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	1	6	theme	Inflammatory	179:190	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	1	6	theme	Inflammatory	179:190	arg1	disorder					251:258	a multifaceted and recurrent immune disorder	215:258	a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract	215:300	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	5	7	theme	body	642:645	arg1	weight					647:652	the body weight	638:652	the body weight	638:652	The results showed that betaine greatly increased the body weight and decreased the disease activity index score of DSS-treated mice.
36422855	4	8	theme	anti-inflammatory	503:519	arg1	effect					521:526	the anti-inflammatory effect	499:526	the anti-inflammatory effect of betaine in dextran sulfate sodium (DSS)-induced colitis	499:585	Therefore, the aim of our study was to investigate the anti-inflammatory effect of betaine in dextran sulfate sodium (DSS)-induced colitis.
36422855	10	9	theme	inflammatory	1436:1447	arg1	response					1449:1456	the inflammatory response	1432:1456	the inflammatory response	1432:1456	In conclusion, betaine could attenuate colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota and is conducive to developing new drugs for treating human diseases.
36422855	6	10	from	proteins	873:880	arg1	mice					909:912	the mice	905:912	the mice	905:912	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	4	11	theme	study	474:478	arg1	aim					463:465	the aim	459:465	the aim of our study	459:478	Therefore, the aim of our study was to investigate the anti-inflammatory effect of betaine in dextran sulfate sodium (DSS)-induced colitis.
36422855	7	12	theme	betaine	929:935	arg1	exposure					937:944	betaine exposure	929:944	betaine exposure	929:944	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	0	13	theme	intestinal	130:139	arg1	barrier					141:147	the intestinal barrier	126:147	the intestinal barrier	126:147	Betaine supplementation alleviates dextran sulfate sodium-induced colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.
36422855	9	14	theme	DSS-induced	1352:1362	arg1	mice					1364:1367	DSS-induced mice	1352:1367	DSS-induced mice	1352:1367	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	8	15	theme	betaine	1041:1047	arg1	pretreatment					1049:1060	betaine pretreatment	1041:1060	betaine pretreatment	1041:1060	Similarly, betaine pretreatment improved the inflammatory response and intestinal barrier of Caco-2 cells.
36422855	8	16	theme	intestinal	1101:1110	arg1	barrier					1112:1118	intestinal barrier	1101:1118	intestinal barrier	1101:1118	Similarly, betaine pretreatment improved the inflammatory response and intestinal barrier of Caco-2 cells.
36422855	9	17	dep	altered	1145:1151	arg1	decreasing					1194:1203	decreasing	1194:1203	markedly decreasing the relative abundance of Firmicutes and Proteobacteria	1185:1259	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	9	17	dep	altered	1145:1151	arg1	increasing					1278:1287	increasing	1278:1287	considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice	1265:1367	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	1	18	theme	bowel	192:196	arg1	IBD					207:209	IBD	207:209	IBD	207:209	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	1	18	theme	bowel	192:196	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	1	18	theme	bowel	192:196	arg1	disorder					251:258	a multifaceted and recurrent immune disorder	215:258	a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract	215:300	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	6	19	theme	cytokines	808:816	arg1	levels					781:786	the protein levels	769:786	the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα)	769:840	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	0	20	theme	Betaine	0:6	arg1	supplementation					8:22	Betaine supplementation	0:22	Betaine supplementation	0:22	Betaine supplementation alleviates dextran sulfate sodium-induced colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.
36422855	6	21	dep	cytokines	808:816	arg1	TNFα					836:839	TNFα	836:839	TNFα	836:839	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	21	dep	cytokines	808:816	arg1	IL-1β					819:823	IL-1β	819:823	IL-1β	819:823	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	21	dep	cytokines	808:816	arg1	cytokines					808:816	pro-inflammatory cytokines	791:816	pro-inflammatory cytokines (IL-1β, IL-6, and TNFα)	791:840	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	21	dep	cytokines	808:816	arg1	IL-6					826:829	IL-6	826:829	IL-6	826:829	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	10	22	theme	intestinal	1473:1482	arg1	barrier					1484:1490	the intestinal barrier	1469:1490	the intestinal barrier	1469:1490	In conclusion, betaine could attenuate colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota and is conducive to developing new drugs for treating human diseases.
36422855	6	23	theme	pro-inflammatory	791:806	arg1	TNFα					836:839	TNFα	836:839	TNFα	836:839	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	23	theme	pro-inflammatory	791:806	arg1	IL-1β					819:823	IL-1β	819:823	IL-1β	819:823	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	23	theme	pro-inflammatory	791:806	arg1	cytokines					808:816	pro-inflammatory cytokines	791:816	pro-inflammatory cytokines (IL-1β, IL-6, and TNFα)	791:840	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	23	theme	pro-inflammatory	791:806	arg1	IL-6					826:829	IL-6	826:829	IL-6	826:829	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	5	24	theme	disease	672:678	arg1	score					695:699	the disease activity index score	668:699	the disease activity index score of DSS-treated mice	668:719	The results showed that betaine greatly increased the body weight and decreased the disease activity index score of DSS-treated mice.
36422855	7	25	from	phosphorylation	984:998	arg1	mice					1024:1027	mice	1024:1027	mice	1024:1027	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	3	26	theme	betaine	404:410	arg1	role					396:399	the role	392:399	the role of betaine in protecting IBD	392:428	However, the role of betaine in protecting IBD is still unclear.
36422855	3	26	theme	betaine	404:410	arg1	unclear					439:445	unclear	439:445	unclear	439:445	However, the role of betaine in protecting IBD is still unclear.
36422855	1	27	theme	gastrointestinal	279:294	arg1	tract					296:300	the gastrointestinal tract	275:300	the gastrointestinal tract	275:300	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	10	28	theme	gut	1506:1508	arg1	microbiota					1510:1519	gut microbiota	1506:1519	gut microbiota	1506:1519	In conclusion, betaine could attenuate colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota and is conducive to developing new drugs for treating human diseases.
36422855	2	29	theme	natural	316:322	arg1	Betaine					303:309	Betaine	303:309	Betaine	303:309	Betaine is a natural compound that exerts beneficial anti-inflammatory effects.
36422855	2	29	theme	natural	316:322	arg1	compound					324:331	a natural compound	314:331	a natural compound that exerts beneficial anti-inflammatory effects	314:380	Betaine is a natural compound that exerts beneficial anti-inflammatory effects.
36422855	9	30	theme	Proteobacteria	1246:1259	arg1	abundance					1218:1226	the relative abundance	1205:1226	the relative abundance of Firmicutes and Proteobacteria	1205:1259	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	0	31	theme	dextran	35:41	arg1	sulfate					43:49	dextran sulfate	35:49	dextran sulfate sodium-induced colitis	35:72	Betaine supplementation alleviates dextran sulfate sodium-induced colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.
36422855	8	32	theme	inflammatory	1075:1086	arg1	response					1088:1095	inflammatory response	1075:1095	inflammatory response	1075:1095	Similarly, betaine pretreatment improved the inflammatory response and intestinal barrier of Caco-2 cells.
36422855	9	33	theme	Firmicutes	1231:1240	arg1	abundance					1218:1226	the relative abundance	1205:1226	the relative abundance of Firmicutes and Proteobacteria	1205:1259	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	8	34	dep	response	1088:1095	arg1	the					1071:1073	the	1071:1073	the	1071:1073	Similarly, betaine pretreatment improved the inflammatory response and intestinal barrier of Caco-2 cells.
36422855	7	35	from	IκB	1003:1005	arg1	mice					1024:1027	mice	1024:1027	mice	1024:1027	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	6	36	theme	junction	864:871	arg1	occludin					883:890	occludin	883:890	occludin	883:890	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	36	theme	junction	864:871	arg1	proteins					873:880	tight junction proteins	858:880	tight junction proteins (occludin and ZO-1) in the mice	858:912	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	36	theme	junction	864:871	arg1	ZO-1					896:899	ZO-1	896:899	ZO-1	896:899	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	0	37	theme	sodium-induced	51:64	arg1	colitis					66:72	dextran sulfate sodium-induced colitis	35:72	dextran sulfate sodium-induced colitis	35:72	Betaine supplementation alleviates dextran sulfate sodium-induced colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.
36422855	5	38	theme	DSS-treated	704:714	arg1	mice					716:719	DSS-treated mice	704:719	DSS-treated mice	704:719	The results showed that betaine greatly increased the body weight and decreased the disease activity index score of DSS-treated mice.
36422855	6	39	theme	tight	858:862	arg1	occludin					883:890	occludin	883:890	occludin	883:890	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	39	theme	tight	858:862	arg1	proteins					873:880	tight junction proteins	858:880	tight junction proteins (occludin and ZO-1) in the mice	858:912	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	39	theme	tight	858:862	arg1	ZO-1					896:899	ZO-1	896:899	ZO-1	896:899	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	40	dep	proteins	873:880	arg1	occludin					883:890	occludin	883:890	occludin	883:890	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	40	dep	proteins	873:880	arg1	proteins					873:880	tight junction proteins	858:880	tight junction proteins (occludin and ZO-1) in the mice	858:912	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	6	40	dep	proteins	873:880	arg1	ZO-1					896:899	ZO-1	896:899	ZO-1	896:899	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	0	41	theme	sulfate	43:49	arg1	colitis					66:72	dextran sulfate sodium-induced colitis	35:72	dextran sulfate sodium-induced colitis	35:72	Betaine supplementation alleviates dextran sulfate sodium-induced colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.
36422855	4	42	theme	betaine	531:537	arg1	effect					521:526	the anti-inflammatory effect	499:526	the anti-inflammatory effect of betaine in dextran sulfate sodium (DSS)-induced colitis	499:585	Therefore, the aim of our study was to investigate the anti-inflammatory effect of betaine in dextran sulfate sodium (DSS)-induced colitis.
36422855	5	43	theme	mice	716:719	arg1	score					695:699	the disease activity index score	668:699	the disease activity index score of DSS-treated mice	668:719	The results showed that betaine greatly increased the body weight and decreased the disease activity index score of DSS-treated mice.
36422855	6	44	theme	protein	773:779	arg1	levels					781:786	the protein levels	769:786	the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα)	769:840	Furthermore, betaine effectively downregulated the protein levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNFα) and upregulated tight junction proteins (occludin and ZO-1) in the mice.
36422855	1	45	theme	multifaceted	217:228	arg1	disorder					251:258	a multifaceted and recurrent immune disorder	215:258	a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract	215:300	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	1	45	theme	multifaceted	217:228	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	10	46	theme	human	1575:1579	arg1	diseases					1581:1588	human diseases	1575:1588	human diseases	1575:1588	In conclusion, betaine could attenuate colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota and is conducive to developing new drugs for treating human diseases.
36422855	9	47	from	abundance	1302:1310	arg1	mice					1364:1367	DSS-induced mice	1352:1367	DSS-induced mice	1352:1367	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	7	48	theme	p65	1017:1019	arg1	phosphorylation					984:998	the DSS-induced phosphorylation	968:998	the DSS-induced phosphorylation of IκB and NF-κB p65 in mice	968:1027	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	2	49	theme	anti-inflammatory	356:372	arg1	effects					374:380	beneficial anti-inflammatory effects	345:380	beneficial anti-inflammatory effects	345:380	Betaine is a natural compound that exerts beneficial anti-inflammatory effects.
36422855	9	50	theme	Campylobacterota	1332:1347	arg1	abundance					1302:1310	the relative abundance	1289:1310	the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice	1289:1367	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	0	51	theme	gut	163:165	arg1	microbiota					167:176	gut microbiota	163:176	gut microbiota	163:176	Betaine supplementation alleviates dextran sulfate sodium-induced colitis via regulating the inflammatory response, enhancing the intestinal barrier, and altering gut microbiota.
36422855	7	52	theme	IκB	1003:1005	arg1	phosphorylation					984:998	the DSS-induced phosphorylation	968:998	the DSS-induced phosphorylation of IκB and NF-κB p65 in mice	968:1027	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	2	53	theme	beneficial	345:354	arg1	effects					374:380	beneficial anti-inflammatory effects	345:380	beneficial anti-inflammatory effects	345:380	Betaine is a natural compound that exerts beneficial anti-inflammatory effects.
36422855	5	54	theme	activity	680:687	arg1	score					695:699	the disease activity index score	668:699	the disease activity index score of DSS-treated mice	668:719	The results showed that betaine greatly increased the body weight and decreased the disease activity index score of DSS-treated mice.
36422855	9	55	theme	relative	1209:1216	arg1	abundance					1218:1226	the relative abundance	1205:1226	the relative abundance of Firmicutes and Proteobacteria	1205:1259	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	5	56	theme	index	689:693	arg1	score					695:699	the disease activity index score	668:699	the disease activity index score of DSS-treated mice	668:719	The results showed that betaine greatly increased the body weight and decreased the disease activity index score of DSS-treated mice.
36422855	9	57	theme	relative	1293:1300	arg1	abundance					1302:1310	the relative abundance	1289:1310	the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice	1289:1367	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	7	58	theme	NF-κB	1011:1015	arg1	p65					1017:1019	NF-κB p65	1011:1019	NF-κB p65	1011:1019	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	8	59	theme	Caco-2	1123:1128	arg1	cells					1130:1134	Caco-2 cells	1123:1134	Caco-2 cells	1123:1134	Similarly, betaine pretreatment improved the inflammatory response and intestinal barrier of Caco-2 cells.
36422855	1	60	theme	recurrent	234:242	arg1	disorder					251:258	a multifaceted and recurrent immune disorder	215:258	a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract	215:300	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	1	60	theme	recurrent	234:242	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	7	61	from	p65	1017:1019	arg1	mice					1024:1027	mice	1024:1027	mice	1024:1027	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	9	62	theme	gut	1157:1159	arg1	composition					1172:1182	the gut microbiota composition	1153:1182	the gut microbiota composition	1153:1182	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
36422855	1	63	theme	immune	244:249	arg1	disorder					251:258	a multifaceted and recurrent immune disorder	215:258	a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract	215:300	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	1	63	theme	immune	244:249	arg1	disease					198:204	Inflammatory bowel disease	179:204	Inflammatory bowel disease (IBD)	179:210	Inflammatory bowel disease (IBD) is a multifaceted and recurrent immune disorder that occurs in the gastrointestinal tract.
36422855	8	64	theme	cells	1130:1134	arg1	response					1088:1095	inflammatory response	1075:1095	inflammatory response	1075:1095	Similarly, betaine pretreatment improved the inflammatory response and intestinal barrier of Caco-2 cells.
36422855	8	64	theme	cells	1130:1134	arg1	barrier					1112:1118	intestinal barrier	1101:1118	intestinal barrier	1101:1118	Similarly, betaine pretreatment improved the inflammatory response and intestinal barrier of Caco-2 cells.
36422855	7	65	from	mice	1024:1027	arg1	phosphorylation					984:998	the DSS-induced phosphorylation	968:998	the DSS-induced phosphorylation of IκB and NF-κB p65 in mice	968:1027	Additionally, betaine exposure remarkably restricted the DSS-induced phosphorylation of IκB and NF-κB p65 in mice.
36422855	9	66	theme	microbiota	1161:1170	arg1	composition					1172:1182	the gut microbiota composition	1153:1182	the gut microbiota composition	1153:1182	Betaine altered the gut microbiota composition, markedly decreasing the relative abundance of Firmicutes and Proteobacteria and considerably increasing the relative abundance of Bacteroidota and Campylobacterota in DSS-induced mice.
37204832	11	0	theme	average	1533:1539	arg1	identity					1552:1559	average nucleotide identity	1533:1559	average nucleotide identity	1533:1559	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	7	1	theme	5-21aT	1059:1064	arg1	gene					1044:1047	the 16S rRNA gene	1031:1047	the 16S rRNA gene of strain 5-21aT	1031:1064	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	9	2	theme	cellular	1329:1336	arg1	iso-C15:0					1355:1363	iso-C15:0	1355:1363	iso-C15:0	1355:1363	The predominant cellular fatty acids were iso-C15:0, iso-C16:0 and iso-C17:1 ω9c.
37204832	9	2	theme	cellular	1329:1336	arg1	acids					1344:1348	The predominant cellular fatty acids	1313:1348	The predominant cellular fatty acids	1313:1348	The predominant cellular fatty acids were iso-C15:0, iso-C16:0 and iso-C17:1 ω9c.
37204832	8	3	theme	principal	1274:1282	arg1	Q-8					1308:1310	Q-8	1308:1310	Q-8	1308:1310	The principal respiratory quinone was Q-8.
37204832	8	3	theme	principal	1274:1282	arg1	quinone					1296:1302	The principal respiratory quinone	1270:1302	The principal respiratory quinone	1270:1302	The principal respiratory quinone was Q-8.
37204832	11	4	theme	hybridization	1581:1593	arg1	%					1700:1700	88.8 and 36.5%	1687:1700	%	1700:1700	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	11	4	theme	hybridization	1581:1593	arg1	values					1595:1600	The average nucleotide identity and digital DNA-DNA hybridization values	1529:1600	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T	1529:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	11	5	theme	digital	1565:1571	arg1	hybridization					1581:1593	digital DNA-DNA hybridization	1565:1593	digital DNA-DNA hybridization	1565:1593	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	7	6	theme	rRNA	1039:1042	arg1	gene					1044:1047	the 16S rRNA gene	1031:1047	the 16S rRNA gene of strain 5-21aT	1031:1064	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	4	7	contain	possesses	531:539	arg1	5-21aT					524:529	strain 5-21aT	517:529	strain 5-21aT	517:529	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	4	7	contain	possesses	531:539	arg2	gene					559:562	only the putative gene	541:562	only the putative gene for Cbl-dependent Met synthase (MetH)	541:600	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	12	8	theme	name	1865:1868	arg1	sp					1894:1895	the name Lyobacter auxotrophicus sp	1861:1895	the name Lyobacter auxotrophicus sp	1861:1895	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	12	9	theme	auxotrophicus	1880:1892	arg1	sp					1894:1895	the name Lyobacter auxotrophicus sp	1861:1895	the name Lyobacter auxotrophicus sp	1861:1895	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	7	10	theme	copies	1021:1026	arg1	sequences					1004:1012	The nucleotide sequences	989:1012	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT	989:1064	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	5	11	theme	synthesis	799:807	arg1	pathway					784:790	the upstream (corrin ring synthesis) pathway	747:790	the upstream (corrin ring synthesis) pathway of Cbl synthesis	747:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	11	theme	synthesis	799:807	arg1	synthesis					773:781	corrin ring synthesis	761:781	corrin ring synthesis	761:781	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	4	12	theme	Cbl	686:688	arg1	requirement					671:681	the requirement	667:681	the requirement of Cbl for Met-synthesis in strain 5-21aT	667:723	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	10	13	theme	67.87 mol	1517:1525	arg1	content					1505:1511	the G+C content	1497:1511	the G+C content	1497:1511	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	10	13	theme	67.87 mol	1517:1525	arg1	%					1526:1526	67.87 mol%	1517:1526	67.87 mol%	1517:1526	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	7	14	theme	nucleotide	993:1002	arg1	sequences					1004:1012	The nucleotide sequences	989:1012	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT	989:1064	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	11	15	theme	soli	1670:1673	arg1	DCY21T					1675:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	1	16	theme	methionine-auxotrophic	113:134	arg1	bacterium					149:157	a methionine-auxotrophic chitinolytic bacterium	111:157	a methionine-auxotrophic chitinolytic bacterium	111:157	nov., a methionine-auxotrophic chitinolytic bacterium isolated from chitin-treated soil.
37204832	1	16	theme	methionine-auxotrophic	113:134	arg1	nov.					105:108	nov.	105:108	nov.	105:108	nov., a methionine-auxotrophic chitinolytic bacterium isolated from chitin-treated soil.
37204832	11	17	theme	relative	1658:1665	arg1	DCY21T					1675:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	2	18	theme	chitin-treated	246:259	arg1	soil					268:271	chitin-treated upland soil	246:271	chitin-treated upland soil	246:271	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	12	19	from	species	1818:1824	arg1	Lysobacter					1839:1848	the genus Lysobacter	1829:1848	the genus Lysobacter	1829:1848	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	5	20	theme	genes	737:741	arg1	set					730:732	The set	726:732	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis	726:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	20	theme	genes	737:741	arg1	absent					812:817	absent	812:817	absent	812:817	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	20	theme	genes	737:741	arg1	genes					737:741	genes	737:741	genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis	737:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	21	theme	5-21aT	843:848	arg1	genome					826:831	the genome	822:831	the genome	822:831	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	6	22	theme	polyphasic	932:941	arg1	approach					943:950	a polyphasic approach	930:950	a polyphasic approach to determine its taxonomic position	930:986	This strain was characterized via a polyphasic approach to determine its taxonomic position.
37204832	0	23	theme	Lysobacter	76:85	arg1	sp					101:102	Lysobacter auxotrophicus sp	76:102	Lysobacter auxotrophicus sp	76:102	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	5	24	from	genome	826:831	arg1	absent					812:817	absent	812:817	absent	812:817	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	24	from	genome	826:831	arg1	genes					737:741	genes	737:741	genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis	737:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	24	from	genome	826:831	arg1	set					730:732	The set	726:732	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis	726:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	14	25	theme	115507T=LMG	1949:1959	arg1	5-21aT					1935:1940	5-21aT	1935:1940	5-21aT (=NBRC 115507T=LMG 32660T)	1935:1967	The type strain is 5-21aT (=NBRC 115507T=LMG 32660T).
37204832	14	25	theme	115507T=LMG	1949:1959	arg1	32660T					1961:1966	=NBRC 115507T=LMG 32660T	1943:1966	=NBRC 115507T=LMG 32660T	1943:1966	The type strain is 5-21aT (=NBRC 115507T=LMG 32660T).
37204832	0	26	theme	sp	101:102	arg1	-auxotrophy					61:71	-auxotrophy	61:71	-auxotrophy of Lysobacter auxotrophicus sp	61:102	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	0	27	theme	cobalamin	38:46	arg1	analyses					26:33	Physiological and genomic analyses	0:33	Physiological and genomic analyses of cobalamin (vitamin B12)	0:60	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	4	28	theme	complete	476:483	arg1	sequence					493:500	The newly determined complete genomic sequence	455:500	The newly determined complete genomic sequence	455:500	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	4	29	theme	strain	711:716	arg1	5-21aT					718:723	strain 5-21aT	711:723	strain 5-21aT	711:723	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	0	30	theme	Physiological	0:12	arg1	analyses					26:33	Physiological and genomic analyses	0:33	Physiological and genomic analyses of cobalamin (vitamin B12)	0:60	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	0	31	dep	-auxotrophy	61:71	arg1	analyses					26:33	Physiological and genomic analyses	0:33	Physiological and genomic analyses of cobalamin (vitamin B12)	0:60	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	4	32	theme	putative	550:557	arg1	gene					559:562	only the putative gene	541:562	only the putative gene for Cbl-dependent Met synthase (MetH)	541:600	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	0	33	theme	genomic	18:24	arg1	analyses					26:33	Physiological and genomic analyses	0:33	Physiological and genomic analyses of cobalamin (vitamin B12)	0:60	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	12	34	theme	phylogenetic	1766:1777	arg1	data					1779:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	10	35	theme	complete	1399:1406	arg1	sequence					1415:1422	The complete genome sequence	1395:1422	The complete genome sequence of strain 5-21aT	1395:1439	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	3	36	theme	strain	440:445	arg1	5-21aT					447:452	strain 5-21aT	440:452	strain 5-21aT	440:452	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	11	37	theme	strain	1610:1615	arg1	5-21aT					1617:1622	strain 5-21aT	1610:1622	strain 5-21aT	1610:1622	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	12	38	theme	chemotaxonomic	1735:1748	arg1	data					1779:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	10	39	theme	strain	1427:1432	arg1	5-21aT					1434:1439	strain 5-21aT	1427:1439	strain 5-21aT	1427:1439	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	11	40	theme	phylogenetic	1645:1656	arg1	DCY21T					1675:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	12	41	theme	phenotypic	1751:1760	arg1	data					1779:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	2	42	theme	novel	196:200	arg1	bacterium					202:210	A novel bacterium	194:210	A novel bacterium	194:210	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	5	43	theme	upstream	751:758	arg1	pathway					784:790	the upstream (corrin ring synthesis) pathway	747:790	the upstream (corrin ring synthesis) pathway of Cbl synthesis	747:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	43	theme	upstream	751:758	arg1	synthesis					773:781	corrin ring synthesis	761:781	corrin ring synthesis	761:781	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	7	44	theme	strain	1052:1057	arg1	5-21aT					1059:1064	strain 5-21aT	1052:1064	strain 5-21aT	1052:1064	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	9	45	theme	predominant	1317:1327	arg1	iso-C15:0					1355:1363	iso-C15:0	1355:1363	iso-C15:0	1355:1363	The predominant cellular fatty acids were iso-C15:0, iso-C16:0 and iso-C17:1 ω9c.
37204832	9	45	theme	predominant	1317:1327	arg1	acids					1344:1348	The predominant cellular fatty acids	1313:1348	The predominant cellular fatty acids	1313:1348	The predominant cellular fatty acids were iso-C15:0, iso-C16:0 and iso-C17:1 ω9c.
37204832	4	46	theme	Met	582:584	arg1	MetH					596:599	MetH	596:599	MetH	596:599	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	4	46	theme	Met	582:584	arg1	synthase					586:593	Cbl-dependent Met synthase	568:593	Cbl-dependent Met synthase (MetH)	568:600	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	11	47	theme	nucleotide	1541:1550	arg1	identity					1552:1559	average nucleotide identity	1533:1559	average nucleotide identity	1533:1559	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	7	48	theme	gene	1044:1047	arg1	copies					1021:1026	two copies	1017:1026	two copies of the 16S rRNA gene of strain 5-21aT	1017:1064	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	2	49	theme	Met	295:297	arg1	auxotrophy					300:309	methionine (Met) auxotrophy	283:309	methionine (Met) auxotrophy	283:309	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	9	50	theme	fatty	1338:1342	arg1	iso-C15:0					1355:1363	iso-C15:0	1355:1363	iso-C15:0	1355:1363	The predominant cellular fatty acids were iso-C15:0, iso-C16:0 and iso-C17:1 ω9c.
37204832	9	50	theme	fatty	1338:1342	arg1	acids					1344:1348	The predominant cellular fatty acids	1313:1348	The predominant cellular fatty acids	1313:1348	The predominant cellular fatty acids were iso-C15:0, iso-C16:0 and iso-C17:1 ω9c.
37204832	7	51	theme	16S	1035:1037	arg1	rRNA					1039:1042	the 16S rRNA	1031:1042	the 16S rRNA gene of strain 5-21aT	1031:1064	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	2	52	theme	methionine	283:292	arg1	auxotrophy					300:309	methionine (Met) auxotrophy	283:309	methionine (Met) auxotrophy	283:309	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	1	53	theme	chitin-treated	173:186	arg1	soil					188:191	chitin-treated soil	173:191	chitin-treated soil	173:191	nov., a methionine-auxotrophic chitinolytic bacterium isolated from chitin-treated soil.
37204832	11	54	theme	DNA-DNA	1573:1579	arg1	hybridization					1581:1593	digital DNA-DNA hybridization	1565:1593	digital DNA-DNA hybridization	1565:1593	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	2	55	theme	chitinolytic	315:326	arg1	activity					328:335	chitinolytic activity	315:335	chitinolytic activity	315:335	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	10	56	theme	G+C	1501:1503	arg1	content					1505:1511	the G+C content	1497:1511	the G+C content	1497:1511	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	10	56	theme	G+C	1501:1503	arg1	%					1526:1526	67.87 mol%	1517:1526	67.87 mol%	1517:1526	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	12	57	theme	Lyobacter	1870:1878	arg1	sp					1894:1895	the name Lyobacter auxotrophicus sp	1861:1895	the name Lyobacter auxotrophicus sp	1861:1895	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	5	58	theme	Cbl	795:797	arg1	synthesis					799:807	Cbl synthesis	795:807	Cbl synthesis	795:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	4	59	theme	Cbl-independent	625:639	arg1	MetE					646:649	MetE	646:649	MetE	646:649	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	4	59	theme	Cbl-independent	625:639	arg1	one					641:643	the Cbl-independent one	621:643	the Cbl-independent one (MetE)	621:650	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	6	60	theme	taxonomic	969:977	arg1	position					979:986	its taxonomic position	965:986	its taxonomic position	965:986	This strain was characterized via a polyphasic approach to determine its taxonomic position.
37204832	2	61	theme	upland	261:266	arg1	soil					268:271	chitin-treated upland soil	246:271	chitin-treated upland soil	246:271	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	1	62	theme	chitinolytic	136:147	arg1	bacterium					149:157	a methionine-auxotrophic chitinolytic bacterium	111:157	a methionine-auxotrophic chitinolytic bacterium	111:157	nov., a methionine-auxotrophic chitinolytic bacterium isolated from chitin-treated soil.
37204832	1	62	theme	chitinolytic	136:147	arg1	nov.					105:108	nov.	105:108	nov.	105:108	nov., a methionine-auxotrophic chitinolytic bacterium isolated from chitin-treated soil.
37204832	3	63	theme	physiological	340:352	arg1	experiment					354:363	A physiological experiment	338:363	A physiological experiment	338:363	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	11	64	theme	L.	1667:1668	arg1	DCY21T					1675:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	its most closely phylogenetic relative L. soli DCY21T	1628:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	5	65	from	absent	812:817	arg1	genome					826:831	the genome	822:831	the genome	822:831	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	66	theme	strain	836:841	arg1	5-21aT					843:848	strain 5-21aT	836:848	strain 5-21aT	836:848	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	2	67	attach	isolated	232:239	arg2	bacterium					202:210	A novel bacterium	194:210	A novel bacterium	194:210	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	2	67	attach	isolated	232:239	arg1	soil					268:271	chitin-treated upland soil	246:271	chitin-treated upland soil	246:271	A novel bacterium, designated 5-21aT, isolated from chitin-treated upland soil, exhibits methionine (Met) auxotrophy and chitinolytic activity.
37204832	4	68	theme	strain	517:522	arg1	5-21aT					524:529	strain 5-21aT	517:529	strain 5-21aT	517:529	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	11	69	theme	36.5	1696:1699	arg1	%					1700:1700	88.8 and 36.5%	1687:1700	%	1700:1700	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	11	69	theme	36.5	1696:1699	arg1	values					1595:1600	The average nucleotide identity and digital DNA-DNA hybridization values	1529:1600	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T	1529:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	0	70	theme	auxotrophicus	87:99	arg1	sp					101:102	Lysobacter auxotrophicus sp	76:102	Lysobacter auxotrophicus sp	76:102	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	12	71	theme	genus	1833:1837	arg1	Lysobacter					1839:1848	the genus Lysobacter	1829:1848	the genus Lysobacter	1829:1848	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	14	72	theme	type	1920:1923	arg1	5-21aT					1935:1940	5-21aT	1935:1940	5-21aT (=NBRC 115507T=LMG 32660T)	1935:1967	The type strain is 5-21aT (=NBRC 115507T=LMG 32660T).
37204832	14	72	theme	type	1920:1923	arg1	strain					1925:1930	The type strain	1916:1930	The type strain	1916:1930	The type strain is 5-21aT (=NBRC 115507T=LMG 32660T).
37204832	3	73	theme	cobalamin	378:386	arg1	property					428:435	the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property	374:435	the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT	374:452	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	4	74	theme	determined	465:474	arg1	sequence					493:500	The newly determined complete genomic sequence	455:500	The newly determined complete genomic sequence	455:500	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	7	75	dep	DCY21T	1120:1125	arg1	%					1190:1190	98.7 and 98.8 %	1176:1190	98.7 and 98.8 %	1176:1190	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	7	75	dep	DCY21T	1120:1125	arg1	99.8					1127:1130	99.8	1127:1130	99.8	1127:1130	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	7	75	dep	DCY21T	1120:1125	arg1	%					1141:1141	99.9 %	1136:1141	99.9 %	1136:1141	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	11	76	theme	88.8	1687:1690	arg1	%					1700:1700	88.8 and 36.5%	1687:1700	%	1700:1700	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	11	76	theme	88.8	1687:1690	arg1	values					1595:1600	The average nucleotide identity and digital DNA-DNA hybridization values	1529:1600	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T	1529:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	4	77	theme	genomic	485:491	arg1	sequence					493:500	The newly determined complete genomic sequence	455:500	The newly determined complete genomic sequence	455:500	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	5	78	theme	corrin	761:766	arg1	pathway					784:790	the upstream (corrin ring synthesis) pathway	747:790	the upstream (corrin ring synthesis) pathway of Cbl synthesis	747:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	78	theme	corrin	761:766	arg1	synthesis					773:781	corrin ring synthesis	761:781	corrin ring synthesis	761:781	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	12	79	theme	novel	1812:1816	arg1	species					1818:1824	a novel species	1810:1824	a novel species	1810:1824	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	7	80	theme	Cbl-auxotrophic	1214:1228	arg1	properties					1230:1239	Cbl-auxotrophic properties	1214:1239	Cbl-auxotrophic properties	1214:1239	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	3	81	theme	vitamin	398:404	arg1	synonym					389:395	synonym	389:395	synonym	389:395	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	3	81	theme	vitamin	398:404	arg1	B12					406:408	vitamin B12	398:408	vitamin B12	398:408	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	3	82	theme	-auxotrophic	415:426	arg1	property					428:435	the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property	374:435	the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT	374:452	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	5	83	theme	ring	768:771	arg1	pathway					784:790	the upstream (corrin ring synthesis) pathway	747:790	the upstream (corrin ring synthesis) pathway of Cbl synthesis	747:807	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	83	theme	ring	768:771	arg1	synthesis					773:781	corrin ring synthesis	761:781	corrin ring synthesis	761:781	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	5	84	theme	5-21aT	888:893	arg1	Cbl-auxotrophy					870:883	the Cbl-auxotrophy	866:883	the Cbl-auxotrophy of 5-21aT	866:893	The set of genes for the upstream (corrin ring synthesis) pathway of Cbl synthesis is absent in the genome of strain 5-21aT, which explains the Cbl-auxotrophy of 5-21aT.
37204832	4	85	theme	Cbl-dependent	568:580	arg1	MetH					596:599	MetH	596:599	MetH	596:599	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	4	85	theme	Cbl-dependent	568:580	arg1	synthase					586:593	Cbl-dependent Met synthase	568:593	Cbl-dependent Met synthase (MetH)	568:600	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	10	86	theme	genome	1408:1413	arg1	sequence					1415:1422	The complete genome sequence	1395:1422	The complete genome sequence of strain 5-21aT	1395:1439	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	8	87	theme	respiratory	1284:1294	arg1	Q-8					1308:1310	Q-8	1308:1310	Q-8	1308:1310	The principal respiratory quinone was Q-8.
37204832	8	87	theme	respiratory	1284:1294	arg1	quinone					1296:1302	The principal respiratory quinone	1270:1302	The principal respiratory quinone	1270:1302	The principal respiratory quinone was Q-8.
37204832	7	88	theme	highest	1080:1086	arg1	similarities					1088:1099	the highest similarities	1076:1099	the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study	1076:1267	The nucleotide sequences of two copies of the 16S rRNA gene of strain 5-21aT indicated the highest similarities to Lysobacter soli DCY21T(99.8 and 99.9 %) and Lysobacter panacisoli CJ29T(98.7 and 98.8 %, respectively), whose Cbl-auxotrophic properties were revealed in this study.
37204832	12	89	theme	strain	1785:1790	arg1	5-21aT					1792:1797	strain 5-21aT	1785:1797	strain 5-21aT	1785:1797	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	14	90	theme	=NBRC	1943:1947	arg1	5-21aT					1935:1940	5-21aT	1935:1940	5-21aT (=NBRC 115507T=LMG 32660T)	1935:1967	The type strain is 5-21aT (=NBRC 115507T=LMG 32660T).
37204832	14	90	theme	=NBRC	1943:1947	arg1	32660T					1961:1966	=NBRC 115507T=LMG 32660T	1943:1966	=NBRC 115507T=LMG 32660T	1943:1966	The type strain is 5-21aT (=NBRC 115507T=LMG 32660T).
37204832	3	91	theme	5-21aT	447:452	arg1	property					428:435	the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property	374:435	the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT	374:452	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	0	92	theme	vitamin	49:55	arg1	cobalamin					38:46	cobalamin	38:46	cobalamin (vitamin B12)	38:60	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	0	92	theme	vitamin	49:55	arg1	B12					57:59	vitamin B12	49:59	vitamin B12	49:59	Physiological and genomic analyses of cobalamin (vitamin B12)-auxotrophy of Lysobacter auxotrophicus sp.
37204832	10	93	theme	5-21aT	1434:1439	arg1	sequence					1415:1422	The complete genome sequence	1395:1422	The complete genome sequence of strain 5-21aT	1395:1439	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	9	94	dep	iso-C15:0	1355:1363	arg1	ω9c					1390:1392	ω9c	1390:1392	ω9c	1390:1392	The predominant cellular fatty acids were iso-C15:0, iso-C16:0 and iso-C17:1 ω9c.
37204832	4	95	from	Met-synthesis	694:706	arg1	5-21aT					718:723	strain 5-21aT	711:723	strain 5-21aT	711:723	The newly determined complete genomic sequence indicated that strain 5-21aT possesses only the putative gene for Cbl-dependent Met synthase (MetH) and lacks that for the Cbl-independent one (MetE), which implies the requirement of Cbl for Met-synthesis in strain 5-21aT.
37204832	12	96	theme	genomic	1726:1732	arg1	data					1779:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	genomic, chemotaxonomic, phenotypic and phylogenetic data	1726:1782	Based on genomic, chemotaxonomic, phenotypic and phylogenetic data, strain 5-21aT represents a novel species in the genus Lysobacter, for which the name Lyobacter auxotrophicus sp.
37204832	3	97	dep	cobalamin	378:386	arg1	synonym					389:395	synonym	389:395	synonym	389:395	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	3	97	dep	cobalamin	378:386	arg1	B12					406:408	vitamin B12	398:408	vitamin B12	398:408	A physiological experiment revealed the cobalamin (synonym, vitamin B12)(Cbl)-auxotrophic property of strain 5-21aT.
37204832	10	98	theme	genome	1459:1464	arg1	size					1466:1469	the genome size	1455:1469	the genome size	1455:1469	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	10	98	theme	genome	1459:1464	arg1	long					1488:1491	long	1488:1491	long	1488:1491	The complete genome sequence of strain 5-21aT revealed that the genome size was 4 155 451 bp long and the G+C content was 67.87 mol%.
37204832	11	99	theme	identity	1552:1559	arg1	%					1700:1700	88.8 and 36.5%	1687:1700	%	1700:1700	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
37204832	11	99	theme	identity	1552:1559	arg1	values					1595:1600	The average nucleotide identity and digital DNA-DNA hybridization values	1529:1600	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T	1529:1680	The average nucleotide identity and digital DNA-DNA hybridization values between strain 5-21aT and its most closely phylogenetic relative L. soli DCY21T were 88.8 and 36.5%, respectively.
36920836	10	0	dep	FJAT-49705T	981:991	arg1	FJAT-18043T					1038:1048	FJAT-18043T	1038:1048	FJAT-18043T	1038:1048	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	5	1	theme	16S	508:510	arg1	sequences					522:530	16S rRNA gene sequences	508:530	16S rRNA gene sequences	508:530	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36920836	8	2	theme	major	832:836	arg1	anteiso-C15 					855:866	anteiso-C15 	855:866	anteiso-C15 	855:866	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
36920836	8	2	theme	major	832:836	arg1	acids					844:848	The major fatty acids	828:848	The major fatty acids	828:848	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
36920836	9	3	theme	G+C	904:906	arg1	content					908:914	The genomic DNA G+C content	888:914	The genomic DNA G+C content	888:914	The genomic DNA G+C content was 36.9 %.
36920836	9	3	theme	G+C	904:906	arg1	%					925:925	36.9 %	920:925	36.9 %	920:925	The genomic DNA G+C content was 36.9 %.
36920836	4	4	theme	dafuensis	404:412	arg1	FJAT-25496T					415:425	'Bacillus dafuensis' FJAT-25496T	394:425	'Bacillus dafuensis' FJAT-25496T (99.7 %)	394:434	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	4	4	theme	dafuensis	404:412	arg1	%					433:433	99.7 %	428:433	99.7 %	428:433	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	4	5	theme	Strain	326:331	arg1	FJAT-49705T					333:343	Strain FJAT-49705T	326:343	Strain FJAT-49705T	326:343	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	6	6	theme	isoprenoid	690:699	arg1	MK-7					672:675	MK-7	672:675	MK-7	672:675	MK-7 was the only isoprenoid quinone present.
36920836	6	6	theme	isoprenoid	690:699	arg1	present					709:715	the only isoprenoid quinone present	681:715	the only isoprenoid quinone present	681:715	MK-7 was the only isoprenoid quinone present.
36920836	4	7	theme	solani	453:458	arg1	FJAT-18043T					460:470	Cytobacillus solani FJAT-18043T	440:470	Cytobacillus solani FJAT-18043T (98.0 %)	440:479	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	4	7	theme	solani	453:458	arg1	%					478:478	98.0 %	473:478	98.0 %	473:478	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	5	8	theme	bacterial	569:577	arg1	genes					591:595	71 bacterial single-copy genes	566:595	71 bacterial single-copy genes	566:595	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36920836	5	9	theme	gene	517:520	arg1	sequences					522:530	16S rRNA gene sequences	508:530	16S rRNA gene sequences	508:530	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36920836	1	10	theme	citrus	45:50	arg1	soil					64:67	citrus rhizosphere soil	45:67	citrus rhizosphere soil	45:67	nov., isolated from citrus rhizosphere soil.
36920836	7	11	theme	main	722:725	arg1	diphosphatidylglycerol					745:766	diphosphatidylglycerol	745:766	diphosphatidylglycerol	745:766	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
36920836	7	11	theme	main	722:725	arg1	lipids					733:738	The main polar lipids	718:738	The main polar lipids	718:738	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
36920836	3	12	theme	%	297:297	arg1	optimum					311:317	optimum	311:317	optimum	311:317	Strain FJAT-49705T grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %).
36920836	3	12	theme	%	297:297	arg1	NaCl					305:308	0-5 % (w/v) NaCl	293:308	0-5 % (w/v) NaCl (optimum, 2 %)	293:323	Strain FJAT-49705T grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %).
36920836	4	13	theme	gene	366:369	arg1	similarity					380:389	high 16S rRNA gene sequence similarity	352:389	high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %)	352:479	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	1	14	theme	rhizosphere	52:62	arg1	soil					64:67	citrus rhizosphere soil	45:67	citrus rhizosphere soil	45:67	nov., isolated from citrus rhizosphere soil.
36920836	10	15	theme	ANI	1108:1110	arg1	values					966:971	The average nucleotide identity (ANI) values	928:971	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T	928:1048	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	10	15	theme	ANI	1108:1110	arg1	criterion					1112:1120	the ANI criterion	1104:1120	the ANI criterion for interspecies identity	1104:1146	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	11	16	theme	genus	1230:1234	arg1	Cytobacillus					1236:1247	the genus Cytobacillus	1226:1247	the genus Cytobacillus	1226:1247	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	4	17	theme	rRNA	361:364	arg1	similarity					380:389	high 16S rRNA gene sequence similarity	352:389	high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %)	352:479	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	10	18	theme	nucleotide	940:949	arg1	ANI					961:963	ANI	961:963	ANI	961:963	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	10	18	theme	nucleotide	940:949	arg1	identity					951:958	The average nucleotide identity	928:958	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T	928:1048	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	3	19	dep	optimum	311:317	arg1	%					322:322	2 %	320:322	2 %	320:322	Strain FJAT-49705T grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %).
36920836	7	20	theme	unidentified	801:812	arg1	phospholipid					814:825	an unidentified phospholipid	798:825	an unidentified phospholipid	798:825	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
36920836	0	21	theme	Cytobacillus	0:11	arg1	sp					21:22	Cytobacillus citreus sp	0:22	Cytobacillus citreus sp.	0:23	Cytobacillus citreus sp.
36920836	11	22	theme	Cytobacillus	1236:1247	arg1	species					1215:1221	a novel species	1207:1221	a novel species	1207:1221	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	0	23	theme	citreus	13:19	arg1	sp					21:22	Cytobacillus citreus sp	0:22	Cytobacillus citreus sp.	0:23	Cytobacillus citreus sp.
36920836	4	24	theme	sequence	371:378	arg1	similarity					380:389	high 16S rRNA gene sequence similarity	352:389	high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %)	352:479	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	10	25	dep	B.	998:999	arg1	dafuensis					1001:1009	dafuensis	1001:1009	dafuensis	1001:1009	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	10	26	theme	identity	951:958	arg1	values					966:971	The average nucleotide identity (ANI) values	928:971	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T	928:1048	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	10	26	theme	identity	951:958	arg1	criterion					1112:1120	the ANI criterion	1104:1120	the ANI criterion for interspecies identity	1104:1146	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	13	27	theme	type	1316:1319	arg1	FJAT-49705T					1331:1341	FJAT-49705T	1331:1341	FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T)	1331:1374	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	13	27	theme	type	1316:1319	arg1	strain					1321:1326	The type strain	1312:1326	The type strain	1312:1326	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	2	28	theme	motile	108:113	arg1	strain					115:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	11	29	dep	Cytobacillus	1269:1280	arg1	citreus					1282:1288	citreus	1282:1288	citreus	1282:1288	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	11	30	theme	Cytobacillus	1269:1280	arg1	sp					1290:1291	the name Cytobacillus citreus sp	1260:1291	the name Cytobacillus citreus sp	1260:1291	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	5	31	theme	rRNA	512:515	arg1	sequences					522:530	16S rRNA gene sequences	508:530	16S rRNA gene sequences	508:530	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36920836	5	32	theme	Cytobacillus	658:669	arg1	members					637:643	the members	633:643	the members of the genus Cytobacillus	633:669	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36920836	5	33	theme	genus	652:656	arg1	Cytobacillus					658:669	the genus Cytobacillus	648:669	the genus Cytobacillus	648:669	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36920836	3	34	dep	optimum	271:277	arg1	pH					280:281	pH 7.0	280:285	pH 7.0	280:285	Strain FJAT-49705T grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %).
36920836	8	35	dep	anteiso-C15 	855:866	arg1	 0					884:885	 0	884:885	anteiso-C15 : 0 and iso-C15 : 0	855:885	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
36920836	8	35	dep	anteiso-C15 	855:866	arg1	iso-C15 					875:882	iso-C15 	875:882	iso-C15 	875:882	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
36920836	8	35	dep	anteiso-C15 	855:866	arg1	 0					868:869	 0	868:869	 0	868:869	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
36920836	3	36	theme	w/v	300:302	arg1	optimum					311:317	optimum	311:317	optimum	311:317	Strain FJAT-49705T grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %).
36920836	3	36	theme	w/v	300:302	arg1	NaCl					305:308	0-5 % (w/v) NaCl	293:308	0-5 % (w/v) NaCl (optimum, 2 %)	293:323	Strain FJAT-49705T grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %).
36920836	4	37	theme	Cytobacillus	440:451	arg1	FJAT-18043T					460:470	Cytobacillus solani FJAT-18043T	440:470	Cytobacillus solani FJAT-18043T (98.0 %)	440:479	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	4	37	theme	Cytobacillus	440:451	arg1	%					478:478	98.0 %	473:478	98.0 %	473:478	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	2	38	theme	citrus	169:174	arg1	sample					193:198	the citrus rhizosphere soil sample	165:198	the citrus rhizosphere soil sample	165:198	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	13	39	theme	=CCTCC AB	1344:1352	arg1	FJAT-49705T					1331:1341	FJAT-49705T	1331:1341	FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T)	1331:1374	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	13	39	theme	=CCTCC AB	1344:1352	arg1	31580T					1368:1373	=CCTCC AB 2019243T= LMG 31580T	1344:1373	=CCTCC AB 2019243T= LMG 31580T	1344:1373	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	11	40	theme	name	1264:1267	arg1	sp					1290:1291	the name Cytobacillus citreus sp	1260:1291	the name Cytobacillus citreus sp	1260:1291	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	2	41	attach	isolated	151:158	arg1	sample					193:198	the citrus rhizosphere soil sample	165:198	the citrus rhizosphere soil sample	165:198	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	2	41	attach	isolated	151:158	arg2	strain					115:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	11	42	theme	strain	1177:1182	arg1	FJAT-49705T					1184:1194	strain FJAT-49705T	1177:1194	strain FJAT-49705T	1177:1194	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	2	43	theme	rod-shaped	93:102	arg1	strain					115:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	4	44	theme	16S	357:359	arg1	similarity					380:389	high 16S rRNA gene sequence similarity	352:389	high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %)	352:479	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	13	45	theme	2019243T=	1354:1362	arg1	FJAT-49705T					1331:1341	FJAT-49705T	1331:1341	FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T)	1331:1374	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	13	45	theme	2019243T=	1354:1362	arg1	31580T					1368:1373	=CCTCC AB 2019243T= LMG 31580T	1344:1373	=CCTCC AB 2019243T= LMG 31580T	1344:1373	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	11	46	theme	above	1162:1166	arg1	results					1168:1174	the above results	1158:1174	the above results	1158:1174	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	10	47	theme	B.	998:999	arg1	FJAT-25496T					1012:1022	'B. dafuensis' FJAT-25496T	997:1022	'B. dafuensis' FJAT-25496T	997:1022	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	10	48	theme	average	932:938	arg1	ANI					961:963	ANI	961:963	ANI	961:963	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	10	48	theme	average	932:938	arg1	identity					951:958	The average nucleotide identity	928:958	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T	928:1048	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	4	49	theme	high	352:355	arg1	similarity					380:389	high 16S rRNA gene sequence similarity	352:389	high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %)	352:479	Strain FJAT-49705T showed high 16S rRNA gene sequence similarity to 'Bacillus dafuensis' FJAT-25496T (99.7 %) and Cytobacillus solani FJAT-18043T (98.0 %).
36920836	13	50	theme	LMG	1364:1366	arg1	FJAT-49705T					1331:1341	FJAT-49705T	1331:1341	FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T)	1331:1374	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	13	50	theme	LMG	1364:1366	arg1	31580T					1368:1373	=CCTCC AB 2019243T= LMG 31580T	1344:1373	=CCTCC AB 2019243T= LMG 31580T	1344:1373	The type strain is FJAT-49705T (=CCTCC AB 2019243T= LMG 31580T).
36920836	3	51	dep	optimum	238:244	arg1	°C					250:251	30 °C	247:251	30 °C	247:251	Strain FJAT-49705T grew at 20-40 °C (optimum, 30 °C) and pH 6.0-11.0 (optimum, pH 7.0) with 0-5 % (w/v) NaCl (optimum, 2 %).
36920836	2	52	theme	Gram-stain-positive	72:90	arg1	strain					115:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain	70:120	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	6	53	theme	only	685:688	arg1	MK-7					672:675	MK-7	672:675	MK-7	672:675	MK-7 was the only isoprenoid quinone present.
36920836	6	53	theme	only	685:688	arg1	present					709:715	the only isoprenoid quinone present	681:715	the only isoprenoid quinone present	681:715	MK-7 was the only isoprenoid quinone present.
36920836	6	54	theme	quinone	701:707	arg1	MK-7					672:675	MK-7	672:675	MK-7	672:675	MK-7 was the only isoprenoid quinone present.
36920836	6	54	theme	quinone	701:707	arg1	present					709:715	the only isoprenoid quinone present	681:715	the only isoprenoid quinone present	681:715	MK-7 was the only isoprenoid quinone present.
36920836	5	55	theme	phylogenomic	537:548	arg1	trees					550:554	phylogenomic trees	537:554	phylogenomic trees (based on 71 bacterial single-copy genes)	537:596	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36920836	10	56	theme	cut-off	1065:1071	arg1	level					1073:1077	the cut-off level	1061:1077	the cut-off level (95-96 %)	1061:1087	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	10	56	theme	cut-off	1065:1071	arg1	%					1086:1086	95-96 %	1080:1086	95-96 %	1080:1086	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	2	57	theme	soil	188:191	arg1	sample					193:198	the citrus rhizosphere soil sample	165:198	the citrus rhizosphere soil sample	165:198	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	10	58	theme	interspecies	1126:1137	arg1	identity					1139:1146	interspecies identity	1126:1146	interspecies identity	1126:1146	The average nucleotide identity (ANI) values between FJAT-49705T and 'B. dafuensis' FJAT-25496T and C. solani FJAT-18043T were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
36920836	2	59	theme	rhizosphere	176:186	arg1	sample					193:198	the citrus rhizosphere soil sample	165:198	the citrus rhizosphere soil sample	165:198	A Gram-stain-positive, rod-shaped and motile strain, designated FJAT-49705T, was isolated from the citrus rhizosphere soil sample.
36920836	9	60	theme	DNA	900:902	arg1	content					908:914	The genomic DNA G+C content	888:914	The genomic DNA G+C content	888:914	The genomic DNA G+C content was 36.9 %.
36920836	9	60	theme	DNA	900:902	arg1	%					925:925	36.9 %	920:925	36.9 %	920:925	The genomic DNA G+C content was 36.9 %.
36920836	9	61	theme	genomic	892:898	arg1	content					908:914	The genomic DNA G+C content	888:914	The genomic DNA G+C content	888:914	The genomic DNA G+C content was 36.9 %.
36920836	9	61	theme	genomic	892:898	arg1	%					925:925	36.9 %	920:925	36.9 %	920:925	The genomic DNA G+C content was 36.9 %.
36920836	11	62	theme	novel	1209:1213	arg1	species					1215:1221	a novel species	1207:1221	a novel species	1207:1221	Based on the above results, strain FJAT-49705T represents a novel species of the genus Cytobacillus, for which the name Cytobacillus citreus sp.
36920836	8	63	theme	fatty	838:842	arg1	anteiso-C15 					855:866	anteiso-C15 	855:866	anteiso-C15 	855:866	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
36920836	8	63	theme	fatty	838:842	arg1	acids					844:848	The major fatty acids	828:848	The major fatty acids	828:848	The major fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
36920836	7	64	theme	polar	727:731	arg1	diphosphatidylglycerol					745:766	diphosphatidylglycerol	745:766	diphosphatidylglycerol	745:766	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
36920836	7	64	theme	polar	727:731	arg1	lipids					733:738	The main polar lipids	718:738	The main polar lipids	718:738	The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
36920836	5	65	theme	single-copy	579:589	arg1	genes					591:595	71 bacterial single-copy genes	566:595	71 bacterial single-copy genes	566:595	In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic trees (based on 71 bacterial single-copy genes), strain FJAT-49705T clustered with the members of the genus Cytobacillus.
36839309	2	0	theme	effective	341:349	arg1	anti-inflammatories					351:369	effective anti-inflammatories	341:369	effective anti-inflammatories	341:369	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	0	1	theme	Gut	73:75	arg1	Microbiota					77:86	Remodeling Gut Microbiota	62:86	Remodeling Gut Microbiota	62:86	IRW (Ile-Arg-Trp) Alleviates DSS-Induced Intestinal Injury by Remodeling Gut Microbiota and Regulating Fecal SCFA Levels.
36839309	5	2	theme	Lactobacillus	912:924	arg1	abundance					885:893	the relative abundance	872:893	the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW	872:981	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	2	3	theme	biological	312:321	arg1	functions					323:331	biological functions	312:331	biological functions	312:331	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	0	4	theme	Regulating	92:101	arg1	Levels					114:119	Regulating Fecal SCFA Levels	92:119	Regulating Fecal SCFA Levels	92:119	IRW (Ile-Arg-Trp) Alleviates DSS-Induced Intestinal Injury by Remodeling Gut Microbiota and Regulating Fecal SCFA Levels.
36839309	2	5	theme	Dietary	277:283	arg1	peptides					285:292	Dietary peptides	277:292	Dietary peptides	277:292	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	4	6	theme	dextran	747:753	arg1	DSS					777:779	dextran sulphate sodium salt (DSS)	747:780	dextran sulphate sodium salt (DSS)	747:780	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	4	7	theme	salt	771:774	arg1	DSS					777:779	dextran sulphate sodium salt (DSS)	747:780	dextran sulphate sodium salt (DSS)	747:780	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	4	8	theme	mouse	708:712	arg1	damage					730:735	mouse colonic barrier damage	708:735	mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS)	708:780	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	1	9	theme	increasing	245:254	arg1	worldwide					266:274	an increasing incidence worldwide	242:274	an increasing incidence worldwide	242:274	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	0	10	theme	SCFA	109:112	arg1	Levels					114:119	Regulating Fecal SCFA Levels	92:119	Regulating Fecal SCFA Levels	92:119	IRW (Ile-Arg-Trp) Alleviates DSS-Induced Intestinal Injury by Remodeling Gut Microbiota and Regulating Fecal SCFA Levels.
36839309	5	11	theme	community	838:846	arg1	composition					848:858	microbial community composition	828:858	microbial community composition	828:858	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	5	12	theme	gut	933:935	arg1	microbiota					937:946	the gut microbiota	929:946	the gut microbiota	929:946	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	6	13	theme	Odoribacter	1105:1115	arg1	abundance					1092:1100	the relative abundance	1079:1100	the relative abundance of Odoribacter	1079:1115	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	0	14	theme	Fecal	103:107	arg1	Levels					114:119	Regulating Fecal SCFA Levels	92:119	Regulating Fecal SCFA Levels	92:119	IRW (Ile-Arg-Trp) Alleviates DSS-Induced Intestinal Injury by Remodeling Gut Microbiota and Regulating Fecal SCFA Levels.
36839309	6	15	theme	acetic	1199:1204	arg1	acid					1206:1209	acetic acid	1199:1209	acetic acid	1199:1209	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	2	16	theme	intestinal	446:455	arg1	inflammation					457:468	intestinal inflammation	446:468	intestinal inflammation	446:468	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	7	17	theme	Lactobacillus	1312:1324	arg1	abundance					1271:1279	the abundance	1267:1279	the abundance of Bacillota, Odoribacter, and Lactobacillus	1267:1324	IRW at 0.02 mg/mL and 0.04 mg/mL significantly altered the abundance of Bacillota, Odoribacter, and Lactobacillus.
36839309	7	18	theme	Bacillota	1284:1292	arg1	abundance					1271:1279	the abundance	1267:1279	the abundance of Bacillota, Odoribacter, and Lactobacillus	1267:1324	IRW at 0.02 mg/mL and 0.04 mg/mL significantly altered the abundance of Bacillota, Odoribacter, and Lactobacillus.
36839309	3	19	theme	intestinal	565:574	arg1	changes					586:592	intestinal microbial changes	565:592	intestinal microbial changes	565:592	Our study investigated the association between Ile-Arg-Trp (IRW), a dietary oligopeptide, and intestinal microbial changes during the relief of colitis using different concentrations of IRW.
36839309	7	20	theme	Odoribacter	1295:1305	arg1	abundance					1271:1279	the abundance	1267:1279	the abundance of Bacillota, Odoribacter, and Lactobacillus	1267:1324	IRW at 0.02 mg/mL and 0.04 mg/mL significantly altered the abundance of Bacillota, Odoribacter, and Lactobacillus.
36839309	1	21	theme	chronic	160:166	arg1	disease					168:174	a chronic disease	158:174	a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide	158:274	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	21	theme	chronic	160:166	arg1	disease					141:147	Inflammatory bowel disease	122:147	Inflammatory bowel disease (IBD)	122:153	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	22	theme	incidence	256:264	arg1	worldwide					266:274	an increasing incidence worldwide	242:274	an increasing incidence worldwide	242:274	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	3	23	theme	microbial	576:584	arg1	changes					586:592	intestinal microbial changes	565:592	intestinal microbial changes	565:592	Our study investigated the association between Ile-Arg-Trp (IRW), a dietary oligopeptide, and intestinal microbial changes during the relief of colitis using different concentrations of IRW.
36839309	5	24	from	abundance	885:893	arg1	microbiota					937:946	the gut microbiota	929:946	the gut microbiota	929:946	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	5	25	theme	composition	848:858	arg1	results					817:823	The results	813:823	The results of microbial community composition	813:858	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	4	26	theme	sodium	764:769	arg1	DSS					777:779	dextran sulphate sodium salt (DSS)	747:780	dextran sulphate sodium salt (DSS)	747:780	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	5	27	theme	IRW	979:981	arg1	concentrations					961:974	different concentrations	951:974	different concentrations of IRW	951:981	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	2	28	theme	material	424:431	arg1	class					415:419	a prospective class	401:419	a prospective class of material for treating intestinal inflammation	401:468	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	5	29	theme	different	951:959	arg1	concentrations					961:974	different concentrations	951:974	different concentrations of IRW	951:981	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	3	30	theme	IRW	657:659	arg1	concentrations					639:652	different concentrations	629:652	different concentrations of IRW	629:659	Our study investigated the association between Ile-Arg-Trp (IRW), a dietary oligopeptide, and intestinal microbial changes during the relief of colitis using different concentrations of IRW.
36839309	4	31	theme	sulphate	755:762	arg1	DSS					777:779	dextran sulphate sodium salt (DSS)	747:780	dextran sulphate sodium salt (DSS)	747:780	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	1	32	theme	unknown	179:185	arg1	etiology					187:194	unknown etiology	179:194	unknown etiology	179:194	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	0	33	theme	DSS-Induced	29:39	arg1	Injury					52:57	DSS-Induced Intestinal Injury	29:57	DSS-Induced Intestinal Injury	29:57	IRW (Ile-Arg-Trp) Alleviates DSS-Induced Intestinal Injury by Remodeling Gut Microbiota and Regulating Fecal SCFA Levels.
36839309	4	34	theme	colonic	714:720	arg1	damage					730:735	mouse colonic barrier damage	708:735	mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS)	708:780	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	2	35	theme	prospective	403:413	arg1	class					415:419	a prospective class	401:419	a prospective class of material for treating intestinal inflammation	401:468	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	6	36	theme	relative	1083:1090	arg1	abundance					1092:1100	the relative abundance	1079:1100	the relative abundance of Odoribacter	1079:1115	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	1	37	theme	etiology	187:194	arg1	disease					168:174	a chronic disease	158:174	a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide	158:274	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	37	theme	etiology	187:194	arg1	disease					141:147	Inflammatory bowel disease	122:147	Inflammatory bowel disease (IBD)	122:153	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	5	38	theme	relative	876:883	arg1	abundance					885:893	the relative abundance	872:893	the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW	872:981	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	7	39	from	mg/mL	1224:1228	arg1	mg/mL					1239:1243	0.04 mg/mL	1234:1243	0.04 mg/mL	1234:1243	IRW at 0.02 mg/mL and 0.04 mg/mL significantly altered the abundance of Bacillota, Odoribacter, and Lactobacillus.
36839309	7	39	from	mg/mL	1224:1228	arg1	IRW					1212:1214	IRW	1212:1214	IRW at 0.02 mg/mL	1212:1228	IRW at 0.02 mg/mL and 0.04 mg/mL significantly altered the abundance of Bacillota, Odoribacter, and Lactobacillus.
36839309	5	40	theme	Bacteroides	1037:1047	arg1	abundance					1024:1032	the abundance	1020:1032	the abundance of Bacteroides	1020:1047	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	6	41	with	correlation	1182:1192	arg1	acid					1206:1209	acetic acid	1199:1209	acetic acid	1199:1209	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	2	42	contain	have	294:297	arg2	variety					301:307	a variety	299:307	a variety of biological functions	299:331	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	2	42	contain	have	294:297	arg1	peptides					285:292	Dietary peptides	277:292	Dietary peptides	277:292	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	2	42	contain	have	294:297	arg2	functions					323:331	biological functions	312:331	biological functions	312:331	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	6	43	contain	had	1167:1169	arg1	abundance					1092:1100	the relative abundance	1079:1100	the relative abundance of Odoribacter	1079:1115	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	6	43	contain	had	1167:1169	arg2	correlation					1182:1192	a positive correlation	1171:1192	a positive correlation with acetic acid	1171:1209	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	4	44	theme	barrier	722:728	arg1	damage					730:735	mouse colonic barrier damage	708:735	mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS)	708:780	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	0	45	theme	Intestinal	41:50	arg1	Injury					52:57	DSS-Induced Intestinal Injury	29:57	DSS-Induced Intestinal Injury	29:57	IRW (Ile-Arg-Trp) Alleviates DSS-Induced Intestinal Injury by Remodeling Gut Microbiota and Regulating Fecal SCFA Levels.
36839309	1	46	with	disease	168:174	arg1	worldwide					266:274	an increasing incidence worldwide	242:274	an increasing incidence worldwide	242:274	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	46	with	disease	168:174	arg1	course					231:236	a progressive and destructive course	201:236	a progressive and destructive course	201:236	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	6	47	theme	positive	1173:1180	arg1	correlation					1182:1192	a positive correlation	1171:1192	a positive correlation with acetic acid	1171:1209	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	2	48	theme	functions	323:331	arg1	functions					323:331	biological functions	312:331	biological functions	312:331	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	2	48	theme	functions	323:331	arg1	variety					301:307	a variety	299:307	a variety of biological functions	299:331	Dietary peptides have a variety of biological functions and are effective anti-inflammatories and antioxidants, making them a prospective class of material for treating intestinal inflammation.
36839309	5	49	from	concentrations	961:974	arg1	abundance					885:893	the relative abundance	872:893	the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW	872:981	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	4	50	theme	intestinal	794:803	arg1	health					805:810	intestinal health	794:810	intestinal health	794:810	We found that IRW can significantly alleviate mouse colonic barrier damage caused by dextran sulphate sodium salt (DSS) and promote intestinal health.
36839309	1	51	theme	Inflammatory	122:133	arg1	disease					168:174	a chronic disease	158:174	a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide	158:274	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	51	theme	Inflammatory	122:133	arg1	IBD					150:152	IBD	150:152	IBD	150:152	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	51	theme	Inflammatory	122:133	arg1	disease					141:147	Inflammatory bowel disease	122:147	Inflammatory bowel disease (IBD)	122:153	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	6	52	theme	IRW	1145:1147	arg1	concentration					1149:1161	IRW concentration	1145:1161	IRW concentration	1145:1161	Surprisingly, the relative abundance of Odoribacter also received regulation by IRW concentration and had a positive correlation with acetic acid.
36839309	3	53	theme	different	629:637	arg1	concentrations					639:652	different concentrations	629:652	different concentrations of IRW	629:659	Our study investigated the association between Ile-Arg-Trp (IRW), a dietary oligopeptide, and intestinal microbial changes during the relief of colitis using different concentrations of IRW.
36839309	1	54	theme	bowel	135:139	arg1	disease					168:174	a chronic disease	158:174	a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide	158:274	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	54	theme	bowel	135:139	arg1	IBD					150:152	IBD	150:152	IBD	150:152	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	54	theme	bowel	135:139	arg1	disease					141:147	Inflammatory bowel disease	122:147	Inflammatory bowel disease (IBD)	122:153	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	1	55	theme	progressive	203:213	arg1	course					231:236	a progressive and destructive course	201:236	a progressive and destructive course	201:236	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
36839309	0	56	theme	Remodeling	62:71	arg1	Microbiota					77:86	Remodeling Gut Microbiota	62:86	Remodeling Gut Microbiota	62:86	IRW (Ile-Arg-Trp) Alleviates DSS-Induced Intestinal Injury by Remodeling Gut Microbiota and Regulating Fecal SCFA Levels.
36839309	5	57	theme	microbial	828:836	arg1	composition					848:858	microbial community composition	828:858	microbial community composition	828:858	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	3	58	theme	dietary	539:545	arg1	oligopeptide					547:558	a dietary oligopeptide	537:558	a dietary oligopeptide	537:558	Our study investigated the association between Ile-Arg-Trp (IRW), a dietary oligopeptide, and intestinal microbial changes during the relief of colitis using different concentrations of IRW.
36839309	3	59	theme	colitis	615:621	arg1	relief					605:610	the relief	601:610	the relief of colitis	601:621	Our study investigated the association between Ile-Arg-Trp (IRW), a dietary oligopeptide, and intestinal microbial changes during the relief of colitis using different concentrations of IRW.
36839309	5	60	theme	Bacillota	898:906	arg1	abundance					885:893	the relative abundance	872:893	the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW	872:981	The results of microbial community composition showed that the relative abundance of Bacillota and Lactobacillus in the gut microbiota at different concentrations of IRW was significantly increased and that the abundance of Bacteroides was suppressed.
36839309	1	61	theme	destructive	219:229	arg1	course					231:236	a progressive and destructive course	201:236	a progressive and destructive course	201:236	Inflammatory bowel disease (IBD) is a chronic disease of unknown etiology with a progressive and destructive course and an increasing incidence worldwide.
35524685	8	0	theme	microbial	1588:1596	arg1	composition					1598:1608	altered gut microbial composition	1576:1608	altered gut microbial composition	1576:1608	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	7	1	dep	increased	1163:1171	arg1	<					1193:1193	P < 0.05	1191:1198	P < 0.05	1191:1198	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	2	2	theme	typical	300:306	arg1	vegetable					322:330	a typical Mediterranean vegetable	298:330	a typical Mediterranean vegetable	298:330	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	2	2	theme	typical	300:306	arg1	chicory					289:295	Brussels chicory	280:295	Brussels chicory	280:295	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	8	3	theme	members	1675:1681	arg1	abundance					1654:1662	increased relative abundance	1635:1662	increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05)	1635:1792	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	4	dep	IL1β	1468:1471	arg1	%					1487:1487	52.0% and 33.8%	1483:1497	%	1487:1487	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	4	dep	IL1β	1468:1471	arg1	%					1497:1497	52.0% and 33.8%	1483:1497	%	1497:1497	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	7	5	theme	P	1191:1191	arg1	<					1193:1193	P < 0.05	1191:1198	P < 0.05	1191:1198	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	0	6	theme	Brussels	0:7	arg1	Chicory					9:15	Brussels Chicory	0:15	Brussels Chicory	0:15	Brussels Chicory Stabilizes Unstable Atherosclerotic Plaques and Reshapes the Gut Microbiota in Apoe-/- Mice.
35524685	5	7	theme	inflammation-related	934:953	arg1	cytokines					955:963	inflammation-related cytokines	934:963	inflammation-related cytokines	934:963	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	2	8	from	formation	363:371	arg1	mice					407:410	mice	407:410	mice	407:410	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	9	9	theme	permeability	1914:1925	arg1	LPS					1944:1946	intestinal permeability, fecal and serum LPS	1903:1946	intestinal permeability, fecal and serum LPS	1903:1946	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	4	10	theme	%	750:750	arg1	chicory					774:780	0.5% freeze-dried Brussels chicory	747:780	0.5% freeze-dried Brussels chicory	747:780	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	8	11	theme	family	1706:1711	arg1	Intestinimonas					1768:1781	Intestinimonas	1768:1781	Intestinimonas (P < 0.05)	1768:1792	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	11	theme	family	1706:1711	arg1	members					1675:1681	certain members	1667:1681	certain members of the Ruminococcaceae family	1667:1711	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	11	theme	family	1706:1711	arg1	such					1714:1717	such	1714:1717	such	1714:1717	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	6	12	theme	Brussels	1053:1060	arg1	consumption					1070:1080	Brussels chicory consumption	1053:1080	Brussels chicory consumption	1053:1080	RESULTS Compared with the control treatment, Brussels chicory consumption did not significantly change plaque volume and serum lipid profiles.
35524685	9	13	theme	fecal	1928:1932	arg1	LPS					1944:1946	intestinal permeability, fecal and serum LPS	1903:1946	intestinal permeability, fecal and serum LPS	1903:1946	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	5	14	theme	serum	909:913	arg1	profiles					921:928	serum lipid profiles	909:928	serum lipid profiles	909:928	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	0	15	from	Microbiota	82:91	arg1	Mice					104:107	Apoe-/- Mice	96:107	Apoe-/- Mice	96:107	Brussels Chicory Stabilizes Unstable Atherosclerotic Plaques and Reshapes the Gut Microbiota in Apoe-/- Mice.
35524685	9	16	theme	serum	1938:1942	arg1	LPS					1944:1946	intestinal permeability, fecal and serum LPS	1903:1946	intestinal permeability, fecal and serum LPS	1903:1946	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	5	17	theme	Plaque	793:798	arg1	volume					800:805	Plaque volume	793:805	Plaque volume	793:805	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	0	18	theme	Unstable	28:35	arg1	Plaques					53:59	Unstable Atherosclerotic Plaques	28:59	Unstable Atherosclerotic Plaques	28:59	Brussels Chicory Stabilizes Unstable Atherosclerotic Plaques and Reshapes the Gut Microbiota in Apoe-/- Mice.
35524685	5	19	theme	LPS	889:891	arg1	concentrations					893:906	fecal and serum LPS concentrations	873:906	concentrations	893:906	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	4	20	dep	alone	710:714	arg1	control					717:723	control	717:723	control	717:723	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	9	21	theme	proinflammatory	1955:1969	arg1	cytokines					1971:1979	serum proinflammatory cytokines	1949:1979	serum proinflammatory cytokines	1949:1979	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	2	22	theme	Brussels	280:287	arg1	vegetable					322:330	a typical Mediterranean vegetable	298:330	a typical Mediterranean vegetable	298:330	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	2	22	theme	Brussels	280:287	arg1	chicory					289:295	Brussels chicory	280:295	Brussels chicory	280:295	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	1	23	theme	Mediterranean	136:148	arg1	pattern					158:164	a Mediterranean dietary pattern	134:164	a Mediterranean dietary pattern	134:164	BACKGROUND Adherence to a Mediterranean dietary pattern can protect against atherosclerosis in part by reducing intestinal permeability and gut microbial LPS production.
35524685	4	24	theme	Brussels	765:772	arg1	chicory					774:780	0.5% freeze-dried Brussels chicory	747:780	0.5% freeze-dried Brussels chicory	747:780	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	7	25	theme	cap	1276:1278	arg1	thickness					1280:1288	fibrous cap thickness	1268:1288	fibrous cap thickness (55.0%)	1268:1296	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	7	25	theme	cap	1276:1278	arg1	%					1295:1295	55.0%	1291:1295	55.0%	1291:1295	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	5	26	theme	gut	970:972	arg1	profiles					984:991	gut microbial profiles	970:991	gut microbial profiles	970:991	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	11	27	theme	slow	2220:2223	arg1	progression					2229:2239	slow the progression	2220:2239	slow the progression of atherosclerosis	2220:2258	This study provides a promising approach to slow the progression of atherosclerosis.
35524685	9	28	theme	several	1986:1992	arg1	features					1994:2001	several features	1986:2001	several features of plaque stability	1986:2021	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	8	29	theme	Brussels	1338:1345	arg1	consumption					1355:1365	Brussels chicory consumption	1338:1365	Brussels chicory consumption	1338:1365	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	5	30	theme	plaque	841:846	arg1	polarization					859:870	plaque macrophage polarization	841:870	plaque macrophage polarization	841:870	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	9	31	theme	Spearman	1795:1802	arg1	analyses					1816:1823	Spearman correlation analyses	1795:1823	Spearman correlation analyses	1795:1823	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	10	32	theme	CONCLUSIONS	2024:2034	arg1	chicory					2045:2051	CONCLUSIONS Brussels chicory	2024:2051	CONCLUSIONS Brussels chicory	2024:2051	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	0	33	theme	Apoe-/-	96:102	arg1	Mice					104:107	Apoe-/- Mice	96:107	Apoe-/- Mice	96:107	Brussels Chicory Stabilizes Unstable Atherosclerotic Plaques and Reshapes the Gut Microbiota in Apoe-/- Mice.
35524685	11	34	theme	atherosclerosis	2244:2258	arg1	progression					2229:2239	slow the progression	2220:2239	slow the progression of atherosclerosis	2220:2258	This study provides a promising approach to slow the progression of atherosclerosis.
35524685	9	35	theme	stability	2013:2021	arg1	LPS					1944:1946	intestinal permeability, fecal and serum LPS	1903:1946	intestinal permeability, fecal and serum LPS	1903:1946	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	9	35	theme	stability	2013:2021	arg1	features					1994:2001	several features	1986:2001	several features of plaque stability	1986:2021	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	9	35	theme	stability	2013:2021	arg1	cytokines					1971:1979	serum proinflammatory cytokines	1949:1979	serum proinflammatory cytokines	1949:1979	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	8	36	theme	intestinal	1375:1384	arg1	permeability					1386:1397	intestinal permeability	1375:1397	intestinal permeability (56.3%)	1375:1405	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	36	theme	intestinal	1375:1384	arg1	%					1404:1404	56.3%	1400:1404	56.3%	1400:1404	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	3	37	theme	advanced	470:477	arg1	progression					495:505	advanced atherosclerosis progression	470:505	advanced atherosclerosis progression	470:505	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	7	38	theme	fibrous	1268:1274	arg1	thickness					1280:1288	fibrous cap thickness	1268:1288	fibrous cap thickness (55.0%)	1268:1296	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	7	38	theme	fibrous	1268:1274	arg1	%					1295:1295	55.0%	1291:1295	55.0%	1291:1295	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	5	39	theme	serum	883:887	arg1	concentrations					893:906	fecal and serum LPS concentrations	873:906	concentrations	893:906	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	3	40	theme	intestinal	508:517	arg1	permeability					519:530	intestinal permeability	508:530	intestinal permeability	508:530	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	7	41	theme	core	1235:1238	arg1	size					1240:1243	reduced necrotic core size	1218:1243	reduced necrotic core size (42.3%)	1218:1251	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	7	41	theme	core	1235:1238	arg1	%					1250:1250	42.3%	1246:1250	42.3%	1246:1250	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	1	42	theme	BACKGROUND	110:119	arg1	Adherence					121:129	BACKGROUND Adherence	110:129	BACKGROUND Adherence to a Mediterranean dietary pattern	110:164	BACKGROUND Adherence to a Mediterranean dietary pattern can protect against atherosclerosis in part by reducing intestinal permeability and gut microbial LPS production.
35524685	4	43	theme	atherosclerotic	630:644	arg1	plaques					646:652	unstable atherosclerotic plaques	621:652	unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk	621:790	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	7	44	theme	reduced	1218:1224	arg1	size					1240:1243	reduced necrotic core size	1218:1243	reduced necrotic core size (42.3%)	1218:1251	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	7	44	theme	reduced	1218:1224	arg1	%					1250:1250	42.3%	1246:1250	42.3%	1246:1250	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	4	45	dep	METHODS	567:573	arg1	mice					611:614	Thirty-week-old male apoE-deficient mice	575:614	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.	567:791	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	0	46	theme	Atherosclerotic	37:51	arg1	Plaques					53:59	Unstable Atherosclerotic Plaques	28:59	Unstable Atherosclerotic Plaques	28:59	Brussels Chicory Stabilizes Unstable Atherosclerotic Plaques and Reshapes the Gut Microbiota in Apoe-/- Mice.
35524685	6	47	theme	serum	1129:1133	arg1	profiles					1141:1148	serum lipid profiles	1129:1148	serum lipid profiles	1129:1148	RESULTS Compared with the control treatment, Brussels chicory consumption did not significantly change plaque volume and serum lipid profiles.
35524685	9	48	theme	correlation	1804:1814	arg1	analyses					1816:1823	Spearman correlation analyses	1795:1823	Spearman correlation analyses	1795:1823	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	8	49	theme	plaque macrophage	1510:1526	arg1	polarization					1528:1539	promoted plaque macrophage polarization	1501:1539	promoted plaque macrophage polarization towards the M2-like phenotype	1501:1569	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	5	50	theme	stability	830:838	arg1	profiles					921:928	serum lipid profiles	909:928	serum lipid profiles	909:928	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	5	50	theme	stability	830:838	arg1	volume					800:805	Plaque volume	793:805	Plaque volume	793:805	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	5	50	theme	stability	830:838	arg1	profiles					984:991	gut microbial profiles	970:991	gut microbial profiles	970:991	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	5	50	theme	stability	830:838	arg1	concentrations					893:906	fecal and serum LPS concentrations	873:906	concentrations	893:906	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	5	50	theme	stability	830:838	arg1	features					811:818	features	811:818	features	811:818	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	5	50	theme	stability	830:838	arg1	cytokines					955:963	inflammation-related cytokines	934:963	inflammation-related cytokines	934:963	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	5	50	theme	stability	830:838	arg1	polarization					859:870	plaque macrophage polarization	841:870	plaque macrophage polarization	841:870	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	3	51	theme	gut	537:539	arg1	production					555:564	gut microbial LPS production	537:564	gut microbial LPS production	537:564	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	5	52	theme	fecal	873:877	arg1	concentrations					893:906	fecal and serum LPS concentrations	873:906	concentrations	893:906	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	10	53	theme	atherosclerotic	2074:2088	arg1	plaques					2090:2096	atherosclerotic plaques	2074:2096	atherosclerotic plaques in mice	2074:2104	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	4	54	theme	apoE-deficient	596:609	arg1	mice					611:614	Thirty-week-old male apoE-deficient mice	575:614	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.	567:791	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	8	55	theme	LPS	1424:1426	arg1	concentrations					1428:1441	serum LPS concentrations	1418:1441	serum LPS concentrations	1418:1441	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	56	dep	fecal	1408:1412	arg1	%					1448:1448	52.2% and 39.4%	1444:1458	%	1448:1448	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	56	dep	fecal	1408:1412	arg1	%					1458:1458	52.2% and 39.4%	1444:1458	%	1458:1458	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	10	57	theme	intestinal	2118:2127	arg1	permeability					2129:2140	intestinal permeability	2118:2140	intestinal permeability	2118:2140	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	2	58	theme	atherosclerosis	388:402	arg1	formation					363:371	the formation	359:371	the formation of early-stage atherosclerosis in mice	359:410	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	1	59	theme	microbial	254:262	arg1	production					268:277	gut microbial LPS production	250:277	gut microbial LPS production	250:277	BACKGROUND Adherence to a Mediterranean dietary pattern can protect against atherosclerosis in part by reducing intestinal permeability and gut microbial LPS production.
35524685	9	60	theme	bacterial	1851:1859	arg1	genera					1861:1866	these bacterial genera	1845:1866	these bacterial genera	1845:1866	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	4	61	theme	Thirty-week-old	575:589	arg1	mice					611:614	Thirty-week-old male apoE-deficient mice	575:614	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.	567:791	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	3	62	theme	LPS	551:553	arg1	production					555:564	gut microbial LPS production	537:564	gut microbial LPS production	537:564	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	8	63	theme	increased	1635:1643	arg1	abundance					1654:1662	increased relative abundance	1635:1662	increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05)	1635:1792	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	64	theme	gut	1584:1586	arg1	composition					1598:1608	altered gut microbial composition	1576:1608	altered gut microbial composition	1576:1608	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	2	65	theme	Mediterranean	308:320	arg1	vegetable					322:330	a typical Mediterranean vegetable	298:330	a typical Mediterranean vegetable	298:330	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	2	65	theme	Mediterranean	308:320	arg1	chicory					289:295	Brussels chicory	280:295	Brussels chicory	280:295	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	8	66	theme	certain	1667:1673	arg1	Intestinimonas					1768:1781	Intestinimonas	1768:1781	Intestinimonas (P < 0.05)	1768:1792	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	66	theme	certain	1667:1673	arg1	members					1675:1681	certain members	1667:1681	certain members of the Ruminococcaceae family	1667:1711	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	66	theme	certain	1667:1673	arg1	such					1714:1717	such	1714:1717	such	1714:1717	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	9	67	theme	intestinal	1903:1912	arg1	permeability					1914:1925	intestinal permeability	1903:1925	intestinal permeability	1903:1925	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	7	68	theme	plaque	1173:1178	arg1	stability					1180:1188	plaque stability	1173:1188	plaque stability	1173:1188	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	4	69	with	mice	611:614	arg1	plaques					646:652	unstable atherosclerotic plaques	621:652	unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk	621:790	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	6	70	theme	chicory	1062:1068	arg1	consumption					1070:1080	Brussels chicory consumption	1053:1080	Brussels chicory consumption	1053:1080	RESULTS Compared with the control treatment, Brussels chicory consumption did not significantly change plaque volume and serum lipid profiles.
35524685	4	71	theme	0.5	747:749	arg1	%					750:750	%	750:750	%	750:750	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	8	72	theme	Ruminococcaceae	1690:1704	arg1	family					1706:1711	the Ruminococcaceae family	1686:1711	the Ruminococcaceae family	1686:1711	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	10	73	theme	microbial	2150:2158	arg1	production					2164:2173	gut microbial LPS production	2146:2173	gut microbial LPS production	2146:2173	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	0	74	theme	Gut	78:80	arg1	Microbiota					82:91	the Gut Microbiota	74:91	the Gut Microbiota in Apoe-/- Mice	74:107	Brussels Chicory Stabilizes Unstable Atherosclerotic Plaques and Reshapes the Gut Microbiota in Apoe-/- Mice.
35524685	4	75	dep	brachiocephalic artery were	661:687	arg1	fed					689:691	fed	689:691	fed the AIN-93G diet alone (control)	689:724	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	4	75	dep	brachiocephalic artery were	661:687	arg1	supplemented					729:740	supplemented	729:740	supplemented with 0.5% freeze-dried Brussels chicory for 20 wk	729:790	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	5	76	theme	lipid	915:919	arg1	profiles					921:928	serum lipid profiles	909:928	serum lipid profiles	909:928	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	6	77	theme	control	1034:1040	arg1	treatment					1042:1050	the control treatment	1030:1050	the control treatment	1030:1050	RESULTS Compared with the control treatment, Brussels chicory consumption did not significantly change plaque volume and serum lipid profiles.
35524685	9	78	theme	serum	1949:1953	arg1	cytokines					1971:1979	serum proinflammatory cytokines	1949:1979	serum proinflammatory cytokines	1949:1979	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	8	79	theme	serum	1462:1466	arg1	IL1β					1468:1471	serum IL1β	1462:1471	serum IL1β	1462:1471	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	10	80	theme	LPS	2160:2162	arg1	production					2164:2173	gut microbial LPS production	2146:2173	gut microbial LPS production	2146:2173	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	5	81	theme	plaque	823:828	arg1	stability					830:838	plaque stability	823:838	plaque stability	823:838	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	1	82	theme	dietary	150:156	arg1	pattern					158:164	a Mediterranean dietary pattern	134:164	a Mediterranean dietary pattern	134:164	BACKGROUND Adherence to a Mediterranean dietary pattern can protect against atherosclerosis in part by reducing intestinal permeability and gut microbial LPS production.
35524685	6	83	theme	plaque	1111:1116	arg1	volume					1118:1123	plaque volume	1111:1123	plaque volume	1111:1123	RESULTS Compared with the control treatment, Brussels chicory consumption did not significantly change plaque volume and serum lipid profiles.
35524685	4	84	theme	freeze-dried	752:763	arg1	chicory					774:780	0.5% freeze-dried Brussels chicory	747:780	0.5% freeze-dried Brussels chicory	747:780	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	5	85	theme	microbial	974:982	arg1	profiles					984:991	gut microbial profiles	970:991	gut microbial profiles	970:991	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	10	86	theme	Brussels	2036:2043	arg1	chicory					2045:2051	CONCLUSIONS Brussels chicory	2024:2051	CONCLUSIONS Brussels chicory	2024:2051	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	8	87	theme	P	1784:1784	arg1	Intestinimonas					1768:1781	Intestinimonas	1768:1781	Intestinimonas (P < 0.05)	1768:1792	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	8	87	theme	P	1784:1784	arg1	<					1786:1786	P < 0.05	1784:1791	P < 0.05	1784:1791	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	3	88	dep	OBJECTIVES	413:422	arg1	evaluated					427:435	evaluated	427:435	evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production	427:564	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	9	89	theme	plaque	2006:2011	arg1	stability					2013:2021	plaque stability	2006:2021	plaque stability	2006:2021	Spearman correlation analyses further showed that these bacterial genera were significantly correlated with intestinal permeability, fecal and serum LPS, serum proinflammatory cytokines, and several features of plaque stability.
35524685	8	90	theme	chicory	1347:1353	arg1	consumption					1355:1365	Brussels chicory consumption	1338:1365	Brussels chicory consumption	1338:1365	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	3	91	theme	atherosclerosis	479:493	arg1	progression					495:505	advanced atherosclerosis progression	470:505	advanced atherosclerosis progression	470:505	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	4	92	from	plaques	646:652	arg1	brachiocephalic artery were					661:687	the brachiocephalic artery were	657:687	the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk	657:790	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	4	93	theme	unstable	621:628	arg1	plaques					646:652	unstable atherosclerotic plaques	621:652	unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk	621:790	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	10	94	from	plaques	2090:2096	arg1	mice					2101:2104	mice	2101:2104	mice	2101:2104	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	7	95	theme	necrotic	1226:1233	arg1	size					1240:1243	reduced necrotic core size	1218:1243	reduced necrotic core size (42.3%)	1218:1251	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	7	95	theme	necrotic	1226:1233	arg1	%					1250:1250	42.3%	1246:1250	42.3%	1246:1250	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	8	96	theme	promoted	1501:1508	arg1	polarization					1528:1539	promoted plaque macrophage polarization	1501:1539	promoted plaque macrophage polarization towards the M2-like phenotype	1501:1569	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	10	97	theme	gut	2146:2148	arg1	production					2164:2173	gut microbial LPS production	2146:2173	gut microbial LPS production	2146:2173	CONCLUSIONS Brussels chicory might help stabilize atherosclerotic plaques in mice by reducing intestinal permeability and gut microbial LPS production.
35524685	5	98	theme	macrophage	848:857	arg1	polarization					859:870	plaque macrophage polarization	841:870	plaque macrophage polarization	841:870	Plaque volume and features of plaque stability, plaque macrophage polarization, fecal and serum LPS concentrations, serum lipid profiles and inflammation-related cytokines, and gut microbial profiles were measured.
35524685	1	99	theme	intestinal	222:231	arg1	permeability					233:244	intestinal permeability	222:244	intestinal permeability	222:244	BACKGROUND Adherence to a Mediterranean dietary pattern can protect against atherosclerosis in part by reducing intestinal permeability and gut microbial LPS production.
35524685	6	100	theme	lipid	1135:1139	arg1	profiles					1141:1148	serum lipid profiles	1129:1148	serum lipid profiles	1129:1148	RESULTS Compared with the control treatment, Brussels chicory consumption did not significantly change plaque volume and serum lipid profiles.
35524685	3	101	theme	Brussels	445:452	arg1	chicory					454:460	Brussels chicory	445:460	Brussels chicory	445:460	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	4	102	theme	male	591:594	arg1	mice					611:614	Thirty-week-old male apoE-deficient mice	575:614	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.	567:791	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	7	103	theme	collagen	1302:1309	arg1	%					1324:1324	68.4%	1320:1324	68.4%	1320:1324	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	7	103	theme	collagen	1302:1309	arg1	content					1311:1317	collagen content	1302:1317	collagen content (68.4%)	1302:1325	However, it increased plaque stability (P < 0.05), as evidenced by reduced necrotic core size (42.3%), and increased fibrous cap thickness (55.0%) and collagen content (68.4%).
35524685	11	104	theme	promising	2198:2206	arg1	approach					2208:2215	a promising approach	2196:2215	a promising approach to slow the progression of atherosclerosis	2196:2258	This study provides a promising approach to slow the progression of atherosclerosis.
35524685	3	105	theme	microbial	541:549	arg1	production					555:564	gut microbial LPS production	537:564	gut microbial LPS production	537:564	OBJECTIVES We evaluated whether Brussels chicory affects advanced atherosclerosis progression, intestinal permeability, and gut microbial LPS production.
35524685	4	106	theme	AIN-93G	697:703	arg1	diet					705:708	the AIN-93G diet	693:708	the AIN-93G diet	693:708	METHODS Thirty-week-old male apoE-deficient mice with unstable atherosclerotic plaques in the brachiocephalic artery were fed the AIN-93G diet alone (control) or supplemented with 0.5% freeze-dried Brussels chicory for 20 wk.
35524685	8	107	theme	serum	1418:1422	arg1	concentrations					1428:1441	serum LPS concentrations	1418:1441	serum LPS concentrations	1418:1441	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	1	108	theme	gut	250:252	arg1	production					268:277	gut microbial LPS production	250:277	gut microbial LPS production	250:277	BACKGROUND Adherence to a Mediterranean dietary pattern can protect against atherosclerosis in part by reducing intestinal permeability and gut microbial LPS production.
35524685	8	109	theme	altered	1576:1582	arg1	composition					1598:1608	altered gut microbial composition	1576:1608	altered gut microbial composition	1576:1608	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	2	110	theme	early-stage	376:386	arg1	atherosclerosis					388:402	early-stage atherosclerosis	376:402	early-stage atherosclerosis	376:402	Brussels chicory, a typical Mediterranean vegetable, has been shown to inhibit the formation of early-stage atherosclerosis in mice.
35524685	8	111	theme	relative	1645:1652	arg1	abundance					1654:1662	increased relative abundance	1635:1662	increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05)	1635:1792	Moreover, Brussels chicory consumption reduced intestinal permeability (56.3%), fecal and serum LPS concentrations (52.2% and 39.4%), serum IL1β and TNFα (52.0% and 33.8%), promoted plaque macrophage polarization towards the M2-like phenotype, and altered gut microbial composition, the latter indicated by increased relative abundance of certain members of the Ruminococcaceae family, such as Ruminiclostridium_9, Ruminiclostridium_5, and Intestinimonas (P < 0.05).
35524685	1	112	theme	LPS	264:266	arg1	production					268:277	gut microbial LPS production	250:277	gut microbial LPS production	250:277	BACKGROUND Adherence to a Mediterranean dietary pattern can protect against atherosclerosis in part by reducing intestinal permeability and gut microbial LPS production.
36974935	4	0	dep	difficult	514:522	arg1	probe					536:540	probe	536:540	to probe due to their isomeric nature	533:569	Sugars, however, are difficult analytes to probe due to their isomeric nature.
36974935	5	1	theme	enhanced	617:624	arg1	SERS					646:649	SERS	646:649	SERS	646:649	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	5	1	theme	enhanced	617:624	arg1	spectroscopy					632:643	surface enhanced Raman spectroscopy	609:643	surface enhanced Raman spectroscopy (SERS)	609:650	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	4	2	theme	isomeric	555:562	arg1	nature					564:569	their isomeric nature	549:569	their isomeric nature	549:569	Sugars, however, are difficult analytes to probe due to their isomeric nature.
36974935	0	3	theme	Raman	84:88	arg1	spectroscopy					90:101	Raman and surface enhanced Raman spectroscopy	57:101	Raman and surface enhanced Raman spectroscopy	57:101	Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
36974935	6	4	theme	scattering	882:891	arg1	section					899:905	a larger Raman scattering cross section	867:905	a larger Raman scattering cross section	867:905	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	5	5	theme	Raman	626:630	arg1	SERS					646:649	SERS	646:649	SERS	646:649	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	5	5	theme	Raman	626:630	arg1	spectroscopy					632:643	surface enhanced Raman spectroscopy	609:643	surface enhanced Raman spectroscopy (SERS)	609:650	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	7	6	theme	SERS	1055:1058	arg1	detection					1060:1068	static SERS detection	1048:1068	static SERS detection	1048:1068	Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.
36974935	7	7	from	Raman	1023:1027	arg1	flow					1038:1041	flow	1038:1041	flow	1038:1041	Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.
36974935	6	8	theme	Raman	876:880	arg1	scattering					882:891	a larger Raman scattering	867:891	a larger Raman scattering cross section	867:905	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	6	9	theme	larger	869:874	arg1	scattering					882:891	a larger Raman scattering	867:891	a larger Raman scattering cross section	867:905	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	2	10	theme	common	310:315	arg1	modification					336:347	the most common post translational modification	301:347	the most common post translational modification	301:347	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	2	10	theme	common	310:315	arg1	Glycosylation					235:247	Glycosylation	235:247	Glycosylation	235:247	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	5	11	theme	Raman	586:590	arg1	spectroscopy					592:603	Raman spectroscopy	586:603	Raman spectroscopy	586:603	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	6	12	theme	weak	769:772	arg1	scattering					774:783	The weak scattering	765:783	The weak scattering of the sugars	765:797	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	0	13	theme	Raman	57:61	arg1	spectroscopy					90:101	Raman and surface enhanced Raman spectroscopy	57:101	Raman and surface enhanced Raman spectroscopy	57:101	Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
36974935	2	14	theme	residue	276:282	arg1	Glycosylation					235:247	Glycosylation	235:247	Glycosylation	235:247	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	2	14	theme	residue	276:282	arg1	attachment					254:263	the attachment	250:263	the attachment of a sugar residue to a protein	250:295	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	6	15	with	conjugation	820:830	arg1	acid					851:854	phenylboronic acid	837:854	phenylboronic acid	837:854	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	5	16	used	used	656:659	arg2	SERS					646:649	SERS	646:649	SERS	646:649	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	5	16	used	used	656:659	arg2	spectroscopy					632:643	surface enhanced Raman spectroscopy	609:643	surface enhanced Raman spectroscopy (SERS)	609:650	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	5	16	used	used	656:659	arg2	spectroscopy					592:603	Raman spectroscopy	586:603	Raman spectroscopy	586:603	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	3	17	dep	tool	399:402	arg1	development					428:438	pharmaceutical development	413:438	pharmaceutical development	413:438	Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further.
36974935	3	17	dep	tool	399:402	arg1	probing					451:457	probing	451:457	probing the proteome and glycome further	451:490	Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further.
36974935	5	18	theme	vibrational	719:729	arg1	modes					731:735	the vibrational modes	715:735	the vibrational modes of these isomeric analytes	715:762	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	1	19	theme	modifying	191:199	arg1	proteins					201:208	modifying proteins	191:208	modifying proteins	191:208	Sugars play important roles in numerous biological processes, from providing energy to modifying proteins to alter their function.
36974935	0	20	theme	Chemical	0:7	arg1	conjugation					9:19	Chemical conjugation	0:19	Chemical conjugation	0:19	Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
36974935	5	21	theme	surface	609:615	arg1	SERS					646:649	SERS	646:649	SERS	646:649	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	5	21	theme	surface	609:615	arg1	spectroscopy					632:643	surface enhanced Raman spectroscopy	609:643	surface enhanced Raman spectroscopy (SERS)	609:650	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	7	22	theme	static	1048:1053	arg1	detection					1060:1068	static SERS detection	1048:1068	static SERS detection	1048:1068	Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.
36974935	3	23	theme	pharmaceutical	413:426	arg1	development					428:438	pharmaceutical development	413:438	pharmaceutical development	413:438	Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further.
36974935	6	24	theme	observed	940:947	arg1	spectra					949:955	the observed spectra	936:955	the observed spectra associated with the structure of the monosaccharides	936:1008	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	0	25	dep	enhanced	75:82	arg1	surface					67:73	surface	67:73	surface	67:73	Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
36974935	7	26	from	detection	1060:1068	arg1	flow					1038:1041	flow	1038:1041	flow	1038:1041	Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.
36974935	1	27	theme	important	116:124	arg1	roles					126:130	important roles	116:130	important roles	116:130	Sugars play important roles in numerous biological processes, from providing energy to modifying proteins to alter their function.
36974935	6	28	theme	cross	893:897	arg1	section					899:905	a larger Raman scattering cross section	867:905	a larger Raman scattering cross section	867:905	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	7	29	theme	sugar	1256:1260	arg1	conjugates					1262:1271	these sugar conjugates	1250:1271	these sugar conjugates	1250:1271	Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.
36974935	5	30	theme	isomeric	746:753	arg1	analytes					755:762	these isomeric analytes	740:762	these isomeric analytes	740:762	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	5	31	theme	different	673:681	arg1	species					698:704	different monosaccharide species	673:704	different monosaccharide species based on the vibrational modes of these isomeric analytes	673:762	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	6	32	theme	phenylboronic	837:849	arg1	acid					851:854	phenylboronic acid	837:854	phenylboronic acid	837:854	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	5	33	theme	analytes	755:762	arg1	modes					731:735	the vibrational modes	715:735	the vibrational modes of these isomeric analytes	715:762	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	5	34	theme	monosaccharide	683:696	arg1	species					698:704	different monosaccharide species	673:704	different monosaccharide species based on the vibrational modes of these isomeric analytes	673:762	In this work, Raman spectroscopy and surface enhanced Raman spectroscopy (SERS) are used to identify different monosaccharide species based on the vibrational modes of these isomeric analytes.
36974935	6	35	theme	slight	918:923	arg1	changes					925:931	slight changes	918:931	slight changes in the observed spectra associated with the structure of the monosaccharides	918:1008	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	3	36	from	glycans	366:372	arg1	protein					379:385	a protein	377:385	a protein	377:385	Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further.
36974935	3	37	dep	development	428:438	arg1	both					404:407	both	404:407	both	404:407	Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further.
36974935	7	38	theme	Spontaneous	1011:1021	arg1	Raman					1023:1027	Spontaneous Raman	1011:1027	Spontaneous Raman	1011:1027	Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.
36974935	3	39	theme	useful	392:397	arg1	tool					399:402	a useful tool	390:402	a useful tool both for pharmaceutical development as well as probing the proteome and glycome further	390:490	Identifying the glycans on a protein is a useful tool both for pharmaceutical development as well as probing the proteome and glycome further.
36974935	2	40	theme	translational	322:334	arg1	modification					336:347	the most common post translational modification	301:347	the most common post translational modification	301:347	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	2	40	theme	translational	322:334	arg1	Glycosylation					235:247	Glycosylation	235:247	Glycosylation	235:247	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	6	41	from	changes	925:931	arg1	spectra					949:955	the observed spectra	936:955	the observed spectra associated with the structure of the monosaccharides	936:1008	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	2	42	theme	sugar	270:274	arg1	residue					276:282	a sugar residue	268:282	a sugar residue	268:282	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	6	43	theme	monosaccharides	994:1008	arg1	structure					977:985	the structure	973:985	the structure of the monosaccharides	973:1008	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	1	44	theme	numerous	135:142	arg1	processes					155:163	numerous biological processes	135:163	numerous biological processes	135:163	Sugars play important roles in numerous biological processes, from providing energy to modifying proteins to alter their function.
36974935	1	45	theme	biological	144:153	arg1	processes					155:163	numerous biological processes	135:163	numerous biological processes	135:163	Sugars play important roles in numerous biological processes, from providing energy to modifying proteins to alter their function.
36974935	2	46	theme	post	317:320	arg1	modification					336:347	the most common post translational modification	301:347	the most common post translational modification	301:347	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	2	46	theme	post	317:320	arg1	Glycosylation					235:247	Glycosylation	235:247	Glycosylation	235:247	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	0	47	theme	enhanced	75:82	arg1	spectroscopy					90:101	Raman and surface enhanced Raman spectroscopy	57:101	Raman and surface enhanced Raman spectroscopy	57:101	Chemical conjugation to differentiate monosaccharides by Raman and surface enhanced Raman spectroscopy.
36974935	6	48	theme	sugars	792:797	arg1	scattering					774:783	The weak scattering	765:783	The weak scattering of the sugars	765:797	The weak scattering of the sugars was overcome through conjugation with phenylboronic acid to provide a larger Raman scattering cross section and induce slight changes in the observed spectra associated with the structure of the monosaccharides.
36974935	2	49	attach	attachment	254:263	arg2	residue					276:282	a sugar residue	268:282	a sugar residue	268:282	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	2	49	attach	attachment	254:263	arg1	protein					289:295	a protein	287:295	a protein	287:295	Glycosylation, the attachment of a sugar residue to a protein, is the most common post translational modification.
36974935	7	50	from	SERS	1030:1033	arg1	flow					1038:1041	flow	1038:1041	flow	1038:1041	Spontaneous Raman, SERS in flow, and static SERS detection were performed in order to discriminate between arabinose, fructose, galactose, glucose, mannose, and ribose, as well as provide a method for identification and quantification for these sugar conjugates.
36256567	13	0	theme	name	1743:1746	arg1	sp					1770:1771	the name Luteolibacter marinus sp	1739:1771	the name Luteolibacter marinus sp	1739:1771	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	11	1	theme	species	1494:1500	arg1	delineation					1502:1512	prokaryotic species delineation	1482:1512	prokaryotic species delineation	1482:1512	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	1	2	from	bacterium	40:48	arg1	Verrucomicrobiaceae					64:82	the family Verrucomicrobiaceae	53:82	the family Verrucomicrobiaceae	53:82	nov., a novel bacterium in the family Verrucomicrobiaceae, isolated from marine sediment.
36256567	9	3	dep	%	937:937	arg1	93.8					933:936	93.8	933:936	93.8	933:936	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	9	3	dep	%	937:937	arg1	94.8					924:927	94.8	924:927	94.8	924:927	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	9	4	theme	rRNA	867:870	arg1	sequence					877:884	The 16S rRNA gene sequence	859:884	The 16S rRNA gene sequence of strain NBU1238T	859:903	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	2	5	theme	located	269:275	arg1	Island					262:267	Meishan Island	254:267	Meishan Island located in the East China Sea	254:297	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	11	6	theme	digital	1338:1344	arg1	hybridization					1354:1366	digital DNA-DNA hybridization	1338:1366	digital DNA-DNA hybridization	1338:1366	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	5	7	theme	Tweens	561:566	arg1	hydrolysis					528:537	hydrolysis	528:537	hydrolysis	528:537	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	5	7	theme	Tweens	561:566	arg1	reaction					493:500	Methyl red reaction	482:500	Methyl red reaction	482:500	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	5	7	theme	Tweens	561:566	arg1	test					519:522	Voges-Proskauer test	503:522	Voges-Proskauer test	503:522	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	2	8	theme	Meishan	254:260	arg1	Island					262:267	Meishan Island	254:267	Meishan Island located in the East China Sea	254:297	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	11	9	theme	average	1306:1312	arg1	identity					1325:1332	average nucleotide identity	1306:1332	average nucleotide identity	1306:1332	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	13	10	theme	novel	1687:1691	arg1	species					1693:1699	a novel species	1685:1699	a novel species	1685:1699	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	2	11	theme	China	289:293	arg1	Sea					295:297	the East China Sea	280:297	the East China Sea	280:297	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	12	12	theme	DNA	1550:1552	arg1	content					1527:1533	The DNA G+C content	1515:1533	The DNA G+C content of the genomic DNA	1515:1552	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	12	12	theme	DNA	1550:1552	arg1	%					1566:1566	65.0 mol%	1558:1566	65.0 mol%	1558:1566	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	9	13	dep	similarity	948:957	arg1	%					937:937	%	937:937	%	937:937	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	13	14	from	Luteolibacter	1714:1726	arg1	representative					1667:1680	a representative	1665:1680	a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp	1665:1771	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	13	14	from	Luteolibacter	1714:1726	arg1	NBU1238T					1636:1643	strain NBU1238T	1629:1643	strain NBU1238T	1629:1643	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	7	15	theme	only	680:683	arg1	quinone					697:703	The only respiratory quinone	676:703	The only respiratory quinone	676:703	The only respiratory quinone was menaquinone-9.
36256567	7	15	theme	only	680:683	arg1	menaquinone-9					709:721	menaquinone-9	709:721	menaquinone-9	709:721	The only respiratory quinone was menaquinone-9.
36256567	6	16	theme	fatty	612:616	arg1	iso-C14 					629:636	iso-C14 	629:636	iso-C14 	629:636	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	16	theme	fatty	612:616	arg1	acids					618:622	The major cellular fatty acids	593:622	The major cellular fatty acids	593:622	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	12	17	theme	65.0 mol	1558:1565	arg1	content					1527:1533	The DNA G+C content	1515:1533	The DNA G+C content of the genomic DNA	1515:1552	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	12	17	theme	65.0 mol	1558:1565	arg1	%					1566:1566	65.0 mol%	1558:1566	65.0 mol%	1558:1566	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	5	18	theme	Methyl	482:487	arg1	reaction					493:500	Methyl red reaction	482:500	Methyl red reaction	482:500	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	11	19	theme	DNA-DNA	1346:1352	arg1	hybridization					1354:1366	digital DNA-DNA hybridization	1338:1366	digital DNA-DNA hybridization	1338:1366	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	15	20	theme	type	1796:1799	arg1	strain					1801:1806	The type strain	1792:1806	The type strain	1792:1806	The type strain is NBU1238T (=KCTC 82227T=MCCC 1K04772T).
36256567	15	20	theme	type	1796:1799	arg1	NBU1238T					1811:1818	NBU1238T	1811:1818	NBU1238T (=KCTC 82227T=MCCC 1K04772T)	1811:1847	The type strain is NBU1238T (=KCTC 82227T=MCCC 1K04772T).
36256567	13	21	theme	marinus	1762:1768	arg1	sp					1770:1771	the name Luteolibacter marinus sp	1739:1771	the name Luteolibacter marinus sp	1739:1771	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	11	22	theme	Luteolibacter	1428:1440	arg1	NBU1238T					1390:1397	strain NBU1238T	1383:1397	strain NBU1238T	1383:1397	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	11	22	theme	Luteolibacter	1428:1440	arg1	species					1411:1417	related species	1403:1417	related species of genus Luteolibacter	1403:1440	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	13	23	from	representative	1667:1680	arg1	Luteolibacter					1714:1726	the genus Luteolibacter	1704:1726	the genus Luteolibacter	1704:1726	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	2	24	theme	marine	227:232	arg1	sediment					234:241	marine sediment	227:241	marine sediment sampled on Meishan Island located in the East China Sea	227:297	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	12	25	theme	DNA	1519:1521	arg1	content					1527:1533	The DNA G+C content	1515:1533	The DNA G+C content of the genomic DNA	1515:1552	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	12	25	theme	DNA	1519:1521	arg1	%					1566:1566	65.0 mol%	1558:1566	65.0 mol%	1558:1566	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	8	26	theme	major	728:732	arg1	diphosphatidylglycerol					752:773	diphosphatidylglycerol	752:773	diphosphatidylglycerol	752:773	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine.
36256567	8	26	theme	major	728:732	arg1	lipids					740:745	The major polar lipids	724:745	The major polar lipids	724:745	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine.
36256567	13	27	from	species	1693:1699	arg1	Luteolibacter					1714:1726	the genus Luteolibacter	1704:1726	the genus Luteolibacter	1704:1726	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	11	28	theme	hybridization	1354:1366	arg1	values					1368:1373	The average nucleotide identity and digital DNA-DNA hybridization values	1302:1373	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter	1302:1440	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	4	29	theme	H2S	417:419	arg1	production					421:430	H2S production	417:430	H2S production	417:430	Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive.
36256567	13	30	theme	genotypic	1613:1621	arg1	data					1623:1626	its phenotypic, chemotaxonomic and genotypic data	1578:1626	its phenotypic, chemotaxonomic and genotypic data	1578:1626	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	5	31	theme	Voges-Proskauer	503:517	arg1	test					519:522	Voges-Proskauer test	503:522	Voges-Proskauer test	503:522	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	1	32	theme	family	57:62	arg1	Verrucomicrobiaceae					64:82	the family Verrucomicrobiaceae	53:82	the family Verrucomicrobiaceae	53:82	nov., a novel bacterium in the family Verrucomicrobiaceae, isolated from marine sediment.
36256567	3	33	theme	no	375:376	arg1	NaCl					378:381	no NaCl	375:381	no NaCl	375:381	Strain NBU1238T was able to grow optimally at 28-32 °C, at pH 7.5 and with no NaCl.
36256567	0	34	theme	Luteolibacter	0:12	arg1	sp					22:23	Luteolibacter marinus sp	0:23	Luteolibacter marinus sp.	0:24	Luteolibacter marinus sp.
36256567	13	35	theme	phenotypic	1582:1591	arg1	data					1623:1626	its phenotypic, chemotaxonomic and genotypic data	1578:1626	its phenotypic, chemotaxonomic and genotypic data	1578:1626	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	6	36	dep	iso-C14 	629:636	arg1	C16 					642:645	C16 	642:645	C16 	642:645	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	36	dep	iso-C14 	629:636	arg1	 0					638:639	 0	638:639	 0	638:639	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	37	theme	cellular	603:610	arg1	iso-C14 					629:636	iso-C14 	629:636	iso-C14 	629:636	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	37	theme	cellular	603:610	arg1	acids					618:622	The major cellular fatty acids	593:622	The major cellular fatty acids	593:622	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	13	38	theme	chemotaxonomic	1594:1607	arg1	data					1623:1626	its phenotypic, chemotaxonomic and genotypic data	1578:1626	its phenotypic, chemotaxonomic and genotypic data	1578:1626	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	11	39	theme	related	1403:1409	arg1	species					1411:1417	related species	1403:1417	related species of genus Luteolibacter	1403:1440	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	2	40	theme	non-motile	145:154	arg1	bacterium					177:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	9	41	theme	MC	1047:1048	arg1	3726T					1050:1054	MC 3726T	1047:1054	Luteolibacter arcticus MC 3726T	1024:1054	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	15	42	theme	82227T=MCCC	1827:1837	arg1	1K04772T					1839:1846	=KCTC 82227T=MCCC 1K04772T	1821:1846	=KCTC 82227T=MCCC 1K04772T	1821:1846	The type strain is NBU1238T (=KCTC 82227T=MCCC 1K04772T).
36256567	15	42	theme	82227T=MCCC	1827:1837	arg1	NBU1238T					1811:1818	NBU1238T	1811:1818	NBU1238T (=KCTC 82227T=MCCC 1K04772T)	1811:1847	The type strain is NBU1238T (=KCTC 82227T=MCCC 1K04772T).
36256567	10	43	theme	arcticus	1260:1267	arg1	3726T					1272:1276	L. arcticus MC 3726T	1257:1276	L. arcticus MC 3726T	1257:1276	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	11	44	theme	nucleotide	1314:1323	arg1	identity					1325:1332	average nucleotide identity	1306:1332	average nucleotide identity	1306:1332	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	9	45	theme	sequence	939:946	arg1	similarity					948:957	95.6, 95.6, 94.8 and 93.8% sequence similarity	912:957	95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T	912:1093	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	1	46	theme	novel	34:38	arg1	bacterium					40:48	a novel bacterium	32:48	a novel bacterium in the family Verrucomicrobiaceae	32:82	nov., a novel bacterium in the family Verrucomicrobiaceae, isolated from marine sediment.
36256567	1	46	theme	novel	34:38	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel bacterium in the family Verrucomicrobiaceae, isolated from marine sediment.
36256567	9	47	theme	16S	863:865	arg1	rRNA					867:870	The 16S rRNA	859:870	The 16S rRNA gene sequence of strain NBU1238T	859:903	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	9	48	theme	gene	872:875	arg1	sequence					877:884	The 16S rRNA gene sequence	859:884	The 16S rRNA gene sequence of strain NBU1238T	859:903	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	2	49	theme	rod-shaped	133:142	arg1	bacterium					177:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	11	50	theme	prokaryotic	1482:1492	arg1	delineation					1502:1512	prokaryotic species delineation	1482:1512	prokaryotic species delineation	1482:1512	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	9	51	theme	strain	889:894	arg1	NBU1238T					896:903	strain NBU1238T	889:903	strain NBU1238T	889:903	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	15	52	theme	=KCTC	1821:1825	arg1	1K04772T					1839:1846	=KCTC 82227T=MCCC 1K04772T	1821:1846	=KCTC 82227T=MCCC 1K04772T	1821:1846	The type strain is NBU1238T (=KCTC 82227T=MCCC 1K04772T).
36256567	15	52	theme	=KCTC	1821:1825	arg1	NBU1238T					1811:1818	NBU1238T	1811:1818	NBU1238T (=KCTC 82227T=MCCC 1K04772T)	1811:1847	The type strain is NBU1238T (=KCTC 82227T=MCCC 1K04772T).
36256567	2	53	theme	Gram-negative	118:130	arg1	bacterium					177:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	9	54	theme	NBU1238T	896:903	arg1	sequence					877:884	The 16S rRNA gene sequence	859:884	The 16S rRNA gene sequence of strain NBU1238T	859:903	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	2	55	theme	East	284:287	arg1	Sea					295:297	the East China Sea	280:297	the East China Sea	280:297	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	4	56	theme	oxidase	397:403	arg1	activities					405:414	Catalase and oxidase activities	384:414	Catalase and oxidase activities	384:414	Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive.
36256567	2	57	from	Sea	295:297	arg1	located					269:275	located	269:275	located	269:275	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	7	58	theme	respiratory	685:695	arg1	quinone					697:703	The only respiratory quinone	676:703	The only respiratory quinone	676:703	The only respiratory quinone was menaquinone-9.
36256567	7	58	theme	respiratory	685:695	arg1	menaquinone-9					709:721	menaquinone-9	709:721	menaquinone-9	709:721	The only respiratory quinone was menaquinone-9.
36256567	13	59	theme	species	1693:1699	arg1	representative					1667:1680	a representative	1665:1680	a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp	1665:1771	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	13	59	theme	species	1693:1699	arg1	NBU1238T					1636:1643	strain NBU1238T	1629:1643	strain NBU1238T	1629:1643	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	5	60	theme	red	489:491	arg1	reaction					493:500	Methyl red reaction	482:500	Methyl red reaction	482:500	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	1	61	theme	marine	99:104	arg1	sediment					106:113	marine sediment	99:113	marine sediment	99:113	nov., a novel bacterium in the family Verrucomicrobiaceae, isolated from marine sediment.
36256567	12	62	theme	genomic	1542:1548	arg1	DNA					1550:1552	the genomic DNA	1538:1552	the genomic DNA	1538:1552	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	10	63	theme	genus	1182:1186	arg1	Luteolibacter					1188:1200	the genus Luteolibacter	1178:1200	the genus Luteolibacter	1178:1200	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	4	64	theme	Catalase	384:391	arg1	activities					405:414	Catalase and oxidase activities	384:414	Catalase and oxidase activities	384:414	Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive.
36256567	11	65	theme	genus	1422:1426	arg1	Luteolibacter					1428:1440	genus Luteolibacter	1422:1440	genus Luteolibacter	1422:1440	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	10	66	theme	L.	1257:1258	arg1	3726T					1272:1276	L. arcticus MC 3726T	1257:1276	L. arcticus MC 3726T	1257:1276	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	2	67	from	located	269:275	arg1	Sea					295:297	the East China Sea	280:297	the East China Sea	280:297	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	12	68	theme	G+C	1523:1525	arg1	content					1527:1533	The DNA G+C content	1515:1533	The DNA G+C content of the genomic DNA	1515:1552	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	12	68	theme	G+C	1523:1525	arg1	%					1566:1566	65.0 mol%	1558:1566	65.0 mol%	1558:1566	The DNA G+C content of the genomic DNA was 65.0 mol%.
36256567	4	69	theme	Tweens	450:455	arg1	hydrolysis					436:445	hydrolysis	436:445	hydrolysis of Tweens 40 and 60	436:465	Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive.
36256567	4	69	theme	Tweens	450:455	arg1	activities					405:414	Catalase and oxidase activities	384:414	Catalase and oxidase activities	384:414	Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive.
36256567	4	69	theme	Tweens	450:455	arg1	production					421:430	H2S production	417:430	H2S production	417:430	Catalase and oxidase activities, H2S production and hydrolysis of Tweens 40 and 60 were positive.
36256567	11	70	theme	threshold	1462:1470	arg1	limit					1472:1476	the threshold limit	1458:1476	the threshold limit for prokaryotic species delineation	1458:1512	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	10	71	dep	strains	1229:1235	arg1	3726T					1272:1276	L. arcticus MC 3726T	1257:1276	L. arcticus MC 3726T	1257:1276	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	10	71	dep	strains	1229:1235	arg1	GKXT					1251:1254	L. flavescens GKXT	1237:1254	L. flavescens GKXT	1237:1254	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	10	71	dep	strains	1229:1235	arg1	luteus					1285:1290	L. luteus	1282:1290	L. luteus	1282:1290	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	2	72	attach	isolated	213:220	arg1	sediment					234:241	marine sediment	227:241	marine sediment sampled on Meishan Island located in the East China Sea	227:297	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	2	72	attach	isolated	213:220	arg2	bacterium					177:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	3	73	theme	Strain	300:305	arg1	NBU1238T					307:314	Strain NBU1238T	300:314	Strain NBU1238T	300:314	Strain NBU1238T was able to grow optimally at 28-32 °C, at pH 7.5 and with no NaCl.
36256567	5	74	theme	starch	542:547	arg1	hydrolysis					528:537	hydrolysis	528:537	hydrolysis	528:537	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	5	74	theme	starch	542:547	arg1	reaction					493:500	Methyl red reaction	482:500	Methyl red reaction	482:500	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	5	74	theme	starch	542:547	arg1	test					519:522	Voges-Proskauer test	503:522	Voges-Proskauer test	503:522	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	11	75	theme	strain	1383:1388	arg1	NBU1238T					1390:1397	strain NBU1238T	1383:1397	strain NBU1238T	1383:1397	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	5	76	theme	casein	550:555	arg1	hydrolysis					528:537	hydrolysis	528:537	hydrolysis	528:537	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	5	76	theme	casein	550:555	arg1	reaction					493:500	Methyl red reaction	482:500	Methyl red reaction	482:500	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	5	76	theme	casein	550:555	arg1	test					519:522	Voges-Proskauer test	503:522	Voges-Proskauer test	503:522	Methyl red reaction, Voges-Proskauer test and hydrolysis of starch, casein and Tweens 20 and 80 were negative.
36256567	8	77	theme	polar	734:738	arg1	diphosphatidylglycerol					752:773	diphosphatidylglycerol	752:773	diphosphatidylglycerol	752:773	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine.
36256567	8	77	theme	polar	734:738	arg1	lipids					740:745	The major polar lipids	724:745	The major polar lipids	724:745	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylmethylethanolamine.
36256567	0	78	theme	marinus	14:20	arg1	sp					22:23	Luteolibacter marinus sp	0:23	Luteolibacter marinus sp.	0:24	Luteolibacter marinus sp.
36256567	13	79	theme	strain	1629:1634	arg1	representative					1667:1680	a representative	1665:1680	a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp	1665:1771	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	13	79	theme	strain	1629:1634	arg1	NBU1238T					1636:1643	strain NBU1238T	1629:1643	strain NBU1238T	1629:1643	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	13	80	theme	Luteolibacter	1748:1760	arg1	sp					1770:1771	the name Luteolibacter marinus sp	1739:1771	the name Luteolibacter marinus sp	1739:1771	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
36256567	2	81	theme	aerobic	169:175	arg1	bacterium					177:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium	116:185	A Gram-negative, rod-shaped, non-motile and strictly aerobic bacterium, designated NBU1238T, was isolated from marine sediment sampled on Meishan Island located in the East China Sea.
36256567	6	82	theme	major	597:601	arg1	iso-C14 					629:636	iso-C14 	629:636	iso-C14 	629:636	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	82	theme	major	597:601	arg1	acids					618:622	The major cellular fatty acids	593:622	The major cellular fatty acids	593:622	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	9	83	dep	Luteolibacter	993:1005	arg1	G-1-1-1T					1014:1021	G-1-1-1T	1014:1021	G-1-1-1T	1014:1021	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	9	83	dep	Luteolibacter	993:1005	arg1	luteus					1007:1012	Luteolibacter luteus G-1-1-1T	993:1021	Luteolibacter luteus G-1-1-1T	993:1021	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	10	84	theme	MC	1269:1270	arg1	3726T					1272:1276	L. arcticus MC 3726T	1257:1276	L. arcticus MC 3726T	1257:1276	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	6	85	dep	 0	638:639	arg1	 0					672:673	 0	672:673	 0	672:673	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	85	dep	 0	638:639	arg1	C14 					667:670	C14 	667:670	C14 	667:670	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	85	dep	 0	638:639	arg1	C16 					651:654	C16 	651:654	C16 	651:654	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	6	85	dep	 0	638:639	arg1	ω9c					659:661	ω9c	659:661	ω9c	659:661	The major cellular fatty acids were iso-C14 : 0, C16 : 0, C16 : 1 ω9c and C14 : 0.
36256567	10	86	dep	GKXT	1251:1254	arg1	G-1-1-1T					1292:1299	G-1-1-1T	1292:1299	G-1-1-1T	1292:1299	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	10	87	theme	Phylogenetic	1110:1121	arg1	analyses					1123:1130	Phylogenetic analyses	1110:1130	Phylogenetic analyses	1110:1130	Phylogenetic analyses indicated that strain NBU1238T clustered with the genus Luteolibacter and was closely related to strains L. flavescens GKXT, L. arcticus MC 3726T and L. luteus G-1-1-1T.
36256567	9	88	dep	Luteolibacter	1024:1036	arg1	3726T					1050:1054	MC 3726T	1047:1054	Luteolibacter arcticus MC 3726T	1024:1054	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	9	88	dep	Luteolibacter	1024:1036	arg1	arcticus					1038:1045	Luteolibacter arcticus MC 3726T	1024:1054	Luteolibacter arcticus MC 3726T	1024:1054	The 16S rRNA gene sequence of strain NBU1238T showed 95.6, 95.6, 94.8 and 93.8% sequence similarity to Luteolibacter flavescens GKXT, Luteolibacter luteus G-1-1-1T, Luteolibacter arcticus MC 3726T and Luteolibacter pohnpeiensis A4T-83T, respectively.
36256567	11	89	theme	identity	1325:1332	arg1	values					1368:1373	The average nucleotide identity and digital DNA-DNA hybridization values	1302:1373	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter	1302:1440	The average nucleotide identity and digital DNA-DNA hybridization values between strain NBU1238T and related species of genus Luteolibacter were well below the threshold limit for prokaryotic species delineation.
36256567	13	90	theme	genus	1708:1712	arg1	Luteolibacter					1714:1726	the genus Luteolibacter	1704:1726	the genus Luteolibacter	1704:1726	Based on its phenotypic, chemotaxonomic and genotypic data, strain NBU1238T is considered to be a representative of a novel species in the genus Luteolibacter, for which the name Luteolibacter marinus sp.
35638642	0	0	theme	autophagy	91:99	arg1	activation					101:110	autophagy activation	91:110	autophagy activation in mice	91:118	Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice.
35638642	1	1	theme	lysosomal	311:319	arg1	stress					321:326	lysosomal stress	311:326	lysosomal stress	311:326	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	7	2	theme	IBD	1228:1230	arg1	recovery					1232:1239	IBD recovery	1228:1239	IBD recovery	1228:1239	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	8	3	theme	IBD	1539:1541	arg1	progression					1524:1534	progression	1524:1534	progression	1524:1534	Dietary composition and lifestyle are crucial for pathogenesis and progression of IBD.
35638642	8	3	theme	IBD	1539:1541	arg1	pathogenesis					1507:1518	pathogenesis	1507:1518	pathogenesis	1507:1518	Dietary composition and lifestyle are crucial for pathogenesis and progression of IBD.
35638642	3	4	theme	chlorophyllin	664:676	arg1	administration					646:659	oral administration	641:659	oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll	641:723	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	7	5	from	beneficial	1213:1222	arg1	part					1244:1247	part	1244:1247	part	1244:1247	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	1	6	attach	derived	339:345	arg2	complications					231:243	intestinal complications	220:243	intestinal complications	220:243	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	6	attach	derived	339:345	arg2	diseases					140:147	Inflammatory bowel diseases	121:147	Inflammatory bowel diseases (IBD)	121:153	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	6	attach	derived	339:345	arg1	factors					398:404	environmental risk factors	379:404	environmental risk factors	379:404	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	6	attach	derived	339:345	arg2	disease					174:180	Crohn's disease	166:180	Crohn's disease (CD)	166:185	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	6	attach	derived	339:345	arg2	colitis					202:208	ulcerative colitis	191:208	ulcerative colitis (UC)	191:213	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	6	attach	derived	339:345	arg1	predisposition					360:373	genetic predisposition	352:373	genetic predisposition	352:373	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	7	theme	intestinal	220:229	arg1	diseases					140:147	Inflammatory bowel diseases	121:147	Inflammatory bowel diseases (IBD)	121:153	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	7	theme	intestinal	220:229	arg1	disease					174:180	Crohn's disease	166:180	Crohn's disease (CD)	166:185	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	7	theme	intestinal	220:229	arg1	colitis					202:208	ulcerative colitis	191:208	ulcerative colitis (UC)	191:213	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	7	theme	intestinal	220:229	arg1	complications					231:243	intestinal complications	220:243	intestinal complications	220:243	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	7	8	theme	vegetables	1177:1186	arg1	consumption					1156:1166	consumption	1156:1166	consumption of green vegetables and chlorophyllin	1156:1204	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	3	9	theme	water-soluble	687:699	arg1	chlorophyllin					664:676	chlorophyllin	664:676	chlorophyllin (CHL)	664:682	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	3	9	theme	water-soluble	687:699	arg1	derivate					701:708	a water-soluble derivate	685:708	a water-soluble derivate of chlorophyll	685:723	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	5	10	theme	intestinal	959:968	arg1	tissue					970:975	intestinal tissue	959:975	intestinal tissue	959:975	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	4	11	theme	DSS-treated	729:739	arg1	mice					741:744	DSS-treated mice	729:744	DSS-treated mice	729:744	In DSS-treated mice, autophagy was persistently activated in intestinal tissues and associated with bowel disorders.
35638642	10	12	theme	water-soluble	1674:1686	arg1	Chlorophyllin					1651:1663	Chlorophyllin	1651:1663	Chlorophyllin (CHL)	1651:1669	Chlorophyllin (CHL), a water-soluble derivate of chlorophyll, can attenuate colitis by regulating autophagy and inflammation.
35638642	10	12	theme	water-soluble	1674:1686	arg1	derivate					1688:1695	a water-soluble derivate	1672:1695	a water-soluble derivate of chlorophyll	1672:1710	Chlorophyllin (CHL), a water-soluble derivate of chlorophyll, can attenuate colitis by regulating autophagy and inflammation.
35638642	0	13	theme	bowel	61:65	arg1	diseases					67:74	inflammatory bowel diseases	48:74	inflammatory bowel diseases	48:74	Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice.
35638642	6	14	theme	autophagic	1119:1128	arg1	flux					1144:1147	autophagic and lysosomal flux	1119:1147	autophagic and lysosomal flux	1119:1147	In vitro studies show that CHL could activate Akt and mTOR pathways, leading to downregulation of autophagic and lysosomal flux.
35638642	4	15	theme	bowel	826:830	arg1	disorders					832:840	bowel disorders	826:840	bowel disorders	826:840	In DSS-treated mice, autophagy was persistently activated in intestinal tissues and associated with bowel disorders.
35638642	7	16	from	part	1244:1247	arg1	beneficial					1213:1222	beneficial	1213:1222	beneficial	1213:1222	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	17	theme	bowel	1338:1342	arg1	IBD					1353:1355	IBD	1353:1355	IBD	1353:1355	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	17	theme	bowel	1338:1342	arg1	disease					1344:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease	1289:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1289:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	0	18	theme	inflammatory	48:59	arg1	diseases					67:74	inflammatory bowel diseases	48:74	inflammatory bowel diseases	48:74	Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice.
35638642	7	19	theme	disease	1344:1350	arg1	alleviation					1257:1267	alleviation	1257:1267	alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1257:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	19	theme	disease	1344:1350	arg1	disease					1402:1408	a chronic and recurrent gastrointestinal disease	1361:1408	a chronic and recurrent gastrointestinal disease	1361:1408	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	0	20	theme	green	6:10	arg1	pigment					12:18	Plant green pigment	0:18	Plant green pigment of chlorophyllin	0:35	Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice.
35638642	6	21	dep	In	1021:1022	arg1	vitro					1024:1028	vitro	1024:1028	vitro	1024:1028	In vitro studies show that CHL could activate Akt and mTOR pathways, leading to downregulation of autophagic and lysosomal flux.
35638642	3	22	from	epithelia	604:612	arg1	mice					617:620	mice	617:620	mice	617:620	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	7	23	theme	NOTEWORTHY	1314:1323	arg1	IBD					1353:1355	IBD	1353:1355	IBD	1353:1355	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	23	theme	NOTEWORTHY	1314:1323	arg1	disease					1344:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease	1289:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1289:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	0	24	theme	Plant	0:4	arg1	pigment					12:18	Plant green pigment	0:18	Plant green pigment of chlorophyllin	0:35	Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice.
35638642	11	25	theme	green	1798:1802	arg1	vegetables					1804:1813	green vegetables	1798:1813	green vegetables	1798:1813	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery.
35638642	3	26	theme	oral	641:644	arg1	administration					646:659	oral administration	641:659	oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll	641:723	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	6	27	theme	flux	1144:1147	arg1	downregulation					1101:1114	downregulation	1101:1114	downregulation of autophagic and lysosomal flux	1101:1147	In vitro studies show that CHL could activate Akt and mTOR pathways, leading to downregulation of autophagic and lysosomal flux.
35638642	7	28	theme	Inflammatory	1325:1336	arg1	IBD					1353:1355	IBD	1353:1355	IBD	1353:1355	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	28	theme	Inflammatory	1325:1336	arg1	disease					1344:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease	1289:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1289:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	11	29	theme	vegetables	1804:1813	arg1	consumption					1783:1793	consumption	1783:1793	consumption of green vegetables and chlorophyllin	1783:1831	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery.
35638642	8	30	theme	Dietary	1457:1463	arg1	composition					1465:1475	Dietary composition	1457:1475	Dietary composition	1457:1475	Dietary composition and lifestyle are crucial for pathogenesis and progression of IBD.
35638642	5	31	theme	autophagy	941:949	arg1	flux					951:954	autophagy flux	941:954	autophagy flux	941:954	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	7	32	theme	flux.NEW	1303:1310	arg1	IBD					1353:1355	IBD	1353:1355	IBD	1353:1355	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	32	theme	flux.NEW	1303:1310	arg1	disease					1344:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease	1289:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1289:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	1	33	theme	chronic	262:268	arg1	inflammation					270:281	chronic inflammation	262:281	chronic inflammation	262:281	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	34	theme	genetic	352:358	arg1	predisposition					360:373	genetic predisposition	352:373	genetic predisposition	352:373	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	6	35	dep	Akt	1067:1069	arg1	pathways					1080:1087	pathways	1080:1087	pathways	1080:1087	In vitro studies show that CHL could activate Akt and mTOR pathways, leading to downregulation of autophagic and lysosomal flux.
35638642	7	36	theme	&	1312:1312	arg1	IBD					1353:1355	IBD	1353:1355	IBD	1353:1355	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	36	theme	&	1312:1312	arg1	disease					1344:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease	1289:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1289:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	37	theme	autolysosomal	1289:1301	arg1	IBD					1353:1355	IBD	1353:1355	IBD	1353:1355	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	37	theme	autolysosomal	1289:1301	arg1	disease					1344:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease	1289:1350	autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1289:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	3	38	theme	chlorophyll	713:723	arg1	chlorophyllin					664:676	chlorophyllin	664:676	chlorophyllin (CHL)	664:682	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	3	38	theme	chlorophyll	713:723	arg1	derivate					701:708	a water-soluble derivate	685:708	a water-soluble derivate of chlorophyll	685:723	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	11	39	theme	IBD	1855:1857	arg1	recovery					1859:1866	IBD recovery	1855:1866	IBD recovery	1855:1866	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery.
35638642	3	40	theme	-induced	564:571	arg1	colitis					573:579	dextran sulfate sodium (DSS)-induced colitis	536:579	dextran sulfate sodium (DSS)-induced colitis	536:579	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	3	41	from	colitis	573:579	arg1	mice					617:620	mice	617:620	mice	617:620	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	0	42	theme	chlorophyllin	23:35	arg1	pigment					12:18	Plant green pigment	0:18	Plant green pigment of chlorophyllin	0:35	Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice.
35638642	7	43	theme	gastrointestinal	1385:1400	arg1	alleviation					1257:1267	alleviation	1257:1267	alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1257:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	43	theme	gastrointestinal	1385:1400	arg1	disease					1402:1408	a chronic and recurrent gastrointestinal disease	1361:1408	a chronic and recurrent gastrointestinal disease	1361:1408	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	4	44	theme	intestinal	787:796	arg1	tissues					798:804	intestinal tissues	787:804	intestinal tissues	787:804	In DSS-treated mice, autophagy was persistently activated in intestinal tissues and associated with bowel disorders.
35638642	1	45	theme	ulcerative	191:200	arg1	UC					211:212	UC	211:212	UC	211:212	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	45	theme	ulcerative	191:200	arg1	colitis					202:208	ulcerative colitis	191:208	ulcerative colitis (UC)	191:213	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	2	46	theme	green	446:450	arg1	vegetable					452:460	dietary green vegetable	438:460	dietary green vegetable	438:460	It is generally precepted that dietary green vegetable is beneficial for physiological homeostasis.
35638642	1	47	theme	autophagy	284:292	arg1	abnormality					294:304	autophagy abnormality	284:304	autophagy abnormality	284:304	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	9	48	theme	intestinal	1593:1602	arg1	tissue					1604:1609	the intestinal tissue	1589:1609	the intestinal tissue	1589:1609	In this study, we observed that autophagy in the intestinal tissue was persistently activated in IBD mice.
35638642	1	49	theme	environmental	379:391	arg1	factors					398:404	environmental risk factors	379:404	environmental risk factors	379:404	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	10	50	theme	chlorophyll	1700:1710	arg1	Chlorophyllin					1651:1663	Chlorophyllin	1651:1663	Chlorophyllin (CHL)	1651:1669	Chlorophyllin (CHL), a water-soluble derivate of chlorophyll, can attenuate colitis by regulating autophagy and inflammation.
35638642	10	50	theme	chlorophyll	1700:1710	arg1	derivate					1688:1695	a water-soluble derivate	1672:1695	a water-soluble derivate of chlorophyll	1672:1710	Chlorophyllin (CHL), a water-soluble derivate of chlorophyll, can attenuate colitis by regulating autophagy and inflammation.
35638642	1	51	theme	Inflammatory	121:132	arg1	diseases					140:147	Inflammatory bowel diseases	121:147	Inflammatory bowel diseases (IBD)	121:153	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	51	theme	Inflammatory	121:132	arg1	disease					174:180	Crohn's disease	166:180	Crohn's disease (CD)	166:185	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	51	theme	Inflammatory	121:132	arg1	colitis					202:208	ulcerative colitis	191:208	ulcerative colitis (UC)	191:213	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	51	theme	Inflammatory	121:132	arg1	IBD					150:152	IBD	150:152	IBD	150:152	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	51	theme	Inflammatory	121:132	arg1	complications					231:243	intestinal complications	220:243	intestinal complications	220:243	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	2	52	theme	dietary	438:444	arg1	vegetable					452:460	dietary green vegetable	438:460	dietary green vegetable	438:460	It is generally precepted that dietary green vegetable is beneficial for physiological homeostasis.
35638642	1	53	theme	risk	393:396	arg1	factors					398:404	environmental risk factors	379:404	environmental risk factors	379:404	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	7	54	theme	chronic	1363:1369	arg1	alleviation					1257:1267	alleviation	1257:1267	alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1257:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	54	theme	chronic	1363:1369	arg1	disease					1402:1408	a chronic and recurrent gastrointestinal disease	1361:1408	a chronic and recurrent gastrointestinal disease	1361:1408	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	1	55	theme	bowel	134:138	arg1	diseases					140:147	Inflammatory bowel diseases	121:147	Inflammatory bowel diseases (IBD)	121:153	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	55	theme	bowel	134:138	arg1	disease					174:180	Crohn's disease	166:180	Crohn's disease (CD)	166:185	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	55	theme	bowel	134:138	arg1	colitis					202:208	ulcerative colitis	191:208	ulcerative colitis (UC)	191:213	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	55	theme	bowel	134:138	arg1	IBD					150:152	IBD	150:152	IBD	150:152	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	1	55	theme	bowel	134:138	arg1	complications					231:243	intestinal complications	220:243	intestinal complications	220:243	Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are intestinal complications characterized by chronic inflammation, autophagy abnormality, and lysosomal stress, which are derived from genetic predisposition and environmental risk factors.
35638642	9	56	from	autophagy	1576:1584	arg1	tissue					1604:1609	the intestinal tissue	1589:1609	the intestinal tissue	1589:1609	In this study, we observed that autophagy in the intestinal tissue was persistently activated in IBD mice.
35638642	5	57	theme	reticulum	1003:1011	arg1	stress					1013:1018	endoplasmic reticulum stress	991:1018	endoplasmic reticulum stress	991:1018	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	3	58	theme	intestinal	593:602	arg1	epithelia					604:612	altered intestinal epithelia	585:612	altered intestinal epithelia	585:612	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	7	59	theme	recurrent	1375:1383	arg1	alleviation					1257:1267	alleviation	1257:1267	alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1257:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	59	theme	recurrent	1375:1383	arg1	disease					1402:1408	a chronic and recurrent gastrointestinal disease	1361:1408	a chronic and recurrent gastrointestinal disease	1361:1408	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	5	60	theme	intestinal	902:911	arg1	inflammation					913:924	intestinal inflammation	902:924	intestinal inflammation	902:924	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	6	61	theme	lysosomal	1134:1142	arg1	flux					1144:1147	autophagic and lysosomal flux	1119:1147	autophagic and lysosomal flux	1119:1147	In vitro studies show that CHL could activate Akt and mTOR pathways, leading to downregulation of autophagic and lysosomal flux.
35638642	7	62	theme	inflammation	1272:1283	arg1	alleviation					1257:1267	alleviation	1257:1267	alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD)	1257:1356	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	7	62	theme	inflammation	1272:1283	arg1	disease					1402:1408	a chronic and recurrent gastrointestinal disease	1361:1408	a chronic and recurrent gastrointestinal disease	1361:1408	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	5	63	from	supplement	855:864	arg1	diet					876:879	diet	876:879	diet	876:879	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	5	63	from	supplement	855:864	arg1	gavage					884:889	gavage	884:889	gavage	884:889	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	9	64	theme	IBD	1641:1643	arg1	mice					1645:1648	IBD mice	1641:1648	IBD mice	1641:1648	In this study, we observed that autophagy in the intestinal tissue was persistently activated in IBD mice.
35638642	6	65	theme	In	1021:1022	arg1	studies					1030:1036	In vitro studies	1021:1036	In vitro studies	1021:1036	In vitro studies show that CHL could activate Akt and mTOR pathways, leading to downregulation of autophagic and lysosomal flux.
35638642	0	66	from	activation	101:110	arg1	mice					115:118	mice	115:118	mice	115:118	Plant green pigment of chlorophyllin attenuates inflammatory bowel diseases by suppressing autophagy activation in mice.
35638642	5	67	theme	endoplasmic	991:1001	arg1	reticulum					1003:1011	endoplasmic reticulum	991:1011	endoplasmic reticulum stress	991:1018	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	5	68	theme	CHL	869:871	arg1	supplement					855:864	supplement	855:864	supplement of CHL in diet or gavage	855:889	Conversely, supplement of CHL in diet or gavage suppressed intestinal inflammation, downregulated autophagy flux in intestinal tissue, and relieved endoplasmic reticulum stress.
35638642	11	69	theme	chlorophyllin	1819:1831	arg1	consumption					1783:1793	consumption	1783:1793	consumption of green vegetables and chlorophyllin	1783:1831	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery.
35638642	2	70	theme	physiological	480:492	arg1	homeostasis					494:504	physiological homeostasis	480:504	physiological homeostasis	480:504	It is generally precepted that dietary green vegetable is beneficial for physiological homeostasis.
35638642	7	71	theme	green	1171:1175	arg1	vegetables					1177:1186	green vegetables	1171:1186	green vegetables	1171:1186	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
35638642	3	72	theme	altered	585:591	arg1	epithelia					604:612	altered intestinal epithelia	585:612	altered intestinal epithelia	585:612	In this study, we found that dextran sulfate sodium (DSS)-induced colitis and altered intestinal epithelia in mice were attenuated by oral administration of chlorophyllin (CHL), a water-soluble derivate of chlorophyll.
35638642	7	73	theme	chlorophyllin	1192:1204	arg1	consumption					1156:1166	consumption	1156:1166	consumption of green vegetables and chlorophyllin	1156:1204	Thus, consumption of green vegetables and chlorophyllin may be beneficial for IBD recovery in part through alleviation of inflammation and autolysosomal flux.NEW & NOTEWORTHY Inflammatory bowel disease (IBD) is a chronic and recurrent gastrointestinal disease, while the etiology remains poorly understood.
36641976	17	0	theme	structure	2190:2198	arg1	effacement					2159:2168	effacement	2159:2168	effacement	2159:2168	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	17	0	theme	structure	2190:2198	arg1	distortion					2144:2153	severe distortion	2137:2153	severe distortion	2137:2153	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	6	1	theme	periodic	820:827	arg1	AB-PAS					842:847	AB-PAS	842:847	AB-PAS	842:847	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	6	1	theme	periodic	820:827	arg1	acid-Schiff					829:839	periodic acid-Schiff	820:839	periodic acid-Schiff (AB-PAS)	820:848	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	19	2	theme	WB	2437:2438	arg1	validation					2440:2449	WB validation	2437:2449	WB validation	2437:2449	RNA-Seq combined with WB validation showed that XP exerted antidiarrheal effects by inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	8	3	theme	colonic	988:994	arg1	microvilli					996:1005	colonic microvilli	988:1005	colonic microvilli	988:1005	Images of colonic microvilli were obtained by TEM.
36641976	24	4	theme	protective	3568:3577	arg1	mechanism					3579:3587	the protective mechanism	3564:3587	the protective mechanism of HPX	3564:3594	The protective effect of HPX was reversed after transfection with pcDNA 3.1-MAP3K5, which fully demonstrated that the protective mechanism of HPX was achieved by inhibiting MAP3K5 and its downstream pathways.
36641976	21	5	theme	Prevotella	2908:2917	arg1	abundance					2895:2903	the abundance	2891:2903	the abundance of Prevotella 9	2891:2919	Impressively, the correlation analysis between 16S rRNA sequencing and LC-MS suggested that HPX and Prevotella 9 are negatively correlated, which indicated that XP might increase the content of HPX by reducing the abundance of Prevotella 9.
36641976	6	6	theme	blue	810:813	arg1	staining					850:857	Alcian blue plus periodic acid-Schiff (AB-PAS) staining	803:857	Alcian blue plus periodic acid-Schiff (AB-PAS) staining	803:857	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	1	7	theme	diarrhea-predominant	268:287	arg1	syndrome					305:312	diarrhea-predominant irritable bowel syndrome	268:312	diarrhea-predominant irritable bowel syndrome (IBS-D)	268:320	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	7	theme	diarrhea-predominant	268:287	arg1	IBS-D					315:319	IBS-D	315:319	IBS-D	315:319	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	13	8	dep	screened	1428:1435	arg1	CCK8					1461:1464	CCK8	1461:1464	CCK8	1461:1464	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	22	9	theme	negative	2935:2942	arg1	correlation					2944:2954	a negative correlation	2933:2954	a negative correlation between HPX and ASK1	2933:2975	Meanwhile, a negative correlation between HPX and ASK1 was indicated through RNA-Seq and LC-MS, which suggested that the inhibition of ASK1 (Map3k5) may be ascribed to the increase in HPX after XP treatment.
36641976	20	10	theme	untargeted	2647:2656	arg1	analysis					2671:2678	untargeted metabolomics analysis	2647:2678	untargeted metabolomics analysis	2647:2678	Additionally, XP also increased the relative expression level of the metabolite HPX, as revealed by untargeted metabolomics analysis.
36641976	22	11	theme	ASK1	3057:3060	arg1	inhibition					3043:3052	the inhibition	3039:3052	the inhibition of ASK1 (Map3k5)	3039:3069	Meanwhile, a negative correlation between HPX and ASK1 was indicated through RNA-Seq and LC-MS, which suggested that the inhibition of ASK1 (Map3k5) may be ascribed to the increase in HPX after XP treatment.
36641976	18	12	theme	genus	2336:2340	arg1	levels					2342:2347	the phylum and genus levels	2321:2347	levels	2342:2347	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	25	13	theme	CONCLUSION	3659:3668	arg1	XP					3670:3671	CONCLUSION XP	3659:3671	CONCLUSION XP	3659:3671	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	15	14	theme	expression	1867:1876	arg1	changes					1878:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	WB detection of ASK1/P38 MAPK pathway protein expression changes.
36641976	13	15	theme	nontoxic	1387:1394	arg1	range					1396:1400	The nontoxic range	1383:1400	The nontoxic range of hypoxanthine (HPX)	1383:1422	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	1	16	theme	over-the-counter	190:205	arg1	medicine					214:221	a commercially available over-the-counter herbal medicine	165:221	a commercially available over-the-counter herbal medicine in China	165:230	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	16	theme	over-the-counter	190:205	arg1	Capsule					151:157	BACKGROUND Xingpi Capsule	133:157	BACKGROUND Xingpi Capsule (XP)	133:162	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	18	17	theme	phylum	2325:2330	arg1	levels					2342:2347	the phylum and genus levels	2321:2347	levels	2342:2347	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	15	18	theme	pathway	1851:1857	arg1	changes					1878:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	WB detection of ASK1/P38 MAPK pathway protein expression changes.
36641976	10	19	theme	rRNA	1193:1196	arg1	sequencing					1198:1207	rRNA sequencing	1193:1207	rRNA sequencing	1193:1207	The composition of the rat intestinal microbiota was detected by 16S rRNA sequencing.
36641976	14	20	theme	pcDNA3.1-MAP3K5	1662:1676	arg1	transfection					1646:1657	transfection	1646:1657	transfection of pcDNA3.1-MAP3K5	1646:1676	After transfection of pcDNA3.1-MAP3K5, Hoechst 33,342 staining, flow cytometry to detect cell apoptosis, and immunofluorescence to detect the fluorescence changes of ASK1 and ZO-1.
36641976	14	21	theme	ZO-1	1815:1818	arg1	changes					1795:1801	the fluorescence changes	1778:1801	the fluorescence changes of ASK1 and ZO-1	1778:1818	After transfection of pcDNA3.1-MAP3K5, Hoechst 33,342 staining, flow cytometry to detect cell apoptosis, and immunofluorescence to detect the fluorescence changes of ASK1 and ZO-1.
36641976	24	22	with	transfection	3498:3509	arg1	3.1-MAP3K5					3522:3531	pcDNA 3.1-MAP3K5	3516:3531	pcDNA 3.1-MAP3K5	3516:3531	The protective effect of HPX was reversed after transfection with pcDNA 3.1-MAP3K5, which fully demonstrated that the protective mechanism of HPX was achieved by inhibiting MAP3K5 and its downstream pathways.
36641976	12	23	theme	RNA	1357:1359	arg1	RNA-Seq					1373:1379	RNA-Seq	1373:1379	RNA-Seq	1373:1379	Changes in colon RNA expression were assessed by RNA sequencing (RNA-Seq).
36641976	12	23	theme	RNA	1357:1359	arg1	sequencing					1361:1370	RNA sequencing	1357:1370	RNA sequencing (RNA-Seq)	1357:1380	Changes in colon RNA expression were assessed by RNA sequencing (RNA-Seq).
36641976	17	24	theme	microvillous	2177:2188	arg1	structure					2190:2198	the microvillous structure	2173:2198	the microvillous structure	2173:2198	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	15	25	theme	ASK1/P38	1837:1844	arg1	changes					1878:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	WB detection of ASK1/P38 MAPK pathway protein expression changes.
36641976	25	26	theme	microbiota	3839:3848	arg1	metabolite					3821:3830	a metabolite	3819:3830	a metabolite of the microbiota	3819:3848	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	25	26	theme	microbiota	3839:3848	arg1	HPX					3814:3816	HPX	3814:3816	HPX	3814:3816	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	23	27	theme	In	3130:3131	arg1	experiments					3139:3149	In vitro experiments	3130:3149	In vitro experiments	3130:3149	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	24	28	theme	HPX	3475:3477	arg1	effect					3465:3470	The protective effect	3450:3470	The protective effect of HPX	3450:3477	The protective effect of HPX was reversed after transfection with pcDNA 3.1-MAP3K5, which fully demonstrated that the protective mechanism of HPX was achieved by inhibiting MAP3K5 and its downstream pathways.
36641976	25	29	theme	MAPK	3879:3882	arg1	pathway					3894:3900	the ASK1/P38 MAPK signaling pathway	3866:3900	the ASK1/P38 MAPK signaling pathway	3866:3900	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	9	30	theme	Western	1029:1035	arg1	WB					1047:1048	WB	1047:1048	WB	1047:1048	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	9	30	theme	Western	1029:1035	arg1	blotting					1037:1044	Western blotting	1029:1044	Western blotting (WB)	1029:1049	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	23	31	theme	ASK1/P38	3418:3425	arg1	proteins					3440:3447	ASK1/P38 MAPK pathway proteins	3418:3447	ASK1/P38 MAPK pathway proteins	3418:3447	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	23	32	theme	ZO-1	3302:3305	arg1	expression					3307:3316	ZO-1 expression	3302:3316	ZO-1 expression	3302:3316	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	1	33	theme	BACKGROUND	133:142	arg1	XP					160:161	XP	160:161	XP	160:161	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	33	theme	BACKGROUND	133:142	arg1	Capsule					151:157	BACKGROUND Xingpi Capsule	133:157	BACKGROUND Xingpi Capsule (XP)	133:162	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	33	theme	BACKGROUND	133:142	arg1	medicine					214:221	a commercially available over-the-counter herbal medicine	165:221	a commercially available over-the-counter herbal medicine in China	165:230	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	18	34	theme	Desulfurium	2289:2299	arg1	abundance					2276:2284	the abundance	2272:2284	the abundance of Desulfurium and Prevotella 9	2272:2316	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	9	35	used	used	1055:1058	arg2	blotting					1037:1044	Western blotting	1029:1044	Western blotting (WB)	1029:1049	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	9	35	used	used	1055:1058	arg2	WB					1047:1048	WB	1047:1048	WB	1047:1048	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	13	36	theme	human	1486:1490	arg1	NCM460					1518:1523	NCM460	1518:1523	NCM460	1518:1523	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	13	36	theme	human	1486:1490	arg1	cells					1511:1515	human colonic epithelial cells	1486:1515	human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS)	1486:1560	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	14	37	theme	ASK1	1806:1809	arg1	changes					1795:1801	the fluorescence changes	1778:1801	the fluorescence changes of ASK1 and ZO-1	1778:1818	After transfection of pcDNA3.1-MAP3K5, Hoechst 33,342 staining, flow cytometry to detect cell apoptosis, and immunofluorescence to detect the fluorescence changes of ASK1 and ZO-1.
36641976	11	38	from	Changes	1210:1216	arg1	metabolites					1229:1239	colonic metabolites	1221:1239	colonic metabolites	1221:1239	Changes in colonic metabolites were evaluated by liquid chromatography-mass spectrometry (LC-MS).
36641976	0	39	theme	predominant-irritable	54:74	arg1	syndrome					82:89	diarrhea predominant-irritable bowel syndrome	45:89	diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	45:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	19	40	theme	ASK1/P38	2514:2521	arg1	pathway					2538:2544	the ASK1/P38 MAPK signaling pathway	2510:2544	the ASK1/P38 MAPK signaling pathway	2510:2544	RNA-Seq combined with WB validation showed that XP exerted antidiarrheal effects by inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	13	41	theme	epithelial	1500:1509	arg1	NCM460					1518:1523	NCM460	1518:1523	NCM460	1518:1523	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	13	41	theme	epithelial	1500:1509	arg1	cells					1511:1515	human colonic epithelial cells	1486:1515	human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS)	1486:1560	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	24	42	theme	pcDNA	3516:3520	arg1	3.1-MAP3K5					3522:3531	pcDNA 3.1-MAP3K5	3516:3531	pcDNA 3.1-MAP3K5	3516:3531	The protective effect of HPX was reversed after transfection with pcDNA 3.1-MAP3K5, which fully demonstrated that the protective mechanism of HPX was achieved by inhibiting MAP3K5 and its downstream pathways.
36641976	0	43	from	mechanism	14:22	arg1	treatment					91:99	diarrhea predominant-irritable bowel syndrome treatment	45:99	diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	45:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	25	44	theme	intestinal	3747:3756	arg1	microbiota					3758:3767	the intestinal microbiota	3743:3767	the intestinal microbiota	3743:3767	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	0	45	theme	syndrome	82:89	arg1	treatment					91:99	diarrhea predominant-irritable bowel syndrome treatment	45:99	diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	45:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	3	46	from	efficacy	421:428	arg1	IBS-D					433:437	IBS-D	433:437	IBS-D	433:437	PURPOSE This study aimed to investigate XP efficacy in IBS-D and elucidate the underlying molecular mechanisms.
36641976	23	47	theme	MAPK	3427:3430	arg1	proteins					3440:3447	ASK1/P38 MAPK pathway proteins	3418:3447	ASK1/P38 MAPK pathway proteins	3418:3447	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	18	48	theme	genus	2402:2406	arg1	level					2408:2412	the genus level	2398:2412	the genus level	2398:2412	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	16	49	theme	loose	1958:1962	arg1	rate					1970:1973	the loose stool rate	1954:1973	the loose stool rate	1954:1973	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	23	50	theme	cell	3250:3253	arg1	viability					3255:3263	cell viability	3250:3263	cell viability	3250:3263	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	5	51	theme	rat	629:631	arg1	weight					638:643	rat body weight	629:643	rat body weight	629:643	The changes in rat body weight and stool were recorded daily.
36641976	23	52	theme	pathway	3432:3438	arg1	proteins					3440:3447	ASK1/P38 MAPK pathway proteins	3418:3447	ASK1/P38 MAPK pathway proteins	3418:3447	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	23	53	from	intensity	3345:3353	arg1	group					3378:3382	the model group	3368:3382	the model group	3368:3382	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	9	54	theme	protein	1075:1081	arg1	expression					1083:1092	the protein expression	1071:1092	the protein expression of the ASK1/P38 MAPK pathway	1071:1121	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	0	55	theme	multiomics	110:119	arg1	technology					121:130	multiomics technology	110:130	multiomics technology	110:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	19	56	theme	signaling	2528:2536	arg1	pathway					2538:2544	the ASK1/P38 MAPK signaling pathway	2510:2544	the ASK1/P38 MAPK signaling pathway	2510:2544	RNA-Seq combined with WB validation showed that XP exerted antidiarrheal effects by inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	16	57	theme	patients	1933:1940	arg1	weight					1917:1922	the body weight	1908:1922	the body weight of IBS-D patients	1908:1940	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	4	58	theme	rat	500:502	arg1	model					510:514	A rat IBS-D model	498:514	A rat IBS-D model	498:514	METHODS A rat IBS-D model was established by senna decoction gavage combined with restraint stress and swimming exhaustion.
36641976	15	59	theme	changes	1878:1884	arg1	detection					1824:1832	WB detection	1821:1832	WB detection of ASK1/P38 MAPK pathway protein expression changes.	1821:1885	WB detection of ASK1/P38 MAPK pathway protein expression changes.
36641976	20	60	theme	relative	2583:2590	arg1	level					2603:2607	the relative expression level	2579:2607	the relative expression level of the metabolite HPX	2579:2629	Additionally, XP also increased the relative expression level of the metabolite HPX, as revealed by untargeted metabolomics analysis.
36641976	17	61	theme	Occludin	2113:2120	arg1	expression					2099:2108	the expression	2095:2108	the expression of Occludin	2095:2120	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	17	61	theme	Occludin	2113:2120	arg1	number					2068:2073	the number	2064:2073	the number of goblet cells	2064:2089	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	13	62	theme	cell	1472:1475	arg1	model					1477:1481	the cell model	1468:1481	the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS)	1468:1560	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	16	63	theme	stool	1982:1986	arg1	index					1988:1992	loose stool index	1976:1992	loose stool index	1976:1992	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	1	64	theme	irritable	289:297	arg1	syndrome					305:312	diarrhea-predominant irritable bowel syndrome	268:312	diarrhea-predominant irritable bowel syndrome (IBS-D)	268:320	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	64	theme	irritable	289:297	arg1	IBS-D					315:319	IBS-D	315:319	IBS-D	315:319	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	9	65	theme	ASK1/P38	1101:1108	arg1	pathway					1115:1121	the ASK1/P38 MAPK pathway	1097:1121	the ASK1/P38 MAPK pathway	1097:1121	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	18	66	theme	Prevotella	2305:2314	arg1	abundance					2276:2284	the abundance	2272:2284	the abundance of Desulfurium and Prevotella 9	2272:2316	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	6	67	theme	pathological	682:693	arg1	changes					695:701	Colon pathological changes	676:701	Colon pathological changes	676:701	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	18	68	theme	sequence	2229:2236	arg1	analysis					2238:2245	16S rRNA gene sequence analysis	2215:2245	16S rRNA gene sequence analysis	2215:2245	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	25	69	theme	relative	3785:3792	arg1	level					3805:3809	the relative expression level	3781:3809	the relative expression level of HPX, a metabolite of the microbiota	3781:3848	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	13	70	theme	HPX	1614:1616	arg1	concentration					1597:1609	the effective concentration	1583:1609	the effective concentration of HPX	1583:1616	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	9	71	theme	pathway	1115:1121	arg1	expression					1083:1092	the protein expression	1071:1092	the protein expression of the ASK1/P38 MAPK pathway	1071:1121	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	3	72	theme	underlying	457:466	arg1	mechanisms					478:487	the underlying molecular mechanisms	453:487	the underlying molecular mechanisms	453:487	PURPOSE This study aimed to investigate XP efficacy in IBS-D and elucidate the underlying molecular mechanisms.
36641976	0	73	theme	Xingpi	27:32	arg1	Capsule					34:40	Xingpi Capsule	27:40	Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	27:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	10	74	theme	microbiota	1162:1171	arg1	composition					1128:1138	The composition	1124:1138	The composition of the rat intestinal microbiota	1124:1171	The composition of the rat intestinal microbiota was detected by 16S rRNA sequencing.
36641976	18	75	theme	rRNA	2219:2222	arg1	analysis					2238:2245	16S rRNA gene sequence analysis	2215:2245	16S rRNA gene sequence analysis	2215:2245	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	4	76	theme	senna	535:539	arg1	gavage					551:556	senna decoction gavage	535:556	senna decoction gavage combined with restraint stress and swimming exhaustion	535:611	METHODS A rat IBS-D model was established by senna decoction gavage combined with restraint stress and swimming exhaustion.
36641976	11	77	theme	liquid	1259:1264	arg1	LC-MS					1300:1304	LC-MS	1300:1304	LC-MS	1300:1304	Changes in colonic metabolites were evaluated by liquid chromatography-mass spectrometry (LC-MS).
36641976	11	77	theme	liquid	1259:1264	arg1	spectrometry					1286:1297	liquid chromatography-mass spectrometry	1259:1297	liquid chromatography-mass spectrometry (LC-MS)	1259:1305	Changes in colonic metabolites were evaluated by liquid chromatography-mass spectrometry (LC-MS).
36641976	6	78	theme	hematoxylin-eosin	767:783	arg1	staining					790:797	hematoxylin-eosin (HE) staining	767:797	hematoxylin-eosin (HE) staining	767:797	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	14	79	theme	fluorescence	1782:1793	arg1	changes					1795:1801	the fluorescence changes	1778:1801	the fluorescence changes of ASK1 and ZO-1	1778:1818	After transfection of pcDNA3.1-MAP3K5, Hoechst 33,342 staining, flow cytometry to detect cell apoptosis, and immunofluorescence to detect the fluorescence changes of ASK1 and ZO-1.
36641976	16	80	theme	loose	1976:1980	arg1	index					1988:1992	loose stool index	1976:1992	loose stool index	1976:1992	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	14	81	theme	cell	1729:1732	arg1	apoptosis					1734:1742	cell apoptosis	1729:1742	cell apoptosis	1729:1742	After transfection of pcDNA3.1-MAP3K5, Hoechst 33,342 staining, flow cytometry to detect cell apoptosis, and immunofluorescence to detect the fluorescence changes of ASK1 and ZO-1.
36641976	23	82	theme	cell	3275:3278	arg1	apoptosis					3280:3288	cell apoptosis	3275:3288	cell apoptosis	3275:3288	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	16	83	theme	RESULTS	1887:1893	arg1	XP					1895:1896	RESULTS XP	1887:1896	RESULTS XP	1887:1896	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	22	84	from	increase	3094:3101	arg1	HPX					3106:3108	HPX	3106:3108	HPX after XP treatment	3106:3127	Meanwhile, a negative correlation between HPX and ASK1 was indicated through RNA-Seq and LC-MS, which suggested that the inhibition of ASK1 (Map3k5) may be ascribed to the increase in HPX after XP treatment.
36641976	16	85	theme	Bristo	1999:2004	arg1	score					2006:2010	Bristo score	1999:2010	Bristo score	1999:2010	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	13	86	theme	Cell	1440:1443	arg1	Counting					1445:1452	Cell Counting Kit-8	1440:1458	Cell Counting Kit-8	1440:1458	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	17	87	theme	goblet	2078:2083	arg1	cells					2085:2089	goblet cells	2078:2089	goblet cells	2078:2089	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	6	88	theme	rats	745:748	arg1	cells					736:740	colonic goblet cells	721:740	colonic goblet cells of rats	721:748	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	14	89	theme	flow	1704:1707	arg1	cytometry					1709:1717	flow cytometry	1704:1717	flow cytometry to detect cell apoptosis	1704:1742	After transfection of pcDNA3.1-MAP3K5, Hoechst 33,342 staining, flow cytometry to detect cell apoptosis, and immunofluorescence to detect the fluorescence changes of ASK1 and ZO-1.
36641976	20	90	theme	HPX	2627:2629	arg1	level					2603:2607	the relative expression level	2579:2607	the relative expression level of the metabolite HPX	2579:2629	Additionally, XP also increased the relative expression level of the metabolite HPX, as revealed by untargeted metabolomics analysis.
36641976	16	91	theme	body	1912:1915	arg1	weight					1917:1922	the body weight	1908:1922	the body weight of IBS-D patients	1908:1940	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	23	92	theme	NCM460	3198:3203	arg1	damage					3205:3210	LPS-induced NCM460 damage	3186:3210	LPS-induced NCM460 damage	3186:3210	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	6	93	theme	cells	736:740	arg1	changes					695:701	Colon pathological changes	676:701	Colon pathological changes	676:701	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	6	93	theme	cells	736:740	arg1	number					711:716	the number	707:716	the number of colonic goblet cells of rats	707:748	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	7	94	theme	Occludin	892:899	arg1	expression					878:887	The expression	874:887	The expression of Occludin, a tight-junction-associated protein,	874:937	The expression of Occludin, a tight-junction-associated protein, was examined via immunohistochemistry.
36641976	8	95	theme	microvilli	996:1005	arg1	Images					978:983	Images	978:983	Images of colonic microvilli	978:1005	Images of colonic microvilli were obtained by TEM.
36641976	4	96	theme	swimming	593:600	arg1	exhaustion					602:611	swimming exhaustion	593:611	swimming exhaustion	593:611	METHODS A rat IBS-D model was established by senna decoction gavage combined with restraint stress and swimming exhaustion.
36641976	14	97	theme	Hoechst	1679:1685	arg1	staining					1694:1701	Hoechst 33,342 staining	1679:1701	Hoechst 33,342 staining	1679:1701	After transfection of pcDNA3.1-MAP3K5, Hoechst 33,342 staining, flow cytometry to detect cell apoptosis, and immunofluorescence to detect the fluorescence changes of ASK1 and ZO-1.
36641976	12	98	from	Changes	1308:1314	arg1	expression					1329:1338	colon RNA expression	1319:1338	colon RNA expression	1319:1338	Changes in colon RNA expression were assessed by RNA sequencing (RNA-Seq).
36641976	6	99	theme	colonic	721:727	arg1	cells					736:740	colonic goblet cells	721:740	colonic goblet cells of rats	721:748	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	24	100	theme	HPX	3592:3594	arg1	mechanism					3579:3587	the protective mechanism	3564:3587	the protective mechanism of HPX	3564:3594	The protective effect of HPX was reversed after transfection with pcDNA 3.1-MAP3K5, which fully demonstrated that the protective mechanism of HPX was achieved by inhibiting MAP3K5 and its downstream pathways.
36641976	20	101	theme	metabolomics	2658:2669	arg1	analysis					2671:2678	untargeted metabolomics analysis	2647:2678	untargeted metabolomics analysis	2647:2678	Additionally, XP also increased the relative expression level of the metabolite HPX, as revealed by untargeted metabolomics analysis.
36641976	24	102	theme	protective	3454:3463	arg1	effect					3465:3470	The protective effect	3450:3470	The protective effect of HPX	3450:3477	The protective effect of HPX was reversed after transfection with pcDNA 3.1-MAP3K5, which fully demonstrated that the protective mechanism of HPX was achieved by inhibiting MAP3K5 and its downstream pathways.
36641976	20	103	theme	metabolite	2616:2625	arg1	HPX					2627:2629	the metabolite HPX	2612:2629	the metabolite HPX	2612:2629	Additionally, XP also increased the relative expression level of the metabolite HPX, as revealed by untargeted metabolomics analysis.
36641976	10	104	theme	rat	1147:1149	arg1	microbiota					1162:1171	the rat intestinal microbiota	1143:1171	the rat intestinal microbiota	1143:1171	The composition of the rat intestinal microbiota was detected by 16S rRNA sequencing.
36641976	15	105	theme	protein	1859:1865	arg1	changes					1878:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	WB detection of ASK1/P38 MAPK pathway protein expression changes.
36641976	20	106	theme	expression	2592:2601	arg1	level					2603:2607	the relative expression level	2579:2607	the relative expression level of the metabolite HPX	2579:2629	Additionally, XP also increased the relative expression level of the metabolite HPX, as revealed by untargeted metabolomics analysis.
36641976	23	107	theme	model	3372:3376	arg1	group					3378:3382	the model group	3368:3382	the model group	3368:3382	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	18	108	theme	Bacteroides	2383:2393	arg1	abundance					2370:2378	the abundance	2366:2378	the abundance of Bacteroides	2366:2393	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	1	109	theme	available	180:188	arg1	medicine					214:221	a commercially available over-the-counter herbal medicine	165:221	a commercially available over-the-counter herbal medicine in China	165:230	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	109	theme	available	180:188	arg1	Capsule					151:157	BACKGROUND Xingpi Capsule	133:157	BACKGROUND Xingpi Capsule (XP)	133:162	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	15	110	theme	MAPK	1846:1849	arg1	changes					1878:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	ASK1/P38 MAPK pathway protein expression changes	1837:1884	WB detection of ASK1/P38 MAPK pathway protein expression changes.
36641976	6	111	theme	HE	786:787	arg1	staining					790:797	hematoxylin-eosin (HE) staining	767:797	hematoxylin-eosin (HE) staining	767:797	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	1	112	theme	herbal	207:212	arg1	medicine					214:221	a commercially available over-the-counter herbal medicine	165:221	a commercially available over-the-counter herbal medicine in China	165:230	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	112	theme	herbal	207:212	arg1	Capsule					151:157	BACKGROUND Xingpi Capsule	133:157	BACKGROUND Xingpi Capsule (XP)	133:162	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	22	113	theme	XP	3116:3117	arg1	treatment					3119:3127	XP treatment	3116:3127	XP treatment	3116:3127	Meanwhile, a negative correlation between HPX and ASK1 was indicated through RNA-Seq and LC-MS, which suggested that the inhibition of ASK1 (Map3k5) may be ascribed to the increase in HPX after XP treatment.
36641976	0	114	from	treatment	91:99	arg1	mechanism					14:22	the mechanism	10:22	the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	10:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	21	115	theme	16S	2728:2730	arg1	sequencing					2737:2746	16S rRNA sequencing	2728:2746	16S rRNA sequencing	2728:2746	Impressively, the correlation analysis between 16S rRNA sequencing and LC-MS suggested that HPX and Prevotella 9 are negatively correlated, which indicated that XP might increase the content of HPX by reducing the abundance of Prevotella 9.
36641976	25	116	theme	multifaceted	3683:3694	arg1	protection					3696:3705	multifaceted protection	3683:3705	multifaceted protection against IBS-D in rats	3683:3727	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	13	117	theme	hypoxanthine	1405:1416	arg1	range					1396:1400	The nontoxic range	1383:1400	The nontoxic range of hypoxanthine (HPX)	1383:1422	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	23	118	theme	fluorescence	3332:3343	arg1	intensity					3345:3353	the fluorescence intensity	3328:3353	the fluorescence intensity of MAP3K5 in the model group	3328:3382	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	13	119	theme	cells	1511:1515	arg1	model					1477:1481	the cell model	1468:1481	the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS)	1468:1560	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	25	120	theme	ASK1/P38	3870:3877	arg1	pathway					3894:3900	the ASK1/P38 MAPK signaling pathway	3866:3900	the ASK1/P38 MAPK signaling pathway	3866:3900	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	17	121	theme	colon	2039:2043	arg1	lesions					2045:2051	colon lesions	2039:2051	colon lesions	2039:2051	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	15	122	theme	WB	1821:1822	arg1	detection					1824:1832	WB detection	1821:1832	WB detection of ASK1/P38 MAPK pathway protein expression changes.	1821:1885	WB detection of ASK1/P38 MAPK pathway protein expression changes.
36641976	23	123	theme	proteins	3440:3447	arg1	expression					3404:3413	the expression	3400:3413	the expression of ASK1/P38 MAPK pathway proteins	3400:3447	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	25	124	theme	signaling	3884:3892	arg1	pathway					3894:3900	the ASK1/P38 MAPK signaling pathway	3866:3900	the ASK1/P38 MAPK signaling pathway	3866:3900	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	6	125	theme	Colon	676:680	arg1	changes					695:701	Colon pathological changes	676:701	Colon pathological changes	676:701	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	1	126	from	medicine	214:221	arg1	China					226:230	China	226:230	China	226:230	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	127	theme	Xingpi	144:149	arg1	XP					160:161	XP	160:161	XP	160:161	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	127	theme	Xingpi	144:149	arg1	Capsule					151:157	BACKGROUND Xingpi Capsule	133:157	BACKGROUND Xingpi Capsule (XP)	133:162	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	127	theme	Xingpi	144:149	arg1	medicine					214:221	a commercially available over-the-counter herbal medicine	165:221	a commercially available over-the-counter herbal medicine in China	165:230	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	12	128	theme	RNA	1325:1327	arg1	expression					1329:1338	colon RNA expression	1319:1338	colon RNA expression	1319:1338	Changes in colon RNA expression were assessed by RNA sequencing (RNA-Seq).
36641976	0	129	theme	bowel	76:80	arg1	syndrome					82:89	diarrhea predominant-irritable bowel syndrome	45:89	diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	45:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	19	130	theme	antidiarrheal	2474:2486	arg1	effects					2488:2494	antidiarrheal effects	2474:2494	antidiarrheal effects	2474:2494	RNA-Seq combined with WB validation showed that XP exerted antidiarrheal effects by inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	13	131	theme	colonic	1492:1498	arg1	NCM460					1518:1523	NCM460	1518:1523	NCM460	1518:1523	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	13	131	theme	colonic	1492:1498	arg1	cells					1511:1515	human colonic epithelial cells	1486:1515	human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS)	1486:1560	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	25	132	theme	HPX	3814:3816	arg1	level					3805:3809	the relative expression level	3781:3809	the relative expression level of HPX, a metabolite of the microbiota	3781:3848	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	17	133	theme	severe	2137:2142	arg1	distortion					2144:2153	severe distortion	2137:2153	severe distortion	2137:2153	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	19	134	theme	MAPK	2523:2526	arg1	pathway					2538:2544	the ASK1/P38 MAPK signaling pathway	2510:2544	the ASK1/P38 MAPK signaling pathway	2510:2544	RNA-Seq combined with WB validation showed that XP exerted antidiarrheal effects by inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	3	135	dep	PURPOSE	378:384	arg1	aimed					397:401	aimed	397:401	aimed to investigate XP efficacy in IBS-D and elucidate the underlying molecular mechanisms	397:487	PURPOSE This study aimed to investigate XP efficacy in IBS-D and elucidate the underlying molecular mechanisms.
36641976	5	136	from	changes	618:624	arg1	stool					649:653	stool	649:653	stool	649:653	The changes in rat body weight and stool were recorded daily.
36641976	5	136	from	changes	618:624	arg1	weight					638:643	rat body weight	629:643	rat body weight	629:643	The changes in rat body weight and stool were recorded daily.
36641976	16	137	theme	IBS-D	1927:1931	arg1	patients					1933:1940	IBS-D patients	1927:1940	IBS-D patients	1927:1940	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	6	138	theme	goblet	729:734	arg1	cells					736:740	colonic goblet cells	721:740	colonic goblet cells of rats	721:748	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	5	139	theme	body	633:636	arg1	weight					638:643	rat body weight	629:643	rat body weight	629:643	The changes in rat body weight and stool were recorded daily.
36641976	18	140	theme	16S	2215:2217	arg1	rRNA					2219:2222	16S rRNA	2215:2222	16S rRNA gene sequence analysis	2215:2245	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	0	141	from	Capsule	34:40	arg1	treatment					91:99	diarrhea predominant-irritable bowel syndrome treatment	45:99	diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	45:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	25	142	theme	expression	3794:3803	arg1	level					3805:3809	the relative expression level	3781:3809	the relative expression level of HPX, a metabolite of the microbiota	3781:3848	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	12	143	theme	colon	1319:1323	arg1	expression					1329:1338	colon RNA expression	1319:1338	colon RNA expression	1319:1338	Changes in colon RNA expression were assessed by RNA sequencing (RNA-Seq).
36641976	4	144	theme	restraint	572:580	arg1	stress					582:587	restraint stress	572:587	restraint stress	572:587	METHODS A rat IBS-D model was established by senna decoction gavage combined with restraint stress and swimming exhaustion.
36641976	23	145	theme	LPS-induced	3186:3196	arg1	damage					3205:3210	LPS-induced NCM460 damage	3186:3210	LPS-induced NCM460 damage	3186:3210	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	1	146	theme	bowel	299:303	arg1	syndrome					305:312	diarrhea-predominant irritable bowel syndrome	268:312	diarrhea-predominant irritable bowel syndrome (IBS-D)	268:320	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	1	146	theme	bowel	299:303	arg1	IBS-D					315:319	IBS-D	315:319	IBS-D	315:319	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36641976	16	147	theme	stool	1964:1968	arg1	rate					1970:1973	the loose stool rate	1954:1973	the loose stool rate	1954:1973	RESULTS XP increased the body weight of IBS-D patients and reduced the loose stool rate, loose stool index, and Bristo score.
36641976	21	148	theme	rRNA	2732:2735	arg1	sequencing					2737:2746	16S rRNA sequencing	2728:2746	16S rRNA sequencing	2728:2746	Impressively, the correlation analysis between 16S rRNA sequencing and LC-MS suggested that HPX and Prevotella 9 are negatively correlated, which indicated that XP might increase the content of HPX by reducing the abundance of Prevotella 9.
36641976	9	149	theme	MAPK	1110:1113	arg1	pathway					1115:1121	the ASK1/P38 MAPK pathway	1097:1121	the ASK1/P38 MAPK pathway	1097:1121	Western blotting (WB) was used to analyze the protein expression of the ASK1/P38 MAPK pathway.
36641976	0	150	theme	Capsule	34:40	arg1	mechanism					14:22	the mechanism	10:22	the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	10:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	4	151	dep	METHODS	490:496	arg1	established					520:530	established	520:530	was established by senna decoction gavage combined with restraint stress and swimming exhaustion	516:611	METHODS A rat IBS-D model was established by senna decoction gavage combined with restraint stress and swimming exhaustion.
36641976	18	152	theme	gene	2224:2227	arg1	analysis					2238:2245	16S rRNA gene sequence analysis	2215:2245	16S rRNA gene sequence analysis	2215:2245	Specifically, 16S rRNA gene sequence analysis showed that XP decreased the abundance of Desulfurium and Prevotella 9 at the phylum and genus levels while increasing the abundance of Bacteroides at the genus level.
36641976	4	153	theme	decoction	541:549	arg1	gavage					551:556	senna decoction gavage	535:556	senna decoction gavage combined with restraint stress and swimming exhaustion	535:611	METHODS A rat IBS-D model was established by senna decoction gavage combined with restraint stress and swimming exhaustion.
36641976	11	154	theme	colonic	1221:1227	arg1	metabolites					1229:1239	colonic metabolites	1221:1239	colonic metabolites	1221:1239	Changes in colonic metabolites were evaluated by liquid chromatography-mass spectrometry (LC-MS).
36641976	25	155	from	protection	3696:3705	arg1	rats					3724:3727	rats	3724:3727	rats	3724:3727	CONCLUSION XP displayed multifaceted protection against IBS-D in rats by regulating the intestinal microbiota, increasing the relative expression level of HPX, a metabolite of the microbiota, and inhibiting the ASK1/P38 MAPK signaling pathway.
36641976	3	156	theme	molecular	468:476	arg1	mechanisms					478:487	the underlying molecular mechanisms	453:487	the underlying molecular mechanisms	453:487	PURPOSE This study aimed to investigate XP efficacy in IBS-D and elucidate the underlying molecular mechanisms.
36641976	0	157	theme	diarrhea	45:52	arg1	syndrome					82:89	diarrhea predominant-irritable bowel syndrome	45:89	diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology	45:130	Exploring the mechanism of Xingpi Capsule in diarrhea predominant-irritable bowel syndrome treatment based on multiomics technology.
36641976	10	158	theme	intestinal	1151:1160	arg1	microbiota					1162:1171	the rat intestinal microbiota	1143:1171	the rat intestinal microbiota	1143:1171	The composition of the rat intestinal microbiota was detected by 16S rRNA sequencing.
36641976	4	159	theme	IBS-D	504:508	arg1	model					510:514	A rat IBS-D model	498:514	A rat IBS-D model	498:514	METHODS A rat IBS-D model was established by senna decoction gavage combined with restraint stress and swimming exhaustion.
36641976	3	160	theme	XP	418:419	arg1	efficacy					421:428	XP efficacy	418:428	XP efficacy in IBS-D	418:437	PURPOSE This study aimed to investigate XP efficacy in IBS-D and elucidate the underlying molecular mechanisms.
36641976	11	161	theme	chromatography-mass	1266:1284	arg1	LC-MS					1300:1304	LC-MS	1300:1304	LC-MS	1300:1304	Changes in colonic metabolites were evaluated by liquid chromatography-mass spectrometry (LC-MS).
36641976	11	161	theme	chromatography-mass	1266:1284	arg1	spectrometry					1286:1297	liquid chromatography-mass spectrometry	1259:1297	liquid chromatography-mass spectrometry (LC-MS)	1259:1305	Changes in colonic metabolites were evaluated by liquid chromatography-mass spectrometry (LC-MS).
36641976	2	162	theme	potential	341:349	arg1	mechanisms					351:360	the potential mechanisms	337:360	the potential mechanisms	337:360	Nevertheless, the potential mechanisms remain unclear.
36641976	7	163	theme	tight-junction-associated	904:928	arg1	Occludin					892:899	Occludin	892:899	Occludin	892:899	The expression of Occludin, a tight-junction-associated protein, was examined via immunohistochemistry.
36641976	7	163	theme	tight-junction-associated	904:928	arg1	protein					930:936	a tight-junction-associated protein	902:936	a tight-junction-associated protein	902:936	The expression of Occludin, a tight-junction-associated protein, was examined via immunohistochemistry.
36641976	23	164	dep	In	3130:3131	arg1	vitro					3133:3137	vitro	3133:3137	vitro	3133:3137	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	23	165	theme	MAP3K5	3358:3363	arg1	intensity					3345:3353	the fluorescence intensity	3328:3353	the fluorescence intensity of MAP3K5 in the model group	3328:3382	In vitro experiments have proven that HPX can alleviate LPS-induced NCM460 damage, specifically manifested as enhancing cell viability, reducing cell apoptosis, increasing ZO-1 expression, reducing the fluorescence intensity of MAP3K5 in the model group, and inhibiting the expression of ASK1/P38 MAPK pathway proteins.
36641976	13	166	theme	effective	1587:1595	arg1	concentration					1597:1609	the effective concentration	1583:1609	the effective concentration of HPX	1583:1616	The nontoxic range of hypoxanthine (HPX) was screened by Cell Counting Kit-8 (CCK8), the cell model of human colonic epithelial cells (NCM460) induced by lipopolysaccharide (LPS) was established, and the effective concentration of HPX was screened by CCK8.
36641976	21	167	theme	HPX	2875:2877	arg1	content					2864:2870	the content	2860:2870	the content of HPX	2860:2877	Impressively, the correlation analysis between 16S rRNA sequencing and LC-MS suggested that HPX and Prevotella 9 are negatively correlated, which indicated that XP might increase the content of HPX by reducing the abundance of Prevotella 9.
36641976	6	168	theme	acid-Schiff	829:839	arg1	staining					850:857	Alcian blue plus periodic acid-Schiff (AB-PAS) staining	803:857	Alcian blue plus periodic acid-Schiff (AB-PAS) staining	803:857	Colon pathological changes and the number of colonic goblet cells of rats were observed by hematoxylin-eosin (HE) staining and Alcian blue plus periodic acid-Schiff (AB-PAS) staining, respectively.
36641976	21	169	theme	correlation	2699:2709	arg1	analysis					2711:2718	the correlation analysis	2695:2718	the correlation analysis between 16S rRNA sequencing and LC-MS	2695:2756	Impressively, the correlation analysis between 16S rRNA sequencing and LC-MS suggested that HPX and Prevotella 9 are negatively correlated, which indicated that XP might increase the content of HPX by reducing the abundance of Prevotella 9.
36641976	24	170	theme	downstream	3638:3647	arg1	pathways					3649:3656	its downstream pathways	3634:3656	its downstream pathways	3634:3656	The protective effect of HPX was reversed after transfection with pcDNA 3.1-MAP3K5, which fully demonstrated that the protective mechanism of HPX was achieved by inhibiting MAP3K5 and its downstream pathways.
36641976	17	171	theme	cells	2085:2089	arg1	expression					2099:2108	the expression	2095:2108	the expression of Occludin	2095:2120	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	17	171	theme	cells	2085:2089	arg1	number					2068:2073	the number	2064:2073	the number of goblet cells	2064:2089	In addition, XP mitigated colon lesions, increased the number of goblet cells and the expression of Occludin, and prevented severe distortion and effacement of the microvillous structure.
36641976	1	172	theme	prominent	241:249	arg1	role					251:254	a prominent role	239:254	a prominent role	239:254	BACKGROUND Xingpi Capsule (XP), a commercially available over-the-counter herbal medicine in China, plays a prominent role in treating diarrhea-predominant irritable bowel syndrome (IBS-D).
36256565	13	0	theme	fatty	1539:1543	arg1	acids					1545:1549	The major fatty acids	1529:1549	The major fatty acids	1529:1549	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	0	theme	fatty	1539:1543	arg1	feature					1638:1644	summed feature 8	1631:1646	summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c)	1631:1679	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	0	theme	fatty	1539:1543	arg1	feature					1584:1590	C16:0, summed feature 3	1570:1592	feature	1584:1590	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	9	1	theme	tRNA	1171:1174	arg1	genes					1161:1165	4064 protein-coding genes	1141:1165	4064 protein-coding genes	1141:1165	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	9	1	theme	tRNA	1171:1174	arg1	genes					1176:1180	60 tRNA genes	1168:1180	60 tRNA genes	1168:1180	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	14	2	theme	novel	1811:1815	arg1	species					1817:1823	a novel species	1809:1823	a novel species	1809:1823	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	5	3	theme	MacConkey	537:545	arg1	agar					547:550	MacConkey agar	537:550	MacConkey agar	537:550	Cell growth occurred on tryptone soya agar, Luria-Bertani agar, NA, MacConkey agar and Reasoner's 2A agar.
36256565	8	4	dep	rRNA	737:740	arg1	comparisons					756:766	gene sequence comparisons	742:766	the 16S rRNA gene sequence comparisons	729:766	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	5	theme	18195	980:984	arg1	T					986:986	Pseudomonas sagittaria JCM 18195 T	953:986	Pseudomonas sagittaria JCM 18195 T (97.6 %)	953:995	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	5	theme	18195	980:984	arg1	%					994:994	97.6 %	989:994	97.6 %	989:994	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	12	6	theme	predominant	1480:1490	arg1	ubiquinone					1515:1524	ubiquinone 9	1515:1526	ubiquinone 9	1515:1526	The predominant isoprenoid quinone was ubiquinone 9.
36256565	12	6	theme	predominant	1480:1490	arg1	quinone					1503:1509	The predominant isoprenoid quinone	1476:1509	The predominant isoprenoid quinone	1476:1509	The predominant isoprenoid quinone was ubiquinone 9.
36256565	6	7	dep	oxidase	621:627	arg1	tests					642:646	tests	642:646	tests	642:646	The strain was found to be positive for both oxidase and catalase tests.
36256565	10	8	theme	strain	1297:1302	arg1	MAHUQ-58T					1304:1312	strain MAHUQ-58T	1297:1312	strain MAHUQ-58T	1297:1312	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	2	9	theme	novel	122:126	arg1	strain					138:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	7	10	theme	Tween	691:695	arg1	hydrolysis					677:686	hydrolysis	677:686	hydrolysis of Tween 20 and l-tyrosine	677:713	The strain was positive for hydrolysis of Tween 20 and l-tyrosine.
36256565	9	11	theme	rRNA	1191:1194	arg1	genes					1196:1200	four rRNA genes	1186:1200	four rRNA genes	1186:1200	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	9	11	theme	rRNA	1191:1194	arg1	genes					1161:1165	4064 protein-coding genes	1141:1165	4064 protein-coding genes	1141:1165	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	8	12	theme	Pseudomonas	821:831	arg1	member					801:806	a member	799:806	a member of the genus Pseudomonas	799:831	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	12	theme	Pseudomonas	821:831	arg1	isolate					773:779	the isolate	769:779	the isolate	769:779	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	10	13	theme	type	1339:1342	arg1	strains					1344:1350	four closely related type strains	1318:1350	four closely related type strains	1318:1350	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	14	14	dep	name	1869:1872	arg1	sp					1895:1896	Pseudomonas oryzagri sp	1874:1896	the name Pseudomonas oryzagri sp	1865:1896	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	8	15	dep	Pseudomonas	908:918	arg1	linyingensis					920:931	linyingensis	920:931	linyingensis	920:931	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	15	16	theme	type	1969:1972	arg1	strain					1974:1979	the type strain	1965:1979	the type strain	1965:1979	nov. is proposed, with MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain.
36256565	13	17	dep	feature	1638:1644	arg1	C18 					1649:1652	C18 	1649:1652	C18 	1649:1652	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	11	18	theme	67.0 mol	1465:1472	arg1	content					1436:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	11	18	theme	67.0 mol	1465:1472	arg1	%					1473:1473	67.0 mol%	1465:1473	67.0 mol%	1465:1473	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	15	19	theme	=KACC	1933:1937	arg1	1.18518T					1952:1959	=KACC 22005T=CGMCC 1.18518T	1933:1959	=KACC 22005T=CGMCC 1.18518T	1933:1959	nov. is proposed, with MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain.
36256565	15	19	theme	=KACC	1933:1937	arg1	MAHUQ-58T					1922:1930	MAHUQ-58T	1922:1930	MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain	1922:1979	nov. is proposed, with MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain.
36256565	4	20	theme	Strain	366:371	arg1	MAHUQ-58T					373:381	Strain MAHUQ-58T	366:381	Strain MAHUQ-58T	366:381	Strain MAHUQ-58T was found to be able to grow at 15-40 °C, at pH 5.5-10.0 and with 0-1.0 % NaCl (w/v).
36256565	9	21	theme	strain	1055:1060	arg1	MAHUQ-58T					1062:1070	The novel strain MAHUQ-58T	1045:1070	The novel strain MAHUQ-58T	1045:1070	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	2	22	theme	field	208:212	arg1	sample					191:196	soil sample	186:196	soil sample of a rice field	186:212	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	14	23	theme	genotypic	1719:1727	arg1	results					1729:1735	genotypic results	1719:1735	genotypic results	1719:1735	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	9	24	theme	draft	1078:1082	arg1	size					1091:1094	a draft genome size	1076:1094	a draft genome size	1076:1094	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	5	25	theme	tryptone	493:500	arg1	agar					507:510	tryptone soya agar	493:510	tryptone soya agar	493:510	Cell growth occurred on tryptone soya agar, Luria-Bertani agar, NA, MacConkey agar and Reasoner's 2A agar.
36256565	1	26	theme	rice	47:50	arg1	soil					58:61	a rice field soil	45:61	a rice field soil	45:61	nov., isolated from a rice field soil.
36256565	10	27	theme	identity	1226:1233	arg1	values					1282:1287	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1203:1287	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains	1203:1350	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	9	28	contain	has	1072:1074	arg2	size					1091:1094	a draft genome size	1076:1094	a draft genome size	1076:1094	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	9	28	contain	has	1072:1074	arg1	MAHUQ-58T					1062:1070	The novel strain MAHUQ-58T	1045:1070	The novel strain MAHUQ-58T	1045:1070	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	10	29	theme	 and	1384:1387	arg1	%					1399:1399	85.5-89.5 % and 29.5-38.0 %	1373:1399	85.5-89.5 % and 29.5-38.0 %	1373:1399	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	3	30	theme	agar	337:340	arg1	medium					347:352	nutrient agar (NA) medium	328:352	nutrient agar (NA) medium for 2 days	328:363	The colonies were observed to be light pink-coloured, smooth, spherical and 0.6-1.0 mm in diameter when grown on nutrient agar (NA) medium for 2 days.
36256565	10	31	theme	average	1207:1213	arg1	ANI					1236:1238	ANI	1236:1238	ANI	1236:1238	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	10	31	theme	average	1207:1213	arg1	identity					1226:1233	average nucleotide identity	1207:1233	average nucleotide identity (ANI)	1207:1239	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	10	32	theme	85.5-89.5 	1373:1382	arg1	%					1399:1399	85.5-89.5 % and 29.5-38.0 %	1373:1399	85.5-89.5 % and 29.5-38.0 %	1373:1399	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	0	33	theme	Pseudomonas	0:10	arg1	sp					21:22	Pseudomonas oryzagri sp	0:22	Pseudomonas oryzagri sp.	0:23	Pseudomonas oryzagri sp.
36256565	9	34	theme	4 536 129 bp	1099:1110	arg1	size					1091:1094	a draft genome size	1076:1094	a draft genome size	1076:1094	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	15	35	theme	22005T=CGMCC	1939:1950	arg1	1.18518T					1952:1959	=KACC 22005T=CGMCC 1.18518T	1933:1959	=KACC 22005T=CGMCC 1.18518T	1933:1959	nov. is proposed, with MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain.
36256565	15	35	theme	22005T=CGMCC	1939:1950	arg1	MAHUQ-58T					1922:1930	MAHUQ-58T	1922:1930	MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain	1922:1979	nov. is proposed, with MAHUQ-58T (=KACC 22005T=CGMCC 1.18518T) as the type strain.
36256565	5	36	theme	Cell	469:472	arg1	growth					474:479	Cell growth	469:479	Cell growth	469:479	Cell growth occurred on tryptone soya agar, Luria-Bertani agar, NA, MacConkey agar and Reasoner's 2A agar.
36256565	2	37	attach	isolated	172:179	arg2	strain					138:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	2	37	attach	isolated	172:179	arg1	sample					191:196	soil sample	186:196	soil sample of a rice field	186:212	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	4	38	theme	%	455:455	arg1	 NaCl					456:460	0-1.0 % NaCl	449:460	0-1.0 % NaCl (w/v)	449:466	Strain MAHUQ-58T was found to be able to grow at 15-40 °C, at pH 5.5-10.0 and with 0-1.0 % NaCl (w/v).
36256565	4	38	theme	%	455:455	arg1	w/v					463:465	w/v	463:465	w/v	463:465	Strain MAHUQ-58T was found to be able to grow at 15-40 °C, at pH 5.5-10.0 and with 0-1.0 % NaCl (w/v).
36256565	8	39	theme	%	893:893	arg1	WM-3T					881:885	Pseudomonas oryzae WM-3T	862:885	Pseudomonas oryzae WM-3T (98.9 % similarity)	862:905	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	39	theme	%	893:893	arg1	similarity					895:904	98.9 % similarity	888:904	98.9 % similarity	888:904	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	40	theme	gene	742:745	arg1	comparisons					756:766	gene sequence comparisons	742:766	the 16S rRNA gene sequence comparisons	729:766	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	41	theme	sequence	747:754	arg1	comparisons					756:766	gene sequence comparisons	742:766	the 16S rRNA gene sequence comparisons	729:766	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	11	42	theme	DNA	1428:1430	arg1	content					1436:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	11	42	theme	DNA	1428:1430	arg1	%					1473:1473	67.0 mol%	1465:1473	67.0 mol%	1465:1473	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	10	43	theme	%	1399:1399	arg1	range					1364:1368	the range	1360:1368	the range of 85.5-89.5 % and 29.5-38.0 %, respectively	1360:1413	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	3	44	theme	NA	343:344	arg1	medium					347:352	nutrient agar (NA) medium	328:352	nutrient agar (NA) medium for 2 days	328:363	The colonies were observed to be light pink-coloured, smooth, spherical and 0.6-1.0 mm in diameter when grown on nutrient agar (NA) medium for 2 days.
36256565	8	45	theme	Pseudomonas	908:918	arg1	LYBRD3-7T					933:941	Pseudomonas linyingensis LYBRD3-7T	908:941	Pseudomonas linyingensis LYBRD3-7T (97.7 %)	908:950	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	45	theme	Pseudomonas	908:918	arg1	%					949:949	97.7 %	944:949	97.7 %	944:949	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	10	46	theme	DNA-DNA	1253:1259	arg1	dDDH					1276:1279	dDDH	1276:1279	dDDH	1276:1279	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	10	46	theme	DNA-DNA	1253:1259	arg1	hybridization					1261:1273	digital DNA-DNA hybridization	1245:1273	digital DNA-DNA hybridization (dDDH)	1245:1280	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	13	47	theme	major	1533:1537	arg1	acids					1545:1549	The major fatty acids	1529:1549	The major fatty acids	1529:1549	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	47	theme	major	1533:1537	arg1	feature					1638:1644	summed feature 8	1631:1646	summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c)	1631:1679	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	47	theme	major	1533:1537	arg1	feature					1584:1590	C16:0, summed feature 3	1570:1592	feature	1584:1590	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	2	48	theme	rod-shaped	96:105	arg1	strain					138:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	14	49	dep	dDDH	1698:1701	arg1	the					1685:1687	the	1685:1687	the	1685:1687	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	14	49	dep	dDDH	1698:1701	arg1	basis					1689:1693	basis	1689:1693	basis	1689:1693	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	8	50	dep	Pseudomonas	862:872	arg1	oryzae					874:879	oryzae	874:879	oryzae	874:879	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	2	51	theme	aerobic	87:93	arg1	strain					138:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	13	52	theme	summed	1631:1636	arg1	acids					1545:1549	The major fatty acids	1529:1549	The major fatty acids	1529:1549	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	52	theme	summed	1631:1636	arg1	feature					1638:1644	summed feature 8	1631:1646	summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c)	1631:1679	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	8	53	theme	Pseudomonas	953:963	arg1	T					986:986	Pseudomonas sagittaria JCM 18195 T	953:986	Pseudomonas sagittaria JCM 18195 T (97.6 %)	953:995	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	53	theme	Pseudomonas	953:963	arg1	%					994:994	97.6 %	989:994	97.6 %	989:994	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	12	54	theme	isoprenoid	1492:1501	arg1	ubiquinone					1515:1524	ubiquinone 9	1515:1526	ubiquinone 9	1515:1526	The predominant isoprenoid quinone was ubiquinone 9.
36256565	12	54	theme	isoprenoid	1492:1501	arg1	quinone					1503:1509	The predominant isoprenoid quinone	1476:1509	The predominant isoprenoid quinone	1476:1509	The predominant isoprenoid quinone was ubiquinone 9.
36256565	2	55	theme	bacterial	128:136	arg1	strain					138:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	7	56	theme	l-tyrosine	704:713	arg1	hydrolysis					677:686	hydrolysis	677:686	hydrolysis of Tween 20 and l-tyrosine	677:713	The strain was positive for hydrolysis of Tween 20 and l-tyrosine.
36256565	8	57	theme	JCM	976:978	arg1	T					986:986	Pseudomonas sagittaria JCM 18195 T	953:986	Pseudomonas sagittaria JCM 18195 T (97.6 %)	953:995	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	57	theme	JCM	976:978	arg1	%					994:994	97.6 %	989:994	97.6 %	989:994	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	13	58	dep	C16 	1595:1598	arg1	ω7c					1622:1624	ω7c	1622:1624	ω7c	1622:1624	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	58	dep	C16 	1595:1598	arg1	ω6c					1603:1605	ω6c	1603:1605	ω6c	1603:1605	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	58	dep	C16 	1595:1598	arg1	C16 					1614:1617	C16 	1614:1617	C16 	1614:1617	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	14	59	theme	strain	1781:1786	arg1	MAHUQ-58T					1788:1796	strain MAHUQ-58T	1781:1796	strain MAHUQ-58T	1781:1796	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	2	60	theme	non-motile	111:120	arg1	strain					138:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	5	61	theme	Luria-Bertani	513:525	arg1	agar					527:530	Luria-Bertani agar	513:530	Luria-Bertani agar	513:530	Cell growth occurred on tryptone soya agar, Luria-Bertani agar, NA, MacConkey agar and Reasoner's 2A agar.
36256565	10	62	theme	related	1331:1337	arg1	strains					1344:1350	four closely related type strains	1318:1350	four closely related type strains	1318:1350	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	5	63	theme	2A	567:568	arg1	agar					570:573	Reasoner's 2A agar	556:573	Reasoner's 2A agar	556:573	Cell growth occurred on tryptone soya agar, Luria-Bertani agar, NA, MacConkey agar and Reasoner's 2A agar.
36256565	14	64	theme	physiological	1761:1773	arg1	data					1775:1778	chemotaxonomic and physiological data	1742:1778	chemotaxonomic and physiological data	1742:1778	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	3	65	from	diameter	305:312	arg1	pink-coloured					254:266	pink-coloured	254:266	pink-coloured	254:266	The colonies were observed to be light pink-coloured, smooth, spherical and 0.6-1.0 mm in diameter when grown on nutrient agar (NA) medium for 2 days.
36256565	3	65	from	diameter	305:312	arg1	colonies					219:226	The colonies	215:226	The colonies	215:226	The colonies were observed to be light pink-coloured, smooth, spherical and 0.6-1.0 mm in diameter when grown on nutrient agar (NA) medium for 2 days.
36256565	2	66	theme	rice	203:206	arg1	field					208:212	a rice field	201:212	a rice field	201:212	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	14	67	theme	chemotaxonomic	1742:1755	arg1	data					1775:1778	chemotaxonomic and physiological data	1742:1778	chemotaxonomic and physiological data	1742:1778	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	2	68	theme	Gram-stain-negative	66:84	arg1	strain					138:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain	64:143	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	8	69	theme	Pseudomonas	1001:1011	arg1	SgZ-6T					1028:1033	Pseudomonas guangdongensis SgZ-6T	1001:1033	Pseudomonas guangdongensis SgZ-6T (97.2 %)	1001:1042	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	69	theme	Pseudomonas	1001:1011	arg1	%					1041:1041	97.2 %	1036:1041	97.2 %	1036:1041	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	9	70	theme	novel	1049:1053	arg1	MAHUQ-58T					1062:1070	The novel strain MAHUQ-58T	1045:1070	The novel strain MAHUQ-58T	1045:1070	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	8	71	theme	Pseudomonas	862:872	arg1	WM-3T					881:885	Pseudomonas oryzae WM-3T	862:885	Pseudomonas oryzae WM-3T (98.9 % similarity)	862:905	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	71	theme	Pseudomonas	862:872	arg1	similarity					895:904	98.9 % similarity	888:904	98.9 % similarity	888:904	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	72	dep	Pseudomonas	953:963	arg1	sagittaria					965:974	sagittaria	965:974	sagittaria	965:974	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	13	73	theme	C16:0	1570:1574	arg1	acids					1545:1549	The major fatty acids	1529:1549	The major fatty acids	1529:1549	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	73	theme	C16:0	1570:1574	arg1	feature					1584:1590	C16:0, summed feature 3	1570:1592	feature	1584:1590	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	9	74	theme	genome	1084:1089	arg1	size					1091:1094	a draft genome size	1076:1094	a draft genome size	1076:1094	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	5	75	theme	soya	502:505	arg1	agar					507:510	tryptone soya agar	493:510	tryptone soya agar	493:510	Cell growth occurred on tryptone soya agar, Luria-Bertani agar, NA, MacConkey agar and Reasoner's 2A agar.
36256565	1	76	theme	field	52:56	arg1	soil					58:61	a rice field soil	45:61	a rice field soil	45:61	nov., isolated from a rice field soil.
36256565	14	77	dep	genus	1836:1840	arg1	Pseudomonas					1842:1852	the genus Pseudomonas	1832:1852	the genus Pseudomonas	1832:1852	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	10	78	theme	nucleotide	1215:1224	arg1	ANI					1236:1238	ANI	1236:1238	ANI	1236:1238	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	10	78	theme	nucleotide	1215:1224	arg1	identity					1226:1233	average nucleotide identity	1207:1233	average nucleotide identity (ANI)	1207:1239	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	3	79	from	pink-coloured	254:266	arg1	diameter					305:312	diameter	305:312	diameter	305:312	The colonies were observed to be light pink-coloured, smooth, spherical and 0.6-1.0 mm in diameter when grown on nutrient agar (NA) medium for 2 days.
36256565	13	80	theme	summed	1577:1582	arg1	acids					1545:1549	The major fatty acids	1529:1549	The major fatty acids	1529:1549	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	80	theme	summed	1577:1582	arg1	feature					1584:1590	C16:0, summed feature 3	1570:1592	feature	1584:1590	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	10	81	theme	%	1383:1383	arg1	%					1399:1399	85.5-89.5 % and 29.5-38.0 %	1373:1399	85.5-89.5 % and 29.5-38.0 %	1373:1399	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	0	82	theme	oryzagri	12:19	arg1	sp					21:22	Pseudomonas oryzagri sp	0:22	Pseudomonas oryzagri sp.	0:23	Pseudomonas oryzagri sp.
36256565	8	83	theme	98.9 	888:892	arg1	WM-3T					881:885	Pseudomonas oryzae WM-3T	862:885	Pseudomonas oryzae WM-3T (98.9 % similarity)	862:905	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	83	theme	98.9 	888:892	arg1	similarity					895:904	98.9 % similarity	888:904	98.9 % similarity	888:904	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	8	84	theme	16S	733:735	arg1	rRNA					737:740	the 16S rRNA gene sequence comparisons	729:766	the 16S rRNA gene sequence comparisons	729:766	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	3	85	theme	nutrient	328:335	arg1	medium					347:352	nutrient agar (NA) medium	328:352	nutrient agar (NA) medium for 2 days	328:363	The colonies were observed to be light pink-coloured, smooth, spherical and 0.6-1.0 mm in diameter when grown on nutrient agar (NA) medium for 2 days.
36256565	8	86	dep	Pseudomonas	1001:1011	arg1	guangdongensis					1013:1026	guangdongensis	1013:1026	guangdongensis	1013:1026	According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Pseudomonas and to be closely related to Pseudomonas oryzae WM-3T (98.9 % similarity), Pseudomonas linyingensis LYBRD3-7T (97.7 %), Pseudomonas sagittaria JCM 18195 T (97.6 %) and Pseudomonas guangdongensis SgZ-6T (97.2 %).
36256565	11	87	theme	genomic	1420:1426	arg1	content					1436:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	11	87	theme	genomic	1420:1426	arg1	%					1473:1473	67.0 mol%	1465:1473	67.0 mol%	1465:1473	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	14	88	theme	oryzagri	1886:1893	arg1	sp					1895:1896	Pseudomonas oryzagri sp	1874:1896	the name Pseudomonas oryzagri sp	1865:1896	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	13	89	dep	C18 	1649:1652	arg1	C18 					1668:1671	C18 	1668:1671	C18 	1668:1671	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	89	dep	C18 	1649:1652	arg1	ω7c					1676:1678	ω7c	1676:1678	ω7c	1676:1678	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	89	dep	C18 	1649:1652	arg1	ω6c					1657:1659	ω6c	1657:1659	ω6c	1657:1659	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	13	90	dep	feature	1584:1590	arg1	C16 					1595:1598	C16 	1595:1598	C16 	1595:1598	The major fatty acids were identified as C16:0, summed feature 3 (C16 : 1 ω6c and/or C16 : 1 ω7c) and summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c).
36256565	4	91	theme	0-1.0 	449:454	arg1	 NaCl					456:460	0-1.0 % NaCl	449:460	0-1.0 % NaCl (w/v)	449:466	Strain MAHUQ-58T was found to be able to grow at 15-40 °C, at pH 5.5-10.0 and with 0-1.0 % NaCl (w/v).
36256565	4	91	theme	0-1.0 	449:454	arg1	w/v					463:465	w/v	463:465	w/v	463:465	Strain MAHUQ-58T was found to be able to grow at 15-40 °C, at pH 5.5-10.0 and with 0-1.0 % NaCl (w/v).
36256565	10	92	theme	hybridization	1261:1273	arg1	values					1282:1287	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1203:1287	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains	1203:1350	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	2	93	theme	soil	186:189	arg1	sample					191:196	soil sample	186:196	soil sample of a rice field	186:212	A Gram-stain-negative, aerobic, rod-shaped and non-motile novel bacterial strain, designated MAHUQ-58T, was isolated from soil sample of a rice field.
36256565	14	94	theme	ANI	1707:1709	arg1	values					1711:1716	ANI values	1707:1716	ANI values	1707:1716	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	14	95	theme	Pseudomonas	1874:1884	arg1	sp					1895:1896	Pseudomonas oryzagri sp	1874:1896	the name Pseudomonas oryzagri sp	1865:1896	On the basis of dDDH and ANI values, genotypic results, and chemotaxonomic and physiological data, strain MAHUQ-58T represents a novel species within the genus Pseudomonas, for which the name Pseudomonas oryzagri sp.
36256565	10	96	theme	digital	1245:1251	arg1	dDDH					1276:1279	dDDH	1276:1279	dDDH	1276:1279	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	10	96	theme	digital	1245:1251	arg1	hybridization					1261:1273	digital DNA-DNA hybridization	1245:1273	digital DNA-DNA hybridization (dDDH)	1245:1280	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain MAHUQ-58T and four closely related type strains were in the range of 85.5-89.5 % and 29.5-38.0 %, respectively.
36256565	11	97	theme	G+C	1432:1434	arg1	content					1436:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content	1416:1442	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	11	97	theme	G+C	1432:1434	arg1	%					1473:1473	67.0 mol%	1465:1473	67.0 mol%	1465:1473	The genomic DNA G+C content was determined to be 67.0 mol%.
36256565	9	98	theme	protein-coding	1146:1159	arg1	genes					1161:1165	4064 protein-coding genes	1141:1165	4064 protein-coding genes	1141:1165	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	9	98	theme	protein-coding	1146:1159	arg1	genes					1196:1200	four rRNA genes	1186:1200	four rRNA genes	1186:1200	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
36256565	9	98	theme	protein-coding	1146:1159	arg1	genes					1176:1180	60 tRNA genes	1168:1180	60 tRNA genes	1168:1180	The novel strain MAHUQ-58T has a draft genome size of 4 536 129 bp (46 contigs), annotated with 4064 protein-coding genes, 60 tRNA genes and four rRNA genes.
35267889	1	0	theme	cement-based	142:153	arg1	composites					155:164	cement-based composites	142:164	cement-based composites	142:164	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	4	1	theme	cement	573:578	arg1	hydration					580:588	cement hydration	573:588	cement hydration	573:588	Incorporating KGM could significantly prolong cement hydration without strength damage.
35267889	0	2	theme	Cement	71:76	arg1	Hydration					58:66	the Early Hydration	48:66	the Early Hydration of Cement	48:76	Konjac Glucomannan Induced Retarding Effects on the Early Hydration of Cement.
35267889	1	3	theme	composites	155:164	arg1	retarders					92:100	retarders	92:100	retarders	92:100	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	1	3	theme	composites	155:164	arg1	regulators					128:137	the setting time regulators	111:137	the setting time regulators of cement-based composites	111:164	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	3	4	theme	cement	519:524	arg1	hydration					488:496	the hydration	484:496	the hydration of ordinary Portland cement	484:524	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	5	5	theme	hydration	873:881	arg1	process					855:861	the process	851:861	the process of cement hydration	851:881	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	3	6	from	hydration	488:496	arg1	mechanism					457:465	the mechanism	453:465	the mechanism of its effect on the hydration of ordinary Portland cement	453:524	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	5	7	theme	hydration	908:916	arg1	heat					900:903	the heat	896:903	the heat of hydration	896:916	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	5	8	theme	clinker	806:812	arg1	surface					788:794	the surface	784:794	the surface of cement clinker	784:812	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	2	9	theme	setting	267:273	arg1	time					275:278	the setting time	263:278	the setting time	263:278	However, the limited ability to adjust the setting time restricts the application of polysaccharides in special environments.
35267889	5	10	theme	cement	866:871	arg1	hydration					873:881	cement hydration	866:881	cement hydration	866:881	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	2	11	dep	ability	245:251	arg1	adjust					256:261	adjust	256:261	to adjust the setting time	253:278	However, the limited ability to adjust the setting time restricts the application of polysaccharides in special environments.
35267889	5	12	theme	cement	712:717	arg1	hydration					719:727	cement hydration	712:727	cement hydration	712:727	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	6	13	theme	study	940:944	arg1	critical					950:957	critical	950:957	critical	950:957	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	6	13	theme	study	940:944	arg1	findings					923:930	The findings	919:930	The findings of this study	919:944	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	3	14	from	mechanism	457:465	arg1	hydration					488:496	the hydration	484:496	the hydration of ordinary Portland cement	484:524	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	3	15	theme	Portland	510:517	arg1	cement					519:524	ordinary Portland cement	501:524	ordinary Portland cement	501:524	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	0	16	from	Effects	37:43	arg1	Hydration					58:66	the Early Hydration	48:66	the Early Hydration of Cement	48:76	Konjac Glucomannan Induced Retarding Effects on the Early Hydration of Cement.
35267889	5	17	theme	active	632:637	arg1	group					648:652	the active hydroxyl group	628:652	the active hydroxyl group (-OH, rich in KGM)	628:671	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	2	18	theme	polysaccharides	309:323	arg1	application					294:304	the application	290:304	the application of polysaccharides in special environments	290:347	However, the limited ability to adjust the setting time restricts the application of polysaccharides in special environments.
35267889	6	19	theme	composite	1021:1029	arg1	materials					1031:1039	polysaccharide-cement-based composite materials	993:1039	polysaccharide-cement-based composite materials for application in various special environments	993:1087	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	0	20	theme	Konjac	0:5	arg1	Glucomannan					7:17	Konjac Glucomannan	0:17	Konjac Glucomannan	0:17	Konjac Glucomannan Induced Retarding Effects on the Early Hydration of Cement.
35267889	5	21	theme	hydroxyl	639:646	arg1	group					648:652	the active hydroxyl group	628:652	the active hydroxyl group (-OH, rich in KGM)	628:671	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	6	22	theme	polysaccharide-cement-based	993:1019	arg1	materials					1031:1039	polysaccharide-cement-based composite materials	993:1039	polysaccharide-cement-based composite materials for application in various special environments	993:1087	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	0	23	theme	Retarding	27:35	arg1	Effects					37:43	Retarding Effects	27:43	Retarding Effects on the Early Hydration of Cement	27:76	Konjac Glucomannan Induced Retarding Effects on the Early Hydration of Cement.
35267889	4	24	theme	Incorporating	527:539	arg1	KGM					541:543	Incorporating KGM	527:543	Incorporating KGM	527:543	Incorporating KGM could significantly prolong cement hydration without strength damage.
35267889	3	25	from	effect	474:479	arg1	hydration					488:496	the hydration	484:496	the hydration of ordinary Portland cement	484:524	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	3	26	theme	ordinary	501:508	arg1	cement					519:524	ordinary Portland cement	501:524	ordinary Portland cement	501:524	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	1	27	theme	various	189:195	arg1	environments					210:221	various construction environments	189:221	various construction environments	189:221	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	1	28	theme	construction	197:208	arg1	environments					210:221	various construction environments	189:221	various construction environments	189:221	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	3	29	theme	konjac	415:420	arg1	KGM					435:437	KGM	435:437	KGM	435:437	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	3	29	theme	konjac	415:420	arg1	retarder					405:412	a naturally high-efficiency retarder	377:412	a naturally high-efficiency retarder	377:412	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	3	29	theme	konjac	415:420	arg1	glucomannan					422:432	konjac glucomannan	415:432	konjac glucomannan (KGM)	415:438	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	5	30	theme	cross-linking	740:752	arg1	network					754:760	a cross-linking network	738:760	a cross-linking network	738:760	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	5	31	dep	group	648:652	arg1	-OH					655:657	-OH	655:657	-OH	655:657	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	1	32	theme	environments	210:221	arg1	demands					178:184	the demands	174:184	the demands of various construction environments	174:221	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	0	33	theme	Early	52:56	arg1	Hydration					58:66	the Early Hydration	48:66	the Early Hydration of Cement	48:76	Konjac Glucomannan Induced Retarding Effects on the Early Hydration of Cement.
35267889	5	34	theme	rich	660:663	arg1	-OH					655:657	-OH	655:657	-OH	655:657	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	4	35	theme	strength	598:605	arg1	damage					607:612	strength damage	598:612	strength damage	598:612	Incorporating KGM could significantly prolong cement hydration without strength damage.
35267889	6	36	theme	ongoing	966:972	arg1	efforts					974:980	the ongoing efforts	962:980	the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments	962:1087	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	3	37	theme	effect	474:479	arg1	mechanism					457:465	the mechanism	453:465	the mechanism of its effect on the hydration of ordinary Portland cement	453:524	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	1	38	theme	setting	115:121	arg1	retarders					92:100	retarders	92:100	retarders	92:100	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	1	38	theme	setting	115:121	arg1	regulators					128:137	the setting time regulators	111:137	the setting time regulators of cement-based composites	111:164	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	5	39	from	KGM	668:670	arg1	rich					660:663	rich	660:663	rich	660:663	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	6	40	dep	efforts	974:980	arg1	develop					985:991	develop	985:991	to develop polysaccharide-cement-based composite materials for application in various special environments	982:1087	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	5	41	attach	released	698:705	arg2	Ca2+					692:695	Ca2+	692:695	Ca2+ (released from cement hydration)	692:728	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	5	41	attach	released	698:705	arg1	hydration					719:727	cement hydration	712:727	cement hydration	712:727	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	1	42	theme	time	123:126	arg1	retarders					92:100	retarders	92:100	retarders	92:100	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	1	42	theme	time	123:126	arg1	regulators					128:137	the setting time regulators	111:137	the setting time regulators of cement-based composites	111:164	Customarily, retarders serve as the setting time regulators of cement-based composites to meet the demands of various construction environments.
35267889	2	43	theme	special	328:334	arg1	environments					336:347	special environments	328:347	special environments	328:347	However, the limited ability to adjust the setting time restricts the application of polysaccharides in special environments.
35267889	5	44	theme	cement	799:804	arg1	clinker					806:812	cement clinker	799:812	cement clinker	799:812	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	6	45	from	application	1045:1055	arg1	environments					1076:1087	various special environments	1060:1087	various special environments	1060:1087	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	6	46	theme	special	1068:1074	arg1	environments					1076:1087	various special environments	1060:1087	various special environments	1060:1087	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	5	47	from	rich	660:663	arg1	KGM					668:670	KGM	668:670	KGM	668:670	Furthermore, the active hydroxyl group (-OH, rich in KGM) could chelate with Ca2+ (released from cement hydration) to form a cross-linking network, which is adsorbed on the surface of cement clinker, thereby being conducive to delaying the process of cement hydration and reducing the heat of hydration.
35267889	3	48	theme	high-efficiency	389:403	arg1	retarder					405:412	a naturally high-efficiency retarder	377:412	a naturally high-efficiency retarder	377:412	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	3	48	theme	high-efficiency	389:403	arg1	glucomannan					422:432	konjac glucomannan	415:432	konjac glucomannan (KGM)	415:438	In this study, we reported a naturally high-efficiency retarder, konjac glucomannan (KGM), and studied the mechanism of its effect on the hydration of ordinary Portland cement.
35267889	2	49	theme	limited	237:243	arg1	ability					245:251	the limited ability	233:251	the limited ability to adjust the setting time	233:278	However, the limited ability to adjust the setting time restricts the application of polysaccharides in special environments.
35267889	6	50	theme	various	1060:1066	arg1	environments					1076:1087	various special environments	1060:1087	various special environments	1060:1087	The findings of this study are critical to the ongoing efforts to develop polysaccharide-cement-based composite materials for application in various special environments.
35267889	2	51	from	application	294:304	arg1	environments					336:347	special environments	328:347	special environments	328:347	However, the limited ability to adjust the setting time restricts the application of polysaccharides in special environments.
36603585	6	0	theme	asthma	914:919	arg1	odds					921:924	3-fold higher asthma odds	900:924	3-fold higher asthma odds	900:924	FINDINGS Children who took antibiotics without breastfeeding had 3-fold higher asthma odds, whereas there was no such association in children who received antibiotics while breastfeeding.
36603585	7	1	theme	infant	1128:1133	arg1	microbiome					1135:1144	the infant microbiome	1124:1144	the infant microbiome	1124:1144	This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome.
36603585	10	2	theme	broader	1446:1452	arg1	changes					1465:1471	these broader functional changes	1440:1471	these broader functional changes	1440:1471	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	12	3	theme	Canada	1859:1864	arg1	Institutes					1752:1761	the Canadian Institutes	1739:1761	the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia	1739:1893	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	2	4	theme	Early	146:150	arg1	exposure					163:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	4	5	from	influence	482:490	arg1	risk					541:544	antibiotic-associated asthma risk	512:544	antibiotic-associated asthma risk in a subset of children (n = 2,521)	512:580	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	11	6	theme	antibiotic-associated	1661:1681	arg1	risk					1690:1693	reduced antibiotic-associated asthma risk	1653:1693	reduced antibiotic-associated asthma risk	1653:1693	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	7	7	theme	taxonomic	1085:1093	arg1	components					1110:1119	taxonomic and functional components	1085:1119	taxonomic and functional components of the infant microbiome	1085:1144	This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome.
36603585	8	8	theme	enriched	1215:1222	arg1	subsp					1247:1251	enriched Bifidobacterium longum subsp	1215:1251	enriched Bifidobacterium longum subsp	1215:1251	Functional changes associated with asthma protection were linked to enriched Bifidobacterium longum subsp.
36603585	10	9	from	selection	1307:1315	arg1	samples					1379:1385	paired maternal samples	1363:1385	paired maternal samples that were positively associated with B. infantis and these broader functional changes	1363:1471	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	5	10	theme	metagenomic	679:689	arg1	sequencing					691:700	shotgun metagenomic sequencing	671:700	shotgun metagenomic sequencing	671:700	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	11	11	theme	opposing	1543:1550	arg1	effects					1552:1558	opposing effects	1543:1558	opposing effects	1543:1558	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	8	12	theme	longum	1240:1245	arg1	subsp					1247:1251	enriched Bifidobacterium longum subsp	1215:1251	enriched Bifidobacterium longum subsp	1215:1251	Functional changes associated with asthma protection were linked to enriched Bifidobacterium longum subsp.
36603585	11	13	theme	infant	1567:1572	arg1	microbiome					1574:1583	the infant microbiome	1563:1583	the infant microbiome	1563:1583	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	4	14	from	risk	541:544	arg1	subset					551:556	a subset	549:556	a subset of children (n = 2,521)	549:580	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	2	15	theme	gut	221:223	arg1	microbiome					225:234	the infant gut microbiome	210:234	the infant gut microbiome	210:234	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	1	16	theme	asthma	122:127	arg1	risk					129:132	the microbiota and childhood asthma risk	93:132	risk	129:132	infantis mitigates the effect of antibiotics on the microbiota and childhood asthma risk.
36603585	2	17	theme	asthma	270:275	arg1	risk					277:280	subsequent elevated pediatric asthma risk	240:280	subsequent elevated pediatric asthma risk	240:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	3	18	theme	microbiome	336:345	arg1	modulator					315:323	a primary modulator	305:323	a primary modulator of the gut microbiome	305:345	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	3	18	theme	microbiome	336:345	arg1	Breastfeeding					283:295	Breastfeeding	283:295	Breastfeeding	283:295	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	5	19	theme	fatty	746:750	arg1	composition					757:767	human milk oligosaccharide and fatty acid composition	715:767	composition	757:767	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	2	20	theme	elevated	251:258	arg1	risk					277:280	subsequent elevated pediatric asthma risk	240:280	subsequent elevated pediatric asthma risk	240:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	4	21	theme	breastfeeding	495:507	arg1	influence					482:490	the influence	478:490	the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521)	478:580	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	3	22	theme	primary	307:313	arg1	modulator					315:323	a primary modulator	305:323	a primary modulator of the gut microbiome	305:345	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	3	22	theme	primary	307:313	arg1	Breastfeeding					283:295	Breastfeeding	283:295	Breastfeeding	283:295	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	7	23	theme	re-balancing	1068:1079	arg1	"					1080:1080	widespread "re-balancing"	1056:1080	widespread "re-balancing" of taxonomic and functional components of the infant microbiome	1056:1144	This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome.
36603585	4	24	theme	antibiotic-associated	512:532	arg1	risk					541:544	antibiotic-associated asthma risk	512:544	antibiotic-associated asthma risk in a subset of children (n = 2,521)	512:580	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	5	25	theme	human	715:719	arg1	oligosaccharide					726:740	human milk oligosaccharide and fatty acid composition	715:767	oligosaccharide	726:740	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	3	26	theme	early	354:358	arg1	life					360:363	early life	354:363	early life	354:363	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	10	27	theme	human	1332:1336	arg1	oligosaccharides					1343:1358	fucosylated human milk oligosaccharides	1320:1358	fucosylated human milk oligosaccharides	1320:1358	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	1	28	theme	antibiotics	78:88	arg1	effect					68:73	the effect	64:73	the effect of antibiotics on the microbiota and childhood asthma risk	64:132	infantis mitigates the effect of antibiotics on the microbiota and childhood asthma risk.
36603585	12	29	theme	Research	1773:1780	arg1	Institutes					1752:1761	the Canadian Institutes	1739:1761	the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia	1739:1893	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	10	30	theme	paired	1363:1368	arg1	samples					1379:1385	paired maternal samples	1363:1385	paired maternal samples that were positively associated with B. infantis and these broader functional changes	1363:1471	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	12	31	theme	Canadian	1743:1750	arg1	Institutes					1752:1761	the Canadian Institutes	1739:1761	the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia	1739:1893	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	10	32	theme	oligosaccharides	1343:1358	arg1	selection					1307:1315	a selection	1305:1315	a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes	1305:1471	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	0	33	theme	subsp	38:42	arg1	enrichment					14:23	enrichment	14:23	enrichment of B. longum subsp	14:42	Breastfeeding enrichment of B. longum subsp.
36603585	5	34	theme	paired	774:779	arg1	samples					801:807	paired maternal human milk samples	774:807	paired maternal human milk samples for 561 of these infants	774:832	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	2	35	attach	linked	175:180	arg2	exposure					163:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	2	35	attach	linked	175:180	arg1	disruption					196:205	persistent disruption	185:205	persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk	185:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	5	36	theme	human	790:794	arg1	samples					801:807	paired maternal human milk samples	774:807	paired maternal human milk samples for 561 of these infants	774:832	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	7	37	theme	functional	1099:1108	arg1	components					1110:1119	taxonomic and functional components	1085:1119	taxonomic and functional components of the infant microbiome	1085:1144	This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome.
36603585	7	38	theme	components	1110:1119	arg1	"					1080:1080	widespread "re-balancing"	1056:1080	widespread "re-balancing" of taxonomic and functional components of the infant microbiome	1056:1144	This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome.
36603585	4	39	dep	METHODS	425:431	arg1	harnessed					436:444	harnessed	436:444	harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521)	436:580	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	5	40	theme	maternal	781:788	arg1	samples					801:807	paired maternal human milk samples	774:807	paired maternal human milk samples for 561 of these infants	774:832	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	11	41	contain	have	1538:1541	arg1	breastfeeding					1508:1520	breastfeeding	1508:1520	breastfeeding	1508:1520	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	11	41	contain	have	1538:1541	arg2	effects					1552:1558	opposing effects	1543:1558	opposing effects	1543:1558	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	11	41	contain	have	1538:1541	arg1	antibiotics					1526:1536	antibiotics	1526:1536	antibiotics	1526:1536	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	8	42	theme	Functional	1147:1156	arg1	changes					1158:1164	Functional changes	1147:1164	Functional changes associated with asthma protection	1147:1198	Functional changes associated with asthma protection were linked to enriched Bifidobacterium longum subsp.
36603585	6	43	theme	higher	907:912	arg1	odds					921:924	3-fold higher asthma odds	900:924	3-fold higher asthma odds	900:924	FINDINGS Children who took antibiotics without breastfeeding had 3-fold higher asthma odds, whereas there was no such association in children who received antibiotics while breastfeeding.
36603585	12	44	theme	Genome	1852:1857	arg1	Canada					1859:1864	Genome Canada	1852:1864	Genome Canada	1852:1864	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	5	45	theme	children	644:651	arg1	subset					628:633	a subset	626:633	a subset of these children (n = 1,338) using shotgun metagenomic sequencing	626:700	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	10	46	theme	functional	1454:1463	arg1	changes					1465:1471	these broader functional changes	1440:1471	these broader functional changes	1440:1471	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	1	47	from	effect	68:73	arg1	risk					129:132	the microbiota and childhood asthma risk	93:132	risk	129:132	infantis mitigates the effect of antibiotics on the microbiota and childhood asthma risk.
36603585	1	47	from	effect	68:73	arg1	microbiota					97:106	the microbiota and childhood asthma risk	93:132	microbiota	97:106	infantis mitigates the effect of antibiotics on the microbiota and childhood asthma risk.
36603585	2	48	theme	antibiotic	152:161	arg1	exposure					163:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	11	49	theme	B. infantis	1622:1632	arg1	enrichment					1608:1617	breastfeeding enrichment	1594:1617	breastfeeding enrichment of B. infantis	1594:1632	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	8	50	theme	asthma	1182:1187	arg1	protection					1189:1198	asthma protection	1182:1198	asthma protection	1182:1198	Functional changes associated with asthma protection were linked to enriched Bifidobacterium longum subsp.
36603585	12	51	theme	Centres	1829:1835	arg1	Network					1818:1824	Environment Network	1806:1824	Environment Network	1806:1824	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	12	51	theme	Centres	1829:1835	arg1	Canada					1859:1864	Genome Canada	1852:1864	Genome Canada	1852:1864	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	12	51	theme	Centres	1829:1835	arg1	Allergy					1787:1793	Allergy	1787:1793	Allergy	1787:1793	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	12	51	theme	Centres	1829:1835	arg1	Genome					1871:1876	Genome	1871:1876	Genome	1871:1876	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	12	51	theme	Centres	1829:1835	arg1	Genes					1796:1800	Genes	1796:1800	Genes	1796:1800	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	12	51	theme	Centres	1829:1835	arg1	Research					1773:1780	Health Research	1766:1780	Health Research	1766:1780	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	2	52	theme	BACKGROUND	135:144	arg1	exposure					163:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure	135:170	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	11	53	theme	asthma	1683:1688	arg1	risk					1690:1693	reduced antibiotic-associated asthma risk	1653:1693	reduced antibiotic-associated asthma risk	1653:1693	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	12	54	theme	Excellence	1840:1849	arg1	Centres					1829:1835	Centres	1829:1835	Centres of Excellence	1829:1849	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	5	55	theme	shotgun	671:677	arg1	sequencing					691:700	shotgun metagenomic sequencing	671:700	shotgun metagenomic sequencing	671:700	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	11	56	theme	reduced	1653:1659	arg1	risk					1690:1693	reduced antibiotic-associated asthma risk	1653:1693	reduced antibiotic-associated asthma risk	1653:1693	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	6	57	theme	such	948:951	arg1	association					953:963	no such association	945:963	no such association	945:963	FINDINGS Children who took antibiotics without breastfeeding had 3-fold higher asthma odds, whereas there was no such association in children who received antibiotics while breastfeeding.
36603585	4	58	theme	CHILD	450:454	arg1	cohort					456:461	the CHILD cohort	446:461	the CHILD cohort	446:461	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	7	59	theme	widespread	1056:1065	arg1	"					1080:1080	widespread "re-balancing"	1056:1080	widespread "re-balancing" of taxonomic and functional components of the infant microbiome	1056:1144	This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome.
36603585	2	60	theme	subsequent	240:249	arg1	risk					277:280	subsequent elevated pediatric asthma risk	240:280	subsequent elevated pediatric asthma risk	240:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	5	61	theme	infants	826:832	arg1	561					813:815	561	813:815	561 of these infants	813:832	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	8	62	attach	linked	1205:1210	arg1	subsp					1247:1251	enriched Bifidobacterium longum subsp	1215:1251	enriched Bifidobacterium longum subsp	1215:1251	Functional changes associated with asthma protection were linked to enriched Bifidobacterium longum subsp.
36603585	8	62	attach	linked	1205:1210	arg2	changes					1158:1164	Functional changes	1147:1164	Functional changes associated with asthma protection	1147:1198	Functional changes associated with asthma protection were linked to enriched Bifidobacterium longum subsp.
36603585	6	63	contain	had	896:898	arg2	odds					921:924	3-fold higher asthma odds	900:924	3-fold higher asthma odds	900:924	FINDINGS Children who took antibiotics without breastfeeding had 3-fold higher asthma odds, whereas there was no such association in children who received antibiotics while breastfeeding.
36603585	6	63	contain	had	896:898	arg1	Children					844:851	FINDINGS Children	835:851	FINDINGS Children who took antibiotics without breastfeeding	835:894	FINDINGS Children who took antibiotics without breastfeeding had 3-fold higher asthma odds, whereas there was no such association in children who received antibiotics while breastfeeding.
36603585	2	64	theme	microbiome	225:234	arg1	disruption					196:205	persistent disruption	185:205	persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk	185:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	1	65	theme	childhood	112:120	arg1	risk					129:132	the microbiota and childhood asthma risk	93:132	risk	129:132	infantis mitigates the effect of antibiotics on the microbiota and childhood asthma risk.
36603585	11	66	dep	CONCLUSIONS	1474:1484	arg1	suggest					1495:1501	suggest	1495:1501	suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk	1495:1693	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	10	67	theme	Network	1277:1283	arg1	analysis					1285:1292	Network analysis	1277:1292	Network analysis	1277:1292	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	2	68	theme	risk	277:280	arg1	disruption					196:205	persistent disruption	185:205	persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk	185:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	10	69	gly	fucosylated	1320:1330	arg1	oligosaccharides					1343:1358	fucosylated human milk oligosaccharides	1320:1358	fucosylated human milk oligosaccharides	1320:1358	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	2	70	theme	pediatric	260:268	arg1	risk					277:280	subsequent elevated pediatric asthma risk	240:280	subsequent elevated pediatric asthma risk	240:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	12	71	theme	Environment	1806:1816	arg1	Network					1818:1824	Environment Network	1806:1824	Environment Network	1806:1824	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	11	72	theme	breastfeeding	1594:1606	arg1	enrichment					1608:1617	breastfeeding enrichment	1594:1617	breastfeeding enrichment of B. infantis	1594:1632	CONCLUSIONS Our data suggest that breastfeeding and antibiotics have opposing effects on the infant microbiome and that breastfeeding enrichment of B. infantis is associated with reduced antibiotic-associated asthma risk.
36603585	5	73	theme	acid	752:755	arg1	composition					757:767	human milk oligosaccharide and fatty acid composition	715:767	composition	757:767	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	3	74	theme	gut	332:334	arg1	microbiome					336:345	the gut microbiome	328:345	the gut microbiome	328:345	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	4	75	theme	asthma	534:539	arg1	risk					541:544	antibiotic-associated asthma risk	512:544	antibiotic-associated asthma risk in a subset of children (n = 2,521)	512:580	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	12	76	theme	Allergy	1787:1793	arg1	Institutes					1752:1761	the Canadian Institutes	1739:1761	the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia	1739:1893	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	12	77	theme	Genome	1871:1876	arg1	Institutes					1752:1761	the Canadian Institutes	1739:1761	the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia	1739:1893	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	10	78	theme	milk	1338:1341	arg1	oligosaccharides					1343:1358	fucosylated human milk oligosaccharides	1320:1358	fucosylated human milk oligosaccharides	1320:1358	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	5	79	theme	milk	721:724	arg1	oligosaccharide					726:740	human milk oligosaccharide and fatty acid composition	715:767	oligosaccharide	726:740	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	3	80	from	effect	374:379	arg1	development					391:401	asthma development	384:401	asthma development	384:401	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	5	81	theme	infant	604:609	arg1	microbiomes					611:621	the infant microbiomes	600:621	the infant microbiomes	600:621	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	10	82	theme	fucosylated	1320:1330	arg1	oligosaccharides					1343:1358	fucosylated human milk oligosaccharides	1320:1358	fucosylated human milk oligosaccharides	1320:1358	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	5	83	theme	milk	796:799	arg1	samples					801:807	paired maternal human milk samples	774:807	paired maternal human milk samples for 561 of these infants	774:832	We then profiled the infant microbiomes in a subset of these children (n = 1,338) using shotgun metagenomic sequencing and compared human milk oligosaccharide and fatty acid composition from paired maternal human milk samples for 561 of these infants.
36603585	12	84	theme	Health	1766:1771	arg1	Research					1773:1780	Health Research	1766:1780	Health Research	1766:1780	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
36603585	3	85	theme	asthma	384:389	arg1	development					391:401	asthma development	384:401	asthma development	384:401	Breastfeeding acts as a primary modulator of the gut microbiome during early life, but its effect on asthma development has remained unclear.
36603585	4	86	theme	children	561:568	arg1	subset					551:556	a subset	549:556	a subset of children (n = 2,521)	549:580	METHODS We harnessed the CHILD cohort to interrogate the influence of breastfeeding on antibiotic-associated asthma risk in a subset of children (n = 2,521).
36603585	10	87	theme	maternal	1370:1377	arg1	samples					1379:1385	paired maternal samples	1363:1385	paired maternal samples that were positively associated with B. infantis and these broader functional changes	1363:1471	Network analysis identified a selection of fucosylated human milk oligosaccharides in paired maternal samples that were positively associated with B. infantis and these broader functional changes.
36603585	0	88	theme	B. longum	28:36	arg1	subsp					38:42	B. longum subsp	28:42	B. longum subsp	28:42	Breastfeeding enrichment of B. longum subsp.
36603585	2	89	theme	persistent	185:194	arg1	disruption					196:205	persistent disruption	185:205	persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk	185:280	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	7	90	theme	microbiome	1135:1144	arg1	components					1110:1119	taxonomic and functional components	1085:1119	taxonomic and functional components of the infant microbiome	1085:1144	This benefit was associated with widespread "re-balancing" of taxonomic and functional components of the infant microbiome.
36603585	6	91	theme	FINDINGS	835:842	arg1	Children					844:851	FINDINGS Children	835:851	FINDINGS Children who took antibiotics without breastfeeding	835:894	FINDINGS Children who took antibiotics without breastfeeding had 3-fold higher asthma odds, whereas there was no such association in children who received antibiotics while breastfeeding.
36603585	2	92	theme	infant	214:219	arg1	microbiome					225:234	the infant gut microbiome	210:234	the infant gut microbiome	210:234	BACKGROUND Early antibiotic exposure is linked to persistent disruption of the infant gut microbiome and subsequent elevated pediatric asthma risk.
36603585	12	93	dep	Research	1773:1780	arg1	Columbia					1886:1893	Columbia	1886:1893	Columbia	1886:1893	FUNDING This work was supported in part by the Canadian Institutes of Health Research; the Allergy, Genes and Environment Network of Centres of Excellence; Genome Canada; and Genome British Columbia.
35847597	5	0	theme	gas	573:575	arg1	GC-MS					611:615	GC-MS	611:615	GC-MS	611:615	The EO compounds were analyzed by gas chromatography-mass spectrometry (GC-MS).
35847597	5	0	theme	gas	573:575	arg1	spectrometry					597:608	gas chromatography-mass spectrometry	573:608	gas chromatography-mass spectrometry (GC-MS)	573:616	The EO compounds were analyzed by gas chromatography-mass spectrometry (GC-MS).
35847597	2	1	theme	response	311:318	arg1	methodology					328:338	response surface methodology	311:338	response surface methodology	311:338	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	12	2	theme	<	1649:1649	arg1	P					1647:1647	P < 0.01	1647:1654	P < 0.01	1647:1654	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	12	2	theme	<	1649:1649	arg1	group					1640:1644	the DSS group	1632:1644	the DSS group (P < 0.01)	1632:1655	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	11	3	theme	normal	1412:1417	arg1	group					1419:1423	normal group	1412:1423	normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm)	1412:1546	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	14	4	theme	treatment	1798:1806	arg1	groups					1808:1813	the EO treatment groups	1791:1813	the EO treatment groups	1791:1813	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	6	5	theme	peak	785:788	arg1	area					790:793	the total peak area	775:793	the total peak area	775:793	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	12	6	theme	DSS	1636:1638	arg1	P					1647:1647	P < 0.01	1647:1654	P < 0.01	1647:1654	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	12	6	theme	DSS	1636:1638	arg1	group					1640:1644	the DSS group	1632:1644	the DSS group (P < 0.01)	1632:1655	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	15	7	theme	P	1984:1984	arg1	<					1986:1986	P < 0.05	1984:1991	P < 0.05	1984:1991	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	11	8	from	length	1253:1258	arg1	group					1275:1279	DSS group	1271:1279	DSS group	1271:1279	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	15	9	theme	EO	1887:1888	arg1	group					1900:1904	EO treatment group	1887:1904	EO treatment group	1887:1904	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	7	10	theme	reducing	948:955	arg1	power					957:961	reducing power	948:961	reducing power	948:961	The EO showed good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power.
35847597	16	11	theme	colitis	2076:2082	arg1	symptoms					2064:2071	the symptoms	2060:2071	the symptoms of colitis	2060:2082	This study shows that EO can effectively improve the symptoms of colitis.
35847597	11	12	theme	mice	1323:1326	arg1	length					1313:1318	the colon length	1303:1318	the colon length of mice in EO treatment group	1303:1348	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	2	13	theme	steam	217:221	arg1	distillation					223:234	steam distillation	217:234	steam distillation	217:234	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	1	14	theme	Sodium	122:127	arg1	Colitis					145:151	Dextran Sodium Sulfate-Induced Colitis	114:151	Dextran Sodium Sulfate-Induced Colitis in Mice	114:159	Leaves on Dextran Sodium Sulfate-Induced Colitis in Mice.
35847597	0	15	from	Chimonanthus	78:89	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	0	15	from	Chimonanthus	78:89	arg1	Composition					21:31	Chemical Composition	12:31	Chemical Composition	12:31	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	0	15	from	Chimonanthus	78:89	arg1	Effect					49:54	Protective Effect	38:54	Protective Effect	38:54	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	2	16	dep	Chimonanthus	241:252	arg1	nitens					254:259	nitens	254:259	nitens	254:259	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	15	17	theme	L-EO	1946:1949	arg1	group					1935:1939	model group	1929:1939	model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05)	1929:2008	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	15	17	theme	L-EO	1946:1949	arg1	group					1951:1955	the L-EO group	1942:1955	the L-EO group was not significant	1942:1975	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	0	18	theme	Oil	69:71	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	0	18	theme	Oil	69:71	arg1	Composition					21:31	Chemical Composition	12:31	Chemical Composition	12:31	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	0	18	theme	Oil	69:71	arg1	Effect					49:54	Protective Effect	38:54	Protective Effect	38:54	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	15	19	theme	model	1929:1933	arg1	group					1935:1939	model group	1929:1939	model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05)	1929:2008	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	15	19	theme	model	1929:1933	arg1	group					1951:1955	the L-EO group	1942:1955	the L-EO group was not significant	1942:1975	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	12	20	theme	SASP	1582:1585	arg1	groups					1587:1592	the L-EO and SASP groups	1569:1592	the L-EO and SASP groups	1569:1592	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	15	21	theme	P	2000:2000	arg1	<					2002:2002	P < 0.05	2000:2007	P < 0.05	2000:2007	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	8	22	from	effect	1006:1011	arg1	UC					1042:1043	UC	1042:1043	UC	1042:1043	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	8	22	from	effect	1006:1011	arg1	colitis					1033:1039	ulcerative colitis	1022:1039	ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice	1022:1092	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	11	23	theme	EO	1331:1332	arg1	group					1344:1348	EO treatment group	1331:1348	EO treatment group	1331:1348	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	14	24	theme	P	1869:1869	arg1	<					1871:1871	P < 0.05	1869:1876	P < 0.05	1869:1876	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	3	25	theme	process	353:359	arg1	conditions					361:370	The optimum process conditions	341:370	The optimum process conditions	341:370	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	26	theme	extraction	388:397	arg1	time					399:402	extraction time	388:402	extraction time of 4.57 h	388:412	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	11	27	dep	NOR	1426:1428	arg1	0.39 cm					1439:1445	8.17 ± 0.39 cm	1432:1445	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	12	28	theme	L-EO	1573:1576	arg1	groups					1587:1592	the L-EO and SASP groups	1569:1592	the L-EO and SASP groups	1569:1592	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	7	29	theme	good	810:813	arg1	activity					827:834	good antioxidant activity	810:834	good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power	810:961	The EO showed good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power.
35847597	11	30	from	length	1313:1318	arg1	group					1344:1348	EO treatment group	1331:1348	EO treatment group	1331:1348	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	10	31	from	reduction	1206:1214	arg1	group					1236:1240	the EO group	1229:1240	the EO group	1229:1240	Histological examination also observed a significant reduction in damage in the EO group.
35847597	10	31	from	reduction	1206:1214	arg1	damage					1219:1224	damage	1219:1224	damage	1219:1224	Histological examination also observed a significant reduction in damage in the EO group.
35847597	15	32	dep	group	1951:1955	arg1	M-EO					1978:1981	M-EO	1978:1981	M-EO	1978:1981	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	15	32	dep	group	1951:1955	arg1	<					1986:1986	P < 0.05	1984:1991	P < 0.05	1984:1991	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	15	32	dep	group	1951:1955	arg1	H-EO					1994:1997	H-EO	1994:1997	H-EO	1994:1997	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	15	32	dep	group	1951:1955	arg1	<					2002:2002	P < 0.05	2000:2007	P < 0.05	2000:2007	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	0	33	theme	Chemical	12:19	arg1	Composition					21:31	Chemical Composition	12:31	Chemical Composition	12:31	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	10	34	theme	Histological	1153:1164	arg1	examination					1166:1176	Histological examination	1153:1176	Histological examination	1153:1176	Histological examination also observed a significant reduction in damage in the EO group.
35847597	11	35	dep	0.39 cm	1439:1445	arg1	0.58 cm					1511:1517	5.98 ± 0.58 cm	1504:1517	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	EO					1474:1475	EO	1474:1475	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	M					1495:1495	M	1495:1495	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	0.52 cm					1539:1545	6.1 ± 0.52 cm	1533:1545	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	EO					1499:1500	EO	1499:1500	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	and H					1520:1524	and H	1520:1524	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	0.93 cm					1461:1467	5.57 ± 0.93 cm	1454:1467	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	EO					1528:1529	EO	1528:1529	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	0.78 cm					1486:1492	6.47 ± 0.78 cm	1479:1492	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	DSS					1448:1450	DSS	1448:1450	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	35	dep	0.39 cm	1439:1445	arg1	L					1470:1470	L	1470:1470	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	12	36	theme	GSH	1553:1555	arg1	higher					1612:1617	higher	1612:1617	higher	1612:1617	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	12	36	theme	GSH	1553:1555	arg1	activity					1557:1564	The GSH activity	1549:1564	The GSH activity in the L-EO and SASP groups	1549:1592	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	11	37	theme	±	1459:1459	arg1	0.93 cm					1461:1467	5.57 ± 0.93 cm	1454:1467	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	13	38	theme	SOD	1658:1660	arg1	activity					1662:1669	SOD activity	1658:1669	SOD activity in L-EO and M-EO groups	1658:1693	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	0	39	theme	Protective	38:47	arg1	Effect					49:54	Protective Effect	38:54	Protective Effect	38:54	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	2	40	theme	essential	181:189	arg1	EO					196:197	EO	196:197	EO	196:197	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	2	40	theme	essential	181:189	arg1	oil					191:193	the essential oil	177:193	the essential oil (EO)	177:198	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	3	41	theme	1.33 h	431:436	arg1	time					423:426	soaking time	415:426	soaking time of 1.33 h	415:436	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	41	theme	1.33 h	431:436	arg1	time					399:402	extraction time	388:402	extraction time of 4.57 h	388:412	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	41	theme	1.33 h	431:436	arg1	ratio					456:460	solid-liquid ratio	443:460	solid-liquid ratio of 1 	443:466	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	42	theme	1 	465:466	arg1	time					423:426	soaking time	415:426	soaking time of 1.33 h	415:436	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	42	theme	1 	465:466	arg1	time					399:402	extraction time	388:402	extraction time of 4.57 h	388:412	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	42	theme	1 	465:466	arg1	ratio					456:460	solid-liquid ratio	443:460	solid-liquid ratio of 1 	443:466	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	8	43	theme	EO	1016:1017	arg1	effect					1006:1011	the protective effect	991:1011	the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice	991:1092	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	8	44	from	sulfate	1072:1078	arg1	mice					1089:1092	mice	1089:1092	mice	1089:1092	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	8	45	theme	dextran	1057:1063	arg1	DSS					1081:1083	DSS	1081:1083	DSS	1081:1083	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	8	45	theme	dextran	1057:1063	arg1	sulfate					1072:1078	dextran sodium sulfate	1057:1078	dextran sodium sulfate (DSS) in mice	1057:1092	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	11	46	theme	±	1437:1437	arg1	0.39 cm					1439:1445	8.17 ± 0.39 cm	1432:1445	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	6	47	theme	chemical	633:640	arg1	components					642:651	52 chemical components	630:651	52 chemical components	630:651	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	2	48	theme	surface	320:326	arg1	methodology					328:338	response surface methodology	311:338	response surface methodology	311:338	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	13	49	theme	<	1761:1761	arg1	group					1752:1756	DSS treatment group	1738:1756	DSS treatment group (P < 0.01)	1738:1767	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	13	49	theme	<	1761:1761	arg1	P					1759:1759	P < 0.01	1759:1766	P < 0.01	1759:1766	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	8	50	theme	ulcerative	1022:1031	arg1	UC					1042:1043	UC	1042:1043	UC	1042:1043	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	8	50	theme	ulcerative	1022:1031	arg1	colitis					1033:1039	ulcerative colitis	1022:1039	ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice	1022:1092	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	11	51	theme	mice	1263:1266	arg1	shortest					1289:1296	shortest	1289:1296	shortest	1289:1296	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	51	theme	mice	1263:1266	arg1	length					1253:1258	The colon length	1243:1258	The colon length of mice in DSS group	1243:1279	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	5	52	theme	chromatography-mass	577:595	arg1	GC-MS					611:615	GC-MS	611:615	GC-MS	611:615	The EO compounds were analyzed by gas chromatography-mass spectrometry (GC-MS).
35847597	5	52	theme	chromatography-mass	577:595	arg1	spectrometry					597:608	gas chromatography-mass spectrometry	573:608	gas chromatography-mass spectrometry (GC-MS)	573:616	The EO compounds were analyzed by gas chromatography-mass spectrometry (GC-MS).
35847597	6	53	theme	-furan	726:731	arg1	content					684:690	the content	680:690	the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan	680:731	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	6	53	theme	-furan	726:731	arg1	highest					741:747	highest	741:747	highest	741:747	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	6	54	theme	area	790:793	arg1	area					790:793	the total peak area	775:793	the total peak area	775:793	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	6	54	theme	area	790:793	arg1	%					770:770	21.43%	765:770	21.43% of the total peak area	765:793	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	4	55	theme	EO	524:525	arg1	%					536:536	1.5624%	530:536	1.5624%	530:536	Under these conditions, the theoretical yield of EO is 1.5624%.
35847597	4	55	theme	EO	524:525	arg1	yield					515:519	the theoretical yield	499:519	the theoretical yield of EO	499:525	Under these conditions, the theoretical yield of EO is 1.5624%.
35847597	14	56	theme	EO	1795:1796	arg1	groups					1808:1813	the EO treatment groups	1791:1813	the EO treatment groups	1791:1813	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	5	57	theme	EO	543:544	arg1	compounds					546:554	The EO compounds	539:554	The EO compounds	539:554	The EO compounds were analyzed by gas chromatography-mass spectrometry (GC-MS).
35847597	2	58	theme	Chimonanthus	241:252	arg1	Oliv					261:264	Chimonanthus nitens Oliv	241:264	Chimonanthus nitens Oliv	241:264	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	6	59	theme	total	779:783	arg1	area					790:793	the total peak area	775:793	the total peak area	775:793	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	14	60	dep	H-EO	1841:1844	arg1	SASP					1847:1850	SASP	1847:1850	SASP: P < 0.01; M-EO	1847:1866	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	14	60	dep	H-EO	1841:1844	arg1	<					1871:1871	P < 0.05	1869:1876	P < 0.05	1869:1876	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	6	61	theme	3-	695:696	arg1	-furan					726:731	3-(4,8-dimethylnona-3,7-dienyl)-furan	695:731	3-(4,8-dimethylnona-3,7-dienyl)-furan	695:731	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
35847597	2	62	theme	extraction	275:284	arg1	process					286:292	the extraction process	271:292	the extraction process	271:292	In this study, the essential oil (EO) was extracted by steam distillation from Chimonanthus nitens Oliv, and the extraction process was optimized by response surface methodology.
35847597	12	63	from	activity	1557:1564	arg1	groups					1587:1592	the L-EO and SASP groups	1569:1592	the L-EO and SASP groups	1569:1592	The GSH activity in the L-EO and SASP groups was significantly higher than that in the DSS group (P < 0.01).
35847597	14	64	dep	SASP	1847:1850	arg1	M-EO					1863:1866	M-EO	1863:1866	SASP: P < 0.01; M-EO	1847:1866	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	14	64	dep	SASP	1847:1850	arg1	P					1853:1853	P < 0.01	1853:1860	SASP: P < 0.01; M-EO	1847:1866	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	11	65	theme	model	1374:1378	arg1	group					1380:1384	model group	1374:1384	model group	1374:1384	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	13	66	from	activity	1662:1669	arg1	M-EO					1683:1686	M-EO	1683:1686	M-EO	1683:1686	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	13	66	from	activity	1662:1669	arg1	L-EO					1674:1677	L-EO	1674:1677	L-EO	1674:1677	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	8	67	theme	sodium	1065:1070	arg1	DSS					1081:1083	DSS	1081:1083	DSS	1081:1083	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	8	67	theme	sodium	1065:1070	arg1	sulfate					1072:1078	dextran sodium sulfate	1057:1078	dextran sodium sulfate (DSS) in mice	1057:1092	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	11	68	dep	group	1419:1423	arg1	NOR					1426:1428	NOR	1426:1428	NOR	1426:1428	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	69	theme	±	1509:1509	arg1	0.58 cm					1511:1517	5.98 ± 0.58 cm	1504:1517	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	15	70	theme	treatment	1890:1898	arg1	group					1900:1904	EO treatment group	1887:1904	EO treatment group	1887:1904	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	9	71	theme	weight	1118:1123	arg1	loss					1125:1128	weight loss	1118:1128	weight loss	1118:1128	EO effectively delayed weight loss and reduced DAI score.
35847597	1	72	theme	Dextran	114:120	arg1	Colitis					145:151	Dextran Sodium Sulfate-Induced Colitis	114:151	Dextran Sodium Sulfate-Induced Colitis in Mice	114:159	Leaves on Dextran Sodium Sulfate-Induced Colitis in Mice.
35847597	11	73	theme	colon	1307:1311	arg1	length					1313:1318	the colon length	1303:1318	the colon length of mice in EO treatment group	1303:1348	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	0	74	theme	Essential	59:67	arg1	Oil					69:71	Essential Oil	59:71	Essential Oil	59:71	Extraction, Chemical Composition, and Protective Effect of Essential Oil from Chimonanthus nitens Oliv.
35847597	13	75	theme	DSS	1738:1740	arg1	group					1752:1756	DSS treatment group	1738:1756	DSS treatment group (P < 0.01)	1738:1767	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	13	75	theme	DSS	1738:1740	arg1	P					1759:1759	P < 0.01	1759:1766	P < 0.01	1759:1766	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	7	76	theme	3-ethylbenzthiazoline-6-sulfonic	897:928	arg1	2,2'-azino-bis					882:895	2,2'-azino-bis	882:895	2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS)	882:941	The EO showed good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power.
35847597	7	76	theme	3-ethylbenzthiazoline-6-sulfonic	897:928	arg1	acid					930:933	3-ethylbenzthiazoline-6-sulfonic acid	897:933	3-ethylbenzthiazoline-6-sulfonic acid	897:933	The EO showed good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power.
35847597	1	77	theme	Sulfate-Induced	129:143	arg1	Colitis					145:151	Dextran Sodium Sulfate-Induced Colitis	114:151	Dextran Sodium Sulfate-Induced Colitis in Mice	114:159	Leaves on Dextran Sodium Sulfate-Induced Colitis in Mice.
35847597	11	78	theme	treatment	1334:1342	arg1	group					1344:1348	EO treatment group	1331:1348	EO treatment group	1331:1348	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	79	theme	±	1484:1484	arg1	0.78 cm					1486:1492	6.47 ± 0.78 cm	1479:1492	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	9	80	theme	DAI	1142:1144	arg1	score					1146:1150	DAI score	1142:1150	DAI score	1142:1150	EO effectively delayed weight loss and reduced DAI score.
35847597	13	81	theme	treatment	1742:1750	arg1	group					1752:1756	DSS treatment group	1738:1756	DSS treatment group (P < 0.01)	1738:1767	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	13	81	theme	treatment	1742:1750	arg1	P					1759:1759	P < 0.01	1759:1766	P < 0.01	1759:1766	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	1	82	from	Colitis	145:151	arg1	Mice					156:159	Mice	156:159	Mice	156:159	Leaves on Dextran Sodium Sulfate-Induced Colitis in Mice.
35847597	11	83	theme	DSS	1271:1273	arg1	group					1275:1279	DSS group	1271:1279	DSS group	1271:1279	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	3	84	theme	4.57 h	407:412	arg1	time					423:426	soaking time	415:426	soaking time of 1.33 h	415:436	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	84	theme	4.57 h	407:412	arg1	time					399:402	extraction time	388:402	extraction time of 4.57 h	388:412	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	3	84	theme	4.57 h	407:412	arg1	ratio					456:460	solid-liquid ratio	443:460	solid-liquid ratio of 1 	443:466	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	7	85	theme	antioxidant	815:825	arg1	activity					827:834	good antioxidant activity	810:834	good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power	810:961	The EO showed good antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and reducing power.
35847597	3	86	theme	optimum	345:351	arg1	process					353:359	The optimum process	341:359	The optimum process conditions	341:370	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	4	87	theme	theoretical	503:513	arg1	%					536:536	1.5624%	530:536	1.5624%	530:536	Under these conditions, the theoretical yield of EO is 1.5624%.
35847597	4	87	theme	theoretical	503:513	arg1	yield					515:519	the theoretical yield	499:519	the theoretical yield of EO	499:525	Under these conditions, the theoretical yield of EO is 1.5624%.
35847597	3	88	theme	soaking	415:421	arg1	time					423:426	soaking time	415:426	soaking time of 1.33 h	415:436	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	15	89	theme	group	1900:1904	arg1	MPO					1880:1882	MPO	1880:1882	MPO of EO treatment group	1880:1904	MPO of EO treatment group was lower than that of model group (the L-EO group was not significant, M-EO: P < 0.05, H-EO: P < 0.05).
35847597	8	90	theme	protective	995:1004	arg1	effect					1006:1011	the protective effect	991:1011	the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice	991:1092	In this study, we observed the protective effect of EO on ulcerative colitis (UC) induced by dextran sodium sulfate (DSS) in mice.
35847597	14	91	theme	SASP	1823:1826	arg1	group					1828:1832	the SASP group	1819:1832	the SASP group	1819:1832	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	14	92	dep	decreased	1778:1786	arg1	H-EO					1841:1844	H-EO	1841:1844	H-EO	1841:1844	MDA was decreased in the EO treatment groups and the SASP group (L-EO, H-EO, SASP: P < 0.01; M-EO: P < 0.05).
35847597	13	93	dep	L-EO	1674:1677	arg1	groups					1688:1693	groups	1688:1693	groups	1688:1693	SOD activity in L-EO and M-EO groups was also significantly higher than that in DSS treatment group (P < 0.01).
35847597	10	94	theme	significant	1194:1204	arg1	reduction					1206:1214	a significant reduction	1192:1214	a significant reduction in damage in the EO group	1192:1240	Histological examination also observed a significant reduction in damage in the EO group.
35847597	3	95	theme	solid-liquid	443:454	arg1	ratio					456:460	solid-liquid ratio	443:460	solid-liquid ratio of 1 	443:466	The optimum process conditions are as follows: extraction time of 4.57 h, soaking time of 1.33 h, and solid-liquid ratio of 1 : 21.4.
35847597	10	96	theme	EO	1233:1234	arg1	group					1236:1240	the EO group	1229:1240	the EO group	1229:1240	Histological examination also observed a significant reduction in damage in the EO group.
35847597	11	97	theme	±	1537:1537	arg1	0.52 cm					1539:1545	6.1 ± 0.52 cm	1533:1545	8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm	1432:1545	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	98	dep	that	1366:1369	arg1	longer					1354:1359	longer	1354:1359	longer	1354:1359	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	99	theme	colon	1247:1251	arg1	shortest					1289:1296	shortest	1289:1296	shortest	1289:1296	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	11	99	theme	colon	1247:1251	arg1	length					1253:1258	The colon length	1243:1258	The colon length of mice in DSS group	1243:1279	The colon length of mice in DSS group was the shortest, and the colon length of mice in EO treatment group was longer than that in model group, but shorter than that in normal group (NOR : 8.17 ± 0.39 cm; DSS : 5.57 ± 0.93 cm; L - EO : 6.47 ± 0.78 cm; M - EO : 5.98 ± 0.58 cm; and H - EO : 6.1 ± 0.52 cm).
35847597	6	100	theme	components	642:651	arg1	total					621:625	A total	619:625	A total of 52 chemical components	619:651	A total of 52 chemical components were detected, among which the content of 3-(4,8-dimethylnona-3,7-dienyl)-furan was the highest, accounting for 21.43% of the total peak area.
36234886	0	0	theme	Glycemic	65:72	arg1	Index					74:78	Glycemic Index	65:78	Glycemic Index	65:78	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	2	1	from	addition	376:383	arg1	level					412:416	the level	408:416	the level of 5, 10, 15, 20, and 25%	408:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	4	2	theme	starch	694:699	arg1	content					676:682	the digestible carbohydrate content	648:682	the digestible carbohydrate content	648:682	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	2	theme	starch	694:699	arg1	HI					719:720	HI	719:720	HI	719:720	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	2	theme	starch	694:699	arg1	index					712:716	in vitro starch hydrolysis index	685:716	in vitro starch hydrolysis index (HI)	685:721	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	8	3	theme	≤	1183:1183	arg1	p					1181:1181	p ≤ 0.05	1181:1188	p ≤ 0.05	1181:1188	The functional addition significantly (p ≤ 0.05) increased the loss of dry matter and influenced the cooking time of pasta.
36234886	7	4	theme	cake	1109:1112	arg1	addition					1085:1092	the 25% addition	1077:1092	the 25% addition of black cumin cake	1077:1112	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	8	5	theme	dry	1213:1215	arg1	matter					1217:1222	dry matter	1213:1222	dry matter	1213:1222	The functional addition significantly (p ≤ 0.05) increased the loss of dry matter and influenced the cooking time of pasta.
36234886	7	6	theme	iron	1019:1022	arg1	content					1024:1030	the iron content	1015:1030	the iron content	1015:1030	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	0	7	from	Effect	0:5	arg1	Quality					115:121	Cooking Quality	107:121	Cooking Quality	107:121	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	0	7	from	Effect	0:5	arg1	Composition					52:62	Chemical Composition	43:62	Chemical Composition	43:62	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	0	7	from	Effect	0:5	arg1	Index					74:78	Glycemic Index	65:78	Glycemic Index	65:78	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	0	7	from	Effect	0:5	arg1	Activity					93:100	Antioxidant Activity	81:100	Antioxidant Activity	81:100	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	4	8	theme	digestible	652:661	arg1	content					676:682	the digestible carbohydrate content	648:682	the digestible carbohydrate content	648:682	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	8	theme	digestible	652:661	arg1	index					712:716	in vitro starch hydrolysis index	685:716	in vitro starch hydrolysis index (HI)	685:721	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	6	9	theme	higher	957:962	arg1	activity					976:983	higher antioxidant activity	957:983	higher antioxidant activity against DPPH	957:996	The content of polyphenols, including flavonoids, in the cake-enriched pasta increased significantly (p ≤ 0.05), which resulted in higher antioxidant activity against DPPH.
36234886	6	10	theme	≤	930:930	arg1	p					928:928	p ≤ 0.05	928:935	p ≤ 0.05	928:935	The content of polyphenols, including flavonoids, in the cake-enriched pasta increased significantly (p ≤ 0.05), which resulted in higher antioxidant activity against DPPH.
36234886	3	11	theme	ash	565:567	arg1	content					540:546	the content	536:546	the content of protein, fat, ash, and fiber	536:578	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	4	12	theme	hydrolysis	701:710	arg1	content					676:682	the digestible carbohydrate content	648:682	the digestible carbohydrate content	648:682	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	12	theme	hydrolysis	701:710	arg1	HI					719:720	HI	719:720	HI	719:720	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	12	theme	hydrolysis	701:710	arg1	index					712:716	in vitro starch hydrolysis index	685:716	in vitro starch hydrolysis index (HI)	685:721	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	7	13	theme	2.5	1041:1043	arg1	increase					1003:1010	The increase	999:1010	The increase in the iron content	999:1030	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	7	13	theme	2.5	1041:1043	arg1	times					1045:1049	times	1045:1049	times	1045:1049	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	8	14	theme	cooking	1243:1249	arg1	time					1251:1254	the cooking time	1239:1254	the cooking time of pasta	1239:1263	The functional addition significantly (p ≤ 0.05) increased the loss of dry matter and influenced the cooking time of pasta.
36234886	7	15	theme	control	1126:1132	arg1	sample					1134:1139	the control sample	1122:1139	the control sample	1122:1139	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	7	16	dep	times	1045:1049	arg1	higher					1051:1056	higher	1051:1056	higher	1051:1056	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	7	17	theme	%	1083:1083	arg1	addition					1085:1092	the 25% addition	1077:1092	the 25% addition of black cumin cake	1077:1112	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	0	18	theme	Antioxidant	81:91	arg1	Activity					93:100	Antioxidant Activity	81:100	Antioxidant Activity	81:100	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	2	19	from	level	412:416	arg1	addition					376:383	the addition	372:383	the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%	372:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	2	19	from	level	412:416	arg1	cake					400:403	black cumin cake	388:403	black cumin cake at the level of 5, 10, 15, 20, and 25%	388:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	4	20	theme	carbohydrate	663:674	arg1	content					676:682	the digestible carbohydrate content	648:682	the digestible carbohydrate content	648:682	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	20	theme	carbohydrate	663:674	arg1	index					712:716	in vitro starch hydrolysis index	685:716	in vitro starch hydrolysis index (HI)	685:721	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	6	21	from	content	830:836	arg1	pasta					897:901	the cake-enriched pasta	879:901	the cake-enriched pasta	879:901	The content of polyphenols, including flavonoids, in the cake-enriched pasta increased significantly (p ≤ 0.05), which resulted in higher antioxidant activity against DPPH.
36234886	5	22	with	Pasta	738:742	arg1	GI					775:776	GI	775:776	GI	775:776	Pasta with a reduced glycemic index (GI) compared to the semolina control was obtained.
36234886	5	22	with	Pasta	738:742	arg1	index					768:772	a reduced glycemic index	749:772	a reduced glycemic index (GI) compared to the semolina control	749:810	Pasta with a reduced glycemic index (GI) compared to the semolina control was obtained.
36234886	2	23	theme	cumin	394:398	arg1	cake					400:403	black cumin cake	388:403	black cumin cake at the level of 5, 10, 15, 20, and 25%	388:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	4	24	theme	in	685:686	arg1	content					676:682	the digestible carbohydrate content	648:682	the digestible carbohydrate content	648:682	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	24	theme	in	685:686	arg1	HI					719:720	HI	719:720	HI	719:720	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	24	theme	in	685:686	arg1	index					712:716	in vitro starch hydrolysis index	685:716	in vitro starch hydrolysis index (HI)	685:721	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	2	25	theme	pasta	336:340	arg1	recipe					326:331	the recipe	322:331	the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%	322:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	2	26	theme	black	388:392	arg1	cake					400:403	black cumin cake	388:403	black cumin cake at the level of 5, 10, 15, 20, and 25%	388:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	7	27	from	times	1045:1049	arg1	sample					1065:1070	the sample	1061:1070	the sample with the 25% addition of black cumin cake	1061:1112	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	7	27	from	times	1045:1049	arg1	sample					1134:1139	the control sample	1122:1139	the control sample	1122:1139	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	7	28	theme	25	1081:1082	arg1	%					1083:1083	%	1083:1083	%	1083:1083	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	0	29	theme	Cumin	16:20	arg1	Addition					27:34	Black Cumin Cake Addition	10:34	Black Cumin Cake Addition	10:34	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	2	30	theme	%	442:442	arg1	level					412:416	the level	408:416	the level of 5, 10, 15, 20, and 25%	408:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	7	31	theme	black	1097:1101	arg1	cake					1109:1112	black cumin cake	1097:1112	black cumin cake	1097:1112	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	2	32	theme	study	259:263	arg1	purpose					244:250	The purpose	240:250	The purpose of the study	240:263	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	1	33	theme	good	156:159	arg1	carrier					161:167	a good carrier	154:167	a good carrier for plant enrichment substances due to its popularity among consumers	154:237	Pasta is a good carrier for plant enrichment substances due to its popularity among consumers.
36234886	1	33	theme	good	156:159	arg1	Pasta					145:149	Pasta	145:149	Pasta	145:149	Pasta is a good carrier for plant enrichment substances due to its popularity among consumers.
36234886	0	34	theme	Black	10:14	arg1	Addition					27:34	Black Cumin Cake Addition	10:34	Black Cumin Cake Addition	10:34	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	3	35	theme	≤	516:516	arg1	p					514:514	p ≤ 0.05	514:521	p ≤ 0.05	514:521	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	5	36	theme	glycemic	759:766	arg1	GI					775:776	GI	775:776	GI	775:776	Pasta with a reduced glycemic index (GI) compared to the semolina control was obtained.
36234886	5	36	theme	glycemic	759:766	arg1	index					768:772	a reduced glycemic index	749:772	a reduced glycemic index (GI) compared to the semolina control	749:810	Pasta with a reduced glycemic index (GI) compared to the semolina control was obtained.
36234886	8	37	theme	pasta	1259:1263	arg1	time					1251:1254	the cooking time	1239:1254	the cooking time of pasta	1239:1263	The functional addition significantly (p ≤ 0.05) increased the loss of dry matter and influenced the cooking time of pasta.
36234886	0	38	theme	Addition	27:34	arg1	Effect					0:5	Effect	0:5	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.	0:143	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	3	39	theme	fiber	574:578	arg1	content					540:546	the content	536:546	the content of protein, fat, ash, and fiber	536:578	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	7	40	with	sample	1065:1070	arg1	addition					1085:1092	the 25% addition	1077:1092	the 25% addition of black cumin cake	1077:1112	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	3	41	dep	fractions	622:630	arg1	both					591:594	both	591:594	both	591:594	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	3	42	theme	cake	468:471	arg1	use					449:451	The use	445:451	The use of black cumin cake	445:471	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	0	43	theme	Cake	22:25	arg1	Addition					27:34	Black Cumin Cake Addition	10:34	Black Cumin Cake Addition	10:34	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	3	44	theme	insoluble	600:608	arg1	fractions					622:630	both the insoluble and soluble fractions	591:630	fractions	622:630	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	0	45	theme	Cooking	107:113	arg1	Quality					115:121	Cooking Quality	107:121	Cooking Quality	107:121	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	3	46	theme	soluble	614:620	arg1	fractions					622:630	both the insoluble and soluble fractions	591:630	fractions	622:630	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	6	47	dep	increased	903:911	arg1	p					928:928	p ≤ 0.05	928:935	p ≤ 0.05	928:935	The content of polyphenols, including flavonoids, in the cake-enriched pasta increased significantly (p ≤ 0.05), which resulted in higher antioxidant activity against DPPH.
36234886	1	48	theme	plant	173:177	arg1	substances					190:199	plant enrichment substances	173:199	plant enrichment substances due to its popularity among consumers	173:237	Pasta is a good carrier for plant enrichment substances due to its popularity among consumers.
36234886	0	49	theme	Durum	126:130	arg1	Pasta					138:142	Durum Wheat Pasta	126:142	Durum Wheat Pasta	126:142	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	3	50	from	increase	524:531	arg1	content					540:546	the content	536:546	the content of protein, fat, ash, and fiber	536:578	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	3	51	theme	black	456:460	arg1	cake					468:471	black cumin cake	456:471	black cumin cake	456:471	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	3	52	theme	fat	560:562	arg1	content					540:546	the content	536:546	the content of protein, fat, ash, and fiber	536:578	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	2	53	theme	cake	400:403	arg1	addition					376:383	the addition	372:383	the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%	372:442	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	8	54	theme	functional	1146:1155	arg1	addition					1157:1164	The functional addition	1142:1164	The functional addition	1142:1164	The functional addition significantly (p ≤ 0.05) increased the loss of dry matter and influenced the cooking time of pasta.
36234886	3	55	theme	cumin	462:466	arg1	cake					468:471	black cumin cake	456:471	black cumin cake	456:471	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	2	56	theme	functional	288:297	arg1	potential					299:307	the functional potential	284:307	the functional potential	284:307	The purpose of the study was to investigate the functional potential and optimize the recipe of pasta made from durum semolina with the addition of black cumin cake at the level of 5, 10, 15, 20, and 25%.
36234886	6	57	theme	antioxidant	964:974	arg1	activity					976:983	higher antioxidant activity	957:983	higher antioxidant activity against DPPH	957:996	The content of polyphenols, including flavonoids, in the cake-enriched pasta increased significantly (p ≤ 0.05), which resulted in higher antioxidant activity against DPPH.
36234886	0	58	theme	Pasta	138:142	arg1	Quality					115:121	Cooking Quality	107:121	Cooking Quality	107:121	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	0	58	theme	Pasta	138:142	arg1	Composition					52:62	Chemical Composition	43:62	Chemical Composition	43:62	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	0	58	theme	Pasta	138:142	arg1	Index					74:78	Glycemic Index	65:78	Glycemic Index	65:78	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	0	58	theme	Pasta	138:142	arg1	Activity					93:100	Antioxidant Activity	81:100	Antioxidant Activity	81:100	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	7	59	theme	cumin	1103:1107	arg1	cake					1109:1112	black cumin cake	1097:1112	black cumin cake	1097:1112	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	4	60	from	reduction	635:643	arg1	content					676:682	the digestible carbohydrate content	648:682	the digestible carbohydrate content	648:682	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	4	60	from	reduction	635:643	arg1	index					712:716	in vitro starch hydrolysis index	685:716	in vitro starch hydrolysis index (HI)	685:721	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
36234886	8	61	dep	significantly	1166:1178	arg1	p					1181:1181	p ≤ 0.05	1181:1188	p ≤ 0.05	1181:1188	The functional addition significantly (p ≤ 0.05) increased the loss of dry matter and influenced the cooking time of pasta.
36234886	5	62	theme	reduced	751:757	arg1	GI					775:776	GI	775:776	GI	775:776	Pasta with a reduced glycemic index (GI) compared to the semolina control was obtained.
36234886	5	62	theme	reduced	751:757	arg1	index					768:772	a reduced glycemic index	749:772	a reduced glycemic index (GI) compared to the semolina control	749:810	Pasta with a reduced glycemic index (GI) compared to the semolina control was obtained.
36234886	1	63	theme	enrichment	179:188	arg1	substances					190:199	plant enrichment substances	173:199	plant enrichment substances due to its popularity among consumers	173:237	Pasta is a good carrier for plant enrichment substances due to its popularity among consumers.
36234886	0	64	theme	Wheat	132:136	arg1	Pasta					138:142	Durum Wheat Pasta	126:142	Durum Wheat Pasta	126:142	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	7	65	from	increase	1003:1010	arg1	content					1024:1030	the iron content	1015:1030	the iron content	1015:1030	The increase in the iron content was over 2.5 times higher in the sample with the 25% addition of black cumin cake than in the control sample.
36234886	3	66	theme	significant	501:511	arg1	increase					524:531	a statistically significant (p ≤ 0.05) increase	485:531	a statistically significant (p ≤ 0.05) increase	485:531	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	8	67	theme	matter	1217:1222	arg1	loss					1205:1208	the loss	1201:1208	the loss of dry matter	1201:1222	The functional addition significantly (p ≤ 0.05) increased the loss of dry matter and influenced the cooking time of pasta.
36234886	6	68	theme	cake-enriched	883:895	arg1	pasta					897:901	the cake-enriched pasta	879:901	the cake-enriched pasta	879:901	The content of polyphenols, including flavonoids, in the cake-enriched pasta increased significantly (p ≤ 0.05), which resulted in higher antioxidant activity against DPPH.
36234886	3	69	dep	significant	501:511	arg1	p					514:514	p ≤ 0.05	514:521	p ≤ 0.05	514:521	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	6	70	theme	polyphenols	841:851	arg1	content					830:836	The content	826:836	The content of polyphenols, including flavonoids, in the cake-enriched pasta	826:901	The content of polyphenols, including flavonoids, in the cake-enriched pasta increased significantly (p ≤ 0.05), which resulted in higher antioxidant activity against DPPH.
36234886	1	71	theme	due	201:203	arg1	substances					190:199	plant enrichment substances	173:199	plant enrichment substances due to its popularity among consumers	173:237	Pasta is a good carrier for plant enrichment substances due to its popularity among consumers.
36234886	0	72	theme	Chemical	43:50	arg1	Composition					52:62	Chemical Composition	43:62	Chemical Composition	43:62	Effect of Black Cumin Cake Addition on the Chemical Composition, Glycemic Index, Antioxidant Activity, and Cooking Quality of Durum Wheat Pasta.
36234886	3	73	theme	protein	551:557	arg1	content					540:546	the content	536:546	the content of protein, fat, ash, and fiber	536:578	The use of black cumin cake resulted in a statistically significant (p ≤ 0.05) increase in the content of protein, fat, ash, and fiber, including both the insoluble and soluble fractions.
36234886	5	74	theme	semolina	795:802	arg1	control					804:810	the semolina control	791:810	the semolina control	791:810	Pasta with a reduced glycemic index (GI) compared to the semolina control was obtained.
36234886	4	75	dep	in	685:686	arg1	vitro					688:692	vitro	688:692	vitro	688:692	A reduction in the digestible carbohydrate content, in vitro starch hydrolysis index (HI), was observed.
34932869	4	0	theme	tissues	727:733	arg1	analysis					707:714	RNAseq analysis	700:714	RNAseq analysis of colonic tissues	700:733	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	5	1	theme	indole	1098:1103	arg1	propionate					1105:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	7	2	theme	potential	1358:1366	arg1	role					1378:1381	a potential mediating role	1356:1381	a potential mediating role of gut microbiome	1356:1399	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	3	3	theme	lower	482:486	arg1	score					501:505	significantly lower histological score	468:505	significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and	468:597	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	7	4	theme	microbiome	1390:1399	arg1	effects					1304:1310	the anti-inflammatory effects	1282:1310	the anti-inflammatory effects of dietary PomX supplementation for IBD	1282:1350	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	7	4	theme	microbiome	1390:1399	arg1	role					1378:1381	a potential mediating role	1356:1381	a potential mediating role of gut microbiome	1356:1399	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	4	5	theme	RNAseq	700:705	arg1	analysis					707:714	RNAseq analysis	700:714	RNAseq analysis of colonic tissues	700:733	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	6	6	theme	PomX	1162:1165	arg1	supplementation					1167:1181	dietary PomX supplementation	1154:1181	dietary PomX supplementation	1154:1181	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	1	7	theme	extract	156:162	arg1	supplementation					171:185	dietary pomegranate extract (PomX) supplementation	136:185	dietary pomegranate extract (PomX) supplementation	136:185	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	3	8	theme	Interleukin	617:627	arg1	15.8±2.2 vs 29.5±5.5					645:664	15.8±2.2 vs 29.5±5.5	645:664	15.8±2.2 vs 29.5±5.5	645:664	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	8	theme	Interleukin	617:627	arg1	levels					637:642	circulating Interleukin 6(IL-6) levels	605:642	circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5)	605:665	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	1	9	theme	PomX	165:168	arg1	supplementation					171:185	dietary pomegranate extract (PomX) supplementation	136:185	dietary pomegranate extract (PomX) supplementation	136:185	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	7	10	theme	PomX	1323:1326	arg1	supplementation					1328:1342	dietary PomX supplementation	1315:1342	dietary PomX supplementation for IBD	1315:1350	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	6	11	theme	fed	1244:1246	arg1	mice					1257:1260	HFHS fed IL-10-/- mice	1239:1260	HFHS fed IL-10-/- mice	1239:1260	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	5	12	theme	cecal	950:954	arg1	microbiome					956:965	the cecal microbiome	946:965	the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate	946:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	7	13	theme	anti-inflammatory	1286:1302	arg1	effects					1304:1310	the anti-inflammatory effects	1282:1310	the anti-inflammatory effects of dietary PomX supplementation for IBD	1282:1350	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	2	14	dep	METHODS	270:276	arg1	assigned					336:343	assigned	336:343	are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks	323:436	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	4	15	theme	upregulated	768:778	arg1	genes					780:784	483 downregulated and 263 upregulated genes	742:784	483 downregulated and 263 upregulated genes	742:784	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	6	16	theme	inflammatory	1215:1226	arg1	markers					1228:1234	inflammatory markers	1215:1234	inflammatory markers in HFHS fed IL-10-/- mice	1215:1260	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	5	17	theme	PomX	923:926	arg1	treatment					928:936	PomX treatment	923:936	PomX treatment	923:936	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	2	18	with	supplement	399:408	arg1	PomX					421:424	0.25% PomX	415:424	0.25% PomX for 8 weeks	415:436	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	5	19	theme	propionate	1105:1114	arg1	levels					1044:1049	increased levels	1034:1049	increased levels of the tryptophan-related microbial metabolite indole propionate	1034:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	5	19	theme	propionate	1105:1114	arg1	diversity					988:996	increased alpha diversity	972:996	increased alpha diversity	972:996	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	5	19	theme	propionate	1105:1114	arg1	composition					1017:1027	altered microbial composition	999:1027	altered microbial composition	999:1027	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	3	20	dep	lower	599:603	arg1	15.8±2.2 vs 29.5±5.5					645:664	15.8±2.2 vs 29.5±5.5	645:664	15.8±2.2 vs 29.5±5.5	645:664	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	20	dep	lower	599:603	arg1	levels					637:642	circulating Interleukin 6(IL-6) levels	605:642	circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5)	605:665	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	7	21	theme	IBD	1504:1506	arg1	patients					1508:1515	IBD patients	1504:1515	IBD patients	1504:1515	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	1	22	theme	murine	256:261	arg1	model					263:267	IL-10 deficient (IL-10-/-) murine model	229:267	IL-10 deficient (IL-10-/-) murine model	229:267	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	3	23	theme	fed	686:688	arg1	controls					690:697	HFHS fed controls	681:697	HFHS fed controls	681:697	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	0	24	theme	High	66:69	arg1	Diet					88:91	a High Fat High Sucrose Diet	64:91	a High Fat High Sucrose Diet	64:91	Pomegranate Extract Improves Colitis in IL-10 Knockout Mice Fed a High Fat High Sucrose Diet.
34932869	5	25	theme	microbial	1007:1015	arg1	composition					1017:1027	altered microbial composition	999:1027	altered microbial composition	999:1027	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	1	26	theme	dietary	136:142	arg1	supplementation					171:185	dietary pomegranate extract (PomX) supplementation	136:185	dietary pomegranate extract (PomX) supplementation	136:185	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	7	27	theme	supplementation	1485:1499	arg1	use					1465:1467	the use	1461:1467	the use of PomX dietary supplementation in IBD patients	1461:1515	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	0	28	theme	High	75:78	arg1	Diet					88:91	a High Fat High Sucrose Diet	64:91	a High Fat High Sucrose Diet	64:91	Pomegranate Extract Improves Colitis in IL-10 Knockout Mice Fed a High Fat High Sucrose Diet.
34932869	7	29	theme	PomX	1472:1475	arg1	supplementation					1485:1499	PomX dietary supplementation	1472:1499	PomX dietary supplementation	1472:1499	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	1	30	dep	SCOPE	94:98	arg1	feed					224:227	feed	224:227	feed IL-10 deficient (IL-10-/-) murine model	224:267	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	2	31	theme	high	359:362	arg1	diet					379:382	a high fat high sucrose (HFHS) diet	348:382	a high fat high sucrose (HFHS) diet	348:382	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	5	32	theme	alpha	982:986	arg1	diversity					988:996	increased alpha diversity	972:996	increased alpha diversity	972:996	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	2	33	theme	HFHS	389:392	arg1	diet					394:397	a HFHS diet	387:397	a HFHS diet supplement with 0.25% PomX for 8 weeks	387:436	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	2	34	theme	high	350:353	arg1	diet					379:382	a high fat high sucrose (HFHS) diet	348:382	a high fat high sucrose (HFHS) diet	348:382	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	0	35	theme	Pomegranate	0:10	arg1	Extract					12:18	Pomegranate Extract	0:18	Pomegranate Extract	0:18	Pomegranate Extract Improves Colitis in IL-10 Knockout Mice Fed a High Fat High Sucrose Diet.
34932869	5	36	with	microbiome	956:965	arg1	levels					1044:1049	increased levels	1034:1049	increased levels of the tryptophan-related microbial metabolite indole propionate	1034:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	5	36	with	microbiome	956:965	arg1	diversity					988:996	increased alpha diversity	972:996	increased alpha diversity	972:996	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	5	36	with	microbiome	956:965	arg1	composition					1017:1027	altered microbial composition	999:1027	altered microbial composition	999:1027	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	5	37	theme	tryptophan-related	1058:1075	arg1	propionate					1105:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	3	38	dep	colitis	511:517	arg1	±					524:524	2.6 ± 0.5 vs 3.9 ± 1.0	520:541	±	524:524	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	38	dep	colitis	511:517	arg1	±					537:537	2.6 ± 0.5 vs 3.9 ± 1.0	520:541	±	537:537	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	2	39	theme	0.25	415:418	arg1	%					419:419	%	419:419	%	419:419	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	3	40	theme	spleen	551:556	arg1	colitis					511:517	colitis	511:517	colitis (2.6 ± 0.5 vs 3.9 ± 1.0)	511:542	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	40	theme	spleen	551:556	arg1	weight					558:563	lower spleen weight	545:563	lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02)	545:592	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	40	theme	spleen	551:556	arg1	±					571:571	0.11 ± 0.01 vs 0.15 ± 0.02	566:591	0.11 ± 0.01 vs 0.15 ± 0.02	566:591	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	6	41	from	markers	1228:1234	arg1	mice					1257:1260	HFHS fed IL-10-/- mice	1239:1260	HFHS fed IL-10-/- mice	1239:1260	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	2	42	theme	supplement	399:408	arg1	diet					394:397	a HFHS diet	387:397	a HFHS diet supplement with 0.25% PomX for 8 weeks	387:436	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	0	43	theme	Knockout	46:53	arg1	Mice					55:58	IL-10 Knockout Mice	40:58	IL-10 Knockout Mice Fed a High Fat High Sucrose Diet	40:91	Pomegranate Extract Improves Colitis in IL-10 Knockout Mice Fed a High Fat High Sucrose Diet.
34932869	5	44	theme	increased	1034:1042	arg1	levels					1044:1049	increased levels	1034:1049	increased levels of the tryptophan-related microbial metabolite indole propionate	1034:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	2	45	theme	male	304:307	arg1	mice					318:321	Four-week-old male IL-10-/- mice	290:321	Four-week-old male IL-10-/- mice	290:321	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	3	46	theme	PomX	439:442	arg1	supplementation					444:458	PomX supplementation	439:458	PomX supplementation	439:458	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	1	47	theme	Western	211:217	arg1	diet					219:222	a Western diet	209:222	a Western diet	209:222	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	5	48	theme	metabolite	1087:1096	arg1	propionate					1105:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	4	49	theme	colonic	719:725	arg1	tissues					727:733	colonic tissues	719:733	colonic tissues	719:733	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	1	50	theme	deficient	235:243	arg1	model					263:267	IL-10 deficient (IL-10-/-) murine model	229:267	IL-10 deficient (IL-10-/-) murine model	229:267	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	7	51	theme	gut	1386:1388	arg1	microbiome					1390:1399	gut microbiome	1386:1399	gut microbiome	1386:1399	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	7	52	theme	mediating	1368:1376	arg1	role					1378:1381	a potential mediating role	1356:1381	a potential mediating role of gut microbiome	1356:1399	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	1	53	theme	pomegranate	144:154	arg1	supplementation					171:185	dietary pomegranate extract (PomX) supplementation	136:185	dietary pomegranate extract (PomX) supplementation	136:185	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	7	54	from	use	1465:1467	arg1	patients					1508:1515	IBD patients	1504:1515	IBD patients	1504:1515	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	4	55	theme	inflammatory	846:857	arg1	responses					859:867	inflammatory responses	846:867	inflammatory responses	846:867	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	6	56	theme	dietary	1154:1160	arg1	supplementation					1167:1181	dietary PomX supplementation	1154:1181	dietary PomX supplementation	1154:1181	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	3	57	theme	histological	488:499	arg1	score					501:505	significantly lower histological score	468:505	significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and	468:597	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	7	58	theme	supplementation	1328:1342	arg1	effects					1304:1310	the anti-inflammatory effects	1282:1310	the anti-inflammatory effects of dietary PomX supplementation for IBD	1282:1350	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	7	58	theme	supplementation	1328:1342	arg1	role					1378:1381	a potential mediating role	1356:1381	a potential mediating role of gut microbiome	1356:1399	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	3	59	theme	circulating	605:615	arg1	15.8±2.2 vs 29.5±5.5					645:664	15.8±2.2 vs 29.5±5.5	645:664	15.8±2.2 vs 29.5±5.5	645:664	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	59	theme	circulating	605:615	arg1	levels					637:642	circulating Interleukin 6(IL-6) levels	605:642	circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5)	605:665	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	6	60	theme	IL-10-/-	1248:1255	arg1	mice					1257:1260	HFHS fed IL-10-/- mice	1239:1260	HFHS fed IL-10-/- mice	1239:1260	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	4	61	theme	PomX	791:794	arg1	supplementation					796:810	PomX supplementation	791:810	PomX supplementation	791:810	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	7	62	theme	dietary	1315:1321	arg1	supplementation					1328:1342	dietary PomX supplementation	1315:1342	dietary PomX supplementation for IBD	1315:1350	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	6	63	theme	HFHS	1239:1242	arg1	mice					1257:1260	HFHS fed IL-10-/- mice	1239:1260	HFHS fed IL-10-/- mice	1239:1260	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	4	64	theme	downregulated	746:758	arg1	genes					780:784	483 downregulated and 263 upregulated genes	742:784	483 downregulated and 263 upregulated genes	742:784	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	1	65	dep	deficient	235:243	arg1	IL-10-/-					246:253	IL-10-/-	246:253	IL-10-/-	246:253	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	1	66	theme	study	104:108	arg1	tests					110:114	The study tests	100:114	The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet	100:222	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	4	67	dep	upregulated	768:778	arg1	263					764:766	263	764:766	263	764:766	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	6	68	dep	CONCLUSION	1117:1126	arg1	demonstrate					1137:1147	demonstrate	1137:1147	demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice	1137:1260	CONCLUSION The data demonstrate that dietary PomX supplementation ameliorated colitis and lowered inflammatory markers in HFHS fed IL-10-/- mice.
34932869	0	69	theme	Fat	71:73	arg1	Diet					88:91	a High Fat High Sucrose Diet	64:91	a High Fat High Sucrose Diet	64:91	Pomegranate Extract Improves Colitis in IL-10 Knockout Mice Fed a High Fat High Sucrose Diet.
34932869	5	70	theme	altered	999:1005	arg1	composition					1017:1027	altered microbial composition	999:1027	altered microbial composition	999:1027	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	7	71	theme	dietary	1477:1483	arg1	supplementation					1485:1499	PomX dietary supplementation	1472:1499	PomX dietary supplementation	1472:1499	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	2	72	theme	%	419:419	arg1	PomX					421:424	0.25% PomX	415:424	0.25% PomX for 8 weeks	415:436	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	0	73	theme	Sucrose	80:86	arg1	Diet					88:91	a High Fat High Sucrose Diet	64:91	a High Fat High Sucrose Diet	64:91	Pomegranate Extract Improves Colitis in IL-10 Knockout Mice Fed a High Fat High Sucrose Diet.
34932869	1	74	dep	tests	110:114	arg1	hypothesis					120:129	the hypothesis	116:129	The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet	100:222	SCOPE The study tests the hypothesis that dietary pomegranate extract (PomX) supplementation attenuates colitis in a Western diet feed IL-10 deficient (IL-10-/-) murine model.
34932869	3	75	theme	HFHS	681:684	arg1	controls					690:697	HFHS fed controls	681:697	HFHS fed controls	681:697	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	7	76	dep	future	1426:1431	arg1	clinical					1433:1440	clinical	1433:1440	clinical	1433:1440	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
34932869	5	77	theme	increased	972:980	arg1	diversity					988:996	increased alpha diversity	972:996	increased alpha diversity	972:996	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	2	78	theme	fat	355:357	arg1	diet					379:382	a high fat high sucrose (HFHS) diet	348:382	a high fat high sucrose (HFHS) diet	348:382	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	4	79	theme	neutrophil	884:893	arg1	degranulation					895:907	neutrophil degranulation	884:907	neutrophil degranulation	884:907	RNAseq analysis of colonic tissues showed 483 downregulated and 263 upregulated genes with PomX supplementation, which are mainly associated with inflammatory responses, defenses, and neutrophil degranulation.
34932869	3	80	theme	lower	545:549	arg1	colitis					511:517	colitis	511:517	colitis (2.6 ± 0.5 vs 3.9 ± 1.0)	511:542	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	80	theme	lower	545:549	arg1	weight					558:563	lower spleen weight	545:563	lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02)	545:592	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	3	80	theme	lower	545:549	arg1	±					571:571	0.11 ± 0.01 vs 0.15 ± 0.02	566:591	0.11 ± 0.01 vs 0.15 ± 0.02	566:591	PomX supplementation lead to significantly lower histological score for colitis (2.6 ± 0.5 vs 3.9 ± 1.0), lower spleen weight (0.11 ± 0.01 vs 0.15 ± 0.02), and lower circulating Interleukin 6(IL-6) levels (15.8±2.2 vs 29.5±5.5) compared with HFHS fed controls.
34932869	2	81	theme	HFHS	373:376	arg1	diet					379:382	a high fat high sucrose (HFHS) diet	348:382	a high fat high sucrose (HFHS) diet	348:382	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	0	82	theme	IL-10	40:44	arg1	Mice					55:58	IL-10 Knockout Mice	40:58	IL-10 Knockout Mice Fed a High Fat High Sucrose Diet	40:91	Pomegranate Extract Improves Colitis in IL-10 Knockout Mice Fed a High Fat High Sucrose Diet.
34932869	5	83	theme	microbial	1077:1085	arg1	propionate					1105:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	the tryptophan-related microbial metabolite indole propionate	1054:1114	In addition, PomX treatment affects the cecal microbiome with increased alpha diversity, altered microbial composition, and increased levels of the tryptophan-related microbial metabolite indole propionate.
34932869	2	84	theme	sucrose	364:370	arg1	diet					379:382	a high fat high sucrose (HFHS) diet	348:382	a high fat high sucrose (HFHS) diet	348:382	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	2	85	theme	IL-10-/-	309:316	arg1	mice					318:321	Four-week-old male IL-10-/- mice	290:321	Four-week-old male IL-10-/- mice	290:321	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	2	86	theme	Four-week-old	290:302	arg1	mice					318:321	Four-week-old male IL-10-/- mice	290:321	Four-week-old male IL-10-/- mice	290:321	METHODS AND RESULTS Four-week-old male IL-10-/- mice are randomly assigned to a high fat high sucrose (HFHS) diet or a HFHS diet supplement with 0.25% PomX for 8 weeks.
34932869	7	87	theme	future	1426:1431	arg1	studies					1442:1448	future clinical studies	1426:1448	future clinical studies	1426:1448	These data support the anti-inflammatory effects of dietary PomX supplementation for IBD and a potential mediating role of gut microbiome, suggesting the need for future clinical studies to explore the use of PomX dietary supplementation in IBD patients.
36634380	10	0	theme	rRNA	1478:1481	arg1	sequencing					1483:1492	rRNA sequencing	1478:1492	rRNA sequencing	1478:1492	Finally, the changes of gut microbiota composition were analyzed by 16S rRNA sequencing.
36634380	4	1	from	effects	501:507	arg1	interactions					554:565	inflammation interactions	541:565	inflammation interactions	541:565	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	4	1	from	effects	501:507	arg1	synthesis					576:584	5-HT synthesis	571:584	5-HT synthesis	571:584	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	9	2	theme	surface	1373:1379	arg1	SPR					1400:1402	SPR	1400:1402	SPR	1400:1402	The interaction between corylin and 5-hydroxytryptophan decarboxylase (5-HTPDC) was performed by molecular docking and surface plasmon resonance (SPR).
36634380	9	2	theme	surface	1373:1379	arg1	resonance					1389:1397	surface plasmon resonance	1373:1397	surface plasmon resonance (SPR)	1373:1403	The interaction between corylin and 5-hydroxytryptophan decarboxylase (5-HTPDC) was performed by molecular docking and surface plasmon resonance (SPR).
36634380	11	3	theme	mice	1638:1641	arg1	brain					1606:1610	brain	1606:1610	brain	1606:1610	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	11	3	theme	mice	1638:1641	arg1	colon					1596:1600	colon	1596:1600	colon	1596:1600	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	7	4	theme	western	1060:1066	arg1	blotting					1068:1075	western blotting	1060:1075	western blotting	1060:1075	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	1	5	theme	common	257:262	arg1	disorders					271:279	common mental disorders	257:279	common mental disorders	257:279	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	8	6	theme	tryptophan	1131:1140	arg1	metabolites					1148:1158	several tryptophan (Trp) metabolites	1123:1158	several tryptophan (Trp) metabolites	1123:1158	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	7	from	neurotransmitters	1172:1188	arg1	colon					1201:1205	colon	1201:1205	colon	1201:1205	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	7	from	neurotransmitters	1172:1188	arg1	faeces					1193:1198	faeces	1193:1198	faeces	1193:1198	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	7	from	neurotransmitters	1172:1188	arg1	serum					1208:1212	serum	1208:1212	serum	1208:1212	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	7	from	neurotransmitters	1172:1188	arg1	brain					1219:1223	brain	1219:1223	brain	1219:1223	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	11	8	theme	chronic	1627:1633	arg1	mice					1638:1641	DSS-induced chronic UC mice	1615:1641	DSS-induced chronic UC mice	1615:1641	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	8	9	theme	Trp	1143:1145	arg1	metabolites					1148:1158	several tryptophan (Trp) metabolites	1123:1158	several tryptophan (Trp) metabolites	1123:1158	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	3	10	attach	isolated	431:438	arg1	Fructus					459:465	the Psoraleae Fructus	445:465	the Psoraleae Fructus	445:465	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	3	10	attach	isolated	431:438	arg2	Corylin					384:390	PURPOSE Corylin	376:390	PURPOSE Corylin	376:390	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	3	10	attach	isolated	431:438	arg2	compound					422:429	a representative flavonoid compound	395:429	a representative flavonoid compound isolated from the Psoraleae Fructus	395:465	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	5	11	theme	sulfate	643:649	arg1	DSS					659:661	DSS	659:661	DSS	659:661	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	5	11	theme	sulfate	643:649	arg1	sodium					651:656	Dextran sulfate sodium	635:656	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model	627:693	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	5	12	theme	mouse	683:687	arg1	model					689:693	chronic UC mouse model	672:693	chronic UC mouse model	672:693	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	2	13	theme	increasing	286:295	arg1	interest					297:304	The increasing interest	282:304	The increasing interest in the role of gut-brain axis	282:334	The increasing interest in the role of gut-brain axis is seen in inflammatory bowel diseases.
36634380	8	14	theme	related	1164:1170	arg1	neurotransmitters					1172:1188	related neurotransmitters	1164:1188	related neurotransmitters	1164:1188	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	16	15	theme	gut-brain	2625:2633	arg1	axis					2635:2638	gut-brain axis	2625:2638	gut-brain axis	2625:2638	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	15	16	theme	microbiota	2293:2302	arg1	composition					2267:2277	composition	2267:2277	composition	2267:2277	Moreover, the intake of corylin transformed the diversity and composition of intestinal microbiota, Bacteroides, Escherichia-Shigella, and Turicibacter were decreased but Dubosiella, Enterorhabdus, and Candidatus_Stoquefichus were increased.
36634380	15	16	theme	microbiota	2293:2302	arg1	diversity					2253:2261	diversity	2253:2261	diversity	2253:2261	Moreover, the intake of corylin transformed the diversity and composition of intestinal microbiota, Bacteroides, Escherichia-Shigella, and Turicibacter were decreased but Dubosiella, Enterorhabdus, and Candidatus_Stoquefichus were increased.
36634380	1	17	contain	have	231:234	arg2	prevalence					243:252	a high prevalence	236:252	a high prevalence of common mental disorders	236:279	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	17	contain	have	231:234	arg1	patients					206:213	patients	206:213	patients with chronic UC	206:229	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	12	18	theme	mucosal	1674:1680	arg1	barrier					1682:1688	intestinal mucosal barrier	1663:1688	intestinal mucosal barrier	1663:1688	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	16	19	theme	5-HTP	2655:2659	arg1	generation					2661:2670	5-HTP generation	2655:2670	5-HTP generation in the colon	2655:2683	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	7	20	theme	adaptor	996:1002	arg1	molecule					1004:1011	the ionized calcium-binding adaptor molecule 1	968:1013	the ionized calcium-binding adaptor molecule 1 (Iba1)	968:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	7	20	theme	adaptor	996:1002	arg1	Iba1					1016:1019	Iba1	1016:1019	Iba1	1016:1019	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	1	21	theme	Chronic	151:157	arg1	colitis					170:176	BACKGROUND Chronic ulcerative colitis	140:176	BACKGROUND Chronic ulcerative colitis (UC)	140:181	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	21	theme	Chronic	151:157	arg1	disease					197:203	a lifelong disease	186:203	a lifelong disease	186:203	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	21	theme	Chronic	151:157	arg1	UC					179:180	UC	179:180	UC	179:180	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	16	22	theme	intestinal	2527:2536	arg1	inflammation					2538:2549	intestinal inflammation	2527:2549	intestinal inflammation	2527:2549	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	4	23	from	brain	606:610	arg1	UC					623:624	chronic UC	615:624	chronic UC	615:624	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	7	24	theme	blood-brain	938:948	arg1	barrier					950:956	blood-brain barrier	938:956	blood-brain barrier	938:956	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	2	25	from	interest	297:304	arg1	role					313:316	the role	309:316	the role of gut-brain axis	309:334	The increasing interest in the role of gut-brain axis is seen in inflammatory bowel diseases.
36634380	5	26	theme	chronic	672:678	arg1	model					689:693	chronic UC mouse model	672:693	chronic UC mouse model	672:693	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	4	27	from	gut	598:600	arg1	UC					623:624	chronic UC	615:624	chronic UC	615:624	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	7	28	theme	barrier	926:932	arg1	proteins					895:902	tight junction (TJ) proteins	875:902	tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1)	875:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	0	29	theme	5-hydroxytryptophan	95:113	arg1	production					115:124	5-hydroxytryptophan production	95:124	5-hydroxytryptophan production in the colon	95:137	Corylin ameliorates chronic ulcerative colitis via regulating the gut-brain axis and promoting 5-hydroxytryptophan production in the colon.
36634380	7	30	theme	intestinal	907:916	arg1	BBB					959:961	BBB	959:961	BBB	959:961	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	7	30	theme	intestinal	907:916	arg1	barrier					926:932	intestinal mucosal barrier	907:932	intestinal mucosal barrier	907:932	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	14	31	dep	reduction	2158:2166	arg1	the					2149:2151	the	2149:2151	the	2149:2151	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36634380	7	32	theme	proteins	895:902	arg1	expression					861:870	The expression	857:870	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus	857:1039	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	3	33	theme	flavonoid	412:420	arg1	compound					422:429	a representative flavonoid compound	395:429	a representative flavonoid compound isolated from the Psoraleae Fructus	395:465	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	3	33	theme	flavonoid	412:420	arg1	Corylin					384:390	PURPOSE Corylin	376:390	PURPOSE Corylin	376:390	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	14	34	theme	Further	2015:2021	arg1	study					2023:2027	Further study	2015:2027	Further study	2015:2027	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36634380	1	35	theme	mental	264:269	arg1	disorders					271:279	common mental disorders	257:279	common mental disorders	257:279	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	4	36	dep	gut	598:600	arg1	the					594:596	the	594:596	the	594:596	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	10	37	theme	composition	1445:1455	arg1	changes					1419:1425	the changes	1415:1425	the changes of gut microbiota composition	1415:1455	Finally, the changes of gut microbiota composition were analyzed by 16S rRNA sequencing.
36634380	0	38	theme	chronic	20:26	arg1	colitis					39:45	chronic ulcerative colitis	20:45	chronic ulcerative colitis	20:45	Corylin ameliorates chronic ulcerative colitis via regulating the gut-brain axis and promoting 5-hydroxytryptophan production in the colon.
36634380	9	39	theme	5-hydroxytryptophan	1290:1308	arg1	5-HTPDC					1325:1331	5-HTPDC	1325:1331	5-HTPDC	1325:1331	The interaction between corylin and 5-hydroxytryptophan decarboxylase (5-HTPDC) was performed by molecular docking and surface plasmon resonance (SPR).
36634380	9	39	theme	5-hydroxytryptophan	1290:1308	arg1	decarboxylase					1310:1322	5-hydroxytryptophan decarboxylase	1290:1322	5-hydroxytryptophan decarboxylase (5-HTPDC)	1290:1332	The interaction between corylin and 5-hydroxytryptophan decarboxylase (5-HTPDC) was performed by molecular docking and surface plasmon resonance (SPR).
36634380	8	40	located	detected	1230:1237	arg2	neurotransmitters					1172:1188	related neurotransmitters	1164:1188	related neurotransmitters	1164:1188	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	40	located	detected	1230:1237	arg2	metabolites					1148:1158	several tryptophan (Trp) metabolites	1123:1158	several tryptophan (Trp) metabolites	1123:1158	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	40	located	detected	1230:1237	arg1	addition					1113:1120	addition	1113:1120	addition	1113:1120	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	16	41	theme	Corylin	2458:2464	arg1	administration					2466:2479	CONCLUSION Corylin administration	2447:2479	CONCLUSION Corylin administration	2447:2479	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	10	42	theme	gut	1430:1432	arg1	composition					1445:1455	gut microbiota composition	1430:1455	gut microbiota composition	1430:1455	Finally, the changes of gut microbiota composition were analyzed by 16S rRNA sequencing.
36634380	5	43	theme	chronic	758:764	arg1	symptoms					769:776	chronic UC symptoms	758:776	chronic UC symptoms	758:776	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	3	44	theme	representative	397:410	arg1	compound					422:429	a representative flavonoid compound	395:429	a representative flavonoid compound isolated from the Psoraleae Fructus	395:465	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	3	44	theme	representative	397:410	arg1	Corylin					384:390	PURPOSE Corylin	376:390	PURPOSE Corylin	376:390	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	14	45	from	accumulation	2178:2189	arg1	colon					2198:2202	the colon	2194:2202	the colon	2194:2202	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36634380	0	46	theme	gut-brain	66:74	arg1	axis					76:79	the gut-brain axis	62:79	the gut-brain axis	62:79	Corylin ameliorates chronic ulcerative colitis via regulating the gut-brain axis and promoting 5-hydroxytryptophan production in the colon.
36634380	11	47	theme	inflammatory	1567:1578	arg1	response					1580:1587	inflammatory response	1567:1587	inflammatory response in the colon and brain of DSS-induced chronic UC mice	1567:1641	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	2	48	theme	axis	331:334	arg1	role					313:316	the role	309:316	the role of gut-brain axis	309:334	The increasing interest in the role of gut-brain axis is seen in inflammatory bowel diseases.
36634380	4	49	from	mechanism	513:521	arg1	interactions					554:565	inflammation interactions	541:565	inflammation interactions	541:565	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	4	49	from	mechanism	513:521	arg1	synthesis					576:584	5-HT synthesis	571:584	5-HT synthesis	571:584	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	6	50	dep	colon	840:844	arg1	the					836:838	the	836:838	the	836:838	The expression of inflammatory cytokines was detected in the colon and brain.
36634380	1	51	theme	chronic	220:226	arg1	UC					228:229	chronic UC	220:229	chronic UC	220:229	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	6	52	theme	cytokines	810:818	arg1	expression					783:792	The expression	779:792	The expression of inflammatory cytokines	779:818	The expression of inflammatory cytokines was detected in the colon and brain.
36634380	1	53	dep	disease	197:203	arg1	have					231:234	have	231:234	have a high prevalence of common mental disorders	231:279	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	12	54	theme	TJ	1648:1649	arg1	proteins					1651:1658	The TJ proteins	1644:1658	The TJ proteins of intestinal mucosal barrier and BBB	1644:1696	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	2	55	theme	inflammatory	347:358	arg1	diseases					366:373	inflammatory bowel diseases	347:373	inflammatory bowel diseases	347:373	The increasing interest in the role of gut-brain axis is seen in inflammatory bowel diseases.
36634380	7	56	theme	tight	875:879	arg1	TJ					891:892	TJ	891:892	TJ	891:892	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	7	56	theme	tight	875:879	arg1	junction					881:888	tight junction	875:888	tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1)	875:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	9	57	theme	molecular	1351:1359	arg1	docking					1361:1367	molecular docking	1351:1367	molecular docking	1351:1367	The interaction between corylin and 5-hydroxytryptophan decarboxylase (5-HTPDC) was performed by molecular docking and surface plasmon resonance (SPR).
36634380	5	58	theme	UC	766:767	arg1	symptoms					769:776	chronic UC symptoms	758:776	chronic UC symptoms	758:776	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	8	59	from	metabolites	1148:1158	arg1	colon					1201:1205	colon	1201:1205	colon	1201:1205	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	59	from	metabolites	1148:1158	arg1	faeces					1193:1198	faeces	1193:1198	faeces	1193:1198	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	59	from	metabolites	1148:1158	arg1	serum					1208:1212	serum	1208:1212	serum	1208:1212	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	8	59	from	metabolites	1148:1158	arg1	brain					1219:1223	brain	1219:1223	brain	1219:1223	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	11	60	theme	DSS-induced	1615:1625	arg1	mice					1638:1641	DSS-induced chronic UC mice	1615:1641	DSS-induced chronic UC mice	1615:1641	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	5	61	theme	Dextran	635:641	arg1	DSS					659:661	DSS	659:661	DSS	659:661	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	5	61	theme	Dextran	635:641	arg1	sodium					651:656	Dextran sulfate sodium	635:656	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model	627:693	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	11	62	theme	UC	1635:1636	arg1	mice					1638:1641	DSS-induced chronic UC mice	1615:1641	DSS-induced chronic UC mice	1615:1641	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	1	63	theme	high	238:241	arg1	prevalence					243:252	a high prevalence	236:252	a high prevalence of common mental disorders	236:279	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	4	64	theme	5-HT	571:574	arg1	synthesis					576:584	5-HT synthesis	571:584	5-HT synthesis	571:584	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	12	65	theme	BBB	1694:1696	arg1	proteins					1651:1658	The TJ proteins	1644:1658	The TJ proteins of intestinal mucosal barrier and BBB	1644:1696	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	11	66	theme	RESULTS	1495:1501	arg1	Corylin					1503:1509	RESULTS Corylin	1495:1509	RESULTS Corylin	1495:1509	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	9	67	theme	plasmon	1381:1387	arg1	SPR					1400:1402	SPR	1400:1402	SPR	1400:1402	The interaction between corylin and 5-hydroxytryptophan decarboxylase (5-HTPDC) was performed by molecular docking and surface plasmon resonance (SPR).
36634380	9	67	theme	plasmon	1381:1387	arg1	resonance					1389:1397	surface plasmon resonance	1373:1397	surface plasmon resonance (SPR)	1373:1403	The interaction between corylin and 5-hydroxytryptophan decarboxylase (5-HTPDC) was performed by molecular docking and surface plasmon resonance (SPR).
36634380	12	68	theme	Iba1	1734:1737	arg1	expression					1720:1729	the expression	1716:1729	the expression of Iba1 in the hippocampus	1716:1756	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	14	69	theme	VB6	2131:2133	arg1	expression					2105:2114	the expression	2101:2114	the expression of 5-HTDPC and VB6	2101:2133	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36634380	16	70	from	generation	2661:2670	arg1	colon					2679:2683	the colon	2675:2683	the colon	2675:2683	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	11	71	from	response	1580:1587	arg1	brain					1606:1610	brain	1606:1610	brain	1606:1610	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	11	71	from	response	1580:1587	arg1	colon					1596:1600	colon	1596:1600	colon	1596:1600	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	8	72	theme	several	1123:1129	arg1	metabolites					1148:1158	several tryptophan (Trp) metabolites	1123:1158	several tryptophan (Trp) metabolites	1123:1158	In addition, several tryptophan (Trp) metabolites and related neurotransmitters in faeces, colon, serum, and brain were detected by UPLC-MS/MS.
36634380	14	73	theme	5-HTDPC	2119:2125	arg1	expression					2105:2114	the expression	2101:2114	the expression of 5-HTDPC and VB6	2101:2133	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36634380	5	74	theme	corylin	747:753	arg1	effect					737:742	the therapeutic effect	721:742	the therapeutic effect of corylin on chronic UC symptoms	721:776	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	11	75	theme	colitis	1536:1542	arg1	symptoms					1544:1551	colitis symptoms	1536:1551	colitis symptoms	1536:1551	RESULTS Corylin significantly alleviated colitis symptoms and inhibited inflammatory response in the colon and brain of DSS-induced chronic UC mice.
36634380	5	76	theme	UC	680:681	arg1	model					689:693	chronic UC mouse model	672:693	chronic UC mouse model	672:693	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	4	77	theme	chronic	615:621	arg1	UC					623:624	chronic UC	615:624	chronic UC	615:624	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	13	78	from	expression	1968:1977	arg1	colon					1994:1998	the colon	1990:1998	the colon	1990:1998	In addition, corylin treatment increased the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP), but the expression of 5-HT in the colon was inhibited.
36634380	4	79	theme	corylin	526:532	arg1	mechanism					513:521	mechanism	513:521	mechanism	513:521	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	4	79	theme	corylin	526:532	arg1	effects					501:507	effects	501:507	effects	501:507	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	7	80	theme	molecule	1004:1011	arg1	proteins					895:902	tight junction (TJ) proteins	875:902	tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1)	875:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	7	81	theme	ionized	972:978	arg1	molecule					1004:1011	the ionized calcium-binding adaptor molecule 1	968:1013	the ionized calcium-binding adaptor molecule 1 (Iba1)	968:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	7	81	theme	ionized	972:978	arg1	Iba1					1016:1019	Iba1	1016:1019	Iba1	1016:1019	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	6	82	located	detected	824:831	arg1	brain					850:854	brain	850:854	brain	850:854	The expression of inflammatory cytokines was detected in the colon and brain.
36634380	6	82	located	detected	824:831	arg2	expression					783:792	The expression	779:792	The expression of inflammatory cytokines	779:818	The expression of inflammatory cytokines was detected in the colon and brain.
36634380	6	82	located	detected	824:831	arg1	colon					840:844	colon	840:844	colon	840:844	The expression of inflammatory cytokines was detected in the colon and brain.
36634380	7	83	theme	calcium-binding	980:994	arg1	molecule					1004:1011	the ionized calcium-binding adaptor molecule 1	968:1013	the ionized calcium-binding adaptor molecule 1 (Iba1)	968:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	7	83	theme	calcium-binding	980:994	arg1	Iba1					1016:1019	Iba1	1016:1019	Iba1	1016:1019	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	15	84	theme	intestinal	2282:2291	arg1	microbiota					2293:2302	intestinal microbiota	2282:2302	intestinal microbiota	2282:2302	Moreover, the intake of corylin transformed the diversity and composition of intestinal microbiota, Bacteroides, Escherichia-Shigella, and Turicibacter were decreased but Dubosiella, Enterorhabdus, and Candidatus_Stoquefichus were increased.
36634380	1	85	theme	BACKGROUND	140:149	arg1	colitis					170:176	BACKGROUND Chronic ulcerative colitis	140:176	BACKGROUND Chronic ulcerative colitis (UC)	140:181	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	85	theme	BACKGROUND	140:149	arg1	disease					197:203	a lifelong disease	186:203	a lifelong disease	186:203	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	85	theme	BACKGROUND	140:149	arg1	UC					179:180	UC	179:180	UC	179:180	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	12	86	theme	intestinal	1663:1672	arg1	barrier					1682:1688	intestinal mucosal barrier	1663:1688	intestinal mucosal barrier	1663:1688	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	0	87	from	production	115:124	arg1	colon					133:137	the colon	129:137	the colon	129:137	Corylin ameliorates chronic ulcerative colitis via regulating the gut-brain axis and promoting 5-hydroxytryptophan production in the colon.
36634380	5	88	theme	therapeutic	725:735	arg1	effect					737:742	the therapeutic effect	721:742	the therapeutic effect of corylin on chronic UC symptoms	721:776	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	5	89	from	effect	737:742	arg1	symptoms					769:776	chronic UC symptoms	758:776	chronic UC symptoms	758:776	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	1	90	theme	ulcerative	159:168	arg1	colitis					170:176	BACKGROUND Chronic ulcerative colitis	140:176	BACKGROUND Chronic ulcerative colitis (UC)	140:181	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	90	theme	ulcerative	159:168	arg1	disease					197:203	a lifelong disease	186:203	a lifelong disease	186:203	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	90	theme	ulcerative	159:168	arg1	UC					179:180	UC	179:180	UC	179:180	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	12	91	theme	barrier	1682:1688	arg1	proteins					1651:1658	The TJ proteins	1644:1658	The TJ proteins of intestinal mucosal barrier and BBB	1644:1696	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	14	92	from	reduction	2158:2166	arg1	colon					2198:2202	the colon	2194:2202	the colon	2194:2202	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36634380	7	93	theme	barrier	950:956	arg1	proteins					895:902	tight junction (TJ) proteins	875:902	tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1)	875:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	13	94	theme	neurotransmitters	1857:1873	arg1	expression					1843:1852	the expression	1839:1852	the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP)	1839:1957	In addition, corylin treatment increased the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP), but the expression of 5-HT in the colon was inhibited.
36634380	16	95	theme	inflammation	2592:2603	arg1	interactions					2605:2616	the inflammation interactions	2588:2616	the inflammation interactions across gut-brain axis	2588:2638	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	13	96	theme	corylin	1811:1817	arg1	treatment					1819:1827	corylin treatment	1811:1827	corylin treatment	1811:1827	In addition, corylin treatment increased the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP), but the expression of 5-HT in the colon was inhibited.
36634380	4	97	dep	interactions	554:565	arg1	the					537:539	the	537:539	the	537:539	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	7	98	theme	mucosal	918:924	arg1	BBB					959:961	BBB	959:961	BBB	959:961	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	7	98	theme	mucosal	918:924	arg1	barrier					926:932	intestinal mucosal barrier	907:932	intestinal mucosal barrier	907:932	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	13	99	from	expression	1843:1852	arg1	brain					1882:1886	the brain	1878:1886	the brain	1878:1886	In addition, corylin treatment increased the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP), but the expression of 5-HT in the colon was inhibited.
36634380	13	99	from	expression	1843:1852	arg1	5-hydroxytryptamine					1900:1918	5-hydroxytryptamine	1900:1918	5-hydroxytryptamine (5-HT)	1900:1925	In addition, corylin treatment increased the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP), but the expression of 5-HT in the colon was inhibited.
36634380	13	99	from	expression	1843:1852	arg1	5-hydroxytryptophan					1931:1949	5-hydroxytryptophan	1931:1949	5-hydroxytryptophan (5-HTP)	1931:1957	In addition, corylin treatment increased the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP), but the expression of 5-HT in the colon was inhibited.
36634380	7	100	from	expression	861:870	arg1	hippocampus					1029:1039	the hippocampus	1025:1039	the hippocampus	1025:1039	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	12	101	from	expression	1720:1729	arg1	hippocampus					1746:1756	the hippocampus	1742:1756	the hippocampus	1742:1756	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	3	102	theme	PURPOSE	376:382	arg1	compound					422:429	a representative flavonoid compound	395:429	a representative flavonoid compound isolated from the Psoraleae Fructus	395:465	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	3	102	theme	PURPOSE	376:382	arg1	Corylin					384:390	PURPOSE Corylin	376:390	PURPOSE Corylin	376:390	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	4	103	theme	inflammation	541:552	arg1	interactions					554:565	inflammation interactions	541:565	inflammation interactions	541:565	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	2	104	theme	bowel	360:364	arg1	diseases					366:373	inflammatory bowel diseases	347:373	inflammatory bowel diseases	347:373	The increasing interest in the role of gut-brain axis is seen in inflammatory bowel diseases.
36634380	0	105	theme	ulcerative	28:37	arg1	colitis					39:45	chronic ulcerative colitis	20:45	chronic ulcerative colitis	20:45	Corylin ameliorates chronic ulcerative colitis via regulating the gut-brain axis and promoting 5-hydroxytryptophan production in the colon.
36634380	1	106	theme	disorders	271:279	arg1	prevalence					243:252	a high prevalence	236:252	a high prevalence of common mental disorders	236:279	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	1	107	with	patients	206:213	arg1	UC					228:229	chronic UC	220:229	chronic UC	220:229	BACKGROUND Chronic ulcerative colitis (UC) is a lifelong disease, patients with chronic UC have a high prevalence of common mental disorders.
36634380	10	108	theme	microbiota	1434:1443	arg1	composition					1445:1455	gut microbiota composition	1430:1455	gut microbiota composition	1430:1455	Finally, the changes of gut microbiota composition were analyzed by 16S rRNA sequencing.
36634380	14	109	theme	5-HTP	2172:2176	arg1	accumulation					2178:2189	5-HTP accumulation	2172:2189	5-HTP accumulation	2172:2189	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36634380	16	110	theme	CONCLUSION	2447:2456	arg1	administration					2466:2479	CONCLUSION Corylin administration	2447:2479	CONCLUSION Corylin administration	2447:2479	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	5	111	dep	METHODS	627:633	arg1	DSS					659:661	DSS	659:661	DSS	659:661	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	5	111	dep	METHODS	627:633	arg1	sodium					651:656	Dextran sulfate sodium	635:656	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model	627:693	METHODS Dextran sulfate sodium (DSS) induced chronic UC mouse model was established to assess the therapeutic effect of corylin on chronic UC symptoms.
36634380	15	112	theme	corylin	2229:2235	arg1	intake					2219:2224	the intake	2215:2224	the intake of corylin transformed the diversity and composition of intestinal microbiota, Bacteroides, Escherichia-Shigella, and Turicibacter	2215:2355	Moreover, the intake of corylin transformed the diversity and composition of intestinal microbiota, Bacteroides, Escherichia-Shigella, and Turicibacter were decreased but Dubosiella, Enterorhabdus, and Candidatus_Stoquefichus were increased.
36634380	3	113	theme	Psoraleae	449:457	arg1	Fructus					459:465	the Psoraleae Fructus	445:465	the Psoraleae Fructus	445:465	PURPOSE Corylin is a representative flavonoid compound isolated from the Psoraleae Fructus.
36634380	7	114	theme	immunofluorescence	1081:1098	arg1	staining					1100:1107	immunofluorescence staining	1081:1107	immunofluorescence staining	1081:1107	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	4	115	dep	effects	501:507	arg1	the					497:499	the	497:499	the	497:499	This study aimed to identify the effects and mechanism of corylin on the inflammation interactions and 5-HT synthesis between the gut and brain in chronic UC.
36634380	15	116	dep	diversity	2253:2261	arg1	the					2249:2251	the	2249:2251	the	2249:2251	Moreover, the intake of corylin transformed the diversity and composition of intestinal microbiota, Bacteroides, Escherichia-Shigella, and Turicibacter were decreased but Dubosiella, Enterorhabdus, and Candidatus_Stoquefichus were increased.
36634380	13	117	theme	5-HT	1982:1985	arg1	expression					1968:1977	the expression	1964:1977	the expression of 5-HT in the colon	1964:1998	In addition, corylin treatment increased the expression of neurotransmitters in the brain, especially 5-hydroxytryptamine (5-HT) and 5-hydroxytryptophan (5-HTP), but the expression of 5-HT in the colon was inhibited.
36634380	2	118	theme	gut-brain	321:329	arg1	axis					331:334	gut-brain axis	321:334	gut-brain axis	321:334	The increasing interest in the role of gut-brain axis is seen in inflammatory bowel diseases.
36634380	7	119	theme	junction	881:888	arg1	proteins					895:902	tight junction (TJ) proteins	875:902	tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1)	875:1020	The expression of tight junction (TJ) proteins of intestinal mucosal barrier and blood-brain barrier (BBB) and the ionized calcium-binding adaptor molecule 1 (Iba1) in the hippocampus were determined by western blotting and immunofluorescence staining.
36634380	16	120	theme	DSS-induced	2493:2503	arg1	colitis					2505:2511	DSS-induced colitis	2493:2511	DSS-induced colitis	2493:2511	CONCLUSION Corylin administration ameliorated DSS-induced colitis and inhibited intestinal inflammation and neuroinflammation via regulating the inflammation interactions across gut-brain axis and increasing 5-HTP generation in the colon.
36634380	12	121	theme	corylin	1779:1785	arg1	treatment					1787:1795	corylin treatment	1779:1795	corylin treatment	1779:1795	The TJ proteins of intestinal mucosal barrier and BBB were improved and the expression of Iba1 in the hippocampus was normalized after corylin treatment.
36634380	6	122	theme	inflammatory	797:808	arg1	cytokines					810:818	inflammatory cytokines	797:818	inflammatory cytokines	797:818	The expression of inflammatory cytokines was detected in the colon and brain.
36634380	14	123	theme	5-HT	2153:2156	arg1	reduction					2158:2166	5-HT reduction	2153:2166	5-HT reduction	2153:2166	Further study firstly proved that corylin could bind to the 5-HTDPC, and then inhibit the expression of 5-HTDPC and VB6, resulting in the 5-HT reduction and 5-HTP accumulation in the colon.
36337617	8	0	theme	complete	1643:1650	arg1	de-acetylation					1652:1665	the complete de-acetylation	1639:1665	the complete de-acetylation	1639:1665	Besides, the complete de-acetylation resulted in a remarkable reduction of immune response.
36337617	5	1	dep	[2,2'-azino-bis	1039:1053	arg1	abilities					1144:1152	radical scavenging abilities	1125:1152	radical scavenging abilities	1125:1152	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	2	2	theme	structure-bioactivity	316:336	arg1	relationship					338:349	the structure-bioactivity relationship	312:349	the structure-bioactivity relationship of okra pectic-polysaccharides (OPP)	312:386	However, the knowledge regarding the structure-bioactivity relationship of okra pectic-polysaccharides (OPP) is still limited.
36337617	4	3	theme	weight	837:842	arg1	distribution					844:855	molecular weight distribution	827:855	molecular weight distribution	827:855	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	3	theme	weight	837:842	arg1	characteristics					810:824	molecular characteristics	800:824	molecular characteristics (molecular weight distribution, particle size, and rheological property)	800:897	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	9	4	theme	theoretical	1871:1881	arg1	foundations					1883:1893	theoretical foundations	1871:1893	theoretical foundations for developing OPP as functional foods or health products	1871:1951	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	1	5	theme	components	201:210	arg1	one					166:168	one	166:168	one	166:168	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	1	5	theme	components	201:210	arg1	components					201:210	the most abundant bioactive components	173:210	the most abundant bioactive components in okra	173:218	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	1	5	theme	components	201:210	arg1	Pectic-polysaccharides					125:146	Pectic-polysaccharides	125:146	Pectic-polysaccharides	125:146	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	2	6	theme	pectic-polysaccharides	359:380	arg1	relationship					338:349	the structure-bioactivity relationship	312:349	the structure-bioactivity relationship of okra pectic-polysaccharides (OPP)	312:386	However, the knowledge regarding the structure-bioactivity relationship of okra pectic-polysaccharides (OPP) is still limited.
36337617	7	7	theme	in	1595:1596	arg1	effect					1622:1627	its in vitro immunostimulatory effect	1591:1627	its in vitro immunostimulatory effect	1591:1627	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	4	8	theme	low	619:621	arg1	values					634:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	8	9	theme	immune	1705:1710	arg1	response					1712:1719	immune response	1705:1719	immune response	1705:1719	Besides, the complete de-acetylation resulted in a remarkable reduction of immune response.
36337617	4	10	dep	characteristics	810:824	arg1	distribution					844:855	molecular weight distribution	827:855	molecular weight distribution	827:855	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	10	dep	characteristics	810:824	arg1	size					867:870	particle size	858:870	particle size	858:870	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	10	dep	characteristics	810:824	arg1	characteristics					810:824	molecular characteristics	800:824	molecular characteristics (molecular weight distribution, particle size, and rheological property)	800:897	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	10	dep	characteristics	810:824	arg1	property					889:896	rheological property	877:896	rheological property	877:896	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	9	11	theme	functional	1917:1926	arg1	OPP					1910:1912	OPP	1910:1912	OPP	1910:1912	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	9	11	theme	functional	1917:1926	arg1	foods					1928:1932	functional foods	1917:1932	functional foods	1917:1932	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	1	12	theme	various	235:241	arg1	effects					270:276	various promising health-promoting effects	235:276	various promising health-promoting effects	235:276	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	4	13	theme	alkaline	676:683	arg1	de-esterification					685:701	mild alkaline de-esterification	671:701	mild alkaline de-esterification	671:701	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	5	14	theme	3-ethylbenzthiazoline-6-sulphonic	1056:1088	arg1	[2,2'-azino-bis					1039:1053	antioxidant activities [2,2'-azino-bis	1016:1053	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)	1016:1101	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	14	theme	3-ethylbenzthiazoline-6-sulphonic	1056:1088	arg1	acid					1090:1093	3-ethylbenzthiazoline-6-sulphonic acid	1056:1093	3-ethylbenzthiazoline-6-sulphonic acid	1056:1093	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	7	15	theme	suitable	1542:1549	arg1	degree					1551:1556	a suitable degree	1540:1556	a suitable degree of acetylation	1540:1571	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	7	15	theme	suitable	1542:1549	arg1	beneficial					1577:1586	beneficial	1577:1586	beneficial	1577:1586	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	6	16	theme	OPP	1347:1349	arg1	activity					1335:1342	antioxidant activity	1323:1342	antioxidant activity of OPP	1323:1349	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	6	17	theme	activity	1335:1342	arg1	decrease					1311:1318	the slight decrease	1300:1318	the slight decrease of antioxidant activity of OPP after the alkaline de-esterification	1300:1386	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	5	18	theme	radical	1125:1131	arg1	abilities					1144:1152	radical scavenging abilities	1125:1152	radical scavenging abilities	1125:1152	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	1	19	theme	abundant	182:189	arg1	components					201:210	the most abundant bioactive components	173:210	the most abundant bioactive components in okra	173:218	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	0	20	from	Effects	0:6	arg1	activities					82:91	antioxidant and immunostimulatory activities	48:91	antioxidant and immunostimulatory activities of okra pectic-polysaccharides	48:122	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	0	21	theme	okra	96:99	arg1	pectic-polysaccharides					101:122	okra pectic-polysaccharides	96:122	okra pectic-polysaccharides	96:122	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	5	22	theme	notable	958:964	arg1	decrease					966:973	the notable decrease	954:973	the notable decrease of DE value	954:985	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	6	23	theme	slight	1304:1309	arg1	decrease					1311:1318	the slight decrease	1300:1318	the slight decrease of antioxidant activity of OPP after the alkaline de-esterification	1300:1386	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	3	24	from	effects	421:427	arg1	antioxidant					484:494	in vitro antioxidant	475:494	in vitro antioxidant	475:494	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	3	24	from	effects	421:427	arg1	activities					518:527	immunostimulatory activities	500:527	immunostimulatory activities of OPP	500:534	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	5	25	theme	ferric	1165:1170	arg1	FRAP					1200:1203	FRAP	1200:1203	FRAP	1200:1203	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	25	theme	ferric	1165:1170	arg1	power					1193:1197	ferric reducing antioxidant power	1165:1197	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP	1016:1212	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	4	26	theme	high	583:586	arg1	values					634:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	27	theme	chemical	722:729	arg1	linkage					787:793	glycosidic linkage	776:793	glycosidic linkage	776:793	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	27	theme	chemical	722:729	arg1	structures					731:740	their primary chemical structures	708:740	their primary chemical structures (compositional monosaccharide and glycosidic linkage)	708:794	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	27	theme	chemical	722:729	arg1	monosaccharide					757:770	compositional monosaccharide	743:770	compositional monosaccharide	743:770	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	5	28	theme	antioxidant	1269:1279	arg1	activity					1281:1288	its antioxidant activity	1265:1288	its antioxidant activity	1265:1288	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	0	29	theme	various	11:17	arg1	degrees					19:25	various degrees	11:25	various degrees of esterification	11:43	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	5	30	theme	antioxidant	1181:1191	arg1	FRAP					1200:1203	FRAP	1200:1203	FRAP	1200:1203	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	30	theme	antioxidant	1181:1191	arg1	power					1193:1197	ferric reducing antioxidant power	1165:1197	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP	1016:1212	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	9	31	theme	DE	1788:1789	arg1	value					1791:1795	DE value	1788:1795	DE value	1788:1795	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	4	32	theme	molecular	800:808	arg1	distribution					844:855	molecular weight distribution	827:855	molecular weight distribution	827:855	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	32	theme	molecular	800:808	arg1	size					867:870	particle size	858:870	particle size	858:870	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	32	theme	molecular	800:808	arg1	characteristics					810:824	molecular characteristics	800:824	molecular characteristics (molecular weight distribution, particle size, and rheological property)	800:897	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	32	theme	molecular	800:808	arg1	property					889:896	rheological property	877:896	rheological property	877:896	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	3	33	theme	in	475:476	arg1	antioxidant					484:494	in vitro antioxidant	475:494	in vitro antioxidant	475:494	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	9	34	theme	value	1791:1795	arg1	effect					1778:1783	the effect	1774:1783	the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products	1774:1951	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	3	35	theme	various	432:438	arg1	degrees					440:446	various degrees	432:446	various degrees of esterification (DEs)	432:470	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	7	36	theme	immunostimulatory	1473:1489	arg1	related					1517:1523	related	1517:1523	related	1517:1523	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	7	36	theme	immunostimulatory	1473:1489	arg1	effect					1491:1496	the immunostimulatory effect	1469:1496	the immunostimulatory effect of OPP	1469:1503	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	5	37	theme	activities	1028:1037	arg1	[2,2'-azino-bis					1039:1053	antioxidant activities [2,2'-azino-bis	1016:1053	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)	1016:1101	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	37	theme	activities	1028:1037	arg1	acid					1090:1093	3-ethylbenzthiazoline-6-sulphonic acid	1056:1093	3-ethylbenzthiazoline-6-sulphonic acid	1056:1093	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	37	theme	activities	1028:1037	arg1	ABTS					1097:1100	ABTS	1097:1100	ABTS	1097:1100	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	1	38	contain	possess	227:233	arg1	Pectic-polysaccharides					125:146	Pectic-polysaccharides	125:146	Pectic-polysaccharides	125:146	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	1	38	contain	possess	227:233	arg1	one					166:168	one	166:168	one	166:168	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	1	38	contain	possess	227:233	arg1	components					201:210	the most abundant bioactive components	173:210	the most abundant bioactive components in okra	173:218	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	1	38	contain	possess	227:233	arg2	effects					270:276	various promising health-promoting effects	235:276	various promising health-promoting effects	235:276	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	4	39	theme	glycosidic	776:785	arg1	linkage					787:793	glycosidic linkage	776:793	glycosidic linkage	776:793	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	39	theme	glycosidic	776:785	arg1	structures					731:740	their primary chemical structures	708:740	their primary chemical structures (compositional monosaccharide and glycosidic linkage)	708:794	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	3	40	theme	immunostimulatory	500:516	arg1	activities					518:527	immunostimulatory activities	500:527	immunostimulatory activities of OPP	500:534	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	1	41	theme	promising	243:251	arg1	effects					270:276	various promising health-promoting effects	235:276	various promising health-promoting effects	235:276	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	5	42	theme	value	981:985	arg1	decrease					966:973	the notable decrease	954:973	the notable decrease of DE value	954:985	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	6	43	theme	acid	1441:1444	arg1	content					1446:1452	uronic acid content	1434:1452	uronic acid content	1434:1452	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	6	44	theme	alkaline	1361:1368	arg1	de-esterification					1370:1386	the alkaline de-esterification	1357:1386	the alkaline de-esterification	1357:1386	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	9	45	theme	OPP	1847:1849	arg1	activities					1833:1842	antioxidant and immunomodulatory activities	1800:1842	antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products	1800:1951	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	4	46	theme	particle	858:865	arg1	size					867:870	particle size	858:870	particle size	858:870	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	46	theme	particle	858:865	arg1	characteristics					810:824	molecular characteristics	800:824	molecular characteristics (molecular weight distribution, particle size, and rheological property)	800:897	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	47	dep	low	619:621	arg1	%					628:628	4.77%	624:628	4.77%	624:628	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	6	48	theme	slight	1415:1420	arg1	decrease					1422:1429	the slight decrease	1411:1429	the slight decrease of uronic acid content	1411:1452	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	9	49	from	effect	1778:1783	arg1	activities					1833:1842	antioxidant and immunomodulatory activities	1800:1842	antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products	1800:1951	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	4	50	theme	middle	598:603	arg1	values					634:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	1	51	theme	bioactive	191:199	arg1	components					201:210	the most abundant bioactive components	173:210	the most abundant bioactive components in okra	173:218	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	9	52	theme	antioxidant	1800:1810	arg1	activities					1833:1842	antioxidant and immunomodulatory activities	1800:1842	antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products	1800:1951	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	4	53	theme	molecular	827:835	arg1	distribution					844:855	molecular weight distribution	827:855	molecular weight distribution	827:855	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	53	theme	molecular	827:835	arg1	characteristics					810:824	molecular characteristics	800:824	molecular characteristics (molecular weight distribution, particle size, and rheological property)	800:897	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	54	theme	DE	631:632	arg1	values					634:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	high (42.13%), middle (25.88%), and low (4.77%) DE values	583:639	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	2	55	theme	okra	354:357	arg1	OPP					383:385	OPP	383:385	OPP	383:385	However, the knowledge regarding the structure-bioactivity relationship of okra pectic-polysaccharides (OPP) is still limited.
36337617	2	55	theme	okra	354:357	arg1	pectic-polysaccharides					359:380	okra pectic-polysaccharides	354:380	okra pectic-polysaccharides (OPP)	354:386	However, the knowledge regarding the structure-bioactivity relationship of okra pectic-polysaccharides (OPP) is still limited.
36337617	8	56	theme	remarkable	1681:1690	arg1	reduction					1692:1700	a remarkable reduction	1679:1700	a remarkable reduction of immune response	1679:1719	Besides, the complete de-acetylation resulted in a remarkable reduction of immune response.
36337617	4	57	dep	structures	731:740	arg1	linkage					787:793	glycosidic linkage	776:793	glycosidic linkage	776:793	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	57	dep	structures	731:740	arg1	structures					731:740	their primary chemical structures	708:740	their primary chemical structures (compositional monosaccharide and glycosidic linkage)	708:794	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	57	dep	structures	731:740	arg1	monosaccharide					757:770	compositional monosaccharide	743:770	compositional monosaccharide	743:770	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	58	dep	middle	598:603	arg1	%					611:611	25.88%	606:611	25.88%	606:611	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	7	59	theme	acetylation	1561:1571	arg1	degree					1551:1556	a suitable degree	1540:1556	a suitable degree of acetylation	1540:1571	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	7	59	theme	acetylation	1561:1571	arg1	beneficial					1577:1586	beneficial	1577:1586	beneficial	1577:1586	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	6	60	theme	antioxidant	1323:1333	arg1	activity					1335:1342	antioxidant activity	1323:1342	antioxidant activity of OPP	1323:1349	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	8	61	theme	response	1712:1719	arg1	reduction					1692:1700	a remarkable reduction	1679:1700	a remarkable reduction of immune response	1679:1719	Besides, the complete de-acetylation resulted in a remarkable reduction of immune response.
36337617	5	62	theme	scavenging	1133:1142	arg1	abilities					1144:1152	radical scavenging abilities	1125:1152	radical scavenging abilities	1125:1152	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	4	63	theme	rheological	877:887	arg1	characteristics					810:824	molecular characteristics	800:824	molecular characteristics (molecular weight distribution, particle size, and rheological property)	800:897	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	63	theme	rheological	877:887	arg1	property					889:896	rheological property	877:896	rheological property	877:896	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	9	64	theme	health	1937:1942	arg1	OPP					1910:1912	OPP	1910:1912	OPP	1910:1912	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	9	64	theme	health	1937:1942	arg1	products					1944:1951	health products	1937:1951	health products	1937:1951	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	4	65	theme	mild	671:674	arg1	de-esterification					685:701	mild alkaline de-esterification	671:701	mild alkaline de-esterification	671:701	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	3	66	dep	in	475:476	arg1	vitro					478:482	vitro	478:482	vitro	478:482	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	0	67	theme	immunostimulatory	64:80	arg1	activities					82:91	antioxidant and immunostimulatory activities	48:91	antioxidant and immunostimulatory activities of okra pectic-polysaccharides	48:122	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	1	68	from	components	201:210	arg1	okra					215:218	okra	215:218	okra	215:218	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	1	69	theme	most	177:180	arg1	components					201:210	the most abundant bioactive components	173:210	the most abundant bioactive components in okra	173:218	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	0	70	theme	pectic-polysaccharides	101:122	arg1	activities					82:91	antioxidant and immunostimulatory activities	48:91	antioxidant and immunostimulatory activities of okra pectic-polysaccharides	48:122	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	7	71	dep	in	1595:1596	arg1	vitro					1598:1602	vitro	1598:1602	vitro	1598:1602	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	5	72	theme	nitric	1107:1112	arg1	oxide					1114:1118	nitric oxide	1107:1118	nitric oxide (NO)	1107:1123	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	72	theme	nitric	1107:1112	arg1	NO					1121:1122	NO	1121:1122	NO	1121:1122	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	4	73	theme	compositional	743:755	arg1	structures					731:740	their primary chemical structures	708:740	their primary chemical structures (compositional monosaccharide and glycosidic linkage)	708:794	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	73	theme	compositional	743:755	arg1	monosaccharide					757:770	compositional monosaccharide	743:770	compositional monosaccharide	743:770	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	74	dep	high	583:586	arg1	%					594:594	42.13%	589:594	42.13%	589:594	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	0	75	theme	degrees	19:25	arg1	Effects					0:6	Effects	0:6	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.	0:123	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	5	76	theme	reducing	1172:1179	arg1	FRAP					1200:1203	FRAP	1200:1203	FRAP	1200:1203	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	76	theme	reducing	1172:1179	arg1	power					1193:1197	ferric reducing antioxidant power	1165:1197	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP	1016:1212	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	4	77	theme	primary	714:720	arg1	linkage					787:793	glycosidic linkage	776:793	glycosidic linkage	776:793	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	77	theme	primary	714:720	arg1	structures					731:740	their primary chemical structures	708:740	their primary chemical structures (compositional monosaccharide and glycosidic linkage)	708:794	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	4	77	theme	primary	714:720	arg1	monosaccharide					757:770	compositional monosaccharide	743:770	compositional monosaccharide	743:770	Results displayed that OPP with high (42.13%), middle (25.88%), and low (4.77%) DE values were successfully prepared by mild alkaline de-esterification, and their primary chemical structures (compositional monosaccharide and glycosidic linkage) and molecular characteristics (molecular weight distribution, particle size, and rheological property) were overall stable.
36337617	0	78	theme	esterification	30:43	arg1	degrees					19:25	various degrees	11:25	various degrees of esterification	11:43	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	7	79	theme	immunostimulatory	1604:1620	arg1	effect					1622:1627	its in vitro immunostimulatory effect	1591:1627	its in vitro immunostimulatory effect	1591:1627	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	3	80	theme	esterification	451:464	arg1	degrees					440:446	various degrees	432:446	various degrees of esterification (DEs)	432:470	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	1	81	theme	health-promoting	253:268	arg1	effects					270:276	various promising health-promoting effects	235:276	various promising health-promoting effects	235:276	Pectic-polysaccharides are considered as one of the most abundant bioactive components in okra, which possess various promising health-promoting effects.
36337617	0	82	theme	antioxidant	48:58	arg1	activities					82:91	antioxidant and immunostimulatory activities	48:91	antioxidant and immunostimulatory activities of okra pectic-polysaccharides	48:122	Effects of various degrees of esterification on antioxidant and immunostimulatory activities of okra pectic-polysaccharides.
36337617	7	83	theme	OPP	1501:1503	arg1	related					1517:1523	related	1517:1523	related	1517:1523	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	7	83	theme	OPP	1501:1503	arg1	effect					1491:1496	the immunostimulatory effect	1469:1496	the immunostimulatory effect of OPP	1469:1503	Nevertheless, the immunostimulatory effect of OPP was closely related to its DE, and a suitable degree of acetylation was beneficial to its in vitro immunostimulatory effect.
36337617	5	84	theme	antioxidant	1016:1026	arg1	activities					1028:1037	antioxidant activities	1016:1037	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)	1016:1101	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	3	85	theme	degrees	440:446	arg1	effects					421:427	effects	421:427	effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP	421:534	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	3	86	theme	OPP	532:534	arg1	antioxidant					484:494	in vitro antioxidant	475:494	in vitro antioxidant	475:494	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	3	86	theme	OPP	532:534	arg1	activities					518:527	immunostimulatory activities	500:527	immunostimulatory activities of OPP	500:534	In this study, effects of various degrees of esterification (DEs) on in vitro antioxidant and immunostimulatory activities of OPP were analyzed.
36337617	5	87	theme	DE	978:979	arg1	value					981:985	DE value	978:985	DE value	978:985	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	9	88	theme	immunomodulatory	1816:1831	arg1	activities					1833:1842	antioxidant and immunomodulatory activities	1800:1842	antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products	1800:1951	The findings are beneficial to better understanding the effect of DE value on antioxidant and immunomodulatory activities of OPP, which also provide theoretical foundations for developing OPP as functional foods or health products.
36337617	6	89	theme	content	1446:1452	arg1	decrease					1422:1429	the slight decrease	1411:1429	the slight decrease of uronic acid content	1411:1452	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
36337617	5	90	theme	OPP	1210:1212	arg1	[2,2'-azino-bis					1039:1053	antioxidant activities [2,2'-azino-bis	1016:1053	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)	1016:1101	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	90	theme	OPP	1210:1212	arg1	acid					1090:1093	3-ethylbenzthiazoline-6-sulphonic acid	1056:1093	3-ethylbenzthiazoline-6-sulphonic acid	1056:1093	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	90	theme	OPP	1210:1212	arg1	power					1193:1197	ferric reducing antioxidant power	1165:1197	antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP	1016:1212	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	90	theme	OPP	1210:1212	arg1	oxide					1114:1118	nitric oxide	1107:1118	nitric oxide (NO)	1107:1123	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	90	theme	OPP	1210:1212	arg1	ABTS					1097:1100	ABTS	1097:1100	ABTS	1097:1100	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	90	theme	OPP	1210:1212	arg1	FRAP					1200:1203	FRAP	1200:1203	FRAP	1200:1203	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	5	90	theme	OPP	1210:1212	arg1	NO					1121:1122	NO	1121:1122	NO	1121:1122	Additionally, results showed that the notable decrease of DE value did not significantly affect antioxidant activities [2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and nitric oxide (NO) radical scavenging abilities as well as ferric reducing antioxidant power (FRAP)] of OPP, suggesting that the DE was not closely related to its antioxidant activity.
36337617	6	91	theme	uronic	1434:1439	arg1	content					1446:1452	uronic acid content	1434:1452	uronic acid content	1434:1452	In fact, the slight decrease of antioxidant activity of OPP after the alkaline de-esterification might be attributed to the slight decrease of uronic acid content.
35182669	2	0	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	medicine					248:255	traditional Chinese medicine	228:255	traditional Chinese medicine	228:255	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	0	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	agents					217:222	flavoring agents	207:222	flavoring agents	207:222	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	0	theme	ETHNOPHARMACOLOGICAL	124:143	arg1	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	8	1	theme	tissue	1012:1017	arg1	damage					1032:1037	LPS-induced lung tissue pathological damage	995:1037	LPS-induced lung tissue pathological damage	995:1037	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	9	2	theme	lavage	1163:1168	arg1	fluid					1170:1174	the bronchoalveolar lavage fluid	1143:1174	the bronchoalveolar lavage fluid of mice with LPS-induced ALI	1143:1203	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	10	3	theme	malondialdehyde	1247:1261	arg1	levels					1237:1242	the levels	1233:1242	the levels of malondialdehyde, TNF-α, IL-6, and IL-1β	1233:1285	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	8	4	theme	lung	1007:1010	arg1	damage					1032:1037	LPS-induced lung tissue pathological damage	995:1037	LPS-induced lung tissue pathological damage	995:1037	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	7	5	dep	in	866:867	arg1	vivo					869:872	vivo	869:872	vivo	869:872	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	10	6	theme	serum	1392:1396	arg1	samples					1398:1404	serum samples	1392:1404	serum samples	1392:1404	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	11	7	theme	related	1461:1467	arg1	proteins					1452:1459	proteins	1452:1459	proteins related to the TLR4/Myd88/NF-κB pathway	1452:1499	We also found that these three AEOs affected proteins related to the TLR4/Myd88/NF-κB pathway.
35182669	3	8	theme	herbal	379:384	arg1	medicines					386:394	Chinese herbal medicines	371:394	Chinese herbal medicines	371:394	AIM OF THE STUDY This study aims to investigate the protective effects of Chinese herbal medicines, including six Amomum Roxb.
35182669	10	9	theme	alveolar	1365:1372	arg1	fluid					1381:1385	alveolar lavage fluid	1365:1385	alveolar lavage fluid	1365:1385	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	4	10	from	lipopolysaccharide	490:507	arg1	mice					518:521	mice	518:521	mice	518:521	essential oils (AEOs), against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.
35182669	9	11	theme	mice	1179:1182	arg1	fluid					1170:1174	the bronchoalveolar lavage fluid	1143:1174	the bronchoalveolar lavage fluid of mice with LPS-induced ALI	1143:1203	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	7	12	theme	acute	883:887	arg1	injury					894:899	acute lung injury	883:899	acute lung injury	883:899	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	3	13	theme	medicines	386:394	arg1	effects					360:366	the protective effects	345:366	the protective effects of Chinese herbal medicines	345:394	AIM OF THE STUDY This study aims to investigate the protective effects of Chinese herbal medicines, including six Amomum Roxb.
35182669	8	14	theme	pathological	1019:1030	arg1	damage					1032:1037	LPS-induced lung tissue pathological damage	995:1037	LPS-induced lung tissue pathological damage	995:1037	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	5	15	theme	gas	591:593	arg1	chromatography					595:608	gas chromatography	591:608	gas chromatography	591:608	MATERIALS AND METHODS The compositions of AEOs were analyzed using gas chromatography - mass spectrometry.
35182669	1	16	theme	lipopolysaccharide-induced	52:77	arg1	injury					90:95	lipopolysaccharide-induced acute lung injury	52:95	lipopolysaccharide-induced acute lung injury	52:95	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics.
35182669	7	17	theme	LPS	834:836	arg1	instillation					818:829	intratracheal instillation	804:829	intratracheal instillation of LPS	804:836	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	7	18	theme	C57	708:710	arg1	mice					712:715	C57 mice	708:715	C57 mice	708:715	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	4	19	theme	essential	424:432	arg1	AEOs					440:443	AEOs	440:443	AEOs	440:443	essential oils (AEOs), against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.
35182669	4	19	theme	essential	424:432	arg1	oils					434:437	essential oils	424:437	essential oils (AEOs)	424:444	essential oils (AEOs), against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.
35182669	1	20	theme	acute	79:83	arg1	injury					90:95	lipopolysaccharide-induced acute lung injury	52:95	lipopolysaccharide-induced acute lung injury	52:95	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics.
35182669	1	21	from	oils	44:47	arg1	injury					90:95	lipopolysaccharide-induced acute lung injury	52:95	lipopolysaccharide-induced acute lung injury	52:95	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics.
35182669	10	22	from	levels	1305:1310	arg1	fluid					1381:1385	alveolar lavage fluid	1365:1385	alveolar lavage fluid	1365:1385	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	10	22	from	levels	1305:1310	arg1	samples					1398:1404	serum samples	1392:1404	serum samples	1392:1404	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	10	22	from	levels	1305:1310	arg1	tissue					1357:1362	lung tissue	1352:1362	lung tissue	1352:1362	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	3	23	theme	Chinese	371:377	arg1	medicines					386:394	Chinese herbal medicines	371:394	Chinese herbal medicines	371:394	AIM OF THE STUDY This study aims to investigate the protective effects of Chinese herbal medicines, including six Amomum Roxb.
35182669	2	24	theme	Amomum	163:168	arg1	medicine					248:255	traditional Chinese medicine	228:255	traditional Chinese medicine	228:255	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	24	theme	Amomum	163:168	arg1	agents					217:222	flavoring agents	207:222	flavoring agents	207:222	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	24	theme	Amomum	163:168	arg1	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	5	25	theme	mass	612:615	arg1	spectrometry					617:628	mass spectrometry	612:628	mass spectrometry	612:628	MATERIALS AND METHODS The compositions of AEOs were analyzed using gas chromatography - mass spectrometry.
35182669	7	26	theme	injury	894:899	arg1	model					874:878	an in vivo model	863:878	an in vivo model of acute lung injury	863:899	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	1	27	theme	lung	85:88	arg1	injury					90:95	lipopolysaccharide-induced acute lung injury	52:95	lipopolysaccharide-induced acute lung injury	52:95	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics.
35182669	5	28	dep	MATERIALS	524:532	arg1	compositions					550:561	The compositions	546:561	MATERIALS AND METHODS The compositions of AEOs	524:569	MATERIALS AND METHODS The compositions of AEOs were analyzed using gas chromatography - mass spectrometry.
35182669	2	29	theme	Several	155:161	arg1	medicine					248:255	traditional Chinese medicine	228:255	traditional Chinese medicine	228:255	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	29	theme	Several	155:161	arg1	agents					217:222	flavoring agents	207:222	flavoring agents	207:222	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	29	theme	Several	155:161	arg1	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	3	30	dep	AIM	297:299	arg1	aims					325:328	aims	325:328	aims	325:328	AIM OF THE STUDY This study aims to investigate the protective effects of Chinese herbal medicines, including six Amomum Roxb.
35182669	10	31	theme	TNF-α	1264:1268	arg1	levels					1237:1242	the levels	1233:1242	the levels of malondialdehyde, TNF-α, IL-6, and IL-1β	1233:1285	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	0	32	theme	Protective	0:9	arg1	effect					11:16	Protective effect	0:16	Protective effect of Amomum Roxb	0:31	Protective effect of Amomum Roxb.
35182669	5	33	theme	AEOs	566:569	arg1	compositions					550:561	The compositions	546:561	MATERIALS AND METHODS The compositions of AEOs	524:569	MATERIALS AND METHODS The compositions of AEOs were analyzed using gas chromatography - mass spectrometry.
35182669	8	34	theme	LPS-induced	995:1005	arg1	damage					1032:1037	LPS-induced lung tissue pathological damage	995:1037	LPS-induced lung tissue pathological damage	995:1037	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	0	35	theme	Amomum	21:26	arg1	Roxb					28:31	Amomum Roxb	21:31	Amomum Roxb	21:31	Protective effect of Amomum Roxb.
35182669	1	36	theme	essential	34:42	arg1	oils					44:47	essential oils	34:47	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics	34:121	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics.
35182669	12	37	theme	oxidative	1586:1594	arg1	stress					1596:1601	oxidative stress	1586:1601	oxidative stress	1586:1601	CONCLUSIONS In summary, our findings revealed that AEOs ameliorate inflammatory and oxidative stress in mice with ALI through the TLR4/Myd88/NF-κB pathway.
35182669	7	38	theme	lung	889:892	arg1	injury					894:899	acute lung injury	883:899	acute lung injury	883:899	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	10	39	theme	IL-1β	1281:1285	arg1	levels					1237:1242	the levels	1233:1242	the levels of malondialdehyde, TNF-α, IL-6, and IL-1β	1233:1285	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	7	40	theme	intratracheal	804:816	arg1	instillation					818:829	intratracheal instillation	804:829	intratracheal instillation of LPS	804:836	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	10	41	theme	dismutase	1326:1334	arg1	levels					1305:1310	the levels	1301:1310	the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples	1301:1404	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	11	42	theme	TLR4/Myd88/NF-κB	1476:1491	arg1	pathway					1493:1499	the TLR4/Myd88/NF-κB pathway	1472:1499	the TLR4/Myd88/NF-κB pathway	1472:1499	We also found that these three AEOs affected proteins related to the TLR4/Myd88/NF-κB pathway.
35182669	3	43	theme	STUDY	308:312	arg1	AIM					297:299	AIM	297:299	AIM OF THE STUDY This study aims to investigate the protective effects of Chinese herbal medicines, including six Amomum Roxb.	297:422	AIM OF THE STUDY This study aims to investigate the protective effects of Chinese herbal medicines, including six Amomum Roxb.
35182669	4	44	theme	acute	455:459	arg1	ALI					474:476	ALI	474:476	ALI	474:476	essential oils (AEOs), against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.
35182669	4	44	theme	acute	455:459	arg1	injury					466:471	acute lung injury	455:471	acute lung injury (ALI)	455:477	essential oils (AEOs), against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.
35182669	9	45	theme	wet/dry	1091:1097	arg1	ratios					1106:1111	lung wet/dry weight ratios	1086:1111	lung wet/dry weight ratios	1086:1111	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	1	46	dep	oils	44:47	arg1	mice					97:100	mice	97:100	mice	97:100	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics.
35182669	1	46	dep	oils	44:47	arg1	metabolomics					110:121	its metabolomics	106:121	its metabolomics	106:121	essential oils in lipopolysaccharide-induced acute lung injury mice and its metabolomics.
35182669	7	47	theme	consecutive	776:786	arg1	days					788:791	seven consecutive days	770:791	seven consecutive days	770:791	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	12	48	theme	TLR4/Myd88/NF-κB	1632:1647	arg1	pathway					1649:1655	the TLR4/Myd88/NF-κB pathway	1628:1655	the TLR4/Myd88/NF-κB pathway	1628:1655	CONCLUSIONS In summary, our findings revealed that AEOs ameliorate inflammatory and oxidative stress in mice with ALI through the TLR4/Myd88/NF-κB pathway.
35182669	9	49	theme	weight	1099:1104	arg1	ratios					1106:1111	lung wet/dry weight ratios	1086:1111	lung wet/dry weight ratios	1086:1111	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	0	50	theme	Roxb	28:31	arg1	effect					11:16	Protective effect	0:16	Protective effect of Amomum Roxb	0:31	Protective effect of Amomum Roxb.
35182669	9	51	with	mice	1179:1182	arg1	ALI					1201:1203	LPS-induced ALI	1189:1203	LPS-induced ALI	1189:1203	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	10	52	theme	superoxide	1315:1324	arg1	dismutase					1326:1334	superoxide dismutase	1315:1334	superoxide dismutase	1315:1334	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	4	53	theme	lung	461:464	arg1	ALI					474:476	ALI	474:476	ALI	474:476	essential oils (AEOs), against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.
35182669	4	53	theme	lung	461:464	arg1	injury					466:471	acute lung injury	455:471	acute lung injury (ALI)	455:477	essential oils (AEOs), against acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice.
35182669	2	54	theme	Chinese	240:246	arg1	medicine					248:255	traditional Chinese medicine	228:255	traditional Chinese medicine	228:255	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	54	theme	Chinese	240:246	arg1	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	12	55	dep	CONCLUSIONS	1502:1512	arg1	summary					1517:1523	summary	1517:1523	summary	1517:1523	CONCLUSIONS In summary, our findings revealed that AEOs ameliorate inflammatory and oxidative stress in mice with ALI through the TLR4/Myd88/NF-κB pathway.
35182669	10	56	theme	IL-6	1271:1274	arg1	levels					1237:1242	the levels	1233:1242	the levels of malondialdehyde, TNF-α, IL-6, and IL-1β	1233:1285	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	2	57	theme	traditional	228:238	arg1	medicine					248:255	traditional Chinese medicine	228:255	traditional Chinese medicine	228:255	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	57	theme	traditional	228:238	arg1	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	9	58	theme	protein	1117:1123	arg1	concentrations					1125:1138	protein concentrations	1117:1138	protein concentrations	1117:1138	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	8	59	theme	damage	1032:1037	arg1	amelioration					979:990	amelioration	979:990	amelioration of LPS-induced lung tissue pathological damage	979:1037	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	8	59	theme	damage	1032:1037	arg1	AEOs					930:933	three AEOs	924:933	three AEOs demonstrating anti-inflammatory effects	924:973	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	7	60	theme	atomization	747:757	arg1	system					759:764	atomization system	747:764	atomization system for seven consecutive days	747:791	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	9	61	theme	LPS-induced	1189:1199	arg1	ALI					1201:1203	LPS-induced ALI	1189:1203	LPS-induced ALI	1189:1203	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	8	62	theme	anti-inflammatory	949:965	arg1	effects					967:973	anti-inflammatory effects	949:973	anti-inflammatory effects	949:973	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	2	63	theme	flavoring	207:215	arg1	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	63	theme	flavoring	207:215	arg1	agents					217:222	flavoring agents	207:222	flavoring agents	207:222	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	9	64	theme	lung	1086:1089	arg1	ratios					1106:1111	lung wet/dry weight ratios	1086:1111	lung wet/dry weight ratios	1086:1111	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	7	65	theme	in	866:867	arg1	model					874:878	an in vivo model	863:878	an in vivo model of acute lung injury	863:899	C57 mice received AEOs (100 mg/kg) via atomization system for seven consecutive days, and then, intratracheal instillation of LPS was applied to establish an in vivo model of acute lung injury.
35182669	3	66	theme	protective	349:358	arg1	effects					360:366	the protective effects	345:366	the protective effects of Chinese herbal medicines	345:394	AIM OF THE STUDY This study aims to investigate the protective effects of Chinese herbal medicines, including six Amomum Roxb.
35182669	2	67	theme	inflammation-related	266:285	arg1	diseases					287:294	inflammation-related diseases	266:294	inflammation-related diseases	266:294	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	10	68	theme	catalase	1340:1347	arg1	levels					1305:1310	the levels	1301:1310	the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples	1301:1404	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	8	69	dep	RESULTS	902:908	arg1	identified					913:922	identified	913:922	identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage	913:1037	RESULTS We identified three AEOs demonstrating anti-inflammatory effects and amelioration of LPS-induced lung tissue pathological damage.
35182669	10	70	theme	lung	1352:1355	arg1	tissue					1357:1362	lung tissue	1352:1362	lung tissue	1352:1362	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	10	71	theme	lavage	1374:1379	arg1	fluid					1381:1385	alveolar lavage fluid	1365:1385	alveolar lavage fluid	1365:1385	Additionally, AEOs reduced the levels of malondialdehyde, TNF-α, IL-6, and IL-1β but increased the levels of superoxide dismutase and catalase in lung tissue, alveolar lavage fluid, and serum samples.
35182669	2	72	theme	RELEVANCE	145:153	arg1	medicine					248:255	traditional Chinese medicine	228:255	traditional Chinese medicine	228:255	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	72	theme	RELEVANCE	145:153	arg1	agents					217:222	flavoring agents	207:222	flavoring agents	207:222	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	72	theme	RELEVANCE	145:153	arg1	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	9	73	theme	bronchoalveolar	1147:1161	arg1	fluid					1170:1174	the bronchoalveolar lavage fluid	1143:1174	the bronchoalveolar lavage fluid of mice with LPS-induced ALI	1143:1203	Furthermore, we found that these AEOs reduced lung wet/dry weight ratios and protein concentrations in the bronchoalveolar lavage fluid of mice with LPS-induced ALI.
35182669	12	74	with	mice	1606:1609	arg1	ALI					1616:1618	ALI	1616:1618	ALI	1616:1618	CONCLUSIONS In summary, our findings revealed that AEOs ameliorate inflammatory and oxidative stress in mice with ALI through the TLR4/Myd88/NF-κB pathway.
35182669	2	75	used	used	191:194	arg2	agents					217:222	flavoring agents	207:222	flavoring agents	207:222	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	75	used	used	191:194	arg2	species					170:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species	124:176	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
35182669	2	75	used	used	191:194	arg2	medicine					248:255	traditional Chinese medicine	228:255	traditional Chinese medicine	228:255	ETHNOPHARMACOLOGICAL RELEVANCE Several Amomum species are commonly used in food as flavoring agents and traditional Chinese medicine to treat inflammation-related diseases.
36592529	8	0	theme	antibiotic	1163:1172	arg1	-treatment					1188:1197	antibiotic cocktail (Abx)-treatment	1163:1197	antibiotic cocktail (Abx)-treatment	1163:1197	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	6	1	theme	factors	1036:1042	arg1	secretion					1006:1014	secretion	1006:1014	secretion of pro-inflammatory factors	1006:1042	Results also revealed that macrophages from TRPC HeptaKO mice exhibited M1 polarization and enhanced secretion of pro-inflammatory factors.
36592529	8	2	theme	disease	1268:1274	arg1	severity					1276:1283	disease severity	1268:1283	disease severity	1268:1283	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	5	3	theme	Flow	728:731	arg1	analysis					743:750	Flow cytometry analysis	728:750	Flow cytometry analysis	728:750	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	8	4	theme	HeptaKO	1205:1211	arg1	mice					1213:1216	TRPC HeptaKO mice	1200:1216	TRPC HeptaKO mice	1200:1216	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	2	5	from	consequences	315:326	arg1	mice					359:362	mice	359:362	mice	359:362	Here we explored the consequences of ablating all seven TRPCs in mice focusing on colitis.
36592529	9	6	theme	gut	1439:1441	arg1	disorder					1454:1461	gut microbiota disorder	1439:1461	gut microbiota disorder	1439:1461	Collectively, these data suggest that ablation of all TRPCs promotes the development of DSS-induced colitis by inducing pro-inflammatory macrophages and gut microbiota disorder.
36592529	5	7	from	infiltration	768:779	arg1	propria					835:841	colonic lamina propria	820:841	colonic lamina propria	820:841	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	7	8	theme	TRPC	1130:1133	arg1	mice					1143:1146	DSS-treated TRPC HeptaKO mice	1118:1146	DSS-treated TRPC HeptaKO mice	1118:1146	In addition, the composition of gut microbiota was markedly disturbed in DSS-treated TRPC HeptaKO mice.
36592529	5	9	theme	HeptaKO	891:897	arg1	mice					899:902	DSS-treated TRPC HeptaKO mice	874:902	DSS-treated TRPC HeptaKO mice	874:902	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	9	10	theme	microbiota	1443:1452	arg1	disorder					1454:1461	gut microbiota disorder	1439:1461	gut microbiota disorder	1439:1461	Collectively, these data suggest that ablation of all TRPCs promotes the development of DSS-induced colitis by inducing pro-inflammatory macrophages and gut microbiota disorder.
36592529	8	11	theme	cocktail	1174:1181	arg1	-treatment					1188:1197	antibiotic cocktail (Abx)-treatment	1163:1197	antibiotic cocktail (Abx)-treatment	1163:1197	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	9	12	theme	TRPCs	1340:1344	arg1	ablation					1324:1331	ablation	1324:1331	ablation of all TRPCs	1324:1344	Collectively, these data suggest that ablation of all TRPCs promotes the development of DSS-induced colitis by inducing pro-inflammatory macrophages and gut microbiota disorder.
36592529	7	13	theme	HeptaKO	1135:1141	arg1	mice					1143:1146	DSS-treated TRPC HeptaKO mice	1118:1146	DSS-treated TRPC HeptaKO mice	1118:1146	In addition, the composition of gut microbiota was markedly disturbed in DSS-treated TRPC HeptaKO mice.
36592529	1	14	theme	canonical	138:146	arg1	contributors					215:226	important contributors	205:226	important contributors to calcium fluxes	205:244	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	1	14	theme	canonical	138:146	arg1	channels					155:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	3	15	theme	HeptaKO	453:459	arg1	mice					461:464	TRPC HeptaKO mice	448:464	TRPC HeptaKO mice	448:464	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	3	15	theme	HeptaKO	453:459	arg1	mice					442:445	mice	442:445	mice (TRPC HeptaKO mice)	442:465	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	4	16	theme	TRPC	638:641	arg1	mice					651:654	DSS-treated TRPC HeptaKO mice	626:654	DSS-treated TRPC HeptaKO mice	626:654	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	4	17	theme	RNA-sequence	541:552	arg1	analysis					554:561	RNA-sequence analysis	541:561	RNA-sequence analysis	541:561	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	7	18	theme	DSS-treated	1118:1128	arg1	mice					1143:1146	DSS-treated TRPC HeptaKO mice	1118:1146	DSS-treated TRPC HeptaKO mice	1118:1146	In addition, the composition of gut microbiota was markedly disturbed in DSS-treated TRPC HeptaKO mice.
36592529	4	19	theme	DSS-treated	626:636	arg1	mice					651:654	DSS-treated TRPC HeptaKO mice	626:654	DSS-treated TRPC HeptaKO mice	626:654	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	5	20	theme	monocytes	791:799	arg1	infiltration					768:779	the infiltration	764:779	the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria	764:841	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	4	21	theme	chemokines	716:725	arg1	amount					665:670	an amount	662:670	an amount of increased pro-inflammatory cytokines and chemokines	662:725	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	4	21	theme	chemokines	716:725	arg1	chemokines					716:725	chemokines	716:725	chemokines	716:725	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	4	21	theme	chemokines	716:725	arg1	cytokines					702:710	increased pro-inflammatory cytokines	675:710	increased pro-inflammatory cytokines	675:710	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	4	22	theme	mice	651:654	arg1	colons					616:621	colons	616:621	colons of DSS-treated TRPC HeptaKO mice	616:654	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	0	23	theme	TRPC	0:3	arg1	absence					5:11	TRPC absence	0:11	TRPC absence	0:11	TRPC absence induces pro-inflammatory macrophages and gut microbe disorder, sensitizing mice to colitis.
36592529	8	24	theme	Abx	1184:1186	arg1	-treatment					1188:1197	antibiotic cocktail (Abx)-treatment	1163:1197	antibiotic cocktail (Abx)-treatment	1163:1197	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	4	25	theme	pro-inflammatory	685:700	arg1	cytokines					702:710	increased pro-inflammatory cytokines	675:710	increased pro-inflammatory cytokines	675:710	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	9	26	theme	DSS-induced	1374:1384	arg1	colitis					1386:1392	DSS-induced colitis	1374:1392	DSS-induced colitis	1374:1392	Collectively, these data suggest that ablation of all TRPCs promotes the development of DSS-induced colitis by inducing pro-inflammatory macrophages and gut microbiota disorder.
36592529	3	27	theme	-induced	523:530	arg1	colitis					532:538	dextran sulfate sodium (DSS)-induced colitis	495:538	dextran sulfate sodium (DSS)-induced colitis	495:538	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	4	28	theme	HeptaKO	643:649	arg1	mice					651:654	DSS-treated TRPC HeptaKO mice	626:654	DSS-treated TRPC HeptaKO mice	626:654	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	0	29	theme	pro-inflammatory	21:36	arg1	macrophages					38:48	pro-inflammatory macrophages	21:48	pro-inflammatory macrophages	21:48	TRPC absence induces pro-inflammatory macrophages and gut microbe disorder, sensitizing mice to colitis.
36592529	6	30	theme	M1	977:978	arg1	polarization					980:991	M1 polarization	977:991	M1 polarization	977:991	Results also revealed that macrophages from TRPC HeptaKO mice exhibited M1 polarization and enhanced secretion of pro-inflammatory factors.
36592529	7	31	theme	microbiota	1081:1090	arg1	composition					1062:1072	the composition	1058:1072	the composition of gut microbiota	1058:1090	In addition, the composition of gut microbiota was markedly disturbed in DSS-treated TRPC HeptaKO mice.
36592529	8	32	theme	WT	1257:1258	arg1	mice					1260:1263	WT mice	1257:1263	WT mice	1257:1263	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	1	33	theme	non-allelic	182:192	arg1	genes					194:198	seven non-allelic genes	176:198	seven non-allelic genes	176:198	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	8	34	theme	TRPC	1200:1203	arg1	mice					1213:1216	TRPC HeptaKO mice	1200:1216	TRPC HeptaKO mice	1200:1216	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	1	35	theme	various	276:282	arg1	diseases					284:291	various diseases	276:291	various diseases	276:291	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	6	36	from	mice	962:965	arg1	macrophages					932:942	macrophages	932:942	macrophages from TRPC HeptaKO mice	932:965	Results also revealed that macrophages from TRPC HeptaKO mice exhibited M1 polarization and enhanced secretion of pro-inflammatory factors.
36592529	5	37	theme	lamina	828:833	arg1	propria					835:841	colonic lamina propria	820:841	colonic lamina propria	820:841	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	4	38	from	profile	605:611	arg1	colons					616:621	colons	616:621	colons of DSS-treated TRPC HeptaKO mice	616:654	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	3	39	theme	TRPC	425:428	arg1	proteins					430:437	all seven TRPC proteins	415:437	all seven TRPC proteins	415:437	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	9	40	theme	pro-inflammatory	1406:1421	arg1	macrophages					1423:1433	pro-inflammatory macrophages	1406:1433	pro-inflammatory macrophages	1406:1433	Collectively, these data suggest that ablation of all TRPCs promotes the development of DSS-induced colitis by inducing pro-inflammatory macrophages and gut microbiota disorder.
36592529	6	41	theme	HeptaKO	954:960	arg1	mice					962:965	TRPC HeptaKO mice	949:965	TRPC HeptaKO mice	949:965	Results also revealed that macrophages from TRPC HeptaKO mice exhibited M1 polarization and enhanced secretion of pro-inflammatory factors.
36592529	7	42	theme	gut	1077:1079	arg1	microbiota					1081:1090	gut microbiota	1077:1090	gut microbiota	1077:1090	In addition, the composition of gut microbiota was markedly disturbed in DSS-treated TRPC HeptaKO mice.
36592529	5	43	theme	neutrophils	805:815	arg1	infiltration					768:779	the infiltration	764:779	the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria	764:841	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	3	44	theme	proteins	430:437	arg1	absence					404:410	absence	404:410	absence of all seven TRPC proteins in mice (TRPC HeptaKO mice)	404:465	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	0	45	theme	microbe	58:64	arg1	disorder					66:73	gut microbe disorder	54:73	gut microbe disorder	54:73	TRPC absence induces pro-inflammatory macrophages and gut microbe disorder, sensitizing mice to colitis.
36592529	6	46	theme	TRPC	949:952	arg1	mice					962:965	TRPC HeptaKO mice	949:965	TRPC HeptaKO mice	949:965	Results also revealed that macrophages from TRPC HeptaKO mice exhibited M1 polarization and enhanced secretion of pro-inflammatory factors.
36592529	5	47	theme	Ly6Chi	784:789	arg1	monocytes					791:799	Ly6Chi monocytes	784:799	Ly6Chi monocytes	784:799	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	1	48	dep	contributors	215:226	arg1	associated					260:269	associated	260:269	are strongly associated with various diseases	247:291	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	3	49	theme	colitis	532:538	arg1	development					480:490	the development	476:490	the development of dextran sulfate sodium (DSS)-induced colitis	476:538	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	2	50	theme	ablating	331:338	arg1	TRPCs					350:354	all seven TRPCs	340:354	ablating all seven TRPCs	331:354	Here we explored the consequences of ablating all seven TRPCs in mice focusing on colitis.
36592529	0	51	theme	gut	54:56	arg1	disorder					66:73	gut microbe disorder	54:73	gut microbe disorder	54:73	TRPC absence induces pro-inflammatory macrophages and gut microbe disorder, sensitizing mice to colitis.
36592529	1	52	theme	transient	109:117	arg1	contributors					215:226	important contributors	205:226	important contributors to calcium fluxes	205:244	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	1	52	theme	transient	109:117	arg1	channels					155:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	1	53	theme	TRPC	149:152	arg1	contributors					215:226	important contributors	205:226	important contributors to calcium fluxes	205:244	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	1	53	theme	TRPC	149:152	arg1	channels					155:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	8	54	with	differences	1240:1250	arg1	mice					1260:1263	WT mice	1257:1263	WT mice	1257:1263	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	1	55	theme	receptor	119:126	arg1	contributors					215:226	important contributors	205:226	important contributors to calcium fluxes	205:244	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	1	55	theme	receptor	119:126	arg1	channels					155:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	9	56	theme	colitis	1386:1392	arg1	development					1359:1369	the development	1355:1369	the development of DSS-induced colitis	1355:1392	Collectively, these data suggest that ablation of all TRPCs promotes the development of DSS-induced colitis by inducing pro-inflammatory macrophages and gut microbiota disorder.
36592529	4	57	theme	increased	675:683	arg1	cytokines					702:710	increased pro-inflammatory cytokines	675:710	increased pro-inflammatory cytokines	675:710	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	8	58	from	differences	1240:1250	arg1	severity					1276:1283	disease severity	1268:1283	disease severity	1268:1283	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	1	59	theme	potential	128:136	arg1	contributors					215:226	important contributors	205:226	important contributors to calcium fluxes	205:244	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	1	59	theme	potential	128:136	arg1	channels					155:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	5	60	theme	DSS-treated	874:884	arg1	mice					899:902	DSS-treated TRPC HeptaKO mice	874:902	DSS-treated TRPC HeptaKO mice	874:902	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	1	61	theme	important	205:213	arg1	contributors					215:226	important contributors	205:226	important contributors to calcium fluxes	205:244	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	1	61	theme	important	205:213	arg1	channels					155:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels	105:162	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
36592529	3	62	from	absence	404:410	arg1	mice					461:464	TRPC HeptaKO mice	448:464	TRPC HeptaKO mice	448:464	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	3	62	from	absence	404:410	arg1	mice					442:445	mice	442:445	mice (TRPC HeptaKO mice)	442:465	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	3	63	theme	TRPC	448:451	arg1	mice					461:464	TRPC HeptaKO mice	448:464	TRPC HeptaKO mice	448:464	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	3	63	theme	TRPC	448:451	arg1	mice					442:445	mice	442:445	mice (TRPC HeptaKO mice)	442:465	We discovered that absence of all seven TRPC proteins in mice (TRPC HeptaKO mice) promotes the development of dextran sulfate sodium (DSS)-induced colitis.
36592529	4	64	theme	cytokines	702:710	arg1	amount					665:670	an amount	662:670	an amount of increased pro-inflammatory cytokines and chemokines	662:725	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	4	64	theme	cytokines	702:710	arg1	chemokines					716:725	chemokines	716:725	chemokines	716:725	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	4	64	theme	cytokines	702:710	arg1	cytokines					702:710	increased pro-inflammatory cytokines	675:710	increased pro-inflammatory cytokines	675:710	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	5	65	theme	cytometry	733:741	arg1	analysis					743:750	Flow cytometry analysis	728:750	Flow cytometry analysis	728:750	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	5	66	theme	colonic	820:826	arg1	propria					835:841	colonic lamina propria	820:841	colonic lamina propria	820:841	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	5	67	theme	TRPC	886:889	arg1	mice					899:902	DSS-treated TRPC HeptaKO mice	874:902	DSS-treated TRPC HeptaKO mice	874:902	Flow cytometry analysis showed that the infiltration of Ly6Chi monocytes and neutrophils in colonic lamina propria was significantly increased in DSS-treated TRPC HeptaKO mice.
36592529	4	68	theme	pro-inflammatory	588:603	arg1	profile					605:611	an extremely pro-inflammatory profile	575:611	an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice	575:654	RNA-sequence analysis highlighted an extremely pro-inflammatory profile in colons of DSS-treated TRPC HeptaKO mice, with an amount of increased pro-inflammatory cytokines and chemokines.
36592529	8	69	theme	significant	1228:1238	arg1	differences					1240:1250	no significant differences	1225:1250	no significant differences with WT mice in disease severity	1225:1283	However, upon antibiotic cocktail (Abx)-treatment, TRPC HeptaKO mice showed no significant differences with WT mice in disease severity.
36592529	6	70	theme	pro-inflammatory	1019:1034	arg1	factors					1036:1042	pro-inflammatory factors	1019:1042	pro-inflammatory factors	1019:1042	Results also revealed that macrophages from TRPC HeptaKO mice exhibited M1 polarization and enhanced secretion of pro-inflammatory factors.
36592529	2	71	theme	TRPCs	350:354	arg1	consequences					315:326	the consequences	311:326	the consequences of ablating all seven TRPCs in mice	311:362	Here we explored the consequences of ablating all seven TRPCs in mice focusing on colitis.
36592529	1	72	theme	calcium	231:237	arg1	fluxes					239:244	calcium fluxes	231:244	calcium fluxes	231:244	The transient receptor potential canonical (TRPC) channels, encoded in seven non-allelic genes, are important contributors to calcium fluxes, are strongly associated with various diseases.
35032407	6	0	theme	micro-dissected	1108:1122	arg1	tissue					1124:1129	laser micro-dissected tissue	1102:1129	laser micro-dissected tissue	1102:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	8	1	theme	structural	1658:1667	arg1	properties					1684:1693	an altered structural and mechanical properties	1647:1693	an altered structural and mechanical properties of the CCZ in OA	1647:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	1	2	theme	subchondral	246:256	arg1	bone					258:261	the subchondral bone	242:261	the subchondral bone	242:261	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	8	3	theme	mechanical	1673:1682	arg1	properties					1684:1693	an altered structural and mechanical properties	1647:1693	an altered structural and mechanical properties of the CCZ in OA	1647:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	7	4	theme	binding	1275:1281	arg1	protein					1283:1289	cadherin binding protein	1266:1289	cadherin binding protein	1266:1289	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	4	5	theme	knee	835:838	arg1	surgery					852:858	knee replacement surgery	835:858	knee replacement surgery	835:858	We collected osteochondral tissue samples stratified according to disease severity, from 16 knee OA patients who underwent knee replacement surgery.
35032407	0	6	theme	biochemical	120:130	arg1	analysis					132:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	1	7	theme	unmineralized	380:392	arg1	cartilage					394:402	unmineralized cartilage	380:402	unmineralized cartilage	380:402	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	3	8	theme	molecular	583:591	arg1	composition					610:620	the structural, molecular, and biochemical composition	567:620	the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity	567:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	1	9	theme	underlying	411:420	arg1	bone					446:449	the underlying mineralized subchondral bone	407:449	the underlying mineralized subchondral bone	407:449	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	3	10	theme	structural	571:580	arg1	composition					610:620	the structural, molecular, and biochemical composition	567:620	the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity	567:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	8	11	theme	endochondral	1502:1513	arg1	ossification					1515:1526	the endochondral ossification	1498:1526	the endochondral ossification	1498:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	12	from	OA	1709:1710	arg1	properties					1684:1693	an altered structural and mechanical properties	1647:1693	an altered structural and mechanical properties of the CCZ in OA	1647:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	1	13	theme	subchondral	434:444	arg1	bone					446:449	the underlying mineralized subchondral bone	407:449	the underlying mineralized subchondral bone	407:449	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	9	14	theme	cartilage	1767:1775	arg1	changes					1777:1783	the calcified cartilage changes	1753:1783	the calcified cartilage changes	1753:1783	In conclusion, our results suggest that the calcified cartilage changes can reflect the disease progression.
35032407	7	15	theme	cadherin	1266:1273	arg1	protein					1283:1289	cadherin binding protein	1266:1289	cadherin binding protein	1266:1289	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	5	16	theme	rat	895:897	arg1	samples					899:905	meniscectomy-induced rat samples	874:905	meniscectomy-induced rat samples	874:905	We also used meniscectomy-induced rat samples to confirm the pathophysiologic changes of human samples.
35032407	6	17	theme	layer	1023:1027	arg1	characteristics					980:994	the characteristics	976:994	the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue	976:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	0	18	theme	osteoarthritis-A	76:91	arg1	analysis					132:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	6	19	theme	calcified	1003:1011	arg1	layer					1023:1027	the calcified cartilage layer	999:1027	the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue	999:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	2	20	from	hallmark	456:463	arg1	OA					507:508	OA	507:508	OA	507:508	The hallmark of CCZ characteristics in osteoarthritis (OA) is less well known.
35032407	2	20	from	hallmark	456:463	arg1	osteoarthritis					491:504	osteoarthritis	491:504	osteoarthritis (OA)	491:509	The hallmark of CCZ characteristics in osteoarthritis (OA) is less well known.
35032407	6	21	from	combination	1037:1047	arg1	tissue					1124:1129	laser micro-dissected tissue	1102:1129	laser micro-dissected tissue	1102:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	6	22	dep	morphological	1052:1064	arg1	proteomic					1080:1088	proteomic	1080:1088	proteomic	1080:1088	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	6	22	dep	morphological	1052:1064	arg1	biochemical					1067:1077	biochemical	1067:1077	biochemical	1067:1077	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	8	23	theme	elastic	1557:1563	arg1	module					1565:1570	the elastic module	1553:1570	the elastic module	1553:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	1	24	from	cartilage	394:402	arg1	support					367:373	biological and mechanical support	341:373	biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone	341:449	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	6	25	theme	analyses	1090:1097	arg1	combination					1037:1047	a combination	1035:1047	a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue	1035:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	3	26	from	composition	610:620	arg1	tissues					632:638	tissues	632:638	tissues affected by primary knee OA and its relationship with disease severity	632:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	7	27	theme	OA	1214:1215	arg1	progression					1217:1227	the OA progression	1210:1227	the OA progression	1210:1227	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	5	28	theme	human	950:954	arg1	samples					956:962	human samples	950:962	human samples	950:962	We also used meniscectomy-induced rat samples to confirm the pathophysiologic changes of human samples.
35032407	4	29	theme	tissue	739:744	arg1	samples					746:752	osteochondral tissue samples	725:752	osteochondral tissue samples stratified according to disease severity	725:793	We collected osteochondral tissue samples stratified according to disease severity, from 16 knee OA patients who underwent knee replacement surgery.
35032407	1	30	theme	important	276:284	arg1	role					286:289	an important role	273:289	an important role	273:289	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	7	31	theme	significant	1353:1363	arg1	changes					1365:1371	significant changes	1353:1371	significant changes	1353:1371	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	8	32	theme	collagen	1585:1592	arg1	direction					1594:1602	the collagen direction	1581:1602	the collagen direction	1581:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	4	33	theme	OA	809:810	arg1	patients					812:819	16 knee OA patients	801:819	16 knee OA patients who underwent knee replacement surgery	801:858	We collected osteochondral tissue samples stratified according to disease severity, from 16 knee OA patients who underwent knee replacement surgery.
35032407	0	34	theme	cartilage	31:39	arg1	integrity					41:49	calcified cartilage integrity	21:49	calcified cartilage integrity	21:49	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	2	35	theme	characteristics	472:486	arg1	hallmark					456:463	The hallmark	452:463	The hallmark of CCZ characteristics in osteoarthritis (OA)	452:509	The hallmark of CCZ characteristics in osteoarthritis (OA) is less well known.
35032407	3	36	theme	CCZ	625:627	arg1	composition					610:620	the structural, molecular, and biochemical composition	567:620	the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity	567:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	8	37	theme	collagen	1445:1452	arg1	occurrence					1484:1493	the occurrence	1480:1493	the occurrence of the endochondral ossification	1480:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	37	theme	collagen	1445:1452	arg1	thinning					1416:1423	thinning	1416:1423	thinning of the CCZ	1416:1434	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	37	theme	collagen	1445:1452	arg1	loss					1573:1576	loss	1573:1576	loss of the collagen direction	1573:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	37	theme	collagen	1445:1452	arg1	increase					1609:1616	increase	1609:1616	increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA	1609:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	37	theme	collagen	1445:1452	arg1	loss					1545:1548	loss	1545:1548	loss of the elastic module	1545:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	37	theme	collagen	1445:1452	arg1	loss					1437:1440	loss	1437:1440	loss of collagen and proteoglycan content	1437:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	37	theme	collagen	1445:1452	arg1	neovasculature					1529:1542	neovasculature	1529:1542	neovasculature	1529:1542	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	7	38	theme	Calcium/Phosphate	1166:1182	arg1	ratio					1184:1188	the Calcium/Phosphate ratio	1162:1188	the Calcium/Phosphate ratio	1162:1188	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	7	38	theme	Calcium/Phosphate	1166:1182	arg1	unchanged					1193:1201	unchanged	1193:1201	unchanged	1193:1201	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	7	39	theme	metabolism-related	1316:1333	arg1	proteins					1335:1342	carbohydrate metabolism-related proteins	1303:1342	carbohydrate metabolism-related proteins	1303:1342	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	3	40	theme	knee	660:663	arg1	OA					665:666	primary knee OA	652:666	primary knee OA	652:666	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	8	41	theme	proteoglycan	1458:1469	arg1	occurrence					1484:1493	the occurrence	1480:1493	the occurrence of the endochondral ossification	1480:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	41	theme	proteoglycan	1458:1469	arg1	thinning					1416:1423	thinning	1416:1423	thinning of the CCZ	1416:1434	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	41	theme	proteoglycan	1458:1469	arg1	loss					1573:1576	loss	1573:1576	loss of the collagen direction	1573:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	41	theme	proteoglycan	1458:1469	arg1	increase					1609:1616	increase	1609:1616	increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA	1609:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	41	theme	proteoglycan	1458:1469	arg1	loss					1545:1548	loss	1545:1548	loss of the elastic module	1545:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	41	theme	proteoglycan	1458:1469	arg1	loss					1437:1440	loss	1437:1440	loss of collagen and proteoglycan content	1437:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	41	theme	proteoglycan	1458:1469	arg1	neovasculature					1529:1542	neovasculature	1529:1542	neovasculature	1529:1542	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	1	42	theme	hyaline	210:216	arg1	cartilage					228:236	the hyaline articular cartilage	206:236	the hyaline articular cartilage	206:236	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	1	43	theme	cartilage	156:164	arg1	interlayer					187:196	a thin interlayer	180:196	a thin interlayer between the hyaline articular cartilage and the subchondral bone	180:261	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	1	43	theme	cartilage	156:164	arg1	CCZ					172:174	CCZ	172:174	CCZ	172:174	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	1	43	theme	cartilage	156:164	arg1	zone					166:169	The calcified cartilage zone	142:169	The calcified cartilage zone (CCZ)	142:175	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	8	44	theme	CCZ	1432:1434	arg1	occurrence					1484:1493	the occurrence	1480:1493	the occurrence of the endochondral ossification	1480:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	44	theme	CCZ	1432:1434	arg1	thinning					1416:1423	thinning	1416:1423	thinning of the CCZ	1416:1434	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	44	theme	CCZ	1432:1434	arg1	loss					1573:1576	loss	1573:1576	loss of the collagen direction	1573:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	44	theme	CCZ	1432:1434	arg1	increase					1609:1616	increase	1609:1616	increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA	1609:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	44	theme	CCZ	1432:1434	arg1	loss					1545:1548	loss	1545:1548	loss of the elastic module	1545:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	44	theme	CCZ	1432:1434	arg1	loss					1437:1440	loss	1437:1440	loss of collagen and proteoglycan content	1437:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	44	theme	CCZ	1432:1434	arg1	neovasculature					1529:1542	neovasculature	1529:1542	neovasculature	1529:1542	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	7	45	theme	calcium-binding	1238:1252	arg1	protein					1254:1260	the calcium-binding protein	1234:1260	the calcium-binding protein	1234:1260	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	6	46	from	tissue	1124:1129	arg1	combination					1037:1047	a combination	1035:1047	a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue	1035:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	6	47	theme	laser	1102:1106	arg1	tissue					1124:1129	laser micro-dissected tissue	1102:1129	laser micro-dissected tissue	1102:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	8	48	theme	altered	1650:1656	arg1	properties					1684:1693	an altered structural and mechanical properties	1647:1693	an altered structural and mechanical properties of the CCZ in OA	1647:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	3	49	with	relationship	676:687	arg1	severity					702:709	disease severity	694:709	disease severity	694:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	6	50	from	analyses	1090:1097	arg1	tissue					1124:1129	laser micro-dissected tissue	1102:1129	laser micro-dissected tissue	1102:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	3	51	theme	disease	694:700	arg1	severity					702:709	disease severity	694:709	disease severity	694:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	5	52	used	used	869:872	arg2	We					861:862	We	861:862	We	861:862	We also used meniscectomy-induced rat samples to confirm the pathophysiologic changes of human samples.
35032407	4	53	theme	replacement	840:850	arg1	surgery					852:858	knee replacement surgery	835:858	knee replacement surgery	835:858	We collected osteochondral tissue samples stratified according to disease severity, from 16 knee OA patients who underwent knee replacement surgery.
35032407	0	54	theme	analysis	132:139	arg1	severity					64:71	the severity	60:71	the severity of osteoarthritis-A structural, molecular, and biochemical analysis	60:139	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	3	55	theme	study	546:550	arg1	aim					535:537	The aim	531:537	The aim of our study	531:550	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	1	56	theme	thin	182:185	arg1	interlayer					187:196	a thin interlayer	180:196	a thin interlayer between the hyaline articular cartilage and the subchondral bone	180:261	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	1	56	theme	thin	182:185	arg1	zone					166:169	The calcified cartilage zone	142:169	The calcified cartilage zone (CCZ)	142:175	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	1	57	theme	calcified	146:154	arg1	interlayer					187:196	a thin interlayer	180:196	a thin interlayer between the hyaline articular cartilage and the subchondral bone	180:261	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	1	57	theme	calcified	146:154	arg1	CCZ					172:174	CCZ	172:174	CCZ	172:174	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	1	57	theme	calcified	146:154	arg1	zone					166:169	The calcified cartilage zone	142:169	The calcified cartilage zone (CCZ)	142:175	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	8	58	theme	ossification	1515:1526	arg1	occurrence					1484:1493	the occurrence	1480:1493	the occurrence of the endochondral ossification	1480:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	58	theme	ossification	1515:1526	arg1	thinning					1416:1423	thinning	1416:1423	thinning of the CCZ	1416:1434	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	58	theme	ossification	1515:1526	arg1	loss					1573:1576	loss	1573:1576	loss of the collagen direction	1573:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	58	theme	ossification	1515:1526	arg1	increase					1609:1616	increase	1609:1616	increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA	1609:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	58	theme	ossification	1515:1526	arg1	loss					1545:1548	loss	1545:1548	loss of the elastic module	1545:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	58	theme	ossification	1515:1526	arg1	loss					1437:1440	loss	1437:1440	loss of collagen and proteoglycan content	1437:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	58	theme	ossification	1515:1526	arg1	neovasculature					1529:1542	neovasculature	1529:1542	neovasculature	1529:1542	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	1	59	theme	mineralized	422:432	arg1	bone					446:449	the underlying mineralized subchondral bone	407:449	the underlying mineralized subchondral bone	407:449	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	3	60	theme	biochemical	598:608	arg1	composition					610:620	the structural, molecular, and biochemical composition	567:620	the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity	567:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	5	61	theme	pathophysiologic	922:937	arg1	changes					939:945	the pathophysiologic changes	918:945	the pathophysiologic changes of human samples	918:962	We also used meniscectomy-induced rat samples to confirm the pathophysiologic changes of human samples.
35032407	9	62	theme	calcified	1757:1765	arg1	changes					1777:1783	the calcified cartilage changes	1753:1783	the calcified cartilage changes	1753:1783	In conclusion, our results suggest that the calcified cartilage changes can reflect the disease progression.
35032407	8	63	from	CCZ	1702:1704	arg1	OA					1709:1710	OA	1709:1710	OA	1709:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	5	64	theme	meniscectomy-induced	874:893	arg1	samples					899:905	meniscectomy-induced rat samples	874:905	meniscectomy-induced rat samples	874:905	We also used meniscectomy-induced rat samples to confirm the pathophysiologic changes of human samples.
35032407	0	65	theme	structural	93:102	arg1	analysis					132:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	8	66	dep	collagen	1445:1452	arg1	content					1471:1477	content	1471:1477	content	1471:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	6	67	theme	cartilage	1013:1021	arg1	layer					1023:1027	the calcified cartilage layer	999:1027	the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue	999:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	0	68	theme	molecular	105:113	arg1	analysis					132:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	osteoarthritis-A structural, molecular, and biochemical analysis	76:139	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	1	69	theme	biological	341:350	arg1	support					367:373	biological and mechanical support	341:373	biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone	341:449	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	9	70	theme	disease	1801:1807	arg1	progression					1809:1819	the disease progression	1797:1819	the disease progression	1797:1819	In conclusion, our results suggest that the calcified cartilage changes can reflect the disease progression.
35032407	8	71	theme	module	1565:1570	arg1	occurrence					1484:1493	the occurrence	1480:1493	the occurrence of the endochondral ossification	1480:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	71	theme	module	1565:1570	arg1	thinning					1416:1423	thinning	1416:1423	thinning of the CCZ	1416:1434	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	71	theme	module	1565:1570	arg1	loss					1573:1576	loss	1573:1576	loss of the collagen direction	1573:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	71	theme	module	1565:1570	arg1	increase					1609:1616	increase	1609:1616	increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA	1609:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	71	theme	module	1565:1570	arg1	loss					1545:1548	loss	1545:1548	loss of the elastic module	1545:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	71	theme	module	1565:1570	arg1	loss					1437:1440	loss	1437:1440	loss of collagen and proteoglycan content	1437:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	71	theme	module	1565:1570	arg1	neovasculature					1529:1542	neovasculature	1529:1542	neovasculature	1529:1542	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	1	72	theme	mechanical	356:365	arg1	support					367:373	biological and mechanical support	341:373	biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone	341:449	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	8	73	from	properties	1684:1693	arg1	OA					1709:1710	OA	1709:1710	OA	1709:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	0	74	theme	calcified	21:29	arg1	integrity					41:49	calcified cartilage integrity	21:49	calcified cartilage integrity	21:49	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	5	75	theme	samples	956:962	arg1	changes					939:945	the pathophysiologic changes	918:945	the pathophysiologic changes of human samples	918:962	We also used meniscectomy-induced rat samples to confirm the pathophysiologic changes of human samples.
35032407	8	76	theme	direction	1594:1602	arg1	occurrence					1484:1493	the occurrence	1480:1493	the occurrence of the endochondral ossification	1480:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	76	theme	direction	1594:1602	arg1	thinning					1416:1423	thinning	1416:1423	thinning of the CCZ	1416:1434	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	76	theme	direction	1594:1602	arg1	loss					1573:1576	loss	1573:1576	loss of the collagen direction	1573:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	76	theme	direction	1594:1602	arg1	increase					1609:1616	increase	1609:1616	increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA	1609:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	76	theme	direction	1594:1602	arg1	loss					1545:1548	loss	1545:1548	loss of the elastic module	1545:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	76	theme	direction	1594:1602	arg1	loss					1437:1440	loss	1437:1440	loss of collagen and proteoglycan content	1437:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	76	theme	direction	1594:1602	arg1	neovasculature					1529:1542	neovasculature	1529:1542	neovasculature	1529:1542	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	4	77	theme	osteochondral	725:737	arg1	samples					746:752	osteochondral tissue samples	725:752	osteochondral tissue samples stratified according to disease severity	725:793	We collected osteochondral tissue samples stratified according to disease severity, from 16 knee OA patients who underwent knee replacement surgery.
35032407	0	78	theme	integrity	41:49	arg1	deterioration					4:16	The deterioration	0:16	The deterioration of calcified cartilage integrity	0:49	The deterioration of calcified cartilage integrity reflects the severity of osteoarthritis-A structural, molecular, and biochemical analysis.
35032407	6	79	theme	morphological	1052:1064	arg1	analyses					1090:1097	morphological, biochemical, proteomic analyses	1052:1097	morphological, biochemical, proteomic analyses on laser micro-dissected tissue	1052:1129	We defined the characteristics of the calcified cartilage layer using a combination of morphological, biochemical, proteomic analyses on laser micro-dissected tissue.
35032407	4	80	theme	16 knee	801:807	arg1	patients					812:819	16 knee OA patients	801:819	16 knee OA patients who underwent knee replacement surgery	801:858	We collected osteochondral tissue samples stratified according to disease severity, from 16 knee OA patients who underwent knee replacement surgery.
35032407	7	81	theme	carbohydrate	1303:1314	arg1	proteins					1335:1342	carbohydrate metabolism-related proteins	1303:1342	carbohydrate metabolism-related proteins	1303:1342	Our results demonstrated that the Calcium/Phosphate ratio is unchanged during the OA progression, but the calcium-binding protein and cadherin binding protein, as well as carbohydrate metabolism-related proteins, undergo significant changes.
35032407	2	82	theme	CCZ	468:470	arg1	characteristics					472:486	CCZ characteristics	468:486	CCZ characteristics	468:486	The hallmark of CCZ characteristics in osteoarthritis (OA) is less well known.
35032407	3	83	theme	primary	652:658	arg1	OA					665:666	primary knee OA	652:666	primary knee OA	652:666	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
35032407	1	84	theme	joint	310:314	arg1	homeostasis					316:326	the joint homeostasis	306:326	the joint homeostasis	306:326	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	8	85	theme	tortuosity	1625:1634	arg1	occurrence					1484:1493	the occurrence	1480:1493	the occurrence of the endochondral ossification	1480:1526	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	85	theme	tortuosity	1625:1634	arg1	thinning					1416:1423	thinning	1416:1423	thinning of the CCZ	1416:1434	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	85	theme	tortuosity	1625:1634	arg1	loss					1573:1576	loss	1573:1576	loss of the collagen direction	1573:1602	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	85	theme	tortuosity	1625:1634	arg1	increase					1609:1616	increase	1609:1616	increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA	1609:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	85	theme	tortuosity	1625:1634	arg1	loss					1545:1548	loss	1545:1548	loss of the elastic module	1545:1570	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	85	theme	tortuosity	1625:1634	arg1	loss					1437:1440	loss	1437:1440	loss of collagen and proteoglycan content	1437:1477	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	8	85	theme	tortuosity	1625:1634	arg1	neovasculature					1529:1542	neovasculature	1529:1542	neovasculature	1529:1542	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	4	86	theme	disease	778:784	arg1	severity					786:793	disease severity	778:793	disease severity	778:793	We collected osteochondral tissue samples stratified according to disease severity, from 16 knee OA patients who underwent knee replacement surgery.
35032407	8	87	theme	CCZ	1702:1704	arg1	properties					1684:1693	an altered structural and mechanical properties	1647:1693	an altered structural and mechanical properties of the CCZ in OA	1647:1710	These changes were further accompanied by thinning of the CCZ, loss of collagen and proteoglycan content, the occurrence of the endochondral ossification, neovasculature, loss of the elastic module, loss of the collagen direction, and increase of the tortuosity indicating an altered structural and mechanical properties of the CCZ in OA.
35032407	1	88	theme	articular	218:226	arg1	cartilage					228:236	the hyaline articular cartilage	206:236	the hyaline articular cartilage	206:236	The calcified cartilage zone (CCZ) is a thin interlayer between the hyaline articular cartilage and the subchondral bone and plays an important role in maintaining the joint homeostasis by providing biological and mechanical support from unmineralized cartilage to the underlying mineralized subchondral bone.
35032407	3	89	with	OA	665:666	arg1	severity					702:709	disease severity	694:709	disease severity	694:709	The aim of our study is to evaluate the structural, molecular, and biochemical composition of CCZ in tissues affected by primary knee OA and its relationship with disease severity.
36167020	0	0	theme	chicken	119:125	arg1	line					143:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	7	1	theme	Wnt	1360:1362	arg1	pathways					1374:1381	Wnt signaling pathways	1360:1381	Wnt signaling pathways	1360:1381	KEGG pathway analysis showed that most of the target genes were related to mitogen-activated protein kinase and Wnt signaling pathways.
36167020	7	2	theme	mitogen-activated	1323:1339	arg1	kinase					1349:1354	mitogen-activated protein kinase	1323:1354	mitogen-activated protein kinase	1323:1354	KEGG pathway analysis showed that most of the target genes were related to mitogen-activated protein kinase and Wnt signaling pathways.
36167020	3	3	theme	exosomal	550:557	arg1	miRNAs					559:564	exosomal miRNAs	550:564	exosomal miRNAs	550:564	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	8	4	theme	sequencing	1548:1557	arg1	results					1559:1565	the sequencing results	1544:1565	the sequencing results	1544:1565	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	5	5	from	miRNAs	1147:1152	arg1	exosomes					1103:1110	poly(I:C)-stimulated exosomes	1082:1110	poly(I:C)-stimulated exosomes (POLY-EXO)	1082:1121	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	5	from	miRNAs	1147:1152	arg1	POLY-EXO					1113:1120	POLY-EXO	1113:1120	POLY-EXO	1113:1120	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	5	from	miRNAs	1147:1152	arg1	CTRL-EXO					1127:1134	CTRL-EXO	1127:1134	CTRL-EXO	1127:1134	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	0	6	theme	acid-stimulated	103:117	arg1	line					143:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	7	7	theme	protein	1341:1347	arg1	kinase					1349:1354	mitogen-activated protein kinase	1323:1354	mitogen-activated protein kinase	1323:1354	KEGG pathway analysis showed that most of the target genes were related to mitogen-activated protein kinase and Wnt signaling pathways.
36167020	8	8	dep	miRNAs	1417:1422	arg1	gga-miR-338-3p					1489:1502	gga-miR-338-3p	1489:1502	gga-miR-338-3p	1489:1502	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	8	8	dep	miRNAs	1417:1422	arg1	gga-miR-19a-3p					1441:1454	gga-miR-19a-3p	1441:1454	gga-miR-19a-3p	1441:1454	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	8	8	dep	miRNAs	1417:1422	arg1	gga-miR-21-3p					1457:1469	gga-miR-21-3p	1457:1469	gga-miR-21-3p	1457:1469	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	8	8	dep	miRNAs	1417:1422	arg1	gga-miR-3523					1509:1520	gga-miR-3523	1509:1520	gga-miR-3523	1509:1520	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	8	8	dep	miRNAs	1417:1422	arg1	miRNAs					1417:1422	miRNAs	1417:1422	miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523)	1417:1521	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	8	8	dep	miRNAs	1417:1422	arg1	gga-miR-142-3p					1425:1438	gga-miR-142-3p	1425:1438	gga-miR-142-3p	1425:1438	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	8	8	dep	miRNAs	1417:1422	arg1	gga-miR-301a-3p					1472:1486	gga-miR-301a-3p	1472:1486	gga-miR-301a-3p	1472:1486	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	5	9	from	exosomes	1009:1016	arg1	exosomes					1103:1110	poly(I:C)-stimulated exosomes	1082:1110	poly(I:C)-stimulated exosomes (POLY-EXO)	1082:1121	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	9	from	exosomes	1009:1016	arg1	POLY-EXO					1113:1120	POLY-EXO	1113:1120	POLY-EXO	1113:1120	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	9	from	exosomes	1009:1016	arg1	CTRL-EXO					1127:1134	CTRL-EXO	1127:1134	CTRL-EXO	1127:1134	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	3	10	theme	miRNAs	559:564	arg1	profiles					538:545	profiles	538:545	profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11)	538:689	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	8	11	with	consistent	1528:1537	arg1	results					1559:1565	the sequencing results	1544:1565	the sequencing results	1544:1565	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	5	12	theme	-stimulated	1091:1101	arg1	exosomes					1103:1110	poly(I:C)-stimulated exosomes	1082:1110	poly(I:C)-stimulated exosomes (POLY-EXO)	1082:1121	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	12	theme	-stimulated	1091:1101	arg1	POLY-EXO					1113:1120	POLY-EXO	1113:1120	POLY-EXO	1113:1120	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	0	13	theme	cell	138:141	arg1	line					143:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	3	14	theme	small	708:712	arg1	sequencing					718:727	small RNA sequencing	708:727	small RNA sequencing	708:727	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	5	15	theme	I	1087:1087	arg1	poly					1082:1085	poly	1082:1085	poly(I:C)-stimulated exosomes (POLY-EXO)	1082:1121	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	15	theme	I	1087:1087	arg1	C					1089:1089	I:C	1087:1089	I:C	1087:1089	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	7	16	theme	target	1294:1299	arg1	genes					1301:1305	the target genes	1290:1305	the target genes	1290:1305	KEGG pathway analysis showed that most of the target genes were related to mitogen-activated protein kinase and Wnt signaling pathways.
36167020	5	17	theme	DE	1144:1145	arg1	miRNAs					1147:1152	45 DE miRNAs	1141:1152	45 DE miRNAs	1141:1152	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	0	18	theme	macrophage	127:136	arg1	line					143:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	5	19	from	miRNAs	1072:1077	arg1	exosomes					1103:1110	poly(I:C)-stimulated exosomes	1082:1110	poly(I:C)-stimulated exosomes (POLY-EXO)	1082:1121	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	19	from	miRNAs	1072:1077	arg1	POLY-EXO					1113:1120	POLY-EXO	1113:1120	POLY-EXO	1113:1120	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	19	from	miRNAs	1072:1077	arg1	CTRL-EXO					1127:1134	CTRL-EXO	1127:1134	CTRL-EXO	1127:1134	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	2	20	theme	biological	326:335	arg1	processes					337:345	various biological processes	318:345	various biological processes in recipient cells	318:364	In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression.
36167020	3	21	from	line	679:682	arg1	profiles					538:545	profiles	538:545	profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11)	538:689	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	5	22	theme	unstimulated	1032:1043	arg1	exosomes					1045:1052	unstimulated exosomes	1032:1052	unstimulated exosomes	1032:1052	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	3	23	theme	viral	520:524	arg1	infections					526:535	bacterial and viral infections	506:535	bacterial and viral infections	506:535	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	2	24	theme	various	318:324	arg1	processes					337:345	various biological processes	318:345	various biological processes in recipient cells	318:364	In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression.
36167020	3	25	theme	regulatory	476:485	arg1	mechanisms					487:496	their regulatory mechanisms	470:496	their regulatory mechanisms against bacterial and viral infections	470:535	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	4	26	theme	HiSeq	893:897	arg1	System					904:909	the HiSeq 2500 System	889:909	the HiSeq 2500 System	889:909	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	8	27	theme	qRT-PCR	1405:1411	arg1	results					1394:1400	results	1394:1400	results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523)	1394:1521	Moreover, results of qRT-PCR for miRNAs (gga-miR-142-3p, gga-miR-19a-3p, gga-miR-21-3p, gga-miR-301a-3p, gga-miR-338-3p, and gga-miR-3523) were consistent with the sequencing results.
36167020	9	28	theme	viral	1732:1736	arg1	infections					1738:1747	bacterial and viral infections	1718:1747	bacterial and viral infections	1718:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	9	29	attach	derived	1656:1662	arg1	macrophages					1669:1679	macrophages	1669:1679	macrophages against pathological insults such as bacterial and viral infections	1669:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	9	29	attach	derived	1656:1662	arg2	miRNAs					1649:1654	exosomal miRNAs	1640:1654	exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections	1640:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	1	30	theme	exosomal	219:226	arg1	proteins					228:235	exosomal proteins	219:235	exosomal proteins	219:235	Exosomes play important roles in cellular communication by delivering exosomal proteins and nucleic acid molecules to cells.
36167020	5	31	theme	expressed	938:946	arg1	miRNAs					948:953	Thirty six differentially expressed miRNAs	912:953	Thirty six differentially expressed miRNAs (DE miRNAs)	912:965	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	31	theme	expressed	938:946	arg1	miRNAs					959:964	DE miRNAs	956:964	DE miRNAs	956:964	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	9	32	theme	exosomal	1640:1647	arg1	miRNAs					1649:1654	exosomal miRNAs	1640:1654	exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections	1640:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	9	33	theme	immuno-regulatory	1608:1624	arg1	mechanisms					1626:1635	immuno-regulatory mechanisms	1608:1635	immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections	1608:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	6	34	theme	Target	1179:1184	arg1	genes					1186:1190	Target genes	1179:1190	Target genes of DE miRNAs	1179:1203	Target genes of DE miRNAs were predicted using miRDB and TargetScan.
36167020	0	35	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of exosomal miRNAs	0:38	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	0	36	theme	exosomal	24:31	arg1	miRNAs					33:38	exosomal miRNAs	24:38	exosomal miRNAs	24:38	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	4	37	theme	I	799:799	arg1	C					801:801	I:C	799:801	I:C	799:801	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	37	theme	I	799:799	arg1	poly					794:797	poly	794:797	poly(I:C) (50 μg/mL)	794:813	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	38	theme	small	862:866	arg1	sequencing					872:881	small RNA sequencing	862:881	small RNA sequencing	862:881	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	9	39	theme	bacterial	1718:1726	arg1	infections					1738:1747	bacterial and viral infections	1718:1747	bacterial and viral infections	1718:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	2	40	theme	recipient	350:358	arg1	cells					360:364	recipient cells	350:364	recipient cells	350:364	In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression.
36167020	7	41	theme	KEGG	1248:1251	arg1	analysis					1261:1268	KEGG pathway analysis	1248:1268	KEGG pathway analysis	1248:1268	KEGG pathway analysis showed that most of the target genes were related to mitogen-activated protein kinase and Wnt signaling pathways.
36167020	1	42	theme	nucleic	241:247	arg1	molecules					254:262	nucleic acid molecules	241:262	nucleic acid molecules	241:262	Exosomes play important roles in cellular communication by delivering exosomal proteins and nucleic acid molecules to cells.
36167020	5	43	from	exosomes	1045:1052	arg1	exosomes					1103:1110	poly(I:C)-stimulated exosomes	1082:1110	poly(I:C)-stimulated exosomes (POLY-EXO)	1082:1121	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	43	from	exosomes	1045:1052	arg1	POLY-EXO					1113:1120	POLY-EXO	1113:1120	POLY-EXO	1113:1120	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	43	from	exosomes	1045:1052	arg1	CTRL-EXO					1127:1134	CTRL-EXO	1127:1134	CTRL-EXO	1127:1134	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	6	44	theme	miRNAs	1198:1203	arg1	genes					1186:1190	Target genes	1179:1190	Target genes of DE miRNAs	1179:1203	Target genes of DE miRNAs were predicted using miRDB and TargetScan.
36167020	1	45	theme	acid	249:252	arg1	molecules					254:262	nucleic acid molecules	241:262	nucleic acid molecules	241:262	Exosomes play important roles in cellular communication by delivering exosomal proteins and nucleic acid molecules to cells.
36167020	3	46	theme	exosomal	450:457	arg1	miRNAs					459:464	exosomal miRNAs	450:464	exosomal miRNAs	450:464	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	6	47	theme	DE	1195:1196	arg1	miRNAs					1198:1203	DE miRNAs	1195:1203	DE miRNAs	1195:1203	Target genes of DE miRNAs were predicted using miRDB and TargetScan.
36167020	5	48	theme	DE	956:957	arg1	miRNAs					948:953	Thirty six differentially expressed miRNAs	912:953	Thirty six differentially expressed miRNAs (DE miRNAs)	912:965	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	48	theme	DE	956:957	arg1	miRNAs					959:964	DE miRNAs	956:964	DE miRNAs	956:964	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	0	49	theme	miRNAs	33:38	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of exosomal miRNAs	0:38	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	5	50	theme	DE	1069:1070	arg1	miRNAs					1072:1077	42 DE miRNAs	1066:1077	42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO	1066:1134	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	3	51	theme	miRNAs	459:464	arg1	mechanisms					487:496	their regulatory mechanisms	470:496	their regulatory mechanisms against bacterial and viral infections	470:535	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	51	theme	miRNAs	459:464	arg1	composition					435:445	the composition	431:445	the composition of exosomal miRNAs	431:464	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	52	theme	macrophage	663:672	arg1	line					679:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line	571:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11)	571:689	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	52	theme	macrophage	663:672	arg1	HD11					685:688	HD11	685:688	HD11	685:688	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	7	53	theme	pathway	1253:1259	arg1	analysis					1261:1268	KEGG pathway analysis	1248:1268	KEGG pathway analysis	1248:1268	KEGG pathway analysis showed that most of the target genes were related to mitogen-activated protein kinase and Wnt signaling pathways.
36167020	9	54	theme	pathological	1689:1700	arg1	infections					1738:1747	bacterial and viral infections	1718:1747	bacterial and viral infections	1718:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	9	54	theme	pathological	1689:1700	arg1	insults					1702:1708	pathological insults	1689:1708	pathological insults such as bacterial and viral infections	1689:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	3	55	theme	bacterial	506:514	arg1	infections					526:535	bacterial and viral infections	506:535	bacterial and viral infections	506:535	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	4	56	dep	h.	822:823	arg1	analyzed					849:856	analyzed	849:856	were analyzed for small RNA sequencing using the HiSeq 2500 System	844:909	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	3	57	theme	cell	674:677	arg1	line					679:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line	571:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11)	571:689	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	57	theme	cell	674:677	arg1	HD11					685:688	HD11	685:688	HD11	685:688	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	4	58	theme	RNA	868:870	arg1	sequencing					872:881	small RNA sequencing	862:881	small RNA sequencing	862:881	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	59	with	stimulation	759:769	arg1	C					801:801	I:C	799:801	I:C	799:801	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	59	with	stimulation	759:769	arg1	LPS					776:778	LPS	776:778	LPS (1 μg/mL)	776:788	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	59	with	stimulation	759:769	arg1	μg/mL					783:787	1 μg/mL	781:787	1 μg/mL	781:787	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	59	with	stimulation	759:769	arg1	poly					794:797	poly	794:797	poly(I:C) (50 μg/mL)	794:813	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	59	with	stimulation	759:769	arg1	μg/mL					808:812	50 μg/mL	805:812	50 μg/mL	805:812	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	4	60	theme	exosomal	831:838	arg1	RNA					840:842	exosomal RNA	831:842	exosomal RNA	831:842	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	3	61	theme	I	638:638	arg1	C					640:640	I:C	638:640	I:C	638:640	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	61	theme	I	638:638	arg1	poly					633:636	poly	633:636	poly(I:C)	633:641	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	2	62	theme	gene	387:390	arg1	expression					392:401	target gene expression	380:401	target gene expression	380:401	In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression.
36167020	4	63	theme	2500	899:902	arg1	System					904:909	the HiSeq 2500 System	889:909	the HiSeq 2500 System	889:909	Exosomes were purified after stimulation with LPS (1 μg/mL) and poly(I:C) (50 μg/mL) for 24 h. Then, exosomal RNA were analyzed for small RNA sequencing using the HiSeq 2500 System.
36167020	5	64	theme	poly	1082:1085	arg1	exosomes					1103:1110	poly(I:C)-stimulated exosomes	1082:1110	poly(I:C)-stimulated exosomes (POLY-EXO)	1082:1121	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	64	theme	poly	1082:1085	arg1	POLY-EXO					1113:1120	POLY-EXO	1113:1120	POLY-EXO	1113:1120	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	3	65	theme	-stimulated	643:653	arg1	line					679:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line	571:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11)	571:689	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	65	theme	-stimulated	643:653	arg1	HD11					685:688	HD11	685:688	HD11	685:688	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	1	66	theme	important	163:171	arg1	roles					173:177	important roles	163:177	important roles	163:177	Exosomes play important roles in cellular communication by delivering exosomal proteins and nucleic acid molecules to cells.
36167020	3	67	theme	polyinosinic-polycytidylic	600:625	arg1	acid					627:630	polyinosinic-polycytidylic acid	600:630	polyinosinic-polycytidylic acid (poly(I:C))	600:642	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	7	68	theme	signaling	1364:1372	arg1	pathways					1374:1381	Wnt signaling pathways	1360:1381	Wnt signaling pathways	1360:1381	KEGG pathway analysis showed that most of the target genes were related to mitogen-activated protein kinase and Wnt signaling pathways.
36167020	3	69	theme	chicken	655:661	arg1	line					679:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line	571:682	lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11)	571:689	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	69	theme	chicken	655:661	arg1	HD11					685:688	HD11	685:688	HD11	685:688	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	70	dep	acid	627:630	arg1	C					640:640	I:C	638:640	I:C	638:640	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	3	70	dep	acid	627:630	arg1	poly					633:636	poly	633:636	poly(I:C)	633:641	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	2	71	from	processes	337:345	arg1	cells					360:364	recipient cells	350:364	recipient cells	350:364	In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression.
36167020	2	72	theme	target	380:385	arg1	expression					392:401	target gene expression	380:401	target gene expression	380:401	In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression.
36167020	0	73	theme	polyinosinic-polycytidylic	76:101	arg1	line					143:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line	76:146	Comparative analysis of exosomal miRNAs derived from lipopolysaccharide and polyinosinic-polycytidylic acid-stimulated chicken macrophage cell line.
36167020	5	74	theme	LPS-stimulated	994:1007	arg1	LPS-EXO					1019:1025	LPS-EXO	1019:1025	LPS-EXO	1019:1025	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	74	theme	LPS-stimulated	994:1007	arg1	CTRL-EXO					1055:1062	CTRL-EXO	1055:1062	CTRL-EXO	1055:1062	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	5	74	theme	LPS-stimulated	994:1007	arg1	exosomes					1009:1016	LPS-stimulated exosomes	994:1016	LPS-stimulated exosomes (LPS-EXO)	994:1026	Thirty six differentially expressed miRNAs (DE miRNAs) were obtained by comparing LPS-stimulated exosomes (LPS-EXO) and unstimulated exosomes (CTRL-EXO), 42 DE miRNAs in poly(I:C)-stimulated exosomes (POLY-EXO) and CTRL-EXO, and 45 DE miRNAs in LPS-EXO and POLY-EXO.
36167020	2	75	theme	exosomal	289:296	arg1	miRNAs					298:303	exosomal miRNAs	289:303	exosomal miRNAs	289:303	In particular, exosomal miRNAs can modulate various biological processes in recipient cells by repressing target gene expression.
36167020	9	76	theme	miRNAs	1649:1654	arg1	mechanisms					1626:1635	immuno-regulatory mechanisms	1608:1635	immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections	1608:1747	This study will provide knowledge about immuno-regulatory mechanisms of exosomal miRNAs derived from macrophages against pathological insults such as bacterial and viral infections.
36167020	3	77	theme	RNA	714:716	arg1	sequencing					718:727	small RNA sequencing	708:727	small RNA sequencing	708:727	In this study, to identify the composition of exosomal miRNAs and their regulatory mechanisms against bacterial and viral infections, profiles of exosomal miRNAs from lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly(I:C))-stimulated chicken macrophage cell line (HD11) were analyzed by small RNA sequencing.
36167020	1	78	theme	cellular	182:189	arg1	communication					191:203	cellular communication	182:203	cellular communication	182:203	Exosomes play important roles in cellular communication by delivering exosomal proteins and nucleic acid molecules to cells.
36037991	7	0	from	levels	1405:1410	arg1	mice					1442:1445	mice	1442:1445	mice	1442:1445	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	2	1	theme	anti-inflammatory	378:394	arg1	effects					396:402	anti-inflammatory effects	378:402	anti-inflammatory effects of the vagus nerve which plays a role in depression	378:454	The spleen mediates anti-inflammatory effects of the vagus nerve which plays a role in depression.
36037991	4	2	from	mice	711:714	arg1	composition					672:682	abnormal composition	663:682	abnormal composition	663:682	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	2	from	mice	711:714	arg1	phenotype					625:633	the depression-like phenotype	605:633	the depression-like phenotype	605:633	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	2	from	mice	711:714	arg1	inflammation					645:656	systemic inflammation	636:656	systemic inflammation	636:656	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	3	theme	depression-like	609:623	arg1	phenotype					625:633	the depression-like phenotype	605:633	the depression-like phenotype	605:633	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	1	4	theme	axis	280:283	arg1	role					259:262	the role	255:262	the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression	255:355	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	6	5	theme	SND-operated	1194:1205	arg1	mice					1207:1210	the SND-operated mice	1190:1210	the SND-operated mice	1190:1210	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	4	6	theme	gut	687:689	arg1	microbiota					691:700	gut microbiota	687:700	gut microbiota in adult mice after administration of lipopolysaccharide (LPS)	687:763	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	0	7	theme	brain-spleen	194:205	arg1	axis					207:210	brain-spleen axis	194:210	brain-spleen axis	194:210	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	5	8	theme	Iba1	899:902	arg1	expression					885:894	increased expression	875:894	increased expression of Iba1 (ionized calcium-binding adapter molecule 1)	875:947	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	8	theme	Iba1	899:902	arg1	splenomegaly					861:872	splenomegaly	861:872	splenomegaly	861:872	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	8	theme	Iba1	899:902	arg1	phenotype					827:835	depression-like phenotype	811:835	depression-like phenotype	811:835	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	8	theme	Iba1	899:902	arg1	inflammation					847:858	systemic inflammation	838:858	systemic inflammation	838:858	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	8	theme	Iba1	899:902	arg1	expression					963:972	decreased expression	953:972	decreased expression of postsynaptic density protein-95 (PSD-95)	953:1016	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	0	9	theme	systemic	66:73	arg1	inflammation					75:86	systemic inflammation	66:86	systemic inflammation	66:86	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	4	10	from	composition	672:682	arg1	mice					711:714	adult mice	705:714	adult mice	705:714	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	5	11	theme	ionized	905:911	arg1	molecule					937:944	ionized calcium-binding adapter molecule 1	905:946	ionized calcium-binding adapter molecule 1	905:946	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	11	theme	ionized	905:911	arg1	Iba1					899:902	Iba1	899:902	Iba1 (ionized calcium-binding adapter molecule 1)	899:947	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	9	12	theme	expression	1637:1646	arg1	abundance					1584:1592	the relative abundance	1571:1592	the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus	1571:1665	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	0	13	theme	gut	117:119	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	0	14	from	inflammation	75:86	arg1	mice					135:138	mice	135:138	mice after administration of lipopolysaccharide	135:181	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	1	15	theme	BACKGROUND	213:222	arg1	evidence					237:244	BACKGROUND Accumulating evidence	213:244	BACKGROUND Accumulating evidence	213:244	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	4	16	theme	lipopolysaccharide	740:757	arg1	administration					722:735	administration	722:735	administration of lipopolysaccharide (LPS)	722:763	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	0	17	from	composition	102:112	arg1	mice					135:138	mice	135:138	mice after administration of lipopolysaccharide	135:181	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	7	18	theme	necrosis	1421:1428	arg1	factor-α					1430:1437	tumor necrosis factor-α	1415:1437	tumor necrosis factor-α in mice	1415:1445	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	9	19	theme	relative	1575:1582	arg1	abundance					1584:1592	the relative abundance	1571:1592	the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus	1571:1665	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	10	20	theme	relative	1743:1750	arg1	abundance					1752:1760	the relative abundance	1739:1760	the relative abundance of several microbiota	1739:1782	In addition, expression of Iba1 in the hippocampus was correlated with the relative abundance of several microbiota.
36037991	7	21	theme	plasma	1274:1279	arg1	levels					1281:1286	LPS-induced increased plasma levels	1252:1286	LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6	1252:1329	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	5	22	theme	adapter	929:935	arg1	molecule					937:944	ionized calcium-binding adapter molecule 1	905:946	ionized calcium-binding adapter molecule 1	905:946	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	22	theme	adapter	929:935	arg1	Iba1					899:902	Iba1	899:902	Iba1 (ionized calcium-binding adapter molecule 1)	899:947	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	4	23	from	phenotype	625:633	arg1	mice					711:714	adult mice	705:714	adult mice	705:714	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	0	24	from	Effects	0:6	arg1	inflammation					75:86	systemic inflammation	66:86	systemic inflammation	66:86	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	0	24	from	Effects	0:6	arg1	composition					102:112	abnormal composition	93:112	abnormal composition	93:112	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	0	24	from	Effects	0:6	arg1	phenotype					55:63	depression-like phenotype	39:63	depression-like phenotype	39:63	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	12	25	theme	microglial	2015:2024	arg1	function					2026:2033	microglial function	2015:2033	microglial function	2015:2033	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	6	26	theme	depression-like	1097:1111	arg1	phenotype					1113:1121	depression-like phenotype	1097:1121	depression-like phenotype	1097:1121	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	4	27	from	inflammation	645:656	arg1	mice					711:714	adult mice	705:714	adult mice	705:714	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	2	28	theme	nerve	417:421	arg1	effects					396:402	anti-inflammatory effects	378:402	anti-inflammatory effects of the vagus nerve which plays a role in depression	378:454	The spleen mediates anti-inflammatory effects of the vagus nerve which plays a role in depression.
36037991	5	29	theme	systemic	838:845	arg1	inflammation					847:858	systemic inflammation	838:858	systemic inflammation	838:858	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	12	30	theme	gut	1987:1989	arg1	microbiota					1991:2000	gut microbiota	1987:2000	gut microbiota	1987:2000	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	5	31	theme	postsynaptic	977:988	arg1	density					990:996	postsynaptic density protein-95	977:1007	postsynaptic density protein-95 (PSD-95)	977:1016	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	31	theme	postsynaptic	977:988	arg1	PSD-95					1010:1015	PSD-95	1010:1015	PSD-95	1010:1015	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	4	32	theme	nerve	578:582	arg1	denervation					584:594	the splenic nerve denervation	566:594	the splenic nerve denervation (SND)	566:600	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	32	theme	nerve	578:582	arg1	SND					597:599	SND	597:599	SND	597:599	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	3	33	theme	spleen	478:483	arg1	nerve					485:489	spleen nerve	478:489	spleen nerve	478:489	However, the role of spleen nerve in inflammation-related depression remains unclear.
36037991	0	34	theme	spleen	11:16	arg1	denervation					24:34	spleen nerve denervation	11:34	spleen nerve denervation	11:34	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	9	35	from	hippocampus	1655:1665	arg1	abundance					1584:1592	the relative abundance	1571:1592	the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus	1571:1665	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	4	36	from	microbiota	691:700	arg1	mice					711:714	adult mice	705:714	adult mice	705:714	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	8	37	theme	significant	1459:1469	arg1	changes					1471:1477	significant changes	1459:1477	significant changes in several microbiota	1459:1499	There were significant changes in several microbiota among the four groups.
36037991	6	38	from	hippocampus	1175:1185	arg1	mice					1207:1210	the SND-operated mice	1190:1210	the SND-operated mice	1190:1210	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	0	39	theme	denervation	24:34	arg1	Effects					0:6	Effects	0:6	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.	0:211	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	1	40	theme	axis	317:320	arg1	role					259:262	the role	255:262	the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression	255:355	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	10	41	theme	microbiota	1773:1782	arg1	abundance					1752:1760	the relative abundance	1739:1760	the relative abundance of several microbiota	1739:1782	In addition, expression of Iba1 in the hippocampus was correlated with the relative abundance of several microbiota.
36037991	4	42	from	effects	555:561	arg1	composition					672:682	abnormal composition	663:682	abnormal composition	663:682	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	42	from	effects	555:561	arg1	phenotype					625:633	the depression-like phenotype	605:633	the depression-like phenotype	605:633	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	42	from	effects	555:561	arg1	inflammation					645:656	systemic inflammation	636:656	systemic inflammation	636:656	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	1	43	theme	inflammation-related	325:344	arg1	depression					346:355	inflammation-related depression	325:355	inflammation-related depression	325:355	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	12	44	theme	inflammation-related	1903:1922	arg1	depression					1924:1933	inflammation-related depression	1903:1933	inflammation-related depression	1903:1933	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	12	44	theme	inflammation-related	1903:1922	arg1	activation					1947:1956	microglial activation	1936:1956	microglial activation in the hippocampus	1936:1975	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	1	45	theme	Accumulating	224:235	arg1	evidence					237:244	BACKGROUND Accumulating evidence	213:244	BACKGROUND Accumulating evidence	213:244	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	12	46	theme	microglial	1936:1945	arg1	depression					1924:1933	inflammation-related depression	1903:1933	inflammation-related depression	1903:1933	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	12	46	theme	microglial	1936:1945	arg1	activation					1947:1956	microglial activation	1936:1956	microglial activation in the hippocampus	1936:1975	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	12	47	theme	splenic	1873:1879	arg1	nerve					1881:1885	the splenic nerve	1869:1885	the splenic nerve	1869:1885	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	9	48	theme	several	1597:1603	arg1	PSD-95					1629:1634	PSD-95	1629:1634	PSD-95	1629:1634	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	9	48	theme	several	1597:1603	arg1	microbiota					1605:1614	several microbiota	1597:1614	several microbiota	1597:1614	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	11	49	theme	LIMITATIONS	1785:1795	arg1	mechanisms					1806:1815	LIMITATIONS Detailed mechanisms	1785:1815	LIMITATIONS Detailed mechanisms	1785:1815	LIMITATIONS Detailed mechanisms are unclear.
36037991	0	50	dep	Effects	0:6	arg1	role					186:189	A role	184:189	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.	0:211	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	6	51	theme	abnormal	1128:1135	arg1	expressions					1137:1147	abnormal expressions	1128:1147	abnormal expressions of Iba1 and PSD-95	1128:1166	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	4	52	theme	systemic	636:643	arg1	inflammation					645:656	systemic inflammation	636:656	systemic inflammation	636:656	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	9	53	from	abundance	1584:1592	arg1	hippocampus					1655:1665	the hippocampus	1651:1665	the hippocampus	1651:1665	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	1	54	theme	brain-spleen	267:278	arg1	axis					280:283	brain-spleen axis	267:283	brain-spleen axis as well as brain-gut-microbiota axis	267:320	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	10	55	from	expression	1681:1690	arg1	hippocampus					1707:1717	the hippocampus	1703:1717	the hippocampus	1703:1717	In addition, expression of Iba1 in the hippocampus was correlated with the relative abundance of several microbiota.
36037991	0	56	theme	axis	207:210	arg1	role					186:189	A role	184:189	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.	0:211	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	8	57	from	changes	1471:1477	arg1	microbiota					1490:1499	several microbiota	1482:1499	several microbiota	1482:1499	There were significant changes in several microbiota among the four groups.
36037991	0	58	theme	depression-like	39:53	arg1	phenotype					55:63	depression-like phenotype	39:63	depression-like phenotype	39:63	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	7	59	theme	LPS-induced	1252:1262	arg1	levels					1281:1286	LPS-induced increased plasma levels	1252:1286	LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6	1252:1329	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	5	60	from	hippocampus	1025:1035	arg1	mice					1058:1061	the sham-operated mice	1040:1061	the sham-operated mice	1040:1061	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	12	61	theme	gut-microbiota-brain	2052:2071	arg1	axis					2073:2076	gut-microbiota-brain axis	2052:2076	gut-microbiota-brain axis	2052:2076	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	4	62	theme	microbiota	691:700	arg1	composition					672:682	abnormal composition	663:682	abnormal composition	663:682	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	62	theme	microbiota	691:700	arg1	phenotype					625:633	the depression-like phenotype	605:633	the depression-like phenotype	605:633	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	62	theme	microbiota	691:700	arg1	inflammation					645:656	systemic inflammation	636:656	systemic inflammation	636:656	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	0	63	theme	microbiota	121:130	arg1	inflammation					75:86	systemic inflammation	66:86	systemic inflammation	66:86	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	0	63	theme	microbiota	121:130	arg1	composition					102:112	abnormal composition	93:112	abnormal composition	93:112	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	0	63	theme	microbiota	121:130	arg1	phenotype					55:63	depression-like phenotype	39:63	depression-like phenotype	39:63	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	10	64	theme	Iba1	1695:1698	arg1	expression					1681:1690	expression	1681:1690	expression of Iba1 in the hippocampus	1681:1717	In addition, expression of Iba1 in the hippocampus was correlated with the relative abundance of several microbiota.
36037991	1	65	from	role	259:262	arg1	depression					346:355	inflammation-related depression	325:355	inflammation-related depression	325:355	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	5	66	theme	increased	875:883	arg1	expression					885:894	increased expression	875:894	increased expression of Iba1 (ionized calcium-binding adapter molecule 1)	875:947	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	12	67	from	activation	1947:1956	arg1	hippocampus					1965:1975	the hippocampus	1961:1975	the hippocampus	1961:1975	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	4	68	theme	abnormal	663:670	arg1	composition					672:682	abnormal composition	663:682	abnormal composition	663:682	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	7	69	theme	increased	1264:1272	arg1	levels					1281:1286	LPS-induced increased plasma levels	1252:1286	LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6	1252:1329	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	12	70	dep	CONCLUSIONS	1830:1840	arg1	suggest					1856:1862	suggest	1856:1862	suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis	1856:2076	CONCLUSIONS These results suggest that the splenic nerve plays a role in inflammation-related depression, microglial activation in the hippocampus, and that gut microbiota may regulate microglial function in the brain via gut-microbiota-brain axis.
36037991	0	71	theme	lipopolysaccharide	164:181	arg1	administration					146:159	administration	146:159	administration of lipopolysaccharide	146:181	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	5	72	theme	decreased	953:961	arg1	expression					963:972	decreased expression	953:972	decreased expression of postsynaptic density protein-95 (PSD-95)	953:1016	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	7	73	theme	factor-α	1430:1437	arg1	levels					1405:1410	plasma levels	1398:1410	plasma levels of tumor necrosis factor-α in mice	1398:1445	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	6	74	theme	Iba1	1152:1155	arg1	expressions					1137:1147	abnormal expressions	1128:1147	abnormal expressions of Iba1 and PSD-95	1128:1166	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	6	74	theme	Iba1	1152:1155	arg1	phenotype					1113:1121	depression-like phenotype	1097:1121	depression-like phenotype	1097:1121	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	5	75	theme	RESULTS	780:786	arg1	LPS					788:790	RESULTS LPS	780:790	RESULTS LPS (0.5 mg/kg)	780:802	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	75	theme	RESULTS	780:786	arg1	0.5 mg/kg					793:801	0.5 mg/kg	793:801	0.5 mg/kg	793:801	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	76	theme	calcium-binding	913:927	arg1	molecule					937:944	ionized calcium-binding adapter molecule 1	905:946	ionized calcium-binding adapter molecule 1	905:946	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	76	theme	calcium-binding	913:927	arg1	Iba1					899:902	Iba1	899:902	Iba1 (ionized calcium-binding adapter molecule 1)	899:947	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	9	77	from	expression	1637:1646	arg1	hippocampus					1655:1665	the hippocampus	1651:1665	the hippocampus	1651:1665	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	7	78	theme	tumor	1415:1419	arg1	factor-α					1430:1437	tumor necrosis factor-α	1415:1437	tumor necrosis factor-α in mice	1415:1445	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	4	79	theme	adult	705:709	arg1	mice					711:714	adult mice	705:714	adult mice	705:714	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	9	80	theme	microbiota	1605:1614	arg1	expression					1637:1646	several microbiota and Iba1 (or PSD-95) expression	1597:1646	several microbiota and Iba1 (or PSD-95) expression in the hippocampus	1597:1665	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	0	81	theme	abnormal	93:100	arg1	composition					102:112	abnormal composition	93:112	abnormal composition	93:112	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	9	82	theme	Iba1	1620:1623	arg1	expression					1637:1646	several microbiota and Iba1 (or PSD-95) expression	1597:1646	several microbiota and Iba1 (or PSD-95) expression in the hippocampus	1597:1665	Interestingly, there were correlations between the relative abundance of several microbiota and Iba1 (or PSD-95) expression in the hippocampus.
36037991	0	83	from	phenotype	55:63	arg1	mice					135:138	mice	135:138	mice after administration of lipopolysaccharide	135:181	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	3	84	theme	inflammation-related	494:513	arg1	depression					515:524	inflammation-related depression	494:524	inflammation-related depression	494:524	However, the role of spleen nerve in inflammation-related depression remains unclear.
36037991	7	85	theme	plasma	1398:1403	arg1	levels					1405:1410	plasma levels	1398:1410	plasma levels of tumor necrosis factor-α in mice	1398:1445	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	7	86	from	factor-α	1430:1437	arg1	mice					1442:1445	mice	1442:1445	mice	1442:1445	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	4	87	theme	denervation	584:594	arg1	effects					555:561	The effects	551:561	The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS)	551:763	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	2	88	theme	vagus	411:415	arg1	nerve					417:421	the vagus nerve	407:421	the vagus nerve which plays a role in depression	407:454	The spleen mediates anti-inflammatory effects of the vagus nerve which plays a role in depression.
36037991	0	89	theme	nerve	18:22	arg1	denervation					24:34	spleen nerve denervation	11:34	spleen nerve denervation	11:34	Effects of spleen nerve denervation on depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in mice after administration of lipopolysaccharide: A role of brain-spleen axis.
36037991	5	90	theme	density	990:996	arg1	expression					885:894	increased expression	875:894	increased expression of Iba1 (ionized calcium-binding adapter molecule 1)	875:947	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	90	theme	density	990:996	arg1	splenomegaly					861:872	splenomegaly	861:872	splenomegaly	861:872	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	90	theme	density	990:996	arg1	phenotype					827:835	depression-like phenotype	811:835	depression-like phenotype	811:835	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	90	theme	density	990:996	arg1	inflammation					847:858	systemic inflammation	838:858	systemic inflammation	838:858	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	5	90	theme	density	990:996	arg1	expression					963:972	decreased expression	953:972	decreased expression of postsynaptic density protein-95 (PSD-95)	953:1016	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	7	91	from	mice	1442:1445	arg1	levels					1405:1410	plasma levels	1398:1410	plasma levels of tumor necrosis factor-α in mice	1398:1445	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	7	92	theme	LPS-induced	1359:1369	arg1	splenomegaly					1371:1382	LPS-induced splenomegaly	1359:1382	LPS-induced splenomegaly	1359:1382	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	3	93	theme	nerve	485:489	arg1	role					470:473	the role	466:473	the role of spleen nerve in inflammation-related depression	466:524	However, the role of spleen nerve in inflammation-related depression remains unclear.
36037991	1	94	theme	brain-gut-microbiota	296:315	arg1	axis					317:320	brain-gut-microbiota axis	296:320	brain-spleen axis as well as brain-gut-microbiota axis	267:320	BACKGROUND Accumulating evidence suggests the role of brain-spleen axis as well as brain-gut-microbiota axis in inflammation-related depression.
36037991	5	95	theme	sham-operated	1044:1056	arg1	mice					1058:1061	the sham-operated mice	1040:1061	the sham-operated mice	1040:1061	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	3	96	from	role	470:473	arg1	depression					515:524	inflammation-related depression	494:524	inflammation-related depression	494:524	However, the role of spleen nerve in inflammation-related depression remains unclear.
36037991	5	97	theme	depression-like	811:825	arg1	phenotype					827:835	depression-like phenotype	811:835	depression-like phenotype	811:835	RESULTS LPS (0.5 mg/kg) caused depression-like phenotype, systemic inflammation, splenomegaly, increased expression of Iba1 (ionized calcium-binding adapter molecule 1) and decreased expression of postsynaptic density protein-95 (PSD-95) in the hippocampus in the sham-operated mice.
36037991	4	98	dep	METHODS	543:549	arg1	examined					770:777	examined	770:777	were examined	765:777	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	6	99	theme	PSD-95	1161:1166	arg1	expressions					1137:1147	abnormal expressions	1128:1147	abnormal expressions of Iba1 and PSD-95	1128:1166	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	6	99	theme	PSD-95	1161:1166	arg1	phenotype					1113:1121	depression-like phenotype	1097:1121	depression-like phenotype	1097:1121	In contrast, LPS did not produce depression-like phenotype, and abnormal expressions of Iba1 and PSD-95 in the hippocampus in the SND-operated mice.
36037991	4	100	theme	splenic	570:576	arg1	denervation					584:594	the splenic nerve denervation	566:594	the splenic nerve denervation (SND)	566:600	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	4	100	theme	splenic	570:576	arg1	SND					597:599	SND	597:599	SND	597:599	METHODS The effects of the splenic nerve denervation (SND) in the depression-like phenotype, systemic inflammation, and abnormal composition of gut microbiota in adult mice after administration of lipopolysaccharide (LPS) were examined.
36037991	11	101	theme	Detailed	1797:1804	arg1	mechanisms					1806:1815	LIMITATIONS Detailed mechanisms	1785:1815	LIMITATIONS Detailed mechanisms	1785:1815	LIMITATIONS Detailed mechanisms are unclear.
36037991	10	102	theme	several	1765:1771	arg1	microbiota					1773:1782	several microbiota	1765:1782	several microbiota	1765:1782	In addition, expression of Iba1 in the hippocampus was correlated with the relative abundance of several microbiota.
36037991	7	103	theme	interleukin-6	1317:1329	arg1	levels					1281:1286	LPS-induced increased plasma levels	1252:1286	LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6	1252:1329	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	8	104	theme	several	1482:1488	arg1	microbiota					1490:1499	several microbiota	1482:1499	several microbiota	1482:1499	There were significant changes in several microbiota among the four groups.
36037991	7	105	theme	cytokine	1308:1315	arg1	interleukin-6					1317:1329	pro-inflammatory cytokine interleukin-6	1291:1329	pro-inflammatory cytokine interleukin-6	1291:1329	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
36037991	7	106	theme	pro-inflammatory	1291:1306	arg1	interleukin-6					1317:1329	pro-inflammatory cytokine interleukin-6	1291:1329	pro-inflammatory cytokine interleukin-6	1291:1329	Furthermore, SND significantly blocked LPS-induced increased plasma levels of pro-inflammatory cytokine interleukin-6 although SND did not affect LPS-induced splenomegaly and increased plasma levels of tumor necrosis factor-α in mice.
34740281	0	0	theme	photodissociation	71:87	arg1	spectrometry					94:105	photodissociation mass spectrometry	71:105	photodissociation mass spectrometry	71:105	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.
34740281	2	1	theme	rich	467:470	arg1	patterns					499:506	rich and valuable fragmentation patterns	467:506	rich and valuable fragmentation patterns	467:506	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	5	2	from	irradiated	877:886	arg1	cell					948:951	the FTICR cell	938:951	the FTICR cell	938:951	The complex ions were further isolated and irradiated by IR and UV lasers separately or continuously in the FTICR cell.
34740281	1	3	theme	spectrometry	263:274	arg1	methods					289:295	mass spectrometry (MS)-related methods	258:295	mass spectrometry (MS)-related methods	258:295	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	0	4	theme	UV	68:69	arg1	combination					46:56	a combination	44:56	a combination of IR and UV photodissociation mass spectrometry	44:105	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.
34740281	5	5	dep	IR	891:892	arg1	lasers					901:906	lasers	901:906	lasers	901:906	The complex ions were further isolated and irradiated by IR and UV lasers separately or continuously in the FTICR cell.
34740281	10	6	dep	simpler	1775:1781	arg1	faster					1784:1789	faster	1784:1789	faster	1784:1789	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	10	6	dep	simpler	1775:1781	arg1	flexible					1797:1804	flexible	1797:1804	flexible	1797:1804	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	6	7	theme	isomers	1089:1095	arg1	identification					1059:1072	a clear identification	1051:1072	a clear identification of all the ten isomers	1051:1095	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	2	8	theme	ultraviolet	342:352	arg1	lasers					359:364	infrared (IR) and ultraviolet (UV) lasers	324:364	infrared (IR) and ultraviolet (UV) lasers	324:364	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	2	9	theme	glycan	512:517	arg1	analysis					519:526	glycan analysis	512:526	glycan analysis	512:526	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	7	10	theme	full	1340:1343	arg1	discrimination					1345:1358	the full discrimination	1336:1358	the full discrimination	1336:1358	The double-beam method simplifies the experiment by introducing the two lasers sequentially in one experiment, providing richer fragmentation patterns and making the full discrimination easier.
34740281	6	11	theme	fragmentation	1150:1162	arg1	patterns					1164:1171	their fragmentation patterns	1144:1171	their fragmentation patterns	1144:1171	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	9	12	theme	compositional	1593:1605	arg1	isomers					1654:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	The double-beam photodissociation method described here distinguished compositional, configurational and connectivity disaccharide isomers successfully.
34740281	2	13	theme	photodissociation	302:318	arg1	MS					320:321	photodissociation MS	302:321	photodissociation MS	302:321	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	6	14	theme	fragment	997:1004	arg1	spectra					1006:1012	the two complementary fragment spectra	975:1012	the two complementary fragment spectra generated from the IR and UV lasers	975:1048	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	0	15	theme	mass	89:92	arg1	spectrometry					94:105	photodissociation mass spectrometry	71:105	photodissociation mass spectrometry	71:105	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.
34740281	4	16	theme	complex	756:762	arg1	ions					764:767	complex ions	756:767	complex ions	756:767	3,5-Diiodo-L-tyrosine was selected as the assistant molecule to form complex ions with ten isomeric disaccharides through electrospray ionization.
34740281	10	17	theme	accumulation	1699:1710	arg1	method					1712:1717	the data accumulation method	1690:1717	the data accumulation method based on separate IR and UV experiments	1690:1757	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	2	18	dep	ultraviolet	342:352	arg1	UV					355:356	UV	355:356	UV	355:356	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	6	19	theme	complementary	983:995	arg1	spectra					1006:1012	the two complementary fragment spectra	975:1012	the two complementary fragment spectra generated from the IR and UV lasers	975:1048	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	7	20	theme	double-beam	1178:1188	arg1	method					1190:1195	The double-beam method	1174:1195	The double-beam method	1174:1195	The double-beam method simplifies the experiment by introducing the two lasers sequentially in one experiment, providing richer fragmentation patterns and making the full discrimination easier.
34740281	8	21	dep	CONCLUSIONS	1368:1378	arg1	demonstrates					1391:1402	demonstrates	1391:1402	demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers	1391:1520	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	9	22	theme	configurational	1608:1622	arg1	isomers					1654:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	The double-beam photodissociation method described here distinguished compositional, configurational and connectivity disaccharide isomers successfully.
34740281	1	23	theme	structural	170:179	arg1	complexity					181:190	their structural complexity	164:190	their structural complexity	164:190	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	2	24	theme	fragmentation	485:497	arg1	patterns					499:506	rich and valuable fragmentation patterns	467:506	rich and valuable fragmentation patterns	467:506	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	10	25	theme	data	1694:1697	arg1	method					1712:1717	the data accumulation method	1690:1717	the data accumulation method based on separate IR and UV experiments	1690:1757	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	5	26	theme	FTICR	942:946	arg1	cell					948:951	the FTICR cell	938:951	the FTICR cell	938:951	The complex ions were further isolated and irradiated by IR and UV lasers separately or continuously in the FTICR cell.
34740281	8	27	theme	IR	1443:1444	arg1	combination					1428:1438	the combination	1424:1438	the combination of IR and UV photodissociation MS	1424:1472	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	7	28	theme	richer	1295:1300	arg1	patterns					1316:1323	richer fragmentation patterns	1295:1323	richer fragmentation patterns	1295:1323	The double-beam method simplifies the experiment by introducing the two lasers sequentially in one experiment, providing richer fragmentation patterns and making the full discrimination easier.
34740281	2	29	theme	infrared	324:331	arg1	lasers					359:364	infrared (IR) and ultraviolet (UV) lasers	324:364	infrared (IR) and ultraviolet (UV) lasers	324:364	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	10	30	theme	separate	1728:1735	arg1	IR					1737:1738	separate IR	1728:1738	separate IR	1728:1738	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	6	31	theme	IR	1033:1034	arg1	lasers					1043:1048	the IR and UV lasers	1029:1048	the IR and UV lasers	1029:1048	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	1	32	theme	-related	280:287	arg1	methods					289:295	mass spectrometry (MS)-related methods	258:295	mass spectrometry (MS)-related methods	258:295	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	8	33	theme	glycan	1507:1512	arg1	isomers					1514:1520	diverse glycan isomers	1499:1520	diverse glycan isomers	1499:1520	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	3	34	theme	ion	563:565	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	9	35	theme	disaccharide	1641:1652	arg1	isomers					1654:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	The double-beam photodissociation method described here distinguished compositional, configurational and connectivity disaccharide isomers successfully.
34740281	6	36	theme	clear	1053:1057	arg1	identification					1059:1072	a clear identification	1051:1072	a clear identification of all the ten isomers	1051:1095	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	2	37	theme	effective	414:422	arg1	combination					424:434	an effective combination	411:434	an effective combination of the two methods	411:453	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	4	38	theme	assistant	729:737	arg1	3,5-Diiodo-L-tyrosine					687:707	3,5-Diiodo-L-tyrosine	687:707	3,5-Diiodo-L-tyrosine	687:707	3,5-Diiodo-L-tyrosine was selected as the assistant molecule to form complex ions with ten isomeric disaccharides through electrospray ionization.
34740281	4	38	theme	assistant	729:737	arg1	molecule					739:746	the assistant molecule	725:746	the assistant molecule	725:746	3,5-Diiodo-L-tyrosine was selected as the assistant molecule to form complex ions with ten isomeric disaccharides through electrospray ionization.
34740281	8	39	theme	combination	1428:1438	arg1	capabilities					1408:1419	the capabilities	1404:1419	the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers	1404:1520	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	3	40	theme	7.0 T	539:543	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	10	41	theme	UV	1744:1745	arg1	experiments					1747:1757	UV experiments	1744:1757	UV experiments	1744:1757	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	0	42	theme	disaccharide	19:30	arg1	isomers					32:38	disaccharide isomers	19:38	disaccharide isomers	19:38	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.
34740281	3	43	dep	METHODS	529:535	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	3	44	theme	double-beam	629:639	arg1	system					647:652	a double-beam laser system	627:652	a double-beam laser system	627:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	0	45	dep	IR	61:62	arg1	spectrometry					94:105	photodissociation mass spectrometry	71:105	photodissociation mass spectrometry	71:105	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.
34740281	8	46	theme	photodissociation	1453:1469	arg1	MS					1471:1472	photodissociation MS	1453:1472	photodissociation MS	1453:1472	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	7	47	theme	fragmentation	1302:1314	arg1	patterns					1316:1323	richer fragmentation patterns	1295:1323	richer fragmentation patterns	1295:1323	The double-beam method simplifies the experiment by introducing the two lasers sequentially in one experiment, providing richer fragmentation patterns and making the full discrimination easier.
34740281	9	48	theme	connectivity	1628:1639	arg1	isomers					1654:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	compositional, configurational and connectivity disaccharide isomers	1593:1660	The double-beam photodissociation method described here distinguished compositional, configurational and connectivity disaccharide isomers successfully.
34740281	3	49	theme	laser	641:645	arg1	system					647:652	a double-beam laser system	627:652	a double-beam laser system	627:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	3	50	dep	spectrometer	600:611	arg1	transform					553:561	transform	553:561	transform	553:561	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	2	51	theme	methods	447:453	arg1	combination					424:434	an effective combination	411:434	an effective combination of the two methods	411:453	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	0	52	theme	isomers	32:38	arg1	Differentiation					0:14	Differentiation	0:14	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.	0:106	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.
34740281	2	53	theme	fragment	393:400	arg1	ions					402:405	complementary fragment ions	379:405	complementary fragment ions	379:405	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	8	54	dep	IR	1443:1444	arg1	MS					1471:1472	photodissociation MS	1453:1472	photodissociation MS	1453:1472	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	5	55	theme	complex	838:844	arg1	ions					846:849	The complex ions	834:849	The complex ions	834:849	The complex ions were further isolated and irradiated by IR and UV lasers separately or continuously in the FTICR cell.
34740281	3	56	theme	cyclotron	567:575	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	9	57	theme	photodissociation	1539:1555	arg1	method					1557:1562	The double-beam photodissociation method	1523:1562	The double-beam photodissociation method described here	1523:1577	The double-beam photodissociation method described here distinguished compositional, configurational and connectivity disaccharide isomers successfully.
34740281	2	58	theme	complementary	379:391	arg1	ions					402:405	complementary fragment ions	379:405	complementary fragment ions	379:405	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	9	59	theme	double-beam	1527:1537	arg1	method					1557:1562	The double-beam photodissociation method	1523:1562	The double-beam photodissociation method described here	1523:1577	The double-beam photodissociation method described here distinguished compositional, configurational and connectivity disaccharide isomers successfully.
34740281	8	60	theme	diverse	1499:1505	arg1	isomers					1514:1520	diverse glycan isomers	1499:1520	diverse glycan isomers	1499:1520	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	3	61	theme	resonance	577:585	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	6	62	dep	binary	1122:1127	arg1	codes					1129:1133	codes	1129:1133	codes	1129:1133	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	10	63	theme	richer	1832:1837	arg1	patterns					1853:1860	richer fragmentation patterns	1832:1860	richer fragmentation patterns	1832:1860	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	8	64	theme	UV	1450:1451	arg1	combination					1428:1438	the combination	1424:1438	the combination of IR and UV photodissociation MS	1424:1472	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	7	65	dep	simplifies	1197:1206	arg1	making					1329:1334	making	1329:1334	making the full discrimination easier	1329:1365	The double-beam method simplifies the experiment by introducing the two lasers sequentially in one experiment, providing richer fragmentation patterns and making the full discrimination easier.
34740281	7	65	dep	simplifies	1197:1206	arg1	providing					1285:1293	providing	1285:1293	providing richer fragmentation patterns	1285:1323	The double-beam method simplifies the experiment by introducing the two lasers sequentially in one experiment, providing richer fragmentation patterns and making the full discrimination easier.
34740281	3	66	theme	FTICR	588:592	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	2	67	dep	infrared	324:331	arg1	IR					334:335	IR	334:335	IR	334:335	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	10	68	theme	fragmentation	1839:1851	arg1	patterns					1853:1860	richer fragmentation patterns	1832:1860	richer fragmentation patterns	1832:1860	Compared with the data accumulation method based on separate IR and UV experiments, this method is simpler, faster, more flexible and also characterized by richer fragmentation patterns.
34740281	1	69	theme	recent	235:240	arg1	developments					242:253	recent developments	235:253	recent developments in mass spectrometry (MS)-related methods	235:295	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	1	70	from	developments	242:253	arg1	methods					289:295	mass spectrometry (MS)-related methods	258:295	mass spectrometry (MS)-related methods	258:295	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	8	71	theme	isomers	1514:1520	arg1	identification					1481:1494	the identification	1477:1494	the identification of diverse glycan isomers	1477:1520	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	4	72	theme	isomeric	778:785	arg1	disaccharides					787:799	ten isomeric disaccharides	774:799	ten isomeric disaccharides	774:799	3,5-Diiodo-L-tyrosine was selected as the assistant molecule to form complex ions with ten isomeric disaccharides through electrospray ionization.
34740281	1	73	theme	glycan	135:140	arg1	identification					142:155	glycan identification	135:155	glycan identification	135:155	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	3	74	theme	Fourier	545:551	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	2	75	theme	valuable	476:483	arg1	patterns					499:506	rich and valuable fragmentation patterns	467:506	rich and valuable fragmentation patterns	467:506	For photodissociation MS, infrared (IR) and ultraviolet (UV) lasers can generate complementary fragment ions, so an effective combination of the two methods may provide rich and valuable fragmentation patterns for glycan analysis.
34740281	6	76	theme	UV	1040:1041	arg1	lasers					1043:1048	the IR and UV lasers	1029:1048	the IR and UV lasers	1029:1048	RESULTS By combining the two complementary fragment spectra generated from the IR and UV lasers, a clear identification of all the ten isomers was achieved using their binary codes based on their fragmentation patterns.
34740281	4	77	theme	electrospray	809:820	arg1	ionization					822:831	electrospray ionization	809:831	electrospray ionization	809:831	3,5-Diiodo-L-tyrosine was selected as the assistant molecule to form complex ions with ten isomeric disaccharides through electrospray ionization.
34740281	1	78	theme	identification	142:155	arg1	challenge					122:130	The challenge	118:130	The challenge of glycan identification due to their structural complexity and diversity	118:204	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	5	79	from	cell	948:951	arg1	irradiated					877:886	irradiated	877:886	irradiated	877:886	The complex ions were further isolated and irradiated by IR and UV lasers separately or continuously in the FTICR cell.
34740281	0	80	theme	IR	61:62	arg1	combination					46:56	a combination	44:56	a combination of IR and UV photodissociation mass spectrometry	44:105	Differentiation of disaccharide isomers via a combination of IR and UV photodissociation mass spectrometry.
34740281	3	81	theme	mass	595:598	arg1	spectrometer					600:611	A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer	537:611	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system	529:652	METHODS A 7.0 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a double-beam laser system was applied for the experiments.
34740281	1	82	dep	RATIONALE	108:116	arg1	profited					210:217	profited	210:217	has profited enormously from recent developments in mass spectrometry (MS)-related methods	206:295	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	8	83	from	capabilities	1408:1419	arg1	identification					1481:1494	the identification	1477:1494	the identification of diverse glycan isomers	1477:1520	CONCLUSIONS This study demonstrates the capabilities of the combination of IR and UV photodissociation MS in the identification of diverse glycan isomers.
34740281	1	84	theme	mass	258:261	arg1	MS					277:278	MS	277:278	MS	277:278	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
34740281	1	84	theme	mass	258:261	arg1	spectrometry					263:274	mass spectrometry	258:274	mass spectrometry (MS)-related methods	258:295	RATIONALE The challenge of glycan identification due to their structural complexity and diversity has profited enormously from recent developments in mass spectrometry (MS)-related methods.
37299439	4	0	theme	sodium	539:544	arg1	sulfate					546:552	dextran sodium sulfate	531:552	dextran sodium sulfate (DSS)	531:558	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	4	0	theme	sodium	539:544	arg1	DSS					555:557	DSS	555:557	DSS	555:557	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	2	1	theme	bowel	333:337	arg1	disease					339:345	an inflammatory bowel disease	317:345	an inflammatory bowel disease (IBD)	317:351	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	2	1	theme	bowel	333:337	arg1	IBD					348:350	IBD	348:350	IBD	348:350	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	5	2	theme	experimental	969:980	arg1	period					982:987	the entire 14-day experimental period	951:987	the entire 14-day experimental period	951:987	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	12	3	theme	serum	1916:1920	arg1	indicators					1934:1943	serum biochemical indicators	1916:1943	serum biochemical indicators	1916:1943	Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations.
37299439	13	4	theme	inflammatory	2115:2126	arg1	cytokines					2128:2136	inflammatory cytokines	2115:2136	inflammatory cytokines	2115:2136	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	4	5	from	colitis	609:615	arg1	mice					620:623	mice	620:623	mice	620:623	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	11	6	theme	genus	1808:1812	arg1	level					1814:1818	the genus level	1804:1818	the genus level	1804:1818	Moreover, OKG enhanced the abundance of Firmicutes and decreased that of Bacteriodetes at the phylum level and particularly enhanced the abundance of Alistipes and reduced that of Parabacterioides at the genus level.
37299439	5	7	theme	14-day	962:967	arg1	period					982:987	the entire 14-day experimental period	951:987	the entire 14-day experimental period	951:987	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	1	8	theme	nutritional	172:182	arg1	compound					184:191	a nutritional compound	170:191	a nutritional compound	170:191	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	8	theme	nutritional	172:182	arg1	α-ketoglutarate					147:161	Ornithine α-ketoglutarate	137:161	Ornithine α-ketoglutarate (OKG)	137:167	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	6	9	theme	1	1020:1020	arg1	%					1021:1021	%	1021:1021	%	1021:1021	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	4	10	theme	preventive	569:578	arg1	effect					580:585	the preventive effect	565:585	the preventive effect of OKG on DSS-induced colitis in mice	565:623	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	5	11	dep	LOKG	806:809	arg1	%					815:815	0.5%	812:815	0.5%	812:815	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	0	12	from	Cytokines	84:92	arg1	Sodium					113:118	Dextran Sulfate Sodium	97:118	Dextran Sulfate Sodium	97:118	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	9	13	theme	weight	1476:1481	arg1	loss					1483:1486	weight loss	1476:1486	weight loss	1476:1486	The results revealed that OKG alleviated weight loss and reversed the increases in colonic histological damage induced by DSS.
37299439	1	14	from	effects	258:264	arg1	animals					280:286	animals	280:286	animals	280:286	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	14	from	effects	258:264	arg1	humans					269:274	humans	269:274	humans	269:274	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	15	theme	acid	206:209	arg1	salt					211:214	an amino acid salt	197:214	an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals	197:286	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	15	theme	acid	206:209	arg1	α-ketoglutarate					147:161	Ornithine α-ketoglutarate	137:161	Ornithine α-ketoglutarate (OKG)	137:167	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	13	16	theme	DSS-induced	2021:2031	arg1	colitis					2033:2039	DSS-induced colitis	2021:2039	DSS-induced colitis in mice	2021:2047	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	8	17	theme	%	1410:1410	arg1	DSS					1412:1414	4% DSS	1409:1414	4% DSS to induce colitis	1409:1432	During days 14 to 21, the DSS mice were administered 4% DSS to induce colitis.
37299439	13	18	from	secretion	2102:2110	arg1	serum					2141:2145	serum	2141:2145	serum	2141:2145	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	4	19	theme	serum	658:662	arg1	cytokines					677:685	serum inflammatory cytokines	658:685	serum inflammatory cytokines	658:685	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	6	20	theme	serum	1066:1070	arg1	hormone					1079:1085	serum growth hormone	1066:1085	serum growth hormone (GH)	1066:1090	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	6	20	theme	serum	1066:1070	arg1	GH					1088:1089	GH	1088:1089	GH	1088:1089	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	13	21	from	colitis	2033:2039	arg1	mice					2044:2047	mice	2044:2047	mice	2044:2047	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	0	22	theme	Serum	65:69	arg1	Cytokines					84:92	Serum Inflammatory Cytokines	65:92	Serum Inflammatory Cytokines	65:92	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	5	23	theme	control	758:764	arg1	group					766:770	a control group	756:770	a control group	756:770	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	5	24	theme	OKG	801:803	arg1	dose					793:796	a low dose	787:796	a low dose of OKG (LOKG: 0.5%)	787:816	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	7	25	used	used	1231:1234	arg2	factors					1347:1353	the main factors	1338:1353	the main factors	1338:1353	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	7	25	used	used	1231:1234	arg2	design					1220:1225	a 2 × 2 factor design	1205:1225	a 2 × 2 factor design	1205:1225	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	9	26	theme	histological	1526:1537	arg1	damage					1539:1544	colonic histological damage	1518:1544	colonic histological damage induced by DSS	1518:1559	The results revealed that OKG alleviated weight loss and reversed the increases in colonic histological damage induced by DSS.
37299439	6	27	theme	alkaline	1108:1115	arg1	phosphatase					1117:1127	alkaline phosphatase	1108:1127	alkaline phosphatase (ALP)	1108:1133	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	6	27	theme	alkaline	1108:1115	arg1	ALP					1130:1132	ALP	1130:1132	ALP	1130:1132	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	0	28	from	Effect	0:5	arg1	Cytokines					84:92	Serum Inflammatory Cytokines	65:92	Serum Inflammatory Cytokines	65:92	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	0	28	from	Effect	0:5	arg1	Microbiota					50:59	Intestinal Microbiota	39:59	Intestinal Microbiota	39:59	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	0	29	theme	Dextran	97:103	arg1	Sodium					113:118	Dextran Sulfate Sodium	97:118	Dextran Sulfate Sodium	97:118	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	5	30	theme	OKG	899:901	arg1	dose					891:894	a high dose	884:894	a high dose of OKG (HOKG: 1.5%)	884:914	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	7	31	with	mice	1254:1257	arg1	challenge					1309:1317	challenge	1309:1317	challenge (4% DSS or not)	1309:1333	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	7	31	with	mice	1254:1257	arg1	diet					1265:1268	diet	1265:1268	diet (a standard diet or a 1% OKG diet)	1265:1303	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	5	32	theme	medium	835:840	arg1	dose					842:845	a medium dose	833:845	a medium dose of OKG (MOKG: 1%)	833:863	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	7	33	theme	OKG	1295:1297	arg1	diet					1299:1302	a 1% OKG diet	1290:1302	a 1% OKG diet	1290:1302	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	7	34	theme	factor	1213:1218	arg1	design					1220:1225	a 2 × 2 factor design	1205:1225	a 2 × 2 factor design	1205:1225	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	7	34	theme	factor	1213:1218	arg1	factors					1347:1353	the main factors	1338:1353	the main factors	1338:1353	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	0	35	from	Microbiota	50:59	arg1	Sodium					113:118	Dextran Sulfate Sodium	97:118	Dextran Sulfate Sodium	97:118	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	13	36	theme	cytokines	2128:2136	arg1	secretion					2102:2110	the secretion	2098:2110	the secretion of inflammatory cytokines in serum	2098:2145	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	13	37	theme	OKG	1992:1994	arg1	supplementation					1996:2010	1% OKG supplementation	1989:2010	1% OKG supplementation	1989:2010	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	7	38	theme	1	1292:1292	arg1	%					1293:1293	%	1293:1293	%	1293:1293	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	4	39	theme	cytokines	677:685	arg1	analysis					646:653	analysis	646:653	analysis of serum inflammatory cytokines and fecal microbiota	646:706	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	4	40	theme	colitis	501:507	arg1	model					486:490	a mouse model	478:490	a mouse model of acute colitis	478:507	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	4	41	theme	fecal	691:695	arg1	microbiota					697:706	fecal microbiota	691:706	fecal microbiota	691:706	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	0	42	theme	Ornithine	10:18	arg1	α-Ketoglutarate					20:34	Ornithine α-Ketoglutarate	10:34	Ornithine α-Ketoglutarate	10:34	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	7	43	theme	standard	1273:1280	arg1	diet					1282:1285	a standard diet	1271:1285	a standard diet	1271:1285	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	2	44	theme	intestinal	371:380	arg1	dysfunction					395:405	chronic intestinal inflammatory dysfunction	363:405	chronic intestinal inflammatory dysfunction	363:405	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	4	45	theme	mouse	480:484	arg1	model					486:490	a mouse model	478:490	a mouse model of acute colitis	478:507	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	2	46	theme	inflammatory	320:331	arg1	disease					339:345	an inflammatory bowel disease	317:345	an inflammatory bowel disease (IBD)	317:351	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	2	46	theme	inflammatory	320:331	arg1	IBD					348:350	IBD	348:350	IBD	348:350	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	6	47	theme	OKG	1023:1025	arg1	supplementation					1027:1041	1% OKG supplementation	1020:1041	1% OKG supplementation	1020:1041	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	3	48	theme	OKG	451:453	arg1	dosage					441:446	the optimal dosage	429:446	the optimal dosage of OKG in healthy mice	429:469	This study evaluated the optimal dosage of OKG in healthy mice.
37299439	10	49	theme	IL-10	1587:1591	arg1	secretion					1593:1601	serum IL-10 secretion	1581:1601	serum IL-10 secretion	1581:1601	OKG also increased serum IL-10 secretion.
37299439	12	50	theme	biochemical	1922:1932	arg1	indicators					1934:1943	serum biochemical indicators	1916:1943	serum biochemical indicators	1916:1943	Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations.
37299439	2	51	theme	chronic	363:369	arg1	dysfunction					395:405	chronic intestinal inflammatory dysfunction	363:405	chronic intestinal inflammatory dysfunction	363:405	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	1	52	theme	anti-oxidative	221:234	arg1	effects					258:264	anti-oxidative and anti-inflammatory effects	221:264	anti-oxidative and anti-inflammatory effects on humans and animals	221:286	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	53	with	salt	211:214	arg1	effects					258:264	anti-oxidative and anti-inflammatory effects	221:264	anti-oxidative and anti-inflammatory effects on humans and animals	221:286	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	12	54	theme	acid	1955:1958	arg1	concentrations					1960:1973	amino acid concentrations	1949:1973	amino acid concentrations	1949:1973	Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations.
37299439	1	55	theme	anti-inflammatory	240:256	arg1	effects					258:264	anti-oxidative and anti-inflammatory effects	221:264	anti-oxidative and anti-inflammatory effects on humans and animals	221:286	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	11	56	theme	Firmicutes	1644:1653	arg1	abundance					1631:1639	the abundance	1627:1639	the abundance of Firmicutes	1627:1653	Moreover, OKG enhanced the abundance of Firmicutes and decreased that of Bacteriodetes at the phylum level and particularly enhanced the abundance of Alistipes and reduced that of Parabacterioides at the genus level.
37299439	4	57	theme	dextran	531:537	arg1	sulfate					546:552	dextran sodium sulfate	531:552	dextran sodium sulfate (DSS)	531:558	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	4	57	theme	dextran	531:537	arg1	DSS					555:557	DSS	555:557	DSS	555:557	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	6	58	theme	body	1053:1056	arg1	weight					1058:1063	body weight	1053:1063	body weight	1053:1063	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	2	59	theme	Ulcerative	289:298	arg1	UC					309:310	UC	309:310	UC	309:310	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	2	59	theme	Ulcerative	289:298	arg1	colitis					300:306	Ulcerative colitis	289:306	Ulcerative colitis (UC)	289:311	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	6	60	theme	urea	1163:1166	arg1	BUN					1178:1180	BUN	1178:1180	BUN	1178:1180	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	6	60	theme	urea	1163:1166	arg1	nitrogen					1168:1175	urea nitrogen	1163:1175	urea nitrogen (BUN)	1163:1181	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	7	61	theme	main	1342:1345	arg1	design					1220:1225	a 2 × 2 factor design	1205:1225	a 2 × 2 factor design	1205:1225	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	7	61	theme	main	1342:1345	arg1	factors					1347:1353	the main factors	1338:1353	the main factors	1338:1353	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	3	62	theme	healthy	458:464	arg1	mice					466:469	healthy mice	458:469	healthy mice	458:469	This study evaluated the optimal dosage of OKG in healthy mice.
37299439	13	63	theme	microbial	2062:2070	arg1	compositions					2072:2083	microbial compositions	2062:2083	microbial compositions	2062:2083	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	4	64	theme	OKG	590:592	arg1	effect					580:585	the preventive effect	565:585	the preventive effect of OKG on DSS-induced colitis in mice	565:623	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	6	65	theme	%	1021:1021	arg1	supplementation					1027:1041	1% OKG supplementation	1020:1041	1% OKG supplementation	1020:1041	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	5	66	theme	low	789:791	arg1	dose					793:796	a low dose	787:796	a low dose of OKG (LOKG: 0.5%)	787:816	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	4	67	theme	DSS-induced	597:607	arg1	colitis					609:615	DSS-induced colitis	597:615	DSS-induced colitis in mice	597:623	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	4	68	from	effect	580:585	arg1	colitis					609:615	DSS-induced colitis	597:615	DSS-induced colitis in mice	597:623	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	5	69	theme	high	886:889	arg1	dose					891:894	a high dose	884:894	a high dose of OKG (HOKG: 1.5%)	884:914	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	1	70	theme	amino	200:204	arg1	salt					211:214	an amino acid salt	197:214	an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals	197:286	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	70	theme	amino	200:204	arg1	α-ketoglutarate					147:161	Ornithine α-ketoglutarate	137:161	Ornithine α-ketoglutarate (OKG)	137:167	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	7	71	theme	mice	1254:1257	arg1	total					1242:1246	a total	1240:1246	a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not)	1240:1333	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	12	72	theme	hormone	1884:1890	arg1	secretion					1892:1900	hormone secretion	1884:1900	hormone secretion	1884:1900	Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations.
37299439	6	73	theme	growth	1072:1077	arg1	hormone					1079:1085	serum growth hormone	1066:1085	serum growth hormone (GH)	1066:1090	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	6	73	theme	growth	1072:1077	arg1	GH					1088:1089	GH	1088:1089	GH	1088:1089	Our results demonstrated that 1% OKG supplementation increased body weight, serum growth hormone (GH), insulin (INS), alkaline phosphatase (ALP), Tyr, and His and decreased urea nitrogen (BUN), NH3L, and Ile.
37299439	1	74	theme	Ornithine	137:145	arg1	compound					184:191	a nutritional compound	170:191	a nutritional compound	170:191	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	74	theme	Ornithine	137:145	arg1	OKG					164:166	OKG	164:166	OKG	164:166	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	74	theme	Ornithine	137:145	arg1	salt					211:214	an amino acid salt	197:214	an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals	197:286	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	1	74	theme	Ornithine	137:145	arg1	α-ketoglutarate					147:161	Ornithine α-ketoglutarate	137:161	Ornithine α-ketoglutarate (OKG)	137:167	Ornithine α-ketoglutarate (OKG), a nutritional compound, is an amino acid salt with anti-oxidative and anti-inflammatory effects on humans and animals.
37299439	0	75	theme	Inflammatory	71:82	arg1	Cytokines					84:92	Serum Inflammatory Cytokines	65:92	Serum Inflammatory Cytokines	65:92	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	5	76	dep	MOKG	855:858	arg1	%					862:862	1%	861:862	1%	861:862	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	11	77	theme	Alistipes	1754:1762	arg1	abundance					1741:1749	the abundance	1737:1749	the abundance of Alistipes	1737:1762	Moreover, OKG enhanced the abundance of Firmicutes and decreased that of Bacteriodetes at the phylum level and particularly enhanced the abundance of Alistipes and reduced that of Parabacterioides at the genus level.
37299439	9	78	theme	colonic	1518:1524	arg1	damage					1539:1544	colonic histological damage	1518:1544	colonic histological damage induced by DSS	1518:1559	The results revealed that OKG alleviated weight loss and reversed the increases in colonic histological damage induced by DSS.
37299439	7	79	dep	diet	1265:1268	arg1	diet					1282:1285	a standard diet	1271:1285	a standard diet	1271:1285	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	7	79	dep	diet	1265:1268	arg1	diet					1299:1302	a 1% OKG diet	1290:1302	a 1% OKG diet	1290:1302	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	0	80	theme	Sulfate	105:111	arg1	Sodium					113:118	Dextran Sulfate Sodium	97:118	Dextran Sulfate Sodium	97:118	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	13	81	theme	1	1989:1989	arg1	%					1990:1990	%	1990:1990	%	1990:1990	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	4	82	theme	microbiota	697:706	arg1	analysis					646:653	analysis	646:653	analysis of serum inflammatory cytokines and fecal microbiota	646:706	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	13	83	theme	%	1990:1990	arg1	supplementation					1996:2010	1% OKG supplementation	1989:2010	1% OKG supplementation	1989:2010	Furthermore, 1% OKG supplementation prevents DSS-induced colitis in mice via altering microbial compositions and reducing the secretion of inflammatory cytokines in serum.
37299439	7	84	theme	%	1293:1293	arg1	diet					1299:1302	a 1% OKG diet	1290:1302	a 1% OKG diet	1290:1302	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	9	85	from	increases	1505:1513	arg1	damage					1539:1544	colonic histological damage	1518:1544	colonic histological damage induced by DSS	1518:1559	The results revealed that OKG alleviated weight loss and reversed the increases in colonic histological damage induced by DSS.
37299439	11	86	theme	phylum	1698:1703	arg1	level					1705:1709	the phylum level	1694:1709	the phylum level	1694:1709	Moreover, OKG enhanced the abundance of Firmicutes and decreased that of Bacteriodetes at the phylum level and particularly enhanced the abundance of Alistipes and reduced that of Parabacterioides at the genus level.
37299439	7	87	dep	challenge	1309:1317	arg1	%					1321:1321	4%	1320:1321	4% DSS or not	1320:1332	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	4	88	theme	inflammatory	664:675	arg1	cytokines					677:685	serum inflammatory cytokines	658:685	serum inflammatory cytokines	658:685	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	12	89	theme	growth	1861:1866	arg1	performance					1868:1878	growth performance	1861:1878	growth performance	1861:1878	Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations.
37299439	0	90	theme	α-Ketoglutarate	20:34	arg1	Effect					0:5	Effect	0:5	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium	0:118	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	8	91	theme	DSS	1382:1384	arg1	mice					1386:1389	the DSS mice	1378:1389	the DSS mice	1378:1389	During days 14 to 21, the DSS mice were administered 4% DSS to induce colitis.
37299439	8	92	dep	days	1363:1366	arg1	to					1371:1372	to	1371:1372	to	1371:1372	During days 14 to 21, the DSS mice were administered 4% DSS to induce colitis.
37299439	2	93	theme	inflammatory	382:393	arg1	dysfunction					395:405	chronic intestinal inflammatory dysfunction	363:405	chronic intestinal inflammatory dysfunction	363:405	Ulcerative colitis (UC), as an inflammatory bowel disease (IBD), leads to chronic intestinal inflammatory dysfunction.
37299439	0	94	theme	Intestinal	39:48	arg1	Microbiota					50:59	Intestinal Microbiota	39:59	Intestinal Microbiota	39:59	Effect of Ornithine α-Ketoglutarate on Intestinal Microbiota and Serum Inflammatory Cytokines in Dextran Sulfate Sodium Induced Colitis.
37299439	5	95	theme	entire	955:960	arg1	period					982:987	the entire 14-day experimental period	951:987	the entire 14-day experimental period	951:987	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	7	96	theme	×	1209:1209	arg1	design					1220:1225	a 2 × 2 factor design	1205:1225	a 2 × 2 factor design	1205:1225	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	7	96	theme	×	1209:1209	arg1	factors					1347:1353	the main factors	1338:1353	the main factors	1338:1353	Then, a 2 × 2 factor design was used for a total of 40 mice, with diet (a standard diet or a 1% OKG diet) and challenge (4% DSS or not) as the main factors.
37299439	5	97	dep	HOKG	904:907	arg1	%					913:913	1.5%	910:913	1.5%	910:913	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	3	98	theme	optimal	433:439	arg1	dosage					441:446	the optimal dosage	429:446	the optimal dosage of OKG in healthy mice	429:469	This study evaluated the optimal dosage of OKG in healthy mice.
37299439	4	99	theme	acute	495:499	arg1	colitis					501:507	acute colitis	495:507	acute colitis	495:507	Then, a mouse model of acute colitis was established using dextran sodium sulfate (DSS), and the preventive effect of OKG on DSS-induced colitis in mice was explored through analysis of serum inflammatory cytokines and fecal microbiota.
37299439	3	100	from	dosage	441:446	arg1	mice					466:469	healthy mice	458:469	healthy mice	458:469	This study evaluated the optimal dosage of OKG in healthy mice.
37299439	5	101	theme	OKG	850:852	arg1	dose					842:845	a medium dose	833:845	a medium dose of OKG (MOKG: 1%)	833:863	Initially, the mice were randomly divided into a control group, a group given a low dose of OKG (LOKG: 0.5%), a group given a medium dose of OKG (MOKG: 1%), and a group given a high dose of OKG (HOKG: 1.5%); they remained in these groups for the entire 14-day experimental period.
37299439	10	102	theme	serum	1581:1585	arg1	secretion					1593:1601	serum IL-10 secretion	1581:1601	serum IL-10 secretion	1581:1601	OKG also increased serum IL-10 secretion.
37299439	12	103	theme	amino	1949:1953	arg1	concentrations					1960:1973	amino acid concentrations	1949:1973	amino acid concentrations	1949:1973	Our results indicated that OKG promotes growth performance and hormone secretion and regulates serum biochemical indicators and amino acid concentrations.
36562202	12	0	theme	postoperative	2094:2106	arg1	complications					2120:2132	postoperative respiratory complications	2094:2132	postoperative respiratory complications	2094:2132	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	10	1	theme	postoperative	1759:1771	arg1	complications					1785:1797	postoperative respiratory complications	1759:1797	postoperative respiratory complications	1759:1797	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	1	2	theme	respiratory	292:302	arg1	complications					304:316	postoperative respiratory complications	278:316	postoperative respiratory complications	278:316	Reversing neuromuscular blockade with sugammadex can eliminate residual paralysis, which has been associated with postoperative respiratory complications.
36562202	8	3	theme	reversal	1295:1302	arg1	drug					1304:1307	the reversal drug	1291:1307	the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76)	1291:1356	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	8	3	theme	reversal	1295:1302	arg1	OR					1319:1320	adjusted OR	1310:1320	adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76	1310:1355	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	4	4	theme	academic	686:693	arg1	networks					706:713	two academic healthcare networks	682:713	two academic healthcare networks	682:713	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	2	5	theme	equivocal	329:337	arg1	data					339:342	equivocal data	329:342	equivocal data on whether sugammadex reduces these when compared with neostigmine	329:409	There are equivocal data on whether sugammadex reduces these when compared with neostigmine.
36562202	10	6	theme	Equivalence	1593:1603	arg1	testing					1605:1611	Equivalence testing	1593:1611	Equivalence testing	1593:1611	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	7	7	theme	postoperative	1221:1233	arg1	complications					1247:1259	postoperative respiratory complications	1221:1259	postoperative respiratory complications	1221:1259	In total, 5746 (6.9%) of 83,250 included patients experienced postoperative respiratory complications.
36562202	10	8	theme	advanced	1802:1809	arg1	utilisation					1822:1832	advanced healthcare utilisation	1802:1832	advanced healthcare utilisation	1802:1832	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	12	9	theme	blockade	2030:2037	arg1	reversal					2004:2011	reversal	2004:2011	reversal of neuromuscular blockade with sugammadex	2004:2053	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	9	10	theme	reversal	1534:1541	arg1	choice					1524:1529	the choice	1520:1529	the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11)	1520:1590	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	8	11	theme	95	1323:1324	arg1	%					1325:1325	%	1325:1325	%	1325:1325	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	6	12	theme	30-day	1107:1112	arg1	readmission					1123:1133	30-day hospital readmission	1107:1133	30-day hospital readmission	1107:1133	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	10	13	theme	equivalent	1626:1635	arg1	effect					1637:1642	an equivalent effect	1623:1642	an equivalent effect size of sugammadex and neostigmine	1623:1677	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	7	14	theme	83,250	1184:1189	arg1	5746					1169:1172	5746	1169:1172	5746	1169:1172	In total, 5746 (6.9%) of 83,250 included patients experienced postoperative respiratory complications.
36562202	7	14	theme	83,250	1184:1189	arg1	%					1178:1178	6.9%	1175:1178	6.9%	1175:1178	In total, 5746 (6.9%) of 83,250 included patients experienced postoperative respiratory complications.
36562202	7	14	theme	83,250	1184:1189	arg1	83,250					1184:1189	83,250	1184:1189	83,250	1184:1189	In total, 5746 (6.9%) of 83,250 included patients experienced postoperative respiratory complications.
36562202	9	15	theme	adjusted	1544:1551	arg1	reversal					1534:1541	reversal	1534:1541	reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11)	1534:1590	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	9	15	theme	adjusted	1544:1551	arg1	OR					1553:1554	adjusted OR	1544:1554	adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11	1544:1589	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	8	16	theme	CI	1326:1327	arg1	drug					1304:1307	the reversal drug	1291:1307	the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76)	1291:1356	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	8	16	theme	CI	1326:1327	arg1	OR					1319:1320	adjusted OR	1310:1320	adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76	1310:1355	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	10	17	theme	neostigmine	1667:1677	arg1	size					1644:1647	an equivalent effect size	1623:1647	an equivalent effect size of sugammadex and neostigmine	1623:1677	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	0	18	theme	cohort	150:155	arg1	study					157:161	a multicentre retrospective cohort study	122:161	a multicentre retrospective cohort study	122:161	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	6	19	theme	care	1086:1089	arg1	unit					1091:1094	7-day unplanned intensive care unit	1060:1094	: 7-day unplanned intensive care unit admission	1058:1104	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	12	20	theme	healthcare	2162:2171	arg1	utilisation					2173:2183	post-procedural advanced healthcare utilisation	2137:2183	post-procedural advanced healthcare utilisation	2137:2183	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	10	21	theme	sugammadex	1652:1661	arg1	size					1644:1647	an equivalent effect size	1623:1647	an equivalent effect size of sugammadex and neostigmine	1623:1677	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	11	22	theme	reversal	1881:1888	arg1	drug					1890:1893	the reversal drug	1877:1893	the reversal drug	1877:1893	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
36562202	5	23	theme	postoperative	775:787	arg1	complications					801:813	postoperative respiratory complications	775:813	postoperative respiratory complications	775:813	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	5	23	theme	postoperative	775:787	arg1	outcome					763:769	The primary outcome	751:769	The primary outcome	751:769	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	9	24	theme	%	1559:1559	arg1	CI					1560:1561	95%CI	1557:1561	adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11	1544:1589	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	6	25	theme	unplanned	1066:1074	arg1	unit					1091:1094	7-day unplanned intensive care unit	1060:1094	: 7-day unplanned intensive care unit admission	1058:1104	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	0	26	theme	respiratory	59:69	arg1	complications					71:83	postoperative respiratory complications	45:83	postoperative respiratory complications	45:83	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	1	27	theme	Reversing	164:172	arg1	blockade					188:195	Reversing neuromuscular blockade	164:195	Reversing neuromuscular blockade with sugammadex	164:211	Reversing neuromuscular blockade with sugammadex can eliminate residual paralysis, which has been associated with postoperative respiratory complications.
36562202	12	28	theme	post-procedural	2137:2151	arg1	utilisation					2173:2183	post-procedural advanced healthcare utilisation	2137:2183	post-procedural advanced healthcare utilisation	2137:2183	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	12	29	with	blockade	2030:2037	arg1	sugammadex					2044:2053	sugammadex	2044:2053	sugammadex	2044:2053	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	1	30	with	blockade	188:195	arg1	sugammadex					202:211	sugammadex	202:211	sugammadex	202:211	Reversing neuromuscular blockade with sugammadex can eliminate residual paralysis, which has been associated with postoperative respiratory complications.
36562202	0	31	theme	healthcare	98:107	arg1	utilisation					109:119	advanced healthcare utilisation	89:119	advanced healthcare utilisation	89:119	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	9	32	theme	skilled	1398:1404	arg1	facilities					1414:1423	skilled nursing facilities	1398:1423	skilled nursing facilities	1398:1423	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	3	33	theme	respiratory	494:504	arg1	complications					506:518	postoperative respiratory complications	480:518	postoperative respiratory complications	480:518	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	5	34	theme	%	865:865	arg1	saturation					850:859	post-extubation oxygen saturation	827:859	post-extubation oxygen saturation < 90%	827:865	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	5	35	theme	non-invasive	898:909	arg1	ventilation					911:921	non-invasive ventilation	898:921	non-invasive ventilation	898:921	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	3	36	theme	healthcare	533:542	arg1	utilisation					544:554	advanced healthcare utilisation	524:554	advanced healthcare utilisation	524:554	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	6	37	theme	healthcare	1005:1014	arg1	utilisation					1016:1026	advanced healthcare utilisation	996:1026	advanced healthcare utilisation	996:1026	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	6	37	theme	healthcare	1005:1014	arg1	outcome					1041:1047	a composite outcome	1029:1047	a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge	1029:1156	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	3	38	theme	reversal	461:468	arg1	drug					470:473	reversal drug	461:473	reversal drug	461:473	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	3	39	theme	choice	451:456	arg1	association					432:442	the association	428:442	the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation	428:554	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	5	40	theme	respiratory	868:878	arg1	failure					880:886	respiratory failure	868:886	respiratory failure requiring non-invasive ventilation	868:921	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	6	41	theme	secondary	974:982	arg1	outcome					984:990	Our main secondary outcome	965:990	Our main secondary outcome	965:990	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	4	42	theme	general	619:625	arg1	anaesthesia					627:637	general anaesthesia	619:637	general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021	619:748	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	8	43	dep	OR	1319:1320	arg1	p = 0.76					1348:1355	p = 0.76	1348:1355	p = 0.76	1348:1355	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	9	44	dep	OR	1553:1554	arg1	p = 0.11					1582:1589	p = 0.11	1582:1589	p = 0.11	1582:1589	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	11	45	theme	adverse	1905:1911	arg1	events					1928:1933	major adverse cardiovascular events	1899:1933	major adverse cardiovascular events	1899:1933	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
36562202	9	46	theme	healthcare	1466:1475	arg1	utilisation					1477:1487	advanced healthcare utilisation	1457:1487	advanced healthcare utilisation	1457:1487	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	7	47	theme	respiratory	1235:1245	arg1	complications					1247:1259	postoperative respiratory complications	1221:1259	postoperative respiratory complications	1221:1259	In total, 5746 (6.9%) of 83,250 included patients experienced postoperative respiratory complications.
36562202	4	48	theme	healthcare	695:704	arg1	networks					706:713	two academic healthcare networks	682:713	two academic healthcare networks	682:713	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	6	49	theme	7-day	1060:1064	arg1	unit					1091:1094	7-day unplanned intensive care unit	1060:1094	: 7-day unplanned intensive care unit admission	1058:1104	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	4	50	from	networks	706:713	arg1	sugammadex					644:653	sugammadex	644:653	sugammadex	644:653	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	4	50	from	networks	706:713	arg1	reversal					670:677	neostigmine reversal	658:677	neostigmine reversal	658:677	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	10	51	theme	respiratory	1773:1783	arg1	complications					1785:1797	postoperative respiratory complications	1759:1797	postoperative respiratory complications	1759:1797	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	1	52	theme	postoperative	278:290	arg1	complications					304:316	postoperative respiratory complications	278:316	postoperative respiratory complications	278:316	Reversing neuromuscular blockade with sugammadex can eliminate residual paralysis, which has been associated with postoperative respiratory complications.
36562202	12	53	from	reduction	2081:2089	arg1	utilisation					2173:2183	post-procedural advanced healthcare utilisation	2137:2183	post-procedural advanced healthcare utilisation	2137:2183	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	12	53	from	reduction	2081:2089	arg1	complications					2120:2132	postoperative respiratory complications	2094:2132	postoperative respiratory complications	2094:2132	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	5	54	theme	primary	755:761	arg1	complications					801:813	postoperative respiratory complications	775:813	postoperative respiratory complications	775:813	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	5	54	theme	primary	755:761	arg1	outcome					763:769	The primary outcome	751:769	The primary outcome	751:769	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	6	55	theme	composite	1031:1039	arg1	utilisation					1016:1026	advanced healthcare utilisation	996:1026	advanced healthcare utilisation	996:1026	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	6	55	theme	composite	1031:1039	arg1	outcome					1041:1047	a composite outcome	1029:1047	a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge	1029:1156	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	10	56	theme	healthcare	1811:1820	arg1	utilisation					1822:1832	advanced healthcare utilisation	1802:1832	advanced healthcare utilisation	1802:1832	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	12	57	theme	neuromuscular	2016:2028	arg1	blockade					2030:2037	neuromuscular blockade	2016:2037	neuromuscular blockade with sugammadex	2016:2053	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	4	58	with	anaesthesia	627:637	arg1	sugammadex					644:653	sugammadex	644:653	sugammadex	644:653	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	4	58	with	anaesthesia	627:637	arg1	reversal					670:677	neostigmine reversal	658:677	neostigmine reversal	658:677	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	8	59	theme	adjusted	1310:1317	arg1	drug					1304:1307	the reversal drug	1291:1307	the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76)	1291:1356	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	8	59	theme	adjusted	1310:1317	arg1	OR					1319:1320	adjusted OR	1310:1320	adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76	1310:1355	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	6	60	theme	hospital	1114:1121	arg1	readmission					1123:1133	30-day hospital readmission	1107:1133	30-day hospital readmission	1107:1133	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	3	61	with	association	432:442	arg1	complications					506:518	postoperative respiratory complications	480:518	postoperative respiratory complications	480:518	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	3	61	with	association	432:442	arg1	utilisation					544:554	advanced healthcare utilisation	524:554	advanced healthcare utilisation	524:554	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	10	62	theme	effect	1637:1642	arg1	size					1644:1647	an equivalent effect size	1623:1647	an equivalent effect size of sugammadex and neostigmine	1623:1677	Equivalence testing supported an equivalent effect size of sugammadex and neostigmine on both outcomes, and neostigmine was non-inferior to sugammadex with regard to postoperative respiratory complications or advanced healthcare utilisation.
36562202	0	63	theme	retrospective	136:148	arg1	study					157:161	a multicentre retrospective cohort study	122:161	a multicentre retrospective cohort study	122:161	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	1	64	theme	residual	227:234	arg1	paralysis					236:244	residual paralysis	227:244	residual paralysis	227:244	Reversing neuromuscular blockade with sugammadex can eliminate residual paralysis, which has been associated with postoperative respiratory complications.
36562202	8	65	theme	%	1325:1325	arg1	CI					1326:1327	95%CI	1323:1327	adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76	1310:1355	This was not associated with the reversal drug (adjusted OR (95%CI) 1.01 (0.94-1.08); p = 0.76).
36562202	6	66	theme	unit	1091:1094	arg1	admission					1096:1104	: 7-day unplanned intensive care unit admission	1058:1104	: 7-day unplanned intensive care unit admission	1058:1104	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	11	67	theme	major	1899:1903	arg1	events					1928:1933	major adverse cardiovascular events	1899:1933	major adverse cardiovascular events	1899:1933	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
36562202	9	68	theme	95	1557:1558	arg1	%					1559:1559	%	1559:1559	%	1559:1559	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	6	69	theme	intensive	1076:1084	arg1	unit					1091:1094	7-day unplanned intensive care unit	1060:1094	: 7-day unplanned intensive care unit admission	1058:1104	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	12	70	theme	advanced	2153:2160	arg1	utilisation					2173:2183	post-procedural advanced healthcare utilisation	2137:2183	post-procedural advanced healthcare utilisation	2137:2183	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	5	71	theme	respiratory	789:799	arg1	complications					801:813	postoperative respiratory complications	775:813	postoperative respiratory complications	775:813	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	5	71	theme	respiratory	789:799	arg1	outcome					763:769	The primary outcome	751:769	The primary outcome	751:769	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	9	72	theme	CI	1560:1561	arg1	reversal					1534:1541	reversal	1534:1541	reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11)	1534:1590	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	9	72	theme	CI	1560:1561	arg1	OR					1553:1554	adjusted OR	1544:1554	adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11	1544:1589	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	11	73	dep	was	1850:1852	arg1	0.94-1.21					1954:1962	0.94-1.21	1954:1962	0.94-1.21	1954:1962	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
36562202	11	73	dep	was	1850:1852	arg1	1.07					1948:1951	1.07	1948:1951	1.07	1948:1951	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
36562202	11	73	dep	was	1850:1852	arg1	adjusted					1936:1943	adjusted	1936:1943	adjusted	1936:1943	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
36562202	0	74	dep	study	157:161	arg1	effects					4:10	The effects	0:10	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation	0:119	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	12	75	theme	respiratory	2108:2118	arg1	complications					2120:2132	postoperative respiratory complications	2094:2132	postoperative respiratory complications	2094:2132	Compared with neostigmine, reversal of neuromuscular blockade with sugammadex was not associated with a reduction in postoperative respiratory complications or post-procedural advanced healthcare utilisation.
36562202	0	76	theme	advanced	89:96	arg1	utilisation					109:119	advanced healthcare utilisation	89:119	advanced healthcare utilisation	89:119	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	1	77	theme	neuromuscular	174:186	arg1	blockade					188:195	Reversing neuromuscular blockade	164:195	Reversing neuromuscular blockade with sugammadex	164:211	Reversing neuromuscular blockade with sugammadex can eliminate residual paralysis, which has been associated with postoperative respiratory complications.
36562202	6	78	theme	non-home	1139:1146	arg1	discharge					1148:1156	non-home discharge	1139:1156	non-home discharge	1139:1156	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	0	79	theme	multicentre	124:134	arg1	study					157:161	a multicentre retrospective cohort study	122:161	a multicentre retrospective cohort study	122:161	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	0	80	from	effects	4:10	arg1	utilisation					109:119	advanced healthcare utilisation	89:119	advanced healthcare utilisation	89:119	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	0	80	from	effects	4:10	arg1	complications					71:83	postoperative respiratory complications	45:83	postoperative respiratory complications	45:83	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	5	81	theme	oxygen	843:848	arg1	saturation					850:859	post-extubation oxygen saturation	827:859	post-extubation oxygen saturation < 90%	827:865	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	5	82	theme	< 90	861:864	arg1	%					865:865	%	865:865	%	865:865	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	9	83	theme	nursing	1406:1412	arg1	facilities					1414:1423	skilled nursing facilities	1398:1423	skilled nursing facilities	1398:1423	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	4	84	theme	adult	569:573	arg1	patients					575:582	adult patients	569:582	adult patients who underwent surgery	569:604	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	5	85	theme	tracheal	927:934	arg1	re-intubation					936:948	tracheal re-intubation	927:948	tracheal re-intubation	927:948	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	11	86	dep	adjusted	1936:1943	arg1	p = 0.32					1966:1973	p = 0.32	1966:1973	p = 0.32	1966:1973	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
36562202	3	87	theme	postoperative	480:492	arg1	complications					506:518	postoperative respiratory complications	480:518	postoperative respiratory complications	480:518	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	0	88	theme	sugammadex	15:24	arg1	effects					4:10	The effects	0:10	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation	0:119	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	4	89	theme	neostigmine	658:668	arg1	reversal					670:677	neostigmine reversal	658:677	neostigmine reversal	658:677	We included adult patients who underwent surgery and received general anaesthesia with sugammadex or neostigmine reversal at two academic healthcare networks between January 2016 and June 2021.
36562202	0	90	theme	neostigmine	30:40	arg1	effects					4:10	The effects	0:10	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation	0:119	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	6	91	theme	advanced	996:1003	arg1	utilisation					1016:1026	advanced healthcare utilisation	996:1026	advanced healthcare utilisation	996:1026	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	6	91	theme	advanced	996:1003	arg1	outcome					1041:1047	a composite outcome	1029:1047	a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge	1029:1156	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	3	92	theme	drug	470:473	arg1	choice					451:456	the choice	447:456	the choice of reversal drug	447:473	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	3	93	theme	advanced	524:531	arg1	utilisation					544:554	advanced healthcare utilisation	524:554	advanced healthcare utilisation	524:554	We investigated the association of the choice of reversal drug with postoperative respiratory complications and advanced healthcare utilisation.
36562202	0	94	theme	postoperative	45:57	arg1	complications					71:83	postoperative respiratory complications	45:83	postoperative respiratory complications	45:83	The effects of sugammadex vs. neostigmine on postoperative respiratory complications and advanced healthcare utilisation: a multicentre retrospective cohort study.
36562202	5	95	theme	post-extubation	827:841	arg1	saturation					850:859	post-extubation oxygen saturation	827:859	post-extubation oxygen saturation < 90%	827:865	The primary outcome was postoperative respiratory complications, defined as post-extubation oxygen saturation < 90%, respiratory failure requiring non-invasive ventilation, or tracheal re-intubation within 7 days.
36562202	9	96	theme	advanced	1457:1464	arg1	utilisation					1477:1487	advanced healthcare utilisation	1457:1487	advanced healthcare utilisation	1457:1487	After excluding patients admitted from skilled nursing facilities, 8372 (10.5%) patients required advanced healthcare utilisation, which was not associated with the choice of reversal (adjusted OR (95%CI) 0.95 (0.89-1.01); p = 0.11).
36562202	6	97	theme	main	969:972	arg1	outcome					984:990	Our main secondary outcome	965:990	Our main secondary outcome	965:990	Our main secondary outcome was advanced healthcare utilisation, a composite outcome including: 7-day unplanned intensive care unit admission; 30-day hospital readmission; or non-home discharge.
36562202	11	98	theme	cardiovascular	1913:1926	arg1	events					1928:1933	major adverse cardiovascular events	1899:1933	major adverse cardiovascular events	1899:1933	Finally, there was no association between the reversal drug and major adverse cardiovascular events (adjusted OR 1.07 (0.94-1.21); p = 0.32).
37099000	2	0	theme	potential	362:370	arg1	mechanisms					372:381	the potential mechanisms	358:381	the potential mechanisms of B. pseudolongum CCFM1253	358:409	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	4	1	theme	antioxidative	891:903	arg1	enzymes					905:911	elevated antioxidative enzymes	882:911	elevated antioxidative enzymes activities	882:922	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	8	2	theme	oxidative	1904:1912	arg1	stress					1914:1919	oxidative stress	1904:1919	oxidative stress	1904:1919	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	3	3	theme	CCFM1253	571:578	arg1	preintervention					580:594	B. pseudolongum CCFM1253 preintervention	555:594	RESULTS B. pseudolongum CCFM1253 preintervention	547:594	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	6	4	theme	metabolites-related	1577:1595	arg1	metabolism					1608:1617	liver metabolites-related riboflavin metabolism	1571:1617	liver metabolites-related riboflavin metabolism	1571:1617	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	3	5	dep	RESULTS	547:553	arg1	preintervention					580:594	B. pseudolongum CCFM1253 preintervention	555:594	RESULTS B. pseudolongum CCFM1253 preintervention	547:594	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	7	6	theme	HepG2	1803:1807	arg1	cells					1809:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	7	7	from	contents	1744:1751	arg1	cells					1809:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	6	8	theme	liver	1449:1453	arg1	metabolomics					1455:1466	Untargeted liver metabolomics	1438:1466	Untargeted liver metabolomics	1438:1466	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	4	9	dep	-α	843:844	arg1	capacity					943:950	[total antioxidant capacity	924:950	[total antioxidant capacity	924:950	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	7	10	theme	hydrogen	1777:1784	arg1	cells					1809:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	5	11	theme	oxidative	1420:1428	arg1	stress					1430:1435	oxidative stress	1420:1435	oxidative stress	1420:1435	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	7	12	theme	CAT	1770:1772	arg1	contents					1744:1751	the contents	1740:1751	the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells	1740:1813	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	8	13	theme	intestinal	1939:1948	arg1	composition					1961:1971	the intestinal microbiota composition	1935:1971	the intestinal microbiota composition	1935:1971	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	5	14	from	UCG-010	1318:1324	arg1	mice					1333:1336	ALI mice	1329:1336	ALI mice	1329:1336	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	8	15	dep	CONCLUSION	1816:1825	arg1	pseudolongum					1830:1841	CONCLUSION B. pseudolongum	1816:1841	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.	1816:2055	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	8	15	dep	CONCLUSION	1816:1825	arg1	regulate					1926:1933	regulate	1926:1933	regulate the intestinal microbiota composition and liver metabolism	1926:1992	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	8	15	dep	CONCLUSION	1816:1825	arg1	elevate					1999:2005	elevate	1999:2005	elevate the liver riboflavin content in LPS-treated mice	1999:2054	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	8	15	dep	CONCLUSION	1816:1825	arg1	alleviate					1868:1876	alleviate	1868:1876	alleviate inflammatory response and oxidative stress	1868:1919	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	0	16	theme	liver	158:162	arg1	injury					164:169	the acute liver injury	148:169	the acute liver injury in mice	148:177	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	6	17	theme	Untargeted	1438:1447	arg1	metabolomics					1455:1466	Untargeted liver metabolomics	1438:1466	Untargeted liver metabolomics	1438:1466	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	4	18	theme	CCFM1253	741:748	arg1	preintervention					750:764	B. pseudolongum CCFM1253 preintervention	725:764	B. pseudolongum CCFM1253 preintervention	725:764	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	8	19	theme	liver	1977:1981	arg1	metabolism					1983:1992	liver metabolism	1977:1992	liver metabolism	1977:1992	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	7	20	theme	SOD	1761:1763	arg1	contents					1744:1751	the contents	1740:1751	the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells	1740:1813	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	4	21	theme	antioxidant	931:941	arg1	capacity					943:950	[total antioxidant capacity	924:950	[total antioxidant capacity	924:950	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	5	22	theme	Bifidobacterium	1188:1202	arg1	proportion					1160:1169	the proportion	1156:1169	the proportion of Alistipes and Bifidobacterium	1156:1202	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	23	theme	Muribaculum	1273:1283	arg1	UCG-010					1318:1324	Muribaculum, Parasutterella, Ruminococcaceae UCG-010	1273:1324	UCG-010	1318:1324	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	23	theme	Muribaculum	1273:1283	arg1	bacterium					1262:1270	uncultured Bacteroidales bacterium	1237:1270	uncultured Bacteroidales bacterium	1237:1270	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	23	theme	Muribaculum	1273:1283	arg1	Ruminococcaceae					1302:1316	Ruminococcaceae	1302:1316	Ruminococcaceae	1302:1316	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	23	theme	Muribaculum	1273:1283	arg1	Parasutterella					1286:1299	Parasutterella	1286:1299	Parasutterella	1286:1299	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	6	24	theme	phenylalanine	1620:1632	arg1	metabolism					1634:1643	phenylalanine metabolism	1620:1643	phenylalanine metabolism	1620:1643	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	7	25	theme	MDA	1756:1758	arg1	contents					1744:1751	the contents	1740:1751	the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells	1740:1813	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	8	26	from	content	2028:2034	arg1	mice					2051:2054	LPS-treated mice	2039:2054	LPS-treated mice	2039:2054	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	0	27	theme	potential	67:75	arg1	mechanisms					77:86	the potential mechanisms	63:86	the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253	63:137	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	3	28	theme	alanine	648:654	arg1	ALT					670:672	ALT	670:672	ALT	670:672	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	3	28	theme	alanine	648:654	arg1	transaminase					656:667	serum alanine transaminase	642:667	serum alanine transaminase (ALT)	642:673	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	2	29	theme	metagenomic	494:504	arg1	analysis					506:513	metagenomic analysis	494:513	metagenomic analysis	494:513	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	4	30	theme	B.	725:726	arg1	preintervention					750:764	B. pseudolongum CCFM1253 preintervention	725:764	B. pseudolongum CCFM1253 preintervention	725:764	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	5	31	theme	uncultured	1237:1246	arg1	UCG-010					1318:1324	Muribaculum, Parasutterella, Ruminococcaceae UCG-010	1273:1324	UCG-010	1318:1324	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	31	theme	uncultured	1237:1246	arg1	bacterium					1262:1270	uncultured Bacteroidales bacterium	1237:1270	uncultured Bacteroidales bacterium	1237:1270	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	8	32	theme	liver	2011:2015	arg1	content					2028:2034	the liver riboflavin content	2007:2034	the liver riboflavin content in LPS-treated mice	2007:2054	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	8	33	theme	B.	1827:1828	arg1	CONCLUSION					1816:1825	CONCLUSION B. pseudolongum	1816:1841	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.	1816:2055	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	1	34	theme	BACKGROUND	180:189	arg1	pseudolongum					207:218	BACKGROUND Bifidobacterium pseudolongum	180:218	BACKGROUND Bifidobacterium pseudolongum	180:218	BACKGROUND Bifidobacterium pseudolongum is widely exists in mammal gut and its abundance is associated with human and animal health.
37099000	4	35	theme	superoxide	961:970	arg1	dismutase					972:980	superoxide dismutase	961:980	superoxide dismutase (SOD)	961:986	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	35	theme	superoxide	961:970	arg1	responses					805:813	the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC)	788:958	the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC)	788:958	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	35	theme	superoxide	961:970	arg1	SOD					983:985	SOD	983:985	SOD	983:985	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	36	theme	necrosis	822:829	arg1	-α					843:844	[tumor necrosis factor (TNF)-α	815:844	[tumor necrosis factor (TNF)-α	815:844	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	36	theme	necrosis	822:829	arg1	T-AOC					953:957	T-AOC	953:957	T-AOC	953:957	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	5	37	theme	bacterium	1262:1270	arg1	proportion					1223:1232	the proportion	1219:1232	the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress	1219:1435	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	0	38	theme	Bifidobacterium	101:115	arg1	CCFM1253					130:137	probiotic Bifidobacterium pseudolongum CCFM1253	91:137	probiotic Bifidobacterium pseudolongum CCFM1253	91:137	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	2	39	theme	metabolomic	525:535	arg1	profiles					537:544	liver metabolomic profiles	519:544	liver metabolomic profiles	519:544	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	9	40	theme	B.	2068:2069	arg1	CCFM1253					2084:2091	B. pseudolongum CCFM1253	2068:2091	B. pseudolongum CCFM1253	2068:2091	Therefore, B. pseudolongum CCFM1253 could serves as a potential probiotic to ameliorate the host health.
37099000	9	40	theme	B.	2068:2069	arg1	probiotic					2121:2129	probiotic	2121:2129	probiotic	2121:2129	Therefore, B. pseudolongum CCFM1253 could serves as a potential probiotic to ameliorate the host health.
37099000	2	41	dep	B.	386:387	arg1	pseudolongum					389:400	pseudolongum	389:400	pseudolongum	389:400	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	0	42	theme	CCFM1253	130:137	arg1	mechanisms					77:86	the potential mechanisms	63:86	the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253	63:137	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	6	43	theme	TCA	1670:1672	arg1	cycle					1663:1667	citrate cycle	1655:1667	citrate cycle (TCA cycle)	1655:1679	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	6	43	theme	TCA	1670:1672	arg1	cycle					1674:1678	TCA cycle	1670:1678	TCA cycle	1670:1678	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	0	44	dep	Bifidobacterium	101:115	arg1	pseudolongum					117:128	pseudolongum	117:128	pseudolongum	117:128	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	2	45	theme	liver	472:476	arg1	ALI					486:488	ALI	486:488	ALI	486:488	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	2	45	theme	liver	472:476	arg1	injury					478:483	acute liver injury	466:483	lipopolysaccharide (LPS)-induced acute liver injury (ALI)	433:489	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	2	46	theme	CCFM1253	402:409	arg1	mechanisms					372:381	the potential mechanisms	358:381	the potential mechanisms of B. pseudolongum CCFM1253	358:409	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	0	47	theme	Intestinal	0:9	arg1	microbiomics					11:22	Intestinal microbiomics	0:22	Intestinal microbiomics	0:22	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	1	48	theme	animal	298:303	arg1	health					305:310	human and animal health	288:310	human and animal health	288:310	BACKGROUND Bifidobacterium pseudolongum is widely exists in mammal gut and its abundance is associated with human and animal health.
37099000	8	49	theme	inflammatory	1878:1889	arg1	response					1891:1898	inflammatory response	1878:1898	inflammatory response	1878:1898	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	2	50	theme	-induced	457:464	arg1	ALI					486:488	ALI	486:488	ALI	486:488	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	2	50	theme	-induced	457:464	arg1	injury					478:483	acute liver injury	466:483	lipopolysaccharide (LPS)-induced acute liver injury (ALI)	433:489	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	3	51	theme	aspartate	679:687	arg1	transferase					695:705	aspartate amino transferase	679:705	aspartate amino transferase (AST)	679:711	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	3	51	theme	aspartate	679:687	arg1	AST					708:710	AST	708:710	AST	708:710	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	0	52	theme	metabolomics	36:47	arg1	insights					49:56	hepatic metabolomics insights	28:56	hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253	28:137	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	5	53	theme	B.	1112:1113	arg1	treatment					1137:1145	B. pseudolongum CCFM1253 treatment	1112:1145	B. pseudolongum CCFM1253 treatment	1112:1145	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	54	theme	CCFM1253	1128:1135	arg1	treatment					1137:1145	B. pseudolongum CCFM1253 treatment	1112:1145	B. pseudolongum CCFM1253 treatment	1112:1145	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	55	theme	inflammation	1393:1404	arg1	inhibition					1379:1388	the inhibition	1375:1388	the inhibition of inflammation responses and oxidative stress	1375:1435	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	6	56	theme	B.	1516:1517	arg1	CCFM1253					1532:1539	B. pseudolongum CCFM1253	1516:1539	B. pseudolongum CCFM1253	1516:1539	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	3	57	theme	B.	555:556	arg1	preintervention					580:594	B. pseudolongum CCFM1253 preintervention	555:594	RESULTS B. pseudolongum CCFM1253 preintervention	547:594	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	3	58	theme	transferase	695:705	arg1	activities					713:722	serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities	642:722	serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities	642:722	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	2	59	theme	B.	386:387	arg1	CCFM1253					402:409	B. pseudolongum CCFM1253	386:409	B. pseudolongum CCFM1253	386:409	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	8	60	theme	riboflavin	2017:2026	arg1	content					2028:2034	the liver riboflavin content	2007:2034	the liver riboflavin content in LPS-treated mice	2007:2054	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	5	61	theme	Alistipes	1174:1182	arg1	proportion					1160:1169	the proportion	1156:1169	the proportion of Alistipes and Bifidobacterium	1156:1202	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	3	62	theme	pseudolongum	558:569	arg1	preintervention					580:594	B. pseudolongum CCFM1253 preintervention	555:594	RESULTS B. pseudolongum CCFM1253 preintervention	547:594	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	1	63	theme	human	288:292	arg1	health					305:310	human and animal health	288:310	human and animal health	288:310	BACKGROUND Bifidobacterium pseudolongum is widely exists in mammal gut and its abundance is associated with human and animal health.
37099000	4	64	theme	elevated	882:889	arg1	enzymes					905:911	elevated antioxidative enzymes	882:911	elevated antioxidative enzymes activities	882:922	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	7	65	theme	peroxide-treated	1786:1801	arg1	cells					1809:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	hydrogen peroxide-treated HepG2 cells	1777:1813	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	6	66	theme	liver	1571:1575	arg1	metabolism					1608:1617	liver metabolites-related riboflavin metabolism	1571:1617	liver metabolites-related riboflavin metabolism	1571:1617	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	1	67	theme	Bifidobacterium	191:205	arg1	pseudolongum					207:218	BACKGROUND Bifidobacterium pseudolongum	180:218	BACKGROUND Bifidobacterium pseudolongum	180:218	BACKGROUND Bifidobacterium pseudolongum is widely exists in mammal gut and its abundance is associated with human and animal health.
37099000	2	68	theme	study	333:337	arg1	purpose					317:323	The purpose	313:323	The purpose of this study	313:337	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	0	69	theme	acute	152:156	arg1	injury					164:169	the acute liver injury	148:169	the acute liver injury in mice	148:177	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	5	70	theme	stress	1430:1435	arg1	inhibition					1379:1388	the inhibition	1375:1388	the inhibition of inflammation responses and oxidative stress	1375:1435	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	4	71	theme	Nrf2	1083:1086	arg1	pathways					1088:1095	Nrf2 pathways	1083:1095	Nrf2 pathways	1083:1095	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	3	72	theme	LPS	635:637	arg1	influence					622:630	the influence	618:630	the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities	618:722	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	6	73	theme	CCFM1253	1532:1539	arg1	efficacy					1504:1511	the hepatoprotective efficacy	1483:1511	the hepatoprotective efficacy of B. pseudolongum CCFM1253	1483:1539	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	1	74	theme	mammal	240:245	arg1	gut					247:249	mammal gut	240:249	mammal gut	240:249	BACKGROUND Bifidobacterium pseudolongum is widely exists in mammal gut and its abundance is associated with human and animal health.
37099000	4	75	dep	responses	805:813	arg1	-α					843:844	[tumor necrosis factor (TNF)-α	815:844	[tumor necrosis factor (TNF)-α	815:844	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	75	dep	responses	805:813	arg1	T-AOC					953:957	T-AOC	953:957	T-AOC	953:957	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	75	dep	responses	805:813	arg1	IL-6					872:875	IL-6	872:875	IL-6	872:875	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	75	dep	responses	805:813	arg1	activities					913:922	elevated antioxidative enzymes activities	882:922	elevated antioxidative enzymes activities	882:922	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	75	dep	responses	805:813	arg1	interleukin					847:857	interleukin (IL)-1β	847:865	interleukin (IL)-1β	847:865	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	75	dep	responses	805:813	arg1	IL					860:861	IL	860:861	IL	860:861	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	76	theme	enzymes	905:911	arg1	activities					913:922	elevated antioxidative enzymes activities	882:922	elevated antioxidative enzymes activities	882:922	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	3	77	theme	transaminase	656:667	arg1	activities					713:722	serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities	642:722	serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities	642:722	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	4	78	theme	[total	924:929	arg1	capacity					943:950	[total antioxidant capacity	924:950	[total antioxidant capacity	924:950	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	3	79	theme	serum	642:646	arg1	ALT					670:672	ALT	670:672	ALT	670:672	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	3	79	theme	serum	642:646	arg1	transaminase					656:667	serum alanine transaminase	642:667	serum alanine transaminase (ALT)	642:673	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	0	80	theme	probiotic	91:99	arg1	CCFM1253					130:137	probiotic Bifidobacterium pseudolongum CCFM1253	91:137	probiotic Bifidobacterium pseudolongum CCFM1253	91:137	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	6	81	theme	riboflavin	1597:1606	arg1	metabolism					1608:1617	liver metabolites-related riboflavin metabolism	1571:1617	liver metabolites-related riboflavin metabolism	1571:1617	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	5	82	theme	Bacteroidales	1248:1260	arg1	UCG-010					1318:1324	Muribaculum, Parasutterella, Ruminococcaceae UCG-010	1273:1324	UCG-010	1318:1324	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	82	theme	Bacteroidales	1248:1260	arg1	bacterium					1262:1270	uncultured Bacteroidales bacterium	1237:1270	uncultured Bacteroidales bacterium	1237:1270	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	4	83	dep	B.	725:726	arg1	pseudolongum					728:739	pseudolongum	728:739	pseudolongum	728:739	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	84	theme	[tumor	815:820	arg1	-α					843:844	[tumor necrosis factor (TNF)-α	815:844	[tumor necrosis factor (TNF)-α	815:844	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	84	theme	[tumor	815:820	arg1	T-AOC					953:957	T-AOC	953:957	T-AOC	953:957	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	5	85	theme	ALI	1329:1331	arg1	mice					1333:1336	ALI mice	1329:1336	ALI mice	1329:1336	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	5	86	dep	inflammation	1393:1404	arg1	responses					1406:1414	responses	1406:1414	responses	1406:1414	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	2	87	theme	liver	519:523	arg1	profiles					537:544	liver metabolomic profiles	519:544	liver metabolomic profiles	519:544	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	9	88	theme	pseudolongum	2071:2082	arg1	CCFM1253					2084:2091	B. pseudolongum CCFM1253	2068:2091	B. pseudolongum CCFM1253	2068:2091	Therefore, B. pseudolongum CCFM1253 could serves as a potential probiotic to ameliorate the host health.
37099000	9	88	theme	pseudolongum	2071:2082	arg1	probiotic					2121:2129	probiotic	2121:2129	probiotic	2121:2129	Therefore, B. pseudolongum CCFM1253 could serves as a potential probiotic to ameliorate the host health.
37099000	4	89	theme	factor	831:836	arg1	-α					843:844	[tumor necrosis factor (TNF)-α	815:844	[tumor necrosis factor (TNF)-α	815:844	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	89	theme	factor	831:836	arg1	T-AOC					953:957	T-AOC	953:957	T-AOC	953:957	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	7	90	theme	riboflavin	1706:1715	arg1	exposure					1717:1724	riboflavin exposure	1706:1724	riboflavin exposure	1706:1724	Furthermore, riboflavin exposure could control the contents of MDA, SOD, and CAT in hydrogen peroxide-treated HepG2 cells.
37099000	2	91	theme	acute	466:470	arg1	ALI					486:488	ALI	486:488	ALI	486:488	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	2	91	theme	acute	466:470	arg1	injury					478:483	acute liver injury	466:483	lipopolysaccharide (LPS)-induced acute liver injury (ALI)	433:489	The purpose of this study was to investigate the potential mechanisms of B. pseudolongum CCFM1253 on protecting against lipopolysaccharide (LPS)-induced acute liver injury (ALI) by metagenomic analysis and liver metabolomic profiles.
37099000	9	92	theme	host	2149:2152	arg1	health					2154:2159	the host health	2145:2159	the host health	2145:2159	Therefore, B. pseudolongum CCFM1253 could serves as a potential probiotic to ameliorate the host health.
37099000	3	93	from	influence	622:630	arg1	activities					713:722	serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities	642:722	serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities	642:722	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	0	94	theme	hepatic	28:34	arg1	insights					49:56	hepatic metabolomics insights	28:56	hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253	28:137	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	6	95	theme	citrate	1655:1661	arg1	cycle					1663:1667	citrate cycle	1655:1667	citrate cycle (TCA cycle)	1655:1679	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	6	95	theme	citrate	1655:1661	arg1	cycle					1674:1678	TCA cycle	1670:1678	TCA cycle	1670:1678	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	4	96	theme	inflammation	792:803	arg1	dismutase					972:980	superoxide dismutase	961:980	superoxide dismutase (SOD)	961:986	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	96	theme	inflammation	792:803	arg1	responses					805:813	the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC)	788:958	the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC)	788:958	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	96	theme	inflammation	792:803	arg1	peroxidase					1021:1030	glutathione peroxidase	1009:1030	glutathione peroxidase (GSH-Px)]	1009:1040	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	96	theme	inflammation	792:803	arg1	catalase					989:996	catalase	989:996	catalase (CAT)	989:1002	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	8	97	theme	microbiota	1950:1959	arg1	composition					1961:1971	the intestinal microbiota composition	1935:1971	the intestinal microbiota composition	1935:1971	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	8	98	theme	LPS-treated	2039:2049	arg1	mice					2051:2054	LPS-treated mice	2039:2054	LPS-treated mice	2039:2054	CONCLUSION B. pseudolongum CCFM1253 can effectively alleviate inflammatory response and oxidative stress, and regulate the intestinal microbiota composition and liver metabolism, and elevate the liver riboflavin content in LPS-treated mice.
37099000	5	99	theme	pseudolongum	1115:1126	arg1	treatment					1137:1145	B. pseudolongum CCFM1253 treatment	1112:1145	B. pseudolongum CCFM1253 treatment	1112:1145	B. pseudolongum CCFM1253 treatment elevated the proportion of Alistipes and Bifidobacterium, and decreased the proportion of uncultured Bacteroidales bacterium, Muribaculum, Parasutterella, Ruminococcaceae UCG-010 in ALI mice, which were strongly correlated with the inhibition of inflammation responses and oxidative stress.
37099000	6	100	theme	pseudolongum	1519:1530	arg1	CCFM1253					1532:1539	B. pseudolongum CCFM1253	1516:1539	B. pseudolongum CCFM1253	1516:1539	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
37099000	4	101	theme	ALI	1045:1047	arg1	mice					1049:1052	ALI mice	1045:1052	ALI mice	1045:1052	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	0	102	from	injury	164:169	arg1	mice					174:177	mice	174:177	mice	174:177	Intestinal microbiomics and hepatic metabolomics insights into the potential mechanisms of probiotic Bifidobacterium pseudolongum CCFM1253 prevents the acute liver injury in mice.
37099000	3	103	theme	amino	689:693	arg1	transferase					695:705	aspartate amino transferase	679:705	aspartate amino transferase (AST)	679:711	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	3	103	theme	amino	689:693	arg1	AST					708:710	AST	708:710	AST	708:710	RESULTS B. pseudolongum CCFM1253 preintervention remarkably attenuated the influence of LPS on serum alanine transaminase (ALT) and aspartate amino transferase (AST) activities.
37099000	4	104	theme	TNF	839:841	arg1	-α					843:844	[tumor necrosis factor (TNF)-α	815:844	[tumor necrosis factor (TNF)-α	815:844	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	104	theme	TNF	839:841	arg1	T-AOC					953:957	T-AOC	953:957	T-AOC	953:957	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	105	theme	glutathione	1009:1019	arg1	GSH-Px					1033:1038	GSH-Px	1033:1038	GSH-Px	1033:1038	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	105	theme	glutathione	1009:1019	arg1	responses					805:813	the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC)	788:958	the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC)	788:958	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	4	105	theme	glutathione	1009:1019	arg1	peroxidase					1021:1030	glutathione peroxidase	1009:1030	glutathione peroxidase (GSH-Px)]	1009:1040	B. pseudolongum CCFM1253 preintervention remarkably attenuated the inflammation responses [tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6] and elevated antioxidative enzymes activities [total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px)] in ALI mice by intervening the Nf-kβ and Nrf2 pathways, respectively.
37099000	6	106	theme	hepatoprotective	1487:1502	arg1	efficacy					1504:1511	the hepatoprotective efficacy	1483:1511	the hepatoprotective efficacy of B. pseudolongum CCFM1253	1483:1539	Untargeted liver metabolomics exhibited that the hepatoprotective efficacy of B. pseudolongum CCFM1253 might be achieved by altering liver metabolites-related riboflavin metabolism, phenylalanine metabolism, alanine, citrate cycle (TCA cycle), and so on.
36548128	0	0	theme	Cells	82:86	arg1	Protection					52:61	Enhanced Protection	43:61	Enhanced Protection of Human Red Blood Cells Against Freeze-Stress	43:108	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress.
36548128	2	1	theme	human	521:525	arg1	RBCs					527:530	human RBCs	521:530	human RBCs with low hemolysis	521:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	6	2	theme	designed	1221:1228	arg1	glycopeptide					1254:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	2	3	theme	25	392:393	arg1	%					394:394	%	394:394	%	394:394	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	4	4	theme	efficient	1019:1027	arg1	cryopreservation					1029:1044	efficient cryopreservation	1019:1044	efficient cryopreservation	1019:1044	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	1	5	theme	membrane-disruptive	256:274	arg1	activity					276:283	membrane-disruptive activity	256:283	membrane-disruptive activity	256:283	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	0	6	theme	Blood	76:80	arg1	Cells					82:86	Human Red Blood Cells	66:86	Human Red Blood Cells	66:86	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress.
36548128	3	7	theme	dynamic	740:746	arg1	stabilization					776:788	dynamic transition towards membrane stabilization	740:788	dynamic transition towards membrane stabilization	740:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	6	8	theme	alcohol-modified	1237:1252	arg1	glycopeptide					1254:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	3	9	theme	transition	748:757	arg1	stabilization					776:788	dynamic transition towards membrane stabilization	740:788	dynamic transition towards membrane stabilization	740:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	4	10	theme	regulations	944:954	arg1	indicative					887:896	indicative	887:896	indicative	887:896	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	4	10	theme	regulations	944:954	arg1	glycopeptide					871:882	the functionalized glycopeptide	852:882	the functionalized glycopeptide	852:882	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	5	11	theme	recovery	1109:1116	arg1	post-thaw					1118:1126	both high cell recovery post-thaw	1094:1126	both high cell recovery post-thaw	1094:1126	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	5	12	theme	cell	1104:1107	arg1	post-thaw					1118:1126	both high cell recovery post-thaw	1094:1126	both high cell recovery post-thaw	1094:1126	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	3	13	from	surfaces	685:692	arg1	adsorption					622:631	reversible adsorption	611:631	reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization	611:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	6	14	gly	glycopeptide	1254:1265	arg2	glycopeptide					1254:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	3	15	with	perturbation	704:715	arg1	phospholipids					722:734	phospholipids	722:734	phospholipids	722:734	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	3	15	with	perturbation	704:715	arg1	stabilization					776:788	dynamic transition towards membrane stabilization	740:788	dynamic transition towards membrane stabilization	740:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	2	16	theme	dynamic	341:347	arg1	grafting					378:385	a dynamic membrane-active glycopeptide grafting	339:385	a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis	339:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	2	17	from	entry	478:482	arg1	RBCs					527:530	human RBCs	521:530	human RBCs with low hemolysis	521:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	4	18	theme	ice	959:961	arg1	crystals					963:970	ice crystals	959:970	ice crystals	959:970	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	2	19	with	RBCs	527:530	arg1	hemolysis					541:549	low hemolysis	537:549	low hemolysis	537:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	4	20	theme	functionalized	856:869	arg1	indicative					887:896	indicative	887:896	indicative	887:896	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	4	20	theme	functionalized	856:869	arg1	glycopeptide					871:882	the functionalized glycopeptide	852:882	the functionalized glycopeptide	852:882	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	2	21	theme	50	412:413	arg1	%					414:414	%	414:414	%	414:414	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	2	22	theme	p-benzyl	416:423	arg1	alcohol					425:431	50% p-benzyl alcohol	412:431	50% p-benzyl alcohol	412:431	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	2	23	theme	low	537:539	arg1	hemolysis					541:549	low hemolysis	537:549	low hemolysis	537:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	2	24	theme	trehalose	508:516	arg1	entry					478:482	entry	478:482	entry of membrane-impermeable trehalose in human RBCs with low hemolysis	478:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	3	25	theme	mechanism	563:571	arg1	Results					552:558	Results	552:558	Results of mechanism acting on cell membranes	552:596	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	3	26	from	glycopeptide	664:675	arg1	surfaces					685:692	cell surfaces	680:692	cell surfaces	680:692	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	1	27	theme	red	200:202	arg1	RBCs					217:220	RBCs	217:220	RBCs	217:220	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	1	27	theme	red	200:202	arg1	cells					210:214	red blood cells	200:214	red blood cells (RBCs)	200:221	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	0	28	theme	Dynamic	2:8	arg1	Glycopeptide					26:37	A Dynamic Membrane-Active Glycopeptide	0:37	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress	0:108	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress.
36548128	3	29	theme	cellular	816:823	arg1	biofunctions					825:836	cellular biofunctions	816:836	cellular biofunctions	816:836	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	6	30	theme	benzyl	1230:1235	arg1	glycopeptide					1254:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide	1206:1265	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	5	31	theme	all-in-one	1052:1061	arg1	glycopeptide					1063:1074	Such all-in-one glycopeptide	1047:1074	Such all-in-one glycopeptide	1047:1074	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	1	32	theme	blood	204:208	arg1	RBCs					217:220	RBCs	217:220	RBCs	217:220	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	1	32	theme	blood	204:208	arg1	cells					210:214	red blood cells	200:214	red blood cells (RBCs)	200:221	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	1	33	theme	severe	302:307	arg1	hemolysis					309:317	severe hemolysis	302:317	severe hemolysis	302:317	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	4	34	theme	crystals	963:970	arg1	regulations					944:954	typical α-helical/β-sheet structure-driven regulations	901:954	typical α-helical/β-sheet structure-driven regulations of ice crystals	901:970	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	6	35	theme	high	1320:1323	arg1	generality					1325:1334	high generality	1320:1334	high generality	1320:1334	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	5	36	from	%	1174:1174	arg1	protocols					1195:1203	direct freezing protocols	1179:1203	direct freezing protocols	1179:1203	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	1	37	theme	cells	210:214	arg1	cryopreservation					180:195	cryopreservation	180:195	cryopreservation of red blood cells (RBCs)	180:221	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	5	38	theme	exceptional	1142:1152	arg1	cryosurvival					1154:1165	exceptional cryosurvival	1142:1165	exceptional cryosurvival	1142:1165	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	6	39	theme	platform	1306:1313	arg1	development					1279:1289	the development	1275:1289	the development of a competent platform with high generality for protection of blood cells against freeze-stress	1275:1386	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	3	40	theme	cell	583:586	arg1	membranes					588:596	cell membranes	583:596	cell membranes	583:596	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	3	41	from	adsorption	622:631	arg1	surfaces					685:692	cell surfaces	680:692	cell surfaces	680:692	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	2	42	theme	first	441:445	arg1	time					447:450	the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis	437:549	the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis	437:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	0	43	theme	Membrane-Active	10:24	arg1	Glycopeptide					26:37	A Dynamic Membrane-Active Glycopeptide	0:37	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress	0:108	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress.
36548128	1	44	theme	Intracellular	111:123	arg1	delivery					125:132	Intracellular delivery	111:132	Intracellular delivery of freezing-tolerant trehalose	111:163	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	3	45	theme	cell	680:683	arg1	surfaces					685:692	cell surfaces	680:692	cell surfaces	680:692	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	0	46	theme	Enhanced	43:50	arg1	Protection					52:61	Enhanced Protection	43:61	Enhanced Protection of Human Red Blood Cells Against Freeze-Stress	43:108	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress.
36548128	3	47	gly	glycopeptide	664:675	arg2	glycopeptide					664:675	such benzyl alcohol-grafted glycopeptide	636:675	such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization	636:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	3	48	theme	weak	699:702	arg1	perturbation					704:715	weak perturbation	699:715	weak perturbation with phospholipids and dynamic transition towards membrane stabilization	699:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	4	49	theme	α-helical/β-sheet	909:925	arg1	regulations					944:954	typical α-helical/β-sheet structure-driven regulations	901:954	typical α-helical/β-sheet structure-driven regulations of ice crystals	901:970	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	2	50	theme	membrane-impermeable	487:506	arg1	trehalose					508:516	membrane-impermeable trehalose	487:516	membrane-impermeable trehalose	487:516	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	5	51	gly	glycopeptide	1063:1074	arg2	glycopeptide					1063:1074	Such all-in-one glycopeptide	1047:1074	Such all-in-one glycopeptide	1047:1074	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	2	52	with	grafting	378:385	arg1	alcohol					425:431	50% p-benzyl alcohol	412:431	50% p-benzyl alcohol	412:431	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	2	52	with	grafting	378:385	arg1	maltotriose					396:406	25% maltotriose	392:406	25% maltotriose	392:406	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	3	53	theme	such	636:639	arg1	glycopeptide					664:675	such benzyl alcohol-grafted glycopeptide	636:675	such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization	636:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	6	54	with	platform	1306:1313	arg1	generality					1325:1334	high generality	1320:1334	high generality	1320:1334	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	2	55	gly	glycopeptide	365:376	arg2	glycopeptide					365:376	a dynamic membrane-active glycopeptide grafting	339:385	a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis	339:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	3	56	theme	reversible	611:620	arg1	adsorption					622:631	reversible adsorption	611:631	reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization	611:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	5	57	theme	Such	1047:1050	arg1	glycopeptide					1063:1074	Such all-in-one glycopeptide	1047:1074	Such all-in-one glycopeptide	1047:1074	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	5	58	theme	direct	1179:1184	arg1	protocols					1195:1203	direct freezing protocols	1179:1203	direct freezing protocols	1179:1203	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	4	59	theme	structure-driven	927:942	arg1	regulations					944:954	typical α-helical/β-sheet structure-driven regulations	901:954	typical α-helical/β-sheet structure-driven regulations of ice crystals	901:970	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	2	60	theme	glycopeptide	365:376	arg1	grafting					378:385	a dynamic membrane-active glycopeptide grafting	339:385	a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis	339:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	6	61	theme	cells	1360:1364	arg1	protection					1340:1349	protection	1340:1349	protection of blood cells against freeze-stress	1340:1386	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	5	62	theme	freezing	1186:1193	arg1	protocols					1195:1203	direct freezing protocols	1179:1203	direct freezing protocols	1179:1203	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	3	63	theme	glycopeptide	664:675	arg1	adsorption					622:631	reversible adsorption	611:631	reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization	611:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	4	64	theme	typical	901:907	arg1	regulations					944:954	typical α-helical/β-sheet structure-driven regulations	901:954	typical α-helical/β-sheet structure-driven regulations of ice crystals	901:970	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	1	65	theme	freezing-tolerant	137:153	arg1	trehalose					155:163	freezing-tolerant trehalose	137:163	freezing-tolerant trehalose	137:163	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	5	66	theme	high	1099:1102	arg1	post-thaw					1118:1126	both high cell recovery post-thaw	1094:1126	both high cell recovery post-thaw	1094:1126	Such all-in-one glycopeptide enables achieving both high cell recovery post-thaw above 85% and exceptional cryosurvival over 95% in direct freezing protocols.
36548128	1	67	theme	previous	227:234	arg1	strategies					236:245	previous strategies	227:245	previous strategies based on membrane-disruptive activity	227:283	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	6	68	theme	blood	1354:1358	arg1	cells					1360:1364	blood cells	1354:1364	blood cells	1354:1364	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	1	69	theme	trehalose	155:163	arg1	delivery					125:132	Intracellular delivery	111:132	Intracellular delivery of freezing-tolerant trehalose	111:163	Intracellular delivery of freezing-tolerant trehalose is crucial for cryopreservation of red blood cells (RBCs) and previous strategies based on membrane-disruptive activity usually generate severe hemolysis.
36548128	6	70	theme	competent	1296:1304	arg1	platform					1306:1313	a competent platform	1294:1313	a competent platform with high generality for protection of blood cells against freeze-stress	1294:1386	The rationally designed benzyl alcohol-modified glycopeptide permits the development of a competent platform with high generality for protection of blood cells against freeze-stress.
36548128	2	71	theme	membrane-active	349:363	arg1	grafting					378:385	a dynamic membrane-active glycopeptide grafting	339:385	a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis	339:549	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	0	72	theme	Red	72:74	arg1	Cells					82:86	Human Red Blood Cells	66:86	Human Red Blood Cells	66:86	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress.
36548128	3	73	theme	benzyl	641:646	arg1	glycopeptide					664:675	such benzyl alcohol-grafted glycopeptide	636:675	such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization	636:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	2	74	theme	%	414:414	arg1	alcohol					425:431	50% p-benzyl alcohol	412:431	50% p-benzyl alcohol	412:431	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
36548128	4	75	gly	glycopeptide	871:882	arg2	glycopeptide					871:882	the functionalized glycopeptide	852:882	the functionalized glycopeptide	852:882	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	4	75	gly	glycopeptide	871:882	arg2	indicative					887:896	indicative	887:896	indicative	887:896	Furthermore, the functionalized glycopeptide is indicative of typical α-helical/β-sheet structure-driven regulations of ice crystals during freeze-thaw, thereby strongly promoting efficient cryopreservation.
36548128	0	76	theme	Human	66:70	arg1	Cells					82:86	Human Red Blood Cells	66:86	Human Red Blood Cells	66:86	A Dynamic Membrane-Active Glycopeptide for Enhanced Protection of Human Red Blood Cells Against Freeze-Stress.
36548128	3	77	theme	towards	759:765	arg1	stabilization					776:788	dynamic transition towards membrane stabilization	740:788	dynamic transition towards membrane stabilization	740:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	3	78	theme	alcohol-grafted	648:662	arg1	glycopeptide					664:675	such benzyl alcohol-grafted glycopeptide	636:675	such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization	636:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	3	79	theme	membrane	767:774	arg1	stabilization					776:788	dynamic transition towards membrane stabilization	740:788	dynamic transition towards membrane stabilization	740:788	Results of mechanism acting on cell membranes suggest that reversible adsorption of such benzyl alcohol-grafted glycopeptide on cell surfaces upon weak perturbation with phospholipids and dynamic transition towards membrane stabilization are essential for keeping cellular biofunctions.
36548128	2	80	theme	%	394:394	arg1	maltotriose					396:406	25% maltotriose	392:406	25% maltotriose	392:406	Herein, we develop a dynamic membrane-active glycopeptide grafting with 25% maltotriose and 50% p-benzyl alcohol for the first time to effectively facilitate entry of membrane-impermeable trehalose in human RBCs with low hemolysis.
35782137	5	0	theme	type	616:619	arg1	structures					621:630	Lewis type structures	610:630	Lewis type structures	610:630	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	0	theme	type	616:619	arg1	acid					694:697	α2,3-linked sialic acid	675:697	α2,3-linked sialic acid	675:697	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	0	theme	type	616:619	arg1	b/y					649:651	Lewis b/y	643:651	Lewis b/y	643:651	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	0	theme	type	616:619	arg1	a/x					667:669	sialyl-Lewis a/x	654:669	sialyl-Lewis a/x	654:669	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	8	1	theme	x.	909:910	arg1	Binding					912:918	x. Binding	909:918	x. Binding	909:918	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	11	2	theme	Efficient	1191:1199	arg1	binding					1201:1207	Efficient binding	1191:1207	Efficient binding of S. oralis	1191:1220	Efficient binding of S. oralis subsp.
35782137	5	3	theme	structures	621:630	arg1	abundance					597:605	a higher relative abundance	579:605	a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid,	579:698	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	2	4	theme	isogenic	351:358	arg1	mutants					360:366	isogenic mutants	351:366	isogenic mutants	351:366	Here, we investigated the mechanisms of S. oralis binding to oral mucins using clinical isolates, isogenic mutants and glycoconjugates.
35782137	11	5	theme	oralis	1215:1220	arg1	binding					1201:1207	Efficient binding	1191:1207	Efficient binding of S. oralis	1191:1220	Efficient binding of S. oralis subsp.
35782137	8	6	theme	x	982:982	arg1	conjugates					984:993	Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	941:993	Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	941:993	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	2	7	theme	oralis	296:301	arg1	mechanisms					279:288	the mechanisms	275:288	the mechanisms of S. oralis binding to oral mucins	275:324	Here, we investigated the mechanisms of S. oralis binding to oral mucins using clinical isolates, isogenic mutants and glycoconjugates.
35782137	12	8	theme	LPXTG-containing	1327:1342	arg1	s					1360:1360	LPXTG-containing surface protein(s)	1327:1361	LPXTG-containing surface protein(s)	1327:1361	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	12	8	theme	LPXTG-containing	1327:1342	arg1	s					1320:1320	the adhesin(s)	1308:1321	the adhesin(s)	1308:1321	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	8	9	theme	Lewis	976:980	arg1	x					982:982	Lewis x	976:982	Lewis x	976:982	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	1	10	dep	commensal	136:144	arg1	an					128:129	an	128:129	an	128:129	Streptococcus oralis is an oral commensal and opportunistic pathogen that can enter the bloodstream and cause bacteremia and infective endocarditis.
35782137	7	11	theme	Lacto-N-tetraose	802:817	arg1	glycoconjugates					819:833	Lacto-N-tetraose glycoconjugates	802:833	Lacto-N-tetraose glycoconjugates	802:833	oralis binding to MUC5B and MUC7/SAG was inhibited by Lewis b and Lacto-N-tetraose glycoconjugates.
35782137	5	12	theme	relative	588:595	arg1	abundance					597:605	a higher relative abundance	579:605	a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid,	579:698	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	13	theme	higher	581:586	arg1	abundance					597:605	a higher relative abundance	579:605	a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid,	579:698	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	10	14	theme	MUC5B	1168:1172	arg1	mechanisms					1146:1155	the mechanisms	1142:1155	the mechanisms of binding MUC5B and MUC7	1142:1181	oralis binding to oral mucins and that the mechanisms of binding MUC5B and MUC7 differ.
35782137	4	15	theme	Mass	469:472	arg1	spectrometry					474:485	Mass spectrometry	469:485	Mass spectrometry	469:485	Mass spectrometry identified 128 glycans on MUC5B, MUC7 and the salivary agglutinin (SAG).
35782137	12	16	theme	surface	1344:1350	arg1	s					1360:1360	LPXTG-containing surface protein(s)	1327:1361	LPXTG-containing surface protein(s)	1327:1361	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	12	16	theme	surface	1344:1350	arg1	s					1320:1320	the adhesin(s)	1308:1321	the adhesin(s)	1308:1321	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	8	17	theme	Lacto-N-fucopentaose	941:960	arg1	conjugates					984:993	Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	941:993	Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	941:993	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	9	18	theme	distinct	1026:1033	arg1	specificities					1056:1068	three distinct carbohydrate binding specificities	1020:1068	three distinct carbohydrate binding specificities	1020:1068	These data suggest that three distinct carbohydrate binding specificities are involved in S. oralis subsp.
35782137	5	19	contain	contained	569:577	arg2	abundance					597:605	a higher relative abundance	579:605	a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid,	579:698	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	19	contain	contained	569:577	arg1	MUC7/SAG					560:567	MUC7/SAG	560:567	MUC7/SAG	560:567	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	0	20	theme	Streptococcus	0:12	arg1	oralis					14:19	Streptococcus oralis	0:19	Streptococcus oralis	0:19	Streptococcus oralis Employs Multiple Mechanisms of Salivary Mucin Binding That Differ Between Strains.
35782137	5	21	theme	Lewis	610:614	arg1	structures					621:630	Lewis type structures	610:630	Lewis type structures	610:630	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	21	theme	Lewis	610:614	arg1	acid					694:697	α2,3-linked sialic acid	675:697	α2,3-linked sialic acid	675:697	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	21	theme	Lewis	610:614	arg1	b/y					649:651	Lewis b/y	643:651	Lewis b/y	643:651	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	5	21	theme	Lewis	610:614	arg1	a/x					667:669	sialyl-Lewis a/x	654:669	sialyl-Lewis a/x	654:669	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	8	22	theme	type	965:968	arg1	conjugates					984:993	Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	941:993	Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	941:993	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	2	23	theme	oral	314:317	arg1	mucins					319:324	oral mucins	314:324	oral mucins	314:324	Here, we investigated the mechanisms of S. oralis binding to oral mucins using clinical isolates, isogenic mutants and glycoconjugates.
35782137	9	24	theme	carbohydrate	1035:1046	arg1	specificities					1056:1068	three distinct carbohydrate binding specificities	1020:1068	three distinct carbohydrate binding specificities	1020:1068	These data suggest that three distinct carbohydrate binding specificities are involved in S. oralis subsp.
35782137	13	25	theme	adhesins	1417:1424	arg1	AsaA					1461:1464	the sialic acid binding protein AsaA	1429:1464	the sialic acid binding protein AsaA	1429:1464	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	25	theme	adhesins	1417:1424	arg1	one					1404:1406	one	1404:1406	one	1404:1406	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	25	theme	adhesins	1417:1424	arg1	adhesins					1417:1424	these adhesins	1411:1424	these adhesins	1411:1424	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	0	26	theme	Multiple	29:36	arg1	Mechanisms					38:47	Multiple Mechanisms	29:47	Multiple Mechanisms of Salivary Mucin Binding That Differ Between Strains	29:101	Streptococcus oralis Employs Multiple Mechanisms of Salivary Mucin Binding That Differ Between Strains.
35782137	12	27	theme	adhesin	1312:1318	arg1	s					1360:1360	LPXTG-containing surface protein(s)	1327:1361	LPXTG-containing surface protein(s)	1327:1361	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	12	27	theme	adhesin	1312:1318	arg1	s					1320:1320	the adhesin(s)	1308:1321	the adhesin(s)	1308:1321	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	8	28	theme	H	963:963	arg1	type					965:968	H type 2	963:970	H type 2	963:970	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	1	29	theme	Streptococcus	104:116	arg1	oralis					118:123	Streptococcus oralis	104:123	Streptococcus oralis	104:123	Streptococcus oralis is an oral commensal and opportunistic pathogen that can enter the bloodstream and cause bacteremia and infective endocarditis.
35782137	7	30	theme	Lewis	790:794	arg1	b					796:796	Lewis b	790:796	Lewis b	790:796	oralis binding to MUC5B and MUC7/SAG was inhibited by Lewis b and Lacto-N-tetraose glycoconjugates.
35782137	13	31	theme	Further	1364:1370	arg1	investigation					1372:1384	Further investigation	1364:1384	Further investigation	1364:1384	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	3	32	theme	binding	452:458	arg1	level					443:447	a higher level	434:447	a higher level of binding to MUC7	434:466	S. oralis bound to both MUC5B and MUC7, with a higher level of binding to MUC7.
35782137	4	33	theme	salivary	533:540	arg1	SAG					554:556	SAG	554:556	SAG	554:556	Mass spectrometry identified 128 glycans on MUC5B, MUC7 and the salivary agglutinin (SAG).
35782137	4	33	theme	salivary	533:540	arg1	agglutinin					542:551	the salivary agglutinin	529:551	the salivary agglutinin (SAG)	529:557	Mass spectrometry identified 128 glycans on MUC5B, MUC7 and the salivary agglutinin (SAG).
35782137	5	34	theme	α2,3-linked	675:685	arg1	acid					694:697	α2,3-linked sialic acid	675:697	α2,3-linked sialic acid	675:697	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	13	35	theme	binding	1445:1451	arg1	AsaA					1461:1464	the sialic acid binding protein AsaA	1429:1464	the sialic acid binding protein AsaA	1429:1464	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	35	theme	binding	1445:1451	arg1	one					1404:1406	one	1404:1406	one	1404:1406	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	35	theme	binding	1445:1451	arg1	adhesins					1417:1424	these adhesins	1411:1424	these adhesins	1411:1424	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	3	36	theme	higher	436:441	arg1	level					443:447	a higher level	434:447	a higher level of binding to MUC7	434:466	S. oralis bound to both MUC5B and MUC7, with a higher level of binding to MUC7.
35782137	7	37	theme	oralis	736:741	arg1	binding					743:749	oralis binding	736:749	oralis binding to MUC5B and MUC7/SAG	736:771	oralis binding to MUC5B and MUC7/SAG was inhibited by Lewis b and Lacto-N-tetraose glycoconjugates.
35782137	5	38	theme	sialic	687:692	arg1	acid					694:697	α2,3-linked sialic acid	675:697	α2,3-linked sialic acid	675:697	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	13	39	theme	protein	1453:1459	arg1	AsaA					1461:1464	the sialic acid binding protein AsaA	1429:1464	the sialic acid binding protein AsaA	1429:1464	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	39	theme	protein	1453:1459	arg1	one					1404:1406	one	1404:1406	one	1404:1406	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	39	theme	protein	1453:1459	arg1	adhesins					1417:1424	these adhesins	1411:1424	these adhesins	1411:1424	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	0	40	theme	Mucin	61:65	arg1	Binding					67:73	Salivary Mucin Binding	52:73	Salivary Mucin Binding	52:73	Streptococcus oralis Employs Multiple Mechanisms of Salivary Mucin Binding That Differ Between Strains.
35782137	5	41	theme	Lewis	643:647	arg1	b/y					649:651	Lewis b/y	643:651	Lewis b/y	643:651	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	12	42	theme	protein	1352:1358	arg1	s					1360:1360	LPXTG-containing surface protein(s)	1327:1361	LPXTG-containing surface protein(s)	1327:1361	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	12	42	theme	protein	1352:1358	arg1	s					1320:1320	the adhesin(s)	1308:1321	the adhesin(s)	1308:1321	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	0	43	theme	Salivary	52:59	arg1	Binding					67:73	Salivary Mucin Binding	52:73	Salivary Mucin Binding	52:73	Streptococcus oralis Employs Multiple Mechanisms of Salivary Mucin Binding That Differ Between Strains.
35782137	12	44	theme	sortase	1281:1287	arg1	A					1289:1289	sortase A	1281:1289	sortase A	1281:1289	oralis to MUC5B and MUC7 required the gene encoding sortase A, suggesting that the adhesin(s) are LPXTG-containing surface protein(s).
35782137	9	45	theme	S.	1086:1087	arg1	subsp					1096:1100	S. oralis subsp	1086:1100	S. oralis subsp	1086:1100	These data suggest that three distinct carbohydrate binding specificities are involved in S. oralis subsp.
35782137	10	46	theme	MUC7	1178:1181	arg1	mechanisms					1146:1155	the mechanisms	1142:1155	the mechanisms of binding MUC5B and MUC7	1142:1181	oralis binding to oral mucins and that the mechanisms of binding MUC5B and MUC7 differ.
35782137	9	47	theme	oralis	1089:1094	arg1	subsp					1096:1100	S. oralis subsp	1086:1100	S. oralis subsp	1086:1100	These data suggest that three distinct carbohydrate binding specificities are involved in S. oralis subsp.
35782137	1	48	theme	oral	131:134	arg1	commensal					136:144	oral commensal	131:144	oral commensal	131:144	Streptococcus oralis is an oral commensal and opportunistic pathogen that can enter the bloodstream and cause bacteremia and infective endocarditis.
35782137	1	49	theme	infective	229:237	arg1	endocarditis					239:250	infective endocarditis	229:250	infective endocarditis	229:250	Streptococcus oralis is an oral commensal and opportunistic pathogen that can enter the bloodstream and cause bacteremia and infective endocarditis.
35782137	5	50	theme	sialyl-Lewis	654:665	arg1	a/x					667:669	sialyl-Lewis a/x	654:669	sialyl-Lewis a/x	654:669	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	9	51	theme	binding	1048:1054	arg1	specificities					1056:1068	three distinct carbohydrate binding specificities	1020:1068	three distinct carbohydrate binding specificities	1020:1068	These data suggest that three distinct carbohydrate binding specificities are involved in S. oralis subsp.
35782137	8	52	dep	Lewis	903:907	arg1	inhibited					928:936	inhibited	928:936	was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	920:993	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	2	53	theme	clinical	332:339	arg1	isolates					341:348	clinical isolates	332:348	clinical isolates	332:348	Here, we investigated the mechanisms of S. oralis binding to oral mucins using clinical isolates, isogenic mutants and glycoconjugates.
35782137	10	54	dep	mucins	1126:1131	arg1	differ					1183:1188	differ	1183:1188	differ	1183:1188	oralis binding to oral mucins and that the mechanisms of binding MUC5B and MUC7 differ.
35782137	13	55	theme	sialic	1433:1438	arg1	AsaA					1461:1464	the sialic acid binding protein AsaA	1429:1464	the sialic acid binding protein AsaA	1429:1464	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	55	theme	sialic	1433:1438	arg1	one					1404:1406	one	1404:1406	one	1404:1406	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	55	theme	sialic	1433:1438	arg1	adhesins					1417:1424	these adhesins	1411:1424	these adhesins	1411:1424	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	8	56	theme	sialyl	896:901	arg1	Lewis					903:907	sialyl Lewis	896:907	sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates	896:993	In addition, S. oralis binding to MUC7/SAG was inhibited by sialyl Lewis x. Binding was not inhibited by Lacto-N-fucopentaose, H type 2 and Lewis x conjugates.
35782137	5	57	link	α2,3-linked	675:685	arg1	acid					694:697	α2,3-linked sialic acid	675:697	α2,3-linked sialic acid	675:697	MUC7/SAG contained a higher relative abundance of Lewis type structures, including Lewis b/y, sialyl-Lewis a/x and α2,3-linked sialic acid, compared to MUC5B.
35782137	10	58	theme	binding	1160:1166	arg1	MUC5B					1168:1172	binding MUC5B	1160:1172	binding MUC5B	1160:1172	oralis binding to oral mucins and that the mechanisms of binding MUC5B and MUC7 differ.
35782137	10	59	theme	oral	1121:1124	arg1	mucins					1126:1131	oral mucins	1121:1131	oral mucins and that the mechanisms of binding MUC5B and MUC7 differ	1121:1188	oralis binding to oral mucins and that the mechanisms of binding MUC5B and MUC7 differ.
35782137	0	60	theme	Binding	67:73	arg1	Mechanisms					38:47	Multiple Mechanisms	29:47	Multiple Mechanisms of Salivary Mucin Binding That Differ Between Strains	29:101	Streptococcus oralis Employs Multiple Mechanisms of Salivary Mucin Binding That Differ Between Strains.
35782137	13	61	theme	acid	1440:1443	arg1	AsaA					1461:1464	the sialic acid binding protein AsaA	1429:1464	the sialic acid binding protein AsaA	1429:1464	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	61	theme	acid	1440:1443	arg1	one					1404:1406	one	1404:1406	one	1404:1406	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	13	61	theme	acid	1440:1443	arg1	adhesins					1417:1424	these adhesins	1411:1424	these adhesins	1411:1424	Further investigation demonstrated that one of these adhesins is the sialic acid binding protein AsaA.
35782137	1	62	theme	opportunistic	150:162	arg1	pathogen					164:171	opportunistic pathogen	150:171	opportunistic pathogen	150:171	Streptococcus oralis is an oral commensal and opportunistic pathogen that can enter the bloodstream and cause bacteremia and infective endocarditis.
36442762	6	0	theme	water	858:862	arg1	content					864:870	fecal water content	852:870	fecal water content	852:870	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	9	1	theme	fecal	1481:1485	arg1	transplantation					1498:1512	fecal microbiota transplantation	1481:1512	fecal microbiota transplantation (FMT)	1481:1518	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	9	1	theme	fecal	1481:1485	arg1	FMT					1515:1517	FMT	1515:1517	FMT	1515:1517	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	11	2	theme	content	1892:1898	arg1	increase					1875:1882	the increase	1871:1882	the increase of SCFA content in mice with SDD	1871:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	10	3	theme	cup	1733:1735	arg1	cells					1737:1741	colonic cup cells	1725:1741	colonic cup cells	1725:1741	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	6	4	theme	EEDAR	763:767	arg1	effects					752:758	The effects	748:758	The effects of EEDAR	748:767	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	3	5	from	diarrhea	308:315	arg1	China					326:330	China	326:330	China	326:330	is a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years.
36442762	3	6	used	used	276:279	arg2	herb					248:251	a Chinese herb	238:251	a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years	238:356	is a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years.
36442762	6	7	theme	animal	837:842	arg1	weight					844:849	the animal weight	833:849	the animal weight	833:849	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	4	8	theme	antidiarrheal	400:412	arg1	activity					414:421	its antidiarrheal activity	396:421	its antidiarrheal activity	396:421	However, the underlying mechanism of its antidiarrheal activity is not fully understood.
36442762	7	9	dep	factors	984:990	arg1	-α					1020:1021	tumor necrosis factor [TNF]-α	993:1021	tumor necrosis factor [TNF]-α	993:1021	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	9	dep	factors	984:990	arg1	[IL					1036:1038	interleukin [IL]-1β	1024:1042	interleukin [IL]-1β	1024:1042	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	9	dep	factors	984:990	arg1	IL-10					1051:1055	IL-10	1051:1055	IL-10	1051:1055	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	9	dep	factors	984:990	arg1	IL-6					1045:1048	IL-6	1045:1048	IL-6	1045:1048	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	5	10	theme	ethanol	498:504	arg1	extract					506:512	the ethanol extract	494:512	the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE)	494:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	5	11	theme	STUDY	459:463	arg1	AIM					448:450	AIM	448:450	AIM OF THE STUDY	448:463	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	10	12	with	Treatment	1625:1633	arg1	EEDAR					1640:1644	EEDAR	1640:1644	EEDAR	1640:1644	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	3	13	theme	spleen-deficiency	290:306	arg1	SDD					318:320	SDD	318:320	SDD	318:320	is a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years.
36442762	3	13	theme	spleen-deficiency	290:306	arg1	diarrhea					308:315	spleen-deficiency diarrhea	290:315	spleen-deficiency diarrhea (SDD) in China	290:330	is a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years.
36442762	4	14	theme	underlying	372:381	arg1	mechanism					383:391	the underlying mechanism	368:391	the underlying mechanism of its antidiarrheal activity	368:421	However, the underlying mechanism of its antidiarrheal activity is not fully understood.
36442762	7	15	theme	factor	1008:1013	arg1	-α					1020:1021	tumor necrosis factor [TNF]-α	993:1021	tumor necrosis factor [TNF]-α	993:1021	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	15	theme	factor	1008:1013	arg1	[IL					1036:1038	interleukin [IL]-1β	1024:1042	interleukin [IL]-1β	1024:1042	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	15	theme	factor	1008:1013	arg1	IL-10					1051:1055	IL-10	1051:1055	IL-10	1051:1055	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	15	theme	factor	1008:1013	arg1	IL-6					1045:1048	IL-6	1045:1048	IL-6	1045:1048	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	5	16	theme	antidiarrheal	469:481	arg1	effects					483:489	The antidiarrheal effects	465:489	The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE)	465:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	13	17	theme	CONCLUSION	2106:2115	arg1	EEDAR					2117:2121	CONCLUSION EEDAR	2106:2121	CONCLUSION EEDAR	2106:2121	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	6	18	theme	goblet	896:901	arg1	loss					908:911	goblet cell loss	896:911	goblet cell loss	896:911	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	11	19	theme	EEDAR	1862:1866	arg1	related					1929:1935	related	1929:1935	related	1929:1935	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	11	19	theme	EEDAR	1862:1866	arg1	effect					1852:1857	the effect	1848:1857	the effect of EEDAR on the increase of SCFA content in mice with SDD	1848:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	7	20	theme	western	1231:1237	arg1	blotting					1239:1246	western blotting	1231:1246	western blotting	1231:1246	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	10	21	theme	junction	1812:1819	arg1	markers					1821:1827	tight junction markers	1806:1827	tight junction markers	1806:1827	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	0	22	theme	intestinal	153:162	arg1	flora					164:168	the intestinal flora	149:168	the intestinal flora	149:168	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	11	23	theme	gut	1944:1946	arg1	composition					1959:1969	the gut microbiota composition	1940:1969	the gut microbiota composition	1940:1969	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	10	24	theme	AQPs	1797:1800	arg1	expression					1783:1792	the expression	1779:1792	the expression of AQPs and tight junction markers	1779:1827	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	9	25	theme	intestinal	1552:1561	arg1	flora					1563:1567	the intestinal flora	1548:1567	the intestinal flora	1548:1567	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	9	25	theme	intestinal	1552:1561	arg1	essential					1573:1581	essential	1573:1581	essential	1573:1581	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	7	26	theme	factors	984:990	arg1	expression					957:966	The expression	953:966	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues	953:1164	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	27	theme	polymerase	1201:1210	arg1	reaction					1218:1225	quantitative polymerase chain reaction	1188:1225	quantitative polymerase chain reaction	1188:1225	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	10	28	dep	RESULTS	1617:1623	arg1	improved					1660:1667	improved	1660:1667	improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers	1660:1827	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	5	29	theme	fatty	662:666	arg1	SCFA					674:677	SCFA	674:677	SCFA	674:677	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	5	29	theme	fatty	662:666	arg1	acid					668:671	the short-chain fatty acid	646:671	the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora	646:723	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	9	30	from	effect	1424:1429	arg1	flora					1458:1462	the intestinal flora	1443:1462	the intestinal flora of SDD mice	1443:1474	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	8	31	theme	gas	1327:1329	arg1	spectrometry					1351:1362	gas chromatography-mass spectrometry	1327:1362	gas chromatography-mass spectrometry	1327:1362	SCFA metabonomics in the feces of mice treated with EEDAR was evaluated using gas chromatography-mass spectrometry.
36442762	11	32	from	content	1892:1898	arg1	mice					1903:1906	mice	1903:1906	mice with SDD	1903:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	0	33	theme	short-chain	85:95	arg1	acid					103:106	short-chain fatty acid	85:106	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice	50:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	5	34	theme	A.	528:529	arg1	lancea					531:536	deep-fried A. lancea	517:536	deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE)	517:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	1	35	theme	ETHNOPHARMACOLOGICAL	171:190	arg1	lancea					215:220	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea	171:220	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea (Thunb.)	171:229	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea (Thunb.)
36442762	9	36	theme	anti-SDD	1591:1598	arg1	effect					1600:1605	the anti-SDD effect	1587:1605	the anti-SDD effect of EEDAR	1587:1614	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	0	37	theme	acid	103:106	arg1	disorder					118:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder	50:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice	50:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	1	38	theme	Atractylodes	202:213	arg1	lancea					215:220	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea	171:220	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea (Thunb.)	171:229	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea (Thunb.)
36442762	7	39	dep	aquaporins	1059:1068	arg1	AQP4					1077:1080	AQP4	1077:1080	AQP4	1077:1080	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	39	dep	aquaporins	1059:1068	arg1	AQP8					1087:1090	AQP8	1087:1090	AQP8	1087:1090	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	39	dep	aquaporins	1059:1068	arg1	AQP3					1071:1074	AQP3	1071:1074	AQP3	1071:1074	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	39	dep	aquaporins	1059:1068	arg1	aquaporins					1059:1068	aquaporins	1059:1068	aquaporins (AQP3, AQP4, and AQP8)	1059:1091	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	5	40	theme	folium	590:595	arg1	SE					605:606	SE	605:606	SE	605:606	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	5	40	theme	folium	590:595	arg1	sennae					597:602	folium sennae	590:602	folium sennae (SE)	590:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	3	41	theme	thousand	343:350	arg1	years					352:356	a thousand years	341:356	a thousand years	341:356	is a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years.
36442762	12	42	theme	germ-free	2063:2071	arg1	mice					2077:2080	germ-free SDD mice	2063:2080	germ-free SDD mice	2063:2080	EEDAR intervention did not significantly improve intestinal inflammation or the barrier of germ-free SDD mice, but FMT was effective.
36442762	5	43	theme	due	554:556	arg1	lancea					531:536	deep-fried A. lancea	517:536	deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE)	517:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	13	44	theme	microbiota	2241:2250	arg1	imbalance					2213:2221	the imbalance	2209:2221	the imbalance of the intestinal microbiota	2209:2250	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	12	45	theme	mice	2077:2080	arg1	inflammation					2032:2043	intestinal inflammation	2021:2043	intestinal inflammation	2021:2043	EEDAR intervention did not significantly improve intestinal inflammation or the barrier of germ-free SDD mice, but FMT was effective.
36442762	12	45	theme	mice	2077:2080	arg1	barrier					2052:2058	the barrier	2048:2058	the barrier of germ-free SDD mice	2048:2080	EEDAR intervention did not significantly improve intestinal inflammation or the barrier of germ-free SDD mice, but FMT was effective.
36442762	0	46	theme	fatty	97:101	arg1	acid					103:106	short-chain fatty acid	85:106	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice	50:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	0	47	theme	Deep-fried	0:9	arg1	rhizome					31:37	Deep-fried Atractylodes lancea rhizome	0:37	Deep-fried Atractylodes lancea rhizome	0:37	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	0	48	from	disorder	118:125	arg1	mice					130:133	mice	130:133	mice	130:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	5	49	theme	spleen	561:566	arg1	deficiency					568:577	spleen deficiency	561:577	spleen deficiency induced by folium sennae (SE)	561:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	5	50	dep	determined	614:623	arg1	AIM					448:450	AIM	448:450	AIM OF THE STUDY	448:463	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	13	51	from	disorder	2186:2193	arg1	mice					2152:2155	mice	2152:2155	mice	2152:2155	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	0	52	theme	lancea	24:29	arg1	rhizome					31:37	Deep-fried Atractylodes lancea rhizome	0:37	Deep-fried Atractylodes lancea rhizome	0:37	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	7	53	dep	markers	1113:1119	arg1	ZO-1					1122:1125	ZO-1	1122:1125	ZO-1	1122:1125	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	53	dep	markers	1113:1119	arg1	claudin-1					1138:1146	claudin-1	1138:1146	claudin-1	1138:1146	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	53	dep	markers	1113:1119	arg1	occludin					1128:1135	occludin	1128:1135	occludin	1128:1135	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	8	54	theme	SCFA	1249:1252	arg1	metabonomics					1254:1265	SCFA metabonomics	1249:1265	SCFA metabonomics in the feces of mice treated with EEDAR	1249:1305	SCFA metabonomics in the feces of mice treated with EEDAR was evaluated using gas chromatography-mass spectrometry.
36442762	9	55	theme	intestinal	1447:1456	arg1	flora					1458:1462	the intestinal flora	1443:1462	the intestinal flora of SDD mice	1443:1474	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	10	56	theme	mice	1685:1688	arg1	symptoms					1673:1680	the symptoms	1669:1680	the symptoms of mice with SDD	1669:1697	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	1	57	dep	lancea	215:220	arg1	Thunb					223:227	Thunb	223:227	Thunb.	223:228	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea (Thunb.)
36442762	0	58	theme	deficiency	57:66	arg1	disorder					118:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder	50:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice	50:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	10	59	with	mice	1685:1688	arg1	SDD					1695:1697	SDD	1695:1697	SDD	1695:1697	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	13	60	theme	induced	2173:2179	arg1	disorder					2186:2193	the induced SCFA disorder	2169:2193	the induced SCFA disorder	2169:2193	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	6	61	theme	mouse	785:789	arg1	model					791:795	a SE-induced mouse model	772:795	a SE-induced mouse model of SDD	772:802	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	9	62	theme	mice	1471:1474	arg1	flora					1458:1462	the intestinal flora	1443:1462	the intestinal flora of SDD mice	1443:1474	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	9	63	theme	rDNA	1382:1385	arg1	sequencing					1387:1396	16S rDNA sequencing	1378:1396	16S rDNA sequencing	1378:1396	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	11	64	from	effect	1852:1857	arg1	increase					1875:1882	the increase	1871:1882	the increase of SCFA content in mice with SDD	1871:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	8	65	theme	mice	1283:1286	arg1	feces					1274:1278	the feces	1270:1278	the feces of mice treated with EEDAR	1270:1305	SCFA metabonomics in the feces of mice treated with EEDAR was evaluated using gas chromatography-mass spectrometry.
36442762	9	66	used	used	1524:1527	arg2	FMT					1515:1517	FMT	1515:1517	FMT	1515:1517	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	9	66	used	used	1524:1527	arg2	transplantation					1498:1512	fecal microbiota transplantation	1481:1512	fecal microbiota transplantation (FMT)	1481:1518	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	7	67	theme	markers	1113:1119	arg1	expression					957:966	The expression	953:966	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues	953:1164	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	6	68	from	content	864:870	arg1	colon					946:950	the colon	942:950	the colon	942:950	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	10	69	theme	cells	1737:1741	arg1	loss					1717:1720	the loss	1713:1720	the loss of colonic cup cells	1713:1741	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	13	70	from	SDD	2145:2147	arg1	mice					2152:2155	mice	2152:2155	mice	2152:2155	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	6	71	theme	fecal	852:856	arg1	content					864:870	fecal water content	852:870	fecal water content	852:870	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	9	72	theme	microbiota	1487:1496	arg1	transplantation					1498:1512	fecal microbiota transplantation	1481:1512	fecal microbiota transplantation (FMT)	1481:1518	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	9	72	theme	microbiota	1487:1496	arg1	FMT					1515:1517	FMT	1515:1517	FMT	1515:1517	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	7	73	theme	tight	1098:1102	arg1	markers					1113:1119	tight junction markers	1098:1119	tight junction markers (ZO-1, occludin, claudin-1)	1098:1147	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	6	74	dep	MATERIALS	726:734	arg1	effects					752:758	The effects	748:758	The effects of EEDAR	748:767	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	10	75	theme	colonic	1725:1731	arg1	cells					1737:1741	colonic cup cells	1725:1741	colonic cup cells	1725:1741	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	7	76	theme	interleukin	1024:1034	arg1	-α					1020:1021	tumor necrosis factor [TNF]-α	993:1021	tumor necrosis factor [TNF]-α	993:1021	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	76	theme	interleukin	1024:1034	arg1	[IL					1036:1038	interleukin [IL]-1β	1024:1042	interleukin [IL]-1β	1024:1042	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	3	77	theme	Chinese	240:246	arg1	herb					248:251	a Chinese herb	238:251	a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years	238:356	is a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years.
36442762	4	78	theme	activity	414:421	arg1	mechanism					383:391	the underlying mechanism	368:391	the underlying mechanism of its antidiarrheal activity	368:421	However, the underlying mechanism of its antidiarrheal activity is not fully understood.
36442762	6	79	from	weight	844:849	arg1	colon					946:950	the colon	942:950	the colon	942:950	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	8	80	from	metabonomics	1254:1265	arg1	feces					1274:1278	the feces	1270:1278	the feces of mice treated with EEDAR	1270:1305	SCFA metabonomics in the feces of mice treated with EEDAR was evaluated using gas chromatography-mass spectrometry.
36442762	13	81	theme	SE-induced	2134:2143	arg1	SDD					2145:2147	SE-induced SDD	2134:2147	SE-induced SDD in mice	2134:2155	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	7	82	theme	[TNF	1015:1018	arg1	-α					1020:1021	tumor necrosis factor [TNF]-α	993:1021	tumor necrosis factor [TNF]-α	993:1021	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	82	theme	[TNF	1015:1018	arg1	[IL					1036:1038	interleukin [IL]-1β	1024:1042	interleukin [IL]-1β	1024:1042	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	82	theme	[TNF	1015:1018	arg1	IL-10					1051:1055	IL-10	1051:1055	IL-10	1051:1055	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	82	theme	[TNF	1015:1018	arg1	IL-6					1045:1048	IL-6	1045:1048	IL-6	1045:1048	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	5	83	theme	extract	506:512	arg1	effects					483:489	The antidiarrheal effects	465:489	The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE)	465:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	7	84	theme	necrosis	999:1006	arg1	-α					1020:1021	tumor necrosis factor [TNF]-α	993:1021	tumor necrosis factor [TNF]-α	993:1021	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	84	theme	necrosis	999:1006	arg1	[IL					1036:1038	interleukin [IL]-1β	1024:1042	interleukin [IL]-1β	1024:1042	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	84	theme	necrosis	999:1006	arg1	IL-10					1051:1055	IL-10	1051:1055	IL-10	1051:1055	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	84	theme	necrosis	999:1006	arg1	IL-6					1045:1048	IL-6	1045:1048	IL-6	1045:1048	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	6	85	theme	cell	903:906	arg1	loss					908:911	goblet cell loss	896:911	goblet cell loss	896:911	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	10	86	theme	tight	1806:1810	arg1	markers					1821:1827	tight junction markers	1806:1827	tight junction markers	1806:1827	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	7	87	theme	inflammatory	971:982	arg1	factors					984:990	inflammatory factors	971:990	inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10)	971:1056	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	6	88	theme	diarrhea-grade	873:886	arg1	rating					888:893	diarrhea-grade rating	873:893	diarrhea-grade rating	873:893	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	5	89	theme	acid	668:671	arg1	metabonomics					680:691	the short-chain fatty acid (SCFA) metabonomics	646:691	the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora	646:723	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	9	90	used	used	1402:1405	arg2	sequencing					1387:1396	16S rDNA sequencing	1378:1396	16S rDNA sequencing	1378:1396	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	5	91	theme	short-chain	650:660	arg1	SCFA					674:677	SCFA	674:677	SCFA	674:677	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	5	91	theme	short-chain	650:660	arg1	acid					668:671	the short-chain fatty acid	646:671	the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora	646:723	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	11	92	theme	SCFA	1887:1890	arg1	content					1892:1898	SCFA content	1887:1898	SCFA content in mice with SDD	1887:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	8	93	theme	chromatography-mass	1331:1349	arg1	spectrometry					1351:1362	gas chromatography-mass spectrometry	1327:1362	gas chromatography-mass spectrometry	1327:1362	SCFA metabonomics in the feces of mice treated with EEDAR was evaluated using gas chromatography-mass spectrometry.
36442762	7	94	theme	chain	1212:1216	arg1	reaction					1218:1225	quantitative polymerase chain reaction	1188:1225	quantitative polymerase chain reaction	1188:1225	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	5	95	theme	intestinal	708:717	arg1	flora					719:723	the intestinal flora	704:723	the intestinal flora	704:723	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	5	96	theme	deep-fried	517:526	arg1	lancea					531:536	deep-fried A. lancea	517:536	deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE)	517:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	9	97	theme	16S	1378:1380	arg1	sequencing					1387:1396	16S rDNA sequencing	1378:1396	16S rDNA sequencing	1378:1396	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	7	98	theme	quantitative	1188:1199	arg1	reaction					1218:1225	quantitative polymerase chain reaction	1188:1225	quantitative polymerase chain reaction	1188:1225	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	10	99	theme	markers	1821:1827	arg1	expression					1783:1792	the expression	1779:1792	the expression of AQPs and tight junction markers	1779:1827	RESULTS Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis, and promoting the expression of AQPs and tight junction markers.
36442762	0	100	theme	metabolic	108:116	arg1	disorder					118:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder	50:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice	50:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	5	101	theme	lancea	531:536	arg1	extract					506:512	the ethanol extract	494:512	the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE)	494:607	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	1	102	theme	RELEVANCE	192:200	arg1	lancea					215:220	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea	171:220	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea (Thunb.)	171:229	ETHNOPHARMACOLOGICAL RELEVANCE Atractylodes lancea (Thunb.)
36442762	5	103	theme	metabonomics	680:691	arg1	regulation					632:641	the regulation	628:641	the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora	628:723	AIM OF THE STUDY The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora.
36442762	6	104	theme	pathological	918:929	arg1	changes					931:937	pathological changes	918:937	pathological changes in the colon	918:950	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	9	105	theme	EEDAR	1610:1614	arg1	effect					1600:1605	the anti-SDD effect	1587:1605	the anti-SDD effect of EEDAR	1587:1614	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	12	106	theme	SDD	2073:2075	arg1	mice					2077:2080	germ-free SDD mice	2063:2080	germ-free SDD mice	2063:2080	EEDAR intervention did not significantly improve intestinal inflammation or the barrier of germ-free SDD mice, but FMT was effective.
36442762	11	107	from	increase	1875:1882	arg1	mice					1903:1906	mice	1903:1906	mice with SDD	1903:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	13	108	theme	intestinal	2230:2239	arg1	microbiota					2241:2250	the intestinal microbiota	2226:2250	the intestinal microbiota	2226:2250	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	0	109	theme	Atractylodes	11:22	arg1	rhizome					31:37	Deep-fried Atractylodes lancea rhizome	0:37	Deep-fried Atractylodes lancea rhizome	0:37	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	7	110	theme	colon	1152:1156	arg1	tissues					1158:1164	colon tissues	1152:1164	colon tissues	1152:1164	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	6	111	from	changes	931:937	arg1	colon					946:950	the colon	942:950	the colon	942:950	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	9	112	theme	EEDAR	1434:1438	arg1	effect					1424:1429	the effect	1420:1429	the effect of EEDAR on the intestinal flora of SDD mice	1420:1474	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	6	113	from	METHODS	740:746	arg1	model					791:795	a SE-induced mouse model	772:795	a SE-induced mouse model of SDD	772:802	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	11	114	theme	microbiota	1948:1957	arg1	composition					1959:1969	the gut microbiota composition	1940:1969	the gut microbiota composition	1940:1969	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	12	115	theme	intestinal	2021:2030	arg1	inflammation					2032:2043	intestinal inflammation	2021:2043	intestinal inflammation	2021:2043	EEDAR intervention did not significantly improve intestinal inflammation or the barrier of germ-free SDD mice, but FMT was effective.
36442762	11	116	with	mice	1903:1906	arg1	SDD					1913:1915	SDD	1913:1915	SDD	1913:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	0	117	theme	spleen	50:55	arg1	disorder					118:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder	50:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice	50:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	6	118	from	loss	908:911	arg1	colon					946:950	the colon	942:950	the colon	942:950	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	12	119	theme	EEDAR	1972:1976	arg1	intervention					1978:1989	EEDAR intervention	1972:1989	EEDAR intervention	1972:1989	EEDAR intervention did not significantly improve intestinal inflammation or the barrier of germ-free SDD mice, but FMT was effective.
36442762	0	120	theme	diarrhea-induced	68:83	arg1	disorder					118:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder	50:125	spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice	50:133	Deep-fried Atractylodes lancea rhizome alleviates spleen deficiency diarrhea-induced short-chain fatty acid metabolic disorder in mice by remodeling the intestinal flora.
36442762	11	121	from	mice	1903:1906	arg1	increase					1875:1882	the increase	1871:1882	the increase of SCFA content in mice with SDD	1871:1915	More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition.
36442762	6	122	theme	SDD	800:802	arg1	model					791:795	a SE-induced mouse model	772:795	a SE-induced mouse model of SDD	772:802	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	13	123	theme	SCFA	2181:2184	arg1	disorder					2186:2193	the induced SCFA disorder	2169:2193	the induced SCFA disorder	2169:2193	CONCLUSION EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
36442762	7	124	theme	tumor	993:997	arg1	-α					1020:1021	tumor necrosis factor [TNF]-α	993:1021	tumor necrosis factor [TNF]-α	993:1021	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	124	theme	tumor	993:997	arg1	[IL					1036:1038	interleukin [IL]-1β	1024:1042	interleukin [IL]-1β	1024:1042	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	124	theme	tumor	993:997	arg1	IL-10					1051:1055	IL-10	1051:1055	IL-10	1051:1055	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	124	theme	tumor	993:997	arg1	IL-6					1045:1048	IL-6	1045:1048	IL-6	1045:1048	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	9	125	theme	SDD	1467:1469	arg1	mice					1471:1474	SDD mice	1467:1474	SDD mice	1467:1474	Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR.
36442762	6	126	from	MATERIALS	726:734	arg1	model					791:795	a SE-induced mouse model	772:795	a SE-induced mouse model of SDD	772:802	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	7	127	from	expression	957:966	arg1	tissues					1158:1164	colon tissues	1152:1164	colon tissues	1152:1164	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	6	128	from	rating	888:893	arg1	colon					946:950	the colon	942:950	the colon	942:950	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	6	129	theme	SE-induced	774:783	arg1	model					791:795	a SE-induced mouse model	772:795	a SE-induced mouse model of SDD	772:802	MATERIALS AND METHODS The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon.
36442762	7	130	theme	junction	1104:1111	arg1	markers					1113:1119	tight junction markers	1098:1119	tight junction markers (ZO-1, occludin, claudin-1)	1098:1147	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
36442762	7	131	theme	aquaporins	1059:1068	arg1	expression					957:966	The expression	953:966	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues	953:1164	The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin, claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting.
35114907	17	0	theme	colonic	2418:2424	arg1	tissue					2426:2431	colonic tissue	2418:2431	colonic tissue	2418:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	4	1	theme	A.	304:305	arg1	SCZ					315:317	SCZ	315:317	SCZ	315:317	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	4	1	theme	A.	304:305	arg1	lancea					307:312	fry raw A. lancea	296:312	people fry raw A. lancea (SCZ) together with wheat bran	289:343	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	9	2	theme	length	1038:1043	arg1	shortening					1045:1054	colon length shortening	1032:1054	colon length shortening	1032:1054	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	2	theme	length	1038:1043	arg1	index					1013:1017	disease activity index	996:1017	disease activity index	996:1017	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	7	3	theme	therapeutic	636:646	arg1	effects					648:654	the therapeutic effects	632:654	the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice	632:741	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	3	4	theme	diseases	214:221	arg1	treatment					184:192	the treatment	180:192	the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea	180:276	is a herb widely used traditionally for the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea.
35114907	13	5	theme	pathological	1780:1791	arg1	indices					1793:1799	pathological indices	1780:1799	pathological indices	1780:1799	Pearson analysis was used to determine the correlation between the intestinal flora, metabolites, and pathological indices.
35114907	19	6	theme	bacteria	2588:2595	arg1	abundance					2564:2572	the abundance	2560:2572	the abundance of beneficial bacteria	2560:2595	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	16	7	theme	changes	2202:2208	arg1	score					2121:2125	the DAI score	2113:2125	the DAI score	2113:2125	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	7	theme	changes	2202:2208	arg1	shortening					2141:2150	colon length shortening	2128:2150	colon length shortening	2128:2150	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	7	theme	changes	2202:2208	arg1	extent					2179:2184	the extent	2175:2184	the extent of pathological changes in the colons of mice with DSS-induced colitis	2175:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	7	theme	changes	2202:2208	arg1	loss					2165:2168	goblet cell loss	2153:2168	goblet cell loss	2153:2168	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	3	8	theme	spleen	246:251	arg1	deficiency					253:262	spleen deficiency	246:262	spleen deficiency	246:262	is a herb widely used traditionally for the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea.
35114907	4	9	theme	A.	364:365	arg1	FCZ					375:377	FCZ	375:377	FCZ	375:377	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	4	9	theme	A.	364:365	arg1	lancea					367:372	bran-fried A. lancea	353:372	bran-fried A. lancea (FCZ)	353:378	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	9	10	theme	cell	1064:1067	arg1	loss					1069:1072	goblet cell loss	1057:1072	goblet cell loss	1057:1072	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	15	11	used	used	1960:1963	arg2	model					1950:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	8	12	theme	reference	891:899	arg1	compounds					901:909	six reference compounds	887:909	six reference compounds	887:909	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	17	13	theme	pro-inflammatory	2386:2401	arg1	macrophages					2403:2413	pro-inflammatory macrophages	2386:2413	pro-inflammatory macrophages	2386:2413	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	16	14	theme	colon	2128:2132	arg1	shortening					2141:2150	colon length shortening	2128:2150	colon length shortening	2128:2150	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	20	15	dep	expression	2747:2756	arg1	PRODH					2767:2771	PRODH	2767:2771	PRODH	2767:2771	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	20	15	dep	expression	2747:2756	arg1	Slc6A7					2759:2764	Slc6A7	2759:2764	Slc6A7	2759:2764	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	20	15	dep	expression	2747:2756	arg1	HMGCR					2780:2784	HMGCR	2780:2784	HMGCR	2780:2784	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	20	15	dep	expression	2747:2756	arg1	SREBP-2					2787:2793	SREBP-2	2787:2793	SREBP-2	2787:2793	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	20	15	dep	expression	2747:2756	arg1	Sdsl					2774:2777	Sdsl	2774:2777	Sdsl	2774:2777	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	6	16	dep	effect	513:518	arg1	the					509:511	the	509:511	the	509:511	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	10	17	theme	necrosis	1229:1236	arg1	factor-[Formula					1238:1252	tumor necrosis factor-[Formula	1223:1252	tumor necrosis factor-[Formula	1223:1252	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	22	18	theme	DSS-induced	2948:2958	arg1	UC					2960:2961	DSS-induced UC	2948:2961	DSS-induced UC in mice	2948:2969	Rhizoma Atractylodes was effective in alleviating DSS-induced UC in mice, and FCZ was found to be superior to SCZ.
35114907	7	19	theme	study	611:615	arg1	aim					599:601	The aim	595:601	The aim of this study	595:615	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	22	20	from	UC	2960:2961	arg1	mice					2966:2969	mice	2966:2969	mice	2966:2969	Rhizoma Atractylodes was effective in alleviating DSS-induced UC in mice, and FCZ was found to be superior to SCZ.
35114907	15	21	theme	mouse	1944:1948	arg1	model					1950:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	12	22	from	levels	1648:1653	arg1	group					1671:1675	each group	1666:1675	each group	1666:1675	Nontargeted metabonomics was used to detect the serum metabolite levels of mice in each group.
35114907	19	23	theme	disease-related	2635:2649	arg1	metabolites					2651:2661	disease-related metabolites	2635:2661	disease-related metabolites such as amino acid and cholesterol metabolism	2635:2707	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	19	23	theme	disease-related	2635:2649	arg1	acid					2677:2680	amino acid	2671:2680	amino acid	2671:2680	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	19	23	theme	disease-related	2635:2649	arg1	metabolism					2698:2707	cholesterol metabolism	2686:2707	cholesterol metabolism	2686:2707	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	15	24	theme	PGF	1939:1941	arg1	model					1950:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	9	25	from	model	1130:1134	arg1	colons					1110:1115	the colons	1106:1115	the colons from a mouse model of DSS-induced UC	1106:1152	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	7	26	from	effects	648:654	arg1	UC					732:733	dextran sulfate sodium (DSS)-induced UC	695:733	dextran sulfate sodium (DSS)-induced UC in mice	695:741	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	17	27	theme	macrophages	2403:2413	arg1	activation					2372:2381	activation	2372:2381	activation of pro-inflammatory macrophages in colonic tissue	2372:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	27	theme	macrophages	2403:2413	arg1	occludin					2318:2325	occludin	2318:2325	occludin	2318:2325	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	27	theme	macrophages	2403:2413	arg1	production					2328:2337	production	2328:2337	production of pro-inflammatory factors	2328:2365	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	27	theme	macrophages	2403:2413	arg1	ZO-1					2312:2315	ZO-1	2312:2315	ZO-1	2312:2315	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	27	theme	macrophages	2403:2413	arg1	MUC2					2306:2309	MUC2	2306:2309	MUC2	2306:2309	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	15	28	theme	FCZ	2005:2007	arg1	effect					1987:1992	the effect	1983:1992	the effect of SCZ and FCZ in UC	1983:2013	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	11	29	from	composition	1532:1542	arg1	group					1576:1580	each group	1571:1580	each group	1571:1580	16s RNA sequencing technology was used to detect the composition of the intestinal flora in each group.
35114907	13	30	theme	intestinal	1745:1754	arg1	flora					1756:1760	the intestinal flora	1741:1760	the intestinal flora	1741:1760	Pearson analysis was used to determine the correlation between the intestinal flora, metabolites, and pathological indices.
35114907	10	31	dep	ZO-1	1350:1353	arg1	occludin					1356:1363	occludin	1356:1363	occludin	1356:1363	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	23	32	theme	SCZ	3048:3050	arg1	action					3030:3035	action	3030:3035	action of FCZ and SCZ	3030:3050	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	15	33	theme	SCZ	1997:1999	arg1	effect					1987:1992	the effect	1983:1992	the effect of SCZ and FCZ in UC	1983:2013	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	20	34	theme	metabolite-related	2809:2826	arg1	enzymes					2828:2834	different metabolite-related enzymes	2799:2834	different metabolite-related enzymes	2799:2834	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	1	35	dep	lancea	120:125	arg1	Thunb					128:132	Thunb	128:132	Thunb.	128:133	Atractylodes lancea (Thunb.)
35114907	0	36	theme	Intestinal	73:82	arg1	Flora					84:88	Intestinal Flora	73:88	Intestinal Flora	73:88	Atractylodes lancea Rhizoma Attenuates DSS-Induced Colitis by Regulating Intestinal Flora and Metabolites.
35114907	23	37	theme	FCZ	3040:3042	arg1	action					3030:3035	action	3030:3035	action of FCZ and SCZ	3030:3050	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	19	38	theme	harmful	2529:2535	arg1	bacteria					2537:2544	harmful bacteria	2529:2544	harmful bacteria	2529:2544	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	17	39	theme	pro-inflammatory	2342:2357	arg1	factors					2359:2365	pro-inflammatory factors	2342:2365	pro-inflammatory factors	2342:2365	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	18	40	theme	FCZ	2448:2450	arg1	better					2456:2461	better	2456:2461	better	2456:2461	The effect of FCZ was better than that of SCZ.
35114907	18	40	theme	FCZ	2448:2450	arg1	effect					2438:2443	The effect	2434:2443	The effect of FCZ	2434:2450	The effect of FCZ was better than that of SCZ.
35114907	11	41	theme	flora	1562:1566	arg1	composition					1532:1542	the composition	1528:1542	the composition of the intestinal flora in each group	1528:1580	16s RNA sequencing technology was used to detect the composition of the intestinal flora in each group.
35114907	9	42	theme	DSS-induced	1139:1149	arg1	UC					1151:1152	DSS-induced UC	1139:1152	DSS-induced UC	1139:1152	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	17	43	from	activation	2372:2381	arg1	tissue					2426:2431	colonic tissue	2418:2431	colonic tissue	2418:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	19	44	theme	cholesterol	2686:2696	arg1	metabolism					2698:2707	cholesterol metabolism	2686:2707	cholesterol metabolism	2686:2707	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	19	45	theme	metabolites	2651:2661	arg1	metabolism					2621:2630	the metabolism	2617:2630	the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism	2617:2707	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	13	46	theme	Pearson	1678:1684	arg1	analysis					1686:1693	Pearson analysis	1678:1693	Pearson analysis	1678:1693	Pearson analysis was used to determine the correlation between the intestinal flora, metabolites, and pathological indices.
35114907	14	47	theme	Reverse	1802:1808	arg1	reaction					1841:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction was used to detect the genes of different metabolite-related enzymes.
35114907	8	48	theme	chemical	748:755	arg1	constituents					757:768	The chemical constituents	744:768	The chemical constituents of SCZ and FCZ	744:783	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	14	49	theme	enzymes	1911:1917	arg1	genes					1873:1877	the genes	1869:1877	the genes of different metabolite-related enzymes	1869:1917	Reverse transcription-polymerase chain reaction was used to detect the genes of different metabolite-related enzymes.
35114907	5	50	dep	Chinese	389:395	arg1	texts					397:401	texts	397:401	texts	397:401	Ancient Chinese texts have documented that FCZ can enhance the function of regulating the intestines and stomach.
35114907	14	51	theme	different	1882:1890	arg1	enzymes					1911:1917	different metabolite-related enzymes	1882:1917	different metabolite-related enzymes	1882:1917	Reverse transcription-polymerase chain reaction was used to detect the genes of different metabolite-related enzymes.
35114907	3	52	theme	gastrointestinal	197:212	arg1	diarrhea					269:276	diarrhea	269:276	diarrhea	269:276	is a herb widely used traditionally for the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea.
35114907	3	52	theme	gastrointestinal	197:212	arg1	diseases					214:221	gastrointestinal diseases	197:221	gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea	197:276	is a herb widely used traditionally for the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea.
35114907	3	52	theme	gastrointestinal	197:212	arg1	ulcer					239:243	gastric ulcer	231:243	gastric ulcer	231:243	is a herb widely used traditionally for the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea.
35114907	3	52	theme	gastrointestinal	197:212	arg1	deficiency					253:262	spleen deficiency	246:262	spleen deficiency	246:262	is a herb widely used traditionally for the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea.
35114907	21	53	theme	above	2865:2869	arg1	effects					2871:2877	the above effects	2861:2877	the above effects	2861:2877	In the PGF mouse model, the above effects were not observed.
35114907	0	54	theme	Atractylodes	0:11	arg1	Rhizoma					20:26	Atractylodes lancea Rhizoma	0:26	Atractylodes lancea Rhizoma	0:26	Atractylodes lancea Rhizoma Attenuates DSS-Induced Colitis by Regulating Intestinal Flora and Metabolites.
35114907	1	55	theme	Atractylodes	107:118	arg1	lancea					120:125	Atractylodes lancea	107:125	Atractylodes lancea (Thunb.)	107:134	Atractylodes lancea (Thunb.)
35114907	17	56	from	production	2328:2337	arg1	tissue					2426:2431	colonic tissue	2418:2431	colonic tissue	2418:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	10	57	theme	cytokines	1212:1220	arg1	levels					1185:1190	levels	1185:1190	levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages	1185:1399	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	58	dep	see	1304:1306	arg1	interleukin-1[Formula					1281:1301	interleukin-1[Formula	1281:1301	interleukin-1[Formula	1281:1301	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	16	59	theme	mice	2227:2230	arg1	colons					2217:2222	the colons	2213:2222	the colons of mice with DSS-induced colitis	2213:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	17	60	from	occludin	2318:2325	arg1	tissue					2426:2431	colonic tissue	2418:2431	colonic tissue	2418:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	16	61	from	changes	2202:2208	arg1	colons					2217:2222	the colons	2213:2222	the colons of mice with DSS-induced colitis	2213:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	11	62	theme	sequencing	1487:1496	arg1	technology					1498:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology was used to detect the composition of the intestinal flora in each group.
35114907	9	63	theme	disease	996:1002	arg1	DAI					1020:1022	DAI	1020:1022	DAI	1020:1022	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	63	theme	disease	996:1002	arg1	index					1013:1017	disease activity index	996:1017	disease activity index	996:1017	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	63	theme	disease	996:1002	arg1	shortening					1045:1054	colon length shortening	1032:1054	colon length shortening	1032:1054	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	21	64	theme	mouse	2848:2852	arg1	model					2854:2858	the PGF mouse model	2840:2858	the PGF mouse model	2840:2858	In the PGF mouse model, the above effects were not observed.
35114907	0	65	theme	DSS-Induced	39:49	arg1	Colitis					51:57	DSS-Induced Colitis	39:57	DSS-Induced Colitis	39:57	Atractylodes lancea Rhizoma Attenuates DSS-Induced Colitis by Regulating Intestinal Flora and Metabolites.
35114907	12	66	theme	mice	1658:1661	arg1	levels					1648:1653	the serum metabolite levels	1627:1653	the serum metabolite levels of mice in each group	1627:1675	Nontargeted metabonomics was used to detect the serum metabolite levels of mice in each group.
35114907	16	67	theme	DSS-induced	2237:2247	arg1	colitis					2249:2255	DSS-induced colitis	2237:2255	DSS-induced colitis	2237:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	15	68	theme	free	1933:1936	arg1	model					1950:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	17	69	from	ZO-1	2312:2315	arg1	tissue					2426:2431	colonic tissue	2418:2431	colonic tissue	2418:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	10	70	theme	tight	1335:1339	arg1	ZO-1					1350:1353	ZO-1	1350:1353	ZO-1	1350:1353	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	70	theme	tight	1335:1339	arg1	protein					1341:1347	tight protein	1335:1347	tight protein (ZO-1, occludin)	1335:1364	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	70	theme	tight	1335:1339	arg1	cytokines					1212:1220	the inflammatory cytokines	1195:1220	the inflammatory cytokines (tumor necrosis factor-[Formula: see text]	1195:1263	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	11	71	theme	16s	1479:1481	arg1	technology					1498:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology was used to detect the composition of the intestinal flora in each group.
35114907	10	72	theme	FCZ	1178:1180	arg1	effects					1159:1165	The effects	1155:1165	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages	1155:1399	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	73	dep	interleukin-6	1266:1278	arg1	see					1304:1306	see	1304:1306	see text	1304:1311	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	7	74	theme	FCZ	667:669	arg1	effects					648:654	the therapeutic effects	632:654	the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice	632:741	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	6	75	from	effect	513:518	arg1	colitis					563:569	ulcerative colitis	552:569	ulcerative colitis (UC)	552:574	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	6	75	from	effect	513:518	arg1	UC					572:573	UC	572:573	UC	572:573	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	8	76	theme	liquid	822:827	arg1	HPLC-MS/MS					870:879	HPLC-MS/MS	870:879	HPLC-MS/MS	870:879	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	8	76	theme	liquid	822:827	arg1	spectrometry					856:867	high-performance liquid chromatography-tandem mass spectrometry	805:867	high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds	805:909	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	17	77	from	MUC2	2306:2309	arg1	tissue					2426:2431	colonic tissue	2418:2431	colonic tissue	2418:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	7	78	from	UC	732:733	arg1	mice					738:741	mice	738:741	mice	738:741	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	10	79	theme	SCZ	1170:1172	arg1	effects					1159:1165	The effects	1155:1165	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages	1155:1399	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	16	80	with	mice	2227:2230	arg1	colitis					2249:2255	DSS-induced colitis	2237:2255	DSS-induced colitis	2237:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	7	81	theme	SCZ	659:661	arg1	effects					648:654	the therapeutic effects	632:654	the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice	632:741	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	12	82	theme	serum	1631:1635	arg1	levels					1648:1653	the serum metabolite levels	1627:1653	the serum metabolite levels of mice in each group	1627:1675	Nontargeted metabonomics was used to detect the serum metabolite levels of mice in each group.
35114907	6	83	from	mechanism	524:532	arg1	colitis					563:569	ulcerative colitis	552:569	ulcerative colitis (UC)	552:574	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	6	83	from	mechanism	524:532	arg1	UC					572:573	UC	572:573	UC	572:573	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	8	84	theme	mass	851:854	arg1	HPLC-MS/MS					870:879	HPLC-MS/MS	870:879	HPLC-MS/MS	870:879	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	8	84	theme	mass	851:854	arg1	spectrometry					856:867	high-performance liquid chromatography-tandem mass spectrometry	805:867	high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds	805:909	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	17	85	from	decrease	2294:2301	arg1	production					2328:2337	production	2328:2337	production of pro-inflammatory factors	2328:2365	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	85	from	decrease	2294:2301	arg1	activation					2372:2381	activation	2372:2381	activation of pro-inflammatory macrophages in colonic tissue	2372:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	85	from	decrease	2294:2301	arg1	occludin					2318:2325	occludin	2318:2325	occludin	2318:2325	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	85	from	decrease	2294:2301	arg1	ZO-1					2312:2315	ZO-1	2312:2315	ZO-1	2312:2315	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	85	from	decrease	2294:2301	arg1	MUC2					2306:2309	MUC2	2306:2309	MUC2	2306:2309	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	16	86	theme	cell	2160:2163	arg1	loss					2165:2168	goblet cell loss	2153:2168	goblet cell loss	2153:2168	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	10	87	dep	see	1255:1257	arg1	factor-[Formula					1238:1252	tumor necrosis factor-[Formula	1223:1252	tumor necrosis factor-[Formula	1223:1252	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	23	88	theme	flora	3102:3106	arg1	metabolites					3129:3139	their associated metabolites	3112:3139	their associated metabolites	3112:3139	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	23	88	theme	flora	3102:3106	arg1	regulation					3077:3086	the regulation	3073:3086	the regulation of intestinal flora	3073:3106	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	12	89	theme	Nontargeted	1583:1593	arg1	metabonomics					1595:1606	Nontargeted metabonomics	1583:1606	Nontargeted metabonomics	1583:1606	Nontargeted metabonomics was used to detect the serum metabolite levels of mice in each group.
35114907	6	90	theme	FCZ	545:547	arg1	mechanism					524:532	mechanism	524:532	mechanism	524:532	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	6	90	theme	FCZ	545:547	arg1	effect					513:518	effect	513:518	effect	513:518	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	19	91	theme	bacteria	2537:2544	arg1	abundance					2516:2524	the abundance	2512:2524	the abundance of harmful bacteria	2512:2544	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	9	92	theme	colon	1032:1036	arg1	shortening					1045:1054	colon length shortening	1032:1054	colon length shortening	1032:1054	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	92	theme	colon	1032:1036	arg1	index					1013:1017	disease activity index	996:1017	disease activity index	996:1017	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	10	93	dep	cytokines	1212:1220	arg1	see					1255:1257	see	1255:1257	see text	1255:1262	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	16	94	theme	pathological	2189:2200	arg1	changes					2202:2208	pathological changes	2189:2208	pathological changes in the colons of mice with DSS-induced colitis	2189:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	3	95	theme	gastric	231:237	arg1	ulcer					239:243	gastric ulcer	231:243	gastric ulcer	231:243	is a herb widely used traditionally for the treatment of gastrointestinal diseases such as gastric ulcer, spleen deficiency, and diarrhea.
35114907	4	96	theme	bran-fried	353:362	arg1	FCZ					375:377	FCZ	375:377	FCZ	375:377	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	4	96	theme	bran-fried	353:362	arg1	lancea					367:372	bran-fried A. lancea	353:372	bran-fried A. lancea (FCZ)	353:378	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	19	97	theme	beneficial	2577:2586	arg1	bacteria					2588:2595	beneficial bacteria	2577:2595	beneficial bacteria	2577:2595	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	10	98	theme	tumor	1223:1227	arg1	factor-[Formula					1238:1252	tumor necrosis factor-[Formula	1223:1252	tumor necrosis factor-[Formula	1223:1252	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	15	99	theme	flora	2055:2059	arg1	regulation					2030:2039	the regulation	2026:2039	the regulation of intestinal flora	2026:2059	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	16	100	theme	length	2134:2139	arg1	shortening					2141:2150	colon length shortening	2128:2150	colon length shortening	2128:2150	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	9	101	theme	goblet	1057:1062	arg1	loss					1069:1072	goblet cell loss	1057:1072	goblet cell loss	1057:1072	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	11	102	used	used	1513:1516	arg2	technology					1498:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology was used to detect the composition of the intestinal flora in each group.
35114907	4	103	theme	wheat	334:338	arg1	bran					340:343	wheat bran	334:343	wheat bran	334:343	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	10	104	theme	macrophages	1389:1399	arg1	interleukin-6					1266:1278	interleukin-6	1266:1278	interleukin-6, interleukin-1[Formula: see text]	1266:1312	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	104	theme	macrophages	1389:1399	arg1	mucoprotein					1315:1325	mucoprotein	1315:1325	mucoprotein (MUC2)	1315:1332	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	104	theme	macrophages	1389:1399	arg1	protein					1341:1347	tight protein	1335:1347	tight protein (ZO-1, occludin)	1335:1364	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	104	theme	macrophages	1389:1399	arg1	ZO-1					1350:1353	ZO-1	1350:1353	ZO-1	1350:1353	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	104	theme	macrophages	1389:1399	arg1	cytokines					1212:1220	the inflammatory cytokines	1195:1220	the inflammatory cytokines (tumor necrosis factor-[Formula: see text]	1195:1263	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	104	theme	macrophages	1389:1399	arg1	activation					1375:1384	the activation	1371:1384	the activation of macrophages	1371:1399	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	104	theme	macrophages	1389:1399	arg1	MUC2					1328:1331	MUC2	1328:1331	MUC2	1328:1331	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	16	105	theme	DAI	2117:2119	arg1	score					2121:2125	the DAI score	2113:2125	the DAI score	2113:2125	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	106	from	shortening	2141:2150	arg1	colons					2217:2222	the colons	2213:2222	the colons of mice with DSS-induced colitis	2213:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	23	107	theme	action	3030:3035	arg1	mechanism					3017:3025	The mechanism	3013:3025	The mechanism of action of FCZ and SCZ	3013:3050	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	23	107	theme	action	3030:3035	arg1	related					3062:3068	related	3062:3068	related	3062:3068	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	6	108	theme	SCZ	537:539	arg1	mechanism					524:532	mechanism	524:532	mechanism	524:532	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	6	108	theme	SCZ	537:539	arg1	effect					513:518	effect	513:518	effect	513:518	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	7	109	theme	-induced	723:730	arg1	UC					732:733	dextran sulfate sodium (DSS)-induced UC	695:733	dextran sulfate sodium (DSS)-induced UC in mice	695:741	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	16	110	theme	weight	2088:2093	arg1	loss					2095:2098	weight loss	2088:2098	weight loss	2088:2098	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	111	from	loss	2165:2168	arg1	colons					2217:2222	the colons	2213:2222	the colons of mice with DSS-induced colitis	2213:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	14	112	used	used	1854:1857	arg2	reaction					1841:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction was used to detect the genes of different metabolite-related enzymes.
35114907	4	113	dep	people	289:294	arg1	SCZ					315:317	SCZ	315:317	SCZ	315:317	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	4	113	dep	people	289:294	arg1	lancea					307:312	fry raw A. lancea	296:312	people fry raw A. lancea (SCZ) together with wheat bran	289:343	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	17	114	theme	factors	2359:2365	arg1	activation					2372:2381	activation	2372:2381	activation of pro-inflammatory macrophages in colonic tissue	2372:2431	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	114	theme	factors	2359:2365	arg1	occludin					2318:2325	occludin	2318:2325	occludin	2318:2325	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	114	theme	factors	2359:2365	arg1	production					2328:2337	production	2328:2337	production of pro-inflammatory factors	2328:2365	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	114	theme	factors	2359:2365	arg1	ZO-1					2312:2315	ZO-1	2312:2315	ZO-1	2312:2315	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	17	114	theme	factors	2359:2365	arg1	MUC2					2306:2309	MUC2	2306:2309	MUC2	2306:2309	Moreover, SCZ and FCZ inhibited the decrease in MUC2, ZO-1, occludin, production of pro-inflammatory factors, and activation of pro-inflammatory macrophages in colonic tissue.
35114907	9	115	from	effects	972:978	arg1	loss					990:993	weight loss	983:993	weight loss	983:993	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	115	from	effects	972:978	arg1	changes					1092:1098	pathological changes	1079:1098	pathological changes using the colons from a mouse model of DSS-induced UC	1079:1152	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	115	from	effects	972:978	arg1	loss					1069:1072	goblet cell loss	1057:1072	goblet cell loss	1057:1072	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	115	from	effects	972:978	arg1	index					1013:1017	disease activity index	996:1017	disease activity index	996:1017	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	115	from	effects	972:978	arg1	DAI					1020:1022	DAI	1020:1022	DAI	1020:1022	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	115	from	effects	972:978	arg1	shortening					1045:1054	colon length shortening	1032:1054	colon length shortening	1032:1054	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	20	116	theme	enzymes	2828:2834	arg1	expression					2747:2756	the gene expression	2738:2756	the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes	2738:2834	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	9	117	theme	SCZ	927:929	arg1	effects					916:922	The effects	912:922	The effects of SCZ and FCZ	912:937	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	118	theme	mouse	1124:1128	arg1	model					1130:1134	a mouse model	1122:1134	a mouse model of DSS-induced UC	1122:1152	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	10	119	from	effects	1159:1165	arg1	levels					1185:1190	levels	1185:1190	levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages	1185:1399	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	9	120	theme	FCZ	935:937	arg1	effects					916:922	The effects	912:922	The effects of SCZ and FCZ	912:937	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	21	121	located	observed	2888:2895	arg2	effects					2871:2877	the above effects	2861:2877	the above effects	2861:2877	In the PGF mouse model, the above effects were not observed.
35114907	21	121	located	observed	2888:2895	arg1	model					2854:2858	the PGF mouse model	2840:2858	the PGF mouse model	2840:2858	In the PGF mouse model, the above effects were not observed.
35114907	16	122	from	score	2121:2125	arg1	colons					2217:2222	the colons	2213:2222	the colons of mice with DSS-induced colitis	2213:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	19	123	theme	amino	2671:2675	arg1	acid					2677:2680	amino acid	2671:2680	amino acid	2671:2680	SCZ and FCZ not only inhibited the abundance of harmful bacteria and increased the abundance of beneficial bacteria, but also regulated the metabolism of disease-related metabolites such as amino acid and cholesterol metabolism.
35114907	6	124	theme	ulcerative	552:561	arg1	colitis					563:569	ulcerative colitis	552:569	ulcerative colitis (UC)	552:574	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	6	124	theme	ulcerative	552:561	arg1	UC					572:573	UC	572:573	UC	572:573	Nevertheless, the effect and mechanism of SCZ and FCZ on ulcerative colitis (UC) are still unclear.
35114907	9	125	theme	pathological	1079:1090	arg1	changes					1092:1098	pathological changes	1079:1098	pathological changes using the colons from a mouse model of DSS-induced UC	1079:1152	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	126	theme	UC	1151:1152	arg1	model					1130:1134	a mouse model	1122:1134	a mouse model of DSS-induced UC	1122:1152	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	14	127	theme	transcription-polymerase	1810:1833	arg1	reaction					1841:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction was used to detect the genes of different metabolite-related enzymes.
35114907	20	128	theme	different	2799:2807	arg1	enzymes					2828:2834	different metabolite-related enzymes	2799:2834	different metabolite-related enzymes	2799:2834	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	4	129	theme	raw	300:302	arg1	SCZ					315:317	SCZ	315:317	SCZ	315:317	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	4	129	theme	raw	300:302	arg1	lancea					307:312	fry raw A. lancea	296:312	people fry raw A. lancea (SCZ) together with wheat bran	289:343	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	11	130	theme	intestinal	1551:1560	arg1	flora					1562:1566	the intestinal flora	1547:1566	the intestinal flora	1547:1566	16s RNA sequencing technology was used to detect the composition of the intestinal flora in each group.
35114907	14	131	theme	metabolite-related	1892:1909	arg1	enzymes					1911:1917	different metabolite-related enzymes	1882:1917	different metabolite-related enzymes	1882:1917	Reverse transcription-polymerase chain reaction was used to detect the genes of different metabolite-related enzymes.
35114907	0	132	theme	lancea	13:18	arg1	Rhizoma					20:26	Atractylodes lancea Rhizoma	0:26	Atractylodes lancea Rhizoma	0:26	Atractylodes lancea Rhizoma Attenuates DSS-Induced Colitis by Regulating Intestinal Flora and Metabolites.
35114907	9	133	dep	index	1013:1017	arg1	score					1025:1029	score	1025:1029	score	1025:1029	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	13	134	used	used	1699:1702	arg2	analysis					1686:1693	Pearson analysis	1678:1693	Pearson analysis	1678:1693	Pearson analysis was used to determine the correlation between the intestinal flora, metabolites, and pathological indices.
35114907	9	135	theme	weight	983:988	arg1	loss					990:993	weight loss	983:993	weight loss	983:993	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	16	136	from	extent	2179:2184	arg1	colons					2217:2222	the colons	2213:2222	the colons of mice with DSS-induced colitis	2213:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	15	137	theme	intestinal	2044:2053	arg1	flora					2055:2059	intestinal flora	2044:2059	intestinal flora	2044:2059	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	15	138	from	effect	1987:1992	arg1	UC					2012:2013	UC	2012:2013	UC	2012:2013	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	8	139	theme	SCZ	773:775	arg1	constituents					757:768	The chemical constituents	744:768	The chemical constituents of SCZ and FCZ	744:783	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	4	140	theme	fry	296:298	arg1	SCZ					315:317	SCZ	315:317	SCZ	315:317	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	4	140	theme	fry	296:298	arg1	lancea					307:312	fry raw A. lancea	296:312	people fry raw A. lancea (SCZ) together with wheat bran	289:343	In China, people fry raw A. lancea (SCZ) together with wheat bran to make bran-fried A. lancea (FCZ).
35114907	11	141	theme	RNA	1483:1485	arg1	technology					1498:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology	1479:1507	16s RNA sequencing technology was used to detect the composition of the intestinal flora in each group.
35114907	21	142	theme	PGF	2844:2846	arg1	model					2854:2858	the PGF mouse model	2840:2858	the PGF mouse model	2840:2858	In the PGF mouse model, the above effects were not observed.
35114907	16	143	from	colons	2217:2222	arg1	score					2121:2125	the DAI score	2113:2125	the DAI score	2113:2125	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	143	from	colons	2217:2222	arg1	shortening					2141:2150	colon length shortening	2128:2150	colon length shortening	2128:2150	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	143	from	colons	2217:2222	arg1	extent					2179:2184	the extent	2175:2184	the extent of pathological changes in the colons of mice with DSS-induced colitis	2175:2255	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	16	143	from	colons	2217:2222	arg1	loss					2165:2168	goblet cell loss	2153:2168	goblet cell loss	2153:2168	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	15	144	theme	pseudogerm	1922:1931	arg1	model					1950:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model	1920:1954	A pseudogerm free (PGF) mouse model was used to verify whether the effect of SCZ and FCZ in UC depends on the regulation of intestinal flora.
35114907	8	145	theme	FCZ	781:783	arg1	constituents					757:768	The chemical constituents	744:768	The chemical constituents of SCZ and FCZ	744:783	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	8	146	with	spectrometry	856:867	arg1	compounds					901:909	six reference compounds	887:909	six reference compounds	887:909	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	9	147	theme	activity	1004:1011	arg1	DAI					1020:1022	DAI	1020:1022	DAI	1020:1022	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	147	theme	activity	1004:1011	arg1	index					1013:1017	disease activity index	996:1017	disease activity index	996:1017	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	9	147	theme	activity	1004:1011	arg1	shortening					1045:1054	colon length shortening	1032:1054	colon length shortening	1032:1054	The effects of SCZ and FCZ were investigated based on their effects on weight loss, disease activity index (DAI) score, colon length shortening, goblet cell loss, and pathological changes using the colons from a mouse model of DSS-induced UC.
35114907	7	148	theme	mechanisms	681:690	arg1	effects					648:654	the therapeutic effects	632:654	the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice	632:741	The aim of this study was to compare the therapeutic effects of SCZ and FCZ and their mechanisms on dextran sulfate sodium (DSS)-induced UC in mice.
35114907	23	149	theme	associated	3118:3127	arg1	metabolites					3129:3139	their associated metabolites	3112:3139	their associated metabolites	3112:3139	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	20	150	theme	gene	2742:2745	arg1	expression					2747:2756	the gene expression	2738:2756	the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes	2738:2834	Both preparations inhibited the gene expression (Slc6A7, PRODH, Sdsl, HMGCR, SREBP-2) of different metabolite-related enzymes.
35114907	16	151	theme	goblet	2153:2158	arg1	loss					2165:2168	goblet cell loss	2153:2168	goblet cell loss	2153:2168	SCZ and FCZ could inhibit weight loss and decrease the DAI score, colon length shortening, goblet cell loss, and the extent of pathological changes in the colons of mice with DSS-induced colitis.
35114907	12	152	used	used	1612:1615	arg2	metabonomics					1595:1606	Nontargeted metabonomics	1583:1606	Nontargeted metabonomics	1583:1606	Nontargeted metabonomics was used to detect the serum metabolite levels of mice in each group.
35114907	12	153	theme	metabolite	1637:1646	arg1	levels					1648:1653	the serum metabolite levels	1627:1653	the serum metabolite levels of mice in each group	1627:1675	Nontargeted metabonomics was used to detect the serum metabolite levels of mice in each group.
35114907	8	154	theme	high-performance	805:820	arg1	HPLC-MS/MS					870:879	HPLC-MS/MS	870:879	HPLC-MS/MS	870:879	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	8	154	theme	high-performance	805:820	arg1	spectrometry					856:867	high-performance liquid chromatography-tandem mass spectrometry	805:867	high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds	805:909	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	14	155	theme	chain	1835:1839	arg1	reaction					1841:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction	1802:1848	Reverse transcription-polymerase chain reaction was used to detect the genes of different metabolite-related enzymes.
35114907	10	156	theme	inflammatory	1199:1210	arg1	interleukin-6					1266:1278	interleukin-6	1266:1278	interleukin-6, interleukin-1[Formula: see text]	1266:1312	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	156	theme	inflammatory	1199:1210	arg1	mucoprotein					1315:1325	mucoprotein	1315:1325	mucoprotein (MUC2)	1315:1332	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	156	theme	inflammatory	1199:1210	arg1	cytokines					1212:1220	the inflammatory cytokines	1195:1220	the inflammatory cytokines (tumor necrosis factor-[Formula: see text]	1195:1263	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	156	theme	inflammatory	1199:1210	arg1	protein					1341:1347	tight protein	1335:1347	tight protein (ZO-1, occludin)	1335:1364	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	10	156	theme	inflammatory	1199:1210	arg1	activation					1375:1384	the activation	1371:1384	the activation of macrophages	1371:1399	The effects of SCZ and FCZ on levels of the inflammatory cytokines (tumor necrosis factor-[Formula: see text], interleukin-6, interleukin-1[Formula: see text], mucoprotein (MUC2), tight protein (ZO-1, occludin), and the activation of macrophages were determined using immunohistochemistry (IHC) and immunofluorescence (IF).
35114907	23	157	theme	intestinal	3091:3100	arg1	flora					3102:3106	intestinal flora	3091:3106	intestinal flora	3091:3106	The mechanism of action of FCZ and SCZ is mainly related to the regulation of intestinal flora and their associated metabolites.
35114907	8	158	theme	chromatography-tandem	829:849	arg1	HPLC-MS/MS					870:879	HPLC-MS/MS	870:879	HPLC-MS/MS	870:879	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
35114907	8	158	theme	chromatography-tandem	829:849	arg1	spectrometry					856:867	high-performance liquid chromatography-tandem mass spectrometry	805:867	high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds	805:909	The chemical constituents of SCZ and FCZ were analyzed using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) with six reference compounds.
37371534	4	0	from	therapy	846:852	arg1	patients					862:869	1315 patients	857:869	1315 patients	857:869	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	1	theme	anti-TNF	837:844	arg1	therapy					846:852	anti-TNF therapy	837:852	anti-TNF therapy in 1315 patients	837:869	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	2	theme	N-glycome	678:686	arg1	composition					688:698	N-glycome composition	678:698	N-glycome composition of total serum IgG in 198 patients	678:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	3	theme	UHPLC	557:561	arg1	method					563:568	the high-throughput UHPLC method	537:568	the high-throughput UHPLC method for N-glycan analysis	537:590	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	5	4	theme	diminished	1107:1116	arg1	environment					1131:1141	a diminished inflammatory environment	1105:1141	a diminished inflammatory environment in CD patients treated with anti-TNF therapy	1105:1186	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	6	5	theme	anti-TNF	1281:1288	arg1	outcome					1298:1304	the anti-TNF therapy outcome	1277:1304	the anti-TNF therapy outcome	1277:1304	Furthermore, we observed that IgG N-glycome might contain certain information regarding the anti-TNF therapy outcome before initiating the treatment.
37371534	3	6	theme	N-glycome	476:484	arg1	patients					443:450	CD patients	440:450	CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals	440:516	Previous studies have demonstrated a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals.
37371534	1	7	theme	well-being	226:235	arg1	quality					206:212	patients' quality	196:212	patients' quality of life and well-being	196:235	Crohn's disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being.
37371534	5	8	theme	inflammatory	1118:1129	arg1	environment					1131:1141	a diminished inflammatory environment	1105:1141	a diminished inflammatory environment in CD patients treated with anti-TNF therapy	1105:1186	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	9	from	environment	1131:1141	arg1	patients					1149:1156	CD patients	1146:1156	CD patients treated with anti-TNF therapy	1146:1186	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	7	10	theme	IgG	1442:1444	arg1	composition					1456:1466	IgG N-glycome composition	1442:1466	IgG N-glycome composition at baseline	1442:1478	However, it is impossible to predict future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline.
37371534	5	11	from	increase	940:947	arg1	agalactosylation					916:931	IgG agalactosylation	912:931	IgG agalactosylation	912:931	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	11	from	increase	940:947	arg1	sialylation					995:1005	sialylation	995:1005	sialylation	995:1005	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	11	from	increase	940:947	arg1	digalactosylation					973:989	digalactosylation	973:989	digalactosylation	973:989	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	11	from	increase	940:947	arg1	monogalactosylation					952:970	monogalactosylation	952:970	monogalactosylation	952:970	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	7	12	theme	N-glycome	1446:1454	arg1	composition					1456:1466	IgG N-glycome composition	1442:1466	IgG N-glycome composition at baseline	1442:1478	However, it is impossible to predict future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline.
37371534	1	13	theme	chronic	125:131	arg1	disease					107:113	Crohn's disease	99:113	Crohn's disease (CD)	99:118	Crohn's disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being.
37371534	1	13	theme	chronic	125:131	arg1	inflammation					133:144	a chronic inflammation	123:144	a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being	123:235	Crohn's disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being.
37371534	4	14	theme	total	703:707	arg1	IgG					715:717	total serum IgG	703:717	total serum IgG in 198 patients	703:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	3	15	theme	healthy	498:504	arg1	individuals					506:516	healthy individuals	498:516	healthy individuals	498:516	Previous studies have demonstrated a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals.
37371534	5	16	theme	significant	888:898	arg1	decrease					900:907	a significant decrease	886:907	a significant decrease in IgG agalactosylation	886:931	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	4	17	theme	anti-TNF	634:641	arg1	drugs					643:647	the anti-TNF drugs	630:647	the anti-TNF drugs infliximab and adalimumab	630:673	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	17	theme	anti-TNF	634:641	arg1	adalimumab					664:673	adalimumab	664:673	adalimumab	664:673	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	17	theme	anti-TNF	634:641	arg1	infliximab					649:658	infliximab	649:658	infliximab	649:658	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	0	18	from	Properties	51:60	arg1	Disease					90:96	Crohn's Disease	82:96	Crohn's Disease	82:96	Anti-TNF Biologicals Enhance the Anti-Inflammatory Properties of IgG N-Glycome in Crohn's Disease.
37371534	6	19	theme	certain	1247:1253	arg1	information					1255:1265	certain information	1247:1265	certain information regarding the anti-TNF therapy outcome	1247:1304	Furthermore, we observed that IgG N-glycome might contain certain information regarding the anti-TNF therapy outcome before initiating the treatment.
37371534	4	20	from	patients	726:733	arg1	composition					688:698	N-glycome composition	678:698	N-glycome composition of total serum IgG in 198 patients	678:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	3	21	theme	Previous	356:363	arg1	studies					365:371	Previous studies	356:371	Previous studies	356:371	Previous studies have demonstrated a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals.
37371534	6	22	theme	therapy	1290:1296	arg1	outcome					1298:1304	the anti-TNF therapy outcome	1277:1304	the anti-TNF therapy outcome	1277:1304	Furthermore, we observed that IgG N-glycome might contain certain information regarding the anti-TNF therapy outcome before initiating the treatment.
37371534	5	23	from	decrease	900:907	arg1	agalactosylation					916:931	IgG agalactosylation	912:931	IgG agalactosylation	912:931	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	23	from	decrease	900:907	arg1	sialylation					995:1005	sialylation	995:1005	sialylation	995:1005	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	23	from	decrease	900:907	arg1	digalactosylation					973:989	digalactosylation	973:989	digalactosylation	973:989	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	23	from	decrease	900:907	arg1	monogalactosylation					952:970	monogalactosylation	952:970	monogalactosylation	952:970	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	1	24	theme	digestive	153:161	arg1	tract					163:167	the digestive tract	149:167	the digestive tract	149:167	Crohn's disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being.
37371534	0	25	theme	Anti-TNF	0:7	arg1	Biologicals					9:19	Anti-TNF Biologicals	0:19	Anti-TNF Biologicals	0:19	Anti-TNF Biologicals Enhance the Anti-Inflammatory Properties of IgG N-Glycome in Crohn's Disease.
37371534	4	26	theme	primary	811:817	arg1	non-responders					819:832	primary non-responders	811:832	primary non-responders to anti-TNF therapy in 1315 patients	811:869	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	27	theme	IgG	715:717	arg1	composition					688:698	N-glycome composition	678:698	N-glycome composition of total serum IgG in 198 patients	678:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	1	28	theme	tract	163:167	arg1	disease					107:113	Crohn's disease	99:113	Crohn's disease (CD)	99:118	Crohn's disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being.
37371534	1	28	theme	tract	163:167	arg1	inflammation					133:144	a chronic inflammation	123:144	a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being	123:235	Crohn's disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being.
37371534	7	29	from	baseline	1471:1478	arg1	composition					1456:1466	IgG N-glycome composition	1442:1466	IgG N-glycome composition at baseline	1442:1478	However, it is impossible to predict future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline.
37371534	4	30	from	IgG	715:717	arg1	patients					726:733	198 patients	722:733	198 patients	722:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	2	31	theme	CD	291:292	arg1	treatment					278:286	the treatment	274:286	the treatment of CD	274:292	Anti-TNF biologicals revolutionised the treatment of CD, yet many patients do not adequately respond to such therapy.
37371534	4	32	from	effect	620:625	arg1	composition					688:698	N-glycome composition	678:698	N-glycome composition of total serum IgG in 198 patients	678:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	3	33	theme	patients	443:450	arg1	composition					425:435	the composition	421:435	the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals	421:516	Previous studies have demonstrated a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals.
37371534	4	34	from	potential	762:770	arg1	baseline					792:799	baseline	792:799	baseline	792:799	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	5	35	theme	anti-TNF	1171:1178	arg1	therapy					1180:1186	anti-TNF therapy	1171:1186	anti-TNF therapy	1171:1186	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	5	36	theme	IgG	912:914	arg1	agalactosylation					916:931	IgG agalactosylation	912:931	IgG agalactosylation	912:931	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	7	37	theme	anti-TNF	1409:1416	arg1	therapy					1418:1424	anti-TNF therapy	1409:1424	anti-TNF therapy	1409:1424	However, it is impossible to predict future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline.
37371534	4	38	theme	high-throughput	541:555	arg1	method					563:568	the high-throughput UHPLC method	537:568	the high-throughput UHPLC method for N-glycan analysis	537:590	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	39	theme	N-glycans	779:787	arg1	potential					762:770	the predictive potential	747:770	the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients	747:869	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	40	theme	drugs	643:647	arg1	effect					620:625	the longitudinal effect	603:625	the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients	603:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	3	41	theme	pro-inflammatory	393:408	arg1	pattern					410:416	a pro-inflammatory pattern	391:416	a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals	391:516	Previous studies have demonstrated a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals.
37371534	0	42	theme	Anti-Inflammatory	33:49	arg1	Properties					51:60	the Anti-Inflammatory Properties	29:60	the Anti-Inflammatory Properties of IgG N-Glycome in Crohn's Disease	29:96	Anti-TNF Biologicals Enhance the Anti-Inflammatory Properties of IgG N-Glycome in Crohn's Disease.
37371534	5	43	theme	therapy	1064:1070	arg1	response					1072:1079	therapy response	1064:1079	therapy response	1064:1079	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	4	44	theme	IgG	775:777	arg1	N-glycans					779:787	IgG N-glycans	775:787	IgG N-glycans at baseline	775:799	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	45	from	baseline	792:799	arg1	potential					762:770	the predictive potential	747:770	the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients	747:869	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	45	from	baseline	792:799	arg1	N-glycans					779:787	IgG N-glycans	775:787	IgG N-glycans at baseline	775:799	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	3	46	theme	CD	440:441	arg1	patients					443:450	CD patients	440:450	CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals	440:516	Previous studies have demonstrated a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals.
37371534	0	47	theme	IgG	65:67	arg1	N-Glycome					69:77	IgG N-Glycome	65:77	IgG N-Glycome	65:77	Anti-TNF Biologicals Enhance the Anti-Inflammatory Properties of IgG N-Glycome in Crohn's Disease.
37371534	6	48	contain	contain	1239:1245	arg2	information					1255:1265	certain information	1247:1265	certain information regarding the anti-TNF therapy outcome	1247:1304	Furthermore, we observed that IgG N-glycome might contain certain information regarding the anti-TNF therapy outcome before initiating the treatment.
37371534	6	48	contain	contain	1239:1245	arg1	N-glycome					1223:1231	IgG N-glycome	1219:1231	IgG N-glycome	1219:1231	Furthermore, we observed that IgG N-glycome might contain certain information regarding the anti-TNF therapy outcome before initiating the treatment.
37371534	5	49	theme	CD	1146:1147	arg1	patients					1149:1156	CD patients	1146:1156	CD patients treated with anti-TNF therapy	1146:1186	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	7	50	theme	primary	1383:1389	arg1	non-responders					1391:1404	future primary non-responders	1376:1404	future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline	1376:1478	However, it is impossible to predict future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline.
37371534	5	51	theme	anti-TNF	1030:1037	arg1	treatment					1039:1047	anti-TNF treatment	1030:1047	anti-TNF treatment	1030:1047	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	4	52	theme	predictive	751:760	arg1	potential					762:770	the predictive potential	747:770	the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients	747:869	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	2	53	theme	Anti-TNF	238:245	arg1	biologicals					247:257	Anti-TNF biologicals	238:257	Anti-TNF biologicals	238:257	Anti-TNF biologicals revolutionised the treatment of CD, yet many patients do not adequately respond to such therapy.
37371534	6	54	theme	IgG	1219:1221	arg1	N-glycome					1223:1231	IgG N-glycome	1219:1231	IgG N-glycome	1219:1231	Furthermore, we observed that IgG N-glycome might contain certain information regarding the anti-TNF therapy outcome before initiating the treatment.
37371534	4	55	theme	serum	709:713	arg1	IgG					715:717	total serum IgG	703:717	total serum IgG in 198 patients	703:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	5	56	theme	treatment	1039:1047	arg1	weeks					1021:1025	the 14 weeks	1014:1025	the 14 weeks of anti-TNF treatment	1014:1047	We discovered a significant decrease in IgG agalactosylation and an increase in monogalactosylation, digalactosylation and sialylation during the 14 weeks of anti-TNF treatment, regardless of therapy response, all of which suggested a diminished inflammatory environment in CD patients treated with anti-TNF therapy.
37371534	4	57	dep	drugs	643:647	arg1	drugs					643:647	the anti-TNF drugs	630:647	the anti-TNF drugs infliximab and adalimumab	630:673	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	57	dep	drugs	643:647	arg1	adalimumab					664:673	adalimumab	664:673	adalimumab	664:673	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	57	dep	drugs	643:647	arg1	infliximab					649:658	infliximab	649:658	infliximab	649:658	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	3	58	from	pattern	410:416	arg1	composition					425:435	the composition	421:435	the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals	421:516	Previous studies have demonstrated a pro-inflammatory pattern in the composition of CD patients' immunoglobulin G (IgG) N-glycome compared to healthy individuals.
37371534	2	59	theme	many	299:302	arg1	patients					304:311	many patients	299:311	many patients	299:311	Anti-TNF biologicals revolutionised the treatment of CD, yet many patients do not adequately respond to such therapy.
37371534	4	60	theme	N-glycan	574:581	arg1	analysis					583:590	N-glycan analysis	574:590	N-glycan analysis	574:590	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	4	61	theme	longitudinal	607:618	arg1	effect					620:625	the longitudinal effect	603:625	the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients	603:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	2	62	theme	such	342:345	arg1	therapy					347:353	such therapy	342:353	such therapy	342:353	Anti-TNF biologicals revolutionised the treatment of CD, yet many patients do not adequately respond to such therapy.
37371534	4	63	from	composition	688:698	arg1	patients					726:733	198 patients	722:733	198 patients	722:733	Here, we utilised the high-throughput UHPLC method for N-glycan analysis to explore the longitudinal effect of the anti-TNF drugs infliximab and adalimumab on N-glycome composition of total serum IgG in 198 patients, as well as the predictive potential of IgG N-glycans at baseline to detect primary non-responders to anti-TNF therapy in 1315 patients.
37371534	0	64	theme	N-Glycome	69:77	arg1	Properties					51:60	the Anti-Inflammatory Properties	29:60	the Anti-Inflammatory Properties of IgG N-Glycome in Crohn's Disease	29:96	Anti-TNF Biologicals Enhance the Anti-Inflammatory Properties of IgG N-Glycome in Crohn's Disease.
37371534	7	65	theme	future	1376:1381	arg1	non-responders					1391:1404	future primary non-responders	1376:1404	future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline	1376:1478	However, it is impossible to predict future primary non-responders to anti-TNF therapy based solely on IgG N-glycome composition at baseline.
37371534	1	66	theme	life	217:220	arg1	quality					206:212	patients' quality	196:212	patients' quality of life and well-being	196:235	Crohn's disease (CD) is a chronic inflammation of the digestive tract that significantly impairs patients' quality of life and well-being.
35894777	0	0	theme	human	104:108	arg1	RBCs					110:113	human RBCs	104:113	human RBCs	104:113	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	2	1	theme	human	511:515	arg1	hRBCs					534:538	hRBCs	534:538	hRBCs	534:538	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	2	1	theme	human	511:515	arg1	cells					527:531	human red blood cells	511:531	human red blood cells (hRBCs)	511:539	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	4	2	contain	containing	1017:1026	arg2	γ-PGA					1053:1057	phenylbutylamine-grafted γ-PGA	1028:1057	phenylbutylamine-grafted γ-PGA	1028:1057	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	4	2	contain	containing	1017:1026	arg1	trehalose					1007:1015	0.8 M trehalose	1001:1015	0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival	1001:1281	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	6	3	theme	biocompatible	1563:1575	arg1	cryoprotectant					1577:1590	the biocompatible cryoprotectant	1559:1590	the biocompatible cryoprotectant	1559:1590	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	6	3	theme	biocompatible	1563:1575	arg1	trehalose					1546:1554	trehalose	1546:1554	trehalose	1546:1554	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	4	4	theme	phenylbutylamine-grafted	1028:1051	arg1	γ-PGA					1053:1057	phenylbutylamine-grafted γ-PGA	1028:1057	phenylbutylamine-grafted γ-PGA	1028:1057	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	4	5	theme	spacer	921:926	arg1	length					928:933	the methylene spacer length	907:933	the methylene spacer length	907:933	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	3	6	theme	cell	660:663	arg1	membrane					665:672	the cell membrane	656:672	the cell membrane	656:672	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	5	7	theme	Molecular	1284:1292	arg1	dynamics					1294:1301	Molecular dynamics	1284:1301	Molecular dynamics simulation	1284:1312	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	5	8	theme	dynamics	1294:1301	arg1	simulation					1303:1312	Molecular dynamics simulation	1284:1312	Molecular dynamics simulation	1284:1312	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	0	9	theme	RBCs	110:113	arg1	cryopreservation					84:99	cryopreservation	84:99	cryopreservation of human RBCs with high intracellular trehalose	84:147	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	0	10	with	cryopreservation	84:99	arg1	trehalose					139:147	high intracellular trehalose	120:147	high intracellular trehalose	120:147	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	5	11	theme	polymers	1406:1413	arg1	interaction					1364:1374	interaction	1364:1374	interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer	1364:1449	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	0	12	theme	intracellular	125:137	arg1	trehalose					139:147	high intracellular trehalose	120:147	high intracellular trehalose	120:147	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	3	13	theme	membrane	755:762	arg1	permeability					764:775	the membrane permeability	751:775	the membrane permeability of trehalose	751:788	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	5	14	theme	modeled	1422:1428	arg1	bilayer					1443:1449	the modeled phospholipid bilayer	1418:1449	the modeled phospholipid bilayer	1418:1449	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	6	15	theme	glycerol-free	1473:1485	arg1	cryopreservation					1487:1502	glycerol-free cryopreservation	1473:1502	glycerol-free cryopreservation of human cells	1473:1517	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	0	16	theme	high	120:123	arg1	trehalose					139:147	high intracellular trehalose	120:147	high intracellular trehalose	120:147	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	2	17	from	uptake	501:506	arg1	hRBCs					534:538	hRBCs	534:538	hRBCs	534:538	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	2	17	from	uptake	501:506	arg1	cells					527:531	human red blood cells	511:531	human red blood cells (hRBCs)	511:539	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	5	18	theme	phospholipid	1430:1441	arg1	bilayer					1443:1449	the modeled phospholipid bilayer	1418:1449	the modeled phospholipid bilayer	1418:1449	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	4	19	theme	M	1005:1005	arg1	trehalose					1007:1015	0.8 M trehalose	1001:1015	0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival	1001:1281	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	4	20	theme	methylene	911:919	arg1	length					928:933	the methylene spacer length	907:933	the methylene spacer length	907:933	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	6	21	theme	cells	1513:1517	arg1	cryopreservation					1487:1502	glycerol-free cryopreservation	1473:1502	glycerol-free cryopreservation of human cells	1473:1517	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	0	22	theme	Membrane	0:7	arg1	stabilization					9:21	Membrane stabilization	0:21	Membrane stabilization	0:21	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	3	23	theme	trehalose	780:788	arg1	permeability					764:775	the membrane permeability	751:775	the membrane permeability of trehalose	751:788	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	6	24	theme	compensatory	1629:1640	arg1	approach					1642:1649	a compensatory approach	1627:1649	a compensatory approach to membrane perturbation	1627:1674	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	6	24	theme	compensatory	1629:1640	arg1	stabilization					1606:1618	membrane stabilization	1597:1618	membrane stabilization	1597:1618	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	1	25	theme	cryoprotective	320:333	arg1	efficiency					335:344	its cryoprotective efficiency	316:344	its cryoprotective efficiency	316:344	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	5	26	from	interaction	1364:1374	arg1	bilayer					1443:1449	the modeled phospholipid bilayer	1418:1449	the modeled phospholipid bilayer	1418:1449	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	5	27	theme	amphiphilic	1394:1404	arg1	polymers					1406:1413	the above four amphiphilic polymers	1379:1413	the above four amphiphilic polymers	1379:1413	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	4	28	dep	h.	1074:1075	arg1	exhibited					1113:1121	exhibited	1113:1121	exhibited	1113:1121	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	6	29	theme	biomolecule	1695:1705	arg1	delivery					1707:1714	impermeable biomolecule delivery	1683:1714	impermeable biomolecule delivery	1683:1714	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	4	30	theme	0.7	971:973	arg1	±					969:969	±	969:969	±	969:969	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	3	31	theme	self-assembled	572:585	arg1	nanoparticles					587:599	The self-assembled nanoparticles	568:599	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA	568:633	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	4	32	theme	intracellular	1170:1182	arg1	trehalose					1184:1192	improved intracellular trehalose	1161:1192	improved intracellular trehalose	1161:1192	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	1	33	theme	biocompatible	208:220	arg1	cryoprotectant					222:235	a biocompatible cryoprotectant	206:235	a biocompatible cryoprotectant for solvent-free cell cryopreservation	206:274	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	1	33	theme	biocompatible	208:220	arg1	trehalose					181:189	trehalose	181:189	trehalose	181:189	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	0	34	theme	aromatic	46:53	arg1	γ-PGA					74:78	aromatic monoamine-modified γ-PGA	46:78	aromatic monoamine-modified γ-PGA	46:78	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	6	35	theme	membrane	1654:1661	arg1	perturbation					1663:1674	membrane perturbation	1654:1674	membrane perturbation	1654:1674	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	2	36	theme	membrane	545:552	arg1	perturbation					554:565	membrane perturbation	545:565	membrane perturbation	545:565	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	3	37	theme	amphiphilic	617:627	arg1	γ-PGA					629:633	the obtained amphiphilic γ-PGA	604:633	the obtained amphiphilic γ-PGA	604:633	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	4	38	theme	improved	1161:1168	arg1	trehalose					1184:1192	improved intracellular trehalose	1161:1192	improved intracellular trehalose	1161:1192	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	2	39	theme	glutamic	440:447	arg1	γ-poly					433:438	γ-poly	433:438	γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation	433:565	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	2	39	theme	glutamic	440:447	arg1	acid					449:452	glutamic acid	440:452	glutamic acid	440:452	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	2	40	theme	trehalose	491:499	arg1	uptake					501:506	intracellular trehalose uptake	477:506	intracellular trehalose uptake in human red blood cells (hRBCs)	477:539	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	3	41	theme	γ-PGA	629:633	arg1	nanoparticles					587:599	The self-assembled nanoparticles	568:599	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA	568:633	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	4	42	theme	0.8	1001:1003	arg1	M					1005:1005	M	1005:1005	M	1005:1005	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	2	43	theme	intracellular	477:489	arg1	uptake					501:506	intracellular trehalose uptake	477:506	intracellular trehalose uptake in human red blood cells (hRBCs)	477:539	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	2	44	with	monoamines	376:385	arg1	spacer					408:413	a 1-4 methylene spacer	392:413	a 1-4 methylene spacer	392:413	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	0	45	theme	monoamine-modified	55:72	arg1	γ-PGA					74:78	aromatic monoamine-modified γ-PGA	46:78	aromatic monoamine-modified γ-PGA	46:78	Membrane stabilization versus perturbation by aromatic monoamine-modified γ-PGA for cryopreservation of human RBCs with high intracellular trehalose.
35894777	3	46	theme	hypertonic	796:805	arg1	conditions					807:816	hypertonic conditions	796:816	hypertonic conditions	796:816	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	3	47	theme	obtained	608:615	arg1	γ-PGA					629:633	the obtained amphiphilic γ-PGA	604:633	the obtained amphiphilic γ-PGA	604:633	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	2	48	theme	monoamines	376:385	arg1	series					357:362	a series	355:362	a series of aromatic monoamines with a 1-4 methylene spacer	355:413	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	4	49	theme	membrane	1123:1130	arg1	stabilization					1132:1144	membrane stabilization	1123:1144	membrane stabilization	1123:1144	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	4	50	theme	high	1265:1268	arg1	cryosurvival					1270:1281	high cryosurvival	1265:1281	high cryosurvival	1265:1281	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	2	51	theme	aromatic	367:374	arg1	monoamines					376:385	aromatic monoamines	367:385	aromatic monoamines with a 1-4 methylene spacer	367:413	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	6	52	theme	membrane	1597:1604	arg1	approach					1642:1649	a compensatory approach	1627:1649	a compensatory approach to membrane perturbation	1627:1674	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	6	52	theme	membrane	1597:1604	arg1	stabilization					1606:1618	membrane stabilization	1597:1618	membrane stabilization	1597:1618	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	1	53	theme	solvent-free	241:252	arg1	cryopreservation					259:274	solvent-free cell cryopreservation	241:274	solvent-free cell cryopreservation	241:274	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	4	54	theme	75.1	964:967	arg1	±					969:969	±	969:969	±	969:969	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	1	55	used	used	198:201	arg2	trehalose					181:189	trehalose	181:189	trehalose	181:189	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	1	55	used	used	198:201	arg2	disaccharide					167:178	a nonreducing disaccharide	153:178	a nonreducing disaccharide	153:178	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	1	55	used	used	198:201	arg2	cryoprotectant					222:235	a biocompatible cryoprotectant	206:235	a biocompatible cryoprotectant for solvent-free cell cryopreservation	206:274	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	1	56	theme	cell	254:257	arg1	cryopreservation					259:274	solvent-free cell cryopreservation	241:274	solvent-free cell cryopreservation	241:274	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	2	57	theme	blood	521:525	arg1	hRBCs					534:538	hRBCs	534:538	hRBCs	534:538	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	2	57	theme	blood	521:525	arg1	cells					527:531	human red blood cells	511:531	human red blood cells (hRBCs)	511:539	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	3	58	theme	hydrophobic	681:691	arg1	interaction					693:703	the hydrophobic interaction	677:703	the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions	677:816	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	4	59	theme	intracellular	846:858	arg1	trehalose					860:868	the intracellular trehalose	842:868	the intracellular trehalose	842:868	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	4	60	theme	membrane	1211:1218	arg1	integrity					1220:1228	the membrane integrity	1207:1228	the membrane integrity	1207:1228	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	1	61	theme	nonreducing	155:165	arg1	disaccharide					167:178	a nonreducing disaccharide	153:178	a nonreducing disaccharide	153:178	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	1	61	theme	nonreducing	155:165	arg1	trehalose					181:189	trehalose	181:189	trehalose	181:189	As a nonreducing disaccharide, trehalose can be used as a biocompatible cryoprotectant for solvent-free cell cryopreservation, but the membrane-impermeability limits its cryoprotective efficiency.
35894777	5	62	theme	above	1383:1387	arg1	polymers					1406:1413	the above four amphiphilic polymers	1379:1413	the above four amphiphilic polymers	1379:1413	Molecular dynamics simulation further confirmed that defects could be formed by interaction of the above four amphiphilic polymers on the modeled phospholipid bilayer.
35894777	6	63	theme	human	1507:1511	arg1	cells					1513:1517	human cells	1507:1517	human cells	1507:1517	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	2	64	theme	red	517:519	arg1	hRBCs					534:538	hRBCs	534:538	hRBCs	534:538	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	2	64	theme	red	517:519	arg1	cells					527:531	human red blood cells	511:531	human red blood cells (hRBCs)	511:539	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	3	65	theme	lipid	720:724	arg1	arrangement					726:736	the lipid arrangement	716:736	the lipid arrangement	716:736	The self-assembled nanoparticles of the obtained amphiphilic γ-PGA could be adsorbed on the cell membrane by the hydrophobic interaction to disturb the lipid arrangement and increase the membrane permeability of trehalose under hypertonic conditions.
35894777	6	66	theme	impermeable	1683:1693	arg1	delivery					1707:1714	impermeable biomolecule delivery	1683:1714	impermeable biomolecule delivery	1683:1714	It was believed that glycerol-free cryopreservation of human cells could be realized by using trehalose as the biocompatible cryoprotectant, and membrane stabilization can be a compensatory approach to membrane perturbation during impermeable biomolecule delivery.
35894777	2	67	theme	methylene	398:406	arg1	spacer					408:413	a 1-4 methylene spacer	392:413	a 1-4 methylene spacer	392:413	Herein, a series of aromatic monoamines with a 1-4 methylene spacer were grafted onto γ-poly(glutamic acid) (γ-PGA) for promoting intracellular trehalose uptake in human red blood cells (hRBCs) via membrane perturbation.
35894777	4	68	theme	other	1092:1096	arg1	polymers					1104:1111	the other three polymers	1088:1111	the other three polymers	1088:1111	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
35894777	4	68	theme	other	1092:1096	arg1	Meanwhile					1077:1085	Meanwhile	1077:1085	Meanwhile	1077:1085	Results suggested that the intracellular trehalose could be enhanced progressively with the methylene spacer length, significantly increasing to 75.1 ± 0.7 mM by incubating hRBCs in 0.8 M trehalose containing phenylbutylamine-grafted γ-PGA at 4 °C for 24 h. Meanwhile, the other three polymers exhibited membrane stabilization in addition to improved intracellular trehalose, maintaining the membrane integrity during cryopreservation to achieve high cryosurvival.
37403713	7	0	theme	regeneration	1518:1529	arg1	suppression					1536:1546	suppression	1536:1546	suppression of hepatocyte apoptosis	1536:1570	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	0	theme	regeneration	1518:1529	arg1	activation					1498:1507	activation	1498:1507	activation of liver regeneration	1498:1529	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	0	theme	regeneration	1518:1529	arg1	initiation					1468:1477	initiation	1468:1477	initiation of cytokine storm	1468:1495	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	4	1	theme	cytokines	916:924	arg1	levels					889:894	the levels	885:894	the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α)	885:1008	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	7	2	theme	cytokine	1482:1489	arg1	storm					1491:1495	cytokine storm	1482:1495	cytokine storm	1482:1495	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	5	3	theme	proteomics	1070:1079	arg1	analyses					1081:1088	DIA proteomics analyses	1066:1088	DIA proteomics analyses	1066:1088	Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments.
37403713	7	4	theme	storm	1491:1495	arg1	suppression					1536:1546	suppression	1536:1546	suppression of hepatocyte apoptosis	1536:1570	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	4	theme	storm	1491:1495	arg1	activation					1498:1507	activation	1498:1507	activation of liver regeneration	1498:1529	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	4	theme	storm	1491:1495	arg1	initiation					1468:1477	initiation	1468:1477	initiation of cytokine storm	1468:1495	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	1	5	theme	phenolic-enriched	298:314	arg1	MSX					337:339	MSX	337:339	MSX	337:339	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	1	5	theme	phenolic-enriched	298:314	arg1	extract					328:334	a phenolic-enriched maple syrup extract	296:334	a phenolic-enriched maple syrup extract (MSX)	296:340	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	0	6	theme	lipopolysaccharide-induced	88:113	arg1	peritonitis					115:125	lipopolysaccharide-induced peritonitis	88:125	lipopolysaccharide-induced peritonitis in mice	88:133	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	1	7	theme	several	395:401	arg1	diabetes					428:435	diabetes	428:435	diabetes	428:435	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	1	7	theme	several	395:401	arg1	models					411:416	several disease models	395:416	several disease models including diabetes and Alzheimer's disease	395:459	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	4	8	theme	pro-inflammatory	899:914	arg1	cytokines					916:924	pro-inflammatory cytokines	899:924	pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α)	899:1008	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	8	theme	pro-inflammatory	899:914	arg1	beta					950:953	interleukin-1 beta	936:953	interleukin-1 beta (IL-1β)	936:961	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	8	theme	pro-inflammatory	899:914	arg1	alpha					996:1000	tumor necrosis factor alpha	974:1000	tumor necrosis factor alpha (TNF-α)	974:1008	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	8	theme	pro-inflammatory	899:914	arg1	IL-6					964:967	IL-6	964:967	IL-6	964:967	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	7	9	theme	apoptosis	1562:1570	arg1	suppression					1536:1546	suppression	1536:1546	suppression of hepatocyte apoptosis	1536:1570	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	9	theme	apoptosis	1562:1570	arg1	activation					1498:1507	activation	1498:1507	activation of liver regeneration	1498:1529	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	9	theme	apoptosis	1562:1570	arg1	initiation					1468:1477	initiation	1468:1477	initiation of cytokine storm	1468:1495	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	8	10	theme	critical	1743:1750	arg1	insight					1752:1758	critical insight	1743:1758	critical insight to its therapeutic potential	1743:1787	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	1	11	theme	maple	316:320	arg1	MSX					337:339	MSX	337:339	MSX	337:339	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	1	11	theme	maple	316:320	arg1	extract					328:334	a phenolic-enriched maple syrup extract	296:334	a phenolic-enriched maple syrup extract (MSX)	296:340	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	4	12	theme	necrosis	980:987	arg1	TNF-α					1003:1007	TNF-α	1003:1007	TNF-α	1003:1007	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	12	theme	necrosis	980:987	arg1	alpha					996:1000	tumor necrosis factor alpha	974:1000	tumor necrosis factor alpha (TNF-α)	974:1008	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	1	13	theme	disease	403:409	arg1	diabetes					428:435	diabetes	428:435	diabetes	428:435	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	1	13	theme	disease	403:409	arg1	models					411:416	several disease models	395:416	several disease models including diabetes and Alzheimer's disease	395:459	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	4	14	theme	interleukin-1	936:948	arg1	IL-1β					956:960	IL-1β	956:960	IL-1β	956:960	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	14	theme	interleukin-1	936:948	arg1	beta					950:953	interleukin-1 beta	936:953	interleukin-1 beta (IL-1β)	936:961	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	1	15	theme	syrup	322:326	arg1	MSX					337:339	MSX	337:339	MSX	337:339	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	1	15	theme	syrup	322:326	arg1	extract					328:334	a phenolic-enriched maple syrup extract	296:334	a phenolic-enriched maple syrup extract (MSX)	296:340	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	4	16	theme	tumor	974:978	arg1	TNF-α					1003:1007	TNF-α	1003:1007	TNF-α	1003:1007	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	16	theme	tumor	974:978	arg1	alpha					996:1000	tumor necrosis factor alpha	974:1000	tumor necrosis factor alpha (TNF-α)	974:1008	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	3	17	theme	proteomics	774:783	arg1	assay					785:789	data-independent acquisition (DIA) proteomics assay	739:789	data-independent acquisition (DIA) proteomics assay	739:789	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	7	18	theme	initiation	1468:1477	arg1	processes					1455:1463	the processes	1451:1463	the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis	1451:1570	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	19	theme	liver	1512:1516	arg1	regeneration					1518:1529	liver regeneration	1512:1529	liver regeneration	1512:1529	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	0	20	from	analysis	190:197	arg1	insights					136:143	insights	136:143	insights from data-independent acquisition proteomics analysis	136:197	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	1	21	theme	extract	328:334	arg1	composition					255:265	phytochemical composition	241:265	phytochemical composition	241:265	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	1	21	theme	extract	328:334	arg1	activities					282:291	biological activities	271:291	biological activities	271:291	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	3	22	theme	DIA	769:771	arg1	assay					785:789	data-independent acquisition (DIA) proteomics assay	739:789	data-independent acquisition (DIA) proteomics assay	739:789	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	6	23	theme	MSX	1256:1258	arg1	treatment					1260:1268	MSX treatment	1256:1268	MSX treatment	1256:1268	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	8	24	theme	therapeutic	1767:1777	arg1	potential					1779:1787	its therapeutic potential	1763:1787	its therapeutic potential	1763:1787	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	6	25	theme	inflammatory	1293:1304	arg1	TNF					1357:1359	TNF	1357:1359	TNF	1357:1359	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	6	25	theme	inflammatory	1293:1304	arg1	regulators					1315:1324	several inflammatory upstream regulators	1285:1324	several inflammatory upstream regulators including interferon gamma and TNF	1285:1359	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	6	25	theme	inflammatory	1293:1304	arg1	gamma					1347:1351	interferon gamma	1336:1351	interferon gamma	1336:1351	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	3	26	theme	MSX	618:620	arg1	efficacy					606:613	the efficacy	602:613	the efficacy of MSX in a peritonitis mouse model	602:649	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	3	27	theme	data-independent	739:754	arg1	assay					785:789	data-independent acquisition (DIA) proteomics assay	739:789	data-independent acquisition (DIA) proteomics assay	739:789	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	2	28	theme	MSX	496:498	arg1	doses					487:491	the efficacious doses	471:491	the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects	471:566	However, the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects are not fully elucidated.
37403713	5	29	dep	altered	1145:1151	arg1	down-regulated					1167:1180	down-regulated	1167:1180	down-regulated	1167:1180	Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments.
37403713	5	29	dep	altered	1145:1151	arg1	up-					1159:1161	up-	1159:1161	up-	1159:1161	Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments.
37403713	4	30	theme	major	1027:1031	arg1	organs					1033:1038	major organs	1027:1038	major organs	1027:1038	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	6	31	theme	several	1285:1291	arg1	TNF					1357:1359	TNF	1357:1359	TNF	1357:1359	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	6	31	theme	several	1285:1291	arg1	regulators					1315:1324	several inflammatory upstream regulators	1285:1324	several inflammatory upstream regulators including interferon gamma and TNF	1285:1359	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	6	31	theme	several	1285:1291	arg1	gamma					1347:1351	interferon gamma	1336:1351	interferon gamma	1336:1351	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	4	32	theme	mice	1047:1050	arg1	serum					1017:1021	serum	1017:1021	serum	1017:1021	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	32	theme	mice	1047:1050	arg1	organs					1033:1038	major organs	1027:1038	major organs	1027:1038	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	0	33	theme	data-independent	150:165	arg1	analysis					190:197	data-independent acquisition proteomics analysis	150:197	data-independent acquisition proteomics analysis	150:197	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	7	34	theme	pathway	1372:1378	arg1	analysis					1380:1387	Ingenuity pathway analysis	1362:1387	Ingenuity pathway analysis	1362:1387	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	7	35	theme	several	1421:1427	arg1	pathways					1439:1446	several signaling pathways	1421:1446	several signaling pathways	1421:1446	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	4	36	theme	lipopolysaccharide-induced	834:859	arg1	peritonitis					861:871	lipopolysaccharide-induced peritonitis	834:871	lipopolysaccharide-induced peritonitis	834:871	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	2	37	theme	efficacious	475:485	arg1	doses					487:491	the efficacious doses	471:491	the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects	471:566	However, the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects are not fully elucidated.
37403713	0	38	theme	anti-inflammatory	15:31	arg1	mechanisms					33:42	the anti-inflammatory mechanisms	11:42	the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice	11:133	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	8	39	theme	inflammatory	1698:1709	arg1	markers					1711:1717	inflammatory markers	1698:1717	inflammatory markers	1698:1717	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	0	40	from	peritonitis	115:125	arg1	mice					130:133	mice	130:133	mice	130:133	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	7	41	theme	signaling	1429:1437	arg1	pathways					1439:1446	several signaling pathways	1421:1446	several signaling pathways	1421:1446	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	2	42	theme	molecular	508:516	arg1	targets					518:524	its molecular targets	504:524	its molecular targets involved in the anti-inflammatory effects	504:566	However, the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects are not fully elucidated.
37403713	1	43	theme	phytochemical	241:253	arg1	composition					255:265	phytochemical composition	241:265	phytochemical composition	241:265	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	0	44	theme	phenolic-enriched	47:63	arg1	extract					77:83	phenolic-enriched maple syrup extract	47:83	phenolic-enriched maple syrup extract	47:83	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	5	45	theme	peritonitis	1190:1200	arg1	group					1202:1206	the peritonitis group	1186:1206	the peritonitis group	1186:1206	Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments.
37403713	0	46	theme	proteomics	179:188	arg1	analysis					190:197	data-independent acquisition proteomics analysis	150:197	data-independent acquisition proteomics analysis	150:197	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	3	47	theme	mouse	639:643	arg1	model					645:649	a peritonitis mouse model	625:649	a peritonitis mouse model	625:649	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	2	48	theme	targets	518:524	arg1	doses					487:491	the efficacious doses	471:491	the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects	471:566	However, the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects are not fully elucidated.
37403713	7	49	theme	activation	1498:1507	arg1	processes					1455:1463	the processes	1451:1463	the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis	1451:1570	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	0	50	theme	acquisition	167:177	arg1	analysis					190:197	data-independent acquisition proteomics analysis	150:197	data-independent acquisition proteomics analysis	150:197	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	3	51	theme	dose-finding	670:681	arg1	study					683:687	a dose-finding study	668:687	a dose-finding study	668:687	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	8	52	dep	in	1603:1604	arg1	vivo					1606:1609	vivo	1606:1609	vivo	1606:1609	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	4	53	theme	factor	989:994	arg1	TNF-α					1003:1007	TNF-α	1003:1007	TNF-α	1003:1007	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	53	theme	factor	989:994	arg1	alpha					996:1000	tumor necrosis factor alpha	974:1000	tumor necrosis factor alpha (TNF-α)	974:1008	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	0	54	theme	syrup	71:75	arg1	extract					77:83	phenolic-enriched maple syrup extract	47:83	phenolic-enriched maple syrup extract	47:83	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	8	55	theme	in	1603:1604	arg1	findings					1611:1618	these proteomic and in vivo findings	1583:1618	these proteomic and in vivo findings	1583:1618	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	6	56	theme	interferon	1336:1345	arg1	gamma					1347:1351	interferon gamma	1336:1351	interferon gamma	1336:1351	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	2	57	theme	anti-inflammatory	542:558	arg1	effects					560:566	the anti-inflammatory effects	538:566	the anti-inflammatory effects	538:566	However, the efficacious doses of MSX and its molecular targets involved in the anti-inflammatory effects are not fully elucidated.
37403713	5	58	theme	proteins	1112:1119	arg1	panel					1103:1107	a panel	1101:1107	a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments	1101:1253	Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments.
37403713	0	59	theme	maple	65:69	arg1	extract					77:83	phenolic-enriched maple syrup extract	47:83	phenolic-enriched maple syrup extract	47:83	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	1	60	theme	promising	356:364	arg1	effects					384:390	promising anti-inflammatory effects	356:390	promising anti-inflammatory effects	356:390	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	3	61	theme	acquisition	756:766	arg1	assay					785:789	data-independent acquisition (DIA) proteomics assay	739:789	data-independent acquisition (DIA) proteomics assay	739:789	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	7	62	theme	suppression	1536:1546	arg1	processes					1455:1463	the processes	1451:1463	the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis	1451:1570	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	3	63	theme	peritonitis	627:637	arg1	model					645:649	a peritonitis mouse model	625:649	a peritonitis mouse model	625:649	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	4	64	dep	serum	1017:1021	arg1	the					1013:1015	the	1013:1015	the	1013:1015	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	3	65	from	efficacy	606:613	arg1	model					645:649	a peritonitis mouse model	625:649	a peritonitis mouse model	625:649	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	1	66	theme	anti-inflammatory	366:382	arg1	effects					384:390	promising anti-inflammatory effects	356:390	promising anti-inflammatory effects	356:390	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	8	67	theme	inflammation	1653:1664	arg1	pathways					1676:1683	inflammation signaling pathways	1653:1683	inflammation signaling pathways	1653:1683	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	4	68	theme	mg	814:815	arg1	kg-1					817:820	at 15, 30 and 60 mg kg-1	797:820	at 15, 30 and 60 mg kg-1	797:820	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	4	68	theme	mg	814:815	arg1	MSX					792:794	MSX	792:794	MSX (at 15, 30 and 60 mg kg-1)	792:821	MSX (at 15, 30 and 60 mg kg-1) alleviated lipopolysaccharide-induced peritonitis by reducing the levels of pro-inflammatory cytokines including interleukin-1 beta (IL-1β), IL-6, and tumor necrosis factor alpha (TNF-α) in the serum and major organs of the mice.
37403713	1	69	dep	composition	255:265	arg1	the					237:239	the	237:239	the	237:239	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	1	70	theme	biological	271:280	arg1	activities					282:291	biological activities	271:291	biological activities	271:291	Our group has previously reported on the phytochemical composition and biological activities of a phenolic-enriched maple syrup extract (MSX), which showed promising anti-inflammatory effects in several disease models including diabetes and Alzheimer's disease.
37403713	6	71	theme	upstream	1306:1313	arg1	TNF					1357:1359	TNF	1357:1359	TNF	1357:1359	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	6	71	theme	upstream	1306:1313	arg1	regulators					1315:1324	several inflammatory upstream regulators	1285:1324	several inflammatory upstream regulators including interferon gamma and TNF	1285:1359	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	6	71	theme	upstream	1306:1313	arg1	gamma					1347:1351	interferon gamma	1336:1351	interferon gamma	1336:1351	MSX treatment also modulated several inflammatory upstream regulators including interferon gamma and TNF.
37403713	3	72	theme	underlying	697:706	arg1	mechanisms					708:717	the underlying mechanisms	693:717	the underlying mechanisms	693:717	Herein, the efficacy of MSX in a peritonitis mouse model was evaluated in a dose-finding study and the underlying mechanisms were explored using data-independent acquisition (DIA) proteomics assay.
37403713	8	73	theme	proteomic	1589:1597	arg1	findings					1611:1618	these proteomic and in vivo findings	1583:1618	these proteomic and in vivo findings	1583:1618	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	5	74	theme	DIA	1066:1068	arg1	analyses					1081:1088	DIA proteomics analyses	1066:1088	DIA proteomics analyses	1066:1088	Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments.
37403713	7	75	theme	Ingenuity	1362:1370	arg1	analysis					1380:1387	Ingenuity pathway analysis	1362:1387	Ingenuity pathway analysis	1362:1387	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	0	76	theme	extract	77:83	arg1	mechanisms					33:42	the anti-inflammatory mechanisms	11:42	the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice	11:133	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	0	77	from	mechanisms	33:42	arg1	peritonitis					115:125	lipopolysaccharide-induced peritonitis	88:125	lipopolysaccharide-induced peritonitis in mice	88:133	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	0	78	dep	Uncovering	0:9	arg1	insights					136:143	insights	136:143	insights from data-independent acquisition proteomics analysis	136:197	Uncovering the anti-inflammatory mechanisms of phenolic-enriched maple syrup extract in lipopolysaccharide-induced peritonitis in mice: insights from data-independent acquisition proteomics analysis.
37403713	7	79	theme	hepatocyte	1551:1560	arg1	apoptosis					1562:1570	hepatocyte apoptosis	1551:1570	hepatocyte apoptosis	1551:1570	Ingenuity pathway analysis suggested that MSX may modulate several signaling pathways in the processes of initiation of cytokine storm, activation of liver regeneration, and suppression of hepatocyte apoptosis.
37403713	8	80	theme	signaling	1666:1674	arg1	pathways					1676:1683	inflammation signaling pathways	1653:1683	inflammation signaling pathways	1653:1683	Together, these proteomic and in vivo findings indicate that MSX could regulate inflammation signaling pathways and modulate inflammatory markers and proteins, providing critical insight to its therapeutic potential.
37403713	5	81	theme	MSX	1240:1242	arg1	treatments					1244:1253	the MSX treatments	1236:1253	the MSX treatments	1236:1253	Furthermore, DIA proteomics analyses identified a panel of proteins that were significantly altered (both up- and down-regulated) in the peritonitis group, which were counteracted by the MSX treatments.
36939991	4	0	theme	designed	776:783	arg1	analogues					785:793	The designed analogues	772:793	The designed analogues	772:793	The designed analogues were efficiently synthesized by tuning the protecting group of the glucosylsphingosine acceptor.
36939991	1	1	theme	functional	197:206	arg1	group					208:212	a bioorthogonal functional group	181:212	a bioorthogonal functional group due to its compactness and nonpolar structure	181:258	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	1	1	theme	functional	197:206	arg1	tag					126:128	The alkyne tag	115:128	The alkyne tag	115:128	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	2	2	theme	alkyne	418:423	arg1	tag					425:427	an alkyne tag	415:427	an alkyne tag in the fatty acid moiety	415:452	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	3	3	theme	glycan	596:601	arg1	chain					603:607	the glycan chain	592:607	the glycan chain	592:607	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	4	link	-CHF-linked	722:732	arg1	S					720:720	S	720:720	S	720:720	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	4	link	-CHF-linked	722:732	arg1	analogues					738:746	sialidase-resistant (S)-CHF-linked GM3 analogues	699:746	sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group	699:769	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	5	5	theme	tag	1021:1023	arg1	position					998:1005	the position	994:1005	the position of the alkyne tag	994:1023	The growth-promoting effect of these analogues on Had-1 cells was dramatically altered depending upon the position of the alkyne tag.
36939991	3	6	theme	factors	561:567	arg1	influence					542:550	the influence	538:550	the influence of other factors such as degradation of the glycan chain	538:607	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	0	7	theme	-CHF-Sialoside	73:86	arg1	linkage					88:94	a (S)-CHF-Sialoside linkage	68:94	a (S)-CHF-Sialoside linkage	68:94	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	3	8	theme	chain	603:607	arg1	degradation					577:587	degradation	577:587	degradation of the glycan chain	577:607	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	2	9	theme	biological	504:513	arg1	activity					515:522	the biological activity	500:522	the biological activity	500:522	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	1	10	theme	due	214:216	arg1	group					208:212	a bioorthogonal functional group	181:212	a bioorthogonal functional group due to its compactness and nonpolar structure	181:258	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	1	10	theme	due	214:216	arg1	tag					126:128	The alkyne tag	115:128	The alkyne tag	115:128	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	5	11	theme	growth-promoting	896:911	arg1	effect					913:918	The growth-promoting effect	892:918	The growth-promoting effect of these analogues on Had-1 cells	892:952	The growth-promoting effect of these analogues on Had-1 cells was dramatically altered depending upon the position of the alkyne tag.
36939991	3	12	theme	GM3	734:736	arg1	S					720:720	S	720:720	S	720:720	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	12	theme	GM3	734:736	arg1	analogues					738:746	sialidase-resistant (S)-CHF-linked GM3 analogues	699:746	sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group	699:769	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	2	13	theme	acid	442:445	arg1	moiety					447:452	the fatty acid moiety	432:452	the fatty acid moiety	432:452	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	2	14	theme	fatty	436:440	arg1	moiety					447:452	the fatty acid moiety	432:452	the fatty acid moiety	432:452	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	1	15	theme	alkyne	312:317	arg1	tag					319:321	an alkyne tag	309:321	an alkyne tag	309:321	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	4	16	theme	protecting	838:847	arg1	acceptor					882:889	the glucosylsphingosine acceptor	858:889	the glucosylsphingosine acceptor	858:889	The designed analogues were efficiently synthesized by tuning the protecting group of the glucosylsphingosine acceptor.
36939991	4	16	theme	protecting	838:847	arg1	group					849:853	the protecting group	834:853	the protecting group of the glucosylsphingosine acceptor	834:889	The designed analogues were efficiently synthesized by tuning the protecting group of the glucosylsphingosine acceptor.
36939991	0	17	theme	alkyne	103:108	arg1	tag					110:112	an alkyne tag	100:112	an alkyne tag	100:112	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	0	18	theme	biological	14:23	arg1	activity					25:32	biological activity	14:32	biological activity	14:32	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	3	19	theme	cellular	650:657	arg1	environment					659:669	a cellular environment	648:669	a cellular environment	648:669	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	2	20	from	tag	425:427	arg1	moiety					447:452	the fatty acid moiety	432:452	the fatty acid moiety	432:452	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	0	21	theme	ganglioside	37:47	arg1	GM3					49:51	ganglioside GM3	37:51	ganglioside GM3	37:51	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	1	22	theme	nonpolar	241:248	arg1	structure					250:258	nonpolar structure	241:258	nonpolar structure	241:258	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	5	23	theme	Had-1	942:946	arg1	cells					948:952	Had-1 cells	942:952	Had-1 cells	942:952	The growth-promoting effect of these analogues on Had-1 cells was dramatically altered depending upon the position of the alkyne tag.
36939991	5	24	theme	alkyne	1014:1019	arg1	tag					1021:1023	the alkyne tag	1010:1023	the alkyne tag	1010:1023	The growth-promoting effect of these analogues on Had-1 cells was dramatically altered depending upon the position of the alkyne tag.
36939991	4	25	theme	acceptor	882:889	arg1	acceptor					882:889	the glucosylsphingosine acceptor	858:889	the glucosylsphingosine acceptor	858:889	The designed analogues were efficiently synthesized by tuning the protecting group of the glucosylsphingosine acceptor.
36939991	4	25	theme	acceptor	882:889	arg1	group					849:853	the protecting group	834:853	the protecting group of the glucosylsphingosine acceptor	834:889	The designed analogues were efficiently synthesized by tuning the protecting group of the glucosylsphingosine acceptor.
36939991	2	26	theme	GM3	403:405	arg1	analogues					378:386	analogues	378:386	analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety	378:452	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	4	27	theme	glucosylsphingosine	862:880	arg1	acceptor					882:889	the glucosylsphingosine acceptor	858:889	the glucosylsphingosine acceptor	858:889	The designed analogues were efficiently synthesized by tuning the protecting group of the glucosylsphingosine acceptor.
36939991	3	28	theme	other	555:559	arg1	factors					561:567	other factors	555:567	other factors such as degradation of the glycan chain	555:607	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	28	theme	other	555:559	arg1	degradation					577:587	degradation	577:587	degradation of the glycan chain	577:607	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	29	theme	biological	625:634	arg1	activity					636:643	biological activity	625:643	biological activity	625:643	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	0	30	theme	GM3	49:51	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	0	30	theme	GM3	49:51	arg1	activity					25:32	biological activity	14:32	biological activity	14:32	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	2	31	theme	tag	493:495	arg1	effect					472:477	the effect	468:477	the effect of the alkyne tag on the biological activity	468:522	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	2	32	theme	ganglioside	391:401	arg1	GM3					403:405	ganglioside GM3	391:405	ganglioside GM3 bearing an alkyne tag in the fatty acid moiety	391:452	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	1	33	theme	various	265:271	arg1	probes					273:278	various probes	265:278	various probes consisting of lipids bearing an alkyne tag	265:321	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	2	34	theme	alkyne	486:491	arg1	tag					493:495	the alkyne tag	482:495	the alkyne tag	482:495	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	5	35	theme	analogues	929:937	arg1	effect					913:918	The growth-promoting effect	892:918	The growth-promoting effect of these analogues on Had-1 cells	892:952	The growth-promoting effect of these analogues on Had-1 cells was dramatically altered depending upon the position of the alkyne tag.
36939991	0	36	with	analogues	53:61	arg1	tag					110:112	an alkyne tag	100:112	an alkyne tag	100:112	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	0	36	with	analogues	53:61	arg1	linkage					88:94	a (S)-CHF-Sialoside linkage	68:94	a (S)-CHF-Sialoside linkage	68:94	Synthesis and biological activity of ganglioside GM3 analogues with a (S)-CHF-Sialoside linkage and an alkyne tag.
36939991	1	37	used	used	173:176	arg2	group					208:212	a bioorthogonal functional group	181:212	a bioorthogonal functional group due to its compactness and nonpolar structure	181:258	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	1	37	used	used	173:176	arg2	tag					126:128	The alkyne tag	115:128	The alkyne tag	115:128	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	3	38	theme	sialidase-resistant	699:717	arg1	S					720:720	S	720:720	S	720:720	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	38	theme	sialidase-resistant	699:717	arg1	analogues					738:746	sialidase-resistant (S)-CHF-linked GM3 analogues	699:746	sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group	699:769	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	39	theme	-CHF-linked	722:732	arg1	S					720:720	S	720:720	S	720:720	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	3	39	theme	-CHF-linked	722:732	arg1	analogues					738:746	sialidase-resistant (S)-CHF-linked GM3 analogues	699:746	sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group	699:769	To eliminate the influence of other factors such as degradation of the glycan chain when evaluating biological activity in a cellular environment, we introduced the tag into sialidase-resistant (S)-CHF-linked GM3 analogues developed by our group.
36939991	2	40	from	effect	472:477	arg1	activity					515:522	the biological activity	500:522	the biological activity	500:522	Here, we designed and synthesized analogues of ganglioside GM3 bearing an alkyne tag in the fatty acid moiety and evaluated the effect of the alkyne tag on the biological activity.
36939991	1	41	theme	alkyne	119:124	arg1	group					208:212	a bioorthogonal functional group	181:212	a bioorthogonal functional group due to its compactness and nonpolar structure	181:258	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	1	41	theme	alkyne	119:124	arg1	tag					126:128	The alkyne tag	115:128	The alkyne tag	115:128	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	5	42	from	effect	913:918	arg1	cells					948:952	Had-1 cells	942:952	Had-1 cells	942:952	The growth-promoting effect of these analogues on Had-1 cells was dramatically altered depending upon the position of the alkyne tag.
36939991	1	43	theme	bioorthogonal	183:195	arg1	group					208:212	a bioorthogonal functional group	181:212	a bioorthogonal functional group due to its compactness and nonpolar structure	181:258	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36939991	1	43	theme	bioorthogonal	183:195	arg1	tag					126:128	The alkyne tag	115:128	The alkyne tag	115:128	The alkyne tag, consisting of only two carbons, is widely used as a bioorthogonal functional group due to its compactness and nonpolar structure, and various probes consisting of lipids bearing an alkyne tag have been developed.
36810971	15	0	theme	intestinal	3119:3128	arg1	flora					3130:3134	intestinal flora	3119:3134	intestinal flora	3119:3134	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	4	1	theme	control	784:790	arg1	control					799:805	control	799:805	control	799:805	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	1	theme	control	784:790	arg1	group					792:796	control group	784:796	control group (control)	784:806	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	7	2	theme	tumor	1534:1538	arg1	interleukin					1448:1458	interleukin 1β	1448:1461	interleukin 1β (IL-1β)	1448:1469	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	2	theme	tumor	1534:1538	arg1	factor					1549:1554	tumor necrosis factor α	1534:1556	tumor necrosis factor α (TNF-α)	1534:1564	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	2	theme	tumor	1534:1538	arg1	TNF-α					1559:1563	TNF-α	1559:1563	TNF-α	1559:1563	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	11	3	theme	pro-inflammatory	2242:2257	arg1	IL-1β					2278:2282	IL-1β	2278:2282	IL-1β	2278:2282	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	3	theme	pro-inflammatory	2242:2257	arg1	TNF-α					2291:2295	TNF-α	2291:2295	TNF-α	2291:2295	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	3	theme	pro-inflammatory	2242:2257	arg1	TGF-β					2302:2306	TGF-β	2302:2306	TGF-β in lung tissue and serum	2302:2331	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	3	theme	pro-inflammatory	2242:2257	arg1	IL-6					2285:2288	IL-6	2285:2288	IL-6	2285:2288	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	3	theme	pro-inflammatory	2242:2257	arg1	factors					2259:2265	pro-inflammatory factors	2242:2265	pro-inflammatory factors	2242:2265	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	3	4	theme	capsule	609:615	arg1	effects					585:591	the effects	581:591	the effects of Qi-Long-Tian capsule and gut microbiota of PF mice	581:645	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	11	5	from	IL-1β	2278:2282	arg1	serum					2327:2331	serum	2327:2331	serum	2327:2331	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	5	from	IL-1β	2278:2282	arg1	tissue					2316:2321	lung tissue	2311:2321	lung tissue	2311:2321	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	12	6	from	composition	2565:2575	arg1	groups					2633:2638	the control, model, and QLT capsule groups	2597:2638	groups	2633:2638	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	12	6	from	composition	2565:2575	arg1	model					2610:2614	the control, model, and QLT capsule groups	2597:2638	model	2610:2614	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	5	7	theme	pulmonary	996:1004	arg1	tests					1015:1019	pulmonary function tests	996:1019	pulmonary function tests	996:1019	After 21 days of treatment, after pulmonary function tests, the lung tissues, serums, and enterobacterial samples were collected for further analysis.
36810971	6	8	used	used	1152:1155	arg2	staining					1116:1123	HE staining	1113:1123	HE staining	1113:1123	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	6	8	used	used	1152:1155	arg2	staining					1138:1145	Masson's staining	1129:1145	Masson's staining	1129:1145	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	2	9	theme	clinical	511:518	arg1	practice					520:527	clinical practice	511:527	clinical practice	511:527	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	13	10	theme	relative	2794:2801	arg1	abundance					2803:2811	the relative abundance	2790:2811	the relative abundance of Clostridia (which might promote inflammation)	2790:2860	QLT capsule significantly increased the relative abundance of Bacteroidia (which might limit the onset of inflammation) and decreased the relative abundance of Clostridia (which might promote inflammation).
36810971	3	11	theme	mice	642:645	arg1	effects					585:591	the effects	581:591	the effects of Qi-Long-Tian capsule and gut microbiota of PF mice	581:645	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	12	12	theme	capsule	2625:2631	arg1	groups					2633:2638	the control, model, and QLT capsule groups	2597:2638	groups	2633:2638	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	8	13	theme	colonic	1843:1849	arg1	tissues					1851:1857	colonic tissues	1843:1857	colonic tissues	1843:1857	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	7	14	dep	protein	1656:1662	arg1	Occludin					1680:1687	Occludin	1680:1687	Occludin	1680:1687	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	14	dep	protein	1656:1662	arg1	Claudin					1671:1677	Claudin	1671:1677	Claudin	1671:1677	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	14	dep	protein	1656:1662	arg1	ZO-1					1665:1668	ZO-1	1665:1668	ZO-1	1665:1668	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	15	15	theme	mucosal	3199:3205	arg1	barrier					3207:3213	the intestinal mucosal barrier	3184:3213	the intestinal mucosal barrier	3184:3213	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	11	16	from	TGF-β	2302:2306	arg1	serum					2327:2331	serum	2327:2331	serum	2327:2331	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	16	from	TGF-β	2302:2306	arg1	tissue					2316:2321	lung tissue	2311:2321	lung tissue	2311:2321	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	12	17	from	diversity	2518:2526	arg1	enterobacteria					2531:2544	enterobacteria	2531:2544	enterobacteria	2531:2544	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	2	18	theme	great	290:294	arg1	potential					296:304	great potential	290:304	great potential	290:304	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	6	19	theme	collagen	1273:1280	arg1	metabolism					1282:1291	collagen metabolism	1273:1291	collagen metabolism	1273:1291	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	1	20	theme	interstitial	183:194	arg1	disease					201:207	a chronic, progressive, and fibrotic interstitial lung disease	146:207	a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate	146:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	1	20	theme	interstitial	183:194	arg1	fibrosis					129:136	Pulmonary fibrosis	119:136	Pulmonary fibrosis (PF)	119:141	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	7	21	theme	protein	1392:1398	arg1	expressions					1400:1410	the mRNA and protein expressions	1379:1410	expressions	1400:1410	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	22	theme	transforming	1494:1505	arg1	interleukin					1448:1458	interleukin 1β	1448:1461	interleukin 1β (IL-1β)	1448:1469	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	22	theme	transforming	1494:1505	arg1	factor					1514:1519	transforming growth factor β1	1494:1522	transforming growth factor β1 (TGF-β1)	1494:1531	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	22	theme	transforming	1494:1505	arg1	TGF-β1					1525:1530	TGF-β1	1525:1530	TGF-β1	1525:1530	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	11	23	theme	related	2381:2387	arg1	Occludin					2412:2419	Occludin	2412:2419	Occludin	2412:2419	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	23	theme	related	2381:2387	arg1	factors					2389:2395	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	23	theme	related	2381:2387	arg1	sIgA					2422:2425	sIgA	2422:2425	sIgA	2422:2425	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	23	theme	related	2381:2387	arg1	LPS					2448:2450	reducing LPS	2439:2450	reducing LPS	2439:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	23	theme	related	2381:2387	arg1	ZO-1					2397:2400	ZO-1	2397:2400	ZO-1	2397:2400	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	23	theme	related	2381:2387	arg1	Claudin					2403:2409	Claudin	2403:2409	Claudin	2403:2409	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	23	theme	related	2381:2387	arg1	SCFAs					2428:2432	SCFAs	2428:2432	SCFAs	2428:2432	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	3	24	theme	pulmonary	648:656	arg1	fibrosis					658:665	pulmonary fibrosis	648:665	pulmonary fibrosis model	648:671	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	6	25	theme	related	1262:1268	arg1	hydroxyproline					1241:1254	hydroxyproline	1241:1254	hydroxyproline (HYP) related to collagen metabolism	1241:1291	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	6	25	theme	related	1262:1268	arg1	HYP					1257:1259	HYP	1257:1259	HYP	1257:1259	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	7	26	theme	mRNA	1383:1386	arg1	expressions					1400:1410	the mRNA and protein expressions	1379:1410	expressions	1400:1410	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	9	27	from	changes	1902:1908	arg1	model					1977:1981	the control, model, and QM groups	1964:1996	model	1977:1981	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	9	27	from	changes	1902:1908	arg1	diversity					1931:1939	diversity	1931:1939	diversity	1931:1939	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	9	27	from	changes	1902:1908	arg1	abundance					1917:1925	abundance	1917:1925	abundance	1917:1925	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	9	27	from	changes	1902:1908	arg1	groups					1991:1996	the control, model, and QM groups	1964:1996	groups	1991:1996	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	14	28	theme	pro-inflammatory	2970:2985	arg1	factors					2987:2993	pro-inflammatory factors	2970:2993	pro-inflammatory factors	2970:2993	In addition, these two enterobacteria were closely associated with pro-inflammatory-related indicators and pro-inflammatory factors in PF.
36810971	7	29	from	expressions	1400:1410	arg1	tissues					1574:1580	lung tissues	1569:1580	lung tissues	1569:1580	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	29	from	expressions	1400:1410	arg1	serums					1586:1591	serums	1586:1591	serums	1586:1591	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	10	30	theme	pulmonary	2136:2144	arg1	fibrosis					2146:2153	pulmonary fibrosis	2136:2153	pulmonary fibrosis	2136:2153	QLT capsule effectively improved the status of pulmonary fibrosis and reduced HYP.
36810971	3	31	theme	tracheal	693:700	arg1	injection					707:715	tracheal drip injection	693:715	tracheal drip injection of bleomycin	693:728	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	6	32	theme	HE	1113:1114	arg1	staining					1116:1123	HE staining	1113:1123	HE staining	1113:1123	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	15	33	theme	immunoglobulin	3148:3161	arg1	secretion					3163:3171	immunoglobulin secretion	3148:3171	immunoglobulin secretion	3148:3171	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	9	34	dep	abundance	1917:1925	arg1	the					1913:1915	the	1913:1915	the	1913:1915	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	9	35	theme	differential	2013:2024	arg1	genera					2026:2031	differential genera	2013:2031	differential genera	2013:2031	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	9	36	theme	16sRNA	1860:1865	arg1	sequencing					1872:1881	16sRNA gene sequencing	1860:1881	16sRNA gene sequencing	1860:1881	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	9	37	theme	inflammatory	2067:2078	arg1	factors					2080:2086	inflammatory factors	2067:2086	inflammatory factors	2067:2086	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	4	38	theme	model	809:813	arg1	model					822:826	model	822:826	model	822:826	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	38	theme	model	809:813	arg1	group					815:819	model group	809:819	model group (model)	809:827	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	17	39	theme	theoretical	3482:3492	arg1	basis					3494:3498	a theoretical basis	3480:3498	a theoretical basis for its further clinical application	3480:3535	It provides a theoretical basis for its further clinical application.
36810971	3	40	theme	bleomycin	720:728	arg1	injection					707:715	tracheal drip injection	693:715	tracheal drip injection of bleomycin	693:728	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	2	41	theme	QLT	251:253	arg1	capsule					256:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	41	theme	QLT	251:253	arg1	formula					277:283	an herbal formula	267:283	an herbal formula	267:283	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	8	42	theme	protein	1720:1726	arg1	expressions					1728:1738	the protein expressions	1716:1738	the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues	1716:1857	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	0	43	theme	inflammatory	77:88	arg1	response					90:97	inflammatory response	77:97	inflammatory response	77:97	Qi-Long-Tian capsule alleviates pulmonary fibrosis development by modulating inflammatory response and gut microbiota.
36810971	6	44	theme	PF	1201:1202	arg1	indicators					1187:1196	the main indicators	1178:1196	the main indicators of PF	1178:1202	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	6	44	theme	PF	1201:1202	arg1	changes					1167:1173	changes	1167:1173	changes	1167:1173	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	6	45	theme	alkaline	1313:1320	arg1	method					1333:1338	and alkaline hydrolysis method	1309:1338	method	1333:1338	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	1	46	theme	chronic	148:154	arg1	disease					201:207	a chronic, progressive, and fibrotic interstitial lung disease	146:207	a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate	146:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	1	46	theme	chronic	148:154	arg1	fibrosis					129:136	Pulmonary fibrosis	119:136	Pulmonary fibrosis (PF)	119:141	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	16	47	from	PF	3424:3425	arg1	mechanism					3396:3404	the therapeutic mechanism	3380:3404	the therapeutic mechanism of QLT capsule in PF	3380:3425	This study clarifies the therapeutic mechanism of QLT capsule in PF and provides a theoretical basis for it.
36810971	12	48	theme	control	2601:2607	arg1	model					2610:2614	the control, model, and QLT capsule groups	2597:2638	model	2610:2614	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	7	49	dep	factors	1432:1438	arg1	include					1440:1446	include	1440:1446	include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α)	1440:1564	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	17	50	theme	further	3508:3514	arg1	application					3525:3535	its further clinical application	3504:3535	its further clinical application	3504:3535	It provides a theoretical basis for its further clinical application.
36810971	1	51	theme	progressive	157:167	arg1	disease					201:207	a chronic, progressive, and fibrotic interstitial lung disease	146:207	a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate	146:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	1	51	theme	progressive	157:167	arg1	fibrosis					129:136	Pulmonary fibrosis	119:136	Pulmonary fibrosis (PF)	119:141	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	2	52	with	formula	277:283	arg1	potential					296:304	great potential	290:304	great potential	290:304	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	4	53	theme	QLT	830:832	arg1	group					851:855	QLT capsule low dose group	830:855	QLT capsule low dose group (QL)	830:860	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	53	theme	QLT	830:832	arg1	QL					858:859	QL	858:859	QL	858:859	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	16	54	from	capsule	3413:3419	arg1	PF					3424:3425	PF	3424:3425	PF	3424:3425	This study clarifies the therapeutic mechanism of QLT capsule in PF and provides a theoretical basis for it.
36810971	9	55	used	used	1887:1890	arg2	sequencing					1872:1881	16sRNA gene sequencing	1860:1881	16sRNA gene sequencing	1860:1881	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	4	56	theme	low	842:844	arg1	group					851:855	QLT capsule low dose group	830:855	QLT capsule low dose group (QL)	830:860	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	56	theme	low	842:844	arg1	QL					858:859	QL	858:859	QL	858:859	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	12	57	theme	beta	2513:2516	arg1	diversity					2518:2526	beta diversity	2513:2526	beta diversity	2513:2526	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	2	58	dep	Qi	350:351	arg1	Perrier					420:426	Perrier	420:426	Perrier	420:426	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	58	dep	Qi	350:351	arg1	Notoginseng					354:364	Notoginseng	354:364	Notoginseng	354:364	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	58	dep	Qi	350:351	arg1	Rhizoma					375:381	Rhizoma	375:381	Rhizoma	375:381	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	58	dep	Qi	350:351	arg1	Di					385:386	Di	385:386	Di	385:386	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	7	59	theme	tight	1641:1645	arg1	protein					1656:1662	tight junction protein	1641:1662	tight junction protein (ZO-1, Claudin, Occludin)	1641:1688	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	15	60	theme	pulmonary	3059:3067	arg1	fibrosis					3069:3076	pulmonary fibrosis	3059:3076	pulmonary fibrosis	3059:3076	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	8	61	theme	immunoglobulin	1753:1766	arg1	sIgA					1771:1774	sIgA	1771:1774	sIgA	1771:1774	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	8	61	theme	immunoglobulin	1753:1766	arg1	A					1768:1768	secretory immunoglobulin A	1743:1768	secretory immunoglobulin A (sIgA)	1743:1775	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	4	62	theme	dose	882:885	arg1	QM					894:895	QM	894:895	QM	894:895	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	62	theme	dose	882:885	arg1	group					887:891	QLT capsule medium dose group	863:891	QLT capsule medium dose group (QM)	863:896	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	0	63	theme	Qi-Long-Tian	0:11	arg1	capsule					13:19	Qi-Long-Tian capsule	0:19	Qi-Long-Tian capsule	0:19	Qi-Long-Tian capsule alleviates pulmonary fibrosis development by modulating inflammatory response and gut microbiota.
36810971	11	64	theme	QLT	2185:2187	arg1	capsule					2189:2195	QLT capsule	2185:2195	QLT capsule	2185:2195	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	7	65	theme	pro-inflammatory	1415:1430	arg1	factors					1432:1438	pro-inflammatory factors	1415:1438	pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α)	1415:1564	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	2	66	theme	[Pheretima	393:402	arg1	aspergillum					404:414	Long [Pheretima aspergillum	388:414	Di Long [Pheretima aspergillum (E. Perrier)	385:427	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	5	67	theme	further	1095:1101	arg1	analysis					1103:1110	further analysis	1095:1110	further analysis	1095:1110	After 21 days of treatment, after pulmonary function tests, the lung tissues, serums, and enterobacterial samples were collected for further analysis.
36810971	9	68	theme	intestinal	1944:1953	arg1	flora					1955:1959	intestinal flora	1944:1959	intestinal flora	1944:1959	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	4	69	dep	divided	761:767	arg1	QM					894:895	QM	894:895	QM	894:895	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	group					949:953	pirfenidone group	937:953	pirfenidone group (PFD)	937:959	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	group					851:855	QLT capsule low dose group	830:855	QLT capsule low dose group (QL)	830:860	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	control					799:805	control	799:805	control	799:805	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	QH					928:929	QH	928:929	QH	928:929	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	QL					858:859	QL	858:859	QL	858:859	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	PFD					956:958	PFD	956:958	PFD	956:958	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	group					887:891	QLT capsule medium dose group	863:891	QLT capsule medium dose group (QM)	863:896	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	model					822:826	model	822:826	model	822:826	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	group					792:796	control group	784:796	control group (control)	784:806	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	group					815:819	model group	809:819	model group (model)	809:827	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	69	dep	divided	761:767	arg1	group					921:925	QLT capsule high dose group	899:925	QLT capsule high dose group (QH)	899:930	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	5	70	theme	treatment	979:987	arg1	21 days					968:974	21 days	968:974	21 days of treatment	968:987	After 21 days of treatment, after pulmonary function tests, the lung tissues, serums, and enterobacterial samples were collected for further analysis.
36810971	15	71	theme	QLT	3033:3035	arg1	capsule					3037:3043	QLT capsule	3033:3043	QLT capsule	3033:3043	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	13	72	theme	Bacteroidia	2718:2728	arg1	abundance					2705:2713	the relative abundance	2692:2713	the relative abundance of Bacteroidia (which might limit the onset of inflammation)	2692:2774	QLT capsule significantly increased the relative abundance of Bacteroidia (which might limit the onset of inflammation) and decreased the relative abundance of Clostridia (which might promote inflammation).
36810971	8	73	used	used	1701:1704	arg2	ELISA					1691:1695	ELISA	1691:1695	ELISA	1691:1695	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	0	74	theme	fibrosis	42:49	arg1	development					51:61	pulmonary fibrosis development	32:61	pulmonary fibrosis development	32:61	Qi-Long-Tian capsule alleviates pulmonary fibrosis development by modulating inflammatory response and gut microbiota.
36810971	1	75	with	disease	201:207	arg1	rate					231:234	a high mortality rate	214:234	a high mortality rate	214:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	8	76	theme	short-chain	1778:1788	arg1	SCFAs					1803:1807	SCFAs	1803:1807	SCFAs	1803:1807	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	8	76	theme	short-chain	1778:1788	arg1	acids					1796:1800	short-chain fatty acids	1778:1800	short-chain fatty acids (SCFAs)	1778:1808	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	4	77	theme	capsule	867:873	arg1	QM					894:895	QM	894:895	QM	894:895	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	77	theme	capsule	867:873	arg1	group					887:891	QLT capsule medium dose group	863:891	QLT capsule medium dose group (QM)	863:896	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	2	78	dep	Di	385:386	arg1	aspergillum					404:414	Long [Pheretima aspergillum	388:414	Di Long [Pheretima aspergillum (E. Perrier)	385:427	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	12	79	from	diversity	2499:2507	arg1	enterobacteria					2531:2544	enterobacteria	2531:2544	enterobacteria	2531:2544	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	9	80	theme	control	1968:1974	arg1	model					1977:1981	the control, model, and QM groups	1964:1996	model	1977:1981	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	15	81	theme	factor	3279:3284	arg1	secretion					3286:3294	inflammatory factor secretion	3266:3294	inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation	3266:3356	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	8	82	theme	acids	1796:1800	arg1	expressions					1728:1738	the protein expressions	1716:1738	the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues	1716:1857	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	4	83	theme	high	911:914	arg1	QH					928:929	QH	928:929	QH	928:929	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	83	theme	high	911:914	arg1	group					921:925	QLT capsule high dose group	899:925	QLT capsule high dose group (QH)	899:930	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	2	84	theme	Qi-Long-Tian	237:248	arg1	capsule					256:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	84	theme	Qi-Long-Tian	237:248	arg1	formula					277:283	an herbal formula	267:283	an herbal formula	267:283	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	14	85	theme	pro-inflammatory-related	2930:2953	arg1	indicators					2955:2964	pro-inflammatory-related indicators	2930:2964	pro-inflammatory-related indicators	2930:2964	In addition, these two enterobacteria were closely associated with pro-inflammatory-related indicators and pro-inflammatory factors in PF.
36810971	3	86	theme	Qi-Long-Tian	596:607	arg1	capsule					609:615	Qi-Long-Tian capsule	596:615	Qi-Long-Tian capsule	596:615	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	2	87	dep	Notoginseng	354:364	arg1	et					372:373	Notoginseng Radix et	354:373	Notoginseng Radix et Rhizoma	354:381	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	88	theme	antifibrotic	310:321	arg1	effects					323:329	antifibrotic effects	310:329	antifibrotic effects	310:329	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	1	89	theme	high	216:219	arg1	rate					231:234	a high mortality rate	214:234	a high mortality rate	214:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	7	90	theme	lung	1569:1572	arg1	tissues					1574:1580	lung tissues	1569:1580	lung tissues	1569:1580	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	91	theme	necrosis	1540:1547	arg1	interleukin					1448:1458	interleukin 1β	1448:1461	interleukin 1β (IL-1β)	1448:1469	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	91	theme	necrosis	1540:1547	arg1	factor					1549:1554	tumor necrosis factor α	1534:1556	tumor necrosis factor α (TNF-α)	1534:1564	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	91	theme	necrosis	1540:1547	arg1	TNF-α					1559:1563	TNF-α	1559:1563	TNF-α	1559:1563	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	11	92	theme	abnormal	2223:2230	arg1	levels					2232:2237	the abnormal levels	2219:2237	the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum	2219:2331	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	5	93	theme	lung	1026:1029	arg1	tissues					1031:1037	the lung tissues	1022:1037	the lung tissues	1022:1037	After 21 days of treatment, after pulmonary function tests, the lung tissues, serums, and enterobacterial samples were collected for further analysis.
36810971	8	94	theme	secretory	1743:1751	arg1	sIgA					1771:1774	sIgA	1771:1774	sIgA	1771:1774	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	8	94	theme	secretory	1743:1751	arg1	A					1768:1768	secretory immunoglobulin A	1743:1768	secretory immunoglobulin A (sIgA)	1743:1775	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	13	95	theme	QLT	2656:2658	arg1	capsule					2660:2666	QLT capsule	2656:2666	QLT capsule	2656:2666	QLT capsule significantly increased the relative abundance of Bacteroidia (which might limit the onset of inflammation) and decreased the relative abundance of Clostridia (which might promote inflammation).
36810971	3	96	theme	microbiota	625:634	arg1	effects					585:591	the effects	581:591	the effects of Qi-Long-Tian capsule and gut microbiota of PF mice	581:645	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	15	97	theme	flora	3130:3134	arg1	genera					3109:3114	the differential genera	3092:3114	the differential genera of intestinal flora	3092:3134	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	8	98	theme	lipopolysaccharide	1815:1832	arg1	expressions					1728:1738	the protein expressions	1716:1738	the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues	1716:1857	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	4	99	theme	QLT	899:901	arg1	QH					928:929	QH	928:929	QH	928:929	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	99	theme	QLT	899:901	arg1	group					921:925	QLT capsule high dose group	899:925	QLT capsule high dose group (QH)	899:930	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	11	100	theme	factors	2259:2265	arg1	levels					2232:2237	the abnormal levels	2219:2237	the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum	2219:2331	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	2	101	theme	herbal	270:275	arg1	capsule					256:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	101	theme	herbal	270:275	arg1	formula					277:283	an herbal formula	267:283	an herbal formula	267:283	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	5	102	theme	function	1006:1013	arg1	tests					1015:1019	pulmonary function tests	996:1019	pulmonary function tests	996:1019	After 21 days of treatment, after pulmonary function tests, the lung tissues, serums, and enterobacterial samples were collected for further analysis.
36810971	4	103	theme	pirfenidone	937:947	arg1	PFD					956:958	PFD	956:958	PFD	956:958	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	103	theme	pirfenidone	937:947	arg1	group					949:953	pirfenidone group	937:953	pirfenidone group (PFD)	937:959	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	12	104	theme	alpha	2493:2497	arg1	diversity					2499:2507	alpha diversity	2493:2507	alpha diversity	2493:2507	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	11	105	from	TNF-α	2291:2295	arg1	serum					2327:2331	serum	2327:2331	serum	2327:2331	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	105	from	TNF-α	2291:2295	arg1	tissue					2316:2321	lung tissue	2311:2321	lung tissue	2311:2321	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	15	106	theme	differential	3096:3107	arg1	genera					3109:3114	the differential genera	3092:3114	the differential genera of intestinal flora	3092:3134	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	7	107	theme	growth	1507:1512	arg1	interleukin					1448:1458	interleukin 1β	1448:1461	interleukin 1β (IL-1β)	1448:1469	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	107	theme	growth	1507:1512	arg1	factor					1514:1519	transforming growth factor β1	1494:1522	transforming growth factor β1 (TGF-β1)	1494:1531	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	107	theme	growth	1507:1512	arg1	TGF-β1					1525:1530	TGF-β1	1525:1530	TGF-β1	1525:1530	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	3	108	theme	PF	639:640	arg1	mice					642:645	PF mice	639:645	PF mice	639:645	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	1	109	theme	fibrotic	174:181	arg1	disease					201:207	a chronic, progressive, and fibrotic interstitial lung disease	146:207	a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate	146:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	1	109	theme	fibrotic	174:181	arg1	fibrosis					129:136	Pulmonary fibrosis	119:136	Pulmonary fibrosis (PF)	119:141	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	16	110	theme	capsule	3413:3419	arg1	mechanism					3396:3404	the therapeutic mechanism	3380:3404	the therapeutic mechanism of QLT capsule in PF	3380:3425	This study clarifies the therapeutic mechanism of QLT capsule in PF and provides a theoretical basis for it.
36810971	2	111	theme	many	533:536	arg1	years					538:542	many years	533:542	many years	533:542	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	12	112	dep	diversity	2499:2507	arg1	the					2489:2491	the	2489:2491	the	2489:2491	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	12	113	theme	QLT	2621:2623	arg1	groups					2633:2638	the control, model, and QLT capsule groups	2597:2638	groups	2633:2638	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	1	114	theme	lung	196:199	arg1	disease					201:207	a chronic, progressive, and fibrotic interstitial lung disease	146:207	a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate	146:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	1	114	theme	lung	196:199	arg1	fibrosis					129:136	Pulmonary fibrosis	119:136	Pulmonary fibrosis (PF)	119:141	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	13	115	theme	Clostridia	2816:2825	arg1	abundance					2803:2811	the relative abundance	2790:2811	the relative abundance of Clostridia (which might promote inflammation)	2790:2860	QLT capsule significantly increased the relative abundance of Bacteroidia (which might limit the onset of inflammation) and decreased the relative abundance of Clostridia (which might promote inflammation).
36810971	14	116	from	indicators	2955:2964	arg1	PF					2998:2999	PF	2998:2999	PF	2998:2999	In addition, these two enterobacteria were closely associated with pro-inflammatory-related indicators and pro-inflammatory factors in PF.
36810971	10	117	theme	fibrosis	2146:2153	arg1	status					2126:2131	the status	2122:2131	the status of pulmonary fibrosis	2122:2153	QLT capsule effectively improved the status of pulmonary fibrosis and reduced HYP.
36810971	3	118	theme	fibrosis	658:665	arg1	model					667:671	pulmonary fibrosis model	648:671	pulmonary fibrosis model	648:671	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	11	119	theme	pro-inflammatory	2364:2379	arg1	Occludin					2412:2419	Occludin	2412:2419	Occludin	2412:2419	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	119	theme	pro-inflammatory	2364:2379	arg1	factors					2389:2395	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	119	theme	pro-inflammatory	2364:2379	arg1	sIgA					2422:2425	sIgA	2422:2425	sIgA	2422:2425	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	119	theme	pro-inflammatory	2364:2379	arg1	LPS					2448:2450	reducing LPS	2439:2450	reducing LPS	2439:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	119	theme	pro-inflammatory	2364:2379	arg1	ZO-1					2397:2400	ZO-1	2397:2400	ZO-1	2397:2400	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	119	theme	pro-inflammatory	2364:2379	arg1	Claudin					2403:2409	Claudin	2403:2409	Claudin	2403:2409	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	119	theme	pro-inflammatory	2364:2379	arg1	SCFAs					2428:2432	SCFAs	2428:2432	SCFAs	2428:2432	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	3	120	theme	drip	702:705	arg1	injection					707:715	tracheal drip injection	693:715	tracheal drip injection of bleomycin	693:728	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	16	121	theme	theoretical	3442:3452	arg1	basis					3454:3458	a theoretical basis	3440:3458	a theoretical basis for it	3440:3465	This study clarifies the therapeutic mechanism of QLT capsule in PF and provides a theoretical basis for it.
36810971	7	122	used	used	1364:1367	arg2	ELISA					1353:1357	ELISA	1353:1357	ELISA	1353:1357	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	7	122	used	used	1364:1367	arg2	qRT-PCR					1341:1347	qRT-PCR	1341:1347	qRT-PCR	1341:1347	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	11	123	theme	lung	2311:2314	arg1	tissue					2316:2321	lung tissue	2311:2321	lung tissue	2311:2321	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	5	124	theme	enterobacterial	1052:1066	arg1	samples					1068:1074	enterobacterial samples	1052:1074	enterobacterial samples	1052:1074	After 21 days of treatment, after pulmonary function tests, the lung tissues, serums, and enterobacterial samples were collected for further analysis.
36810971	9	125	theme	gene	1867:1870	arg1	sequencing					1872:1881	16sRNA gene sequencing	1860:1881	16sRNA gene sequencing	1860:1881	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	13	126	theme	inflammation	2762:2773	arg1	onset					2753:2757	the onset	2749:2757	the onset of inflammation	2749:2773	QLT capsule significantly increased the relative abundance of Bacteroidia (which might limit the onset of inflammation) and decreased the relative abundance of Clostridia (which might promote inflammation).
36810971	12	127	theme	flora	2588:2592	arg1	different					2645:2653	different	2645:2653	different	2645:2653	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	12	127	theme	flora	2588:2592	arg1	composition					2565:2575	the composition	2561:2575	the composition of the gut flora in the control, model, and QLT capsule groups	2561:2638	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	6	128	theme	hydrolysis	1322:1331	arg1	method					1333:1338	and alkaline hydrolysis method	1309:1338	method	1333:1338	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	4	129	theme	dose	846:849	arg1	group					851:855	QLT capsule low dose group	830:855	QLT capsule low dose group (QL)	830:860	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	129	theme	dose	846:849	arg1	QL					858:859	QL	858:859	QL	858:859	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	11	130	dep	factors	2389:2395	arg1	Occludin					2412:2419	Occludin	2412:2419	Occludin	2412:2419	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	130	dep	factors	2389:2395	arg1	factors					2389:2395	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	130	dep	factors	2389:2395	arg1	sIgA					2422:2425	sIgA	2422:2425	sIgA	2422:2425	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	130	dep	factors	2389:2395	arg1	LPS					2448:2450	reducing LPS	2439:2450	reducing LPS	2439:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	130	dep	factors	2389:2395	arg1	ZO-1					2397:2400	ZO-1	2397:2400	ZO-1	2397:2400	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	130	dep	factors	2389:2395	arg1	Claudin					2403:2409	Claudin	2403:2409	Claudin	2403:2409	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	130	dep	factors	2389:2395	arg1	SCFAs					2428:2432	SCFAs	2428:2432	SCFAs	2428:2432	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	2	131	used	used	503:506	arg2	capsule					256:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule	237:262	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	2	131	used	used	503:506	arg2	formula					277:283	an herbal formula	267:283	an herbal formula	267:283	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	0	132	theme	gut	103:105	arg1	microbiota					107:116	gut microbiota	103:116	gut microbiota	103:116	Qi-Long-Tian capsule alleviates pulmonary fibrosis development by modulating inflammatory response and gut microbiota.
36810971	11	133	from	IL-6	2285:2288	arg1	serum					2327:2331	serum	2327:2331	serum	2327:2331	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	133	from	IL-6	2285:2288	arg1	tissue					2316:2321	lung tissue	2311:2321	lung tissue	2311:2321	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	12	134	theme	gut	2584:2586	arg1	flora					2588:2592	the gut flora	2580:2592	the gut flora	2580:2592	The comparison between the alpha diversity and beta diversity in enterobacteria suggested that the composition of the gut flora in the control, model, and QLT capsule groups were different.
36810971	15	135	from	secretion	3286:3294	arg1	serum					3303:3307	the serum	3299:3307	the serum	3299:3307	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	17	136	theme	clinical	3516:3523	arg1	application					3525:3535	its further clinical application	3504:3535	its further clinical application	3504:3535	It provides a theoretical basis for its further clinical application.
36810971	7	137	theme	junction	1647:1654	arg1	protein					1656:1662	tight junction protein	1641:1662	tight junction protein (ZO-1, Claudin, Occludin)	1641:1688	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	11	138	theme	reducing	2439:2446	arg1	LPS					2448:2450	reducing LPS	2439:2450	reducing LPS	2439:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	11	138	theme	reducing	2439:2446	arg1	factors					2389:2395	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2364:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	15	139	theme	LPS	3225:3227	arg1	entry					3229:3233	LPS entry	3225:3233	LPS entry into the blood	3225:3248	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	11	140	theme	factors	2389:2395	arg1	levels					2354:2359	the levels	2350:2359	the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS	2350:2450	In addition, QLT capsule significantly reduced the abnormal levels of pro-inflammatory factors, including IL-1β, IL-6, TNF-α, and TGF-β in lung tissue and serum, while improving the levels of pro-inflammatory related factors ZO-1, Claudin, Occludin, sIgA, SCFAs, and reducing LPS in the colon.
36810971	8	141	theme	A	1768:1768	arg1	expressions					1728:1738	the protein expressions	1716:1738	the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues	1716:1857	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	4	142	theme	capsule	834:840	arg1	group					851:855	QLT capsule low dose group	830:855	QLT capsule low dose group (QL)	830:860	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	142	theme	capsule	834:840	arg1	QL					858:859	QL	858:859	QL	858:859	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	8	143	from	expressions	1728:1738	arg1	tissues					1851:1857	colonic tissues	1843:1857	colonic tissues	1843:1857	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	15	144	theme	pulmonary	3335:3343	arg1	inflammation					3345:3356	pulmonary inflammation	3335:3356	pulmonary inflammation	3335:3356	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	7	145	theme	inflammation-mediating	1602:1623	arg1	factors					1625:1631	the inflammation-mediating factors	1598:1631	the inflammation-mediating factors	1598:1631	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	4	146	theme	medium	875:880	arg1	QM					894:895	QM	894:895	QM	894:895	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	146	theme	medium	875:880	arg1	group					887:891	QLT capsule medium dose group	863:891	QLT capsule medium dose group (QM)	863:896	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	0	147	theme	pulmonary	32:40	arg1	development					51:61	pulmonary fibrosis development	32:61	pulmonary fibrosis development	32:61	Qi-Long-Tian capsule alleviates pulmonary fibrosis development by modulating inflammatory response and gut microbiota.
36810971	1	148	theme	Pulmonary	119:127	arg1	disease					201:207	a chronic, progressive, and fibrotic interstitial lung disease	146:207	a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate	146:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	1	148	theme	Pulmonary	119:127	arg1	PF					139:140	PF	139:140	PF	139:140	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	1	148	theme	Pulmonary	119:127	arg1	fibrosis					129:136	Pulmonary fibrosis	119:136	Pulmonary fibrosis (PF)	119:141	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	7	149	theme	factors	1432:1438	arg1	expressions					1400:1410	the mRNA and protein expressions	1379:1410	expressions	1400:1410	qRT-PCR and ELISA were used to detect the mRNA and protein expressions of pro-inflammatory factors include interleukin 1β (IL-1β), interleukin 6 (IL-6), transforming growth factor β1 (TGF-β1), tumor necrosis factor α (TNF-α) in lung tissues and serums, and the inflammation-mediating factors include tight junction protein (ZO-1, Claudin, Occludin).
36810971	2	150	theme	Long	388:391	arg1	aspergillum					404:414	Long [Pheretima aspergillum	388:414	Di Long [Pheretima aspergillum (E. Perrier)	385:427	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	15	151	theme	intestinal	3188:3197	arg1	barrier					3207:3213	the intestinal mucosal barrier	3184:3213	the intestinal mucosal barrier	3184:3213	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	9	152	theme	flora	1955:1959	arg1	diversity					1931:1939	diversity	1931:1939	diversity	1931:1939	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	9	152	theme	flora	1955:1959	arg1	abundance					1917:1925	abundance	1917:1925	abundance	1917:1925	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	2	153	dep	Radix	471:475	arg1	Rhizoma					480:486	Rhodiolae Crenulatae Radix et Rhizoma	450:486	Rhodiolae Crenulatae Radix et Rhizoma	450:486	Qi-Long-Tian (QLT) capsule is an herbal formula with great potential for antifibrotic effects, consisting of San Qi (Notoginseng Radix et Rhizoma), Di Long [Pheretima aspergillum (E. Perrier)], and Hong Jingtian (Rhodiolae Crenulatae Radix et Rhizoma), and has been used in clinical practice for many years.
36810971	8	154	theme	fatty	1790:1794	arg1	SCFAs					1803:1807	SCFAs	1803:1807	SCFAs	1803:1807	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	8	154	theme	fatty	1790:1794	arg1	acids					1796:1800	short-chain fatty acids	1778:1800	short-chain fatty acids (SCFAs)	1778:1808	ELISA was used to detect the protein expressions of secretory immunoglobulin A (sIgA), short-chain fatty acids (SCFAs), and lipopolysaccharide (LPS) in colonic tissues.
36810971	10	155	theme	QLT	2089:2091	arg1	capsule					2093:2099	QLT capsule	2089:2099	QLT capsule	2089:2099	QLT capsule effectively improved the status of pulmonary fibrosis and reduced HYP.
36810971	4	156	theme	Thirty-six	731:740	arg1	mice					742:745	Thirty-six mice	731:745	Thirty-six mice	731:745	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	9	157	with	correlation	2050:2060	arg1	factors					2080:2086	inflammatory factors	2067:2086	inflammatory factors	2067:2086	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36810971	6	158	theme	main	1182:1185	arg1	indicators					1187:1196	the main indicators	1178:1196	the main indicators of PF	1178:1202	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	6	158	theme	main	1182:1185	arg1	changes					1167:1173	changes	1167:1173	changes	1167:1173	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	15	159	theme	inflammatory	3266:3277	arg1	secretion					3286:3294	inflammatory factor secretion	3266:3294	inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation	3266:3356	All these results suggest that QLT capsule intervenes in pulmonary fibrosis by regulating the differential genera of intestinal flora, increasing immunoglobulin secretion, repairing the intestinal mucosal barrier, reducing LPS entry into the blood, and decreasing inflammatory factor secretion in the serum, which in turn alleviates pulmonary inflammation.
36810971	4	160	theme	QLT	863:865	arg1	QM					894:895	QM	894:895	QM	894:895	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	160	theme	QLT	863:865	arg1	group					887:891	QLT capsule medium dose group	863:891	QLT capsule medium dose group (QM)	863:896	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	16	161	from	mechanism	3396:3404	arg1	PF					3424:3425	PF	3424:3425	PF	3424:3425	This study clarifies the therapeutic mechanism of QLT capsule in PF and provides a theoretical basis for it.
36810971	16	162	theme	therapeutic	3384:3394	arg1	mechanism					3396:3404	the therapeutic mechanism	3380:3404	the therapeutic mechanism of QLT capsule in PF	3380:3425	This study clarifies the therapeutic mechanism of QLT capsule in PF and provides a theoretical basis for it.
36810971	13	163	theme	relative	2696:2703	arg1	abundance					2705:2713	the relative abundance	2692:2713	the relative abundance of Bacteroidia (which might limit the onset of inflammation)	2692:2774	QLT capsule significantly increased the relative abundance of Bacteroidia (which might limit the onset of inflammation) and decreased the relative abundance of Clostridia (which might promote inflammation).
36810971	4	164	theme	capsule	903:909	arg1	QH					928:929	QH	928:929	QH	928:929	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	164	theme	capsule	903:909	arg1	group					921:925	QLT capsule high dose group	899:925	QLT capsule high dose group (QH)	899:930	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	16	165	theme	QLT	3409:3411	arg1	capsule					3413:3419	QLT capsule	3409:3419	QLT capsule in PF	3409:3425	This study clarifies the therapeutic mechanism of QLT capsule in PF and provides a theoretical basis for it.
36810971	6	166	theme	hydroxyproline	1241:1254	arg1	expression					1227:1236	the expression	1223:1236	the expression of hydroxyproline (HYP) related to collagen metabolism	1223:1291	HE staining and Masson's staining were used to detect changes as the main indicators of PF in each group, and the expression of hydroxyproline (HYP) related to collagen metabolism was detected by and alkaline hydrolysis method.
36810971	1	167	theme	mortality	221:229	arg1	rate					231:234	a high mortality rate	214:234	a high mortality rate	214:234	Pulmonary fibrosis (PF) is a chronic, progressive, and fibrotic interstitial lung disease with a high mortality rate.
36810971	4	168	theme	dose	916:919	arg1	QH					928:929	QH	928:929	QH	928:929	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	4	168	theme	dose	916:919	arg1	group					921:925	QLT capsule high dose group	899:925	QLT capsule high dose group (QH)	899:930	Thirty-six mice were randomly divided into 6 groups: control group (control), model group (model), QLT capsule low dose group (QL), QLT capsule medium dose group (QM), QLT capsule high dose group (QH), and pirfenidone group (PFD).
36810971	14	169	from	factors	2987:2993	arg1	PF					2998:2999	PF	2998:2999	PF	2998:2999	In addition, these two enterobacteria were closely associated with pro-inflammatory-related indicators and pro-inflammatory factors in PF.
36810971	3	170	theme	gut	621:623	arg1	microbiota					625:634	gut microbiota	621:634	gut microbiota	621:634	To explore the relationship between the effects of Qi-Long-Tian capsule and gut microbiota of PF mice, pulmonary fibrosis model were established by tracheal drip injection of bleomycin.
36810971	9	171	theme	QM	1988:1989	arg1	groups					1991:1996	the control, model, and QM groups	1964:1996	groups	1991:1996	16sRNA gene sequencing was used to detect changes in the abundance and diversity of intestinal flora in the control, model, and QM groups, to search for differential genera, and analyze the correlation with inflammatory factors.
36268867	10	0	theme	NEAU-yn17T	1335:1344	arg1	44231T					1390:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	2	1	theme	cellulose-degrading	155:173	arg1	actinobacterium					175:189	A novel cellulose-degrading actinobacterium	147:189	A novel cellulose-degrading actinobacterium	147:189	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	3	2	theme	Saccharothrix	545:557	arg1	SA152T					570:575	Saccharothrix saharensis SA152T	545:575	Saccharothrix saharensis SA152T (99.0 %)	545:584	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	2	theme	Saccharothrix	545:557	arg1	%					583:583	99.0 %	578:583	99.0 %	578:583	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	10	3	theme	SA152T	1361:1366	arg1	44231T					1390:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	2	4	theme	Autonomous	283:292	arg1	Region					294:299	Inner Mongolia Autonomous Region	268:299	Inner Mongolia Autonomous Region	268:299	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	2	4	theme	Autonomous	283:292	arg1	Chifeng					259:265	Chifeng	259:265	Chifeng	259:265	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	6	5	theme	polar	879:883	arg1	lipids					885:890	The polar lipids	875:890	The polar lipids	875:890	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	3	6	dep	Saccharothrix	587:599	arg1	texasensis					601:610	texasensis	601:610	texasensis	601:610	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	13	7	theme	protein-coding	1802:1815	arg1	genes					1817:1821	8 994 protein-coding genes	1796:1821	8 994 protein-coding genes	1796:1821	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	11	8	theme	reference	1627:1635	arg1	strains					1637:1643	its reference strains	1623:1643	its reference strains	1623:1643	soap8 % (36.3-41.3 %) and 44.8 % (42.2-47.3 %) and the ANI values between them were determined to be 90.2, 89.8 and 91.7 %, the results indicated that strain NEAU-S10T could be distinguished from its reference strains.
36268867	6	9	contain	contained	892:900	arg2	diphosphatidylglycerol					902:923	diphosphatidylglycerol	902:923	diphosphatidylglycerol	902:923	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	6	9	contain	contained	892:900	arg2	phosphatidylinositol					988:1007	phosphatidylinositol	988:1007	phosphatidylinositol	988:1007	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	6	9	contain	contained	892:900	arg2	phosphatidylmonomethylethanolamine					926:959	phosphatidylmonomethylethanolamine	926:959	phosphatidylmonomethylethanolamine	926:959	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	6	9	contain	contained	892:900	arg1	lipids					885:890	The polar lipids	875:890	The polar lipids	875:890	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	6	9	contain	contained	892:900	arg2	phosphatidylethanolamine					962:985	phosphatidylethanolamine	962:985	phosphatidylethanolamine	962:985	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	6	9	contain	contained	892:900	arg2	mannoside					1034:1042	phosphatidylinositol mannoside	1013:1042	phosphatidylinositol mannoside	1013:1042	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	3	10	theme	Saccharothrix	587:599	arg1	44231T					616:621	Saccharothrix texasensis DSM 44231T	587:621	Saccharothrix texasensis DSM 44231T (98.5 %)	587:630	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	10	theme	Saccharothrix	587:599	arg1	%					629:629	98.5 %	624:629	98.5 %	624:629	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	4	11	theme	Physiological	687:699	arg1	characteristics					720:734	Physiological and chemotaxonomic characteristics	687:734	Physiological and chemotaxonomic characteristics of the strain	687:748	Physiological and chemotaxonomic characteristics of the strain further supported its affiliation to the genus Saccharothrix.
36268867	15	12	theme	Saccharothrix	2081:2093	arg1	sp					2103:2104	the name Saccharothrix luteola sp	2072:2104	the name Saccharothrix luteola sp	2072:2104	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	4	13	theme	chemotaxonomic	705:718	arg1	characteristics					720:734	Physiological and chemotaxonomic characteristics	687:734	Physiological and chemotaxonomic characteristics of the strain	687:748	Physiological and chemotaxonomic characteristics of the strain further supported its affiliation to the genus Saccharothrix.
36268867	13	14	theme	Annotation	1760:1769	arg1	PGAP					1781:1784	PGAP	1781:1784	PGAP	1781:1784	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	13	14	theme	Annotation	1760:1769	arg1	Pipeline					1771:1778	The NCBI Prokaryotic Genome Annotation Pipeline	1732:1778	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP)	1732:1785	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	2	15	theme	Inner	268:272	arg1	Region					294:299	Inner Mongolia Autonomous Region	268:299	Inner Mongolia Autonomous Region	268:299	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	2	15	theme	Inner	268:272	arg1	Chifeng					259:265	Chifeng	259:265	Chifeng	259:265	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	1	16	theme	Saccharothrix	132:144	arg1	soil					90:93	soil	90:93	soil	90:93	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	1	16	theme	Saccharothrix	132:144	arg1	description					107:117	emended description	99:117	emended description of the genus Saccharothrix	99:144	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	16	17	theme	=CCTCC AA	2141:2149	arg1	NEAU-S10T					2130:2138	NEAU-S10T	2130:2138	NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain	2130:2189	nov. is proposed, with NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain.
36268867	16	17	theme	=CCTCC AA	2141:2149	arg1	34800T					2164:2169	=CCTCC AA 2020037T=JCM 34800T	2141:2169	=CCTCC AA 2020037T=JCM 34800T	2141:2169	nov. is proposed, with NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain.
36268867	9	18	theme	genomic	1222:1228	arg1	content					1238:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content was 71.8 mol%.
36268867	9	18	theme	genomic	1222:1228	arg1	%					1258:1258	71.8 mol%	1250:1258	71.8 mol%	1250:1258	The genomic DNA G+C content was 71.8 mol%.
36268867	3	19	theme	DSM	612:614	arg1	44231T					616:621	Saccharothrix texasensis DSM 44231T	587:621	Saccharothrix texasensis DSM 44231T (98.5 %)	587:630	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	19	theme	DSM	612:614	arg1	%					629:629	98.5 %	624:629	98.5 %	624:629	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	10	20	theme	DSM	1386:1388	arg1	44231T					1390:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	9	21	theme	G+C	1234:1236	arg1	content					1238:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content was 71.8 mol%.
36268867	9	21	theme	G+C	1234:1236	arg1	%					1258:1258	71.8 mol%	1250:1258	71.8 mol%	1250:1258	The genomic DNA G+C content was 71.8 mol%.
36268867	15	22	theme	Saccharothrix	2047:2059	arg1	species					2026:2032	a novel species	2018:2032	a novel species	2018:2032	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	13	23	theme	Prokaryotic	1741:1751	arg1	PGAP					1781:1784	PGAP	1781:1784	PGAP	1781:1784	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	13	23	theme	Prokaryotic	1741:1751	arg1	Pipeline					1771:1778	The NCBI Prokaryotic Genome Annotation Pipeline	1732:1778	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP)	1732:1785	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	8	24	dep	iso-C16 	1150:1157	arg1	 0					1214:1215	 0	1214:1215	 0	1214:1215	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	8	24	dep	iso-C16 	1150:1157	arg1	iso-C17 					1205:1212	iso-C17 	1205:1212	iso-C17 	1205:1212	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	8	24	dep	iso-C16 	1150:1157	arg1	 0					1198:1199	 0	1198:1199	 0	1198:1199	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	8	24	dep	iso-C16 	1150:1157	arg1	 0					1168:1169	 0	1168:1169	 0	1168:1169	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	8	24	dep	iso-C16 	1150:1157	arg1	C16 					1163:1166	C16 	1163:1166	C16 	1163:1166	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	14	25	theme	staining	1855:1862	arg1	test					1864:1867	Congo red staining test	1845:1867	Congo red staining test	1845:1867	Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose.
36268867	2	26	attach	isolated	225:232	arg2	actinobacterium					175:189	A novel cellulose-degrading actinobacterium	147:189	A novel cellulose-degrading actinobacterium	147:189	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	2	26	attach	isolated	225:232	arg1	soil					239:242	soil	239:242	soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China,	239:310	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	3	27	theme	rRNA	390:393	arg1	sequence					400:407	the 16S rRNA gene sequence	382:407	the 16S rRNA gene sequence	382:407	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	0	28	theme	Saccharothrix	0:12	arg1	sp					22:23	Saccharothrix luteola sp	0:23	Saccharothrix luteola sp.	0:24	Saccharothrix luteola sp.
36268867	5	29	contain	contained	834:842	arg2	mannose					866:872	mannose	866:872	mannose	866:872	The whole-cell sugars contained galactose, ribose and mannose.
36268867	5	29	contain	contained	834:842	arg2	ribose					855:860	ribose	855:860	ribose	855:860	The whole-cell sugars contained galactose, ribose and mannose.
36268867	5	29	contain	contained	834:842	arg2	galactose					844:852	galactose	844:852	galactose	844:852	The whole-cell sugars contained galactose, ribose and mannose.
36268867	5	29	contain	contained	834:842	arg1	sugars					827:832	The whole-cell sugars	812:832	The whole-cell sugars	812:832	The whole-cell sugars contained galactose, ribose and mannose.
36268867	4	30	theme	strain	743:748	arg1	characteristics					720:734	Physiological and chemotaxonomic characteristics	687:734	Physiological and chemotaxonomic characteristics of the strain	687:748	Physiological and chemotaxonomic characteristics of the strain further supported its affiliation to the genus Saccharothrix.
36268867	12	31	theme	genome	1660:1665	arg1	sequence					1667:1674	The assembled genome sequence	1646:1674	The assembled genome sequence of strain NEAU-S10T	1646:1694	The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long.
36268867	12	31	theme	genome	1660:1665	arg1	long					1726:1729	long	1726:1729	long	1726:1729	The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long.
36268867	3	32	theme	sequence	400:407	arg1	similarity					368:377	Pairwise similarity	359:377	Pairwise similarity of the 16S rRNA gene sequence	359:407	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	15	33	theme	luteola	2095:2101	arg1	sp					2103:2104	the name Saccharothrix luteola sp	2072:2104	the name Saccharothrix luteola sp	2072:2104	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	12	34	theme	strain	1679:1684	arg1	NEAU-S10T					1686:1694	strain NEAU-S10T	1679:1694	strain NEAU-S10T	1679:1694	The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long.
36268867	8	35	dep	 0	1168:1169	arg1	 0					1185:1186	 0	1185:1186	 0	1185:1186	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	8	36	theme	fatty	1133:1137	arg1	iso-C16 					1150:1157	iso-C16 	1150:1157	iso-C16 	1150:1157	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	8	36	theme	fatty	1133:1137	arg1	acids					1139:1143	The major fatty acids	1123:1143	The major fatty acids	1123:1143	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	1	37	theme	novel	34:38	arg1	actinobacterium					60:74	a novel cellulose-degrading actinobacterium	32:74	a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix	32:144	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	1	37	theme	novel	34:38	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	10	38	theme	DNA-DNA	1283:1289	arg1	hybridization					1291:1303	digital DNA-DNA hybridization	1275:1303	digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1275:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	3	39	theme	carnea	517:522	arg1	NEAU-yn17T					524:533	Saccharothrix carnea NEAU-yn17T	503:533	Saccharothrix carnea NEAU-yn17T (99.2 %)	503:542	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	39	theme	carnea	517:522	arg1	%					541:541	99.2 %	536:541	99.2 %	536:541	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	40	dep	Saccharothrix	636:648	arg1	xinjiangensis					650:662	xinjiangensis	650:662	xinjiangensis	650:662	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	16	41	theme	type	2179:2182	arg1	strain					2184:2189	the type strain	2175:2189	the type strain	2175:2189	nov. is proposed, with NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain.
36268867	2	42	theme	PR	302:303	arg1	Chifeng					259:265	Chifeng	259:265	Chifeng	259:265	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	2	42	theme	PR	302:303	arg1	China					305:309	PR China	302:309	PR China	302:309	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	2	43	theme	novel	149:153	arg1	actinobacterium					175:189	A novel cellulose-degrading actinobacterium	147:189	A novel cellulose-degrading actinobacterium	147:189	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	15	44	theme	novel	2020:2024	arg1	species					2026:2032	a novel species	2018:2032	a novel species	2018:2032	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	3	45	theme	saharensis	559:568	arg1	SA152T					570:575	Saccharothrix saharensis SA152T	545:575	Saccharothrix saharensis SA152T (99.0 %)	545:584	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	45	theme	saharensis	559:568	arg1	%					583:583	99.0 %	578:583	99.0 %	578:583	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	1	46	attach	isolated	76:83	arg2	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	1	46	attach	isolated	76:83	arg1	soil					90:93	soil	90:93	soil	90:93	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	1	46	attach	isolated	76:83	arg2	actinobacterium					60:74	a novel cellulose-degrading actinobacterium	32:74	a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix	32:144	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	1	46	attach	isolated	76:83	arg1	description					107:117	emended description	99:117	emended description of the genus Saccharothrix	99:144	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	15	47	theme	strain	1990:1995	arg1	NEAU-S10T					1997:2005	strain NEAU-S10T	1990:2005	strain NEAU-S10T	1990:2005	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	6	48	theme	phosphatidylinositol	1013:1032	arg1	mannoside					1034:1042	phosphatidylinositol mannoside	1013:1042	phosphatidylinositol mannoside	1013:1042	The polar lipids contained diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
36268867	10	49	theme	38	1424:1425	arg1	%					1420:1420	37.6-42.6 %	1410:1420	37.6-42.6 %	1410:1420	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	10	49	theme	38	1424:1425	arg1	levels					1265:1270	The levels	1261:1270	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1261:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	10	49	theme	38	1424:1425	arg1	%					1407:1407	40.1 %	1402:1407	40.1 % (37.6-42.6 %)	1402:1421	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	15	50	theme	name	2076:2079	arg1	sp					2103:2104	the name Saccharothrix luteola sp	2072:2104	the name Saccharothrix luteola sp	2072:2104	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	13	51	theme	Genome	1753:1758	arg1	PGAP					1781:1784	PGAP	1781:1784	PGAP	1781:1784	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	13	51	theme	Genome	1753:1758	arg1	Pipeline					1771:1778	The NCBI Prokaryotic Genome Annotation Pipeline	1732:1778	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP)	1732:1785	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	2	52	theme	Mongolia	274:281	arg1	Region					294:299	Inner Mongolia Autonomous Region	268:299	Inner Mongolia Autonomous Region	268:299	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	2	52	theme	Mongolia	274:281	arg1	Chifeng					259:265	Chifeng	259:265	Chifeng	259:265	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	1	53	theme	genus	126:130	arg1	Saccharothrix					132:144	the genus Saccharothrix	122:144	the genus Saccharothrix	122:144	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	16	54	theme	2020037T=JCM	2151:2162	arg1	NEAU-S10T					2130:2138	NEAU-S10T	2130:2138	NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain	2130:2189	nov. is proposed, with NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain.
36268867	16	54	theme	2020037T=JCM	2151:2162	arg1	34800T					2164:2169	=CCTCC AA 2020037T=JCM 34800T	2141:2169	=CCTCC AA 2020037T=JCM 34800T	2141:2169	nov. is proposed, with NEAU-S10T (=CCTCC AA 2020037T=JCM 34800T) as the type strain.
36268867	2	55	theme	polyphasic	338:347	arg1	approach					349:356	a polyphasic approach	336:356	a polyphasic approach	336:356	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	14	56	contain	had	1901:1903	arg1	NEAU-S10T					1891:1899	strain NEAU-S10T	1884:1899	strain NEAU-S10T	1884:1899	Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose.
36268867	14	56	contain	had	1901:1903	arg2	potential					1909:1917	the potential to degrade cellulose	1905:1938	the potential to degrade cellulose	1905:1938	Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose.
36268867	11	57	theme	strain	1578:1583	arg1	NEAU-S10T					1585:1593	strain NEAU-S10T	1578:1593	strain NEAU-S10T	1578:1593	soap8 % (36.3-41.3 %) and 44.8 % (42.2-47.3 %) and the ANI values between them were determined to be 90.2, 89.8 and 91.7 %, the results indicated that strain NEAU-S10T could be distinguished from its reference strains.
36268867	9	58	theme	DNA	1230:1232	arg1	content					1238:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content was 71.8 mol%.
36268867	9	58	theme	DNA	1230:1232	arg1	%					1258:1258	71.8 mol%	1250:1258	71.8 mol%	1250:1258	The genomic DNA G+C content was 71.8 mol%.
36268867	15	59	theme	genus	2041:2045	arg1	Saccharothrix					2047:2059	the genus Saccharothrix	2037:2059	the genus Saccharothrix	2037:2059	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	10	60	theme	texasensis	1375:1384	arg1	44231T					1390:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1325:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	13	61	theme	NCBI	1736:1739	arg1	PGAP					1781:1784	PGAP	1781:1784	PGAP	1781:1784	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	13	61	theme	NCBI	1736:1739	arg1	Pipeline					1771:1778	The NCBI Prokaryotic Genome Annotation Pipeline	1732:1778	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP)	1732:1785	The NCBI Prokaryotic Genome Annotation Pipeline (PGAP) revealed 8 994 protein-coding genes.
36268867	7	62	theme	predominant	1049:1059	arg1	MK-9					1079:1082	MK-9	1079:1082	MK-9	1079:1082	The predominant menaquinones were MK-9(H0), MK-9(H2), MK-9(H4) and MK-10(H4).
36268867	7	62	theme	predominant	1049:1059	arg1	menaquinones					1061:1072	The predominant menaquinones	1045:1072	The predominant menaquinones	1045:1072	The predominant menaquinones were MK-9(H0), MK-9(H2), MK-9(H4) and MK-10(H4).
36268867	1	63	theme	cellulose-degrading	40:58	arg1	actinobacterium					60:74	a novel cellulose-degrading actinobacterium	32:74	a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix	32:144	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	1	63	theme	cellulose-degrading	40:58	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	9	64	theme	71.8 mol	1250:1257	arg1	content					1238:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content	1218:1244	The genomic DNA G+C content was 71.8 mol%.
36268867	9	64	theme	71.8 mol	1250:1257	arg1	%					1258:1258	71.8 mol%	1250:1258	71.8 mol%	1250:1258	The genomic DNA G+C content was 71.8 mol%.
36268867	3	65	theme	Pairwise	359:366	arg1	similarity					368:377	Pairwise similarity	359:377	Pairwise similarity of the 16S rRNA gene sequence	359:407	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	0	66	theme	luteola	14:20	arg1	sp					22:23	Saccharothrix luteola sp	0:23	Saccharothrix luteola sp.	0:24	Saccharothrix luteola sp.
36268867	14	67	theme	red	1851:1853	arg1	test					1864:1867	Congo red staining test	1845:1867	Congo red staining test	1845:1867	Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose.
36268867	3	68	theme	gene	395:398	arg1	sequence					400:407	the 16S rRNA gene sequence	382:407	the 16S rRNA gene sequence	382:407	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	5	69	theme	whole-cell	816:825	arg1	sugars					827:832	The whole-cell sugars	812:832	The whole-cell sugars	812:832	The whole-cell sugars contained galactose, ribose and mannose.
36268867	1	70	theme	emended	99:105	arg1	description					107:117	emended description	99:117	emended description of the genus Saccharothrix	99:144	nov., a novel cellulose-degrading actinobacterium isolated from soil and emended description of the genus Saccharothrix.
36268867	3	71	theme	16S	386:388	arg1	rRNA					390:393	the 16S rRNA	382:393	the 16S rRNA gene sequence	382:407	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	2	72	theme	strain	203:208	arg1	NEAU-S10T					210:218	strain NEAU-S10T	203:218	strain NEAU-S10T	203:218	A novel cellulose-degrading actinobacterium, designated strain NEAU-S10T, was isolated from soil collected from Chifeng, Inner Mongolia Autonomous Region, PR China, and characterized using a polyphasic approach.
36268867	14	73	theme	Genomic	1824:1830	arg1	analysis					1832:1839	Genomic analysis	1824:1839	Genomic analysis	1824:1839	Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose.
36268867	3	74	theme	strain	421:426	arg1	NEAU-S10T					428:436	strain NEAU-S10T	421:436	strain NEAU-S10T	421:436	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	74	theme	strain	421:426	arg1	representative					444:457	a representative	442:457	a representative of Saccharothrix	442:474	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	10	75	theme	digital	1275:1281	arg1	hybridization					1291:1303	digital DNA-DNA hybridization	1275:1303	digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1275:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	3	76	theme	Saccharothrix	462:474	arg1	NEAU-S10T					428:436	strain NEAU-S10T	421:436	strain NEAU-S10T	421:436	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	76	theme	Saccharothrix	462:474	arg1	representative					444:457	a representative	442:457	a representative of Saccharothrix	442:474	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	12	77	theme	NEAU-S10T	1686:1694	arg1	sequence					1667:1674	The assembled genome sequence	1646:1674	The assembled genome sequence of strain NEAU-S10T	1646:1694	The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long.
36268867	12	77	theme	NEAU-S10T	1686:1694	arg1	long					1726:1729	long	1726:1729	long	1726:1729	The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long.
36268867	15	78	theme	phenotypic	1957:1966	arg1	results					1968:1974	The genomic and phenotypic results	1941:1974	The genomic and phenotypic results	1941:1974	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	12	79	theme	assembled	1650:1658	arg1	sequence					1667:1674	The assembled genome sequence	1646:1674	The assembled genome sequence of strain NEAU-S10T	1646:1694	The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long.
36268867	12	79	theme	assembled	1650:1658	arg1	long					1726:1729	long	1726:1729	long	1726:1729	The assembled genome sequence of strain NEAU-S10T was found to be 10 305 394 bp long.
36268867	3	80	theme	NBRC	664:667	arg1	%					683:683	98.5 %	678:683	98.5 %	678:683	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	80	theme	NBRC	664:667	arg1	101911T					669:675	Saccharothrix xinjiangensis NBRC 101911T	636:675	Saccharothrix xinjiangensis NBRC 101911T (98.5 %)	636:684	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	81	theme	Saccharothrix	503:515	arg1	NEAU-yn17T					524:533	Saccharothrix carnea NEAU-yn17T	503:533	Saccharothrix carnea NEAU-yn17T (99.2 %)	503:542	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	81	theme	Saccharothrix	503:515	arg1	%					541:541	99.2 %	536:541	99.2 %	536:541	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	8	82	theme	major	1127:1131	arg1	iso-C16 					1150:1157	iso-C16 	1150:1157	iso-C16 	1150:1157	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	8	82	theme	major	1127:1131	arg1	acids					1139:1143	The major fatty acids	1123:1143	The major fatty acids	1123:1143	The major fatty acids were iso-C16 : 0, C16 : 0, anteiso-C17 : 0, iso-C15 : 0 and iso-C17 : 0.
36268867	15	83	theme	genomic	1945:1951	arg1	results					1968:1974	The genomic and phenotypic results	1941:1974	The genomic and phenotypic results	1941:1974	The genomic and phenotypic results indicate that strain NEAU-S10T represents a novel species of the genus Saccharothrix, for which the name Saccharothrix luteola sp.
36268867	14	84	theme	strain	1884:1889	arg1	NEAU-S10T					1891:1899	strain NEAU-S10T	1884:1899	strain NEAU-S10T	1884:1899	Genomic analysis and Congo red staining test indicated that strain NEAU-S10T had the potential to degrade cellulose.
36268867	3	85	theme	Saccharothrix	636:648	arg1	%					683:683	98.5 %	678:683	98.5 %	678:683	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	3	85	theme	Saccharothrix	636:648	arg1	101911T					669:675	Saccharothrix xinjiangensis NBRC 101911T	636:675	Saccharothrix xinjiangensis NBRC 101911T (98.5 %)	636:684	Pairwise similarity of the 16S rRNA gene sequence showed that strain NEAU-S10T was a representative of Saccharothrix and was closely related to Saccharothrix carnea NEAU-yn17T (99.2 %), Saccharothrix saharensis SA152T (99.0 %), Saccharothrix texasensis DSM 44231T (98.5 %) and Saccharothrix xinjiangensis NBRC 101911T (98.5 %).
36268867	11	86	theme	ANI	1482:1484	arg1	%					1548:1548	90.2, 89.8 and 91.7 %	1528:1548	90.2, 89.8 and 91.7 %	1528:1548	soap8 % (36.3-41.3 %) and 44.8 % (42.2-47.3 %) and the ANI values between them were determined to be 90.2, 89.8 and 91.7 %, the results indicated that strain NEAU-S10T could be distinguished from its reference strains.
36268867	11	86	theme	ANI	1482:1484	arg1	values					1486:1491	the ANI values	1478:1491	the ANI values between them	1478:1504	soap8 % (36.3-41.3 %) and 44.8 % (42.2-47.3 %) and the ANI values between them were determined to be 90.2, 89.8 and 91.7 %, the results indicated that strain NEAU-S10T could be distinguished from its reference strains.
36268867	4	87	theme	genus	791:795	arg1	Saccharothrix					797:809	the genus Saccharothrix	787:809	the genus Saccharothrix	787:809	Physiological and chemotaxonomic characteristics of the strain further supported its affiliation to the genus Saccharothrix.
36268867	10	88	theme	hybridization	1291:1303	arg1	levels					1265:1270	The levels	1261:1270	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T	1261:1395	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
36268867	10	88	theme	hybridization	1291:1303	arg1	%					1407:1407	40.1 %	1402:1407	40.1 % (37.6-42.6 %)	1402:1421	The levels of digital DNA-DNA hybridization between isolate and S. carnea NEAU-yn17T, S. saharensis SA152T and S. texasensis DSM 44231T were 40.1 % (37.6-42.6 %), 38.
35630600	13	0	theme	antimicrobial	2308:2320	arg1	effects					2340:2346	antimicrobial and antigenotoxic effects	2308:2346	antimicrobial and antigenotoxic effects	2308:2346	Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.
35630600	7	1	theme	mm	1355:1356	arg1	zones					1330:1334	inhibition zones	1319:1334	inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively	1319:1384	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	7	2	theme	inhibition	1319:1328	arg1	zones					1330:1334	inhibition zones	1319:1334	inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively	1319:1384	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	10	3	theme	rRNA	1731:1734	arg1	analysis					1750:1757	The 16S rRNA gene sequence analysis	1723:1757	The 16S rRNA gene sequence analysis	1723:1757	The 16S rRNA gene sequence analysis showed that the potential strains belonged to the genus Streptomyces.
35630600	6	4	theme	pathogenic	1141:1150	arg1	strains					1162:1168	six pathogenic microbial strains	1137:1168	six pathogenic microbial strains	1137:1168	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	9	5	theme	other	1520:1524	arg1	hand					1526:1529	the other hand	1516:1529	the other hand	1516:1529	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	12	6	theme	dose-dependent	2252:2265	arg1	manner					2267:2272	a dose-dependent manner	2250:2272	a dose-dependent manner	2250:2272	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	13	7	theme	C.	2285:2286	arg1	extract					2296:2302	C. procera extract	2285:2302	C. procera extract	2285:2302	Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.
35630600	3	8	theme	Soil-extract	535:546	arg1	medium					548:553	Soil-extract medium	535:553	Soil-extract medium supplemented with glycerol as a carbon source	535:599	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	11	9	theme	procera	1846:1852	arg1	extract					1854:1860	C. procera extract	1843:1860	C. procera extract	1843:1860	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	12	10	theme	DNA	2229:2231	arg1	fragmentation					2233:2245	DNA fragmentation	2229:2245	chromosomal abnormalities as well as DNA fragmentation	2192:2245	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	10	11	theme	sequence	1741:1748	arg1	analysis					1750:1757	The 16S rRNA gene sequence analysis	1723:1757	The 16S rRNA gene sequence analysis	1723:1757	The 16S rRNA gene sequence analysis showed that the potential strains belonged to the genus Streptomyces.
35630600	9	12	dep	aureus	1614:1619	arg1	MIC					1622:1624	MIC	1622:1624	MIC of 86 µg/mL	1622:1636	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	1	13	theme	wild	190:193	arg1	procera					164:170	Calotropis procera	153:170	Calotropis procera (C. procera)	153:183	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	13	theme	wild	190:193	arg1	plant					221:225	a medicinal plant	209:225	a medicinal plant found in abundance throughout Saudi Arabia	209:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	13	theme	wild	190:193	arg1	shrub					195:199	a wild shrub	188:199	a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia	188:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	5	14	theme	gombasterol	961:971	arg1	%					980:980	2.14%	976:980	2.14%	976:980	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	5	14	theme	gombasterol	961:971	arg1	A					973:973	gombasterol A	961:973	gombasterol A (2.14%)	961:981	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	3	15	theme	starch-casein	605:617	arg1	medium					624:629	starch-casein agar medium	605:629	starch-casein agar medium	605:629	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	6	16	theme	inhibitory	1098:1107	arg1	MIC					1124:1126	MIC	1124:1126	MIC	1124:1126	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	6	16	theme	inhibitory	1098:1107	arg1	concentration					1109:1121	minimum inhibitory concentration	1090:1121	minimum inhibitory concentration (MIC)	1090:1127	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	4	17	theme	compounds	720:728	arg1	total					708:712	a total	706:712	a total of 31 compounds	706:728	From the plant, a total of 31 compounds were identified using gas chromatography/mass spectrometry (GC-MS).
35630600	4	18	theme	gas	752:754	arg1	spectrometry					776:787	gas chromatography/mass spectrometry	752:787	gas chromatography/mass spectrometry (GC-MS)	752:795	From the plant, a total of 31 compounds were identified using gas chromatography/mass spectrometry (GC-MS).
35630600	4	18	theme	gas	752:754	arg1	GC-MS					790:794	GC-MS	790:794	GC-MS	790:794	From the plant, a total of 31 compounds were identified using gas chromatography/mass spectrometry (GC-MS).
35630600	10	19	theme	genus	1809:1813	arg1	Streptomyces					1815:1826	the genus Streptomyces	1805:1826	the genus Streptomyces	1805:1826	The 16S rRNA gene sequence analysis showed that the potential strains belonged to the genus Streptomyces.
35630600	8	20	with	inhibitor	1437:1445	arg1	MIC					1479:1481	MIC	1479:1481	MIC ranging from 0.60-1.50 mg/mL	1479:1510	The plant extract was considered to be a moderate inhibitor against Bacillus subtilis, with MIC ranging from 0.60-1.50 mg/mL.
35630600	2	21	theme	actinobacteria	477:490	arg1	effects					492:498	its rhizospheric actinobacteria effects	460:498	its rhizospheric actinobacteria effects against pathogenic microorganisms	460:532	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	6	22	theme	well-diffusion	1065:1078	arg1	assay					1080:1084	an agar well-diffusion assay	1057:1084	an agar well-diffusion assay	1057:1084	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	7	23	with	active	1232:1237	arg1	zones					1330:1334	inhibition zones	1319:1334	inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively	1319:1384	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	1	24	theme	Calotropis	153:162	arg1	shrub					195:199	a wild shrub	188:199	a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia	188:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	24	theme	Calotropis	153:162	arg1	plant					221:225	a medicinal plant	209:225	a medicinal plant found in abundance throughout Saudi Arabia	209:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	24	theme	Calotropis	153:162	arg1	procera					164:170	Calotropis procera	153:170	Calotropis procera (C. procera)	153:183	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	3	25	used	used	635:638	arg2	medium					624:629	starch-casein agar medium	605:629	starch-casein agar medium	605:629	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	3	25	used	used	635:638	arg2	medium					548:553	Soil-extract medium	535:553	Soil-extract medium supplemented with glycerol as a carbon source	535:599	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	2	26	dep	activity	434:441	arg1	addition					404:411	addition	404:411	addition	404:411	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	13	27	theme	antigenotoxic	2326:2338	arg1	effects					2340:2346	antimicrobial and antigenotoxic effects	2308:2346	antimicrobial and antigenotoxic effects	2308:2346	Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.
35630600	0	28	theme	Actinobacteria	107:120	arg1	Antigenotoxicity					15:30	Antigenotoxicity	15:30	Antigenotoxicity	15:30	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	28	theme	Actinobacteria	107:120	arg1	Characterization					37:52	Characterization	37:52	Characterization	37:52	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	28	theme	Actinobacteria	107:120	arg1	Antimicrobial					0:12	Antimicrobial	0:12	Antimicrobial	0:12	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	28	theme	Actinobacteria	107:120	arg1	Studies					144:150	In Vitro and In Vivo Studies	123:150	In Vitro and In Vivo Studies	123:150	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	29	dep	In	123:124	arg1	Vitro					126:130	Vitro	126:130	Vitro	126:130	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	12	30	theme	oral	2050:2053	arg1	pretreatment					2055:2066	oral pretreatment	2050:2066	oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals	2050:2168	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	0	31	theme	In	123:124	arg1	Antigenotoxicity					15:30	Antigenotoxicity	15:30	Antigenotoxicity	15:30	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	31	theme	In	123:124	arg1	Characterization					37:52	Characterization	37:52	Characterization	37:52	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	31	theme	In	123:124	arg1	Antimicrobial					0:12	Antimicrobial	0:12	Antimicrobial	0:12	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	31	theme	In	123:124	arg1	Studies					144:150	In Vitro and In Vivo Studies	123:150	In Vitro and In Vivo Studies	123:150	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	2	32	theme	plant	450:454	arg1	effects					492:498	its rhizospheric actinobacteria effects	460:498	its rhizospheric actinobacteria effects against pathogenic microorganisms	460:532	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	2	32	theme	plant	450:454	arg1	activity					434:441	the antimicrobial activity	416:441	the antimicrobial activity of the plant	416:454	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	11	33	theme	mouse	1968:1972	arg1	cells					1982:1986	mouse somatic cells	1968:1986	mouse somatic cells	1968:1986	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	8	34	theme	plant	1391:1395	arg1	inhibitor					1437:1445	a moderate inhibitor	1426:1445	a moderate inhibitor against Bacillus subtilis	1426:1471	The plant extract was considered to be a moderate inhibitor against Bacillus subtilis, with MIC ranging from 0.60-1.50 mg/mL.
35630600	8	34	theme	plant	1391:1395	arg1	extract					1397:1403	The plant extract	1387:1403	The plant extract	1387:1403	The plant extract was considered to be a moderate inhibitor against Bacillus subtilis, with MIC ranging from 0.60-1.50 mg/mL.
35630600	1	35	dep	procera	164:170	arg1	procera					176:182	C. procera	173:182	C. procera	173:182	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	2	36	theme	ethanolic	368:376	arg1	extract					378:384	the ethanolic extract	364:384	the ethanolic extract of C. procera	364:398	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	5	37	theme	hexadecanoic	884:895	arg1	acid					897:900	hexadecanoic acid	884:900	hexadecanoic acid (5.55%)	884:908	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	5	37	theme	hexadecanoic	884:895	arg1	%					907:907	5.55%	903:907	5.55%	903:907	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	7	38	theme	procera	1195:1201	arg1	extract					1181:1187	The plant extract	1171:1187	The plant extract of C. procera	1171:1201	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	2	39	dep	composition	320:330	arg1	the					302:304	the	302:304	the	302:304	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	11	40	theme	DNA	1992:1994	arg1	assays					2010:2015	DNA fragmentation assays	1992:2015	DNA fragmentation assays	1992:2015	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	3	41	theme	actinobacteria	657:670	arg1	isolation					644:652	isolation	644:652	isolation of actinobacteria from rhizosphere	644:687	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	2	42	theme	procera	392:398	arg1	extract					378:384	the ethanolic extract	364:384	the ethanolic extract of C. procera	364:398	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	11	43	theme	fragmentation	1996:2008	arg1	assays					2010:2015	DNA fragmentation assays	1992:2015	DNA fragmentation assays	1992:2015	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	7	44	theme	plant	1175:1179	arg1	extract					1181:1187	The plant extract	1171:1187	The plant extract of C. procera	1171:1201	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	0	45	theme	procera	68:74	arg1	Antigenotoxicity					15:30	Antigenotoxicity	15:30	Antigenotoxicity	15:30	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	45	theme	procera	68:74	arg1	Characterization					37:52	Characterization	37:52	Characterization	37:52	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	45	theme	procera	68:74	arg1	Antimicrobial					0:12	Antimicrobial	0:12	Antimicrobial	0:12	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	45	theme	procera	68:74	arg1	Studies					144:150	In Vitro and In Vivo Studies	123:150	In Vitro and In Vivo Studies	123:150	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	5	46	theme	main	802:805	arg1	α-amyrin					823:830	α-amyrin	823:830	α-amyrin (39.36%)	823:839	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	5	46	theme	main	802:805	arg1	components					807:816	The main components	798:816	The main components	798:816	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	6	47	theme	procera	987:993	arg1	extract					1001:1007	C. procera plant extract	984:1007	C. procera plant extract's antimicrobial activity	984:1032	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	1	48	located	found	227:231	arg2	plant					221:225	a medicinal plant	209:225	a medicinal plant found in abundance throughout Saudi Arabia	209:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	48	located	found	227:231	arg2	shrub					195:199	a wild shrub	188:199	a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia	188:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	48	located	found	227:231	arg1	abundance					236:244	abundance	236:244	abundance	236:244	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	48	located	found	227:231	arg2	procera					164:170	Calotropis procera	153:170	Calotropis procera (C. procera)	153:183	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	49	theme	medicinal	211:219	arg1	procera					164:170	Calotropis procera	153:170	Calotropis procera (C. procera)	153:183	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	49	theme	medicinal	211:219	arg1	plant					221:225	a medicinal plant	209:225	a medicinal plant found in abundance throughout Saudi Arabia	209:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	1	49	theme	medicinal	211:219	arg1	shrub					195:199	a wild shrub	188:199	a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia	188:268	Calotropis procera (C. procera) is a wild shrub that is a medicinal plant found in abundance throughout Saudi Arabia.
35630600	13	50	theme	CP-induced	2356:2365	arg1	genotoxicity					2367:2378	CP-induced genotoxicity	2356:2378	CP-induced genotoxicity	2356:2378	Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.
35630600	9	51	theme	Candida	1687:1693	arg1	albicans					1695:1702	Candida albicans	1687:1702	Candida albicans (MIC of 35 µg/mL)	1687:1720	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	2	52	theme	antigenotoxic	336:348	arg1	properties					350:359	antigenotoxic properties	336:359	antigenotoxic properties	336:359	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	6	53	theme	microbial	1152:1160	arg1	strains					1162:1168	six pathogenic microbial strains	1137:1168	six pathogenic microbial strains	1137:1168	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	7	54	theme	mm	1345:1346	arg1	zones					1330:1334	inhibition zones	1319:1334	inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively	1319:1384	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	11	55	theme	-induced	1891:1898	arg1	genotoxicity					1900:1911	cyclophosphamide (CP)-induced genotoxicity	1870:1911	cyclophosphamide (CP)-induced genotoxicity	1870:1911	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	10	56	theme	16S	1727:1729	arg1	rRNA					1731:1734	16S rRNA	1727:1734	The 16S rRNA gene sequence analysis	1723:1757	The 16S rRNA gene sequence analysis showed that the potential strains belonged to the genus Streptomyces.
35630600	5	57	theme	lupeol	842:847	arg1	acetate					849:855	lupeol acetate	842:855	lupeol acetate (17.94%)	842:864	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	5	57	theme	lupeol	842:847	arg1	%					863:863	17.94%	858:863	17.94%	858:863	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	11	58	theme	chromosome	1940:1949	arg1	abnormalities					1951:1963	chromosome abnormalities	1940:1963	chromosome abnormalities	1940:1963	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	9	59	theme	µg/mL	1715:1719	arg1	MIC					1705:1707	MIC	1705:1707	MIC of 35 µg/mL	1705:1719	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	13	60	theme	procera	2288:2294	arg1	extract					2296:2302	C. procera extract	2285:2302	C. procera extract	2285:2302	Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.
35630600	7	61	dep	Klebsiella	1270:1279	arg1	pneumonia					1281:1289	Klebsiella pneumonia	1270:1289	Klebsiella pneumonia	1270:1289	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	12	62	theme	current	2022:2028	arg1	study					2030:2034	The current study	2018:2034	The current study	2018:2034	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	6	63	theme	antimicrobial	1011:1023	arg1	activity					1025:1032	C. procera plant extract's antimicrobial activity	984:1032	C. procera plant extract's antimicrobial activity	984:1032	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	0	64	theme	In	136:137	arg1	Antigenotoxicity					15:30	Antigenotoxicity	15:30	Antigenotoxicity	15:30	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	64	theme	In	136:137	arg1	Characterization					37:52	Characterization	37:52	Characterization	37:52	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	64	theme	In	136:137	arg1	Antimicrobial					0:12	Antimicrobial	0:12	Antimicrobial	0:12	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	64	theme	In	136:137	arg1	Studies					144:150	In Vitro and In Vivo Studies	123:150	In Vitro and In Vivo Studies	123:150	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	11	65	theme	extract	1854:1860	arg1	effect					1833:1838	The effect	1829:1838	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity	1829:1911	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	10	66	theme	gene	1736:1739	arg1	analysis					1750:1757	The 16S rRNA gene sequence analysis	1723:1757	The 16S rRNA gene sequence analysis	1723:1757	The 16S rRNA gene sequence analysis showed that the potential strains belonged to the genus Streptomyces.
35630600	11	67	theme	C.	1843:1844	arg1	extract					1854:1860	C. procera extract	1843:1860	C. procera extract	1843:1860	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	10	68	theme	potential	1775:1783	arg1	strains					1785:1791	the potential strains	1771:1791	the potential strains	1771:1791	The 16S rRNA gene sequence analysis showed that the potential strains belonged to the genus Streptomyces.
35630600	3	69	theme	carbon	587:592	arg1	source					594:599	a carbon source	585:599	a carbon source	585:599	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	9	70	theme	isolated	1536:1543	arg1	inhibitor					1593:1601	a moderate inhibitor	1582:1601	a moderate inhibitor against S. aureus (MIC of 86 µg/mL)	1582:1637	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	9	70	theme	isolated	1536:1543	arg1	actinobacteria					1545:1558	the isolated actinobacteria	1532:1558	the isolated actinobacteria	1532:1558	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	6	71	theme	minimum	1090:1096	arg1	MIC					1124:1126	MIC	1124:1126	MIC	1124:1126	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	6	71	theme	minimum	1090:1096	arg1	concentration					1109:1121	minimum inhibitory concentration	1090:1121	minimum inhibitory concentration (MIC)	1090:1127	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	9	72	theme	moderate	1584:1591	arg1	inhibitor					1593:1601	a moderate inhibitor	1582:1601	a moderate inhibitor against S. aureus (MIC of 86 µg/mL)	1582:1637	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	9	72	theme	moderate	1584:1591	arg1	actinobacteria					1545:1558	the isolated actinobacteria	1532:1558	the isolated actinobacteria	1532:1558	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	12	73	theme	mg/kg	2100:2104	arg1	procera					2074:2080	C. procera	2071:2080	C. procera (50, 100, and 200 mg/kg b.w.)	2071:2110	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	12	73	theme	mg/kg	2100:2104	arg1	b.w.					2106:2109	50, 100, and 200 mg/kg b.w.	2083:2109	50, 100, and 200 mg/kg b.w.	2083:2109	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	3	74	theme	agar	619:622	arg1	medium					624:629	starch-casein agar medium	605:629	starch-casein agar medium	605:629	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	0	75	dep	Studies	144:150	arg1	Antigenotoxicity					15:30	Antigenotoxicity	15:30	Antigenotoxicity	15:30	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	75	dep	Studies	144:150	arg1	Characterization					37:52	Characterization	37:52	Characterization	37:52	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	75	dep	Studies	144:150	arg1	Antimicrobial					0:12	Antimicrobial	0:12	Antimicrobial	0:12	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	75	dep	Studies	144:150	arg1	Studies					144:150	In Vitro and In Vivo Studies	123:150	In Vitro and In Vivo Studies	123:150	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	2	76	theme	rhizospheric	464:475	arg1	effects					492:498	its rhizospheric actinobacteria effects	460:498	its rhizospheric actinobacteria effects against pathogenic microorganisms	460:532	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	6	77	theme	agar	1060:1063	arg1	assay					1080:1084	an agar well-diffusion assay	1057:1084	an agar well-diffusion assay	1057:1084	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	0	78	theme	Rhizosphere-Inhabiting	84:105	arg1	Actinobacteria					107:120	Its Rhizosphere-Inhabiting Actinobacteria	80:120	Its Rhizosphere-Inhabiting Actinobacteria	80:120	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	0	79	dep	In	136:137	arg1	Vivo					139:142	Vivo	139:142	Vivo	139:142	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	9	80	theme	µg/mL	1632:1636	arg1	MIC					1622:1624	MIC	1622:1624	MIC of 86 µg/mL	1622:1636	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	2	81	theme	pathogenic	508:517	arg1	microorganisms					519:532	pathogenic microorganisms	508:532	pathogenic microorganisms	508:532	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	4	82	theme	chromatography/mass	756:774	arg1	spectrometry					776:787	gas chromatography/mass spectrometry	752:787	gas chromatography/mass spectrometry (GC-MS)	752:795	From the plant, a total of 31 compounds were identified using gas chromatography/mass spectrometry (GC-MS).
35630600	4	82	theme	chromatography/mass	756:774	arg1	GC-MS					790:794	GC-MS	790:794	GC-MS	790:794	From the plant, a total of 31 compounds were identified using gas chromatography/mass spectrometry (GC-MS).
35630600	12	83	theme	procera	2074:2080	arg1	pretreatment					2055:2066	oral pretreatment	2050:2066	oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals	2050:2168	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	2	84	theme	extract	378:384	arg1	composition					320:330	phytochemical composition	306:330	phytochemical composition	306:330	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	2	84	theme	extract	378:384	arg1	properties					350:359	antigenotoxic properties	336:359	antigenotoxic properties	336:359	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	11	85	theme	somatic	1974:1980	arg1	cells					1982:1986	mouse somatic cells	1968:1986	mouse somatic cells	1968:1986	The effect of C. procera extract against cyclophosphamide (CP)-induced genotoxicity was examined by evaluating chromosome abnormalities in mouse somatic cells and DNA fragmentation assays.
35630600	9	86	dep	albicans	1695:1702	arg1	MIC					1705:1707	MIC	1705:1707	MIC of 35 µg/mL	1705:1719	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	9	87	theme	potent	1646:1651	arg1	strain					1664:1669	strain CALT_2	1664:1676	strain CALT_2	1664:1676	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	9	87	theme	potent	1646:1651	arg1	inhibitor					1653:1661	a potent inhibitor	1644:1661	a potent inhibitor	1644:1661	On the other hand, the isolated actinobacteria were considered to be a moderate inhibitor against S. aureus (MIC of 86 µg/mL), and a potent inhibitor, strain CALT_2, against Candida albicans (MIC of 35 µg/mL).
35630600	8	88	theme	moderate	1428:1435	arg1	inhibitor					1437:1445	a moderate inhibitor	1426:1445	a moderate inhibitor against Bacillus subtilis	1426:1471	The plant extract was considered to be a moderate inhibitor against Bacillus subtilis, with MIC ranging from 0.60-1.50 mg/mL.
35630600	8	88	theme	moderate	1428:1435	arg1	extract					1397:1403	The plant extract	1387:1403	The plant extract	1387:1403	The plant extract was considered to be a moderate inhibitor against Bacillus subtilis, with MIC ranging from 0.60-1.50 mg/mL.
35630600	0	89	theme	Calotropis	57:66	arg1	procera					68:74	Calotropis procera	57:74	Calotropis procera	57:74	Antimicrobial, Antigenotoxicity, and Characterization of Calotropis procera and Its Rhizosphere-Inhabiting Actinobacteria: In Vitro and In Vivo Studies.
35630600	5	90	theme	linolenic	933:941	arg1	%					953:953	3.04%	949:953	3.04%	949:953	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	5	90	theme	linolenic	933:941	arg1	acid					943:946	linolenic acid	933:946	linolenic acid (3.04%)	933:954	The main components were α-amyrin (39.36%), lupeol acetate (17.94%), phytol (13.32%), hexadecanoic acid (5.55%), stigmasterol (3.16%), linolenic acid (3.04%), and gombasterol A (2.14%).
35630600	6	91	theme	plant	995:999	arg1	extract					1001:1007	C. procera plant extract	984:1007	C. procera plant extract's antimicrobial activity	984:1032	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	12	92	theme	chromosomal	2192:2202	arg1	abnormalities					2204:2216	chromosomal abnormalities	2192:2216	chromosomal abnormalities as well as DNA fragmentation	2192:2245	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	2	93	theme	phytochemical	306:318	arg1	composition					320:330	phytochemical composition	306:330	phytochemical composition	306:330	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
35630600	6	94	theme	C.	984:985	arg1	extract					1001:1007	C. procera plant extract	984:1007	C. procera plant extract's antimicrobial activity	984:1032	C. procera plant extract's antimicrobial activity was investigated using an agar well-diffusion assay and minimum inhibitory concentration (MIC) against six pathogenic microbial strains.
35630600	13	95	contain	had	2304:2306	arg2	effects					2340:2346	antimicrobial and antigenotoxic effects	2308:2346	antimicrobial and antigenotoxic effects	2308:2346	Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.
35630600	13	95	contain	had	2304:2306	arg1	extract					2296:2302	C. procera extract	2285:2302	C. procera extract	2285:2302	Moreover, C. procera extract had antimicrobial and antigenotoxic effects against CP-induced genotoxicity.
35630600	7	96	theme	mm	1369:1370	arg1	zones					1330:1334	inhibition zones	1319:1334	inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively	1319:1384	The plant extract of C. procera was considered significantly active against Staphylococcus aureus, Klebsiella pneumonia, and Escherichia coli, with inhibition zones of 18.66 mm, 21.26 mm, and 21.93 mm, respectively.
35630600	12	97	theme	cyclophosphamide-treated	2137:2160	arg1	animals					2162:2168	cyclophosphamide-treated animals	2137:2168	cyclophosphamide-treated animals	2137:2168	The current study revealed that oral pretreatment of C. procera (50, 100, and 200 mg/kg b.w.) for 1, 7, and 14 days to cyclophosphamide-treated animals significantly reduced chromosomal abnormalities as well as DNA fragmentation in a dose-dependent manner.
35630600	3	98	from	rhizosphere	677:687	arg1	isolation					644:652	isolation	644:652	isolation of actinobacteria from rhizosphere	644:687	Soil-extract medium supplemented with glycerol as a carbon source and starch-casein agar medium was used for isolation of actinobacteria from rhizosphere.
35630600	2	99	theme	antimicrobial	420:432	arg1	activity					434:441	the antimicrobial activity	416:441	the antimicrobial activity of the plant	416:454	In this study, we investigated the phytochemical composition and antigenotoxic properties of the ethanolic extract of C. procera, in addition to the antimicrobial activity of the plant and its rhizospheric actinobacteria effects against pathogenic microorganisms.
36252879	4	0	theme	study	521:525	arg1	purpose					505:511	The purpose	501:511	The purpose of this study	501:525	AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components.
36252879	9	1	theme	hub	1133:1135	arg1	targets					1137:1143	34 hub targets	1130:1143	34 hub targets between the 113 compounds and ALI	1130:1177	Network pharmacology revealed 34 hub targets between the 113 compounds and ALI.
36252879	6	2	theme	ALI	854:856	arg1	treatment					858:866	ALI treatment	854:866	ALI treatment	854:866	The potential targets of XFBD for ALI treatment were predicted using network pharmacological analysis.
36252879	2	3	theme	mild	254:257	arg1	syndrome					302:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	6	4	theme	XFBD	845:848	arg1	targets					834:840	The potential targets	820:840	The potential targets of XFBD for ALI treatment	820:866	The potential targets of XFBD for ALI treatment were predicted using network pharmacological analysis.
36252879	2	5	used	used	231:234	arg2	Formula					214:220	Xuanfei Baidu Formula	200:220	Xuanfei Baidu Formula(XFBD)	200:226	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	2	5	used	used	231:234	arg2	XFBD					222:225	XFBD	222:225	XFBD	222:225	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	0	6	theme	Xuanfei	0:6	arg1	Formula					14:20	Xuanfei Baidu Formula	0:20	Xuanfei Baidu Formula	0:20	Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
36252879	4	7	from	ALI	590:592	arg1	rats					597:600	rats	597:600	rats	597:600	AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components.
36252879	1	8	theme	common	166:171	arg1	manifestation					173:185	a common manifestation	164:185	a common manifestation of COVID-19	164:197	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	8	theme	common	166:171	arg1	injury					148:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury	106:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI)	106:159	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	2	9	theme	obstructive	280:290	arg1	syndrome					302:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	5	10	theme	quadrupole	775:784	arg1	spectrometry					806:817	quadrupole time-of-flight mass spectrometry	775:817	quadrupole time-of-flight mass spectrometry	775:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	7	11	theme	mouse	1025:1029	arg1	model					1035:1039	a mouse ALI model	1023:1039	a mouse ALI model	1023:1039	Finally, the molecular mechanism of XFBD was validated using a RAW264.7 cell inflammation model and a mouse ALI model.
36252879	5	12	theme	mass	801:804	arg1	spectrometry					806:817	quadrupole time-of-flight mass spectrometry	775:817	quadrupole time-of-flight mass spectrometry	775:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	6	13	theme	pharmacological	897:911	arg1	analysis					913:920	network pharmacological analysis	889:920	network pharmacological analysis	889:920	The potential targets of XFBD for ALI treatment were predicted using network pharmacological analysis.
36252879	1	14	theme	Acute	137:141	arg1	manifestation					173:185	a common manifestation	164:185	a common manifestation of COVID-19	164:197	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	14	theme	Acute	137:141	arg1	ALI					156:158	ALI	156:158	ALI	156:158	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	14	theme	Acute	137:141	arg1	injury					148:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury	106:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI)	106:159	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	7	15	theme	inflammation	1000:1011	arg1	model					1013:1017	a RAW264.7 cell inflammation model	984:1017	a RAW264.7 cell inflammation model	984:1017	Finally, the molecular mechanism of XFBD was validated using a RAW264.7 cell inflammation model and a mouse ALI model.
36252879	11	16	theme	LPS-induced	1420:1430	arg1	models					1441:1446	LPS-induced cellular models	1420:1446	LPS-induced cellular models	1420:1446	We found that XFBD reduced proinflammatory factor levels in LPS-induced cellular models.
36252879	5	17	theme	chemical	675:682	arg1	composition					684:694	the chemical composition	671:694	the chemical composition of XFBD	671:702	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	7	18	theme	molecular	936:944	arg1	mechanism					946:954	the molecular mechanism	932:954	the molecular mechanism of XFBD	932:962	Finally, the molecular mechanism of XFBD was validated using a RAW264.7 cell inflammation model and a mouse ALI model.
36252879	15	19	dep	CONCLUSION	1792:1801	arg1	identified					1814:1823	identified	1814:1823	identified	1814:1823	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	14	20	dep	In	1693:1694	arg1	vitro					1696:1700	vitro	1696:1700	vitro	1696:1700	In vitro experiments demonstrated that XFBD inhibited LPS-induced activation of the NF-κB pathway.
36252879	14	21	theme	NF-κB	1777:1781	arg1	pathway					1783:1789	the NF-κB pathway	1773:1789	the NF-κB pathway	1773:1789	In vitro experiments demonstrated that XFBD inhibited LPS-induced activation of the NF-κB pathway.
36252879	12	22	theme	lung	1466:1469	arg1	ratio					1486:1490	the lung wet/dry weight ratio	1462:1490	the lung wet/dry weight ratio	1462:1490	By examining the lung wet/dry weight ratio and pathological sections in vivo, XFBD was found that XFBD could alleviate ALI.
36252879	13	23	theme	p-NF-κB	1661:1667	arg1	p65					1669:1671	p-NF-κB p65	1661:1671	p-NF-κB p65	1661:1671	Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins.
36252879	0	24	theme	signaling	87:95	arg1	pathway					97:103	the NF-κB signaling pathway	77:103	the NF-κB signaling pathway	77:103	Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
36252879	12	25	theme	weight	1479:1484	arg1	ratio					1486:1490	the lung wet/dry weight ratio	1462:1490	the lung wet/dry weight ratio	1462:1490	By examining the lung wet/dry weight ratio and pathological sections in vivo, XFBD was found that XFBD could alleviate ALI.
36252879	0	26	theme	acute	45:49	arg1	injury					56:61	LPS-induced acute lung injury	33:61	LPS-induced acute lung injury	33:61	Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
36252879	15	27	theme	network	1883:1889	arg1	pharmacology					1891:1902	network pharmacology	1883:1902	network pharmacology	1883:1902	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	5	28	theme	performance	736:746	arg1	chromatography					755:768	ultra-high performance liquid chromatography	725:768	ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry	725:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	13	29	from	increases	1641:1649	arg1	proteins					1683:1690	iNOS proteins	1678:1690	iNOS proteins	1678:1690	Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins.
36252879	13	29	from	increases	1641:1649	arg1	p-IKK					1654:1658	p-IKK	1654:1658	p-IKK	1654:1658	Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins.
36252879	13	29	from	increases	1641:1649	arg1	p65					1669:1671	p-NF-κB p65	1661:1671	p-NF-κB p65	1661:1671	Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins.
36252879	15	30	theme	NF-κB	2015:2019	arg1	pathway					2031:2037	the NF-κB signaling pathway	2011:2037	the NF-κB signaling pathway	2011:2037	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	3	31	theme	active	346:351	arg1	ingredients					353:363	the active ingredients	342:363	the active ingredients of XFBD	342:371	However, the active ingredients of XFBD have not been extensively studied, and its mechanism of action in the treatment of ALI is not well understood.
36252879	4	32	theme	STUDY	495:499	arg1	AIM					484:486	AIM	484:486	AIM OF THE STUDY	484:499	AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components.
36252879	3	33	theme	action	429:434	arg1	mechanism					416:424	its mechanism	412:424	its mechanism of action in the treatment of ALI	412:458	However, the active ingredients of XFBD have not been extensively studied, and its mechanism of action in the treatment of ALI is not well understood.
36252879	6	34	theme	potential	824:832	arg1	targets					834:840	The potential targets	820:840	The potential targets of XFBD for ALI treatment	820:866	The potential targets of XFBD for ALI treatment were predicted using network pharmacological analysis.
36252879	1	35	theme	ETHNOPHARMACOLOGICAL	106:125	arg1	manifestation					173:185	a common manifestation	164:185	a common manifestation of COVID-19	164:197	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	35	theme	ETHNOPHARMACOLOGICAL	106:125	arg1	ALI					156:158	ALI	156:158	ALI	156:158	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	35	theme	ETHNOPHARMACOLOGICAL	106:125	arg1	injury					148:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury	106:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI)	106:159	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	11	36	theme	proinflammatory	1387:1401	arg1	levels					1410:1415	proinflammatory factor levels	1387:1415	proinflammatory factor levels	1387:1415	We found that XFBD reduced proinflammatory factor levels in LPS-induced cellular models.
36252879	0	37	theme	LPS-induced	33:43	arg1	injury					56:61	LPS-induced acute lung injury	33:61	LPS-induced acute lung injury	33:61	Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
36252879	10	38	theme	signaling	1287:1295	arg1	pathway					1297:1303	the NF-κB signaling pathway	1277:1303	the NF-κB signaling pathway	1277:1303	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	10	38	theme	signaling	1287:1295	arg1	pathway					1318:1324	the main pathway	1309:1324	the main pathway for XFBD	1309:1333	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	0	39	theme	lung	51:54	arg1	injury					56:61	LPS-induced acute lung injury	33:61	LPS-induced acute lung injury	33:61	Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
36252879	2	40	theme	Xuanfei	200:206	arg1	XFBD					222:225	XFBD	222:225	XFBD	222:225	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	2	40	theme	Xuanfei	200:206	arg1	Formula					214:220	Xuanfei Baidu Formula	200:220	Xuanfei Baidu Formula(XFBD)	200:226	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	8	41	theme	compounds	1065:1073	arg1	total					1052:1056	A total	1050:1056	RESULTS A total of 113 compounds	1042:1073	RESULTS A total of 113 compounds were identified in XFBD.
36252879	10	42	theme	main	1313:1316	arg1	pathway					1297:1303	the NF-κB signaling pathway	1277:1303	the NF-κB signaling pathway	1277:1303	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	10	42	theme	main	1313:1316	arg1	pathway					1318:1324	the main pathway	1309:1324	the main pathway for XFBD	1309:1333	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	14	43	theme	In	1693:1694	arg1	experiments					1702:1712	In vitro experiments	1693:1712	In vitro experiments	1693:1712	In vitro experiments demonstrated that XFBD inhibited LPS-induced activation of the NF-κB pathway.
36252879	9	44	theme	34	1130:1131	arg1	targets					1137:1143	34 hub targets	1130:1143	34 hub targets between the 113 compounds and ALI	1130:1177	Network pharmacology revealed 34 hub targets between the 113 compounds and ALI.
36252879	15	45	dep	potential	1829:1837	arg1	practical					1839:1847	practical	1839:1847	practical	1839:1847	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	10	46	theme	ALI	1355:1357	arg1	treatment					1342:1350	the treatment	1338:1350	the treatment of ALI	1338:1357	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	2	47	theme	pulmonary	292:300	arg1	syndrome					302:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	5	48	with	chromatography	755:768	arg1	spectrometry					806:817	quadrupole time-of-flight mass spectrometry	775:817	quadrupole time-of-flight mass spectrometry	775:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	15	49	theme	XFBD	1863:1866	arg1	components					1849:1858	the potential practical components	1825:1858	the potential practical components of XFBD	1825:1866	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	7	50	theme	ALI	1031:1033	arg1	model					1035:1039	a mouse ALI model	1023:1039	a mouse ALI model	1023:1039	Finally, the molecular mechanism of XFBD was validated using a RAW264.7 cell inflammation model and a mouse ALI model.
36252879	2	51	theme	damp-toxin	269:278	arg1	syndrome					302:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	mild or common damp-toxin obstructive pulmonary syndrome	254:309	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	1	52	theme	COVID-19	190:197	arg1	manifestation					173:185	a common manifestation	164:185	a common manifestation of COVID-19	164:197	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	52	theme	COVID-19	190:197	arg1	injury					148:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury	106:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI)	106:159	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	4	53	theme	action	563:568	arg1	mechanism					550:558	the mechanism	546:558	the mechanism of action of XFBD in treating ALI in rats	546:600	AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components.
36252879	4	54	dep	was	527:529	arg1	AIM					484:486	AIM	484:486	AIM OF THE STUDY	484:499	AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components.
36252879	5	55	theme	time-of-flight	786:799	arg1	spectrometry					806:817	quadrupole time-of-flight mass spectrometry	775:817	quadrupole time-of-flight mass spectrometry	775:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	4	56	theme	XFBD	573:576	arg1	action					563:568	action	563:568	action of XFBD in treating ALI in rats	563:600	AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components.
36252879	13	57	theme	iNOS	1678:1681	arg1	proteins					1683:1690	iNOS proteins	1678:1690	iNOS proteins	1678:1690	Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins.
36252879	10	58	theme	ontology	1244:1251	arg1	analyses					1253:1260	gene ontology analyses	1239:1260	gene ontology analyses	1239:1260	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	15	59	theme	potential	1829:1837	arg1	components					1849:1858	the potential practical components	1825:1858	the potential practical components of XFBD	1825:1866	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	1	60	theme	RELEVANCE	127:135	arg1	manifestation					173:185	a common manifestation	164:185	a common manifestation of COVID-19	164:197	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	60	theme	RELEVANCE	127:135	arg1	ALI					156:158	ALI	156:158	ALI	156:158	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	60	theme	RELEVANCE	127:135	arg1	injury					148:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury	106:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI)	106:159	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	6	61	theme	network	889:895	arg1	analysis					913:920	network pharmacological analysis	889:920	network pharmacological analysis	889:920	The potential targets of XFBD for ALI treatment were predicted using network pharmacological analysis.
36252879	4	62	theme	active	621:626	arg1	components					628:637	its active components	617:637	its active components	617:637	AIM OF THE STUDY The purpose of this study was to investigate the mechanism of action of XFBD in treating ALI in rats, by evaluating its active components.
36252879	11	63	theme	cellular	1432:1439	arg1	models					1441:1446	LPS-induced cellular models	1420:1446	LPS-induced cellular models	1420:1446	We found that XFBD reduced proinflammatory factor levels in LPS-induced cellular models.
36252879	7	64	theme	RAW264.7 cell	986:998	arg1	model					1013:1017	a RAW264.7 cell inflammation model	984:1017	a RAW264.7 cell inflammation model	984:1017	Finally, the molecular mechanism of XFBD was validated using a RAW264.7 cell inflammation model and a mouse ALI model.
36252879	1	65	theme	lung	143:146	arg1	manifestation					173:185	a common manifestation	164:185	a common manifestation of COVID-19	164:197	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	65	theme	lung	143:146	arg1	ALI					156:158	ALI	156:158	ALI	156:158	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	1	65	theme	lung	143:146	arg1	injury					148:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury	106:153	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI)	106:159	ETHNOPHARMACOLOGICAL RELEVANCE Acute lung injury (ALI) is a common manifestation of COVID-19.
36252879	12	66	theme	pathological	1496:1507	arg1	sections					1509:1516	pathological sections	1496:1516	pathological sections	1496:1516	By examining the lung wet/dry weight ratio and pathological sections in vivo, XFBD was found that XFBD could alleviate ALI.
36252879	7	67	theme	XFBD	959:962	arg1	mechanism					946:954	the molecular mechanism	932:954	the molecular mechanism of XFBD	932:962	Finally, the molecular mechanism of XFBD was validated using a RAW264.7 cell inflammation model and a mouse ALI model.
36252879	5	68	dep	MATERIALS	640:648	arg1	identified					708:717	identified	708:717	was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry	704:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	0	69	theme	NF-κB	81:85	arg1	pathway					97:103	the NF-κB signaling pathway	77:103	the NF-κB signaling pathway	77:103	Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
36252879	14	70	theme	pathway	1783:1789	arg1	activation					1759:1768	LPS-induced activation	1747:1768	LPS-induced activation of the NF-κB pathway	1747:1789	In vitro experiments demonstrated that XFBD inhibited LPS-induced activation of the NF-κB pathway.
36252879	12	71	theme	wet/dry	1471:1477	arg1	ratio					1486:1490	the lung wet/dry weight ratio	1462:1490	the lung wet/dry weight ratio	1462:1490	By examining the lung wet/dry weight ratio and pathological sections in vivo, XFBD was found that XFBD could alleviate ALI.
36252879	10	72	theme	Encyclopedia	1201:1212	arg1	results					1184:1190	The results	1180:1190	The results of Kyoto Encyclopedia of Genes and Genomes	1180:1233	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	10	72	theme	Encyclopedia	1201:1212	arg1	analyses					1253:1260	gene ontology analyses	1239:1260	gene ontology analyses	1239:1260	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	10	73	theme	gene	1239:1242	arg1	analyses					1253:1260	gene ontology analyses	1239:1260	gene ontology analyses	1239:1260	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	15	74	theme	signaling	2021:2029	arg1	pathway					2031:2037	the NF-κB signaling pathway	2011:2037	the NF-κB signaling pathway	2011:2037	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	13	75	theme	ALI-induced	1629:1639	arg1	increases					1641:1649	ALI-induced increases	1629:1649	ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins	1629:1690	Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins.
36252879	0	76	theme	Baidu	8:12	arg1	Formula					14:20	Xuanfei Baidu Formula	0:20	Xuanfei Baidu Formula	0:20	Xuanfei Baidu Formula attenuates LPS-induced acute lung injury by inhibiting the NF-κB signaling pathway.
36252879	3	77	theme	XFBD	368:371	arg1	ingredients					353:363	the active ingredients	342:363	the active ingredients of XFBD	342:371	However, the active ingredients of XFBD have not been extensively studied, and its mechanism of action in the treatment of ALI is not well understood.
36252879	11	78	theme	factor	1403:1408	arg1	levels					1410:1415	proinflammatory factor levels	1387:1415	proinflammatory factor levels	1387:1415	We found that XFBD reduced proinflammatory factor levels in LPS-induced cellular models.
36252879	5	79	theme	liquid	748:753	arg1	chromatography					755:768	ultra-high performance liquid chromatography	725:768	ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry	725:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	5	80	theme	XFBD	699:702	arg1	composition					684:694	the chemical composition	671:694	the chemical composition of XFBD	671:702	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36252879	14	81	theme	LPS-induced	1747:1757	arg1	activation					1759:1768	LPS-induced activation	1747:1768	LPS-induced activation of the NF-κB pathway	1747:1789	In vitro experiments demonstrated that XFBD inhibited LPS-induced activation of the NF-κB pathway.
36252879	10	82	from	pathway	1318:1324	arg1	treatment					1342:1350	the treatment	1338:1350	the treatment of ALI	1338:1357	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	3	83	from	mechanism	416:424	arg1	treatment					443:451	the treatment	439:451	the treatment of ALI	439:458	However, the active ingredients of XFBD have not been extensively studied, and its mechanism of action in the treatment of ALI is not well understood.
36252879	15	84	theme	lung	1971:1974	arg1	injury					1976:1981	lung injury	1971:1981	lung injury caused by ALI by inhibiting the NF-κB signaling pathway	1971:2037	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	2	85	theme	Baidu	208:212	arg1	XFBD					222:225	XFBD	222:225	XFBD	222:225	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	2	85	theme	Baidu	208:212	arg1	Formula					214:220	Xuanfei Baidu Formula	200:220	Xuanfei Baidu Formula(XFBD)	200:226	Xuanfei Baidu Formula(XFBD) is used in China to treat mild or common damp-toxin obstructive pulmonary syndrome in COVID-19 patients.
36252879	15	86	theme	experimental	1908:1919	arg1	validation					1921:1930	experimental validation	1908:1930	experimental validation	1908:1930	CONCLUSION This study identified the potential practical components of XFBD, combined with network pharmacology and experimental validation to demonstrate that XFBD can alleviate lung injury caused by ALI by inhibiting the NF-κB signaling pathway.
36252879	10	87	theme	NF-κB	1281:1285	arg1	pathway					1297:1303	the NF-κB signaling pathway	1277:1303	the NF-κB signaling pathway	1277:1303	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	10	87	theme	NF-κB	1281:1285	arg1	pathway					1318:1324	the main pathway	1309:1324	the main pathway for XFBD	1309:1333	The results of Kyoto Encyclopedia of Genes and Genomes and gene ontology analyses indicated that the NF-κB signaling pathway was the main pathway for XFBD in the treatment of ALI.
36252879	3	88	theme	ALI	456:458	arg1	treatment					443:451	the treatment	439:451	the treatment of ALI	439:458	However, the active ingredients of XFBD have not been extensively studied, and its mechanism of action in the treatment of ALI is not well understood.
36252879	13	89	theme	Immunohistochemistry	1573:1592	arg1	results					1594:1600	Immunohistochemistry results	1573:1600	Immunohistochemistry results	1573:1600	Immunohistochemistry results showed that XFBD inhibited ALI-induced increases in p-IKK, p-NF-κB p65, and iNOS proteins.
36252879	9	90	theme	Network	1100:1106	arg1	pharmacology					1108:1119	Network pharmacology	1100:1119	Network pharmacology	1100:1119	Network pharmacology revealed 34 hub targets between the 113 compounds and ALI.
36252879	8	91	dep	RESULTS	1042:1048	arg1	total					1052:1056	A total	1050:1056	RESULTS A total of 113 compounds	1042:1073	RESULTS A total of 113 compounds were identified in XFBD.
36252879	5	92	theme	ultra-high	725:734	arg1	chromatography					755:768	ultra-high performance liquid chromatography	725:768	ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry	725:817	MATERIALS AND METHODS Firstly, the chemical composition of XFBD was identified using ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry.
36077084	5	0	theme	AOM/DSS	740:746	arg1	mice					748:751	AOM/DSS mice	740:751	AOM/DSS mice	740:751	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	5	1	from	role	768:771	arg1	neoplasm					817:824	colitis-associated neoplasm	798:824	colitis-associated neoplasm	798:824	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	5	1	from	role	768:771	arg1	microbiota					831:840	gut microbiota	827:840	gut microbiota	827:840	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	5	1	from	role	768:771	arg1	cytokines					854:862	immune cytokines	847:862	immune cytokines	847:862	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	5	1	from	role	768:771	arg1	colitis					789:795	colitis	789:795	colitis	789:795	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	7	2	theme	rectal	1121:1126	arg1	tissue					1128:1133	rectal tissue	1121:1133	rectal tissue	1121:1133	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	7	3	theme	AOM/DSS	948:954	arg1	mice					956:959	AOM/DSS mice	948:959	AOM/DSS mice	948:959	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	4	4	theme	sulfate	571:577	arg1	protocol					527:534	the protocol	523:534	the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice	523:660	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	9	5	theme	normal	1283:1288	arg1	recipients					1290:1299	normal recipients	1283:1299	normal recipients	1283:1299	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	9	6	from	mice	1376:1379	arg1	FMT					1392:1394	inverse FMT	1384:1394	inverse FMT	1384:1394	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	7	7	theme	CBM	928:930	arg1	intervention					932:943	The CBM intervention	924:943	The CBM intervention on AOM/DSS mice	924:959	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	7	8	from	intervention	932:943	arg1	mice					956:959	AOM/DSS mice	948:959	AOM/DSS mice	948:959	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	4	9	theme	sodium	564:569	arg1	AOM/DSS					580:586	AOM/DSS	580:586	AOM/DSS	580:586	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	4	9	theme	sodium	564:569	arg1	sulfate					571:577	dextran sodium sulfate	556:577	dextran sodium sulfate (AOM/DSS)	556:587	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	5	10	theme	butyricum	713:721	arg1	CBM					732:734	CBM	732:734	CBM	732:734	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	5	10	theme	butyricum	713:721	arg1	Miyairi					723:729	Clostridium butyricum Miyairi	701:729	Clostridium butyricum Miyairi (CBM) on AOM/DSS mice	701:751	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	7	11	theme	IL-17A	1111:1116	arg1	composition					1042:1052	microbial composition	1032:1052	microbial composition	1032:1052	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	7	11	theme	IL-17A	1111:1116	arg1	expression					1081:1090	increased expression	1071:1090	increased expression of proinflammatory IL-17A in rectal tissue	1071:1133	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	11	12	theme	probiotics	1536:1545	arg1	role					1528:1531	The role	1524:1531	The role of probiotics in IBD	1524:1552	The role of probiotics in IBD requires further elucidation.
36077084	9	13	theme	colon	1420:1424	arg1	neoplasms					1426:1434	colon neoplasms	1420:1434	colon neoplasms	1420:1434	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	0	14	theme	Dextran	108:114	arg1	Sulfate					123:129	Dextran Sodium Sulfate	108:129	Dextran Sodium Sulfate	108:129	Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model.
36077084	0	15	from	Protection	39:48	arg1	Disease					72:78	Inflammatory Bowel Disease	53:78	Inflammatory Bowel Disease	53:78	Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model.
36077084	5	16	theme	colitis-associated	798:815	arg1	neoplasm					817:824	colitis-associated neoplasm	798:824	colitis-associated neoplasm	798:824	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	3	17	theme	murine	458:463	arg1	model					465:469	a simulated IBD murine model	442:469	a simulated IBD murine model	442:469	We performed a probiotic intervention on a simulated IBD murine model to clarify their relationship.
36077084	0	18	dep	Azoxymethane	91:102	arg1	Model					138:142	Murine Model	131:142	Murine Model	131:142	Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model.
36077084	2	19	theme	IBD	387:389	arg1	patients					391:398	IBD patients	387:398	IBD patients	387:398	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	10	20	theme	probiotic	1453:1461	arg1	intervention					1463:1474	the probiotic intervention	1449:1474	the probiotic intervention	1449:1474	We presumed the probiotic intervention to some extent caused dysbiosis as inverse FMT.
36077084	9	21	theme	pathogenic	1317:1326	arg1	effect					1328:1333	the pathogenic effect	1313:1333	the pathogenic effect of dysbiosis from AOM/DSS mice	1313:1364	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	7	22	theme	proinflammatory	1095:1109	arg1	IL-17A					1111:1116	proinflammatory IL-17A	1095:1116	proinflammatory IL-17A	1095:1116	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	9	23	theme	inverse	1238:1244	arg1	FMT					1246:1248	inverse FMT	1238:1248	inverse FMT	1238:1248	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	4	24	from	model	641:645	arg1	mice					657:660	BALB/c mice	650:660	BALB/c mice	650:660	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	4	25	theme	neoplasm	632:639	arg1	model					641:645	a colitis and colitis-associated neoplasm model	599:645	model	641:645	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	7	26	theme	increased	1071:1079	arg1	expression					1081:1090	increased expression	1071:1090	increased expression of proinflammatory IL-17A in rectal tissue	1071:1133	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	11	27	theme	further	1563:1569	arg1	elucidation					1571:1581	further elucidation	1563:1581	further elucidation	1563:1581	The role of probiotics in IBD requires further elucidation.
36077084	4	28	theme	BALB/c	650:655	arg1	mice					657:660	BALB/c mice	650:660	BALB/c mice	650:660	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	5	29	theme	probiotic	776:784	arg1	role					768:771	the role	764:771	the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines	764:862	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	4	30	theme	azoxymethane	539:550	arg1	protocol					527:534	the protocol	523:534	the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice	523:660	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	2	31	dep	trying	243:248	arg1	restore					253:259	restore	253:259	to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention	250:348	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	7	32	from	expression	1081:1090	arg1	tissue					1128:1133	rectal tissue	1121:1133	rectal tissue	1121:1133	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	5	33	theme	probiotic	672:680	arg1	intervention					682:693	A single probiotic intervention	663:693	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines	663:862	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	2	34	theme	microbiota	291:300	arg1	FMT					319:321	FMT	319:321	FMT	319:321	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	2	34	theme	microbiota	291:300	arg1	transplantation					302:316	fecal microbiota transplantation	285:316	fecal microbiota transplantation (FMT)	285:322	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	3	35	theme	IBD	454:456	arg1	model					465:469	a simulated IBD murine model	442:469	a simulated IBD murine model	442:469	We performed a probiotic intervention on a simulated IBD murine model to clarify their relationship.
36077084	4	36	theme	dextran	556:562	arg1	AOM/DSS					580:586	AOM/DSS	580:586	AOM/DSS	580:586	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	4	36	theme	dextran	556:562	arg1	sulfate					571:577	dextran sodium sulfate	556:577	dextran sodium sulfate (AOM/DSS)	556:587	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	0	37	theme	Sodium	116:121	arg1	Sulfate					123:129	Dextran Sodium Sulfate	108:129	Dextran Sodium Sulfate	108:129	Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model.
36077084	2	38	theme	fecal	285:289	arg1	FMT					319:321	FMT	319:321	FMT	319:321	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	2	38	theme	fecal	285:289	arg1	transplantation					302:316	fecal microbiota transplantation	285:316	fecal microbiota transplantation (FMT)	285:322	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	4	39	from	colitis	601:607	arg1	mice					657:660	BALB/c mice	650:660	BALB/c mice	650:660	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	1	40	theme	inflammatory	200:211	arg1	IBD					228:230	IBD	228:230	IBD	228:230	Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD).
36077084	1	40	theme	inflammatory	200:211	arg1	disease					219:225	inflammatory bowel disease	200:225	inflammatory bowel disease (IBD)	200:231	Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD).
36077084	9	41	theme	AOM/DSS	1353:1359	arg1	mice					1361:1364	AOM/DSS mice	1353:1364	AOM/DSS mice	1353:1364	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	7	42	theme	microbial	1032:1040	arg1	composition					1042:1052	microbial composition	1032:1052	microbial composition	1032:1052	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	4	43	theme	colitis-associated	613:630	arg1	model					641:645	a colitis and colitis-associated neoplasm model	599:645	model	641:645	IBD was simulated by the protocol of azoxymethane and dextran sodium sulfate (AOM/DSS) to set up a colitis and colitis-associated neoplasm model on BALB/c mice.
36077084	3	44	theme	probiotic	416:424	arg1	intervention					426:437	a probiotic intervention	414:437	a probiotic intervention	414:437	We performed a probiotic intervention on a simulated IBD murine model to clarify their relationship.
36077084	1	45	theme	bowel	213:217	arg1	IBD					228:230	IBD	228:230	IBD	228:230	Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD).
36077084	1	45	theme	bowel	213:217	arg1	disease					219:225	inflammatory bowel disease	200:225	inflammatory bowel disease (IBD)	200:231	Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD).
36077084	5	46	theme	gut	827:829	arg1	microbiota					831:840	gut microbiota	827:840	gut microbiota	827:840	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	0	47	theme	Murine	131:136	arg1	Model					138:142	Murine Model	131:142	Murine Model	131:142	Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model.
36077084	9	48	theme	dysbiosis	1338:1346	arg1	effect					1328:1333	the pathogenic effect	1313:1333	the pathogenic effect of dysbiosis from AOM/DSS mice	1313:1364	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	2	49	from	benefit	376:382	arg1	patients					391:398	IBD patients	387:398	IBD patients	387:398	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	3	50	theme	simulated	444:452	arg1	model					465:469	a simulated IBD murine model	442:469	a simulated IBD murine model	442:469	We performed a probiotic intervention on a simulated IBD murine model to clarify their relationship.
36077084	2	51	theme	clinical	367:374	arg1	benefit					376:382	clinical benefit	367:382	clinical benefit in IBD patients	367:398	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	5	52	from	Miyairi	723:729	arg1	mice					748:751	AOM/DSS mice	740:751	AOM/DSS mice	740:751	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	10	53	theme	inverse	1511:1517	arg1	FMT					1519:1521	inverse FMT	1511:1521	inverse FMT	1511:1521	We presumed the probiotic intervention to some extent caused dysbiosis as inverse FMT.
36077084	2	54	theme	probiotic	327:335	arg1	intervention					337:348	probiotic intervention	327:348	probiotic intervention	327:348	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	5	55	theme	Clostridium	701:711	arg1	butyricum					713:721	Clostridium butyricum	701:721	Clostridium butyricum Miyairi (CBM) on AOM/DSS mice	701:751	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	2	56	theme	microbial	261:269	arg1	diversity					271:279	microbial diversity	261:279	microbial diversity	261:279	However, trying to restore microbial diversity via fecal microbiota transplantation (FMT) or probiotic intervention fails to achieve clinical benefit in IBD patients.
36077084	9	57	from	mice	1361:1364	arg1	effect					1328:1333	the pathogenic effect	1313:1333	the pathogenic effect of dysbiosis from AOM/DSS mice	1313:1364	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	7	58	theme	colitis-associated	991:1008	arg1	neoplasms					1010:1018	colitis-associated neoplasms	991:1018	colitis-associated neoplasms	991:1018	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	8	59	theme	probiotic	1161:1169	arg1	intervention					1171:1182	the probiotic intervention	1157:1182	the probiotic intervention	1157:1182	We hypothesized that the probiotic intervention caused dysbiosis.
36077084	5	60	theme	single	665:670	arg1	intervention					682:693	A single probiotic intervention	663:693	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines	663:862	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	9	61	theme	AOM/DSS	1267:1273	arg1	mice					1275:1278	AOM/DSS mice	1267:1278	AOM/DSS mice	1267:1278	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	11	62	from	role	1528:1531	arg1	IBD					1550:1552	IBD	1550:1552	IBD	1550:1552	The role of probiotics in IBD requires further elucidation.
36077084	9	63	theme	inverse	1384:1390	arg1	FMT					1392:1394	inverse FMT	1384:1394	inverse FMT	1384:1394	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	0	64	theme	Bowel	66:70	arg1	Disease					72:78	Inflammatory Bowel Disease	53:78	Inflammatory Bowel Disease	53:78	Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model.
36077084	7	65	from	composition	1042:1052	arg1	tissue					1128:1133	rectal tissue	1121:1133	rectal tissue	1121:1133	The CBM intervention on AOM/DSS mice failed to improve colitis and colitis-associated neoplasms but changed microbial composition and unexpectedly increased expression of proinflammatory IL-17A in rectal tissue.
36077084	5	66	theme	immune	847:852	arg1	cytokines					854:862	immune cytokines	847:862	immune cytokines	847:862	A single probiotic intervention using Clostridium butyricum Miyairi (CBM) on AOM/DSS mice to clarify the role of probiotic in colitis, colitis-associated neoplasm, gut microbiota, and immune cytokines was performed.
36077084	0	67	theme	Inflammatory	53:64	arg1	Disease					72:78	Inflammatory Bowel Disease	53:78	Inflammatory Bowel Disease	53:78	Presume Why Probiotics May Not Provide Protection in Inflammatory Bowel Disease through an Azoxymethane and Dextran Sodium Sulfate Murine Model.
36077084	9	68	from	mice	1275:1278	arg1	feces					1256:1260	feces	1256:1260	feces from AOM/DSS mice to normal recipients	1256:1299	To clarify the result, we performed inverse FMT using feces from AOM/DSS mice to normal recipients to validate the pathogenic effect of dysbiosis from AOM/DSS mice and found mice on inverse FMT did develop colitis and colon neoplasms.
36077084	1	69	theme	Recent	145:150	arg1	studies					152:158	Recent studies	145:158	Recent studies	145:158	Recent studies have shown dysbiosis is associated with inflammatory bowel disease (IBD).
36077084	6	70	theme	AOM/DSS	910:916	arg1	mice					918:921	AOM/DSS mice	910:921	AOM/DSS mice	910:921	We found dysbiosis occurred in AOM/DSS mice.
35269806	7	0	theme	tight	1371:1375	arg1	ZO-1					1410:1413	ZO-1	1410:1413	ZO-1	1410:1413	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	0	theme	tight	1371:1375	arg1	claudin-1					1396:1404	claudin-1	1396:1404	claudin-1	1396:1404	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	0	theme	tight	1371:1375	arg1	proteins					1386:1393	tight junction proteins	1371:1393	tight junction proteins (claudin-1 and ZO-1)	1371:1414	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	1	dep	proteins	1386:1393	arg1	ZO-1					1410:1413	ZO-1	1410:1413	ZO-1	1410:1413	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	1	dep	proteins	1386:1393	arg1	claudin-1					1396:1404	claudin-1	1396:1404	claudin-1	1396:1404	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	1	dep	proteins	1386:1393	arg1	proteins					1386:1393	tight junction proteins	1371:1393	tight junction proteins (claudin-1 and ZO-1)	1371:1414	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	2	theme	Cyclin	1327:1332	arg1	D1					1334:1335	Cyclin D1	1327:1335	Cyclin D1	1327:1335	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	10	3	theme	carcinogenesis	2047:2060	arg1	regulation					2019:2028	regulation	2019:2028	regulation of inflammation, carcinogenesis, and compositional change of gut microbiota	2019:2104	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	9	4	dep	Saccharimonas	1714:1726	arg1	Patescibacteria					1686:1700	Patescibacteria	1686:1700	Patescibacteria	1686:1700	The comparative analysis of gut microbiota revealed that Bacteroidetes, Patescibacteria, Candidatus Saccharimonas, Erysipelatoclostridium, and Enterorhabdus were significantly increased but Firmicutes, Turicibacter, Romboutsia, and Blautia decreased after corylin treatment.
35269806	1	5	theme	microbiota	283:292	arg1	dysbiosis					294:302	gastrointestinal microbiota dysbiosis	266:302	gastrointestinal microbiota dysbiosis	266:302	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	4	6	theme	/DSS-induced	797:808	arg1	model					820:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	10	7	theme	gut	2091:2093	arg1	microbiota					2095:2104	gut microbiota	2091:2104	gut microbiota	2091:2104	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	4	8	theme	mouse	814:818	arg1	model					820:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	7	9	theme	layer	1314:1318	arg1	proliferation					1287:1299	epithelial cell proliferation	1271:1299	epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4)	1271:1347	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	10	10	theme	change	2081:2086	arg1	regulation					2019:2028	regulation	2019:2028	regulation of inflammation, carcinogenesis, and compositional change of gut microbiota	2019:2104	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	6	11	theme	signaling-associated	1149:1168	arg1	MyD88					1185:1189	MyD88	1185:1189	MyD88	1185:1189	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	11	theme	signaling-associated	1149:1168	arg1	F4/80					1209:1213	F4/80	1209:1213	F4/80	1209:1213	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	11	theme	signaling-associated	1149:1168	arg1	AP-1					1192:1195	AP-1	1192:1195	AP-1	1192:1195	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	11	theme	signaling-associated	1149:1168	arg1	markers					1170:1176	inflammatory signaling-associated markers	1136:1176	inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80)	1136:1214	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	11	theme	signaling-associated	1149:1168	arg1	TLR4					1179:1182	TLR4	1179:1182	TLR4	1179:1182	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	11	theme	signaling-associated	1149:1168	arg1	CD11b					1198:1202	CD11b	1198:1202	CD11b	1198:1202	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	9	12	theme	corylin	1870:1876	arg1	treatment					1878:1886	corylin treatment	1870:1886	corylin treatment	1870:1886	The comparative analysis of gut microbiota revealed that Bacteroidetes, Patescibacteria, Candidatus Saccharimonas, Erysipelatoclostridium, and Enterorhabdus were significantly increased but Firmicutes, Turicibacter, Romboutsia, and Blautia decreased after corylin treatment.
35269806	5	13	theme	AOM/DSS	998:1004	arg1	group					1006:1010	the AOM/DSS group	994:1010	the AOM/DSS group	994:1010	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	1	14	theme	chronic	210:216	arg1	inflammation					218:229	chronic inflammation	210:229	chronic inflammation	210:229	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	2	15	theme	colitis	513:519	arg1	model					467:471	a mouse model	459:471	a mouse model of dextran sodium sulfate (DSS)-induced colitis	459:519	In this study, we evaluated the effects of corylin in a mouse model of dextran sodium sulfate (DSS)-induced colitis.
35269806	7	16	theme	epithelial	1271:1280	arg1	proliferation					1287:1299	epithelial cell proliferation	1271:1299	epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4)	1271:1347	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	10	17	theme	cancer-ameliorating	1931:1949	arg1	effects					1951:1957	cancer-ameliorating effects	1931:1957	cancer-ameliorating effects	1931:1957	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	5	18	theme	tissue	878:883	arg1	barrier					885:891	the colon tissue barrier	868:891	the colon tissue barrier	868:891	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	6	19	theme	inflammatory	1136:1147	arg1	MyD88					1185:1189	MyD88	1185:1189	MyD88	1185:1189	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	19	theme	inflammatory	1136:1147	arg1	F4/80					1209:1213	F4/80	1209:1213	F4/80	1209:1213	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	19	theme	inflammatory	1136:1147	arg1	AP-1					1192:1195	AP-1	1192:1195	AP-1	1192:1195	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	19	theme	inflammatory	1136:1147	arg1	markers					1170:1176	inflammatory signaling-associated markers	1136:1176	inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80)	1136:1214	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	19	theme	inflammatory	1136:1147	arg1	TLR4					1179:1182	TLR4	1179:1182	TLR4	1179:1182	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	19	theme	inflammatory	1136:1147	arg1	CD11b					1198:1202	CD11b	1198:1202	CD11b	1198:1202	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	9	20	theme	gut	1642:1644	arg1	microbiota					1646:1655	gut microbiota	1642:1655	gut microbiota	1642:1655	The comparative analysis of gut microbiota revealed that Bacteroidetes, Patescibacteria, Candidatus Saccharimonas, Erysipelatoclostridium, and Enterorhabdus were significantly increased but Firmicutes, Turicibacter, Romboutsia, and Blautia decreased after corylin treatment.
35269806	7	21	theme	barrier	1238:1244	arg1	experiment					1246:1255	a colon barrier experiment	1230:1255	a colon barrier experiment	1230:1255	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	6	22	dep	markers	1170:1176	arg1	MyD88					1185:1189	MyD88	1185:1189	MyD88	1185:1189	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	22	dep	markers	1170:1176	arg1	F4/80					1209:1213	F4/80	1209:1213	F4/80	1209:1213	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	22	dep	markers	1170:1176	arg1	AP-1					1192:1195	AP-1	1192:1195	AP-1	1192:1195	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	22	dep	markers	1170:1176	arg1	markers					1170:1176	inflammatory signaling-associated markers	1136:1176	inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80)	1136:1214	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	22	dep	markers	1170:1176	arg1	TLR4					1179:1182	TLR4	1179:1182	TLR4	1179:1182	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	22	dep	markers	1170:1176	arg1	CD11b					1198:1202	CD11b	1198:1202	CD11b	1198:1202	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	0	23	theme	Intestinal	59:68	arg1	Junction					76:83	Intestinal Tight Junction	59:83	Intestinal Tight Junction	59:83	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	8	24	dep	diversity	1535:1543	arg1	the					1521:1523	the	1521:1523	the	1521:1523	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	3	25	theme	colon	586:590	arg1	length					592:597	colon length	586:597	colon length	586:597	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	3	26	dep	showed	534:539	arg1	ameliorated					628:638	ameliorated	628:638	ameliorated the inflammatory response in the colon	628:677	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	3	26	dep	showed	534:539	arg1	improved					555:562	improved	555:562	improved the survival rate and colon length	555:597	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	3	26	dep	showed	534:539	arg1	maintained					600:609	maintained	600:609	maintained body weight	600:621	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	0	27	theme	Junction	76:83	arg1	Upregulation					43:54	Upregulation	43:54	Upregulation of Intestinal Tight Junction	43:83	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	1	28	theme	bowel	181:185	arg1	IBD					196:198	IBD	196:198	IBD	196:198	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	1	28	theme	bowel	181:185	arg1	disease					187:193	Inflammatory bowel disease	168:193	Inflammatory bowel disease (IBD)	168:199	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	2	29	from	effects	437:443	arg1	model					467:471	a mouse model	459:471	a mouse model of dextran sodium sulfate (DSS)-induced colitis	459:519	In this study, we evaluated the effects of corylin in a mouse model of dextran sodium sulfate (DSS)-induced colitis.
35269806	10	30	theme	colon	2002:2006	arg1	cancer					2008:2013	colitis-associated colon cancer	1983:2013	colitis-associated colon cancer	1983:2013	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	0	31	theme	Anti-Inflammatory	94:110	arg1	Effect					112:117	Anti-Inflammatory Effect	94:117	Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice	94:165	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	1	32	theme	colitis-associated	361:378	arg1	CAC					399:401	CAC	399:401	CAC	399:401	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	1	32	theme	colitis-associated	361:378	arg1	cancer					391:396	colitis-associated colorectal cancer	361:396	colitis-associated colorectal cancer (CAC)	361:402	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	4	33	from	effects	731:737	arg1	model					820:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	4	34	theme	30-day	745:750	arg1	treatment					752:760	30-day treatment	745:760	30-day treatment of corylin	745:771	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	7	35	theme	colon	1232:1236	arg1	experiment					1246:1255	a colon barrier experiment	1230:1255	a colon barrier experiment	1230:1255	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	4	36	theme	possible	712:719	arg1	effects					731:737	the possible antitumor effects	708:737	the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model	708:824	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	0	37	theme	Gut	14:16	arg1	Microbiota					18:27	Gut Microbiota	14:27	Gut Microbiota	14:27	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	2	38	theme	mouse	461:465	arg1	model					467:471	a mouse model	459:471	a mouse model of dextran sodium sulfate (DSS)-induced colitis	459:519	In this study, we evaluated the effects of corylin in a mouse model of dextran sodium sulfate (DSS)-induced colitis.
35269806	4	39	theme	azoxymethane	779:790	arg1	model					820:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	3	40	theme	body	611:614	arg1	weight					616:621	body weight	611:621	body weight	611:621	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	1	41	theme	colon	339:343	arg1	cancer					345:350	a colon cancer	337:350	a colon cancer known as colitis-associated colorectal cancer (CAC)	337:402	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	3	42	theme	inflammatory	644:655	arg1	response					657:664	the inflammatory response	640:664	the inflammatory response in the colon	640:677	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	6	43	dep	cytokines	1074:1082	arg1	cytokines					1074:1082	pro-inflammatory cytokines	1057:1082	pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression	1057:1130	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	43	dep	cytokines	1074:1082	arg1	IL-1β					1099:1103	IL-1β	1099:1103	IL-1β	1099:1103	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	43	dep	cytokines	1074:1082	arg1	IL-6					1110:1113	IL-6	1110:1113	IL-6	1110:1113	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	43	dep	cytokines	1074:1082	arg1	IFN-γ					1092:1096	IFN-γ	1092:1096	IFN-γ	1092:1096	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	43	dep	cytokines	1074:1082	arg1	TNF-α					1085:1089	TNF-α	1085:1089	TNF-α	1085:1089	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	7	44	theme	junction	1377:1384	arg1	ZO-1					1410:1413	ZO-1	1410:1413	ZO-1	1410:1413	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	44	theme	junction	1377:1384	arg1	claudin-1					1396:1404	claudin-1	1396:1404	claudin-1	1396:1404	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	44	theme	junction	1377:1384	arg1	proteins					1386:1393	tight junction proteins	1371:1393	tight junction proteins (claudin-1 and ZO-1)	1371:1414	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	6	45	theme	pro-inflammatory	1057:1072	arg1	cytokines					1074:1082	pro-inflammatory cytokines	1057:1082	pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression	1057:1130	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	45	theme	pro-inflammatory	1057:1072	arg1	IL-1β					1099:1103	IL-1β	1099:1103	IL-1β	1099:1103	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	45	theme	pro-inflammatory	1057:1072	arg1	IL-6					1110:1113	IL-6	1110:1113	IL-6	1110:1113	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	45	theme	pro-inflammatory	1057:1072	arg1	IFN-γ					1092:1096	IFN-γ	1092:1096	IFN-γ	1092:1096	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	6	45	theme	pro-inflammatory	1057:1072	arg1	TNF-α					1085:1089	TNF-α	1085:1089	TNF-α	1085:1089	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	0	46	from	Cancer	152:157	arg1	Mice					162:165	Mice	162:165	Mice	162:165	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	1	47	theme	epithelial	240:249	arg1	integrity					251:259	epithelial integrity	240:259	epithelial integrity	240:259	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	10	48	theme	compositional	2067:2079	arg1	change					2081:2086	compositional change	2067:2086	compositional change of gut microbiota	2067:2104	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	8	49	theme	mice	1583:1586	arg1	richness					1559:1566	community richness	1549:1566	community richness	1549:1566	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	8	49	theme	mice	1583:1586	arg1	diversity					1535:1543	microbial diversity	1525:1543	microbial diversity	1525:1543	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	1	50	theme	gastrointestinal	266:281	arg1	dysbiosis					294:302	gastrointestinal microbiota dysbiosis	266:302	gastrointestinal microbiota dysbiosis	266:302	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	7	51	dep	layer	1314:1318	arg1	Olfm4					1342:1346	Olfm4	1342:1346	Olfm4	1342:1346	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	51	dep	layer	1314:1318	arg1	Lgr5					1321:1324	Lgr5	1321:1324	Lgr5	1321:1324	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	7	51	dep	layer	1314:1318	arg1	D1					1334:1335	Cyclin D1	1327:1335	Cyclin D1	1327:1335	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	10	52	theme	microbiota	2095:2104	arg1	inflammation					2033:2044	inflammation	2033:2044	inflammation	2033:2044	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	10	52	theme	microbiota	2095:2104	arg1	change					2081:2086	compositional change	2067:2086	compositional change of gut microbiota	2067:2104	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	10	52	theme	microbiota	2095:2104	arg1	carcinogenesis					2047:2060	carcinogenesis	2047:2060	carcinogenesis	2047:2060	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	8	53	theme	microbial	1525:1533	arg1	diversity					1535:1543	microbial diversity	1525:1543	microbial diversity	1525:1543	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	4	54	theme	CAC	810:812	arg1	model					820:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	an azoxymethane (AOM)/DSS-induced CAC mouse model	776:824	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	10	55	theme	corylin	1901:1907	arg1	administration					1909:1922	corylin administration	1901:1922	corylin administration	1901:1922	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	0	56	theme	Colitis-Associated	133:150	arg1	Cancer					152:157	Colitis-Associated Cancer	133:157	Colitis-Associated Cancer in Mice	133:165	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	7	57	theme	mucus	1308:1312	arg1	layer					1314:1318	the mucus layer	1304:1318	the mucus layer (Lgr5, Cyclin D1, and Olfm4)	1304:1347	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	3	58	from	response	657:664	arg1	colon					673:677	the colon	669:677	the colon	669:677	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	3	59	theme	survival	568:575	arg1	rate					577:580	the survival rate	564:580	the survival rate	564:580	The results showed corylin could improved the survival rate and colon length, maintained body weight, and ameliorated the inflammatory response in the colon.
35269806	2	60	theme	-induced	504:511	arg1	colitis					513:519	dextran sodium sulfate (DSS)-induced colitis	476:519	dextran sodium sulfate (DSS)-induced colitis	476:519	In this study, we evaluated the effects of corylin in a mouse model of dextran sodium sulfate (DSS)-induced colitis.
35269806	5	61	theme	corylin	1019:1025	arg1	groups					1027:1032	corylin groups	1019:1032	corylin groups	1019:1032	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	5	62	theme	colon	872:876	arg1	barrier					885:891	the colon tissue barrier	868:891	the colon tissue barrier	868:891	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	9	63	theme	comparative	1618:1628	arg1	analysis					1630:1637	The comparative analysis	1614:1637	The comparative analysis of gut microbiota	1614:1655	The comparative analysis of gut microbiota revealed that Bacteroidetes, Patescibacteria, Candidatus Saccharimonas, Erysipelatoclostridium, and Enterorhabdus were significantly increased but Firmicutes, Turicibacter, Romboutsia, and Blautia decreased after corylin treatment.
35269806	7	64	theme	cell	1282:1285	arg1	proliferation					1287:1299	epithelial cell proliferation	1271:1299	epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4)	1271:1347	In addition, a colon barrier experiment revealed that epithelial cell proliferation of the mucus layer (Lgr5, Cyclin D1, and Olfm4) was downregulated and tight junction proteins (claudin-1 and ZO-1) were upregulated.
35269806	0	65	from	Effect	112:117	arg1	Cancer					152:157	Colitis-Associated Cancer	133:157	Colitis-Associated Cancer in Mice	133:165	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	0	66	theme	Tight	70:74	arg1	Junction					76:83	Intestinal Tight Junction	59:83	Intestinal Tight Junction	59:83	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	9	67	theme	microbiota	1646:1655	arg1	analysis					1630:1637	The comparative analysis	1614:1637	The comparative analysis of gut microbiota	1614:1655	The comparative analysis of gut microbiota revealed that Bacteroidetes, Patescibacteria, Candidatus Saccharimonas, Erysipelatoclostridium, and Enterorhabdus were significantly increased but Firmicutes, Turicibacter, Romboutsia, and Blautia decreased after corylin treatment.
35269806	6	68	theme	mRNA	1116:1119	arg1	expression					1121:1130	pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression	1057:1130	pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression	1057:1130	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	1	69	theme	cancer	345:350	arg1	development					322:332	the development	318:332	the development of a colon cancer known as colitis-associated colorectal cancer (CAC)	318:402	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	1	70	theme	Inflammatory	168:179	arg1	IBD					196:198	IBD	196:198	IBD	196:198	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	1	70	theme	Inflammatory	168:179	arg1	disease					187:193	Inflammatory bowel disease	168:193	Inflammatory bowel disease (IBD)	168:199	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	5	71	dep	polarization	905:916	arg1	CD206					943:947	CD206	943:947	CD206	943:947	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	5	71	dep	polarization	905:916	arg1	CCR7					926:929	CCR7	926:929	CCR7	926:929	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	5	71	dep	polarization	905:916	arg1	CD11c					919:923	CD11c	919:923	CD11c	919:923	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	5	71	dep	polarization	905:916	arg1	CD163					932:936	CD163	932:936	CD163	932:936	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	10	72	theme	cancer	2008:2013	arg1	risk					1975:1978	the risk	1971:1978	the risk of colitis-associated colon cancer	1971:2013	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	11	73	dep	novel	2137:2141	arg1	natural					2173:2179	natural	2173:2179	natural	2173:2179	Therefore, corylin could be a novel, potential health-protective, natural agent against CAC.
35269806	11	73	dep	novel	2137:2141	arg1	health-protective					2154:2170	health-protective	2154:2170	health-protective	2154:2170	Therefore, corylin could be a novel, potential health-protective, natural agent against CAC.
35269806	4	74	theme	antitumor	721:729	arg1	effects					731:737	the possible antitumor effects	708:737	the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model	708:824	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	10	75	theme	colitis-associated	1983:2000	arg1	cancer					2008:2013	colitis-associated colon cancer	1983:2013	colitis-associated colon cancer	1983:2013	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	0	76	theme	Corylin	122:128	arg1	Effect					112:117	Anti-Inflammatory Effect	94:117	Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice	94:165	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	1	77	theme	colorectal	380:389	arg1	CAC					399:401	CAC	399:401	CAC	399:401	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	1	77	theme	colorectal	380:389	arg1	cancer					391:396	colitis-associated colorectal cancer	361:396	colitis-associated colorectal cancer (CAC)	361:402	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	5	78	theme	macrophage	894:903	arg1	polarization					905:916	macrophage polarization	894:916	macrophage polarization (CD11c, CCR7, CD163, and CD206)	894:948	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	11	79	theme	novel	2137:2141	arg1	agent					2181:2185	a novel, potential health-protective, natural agent	2135:2185	a novel, potential health-protective, natural agent against CAC	2135:2197	Therefore, corylin could be a novel, potential health-protective, natural agent against CAC.
35269806	11	79	theme	novel	2137:2141	arg1	corylin					2118:2124	corylin	2118:2124	corylin	2118:2124	Therefore, corylin could be a novel, potential health-protective, natural agent against CAC.
35269806	0	80	theme	Microbiota	18:27	arg1	Modulation					0:9	Modulation	0:9	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction	0:83	Modulation of Gut Microbiota Combined with Upregulation of Intestinal Tight Junction Explains Anti-Inflammatory Effect of Corylin on Colitis-Associated Cancer in Mice.
35269806	8	81	theme	Firmicutes/Bacteroidetes	1451:1474	arg1	ratio					1476:1480	the Firmicutes/Bacteroidetes ratio	1447:1480	the Firmicutes/Bacteroidetes ratio	1447:1480	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	5	82	theme	microbiota	955:964	arg1	dysbiosis					966:974	microbiota dysbiosis	955:974	microbiota dysbiosis	955:974	Biomarkers associated with inflammation, the colon tissue barrier, macrophage polarization (CD11c, CCR7, CD163, and CD206), and microbiota dysbiosis were monitored in the AOM/DSS group versus corylin groups.
35269806	2	83	theme	corylin	448:454	arg1	effects					437:443	the effects	433:443	the effects of corylin in a mouse model of dextran sodium sulfate (DSS)-induced colitis	433:519	In this study, we evaluated the effects of corylin in a mouse model of dextran sodium sulfate (DSS)-induced colitis.
35269806	8	84	theme	community	1549:1557	arg1	richness					1559:1566	community richness	1549:1566	community richness	1549:1566	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	6	85	theme	cytokines	1074:1082	arg1	expression					1121:1130	pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression	1057:1130	pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression	1057:1130	Corylin downregulated pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, and IL-6) mRNA expression and inflammatory signaling-associated markers (TLR4, MyD88, AP-1, CD11b, and F4/80).
35269806	10	86	theme	inflammation	2033:2044	arg1	regulation					2019:2028	regulation	2019:2028	regulation of inflammation, carcinogenesis, and compositional change of gut microbiota	2019:2104	Altogether, corylin administration showed cancer-ameliorating effects by reducing the risk of colitis-associated colon cancer via regulation of inflammation, carcinogenesis, and compositional change of gut microbiota.
35269806	8	87	theme	corylin	1495:1501	arg1	intervention					1503:1514	corylin intervention	1495:1514	corylin intervention	1495:1514	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	4	88	theme	corylin	765:771	arg1	treatment					752:760	30-day treatment	745:760	30-day treatment of corylin	745:771	Then, we further identified the possible antitumor effects after 30-day treatment of corylin on an azoxymethane (AOM)/DSS-induced CAC mouse model.
35269806	8	89	theme	AOM/DSS	1575:1581	arg1	mice					1583:1586	the AOM/DSS mice	1571:1586	the AOM/DSS mice	1571:1586	Furthermore, the Firmicutes/Bacteroidetes ratio changed with corylin intervention, and the microbial diversity and community richness of the AOM/DSS mice were improved by corylin.
35269806	1	90	theme	integrity	251:259	arg1	loss					232:235	loss	232:235	loss of epithelial integrity	232:259	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	1	90	theme	integrity	251:259	arg1	inflammation					218:229	chronic inflammation	210:229	chronic inflammation	210:229	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
35269806	1	90	theme	integrity	251:259	arg1	dysbiosis					294:302	gastrointestinal microbiota dysbiosis	266:302	gastrointestinal microbiota dysbiosis	266:302	Inflammatory bowel disease (IBD) involves chronic inflammation, loss of epithelial integrity, and gastrointestinal microbiota dysbiosis, resulting in the development of a colon cancer known as colitis-associated colorectal cancer (CAC).
37243079	4	0	theme	current	620:626	arg1	practices					647:655	current good manufacturing practices	620:655	current good manufacturing practices	620:655	A battery of analytical methods was developed in accordance with current good manufacturing practices to ensure a quality biopharmaceutical.
37243079	6	1	theme	MALDI-TOF	982:990	arg1	spectrometry					997:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	6	1	theme	MALDI-TOF	982:990	arg1	platform					1040:1047	a multi-attribute platform	1022:1047	a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification	1022:1125	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	0	2	theme	Vaccine	67:73	arg1	Candidate					75:83	an HIV-1 Envelope Glycoprotein Vaccine Candidate	36:83	an HIV-1 Envelope Glycoprotein Vaccine Candidate	36:83	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	0	2	theme	Vaccine	67:73	arg1	gp145					29:33	gp145	29:33	gp145	29:33	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	1	3	theme	promising	303:311	arg1	candidate					321:329	a promising vaccine candidate	301:329	a promising vaccine candidate	301:329	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	5	4	theme	circular	868:875	arg1	dichroism					877:885	circular dichroism	868:885	circular dichroism	868:885	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	1	5	theme	vaccine	313:319	arg1	candidate					321:329	a promising vaccine candidate	301:329	a promising vaccine candidate	301:329	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	9	6	from	use	1539:1541	arg1	trials					1578:1583	future preclinical and clinical trials	1546:1583	future preclinical and clinical trials	1546:1583	The unique properties of our gp145 microparticle make it amenable to use in future preclinical and clinical trials.
37243079	3	7	dep	pH	429:430	arg1	to					432:433	to	432:433	to	432:433	By adjusting the pH to 6.8, we increased expression levels to 101 mg/L in a 50 L bioreactor, nearly twice the previously reported titer value.
37243079	9	8	theme	preclinical	1553:1563	arg1	trials					1578:1583	future preclinical and clinical trials	1546:1583	future preclinical and clinical trials	1546:1583	The unique properties of our gp145 microparticle make it amenable to use in future preclinical and clinical trials.
37243079	7	9	theme	accurate	1257:1264	arg1	characterization					1266:1281	accurate characterization	1257:1281	accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine	1257:1361	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	1	10	theme	candidate	321:329	arg1	production					287:296	the production	283:296	the production of a promising vaccine candidate	283:329	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	5	11	dep	binding	948:954	arg1	i.e.					933:936	i.e.	933:936	i.e.	933:936	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	6	12	theme	glycans	1082:1088	arg1	analysis					1090:1097	glycans analysis	1082:1097	glycans analysis	1082:1097	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	1	13	theme	Chinese	240:246	arg1	cells					262:266	Chinese hamster ovary cells	240:266	Chinese hamster ovary cells	240:266	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	5	14	theme	dichroism	877:885	arg1	analysis					887:894	and bio-layer interferometry and circular dichroism analysis	835:894	and bio-layer interferometry and circular dichroism analysis	835:894	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	1	15	from	cells	262:266	arg1	gp145					229:233	HIV envelope protein (Env) gp145	202:233	HIV envelope protein (Env) gp145 from Chinese hamster ovary cells	202:266	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	6	16	theme	multi-attribute	1024:1038	arg1	spectrometry					997:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	6	16	theme	multi-attribute	1024:1038	arg1	platform					1040:1047	a multi-attribute platform	1022:1047	a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification	1022:1125	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	8	17	dep	gp145	1421:1425	arg1	encapsulated					1427:1438	encapsulated	1427:1438	encapsulated	1427:1438	Finally, we present a novel guanosine microparticle with gp145 encapsulated and displayed on its surface.
37243079	8	17	dep	gp145	1421:1425	arg1	displayed					1444:1452	displayed	1444:1452	displayed on its surface	1444:1467	Finally, we present a novel guanosine microparticle with gp145 encapsulated and displayed on its surface.
37243079	7	18	theme	effective	1345:1353	arg1	vaccine					1355:1361	an effective vaccine	1342:1361	an effective vaccine	1342:1361	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	4	19	theme	methods	579:585	arg1	battery					557:563	A battery	555:563	A battery of analytical methods	555:585	A battery of analytical methods was developed in accordance with current good manufacturing practices to ensure a quality biopharmaceutical.
37243079	5	20	theme	gp145	767:771	arg1	glycosylation					750:762	proper glycosylation	743:762	proper glycosylation of gp145	743:771	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	4	21	theme	analytical	568:577	arg1	methods					579:585	analytical methods	568:585	analytical methods	568:585	A battery of analytical methods was developed in accordance with current good manufacturing practices to ensure a quality biopharmaceutical.
37243079	7	22	theme	gp145	1170:1174	arg1	product					1176:1182	our gp145 product	1166:1182	our gp145 product	1166:1182	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	7	23	theme	standard	1215:1222	arg1	reference					1205:1213	a reference standard	1203:1222	a reference standard	1203:1222	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	3	24	theme	L	491:491	arg1	bioreactor					493:502	a 50 L bioreactor	486:502	a 50 L bioreactor	486:502	By adjusting the pH to 6.8, we increased expression levels to 101 mg/L in a 50 L bioreactor, nearly twice the previously reported titer value.
37243079	7	25	theme	characterization	1266:1281	arg1	importance					1243:1252	the importance	1239:1252	the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine	1239:1361	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	6	26	theme	accurate	1053:1060	arg1	determination					1067:1079	accurate mass determination	1053:1079	accurate mass determination	1053:1079	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	2	27	theme	growth	393:398	arg1	conditions					400:409	the growth conditions	389:409	the growth conditions	389:409	First in shake flasks, then in bioreactors, we optimized the growth conditions.
37243079	1	28	theme	hamster	248:254	arg1	cells					262:266	Chinese hamster ovary cells	240:266	Chinese hamster ovary cells	240:266	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	6	29	used	used	1014:1017	arg2	spectrometry					997:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	6	29	used	used	1014:1017	arg2	platform					1040:1047	a multi-attribute platform	1022:1047	a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification	1022:1125	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	5	30	theme	trimeric	813:820	arg1	arrangement					822:832	the trimeric arrangement	809:832	the trimeric arrangement	809:832	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	2	31	theme	shake	341:345	arg1	flasks					347:352	shake flasks	341:352	shake flasks	341:352	First in shake flasks, then in bioreactors, we optimized the growth conditions.
37243079	1	32	theme	ovary	256:260	arg1	cells					262:266	Chinese hamster ovary cells	240:266	Chinese hamster ovary cells	240:266	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	5	33	theme	proper	743:748	arg1	glycosylation					750:762	proper glycosylation	743:762	proper glycosylation of gp145	743:771	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	5	34	theme	light	782:786	arg1	scattering					788:797	dynamic light scattering	774:797	dynamic light scattering	774:797	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	5	35	theme	dynamic	774:780	arg1	scattering					788:797	dynamic light scattering	774:797	dynamic light scattering	774:797	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	0	36	theme	Guanosine	110:118	arg1	Microparticles					120:133	Guanosine Microparticles	110:133	Guanosine Microparticles	110:133	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	0	37	from	Candidate	75:83	arg1	Microparticles					120:133	Guanosine Microparticles	110:133	Guanosine Microparticles	110:133	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	4	38	theme	quality	669:675	arg1	biopharmaceutical					677:693	a quality biopharmaceutical	667:693	a quality biopharmaceutical	667:693	A battery of analytical methods was developed in accordance with current good manufacturing practices to ensure a quality biopharmaceutical.
37243079	5	39	theme	bio-layer	839:847	arg1	interferometry					849:862	bio-layer interferometry	839:862	bio-layer interferometry	839:862	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	9	40	theme	gp145	1499:1503	arg1	microparticle					1505:1517	our gp145 microparticle	1495:1517	our gp145 microparticle	1495:1517	The unique properties of our gp145 microparticle make it amenable to use in future preclinical and clinical trials.
37243079	8	41	theme	guanosine	1392:1400	arg1	microparticle					1402:1414	a novel guanosine microparticle	1384:1414	a novel guanosine microparticle with gp145 encapsulated and displayed on its surface	1384:1467	Finally, we present a novel guanosine microparticle with gp145 encapsulated and displayed on its surface.
37243079	0	42	theme	gp145	29:33	arg1	Production					15:24	the Production	11:24	the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles	11:133	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	8	43	theme	novel	1386:1390	arg1	microparticle					1402:1414	a novel guanosine microparticle	1384:1414	a novel guanosine microparticle with gp145 encapsulated and displayed on its surface	1384:1467	Finally, we present a novel guanosine microparticle with gp145 encapsulated and displayed on its surface.
37243079	1	44	theme	HIV	202:204	arg1	Env					224:226	Env	224:226	Env	224:226	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	1	44	theme	HIV	202:204	arg1	protein					215:221	HIV envelope protein	202:221	HIV envelope protein (Env) gp145 from Chinese hamster ovary cells	202:266	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	0	45	theme	HIV-1	39:43	arg1	Glycoprotein					54:65	HIV-1 Envelope Glycoprotein	39:65	an HIV-1 Envelope Glycoprotein Vaccine Candidate	36:83	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	9	46	theme	clinical	1569:1576	arg1	trials					1578:1583	future preclinical and clinical trials	1546:1583	future preclinical and clinical trials	1546:1583	The unique properties of our gp145 microparticle make it amenable to use in future preclinical and clinical trials.
37243079	8	47	with	microparticle	1402:1414	arg1	gp145					1421:1425	gp145	1421:1425	gp145 encapsulated and displayed on its surface	1421:1467	Finally, we present a novel guanosine microparticle with gp145 encapsulated and displayed on its surface.
37243079	4	48	theme	manufacturing	633:645	arg1	practices					647:655	current good manufacturing practices	620:655	current good manufacturing practices	620:655	A battery of analytical methods was developed in accordance with current good manufacturing practices to ensure a quality biopharmaceutical.
37243079	7	49	theme	robust	1132:1137	arg1	analysis					1139:1146	Our robust analysis	1128:1146	Our robust analysis	1128:1146	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	9	50	theme	microparticle	1505:1517	arg1	properties					1481:1490	The unique properties	1470:1490	The unique properties of our gp145 microparticle	1470:1517	The unique properties of our gp145 microparticle make it amenable to use in future preclinical and clinical trials.
37243079	9	51	theme	unique	1474:1479	arg1	properties					1481:1490	The unique properties	1470:1490	The unique properties of our gp145 microparticle	1470:1517	The unique properties of our gp145 microparticle make it amenable to use in future preclinical and clinical trials.
37243079	4	52	theme	good	628:631	arg1	practices					647:655	current good manufacturing practices	620:655	current good manufacturing practices	620:655	A battery of analytical methods was developed in accordance with current good manufacturing practices to ensure a quality biopharmaceutical.
37243079	7	53	theme	vaccine	1355:1361	arg1	development					1327:1337	the development	1323:1337	the development of an effective vaccine	1323:1361	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	0	54	from	Encapsulation	93:105	arg1	Microparticles					120:133	Guanosine Microparticles	110:133	Guanosine Microparticles	110:133	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	3	55	theme	titer	542:546	arg1	value					548:552	the previously reported titer value	518:552	the previously reported titer value	518:552	By adjusting the pH to 6.8, we increased expression levels to 101 mg/L in a 50 L bioreactor, nearly twice the previously reported titer value.
37243079	6	56	theme	protein	1104:1110	arg1	identification					1112:1125	protein identification	1104:1125	protein identification	1104:1125	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	1	57	theme	envelope	206:213	arg1	Env					224:226	Env	224:226	Env	224:226	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	1	57	theme	envelope	206:213	arg1	protein					215:221	HIV envelope protein	202:221	HIV envelope protein (Env) gp145 from Chinese hamster ovary cells	202:266	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	5	58	theme	secondary	960:968	arg1	structure					970:978	secondary structure	960:978	secondary structure	960:978	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	5	59	theme	antibody	939:946	arg1	binding					948:954	antibody binding	939:954	antibody binding	939:954	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	5	60	theme	native-like	909:919	arg1	properties					921:930	native-like properties	909:930	native-like properties (i.e., antibody binding and secondary structure)	909:979	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	1	61	theme	protein	215:221	arg1	gp145					229:233	HIV envelope protein (Env) gp145	202:233	HIV envelope protein (Env) gp145 from Chinese hamster ovary cells	202:266	We have developed a pipeline to express, purify, and characterize HIV envelope protein (Env) gp145 from Chinese hamster ovary cells, to accelerate the production of a promising vaccine candidate.
37243079	0	62	theme	Glycoprotein	54:65	arg1	Candidate					75:83	an HIV-1 Envelope Glycoprotein Vaccine Candidate	36:83	an HIV-1 Envelope Glycoprotein Vaccine Candidate	36:83	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	0	62	theme	Glycoprotein	54:65	arg1	gp145					29:33	gp145	29:33	gp145	29:33	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	7	63	theme	heterogeneous	1295:1307	arg1	immunogen					1309:1317	a highly heterogeneous immunogen	1286:1317	a highly heterogeneous immunogen for the development of an effective vaccine	1286:1361	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	5	64	gly	glycosylation	750:762	arg1	gp145					767:771	gp145	767:771	gp145	767:771	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	7	65	theme	immunogen	1309:1317	arg1	characterization					1266:1281	accurate characterization	1257:1281	accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine	1257:1361	Our robust analysis demonstrates that our gp145 product is very similar to a reference standard and emphasizes the importance of accurate characterization of a highly heterogeneous immunogen for the development of an effective vaccine.
37243079	3	66	theme	expression	453:462	arg1	levels					464:469	expression levels	453:469	expression levels	453:469	By adjusting the pH to 6.8, we increased expression levels to 101 mg/L in a 50 L bioreactor, nearly twice the previously reported titer value.
37243079	6	67	theme	mass	1062:1065	arg1	determination					1067:1079	accurate mass determination	1053:1079	accurate mass determination	1053:1079	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	9	68	theme	future	1546:1551	arg1	trials					1578:1583	future preclinical and clinical trials	1546:1583	future preclinical and clinical trials	1546:1583	The unique properties of our gp145 microparticle make it amenable to use in future preclinical and clinical trials.
37243079	0	69	theme	Envelope	45:52	arg1	Glycoprotein					54:65	HIV-1 Envelope Glycoprotein	39:65	an HIV-1 Envelope Glycoprotein Vaccine Candidate	36:83	Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsulation in Guanosine Microparticles.
37243079	3	70	theme	reported	533:540	arg1	value					548:552	the previously reported titer value	518:552	the previously reported titer value	518:552	By adjusting the pH to 6.8, we increased expression levels to 101 mg/L in a 50 L bioreactor, nearly twice the previously reported titer value.
37243079	5	71	theme	interferometry	849:862	arg1	analysis					887:894	and bio-layer interferometry and circular dichroism analysis	835:894	and bio-layer interferometry and circular dichroism analysis	835:894	Imaged capillary isoelectric focusing verified proper glycosylation of gp145; dynamic light scattering confirmed the trimeric arrangement; and bio-layer interferometry and circular dichroism analysis demonstrated native-like properties (i.e., antibody binding and secondary structure).
37243079	6	72	theme	mass	992:995	arg1	spectrometry					997:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry	982:1008	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
37243079	6	72	theme	mass	992:995	arg1	platform					1040:1047	a multi-attribute platform	1022:1047	a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification	1022:1125	MALDI-TOF mass spectrometry was used as a multi-attribute platform for accurate mass determination, glycans analysis, and protein identification.
35949121	2	0	theme	different	653:661	arg1	types					663:667	different types	653:667	different types of lipopolysaccharide	653:689	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	2	1	from	emphasis	608:615	arg1	characteristics					634:648	the molecular characteristics	620:648	the molecular characteristics of different types of lipopolysaccharide	620:689	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	5	2	theme	ischemia-reperfusion	1459:1478	arg1	model					1491:1495	ischemia-reperfusion arrhythmia model	1459:1495	ischemia-reperfusion arrhythmia model	1459:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	3	theme	diphosphoryl	1129:1140	arg1	residue					1142:1148	diphosphoryl residue	1129:1148	diphosphoryl residue	1129:1148	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	3	4	theme	A	796:796	arg1	effect					765:770	the antiarrhythmic effect	746:770	the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide	746:827	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	1	5	theme	minnesota	283:291	arg1	lipopolysaccharide					293:310	Salmonella minnesota lipopolysaccharide	272:310	Salmonella minnesota lipopolysaccharide	272:310	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	5	6	dep	time	1543:1546	arg1	n=6					1708:1710	n=6	1708:1710	n=6	1708:1710	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	6	dep	time	1543:1546	arg1	e.g.					1531:1534	e.g.	1531:1534	e.g.	1531:1534	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	6	dep	time	1543:1546	arg1	5.0					1591:1593	saline 25.3 5.0	1579:1593	saline 25.3 5.0	1579:1593	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	6	dep	time	1543:1546	arg1	±					1628:1628	E. coli lipopolysaccharide 24.3 ± 7.1	1596:1632	E. coli lipopolysaccharide 24.3 ± 7.1	1596:1632	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	6	dep	time	1543:1546	arg1	>					1715:1715	P > .05	1713:1719	P > .05	1713:1719	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	3	7	theme	monophosphoryl	775:788	arg1	A					796:796	monophosphoryl lipid A	775:796	monophosphoryl lipid A	775:796	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	5	8	theme	lipid	1442:1446	arg1	A					1448:1448	monophosphoryl lipid A Re595	1427:1454	monophosphoryl lipid A Re595	1427:1454	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	9	theme	typhimurium	940:950	arg1	684					955:957	Salmonella typhimurium SL 684	929:957	Salmonella typhimurium SL 684	929:957	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	3	10	theme	lipopolysaccharide	810:827	arg1	effect					765:770	the antiarrhythmic effect	746:770	the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide	746:827	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	5	11	theme	aconitine-	1519:1528	arg1	models					1752:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	12	theme	ouabain-induced	1725:1739	arg1	models					1752:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	13	theme	ventricular	1174:1184	arg1	tachycardia					1186:1196	ventricular tachycardia	1174:1196	ventricular tachycardia	1174:1196	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	5	14	theme	monophosphoryl	1656:1669	arg1	A					1677:1677	monophosphoryl lipid A SL684	1656:1683	monophosphoryl lipid A SL684 23.8 ± 4.3	1656:1694	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	14	theme	monophosphoryl	1656:1669	arg1	±					1649:1649	24.0 ± 4.5	1644:1653	24.0 ± 4.5	1644:1653	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	3	15	theme	E.	802:803	arg1	lipopolysaccharide					810:827	E. coli lipopolysaccharide	802:827	E. coli lipopolysaccharide	802:827	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	1	16	theme	monophosphoryl	213:226	arg1	derivative					258:267	a nonpyrogenic derivative	243:267	a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide	243:310	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	16	theme	monophosphoryl	213:226	arg1	A					234:234	monophosphoryl lipid A Re595	213:240	monophosphoryl lipid A Re595	213:240	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	4	17	theme	monophosphoryl	1015:1028	arg1	A					1036:1036	monophosphoryl lipid A Re595	1015:1042	monophosphoryl lipid A Re595	1015:1042	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	1	18	theme	A	234:234	arg1	doses					152:156	sublethal doses	142:156	sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide,	142:311	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	4	19	theme	monophosphoryl	974:987	arg1	residue					989:995	only monophosphoryl residue	969:995	only monophosphoryl residue in its structure	969:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	6	20	theme	subcomponent	1975:1986	arg1	composition					1988:1998	its subcomponent composition	1971:1998	its subcomponent composition for this antiarrhythmic effect	1971:2029	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	4	21	dep	tachycardia	1186:1196	arg1	<					1312:1312	P < .05	1310:1316	P < .05	1310:1316	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	22	theme	monophosphoryl	1246:1259	arg1	A					1267:1267	monophosphoryl lipid A Re595	1246:1273	monophosphoryl lipid A Re595	1246:1273	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	1	23	theme	sublethal	142:150	arg1	doses					152:156	sublethal doses	142:156	sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide,	142:311	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	2	24	theme	-induced	537:544	arg1	models					557:562	drug (aconitine or ouabain)-induced arrhythmia models	510:562	drug (aconitine or ouabain)-induced arrhythmia models	510:562	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	5	25	theme	lipid	1671:1675	arg1	A					1677:1677	monophosphoryl lipid A SL684	1656:1683	monophosphoryl lipid A SL684 23.8 ± 4.3	1656:1694	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	25	theme	lipid	1671:1675	arg1	±					1649:1649	24.0 ± 4.5	1644:1653	24.0 ± 4.5	1644:1653	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	6	26	theme	diphosphoryl	1948:1959	arg1	groups					1961:1966	diphosphoryl groups	1948:1966	diphosphoryl groups	1948:1966	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	3	27	theme	arrhythmia	868:877	arg1	model					879:883	the ischemia-reperfusion arrhythmia model	843:883	the ischemia-reperfusion arrhythmia model	843:883	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	3	28	dep	E.	802:803	arg1	coli					805:808	coli	805:808	coli	805:808	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	0	29	theme	Arrhythmia	74:83	arg1	Models					56:61	Rodent Models	49:61	Rodent Models of Cardiac Arrhythmia	49:83	The Effects of Lipopolysaccharide Derivatives in Rodent Models of Cardiac Arrhythmia.
35949121	1	30	theme	ischemia-reperfusion	364:383	arg1	arrhythmias					385:395	ischemia-reperfusion arrhythmias	364:395	ischemia-reperfusion arrhythmias	364:395	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	31	theme	coli	173:176	arg1	endotoxin					198:206	endotoxin	198:206	endotoxin	198:206	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	31	theme	coli	173:176	arg1	lipopolysaccharide					178:195	Escherichia coli lipopolysaccharide	161:195	Escherichia coli lipopolysaccharide (endotoxin)	161:207	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	2	32	theme	protective	425:434	arg1	effect					436:441	the protective effect	421:441	the protective effect of lipopolysaccharide derivatives	421:475	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	6	33	theme	antiarrhythmic	2009:2022	arg1	effect					2024:2029	this antiarrhythmic effect	2004:2029	this antiarrhythmic effect	2004:2029	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	1	34	theme	lipopolysaccharide	293:310	arg1	derivative					258:267	a nonpyrogenic derivative	243:267	a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide	243:310	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	34	theme	lipopolysaccharide	293:310	arg1	A					234:234	monophosphoryl lipid A Re595	213:240	monophosphoryl lipid A Re595	213:240	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	35	theme	Several	97:103	arg1	studies					114:120	BACKGROUND Several previous studies	86:120	BACKGROUND Several previous studies	86:120	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	5	36	from	effects	1385:1391	arg1	model					1491:1495	ischemia-reperfusion arrhythmia model	1459:1495	ischemia-reperfusion arrhythmia model	1459:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	2	37	theme	derivatives	465:475	arg1	effect					436:441	the protective effect	421:441	the protective effect of lipopolysaccharide derivatives	421:475	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	5	38	theme	saline	1579:1584	arg1	5.0					1591:1593	saline 25.3 5.0	1579:1593	saline 25.3 5.0	1579:1593	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	39	theme	P	1310:1310	arg1	<					1312:1312	P < .05	1310:1316	P < .05	1310:1316	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	0	40	theme	Derivatives	34:44	arg1	Effects					4:10	The Effects	0:10	The Effects of Lipopolysaccharide Derivatives in Rodent Models of Cardiac Arrhythmia	0:83	The Effects of Lipopolysaccharide Derivatives in Rodent Models of Cardiac Arrhythmia.
35949121	4	41	theme	coli	1080:1083	arg1	lipopolysaccharide					1085:1102	E. coli lipopolysaccharide	1077:1102	E. coli lipopolysaccharide which have both mono and diphosphoryl residue	1077:1148	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	42	theme	lipid	916:920	arg1	A					922:922	monophosphoryl lipid A	901:922	monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure	901:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	5	43	theme	lipopolysaccharide	1404:1421	arg1	absent					1502:1507	absent	1502:1507	absent	1502:1507	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	43	theme	lipopolysaccharide	1404:1421	arg1	effects					1385:1391	The antiarrhythmic effects	1366:1391	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model	1366:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	44	theme	P	1713:1713	arg1	>					1715:1715	P > .05	1713:1719	P > .05	1713:1719	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	0	45	theme	Rodent	49:54	arg1	Models					56:61	Rodent Models	49:61	Rodent Models of Cardiac Arrhythmia	49:83	The Effects of Lipopolysaccharide Derivatives in Rodent Models of Cardiac Arrhythmia.
35949121	5	46	theme	ventricular	1552:1562	arg1	beats					1572:1576	ventricular ectopic beats	1552:1576	ventricular ectopic beats	1552:1576	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	47	contain	have	1110:1113	arg2	mono					1120:1123	mono	1120:1123	mono	1120:1123	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	47	contain	have	1110:1113	arg2	residue					1142:1148	diphosphoryl residue	1129:1148	diphosphoryl residue	1129:1148	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	47	contain	have	1110:1113	arg1	lipopolysaccharide					1085:1102	E. coli lipopolysaccharide	1077:1102	E. coli lipopolysaccharide which have both mono and diphosphoryl residue	1077:1148	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	5	48	theme	monophosphoryl	1427:1440	arg1	A					1448:1448	monophosphoryl lipid A Re595	1427:1454	monophosphoryl lipid A Re595	1427:1454	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	3	49	dep	RESULTS	692:698	arg1	tested					833:838	tested	833:838	was tested in the ischemia-reperfusion arrhythmia model	829:883	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	6	50	contain	possess	1940:1946	arg1	lipopolysaccharide					1914:1931	lipopolysaccharide	1914:1931	lipopolysaccharide	1914:1931	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	6	50	contain	possess	1940:1946	arg2	groups					1961:1966	diphosphoryl groups	1948:1966	diphosphoryl groups	1948:1966	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	2	51	from	conclusions	569:579	arg1	study					414:418	this study	409:418	this study	409:418	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	5	52	theme	lipopolysaccharide	1604:1621	arg1	n=6					1708:1710	n=6	1708:1710	n=6	1708:1710	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	52	theme	lipopolysaccharide	1604:1621	arg1	±					1628:1628	E. coli lipopolysaccharide 24.3 ± 7.1	1596:1632	E. coli lipopolysaccharide 24.3 ± 7.1	1596:1632	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	53	theme	n=8-10	1302:1307	arg1	reperfusion					1212:1222	reperfusion	1212:1222	reperfusion	1212:1222	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	54	theme	ventricular	1340:1350	arg1	fibrillation					1352:1363	ventricular fibrillation	1340:1363	ventricular fibrillation	1340:1363	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	5	55	theme	E.	1396:1397	arg1	lipopolysaccharide					1404:1421	E. coli lipopolysaccharide	1396:1421	E. coli lipopolysaccharide	1396:1421	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	56	theme	SL	952:953	arg1	684					955:957	Salmonella typhimurium SL 684	929:957	Salmonella typhimurium SL 684	929:957	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	3	57	theme	antiarrhythmic	750:763	arg1	effect					765:770	the antiarrhythmic effect	746:770	the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide	746:827	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	5	58	theme	arrhythmia	1480:1489	arg1	model					1491:1495	ischemia-reperfusion arrhythmia model	1459:1495	ischemia-reperfusion arrhythmia model	1459:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	1	59	theme	Salmonella	272:281	arg1	lipopolysaccharide					293:310	Salmonella minnesota lipopolysaccharide	272:310	Salmonella minnesota lipopolysaccharide	272:310	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	2	60	theme	molecular	624:632	arg1	characteristics					634:648	the molecular characteristics	620:648	the molecular characteristics of different types of lipopolysaccharide	620:689	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	5	61	theme	E.	1596:1597	arg1	n=6					1708:1710	n=6	1708:1710	n=6	1708:1710	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	61	theme	E.	1596:1597	arg1	±					1628:1628	E. coli lipopolysaccharide 24.3 ± 7.1	1596:1632	E. coli lipopolysaccharide 24.3 ± 7.1	1596:1632	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	1	62	theme	lipopolysaccharide	178:195	arg1	doses					152:156	sublethal doses	142:156	sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide,	142:311	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	2	63	theme	lipopolysaccharide	672:689	arg1	types					663:667	different types	653:667	different types of lipopolysaccharide	653:689	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	2	64	from	METHODS	398:404	arg1	study					414:418	this study	409:418	this study	409:418	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	3	65	theme	lipid	790:794	arg1	A					796:796	monophosphoryl lipid A	775:796	monophosphoryl lipid A	775:796	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	5	66	theme	A	1448:1448	arg1	absent					1502:1507	absent	1502:1507	absent	1502:1507	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	66	theme	A	1448:1448	arg1	effects					1385:1391	The antiarrhythmic effects	1366:1391	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model	1366:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	67	from	684	955:957	arg1	A					922:922	monophosphoryl lipid A	901:922	monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure	901:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	2	68	theme	types	663:667	arg1	characteristics					634:648	the molecular characteristics	620:648	the molecular characteristics of different types of lipopolysaccharide	620:689	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	4	69	theme	tachycardia	1186:1196	arg1	incidence					1327:1335	the incidence	1323:1335	the incidence of ventricular fibrillation	1323:1363	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	69	theme	tachycardia	1186:1196	arg1	duration					1162:1169	the duration	1158:1169	the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05)	1158:1317	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	6	70	theme	ischemia-reperfusion	1876:1895	arg1	arrhythmias					1897:1907	ischemia-reperfusion arrhythmias	1876:1907	ischemia-reperfusion arrhythmias	1876:1907	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	5	71	theme	arrhythmia	1741:1750	arg1	models					1752:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	72	from	absent	1502:1507	arg1	mice					1762:1765	mice	1762:1765	mice	1762:1765	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	72	from	absent	1502:1507	arg1	models					1752:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models	1512:1757	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	1	73	theme	lipid	228:232	arg1	derivative					258:267	a nonpyrogenic derivative	243:267	a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide	243:310	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	73	theme	lipid	228:232	arg1	A					234:234	monophosphoryl lipid A Re595	213:240	monophosphoryl lipid A Re595	213:240	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	5	74	dep	aconitine-	1519:1528	arg1	time					1543:1546	onset time	1537:1546	onset time for ventricular ectopic beats	1537:1576	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	6	75	theme	antiarrhythmic	1846:1859	arg1	effect					1861:1866	antiarrhythmic effect	1846:1866	antiarrhythmic effect	1846:1866	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	3	76	theme	ischemia-reperfusion	847:866	arg1	model					879:883	the ischemia-reperfusion arrhythmia model	843:883	the ischemia-reperfusion arrhythmia model	843:883	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	4	77	theme	only	969:972	arg1	residue					989:995	only monophosphoryl residue	969:995	only monophosphoryl residue in its structure	969:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	78	theme	lipid	1261:1265	arg1	A					1267:1267	monophosphoryl lipid A Re595	1246:1273	monophosphoryl lipid A Re595	1246:1273	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	79	from	residue	989:995	arg1	structure					1004:1012	its structure	1000:1012	its structure	1000:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	5	80	from	models	1752:1757	arg1	absent					1502:1507	absent	1502:1507	absent	1502:1507	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	80	from	models	1752:1757	arg1	effects					1385:1391	The antiarrhythmic effects	1366:1391	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model	1366:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	2	81	theme	particular	597:606	arg1	emphasis					608:615	particular emphasis	597:615	particular emphasis on the molecular characteristics of different types of lipopolysaccharide	597:689	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	0	82	theme	Cardiac	66:72	arg1	Arrhythmia					74:83	Cardiac Arrhythmia	66:83	Cardiac Arrhythmia	66:83	The Effects of Lipopolysaccharide Derivatives in Rodent Models of Cardiac Arrhythmia.
35949121	4	83	dep	reperfusion	1212:1222	arg1	vehicle					1225:1231	vehicle	1225:1231	vehicle	1225:1231	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	83	dep	reperfusion	1212:1222	arg1	±					1238:1238	176 ± 22.8	1234:1243	176 ± 22.8	1234:1243	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	83	dep	reperfusion	1212:1222	arg1	A					1267:1267	monophosphoryl lipid A Re595	1246:1273	monophosphoryl lipid A Re595	1246:1273	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	83	dep	reperfusion	1212:1222	arg1	±					1283:1283	132.83 ± 12.1	1276:1288	132.83 ± 12.1	1276:1288	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	5	84	dep	A	1677:1677	arg1	±					1690:1690	23.8 ± 4.3	1685:1694	monophosphoryl lipid A SL684 23.8 ± 4.3	1656:1694	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	2	85	dep	drug	510:513	arg1	ouabain					529:535	ouabain	529:535	ouabain	529:535	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	2	85	dep	drug	510:513	arg1	aconitine					516:524	aconitine	516:524	aconitine	516:524	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	1	86	theme	Escherichia	161:171	arg1	endotoxin					198:206	endotoxin	198:206	endotoxin	198:206	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	86	theme	Escherichia	161:171	arg1	lipopolysaccharide					178:195	Escherichia coli lipopolysaccharide	161:195	Escherichia coli lipopolysaccharide (endotoxin)	161:207	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	4	87	contain	has	965:967	arg1	A					922:922	monophosphoryl lipid A	901:922	monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure	901:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	87	contain	has	965:967	arg2	residue					989:995	only monophosphoryl residue	969:995	only monophosphoryl residue in its structure	969:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	0	88	from	Effects	4:10	arg1	Models					56:61	Rodent Models	49:61	Rodent Models of Cardiac Arrhythmia	49:83	The Effects of Lipopolysaccharide Derivatives in Rodent Models of Cardiac Arrhythmia.
35949121	6	89	dep	CONCLUSION	1768:1777	arg1	conclude					1793:1800	conclude	1793:1800	conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect	1793:2029	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	1	90	theme	arrhythmias	385:395	arg1	model					355:359	the rat model	347:359	the rat model of ischemia-reperfusion arrhythmias	347:395	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	5	91	from	mice	1762:1765	arg1	absent					1502:1507	absent	1502:1507	absent	1502:1507	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	91	from	mice	1762:1765	arg1	effects					1385:1391	The antiarrhythmic effects	1366:1391	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model	1366:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	92	dep	<	1312:1312	arg1	e.g.					1199:1202	e.g.	1199:1202	e.g.	1199:1202	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	93	theme	lipid	1030:1034	arg1	A					1036:1036	monophosphoryl lipid A Re595	1015:1042	monophosphoryl lipid A Re595	1015:1042	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	5	94	theme	onset	1537:1541	arg1	time					1543:1546	onset time	1537:1546	onset time for ventricular ectopic beats	1537:1576	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	2	95	theme	arrhythmia	546:555	arg1	models					557:562	drug (aconitine or ouabain)-induced arrhythmia models	510:562	drug (aconitine or ouabain)-induced arrhythmia models	510:562	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	1	96	theme	BACKGROUND	86:95	arg1	studies					114:120	BACKGROUND Several previous studies	86:120	BACKGROUND Several previous studies	86:120	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	3	97	theme	molecular	722:730	arg1	structure					732:740	the molecular structure	718:740	the molecular structure	718:740	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	0	98	theme	Lipopolysaccharide	15:32	arg1	Derivatives					34:44	Lipopolysaccharide Derivatives	15:44	Lipopolysaccharide Derivatives	15:44	The Effects of Lipopolysaccharide Derivatives in Rodent Models of Cardiac Arrhythmia.
35949121	5	99	dep	±	1628:1628	arg1	vehicle					1635:1641	vehicle	1635:1641	vehicle	1635:1641	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	99	dep	±	1628:1628	arg1	±					1649:1649	24.0 ± 4.5	1644:1653	24.0 ± 4.5	1644:1653	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	99	dep	±	1628:1628	arg1	A					1677:1677	monophosphoryl lipid A SL684	1656:1683	monophosphoryl lipid A SL684 23.8 ± 4.3	1656:1694	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	1	100	theme	previous	105:112	arg1	studies					114:120	BACKGROUND Several previous studies	86:120	BACKGROUND Several previous studies	86:120	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	5	101	dep	E.	1396:1397	arg1	coli					1399:1402	coli	1399:1402	coli	1399:1402	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	2	102	theme	lipopolysaccharide	446:463	arg1	derivatives					465:475	lipopolysaccharide derivatives	446:475	lipopolysaccharide derivatives	446:475	METHODS In this study, the protective effect of lipopolysaccharide derivatives was also further investigated in drug (aconitine or ouabain)-induced arrhythmia models, and conclusions were drawn with particular emphasis on the molecular characteristics of different types of lipopolysaccharide.
35949121	1	103	theme	antiarrhythmic	321:334	arg1	effects					336:342	antiarrhythmic effects	321:342	antiarrhythmic effects	321:342	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	5	104	dep	E.	1596:1597	arg1	coli					1599:1602	coli	1599:1602	coli	1599:1602	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	4	105	theme	Salmonella	929:938	arg1	684					955:957	Salmonella typhimurium SL 684	929:957	Salmonella typhimurium SL 684	929:957	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	106	theme	E.	1077:1078	arg1	lipopolysaccharide					1085:1102	E. coli lipopolysaccharide	1077:1102	E. coli lipopolysaccharide which have both mono and diphosphoryl residue	1077:1148	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	3	107	theme	structure	732:740	arg1	importance					704:713	The importance	700:713	The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide	700:827	RESULTS The importance of the molecular structure for the antiarrhythmic effect of monophosphoryl lipid A and E. coli lipopolysaccharide was tested in the ischemia-reperfusion arrhythmia model.
35949121	4	108	theme	monophosphoryl	901:914	arg1	A					922:922	monophosphoryl lipid A	901:922	monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure	901:1012	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	1	109	theme	rat	351:353	arg1	model					355:359	the rat model	347:359	the rat model of ischemia-reperfusion arrhythmias	347:395	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	5	110	theme	ectopic	1564:1570	arg1	beats					1572:1576	ventricular ectopic beats	1552:1576	ventricular ectopic beats	1552:1576	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	111	theme	antiarrhythmic	1370:1383	arg1	absent					1502:1507	absent	1502:1507	absent	1502:1507	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	5	111	theme	antiarrhythmic	1370:1383	arg1	effects					1385:1391	The antiarrhythmic effects	1366:1391	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model	1366:1495	The antiarrhythmic effects of E. coli lipopolysaccharide and monophosphoryl lipid A Re595 in ischemia-reperfusion arrhythmia model were absent in either aconitine- (e.g., onset time for ventricular ectopic beats: saline 25.3 5.0, E. coli lipopolysaccharide 24.3 ± 7.1; vehicle: 24.0 ± 4.5, monophosphoryl lipid A SL684 23.8 ± 4.3, as second, n=6, P > .05) or ouabain-induced arrhythmia models in mice.
35949121	6	112	theme	lipopolysaccharide	1807:1824	arg1	derivatives					1826:1836	lipopolysaccharide derivatives	1807:1836	lipopolysaccharide derivatives	1807:1836	CONCLUSION Therefore, we conclude that lipopolysaccharide derivatives exhibit antiarrhythmic effect only in ischemia-reperfusion arrhythmias, and lipopolysaccharide should possess diphosphoryl groups in its subcomponent composition for this antiarrhythmic effect.
35949121	4	113	theme	fibrillation	1352:1363	arg1	incidence					1327:1335	the incidence	1323:1335	the incidence of ventricular fibrillation	1323:1363	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	4	113	theme	fibrillation	1352:1363	arg1	duration					1162:1169	the duration	1158:1169	the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05)	1158:1317	In contrast to monophosphoryl lipid A from Salmonella typhimurium SL 684 which has only monophosphoryl residue in its structure, monophosphoryl lipid A Re595, obtained from S. minnesota, and E. coli lipopolysaccharide which have both mono and diphosphoryl residue reduced the duration of ventricular tachycardia (e.g., during reperfusion: vehicle: 176 ± 22.8; monophosphoryl lipid A Re595: 132.83 ± 12.1, as second, n=8-10, P < .05) and the incidence of ventricular fibrillation.
35949121	1	114	theme	nonpyrogenic	245:256	arg1	derivative					258:267	a nonpyrogenic derivative	243:267	a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide	243:310	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
35949121	1	114	theme	nonpyrogenic	245:256	arg1	A					234:234	monophosphoryl lipid A Re595	213:240	monophosphoryl lipid A Re595	213:240	BACKGROUND Several previous studies have suggested that sublethal doses of Escherichia coli lipopolysaccharide (endotoxin) and monophosphoryl lipid A Re595, a nonpyrogenic derivative of Salmonella minnesota lipopolysaccharide, exhibit antiarrhythmic effects in the rat model of ischemia-reperfusion arrhythmias.
37375650	0	0	theme	Ulcerative	107:116	arg1	Colitis					118:124	Ulcerative Colitis	107:124	Ulcerative Colitis in Mice	107:132	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	0	1	from	Effect	15:20	arg1	Sulfate					84:90	Dextran Sulfate	76:90	Dextran Sulfate	76:90	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	5	2	dep	preventing	834:843	arg1	e.g.					828:831	e.g.	828:831	e.g.	828:831	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	3	from	increase	1162:1169	arg1	malondialdehyde					1194:1208	malondialdehyde	1194:1208	malondialdehyde	1194:1208	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	3	from	increase	1162:1169	arg1	myeloperoxidase					1174:1188	myeloperoxidase	1174:1188	myeloperoxidase	1174:1188	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	4	theme	length	912:917	arg1	increase					849:856	the increase	845:856	the increase in disease activity index	845:882	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	4	theme	length	912:917	arg1	shortening					892:901	the shortening	888:901	the shortening of colon length	888:917	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	4	5	theme	CC	627:628	arg1	effects					599:605	the interventional effects	580:605	the interventional effects of heat-inactivated CC (HICC) on UC mice	580:646	Therefore, the interventional effects of heat-inactivated CC (HICC) on UC mice were explored.
37375650	5	6	theme	pathologic	802:811	arg1	lesions					813:819	the pathologic lesions	798:819	the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length)	798:918	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	7	dep	attenuating	1106:1116	arg1	3					1103:1103	3	1103:1103	3	1103:1103	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	1	8	theme	postbiotics	190:200	arg1	class					181:185	a typical class	171:185	a typical class of postbiotics with promising potential health effects	171:240	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	1	8	theme	postbiotics	190:200	arg1	microorganisms					152:165	Heat-inactivated microorganisms	135:165	Heat-inactivated microorganisms	135:165	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	4	9	theme	heat-inactivated	610:625	arg1	HICC					631:634	HICC	631:634	HICC	631:634	Therefore, the interventional effects of heat-inactivated CC (HICC) on UC mice were explored.
37375650	4	9	theme	heat-inactivated	610:625	arg1	CC					627:628	heat-inactivated CC	610:628	CC	627:628	Therefore, the interventional effects of heat-inactivated CC (HICC) on UC mice were explored.
37375650	2	10	contain	have	391:394	arg2	potential					400:408	the potential to alleviate ulcerative colitis (UC)	396:445	the potential to alleviate ulcerative colitis (UC)	396:445	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	2	10	contain	have	391:394	arg1	supplementation					310:324	Dietary supplementation	302:324	Dietary supplementation with Companilactobacillus crustorum MN047 (CC)	302:371	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	5	11	theme	colon	906:910	arg1	length					912:917	colon length	906:917	colon length	906:917	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	1	12	with	class	181:185	arg1	effects					234:240	promising potential health effects	207:240	promising potential health effects	207:240	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	5	13	theme	barrier	1245:1251	arg1	damage					1231:1236	the damage	1227:1236	the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels)	1227:1312	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	6	14	contain	has	1548:1550	arg2	potential					1556:1564	the potential	1552:1564	the potential as a dietary supplement to intervene in UC	1552:1607	In conclusion, our study suggested that HICC can be effective in preventing UC and has the potential as a dietary supplement to intervene in UC.
37375650	6	14	contain	has	1548:1550	arg1	HICC					1505:1508	HICC	1505:1508	HICC	1505:1508	In conclusion, our study suggested that HICC can be effective in preventing UC and has the potential as a dietary supplement to intervene in UC.
37375650	5	15	theme	gut	1334:1336	arg1	structure					1349:1357	gut microbiota structure	1334:1357	gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus)	1334:1462	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	2	16	theme	Companilactobacillus	331:350	arg1	CC					369:370	CC	369:370	CC	369:370	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	2	16	theme	Companilactobacillus	331:350	arg1	MN047					362:366	Companilactobacillus crustorum MN047	331:366	Companilactobacillus crustorum MN047 (CC)	331:371	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	6	17	theme	dietary	1571:1577	arg1	supplement					1579:1588	a dietary supplement	1569:1588	a dietary supplement	1569:1588	In conclusion, our study suggested that HICC can be effective in preventing UC and has the potential as a dietary supplement to intervene in UC.
37375650	5	18	theme	microbiota	1338:1347	arg1	structure					1349:1357	gut microbiota structure	1334:1357	gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus)	1334:1462	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	1	19	theme	promising	207:215	arg1	effects					234:240	promising potential health effects	207:240	promising potential health effects	207:240	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	0	20	theme	Protective	4:13	arg1	Effect					15:20	The Protective Effect	0:20	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate	0:90	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	1	21	theme	potential	217:225	arg1	effects					234:240	promising potential health effects	207:240	promising potential health effects	207:240	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	4	22	from	effects	599:605	arg1	mice					643:646	UC mice	640:646	UC mice	640:646	Therefore, the interventional effects of heat-inactivated CC (HICC) on UC mice were explored.
37375650	4	23	theme	interventional	584:597	arg1	effects					599:605	the interventional effects	580:605	the interventional effects of heat-inactivated CC (HICC) on UC mice	580:646	Therefore, the interventional effects of heat-inactivated CC (HICC) on UC mice were explored.
37375650	5	24	dep	UC	824:825	arg1	preventing					834:843	preventing	834:843	preventing the increase in disease activity index and the shortening of colon length	834:917	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	2	25	theme	ulcerative	423:432	arg1	UC					443:444	UC	443:444	UC	443:444	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	2	25	theme	ulcerative	423:432	arg1	colitis					434:440	ulcerative colitis	423:440	ulcerative colitis (UC)	423:445	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	5	26	theme	probiotics	1413:1422	arg1	abundance					1390:1398	the relative abundance	1377:1398	the relative abundance of potential probiotics	1377:1422	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	27	theme	colonic	942:948	arg1	inflammation					950:961	the colonic inflammation	938:961	the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus)	938:1462	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	28	dep	structure	1349:1357	arg1	such					1425:1428	such	1425:1428	such	1425:1428	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	29	from	shortening	892:901	arg1	index					878:882	disease activity index	861:882	disease activity index	861:882	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	30	theme	oxidative	1122:1130	arg1	damage					1132:1137	the oxidative damage	1118:1137	the oxidative damage	1118:1137	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	31	theme	gut	1241:1243	arg1	barrier					1245:1251	gut barrier	1241:1251	gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels)	1241:1312	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	2	32	dep	Companilactobacillus	331:350	arg1	crustorum					352:360	crustorum	352:360	crustorum	352:360	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	0	33	theme	Companilactobacillus	42:61	arg1	crustorum					63:71	Heat-Inactivated Companilactobacillus crustorum	25:71	Heat-Inactivated Companilactobacillus crustorum	25:71	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	5	34	theme	UC-related	744:753	arg1	parameters					768:777	the UC-related pathological parameters	740:777	the UC-related pathological parameters	740:777	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	1	35	theme	health	227:232	arg1	effects					234:240	promising potential health effects	207:240	promising potential health effects	207:240	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	5	36	theme	disease	861:867	arg1	index					878:882	disease activity index	861:882	disease activity index	861:882	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	0	37	theme	Heat-Inactivated	25:40	arg1	crustorum					63:71	Heat-Inactivated Companilactobacillus crustorum	25:71	Heat-Inactivated Companilactobacillus crustorum	25:71	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	5	38	theme	pathological	755:766	arg1	parameters					768:777	the UC-related pathological parameters	740:777	the UC-related pathological parameters	740:777	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	MCP-1					1095:1099	MCP-1	1095:1099	MCP-1	1095:1099	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	Cxcl1					1051:1055	Cxcl1	1051:1055	Cxcl1	1051:1055	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	TNF-α					1071:1075	TNF-α	1071:1075	TNF-α	1071:1075	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	IL-1β					1078:1082	IL-1β	1078:1082	IL-1β	1078:1082	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	cytokines					1032:1040	pro-inflammatory cytokines	1015:1040	pro-inflammatory cytokines	1015:1040	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	Cxcl5					1058:1062	Cxcl5	1058:1062	Cxcl5	1058:1062	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	Ccl7					1065:1068	Ccl7	1065:1068	Ccl7	1065:1068	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	39	theme	pro-inflammatory	1015:1030	arg1	IL-6					1085:1088	IL-6	1085:1088	IL-6	1085:1088	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	40	theme	activity	869:876	arg1	index					878:882	disease activity index	861:882	disease activity index	861:882	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	3	41	theme	UC-relieving	483:494	arg1	effect					496:501	the UC-relieving effect	479:501	the UC-relieving effect of this strain	479:516	However, it is unclear whether the UC-relieving effect of this strain is partly attributed to its bacterial composition.
37375650	5	42	theme	UC	824:825	arg1	lesions					813:819	the pathologic lesions	798:819	the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length)	798:918	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	43	theme	relative	1381:1388	arg1	abundance					1390:1398	the relative abundance	1377:1398	the relative abundance of potential probiotics	1377:1422	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	44	theme	cytokines	1032:1040	arg1	expressions					985:995	the expressions	981:995	the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1	981:1099	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	1	45	theme	Heat-inactivated	135:150	arg1	class					181:185	a typical class	171:185	a typical class of postbiotics with promising potential health effects	171:240	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	1	45	theme	Heat-inactivated	135:150	arg1	microorganisms					152:165	Heat-inactivated microorganisms	135:165	Heat-inactivated microorganisms	135:165	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	0	46	theme	crustorum	63:71	arg1	Effect					15:20	The Protective Effect	0:20	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate	0:90	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	2	47	with	supplementation	310:324	arg1	CC					369:370	CC	369:370	CC	369:370	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	2	47	with	supplementation	310:324	arg1	MN047					362:366	Companilactobacillus crustorum MN047	331:366	Companilactobacillus crustorum MN047 (CC)	331:371	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	1	48	contain	contain	251:257	arg2	components					290:299	various physiologically active components	259:299	various physiologically active components	259:299	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	1	48	contain	contain	251:257	arg1	they					246:249	they	246:249	they	246:249	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	5	49	from	increase	849:856	arg1	index					878:882	disease activity index	861:882	disease activity index	861:882	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	50	dep	inhibiting	970:979	arg1	suppressing					1146:1156	suppressing	1146:1156	suppressing the increase in myeloperoxidase and malondialdehyde	1146:1208	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	50	dep	inhibiting	970:979	arg1	e.g.					964:967	e.g.	964:967	e.g.	964:967	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	50	dep	inhibiting	970:979	arg1	mitigating					1216:1225	mitigating	1216:1225	mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels)	1216:1312	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	50	dep	inhibiting	970:979	arg1	modulating					1323:1332	modulating	1323:1332	modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus)	1323:1462	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	50	dep	inhibiting	970:979	arg1	4					1213:1213	4	1213:1213	4	1213:1213	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	51	dep	alleviating	786:796	arg1	ameliorating					925:936	ameliorating	925:936	ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus)	925:1462	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	51	dep	alleviating	786:796	arg1	1					783:783	1	783:783	1	783:783	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	51	dep	alleviating	786:796	arg1	2					922:922	2	922:922	2	922:922	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	52	theme	chemokines	1000:1009	arg1	expressions					985:995	the expressions	981:995	the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1	981:1099	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	53	theme	HICC	709:712	arg1	administration					691:704	the administration	687:704	the administration of HICC	687:712	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	54	dep	suppressing	1146:1156	arg1	e.g.					1140:1143	e.g.	1140:1143	e.g.	1140:1143	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	55	theme	claudin	1298:1304	arg1	levels					1306:1311	claudin levels	1298:1311	claudin levels	1298:1311	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	5	56	theme	potential	1403:1411	arg1	probiotics					1413:1422	potential probiotics	1403:1422	potential probiotics	1403:1422	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	2	57	theme	Dietary	302:308	arg1	supplementation					310:324	Dietary supplementation	302:324	Dietary supplementation with Companilactobacillus crustorum MN047 (CC)	302:371	Dietary supplementation with Companilactobacillus crustorum MN047 (CC) has been shown to have the potential to alleviate ulcerative colitis (UC).
37375650	3	58	theme	strain	511:516	arg1	effect					496:501	the UC-relieving effect	479:501	the UC-relieving effect of this strain	479:516	However, it is unclear whether the UC-relieving effect of this strain is partly attributed to its bacterial composition.
37375650	1	59	theme	various	259:265	arg1	components					290:299	various physiologically active components	259:299	various physiologically active components	259:299	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	5	60	dep	such	1425:1428	arg1	increasing					1366:1375	increasing	1366:1375	increasing the relative abundance of potential probiotics	1366:1422	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	0	61	from	Colitis	118:124	arg1	Mice					129:132	Mice	129:132	Mice	129:132	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	5	62	theme	colonic	1270:1276	arg1	occludin					1278:1285	colonic occludin	1270:1285	colonic occludin	1270:1285	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	1	63	theme	typical	173:179	arg1	class					181:185	a typical class	171:185	a typical class of postbiotics with promising potential health effects	171:240	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	1	63	theme	typical	173:179	arg1	microorganisms					152:165	Heat-inactivated microorganisms	135:165	Heat-inactivated microorganisms	135:165	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
37375650	5	64	dep	increasing	1366:1375	arg1	e.g.					1360:1363	e.g.	1360:1363	e.g.	1360:1363	The results showed that the administration of HICC significantly ameliorated the UC-related pathological parameters by (1) alleviating the pathologic lesions of UC (e.g., preventing the increase in disease activity index and the shortening of colon length); (2) ameliorating the colonic inflammation (e.g., inhibiting the expressions of chemokines and pro-inflammatory cytokines, such as Cxcl1, Cxcl5, Ccl7, TNF-α, IL-1β, IL-6, and MCP-1; (3) attenuating the oxidative damage (e.g., suppressing the increase in myeloperoxidase and malondialdehyde); (4) mitigating the damage of gut barrier (e.g., promoting colonic occludin, ZO-1, and claudin levels); and (5) modulating gut microbiota structure (e.g., increasing the relative abundance of potential probiotics, such as Akkermansia and Lactobacillus).
37375650	0	65	theme	Dextran	76:82	arg1	Sulfate					84:90	Dextran Sulfate	76:90	Dextran Sulfate	76:90	The Protective Effect of Heat-Inactivated Companilactobacillus crustorum on Dextran Sulfate Sodium-Induced Ulcerative Colitis in Mice.
37375650	4	66	theme	UC	640:641	arg1	mice					643:646	UC mice	640:646	UC mice	640:646	Therefore, the interventional effects of heat-inactivated CC (HICC) on UC mice were explored.
37375650	3	67	theme	bacterial	546:554	arg1	composition					556:566	its bacterial composition	542:566	its bacterial composition	542:566	However, it is unclear whether the UC-relieving effect of this strain is partly attributed to its bacterial composition.
37375650	1	68	theme	active	283:288	arg1	components					290:299	various physiologically active components	259:299	various physiologically active components	259:299	Heat-inactivated microorganisms are a typical class of postbiotics with promising potential health effects, as they contain various physiologically active components.
35438531	4	0	theme	maternal	919:926	arg1	mice					928:931	maternal mice	919:931	maternal mice	919:931	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	9	1	theme	lactation	1942:1950	arg1	period					1952:1957	the lactation period	1938:1957	the lactation period	1938:1957	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	3	2	theme	Gut	568:570	arg1	samples					583:589	Gut microbiota samples	568:589	Gut microbiota samples	568:589	Gut microbiota samples were collected from mother mice and litters.
35438531	6	3	theme	experimental	1256:1267	arg1	colitis					1269:1275	experimental colitis	1256:1275	experimental colitis	1256:1275	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	4	4	theme	16S	651:653	arg1	sequences					665:673	16S rRNA gene sequences	651:673	16S rRNA gene sequences	651:673	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	4	5	theme	different	798:806	arg1	parts					808:812	different parts	798:812	different parts of the gastrointestinal system of the fetal mice	798:861	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	3	6	theme	mother	611:616	arg1	mice					618:621	mother mice	611:621	mother mice	611:621	Gut microbiota samples were collected from mother mice and litters.
35438531	4	7	from	present	737:743	arg1	mice					763:766	prenatal fetal mice	748:766	prenatal fetal mice	748:766	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	7	8	theme	related	1530:1536	arg1	diseases					1538:1545	related diseases	1530:1545	related diseases	1530:1545	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	4	9	theme	corresponding	892:904	arg1	parts					910:914	the corresponding gut parts	888:914	the corresponding gut parts of maternal mice	888:931	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	9	10	theme	microbiota	1965:1974	arg1	development					1976:1986	GI microbiota development	1962:1986	GI microbiota development	1962:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	7	11	theme	microbiota	1515:1524	arg1	understanding					1494:1506	understanding	1494:1506	understanding of gut microbiota and related diseases	1494:1545	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	9	12	from	importance	1924:1933	arg1	development					1976:1986	GI microbiota development	1962:1986	GI microbiota development	1962:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	1	13	theme	physiological	209:221	arg1	development					223:233	physiological development	209:233	physiological development	209:233	Early-life gastrointestinal microbiota development is crucial for physiological development and immunological homeostasis.
35438531	6	14	theme	adult	1214:1218	arg1	mice					1220:1223	adult mice	1214:1223	adult mice	1214:1223	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	0	15	theme	Colitis	126:132	arg1	Severity					96:103	the Severity	92:103	the Severity of Acute DSS-Induced Colitis in Mice	92:140	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	2	16	theme	gastrointestinal	332:347	arg1	microbiota					349:358	gastrointestinal microbiota	332:358	gastrointestinal microbiota	332:358	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	4	17	from	microbiota	690:699	arg1	system					725:730	the gastrointestinal system	704:730	the gastrointestinal system	704:730	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	7	18	theme	early	1451:1455	arg1	life					1457:1460	early life	1451:1460	early life	1451:1460	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	8	19	from	development	1670:1680	arg1	life					1691:1694	early life	1685:1694	early life	1685:1694	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	9	20	theme	GI	1886:1887	arg1	tract					1889:1893	the GI tract	1882:1893	the GI tract of mice before birth	1882:1914	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	4	21	located	present	737:743	arg1	mice					763:766	prenatal fetal mice	748:766	prenatal fetal mice	748:766	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	4	21	located	present	737:743	arg2	microbiota					690:699	microbiota	690:699	microbiota in the gastrointestinal system	690:730	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	5	22	theme	different	967:975	arg1	regions					977:983	different regions	967:983	different regions	967:983	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	7	23	theme	resident	1420:1427	arg1	microbiota					1429:1438	resident microbiota	1420:1438	resident microbiota	1420:1438	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	2	24	theme	perinatal	288:296	arg1	microbiota					298:307	perinatal microbiota	288:307	perinatal microbiota	288:307	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	0	25	from	Severity	96:103	arg1	Mice					137:140	Mice	137:140	Mice	137:140	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	4	26	theme	prenatal	748:755	arg1	mice					763:766	prenatal fetal mice	748:766	prenatal fetal mice	748:766	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	8	27	theme	early-life	1711:1720	arg1	alterations					1754:1764	early-life antibiotic exposure exacerbated alterations	1711:1764	early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis	1711:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	1	28	theme	gastrointestinal	154:169	arg1	development					182:192	Early-life gastrointestinal microbiota development	143:192	Early-life gastrointestinal microbiota development	143:192	Early-life gastrointestinal microbiota development is crucial for physiological development and immunological homeostasis.
35438531	2	29	theme	colitis	547:553	arg1	model					561:565	a dextran sulfate sodium-induced colitis mouse model	514:565	a dextran sulfate sodium-induced colitis mouse model	514:565	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	8	30	dep	found	1700:1704	arg1	alterations					1754:1764	early-life antibiotic exposure exacerbated alterations	1711:1764	early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis	1711:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	0	31	from	Development	0:10	arg1	Presence					61:68	the Presence	57:68	the Presence of Antibiotics	57:83	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	4	32	theme	gastrointestinal	821:836	arg1	system					838:843	the gastrointestinal system	817:843	the gastrointestinal system of the fetal mice	817:861	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	6	33	from	mice	1280:1283	arg1	period					1316:1321	the lactation period	1302:1321	the lactation period	1302:1321	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	5	34	from	Microbiota	934:943	arg1	samples					954:960	mucus samples	948:960	mucus samples from different regions	948:983	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	5	34	from	Microbiota	934:943	arg1	regions					977:983	different regions	967:983	different regions	967:983	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	0	35	theme	Acute	108:112	arg1	Colitis					126:132	Acute DSS-Induced Colitis	108:132	Acute DSS-Induced Colitis	108:132	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	5	36	from	regions	977:983	arg1	Microbiota					934:943	Microbiota	934:943	Microbiota in mucus samples from different regions	934:983	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	5	36	from	regions	977:983	arg1	samples					954:960	mucus samples	948:960	mucus samples from different regions	948:983	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	6	37	theme	early	1130:1134	arg1	life					1136:1139	early life	1130:1139	early life	1130:1139	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	8	38	theme	whole	1610:1614	arg1	tract					1638:1642	the whole gastrointestinal (GI) tract	1606:1642	the whole gastrointestinal (GI) tract	1606:1642	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	2	39	dep	periods	384:390	arg1	periods					441:447	postweaning nutrition periods	419:447	postweaning nutrition periods	419:447	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	2	39	dep	periods	384:390	arg1	lactation					404:412	lactation	404:412	lactation	404:412	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	2	39	dep	periods	384:390	arg1	perinatal					393:401	perinatal	393:401	perinatal	393:401	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	8	40	theme	early	1685:1689	arg1	life					1691:1694	early life	1685:1694	early life	1685:1694	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	2	41	theme	nutrition	431:439	arg1	periods					441:447	postweaning nutrition periods	419:447	postweaning nutrition periods	419:447	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	2	42	theme	antibiotic-interfered	476:496	arg1	model					504:508	an antibiotic-interfered mouse model	473:508	an antibiotic-interfered mouse model	473:508	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	0	43	theme	Early-Life	15:24	arg1	Microbiota					43:52	Early-Life Gastrointestinal Microbiota	15:52	Early-Life Gastrointestinal Microbiota	15:52	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	2	44	from	microbiota	298:307	arg1	periods					384:390	different early-life periods	363:390	different early-life periods (perinatal, lactation, and postweaning nutrition periods)	363:448	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	2	45	theme	sulfate	524:530	arg1	model					561:565	a dextran sulfate sodium-induced colitis mouse model	514:565	a dextran sulfate sodium-induced colitis mouse model	514:565	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	0	46	theme	Microbiota	43:52	arg1	Development					0:10	Development	0:10	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics	0:83	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	9	47	theme	mice	1898:1901	arg1	importance					1924:1933	the importance	1920:1933	the importance of the lactation period in GI microbiota development	1920:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	9	47	theme	mice	1898:1901	arg1	tract					1889:1893	the GI tract	1882:1893	the GI tract of mice before birth	1882:1914	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	4	48	attach	present	737:743	arg1	mice					763:766	prenatal fetal mice	748:766	prenatal fetal mice	748:766	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	4	48	attach	present	737:743	arg2	microbiota					690:699	microbiota	690:699	microbiota in the gastrointestinal system	690:730	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	6	49	from	diversity	1170:1178	arg1	mice					1220:1223	adult mice	1214:1223	adult mice	1214:1223	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	6	50	theme	gastrointestinal	1183:1198	arg1	microbiota					1200:1209	gastrointestinal microbiota	1183:1209	gastrointestinal microbiota	1183:1209	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	8	51	theme	gut	1655:1657	arg1	development					1670:1680	gut microbiota development	1655:1680	gut microbiota development in early life	1655:1694	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	4	52	theme	fetal	852:856	arg1	mice					858:861	the fetal mice	848:861	the fetal mice	848:861	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	8	53	theme	antibiotic	1722:1731	arg1	alterations					1754:1764	early-life antibiotic exposure exacerbated alterations	1711:1764	early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis	1711:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	4	54	theme	rRNA	655:658	arg1	sequences					665:673	16S rRNA gene sequences	651:673	16S rRNA gene sequences	651:673	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	3	55	theme	microbiota	572:581	arg1	samples					583:589	Gut microbiota samples	568:589	Gut microbiota samples	568:589	Gut microbiota samples were collected from mother mice and litters.
35438531	8	56	theme	exacerbated	1742:1752	arg1	alterations					1754:1764	early-life antibiotic exposure exacerbated alterations	1711:1764	early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis	1711:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	4	57	theme	sequences	665:673	arg1	results					640:646	The results	636:646	The results of 16S rRNA gene sequences	636:673	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	6	58	from	composition	1154:1164	arg1	mice					1220:1223	adult mice	1214:1223	adult mice	1214:1223	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	7	59	theme	diseases	1538:1545	arg1	understanding					1494:1506	understanding	1494:1506	understanding of gut microbiota and related diseases	1494:1545	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	9	60	theme	period	1952:1957	arg1	importance					1924:1933	the importance	1920:1933	the importance of the lactation period in GI microbiota development	1920:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	9	60	theme	period	1952:1957	arg1	tract					1889:1893	the GI tract	1882:1893	the GI tract of mice before birth	1882:1914	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	6	61	from	treatment	1117:1125	arg1	life					1136:1139	early life	1130:1139	early life	1130:1139	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	9	62	attach	presence	1858:1865	arg2	bacteria					1870:1877	bacteria	1870:1877	bacteria	1870:1877	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	9	62	attach	presence	1858:1865	arg1	importance					1924:1933	the importance	1920:1933	the importance of the lactation period in GI microbiota development	1920:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	9	62	attach	presence	1858:1865	arg1	tract					1889:1893	the GI tract	1882:1893	the GI tract of mice before birth	1882:1914	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	4	63	theme	mice	928:931	arg1	parts					910:914	the corresponding gut parts	888:914	the corresponding gut parts of maternal mice	888:931	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	5	64	theme	mucus	948:952	arg1	samples					954:960	mucus samples	948:960	mucus samples from different regions	948:983	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	9	65	theme	GI	1962:1963	arg1	development					1976:1986	GI microbiota development	1962:1986	GI microbiota development	1962:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	7	66	theme	gut	1351:1353	arg1	evolution					1366:1374	gut microbiota evolution	1351:1374	gut microbiota evolution	1351:1374	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	8	67	theme	murine	1788:1793	arg1	colitis					1832:1838	murine dextran sulfate sodium (DSS)-induced colitis	1788:1838	murine dextran sulfate sodium (DSS)-induced colitis	1788:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	7	68	theme	gut	1511:1513	arg1	microbiota					1515:1524	gut microbiota	1511:1524	gut microbiota	1511:1524	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	4	69	theme	gastrointestinal	708:723	arg1	system					725:730	the gastrointestinal system	704:730	the gastrointestinal system	704:730	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	2	70	theme	different	363:371	arg1	periods					384:390	different early-life periods	363:390	different early-life periods (perinatal, lactation, and postweaning nutrition periods)	363:448	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	4	71	theme	gut	906:908	arg1	parts					910:914	the corresponding gut parts	888:914	the corresponding gut parts of maternal mice	888:931	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	2	72	theme	microbiota	349:358	arg1	development					317:327	the development	313:327	the development of gastrointestinal microbiota	313:358	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	2	72	theme	microbiota	349:358	arg1	microbiota					298:307	perinatal microbiota	288:307	perinatal microbiota	288:307	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	7	73	theme	microbiota	1355:1364	arg1	evolution					1366:1374	gut microbiota evolution	1351:1374	gut microbiota evolution	1351:1374	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	2	74	theme	current	273:279	arg1	study					281:285	the current study	269:285	the current study	269:285	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	1	75	theme	immunological	239:251	arg1	homeostasis					253:263	immunological homeostasis	239:263	immunological homeostasis	239:263	Early-life gastrointestinal microbiota development is crucial for physiological development and immunological homeostasis.
35438531	8	76	theme	exposure	1733:1740	arg1	alterations					1754:1764	early-life antibiotic exposure exacerbated alterations	1711:1764	early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis	1711:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	8	77	theme	resident	1583:1590	arg1	microbiota					1592:1601	resident microbiota	1583:1601	resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life	1583:1694	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	8	78	from	alterations	1754:1764	arg1	colitis					1832:1838	murine dextran sulfate sodium (DSS)-induced colitis	1788:1838	murine dextran sulfate sodium (DSS)-induced colitis	1788:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	8	78	from	alterations	1754:1764	arg1	microbiota					1773:1782	gut microbiota	1769:1782	gut microbiota	1769:1782	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	4	79	theme	microbiota	773:782	arg1	structures					784:793	microbiota structures	773:793	microbiota structures in different parts of the gastrointestinal system of the fetal mice	773:861	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	0	80	theme	Antibiotics	73:83	arg1	Presence					61:68	the Presence	57:68	the Presence of Antibiotics	57:83	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	1	81	theme	Early-life	143:152	arg1	development					182:192	Early-life gastrointestinal microbiota development	143:192	Early-life gastrointestinal microbiota development	143:192	Early-life gastrointestinal microbiota development is crucial for physiological development and immunological homeostasis.
35438531	2	82	from	development	317:327	arg1	periods					384:390	different early-life periods	363:390	different early-life periods (perinatal, lactation, and postweaning nutrition periods)	363:448	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	2	83	theme	mouse	555:559	arg1	model					561:565	a dextran sulfate sodium-induced colitis mouse model	514:565	a dextran sulfate sodium-induced colitis mouse model	514:565	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	1	84	theme	microbiota	171:180	arg1	development					182:192	Early-life gastrointestinal microbiota development	143:192	Early-life gastrointestinal microbiota development	143:192	Early-life gastrointestinal microbiota development is crucial for physiological development and immunological homeostasis.
35438531	2	85	theme	sodium-induced	532:545	arg1	model					561:565	a dextran sulfate sodium-induced colitis mouse model	514:565	a dextran sulfate sodium-induced colitis mouse model	514:565	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	4	86	theme	fetal	757:761	arg1	mice					763:766	prenatal fetal mice	748:766	prenatal fetal mice	748:766	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	8	87	theme	-induced	1823:1830	arg1	colitis					1832:1838	murine dextran sulfate sodium (DSS)-induced colitis	1788:1838	murine dextran sulfate sodium (DSS)-induced colitis	1788:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	6	88	from	susceptibility	1238:1251	arg1	mice					1280:1283	mice	1280:1283	mice	1280:1283	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	0	89	theme	DSS-Induced	114:124	arg1	Colitis					126:132	Acute DSS-Induced Colitis	108:132	Acute DSS-Induced Colitis	108:132	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	5	90	theme	other	1029:1033	arg1	periods					1035:1041	other periods	1029:1041	other periods	1029:1041	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	7	91	theme	enhanced	1399:1406	arg1	view					1408:1411	an enhanced view	1396:1411	an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases	1396:1545	This approach of exploring gut microbiota evolution is hoped to provide an enhanced view of how resident microbiota develop in early life, which in turn might facilitate understanding of gut microbiota and related diseases.
35438531	9	92	theme	bacteria	1870:1877	arg1	presence					1858:1865	the presence	1854:1865	the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development	1854:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	8	93	theme	gastrointestinal	1616:1631	arg1	tract					1638:1642	the whole gastrointestinal (GI) tract	1606:1642	the whole gastrointestinal (GI) tract	1606:1642	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	4	94	theme	system	838:843	arg1	parts					808:812	different parts	798:812	different parts of the gastrointestinal system of the fetal mice	798:861	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	8	95	from	microbiota	1592:1601	arg1	tract					1638:1642	the whole gastrointestinal (GI) tract	1606:1642	the whole gastrointestinal (GI) tract	1606:1642	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	5	96	theme	higher	995:1000	arg1	diversity					1002:1010	higher diversity	995:1010	higher diversity	995:1010	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	2	97	theme	mouse	498:502	arg1	model					504:508	an antibiotic-interfered mouse model	473:508	an antibiotic-interfered mouse model	473:508	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	8	98	theme	GI	1634:1635	arg1	tract					1638:1642	the whole gastrointestinal (GI) tract	1606:1642	the whole gastrointestinal (GI) tract	1606:1642	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	2	99	theme	postweaning	419:429	arg1	periods					441:447	postweaning nutrition periods	419:447	postweaning nutrition periods	419:447	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	0	100	theme	Gastrointestinal	26:41	arg1	Microbiota					43:52	Early-Life Gastrointestinal Microbiota	15:52	Early-Life Gastrointestinal Microbiota	15:52	Development of Early-Life Gastrointestinal Microbiota in the Presence of Antibiotics Alters the Severity of Acute DSS-Induced Colitis in Mice.
35438531	8	101	dep	IMPORTANCE	1548:1557	arg1	found					1700:1704	found	1700:1704	found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis	1700:1838	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	8	101	dep	IMPORTANCE	1548:1557	arg1	investigated					1570:1581	investigated	1570:1581	investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life	1570:1694	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	9	102	from	tract	1889:1893	arg1	development					1976:1986	GI microbiota development	1962:1986	GI microbiota development	1962:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	6	103	theme	lactation	1306:1314	arg1	period					1316:1321	the lactation period	1302:1321	the lactation period	1302:1321	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	6	104	theme	antibiotic	1106:1115	arg1	treatment					1117:1125	antibiotic treatment	1106:1125	antibiotic treatment in early life	1106:1139	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	2	105	theme	dextran	516:522	arg1	model					561:565	a dextran sulfate sodium-induced colitis mouse model	514:565	a dextran sulfate sodium-induced colitis mouse model	514:565	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	9	106	from	presence	1858:1865	arg1	importance					1924:1933	the importance	1920:1933	the importance of the lactation period in GI microbiota development	1920:1986	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	9	106	from	presence	1858:1865	arg1	tract					1889:1893	the GI tract	1882:1893	the GI tract of mice before birth	1882:1914	Furthermore, the presence of bacteria in the GI tract of mice before birth and the importance of the lactation period in GI microbiota development were confirmed.
35438531	5	107	theme	diet	1083:1086	arg1	change					1088:1093	diet change	1083:1093	diet change	1083:1093	Microbiota in mucus samples from different regions exhibited higher diversity at birth than at other periods and varied substantially over time with diet change.
35438531	6	108	theme	microbiota	1200:1209	arg1	composition					1154:1164	composition	1154:1164	composition	1154:1164	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	6	108	theme	microbiota	1200:1209	arg1	diversity					1170:1178	diversity	1170:1178	diversity	1170:1178	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	8	109	theme	microbiota	1659:1668	arg1	development					1670:1680	gut microbiota development	1655:1680	gut microbiota development in early life	1655:1694	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	6	110	dep	composition	1154:1164	arg1	the					1150:1152	the	1150:1152	the	1150:1152	Moreover, antibiotic treatment in early life affected the composition and diversity of gastrointestinal microbiota in adult mice and enhanced susceptibility to experimental colitis in mice, particularly in the lactation period.
35438531	4	111	from	structures	784:793	arg1	parts					808:812	different parts	798:812	different parts of the gastrointestinal system of the fetal mice	798:861	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	4	112	theme	gene	660:663	arg1	sequences					665:673	16S rRNA gene sequences	651:673	16S rRNA gene sequences	651:673	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	2	113	theme	early-life	373:382	arg1	periods					384:390	different early-life periods	363:390	different early-life periods (perinatal, lactation, and postweaning nutrition periods)	363:448	In the current study, perinatal microbiota and the development of gastrointestinal microbiota in different early-life periods (perinatal, lactation, and postweaning nutrition periods) were explored by using an antibiotic-interfered mouse model and a dextran sulfate sodium-induced colitis mouse model.
35438531	8	114	theme	gut	1769:1771	arg1	microbiota					1773:1782	gut microbiota	1769:1782	gut microbiota	1769:1782	IMPORTANCE This study investigated resident microbiota in the whole gastrointestinal (GI) tract to explore gut microbiota development in early life and found that early-life antibiotic exposure exacerbated alterations in gut microbiota and murine dextran sulfate sodium (DSS)-induced colitis.
35438531	4	115	theme	mice	858:861	arg1	system					838:843	the gastrointestinal system	817:843	the gastrointestinal system of the fetal mice	817:861	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
35438531	4	116	from	mice	763:766	arg1	present					737:743	present	737:743	present	737:743	The results of 16S rRNA gene sequences suggested that microbiota in the gastrointestinal system were present in prenatal fetal mice, and microbiota structures in different parts of the gastrointestinal system of the fetal mice were similar to those in the corresponding gut parts of maternal mice.
36088985	3	0	theme	male	601:604	arg1	mice					606:609	male mice	601:609	male mice	601:609	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	5	1	theme	NFATc4	1355:1360	arg1	signaling					1362:1370	NFATc4 signaling	1355:1370	NFATc4 signaling	1355:1370	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	2	theme	miR-149	1119:1125	arg1	expression					1094:1103	gene expression	1089:1103	gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis	1089:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	1	3	theme	gene	208:211	arg1	expression					213:222	gene expression	208:222	gene expression	208:222	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	4	4	theme	miR-7688-5p	855:865	arg1	Nfatc4					825:830	Nfatc4	825:830	Nfatc4	825:830	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	4	theme	miR-7688-5p	855:865	arg1	expression					841:850	gene expression	836:850	gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	836:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	0	5	from	role	2:5	arg1	cortex					41:46	the prefrontal cortex	26:46	the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model	26:109	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	5	6	theme	signaling	1362:1370	arg1	regulation					1341:1350	the regulation	1337:1350	the regulation of NFATc4 signaling by miR-149	1337:1381	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	4	7	with	pretreatment	915:926	arg1	10 mg/kg					947:954	10 mg/kg	947:954	10 mg/kg	947:954	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	7	with	pretreatment	915:926	arg1	-ketamine					936:944	(R)-ketamine	933:944	(R)-ketamine (10 mg/kg)	933:955	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	1	8	theme	expression	213:222	arg1	repressors					194:203	post-transcriptional repressors	173:203	post-transcriptional repressors of gene expression	173:222	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	1	8	theme	expression	213:222	arg1	RNAs					156:159	short, regulatory RNAs	138:159	short, regulatory RNAs that act as post-transcriptional repressors of gene expression	138:222	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	0	9	theme	R	77:77	arg1	-ketamine					79:87	(R)-ketamine	76:87	(R)-ketamine	76:87	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	5	10	theme	NFATc4	1290:1295	arg1	expression					1276:1285	LPS-induced expression	1264:1285	LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis	1264:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	4	11	dep	PFC.	874:877	arg1	showed					903:908	showed	903:908	showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	903:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	12	theme	R	934:934	arg1	10 mg/kg					947:954	10 mg/kg	947:954	10 mg/kg	947:954	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	12	theme	R	934:934	arg1	-ketamine					936:944	(R)-ketamine	933:944	(R)-ketamine (10 mg/kg)	933:955	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	13	from	Nfatc4	825:830	arg1	PFC.					874:877	PFC.	874:877	PFC.	874:877	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	2	14	theme	depression	457:466	arg1	model					448:452	lipopolysaccharide (LPS)-treated inflammation model	402:452	lipopolysaccharide (LPS)-treated inflammation model of depression	402:466	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	4	15	theme	gut	994:996	arg1	microbiota					998:1007	gut microbiota	994:1007	gut microbiota	994:1007	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	0	16	theme	-ketamine	79:87	arg1	actions					65:71	prophylactic actions	52:71	prophylactic actions of (R)-ketamine in inflammation model	52:109	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	5	17	theme	miR-149	1179:1185	arg1	inhibition					1165:1174	inhibition	1165:1174	inhibition of miR-149 by antagomiR-149	1165:1202	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	2	18	theme	sustained	333:341	arg1	effects					356:362	sustained prophylactic effects	333:362	sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression	333:466	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	0	19	theme	prefrontal	30:39	arg1	cortex					41:46	the prefrontal cortex	26:46	the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model	26:109	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	3	20	theme	mice	606:609	arg1	PFC					593:595	PFC	593:595	PFC	593:595	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	3	20	theme	mice	606:609	arg1	cortex					585:590	the prefrontal cortex	570:590	the prefrontal cortex (PFC) of male mice	570:609	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	5	21	theme	gut	1061:1063	arg1	microbiota					1065:1074	gut microbiota	1061:1074	gut microbiota	1061:1074	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	3	22	dep	miRNAs	508:513	arg1	miR-7688-5p					528:538	miR-7688-5p	528:538	miR-7688-5p	528:538	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	3	22	dep	miRNAs	508:513	arg1	miR-149					516:522	miR-149	516:522	miR-149	516:522	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	3	22	dep	miRNAs	508:513	arg1	miRNAs					508:513	miRNAs	508:513	miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4	508:565	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	4	23	theme	miR-149	740:746	arg1	expression					726:735	the expression	722:735	the expression of miR-149	722:746	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	23	theme	miR-149	740:746	arg1	expression					697:706	the expression	693:706	the expression of Nfatc4	693:716	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	2	24	theme	inflammation	435:446	arg1	model					448:452	lipopolysaccharide (LPS)-treated inflammation model	402:452	lipopolysaccharide (LPS)-treated inflammation model of depression	402:466	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	4	25	theme	microbiota	998:1007	arg1	composition					979:989	altered composition	971:989	altered composition of gut microbiota	971:1007	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	1	26	theme	short	138:142	arg1	repressors					194:203	post-transcriptional repressors	173:203	post-transcriptional repressors of gene expression	173:222	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	1	26	theme	short	138:142	arg1	RNAs					156:159	short, regulatory RNAs	138:159	short, regulatory RNAs that act as post-transcriptional repressors of gene expression	138:222	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	1	26	theme	short	138:142	arg1	MicroRNAs					112:120	MicroRNAs	112:120	MicroRNAs (or miRNAs)	112:132	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	5	27	theme	gene	1494:1497	arg1	expression					1499:1508	the gene expression	1490:1508	the gene expression of miRNAs	1490:1518	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	28	theme	network	1032:1038	arg1	analysis					1040:1047	A network analysis	1030:1047	A network analysis	1030:1047	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	29	theme	LPS-induced	1212:1222	arg1	behavior					1240:1247	LPS-induced depression-like behavior	1212:1247	LPS-induced depression-like behavior	1212:1247	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	2	30	theme	factor	264:269	arg1	signaling					303:311	the nuclear factor of activated T cells 4 (NFATc4) signaling	252:311	the nuclear factor of activated T cells 4 (NFATc4) signaling	252:311	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	0	31	theme	microRNA-149	10:21	arg1	role					2:5	A role	0:5	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.	0:110	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	0	32	theme	inflammation	92:103	arg1	model					105:109	inflammation model	92:109	inflammation model	92:109	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	4	33	theme	Nfatc4	711:716	arg1	expression					726:735	the expression	722:735	the expression of miR-149	722:746	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	33	theme	Nfatc4	711:716	arg1	expression					697:706	the expression	693:706	the expression of Nfatc4	693:716	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	2	34	theme	nuclear	256:262	arg1	NFATc4					295:300	NFATc4	295:300	NFATc4	295:300	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	2	34	theme	nuclear	256:262	arg1	factor					264:269	the nuclear factor	252:269	the nuclear factor of activated T cells 4 (NFATc4) signaling	252:311	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	5	35	theme	miRNAs	1513:1518	arg1	expression					1499:1508	the gene expression	1490:1508	the gene expression of miRNAs	1490:1518	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	2	36	theme	antidepressant	371:384	arg1	-ketamine					389:397	new antidepressant (R)-ketamine	367:397	new antidepressant (R)-ketamine	367:397	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	3	37	theme	LPS	635:637	arg1	administration					617:630	administration	617:630	administration of LPS (1.0 mg/kg)	617:649	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	5	38	theme	persistent	1404:1413	arg1	effects					1428:1434	persistent prophylactic effects	1404:1434	persistent prophylactic effects of (R)-ketamine	1404:1450	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	4	39	from	expression	811:820	arg1	PFC.					874:877	PFC.	874:877	PFC.	874:877	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	5	40	from	expression	1276:1285	arg1	PFC.					1304:1307	PFC.	1304:1307	PFC.	1304:1307	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	41	theme	gut	1462:1464	arg1	microbiota					1466:1475	gut microbiota	1462:1475	gut microbiota	1462:1475	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	42	theme	R	1440:1440	arg1	-ketamine					1442:1450	(R)-ketamine	1439:1450	(R)-ketamine	1439:1450	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	43	theme	prophylactic	1415:1426	arg1	effects					1428:1434	persistent prophylactic effects	1404:1434	persistent prophylactic effects of (R)-ketamine	1404:1450	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	0	44	from	actions	65:71	arg1	model					105:109	inflammation model	92:109	inflammation model	92:109	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	1	45	dep	short	138:142	arg1	regulatory					145:154	regulatory	145:154	regulatory	145:154	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	4	46	theme	altered	971:977	arg1	composition					979:989	altered composition	971:989	altered composition of gut microbiota	971:1007	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	47	dep	PFC.	755:758	arg1	was					766:768	was	766:768	was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	766:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	48	theme	Gut	879:881	arg1	analysis					894:901	Gut microbiota analysis	879:901	Gut microbiota analysis	879:901	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	49	theme	gene	806:809	arg1	expression					811:820	gene expression	806:820	gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	806:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	5	50	theme	gut-microbiota-brain	1539:1558	arg1	axis					1560:1563	gut-microbiota-brain axis	1539:1563	gut-microbiota-brain axis	1539:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	2	51	theme	prophylactic	343:354	arg1	effects					356:362	sustained prophylactic effects	333:362	sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression	333:466	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	4	52	theme	microbiota	883:892	arg1	analysis					894:901	Gut microbiota analysis	879:901	Gut microbiota analysis	879:901	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	53	theme	Nfatc4	825:830	arg1	expression					811:820	gene expression	806:820	gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	806:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	54	theme	expression	841:850	arg1	expression					811:820	gene expression	806:820	gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	806:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	2	55	theme	-ketamine	389:397	arg1	effects					356:362	sustained prophylactic effects	333:362	sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression	333:466	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	4	56	from	PFC.	874:877	arg1	expression					811:820	gene expression	806:820	gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	806:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	3	57	theme	miRNAs	508:513	arg1	role					500:503	the role	496:503	the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice	496:609	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	5	58	theme	LPS-induced	1264:1274	arg1	expression					1276:1285	LPS-induced expression	1264:1285	LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis	1264:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	2	59	theme	cells	286:290	arg1	NFATc4					295:300	NFATc4	295:300	NFATc4	295:300	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	2	59	theme	cells	286:290	arg1	factor					264:269	the nuclear factor	252:269	the nuclear factor of activated T cells 4 (NFATc4) signaling	252:311	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	4	60	theme	gene	836:839	arg1	expression					841:850	gene expression	836:850	gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	836:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	4	61	theme	negative	777:784	arg1	correlation					786:796	a negative correlation	775:796	a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice	775:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	5	62	dep	PFC.	1151:1154	arg1	blocked					1204:1210	blocked	1204:1210	blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis	1204:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	2	63	theme	T	284:284	arg1	cells					286:290	activated T cells 4	274:292	activated T cells 4	274:292	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	5	64	theme	-ketamine	1442:1450	arg1	effects					1428:1434	persistent prophylactic effects	1404:1434	persistent prophylactic effects of (R)-ketamine	1404:1450	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	65	theme	gene	1089:1092	arg1	expression					1094:1103	gene expression	1089:1103	gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis	1089:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	66	from	expression	1094:1103	arg1	PFC.					1151:1154	PFC.	1151:1154	PFC.	1151:1154	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	4	67	theme	positive	664:671	arg1	correlation					673:683	a positive correlation	662:683	a positive correlation between the expression of Nfatc4 and the expression of miR-149	662:746	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	2	68	from	effects	356:362	arg1	model					448:452	lipopolysaccharide (LPS)-treated inflammation model	402:452	lipopolysaccharide (LPS)-treated inflammation model of depression	402:466	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	2	69	theme	activated	274:282	arg1	cells					286:290	activated T cells 4	274:292	activated T cells 4	274:292	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	5	70	dep	PFC.	1304:1307	arg1	suggest					1324:1330	suggest	1324:1330	suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis	1324:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	1	71	theme	post-transcriptional	173:192	arg1	repressors					194:203	post-transcriptional repressors	173:203	post-transcriptional repressors of gene expression	173:222	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	1	71	theme	post-transcriptional	173:192	arg1	RNAs					156:159	short, regulatory RNAs	138:159	short, regulatory RNAs that act as post-transcriptional repressors of gene expression	138:222	MicroRNAs (or miRNAs) are short, regulatory RNAs that act as post-transcriptional repressors of gene expression.
36088985	0	72	theme	prophylactic	52:63	arg1	actions					65:71	prophylactic actions	52:71	prophylactic actions of (R)-ketamine in inflammation model	52:109	A role of microRNA-149 in the prefrontal cortex for prophylactic actions of (R)-ketamine in inflammation model.
36088985	3	73	theme	prefrontal	574:583	arg1	PFC					593:595	PFC	593:595	PFC	593:595	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	3	73	theme	prefrontal	574:583	arg1	cortex					585:590	the prefrontal cortex	570:590	the prefrontal cortex (PFC) of male mice	570:609	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	4	74	theme	LPS-treated	1012:1022	arg1	mice					1024:1027	LPS-treated mice	1012:1027	LPS-treated mice	1012:1027	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36088985	3	75	from	role	500:503	arg1	PFC					593:595	PFC	593:595	PFC	593:595	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	3	75	from	role	500:503	arg1	cortex					585:590	the prefrontal cortex	570:590	the prefrontal cortex (PFC) of male mice	570:609	In this study, we examined the role of miRNAs (miR-149 and miR-7688-5p) which can regulate NFATc4 in the prefrontal cortex (PFC) of male mice after administration of LPS (1.0 mg/kg).
36088985	2	76	theme	-treated	426:433	arg1	model					448:452	lipopolysaccharide (LPS)-treated inflammation model	402:452	lipopolysaccharide (LPS)-treated inflammation model of depression	402:466	Recently, we reported that the nuclear factor of activated T cells 4 (NFATc4) signaling might contribute to sustained prophylactic effects of new antidepressant (R)-ketamine in lipopolysaccharide (LPS)-treated inflammation model of depression.
36088985	5	77	theme	Nfatc4	1108:1113	arg1	expression					1094:1103	gene expression	1089:1103	gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis	1089:1563	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	5	78	theme	depression-like	1224:1238	arg1	behavior					1240:1247	LPS-induced depression-like behavior	1212:1247	LPS-induced depression-like behavior	1212:1247	A network analysis showed that gut microbiota may regulate gene expression of Nfatc4 and miR-149 (or miR-7688-5p) in the PFC. Finally, inhibition of miR-149 by antagomiR-149 blocked LPS-induced depression-like behavior by attenuating LPS-induced expression of NFATc4 in the PFC. These findings suggest that the regulation of NFATc4 signaling by miR-149 might play a role in persistent prophylactic effects of (R)-ketamine, and that gut microbiota may regulate the gene expression of miRNAs in the PFC through gut-microbiota-brain axis.
36088985	4	79	from	expression	841:850	arg1	PFC.					874:877	PFC.	874:877	PFC.	874:877	There was a positive correlation between the expression of Nfatc4 and the expression of miR-149 in the PFC. There was also a negative correlation between gene expression of Nfatc4 and gene expression of miR-7688-5p in the PFC. Gut microbiota analysis showed that pretreatment with (R)-ketamine (10 mg/kg) could restore altered composition of gut microbiota in LPS-treated mice.
36235177	14	0	theme	pathogenic	2239:2248	arg1	bacteria					2250:2257	pathogenic bacteria	2239:2257	pathogenic bacteria	2239:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	4	1	theme	reducing	552:559	arg1	FRAP					580:583	FRAP	580:583	FRAP	580:583	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	1	theme	reducing	552:559	arg1	power					573:577	Ferric reducing antioxidant power	545:577	Ferric reducing antioxidant power (FRAP)	545:584	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	2	2	theme	chemical	238:245	arg1	composition					247:257	The chemical composition	234:257	The chemical composition	234:257	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	14	3	theme	biological	2110:2119	arg1	capabilities					2121:2132	significant biological capabilities	2098:2132	significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria	2098:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	1	4	theme	Noë	162:164	arg1	Jury					167:170	Ballota deserti (Noë) Jury	145:170	Ballota deserti (Noë) Jury (B. deserti)	145:183	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	1	4	theme	Noë	162:164	arg1	plant					200:204	a medicinal plant	188:204	a medicinal plant used in Ayurvedic medicine	188:231	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	5	5	dep	diffusion	723:731	arg1	agar					733:736	agar	733:736	agar	733:736	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	8	6	theme	antioxidant	1121:1131	arg1	potency					1133:1139	good antioxidant potency	1116:1139	good antioxidant potency	1116:1139	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	12	7	theme	23.74	1776:1780	arg1	µg/mL					1789:1793	23.74 ± 1.54 µg/mL	1776:1793	23.74 ± 1.54 µg/mL	1776:1793	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	11	8	theme	fungal	1595:1600	arg1	strains					1602:1608	fungal strains	1595:1608	fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm	1595:1675	Similarly, impressive antifungal activity was observed against fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm.
36235177	9	9	theme	Gram-negative	1260:1272	arg1	bacteria					1292:1299	Both Gram-negative and Gram-positive bacteria	1255:1299	Both Gram-negative and Gram-positive bacteria	1255:1299	Both Gram-negative and Gram-positive bacteria were inhibited by EOBD with inhibition zones ranging from 17.481.75 mm to 28.471.44 mm.
36235177	6	10	dep	in	893:894	arg1	silico					896:901	silico	896:901	silico	896:901	An in silico approach was used to evaluate the inhibitory potential of EOBD against NADPH oxidase.
36235177	8	11	theme	calculated	1147:1156	arg1	values					1158:1163	calculated values	1147:1163	calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC)	1147:1252	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	13	12	theme	NADPH	1913:1917	arg1	oxidase					1919:1925	NADPH oxidase	1913:1925	NADPH oxidase	1913:1925	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	9	13	theme	Gram-positive	1278:1290	arg1	bacteria					1292:1299	Both Gram-negative and Gram-positive bacteria	1255:1299	Both Gram-negative and Gram-positive bacteria	1255:1299	Both Gram-negative and Gram-positive bacteria were inhibited by EOBD with inhibition zones ranging from 17.481.75 mm to 28.471.44 mm.
36235177	12	14	theme	15.32	1760:1764	arg1	±					1766:1766	15.32 ± 1.47	1760:1771	15.32 ± 1.47	1760:1771	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	11	15	theme	zone	1626:1629	arg1	widths					1631:1636	inhibition zone widths	1615:1636	inhibition zone widths ranging from 16.761.83 to 36.791.35 mm	1615:1675	Similarly, impressive antifungal activity was observed against fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm.
36235177	7	16	theme	EOBD	1002:1005	arg1	%					1015:1015	0.41%	1011:1015	0.41%	1011:1015	The yield of EOBD was 0.41%, and was primarily composed of linalool (37.82%), cineole (12.04%), and borneol (11.07%).
36235177	7	16	theme	EOBD	1002:1005	arg1	yield					993:997	The yield	989:997	The yield of EOBD	989:1005	The yield of EOBD was 0.41%, and was primarily composed of linalool (37.82%), cineole (12.04%), and borneol (11.07%).
36235177	6	17	theme	in	893:894	arg1	approach					903:910	An in silico approach	890:910	An in silico approach	890:910	An in silico approach was used to evaluate the inhibitory potential of EOBD against NADPH oxidase.
36235177	8	18	theme	±	1174:1174	arg1	µg/mL					1181:1185	19.82 ± 1.14 µg/mL	1168:1185	19.82 ± 1.14 µg/mL (DPPH)	1168:1192	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	18	theme	±	1174:1174	arg1	mg					1245:1246	996.84 ± 20.18 µg EAA/ mg	1222:1246	996.84 ± 20.18 µg EAA/ mg (TAC)	1222:1252	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	18	theme	±	1174:1174	arg1	µg/mL					1208:1212	64.78 ± 5.21 µg/mL	1195:1212	64.78 ± 5.21 µg/mL (FRAP)	1195:1219	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	18	theme	±	1174:1174	arg1	DPPH					1188:1191	DPPH	1188:1191	DPPH	1188:1191	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	11	19	theme	impressive	1543:1552	arg1	activity					1565:1572	impressive antifungal activity	1543:1572	impressive antifungal activity	1543:1572	Similarly, impressive antifungal activity was observed against fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm.
36235177	2	20	from	composition	247:257	arg1	EOBD					349:352	EOBD	349:352	EOBD	349:352	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	20	from	composition	247:257	arg1	deserti					340:346	B. deserti	337:346	B. deserti (EOBD) against drug-resistant microorganisms	337:391	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	8	21	theme	µg/mL	1181:1185	arg1	values					1158:1163	calculated values	1147:1163	calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC)	1147:1252	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	14	22	theme	potential	2054:2062	arg1	source					2064:2069	a potential source	2052:2069	a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria	2052:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	14	22	theme	potential	2054:2062	arg1	EOBD					2032:2035	the EOBD	2028:2035	the EOBD	2028:2035	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	13	23	theme	glide	1933:1937	arg1	scores					1939:1944	glide scores	1933:1944	glide scores of -5.403, -5.344, and -4.973 Kcal/mol	1933:1983	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	2	24	from	antioxidant	260:270	arg1	EOBD					349:352	EOBD	349:352	EOBD	349:352	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	24	from	antioxidant	260:270	arg1	deserti					340:346	B. deserti	337:346	B. deserti (EOBD) against drug-resistant microorganisms	337:391	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	0	25	theme	Chemically-Analyzed	69:87	arg1	Oils					99:102	Chemically-Analyzed Essential Oils	69:102	Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury	69:142	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	1	26	theme	deserti	153:159	arg1	Jury					167:170	Ballota deserti (Noë) Jury	145:170	Ballota deserti (Noë) Jury (B. deserti)	145:183	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	1	26	theme	deserti	153:159	arg1	plant					200:204	a medicinal plant	188:204	a medicinal plant used in Ayurvedic medicine	188:231	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	2	27	from	antibacterial	273:285	arg1	EOBD					349:352	EOBD	349:352	EOBD	349:352	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	27	from	antibacterial	273:285	arg1	deserti					340:346	B. deserti	337:346	B. deserti (EOBD) against drug-resistant microorganisms	337:391	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	9	28	with	EOBD	1319:1322	arg1	zones					1340:1344	inhibition zones	1329:1344	inhibition zones ranging from 17.481.75 mm to 28.471.44 mm	1329:1386	Both Gram-negative and Gram-positive bacteria were inhibited by EOBD with inhibition zones ranging from 17.481.75 mm to 28.471.44 mm.
36235177	0	29	theme	Oils	99:102	arg1	Antimicrobial					13:25	Antimicrobial	13:25	Antimicrobial	13:25	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	0	29	theme	Oils	99:102	arg1	Antioxidant					0:10	Antioxidant	0:10	Antioxidant	0:10	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	0	29	theme	Oils	99:102	arg1	Inhibition					55:64	In Silico NADPH Oxidase Inhibition	31:64	In Silico NADPH Oxidase Inhibition	31:64	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	6	30	theme	inhibitory	937:946	arg1	potential					948:956	the inhibitory potential	933:956	the inhibitory potential of EOBD against NADPH oxidase	933:986	An in silico approach was used to evaluate the inhibitory potential of EOBD against NADPH oxidase.
36235177	8	31	theme	±	1201:1201	arg1	µg/mL					1208:1212	64.78 ± 5.21 µg/mL	1195:1212	64.78 ± 5.21 µg/mL (FRAP)	1195:1219	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	31	theme	±	1201:1201	arg1	µg/mL					1181:1185	19.82 ± 1.14 µg/mL	1168:1185	19.82 ± 1.14 µg/mL (DPPH)	1168:1192	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	31	theme	±	1201:1201	arg1	FRAP					1215:1218	FRAP	1215:1218	FRAP	1215:1218	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	12	32	theme	assay	1694:1698	arg1	Results					1678:1684	Results	1678:1684	Results of MICs assay against fungi	1678:1712	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	4	33	theme	antioxidant	686:696	arg1	effect					698:703	the antioxidant effect	682:703	the antioxidant effect of EOBD	682:711	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	9	34	theme	28.471.44	1375:1383	arg1	mm					1369:1370	mm	1369:1370	mm	1369:1370	Both Gram-negative and Gram-positive bacteria were inhibited by EOBD with inhibition zones ranging from 17.481.75 mm to 28.471.44 mm.
36235177	12	35	contain	had	1731:1733	arg1	EOBD					1726:1729	EOBD	1726:1729	EOBD	1726:1729	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	12	35	contain	had	1731:1733	arg2	values					1740:1745	MICs values	1735:1745	MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL	1735:1793	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	0	36	theme	In	31:32	arg1	Oxidase					47:53	In Silico NADPH Oxidase	31:53	In Silico NADPH Oxidase Inhibition	31:64	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	2	37	from	deserti	340:346	arg1	antioxidant					260:270	antioxidant	260:270	antioxidant	260:270	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	37	from	deserti	340:346	arg1	properties					303:312	antifungal properties	292:312	antifungal properties	292:312	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	37	from	deserti	340:346	arg1	composition					247:257	The chemical composition	234:257	The chemical composition	234:257	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	37	from	deserti	340:346	arg1	oils					327:330	essential oils	317:330	essential oils from B. deserti (EOBD) against drug-resistant microorganisms	317:391	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	37	from	deserti	340:346	arg1	antibacterial					273:285	antibacterial	273:285	antibacterial	273:285	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	3	38	theme	extract	451:457	arg1	EOBD					459:462	extract EOBD	451:462	extract EOBD	451:462	Hydrodistillation was used to extract EOBD, and gas chromatography-mass spectrometry was used to identify its constituents.
36235177	0	39	theme	NADPH	41:45	arg1	Oxidase					47:53	In Silico NADPH Oxidase	31:53	In Silico NADPH Oxidase Inhibition	31:64	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	0	40	dep	In	31:32	arg1	Silico					34:39	Silico	34:39	Silico	34:39	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	8	41	theme	µg	1237:1238	arg1	µg/mL					1181:1185	19.82 ± 1.14 µg/mL	1168:1185	19.82 ± 1.14 µg/mL (DPPH)	1168:1192	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	41	theme	µg	1237:1238	arg1	TAC					1249:1251	TAC	1249:1251	TAC	1249:1251	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	41	theme	µg	1237:1238	arg1	mg					1245:1246	996.84 ± 20.18 µg EAA/ mg	1222:1246	996.84 ± 20.18 µg EAA/ mg (TAC)	1222:1252	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	3	42	used	used	443:446	arg2	Hydrodistillation					421:437	Hydrodistillation	421:437	Hydrodistillation	421:437	Hydrodistillation was used to extract EOBD, and gas chromatography-mass spectrometry was used to identify its constituents.
36235177	10	43	theme	inhibitory	1494:1503	arg1	concentration					1505:1517	the minimum inhibitory concentration	1482:1517	the minimum inhibitory concentration (MIC) assay	1482:1529	EOBD exhibited MICs ranging from 10.78 g/mL to 22.48 g/mL when tested against bacteria using the minimum inhibitory concentration (MIC) assay.
36235177	10	43	theme	inhibitory	1494:1503	arg1	MIC					1520:1522	MIC	1520:1522	MIC	1520:1522	EOBD exhibited MICs ranging from 10.78 g/mL to 22.48 g/mL when tested against bacteria using the minimum inhibitory concentration (MIC) assay.
36235177	14	44	theme	effective	2198:2206	arg1	weapon					2208:2213	an effective weapon	2195:2213	an effective weapon against a wide range of pathogenic bacteria	2195:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	14	45	theme	natural	2162:2168	arg1	antioxidants					2170:2181	natural antioxidants	2162:2181	natural antioxidants	2162:2181	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	3	46	theme	gas	469:471	arg1	spectrometry					493:504	gas chromatography-mass spectrometry	469:504	gas chromatography-mass spectrometry	469:504	Hydrodistillation was used to extract EOBD, and gas chromatography-mass spectrometry was used to identify its constituents.
36235177	5	47	theme	antibacterial	801:813	arg1	properties					815:824	the antibacterial properties	797:824	the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms	797:887	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	1	48	theme	medicinal	190:198	arg1	plant					200:204	a medicinal plant	188:204	a medicinal plant used in Ayurvedic medicine	188:231	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	1	48	theme	medicinal	190:198	arg1	Jury					167:170	Ballota deserti (Noë) Jury	145:170	Ballota deserti (Noë) Jury (B. deserti)	145:183	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	14	49	theme	bacteria	2250:2257	arg1	range					2230:2234	a wide range	2223:2234	a wide range of pathogenic bacteria	2223:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	2	50	theme	essential	317:325	arg1	oils					327:330	essential oils	317:330	essential oils from B. deserti (EOBD) against drug-resistant microorganisms	317:391	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	13	51	theme	butanoic	1847:1854	arg1	acid					1856:1859	butanoic acid	1847:1859	butanoic acid	1847:1859	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	4	52	theme	Ferric	545:550	arg1	FRAP					580:583	FRAP	580:583	FRAP	580:583	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	52	theme	Ferric	545:550	arg1	power					573:577	Ferric reducing antioxidant power	545:577	Ferric reducing antioxidant power (FRAP)	545:584	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	1	53	theme	Ayurvedic	214:222	arg1	medicine					224:231	Ayurvedic medicine	214:231	Ayurvedic medicine	214:231	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	12	54	theme	±	1782:1782	arg1	µg/mL					1789:1793	23.74 ± 1.54 µg/mL	1776:1793	23.74 ± 1.54 µg/mL	1776:1793	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	14	55	theme	wide	2225:2228	arg1	range					2230:2234	a wide range	2223:2234	a wide range of pathogenic bacteria	2223:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	2	56	from	properties	303:312	arg1	EOBD					349:352	EOBD	349:352	EOBD	349:352	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	56	from	properties	303:312	arg1	deserti					340:346	B. deserti	337:346	B. deserti (EOBD) against drug-resistant microorganisms	337:391	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	9	57	theme	inhibition	1329:1338	arg1	zones					1340:1344	inhibition zones	1329:1344	inhibition zones ranging from 17.481.75 mm to 28.471.44 mm	1329:1386	Both Gram-negative and Gram-positive bacteria were inhibited by EOBD with inhibition zones ranging from 17.481.75 mm to 28.471.44 mm.
36235177	4	58	theme	antioxidant	561:571	arg1	FRAP					580:583	FRAP	580:583	FRAP	580:583	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	58	theme	antioxidant	561:571	arg1	power					573:577	Ferric reducing antioxidant power	545:577	Ferric reducing antioxidant power (FRAP)	545:584	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	59	theme	antioxidant	635:645	arg1	TAC					657:659	TAC	657:659	TAC	657:659	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	59	theme	antioxidant	635:645	arg1	capacity					647:654	total antioxidant capacity	629:654	total antioxidant capacity (TAC)	629:660	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	5	60	theme	disc	718:721	arg1	diffusion					723:731	The disc diffusion agar and the microdilution tests	714:764	diffusion	723:731	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	14	61	theme	significant	2098:2108	arg1	capabilities					2121:2132	significant biological capabilities	2098:2132	significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria	2098:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	5	62	theme	properties	815:824	arg1	assessment					783:792	the assessment	779:792	the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms	779:887	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	8	63	contain	had	1112:1114	arg1	EOBD					1107:1110	EOBD	1107:1110	EOBD	1107:1110	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	63	contain	had	1112:1114	arg2	potency					1133:1139	good antioxidant potency	1116:1139	good antioxidant potency	1116:1139	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	3	64	used	used	510:513	arg2	spectrometry					493:504	gas chromatography-mass spectrometry	469:504	gas chromatography-mass spectrometry	469:504	Hydrodistillation was used to extract EOBD, and gas chromatography-mass spectrometry was used to identify its constituents.
36235177	11	65	theme	inhibition	1615:1624	arg1	widths					1631:1636	inhibition zone widths	1615:1636	inhibition zone widths ranging from 16.761.83 to 36.791.35 mm	1615:1675	Similarly, impressive antifungal activity was observed against fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm.
36235177	14	66	theme	compounds	2083:2091	arg1	source					2064:2069	a potential source	2052:2069	a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria	2052:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	14	66	theme	compounds	2083:2091	arg1	EOBD					2032:2035	the EOBD	2028:2035	the EOBD	2028:2035	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	5	67	theme	resistant	853:861	arg1	microorganisms					874:887	clinically resistant pathogenic microorganisms	842:887	clinically resistant pathogenic microorganisms	842:887	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	10	68	theme	concentration	1505:1517	arg1	assay					1525:1529	the minimum inhibitory concentration (MIC) assay	1482:1529	the minimum inhibitory concentration (MIC) assay	1482:1529	EOBD exhibited MICs ranging from 10.78 g/mL to 22.48 g/mL when tested against bacteria using the minimum inhibitory concentration (MIC) assay.
36235177	4	69	theme	total	629:633	arg1	TAC					657:659	TAC	657:659	TAC	657:659	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	69	theme	total	629:633	arg1	capacity					647:654	total antioxidant capacity	629:654	total antioxidant capacity (TAC)	629:660	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	6	70	used	used	916:919	arg2	approach					903:910	An in silico approach	890:910	An in silico approach	890:910	An in silico approach was used to evaluate the inhibitory potential of EOBD against NADPH oxidase.
36235177	5	71	theme	pathogenic	863:872	arg1	microorganisms					874:887	clinically resistant pathogenic microorganisms	842:887	clinically resistant pathogenic microorganisms	842:887	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	9	72	dep	mm	1369:1370	arg1	to					1372:1373	to	1372:1373	to	1372:1373	Both Gram-negative and Gram-positive bacteria were inhibited by EOBD with inhibition zones ranging from 17.481.75 mm to 28.471.44 mm.
36235177	14	73	theme	chemical	2074:2081	arg1	compounds					2083:2091	chemical compounds	2074:2091	chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria	2074:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	11	74	theme	antifungal	1554:1563	arg1	activity					1565:1572	impressive antifungal activity	1543:1572	impressive antifungal activity	1543:1572	Similarly, impressive antifungal activity was observed against fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm.
36235177	13	75	theme	strong	1889:1894	arg1	activity					1896:1903	strong activity	1889:1903	strong activity against NADPH oxidase	1889:1925	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	5	76	theme	EOBD	829:832	arg1	properties					815:824	the antibacterial properties	797:824	the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms	797:887	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	6	77	theme	NADPH	974:978	arg1	oxidase					980:986	NADPH oxidase	974:986	NADPH oxidase	974:986	An in silico approach was used to evaluate the inhibitory potential of EOBD against NADPH oxidase.
36235177	0	78	theme	Essential	89:97	arg1	Oils					99:102	Chemically-Analyzed Essential Oils	69:102	Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury	69:142	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	8	79	theme	good	1116:1119	arg1	potency					1133:1139	good antioxidant potency	1116:1139	good antioxidant potency	1116:1139	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	1	80	theme	Ballota	145:151	arg1	Jury					167:170	Ballota deserti (Noë) Jury	145:170	Ballota deserti (Noë) Jury (B. deserti)	145:183	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	1	80	theme	Ballota	145:151	arg1	plant					200:204	a medicinal plant	188:204	a medicinal plant used in Ayurvedic medicine	188:231	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	6	81	theme	EOBD	961:964	arg1	potential					948:956	the inhibitory potential	933:956	the inhibitory potential of EOBD against NADPH oxidase	933:986	An in silico approach was used to evaluate the inhibitory potential of EOBD against NADPH oxidase.
36235177	12	82	theme	MICs	1735:1738	arg1	values					1740:1745	MICs values	1735:1745	MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL	1735:1793	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	4	83	theme	EOBD	708:711	arg1	effect					698:703	the antioxidant effect	682:703	the antioxidant effect of EOBD	682:711	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	84	used	used	667:670	arg2	capacity					647:654	total antioxidant capacity	629:654	total antioxidant capacity (TAC)	629:660	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	84	used	used	667:670	arg2	TAC					657:659	TAC	657:659	TAC	657:659	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	84	used	used	667:670	arg2	power					573:577	Ferric reducing antioxidant power	545:577	Ferric reducing antioxidant power (FRAP)	545:584	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	84	used	used	667:670	arg2	1,1-diphenyl-2-picrylhydrazyl					587:615	1,1-diphenyl-2-picrylhydrazyl	587:615	1,1-diphenyl-2-picrylhydrazyl (DPPH)	587:622	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	84	used	used	667:670	arg2	FRAP					580:583	FRAP	580:583	FRAP	580:583	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	4	84	used	used	667:670	arg2	DPPH					618:621	DPPH	618:621	DPPH	618:621	Ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH), and total antioxidant capacity (TAC) were used to assess the antioxidant effect of EOBD.
36235177	11	85	dep	36.791.35	1664:1672	arg1	to					1661:1662	to	1661:1662	to	1661:1662	Similarly, impressive antifungal activity was observed against fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm.
36235177	8	86	theme	64.78	1195:1199	arg1	µg/mL					1208:1212	64.78 ± 5.21 µg/mL	1195:1212	64.78 ± 5.21 µg/mL (FRAP)	1195:1219	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	86	theme	64.78	1195:1199	arg1	µg/mL					1181:1185	19.82 ± 1.14 µg/mL	1168:1185	19.82 ± 1.14 µg/mL (DPPH)	1168:1192	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	86	theme	64.78	1195:1199	arg1	FRAP					1215:1218	FRAP	1215:1218	FRAP	1215:1218	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	5	87	theme	microdilution	746:758	arg1	tests					760:764	the microdilution tests	742:764	the microdilution tests	742:764	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	11	88	with	strains	1602:1608	arg1	widths					1631:1636	inhibition zone widths	1615:1636	inhibition zone widths ranging from 16.761.83 to 36.791.35 mm	1615:1675	Similarly, impressive antifungal activity was observed against fungal strains with inhibition zone widths ranging from 16.761.83 to 36.791.35 mm.
36235177	0	89	dep	deserti	125:131	arg1	Jury					139:142	Jury	139:142	Ballota deserti (Noë) Jury	117:142	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	14	90	theme	findings	2018:2025	arg1	light					2003:2007	light	2003:2007	light of these findings	2003:2025	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	13	91	contain	possessed	1879:1887	arg1	o-cymene					1833:1840	o-cymene	1833:1840	o-cymene	1833:1840	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	13	91	contain	possessed	1879:1887	arg1	thujone					1824:1830	thujone	1824:1830	thujone	1824:1830	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	13	91	contain	possessed	1879:1887	arg1	acid					1856:1859	butanoic acid	1847:1859	butanoic acid	1847:1859	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	13	91	contain	possessed	1879:1887	arg2	activity					1896:1903	strong activity	1889:1903	strong activity against NADPH oxidase	1889:1925	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	9	92	theme	17.481.75	1359:1367	arg1	mm					1369:1370	mm	1369:1370	mm	1369:1370	Both Gram-negative and Gram-positive bacteria were inhibited by EOBD with inhibition zones ranging from 17.481.75 mm to 28.471.44 mm.
36235177	2	93	theme	drug-resistant	363:376	arg1	microorganisms					378:391	drug-resistant microorganisms	363:391	drug-resistant microorganisms	363:391	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	12	94	theme	MICs	1689:1692	arg1	assay					1694:1698	MICs assay	1689:1698	MICs assay against fungi	1689:1712	Results of MICs assay against fungi showed that EOBD had MICs values ranging from 15.32 ± 1.47 to 23.74 ± 1.54 µg/mL.
36235177	1	95	dep	Jury	167:170	arg1	deserti					176:182	B. deserti	173:182	B. deserti	173:182	Ballota deserti (Noë) Jury (B. deserti) is a medicinal plant used in Ayurvedic medicine.
36235177	13	96	theme	Docking	1796:1802	arg1	results					1804:1810	Docking results	1796:1810	Docking results	1796:1810	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	0	97	theme	Oxidase	47:53	arg1	Inhibition					55:64	In Silico NADPH Oxidase Inhibition	31:64	In Silico NADPH Oxidase Inhibition	31:64	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	5	98	used	used	771:774	arg2	diffusion					723:731	The disc diffusion agar and the microdilution tests	714:764	diffusion	723:731	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	5	98	used	used	771:774	arg2	tests					760:764	the microdilution tests	742:764	the microdilution tests	742:764	The disc diffusion agar and the microdilution tests were used in the assessment of the antibacterial properties of EOBD against clinically resistant pathogenic microorganisms.
36235177	2	99	theme	oils	327:330	arg1	antioxidant					260:270	antioxidant	260:270	antioxidant	260:270	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	99	theme	oils	327:330	arg1	composition					247:257	The chemical composition	234:257	The chemical composition	234:257	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	99	theme	oils	327:330	arg1	properties					303:312	antifungal properties	292:312	antifungal properties	292:312	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	2	99	theme	oils	327:330	arg1	antibacterial					273:285	antibacterial	273:285	antibacterial	273:285	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	14	100	with	compounds	2083:2091	arg1	capabilities					2121:2132	significant biological capabilities	2098:2132	significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria	2098:2257	In light of these findings, the EOBD may be seen as a potential source of chemical compounds with significant biological capabilities that can be advantageous as natural antioxidants and develop an effective weapon against a wide range of pathogenic bacteria.
36235177	2	101	theme	antifungal	292:301	arg1	properties					303:312	antifungal properties	292:312	antifungal properties	292:312	The chemical composition, antioxidant, antibacterial, and antifungal properties of essential oils from B. deserti (EOBD) against drug-resistant microorganisms were examined in this work.
36235177	13	102	theme	Kcal/mol	1976:1983	arg1	scores					1939:1944	glide scores	1933:1944	glide scores of -5.403, -5.344, and -4.973 Kcal/mol	1933:1983	Docking results showed that thujone, o-cymene, and butanoic acid contained in EOBD possessed strong activity against NADPH oxidase, with glide scores of -5.403, -5.344, and -4.973 Kcal/mol, respectively.
36235177	10	103	theme	minimum	1486:1492	arg1	concentration					1505:1517	the minimum inhibitory concentration	1482:1517	the minimum inhibitory concentration (MIC) assay	1482:1529	EOBD exhibited MICs ranging from 10.78 g/mL to 22.48 g/mL when tested against bacteria using the minimum inhibitory concentration (MIC) assay.
36235177	10	103	theme	minimum	1486:1492	arg1	MIC					1520:1522	MIC	1520:1522	MIC	1520:1522	EOBD exhibited MICs ranging from 10.78 g/mL to 22.48 g/mL when tested against bacteria using the minimum inhibitory concentration (MIC) assay.
36235177	0	104	attach	Derived	104:110	arg1	Noë					134:136	Noë	134:136	Noë	134:136	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	0	104	attach	Derived	104:110	arg1	deserti					125:131	deserti	125:131	deserti	125:131	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	0	104	attach	Derived	104:110	arg2	Oils					99:102	Chemically-Analyzed Essential Oils	69:102	Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury	69:142	Antioxidant, Antimicrobial and In Silico NADPH Oxidase Inhibition of Chemically-Analyzed Essential Oils Derived from Ballota deserti (Noë) Jury.
36235177	8	105	theme	EAA/	1240:1243	arg1	µg/mL					1181:1185	19.82 ± 1.14 µg/mL	1168:1185	19.82 ± 1.14 µg/mL (DPPH)	1168:1192	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	105	theme	EAA/	1240:1243	arg1	TAC					1249:1251	TAC	1249:1251	TAC	1249:1251	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	8	105	theme	EAA/	1240:1243	arg1	mg					1245:1246	996.84 ± 20.18 µg EAA/ mg	1222:1246	996.84 ± 20.18 µg EAA/ mg (TAC)	1222:1252	EOBD had good antioxidant potency, with calculated values of 19.82 ± 1.14 µg/mL (DPPH), 64.78 ± 5.21 µg/mL (FRAP), 996.84 ± 20.18 µg EAA/ mg (TAC).
36235177	3	106	theme	chromatography-mass	473:491	arg1	spectrometry					493:504	gas chromatography-mass spectrometry	469:504	gas chromatography-mass spectrometry	469:504	Hydrodistillation was used to extract EOBD, and gas chromatography-mass spectrometry was used to identify its constituents.
36152417	8	0	theme	microbial	1031:1039	arg1	compositions					1041:1052	the microbial compositions	1027:1052	the microbial compositions inhabited on the intestinal mucosa	1027:1087	GQD modulated the microbial compositions inhabited on the intestinal mucosa, especially an increase of the Lactobacillus.
36152417	8	0	theme	microbial	1031:1039	arg1	increase					1104:1111	an increase	1101:1111	an increase of the Lactobacillus	1101:1132	GQD modulated the microbial compositions inhabited on the intestinal mucosa, especially an increase of the Lactobacillus.
36152417	1	1	theme	bacterial	171:179	arg1	problem					209:215	a severe global problem	193:215	a severe global problem with a particularly high incidence rate in children	193:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	1	1	theme	bacterial	171:179	arg1	diarrhea					181:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	7	2	theme	barrier	1004:1010	arg1	restoration					978:988	the restoration	974:988	the restoration of intestinal barrier	974:1010	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	5	3	theme	bacterial	635:643	arg1	diarrhea					645:652	bacterial diarrhea	635:652	bacterial diarrhea	635:652	A Tibetan piglet model with bacterial diarrhea was established through orally administered Escherichia coli, and diarrheal piglets were treated with GQD for three days.
36152417	0	4	theme	Lactobacillus	99:111	arg1	growth					113:118	Lactobacillus growth	99:118	Lactobacillus growth	99:118	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	7	5	theme	occludin	925:932	arg1	levels					934:939	increased SIgA, ZO-1, and occludin levels	899:939	increased SIgA, ZO-1, and occludin levels in intestinal mucosa	899:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	7	6	theme	IL-6	875:878	arg1	levels					934:939	increased SIgA, ZO-1, and occludin levels	899:939	increased SIgA, ZO-1, and occludin levels in intestinal mucosa	899:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	7	6	theme	IL-6	875:878	arg1	levels					865:870	the levels	861:870	the levels of IL-6, LPS, and DAO	861:892	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	12	7	theme	mucosal	1520:1526	arg1	barrier					1528:1534	the intestinal mucosal barrier	1505:1534	the intestinal mucosal barrier	1505:1534	In conclusion, GQD may treat diarrhea and restore the intestinal mucosal barrier by facilitating Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB signaling pathway.
36152417	8	8	theme	Lactobacillus	1120:1132	arg1	compositions					1041:1052	the microbial compositions	1027:1052	the microbial compositions inhabited on the intestinal mucosa	1027:1087	GQD modulated the microbial compositions inhabited on the intestinal mucosa, especially an increase of the Lactobacillus.
36152417	8	8	theme	Lactobacillus	1120:1132	arg1	increase					1104:1111	an increase	1101:1111	an increase of the Lactobacillus	1101:1132	GQD modulated the microbial compositions inhabited on the intestinal mucosa, especially an increase of the Lactobacillus.
36152417	11	9	theme	TLR2	1388:1391	arg1	expressions					1373:1383	the expressions	1369:1383	the expressions of TLR2, MyD88, and NF-κB in the colon	1369:1422	Moreover, the expressions of TLR2, MyD88, and NF-κB in the colon decreased after GQD treatment.
36152417	7	10	theme	ZO-1	915:918	arg1	levels					934:939	increased SIgA, ZO-1, and occludin levels	899:939	increased SIgA, ZO-1, and occludin levels in intestinal mucosa	899:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	2	11	theme	intestinal	302:311	arg1	mucosa					313:318	the intestinal mucosa	298:318	the intestinal mucosa	298:318	The microecology inhabiting the intestinal mucosa is the key factor leading to diarrhea.
36152417	12	12	theme	intestinal	1509:1518	arg1	barrier					1528:1534	the intestinal mucosal barrier	1505:1534	the intestinal mucosal barrier	1505:1534	In conclusion, GQD may treat diarrhea and restore the intestinal mucosal barrier by facilitating Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB signaling pathway.
36152417	3	13	theme	Gegen	359:363	arg1	GQD					384:386	GQD	384:386	GQD	384:386	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	3	13	theme	Gegen	359:363	arg1	decoction					373:381	Gegen Qinlian decoction	359:381	Gegen Qinlian decoction (GQD)	359:387	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	0	14	from	barrier	48:54	arg1	piglets					78:84	bacterial diarrhea piglets	59:84	bacterial diarrhea piglets	59:84	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	7	15	theme	LPS	881:883	arg1	levels					934:939	increased SIgA, ZO-1, and occludin levels	899:939	increased SIgA, ZO-1, and occludin levels in intestinal mucosa	899:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	7	15	theme	LPS	881:883	arg1	levels					865:870	the levels	861:870	the levels of IL-6, LPS, and DAO	861:892	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	7	16	from	levels	934:939	arg1	mucosa					955:960	intestinal mucosa	944:960	intestinal mucosa	944:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	3	17	theme	Qinlian	365:371	arg1	GQD					384:386	GQD	384:386	GQD	384:386	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	3	17	theme	Qinlian	365:371	arg1	decoction					373:381	Gegen Qinlian decoction	359:381	Gegen Qinlian decoction (GQD)	359:387	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	0	18	theme	intestinal	37:46	arg1	barrier					48:54	the intestinal barrier	33:54	the intestinal barrier in bacterial diarrhea piglets	33:84	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	7	19	theme	SIgA	909:912	arg1	levels					934:939	increased SIgA, ZO-1, and occludin levels	899:939	increased SIgA, ZO-1, and occludin levels in intestinal mucosa	899:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	0	20	theme	Gegen	0:4	arg1	decoction					14:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	11	21	theme	MyD88	1394:1398	arg1	expressions					1373:1383	the expressions	1369:1383	the expressions of TLR2, MyD88, and NF-κB in the colon	1369:1422	Moreover, the expressions of TLR2, MyD88, and NF-κB in the colon decreased after GQD treatment.
36152417	0	22	theme	Qinlian	6:12	arg1	decoction					14:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	5	23	theme	piglet	617:622	arg1	model					624:628	A Tibetan piglet model	607:628	A Tibetan piglet model with bacterial diarrhea	607:652	A Tibetan piglet model with bacterial diarrhea was established through orally administered Escherichia coli, and diarrheal piglets were treated with GQD for three days.
36152417	9	24	theme	key	1187:1189	arg1	Lactobacillus					1165:1177	Lactobacillus	1165:1177	Lactobacillus	1165:1177	Spearman analysis showed that Lactobacillus was the key genus of intestinal barrier-related bacteria.
36152417	9	24	theme	key	1187:1189	arg1	genus					1191:1195	the key genus	1183:1195	the key genus of intestinal barrier-related bacteria	1183:1234	Spearman analysis showed that Lactobacillus was the key genus of intestinal barrier-related bacteria.
36152417	1	25	theme	severe	195:200	arg1	problem					209:215	a severe global problem	193:215	a severe global problem with a particularly high incidence rate in children	193:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	1	25	theme	severe	195:200	arg1	diarrhea					181:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	3	26	used	used	392:395	arg2	GQD					384:386	GQD	384:386	GQD	384:386	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	3	26	used	used	392:395	arg2	decoction					373:381	Gegen Qinlian decoction	359:381	Gegen Qinlian decoction (GQD)	359:387	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	12	27	theme	signaling	1609:1617	arg1	pathway					1619:1625	the TLR2/MyD88/NF-κB signaling pathway	1588:1625	the TLR2/MyD88/NF-κB signaling pathway	1588:1625	In conclusion, GQD may treat diarrhea and restore the intestinal mucosal barrier by facilitating Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB signaling pathway.
36152417	2	28	theme	key	327:329	arg1	microecology					274:285	The microecology	270:285	The microecology inhabiting the intestinal mucosa	270:318	The microecology inhabiting the intestinal mucosa is the key factor leading to diarrhea.
36152417	2	28	theme	key	327:329	arg1	factor					331:336	the key factor	323:336	the key factor leading to diarrhea	323:356	The microecology inhabiting the intestinal mucosa is the key factor leading to diarrhea.
36152417	5	29	theme	diarrheal	720:728	arg1	piglets					730:736	diarrheal piglets	720:736	diarrheal piglets	720:736	A Tibetan piglet model with bacterial diarrhea was established through orally administered Escherichia coli, and diarrheal piglets were treated with GQD for three days.
36152417	1	30	theme	global	202:207	arg1	problem					209:215	a severe global problem	193:215	a severe global problem with a particularly high incidence rate in children	193:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	1	30	theme	global	202:207	arg1	diarrhea					181:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	4	31	theme	GQD	537:539	arg1	effect					527:532	the restorative effect	511:532	the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota	511:604	Thus, this study aimed to clarify the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota.
36152417	7	32	theme	increased	899:907	arg1	levels					934:939	increased SIgA, ZO-1, and occludin levels	899:939	increased SIgA, ZO-1, and occludin levels in intestinal mucosa	899:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	4	33	theme	intestinal	548:557	arg1	barrier					559:565	the intestinal barrier	544:565	the intestinal barrier from the perspective of gut microbiota	544:604	Thus, this study aimed to clarify the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota.
36152417	4	34	theme	gut	591:593	arg1	microbiota					595:604	gut microbiota	591:604	gut microbiota	591:604	Thus, this study aimed to clarify the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota.
36152417	9	35	theme	intestinal	1200:1209	arg1	bacteria					1227:1234	intestinal barrier-related bacteria	1200:1234	intestinal barrier-related bacteria	1200:1234	Spearman analysis showed that Lactobacillus was the key genus of intestinal barrier-related bacteria.
36152417	10	36	theme	Lactobacillus	1301:1313	arg1	growth					1315:1320	Lactobacillus growth	1301:1320	Lactobacillus growth	1301:1320	Bacterial culture in vitro validated that GQD directly promoted Lactobacillus growth and inhibited E. coli proliferation.
36152417	10	37	dep	E.	1336:1337	arg1	coli					1339:1342	coli	1339:1342	coli	1339:1342	Bacterial culture in vitro validated that GQD directly promoted Lactobacillus growth and inhibited E. coli proliferation.
36152417	3	38	theme	bacterial	406:414	arg1	diarrhea					416:423	bacterial diarrhea	406:423	bacterial diarrhea	406:423	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	1	39	with	problem	209:215	arg1	rate					252:255	a particularly high incidence rate	222:255	a particularly high incidence rate in children	222:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	9	40	theme	barrier-related	1211:1225	arg1	bacteria					1227:1234	intestinal barrier-related bacteria	1200:1234	intestinal barrier-related bacteria	1200:1234	Spearman analysis showed that Lactobacillus was the key genus of intestinal barrier-related bacteria.
36152417	11	41	theme	GQD	1440:1442	arg1	treatment					1444:1452	GQD treatment	1440:1452	GQD treatment	1440:1452	Moreover, the expressions of TLR2, MyD88, and NF-κB in the colon decreased after GQD treatment.
36152417	3	42	theme	underlying	439:448	arg1	mechanism					450:458	its underlying mechanism	435:458	its underlying mechanism	435:458	Gegen Qinlian decoction (GQD) is used to treat bacterial diarrhea, however, its underlying mechanism remains unclear.
36152417	4	43	from	effect	527:532	arg1	barrier					559:565	the intestinal barrier	544:565	the intestinal barrier from the perspective of gut microbiota	544:604	Thus, this study aimed to clarify the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota.
36152417	4	44	theme	restorative	515:525	arg1	effect					527:532	the restorative effect	511:532	the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota	511:604	Thus, this study aimed to clarify the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota.
36152417	0	45	theme	TLR2/MyD88/NF-κB	139:154	arg1	pathway					156:162	the TLR2/MyD88/NF-κB pathway	135:162	the TLR2/MyD88/NF-κB pathway	135:162	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	9	46	theme	bacteria	1227:1234	arg1	Lactobacillus					1165:1177	Lactobacillus	1165:1177	Lactobacillus	1165:1177	Spearman analysis showed that Lactobacillus was the key genus of intestinal barrier-related bacteria.
36152417	9	46	theme	bacteria	1227:1234	arg1	genus					1191:1195	the key genus	1183:1195	the key genus of intestinal barrier-related bacteria	1183:1234	Spearman analysis showed that Lactobacillus was the key genus of intestinal barrier-related bacteria.
36152417	12	47	theme	TLR2/MyD88/NF-κB	1592:1607	arg1	pathway					1619:1625	the TLR2/MyD88/NF-κB signaling pathway	1588:1625	the TLR2/MyD88/NF-κB signaling pathway	1588:1625	In conclusion, GQD may treat diarrhea and restore the intestinal mucosal barrier by facilitating Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB signaling pathway.
36152417	4	48	from	perspective	576:586	arg1	barrier					559:565	the intestinal barrier	544:565	the intestinal barrier from the perspective of gut microbiota	544:604	Thus, this study aimed to clarify the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota.
36152417	1	49	from	rate	252:255	arg1	children					260:267	children	260:267	children	260:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	11	50	theme	NF-κB	1405:1409	arg1	expressions					1373:1383	the expressions	1369:1383	the expressions of TLR2, MyD88, and NF-κB in the colon	1369:1422	Moreover, the expressions of TLR2, MyD88, and NF-κB in the colon decreased after GQD treatment.
36152417	8	51	theme	intestinal	1071:1080	arg1	mucosa					1082:1087	the intestinal mucosa	1067:1087	the intestinal mucosa	1067:1087	GQD modulated the microbial compositions inhabited on the intestinal mucosa, especially an increase of the Lactobacillus.
36152417	11	52	from	expressions	1373:1383	arg1	colon					1418:1422	the colon	1414:1422	the colon	1414:1422	Moreover, the expressions of TLR2, MyD88, and NF-κB in the colon decreased after GQD treatment.
36152417	5	53	theme	Tibetan	609:615	arg1	model					624:628	A Tibetan piglet model	607:628	A Tibetan piglet model with bacterial diarrhea	607:652	A Tibetan piglet model with bacterial diarrhea was established through orally administered Escherichia coli, and diarrheal piglets were treated with GQD for three days.
36152417	10	54	theme	E.	1336:1337	arg1	proliferation					1344:1356	E. coli proliferation	1336:1356	E. coli proliferation	1336:1356	Bacterial culture in vitro validated that GQD directly promoted Lactobacillus growth and inhibited E. coli proliferation.
36152417	5	55	with	model	624:628	arg1	diarrhea					645:652	bacterial diarrhea	635:652	bacterial diarrhea	635:652	A Tibetan piglet model with bacterial diarrhea was established through orally administered Escherichia coli, and diarrheal piglets were treated with GQD for three days.
36152417	7	56	theme	DAO	890:892	arg1	levels					934:939	increased SIgA, ZO-1, and occludin levels	899:939	increased SIgA, ZO-1, and occludin levels in intestinal mucosa	899:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	7	56	theme	DAO	890:892	arg1	levels					865:870	the levels	861:870	the levels of IL-6, LPS, and DAO	861:892	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	1	57	theme	high	237:240	arg1	rate					252:255	a particularly high incidence rate	222:255	a particularly high incidence rate in children	222:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	7	58	theme	intestinal	993:1002	arg1	barrier					1004:1010	intestinal barrier	993:1010	intestinal barrier	993:1010	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	6	59	theme	diarrheal	827:835	arg1	symptoms					837:844	the diarrheal symptoms	823:844	the diarrheal symptoms	823:844	After treatment, GQD significantly ameliorated the diarrheal symptoms.
36152417	7	60	theme	intestinal	944:953	arg1	mucosa					955:960	intestinal mucosa	944:960	intestinal mucosa	944:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	1	61	theme	incidence	242:250	arg1	rate					252:255	a particularly high incidence rate	222:255	a particularly high incidence rate in children	222:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	0	62	theme	diarrhea	69:76	arg1	piglets					78:84	bacterial diarrhea piglets	59:84	bacterial diarrhea piglets	59:84	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	10	63	theme	Bacterial	1237:1245	arg1	culture					1247:1253	Bacterial culture	1237:1253	Bacterial culture in vitro	1237:1262	Bacterial culture in vitro validated that GQD directly promoted Lactobacillus growth and inhibited E. coli proliferation.
36152417	12	64	theme	Lactobacillus	1552:1564	arg1	growth					1566:1571	Lactobacillus growth	1552:1571	Lactobacillus growth	1552:1571	In conclusion, GQD may treat diarrhea and restore the intestinal mucosal barrier by facilitating Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB signaling pathway.
36152417	0	65	theme	bacterial	59:67	arg1	piglets					78:84	bacterial diarrhea piglets	59:84	bacterial diarrhea piglets	59:84	Gegen Qinlian decoction restores the intestinal barrier in bacterial diarrhea piglets by promoting Lactobacillus growth and inhibiting the TLR2/MyD88/NF-κB pathway.
36152417	7	66	from	levels	865:870	arg1	mucosa					955:960	intestinal mucosa	944:960	intestinal mucosa	944:960	GQD decreased the levels of IL-6, LPS, and DAO, and increased SIgA, ZO-1, and occludin levels in intestinal mucosa, indicating the restoration of intestinal barrier.
36152417	9	67	theme	Spearman	1135:1142	arg1	analysis					1144:1151	Spearman analysis	1135:1151	Spearman analysis	1135:1151	Spearman analysis showed that Lactobacillus was the key genus of intestinal barrier-related bacteria.
36152417	1	68	theme	Acute	165:169	arg1	problem					209:215	a severe global problem	193:215	a severe global problem with a particularly high incidence rate in children	193:267	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	1	68	theme	Acute	165:169	arg1	diarrhea					181:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea	165:188	Acute bacterial diarrhea is a severe global problem with a particularly high incidence rate in children.
36152417	4	69	theme	microbiota	595:604	arg1	perspective					576:586	the perspective	572:586	the perspective of gut microbiota	572:604	Thus, this study aimed to clarify the restorative effect of GQD on the intestinal barrier from the perspective of gut microbiota.
35133390	11	0	theme	layer-related	1910:1922	arg1	diseases					1924:1931	mucus layer-related diseases	1904:1931	mucus layer-related diseases such as ulcerative colitis	1904:1958	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	11	0	theme	layer-related	1910:1922	arg1	colitis					1952:1958	ulcerative colitis	1941:1958	ulcerative colitis	1941:1958	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	4	1	theme	last	717:720	arg1	days					728:731	the last seven days	713:731	the last seven days of treatment	713:744	C57BL/6 mice were gavaged with Amuc_2109 for 21 days, and during the last seven days of treatment, they drank DSS dissolved in their drinking water to induce colitis.
35133390	2	2	theme	therapeutic	383:393	arg1	effect					395:400	a significant alleviative or therapeutic effect	354:400	a significant alleviative or therapeutic effect on host enteritis	354:418	Akkermansia muciniphila is a probiotic that exerts a significant alleviative or therapeutic effect on host enteritis.
35133390	0	3	theme	sodium-induced	99:112	arg1	colitis					114:120	dextran sulfate sodium-induced colitis	83:120	dextran sulfate sodium-induced colitis in mice	83:128	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	9	4	theme	catalytic	1652:1660	arg1	acids					1668:1672	its key catalytic amino acids	1644:1672	its key catalytic amino acids	1644:1672	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	5	5	theme	lowered	915:921	arg1	scores					952:957	lowered disease activity index (DAI) scores	915:957	lowered disease activity index (DAI) scores	915:957	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	1	6	theme	Inflammatory	193:204	arg1	IBD					221:223	IBD	221:223	IBD	221:223	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	1	6	theme	Inflammatory	193:204	arg1	disease					212:218	Inflammatory bowel disease	193:218	Inflammatory bowel disease (IBD)	193:224	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	1	7	theme	gut	278:280	arg1	composition					259:269	the microbial composition	245:269	the microbial composition of the gut and its metabolites	245:300	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	6	8	theme	cytokines	1105:1113	arg1	domain					1161:1166	the NLR family pyrin domain	1140:1166	the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis	1140:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	8	theme	cytokines	1105:1113	arg1	overexpression					1074:1087	the overexpression	1070:1087	the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6)	1070:1134	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	8	theme	cytokines	1105:1113	arg1	cytokines					1105:1113	inflammatory cytokines	1092:1113	inflammatory cytokines (TNF-α, IL-1β, IL-6)	1092:1134	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	7	9	theme	proteins	1322:1329	arg1	expression					1293:1302	the mRNA expression	1284:1302	the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1)	1284:1357	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	9	10	theme	amino	1662:1666	arg1	acids					1668:1672	its key catalytic amino acids	1644:1672	its key catalytic amino acids	1644:1672	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	8	11	theme	microbial	1386:1394	arg1	sequences					1405:1413	fecal microbial 16S rRNA sequences	1380:1413	fecal microbial 16S rRNA sequences	1380:1413	Further analysis of fecal microbial 16S rRNA sequences showed that Amuc_2109 reshaped the intestinal microbiota.
35133390	5	12	theme	weight	968:973	arg1	loss					975:978	weight loss	968:978	weight loss	968:978	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	9	13	theme	wild-type	1552:1560	arg1	protein					1562:1568	the wild-type protein	1548:1568	the wild-type protein	1548:1568	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	10	14	theme	ulcerative	1797:1806	arg1	colitis					1808:1814	ulcerative colitis	1797:1814	ulcerative colitis	1797:1814	In summary, these findings demonstrate the potential of Amuc_2109, as a therapeutic agent for ulcerative colitis.
35133390	7	15	with	supplementation	1239:1253	arg1	Amuc_2109					1260:1268	Amuc_2109	1260:1268	Amuc_2109	1260:1268	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	11	16	theme	mucus	1904:1908	arg1	diseases					1924:1931	mucus layer-related diseases	1904:1931	mucus layer-related diseases such as ulcerative colitis	1904:1958	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	11	16	theme	mucus	1904:1908	arg1	colitis					1952:1958	ulcerative colitis	1941:1958	ulcerative colitis	1941:1958	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	2	17	theme	Akkermansia	303:313	arg1	muciniphila					315:325	Akkermansia muciniphila	303:325	Akkermansia muciniphila	303:325	Akkermansia muciniphila is a probiotic that exerts a significant alleviative or therapeutic effect on host enteritis.
35133390	2	17	theme	Akkermansia	303:313	arg1	probiotic					332:340	probiotic	332:340	probiotic	332:340	Akkermansia muciniphila is a probiotic that exerts a significant alleviative or therapeutic effect on host enteritis.
35133390	11	18	theme	additional	1847:1856	arg1	assistance					1858:1867	additional assistance	1847:1867	additional assistance	1847:1867	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	5	19	theme	activity	931:938	arg1	index					940:944	disease activity index	923:944	lowered disease activity index (DAI) scores	915:957	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	5	19	theme	activity	931:938	arg1	DAI					947:949	DAI	947:949	DAI	947:949	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	4	20	theme	drinking	781:788	arg1	water					790:794	their drinking water to induce colitis	775:812	their drinking water to induce colitis	775:812	C57BL/6 mice were gavaged with Amuc_2109 for 21 days, and during the last seven days of treatment, they drank DSS dissolved in their drinking water to induce colitis.
35133390	1	21	theme	bowel	206:210	arg1	IBD					221:223	IBD	221:223	IBD	221:223	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	1	21	theme	bowel	206:210	arg1	disease					212:218	Inflammatory bowel disease	193:218	Inflammatory bowel disease (IBD)	193:224	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	4	22	dep	drank	752:756	arg1	dissolved					762:770	dissolved	762:770	drank DSS dissolved in their drinking water to induce colitis	752:812	C57BL/6 mice were gavaged with Amuc_2109 for 21 days, and during the last seven days of treatment, they drank DSS dissolved in their drinking water to induce colitis.
35133390	0	23	from	muciniphila	54:64	arg1	Amuc_2109					27:35	A β-N-acetylhexosaminidase Amuc_2109	0:35	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila	0:64	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	6	24	theme	family	1148:1153	arg1	domain					1161:1166	the NLR family pyrin domain	1140:1166	the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis	1140:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	24	theme	family	1148:1153	arg1	cytokines					1105:1113	inflammatory cytokines	1092:1113	inflammatory cytokines (TNF-α, IL-1β, IL-6)	1092:1134	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	25	theme	inflammatory	1092:1103	arg1	cytokines					1105:1113	inflammatory cytokines	1092:1113	inflammatory cytokines (TNF-α, IL-1β, IL-6)	1092:1134	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	5	26	theme	DSS-induced	879:889	arg1	colitis					891:897	DSS-induced colitis	879:897	DSS-induced colitis	879:897	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	0	27	theme	β-N-acetylhexosaminidase	2:25	arg1	Amuc_2109					27:35	A β-N-acetylhexosaminidase Amuc_2109	0:35	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila	0:64	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	8	28	theme	16S	1396:1398	arg1	sequences					1405:1413	fecal microbial 16S rRNA sequences	1380:1413	fecal microbial 16S rRNA sequences	1380:1413	Further analysis of fecal microbial 16S rRNA sequences showed that Amuc_2109 reshaped the intestinal microbiota.
35133390	11	29	theme	diseases	1924:1931	arg1	prevention					1876:1885	prevention	1876:1885	prevention	1876:1885	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	11	29	theme	diseases	1924:1931	arg1	treatment					1891:1899	treatment	1891:1899	treatment	1891:1899	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	6	30	theme	NLR	1144:1146	arg1	domain					1161:1166	the NLR family pyrin domain	1140:1166	the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis	1140:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	30	theme	NLR	1144:1146	arg1	cytokines					1105:1113	inflammatory cytokines	1092:1113	inflammatory cytokines (TNF-α, IL-1β, IL-6)	1092:1134	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	7	31	theme	tight	1307:1311	arg1	proteins					1322:1329	tight junction proteins	1307:1329	tight junction proteins (ZO-1, occludin, claudin-1)	1307:1357	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	1	32	theme	metabolites	290:300	arg1	composition					259:269	the microbial composition	245:269	the microbial composition of the gut and its metabolites	245:300	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	9	33	theme	anti-inflammatory	1575:1591	arg1	effects					1593:1599	the anti-inflammatory effects	1571:1599	the anti-inflammatory effects	1571:1599	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	11	34	dep	prevention	1876:1885	arg1	the					1872:1874	the	1872:1874	the	1872:1874	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	2	35	theme	host	405:408	arg1	enteritis					410:418	host enteritis	405:418	host enteritis	405:418	Akkermansia muciniphila is a probiotic that exerts a significant alleviative or therapeutic effect on host enteritis.
35133390	2	36	from	effect	395:400	arg1	enteritis					410:418	host enteritis	405:418	host enteritis	405:418	Akkermansia muciniphila is a probiotic that exerts a significant alleviative or therapeutic effect on host enteritis.
35133390	8	37	theme	Further	1360:1366	arg1	analysis					1368:1375	Further analysis	1360:1375	Further analysis of fecal microbial 16S rRNA sequences	1360:1413	Further analysis of fecal microbial 16S rRNA sequences showed that Amuc_2109 reshaped the intestinal microbiota.
35133390	11	38	theme	ulcerative	1941:1950	arg1	colitis					1952:1958	ulcerative colitis	1941:1958	ulcerative colitis	1941:1958	We posit that it will provide additional assistance in the prevention and treatment of mucus layer-related diseases such as ulcerative colitis.
35133390	0	39	theme	intestinal	143:152	arg1	barrier					154:160	intestinal barrier	143:160	intestinal barrier	143:160	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	0	40	from	colitis	114:120	arg1	mice					125:128	mice	125:128	mice	125:128	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	5	41	theme	disease	923:929	arg1	index					940:944	disease activity index	923:944	lowered disease activity index (DAI) scores	915:957	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	5	41	theme	disease	923:929	arg1	DAI					947:949	DAI	947:949	DAI	947:949	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	3	42	from	colitis	631:637	arg1	mice					642:645	mice	642:645	mice	642:645	This study was designed to determine the protective effect and potential mechanism underlying the secretion of β-acetylaminohexosidase (Amuc_2109) by A. muciniphila against dextran sulfate sodium (DSS)-induced colitis in mice.
35133390	9	43	theme	Amuc_2109	1512:1520	arg1	effects					1501:1507	the anti-inflammatory effects	1479:1507	the anti-inflammatory effects of Amuc_2109	1479:1520	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	7	44	theme	mRNA	1288:1291	arg1	expression					1293:1302	the mRNA expression	1284:1302	the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1)	1284:1357	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	2	45	theme	significant	356:366	arg1	effect					395:400	a significant alleviative or therapeutic effect	354:400	a significant alleviative or therapeutic effect on host enteritis	354:418	Akkermansia muciniphila is a probiotic that exerts a significant alleviative or therapeutic effect on host enteritis.
35133390	9	46	theme	anti-inflammatory	1483:1499	arg1	effects					1501:1507	the anti-inflammatory effects	1479:1507	the anti-inflammatory effects of Amuc_2109	1479:1520	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	6	47	dep	cytokines	1105:1113	arg1	IL-1β					1123:1127	IL-1β	1123:1127	IL-1β	1123:1127	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	47	dep	cytokines	1105:1113	arg1	TNF-α					1116:1120	TNF-α	1116:1120	TNF-α	1116:1120	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	47	dep	cytokines	1105:1113	arg1	IL-6					1130:1133	IL-6	1130:1133	IL-6	1130:1133	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	7	48	theme	junction	1313:1320	arg1	proteins					1322:1329	tight junction proteins	1307:1329	tight junction proteins (ZO-1, occludin, claudin-1)	1307:1357	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	9	49	theme	key	1648:1650	arg1	acids					1668:1672	its key catalytic amino acids	1644:1672	its key catalytic amino acids	1644:1672	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	3	50	theme	protective	462:471	arg1	effect					473:478	the protective effect	458:478	the protective effect	458:478	This study was designed to determine the protective effect and potential mechanism underlying the secretion of β-acetylaminohexosidase (Amuc_2109) by A. muciniphila against dextran sulfate sodium (DSS)-induced colitis in mice.
35133390	5	51	with	supplementation	839:853	arg1	Amuc_2109					860:868	Amuc_2109	860:868	Amuc_2109	860:868	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	5	52	theme	index	940:944	arg1	scores					952:957	lowered disease activity index (DAI) scores	915:957	lowered disease activity index (DAI) scores	915:957	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	3	53	theme	potential	484:492	arg1	mechanism					494:502	potential mechanism	484:502	potential mechanism underlying the secretion of β-acetylaminohexosidase (Amuc_2109) by A. muciniphila against dextran sulfate sodium (DSS)-induced colitis in mice	484:645	This study was designed to determine the protective effect and potential mechanism underlying the secretion of β-acetylaminohexosidase (Amuc_2109) by A. muciniphila against dextran sulfate sodium (DSS)-induced colitis in mice.
35133390	7	54	dep	proteins	1322:1329	arg1	occludin					1338:1345	occludin	1338:1345	occludin	1338:1345	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	7	54	dep	proteins	1322:1329	arg1	ZO-1					1332:1335	ZO-1	1332:1335	ZO-1	1332:1335	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	7	54	dep	proteins	1322:1329	arg1	claudin-1					1348:1356	claudin-1	1348:1356	claudin-1	1348:1356	Furthermore, supplementation with Amuc_2109 also restored the mRNA expression of tight junction proteins (ZO-1, occludin, claudin-1).
35133390	2	55	theme	alleviative	368:378	arg1	effect					395:400	a significant alleviative or therapeutic effect	354:400	a significant alleviative or therapeutic effect on host enteritis	354:418	Akkermansia muciniphila is a probiotic that exerts a significant alleviative or therapeutic effect on host enteritis.
35133390	6	56	theme	DSS-induced	1205:1215	arg1	colitis					1217:1223	DSS-induced colitis	1205:1223	DSS-induced colitis	1205:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	0	57	theme	gut	177:179	arg1	microbiota					181:190	gut microbiota	177:190	gut microbiota	177:190	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	3	58	theme	-induced	622:629	arg1	colitis					631:637	dextran sulfate sodium (DSS)-induced colitis	594:637	dextran sulfate sodium (DSS)-induced colitis in mice	594:645	This study was designed to determine the protective effect and potential mechanism underlying the secretion of β-acetylaminohexosidase (Amuc_2109) by A. muciniphila against dextran sulfate sodium (DSS)-induced colitis in mice.
35133390	9	59	theme	acids	1668:1672	arg1	mutation					1632:1639	the mutation	1628:1639	the mutation of its key catalytic amino acids	1628:1672	While the anti-inflammatory effects of Amuc_2109 were only manifested with the wild-type protein, the anti-inflammatory effects were completely lost after the mutation of its key catalytic amino acids rendered Amuc_2109 inactive.
35133390	3	60	theme	β-acetylaminohexosidase	532:554	arg1	secretion					519:527	the secretion	515:527	the secretion of β-acetylaminohexosidase (Amuc_2109) by A. muciniphila against dextran sulfate sodium (DSS)-induced colitis in mice	515:645	This study was designed to determine the protective effect and potential mechanism underlying the secretion of β-acetylaminohexosidase (Amuc_2109) by A. muciniphila against dextran sulfate sodium (DSS)-induced colitis in mice.
35133390	10	61	theme	therapeutic	1775:1785	arg1	agent					1787:1791	a therapeutic agent	1773:1791	a therapeutic agent for ulcerative colitis	1773:1814	In summary, these findings demonstrate the potential of Amuc_2109, as a therapeutic agent for ulcerative colitis.
35133390	10	61	theme	therapeutic	1775:1785	arg1	potential					1746:1754	the potential	1742:1754	the potential of Amuc_2109	1742:1767	In summary, these findings demonstrate the potential of Amuc_2109, as a therapeutic agent for ulcerative colitis.
35133390	8	62	theme	sequences	1405:1413	arg1	analysis					1368:1375	Further analysis	1360:1375	Further analysis of fecal microbial 16S rRNA sequences	1360:1413	Further analysis of fecal microbial 16S rRNA sequences showed that Amuc_2109 reshaped the intestinal microbiota.
35133390	8	63	theme	rRNA	1400:1403	arg1	sequences					1405:1413	fecal microbial 16S rRNA sequences	1380:1413	fecal microbial 16S rRNA sequences	1380:1413	Further analysis of fecal microbial 16S rRNA sequences showed that Amuc_2109 reshaped the intestinal microbiota.
35133390	5	64	theme	colon	991:995	arg1	length					997:1002	colon length	991:1002	colon length	991:1002	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	5	65	theme	oxidative	1019:1027	arg1	stress					1029:1034	oxidative stress	1019:1034	oxidative stress	1019:1034	Our results showed that supplementation with Amuc_2109 improved DSS-induced colitis as evidenced by lowered disease activity index (DAI) scores, reduced weight loss, increased colon length, and inhibited oxidative stress.
35133390	4	66	theme	treatment	736:744	arg1	days					728:731	the last seven days	713:731	the last seven days of treatment	713:744	C57BL/6 mice were gavaged with Amuc_2109 for 21 days, and during the last seven days of treatment, they drank DSS dissolved in their drinking water to induce colitis.
35133390	6	67	from	inflammasome	1189:1200	arg1	colitis					1217:1223	DSS-induced colitis	1205:1223	DSS-induced colitis	1205:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	68	theme	NLRP3	1182:1186	arg1	inflammasome					1189:1200	3 (NLRP3) inflammasome	1179:1200	3 (NLRP3) inflammasome in DSS-induced colitis	1179:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	8	69	theme	intestinal	1450:1459	arg1	microbiota					1461:1470	the intestinal microbiota	1446:1470	the intestinal microbiota	1446:1470	Further analysis of fecal microbial 16S rRNA sequences showed that Amuc_2109 reshaped the intestinal microbiota.
35133390	1	70	theme	microbial	249:257	arg1	composition					259:269	the microbial composition	245:269	the microbial composition of the gut and its metabolites	245:300	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	0	71	theme	sulfate	91:97	arg1	colitis					114:120	dextran sulfate sodium-induced colitis	83:120	dextran sulfate sodium-induced colitis in mice	83:128	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	1	72	dep	gut	278:280	arg1	the					274:276	the	274:276	the	274:276	Inflammatory bowel disease (IBD) is associated with the microbial composition of the gut and its metabolites.
35133390	8	73	theme	fecal	1380:1384	arg1	sequences					1405:1413	fecal microbial 16S rRNA sequences	1380:1413	fecal microbial 16S rRNA sequences	1380:1413	Further analysis of fecal microbial 16S rRNA sequences showed that Amuc_2109 reshaped the intestinal microbiota.
35133390	6	74	theme	pyrin	1155:1159	arg1	domain					1161:1166	the NLR family pyrin domain	1140:1166	the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis	1140:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	74	theme	pyrin	1155:1159	arg1	cytokines					1105:1113	inflammatory cytokines	1092:1113	inflammatory cytokines (TNF-α, IL-1β, IL-6)	1092:1134	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	0	75	theme	dextran	83:89	arg1	sulfate					91:97	dextran sulfate	83:97	dextran sulfate sodium-induced colitis in mice	83:128	A β-N-acetylhexosaminidase Amuc_2109 from Akkermansia muciniphila protects against dextran sulfate sodium-induced colitis in mice by enhancing intestinal barrier and modulating gut microbiota.
35133390	6	76	contain	containing	1168:1177	arg1	domain					1161:1166	the NLR family pyrin domain	1140:1166	the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis	1140:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	76	contain	containing	1168:1177	arg2	inflammasome					1189:1200	3 (NLRP3) inflammasome	1179:1200	3 (NLRP3) inflammasome in DSS-induced colitis	1179:1223	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	6	76	contain	containing	1168:1177	arg1	cytokines					1105:1113	inflammatory cytokines	1092:1113	inflammatory cytokines (TNF-α, IL-1β, IL-6)	1092:1134	In addition, Amuc_2109 inhibited the overexpression of inflammatory cytokines (TNF-α, IL-1β, IL-6) and the NLR family pyrin domain containing 3 (NLRP3) inflammasome in DSS-induced colitis.
35133390	10	77	theme	Amuc_2109	1759:1767	arg1	agent					1787:1791	a therapeutic agent	1773:1791	a therapeutic agent for ulcerative colitis	1773:1814	In summary, these findings demonstrate the potential of Amuc_2109, as a therapeutic agent for ulcerative colitis.
35133390	10	77	theme	Amuc_2109	1759:1767	arg1	potential					1746:1754	the potential	1742:1754	the potential of Amuc_2109	1742:1767	In summary, these findings demonstrate the potential of Amuc_2109, as a therapeutic agent for ulcerative colitis.
35133390	4	78	theme	C57BL/6	648:654	arg1	mice					656:659	C57BL/6 mice	648:659	C57BL/6 mice	648:659	C57BL/6 mice were gavaged with Amuc_2109 for 21 days, and during the last seven days of treatment, they drank DSS dissolved in their drinking water to induce colitis.
36584572	4	0	theme	fewer	839:843	arg1	ulceration					845:854	fewer ulceration	839:854	fewer ulceration	839:854	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	6	1	from	efficacy	1199:1206	arg1	GM					1227:1228	GM	1227:1228	GM	1227:1228	Importantly, it is positive correlated between the efficacy of OMT on colitis, GM and inflammatory DCs.
36584572	6	1	from	efficacy	1199:1206	arg1	DCs					1247:1249	inflammatory DCs	1234:1249	inflammatory DCs	1234:1249	Importantly, it is positive correlated between the efficacy of OMT on colitis, GM and inflammatory DCs.
36584572	6	1	from	efficacy	1199:1206	arg1	colitis					1218:1224	colitis	1218:1224	colitis	1218:1224	Importantly, it is positive correlated between the efficacy of OMT on colitis, GM and inflammatory DCs.
36584572	4	2	theme	length	721:726	arg1	rate					660:663	the change rate	649:663	the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice	649:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	2	3	from	IBD	404:406	arg1	China					411:415	China	411:415	China	411:415	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	0	4	theme	gut	87:89	arg1	microbiota					91:100	gut microbiota	87:100	gut microbiota	87:100	Oxymatrine ameliorated experimental colitis via mechanisms involving inflammatory DCs, gut microbiota and TLR/NF-κB pathway.
36584572	4	5	theme	colon	715:719	arg1	length					721:726	colon length	715:726	colon length	715:726	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	6	theme	mice	590:593	arg1	10 days					551:557	10 days	551:557	10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS)	551:646	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	5	7	dep	DCs	1021:1023	arg1	such					1026:1029	such	1026:1029	such	1026:1029	The percents of inflammatory DCs (such as TNF-α+DCs, iNOS+DCs, CXCR5+DCs and E-cadherin+DCs) were markedly decreased, and the GM composition was regulated.
36584572	6	8	theme	inflammatory	1234:1245	arg1	DCs					1247:1249	inflammatory DCs	1234:1249	inflammatory DCs	1234:1249	Importantly, it is positive correlated between the efficacy of OMT on colitis, GM and inflammatory DCs.
36584572	0	9	theme	TLR/NF-κB	106:114	arg1	pathway					116:122	TLR/NF-κB pathway	106:122	TLR/NF-κB pathway	106:122	Oxymatrine ameliorated experimental colitis via mechanisms involving inflammatory DCs, gut microbiota and TLR/NF-κB pathway.
36584572	2	10	attach	derived	320:326	arg1	root					337:340	the root	333:340	the root of the Sophora flavescens	333:366	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	2	10	attach	derived	320:326	arg2	Oxymatrine					369:378	Oxymatrine	369:378	Oxymatrine (OMT)	369:384	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	2	10	attach	derived	320:326	arg2	constituent					308:318	a major bioactive constituent	290:318	a major bioactive constituent derived from the root of the Sophora flavescens	290:366	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	4	11	theme	DAI	729:731	arg1	score					733:737	DAI score	729:737	DAI score	729:737	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	12	theme	OMT	562:564	arg1	mice					590:593	OMT (100 mg/kg/day) treated mice	562:593	OMT (100 mg/kg/day) treated mice	562:593	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	13	theme	mice	789:792	arg1	scores					771:776	colonic pathological damage scores	743:776	colonic pathological damage scores	743:776	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	13	theme	mice	789:792	arg1	weight					673:678	body weight	668:678	body weight	668:678	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	13	theme	mice	789:792	arg1	index					708:712	colon weight index	695:712	colon weight index	695:712	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	13	theme	mice	789:792	arg1	score					733:737	DAI score	729:737	DAI score	729:737	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	13	theme	mice	789:792	arg1	weight					687:692	colon weight	681:692	colon weight	681:692	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	13	theme	mice	789:792	arg1	length					721:726	colon length	715:726	colon length	715:726	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	7	14	theme	blotting	1271:1278	arg1	assay					1280:1284	Western blotting assay	1263:1284	Western blotting assay	1263:1284	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	7	14	theme	blotting	1271:1278	arg1	Meanwhile					1252:1260	Meanwhile	1252:1260	Meanwhile	1252:1260	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	4	15	theme	IL-33	975:979	arg1	expression					906:915	the increased expression	892:915	the increased expression of IL-4 and IL-13	892:933	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	15	theme	IL-33	975:979	arg1	ulceration					845:854	fewer ulceration	839:854	fewer ulceration	839:854	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	15	theme	IL-33	975:979	arg1	expression					954:963	the decreased expression	940:963	the decreased expression of CCL-2, IL-33 and IFN-γ	940:989	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	15	theme	IL-33	975:979	arg1	infiltration					878:889	inflammatory cell infiltration	860:889	inflammatory cell infiltration	860:889	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	16	theme	weight	701:706	arg1	index					708:712	colon weight index	695:712	colon weight index	695:712	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	17	theme	TLR	1624:1626	arg1	pathway					1646:1652	the TLR / NF-κB signaling pathway	1620:1652	the TLR / NF-κB signaling pathway	1620:1652	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	4	18	theme	change	653:658	arg1	rate					660:663	the change rate	649:663	the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice	649:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	1	19	theme	gut	170:172	arg1	GM					186:187	GM	186:187	GM	186:187	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	1	19	theme	gut	170:172	arg1	microbiota					174:183	gut microbiota	170:183	gut microbiota (GM)	170:188	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	4	20	theme	colon	695:699	arg1	index					708:712	colon weight index	695:712	colon weight index	695:712	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	21	theme	IFN-γ	985:989	arg1	expression					906:915	the increased expression	892:915	the increased expression of IL-4 and IL-13	892:933	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	21	theme	IFN-γ	985:989	arg1	ulceration					845:854	fewer ulceration	839:854	fewer ulceration	839:854	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	21	theme	IFN-γ	985:989	arg1	expression					954:963	the decreased expression	940:963	the decreased expression of CCL-2, IL-33 and IFN-γ	940:989	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	21	theme	IFN-γ	985:989	arg1	infiltration					878:889	inflammatory cell infiltration	860:889	inflammatory cell infiltration	860:889	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	1	22	theme	microbiota	174:183	arg1	crosstalk					157:165	the crosstalk	153:165	the crosstalk of gut microbiota (GM) and dendritic cells (DCs)	153:214	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	1	22	theme	microbiota	174:183	arg1	critical					220:227	critical	220:227	critical	220:227	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	2	23	used	used	390:393	arg2	OMT					381:383	OMT	381:383	OMT	381:383	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	2	23	used	used	390:393	arg2	Oxymatrine					369:378	Oxymatrine	369:378	Oxymatrine (OMT)	369:384	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	2	23	used	used	390:393	arg2	constituent					308:318	a major bioactive constituent	290:318	a major bioactive constituent derived from the root of the Sophora flavescens	290:366	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	7	24	theme	signaling	1418:1426	arg1	pathway					1428:1434	the TLR / NF-κB signaling pathway	1402:1434	the TLR / NF-κB signaling pathway	1402:1434	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	7	25	theme	NF-κB	1412:1416	arg1	pathway					1428:1434	the TLR / NF-κB signaling pathway	1402:1434	the TLR / NF-κB signaling pathway	1402:1434	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	5	26	theme	inflammatory	1008:1019	arg1	DCs					1021:1023	inflammatory DCs	1008:1023	inflammatory DCs (such as TNF-α+DCs, iNOS+DCs, CXCR5+DCs and E-cadherin+DCs)	1008:1083	The percents of inflammatory DCs (such as TNF-α+DCs, iNOS+DCs, CXCR5+DCs and E-cadherin+DCs) were markedly decreased, and the GM composition was regulated.
36584572	4	27	theme	index	708:712	arg1	rate					660:663	the change rate	649:663	the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice	649:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	28	theme	DCs	1593:1595	arg1	crosstalk					1567:1575	the crosstalk	1563:1575	the crosstalk of inflammatory DCs and GM	1563:1602	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	4	29	theme	sulfate	627:633	arg1	DSS					643:645	DSS	643:645	DSS	643:645	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	29	theme	sulfate	627:633	arg1	sodium					635:640	dextran sulfate sodium	619:640	dextran sulfate sodium (DSS)	619:646	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	7	30	theme	TLR	1406:1408	arg1	pathway					1428:1434	the TLR / NF-κB signaling pathway	1402:1434	the TLR / NF-κB signaling pathway	1402:1434	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	4	31	theme	increased	896:904	arg1	expression					906:915	the increased expression	892:915	the increased expression of IL-4 and IL-13	892:933	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	1	32	theme	inflammatory	253:264	arg1	IBD					281:283	IBD	281:283	IBD	281:283	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	1	32	theme	inflammatory	253:264	arg1	disease					272:278	inflammatory bowel disease	253:278	inflammatory bowel disease (IBD)	253:284	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	4	33	theme	scores	771:776	arg1	rate					660:663	the change rate	649:663	the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice	649:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	34	theme	inflammatory	1580:1591	arg1	DCs					1593:1595	inflammatory DCs	1580:1595	inflammatory DCs	1580:1595	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	4	35	theme	decreased	944:952	arg1	expression					954:963	the decreased expression	940:963	the decreased expression of CCL-2, IL-33 and IFN-γ	940:989	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	0	36	theme	experimental	23:34	arg1	colitis					36:42	experimental colitis	23:42	experimental colitis	23:42	Oxymatrine ameliorated experimental colitis via mechanisms involving inflammatory DCs, gut microbiota and TLR/NF-κB pathway.
36584572	1	37	theme	bowel	266:270	arg1	IBD					281:283	IBD	281:283	IBD	281:283	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	1	37	theme	bowel	266:270	arg1	disease					272:278	inflammatory bowel disease	253:278	inflammatory bowel disease (IBD)	253:284	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	4	38	theme	damage	764:769	arg1	scores					771:776	colonic pathological damage scores	743:776	colonic pathological damage scores	743:776	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	39	theme	protective	1466:1475	arg1	effect					1477:1482	the protective effect	1462:1482	the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway	1462:1652	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	4	40	theme	CCL-2	968:972	arg1	expression					906:915	the increased expression	892:915	the increased expression of IL-4 and IL-13	892:933	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	40	theme	CCL-2	968:972	arg1	ulceration					845:854	fewer ulceration	839:854	fewer ulceration	839:854	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	40	theme	CCL-2	968:972	arg1	expression					954:963	the decreased expression	940:963	the decreased expression of CCL-2, IL-33 and IFN-γ	940:989	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	40	theme	CCL-2	968:972	arg1	infiltration					878:889	inflammatory cell infiltration	860:889	inflammatory cell infiltration	860:889	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	1	41	theme	disease	272:278	arg1	pathogenesis					237:248	the pathogenesis	233:248	the pathogenesis of inflammatory bowel disease (IBD)	233:284	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	4	42	theme	colitis	781:787	arg1	mice					789:792	colitis mice	781:792	colitis mice	781:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	43	theme	colon	681:685	arg1	weight					687:692	colon weight	681:692	colon weight	681:692	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	7	44	theme	TLR4	1331:1334	arg1	Myd88					1337:1341	Myd88	1337:1341	Myd88	1337:1341	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	7	44	theme	TLR4	1331:1334	arg1	activation					1317:1326	the activation	1313:1326	the activation of TLR4	1313:1334	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	1	45	theme	dendritic	194:202	arg1	DCs					211:213	DCs	211:213	DCs	211:213	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	1	45	theme	dendritic	194:202	arg1	cells					204:208	dendritic cells	194:208	dendritic cells (DCs)	194:214	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	2	46	theme	Sophora	349:355	arg1	flavescens					357:366	the Sophora flavescens	345:366	the Sophora flavescens	345:366	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	6	47	theme	OMT	1211:1213	arg1	efficacy					1199:1206	the efficacy	1195:1206	the efficacy of OMT on colitis, GM and inflammatory DCs	1195:1249	Importantly, it is positive correlated between the efficacy of OMT on colitis, GM and inflammatory DCs.
36584572	7	48	theme	Western	1263:1269	arg1	assay					1280:1284	Western blotting assay	1263:1284	Western blotting assay	1263:1284	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	7	48	theme	Western	1263:1269	arg1	Meanwhile					1252:1260	Meanwhile	1252:1260	Meanwhile	1252:1260	Meanwhile, Western blotting assay showed that OMT suppressed the activation of TLR4, Myd88, IRAK4, IRAK1, TRAF6, TAK1, TAB, MKK3, MKK6, P38, NF-κB in the TLR / NF-κB signaling pathway.
36584572	1	49	theme	cells	204:208	arg1	crosstalk					157:165	the crosstalk	153:165	the crosstalk of gut microbiota (GM) and dendritic cells (DCs)	153:214	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	1	49	theme	cells	204:208	arg1	critical					220:227	critical	220:227	critical	220:227	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	0	50	theme	inflammatory	69:80	arg1	DCs					82:84	inflammatory DCs	69:84	inflammatory DCs	69:84	Oxymatrine ameliorated experimental colitis via mechanisms involving inflammatory DCs, gut microbiota and TLR/NF-κB pathway.
36584572	5	51	theme	DCs	1021:1023	arg1	percents					996:1003	The percents	992:1003	The percents of inflammatory DCs (such as TNF-α+DCs, iNOS+DCs, CXCR5+DCs and E-cadherin+DCs)	992:1083	The percents of inflammatory DCs (such as TNF-α+DCs, iNOS+DCs, CXCR5+DCs and E-cadherin+DCs) were markedly decreased, and the GM composition was regulated.
36584572	2	52	theme	bioactive	298:306	arg1	Oxymatrine					369:378	Oxymatrine	369:378	Oxymatrine (OMT)	369:384	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	2	52	theme	bioactive	298:306	arg1	constituent					308:318	a major bioactive constituent	290:318	a major bioactive constituent derived from the root of the Sophora flavescens	290:366	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	4	53	theme	inflammatory	860:871	arg1	infiltration					878:889	inflammatory cell infiltration	860:889	inflammatory cell infiltration	860:889	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	54	theme	score	733:737	arg1	rate					660:663	the change rate	649:663	the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice	649:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	55	with	10 days	551:557	arg1	colitis					600:606	colitis	600:606	colitis induced by dextran sulfate sodium (DSS)	600:646	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	56	theme	weight	687:692	arg1	rate					660:663	the change rate	649:663	the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice	649:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	57	theme	experimental	1508:1519	arg1	colitis					1521:1527	the DSS-induced experimental colitis	1492:1527	the DSS-induced experimental colitis	1492:1527	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	2	58	theme	major	292:296	arg1	Oxymatrine					369:378	Oxymatrine	369:378	Oxymatrine (OMT)	369:384	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	2	58	theme	major	292:296	arg1	constituent					308:318	a major bioactive constituent	290:318	a major bioactive constituent derived from the root of the Sophora flavescens	290:366	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	4	59	theme	pathological	751:762	arg1	scores					771:776	colonic pathological damage scores	743:776	colonic pathological damage scores	743:776	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	60	theme	IL-4	920:923	arg1	expression					906:915	the increased expression	892:915	the increased expression of IL-4 and IL-13	892:933	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	60	theme	IL-4	920:923	arg1	ulceration					845:854	fewer ulceration	839:854	fewer ulceration	839:854	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	60	theme	IL-4	920:923	arg1	expression					954:963	the decreased expression	940:963	the decreased expression of CCL-2, IL-33 and IFN-γ	940:989	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	60	theme	IL-4	920:923	arg1	infiltration					878:889	inflammatory cell infiltration	860:889	inflammatory cell infiltration	860:889	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	61	theme	NF-κB	1630:1634	arg1	pathway					1646:1652	the TLR / NF-κB signaling pathway	1620:1652	the TLR / NF-κB signaling pathway	1620:1652	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	8	62	theme	DSS-induced	1496:1506	arg1	colitis					1521:1527	the DSS-induced experimental colitis	1492:1527	the DSS-induced experimental colitis	1492:1527	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	1	63	theme	common	131:136	arg1	knowledge					138:146	common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD)	131:284	common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD)	131:284	It is common knowledge that the crosstalk of gut microbiota (GM) and dendritic cells (DCs) are critical for the pathogenesis of inflammatory bowel disease (IBD).
36584572	4	64	theme	IL-13	929:933	arg1	expression					906:915	the increased expression	892:915	the increased expression of IL-4 and IL-13	892:933	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	64	theme	IL-13	929:933	arg1	ulceration					845:854	fewer ulceration	839:854	fewer ulceration	839:854	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	64	theme	IL-13	929:933	arg1	expression					954:963	the decreased expression	940:963	the decreased expression of CCL-2, IL-33 and IFN-γ	940:989	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	64	theme	IL-13	929:933	arg1	infiltration					878:889	inflammatory cell infiltration	860:889	inflammatory cell infiltration	860:889	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	65	theme	colonic	743:749	arg1	scores					771:776	colonic pathological damage scores	743:776	colonic pathological damage scores	743:776	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	66	theme	dextran	619:625	arg1	DSS					643:645	DSS	643:645	DSS	643:645	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	66	theme	dextran	619:625	arg1	sodium					635:640	dextran sulfate sodium	619:640	dextran sulfate sodium (DSS)	619:646	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	2	67	theme	flavescens	357:366	arg1	root					337:340	the root	333:340	the root of the Sophora flavescens	333:366	As a major bioactive constituent derived from the root of the Sophora flavescens, Oxymatrine (OMT) was used to treat IBD in China.
36584572	4	68	theme	weight	673:678	arg1	rate					660:663	the change rate	649:663	the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice	649:792	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	69	theme	treated	582:588	arg1	mice					590:593	OMT (100 mg/kg/day) treated mice	562:593	OMT (100 mg/kg/day) treated mice	562:593	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	5	70	theme	GM	1118:1119	arg1	composition					1121:1131	the GM composition	1114:1131	the GM composition	1114:1131	The percents of inflammatory DCs (such as TNF-α+DCs, iNOS+DCs, CXCR5+DCs and E-cadherin+DCs) were markedly decreased, and the GM composition was regulated.
36584572	4	71	theme	present	530:536	arg1	study					538:542	the present study	526:542	the present study	526:542	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	4	72	theme	cell	873:876	arg1	infiltration					878:889	inflammatory cell infiltration	860:889	inflammatory cell infiltration	860:889	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	73	theme	GM	1601:1602	arg1	crosstalk					1567:1575	the crosstalk	1563:1575	the crosstalk of inflammatory DCs and GM	1563:1602	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
36584572	4	74	theme	body	668:671	arg1	weight					673:678	body weight	668:678	body weight	668:678	In the present study, after 10 days of OMT (100 mg/kg/day) treated mice with colitis induced by dextran sulfate sodium (DSS), the change rate of body weight, colon weight, colon weight index, colon length, DAI score and colonic pathological damage scores of colitis mice were significantly ameliorate, followed with fewer ulceration and inflammatory cell infiltration, the increased expression of IL-4 and IL-13, and the decreased expression of CCL-2, IL-33 and IFN-γ.
36584572	8	75	theme	signaling	1636:1644	arg1	pathway					1646:1652	the TLR / NF-κB signaling pathway	1620:1652	the TLR / NF-κB signaling pathway	1620:1652	In summary, OMT exhibits the protective effect against the DSS-induced experimental colitis, which was achieved by regulating the crosstalk of inflammatory DCs and GM, and inhibiting the TLR / NF-κB signaling pathway.
37185919	0	0	from	profile	26:32	arg1	liver					68:72	murine fetal liver	55:72	murine fetal liver	55:72	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.
37185919	8	1	theme	fetal	1077:1081	arg1	liver					1083:1087	the fetal liver	1073:1087	the fetal liver	1073:1087	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	2	theme	sugar	988:992	arg1	residues					994:1001	the following sugar residues	974:1001	the following sugar residues	974:1001	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	5	3	theme	cell	600:603	arg1	states					616:621	different cell activation states	590:621	different cell activation states	590:621	In addition, carbohydrates can modulate different cell activation states.
37185919	8	4	theme	oligosaccharides	1186:1201	arg1	GlcNAc					1153:1158	GlcNAc	1153:1158	GlcNAc	1153:1158	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	4	theme	oligosaccharides	1186:1201	arg1	α-mannose					1120:1128	α-mannose	1120:1128	α-mannose	1120:1128	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	4	theme	oligosaccharides	1186:1201	arg1	α-glucose					1131:1139	α-glucose	1131:1139	α-glucose	1131:1139	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	4	theme	oligosaccharides	1186:1201	arg1	types					1169:1173	two types	1165:1173	two types of complex oligosaccharides	1165:1201	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	4	theme	oligosaccharides	1186:1201	arg1	ages					1114:1117	different gestational ages	1092:1117	different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides	1092:1201	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	4	theme	oligosaccharides	1186:1201	arg1	galactose					1142:1150	galactose	1142:1150	galactose	1142:1150	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	4	5	theme	glycosylation	506:518	arg1	patterns					520:527	glycosylation patterns	506:527	glycosylation patterns	506:527	Hematopoietic cell migration occurs through cytokine stimulation, receptor expression, and glycosylation patterns on the cell surface.
37185919	10	6	theme	alternative	1469:1479	arg1	alternative					1481:1491	a less time-consuming and robust alternative alternative	1436:1491	a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver	1436:1644	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	10	6	theme	alternative	1469:1479	arg1	lectins					1328:1334	the lectins	1324:1334	the lectins that exhibited high and specific pattern intensities at liver capsules and vessels	1324:1417	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	5	7	theme	activation	605:614	arg1	states					616:621	different cell activation states	590:621	different cell activation states	590:621	In addition, carbohydrates can modulate different cell activation states.
37185919	10	8	theme	liver	1392:1396	arg1	capsules					1398:1405	liver capsules	1392:1405	liver capsules	1392:1405	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	8	9	theme	complex	1178:1184	arg1	oligosaccharides					1186:1201	complex oligosaccharides	1178:1201	complex oligosaccharides	1178:1201	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	3	10	from	gut	359:361	arg1	budding					342:348	budding	342:348	budding from the gut, where first hematopoietic cells arrive and expand	342:412	Around E10.5, the fetal liver starts budding from the gut, where first hematopoietic cells arrive and expand.
37185919	10	11	theme	robust	1462:1467	arg1	alternative					1481:1491	a less time-consuming and robust alternative alternative	1436:1491	a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver	1436:1644	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	10	11	theme	robust	1462:1467	arg1	lectins					1328:1334	the lectins	1324:1334	the lectins that exhibited high and specific pattern intensities at liver capsules and vessels	1324:1417	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	8	12	theme	proliferating	1021:1033	arg1	megakaryocytes					1055:1068	proliferating and differentiating megakaryocytes	1021:1068	proliferating and differentiating megakaryocytes in the fetal liver	1021:1087	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	6	13	theme	fetal	715:719	arg1	liver					721:725	mouse fetal liver	709:725	mouse fetal liver according to their glycan residues at different gestational ages through lectins	709:806	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	10	14	theme	conventional	1496:1507	arg1	antibodies					1509:1518	conventional antibodies	1496:1518	conventional antibodies	1496:1518	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	8	15	theme	gestational	1102:1112	arg1	α-glucose					1131:1139	α-glucose	1131:1139	α-glucose	1131:1139	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	15	theme	gestational	1102:1112	arg1	GlcNAc					1153:1158	GlcNAc	1153:1158	GlcNAc	1153:1158	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	15	theme	gestational	1102:1112	arg1	α-mannose					1120:1128	α-mannose	1120:1128	α-mannose	1120:1128	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	15	theme	gestational	1102:1112	arg1	ages					1114:1117	different gestational ages	1092:1117	different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides	1092:1201	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	15	theme	gestational	1102:1112	arg1	galactose					1142:1150	galactose	1142:1150	galactose	1142:1150	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	9	16	theme	proliferation	1237:1249	arg1	waves					1251:1255	three proliferation waves	1231:1255	three proliferation waves	1231:1255	Megakaryocytes also showed three proliferation waves during liver development at E12.5, E14.5, and E18.5.
37185919	2	17	theme	definitive	274:283	arg1	place					285:289	their definitive place	268:289	their definitive place in adulthood	268:302	During embryonic development, these cells migrate through different organs until they reach the bone marrow, their definitive place in adulthood.
37185919	2	17	theme	definitive	274:283	arg1	marrow					260:265	the bone marrow	251:265	the bone marrow	251:265	During embryonic development, these cells migrate through different organs until they reach the bone marrow, their definitive place in adulthood.
37185919	6	18	theme	mouse	709:713	arg1	liver					721:725	mouse fetal liver	709:725	mouse fetal liver according to their glycan residues at different gestational ages through lectins	709:806	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	10	19	theme	megakaryocyte	1597:1609	arg1	differentiation					1611:1625	megakaryocyte differentiation	1597:1625	megakaryocyte differentiation in the fetal liver	1597:1644	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	8	20	theme	different	1092:1100	arg1	α-glucose					1131:1139	α-glucose	1131:1139	α-glucose	1131:1139	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	20	theme	different	1092:1100	arg1	GlcNAc					1153:1158	GlcNAc	1153:1158	GlcNAc	1153:1158	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	20	theme	different	1092:1100	arg1	α-mannose					1120:1128	α-mannose	1120:1128	α-mannose	1120:1128	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	20	theme	different	1092:1100	arg1	ages					1114:1117	different gestational ages	1092:1117	different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides	1092:1201	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	20	theme	different	1092:1100	arg1	galactose					1142:1150	galactose	1142:1150	galactose	1142:1150	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	21	theme	differentiating	1039:1053	arg1	megakaryocytes					1055:1068	proliferating and differentiating megakaryocytes	1021:1068	proliferating and differentiating megakaryocytes in the fetal liver	1021:1087	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	3	22	theme	first	370:374	arg1	cells					390:394	first hematopoietic cells	370:394	first hematopoietic cells	370:394	Around E10.5, the fetal liver starts budding from the gut, where first hematopoietic cells arrive and expand.
37185919	0	23	theme	carbohydrate	13:24	arg1	profile					26:32	Lectin-based carbohydrate profile	0:32	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development	0:91	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.
37185919	4	24	theme	receptor	481:488	arg1	expression					490:499	receptor expression	481:499	receptor expression	481:499	Hematopoietic cell migration occurs through cytokine stimulation, receptor expression, and glycosylation patterns on the cell surface.
37185919	10	25	theme	time-consuming	1443:1456	arg1	alternative					1481:1491	a less time-consuming and robust alternative alternative	1436:1491	a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver	1436:1644	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	10	25	theme	time-consuming	1443:1456	arg1	lectins					1328:1334	the lectins	1324:1334	the lectins that exhibited high and specific pattern intensities at liver capsules and vessels	1324:1417	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	0	26	theme	Lectin-based	0:11	arg1	profile					26:32	Lectin-based carbohydrate profile	0:32	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development	0:91	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.
37185919	8	27	dep	ages	1114:1117	arg1	GlcNAc					1153:1158	GlcNAc	1153:1158	GlcNAc	1153:1158	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	27	dep	ages	1114:1117	arg1	α-mannose					1120:1128	α-mannose	1120:1128	α-mannose	1120:1128	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	27	dep	ages	1114:1117	arg1	α-glucose					1131:1139	α-glucose	1131:1139	α-glucose	1131:1139	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	27	dep	ages	1114:1117	arg1	types					1169:1173	two types	1165:1173	two types of complex oligosaccharides	1165:1201	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	27	dep	ages	1114:1117	arg1	ages					1114:1117	different gestational ages	1092:1117	different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides	1092:1201	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	8	27	dep	ages	1114:1117	arg1	galactose					1142:1150	galactose	1142:1150	galactose	1142:1150	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	9	28	theme	liver	1264:1268	arg1	development					1270:1280	liver development	1264:1280	liver development	1264:1280	Megakaryocytes also showed three proliferation waves during liver development at E12.5, E14.5, and E18.5.
37185919	10	29	from	differentiation	1611:1625	arg1	liver					1640:1644	the fetal liver	1630:1644	the fetal liver	1630:1644	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	6	30	theme	megakaryocytic	685:698	arg1	cells					700:704	fetal megakaryocytic cells	679:704	fetal megakaryocytic cells	679:704	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	5	31	theme	different	590:598	arg1	states					616:621	different cell activation states	590:621	different cell activation states	590:621	In addition, carbohydrates can modulate different cell activation states.
37185919	6	32	theme	fetal	679:683	arg1	cells					700:704	fetal megakaryocytic cells	679:704	fetal megakaryocytic cells	679:704	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	6	33	from	ages	787:790	arg1	residues					753:760	their glycan residues	740:760	their glycan residues at different gestational ages through lectins	740:806	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	10	34	theme	fetal	1634:1638	arg1	liver					1640:1644	the fetal liver	1630:1644	the fetal liver	1630:1644	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	7	35	theme	Mouse	809:813	arg1	fetuses					815:821	Mouse fetuses	809:821	Mouse fetuses between E11.5 and E18.5	809:845	Mouse fetuses between E11.5 and E18.5 were formalin-fixed and, paraffin-embedded, for immunofluorescence analysis using confocal microscopy.
37185919	7	36	theme	confocal	929:936	arg1	microscopy					938:947	confocal microscopy	929:947	confocal microscopy	929:947	Mouse fetuses between E11.5 and E18.5 were formalin-fixed and, paraffin-embedded, for immunofluorescence analysis using confocal microscopy.
37185919	0	37	theme	megakaryocytes	37:50	arg1	profile					26:32	Lectin-based carbohydrate profile	0:32	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development	0:91	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.
37185919	10	38	theme	liver	1535:1539	arg1	vessels					1573:1579	vessels	1573:1579	vessels	1573:1579	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	10	38	theme	liver	1535:1539	arg1	structures					1541:1550	liver structures	1535:1550	liver structures such as capsules and vessels	1535:1579	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	10	38	theme	liver	1535:1539	arg1	capsules					1560:1567	capsules	1560:1567	capsules	1560:1567	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	4	39	theme	cytokine	459:466	arg1	stimulation					468:478	cytokine stimulation	459:478	cytokine stimulation	459:478	Hematopoietic cell migration occurs through cytokine stimulation, receptor expression, and glycosylation patterns on the cell surface.
37185919	0	40	gly	carbohydrate	13:24	arg1	megakaryocytes					37:50	megakaryocytes	37:50	megakaryocytes	37:50	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.
37185919	6	41	theme	different	765:773	arg1	ages					787:790	different gestational ages	765:790	different gestational ages through lectins	765:806	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	3	42	theme	hematopoietic	376:388	arg1	cells					390:394	first hematopoietic cells	370:394	first hematopoietic cells	370:394	Around E10.5, the fetal liver starts budding from the gut, where first hematopoietic cells arrive and expand.
37185919	2	43	theme	embryonic	166:174	arg1	development					176:186	embryonic development	166:186	embryonic development	166:186	During embryonic development, these cells migrate through different organs until they reach the bone marrow, their definitive place in adulthood.
37185919	6	44	theme	gestational	775:785	arg1	ages					787:790	different gestational ages	765:790	different gestational ages through lectins	765:806	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	0	45	theme	murine	55:60	arg1	liver					68:72	murine fetal liver	55:72	murine fetal liver	55:72	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.
37185919	2	46	theme	different	217:225	arg1	organs					227:232	different organs	217:232	different organs	217:232	During embryonic development, these cells migrate through different organs until they reach the bone marrow, their definitive place in adulthood.
37185919	10	47	theme	pattern	1369:1375	arg1	intensities					1377:1387	high and specific pattern intensities	1351:1387	high and specific pattern intensities	1351:1387	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	4	48	theme	cell	536:539	arg1	surface					541:547	the cell surface	532:547	the cell surface	532:547	Hematopoietic cell migration occurs through cytokine stimulation, receptor expression, and glycosylation patterns on the cell surface.
37185919	6	49	theme	glycan	746:751	arg1	residues					753:760	their glycan residues	740:760	their glycan residues at different gestational ages through lectins	740:806	For this reason, we aimed to characterize and quantify fetal megakaryocytic cells in mouse fetal liver according to their glycan residues at different gestational ages through lectins.
37185919	1	50	theme	blood	132:136	arg1	cells					138:142	blood cells	132:142	blood cells	132:142	Hematopoiesis is the process by which blood cells are generated.
37185919	10	51	theme	specific	1360:1367	arg1	intensities					1377:1387	high and specific pattern intensities	1351:1387	high and specific pattern intensities	1351:1387	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	4	52	theme	cell	429:432	arg1	migration					434:442	Hematopoietic cell migration	415:442	Hematopoietic cell migration	415:442	Hematopoietic cell migration occurs through cytokine stimulation, receptor expression, and glycosylation patterns on the cell surface.
37185919	8	53	theme	following	978:986	arg1	residues					994:1001	the following sugar residues	974:1001	the following sugar residues	974:1001	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	10	54	theme	high	1351:1354	arg1	intensities					1377:1387	high and specific pattern intensities	1351:1387	high and specific pattern intensities	1351:1387	Additionally, the lectins that exhibited high and specific pattern intensities at liver capsules and vessels were shown to be a less time-consuming and robust alternative alternative to conventional antibodies for displaying liver structures such as capsules and vessels, as well as for megakaryocyte differentiation in the fetal liver.
37185919	3	55	theme	fetal	323:327	arg1	liver					329:333	the fetal liver	319:333	the fetal liver	319:333	Around E10.5, the fetal liver starts budding from the gut, where first hematopoietic cells arrive and expand.
37185919	4	56	theme	Hematopoietic	415:427	arg1	migration					434:442	Hematopoietic cell migration	415:442	Hematopoietic cell migration	415:442	Hematopoietic cell migration occurs through cytokine stimulation, receptor expression, and glycosylation patterns on the cell surface.
37185919	2	57	theme	bone	255:258	arg1	place					285:289	their definitive place	268:289	their definitive place in adulthood	268:302	During embryonic development, these cells migrate through different organs until they reach the bone marrow, their definitive place in adulthood.
37185919	2	57	theme	bone	255:258	arg1	marrow					260:265	the bone marrow	251:265	the bone marrow	251:265	During embryonic development, these cells migrate through different organs until they reach the bone marrow, their definitive place in adulthood.
37185919	2	58	from	place	285:289	arg1	adulthood					294:302	adulthood	294:302	adulthood	294:302	During embryonic development, these cells migrate through different organs until they reach the bone marrow, their definitive place in adulthood.
37185919	0	59	theme	fetal	62:66	arg1	liver					68:72	murine fetal liver	55:72	murine fetal liver	55:72	Lectin-based carbohydrate profile of megakaryocytes in murine fetal liver during development.
37185919	8	60	from	megakaryocytes	1055:1068	arg1	liver					1083:1087	the fetal liver	1073:1087	the fetal liver	1073:1087	The results showed that the following sugar residues were expressed in proliferating and differentiating megakaryocytes in the fetal liver at different gestational ages: α-mannose, α-glucose, galactose, GlcNAc, and two types of complex oligosaccharides.
37185919	7	61	theme	immunofluorescence	895:912	arg1	analysis					914:921	immunofluorescence analysis	895:921	immunofluorescence analysis using confocal microscopy	895:947	Mouse fetuses between E11.5 and E18.5 were formalin-fixed and, paraffin-embedded, for immunofluorescence analysis using confocal microscopy.
35201974	7	0	theme	class	1077:1081	arg1	Alphaproteobacteria					1083:1101	the class Alphaproteobacteria	1073:1101	the class Alphaproteobacteria	1073:1101	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	9	1	theme	respiratory	1569:1579	arg1	ubiquinone-10					1593:1605	ubiquinone-10	1593:1605	ubiquinone-10	1593:1605	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	9	1	theme	respiratory	1569:1579	arg1	quinone					1581:1587	The sole respiratory quinone	1560:1587	The sole respiratory quinone	1560:1587	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	8	2	theme	strain	1419:1424	arg1	SZ9T					1426:1429	strain SZ9T	1419:1429	strain SZ9T	1419:1429	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	7	3	theme	16S	967:969	arg1	sequences					981:989	16S rRNA gene sequences	967:989	16S rRNA gene sequences	967:989	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	12	4	from	species	2313:2319	arg1	Caulobacteraceae					2352:2367	the family Caulobacteraceae	2341:2367	the family Caulobacteraceae	2341:2367	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	10	5	theme	protein-coding	1767:1780	arg1	genes					1782:1786	2769 protein-coding genes	1762:1786	2769 protein-coding genes	1762:1786	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	5	theme	protein-coding	1767:1780	arg1	genes					1797:1801	37 tRNA genes	1789:1801	37 tRNA genes	1789:1801	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	5	theme	protein-coding	1767:1780	arg1	genes					1816:1820	two rRNA genes	1807:1820	two rRNA genes	1807:1820	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	12	6	theme	name	2384:2387	arg1	nov.					2430:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	11	7	theme	family	2028:2033	arg1	Caulobacteraceae					2035:2050	the family Caulobacteraceae	2024:2050	the family Caulobacteraceae	2024:2050	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	8	theme	digital	1943:1949	arg1	hybridization					1959:1971	digital DNA-DNA hybridization	1943:1971	digital DNA-DNA hybridization	1943:1971	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	7	9	theme	Phylogenetic	936:947	arg1	analysis					949:956	Phylogenetic analysis	936:956	Phylogenetic analysis based on 16S rRNA gene sequences	936:989	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	1	10	theme	starch	163:168	arg1	supplement					149:158	supplement	149:158	supplement of starch	149:168	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	12	11	theme	saccharophilus	2410:2423	arg1	nov.					2430:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	14	12	theme	=CCTCC	2482:2487	arg1	82788T					2505:2510	=CCTCC AB2021029T=KCTC 82788T	2482:2510	=CCTCC AB2021029T=KCTC 82788T	2482:2510	The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).
35201974	14	12	theme	=CCTCC	2482:2487	arg1	SZ9T					2476:2479	SZ9T	2476:2479	SZ9T (=CCTCC AB2021029T=KCTC 82788T)	2476:2511	The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).
35201974	10	13	theme	tRNA	1792:1795	arg1	genes					1782:1786	2769 protein-coding genes	1762:1786	2769 protein-coding genes	1762:1786	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	13	theme	tRNA	1792:1795	arg1	genes					1797:1801	37 tRNA genes	1789:1801	37 tRNA genes	1789:1801	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	6	14	theme	NaCl	853:856	arg1	concentration					858:870	NaCl concentration	853:870	NaCl concentration	853:870	The range of NaCl concentration for growth was 0-1.0 % (w/v), with an optimum of 0-0.5 % (w/v).
35201974	9	15	theme	polar	1622:1626	arg1	phosphatidylcholine					1640:1658	phosphatidylcholine	1640:1658	phosphatidylcholine	1640:1658	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	9	15	theme	polar	1622:1626	arg1	lipids					1628:1633	the major polar lipids	1612:1633	the major polar lipids	1612:1633	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	10	16	theme	genomic	1831:1837	arg1	content					1843:1849	the genomic G+C content	1827:1849	the genomic G+C content	1827:1849	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	16	theme	genomic	1831:1837	arg1	%					1863:1863	41.4 mol%	1855:1863	41.4 mol%	1855:1863	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	17	theme	rRNA	1811:1814	arg1	genes					1816:1820	two rRNA genes	1807:1820	two rRNA genes	1807:1820	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	17	theme	rRNA	1811:1814	arg1	genes					1782:1786	2769 protein-coding genes	1762:1786	2769 protein-coding genes	1762:1786	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	18	theme	whole	1698:1702	arg1	genome					1704:1709	The whole genome	1694:1709	The whole genome of strain SZ9T	1694:1724	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	18	theme	whole	1698:1702	arg1	bp					1740:1741	2 842 140 bp	1730:1741	2 842 140 bp	1730:1741	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	19	theme	G+C	1839:1841	arg1	content					1843:1849	the genomic G+C content	1827:1849	the genomic G+C content	1827:1849	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	19	theme	G+C	1839:1841	arg1	%					1863:1863	41.4 mol%	1855:1863	41.4 mol%	1855:1863	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	4	20	theme	single	634:639	arg1	flagellum					647:655	a single polar flagellum	632:655	a single polar flagellum	632:655	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	3	21	dep	small	477:481	arg1	0.5-1.0 mm					498:507	0.5-1.0 mm	498:507	0.5-1.0 mm	498:507	Colonies of strain SZ9T were small (approximately 0.5-1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex.
35201974	12	22	theme	family	2345:2350	arg1	Caulobacteraceae					2352:2367	the family Caulobacteraceae	2341:2367	the family Caulobacteraceae	2341:2367	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	9	23	theme	unidentified	1668:1679	arg1	glycolipids					1681:1691	two unidentified glycolipids	1664:1691	two unidentified glycolipids	1664:1691	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	10	24	theme	SZ9T	1721:1724	arg1	genome					1704:1709	The whole genome	1694:1709	The whole genome of strain SZ9T	1694:1724	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	24	theme	SZ9T	1721:1724	arg1	bp					1740:1741	2 842 140 bp	1730:1741	2 842 140 bp	1730:1741	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	7	25	dep	Asprobacter	1272:1282	arg1	aquaticus					1284:1292	Asprobacter aquaticus DRW22-8T (89.49 %)	1272:1311	Asprobacter aquaticus DRW22-8T (89.49 %)	1272:1311	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	7	25	dep	Asprobacter	1272:1282	arg1	DRW22-8T					1294:1301	DRW22-8T	1294:1301	DRW22-8T	1294:1301	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	7	25	dep	Asprobacter	1272:1282	arg1	%					1310:1310	89.49 %	1304:1310	89.49 %	1304:1310	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	11	26	theme	average	1882:1888	arg1	identity					1901:1908	orthologous average nucleotide identity	1870:1908	orthologous average nucleotide identity	1870:1908	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	0	27	theme	gen.	36:39	arg1	nov.					41:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	2	28	theme	effluent	431:438	arg1	sample					440:445	the effluent sample	427:445	the effluent sample	427:445	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	11	29	theme	identity	1930:1937	arg1	values					1973:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	1866:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae	1866:2050	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	29	theme	identity	1930:1937	arg1	%					2102:2102	64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %	2057:2102	%	2102:2102	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	2	30	attach	isolated	413:420	arg1	sample					440:445	the effluent sample	427:445	the effluent sample	427:445	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	30	attach	isolated	413:420	arg2	strain					384:389	a novel bacterial strain	366:389	a novel bacterial strain	366:389	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	11	31	theme	average	1911:1917	arg1	identity					1930:1937	average amino acid identity	1911:1937	average amino acid identity	1911:1937	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	32	theme	%	2069:2069	arg1	values					1973:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	1866:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae	1866:2050	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	32	theme	%	2069:2069	arg1	%					2102:2102	64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %	2057:2102	%	2102:2102	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	4	33	theme	binary	684:689	arg1	fission					691:697	binary fission	684:697	binary fission	684:697	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	8	34	dep	feature	1442:1448	arg1	C16 					1464:1467	C16 	1464:1467	C16 	1464:1467	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	1	35	with	facility	135:142	arg1	supplement					149:158	supplement	149:158	supplement of starch	149:168	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	2	36	from	survey	199:204	arg1	water					256:260	ambient water	248:260	ambient water	248:260	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	36	from	survey	199:204	arg1	effluent					267:274	effluent	267:274	effluent of a water purification facility with aeration and supplement of starch as carbon source	267:363	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	36	from	survey	199:204	arg1	influent					238:245	the influent	234:245	the influent (ambient water)	234:261	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	1	37	theme	water	116:120	arg1	facility					135:142	a water purification facility	114:142	a water purification facility with supplement of starch	114:168	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	11	38	theme	identity	1901:1908	arg1	values					1973:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	1866:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae	1866:2050	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	38	theme	identity	1901:1908	arg1	%					2102:2102	64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %	2057:2102	%	2102:2102	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	6	39	theme	%	927:927	arg1	optimum					910:916	an optimum	907:916	an optimum of 0-0.5 % (w/v)	907:933	The range of NaCl concentration for growth was 0-1.0 % (w/v), with an optimum of 0-0.5 % (w/v).
35201974	11	40	theme	 and	2085:2088	arg1	values					1973:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	1866:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae	1866:2050	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	40	theme	 and	2085:2088	arg1	%					2102:2102	64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %	2057:2102	%	2102:2102	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	0	41	theme	Pseudaquidulcibacter	0:19	arg1	nov.					41:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	1	42	theme	family	75:80	arg1	Caulobacteraceae					82:97	family Caulobacteraceae	75:97	family Caulobacteraceae	75:97	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	7	43	theme	paucihalophilus	1181:1195	arg1	TH1-2T					1197:1202	Aquidulcibacter paucihalophilus TH1-2T	1165:1202	Aquidulcibacter paucihalophilus TH1-2T (92.44%)	1165:1211	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	7	43	theme	paucihalophilus	1181:1195	arg1	%					1210:1210	92.44%	1205:1210	92.44%	1205:1210	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	8	44	theme	predominant	1359:1369	arg1	acids					1377:1381	The predominant fatty acids	1355:1381	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T	1355:1429	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	44	theme	predominant	1359:1369	arg1	feature					1442:1448	summed feature 3	1435:1450	summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c)	1435:1494	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	2	45	theme	bacterial	374:382	arg1	strain					384:389	a novel bacterial strain	366:389	a novel bacterial strain	366:389	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	7	46	theme	strain	1006:1011	arg1	SZ9T					1013:1016	strain SZ9T	1006:1016	strain SZ9T	1006:1016	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	12	47	theme	phenotypic	2154:2163	arg1	analyses					2198:2205	phenotypic, chemotaxonomic and phylogenetic analyses	2154:2205	phenotypic, chemotaxonomic and phylogenetic analyses	2154:2205	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	7	48	theme	sequence	1140:1147	arg1	similarities					1149:1160	the highest 16S rRNA gene sequence similarities	1114:1160	the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%)	1114:1211	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	5	49	dep	optimum	790:796	arg1	°C					802:803	28 °C	799:803	28 °C	799:803	Growth occurred at 10-40 °C (optimum, 28 °C) and pH 5.5-8.0 (optimum, pH 7.5).
35201974	7	50	theme	gene	976:979	arg1	sequences					981:989	16S rRNA gene sequences	967:989	16S rRNA gene sequences	967:989	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	7	51	theme	rRNA	1130:1133	arg1	similarities					1149:1160	the highest 16S rRNA gene sequence similarities	1114:1160	the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%)	1114:1211	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	12	52	theme	strain	2221:2226	arg1	SZ9T					2228:2231	the isolated strain SZ9T	2208:2231	the isolated strain SZ9T	2208:2231	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	2	53	theme	communities	219:229	arg1	survey					199:204	a survey	197:204	a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source	197:363	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	54	theme	purification	287:298	arg1	facility					300:307	a water purification facility	279:307	a water purification facility with aeration and supplement of starch as carbon source	279:363	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	8	55	theme	summed	1435:1440	arg1	acids					1377:1381	The predominant fatty acids	1355:1381	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T	1355:1429	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	55	theme	summed	1435:1440	arg1	feature					1442:1448	summed feature 3	1435:1450	summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c)	1435:1494	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	10	56	from	bp	1740:1741	arg1	size					1746:1749	size	1746:1749	size	1746:1749	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	4	57	dep	anaerobic	602:610	arg1	oxidase-positive					721:736	oxidase-positive	721:736	oxidase-positive	721:736	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	4	57	dep	anaerobic	602:610	arg1	motile					613:618	motile	613:618	motile	613:618	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	4	57	dep	anaerobic	602:610	arg1	catalase-negative					742:758	catalase-negative	742:758	catalase-negative	742:758	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	4	57	dep	anaerobic	602:610	arg1	rod-shaped					658:667	rod-shaped	658:667	rod-shaped	658:667	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	4	57	dep	anaerobic	602:610	arg1	Gram-stain-negative					700:718	Gram-stain-negative	700:718	Gram-stain-negative	700:718	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	4	57	dep	anaerobic	602:610	arg1	multiplied					670:679	multiplied	670:679	multiplied	670:679	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	7	58	theme	highest	1118:1124	arg1	similarities					1149:1160	the highest 16S rRNA gene sequence similarities	1114:1160	the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%)	1114:1211	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	11	59	theme	strain	1988:1993	arg1	SZ9T					1995:1998	strain SZ9T	1988:1998	strain SZ9T	1988:1998	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	8	60	theme	total	1397:1401	arg1	acids					1409:1413	the total fatty acids	1393:1413	the total fatty acids	1393:1413	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	3	61	theme	strain	460:465	arg1	SZ9T					467:470	strain SZ9T	460:470	strain SZ9T	460:470	Colonies of strain SZ9T were small (approximately 0.5-1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex.
35201974	9	62	theme	sole	1564:1567	arg1	ubiquinone-10					1593:1605	ubiquinone-10	1593:1605	ubiquinone-10	1593:1605	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	9	62	theme	sole	1564:1567	arg1	quinone					1581:1587	The sole respiratory quinone	1560:1587	The sole respiratory quinone	1560:1587	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	8	63	theme	acids	1409:1413	arg1	acids					1409:1413	the total fatty acids	1393:1413	the total fatty acids	1393:1413	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	63	theme	acids	1409:1413	arg1	%					1388:1388	>10 %	1384:1388	>10 % of the total fatty acids	1384:1413	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	12	64	theme	gen.	2425:2428	arg1	nov.					2430:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	7	65	theme	Alphaproteobacteria	1083:1101	arg1	Caulobacteraceae					1053:1068	the family Caulobacteraceae	1042:1068	the family Caulobacteraceae of the class Alphaproteobacteria	1042:1101	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	8	66	dep	feature	1504:1510	arg1	C18 					1515:1518	C18 	1515:1518	C18 	1515:1518	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	12	67	theme	chemotaxonomic	2166:2179	arg1	analyses					2198:2205	phenotypic, chemotaxonomic and phylogenetic analyses	2154:2205	phenotypic, chemotaxonomic and phylogenetic analyses	2154:2205	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	8	68	theme	SZ9T	1426:1429	arg1	acids					1377:1381	The predominant fatty acids	1355:1381	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T	1355:1429	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	68	theme	SZ9T	1426:1429	arg1	feature					1442:1448	summed feature 3	1435:1450	summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c)	1435:1494	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	2	69	theme	ambient	248:254	arg1	water					256:260	ambient water	248:260	ambient water	248:260	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	69	theme	ambient	248:254	arg1	influent					238:245	the influent	234:245	the influent (ambient water)	234:261	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	12	70	theme	novel	2326:2330	arg1	genus					2332:2336	a novel genus	2324:2336	a novel genus in the family Caulobacteraceae	2324:2367	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	14	71	theme	AB2021029T=KCTC	2489:2503	arg1	82788T					2505:2510	=CCTCC AB2021029T=KCTC 82788T	2482:2510	=CCTCC AB2021029T=KCTC 82788T	2482:2510	The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).
35201974	14	71	theme	AB2021029T=KCTC	2489:2503	arg1	SZ9T					2476:2479	SZ9T	2476:2479	SZ9T (=CCTCC AB2021029T=KCTC 82788T)	2476:2511	The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).
35201974	11	72	theme	acid	1925:1928	arg1	identity					1930:1937	average amino acid identity	1911:1937	average amino acid identity	1911:1937	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	6	73	theme	concentration	858:870	arg1	%					893:893	0-1.0 %	887:893	0-1.0 % (w/v)	887:899	The range of NaCl concentration for growth was 0-1.0 % (w/v), with an optimum of 0-0.5 % (w/v).
35201974	6	73	theme	concentration	858:870	arg1	range					844:848	The range	840:848	The range of NaCl concentration for growth	840:881	The range of NaCl concentration for growth was 0-1.0 % (w/v), with an optimum of 0-0.5 % (w/v).
35201974	12	74	theme	Pseudaquidulcibacter	2389:2408	arg1	nov.					2430:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	9	75	theme	major	1616:1620	arg1	phosphatidylcholine					1640:1658	phosphatidylcholine	1640:1658	phosphatidylcholine	1640:1658	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	9	75	theme	major	1616:1620	arg1	lipids					1628:1633	the major polar lipids	1612:1633	the major polar lipids	1612:1633	The sole respiratory quinone was ubiquinone-10, and the major polar lipids were phosphatidylcholine and two unidentified glycolipids.
35201974	1	76	theme	carbon	175:180	arg1	source					182:187	a carbon source	173:187	a carbon source	173:187	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	11	77	theme	DNA-DNA	1951:1957	arg1	hybridization					1959:1971	digital DNA-DNA hybridization	1943:1971	digital DNA-DNA hybridization	1943:1971	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	2	78	theme	microbial	209:217	arg1	communities					219:229	microbial communities	209:229	microbial communities	209:229	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	12	79	theme	phylogenetic	2185:2196	arg1	analyses					2198:2205	phenotypic, chemotaxonomic and phylogenetic analyses	2154:2205	phenotypic, chemotaxonomic and phylogenetic analyses	2154:2205	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	6	80	with	%	893:893	arg1	optimum					910:916	an optimum	907:916	an optimum of 0-0.5 % (w/v)	907:933	The range of NaCl concentration for growth was 0-1.0 % (w/v), with an optimum of 0-0.5 % (w/v).
35201974	10	81	from	%	1863:1863	arg1	size					1746:1749	size	1746:1749	size	1746:1749	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	8	82	theme	summed	1497:1502	arg1	feature					1504:1510	summed feature 8	1497:1512	summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c)	1497:1545	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	12	83	theme	genus	2332:2336	arg1	species					2313:2319	a novel species	2305:2319	a novel species	2305:2319	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	8	84	dep	C16 	1464:1467	arg1	C16 					1483:1486	C16 	1483:1486	C16 	1483:1486	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	84	dep	C16 	1464:1467	arg1	ω7c					1491:1493	ω7c	1491:1493	ω7c	1491:1493	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	84	dep	C16 	1464:1467	arg1	ω6c					1472:1474	ω6c	1472:1474	ω6c	1472:1474	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	3	85	from	0.5-1.0 mm	498:507	arg1	diameter					512:519	diameter	512:519	diameter	512:519	Colonies of strain SZ9T were small (approximately 0.5-1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex.
35201974	8	86	dep	acids	1377:1381	arg1	acids					1409:1413	the total fatty acids	1393:1413	the total fatty acids	1393:1413	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	86	dep	acids	1377:1381	arg1	%					1388:1388	>10 %	1384:1388	>10 % of the total fatty acids	1384:1413	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	4	87	theme	polar	641:645	arg1	flagellum					647:655	a single polar flagellum	632:655	a single polar flagellum	632:655	Cells were facultative anaerobic, motile by means of a single polar flagellum, rod-shaped, multiplied by binary fission, Gram-stain-negative, oxidase-positive and catalase-negative.
35201974	3	88	from	diameter	512:519	arg1	0.5-1.0 mm					498:507	0.5-1.0 mm	498:507	0.5-1.0 mm	498:507	Colonies of strain SZ9T were small (approximately 0.5-1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex.
35201974	10	89	theme	strain	1714:1719	arg1	SZ9T					1721:1724	strain SZ9T	1714:1724	strain SZ9T	1714:1724	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	1	90	theme	purification	122:133	arg1	facility					135:142	a water purification facility	114:142	a water purification facility with supplement of starch	114:168	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	12	91	theme	isolated	2212:2219	arg1	SZ9T					2228:2231	the isolated strain SZ9T	2208:2231	the isolated strain SZ9T	2208:2231	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	11	92	theme	nucleotide	1890:1899	arg1	identity					1901:1908	orthologous average nucleotide identity	1870:1908	orthologous average nucleotide identity	1870:1908	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	10	93	theme	41.4 mol	1855:1862	arg1	content					1843:1849	the genomic G+C content	1827:1849	the genomic G+C content	1827:1849	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	10	93	theme	41.4 mol	1855:1862	arg1	%					1863:1863	41.4 mol%	1855:1863	41.4 mol%	1855:1863	The whole genome of strain SZ9T was 2 842 140 bp in size, including 2769 protein-coding genes, 37 tRNA genes and two rRNA genes, and the genomic G+C content was 41.4 mol%.
35201974	7	94	theme	family	1046:1051	arg1	Caulobacteraceae					1053:1068	the family Caulobacteraceae	1042:1068	the family Caulobacteraceae of the class Alphaproteobacteria	1042:1101	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	11	95	theme	orthologous	1870:1880	arg1	identity					1901:1908	orthologous average nucleotide identity	1870:1908	orthologous average nucleotide identity	1870:1908	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	12	96	theme	novel	2307:2311	arg1	species					2313:2319	a novel species	2305:2319	a novel species	2305:2319	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	11	97	theme	amino	1919:1923	arg1	identity					1930:1937	average amino acid identity	1911:1937	average amino acid identity	1911:1937	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	1	98	theme	novel	59:63	arg1	member					65:70	a novel member	57:70	a novel member of family Caulobacteraceae	57:97	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	1	98	theme	novel	59:63	arg1	nov.					51:54	nov.	51:54	nov.	51:54	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	11	99	theme	%	2084:2084	arg1	values					1973:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	1866:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae	1866:2050	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	99	theme	%	2084:2084	arg1	%					2102:2102	64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %	2057:2102	%	2102:2102	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	5	100	dep	optimum	822:828	arg1	pH					831:832	pH 7.5	831:836	pH 7.5	831:836	Growth occurred at 10-40 °C (optimum, 28 °C) and pH 5.5-8.0 (optimum, pH 7.5).
35201974	2	101	theme	carbon	351:356	arg1	source					358:363	carbon source	351:363	carbon source	351:363	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	0	102	theme	saccharophilus	21:34	arg1	nov.					41:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	7	103	theme	Aquidulcibacter	1165:1179	arg1	TH1-2T					1197:1202	Aquidulcibacter paucihalophilus TH1-2T	1165:1202	Aquidulcibacter paucihalophilus TH1-2T (92.44%)	1165:1211	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	7	103	theme	Aquidulcibacter	1165:1179	arg1	%					1210:1210	92.44%	1205:1210	92.44%	1205:1210	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	8	104	theme	fatty	1371:1375	arg1	acids					1377:1381	The predominant fatty acids	1355:1381	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T	1355:1429	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	104	theme	fatty	1371:1375	arg1	feature					1442:1448	summed feature 3	1435:1450	summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c)	1435:1494	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	12	105	theme	other	2261:2265	arg1	genera					2267:2272	other genera	2261:2272	other genera	2261:2272	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	2	106	theme	starch	341:346	arg1	supplement					327:336	supplement	327:336	supplement	327:336	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	106	theme	starch	341:346	arg1	aeration					314:321	aeration	314:321	aeration	314:321	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	1	107	theme	Caulobacteraceae	82:97	arg1	member					65:70	a novel member	57:70	a novel member of family Caulobacteraceae	57:97	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	1	107	theme	Caulobacteraceae	82:97	arg1	nov.					51:54	nov.	51:54	nov.	51:54	nov., a novel member of family Caulobacteraceae, isolated from a water purification facility with supplement of starch as a carbon source.
35201974	11	108	theme	hybridization	1959:1971	arg1	values					1973:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	1866:1978	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae	1866:2050	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	11	108	theme	hybridization	1959:1971	arg1	%					2102:2102	64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %	2057:2102	%	2102:2102	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	2	109	theme	novel	368:372	arg1	strain					384:389	a novel bacterial strain	366:389	a novel bacterial strain	366:389	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	12	110	dep	sp	2436:2437	arg1	nov.					2430:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	the name Pseudaquidulcibacter saccharophilus gen. nov.	2380:2433	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	12	111	from	Caulobacteraceae	2352:2367	arg1	species					2313:2319	a novel species	2305:2319	a novel species	2305:2319	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	7	112	theme	gene	1135:1138	arg1	similarities					1149:1160	the highest 16S rRNA gene sequence similarities	1114:1160	the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%)	1114:1211	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	7	113	theme	rRNA	971:974	arg1	sequences					981:989	16S rRNA gene sequences	967:989	16S rRNA gene sequences	967:989	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	2	114	with	facility	300:307	arg1	supplement					327:336	supplement	327:336	supplement	327:336	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	114	with	facility	300:307	arg1	aeration					314:321	aeration	314:321	aeration	314:321	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	0	115	dep	sp	47:48	arg1	nov.					41:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov.	0:44	Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	7	116	theme	16S	1126:1128	arg1	similarities					1149:1160	the highest 16S rRNA gene sequence similarities	1114:1160	the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%)	1114:1211	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain SZ9T formed a lineage within the family Caulobacteraceae of the class Alphaproteobacteria and showed the highest 16S rRNA gene sequence similarities to Aquidulcibacter paucihalophilus TH1-2T (92.44%), followed by Vitreimonas flagellata SYSU XM001T (89.61 %), Asprobacter aquaticus DRW22-8T (89.49 %) and Hyphobacterium vulgare WM6T (89.49%).
35201974	2	117	theme	facility	300:307	arg1	water					256:260	ambient water	248:260	ambient water	248:260	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	117	theme	facility	300:307	arg1	effluent					267:274	effluent	267:274	effluent of a water purification facility with aeration and supplement of starch as carbon source	267:363	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	2	117	theme	facility	300:307	arg1	influent					238:245	the influent	234:245	the influent (ambient water)	234:261	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	12	118	from	genus	2332:2336	arg1	Caulobacteraceae					2352:2367	the family Caulobacteraceae	2341:2367	the family Caulobacteraceae	2341:2367	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	14	119	theme	type	2461:2464	arg1	SZ9T					2476:2479	SZ9T	2476:2479	SZ9T (=CCTCC AB2021029T=KCTC 82788T)	2476:2511	The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).
35201974	14	119	theme	type	2461:2464	arg1	strain					2466:2471	The type strain	2457:2471	The type strain	2457:2471	The type strain is SZ9T (=CCTCC AB2021029T=KCTC 82788T).
35201974	11	120	theme	other	2004:2008	arg1	genera					2010:2015	other genera	2004:2015	other genera	2004:2015	The orthologous average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between strain SZ9T and other genera within the family Caulobacteraceae were 64.50-66.62 %, 46.96-54.17 % and 27.70-31.70 %, respectively.
35201974	8	121	theme	fatty	1403:1407	arg1	acids					1409:1413	the total fatty acids	1393:1413	the total fatty acids	1393:1413	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	2	122	theme	water	281:285	arg1	facility					300:307	a water purification facility	279:307	a water purification facility with aeration and supplement of starch as carbon source	279:363	During a survey of microbial communities in the influent (ambient water) and effluent of a water purification facility with aeration and supplement of starch as carbon source, a novel bacterial strain, designated SZ9T, was isolated from the effluent sample.
35201974	12	123	theme	analyses	2198:2205	arg1	results					2143:2149	the results	2139:2149	the results of phenotypic, chemotaxonomic and phylogenetic analyses	2139:2205	Therefore, based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, the isolated strain SZ9T could be distinguished from other genera, suggesting that it represents a novel species of a novel genus in the family Caulobacteraceae, for which the name Pseudaquidulcibacter saccharophilus gen. nov., sp.
35201974	8	124	dep	C18 	1515:1518	arg1	C18 					1534:1537	C18 	1534:1537	C18 	1534:1537	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	124	dep	C18 	1515:1518	arg1	ω7c					1542:1544	ω7c	1542:1544	ω7c	1542:1544	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	8	124	dep	C18 	1515:1518	arg1	ω6c					1523:1525	ω6c	1523:1525	ω6c	1523:1525	The predominant fatty acids (>10 % of the total fatty acids) of strain SZ9T was summed feature 3 (comprising C16 : 1 ω6c and/or C16 : 1 ω7c), summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c) and C16 : 0.
35201974	3	125	theme	SZ9T	467:470	arg1	Colonies					448:455	Colonies	448:455	Colonies of strain SZ9T	448:470	Colonies of strain SZ9T were small (approximately 0.5-1.0 mm in diameter), creamy-white, circular, smooth, translucent and convex.
36326134	2	0	theme	production	562:571	arg1	capable					546:552	capable	546:552	capable	546:552	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	4	1	theme	decreased	1003:1011	arg1	scores					1021:1026	decreased the DAI scores	1003:1026	decreased the DAI scores	1003:1026	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	3	2	from	factors	699:705	arg1	contents					840:847	intestinal contents	829:847	intestinal contents	829:847	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	4	3	theme	DAI	1017:1019	arg1	scores					1021:1026	decreased the DAI scores	1003:1026	decreased the DAI scores	1003:1026	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	5	4	theme	Wnt/β-catenin	1383:1395	arg1	pathways					1397:1404	TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways	1355:1404	TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects	1355:1432	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	6	5	theme	intestinal	1494:1503	arg1	flora					1505:1509	the intestinal flora	1490:1509	the intestinal flora	1490:1509	In addition, three strains improved the composition of the intestinal flora and increased the production of SCFAs; notably, Propionibacterium freudenreichii B1 had the best effect.
36326134	1	6	theme	short-chain	260:270	arg1	metabolites					247:257	their major metabolites	235:257	their major metabolites	235:257	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	6	theme	short-chain	260:270	arg1	SCFAs					285:289	SCFAs	285:289	SCFAs	285:289	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	6	theme	short-chain	260:270	arg1	acids					278:282	short-chain fatty acids	260:282	short-chain fatty acids (SCFAs)	260:290	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	5	7	theme	TLRs/RHO	1355:1362	arg1	ROCK1					1372:1376	ROCK1	1372:1376	ROCK1	1372:1376	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	5	7	theme	TLRs/RHO	1355:1362	arg1	kinase					1364:1369	TLRs/RHO kinase	1355:1369	TLRs/RHO kinase (ROCK1)	1355:1377	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	2	8	theme	acetic	576:581	arg1	acid					583:586	acetic acid	576:586	acetic acid	576:586	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	3	9	from	content	818:824	arg1	contents					840:847	intestinal contents	829:847	intestinal contents	829:847	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	4	10	theme	decreased	1046:1054	arg1	levels					1079:1084	decreased proinflammatory factor levels	1046:1084	decreased proinflammatory factor levels	1046:1084	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	2	11	theme	C57BL/6J	405:412	arg1	mice					414:417	DSS-treated C57BL/6J mice	393:417	DSS-treated C57BL/6J mice	393:417	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	4	12	theme	factor	1072:1077	arg1	levels					1079:1084	decreased proinflammatory factor levels	1046:1084	decreased proinflammatory factor levels	1046:1084	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	1	13	theme	colitis	357:363	arg1	alleviation					331:341	the alleviation	327:341	the alleviation of ulcerative colitis (UC)	327:368	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	4	14	theme	increased	1111:1119	arg1	IL-10					1158:1162	IL-10	1158:1162	IL-10	1158:1162	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	14	theme	increased	1111:1119	arg1	production					1146:1155	increased anti-inflammatory factor production	1111:1155	increased anti-inflammatory factor production (IL-10)	1111:1163	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	3	15	from	effects	652:658	arg1	pathways					764:771	colitis-related signalling pathways	737:771	colitis-related signalling pathways	737:771	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	3	15	from	effects	652:658	arg1	factors					699:705	inflammatory factors	686:705	inflammatory factors	686:705	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	3	15	from	effects	652:658	arg1	composition					796:806	intestinal microbiome composition	774:806	intestinal microbiome composition	774:806	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	3	15	from	effects	652:658	arg1	content					818:824	SCFA content	813:824	SCFA content	813:824	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	3	15	from	effects	652:658	arg1	function					727:734	intestinal barrier function	708:734	intestinal barrier function	708:734	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	3	16	theme	intestinal	708:717	arg1	function					727:734	intestinal barrier function	708:734	intestinal barrier function	708:734	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	4	17	theme	experiment	869:878	arg1	results					854:860	The results	850:860	The results of the experiment	850:878	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	18	theme	factor	1139:1144	arg1	IL-10					1158:1162	IL-10	1158:1162	IL-10	1158:1162	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	18	theme	factor	1139:1144	arg1	production					1146:1155	increased anti-inflammatory factor production	1111:1155	increased anti-inflammatory factor production (IL-10)	1111:1163	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	3	19	theme	colitis-related	737:751	arg1	pathways					764:771	colitis-related signalling pathways	737:771	colitis-related signalling pathways	737:771	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	0	20	theme	ulcerative	74:83	arg1	colitis					85:91	DSS-induced ulcerative colitis	62:91	DSS-induced ulcerative colitis	62:91	Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology.
36326134	7	21	theme	further	1663:1669	arg1	application					1671:1681	the further application	1659:1681	the further application of probiotics in the treatment of UC in the future	1659:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	4	22	theme	reduced	953:959	arg1	loss					968:971	reduced weight loss	953:971	reduced weight loss	953:971	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	6	23	theme	best	1603:1606	arg1	effect					1608:1613	the best effect	1599:1613	the best effect	1599:1613	In addition, three strains improved the composition of the intestinal flora and increased the production of SCFAs; notably, Propionibacterium freudenreichii B1 had the best effect.
36326134	1	24	theme	bowel	189:193	arg1	IBD					204:206	IBD	204:206	IBD	204:206	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	24	theme	bowel	189:193	arg1	disease					195:201	inflammatory bowel disease	176:201	inflammatory bowel disease (IBD)	176:207	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	3	25	theme	intestinal	829:838	arg1	contents					840:847	intestinal contents	829:847	intestinal contents	829:847	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	2	26	theme	freudenreichii	486:499	arg1	B1					501:502	Propionibacterium freudenreichii B1	468:502	Propionibacterium freudenreichii B1	468:502	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	3	27	from	function	727:734	arg1	contents					840:847	intestinal contents	829:847	intestinal contents	829:847	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	3	28	theme	intestinal	774:783	arg1	composition					796:806	intestinal microbiome composition	774:806	intestinal microbiome composition	774:806	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	0	29	theme	intestinal	107:116	arg1	microecology					118:129	intestinal microecology	107:129	intestinal microecology	107:129	Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology.
36326134	7	30	theme	scientific	1638:1647	arg1	basis					1649:1653	a scientific basis	1636:1653	a scientific basis for the further application of probiotics in the treatment of UC in the future	1636:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	4	31	theme	tight	1179:1183	arg1	expression					1202:1211	enhanced tight junction protein expression	1170:1211	enhanced tight junction protein expression (ZO-1, occludin, and claudin-1)	1170:1243	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	5	32	theme	Bifidobacterium	1256:1270	arg1	H3-R2					1280:1284	Bifidobacterium bifidum H3-R2	1256:1284	Bifidobacterium bifidum H3-R2	1256:1284	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	6	33	theme	freudenreichii	1577:1590	arg1	B1					1592:1593	Propionibacterium freudenreichii B1	1559:1593	Propionibacterium freudenreichii B1	1559:1593	In addition, three strains improved the composition of the intestinal flora and increased the production of SCFAs; notably, Propionibacterium freudenreichii B1 had the best effect.
36326134	4	34	theme	splenic	988:994	arg1	index					996:1000	the splenic index	984:1000	decreased the splenic index	974:1000	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	5	35	theme	protective	1415:1424	arg1	effects					1426:1432	these protective effects	1409:1432	these protective effects	1409:1432	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	1	36	theme	fatty	272:276	arg1	metabolites					247:257	their major metabolites	235:257	their major metabolites	235:257	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	36	theme	fatty	272:276	arg1	SCFAs					285:289	SCFAs	285:289	SCFAs	285:289	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	36	theme	fatty	272:276	arg1	acids					278:282	short-chain fatty acids	260:282	short-chain fatty acids (SCFAs)	260:290	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	2	37	theme	present	378:384	arg1	study					386:390	the present study	374:390	the present study	374:390	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	2	38	theme	acid	616:619	arg1	production					562:571	high production	557:571	high production of acetic acid, propionic acid and butyric acid, respectively	557:633	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	0	39	theme	different	18:26	arg1	acids					46:50	different short-chain fatty acids	18:50	different short-chain fatty acids	18:50	Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology.
36326134	3	40	from	composition	796:806	arg1	contents					840:847	intestinal contents	829:847	intestinal contents	829:847	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	3	41	theme	SCFA	813:816	arg1	content					818:824	SCFA content	813:824	SCFA content	813:824	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	0	42	theme	fatty	40:44	arg1	acids					46:50	different short-chain fatty acids	18:50	different short-chain fatty acids	18:50	Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology.
36326134	4	43	theme	decreased	974:982	arg1	index					996:1000	the splenic index	984:1000	decreased the splenic index	974:1000	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	2	44	theme	Bifidobacterium	437:451	arg1	H3-R2					461:465	Bifidobacterium bifidum H3-R2	437:465	Bifidobacterium bifidum H3-R2	437:465	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	5	45	theme	crucial	1333:1339	arg1	roles					1341:1345	crucial roles	1333:1345	crucial roles	1333:1345	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	4	46	theme	MPO	1032:1034	arg1	activity					1036:1043	MPO activity	1032:1043	MPO activity	1032:1043	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	3	47	from	pathways	764:771	arg1	contents					840:847	intestinal contents	829:847	intestinal contents	829:847	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	4	48	theme	protein	1194:1200	arg1	expression					1202:1211	enhanced tight junction protein expression	1170:1211	enhanced tight junction protein expression (ZO-1, occludin, and claudin-1)	1170:1243	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	1	49	theme	ways	225:228	arg1	ways					225:228	ways	225:228	ways	225:228	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	49	theme	ways	225:228	arg1	variety					214:220	a variety	212:220	a variety of ways	212:228	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	2	50	dep	Bifidobacterium	437:451	arg1	bifidum					453:459	bifidum	453:459	bifidum	453:459	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	6	51	contain	had	1595:1597	arg2	effect					1608:1613	the best effect	1599:1613	the best effect	1599:1613	In addition, three strains improved the composition of the intestinal flora and increased the production of SCFAs; notably, Propionibacterium freudenreichii B1 had the best effect.
36326134	6	51	contain	had	1595:1597	arg1	B1					1592:1593	Propionibacterium freudenreichii B1	1559:1593	Propionibacterium freudenreichii B1	1559:1593	In addition, three strains improved the composition of the intestinal flora and increased the production of SCFAs; notably, Propionibacterium freudenreichii B1 had the best effect.
36326134	5	52	from	pathways	1397:1404	arg1	effects					1426:1432	these protective effects	1409:1432	these protective effects	1409:1432	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	1	53	theme	major	241:245	arg1	metabolites					247:257	their major metabolites	235:257	their major metabolites	235:257	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	53	theme	major	241:245	arg1	acids					278:282	short-chain fatty acids	260:282	short-chain fatty acids (SCFAs)	260:290	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	2	54	theme	high	557:560	arg1	production					562:571	high production	557:571	high production of acetic acid, propionic acid and butyric acid, respectively	557:633	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	3	55	theme	strains	675:681	arg1	effects					652:658	the effects	648:658	the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents	648:847	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	2	56	theme	acid	583:586	arg1	production					562:571	high production	557:571	high production of acetic acid, propionic acid and butyric acid, respectively	557:633	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	7	57	theme	UC	1717:1718	arg1	treatment					1704:1712	the treatment	1700:1712	the treatment of UC in the future	1700:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	7	58	from	application	1671:1681	arg1	treatment					1704:1712	the treatment	1700:1712	the treatment of UC in the future	1700:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	5	59	theme	kinase	1364:1369	arg1	pathways					1397:1404	TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways	1355:1404	TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects	1355:1432	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	7	60	from	probiotics	1686:1695	arg1	treatment					1704:1712	the treatment	1700:1712	the treatment of UC in the future	1700:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	4	61	theme	proinflammatory	1056:1070	arg1	levels					1079:1084	decreased proinflammatory factor levels	1046:1084	decreased proinflammatory factor levels	1046:1084	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	62	theme	enhanced	1170:1177	arg1	expression					1202:1211	enhanced tight junction protein expression	1170:1211	enhanced tight junction protein expression (ZO-1, occludin, and claudin-1)	1170:1243	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	2	63	theme	Clostridium	508:518	arg1	butyricum					520:528	Clostridium butyricum C1-6	508:533	Clostridium butyricum C1-6	508:533	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	3	64	theme	inflammatory	686:697	arg1	factors					699:705	inflammatory factors	686:705	inflammatory factors	686:705	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	1	65	theme	ulcerative	346:355	arg1	colitis					357:363	ulcerative colitis	346:363	ulcerative colitis (UC)	346:368	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	65	theme	ulcerative	346:355	arg1	UC					366:367	UC	366:367	UC	366:367	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	4	66	theme	anti-inflammatory	1121:1137	arg1	IL-10					1158:1162	IL-10	1158:1162	IL-10	1158:1162	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	66	theme	anti-inflammatory	1121:1137	arg1	production					1146:1155	increased anti-inflammatory factor production	1111:1155	increased anti-inflammatory factor production (IL-10)	1111:1163	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	6	67	theme	SCFAs	1543:1547	arg1	production					1529:1538	the production	1525:1538	the production of SCFAs	1525:1547	In addition, three strains improved the composition of the intestinal flora and increased the production of SCFAs; notably, Propionibacterium freudenreichii B1 had the best effect.
36326134	3	68	theme	barrier	719:725	arg1	function					727:734	intestinal barrier function	708:734	intestinal barrier function	708:734	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	4	69	dep	length	945:950	arg1	IL-8					1087:1090	IL-8	1087:1090	IL-8	1087:1090	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	69	dep	length	945:950	arg1	IL-1β					1093:1097	IL-1β	1093:1097	IL-1β	1093:1097	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	69	dep	length	945:950	arg1	scores					1021:1026	decreased the DAI scores	1003:1026	decreased the DAI scores	1003:1026	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	69	dep	length	945:950	arg1	levels					1079:1084	decreased proinflammatory factor levels	1046:1084	decreased proinflammatory factor levels	1046:1084	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	69	dep	length	945:950	arg1	index					996:1000	the splenic index	984:1000	decreased the splenic index	974:1000	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	69	dep	length	945:950	arg1	TNF-α					1103:1107	TNF-α	1103:1107	TNF-α	1103:1107	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	69	dep	length	945:950	arg1	loss					968:971	reduced weight loss	953:971	reduced weight loss	953:971	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	7	70	theme	probiotics	1686:1695	arg1	application					1671:1681	the further application	1659:1681	the further application of probiotics in the treatment of UC in the future	1659:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	4	71	dep	expression	1202:1211	arg1	occludin					1220:1227	occludin	1220:1227	occludin	1220:1227	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	71	dep	expression	1202:1211	arg1	claudin-1					1234:1242	claudin-1	1234:1242	claudin-1	1234:1242	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	71	dep	expression	1202:1211	arg1	ZO-1					1214:1217	ZO-1	1214:1217	ZO-1	1214:1217	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	4	72	theme	colon	939:943	arg1	length					945:950	the colon length	935:950	the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α)	935:1108	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	3	73	theme	signalling	753:762	arg1	pathways					764:771	colitis-related signalling pathways	737:771	colitis-related signalling pathways	737:771	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	6	74	theme	flora	1505:1509	arg1	composition					1475:1485	the composition	1471:1485	the composition of the intestinal flora	1471:1509	In addition, three strains improved the composition of the intestinal flora and increased the production of SCFAs; notably, Propionibacterium freudenreichii B1 had the best effect.
36326134	1	75	theme	inflammatory	176:187	arg1	IBD					204:206	IBD	204:206	IBD	204:206	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	1	75	theme	inflammatory	176:187	arg1	disease					195:201	inflammatory bowel disease	176:201	inflammatory bowel disease (IBD)	176:207	Probiotics have long been shown to modulate inflammatory bowel disease (IBD) in a variety of ways, and their major metabolites, short-chain fatty acids (SCFAs), have been shown to play a role in the alleviation of ulcerative colitis (UC).
36326134	2	76	theme	butyric	608:614	arg1	acid					616:619	butyric acid	608:619	butyric acid	608:619	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	2	77	theme	acid	599:602	arg1	production					562:571	high production	557:571	high production of acetic acid, propionic acid and butyric acid, respectively	557:633	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	4	78	theme	junction	1185:1192	arg1	expression					1202:1211	enhanced tight junction protein expression	1170:1211	enhanced tight junction protein expression (ZO-1, occludin, and claudin-1)	1170:1243	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	3	79	theme	microbiome	785:794	arg1	composition					796:806	intestinal microbiome composition	774:806	intestinal microbiome composition	774:806	We measured the effects of these three strains on inflammatory factors, intestinal barrier function, colitis-related signalling pathways, intestinal microbiome composition, and SCFA content in intestinal contents.
36326134	0	80	theme	short-chain	28:38	arg1	acids					46:50	different short-chain fatty acids	18:50	different short-chain fatty acids	18:50	Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology.
36326134	2	81	theme	propionic	589:597	arg1	acid					599:602	propionic acid	589:602	propionic acid	589:602	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	4	82	theme	weight	961:966	arg1	loss					968:971	reduced weight loss	953:971	reduced weight loss	953:971	The results of the experiment showed that all three strains differentially increased the colon length; reduced weight loss; decreased the splenic index; decreased the DAI scores and MPO activity; decreased proinflammatory factor levels (IL-8, IL-1β and TNF-α); increased anti-inflammatory factor production (IL-10); and enhanced tight junction protein expression (ZO-1, occludin, and claudin-1).
36326134	0	83	theme	DSS-induced	62:72	arg1	colitis					85:91	DSS-induced ulcerative colitis	62:91	DSS-induced ulcerative colitis	62:91	Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology.
36326134	7	84	from	treatment	1704:1712	arg1	future					1727:1732	future	1727:1732	future	1727:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	7	84	from	treatment	1704:1712	arg1	application					1671:1681	the further application	1659:1681	the further application of probiotics in the treatment of UC in the future	1659:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	2	85	theme	DSS-treated	393:403	arg1	mice					414:417	DSS-treated C57BL/6J mice	393:417	DSS-treated C57BL/6J mice	393:417	In the present study, DSS-treated C57BL/6J mice were gavaged with Bifidobacterium bifidum H3-R2, Propionibacterium freudenreichii B1 and Clostridium butyricum C1-6, which are capable of high production of acetic acid, propionic acid and butyric acid, respectively.
36326134	5	86	dep	Bifidobacterium	1256:1270	arg1	bifidum					1272:1278	bifidum	1272:1278	bifidum	1272:1278	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36326134	7	87	from	UC	1717:1718	arg1	future					1727:1732	future	1727:1732	future	1727:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	7	88	from	future	1727:1732	arg1	treatment					1704:1712	the treatment	1700:1712	the treatment of UC in the future	1700:1732	This study provides a scientific basis for the further application of probiotics in the treatment of UC in the future.
36326134	5	89	theme	freudenreichii	1308:1321	arg1	B1					1323:1324	Propionibacterium freudenreichii B1	1290:1324	Propionibacterium freudenreichii B1	1290:1324	Moreover, Bifidobacterium bifidum H3-R2 and Propionibacterium freudenreichii B1 played crucial roles through TLRs/RHO kinase (ROCK1) and Wnt/β-catenin pathways in these protective effects.
36706677	8	0	theme	saturated	1587:1595	arg1	acids					1641:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	3	1	theme	lipid	672:676	arg1	signaling					705:713	lipid raft-mediated inflammatory signaling	672:713	lipid raft-mediated inflammatory signaling	672:713	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	6	2	theme	decreased	1236:1244	arg1	expression					1251:1260	decreased PHB1 expression	1236:1260	decreased PHB1 expression	1236:1260	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	11	3	theme	lipid	2094:2098	arg1	rafts					2100:2104	lipid rafts	2094:2104	lipid rafts	2094:2104	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	8	4	theme	monounsaturated	1598:1612	arg1	acids					1641:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	10	5	theme	inflammatory	1909:1920	arg1	signaling					1922:1930	macrophage inflammatory signaling	1898:1930	macrophage inflammatory signaling	1898:1930	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	13	6	theme	necrosis	2542:2549	arg1	factor-alpha					2551:2562	tumor necrosis factor-alpha	2536:2562	tumor necrosis factor-alpha	2536:2562	To address this question, prohibitin knockdown macrophages were generated and responses to lipopolysaccharides and tumor necrosis factor-alpha, which act through lipid raft-dependent receptors, were analyzed.
36706677	11	7	theme	cell	2181:2184	arg1	functions					2186:2194	multiple other cell functions	2166:2194	multiple other cell functions	2166:2194	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	9	8	from	importance	1788:1797	arg1	formation					1813:1821	lipid raft formation	1802:1821	lipid raft formation	1802:1821	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	15	9	theme	macrophage	2909:2918	arg1	signaling					2933:2941	macrophage inflammatory signaling	2909:2941	macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation	2909:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	12	10	from	role	2338:2341	arg1	rafts					2378:2382	macrophage lipid rafts	2361:2382	macrophage lipid rafts	2361:2382	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	12	10	from	role	2338:2341	arg1	signaling					2399:2407	associated signaling	2388:2407	associated signaling	2388:2407	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	12	11	from	cells	2217:2221	arg1	response					2244:2251	the innate immune response	2226:2251	the innate immune response	2226:2251	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	5	12	theme	RAW	873:875	arg1	macrophages					883:893	RAW 264.7 macrophages	873:893	RAW 264.7 macrophages	873:893	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	7	13	theme	PHB2kd	1373:1378	arg1	macrophages					1380:1390	PHB2kd macrophages	1373:1390	PHB2kd macrophages	1373:1390	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	3	14	theme	PHBs	574:577	arg1	role					566:569	the specific role	553:569	the specific role of PHBs in the macrophage	553:595	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	1	15	theme	expressed	186:194	arg1	Prohibitins					141:151	Prohibitins	141:151	Prohibitins (PHB1 and PHB2)	141:167	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	1	15	theme	expressed	186:194	arg1	proteins					196:203	ubiquitously expressed proteins	173:203	ubiquitously expressed proteins which play critical roles in multiple biological processes	173:262	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	6	16	theme	factor-kappa-B	1289:1302	arg1	activation					1312:1321	attenuated nuclear factor-kappa-B (NF-κB) activation	1270:1321	attenuated nuclear factor-kappa-B (NF-κB) activation	1270:1321	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	14	17	theme	Prohibitin	2630:2639	arg1	macrophages					2651:2661	Prohibitin knockdown macrophages	2630:2661	Prohibitin knockdown macrophages	2630:2661	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	9	18	theme	decreased	1672:1680	arg1	formation					1693:1701	decreased lipid raft formation	1672:1701	decreased lipid raft formation	1672:1701	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	14	19	theme	fatty	2834:2838	arg1	remodeling					2845:2854	altered fatty acid remodeling	2826:2854	altered fatty acid remodeling	2826:2854	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	6	20	theme	attenuated	1270:1279	arg1	activation					1312:1321	attenuated nuclear factor-kappa-B (NF-κB) activation	1270:1321	attenuated nuclear factor-kappa-B (NF-κB) activation	1270:1321	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	1	21	theme	critical	216:223	arg1	roles					225:229	critical roles	216:229	critical roles	216:229	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	9	22	theme	raft	1688:1691	arg1	formation					1693:1701	decreased lipid raft formation	1672:1701	decreased lipid raft formation	1672:1701	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	15	23	theme	composition	2976:2986	arg1	regulation					2951:2960	regulation	2951:2960	regulation of fatty acid composition and lipid raft formation	2951:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	10	24	theme	critical	1861:1868	arg1	role					1870:1873	a critical role	1859:1873	a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure	1859:1997	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	10	25	theme	acid	1957:1960	arg1	composition					1962:1972	fatty acid composition	1951:1972	fatty acid composition	1951:1972	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	5	26	theme	tumor	1024:1028	arg1	TNF-α					1053:1057	TNF-α	1053:1057	TNF-α	1053:1057	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	5	26	theme	tumor	1024:1028	arg1	factor-alpha					1039:1050	tumor necrosis factor-alpha	1024:1050	tumor necrosis factor-alpha (TNF-α)	1024:1058	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	15	27	from	role	2885:2888	arg1	signaling					2933:2941	macrophage inflammatory signaling	2909:2941	macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation	2909:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	11	28	theme	cellular	2061:2068	arg1	compartments					2070:2081	phospholipid-rich cellular compartments	2043:2081	phospholipid-rich cellular compartments	2043:2081	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	12	29	theme	lipid	2282:2286	arg1	rafts					2288:2292	membrane lipid rafts	2273:2292	membrane lipid rafts	2273:2292	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	15	30	theme	fatty	2965:2969	arg1	composition					2976:2986	fatty acid composition	2965:2986	fatty acid composition	2965:2986	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	3	31	from	role	566:569	arg1	macrophage					586:595	the macrophage	582:595	the macrophage	582:595	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	5	32	theme	necrosis	1030:1037	arg1	TNF-α					1053:1057	TNF-α	1053:1057	TNF-α	1053:1057	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	5	32	theme	necrosis	1030:1037	arg1	factor-alpha					1039:1050	tumor necrosis factor-alpha	1024:1050	tumor necrosis factor-alpha (TNF-α)	1024:1058	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	9	33	theme	plasma	1716:1721	arg1	packing					1742:1748	modified plasma membrane molecular packing	1707:1748	modified plasma membrane molecular packing	1707:1748	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	10	34	theme	raft	1984:1987	arg1	structure					1989:1997	lipid raft structure	1978:1997	lipid raft structure	1978:1997	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	5	35	theme	receptor	1097:1104	arg1	signaling					1106:1114	lipid raft-dependent receptor signaling	1076:1114	lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively)	1076:1150	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	2	36	theme	B	496:496	arg1	lymphocyte					498:507	B lymphocyte	496:507	B lymphocyte	496:507	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	0	37	theme	membrane	75:82	arg1	packing					84:90	plasma membrane packing	68:90	plasma membrane packing	68:90	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	15	38	theme	acid	2971:2974	arg1	composition					2976:2986	fatty acid composition	2965:2986	fatty acid composition	2965:2986	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	7	39	theme	cell	1411:1414	arg1	surface					1416:1422	cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1	1411:1479	cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1	1411:1479	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	12	40	theme	innate	2230:2235	arg1	response					2244:2251	the innate immune response	2226:2251	the innate immune response	2226:2251	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	4	41	theme	inflammatory	784:795	arg1	signaling					797:805	macrophage inflammatory signaling	773:805	macrophage inflammatory signaling	773:805	Therefore, we hypothesize that the PHB complex regulates macrophage inflammatory signaling through the formation of lipid rafts.
36706677	0	42	theme	lipid	97:101	arg1	signaling					130:138	lipid raft-mediated inflammatory signaling	97:138	lipid raft-mediated inflammatory signaling	97:138	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	5	43	theme	lipid	1076:1080	arg1	signaling					1106:1114	lipid raft-dependent receptor signaling	1076:1114	lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively)	1076:1150	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	14	44	theme	lipid	2780:2784	arg1	assembly					2791:2798	lipid raft assembly	2780:2798	lipid raft assembly	2780:2798	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	9	45	theme	PHB	1774:1776	arg1	complex					1778:1784	PHB complex	1774:1784	the PHB complex's importance in lipid raft formation	1770:1821	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	5	46	dep	signaling	1106:1114	arg1	TNFR1					1131:1135	TNFR1	1131:1135	TNFR1	1131:1135	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	5	46	dep	signaling	1106:1114	arg1	CD14/TLR4					1117:1125	CD14/TLR4	1117:1125	CD14/TLR4	1117:1125	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	0	47	theme	plasma	68:73	arg1	packing					84:90	plasma membrane packing	68:90	plasma membrane packing	68:90	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	13	48	theme	lipid	2583:2587	arg1	receptors					2604:2612	lipid raft-dependent receptors	2583:2612	lipid raft-dependent receptors	2583:2612	To address this question, prohibitin knockdown macrophages were generated and responses to lipopolysaccharides and tumor necrosis factor-alpha, which act through lipid raft-dependent receptors, were analyzed.
36706677	2	49	theme	transport	445:453	arg1	PHB1					412:415	PHB1	412:415	PHB1	412:415	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	2	49	theme	transport	445:453	arg1	mediator					422:429	a mediator	420:429	a mediator of fatty acid transport	420:453	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	2	49	theme	transport	445:453	arg1	scaffold					477:484	a membrane scaffold	466:484	a membrane scaffold mediating B lymphocyte and mast cell signal transduction	466:541	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	4	50	theme	PHB	751:753	arg1	complex					755:761	the PHB complex	747:761	the PHB complex	747:761	Therefore, we hypothesize that the PHB complex regulates macrophage inflammatory signaling through the formation of lipid rafts.
36706677	2	51	theme	fatty	434:438	arg1	transport					445:453	fatty acid transport	434:453	fatty acid transport	434:453	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	1	52	theme	ring-like	287:295	arg1	complex					301:307	the ring-like PHB complex	283:307	the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts	283:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	11	53	theme	other	2175:2179	arg1	functions					2186:2194	multiple other cell functions	2166:2194	multiple other cell functions	2166:2194	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	8	54	theme	Post-LPS	1511:1518	arg1	macrophages					1528:1538	Post-LPS, PHB2kd macrophages	1511:1538	Post-LPS, PHB2kd macrophages	1511:1538	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	4	55	theme	lipid	832:836	arg1	rafts					838:842	lipid rafts	832:842	lipid rafts	832:842	Therefore, we hypothesize that the PHB complex regulates macrophage inflammatory signaling through the formation of lipid rafts.
36706677	0	56	theme	macrophage	33:42	arg1	composition					55:65	macrophage fatty acid composition	33:65	macrophage fatty acid composition	33:65	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	7	57	dep	surface	1416:1422	arg1	TLR4					1437:1440	TLR4	1437:1440	TLR4	1437:1440	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	7	57	dep	surface	1416:1422	arg1	CD14					1431:1434	CD14	1431:1434	CD14	1431:1434	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	7	57	dep	surface	1416:1422	arg1	TNFR1					1424:1428	TNFR1	1424:1428	TNFR1	1424:1428	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	7	57	dep	surface	1416:1422	arg1	GM1					1477:1479	lipid raft marker ganglioside GM1	1447:1479	lipid raft marker ganglioside GM1	1447:1479	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	2	58	theme	membrane	468:475	arg1	PHB1					412:415	PHB1	412:415	PHB1	412:415	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	2	58	theme	membrane	468:475	arg1	scaffold					477:484	a membrane scaffold	466:484	a membrane scaffold mediating B lymphocyte and mast cell signal transduction	466:541	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	14	59	theme	factor	2740:2745	arg1	activation					2747:2756	transcription factor activation	2726:2756	transcription factor activation	2726:2756	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	9	60	theme	raft	1808:1811	arg1	formation					1813:1821	lipid raft formation	1802:1821	lipid raft formation	1802:1821	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	0	61	theme	acid	50:53	arg1	composition					55:65	macrophage fatty acid composition	33:65	macrophage fatty acid composition	33:65	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	1	62	theme	cellular	336:343	arg1	compartments					345:356	phospholipid-rich cellular compartments	318:356	phospholipid-rich cellular compartments including lipid rafts	318:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	1	62	theme	cellular	336:343	arg1	rafts					374:378	lipid rafts	368:378	lipid rafts	368:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	7	63	theme	marker	1458:1463	arg1	GM1					1477:1479	lipid raft marker ganglioside GM1	1447:1479	lipid raft marker ganglioside GM1	1447:1479	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	12	64	theme	associated	2388:2397	arg1	signaling					2399:2407	associated signaling	2388:2407	associated signaling	2388:2407	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	2	65	dep	lymphocyte	498:507	arg1	transduction					530:541	signal transduction	523:541	signal transduction	523:541	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	7	66	theme	lipid	1447:1451	arg1	GM1					1477:1479	lipid raft marker ganglioside GM1	1447:1479	lipid raft marker ganglioside GM1	1447:1479	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	14	67	theme	chemokine	2704:2712	arg1	production					2714:2723	cytokine and chemokine production	2691:2723	cytokine and chemokine production	2691:2723	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	12	68	theme	prohibitins	2346:2356	arg1	role					2338:2341	the role	2334:2341	the role of prohibitins in macrophage lipid rafts and associated signaling	2334:2407	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	12	68	theme	prohibitins	2346:2356	arg1	unknown					2412:2418	unknown	2412:2418	unknown	2412:2418	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	3	69	theme	acid	656:659	arg1	uptake					661:666	fatty acid uptake	650:666	fatty acid uptake	650:666	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	8	70	theme	polyunsaturated	1619:1633	arg1	acids					1641:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	1	71	theme	multiple	234:241	arg1	processes					254:262	multiple biological processes	234:262	multiple biological processes	234:262	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	14	72	theme	cytokine	2691:2698	arg1	production					2714:2723	cytokine and chemokine production	2691:2723	cytokine and chemokine production	2691:2723	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	12	73	theme	macrophage	2361:2370	arg1	rafts					2378:2382	macrophage lipid rafts	2361:2382	macrophage lipid rafts	2361:2382	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	9	74	theme	molecular	1732:1740	arg1	packing					1742:1748	modified plasma membrane molecular packing	1707:1748	modified plasma membrane molecular packing	1707:1748	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	6	75	theme	PHB1	1246:1249	arg1	expression					1251:1260	decreased PHB1 expression	1236:1260	decreased PHB1 expression	1236:1260	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	8	76	theme	acids	1641:1645	arg1	concentration					1561:1573	the concentration	1557:1573	the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids	1557:1645	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	8	77	theme	cellular	1578:1585	arg1	acids					1641:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	3	78	theme	raft-mediated	678:690	arg1	signaling					705:713	lipid raft-mediated inflammatory signaling	672:713	lipid raft-mediated inflammatory signaling	672:713	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	13	79	theme	tumor	2536:2540	arg1	factor-alpha					2551:2562	tumor necrosis factor-alpha	2536:2562	tumor necrosis factor-alpha	2536:2562	To address this question, prohibitin knockdown macrophages were generated and responses to lipopolysaccharides and tumor necrosis factor-alpha, which act through lipid raft-dependent receptors, were analyzed.
36706677	6	80	theme	PHB1	1153:1156	arg1	knockdown					1158:1166	PHB1 knockdown	1153:1166	PHB1 knockdown	1153:1166	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	3	81	from	role	642:645	arg1	signaling					705:713	lipid raft-mediated inflammatory signaling	672:713	lipid raft-mediated inflammatory signaling	672:713	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	3	81	from	role	642:645	arg1	uptake					661:666	fatty acid uptake	650:666	fatty acid uptake	650:666	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	10	82	theme	macrophage	1898:1907	arg1	signaling					1922:1930	macrophage inflammatory signaling	1898:1930	macrophage inflammatory signaling	1898:1930	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	15	83	theme	novel	2879:2883	arg1	role					2885:2888	a novel role	2877:2888	a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation	2877:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	15	84	theme	inflammatory	2920:2931	arg1	signaling					2933:2941	macrophage inflammatory signaling	2909:2941	macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation	2909:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	6	85	theme	subsequent	1327:1336	arg1	cytokine					1338:1345	subsequent cytokine and chemokine production	1327:1370	cytokine	1338:1345	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	11	86	theme	important	2117:2125	arg1	roles					2127:2131	important roles	2117:2131	important roles	2117:2131	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	13	87	theme	prohibitin	2447:2456	arg1	macrophages					2468:2478	prohibitin knockdown macrophages	2447:2478	prohibitin knockdown macrophages	2447:2478	To address this question, prohibitin knockdown macrophages were generated and responses to lipopolysaccharides and tumor necrosis factor-alpha, which act through lipid raft-dependent receptors, were analyzed.
36706677	3	88	theme	specific	557:564	arg1	role					566:569	the specific role	553:569	the specific role of PHBs in the macrophage	553:595	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	14	89	theme	knockdown	2641:2649	arg1	macrophages					2651:2661	Prohibitin knockdown macrophages	2630:2661	Prohibitin knockdown macrophages	2630:2661	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	11	90	theme	phospholipid-rich	2043:2059	arg1	compartments					2070:2081	phospholipid-rich cellular compartments	2043:2081	phospholipid-rich cellular compartments	2043:2081	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	12	91	theme	signal	2309:2314	arg1	transduction					2316:2327	signal transduction	2309:2327	signal transduction	2309:2327	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	14	92	theme	acid	2840:2843	arg1	remodeling					2845:2854	altered fatty acid remodeling	2826:2854	altered fatty acid remodeling	2826:2854	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	6	93	theme	nuclear	1281:1287	arg1	NF-κB					1305:1309	NF-κB	1305:1309	NF-κB	1305:1309	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	6	93	theme	nuclear	1281:1287	arg1	factor-kappa-B					1289:1302	nuclear factor-kappa-B	1281:1302	attenuated nuclear factor-kappa-B (NF-κB) activation	1270:1321	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	11	94	located	found	2034:2038	arg1	compartments					2070:2081	phospholipid-rich cellular compartments	2043:2081	phospholipid-rich cellular compartments	2043:2081	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	11	94	located	found	2034:2038	arg2	SUMMARY					2000:2006	SUMMARY	2000:2006	SUMMARY: Prohibitins	2000:2019	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	11	94	located	found	2034:2038	arg2	rafts					2100:2104	lipid rafts	2094:2104	lipid rafts	2094:2104	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	11	94	located	found	2034:2038	arg2	proteins					2025:2032	proteins	2025:2032	proteins	2025:2032	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	9	95	theme	lipid	1682:1686	arg1	formation					1693:1701	decreased lipid raft formation	1672:1701	decreased lipid raft formation	1672:1701	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	14	96	theme	altered	2826:2832	arg1	remodeling					2845:2854	altered fatty acid remodeling	2826:2854	altered fatty acid remodeling	2826:2854	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	10	97	theme	composition	1962:1972	arg1	maintenance					1936:1946	maintenance	1936:1946	maintenance of fatty acid composition and lipid raft structure	1936:1997	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	12	98	theme	rafts	2288:2292	arg1	presence					2261:2268	the presence	2257:2268	the presence of membrane lipid rafts	2257:2292	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	12	98	theme	rafts	2288:2292	arg1	integral					2297:2304	integral	2297:2304	integral	2297:2304	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	6	99	theme	PHB2	1188:1191	arg1	knockdown					1193:1201	PHB2 knockdown	1188:1201	PHB2 knockdown (PHB2kd)	1188:1210	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	6	99	theme	PHB2	1188:1191	arg1	PHB2kd					1204:1209	PHB2kd	1204:1209	PHB2kd	1204:1209	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	10	100	theme	fatty	1951:1955	arg1	composition					1962:1972	fatty acid composition	1951:1972	fatty acid composition	1951:1972	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	8	101	dep	Post-LPS	1511:1518	arg1	PHB2kd					1521:1526	PHB2kd	1521:1526	PHB2kd	1521:1526	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	9	102	theme	modified	1707:1714	arg1	packing					1742:1748	modified plasma membrane molecular packing	1707:1748	modified plasma membrane molecular packing	1707:1748	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	14	103	theme	membrane	2804:2811	arg1	packing					2813:2819	membrane packing	2804:2819	membrane packing	2804:2819	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	10	104	theme	structure	1989:1997	arg1	maintenance					1936:1946	maintenance	1936:1946	maintenance of fatty acid composition and lipid raft structure	1936:1997	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	6	105	theme	chemokine	1351:1359	arg1	production					1361:1370	subsequent cytokine and chemokine production	1327:1370	production	1361:1370	PHB1 knockdown was lethal, whereas PHB2 knockdown (PHB2kd), which also resulted in decreased PHB1 expression, led to attenuated nuclear factor-kappa-B (NF-κB) activation and subsequent cytokine and chemokine production.
36706677	11	106	dep	SUMMARY	2000:2006	arg1	Prohibitins					2009:2019	Prohibitins	2009:2019	Prohibitins	2009:2019	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	9	107	theme	membrane	1723:1730	arg1	packing					1742:1748	modified plasma membrane molecular packing	1707:1748	modified plasma membrane molecular packing	1707:1748	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	10	108	theme	lipid	1978:1982	arg1	structure					1989:1997	lipid raft structure	1978:1997	lipid raft structure	1978:1997	Taken together, these data suggest a critical role for PHBs in regulating macrophage inflammatory signaling via maintenance of fatty acid composition and lipid raft structure.
36706677	0	109	theme	inflammatory	117:128	arg1	signaling					130:138	lipid raft-mediated inflammatory signaling	97:138	lipid raft-mediated inflammatory signaling	97:138	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	12	110	theme	membrane	2273:2280	arg1	rafts					2288:2292	membrane lipid rafts	2273:2292	membrane lipid rafts	2273:2292	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	1	111	theme	lipid	368:372	arg1	rafts					374:378	lipid rafts	368:378	lipid rafts	368:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	2	112	theme	signal	523:528	arg1	transduction					530:541	signal transduction	523:541	signal transduction	523:541	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	12	113	theme	immune	2237:2242	arg1	response					2244:2251	the innate immune response	2226:2251	the innate immune response	2226:2251	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	12	114	theme	key	2213:2215	arg1	Macrophages					2197:2207	Macrophages	2197:2207	Macrophages	2197:2207	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	12	114	theme	key	2213:2215	arg1	cells					2217:2221	key cells	2213:2221	key cells in the innate immune response	2213:2251	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	1	115	dep	Prohibitins	141:151	arg1	PHB1					154:157	PHB1	154:157	PHB1	154:157	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	1	115	dep	Prohibitins	141:151	arg1	Prohibitins					141:151	Prohibitins	141:151	Prohibitins (PHB1 and PHB2)	141:167	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	1	115	dep	Prohibitins	141:151	arg1	PHB2					163:166	PHB2	163:166	PHB2	163:166	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	0	116	theme	raft-mediated	103:115	arg1	signaling					130:138	lipid raft-mediated inflammatory signaling	97:138	lipid raft-mediated inflammatory signaling	97:138	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	14	117	theme	raft	2786:2789	arg1	assembly					2791:2798	lipid raft assembly	2780:2798	lipid raft assembly	2780:2798	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	2	118	theme	mast	513:516	arg1	cell					518:521	mast cell	513:521	mast cell	513:521	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	13	119	theme	knockdown	2458:2466	arg1	macrophages					2468:2478	prohibitin knockdown macrophages	2447:2478	prohibitin knockdown macrophages	2447:2478	To address this question, prohibitin knockdown macrophages were generated and responses to lipopolysaccharides and tumor necrosis factor-alpha, which act through lipid raft-dependent receptors, were analyzed.
36706677	4	120	theme	macrophage	773:782	arg1	signaling					797:805	macrophage inflammatory signaling	773:805	macrophage inflammatory signaling	773:805	Therefore, we hypothesize that the PHB complex regulates macrophage inflammatory signaling through the formation of lipid rafts.
36706677	0	121	theme	prohibitin	4:13	arg1	complex					15:21	The prohibitin complex	0:21	The prohibitin complex	0:21	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	5	122	theme	raft-dependent	1082:1095	arg1	signaling					1106:1114	lipid raft-dependent receptor signaling	1076:1114	lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively)	1076:1150	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	1	123	located	found	309:313	arg1	compartments					345:356	phospholipid-rich cellular compartments	318:356	phospholipid-rich cellular compartments including lipid rafts	318:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	1	123	located	found	309:313	arg1	rafts					374:378	lipid rafts	368:378	lipid rafts	368:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	1	123	located	found	309:313	arg2	complex					301:307	the ring-like PHB complex	283:307	the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts	283:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	13	124	theme	raft-dependent	2589:2602	arg1	receptors					2604:2612	lipid raft-dependent receptors	2583:2612	lipid raft-dependent receptors	2583:2612	To address this question, prohibitin knockdown macrophages were generated and responses to lipopolysaccharides and tumor necrosis factor-alpha, which act through lipid raft-dependent receptors, were analyzed.
36706677	5	125	theme	scrambled	945:953	arg1	Scr					964:966	Scr	964:966	Scr	964:966	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	5	125	theme	scrambled	945:953	arg1	control					955:961	scrambled control	945:961	scrambled control (Scr)	945:967	To evaluate our hypothesis, RAW 264.7 macrophages were transduced with shRNA against PHB1, PHB2, or scrambled control (Scr), and then stimulated with lipopolysaccharides (LPS) or tumor necrosis factor-alpha (TNF-α), which activate lipid raft-dependent receptor signaling (CD14/TLR4 and TNFR1, respectively).
36706677	15	126	theme	lipid	2992:2996	arg1	formation					3003:3011	lipid raft formation	2992:3011	lipid raft formation	2992:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	14	127	theme	receptor	2759:2766	arg1	expression					2768:2777	receptor expression	2759:2777	receptor expression	2759:2777	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	2	128	theme	acid	440:443	arg1	transport					445:453	fatty acid transport	434:453	fatty acid transport	434:453	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	0	129	theme	fatty	44:48	arg1	composition					55:65	macrophage fatty acid composition	33:65	macrophage fatty acid composition	33:65	The prohibitin complex regulates macrophage fatty acid composition, plasma membrane packing, and lipid raft-mediated inflammatory signaling.
36706677	1	130	theme	PHB	297:299	arg1	complex					301:307	the ring-like PHB complex	283:307	the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts	283:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	15	131	theme	formation	3003:3011	arg1	regulation					2951:2960	regulation	2951:2960	regulation of fatty acid composition and lipid raft formation	2951:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	9	132	theme	lipid	1802:1806	arg1	formation					1813:1821	lipid raft formation	1802:1821	lipid raft formation	1802:1821	This was accompanied by decreased lipid raft formation and modified plasma membrane molecular packing, further supporting the PHB complex's importance in lipid raft formation.
36706677	14	133	theme	transcription	2726:2738	arg1	activation					2747:2756	transcription factor activation	2726:2756	transcription factor activation	2726:2756	Prohibitin knockdown macrophages had significantly decreased cytokine and chemokine production, transcription factor activation, receptor expression, lipid raft assembly and membrane packing, and altered fatty acid remodeling.
36706677	11	134	theme	multiple	2166:2173	arg1	functions					2186:2194	multiple other cell functions	2166:2194	multiple other cell functions	2166:2194	SUMMARY: Prohibitins are proteins found in phospholipid-rich cellular compartments, including lipid rafts, that play important roles in signaling, transcription, and multiple other cell functions.
36706677	4	135	theme	rafts	838:842	arg1	formation					819:827	the formation	815:827	the formation of lipid rafts	815:842	Therefore, we hypothesize that the PHB complex regulates macrophage inflammatory signaling through the formation of lipid rafts.
36706677	1	136	theme	phospholipid-rich	318:334	arg1	compartments					345:356	phospholipid-rich cellular compartments	318:356	phospholipid-rich cellular compartments including lipid rafts	318:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	1	136	theme	phospholipid-rich	318:334	arg1	rafts					374:378	lipid rafts	368:378	lipid rafts	368:378	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36706677	7	137	theme	ganglioside	1465:1475	arg1	GM1					1477:1479	lipid raft marker ganglioside GM1	1447:1479	lipid raft marker ganglioside GM1	1447:1479	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	15	138	theme	raft	2998:3001	arg1	formation					3003:3011	lipid raft formation	2992:3011	lipid raft formation	2992:3011	These data indicate a novel role for prohibitins in macrophage inflammatory signaling through regulation of fatty acid composition and lipid raft formation.
36706677	3	139	theme	fatty	650:654	arg1	uptake					661:666	fatty acid uptake	650:666	fatty acid uptake	650:666	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	8	140	theme	fatty	1635:1639	arg1	acids					1641:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	cellular saturated, monounsaturated, and polyunsaturated fatty acids	1578:1645	Post-LPS, PHB2kd macrophages did not increase the concentration of cellular saturated, monounsaturated, and polyunsaturated fatty acids.
36706677	7	141	theme	raft	1453:1456	arg1	GM1					1477:1479	lipid raft marker ganglioside GM1	1447:1479	lipid raft marker ganglioside GM1	1447:1479	PHB2kd macrophages also had decreased cell surface TNFR1, CD14, TLR4, and lipid raft marker ganglioside GM1 at baseline and post-stimuli.
36706677	12	142	theme	lipid	2372:2376	arg1	rafts					2378:2382	macrophage lipid rafts	2361:2382	macrophage lipid rafts	2361:2382	Macrophages are key cells in the innate immune response and the presence of membrane lipid rafts is integral to signal transduction, but the role of prohibitins in macrophage lipid rafts and associated signaling is unknown.
36706677	3	143	theme	inflammatory	692:703	arg1	signaling					705:713	lipid raft-mediated inflammatory signaling	672:713	lipid raft-mediated inflammatory signaling	672:713	However, the specific role of PHBs in the macrophage have not been characterized, including their role in fatty acid uptake and lipid raft-mediated inflammatory signaling.
36706677	2	144	theme	Recent	381:386	arg1	studies					388:394	Recent studies	381:394	Recent studies	381:394	Recent studies have implicated PHB1 as a mediator of fatty acid transport as well as a membrane scaffold mediating B lymphocyte and mast cell signal transduction.
36706677	1	145	theme	biological	243:252	arg1	processes					254:262	multiple biological processes	234:262	multiple biological processes	234:262	Prohibitins (PHB1 and PHB2) are ubiquitously expressed proteins which play critical roles in multiple biological processes, and together form the ring-like PHB complex found in phospholipid-rich cellular compartments including lipid rafts.
36770726	5	0	theme	protective	651:660	arg1	effects					662:668	the protective effects	647:668	the protective effects	647:668	This study was designed to explore the protective effects and mechanisms of CEP in treating colitis through in vitro and in vivo experiments.
36770726	8	1	theme	gut	1126:1128	arg1	microbiota					1130:1139	gut microbiota	1126:1139	gut microbiota	1126:1139	After treatment with antibiotics (Abx), the expression of ACOD1 changed with the composition of gut microbiota.
36770726	10	2	theme	integrative	1314:1324	arg1	iHMP					1352:1355	iHMP	1352:1355	iHMP	1352:1355	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	10	2	theme	integrative	1314:1324	arg1	Project					1343:1349	the integrative Human Microbiome Project	1310:1349	the integrative Human Microbiome Project (iHMP)	1310:1356	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	6	3	theme	pro-inflammatory	824:839	arg1	cytokines					841:849	pro-inflammatory cytokines	824:849	pro-inflammatory cytokines	824:849	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	4	4	theme	ACOD1	575:579	arg1	expression					581:590	ACOD1 expression	575:590	ACOD1 expression	575:590	However, whether CEP can combat UC by affecting ACOD1 expression remains unanswered.
36770726	7	5	theme	ACOD1	996:1000	arg1	expression					1002:1011	ACOD1 expression	996:1011	ACOD1 expression	996:1011	Additionally, in the mouse model of DSS-induced colitis, CEP decreased macrophage infiltration and ACOD1 expression in colon tissue.
36770726	0	6	theme	Macrophage	112:121	arg1	Infiltration					123:134	Macrophage Infiltration	112:134	Macrophage Infiltration	112:134	Cepharanthine Alleviates DSS-Induced Ulcerative Colitis via Regulating Aconitate Decarboxylase 1 Expression and Macrophage Infiltration.
36770726	9	7	theme	Correlation	1142:1152	arg1	analysis					1154:1161	Correlation analysis	1142:1161	Correlation analysis	1142:1161	Correlation analysis also revealed that Family-XIII-AD3011-group and Rumini-clostridium-6 were positively correlated with ACOD1 expression level.
36770726	7	8	theme	macrophage	968:977	arg1	infiltration					979:990	macrophage infiltration	968:990	macrophage infiltration	968:990	Additionally, in the mouse model of DSS-induced colitis, CEP decreased macrophage infiltration and ACOD1 expression in colon tissue.
36770726	6	9	from	expression	861:870	arg1	macrophages					884:894	RAW264.7 macrophages	875:894	RAW264.7 macrophages	875:894	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	6	10	theme	LPS-induced	799:809	arg1	secretion					811:819	LPS-induced secretion	799:819	LPS-induced secretion of pro-inflammatory cytokines	799:849	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	10	11	theme	colon	1404:1408	arg1	tissue					1410:1415	the colon tissue	1400:1415	the colon tissue of UC patients	1400:1430	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	6	12	from	secretion	811:819	arg1	macrophages					884:894	RAW264.7 macrophages	875:894	RAW264.7 macrophages	875:894	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	11	13	theme	ACOD1	1619:1623	arg1	expression					1605:1614	the expression	1601:1614	the expression of ACOD1	1601:1623	Collectively, CEP can counter UC by modulating gut microbiota and inhibiting the expression of ACOD1.
36770726	10	14	theme	Project	1343:1349	arg1	data					1302:1305	data	1302:1305	data of the integrative Human Microbiome Project (iHMP)	1302:1356	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	2	15	theme	inflammatory	396:407	arg1	diseases					409:416	inflammatory diseases	396:416	inflammatory diseases	396:416	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	12	16	theme	pharmaceutical	1655:1668	arg1	CEP					1626:1628	CEP	1626:1628	CEP	1626:1628	CEP may serve as a potential pharmaceutical candidate in the treatment of UC.
36770726	12	16	theme	pharmaceutical	1655:1668	arg1	candidate					1670:1678	a potential pharmaceutical candidate	1643:1678	a potential pharmaceutical candidate in the treatment of UC	1643:1701	CEP may serve as a potential pharmaceutical candidate in the treatment of UC.
36770726	10	17	theme	inflammation	1510:1521	arg1	severity					1487:1494	the severity	1483:1494	the severity of intestinal inflammation	1483:1521	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	9	18	theme	ACOD1	1264:1268	arg1	level					1281:1285	ACOD1 expression level	1264:1285	ACOD1 expression level	1264:1285	Correlation analysis also revealed that Family-XIII-AD3011-group and Rumini-clostridium-6 were positively correlated with ACOD1 expression level.
36770726	10	19	theme	Microbiome	1332:1341	arg1	iHMP					1352:1355	iHMP	1352:1355	iHMP	1352:1355	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	10	19	theme	Microbiome	1332:1341	arg1	Project					1343:1349	the integrative Human Microbiome Project	1310:1349	the integrative Human Microbiome Project (iHMP)	1310:1356	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	1	20	theme	Stephania	206:214	arg1	tubers					196:201	tubers	196:201	tubers of Stephania	196:214	Cepharanthine (CEP), a bisbenzylisoquinoline alkaloid from tubers of Stephania, protects against some inflammatory diseases.
36770726	2	21	theme	acid	458:461	arg1	production					435:444	the production	431:444	the production of itaconic acid	431:461	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	12	22	theme	potential	1645:1653	arg1	CEP					1626:1628	CEP	1626:1628	CEP	1626:1628	CEP may serve as a potential pharmaceutical candidate in the treatment of UC.
36770726	12	22	theme	potential	1645:1653	arg1	candidate					1670:1678	a potential pharmaceutical candidate	1643:1678	a potential pharmaceutical candidate in the treatment of UC	1643:1701	CEP may serve as a potential pharmaceutical candidate in the treatment of UC.
36770726	10	23	theme	intestinal	1499:1508	arg1	inflammation					1510:1521	intestinal inflammation	1499:1521	intestinal inflammation	1499:1521	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	7	24	theme	colitis	945:951	arg1	model					924:928	the mouse model	914:928	the mouse model of DSS-induced colitis	914:951	Additionally, in the mouse model of DSS-induced colitis, CEP decreased macrophage infiltration and ACOD1 expression in colon tissue.
36770726	0	25	theme	Ulcerative	37:46	arg1	Colitis					48:54	DSS-Induced Ulcerative Colitis	25:54	DSS-Induced Ulcerative Colitis	25:54	Cepharanthine Alleviates DSS-Induced Ulcerative Colitis via Regulating Aconitate Decarboxylase 1 Expression and Macrophage Infiltration.
36770726	9	26	theme	expression	1270:1279	arg1	level					1281:1285	ACOD1 expression level	1264:1285	ACOD1 expression level	1264:1285	Correlation analysis also revealed that Family-XIII-AD3011-group and Rumini-clostridium-6 were positively correlated with ACOD1 expression level.
36770726	12	27	from	candidate	1670:1678	arg1	treatment					1687:1695	the treatment	1683:1695	the treatment of UC	1683:1701	CEP may serve as a potential pharmaceutical candidate in the treatment of UC.
36770726	10	28	theme	UC	1420:1421	arg1	patients					1423:1430	UC patients	1420:1430	UC patients	1420:1430	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	3	29	theme	abnormal	479:486	arg1	expression					488:497	abnormal expression	479:497	abnormal expression in ulcerative colitis (UC)	479:524	ACOD1 exhibits abnormal expression in ulcerative colitis (UC).
36770726	0	30	theme	DSS-Induced	25:35	arg1	Colitis					48:54	DSS-Induced Ulcerative Colitis	25:54	DSS-Induced Ulcerative Colitis	25:54	Cepharanthine Alleviates DSS-Induced Ulcerative Colitis via Regulating Aconitate Decarboxylase 1 Expression and Macrophage Infiltration.
36770726	11	31	theme	gut	1571:1573	arg1	microbiota					1575:1584	gut microbiota	1571:1584	gut microbiota	1571:1584	Collectively, CEP can counter UC by modulating gut microbiota and inhibiting the expression of ACOD1.
36770726	8	32	theme	ACOD1	1088:1092	arg1	expression					1074:1083	the expression	1070:1083	the expression of ACOD1	1070:1092	After treatment with antibiotics (Abx), the expression of ACOD1 changed with the composition of gut microbiota.
36770726	2	33	theme	immune-responsive	313:329	arg1	gene					331:334	immune-responsive gene 1	313:336	immune-responsive gene 1 (IRG1)	313:343	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	2	33	theme	immune-responsive	313:329	arg1	IRG1					339:342	IRG1	339:342	IRG1	339:342	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	2	33	theme	immune-responsive	313:329	arg1	decarboxylase					272:284	Aconitate decarboxylase 1	262:286	Aconitate decarboxylase 1 (ACOD1)	262:294	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	12	34	theme	UC	1700:1701	arg1	treatment					1687:1695	the treatment	1683:1695	the treatment of UC	1683:1701	CEP may serve as a potential pharmaceutical candidate in the treatment of UC.
36770726	5	35	theme	in	720:721	arg1	experiments					741:751	in vitro and in vivo experiments	720:751	in vitro and in vivo experiments	720:751	This study was designed to explore the protective effects and mechanisms of CEP in treating colitis through in vitro and in vivo experiments.
36770726	6	36	theme	RAW264.7	875:882	arg1	macrophages					884:894	RAW264.7 macrophages	875:894	RAW264.7 macrophages	875:894	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	2	37	theme	Aconitate	262:270	arg1	gene					331:334	immune-responsive gene 1	313:336	immune-responsive gene 1 (IRG1)	313:343	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	2	37	theme	Aconitate	262:270	arg1	decarboxylase					272:284	Aconitate decarboxylase 1	262:286	Aconitate decarboxylase 1 (ACOD1)	262:294	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	2	37	theme	Aconitate	262:270	arg1	ACOD1					289:293	ACOD1	289:293	ACOD1	289:293	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	1	38	theme	inflammatory	239:250	arg1	diseases					252:259	some inflammatory diseases	234:259	some inflammatory diseases	234:259	Cepharanthine (CEP), a bisbenzylisoquinoline alkaloid from tubers of Stephania, protects against some inflammatory diseases.
36770726	0	39	theme	Aconitate	71:79	arg1	Expression					97:106	Aconitate Decarboxylase 1 Expression	71:106	Aconitate Decarboxylase 1 Expression	71:106	Cepharanthine Alleviates DSS-Induced Ulcerative Colitis via Regulating Aconitate Decarboxylase 1 Expression and Macrophage Infiltration.
36770726	8	40	with	treatment	1036:1044	arg1	Abx					1064:1066	Abx	1064:1066	Abx	1064:1066	After treatment with antibiotics (Abx), the expression of ACOD1 changed with the composition of gut microbiota.
36770726	8	40	with	treatment	1036:1044	arg1	antibiotics					1051:1061	antibiotics	1051:1061	antibiotics (Abx)	1051:1067	After treatment with antibiotics (Abx), the expression of ACOD1 changed with the composition of gut microbiota.
36770726	2	41	from	role	388:391	arg1	diseases					409:416	inflammatory diseases	396:416	inflammatory diseases	396:416	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	2	42	theme	itaconic	449:456	arg1	acid					458:461	itaconic acid	449:461	itaconic acid	449:461	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	5	43	theme	CEP	688:690	arg1	effects					662:668	the protective effects	647:668	the protective effects	647:668	This study was designed to explore the protective effects and mechanisms of CEP in treating colitis through in vitro and in vivo experiments.
36770726	5	43	theme	CEP	688:690	arg1	mechanisms					674:683	mechanisms	674:683	mechanisms	674:683	This study was designed to explore the protective effects and mechanisms of CEP in treating colitis through in vitro and in vivo experiments.
36770726	1	44	theme	bisbenzylisoquinoline	160:180	arg1	alkaloid					182:189	a bisbenzylisoquinoline alkaloid	158:189	a bisbenzylisoquinoline alkaloid from tubers of Stephania	158:214	Cepharanthine (CEP), a bisbenzylisoquinoline alkaloid from tubers of Stephania, protects against some inflammatory diseases.
36770726	1	44	theme	bisbenzylisoquinoline	160:180	arg1	Cepharanthine					137:149	Cepharanthine	137:149	Cepharanthine (CEP)	137:155	Cepharanthine (CEP), a bisbenzylisoquinoline alkaloid from tubers of Stephania, protects against some inflammatory diseases.
36770726	6	45	theme	In	754:755	arg1	assays					763:768	In vitro assays	754:768	In vitro assays	754:768	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	8	46	theme	microbiota	1130:1139	arg1	composition					1111:1121	the composition	1107:1121	the composition of gut microbiota	1107:1139	After treatment with antibiotics (Abx), the expression of ACOD1 changed with the composition of gut microbiota.
36770726	6	47	theme	ACOD1	855:859	arg1	expression					861:870	ACOD1 expression	855:870	ACOD1 expression in RAW264.7 macrophages	855:894	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	3	48	from	expression	488:497	arg1	colitis					513:519	ulcerative colitis	502:519	ulcerative colitis (UC)	502:524	ACOD1 exhibits abnormal expression in ulcerative colitis (UC).
36770726	3	48	from	expression	488:497	arg1	UC					522:523	UC	522:523	UC	522:523	ACOD1 exhibits abnormal expression in ulcerative colitis (UC).
36770726	10	49	theme	patients	1423:1430	arg1	tissue					1410:1415	the colon tissue	1400:1415	the colon tissue of UC patients	1400:1430	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	7	50	theme	DSS-induced	933:943	arg1	colitis					945:951	DSS-induced colitis	933:951	DSS-induced colitis	933:951	Additionally, in the mouse model of DSS-induced colitis, CEP decreased macrophage infiltration and ACOD1 expression in colon tissue.
36770726	5	51	dep	in	733:734	arg1	vivo					736:739	vivo	736:739	vivo	736:739	This study was designed to explore the protective effects and mechanisms of CEP in treating colitis through in vitro and in vivo experiments.
36770726	7	52	theme	colon	1016:1020	arg1	tissue					1022:1027	colon tissue	1016:1027	colon tissue	1016:1027	Additionally, in the mouse model of DSS-induced colitis, CEP decreased macrophage infiltration and ACOD1 expression in colon tissue.
36770726	3	53	theme	ulcerative	502:511	arg1	colitis					513:519	ulcerative colitis	502:519	ulcerative colitis (UC)	502:524	ACOD1 exhibits abnormal expression in ulcerative colitis (UC).
36770726	3	53	theme	ulcerative	502:511	arg1	UC					522:523	UC	522:523	UC	522:523	ACOD1 exhibits abnormal expression in ulcerative colitis (UC).
36770726	7	54	theme	mouse	918:922	arg1	model					924:928	the mouse model	914:928	the mouse model of DSS-induced colitis	914:951	Additionally, in the mouse model of DSS-induced colitis, CEP decreased macrophage infiltration and ACOD1 expression in colon tissue.
36770726	5	55	dep	in	720:721	arg1	vitro					723:727	vitro	723:727	vitro	723:727	This study was designed to explore the protective effects and mechanisms of CEP in treating colitis through in vitro and in vivo experiments.
36770726	6	56	theme	cytokines	841:849	arg1	secretion					811:819	LPS-induced secretion	799:819	LPS-induced secretion of pro-inflammatory cytokines	799:849	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	6	56	theme	cytokines	841:849	arg1	expression					861:870	ACOD1 expression	855:870	ACOD1 expression in RAW264.7 macrophages	855:894	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	10	57	theme	Human	1326:1330	arg1	iHMP					1352:1355	iHMP	1352:1355	iHMP	1352:1355	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	10	57	theme	Human	1326:1330	arg1	Project					1343:1349	the integrative Human Microbiome Project	1310:1349	the integrative Human Microbiome Project (iHMP)	1310:1356	Additionally, data of the integrative Human Microbiome Project (iHMP) showed that ACOD1 was highly expressed in the colon tissue of UC patients and this expression was positively correlated with the severity of intestinal inflammation.
36770726	2	58	theme	immunometabolism	371:386	arg1	role					388:391	an important immunometabolism role	358:391	an important immunometabolism role in inflammatory diseases	358:416	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	0	59	theme	Decarboxylase	81:93	arg1	Expression					97:106	Aconitate Decarboxylase 1 Expression	71:106	Aconitate Decarboxylase 1 Expression	71:106	Cepharanthine Alleviates DSS-Induced Ulcerative Colitis via Regulating Aconitate Decarboxylase 1 Expression and Macrophage Infiltration.
36770726	1	60	from	tubers	196:201	arg1	alkaloid					182:189	a bisbenzylisoquinoline alkaloid	158:189	a bisbenzylisoquinoline alkaloid from tubers of Stephania	158:214	Cepharanthine (CEP), a bisbenzylisoquinoline alkaloid from tubers of Stephania, protects against some inflammatory diseases.
36770726	1	60	from	tubers	196:201	arg1	Cepharanthine					137:149	Cepharanthine	137:149	Cepharanthine (CEP)	137:155	Cepharanthine (CEP), a bisbenzylisoquinoline alkaloid from tubers of Stephania, protects against some inflammatory diseases.
36770726	2	61	theme	important	361:369	arg1	role					388:391	an important immunometabolism role	358:391	an important immunometabolism role in inflammatory diseases	358:416	Aconitate decarboxylase 1 (ACOD1) is also known as immune-responsive gene 1 (IRG1), which plays an important immunometabolism role in inflammatory diseases by mediating the production of itaconic acid.
36770726	6	62	dep	In	754:755	arg1	vitro					757:761	vitro	757:761	vitro	757:761	In vitro assays indicated that CEP inhibited LPS-induced secretion of pro-inflammatory cytokines and ACOD1 expression in RAW264.7 macrophages.
36770726	5	63	theme	in	733:734	arg1	experiments					741:751	in vitro and in vivo experiments	720:751	in vitro and in vivo experiments	720:751	This study was designed to explore the protective effects and mechanisms of CEP in treating colitis through in vitro and in vivo experiments.
36306140	12	0	theme	beneficial	2702:2711	arg1	effects					2713:2719	The beneficial effects	2698:2719	The beneficial effects of CUR	2698:2726	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	9	1	theme	cells	2261:2265	arg1	cycle					2230:2234	the tricarboxylic acid cycle	2207:2234	the tricarboxylic acid cycle of intestinal epithelial cells	2207:2265	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	4	2	theme	crossbred	834:842	arg1	chickens					844:851	Seven hundred and twenty 1-day-old crossbred chickens	799:851	Seven hundred and twenty 1-day-old crossbred chickens	799:851	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	4	3	theme	basal	1044:1048	arg1	diet					1050:1053	basal diet	1044:1053	basal diet	1044:1053	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	11	4	theme	dysbiosis	2687:2695	arg1	structure					2595:2603	the structure	2591:2603	the structure of the intestinal microbiome for health promotion	2591:2653	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	11	4	theme	dysbiosis	2687:2695	arg1	treatment					2663:2671	the treatment	2659:2671	the treatment of intestinal dysbiosis	2659:2695	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	10	5	theme	sirtuin	2344:2350	arg1	transcription					2354:2366	sirtuin 1 and sirtuin 5 transcription	2330:2366	transcription	2354:2366	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	7	6	theme	mucosal	1680:1686	arg1	immunity					1688:1695	the intestinal mucosal immunity	1665:1695	the intestinal mucosal immunity	1665:1695	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	7	7	dep	cytokines	1756:1764	arg1	interleukin					1733:1743	interleukin 10	1733:1746	interleukin 10	1733:1746	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	7	7	dep	cytokines	1756:1764	arg1	IL-10					1749:1753	IL-10	1749:1753	IL-10	1749:1753	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	6	8	theme	intestinal	1384:1393	arg1	junctions					1401:1409	the intestinal tight junctions	1380:1409	the intestinal tight junctions	1380:1409	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	4	9	theme	basal	916:920	arg1	diet					922:925	basal diet	916:925	basal diet	916:925	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	10	10	theme	metabolic	2438:2446	arg1	responses					2469:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	0	11	theme	lymphoid	138:145	arg1	cells					147:151	group 3 innate lymphoid cells	123:151	group 3 innate lymphoid cells in chickens	123:163	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	1	12	theme	immune	231:236	arg1	homeostasis					238:248	immune homeostasis	231:248	immune homeostasis	231:248	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	11	13	theme	health	2638:2643	arg1	promotion					2645:2653	health promotion	2638:2653	health promotion	2638:2653	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	2	14	theme	potential	525:533	arg1	therapies					535:543	potential therapies	525:543	potential therapies for gastrointestinal diseases	525:573	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	5	15	from	chickens	1159:1166	arg1	CON_LPS					1175:1181	CON_LPS	1175:1181	CON_LPS	1175:1181	On days 14, 17, and 21, the chickens in the CON_LPS and CUR_LPS treatments were intraperitoneally injected with LPS at 0.5 mg per kg BW.
36306140	5	15	from	chickens	1159:1166	arg1	CUR_LPS					1187:1193	CUR_LPS	1187:1193	CUR_LPS	1187:1193	On days 14, 17, and 21, the chickens in the CON_LPS and CUR_LPS treatments were intraperitoneally injected with LPS at 0.5 mg per kg BW.
36306140	6	16	theme	Dietary	1268:1274	arg1	supplementation					1280:1294	Dietary CUR supplementation	1268:1294	Dietary CUR supplementation	1268:1294	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	3	17	theme	-induced	741:748	arg1	imbalance					750:758	lipopolysaccharide (LPS)-induced imbalance	717:758	lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens	717:796	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	6	18	theme	inflammatory	1464:1475	arg1	response					1477:1484	LPS-induced acute inflammatory response	1446:1484	LPS-induced acute inflammatory response	1446:1484	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	10	19	theme	oxidative	2452:2460	arg1	responses					2469:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	4	20	dep	CUR_saline	946:955	arg1	curcumin					983:990	300 mg kg-1 curcumin	971:990	300 mg kg-1 curcumin	971:990	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	4	20	dep	CUR_saline	946:955	arg1	diet					964:967	basal diet	958:967	basal diet	958:967	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	3	21	theme	gut	622:624	arg1	microbiome					626:635	the gut microbiome	618:635	the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens	618:796	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	6	22	theme	LPS-induced	1446:1456	arg1	response					1477:1484	LPS-induced acute inflammatory response	1446:1484	LPS-induced acute inflammatory response	1446:1484	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	11	23	theme	intestinal	2612:2621	arg1	microbiome					2623:2632	the intestinal microbiome	2608:2632	the intestinal microbiome for health promotion	2608:2653	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	8	24	theme	protein-coupled	1928:1942	arg1	member					1969:1974	G protein-coupled receptor class C group 5 member	1926:1974	G protein-coupled receptor class C group 5 member	1926:1974	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	12	25	theme	inflammation	2804:2815	arg1	pathway					2778:2784	the BA-FXR pathway	2767:2784	the BA-FXR pathway	2767:2784	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	12	25	theme	inflammation	2804:2815	arg1	inhibition					2790:2799	inhibition	2790:2799	inhibition of inflammation	2790:2815	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	4	26	theme	mg	1061:1062	arg1	curcumin					1069:1076	300 mg kg-1 curcumin	1057:1076	300 mg kg-1 curcumin	1057:1076	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	3	27	theme	homeostasis	774:784	arg1	imbalance					750:758	lipopolysaccharide (LPS)-induced imbalance	717:758	lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens	717:796	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	0	28	theme	gut	83:85	arg1	structure					98:106	gut microbiota structure	83:106	gut microbiota structure	83:106	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	12	29	theme	CUR	2724:2726	arg1	effects					2713:2719	The beneficial effects	2698:2719	The beneficial effects of CUR	2698:2726	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	1	30	theme	Gastrointestinal	166:181	arg1	dysfunction					183:193	Gastrointestinal dysfunction	166:193	Gastrointestinal dysfunction	166:193	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	7	31	theme	BA	1620:1621	arg1	metabolism					1623:1632	BA metabolism	1620:1632	BA metabolism	1620:1632	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	12	32	theme	BA-FXR	2771:2776	arg1	pathway					2778:2784	the BA-FXR pathway	2767:2784	the BA-FXR pathway	2767:2784	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	10	33	theme	protein	2372:2378	arg1	expression					2380:2389	protein expression	2372:2389	protein expression	2372:2389	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	9	34	theme	CUR	2130:2132	arg1	supplementation					2111:2125	Dietary supplementation	2103:2125	Dietary supplementation of CUR	2103:2132	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	8	35	theme	farnesoid	1895:1903	arg1	FXR					1917:1919	FXR	1917:1919	FXR	1917:1919	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	8	35	theme	farnesoid	1895:1903	arg1	receptor					1907:1914	farnesoid X receptor	1895:1914	farnesoid X receptor (FXR)	1895:1920	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	1	36	theme	group	367:371	arg1	imbalance					354:362	dynamic imbalance	346:362	dynamic imbalance of group 3 innate lymphoid cells (ILC3s)	346:403	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	36	theme	group	367:371	arg1	changes					251:257	changes	251:257	changes in the intestinal microbiome	251:286	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	36	theme	group	367:371	arg1	disturbance					216:226	a disturbance	214:226	a disturbance of immune homeostasis	214:248	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	36	theme	group	367:371	arg1	alteration					289:298	alteration	289:298	alteration of the composition of the bile acid pool	289:339	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	6	37	theme	tumor	1537:1541	arg1	necrosis					1543:1550	tumor necrosis factor-α	1537:1559	tumor necrosis factor-α	1537:1559	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	6	37	theme	tumor	1537:1541	arg1	cytokines					1562:1570	pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines	1500:1570	pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines	1500:1570	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	7	38	theme	cecal	1590:1594	arg1	community					1606:1614	cecal microbial community	1590:1614	cecal microbial community	1590:1614	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	12	39	theme	lamina	2873:2878	arg1	propria					2880:2886	the intestinal lamina propria	2858:2886	the intestinal lamina propria	2858:2886	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	1	40	theme	innate	375:380	arg1	ILC3s					398:402	ILC3s	398:402	ILC3s	398:402	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	40	theme	innate	375:380	arg1	cells					391:395	innate lymphoid cells	375:395	group 3 innate lymphoid cells (ILC3s)	367:403	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	7	41	theme	chenodexycholic	1840:1854	arg1	acid					1856:1859	chenodexycholic acid	1840:1859	chenodexycholic acid	1840:1859	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	7	41	theme	chenodexycholic	1840:1854	arg1	acid					1874:1877	lithocholic acid	1862:1877	lithocholic acid	1862:1877	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	8	42	from	ILC3s	2096:2100	arg1	increase					2045:2052	an increase	2042:2052	an increase in interleukin 22 (IL-22) production from ILC3s	2042:2100	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	8	42	from	ILC3s	2096:2100	arg1	production					2080:2089	interleukin 22 (IL-22) production	2057:2089	interleukin 22 (IL-22) production from ILC3s	2057:2100	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	8	43	theme	group	1961:1965	arg1	member					1969:1974	G protein-coupled receptor class C group 5 member	1926:1974	G protein-coupled receptor class C group 5 member	1926:1974	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	2	44	theme	polyphenolic	424:435	arg1	Curcumin					406:413	Curcumin	406:413	Curcumin (CUR)	406:419	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	2	44	theme	polyphenolic	424:435	arg1	compound					437:444	a polyphenolic compound	422:444	a polyphenolic compound isolated from turmeric	422:467	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	0	45	theme	microbiota	87:96	arg1	structure					98:106	gut microbiota structure	83:106	gut microbiota structure	83:106	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	6	46	theme	CUR	1276:1278	arg1	supplementation					1280:1294	Dietary CUR supplementation	1268:1294	Dietary CUR supplementation	1268:1294	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	7	47	theme	metabolites	1827:1837	arg1	concentrations					1784:1797	the concentrations	1780:1797	the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid)	1780:1878	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	6	48	theme	pro-inflammatory	1500:1515	arg1	interleukin-1β					1518:1531	interleukin-1β	1518:1531	interleukin-1β	1518:1531	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	6	48	theme	pro-inflammatory	1500:1515	arg1	necrosis					1543:1550	tumor necrosis factor-α	1537:1559	tumor necrosis factor-α	1537:1559	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	6	48	theme	pro-inflammatory	1500:1515	arg1	cytokines					1562:1570	pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines	1500:1570	pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines	1500:1570	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	0	49	theme	LPS-induced	20:30	arg1	imbalance					55:63	LPS-induced intestinal homeostatic imbalance	20:63	LPS-induced intestinal homeostatic imbalance	20:63	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	7	50	theme	secondary	1814:1822	arg1	metabolites					1827:1837	primary and secondary BA metabolites	1802:1837	primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid)	1802:1878	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	4	51	theme	300	971:973	arg1	mg					975:976	mg	975:976	mg	975:976	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	3	52	theme	bile	649:652	arg1	BA					660:661	BA	660:661	BA	660:661	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	3	52	theme	bile	649:652	arg1	acid					654:657	bile acid	649:657	bile acid (BA) metabolism	649:673	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	0	53	theme	homeostatic	43:53	arg1	imbalance					55:63	LPS-induced intestinal homeostatic imbalance	20:63	LPS-induced intestinal homeostatic imbalance	20:63	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	1	54	theme	bile	326:329	arg1	pool					336:339	the bile acid pool	322:339	the bile acid pool	322:339	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	7	55	theme	primary	1802:1808	arg1	metabolites					1827:1837	primary and secondary BA metabolites	1802:1837	primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid)	1802:1878	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	4	56	theme	kg-1	978:981	arg1	curcumin					983:990	300 mg kg-1 curcumin	971:990	300 mg kg-1 curcumin	971:990	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	8	57	theme	class	1953:1957	arg1	member					1969:1974	G protein-coupled receptor class C group 5 member	1926:1974	G protein-coupled receptor class C group 5 member	1926:1974	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	1	58	theme	pool	336:339	arg1	composition					307:317	the composition	303:317	the composition of the bile acid pool	303:339	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	9	59	theme	acid	2225:2228	arg1	cycle					2230:2234	the tricarboxylic acid cycle	2207:2234	the tricarboxylic acid cycle of intestinal epithelial cells	2207:2265	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	6	60	theme	performance	1354:1364	arg1	suppression					1332:1342	LPS-induced suppression	1320:1342	LPS-induced suppression of growth performance and injury	1320:1375	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	8	61	theme	CUR	2011:2013	arg1	administration					2015:2028	CUR administration	2011:2028	CUR administration	2011:2028	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	12	62	theme	IL-22	2830:2834	arg1	secretion					2836:2844	IL-22 secretion	2830:2844	IL-22 secretion by ILC3s	2830:2853	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	9	63	theme	Dietary	2103:2109	arg1	supplementation					2111:2125	Dietary supplementation	2103:2125	Dietary supplementation of CUR	2103:2132	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	7	64	theme	anti-inflammatory	1714:1730	arg1	cytokines					1756:1764	the anti-inflammatory (interleukin 10, IL-10) cytokines	1710:1764	the anti-inflammatory (interleukin 10, IL-10) cytokines	1710:1764	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	9	65	theme	epithelial	2250:2259	arg1	cells					2261:2265	intestinal epithelial cells	2239:2265	intestinal epithelial cells	2239:2265	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	6	66	theme	LPS-induced	1320:1330	arg1	suppression					1332:1342	LPS-induced suppression	1320:1342	LPS-induced suppression of growth performance and injury	1320:1375	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	8	67	theme	X	1905:1905	arg1	FXR					1917:1919	FXR	1917:1919	FXR	1917:1919	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	8	67	theme	X	1905:1905	arg1	receptor					1907:1914	farnesoid X receptor	1895:1914	farnesoid X receptor (FXR)	1895:1920	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	8	68	dep	receptor	1907:1914	arg1	synthesis					1978:1986	A synthesis	1976:1986	A synthesis	1976:1986	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	4	69	theme	basal	1012:1016	arg1	diet					1018:1021	basal diet	1012:1021	basal diet	1012:1021	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	7	70	theme	immunity	1688:1695	arg1	regulation					1651:1660	regulation	1651:1660	regulation of the intestinal mucosal immunity	1651:1695	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	4	71	theme	1-day-old	824:832	arg1	chickens					844:851	Seven hundred and twenty 1-day-old crossbred chickens	799:851	Seven hundred and twenty 1-day-old crossbred chickens	799:851	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	7	72	theme	intestinal	1669:1678	arg1	immunity					1688:1695	the intestinal mucosal immunity	1665:1695	the intestinal mucosal immunity	1665:1695	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	8	73	theme	interleukin	2057:2067	arg1	production					2080:2089	interleukin 22 (IL-22) production	2057:2089	interleukin 22 (IL-22) production from ILC3s	2057:2100	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	4	74	theme	300	1057:1059	arg1	mg					1061:1062	mg	1061:1062	mg	1061:1062	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	3	75	theme	ILC3s	695:699	arg1	metabolism					664:673	bile acid (BA) metabolism	649:673	bile acid (BA) metabolism	649:673	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	3	75	theme	ILC3s	695:699	arg1	function					683:690	the function	679:690	the function of ILC3s	679:699	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	6	76	theme	tight	1395:1399	arg1	junctions					1401:1409	the intestinal tight junctions	1380:1409	the intestinal tight junctions	1380:1409	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	6	77	dep	cytokines	1562:1570	arg1	interleukin-1β					1518:1531	interleukin-1β	1518:1531	interleukin-1β	1518:1531	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	6	77	dep	cytokines	1562:1570	arg1	necrosis					1543:1550	tumor necrosis factor-α	1537:1559	tumor necrosis factor-α	1537:1559	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	6	77	dep	cytokines	1562:1570	arg1	cytokines					1562:1570	pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines	1500:1570	pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines	1500:1570	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	11	78	theme	intestinal	2676:2685	arg1	dysbiosis					2687:2695	intestinal dysbiosis	2676:2695	intestinal dysbiosis	2676:2695	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	1	79	from	alteration	289:298	arg1	microbiome					277:286	the intestinal microbiome	262:286	the intestinal microbiome	262:286	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	10	80	theme	mitochondrial	2424:2436	arg1	responses					2469:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	6	81	theme	injury	1370:1375	arg1	suppression					1332:1342	LPS-induced suppression	1320:1342	LPS-induced suppression of growth performance and injury	1320:1375	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	1	82	theme	homeostasis	238:248	arg1	imbalance					354:362	dynamic imbalance	346:362	dynamic imbalance of group 3 innate lymphoid cells (ILC3s)	346:403	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	82	theme	homeostasis	238:248	arg1	changes					251:257	changes	251:257	changes in the intestinal microbiome	251:286	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	82	theme	homeostasis	238:248	arg1	disturbance					216:226	a disturbance	214:226	a disturbance of immune homeostasis	214:248	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	82	theme	homeostasis	238:248	arg1	alteration					289:298	alteration	289:298	alteration of the composition of the bile acid pool	289:339	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	3	83	theme	intestinal	763:772	arg1	homeostasis					774:784	intestinal homeostasis	763:784	intestinal homeostasis	763:784	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	4	84	dep	CUR_LPS	1035:1041	arg1	curcumin					1069:1076	300 mg kg-1 curcumin	1057:1076	300 mg kg-1 curcumin	1057:1076	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	4	84	dep	CUR_LPS	1035:1041	arg1	diet					1050:1053	basal diet	1044:1053	basal diet	1044:1053	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	10	85	theme	stress	2462:2467	arg1	responses					2469:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	mitochondrial metabolic and oxidative stress responses	2424:2477	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	1	86	from	changes	251:257	arg1	microbiome					277:286	the intestinal microbiome	262:286	the intestinal microbiome	262:286	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	6	87	theme	acute	1458:1462	arg1	response					1477:1484	LPS-induced acute inflammatory response	1446:1484	LPS-induced acute inflammatory response	1446:1484	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	2	88	theme	gastrointestinal	549:564	arg1	diseases					566:573	gastrointestinal diseases	549:573	gastrointestinal diseases	549:573	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	2	89	from	inflammation	509:520	arg1	therapies					535:543	potential therapies	525:543	potential therapies for gastrointestinal diseases	525:573	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	4	90	theme	kg-1	1064:1067	arg1	curcumin					1069:1076	300 mg kg-1 curcumin	1057:1076	300 mg kg-1 curcumin	1057:1076	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	11	91	theme	microbiome	2623:2632	arg1	structure					2595:2603	the structure	2591:2603	the structure of the intestinal microbiome for health promotion	2591:2653	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	11	91	theme	microbiome	2623:2632	arg1	treatment					2663:2671	the treatment	2659:2671	the treatment of intestinal dysbiosis	2659:2695	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	12	92	theme	intestinal	2862:2871	arg1	propria					2880:2886	the intestinal lamina propria	2858:2886	the intestinal lamina propria	2858:2886	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	4	93	dep	CON_saline	904:913	arg1	diet					922:925	basal diet	916:925	basal diet	916:925	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	4	93	dep	CON_saline	904:913	arg1	control					936:942	saline control	929:942	saline control	929:942	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	8	94	from	increase	2045:2052	arg1	ILC3s					2096:2100	ILC3s	2096:2100	ILC3s	2096:2100	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	8	94	from	increase	2045:2052	arg1	production					2080:2089	interleukin 22 (IL-22) production	2057:2089	interleukin 22 (IL-22) production from ILC3s	2057:2100	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	12	95	theme	inhibition	2790:2799	arg1	modulation					2753:2762	the modulation	2749:2762	the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria	2749:2886	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	10	96	theme	LPS-induced	2492:2502	arg1	enteritis					2504:2512	LPS-induced enteritis	2492:2512	LPS-induced enteritis	2492:2512	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	4	97	theme	saline	929:934	arg1	control					936:942	saline control	929:942	saline control	929:942	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	1	98	theme	dynamic	346:352	arg1	imbalance					354:362	dynamic imbalance	346:362	dynamic imbalance of group 3 innate lymphoid cells (ILC3s)	346:403	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	98	theme	dynamic	346:352	arg1	disturbance					216:226	a disturbance	214:226	a disturbance of immune homeostasis	214:248	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	7	99	theme	lithocholic	1862:1872	arg1	acid					1856:1859	chenodexycholic acid	1840:1859	chenodexycholic acid	1840:1859	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	7	99	theme	lithocholic	1862:1872	arg1	acid					1874:1877	lithocholic acid	1862:1877	lithocholic acid	1862:1877	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	4	100	theme	basal	958:962	arg1	diet					964:967	basal diet	958:967	basal diet	958:967	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	3	101	from	imbalance	750:758	arg1	chickens					789:796	chickens	789:796	chickens	789:796	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	0	102	theme	group	123:127	arg1	cells					147:151	group 3 innate lymphoid cells	123:151	group 3 innate lymphoid cells in chickens	123:163	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	4	103	dep	CON_LPS	1003:1009	arg1	LPS					1025:1027	LPS	1025:1027	LPS	1025:1027	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	4	103	dep	CON_LPS	1003:1009	arg1	diet					1018:1021	basal diet	1012:1021	basal diet	1012:1021	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	2	104	theme	intestinal	498:507	arg1	inflammation					509:520	intestinal inflammation	498:520	intestinal inflammation in potential therapies for gastrointestinal diseases	498:573	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	0	105	theme	innate	131:136	arg1	cells					147:151	group 3 innate lymphoid cells	123:151	group 3 innate lymphoid cells in chickens	123:163	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	2	106	attach	isolated	446:453	arg2	Curcumin					406:413	Curcumin	406:413	Curcumin (CUR)	406:419	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	2	106	attach	isolated	446:453	arg2	compound					437:444	a polyphenolic compound	422:444	a polyphenolic compound isolated from turmeric	422:467	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	2	106	attach	isolated	446:453	arg1	turmeric					460:467	turmeric	460:467	turmeric	460:467	Curcumin (CUR), a polyphenolic compound isolated from turmeric, has been known to attenuate intestinal inflammation in potential therapies for gastrointestinal diseases.
36306140	7	107	theme	microbial	1596:1604	arg1	community					1606:1614	cecal microbial community	1590:1614	cecal microbial community	1590:1614	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	10	108	theme	curcumin	2281:2288	arg1	supplementation					2290:2304	curcumin supplementation	2281:2304	curcumin supplementation	2281:2304	In addition, curcumin supplementation significantly increased sirtuin 1 and sirtuin 5 transcription and protein expression, which contributes to regulating mitochondrial metabolic and oxidative stress responses to alleviate LPS-induced enteritis.
36306140	7	109	dep	community	1606:1614	arg1	the					1586:1588	the	1586:1588	the	1586:1588	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	1	110	theme	lymphoid	382:389	arg1	ILC3s					398:402	ILC3s	398:402	ILC3s	398:402	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	110	theme	lymphoid	382:389	arg1	cells					391:395	innate lymphoid cells	375:395	group 3 innate lymphoid cells (ILC3s)	367:403	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	7	111	theme	BA	1824:1825	arg1	metabolites					1827:1837	primary and secondary BA metabolites	1802:1837	primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid)	1802:1878	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	9	112	theme	Butyricicoccus	2162:2175	arg1	prevalence					2148:2157	the prevalence	2144:2157	the prevalence of Butyricicoccus and Enterococcus	2144:2192	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	0	113	theme	intestinal	32:41	arg1	imbalance					55:63	LPS-induced intestinal homeostatic imbalance	20:63	LPS-induced intestinal homeostatic imbalance	20:63	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	1	114	theme	composition	307:317	arg1	imbalance					354:362	dynamic imbalance	346:362	dynamic imbalance of group 3 innate lymphoid cells (ILC3s)	346:403	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	114	theme	composition	307:317	arg1	changes					251:257	changes	251:257	changes in the intestinal microbiome	251:286	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	114	theme	composition	307:317	arg1	disturbance					216:226	a disturbance	214:226	a disturbance of immune homeostasis	214:248	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	114	theme	composition	307:317	arg1	alteration					289:298	alteration	289:298	alteration of the composition of the bile acid pool	289:339	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	11	115	theme	pivotal	2564:2570	arg1	role					2572:2575	a pivotal role	2562:2575	a pivotal role	2562:2575	Our findings demonstrated that curcumin played a pivotal role in regulating the structure of the intestinal microbiome for health promotion and the treatment of intestinal dysbiosis.
36306140	9	116	theme	Enterococcus	2181:2192	arg1	prevalence					2148:2157	the prevalence	2144:2157	the prevalence of Butyricicoccus and Enterococcus	2144:2192	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	1	117	from	imbalance	354:362	arg1	microbiome					277:286	the intestinal microbiome	262:286	the intestinal microbiome	262:286	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	7	118	dep	metabolites	1827:1837	arg1	acid					1856:1859	chenodexycholic acid	1840:1859	chenodexycholic acid	1840:1859	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	7	118	dep	metabolites	1827:1837	arg1	acid					1874:1877	lithocholic acid	1862:1877	lithocholic acid	1862:1877	CUR reshaped the cecal microbial community and BA metabolism, contributing to regulation of the intestinal mucosal immunity by promoting the anti-inflammatory (interleukin 10, IL-10) cytokines and enhancing the concentrations of primary and secondary BA metabolites (chenodexycholic acid, lithocholic acid).
36306140	12	119	theme	pathway	2778:2784	arg1	modulation					2753:2762	the modulation	2749:2762	the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria	2749:2886	The beneficial effects of CUR may be attributed to the modulation of the BA-FXR pathway and inhibition of inflammation that induces IL-22 secretion by ILC3s in the intestinal lamina propria.
36306140	8	120	theme	C	1959:1959	arg1	member					1969:1974	G protein-coupled receptor class C group 5 member	1926:1974	G protein-coupled receptor class C group 5 member	1926:1974	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	1	121	theme	acid	331:334	arg1	pool					336:339	the bile acid pool	322:339	the bile acid pool	322:339	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	8	122	theme	receptor	1944:1951	arg1	member					1969:1974	G protein-coupled receptor class C group 5 member	1926:1974	G protein-coupled receptor class C group 5 member	1926:1974	LPS decreased farnesoid X receptor (FXR) and G protein-coupled receptor class C group 5 member A synthesis, which was reversed by CUR administration, along with an increase in interleukin 22 (IL-22) production from ILC3s.
36306140	4	123	theme	mg	975:976	arg1	curcumin					983:990	300 mg kg-1 curcumin	971:990	300 mg kg-1 curcumin	971:990	Seven hundred and twenty 1-day-old crossbred chickens were randomly divided into four treatments, namely CON_saline (basal diet + saline control), CUR_saline (basal diet + 300 mg kg-1 curcumin + saline), CON_LPS (basal diet + LPS), and CUR_LPS (basal diet + 300 mg kg-1 curcumin + LPS), each consisting of 6 replicates of 30 birds.
36306140	1	124	dep	group	367:371	arg1	ILC3s					398:402	ILC3s	398:402	ILC3s	398:402	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	1	124	dep	group	367:371	arg1	cells					391:395	innate lymphoid cells	375:395	group 3 innate lymphoid cells (ILC3s)	367:403	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36306140	9	125	theme	tricarboxylic	2211:2223	arg1	cycle					2230:2234	the tricarboxylic acid cycle	2207:2234	the tricarboxylic acid cycle of intestinal epithelial cells	2207:2265	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	5	126	dep	CON_LPS	1175:1181	arg1	treatments					1195:1204	treatments	1195:1204	treatments	1195:1204	On days 14, 17, and 21, the chickens in the CON_LPS and CUR_LPS treatments were intraperitoneally injected with LPS at 0.5 mg per kg BW.
36306140	5	126	dep	CON_LPS	1175:1181	arg1	the					1171:1173	the	1171:1173	the	1171:1173	On days 14, 17, and 21, the chickens in the CON_LPS and CUR_LPS treatments were intraperitoneally injected with LPS at 0.5 mg per kg BW.
36306140	3	127	theme	acid	654:657	arg1	metabolism					664:673	bile acid (BA) metabolism	649:673	bile acid (BA) metabolism	649:673	It was hypothesized that CUR could target the gut microbiome to modulate bile acid (BA) metabolism and the function of ILC3s in ameliorating lipopolysaccharide (LPS)-induced imbalance of intestinal homeostasis in chickens.
36306140	6	128	theme	growth	1347:1352	arg1	performance					1354:1364	growth performance	1347:1364	growth performance	1347:1364	Dietary CUR supplementation significantly decreased LPS-induced suppression of growth performance and injury to the intestinal tight junctions and decreased the vulnerability to LPS-induced acute inflammatory response by inhibiting pro-inflammatory (interleukin-1β and tumor necrosis factor-α) cytokines.
36306140	0	129	from	cells	147:151	arg1	chickens					156:163	chickens	156:163	chickens	156:163	Curcumin alleviates LPS-induced intestinal homeostatic imbalance through reshaping gut microbiota structure and regulating group 3 innate lymphoid cells in chickens.
36306140	9	130	theme	intestinal	2239:2248	arg1	cells					2261:2265	intestinal epithelial cells	2239:2265	intestinal epithelial cells	2239:2265	Dietary supplementation of CUR increased the prevalence of Butyricicoccus and Enterococcus and enhanced the tricarboxylic acid cycle of intestinal epithelial cells.
36306140	1	131	theme	intestinal	266:275	arg1	microbiome					277:286	the intestinal microbiome	262:286	the intestinal microbiome	262:286	Gastrointestinal dysfunction is associated with a disturbance of immune homeostasis, changes in the intestinal microbiome, alteration of the composition of the bile acid pool, and dynamic imbalance of group 3 innate lymphoid cells (ILC3s).
36312959	0	0	theme	colorectal	32:41	arg1	cancers					43:49	colorectal cancers	32:49	colorectal cancers	32:49	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	11	1	theme	pMMR	1563:1566	arg1	group					1568:1572	the pMMR group	1559:1572	the pMMR group	1559:1572	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	13	2	theme	glycan	1896:1901	arg1	pathways					1884:1891	the biosynthesis and metabolic pathways	1853:1891	pathways	1884:1891	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	2	theme	glycan	1896:1901	arg1	pathways					1982:1989	repair pathways	1975:1989	repair pathways	1975:1989	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	2	theme	glycan	1896:1901	arg1	growth					1924:1929	cell growth	1919:1929	cell growth	1919:1929	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	2	theme	glycan	1896:1901	arg1	pathways					1941:1948	death pathways	1935:1948	death pathways	1935:1948	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	2	theme	glycan	1896:1901	arg1	replication					1959:1969	genetic replication	1951:1969	genetic replication	1951:1969	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	6	3	theme	LEfSe	866:870	arg1	analysis					873:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	13	4	theme	death	1935:1939	arg1	pathways					1941:1948	death pathways	1935:1948	death pathways	1935:1948	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	14	5	theme	community	2136:2144	arg1	richness					2146:2153	distinct gut bacterial community richness	2113:2153	distinct gut bacterial community richness	2113:2153	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	13	6	theme	nucleotide	1907:1916	arg1	pathways					1884:1891	the biosynthesis and metabolic pathways	1853:1891	pathways	1884:1891	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	6	theme	nucleotide	1907:1916	arg1	pathways					1982:1989	repair pathways	1975:1989	repair pathways	1975:1989	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	6	theme	nucleotide	1907:1916	arg1	growth					1924:1929	cell growth	1919:1929	cell growth	1919:1929	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	6	theme	nucleotide	1907:1916	arg1	pathways					1941:1948	death pathways	1935:1948	death pathways	1935:1948	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	6	theme	nucleotide	1907:1916	arg1	replication					1959:1969	genetic replication	1951:1969	genetic replication	1951:1969	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	3	7	theme	MMR	509:511	arg1	proteins					513:520	four major MMR proteins	498:520	four major MMR proteins	498:520	In this study, we used immunohistochemistry for four major MMR proteins to determine the MMR status in 230 CRC patients.
36312959	8	8	theme	microbiome	1088:1097	arg1	higher					1124:1129	higher	1124:1129	higher	1124:1129	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	8	8	theme	microbiome	1088:1097	arg1	alpha-diversity					1064:1078	alpha-diversity	1064:1078	alpha-diversity	1064:1078	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	8	8	theme	microbiome	1088:1097	arg1	richness					1054:1061	The richness	1050:1061	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue	1050:1118	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	6	9	theme	size	860:863	arg1	analysis					873:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	4	10	theme	cancerous	606:614	arg1	tissues					636:642	cancerous and adjacent normal tissues	606:642	cancerous and adjacent normal tissues	606:642	The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing.
36312959	14	11	theme	gut	2122:2124	arg1	richness					2146:2153	distinct gut bacterial community richness	2113:2153	distinct gut bacterial community richness	2113:2153	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	8	12	theme	dMMR	1102:1105	arg1	tissue					1113:1118	dMMR tumor tissue	1102:1118	dMMR tumor tissue	1102:1118	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	6	13	theme	analysis	844:851	arg1	analysis					873:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	4	14	theme	adjacent	620:627	arg1	tissues					636:642	cancerous and adjacent normal tissues	606:642	cancerous and adjacent normal tissues	606:642	The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing.
36312959	10	15	theme	genus	1537:1541	arg1	level					1543:1547	the genus level	1533:1547	the genus level	1533:1547	The dMMR group was enriched considerably for some microbiota, including Fusobacteria, Firmicutes, Verrucomicrobia, and Actinobacteria at the phylum level and Fusobacterium, Akkermansia, Bifidobacterium, Faecalibacterium, Streptococcus, and Prevotella bacteria at the genus level.
36312959	13	16	theme	pathways	1884:1891	arg1	abundance					1840:1848	a higher abundance	1831:1848	a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples	1831:2005	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	1	17	theme	DNA	168:170	arg1	repair					181:186	deficient DNA mismatch repair	158:186	deficient DNA mismatch repair (dMMR)	158:193	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	17	theme	DNA	168:170	arg1	dMMR					189:192	dMMR	189:192	dMMR	189:192	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	6	18	theme	taxonomic	930:938	arg1	differences					940:950	the significant taxonomic differences	914:950	the significant taxonomic differences	914:950	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	14	19	theme	MMR	2097:2099	arg1	status					2101:2106	different MMR status	2087:2106	different MMR status	2087:2106	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	0	20	theme	230	109:111	arg1	patients					117:124	230 CRC patients	109:124	230 CRC patients	109:124	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	5	21	from	differences	688:698	arg1	diversity					714:722	microbiota diversity	703:722	microbiota diversity	703:722	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	5	21	from	differences	688:698	arg1	pathways					759:766	related metabolic pathways	741:766	related metabolic pathways	741:766	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	5	21	from	differences	688:698	arg1	composition					725:735	composition	725:735	composition	725:735	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	7	22	dep	230	1029:1031	arg1	%					1039:1039	12.61%	1034:1039	12.61%	1034:1039	In our study cohort, dMMR status was identified in 29 of 230 (12.61%) tumors.
36312959	0	23	theme	patients	117:124	arg1	study					100:104	A study	98:104	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.	0:125	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	4	24	theme	16S	663:665	arg1	sequencing					672:681	bacterial 16S rRNA sequencing	653:681	bacterial 16S rRNA sequencing	653:681	The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing.
36312959	14	25	theme	CRC	2069:2071	arg1	patients					2073:2080	CRC patients	2069:2080	CRC patients with different MMR status	2069:2106	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	13	26	theme	pMMR	2025:2028	arg1	group					2030:2034	the pMMR group	2021:2034	the pMMR group	2021:2034	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	2	27	with	CRCs	418:421	arg1	status					442:447	different MMR status	428:447	different MMR status	428:447	However, it is unclear whether gut microbiota is distinct between CRCs with different MMR status.
36312959	11	28	theme	genus	1675:1679	arg1	level					1681:1685	the genus level	1671:1685	the genus level	1671:1685	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	9	29	theme	community	1179:1187	arg1	different					1259:1267	different	1259:1267	different	1259:1267	The microbial community composition (beta-diversity) between the two groups was significantly different.
36312959	9	29	theme	community	1179:1187	arg1	beta-diversity					1202:1215	beta-diversity	1202:1215	beta-diversity	1202:1215	The microbial community composition (beta-diversity) between the two groups was significantly different.
36312959	9	29	theme	community	1179:1187	arg1	composition					1189:1199	The microbial community composition	1165:1199	The microbial community composition (beta-diversity) between the two groups	1165:1239	The microbial community composition (beta-diversity) between the two groups was significantly different.
36312959	13	30	theme	biosynthesis	1857:1868	arg1	pathways					1884:1891	the biosynthesis and metabolic pathways	1853:1891	pathways	1884:1891	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	30	theme	biosynthesis	1857:1868	arg1	pathways					1982:1989	repair pathways	1975:1989	repair pathways	1975:1989	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	30	theme	biosynthesis	1857:1868	arg1	replication					1959:1969	genetic replication	1951:1969	genetic replication	1951:1969	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	31	theme	dMMR	1994:1997	arg1	samples					1999:2005	dMMR samples	1994:2005	dMMR samples	1994:2005	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	5	32	theme	related	741:747	arg1	pathways					759:766	related metabolic pathways	741:766	related metabolic pathways	741:766	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	0	33	theme	mismatch	81:88	arg1	repair					90:95	deficient and proficient mismatch repair	56:95	deficient and proficient mismatch repair	56:95	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	6	34	theme	genus	959:963	arg1	level					965:969	the genus level	955:969	the genus level	955:969	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	13	35	theme	higher	1833:1838	arg1	abundance					1840:1848	a higher abundance	1831:1848	a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples	1831:2005	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	1	36	theme	different	323:331	arg1	outcomes					342:349	different survival outcomes	323:349	different survival outcomes	323:349	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	37	with	cancers	138:144	arg1	repair					223:228	proficient DNA mismatch repair	199:228	proficient DNA mismatch repair (pMMR)	199:235	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	37	with	cancers	138:144	arg1	dMMR					189:192	dMMR	189:192	dMMR	189:192	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	37	with	cancers	138:144	arg1	pMMR					231:234	pMMR	231:234	pMMR	231:234	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	37	with	cancers	138:144	arg1	repair					181:186	deficient DNA mismatch repair	158:186	deficient DNA mismatch repair (dMMR)	158:193	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	0	38	theme	proficient	70:79	arg1	repair					90:95	deficient and proficient mismatch repair	56:95	deficient and proficient mismatch repair	56:95	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	13	39	theme	pathway	1804:1810	arg1	analysis					1812:1819	KEGG pathway analysis	1799:1819	KEGG pathway analysis	1799:1819	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	0	40	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota distinct	0:22	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.	0:125	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	3	41	from	status	543:548	arg1	patients					561:568	230 CRC patients	553:568	230 CRC patients	553:568	In this study, we used immunohistochemistry for four major MMR proteins to determine the MMR status in 230 CRC patients.
36312959	0	42	theme	distinct	15:22	arg1	microbiota					4:13	Gut microbiota distinct	0:22	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.	0:125	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	1	43	theme	heterogeneous	245:257	arg1	characteristics					265:279	heterogeneous tumor characteristics	245:279	heterogeneous tumor characteristics	245:279	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	6	44	theme	discriminant	831:842	arg1	analysis					844:851	Linear discriminant analysis	824:851	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	14	45	theme	metabolic	2181:2189	arg1	pathways					2191:2198	related metabolic pathways	2173:2198	related metabolic pathways	2173:2198	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	8	46	theme	tumor	1150:1154	arg1	tissues					1156:1162	pMMR tumor tissues	1145:1162	pMMR tumor tissues	1145:1162	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	10	47	dep	Firmicutes	1356:1365	arg1	bacteria					1521:1528	bacteria	1521:1528	bacteria	1521:1528	The dMMR group was enriched considerably for some microbiota, including Fusobacteria, Firmicutes, Verrucomicrobia, and Actinobacteria at the phylum level and Fusobacterium, Akkermansia, Bifidobacterium, Faecalibacterium, Streptococcus, and Prevotella bacteria at the genus level.
36312959	8	48	from	richness	1054:1061	arg1	tissue					1113:1118	dMMR tumor tissue	1102:1118	dMMR tumor tissue	1102:1118	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	12	49	theme	functional	1738:1747	arg1	pathways					1749:1756	microbiota associated functional pathways	1716:1756	microbiota associated functional pathways	1716:1756	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	2	50	theme	different	428:436	arg1	status					442:447	different MMR status	428:447	different MMR status	428:447	However, it is unclear whether gut microbiota is distinct between CRCs with different MMR status.
36312959	1	51	theme	distinct	282:289	arg1	responses					291:299	distinct responses	282:299	distinct responses to immunotherapy	282:316	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	12	52	theme	different	1777:1785	arg1	status					1791:1796	different MMR status	1777:1796	different MMR status	1777:1796	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	14	53	contain	have	2108:2111	arg2	compositions					2156:2167	compositions	2156:2167	compositions	2156:2167	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	14	53	contain	have	2108:2111	arg2	richness					2146:2153	distinct gut bacterial community richness	2113:2153	distinct gut bacterial community richness	2113:2153	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	14	53	contain	have	2108:2111	arg1	patients					2073:2080	CRC patients	2069:2080	CRC patients with different MMR status	2069:2106	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	14	53	contain	have	2108:2111	arg2	pathways					2191:2198	related metabolic pathways	2173:2198	related metabolic pathways	2173:2198	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	14	54	theme	related	2173:2179	arg1	pathways					2191:2198	related metabolic pathways	2173:2198	related metabolic pathways	2173:2198	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	13	55	theme	growth	1924:1929	arg1	abundance					1840:1848	a higher abundance	1831:1848	a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples	1831:2005	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	1	56	theme	proficient	199:208	arg1	repair					223:228	proficient DNA mismatch repair	199:228	proficient DNA mismatch repair (pMMR)	199:235	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	56	theme	proficient	199:208	arg1	pMMR					231:234	pMMR	231:234	pMMR	231:234	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	0	57	theme	deficient	56:64	arg1	repair					90:95	deficient and proficient mismatch repair	56:95	deficient and proficient mismatch repair	56:95	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	12	58	theme	microbiota	1716:1725	arg1	pathways					1749:1756	microbiota associated functional pathways	1716:1756	microbiota associated functional pathways	1716:1756	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	10	59	theme	dMMR	1274:1277	arg1	group					1279:1283	The dMMR group	1270:1283	The dMMR group	1270:1283	The dMMR group was enriched considerably for some microbiota, including Fusobacteria, Firmicutes, Verrucomicrobia, and Actinobacteria at the phylum level and Fusobacterium, Akkermansia, Bifidobacterium, Faecalibacterium, Streptococcus, and Prevotella bacteria at the genus level.
36312959	1	60	theme	mismatch	214:221	arg1	repair					223:228	proficient DNA mismatch repair	199:228	proficient DNA mismatch repair (pMMR)	199:235	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	60	theme	mismatch	214:221	arg1	pMMR					231:234	pMMR	231:234	pMMR	231:234	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	5	61	dep	dMMR	790:793	arg1	CRCs					804:807	CRCs	804:807	CRCs	804:807	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	3	62	theme	major	503:507	arg1	proteins					513:520	four major MMR proteins	498:520	four major MMR proteins	498:520	In this study, we used immunohistochemistry for four major MMR proteins to determine the MMR status in 230 CRC patients.
36312959	14	63	theme	bacterial	2126:2134	arg1	richness					2146:2153	distinct gut bacterial community richness	2113:2153	distinct gut bacterial community richness	2113:2153	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	8	64	theme	gut	1084:1086	arg1	microbiome					1088:1097	gut microbiome	1084:1097	gut microbiome	1084:1097	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	6	65	theme	effect	853:858	arg1	analysis					873:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	8	66	theme	pMMR	1145:1148	arg1	tissues					1156:1162	pMMR tumor tissues	1145:1162	pMMR tumor tissues	1145:1162	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	14	67	theme	distinct	2113:2120	arg1	richness					2146:2153	distinct gut bacterial community richness	2113:2153	distinct gut bacterial community richness	2113:2153	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	13	68	theme	metabolic	1874:1882	arg1	pathways					1884:1891	the biosynthesis and metabolic pathways	1853:1891	pathways	1884:1891	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	68	theme	metabolic	1874:1882	arg1	pathways					1982:1989	repair pathways	1975:1989	repair pathways	1975:1989	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	68	theme	metabolic	1874:1882	arg1	replication					1959:1969	genetic replication	1951:1969	genetic replication	1951:1969	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	1	69	theme	deficient	158:166	arg1	repair					181:186	deficient DNA mismatch repair	158:186	deficient DNA mismatch repair (dMMR)	158:193	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	69	theme	deficient	158:166	arg1	dMMR					189:192	dMMR	189:192	dMMR	189:192	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	0	70	theme	CRC	113:115	arg1	patients					117:124	230 CRC patients	109:124	230 CRC patients	109:124	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	1	71	theme	mismatch	172:179	arg1	repair					181:186	deficient DNA mismatch repair	158:186	deficient DNA mismatch repair (dMMR)	158:193	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	71	theme	mismatch	172:179	arg1	dMMR					189:192	dMMR	189:192	dMMR	189:192	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	8	72	theme	tumor	1107:1111	arg1	tissue					1113:1118	dMMR tumor tissue	1102:1118	dMMR tumor tissue	1102:1118	The richness (alpha-diversity) of gut microbiome in dMMR tumor tissue was higher compared with pMMR tumor tissues.
36312959	6	73	theme	significant	918:928	arg1	differences					940:950	the significant taxonomic differences	914:950	the significant taxonomic differences	914:950	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	13	74	theme	genetic	1951:1957	arg1	pathways					1884:1891	the biosynthesis and metabolic pathways	1853:1891	pathways	1884:1891	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	74	theme	genetic	1951:1957	arg1	replication					1959:1969	genetic replication	1951:1969	genetic replication	1951:1969	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	5	75	with	patients	776:783	arg1	pMMR					799:802	pMMR	799:802	pMMR	799:802	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	5	75	with	patients	776:783	arg1	dMMR					790:793	dMMR	790:793	dMMR	790:793	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	4	76	theme	gut	575:577	arg1	microbiota					579:588	The gut microbiota	571:588	The gut microbiota	571:588	The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing.
36312959	14	77	theme	different	2087:2095	arg1	status					2101:2106	different MMR status	2087:2106	different MMR status	2087:2106	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	7	78	theme	dMMR	993:996	arg1	status					998:1003	dMMR status	993:1003	dMMR status	993:1003	In our study cohort, dMMR status was identified in 29 of 230 (12.61%) tumors.
36312959	3	79	theme	MMR	539:541	arg1	status					543:548	the MMR status	535:548	the MMR status in 230 CRC patients	535:568	In this study, we used immunohistochemistry for four major MMR proteins to determine the MMR status in 230 CRC patients.
36312959	7	80	theme	study	979:983	arg1	cohort					985:990	our study cohort	975:990	our study cohort	975:990	In our study cohort, dMMR status was identified in 29 of 230 (12.61%) tumors.
36312959	4	81	theme	bacterial	653:661	arg1	sequencing					672:681	bacterial 16S rRNA sequencing	653:681	bacterial 16S rRNA sequencing	653:681	The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing.
36312959	9	82	theme	microbial	1169:1177	arg1	different					1259:1267	different	1259:1267	different	1259:1267	The microbial community composition (beta-diversity) between the two groups was significantly different.
36312959	9	82	theme	microbial	1169:1177	arg1	beta-diversity					1202:1215	beta-diversity	1202:1215	beta-diversity	1202:1215	The microbial community composition (beta-diversity) between the two groups was significantly different.
36312959	9	82	theme	microbial	1169:1177	arg1	composition					1189:1199	The microbial community composition	1165:1199	The microbial community composition (beta-diversity) between the two groups	1165:1239	The microbial community composition (beta-diversity) between the two groups was significantly different.
36312959	10	83	from	level	1418:1422	arg1	Bifidobacterium					1456:1470	Bifidobacterium	1456:1470	Bifidobacterium	1456:1470	The dMMR group was enriched considerably for some microbiota, including Fusobacteria, Firmicutes, Verrucomicrobia, and Actinobacteria at the phylum level and Fusobacterium, Akkermansia, Bifidobacterium, Faecalibacterium, Streptococcus, and Prevotella bacteria at the genus level.
36312959	11	84	from	level	1681:1685	arg1	Proteobacteria					1591:1604	Proteobacteria	1591:1604	Proteobacteria at the phylum level	1591:1624	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	11	84	from	level	1681:1685	arg1	Serratia					1630:1637	Serratia	1630:1637	Serratia	1630:1637	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	11	84	from	level	1681:1685	arg1	Cupriavidus					1640:1650	Cupriavidus	1640:1650	Cupriavidus	1640:1650	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	11	84	from	level	1681:1685	arg1	Sphingobium					1656:1666	Sphingobium	1656:1666	Sphingobium	1656:1666	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	4	85	theme	rRNA	667:670	arg1	sequencing					672:681	bacterial 16S rRNA sequencing	653:681	bacterial 16S rRNA sequencing	653:681	The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing.
36312959	14	86	theme	dMMR	2273:2276	arg1	tumors					2278:2283	dMMR tumors	2273:2283	dMMR tumors	2273:2283	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	5	87	theme	microbiota	703:712	arg1	diversity					714:722	microbiota diversity	703:722	microbiota diversity	703:722	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	3	88	theme	CRC	557:559	arg1	patients					561:568	230 CRC patients	553:568	230 CRC patients	553:568	In this study, we used immunohistochemistry for four major MMR proteins to determine the MMR status in 230 CRC patients.
36312959	1	89	theme	Colorectal	127:136	arg1	cancers					138:144	Colorectal cancers	127:144	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR)	127:235	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	89	theme	Colorectal	127:136	arg1	CRCs					147:150	CRCs	147:150	CRCs	147:150	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	4	90	theme	normal	629:634	arg1	tissues					636:642	cancerous and adjacent normal tissues	606:642	cancerous and adjacent normal tissues	606:642	The gut microbiota was profiled in cancerous and adjacent normal tissues by using bacterial 16S rRNA sequencing.
36312959	14	91	theme	immunotherapy	2256:2268	arg1	effectiveness					2239:2251	the effectiveness	2235:2251	the effectiveness of immunotherapy in dMMR tumors	2235:2283	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	11	92	from	level	1620:1624	arg1	Proteobacteria					1591:1604	Proteobacteria	1591:1604	Proteobacteria at the phylum level	1591:1624	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	11	92	from	level	1620:1624	arg1	Serratia					1630:1637	Serratia	1630:1637	Serratia	1630:1637	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	11	92	from	level	1620:1624	arg1	Cupriavidus					1640:1650	Cupriavidus	1640:1650	Cupriavidus	1640:1650	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	11	92	from	level	1620:1624	arg1	Sphingobium					1656:1666	Sphingobium	1656:1666	Sphingobium	1656:1666	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	1	93	theme	survival	333:340	arg1	outcomes					342:349	different survival outcomes	323:349	different survival outcomes	323:349	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	13	94	theme	KEGG	1799:1802	arg1	analysis					1812:1819	KEGG pathway analysis	1799:1819	KEGG pathway analysis	1799:1819	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	95	theme	repair	1975:1980	arg1	pathways					1982:1989	repair pathways	1975:1989	repair pathways	1975:1989	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	13	95	theme	repair	1975:1980	arg1	pathways					1884:1891	the biosynthesis and metabolic pathways	1853:1891	pathways	1884:1891	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	14	96	with	patients	2073:2080	arg1	status					2101:2106	different MMR status	2087:2106	different MMR status	2087:2106	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	13	97	from	abundance	1840:1848	arg1	samples					1999:2005	dMMR samples	1994:2005	dMMR samples	1994:2005	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	2	98	theme	gut	383:385	arg1	microbiota					387:396	gut microbiota	383:396	gut microbiota	383:396	However, it is unclear whether gut microbiota is distinct between CRCs with different MMR status.
36312959	1	99	theme	tumor	259:263	arg1	characteristics					265:279	heterogeneous tumor characteristics	245:279	heterogeneous tumor characteristics	245:279	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	6	100	theme	Linear	824:829	arg1	analysis					844:851	Linear discriminant analysis	824:851	Linear discriminant analysis effect size (LEfSe) analysis	824:880	Linear discriminant analysis effect size (LEfSe) analysis was further applied to validate the significant taxonomic differences at the genus level.
36312959	2	101	theme	MMR	438:440	arg1	status					442:447	different MMR status	428:447	different MMR status	428:447	However, it is unclear whether gut microbiota is distinct between CRCs with different MMR status.
36312959	10	102	theme	phylum	1411:1416	arg1	level					1418:1422	the phylum level	1407:1422	the phylum level	1407:1422	The dMMR group was enriched considerably for some microbiota, including Fusobacteria, Firmicutes, Verrucomicrobia, and Actinobacteria at the phylum level and Fusobacterium, Akkermansia, Bifidobacterium, Faecalibacterium, Streptococcus, and Prevotella bacteria at the genus level.
36312959	11	103	theme	phylum	1613:1618	arg1	level					1620:1624	the phylum level	1609:1624	the phylum level	1609:1624	However, the pMMR group was dominated by Proteobacteria at the phylum level and Serratia, Cupriavidus and Sphingobium at the genus level.
36312959	13	104	theme	pathways	1941:1948	arg1	abundance					1840:1848	a higher abundance	1831:1848	a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples	1831:2005	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	5	105	theme	metabolic	749:757	arg1	pathways					759:766	related metabolic pathways	741:766	related metabolic pathways	741:766	The differences in microbiota diversity, composition and related metabolic pathways between patients with dMMR and pMMR CRCs were explored.
36312959	12	106	theme	associated	1727:1736	arg1	pathways					1749:1756	microbiota associated functional pathways	1716:1756	microbiota associated functional pathways	1716:1756	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	0	107	with	microbiota	4:13	arg1	repair					90:95	deficient and proficient mismatch repair	56:95	deficient and proficient mismatch repair	56:95	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	12	108	theme	MMR	1787:1789	arg1	status					1791:1796	different MMR status	1777:1796	different MMR status	1777:1796	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	12	109	theme	pathways	1749:1756	arg1	pathways					1749:1756	microbiota associated functional pathways	1716:1756	microbiota associated functional pathways	1716:1756	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	12	109	theme	pathways	1749:1756	arg1	variety					1705:1711	a wide variety	1698:1711	a wide variety of microbiota associated functional pathways	1698:1756	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	13	110	theme	cell	1919:1922	arg1	growth					1924:1929	cell growth	1919:1929	cell growth	1919:1929	KEGG pathway analysis indicated a higher abundance of the biosynthesis and metabolic pathways of glycan and nucleotide, cell growth and death pathways, genetic replication and repair pathways in dMMR samples compared with the pMMR group.
36312959	0	111	dep	microbiota	4:13	arg1	study					100:104	A study	98:104	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.	0:125	Gut microbiota distinct between colorectal cancers with deficient and proficient mismatch repair: A study of 230 CRC patients.
36312959	12	112	theme	wide	1700:1703	arg1	pathways					1749:1756	microbiota associated functional pathways	1716:1756	microbiota associated functional pathways	1716:1756	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	12	112	theme	wide	1700:1703	arg1	variety					1705:1711	a wide variety	1698:1711	a wide variety of microbiota associated functional pathways	1698:1756	Moreover, a wide variety of microbiota associated functional pathways were observed with different MMR status.
36312959	14	113	from	effectiveness	2239:2251	arg1	tumors					2278:2283	dMMR tumors	2273:2283	dMMR tumors	2273:2283	These findings demonstrate that CRC patients with different MMR status have distinct gut bacterial community richness, compositions and related metabolic pathways, suggesting basis that may explain the effectiveness of immunotherapy in dMMR tumors.
36312959	3	114	used	used	468:471	arg2	we					465:466	we	465:466	we	465:466	In this study, we used immunohistochemistry for four major MMR proteins to determine the MMR status in 230 CRC patients.
36312959	1	115	theme	DNA	210:212	arg1	repair					223:228	proficient DNA mismatch repair	199:228	proficient DNA mismatch repair (pMMR)	199:235	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
36312959	1	115	theme	DNA	210:212	arg1	pMMR					231:234	pMMR	231:234	pMMR	231:234	Colorectal cancers (CRCs) with deficient DNA mismatch repair (dMMR) and proficient DNA mismatch repair (pMMR) exhibit heterogeneous tumor characteristics, distinct responses to immunotherapy, and different survival outcomes.
37149532	0	0	theme	Fecal	70:74	arg1	Material					76:83	Fecal Material	70:83	Fecal Material	70:83	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	4	1	theme	gel	511:513	arg1	methods					531:537	traditional gel electrophoresis methods	499:537	traditional gel electrophoresis methods	499:537	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	5	2	theme	agarose-polyacrylamide	683:704	arg1	gels					720:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	1	3	theme	critical	166:173	arg1	roles					175:179	critical roles	166:179	critical roles	166:179	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	5	4	theme	band	769:772	arg1	separation					774:783	band separation	769:783	band separation	769:783	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	3	5	from	scrapings	431:439	arg1	mucins					408:413	human and murine mucins	391:413	human and murine mucins from intestinal scrapings or fecal material	391:457	Here we describe how to extract and semi-purify human and murine mucins from intestinal scrapings or fecal material.
37149532	5	6	theme	SDS-UAgPAGE	707:717	arg1	gels					720:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	1	7	contain	have	161:164	arg2	roles					175:179	critical roles	166:179	critical roles	166:179	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	7	contain	have	161:164	arg1	glycoproteins					141:153	highly O-glycosylated polymeric glycoproteins	109:153	highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult	109:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	7	contain	have	161:164	arg1	mucins					98:103	Gel-forming mucins	86:103	Gel-forming mucins	86:103	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	5	8	theme	sodium	655:660	arg1	gels					720:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	5	9	theme	composite	645:653	arg1	gels					720:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	5	10	theme	extracted	788:796	arg1	mucins					798:803	extracted mucins	788:803	extracted mucins	788:803	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	4	11	theme	traditional	499:509	arg1	methods					531:537	traditional gel electrophoresis methods	499:537	traditional gel electrophoresis methods	499:537	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	5	12	theme	dodecyl	662:668	arg1	gels					720:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	5	13	theme	sulfate	670:676	arg1	gels					720:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	4	14	theme	molecular	480:488	arg1	weights					490:496	high molecular weights	475:496	high molecular weights	475:496	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	1	15	theme	tissue	184:189	arg1	protection					191:200	tissue protection	184:200	tissue protection from environmental insult	184:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	3	16	theme	murine	401:406	arg1	mucins					408:413	human and murine mucins	391:413	human and murine mucins from intestinal scrapings or fecal material	391:457	Here we describe how to extract and semi-purify human and murine mucins from intestinal scrapings or fecal material.
37149532	5	17	theme	urea	678:681	arg1	gels					720:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels	645:723	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	2	18	theme	biological	323:332	arg1	samples					334:340	biological samples	323:340	biological samples	323:340	To understand their biochemical properties, these samples must be extracted and enriched from biological samples.
37149532	3	19	theme	fecal	444:448	arg1	material					450:457	fecal material	444:457	fecal material	444:457	Here we describe how to extract and semi-purify human and murine mucins from intestinal scrapings or fecal material.
37149532	1	20	theme	Gel-forming	86:96	arg1	glycoproteins					141:153	highly O-glycosylated polymeric glycoproteins	109:153	highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult	109:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	20	theme	Gel-forming	86:96	arg1	mucins					98:103	Gel-forming mucins	86:103	Gel-forming mucins	86:103	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	4	21	contain	have	470:473	arg1	mucins					463:468	mucins	463:468	mucins	463:468	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	4	21	contain	have	470:473	arg2	weights					490:496	high molecular weights	475:496	high molecular weights	475:496	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	3	22	theme	human	391:395	arg1	mucins					408:413	human and murine mucins	391:413	human and murine mucins from intestinal scrapings or fecal material	391:457	Here we describe how to extract and semi-purify human and murine mucins from intestinal scrapings or fecal material.
37149532	5	23	theme	accurate	743:750	arg1	verification					752:763	accurate verification	743:763	accurate verification	743:763	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	1	24	theme	environmental	207:219	arg1	insult					221:226	environmental insult	207:226	environmental insult	207:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	3	25	theme	intestinal	420:429	arg1	scrapings					431:439	intestinal scrapings	420:439	intestinal scrapings	420:439	Here we describe how to extract and semi-purify human and murine mucins from intestinal scrapings or fecal material.
37149532	2	26	theme	biochemical	249:259	arg1	properties					261:270	their biochemical properties	243:270	their biochemical properties	243:270	To understand their biochemical properties, these samples must be extracted and enriched from biological samples.
37149532	0	27	theme	Mouse	31:35	arg1	Verification					15:26	Verification	15:26	Verification	15:26	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	0	27	theme	Mouse	31:35	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	1	28	gly	O-glycosylated	116:129	arg1	glycoproteins					141:153	highly O-glycosylated polymeric glycoproteins	109:153	highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult	109:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	28	gly	O-glycosylated	116:129	arg1	mucins					98:103	Gel-forming mucins	86:103	Gel-forming mucins	86:103	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	0	29	from	Tissue	59:64	arg1	Verification					15:26	Verification	15:26	Verification	15:26	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	0	29	from	Tissue	59:64	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	1	30	from	insult	221:226	arg1	protection					191:200	tissue protection	184:200	tissue protection from environmental insult	184:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	4	31	gly	glycoproteins	580:592	arg1	glycoproteins					580:592	these glycoproteins	574:592	these glycoproteins	574:592	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	0	32	theme	Human	41:45	arg1	Verification					15:26	Verification	15:26	Verification	15:26	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	0	32	theme	Human	41:45	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	1	33	gly	glycoproteins	141:153	arg1	glycoproteins					141:153	highly O-glycosylated polymeric glycoproteins	109:153	highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult	109:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	33	gly	glycoproteins	141:153	arg1	mucins					98:103	Gel-forming mucins	86:103	Gel-forming mucins	86:103	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	0	34	dep	Mouse	31:35	arg1	Mucins					47:52	Mucins	47:52	Mucins	47:52	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	4	35	theme	high	475:478	arg1	weights					490:496	high molecular weights	475:496	high molecular weights	475:496	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	1	36	theme	O-glycosylated	116:129	arg1	glycoproteins					141:153	highly O-glycosylated polymeric glycoproteins	109:153	highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult	109:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	36	theme	O-glycosylated	116:129	arg1	mucins					98:103	Gel-forming mucins	86:103	Gel-forming mucins	86:103	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	37	theme	polymeric	131:139	arg1	glycoproteins					141:153	highly O-glycosylated polymeric glycoproteins	109:153	highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult	109:226	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	1	37	theme	polymeric	131:139	arg1	mucins					98:103	Gel-forming mucins	86:103	Gel-forming mucins	86:103	Gel-forming mucins are highly O-glycosylated polymeric glycoproteins which have critical roles in tissue protection from environmental insult.
37149532	5	38	theme	mucins	798:803	arg1	verification					752:763	accurate verification	743:763	accurate verification	743:763	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	5	38	theme	mucins	798:803	arg1	separation					774:783	band separation	769:783	band separation	769:783	We describe the procedure for making composite sodium dodecyl sulfate urea agarose-polyacrylamide (SDS-UAgPAGE) gels, which allows for accurate verification and band separation of extracted mucins.
37149532	0	39	from	Material	76:83	arg1	Verification					15:26	Verification	15:26	Verification	15:26	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	0	39	from	Material	76:83	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material.
37149532	4	40	theme	electrophoresis	515:529	arg1	methods					531:537	traditional gel electrophoresis methods	499:537	traditional gel electrophoresis methods	499:537	As mucins have high molecular weights, traditional gel electrophoresis methods are unable to effectively separate these glycoproteins for analysis.
37149532	3	41	from	material	450:457	arg1	mucins					408:413	human and murine mucins	391:413	human and murine mucins from intestinal scrapings or fecal material	391:457	Here we describe how to extract and semi-purify human and murine mucins from intestinal scrapings or fecal material.
34927748	5	0	theme	LPS	619:621	arg1	concentrations					623:636	LPS concentrations	619:636	LPS concentrations	619:636	LPS concentrations were determined in liver tissue and bile.
34927748	16	1	theme	hepatic	2153:2159	arg1	inflammation					2161:2172	hepatic inflammation	2153:2172	hepatic inflammation in cholestasis	2153:2187	Impaired LPS removal aggravates hepatic inflammation in cholestasis.
34927748	10	2	theme	Intravital	1347:1356	arg1	imaging					1358:1364	Intravital imaging	1347:1364	Intravital imaging of LPS	1347:1371	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	4	3	theme	cell	600:603	arg1	infiltration					605:616	immune cell infiltration	593:616	immune cell infiltration	593:616	Liver injury was assessed by serum biochemistry, histology, molecular inflammation markers, and immune cell infiltration.
34927748	16	4	theme	Impaired	2121:2128	arg1	removal					2134:2140	Impaired LPS removal	2121:2140	Impaired LPS removal	2121:2140	Impaired LPS removal aggravates hepatic inflammation in cholestasis.
34927748	6	5	theme	intravital	748:757	arg1	microscopy					770:779	intravital two-photon microscopy	748:779	intravital two-photon microscopy	748:779	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	12	6	theme	Similar	1549:1555	arg1	findings					1557:1564	Similar findings	1549:1564	Similar findings with respect to hepatic LPS clearance	1549:1602	Similar findings with respect to hepatic LPS clearance were obtained in BDL and Mdr2 KO mice.
34927748	7	7	from	Changes	914:920	arg1	composition					940:950	gut microbiota composition	925:950	gut microbiota composition	925:950	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	11	8	theme	LPS	1516:1518	arg1	uptake					1520:1525	LPS uptake	1516:1525	LPS uptake into KCs	1516:1534	Moreover, LPS uptake into KCs was reduced.
34927748	14	9	theme	WT	1868:1869	arg1	mice					1883:1886	WT and Bsep KO mice	1868:1886	WT and Bsep KO mice	1868:1886	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	5	10	theme	liver	657:661	arg1	tissue					663:668	liver tissue	657:668	liver tissue	657:668	LPS concentrations were determined in liver tissue and bile.
34927748	16	11	from	inflammation	2161:2172	arg1	cholestasis					2177:2187	cholestasis	2177:2187	cholestasis	2177:2187	Impaired LPS removal aggravates hepatic inflammation in cholestasis.
34927748	14	12	theme	microbiome	1849:1858	arg1	change					1831:1836	no change	1828:1836	no change of the gut microbiome between WT and Bsep KO mice	1828:1886	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	8	13	theme	cytokines	1172:1180	arg1	production					1142:1151	production	1142:1151	production of proinflammatory cytokines	1142:1180	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	7	14	theme	gene	988:991	arg1	analysis					1013:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	1	15	theme	liver	215:219	arg1	diseases					221:228	cholestatic liver diseases	203:228	cholestatic liver diseases	203:228	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance is delayed in cholestatic liver diseases.
34927748	6	16	theme	protein	888:894	arg1	mice					908:911	common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice	832:911	common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice	832:911	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	13	17	theme	biliary	1708:1714	arg1	excretion					1716:1724	biliary excretion	1708:1724	biliary excretion of LPS in WT mice	1708:1742	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	7	18	theme	ribosomal	974:982	arg1	analysis					1013:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	7	19	theme	gut	925:927	arg1	composition					940:950	gut microbiota composition	925:950	gut microbiota composition	925:950	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	6	20	theme	multidrug	867:875	arg1	protein					888:894	multidrug resistance protein 2	867:896	multidrug resistance protein 2 (Mdr2)	867:903	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	6	20	theme	multidrug	867:875	arg1	Mdr2					899:902	Mdr2	899:902	Mdr2	899:902	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	9	21	theme	LPS	1237:1239	arg1	administration					1241:1254	LPS administration	1237:1254	LPS administration	1237:1254	After LPS administration, its concentrations were higher in liver but lower in bile of Bsep KO compared to WT mice.
34927748	2	22	theme	Kupffer	262:268	arg1	KCs					277:279	KCs	277:279	KCs	277:279	While compromised clearance by Kupffer cells (KCs) is involved, the role of LPS uptake into hepatocytes and canalicular excretion remains unclear.
34927748	2	22	theme	Kupffer	262:268	arg1	cells					270:274	Kupffer cells	262:274	Kupffer cells (KCs)	262:280	While compromised clearance by Kupffer cells (KCs) is involved, the role of LPS uptake into hepatocytes and canalicular excretion remains unclear.
34927748	1	23	theme	BACKGROUND	134:143	arg1	clearance					179:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance	134:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance	134:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance is delayed in cholestatic liver diseases.
34927748	0	24	theme	liver	68:72	arg1	injury					74:79	lipopolysaccharide-induced liver injury	41:79	lipopolysaccharide-induced liver injury in mice	41:87	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	1	25	theme	AIMS	149:152	arg1	clearance					179:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance	134:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance	134:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance is delayed in cholestatic liver diseases.
34927748	12	26	theme	KO	1634:1635	arg1	mice					1637:1640	BDL and Mdr2 KO mice	1621:1640	BDL and Mdr2 KO mice	1621:1640	Similar findings with respect to hepatic LPS clearance were obtained in BDL and Mdr2 KO mice.
34927748	13	27	with	Pretreatment	1643:1654	arg1	colchicine					1687:1696	the microtubule inhibitor colchicine	1661:1696	the microtubule inhibitor colchicine	1661:1696	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	10	28	theme	delayed	1382:1388	arg1	clearance					1390:1398	a delayed clearance	1380:1398	a delayed clearance from sinusoidal blood	1380:1420	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	15	29	from	Absence	1961:1967	arg1	general					1996:2002	general	1996:2002	general	1996:2002	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	0	30	theme	impaired	96:103	arg1	clearance					123:131	impaired hepatic endotoxin clearance	96:131	impaired hepatic endotoxin clearance	96:131	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	13	31	from	excretion	1716:1724	arg1	mice					1739:1742	WT mice	1736:1742	WT mice	1736:1742	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	3	32	dep	Wild-type	399:407	arg1	WT					410:411	WT	410:411	WT	410:411	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	14	33	theme	WT	1940:1941	arg1	mice					1943:1946	WT mice	1940:1946	WT mice	1940:1946	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	12	34	theme	LPS	1590:1592	arg1	clearance					1594:1602	hepatic LPS clearance	1582:1602	hepatic LPS clearance	1582:1602	Similar findings with respect to hepatic LPS clearance were obtained in BDL and Mdr2 KO mice.
34927748	0	35	theme	bile	8:11	arg1	pump					25:28	bile salt export pump	8:28	bile salt export pump	8:28	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	3	36	theme	Wild-type	399:407	arg1	mice					461:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	3	36	theme	Wild-type	399:407	arg1	KO					457:458	KO	457:458	KO	457:458	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	10	37	theme	uptake	1441:1446	arg1	block					1448:1452	a basolateral uptake block	1427:1452	a basolateral uptake block into hepatocytes	1427:1469	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	3	38	theme	salt	423:426	arg1	Bsep					441:444	Bsep	441:444	Bsep	441:444	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	3	38	theme	salt	423:426	arg1	pump					435:438	bile salt export pump	418:438	bile salt export pump (Bsep)	418:445	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	8	39	theme	immune	1205:1210	arg1	infiltration					1217:1228	hepatic immune cell infiltration	1197:1228	hepatic immune cell infiltration	1197:1228	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	9	40	theme	Bsep	1318:1321	arg1	KO					1323:1324	Bsep KO	1318:1324	Bsep KO	1318:1324	After LPS administration, its concentrations were higher in liver but lower in bile of Bsep KO compared to WT mice.
34927748	0	41	theme	export	18:23	arg1	pump					25:28	bile salt export pump	8:28	bile salt export pump	8:28	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	10	42	theme	canalicular	1483:1493	arg1	secretion					1495:1503	canalicular secretion	1483:1503	canalicular secretion	1483:1503	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	6	43	theme	labeled	718:724	arg1	LPS					726:728	fluorescently labeled LPS	704:728	fluorescently labeled LPS	704:728	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	3	44	theme	knockout	447:454	arg1	mice					461:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	3	44	theme	knockout	447:454	arg1	KO					457:458	KO	457:458	KO	457:458	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	8	45	theme	Bsep	1023:1026	arg1	mice					1031:1034	Bsep KO mice	1023:1034	Bsep KO mice	1023:1034	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	6	46	theme	Bsep	802:805	arg1	mice					810:813	Bsep KO mice	802:813	Bsep KO mice	802:813	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	14	47	theme	major	1904:1908	arg1	changes					1910:1916	major changes	1904:1916	major changes upon LPS challenge	1904:1935	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	2	48	theme	LPS	307:309	arg1	uptake					311:316	LPS uptake	307:316	LPS uptake	307:316	While compromised clearance by Kupffer cells (KCs) is involved, the role of LPS uptake into hepatocytes and canalicular excretion remains unclear.
34927748	9	49	theme	WT	1338:1339	arg1	mice					1341:1344	WT mice	1338:1344	WT mice	1338:1344	After LPS administration, its concentrations were higher in liver but lower in bile of Bsep KO compared to WT mice.
34927748	15	50	theme	uptake	2042:2047	arg1	block					2049:2053	a basolateral uptake block	2028:2053	a basolateral uptake block into hepatocytes	2028:2070	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	14	51	theme	Microbiota	1801:1810	arg1	analysis					1812:1819	Microbiota analysis	1801:1819	Microbiota analysis	1801:1819	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	6	52	theme	Subcellular	680:690	arg1	kinetics					692:699	Subcellular kinetics	680:699	Subcellular kinetics of fluorescently labeled LPS	680:728	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	4	53	theme	immune	593:598	arg1	infiltration					605:616	immune cell infiltration	593:616	immune cell infiltration	593:616	Liver injury was assessed by serum biochemistry, histology, molecular inflammation markers, and immune cell infiltration.
34927748	8	54	theme	LPS-induced	1062:1072	arg1	injury					1080:1085	more pronounced LPS-induced liver injury	1046:1085	more pronounced LPS-induced liver injury	1046:1085	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	14	55	theme	Bsep	1875:1878	arg1	mice					1883:1886	WT and Bsep KO mice	1868:1886	WT and Bsep KO mice	1868:1886	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	13	56	theme	WT	1736:1737	arg1	mice					1739:1742	WT mice	1736:1742	WT mice	1736:1742	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	8	57	theme	inflammatory	1091:1102	arg1	signaling					1104:1112	inflammatory signaling	1091:1112	inflammatory signaling	1091:1112	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	6	58	theme	duct-ligated	844:855	arg1	mice					908:911	common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice	832:911	common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice	832:911	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	4	59	theme	serum	526:530	arg1	biochemistry					532:543	serum biochemistry	526:543	serum biochemistry	526:543	Liver injury was assessed by serum biochemistry, histology, molecular inflammation markers, and immune cell infiltration.
34927748	6	60	theme	two-photon	759:768	arg1	microscopy					770:779	intravital two-photon microscopy	748:779	intravital two-photon microscopy	748:779	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	14	61	theme	KO	1880:1881	arg1	mice					1883:1886	WT and Bsep KO mice	1868:1886	WT and Bsep KO mice	1868:1886	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	7	62	theme	sequencing	1002:1011	arg1	analysis					1013:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	8	63	theme	hepatic	1197:1203	arg1	infiltration					1217:1228	hepatic immune cell infiltration	1197:1228	hepatic immune cell infiltration	1197:1228	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	10	64	theme	LPS	1369:1371	arg1	imaging					1358:1364	Intravital imaging	1347:1364	Intravital imaging of LPS	1347:1371	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	6	65	theme	KO	905:906	arg1	mice					908:911	common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice	832:911	common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice	832:911	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	8	66	theme	cell	1212:1215	arg1	infiltration					1217:1228	hepatic immune cell infiltration	1197:1228	hepatic immune cell infiltration	1197:1228	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	13	67	theme	LPS	1729:1731	arg1	excretion					1716:1724	biliary excretion	1708:1724	biliary excretion of LPS in WT mice	1708:1742	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	7	68	theme	amplicon	993:1000	arg1	analysis					1013:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	4	69	theme	inflammation	567:578	arg1	markers					580:586	molecular inflammation markers	557:586	molecular inflammation markers	557:586	Liver injury was assessed by serum biochemistry, histology, molecular inflammation markers, and immune cell infiltration.
34927748	6	70	from	findings	790:797	arg1	mice					810:813	Bsep KO mice	802:813	Bsep KO mice	802:813	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	7	71	theme	RNA	984:986	arg1	analysis					1013:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	2	72	theme	compromised	237:247	arg1	clearance					249:257	compromised clearance	237:257	compromised clearance by Kupffer cells (KCs)	237:280	While compromised clearance by Kupffer cells (KCs) is involved, the role of LPS uptake into hepatocytes and canalicular excretion remains unclear.
34927748	1	73	theme	cholestatic	203:213	arg1	diseases					221:228	cholestatic liver diseases	203:228	cholestatic liver diseases	203:228	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance is delayed in cholestatic liver diseases.
34927748	8	74	theme	proinflammatory	1156:1170	arg1	cytokines					1172:1180	proinflammatory cytokines	1156:1180	proinflammatory cytokines	1156:1180	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	10	75	from	blood	1416:1420	arg1	clearance					1390:1398	a delayed clearance	1380:1398	a delayed clearance from sinusoidal blood	1380:1420	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	0	76	from	injury	74:79	arg1	mice					84:87	mice	84:87	mice	84:87	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	16	77	theme	LPS	2130:2132	arg1	removal					2134:2140	Impaired LPS removal	2121:2140	Impaired LPS removal	2121:2140	Impaired LPS removal aggravates hepatic inflammation in cholestasis.
34927748	7	78	theme	16S	970:972	arg1	analysis					1013:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	16S ribosomal RNA gene amplicon sequencing analysis	970:1020	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	6	79	theme	resistance	877:886	arg1	protein					888:894	multidrug resistance protein 2	867:896	multidrug resistance protein 2 (Mdr2)	867:903	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	6	79	theme	resistance	877:886	arg1	Mdr2					899:902	Mdr2	899:902	Mdr2	899:902	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	15	80	theme	basolateral	2030:2040	arg1	block					2049:2053	a basolateral uptake block	2028:2053	a basolateral uptake block into hepatocytes	2028:2070	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	7	81	theme	microbiota	929:938	arg1	composition					940:950	gut microbiota composition	925:950	gut microbiota composition	925:950	Changes in gut microbiota composition were evaluated by 16S ribosomal RNA gene amplicon sequencing analysis.
34927748	1	82	theme	Lipopolysaccharide	154:171	arg1	clearance					179:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance	134:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance	134:187	BACKGROUND AND AIMS Lipopolysaccharide (LPS) clearance is delayed in cholestatic liver diseases.
34927748	12	83	theme	Mdr2	1629:1632	arg1	mice					1637:1640	BDL and Mdr2 KO mice	1621:1640	BDL and Mdr2 KO mice	1621:1640	Similar findings with respect to hepatic LPS clearance were obtained in BDL and Mdr2 KO mice.
34927748	0	84	theme	endotoxin	113:121	arg1	clearance					123:131	impaired hepatic endotoxin clearance	96:131	impaired hepatic endotoxin clearance	96:131	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	15	85	theme	LPS	2011:2013	arg1	clearance					2015:2023	LPS clearance	2011:2023	LPS clearance by a basolateral uptake block into hepatocytes	2011:2070	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	13	86	theme	microtubule	1665:1675	arg1	colchicine					1687:1696	the microtubule inhibitor colchicine	1661:1696	the microtubule inhibitor colchicine	1661:1696	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	0	87	theme	hepatic	105:111	arg1	clearance					123:131	impaired hepatic endotoxin clearance	96:131	impaired hepatic endotoxin clearance	96:131	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	13	88	theme	inhibitor	1677:1685	arg1	colchicine					1687:1696	the microtubule inhibitor colchicine	1661:1696	the microtubule inhibitor colchicine	1661:1696	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	3	89	theme	bile	418:421	arg1	Bsep					441:444	Bsep	441:444	Bsep	441:444	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	3	89	theme	bile	418:421	arg1	pump					435:438	bile salt export pump	418:438	bile salt export pump (Bsep)	418:445	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	15	90	theme	cholestasis	1981:1991	arg1	Absence					1961:1967	CONCLUSIONS Absence	1949:1967	CONCLUSIONS Absence of Bsep and cholestasis in general	1949:2002	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	12	91	theme	BDL	1621:1623	arg1	mice					1637:1640	BDL and Mdr2 KO mice	1621:1640	BDL and Mdr2 KO mice	1621:1640	Similar findings with respect to hepatic LPS clearance were obtained in BDL and Mdr2 KO mice.
34927748	3	92	theme	APPROACH	378:385	arg1	mice					461:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	3	92	theme	APPROACH	378:385	arg1	KO					457:458	KO	457:458	KO	457:458	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	4	93	theme	Liver	497:501	arg1	injury					503:508	Liver injury	497:508	Liver injury	497:508	Liver injury was assessed by serum biochemistry, histology, molecular inflammation markers, and immune cell infiltration.
34927748	10	94	theme	sinusoidal	1405:1414	arg1	blood					1416:1420	sinusoidal blood	1405:1420	sinusoidal blood	1405:1420	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	0	95	theme	salt	13:16	arg1	pump					25:28	bile salt export pump	8:28	bile salt export pump	8:28	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	15	96	theme	Bsep	1972:1975	arg1	Absence					1961:1967	CONCLUSIONS Absence	1949:1967	CONCLUSIONS Absence of Bsep and cholestasis in general	1949:2002	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	12	97	theme	hepatic	1582:1588	arg1	clearance					1594:1602	hepatic LPS clearance	1582:1602	hepatic LPS clearance	1582:1602	Similar findings with respect to hepatic LPS clearance were obtained in BDL and Mdr2 KO mice.
34927748	0	98	theme	pump	25:28	arg1	Loss					0:3	Loss	0:3	Loss of bile salt export pump	0:28	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	8	99	theme	KO	1028:1029	arg1	mice					1031:1034	Bsep KO mice	1023:1034	Bsep KO mice	1023:1034	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	14	100	theme	gut	1845:1847	arg1	microbiome					1849:1858	the gut microbiome	1841:1858	the gut microbiome	1841:1858	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	14	101	theme	LPS	1923:1925	arg1	challenge					1927:1935	LPS challenge	1923:1935	LPS challenge	1923:1935	Microbiota analysis showed no change of the gut microbiome between WT and Bsep KO mice at baseline but major changes upon LPS challenge in WT mice.
34927748	10	102	theme	basolateral	1429:1439	arg1	block					1448:1452	a basolateral uptake block	1427:1452	a basolateral uptake block into hepatocytes	1427:1469	Intravital imaging of LPS showed a delayed clearance from sinusoidal blood with a basolateral uptake block into hepatocytes and reduced canalicular secretion.
34927748	6	103	theme	LPS	726:728	arg1	kinetics					692:699	Subcellular kinetics	680:699	Subcellular kinetics of fluorescently labeled LPS	680:728	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	3	104	theme	export	428:433	arg1	Bsep					441:444	Bsep	441:444	Bsep	441:444	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	3	104	theme	export	428:433	arg1	pump					435:438	bile salt export pump	418:438	bile salt export pump (Bsep)	418:445	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	0	105	theme	lipopolysaccharide-induced	41:66	arg1	injury					74:79	lipopolysaccharide-induced liver injury	41:79	lipopolysaccharide-induced liver injury in mice	41:87	Loss of bile salt export pump aggravates lipopolysaccharide-induced liver injury in mice due to impaired hepatic endotoxin clearance.
34927748	9	106	theme	KO	1323:1324	arg1	bile					1310:1313	bile	1310:1313	bile of Bsep KO	1310:1324	After LPS administration, its concentrations were higher in liver but lower in bile of Bsep KO compared to WT mice.
34927748	6	107	theme	KO	807:808	arg1	mice					810:813	Bsep KO mice	802:813	Bsep KO mice	802:813	Subcellular kinetics of fluorescently labeled LPS was visualized by intravital two-photon microscopy, and the findings in Bsep KO mice were compared to common bile duct-ligated (BDL) and multidrug resistance protein 2 (Mdr2) KO mice.
34927748	3	108	theme	RESULTS	391:397	arg1	mice					461:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice	378:464	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	3	108	theme	RESULTS	391:397	arg1	KO					457:458	KO	457:458	KO	457:458	APPROACH AND RESULTS Wild-type (WT) and bile salt export pump (Bsep) knockout (KO) mice were challenged i.p. with LPS.
34927748	2	109	theme	uptake	311:316	arg1	role					299:302	the role	295:302	the role of LPS uptake into hepatocytes and canalicular excretion	295:359	While compromised clearance by Kupffer cells (KCs) is involved, the role of LPS uptake into hepatocytes and canalicular excretion remains unclear.
34927748	8	110	theme	pronounced	1051:1060	arg1	injury					1080:1085	more pronounced LPS-induced liver injury	1046:1085	more pronounced LPS-induced liver injury	1046:1085	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	15	111	theme	CONCLUSIONS	1949:1959	arg1	Absence					1961:1967	CONCLUSIONS Absence	1949:1967	CONCLUSIONS Absence of Bsep and cholestasis in general	1949:2002	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	8	112	theme	liver	1074:1078	arg1	injury					1080:1085	more pronounced LPS-induced liver injury	1046:1085	more pronounced LPS-induced liver injury	1046:1085	Bsep KO mice developed more pronounced LPS-induced liver injury and inflammatory signaling, with subsequently enhanced production of proinflammatory cytokines and aggravated hepatic immune cell infiltration.
34927748	15	113	theme	less	2089:2092	arg1	secretion					2094:2102	consequently less secretion	2076:2102	consequently less secretion into canaliculi	2076:2118	CONCLUSIONS Absence of Bsep and cholestasis in general impair LPS clearance by a basolateral uptake block into hepatocytes and consequently less secretion into canaliculi.
34927748	4	114	theme	molecular	557:565	arg1	markers					580:586	molecular inflammation markers	557:586	molecular inflammation markers	557:586	Liver injury was assessed by serum biochemistry, histology, molecular inflammation markers, and immune cell infiltration.
34927748	13	115	theme	LPS	1761:1763	arg1	clearance					1765:1773	LPS clearance	1761:1773	LPS clearance	1761:1773	Pretreatment with the microtubule inhibitor colchicine inhibited biliary excretion of LPS in WT mice, indicating that LPS clearance is microtubule-dependent.
34927748	2	116	theme	canalicular	339:349	arg1	excretion					351:359	canalicular excretion	339:359	canalicular excretion	339:359	While compromised clearance by Kupffer cells (KCs) is involved, the role of LPS uptake into hepatocytes and canalicular excretion remains unclear.
35099962	0	0	theme	Sorghum	64:70	arg1	Stalks					72:77	Its Mutant Sweet Sorghum Stalks	47:77	Its Mutant Sweet Sorghum Stalks	47:77	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	6	1	theme	Structural	950:959	arg1	variations					961:970	Structural variations	950:970	Structural variations due to changes in both cellulose- and hemicellulose-based sugars	950:1035	Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected.
35099962	6	2	theme	cellulose-	995:1004	arg1	sugars					1030:1035	both cellulose- and hemicellulose-based sugars	990:1035	both cellulose- and hemicellulose-based sugars	990:1035	Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected.
35099962	1	3	dep	internodes	240:249	arg1	IN					252:253	IN	252:253	IN	252:253	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	4	theme	variant	197:203	arg1	REDforGREEN					205:215	its variant REDforGREEN	193:215	its variant REDforGREEN (RG)	193:220	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	4	theme	variant	197:203	arg1	RG					218:219	RG	218:219	RG	218:219	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	0	5	theme	Sweet	58:62	arg1	Stalks					72:77	Its Mutant Sweet Sorghum Stalks	47:77	Its Mutant Sweet Sorghum Stalks	47:77	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	5	6	theme	glucan	868:873	arg1	content					875:881	The glucan content	864:881	The glucan content	864:881	The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes.
35099962	4	7	from	stalks	695:700	arg1	lignin					671:676	lignin	671:676	lignin from the sorghum stalks	671:700	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	7	8	located	detected	1131:1138	arg2	linkages					1122:1129	the main interunit linkages	1103:1129	the main interunit linkages detected in lignin	1103:1148	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	7	8	located	detected	1131:1138	arg1	lignin					1143:1148	lignin	1143:1148	lignin	1143:1148	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	7	8	located	detected	1131:1138	arg2	linkages					1089:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	4	9	theme	syringyl/guaiacyl	741:757	arg1	ratio					759:763	the syringyl/guaiacyl ratio	737:763	the syringyl/guaiacyl ratio	737:763	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	8	10	theme	RG	1219:1220	arg1	variation					1222:1230	the RG variation	1215:1230	the RG variation	1215:1230	These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.
35099962	0	11	theme	Stalks	72:77	arg1	Chemistry					24:32	Cell Wall Chemistry	14:32	Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks	14:77	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	1	12	theme	1H-13C	431:436	arg1	NMR					466:468	NMR	466:468	NMR	466:468	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	12	theme	1H-13C	431:436	arg1	resonance					455:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance	410:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR)	410:469	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	3	13	theme	Klason	554:559	arg1	reductions					568:577	Klason lignin reductions	554:577	Klason lignin reductions by 16-44 and 2-26%	554:596	In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively.
35099962	1	14	theme	nuclear	438:444	arg1	NMR					466:468	NMR	466:468	NMR	466:468	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	14	theme	nuclear	438:444	arg1	resonance					455:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance	410:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR)	410:469	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	6	15	from	changes	979:985	arg1	sugars					1030:1035	both cellulose- and hemicellulose-based sugars	990:1035	both cellulose- and hemicellulose-based sugars	990:1035	Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected.
35099962	1	16	theme	magnetic	446:453	arg1	NMR					466:468	NMR	466:468	NMR	466:468	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	16	theme	magnetic	446:453	arg1	resonance					455:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance	410:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR)	410:469	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	7	17	theme	nonacylated	1056:1066	arg1	linkages					1122:1129	the main interunit linkages	1103:1129	the main interunit linkages detected in lignin	1103:1148	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	7	17	theme	nonacylated	1056:1066	arg1	linkages					1089:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	2	18	dep	2018	497:500	arg1	RG1					510:512	RG1	510:512	RG1	510:512	The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons.
35099962	2	18	dep	2018	497:500	arg1	D1					503:504	D1	503:504	D1	503:504	The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons.
35099962	1	19	theme	high-performance	276:291	arg1	HPLC					316:319	HPLC	316:319	HPLC	316:319	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	19	theme	high-performance	276:291	arg1	chromatography					300:313	high-performance liquid chromatography	276:313	high-performance liquid chromatography (HPLC)	276:320	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	2	20	theme	2018	497:500	arg1	seasons					537:543	2018 (D1 and RG1) and 2019 (D2 and RG2) seasons	497:543	2018 (D1 and RG1) and 2019 (D2 and RG2) seasons	497:543	The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons.
35099962	1	21	theme	X-ray	381:385	arg1	XRD					400:402	XRD	400:402	XRD	400:402	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	21	theme	X-ray	381:385	arg1	diffraction					387:397	X-ray diffraction	381:397	X-ray diffraction (XRD)	381:403	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	8	22	theme	growing	1241:1247	arg1	season					1249:1254	the growing season	1237:1254	the growing season	1237:1254	These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.
35099962	1	23	theme	sweet	154:158	arg1	varieties					168:176	two sweet sorghum varieties	150:176	two sweet sorghum varieties	150:176	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	3	24	located	detected	603:610	arg1	IN					615:616	IN	615:616	IN	615:616	In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively.
35099962	3	24	located	detected	603:610	arg1	RG1					549:551	RG1	549:551	RG1	549:551	In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively.
35099962	3	24	located	detected	603:610	arg2	reductions					568:577	Klason lignin reductions	554:577	Klason lignin reductions by 16-44 and 2-26%	554:596	In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively.
35099962	3	24	located	detected	603:610	arg1	N					622:622	N	622:622	N	622:622	In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively.
35099962	1	25	dep	variation	137:145	arg1	polysaccharides					120:134	polysaccharides	120:134	polysaccharides	120:134	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	25	dep	variation	137:145	arg1	lignin					109:114	lignin	109:114	lignin	109:114	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	26	theme	sorghum	160:166	arg1	varieties					168:176	two sweet sorghum varieties	150:176	two sweet sorghum varieties	150:176	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	0	27	theme	Wall	19:22	arg1	Chemistry					24:32	Cell Wall Chemistry	14:32	Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks	14:77	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	4	28	theme	guaiacyl	718:725	arg1	units					727:731	guaiacyl units	718:731	guaiacyl units	718:731	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	2	29	theme	2019	519:522	arg1	seasons					537:543	2018 (D1 and RG1) and 2019 (D2 and RG2) seasons	497:543	2018 (D1 and RG1) and 2019 (D2 and RG2) seasons	497:543	The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons.
35099962	1	30	theme	varieties	168:176	arg1	REDforGREEN					205:215	its variant REDforGREEN	193:215	its variant REDforGREEN (RG)	193:220	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	30	theme	varieties	168:176	arg1	RG					218:219	RG	218:219	RG	218:219	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	30	theme	varieties	168:176	arg1	variation					137:145	The cell wall compositional (lignin and polysaccharides) variation	80:145	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties	80:176	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	0	31	theme	Cell	14:17	arg1	Chemistry					24:32	Cell Wall Chemistry	14:32	Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks	14:77	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	3	32	theme	lignin	561:566	arg1	reductions					568:577	Klason lignin reductions	554:577	Klason lignin reductions by 16-44 and 2-26%	554:596	In RG1, Klason lignin reductions by 16-44 and 2-26% were detected in IN and N, respectively.
35099962	1	33	theme	liquid	293:298	arg1	HPLC					316:319	HPLC	316:319	HPLC	316:319	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	33	theme	liquid	293:298	arg1	chromatography					300:313	high-performance liquid chromatography	276:313	high-performance liquid chromatography (HPLC)	276:320	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	34	theme	cell	84:87	arg1	variation					137:145	The cell wall compositional (lignin and polysaccharides) variation	80:145	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties	80:176	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	8	35	theme	lodging	1293:1299	arg1	behavior					1301:1308	their mechanical and lodging behavior	1272:1308	behavior	1301:1308	These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.
35099962	8	36	theme	compositional	1174:1186	arg1	variation					1188:1196	compositional variation	1174:1196	compositional variation of stalks due to the RG variation	1174:1230	These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.
35099962	7	37	theme	main	1107:1110	arg1	linkages					1122:1129	the main interunit linkages	1103:1129	the main interunit linkages detected in lignin	1103:1148	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	7	37	theme	main	1107:1110	arg1	linkages					1089:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	0	38	theme	Chemistry	24:32	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.	0:78	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	8	39	theme	stalks	1201:1206	arg1	variation					1188:1196	compositional variation	1174:1196	compositional variation of stalks due to the RG variation	1174:1230	These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.
35099962	7	40	theme	interunit	1112:1120	arg1	linkages					1122:1129	the main interunit linkages	1103:1129	the main interunit linkages detected in lignin	1103:1148	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	7	40	theme	interunit	1112:1120	arg1	linkages					1089:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	5	41	from	internodes	938:947	arg1	at					935:936	RG2 at internodes	931:947	RG2 at internodes	931:947	The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes.
35099962	5	41	from	internodes	938:947	arg1	RG2					931:933	RG2 at internodes	931:947	RG2 at internodes	931:947	The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes.
35099962	1	42	theme	wall	89:92	arg1	variation					137:145	The cell wall compositional (lignin and polysaccharides) variation	80:145	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties	80:176	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	0	43	theme	Della	37:41	arg1	Chemistry					24:32	Cell Wall Chemistry	14:32	Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks	14:77	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	4	44	theme	sorghum	687:693	arg1	stalks					695:700	the sorghum stalks	683:700	the sorghum stalks	683:700	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	1	45	theme	compositional	94:106	arg1	variation					137:145	The cell wall compositional (lignin and polysaccharides) variation	80:145	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties	80:176	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	6	46	theme	hemicellulose-based	1010:1028	arg1	sugars					1030:1035	both cellulose- and hemicellulose-based sugars	990:1035	both cellulose- and hemicellulose-based sugars	990:1035	Structural variations due to changes in both cellulose- and hemicellulose-based sugars were detected.
35099962	8	47	theme	due	1208:1210	arg1	stalks					1201:1206	stalks	1201:1206	stalks due to the RG variation	1201:1230	These results indicate compositional variation of stalks due to the RG variation, and the growing season could influence their mechanical and lodging behavior.
35099962	7	48	theme	β-O-4	1083:1087	arg1	linkages					1122:1129	the main interunit linkages	1103:1129	the main interunit linkages detected in lignin	1103:1148	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	7	48	theme	β-O-4	1083:1087	arg1	linkages					1089:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	2	49	dep	2019	519:522	arg1	RG2					532:534	RG2	532:534	RG2	532:534	The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons.
35099962	2	49	dep	2019	519:522	arg1	D2					525:526	D2	525:526	D2	525:526	The stalks were grown in 2018 (D1 and RG1) and 2019 (D2 and RG2) seasons.
35099962	5	50	theme	RG2	931:933	arg1	internodes					938:947	RG2 at internodes	931:947	RG2 at internodes	931:947	The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes.
35099962	1	51	theme	pyrolysis-gas	323:335	arg1	Py-GCMS					371:377	Py-GCMS	371:377	Py-GCMS	371:377	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	51	theme	pyrolysis-gas	323:335	arg1	spectrometry					357:368	pyrolysis-gas chromatography-mass spectrometry	323:368	pyrolysis-gas chromatography-mass spectrometry (Py-GCMS)	323:378	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	52	theme	two-dimensional	410:424	arg1	NMR					466:468	NMR	466:468	NMR	466:468	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	52	theme	two-dimensional	410:424	arg1	resonance					455:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance	410:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR)	410:469	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	4	53	from	IN	840:841	arg1	%					814:814	96%	812:814	96%	812:814	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	4	53	from	IN	840:841	arg1	%					856:856	61 and 23%	847:856	61 and 23% at N	847:861	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	5	54	theme	at	935:936	arg1	internodes					938:947	RG2 at internodes	931:947	RG2 at internodes	931:947	The glucan content was reduced by 23-27% for RG1 and by 17-22% for RG2 at internodes.
35099962	0	55	theme	Mutant	51:56	arg1	Stalks					72:77	Its Mutant Sweet Sorghum Stalks	47:77	Its Mutant Sweet Sorghum Stalks	47:77	Evaluation of Cell Wall Chemistry of Della and Its Mutant Sweet Sorghum Stalks.
35099962	1	56	theme	chromatography-mass	337:355	arg1	Py-GCMS					371:377	Py-GCMS	371:377	Py-GCMS	371:377	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	56	theme	chromatography-mass	337:355	arg1	spectrometry					357:368	pyrolysis-gas chromatography-mass spectrometry	323:368	pyrolysis-gas chromatography-mass spectrometry (Py-GCMS)	323:378	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	4	57	from	N	861:861	arg1	%					814:814	96%	812:814	96%	812:814	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	4	57	from	N	861:861	arg1	%					856:856	61 and 23%	847:856	61 and 23% at N	847:861	The analyses also revealed that lignin from the sorghum stalks was enriched in guaiacyl units and the syringyl/guaiacyl ratio was increased in RG1 and RG2, respectively, by 96% and more than 2-fold at IN and 61 and 23% at N.
35099962	7	58	theme	γ-acylated	1072:1081	arg1	linkages					1122:1129	the main interunit linkages	1103:1129	the main interunit linkages detected in lignin	1103:1148	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	7	58	theme	γ-acylated	1072:1081	arg1	linkages					1089:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages	1052:1096	The nonacylated and γ-acylated β-O-4 linkages were the main interunit linkages detected in lignin.
35099962	1	59	theme	2D	427:428	arg1	NMR					466:468	NMR	466:468	NMR	466:468	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
35099962	1	59	theme	2D	427:428	arg1	resonance					455:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance	410:463	two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR)	410:469	The cell wall compositional (lignin and polysaccharides) variation of two sweet sorghum varieties, Della (D) and its variant REDforGREEN (RG), was evaluated at internodes (IN) and nodes (N) using high-performance liquid chromatography (HPLC), pyrolysis-gas chromatography-mass spectrometry (Py-GCMS), X-ray diffraction (XRD), and two-dimensional (2D) 1H-13C nuclear magnetic resonance (NMR).
37148836	4	0	theme	fewer	674:678	arg1	complications					690:702	fewer pulmonary complications	674:702	fewer pulmonary complications	674:702	Secondarily, we tested the hypothesis that patients given sugammadex have fewer pulmonary complications during hospitalization.
37148836	13	1	theme	patients	1480:1487	arg1	patients					1480:1487	patients	1480:1487	patients given sugammadex	1480:1504	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	1	theme	patients	1480:1487	arg1	%					1475:1475	4.4%	1472:1475	4.4% of patients given sugammadex	1472:1504	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	1	theme	patients	1480:1487	arg1	patients					1518:1525	patients	1518:1525	patients given neostigmine	1518:1543	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	1	theme	patients	1480:1487	arg1	%					1513:1513	3.6%	1510:1513	3.6% of patients given neostigmine	1510:1543	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	14	2	theme	minimum	1789:1795	arg1	ratio					1807:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio	1763:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission	1763:1833	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission was similar after reversal of neuromuscular block by sugammadex and neostigmine.
37148836	3	3	dep	hypothesis	467:476	arg1	oxygenate					529:537	oxygenate	529:537	oxygenate better during initial recovery than those given neostigmine	529:597	We therefore tested the primary hypothesis that noncardiac surgical patients given sugammadex oxygenate better during initial recovery than those given neostigmine.
37148836	1	4	theme	OBJECTIVE	190:198	arg1	block					223:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	1	5	theme	other	300:304	arg1	complications					316:328	other pulmonary complications	300:328	other pulmonary complications	300:328	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	9	6	theme	lowest	992:997	arg1	ratio					1009:1013	the lowest SpO2/FiO2 ratio	988:1013	the lowest SpO2/FiO2 ratio	988:1013	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	9	6	theme	lowest	992:997	arg1	MEASUREMENTS					951:962	MEASUREMENTS The primary outcome	951:982	MEASUREMENTS The primary outcome	951:982	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	1	7	theme	neuromuscular	209:221	arg1	block					223:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	14	8	theme	Postoperative	1775:1787	arg1	ratio					1807:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio	1763:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission	1763:1833	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission was similar after reversal of neuromuscular block by sugammadex and neostigmine.
37148836	13	9	contain	had	1545:1547	arg1	%					1475:1475	4.4%	1472:1475	4.4% of patients given sugammadex	1472:1504	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	9	contain	had	1545:1547	arg2	P = 0.0005					1588:1597	P = 0.0005	1588:1597	P = 0.0005	1588:1597	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	9	contain	had	1545:1547	arg1	patients					1480:1487	patients	1480:1487	patients given sugammadex	1480:1504	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	9	contain	had	1545:1547	arg1	patients					1518:1525	patients	1518:1525	patients given neostigmine	1518:1543	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	9	contain	had	1545:1547	arg2	complications					1573:1585	postoperative pulmonary complications	1549:1585	postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330)	1549:1649	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	9	contain	had	1545:1547	arg1	%					1513:1513	3.6%	1510:1513	3.6% of patients given neostigmine	1510:1543	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	7	10	contain	had	851:853	arg1	neostigmine					904:914	neostigmine	904:914	neostigmine	904:914	PATIENTS Adults who had non-cardiothoracic surgery and were given either neostigmine or sugammadex.
37148836	7	10	contain	had	851:853	arg2	surgery					874:880	non-cardiothoracic surgery	855:880	non-cardiothoracic surgery	855:880	PATIENTS Adults who had non-cardiothoracic surgery and were given either neostigmine or sugammadex.
37148836	7	10	contain	had	851:853	arg1	Adults					840:845	PATIENTS Adults	831:845	PATIENTS Adults who had non-cardiothoracic surgery	831:880	PATIENTS Adults who had non-cardiothoracic surgery and were given either neostigmine or sugammadex.
37148836	15	11	theme	pulmonary	1966:1974	arg1	complications					1976:1988	more pulmonary complications	1961:1988	more pulmonary complications	1961:1988	Reversal with sugammadex was associated with more pulmonary complications, but most were minor and of little consequence.
37148836	0	12	theme	retrospective	156:168	arg1	study					177:181	A retrospective cohort study	154:181	A retrospective cohort study	154:181	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	12	13	theme	-3.5	1416:1419	arg1	means					1407:1411	means	1407:1411	means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002)	1407:1469	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	7	14	theme	PATIENTS	831:838	arg1	Adults					840:845	PATIENTS Adults	831:845	PATIENTS Adults who had non-cardiothoracic surgery	831:880	PATIENTS Adults who had non-cardiothoracic surgery and were given either neostigmine or sugammadex.
37148836	13	15	theme	number-needed-to-be-exposed	1600:1626	arg1	=136					1628:1631	number-needed-to-be-exposed =136	1600:1631	number-needed-to-be-exposed =136	1600:1631	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	16	theme	disease	1754:1760	arg1	exacerbation					1716:1727	exacerbation	1716:1727	exacerbation of obstructive pulmonary disease	1716:1760	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	16	theme	disease	1754:1760	arg1	components					1679:1688	the main contributing components	1657:1688	the main contributing components	1657:1688	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	16	theme	disease	1754:1760	arg1	bronchospasm					1700:1711	new bronchospasm	1696:1711	new bronchospasm	1696:1711	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	0	17	theme	neuromuscular	102:114	arg1	block					116:120	neuromuscular block	102:120	neuromuscular block	102:120	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	15	18	theme	little	2018:2023	arg1	consequence					2025:2035	little consequence	2018:2035	little consequence	2018:2035	Reversal with sugammadex was associated with more pulmonary complications, but most were minor and of little consequence.
37148836	6	19	theme	hospital	821:828	arg1	area					797:800	Postoperative recovery area	774:800	Postoperative recovery area of a tertiary care hospital	774:828	SETTING Postoperative recovery area of a tertiary care hospital.
37148836	3	20	theme	surgical	494:501	arg1	patients					503:510	noncardiac surgical patients	483:510	noncardiac surgical patients given sugammadex	483:527	We therefore tested the primary hypothesis that noncardiac surgical patients given sugammadex oxygenate better during initial recovery than those given neostigmine.
37148836	12	21	theme	estimated	1383:1391	arg1	difference					1393:1402	an estimated difference	1380:1402	an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002)	1380:1469	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	13	22	theme	obstructive	1732:1742	arg1	disease					1754:1760	obstructive pulmonary disease	1732:1760	obstructive pulmonary disease	1732:1760	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	6	23	theme	tertiary	807:814	arg1	hospital					821:828	a tertiary care hospital	805:828	a tertiary care hospital	805:828	SETTING Postoperative recovery area of a tertiary care hospital.
37148836	13	24	theme	pulmonary	1563:1571	arg1	P = 0.0005					1588:1597	P = 0.0005	1588:1597	P = 0.0005	1588:1597	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	24	theme	pulmonary	1563:1571	arg1	complications					1573:1585	postoperative pulmonary complications	1549:1585	postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330)	1549:1649	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	0	25	theme	Postoperative	0:12	arg1	oxygenation					14:24	Postoperative oxygenation	0:24	Postoperative oxygenation	0:24	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	11	26	theme	MAIN	1115:1118	arg1	RESULTS					1120:1126	MAIN RESULTS	1115:1126	MAIN RESULTS Among 71,457 cases, 10,708 (15%)	1115:1159	MAIN RESULTS Among 71,457 cases, 10,708 (15%) were given sugammadex and 60,749 (85%) received neostigmine.
37148836	9	27	theme	SpO2/FiO2	999:1007	arg1	ratio					1009:1013	the lowest SpO2/FiO2 ratio	988:1013	the lowest SpO2/FiO2 ratio	988:1013	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	9	27	theme	SpO2/FiO2	999:1007	arg1	MEASUREMENTS					951:962	MEASUREMENTS The primary outcome	951:982	MEASUREMENTS The primary outcome	951:982	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	12	28	theme	mean	1254:1257	arg1	301 ± 77					1287:1294	301 ± 77	1287:1294	301 ± 77	1287:1294	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	12	28	theme	mean	1254:1257	arg1	ratio					1277:1281	the mean minimum SpO2/FiO2 ratio	1250:1281	the mean minimum SpO2/FiO2 ratio	1250:1281	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	3	29	theme	primary	459:465	arg1	hypothesis					467:476	the primary hypothesis	455:476	the primary hypothesis that noncardiac surgical patients given sugammadex oxygenate better during initial recovery than those given neostigmine	455:597	We therefore tested the primary hypothesis that noncardiac surgical patients given sugammadex oxygenate better during initial recovery than those given neostigmine.
37148836	6	30	theme	recovery	788:795	arg1	area					797:800	Postoperative recovery area	774:800	Postoperative recovery area of a tertiary care hospital	774:828	SETTING Postoperative recovery area of a tertiary care hospital.
37148836	0	31	theme	SpO2/FiO2	38:46	arg1	ratio					48:52	SpO2/FiO2 ratio	38:52	SpO2/FiO2 ratio	38:52	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	14	32	theme	neuromuscular	1865:1877	arg1	block					1879:1883	neuromuscular block	1865:1883	neuromuscular block	1865:1883	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission was similar after reversal of neuromuscular block by sugammadex and neostigmine.
37148836	13	33	dep	P = 0.0005	1588:1597	arg1	=136					1628:1631	number-needed-to-be-exposed =136	1600:1631	number-needed-to-be-exposed =136	1600:1631	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	33	dep	P = 0.0005	1588:1597	arg1	%					1636:1636	95% CI: 83, 330	1634:1648	%	1636:1636	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	9	34	theme	post-anesthesia	1022:1036	arg1	unit					1043:1046	the post-anesthesia care unit	1018:1046	the post-anesthesia care unit	1018:1046	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	12	35	theme	propensity	1228:1237	arg1	weighting					1239:1247	propensity weighting	1228:1247	propensity weighting	1228:1247	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	10	36	theme	complications	1100:1112	arg1	outcome					1063:1069	The secondary outcome	1049:1069	The secondary outcome	1049:1069	The secondary outcome was a composite of pulmonary complications.
37148836	10	36	theme	complications	1100:1112	arg1	composite					1077:1085	a composite	1075:1085	a composite of pulmonary complications	1075:1112	The secondary outcome was a composite of pulmonary complications.
37148836	10	36	theme	complications	1100:1112	arg1	complications					1100:1112	pulmonary complications	1090:1112	pulmonary complications	1090:1112	The secondary outcome was a composite of pulmonary complications.
37148836	13	37	theme	contributing	1666:1677	arg1	components					1679:1688	the main contributing components	1657:1688	the main contributing components	1657:1688	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	37	theme	contributing	1666:1677	arg1	bronchospasm					1700:1711	new bronchospasm	1696:1711	new bronchospasm	1696:1711	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	1	38	theme	postoperative	241:253	arg1	weakness					262:269	postoperative muscle weakness	241:269	postoperative muscle weakness	241:269	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	0	39	dep	assessed	26:33	arg1	study					177:181	A retrospective cohort study	154:181	A retrospective cohort study	154:181	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	4	40	theme	pulmonary	680:688	arg1	complications					690:702	fewer pulmonary complications	674:702	fewer pulmonary complications	674:702	Secondarily, we tested the hypothesis that patients given sugammadex have fewer pulmonary complications during hospitalization.
37148836	7	41	theme	non-cardiothoracic	855:872	arg1	surgery					874:880	non-cardiothoracic surgery	855:880	non-cardiothoracic surgery	855:880	PATIENTS Adults who had non-cardiothoracic surgery and were given either neostigmine or sugammadex.
37148836	8	42	dep	None	945:948	arg1	INTERVENTIONS					931:943	INTERVENTIONS	931:943	INTERVENTIONS	931:943	INTERVENTIONS None.
37148836	2	43	theme	neuromuscular	394:406	arg1	function					408:415	neuromuscular function	394:415	neuromuscular function	394:415	Sugammadex may provide more rapid and effective restoration of neuromuscular function than neostigmine.
37148836	1	44	theme	inadequate	272:281	arg1	oxygenation					283:293	inadequate oxygenation	272:293	inadequate oxygenation	272:293	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	0	45	with	reversal	90:97	arg1	neostigmine					141:151	neostigmine	141:151	neostigmine	141:151	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	0	45	with	reversal	90:97	arg1	Sugammadex					127:136	Sugammadex	127:136	Sugammadex	127:136	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	14	46	theme	SpO2/FiO2	1797:1805	arg1	ratio					1807:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio	1763:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission	1763:1833	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission was similar after reversal of neuromuscular block by sugammadex and neostigmine.
37148836	12	47	from	difference	1393:1402	arg1	means					1407:1411	means	1407:1411	means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002)	1407:1469	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	9	48	from	ratio	1009:1013	arg1	unit					1043:1046	the post-anesthesia care unit	1018:1046	the post-anesthesia care unit	1018:1046	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	1	49	theme	STUDY	184:188	arg1	block					223:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	5	50	theme	Retrospective	735:747	arg1	analysis					756:763	Retrospective cohort analysis	735:763	DESIGN Retrospective cohort analysis.	728:764	DESIGN Retrospective cohort analysis.
37148836	9	51	theme	care	1038:1041	arg1	unit					1043:1046	the post-anesthesia care unit	1018:1046	the post-anesthesia care unit	1018:1046	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	1	52	theme	Residual	200:207	arg1	block					223:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block	184:227	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	5	53	theme	cohort	749:754	arg1	analysis					756:763	Retrospective cohort analysis	735:763	DESIGN Retrospective cohort analysis.	728:764	DESIGN Retrospective cohort analysis.
37148836	9	54	dep	MEASUREMENTS	951:962	arg1	outcome					976:982	The primary outcome	964:982	MEASUREMENTS The primary outcome	951:982	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	14	55	theme	CONCLUSIONS	1763:1773	arg1	ratio					1807:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio	1763:1811	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission	1763:1833	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission was similar after reversal of neuromuscular block by sugammadex and neostigmine.
37148836	4	56	contain	have	669:672	arg2	complications					690:702	fewer pulmonary complications	674:702	fewer pulmonary complications	674:702	Secondarily, we tested the hypothesis that patients given sugammadex have fewer pulmonary complications during hospitalization.
37148836	4	56	contain	have	669:672	arg1	patients					643:650	patients	643:650	patients given sugammadex	643:667	Secondarily, we tested the hypothesis that patients given sugammadex have fewer pulmonary complications during hospitalization.
37148836	0	57	theme	cohort	170:175	arg1	study					177:181	A retrospective cohort study	154:181	A retrospective cohort study	154:181	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	12	58	theme	interval	1437:1444	arg1	-3.5					1416:1419	-3.5	1416:1419	-3.5	1416:1419	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	12	58	theme	interval	1437:1444	arg1	%					1424:1424	95%	1422:1424	95% confidence interval	1422:1444	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	6	59	theme	Postoperative	774:786	arg1	area					797:800	Postoperative recovery area	774:800	Postoperative recovery area of a tertiary care hospital	774:828	SETTING Postoperative recovery area of a tertiary care hospital.
37148836	12	60	theme	SpO2/FiO2	1267:1275	arg1	301 ± 77					1287:1294	301 ± 77	1287:1294	301 ± 77	1287:1294	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	12	60	theme	SpO2/FiO2	1267:1275	arg1	ratio					1277:1281	the mean minimum SpO2/FiO2 ratio	1250:1281	the mean minimum SpO2/FiO2 ratio	1250:1281	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	0	61	theme	block	116:120	arg1	reversal					90:97	reversal	90:97	reversal of neuromuscular block with Sugammadex or neostigmine	90:151	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	12	62	from	301 ± 77	1287:1294	arg1	patients					1304:1311	patients	1304:1311	patients given sugammadex and 303 ± 71 in those given neostigmine	1304:1368	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	9	63	theme	primary	968:974	arg1	outcome					976:982	The primary outcome	964:982	MEASUREMENTS The primary outcome	951:982	MEASUREMENTS The primary outcome was the lowest SpO2/FiO2 ratio in the post-anesthesia care unit.
37148836	2	64	theme	rapid	359:363	arg1	restoration					379:389	more rapid and effective restoration	354:389	more rapid and effective restoration of neuromuscular function than neostigmine	354:432	Sugammadex may provide more rapid and effective restoration of neuromuscular function than neostigmine.
37148836	10	65	theme	secondary	1053:1061	arg1	outcome					1063:1069	The secondary outcome	1049:1069	The secondary outcome	1049:1069	The secondary outcome was a composite of pulmonary complications.
37148836	10	65	theme	secondary	1053:1061	arg1	composite					1077:1085	a composite	1075:1085	a composite of pulmonary complications	1075:1112	The secondary outcome was a composite of pulmonary complications.
37148836	10	65	theme	secondary	1053:1061	arg1	complications					1100:1112	pulmonary complications	1090:1112	pulmonary complications	1090:1112	The secondary outcome was a composite of pulmonary complications.
37148836	6	66	theme	care	816:819	arg1	hospital					821:828	a tertiary care hospital	805:828	a tertiary care hospital	805:828	SETTING Postoperative recovery area of a tertiary care hospital.
37148836	13	67	theme	postoperative	1549:1561	arg1	P = 0.0005					1588:1597	P = 0.0005	1588:1597	P = 0.0005	1588:1597	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	67	theme	postoperative	1549:1561	arg1	complications					1573:1585	postoperative pulmonary complications	1549:1585	postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330)	1549:1649	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	10	68	theme	pulmonary	1090:1098	arg1	complications					1100:1112	pulmonary complications	1090:1112	pulmonary complications	1090:1112	The secondary outcome was a composite of pulmonary complications.
37148836	13	69	theme	pulmonary	1744:1752	arg1	disease					1754:1760	obstructive pulmonary disease	1732:1760	obstructive pulmonary disease	1732:1760	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	3	70	theme	noncardiac	483:492	arg1	patients					503:510	noncardiac surgical patients	483:510	noncardiac surgical patients given sugammadex	483:527	We therefore tested the primary hypothesis that noncardiac surgical patients given sugammadex oxygenate better during initial recovery than those given neostigmine.
37148836	12	71	theme	minimum	1259:1265	arg1	301 ± 77					1287:1294	301 ± 77	1287:1294	301 ± 77	1287:1294	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	12	71	theme	minimum	1259:1265	arg1	ratio					1277:1281	the mean minimum SpO2/FiO2 ratio	1250:1281	the mean minimum SpO2/FiO2 ratio	1250:1281	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	1	72	theme	pulmonary	306:314	arg1	complications					316:328	other pulmonary complications	300:328	other pulmonary complications	300:328	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	13	73	theme	new	1696:1698	arg1	components					1679:1688	the main contributing components	1657:1688	the main contributing components	1657:1688	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	73	theme	new	1696:1698	arg1	bronchospasm					1700:1711	new bronchospasm	1696:1711	new bronchospasm	1696:1711	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	74	theme	patients	1518:1525	arg1	patients					1480:1487	patients	1480:1487	patients given sugammadex	1480:1504	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	74	theme	patients	1518:1525	arg1	%					1475:1475	4.4%	1472:1475	4.4% of patients given sugammadex	1472:1504	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	74	theme	patients	1518:1525	arg1	patients					1518:1525	patients	1518:1525	patients given neostigmine	1518:1543	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	74	theme	patients	1518:1525	arg1	%					1513:1513	3.6%	1510:1513	3.6% of patients given neostigmine	1510:1543	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	14	75	theme	block	1879:1883	arg1	reversal					1853:1860	reversal	1853:1860	reversal of neuromuscular block by sugammadex and neostigmine	1853:1913	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission was similar after reversal of neuromuscular block by sugammadex and neostigmine.
37148836	12	76	dep	%	1424:1424	arg1	-5.3					1447:1450	-5.3	1447:1450	-5.3	1447:1450	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	12	76	dep	%	1424:1424	arg1	P = 0.0002					1459:1468	P = 0.0002	1459:1468	P = 0.0002	1459:1468	After propensity weighting, the mean minimum SpO2/FiO2 ratio was 301 ± 77 (SD) in patients given sugammadex and 303 ± 71 in those given neostigmine, yielding an estimated difference in means of -3.5 (95% confidence interval: -5.3, -1.7; P = 0.0002).
37148836	0	77	theme	respiratory	58:68	arg1	complications					70:82	respiratory complications	58:82	respiratory complications	58:82	Postoperative oxygenation assessed by SpO2/FiO2 ratio and respiratory complications after reversal of neuromuscular block with Sugammadex or neostigmine: A retrospective cohort study.
37148836	15	78	with	Reversal	1916:1923	arg1	sugammadex					1930:1939	sugammadex	1930:1939	sugammadex	1930:1939	Reversal with sugammadex was associated with more pulmonary complications, but most were minor and of little consequence.
37148836	5	79	dep	DESIGN	728:733	arg1	analysis					756:763	Retrospective cohort analysis	735:763	DESIGN Retrospective cohort analysis.	728:764	DESIGN Retrospective cohort analysis.
37148836	13	80	theme	main	1661:1664	arg1	components					1679:1688	the main contributing components	1657:1688	the main contributing components	1657:1688	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	13	80	theme	main	1661:1664	arg1	bronchospasm					1700:1711	new bronchospasm	1696:1711	new bronchospasm	1696:1711	4.4% of patients given sugammadex and 3.6% of patients given neostigmine had postoperative pulmonary complications (P = 0.0005, number-needed-to-be-exposed =136; 95% CI: 83, 330), with the main contributing components being new bronchospasm or exacerbation of obstructive pulmonary disease.
37148836	2	81	theme	effective	369:377	arg1	restoration					379:389	more rapid and effective restoration	354:389	more rapid and effective restoration of neuromuscular function than neostigmine	354:432	Sugammadex may provide more rapid and effective restoration of neuromuscular function than neostigmine.
37148836	3	82	theme	initial	553:559	arg1	recovery					561:568	initial recovery	553:568	initial recovery	553:568	We therefore tested the primary hypothesis that noncardiac surgical patients given sugammadex oxygenate better during initial recovery than those given neostigmine.
37148836	1	83	theme	muscle	255:260	arg1	weakness					262:269	postoperative muscle weakness	241:269	postoperative muscle weakness	241:269	STUDY OBJECTIVE Residual neuromuscular block may lead to postoperative muscle weakness, inadequate oxygenation, and other pulmonary complications.
37148836	2	84	theme	function	408:415	arg1	restoration					379:389	more rapid and effective restoration	354:389	more rapid and effective restoration of neuromuscular function than neostigmine	354:432	Sugammadex may provide more rapid and effective restoration of neuromuscular function than neostigmine.
37148836	14	85	theme	PACU	1820:1823	arg1	admission					1825:1833	PACU admission	1820:1833	PACU admission	1820:1833	CONCLUSIONS Postoperative minimum SpO2/FiO2 ratio during PACU admission was similar after reversal of neuromuscular block by sugammadex and neostigmine.
35227303	4	0	theme	poplar	937:942	arg1	poplar					937:942	milled non-pretreated poplar	915:942	milled non-pretreated poplar	915:942	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	4	0	theme	poplar	937:942	arg1	%					910:910	5.8, 10.3, and 13.7%	891:910	5.8, 10.3, and 13.7% of milled non-pretreated poplar	891:942	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	6	1	theme	non-cellulosic	1141:1154	arg1	sugars					1156:1161	non-cellulosic sugars	1141:1161	non-cellulosic sugars	1141:1161	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	10	2	theme	solid	1976:1980	arg1	residues					1982:1989	the solid residues	1972:1989	the solid residues	1972:1989	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	13	3	theme	pectic	2619:2624	arg1	glycan					2626:2631	this pectic glycan	2614:2631	this pectic glycan	2614:2631	These results support the hypothesis that this pectic glycan may be one barrier for efficient solubilization of poplar by C. thermocellum.
35227303	11	4	dep	liquor	2200:2205	arg1	the					2196:2198	the	2196:2198	the	2196:2198	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	3	5	theme	cellulosic	626:635	arg1	polysaccharides					665:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	8	6	theme	wall	1615:1618	arg1	polymers					1620:1627	the non-cellulosic wall polymers	1596:1627	the non-cellulosic wall polymers	1596:1627	These results indicate that C. thermocellum may be selective for the types and/or rate of solubilization of the non-cellulosic wall polymers.
35227303	6	7	theme	xylose	1278:1283	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	Man					1318:1320	Man	1318:1320	Man	1318:1320	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	acid					1383:1386	glucuronic acid	1372:1386	glucuronic acid	1372:1386	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	Glc					1337:1339	Glc	1337:1339	Glc	1337:1339	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	Gal					1303:1305	Gal	1303:1305	Gal	1303:1305	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	%					1356:1356	3%	1355:1356	3%	1355:1356	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	Xyl					1286:1288	Xyl	1286:1288	Xyl	1286:1288	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	Ara					1272:1274	Ara	1272:1274	Ara	1272:1274	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	%					1349:1349	15%	1347:1349	15%	1347:1349	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	7	theme	xylose	1278:1283	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	3	8	theme	cell	616:619	arg1	polysaccharides					665:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	1	9	theme	plant	203:207	arg1	biomass					209:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	1	9	theme	plant	203:207	arg1	feedstock					249:257	the primary renewable carbon feedstock	220:257	the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy	220:309	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	4	10	theme	milled	915:920	arg1	poplar					937:942	milled non-pretreated poplar	915:942	milled non-pretreated poplar	915:942	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	6	11	theme	uninoculated	1208:1219	arg1	fermentations					1229:1241	the uninoculated control fermentations	1204:1241	the uninoculated control fermentations	1204:1241	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	11	12	from	sugars	2186:2191	arg1	liquor					2200:2205	liquor	2200:2205	liquor	2200:2205	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	11	12	from	sugars	2186:2191	arg1	residue					2220:2226	solid residue	2214:2226	solid residue	2214:2226	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	10	13	theme	Rha-containing	2025:2038	arg1	RG-I					2070:2073	RG-I	2070:2073	RG-I	2070:2073	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	10	13	theme	Rha-containing	2025:2038	arg1	I					2067:2067	the Rha-containing polymer rhamnogalacturonan I	2021:2067	the Rha-containing polymer rhamnogalacturonan I (RG-I)	2021:2074	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	3	14	theme	hemicellulosic	638:651	arg1	polysaccharides					665:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	1	15	theme	primary	224:230	arg1	biomass					209:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	1	15	theme	primary	224:230	arg1	feedstock					249:257	the primary renewable carbon feedstock	220:257	the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy	220:309	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	0	16	theme	biomass	143:149	arg1	bioprocessing					119:131	consolidated bioprocessing	106:131	consolidated bioprocessing of poplar biomass by Clostridium thermocellum	106:177	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	1	17	theme	carbon	242:247	arg1	biomass					209:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	1	17	theme	carbon	242:247	arg1	feedstock					249:257	the primary renewable carbon feedstock	220:257	the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy	220:309	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	6	18	theme	arabinose	1261:1269	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	Man					1318:1320	Man	1318:1320	Man	1318:1320	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	acid					1383:1386	glucuronic acid	1372:1386	glucuronic acid	1372:1386	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	Glc					1337:1339	Glc	1337:1339	Glc	1337:1339	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	Gal					1303:1305	Gal	1303:1305	Gal	1303:1305	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	%					1356:1356	3%	1355:1356	3%	1355:1356	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	Xyl					1286:1288	Xyl	1286:1288	Xyl	1286:1288	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	Ara					1272:1274	Ara	1272:1274	Ara	1272:1274	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	%					1349:1349	15%	1347:1349	15%	1347:1349	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	18	theme	arabinose	1261:1269	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	12	19	theme	C.	2531:2532	arg1	thermocellum					2534:2545	C. thermocellum	2531:2545	C. thermocellum	2531:2545	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	0	20	theme	Clostridium	154:164	arg1	thermocellum					166:177	Clostridium thermocellum	154:177	Clostridium thermocellum	154:177	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	3	21	theme	polysaccharides	665:679	arg1	solubilization					598:611	solubilization	598:611	solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum	598:770	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	10	22	theme	rhamnogalacturonan	2048:2065	arg1	RG-I					2070:2073	RG-I	2070:2073	RG-I	2070:2073	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	10	22	theme	rhamnogalacturonan	2048:2065	arg1	I					2067:2067	the Rha-containing polymer rhamnogalacturonan I	2021:2067	the Rha-containing polymer rhamnogalacturonan I (RG-I)	2021:2074	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	5	23	theme	prior	1100:1104	arg1	reports					1106:1112	prior reports	1100:1112	prior reports	1100:1112	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	0	24	from	yield	16:20	arg1	fractions					89:97	soluble and particulate fractions	65:97	soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum	65:177	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	13	25	theme	poplar	2684:2689	arg1	solubilization					2666:2679	efficient solubilization	2656:2679	efficient solubilization of poplar by C. thermocellum	2656:2708	These results support the hypothesis that this pectic glycan may be one barrier for efficient solubilization of poplar by C. thermocellum.
35227303	1	26	theme	BACKGROUND	180:189	arg1	biomass					209:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	1	26	theme	BACKGROUND	180:189	arg1	feedstock					249:257	the primary renewable carbon feedstock	220:257	the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy	220:309	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	0	27	from	Composition	0:10	arg1	fractions					89:97	soluble and particulate fractions	65:97	soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum	65:177	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	10	28	theme	C.	2111:2112	arg1	CBP					2127:2129	C. thermocellum CBP	2111:2129	C. thermocellum CBP	2111:2129	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	10	28	theme	C.	2111:2112	arg1	result					2134:2139	a result	2132:2139	a result confirmed by immunoassays	2132:2165	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	4	29	theme	C.	841:842	arg1	platform					878:885	the C. thermocellum DSM1313 fermentation platform	837:885	the C. thermocellum DSM1313 fermentation platform	837:885	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	10	30	theme	fermentation	1928:1939	arg1	days					1920:1923	five days	1915:1923	five days of fermentation	1915:1939	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	6	31	theme	glucose	1328:1334	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	Man					1318:1320	Man	1318:1320	Man	1318:1320	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	acid					1383:1386	glucuronic acid	1372:1386	glucuronic acid	1372:1386	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	Glc					1337:1339	Glc	1337:1339	Glc	1337:1339	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	Gal					1303:1305	Gal	1303:1305	Gal	1303:1305	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	%					1356:1356	3%	1355:1356	3%	1355:1356	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	Xyl					1286:1288	Xyl	1286:1288	Xyl	1286:1288	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	Ara					1272:1274	Ara	1272:1274	Ara	1272:1274	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	%					1349:1349	15%	1347:1349	15%	1347:1349	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	31	theme	glucose	1328:1334	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	11	32	from	residue	2220:2226	arg1	Comparison					2168:2177	Comparison	2168:2177	Comparison of the sugars in the liquor versus solid residue	2168:2226	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	5	33	theme	24	1027:1028	arg1	%					1029:1029	%	1029:1029	%	1029:1029	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	4	34	theme	total	798:802	arg1	biomass					814:820	biomass	814:820	biomass	814:820	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	4	34	theme	total	798:802	arg1	amount					804:809	The total amount	794:809	The total amount of biomass	794:820	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	12	35	theme	solid	2487:2491	arg1	residues					2493:2500	poplar solid residues	2480:2500	poplar solid residues following CBP	2480:2514	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	4	36	theme	DSM1313	857:863	arg1	platform					878:885	the C. thermocellum DSM1313 fermentation platform	837:885	the C. thermocellum DSM1313 fermentation platform	837:885	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	0	37	theme	consolidated	106:117	arg1	bioprocessing					119:131	consolidated bioprocessing	106:131	consolidated bioprocessing of poplar biomass by Clostridium thermocellum	106:177	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	5	38	theme	cellulose	1031:1039	arg1	sugars					1064:1069	24% cellulose and 17% non-cellulosic sugars	1027:1069	24% cellulose and 17% non-cellulosic sugars	1027:1069	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	6	39	theme	net	1119:1121	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	39	theme	net	1119:1121	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	39	theme	net	1119:1121	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	39	theme	net	1119:1121	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	39	theme	net	1119:1121	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	39	theme	net	1119:1121	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	39	theme	net	1119:1121	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	39	theme	net	1119:1121	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	9	40	theme	fermentation	1680:1691	arg1	liquor					1693:1698	the fermentation liquor	1676:1698	the fermentation liquor	1676:1698	Xyl, Man, and Glc were found to accumulate in the fermentation liquor at levels greater than in uninoculated control fermentations, whereas Ara and Gal did not accumulate, suggesting that C. thermocellum solubilizes both hemicelluloses and pectins but utilizes them differently.
35227303	12	41	theme	relative	2451:2458	arg1	increase					2460:2467	The significant relative increase	2435:2467	The significant relative increase in RG-I in poplar solid residues following CBP	2435:2514	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	4	42	theme	biomass	814:820	arg1	biomass					814:820	biomass	814:820	biomass	814:820	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	4	42	theme	biomass	814:820	arg1	amount					804:809	The total amount	794:809	The total amount of biomass	794:820	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	Man					1318:1320	Man	1318:1320	Man	1318:1320	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	acid					1383:1386	glucuronic acid	1372:1386	glucuronic acid	1372:1386	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	Glc					1337:1339	Glc	1337:1339	Glc	1337:1339	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	Gal					1303:1305	Gal	1303:1305	Gal	1303:1305	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	%					1356:1356	3%	1355:1356	3%	1355:1356	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	Xyl					1286:1288	Xyl	1286:1288	Xyl	1286:1288	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	Ara					1272:1274	Ara	1272:1274	Ara	1272:1274	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	%					1349:1349	15%	1347:1349	15%	1347:1349	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	43	theme	fucose	1361:1366	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	12	44	dep	CONCLUSIONS	2423:2433	arg1	indicates					2516:2524	indicates	2516:2524	indicates that C. thermocellum did not solubilize RG-I	2516:2569	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	2	45	theme	simultaneous	490:501	arg1	solubilization					503:516	simultaneous solubilization	490:516	simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	490:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	2	46	theme	biochemicals	473:484	arg1	production					446:455	production	446:455	production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	446:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	7	47	theme	galacturonic	1446:1457	arg1	GalA					1465:1468	GalA	1465:1468	GalA	1465:1468	No rhamnose was solubilized and 71% of the galacturonic acid (GalA) was solubilized.
35227303	7	47	theme	galacturonic	1446:1457	arg1	acid					1459:1462	the galacturonic acid	1442:1462	the galacturonic acid (GalA)	1442:1469	No rhamnose was solubilized and 71% of the galacturonic acid (GalA) was solubilized.
35227303	4	48	dep	RESULTS	786:792	arg1	solubilized					822:832	solubilized	822:832	solubilized in the C. thermocellum DSM1313 fermentation platform	822:885	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	9	49	theme	greater	1710:1716	arg1	levels					1703:1708	levels	1703:1708	levels greater than in uninoculated control fermentations	1703:1759	Xyl, Man, and Glc were found to accumulate in the fermentation liquor at levels greater than in uninoculated control fermentations, whereas Ara and Gal did not accumulate, suggesting that C. thermocellum solubilizes both hemicelluloses and pectins but utilizes them differently.
35227303	2	50	theme	biofuels	460:467	arg1	production					446:455	production	446:455	production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	446:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	0	51	theme	non-cellulosic	25:38	arg1	sugars					55:60	non-cellulosic and cellulosic sugars	25:60	non-cellulosic and cellulosic sugars	25:60	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	0	52	theme	cellulosic	44:53	arg1	sugars					55:60	non-cellulosic and cellulosic sugars	25:60	non-cellulosic and cellulosic sugars	25:60	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	9	53	theme	control	1739:1745	arg1	fermentations					1747:1759	uninoculated control fermentations	1726:1759	uninoculated control fermentations	1726:1759	Xyl, Man, and Glc were found to accumulate in the fermentation liquor at levels greater than in uninoculated control fermentations, whereas Ara and Gal did not accumulate, suggesting that C. thermocellum solubilizes both hemicelluloses and pectins but utilizes them differently.
35227303	2	54	theme	thermophile	365:375	arg1	thermocellum					389:400	the cellulolytic thermophile Clostridium thermocellum	348:400	the cellulolytic thermophile Clostridium thermocellum	348:400	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	12	55	theme	significant	2439:2449	arg1	increase					2460:2467	The significant relative increase	2435:2467	The significant relative increase in RG-I in poplar solid residues following CBP	2435:2514	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	3	56	theme	biomass	745:751	arg1	biomass					745:751	poplar biomass	738:751	poplar biomass	738:751	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	3	56	theme	biomass	745:751	arg1	liquor					688:693	liquor	688:693	liquor	688:693	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	3	56	theme	biomass	745:751	arg1	residues					705:712	solid residues	699:712	solid residues generated during CBP	699:733	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	2	57	theme	step	418:421	arg1	platform					433:440	a single step microbial platform	409:440	a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	409:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	5	58	theme	non-cellulosic	1049:1062	arg1	sugars					1064:1069	24% cellulose and 17% non-cellulosic sugars	1027:1069	24% cellulose and 17% non-cellulosic sugars	1027:1069	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	6	59	theme	sugars	1156:1161	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	59	theme	sugars	1156:1161	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	59	theme	sugars	1156:1161	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	59	theme	sugars	1156:1161	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	59	theme	sugars	1156:1161	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	59	theme	sugars	1156:1161	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	59	theme	sugars	1156:1161	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	59	theme	sugars	1156:1161	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	Man					1318:1320	Man	1318:1320	Man	1318:1320	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	acid					1383:1386	glucuronic acid	1372:1386	glucuronic acid	1372:1386	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	Glc					1337:1339	Glc	1337:1339	Glc	1337:1339	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	Gal					1303:1305	Gal	1303:1305	Gal	1303:1305	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	%					1356:1356	3%	1355:1356	3%	1355:1356	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	Xyl					1286:1288	Xyl	1286:1288	Xyl	1286:1288	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	Ara					1272:1274	Ara	1272:1274	Ara	1272:1274	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	%					1349:1349	15%	1347:1349	15%	1347:1349	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	60	theme	galactose	1292:1300	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	12	61	from	increase	2460:2467	arg1	RG-I					2472:2475	RG-I	2472:2475	RG-I	2472:2475	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	12	61	from	increase	2460:2467	arg1	residues					2493:2500	poplar solid residues	2480:2500	poplar solid residues following CBP	2480:2514	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	4	62	theme	non-pretreated	922:935	arg1	poplar					937:942	milled non-pretreated poplar	915:942	milled non-pretreated poplar	915:942	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	11	63	theme	solid	2214:2218	arg1	residue					2220:2226	solid residue	2214:2226	solid residue	2214:2226	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	3	64	theme	wall	621:624	arg1	polysaccharides					665:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	6	65	theme	glucuronic	1372:1381	arg1	acid					1383:1386	glucuronic acid	1372:1386	glucuronic acid	1372:1386	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	1	66	theme	sustainable	288:298	arg1	bioeconomy					300:309	a sustainable bioeconomy	286:309	a sustainable bioeconomy	286:309	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	3	67	from	solubilization	598:611	arg1	biomass					745:751	poplar biomass	738:751	poplar biomass	738:751	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	3	67	from	solubilization	598:611	arg1	liquor					688:693	liquor	688:693	liquor	688:693	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	3	67	from	solubilization	598:611	arg1	residues					705:712	solid residues	699:712	solid residues generated during CBP	699:733	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	2	68	theme	Consolidated	312:323	arg1	bioprocessing					325:337	Consolidated bioprocessing	312:337	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum	312:400	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	2	68	theme	Consolidated	312:323	arg1	CBP					340:342	CBP	340:342	CBP	340:342	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	8	69	theme	non-cellulosic	1600:1613	arg1	polymers					1620:1627	the non-cellulosic wall polymers	1596:1627	the non-cellulosic wall polymers	1596:1627	These results indicate that C. thermocellum may be selective for the types and/or rate of solubilization of the non-cellulosic wall polymers.
35227303	1	70	theme	Terrestrial	191:201	arg1	biomass					209:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	1	70	theme	Terrestrial	191:201	arg1	feedstock					249:257	the primary renewable carbon feedstock	220:257	the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy	220:309	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	9	71	theme	uninoculated	1726:1737	arg1	fermentations					1747:1759	uninoculated control fermentations	1726:1759	uninoculated control fermentations	1726:1759	Xyl, Man, and Glc were found to accumulate in the fermentation liquor at levels greater than in uninoculated control fermentations, whereas Ara and Gal did not accumulate, suggesting that C. thermocellum solubilizes both hemicelluloses and pectins but utilizes them differently.
35227303	6	72	theme	control	1221:1227	arg1	fermentations					1229:1241	the uninoculated control fermentations	1204:1241	the uninoculated control fermentations	1204:1241	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	8	73	theme	polymers	1620:1627	arg1	solubilization					1578:1591	solubilization	1578:1591	solubilization of the non-cellulosic wall polymers	1578:1627	These results indicate that C. thermocellum may be selective for the types and/or rate of solubilization of the non-cellulosic wall polymers.
35227303	10	74	theme	polymer	2040:2046	arg1	RG-I					2070:2073	RG-I	2070:2073	RG-I	2070:2073	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	10	74	theme	polymer	2040:2046	arg1	I					2067:2067	the Rha-containing polymer rhamnogalacturonan I	2021:2067	the Rha-containing polymer rhamnogalacturonan I (RG-I)	2021:2074	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	0	75	theme	poplar	136:141	arg1	biomass					143:149	poplar biomass	136:149	poplar biomass	136:149	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	11	76	from	liquor	2200:2205	arg1	Comparison					2168:2177	Comparison	2168:2177	Comparison of the sugars in the liquor versus solid residue	2168:2226	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	3	77	dep	liquor	688:693	arg1	the					684:686	the	684:686	the	684:686	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	2	78	from	conversion	568:577	arg1	solubilization					503:516	simultaneous solubilization	490:516	simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	490:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	11	79	from	Comparison	2168:2177	arg1	liquor					2200:2205	liquor	2200:2205	liquor	2200:2205	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	11	79	from	Comparison	2168:2177	arg1	residue					2220:2226	solid residue	2214:2226	solid residue	2214:2226	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	1	80	theme	renewable	232:240	arg1	biomass					209:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass	180:215	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	1	80	theme	renewable	232:240	arg1	feedstock					249:257	the primary renewable carbon feedstock	220:257	the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy	220:309	BACKGROUND Terrestrial plant biomass is the primary renewable carbon feedstock for enabling transition to a sustainable bioeconomy.
35227303	10	81	theme	Rha	1965:1967	arg1	Rha					1965:1967	Rha	1965:1967	Rha	1965:1967	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	10	81	theme	Rha	1965:1967	arg1	amount					1955:1960	the relative amount	1942:1960	the relative amount of Rha in the solid residues	1942:1989	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	3	82	theme	pectic	658:663	arg1	polysaccharides					665:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	cell wall cellulosic, hemicellulosic, and pectic polysaccharides	616:679	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	3	83	theme	solid	699:703	arg1	biomass					745:751	poplar biomass	738:751	poplar biomass	738:751	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	3	83	theme	solid	699:703	arg1	residues					705:712	solid residues	699:712	solid residues generated during CBP	699:733	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	11	84	theme	hemicellulosic	2268:2281	arg1	xylan					2283:2287	hemicellulosic xylan	2268:2287	hemicellulosic xylan	2268:2287	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	10	85	from	amount	1955:1960	arg1	residues					1982:1989	the solid residues	1972:1989	the solid residues	1972:1989	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	6	86	dep	36	1254:1255	arg1	to					1251:1252	to	1251:1252	to	1251:1252	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	0	87	theme	soluble	65:71	arg1	fractions					89:97	soluble and particulate fractions	65:97	soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum	65:177	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	8	88	dep	types	1557:1561	arg1	the					1553:1555	the	1553:1555	the	1553:1555	These results indicate that C. thermocellum may be selective for the types and/or rate of solubilization of the non-cellulosic wall polymers.
35227303	0	89	theme	particulate	77:87	arg1	fractions					89:97	soluble and particulate fractions	65:97	soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum	65:177	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	4	90	dep	C.	841:842	arg1	thermocellum					844:855	thermocellum	844:855	thermocellum	844:855	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	12	91	theme	poplar	2480:2485	arg1	residues					2493:2500	poplar solid residues	2480:2500	poplar solid residues following CBP	2480:2514	CONCLUSIONS The significant relative increase in RG-I in poplar solid residues following CBP indicates that C. thermocellum did not solubilize RG-I.
35227303	10	92	dep	C.	2111:2112	arg1	thermocellum					2114:2125	thermocellum	2114:2125	thermocellum	2114:2125	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	5	93	theme	%	1029:1029	arg1	cellulose					1031:1039	24% cellulose	1027:1039	24% cellulose	1027:1039	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	2	94	from	biomass	556:562	arg1	solubilization					503:516	simultaneous solubilization	490:516	simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	490:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	5	95	with	consistent	1084:1093	arg1	reports					1106:1112	prior reports	1100:1112	prior reports	1100:1112	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	13	96	theme	efficient	2656:2664	arg1	solubilization					2666:2679	efficient solubilization	2656:2679	efficient solubilization of poplar by C. thermocellum	2656:2708	These results support the hypothesis that this pectic glycan may be one barrier for efficient solubilization of poplar by C. thermocellum.
35227303	11	97	dep	utilize	2319:2325	arg1	appeared					2349:2356	appeared	2349:2356	appeared to utilize pectic homogalacturonan	2349:2391	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	11	97	dep	utilize	2319:2325	arg1	solubilized					2333:2343	solubilized	2333:2343	solubilized	2333:2343	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	2	98	theme	microbial	423:431	arg1	platform					433:440	a single step microbial platform	409:440	a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	409:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	11	99	theme	sugars	2186:2191	arg1	Comparison					2168:2177	Comparison	2168:2177	Comparison of the sugars in the liquor versus solid residue	2168:2226	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	7	100	theme	acid	1459:1462	arg1	%					1437:1437	71%	1435:1437	71% of the galacturonic acid (GalA)	1435:1469	No rhamnose was solubilized and 71% of the galacturonic acid (GalA) was solubilized.
35227303	7	100	theme	acid	1459:1462	arg1	GalA					1465:1468	GalA	1465:1468	GalA	1465:1468	No rhamnose was solubilized and 71% of the galacturonic acid (GalA) was solubilized.
35227303	7	100	theme	acid	1459:1462	arg1	acid					1459:1462	the galacturonic acid	1442:1462	the galacturonic acid (GalA)	1442:1469	No rhamnose was solubilized and 71% of the galacturonic acid (GalA) was solubilized.
35227303	5	101	theme	sugars	1064:1069	arg1	solubilization					1009:1022	solubilization	1009:1022	solubilization of 24% cellulose and 17% non-cellulosic sugars	1009:1069	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	2	102	theme	lignocellulosic	540:554	arg1	biomass					556:562	lignocellulosic biomass	540:562	lignocellulosic biomass	540:562	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	0	103	theme	sugars	55:60	arg1	yield					16:20	yield	16:20	yield	16:20	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	0	103	theme	sugars	55:60	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition and yield of non-cellulosic and cellulosic sugars in soluble and particulate fractions during consolidated bioprocessing of poplar biomass by Clostridium thermocellum.
35227303	11	104	theme	pectic	2369:2374	arg1	homogalacturonan					2376:2391	pectic homogalacturonan	2369:2391	pectic homogalacturonan	2369:2391	Comparison of the sugars in the liquor versus solid residue showed that C. thermocellum solubilized hemicellulosic xylan and mannan, but did not fully utilize them, solubilized and appeared to utilize pectic homogalacturonan, and did not solubilize RG-I.
35227303	2	105	theme	carbohydrates	521:533	arg1	solubilization					503:516	simultaneous solubilization	490:516	simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	490:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	2	106	theme	Clostridium	377:387	arg1	thermocellum					389:400	the cellulolytic thermophile Clostridium thermocellum	348:400	the cellulolytic thermophile Clostridium thermocellum	348:400	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	6	107	theme	mannose	1309:1315	arg1	%					1256:1256	13 to 36%	1248:1256	13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose	1248:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	Man					1318:1320	Man	1318:1320	Man	1318:1320	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	acid					1383:1386	glucuronic acid	1372:1386	glucuronic acid	1372:1386	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	Glc					1337:1339	Glc	1337:1339	Glc	1337:1339	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	xylose					1278:1283	xylose	1278:1283	xylose (Xyl)	1278:1289	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	Gal					1303:1305	Gal	1303:1305	Gal	1303:1305	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	%					1356:1356	3%	1355:1356	3%	1355:1356	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	arabinose					1261:1269	arabinose	1261:1269	arabinose (Ara)	1261:1275	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	fucose					1361:1366	fucose	1361:1366	fucose	1361:1366	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	Xyl					1286:1288	Xyl	1286:1288	Xyl	1286:1288	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	Ara					1272:1274	Ara	1272:1274	Ara	1272:1274	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	glucose					1328:1334	glucose	1328:1334	glucose	1328:1334	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	solubilization					1123:1136	The net solubilization	1115:1136	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations)	1115:1242	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	%					1349:1349	15%	1347:1349	15%	1347:1349	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	mannose					1309:1315	mannose	1309:1315	mannose (Man)	1309:1321	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	6	107	theme	mannose	1309:1315	arg1	galactose					1292:1300	galactose	1292:1300	galactose (Gal)	1292:1306	The net solubilization of non-cellulosic sugars by C. thermocellum (after correcting for the uninoculated control fermentations) was 13 to 36% of arabinose (Ara), xylose (Xyl), galactose (Gal), mannose (Man), and glucose (Glc); and 15% and 3% of fucose and glucuronic acid, respectively.
35227303	3	108	theme	poplar	738:743	arg1	biomass					745:751	poplar biomass	738:751	poplar biomass	738:751	Here, solubilization of cell wall cellulosic, hemicellulosic, and pectic polysaccharides in the liquor and solid residues generated during CBP of poplar biomass by C. thermocellum was analyzed.
35227303	4	109	theme	fermentation	865:876	arg1	platform					878:885	the C. thermocellum DSM1313 fermentation platform	837:885	the C. thermocellum DSM1313 fermentation platform	837:885	RESULTS The total amount of biomass solubilized in the C. thermocellum DSM1313 fermentation platform was 5.8, 10.3, and 13.7% of milled non-pretreated poplar after 24, 48, and 120 h, respectively.
35227303	2	110	theme	cellulolytic	352:363	arg1	thermocellum					389:400	the cellulolytic thermophile Clostridium thermocellum	348:400	the cellulolytic thermophile Clostridium thermocellum	348:400	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	5	111	theme	%	1047:1047	arg1	sugars					1064:1069	24% cellulose and 17% non-cellulosic sugars	1027:1069	24% cellulose and 17% non-cellulosic sugars	1027:1069	These results demonstrate solubilization of 24% cellulose and 17% non-cellulosic sugars after 120 h, consistent with prior reports.
35227303	10	112	theme	relative	1946:1953	arg1	Rha					1965:1967	Rha	1965:1967	Rha	1965:1967	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	10	112	theme	relative	1946:1953	arg1	amount					1955:1960	the relative amount	1942:1960	the relative amount of Rha in the solid residues	1942:1989	After five days of fermentation, the relative amount of Rha in the solid residues increased 21% indicating that the Rha-containing polymer rhamnogalacturonan I (RG-I) was not effectively solubilized by C. thermocellum CBP, a result confirmed by immunoassays.
35227303	2	113	theme	single	411:416	arg1	platform					433:440	a single step microbial platform	409:440	a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products	409:589	Consolidated bioprocessing (CBP) by the cellulolytic thermophile Clostridium thermocellum offers a single step microbial platform for production of biofuels and biochemicals via simultaneous solubilization of carbohydrates from lignocellulosic biomass and conversion to products.
35227303	8	114	theme	solubilization	1578:1591	arg1	types					1557:1561	types	1557:1561	types	1557:1561	These results indicate that C. thermocellum may be selective for the types and/or rate of solubilization of the non-cellulosic wall polymers.
35409192	2	0	theme	little	349:354	arg1	evidence					356:363	little evidence	349:363	little evidence showing the relationship between crocetin intake and the risk of gastrointestinal diseases such as colitis	349:470	However, there is little evidence showing the relationship between crocetin intake and the risk of gastrointestinal diseases such as colitis.
35409192	8	1	theme	colitis	1753:1759	arg1	recovery					1741:1748	recovery	1741:1748	recovery of colitis	1741:1759	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	1	2	theme	blood	252:256	arg1	circulation					258:268	blood circulation	252:268	blood circulation	252:268	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	7	3	from	acid	1550:1553	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	4	theme	7-ketodeoxycholic	1477:1493	arg1	acid					1495:1498	7-ketodeoxycholic acid	1477:1498	7-ketodeoxycholic acid	1477:1498	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	chenodeoxycholate					1556:1572	chenodeoxycholate	1556:1572	chenodeoxycholate	1556:1572	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	acid					1471:1474	12-ketodeoxycholic acid	1452:1474	12-ketodeoxycholic acid	1452:1474	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	acid					1644:1647	sulfolithocholic acid	1627:1647	sulfolithocholic acid	1627:1647	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	acid					1520:1523	3-sulfodeoxycholic acid	1501:1523	3-sulfodeoxycholic acid	1501:1523	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	glycochenodeoxycholate-7-sulfate					1575:1606	glycochenodeoxycholate-7-sulfate	1575:1606	glycochenodeoxycholate-7-sulfate	1575:1606	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	acids					1438:1442	primary and secondary bile acids	1411:1442	primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon	1411:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	glycocholate					1609:1620	glycocholate	1609:1620	glycocholate	1609:1620	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	acid					1495:1498	7-ketodeoxycholic acid	1477:1498	7-ketodeoxycholic acid	1477:1498	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	5	theme	bile	1433:1436	arg1	acid					1550:1553	6-ethylchenodeoxycholic acid	1526:1553	6-ethylchenodeoxycholic acid	1526:1553	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	3	6	theme	barrier	552:558	arg1	function					560:567	intestinal barrier function	541:567	intestinal barrier function	541:567	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	7	7	from	chenodeoxycholate	1556:1572	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	4	8	theme	histological	824:835	arg1	abnormalities					837:849	more serious histological abnormalities	811:849	more serious histological abnormalities	811:849	We found that crocetin intake at 10 mg/kg aggravated colitis in mice, showing increased weight loss and more serious histological abnormalities compared with the DSS group.
35409192	6	9	theme	lower	1136:1140	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	8	10	from	products	1845:1852	arg1	mice					1857:1860	mice	1857:1860	mice	1857:1860	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	6	11	theme	Duncaniella	1229:1239	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	11	theme	Duncaniella	1229:1239	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	1	12	theme	saffron	184:190	arg1	constituents					168:179	the major active constituents	151:179	the major active constituents of saffron (Crocus sativus L.)	151:210	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	5	13	dep	species	992:998	arg1	diversity					1000:1008	diversity	1000:1008	diversity	1000:1008	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	5	13	dep	species	992:998	arg1	species					992:998	species diversity and richness	992:1021	species diversity and richness	992:1021	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	5	13	dep	species	992:998	arg1	richness					1014:1021	richness	1014:1021	richness	1014:1021	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	3	14	theme	microbiota	584:593	arg1	composition					595:605	intestinal microbiota composition	573:605	intestinal microbiota composition	573:605	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	8	15	theme	gut	1800:1802	arg1	composition					1815:1825	gut microbiota composition	1800:1825	gut microbiota composition	1800:1825	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	1	16	theme	Crocus	193:198	arg1	L.					208:209	Crocus sativus L.	193:209	Crocus sativus L.	193:209	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	1	16	theme	Crocus	193:198	arg1	saffron					184:190	saffron	184:190	saffron (Crocus sativus L.)	184:210	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	6	17	theme	Mediterraneibacter	1110:1127	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	17	theme	Mediterraneibacter	1110:1127	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	18	theme	crocetin	1318:1325	arg1	group					1327:1331	the crocetin group	1314:1331	the crocetin group	1314:1331	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	3	19	theme	crocetin	511:518	arg1	effect					501:506	the effect	497:506	the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition	497:605	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	7	20	from	acid	1495:1498	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	1	21	contain	has	218:220	arg1	one					144:146	one	144:146	one	144:146	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	1	21	contain	has	218:220	arg1	constituents					168:179	the major active constituents	151:179	the major active constituents of saffron (Crocus sativus L.)	151:210	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	1	21	contain	has	218:220	arg2	reputation					224:233	a reputation	222:233	a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine	222:328	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	7	22	theme	metabolomic	1345:1355	arg1	analyses					1357:1364	Untargeted metabolomic analyses	1334:1364	Untargeted metabolomic analyses	1334:1364	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	6	23	theme	Coriobacteriaceae	1271:1287	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	23	theme	Coriobacteriaceae	1271:1287	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	1	24	theme	major	155:159	arg1	constituents					168:179	the major active constituents	151:179	the major active constituents of saffron (Crocus sativus L.)	151:210	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	7	25	theme	sulfolithocholic	1627:1642	arg1	acid					1644:1647	sulfolithocholic acid	1627:1647	sulfolithocholic acid	1627:1647	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	5	26	theme	rDNA	888:891	arg1	sequencing					893:902	16s rDNA sequencing	884:902	The 16s rDNA sequencing analysis of the feces samples	880:932	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	7	27	theme	acids	1438:1442	arg1	levels					1401:1406	the levels	1397:1406	the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon	1397:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	5	28	theme	10	964:965	arg1	mg/kg					967:971	mg/kg	967:971	mg/kg	967:971	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	5	29	theme	samples	926:932	arg1	analysis					904:911	The 16s rDNA sequencing analysis	880:911	The 16s rDNA sequencing analysis of the feces samples	880:932	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	2	30	theme	diseases	447:454	arg1	intake					407:412	crocetin intake	398:412	crocetin intake	398:412	However, there is little evidence showing the relationship between crocetin intake and the risk of gastrointestinal diseases such as colitis.
35409192	2	30	theme	diseases	447:454	arg1	risk					422:425	the risk	418:425	the risk of gastrointestinal diseases such as colitis	418:470	However, there is little evidence showing the relationship between crocetin intake and the risk of gastrointestinal diseases such as colitis.
35409192	0	31	theme	Recovery	18:25	arg1	Period					27:32	Recovery Period	18:32	Recovery Period of DSS-Induced Colitis	18:55	Crocetin Prolongs Recovery Period of DSS-Induced Colitis via Altering Intestinal Microbiome and Increasing Intestinal Permeability.
35409192	3	32	theme	sulfate	657:663	arg1	DSS					673:675	DSS	673:675	DSS	673:675	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	3	32	theme	sulfate	657:663	arg1	sodium					665:670	3% dextran sulfate sodium	646:670	3% dextran sulfate sodium (DSS) administration for one week	646:704	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	1	33	theme	traditional	301:311	arg1	medicine					321:328	traditional Chinese medicine	301:328	traditional Chinese medicine	301:328	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	3	34	theme	%	647:647	arg1	DSS					673:675	DSS	673:675	DSS	673:675	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	3	34	theme	%	647:647	arg1	sodium					665:670	3% dextran sulfate sodium	646:670	3% dextran sulfate sodium (DSS) administration for one week	646:704	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	0	35	theme	Colitis	49:55	arg1	Period					27:32	Recovery Period	18:32	Recovery Period of DSS-Induced Colitis	18:55	Crocetin Prolongs Recovery Period of DSS-Induced Colitis via Altering Intestinal Microbiome and Increasing Intestinal Permeability.
35409192	8	36	from	composition	1815:1825	arg1	mice					1857:1860	mice	1857:1860	mice	1857:1860	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	7	37	theme	3-sulfodeoxycholic	1501:1518	arg1	acid					1520:1523	3-sulfodeoxycholic acid	1501:1523	3-sulfodeoxycholic acid	1501:1523	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	6	38	theme	higher	1074:1079	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	9	39	theme	bowel	1950:1954	arg1	disease					1956:1962	inflammatory bowel disease	1937:1962	inflammatory bowel disease	1937:1962	Our findings suggest that patients with gastrointestinal diseases such as inflammatory bowel disease should use crocetin with caution.
35409192	4	40	theme	increased	785:793	arg1	loss					802:805	increased weight loss	785:805	increased weight loss	785:805	We found that crocetin intake at 10 mg/kg aggravated colitis in mice, showing increased weight loss and more serious histological abnormalities compared with the DSS group.
35409192	7	41	from	acid	1520:1523	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	6	42	theme	genus	1059:1063	arg1	level					1065:1069	the genus level	1055:1069	the genus level	1055:1069	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	43	theme	Stoquefichus	1253:1264	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	43	theme	Stoquefichus	1253:1264	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	3	44	theme	intestinal	541:550	arg1	function					560:567	intestinal barrier function	541:567	intestinal barrier function	541:567	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	7	45	theme	12-ketodeoxycholic	1452:1469	arg1	acid					1471:1474	12-ketodeoxycholic acid	1452:1474	12-ketodeoxycholic acid	1452:1474	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	4	46	theme	DSS	869:871	arg1	group					873:877	the DSS group	865:877	the DSS group	865:877	We found that crocetin intake at 10 mg/kg aggravated colitis in mice, showing increased weight loss and more serious histological abnormalities compared with the DSS group.
35409192	5	47	theme	16s	884:886	arg1	sequencing					893:902	16s rDNA sequencing	884:902	The 16s rDNA sequencing analysis of the feces samples	880:932	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	8	48	theme	crocetin	1678:1685	arg1	intake					1687:1692	crocetin intake	1678:1692	crocetin intake	1678:1692	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	1	49	from	stasis	291:296	arg1	medicine					321:328	traditional Chinese medicine	301:328	traditional Chinese medicine	301:328	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	4	50	theme	serious	816:822	arg1	abnormalities					837:849	more serious histological abnormalities	811:849	more serious histological abnormalities	811:849	We found that crocetin intake at 10 mg/kg aggravated colitis in mice, showing increased weight loss and more serious histological abnormalities compared with the DSS group.
35409192	7	51	theme	secondary	1423:1431	arg1	chenodeoxycholate					1556:1572	chenodeoxycholate	1556:1572	chenodeoxycholate	1556:1572	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	acid					1471:1474	12-ketodeoxycholic acid	1452:1474	12-ketodeoxycholic acid	1452:1474	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	acid					1644:1647	sulfolithocholic acid	1627:1647	sulfolithocholic acid	1627:1647	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	acid					1520:1523	3-sulfodeoxycholic acid	1501:1523	3-sulfodeoxycholic acid	1501:1523	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	glycochenodeoxycholate-7-sulfate					1575:1606	glycochenodeoxycholate-7-sulfate	1575:1606	glycochenodeoxycholate-7-sulfate	1575:1606	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	acids					1438:1442	primary and secondary bile acids	1411:1442	primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon	1411:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	glycocholate					1609:1620	glycocholate	1609:1620	glycocholate	1609:1620	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	acid					1495:1498	7-ketodeoxycholic acid	1477:1498	7-ketodeoxycholic acid	1477:1498	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	51	theme	secondary	1423:1431	arg1	acid					1550:1553	6-ethylchenodeoxycholic acid	1526:1553	6-ethylchenodeoxycholic acid	1526:1553	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	3	52	theme	intestinal	573:582	arg1	composition					595:605	intestinal microbiota composition	573:605	intestinal microbiota composition	573:605	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	7	53	theme	primary	1411:1417	arg1	chenodeoxycholate					1556:1572	chenodeoxycholate	1556:1572	chenodeoxycholate	1556:1572	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	acid					1471:1474	12-ketodeoxycholic acid	1452:1474	12-ketodeoxycholic acid	1452:1474	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	acid					1644:1647	sulfolithocholic acid	1627:1647	sulfolithocholic acid	1627:1647	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	acid					1520:1523	3-sulfodeoxycholic acid	1501:1523	3-sulfodeoxycholic acid	1501:1523	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	glycochenodeoxycholate-7-sulfate					1575:1606	glycochenodeoxycholate-7-sulfate	1575:1606	glycochenodeoxycholate-7-sulfate	1575:1606	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	acids					1438:1442	primary and secondary bile acids	1411:1442	primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon	1411:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	glycocholate					1609:1620	glycocholate	1609:1620	glycocholate	1609:1620	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	acid					1495:1498	7-ketodeoxycholic acid	1477:1498	7-ketodeoxycholic acid	1477:1498	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	53	theme	primary	1411:1417	arg1	acid					1550:1553	6-ethylchenodeoxycholic acid	1526:1553	6-ethylchenodeoxycholic acid	1526:1553	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	3	54	from	effect	501:506	arg1	composition					595:605	intestinal microbiota composition	573:605	intestinal microbiota composition	573:605	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	3	54	from	effect	501:506	arg1	regulation					527:536	the regulation	523:536	the regulation of intestinal barrier function	523:567	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	3	55	theme	function	560:567	arg1	regulation					527:536	the regulation	523:536	the regulation of intestinal barrier function	523:567	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	3	55	theme	function	560:567	arg1	composition					595:605	intestinal microbiota composition	573:605	intestinal microbiota composition	573:605	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	1	56	theme	sativus	200:206	arg1	L.					208:209	Crocus sativus L.	193:209	Crocus sativus L.	193:209	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	1	56	theme	sativus	200:206	arg1	saffron					184:190	saffron	184:190	saffron (Crocus sativus L.)	184:210	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	7	57	from	acid	1471:1474	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	1	58	theme	constituents	168:179	arg1	one					144:146	one	144:146	one	144:146	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	1	58	theme	constituents	168:179	arg1	constituents					168:179	the major active constituents	151:179	the major active constituents of saffron (Crocus sativus L.)	151:210	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	5	59	dep	lower	986:990	arg1	diversity					1000:1008	diversity	1000:1008	diversity	1000:1008	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	5	59	dep	lower	986:990	arg1	species					992:998	species diversity and richness	992:1021	species diversity and richness	992:1021	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	5	59	dep	lower	986:990	arg1	richness					1014:1021	richness	1014:1021	richness	1014:1021	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	8	60	theme	microbiota	1804:1813	arg1	composition					1815:1825	gut microbiota composition	1800:1825	gut microbiota composition	1800:1825	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	6	61	theme	Parasutterella	1200:1213	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	61	theme	Parasutterella	1200:1213	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	5	62	theme	sequencing	893:902	arg1	analysis					904:911	The 16s rDNA sequencing analysis	880:911	The 16s rDNA sequencing analysis of the feces samples	880:932	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	8	63	theme	metabolic	1835:1843	arg1	products					1845:1852	its metabolic products	1831:1852	its metabolic products in mice	1831:1860	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	6	64	located	observed	1302:1309	arg2	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	64	located	observed	1302:1309	arg2	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	64	located	observed	1302:1309	arg1	level					1065:1069	the genus level	1055:1069	the genus level	1055:1069	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	64	located	observed	1302:1309	arg1	group					1327:1331	the crocetin group	1314:1331	the crocetin group	1314:1331	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	65	theme	Dubosiella	1171:1180	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	65	theme	Dubosiella	1171:1180	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	0	66	theme	Intestinal	70:79	arg1	Microbiome					81:90	Intestinal Microbiome	70:90	Intestinal Microbiome	70:90	Crocetin Prolongs Recovery Period of DSS-Induced Colitis via Altering Intestinal Microbiome and Increasing Intestinal Permeability.
35409192	7	67	theme	Untargeted	1334:1343	arg1	analyses					1357:1364	Untargeted metabolomic analyses	1334:1364	Untargeted metabolomic analyses	1334:1364	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	1	68	theme	active	161:166	arg1	constituents					168:179	the major active constituents	151:179	the major active constituents of saffron (Crocus sativus L.)	151:210	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	0	69	theme	Intestinal	107:116	arg1	Permeability					118:129	Intestinal Permeability	107:129	Intestinal Permeability	107:129	Crocetin Prolongs Recovery Period of DSS-Induced Colitis via Altering Intestinal Microbiome and Increasing Intestinal Permeability.
35409192	4	70	from	mg/kg	743:747	arg1	intake					730:735	crocetin intake	721:735	crocetin intake at 10 mg/kg	721:747	We found that crocetin intake at 10 mg/kg aggravated colitis in mice, showing increased weight loss and more serious histological abnormalities compared with the DSS group.
35409192	2	71	theme	crocetin	398:405	arg1	intake					407:412	crocetin intake	398:412	crocetin intake	398:412	However, there is little evidence showing the relationship between crocetin intake and the risk of gastrointestinal diseases such as colitis.
35409192	5	72	theme	feces	920:924	arg1	samples					926:932	the feces samples	916:932	the feces samples	916:932	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	4	73	theme	crocetin	721:728	arg1	intake					730:735	crocetin intake	721:735	crocetin intake at 10 mg/kg	721:747	We found that crocetin intake at 10 mg/kg aggravated colitis in mice, showing increased weight loss and more serious histological abnormalities compared with the DSS group.
35409192	1	74	theme	blood	285:289	arg1	stasis					291:296	blood stasis	285:296	blood stasis in traditional Chinese medicine	285:328	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	7	75	from	glycochenodeoxycholate-7-sulfate	1575:1606	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	2	76	theme	gastrointestinal	430:445	arg1	diseases					447:454	gastrointestinal diseases	430:454	gastrointestinal diseases such as colitis	430:470	However, there is little evidence showing the relationship between crocetin intake and the risk of gastrointestinal diseases such as colitis.
35409192	2	76	theme	gastrointestinal	430:445	arg1	colitis					464:470	colitis	464:470	colitis	464:470	However, there is little evidence showing the relationship between crocetin intake and the risk of gastrointestinal diseases such as colitis.
35409192	3	77	theme	dextran	649:655	arg1	DSS					673:675	DSS	673:675	DSS	673:675	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	3	77	theme	dextran	649:655	arg1	sodium					665:670	3% dextran sulfate sodium	646:670	3% dextran sulfate sodium (DSS) administration for one week	646:704	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	0	78	theme	DSS-Induced	37:47	arg1	Colitis					49:55	DSS-Induced Colitis	37:55	DSS-Induced Colitis	37:55	Crocetin Prolongs Recovery Period of DSS-Induced Colitis via Altering Intestinal Microbiome and Increasing Intestinal Permeability.
35409192	7	79	from	glycocholate	1609:1620	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	9	80	theme	gastrointestinal	1903:1918	arg1	disease					1956:1962	inflammatory bowel disease	1937:1962	inflammatory bowel disease	1937:1962	Our findings suggest that patients with gastrointestinal diseases such as inflammatory bowel disease should use crocetin with caution.
35409192	9	80	theme	gastrointestinal	1903:1918	arg1	diseases					1920:1927	gastrointestinal diseases	1903:1927	gastrointestinal diseases such as inflammatory bowel disease	1903:1962	Our findings suggest that patients with gastrointestinal diseases such as inflammatory bowel disease should use crocetin with caution.
35409192	3	81	theme	3	646:646	arg1	%					647:647	%	647:647	%	647:647	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	4	82	theme	weight	795:800	arg1	loss					802:805	increased weight loss	785:805	increased weight loss	785:805	We found that crocetin intake at 10 mg/kg aggravated colitis in mice, showing increased weight loss and more serious histological abnormalities compared with the DSS group.
35409192	1	83	theme	Chinese	313:319	arg1	medicine					321:328	traditional Chinese medicine	301:328	traditional Chinese medicine	301:328	Crocetin is one of the major active constituents of saffron (Crocus sativus L.) which has a reputation for facilitating blood circulation and dispersing blood stasis in traditional Chinese medicine.
35409192	6	84	theme	Akkermansia	1094:1104	arg1	abundance					1081:1089	a higher abundance	1072:1089	a higher abundance of Akkermansia and Mediterraneibacter	1072:1127	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	6	84	theme	Akkermansia	1094:1104	arg1	abundance					1142:1150	a lower abundance	1134:1150	a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002	1134:1295	At the genus level, a higher abundance of Akkermansia and Mediterraneibacter, and a lower abundance of Muribaculaceae, Dubosiella, Paramuribaculum, Parasutterella, Allobaculum, Duncaniella, Candidatus Stoquefichus, and Coriobacteriaceae UCG-002 were observed in the crocetin group.
35409192	8	85	theme	intestinal	1704:1713	arg1	homeostasis					1715:1725	intestinal homeostasis	1704:1725	intestinal homeostasis	1704:1725	In conclusion, crocetin intake disturbed intestinal homeostasis and prolonged recovery of colitis by promoting inflammation and altering gut microbiota composition and its metabolic products in mice.
35409192	7	86	from	acid	1644:1647	arg1	colon					1656:1660	the colon	1652:1660	the colon	1652:1660	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	7	87	theme	6-ethylchenodeoxycholic	1526:1548	arg1	acid					1550:1553	6-ethylchenodeoxycholic acid	1526:1553	6-ethylchenodeoxycholic acid	1526:1553	Untargeted metabolomic analyses revealed that crocetin reduced the levels of primary and secondary bile acids such as 12-ketodeoxycholic acid, 7-ketodeoxycholic acid, 3-sulfodeoxycholic acid, 6-ethylchenodeoxycholic acid, chenodeoxycholate, glycochenodeoxycholate-7-sulfate, glycocholate, and sulfolithocholic acid in the colon.
35409192	5	88	theme	mg/kg	967:971	arg1	crocetin					973:980	10 mg/kg crocetin	964:980	10 mg/kg crocetin	964:980	The 16s rDNA sequencing analysis of the feces samples showed that mice treated with 10 mg/kg crocetin had lower species diversity and richness than those treated with DSS.
35409192	9	89	theme	inflammatory	1937:1948	arg1	disease					1956:1962	inflammatory bowel disease	1937:1962	inflammatory bowel disease	1937:1962	Our findings suggest that patients with gastrointestinal diseases such as inflammatory bowel disease should use crocetin with caution.
35409192	3	90	theme	sodium	665:670	arg1	administration					678:691	3% dextran sulfate sodium (DSS) administration	646:691	3% dextran sulfate sodium (DSS) administration for one week	646:704	In order to investigate the effect of crocetin on the regulation of intestinal barrier function and intestinal microbiota composition, mice were treated with crocetin after 3% dextran sulfate sodium (DSS) administration for one week.
35409192	9	91	with	patients	1889:1896	arg1	disease					1956:1962	inflammatory bowel disease	1937:1962	inflammatory bowel disease	1937:1962	Our findings suggest that patients with gastrointestinal diseases such as inflammatory bowel disease should use crocetin with caution.
35409192	9	91	with	patients	1889:1896	arg1	diseases					1920:1927	gastrointestinal diseases	1903:1927	gastrointestinal diseases such as inflammatory bowel disease	1903:1962	Our findings suggest that patients with gastrointestinal diseases such as inflammatory bowel disease should use crocetin with caution.
37065156	11	0	from	microbiota	1846:1855	arg1	groups					1894:1899	different economic-use groups	1871:1899	different economic-use groups	1871:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	3	1	theme	microbiota	491:500	arg1	structures					471:480	community structures	461:480	community structures	461:480	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	3	1	theme	microbiota	491:500	arg1	compositions					444:455	compositions	444:455	compositions	444:455	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	10	2	theme	dual-purpose	1751:1762	arg1	ducks					1769:1773	dual-purpose type ducks	1751:1773	dual-purpose type ducks	1751:1773	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	8	3	theme	gut	1216:1218	arg1	microbiota					1220:1229	the gut microbiota	1212:1229	the gut microbiota	1212:1229	Function prediction of the gut microbiota indicated significant differences between the two groups.
37065156	7	4	theme	LEfSe	1156:1160	arg1	analysis					1162:1169	LEfSe analysis	1156:1169	LEfSe analysis	1156:1169	The biomarkers associated with the egg and dual-purpose type ducks were identified using LEfSe analysis and IndVal index.
37065156	10	5	theme	secondary	1615:1623	arg1	metabolites					1625:1635	secondary metabolites	1615:1635	secondary metabolites	1615:1635	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	1	6	theme	egg	183:185	arg1	ducks					209:213	egg and dual-purpose type ducks	183:213	ducks	209:213	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	9	7	from	ducks	1505:1509	arg1	abundant					1484:1491	abundant	1484:1491	abundant	1484:1491	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	7	from	ducks	1505:1509	arg1	functions					1293:1301	The functions	1289:1301	The functions of environmental information processing	1289:1341	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	10	8	theme	acids	1605:1609	arg1	metabolism					1662:1671	metabolism	1662:1671	metabolism	1662:1671	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	8	theme	acids	1605:1609	arg1	biosynthesis					1583:1594	biosynthesis	1583:1594	biosynthesis of amino acids and secondary metabolites	1583:1635	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	8	theme	acids	1605:1609	arg1	resistance					1709:1718	insulin resistance	1701:1718	insulin resistance	1701:1718	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	8	theme	acids	1605:1609	arg1	processing					1548:1557	the genetic information processing	1524:1557	the genetic information processing	1524:1557	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	8	theme	acids	1605:1609	arg1	biosynthesis					1645:1656	glycan biosynthesis	1638:1656	glycan biosynthesis	1638:1656	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	8	theme	acids	1605:1609	arg1	elongation					1685:1694	fatty acid elongation	1674:1694	fatty acid elongation	1674:1694	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	8	theme	acids	1605:1609	arg1	metabolism					1571:1580	nucleotide metabolism	1560:1580	nucleotide metabolism	1560:1580	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	2	9	theme	important	279:287	arg1	role					289:292	an important role	276:292	an important role	276:292	The gut microbiota of ducks plays an important role in their metabolism, immune regulation, and health maintenance.
37065156	1	10	theme	dual-purpose	191:202	arg1	ducks					209:213	egg and dual-purpose type ducks	183:213	ducks	209:213	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	8	11	theme	Function	1189:1196	arg1	prediction					1198:1207	Function prediction	1189:1207	Function prediction of the gut microbiota	1189:1229	Function prediction of the gut microbiota indicated significant differences between the two groups.
37065156	11	12	theme	ducks	1860:1864	arg1	microbiota					1846:1855	the gut microbiota	1838:1855	the gut microbiota of ducks from different economic-use groups	1838:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	9	13	theme	egg	1496:1498	arg1	ducks					1505:1509	egg type ducks	1496:1509	egg type ducks	1496:1509	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	11	14	theme	microbiota	1846:1855	arg1	diversity					1825:1833	diversity	1825:1833	diversity	1825:1833	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	11	14	theme	microbiota	1846:1855	arg1	structure					1811:1819	structure	1811:1819	structure	1811:1819	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	3	15	theme	type	561:564	arg1	ducks					566:570	dual-purpose type ducks	548:570	dual-purpose type ducks (four breeds, 73 individuals)	548:600	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	11	16	from	probiotics	1975:1984	arg1	production					1989:1998	production	1989:1998	production	1989:1998	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	4	17	theme	fecal	677:681	arg1	microflora					683:692	fecal microflora	677:692	fecal microflora	677:692	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	4	18	theme	type	701:704	arg1	ducks					706:710	egg type ducks	697:710	egg type ducks	697:710	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	11	19	dep	structure	1811:1819	arg1	the					1807:1809	the	1807:1809	the	1807:1809	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	2	20	theme	gut	246:248	arg1	microbiota					250:259	The gut microbiota	242:259	The gut microbiota of ducks	242:268	The gut microbiota of ducks plays an important role in their metabolism, immune regulation, and health maintenance.
37065156	1	21	theme	agricultural	135:146	arg1	Ducks					115:119	Introduction Ducks	102:119	Introduction Ducks	102:119	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	1	21	theme	agricultural	135:146	arg1	animals					148:154	important agricultural animals	125:154	important agricultural animals	125:154	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	6	22	theme	Romboutsia	984:993	arg1	abundance					915:923	the abundance	911:923	the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter	911:1012	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	6	22	theme	Romboutsia	984:993	arg1	different					1033:1041	different	1033:1041	different	1033:1041	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	11	23	theme	related	1967:1973	arg1	probiotics					1975:1984	related probiotics	1967:1984	related probiotics in production	1967:1998	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	11	24	theme	duck	1941:1944	arg1	performance					1946:1956	duck performance	1941:1956	duck performance	1941:1956	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	5	25	theme	microbial	828:836	arg1	richness					848:855	the fecal microbial community richness	818:855	the fecal microbial community richness	818:855	In contrast, there is no significant difference in the fecal microbial community richness between the two groups.
37065156	6	26	theme	Lactobacillus	969:981	arg1	abundance					915:923	the abundance	911:923	the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter	911:1012	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	6	26	theme	Lactobacillus	969:981	arg1	different					1033:1041	different	1033:1041	different	1033:1041	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	6	27	theme	Streptococcaceae	951:966	arg1	abundance					915:923	the abundance	911:923	the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter	911:1012	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	6	27	theme	Streptococcaceae	951:966	arg1	different					1033:1041	different	1033:1041	different	1033:1041	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	1	28	theme	Introduction	102:113	arg1	Ducks					115:119	Introduction Ducks	102:119	Introduction Ducks	102:119	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	1	28	theme	Introduction	102:113	arg1	animals					148:154	important agricultural animals	125:154	important agricultural animals	125:154	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	0	29	theme	type	90:93	arg1	ducks					95:99	egg type ducks	86:99	egg type ducks	86:99	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	4	30	dep	Results	646:652	arg1	diversity					664:672	The alpha diversity	654:672	The alpha diversity of fecal microflora in egg type ducks	654:710	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	4	30	dep	Results	646:652	arg1	higher					730:735	higher	730:735	higher	730:735	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	10	31	theme	acid	1680:1683	arg1	elongation					1685:1694	fatty acid elongation	1674:1694	fatty acid elongation	1674:1694	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	9	32	theme	environmental	1306:1318	arg1	processing					1332:1341	environmental information processing	1306:1341	environmental information processing	1306:1341	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	0	33	from	composition	34:44	arg1	ducks					95:99	egg type ducks	86:99	egg type ducks	86:99	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	0	33	from	composition	34:44	arg1	dual-purpose					69:80	dual-purpose	69:80	dual-purpose	69:80	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	3	34	theme	V4	388:389	arg1	sequencing					414:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	0	35	theme	Microbial	0:8	arg1	diversity					10:18	Microbial diversity	0:18	Microbial diversity	0:18	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	9	36	theme	processing	1332:1341	arg1	metabolism					1417:1426	metabolism	1417:1426	metabolism	1417:1426	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	36	theme	processing	1332:1341	arg1	metabolism					1375:1384	lipid metabolism	1369:1384	lipid metabolism	1369:1384	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	36	theme	processing	1332:1341	arg1	metabolism					1433:1442	metabolism	1433:1442	metabolism of terpenoids and polyketides	1433:1472	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	36	theme	processing	1332:1341	arg1	abundant					1484:1491	abundant	1484:1491	abundant	1484:1491	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	36	theme	processing	1332:1341	arg1	metabolism					1357:1366	carbohydrate metabolism	1344:1366	carbohydrate metabolism	1344:1366	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	36	theme	processing	1332:1341	arg1	biodegradation					1398:1411	xenobiotic biodegradation	1387:1411	xenobiotic biodegradation	1387:1411	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	36	theme	processing	1332:1341	arg1	functions					1293:1301	The functions	1289:1301	The functions of environmental information processing	1289:1341	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	1	37	dep	animals	148:154	arg1	divided					170:176	divided	170:176	can be divided into egg and dual-purpose type ducks according to economic use	163:239	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	10	38	theme	insulin	1701:1707	arg1	resistance					1709:1718	insulin resistance	1701:1718	insulin resistance	1701:1718	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	9	39	theme	carbohydrate	1344:1355	arg1	metabolism					1357:1366	carbohydrate metabolism	1344:1366	carbohydrate metabolism	1344:1366	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	3	40	dep	egg	510:512	arg1	breeds					520:525	five breeds	515:525	five breeds	515:525	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	3	40	dep	egg	510:512	arg1	individuals					531:541	96 individuals	528:541	96 individuals	528:541	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	3	41	theme	16S	379:381	arg1	sequencing					414:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	9	42	theme	terpenoids	1447:1456	arg1	metabolism					1417:1426	metabolism	1417:1426	metabolism	1417:1426	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	42	theme	terpenoids	1447:1456	arg1	metabolism					1375:1384	lipid metabolism	1369:1384	lipid metabolism	1369:1384	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	42	theme	terpenoids	1447:1456	arg1	metabolism					1433:1442	metabolism	1433:1442	metabolism of terpenoids and polyketides	1433:1472	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	42	theme	terpenoids	1447:1456	arg1	abundant					1484:1491	abundant	1484:1491	abundant	1484:1491	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	42	theme	terpenoids	1447:1456	arg1	metabolism					1357:1366	carbohydrate metabolism	1344:1366	carbohydrate metabolism	1344:1366	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	42	theme	terpenoids	1447:1456	arg1	biodegradation					1398:1411	xenobiotic biodegradation	1387:1411	xenobiotic biodegradation	1387:1411	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	42	theme	terpenoids	1447:1456	arg1	functions					1293:1301	The functions	1289:1301	The functions of environmental information processing	1289:1341	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	3	43	theme	rDNA	383:386	arg1	sequencing					414:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	10	44	theme	information	1536:1546	arg1	processing					1548:1557	the genetic information processing	1524:1557	the genetic information processing	1524:1557	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	7	45	theme	dual-purpose	1110:1121	arg1	ducks					1128:1132	the egg and dual-purpose type ducks	1098:1132	ducks	1128:1132	The biomarkers associated with the egg and dual-purpose type ducks were identified using LEfSe analysis and IndVal index.
37065156	3	46	theme	same	629:632	arg1	conditions					634:643	the same conditions	625:643	the same conditions	625:643	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	11	47	theme	different	1871:1879	arg1	groups					1894:1899	different economic-use groups	1871:1899	different economic-use groups	1871:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	9	48	theme	xenobiotic	1387:1396	arg1	biodegradation					1398:1411	xenobiotic biodegradation	1387:1411	xenobiotic biodegradation	1387:1411	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	4	49	theme	dual-type	750:758	arg1	ducks					760:764	dual-type ducks	750:764	dual-type ducks	750:764	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	1	50	theme	economic	228:235	arg1	use					237:239	economic use	228:239	economic use	228:239	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	3	51	dep	compositions	444:455	arg1	the					440:442	the	440:442	the	440:442	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	3	52	theme	fecal	485:489	arg1	microbiota					491:500	fecal microbiota	485:500	fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals)	485:600	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	10	53	theme	amino	1599:1603	arg1	acids					1605:1609	amino acids	1599:1609	amino acids	1599:1609	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	2	54	theme	ducks	264:268	arg1	microbiota					250:259	The gut microbiota	242:259	The gut microbiota of ducks	242:268	The gut microbiota of ducks plays an important role in their metabolism, immune regulation, and health maintenance.
37065156	9	55	theme	polyketides	1462:1472	arg1	metabolism					1417:1426	metabolism	1417:1426	metabolism	1417:1426	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	55	theme	polyketides	1462:1472	arg1	metabolism					1375:1384	lipid metabolism	1369:1384	lipid metabolism	1369:1384	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	55	theme	polyketides	1462:1472	arg1	metabolism					1433:1442	metabolism	1433:1442	metabolism of terpenoids and polyketides	1433:1472	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	55	theme	polyketides	1462:1472	arg1	abundant					1484:1491	abundant	1484:1491	abundant	1484:1491	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	55	theme	polyketides	1462:1472	arg1	metabolism					1357:1366	carbohydrate metabolism	1344:1366	carbohydrate metabolism	1344:1366	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	55	theme	polyketides	1462:1472	arg1	biodegradation					1398:1411	xenobiotic biodegradation	1387:1411	xenobiotic biodegradation	1387:1411	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	9	55	theme	polyketides	1462:1472	arg1	functions					1293:1301	The functions	1289:1301	The functions of environmental information processing	1289:1341	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	5	56	theme	significant	792:802	arg1	difference					804:813	no significant difference	789:813	no significant difference in the fecal microbial community richness between the two groups	789:878	In contrast, there is no significant difference in the fecal microbial community richness between the two groups.
37065156	0	57	theme	fecal	49:53	arg1	microbiota					55:64	fecal microbiota	49:64	fecal microbiota	49:64	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	10	58	theme	metabolites	1625:1635	arg1	metabolism					1662:1671	metabolism	1662:1671	metabolism	1662:1671	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	58	theme	metabolites	1625:1635	arg1	biosynthesis					1583:1594	biosynthesis	1583:1594	biosynthesis of amino acids and secondary metabolites	1583:1635	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	58	theme	metabolites	1625:1635	arg1	resistance					1709:1718	insulin resistance	1701:1718	insulin resistance	1701:1718	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	58	theme	metabolites	1625:1635	arg1	processing					1548:1557	the genetic information processing	1524:1557	the genetic information processing	1524:1557	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	58	theme	metabolites	1625:1635	arg1	biosynthesis					1645:1656	glycan biosynthesis	1638:1656	glycan biosynthesis	1638:1656	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	58	theme	metabolites	1625:1635	arg1	elongation					1685:1694	fatty acid elongation	1674:1694	fatty acid elongation	1674:1694	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	58	theme	metabolites	1625:1635	arg1	metabolism					1571:1580	nucleotide metabolism	1560:1580	nucleotide metabolism	1560:1580	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	3	59	dep	ducks	566:570	arg1	breeds					578:583	four breeds	573:583	four breeds	573:583	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	3	59	dep	ducks	566:570	arg1	individuals					589:599	73 individuals	586:599	73 individuals	586:599	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	8	60	theme	microbiota	1220:1229	arg1	prediction					1198:1207	Function prediction	1189:1207	Function prediction of the gut microbiota	1189:1229	Function prediction of the gut microbiota indicated significant differences between the two groups.
37065156	11	61	theme	gut	1842:1844	arg1	microbiota					1846:1855	the gut microbiota	1838:1855	the gut microbiota of ducks from different economic-use groups	1838:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	8	62	theme	significant	1241:1251	arg1	differences					1253:1263	significant differences	1241:1263	significant differences between the two groups	1241:1286	Function prediction of the gut microbiota indicated significant differences between the two groups.
37065156	11	63	dep	Discussion	1776:1785	arg1	provides					1906:1913	provides	1906:1913	provides a reference for improving duck performance by using related probiotics in production	1906:1998	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	11	63	dep	Discussion	1776:1785	arg1	explored					1798:1805	explored	1798:1805	explored the structure and diversity of the gut microbiota of ducks from different economic-use groups	1798:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	1	64	theme	type	204:207	arg1	ducks					209:213	egg and dual-purpose type ducks	183:213	ducks	209:213	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	10	65	theme	glycan	1638:1643	arg1	biosynthesis					1645:1656	glycan biosynthesis	1638:1656	glycan biosynthesis	1638:1656	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	3	66	theme	dual-purpose	548:559	arg1	ducks					566:570	dual-purpose type ducks	548:570	dual-purpose type ducks (four breeds, 73 individuals)	548:600	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	9	67	theme	type	1500:1503	arg1	ducks					1505:1509	egg type ducks	1496:1509	egg type ducks	1496:1509	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	6	68	theme	MetaStat	881:888	arg1	analysis					890:897	MetaStat analysis	881:897	MetaStat analysis	881:897	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	4	69	theme	alpha	658:662	arg1	diversity					664:672	The alpha diversity	654:672	The alpha diversity of fecal microflora in egg type ducks	654:710	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	4	69	theme	alpha	658:662	arg1	higher					730:735	higher	730:735	higher	730:735	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	1	70	theme	important	125:133	arg1	Ducks					115:119	Introduction Ducks	102:119	Introduction Ducks	102:119	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	1	70	theme	important	125:133	arg1	animals					148:154	important agricultural animals	125:154	important agricultural animals	125:154	Introduction Ducks are important agricultural animals, which can be divided into egg and dual-purpose type ducks according to economic use.
37065156	5	71	theme	fecal	822:826	arg1	richness					848:855	the fecal microbial community richness	818:855	the fecal microbial community richness	818:855	In contrast, there is no significant difference in the fecal microbial community richness between the two groups.
37065156	0	72	theme	egg	86:88	arg1	ducks					95:99	egg type ducks	86:99	egg type ducks	86:99	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	5	73	from	difference	804:813	arg1	richness					848:855	the fecal microbial community richness	818:855	the fecal microbial community richness	818:855	In contrast, there is no significant difference in the fecal microbial community richness between the two groups.
37065156	5	74	theme	community	838:846	arg1	richness					848:855	the fecal microbial community richness	818:855	the fecal microbial community richness	818:855	In contrast, there is no significant difference in the fecal microbial community richness between the two groups.
37065156	0	75	from	diversity	10:18	arg1	ducks					95:99	egg type ducks	86:99	egg type ducks	86:99	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	0	75	from	diversity	10:18	arg1	dual-purpose					69:80	dual-purpose	69:80	dual-purpose	69:80	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	3	76	dep	Methods	358:364	arg1	use					375:377	use	375:377	use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions	375:643	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	7	77	theme	IndVal	1175:1180	arg1	index					1182:1186	IndVal index	1175:1186	IndVal index	1175:1186	The biomarkers associated with the egg and dual-purpose type ducks were identified using LEfSe analysis and IndVal index.
37065156	11	78	theme	economic-use	1881:1892	arg1	groups					1894:1899	different economic-use groups	1871:1899	different economic-use groups	1871:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	10	79	theme	fatty	1674:1678	arg1	elongation					1685:1694	fatty acid elongation	1674:1694	fatty acid elongation	1674:1694	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	9	80	theme	information	1320:1330	arg1	processing					1332:1341	environmental information processing	1306:1341	environmental information processing	1306:1341	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	11	81	from	groups	1894:1899	arg1	ducks					1860:1864	ducks	1860:1864	ducks from different economic-use groups	1860:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	11	81	from	groups	1894:1899	arg1	microbiota					1846:1855	the gut microbiota	1838:1855	the gut microbiota of ducks from different economic-use groups	1838:1899	Discussion This study explored the structure and diversity of the gut microbiota of ducks from different economic-use groups, and provides a reference for improving duck performance by using related probiotics in production.
37065156	4	82	theme	microflora	683:692	arg1	diversity					664:672	The alpha diversity	654:672	The alpha diversity of fecal microflora in egg type ducks	654:710	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	4	82	theme	microflora	683:692	arg1	higher					730:735	higher	730:735	higher	730:735	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	3	83	theme	hypervariable	391:403	arg1	sequencing					414:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	0	84	theme	community	24:32	arg1	composition					34:44	community composition	24:44	community composition	24:44	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	4	85	theme	egg	697:699	arg1	ducks					706:710	egg type ducks	697:710	egg type ducks	697:710	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	7	86	theme	type	1123:1126	arg1	ducks					1128:1132	the egg and dual-purpose type ducks	1098:1132	ducks	1128:1132	The biomarkers associated with the egg and dual-purpose type ducks were identified using LEfSe analysis and IndVal index.
37065156	6	87	theme	Campylobacter	1000:1012	arg1	abundance					915:923	the abundance	911:923	the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter	911:1012	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	6	87	theme	Campylobacter	1000:1012	arg1	different					1033:1041	different	1033:1041	different	1033:1041	MetaStat analysis showed that the abundance of Peptostreptococcaceae, Streptococcaceae, Lactobacillus, Romboutsia, and Campylobacter were significantly different between the two groups.
37065156	3	88	theme	amplicon	405:412	arg1	sequencing					414:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	16S rDNA V4 hypervariable amplicon sequencing	379:423	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	0	89	theme	microbiota	55:64	arg1	diversity					10:18	Microbial diversity	0:18	Microbial diversity	0:18	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	0	89	theme	microbiota	55:64	arg1	composition					34:44	community composition	24:44	community composition	24:44	Microbial diversity and community composition of fecal microbiota in dual-purpose and egg type ducks.
37065156	9	90	theme	lipid	1369:1373	arg1	metabolism					1375:1384	lipid metabolism	1369:1384	lipid metabolism	1369:1384	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	2	91	theme	health	338:343	arg1	maintenance					345:355	health maintenance	338:355	health maintenance	338:355	The gut microbiota of ducks plays an important role in their metabolism, immune regulation, and health maintenance.
37065156	10	92	theme	genetic	1528:1534	arg1	processing					1548:1557	the genetic information processing	1524:1557	the genetic information processing	1524:1557	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	2	93	theme	immune	315:320	arg1	regulation					322:331	immune regulation	315:331	immune regulation	315:331	The gut microbiota of ducks plays an important role in their metabolism, immune regulation, and health maintenance.
37065156	9	94	from	abundant	1484:1491	arg1	ducks					1505:1509	egg type ducks	1496:1509	egg type ducks	1496:1509	The functions of environmental information processing, carbohydrate metabolism, lipid metabolism, xenobiotic biodegradation and metabolism, and metabolism of terpenoids and polyketides were more abundant in egg type ducks.
37065156	3	95	theme	community	461:469	arg1	structures					471:480	community structures	461:480	community structures	461:480	Methods Here, we use 16S rDNA V4 hypervariable amplicon sequencing to investigate the compositions and community structures of fecal microbiota between egg (five breeds, 96 individuals) and dual-purpose type ducks (four breeds, 73 individuals) that were reared under the same conditions.
37065156	4	96	from	diversity	664:672	arg1	ducks					706:710	egg type ducks	697:710	egg type ducks	697:710	Results The alpha diversity of fecal microflora in egg type ducks was significantly higher than that in dual-type ducks.
37065156	10	97	theme	nucleotide	1560:1569	arg1	metabolism					1571:1580	nucleotide metabolism	1560:1580	nucleotide metabolism	1560:1580	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
37065156	10	98	theme	type	1764:1767	arg1	ducks					1769:1773	dual-purpose type ducks	1751:1773	dual-purpose type ducks	1751:1773	Conversely, the genetic information processing, nucleotide metabolism, biosynthesis of amino acids and secondary metabolites, glycan biosynthesis and metabolism, fatty acid elongation, and insulin resistance were significantly enriched in dual-purpose type ducks.
36484340	4	0	theme	Castanopsis	491:501	arg1	CHE					518:520	CHE	518:520	CHE	518:520	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	4	0	theme	Castanopsis	491:501	arg1	extract					509:515	the Castanopsis honey extract	487:515	the Castanopsis honey extract (CHE)	487:521	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	1	1	theme	nectar	164:169	arg1	source					171:176	an important nectar source	151:176	an important nectar source	151:176	Castanopsis is diffusely spread in tropical and subtropical regions and is an important nectar source plant in China.
36484340	7	2	theme	64.81	842:846	arg1	kg-1					851:854	117.58 ± 64.81 mg kg-1	833:854	117.58 ± 64.81 mg kg-1	833:854	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	2	theme	64.81	842:846	arg1	phenylethylamine					815:830	phenylethylamine	815:830	phenylethylamine (117.58 ± 64.81 mg kg-1)	815:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	4	3	theme	inflammatory	561:572	arg1	model					574:578	the RAW 264.7 cell inflammatory model	542:578	the RAW 264.7 cell inflammatory model	542:578	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	7	4	theme	CH	897:898	arg1	content					804:810	The high content	795:810	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1)	795:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	4	theme	CH	897:898	arg1	marker					887:892	a potential marker	875:892	a potential marker of CH	875:898	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	9	5	theme	further	1382:1388	arg1	development					1416:1426	further economic and agricultural development	1382:1426	further economic and agricultural development	1382:1426	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	4	6	theme	cell	556:559	arg1	model					574:578	the RAW 264.7 cell inflammatory model	542:578	the RAW 264.7 cell inflammatory model	542:578	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	4	7	theme	extract	509:515	arg1	effects					476:482	the anti-inflammatory effects	454:482	the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model	454:578	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	8	8	theme	anti-inflammatory	968:984	arg1	activities					986:995	the anti-inflammatory activities	964:995	the anti-inflammatory activities of CHE	964:1002	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	9	9	theme	CH	1447:1448	arg1	application					1432:1442	application	1432:1442	application	1432:1442	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	9	9	theme	CH	1447:1448	arg1	development					1416:1426	further economic and agricultural development	1382:1426	further economic and agricultural development	1382:1426	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	7	10	theme	117.58	833:838	arg1	kg-1					851:854	117.58 ± 64.81 mg kg-1	833:854	117.58 ± 64.81 mg kg-1	833:854	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	10	theme	117.58	833:838	arg1	phenylethylamine					815:830	phenylethylamine	815:830	phenylethylamine (117.58 ± 64.81 mg kg-1)	815:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	8	11	theme	CHE	1000:1002	arg1	activities					986:995	the anti-inflammatory activities	964:995	the anti-inflammatory activities of CHE	964:1002	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	9	12	theme	quality	1319:1325	arg1	level					1310:1314	a high level	1303:1314	a high level of quality	1303:1325	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	4	13	theme	anti-inflammatory	458:474	arg1	effects					476:482	the anti-inflammatory effects	454:482	the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model	454:578	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	5	14	theme	high	604:607	arg1	level					609:613	a high level	602:613	a high level of quality	602:624	The results revealed a high level of quality in CH based on the quality standards.
36484340	4	15	theme	antioxidant	387:397	arg1	capacity					399:406	antioxidant capacity	387:406	antioxidant capacity of CH	387:412	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	7	16	theme	phenylethylamine	815:830	arg1	content					804:810	The high content	795:810	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1)	795:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	16	theme	phenylethylamine	815:830	arg1	marker					887:892	a potential marker	875:892	a potential marker of CH	875:898	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	9	17	theme	high	1305:1308	arg1	level					1310:1314	a high level	1303:1314	a high level of quality	1303:1325	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	8	18	theme	evident	928:934	arg1	activities					948:957	evident antioxidant activities	928:957	evident antioxidant activities	928:957	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	8	19	theme	tumor	1088:1092	arg1	TNF-α					1117:1121	TNF-α	1117:1121	TNF-α	1117:1121	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	8	19	theme	tumor	1088:1092	arg1	alpha					1110:1114	tumor necrosis factor alpha	1088:1114	tumor necrosis factor alpha (TNF-α)	1088:1122	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	7	20	theme	high	799:802	arg1	content					804:810	The high content	795:810	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1)	795:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	20	theme	high	799:802	arg1	marker					887:892	a potential marker	875:892	a potential marker of CH	875:898	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	8	21	theme	necrosis	1094:1101	arg1	TNF-α					1117:1121	TNF-α	1117:1121	TNF-α	1117:1121	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	8	21	theme	necrosis	1094:1101	arg1	alpha					1110:1114	tumor necrosis factor alpha	1088:1114	tumor necrosis factor alpha (TNF-α)	1088:1122	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	7	22	theme	potential	877:885	arg1	content					804:810	The high content	795:810	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1)	795:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	22	theme	potential	877:885	arg1	marker					887:892	a potential marker	875:892	a potential marker of CH	875:898	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	9	23	dep	development	1416:1426	arg1	the					1378:1380	the	1378:1380	the	1378:1380	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	9	24	theme	strong	1338:1343	arg1	evidence					1358:1365	strong experimental evidence	1338:1365	strong experimental evidence	1338:1365	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	0	25	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and anti-inflammatory activities of Castanopsis honey.
36484340	5	26	theme	quality	618:624	arg1	level					609:613	a high level	602:613	a high level of quality	602:624	The results revealed a high level of quality in CH based on the quality standards.
36484340	1	27	dep	source	171:176	arg1	plant					178:182	plant	178:182	plant in China	178:191	Castanopsis is diffusely spread in tropical and subtropical regions and is an important nectar source plant in China.
36484340	9	28	theme	experimental	1345:1356	arg1	evidence					1358:1365	strong experimental evidence	1338:1365	strong experimental evidence	1338:1365	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	0	29	theme	anti-inflammatory	25:41	arg1	activities					43:52	anti-inflammatory activities	25:52	anti-inflammatory activities	25:52	Chemical composition and anti-inflammatory activities of Castanopsis honey.
36484340	4	30	theme	chemical	361:368	arg1	composition					370:380	chemical composition	361:380	chemical composition	361:380	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	1	31	theme	tropical	111:118	arg1	regions					136:142	tropical and subtropical regions	111:142	tropical and subtropical regions	111:142	Castanopsis is diffusely spread in tropical and subtropical regions and is an important nectar source plant in China.
36484340	8	32	theme	antioxidant	936:946	arg1	activities					948:957	evident antioxidant activities	928:957	evident antioxidant activities	928:957	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	8	33	theme	factor	1103:1108	arg1	TNF-α					1117:1121	TNF-α	1117:1121	TNF-α	1117:1121	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	8	33	theme	factor	1103:1108	arg1	alpha					1110:1114	tumor necrosis factor alpha	1088:1114	tumor necrosis factor alpha (TNF-α)	1088:1122	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	9	34	contain	has	1235:1237	arg1	CH					1232:1233	CH	1232:1233	CH	1232:1233	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	9	34	contain	has	1235:1237	arg2	properties					1261:1270	sound physicochemical properties	1239:1270	sound physicochemical properties	1239:1270	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	9	34	contain	has	1235:1237	arg2	activities					1287:1296	biological activities	1276:1296	biological activities	1276:1296	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	8	35	theme	interleukin-10	1151:1164	arg1	content					1140:1146	the content	1136:1146	the content of interleukin-10 (IL-10)	1136:1172	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	4	36	theme	RAW	546:548	arg1	model					574:578	the RAW 264.7 cell inflammatory model	542:578	the RAW 264.7 cell inflammatory model	542:578	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	9	37	theme	sound	1239:1243	arg1	properties					1261:1270	sound physicochemical properties	1239:1270	sound physicochemical properties	1239:1270	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	8	38	theme	alpha	1110:1114	arg1	content					1077:1083	the content	1073:1083	the content of tumor necrosis factor alpha (TNF-α)	1073:1122	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	6	39	theme	high	714:717	arg1	compounds					728:736	5 high response compounds	712:736	5 high response compounds	712:736	Among a total of 84 compounds identified in CH, 5 high response compounds and 29 phenols were further quantified by UPLC-Q/TOF-MS.
36484340	0	40	theme	honey	69:73	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and anti-inflammatory activities of Castanopsis honey.
36484340	0	40	theme	honey	69:73	arg1	activities					43:52	anti-inflammatory activities	25:52	anti-inflammatory activities	25:52	Chemical composition and anti-inflammatory activities of Castanopsis honey.
36484340	9	41	theme	physicochemical	1245:1259	arg1	properties					1261:1270	sound physicochemical properties	1239:1270	sound physicochemical properties	1239:1270	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	1	42	theme	subtropical	124:134	arg1	regions					136:142	tropical and subtropical regions	111:142	tropical and subtropical regions	111:142	Castanopsis is diffusely spread in tropical and subtropical regions and is an important nectar source plant in China.
36484340	0	43	theme	Castanopsis	57:67	arg1	honey					69:73	Castanopsis honey	57:73	Castanopsis honey	57:73	Chemical composition and anti-inflammatory activities of Castanopsis honey.
36484340	5	44	theme	quality	645:651	arg1	standards					653:661	the quality standards	641:661	the quality standards	641:661	The results revealed a high level of quality in CH based on the quality standards.
36484340	4	45	theme	CH	411:412	arg1	parameters					349:358	the physicochemical parameters	329:358	the physicochemical parameters	329:358	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	4	45	theme	CH	411:412	arg1	composition					370:380	chemical composition	361:380	chemical composition	361:380	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	4	45	theme	CH	411:412	arg1	capacity					399:406	antioxidant capacity	387:406	antioxidant capacity of CH	387:412	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	4	46	theme	physicochemical	333:347	arg1	parameters					349:358	the physicochemical parameters	329:358	the physicochemical parameters	329:358	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	9	47	theme	economic	1390:1397	arg1	development					1416:1426	further economic and agricultural development	1382:1426	further economic and agricultural development	1382:1426	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	6	48	theme	response	719:726	arg1	compounds					728:736	5 high response compounds	712:736	5 high response compounds	712:736	Among a total of 84 compounds identified in CH, 5 high response compounds and 29 phenols were further quantified by UPLC-Q/TOF-MS.
36484340	8	49	theme	oxide	1051:1055	arg1	release					1033:1039	the release	1029:1039	the release of nitric oxide (NO)	1029:1060	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	8	50	theme	NF-κB	1192:1196	arg1	pathway					1198:1204	the NF-κB pathway	1188:1204	the NF-κB pathway	1188:1204	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	6	51	theme	compounds	684:692	arg1	total					672:676	a total	670:676	a total of 84 compounds identified in CH	670:709	Among a total of 84 compounds identified in CH, 5 high response compounds and 29 phenols were further quantified by UPLC-Q/TOF-MS.
36484340	9	52	theme	biological	1276:1285	arg1	activities					1287:1296	biological activities	1276:1296	biological activities	1276:1296	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	8	53	theme	nitric	1044:1049	arg1	NO					1058:1059	NO	1058:1059	NO	1058:1059	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	8	53	theme	nitric	1044:1049	arg1	oxide					1051:1055	nitric oxide	1044:1055	nitric oxide (NO)	1044:1060	Furthermore, the CH showed evident antioxidant activities, and the anti-inflammatory activities of CHE were observed to inhibit the release of nitric oxide (NO) and reduce the content of tumor necrosis factor alpha (TNF-α) and improve the content of interleukin-10 (IL-10) by regulating the NF-κB pathway.
36484340	2	54	theme	Castanopsis	198:208	arg1	CH					217:218	CH	217:218	CH	217:218	The Castanopsis honey (CH) is characterized by its bitter taste.
36484340	2	54	theme	Castanopsis	198:208	arg1	honey					210:214	The Castanopsis honey	194:214	The Castanopsis honey (CH)	194:219	The Castanopsis honey (CH) is characterized by its bitter taste.
36484340	2	55	theme	bitter	245:250	arg1	taste					252:256	its bitter taste	241:256	its bitter taste	241:256	The Castanopsis honey (CH) is characterized by its bitter taste.
36484340	9	56	theme	agricultural	1403:1414	arg1	development					1416:1426	further economic and agricultural development	1382:1426	further economic and agricultural development	1382:1426	Our study indicates that CH has sound physicochemical properties and biological activities with a high level of quality, providing strong experimental evidence to support the further economic and agricultural development and application of CH.
36484340	7	57	theme	mg	848:849	arg1	kg-1					851:854	117.58 ± 64.81 mg kg-1	833:854	117.58 ± 64.81 mg kg-1	833:854	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	57	theme	mg	848:849	arg1	phenylethylamine					815:830	phenylethylamine	815:830	phenylethylamine (117.58 ± 64.81 mg kg-1)	815:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	4	58	theme	honey	503:507	arg1	CHE					518:520	CHE	518:520	CHE	518:520	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	4	58	theme	honey	503:507	arg1	extract					509:515	the Castanopsis honey extract	487:515	the Castanopsis honey extract (CHE)	487:521	In this study, the physicochemical parameters, chemical composition, and antioxidant capacity of CH were comprehensively investigated, with the anti-inflammatory effects of the Castanopsis honey extract (CHE) evaluated based on the RAW 264.7 cell inflammatory model.
36484340	1	59	from	plant	178:182	arg1	China					187:191	China	187:191	China	187:191	Castanopsis is diffusely spread in tropical and subtropical regions and is an important nectar source plant in China.
36484340	1	60	theme	important	154:162	arg1	source					171:176	an important nectar source	151:176	an important nectar source	151:176	Castanopsis is diffusely spread in tropical and subtropical regions and is an important nectar source plant in China.
36484340	7	61	theme	±	840:840	arg1	kg-1					851:854	117.58 ± 64.81 mg kg-1	833:854	117.58 ± 64.81 mg kg-1	833:854	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
36484340	7	61	theme	±	840:840	arg1	phenylethylamine					815:830	phenylethylamine	815:830	phenylethylamine (117.58 ± 64.81 mg kg-1)	815:855	The high content of phenylethylamine (117.58 ± 64.81 mg kg-1) was identified as a potential marker of CH.
35017197	4	0	theme	faecal	844:849	arg1	transfer					862:869	faecal microbiota transfer	844:869	faecal microbiota transfer	844:869	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	1	with	prebiotics	926:935	arg1	biotin					915:920	biotin	915:920	biotin	915:920	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	9	2	theme	vitamins	1764:1771	arg1	production					1737:1746	bacterial production	1727:1746	bacterial production of biotin and B vitamins	1727:1771	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	3	3	theme	host	638:641	arg1	metabolism					643:652	host metabolism	638:652	host metabolism	638:652	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	2	4	theme	human	327:331	arg1	composition					361:371	the human gut microbiome's functional composition	323:371	the human gut microbiome's functional composition	323:371	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	10	5	theme	biotin	1862:1867	arg1	supplementation					1883:1897	biotin and prebiotic supplementation	1862:1897	supplementation	1883:1897	CONCLUSION Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
35017197	4	6	from	prebiotics	926:935	arg1	mice					940:943	mice	940:943	mice	940:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	7	theme	antibiotic	816:825	arg1	animals					835:841	germ-free and antibiotic treated animals	802:841	animals	835:841	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	6	8	theme	severe	1184:1189	arg1	obesity					1191:1197	severe obesity	1184:1197	severe obesity	1184:1197	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	1	9	theme	Gut	170:172	arg1	microbiota					174:183	OBJECTIVES Gut microbiota	159:183	OBJECTIVES Gut microbiota	159:183	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	1	9	theme	Gut	170:172	arg1	component					194:202	a key component	188:202	a key component in obesity and type 2 diabetes	188:233	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	9	10	theme	bacterial	1727:1735	arg1	production					1737:1746	bacterial production	1727:1746	bacterial production of biotin and B vitamins	1727:1771	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	3	11	theme	B7/B8	529:533	arg1	biotin					535:540	B7/B8 biotin	529:540	B7/B8 biotin	529:540	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	6	12	theme	circulating	1155:1165	arg1	levels					1174:1179	suboptimal circulating biotin levels	1144:1179	suboptimal circulating biotin levels in severe obesity	1144:1197	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	0	13	theme	biotin	98:103	arg1	effect					88:93	effect	88:93	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.	0:157	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	3	14	theme	vitamins	516:523	arg1	role					506:509	the role	502:509	the role of B vitamins and B7/B8 biotin for regulation of host metabolic state	502:579	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	5	15	from	deficiency	1000:1009	arg1	transporters					1045:1056	transporters	1045:1056	transporters	1045:1056	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	5	15	from	deficiency	1000:1009	arg1	producers					1031:1039	bacterial biotin producers	1014:1039	bacterial biotin producers	1014:1039	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	1	16	theme	key	190:192	arg1	microbiota					174:183	OBJECTIVES Gut microbiota	159:183	OBJECTIVES Gut microbiota	159:183	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	1	16	theme	key	190:192	arg1	component					194:202	a key component	188:202	a key component in obesity and type 2 diabetes	188:233	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	6	17	theme	adipose	1258:1264	arg1	tissue					1266:1271	human adipose tissue	1252:1271	human adipose tissue	1252:1271	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	11	18	theme	TRIAL	1975:1979	arg1	NUMBER					1994:1999	TRIAL REGISTRATION NUMBER NCT02059538	1975:2011	TRIAL REGISTRATION NUMBER NCT02059538.	1975:2012	TRIAL REGISTRATION NUMBER NCT02059538.
35017197	0	19	theme	prebiotic	109:117	arg1	supplementation					119:133	prebiotic supplementation	109:133	prebiotic supplementation	109:133	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	1	20	from	component	194:202	arg1	obesity					207:213	obesity	207:213	obesity	207:213	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	1	20	from	component	194:202	arg1	diabetes					226:233	type 2 diabetes	219:233	type 2 diabetes	219:233	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	4	21	theme	bariatric	872:880	arg1	surgery					882:888	bariatric surgery	872:888	bariatric surgery	872:888	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	7	22	theme	biotin	1386:1391	arg1	levels					1393:1398	host biotin levels	1381:1398	host biotin levels	1381:1398	In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels.
35017197	8	23	theme	biotin	1500:1505	arg1	producers					1507:1515	bacterial biotin producers	1490:1515	bacterial biotin producers	1490:1515	Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice.
35017197	4	24	with	transfer	862:869	arg1	biotin					915:920	biotin	915:920	biotin	915:920	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	0	25	from	effect	88:93	arg1	metabolism					147:156	improved metabolism	138:156	improved metabolism	138:156	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	6	26	theme	biotin-associated	1225:1241	arg1	genes					1243:1247	biotin-associated genes	1225:1247	biotin-associated genes	1225:1247	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	5	27	dep	RESULTS	946:952	arg1	associated					972:981	associated	972:981	is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes	969:1132	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	2	28	theme	MetaCardis	469:478	arg1	cohort					480:485	the MetaCardis cohort	465:485	the MetaCardis cohort	465:485	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	7	29	theme	microbial	1355:1363	arg1	contribution					1365:1376	the microbial contribution	1351:1376	the microbial contribution to host biotin levels	1351:1398	In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels.
35017197	11	30	theme	REGISTRATION	1981:1992	arg1	NUMBER					1994:1999	TRIAL REGISTRATION NUMBER NCT02059538	1975:2011	TRIAL REGISTRATION NUMBER NCT02059538.	1975:2012	TRIAL REGISTRATION NUMBER NCT02059538.
35017197	0	31	theme	biotin	55:60	arg1	status					62:67	host biotin status	50:67	host biotin status	50:67	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	4	32	from	transfer	862:869	arg1	mice					940:943	mice	940:943	mice	940:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	3	33	theme	metabolic	565:573	arg1	state					575:579	host metabolic state	560:579	host metabolic state	560:579	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	4	34	with	animals	835:841	arg1	biotin					915:920	biotin	915:920	biotin	915:920	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	8	35	theme	host	1530:1533	arg1	biotin					1544:1549	host systemic biotin	1530:1549	host systemic biotin in humans and mice	1530:1568	Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice.
35017197	0	36	from	Impairment	0:9	arg1	obesity					79:85	severe obesity	72:85	severe obesity	72:85	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	5	37	theme	host	1091:1094	arg1	phenotypes					1123:1132	host metabolic and inflammatory phenotypes	1091:1132	host metabolic and inflammatory phenotypes	1091:1132	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	5	38	theme	Severe	954:959	arg1	obesity					961:967	Severe obesity	954:967	Severe obesity	954:967	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	4	39	theme	MetaCardis	739:748	arg1	cohorts					750:756	the MetaCardis cohorts	735:756	the MetaCardis cohorts	735:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	2	40	theme	healthy	376:382	arg1	state					394:398	healthy metabolic state	376:398	healthy metabolic state	376:398	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	9	41	theme	high-fat	1594:1601	arg1	mice					1612:1615	high-fat diet-fed mice	1594:1615	high-fat diet-fed mice	1594:1615	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	9	42	theme	weight	1789:1794	arg1	gain					1796:1799	weight gain	1789:1799	weight gain	1789:1799	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	8	43	from	biotin	1544:1549	arg1	mice					1565:1568	mice	1565:1568	mice	1565:1568	Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice.
35017197	8	43	from	biotin	1544:1549	arg1	humans					1554:1559	humans	1554:1559	humans	1554:1559	Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice.
35017197	4	44	from	analyses	704:711	arg1	cohorts					750:756	the MetaCardis cohorts	735:756	the MetaCardis cohorts	735:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	44	from	analyses	704:711	arg1	subjects					721:728	1545 subjects	716:728	1545 subjects from the MetaCardis cohorts	716:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	2	45	theme	severe	413:418	arg1	states					420:425	the most severe states	404:425	the most severe states of obesity and type 2 diabetes within the MetaCardis cohort	404:485	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	8	46	theme	Bariatric	1401:1409	arg1	surgery					1411:1417	Bariatric surgery	1401:1417	Bariatric surgery	1401:1417	Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice.
35017197	0	47	theme	gut	14:16	arg1	metabolism					35:44	gut microbial biotin metabolism	14:44	gut microbial biotin metabolism	14:44	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	1	48	theme	central	267:273	arg1	mechanisms					240:249	mechanisms	240:249	mechanisms	240:249	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	7	49	theme	microbiota	1315:1324	arg1	depletion					1298:1306	depletion	1298:1306	depletion	1298:1306	In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels.
35017197	7	49	theme	microbiota	1315:1324	arg1	absence					1287:1293	absence	1287:1293	absence	1287:1293	In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels.
35017197	0	50	theme	biotin	28:33	arg1	metabolism					35:44	gut microbial biotin metabolism	14:44	gut microbial biotin metabolism	14:44	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	4	51	theme	germ-free	802:810	arg1	animals					835:841	germ-free and antibiotic treated animals	802:841	animals	835:841	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	5	52	theme	biotin	1024:1029	arg1	producers					1031:1039	bacterial biotin producers	1014:1039	bacterial biotin producers	1014:1039	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	10	53	theme	severe	1959:1964	arg1	obesity					1966:1972	severe obesity	1959:1972	severe obesity	1959:1972	CONCLUSION Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
35017197	3	54	theme	microbial	615:623	arg1	function					625:632	microbial function	615:632	microbial function	615:632	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	4	55	theme	murine	772:777	arg1	surgery					882:888	bariatric surgery	872:888	bariatric surgery	872:888	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	55	theme	murine	772:777	arg1	prebiotics					926:935	prebiotics	926:935	prebiotics in mice	926:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	55	theme	murine	772:777	arg1	animals					835:841	germ-free and antibiotic treated animals	802:841	animals	835:841	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	55	theme	murine	772:777	arg1	experiments					779:789	different murine experiments	762:789	different murine experiments	762:789	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	55	theme	murine	772:777	arg1	transfer					862:869	faecal microbiota transfer	844:869	faecal microbiota transfer	844:869	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	1	56	theme	type	219:222	arg1	diabetes					226:233	type 2 diabetes	219:233	type 2 diabetes	219:233	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	5	57	theme	absolute	991:998	arg1	deficiency					1000:1009	an absolute deficiency	988:1009	an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes	988:1132	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	2	58	theme	gut	333:335	arg1	composition					361:371	the human gut microbiome's functional composition	323:371	the human gut microbiome's functional composition	323:371	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	4	59	theme	microbiota	851:860	arg1	transfer					862:869	faecal microbiota transfer	844:869	faecal microbiota transfer	844:869	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	1	60	theme	OBJECTIVES	159:168	arg1	microbiota					174:183	OBJECTIVES Gut microbiota	159:183	OBJECTIVES Gut microbiota	159:183	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	1	60	theme	OBJECTIVES	159:168	arg1	component					194:202	a key component	188:202	a key component in obesity and type 2 diabetes	188:233	OBJECTIVES Gut microbiota is a key component in obesity and type 2 diabetes, yet mechanisms and metabolites central to this interaction remain unclear.
35017197	9	61	theme	glycaemic	1805:1813	arg1	deterioration					1815:1827	glycaemic deterioration	1805:1827	glycaemic deterioration	1805:1827	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	4	62	theme	treated	827:833	arg1	animals					835:841	germ-free and antibiotic treated animals	802:841	animals	835:841	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	6	63	theme	biotin	1167:1172	arg1	levels					1174:1179	suboptimal circulating biotin levels	1144:1179	suboptimal circulating biotin levels in severe obesity	1144:1197	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	3	64	theme	B	514:514	arg1	vitamins					516:523	B vitamins	514:523	B vitamins	514:523	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	10	65	theme	CONCLUSION	1830:1839	arg1	Strategies					1841:1850	CONCLUSION Strategies	1830:1850	CONCLUSION Strategies combining biotin and prebiotic supplementation	1830:1897	CONCLUSION Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
35017197	9	66	theme	biotin	1751:1756	arg1	production					1737:1746	bacterial production	1727:1746	bacterial production of biotin and B vitamins	1727:1771	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	6	67	theme	suboptimal	1144:1153	arg1	levels					1174:1179	suboptimal circulating biotin levels	1144:1179	suboptimal circulating biotin levels in severe obesity	1144:1197	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	0	68	theme	supplementation	119:133	arg1	effect					88:93	effect	88:93	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.	0:157	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	9	69	theme	production	1737:1746	arg1	potential					1714:1722	the potential	1710:1722	the potential of bacterial production of biotin and B vitamins	1710:1771	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	9	69	theme	production	1737:1746	arg1	diversity					1690:1698	the microbiome diversity	1675:1698	the microbiome diversity	1675:1698	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	10	70	theme	prebiotic	1873:1881	arg1	supplementation					1883:1897	biotin and prebiotic supplementation	1862:1897	supplementation	1883:1897	CONCLUSION Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
35017197	9	71	theme	B	1762:1762	arg1	vitamins					1764:1771	B vitamins	1762:1771	B vitamins	1762:1771	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	8	72	theme	bacterial	1490:1498	arg1	producers					1507:1515	bacterial biotin producers	1490:1515	bacterial biotin producers	1490:1515	Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice.
35017197	6	73	theme	human	1252:1256	arg1	tissue					1266:1271	human adipose tissue	1252:1271	human adipose tissue	1252:1271	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	0	74	theme	improved	138:145	arg1	metabolism					147:156	improved metabolism	138:156	improved metabolism	138:156	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	4	75	from	supplementation	894:908	arg1	mice					940:943	mice	940:943	mice	940:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	3	76	theme	biotin	535:540	arg1	role					506:509	the role	502:509	the role of B vitamins and B7/B8 biotin for regulation of host metabolic state	502:579	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	6	77	theme	genes	1243:1247	arg1	levels					1174:1179	suboptimal circulating biotin levels	1144:1179	suboptimal circulating biotin levels in severe obesity	1144:1197	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	6	77	theme	genes	1243:1247	arg1	expression					1211:1220	altered expression	1203:1220	altered expression of biotin-associated genes in human adipose tissue	1203:1271	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	4	78	from	surgery	882:888	arg1	mice					940:943	mice	940:943	mice	940:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	3	79	theme	state	575:579	arg1	regulation					546:555	regulation	546:555	regulation of host metabolic state	546:579	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	4	80	from	experiments	779:789	arg1	cohorts					750:756	the MetaCardis cohorts	735:756	the MetaCardis cohorts	735:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	80	from	experiments	779:789	arg1	subjects					721:728	1545 subjects	716:728	1545 subjects from the MetaCardis cohorts	716:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	7	81	theme	host	1381:1384	arg1	levels					1393:1398	host biotin levels	1381:1398	host biotin levels	1381:1398	In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels.
35017197	0	82	theme	status	62:67	arg1	Impairment					0:9	Impairment	0:9	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.	0:157	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	3	83	theme	host	560:563	arg1	state					575:579	host metabolic state	560:579	host metabolic state	560:579	We focused on the role of B vitamins and B7/B8 biotin for regulation of host metabolic state, as these vitamins influence both microbial function and host metabolism and inflammation.
35017197	8	84	theme	systemic	1535:1542	arg1	biotin					1544:1549	host systemic biotin	1530:1549	host systemic biotin in humans and mice	1530:1568	Bariatric surgery, which improves metabolism and inflammation, associates with increased bacterial biotin producers and improved host systemic biotin in humans and mice.
35017197	6	85	theme	altered	1203:1209	arg1	expression					1211:1220	altered expression	1203:1220	altered expression of biotin-associated genes in human adipose tissue	1203:1271	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	6	86	from	expression	1211:1220	arg1	tissue					1266:1271	human adipose tissue	1252:1271	human adipose tissue	1252:1271	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	6	86	from	expression	1211:1220	arg1	obesity					1191:1197	severe obesity	1184:1197	severe obesity	1184:1197	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	0	87	theme	severe	72:77	arg1	obesity					79:85	severe obesity	72:85	severe obesity	72:85	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	4	88	from	cohorts	750:756	arg1	subjects					721:728	1545 subjects	716:728	1545 subjects from the MetaCardis cohorts	716:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	88	from	cohorts	750:756	arg1	animals					835:841	germ-free and antibiotic treated animals	802:841	animals	835:841	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	88	from	cohorts	750:756	arg1	experiments					779:789	different murine experiments	762:789	different murine experiments	762:789	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	88	from	cohorts	750:756	arg1	surgery					882:888	bariatric surgery	872:888	bariatric surgery	872:888	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	88	from	cohorts	750:756	arg1	prebiotics					926:935	prebiotics	926:935	prebiotics in mice	926:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	88	from	cohorts	750:756	arg1	analyses					704:711	metagenomic analyses	692:711	metagenomic analyses in 1545 subjects from the MetaCardis cohorts	692:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	88	from	cohorts	750:756	arg1	transfer					862:869	faecal microbiota transfer	844:869	faecal microbiota transfer	844:869	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	89	from	animals	835:841	arg1	mice					940:943	mice	940:943	mice	940:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	2	90	theme	diabetes	449:456	arg1	states					420:425	the most severe states	404:425	the most severe states of obesity and type 2 diabetes within the MetaCardis cohort	404:485	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	2	90	theme	diabetes	449:456	arg1	state					394:398	healthy metabolic state	376:398	healthy metabolic state	376:398	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	5	91	theme	metabolic	1096:1104	arg1	phenotypes					1123:1132	host metabolic and inflammatory phenotypes	1091:1132	host metabolic and inflammatory phenotypes	1091:1132	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	7	92	dep	absence	1287:1293	arg1	the					1283:1285	the	1283:1285	the	1283:1285	In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels.
35017197	5	93	theme	inflammatory	1110:1121	arg1	phenotypes					1123:1132	host metabolic and inflammatory phenotypes	1091:1132	host metabolic and inflammatory phenotypes	1091:1132	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	9	94	theme	diet-fed	1603:1610	arg1	mice					1612:1615	high-fat diet-fed mice	1594:1615	high-fat diet-fed mice	1594:1615	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	4	95	with	supplementation	894:908	arg1	biotin					915:920	biotin	915:920	biotin	915:920	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	10	96	theme	states	1949:1954	arg1	deterioration					1922:1934	the deterioration	1918:1934	the deterioration of metabolic states in severe obesity	1918:1972	CONCLUSION Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
35017197	4	97	theme	metagenomic	692:702	arg1	surgery					882:888	bariatric surgery	872:888	bariatric surgery	872:888	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	97	theme	metagenomic	692:702	arg1	prebiotics					926:935	prebiotics	926:935	prebiotics in mice	926:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	97	theme	metagenomic	692:702	arg1	animals					835:841	germ-free and antibiotic treated animals	802:841	animals	835:841	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	97	theme	metagenomic	692:702	arg1	analyses					704:711	metagenomic analyses	692:711	metagenomic analyses in 1545 subjects from the MetaCardis cohorts	692:756	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	97	theme	metagenomic	692:702	arg1	transfer					862:869	faecal microbiota transfer	844:869	faecal microbiota transfer	844:869	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	2	98	theme	functional	350:359	arg1	composition					361:371	the human gut microbiome's functional composition	323:371	the human gut microbiome's functional composition	323:371	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	0	99	theme	microbial	18:26	arg1	metabolism					35:44	gut microbial biotin metabolism	14:44	gut microbial biotin metabolism	14:44	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	4	100	dep	DESIGN	672:677	arg1	performed					682:690	performed	682:690	performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice	682:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	101	with	surgery	882:888	arg1	biotin					915:920	biotin	915:920	biotin	915:920	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	7	102	theme	gut	1311:1313	arg1	microbiota					1315:1324	gut microbiota	1311:1324	gut microbiota	1311:1324	In mice, the absence or depletion of gut microbiota by antibiotics confirmed the microbial contribution to host biotin levels.
35017197	10	103	theme	metabolic	1939:1947	arg1	states					1949:1954	metabolic states	1939:1954	metabolic states	1939:1954	CONCLUSION Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
35017197	6	104	from	levels	1174:1179	arg1	tissue					1266:1271	human adipose tissue	1252:1271	human adipose tissue	1252:1271	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	6	104	from	levels	1174:1179	arg1	obesity					1191:1197	severe obesity	1184:1197	severe obesity	1184:1197	We found suboptimal circulating biotin levels in severe obesity and altered expression of biotin-associated genes in human adipose tissue.
35017197	0	105	theme	metabolism	35:44	arg1	Impairment					0:9	Impairment	0:9	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.	0:157	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	2	106	theme	type	442:445	arg1	diabetes					449:456	type 2 diabetes	442:456	type 2 diabetes	442:456	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	2	107	theme	metabolic	384:392	arg1	state					394:398	healthy metabolic state	376:398	healthy metabolic state	376:398	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	0	108	theme	host	50:53	arg1	status					62:67	host biotin status	50:67	host biotin status	50:67	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	5	109	theme	bacterial	1014:1022	arg1	producers					1031:1039	bacterial biotin producers	1014:1039	bacterial biotin producers	1014:1039	RESULTS Severe obesity is associated with an absolute deficiency in bacterial biotin producers and transporters, whose abundances correlate with host metabolic and inflammatory phenotypes.
35017197	2	110	theme	obesity	430:436	arg1	states					420:425	the most severe states	404:425	the most severe states of obesity and type 2 diabetes within the MetaCardis cohort	404:485	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	2	110	theme	obesity	430:436	arg1	state					394:398	healthy metabolic state	376:398	healthy metabolic state	376:398	We examined the human gut microbiome's functional composition in healthy metabolic state and the most severe states of obesity and type 2 diabetes within the MetaCardis cohort.
35017197	0	111	dep	Impairment	0:9	arg1	effect					88:93	effect	88:93	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.	0:157	Impairment of gut microbial biotin metabolism and host biotin status in severe obesity: effect of biotin and prebiotic supplementation on improved metabolism.
35017197	10	112	from	deterioration	1922:1934	arg1	obesity					1966:1972	severe obesity	1959:1972	severe obesity	1959:1972	CONCLUSION Strategies combining biotin and prebiotic supplementation could help prevent the deterioration of metabolic states in severe obesity.
35017197	9	113	theme	microbiome	1679:1688	arg1	diversity					1690:1698	the microbiome diversity	1675:1698	the microbiome diversity	1675:1698	Finally, supplementing high-fat diet-fed mice with fructo-oligosaccharides and biotin improves not only the microbiome diversity, but also the potential of bacterial production of biotin and B vitamins, while limiting weight gain and glycaemic deterioration.
35017197	4	114	theme	different	762:770	arg1	surgery					882:888	bariatric surgery	872:888	bariatric surgery	872:888	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	114	theme	different	762:770	arg1	prebiotics					926:935	prebiotics	926:935	prebiotics in mice	926:943	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	114	theme	different	762:770	arg1	animals					835:841	germ-free and antibiotic treated animals	802:841	animals	835:841	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	114	theme	different	762:770	arg1	experiments					779:789	different murine experiments	762:789	different murine experiments	762:789	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
35017197	4	114	theme	different	762:770	arg1	transfer					862:869	faecal microbiota transfer	844:869	faecal microbiota transfer	844:869	DESIGN We performed metagenomic analyses in 1545 subjects from the MetaCardis cohorts and different murine experiments, including germ-free and antibiotic treated animals, faecal microbiota transfer, bariatric surgery and supplementation with biotin and prebiotics in mice.
36224686	10	0	from	end	1426:1428	arg1	spleen					1375:1380	spleen	1375:1380	spleen	1375:1380	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	0	from	end	1426:1428	arg1	samples					1366:1372	the fecal samples	1356:1372	the fecal samples	1356:1372	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	0	from	end	1426:1428	arg1	tissue					1316:1321	The intestinal tissue	1301:1321	The intestinal tissue at the end of the experiment	1301:1350	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	0	from	end	1426:1428	arg1	serum					1386:1390	serum	1386:1390	serum	1386:1390	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	12	1	theme	higher	2064:2069	arg1	Bifidobacteriumwas					2031:2048	Bifidobacteriumwas	2031:2048	Bifidobacteriumwas significantly higher	2031:2069	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	13	2	theme	relative	2520:2527	arg1	abundance					2529:2537	reduced relative abundance	2512:2537	reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05)	2512:2631	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	12	3	theme	organ	2204:2208	arg1	index					2210:2214	the splenic organ index	2192:2214	the splenic organ index	2192:2214	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	3	theme	organ	2204:2208	arg1	higher					2234:2239	higher	2234:2239	higher	2234:2239	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	3	4	from	volume	611:616	arg1	group					510:514	a normal saline (NS) group	489:514	a normal saline (NS) group	489:514	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	3	4	from	volume	611:616	arg1	group					530:534	a TMC3115 group	520:534	a TMC3115 group	520:534	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	7	5	theme	regular	985:991	arg1	feed					993:996	regular feed	985:996	regular feed	985:996	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	10	6	theme	colonic	1500:1506	arg1	inflammation					1508:1519	colonic inflammation	1500:1519	colonic inflammation	1500:1519	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	11	7	theme	TMC3115-DSS	1734:1744	arg1	group					1746:1750	the TMC3115-DSS group	1730:1750	the TMC3115-DSS group	1730:1750	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	1	8	theme	long-term	284:292	arg1	impact					294:299	the long-term impact	280:299	the long-term impact on inflammatory bowel disease	280:329	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	10	9	theme	experiment	1341:1350	arg1	end					1330:1332	the end	1326:1332	the end of the experiment	1326:1350	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	1	10	theme	Bifidobacterium	184:198	arg1	TMC3115					208:214	Bifidobacterium bifidum TMC3115	184:214	Bifidobacterium bifidum TMC3115 in early life	184:228	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	13	11	theme	TMC3115-DSS	2490:2500	arg1	group					2502:2506	the TMC3115-DSS group	2486:2506	the TMC3115-DSS group	2486:2506	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	11	12	theme	crypt	1838:1842	arg1	structures					1844:1853	colonic crypt structures	1830:1853	colonic crypt structures	1830:1853	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	10	13	theme	composition	1526:1536	arg1	scores					1490:1495	the pathology scores	1476:1495	the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines	1476:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	6	14	from	group	765:769	arg1	half					740:743	half	740:743	half of the mice in each group	740:769	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	2	15	theme	Methods	332:338	arg1	mice					365:368	Methods Fourteen pregnant BALB/c mice	332:368	Methods Fourteen pregnant BALB/c mice	332:368	Methods Fourteen pregnant BALB/c mice were purchased and 84 newborn BALB/c mice were subsequently obtained.
36224686	9	16	theme	body	1254:1257	arg1	changes					1264:1270	The weekly body mass changes	1243:1270	The weekly body mass changes of the mice	1243:1282	The weekly body mass changes of the mice were documented.
36224686	14	17	theme	gut	2839:2841	arg1	microbiota					2843:2852	gut microbiota	2839:2852	gut microbiota	2839:2852	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	3	18	theme	oral	547:550	arg1	gavage					552:557	oral gavage normal saline and TMC3115	547:583	gavage	552:557	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	1	19	theme	early	219:223	arg1	life					225:228	early life	219:228	early life	219:228	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	6	20	theme	NS-water	857:864	arg1	group					866:870	NS-water group	857:870	NS-water group	857:870	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	12	21	theme	relative	2009:2016	arg1	abundance					2018:2026	the relative abundance	2005:2026	the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10	2005:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	3	22	theme	newborn	450:456	arg1	mice					458:461	the newborn mice	446:461	the newborn mice	446:461	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	13	23	with	comparison	2414:2423	arg1	those					2430:2434	those	2430:2434	those	2430:2434	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	10	24	theme	microbiota	1551:1560	arg1	concentration					1595:1607	the mass concentration	1586:1607	the mass concentration of serum cytokines	1586:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	24	theme	microbiota	1551:1560	arg1	index					1576:1580	spleen organ index	1563:1580	spleen organ index	1563:1580	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	24	theme	microbiota	1551:1560	arg1	inflammation					1508:1519	colonic inflammation	1500:1519	colonic inflammation	1500:1519	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	24	theme	microbiota	1551:1560	arg1	composition					1526:1536	the composition	1522:1536	the composition of fecal gut microbiota	1522:1560	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	12	25	dep	lower	2175:2179	arg1	P<0.05					2183:2188	P<0.05	2183:2188	P<0.05	2183:2188	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	11	26	dep	lower	1721:1725	arg1	1					1637:1637	1	1637:1637	1	1637:1637	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	13	27	theme	splenic	2644:2650	arg1	index					2658:2662	the splenic organ index	2640:2662	the splenic organ index	2640:2662	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	27	theme	splenic	2644:2650	arg1	higher					2682:2687	higher	2682:2687	higher	2682:2687	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	7	28	theme	ad	1184:1185	arg1	libitum					1187:1193	given pure water ad libitum	1167:1193	given pure water ad libitum	1167:1193	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	28	theme	ad	1184:1185	arg1	groups					1155:1160	the non-modeling groups	1138:1160	the non-modeling groups	1138:1160	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	10	29	theme	fecal	1541:1545	arg1	microbiota					1551:1560	fecal gut microbiota	1541:1560	fecal gut microbiota	1541:1560	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	13	30	from	changes	2730:2736	arg1	IL-6					2741:2744	IL-6	2741:2744	IL-6	2741:2744	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	30	from	changes	2730:2736	arg1	P>0.05					2757:2762	P>0.05	2757:2762	P>0.05	2757:2762	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	30	from	changes	2730:2736	arg1	TNF-α					2749:2753	TNF-α	2749:2753	TNF-α ( P>0.05)	2749:2763	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	12	31	theme	NS	1995:1996	arg1	group					1998:2002	the NS group	1991:2002	the NS group	1991:2002	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	7	32	theme	pure	1173:1176	arg1	libitum					1187:1193	given pure water ad libitum	1167:1193	given pure water ad libitum	1167:1193	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	32	theme	pure	1173:1176	arg1	groups					1155:1160	the non-modeling groups	1138:1160	the non-modeling groups	1138:1160	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	10	33	theme	index	1576:1580	arg1	scores					1490:1495	the pathology scores	1476:1495	the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines	1476:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	6	34	theme	TMC3115-DSS	912:922	arg1	group					924:928	TMC3115-DSS group	912:928	TMC3115-DSS group	912:928	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	12	35	with	comparison	1966:1975	arg1	those					1982:1986	those	1982:1986	those	1982:1986	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	10	36	theme	spleen	1563:1568	arg1	index					1576:1580	spleen organ index	1563:1580	spleen organ index	1563:1580	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	11	37	theme	intact	1918:1923	arg1	structures					1936:1945	more intact epithelial structures	1913:1945	more intact epithelial structures	1913:1945	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	12	38	from	change	2346:2351	arg1	P>0.05					2372:2377	P>0.05	2372:2377	P>0.05	2372:2377	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	38	from	change	2346:2351	arg1	TNF-α					2364:2368	TNF-α	2364:2368	TNF-α ( P>0.05)	2364:2378	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	38	from	change	2346:2351	arg1	IL-6					2356:2359	IL-6	2356:2359	IL-6	2356:2359	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	7	39	theme	sodium	1063:1068	arg1	libitum					1079:1085	3% dextran sulphate sodium (DSS) ad libitum	1043:1085	3% dextran sulphate sodium (DSS) ad libitum	1043:1085	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	1	40	from	effects	167:173	arg1	microbiota					244:253	intestinal microbiota	233:253	intestinal microbiota	233:253	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	1	40	from	effects	167:173	arg1	functions					266:274	immune functions	259:274	immune functions	259:274	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	1	40	from	effects	167:173	arg1	disease					323:329	inflammatory bowel disease	304:329	inflammatory bowel disease	304:329	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	6	41	theme	TMC3115-water	887:899	arg1	group					901:905	TMC3115-water group	887:905	TMC3115-water group	887:905	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	12	42	theme	interleukin	2256:2266	arg1	feces					2078:2082	the feces	2074:2082	the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10	2074:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	7	43	theme	dextran	1046:1052	arg1	sodium					1063:1068	3% dextran sulphate sodium	1043:1068	3% dextran sulphate sodium (DSS) ad libitum	1043:1085	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	43	theme	dextran	1046:1052	arg1	DSS					1071:1073	DSS	1071:1073	DSS	1071:1073	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	14	44	theme	TMC3115	2788:2794	arg1	use					2781:2783	The use	2777:2783	The use of TMC3115 in early life	2777:2808	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	12	45	dep	decreased	2294:2302	arg1	P<0.05					2306:2311	P<0.05	2306:2311	P<0.05	2306:2311	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	45	dep	decreased	2294:2302	arg1	2					1948:1948	2	1948:1948	2	1948:1948	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	11	46	theme	experiment	1658:1667	arg1	end					1647:1649	the end	1643:1649	the end of the experiment	1643:1667	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	0	47	theme	Bowel	122:126	arg1	Disease					128:134	Inflammatory Bowel Disease	109:134	Inflammatory Bowel Disease	109:134	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	14	48	theme	neonatal	2857:2864	arg1	mice					2866:2869	neonatal mice	2857:2869	neonatal mice	2857:2869	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	0	49	theme	[Bifidobacterium	0:15	arg1	TMC3115					25:31	[Bifidobacterium bifidum TMC3115	0:31	[Bifidobacterium bifidum TMC3115	0:31	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	6	50	from	half	740:743	arg1	group					765:769	each group	760:769	each group	760:769	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	11	51	theme	structures	1865:1874	arg1	structures					1936:1945	more intact epithelial structures	1913:1945	more intact epithelial structures	1913:1945	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	51	theme	structures	1865:1874	arg1	disruption					1816:1825	less disruption	1811:1825	less disruption of colonic crypt structures and other structures	1811:1874	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	51	theme	structures	1865:1874	arg1	infiltration					1895:1906	less inflammatory infiltration	1877:1906	less inflammatory infiltration	1877:1906	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	1	52	theme	inflammatory	304:315	arg1	disease					323:329	inflammatory bowel disease	304:329	inflammatory bowel disease	304:329	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	10	53	from	end	1330:1332	arg1	spleen					1375:1380	spleen	1375:1380	spleen	1375:1380	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	53	from	end	1330:1332	arg1	samples					1366:1372	the fecal samples	1356:1372	the fecal samples	1356:1372	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	53	from	end	1330:1332	arg1	tissue					1316:1321	The intestinal tissue	1301:1321	The intestinal tissue at the end of the experiment	1301:1350	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	53	from	end	1330:1332	arg1	serum					1386:1390	serum	1386:1390	serum	1386:1390	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	12	54	theme	abundance	2123:2131	arg1	feces					2078:2082	the feces	2074:2082	the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10	2074:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	10	55	theme	concentration	1595:1607	arg1	scores					1490:1495	the pathology scores	1476:1495	the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines	1476:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	0	56	theme	Early	42:46	arg1	Life					48:51	Early Life	42:51	Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease]	42:135	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	7	57	theme	week	1015:1018	arg1	end					1008:1010	the end	1004:1010	the end of week 6	1004:1020	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	0	58	dep	Intestinal	53:62	arg1	Alleviate					87:95	Alleviate	87:95	to Alleviate Symptoms of Inflammatory Bowel Disease	84:134	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	7	59	theme	enteritis	1115:1123	arg1	model					1125:1129	the enteritis model	1111:1129	the enteritis model	1111:1129	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	0	60	theme	Intestinal	53:62	arg1	Life					48:51	Early Life	42:51	Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease]	42:135	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	3	61	theme	normal	491:496	arg1	NS					506:507	NS	506:507	NS	506:507	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	3	61	theme	normal	491:496	arg1	saline					498:503	normal saline	491:503	a normal saline (NS) group	489:514	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	10	62	theme	mass	1590:1593	arg1	concentration					1595:1607	the mass concentration	1586:1607	the mass concentration of serum cytokines	1586:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	63	theme	intestinal	1305:1314	arg1	tissue					1316:1321	The intestinal tissue	1301:1321	The intestinal tissue at the end of the experiment	1301:1350	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	64	theme	experiment	1437:1446	arg1	end					1426:1428	the end	1422:1428	the end of the experiment	1422:1446	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	1	65	theme	intestinal	233:242	arg1	microbiota					244:253	intestinal microbiota	233:253	intestinal microbiota	233:253	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	6	66	theme	mice	752:755	arg1	half					740:743	half	740:743	half of the mice in each group	740:769	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	13	67	theme	significant	2718:2728	arg1	changes					2730:2736	no significant changes	2715:2736	no significant changes in IL-6 or TNF-α ( P>0.05)	2715:2763	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	68	contain	had	2508:2510	arg2	abundance					2529:2537	reduced relative abundance	2512:2537	reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05)	2512:2631	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	68	contain	had	2508:2510	arg1	group					2502:2506	the TMC3115-DSS group	2486:2506	the TMC3115-DSS group	2486:2506	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	10	69	theme	serum	1612:1616	arg1	cytokines					1618:1626	serum cytokines	1612:1626	serum cytokines	1612:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	70	theme	pathology	1480:1488	arg1	scores					1490:1495	the pathology scores	1476:1495	the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines	1476:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	12	71	theme	splenic	2196:2202	arg1	index					2210:2214	the splenic organ index	2192:2214	the splenic organ index	2192:2214	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	71	theme	splenic	2196:2202	arg1	higher					2234:2239	higher	2234:2239	higher	2234:2239	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	14	72	theme	long-term	2892:2900	arg1	effect					2902:2907	a long-term effect	2890:2907	a long-term effect that alleviates colitis in mice	2890:2939	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	13	73	theme	reduced	2512:2518	arg1	abundance					2529:2537	reduced relative abundance	2512:2537	reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05)	2512:2631	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	11	74	theme	colonic	1830:1836	arg1	structures					1844:1853	colonic crypt structures	1830:1853	colonic crypt structures	1830:1853	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	14	75	dep	Conclusion	2766:2775	arg1	understood					2990:2999	understood	2990:2999	are still not fully understood	2970:2999	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	14	75	dep	Conclusion	2766:2775	arg1	promotes					2810:2817	promotes	2810:2817	promotes	2810:2817	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	7	76	theme	ad	1076:1077	arg1	libitum					1079:1085	3% dextran sulphate sodium (DSS) ad libitum	1043:1085	3% dextran sulphate sodium (DSS) ad libitum	1043:1085	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	10	77	theme	fecal	1360:1364	arg1	samples					1366:1372	the fecal samples	1356:1372	the fecal samples	1356:1372	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	6	78	from	mice	945:948	arg1	group					958:962	each group	953:962	each group	953:962	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	10	79	theme	inflammation	1508:1519	arg1	scores					1490:1495	the pathology scores	1476:1495	the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines	1476:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	6	80	theme	remaining	801:809	arg1	mice					811:814	the remaining mice	797:814	the remaining mice in each group	797:828	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	3	81	theme	TMC3115	522:528	arg1	group					530:534	a TMC3115 group	520:534	a TMC3115 group	520:534	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	11	82	theme	other	1859:1863	arg1	structures					1865:1874	other structures	1859:1874	other structures	1859:1874	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	1	83	from	impact	294:299	arg1	microbiota					244:253	intestinal microbiota	233:253	intestinal microbiota	233:253	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	1	83	from	impact	294:299	arg1	functions					266:274	immune functions	259:274	immune functions	259:274	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	1	83	from	impact	294:299	arg1	disease					323:329	inflammatory bowel disease	304:329	inflammatory bowel disease	304:329	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	9	84	theme	weekly	1247:1252	arg1	changes					1264:1270	The weekly body mass changes	1243:1270	The weekly body mass changes of the mice	1243:1282	The weekly body mass changes of the mice were documented.
36224686	6	85	theme	NS-DSS	873:878	arg1	group					880:884	NS-DSS group	873:884	NS-DSS group	873:884	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	11	86	theme	structures	1844:1853	arg1	structures					1936:1945	more intact epithelial structures	1913:1945	more intact epithelial structures	1913:1945	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	86	theme	structures	1844:1853	arg1	disruption					1816:1825	less disruption	1811:1825	less disruption of colonic crypt structures and other structures	1811:1874	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	86	theme	structures	1844:1853	arg1	infiltration					1895:1906	less inflammatory infiltration	1877:1906	less inflammatory infiltration	1877:1906	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	14	87	theme	early	2799:2803	arg1	life					2805:2808	early life	2799:2808	early life	2799:2808	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	9	88	theme	mass	1259:1262	arg1	changes					1264:1270	The weekly body mass changes	1243:1270	The weekly body mass changes of the mice	1243:1282	The weekly body mass changes of the mice were documented.
36224686	3	89	theme	normal	559:564	arg1	saline					566:571	normal saline	559:571	normal saline	559:571	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	12	90	theme	significant	2334:2344	arg1	change					2346:2351	no significant change	2331:2351	no significant change in IL-6 or TNF-α ( P>0.05)	2331:2378	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	10	91	theme	mice	1399:1402	arg1	spleen					1375:1380	spleen	1375:1380	spleen	1375:1380	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	91	theme	mice	1399:1402	arg1	samples					1366:1372	the fecal samples	1356:1372	the fecal samples	1356:1372	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	91	theme	mice	1399:1402	arg1	tissue					1316:1321	The intestinal tissue	1301:1321	The intestinal tissue at the end of the experiment	1301:1350	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	91	theme	mice	1399:1402	arg1	serum					1386:1390	serum	1386:1390	serum	1386:1390	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	11	92	theme	inflammatory	1674:1685	arg1	score					1697:1701	the inflammatory pathology score	1670:1701	the inflammatory pathology score	1670:1701	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	92	theme	inflammatory	1674:1685	arg1	lower					1721:1725	lower	1721:1725	lower	1721:1725	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	93	theme	Saline-DSS	1778:1787	arg1	group					1789:1793	the Saline-DSS group	1774:1793	the Saline-DSS group ( P<0.05)	1774:1803	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	93	theme	Saline-DSS	1778:1787	arg1	P<0.05					1797:1802	P<0.05	1797:1802	P<0.05	1797:1802	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	12	94	theme	Bifidobacteriumwas	2031:2048	arg1	abundance					2018:2026	the relative abundance	2005:2026	the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10	2005:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	7	95	theme	%	1044:1044	arg1	sodium					1063:1068	3% dextran sulphate sodium	1043:1068	3% dextran sulphate sodium (DSS) ad libitum	1043:1085	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	95	theme	%	1044:1044	arg1	DSS					1071:1073	DSS	1071:1073	DSS	1071:1073	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	10	96	theme	gut	1547:1549	arg1	microbiota					1551:1560	fecal gut microbiota	1541:1560	fecal gut microbiota	1541:1560	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	14	97	theme	microbiota	2843:2852	arg1	construction					2823:2834	the construction	2819:2834	the construction of gut microbiota in neonatal mice	2819:2869	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	9	98	theme	mice	1279:1282	arg1	changes					1264:1270	The weekly body mass changes	1243:1270	The weekly body mass changes of the mice	1243:1282	The weekly body mass changes of the mice were documented.
36224686	13	99	theme	organ	2652:2656	arg1	index					2658:2662	the splenic organ index	2640:2662	the splenic organ index	2640:2662	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	99	theme	organ	2652:2656	arg1	higher					2682:2687	higher	2682:2687	higher	2682:2687	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	100	theme	DSS	2471:2473	arg1	induction					2475:2483	DSS induction	2471:2483	DSS induction	2471:2483	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	14	101	from	colitis	2925:2931	arg1	mice					2936:2939	mice	2936:2939	mice	2936:2939	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	7	102	theme	water	1178:1182	arg1	libitum					1187:1193	given pure water ad libitum	1167:1193	given pure water ad libitum	1167:1193	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	102	theme	water	1178:1182	arg1	groups					1155:1160	the non-modeling groups	1138:1160	the non-modeling groups	1138:1160	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	2	103	theme	BALB/c	358:363	arg1	mice					365:368	Methods Fourteen pregnant BALB/c mice	332:368	Methods Fourteen pregnant BALB/c mice	332:368	Methods Fourteen pregnant BALB/c mice were purchased and 84 newborn BALB/c mice were subsequently obtained.
36224686	10	104	theme	organ	1570:1574	arg1	index					1576:1580	spleen organ index	1563:1580	spleen organ index	1563:1580	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	1	105	from	TMC3115	208:214	arg1	life					225:228	early life	219:228	early life	219:228	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	7	106	theme	given	1167:1171	arg1	libitum					1187:1193	given pure water ad libitum	1167:1193	given pure water ad libitum	1167:1193	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	106	theme	given	1167:1171	arg1	groups					1155:1160	the non-modeling groups	1138:1160	the non-modeling groups	1138:1160	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	13	107	dep	tumefaciens	2573:2583	arg1	feces					2617:2621	the feces	2613:2621	the feces	2613:2621	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	13	107	dep	tumefaciens	2573:2583	arg1	Staphylococcus					2542:2555	Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces	2542:2621	Staphylococcus	2542:2555	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	7	108	theme	sulphate	1054:1061	arg1	sodium					1063:1068	3% dextran sulphate sodium	1043:1068	3% dextran sulphate sodium (DSS) ad libitum	1043:1085	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	108	theme	sulphate	1054:1061	arg1	DSS					1071:1073	DSS	1071:1073	DSS	1071:1073	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	13	109	theme	NS-DSS	2443:2448	arg1	group					2450:2454	the NS-DSS group	2439:2454	the NS-DSS group that undergone DSS induction	2439:2483	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	3	110	dep	gavage	552:557	arg1	saline					566:571	normal saline	559:571	normal saline	559:571	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	11	111	theme	pathology	1687:1695	arg1	score					1697:1701	the inflammatory pathology score	1670:1701	the inflammatory pathology score	1670:1701	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	111	theme	pathology	1687:1695	arg1	lower					1721:1725	lower	1721:1725	lower	1721:1725	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	11	112	theme	epithelial	1925:1934	arg1	structures					1936:1945	more intact epithelial structures	1913:1945	more intact epithelial structures	1913:1945	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	3	113	theme	mL	625:626	arg1	volume					611:616	a daily volume	603:616	a daily volume of 0.2 mL for each mouse	603:641	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	0	114	theme	Inflammatory	109:120	arg1	Disease					128:134	Inflammatory Bowel Disease	109:134	Inflammatory Bowel Disease	109:134	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	3	115	theme	saline	498:503	arg1	group					510:514	a normal saline (NS) group	489:514	a normal saline (NS) group	489:514	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	0	116	theme	Disease	128:134	arg1	Symptoms					97:104	Symptoms	97:104	Symptoms of Inflammatory Bowel Disease	97:134	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	7	117	theme	non-modeling	1142:1153	arg1	libitum					1187:1193	given pure water ad libitum	1167:1193	given pure water ad libitum	1167:1193	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	7	117	theme	non-modeling	1142:1153	arg1	groups					1155:1160	the non-modeling groups	1138:1160	the non-modeling groups	1138:1160	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	6	118	from	mice	752:755	arg1	group					765:769	each group	760:769	each group	760:769	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	13	119	theme	Shigellain	2602:2611	arg1	abundance					2529:2537	reduced relative abundance	2512:2537	reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05)	2512:2631	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	0	120	theme	bifidum	17:23	arg1	TMC3115					25:31	[Bifidobacterium bifidum TMC3115	0:31	[Bifidobacterium bifidum TMC3115	0:31	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	12	121	dep	higher	2234:2239	arg1	P<0.05					2243:2248	P<0.05	2243:2248	P<0.05	2243:2248	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	11	122	theme	inflammatory	1882:1893	arg1	infiltration					1895:1906	less inflammatory infiltration	1877:1906	less inflammatory infiltration	1877:1906	Results 1) At the end of the experiment, the inflammatory pathology score was significantly lower in the TMC3115-DSS group compared with that of the Saline-DSS group ( P<0.05), with less disruption of colonic crypt structures and other structures, less inflammatory infiltration, and more intact epithelial structures.
36224686	12	123	theme	relative	2114:2121	arg1	abundance					2123:2131	the relative abundance	2110:2131	the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05)	2110:2189	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	2	124	theme	BALB/c	400:405	arg1	mice					407:410	84 newborn BALB/c mice	389:410	84 newborn BALB/c mice	389:410	Methods Fourteen pregnant BALB/c mice were purchased and 84 newborn BALB/c mice were subsequently obtained.
36224686	12	125	from	abundance	2018:2026	arg1	feces					2078:2082	the feces	2074:2082	the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10	2074:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	13	126	theme	experiment	2399:2408	arg1	end					2388:2390	the end	2384:2390	the end of the experiment	2384:2408	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	14	127	from	use	2781:2783	arg1	life					2805:2808	early life	2799:2808	early life	2799:2808	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	12	128	theme	Staphylococcuswas	2157:2173	arg1	IL					2269:2270	IL	2269:2270	IL	2269:2270	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	128	theme	Staphylococcuswas	2157:2173	arg1	abundance					2123:2131	the relative abundance	2110:2131	the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05)	2110:2189	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	128	theme	Staphylococcuswas	2157:2173	arg1	P<0.05					2101:2106	P<0.05	2101:2106	P<0.05	2101:2106	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	128	theme	Staphylococcuswas	2157:2173	arg1	interleukin					2256:2266	interleukin (IL)-10	2256:2274	interleukin (IL)-10	2256:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	128	theme	Staphylococcuswas	2157:2173	arg1	TMC3115					2091:2097	the TMC3115	2087:2097	the TMC3115 ( P<0.05)	2087:2107	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	2	129	theme	newborn	392:398	arg1	mice					407:410	84 newborn BALB/c mice	389:410	84 newborn BALB/c mice	389:410	Methods Fourteen pregnant BALB/c mice were purchased and 84 newborn BALB/c mice were subsequently obtained.
36224686	1	130	theme	bowel	317:321	arg1	disease					323:329	inflammatory bowel disease	304:329	inflammatory bowel disease	304:329	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	13	131	theme	tumefaciens	2573:2583	arg1	abundance					2529:2537	reduced relative abundance	2512:2537	reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05)	2512:2631	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	3	132	theme	daily	605:609	arg1	volume					611:616	a daily volume	603:616	a daily volume of 0.2 mL for each mouse	603:641	Then, the newborn mice were randomly assigned to a normal saline (NS) group and a TMC3115 group, given via oral gavage normal saline and TMC3115, respectively, at a daily volume of 0.2 mL for each mouse.
36224686	7	133	theme	3	1043:1043	arg1	%					1044:1044	%	1044:1044	%	1044:1044	The mice were given regular feed until the end of week 6 when they were given 3% dextran sulphate sodium (DSS) ad libitum for 4 days to establish the enteritis model, while the non-modeling groups were given pure water ad libitum.
36224686	0	134	theme	Microbiota	64:73	arg1	Building					75:82	Microbiota Building	64:82	Microbiota Building	64:82	[Bifidobacterium bifidum TMC3115 Promotes Early Life Intestinal Microbiota Building to Alleviate Symptoms of Inflammatory Bowel Disease].
36224686	1	135	dep	Bifidobacterium	184:198	arg1	bifidum					200:206	bifidum	200:206	bifidum	200:206	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36224686	10	136	from	weeks	1409:1413	arg1	spleen					1375:1380	spleen	1375:1380	spleen	1375:1380	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	136	from	weeks	1409:1413	arg1	samples					1366:1372	the fecal samples	1356:1372	the fecal samples	1356:1372	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	136	from	weeks	1409:1413	arg1	tissue					1316:1321	The intestinal tissue	1301:1321	The intestinal tissue at the end of the experiment	1301:1350	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	136	from	weeks	1409:1413	arg1	serum					1386:1390	serum	1386:1390	serum	1386:1390	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	137	theme	cytokines	1618:1626	arg1	concentration					1595:1607	the mass concentration	1586:1607	the mass concentration of serum cytokines	1586:1626	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	137	theme	cytokines	1618:1626	arg1	index					1576:1580	spleen organ index	1563:1580	spleen organ index	1563:1580	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	137	theme	cytokines	1618:1626	arg1	inflammation					1508:1519	colonic inflammation	1500:1519	colonic inflammation	1500:1519	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	10	137	theme	cytokines	1618:1626	arg1	composition					1526:1536	the composition	1522:1536	the composition of fecal gut microbiota	1522:1560	The intestinal tissue at the end of the experiment and the fecal samples, spleen and serum of the mice at 3 weeks and at the end of the experiment were collected to determine the pathology scores of colonic inflammation, the composition of fecal gut microbiota, spleen organ index and the mass concentration of serum cytokines.
36224686	2	138	theme	pregnant	349:356	arg1	mice					365:368	Methods Fourteen pregnant BALB/c mice	332:368	Methods Fourteen pregnant BALB/c mice	332:368	Methods Fourteen pregnant BALB/c mice were purchased and 84 newborn BALB/c mice were subsequently obtained.
36224686	14	139	from	construction	2823:2834	arg1	mice					2866:2869	neonatal mice	2857:2869	neonatal mice	2857:2869	Conclusion The use of TMC3115 in early life promotes the construction of gut microbiota in neonatal mice, thereby producing a long-term effect that alleviates colitis in mice, but the mechanisms involved are still not fully understood.
36224686	12	140	theme	higher	2234:2239	arg1	feces					2078:2082	the feces	2074:2082	the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10	2074:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	12	141	theme	TMC3115	2091:2097	arg1	feces					2078:2082	the feces	2074:2082	the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10	2074:2274	2) At 3 weeks, in comparison with those of the NS group, the relative abundance of Bifidobacteriumwas significantly higher in the feces of the TMC3115 ( P<0.05), the relative abundance of both Enterococcusand Staphylococcuswas lower ( P<0.05), the splenic organ index was significantly higher ( P<0.05), and interleukin (IL)-10 was significantly decreased ( P<0.05), while there was no significant change in IL-6 or TNF-α ( P>0.05).
36224686	6	142	from	mice	811:814	arg1	group					824:828	each group	819:828	each group	819:828	At this point, half of the mice in each group were sacrificed, and then the remaining mice in each group were randomly divided into NS-water group, NS-DSS group, TMC3115-water group, and TMC3115-DSS group, with about 10 mice in each group.
36224686	13	143	dep	higher	2682:2687	arg1	P<0.05					2691:2696	P<0.05	2691:2696	P<0.05	2691:2696	At the end of the experiment, in comparison with those of the NS-DSS group that undergone DSS induction, the TMC3115-DSS group had reduced relative abundance of Staphylococcus, Staphylococcus tumefaciens and Escherichia/ Shigellain the feces ( P<0.05), while the splenic organ index was significantly higher ( P<0.05), and there were no significant changes in IL-6 or TNF-α ( P>0.05).
36224686	1	144	theme	immune	259:264	arg1	functions					266:274	immune functions	259:274	immune functions	259:274	Objective To investigate the effects of using Bifidobacterium bifidum TMC3115 in early life on intestinal microbiota and immune functions and the long-term impact on inflammatory bowel disease.
36331062	10	0	theme	Increasing	1544:1553	arg1	levels					1555:1560	Increasing levels	1544:1560	Increasing levels of microbial phytase	1544:1581	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	2	1	theme	phytate-bound	497:509	arg1	P					511:511	phytate-bound P	497:511	0.23% phytate-bound P	491:511	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	4	2	with	design	841:846	arg1	periods					880:886	three 11-day periods	867:886	three 11-day periods	867:886	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	4	2	with	design	841:846	arg1	diets					857:861	six diets	853:861	six diets	853:861	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	14	3	theme	phytase	2197:2203	arg1	concentration					2172:2184	concentration	2172:2184	concentration of dietary phytase	2172:2203	The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased.
36331062	1	4	theme	ileal	254:258	arg1	digestibility					260:272	the apparent ileal digestibility	241:272	the apparent ileal digestibility (AID)	241:278	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	2	5	dep	phytate	476:482	arg1	%					474:474	%	474:474	%	474:474	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	10	6	theme	phytase	1575:1581	arg1	levels					1555:1560	Increasing levels	1544:1560	Increasing levels of microbial phytase	1544:1581	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	13	7	theme	P	1955:1955	arg1	<					1957:1957	P < 0.05	1955:1962	P < 0.05	1955:1962	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	8	8	theme	microbial	1388:1396	arg1	phytase					1398:1404	microbial phytase	1388:1404	microbial phytase	1388:1404	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	10	9	dep	Ca	1657:1658	arg1	i.e.					1651:1654	i.e.	1651:1654	i.e.	1651:1654	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	2	10	from	acids	595:599	arg1	deficient					535:543	deficient	535:543	deficient	535:543	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	10	from	acids	595:599	arg1	diet					391:394	A negative control (NC) diet	367:394	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P)	367:512	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	13	11	from	ATTD	1981:1984	arg1	diets					2027:2031	experimental diets	2014:2031	experimental diets	2014:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	15	12	theme	energy	2393:2398	arg1	ATTD					2379:2382	the ATTD	2375:2382	the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA	2375:2464	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	4	13	theme	ileal-cannulated	752:767	arg1	1.71 kg					783:789	17.81 ± 1.71 kg	775:789	17.81 ± 1.71 kg	775:789	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	4	13	theme	ileal-cannulated	752:767	arg1	pigs					769:772	Eighteen ileal-cannulated pigs	743:772	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg)	743:790	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	0	14	theme	consensus	99:107	arg1	variant					129:135	a novel consensus bacterial 6-phytase variant	91:135	a novel consensus bacterial 6-phytase variant	91:135	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	10	15	dep	minerals	1641:1648	arg1	P					1661:1661	P	1661:1661	P	1661:1661	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	10	15	dep	minerals	1641:1648	arg1	Ca					1657:1658	Ca	1657:1658	Ca	1657:1658	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	10	15	dep	minerals	1641:1648	arg1	Mg					1667:1668	Mg	1667:1668	Mg	1667:1668	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	10	15	dep	minerals	1641:1648	arg1	Cu					1671:1672	Cu	1671:1672	Cu	1671:1672	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	10	15	dep	minerals	1641:1648	arg1	K					1664:1664	K	1664:1664	K	1664:1664	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	14	16	theme	P	2159:2159	arg1	<					2161:2161	P < 0.05	2159:2166	P < 0.05	2159:2166	The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased.
36331062	11	17	theme	phytase	1788:1794	arg1	level					1779:1783	inclusion level	1769:1783	inclusion level of phytase	1769:1794	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	0	18	theme	6-phytase	119:127	arg1	variant					129:135	a novel consensus bacterial 6-phytase variant	91:135	a novel consensus bacterial 6-phytase variant	91:135	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	1	19	theme	young	355:359	arg1	pigs					361:364	young pigs	355:364	young pigs	355:364	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	13	20	theme	P	1998:1998	arg1	ATTD					1981:1984	the ATTD	1977:1984	the ATTD of ash, Ca, P, K, and Cu in experimental diets	1977:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	12	21	theme	microbial	1841:1849	arg1	phytase					1851:1857	microbial phytase	1841:1857	microbial phytase	1841:1857	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	7	22	theme	anal	1119:1122	arg1	stimulation					1124:1134	anal stimulation	1119:1134	anal stimulation on days 6, 7, 8, and 9	1119:1157	For each period, fecal samples were collected via anal stimulation on days 6, 7, 8, and 9, whereas ileal digesta were collected on days 10 and 11 using standard procedures.
36331062	2	23	theme	amino	589:593	arg1	AA					602:603	AA	602:603	AA	602:603	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	23	theme	amino	589:593	arg1	acids					595:599	standardized ileal digestible amino acids	559:599	standardized ileal digestible amino acids (AA)	559:604	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	13	24	from	increase	1965:1972	arg1	ATTD					1981:1984	the ATTD	1977:1984	the ATTD of ash, Ca, P, K, and Cu in experimental diets	1977:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	2	25	theme	ileal	572:576	arg1	AA					602:603	AA	602:603	AA	602:603	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	25	theme	ileal	572:576	arg1	acids					595:599	standardized ileal digestible amino acids	559:599	standardized ileal digestible amino acids (AA)	559:604	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	15	26	theme	novel	2235:2239	arg1	phytase					2251:2257	the novel microbial phytase	2231:2257	the novel microbial phytase used in this experiment	2231:2281	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	26	theme	novel	2235:2239	arg1	effective					2287:2295	effective	2287:2295	effective	2287:2295	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	14	27	theme	GE	2132:2133	arg1	ATTD					2117:2120	The ATTD	2113:2120	The ATTD of Mg and GE	2113:2133	The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased.
36331062	10	28	theme	dry	1626:1628	arg1	matter					1630:1635	dry matter	1626:1635	dry matter	1626:1635	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	0	29	theme	nutrients	50:58	arg1	digestibility					22:34	Ileal and total tract digestibility	0:34	Ileal and total tract digestibility of energy and nutrients in pig	0:65	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	8	30	dep	increased	1332:1340	arg1	quadratic					1343:1351	quadratic	1343:1351	quadratic	1343:1351	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	3	31	theme	phytase	718:724	arg1	units/kg					696:703	250, 500, 1,000, 2,000, or 4,000 phytase units/kg	655:703	250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase	655:724	Five additional diets were formulated by adding 250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase to the NC diets.
36331062	9	32	theme	Dietary	1430:1436	arg1	inclusion					1438:1446	Dietary inclusion	1430:1446	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg	1430:1492	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	5	33	dep	pigs	973:976	arg1	replicate					963:971	replicate	963:971	replicate	963:971	There were three pigs per diet in each period; therefore, there were nine replicate pigs per diet.
36331062	13	34	from	P	1998:1998	arg1	diets					2027:2031	experimental diets	2014:2031	experimental diets	2014:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	4	35	theme	±	781:781	arg1	1.71 kg					783:789	17.81 ± 1.71 kg	775:789	17.81 ± 1.71 kg	775:789	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	4	35	theme	±	781:781	arg1	pigs					769:772	Eighteen ileal-cannulated pigs	743:772	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg)	743:790	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	10	36	dep	the	1615:1617	arg1	AID					1619:1621	AID	1619:1621	AID	1619:1621	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	15	37	from	conclusion	2219:2228	arg1	phytase					2251:2257	the novel microbial phytase	2231:2257	the novel microbial phytase used in this experiment	2231:2281	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	37	from	conclusion	2219:2228	arg1	effective					2287:2295	effective	2287:2295	effective	2287:2295	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	38	theme	microbial	2241:2249	arg1	phytase					2251:2257	the novel microbial phytase	2231:2257	the novel microbial phytase used in this experiment	2231:2281	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	38	theme	microbial	2241:2249	arg1	effective					2287:2295	effective	2287:2295	effective	2287:2295	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	39	theme	minerals	2404:2411	arg1	ATTD					2379:2382	the ATTD	2375:2382	the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA	2375:2464	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	9	40	theme	novel	1455:1459	arg1	phytase					1461:1467	the novel phytase	1451:1467	the novel phytase at 1,000 or 2,000 FTU/kg	1451:1492	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	10	41	dep	quadratic	1594:1602	arg1	<					1607:1607	P < 0.05	1605:1612	P < 0.05	1605:1612	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	13	42	theme	Cu	2008:2009	arg1	ATTD					1981:1984	the ATTD	1977:1984	the ATTD of ash, Ca, P, K, and Cu in experimental diets	1977:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	9	43	dep	79.8	1537:1540	arg1	to					1534:1535	to	1534:1535	to	1534:1535	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	7	44	theme	fecal	1086:1090	arg1	samples					1092:1098	fecal samples	1086:1098	fecal samples	1086:1098	For each period, fecal samples were collected via anal stimulation on days 6, 7, 8, and 9, whereas ileal digesta were collected on days 10 and 11 using standard procedures.
36331062	4	45	theme	square	834:839	arg1	design					841:846	a 6 × 3 incomplete Latin square design	809:846	a 6 × 3 incomplete Latin square design with six diets and three 11-day periods	809:886	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	0	46	from	digestibility	22:34	arg1	pig					63:65	pig	63:65	pig	63:65	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	0	47	theme	Ileal	0:4	arg1	digestibility					22:34	Ileal and total tract digestibility	0:34	Ileal and total tract digestibility of energy and nutrients in pig	0:65	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	2	48	theme	NC	387:388	arg1	deficient					535:543	deficient	535:543	deficient	535:543	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	48	theme	NC	387:388	arg1	diet					391:394	A negative control (NC) diet	367:394	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P)	367:512	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	49	theme	control	378:384	arg1	deficient					535:543	deficient	535:543	deficient	535:543	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	49	theme	control	378:384	arg1	diet					391:394	A negative control (NC) diet	367:394	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P)	367:512	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	0	50	theme	total	10:14	arg1	digestibility					22:34	Ileal and total tract digestibility	0:34	Ileal and total tract digestibility of energy and nutrients in pig	0:65	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	15	51	from	effective	2287:2295	arg1	conclusion					2219:2228	conclusion	2219:2228	conclusion	2219:2228	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	4	52	theme	×	813:813	arg1	design					841:846	a 6 × 3 incomplete Latin square design	809:846	a 6 × 3 incomplete Latin square design with six diets and three 11-day periods	809:886	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	10	53	dep	increased	1583:1591	arg1	quadratic					1594:1602	quadratic	1594:1602	quadratic	1594:1602	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	3	54	theme	phytase	688:694	arg1	units/kg					696:703	250, 500, 1,000, 2,000, or 4,000 phytase units/kg	655:703	250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase	655:724	Five additional diets were formulated by adding 250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase to the NC diets.
36331062	15	55	from	AA	2463:2464	arg1	deficient					2439:2447	deficient	2439:2447	deficient	2439:2447	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	7	56	theme	standard	1221:1228	arg1	procedures					1230:1239	standard procedures	1221:1239	standard procedures	1221:1239	For each period, fecal samples were collected via anal stimulation on days 6, 7, 8, and 9, whereas ileal digesta were collected on days 10 and 11 using standard procedures.
36331062	4	57	theme	incomplete	817:826	arg1	design					841:846	a 6 × 3 incomplete Latin square design	809:846	a 6 × 3 incomplete Latin square design with six diets and three 11-day periods	809:886	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	13	58	theme	experimental	2014:2025	arg1	diets					2027:2031	experimental diets	2014:2031	experimental diets	2014:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	14	59	dep	increased	2140:2148	arg1	linear					2151:2156	linear	2151:2156	linear	2151:2156	The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased.
36331062	11	60	from	increase	1708:1715	arg1	the					1731:1733	the	1731:1733	the	1731:1733	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	0	61	theme	energy	39:44	arg1	digestibility					22:34	Ileal and total tract digestibility	0:34	Ileal and total tract digestibility of energy and nutrients in pig	0:65	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	1	62	theme	total	293:297	arg1	ATTD					320:323	ATTD	320:323	ATTD	320:323	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	1	62	theme	total	293:297	arg1	digestibility					305:317	apparent total tract digestibility	284:317	apparent total tract digestibility (ATTD)	284:324	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	13	63	from	diets	2027:2031	arg1	ATTD					1981:1984	the ATTD	1977:1984	the ATTD of ash, Ca, P, K, and Cu in experimental diets	1977:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	13	64	from	K	2001:2001	arg1	diets					2027:2031	experimental diets	2014:2031	experimental diets	2014:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	14	65	dep	linear	2151:2156	arg1	<					2161:2161	P < 0.05	2159:2166	P < 0.05	2159:2166	The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased.
36331062	15	66	from	deficient	2439:2447	arg1	P					2456:2456	P	2456:2456	P	2456:2456	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	66	from	deficient	2439:2447	arg1	AA					2463:2464	AA	2463:2464	AA	2463:2464	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	66	from	deficient	2439:2447	arg1	Ca					2452:2453	Ca	2452:2453	Ca	2452:2453	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	15	67	dep	the	2311:2313	arg1	AID					2315:2317	AID	2315:2317	AID	2315:2317	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	8	68	theme	indispensable	1291:1303	arg1	AA					1305:1306	indispensable AA	1291:1306	indispensable AA	1291:1306	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	1	69	theme	novel	217:221	arg1	phytase					223:229	a novel phytase	215:229	a novel phytase	215:229	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	11	70	theme	P	1718:1718	arg1	increase					1708:1715	a linear increase	1699:1715	a linear increase (P < 0.05) in the AID of ash and Na	1699:1751	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	11	70	theme	P	1718:1718	arg1	<					1720:1720	P < 0.05	1718:1725	P < 0.05	1718:1725	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	13	71	from	ash	1989:1991	arg1	diets					2027:2031	experimental diets	2014:2031	experimental diets	2014:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	2	72	contain	contained	446:454	arg1	meal					436:439	canola meal	429:439	canola meal	429:439	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	72	contain	contained	446:454	arg2	phytate					476:482	approximately 0.83% phytate	456:482	approximately 0.83% phytate	456:482	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	72	contain	contained	446:454	arg1	meal					419:422	meal	419:422	meal	419:422	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	72	contain	contained	446:454	arg1	corn					405:408	corn	405:408	corn	405:408	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	15	73	from	P	2456:2456	arg1	deficient					2439:2447	deficient	2439:2447	deficient	2439:2447	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	9	74	from	FTU/kg	1487:1492	arg1	inclusion					1438:1446	Dietary inclusion	1430:1446	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg	1430:1492	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	9	74	from	FTU/kg	1487:1492	arg1	phytase					1461:1467	the novel phytase	1451:1467	the novel phytase at 1,000 or 2,000 FTU/kg	1451:1492	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	8	75	theme	dispensable	1313:1323	arg1	AA					1325:1326	dispensable AA	1313:1326	dispensable AA	1313:1326	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	6	76	theme	adaptation	1038:1047	arg1	period					1049:1054	an adaptation period	1035:1054	an adaptation period to the diet	1035:1066	The initial 5 d of each period were considered an adaptation period to the diet.
36331062	1	77	theme	apparent	245:252	arg1	digestibility					260:272	the apparent ileal digestibility	241:272	the apparent ileal digestibility (AID)	241:278	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	7	78	theme	ileal	1168:1172	arg1	digesta					1174:1180	ileal digesta	1168:1180	ileal digesta	1168:1180	For each period, fecal samples were collected via anal stimulation on days 6, 7, 8, and 9, whereas ileal digesta were collected on days 10 and 11 using standard procedures.
36331062	14	79	theme	dietary	2189:2195	arg1	phytase					2197:2203	dietary phytase	2189:2203	dietary phytase	2189:2203	The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased.
36331062	11	80	theme	inclusion	1769:1777	arg1	level					1779:1783	inclusion level	1769:1783	inclusion level of phytase	1769:1794	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	13	81	theme	microbial	2071:2079	arg1	phytase					2081:2087	microbial phytase	2071:2087	microbial phytase	2071:2087	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	2	82	dep	%	474:474	arg1	0.83					470:473	0.83	470:473	0.83	470:473	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	13	83	from	Ca	1994:1995	arg1	diets					2027:2031	experimental diets	2014:2031	experimental diets	2014:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	10	84	theme	microbial	1565:1573	arg1	phytase					1575:1581	microbial phytase	1565:1581	microbial phytase	1565:1581	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	6	85	theme	period	1012:1017	arg1	d					1002:1002	The initial 5 d	988:1002	The initial 5 d of each period	988:1017	The initial 5 d of each period were considered an adaptation period to the diet.
36331062	15	86	theme	gross	2387:2391	arg1	energy					2393:2398	gross energy	2387:2398	gross energy	2387:2398	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	10	87	theme	P	1605:1605	arg1	<					1607:1607	P < 0.05	1605:1612	P < 0.05	1605:1612	Increasing levels of microbial phytase increased (quadratic, P < 0.05) the AID of dry matter and minerals (i.e., Ca, P, K, Mg, Cu) in the diets.
36331062	0	88	theme	bacterial	109:117	arg1	variant					129:135	a novel consensus bacterial 6-phytase variant	91:135	a novel consensus bacterial 6-phytase variant	91:135	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	8	89	theme	phytase	1398:1404	arg1	concentration					1371:1383	the concentration	1367:1383	the concentration of microbial phytase	1367:1404	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	3	90	theme	additional	612:621	arg1	diets					623:627	Five additional diets	607:627	Five additional diets	607:627	Five additional diets were formulated by adding 250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase to the NC diets.
36331062	12	91	dep	linear	1870:1875	arg1	<					1880:1880	P < 0.01	1878:1885	P < 0.01	1878:1885	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	7	92	dep	collected	1105:1113	arg1	whereas					1160:1166	whereas	1160:1166	whereas	1160:1166	For each period, fecal samples were collected via anal stimulation on days 6, 7, 8, and 9, whereas ileal digesta were collected on days 10 and 11 using standard procedures.
36331062	2	93	dep	%	495:495	arg1	P					511:511	phytate-bound P	497:511	0.23% phytate-bound P	491:511	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	93	dep	%	495:495	arg1	i.e.					485:488	i.e.	485:488	i.e.	485:488	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	12	94	theme	phytase	1851:1857	arg1	levels					1831:1836	Increasing levels	1820:1836	Increasing levels of microbial phytase	1820:1857	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	12	95	dep	increased	1859:1867	arg1	linear					1870:1875	linear	1870:1875	linear	1870:1875	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	6	96	theme	initial	992:998	arg1	d					1002:1002	The initial 5 d	988:1002	The initial 5 d of each period	988:1017	The initial 5 d of each period were considered an adaptation period to the diet.
36331062	2	97	theme	digestible	578:587	arg1	AA					602:603	AA	602:603	AA	602:603	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	97	theme	digestible	578:587	arg1	acids					595:599	standardized ileal digestible amino acids	559:599	standardized ileal digestible amino acids (AA)	559:604	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	98	from	deficient	535:543	arg1	Ca					548:549	Ca	548:549	Ca	548:549	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	98	from	deficient	535:543	arg1	AA					602:603	AA	602:603	AA	602:603	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	98	from	deficient	535:543	arg1	acids					595:599	standardized ileal digestible amino acids	559:599	standardized ileal digestible amino acids (AA)	559:604	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	98	from	deficient	535:543	arg1	P					552:552	P	552:552	P	552:552	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	8	99	dep	the	1265:1267	arg1	AID					1269:1271	AID	1269:1271	AID	1269:1271	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	12	100	theme	Increasing	1820:1829	arg1	levels					1831:1836	Increasing levels	1820:1836	Increasing levels of microbial phytase	1820:1857	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	2	101	from	Ca	548:549	arg1	deficient					535:543	deficient	535:543	deficient	535:543	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	101	from	Ca	548:549	arg1	diet					391:394	A negative control (NC) diet	367:394	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P)	367:512	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	9	102	theme	total	1515:1519	arg1	AA					1521:1522	total AA	1515:1522	total AA	1515:1522	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	13	103	dep	quadratic	1944:1952	arg1	<					1957:1957	P < 0.05	1955:1962	P < 0.05	1955:1962	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	8	104	dep	quadratic	1343:1351	arg1	<					1356:1356	P < 0.05	1354:1361	P < 0.05	1354:1361	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	2	105	from	P	552:552	arg1	deficient					535:543	deficient	535:543	deficient	535:543	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	105	from	P	552:552	arg1	diet					391:394	A negative control (NC) diet	367:394	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P)	367:512	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	13	106	theme	phytase	2081:2087	arg1	concentration					2054:2066	the concentration	2050:2066	the concentration of microbial phytase	2050:2087	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	1	107	dep	digestibility	260:272	arg1	AID					275:277	AID	275:277	AID	275:277	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	13	108	theme	K	2001:2001	arg1	ATTD					1981:1984	the ATTD	1977:1984	the ATTD of ash, Ca, P, K, and Cu in experimental diets	1977:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	12	109	dep	the	1888:1890	arg1	AID					1892:1894	AID	1892:1894	AID	1892:1894	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	3	110	theme	novel	712:716	arg1	phytase					718:724	the novel phytase	708:724	the novel phytase	708:724	Five additional diets were formulated by adding 250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase to the NC diets.
36331062	0	111	theme	novel	93:97	arg1	variant					129:135	a novel consensus bacterial 6-phytase variant	91:135	a novel consensus bacterial 6-phytase variant	91:135	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	1	112	theme	nutrients	329:337	arg1	ATTD					320:323	ATTD	320:323	ATTD	320:323	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	1	112	theme	nutrients	329:337	arg1	digestibility					260:272	the apparent ileal digestibility	241:272	the apparent ileal digestibility (AID)	241:278	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	1	112	theme	nutrients	329:337	arg1	digestibility					305:317	apparent total tract digestibility	284:317	apparent total tract digestibility (ATTD)	284:324	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	15	113	from	ATTD	2379:2382	arg1	diets					2416:2420	diets	2416:2420	diets formulated to be deficient in Ca, P, and AA	2416:2464	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	14	114	theme	Mg	2125:2126	arg1	ATTD					2117:2120	The ATTD	2113:2120	The ATTD of Mg and GE	2113:2133	The ATTD of Mg and GE also increased (linear; P < 0.05) as concentration of dietary phytase increased.
36331062	2	115	theme	canola	429:434	arg1	meal					436:439	canola meal	429:439	canola meal	429:439	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	116	theme	standardized	559:570	arg1	AA					602:603	AA	602:603	AA	602:603	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	116	theme	standardized	559:570	arg1	acids					595:599	standardized ileal digestible amino acids	559:599	standardized ileal digestible amino acids (AA)	559:604	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	12	117	theme	P	1878:1878	arg1	<					1880:1880	P < 0.01	1878:1885	P < 0.01	1878:1885	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	13	118	theme	Ca	1994:1995	arg1	ATTD					1981:1984	the ATTD	1977:1984	the ATTD of ash, Ca, P, K, and Cu in experimental diets	1977:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	0	119	dep	diets	67:71	arg1	supplemented					73:84	supplemented	73:84	diets supplemented with a novel consensus bacterial 6-phytase variant	67:135	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	3	120	theme	NC	733:734	arg1	diets					736:740	the NC diets	729:740	the NC diets	729:740	Five additional diets were formulated by adding 250, 500, 1,000, 2,000, or 4,000 phytase units/kg of the novel phytase to the NC diets.
36331062	15	121	theme	dry	2322:2324	arg1	matter					2326:2331	dry matter	2322:2331	dry matter	2322:2331	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	4	122	theme	Latin	828:832	arg1	design					841:846	a 6 × 3 incomplete Latin square design	809:846	a 6 × 3 incomplete Latin square design with six diets and three 11-day periods	809:886	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	0	123	theme	tract	16:20	arg1	digestibility					22:34	Ileal and total tract digestibility	0:34	Ileal and total tract digestibility of energy and nutrients in pig	0:65	Ileal and total tract digestibility of energy and nutrients in pig diets supplemented with a novel consensus bacterial 6-phytase variant.
36331062	7	124	from	stimulation	1124:1134	arg1	days					1139:1142	days 6, 7, 8, and 9	1139:1157	days 6, 7, 8, and 9	1139:1157	For each period, fecal samples were collected via anal stimulation on days 6, 7, 8, and 9, whereas ileal digesta were collected on days 10 and 11 using standard procedures.
36331062	11	125	theme	linear	1701:1706	arg1	increase					1708:1715	a linear increase	1699:1715	a linear increase (P < 0.05) in the AID of ash and Na	1699:1751	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	11	125	theme	linear	1701:1706	arg1	<					1720:1720	P < 0.05	1718:1725	P < 0.05	1718:1725	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	11	126	dep	the	1731:1733	arg1	AID					1735:1737	AID	1735:1737	AID	1735:1737	Likewise, a linear increase (P < 0.05) in the AID of ash and Na was observed as inclusion level of phytase increased in the diets.
36331062	2	127	theme	negative	369:376	arg1	deficient					535:543	deficient	535:543	deficient	535:543	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	127	theme	negative	369:376	arg1	diet					391:394	A negative control (NC) diet	367:394	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P)	367:512	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	2	128	dep	contained	446:454	arg1	%					495:495	0.23%	491:495	0.23% phytate-bound P	491:511	A negative control (NC) diet based on corn, soybean meal, and canola meal that contained approximately 0.83% phytate (i.e., 0.23% phytate-bound P) was formulated to be deficient in Ca, P, and standardized ileal digestible amino acids (AA).
36331062	13	129	from	Cu	2008:2009	arg1	diets					2027:2031	experimental diets	2014:2031	experimental diets	2014:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	8	130	theme	crude	1276:1280	arg1	protein					1282:1288	crude protein	1276:1288	crude protein	1276:1288	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	9	131	theme	phytase	1461:1467	arg1	inclusion					1438:1446	Dietary inclusion	1430:1446	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg	1430:1492	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	13	132	theme	ash	1989:1991	arg1	ATTD					1981:1984	the ATTD	1977:1984	the ATTD of ash, Ca, P, K, and Cu in experimental diets	1977:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	1	133	theme	apparent	284:291	arg1	ATTD					320:323	ATTD	320:323	ATTD	320:323	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	1	133	theme	apparent	284:291	arg1	digestibility					305:317	apparent total tract digestibility	284:317	apparent total tract digestibility (ATTD)	284:324	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	15	134	from	Ca	2452:2453	arg1	deficient					2439:2447	deficient	2439:2447	deficient	2439:2447	In conclusion, the novel microbial phytase used in this experiment was effective in increasing the AID of dry matter, GE, starch, minerals, and AA, as well as the ATTD of gross energy and minerals in diets formulated to be deficient in Ca, P, and AA.
36331062	13	135	theme	quadratic	1944:1952	arg1	increase					1965:1972	A quadratic (P < 0.05) increase	1942:1972	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets	1942:2031	A quadratic (P < 0.05) increase in the ATTD of ash, Ca, P, K, and Cu in experimental diets were observed as the concentration of microbial phytase increased in the diets.
36331062	12	136	theme	gross	1899:1903	arg1	GE					1913:1914	GE	1913:1914	GE	1913:1914	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	12	136	theme	gross	1899:1903	arg1	energy					1905:1910	gross energy	1899:1910	gross energy (GE)	1899:1915	Increasing levels of microbial phytase increased (linear, P < 0.01) the AID of gross energy (GE) and starch in the diets.
36331062	1	137	theme	tract	299:303	arg1	ATTD					320:323	ATTD	320:323	ATTD	320:323	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	1	137	theme	tract	299:303	arg1	digestibility					305:317	apparent total tract digestibility	284:317	apparent total tract digestibility (ATTD)	284:324	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
36331062	9	138	from	inclusion	1438:1446	arg1	FTU/kg					1487:1492	1,000 or 2,000 FTU/kg	1472:1492	1,000 or 2,000 FTU/kg	1472:1492	Dietary inclusion of the novel phytase at 1,000 or 2,000 FTU/kg increased the AID of total AA from 73.7 to 79.8%.
36331062	4	139	theme	11-day	873:878	arg1	periods					880:886	three 11-day periods	867:886	three 11-day periods	867:886	Eighteen ileal-cannulated pigs (17.81 ± 1.71 kg) were allotted to a 6 × 3 incomplete Latin square design with six diets and three 11-day periods.
36331062	8	140	theme	P	1354:1354	arg1	<					1356:1356	P < 0.05	1354:1361	P < 0.05	1354:1361	Results indicated that the AID of crude protein, indispensable AA, and dispensable AA was increased (quadratic, P < 0.05) as the concentration of microbial phytase increased in the diets.
36331062	1	141	theme	phytase	223:229	arg1	levels					205:210	levels	205:210	levels of a novel phytase	205:229	An experiment was conducted to test the hypothesis that increasing levels of a novel phytase increases the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of nutrients in diets fed to young pigs.
37331158	9	0	theme	lymph	1605:1609	arg1	node					1611:1614	lymph node	1605:1614	lymph node imaging	1605:1622	This study has important implications for lymph node imaging and surgical navigation.
37331158	6	1	theme	sentinel	1072:1079	arg1	node					1087:1090	metastatic sentinel lymph node	1061:1090	metastatic sentinel lymph node from normal	1061:1102	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	2	2	theme	short	364:368	arg1	time					380:383	short retention time	364:383	short retention time	364:383	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	6	3	theme	ICG	881:883	arg1	nanoprobes					885:894	The FA-HA/ZIF-8@ICG nanoprobes	865:894	The FA-HA/ZIF-8@ICG nanoprobes	865:894	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	9	4	theme	surgical	1628:1635	arg1	navigation					1637:1646	surgical navigation	1628:1646	surgical navigation	1628:1646	This study has important implications for lymph node imaging and surgical navigation.
37331158	5	5	theme	tumor	803:807	arg1	cells					809:813	the tumor cells	799:813	the tumor cells	799:813	The coupled hyaluronic acid and folic acid can target to the tumor cells and dentritic cells with a dual-targeting effect.
37331158	7	6	dep	time	1351:1354	arg1	imaging					1364:1370	in vivo imaging	1356:1370	long retention time in vivo imaging	1336:1370	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	6	7	theme	FA-HA/ZIF-8	869:879	arg1	nanoprobes					885:894	The FA-HA/ZIF-8@ICG nanoprobes	865:894	The FA-HA/ZIF-8@ICG nanoprobes	865:894	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	7	8	from	ICG	1254:1256	arg1	energy					1242:1247	the absorbed excitation energy	1218:1247	the absorbed excitation energy from ICG	1218:1256	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	4	9	theme	hyaluronic	677:686	arg1	acid					688:691	hyaluronic acid	677:691	hyaluronic acid	677:691	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	6	10	theme	luminescence	958:969	arg1	times					986:990	1.6 times	982:990	1.6 times	982:990	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	6	10	theme	luminescence	958:969	arg1	intensity					971:979	a stronger luminescence intensity	947:979	a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes	947:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	6	11	theme	metastatic	1061:1070	arg1	node					1087:1090	metastatic sentinel lymph node	1061:1090	metastatic sentinel lymph node from normal	1061:1102	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	8	12	theme	@	1398:1398	arg1	nanoplatform					1405:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	7	13	theme	in	1356:1357	arg1	imaging					1364:1370	in vivo imaging	1356:1370	long retention time in vivo imaging	1336:1370	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	1	14	theme	postoperative	233:245	arg1	metastasis					247:256	postoperative metastasis	233:256	postoperative metastasis	233:256	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	8	15	dep	depth	1444:1448	arg1	the					1428:1430	the	1428:1430	the	1428:1430	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	7	16	theme	retention	1341:1349	arg1	time					1351:1354	long retention time	1336:1354	long retention time in vivo imaging	1336:1370	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	9	17	contain	has	1574:1576	arg2	implications					1588:1599	important implications	1578:1599	important implications for lymph node imaging and surgical navigation	1578:1646	This study has important implications for lymph node imaging and surgical navigation.
37331158	9	17	contain	has	1574:1576	arg1	study					1568:1572	This study	1563:1572	This study	1563:1572	This study has important implications for lymph node imaging and surgical navigation.
37331158	6	18	theme	sentinel	922:929	arg1	node					937:940	sentinel lymph node	922:940	sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes	922:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	4	19	theme	lymph	729:733	arg1	nodes					735:739	metastatic lymph nodes	718:739	metastatic lymph nodes	718:739	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	2	20	theme	lymph	286:290	arg1	node					292:295	clinical sentinel lymph node	268:295	clinical sentinel lymph node imaging	268:303	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	1	21	theme	lymph	158:162	arg1	node					164:167	Sentinel lymph node	149:167	Sentinel lymph node imaging	149:175	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	7	22	theme	imaging	1319:1325	arg1	ratio					1303:1307	the signal-to-background ratio	1278:1307	the signal-to-background ratio of NIR II imaging	1278:1325	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	2	23	theme	clinical	268:275	arg1	node					292:295	clinical sentinel lymph node	268:295	clinical sentinel lymph node imaging	268:303	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	5	24	theme	folic	774:778	arg1	acid					780:783	folic acid	774:783	folic acid	774:783	The coupled hyaluronic acid and folic acid can target to the tumor cells and dentritic cells with a dual-targeting effect.
37331158	8	25	theme	imaging	1466:1472	arg1	contrast					1454:1461	contrast	1454:1461	contrast	1454:1461	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	8	25	theme	imaging	1466:1472	arg1	depth					1444:1448	penetration depth	1432:1448	penetration depth	1432:1448	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	0	26	theme	sentinel	107:114	arg1	nodes					122:126	sentinel lymph nodes surgical navigation	107:146	sentinel lymph nodes surgical navigation	107:146	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	0	27	dep	nodes	122:126	arg1	navigation					137:146	surgical navigation	128:146	sentinel lymph nodes surgical navigation	107:146	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	5	28	theme	coupled	746:752	arg1	acid					765:768	The coupled hyaluronic acid	742:768	The coupled hyaluronic acid	742:768	The coupled hyaluronic acid and folic acid can target to the tumor cells and dentritic cells with a dual-targeting effect.
37331158	8	29	theme	retention	1489:1497	arg1	time					1499:1502	the retention time	1485:1502	the retention time	1485:1502	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	0	30	theme	luminescence	79:90	arg1	imaging					92:98	NIR II luminescence imaging	72:98	NIR II luminescence imaging	72:98	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	0	31	theme	Dual-targeting	0:13	arg1	nanoplatform					34:45	Dual-targeting lanthanide-ICG-MOF nanoplatform	0:45	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.	0:147	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	7	32	theme	MOF	1141:1143	arg1	carrier					1145:1151	the MOF carrier	1137:1151	the MOF carrier	1137:1151	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	7	33	theme	signal-to-background	1282:1301	arg1	ratio					1303:1307	the signal-to-background ratio	1278:1307	the signal-to-background ratio of NIR II imaging	1278:1325	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	3	34	theme	luminescence	434:445	arg1	technology					447:456	luminescence technology	434:456	luminescence technology	434:456	The combination of bio-conjugates chemistry and luminescence technology may achieve the specific targeting effect.
37331158	4	35	theme	metal-organic	585:597	arg1	MOF					610:612	MOF	610:612	MOF	610:612	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	4	35	theme	metal-organic	585:597	arg1	framework					599:607	a metal-organic framework	583:607	a metal-organic framework (MOF)	583:613	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	3	36	theme	targeting	483:491	arg1	effect					493:498	the specific targeting effect	470:498	the specific targeting effect	470:498	The combination of bio-conjugates chemistry and luminescence technology may achieve the specific targeting effect.
37331158	0	37	theme	cancer	51:56	arg1	Theranostics					58:69	cancer Theranostics	51:69	cancer Theranostics	51:69	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	6	38	theme	popliteal	1013:1021	arg1	nodes					1029:1033	normal popliteal lymph nodes	1006:1033	normal popliteal lymph nodes	1006:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	3	39	theme	chemistry	420:428	arg1	combination					390:400	The combination	386:400	The combination of bio-conjugates chemistry and luminescence technology	386:456	The combination of bio-conjugates chemistry and luminescence technology may achieve the specific targeting effect.
37331158	8	40	theme	sentinel	1522:1529	arg1	nodes					1537:1541	the sentinel lymph nodes	1518:1541	the sentinel lymph nodes surgical resection	1518:1560	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	8	41	theme	nodes	1537:1541	arg1	resection					1552:1560	the sentinel lymph nodes surgical resection	1518:1560	the sentinel lymph nodes surgical resection	1518:1560	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	8	42	theme	FA-HA/ICG	1386:1394	arg1	nanoplatform					1405:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	1	43	theme	metastasis	247:256	arg1	prediction					219:228	prediction	219:228	prediction of postoperative metastasis	219:256	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	1	43	theme	metastasis	247:256	arg1	staging					207:213	breast tumor staging	194:213	breast tumor staging	194:213	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	0	44	dep	nanoplatform	34:45	arg1	guided					100:105	guided	100:105	guided sentinel lymph nodes surgical navigation	100:146	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	8	45	theme	Ln	1396:1397	arg1	nanoplatform					1405:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	1	46	theme	breast	194:199	arg1	staging					207:213	breast tumor staging	194:213	breast tumor staging	194:213	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	6	47	theme	lymph	1081:1085	arg1	node					1087:1090	metastatic sentinel lymph node	1061:1090	metastatic sentinel lymph node from normal	1061:1102	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	2	48	theme	retention	370:378	arg1	time					380:383	short retention time	364:383	short retention time	364:383	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	9	49	theme	node	1611:1614	arg1	imaging					1616:1622	lymph node imaging	1605:1622	lymph node imaging	1605:1622	This study has important implications for lymph node imaging and surgical navigation.
37331158	7	50	theme	absorbed	1222:1229	arg1	energy					1242:1247	the absorbed excitation energy	1218:1247	the absorbed excitation energy from ICG	1218:1256	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	1	51	theme	tumor	201:205	arg1	staging					207:213	breast tumor staging	194:213	breast tumor staging	194:213	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	5	52	theme	dual-targeting	842:855	arg1	effect					857:862	a dual-targeting effect	840:862	a dual-targeting effect	840:862	The coupled hyaluronic acid and folic acid can target to the tumor cells and dentritic cells with a dual-targeting effect.
37331158	6	53	theme	@	880:880	arg1	nanoprobes					885:894	The FA-HA/ZIF-8@ICG nanoprobes	865:894	The FA-HA/ZIF-8@ICG nanoprobes	865:894	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	7	54	dep	in	1356:1357	arg1	vivo					1359:1362	vivo	1359:1362	vivo	1359:1362	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	2	55	theme	low	329:331	arg1	specificity					333:343	low specificity	329:343	low specificity	329:343	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	5	56	theme	dentritic	819:827	arg1	cells					829:833	dentritic cells	819:833	dentritic cells	819:833	The coupled hyaluronic acid and folic acid can target to the tumor cells and dentritic cells with a dual-targeting effect.
37331158	6	57	theme	stronger	949:956	arg1	times					986:990	1.6 times	982:990	1.6 times	982:990	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	6	57	theme	stronger	949:956	arg1	intensity					971:979	a stronger luminescence intensity	947:979	a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes	947:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	8	58	theme	ZIF-8	1399:1403	arg1	nanoplatform					1405:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	6	59	with	node	937:940	arg1	times					986:990	1.6 times	982:990	1.6 times	982:990	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	6	59	with	node	937:940	arg1	intensity					971:979	a stronger luminescence intensity	947:979	a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes	947:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	4	60	theme	dual-targeting	533:546	arg1	∼50 nm					569:574	∼50 nm	569:574	∼50 nm	569:574	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	4	60	theme	dual-targeting	533:546	arg1	nanoprobe					558:566	a dual-targeting composite nanoprobe	531:566	a dual-targeting composite nanoprobe (∼50 nm)	531:575	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	6	61	theme	lymph	931:935	arg1	node					937:940	sentinel lymph node	922:940	sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes	922:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	8	62	theme	penetration	1432:1442	arg1	depth					1444:1448	penetration depth	1432:1448	penetration depth	1432:1448	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	2	63	theme	node	292:295	arg1	imaging					297:303	clinical sentinel lymph node imaging	268:303	clinical sentinel lymph node imaging	268:303	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	1	64	theme	Sentinel	149:156	arg1	node					164:167	Sentinel lymph node	149:167	Sentinel lymph node imaging	149:175	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	6	65	theme	normal	1006:1011	arg1	nodes					1029:1033	normal popliteal lymph nodes	1006:1033	normal popliteal lymph nodes	1006:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	7	66	theme	long	1336:1339	arg1	time					1351:1354	long retention time	1336:1354	long retention time in vivo imaging	1336:1370	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	4	67	theme	metastatic	718:727	arg1	nodes					735:739	metastatic lymph nodes	718:739	metastatic lymph nodes	718:739	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	5	68	theme	hyaluronic	754:763	arg1	acid					765:768	The coupled hyaluronic acid	742:768	The coupled hyaluronic acid	742:768	The coupled hyaluronic acid and folic acid can target to the tumor cells and dentritic cells with a dual-targeting effect.
37331158	2	69	theme	sentinel	277:284	arg1	node					292:295	clinical sentinel lymph node	268:295	clinical sentinel lymph node imaging	268:303	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	1	70	theme	node	164:167	arg1	imaging					169:175	Sentinel lymph node imaging	149:175	Sentinel lymph node imaging	149:175	Sentinel lymph node imaging is important for breast tumor staging and prediction of postoperative metastasis.
37331158	7	71	theme	near-infrared	1184:1196	arg1	dye					1198:1200	near-infrared dye	1184:1200	near-infrared dye	1184:1200	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	4	72	theme	composite	548:556	arg1	∼50 nm					569:574	∼50 nm	569:574	∼50 nm	569:574	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	4	72	theme	composite	548:556	arg1	nanoprobe					558:566	a dual-targeting composite nanoprobe	531:566	a dual-targeting composite nanoprobe (∼50 nm)	531:575	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	4	73	theme	folic	697:701	arg1	acid					703:706	folic acid	697:706	folic acid	697:706	In this research, we designed a dual-targeting composite nanoprobe (∼50 nm) using a metal-organic framework (MOF) as carrier, loaded with lanthanide and ICG, and combined with hyaluronic acid and folic acid to detect metastatic lymph nodes.
37331158	0	74	theme	lymph	116:120	arg1	nodes					122:126	sentinel lymph nodes surgical navigation	107:146	sentinel lymph nodes surgical navigation	107:146	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	9	75	theme	important	1578:1586	arg1	implications					1588:1599	important implications	1578:1599	important implications for lymph node imaging and surgical navigation	1578:1646	This study has important implications for lymph node imaging and surgical navigation.
37331158	0	76	theme	surgical	128:135	arg1	navigation					137:146	surgical navigation	128:146	sentinel lymph nodes surgical navigation	107:146	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	7	77	theme	NIR	1312:1314	arg1	imaging					1319:1325	NIR II imaging	1312:1325	NIR II imaging	1312:1325	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	3	78	theme	specific	474:481	arg1	effect					493:498	the specific targeting effect	470:498	the specific targeting effect	470:498	The combination of bio-conjugates chemistry and luminescence technology may achieve the specific targeting effect.
37331158	0	79	theme	lanthanide-ICG-MOF	15:32	arg1	nanoplatform					34:45	Dual-targeting lanthanide-ICG-MOF nanoplatform	0:45	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.	0:147	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	3	80	theme	bio-conjugates	405:418	arg1	chemistry					420:428	bio-conjugates chemistry	405:428	bio-conjugates chemistry	405:428	The combination of bio-conjugates chemistry and luminescence technology may achieve the specific targeting effect.
37331158	2	81	contain	has	305:307	arg1	imaging					297:303	clinical sentinel lymph node imaging	268:303	clinical sentinel lymph node imaging	268:303	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	2	81	contain	has	305:307	arg2	time					380:383	short retention time	364:383	short retention time	364:383	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	2	81	contain	has	305:307	arg2	specificity					333:343	low specificity	329:343	low specificity	329:343	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	2	81	contain	has	305:307	arg2	contrast					350:357	low contrast	346:357	low contrast	346:357	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	2	81	contain	has	305:307	arg2	limitations					309:319	limitations	309:319	limitations such as low specificity, low contrast, and short retention time	309:383	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	6	82	theme	lymph	1023:1027	arg1	nodes					1029:1033	normal popliteal lymph nodes	1006:1033	normal popliteal lymph nodes	1006:1033	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	3	83	theme	technology	447:456	arg1	combination					390:400	The combination	386:400	The combination of bio-conjugates chemistry and luminescence technology	386:456	The combination of bio-conjugates chemistry and luminescence technology may achieve the specific targeting effect.
37331158	8	84	theme	lymph	1531:1535	arg1	nodes					1537:1541	the sentinel lymph nodes	1518:1541	the sentinel lymph nodes surgical resection	1518:1560	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	6	85	from	normal	1097:1102	arg1	node					1087:1090	metastatic sentinel lymph node	1061:1090	metastatic sentinel lymph node from normal	1061:1102	The FA-HA/ZIF-8@ICG nanoprobes can accumulate rapidly in sentinel lymph node with a stronger luminescence intensity (1.6 times) than that of normal popliteal lymph nodes in vivo, thus distinguish metastatic sentinel lymph node from normal effectively.
37331158	7	86	theme	integrated	1158:1167	arg1	lanthanide					1169:1178	the integrated lanthanide	1154:1178	the integrated lanthanide	1154:1178	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	0	87	theme	NIR	72:74	arg1	imaging					92:98	NIR II luminescence imaging	72:98	NIR II luminescence imaging	72:98	Dual-targeting lanthanide-ICG-MOF nanoplatform for cancer Theranostics: NIR II luminescence imaging guided sentinel lymph nodes surgical navigation.
37331158	8	88	theme	surgical	1543:1550	arg1	resection					1552:1560	the sentinel lymph nodes surgical resection	1518:1560	the sentinel lymph nodes surgical resection	1518:1560	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	2	89	theme	low	346:348	arg1	contrast					350:357	low contrast	346:357	low contrast	346:357	However, clinical sentinel lymph node imaging has limitations such as low specificity, low contrast, and short retention time.
37331158	7	90	theme	excitation	1231:1240	arg1	energy					1242:1247	the absorbed excitation energy	1218:1247	the absorbed excitation energy from ICG	1218:1256	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	8	91	theme	@	1395:1395	arg1	nanoplatform					1405:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	the FA-HA/ICG@Ln@ZIF-8 nanoplatform	1382:1416	Finally, the FA-HA/ICG@Ln@ZIF-8 nanoplatform increased the penetration depth and contrast of imaging, prolonged the retention time, and achieved the sentinel lymph nodes surgical resection.
37331158	7	92	contain	have	1331:1334	arg1	lanthanide					1169:1178	the integrated lanthanide	1154:1178	the integrated lanthanide	1154:1178	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
37331158	7	92	contain	have	1331:1334	arg2	time					1351:1354	long retention time	1336:1354	long retention time in vivo imaging	1336:1370	Furthermore, due to the MOF carrier, the integrated lanthanide and near-infrared dye by transferring the absorbed excitation energy from ICG to Nd3+ can enhance the signal-to-background ratio of NIR II imaging and have long retention time in vivo imaging.
36283781	9	0	theme	human	1438:1442	arg1	EPO					1460:1462	EPO	1460:1462	EPO	1460:1462	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	0	theme	human	1438:1442	arg1	erythropoietin					1444:1457	recombinant human erythropoietin	1426:1457	recombinant human erythropoietin (EPO)	1426:1463	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	1	link	O-linked	1553:1560	arg1	sialoglycopeptides					1562:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	8	2	theme	model	1276:1280	arg1	sialoglycopeptide					1282:1298	a selected model sialoglycopeptide	1265:1298	a selected model sialoglycopeptide	1265:1298	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	4	3	theme	sialic	889:894	arg1	acid					896:899	sialic acid	889:899	sialic acid	889:899	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	4	4	theme	novel	611:615	arg1	method					649:654	a novel derivatization-based enrichment method	609:654	a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid	609:899	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	5	5	gly	glycopeptides	958:970	arg2	glycopeptides					958:970	sialylated glycopeptides	947:970	sialylated glycopeptides	947:970	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	5	6	theme	glycopeptides	958:970	arg1	derivatization					929:942	derivatization	929:942	derivatization of sialylated glycopeptides	929:970	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	4	7	theme	acids	704:708	arg1	isomers					686:692	linkage isomers	678:692	linkage isomers of sialic acids	678:708	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	10	8	dep	simple	1596:1601	arg1	efficient					1604:1612	efficient	1604:1612	efficient	1604:1612	The method is simple, efficient, and targets small-scale sialoglycopeptide enrichment.
36283781	10	8	dep	simple	1596:1601	arg1	simple					1596:1601	simple	1596:1601	simple	1596:1601	The method is simple, efficient, and targets small-scale sialoglycopeptide enrichment.
36283781	10	8	dep	simple	1596:1601	arg1	method					1586:1591	The method	1582:1591	The method	1582:1591	The method is simple, efficient, and targets small-scale sialoglycopeptide enrichment.
36283781	8	9	gly	sialoglycopeptide	1282:1298	arg2	sialoglycopeptide					1282:1298	a selected model sialoglycopeptide	1265:1298	a selected model sialoglycopeptide	1265:1298	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	8	10	theme	recovery	1334:1341	arg1	%					1352:1352	suggesting recovery rate >100%	1323:1352	suggesting recovery rate >100%	1323:1352	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	8	10	theme	recovery	1334:1341	arg1	%					1320:1320	∼30%	1317:1320	∼30% (suggesting recovery rate >100%)	1317:1353	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	8	11	theme	suggesting	1323:1332	arg1	%					1352:1352	suggesting recovery rate >100%	1323:1352	suggesting recovery rate >100%	1323:1352	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	8	11	theme	suggesting	1323:1332	arg1	%					1320:1320	∼30%	1317:1320	∼30% (suggesting recovery rate >100%)	1317:1353	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	10	12	theme	small-scale	1627:1637	arg1	enrichment					1657:1666	small-scale sialoglycopeptide enrichment	1627:1666	small-scale sialoglycopeptide enrichment	1627:1666	The method is simple, efficient, and targets small-scale sialoglycopeptide enrichment.
36283781	9	13	theme	DOSG+ -based	1499:1510	arg1	enrichment					1516:1525	DOSG+ -based WCX enrichment	1499:1525	DOSG+ -based WCX enrichment	1499:1525	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	8	14	theme	rate	1343:1346	arg1	%					1352:1352	suggesting recovery rate >100%	1323:1352	suggesting recovery rate >100%	1323:1352	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	8	14	theme	rate	1343:1346	arg1	%					1320:1320	∼30%	1317:1320	∼30% (suggesting recovery rate >100%)	1317:1353	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	9	15	theme	enrichment	1516:1525	arg1	application					1484:1494	the application	1480:1494	the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides	1480:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	0	16	theme	sialylated	18:27	arg1	glycopeptides					29:41	sialylated glycopeptides	18:41	sialylated glycopeptides	18:41	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	9	17	theme	complexed	1530:1538	arg1	sialoglycopeptides					1562:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	0	18	theme	exchange	97:104	arg1	media					106:110	cation exchange media	90:110	cation exchange media	90:110	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	1	19	theme	important	146:154	arg1	modifications					175:187	the most important post-translational modifications	137:187	the most important post-translational modifications	137:187	Glycosylation is one of the most important post-translational modifications.
36283781	10	20	gly	sialoglycopeptide	1639:1655	arg2	sialoglycopeptide					1639:1655	small-scale sialoglycopeptide enrichment	1627:1666	small-scale sialoglycopeptide enrichment	1627:1666	The method is simple, efficient, and targets small-scale sialoglycopeptide enrichment.
36283781	2	21	theme	sialoglycopeptides	444:461	arg1	efficiency					430:439	the low ionization efficiency	411:439	the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups	411:496	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	2	21	theme	sialoglycopeptides	444:461	arg1	peptides					398:405	high abundant peptides	384:405	high abundant peptides	384:405	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	5	22	theme	electrostatic	1023:1035	arg1	interaction					1037:1047	electrostatic interaction	1023:1047	electrostatic interaction using weak cation exchange (WCX) media	1023:1086	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	7	23	dep	removed	1200:1206	arg1	%					1237:1237	recovery rate <1%	1221:1237	recovery rate <1%	1221:1237	Peptides were removed efficiently (recovery rate <1%).
36283781	4	24	theme	peptides	770:777	arg1	charge					760:765	the net charge	752:765	the net charge of peptides	752:777	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	4	25	gly	sialoglycopeptides	830:847	arg2	sialoglycopeptides					830:847	sialoglycopeptides	830:847	sialoglycopeptides	830:847	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	2	26	theme	carboxyl	482:489	arg1	groups					491:496	the carboxyl groups	478:496	the carboxyl groups	478:496	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	7	27	theme	recovery	1221:1228	arg1	%					1237:1237	recovery rate <1%	1221:1237	recovery rate <1%	1221:1237	Peptides were removed efficiently (recovery rate <1%).
36283781	4	28	dep	unify	746:750	arg1	ii					742:743	ii	742:743	ii	742:743	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	6	29	theme	DOSG+ -based	1089:1100	arg1	enrichment					1106:1115	DOSG+ -based WCX enrichment	1089:1115	DOSG+ -based WCX enrichment	1089:1115	DOSG+ -based WCX enrichment was validated and optimized with samples derived from bovine fetuin.
36283781	4	30	theme	derivatization-based	617:636	arg1	method					649:654	a novel derivatization-based enrichment method	609:654	a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid	609:899	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	6	31	attach	derived	1158:1164	arg1	fetuin					1178:1183	bovine fetuin	1171:1183	bovine fetuin	1171:1183	DOSG+ -based WCX enrichment was validated and optimized with samples derived from bovine fetuin.
36283781	6	31	attach	derived	1158:1164	arg2	samples					1150:1156	samples	1150:1156	samples derived from bovine fetuin	1150:1183	DOSG+ -based WCX enrichment was validated and optimized with samples derived from bovine fetuin.
36283781	4	32	theme	generating	713:722	arg1	difference					729:738	generating mass difference	713:738	generating mass difference	713:738	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	3	33	theme	efficient	540:548	arg1	method					561:566	an efficient enrichment method	537:566	an efficient enrichment method for sialoglycopeptides	537:589	Therefore, it is essential to develop an efficient enrichment method for sialoglycopeptides.
36283781	9	34	theme	alpha-1	1389:1395	arg1	AGP					1416:1418	AGP	1416:1418	AGP	1416:1418	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	34	theme	alpha-1	1389:1395	arg1	glycoprotein					1402:1413	human alpha-1 acid glycoprotein	1383:1413	human alpha-1 acid glycoprotein (AGP)	1383:1419	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	0	35	gly	glycopeptides	29:41	arg2	glycopeptides					29:41	sialylated glycopeptides	18:41	sialylated glycopeptides	18:41	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	1	36	theme	modifications	175:187	arg1	modifications					175:187	the most important post-translational modifications	137:187	the most important post-translational modifications	137:187	Glycosylation is one of the most important post-translational modifications.
36283781	1	36	theme	modifications	175:187	arg1	one					130:132	one	130:132	one	130:132	Glycosylation is one of the most important post-translational modifications.
36283781	2	37	theme	glycopeptides	224:236	arg1	characterizations					203:219	the characterizations	199:219	the characterizations of glycopeptides	199:236	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	2	37	theme	glycopeptides	224:236	arg1	sialoglycopeptides					273:290	the negatively charged sialoglycopeptides	250:290	the negatively charged sialoglycopeptides that are associated with various diseases	250:332	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	2	38	theme	low	415:417	arg1	efficiency					430:439	the low ionization efficiency	411:439	the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups	411:496	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	5	39	theme	cation	1060:1065	arg1	WCX					1077:1079	WCX	1077:1079	WCX	1077:1079	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	5	39	theme	cation	1060:1065	arg1	exchange					1067:1074	weak cation exchange	1055:1074	weak cation exchange (WCX) media	1055:1086	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	9	40	theme	recombinant	1426:1436	arg1	EPO					1460:1462	EPO	1460:1462	EPO	1460:1462	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	40	theme	recombinant	1426:1436	arg1	erythropoietin					1444:1457	recombinant human erythropoietin	1426:1457	recombinant human erythropoietin (EPO)	1426:1463	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	2	41	theme	abundant	389:396	arg1	peptides					398:405	high abundant peptides	384:405	high abundant peptides	384:405	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	4	42	theme	quaternary	864:873	arg1	ammonium					875:882	quaternary ammonium	864:882	quaternary ammonium	864:882	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	8	43	theme	selected	1267:1274	arg1	sialoglycopeptide					1282:1298	a selected model sialoglycopeptide	1265:1298	a selected model sialoglycopeptide	1265:1298	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	4	44	dep	introduce	800:808	arg1	iii					795:797	iii	795:797	iii	795:797	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	8	45	theme	sialoglycopeptide	1282:1298	arg1	intensity					1252:1260	The signal intensity	1241:1260	The signal intensity of a selected model sialoglycopeptide	1241:1298	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	5	46	gly	sialylated	947:956	arg1	glycopeptides					958:970	sialylated glycopeptides	947:970	sialylated glycopeptides	947:970	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	2	47	gly	sialoglycopeptides	273:290	arg2	characterizations					203:219	the characterizations	199:219	the characterizations of glycopeptides	199:236	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	2	47	gly	sialoglycopeptides	273:290	arg2	sialoglycopeptides					273:290	the negatively charged sialoglycopeptides	250:290	the negatively charged sialoglycopeptides that are associated with various diseases	250:332	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	2	48	theme	high	384:387	arg1	peptides					398:405	high abundant peptides	384:405	high abundant peptides	384:405	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	4	49	theme	mass	724:727	arg1	difference					729:738	generating mass difference	713:738	generating mass difference	713:738	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	8	50	theme	signal	1245:1250	arg1	intensity					1252:1260	The signal intensity	1241:1260	The signal intensity of a selected model sialoglycopeptide	1241:1298	The signal intensity of a selected model sialoglycopeptide was increased by ∼30% (suggesting recovery rate >100%).
36283781	4	51	theme	sialic	697:702	arg1	acids					704:708	sialic acids	697:708	sialic acids	697:708	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	10	52	theme	sialoglycopeptide	1639:1655	arg1	enrichment					1657:1666	small-scale sialoglycopeptide enrichment	1627:1666	small-scale sialoglycopeptide enrichment	1627:1666	The method is simple, efficient, and targets small-scale sialoglycopeptide enrichment.
36283781	2	53	theme	charged	265:271	arg1	sialoglycopeptides					273:290	the negatively charged sialoglycopeptides	250:290	the negatively charged sialoglycopeptides that are associated with various diseases	250:332	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	2	53	theme	charged	265:271	arg1	characterizations					203:219	the characterizations	199:219	the characterizations of glycopeptides	199:236	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	5	54	theme	sialylated	947:956	arg1	glycopeptides					958:970	sialylated glycopeptides	947:970	sialylated glycopeptides	947:970	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	4	55	dep	identify	669:676	arg1	i					666:666	i	666:666	i	666:666	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	2	56	theme	various	317:323	arg1	diseases					325:332	various diseases	317:332	various diseases	317:332	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	9	57	theme	WCX	1512:1514	arg1	enrichment					1516:1525	DOSG+ -based WCX enrichment	1499:1525	DOSG+ -based WCX enrichment	1499:1525	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	58	theme	O-linked	1553:1560	arg1	sialoglycopeptides					1562:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	4	59	theme	net	756:758	arg1	charge					760:765	the net charge	752:765	the net charge of peptides	752:777	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	2	60	gly	glycopeptides	224:236	arg2	glycopeptides					224:236	glycopeptides	224:236	glycopeptides	224:236	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	0	61	theme	cation	90:95	arg1	media					106:110	cation exchange media	90:110	cation exchange media	90:110	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	1	62	theme	post-translational	156:173	arg1	modifications					175:187	the most important post-translational modifications	137:187	the most important post-translational modifications	137:187	Glycosylation is one of the most important post-translational modifications.
36283781	9	63	theme	N-linked	1540:1547	arg1	sialoglycopeptides					1562:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	5	64	theme	efficient	994:1002	arg1	enrichment					1004:1013	efficient enrichment	994:1013	efficient enrichment	994:1013	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	3	65	gly	sialoglycopeptides	572:589	arg2	sialoglycopeptides					572:589	sialoglycopeptides	572:589	sialoglycopeptides	572:589	Therefore, it is essential to develop an efficient enrichment method for sialoglycopeptides.
36283781	2	66	gly	sialoglycopeptides	444:461	arg2	sialoglycopeptides					444:461	sialoglycopeptides	444:461	sialoglycopeptides resulting from the carboxyl groups	444:496	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	7	67	theme	rate	1230:1233	arg1	%					1237:1237	recovery rate <1%	1221:1237	recovery rate <1%	1221:1237	Peptides were removed efficiently (recovery rate <1%).
36283781	9	68	gly	sialoglycopeptides	1562:1579	arg2	sialoglycopeptides					1562:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	2	69	theme	ionization	419:428	arg1	efficiency					430:439	the low ionization efficiency	411:439	the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups	411:496	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	0	70	theme	glycopeptides	29:41	arg1	Derivatization					0:13	Derivatization	0:13	Derivatization of sialylated glycopeptides plus based	0:52	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	0	71	gly	sialoglycopeptides	54:71	arg2	sialoglycopeptides					54:71	sialoglycopeptides	54:71	sialoglycopeptides enrichment using cation exchange media	54:110	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	9	72	link	N-linked	1540:1547	arg1	sialoglycopeptides					1562:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	6	73	theme	WCX	1102:1104	arg1	enrichment					1106:1115	DOSG+ -based WCX enrichment	1089:1115	DOSG+ -based WCX enrichment	1089:1115	DOSG+ -based WCX enrichment was validated and optimized with samples derived from bovine fetuin.
36283781	4	74	theme	enrichment	638:647	arg1	method					649:654	a novel derivatization-based enrichment method	609:654	a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid	609:899	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	0	75	theme	based	48:52	arg1	Derivatization					0:13	Derivatization	0:13	Derivatization of sialylated glycopeptides plus based	0:52	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	2	76	with	co-existence	366:377	arg1	efficiency					430:439	the low ionization efficiency	411:439	the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups	411:496	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	2	76	with	co-existence	366:377	arg1	peptides					398:405	high abundant peptides	384:405	high abundant peptides	384:405	However, the characterizations of glycopeptides, especially the negatively charged sialoglycopeptides that are associated with various diseases, remain challenging, due to the co-existence with high abundant peptides and the low ionization efficiency of sialoglycopeptides resulting from the carboxyl groups.
36283781	5	77	theme	exchange	1067:1074	arg1	media					1082:1086	weak cation exchange (WCX) media	1055:1086	weak cation exchange (WCX) media	1055:1086	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	9	78	gly	glycoprotein	1402:1413	arg1	AGP					1416:1418	AGP	1416:1418	AGP	1416:1418	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	78	gly	glycoprotein	1402:1413	arg1	glycoprotein					1402:1413	human alpha-1 acid glycoprotein	1383:1413	human alpha-1 acid glycoprotein (AGP)	1383:1419	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	79	theme	human	1383:1387	arg1	AGP					1416:1418	AGP	1416:1418	AGP	1416:1418	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	79	theme	human	1383:1387	arg1	glycoprotein					1402:1413	human alpha-1 acid glycoprotein	1383:1413	human alpha-1 acid glycoprotein (AGP)	1383:1419	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	0	80	gly	sialylated	18:27	arg1	glycopeptides					29:41	sialylated glycopeptides	18:41	sialylated glycopeptides	18:41	Derivatization of sialylated glycopeptides plus based sialoglycopeptides enrichment using cation exchange media.
36283781	9	81	from	application	1484:1494	arg1	sialoglycopeptides					1562:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	complexed N-linked and O-linked sialoglycopeptides	1530:1579	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	3	82	theme	enrichment	550:559	arg1	method					561:566	an efficient enrichment method	537:566	an efficient enrichment method for sialoglycopeptides	537:589	Therefore, it is essential to develop an efficient enrichment method for sialoglycopeptides.
36283781	9	83	theme	acid	1397:1400	arg1	AGP					1416:1418	AGP	1416:1418	AGP	1416:1418	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	9	83	theme	acid	1397:1400	arg1	glycoprotein					1402:1413	human alpha-1 acid glycoprotein	1383:1413	human alpha-1 acid glycoprotein (AGP)	1383:1419	The method was employed on human alpha-1 acid glycoprotein (AGP), and recombinant human erythropoietin (EPO), demonstrating the application of DOSG+ -based WCX enrichment on complexed N-linked and O-linked sialoglycopeptides.
36283781	6	84	theme	bovine	1171:1176	arg1	fetuin					1178:1183	bovine fetuin	1171:1183	bovine fetuin	1171:1183	DOSG+ -based WCX enrichment was validated and optimized with samples derived from bovine fetuin.
36283781	5	85	theme	weak	1055:1058	arg1	WCX					1077:1079	WCX	1077:1079	WCX	1077:1079	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	5	85	theme	weak	1055:1058	arg1	exchange					1067:1074	weak cation exchange	1055:1074	weak cation exchange (WCX) media	1055:1086	The derivatization, termed derivatization of sialylated glycopeptides plus (DOSG+), enables efficient enrichment through electrostatic interaction using weak cation exchange (WCX) media.
36283781	4	86	theme	linkage	678:684	arg1	isomers					686:692	linkage isomers	678:692	linkage isomers of sialic acids	678:708	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36283781	4	87	theme	positive	810:817	arg1	charges					819:825	positive charges	810:825	positive charges to sialoglycopeptides	810:847	Here, we present a novel derivatization-based enrichment method that can (i) identify linkage isomers of sialic acids by generating mass difference, (ii) unify the net charge of peptides into zero, and (iii) introduce positive charges to sialoglycopeptides by conjugating quaternary ammonium with sialic acid.
36175956	1	0	theme	molecular	300:308	arg1	mechanisms					310:319	the underlying cellular and molecular mechanisms	272:319	the underlying cellular and molecular mechanisms	272:319	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	7	1	theme	gut-brain	1411:1419	arg1	axis					1421:1424	the gut-brain axis	1407:1424	the gut-brain axis	1407:1424	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	1	2	theme	receptor	155:162	arg1	lack					138:141	the lack	134:141	the lack of estrogen receptor β (ERβ)	134:170	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	1	2	theme	receptor	155:162	arg1	factor					182:187	a risk factor	175:187	a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders	175:269	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	5	3	theme	DSS	1046:1048	arg1	insult					1050:1055	DSS insult	1046:1055	DSS insult	1046:1055	In addition, RNA sequencing data suggested that ErbB4 might be the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult.
36175956	1	4	theme	bowel	225:229	arg1	disease					231:237	inflammatory bowel disease	212:237	inflammatory bowel disease (IBD)	212:243	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	1	4	theme	bowel	225:229	arg1	IBD					240:242	IBD	240:242	IBD	240:242	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	2	5	theme	anxiety-like	432:443	arg1	behavior					445:452	related anxiety-like behavior	424:452	related anxiety-like behavior in ERβ-deficient mice	424:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	2	6	from	development	401:411	arg1	mice					471:474	ERβ-deficient mice	457:474	ERβ-deficient mice	457:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	5	7	theme	axis	1017:1020	arg1	hyperactivity					1022:1034	the HPA axis hyperactivity	1009:1034	the HPA axis hyperactivity caused by DSS insult	1009:1055	In addition, RNA sequencing data suggested that ErbB4 might be the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult.
36175956	1	8	theme	disease	231:237	arg1	development					197:207	the development	193:207	the development of inflammatory bowel disease (IBD) and psychiatric disorders	193:269	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	0	9	theme	anxiety-like	91:102	arg1	behavior					104:111	IBD-induced anxiety-like behavior	79:111	IBD-induced anxiety-like behavior	79:111	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	3	10	theme	microbiota	621:630	arg1	composition					632:642	the fecal microbiota composition	611:642	the fecal microbiota composition	611:642	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	7	11	theme	anxiety-like	1346:1357	arg1	behaviors					1359:1367	anxiety-like behaviors	1346:1367	anxiety-like behaviors	1346:1367	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	3	12	theme	dextran	500:506	arg1	DSS					524:526	DSS	524:526	DSS	524:526	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	3	12	theme	dextran	500:506	arg1	sulfate					515:521	dextran sodium sulfate	500:521	dextran sodium sulfate (DSS) insult	500:534	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	8	13	theme	IBD	1636:1638	arg1	treatment					1623:1631	the treatment	1619:1631	the treatment of IBD and related anxiety-like behaviors	1619:1673	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	0	14	theme	IBD-induced	79:89	arg1	behavior					104:111	IBD-induced anxiety-like behavior	79:111	IBD-induced anxiety-like behavior	79:111	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	3	15	theme	fecal	615:619	arg1	composition					632:642	the fecal microbiota composition	611:642	the fecal microbiota composition	611:642	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	6	16	theme	anxiety-like	1131:1142	arg1	behaviors					1144:1152	anxiety-like behaviors	1131:1152	anxiety-like behaviors	1131:1152	Gut microbiota remodeling by co-housing showed that both the colitis and anxiety-like behaviors were aggravated in co-housed wild-type mice compared to single-housed wild-type mice.
36175956	3	17	from	RESULTS	477:483	arg1	response					488:495	response	488:495	response to dextran sodium sulfate (DSS) insult	488:534	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	3	18	theme	sodium	508:513	arg1	DSS					524:526	DSS	524:526	DSS	524:526	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	3	18	theme	sodium	508:513	arg1	sulfate					515:521	dextran sodium sulfate	500:521	dextran sodium sulfate (DSS) insult	500:534	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	3	19	theme	colitis	674:680	arg1	worsening					661:669	worsening	661:669	worsening of colitis and anxiety-like behaviors	661:707	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	1	20	dep	BACKGROUND	114:123	arg1	lack					138:141	the lack	134:141	the lack of estrogen receptor β (ERβ)	134:170	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	1	20	dep	BACKGROUND	114:123	arg1	factor					182:187	a risk factor	175:187	a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders	175:269	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	7	21	theme	colitis	1317:1323	arg1	activity					1333:1340	colitis disease activity	1317:1340	colitis disease activity	1317:1340	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	4	22	dep	hypothalamic-pituitary-adrenal	756:785	arg1	HPA					788:790	HPA	788:790	HPA	788:790	In addition, DSS-induced colitis also induced hypothalamic-pituitary-adrenal (HPA) axis hyperactivity in ERβ-deficient mice, which was associated with colitis and anxiety-like behaviors.
36175956	0	23	theme	behavior	104:111	arg1	mechanism					66:74	a possible mechanism	55:74	a possible mechanism of IBD-induced anxiety-like behavior	55:111	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	8	24	theme	gut	1541:1543	arg1	microbiota					1545:1554	the gut microbiota	1537:1554	the gut microbiota	1537:1554	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	2	25	theme	related	424:430	arg1	behavior					445:452	related anxiety-like behavior	424:452	related anxiety-like behavior in ERβ-deficient mice	424:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	0	26	theme	receptor	9:16	arg1	deficiency					20:29	Estrogen receptor β deficiency	0:29	Estrogen receptor β deficiency	0:29	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	3	27	theme	anxiety-like	686:697	arg1	behaviors					699:707	anxiety-like behaviors	686:707	anxiety-like behaviors	686:707	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	6	28	theme	wild-type	1224:1232	arg1	mice					1234:1237	single-housed wild-type mice	1210:1237	single-housed wild-type mice	1210:1237	Gut microbiota remodeling by co-housing showed that both the colitis and anxiety-like behaviors were aggravated in co-housed wild-type mice compared to single-housed wild-type mice.
36175956	2	29	theme	ERβ-deficient	457:469	arg1	mice					471:474	ERβ-deficient mice	457:474	ERβ-deficient mice	457:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	7	30	theme	critical	1290:1297	arg1	role					1299:1302	a critical role	1288:1302	a critical role	1288:1302	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	0	31	theme	Estrogen	0:7	arg1	deficiency					20:29	Estrogen receptor β deficiency	0:29	Estrogen receptor β deficiency	0:29	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	3	32	theme	behaviors	699:707	arg1	worsening					661:669	worsening	661:669	worsening of colitis and anxiety-like behaviors	661:707	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	4	33	theme	ERβ-deficient	815:827	arg1	mice					829:832	ERβ-deficient mice	815:832	ERβ-deficient mice	815:832	In addition, DSS-induced colitis also induced hypothalamic-pituitary-adrenal (HPA) axis hyperactivity in ERβ-deficient mice, which was associated with colitis and anxiety-like behaviors.
36175956	5	34	theme	RNA	910:912	arg1	data					925:928	RNA sequencing data	910:928	RNA sequencing data	910:928	In addition, RNA sequencing data suggested that ErbB4 might be the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult.
36175956	4	35	theme	hypothalamic-pituitary-adrenal	756:785	arg1	hyperactivity					798:810	hypothalamic-pituitary-adrenal (HPA) axis hyperactivity	756:810	hypothalamic-pituitary-adrenal (HPA) axis hyperactivity	756:810	In addition, DSS-induced colitis also induced hypothalamic-pituitary-adrenal (HPA) axis hyperactivity in ERβ-deficient mice, which was associated with colitis and anxiety-like behaviors.
36175956	6	36	theme	wild-type	1183:1191	arg1	mice					1193:1196	co-housed wild-type mice	1173:1196	co-housed wild-type mice	1173:1196	Gut microbiota remodeling by co-housing showed that both the colitis and anxiety-like behaviors were aggravated in co-housed wild-type mice compared to single-housed wild-type mice.
36175956	3	37	theme	sulfate	515:521	arg1	insult					529:534	dextran sodium sulfate (DSS) insult	500:534	dextran sodium sulfate (DSS) insult	500:534	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	2	38	theme	gut	379:381	arg1	microbiota					383:392	gut microbiota	379:392	gut microbiota	379:392	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	0	39	theme	β	18:18	arg1	deficiency					20:29	Estrogen receptor β deficiency	0:29	Estrogen receptor β deficiency	0:29	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	1	40	theme	psychiatric	249:259	arg1	disorders					261:269	psychiatric disorders	249:269	psychiatric disorders	249:269	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	4	41	theme	DSS-induced	723:733	arg1	colitis					735:741	DSS-induced colitis	723:741	DSS-induced colitis	723:741	In addition, DSS-induced colitis also induced hypothalamic-pituitary-adrenal (HPA) axis hyperactivity in ERβ-deficient mice, which was associated with colitis and anxiety-like behaviors.
36175956	2	42	from	behavior	445:452	arg1	mice					471:474	ERβ-deficient mice	457:474	ERβ-deficient mice	457:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	1	43	theme	risk	177:180	arg1	lack					138:141	the lack	134:141	the lack of estrogen receptor β (ERβ)	134:170	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	1	43	theme	risk	177:180	arg1	factor					182:187	a risk factor	175:187	a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders	175:269	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	0	44	theme	gut	39:41	arg1	microbiota					43:52	gut microbiota	39:52	gut microbiota	39:52	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	1	45	theme	disorders	261:269	arg1	development					197:207	the development	193:207	the development of inflammatory bowel disease (IBD) and psychiatric disorders	193:269	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	9	46	dep	Abstract	1682:1689	arg1	Video					1676:1680	Video	1676:1680	Video	1676:1680	Video Abstract.
36175956	8	47	theme	colitis	1472:1478	arg1	development					1480:1490	colitis development	1472:1490	colitis development	1472:1490	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	5	48	theme	ERβ	974:976	arg1	target					964:969	the target	960:969	the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult	960:1055	In addition, RNA sequencing data suggested that ErbB4 might be the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult.
36175956	5	48	theme	ERβ	974:976	arg1	ErbB4					945:949	ErbB4	945:949	ErbB4	945:949	In addition, RNA sequencing data suggested that ErbB4 might be the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult.
36175956	7	49	theme	gut	1268:1270	arg1	microbiota					1272:1281	gut microbiota	1268:1281	gut microbiota	1268:1281	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	7	50	theme	neural	1382:1387	arg1	processing					1389:1398	aberrant neural processing	1373:1398	aberrant neural processing within the gut-brain axis	1373:1424	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	3	51	theme	ERβ	541:543	arg1	mice					554:557	the ERβ knockout mice	537:557	the ERβ knockout mice	537:557	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	2	52	theme	IBD	416:418	arg1	development					401:411	the development	397:411	the development of IBD	397:418	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	2	52	theme	IBD	416:418	arg1	behavior					445:452	related anxiety-like behavior	424:452	related anxiety-like behavior in ERβ-deficient mice	424:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	3	53	from	shift	581:585	arg1	α					590:590	α	590:590	α	590:590	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	3	53	from	shift	581:585	arg1	diversity					598:606	β diversity	596:606	β diversity	596:606	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	3	53	from	shift	581:585	arg1	composition					632:642	the fecal microbiota composition	611:642	the fecal microbiota composition	611:642	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	8	54	theme	microbiota	1545:1554	arg1	remodeling					1523:1532	remodeling	1523:1532	remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors	1523:1673	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	4	55	theme	axis	793:796	arg1	hyperactivity					798:810	hypothalamic-pituitary-adrenal (HPA) axis hyperactivity	756:810	hypothalamic-pituitary-adrenal (HPA) axis hyperactivity	756:810	In addition, DSS-induced colitis also induced hypothalamic-pituitary-adrenal (HPA) axis hyperactivity in ERβ-deficient mice, which was associated with colitis and anxiety-like behaviors.
36175956	6	56	theme	single-housed	1210:1222	arg1	mice					1234:1237	single-housed wild-type mice	1210:1237	single-housed wild-type mice	1210:1237	Gut microbiota remodeling by co-housing showed that both the colitis and anxiety-like behaviors were aggravated in co-housed wild-type mice compared to single-housed wild-type mice.
36175956	1	57	theme	underlying	276:285	arg1	mechanisms					310:319	the underlying cellular and molecular mechanisms	272:319	the underlying cellular and molecular mechanisms	272:319	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	0	58	dep	impairs	31:37	arg1	mechanism					66:74	a possible mechanism	55:74	a possible mechanism of IBD-induced anxiety-like behavior	55:111	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	7	59	theme	disease	1325:1331	arg1	activity					1333:1340	colitis disease activity	1317:1340	colitis disease activity	1317:1340	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	8	60	theme	related	1644:1650	arg1	behaviors					1665:1673	related anxiety-like behaviors	1644:1673	related anxiety-like behaviors	1644:1673	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	1	61	theme	cellular	287:294	arg1	mechanisms					310:319	the underlying cellular and molecular mechanisms	272:319	the underlying cellular and molecular mechanisms	272:319	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	4	62	theme	anxiety-like	873:884	arg1	behaviors					886:894	anxiety-like behaviors	873:894	anxiety-like behaviors	873:894	In addition, DSS-induced colitis also induced hypothalamic-pituitary-adrenal (HPA) axis hyperactivity in ERβ-deficient mice, which was associated with colitis and anxiety-like behaviors.
36175956	6	63	theme	co-housed	1173:1181	arg1	mice					1193:1196	co-housed wild-type mice	1173:1196	co-housed wild-type mice	1173:1196	Gut microbiota remodeling by co-housing showed that both the colitis and anxiety-like behaviors were aggravated in co-housed wild-type mice compared to single-housed wild-type mice.
36175956	7	64	theme	aberrant	1373:1380	arg1	processing					1389:1398	aberrant neural processing	1373:1398	aberrant neural processing within the gut-brain axis	1373:1424	These findings suggest that gut microbiota play a critical role in mediating colitis disease activity and anxiety-like behaviors via aberrant neural processing within the gut-brain axis.
36175956	5	65	theme	sequencing	914:923	arg1	data					925:928	RNA sequencing data	910:928	RNA sequencing data	910:928	In addition, RNA sequencing data suggested that ErbB4 might be the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult.
36175956	8	66	theme	therapeutic	1596:1606	arg1	target					1608:1613	a promising therapeutic target	1584:1613	a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors	1584:1673	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	8	66	theme	therapeutic	1596:1606	arg1	ERβ					1577:1579	ERβ	1577:1579	ERβ	1577:1579	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	3	67	theme	β	596:596	arg1	diversity					598:606	β diversity	596:606	β diversity	596:606	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	3	68	theme	significant	569:579	arg1	shift					581:585	significant shift	569:585	significant shift in α and β diversity in the fecal microbiota composition	569:642	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	2	69	from	role	371:374	arg1	development					401:411	the development	397:411	the development of IBD	397:418	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	2	69	from	role	371:374	arg1	behavior					445:452	related anxiety-like behavior	424:452	related anxiety-like behavior in ERβ-deficient mice	424:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	6	70	theme	microbiota	1062:1071	arg1	remodeling					1073:1082	Gut microbiota remodeling	1058:1082	Gut microbiota remodeling by co-housing	1058:1096	Gut microbiota remodeling by co-housing showed that both the colitis and anxiety-like behaviors were aggravated in co-housed wild-type mice compared to single-housed wild-type mice.
36175956	8	71	theme	behaviors	1665:1673	arg1	treatment					1623:1631	the treatment	1619:1631	the treatment of IBD and related anxiety-like behaviors	1619:1673	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	8	72	theme	promising	1586:1594	arg1	target					1608:1613	a promising therapeutic target	1584:1613	a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors	1584:1673	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	8	72	theme	promising	1586:1594	arg1	ERβ					1577:1579	ERβ	1577:1579	ERβ	1577:1579	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	0	73	theme	possible	57:64	arg1	mechanism					66:74	a possible mechanism	55:74	a possible mechanism of IBD-induced anxiety-like behavior	55:111	Estrogen receptor β deficiency impairs gut microbiota: a possible mechanism of IBD-induced anxiety-like behavior.
36175956	5	74	theme	HPA	1013:1015	arg1	hyperactivity					1022:1034	the HPA axis hyperactivity	1009:1034	the HPA axis hyperactivity caused by DSS insult	1009:1055	In addition, RNA sequencing data suggested that ErbB4 might be the target of ERβ that is involved in regulating the HPA axis hyperactivity caused by DSS insult.
36175956	3	75	theme	knockout	545:552	arg1	mice					554:557	the ERβ knockout mice	537:557	the ERβ knockout mice	537:557	RESULTS In response to dextran sodium sulfate (DSS) insult, the ERβ knockout mice displayed significant shift in α and β diversity in the fecal microbiota composition and demonstrated worsening of colitis and anxiety-like behaviors.
36175956	6	76	theme	Gut	1058:1060	arg1	remodeling					1073:1082	Gut microbiota remodeling	1058:1082	Gut microbiota remodeling by co-housing	1058:1096	Gut microbiota remodeling by co-housing showed that both the colitis and anxiety-like behaviors were aggravated in co-housed wild-type mice compared to single-housed wild-type mice.
36175956	8	77	theme	anxiety-like	1652:1663	arg1	behaviors					1665:1673	related anxiety-like behaviors	1644:1673	related anxiety-like behaviors	1644:1673	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	8	78	theme	anxiety-like	1496:1507	arg1	behaviors					1509:1517	anxiety-like behaviors	1496:1517	anxiety-like behaviors	1496:1517	CONCLUSIONS ERβ has the potential to inhibit colitis development and anxiety-like behaviors via remodeling of the gut microbiota, which suggests that ERβ is a promising therapeutic target for the treatment of IBD and related anxiety-like behaviors.
36175956	2	79	theme	microbiota	383:392	arg1	role					371:374	the role	367:374	the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice	367:474	Herein, we revealed the role of gut microbiota in the development of IBD and related anxiety-like behavior in ERβ-deficient mice.
36175956	1	80	theme	estrogen	146:153	arg1	receptor					155:162	estrogen receptor β	146:164	estrogen receptor β (ERβ)	146:170	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	1	80	theme	estrogen	146:153	arg1	ERβ					167:169	ERβ	167:169	ERβ	167:169	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	1	81	theme	inflammatory	212:223	arg1	disease					231:237	inflammatory bowel disease	212:237	inflammatory bowel disease (IBD)	212:243	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36175956	1	81	theme	inflammatory	212:223	arg1	IBD					240:242	IBD	240:242	IBD	240:242	BACKGROUND Although the lack of estrogen receptor β (ERβ) is a risk factor for the development of inflammatory bowel disease (IBD) and psychiatric disorders, the underlying cellular and molecular mechanisms are not fully understood.
36633947	3	0	theme	sites	568:572	arg1	identification					509:522	identification	509:522	identification of the protein substrates and glycosylation sites of single GalNAc-Ts	509:592	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	0	1	theme	protein	99:105	arg1	substrates					107:116	their protein substrates	93:116	their protein substrates	93:116	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	4	2	theme	engineered	644:653	arg1	transferases					655:666	the engineered transferases	640:666	the engineered transferases	640:666	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	0	3	theme	glycosylation	122:134	arg1	sites					136:140	glycosylation sites	122:140	glycosylation sites in cells	122:149	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	5	4	from	details	792:798	arg1	execution					815:823	execution	815:823	execution	815:823	For complete details on the use and execution of this protocol, please refer to Schumann et al. (2020)1, Cioce et al. (2021)2, and Cioce et al. (2022)3.
36633947	5	4	from	details	792:798	arg1	use					807:809	use	807:809	use	807:809	For complete details on the use and execution of this protocol, please refer to Schumann et al. (2020)1, Cioce et al. (2021)2, and Cioce et al. (2022)3.
36633947	3	5	gly	glycosylation	554:566	arg2	GalNAc-Ts					584:592	single GalNAc-Ts	577:592	single GalNAc-Ts	577:592	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	3	5	gly	glycosylation	554:566	arg2	sites					568:572	glycosylation sites	554:572	glycosylation sites	554:572	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	3	5	gly	glycosylation	554:566	arg1	GalNAc-Ts					584:592	single GalNAc-Ts	577:592	single GalNAc-Ts	577:592	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	3	6	dep	substrates	539:548	arg1	the					527:529	the	527:529	the	527:529	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	5	7	dep	et al	868:872	arg1	3					929:929	3	929:929	3	929:929	For complete details on the use and execution of this protocol, please refer to Schumann et al. (2020)1, Cioce et al. (2021)2, and Cioce et al. (2022)3.
36633947	4	8	theme	substrates	714:723	arg1	biosynthesis					684:695	biosynthesis	684:695	biosynthesis of bioorthogonal substrates	684:723	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	4	9	theme	molecular	760:768	arg1	biology					770:776	molecular biology	760:776	molecular biology	760:776	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	2	10	theme	glycosyltransferases	427:446	arg1	family					417:422	the GalNAc-T family	404:422	the GalNAc-T family of glycosyltransferases	404:446	Here, we describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases using a tactic termed bump-and-hole engineering.
36633947	4	11	from	methods	749:755	arg1	biology					770:776	molecular biology	760:776	molecular biology	760:776	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	3	12	theme	GalNAc-Ts	584:592	arg1	substrates					539:548	protein substrates	531:548	protein substrates	531:548	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	3	12	theme	GalNAc-Ts	584:592	arg1	sites					568:572	glycosylation sites	554:572	glycosylation sites	554:572	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	3	12	theme	GalNAc-Ts	584:592	arg1	GalNAc-Ts					584:592	single GalNAc-Ts	577:592	single GalNAc-Ts	577:592	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	2	13	theme	chemical	350:357	arg1	reporters					374:382	chemical, bioorthogonal reporters	350:382	chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases	350:446	Here, we describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases using a tactic termed bump-and-hole engineering.
36633947	2	14	theme	family	417:422	arg1	activity					392:399	the activity	388:399	the activity of the GalNAc-T family of glycosyltransferases	388:446	Here, we describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases using a tactic termed bump-and-hole engineering.
36633947	0	15	theme	Bump-and-hole	0:12	arg1	engineering					14:24	Bump-and-hole engineering	0:24	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases	0:80	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	4	16	theme	cells	629:633	arg1	transfection					613:624	transfection	613:624	transfection of cells with the engineered transferases and enzymes	613:678	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	0	17	gly	glycosylation	122:134	arg2	sites					136:140	glycosylation sites	122:140	glycosylation sites in cells	122:149	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	1	18	theme	biological	204:213	arg1	function					215:222	biological function	204:222	biological function	204:222	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	0	19	theme	human	29:33	arg1	transferases					69:80	human polypeptide N-acetylgalactosamine transferases	29:80	human polypeptide N-acetylgalactosamine transferases	29:80	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	2	20	theme	bump-and-hole	470:482	arg1	engineering					484:494	bump-and-hole engineering	470:494	bump-and-hole engineering	470:494	Here, we describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases using a tactic termed bump-and-hole engineering.
36633947	0	21	from	substrates	107:116	arg1	cells					145:149	cells	145:149	cells	145:149	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	3	22	theme	single	577:582	arg1	GalNAc-Ts					584:592	single GalNAc-Ts	577:592	single GalNAc-Ts	577:592	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	0	23	theme	N-acetylgalactosamine	47:67	arg1	transferases					69:80	human polypeptide N-acetylgalactosamine transferases	29:80	human polypeptide N-acetylgalactosamine transferases	29:80	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	2	24	theme	GalNAc-T	408:415	arg1	family					417:422	the GalNAc-T family	404:422	the GalNAc-T family of glycosyltransferases	404:446	Here, we describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases using a tactic termed bump-and-hole engineering.
36633947	1	25	theme	substrate	228:236	arg1	specificities					238:250	substrate specificities	228:250	substrate specificities	228:250	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	1	26	theme	glycans	191:197	arg1	relevance					178:186	the known disease relevance	160:186	the known disease relevance of glycans	160:197	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	0	27	theme	polypeptide	35:45	arg1	transferases					69:80	human polypeptide N-acetylgalactosamine transferases	29:80	human polypeptide N-acetylgalactosamine transferases	29:80	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	4	28	theme	bioorthogonal	700:712	arg1	substrates					714:723	bioorthogonal substrates	700:723	bioorthogonal substrates	700:723	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	0	29	theme	transferases	69:80	arg1	engineering					14:24	Bump-and-hole engineering	0:24	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases	0:80	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	3	30	theme	protein	531:537	arg1	substrates					539:548	protein substrates	531:548	protein substrates	531:548	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	3	31	theme	glycosylation	554:566	arg1	sites					568:572	glycosylation sites	554:572	glycosylation sites	554:572	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	3	31	theme	glycosylation	554:566	arg1	GalNAc-Ts					584:592	single GalNAc-Ts	577:592	single GalNAc-Ts	577:592	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	0	32	from	sites	136:140	arg1	cells					145:149	cells	145:149	cells	145:149	Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
36633947	2	33	dep	chemical	350:357	arg1	bioorthogonal					360:372	bioorthogonal	360:372	bioorthogonal	360:372	Here, we describe a protocol to develop chemical, bioorthogonal reporters for the activity of the GalNAc-T family of glycosyltransferases using a tactic termed bump-and-hole engineering.
36633947	5	34	theme	complete	783:790	arg1	details					792:798	complete details	783:798	complete details on the use and execution of this protocol	783:840	For complete details on the use and execution of this protocol, please refer to Schumann et al. (2020)1, Cioce et al. (2021)2, and Cioce et al. (2022)3.
36633947	3	35	theme	substrates	539:548	arg1	identification					509:522	identification	509:522	identification of the protein substrates and glycosylation sites of single GalNAc-Ts	509:592	This allows identification of the protein substrates and glycosylation sites of single GalNAc-Ts.
36633947	1	36	theme	individual	255:264	arg1	glycosyltransferases					266:285	individual glycosyltransferases	255:285	individual glycosyltransferases	255:285	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	5	37	theme	protocol	833:840	arg1	execution					815:823	execution	815:823	execution	815:823	For complete details on the use and execution of this protocol, please refer to Schumann et al. (2020)1, Cioce et al. (2021)2, and Cioce et al. (2022)3.
36633947	5	37	theme	protocol	833:840	arg1	use					807:809	use	807:809	use	807:809	For complete details on the use and execution of this protocol, please refer to Schumann et al. (2020)1, Cioce et al. (2021)2, and Cioce et al. (2022)3.
36633947	4	38	with	transfection	613:624	arg1	enzymes					672:678	enzymes	672:678	enzymes	672:678	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	4	38	with	transfection	613:624	arg1	transferases					655:666	the engineered transferases	640:666	the engineered transferases	640:666	Despite requiring transfection of cells with the engineered transferases and enzymes for biosynthesis of bioorthogonal substrates, the tactic complements methods in molecular biology.
36633947	1	39	dep	function	215:222	arg1	the					200:202	the	200:202	the	200:202	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	1	40	theme	known	164:168	arg1	relevance					178:186	the known disease relevance	160:186	the known disease relevance of glycans	160:197	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	1	41	theme	glycosyltransferases	266:285	arg1	function					215:222	biological function	204:222	biological function	204:222	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	1	41	theme	glycosyltransferases	266:285	arg1	specificities					238:250	substrate specificities	228:250	substrate specificities	228:250	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36633947	5	42	dep	use	807:809	arg1	the					803:805	the	803:805	the	803:805	For complete details on the use and execution of this protocol, please refer to Schumann et al. (2020)1, Cioce et al. (2021)2, and Cioce et al. (2022)3.
36633947	1	43	theme	disease	170:176	arg1	relevance					178:186	the known disease relevance	160:186	the known disease relevance of glycans	160:197	Despite the known disease relevance of glycans, the biological function and substrate specificities of individual glycosyltransferases are often ill-defined.
36375784	2	0	theme	IMI	490:492	arg1	exposure					494:501	IMI exposure	490:501	IMI exposure	490:501	Melatonin (MT) provides effective protection against insecticide-induced toxicity, nevertheless, the toxic effects and whether MT attenuates intestinal injury caused by IMI exposure in the common carps remains poorly explored.
36375784	7	1	theme	IMI-induced	1178:1188	arg1	toxicity					1201:1208	IMI-induced intestinal toxicity	1178:1208	IMI-induced intestinal toxicity	1178:1208	MT addition dramatically attenuated IMI-induced intestinal toxicity by remitting PGN synthesis and thus resecuring tight junction permeability, thereby reducing intestinal injury.
36375784	8	2	theme	junctions	1416:1424	arg1	permeability					1394:1405	the increased permeability	1380:1405	the increased permeability of tight junctions induced by IMI/PGN	1380:1443	SB203580 was supplied as a P38MAPK inhibitor to alleviate the increased permeability of tight junctions induced by IMI/PGN.
36375784	6	3	from	increase	1049:1056	arg1	permeability					1087:1098	intestinal tight junction permeability	1061:1098	intestinal tight junction permeability	1061:1098	Subsequently, we thus further elucidated that IMI induced an increase in intestinal tight junction permeability by inducing PGN secretion in vitro model.
36375784	8	4	theme	tight	1410:1414	arg1	junctions					1416:1424	tight junctions	1410:1424	tight junctions induced by IMI/PGN	1410:1443	SB203580 was supplied as a P38MAPK inhibitor to alleviate the increased permeability of tight junctions induced by IMI/PGN.
36375784	9	5	theme	IMI-induced	1507:1517	arg1	injury					1530:1535	IMI-induced intestinal injury	1507:1535	IMI-induced intestinal injury	1507:1535	Therefore, these findings confirmed that MT protects against IMI-induced intestinal injury by negatively regulating PGN/P38MAPK pathway to antagonize the increased tight junction permeability.
36375784	0	6	theme	common	117:122	arg1	Cyprinuscarpio					130:143	Cyprinuscarpio	130:143	Cyprinuscarpio	130:143	Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinuscarpio).
36375784	0	6	theme	common	117:122	arg1	carp					124:127	the common carp	113:127	the common carp (Cyprinuscarpio)	113:144	Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinuscarpio).
36375784	1	7	theme	surface	256:262	arg1	worldwide					270:278	surface water worldwide	256:278	surface water worldwide	256:278	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	4	8	dep	composition	694:704	arg1	the					690:692	the	690:692	the	690:692	Therefore, we first demonstrated that IMI altered the composition and function of the intestinal microbiota, destroying the integrity of intestinal ultrastructure, increasing intestinal permeability.
36375784	3	9	theme	Previous	548:555	arg1	studies					557:563	Previous studies	548:563	Previous studies	548:563	Previous studies have reported adverse effects of IMI exposure on intestinal health status.
36375784	1	10	theme	water	264:268	arg1	worldwide					270:278	surface water worldwide	256:278	surface water worldwide	256:278	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	2	11	theme	common	510:515	arg1	carps					517:521	the common carps	506:521	the common carps	506:521	Melatonin (MT) provides effective protection against insecticide-induced toxicity, nevertheless, the toxic effects and whether MT attenuates intestinal injury caused by IMI exposure in the common carps remains poorly explored.
36375784	7	12	theme	MT	1142:1143	arg1	addition					1145:1152	MT addition	1142:1152	MT addition	1142:1152	MT addition dramatically attenuated IMI-induced intestinal toxicity by remitting PGN synthesis and thus resecuring tight junction permeability, thereby reducing intestinal injury.
36375784	4	13	theme	ultrastructure	788:801	arg1	integrity					764:772	the integrity	760:772	the integrity of intestinal ultrastructure	760:801	Therefore, we first demonstrated that IMI altered the composition and function of the intestinal microbiota, destroying the integrity of intestinal ultrastructure, increasing intestinal permeability.
36375784	6	14	theme	vitro	1129:1133	arg1	model					1135:1139	vitro model	1129:1139	vitro model	1129:1139	Subsequently, we thus further elucidated that IMI induced an increase in intestinal tight junction permeability by inducing PGN secretion in vitro model.
36375784	7	15	theme	junction	1263:1270	arg1	permeability					1272:1283	tight junction permeability	1257:1283	tight junction permeability	1257:1283	MT addition dramatically attenuated IMI-induced intestinal toxicity by remitting PGN synthesis and thus resecuring tight junction permeability, thereby reducing intestinal injury.
36375784	4	16	theme	intestinal	777:786	arg1	ultrastructure					788:801	intestinal ultrastructure	777:801	intestinal ultrastructure	777:801	Therefore, we first demonstrated that IMI altered the composition and function of the intestinal microbiota, destroying the integrity of intestinal ultrastructure, increasing intestinal permeability.
36375784	7	17	theme	tight	1257:1261	arg1	permeability					1272:1283	tight junction permeability	1257:1283	tight junction permeability	1257:1283	MT addition dramatically attenuated IMI-induced intestinal toxicity by remitting PGN synthesis and thus resecuring tight junction permeability, thereby reducing intestinal injury.
36375784	8	18	theme	increased	1384:1392	arg1	permeability					1394:1405	the increased permeability	1380:1405	the increased permeability of tight junctions induced by IMI/PGN	1380:1443	SB203580 was supplied as a P38MAPK inhibitor to alleviate the increased permeability of tight junctions induced by IMI/PGN.
36375784	7	19	theme	intestinal	1303:1312	arg1	injury					1314:1319	intestinal injury	1303:1319	intestinal injury	1303:1319	MT addition dramatically attenuated IMI-induced intestinal toxicity by remitting PGN synthesis and thus resecuring tight junction permeability, thereby reducing intestinal injury.
36375784	2	20	theme	insecticide-induced	374:392	arg1	toxicity					394:401	insecticide-induced toxicity	374:401	insecticide-induced toxicity	374:401	Melatonin (MT) provides effective protection against insecticide-induced toxicity, nevertheless, the toxic effects and whether MT attenuates intestinal injury caused by IMI exposure in the common carps remains poorly explored.
36375784	3	21	theme	adverse	579:585	arg1	effects					587:593	adverse effects	579:593	adverse effects of IMI exposure on intestinal health status	579:637	Previous studies have reported adverse effects of IMI exposure on intestinal health status.
36375784	3	22	from	effects	587:593	arg1	status					632:637	intestinal health status	614:637	intestinal health status	614:637	Previous studies have reported adverse effects of IMI exposure on intestinal health status.
36375784	6	23	theme	PGN	1112:1114	arg1	secretion					1116:1124	PGN secretion	1112:1124	PGN secretion	1112:1124	Subsequently, we thus further elucidated that IMI induced an increase in intestinal tight junction permeability by inducing PGN secretion in vitro model.
36375784	0	24	theme	intestinal	43:52	arg1	injury					54:59	imidacloprid-induced intestinal injury	22:59	imidacloprid-induced intestinal injury	22:59	Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinuscarpio).
36375784	4	25	theme	microbiota	737:746	arg1	composition					694:704	composition	694:704	composition	694:704	Therefore, we first demonstrated that IMI altered the composition and function of the intestinal microbiota, destroying the integrity of intestinal ultrastructure, increasing intestinal permeability.
36375784	4	25	theme	microbiota	737:746	arg1	function					710:717	function	710:717	function	710:717	Therefore, we first demonstrated that IMI altered the composition and function of the intestinal microbiota, destroying the integrity of intestinal ultrastructure, increasing intestinal permeability.
36375784	2	26	theme	toxic	422:426	arg1	effects					428:434	the toxic effects	418:434	the toxic effects	418:434	Melatonin (MT) provides effective protection against insecticide-induced toxicity, nevertheless, the toxic effects and whether MT attenuates intestinal injury caused by IMI exposure in the common carps remains poorly explored.
36375784	0	27	theme	imidacloprid-induced	22:41	arg1	injury					54:59	imidacloprid-induced intestinal injury	22:59	imidacloprid-induced intestinal injury	22:59	Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinuscarpio).
36375784	9	28	theme	junction	1616:1623	arg1	permeability					1625:1636	the increased tight junction permeability	1596:1636	the increased tight junction permeability	1596:1636	Therefore, these findings confirmed that MT protects against IMI-induced intestinal injury by negatively regulating PGN/P38MAPK pathway to antagonize the increased tight junction permeability.
36375784	1	29	theme	used	194:197	arg1	insecticides					213:224	the most frequently used neonicotinoid insecticides	174:224	the most frequently used neonicotinoid insecticides in agriculture	174:239	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	9	30	theme	tight	1610:1614	arg1	junction					1616:1623	the increased tight junction	1596:1623	the increased tight junction permeability	1596:1636	Therefore, these findings confirmed that MT protects against IMI-induced intestinal injury by negatively regulating PGN/P38MAPK pathway to antagonize the increased tight junction permeability.
36375784	5	31	theme	ELISA	878:882	arg1	kits					884:887	ELISA kits	878:887	ELISA kits	878:887	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	3	32	theme	IMI	598:600	arg1	exposure					602:609	IMI exposure	598:609	IMI exposure	598:609	Previous studies have reported adverse effects of IMI exposure on intestinal health status.
36375784	9	33	theme	PGN/P38MAPK	1562:1572	arg1	pathway					1574:1580	PGN/P38MAPK pathway	1562:1580	PGN/P38MAPK pathway	1562:1580	Therefore, these findings confirmed that MT protects against IMI-induced intestinal injury by negatively regulating PGN/P38MAPK pathway to antagonize the increased tight junction permeability.
36375784	1	34	theme	neonicotinoid	199:211	arg1	insecticides					213:224	the most frequently used neonicotinoid insecticides	174:224	the most frequently used neonicotinoid insecticides in agriculture	174:239	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	7	35	theme	PGN	1223:1225	arg1	synthesis					1227:1235	PGN synthesis	1223:1235	PGN synthesis	1223:1235	MT addition dramatically attenuated IMI-induced intestinal toxicity by remitting PGN synthesis and thus resecuring tight junction permeability, thereby reducing intestinal injury.
36375784	3	36	theme	exposure	602:609	arg1	effects					587:593	adverse effects	579:593	adverse effects of IMI exposure on intestinal health status	579:637	Previous studies have reported adverse effects of IMI exposure on intestinal health status.
36375784	1	37	theme	insecticides	213:224	arg1	one					167:169	one	167:169	one	167:169	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	1	37	theme	insecticides	213:224	arg1	Imidacloprid					147:158	Imidacloprid	147:158	Imidacloprid (IMI)	147:164	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	1	37	theme	insecticides	213:224	arg1	insecticides					213:224	the most frequently used neonicotinoid insecticides	174:224	the most frequently used neonicotinoid insecticides in agriculture	174:239	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	2	38	theme	intestinal	462:471	arg1	injury					473:478	intestinal injury	462:478	intestinal injury caused by IMI exposure	462:501	Melatonin (MT) provides effective protection against insecticide-induced toxicity, nevertheless, the toxic effects and whether MT attenuates intestinal injury caused by IMI exposure in the common carps remains poorly explored.
36375784	5	39	dep	results	889:895	arg1	hypothesized					897:908	hypothesized	897:908	results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite	889:985	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	1	40	from	insecticides	213:224	arg1	agriculture					229:239	agriculture	229:239	agriculture	229:239	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	9	41	theme	increased	1600:1608	arg1	junction					1616:1623	the increased tight junction	1596:1623	the increased tight junction permeability	1596:1636	Therefore, these findings confirmed that MT protects against IMI-induced intestinal injury by negatively regulating PGN/P38MAPK pathway to antagonize the increased tight junction permeability.
36375784	1	42	theme	aquatic	302:308	arg1	organisms					310:318	aquatic organisms	302:318	aquatic organisms	302:318	Imidacloprid (IMI), one of the most frequently used neonicotinoid insecticides in agriculture, is resided in surface water worldwide and poses a threat to aquatic organisms.
36375784	7	43	theme	intestinal	1190:1199	arg1	toxicity					1201:1208	IMI-induced intestinal toxicity	1178:1208	IMI-induced intestinal toxicity	1178:1208	MT addition dramatically attenuated IMI-induced intestinal toxicity by remitting PGN synthesis and thus resecuring tight junction permeability, thereby reducing intestinal injury.
36375784	5	44	theme	IMI-triggered	941:953	arg1	metabolite					976:985	an IMI-triggered intestinal microbial metabolite	938:985	an IMI-triggered intestinal microbial metabolite	938:985	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	5	44	theme	IMI-triggered	941:953	arg1	peptidoglycan					915:927	peptidoglycan	915:927	peptidoglycan (PGN)	915:933	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	4	45	theme	intestinal	815:824	arg1	permeability					826:837	intestinal permeability	815:837	intestinal permeability	815:837	Therefore, we first demonstrated that IMI altered the composition and function of the intestinal microbiota, destroying the integrity of intestinal ultrastructure, increasing intestinal permeability.
36375784	3	46	theme	health	625:630	arg1	status					632:637	intestinal health status	614:637	intestinal health status	614:637	Previous studies have reported adverse effects of IMI exposure on intestinal health status.
36375784	6	47	theme	intestinal	1061:1070	arg1	permeability					1087:1098	intestinal tight junction permeability	1061:1098	intestinal tight junction permeability	1061:1098	Subsequently, we thus further elucidated that IMI induced an increase in intestinal tight junction permeability by inducing PGN secretion in vitro model.
36375784	4	48	theme	intestinal	726:735	arg1	microbiota					737:746	the intestinal microbiota	722:746	the intestinal microbiota	722:746	Therefore, we first demonstrated that IMI altered the composition and function of the intestinal microbiota, destroying the integrity of intestinal ultrastructure, increasing intestinal permeability.
36375784	6	49	theme	tight	1072:1076	arg1	permeability					1087:1098	intestinal tight junction permeability	1061:1098	intestinal tight junction permeability	1061:1098	Subsequently, we thus further elucidated that IMI induced an increase in intestinal tight junction permeability by inducing PGN secretion in vitro model.
36375784	5	50	theme	metagenomic	851:861	arg1	sequencing					863:872	metagenomic sequencing	851:872	metagenomic sequencing	851:872	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	5	51	theme	microbial	966:974	arg1	metabolite					976:985	an IMI-triggered intestinal microbial metabolite	938:985	an IMI-triggered intestinal microbial metabolite	938:985	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	5	51	theme	microbial	966:974	arg1	peptidoglycan					915:927	peptidoglycan	915:927	peptidoglycan (PGN)	915:933	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	5	52	theme	intestinal	955:964	arg1	metabolite					976:985	an IMI-triggered intestinal microbial metabolite	938:985	an IMI-triggered intestinal microbial metabolite	938:985	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	5	52	theme	intestinal	955:964	arg1	peptidoglycan					915:927	peptidoglycan	915:927	peptidoglycan (PGN)	915:933	Meanwhile, metagenomic sequencing and ELISA kits results hypothesized that peptidoglycan (PGN) is an IMI-triggered intestinal microbial metabolite.
36375784	0	53	theme	PGN/P38MAPK	90:100	arg1	pathway					102:108	the PGN/P38MAPK pathway	86:108	the PGN/P38MAPK pathway	86:108	Melatonin ameliorates imidacloprid-induced intestinal injury by negatively regulating the PGN/P38MAPK pathway in the common carp (Cyprinuscarpio).
36375784	6	54	theme	junction	1078:1085	arg1	permeability					1087:1098	intestinal tight junction permeability	1061:1098	intestinal tight junction permeability	1061:1098	Subsequently, we thus further elucidated that IMI induced an increase in intestinal tight junction permeability by inducing PGN secretion in vitro model.
36375784	8	55	theme	P38MAPK	1349:1355	arg1	SB203580					1322:1329	SB203580	1322:1329	SB203580	1322:1329	SB203580 was supplied as a P38MAPK inhibitor to alleviate the increased permeability of tight junctions induced by IMI/PGN.
36375784	8	55	theme	P38MAPK	1349:1355	arg1	inhibitor					1357:1365	a P38MAPK inhibitor	1347:1365	a P38MAPK inhibitor to alleviate the increased permeability of tight junctions induced by IMI/PGN	1347:1443	SB203580 was supplied as a P38MAPK inhibitor to alleviate the increased permeability of tight junctions induced by IMI/PGN.
36375784	9	56	theme	intestinal	1519:1528	arg1	injury					1530:1535	IMI-induced intestinal injury	1507:1535	IMI-induced intestinal injury	1507:1535	Therefore, these findings confirmed that MT protects against IMI-induced intestinal injury by negatively regulating PGN/P38MAPK pathway to antagonize the increased tight junction permeability.
36375784	3	57	theme	intestinal	614:623	arg1	status					632:637	intestinal health status	614:637	intestinal health status	614:637	Previous studies have reported adverse effects of IMI exposure on intestinal health status.
36375784	2	58	theme	effective	345:353	arg1	protection					355:364	effective protection	345:364	effective protection against insecticide-induced toxicity	345:401	Melatonin (MT) provides effective protection against insecticide-induced toxicity, nevertheless, the toxic effects and whether MT attenuates intestinal injury caused by IMI exposure in the common carps remains poorly explored.
37346113	6	0	theme	original	901:908	arg1	sites					921:925	the original collection sites	897:925	the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms	897:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	6	0	theme	original	901:908	arg1	genotypes					945:953	592 Miscanthus genotypes	930:953	592 Miscanthus genotypes	930:953	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	10	1	theme	cell	1965:1968	arg1	recalcitrance					1975:1987	cell wall recalcitrance	1965:1987	cell wall recalcitrance	1965:1987	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	7	2	theme	genus	1132:1136	arg1	Miscanthus					1138:1147	the genus Miscanthus	1128:1147	the genus Miscanthus	1128:1147	Our results show that species of the genus Miscanthus grow naturally across a range of soil and climate conditions.
37346113	3	3	theme	genetic	495:501	arg1	variation					503:511	genetic variation	495:511	genetic variation for these characteristics	495:537	Local adaptation has likely shaped genetic variation for these characteristics and is therefore important to quantify.
37346113	4	4	theme	biomass	585:591	arg1	ease					634:637	the ease	630:637	the ease of conversion of cell wall polysaccharides to monomeric sugars	630:700	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	4	4	theme	biomass	585:591	arg1	parameter					601:609	A key biomass quality parameter	579:609	A key biomass quality parameter for biorefining	579:625	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	6	5	theme	climatic	874:881	arg1	conditions					883:892	climatic conditions	874:892	climatic conditions	874:892	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	9	6	theme	breeding	1702:1709	arg1	programmes					1711:1720	Miscanthus breeding programmes	1691:1720	Miscanthus breeding programmes	1691:1720	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	10	7	theme	sugar	1992:1996	arg1	release					1998:2004	sugar release	1992:2004	sugar release	1992:2004	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	9	8	used	used	1671:1674	arg2	variation					1599:1607	both inter- and intra- specific variation	1567:1607	both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance	1567:1662	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	8	9	theme	representative	1246:1259	arg1	genotypes					1261:1269	49 representative genotypes	1243:1269	49 representative genotypes	1243:1269	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	0	10	theme	geographical	116:127	arg1	origin					129:134	geographical origin	116:134	geographical origin	116:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	2	11	theme	varieties	279:287	arg1	creation					263:270	the creation	259:270	the creation of new varieties	259:287	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	5	12	theme	genetic	823:829	arg1	backgrounds					831:841	limited genetic backgrounds	815:841	limited genetic backgrounds	815:841	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	6	13	theme	genotypes	945:953	arg1	sites					921:925	the original collection sites	897:925	the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms	897:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	6	13	theme	genotypes	945:953	arg1	genotypes					945:953	592 Miscanthus genotypes	930:953	592 Miscanthus genotypes	930:953	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	10	14	dep	reflecting	1859:1868	arg1	addition					1847:1854	addition	1847:1854	addition	1847:1854	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	2	15	theme	Miscanthus	228:237	arg1	improvement					213:223	The improvement	209:223	The improvement of Miscanthus as a crop	209:247	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	5	16	from	backgrounds	831:841	arg1	accessions					799:808	accessions	799:808	accessions from limited genetic backgrounds	799:841	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	8	17	theme	detailed	1222:1229	arg1	analysis					1231:1238	a detailed analysis	1220:1238	a detailed analysis of 49 representative genotypes	1220:1269	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	6	18	theme	592	930:932	arg1	genotypes					945:953	592 Miscanthus genotypes	930:953	592 Miscanthus genotypes	930:953	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	0	19	from	genotypes	58:66	arg1	groups					105:110	different genetic groups	87:110	different genetic groups	87:110	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	0	19	from	genotypes	58:66	arg1	origin					129:134	geographical origin	116:134	geographical origin	116:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	8	20	theme	minor	1292:1296	arg1	differences					1298:1308	minor differences	1292:1308	minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups	1292:1421	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	0	21	from	groups	105:110	arg1	Miscanthus					71:80	Miscanthus	71:80	Miscanthus from different genetic groups and geographical origin	71:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	0	21	from	groups	105:110	arg1	genotypes					58:66	genotypes	58:66	genotypes of Miscanthus from different genetic groups and geographical origin	58:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	9	22	from	variation	1599:1607	arg1	recalcitrance					1650:1662	biomass recalcitrance	1642:1662	biomass recalcitrance	1642:1662	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	9	22	from	variation	1599:1607	arg1	wall					1617:1620	cell wall characteristics and biomass recalcitrance	1612:1662	wall	1617:1620	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	6	23	theme	sites	921:925	arg1	soil					865:868	soil	865:868	soil	865:868	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	6	23	theme	sites	921:925	arg1	conditions					883:892	climatic conditions	874:892	climatic conditions	874:892	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	9	24	theme	biomass	1776:1782	arg1	yield					1784:1788	biomass yield	1776:1788	biomass yield when quantifying overall conversion efficiency	1776:1835	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	8	25	theme	lower	1482:1486	arg1	recalcitrance					1488:1500	lower recalcitrance	1482:1500	lower recalcitrance	1482:1500	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	10	26	theme	breeding	2079:2086	arg1	programmes					2088:2097	breeding programmes	2079:2097	breeding programmes targeted at improving the Miscanthus biomass crop	2079:2147	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	6	27	theme	discriminant	1006:1017	arg1	analysis					1019:1026	discriminant analysis	1006:1026	discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms	1006:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	8	28	theme	wall	1318:1321	arg1	characteristics					1323:1337	cell wall characteristics	1313:1337	cell wall characteristics	1313:1337	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	5	29	theme	related	742:748	arg1	traits					750:755	cell wall related traits	732:755	cell wall related traits in Miscanthus	732:769	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	9	30	theme	overall	1807:1813	arg1	efficiency					1826:1835	overall conversion efficiency	1807:1835	overall conversion efficiency	1807:1835	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	0	31	theme	Miscanthus	71:80	arg1	genotypes					58:66	genotypes	58:66	genotypes of Miscanthus from different genetic groups and geographical origin	58:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	6	32	theme	genetic	982:988	arg1	groups					990:995	eight distinct genetic groups	967:995	eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms	967:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	8	33	theme	genetic	1524:1530	arg1	groups					1532:1537	the other genetic groups	1514:1537	the other genetic groups	1514:1537	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	8	34	theme	genetic	1357:1363	arg1	groups					1365:1370	different genetic groups	1347:1370	different genetic groups	1347:1370	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	8	34	theme	genetic	1357:1363	arg1	variation					1399:1407	genetic variation	1391:1407	genetic variation within groups	1391:1421	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	0	35	theme	different	87:95	arg1	groups					105:110	different genetic groups	87:110	different genetic groups	87:110	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	5	36	theme	cell	732:735	arg1	traits					750:755	cell wall related traits	732:755	cell wall related traits in Miscanthus	732:769	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	6	37	theme	polymorphisms	1080:1092	arg1	components					1041:1050	principal components	1031:1050	principal components of 25,014 single-nucleotide polymorphisms	1031:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	2	38	theme	quality	436:442	arg1	terms					405:409	terms	405:409	terms of resilience, yield and quality of the biomass	405:457	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	7	39	theme	soil	1182:1185	arg1	conditions					1199:1208	soil and climate conditions	1182:1208	soil and climate conditions	1182:1208	Our results show that species of the genus Miscanthus grow naturally across a range of soil and climate conditions.
37346113	9	40	theme	specific	1590:1597	arg1	variation					1599:1607	both inter- and intra- specific variation	1567:1607	both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance	1567:1662	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	2	41	theme	yield	426:430	arg1	terms					405:409	terms	405:409	terms of resilience, yield and quality of the biomass	405:457	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	8	42	theme	genetic	1391:1397	arg1	variation					1399:1407	genetic variation	1391:1407	genetic variation within groups	1391:1421	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	2	43	theme	resilience	414:423	arg1	terms					405:409	terms	405:409	terms of resilience, yield and quality of the biomass	405:457	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	0	44	theme	cell	15:18	arg1	wall					20:23	cell wall recalcitrance and composition	15:53	cell wall recalcitrance and composition	15:53	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	6	45	theme	components	1041:1050	arg1	analysis					1019:1026	discriminant analysis	1006:1026	discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms	1006:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	10	46	theme	compositional	1913:1925	arg1	features					1952:1959	compositional and structural cell wall features	1913:1959	compositional and structural cell wall features	1913:1959	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	10	47	theme	interactions	1892:1903	arg1	complexity					1874:1883	the complexity	1870:1883	the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance	1870:1987	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	2	48	from	characteristics	386:400	arg1	terms					405:409	terms	405:409	terms of resilience, yield and quality of the biomass	405:457	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	10	49	theme	wall	1947:1950	arg1	features					1952:1959	compositional and structural cell wall features	1913:1959	compositional and structural cell wall features	1913:1959	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	2	50	theme	germplasm	318:326	arg1	hybridization					301:313	the hybridization	297:313	the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass	297:457	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	4	51	theme	polysaccharides	666:680	arg1	conversion					642:651	conversion	642:651	conversion of cell wall polysaccharides to monomeric sugars	642:700	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	10	52	theme	Miscanthus	2125:2134	arg1	crop					2144:2147	the Miscanthus biomass crop	2121:2147	the Miscanthus biomass crop	2121:2147	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	4	53	theme	quality	593:599	arg1	ease					634:637	the ease	630:637	the ease of conversion of cell wall polysaccharides to monomeric sugars	630:700	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	4	53	theme	quality	593:599	arg1	parameter					601:609	A key biomass quality parameter	579:609	A key biomass quality parameter for biorefining	579:625	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	10	54	theme	structural	1931:1940	arg1	features					1952:1959	compositional and structural cell wall features	1913:1959	compositional and structural cell wall features	1913:1959	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	2	55	theme	genetic	355:361	arg1	variation					363:371	genetic variation	355:371	genetic variation	355:371	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	6	56	theme	collection	910:919	arg1	sites					921:925	the original collection sites	897:925	the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms	897:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	6	56	theme	collection	910:919	arg1	genotypes					945:953	592 Miscanthus genotypes	930:953	592 Miscanthus genotypes	930:953	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	10	57	theme	wall	1970:1973	arg1	recalcitrance					1975:1987	cell wall recalcitrance	1965:1987	cell wall recalcitrance	1965:1987	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	2	58	theme	new	275:277	arg1	varieties					279:287	new varieties	275:287	new varieties	275:287	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	9	59	theme	Miscanthus	1691:1700	arg1	programmes					1711:1720	Miscanthus breeding programmes	1691:1720	Miscanthus breeding programmes	1691:1720	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	4	60	theme	key	581:583	arg1	ease					634:637	the ease	630:637	the ease of conversion of cell wall polysaccharides to monomeric sugars	630:700	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	4	60	theme	key	581:583	arg1	parameter					601:609	A key biomass quality parameter	579:609	A key biomass quality parameter for biorefining	579:625	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	4	61	theme	wall	661:664	arg1	polysaccharides					666:680	cell wall polysaccharides	656:680	cell wall polysaccharides	656:680	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	4	62	theme	conversion	642:651	arg1	ease					634:637	the ease	630:637	the ease of conversion of cell wall polysaccharides to monomeric sugars	630:700	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	4	62	theme	conversion	642:651	arg1	parameter					601:609	A key biomass quality parameter	579:609	A key biomass quality parameter for biorefining	579:625	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	5	63	theme	in	757:758	arg1	traits					750:755	cell wall related traits	732:755	cell wall related traits in Miscanthus	732:769	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	4	64	theme	cell	656:659	arg1	polysaccharides					666:680	cell wall polysaccharides	656:680	cell wall polysaccharides	656:680	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	5	65	theme	limited	815:821	arg1	backgrounds					831:841	limited genetic backgrounds	815:841	limited genetic backgrounds	815:841	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	5	66	dep	in	757:758	arg1	Miscanthus					760:769	Miscanthus	760:769	Miscanthus	760:769	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	8	67	theme	genotypes	1261:1269	arg1	analysis					1231:1238	a detailed analysis	1220:1238	a detailed analysis of 49 representative genotypes	1220:1269	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	9	68	dep	wall	1617:1620	arg1	characteristics					1622:1636	characteristics	1622:1636	characteristics	1622:1636	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	6	69	theme	Miscanthus	934:943	arg1	genotypes					945:953	592 Miscanthus genotypes	930:953	592 Miscanthus genotypes	930:953	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	7	70	theme	conditions	1199:1208	arg1	range					1173:1177	a range	1171:1177	a range of soil and climate conditions	1171:1208	Our results show that species of the genus Miscanthus grow naturally across a range of soil and climate conditions.
37346113	0	71	from	Variability	0:10	arg1	genotypes					58:66	genotypes	58:66	genotypes of Miscanthus from different genetic groups and geographical origin	58:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	0	72	dep	wall	20:23	arg1	composition					43:53	composition	43:53	composition	43:53	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	0	72	dep	wall	20:23	arg1	recalcitrance					25:37	recalcitrance	25:37	recalcitrance	25:37	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	8	73	from	differences	1298:1308	arg1	characteristics					1323:1337	cell wall characteristics	1313:1337	cell wall characteristics	1313:1337	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	3	74	theme	Local	460:464	arg1	adaptation					466:475	Local adaptation	460:475	Local adaptation	460:475	Local adaptation has likely shaped genetic variation for these characteristics and is therefore important to quantify.
37346113	5	75	theme	wall	737:740	arg1	traits					750:755	cell wall related traits	732:755	cell wall related traits in Miscanthus	732:769	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	8	76	theme	other	1518:1522	arg1	groups					1532:1537	the other genetic groups	1514:1537	the other genetic groups	1514:1537	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	8	77	theme	cell	1313:1316	arg1	characteristics					1323:1337	cell wall characteristics	1313:1337	cell wall characteristics	1313:1337	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	5	78	theme	traits	750:755	arg1	variability					717:727	the variability	713:727	the variability of cell wall related traits in Miscanthus	713:769	Thus far, the variability of cell wall related traits in Miscanthus has mostly been explored in accessions from limited genetic backgrounds.
37346113	1	79	theme	promising	153:161	arg1	Miscanthus					137:146	Miscanthus	137:146	Miscanthus	137:146	Miscanthus is a promising crop for bioenergy and biorefining in Europe.
37346113	1	79	theme	promising	153:161	arg1	crop					163:166	a promising crop	151:166	a promising crop for bioenergy and biorefining in Europe	151:206	Miscanthus is a promising crop for bioenergy and biorefining in Europe.
37346113	9	80	theme	conversion	1815:1824	arg1	efficiency					1826:1835	overall conversion efficiency	1807:1835	overall conversion efficiency	1807:1835	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	4	81	theme	monomeric	685:693	arg1	sugars					695:700	monomeric sugars	685:700	monomeric sugars	685:700	A key biomass quality parameter for biorefining is the ease of conversion of cell wall polysaccharides to monomeric sugars.
37346113	0	82	theme	genetic	97:103	arg1	groups					105:110	different genetic groups	87:110	different genetic groups	87:110	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	1	83	from	crop	163:166	arg1	Europe					201:206	Europe	201:206	Europe	201:206	Miscanthus is a promising crop for bioenergy and biorefining in Europe.
37346113	6	84	theme	distinct	973:980	arg1	groups					990:995	eight distinct genetic groups	967:995	eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms	967:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	8	85	theme	different	1347:1355	arg1	groups					1365:1370	different genetic groups	1347:1370	different genetic groups	1347:1370	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	8	85	theme	different	1347:1355	arg1	variation					1399:1407	genetic variation	1391:1407	genetic variation within groups	1391:1421	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	9	86	theme	inter-	1572:1577	arg1	variation					1599:1607	both inter- and intra- specific variation	1567:1607	both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance	1567:1662	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	6	87	dep	soil	865:868	arg1	the					861:863	the	861:863	the	861:863	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	2	88	theme	suitable	377:384	arg1	characteristics					386:400	suitable characteristics	377:400	suitable characteristics	377:400	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	9	89	theme	intra-	1583:1588	arg1	variation					1599:1607	both inter- and intra- specific variation	1567:1607	both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance	1567:1662	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	6	90	theme	single-nucleotide	1062:1078	arg1	polymorphisms					1080:1092	25,014 single-nucleotide polymorphisms	1055:1092	25,014 single-nucleotide polymorphisms	1055:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	8	91	theme	high	1376:1379	arg1	levels					1381:1386	high levels	1376:1386	high levels of genetic variation within groups	1376:1421	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	0	92	theme	wall	20:23	arg1	Variability					0:10	Variability	0:10	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.	0:135	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	9	93	theme	cell	1612:1615	arg1	wall					1617:1620	cell wall characteristics and biomass recalcitrance	1612:1662	wall	1617:1620	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	6	94	theme	principal	1031:1039	arg1	components					1041:1050	principal components	1031:1050	principal components of 25,014 single-nucleotide polymorphisms	1031:1092	Here we analysed the soil and climatic conditions of the original collection sites of 592 Miscanthus genotypes, which form eight distinct genetic groups based on discriminant analysis of principal components of 25,014 single-nucleotide polymorphisms.
37346113	2	95	theme	biomass	451:457	arg1	quality					436:442	quality	436:442	quality	436:442	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	2	95	theme	biomass	451:457	arg1	yield					426:430	yield	426:430	yield	426:430	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	2	95	theme	biomass	451:457	arg1	resilience					414:423	resilience	414:423	resilience	414:423	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	8	96	theme	variation	1399:1407	arg1	groups					1365:1370	different genetic groups	1347:1370	different genetic groups	1347:1370	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	8	96	theme	variation	1399:1407	arg1	levels					1381:1386	high levels	1376:1386	high levels of genetic variation within groups	1376:1421	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	8	96	theme	variation	1399:1407	arg1	variation					1399:1407	genetic variation	1391:1407	genetic variation within groups	1391:1421	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
37346113	7	97	theme	climate	1191:1197	arg1	conditions					1199:1208	soil and climate conditions	1182:1208	soil and climate conditions	1182:1208	Our results show that species of the genus Miscanthus grow naturally across a range of soil and climate conditions.
37346113	7	98	theme	Miscanthus	1138:1147	arg1	species					1117:1123	species	1117:1123	species of the genus Miscanthus	1117:1147	Our results show that species of the genus Miscanthus grow naturally across a range of soil and climate conditions.
37346113	2	99	from	variation	363:371	arg1	terms					405:409	terms	405:409	terms of resilience, yield and quality of the biomass	405:457	The improvement of Miscanthus as a crop relies on the creation of new varieties through the hybridization of germplasm collected in the wild with genetic variation and suitable characteristics in terms of resilience, yield and quality of the biomass.
37346113	9	100	theme	biomass	1642:1648	arg1	recalcitrance					1650:1662	biomass recalcitrance	1642:1662	biomass recalcitrance	1642:1662	The results emphasize that both inter- and intra- specific variation in cell wall characteristics and biomass recalcitrance can be used effectively in Miscanthus breeding programmes, while also reinforcing the importance of considering biomass yield when quantifying overall conversion efficiency.
37346113	10	101	theme	biomass	2136:2142	arg1	crop					2144:2147	the Miscanthus biomass crop	2121:2147	the Miscanthus biomass crop	2121:2147	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	10	102	theme	cell	1942:1945	arg1	features					1952:1959	compositional and structural cell wall features	1913:1959	compositional and structural cell wall features	1913:1959	Thus, in addition to reflecting the complexity of the interactions between compositional and structural cell wall features and cell wall recalcitrance to sugar release, our results point to traits that could potentially require attention in breeding programmes targeted at improving the Miscanthus biomass crop.
37346113	0	103	from	origin	129:134	arg1	Miscanthus					71:80	Miscanthus	71:80	Miscanthus from different genetic groups and geographical origin	71:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	0	103	from	origin	129:134	arg1	genotypes					58:66	genotypes	58:66	genotypes of Miscanthus from different genetic groups and geographical origin	58:134	Variability of cell wall recalcitrance and composition in genotypes of Miscanthus from different genetic groups and geographical origin.
37346113	8	104	theme	investigated	1434:1445	arg1	species					1447:1453	less investigated species	1429:1453	less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups	1429:1537	Based on a detailed analysis of 49 representative genotypes, we report generally minor differences in cell wall characteristics between different genetic groups and high levels of genetic variation within groups, with less investigated species like M. floridulus showing lower recalcitrance compared to the other genetic groups.
35981631	6	0	from	samples	720:726	arg1	analysis					659:666	16s rRNA analysis	650:666	16s rRNA analysis	650:666	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	0	from	samples	720:726	arg1	analysis					745:752	metabolomics analysis	732:752	metabolomics analysis of blood samples	732:769	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	0	from	samples	720:726	arg1	measurement					672:682	measurement	672:682	measurement	672:682	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	14	1	from	microbes	1721:1728	arg1	BALF					1700:1703	BALF	1700:1703	BALF	1700:1703	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	12	2	theme	ABX + LPS	1514:1522	arg1	group					1524:1528	ABX + LPS group	1514:1528	ABX + LPS group	1514:1528	Untargeted metabolomics analysis identified several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group.
35981631	12	3	theme	several	1433:1439	arg1	metabolites					1448:1458	several plasma metabolites	1433:1458	several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group	1433:1528	Untargeted metabolomics analysis identified several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group.
35981631	5	4	theme	MAIN	495:498	arg1	METHODS					500:506	MAIN METHODS	495:506	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.	495:635	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	3	5	from	role	278:281	arg1	pathology					317:325	the pathology	313:325	the pathology of lung injury	313:340	An increasing evidence suggests the role of the gut-microbiota axis in the pathology of lung injury.
35981631	12	6	theme	responsible	1460:1470	arg1	metabolites					1448:1458	several plasma metabolites	1433:1458	several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group	1433:1528	Untargeted metabolomics analysis identified several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group.
35981631	4	7	theme	microbiome	402:411	arg1	depletion					413:421	antibiotic-induced microbiome depletion	383:421	antibiotic-induced microbiome depletion	383:421	This study aimed to investigate whether antibiotic-induced microbiome depletion could affect ALI in mice after lipopolysaccharide (LPS) administration.
35981631	8	8	dep	BALF	1009:1012	arg1	scores					1030:1035	scores	1030:1035	scores	1030:1035	Interestingly, an ABX significantly attenuated the LPS-induced increase in IL-6 in BALF and lung injury scores.
35981631	12	9	theme	Untargeted	1389:1398	arg1	analysis					1413:1420	Untargeted metabolomics analysis	1389:1420	Untargeted metabolomics analysis	1389:1420	Untargeted metabolomics analysis identified several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group.
35981631	11	10	theme	microbial	1350:1358	arg1	Clostriumtertium					1293:1308	Clostriumtertium	1293:1308	Clostriumtertium	1293:1308	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	11	10	theme	microbial	1350:1358	arg1	Bacteroidescaecimyris					1315:1335	Bacteroidescaecimyris	1315:1335	Bacteroidescaecimyris	1315:1335	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	11	10	theme	microbial	1350:1358	arg1	Enterococusfaecalis					1272:1290	Enterococusfaecalis	1272:1290	Enterococusfaecalis	1272:1290	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	11	10	theme	microbial	1350:1358	arg1	markers					1360:1366	potential microbial markers	1340:1366	potential microbial markers for ABX + LPS group	1340:1386	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	6	11	theme	samples	763:769	arg1	analysis					659:666	16s rRNA analysis	650:666	16s rRNA analysis	650:666	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	11	theme	samples	763:769	arg1	analysis					745:752	metabolomics analysis	732:752	metabolomics analysis of blood samples	732:769	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	11	theme	samples	763:769	arg1	measurement					672:682	measurement	672:682	measurement	672:682	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	5	12	from	ALI	552:554	arg1	mice					563:566	the mice	559:566	the mice	559:566	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	15	13	theme	LPS-induced	1847:1857	arg1	ALI					1859:1861	LPS-induced ALI	1847:1861	LPS-induced ALI	1847:1861	SIGNIFICANCE These data suggest that ABX-induced microbiome depletion could protect against LPS-induced ALI via the gut-microbiota-lung axis.
35981631	10	14	from	differences	1162:1172	arg1	α-					1181:1182	α-	1181:1182	α-	1181:1182	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	10	14	from	differences	1162:1172	arg1	β-diversity					1188:1198	β-diversity	1188:1198	β-diversity	1188:1198	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	2	15	theme	ALI	222:224	arg1	pathology					209:217	the pathology	205:217	the pathology of ALI	205:224	However, the precise mechanisms underlying the pathology of ALI remain elusive.
35981631	3	16	theme	gut-microbiota	290:303	arg1	axis					305:308	the gut-microbiota axis	286:308	the gut-microbiota axis	286:308	An increasing evidence suggests the role of the gut-microbiota axis in the pathology of lung injury.
35981631	4	17	theme	lipopolysaccharide	454:471	arg1	administration					479:492	lipopolysaccharide (LPS) administration	454:492	lipopolysaccharide (LPS) administration	454:492	This study aimed to investigate whether antibiotic-induced microbiome depletion could affect ALI in mice after lipopolysaccharide (LPS) administration.
35981631	4	18	from	ALI	436:438	arg1	mice					443:446	mice	443:446	mice	443:446	This study aimed to investigate whether antibiotic-induced microbiome depletion could affect ALI in mice after lipopolysaccharide (LPS) administration.
35981631	5	19	dep	METHODS	500:506	arg1	examined					627:634	examined	627:634	were examined	622:634	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	10	20	theme	water + LPS	1207:1217	arg1	group					1219:1223	the water + LPS group	1203:1223	the water + LPS group	1203:1223	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	14	21	theme	plasma	1734:1739	arg1	metabolites					1741:1751	plasma metabolites	1734:1751	plasma metabolites	1734:1751	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	14	21	theme	plasma	1734:1739	arg1	microbes					1721:1728	several gut microbes	1709:1728	several gut microbes (or plasma metabolites)	1709:1752	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	5	22	theme	cocktail	534:541	arg1	effects					512:518	The effects	508:518	The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg)	508:620	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	1	23	theme	acute	133:137	arg1	AIMS					98:101	AIMS Acute lung injury (ALI)	98:125	AIMS Acute lung injury (ALI)	98:125	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	1	23	theme	acute	133:137	arg1	disorder					152:159	an acute inflammatory disorder	130:159	an acute inflammatory disorder	130:159	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	14	24	theme	network	1640:1646	arg1	analysis					1648:1655	Integrative network analysis	1628:1655	Integrative network analysis	1628:1655	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	1	25	dep	AIMS	98:101	arg1	ALI					122:124	ALI	122:124	ALI	122:124	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	1	25	dep	AIMS	98:101	arg1	injury					114:119	Acute lung injury	103:119	AIMS Acute lung injury (ALI)	98:125	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	10	26	theme	group	1239:1243	arg1	α-					1181:1182	α-	1181:1182	α-	1181:1182	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	10	26	theme	group	1239:1243	arg1	β-diversity					1188:1198	β-diversity	1188:1198	β-diversity	1188:1198	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	5	27	theme	intratracheally	574:588	arg1	administration					590:603	intratracheally administration	574:603	intratracheally administration of LPS (5 mg/kg)	574:620	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	11	28	theme	LEfSe	1246:1250	arg1	analysis					1252:1259	LEfSe analysis	1246:1259	LEfSe analysis	1246:1259	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	15	29	theme	microbiome	1804:1813	arg1	depletion					1815:1823	ABX-induced microbiome depletion	1792:1823	ABX-induced microbiome depletion	1792:1823	SIGNIFICANCE These data suggest that ABX-induced microbiome depletion could protect against LPS-induced ALI via the gut-microbiota-lung axis.
35981631	14	30	theme	gut	1717:1719	arg1	metabolites					1741:1751	plasma metabolites	1734:1751	plasma metabolites	1734:1751	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	14	30	theme	gut	1717:1719	arg1	microbes					1721:1728	several gut microbes	1709:1728	several gut microbes (or plasma metabolites)	1709:1752	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	8	31	theme	lung	1018:1021	arg1	injury					1023:1028	lung injury	1018:1028	lung injury	1018:1028	Interestingly, an ABX significantly attenuated the LPS-induced increase in IL-6 in BALF and lung injury scores.
35981631	3	32	theme	lung	330:333	arg1	injury					335:340	lung injury	330:340	lung injury	330:340	An increasing evidence suggests the role of the gut-microbiota axis in the pathology of lung injury.
35981631	6	33	theme	short-chain	687:697	arg1	acids					705:709	short-chain fatty acids	687:709	short-chain fatty acids in feces samples	687:726	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	7	34	theme	mice	920:923	arg1	BALF					897:900	BALF	897:900	BALF	897:900	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	7	34	theme	mice	920:923	arg1	fluid					890:894	the bronchoalveolar lavage fluid	863:894	the bronchoalveolar lavage fluid (BALF) of water-treated mice	863:923	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	13	35	theme	relative	1567:1574	arg1	abundance					1576:1584	the relative abundance	1563:1584	the relative abundance of the microbiome and plasma metabolites	1563:1625	There were correlations between the relative abundance of the microbiome and plasma metabolites.
35981631	0	36	theme	Antibiotic-induced	0:17	arg1	depletion					30:38	Antibiotic-induced microbiome depletion	0:38	Antibiotic-induced microbiome depletion	0:38	Antibiotic-induced microbiome depletion improves LPS-induced acute lung injury via gut-lung axis.
35981631	7	37	theme	FINDINGS	791:798	arg1	LPS					800:802	KEY FINDINGS LPS	787:802	KEY FINDINGS LPS	787:802	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	1	38	theme	Acute	103:107	arg1	ALI					122:124	ALI	122:124	ALI	122:124	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	1	38	theme	Acute	103:107	arg1	injury					114:119	Acute lung injury	103:119	AIMS Acute lung injury (ALI)	98:125	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	6	39	theme	16s	650:652	arg1	analysis					659:666	16s rRNA analysis	650:666	16s rRNA analysis	650:666	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	0	40	theme	LPS-induced	49:59	arg1	injury					72:77	LPS-induced acute lung injury	49:77	LPS-induced acute lung injury	49:77	Antibiotic-induced microbiome depletion improves LPS-induced acute lung injury via gut-lung axis.
35981631	0	41	theme	lung	67:70	arg1	injury					72:77	LPS-induced acute lung injury	49:77	LPS-induced acute lung injury	49:77	Antibiotic-induced microbiome depletion improves LPS-induced acute lung injury via gut-lung axis.
35981631	5	42	from	effects	512:518	arg1	ALI					552:554	ALI	552:554	ALI in the mice after intratracheally administration of LPS (5 mg/kg)	552:620	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	6	43	from	analysis	745:752	arg1	samples					720:726	feces samples	714:726	feces samples	714:726	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	2	44	theme	precise	175:181	arg1	mechanisms					183:192	the precise mechanisms	171:192	the precise mechanisms underlying the pathology of ALI	171:224	However, the precise mechanisms underlying the pathology of ALI remain elusive.
35981631	6	45	theme	feces	714:718	arg1	samples					720:726	feces samples	714:726	feces samples	714:726	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	10	46	theme	significant	1150:1160	arg1	differences					1162:1172	significant differences	1150:1172	significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group	1150:1243	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	6	47	from	acids	705:709	arg1	samples					720:726	feces samples	714:726	feces samples	714:726	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	48	theme	acids	705:709	arg1	analysis					659:666	16s rRNA analysis	650:666	16s rRNA analysis	650:666	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	48	theme	acids	705:709	arg1	analysis					745:752	metabolomics analysis	732:752	metabolomics analysis of blood samples	732:769	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	48	theme	acids	705:709	arg1	measurement					672:682	measurement	672:682	measurement	672:682	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	7	49	theme	lavage	883:888	arg1	BALF					897:900	BALF	897:900	BALF	897:900	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	7	49	theme	lavage	883:888	arg1	fluid					890:894	the bronchoalveolar lavage fluid	863:894	the bronchoalveolar lavage fluid (BALF) of water-treated mice	863:923	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	4	50	theme	antibiotic-induced	383:400	arg1	depletion					413:421	antibiotic-induced microbiome depletion	383:421	antibiotic-induced microbiome depletion	383:421	This study aimed to investigate whether antibiotic-induced microbiome depletion could affect ALI in mice after lipopolysaccharide (LPS) administration.
35981631	8	51	from	increase	989:996	arg1	BALF					1009:1012	BALF	1009:1012	BALF	1009:1012	Interestingly, an ABX significantly attenuated the LPS-induced increase in IL-6 in BALF and lung injury scores.
35981631	8	51	from	increase	989:996	arg1	injury					1023:1028	lung injury	1018:1028	lung injury	1018:1028	Interestingly, an ABX significantly attenuated the LPS-induced increase in IL-6 in BALF and lung injury scores.
35981631	8	51	from	increase	989:996	arg1	IL-6					1001:1004	IL-6	1001:1004	IL-6	1001:1004	Interestingly, an ABX significantly attenuated the LPS-induced increase in IL-6 in BALF and lung injury scores.
35981631	15	52	dep	SIGNIFICANCE	1755:1766	arg1	suggest					1779:1785	suggest	1779:1785	suggest that ABX-induced microbiome depletion could protect against LPS-induced ALI via the gut-microbiota-lung axis	1779:1894	SIGNIFICANCE These data suggest that ABX-induced microbiome depletion could protect against LPS-induced ALI via the gut-microbiota-lung axis.
35981631	8	53	theme	LPS-induced	977:987	arg1	increase					989:996	the LPS-induced increase	973:996	the LPS-induced increase in IL-6 in BALF and lung injury scores	973:1035	Interestingly, an ABX significantly attenuated the LPS-induced increase in IL-6 in BALF and lung injury scores.
35981631	6	54	from	measurement	672:682	arg1	samples					720:726	feces samples	714:726	feces samples	714:726	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	12	55	theme	water + LPS	1491:1501	arg1	group					1503:1507	water + LPS group	1491:1507	water + LPS group	1491:1507	Untargeted metabolomics analysis identified several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group.
35981631	3	56	theme	increasing	245:254	arg1	evidence					256:263	An increasing evidence	242:263	An increasing evidence	242:263	An increasing evidence suggests the role of the gut-microbiota axis in the pathology of lung injury.
35981631	12	57	theme	plasma	1441:1446	arg1	metabolites					1448:1458	several plasma metabolites	1433:1458	several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group	1433:1528	Untargeted metabolomics analysis identified several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group.
35981631	11	58	theme	potential	1340:1348	arg1	Clostriumtertium					1293:1308	Clostriumtertium	1293:1308	Clostriumtertium	1293:1308	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	11	58	theme	potential	1340:1348	arg1	Bacteroidescaecimyris					1315:1335	Bacteroidescaecimyris	1315:1335	Bacteroidescaecimyris	1315:1335	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	11	58	theme	potential	1340:1348	arg1	Enterococusfaecalis					1272:1290	Enterococusfaecalis	1272:1290	Enterococusfaecalis	1272:1290	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	11	58	theme	potential	1340:1348	arg1	markers					1360:1366	potential microbial markers	1340:1366	potential microbial markers for ABX + LPS group	1340:1386	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	6	59	theme	blood	757:761	arg1	samples					763:769	blood samples	757:769	blood samples	757:769	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	7	60	theme	interleukin-6	832:844	arg1	levels					853:858	the interleukin-6 (IL-6) levels	828:858	the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice	828:923	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	12	61	theme	metabolomics	1400:1411	arg1	analysis					1413:1420	Untargeted metabolomics analysis	1389:1420	Untargeted metabolomics analysis	1389:1420	Untargeted metabolomics analysis identified several plasma metabolites responsible for discriminating water + LPS group from ABX + LPS group.
35981631	11	62	theme	ABX + LPS	1372:1380	arg1	group					1382:1386	ABX + LPS group	1372:1386	ABX + LPS group	1372:1386	LEfSe analysis identified Enterococusfaecalis, Clostriumtertium, and Bacteroidescaecimyris as potential microbial markers for ABX + LPS group.
35981631	15	63	theme	ABX-induced	1792:1802	arg1	depletion					1815:1823	ABX-induced microbiome depletion	1792:1823	ABX-induced microbiome depletion	1792:1823	SIGNIFICANCE These data suggest that ABX-induced microbiome depletion could protect against LPS-induced ALI via the gut-microbiota-lung axis.
35981631	13	64	theme	plasma	1608:1613	arg1	metabolites					1615:1625	the microbiome and plasma metabolites	1589:1625	the microbiome and plasma metabolites	1589:1625	There were correlations between the relative abundance of the microbiome and plasma metabolites.
35981631	3	65	theme	axis	305:308	arg1	role					278:281	the role	274:281	the role of the gut-microbiota axis in the pathology of lung injury	274:340	An increasing evidence suggests the role of the gut-microbiota axis in the pathology of lung injury.
35981631	14	66	theme	IL-6	1685:1688	arg1	levels					1690:1695	IL-6 levels	1685:1695	IL-6 levels in BALF	1685:1703	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	10	67	theme	group	1219:1223	arg1	α-					1181:1182	α-	1181:1182	α-	1181:1182	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	10	67	theme	group	1219:1223	arg1	β-diversity					1188:1198	β-diversity	1188:1198	β-diversity	1188:1198	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	0	68	theme	gut-lung	83:90	arg1	axis					92:95	gut-lung axis	83:95	gut-lung axis	83:95	Antibiotic-induced microbiome depletion improves LPS-induced acute lung injury via gut-lung axis.
35981631	13	69	theme	microbiome	1593:1602	arg1	metabolites					1615:1625	the microbiome and plasma metabolites	1589:1625	the microbiome and plasma metabolites	1589:1625	There were correlations between the relative abundance of the microbiome and plasma metabolites.
35981631	5	70	theme	antibiotic	523:532	arg1	cocktail					534:541	antibiotic cocktail	523:541	antibiotic cocktail (ABX)	523:547	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	5	70	theme	antibiotic	523:532	arg1	ABX					544:546	ABX	544:546	ABX	544:546	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	1	71	theme	inflammatory	139:150	arg1	AIMS					98:101	AIMS Acute lung injury (ALI)	98:125	AIMS Acute lung injury (ALI)	98:125	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	1	71	theme	inflammatory	139:150	arg1	disorder					152:159	an acute inflammatory disorder	130:159	an acute inflammatory disorder	130:159	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	14	72	theme	Integrative	1628:1638	arg1	analysis					1648:1655	Integrative network analysis	1628:1655	Integrative network analysis	1628:1655	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	10	73	theme	ABX + LPS	1229:1237	arg1	group					1239:1243	ABX + LPS group	1229:1243	ABX + LPS group	1229:1243	There were significant differences in the α- and β-diversity of the water + LPS group and ABX + LPS group.
35981631	6	74	from	analysis	659:666	arg1	samples					720:726	feces samples	714:726	feces samples	714:726	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	6	75	theme	fatty	699:703	arg1	acids					705:709	short-chain fatty acids	687:709	short-chain fatty acids in feces samples	687:726	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	9	76	theme	ABX	1051:1053	arg1	treatment					1066:1074	ABX and/or LPS treatment	1051:1074	treatment	1066:1074	Furthermore, ABX and/or LPS treatment markedly altered the α- and β-diversity of the gut microbiota.
35981631	5	77	theme	LPS	608:610	arg1	administration					590:603	intratracheally administration	574:603	intratracheally administration of LPS (5 mg/kg)	574:620	MAIN METHODS The effects of antibiotic cocktail (ABX) on ALI in the mice after intratracheally administration of LPS (5 mg/kg) were examined.
35981631	3	78	theme	injury	335:340	arg1	pathology					317:325	the pathology	313:325	the pathology of lung injury	313:340	An increasing evidence suggests the role of the gut-microbiota axis in the pathology of lung injury.
35981631	9	79	theme	LPS	1062:1064	arg1	treatment					1066:1074	ABX and/or LPS treatment	1051:1074	treatment	1066:1074	Furthermore, ABX and/or LPS treatment markedly altered the α- and β-diversity of the gut microbiota.
35981631	0	80	theme	microbiome	19:28	arg1	depletion					30:38	Antibiotic-induced microbiome depletion	0:38	Antibiotic-induced microbiome depletion	0:38	Antibiotic-induced microbiome depletion improves LPS-induced acute lung injury via gut-lung axis.
35981631	14	81	theme	several	1709:1715	arg1	metabolites					1741:1751	plasma metabolites	1734:1751	plasma metabolites	1734:1751	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	14	81	theme	several	1709:1715	arg1	microbes					1721:1728	several gut microbes	1709:1728	several gut microbes (or plasma metabolites)	1709:1752	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	15	82	theme	gut-microbiota-lung	1871:1889	arg1	axis					1891:1894	the gut-microbiota-lung axis	1867:1894	the gut-microbiota-lung axis	1867:1894	SIGNIFICANCE These data suggest that ABX-induced microbiome depletion could protect against LPS-induced ALI via the gut-microbiota-lung axis.
35981631	7	83	theme	water-treated	906:918	arg1	mice					920:923	water-treated mice	906:923	water-treated mice	906:923	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	1	84	theme	lung	109:112	arg1	ALI					122:124	ALI	122:124	ALI	122:124	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	1	84	theme	lung	109:112	arg1	injury					114:119	Acute lung injury	103:119	AIMS Acute lung injury (ALI)	98:125	AIMS Acute lung injury (ALI) is an acute inflammatory disorder.
35981631	9	85	theme	microbiota	1127:1136	arg1	β-diversity					1104:1114	β-diversity	1104:1114	β-diversity	1104:1114	Furthermore, ABX and/or LPS treatment markedly altered the α- and β-diversity of the gut microbiota.
35981631	9	85	theme	microbiota	1127:1136	arg1	α-					1097:1098	α-	1097:1098	α-	1097:1098	Furthermore, ABX and/or LPS treatment markedly altered the α- and β-diversity of the gut microbiota.
35981631	6	86	theme	rRNA	654:657	arg1	analysis					659:666	16s rRNA analysis	650:666	16s rRNA analysis	650:666	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	0	87	theme	acute	61:65	arg1	injury					72:77	LPS-induced acute lung injury	49:77	LPS-induced acute lung injury	49:77	Antibiotic-induced microbiome depletion improves LPS-induced acute lung injury via gut-lung axis.
35981631	7	88	theme	KEY	787:789	arg1	LPS					800:802	KEY FINDINGS LPS	787:802	KEY FINDINGS LPS	787:802	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	14	89	from	levels	1690:1695	arg1	BALF					1700:1703	BALF	1700:1703	BALF	1700:1703	Integrative network analysis showed correlations between IL-6 levels in BALF and several gut microbes (or plasma metabolites).
35981631	6	90	theme	metabolomics	732:743	arg1	analysis					745:752	metabolomics analysis	732:752	metabolomics analysis of blood samples	732:769	Furthermore, 16s rRNA analysis and measurement of short-chain fatty acids in feces samples and metabolomics analysis of blood samples were performed.
35981631	13	91	theme	metabolites	1615:1625	arg1	abundance					1576:1584	the relative abundance	1563:1584	the relative abundance of the microbiome and plasma metabolites	1563:1625	There were correlations between the relative abundance of the microbiome and plasma metabolites.
35981631	9	92	theme	gut	1123:1125	arg1	microbiota					1127:1136	the gut microbiota	1119:1136	the gut microbiota	1119:1136	Furthermore, ABX and/or LPS treatment markedly altered the α- and β-diversity of the gut microbiota.
35981631	9	93	dep	α-	1097:1098	arg1	the					1093:1095	the	1093:1095	the	1093:1095	Furthermore, ABX and/or LPS treatment markedly altered the α- and β-diversity of the gut microbiota.
35981631	7	94	from	levels	853:858	arg1	BALF					897:900	BALF	897:900	BALF	897:900	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	7	94	from	levels	853:858	arg1	fluid					890:894	the bronchoalveolar lavage fluid	863:894	the bronchoalveolar lavage fluid (BALF) of water-treated mice	863:923	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	7	95	theme	bronchoalveolar	867:881	arg1	BALF					897:900	BALF	897:900	BALF	897:900	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
35981631	7	95	theme	bronchoalveolar	867:881	arg1	fluid					890:894	the bronchoalveolar lavage fluid	863:894	the bronchoalveolar lavage fluid (BALF) of water-treated mice	863:923	KEY FINDINGS LPS significantly increased the interleukin-6 (IL-6) levels in the bronchoalveolar lavage fluid (BALF) of water-treated mice.
36941715	6	0	theme	hUC-EVs	1023:1029	arg1	infusion					1031:1038	hUC-EVs infusion	1023:1038	hUC-EVs infusion	1023:1038	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	1	1	theme	cell	261:264	arg1	lines					266:270	the most widely investigated cell lines	232:270	the most widely investigated cell lines	232:270	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	14	2	theme	wall	2392:2395	arg1	structure					2373:2381	the structure	2369:2381	the structure of colon wall in mice	2369:2403	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	10	3	theme	Th17	1816:1819	arg1	cells					1821:1825	Treg and Th17 cells	1807:1825	cells	1821:1825	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	5	4	theme	hUC-Exos	672:679	arg1	effects					661:667	the therapeutic effects	645:667	the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model	645:731	In this study, the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model were investigated.
36941715	15	5	theme	beneficial	2567:2576	arg1	bacteria					2578:2585	beneficial bacteria	2567:2585	beneficial bacteria	2567:2585	The improvement of intestinal wall structure provides conditions for the reproduction of beneficial bacteria, which further contributes to the reduction of colitis.
36941715	9	6	theme	CD8 + Foxp3 + cells	1546:1564	arg1	number					1518:1523	the number	1514:1523	the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells	1514:1564	We found that the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells increased in order to suppress inflammation, but the length of colon did not recover and the symotoms were worsened of the colonic tissue in DSS14D group.
36941715	4	7	from	differences	579:589	arg1	function					598:605	the function	594:605	the function of secreting exosomes	594:627	However, the heterogeneity of MSCs may lead to differences in the function of secreting exosomes.
36941715	10	8	theme	Treg	1807:1810	arg1	cells					1821:1825	Treg and Th17 cells	1807:1825	cells	1821:1825	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	1	9	theme	mesenchymal	196:206	arg1	MSC					220:222	MSC	220:222	MSC	220:222	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	1	9	theme	mesenchymal	196:206	arg1	cells					213:217	mesenchymal stem cells	196:217	mesenchymal stem cells (MSC)	196:223	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	3	10	attach	derived	453:459	arg1	MSCs					466:469	MSCs	466:469	MSCs	466:469	Exosomes derived from MSCs have shown promising therapeutic potential in many diseases.
36941715	3	10	attach	derived	453:459	arg2	Exosomes					444:451	Exosomes	444:451	Exosomes derived from MSCs	444:469	Exosomes derived from MSCs have shown promising therapeutic potential in many diseases.
36941715	6	11	dep	divided	799:805	arg1	1					826:826	1	826:826	1	826:826	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	10	12	from	transformation	1789:1802	arg1	mice					1838:1841	colitis mice	1830:1841	colitis mice	1830:1841	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	6	13	dep	administered	1073:1084	arg1	4					1066:1066	4	1066:1066	4	1066:1066	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	5	14	theme	colitis	713:719	arg1	model					727:731	the DSS-induced colitis mouse model	697:731	the DSS-induced colitis mouse model	697:731	In this study, the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model were investigated.
36941715	6	15	dep	administered	889:900	arg1	2					882:882	2	882:882	2	882:882	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	5	16	theme	hFP-Exos	685:692	arg1	effects					661:667	the therapeutic effects	645:667	the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model	645:731	In this study, the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model were investigated.
36941715	12	17	theme	intestinal	2201:2210	arg1	mucosa					2212:2217	the intestinal mucosa	2197:2217	the intestinal mucosa	2197:2217	CONCLUSIONS We found that Foxp3 + Treg cells can inhibit the inflammatory response, and the over-activated Treg cells can still further damage the intestinal mucosa.
36941715	6	18	theme	hFP-EVs	1115:1121	arg1	infusion					1123:1130	hFP-EVs infusion	1115:1130	hFP-EVs infusion	1115:1130	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	9	19	theme	colon	1629:1633	arg1	length					1619:1624	the length	1615:1624	the length of colon	1615:1633	We found that the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells increased in order to suppress inflammation, but the length of colon did not recover and the symotoms were worsened of the colonic tissue in DSS14D group.
36941715	7	20	theme	microbiota	1226:1235	arg1	composition					1237:1247	gut microbiota composition	1222:1247	gut microbiota composition	1222:1247	We analyzed colon length, histopathology, Treg cells, cytokines and gut microbiota composition in each group.
36941715	12	21	dep	CONCLUSIONS	2054:2064	arg1	found					2069:2073	found	2069:2073	found that Foxp3 + Treg cells can inhibit the inflammatory response, and the over-activated Treg cells can still further damage the intestinal mucosa	2069:2217	CONCLUSIONS We found that Foxp3 + Treg cells can inhibit the inflammatory response, and the over-activated Treg cells can still further damage the intestinal mucosa.
36941715	0	22	theme	Foxp3 + Treg	79:90	arg1	cells					92:96	the Foxp3 + Treg cells	75:96	the Foxp3 + Treg cells	75:96	Perinatal tissue-derived exosomes ameliorate colitis in mice by regulating the Foxp3 + Treg cells and gut microbiota.
36941715	1	23	theme	differentiation	174:188	arg1	capacity					133:140	The capacity	129:140	The capacity of self-renewal and multipotent differentiation	129:188	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	8	24	theme	CD4 + Foxp3 + and	1367:1383	arg1	CD8 + Foxp3 + cells					1385:1403	CD4 + Foxp3 + and CD8 + Foxp3 + cells	1367:1403	CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group	1367:1418	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	12	25	theme	over-activated	2146:2159	arg1	cells					2166:2170	the over-activated Treg cells	2142:2170	the over-activated Treg cells	2142:2170	CONCLUSIONS We found that Foxp3 + Treg cells can inhibit the inflammatory response, and the over-activated Treg cells can still further damage the intestinal mucosa.
36941715	14	26	from	structure	2373:2381	arg1	mice					2400:2403	mice	2400:2403	mice	2400:2403	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	2	27	theme	therapeutic	419:429	arg1	application					431:441	the therapeutic application	415:441	the therapeutic application	415:441	However, the low survival rate and poor homing efficiency of MSCs after transplantation hinder the therapeutic application.
36941715	1	28	dep	BACKGROUND	118:127	arg1	makes					190:194	makes	190:194	makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies	190:317	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	7	29	theme	gut	1222:1224	arg1	composition					1237:1247	gut microbiota composition	1222:1247	gut microbiota composition	1222:1247	We analyzed colon length, histopathology, Treg cells, cytokines and gut microbiota composition in each group.
36941715	0	30	from	colitis	45:51	arg1	mice					56:59	mice	56:59	mice	56:59	Perinatal tissue-derived exosomes ameliorate colitis in mice by regulating the Foxp3 + Treg cells and gut microbiota.
36941715	2	31	theme	MSCs	381:384	arg1	rate					346:349	the low survival rate	329:349	the low survival rate	329:349	However, the low survival rate and poor homing efficiency of MSCs after transplantation hinder the therapeutic application.
36941715	2	31	theme	MSCs	381:384	arg1	efficiency					367:376	poor homing efficiency	355:376	poor homing efficiency of MSCs after transplantation	355:406	However, the low survival rate and poor homing efficiency of MSCs after transplantation hinder the therapeutic application.
36941715	0	32	theme	Perinatal	0:8	arg1	exosomes					25:32	Perinatal tissue-derived exosomes	0:32	Perinatal tissue-derived exosomes	0:32	Perinatal tissue-derived exosomes ameliorate colitis in mice by regulating the Foxp3 + Treg cells and gut microbiota.
36941715	1	33	theme	preclinical	275:285	arg1	studies					287:293	preclinical studies	275:293	preclinical studies as cell-based therapies	275:317	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	13	34	theme	Treg	2316:2319	arg1	cells					2321:2325	Treg cells	2316:2325	Treg cells	2316:2325	hUC-Exos and hFP-Exos can control inflammation by regulating the balance between Th17 cells and Treg cells.
36941715	12	35	theme	inflammatory	2115:2126	arg1	response					2128:2135	the inflammatory response	2111:2135	the inflammatory response	2111:2135	CONCLUSIONS We found that Foxp3 + Treg cells can inhibit the inflammatory response, and the over-activated Treg cells can still further damage the intestinal mucosa.
36941715	8	36	from	number	1357:1362	arg1	group					1414:1418	DSS7D group	1408:1418	DSS7D group	1408:1418	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	10	37	theme	subsequent	1725:1734	arg1	infusion					1736:1743	The subsequent infusion	1721:1743	The subsequent infusion of either hUC-Exos or hFP-Exos	1721:1774	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	8	38	from	group	1414:1418	arg1	number					1357:1362	the number	1353:1362	the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group	1353:1418	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	14	39	theme	bacteria	2451:2458	arg1	abundance					2421:2429	the abundance	2417:2429	the abundance of pro-inflammatory bacteria in the intestine	2417:2475	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	9	40	theme	DSS14D	1707:1712	arg1	group					1714:1718	DSS14D group	1707:1718	DSS14D group	1707:1718	We found that the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells increased in order to suppress inflammation, but the length of colon did not recover and the symotoms were worsened of the colonic tissue in DSS14D group.
36941715	8	41	theme	IL-6	1290:1293	arg1	IL-17					1296:1300	IL-17	1296:1300	IL-17	1296:1300	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	41	theme	IL-6	1290:1293	arg1	IL-6					1290:1293	IL-6	1290:1293	IL-6	1290:1293	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	41	theme	IL-6	1290:1293	arg1	RESULTS					1264:1270	RESULTS	1264:1270	RESULTS A large amount of IL-6, IL-17 and IFN-γ	1264:1310	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	41	theme	IL-6	1290:1293	arg1	amount					1280:1285	A large amount	1272:1285	A large amount of IL-6	1272:1293	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	41	theme	IL-6	1290:1293	arg1	IFN-γ					1306:1310	IFN-γ	1306:1310	IFN-γ	1306:1310	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	6	42	theme	mouse	772:776	arg1	models					778:783	The colitis mouse models	760:783	The colitis mouse models	760:783	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	10	43	theme	hUC-Exos	1755:1762	arg1	infusion					1736:1743	The subsequent infusion	1721:1743	The subsequent infusion of either hUC-Exos or hFP-Exos	1721:1774	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	14	44	theme	Decreased	2328:2336	arg1	response					2351:2358	Decreased inflammatory response	2328:2358	Decreased inflammatory response	2328:2358	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	2	45	theme	low	333:335	arg1	rate					346:349	the low survival rate	329:349	the low survival rate	329:349	However, the low survival rate and poor homing efficiency of MSCs after transplantation hinder the therapeutic application.
36941715	15	46	theme	structure	2513:2521	arg1	improvement					2482:2492	The improvement	2478:2492	The improvement of intestinal wall structure	2478:2521	The improvement of intestinal wall structure provides conditions for the reproduction of beneficial bacteria, which further contributes to the reduction of colitis.
36941715	7	47	theme	colon	1166:1170	arg1	length					1172:1177	colon length	1166:1177	colon length	1166:1177	We analyzed colon length, histopathology, Treg cells, cytokines and gut microbiota composition in each group.
36941715	6	48	dep	METHODS	752:758	arg1	divided					799:805	divided	799:805	were randomly divided into four groups	785:822	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	4	49	theme	secreting	610:618	arg1	exosomes					620:627	secreting exosomes	610:627	secreting exosomes	610:627	However, the heterogeneity of MSCs may lead to differences in the function of secreting exosomes.
36941715	8	50	dep	RESULTS	1264:1270	arg1	IL-17					1296:1300	IL-17	1296:1300	IL-17	1296:1300	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	50	dep	RESULTS	1264:1270	arg1	IL-6					1290:1293	IL-6	1290:1293	IL-6	1290:1293	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	50	dep	RESULTS	1264:1270	arg1	RESULTS					1264:1270	RESULTS	1264:1270	RESULTS A large amount of IL-6, IL-17 and IFN-γ	1264:1310	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	50	dep	RESULTS	1264:1270	arg1	amount					1280:1285	A large amount	1272:1285	A large amount of IL-6	1272:1293	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	50	dep	RESULTS	1264:1270	arg1	IFN-γ					1306:1310	IFN-γ	1306:1310	IFN-γ	1306:1310	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	5	51	theme	therapeutic	649:659	arg1	effects					661:667	the therapeutic effects	645:667	the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model	645:731	In this study, the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model were investigated.
36941715	2	52	theme	poor	355:358	arg1	efficiency					367:376	poor homing efficiency	355:376	poor homing efficiency of MSCs after transplantation	355:406	However, the low survival rate and poor homing efficiency of MSCs after transplantation hinder the therapeutic application.
36941715	1	53	theme	investigated	248:259	arg1	lines					266:270	the most widely investigated cell lines	232:270	the most widely investigated cell lines	232:270	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	9	54	from	tissue	1697:1702	arg1	group					1714:1718	DSS14D group	1707:1718	DSS14D group	1707:1718	We found that the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells increased in order to suppress inflammation, but the length of colon did not recover and the symotoms were worsened of the colonic tissue in DSS14D group.
36941715	10	55	theme	hFP-Exos	1767:1774	arg1	infusion					1736:1743	The subsequent infusion	1721:1743	The subsequent infusion of either hUC-Exos or hFP-Exos	1721:1774	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	1	56	theme	lines	266:270	arg1	lines					266:270	the most widely investigated cell lines	232:270	the most widely investigated cell lines	232:270	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	1	56	theme	lines	266:270	arg1	one					225:227	one	225:227	one	225:227	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	14	57	theme	colon	2386:2390	arg1	wall					2392:2395	colon wall	2386:2395	colon wall	2386:2395	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	9	58	theme	CD4 + Foxp3 + and	1528:1544	arg1	CD8 + Foxp3 + cells					1546:1564	CD4 + Foxp3 + and CD8 + Foxp3 + cells	1528:1564	CD4 + Foxp3 + and CD8 + Foxp3 + cells	1528:1564	We found that the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells increased in order to suppress inflammation, but the length of colon did not recover and the symotoms were worsened of the colonic tissue in DSS14D group.
36941715	0	59	link	tissue-derived	10:23	arg1	exosomes					25:32	Perinatal tissue-derived exosomes	0:32	Perinatal tissue-derived exosomes	0:32	Perinatal tissue-derived exosomes ameliorate colitis in mice by regulating the Foxp3 + Treg cells and gut microbiota.
36941715	15	60	theme	bacteria	2578:2585	arg1	reproduction					2551:2562	the reproduction	2547:2562	the reproduction of beneficial bacteria	2547:2585	The improvement of intestinal wall structure provides conditions for the reproduction of beneficial bacteria, which further contributes to the reduction of colitis.
36941715	3	61	from	potential	504:512	arg1	diseases					522:529	many diseases	517:529	many diseases	517:529	Exosomes derived from MSCs have shown promising therapeutic potential in many diseases.
36941715	11	62	theme	hFP-Exos	1900:1907	arg1	infusion					1875:1882	The infusion	1871:1882	The infusion of hUC-Exos and hFP-Exos	1871:1907	The infusion of hUC-Exos and hFP-Exos also both reduced the abundance of pro-inflammatory intestinal bacterial such as Verrucomicrobia and Akkermansia muciniphila to improve colitis.
36941715	1	63	theme	stem	208:211	arg1	MSC					220:222	MSC	220:222	MSC	220:222	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	1	63	theme	stem	208:211	arg1	cells					213:217	mesenchymal stem cells	196:217	mesenchymal stem cells (MSC)	196:223	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	11	64	dep	Verrucomicrobia	1990:2004	arg1	muciniphila					2022:2032	muciniphila	2022:2032	muciniphila	2022:2032	The infusion of hUC-Exos and hFP-Exos also both reduced the abundance of pro-inflammatory intestinal bacterial such as Verrucomicrobia and Akkermansia muciniphila to improve colitis.
36941715	10	65	theme	colitis	1830:1836	arg1	mice					1838:1841	colitis mice	1830:1841	colitis mice	1830:1841	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	5	66	theme	DSS-induced	701:711	arg1	model					727:731	the DSS-induced colitis mouse model	697:731	the DSS-induced colitis mouse model	697:731	In this study, the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model were investigated.
36941715	11	67	theme	hUC-Exos	1887:1894	arg1	infusion					1875:1882	The infusion	1871:1882	The infusion of hUC-Exos and hFP-Exos	1871:1907	The infusion of hUC-Exos and hFP-Exos also both reduced the abundance of pro-inflammatory intestinal bacterial such as Verrucomicrobia and Akkermansia muciniphila to improve colitis.
36941715	14	68	theme	inflammatory	2338:2349	arg1	response					2351:2358	Decreased inflammatory response	2328:2358	Decreased inflammatory response	2328:2358	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	10	69	theme	cells	1821:1825	arg1	transformation					1789:1802	the transformation	1785:1802	the transformation of Treg and Th17 cells in colitis mice	1785:1841	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	5	70	theme	mouse	721:725	arg1	model					727:731	the DSS-induced colitis mouse model	697:731	the DSS-induced colitis mouse model	697:731	In this study, the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model were investigated.
36941715	10	71	theme	immune	1855:1860	arg1	balance					1862:1868	immune balance	1855:1868	immune balance	1855:1868	The subsequent infusion of either hUC-Exos or hFP-Exos mediated the transformation of Treg and Th17 cells in colitis mice to maintain immune balance.
36941715	11	72	theme	bacterial	1972:1980	arg1	abundance					1931:1939	the abundance	1927:1939	the abundance of pro-inflammatory intestinal bacterial such as Verrucomicrobia and Akkermansia muciniphila to improve colitis	1927:2051	The infusion of hUC-Exos and hFP-Exos also both reduced the abundance of pro-inflammatory intestinal bacterial such as Verrucomicrobia and Akkermansia muciniphila to improve colitis.
36941715	3	73	theme	therapeutic	492:502	arg1	potential					504:512	promising therapeutic potential	482:512	promising therapeutic potential in many diseases	482:529	Exosomes derived from MSCs have shown promising therapeutic potential in many diseases.
36941715	2	74	theme	survival	337:344	arg1	rate					346:349	the low survival rate	329:349	the low survival rate	329:349	However, the low survival rate and poor homing efficiency of MSCs after transplantation hinder the therapeutic application.
36941715	4	75	gly	heterogeneity	545:557	arg1	MSCs					562:565	MSCs	562:565	MSCs	562:565	However, the heterogeneity of MSCs may lead to differences in the function of secreting exosomes.
36941715	1	76	theme	self-renewal	145:156	arg1	differentiation					174:188	self-renewal and multipotent differentiation	145:188	self-renewal and multipotent differentiation	145:188	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	1	77	theme	multipotent	162:172	arg1	differentiation					174:188	self-renewal and multipotent differentiation	145:188	self-renewal and multipotent differentiation	145:188	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	12	78	theme	Treg	2161:2164	arg1	cells					2166:2170	the over-activated Treg cells	2142:2170	the over-activated Treg cells	2142:2170	CONCLUSIONS We found that Foxp3 + Treg cells can inhibit the inflammatory response, and the over-activated Treg cells can still further damage the intestinal mucosa.
36941715	8	79	theme	CD8 + Foxp3 + cells	1385:1403	arg1	number					1357:1362	the number	1353:1362	the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group	1353:1418	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	0	80	theme	gut	102:104	arg1	microbiota					106:115	gut microbiota	102:115	gut microbiota	102:115	Perinatal tissue-derived exosomes ameliorate colitis in mice by regulating the Foxp3 + Treg cells and gut microbiota.
36941715	8	81	theme	DSS7D	1408:1412	arg1	group					1414:1418	DSS7D group	1408:1418	DSS7D group	1408:1418	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	82	from	decrease	1341:1348	arg1	number					1357:1362	the number	1353:1362	the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group	1353:1418	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	83	theme	Treg	1472:1475	arg1	cells					1477:1481	Treg cells	1472:1481	Treg cells	1472:1481	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	0	84	theme	tissue-derived	10:23	arg1	exosomes					25:32	Perinatal tissue-derived exosomes	0:32	Perinatal tissue-derived exosomes	0:32	Perinatal tissue-derived exosomes ameliorate colitis in mice by regulating the Foxp3 + Treg cells and gut microbiota.
36941715	7	85	theme	Treg	1196:1199	arg1	cells					1201:1205	Treg cells	1196:1205	Treg cells	1196:1205	We analyzed colon length, histopathology, Treg cells, cytokines and gut microbiota composition in each group.
36941715	9	86	theme	colonic	1689:1695	arg1	tissue					1697:1702	the colonic tissue	1685:1702	the colonic tissue in DSS14D group	1685:1718	We found that the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells increased in order to suppress inflammation, but the length of colon did not recover and the symotoms were worsened of the colonic tissue in DSS14D group.
36941715	8	87	theme	Th17	1442:1445	arg1	cells					1447:1451	Th17 cells	1442:1451	Th17 cells	1442:1451	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	6	88	dep	administered	981:992	arg1	3					974:974	3	974:974	3	974:974	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	3	89	theme	promising	482:490	arg1	potential					504:512	promising therapeutic potential	482:512	promising therapeutic potential in many diseases	482:529	Exosomes derived from MSCs have shown promising therapeutic potential in many diseases.
36941715	1	90	theme	cell-based	298:307	arg1	therapies					309:317	cell-based therapies	298:317	cell-based therapies	298:317	BACKGROUND The capacity of self-renewal and multipotent differentiation makes mesenchymal stem cells (MSC) one of the most widely investigated cell lines in preclinical studies as cell-based therapies.
36941715	15	91	theme	intestinal	2497:2506	arg1	structure					2513:2521	intestinal wall structure	2497:2521	intestinal wall structure	2497:2521	The improvement of intestinal wall structure provides conditions for the reproduction of beneficial bacteria, which further contributes to the reduction of colitis.
36941715	8	92	theme	large	1274:1278	arg1	IL-6					1290:1293	IL-6	1290:1293	IL-6	1290:1293	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	92	theme	large	1274:1278	arg1	RESULTS					1264:1270	RESULTS	1264:1270	RESULTS A large amount of IL-6, IL-17 and IFN-γ	1264:1310	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	8	92	theme	large	1274:1278	arg1	amount					1280:1285	A large amount	1272:1285	A large amount of IL-6	1272:1293	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
36941715	15	93	theme	colitis	2634:2640	arg1	reduction					2621:2629	the reduction	2617:2629	the reduction of colitis	2617:2640	The improvement of intestinal wall structure provides conditions for the reproduction of beneficial bacteria, which further contributes to the reduction of colitis.
36941715	14	94	theme	pro-inflammatory	2434:2449	arg1	bacteria					2451:2458	pro-inflammatory bacteria	2434:2458	pro-inflammatory bacteria	2434:2458	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	13	95	theme	Th17	2301:2304	arg1	cells					2306:2310	Th17 cells	2301:2310	Th17 cells	2301:2310	hUC-Exos and hFP-Exos can control inflammation by regulating the balance between Th17 cells and Treg cells.
36941715	4	96	theme	MSCs	562:565	arg1	heterogeneity					545:557	the heterogeneity	541:557	the heterogeneity of MSCs	541:565	However, the heterogeneity of MSCs may lead to differences in the function of secreting exosomes.
36941715	12	97	theme	Foxp3 + Treg	2080:2091	arg1	cells					2093:2097	Foxp3 + Treg cells	2080:2097	Foxp3 + Treg cells	2080:2097	CONCLUSIONS We found that Foxp3 + Treg cells can inhibit the inflammatory response, and the over-activated Treg cells can still further damage the intestinal mucosa.
36941715	6	98	theme	colitis	764:770	arg1	models					778:783	The colitis mouse models	760:783	The colitis mouse models	760:783	METHODS The colitis mouse models were randomly divided into four groups: (1) DSS administered for 7 days and euthanasia (DSS7D), (2) DSS administered for 7 days and kept for another 7 days without any treatment (DSS14D), (3) DSS administered for 7 days and followed with hUC-EVs infusion for 7 days (hUC-EVs) and (4) DSS administered for 7 days and followed with hFP-EVs infusion for 7 days (hFP-EVs).
36941715	3	99	theme	many	517:520	arg1	diseases					522:529	many diseases	517:529	many diseases	517:529	Exosomes derived from MSCs have shown promising therapeutic potential in many diseases.
36941715	15	100	theme	wall	2508:2511	arg1	structure					2513:2521	intestinal wall structure	2497:2521	intestinal wall structure	2497:2521	The improvement of intestinal wall structure provides conditions for the reproduction of beneficial bacteria, which further contributes to the reduction of colitis.
36941715	5	101	from	effects	661:667	arg1	model					727:731	the DSS-induced colitis mouse model	697:731	the DSS-induced colitis mouse model	697:731	In this study, the therapeutic effects of hUC-Exos and hFP-Exos on the DSS-induced colitis mouse model were investigated.
36941715	4	102	theme	exosomes	620:627	arg1	function					598:605	the function	594:605	the function of secreting exosomes	594:627	However, the heterogeneity of MSCs may lead to differences in the function of secreting exosomes.
36941715	14	103	from	abundance	2421:2429	arg1	intestine					2467:2475	the intestine	2463:2475	the intestine	2463:2475	Decreased inflammatory response improved the structure of colon wall in mice and reduced the abundance of pro-inflammatory bacteria in the intestine.
36941715	2	104	theme	homing	360:365	arg1	efficiency					367:376	poor homing efficiency	355:376	poor homing efficiency of MSCs after transplantation	355:406	However, the low survival rate and poor homing efficiency of MSCs after transplantation hinder the therapeutic application.
36941715	11	105	dep	reduced	1919:1925	arg1	both					1914:1917	both	1914:1917	both	1914:1917	The infusion of hUC-Exos and hFP-Exos also both reduced the abundance of pro-inflammatory intestinal bacterial such as Verrucomicrobia and Akkermansia muciniphila to improve colitis.
36941715	8	106	from	CD8 + Foxp3 + cells	1385:1403	arg1	group					1414:1418	DSS7D group	1408:1418	DSS7D group	1408:1418	RESULTS A large amount of IL-6, IL-17 and IFN-γ were produced along with the decrease in the number of CD4 + Foxp3 + and CD8 + Foxp3 + cells in DSS7D group, which indicated that Th17 cells were activated and Treg cells were suppressed.
37297405	7	0	theme	broad	1172:1176	arg1	beans					1178:1182	freeze-dried and sun-dried broad beans	1145:1182	freeze-dried and sun-dried broad beans	1145:1182	Notably, freeze-dried and sun-dried broad beans exhibited a higher concentration of differential substances.
37297405	3	1	theme	nutritional	460:470	arg1	composition					472:482	nutritional composition	460:482	nutritional composition	460:482	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	1	2	theme	freeze	209:214	arg1	drying					216:221	freeze drying	209:221	freeze drying	209:221	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	1	3	theme	different	141:149	arg1	drying					216:221	freeze drying	209:221	freeze drying	209:221	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	1	3	theme	different	141:149	arg1	drying					185:190	hot air drying	177:190	hot air drying	177:190	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	1	3	theme	different	141:149	arg1	methods					158:164	different drying methods	141:164	different drying methods	141:164	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	1	3	theme	different	141:149	arg1	drying					197:202	sun drying	193:202	sun drying	193:202	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	0	4	theme	Bioactive	89:97	arg1	Substances					99:108	Bioactive Substances	89:108	Bioactive Substances	89:108	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	0	5	from	Effects	0:6	arg1	Compounds					74:82	Volatile Flavor Compounds	58:82	Volatile Flavor Compounds	58:82	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	0	5	from	Effects	0:6	arg1	Substances					99:108	Bioactive Substances	89:108	Bioactive Substances	89:108	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	0	5	from	Effects	0:6	arg1	Compositions					44:55	Nutritional Compositions	32:55	Nutritional Compositions	32:55	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	5	6	theme	sun	909:911	arg1	drying					913:918	sun drying	909:918	sun drying	909:918	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	4	7	theme	volatile	552:559	arg1	compounds					569:577	the 66 identified volatile organic compounds	534:577	the 66 identified volatile organic compounds	534:577	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	7	8	theme	substances	1233:1242	arg1	concentration					1203:1215	a higher concentration	1194:1215	a higher concentration of differential substances	1194:1242	Notably, freeze-dried and sun-dried broad beans exhibited a higher concentration of differential substances.
37297405	6	9	from	compounds	974:982	arg1	beans					993:997	broad beans	987:997	broad beans dried by three different methods	987:1030	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	3	10	theme	soluble	505:511	arg1	content					519:525	soluble sugar content	505:525	soluble sugar content	505:525	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	4	11	theme	identified	541:550	arg1	compounds					569:577	the 66 identified volatile organic compounds	534:577	the 66 identified volatile organic compounds	534:577	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	5	12	theme	antioxidant	859:869	arg1	capacity					871:878	antioxidant capacity	859:878	antioxidant capacity	859:878	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	3	13	theme	p	447:447	arg1	differences					434:444	significant differences	422:444	significant differences (p < 0.05)	422:455	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	3	13	theme	p	447:447	arg1	<					449:449	p < 0.05	447:454	p < 0.05	447:454	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	3	13	theme	p	447:447	arg1	protein					493:499	protein	493:499	protein	493:499	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	3	13	theme	p	447:447	arg1	content					519:525	soluble sugar content	505:525	soluble sugar content	505:525	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	1	14	theme	drying	151:156	arg1	drying					216:221	freeze drying	209:221	freeze drying	209:221	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	1	14	theme	drying	151:156	arg1	drying					185:190	hot air drying	177:190	hot air drying	177:190	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	1	14	theme	drying	151:156	arg1	methods					158:164	different drying methods	141:164	different drying methods	141:164	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	1	14	theme	drying	151:156	arg1	drying					197:202	sun drying	193:202	sun drying	193:202	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	6	15	theme	chemometric	925:935	arg1	analysis					937:944	The chemometric analysis	921:944	The chemometric analysis	921:944	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	5	16	theme	strongest	849:857	arg1	capacity					871:878	antioxidant capacity	859:878	antioxidant capacity	859:878	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	5	17	theme	bioactive	736:744	arg1	substances					746:755	bioactive substances	736:755	bioactive substances	736:755	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	5	18	dep	capacity	871:878	arg1	the					845:847	the	845:847	the	845:847	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	2	19	theme	dried	352:356	arg1	beans					364:368	the dried broad beans	348:368	the dried broad beans	348:368	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	2	20	theme	nutritional	267:277	arg1	composition					279:289	The nutritional composition	263:289	The nutritional composition	263:289	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	6	21	theme	different	1014:1022	arg1	methods					1024:1030	three different methods	1008:1030	three different methods	1008:1030	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	5	22	theme	total	813:817	arg1	content					826:832	the highest total phenol content	801:832	the highest total phenol content as well as the strongest antioxidant capacity and gallic acid	801:894	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	0	23	theme	Treatments	18:27	arg1	Effects					0:6	Effects	0:6	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans	0:123	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	0	24	theme	Beans	119:123	arg1	Compounds					74:82	Volatile Flavor Compounds	58:82	Volatile Flavor Compounds	58:82	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	0	24	theme	Beans	119:123	arg1	Substances					99:108	Bioactive Substances	89:108	Bioactive Substances	89:108	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	0	24	theme	Beans	119:123	arg1	Compositions					44:55	Nutritional Compositions	32:55	Nutritional Compositions	32:55	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	3	25	theme	sugar	513:517	arg1	content					519:525	soluble sugar content	505:525	soluble sugar content	505:525	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	1	26	theme	hot	177:179	arg1	drying					185:190	hot air drying	177:190	hot air drying	177:190	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	0	27	theme	Drying	11:16	arg1	Treatments					18:27	Drying Treatments	11:27	Drying Treatments	11:27	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	1	28	theme	fresh	244:248	arg1	beans					256:260	fresh broad beans	244:260	fresh broad beans	244:260	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	0	29	theme	Broad	113:117	arg1	Beans					119:123	Broad Beans	113:123	Broad Beans	113:123	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	4	30	theme	alcohols	653:660	arg1	production					639:648	the production	635:648	the production of alcohols and aldehydes	635:674	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	1	31	theme	air	181:183	arg1	drying					185:190	hot air drying	177:190	hot air drying	177:190	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	0	32	theme	Nutritional	32:42	arg1	Compositions					44:55	Nutritional Compositions	32:55	Nutritional Compositions	32:55	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	1	33	theme	broad	250:254	arg1	beans					256:260	fresh broad beans	244:260	fresh broad beans	244:260	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	4	34	theme	air	602:604	arg1	drying					606:611	hot air drying	598:611	hot air drying	598:611	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	3	35	theme	significant	422:432	arg1	differences					434:444	significant differences	422:444	significant differences (p < 0.05)	422:455	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	3	35	theme	significant	422:432	arg1	<					449:449	p < 0.05	447:454	p < 0.05	447:454	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	3	35	theme	significant	422:432	arg1	protein					493:499	protein	493:499	protein	493:499	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	3	35	theme	significant	422:432	arg1	content					519:525	soluble sugar content	505:525	soluble sugar content	505:525	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	2	36	theme	broad	358:362	arg1	beans					364:368	the dried broad beans	348:368	the dried broad beans	348:368	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	6	37	theme	organic	1071:1077	arg1	acids					1079:1083	organic acids	1071:1083	organic acids	1071:1083	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	5	38	theme	substances	746:755	arg1	terms					727:731	terms	727:731	terms of bioactive substances	727:755	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	6	39	theme	bioactive	964:972	arg1	compounds					974:982	the bioactive compounds	960:982	the bioactive compounds in broad beans dried by three different methods	960:1030	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	5	40	theme	highest	805:811	arg1	content					826:832	the highest total phenol content	801:832	the highest total phenol content as well as the strongest antioxidant capacity and gallic acid	801:894	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	2	41	theme	beans	364:368	arg1	composition					279:289	The nutritional composition	263:289	The nutritional composition	263:289	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	2	41	theme	beans	364:368	arg1	components					309:318	volatile organic components	292:318	volatile organic components	292:318	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	2	41	theme	beans	364:368	arg1	substances					334:343	bioactive substances	324:343	bioactive substances	324:343	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	6	42	theme	broad	987:991	arg1	beans					993:997	broad beans	987:997	broad beans dried by three different methods	987:1030	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	3	43	from	differences	434:444	arg1	composition					472:482	nutritional composition	460:482	nutritional composition	460:482	The results indicated significant differences (p < 0.05) in nutritional composition, such as protein and soluble sugar content.
37297405	2	44	theme	organic	301:307	arg1	components					309:318	volatile organic components	292:318	volatile organic components	292:318	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	5	45	theme	broad	758:762	arg1	beans					764:768	broad beans	758:768	broad beans dried by freeze drying	758:791	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	7	46	theme	freeze-dried	1145:1156	arg1	beans					1178:1182	freeze-dried and sun-dried broad beans	1145:1182	freeze-dried and sun-dried broad beans	1145:1182	Notably, freeze-dried and sun-dried broad beans exhibited a higher concentration of differential substances.
37297405	7	47	theme	sun-dried	1162:1170	arg1	beans					1178:1182	freeze-dried and sun-dried broad beans	1145:1182	freeze-dried and sun-dried broad beans	1145:1182	Notably, freeze-dried and sun-dried broad beans exhibited a higher concentration of differential substances.
37297405	2	48	theme	volatile	292:299	arg1	components					309:318	volatile organic components	292:318	volatile organic components	292:318	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	7	49	theme	differential	1220:1231	arg1	substances					1233:1242	differential substances	1220:1242	differential substances	1220:1242	Notably, freeze-dried and sun-dried broad beans exhibited a higher concentration of differential substances.
37297405	4	50	theme	sun	683:685	arg1	drying					687:692	sun drying	683:692	sun drying	683:692	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	6	51	theme	amino	1090:1094	arg1	acids					1096:1100	amino acids	1090:1100	amino acids	1090:1100	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	1	52	theme	sun	193:195	arg1	drying					197:202	sun drying	193:202	sun drying	193:202	In this study, different drying methods, including hot air drying, sun drying, and freeze drying were employed to dry fresh broad beans.
37297405	6	53	theme	significant	1107:1117	arg1	differentiation					1119:1133	significant differentiation	1107:1133	significant differentiation	1107:1133	The chemometric analysis revealed that the bioactive compounds in broad beans dried by three different methods were primarily composed of flavonoids, organic acids, and amino acids with significant differentiation.
37297405	5	54	theme	gallic	884:889	arg1	acid					891:894	gallic acid	884:894	gallic acid	884:894	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	4	55	theme	organic	561:567	arg1	compounds					569:577	the 66 identified volatile organic compounds	534:577	the 66 identified volatile organic compounds	534:577	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	0	56	theme	Flavor	67:72	arg1	Compounds					74:82	Volatile Flavor Compounds	58:82	Volatile Flavor Compounds	58:82	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	4	57	theme	hot	598:600	arg1	drying					606:611	hot air drying	598:611	hot air drying	598:611	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	5	58	theme	phenol	819:824	arg1	content					826:832	the highest total phenol content	801:832	the highest total phenol content as well as the strongest antioxidant capacity and gallic acid	801:894	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	4	59	theme	freeze	580:585	arg1	drying					587:592	freeze drying	580:592	freeze drying	580:592	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
37297405	0	60	theme	Volatile	58:65	arg1	Compounds					74:82	Volatile Flavor Compounds	58:82	Volatile Flavor Compounds	58:82	Effects of Drying Treatments on Nutritional Compositions, Volatile Flavor Compounds, and Bioactive Substances of Broad Beans.
37297405	5	61	theme	freeze	779:784	arg1	drying					786:791	freeze drying	779:791	freeze drying	779:791	In terms of bioactive substances, broad beans dried by freeze drying exhibit the highest total phenol content as well as the strongest antioxidant capacity and gallic acid, followed by sun drying.
37297405	2	62	theme	bioactive	324:332	arg1	substances					334:343	bioactive substances	324:343	bioactive substances	324:343	The nutritional composition, volatile organic components and bioactive substances of the dried broad beans were systematically compared.
37297405	7	63	theme	higher	1196:1201	arg1	concentration					1203:1215	a higher concentration	1194:1215	a higher concentration of differential substances	1194:1242	Notably, freeze-dried and sun-dried broad beans exhibited a higher concentration of differential substances.
37297405	4	64	theme	aldehydes	666:674	arg1	production					639:648	the production	635:648	the production of alcohols and aldehydes	635:674	Among the 66 identified volatile organic compounds, freeze drying and hot air drying significantly promote the production of alcohols and aldehydes, while sun drying effectively preserves esters.
34791999	6	0	theme	toll-like	890:898	arg1	TLR					910:912	TLR	910:912	TLR	910:912	Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process was also evaluated.
34791999	6	0	theme	toll-like	890:898	arg1	receptor					900:907	toll-like receptor	890:907	toll-like receptor (TLR) knockout mice	890:927	Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process was also evaluated.
34791999	10	1	theme	theoretical	1473:1483	arg1	foundation					1485:1494	a theoretical foundation	1471:1494	a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis	1471:1575	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	2	2	theme	microorganisms	367:380	arg1	composition					342:352	composition	342:352	composition	342:352	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	2	2	theme	microorganisms	367:380	arg1	number					331:336	number	331:336	number	331:336	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	5	3	from	levels	702:707	arg1	cells					723:727	B cells	721:727	B cells	721:727	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	2	4	theme	main	302:305	arg1	feature					307:313	its main feature	298:313	its main feature	298:313	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	10	5	dep	provides	1304:1311	arg1	present					1463:1469	present	1463:1469	present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis	1463:1575	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	10	5	dep	provides	1304:1311	arg1	discussed					1394:1402	discussed	1394:1402	discussed the effect of P. gingivalis on the production of Breg	1394:1456	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	5	6	theme	E.	779:780	arg1	LPS					787:789	E. coli LPS	779:789	E. coli LPS	779:789	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	1	7	theme	present	192:198	arg1	gingivalis					132:141	BACKGROUND Porphyromonas gingivalis	107:141	BACKGROUND Porphyromonas gingivalis (P. gingivalis)	107:157	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	7	theme	present	192:198	arg1	bacterium					175:183	a pathogenic bacterium	162:183	a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis	162:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	8	8	theme	IL-10+Breg	1118:1127	arg1	cell					1129:1132	IL-10+Breg cell	1118:1132	IL-10+Breg cell	1118:1132	CD5+B1 subset is the main source of IL-10+Breg cell.
34791999	9	9	theme	gingivalis	1144:1153	arg1	stimulation					1159:1169	P. gingivalis LPS stimulation	1141:1169	P. gingivalis LPS stimulation	1141:1169	Under P. gingivalis LPS stimulation, CD5+IgM+CD93-IL-10+B cell subset increased significantly, which was regulated through TLR2/ 4.
34791999	1	10	theme	subgingival	203:213	arg1	plaques					215:221	subgingival plaques	203:221	subgingival plaques of patients with periodontitis	203:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	7	11	theme	P.	1020:1021	arg1	gingivalis					1023:1032	P. gingivalis	1020:1032	P. gingivalis	1020:1032	RESULTS Results showed that P. gingivalis stimulated B cells to produce IL-10 via TLR2/4.
34791999	9	12	theme	CD5+IgM+CD93-IL-10+B	1172:1191	arg1	subset					1198:1203	CD5+IgM+CD93-IL-10+B cell subset	1172:1203	CD5+IgM+CD93-IL-10+B cell subset	1172:1203	Under P. gingivalis LPS stimulation, CD5+IgM+CD93-IL-10+B cell subset increased significantly, which was regulated through TLR2/ 4.
34791999	2	13	with	periodontitis	266:278	arg1	loss					290:293	bone loss	285:293	bone loss	285:293	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	7	14	theme	RESULTS	992:998	arg1	Results					1000:1006	RESULTS Results	992:1006	RESULTS Results	992:1006	RESULTS Results showed that P. gingivalis stimulated B cells to produce IL-10 via TLR2/4.
34791999	10	15	dep	CONCLUSION	1267:1276	arg1	provides					1304:1311	provides	1304:1311	provides	1304:1311	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	1	16	theme	P.	144:145	arg1	gingivalis					147:156	P. gingivalis	144:156	P. gingivalis	144:156	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	16	theme	P.	144:145	arg1	gingivalis					132:141	BACKGROUND Porphyromonas gingivalis	107:141	BACKGROUND Porphyromonas gingivalis (P. gingivalis)	107:157	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	2	17	dep	number	331:336	arg1	the					327:329	the	327:329	the	327:329	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	2	18	theme	bone	285:288	arg1	loss					290:293	bone loss	285:293	bone loss	285:293	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	4	19	from	cells	608:612	arg1	development					643:653	development	643:653	development	643:653	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	4	19	from	cells	608:612	arg1	occurrence					628:637	occurrence	628:637	occurrence	628:637	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	5	20	dep	METHODS	673:679	arg1	detected					684:691	detected	684:691	detected	684:691	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	5	21	theme	IL-10	712:716	arg1	levels					702:707	the mRNA levels	693:707	the mRNA levels of IL-10 in B cells	693:727	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	2	22	theme	natural	407:413	arg1	response					422:429	the natural immune response	403:429	the natural immune response of the host	403:441	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	5	23	theme	B	721:721	arg1	cells					723:727	B cells	721:727	B cells	721:727	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	1	24	from	plaques	215:221	arg1	present					192:198	present	192:198	present	192:198	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	10	25	theme	P.	1418:1419	arg1	gingivalis					1421:1430	P. gingivalis	1418:1430	P. gingivalis	1418:1430	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	0	26	theme	Porphyromonas	0:12	arg1	gingivalis					14:23	Porphyromonas gingivalis	0:23	Porphyromonas gingivalis	0:23	Porphyromonas gingivalis Lipopolysaccharide-Induced B Cell Differentiation by Toll-like Receptors 2 and 4.
34791999	1	27	theme	BACKGROUND	107:116	arg1	gingivalis					147:156	P. gingivalis	144:156	P. gingivalis	144:156	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	27	theme	BACKGROUND	107:116	arg1	bacterium					175:183	a pathogenic bacterium	162:183	a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis	162:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	27	theme	BACKGROUND	107:116	arg1	gingivalis					132:141	BACKGROUND Porphyromonas gingivalis	107:141	BACKGROUND Porphyromonas gingivalis (P. gingivalis)	107:157	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	10	28	theme	Breg	1453:1456	arg1	production					1439:1448	the production	1435:1448	the production of Breg	1435:1456	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	5	29	theme	LPS	787:789	arg1	stimulation					739:749	the stimulation	735:749	the stimulation of P. gingivalis LPS and/or E. coli LPS	735:789	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	0	30	dep	Receptors	88:96	arg1	4					104:104	4	104:104	4	104:104	Porphyromonas gingivalis Lipopolysaccharide-Induced B Cell Differentiation by Toll-like Receptors 2 and 4.
34791999	0	30	dep	Receptors	88:96	arg1	2					98:98	2	98:98	2	98:98	Porphyromonas gingivalis Lipopolysaccharide-Induced B Cell Differentiation by Toll-like Receptors 2 and 4.
34791999	4	31	theme	gingivalis	556:565	arg1	effect					543:548	the effect	539:548	the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis	539:670	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	6	32	from	role	934:937	arg1	process					964:970	this process	959:970	this process	959:970	Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process was also evaluated.
34791999	0	33	theme	Cell	54:57	arg1	Differentiation					59:73	B Cell Differentiation	52:73	B Cell Differentiation	52:73	Porphyromonas gingivalis Lipopolysaccharide-Induced B Cell Differentiation by Toll-like Receptors 2 and 4.
34791999	5	34	theme	P.	754:755	arg1	LPS					768:770	P. gingivalis LPS	754:770	P. gingivalis LPS	754:770	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	2	35	theme	symbiotic	357:365	arg1	microorganisms					367:380	symbiotic microorganisms	357:380	symbiotic microorganisms	357:380	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	5	36	theme	LPS	768:770	arg1	stimulation					739:749	the stimulation	735:749	the stimulation of P. gingivalis LPS and/or E. coli LPS	735:789	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	3	37	theme	latter	474:479	arg1	mechanism					457:465	the mechanism	453:465	the mechanism of the latter	453:479	However, the mechanism of the latter remains unclear.
34791999	4	38	theme	regulatory	595:604	arg1	Breg					615:618	Breg	615:618	Breg	615:618	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	4	38	theme	regulatory	595:604	arg1	cells					608:612	regulatory B cells	595:612	regulatory B cells (Breg) in the occurrence and development of periodontitis	595:670	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	10	39	theme	subsequent	1500:1509	arg1	investigations					1511:1524	subsequent investigations	1500:1524	subsequent investigations on the occurrence and development of periodontitis	1500:1575	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	10	40	theme	new	1313:1315	arg1	insights					1317:1324	new insights	1313:1324	new insights into the immunopathogenic mechanism of P. gingivalis	1313:1377	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	5	41	theme	different	833:841	arg1	subgroups					850:858	different B cell subgroups	833:858	different B cell subgroups using flow cytometry	833:879	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	0	42	theme	Toll-like	78:86	arg1	Receptors					88:96	Toll-like Receptors 2 and 4	78:104	Toll-like Receptors 2 and 4	78:104	Porphyromonas gingivalis Lipopolysaccharide-Induced B Cell Differentiation by Toll-like Receptors 2 and 4.
34791999	6	43	theme	receptor	900:907	arg1	mice					924:927	toll-like receptor (TLR) knockout mice	890:927	toll-like receptor (TLR) knockout mice	890:927	Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process was also evaluated.
34791999	4	44	dep	occurrence	628:637	arg1	the					624:626	the	624:626	the	624:626	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	10	45	theme	study	1298:1302	arg1	results					1282:1288	The results	1278:1288	The results of this study	1278:1302	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	8	46	theme	CD5+B1	1082:1087	arg1	subset					1089:1094	CD5+B1 subset	1082:1094	CD5+B1 subset	1082:1094	CD5+B1 subset is the main source of IL-10+Breg cell.
34791999	8	46	theme	CD5+B1	1082:1087	arg1	source					1108:1113	the main source	1099:1113	the main source of IL-10+Breg cell	1099:1132	CD5+B1 subset is the main source of IL-10+Breg cell.
34791999	10	47	theme	gingivalis	1368:1377	arg1	mechanism					1352:1360	the immunopathogenic mechanism	1331:1360	the immunopathogenic mechanism of P. gingivalis	1331:1377	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	10	48	theme	immunopathogenic	1335:1350	arg1	mechanism					1352:1360	the immunopathogenic mechanism	1331:1360	the immunopathogenic mechanism of P. gingivalis	1331:1377	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	5	49	dep	E.	779:780	arg1	coli					782:785	coli	782:785	coli	782:785	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	5	50	theme	flow	866:869	arg1	cytometry					871:879	flow cytometry	866:879	flow cytometry	866:879	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	7	51	theme	B	1045:1045	arg1	cells					1047:1051	B cells	1045:1051	B cells	1045:1051	RESULTS Results showed that P. gingivalis stimulated B cells to produce IL-10 via TLR2/4.
34791999	8	52	theme	main	1103:1106	arg1	subset					1089:1094	CD5+B1 subset	1082:1094	CD5+B1 subset	1082:1094	CD5+B1 subset is the main source of IL-10+Breg cell.
34791999	8	52	theme	main	1103:1106	arg1	source					1108:1113	the main source	1099:1113	the main source of IL-10+Breg cell	1099:1132	CD5+B1 subset is the main source of IL-10+Breg cell.
34791999	10	53	theme	periodontitis	1563:1575	arg1	development					1548:1558	development	1548:1558	development	1548:1558	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	10	53	theme	periodontitis	1563:1575	arg1	occurrence					1533:1542	occurrence	1533:1542	occurrence	1533:1542	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	9	54	theme	P.	1141:1142	arg1	gingivalis					1144:1153	P. gingivalis	1141:1153	P. gingivalis LPS stimulation	1141:1169	Under P. gingivalis LPS stimulation, CD5+IgM+CD93-IL-10+B cell subset increased significantly, which was regulated through TLR2/ 4.
34791999	5	55	theme	B	843:843	arg1	subgroups					850:858	different B cell subgroups	833:858	different B cell subgroups using flow cytometry	833:879	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	10	56	theme	P.	1365:1366	arg1	gingivalis					1368:1377	P. gingivalis	1365:1377	P. gingivalis	1365:1377	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	9	57	theme	LPS	1155:1157	arg1	stimulation					1159:1169	P. gingivalis LPS stimulation	1141:1169	P. gingivalis LPS stimulation	1141:1169	Under P. gingivalis LPS stimulation, CD5+IgM+CD93-IL-10+B cell subset increased significantly, which was regulated through TLR2/ 4.
34791999	8	58	theme	cell	1129:1132	arg1	subset					1089:1094	CD5+B1 subset	1082:1094	CD5+B1 subset	1082:1094	CD5+B1 subset is the main source of IL-10+Breg cell.
34791999	8	58	theme	cell	1129:1132	arg1	source					1108:1113	the main source	1099:1113	the main source of IL-10+Breg cell	1099:1132	CD5+B1 subset is the main source of IL-10+Breg cell.
34791999	10	59	from	effect	1408:1413	arg1	production					1439:1448	the production	1435:1448	the production of Breg	1435:1456	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	10	60	dep	occurrence	1533:1542	arg1	the					1529:1531	the	1529:1531	the	1529:1531	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	5	61	theme	cell	845:848	arg1	subgroups					850:858	different B cell subgroups	833:858	different B cell subgroups using flow cytometry	833:879	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	5	62	theme	IL-10-producing	806:820	arg1	cells					822:826	IL-10-producing cells	806:826	IL-10-producing cells	806:826	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	6	63	theme	knockout	915:922	arg1	mice					924:927	toll-like receptor (TLR) knockout mice	890:927	toll-like receptor (TLR) knockout mice	890:927	Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process was also evaluated.
34791999	9	64	theme	cell	1193:1196	arg1	subset					1198:1203	CD5+IgM+CD93-IL-10+B cell subset	1172:1203	CD5+IgM+CD93-IL-10+B cell subset	1172:1203	Under P. gingivalis LPS stimulation, CD5+IgM+CD93-IL-10+B cell subset increased significantly, which was regulated through TLR2/ 4.
34791999	4	65	theme	periodontitis	658:670	arg1	development					643:653	development	643:653	development	643:653	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	4	65	theme	periodontitis	658:670	arg1	occurrence					628:637	occurrence	628:637	occurrence	628:637	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	1	66	from	present	192:198	arg1	plaques					215:221	subgingival plaques	203:221	subgingival plaques of patients with periodontitis	203:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	67	theme	pathogenic	164:173	arg1	gingivalis					132:141	BACKGROUND Porphyromonas gingivalis	107:141	BACKGROUND Porphyromonas gingivalis (P. gingivalis)	107:157	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	67	theme	pathogenic	164:173	arg1	bacterium					175:183	a pathogenic bacterium	162:183	a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis	162:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	6	68	theme	TLR4	951:954	arg1	role					934:937	the role	930:937	the role of TLR2 and TLR4 in this process	930:970	Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process was also evaluated.
34791999	6	69	theme	TLR2	942:945	arg1	role					934:937	the role	930:937	the role of TLR2 and TLR4 in this process	930:970	Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in this process was also evaluated.
34791999	4	70	dep	gingivalis	556:565	arg1	LPS					587:589	LPS	587:589	LPS	587:589	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	4	70	dep	gingivalis	556:565	arg1	lipopolysaccharide					567:584	lipopolysaccharide	567:584	P. gingivalis lipopolysaccharide (LPS)	553:590	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	10	71	theme	gingivalis	1421:1430	arg1	effect					1408:1413	the effect	1404:1413	the effect of P. gingivalis on the production of Breg	1404:1456	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	5	72	theme	mRNA	697:700	arg1	levels					702:707	the mRNA levels	693:707	the mRNA levels of IL-10 in B cells	693:727	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	4	73	theme	P.	553:554	arg1	gingivalis					556:565	P. gingivalis lipopolysaccharide (LPS)	553:590	P. gingivalis lipopolysaccharide (LPS)	553:590	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	0	74	theme	B	52:52	arg1	Differentiation					59:73	B Cell Differentiation	52:73	B Cell Differentiation	52:73	Porphyromonas gingivalis Lipopolysaccharide-Induced B Cell Differentiation by Toll-like Receptors 2 and 4.
34791999	1	75	with	patients	226:233	arg1	periodontitis					240:252	periodontitis	240:252	periodontitis	240:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	76	theme	Porphyromonas	118:130	arg1	gingivalis					147:156	P. gingivalis	144:156	P. gingivalis	144:156	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	76	theme	Porphyromonas	118:130	arg1	bacterium					175:183	a pathogenic bacterium	162:183	a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis	162:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	76	theme	Porphyromonas	118:130	arg1	gingivalis					132:141	BACKGROUND Porphyromonas gingivalis	107:141	BACKGROUND Porphyromonas gingivalis (P. gingivalis)	107:157	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	2	77	theme	host	438:441	arg1	response					422:429	the natural immune response	403:429	the natural immune response of the host	403:441	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	1	78	attach	present	192:198	arg2	bacterium					175:183	a pathogenic bacterium	162:183	a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis	162:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	78	attach	present	192:198	arg2	gingivalis					132:141	BACKGROUND Porphyromonas gingivalis	107:141	BACKGROUND Porphyromonas gingivalis (P. gingivalis)	107:157	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	1	78	attach	present	192:198	arg1	plaques					215:221	subgingival plaques	203:221	subgingival plaques of patients with periodontitis	203:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	4	79	from	effect	543:548	arg1	Breg					615:618	Breg	615:618	Breg	615:618	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	4	79	from	effect	543:548	arg1	cells					608:612	regulatory B cells	595:612	regulatory B cells (Breg) in the occurrence and development of periodontitis	595:670	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	1	80	theme	patients	226:233	arg1	plaques					215:221	subgingival plaques	203:221	subgingival plaques of patients with periodontitis	203:252	BACKGROUND Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely present in subgingival plaques of patients with periodontitis.
34791999	2	81	theme	immune	415:420	arg1	response					422:429	the natural immune response	403:429	the natural immune response of the host	403:441	It induces periodontitis with bone loss as its main feature by changing the number and composition of symbiotic microorganisms, as well as inducing the natural immune response of the host.
34791999	5	82	theme	gingivalis	757:766	arg1	LPS					768:770	P. gingivalis LPS	754:770	P. gingivalis LPS	754:770	METHODS We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using flow cytometry.
34791999	4	83	dep	OBJECTIVE	498:506	arg1	aims					519:522	aims	519:522	aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis	519:670	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	10	84	from	investigations	1511:1524	arg1	development					1548:1558	development	1548:1558	development	1548:1558	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	10	84	from	investigations	1511:1524	arg1	occurrence					1533:1542	occurrence	1533:1542	occurrence	1533:1542	CONCLUSION The results of this study provides new insights into the immunopathogenic mechanism of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg, and present a theoretical foundation for subsequent investigations on the occurrence and development of periodontitis.
34791999	4	85	theme	B	606:606	arg1	Breg					615:618	Breg	615:618	Breg	615:618	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
34791999	4	85	theme	B	606:606	arg1	cells					608:612	regulatory B cells	595:612	regulatory B cells (Breg) in the occurrence and development of periodontitis	595:670	OBJECTIVE This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on regulatory B cells (Breg) in the occurrence and development of periodontitis.
37009158	8	0	used	used	1085:1088	arg2	staining					1072:1079	Hematoxylin and eosin (HE) staining	1045:1079	Hematoxylin and eosin (HE) staining	1045:1079	Hematoxylin and eosin (HE) staining was used to examine intestinal tissue morphology.
37009158	1	1	theme	metabolic	275:283	arg1	disorders					322:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	8	2	theme	intestinal	1101:1110	arg1	morphology					1119:1128	intestinal tissue morphology	1101:1128	intestinal tissue morphology	1101:1128	Hematoxylin and eosin (HE) staining was used to examine intestinal tissue morphology.
37009158	9	3	theme	expression	1135:1144	arg1	levels					1146:1151	The expression levels	1131:1151	The expression levels of tight junction (TJ) proteins and inflammatory cytokines	1131:1210	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	14	4	theme	ZO-1	1674:1677	arg1	expression					1679:1688	occludin and ZO-1 expression	1661:1688	occludin and ZO-1 expression	1661:1688	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	5	5	used	used	829:832	arg2	sequencing					814:823	16S rRNA sequencing	805:823	16S rRNA sequencing	805:823	16S rRNA sequencing was used to investigate gut microbiome changes.
37009158	1	6	theme	gut-brain	197:205	arg1	axis					207:210	the gut-brain axis	193:210	the gut-brain axis	193:210	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	6	theme	gut-brain	197:205	arg1	players					220:226	key players	216:226	key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders	216:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	6	theme	gut-brain	197:205	arg1	homeostasis					177:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	6	7	theme	open	877:880	arg1	test					888:891	The open field test	873:891	The open field test (OFT)	873:897	The open field test (OFT) and Y-maze were performed to evaluate brain function.
37009158	6	7	theme	open	877:880	arg1	OFT					894:896	OFT	894:896	OFT	894:896	The open field test (OFT) and Y-maze were performed to evaluate brain function.
37009158	1	8	from	alterations	260:270	arg1	disorders					322:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	4	9	theme	administered	692:703	arg1	SCFAs					705:709	administered SCFAs	692:709	administered SCFAs	692:709	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	9	10	theme	junction	1162:1169	arg1	proteins					1176:1183	tight junction (TJ) proteins	1156:1183	tight junction (TJ) proteins	1156:1183	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	15	11	theme	key	1876:1878	arg1	roles					1880:1884	key roles	1876:1884	key roles	1876:1884	Conclusions These findings suggested that disturbances in the gut microbiota and SCFA metabolites play key roles in SAE.
37009158	0	12	theme	sepsis-associated	110:126	arg1	encephalopathy					128:141	sepsis-associated encephalopathy	110:141	sepsis-associated encephalopathy	110:141	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	3	13	theme	gut	592:594	arg1	microbiome					596:605	gut microbiome	592:605	gut microbiome	592:605	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	6	14	theme	brain	937:941	arg1	function					943:950	brain function	937:950	brain function	937:950	The open field test (OFT) and Y-maze were performed to evaluate brain function.
37009158	11	15	used	used	1380:1383	arg2	Immunofluorescence					1357:1374	Immunofluorescence	1357:1374	Immunofluorescence	1357:1374	Immunofluorescence was used to examine the expression of TJ proteins.
37009158	4	16	theme	Male	669:672	arg1	mice					682:685	Male C57BL/6 mice	669:685	Male C57BL/6 mice	669:685	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	13	17	from	neuroinflammation	1612:1628	arg1	mice					1637:1640	SAE mice	1633:1640	SAE mice	1633:1640	SCFA treatment significantly alleviated behavioral dysfunction and neuroinflammation in SAE mice.
37009158	9	18	theme	proteins	1176:1183	arg1	levels					1146:1151	The expression levels	1131:1151	The expression levels of tight junction (TJ) proteins and inflammatory cytokines	1131:1210	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	5	19	theme	gut	849:851	arg1	changes					864:870	gut microbiome changes	849:870	gut microbiome changes	849:870	16S rRNA sequencing was used to investigate gut microbiome changes.
37009158	7	20	theme	Evans	1019:1023	arg1	staining					1035:1042	Evans blue (EB) staining	1019:1042	Evans blue (EB) staining	1019:1042	The permeability of the blood-brain barrier (BBB) was assessed by Evans blue (EB) staining.
37009158	9	21	theme	inflammatory	1189:1200	arg1	cytokines					1202:1210	inflammatory cytokines	1189:1210	inflammatory cytokines	1189:1210	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	3	22	theme	fatty	623:627	arg1	acid					629:632	short-chain fatty acid	611:632	short-chain fatty acid (SCFA)	611:639	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	3	22	theme	fatty	623:627	arg1	SCFA					635:638	SCFA	635:638	SCFA	635:638	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	7	23	theme	barrier	989:995	arg1	permeability					957:968	The permeability	953:968	The permeability of the blood-brain barrier (BBB)	953:1001	The permeability of the blood-brain barrier (BBB) was assessed by Evans blue (EB) staining.
37009158	1	24	from	health	249:254	arg1	disorders					322:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	25	theme	Background	144:153	arg1	axis					207:210	the gut-brain axis	193:210	the gut-brain axis	193:210	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	25	theme	Background	144:153	arg1	players					220:226	key players	216:226	key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders	216:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	25	theme	Background	144:153	arg1	homeostasis					177:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	12	26	theme	SAE	1488:1490	arg1	mice					1492:1495	SAE mice	1488:1495	SAE mice	1488:1495	Results The composition of the gut microbiota was altered in SAE mice; this change may be related to SCFA metabolism.
37009158	2	27	theme	life	532:535	arg1	quality					521:527	patient quality	513:527	patient quality of life	513:535	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	16	28	theme	BBB	1977:1979	arg1	integrity					1981:1989	BBB integrity	1977:1989	BBB integrity	1977:1989	SCFA supplementation could exert neuroprotective effects against SAE by preserving BBB integrity.
37009158	14	29	from	intestine	1697:1705	arg1	cells					1766:1770	LPS-treated cerebromicrovascular cells	1733:1770	LPS-treated cerebromicrovascular cells	1733:1770	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	14	29	from	intestine	1697:1705	arg1	mice					1724:1727	SAE mice	1720:1727	SAE mice	1720:1727	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	1	30	theme	microbiota	166:175	arg1	axis					207:210	the gut-brain axis	193:210	the gut-brain axis	193:210	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	30	theme	microbiota	166:175	arg1	players					220:226	key players	216:226	key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders	216:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	30	theme	microbiota	166:175	arg1	homeostasis					177:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	0	31	from	effects	15:21	arg1	brain					92:96	brain	92:96	brain	92:96	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	0	31	from	effects	15:21	arg1	integrity					78:86	blood-brain barrier integrity	58:86	blood-brain barrier integrity	58:86	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	0	32	theme	barrier	70:76	arg1	integrity					78:86	blood-brain barrier integrity	58:86	blood-brain barrier integrity	58:86	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	12	33	theme	microbiota	1462:1471	arg1	composition					1439:1449	The composition	1435:1449	The composition of the gut microbiota	1435:1471	Results The composition of the gut microbiota was altered in SAE mice; this change may be related to SCFA metabolism.
37009158	1	34	theme	inflammatory	286:297	arg1	disorders					322:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	2	35	theme	urgent	478:483	arg1	problem					495:501	an urgent, unsolved problem	475:501	problem	495:501	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	4	36	theme	ligation	754:761	arg1	surgery					782:788	cecal ligation and puncture (CLP) surgery	748:788	cecal ligation and puncture (CLP) surgery	748:788	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	5	37	theme	rRNA	809:812	arg1	sequencing					814:823	16S rRNA sequencing	805:823	16S rRNA sequencing	805:823	16S rRNA sequencing was used to investigate gut microbiome changes.
37009158	4	38	dep	subjected	735:743	arg1	SCFAs					705:709	administered SCFAs	692:709	administered SCFAs	692:709	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	13	39	theme	behavioral	1585:1594	arg1	dysfunction					1596:1606	behavioral dysfunction	1585:1606	behavioral dysfunction	1585:1606	SCFA treatment significantly alleviated behavioral dysfunction and neuroinflammation in SAE mice.
37009158	4	40	theme	puncture	767:774	arg1	surgery					782:788	cecal ligation and puncture (CLP) surgery	748:788	cecal ligation and puncture (CLP) surgery	748:788	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	11	41	theme	TJ	1414:1415	arg1	proteins					1417:1424	TJ proteins	1414:1424	TJ proteins	1414:1424	Immunofluorescence was used to examine the expression of TJ proteins.
37009158	2	42	theme	organ	453:457	arg1	dysfunction					459:469	a common secondary organ dysfunction	434:469	a common secondary organ dysfunction	434:469	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	2	42	theme	organ	453:457	arg1	encephalopathy					351:364	Sepsis-associated encephalopathy	333:364	Sepsis-associated encephalopathy (SAE)	333:370	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	1	43	theme	neurodegenerative	304:320	arg1	disorders					322:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	metabolic, inflammatory, and neurodegenerative disorders	275:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	0	44	theme	gut	26:28	arg1	microbes					30:37	gut microbes	26:37	gut microbes	26:37	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	14	45	theme	LPS-treated	1733:1743	arg1	cells					1766:1770	LPS-treated cerebromicrovascular cells	1733:1770	LPS-treated cerebromicrovascular cells	1733:1770	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	8	46	theme	Hematoxylin	1045:1055	arg1	staining					1072:1079	Hematoxylin and eosin (HE) staining	1045:1079	Hematoxylin and eosin (HE) staining	1045:1079	Hematoxylin and eosin (HE) staining was used to examine intestinal tissue morphology.
37009158	12	47	theme	gut	1458:1460	arg1	microbiota					1462:1471	the gut microbiota	1454:1471	the gut microbiota	1454:1471	Results The composition of the gut microbiota was altered in SAE mice; this change may be related to SCFA metabolism.
37009158	4	48	dep	Methods	661:667	arg1	subjected					735:743	subjected	735:743	subjected to cecal ligation and puncture (CLP) surgery to induce SAE	735:802	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	2	49	theme	unsolved	486:493	arg1	problem					495:501	an urgent, unsolved problem	475:501	problem	495:501	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	13	50	theme	SCFA	1545:1548	arg1	treatment					1550:1558	SCFA treatment	1545:1558	SCFA treatment	1545:1558	SCFA treatment significantly alleviated behavioral dysfunction and neuroinflammation in SAE mice.
37009158	8	51	theme	eosin	1061:1065	arg1	staining					1072:1079	Hematoxylin and eosin (HE) staining	1045:1079	Hematoxylin and eosin (HE) staining	1045:1079	Hematoxylin and eosin (HE) staining was used to examine intestinal tissue morphology.
37009158	16	52	theme	SCFA	1894:1897	arg1	supplementation					1899:1913	SCFA supplementation	1894:1913	SCFA supplementation	1894:1913	SCFA supplementation could exert neuroprotective effects against SAE by preserving BBB integrity.
37009158	1	53	theme	host	244:247	arg1	health					249:254	host health	244:254	host health	244:254	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	3	54	theme	neuroprotective	561:575	arg1	effects					577:583	the neuroprotective effects	557:583	the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE	557:658	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	13	55	from	dysfunction	1596:1606	arg1	mice					1637:1640	SAE mice	1633:1640	SAE mice	1633:1640	SCFA treatment significantly alleviated behavioral dysfunction and neuroinflammation in SAE mice.
37009158	16	56	theme	neuroprotective	1927:1941	arg1	effects					1943:1949	neuroprotective effects	1927:1949	neuroprotective effects	1927:1949	SCFA supplementation could exert neuroprotective effects against SAE by preserving BBB integrity.
37009158	5	57	theme	microbiome	853:862	arg1	changes					864:870	gut microbiome changes	849:870	gut microbiome changes	849:870	16S rRNA sequencing was used to investigate gut microbiome changes.
37009158	8	58	theme	tissue	1112:1117	arg1	morphology					1119:1128	intestinal tissue morphology	1101:1128	intestinal tissue morphology	1101:1128	Hematoxylin and eosin (HE) staining was used to examine intestinal tissue morphology.
37009158	6	59	theme	field	882:886	arg1	test					888:891	The open field test	873:891	The open field test (OFT)	873:897	The open field test (OFT) and Y-maze were performed to evaluate brain function.
37009158	6	59	theme	field	882:886	arg1	OFT					894:896	OFT	894:896	OFT	894:896	The open field test (OFT) and Y-maze were performed to evaluate brain function.
37009158	4	60	theme	C57BL/6	674:680	arg1	mice					682:685	Male C57BL/6 mice	669:685	Male C57BL/6 mice	669:685	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	14	61	from	brain	1711:1715	arg1	cells					1766:1770	LPS-treated cerebromicrovascular cells	1733:1770	LPS-treated cerebromicrovascular cells	1733:1770	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	14	61	from	brain	1711:1715	arg1	mice					1724:1727	SAE mice	1720:1727	SAE mice	1720:1727	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	9	62	theme	tight	1156:1160	arg1	TJ					1172:1173	TJ	1172:1173	TJ	1172:1173	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	9	62	theme	tight	1156:1160	arg1	junction					1162:1169	tight junction	1156:1169	tight junction (TJ) proteins	1156:1183	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	3	63	theme	microbiome	596:605	arg1	metabolites					641:651	the gut microbiome and short-chain fatty acid (SCFA) metabolites	588:651	the gut microbiome and short-chain fatty acid (SCFA) metabolites	588:651	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	12	64	theme	SCFA	1528:1531	arg1	metabolism					1533:1542	SCFA metabolism	1528:1542	SCFA metabolism	1528:1542	Results The composition of the gut microbiota was altered in SAE mice; this change may be related to SCFA metabolism.
37009158	7	65	theme	EB	1031:1032	arg1	staining					1035:1042	Evans blue (EB) staining	1019:1042	Evans blue (EB) staining	1019:1042	The permeability of the blood-brain barrier (BBB) was assessed by Evans blue (EB) staining.
37009158	2	66	theme	Sepsis-associated	333:349	arg1	SAE					367:369	SAE	367:369	SAE	367:369	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	2	66	theme	Sepsis-associated	333:349	arg1	encephalopathy					351:364	Sepsis-associated encephalopathy	333:364	Sepsis-associated encephalopathy (SAE)	333:370	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	2	66	theme	Sepsis-associated	333:349	arg1	dysfunction					459:469	a common secondary organ dysfunction	434:469	a common secondary organ dysfunction	434:469	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	1	67	theme	key	216:218	arg1	axis					207:210	the gut-brain axis	193:210	the gut-brain axis	193:210	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	67	theme	key	216:218	arg1	players					220:226	key players	216:226	key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders	216:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	67	theme	key	216:218	arg1	homeostasis					177:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	15	68	theme	SCFA	1854:1857	arg1	metabolites					1859:1869	the gut microbiota and SCFA metabolites	1831:1869	metabolites	1859:1869	Conclusions These findings suggested that disturbances in the gut microbiota and SCFA metabolites play key roles in SAE.
37009158	7	69	theme	blue	1025:1028	arg1	staining					1035:1042	Evans blue (EB) staining	1019:1042	Evans blue (EB) staining	1019:1042	The permeability of the blood-brain barrier (BBB) was assessed by Evans blue (EB) staining.
37009158	14	70	theme	occludin	1661:1668	arg1	expression					1679:1688	occludin and ZO-1 expression	1661:1688	occludin and ZO-1 expression	1661:1688	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	3	71	theme	acid	629:632	arg1	metabolites					641:651	the gut microbiome and short-chain fatty acid (SCFA) metabolites	588:651	the gut microbiome and short-chain fatty acid (SCFA) metabolites	588:651	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	3	72	theme	short-chain	611:621	arg1	acid					629:632	short-chain fatty acid	611:632	short-chain fatty acid (SCFA)	611:639	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	3	72	theme	short-chain	611:621	arg1	SCFA					635:638	SCFA	635:638	SCFA	635:638	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	9	73	theme	cytokines	1202:1210	arg1	levels					1146:1151	The expression levels	1131:1151	The expression levels of tight junction (TJ) proteins and inflammatory cytokines	1131:1210	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	3	74	theme	metabolites	641:651	arg1	effects					577:583	the neuroprotective effects	557:583	the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE	557:658	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	15	75	dep	Conclusions	1773:1783	arg1	suggested					1800:1808	suggested	1800:1808	suggested that disturbances in the gut microbiota and SCFA metabolites play key roles in SAE	1800:1891	Conclusions These findings suggested that disturbances in the gut microbiota and SCFA metabolites play key roles in SAE.
37009158	11	76	theme	proteins	1417:1424	arg1	expression					1400:1409	the expression	1396:1409	the expression of TJ proteins	1396:1424	Immunofluorescence was used to examine the expression of TJ proteins.
37009158	12	77	dep	Results	1427:1433	arg1	altered					1477:1483	altered	1477:1483	was altered in SAE mice	1473:1495	Results The composition of the gut microbiota was altered in SAE mice; this change may be related to SCFA metabolism.
37009158	0	78	theme	blood-brain	58:68	arg1	integrity					78:86	blood-brain barrier integrity	58:86	blood-brain barrier integrity	58:86	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	7	79	theme	blood-brain	977:987	arg1	BBB					998:1000	BBB	998:1000	BBB	998:1000	The permeability of the blood-brain barrier (BBB) was assessed by Evans blue (EB) staining.
37009158	7	79	theme	blood-brain	977:987	arg1	barrier					989:995	the blood-brain barrier	973:995	the blood-brain barrier (BBB)	973:1001	The permeability of the blood-brain barrier (BBB) was assessed by Evans blue (EB) staining.
37009158	1	80	theme	Intestinal	155:164	arg1	axis					207:210	the gut-brain axis	193:210	the gut-brain axis	193:210	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	80	theme	Intestinal	155:164	arg1	players					220:226	key players	216:226	key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders	216:330	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	1	80	theme	Intestinal	155:164	arg1	homeostasis					177:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis	144:187	Background Intestinal microbiota homeostasis and the gut-brain axis are key players associated with host health and alterations in metabolic, inflammatory, and neurodegenerative disorders.
37009158	9	81	theme	western	1228:1234	arg1	blots					1236:1240	western blots	1228:1240	western blots	1228:1240	The expression levels of tight junction (TJ) proteins and inflammatory cytokines was assessed by western blots and immunohistochemistry.
37009158	2	82	theme	patient	513:519	arg1	quality					521:527	patient quality	513:527	patient quality of life	513:535	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	3	83	from	effects	577:583	arg1	SAE					656:658	SAE	656:658	SAE	656:658	Our study examined the neuroprotective effects of the gut microbiome and short-chain fatty acid (SCFA) metabolites on SAE.
37009158	14	84	dep	intestine	1697:1705	arg1	the					1693:1695	the	1693:1695	the	1693:1695	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	13	85	theme	SAE	1633:1635	arg1	mice					1637:1640	SAE mice	1633:1640	SAE mice	1633:1640	SCFA treatment significantly alleviated behavioral dysfunction and neuroinflammation in SAE mice.
37009158	5	86	theme	16S	805:807	arg1	sequencing					814:823	16S rRNA sequencing	805:823	16S rRNA sequencing	805:823	16S rRNA sequencing was used to investigate gut microbiome changes.
37009158	4	87	theme	cecal	748:752	arg1	ligation					754:761	cecal ligation	748:761	cecal ligation	748:761	Methods Male C57BL/6 mice were administered SCFAs in drinking water, then subjected to cecal ligation and puncture (CLP) surgery to induce SAE.
37009158	2	88	theme	bacterial	406:414	arg1	translocation					416:428	bacterial translocation	406:428	bacterial translocation	406:428	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	2	89	theme	secondary	443:451	arg1	dysfunction					459:469	a common secondary organ dysfunction	434:469	a common secondary organ dysfunction	434:469	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	2	89	theme	secondary	443:451	arg1	encephalopathy					351:364	Sepsis-associated encephalopathy	333:364	Sepsis-associated encephalopathy (SAE)	333:370	Sepsis-associated encephalopathy (SAE), which is closely associated with bacterial translocation, is a common secondary organ dysfunction and an urgent, unsolved problem affecting patient quality of life.
37009158	0	90	theme	microbes	30:37	arg1	effects					15:21	The modulatory effects	0:21	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain	0:96	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	14	91	theme	cerebromicrovascular	1745:1764	arg1	cells					1766:1770	LPS-treated cerebromicrovascular cells	1733:1770	LPS-treated cerebromicrovascular cells	1733:1770	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	0	92	theme	metabolites	43:53	arg1	effects					15:21	The modulatory effects	0:21	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain	0:96	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
37009158	15	93	from	disturbances	1815:1826	arg1	microbiota					1839:1848	the gut microbiota and SCFA metabolites	1831:1869	microbiota	1839:1848	Conclusions These findings suggested that disturbances in the gut microbiota and SCFA metabolites play key roles in SAE.
37009158	15	93	from	disturbances	1815:1826	arg1	metabolites					1859:1869	the gut microbiota and SCFA metabolites	1831:1869	metabolites	1859:1869	Conclusions These findings suggested that disturbances in the gut microbiota and SCFA metabolites play key roles in SAE.
37009158	10	94	theme	bEND.3	1278:1283	arg1	cells					1285:1289	bEND.3 cells	1278:1289	bEND.3 cells	1278:1289	In vitro, bEND.3 cells were incubated with SCFAs and then with lipopolysaccharide (LPS).
37009158	14	95	theme	SAE	1720:1722	arg1	mice					1724:1727	SAE mice	1720:1727	SAE mice	1720:1727	SCFAs upregulated occludin and ZO-1 expression in the intestine and brain in SAE mice and LPS-treated cerebromicrovascular cells.
37009158	15	96	theme	gut	1835:1837	arg1	microbiota					1839:1848	the gut microbiota and SCFA metabolites	1831:1869	microbiota	1839:1848	Conclusions These findings suggested that disturbances in the gut microbiota and SCFA metabolites play key roles in SAE.
37009158	0	97	theme	modulatory	4:13	arg1	effects					15:21	The modulatory effects	0:21	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain	0:96	The modulatory effects of gut microbes and metabolites on blood-brain barrier integrity and brain function in sepsis-associated encephalopathy.
35836813	10	0	from	detrimental	1962:1972	arg1	face					1981:1984	the face	1977:1984	the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD	1977:2281	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	5	1	theme	colitis	929:935	arg1	development					914:924	the development	910:924	the development of colitis in IL-37tg mice	910:951	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	2	2	theme	factors	373:379	arg1	contribution					343:354	the contribution	339:354	the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations	339:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	10	3	theme	intestinal	2189:2198	arg1	homeostasis					2221:2231	intestinal microbial and immune homeostasis	2189:2231	intestinal microbial and immune homeostasis	2189:2231	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	9	4	theme	IL-37-caused	1625:1636	arg1	inflammation					1644:1655	IL-37-caused colon inflammation	1625:1655	IL-37-caused colon inflammation	1625:1655	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	6	5	theme	chemokines	1219:1228	arg1	expression					1183:1192	the expression	1179:1192	the expression of diverse cytokines and chemokines	1179:1228	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	5	theme	chemokines	1219:1228	arg1	function					1143:1150	intestinal epithelial barrier function	1113:1150	intestinal epithelial barrier function	1113:1150	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	8	6	from	mice	1458:1461	arg1	results					1429:1435	These conflicting results	1411:1435	These conflicting results from IL-37tg colitis mice	1411:1461	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	5	7	theme	microbiota	896:905	arg1	efficacy					884:891	The efficacy	880:891	The efficacy of microbiota in the development of colitis in IL-37tg mice	880:951	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	6	8	theme	cytokines	1205:1213	arg1	expression					1183:1192	the expression	1179:1192	the expression of diverse cytokines and chemokines	1179:1228	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	8	theme	cytokines	1205:1213	arg1	function					1143:1150	intestinal epithelial barrier function	1113:1150	intestinal epithelial barrier function	1113:1150	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	3	9	theme	human	628:632	arg1	IL-37tg					641:647	IL-37tg	641:647	IL-37tg	641:647	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	3	9	theme	human	628:632	arg1	IL-37					634:638	human IL-37	628:638	human IL-37 (IL-37tg)	628:648	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	5	10	theme	antibiotic	976:985	arg1	treatment					987:995	antibiotic treatment	976:995	antibiotic treatment	976:995	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	10	11	theme	exacerbated	2050:2060	arg1	occurrences					2066:2076	exacerbated IBD occurrences	2050:2076	exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD	2050:2281	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	12	theme	promising	2244:2252	arg1	strategy					2266:2273	a promising therapeutic strategy	2242:2273	a promising therapeutic strategy for IBD	2242:2281	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	9	13	theme	lamina	1799:1804	arg1	propria					1806:1812	colon lamina propria	1793:1812	colon lamina propria	1793:1812	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	8	14	theme	microbiota	1502:1511	arg1	dysbiosis					1481:1489	a dysbiosis	1479:1489	a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice	1479:1580	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	3	15	theme	colitis	790:796	arg1	model					744:748	a mouse model	736:748	a mouse model of dextran sulfate sodium (DSS)-induced colitis	736:796	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	6	16	theme	signaling	1252:1260	arg1	pathways					1262:1269	activated signaling pathways	1242:1269	intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways	1113:1269	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	3	17	theme	conventional	668:679	arg1	conditions					714:723	conventional and specific pathogen-free (SPF) conditions	668:723	conventional and specific pathogen-free (SPF) conditions	668:723	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	3	17	theme	conventional	668:679	arg1	SPF					709:711	SPF	709:711	SPF	709:711	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	1	18	theme	complicated	149:159	arg1	crosstalk					161:169	complicated crosstalk	149:169	complicated crosstalk between host immunity and the gut microbiome	149:214	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	9	19	theme	lymph	1829:1833	arg1	node					1835:1838	mesenteric lymph node	1818:1838	mesenteric lymph node	1818:1838	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	7	20	theme	SPF	1401:1403	arg1	mice					1405:1408	SPF mice	1401:1408	SPF mice	1401:1408	Results: We found that IL-37 overexpression aggravated DSS-induced colitis in conventional mice but protected against colitis in SPF mice.
35836813	10	21	theme	immune	2109:2114	arg1	system					2116:2121	the immune system	2105:2121	the immune system	2105:2121	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	2	22	theme	pathogenic	521:530	arg1	functions					547:555	pathogenic and protective functions	521:555	pathogenic and protective functions related to microbiota alterations	521:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	10	23	theme	gut	2150:2152	arg1	bacteria					2165:2172	gut pathogenic bacteria	2150:2172	gut pathogenic bacteria	2150:2172	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	2	24	theme	microbiota	568:577	arg1	alterations					579:589	microbiota alterations	568:589	microbiota alterations	568:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	8	25	theme	conventional	1564:1575	arg1	mice					1577:1580	IL-37tg conventional mice	1556:1580	IL-37tg conventional mice	1556:1580	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	5	26	from	development	914:924	arg1	mice					948:951	IL-37tg mice	940:951	IL-37tg mice	940:951	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	5	27	theme	microbiota	1007:1016	arg1	FMT					1035:1037	FMT	1035:1037	FMT	1035:1037	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	5	27	theme	microbiota	1007:1016	arg1	transplantation					1018:1032	fecal microbiota transplantation	1001:1032	fecal microbiota transplantation (FMT)	1001:1038	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	9	28	from	recruitment	1778:1788	arg1	propria					1806:1812	colon lamina propria	1793:1812	colon lamina propria	1793:1812	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	9	28	from	recruitment	1778:1788	arg1	node					1835:1838	mesenteric lymph node	1818:1838	mesenteric lymph node	1818:1838	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	2	29	theme	related	557:563	arg1	functions					547:555	pathogenic and protective functions	521:555	pathogenic and protective functions related to microbiota alterations	521:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	4	30	theme	ribosomal	803:811	arg1	sequencing					817:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing was used for analyzing fecal microbial communities.
35836813	2	31	theme	colitis	422:428	arg1	mice					430:433	colitis mice	422:433	colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations	422:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	6	32	theme	intestinal	1113:1122	arg1	infiltration					1165:1176	immune cell infiltration	1153:1176	immune cell infiltration	1153:1176	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	32	theme	intestinal	1113:1122	arg1	expression					1183:1192	the expression	1179:1192	the expression of diverse cytokines and chemokines	1179:1228	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	32	theme	intestinal	1113:1122	arg1	function					1143:1150	intestinal epithelial barrier function	1113:1150	intestinal epithelial barrier function	1113:1150	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	9	33	theme	conventional	1885:1896	arg1	mice					1898:1901	IL-37tg conventional mice	1877:1901	IL-37tg conventional mice	1877:1901	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	2	34	theme	protective	536:545	arg1	functions					547:555	pathogenic and protective functions	521:555	pathogenic and protective functions related to microbiota alterations	521:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	1	35	theme	bowel	120:124	arg1	IBD					135:137	IBD	135:137	IBD	135:137	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	1	35	theme	bowel	120:124	arg1	disease					126:132	Inflammatory bowel disease	107:132	Inflammatory bowel disease (IBD)	107:138	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	2	36	theme	human	460:464	arg1	IL-37					466:470	human IL-37	460:470	human IL-37	460:470	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	2	36	theme	human	460:464	arg1	cytokine					501:508	a natural anti-inflammatory cytokine	473:508	a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations	473:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	8	37	theme	IL-37tg	1442:1448	arg1	mice					1458:1461	IL-37tg colitis mice	1442:1461	IL-37tg colitis mice	1442:1461	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	0	38	theme	experimental	27:38	arg1	colitis					40:46	experimental colitis	27:46	experimental colitis	27:46	Interleukin-37 exacerbates experimental colitis in an intestinal microbiome-dependent fashion.
35836813	7	39	theme	conventional	1350:1361	arg1	mice					1363:1366	conventional mice	1350:1366	conventional mice	1350:1366	Results: We found that IL-37 overexpression aggravated DSS-induced colitis in conventional mice but protected against colitis in SPF mice.
35836813	2	40	theme	natural	475:481	arg1	IL-37					466:470	human IL-37	460:470	human IL-37	460:470	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	2	40	theme	natural	475:481	arg1	cytokine					501:508	a natural anti-inflammatory cytokine	473:508	a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations	473:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	3	41	theme	specific	685:692	arg1	conditions					714:723	conventional and specific pathogen-free (SPF) conditions	668:723	conventional and specific pathogen-free (SPF) conditions	668:723	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	3	41	theme	specific	685:692	arg1	SPF					709:711	SPF	709:711	SPF	709:711	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	4	42	theme	microbial	857:865	arg1	communities					867:877	fecal microbial communities	851:877	fecal microbial communities	851:877	16S ribosomal RNA sequencing was used for analyzing fecal microbial communities.
35836813	2	43	theme	environmental	359:371	arg1	factors					373:379	environmental factors	359:379	environmental factors	359:379	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	0	44	theme	intestinal	54:63	arg1	fashion					86:92	an intestinal microbiome-dependent fashion	51:92	an intestinal microbiome-dependent fashion	51:92	Interleukin-37 exacerbates experimental colitis in an intestinal microbiome-dependent fashion.
35836813	9	45	theme	intestinal	1680:1689	arg1	barrier					1702:1708	intestinal epithelial barrier	1680:1708	intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells	1680:1776	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	9	46	theme	colon	1638:1642	arg1	inflammation					1644:1655	IL-37-caused colon inflammation	1625:1655	IL-37-caused colon inflammation	1625:1655	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	9	47	theme	barrier	1702:1708	arg1	impairment					1710:1719	intestinal epithelial barrier impairment	1680:1719	intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells	1680:1776	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	6	48	theme	cell	1160:1163	arg1	infiltration					1165:1176	immune cell infiltration	1153:1176	immune cell infiltration	1153:1176	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	48	theme	cell	1160:1163	arg1	function					1143:1150	intestinal epithelial barrier function	1113:1150	intestinal epithelial barrier function	1113:1150	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	2	49	theme	gut	392:394	arg1	composition					407:417	shaping gut microbiota composition	384:417	shaping gut microbiota composition	384:417	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	7	50	theme	IL-37	1295:1299	arg1	overexpression					1301:1314	IL-37 overexpression	1295:1314	IL-37 overexpression	1295:1314	Results: We found that IL-37 overexpression aggravated DSS-induced colitis in conventional mice but protected against colitis in SPF mice.
35836813	10	51	theme	immune	2214:2219	arg1	homeostasis					2221:2231	intestinal microbial and immune homeostasis	2189:2231	intestinal microbial and immune homeostasis	2189:2231	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	52	theme	immunoregulatory	1921:1936	arg1	detrimental					1962:1972	detrimental	1962:1972	detrimental	1962:1972	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	52	theme	immunoregulatory	1921:1936	arg1	properties					1938:1947	The immunoregulatory properties	1917:1947	The immunoregulatory properties of IL-37	1917:1956	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	1	53	theme	intestinal	258:267	arg1	inflammation					269:280	intestinal inflammation	258:280	intestinal inflammation	258:280	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	9	54	theme	pathogenic	1731:1740	arg1	bacteria					1742:1749	pathogenic bacteria	1731:1749	pathogenic bacteria	1731:1749	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	2	55	from	contribution	343:354	arg1	mice					430:433	colitis mice	422:433	colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations	422:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	6	56	theme	barrier	1135:1141	arg1	infiltration					1165:1176	immune cell infiltration	1153:1176	immune cell infiltration	1153:1176	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	56	theme	barrier	1135:1141	arg1	expression					1183:1192	the expression	1179:1192	the expression of diverse cytokines and chemokines	1179:1228	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	56	theme	barrier	1135:1141	arg1	function					1143:1150	intestinal epithelial barrier function	1113:1150	intestinal epithelial barrier function	1113:1150	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	10	57	theme	microbial	2200:2208	arg1	homeostasis					2221:2231	intestinal microbial and immune homeostasis	2189:2231	intestinal microbial and immune homeostasis	2189:2231	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	58	theme	dysbiosis	1989:1997	arg1	face					1981:1984	the face	1977:1984	the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD	1977:2281	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	3	59	dep	expressing	617:626	arg1	transgenic					606:615	transgenic	606:615	transgenic	606:615	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	10	60	from	face	1981:1984	arg1	detrimental					1962:1972	detrimental	1962:1972	detrimental	1962:1972	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	60	from	face	1981:1984	arg1	Conclusions					1904:1914	Conclusions	1904:1914	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.	1904:2282	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	60	from	face	1981:1984	arg1	properties					1938:1947	The immunoregulatory properties	1917:1947	The immunoregulatory properties of IL-37	1917:1956	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	61	theme	microbiota	2017:2026	arg1	dysbiosis					1989:1997	dysbiosis	1989:1997	dysbiosis	1989:1997	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	1	62	theme	gut	201:203	arg1	microbiome					205:214	the gut microbiome	197:214	the gut microbiome	197:214	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	9	63	theme	NK	1769:1770	arg1	cells					1772:1776	NK cells	1769:1776	NK cells	1769:1776	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	6	64	theme	diverse	1197:1203	arg1	cytokines					1205:1213	diverse cytokines	1197:1213	diverse cytokines	1197:1213	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	3	65	theme	-induced	781:788	arg1	colitis					790:796	dextran sulfate sodium (DSS)-induced colitis	753:796	dextran sulfate sodium (DSS)-induced colitis	753:796	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	4	66	used	used	832:835	arg2	sequencing					817:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing was used for analyzing fecal microbial communities.
35836813	8	67	theme	gut	1498:1500	arg1	microbiota					1502:1511	the gut microbiota	1494:1511	the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice	1494:1580	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	10	68	theme	therapeutic	2254:2264	arg1	strategy					2266:2273	a promising therapeutic strategy	2242:2273	a promising therapeutic strategy for IBD	2242:2281	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	9	69	theme	colon	1793:1797	arg1	propria					1806:1812	colon lamina propria	1793:1812	colon lamina propria	1793:1812	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	9	70	theme	inflammatory	1851:1862	arg1	responses					1864:1872	inflammatory responses	1851:1872	inflammatory responses in IL-37tg conventional mice	1851:1901	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	10	71	theme	IBD	2062:2064	arg1	occurrences					2066:2076	exacerbated IBD occurrences	2050:2076	exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD	2050:2281	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	5	72	from	efficacy	884:891	arg1	development					914:924	the development	910:924	the development of colitis in IL-37tg mice	910:951	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	5	73	theme	IL-37tg	940:946	arg1	mice					948:951	IL-37tg mice	940:951	IL-37tg mice	940:951	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	8	74	theme	detrimental	1522:1532	arg1	bacteria					1534:1541	detrimental bacteria	1522:1541	detrimental bacteria	1522:1541	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	9	75	theme	mesenteric	1818:1827	arg1	node					1835:1838	mesenteric lymph node	1818:1838	mesenteric lymph node	1818:1838	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	6	76	theme	activated	1242:1250	arg1	pathways					1262:1269	activated signaling pathways	1242:1269	intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways	1113:1269	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	8	77	theme	IL-37tg	1556:1562	arg1	mice					1577:1580	IL-37tg conventional mice	1556:1580	IL-37tg conventional mice	1556:1580	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	1	78	theme	host	179:182	arg1	immunity					184:191	host immunity	179:191	host immunity	179:191	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	5	79	theme	fecal	1001:1005	arg1	FMT					1035:1037	FMT	1035:1037	FMT	1035:1037	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	5	79	theme	fecal	1001:1005	arg1	transplantation					1018:1032	fecal microbiota transplantation	1001:1032	fecal microbiota transplantation (FMT)	1001:1038	The efficacy of microbiota in the development of colitis in IL-37tg mice was investigated after antibiotic treatment and fecal microbiota transplantation (FMT).
35836813	6	80	theme	epithelial	1124:1133	arg1	infiltration					1165:1176	immune cell infiltration	1153:1176	immune cell infiltration	1153:1176	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	80	theme	epithelial	1124:1133	arg1	expression					1183:1192	the expression	1179:1192	the expression of diverse cytokines and chemokines	1179:1228	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	80	theme	epithelial	1124:1133	arg1	function					1143:1150	intestinal epithelial barrier function	1113:1150	intestinal epithelial barrier function	1113:1150	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	9	81	theme	IL-37tg	1877:1883	arg1	mice					1898:1901	IL-37tg conventional mice	1877:1901	IL-37tg conventional mice	1877:1901	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	4	82	theme	16S	799:801	arg1	sequencing					817:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing was used for analyzing fecal microbial communities.
35836813	1	83	theme	Inflammatory	107:118	arg1	IBD					135:137	IBD	135:137	IBD	135:137	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	1	83	theme	Inflammatory	107:118	arg1	disease					126:132	Inflammatory bowel disease	107:132	Inflammatory bowel disease (IBD)	107:138	Background: Inflammatory bowel disease (IBD) involves complicated crosstalk between host immunity and the gut microbiome, whereas the mechanics of how they govern intestinal inflammation remain poorly understood.
35836813	8	84	theme	conflicting	1417:1427	arg1	results					1429:1435	These conflicting results	1411:1435	These conflicting results from IL-37tg colitis mice	1411:1461	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	2	85	theme	microbiota	396:405	arg1	composition					407:417	shaping gut microbiota composition	384:417	shaping gut microbiota composition	384:417	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	9	86	theme	inflammation	1644:1655	arg1	outcome					1614:1620	the outcome	1610:1620	the outcome of IL-37-caused colon inflammation	1610:1655	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	9	86	theme	inflammation	1644:1655	arg1	related					1669:1675	related	1669:1675	related	1669:1675	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	3	87	theme	pathogen-free	694:706	arg1	conditions					714:723	conventional and specific pathogen-free (SPF) conditions	668:723	conventional and specific pathogen-free (SPF) conditions	668:723	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	3	87	theme	pathogen-free	694:706	arg1	SPF					709:711	SPF	709:711	SPF	709:711	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	2	88	theme	anti-inflammatory	483:499	arg1	IL-37					466:470	human IL-37	460:470	human IL-37	460:470	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	2	88	theme	anti-inflammatory	483:499	arg1	cytokine					501:508	a natural anti-inflammatory cytokine	473:508	a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations	473:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	10	89	theme	IL-37	1952:1956	arg1	detrimental					1962:1972	detrimental	1962:1972	detrimental	1962:1972	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	10	89	theme	IL-37	1952:1956	arg1	properties					1938:1947	The immunoregulatory properties	1917:1947	The immunoregulatory properties of IL-37	1917:1956	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	8	90	theme	colitis	1450:1456	arg1	mice					1458:1461	IL-37tg colitis mice	1442:1461	IL-37tg colitis mice	1442:1461	These conflicting results from IL-37tg colitis mice are ascribed to a dysbiosis of the gut microbiota in which detrimental bacteria increased in IL-37tg conventional mice.
35836813	2	91	contain	possessing	510:519	arg1	IL-37					466:470	human IL-37	460:470	human IL-37	460:470	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	2	91	contain	possessing	510:519	arg2	functions					547:555	pathogenic and protective functions	521:555	pathogenic and protective functions related to microbiota alterations	521:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	2	91	contain	possessing	510:519	arg1	cytokine					501:508	a natural anti-inflammatory cytokine	473:508	a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations	473:589	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35836813	0	92	theme	microbiome-dependent	65:84	arg1	fashion					86:92	an intestinal microbiome-dependent fashion	51:92	an intestinal microbiome-dependent fashion	51:92	Interleukin-37 exacerbates experimental colitis in an intestinal microbiome-dependent fashion.
35836813	9	93	from	responses	1864:1872	arg1	mice					1898:1901	IL-37tg conventional mice	1877:1901	IL-37tg conventional mice	1877:1901	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	7	94	theme	DSS-induced	1327:1337	arg1	colitis					1339:1345	DSS-induced colitis	1327:1345	DSS-induced colitis	1327:1345	Results: We found that IL-37 overexpression aggravated DSS-induced colitis in conventional mice but protected against colitis in SPF mice.
35836813	10	95	theme	intestinal	2006:2015	arg1	microbiota					2017:2026	the intestinal microbiota	2002:2026	the intestinal microbiota	2002:2026	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	4	96	theme	fecal	851:855	arg1	communities					867:877	fecal microbial communities	851:877	fecal microbial communities	851:877	16S ribosomal RNA sequencing was used for analyzing fecal microbial communities.
35836813	9	97	theme	epithelial	1691:1700	arg1	barrier					1702:1708	intestinal epithelial barrier	1680:1708	intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells	1680:1776	We further identified that the outcome of IL-37-caused colon inflammation is strongly related to intestinal epithelial barrier impairment caused by pathogenic bacteria, neutrophils, and NK cells recruitment in colon lamina propria and mesenteric lymph node to enhance inflammatory responses in IL-37tg conventional mice.
35836813	4	98	theme	RNA	813:815	arg1	sequencing					817:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing	799:826	16S ribosomal RNA sequencing was used for analyzing fecal microbial communities.
35836813	6	99	theme	immune	1153:1158	arg1	infiltration					1165:1176	immune cell infiltration	1153:1176	immune cell infiltration	1153:1176	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	6	99	theme	immune	1153:1158	arg1	function					1143:1150	intestinal epithelial barrier function	1113:1150	intestinal epithelial barrier function	1113:1150	The mechanism by which IL-37 worsened colitis was studied by evaluating intestinal epithelial barrier function, immune cell infiltration, the expression of diverse cytokines and chemokines, as well as activated signaling pathways.
35836813	10	100	theme	pathogenic	2154:2163	arg1	bacteria					2165:2172	gut pathogenic bacteria	2150:2172	gut pathogenic bacteria	2150:2172	Conclusions: The immunoregulatory properties of IL-37 are detrimental in the face of dysbiosis of the intestinal microbiota, which contributes to exacerbated IBD occurrences that are uncontrollable by the immune system, suggesting that depleting gut pathogenic bacteria or maintaining intestinal microbial and immune homeostasis could be a promising therapeutic strategy for IBD.
35836813	3	101	theme	mouse	738:742	arg1	model					744:748	a mouse model	736:748	a mouse model of dextran sulfate sodium (DSS)-induced colitis	736:796	Methods: Mice transgenic expressing human IL-37 (IL-37tg) were housed under conventional and specific pathogen-free (SPF) conditions to develop a mouse model of dextran sulfate sodium (DSS)-induced colitis.
35836813	2	102	theme	shaping	384:390	arg1	composition					407:417	shaping gut microbiota composition	384:417	shaping gut microbiota composition	384:417	In this study, we investigated the contribution of environmental factors to shaping gut microbiota composition in colitis mice that were transgenic for human IL-37, a natural anti-inflammatory cytokine possessing pathogenic and protective functions related to microbiota alterations.
35628640	0	0	theme	Rheumatoid	67:76	arg1	Arthritis					78:86	Rheumatoid Arthritis	67:86	Rheumatoid Arthritis	67:86	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.
35628640	3	1	theme	relative	299:306	arg1	level					340:344	the relative sialylation and galactosylation level	295:344	the relative sialylation and galactosylation level of ACPAs and control IgG	295:369	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	10	2	theme	terminal	1468:1475	arg1	moieties					1489:1496	terminal sialic acid moieties	1468:1496	terminal sialic acid moieties	1468:1496	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	3	3	theme	sialylation	308:318	arg1	level					340:344	the relative sialylation and galactosylation level	295:344	the relative sialylation and galactosylation level of ACPAs and control IgG	295:369	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	0	4	from	Role	4:7	arg1	Pathomechanism					49:62	the Pathomechanism	45:62	the Pathomechanism of Rheumatoid Arthritis	45:86	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.
35628640	9	5	theme	Functional	1211:1220	arg1	assays					1222:1227	Functional assays	1211:1227	Functional assays	1211:1227	Functional assays show that dimerized ACPA-IgG significantly enhances TNFα release in an FcγRI-dependent manner, whereas healthy IgG does not.
35628640	3	6	from	composition	484:494	arg1	RA					538:539	RA	538:539	RA	538:539	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	7	7	gly	glycopeptides	960:972	arg2	glycopeptides					960:972	glycopeptides	960:972	glycopeptides	960:972	To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS.
35628640	3	8	from	markers	527:533	arg1	RA					538:539	RA	538:539	RA	538:539	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	3	9	theme	ACPAs	349:353	arg1	level					340:344	the relative sialylation and galactosylation level	295:344	the relative sialylation and galactosylation level of ACPAs and control IgG	295:369	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	6	10	theme	TNFα	812:815	arg1	release					817:823	TNFα release	812:823	TNFα release determined by ELISA	812:843	U937 cells were used to monitor the binding of IC to FcγR and to trigger TNFα release determined by ELISA.
35628640	10	11	theme	relative	1400:1407	arg1	intensities					1409:1419	the relative intensities	1396:1419	the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties	1396:1496	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	7	12	theme	control	874:880	arg1	IgG					882:884	control IgG	874:884	control IgG	874:884	To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS.
35628640	3	13	theme	markers	527:533	arg1	composition					484:494	the composition	480:494	the composition of Fc glycans and inflammatory markers in RA	480:539	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	10	14	gly	glycoform	1431:1439	arg1	G0					1428:1429	the G0 glycoform	1424:1439	the G0 glycoform	1424:1439	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	0	15	theme	Arthritis	78:86	arg1	Pathomechanism					49:62	the Pathomechanism	45:62	the Pathomechanism of Rheumatoid Arthritis	45:86	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.
35628640	6	16	theme	U937	739:742	arg1	cells					744:748	U937 cells	739:748	U937 cells	739:748	U937 cells were used to monitor the binding of IC to FcγR and to trigger TNFα release determined by ELISA.
35628640	8	17	theme	ACPA-IgG	1118:1125	arg1	levels					1108:1113	both sialylation and galactosylation levels	1071:1113	both sialylation and galactosylation levels of ACPA-IgG	1071:1125	We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF.
35628640	3	18	from	glycans	502:508	arg1	RA					538:539	RA	538:539	RA	538:539	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	1	19	theme	rheumatoid	171:180	arg1	arthritis					182:190	rheumatoid arthritis	171:190	rheumatoid arthritis	171:190	Anti-citrullinated protein antibodies (ACPAs) are involved in the pathogenesis of rheumatoid arthritis.
35628640	5	20	theme	F	704:704	arg1	fragment					711:718	an F(ab)2 fragment	701:718	an F(ab)2 fragment of anti-human IgG	701:736	Immunocomplexes (IC) were formed by adding an F(ab)2 fragment of anti-human IgG.
35628640	9	21	theme	TNFα	1281:1284	arg1	release					1286:1292	TNFα release	1281:1292	TNFα release	1281:1292	Functional assays show that dimerized ACPA-IgG significantly enhances TNFα release in an FcγRI-dependent manner, whereas healthy IgG does not.
35628640	10	22	theme	glycoform	1431:1439	arg1	intensities					1409:1419	the relative intensities	1396:1419	the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties	1396:1496	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	3	23	theme	inflammatory	514:525	arg1	markers					527:533	inflammatory markers	514:533	inflammatory markers in RA	514:539	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	6	24	used	used	755:758	arg2	cells					744:748	U937 cells	739:748	U937 cells	739:748	U937 cells were used to monitor the binding of IC to FcγR and to trigger TNFα release determined by ELISA.
35628640	3	25	theme	control	359:365	arg1	IgG					367:369	control IgG	359:369	control IgG	359:369	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	10	26	theme	G0	1428:1429	arg1	glycoform					1431:1439	the G0 glycoform	1424:1439	the G0 glycoform	1424:1439	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	3	27	theme	TNFα	410:413	arg1	production					415:424	TNFα production	410:424	TNFα production	410:424	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	9	28	theme	dimerized	1239:1247	arg1	ACPA-IgG					1249:1256	dimerized ACPA-IgG	1239:1256	dimerized ACPA-IgG	1239:1256	Functional assays show that dimerized ACPA-IgG significantly enhances TNFα release in an FcγRI-dependent manner, whereas healthy IgG does not.
35628640	7	29	theme	site-specific	1003:1015	arg1	N-glycosylation					1017:1031	site-specific N-glycosylation	1003:1031	site-specific N-glycosylation using nano-UHPLC-MS/MS	1003:1054	To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS.
35628640	1	30	theme	arthritis	182:190	arg1	pathogenesis					155:166	the pathogenesis	151:166	the pathogenesis of rheumatoid arthritis	151:190	Anti-citrullinated protein antibodies (ACPAs) are involved in the pathogenesis of rheumatoid arthritis.
35628640	0	31	theme	IgG	12:14	arg1	N-Glycosylation					26:40	IgG Fc Region N-Glycosylation	12:40	IgG Fc Region N-Glycosylation	12:40	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.
35628640	7	32	theme	glycan	857:862	arg1	profiles					864:871	glycan profiles	857:871	glycan profiles	857:871	To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS.
35628640	8	33	theme	inflammation-related	1153:1172	arg1	RF					1207:1208	RF	1207:1208	RF	1207:1208	We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF.
35628640	8	33	theme	inflammation-related	1153:1172	arg1	ESR					1198:1200	ESR	1198:1200	ESR	1198:1200	We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF.
35628640	8	33	theme	inflammation-related	1153:1172	arg1	parameters					1174:1183	inflammation-related parameters	1153:1183	inflammation-related parameters such as CRP, ESR, and RF	1153:1208	We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF.
35628640	8	33	theme	inflammation-related	1153:1172	arg1	CRP					1193:1195	CRP	1193:1195	CRP	1193:1195	We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF.
35628640	1	34	theme	Anti-citrullinated	89:106	arg1	ACPAs					128:132	ACPAs	128:132	ACPAs	128:132	Anti-citrullinated protein antibodies (ACPAs) are involved in the pathogenesis of rheumatoid arthritis.
35628640	1	34	theme	Anti-citrullinated	89:106	arg1	antibodies					116:125	Anti-citrullinated protein antibodies	89:125	Anti-citrullinated protein antibodies (ACPAs)	89:133	Anti-citrullinated protein antibodies (ACPAs) are involved in the pathogenesis of rheumatoid arthritis.
35628640	10	35	theme	TNFα	1354:1357	arg1	production					1359:1368	TNFα production	1354:1368	TNFα production	1354:1368	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	3	36	theme	IgG	367:369	arg1	level					340:344	the relative sialylation and galactosylation level	295:344	the relative sialylation and galactosylation level of ACPAs and control IgG	295:369	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	9	37	theme	FcγRI-dependent	1300:1314	arg1	manner					1316:1321	an FcγRI-dependent manner	1297:1321	an FcγRI-dependent manner	1297:1321	Functional assays show that dimerized ACPA-IgG significantly enhances TNFα release in an FcγRI-dependent manner, whereas healthy IgG does not.
35628640	3	38	theme	study	273:277	arg1	aim					261:263	The aim	257:263	The aim of this study	257:277	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	1	39	theme	protein	108:114	arg1	ACPAs					128:132	ACPAs	128:132	ACPAs	128:132	Anti-citrullinated protein antibodies (ACPAs) are involved in the pathogenesis of rheumatoid arthritis.
35628640	1	39	theme	protein	108:114	arg1	antibodies					116:125	Anti-citrullinated protein antibodies	89:125	Anti-citrullinated protein antibodies (ACPAs)	89:133	Anti-citrullinated protein antibodies (ACPAs) are involved in the pathogenesis of rheumatoid arthritis.
35628640	4	40	theme	patients	597:604	arg1	sera					563:566	sera	563:566	sera of healthy volunteers and RA patients	563:604	We isolated IgG from sera of healthy volunteers and RA patients, and purified ACPAs on a citrulline-peptide column.
35628640	0	41	theme	Region	19:24	arg1	N-Glycosylation					26:40	IgG Fc Region N-Glycosylation	12:40	IgG Fc Region N-Glycosylation	12:40	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.
35628640	4	42	theme	citrulline-peptide	631:648	arg1	column					650:655	a citrulline-peptide column	629:655	a citrulline-peptide column	629:655	We isolated IgG from sera of healthy volunteers and RA patients, and purified ACPAs on a citrulline-peptide column.
35628640	7	43	theme	glycopeptides	960:972	arg1	patterns					948:955	the glycosylation patterns	930:955	the glycosylation patterns of glycopeptides	930:972	To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS.
35628640	6	44	theme	IC	786:787	arg1	binding					775:781	the binding	771:781	the binding of IC to FcγR	771:795	U937 cells were used to monitor the binding of IC to FcγR and to trigger TNFα release determined by ELISA.
35628640	2	45	theme	IgG	241:243	arg1	Fc					245:246	ACPA-IgG and healthy IgG Fc	220:246	ACPA-IgG and healthy IgG Fc	220:246	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc differs.
35628640	0	46	theme	Fc	16:17	arg1	N-Glycosylation					26:40	IgG Fc Region N-Glycosylation	12:40	IgG Fc Region N-Glycosylation	12:40	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.
35628640	8	47	theme	sialylation	1076:1086	arg1	levels					1108:1113	both sialylation and galactosylation levels	1071:1113	both sialylation and galactosylation levels of ACPA-IgG	1071:1125	We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF.
35628640	2	48	theme	healthy	233:239	arg1	Fc					245:246	ACPA-IgG and healthy IgG Fc	220:246	ACPA-IgG and healthy IgG Fc	220:246	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc differs.
35628640	5	49	theme	anti-human	723:732	arg1	IgG					734:736	anti-human IgG	723:736	anti-human IgG	723:736	Immunocomplexes (IC) were formed by adding an F(ab)2 fragment of anti-human IgG.
35628640	5	50	theme	IgG	734:736	arg1	fragment					711:718	an F(ab)2 fragment	701:718	an F(ab)2 fragment of anti-human IgG	701:736	Immunocomplexes (IC) were formed by adding an F(ab)2 fragment of anti-human IgG.
35628640	0	51	theme	N-Glycosylation	26:40	arg1	Role					4:7	The Role	0:7	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.	0:87	The Role of IgG Fc Region N-Glycosylation in the Pathomechanism of Rheumatoid Arthritis.
35628640	9	52	theme	healthy	1332:1338	arg1	IgG					1340:1342	healthy IgG	1332:1342	healthy IgG	1332:1342	Functional assays show that dimerized ACPA-IgG significantly enhances TNFα release in an FcγRI-dependent manner, whereas healthy IgG does not.
35628640	10	53	theme	acid	1484:1487	arg1	moieties					1489:1496	terminal sialic acid moieties	1468:1496	terminal sialic acid moieties	1468:1496	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	2	54	theme	ACPA-IgG	220:227	arg1	Fc					245:246	ACPA-IgG and healthy IgG Fc	220:246	ACPA-IgG and healthy IgG Fc	220:246	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc differs.
35628640	3	55	from	RA	538:539	arg1	composition					484:494	the composition	480:494	the composition of Fc glycans and inflammatory markers in RA	480:539	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	10	56	theme	sialic	1477:1482	arg1	moieties					1489:1496	terminal sialic acid moieties	1468:1496	terminal sialic acid moieties	1468:1496	TNFα production inversely correlates with the relative intensities of the G0 glycoform, which lacks galactose and terminal sialic acid moieties.
35628640	7	57	gly	glycosylation	934:946	arg1	glycopeptides					960:972	glycopeptides	960:972	glycopeptides	960:972	To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS.
35628640	4	58	theme	volunteers	579:588	arg1	sera					563:566	sera	563:566	sera of healthy volunteers and RA patients	563:604	We isolated IgG from sera of healthy volunteers and RA patients, and purified ACPAs on a citrulline-peptide column.
35628640	7	59	theme	glycosylation	934:946	arg1	patterns					948:955	the glycosylation patterns	930:955	the glycosylation patterns of glycopeptides	930:972	To analyze glycan profiles, control IgG and ACPA-IgG were digested with trypsin and the glycosylation patterns of glycopeptides were analyzed by determining site-specific N-glycosylation using nano-UHPLC-MS/MS.
35628640	2	60	theme	N-glycosylation	193:207	arg1	pattern					209:215	N-glycosylation pattern	193:215	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc	193:246	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc differs.
35628640	4	61	theme	healthy	571:577	arg1	volunteers					579:588	healthy volunteers	571:588	healthy volunteers	571:588	We isolated IgG from sera of healthy volunteers and RA patients, and purified ACPAs on a citrulline-peptide column.
35628640	3	62	theme	Fc	499:500	arg1	glycans					502:508	Fc glycans	499:508	Fc glycans	499:508	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	2	63	gly	N-glycosylation	193:207	arg1	Fc					245:246	ACPA-IgG and healthy IgG Fc	220:246	ACPA-IgG and healthy IgG Fc	220:246	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc differs.
35628640	4	64	theme	RA	594:595	arg1	patients					597:604	RA patients	594:604	RA patients	594:604	We isolated IgG from sera of healthy volunteers and RA patients, and purified ACPAs on a citrulline-peptide column.
35628640	3	65	theme	galactosylation	324:338	arg1	level					340:344	the relative sialylation and galactosylation level	295:344	the relative sialylation and galactosylation level of ACPAs and control IgG	295:369	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	2	66	theme	Fc	245:246	arg1	pattern					209:215	N-glycosylation pattern	193:215	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc	193:246	N-glycosylation pattern of ACPA-IgG and healthy IgG Fc differs.
35628640	3	67	theme	glycans	502:508	arg1	composition					484:494	the composition	480:494	the composition of Fc glycans and inflammatory markers in RA	480:539	The aim of this study is to determine the relative sialylation and galactosylation level of ACPAs and control IgG to assess their capability of inducing TNFα production, and furthermore, to analyze the correlations between the composition of Fc glycans and inflammatory markers in RA.
35628640	8	68	theme	galactosylation	1092:1106	arg1	levels					1108:1113	both sialylation and galactosylation levels	1071:1113	both sialylation and galactosylation levels of ACPA-IgG	1071:1125	We found that both sialylation and galactosylation levels of ACPA-IgG negatively correlate with inflammation-related parameters such as CRP, ESR, and RF.
36068613	13	0	dep	Double-modified	1673:1687	arg1	scaffolds					1720:1728	sulfonated bacterial cellulose scaffolds	1689:1728	sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs	1689:1763	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	9	1	theme	potential	1174:1182	arg1	function					1195:1202	the potential biological function	1170:1202	the potential biological function of FGFR2 by bioinformatics and a cytokine chip	1170:1249	At the same time, we detected the potential biological function of FGFR2 by bioinformatics and a cytokine chip.
36068613	7	2	theme	-modified	891:899	arg1	ADSCs					901:905	Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs	848:905	Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs	848:905	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	2	3	theme	tissue	316:321	arg1	engineering					323:333	tissue engineering	316:333	tissue engineering	316:333	The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem.
36068613	9	4	theme	FGFR2	1207:1211	arg1	function					1195:1202	the potential biological function	1170:1202	the potential biological function of FGFR2 by bioinformatics and a cytokine chip	1170:1249	At the same time, we detected the potential biological function of FGFR2 by bioinformatics and a cytokine chip.
36068613	13	5	theme	urethral	1816:1823	arg1	strictures					1825:1834	urethral strictures	1816:1834	urethral strictures	1816:1834	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	6	6	theme	materials	813:821	arg1	properties					793:802	the relevant mechanical properties	769:802	the relevant mechanical properties of these materials	769:821	We tested the relevant mechanical properties of these materials.
36068613	7	7	theme	Factor	866:871	arg1	Receptor					873:880	Fibroblast Growth Factor Receptor 2	848:882	Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs	848:905	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	7	7	theme	Factor	866:871	arg1	FGFR2					885:889	FGFR2	885:889	FGFR2	885:889	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	8	8	theme	New	1018:1020	arg1	model					1037:1041	the male New Zealand rabbit model	1009:1041	the male New Zealand rabbit model	1009:1041	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	12	9	from	ADSCs	1654:1658	arg1	secretion					1591:1599	the secretion	1587:1599	the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs	1587:1658	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	7	10	theme	Fibroblast	848:857	arg1	Receptor					873:880	Fibroblast Growth Factor Receptor 2	848:882	Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs	848:905	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	7	10	theme	Fibroblast	848:857	arg1	FGFR2					885:889	FGFR2	885:889	FGFR2	885:889	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	1	11	theme	thorny	235:240	arg1	problems					252:259	the thorny difficult problems	231:259	the thorny difficult problems in the field of urology	231:283	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	1	12	theme	problems	252:259	arg1	one					224:226	one	224:226	one	224:226	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	1	12	theme	problems	252:259	arg1	problems					252:259	the thorny difficult problems	231:259	the thorny difficult problems in the field of urology	231:283	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	4	13	link	adipose-derived	515:529	arg1	ADSCs					543:547	ADSCs	543:547	ADSCs	543:547	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	4	13	link	adipose-derived	515:529	arg1	cells					536:540	adipose-derived stem cells	515:540	adipose-derived stem cells (ADSCs)	515:548	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	8	14	dep	examinations	1091:1102	arg1	3 months					1110:1117	3 months	1110:1117	3 months	1110:1117	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	8	14	dep	examinations	1091:1102	arg1	1					1104:1104	1	1104:1104	1	1104:1104	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	12	15	theme	Growth	1625:1630	arg1	Factor					1632:1637	Vascular Endothelial Growth Factor A (VEGFA)	1604:1647	Vascular Endothelial Growth Factor A (VEGFA)	1604:1647	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	0	16	theme	scaffold	71:78	arg1	material					80:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	1	17	theme	Urethral	182:189	arg1	stricture					191:199	BACKGROUND Urethral stricture	171:199	BACKGROUND Urethral stricture	171:199	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	5	18	dep	cell	726:729	arg1	attachment					731:740	attachment	731:740	attachment	731:740	METHODS First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation.
36068613	5	18	dep	cell	726:729	arg1	degradation					746:756	degradation	746:756	degradation	746:756	METHODS First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation.
36068613	8	19	theme	rabbit	1030:1035	arg1	model					1037:1041	the male New Zealand rabbit model	1009:1041	the male New Zealand rabbit model	1009:1041	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	13	20	theme	new	1773:1775	arg1	sight					1777:1781	new sight	1773:1781	new sight	1773:1781	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	0	21	theme	FGFR2-modified	101:114	arg1	cells					137:141	FGFR2-modified adipose-derived stem cells	101:141	FGFR2-modified adipose-derived stem cells in urethral reconstruction	101:168	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	0	22	theme	stem	132:135	arg1	cells					137:141	FGFR2-modified adipose-derived stem cells	101:141	FGFR2-modified adipose-derived stem cells in urethral reconstruction	101:168	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	7	23	theme	tissue	950:955	arg1	engineering					957:967	tissue engineering	950:967	tissue engineering	950:967	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	4	24	theme	adipose-derived	515:529	arg1	ADSCs					543:547	ADSCs	543:547	ADSCs	543:547	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	4	24	theme	adipose-derived	515:529	arg1	cells					536:540	adipose-derived stem cells	515:540	adipose-derived stem cells (ADSCs)	515:548	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	7	25	attach	attached	839:846	arg2	we					836:837	we	836:837	we	836:837	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	7	25	attach	attached	839:846	arg1	material					914:921	the material	910:921	the material	910:921	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	11	26	theme	therapeutic	1436:1446	arg1	methods					1448:1454	therapeutic methods	1436:1454	therapeutic methods	1436:1454	The new composite in this study provides new insight and therapeutic methods for urethral reconstruction.
36068613	0	27	theme	Clinical	0:7	arg1	application					9:19	Clinical application	0:19	Clinical application of a double-modified sulfated bacterial cellulose scaffold material	0:87	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	5	28	from	sulfonation	671:681	arg1	study					691:695	this study	686:695	this study	686:695	METHODS First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation.
36068613	4	29	theme	healing	576:582	arg1	ability					584:590	their wound healing ability	564:590	their wound healing ability	564:590	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	13	30	theme	Double-modified	1673:1687	arg1	CONCLUSIONS					1661:1671	CONCLUSIONS	1661:1671	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs	1661:1763	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	5	31	from	drilling	658:665	arg1	study					691:695	this study	686:695	this study	686:695	METHODS First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation.
36068613	3	32	theme	excellent	435:443	arg1	cellulose					414:422	Bacterial cellulose	404:422	Bacterial cellulose (BC)	404:427	Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity.
36068613	3	32	theme	excellent	435:443	arg1	biomaterial					445:455	an excellent biomaterial	432:455	an excellent biomaterial due to its accessibility and strong plasticity	432:502	Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity.
36068613	13	33	theme	bacterial	1700:1708	arg1	scaffolds					1720:1728	sulfonated bacterial cellulose scaffolds	1689:1728	sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs	1689:1763	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	10	34	theme	excellent	1300:1308	arg1	repairability					1310:1322	excellent repairability	1300:1322	excellent repairability	1300:1322	RESULTS The results show that the composite has excellent repairability and that this ability is correlated with angiogenesis.
36068613	0	35	theme	double-modified	26:40	arg1	material					80:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	0	36	theme	bacterial	51:59	arg1	material					80:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	6	37	theme	relevant	773:780	arg1	properties					793:802	the relevant mechanical properties	769:802	the relevant mechanical properties of these materials	769:821	We tested the relevant mechanical properties of these materials.
36068613	2	38	theme	therapeutic	366:376	arg1	thinking					378:385	new therapeutic thinking	362:385	new therapeutic thinking	362:385	The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem.
36068613	0	39	link	adipose-derived	116:130	arg1	cells					137:141	FGFR2-modified adipose-derived stem cells	101:141	FGFR2-modified adipose-derived stem cells in urethral reconstruction	101:168	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	11	40	theme	new	1383:1385	arg1	composite					1387:1395	The new composite	1379:1395	The new composite in this study	1379:1409	The new composite in this study provides new insight and therapeutic methods for urethral reconstruction.
36068613	1	41	theme	urology	277:283	arg1	field					268:272	the field	264:272	the field of urology	264:283	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	11	42	theme	new	1420:1422	arg1	insight					1424:1430	new insight	1420:1430	new insight	1420:1430	The new composite in this study provides new insight and therapeutic methods for urethral reconstruction.
36068613	9	43	theme	biological	1184:1193	arg1	function					1195:1202	the potential biological function	1170:1202	the potential biological function of FGFR2 by bioinformatics and a cytokine chip	1170:1249	At the same time, we detected the potential biological function of FGFR2 by bioinformatics and a cytokine chip.
36068613	2	44	theme	engineering	323:333	arg1	development					301:311	The continuous development	286:311	The continuous development of tissue engineering and biomaterials	286:350	The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem.
36068613	8	45	theme	male	1013:1016	arg1	model					1037:1041	the male New Zealand rabbit model	1009:1041	the male New Zealand rabbit model	1009:1041	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	8	46	theme	Zealand	1022:1028	arg1	model					1037:1041	the male New Zealand rabbit model	1009:1041	the male New Zealand rabbit model	1009:1041	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	0	47	theme	urethral	146:153	arg1	reconstruction					155:168	urethral reconstruction	146:168	urethral reconstruction	146:168	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	2	48	theme	biomaterials	339:350	arg1	development					301:311	The continuous development	286:311	The continuous development of tissue engineering and biomaterials	286:350	The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem.
36068613	7	49	theme	Receptor	873:880	arg1	ADSCs					901:905	Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs	848:905	Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs	848:905	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	6	50	theme	mechanical	782:791	arg1	properties					793:802	the relevant mechanical properties	769:802	the relevant mechanical properties of these materials	769:821	We tested the relevant mechanical properties of these materials.
36068613	9	51	theme	cytokine	1237:1244	arg1	chip					1246:1249	a cytokine chip	1235:1249	a cytokine chip	1235:1249	At the same time, we detected the potential biological function of FGFR2 by bioinformatics and a cytokine chip.
36068613	7	52	theme	Growth	859:864	arg1	Receptor					873:880	Fibroblast Growth Factor Receptor 2	848:882	Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs	848:905	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	7	52	theme	Growth	859:864	arg1	FGFR2					885:889	FGFR2	885:889	FGFR2	885:889	After that, we attached Fibroblast Growth Factor Receptor 2 (FGFR2)-modified ADSCs to the material to construct a urethra for tissue engineering.
36068613	1	53	theme	difficult	242:250	arg1	problems					252:259	the thorny difficult problems	231:259	the thorny difficult problems in the field of urology	231:283	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	12	54	theme	Endothelial	1613:1623	arg1	Factor					1632:1637	Vascular Endothelial Growth Factor A (VEGFA)	1604:1647	Vascular Endothelial Growth Factor A (VEGFA)	1604:1647	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	1	55	from	problems	252:259	arg1	field					268:272	the field	264:272	the field of urology	264:283	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	12	56	theme	Factor	1632:1637	arg1	secretion					1591:1599	the secretion	1587:1599	the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs	1587:1658	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	0	57	from	cells	137:141	arg1	reconstruction					155:168	urethral reconstruction	146:168	urethral reconstruction	146:168	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	11	58	from	composite	1387:1395	arg1	study					1405:1409	this study	1400:1409	this study	1400:1409	The new composite in this study provides new insight and therapeutic methods for urethral reconstruction.
36068613	2	59	theme	continuous	290:299	arg1	development					301:311	The continuous development	286:311	The continuous development of tissue engineering and biomaterials	286:350	The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem.
36068613	0	60	theme	material	80:87	arg1	application					9:19	Clinical application	0:19	Clinical application of a double-modified sulfated bacterial cellulose scaffold material	0:87	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	1	61	theme	BACKGROUND	171:180	arg1	stricture					191:199	BACKGROUND Urethral stricture	171:199	BACKGROUND Urethral stricture	171:199	BACKGROUND Urethral stricture and reconstruction are one of the thorny difficult problems in the field of urology.
36068613	12	62	theme	tissue	1560:1565	arg1	repair					1567:1572	tissue repair	1560:1572	tissue repair	1560:1572	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	0	63	theme	adipose-derived	116:130	arg1	cells					137:141	FGFR2-modified adipose-derived stem cells	101:141	FGFR2-modified adipose-derived stem cells in urethral reconstruction	101:168	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	10	64	contain	has	1296:1298	arg1	composite					1286:1294	the composite	1282:1294	the composite	1282:1294	RESULTS The results show that the composite has excellent repairability and that this ability is correlated with angiogenesis.
36068613	10	64	contain	has	1296:1298	arg2	repairability					1310:1322	excellent repairability	1300:1322	excellent repairability	1300:1322	RESULTS The results show that the composite has excellent repairability and that this ability is correlated with angiogenesis.
36068613	10	65	dep	RESULTS	1252:1258	arg1	show					1272:1275	show	1272:1275	show that the composite has excellent repairability and that this ability is correlated with angiogenesis	1272:1376	RESULTS The results show that the composite has excellent repairability and that this ability is correlated with angiogenesis.
36068613	3	66	theme	Bacterial	404:412	arg1	BC					425:426	BC	425:426	BC	425:426	Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity.
36068613	3	66	theme	Bacterial	404:412	arg1	cellulose					414:422	Bacterial cellulose	404:422	Bacterial cellulose (BC)	404:427	Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity.
36068613	3	66	theme	Bacterial	404:412	arg1	biomaterial					445:455	an excellent biomaterial	432:455	an excellent biomaterial due to its accessibility and strong plasticity	432:502	Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity.
36068613	13	67	with	patients	1802:1809	arg1	strictures					1825:1834	urethral strictures	1816:1834	urethral strictures	1816:1834	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	8	68	theme	immunohistochemical	1059:1077	arg1	examinations					1091:1102	immunohistochemical and imaging examinations 1 and 3 months	1059:1117	examinations	1091:1102	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	5	69	theme	physical	649:656	arg1	drilling					658:665	physical drilling	649:665	physical drilling	649:665	METHODS First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation.
36068613	4	70	theme	stem	531:534	arg1	ADSCs					543:547	ADSCs	543:547	ADSCs	543:547	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	4	70	theme	stem	531:534	arg1	cells					536:540	adipose-derived stem cells	515:540	adipose-derived stem cells (ADSCs)	515:548	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	8	71	theme	imaging	1083:1089	arg1	examinations					1091:1102	immunohistochemical and imaging examinations 1 and 3 months	1059:1117	examinations	1091:1102	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	13	72	theme	FGFR2-modified	1744:1757	arg1	ADSCs					1759:1763	FGFR2-modified ADSCs	1744:1763	FGFR2-modified ADSCs	1744:1763	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	5	73	used	used	644:647	arg2	we					641:642	we	641:642	we	641:642	METHODS First, we used physical drilling and sulfonation in this study to make BC more conducive to cell attachment and degradation.
36068613	4	74	theme	directional	600:610	arg1	modification					612:623	directional modification	600:623	directional modification	600:623	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	13	75	theme	sulfonated	1689:1698	arg1	scaffolds					1720:1728	sulfonated bacterial cellulose scaffolds	1689:1728	sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs	1689:1763	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	11	76	theme	urethral	1460:1467	arg1	reconstruction					1469:1482	urethral reconstruction	1460:1482	urethral reconstruction	1460:1482	The new composite in this study provides new insight and therapeutic methods for urethral reconstruction.
36068613	8	77	contain	carried	1047:1053	arg2	examinations					1091:1102	immunohistochemical and imaging examinations 1 and 3 months	1059:1117	examinations	1091:1102	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	8	77	contain	carried	1047:1053	arg1	we					981:982	we	981:982	we	981:982	Afterward, we verified this finding in the male New Zealand rabbit model and carried out immunohistochemical and imaging examinations 1 and 3 months after the operation.
36068613	0	78	theme	sulfated	42:49	arg1	material					80:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	13	79	theme	cellulose	1710:1718	arg1	scaffolds					1720:1728	sulfonated bacterial cellulose scaffolds	1689:1728	sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs	1689:1763	CONCLUSIONS Double-modified sulfonated bacterial cellulose scaffolds combined with FGFR2-modified ADSCs provide new sight and treatments for patients with urethral strictures.
36068613	3	80	theme	strong	486:491	arg1	plasticity					493:502	strong plasticity	486:502	strong plasticity	486:502	Bacterial cellulose (BC) is an excellent biomaterial due to its accessibility and strong plasticity.
36068613	0	81	theme	cellulose	61:69	arg1	material					80:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	a double-modified sulfated bacterial cellulose scaffold material	24:87	Clinical application of a double-modified sulfated bacterial cellulose scaffold material loaded with FGFR2-modified adipose-derived stem cells in urethral reconstruction.
36068613	4	82	theme	wound	570:574	arg1	healing					576:582	their wound healing	564:582	their wound healing ability	564:590	Moreover, adipose-derived stem cells (ADSCs) could enhance their wound healing ability through directional modification.
36068613	9	83	theme	same	1147:1150	arg1	time					1152:1155	the same time	1143:1155	the same time	1143:1155	At the same time, we detected the potential biological function of FGFR2 by bioinformatics and a cytokine chip.
36068613	12	84	theme	preliminary	1489:1499	arg1	mechanism					1501:1509	The preliminary mechanism	1485:1509	The preliminary mechanism	1485:1509	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	2	85	theme	new	362:364	arg1	thinking					378:385	new therapeutic thinking	362:385	new therapeutic thinking	362:385	The continuous development of tissue engineering and biomaterials has given new therapeutic thinking to this problem.
36068613	12	86	dep	Factor	1632:1637	arg1	VEGFA					1642:1646	VEGFA	1642:1646	VEGFA	1642:1646	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	12	86	dep	Factor	1632:1637	arg1	A					1639:1639	A	1639:1639	Vascular Endothelial Growth Factor A (VEGFA)	1604:1647	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36068613	12	87	theme	Vascular	1604:1611	arg1	Factor					1632:1637	Vascular Endothelial Growth Factor A (VEGFA)	1604:1647	Vascular Endothelial Growth Factor A (VEGFA)	1604:1647	The preliminary mechanism showed that FGFR2 could promote angiogenesis and tissue repair by promoting the secretion of Vascular Endothelial Growth Factor A (VEGFA) from ADSCs.
36192963	11	0	theme	nuclear	1541:1547	arg1	B					1562:1562	nuclear factor-kappa B	1541:1562	nuclear factor-kappa B signaling pathways	1541:1581	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	8	1	theme	E2	1098:1099	arg1	production					1053:1062	the production	1049:1062	the production of nitric oxide and prostaglandin E2	1049:1099	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	10	2	theme	anti-inflammatory	1337:1353	arg1	activity					1355:1362	stronger anti-inflammatory activity	1328:1362	stronger anti-inflammatory activity	1328:1362	Additionally, BCWE exhibited stronger anti-inflammatory activity than BYWE.
36192963	8	3	theme	cyclooxygenase-2	1181:1196	arg1	levels					1135:1140	the expression levels	1120:1140	the expression levels of inducible nitric oxide synthase and cyclooxygenase-2	1120:1196	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	11	4	theme	pathways	1574:1581	arg1	activation					1440:1449	the activation	1436:1449	the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways	1436:1581	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	2	5	theme	anti-inflammatory	241:257	arg1	activity					259:266	The anti-inflammatory activity	237:266	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms	237:315	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms remain virtually unknown.
36192963	1	6	theme	non-conventional	152:167	arg1	tea					120:122	Cocoa tea	114:122	Cocoa tea (Camellia ptilophylla)	114:145	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	6	theme	non-conventional	152:167	arg1	variety					173:179	a non-conventional tea variety	150:179	a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG)	150:234	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	8	7	theme	expression	1124:1133	arg1	levels					1135:1140	the expression levels	1120:1140	the expression levels of inducible nitric oxide synthase and cyclooxygenase-2	1120:1196	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	11	8	theme	B	1562:1562	arg1	pathways					1574:1581	nuclear factor-kappa B signaling pathways	1541:1581	nuclear factor-kappa B signaling pathways	1541:1581	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	12	9	theme	black	1612:1616	arg1	tea					1624:1626	black cocoa tea	1612:1626	black cocoa tea	1612:1626	These findings suggest that black cocoa tea can be developed into a promising functional beverage with anti-inflammatory property.
36192963	2	10	theme	cocoa	277:281	arg1	tea					283:285	black cocoa tea	271:285	black cocoa tea	271:285	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms remain virtually unknown.
36192963	1	11	theme	low	186:188	arg1	caffeine					190:197	low caffeine	186:197	low caffeine	186:197	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	8	12	theme	nitric	1155:1160	arg1	synthase					1168:1175	inducible nitric oxide synthase	1145:1175	inducible nitric oxide synthase	1145:1175	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	5	13	theme	total	656:660	arg1	polyphenols					662:672	total polyphenols	656:672	total polyphenols in BCWE	656:680	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	5	14	from	polyphenols	662:672	arg1	BCWE					677:680	BCWE	677:680	BCWE	677:680	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	8	15	theme	synthase	1168:1175	arg1	levels					1135:1140	the expression levels	1120:1140	the expression levels of inducible nitric oxide synthase and cyclooxygenase-2	1120:1196	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	5	16	theme	acid	629:632	arg1	higher					701:706	higher	701:706	higher	701:706	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	5	16	theme	acid	629:632	arg1	polyphenols					662:672	total polyphenols	656:672	total polyphenols in BCWE	656:680	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	5	16	theme	acid	629:632	arg1	contents					610:617	The contents	606:617	The contents of gallic acid	606:632	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	5	16	theme	acid	629:632	arg1	catechins					641:649	total catechins	635:649	total catechins	635:649	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	1	17	theme	Cocoa	114:118	arg1	tea					120:122	Cocoa tea	114:122	Cocoa tea (Camellia ptilophylla)	114:145	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	17	theme	Cocoa	114:118	arg1	ptilophylla					134:144	Camellia ptilophylla	125:144	Camellia ptilophylla	125:144	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	17	theme	Cocoa	114:118	arg1	variety					173:179	a non-conventional tea variety	150:179	a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG)	150:234	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	3	18	from	tea	476:478	arg1	extracts					423:430	water extracts	417:430	water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE)	417:485	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	18	from	tea	476:478	arg1	composition					371:381	chemical composition	362:381	chemical composition	362:381	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	18	from	tea	476:478	arg1	activity					405:412	anti-inflammatory activity	387:412	anti-inflammatory activity	387:412	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	11	19	theme	kinases	1480:1486	arg1	activation					1440:1449	the activation	1436:1449	the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways	1436:1581	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	5	20	theme	total	635:639	arg1	catechins					641:649	total catechins	635:649	total catechins	635:649	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	6	21	contain	contained	751:759	arg2	little					763:768	little	763:768	little	763:768	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	6	21	contain	contained	751:759	arg1	BYWE					746:749	BYWE	746:749	BYWE	746:749	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	11	22	theme	mitogen-activated	1454:1470	arg1	kinases					1480:1486	mitogen-activated protein kinases	1454:1486	mitogen-activated protein kinases	1454:1486	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	3	23	from	composition	371:381	arg1	tea					476:478	Yunnan Daye tea	464:478	Yunnan Daye tea (BYWE)	464:485	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	23	from	composition	371:381	arg1	BCWE					454:457	BCWE	454:457	BCWE	454:457	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	23	from	composition	371:381	arg1	tea					449:451	black cocoa tea	437:451	black cocoa tea (BCWE)	437:458	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	23	from	composition	371:381	arg1	BYWE					481:484	BYWE	481:484	BYWE	481:484	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	11	24	theme	B	1534:1534	arg1	activation					1440:1449	the activation	1436:1449	the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways	1436:1581	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	0	25	theme	cocoa	80:84	arg1	tea					86:88	black cocoa tea	74:88	black cocoa tea (Camellia ptilophylla)	74:111	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	25	theme	cocoa	80:84	arg1	ptilophylla					100:110	Camellia ptilophylla	91:110	Camellia ptilophylla	91:110	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	9	26	theme	tumor	1241:1245	arg1	necrosis					1247:1254	tumor necrosis factor-α and interleukin-6	1241:1281	tumor necrosis factor-α and interleukin-6	1241:1281	They also markedly reduced the release of tumor necrosis factor-α and interleukin-6 at a high dose.
36192963	11	27	theme	phosphatidylinositol-3-kinase/protein	1489:1525	arg1	B					1534:1534	phosphatidylinositol-3-kinase/protein kinase B	1489:1534	phosphatidylinositol-3-kinase/protein kinase B	1489:1534	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	12	28	theme	functional	1662:1671	arg1	beverage					1673:1680	a promising functional beverage	1650:1680	a promising functional beverage with anti-inflammatory property	1650:1712	These findings suggest that black cocoa tea can be developed into a promising functional beverage with anti-inflammatory property.
36192963	3	29	dep	composition	371:381	arg1	the					358:360	the	358:360	the	358:360	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	30	theme	extracts	423:430	arg1	composition					371:381	chemical composition	362:381	chemical composition	362:381	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	30	theme	extracts	423:430	arg1	activity					405:412	anti-inflammatory activity	387:412	anti-inflammatory activity	387:412	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	8	31	theme	lipopolysaccharide-induced	973:998	arg1	cells					1009:1013	lipopolysaccharide-induced RAW264.7 cells	973:1013	lipopolysaccharide-induced RAW264.7 cells	973:1013	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	0	32	from	activity	43:50	arg1	tea					86:88	black cocoa tea	74:88	black cocoa tea (Camellia ptilophylla)	74:111	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	32	from	activity	43:50	arg1	ptilophylla					100:110	Camellia ptilophylla	91:110	Camellia ptilophylla	91:110	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	33	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	9	34	dep	necrosis	1247:1254	arg1	interleukin-6					1269:1281	interleukin-6	1269:1281	interleukin-6	1269:1281	They also markedly reduced the release of tumor necrosis factor-α and interleukin-6 at a high dose.
36192963	9	34	dep	necrosis	1247:1254	arg1	factor-α					1256:1263	factor-α	1256:1263	factor-α	1256:1263	They also markedly reduced the release of tumor necrosis factor-α and interleukin-6 at a high dose.
36192963	10	35	theme	stronger	1328:1335	arg1	activity					1355:1362	stronger anti-inflammatory activity	1328:1362	stronger anti-inflammatory activity	1328:1362	Additionally, BCWE exhibited stronger anti-inflammatory activity than BYWE.
36192963	3	36	theme	anti-inflammatory	387:403	arg1	activity					405:412	anti-inflammatory activity	387:412	anti-inflammatory activity	387:412	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	6	37	dep	little	763:768	arg1	theaflavins					781:791	theaflavins	781:791	theaflavins	781:791	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	6	37	dep	little	763:768	arg1	theabrownins					797:808	theabrownins	797:808	theabrownins	797:808	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	3	38	theme	black	437:441	arg1	BCWE					454:457	BCWE	454:457	BCWE	454:457	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	38	theme	black	437:441	arg1	tea					449:451	black cocoa tea	437:451	black cocoa tea (BCWE)	437:458	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	5	39	from	contents	610:617	arg1	BCWE					677:680	BCWE	677:680	BCWE	677:680	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	12	40	with	beverage	1673:1680	arg1	property					1705:1712	anti-inflammatory property	1687:1712	anti-inflammatory property	1687:1712	These findings suggest that black cocoa tea can be developed into a promising functional beverage with anti-inflammatory property.
36192963	6	41	located	observed	857:864	arg1	thearubigins					869:880	thearubigins	869:880	thearubigins	869:880	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	6	41	located	observed	857:864	arg2	difference					842:851	no significant difference	827:851	no significant difference	827:851	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	0	42	theme	water	55:59	arg1	extract					61:67	water extract	55:67	water extract from black cocoa tea (Camellia ptilophylla)	55:111	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	1	43	with	variety	173:179	arg1	gallate					222:228	high gallocatechin gallate	203:228	high gallocatechin gallate (GCG)	203:234	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	43	with	variety	173:179	arg1	caffeine					190:197	low caffeine	186:197	low caffeine	186:197	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	43	with	variety	173:179	arg1	GCG					231:233	GCG	231:233	GCG	231:233	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	2	44	theme	mechanisms	306:315	arg1	activity					259:266	The anti-inflammatory activity	237:266	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms	237:315	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms remain virtually unknown.
36192963	1	45	theme	high	203:206	arg1	gallate					222:228	high gallocatechin gallate	203:228	high gallocatechin gallate (GCG)	203:234	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	45	theme	high	203:206	arg1	GCG					231:233	GCG	231:233	GCG	231:233	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	3	46	theme	Daye	471:474	arg1	tea					476:478	Yunnan Daye tea	464:478	Yunnan Daye tea (BYWE)	464:485	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	46	theme	Daye	471:474	arg1	BYWE					481:484	BYWE	481:484	BYWE	481:484	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	8	47	theme	oxide	1074:1078	arg1	production					1053:1062	the production	1049:1062	the production of nitric oxide and prostaglandin E2	1049:1099	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	6	48	theme	significant	830:840	arg1	difference					842:851	no significant difference	827:851	no significant difference	827:851	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	4	49	theme	dominant	526:533	arg1	alkaloid					535:542	the dominant alkaloid	522:542	the dominant alkaloid in BCWE and BYWE	522:559	Results showed that the dominant alkaloid in BCWE and BYWE were theobromine and caffeine, respectively.
36192963	4	49	theme	dominant	526:533	arg1	theobromine					566:576	theobromine	566:576	theobromine	566:576	Results showed that the dominant alkaloid in BCWE and BYWE were theobromine and caffeine, respectively.
36192963	7	50	located	found	955:959	arg2	theaflavin					906:915	a novel theaflavin	898:915	a novel theaflavin synthesized from GCG and catechin	898:949	Interestingly, a novel theaflavin synthesized from GCG and catechin was found in BCWE.
36192963	7	50	located	found	955:959	arg1	BCWE					964:967	BCWE	964:967	BCWE	964:967	Interestingly, a novel theaflavin synthesized from GCG and catechin was found in BCWE.
36192963	11	51	theme	factor-kappa	1549:1560	arg1	B					1562:1562	nuclear factor-kappa B	1541:1562	nuclear factor-kappa B signaling pathways	1541:1581	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	2	52	theme	black	271:275	arg1	tea					283:285	black cocoa tea	271:285	black cocoa tea	271:285	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms remain virtually unknown.
36192963	5	53	from	catechins	641:649	arg1	BCWE					677:680	BCWE	677:680	BCWE	677:680	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	8	54	theme	prostaglandin	1084:1096	arg1	E2					1098:1099	prostaglandin E2	1084:1099	prostaglandin E2	1084:1099	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	6	55	theme	tea	732:734	arg1	pigments					736:743	tea pigments	732:743	tea pigments	732:743	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	1	56	theme	tea	169:171	arg1	tea					120:122	Cocoa tea	114:122	Cocoa tea (Camellia ptilophylla)	114:145	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	56	theme	tea	169:171	arg1	variety					173:179	a non-conventional tea variety	150:179	a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG)	150:234	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	12	57	theme	cocoa	1618:1622	arg1	tea					1624:1626	black cocoa tea	1612:1626	black cocoa tea	1612:1626	These findings suggest that black cocoa tea can be developed into a promising functional beverage with anti-inflammatory property.
36192963	11	58	theme	signaling	1564:1572	arg1	pathways					1574:1581	nuclear factor-kappa B signaling pathways	1541:1581	nuclear factor-kappa B signaling pathways	1541:1581	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	2	59	theme	tea	283:285	arg1	activity					259:266	The anti-inflammatory activity	237:266	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms	237:315	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms remain virtually unknown.
36192963	0	60	from	tea	86:88	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	60	from	tea	86:88	arg1	activity					43:50	anti-inflammatory activity	25:50	anti-inflammatory activity	25:50	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	60	from	tea	86:88	arg1	extract					61:67	water extract	55:67	water extract from black cocoa tea (Camellia ptilophylla)	55:111	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	8	61	theme	inducible	1145:1153	arg1	synthase					1168:1175	inducible nitric oxide synthase	1145:1175	inducible nitric oxide synthase	1145:1175	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	5	62	theme	gallic	622:627	arg1	acid					629:632	gallic acid	622:632	gallic acid	622:632	The contents of gallic acid, total catechins, and total polyphenols in BCWE were significantly higher than those in BYWE.
36192963	8	63	theme	oxide	1162:1166	arg1	synthase					1168:1175	inducible nitric oxide synthase	1145:1175	inducible nitric oxide synthase	1145:1175	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	11	64	theme	protein	1472:1478	arg1	kinases					1480:1486	mitogen-activated protein kinases	1454:1486	mitogen-activated protein kinases	1454:1486	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	0	65	theme	black	74:78	arg1	tea					86:88	black cocoa tea	74:88	black cocoa tea (Camellia ptilophylla)	74:111	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	65	theme	black	74:78	arg1	ptilophylla					100:110	Camellia ptilophylla	91:110	Camellia ptilophylla	91:110	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	1	66	theme	Camellia	125:132	arg1	tea					120:122	Cocoa tea	114:122	Cocoa tea (Camellia ptilophylla)	114:145	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	66	theme	Camellia	125:132	arg1	ptilophylla					134:144	Camellia ptilophylla	125:144	Camellia ptilophylla	125:144	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	11	67	theme	kinase	1527:1532	arg1	B					1534:1534	phosphatidylinositol-3-kinase/protein kinase B	1489:1534	phosphatidylinositol-3-kinase/protein kinase B	1489:1534	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	12	68	theme	anti-inflammatory	1687:1703	arg1	property					1705:1712	anti-inflammatory property	1687:1712	anti-inflammatory property	1687:1712	These findings suggest that black cocoa tea can be developed into a promising functional beverage with anti-inflammatory property.
36192963	0	69	from	composition	9:19	arg1	tea					86:88	black cocoa tea	74:88	black cocoa tea (Camellia ptilophylla)	74:111	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	69	from	composition	9:19	arg1	ptilophylla					100:110	Camellia ptilophylla	91:110	Camellia ptilophylla	91:110	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	70	theme	Camellia	91:98	arg1	tea					86:88	black cocoa tea	74:88	black cocoa tea (Camellia ptilophylla)	74:111	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	70	theme	Camellia	91:98	arg1	ptilophylla					100:110	Camellia ptilophylla	91:110	Camellia ptilophylla	91:110	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	3	71	from	activity	405:412	arg1	tea					476:478	Yunnan Daye tea	464:478	Yunnan Daye tea (BYWE)	464:485	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	71	from	activity	405:412	arg1	BCWE					454:457	BCWE	454:457	BCWE	454:457	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	71	from	activity	405:412	arg1	tea					449:451	black cocoa tea	437:451	black cocoa tea (BCWE)	437:458	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	71	from	activity	405:412	arg1	BYWE					481:484	BYWE	481:484	BYWE	481:484	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	7	72	theme	novel	900:904	arg1	theaflavin					906:915	a novel theaflavin	898:915	a novel theaflavin synthesized from GCG and catechin	898:949	Interestingly, a novel theaflavin synthesized from GCG and catechin was found in BCWE.
36192963	9	73	theme	necrosis	1247:1254	arg1	release					1230:1236	the release	1226:1236	the release of tumor necrosis factor-α and interleukin-6	1226:1281	They also markedly reduced the release of tumor necrosis factor-α and interleukin-6 at a high dose.
36192963	11	74	theme	anti-inflammatory	1397:1413	arg1	effect					1415:1420	anti-inflammatory effect	1397:1420	anti-inflammatory effect	1397:1420	Finally, BCWE exerted anti-inflammatory effect by inhibiting the activation of mitogen-activated protein kinases, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways.
36192963	4	75	from	alkaloid	535:542	arg1	BYWE					556:559	BYWE	556:559	BYWE	556:559	Results showed that the dominant alkaloid in BCWE and BYWE were theobromine and caffeine, respectively.
36192963	4	75	from	alkaloid	535:542	arg1	BCWE					547:550	BCWE	547:550	BCWE	547:550	Results showed that the dominant alkaloid in BCWE and BYWE were theobromine and caffeine, respectively.
36192963	8	76	theme	RAW264.7	1000:1007	arg1	cells					1009:1013	lipopolysaccharide-induced RAW264.7 cells	973:1013	lipopolysaccharide-induced RAW264.7 cells	973:1013	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
36192963	12	77	theme	promising	1652:1660	arg1	beverage					1673:1680	a promising functional beverage	1650:1680	a promising functional beverage with anti-inflammatory property	1650:1712	These findings suggest that black cocoa tea can be developed into a promising functional beverage with anti-inflammatory property.
36192963	3	78	theme	chemical	362:369	arg1	composition					371:381	chemical composition	362:381	chemical composition	362:381	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	79	theme	water	417:421	arg1	extracts					423:430	water extracts	417:430	water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE)	417:485	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	0	80	theme	anti-inflammatory	25:41	arg1	activity					43:50	anti-inflammatory activity	25:50	anti-inflammatory activity	25:50	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	9	81	theme	high	1288:1291	arg1	dose					1293:1296	a high dose	1286:1296	a high dose	1286:1296	They also markedly reduced the release of tumor necrosis factor-α and interleukin-6 at a high dose.
36192963	3	82	theme	cocoa	443:447	arg1	BCWE					454:457	BCWE	454:457	BCWE	454:457	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	82	theme	cocoa	443:447	arg1	tea					449:451	black cocoa tea	437:451	black cocoa tea (BCWE)	437:458	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	0	83	theme	extract	61:67	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	0	83	theme	extract	61:67	arg1	activity					43:50	anti-inflammatory activity	25:50	anti-inflammatory activity	25:50	Chemical composition and anti-inflammatory activity of water extract from black cocoa tea (Camellia ptilophylla).
36192963	6	84	theme	total	775:779	arg1	theaflavins					781:791	theaflavins	781:791	theaflavins	781:791	For tea pigments, BYWE contained a little more total theaflavins and theabrownins than BCWE, while no significant difference was observed in thearubigins.
36192963	3	85	from	tea	449:451	arg1	extracts					423:430	water extracts	417:430	water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE)	417:485	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	85	from	tea	449:451	arg1	composition					371:381	chemical composition	362:381	chemical composition	362:381	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	85	from	tea	449:451	arg1	activity					405:412	anti-inflammatory activity	387:412	anti-inflammatory activity	387:412	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	86	theme	Yunnan	464:469	arg1	tea					476:478	Yunnan Daye tea	464:478	Yunnan Daye tea (BYWE)	464:485	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	3	86	theme	Yunnan	464:469	arg1	BYWE					481:484	BYWE	481:484	BYWE	481:484	In this study, the chemical composition and anti-inflammatory activity of water extracts from black cocoa tea (BCWE) and Yunnan Daye tea (BYWE) were compared.
36192963	2	87	theme	underlying	295:304	arg1	mechanisms					306:315	its underlying mechanisms	291:315	its underlying mechanisms	291:315	The anti-inflammatory activity of black cocoa tea and its underlying mechanisms remain virtually unknown.
36192963	1	88	theme	gallocatechin	208:220	arg1	gallate					222:228	high gallocatechin gallate	203:228	high gallocatechin gallate (GCG)	203:234	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	1	88	theme	gallocatechin	208:220	arg1	GCG					231:233	GCG	231:233	GCG	231:233	Cocoa tea (Camellia ptilophylla) is a non-conventional tea variety with low caffeine and high gallocatechin gallate (GCG).
36192963	8	89	theme	nitric	1067:1072	arg1	oxide					1074:1078	nitric oxide	1067:1078	nitric oxide	1067:1078	In lipopolysaccharide-induced RAW264.7 cells, both BCWE and BYWE could inhibit the production of nitric oxide and prostaglandin E2 by down-regulating the expression levels of inducible nitric oxide synthase and cyclooxygenase-2.
34611866	1	0	theme	N-linked	142:149	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	0	theme	N-linked	142:149	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	0	theme	N-linked	142:149	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	0	theme	N-linked	142:149	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	8	1	theme	MALDI-TOF	1407:1415	arg1	data					1437:1440	multiple MALDI-TOF glycomics profiling data	1398:1440	multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG)	1398:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	2	2	theme	Glycobiology	461:472	arg1	field					444:448	the field	440:448	the field of Systems Glycobiology	440:472	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	8	3	theme	Functional	1484:1493	arg1	CFG					1506:1508	CFG	1506:1508	CFG	1506:1508	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	8	3	theme	Functional	1484:1493	arg1	Glycomics					1495:1503	Functional Glycomics	1484:1503	Functional Glycomics (CFG)	1484:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	6	4	theme	such	842:845	arg1	work					847:850	such work	842:850	such work	842:850	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	1	5	theme	glycans	151:157	arg1	distribution					126:137	the distribution	122:137	the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources	122:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	6	theme	various	252:258	arg1	cell					260:263	various cell and tissue sources	252:282	cell	260:263	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	7	7	theme	cGlyco	1206:1211	arg1	usage					1197:1201	usage	1197:1201	usage	1197:1201	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	7	7	theme	cGlyco	1206:1211	arg1	development					1181:1191	development	1181:1191	development	1181:1191	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	7	8	theme	mass	1310:1313	arg1	spectrometry					1315:1326	mass spectrometry	1310:1326	multiple mass spectrometry runs	1301:1331	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	2	9	theme	glycomics	342:350	arg1	understanding					314:326	our understanding	310:326	our understanding of functional glycomics	310:350	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	1	10	theme	Glycan	58:63	arg1	strategy					87:94	a common strategy	78:94	a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources	78:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	10	theme	Glycan	58:63	arg1	profiling					65:73	Glycan profiling	58:73	Glycan profiling	58:73	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	11	theme	cell	260:263	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	11	theme	cell	260:263	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	11	theme	cell	260:263	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	11	theme	cell	260:263	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	7	12	theme	current	1151:1157	arg1	chapter					1159:1165	The current chapter	1147:1165	The current chapter	1147:1165	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	4	13	theme	liquid	699:704	arg1	spectrometry					726:737	liquid chromatography/mass spectrometry	699:737	liquid chromatography/mass spectrometry	699:737	They can also be functionalized with aglycones like 2-aminobenzamide (2AB) and procainamide to enhance separation and improve ionization during liquid chromatography/mass spectrometry.
34611866	7	14	from	runs	1328:1331	arg1	data					1291:1294	data	1291:1294	data from multiple mass spectrometry runs	1291:1331	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	6	15	from	glycans	874:880	arg1	sample					894:899	a single sample	885:899	a single sample	885:899	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	5	16	theme	released	760:767	arg1	glycans					769:775	these released glycans	754:775	these released glycans	754:775	Additionally, these released glycans may be permethylated in order to improve glycan quantitation.
34611866	2	17	theme	pathway	393:399	arg1	construction					401:412	pathway construction	393:412	pathway construction	393:412	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	6	18	theme	epitopes	1051:1058	arg1	terms					1033:1037	terms	1033:1037	terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways	1033:1144	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	5	19	theme	glycan	818:823	arg1	quantitation					825:836	glycan quantitation	818:836	glycan quantitation	818:836	Additionally, these released glycans may be permethylated in order to improve glycan quantitation.
34611866	2	20	theme	Systems	453:459	arg1	Glycobiology					461:472	Systems Glycobiology	453:472	Systems Glycobiology	453:472	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	1	21	theme	O-linked	160:167	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	21	theme	O-linked	160:167	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	21	theme	O-linked	160:167	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	21	theme	O-linked	160:167	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	0	22	theme	Glycomics	12:20	arg1	Analysis					22:29	Comparative Glycomics Analysis	0:29	Comparative Glycomics Analysis of Mass Spectrometry Data.	0:56	Comparative Glycomics Analysis of Mass Spectrometry Data.
34611866	6	23	theme	single	887:892	arg1	sample					894:899	a single sample	885:899	a single sample	885:899	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	7	24	theme	comparative	1215:1225	arg1	cGlyco					1206:1211	cGlyco	1206:1211	cGlyco ("comparative Glycomics")	1206:1237	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	7	24	theme	comparative	1215:1225	arg1	"					1236:1236	"comparative Glycomics"	1214:1236	"comparative Glycomics"	1214:1236	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	6	25	theme	underlying	977:986	arg1	similarities					988:999	similarities	988:999	similarities	988:999	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	1	26	theme	common	80:85	arg1	strategy					87:94	a common strategy	78:94	a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources	78:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	26	theme	common	80:85	arg1	profiling					65:73	Glycan profiling	58:73	Glycan profiling	58:73	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	27	theme	glycans	169:175	arg1	distribution					126:137	the distribution	122:137	the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources	122:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	0	28	theme	Comparative	0:10	arg1	Analysis					22:29	Comparative Glycomics Analysis	0:29	Comparative Glycomics Analysis of Mass Spectrometry Data.	0:56	Comparative Glycomics Analysis of Mass Spectrometry Data.
34611866	1	29	theme	tissue	269:274	arg1	sources					276:282	various cell and tissue sources	252:282	sources	276:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	30	link	O-linked	160:167	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	30	link	O-linked	160:167	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	30	link	O-linked	160:167	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	30	link	O-linked	160:167	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	7	31	used	used	1275:1278	arg2	program					1255:1261	an open-source program	1240:1261	an open-source program that can be used to compare data from multiple mass spectrometry runs	1240:1331	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	7	31	used	used	1275:1278	arg2	cGlyco					1206:1211	cGlyco	1206:1211	cGlyco ("comparative Glycomics")	1206:1237	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	8	32	theme	glycan	1380:1385	arg1	profile					1387:1393	the glycan profile	1376:1393	the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG)	1376:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	7	33	theme	multiple	1301:1308	arg1	runs					1328:1331	multiple mass spectrometry runs	1301:1331	multiple mass spectrometry runs	1301:1331	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	2	34	theme	other	418:422	arg1	applications					424:435	other applications	418:435	other applications	418:435	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	1	35	theme	sources	276:282	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	35	theme	sources	276:282	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	35	theme	sources	276:282	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	35	theme	sources	276:282	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	36	link	N-linked	142:149	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	36	link	N-linked	142:149	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	36	link	N-linked	142:149	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	36	link	N-linked	142:149	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	37	theme	glycolipid	181:190	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	37	theme	glycolipid	181:190	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	37	theme	glycolipid	181:190	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	37	theme	glycolipid	181:190	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	8	38	theme	glycomics	1417:1425	arg1	data					1437:1440	multiple MALDI-TOF glycomics profiling data	1398:1440	multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG)	1398:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	1	39	used	used	104:107	arg2	strategy					87:94	a common strategy	78:94	a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources	78:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	39	used	used	104:107	arg2	profiling					65:73	Glycan profiling	58:73	Glycan profiling	58:73	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	40	theme	associated	192:201	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	40	theme	associated	192:201	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	40	theme	associated	192:201	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	40	theme	associated	192:201	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	0	41	theme	Spectrometry	39:50	arg1	Data					52:55	Mass Spectrometry Data	34:55	Mass Spectrometry Data	34:55	Comparative Glycomics Analysis of Mass Spectrometry Data.
34611866	6	42	theme	different	1127:1135	arg1	pathways					1137:1144	different pathways	1127:1144	different pathways	1127:1144	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	6	43	theme	specific	1042:1049	arg1	epitopes					1051:1058	specific epitopes	1042:1058	specific epitopes that are changed when cells of the same origin differentiate along different pathways	1042:1144	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	7	44	dep	development	1181:1191	arg1	the					1177:1179	the	1177:1179	the	1177:1179	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	1	45	theme	complex	203:209	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	45	theme	complex	203:209	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	45	theme	complex	203:209	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	45	theme	complex	203:209	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	0	46	theme	Mass	34:37	arg1	Data					52:55	Mass Spectrometry Data	34:55	Mass Spectrometry Data	34:55	Comparative Glycomics Analysis of Mass Spectrometry Data.
34611866	8	47	theme	data	1437:1440	arg1	profile					1387:1393	the glycan profile	1376:1393	the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG)	1376:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	2	48	used	used	384:387	arg2	knowledge					362:370	this knowledge	357:370	this knowledge	357:370	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	1	49	theme	carbohydrate	211:222	arg1	glycans					169:175	O-linked glycans	160:175	O-linked glycans	160:175	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	49	theme	carbohydrate	211:222	arg1	structures					224:233	glycolipid associated complex carbohydrate structures	181:233	glycolipid associated complex carbohydrate structures	181:233	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	49	theme	carbohydrate	211:222	arg1	part					244:247	part	244:247	part of various cell and tissue sources	244:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	1	49	theme	carbohydrate	211:222	arg1	glycans					151:157	N-linked glycans	142:157	N-linked glycans	142:157	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	8	50	theme	profiling	1427:1435	arg1	data					1437:1440	multiple MALDI-TOF glycomics profiling data	1398:1440	multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG)	1398:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	2	51	theme	functional	331:340	arg1	glycomics					342:350	functional glycomics	331:350	functional glycomics	331:350	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	0	52	theme	Data	52:55	arg1	Analysis					22:29	Comparative Glycomics Analysis	0:29	Comparative Glycomics Analysis of Mass Spectrometry Data.	0:56	Comparative Glycomics Analysis of Mass Spectrometry Data.
34611866	6	53	theme	multiple	938:945	arg1	samples					947:953	multiple samples in order to determine underlying similarities and differences	938:1015	multiple samples in order to determine underlying similarities and differences	938:1015	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	3	54	theme	cell/tissue	497:507	arg1	samples					509:515	cell/tissue samples	497:515	cell/tissue samples	497:515	Glycans released from cell/tissue samples are often studied in their free-form.
34611866	4	55	theme	chromatography/mass	706:724	arg1	spectrometry					726:737	liquid chromatography/mass spectrometry	699:737	liquid chromatography/mass spectrometry	699:737	They can also be functionalized with aglycones like 2-aminobenzamide (2AB) and procainamide to enhance separation and improve ionization during liquid chromatography/mass spectrometry.
34611866	1	56	theme	structures	224:233	arg1	distribution					126:137	the distribution	122:137	the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources	122:282	Glycan profiling is a common strategy that is used to determine the distribution of N-linked glycans, O-linked glycans and glycolipid associated complex carbohydrate structures that are part of various cell and tissue sources.
34611866	2	57	theme	Such	285:288	arg1	data					290:293	Such data	285:293	Such data	285:293	Such data are central to our understanding of functional glycomics, and this knowledge can also be used for pathway construction and other applications in the field of Systems Glycobiology.
34611866	3	58	attach	released	483:490	arg1	samples					509:515	cell/tissue samples	497:515	cell/tissue samples	497:515	Glycans released from cell/tissue samples are often studied in their free-form.
34611866	3	58	attach	released	483:490	arg2	Glycans					475:481	Glycans	475:481	Glycans released from cell/tissue samples	475:515	Glycans released from cell/tissue samples are often studied in their free-form.
34611866	7	59	theme	open-source	1243:1253	arg1	cGlyco					1206:1211	cGlyco	1206:1211	cGlyco ("comparative Glycomics")	1206:1237	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	7	59	theme	open-source	1243:1253	arg1	program					1255:1261	an open-source program	1240:1261	an open-source program that can be used to compare data from multiple mass spectrometry runs	1240:1331	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	7	60	theme	Glycomics	1227:1235	arg1	cGlyco					1206:1211	cGlyco	1206:1211	cGlyco ("comparative Glycomics")	1206:1237	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	7	60	theme	Glycomics	1227:1235	arg1	"					1236:1236	"comparative Glycomics"	1214:1236	"comparative Glycomics"	1214:1236	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	8	61	theme	multiple	1398:1405	arg1	data					1437:1440	multiple MALDI-TOF glycomics profiling data	1398:1440	multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG)	1398:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	6	62	theme	origin	1100:1105	arg1	cells					1082:1086	cells	1082:1086	cells of the same origin	1082:1105	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
34611866	7	63	theme	spectrometry	1315:1326	arg1	runs					1328:1331	multiple mass spectrometry runs	1301:1331	multiple mass spectrometry runs	1301:1331	The current chapter describes the development and usage of cGlyco ("comparative Glycomics"), an open-source program that can be used to compare data from multiple mass spectrometry runs.
34611866	8	64	theme	Consortium	1469:1478	arg1	core-C					1455:1460	core-C	1455:1460	core-C of the Consortium for Functional Glycomics (CFG)	1455:1509	As an example, we apply cGlyco to compare the glycan profile of multiple MALDI-TOF glycomics profiling data collected by core-C of the Consortium for Functional Glycomics (CFG).
34611866	6	65	theme	same	1095:1098	arg1	origin					1100:1105	the same origin	1091:1105	the same origin	1091:1105	In such work, besides studying the glycans in a single sample, there is also interest in comparing multiple samples in order to determine underlying similarities and differences, for example in terms of specific epitopes that are changed when cells of the same origin differentiate along different pathways.
35337113	6	0	theme	available	993:1001	arg1	standards					1013:1021	other available reference standards	987:1021	other available reference standards	987:1021	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	5	1	theme	other	807:811	arg1	NISTmAb					844:850	NISTmAb	844:850	NISTmAb	844:850	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	5	1	theme	other	807:811	arg1	standards					833:841	other available reference standards	807:841	other available reference standards (NISTmAb)	807:851	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	1	2	theme	monoclonal	150:159	arg1	antibodies					161:170	pharmaceutical monoclonal antibodies	135:170	pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition	135:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	6	3	theme	glycan	871:876	arg1	profile					878:884	The glycan profile	867:884	The glycan profile of the USP monoclonal antibody reference standards	867:935	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	4	4	theme	USP	595:597	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	4	theme	USP	595:597	arg1	RS					607:608	USP mAb 002 RS	595:608	USP mAb 002 RS	595:608	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	5	theme	reference	552:560	arg1	RS					627:628	USP mAb 003 RS	615:628	USP mAb 003 RS	615:628	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	5	theme	reference	552:560	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	5	theme	reference	552:560	arg1	RS					607:608	USP mAb 002 RS	595:608	USP mAb 002 RS	595:608	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	5	theme	reference	552:560	arg1	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	2	6	theme	glycan	354:359	arg1	testing					369:375	glycan profile testing	354:375	glycan profile testing	354:375	Monoclonal antibody reference standards are useful as system suitability samples for glycan profile testing.
35337113	2	7	theme	suitability	330:340	arg1	samples					342:348	system suitability samples	323:348	system suitability samples for glycan profile testing	323:375	Monoclonal antibody reference standards are useful as system suitability samples for glycan profile testing.
35337113	7	8	theme	methods	1173:1179	arg1	suitability					1147:1157	the system suitability	1136:1157	the system suitability of analytical methods	1136:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	4	9	theme	antibody	543:550	arg1	RS					627:628	USP mAb 003 RS	615:628	USP mAb 003 RS	615:628	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	9	theme	antibody	543:550	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	9	theme	antibody	543:550	arg1	RS					607:608	USP mAb 002 RS	595:608	USP mAb 002 RS	595:608	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	9	theme	antibody	543:550	arg1	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	2	10	theme	reference	289:297	arg1	standards					299:307	Monoclonal antibody reference standards	269:307	Monoclonal antibody reference standards	269:307	Monoclonal antibody reference standards are useful as system suitability samples for glycan profile testing.
35337113	1	11	theme	drug	255:258	arg1	product					260:266	a drug product	253:266	a drug product	253:266	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	7	12	theme	analytical	1162:1171	arg1	methods					1173:1179	analytical methods	1162:1179	analytical methods	1162:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	4	13	dep	RS	591:592	arg1	i.e.					573:576	i.e.	573:576	i.e.	573:576	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	14	theme	monoclonal	725:734	arg1	profiles					752:759	monoclonal antibody glycan profiles	725:759	monoclonal antibody glycan profiles	725:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	2	15	theme	antibody	280:287	arg1	standards					299:307	Monoclonal antibody reference standards	269:307	Monoclonal antibody reference standards	269:307	Monoclonal antibody reference standards are useful as system suitability samples for glycan profile testing.
35337113	4	16	theme	mAb	599:601	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	16	theme	mAb	599:601	arg1	RS					607:608	USP mAb 002 RS	595:608	USP mAb 002 RS	595:608	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	5	17	theme	available	813:821	arg1	NISTmAb					844:850	NISTmAb	844:850	NISTmAb	844:850	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	5	17	theme	available	813:821	arg1	standards					833:841	other available reference standards	807:841	other available reference standards (NISTmAb)	807:851	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	4	18	used	used	684:687	arg2	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	18	used	used	684:687	arg2	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	18	used	used	684:687	arg2	RS					607:608	USP mAb 002 RS	595:608	USP mAb 002 RS	595:608	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	18	used	used	684:687	arg2	RS					627:628	USP mAb 003 RS	615:628	USP mAb 003 RS	615:628	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	2	19	theme	Monoclonal	269:278	arg1	standards					299:307	Monoclonal antibody reference standards	269:307	Monoclonal antibody reference standards	269:307	Monoclonal antibody reference standards are useful as system suitability samples for glycan profile testing.
35337113	6	20	theme	glycan	955:960	arg1	species					962:968	glycan species	955:968	glycan species	955:968	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	5	21	theme	USP	780:782	arg1	standards					794:802	the USP reference standards	776:802	the USP reference standards	776:802	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	3	22	theme	future	397:402	arg1	techniques					421:430	future glycan profiling techniques	397:430	future glycan profiling techniques	397:430	The development of future glycan profiling techniques could be better evaluated by testing well-characterized reference standards.
35337113	0	23	theme	Reference	4:12	arg1	Antibodies					34:43	USP Reference Standard Monoclonal Antibodies	0:43	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.	0:78	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.
35337113	7	24	theme	valuable	1062:1069	arg1	standards					1046:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	7	24	theme	valuable	1062:1069	arg1	tool					1071:1074	a valuable tool	1060:1074	a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods	1060:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	7	25	used	used	1088:1091	arg2	standards					1046:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	7	25	used	used	1088:1091	arg2	tool					1071:1074	a valuable tool	1060:1074	a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods	1060:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	4	26	theme	analytical	703:712	arg1	testing					714:720	the analytical testing	699:720	the analytical testing of monoclonal antibody glycan profiles	699:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	7	27	theme	mAb	1032:1034	arg1	standards					1046:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	7	27	theme	mAb	1032:1034	arg1	tool					1071:1074	a valuable tool	1060:1074	a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods	1060:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	0	28	theme	USP	0:2	arg1	Antibodies					34:43	USP Reference Standard Monoclonal Antibodies	0:43	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.	0:78	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.
35337113	4	29	theme	mAb	583:585	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	29	theme	mAb	583:585	arg1	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	30	dep	standards	562:570	arg1	RS					627:628	USP mAb 003 RS	615:628	USP mAb 003 RS	615:628	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	30	dep	standards	562:570	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	30	dep	standards	562:570	arg1	RS					607:608	USP mAb 002 RS	595:608	USP mAb 002 RS	595:608	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	30	dep	standards	562:570	arg1	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	1	31	theme	glycan	84:89	arg1	attribute					121:129	a critical quality attribute	102:129	a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition	102:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	1	31	theme	glycan	84:89	arg1	profile					91:97	The glycan profile	80:97	The glycan profile	80:97	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	1	32	theme	potential	183:191	arg1	impact					207:212	the potential physiological impact	179:212	the potential physiological impact of the glycan composition	179:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	0	33	theme	Monoclonal	23:32	arg1	Antibodies					34:43	USP Reference Standard Monoclonal Antibodies	0:43	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.	0:78	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.
35337113	4	34	theme	USP	579:581	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	34	theme	USP	579:581	arg1	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	1	35	theme	physiological	193:205	arg1	impact					207:212	the potential physiological impact	179:212	the potential physiological impact of the glycan composition	179:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	0	36	theme	Standard	14:21	arg1	Antibodies					34:43	USP Reference Standard Monoclonal Antibodies	0:43	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.	0:78	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.
35337113	4	37	theme	profiles	752:759	arg1	testing					714:720	the analytical testing	699:720	the analytical testing of monoclonal antibody glycan profiles	699:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	38	with	standards	562:570	arg1	profiles					647:654	the glycan profiles	636:654	the glycan profiles reported herein	636:670	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	39	theme	mAb	619:621	arg1	RS					627:628	USP mAb 003 RS	615:628	USP mAb 003 RS	615:628	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	39	theme	mAb	619:621	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	3	40	theme	techniques	421:430	arg1	development					382:392	The development	378:392	The development of future glycan profiling techniques	378:430	The development of future glycan profiling techniques could be better evaluated by testing well-characterized reference standards.
35337113	4	41	theme	001	587:589	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	41	theme	001	587:589	arg1	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	7	42	theme	reference	1036:1044	arg1	standards					1046:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	7	42	theme	reference	1036:1044	arg1	tool					1071:1074	a valuable tool	1060:1074	a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods	1060:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	4	43	theme	glycan	640:645	arg1	profiles					647:654	the glycan profiles	636:654	the glycan profiles reported herein	636:670	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	5	44	theme	reference	823:831	arg1	NISTmAb					844:850	NISTmAb	844:850	NISTmAb	844:850	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	5	44	theme	reference	823:831	arg1	standards					833:841	other available reference standards	807:841	other available reference standards (NISTmAb)	807:851	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	6	45	theme	standards	927:935	arg1	profile					878:884	The glycan profile	867:884	The glycan profile of the USP monoclonal antibody reference standards	867:935	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	5	46	theme	standards	794:802	arg1	Comparison					762:771	Comparison	762:771	Comparison of the USP reference standards to other available reference standards (NISTmAb)	762:851	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
35337113	3	47	theme	well-characterized	469:486	arg1	standards					498:506	well-characterized reference standards	469:506	well-characterized reference standards	469:506	The development of future glycan profiling techniques could be better evaluated by testing well-characterized reference standards.
35337113	3	48	theme	glycan	404:409	arg1	techniques					421:430	future glycan profiling techniques	397:430	future glycan profiling techniques	397:430	The development of future glycan profiling techniques could be better evaluated by testing well-characterized reference standards.
35337113	6	49	theme	reference	917:925	arg1	standards					927:935	the USP monoclonal antibody reference standards	889:935	the USP monoclonal antibody reference standards	889:935	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	7	50	theme	USP	1028:1030	arg1	standards					1046:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards	1024:1054	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	7	50	theme	USP	1028:1030	arg1	tool					1071:1074	a valuable tool	1060:1074	a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods	1060:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	3	51	theme	profiling	411:419	arg1	techniques					421:430	future glycan profiling techniques	397:430	future glycan profiling techniques	397:430	The development of future glycan profiling techniques could be better evaluated by testing well-characterized reference standards.
35337113	6	52	theme	antibody	908:915	arg1	standards					927:935	the USP monoclonal antibody reference standards	889:935	the USP monoclonal antibody reference standards	889:935	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	6	53	theme	USP	893:895	arg1	antibody					908:915	USP monoclonal antibody	893:915	the USP monoclonal antibody reference standards	889:935	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	1	54	theme	critical	104:111	arg1	attribute					121:129	a critical quality attribute	102:129	a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition	102:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	1	54	theme	critical	104:111	arg1	profile					91:97	The glycan profile	80:97	The glycan profile	80:97	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	6	55	theme	monoclonal	897:906	arg1	antibody					908:915	USP monoclonal antibody	893:915	the USP monoclonal antibody reference standards	889:935	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	7	56	theme	system	1140:1145	arg1	suitability					1147:1157	the system suitability	1136:1157	the system suitability of analytical methods	1136:1179	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	7	57	theme	glycan	1107:1112	arg1	structure					1114:1122	the glycan structure	1103:1122	the glycan structure	1103:1122	The USP mAb reference standards are a valuable tool that can be used to verify the glycan structure and provide the system suitability of analytical methods.
35337113	6	58	theme	reference	1003:1011	arg1	standards					1013:1021	other available reference standards	987:1021	other available reference standards	987:1021	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	1	59	theme	quality	113:119	arg1	attribute					121:129	a critical quality attribute	102:129	a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition	102:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	1	59	theme	quality	113:119	arg1	profile					91:97	The glycan profile	80:97	The glycan profile	80:97	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	1	60	theme	glycan	221:226	arg1	composition					228:238	the glycan composition	217:238	the glycan composition	217:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	4	61	theme	glycan	745:750	arg1	profiles					752:759	monoclonal antibody glycan profiles	725:759	monoclonal antibody glycan profiles	725:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	62	theme	antibody	736:743	arg1	profiles					752:759	monoclonal antibody glycan profiles	725:759	monoclonal antibody glycan profiles	725:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	2	63	theme	system	323:328	arg1	samples					342:348	system suitability samples	323:348	system suitability samples for glycan profile testing	323:375	Monoclonal antibody reference standards are useful as system suitability samples for glycan profile testing.
35337113	1	64	theme	composition	228:238	arg1	impact					207:212	the potential physiological impact	179:212	the potential physiological impact of the glycan composition	179:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	0	65	theme	Glycan	62:67	arg1	Structure					69:77	Glycan Structure	62:77	Glycan Structure	62:77	USP Reference Standard Monoclonal Antibodies: Tools to Verify Glycan Structure.
35337113	3	66	theme	reference	488:496	arg1	standards					498:506	well-characterized reference standards	469:506	well-characterized reference standards	469:506	The development of future glycan profiling techniques could be better evaluated by testing well-characterized reference standards.
35337113	2	67	theme	profile	361:367	arg1	testing					369:375	glycan profile testing	354:375	glycan profile testing	354:375	Monoclonal antibody reference standards are useful as system suitability samples for glycan profile testing.
35337113	4	68	theme	USP	615:617	arg1	RS					627:628	USP mAb 003 RS	615:628	USP mAb 003 RS	615:628	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	68	theme	USP	615:617	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	6	69	theme	species	962:968	arg1	range					946:950	a range	944:950	a range of glycan species that complements other available reference standards	944:1021	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	4	70	theme	monoclonal	532:541	arg1	RS					627:628	USP mAb 003 RS	615:628	USP mAb 003 RS	615:628	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	70	theme	monoclonal	532:541	arg1	standards					562:570	monoclonal antibody reference standards	532:570	monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles	532:759	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	70	theme	monoclonal	532:541	arg1	RS					607:608	USP mAb 002 RS	595:608	USP mAb 002 RS	595:608	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	4	70	theme	monoclonal	532:541	arg1	RS					591:592	USP mAb 001 RS	579:592	USP mAb 001 RS	579:592	The USP has introduced monoclonal antibody reference standards (i.e., USP mAb 001 RS, USP mAb 002 RS, and USP mAb 003 RS) with the glycan profiles reported herein that can be used to assess the analytical testing of monoclonal antibody glycan profiles.
35337113	6	71	theme	other	987:991	arg1	standards					1013:1021	other available reference standards	987:1021	other available reference standards	987:1021	The glycan profile of the USP monoclonal antibody reference standards covers a range of glycan species that complements other available reference standards.
35337113	1	72	theme	pharmaceutical	135:148	arg1	antibodies					161:170	pharmaceutical monoclonal antibodies	135:170	pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition	135:238	The glycan profile is a critical quality attribute for pharmaceutical monoclonal antibodies due to the potential physiological impact of the glycan composition when used as a drug product.
35337113	5	73	theme	reference	784:792	arg1	standards					794:802	the USP reference standards	776:802	the USP reference standards	776:802	Comparison of the USP reference standards to other available reference standards (NISTmAb) is presented.
36044512	9	0	theme	preservation	1193:1204	arg1	solutions					1206:1214	preservation solutions	1193:1214	preservation solutions	1193:1214	Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72.
36044512	13	1	theme	renal	1740:1744	arg1	tissue					1746:1751	renal tissue	1740:1751	renal tissue	1740:1751	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	12	2	theme	modified	1584:1591	arg1	mUW					1597:1599	mUW	1597:1599	mUW	1597:1599	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	12	2	theme	modified	1584:1591	arg1	UW					1593:1594	modified UW	1584:1594	modified UW (mUW)	1584:1600	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	13	3	dep	72	1763:1764	arg1	to					1760:1761	to	1760:1761	to	1760:1761	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	4	4	theme	intensive	420:428	arg1	efforts					430:436	intensive efforts	420:436	intensive efforts	420:436	Despite intensive efforts, the functional preservation of solid organs prior to transplantation is limited to hours.
36044512	12	5	from	low	1577:1579	arg1	groups					1620:1625	mUW-g groups	1614:1625	mUW-g groups	1614:1625	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	12	5	from	low	1577:1579	arg1	mUW					1597:1599	mUW	1597:1599	mUW	1597:1599	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	12	5	from	low	1577:1579	arg1	mUW-m					1603:1607	mUW-m	1603:1607	mUW-m	1603:1607	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	12	5	from	low	1577:1579	arg1	UW					1593:1594	modified UW	1584:1594	modified UW (mUW)	1584:1600	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	0	6	from	Melatonin	0:8	arg1	solutions					26:34	preservation solutions	13:34	preservation solutions	13:34	Melatonin in preservation solutions prevents ischemic injury in rat kidneys.
36044512	5	7	theme	ischemic	681:688	arg1	injury					690:695	ischemic injury	681:695	ischemic injury	681:695	In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury.
36044512	8	8	theme	biochemical	1032:1042	arg1	analysis					1062:1069	biochemical and microscopical analysis	1032:1069	biochemical and microscopical analysis	1032:1069	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	10	9	theme	modified	1338:1345	arg1	groups					1404:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	9	10	theme	dehydrogenase	1161:1173	arg1	activity					1181:1188	Lactate dehydrogenase (LDH) activity	1153:1188	Lactate dehydrogenase (LDH) activity in preservation solutions	1153:1214	Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72.
36044512	5	11	theme	HTK	615:617	arg1	solutions					619:627	both the UW and HTK solutions	599:627	solutions	619:627	In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury.
36044512	10	12	theme	preservation	1283:1294	arg1	h					1278:1278	2 h	1276:1278	2 h of preservation in all groups	1276:1308	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	12	theme	preservation	1283:1294	arg1	h					1317:1317	10 h	1314:1317	10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1314:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	13	13	theme	effective	1697:1705	arg1	solution					1662:1669	The mUW-m solution	1652:1669	The mUW-m solution at low temperature	1652:1688	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	13	13	theme	effective	1697:1705	arg1	solution					1720:1727	an effective and suitable solution	1694:1727	an effective and suitable solution to protect renal tissue for up to 72 h	1694:1766	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	0	14	from	injury	54:59	arg1	kidneys					68:74	rat kidneys	64:74	rat kidneys	64:74	Melatonin in preservation solutions prevents ischemic injury in rat kidneys.
36044512	6	15	theme	main	839:842	arg1	component					844:852	the main component	835:852	the main component of HTK solution	835:868	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	6	15	theme	main	839:842	arg1	Histidine/Histidine-HCl					802:824	Histidine/Histidine-HCl	802:824	Histidine/Histidine-HCl which is the main component of HTK solution	802:868	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	6	16	theme	solution	724:731	arg1	composition					702:712	The composition	698:712	The composition of the UW solution	698:731	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	6	17	theme	HTK	857:859	arg1	solution					861:868	HTK solution	857:868	HTK solution	857:868	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	11	18	dep	activity	1445:1452	arg1	0.63-1.17					1466:1474	0.63-1.17	1466:1474	0.63-1.17	1466:1474	At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group.
36044512	11	18	dep	activity	1445:1452	arg1	IU/g					1460:1463	0.91 IU/g	1455:1463	0.91 IU/g (0.63-1.17)	1455:1475	At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group.
36044512	6	19	theme	UW	721:722	arg1	solution					724:731	the UW solution	717:731	the UW solution	717:731	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	10	20	from	groups	1303:1308	arg1	h					1278:1278	2 h	1276:1278	2 h of preservation in all groups	1276:1308	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	20	from	groups	1303:1308	arg1	h					1317:1317	10 h	1314:1317	10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1314:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	13	21	theme	low	1674:1676	arg1	temperature					1678:1688	low temperature	1674:1688	low temperature	1674:1688	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	10	22	theme	UW+glucosamine	1381:1394	arg1	groups					1404:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	7	23	theme	preservation	889:900	arg1	solutions					902:910	the preservation solutions	885:910	the preservation solutions	885:910	Additionally, the preservation solutions were supplemented with melatonin and glucosamine.
36044512	10	24	from	h	1317:1317	arg1	groups					1404:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	24	from	h	1317:1317	arg1	groups					1303:1308	all groups	1299:1308	all groups	1299:1308	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	24	from	h	1317:1317	arg1	detectable					1262:1271	detectable	1262:1271	detectable	1262:1271	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	25	from	h	1278:1278	arg1	groups					1404:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	25	from	h	1278:1278	arg1	groups					1303:1308	all groups	1299:1308	all groups	1299:1308	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	25	from	h	1278:1278	arg1	detectable					1262:1271	detectable	1262:1271	detectable	1262:1271	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	5	26	theme	tissue-protective	648:664	arg1	effect					666:671	their tissue-protective effect	642:671	their tissue-protective effect against ischemic injury	642:695	In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury.
36044512	10	27	theme	modified	1372:1379	arg1	mUW-g					1397:1401	mUW-g	1397:1401	mUW-g	1397:1401	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	27	theme	modified	1372:1379	arg1	UW+glucosamine					1381:1394	modified UW+glucosamine	1372:1394	modified UW+glucosamine (mUW-g)	1372:1402	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	11	28	theme	72nd	1419:1422	arg1	hour					1424:1427	the 72nd hour	1415:1427	the 72nd hour	1415:1427	At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group.
36044512	9	29	from	activity	1181:1188	arg1	solutions					1206:1214	preservation solutions	1193:1214	preservation solutions	1193:1214	Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72.
36044512	8	30	theme	rat	1115:1117	arg1	kidneys					1119:1125	the rat kidneys	1111:1125	the rat kidneys with static cold storage	1111:1150	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	8	31	theme	microscopical	1048:1060	arg1	analysis					1062:1069	biochemical and microscopical analysis	1032:1069	biochemical and microscopical analysis	1032:1069	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	5	32	theme	UW	608:609	arg1	solutions					619:627	both the UW and HTK solutions	599:627	solutions	619:627	In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury.
36044512	10	33	from	preservation	1322:1333	arg1	groups					1404:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	3	34	theme	preservation	388:399	arg1	solutions					401:409	preservation solutions	388:409	preservation solutions	388:409	Currently, the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) are the most commonly used preservation solutions.
36044512	12	35	theme	UW	1532:1533	arg1	group					1535:1539	the UW group	1528:1539	the UW group	1528:1539	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	3	36	used	used	383:386	arg2	University					287:296	University	287:296	University	287:296	Currently, the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) are the most commonly used preservation solutions.
36044512	4	37	theme	organs	476:481	arg1	preservation					454:465	the functional preservation	439:465	the functional preservation of solid organs prior to transplantation	439:506	Despite intensive efforts, the functional preservation of solid organs prior to transplantation is limited to hours.
36044512	12	38	theme	mUW-g	1614:1618	arg1	groups					1620:1625	mUW-g groups	1614:1625	mUW-g groups	1614:1625	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	8	39	theme	protective	966:975	arg1	effects					977:983	The protective effects	962:983	The protective effects of the preservation solutions	962:1013	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	12	40	from	groups	1620:1625	arg1	low					1577:1579	low	1577:1579	low	1577:1579	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	0	41	theme	ischemic	45:52	arg1	injury					54:59	ischemic injury	45:59	ischemic injury in rat kidneys	45:74	Melatonin in preservation solutions prevents ischemic injury in rat kidneys.
36044512	10	42	from	groups	1404:1409	arg1	h					1278:1278	2 h	1276:1278	2 h of preservation in all groups	1276:1308	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	42	from	groups	1404:1409	arg1	h					1317:1317	10 h	1314:1317	10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1314:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	4	43	theme	solid	470:474	arg1	organs					476:481	solid organs	470:481	solid organs	470:481	Despite intensive efforts, the functional preservation of solid organs prior to transplantation is limited to hours.
36044512	12	44	theme	histopathological	1542:1558	arg1	damage					1560:1565	histopathological damage	1542:1565	histopathological damage score	1542:1571	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	2	45	theme	transplantation	173:187	arg1	success					189:195	The transplantation success	169:195	The transplantation success	169:195	The transplantation success depends on the functional preservation of organs prior to transplantation.
36044512	2	46	theme	prior	246:250	arg1	organs					239:244	organs	239:244	organs prior to transplantation	239:269	The transplantation success depends on the functional preservation of organs prior to transplantation.
36044512	9	47	theme	Lactate	1153:1159	arg1	LDH					1176:1178	LDH	1176:1178	LDH	1176:1178	Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72.
36044512	9	47	theme	Lactate	1153:1159	arg1	dehydrogenase					1161:1173	Lactate dehydrogenase	1153:1173	Lactate dehydrogenase (LDH) activity in preservation solutions	1153:1214	Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72.
36044512	12	48	theme	damage	1560:1565	arg1	score					1567:1571	histopathological damage score	1542:1571	histopathological damage score	1542:1571	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	8	49	theme	cold	1139:1142	arg1	storage					1144:1150	static cold storage	1132:1150	static cold storage	1132:1150	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	13	50	theme	mUW-m	1656:1660	arg1	solution					1662:1669	The mUW-m solution	1652:1669	The mUW-m solution at low temperature	1652:1688	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	13	50	theme	mUW-m	1656:1660	arg1	solution					1720:1727	an effective and suitable solution	1694:1727	an effective and suitable solution to protect renal tissue for up to 72 h	1694:1766	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	2	51	theme	organs	239:244	arg1	preservation					223:234	the functional preservation	208:234	the functional preservation of organs prior to transplantation	208:269	The transplantation success depends on the functional preservation of organs prior to transplantation.
36044512	5	52	theme	UW	560:561	arg1	solution					563:570	the UW solution	556:570	the UW solution containing components from both the UW and HTK solutions	556:627	In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury.
36044512	5	53	contain	containing	572:581	arg2	components					583:592	components	583:592	components	583:592	In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury.
36044512	5	53	contain	containing	572:581	arg1	solution					563:570	the UW solution	556:570	the UW solution containing components from both the UW and HTK solutions	556:627	In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury.
36044512	8	54	theme	static	1132:1137	arg1	storage					1144:1150	static cold storage	1132:1150	static cold storage	1132:1150	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	4	55	theme	functional	443:452	arg1	preservation					454:465	the functional preservation	439:465	the functional preservation of solid organs prior to transplantation	439:506	Despite intensive efforts, the functional preservation of solid organs prior to transplantation is limited to hours.
36044512	13	56	from	temperature	1678:1688	arg1	solution					1662:1669	The mUW-m solution	1652:1669	The mUW-m solution at low temperature	1652:1688	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	13	56	from	temperature	1678:1688	arg1	solution					1720:1727	an effective and suitable solution	1694:1727	an effective and suitable solution to protect renal tissue for up to 72 h	1694:1766	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	8	57	theme	preservation	992:1003	arg1	solutions					1005:1013	the preservation solutions	988:1013	the preservation solutions	988:1013	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	1	58	theme	effective	120:128	arg1	treatment					130:138	the most effective treatment	111:138	the most effective treatment	111:138	Transplantation is lifesaving and the most effective treatment for end-stage organ failure.
36044512	11	59	theme	lowest	1434:1439	arg1	activity					1445:1452	the lowest LDH activity	1430:1452	the lowest LDH activity (0.91 IU/g (0.63-1.17))	1430:1476	At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group.
36044512	12	60	from	mUW-m	1603:1607	arg1	low					1577:1579	low	1577:1579	low	1577:1579	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	2	61	theme	functional	212:221	arg1	preservation					223:234	the functional preservation	208:234	the functional preservation of organs prior to transplantation	208:269	The transplantation success depends on the functional preservation of organs prior to transplantation.
36044512	10	62	from	preservation	1283:1294	arg1	groups					1303:1308	all groups	1299:1308	all groups	1299:1308	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	6	63	theme	starch	770:775	arg1	concentration					777:789	hydroxyethyl starch concentration	757:789	hydroxyethyl starch concentration	757:789	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	11	64	theme	mUW-m	1498:1502	arg1	group					1504:1508	the mUW-m group	1494:1508	the mUW-m group	1494:1508	At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group.
36044512	8	65	with	kidneys	1119:1125	arg1	storage					1144:1150	static cold storage	1132:1150	static cold storage	1132:1150	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	12	66	dep	group	1535:1539	arg1	comparison					1514:1523	comparison	1514:1523	comparison	1514:1523	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	6	67	theme	hydroxyethyl	757:768	arg1	starch					770:775	hydroxyethyl starch	757:775	hydroxyethyl starch concentration	757:789	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	12	68	from	hours	1645:1649	arg1	low					1577:1579	low	1577:1579	low	1577:1579	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	13	69	theme	suitable	1711:1718	arg1	solution					1662:1669	The mUW-m solution	1652:1669	The mUW-m solution at low temperature	1652:1688	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	13	69	theme	suitable	1711:1718	arg1	solution					1720:1727	an effective and suitable solution	1694:1727	an effective and suitable solution to protect renal tissue for up to 72 h	1694:1766	The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.
36044512	6	70	theme	solution	861:868	arg1	component					844:852	the main component	835:852	the main component of HTK solution	835:868	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	6	70	theme	solution	861:868	arg1	Histidine/Histidine-HCl					802:824	Histidine/Histidine-HCl	802:824	Histidine/Histidine-HCl which is the main component of HTK solution	802:868	The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCl which is the main component of HTK solution.
36044512	12	71	from	UW	1593:1594	arg1	low					1577:1579	low	1577:1579	low	1577:1579	In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours.
36044512	8	72	theme	solutions	1005:1013	arg1	effects					977:983	The protective effects	962:983	The protective effects of the preservation solutions	962:1013	The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage.
36044512	1	73	theme	end-stage	144:152	arg1	failure					160:166	end-stage organ failure	144:166	end-stage organ failure	144:166	Transplantation is lifesaving and the most effective treatment for end-stage organ failure.
36044512	11	74	theme	LDH	1441:1443	arg1	activity					1445:1452	the lowest LDH activity	1430:1452	the lowest LDH activity (0.91 IU/g (0.63-1.17))	1430:1476	At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group.
36044512	10	75	theme	UW+melatonin	1347:1358	arg1	groups					1404:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1338:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	0	76	theme	rat	64:66	arg1	kidneys					68:74	rat kidneys	64:74	rat kidneys	64:74	Melatonin in preservation solutions prevents ischemic injury in rat kidneys.
36044512	0	77	theme	preservation	13:24	arg1	solutions					26:34	preservation solutions	13:34	preservation solutions	13:34	Melatonin in preservation solutions prevents ischemic injury in rat kidneys.
36044512	10	78	theme	preservation	1322:1333	arg1	h					1278:1278	2 h	1276:1278	2 h of preservation in all groups	1276:1308	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	10	78	theme	preservation	1322:1333	arg1	h					1317:1317	10 h	1314:1317	10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups	1314:1409	It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups.
36044512	1	79	theme	organ	154:158	arg1	failure					160:166	end-stage organ failure	144:166	end-stage organ failure	144:166	Transplantation is lifesaving and the most effective treatment for end-stage organ failure.
35929524	0	0	theme	intestinal	72:81	arg1	microbiota					83:92	intestinal microbiota	72:92	intestinal microbiota	72:92	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	1	1	theme	alcoholic	294:302	arg1	injury					310:315	alcoholic liver injury	294:315	alcoholic liver injury	294:315	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	2	2	dep	%	426:426	arg1	v/v					429:431	v/v	429:431	v/v	429:431	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	4	3	theme	rhamnosus	799:807	arg1	B10					809:811	L. rhamnosus B10	796:811	L. rhamnosus B10	796:811	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	1	4	theme	L.	154:155	arg1	B10					167:169	L. rhamnosus B10	154:169	L. rhamnosus B10	154:169	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	1	4	theme	L.	154:155	arg1	B10					149:151	Lactobacillus rhamnosus B10	125:151	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces	125:199	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	4	5	theme	12.5 mmol/g	946:956	arg1	aminotransferase					928:943	aspartate aminotransferase	918:943	aspartate aminotransferase (12.5 mmol/g prot)	918:962	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	5	theme	12.5 mmol/g	946:956	arg1	prot					958:961	12.5 mmol/g prot	946:961	12.5 mmol/g prot	946:961	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	1	6	theme	mice	225:228	arg1	model					230:234	an alcohol mice model	214:234	an alcohol mice model	214:234	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	4	7	theme	alanine	870:876	arg1	prot					908:911	17.8 mmol/g prot	896:911	17.8 mmol/g prot	896:911	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	7	theme	alanine	870:876	arg1	triglycerides					836:848	the liver triglycerides	826:848	the liver triglycerides (1.02 mmol/g prot)	826:867	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	7	theme	alanine	870:876	arg1	aminotransferase					878:893	alanine aminotransferase	870:893	alanine aminotransferase (17.8 mmol/g prot)	870:912	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	0	8	from	effect	4:9	arg1	injury					61:66	alcoholic liver injury	45:66	alcoholic liver injury	45:66	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	0	8	from	effect	4:9	arg1	microbiota					83:92	intestinal microbiota	72:92	intestinal microbiota	72:92	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	6	9	theme	symbiotic	1153:1161	arg1	abundance					1163:1171	The intestinal symbiotic abundance	1138:1171	The intestinal symbiotic abundance of Firmicutes	1138:1185	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	1	10	theme	rhamnosus	157:165	arg1	B10					167:169	L. rhamnosus B10	154:169	L. rhamnosus B10	154:169	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	1	10	theme	rhamnosus	157:165	arg1	B10					149:151	Lactobacillus rhamnosus B10	125:151	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces	125:199	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	3	11	theme	tumor	746:750	arg1	necrosis					752:759	tumor necrosis factor-α	746:768	tumor necrosis factor-α (138 pg/mL)	746:780	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	3	11	theme	tumor	746:750	arg1	138 pg/mL					771:779	138 pg/mL	771:779	138 pg/mL	771:779	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	7	12	theme	intestinal	1460:1469	arg1	flora					1471:1475	intestinal flora	1460:1475	intestinal flora	1460:1475	In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.
35929524	5	13	from	changes	1036:1042	arg1	cecum					1117:1121	cecum	1117:1121	cecum	1117:1121	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	5	13	from	changes	1036:1042	arg1	family					1091:1096	family	1091:1096	family	1091:1096	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	5	13	from	changes	1036:1042	arg1	level					1108:1112	genus level	1102:1112	genus level	1102:1112	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	5	13	from	changes	1036:1042	arg1	class					1084:1088	class	1084:1088	class	1084:1088	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	6	14	theme	Firmicutes	1176:1185	arg1	abundance					1163:1171	The intestinal symbiotic abundance	1138:1171	The intestinal symbiotic abundance of Firmicutes	1138:1185	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	7	15	theme	liver	1433:1437	arg1	injury					1439:1444	alcoholic liver injury	1423:1444	alcoholic liver injury	1423:1444	In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.
35929524	0	16	theme	mice	113:116	arg1	model					118:122	alcohol-induced mice model	97:122	alcohol-induced mice model	97:122	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	7	17	theme	therapeutic	1386:1396	arg1	effects					1398:1404	the therapeutic effects	1382:1404	the therapeutic effects of probiotics on alcoholic liver injury	1382:1444	In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.
35929524	5	18	from	family	1091:1096	arg1	changes					1036:1042	The changes	1032:1042	The changes of intestinal microbiota composition on class, family and genus level in cecum	1032:1121	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	2	19	theme	liquid	382:387	arg1	Lieber-DeCarli					394:407	liquid diet Lieber-DeCarli	382:407	liquid diet Lieber-DeCarli	382:407	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	6	20	theme	rhamnosus	1310:1318	arg1	B10					1320:1322	L. rhamnosus B10	1307:1322	L. rhamnosus B10	1307:1322	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	0	21	theme	alcohol-induced	97:111	arg1	model					118:122	alcohol-induced mice model	97:122	alcohol-induced mice model	97:122	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	1	22	theme	intestinal	331:340	arg1	microbiota					342:351	intestinal microbiota	331:351	intestinal microbiota	331:351	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	1	23	from	effects	263:269	arg1	injury					310:315	alcoholic liver injury	294:315	alcoholic liver injury	294:315	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	4	24	theme	liver	830:834	arg1	triglycerides					836:848	the liver triglycerides	826:848	the liver triglycerides (1.02 mmol/g prot)	826:867	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	24	theme	liver	830:834	arg1	prot					863:866	1.02 mmol/g prot	851:866	1.02 mmol/g prot	851:866	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	24	theme	liver	830:834	arg1	aminotransferase					928:943	aspartate aminotransferase	918:943	aspartate aminotransferase (12.5 mmol/g prot)	918:962	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	24	theme	liver	830:834	arg1	aminotransferase					878:893	alanine aminotransferase	870:893	alanine aminotransferase (17.8 mmol/g prot)	870:912	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	5	25	theme	composition	1069:1079	arg1	changes					1036:1042	The changes	1032:1042	The changes of intestinal microbiota composition on class, family and genus level in cecum	1032:1121	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	4	26	theme	aspartate	918:926	arg1	triglycerides					836:848	the liver triglycerides	826:848	the liver triglycerides (1.02 mmol/g prot)	826:867	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	26	theme	aspartate	918:926	arg1	aminotransferase					928:943	aspartate aminotransferase	918:943	aspartate aminotransferase (12.5 mmol/g prot)	918:962	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	26	theme	aspartate	918:926	arg1	prot					958:961	12.5 mmol/g prot	946:961	12.5 mmol/g prot	946:961	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	3	27	theme	alanine	633:639	arg1	26.4 U/L					659:666	26.4 U/L	659:666	26.4 U/L	659:666	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	3	27	theme	alanine	633:639	arg1	aminotransferase					641:656	alanine aminotransferase	633:656	alanine aminotransferase (26.4 U/L)	633:667	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	5	28	from	class	1084:1088	arg1	changes					1036:1042	The changes	1032:1042	The changes of intestinal microbiota composition on class, family and genus level in cecum	1032:1121	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	6	29	theme	gram-negative	1206:1218	arg1	Proteobacteria					1229:1242	Proteobacteria	1229:1242	Proteobacteria	1229:1242	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	6	29	theme	gram-negative	1206:1218	arg1	bacteria					1220:1227	gram-negative bacteria	1206:1227	gram-negative bacteria Proteobacteria and Deferribacteres	1206:1262	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	6	29	theme	gram-negative	1206:1218	arg1	Deferribacteres					1248:1262	Deferribacteres	1248:1262	Deferribacteres	1248:1262	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	2	30	theme	C57BL/6N	354:361	arg1	mice					363:366	C57BL/6N mice	354:366	C57BL/6N mice	354:366	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	1	31	theme	liver	304:308	arg1	injury					310:315	alcoholic liver injury	294:315	alcoholic liver injury	294:315	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	5	32	theme	genus	1102:1106	arg1	level					1108:1112	genus level	1102:1112	genus level	1102:1112	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	6	33	theme	intestinal	1142:1151	arg1	abundance					1163:1171	The intestinal symbiotic abundance	1138:1171	The intestinal symbiotic abundance of Firmicutes	1138:1185	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	4	34	theme	alcohol-induced	1002:1016	arg1	injury					1024:1029	alcohol-induced liver injury	1002:1029	alcohol-induced liver injury	1002:1029	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	0	35	theme	Lactobacillus	14:26	arg1	B10					38:40	Lactobacillus rhamnosus B10	14:40	Lactobacillus rhamnosus B10	14:40	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	3	36	theme	aspartate	670:678	arg1	14.2 U/L					698:705	14.2 U/L	698:705	14.2 U/L	698:705	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	3	36	theme	aspartate	670:678	arg1	aminotransferase					680:695	aspartate aminotransferase	670:695	aspartate aminotransferase (14.2 U/L)	670:706	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	6	37	theme	alcohol	1281:1287	arg1	mice					1289:1292	alcohol mice	1281:1292	alcohol mice treated with L. rhamnosus B10 for 2 weeks	1281:1334	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	6	38	theme	L.	1307:1308	arg1	B10					1320:1322	L. rhamnosus B10	1307:1322	L. rhamnosus B10	1307:1322	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	2	39	theme	diet	389:392	arg1	Lieber-DeCarli					394:407	liquid diet Lieber-DeCarli	382:407	liquid diet Lieber-DeCarli	382:407	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	3	40	theme	total	571:575	arg1	1.48 mmol/L					590:600	1.48 mmol/L	590:600	1.48 mmol/L	590:600	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	3	40	theme	total	571:575	arg1	cholesterol					577:587	the serum total cholesterol	561:587	the serum total cholesterol (1.48 mmol/L)	561:601	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	2	41	theme	%	426:426	arg1	ethanol					434:440	5% (v/v) ethanol	425:440	5% (v/v) ethanol for 8 weeks	425:452	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	1	42	theme	baby	190:193	arg1	feces					195:199	the baby feces	186:199	the baby feces	186:199	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	0	43	theme	B10	38:40	arg1	effect					4:9	The effect	0:9	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.	0:123	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	2	44	theme	rhamnosus	475:483	arg1	B10					485:487	L. rhamnosus B10	472:487	L. rhamnosus B10	472:487	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	5	45	from	composition	1069:1079	arg1	family					1091:1096	family	1091:1096	family	1091:1096	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	5	45	from	composition	1069:1079	arg1	level					1108:1112	genus level	1102:1112	genus level	1102:1112	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	5	45	from	composition	1069:1079	arg1	class					1084:1088	class	1084:1088	class	1084:1088	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	4	46	theme	liver	1018:1022	arg1	injury					1024:1029	alcohol-induced liver injury	1002:1029	alcohol-induced liver injury	1002:1029	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	7	47	from	effects	1398:1404	arg1	injury					1439:1444	alcoholic liver injury	1423:1444	alcoholic liver injury	1423:1444	In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.
35929524	0	48	theme	rhamnosus	28:36	arg1	B10					38:40	Lactobacillus rhamnosus B10	14:40	Lactobacillus rhamnosus B10	14:40	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	5	49	theme	intestinal	1047:1056	arg1	composition					1069:1079	intestinal microbiota composition	1047:1079	intestinal microbiota composition on class, family and genus level	1047:1112	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	2	50	theme	L.	472:473	arg1	B10					485:487	L. rhamnosus B10	472:487	L. rhamnosus B10	472:487	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	0	51	from	injury	61:66	arg1	model					118:122	alcohol-induced mice model	97:122	alcohol-induced mice model	97:122	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	4	52	theme	1.02 mmol/g	851:861	arg1	triglycerides					836:848	the liver triglycerides	826:848	the liver triglycerides (1.02 mmol/g prot)	826:867	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	52	theme	1.02 mmol/g	851:861	arg1	prot					863:866	1.02 mmol/g prot	851:866	1.02 mmol/g prot	851:866	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	0	53	theme	alcoholic	45:53	arg1	injury					61:66	alcoholic liver injury	45:66	alcoholic liver injury	45:66	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	1	54	theme	L.	274:275	arg1	B10					287:289	L. rhamnosus B10	274:289	L. rhamnosus B10	274:289	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	4	55	theme	L.	796:797	arg1	B10					809:811	L. rhamnosus B10	796:811	L. rhamnosus B10	796:811	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	1	56	attach	isolated	172:179	arg2	B10					167:169	L. rhamnosus B10	154:169	L. rhamnosus B10	154:169	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	1	56	attach	isolated	172:179	arg1	feces					195:199	the baby feces	186:199	the baby feces	186:199	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	1	56	attach	isolated	172:179	arg2	B10					149:151	Lactobacillus rhamnosus B10	125:151	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces	125:199	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	2	57	theme	5	425:425	arg1	%					426:426	%	426:426	%	426:426	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	1	58	theme	rhamnosus	277:285	arg1	B10					287:289	L. rhamnosus B10	274:289	L. rhamnosus B10	274:289	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	7	59	theme	probiotics	1409:1418	arg1	effects					1398:1404	the therapeutic effects	1382:1404	the therapeutic effects of probiotics on alcoholic liver injury	1382:1444	In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.
35929524	3	60	theme	serum	565:569	arg1	1.48 mmol/L					590:600	1.48 mmol/L	590:600	1.48 mmol/L	590:600	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	3	60	theme	serum	565:569	arg1	cholesterol					577:587	the serum total cholesterol	561:587	the serum total cholesterol (1.48 mmol/L)	561:601	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	1	61	theme	B10	287:289	arg1	effects					263:269	the effects	259:269	the effects of L. rhamnosus B10 on alcoholic liver injury	259:315	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	2	62	theme	last	496:499	arg1	2 weeks					501:507	the last 2 weeks	492:507	the last 2 weeks	492:507	C57BL/6N mice were fed with liquid diet Lieber-DeCarli with or without 5% (v/v) ethanol for 8 weeks, and treated with L. rhamnosus B10 at the last 2 weeks.
35929524	1	63	theme	Lactobacillus	125:137	arg1	B10					167:169	L. rhamnosus B10	154:169	L. rhamnosus B10	154:169	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	1	63	theme	Lactobacillus	125:137	arg1	B10					149:151	Lactobacillus rhamnosus B10	125:151	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces	125:199	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	4	64	theme	alcohol	967:973	arg1	mice					975:978	alcohol mice	967:978	alcohol mice	967:978	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	5	65	from	level	1108:1112	arg1	changes					1036:1042	The changes	1032:1042	The changes of intestinal microbiota composition on class, family and genus level in cecum	1032:1121	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	4	66	theme	17.8 mmol/g	896:906	arg1	prot					908:911	17.8 mmol/g prot	896:911	17.8 mmol/g prot	896:911	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	4	66	theme	17.8 mmol/g	896:906	arg1	aminotransferase					878:893	alanine aminotransferase	870:893	alanine aminotransferase (17.8 mmol/g prot)	870:912	In addition, L. rhamnosus B10 also reduced the liver triglycerides (1.02 mmol/g prot), alanine aminotransferase (17.8 mmol/g prot) and aspartate aminotransferase (12.5 mmol/g prot) in alcohol mice, thereby ameliorating alcohol-induced liver injury.
35929524	7	67	theme	alcoholic	1423:1431	arg1	injury					1439:1444	alcoholic liver injury	1423:1444	alcoholic liver injury	1423:1444	In summary, this study provided evidence for the therapeutic effects of probiotics on alcoholic liver injury by regulating intestinal flora.
35929524	1	68	theme	rhamnosus	139:147	arg1	B10					167:169	L. rhamnosus B10	154:169	L. rhamnosus B10	154:169	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	1	68	theme	rhamnosus	139:147	arg1	B10					149:151	Lactobacillus rhamnosus B10	125:151	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces	125:199	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
35929524	0	69	from	microbiota	83:92	arg1	model					118:122	alcohol-induced mice model	97:122	alcohol-induced mice model	97:122	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	3	70	theme	L.	534:535	arg1	B10					547:549	L. rhamnosus B10	534:549	L. rhamnosus B10	534:549	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	0	71	theme	liver	55:59	arg1	injury					61:66	alcoholic liver injury	45:66	alcoholic liver injury	45:66	The effect of Lactobacillus rhamnosus B10 on alcoholic liver injury and intestinal microbiota in alcohol-induced mice model.
35929524	5	72	theme	microbiota	1058:1067	arg1	composition					1069:1079	intestinal microbiota composition	1047:1079	intestinal microbiota composition on class, family and genus level	1047:1112	The changes of intestinal microbiota composition on class, family and genus level in cecum were analyzed.
35929524	3	73	theme	rhamnosus	537:545	arg1	B10					547:549	L. rhamnosus B10	534:549	L. rhamnosus B10	534:549	The results showed that L. rhamnosus B10 decreased the serum total cholesterol (1.48 mmol/L), triglycerides (0.97 mmol/L), alanine aminotransferase (26.4 U/L), aspartate aminotransferase (14.2 U/L), lipopolysaccharide (0.23 EU/mL), and tumor necrosis factor-α (138 pg/mL).
35929524	6	74	dep	bacteria	1220:1227	arg1	Proteobacteria					1229:1242	Proteobacteria	1229:1242	Proteobacteria	1229:1242	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	6	74	dep	bacteria	1220:1227	arg1	bacteria					1220:1227	gram-negative bacteria	1206:1227	gram-negative bacteria Proteobacteria and Deferribacteres	1206:1262	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	6	74	dep	bacteria	1220:1227	arg1	Deferribacteres					1248:1262	Deferribacteres	1248:1262	Deferribacteres	1248:1262	The intestinal symbiotic abundance of Firmicutes was elevated while gram-negative bacteria Proteobacteria and Deferribacteres was decreased in alcohol mice treated with L. rhamnosus B10 for 2 weeks.
35929524	1	75	theme	alcohol	217:223	arg1	model					230:234	an alcohol mice model	214:234	an alcohol mice model	214:234	Lactobacillus rhamnosus B10 (L. rhamnosus B10) isolated from the baby feces was given to an alcohol mice model, aiming to investigate the effects of L. rhamnosus B10 on alcoholic liver injury by regulating intestinal microbiota.
36794950	7	0	from	increase	765:772	arg1	LPS					929:931	LPS	929:931	LPS	929:931	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	0	from	increase	765:772	arg1	lipopolysaccharide					909:926	lipopolysaccharide	909:926	lipopolysaccharide (LPS)	909:932	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	0	from	increase	765:772	arg1	bacteria					788:795	beneficial bacteria	777:795	beneficial bacteria (e.g., Rikenella and Alistipes)	777:827	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	0	from	increase	765:772	arg1	bacteria					867:874	potentially harmful bacteria	847:874	potentially harmful bacteria (e.g., Escherichia-Shigella)	847:903	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	1	theme	harmful	859:865	arg1	bacteria					867:874	potentially harmful bacteria	847:874	potentially harmful bacteria (e.g., Escherichia-Shigella)	847:903	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	2	dep	Rikenella	804:812	arg1	e.g.					798:801	e.g.	798:801	e.g.	798:801	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	9	3	theme	Phylogenetic	1212:1223	arg1	Investigation					1225:1237	Phylogenetic Investigation	1212:1237	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt)	1212:1301	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	7	4	theme	gut	731:733	arg1	dysbiosis					735:743	gut dysbiosis	731:743	gut dysbiosis	731:743	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	15	5	theme	side	2290:2293	arg1	effects					2295:2301	side effects	2290:2301	side effects	2290:2301	This Syn is as effective as prednisone in treating AIH without side effects.
36794950	8	6	theme	intestinal	993:1002	arg1	integrity					1012:1020	intestinal barrier integrity	993:1020	intestinal barrier integrity	993:1020	The Syn maintained intestinal barrier integrity, reduced LPS, and inhibited the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway.
36794950	1	7	theme	liver	189:193	arg1	hepatitis					168:176	Autoimmune hepatitis	157:176	Autoimmune hepatitis (AIH)	157:182	Autoimmune hepatitis (AIH) is a liver disease characterized by chronic liver inflammation.
36794950	1	7	theme	liver	189:193	arg1	disease					195:201	a liver disease	187:201	a liver disease characterized by chronic liver inflammation	187:245	Autoimmune hepatitis (AIH) is a liver disease characterized by chronic liver inflammation.
36794950	13	8	theme	new	1840:1842	arg1	Syn					1844:1846	our new Syn	1836:1846	our new Syn	1836:1846	Our data indicate that our new Syn not only reverses gut dysbiosis by increasing beneficial bacteria and decreasing lipopolysaccharide (LPS)-bearing Gram-negative bacteria but also maintains intestinal barrier integrity.
36794950	0	9	theme	TLR4/NF-κB/NLRP3	121:136	arg1	Pathway					148:154	the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway	109:154	the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway	109:154	A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway.
36794950	2	10	theme	AIH	309:311	arg1	progression					313:323	AIH progression	309:323	AIH progression	309:323	The intestinal barrier and microbiome play critical roles in AIH progression.
36794950	9	11	theme	States	1286:1291	arg1	Reconstruction					1257:1270	Reconstruction	1257:1270	Reconstruction of Unobserved States (PICRUSt)	1257:1301	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	5	12	theme	AIH	560:562	arg1	model					570:574	an AIH mouse model	557:574	an AIH mouse model	557:574	This study investigated the effects of a novel synbiotic in an AIH mouse model.
36794950	1	13	theme	chronic	220:226	arg1	inflammation					234:245	chronic liver inflammation	220:245	chronic liver inflammation	220:245	Autoimmune hepatitis (AIH) is a liver disease characterized by chronic liver inflammation.
36794950	4	14	theme	synbiotic	476:484	arg1	therapies					486:494	synbiotic therapies	476:494	synbiotic therapies	476:494	Thus, there is growing interest in developing synbiotic therapies.
36794950	12	15	theme	hepatic	1776:1782	arg1	inflammation					1784:1795	inflammation	1784:1795	inflammation	1784:1795	IMPORTANCE Synbiotics can ameliorate liver injury and improve liver function by reducing hepatic inflammation and pyroptosis.
36794950	13	16	theme	barrier	2015:2021	arg1	integrity					2023:2031	intestinal barrier integrity	2004:2031	intestinal barrier integrity	2004:2031	Our data indicate that our new Syn not only reverses gut dysbiosis by increasing beneficial bacteria and decreasing lipopolysaccharide (LPS)-bearing Gram-negative bacteria but also maintains intestinal barrier integrity.
36794950	5	17	theme	novel	538:542	arg1	synbiotic					544:552	a novel synbiotic	536:552	a novel synbiotic	536:552	This study investigated the effects of a novel synbiotic in an AIH mouse model.
36794950	16	18	theme	clinical	2396:2403	arg1	practice					2405:2412	clinical practice	2396:2412	clinical practice	2396:2412	Based on these findings, this novel Syn represents a potential therapeutic agent for AIH in clinical practice.
36794950	2	19	theme	intestinal	252:261	arg1	barrier					263:269	The intestinal barrier	248:269	The intestinal barrier	248:269	The intestinal barrier and microbiome play critical roles in AIH progression.
36794950	12	20	theme	liver	1749:1753	arg1	function					1755:1762	liver function	1749:1762	liver function	1749:1762	IMPORTANCE Synbiotics can ameliorate liver injury and improve liver function by reducing hepatic inflammation and pyroptosis.
36794950	13	21	theme	Gram-negative	1962:1974	arg1	bacteria					1976:1983	lipopolysaccharide (LPS)-bearing Gram-negative bacteria	1929:1983	lipopolysaccharide (LPS)-bearing Gram-negative bacteria	1929:1983	Our data indicate that our new Syn not only reverses gut dysbiosis by increasing beneficial bacteria and decreasing lipopolysaccharide (LPS)-bearing Gram-negative bacteria but also maintains intestinal barrier integrity.
36794950	0	22	theme	Microbiota-Liver	72:87	arg1	Axis					89:92	the Gut Microbiota-Liver Axis	64:92	the Gut Microbiota-Liver Axis	64:92	A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway.
36794950	9	23	theme	gut	1328:1330	arg1	function					1343:1350	gut microbiota function	1328:1350	gut microbiota function	1328:1350	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	7	24	theme	bacterial	956:964	arg1	levels					966:971	-bearing Gram-negative bacterial levels	933:971	-bearing Gram-negative bacterial levels	933:971	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	25	theme	-bearing	933:940	arg1	levels					966:971	-bearing Gram-negative bacterial levels	933:971	-bearing Gram-negative bacterial levels	933:971	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	12	26	theme	liver	1724:1728	arg1	injury					1730:1735	liver injury	1724:1735	liver injury	1724:1735	IMPORTANCE Synbiotics can ameliorate liver injury and improve liver function by reducing hepatic inflammation and pyroptosis.
36794950	3	27	theme	first-line	368:377	arg1	drugs					379:383	first-line drugs	368:383	first-line drugs	368:383	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	6	28	theme	liver	650:654	arg1	function					656:663	liver function	650:663	liver function	650:663	We found that this synbiotic (Syn) ameliorated liver injury and improved liver function by reducing hepatic inflammation and pyroptosis.
36794950	9	29	theme	inflammatory	1362:1373	arg1	injury					1375:1380	inflammatory injury	1362:1380	inflammatory injury	1362:1380	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	14	30	theme	microbiota	2094:2103	arg1	composition					2105:2115	gut microbiota composition	2090:2115	gut microbiota composition	2090:2115	Thus, its mechanism might be associated with modulating gut microbiota composition and intestinal barrier function by inhibiting the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver.
36794950	3	31	theme	many	411:414	arg1	effects					421:427	many side effects	411:427	many side effects	411:427	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	8	32	theme	TLR4/NF-κB	1054:1063	arg1	pathway					1095:1101	the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway	1050:1101	the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway	1050:1101	The Syn maintained intestinal barrier integrity, reduced LPS, and inhibited the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway.
36794950	12	33	theme	IMPORTANCE	1687:1696	arg1	Synbiotics					1698:1707	IMPORTANCE Synbiotics	1687:1707	IMPORTANCE Synbiotics	1687:1707	IMPORTANCE Synbiotics can ameliorate liver injury and improve liver function by reducing hepatic inflammation and pyroptosis.
36794950	16	34	theme	potential	2357:2365	arg1	agent					2379:2383	a potential therapeutic agent	2355:2383	a potential therapeutic agent for AIH in clinical practice	2355:2412	Based on these findings, this novel Syn represents a potential therapeutic agent for AIH in clinical practice.
36794950	8	35	theme	NLRP3/Caspase-1	1069:1083	arg1	pathway					1095:1101	the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway	1050:1101	the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway	1050:1101	The Syn maintained intestinal barrier integrity, reduced LPS, and inhibited the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway.
36794950	14	36	theme	TLR4/NF-κB/NLRP3/pyroptosis	2167:2193	arg1	pathway					2205:2211	the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway	2163:2211	the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver	2163:2224	Thus, its mechanism might be associated with modulating gut microbiota composition and intestinal barrier function by inhibiting the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver.
36794950	9	37	theme	microbiome	1117:1126	arg1	prediction					1138:1147	microbiome phenotype prediction	1117:1147	microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt)	1117:1301	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	0	38	theme	Autoimmune	29:38	arg1	Hepatitis					40:48	Autoimmune Hepatitis	29:48	Autoimmune Hepatitis	29:48	A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway.
36794950	7	39	theme	beneficial	777:786	arg1	bacteria					788:795	beneficial bacteria	777:795	beneficial bacteria (e.g., Rikenella and Alistipes)	777:827	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	14	40	from	pathway	2205:2211	arg1	liver					2220:2224	the liver	2216:2224	the liver	2216:2224	Thus, its mechanism might be associated with modulating gut microbiota composition and intestinal barrier function by inhibiting the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver.
36794950	11	41	theme	endothelial	1644:1654	arg1	dysfunction					1656:1666	endothelial dysfunction	1644:1666	endothelial dysfunction	1644:1666	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	1	42	theme	liver	228:232	arg1	inflammation					234:245	chronic liver inflammation	220:245	chronic liver inflammation	220:245	Autoimmune hepatitis (AIH) is a liver disease characterized by chronic liver inflammation.
36794950	16	43	from	agent	2379:2383	arg1	practice					2405:2412	clinical practice	2396:2412	clinical practice	2396:2412	Based on these findings, this novel Syn represents a potential therapeutic agent for AIH in clinical practice.
36794950	13	44	theme	gut	1866:1868	arg1	dysbiosis					1870:1878	gut dysbiosis	1866:1878	gut dysbiosis	1866:1878	Our data indicate that our new Syn not only reverses gut dysbiosis by increasing beneficial bacteria and decreasing lipopolysaccharide (LPS)-bearing Gram-negative bacteria but also maintains intestinal barrier integrity.
36794950	9	45	theme	bacterial	1164:1172	arg1	prediction					1195:1204	bacterial functional potential prediction	1164:1204	bacterial functional potential prediction	1164:1204	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	5	46	from	effects	525:531	arg1	model					570:574	an AIH mouse model	557:574	an AIH mouse model	557:574	This study investigated the effects of a novel synbiotic in an AIH mouse model.
36794950	9	47	theme	potential	1185:1193	arg1	prediction					1195:1204	bacterial functional potential prediction	1164:1204	bacterial functional potential prediction	1164:1204	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	11	48	theme	novel	1515:1519	arg1	Syn					1521:1523	this novel Syn	1510:1523	this novel Syn	1510:1523	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	11	48	theme	novel	1515:1519	arg1	drug					1546:1549	a candidate drug	1534:1549	a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis	1534:1684	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	11	49	theme	antipyroptosis	1605:1618	arg1	properties					1620:1629	its anti-inflammatory and antipyroptosis properties	1579:1629	its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis	1579:1684	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	7	50	dep	Escherichia-Shigella	883:902	arg1	e.g.					877:880	e.g.	877:880	e.g.	877:880	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	51	dep	increase	765:772	arg1	levels					966:971	-bearing Gram-negative bacterial levels	933:971	-bearing Gram-negative bacterial levels	933:971	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	2	52	theme	critical	291:298	arg1	roles					300:304	critical roles	291:304	critical roles	291:304	The intestinal barrier and microbiome play critical roles in AIH progression.
36794950	8	53	theme	barrier	1004:1010	arg1	integrity					1012:1020	intestinal barrier integrity	993:1020	intestinal barrier integrity	993:1020	The Syn maintained intestinal barrier integrity, reduced LPS, and inhibited the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway.
36794950	9	54	theme	Communities	1242:1252	arg1	Investigation					1225:1237	Phylogenetic Investigation	1212:1237	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt)	1212:1301	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	16	55	dep	potential	2357:2365	arg1	therapeutic					2367:2377	therapeutic	2367:2377	therapeutic	2367:2377	Based on these findings, this novel Syn represents a potential therapeutic agent for AIH in clinical practice.
36794950	0	56	theme	Signaling	138:146	arg1	Pathway					148:154	the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway	109:154	the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway	109:154	A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway.
36794950	10	57	theme	new	1447:1449	arg1	Syn					1451:1453	the new Syn	1443:1453	the new Syn	1443:1453	Furthermore, the new Syn was as effective as prednisone against AIH.
36794950	10	57	theme	new	1447:1449	arg1	effective					1462:1470	effective	1462:1470	effective	1462:1470	Furthermore, the new Syn was as effective as prednisone against AIH.
36794950	9	58	theme	Unobserved	1275:1284	arg1	States					1286:1291	Unobserved States	1275:1291	Unobserved States (PICRUSt)	1275:1301	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	9	58	theme	Unobserved	1275:1284	arg1	PICRUSt					1294:1300	PICRUSt	1294:1300	PICRUSt	1294:1300	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	5	59	theme	mouse	564:568	arg1	model					570:574	an AIH mouse model	557:574	an AIH mouse model	557:574	This study investigated the effects of a novel synbiotic in an AIH mouse model.
36794950	3	60	theme	AIH	326:328	arg1	treatment					330:338	AIH treatment	326:338	AIH treatment	326:338	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	11	61	theme	anti-inflammatory	1583:1599	arg1	properties					1620:1629	its anti-inflammatory and antipyroptosis properties	1579:1629	its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis	1579:1684	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	13	62	theme	intestinal	2004:2013	arg1	integrity					2023:2031	intestinal barrier integrity	2004:2031	intestinal barrier integrity	2004:2031	Our data indicate that our new Syn not only reverses gut dysbiosis by increasing beneficial bacteria and decreasing lipopolysaccharide (LPS)-bearing Gram-negative bacteria but also maintains intestinal barrier integrity.
36794950	0	63	theme	Gut	68:70	arg1	Axis					89:92	the Gut Microbiota-Liver Axis	64:92	the Gut Microbiota-Liver Axis	64:92	A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway.
36794950	5	64	theme	synbiotic	544:552	arg1	effects					525:531	the effects	521:531	the effects of a novel synbiotic in an AIH mouse model	521:574	This study investigated the effects of a novel synbiotic in an AIH mouse model.
36794950	1	65	theme	Autoimmune	157:166	arg1	hepatitis					168:176	Autoimmune hepatitis	157:176	Autoimmune hepatitis (AIH)	157:182	Autoimmune hepatitis (AIH) is a liver disease characterized by chronic liver inflammation.
36794950	1	65	theme	Autoimmune	157:166	arg1	disease					195:201	a liver disease	187:201	a liver disease characterized by chronic liver inflammation	187:245	Autoimmune hepatitis (AIH) is a liver disease characterized by chronic liver inflammation.
36794950	1	65	theme	Autoimmune	157:166	arg1	AIH					179:181	AIH	179:181	AIH	179:181	Autoimmune hepatitis (AIH) is a liver disease characterized by chronic liver inflammation.
36794950	13	66	theme	-bearing	1953:1960	arg1	bacteria					1976:1983	lipopolysaccharide (LPS)-bearing Gram-negative bacteria	1929:1983	lipopolysaccharide (LPS)-bearing Gram-negative bacteria	1929:1983	Our data indicate that our new Syn not only reverses gut dysbiosis by increasing beneficial bacteria and decreasing lipopolysaccharide (LPS)-bearing Gram-negative bacteria but also maintains intestinal barrier integrity.
36794950	6	67	theme	liver	624:628	arg1	injury					630:635	liver injury	624:635	liver injury	624:635	We found that this synbiotic (Syn) ameliorated liver injury and improved liver function by reducing hepatic inflammation and pyroptosis.
36794950	9	68	theme	microbiota	1332:1341	arg1	function					1343:1350	gut microbiota function	1328:1350	gut microbiota function	1328:1350	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	14	69	theme	intestinal	2121:2130	arg1	function					2140:2147	intestinal barrier function	2121:2147	intestinal barrier function	2121:2147	Thus, its mechanism might be associated with modulating gut microbiota composition and intestinal barrier function by inhibiting the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver.
36794950	3	70	theme	limited	390:396	arg1	efficacy					398:405	limited efficacy	390:405	limited efficacy	390:405	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	0	71	theme	Hepatic	113:119	arg1	Pathway					148:154	the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway	109:154	the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway	109:154	A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway.
36794950	7	72	theme	Gram-negative	942:954	arg1	levels					966:971	-bearing Gram-negative bacterial levels	933:971	-bearing Gram-negative bacterial levels	933:971	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	11	73	theme	candidate	1536:1544	arg1	Syn					1521:1523	this novel Syn	1510:1523	this novel Syn	1510:1523	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	11	73	theme	candidate	1536:1544	arg1	drug					1546:1549	a candidate drug	1534:1549	a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis	1534:1684	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	16	74	theme	novel	2334:2338	arg1	Syn					2340:2342	this novel Syn	2329:2342	this novel Syn	2329:2342	Based on these findings, this novel Syn represents a potential therapeutic agent for AIH in clinical practice.
36794950	0	75	theme	Novel	2:6	arg1	Synbiotic					8:16	A Novel Synbiotic	0:16	A Novel Synbiotic	0:16	A Novel Synbiotic Alleviates Autoimmune Hepatitis by Modulating the Gut Microbiota-Liver Axis and Inhibiting the Hepatic TLR4/NF-κB/NLRP3 Signaling Pathway.
36794950	14	76	theme	gut	2090:2092	arg1	composition					2105:2115	gut microbiota composition	2090:2115	gut microbiota composition	2090:2115	Thus, its mechanism might be associated with modulating gut microbiota composition and intestinal barrier function by inhibiting the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver.
36794950	3	77	theme	side	416:419	arg1	effects					421:427	many side effects	411:427	many side effects	411:427	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	13	78	theme	beneficial	1894:1903	arg1	bacteria					1905:1912	beneficial bacteria	1894:1912	beneficial bacteria	1894:1912	Our data indicate that our new Syn not only reverses gut dysbiosis by increasing beneficial bacteria and decreasing lipopolysaccharide (LPS)-bearing Gram-negative bacteria but also maintains intestinal barrier integrity.
36794950	8	79	theme	signaling	1085:1093	arg1	pathway					1095:1101	the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway	1050:1101	the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway	1050:1101	The Syn maintained intestinal barrier integrity, reduced LPS, and inhibited the TLR4/NF-κB and NLRP3/Caspase-1 signaling pathway.
36794950	14	80	theme	signaling	2195:2203	arg1	pathway					2205:2211	the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway	2163:2211	the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver	2163:2224	Thus, its mechanism might be associated with modulating gut microbiota composition and intestinal barrier function by inhibiting the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver.
36794950	6	81	theme	hepatic	677:683	arg1	inflammation					685:696	inflammation	685:696	inflammation	685:696	We found that this synbiotic (Syn) ameliorated liver injury and improved liver function by reducing hepatic inflammation and pyroptosis.
36794950	9	82	theme	immune	1395:1400	arg1	response					1402:1409	immune response	1395:1409	immune response	1395:1409	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	9	83	theme	phenotype	1128:1136	arg1	prediction					1138:1147	microbiome phenotype prediction	1117:1147	microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt)	1117:1301	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36794950	3	84	contain	have	385:388	arg1	drugs					379:383	first-line drugs	368:383	first-line drugs	368:383	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	3	84	contain	have	385:388	arg2	effects					421:427	many side effects	411:427	many side effects	411:427	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	3	84	contain	have	385:388	arg2	efficacy					398:405	limited efficacy	390:405	limited efficacy	390:405	AIH treatment remains challenging because first-line drugs have limited efficacy and many side effects.
36794950	14	85	theme	barrier	2132:2138	arg1	function					2140:2147	intestinal barrier function	2121:2147	intestinal barrier function	2121:2147	Thus, its mechanism might be associated with modulating gut microbiota composition and intestinal barrier function by inhibiting the TLR4/NF-κB/NLRP3/pyroptosis signaling pathway in the liver.
36794950	7	86	from	decrease	835:842	arg1	LPS					929:931	LPS	929:931	LPS	929:931	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	86	from	decrease	835:842	arg1	lipopolysaccharide					909:926	lipopolysaccharide	909:926	lipopolysaccharide (LPS)	909:932	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	86	from	decrease	835:842	arg1	bacteria					788:795	beneficial bacteria	777:795	beneficial bacteria (e.g., Rikenella and Alistipes)	777:827	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	7	86	from	decrease	835:842	arg1	bacteria					867:874	potentially harmful bacteria	847:874	potentially harmful bacteria (e.g., Escherichia-Shigella)	847:903	The Syn reversed gut dysbiosis, as indicated by an increase in beneficial bacteria (e.g., Rikenella and Alistipes) and a decrease in potentially harmful bacteria (e.g., Escherichia-Shigella) and lipopolysaccharide (LPS)-bearing Gram-negative bacterial levels.
36794950	11	87	theme	gut	1672:1674	arg1	dysbiosis					1676:1684	gut dysbiosis	1672:1684	gut dysbiosis	1672:1684	Therefore, this novel Syn could be a candidate drug for alleviating AIH through its anti-inflammatory and antipyroptosis properties that relieve endothelial dysfunction and gut dysbiosis.
36794950	9	88	theme	functional	1174:1183	arg1	prediction					1195:1204	bacterial functional potential prediction	1164:1204	bacterial functional potential prediction	1164:1204	In addition, microbiome phenotype prediction by BugBase and bacterial functional potential prediction using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that Syn improved gut microbiota function involving inflammatory injury, metabolism, immune response, and pathopoiesia.
36049525	0	0	theme	batatas	91:97	arg1	phenotypes					127:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	6	1	theme	indirect	864:871	arg1	correlation					873:883	an indirect correlation	861:883	an indirect correlation of starch and carotenoids	861:909	The metabolite data suggested an indirect correlation of starch and carotenoids through the TCA cycle and pentose phosphate pathway.
36049525	7	2	theme	hydrophilic	1069:1079	arg1	environment					1081:1091	the hydrophilic environment	1065:1091	the hydrophilic environment of the amyloplast	1065:1109	Furthermore, a change in lipid composition was observed to accommodate the storage of carotenoids in the hydrophilic environment of the amyloplast.
36049525	2	3	theme	solutions	308:316	arg1	solutions					308:316	the biofortification solutions	287:316	the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD	287:387	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	2	3	theme	solutions	308:316	arg1	one					280:282	one	280:282	one	280:282	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	4	4	from	amyloplasts	614:624	arg1	located					603:609	located	603:609	located	603:609	Both starch and carotenoid formation are located in amyloplasts and could potentially compete for the same precursors.
36049525	0	5	theme	Ipomoea	83:89	arg1	phenotypes					127:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	1	6	from	deficiency	149:158	arg1	countries					191:199	Low and Medium Income countries	169:199	Low and Medium Income countries	169:199	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	2	7	theme	biofortification	291:306	arg1	solutions					308:316	the biofortification solutions	287:316	the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD	287:387	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	3	8	from	reduction	465:473	arg1	matter					482:487	dry matter	478:487	dry matter	478:487	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	6	9	theme	phosphate	945:953	arg1	pathway					955:961	pentose phosphate pathway	937:961	pentose phosphate pathway	937:961	The metabolite data suggested an indirect correlation of starch and carotenoids through the TCA cycle and pentose phosphate pathway.
36049525	0	10	theme	sweet	100:104	arg1	phenotypes					127:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	0	11	from	Changes	0:6	arg1	subplastidal					33:44	subplastidal	33:44	subplastidal	33:44	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	0	11	from	Changes	0:6	arg1	allocation					18:27	carbon allocation	11:27	carbon allocation	11:27	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	3	12	theme	calorific	499:507	arg1	value					509:513	calorific value	499:513	calorific value	499:513	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	8	13	from	metabolism	1193:1202	arg1	β-carotene					1212:1221	high β-carotene	1207:1221	high β-carotene producing sweet potato roots	1207:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	4	14	from	located	603:609	arg1	amyloplasts					614:624	amyloplasts	614:624	amyloplasts	614:624	Both starch and carotenoid formation are located in amyloplasts and could potentially compete for the same precursors.
36049525	6	15	theme	pentose	937:943	arg1	pathway					955:961	pentose phosphate pathway	937:961	pentose phosphate pathway	937:961	The metabolite data suggested an indirect correlation of starch and carotenoids through the TCA cycle and pentose phosphate pathway.
36049525	4	16	located	located	603:609	arg1	amyloplasts					614:624	amyloplasts	614:624	amyloplasts	614:624	Both starch and carotenoid formation are located in amyloplasts and could potentially compete for the same precursors.
36049525	4	16	located	located	603:609	arg2	formation					589:597	carotenoid formation	578:597	carotenoid formation	578:597	Both starch and carotenoid formation are located in amyloplasts and could potentially compete for the same precursors.
36049525	4	16	located	located	603:609	arg2	starch					567:572	starch	567:572	starch	567:572	Both starch and carotenoid formation are located in amyloplasts and could potentially compete for the same precursors.
36049525	7	17	theme	amyloplast	1100:1109	arg1	environment					1081:1091	the hydrophilic environment	1065:1091	the hydrophilic environment of the amyloplast	1065:1109	Furthermore, a change in lipid composition was observed to accommodate the storage of carotenoids in the hydrophilic environment of the amyloplast.
36049525	2	18	theme	sweet	257:261	arg1	OSP					271:273	OSP	271:273	OSP	271:273	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	2	18	theme	sweet	257:261	arg1	potato					263:268	orange sweet potato	250:268	orange sweet potato (OSP)	250:274	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	2	18	theme	sweet	257:261	arg1	batatas					241:247	Ipomoea batatas	233:247	Ipomoea batatas	233:247	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	9	19	theme	root	1355:1358	arg1	tissue					1360:1365	sweet potato root tissue	1342:1365	sweet potato root tissue	1342:1365	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	8	20	theme	potato	1239:1244	arg1	roots					1246:1250	sweet potato roots	1233:1250	sweet potato roots	1233:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	6	21	theme	metabolite	835:844	arg1	data					846:849	The metabolite data	831:849	The metabolite data	831:849	The metabolite data suggested an indirect correlation of starch and carotenoids through the TCA cycle and pentose phosphate pathway.
36049525	3	22	theme	provitamin	404:413	arg1	content					430:436	high provitamin A (β-carotene) content	399:436	high provitamin A (β-carotene) content	399:436	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	2	23	theme	orange	250:255	arg1	OSP					271:273	OSP	271:273	OSP	271:273	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	2	23	theme	orange	250:255	arg1	potato					263:268	orange sweet potato	250:268	orange sweet potato (OSP)	250:274	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	2	23	theme	orange	250:255	arg1	batatas					241:247	Ipomoea batatas	233:247	Ipomoea batatas	233:247	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	3	24	from	effects	534:540	arg1	traits					554:559	consumer traits	545:559	consumer traits	545:559	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	2	25	theme	Health	349:354	arg1	Organisation					356:367	the World Health Organisation (WHO)	339:373	the World Health Organisation (WHO)	339:373	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	0	26	theme	potato	106:111	arg1	phenotypes					127:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	8	27	from	perturbation	1177:1188	arg1	β-carotene					1212:1221	high β-carotene	1207:1221	high β-carotene producing sweet potato roots	1207:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	7	28	theme	carotenoids	1050:1060	arg1	storage					1039:1045	the storage	1035:1045	the storage of carotenoids	1035:1060	Furthermore, a change in lipid composition was observed to accommodate the storage of carotenoids in the hydrophilic environment of the amyloplast.
36049525	2	29	theme	World	343:347	arg1	Organisation					356:367	the World Health Organisation (WHO)	339:373	the World Health Organisation (WHO)	339:373	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	5	30	theme	different	693:701	arg1	phenotypes					729:738	five different sweet potato storage root phenotypes	688:738	five different sweet potato storage root phenotypes	688:738	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	4	31	theme	same	664:667	arg1	precursors					669:678	the same precursors	660:678	the same precursors	660:678	Both starch and carotenoid formation are located in amyloplasts and could potentially compete for the same precursors.
36049525	9	32	theme	expression	1290:1299	arg1	analysis					1301:1308	previous gene expression analysis	1276:1308	previous gene expression analysis	1276:1308	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	5	33	theme	sub-plastidal	810:822	arg1	level					824:828	the sub-plastidal level	806:828	the sub-plastidal level	806:828	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	9	34	theme	biochemical	1318:1328	arg1	analysis					1330:1337	biochemical analysis	1318:1337	biochemical analysis of sweet potato root tissue	1318:1365	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	8	35	theme	high	1207:1210	arg1	β-carotene					1212:1221	high β-carotene	1207:1221	high β-carotene producing sweet potato roots	1207:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	9	36	theme	tissue	1360:1365	arg1	analysis					1330:1337	biochemical analysis	1318:1337	biochemical analysis of sweet potato root tissue	1318:1365	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	8	37	theme	sweet	1233:1237	arg1	roots					1246:1250	sweet potato roots	1233:1250	sweet potato roots	1233:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	1	38	theme	Low	169:171	arg1	countries					191:199	Low and Medium Income countries	169:199	Low and Medium Income countries	169:199	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	8	39	theme	ultra-structures	1156:1171	arg1	perturbation					1177:1188	perturbation	1177:1188	perturbation of metabolism in high β-carotene producing sweet potato roots	1177:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	8	39	theme	ultra-structures	1156:1171	arg1	alteration					1133:1142	an alteration	1130:1142	an alteration of cellular ultra-structures	1130:1171	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	6	40	theme	TCA	923:925	arg1	cycle					927:931	the TCA cycle	919:931	the TCA cycle	919:931	The metabolite data suggested an indirect correlation of starch and carotenoids through the TCA cycle and pentose phosphate pathway.
36049525	0	41	theme	root	122:125	arg1	phenotypes					127:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	3	42	theme	dry	478:480	arg1	matter					482:487	dry matter	478:487	dry matter	478:487	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	3	43	theme	high	399:402	arg1	content					430:436	high provitamin A (β-carotene) content	399:436	high provitamin A (β-carotene) content	399:436	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	0	44	theme	carbon	11:16	arg1	allocation					18:27	carbon allocation	11:27	carbon allocation	11:27	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	0	45	theme	storage	114:120	arg1	phenotypes					127:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	8	46	from	alteration	1133:1142	arg1	β-carotene					1212:1221	high β-carotene	1207:1221	high β-carotene producing sweet potato roots	1207:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	1	47	theme	Medium	177:182	arg1	countries					191:199	Low and Medium Income countries	169:199	Low and Medium Income countries	169:199	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	5	48	theme	sweet	703:707	arg1	phenotypes					729:738	five different sweet potato storage root phenotypes	688:738	five different sweet potato storage root phenotypes	688:738	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	9	49	theme	sweet	1342:1346	arg1	tissue					1360:1365	sweet potato root tissue	1342:1365	sweet potato root tissue	1342:1365	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	8	50	theme	cellular	1147:1154	arg1	ultra-structures					1156:1171	cellular ultra-structures	1147:1171	cellular ultra-structures	1147:1171	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	1	51	theme	Income	184:189	arg1	countries					191:199	Low and Medium Income countries	169:199	Low and Medium Income countries	169:199	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	4	52	theme	carotenoid	578:587	arg1	formation					589:597	carotenoid formation	578:597	carotenoid formation	578:597	Both starch and carotenoid formation are located in amyloplasts and could potentially compete for the same precursors.
36049525	6	53	theme	carotenoids	899:909	arg1	correlation					873:883	an indirect correlation	861:883	an indirect correlation of starch and carotenoids	861:909	The metabolite data suggested an indirect correlation of starch and carotenoids through the TCA cycle and pentose phosphate pathway.
36049525	0	54	theme	phenotypes	127:136	arg1	structures					57:66	structures	57:66	structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes	57:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	9	55	theme	gene	1285:1288	arg1	analysis					1301:1308	previous gene expression analysis	1276:1308	previous gene expression analysis	1276:1308	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	2	56	dep	Organisation	356:367	arg1	WHO					370:372	WHO	370:372	WHO	370:372	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	5	57	theme	storage	716:722	arg1	phenotypes					729:738	five different sweet potato storage root phenotypes	688:738	five different sweet potato storage root phenotypes	688:738	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	9	58	theme	potato	1348:1353	arg1	tissue					1360:1365	sweet potato root tissue	1342:1365	sweet potato root tissue	1342:1365	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	7	59	theme	lipid	989:993	arg1	composition					995:1005	lipid composition	989:1005	lipid composition	989:1005	Furthermore, a change in lipid composition was observed to accommodate the storage of carotenoids in the hydrophilic environment of the amyloplast.
36049525	5	60	from	level	824:828	arg1	proteomics					792:801	proteomics	792:801	proteomics	792:801	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	5	60	from	level	824:828	arg1	metabolomics					775:786	spatial metabolomics	767:786	spatial metabolomics	767:786	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	3	61	theme	consumer	545:552	arg1	traits					554:559	consumer traits	545:559	consumer traits	545:559	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	8	62	from	β-carotene	1212:1221	arg1	perturbation					1177:1188	perturbation	1177:1188	perturbation of metabolism in high β-carotene producing sweet potato roots	1177:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	8	62	from	β-carotene	1212:1221	arg1	alteration					1133:1142	an alteration	1130:1142	an alteration of cellular ultra-structures	1130:1171	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	3	63	theme	β-carotene	418:427	arg1	content					430:436	high provitamin A (β-carotene) content	399:436	high provitamin A (β-carotene) content	399:436	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	5	64	theme	root	724:727	arg1	phenotypes					729:738	five different sweet potato storage root phenotypes	688:738	five different sweet potato storage root phenotypes	688:738	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	6	65	theme	starch	888:893	arg1	correlation					873:883	an indirect correlation	861:883	an indirect correlation of starch and carotenoids	861:909	The metabolite data suggested an indirect correlation of starch and carotenoids through the TCA cycle and pentose phosphate pathway.
36049525	3	66	theme	A	415:415	arg1	content					430:436	high provitamin A (β-carotene) content	399:436	high provitamin A (β-carotene) content	399:436	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	7	67	from	change	979:984	arg1	composition					995:1005	lipid composition	989:1005	lipid composition	989:1005	Furthermore, a change in lipid composition was observed to accommodate the storage of carotenoids in the hydrophilic environment of the amyloplast.
36049525	9	68	theme	previous	1276:1283	arg1	analysis					1301:1308	previous gene expression analysis	1276:1308	previous gene expression analysis	1276:1308	This corroborates with previous gene expression analysis through biochemical analysis of sweet potato root tissue.
36049525	2	69	theme	Ipomoea	233:239	arg1	potato					263:268	orange sweet potato	250:268	orange sweet potato (OSP)	250:274	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	2	69	theme	Ipomoea	233:239	arg1	batatas					241:247	Ipomoea batatas	233:247	Ipomoea batatas	233:247	Ipomoea batatas, orange sweet potato (OSP), is one of the biofortification solutions being implemented by the World Health Organisation (WHO) to combat VAD.
36049525	0	70	theme	specialised	71:81	arg1	phenotypes					127:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	specialised Ipomoea batatas (sweet potato) storage root phenotypes	71:136	Changes in carbon allocation and subplastidal amyloplast structures of specialised Ipomoea batatas (sweet potato) storage root phenotypes.
36049525	3	71	theme	adverse	526:532	arg1	effects					534:540	adverse effects	526:540	adverse effects on consumer traits	526:559	However, high provitamin A (β-carotene) content has been associated with a reduction in dry matter, reducing calorific value and having adverse effects on consumer traits.
36049525	5	72	theme	spatial	767:773	arg1	metabolomics					775:786	spatial metabolomics	767:786	spatial metabolomics	767:786	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	1	73	theme	Vitamin	139:145	arg1	VAD					161:163	VAD	161:163	VAD	161:163	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	1	73	theme	Vitamin	139:145	arg1	deficiency					149:158	Vitamin A deficiency	139:158	Vitamin A deficiency (VAD) in Low and Medium Income countries	139:199	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	1	74	theme	major	211:215	arg1	concern					224:230	a major health concern	209:230	a major health concern	209:230	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	8	75	theme	metabolism	1193:1202	arg1	perturbation					1177:1188	perturbation	1177:1188	perturbation of metabolism in high β-carotene producing sweet potato roots	1177:1250	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	8	75	theme	metabolism	1193:1202	arg1	alteration					1133:1142	an alteration	1130:1142	an alteration of cellular ultra-structures	1130:1171	The data suggests an alteration of cellular ultra-structures and perturbation of metabolism in high β-carotene producing sweet potato roots.
36049525	5	76	theme	potato	709:714	arg1	phenotypes					729:738	five different sweet potato storage root phenotypes	688:738	five different sweet potato storage root phenotypes	688:738	Hence, five different sweet potato storage root phenotypes were characterized through spatial metabolomics and proteomics at the sub-plastidal level.
36049525	1	77	theme	A	147:147	arg1	VAD					161:163	VAD	161:163	VAD	161:163	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	1	77	theme	A	147:147	arg1	deficiency					149:158	Vitamin A deficiency	139:158	Vitamin A deficiency (VAD) in Low and Medium Income countries	139:199	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
36049525	1	78	theme	health	217:222	arg1	concern					224:230	a major health concern	209:230	a major health concern	209:230	Vitamin A deficiency (VAD) in Low and Medium Income countries remains a major health concern.
37105871	13	0	theme	N	2222:2222	arg1	efficiency					2224:2233	milk N efficiency	2217:2233	milk N efficiency	2217:2233	Milk and plasma urea N increased, and milk N efficiency decreased in cows fed RSPA and RSPA+AA versus HS and HS+AA.
37105871	7	1	theme	ground	1435:1440	arg1	corn					1442:1445	26% ground corn	1431:1445	26% ground corn	1431:1445	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	17	2	theme	milk	2912:2915	arg1	yield					2917:2921	milk yield	2912:2921	milk yield	2912:2921	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	0	3	theme	nutrient	161:168	arg1	utilization					170:180	nutrient utilization	161:180	nutrient utilization	161:180	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	1	4	theme	starch	244:249	arg1	concentration					251:263	reduced starch concentration	236:263	reduced starch concentration	236:263	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	15	5	theme	supplemental	2540:2551	arg1	RP-MLH					2553:2558	supplemental RP-MLH	2540:2558	supplemental RP-MLH	2540:2558	Plasma Met and His concentrations increased with supplemental RP-MLH.
37105871	7	6	theme	26	1431:1432	arg1	%					1433:1433	%	1433:1433	%	1433:1433	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	4	7	theme	body	944:947	arg1	weight					949:954	body weight	944:954	body weight	944:954	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	7	8	dep	%	1524:1524	arg1	acid					1535:1538	palmitic acid	1526:1538	1.5% palmitic acid	1521:1538	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	5	9	theme	data	1189:1192	arg1	collection					1205:1214	data and sample collection	1189:1214	collection	1205:1214	Each period lasted 21 d, consisting of 14 d for diet adaptation and 7 d for data and sample collection.
37105871	10	10	theme	milk	1850:1853	arg1	yield					1855:1859	milk yield	1850:1859	milk yield	1850:1859	Diets did not affect DM intake and milk yield.
37105871	14	11	theme	total	2407:2411	arg1	excretion					2415:2423	urinary urea N and total N excretion	2388:2423	excretion	2415:2423	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	15	12	theme	His	2506:2508	arg1	concentrations					2510:2523	Plasma Met and His concentrations	2491:2523	concentrations	2510:2523	Plasma Met and His concentrations increased with supplemental RP-MLH.
37105871	17	13	theme	fat	2849:2851	arg1	yield					2853:2857	RP-MLH increased milk fat yield	2827:2857	RP-MLH increased milk fat yield	2827:2857	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	3	14	theme	low	720:722	arg1	diets					746:750	low metabolizable protein diets	720:750	low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	720:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	14	theme	low	720:722	arg1	balance					768:774	mean = -68 g/d balance	753:774	mean = -68 g/d balance	753:774	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	5	15	theme	sample	1198:1203	arg1	collection					1205:1214	data and sample collection	1189:1214	collection	1205:1214	Each period lasted 21 d, consisting of 14 d for diet adaptation and 7 d for data and sample collection.
37105871	3	16	theme	acid-enriched	574:586	arg1	supplement					588:597	a palmitic acid-enriched supplement	563:597	a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	563:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	4	17	theme	Holstein	857:864	arg1	cows					866:869	Sixteen multiparous Holstein cows	837:869	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study	837:1009	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	7	18	contain	had	1482:1484	arg2	%					1524:1524	1.5%	1521:1524	1.5% palmitic acid	1521:1538	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	18	contain	had	1482:1484	arg1	diet					1477:1480	the RSPA diet	1468:1480	the RSPA diet	1468:1480	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	18	contain	had	1482:1484	arg2	%					1505:1505	22%	1503:1505	22% soyhulls	1503:1514	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	18	contain	had	1482:1484	arg2	%					1488:1488	10%	1486:1488	10% ground corn	1486:1500	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	0	19	theme	rumen-protected	106:120	arg1	acids					128:132	rumen-protected amino acids	106:132	rumen-protected amino acids	106:132	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	14	20	theme	urinary	2388:2394	arg1	N					2401:2401	urinary urea N and total N excretion	2388:2423	N	2401:2401	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	4	21	used	used	1016:1019	arg2	cows					866:869	Sixteen multiparous Holstein cows	837:869	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study	837:1009	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	8	22	dep	%	1577:1577	arg1	basis					1566:1570	DM basis	1563:1570	DM basis	1563:1570	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	3	23	theme	matter	606:611	arg1	intake					618:623	dry matter (DM) intake	602:623	dry matter (DM) intake	602:623	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	9	24	dep	%	1734:1734	arg1	silage					1741:1746	corn silage	1736:1746	40% corn silage	1732:1746	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	17	25	theme	maintained	2887:2896	arg1	intake					2901:2906	fiber digestibility and maintained DM intake	2863:2906	intake	2901:2906	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	3	26	theme	protein	738:744	arg1	diets					746:750	low metabolizable protein diets	720:750	low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	720:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	26	theme	protein	738:744	arg1	balance					768:774	mean = -68 g/d balance	753:774	mean = -68 g/d balance	753:774	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	13	27	theme	plasma	2188:2193	arg1	N					2200:2200	plasma urea N	2188:2200	plasma urea N	2188:2200	Milk and plasma urea N increased, and milk N efficiency decreased in cows fed RSPA and RSPA+AA versus HS and HS+AA.
37105871	17	28	theme	milk	2943:2946	arg1	efficiency					2950:2959	decreased milk N efficiency	2933:2959	decreased milk N efficiency	2933:2959	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	4	29	theme	replicated	1026:1035	arg1	design					1056:1061	a replicated 4 × 4 Latin square design	1024:1061	a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments	1024:1110	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	3	30	theme	g/d	764:766	arg1	diets					746:750	low metabolizable protein diets	720:750	low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	720:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	30	theme	g/d	764:766	arg1	balance					768:774	mean = -68 g/d balance	753:774	mean = -68 g/d balance	753:774	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	12	31	theme	Milk	2024:2027	arg1	fat					2029:2031	Milk fat	2024:2031	Milk fat concentration	2024:2045	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	3	32	theme	rumen-protected	793:807	arg1	Met					809:811	rumen-protected Met	793:811	rumen-protected Met	793:811	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	4	33	dep	factorial	1076:1084	arg1	×					1072:1072	×	1072:1072	×	1072:1072	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	16	34	theme	energy	2641:2646	arg1	Intake					2561:2566	Intake	2561:2566	Intake of gross energy and digestible energy	2561:2604	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	16	34	theme	energy	2641:2646	arg1	output					2614:2619	the output	2610:2619	the output of urinary and milk energy	2610:2646	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	16	34	theme	energy	2641:2646	arg1	greater					2657:2663	greater	2657:2663	greater	2657:2663	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	3	35	theme	=	758:758	arg1	diets					746:750	low metabolizable protein diets	720:750	low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	720:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	35	theme	=	758:758	arg1	balance					768:774	mean = -68 g/d balance	753:774	mean = -68 g/d balance	753:774	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	36	theme	DM	614:615	arg1	intake					618:623	dry matter (DM) intake	602:623	dry matter (DM) intake	602:623	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	17	37	theme	elevated	2965:2972	arg1	excretion					2984:2992	elevated urinary N excretion	2965:2992	elevated urinary N excretion	2965:2992	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	4	38	theme	±	913:913	arg1	d					918:918	112 ± 28 d	909:918	(mean ± standard deviation) 112 ± 28 d in milk	881:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	38	theme	±	913:913	arg1	deviation					898:906	mean ± standard deviation	882:906	mean ± standard deviation	882:906	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	7	39	contain	contained	1410:1418	arg2	basis					1424:1428	DM basis	1421:1428	DM basis	1421:1428	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	39	contain	contained	1410:1418	arg2	soyhulls					1454:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	39	contain	contained	1410:1418	arg1	diet					1405:1408	The HS diet	1398:1408	The HS diet	1398:1408	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	9	40	theme	DM	1722:1723	arg1	basis					1725:1729	DM basis	1722:1729	DM basis	1722:1729	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	0	41	theme	metabolizable	62:74	arg1	diets					84:88	low metabolizable protein diets	58:88	low metabolizable protein diets with or without rumen-protected amino acids	58:132	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	1	42	theme	low	325:327	arg1	diets					351:355	low metabolizable protein diets	325:355	low metabolizable protein diets	325:355	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	4	43	from	kg	938:939	arg1	milk					923:926	milk	923:926	milk	923:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	15	44	theme	Plasma	2491:2496	arg1	Met					2498:2500	Plasma Met and His concentrations	2491:2523	Met	2498:2500	Plasma Met and His concentrations increased with supplemental RP-MLH.
37105871	4	45	theme	mean	882:885	arg1	d					918:918	112 ± 28 d	909:918	(mean ± standard deviation) 112 ± 28 d in milk	881:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	45	theme	mean	882:885	arg1	deviation					898:906	mean ± standard deviation	882:906	mean ± standard deviation	882:906	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	16	46	theme	gross	2571:2575	arg1	energy					2577:2582	gross energy	2571:2582	gross energy	2571:2582	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	9	47	contain	had	1717:1719	arg2	%					1734:1734	40%	1732:1734	40% corn silage	1732:1746	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	9	47	contain	had	1717:1719	arg2	%					1750:1750	5%	1749:1750	5% mixed-mostly grass haylage	1749:1777	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	9	47	contain	had	1717:1719	arg2	%					1781:1781	5%	1780:1781	5% grass hay	1780:1791	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	9	47	contain	had	1717:1719	arg1	diets					1711:1715	All 4 diets	1705:1715	All 4 diets	1705:1715	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	9	47	contain	had	1717:1719	arg2	%					1800:1800	50%	1798:1800	50% concentrate	1798:1812	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	14	48	theme	neutral	2352:2358	arg1	fiber					2370:2374	neutral detergent fiber	2352:2374	neutral detergent fiber	2352:2374	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	1	49	theme	protein	343:349	arg1	diets					351:355	low metabolizable protein diets	325:355	low metabolizable protein diets	325:355	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	6	50	theme	palmitic	1320:1327	arg1	RSPA					1355:1358	RSPA	1355:1358	RSPA	1355:1358	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	6	50	theme	palmitic	1320:1327	arg1	supplement					1343:1352	a palmitic acid-enriched supplement	1318:1352	a palmitic acid-enriched supplement (RSPA)	1318:1359	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	6	51	dep	reduced	1298:1304	arg1	3					1295:1295	3	1295:1295	3	1295:1295	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	4	52	theme	standard	889:896	arg1	d					918:918	112 ± 28 d	909:918	(mean ± standard deviation) 112 ± 28 d in milk	881:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	52	theme	standard	889:896	arg1	deviation					898:906	mean ± standard deviation	882:906	mean ± standard deviation	882:906	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	11	53	theme	energy-corrected	1919:1934	arg1	kg/d					1956:1959	47.0 vs. 44.8 kg/d	1942:1959	47.0 vs. 44.8 kg/d	1942:1959	Contrarily, feeding RSPA and RSPA+AA increased yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d) compared with HS and HS+AA.
37105871	11	53	theme	energy-corrected	1919:1934	arg1	milk					1936:1939	energy-corrected milk	1919:1939	energy-corrected milk (47.0 vs. 44.8 kg/d)	1919:1960	Contrarily, feeding RSPA and RSPA+AA increased yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d) compared with HS and HS+AA.
37105871	14	54	theme	protein	2340:2346	arg1	digestibilites					2316:2329	Apparent total-tract digestibilites	2295:2329	Apparent total-tract digestibilites of crude protein and neutral detergent fiber	2295:2374	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	14	54	theme	protein	2340:2346	arg1	N					2401:2401	urinary urea N and total N excretion	2388:2423	N	2401:2401	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	14	54	theme	protein	2340:2346	arg1	excretion					2415:2423	urinary urea N and total N excretion	2388:2423	excretion	2415:2423	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	14	55	theme	Apparent	2295:2302	arg1	digestibilites					2316:2329	Apparent total-tract digestibilites	2295:2329	Apparent total-tract digestibilites of crude protein and neutral detergent fiber	2295:2374	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	6	56	dep	RSPA	1370:1373	arg1	4					1367:1367	4	1367:1367	4	1367:1367	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	8	57	theme	4	1590:1590	arg1	%					1591:1591	%	1591:1591	%	1591:1591	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	2	58	theme	production	445:454	arg1	losses					456:461	production losses	445:461	production losses	445:461	Supplementation of reduced-starch diets with a lipid source may attenuate or eliminate production losses.
37105871	0	59	from	soyhulls	27:34	arg1	diets					84:88	low metabolizable protein diets	58:88	low metabolizable protein diets with or without rumen-protected amino acids	58:132	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	17	60	theme	RP-MLH	2827:2832	arg1	yield					2853:2857	RP-MLH increased milk fat yield	2827:2857	RP-MLH increased milk fat yield	2827:2857	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	4	61	theme	×	1039:1039	arg1	design					1056:1061	a replicated 4 × 4 Latin square design	1024:1061	a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments	1024:1110	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	2	62	theme	reduced-starch	377:390	arg1	diets					392:396	reduced-starch diets	377:396	reduced-starch diets	377:396	Supplementation of reduced-starch diets with a lipid source may attenuate or eliminate production losses.
37105871	14	63	theme	fiber	2370:2374	arg1	digestibilites					2316:2329	Apparent total-tract digestibilites	2295:2329	Apparent total-tract digestibilites of crude protein and neutral detergent fiber	2295:2374	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	14	63	theme	fiber	2370:2374	arg1	N					2401:2401	urinary urea N and total N excretion	2388:2423	N	2401:2401	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	14	63	theme	fiber	2370:2374	arg1	excretion					2415:2423	urinary urea N and total N excretion	2388:2423	excretion	2415:2423	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	8	64	theme	ether	1593:1597	arg1	extract					1599:1605	4% ether extract	1590:1605	4% ether extract	1590:1605	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	12	65	theme	×	2156:2156	arg1	RS					2139:2140	RS	2139:2140	RS (starch level × RP-MLH interaction)	2139:2176	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	12	65	theme	×	2156:2156	arg1	interaction					2165:2175	starch level × RP-MLH interaction	2143:2175	starch level × RP-MLH interaction	2143:2175	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	14	66	theme	total-tract	2304:2314	arg1	digestibilites					2316:2329	Apparent total-tract digestibilites	2295:2329	Apparent total-tract digestibilites of crude protein and neutral detergent fiber	2295:2374	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	1	67	theme	milk	285:288	arg1	yields					275:280	yields	275:280	yields of milk and milk protein	275:305	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	7	68	theme	ground	1490:1495	arg1	corn					1497:1500	ground corn	1490:1500	10% ground corn	1486:1500	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	9	69	dep	had	1717:1719	arg1	basis					1725:1729	DM basis	1722:1729	DM basis	1722:1729	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	9	70	theme	mixed-mostly	1752:1763	arg1	haylage					1771:1777	mixed-mostly grass haylage	1752:1777	5% mixed-mostly grass haylage	1749:1777	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	2	71	theme	lipid	405:409	arg1	source					411:416	a lipid source	403:416	a lipid source	403:416	Supplementation of reduced-starch diets with a lipid source may attenuate or eliminate production losses.
37105871	8	72	theme	32.6	1573:1576	arg1	%					1577:1577	%	1577:1577	%	1577:1577	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	1	73	theme	milk	294:297	arg1	protein					299:305	milk protein	294:305	milk protein	294:305	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	8	74	theme	starch	1614:1619	arg1	concentrations					1639:1652	starch and ether extract concentrations	1614:1652	starch and ether extract concentrations of the RSPA diet	1614:1669	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	3	75	theme	milk	626:629	arg1	yield					631:635	milk yield	626:635	milk yield	626:635	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	0	76	theme	palmitic	41:48	arg1	acid					50:53	palmitic acid	41:53	palmitic acid	41:53	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	4	77	from	d	918:918	arg1	milk					923:926	milk	923:926	milk	923:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	8	78	theme	ether	1625:1629	arg1	extract					1631:1637	ether extract	1625:1637	ether extract	1625:1637	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	17	79	theme	palmitic	2779:2786	arg1	acid					2788:2791	palmitic acid	2779:2791	palmitic acid	2779:2791	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	3	80	theme	AA	661:662	arg1	concentration					664:676	plasma AA concentration	654:676	plasma AA concentration	654:676	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	6	81	theme	high	1248:1251	arg1	HS					1270:1271	(2) HS	1266:1271	(2) HS	1266:1271	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	6	81	theme	high	1248:1251	arg1	RP-MLH					1278:1283	RP-MLH	1278:1283	RP-MLH (HS+AA)	1278:1291	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	6	81	theme	high	1248:1251	arg1	HS					1261:1262	HS	1261:1262	HS	1261:1262	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	6	81	theme	high	1248:1251	arg1	starch					1253:1258	(1) high starch	1244:1258	(1) high starch (HS)	1244:1263	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	2	82	with	Supplementation	358:372	arg1	source					411:416	a lipid source	403:416	a lipid source	403:416	Supplementation of reduced-starch diets with a lipid source may attenuate or eliminate production losses.
37105871	1	83	with	diets	225:229	arg1	concentration					251:263	reduced starch concentration	236:263	reduced starch concentration	236:263	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	9	84	dep	%	1800:1800	arg1	concentrate					1802:1812	concentrate	1802:1812	50% concentrate	1798:1812	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	4	85	theme	±	964:964	arg1	kg/d					968:971	46 ± 5 kg/d	961:971	46 ± 5 kg/d of milk	961:979	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	17	86	theme	DM	2898:2899	arg1	intake					2901:2906	fiber digestibility and maintained DM intake	2863:2906	intake	2901:2906	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	9	87	theme	grass	1783:1787	arg1	hay					1789:1791	grass hay	1783:1791	5% grass hay	1780:1791	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	13	88	theme	milk	2217:2220	arg1	efficiency					2224:2233	milk N efficiency	2217:2233	milk N efficiency	2217:2233	Milk and plasma urea N increased, and milk N efficiency decreased in cows fed RSPA and RSPA+AA versus HS and HS+AA.
37105871	0	89	dep	Replacing	0:8	arg1	Effects					135:141	Effects	135:141	Effects on production and nutrient utilization in dairy cows	135:194	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	6	90	dep	starch	1253:1258	arg1	1					1245:1245	1	1245:1245	1	1245:1245	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	11	91	theme	fat	1971:1973	arg1	yields					1909:1914	yields	1909:1914	yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d)	1909:1994	Contrarily, feeding RSPA and RSPA+AA increased yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d) compared with HS and HS+AA.
37105871	7	92	dep	%	1488:1488	arg1	corn					1497:1500	ground corn	1490:1500	10% ground corn	1486:1500	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	1	93	theme	reduced	236:242	arg1	concentration					251:263	reduced starch concentration	236:263	reduced starch concentration	236:263	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	8	94	theme	diet	1666:1669	arg1	concentrations					1639:1652	starch and ether extract concentrations	1614:1652	starch and ether extract concentrations of the RSPA diet	1614:1669	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	4	95	theme	weight	949:954	arg1	kg					938:939	724 ± 44 kg	929:939	724 ± 44 kg of body weight	929:954	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	95	theme	weight	949:954	arg1	d					918:918	112 ± 28 d	909:918	(mean ± standard deviation) 112 ± 28 d in milk	881:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	95	theme	weight	949:954	arg1	deviation					898:906	mean ± standard deviation	882:906	mean ± standard deviation	882:906	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	95	theme	weight	949:954	arg1	kg/d					968:971	46 ± 5 kg/d	961:971	46 ± 5 kg/d of milk	961:979	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	0	96	from	production	146:155	arg1	cows					191:194	dairy cows	185:194	dairy cows	185:194	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	0	97	theme	dairy	185:189	arg1	cows					191:194	dairy cows	185:194	dairy cows	185:194	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	7	98	theme	DM	1421:1422	arg1	basis					1424:1428	DM basis	1421:1428	DM basis	1421:1428	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	98	theme	DM	1421:1422	arg1	soyhulls					1454:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	9	99	dep	%	1781:1781	arg1	hay					1789:1791	grass hay	1783:1791	5% grass hay	1780:1791	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	0	100	from	Effects	135:141	arg1	production					146:155	production	146:155	production	146:155	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	0	100	from	Effects	135:141	arg1	utilization					170:180	nutrient utilization	161:180	nutrient utilization	161:180	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	3	101	theme	ground	532:537	arg1	corn					539:542	ground corn	532:542	ground corn	532:542	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	14	102	theme	N	2413:2413	arg1	excretion					2415:2423	urinary urea N and total N excretion	2388:2423	excretion	2415:2423	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	17	103	theme	milk	2844:2847	arg1	yield					2853:2857	RP-MLH increased milk fat yield	2827:2857	RP-MLH increased milk fat yield	2827:2857	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	3	104	theme	energy	689:694	arg1	utilization					696:706	energy utilization	689:706	energy utilization	689:706	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	105	theme	palmitic	565:572	arg1	supplement					588:597	a palmitic acid-enriched supplement	563:597	a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	563:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	16	106	theme	energy	2577:2582	arg1	Intake					2561:2566	Intake	2561:2566	Intake of gross energy and digestible energy	2561:2604	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	16	106	theme	energy	2577:2582	arg1	output					2614:2619	the output	2610:2619	the output of urinary and milk energy	2610:2646	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	16	106	theme	energy	2577:2582	arg1	greater					2657:2663	greater	2657:2663	greater	2657:2663	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	6	107	dep	HS	1270:1271	arg1	2					1267:1267	2	1267:1267	2	1267:1267	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	3	108	theme	plasma	654:659	arg1	concentration					664:676	plasma AA concentration	654:676	plasma AA concentration	654:676	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	109	theme	metabolizable	724:736	arg1	diets					746:750	low metabolizable protein diets	720:750	low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	720:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	109	theme	metabolizable	724:736	arg1	balance					768:774	mean = -68 g/d balance	753:774	mean = -68 g/d balance	753:774	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	0	110	theme	amino	122:126	arg1	acids					128:132	rumen-protected amino acids	106:132	rumen-protected amino acids	106:132	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	14	111	theme	urea	2396:2399	arg1	N					2401:2401	urinary urea N and total N excretion	2388:2423	N	2401:2401	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	4	112	with	design	1056:1061	arg1	arrangement					1086:1096	a 2 × 2 factorial arrangement	1068:1096	a 2 × 2 factorial arrangement of treatments	1068:1110	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	113	theme	multiparous	845:855	arg1	cows					866:869	Sixteen multiparous Holstein cows	837:869	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study	837:1009	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	17	114	theme	N	2982:2982	arg1	excretion					2984:2992	elevated urinary N excretion	2965:2992	elevated urinary N excretion	2965:2992	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	4	115	theme	milk	976:979	arg1	kg					938:939	724 ± 44 kg	929:939	724 ± 44 kg of body weight	929:954	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	115	theme	milk	976:979	arg1	d					918:918	112 ± 28 d	909:918	(mean ± standard deviation) 112 ± 28 d in milk	881:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	115	theme	milk	976:979	arg1	deviation					898:906	mean ± standard deviation	882:906	mean ± standard deviation	882:906	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	115	theme	milk	976:979	arg1	kg/d					968:971	46 ± 5 kg/d	961:971	46 ± 5 kg/d of milk	961:979	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	12	116	theme	level	2150:2154	arg1	RS					2139:2140	RS	2139:2140	RS (starch level × RP-MLH interaction)	2139:2176	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	12	116	theme	level	2150:2154	arg1	interaction					2165:2175	starch level × RP-MLH interaction	2143:2175	starch level × RP-MLH interaction	2143:2175	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	17	117	theme	fiber	2863:2867	arg1	digestibility					2869:2881	fiber digestibility and maintained DM intake	2863:2906	digestibility	2869:2881	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	3	118	theme	dry	602:604	arg1	intake					618:623	dry matter (DM) intake	602:623	dry matter (DM) intake	602:623	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	4	119	from	kg/d	968:971	arg1	milk					923:926	milk	923:926	milk	923:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	11	120	theme	milk	1936:1939	arg1	yields					1909:1914	yields	1909:1914	yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d)	1909:1994	Contrarily, feeding RSPA and RSPA+AA increased yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d) compared with HS and HS+AA.
37105871	3	121	theme	mean	753:756	arg1	diets					746:750	low metabolizable protein diets	720:750	low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	720:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	121	theme	mean	753:756	arg1	balance					768:774	mean = -68 g/d balance	753:774	mean = -68 g/d balance	753:774	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	17	122	theme	decreased	2933:2941	arg1	efficiency					2950:2959	decreased milk N efficiency	2933:2959	decreased milk N efficiency	2933:2959	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	7	123	theme	palmitic	1526:1533	arg1	acid					1535:1538	palmitic acid	1526:1538	1.5% palmitic acid	1521:1538	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	16	124	theme	milk	2636:2639	arg1	energy					2641:2646	urinary and milk energy	2624:2646	energy	2641:2646	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	12	125	theme	fat	2029:2031	arg1	concentration					2033:2045	Milk fat concentration	2024:2045	Milk fat concentration	2024:2045	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	17	126	from	soyhulls	2765:2772	arg1	diets					2796:2800	diets	2796:2800	diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion	2796:2992	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	13	127	theme	urea	2195:2198	arg1	N					2200:2200	plasma urea N	2188:2200	plasma urea N	2188:2200	Milk and plasma urea N increased, and milk N efficiency decreased in cows fed RSPA and RSPA+AA versus HS and HS+AA.
37105871	17	128	theme	N	2948:2948	arg1	efficiency					2950:2959	decreased milk N efficiency	2933:2959	decreased milk N efficiency	2933:2959	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	4	129	dep	arrangement	1086:1096	arg1	factorial					1076:1084	factorial	1076:1084	factorial	1076:1084	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	130	theme	study	1005:1009	arg1	beginning					988:996	the beginning	984:996	the beginning of the study	984:1009	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	0	131	theme	low	58:60	arg1	diets					84:88	low metabolizable protein diets	58:88	low metabolizable protein diets with or without rumen-protected amino acids	58:132	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	12	132	theme	starch	2143:2148	arg1	RS					2139:2140	RS	2139:2140	RS (starch level × RP-MLH interaction)	2139:2176	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	12	132	theme	starch	2143:2148	arg1	interaction					2165:2175	starch level × RP-MLH interaction	2143:2175	starch level × RP-MLH interaction	2143:2175	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	9	133	dep	%	1750:1750	arg1	haylage					1771:1777	mixed-mostly grass haylage	1752:1777	5% mixed-mostly grass haylage	1749:1777	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	12	134	theme	RP-MLH	2158:2163	arg1	RS					2139:2140	RS	2139:2140	RS (starch level × RP-MLH interaction)	2139:2176	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	12	134	theme	RP-MLH	2158:2163	arg1	interaction					2165:2175	starch level × RP-MLH interaction	2143:2175	starch level × RP-MLH interaction	2143:2175	Milk fat concentration tended to decrease when RP-MLH was supplemented to HS, but no change was seen when added to RS (starch level × RP-MLH interaction).
37105871	0	135	theme	protein	76:82	arg1	diets					84:88	low metabolizable protein diets	58:88	low metabolizable protein diets with or without rumen-protected amino acids	58:132	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	9	136	theme	corn	1736:1739	arg1	silage					1741:1746	corn silage	1736:1746	40% corn silage	1732:1746	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	17	137	theme	urinary	2974:2980	arg1	excretion					2984:2992	elevated urinary N excretion	2965:2992	elevated urinary N excretion	2965:2992	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	14	138	theme	detergent	2360:2368	arg1	fiber					2370:2374	neutral detergent fiber	2352:2374	neutral detergent fiber	2352:2374	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	11	139	theme	feeding	1874:1880	arg1	RSPA					1882:1885	feeding RSPA	1874:1885	feeding RSPA	1874:1885	Contrarily, feeding RSPA and RSPA+AA increased yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d) compared with HS and HS+AA.
37105871	8	140	theme	HS	1545:1546	arg1	diet					1548:1551	The HS diet	1541:1551	The HS diet	1541:1551	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	1	141	theme	metabolizable	329:341	arg1	diets					351:355	low metabolizable protein diets	325:355	low metabolizable protein diets	325:355	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	6	142	theme	acid-enriched	1329:1341	arg1	RSPA					1355:1358	RSPA	1355:1358	RSPA	1355:1358	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	6	142	theme	acid-enriched	1329:1341	arg1	supplement					1343:1352	a palmitic acid-enriched supplement	1318:1352	a palmitic acid-enriched supplement (RSPA)	1318:1359	Diets were fed as follows: (1) high starch (HS), (2) HS plus RP-MLH (HS+AA), (3) reduced starch plus a palmitic acid-enriched supplement (RSPA), and (4) RSPA plus RP-MLH (RSPA+AA).
37105871	7	143	theme	corn	1442:1445	arg1	basis					1424:1428	DM basis	1421:1428	DM basis	1421:1428	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	143	theme	corn	1442:1445	arg1	soyhulls					1454:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	16	144	theme	digestible	2588:2597	arg1	energy					2599:2604	digestible energy	2588:2604	digestible energy	2588:2604	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	4	145	theme	2	1070:1070	arg1	×					1072:1072	×	1072:1072	×	1072:1072	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	8	146	theme	%	1591:1591	arg1	extract					1599:1605	4% ether extract	1590:1605	4% ether extract	1590:1605	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	4	147	theme	±	887:887	arg1	d					918:918	112 ± 28 d	909:918	(mean ± standard deviation) 112 ± 28 d in milk	881:926	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	4	147	theme	±	887:887	arg1	deviation					898:906	mean ± standard deviation	882:906	mean ± standard deviation	882:906	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	2	148	theme	diets	392:396	arg1	Supplementation					358:372	Supplementation	358:372	Supplementation of reduced-starch diets with a lipid source	358:416	Supplementation of reduced-starch diets with a lipid source may attenuate or eliminate production losses.
37105871	0	149	theme	ground	10:15	arg1	corn					17:20	ground corn	10:20	ground corn	10:20	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	17	150	theme	increased	2834:2842	arg1	yield					2853:2857	RP-MLH increased milk fat yield	2827:2857	RP-MLH increased milk fat yield	2827:2857	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	4	151	theme	square	1049:1054	arg1	design					1056:1061	a replicated 4 × 4 Latin square design	1024:1061	a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments	1024:1110	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	3	152	from	supplement	588:597	arg1	utilization					696:706	energy utilization	689:706	energy utilization	689:706	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	152	from	supplement	588:597	arg1	yield					631:635	milk yield	626:635	milk yield	626:635	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	152	from	supplement	588:597	arg1	concentration					664:676	plasma AA concentration	654:676	plasma AA concentration	654:676	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	152	from	supplement	588:597	arg1	cows					711:714	cows	711:714	cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH)	711:834	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	152	from	supplement	588:597	arg1	N					683:683	N	683:683	N	683:683	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	152	from	supplement	588:597	arg1	composition					641:651	composition	641:651	composition	641:651	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	3	152	from	supplement	588:597	arg1	intake					618:623	dry matter (DM) intake	602:623	dry matter (DM) intake	602:623	Our objective was to investigate the effects of partially replacing ground corn with soyhulls plus a palmitic acid-enriched supplement on dry matter (DM) intake, milk yield and composition, plasma AA concentration, and N and energy utilization in cows fed low metabolizable protein diets (mean = -68 g/d balance) with or without rumen-protected Met, Lys, and His (RP-MLH).
37105871	4	153	theme	±	933:933	arg1	kg					938:939	724 ± 44 kg	929:939	724 ± 44 kg of body weight	929:954	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	0	154	from	utilization	170:180	arg1	cows					191:194	dairy cows	185:194	dairy cows	185:194	Replacing ground corn with soyhulls plus palmitic acid in low metabolizable protein diets with or without rumen-protected amino acids: Effects on production and nutrient utilization in dairy cows.
37105871	17	155	theme	ground	2748:2753	arg1	corn					2755:2758	ground corn	2748:2758	ground corn	2748:2758	In summary, partially replacing ground corn with soyhulls plus palmitic acid in diets supplemented or not with RP-MLH increased milk fat yield and fiber digestibility and maintained DM intake and milk yield, but with decreased milk N efficiency and elevated urinary N excretion.
37105871	4	156	theme	treatments	1101:1110	arg1	arrangement					1086:1096	a 2 × 2 factorial arrangement	1068:1096	a 2 × 2 factorial arrangement of treatments	1068:1110	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	8	157	theme	%	1577:1577	arg1	starch					1579:1584	(DM basis) 32.6% starch	1562:1584	(DM basis) 32.6% starch	1562:1584	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	16	158	theme	feeding	2670:2676	arg1	RSPA					2678:2681	feeding RSPA	2670:2681	feeding RSPA	2670:2681	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	9	159	theme	grass	1765:1769	arg1	haylage					1771:1777	mixed-mostly grass haylage	1752:1777	5% mixed-mostly grass haylage	1749:1777	All 4 diets had (DM basis) 40% corn silage, 5% mixed-mostly grass haylage, 5% grass hay, and 50% concentrate.
37105871	1	160	theme	protein	299:305	arg1	yields					275:280	yields	275:280	yields of milk and milk protein	275:305	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	7	161	theme	RSPA	1472:1475	arg1	diet					1477:1480	the RSPA diet	1468:1480	the RSPA diet	1468:1480	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	8	162	theme	extract	1631:1637	arg1	concentrations					1639:1652	starch and ether extract concentrations	1614:1652	starch and ether extract concentrations of the RSPA diet	1614:1669	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	7	163	dep	%	1505:1505	arg1	soyhulls					1507:1514	soyhulls	1507:1514	22% soyhulls	1503:1514	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	4	164	theme	2	1074:1074	arg1	×					1072:1072	×	1072:1072	×	1072:1072	Sixteen multiparous Holstein cows averaging (mean ± standard deviation) 112 ± 28 d in milk, 724 ± 44 kg of body weight, and 46 ± 5 kg/d of milk in the beginning of the study were used in a replicated 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments.
37105871	16	165	theme	urinary	2624:2630	arg1	energy					2641:2646	urinary and milk energy	2624:2646	energy	2641:2646	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	1	166	theme	dairy	310:314	arg1	cows					316:319	dairy cows	310:319	dairy cows fed low metabolizable protein diets	310:355	We previously observed that diets with reduced starch concentration decreased yields of milk and milk protein in dairy cows fed low metabolizable protein diets.
37105871	16	167	theme	energy	2599:2604	arg1	Intake					2561:2566	Intake	2561:2566	Intake of gross energy and digestible energy	2561:2604	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	16	167	theme	energy	2599:2604	arg1	output					2614:2619	the output	2610:2619	the output of urinary and milk energy	2610:2646	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	16	167	theme	energy	2599:2604	arg1	greater					2657:2663	greater	2657:2663	greater	2657:2663	Intake of gross energy and digestible energy and the output of urinary and milk energy were all greater with feeding RSPA and RSPA+AA versus HS and HS+AA.
37105871	7	168	theme	%	1452:1452	arg1	basis					1424:1428	DM basis	1421:1428	DM basis	1421:1428	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	168	theme	%	1452:1452	arg1	soyhulls					1454:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	(DM basis) 26% ground corn and 7% soyhulls	1420:1461	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	7	169	theme	%	1433:1433	arg1	corn					1442:1445	26% ground corn	1431:1445	26% ground corn	1431:1445	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	14	170	theme	crude	2334:2338	arg1	protein					2340:2346	crude protein	2334:2346	crude protein	2334:2346	Apparent total-tract digestibilites of crude protein and neutral detergent fiber, as well as urinary urea N and total N excretion, were greater in cows offered RSPA and RSPA+AA than HS and HS+AA.
37105871	10	171	theme	DM	1836:1837	arg1	intake					1839:1844	DM intake	1836:1844	DM intake	1836:1844	Diets did not affect DM intake and milk yield.
37105871	8	172	theme	RSPA	1661:1664	arg1	diet					1666:1669	the RSPA diet	1657:1669	the RSPA diet	1657:1669	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
37105871	7	173	theme	HS	1402:1403	arg1	diet					1405:1408	The HS diet	1398:1408	The HS diet	1398:1408	The HS diet contained (DM basis) 26% ground corn and 7% soyhulls, and the RSPA diet had 10% ground corn, 22% soyhulls, and 1.5% palmitic acid.
37105871	11	174	theme	milk	1966:1969	arg1	kg/d					1990:1993	1.65 vs. 1.50 kg/d	1976:1993	1.65 vs. 1.50 kg/d	1976:1993	Contrarily, feeding RSPA and RSPA+AA increased yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d) compared with HS and HS+AA.
37105871	11	174	theme	milk	1966:1969	arg1	fat					1971:1973	milk fat	1966:1973	milk fat (1.65 vs. 1.50 kg/d)	1966:1994	Contrarily, feeding RSPA and RSPA+AA increased yields of energy-corrected milk (47.0 vs. 44.8 kg/d) and milk fat (1.65 vs. 1.50 kg/d) compared with HS and HS+AA.
37105871	5	175	theme	diet	1161:1164	arg1	adaptation					1166:1175	diet adaptation	1161:1175	diet adaptation	1161:1175	Each period lasted 21 d, consisting of 14 d for diet adaptation and 7 d for data and sample collection.
37105871	8	176	theme	DM	1563:1564	arg1	basis					1566:1570	DM basis	1563:1570	DM basis	1563:1570	The HS diet averaged (DM basis) 32.6% starch and 4% ether extract, while starch and ether extract concentrations of the RSPA diet were 21.7 and 5.9%, respectively.
36521060	9	0	theme	IPEC-J2	1648:1654	arg1	cells					1641:1645	porcine intestinal epithelial cells	1611:1645	porcine intestinal epithelial cells (IPEC-J2 cells)	1611:1661	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	0	theme	IPEC-J2	1648:1654	arg1	cells					1656:1660	IPEC-J2 cells	1648:1660	IPEC-J2 cells	1648:1660	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	7	1	theme	LPS-challenge	1258:1270	arg1	mice					1272:1275	LPS-challenge mice	1258:1275	LPS-challenge mice	1258:1275	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	4	2	theme	proinflammatory	663:677	arg1	cytokines					679:687	proinflammatory cytokines	663:687	proinflammatory cytokines production	663:698	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	10	3	theme	integrity	2017:2025	arg1	maintenance					1982:1992	maintenance	1982:1992	maintenance of gut mucosal barrier integrity	1982:2025	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	10	3	theme	integrity	2017:2025	arg1	activation					1948:1957	the activation	1944:1957	the activation of metabolic pathways	1944:1979	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	10	3	theme	integrity	2017:2025	arg1	level					2077:2081	AMPK-SIRT1-autophagy level	2056:2081	AMPK-SIRT1-autophagy level	2056:2081	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	10	3	theme	integrity	2017:2025	arg1	composition					2039:2049	microbiota composition	2028:2049	microbiota composition	2028:2049	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	2	4	theme	potential	325:333	arg1	roles					346:350	the potential modulating roles	321:350	the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms	321:457	The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms.
36521060	9	5	theme	similar	1674:1680	arg1	effects					1693:1699	similar protective effects	1674:1699	similar protective effects	1674:1699	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	4	6	theme	pathway	730:736	arg1	activation					738:747	nuclear factor κB (NF-κB) pathway activation	704:747	nuclear factor κB (NF-κB) pathway activation	704:747	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	10	7	theme	mucosal	2001:2007	arg1	integrity					2017:2025	gut mucosal barrier integrity	1997:2025	gut mucosal barrier integrity	1997:2025	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	1	8	theme	intestinal	209:218	arg1	defense					227:233	intestinal immune defense	209:233	intestinal immune defense	209:233	Tryptophan has drawn wide attention due to its involvement in improving intestinal immune defense directly and indirectly by regulating metabolic pathways.
36521060	6	9	theme	autophagy	1165:1173	arg1	homeostasis					1175:1185	autophagy homeostasis	1165:1185	autophagy homeostasis	1165:1185	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	2	10	theme	tryptophan	355:364	arg1	roles					346:350	the potential modulating roles	321:350	the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms	321:457	The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms.
36521060	5	11	theme	antimicrobial	928:940	arg1	AMPs					952:955	AMPs	952:955	AMPs	952:955	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	5	11	theme	antimicrobial	928:940	arg1	peptides					942:949	antimicrobial peptides	928:949	antimicrobial peptides (AMPs)	928:956	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	4	12	theme	κB	719:720	arg1	activation					738:747	nuclear factor κB (NF-κB) pathway activation	704:747	nuclear factor κB (NF-κB) pathway activation	704:747	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	10	13	theme	microbiota	2028:2037	arg1	composition					2039:2049	microbiota composition	2028:2049	microbiota composition	2028:2049	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	5	14	theme	goblet	902:907	arg1	cells					909:913	goblet cells	902:913	goblet cells	902:913	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	2	15	theme	molecular	438:446	arg1	mechanisms					448:457	the underlying molecular mechanisms	423:457	the underlying molecular mechanisms	423:457	The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms.
36521060	4	16	theme	NF-κB	723:727	arg1	activation					738:747	nuclear factor κB (NF-κB) pathway activation	704:747	nuclear factor κB (NF-κB) pathway activation	704:747	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	9	17	theme	intestinal	1740:1749	arg1	inflammation					1751:1762	LPS-induced intestinal inflammation	1728:1762	LPS-induced intestinal inflammation	1728:1762	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	10	18	theme	AMPK-SIRT1-autophagy	2056:2075	arg1	level					2077:2081	AMPK-SIRT1-autophagy level	2056:2081	AMPK-SIRT1-autophagy level	2056:2081	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	1	19	theme	wide	158:161	arg1	attention					163:171	wide attention	158:171	wide attention	158:171	Tryptophan has drawn wide attention due to its involvement in improving intestinal immune defense directly and indirectly by regulating metabolic pathways.
36521060	10	20	theme	protective	1889:1898	arg1	effects					1921:1927	intestinal protective and immunoregulatory effects	1878:1927	intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level	1878:2081	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	8	21	theme	metabolic	1470:1478	arg1	pathways					1480:1487	metabolic pathways	1470:1487	metabolic pathways	1470:1487	Notably, dietary tryptophan resulted in the activation of metabolic pathways during the inflammatory response.
36521060	6	22	theme	LPS-induced	1025:1035	arg1	flux					1048:1051	LPS-induced autophagic flux	1025:1051	LPS-induced autophagic flux	1025:1051	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	5	23	theme	LPS-caused	829:838	arg1	damage					867:872	LPS-caused intestinal mucosal barrier damage	829:872	LPS-caused intestinal mucosal barrier damage	829:872	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	8	24	theme	pathways	1480:1487	arg1	activation					1456:1465	the activation	1452:1465	the activation of metabolic pathways during the inflammatory response	1452:1520	Notably, dietary tryptophan resulted in the activation of metabolic pathways during the inflammatory response.
36521060	9	25	theme	exogenous	1536:1544	arg1	treatment					1546:1554	exogenous treatment	1536:1554	exogenous treatment of tryptophan metabolites kynurenine (Kyn)	1536:1597	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	26	from	treatment	1546:1554	arg1	cells					1641:1645	porcine intestinal epithelial cells	1611:1645	porcine intestinal epithelial cells (IPEC-J2 cells)	1611:1661	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	26	from	treatment	1546:1554	arg1	cells					1656:1660	IPEC-J2 cells	1648:1660	IPEC-J2 cells	1648:1660	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	8	27	theme	inflammatory	1500:1511	arg1	response					1513:1520	the inflammatory response	1496:1520	the inflammatory response	1496:1520	Notably, dietary tryptophan resulted in the activation of metabolic pathways during the inflammatory response.
36521060	9	28	theme	metabolites	1570:1580	arg1	treatment					1546:1554	exogenous treatment	1536:1554	exogenous treatment of tryptophan metabolites kynurenine (Kyn)	1536:1597	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	28	theme	metabolites	1570:1580	arg1	5-HT					1603:1606	5-HT	1603:1606	5-HT in porcine intestinal epithelial cells (IPEC-J2 cells)	1603:1661	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	0	29	theme	Gut-Derived	0:10	arg1	Metabolites					12:22	Gut-Derived Metabolites	0:22	Gut-Derived Metabolites from Dietary Tryptophan Supplementation	0:62	Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway.
36521060	6	30	theme	protein	1079:1085	arg1	AMPK					1095:1098	AMPK	1095:1098	AMPK	1095:1098	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	6	30	theme	protein	1079:1085	arg1	kinase					1087:1092	the AMP-activated protein kinase	1061:1092	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis	1061:1185	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	7	31	theme	fatty	1320:1324	arg1	SCFA					1332:1335	SCFA	1332:1335	SCFA	1332:1335	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	7	31	theme	fatty	1320:1324	arg1	acid					1326:1329	short-chain fatty acid	1308:1329	short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%)	1308:1409	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	0	32	theme	Tryptophan	37:46	arg1	Supplementation					48:62	Dietary Tryptophan Supplementation	29:62	Dietary Tryptophan Supplementation	29:62	Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway.
36521060	5	33	theme	mucosal	851:857	arg1	damage					867:872	LPS-caused intestinal mucosal barrier damage	829:872	LPS-caused intestinal mucosal barrier damage	829:872	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	7	34	theme	acid	1326:1329	arg1	abundance					1295:1303	the abundance	1291:1303	the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%)	1291:1409	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	3	35	theme	protective	464:473	arg1	effects					475:481	The protective effects	460:481	The protective effects of tryptophan against intestinal inflammation	460:527	The protective effects of tryptophan against intestinal inflammation are examined in the lipopolysaccharide (LPS)-induced inflammatory model.
36521060	5	36	theme	peptides	942:949	arg1	number					892:897	the number	888:897	the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice	888:977	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	10	37	theme	immunoregulatory	1904:1919	arg1	effects					1921:1927	intestinal protective and immunoregulatory effects	1878:1927	intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level	1878:2081	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	6	38	from	pathway	1119:1125	arg1	systems					1145:1151	the intestinal systems	1130:1151	the intestinal systems	1130:1151	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	9	39	theme	porcine	1611:1617	arg1	cells					1641:1645	porcine intestinal epithelial cells	1611:1645	porcine intestinal epithelial cells (IPEC-J2 cells)	1611:1661	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	39	theme	porcine	1611:1617	arg1	cells					1656:1660	IPEC-J2 cells	1648:1660	IPEC-J2 cells	1648:1660	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	7	40	theme	short-chain	1308:1318	arg1	SCFA					1332:1335	SCFA	1332:1335	SCFA	1332:1335	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	7	40	theme	short-chain	1308:1318	arg1	acid					1326:1329	short-chain fatty acid	1308:1329	short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%)	1308:1409	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	9	41	theme	epithelial	1630:1639	arg1	cells					1641:1645	porcine intestinal epithelial cells	1611:1645	porcine intestinal epithelial cells (IPEC-J2 cells)	1611:1661	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	41	theme	epithelial	1630:1639	arg1	cells					1656:1660	IPEC-J2 cells	1648:1660	IPEC-J2 cells	1648:1660	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	4	42	theme	cytokines	679:687	arg1	production					689:698	proinflammatory cytokines production	663:698	proinflammatory cytokines production	663:698	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	8	43	theme	dietary	1421:1427	arg1	tryptophan					1429:1438	dietary tryptophan	1421:1438	dietary tryptophan	1421:1438	Notably, dietary tryptophan resulted in the activation of metabolic pathways during the inflammatory response.
36521060	2	44	theme	underlying	427:436	arg1	mechanisms					448:457	the underlying molecular mechanisms	423:457	the underlying molecular mechanisms	423:457	The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms.
36521060	0	45	theme	Intestinal	71:80	arg1	Inflammation					82:93	Intestinal Inflammation	71:93	Intestinal Inflammation	71:93	Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway.
36521060	6	46	theme	SIRT1	1112:1116	arg1	pathway					1119:1125	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway	1061:1125	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis	1061:1185	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	7	47	theme	gut	1228:1230	arg1	composition					1243:1253	the gut microbiota composition	1224:1253	the gut microbiota composition in LPS-challenge mice	1224:1275	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	10	48	theme	gut	1997:1999	arg1	integrity					2017:2025	gut mucosal barrier integrity	1997:2025	gut mucosal barrier integrity	1997:2025	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	3	49	theme	inflammatory	582:593	arg1	model					595:599	the lipopolysaccharide (LPS)-induced inflammatory model	545:599	the lipopolysaccharide (LPS)-induced inflammatory model	545:599	The protective effects of tryptophan against intestinal inflammation are examined in the lipopolysaccharide (LPS)-induced inflammatory model.
36521060	10	50	theme	pathways	1972:1979	arg1	maintenance					1982:1992	maintenance	1982:1992	maintenance of gut mucosal barrier integrity	1982:2025	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	10	50	theme	pathways	1972:1979	arg1	activation					1948:1957	the activation	1944:1957	the activation of metabolic pathways	1944:1979	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	10	50	theme	pathways	1972:1979	arg1	level					2077:2081	AMPK-SIRT1-autophagy level	2056:2081	AMPK-SIRT1-autophagy level	2056:2081	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	10	50	theme	pathways	1972:1979	arg1	composition					2039:2049	microbiota composition	2028:2049	microbiota composition	2028:2049	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	2	51	theme	modulating	335:344	arg1	roles					346:350	the potential modulating roles	321:350	the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms	321:457	The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms.
36521060	10	52	theme	barrier	2009:2015	arg1	integrity					2017:2025	gut mucosal barrier integrity	1997:2025	gut mucosal barrier integrity	1997:2025	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	5	53	theme	mucins	916:921	arg1	number					892:897	the number	888:897	the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice	888:977	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	9	54	theme	protective	1682:1691	arg1	effects					1693:1699	similar protective effects	1674:1699	similar protective effects	1674:1699	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	7	55	theme	microbiota	1232:1241	arg1	composition					1243:1253	the gut microbiota composition	1224:1253	the gut microbiota composition in LPS-challenge mice	1224:1275	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	10	56	theme	present	1829:1835	arg1	study					1837:1841	the present study	1825:1841	the present study	1825:1841	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	1	57	theme	immune	220:225	arg1	defense					227:233	intestinal immune defense	209:233	intestinal immune defense	209:233	Tryptophan has drawn wide attention due to its involvement in improving intestinal immune defense directly and indirectly by regulating metabolic pathways.
36521060	6	58	theme	p	1005:1005	arg1	tryptophan					993:1002	tryptophan	993:1002	tryptophan (p < 0.05)	993:1013	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	6	58	theme	p	1005:1005	arg1	<					1007:1007	p < 0.05	1005:1012	p < 0.05	1005:1012	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	4	59	theme	factor	712:717	arg1	activation					738:747	nuclear factor κB (NF-κB) pathway activation	704:747	nuclear factor κB (NF-κB) pathway activation	704:747	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	2	60	theme	intestinal	385:394	arg1	inflammation					396:407	intestinal inflammation	385:407	intestinal inflammation	385:407	The study aims to elucidate the potential modulating roles of tryptophan to protect against intestinal inflammation and elucidate the underlying molecular mechanisms.
36521060	5	61	theme	cells	909:913	arg1	number					892:897	the number	888:897	the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice	888:977	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	9	62	theme	LPS-induced	1728:1738	arg1	inflammation					1751:1762	LPS-induced intestinal inflammation	1728:1762	LPS-induced intestinal inflammation	1728:1762	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	63	dep	metabolites	1570:1580	arg1	Kyn					1594:1596	Kyn	1594:1596	Kyn	1594:1596	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	63	dep	metabolites	1570:1580	arg1	kynurenine					1582:1591	kynurenine	1582:1591	tryptophan metabolites kynurenine (Kyn)	1559:1597	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	7	64	dep	±	1384:1384	arg1	to					1392:1393	to	1392:1393	to	1392:1393	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	7	65	from	composition	1243:1253	arg1	mice					1272:1275	LPS-challenge mice	1258:1275	LPS-challenge mice	1258:1275	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	10	66	theme	intestinal	1878:1887	arg1	effects					1921:1927	intestinal protective and immunoregulatory effects	1878:1927	intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level	1878:2081	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	7	67	dep	±	1400:1400	arg1	%					1408:1408	0.0041%	1402:1408	0.0041%	1402:1408	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	6	68	theme	autophagic	1037:1046	arg1	flux					1048:1051	LPS-induced autophagic flux	1025:1051	LPS-induced autophagic flux	1025:1051	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	4	69	theme	LPS	754:756	arg1	challenge					758:766	LPS challenge	754:766	LPS challenge	754:766	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	0	70	theme	AMPK-SIRT1-Autophagy	107:126	arg1	Pathway					128:134	the AMPK-SIRT1-Autophagy Pathway	103:134	the AMPK-SIRT1-Autophagy Pathway	103:134	Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway.
36521060	5	71	theme	p	808:808	arg1	tryptophan					796:805	tryptophan	796:805	tryptophan (p < 0.05)	796:816	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	5	71	theme	p	808:808	arg1	<					810:810	p < 0.05	808:815	p < 0.05	808:815	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	5	72	from	number	892:897	arg1	ileum					965:969	the ileum	961:969	the ileum of mice	961:977	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	7	73	dep	Acetivibrio	1365:1375	arg1	±					1384:1384	±	1384:1384	±	1384:1384	Meanwhile, tryptophan also reshaped the gut microbiota composition in LPS-challenge mice by increasing the abundance of short-chain fatty acid (SCFA)-producing bacteria such as Acetivibrio (0.053 ± 0.017 to 0.21 ± 0.0041%).
36521060	9	74	theme	tryptophan	1559:1568	arg1	metabolites					1570:1580	tryptophan metabolites kynurenine (Kyn)	1559:1597	tryptophan metabolites kynurenine (Kyn)	1559:1597	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	6	75	theme	-sirtuin	1100:1107	arg1	pathway					1119:1125	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway	1061:1125	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis	1061:1185	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	0	76	from	Supplementation	48:62	arg1	Metabolites					12:22	Gut-Derived Metabolites	0:22	Gut-Derived Metabolites from Dietary Tryptophan Supplementation	0:62	Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway.
36521060	4	77	dep	markedly	633:640	arg1	p					643:643	p < 0.01	643:650	p < 0.01	643:650	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	5	78	theme	mice	974:977	arg1	ileum					965:969	the ileum	961:969	the ileum of mice	961:977	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	3	79	theme	intestinal	505:514	arg1	inflammation					516:527	intestinal inflammation	505:527	intestinal inflammation	505:527	The protective effects of tryptophan against intestinal inflammation are examined in the lipopolysaccharide (LPS)-induced inflammatory model.
36521060	0	80	theme	Dietary	29:35	arg1	Supplementation					48:62	Dietary Tryptophan Supplementation	29:62	Dietary Tryptophan Supplementation	29:62	Gut-Derived Metabolites from Dietary Tryptophan Supplementation Quench Intestinal Inflammation through the AMPK-SIRT1-Autophagy Pathway.
36521060	6	81	theme	kinase	1087:1092	arg1	pathway					1119:1125	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway	1061:1125	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis	1061:1185	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	4	82	theme	<	645:645	arg1	p					643:643	p < 0.01	643:650	p < 0.01	643:650	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36521060	5	83	theme	intestinal	840:849	arg1	damage					867:872	LPS-caused intestinal mucosal barrier damage	829:872	LPS-caused intestinal mucosal barrier damage	829:872	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	9	84	from	5-HT	1603:1606	arg1	cells					1641:1645	porcine intestinal epithelial cells	1611:1645	porcine intestinal epithelial cells (IPEC-J2 cells)	1611:1661	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	84	from	5-HT	1603:1606	arg1	cells					1656:1660	IPEC-J2 cells	1648:1660	IPEC-J2 cells	1648:1660	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	6	85	theme	AMP-activated	1065:1077	arg1	AMPK					1095:1098	AMPK	1095:1098	AMPK	1095:1098	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	6	85	theme	AMP-activated	1065:1077	arg1	kinase					1087:1092	the AMP-activated protein kinase	1061:1092	the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis	1061:1185	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	3	86	theme	tryptophan	486:495	arg1	effects					475:481	The protective effects	460:481	The protective effects of tryptophan against intestinal inflammation	460:527	The protective effects of tryptophan against intestinal inflammation are examined in the lipopolysaccharide (LPS)-induced inflammatory model.
36521060	5	87	theme	barrier	859:865	arg1	damage					867:872	LPS-caused intestinal mucosal barrier damage	829:872	LPS-caused intestinal mucosal barrier damage	829:872	Next, we demonstrated that tryptophan (p < 0.05) attenuated LPS-caused intestinal mucosal barrier damage by increasing the number of goblet cells, mucins, and antimicrobial peptides (AMPs) in the ileum of mice.
36521060	6	88	theme	intestinal	1134:1143	arg1	systems					1145:1151	the intestinal systems	1130:1151	the intestinal systems	1130:1151	In addition, tryptophan (p < 0.05) inhibited LPS-induced autophagic flux through the AMP-activated protein kinase (AMPK)-sirtuin 1 (SIRT1) pathway in the intestinal systems to maintain autophagy homeostasis.
36521060	10	89	theme	metabolic	1962:1970	arg1	pathways					1972:1979	metabolic pathways	1962:1979	metabolic pathways	1962:1979	Taken together, the present study indicates that tryptophan exhibits intestinal protective and immunoregulatory effects resulting from the activation of metabolic pathways, maintenance of gut mucosal barrier integrity, microbiota composition, and AMPK-SIRT1-autophagy level.
36521060	3	90	theme	-induced	573:580	arg1	model					595:599	the lipopolysaccharide (LPS)-induced inflammatory model	545:599	the lipopolysaccharide (LPS)-induced inflammatory model	545:599	The protective effects of tryptophan against intestinal inflammation are examined in the lipopolysaccharide (LPS)-induced inflammatory model.
36521060	9	91	theme	intestinal	1619:1628	arg1	cells					1641:1645	porcine intestinal epithelial cells	1611:1645	porcine intestinal epithelial cells (IPEC-J2 cells)	1611:1661	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	9	91	theme	intestinal	1619:1628	arg1	cells					1656:1660	IPEC-J2 cells	1648:1660	IPEC-J2 cells	1648:1660	Furthermore, exogenous treatment of tryptophan metabolites kynurenine (Kyn) and 5-HT in porcine intestinal epithelial cells (IPEC-J2 cells) reproduced similar protective effects as tryptophan to attenuate LPS-induced intestinal inflammation through regulating the AMPK-SIRT1-autophagy.
36521060	1	92	theme	metabolic	273:281	arg1	pathways					283:290	metabolic pathways	273:290	metabolic pathways	273:290	Tryptophan has drawn wide attention due to its involvement in improving intestinal immune defense directly and indirectly by regulating metabolic pathways.
36521060	4	93	theme	nuclear	704:710	arg1	activation					738:747	nuclear factor κB (NF-κB) pathway activation	704:747	nuclear factor κB (NF-κB) pathway activation	704:747	We first found that tryptophan markedly (p < 0.01) inhibited proinflammatory cytokines production and nuclear factor κB (NF-κB) pathway activation upon LPS challenge.
36178853	1	0	theme	proinflammatory	320:334	arg1	responses					336:344	proinflammatory responses	320:344	proinflammatory responses	320:344	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	4	1	theme	inducible	699:707	arg1	iNOS					732:735	iNOS	732:735	iNOS	732:735	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	4	1	theme	inducible	699:707	arg1	synthase					722:729	inducible nitric oxide synthase	699:729	inducible nitric oxide synthase (iNOS)	699:736	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	1	2	theme	2,2-bis[p-	233:242	arg1	propane					288:294	2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane	233:294	2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA)	233:303	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	1	2	theme	2,2-bis[p-	233:242	arg1	BisGMA					297:302	BisGMA	297:302	BisGMA	297:302	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	7	3	theme	p38	1173:1175	arg1	kinase					1214:1219	extracellular signal-regulated kinase	1183:1219	extracellular signal-regulated kinase (ERK)	1183:1225	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	3	theme	p38	1173:1175	arg1	MAPK					1177:1180	p38 MAPK	1173:1180	p38 MAPK	1173:1180	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	3	theme	p38	1173:1175	arg1	kinase					1245:1250	c-Jun N-terminal kinase	1228:1250	c-Jun N-terminal kinase (JNK)	1228:1256	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	0	4	from	effect	11:16	arg1	responses					45:53	inflammatory responses	32:53	inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways	32:132	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	7	5	theme	signal-regulated	1197:1212	arg1	kinase					1214:1219	extracellular signal-regulated kinase	1183:1219	extracellular signal-regulated kinase (ERK)	1183:1225	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	5	theme	signal-regulated	1197:1212	arg1	MAPK					1177:1180	p38 MAPK	1173:1180	p38 MAPK	1173:1180	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	5	theme	signal-regulated	1197:1212	arg1	ERK					1222:1224	ERK	1222:1224	ERK	1222:1224	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	9	6	theme	cytokines	1467:1475	arg1	production					1430:1439	the production	1426:1439	the production of NO and proinflammatory cytokines	1426:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	6	theme	cytokines	1467:1475	arg1	degradation					1541:1551	degradation	1541:1551	degradation of IκB	1541:1558	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	6	theme	cytokines	1467:1475	arg1	phosphorylation					1498:1512	phosphorylation	1498:1512	phosphorylation of NF-κB p65 and MAPK	1498:1534	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	6	theme	cytokines	1467:1475	arg1	expression					1478:1487	expression	1478:1487	expression of iNOS	1478:1495	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	11	7	theme	upstream	1898:1905	arg1	factor					1907:1912	its upstream factor	1894:1912	its upstream factor	1894:1912	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	1	8	theme	2'-hydroxy-3'-methacryloxypropoxy	244:276	arg1	propane					288:294	2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane	233:294	2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA)	233:303	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	1	8	theme	2'-hydroxy-3'-methacryloxypropoxy	244:276	arg1	BisGMA					297:302	BisGMA	297:302	BisGMA	297:302	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	9	9	theme	iNOS	1492:1495	arg1	production					1430:1439	the production	1426:1439	the production of NO and proinflammatory cytokines	1426:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	9	theme	iNOS	1492:1495	arg1	degradation					1541:1551	degradation	1541:1551	degradation of IκB	1541:1558	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	9	theme	iNOS	1492:1495	arg1	phosphorylation					1498:1512	phosphorylation	1498:1512	phosphorylation of NF-κB p65 and MAPK	1498:1534	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	9	theme	iNOS	1492:1495	arg1	expression					1478:1487	expression	1478:1487	expression of iNOS	1478:1495	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	7	10	theme	BisGMA-induced	1086:1099	arg1	phosphorylation					1101:1115	the BisGMA-induced phosphorylation	1082:1115	the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK)	1082:1256	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	6	11	theme	IκB	1040:1042	arg1	degradation					1045:1055	inhibitor of κB (IκB) degradation	1023:1055	inhibitor of κB (IκB) degradation	1023:1055	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	5	12	theme	proinflammatory	838:852	arg1	interleukin					905:915	interleukin	905:915	interleukin (IL)-1β	905:923	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	5	12	theme	proinflammatory	838:852	arg1	cytokines					854:862	proinflammatory cytokines	838:862	proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6	838:933	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	5	12	theme	proinflammatory	838:852	arg1	IL-6					930:933	IL-6	930:933	IL-6	930:933	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	5	12	theme	proinflammatory	838:852	arg1	factor					889:894	tumor necrosis factor (TNF)-α	874:902	tumor necrosis factor (TNF)-α	874:902	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	0	13	from	responses	45:53	arg1	macrophages					73:83	BisGMA-treated macrophages	58:83	BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways	58:132	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	9	14	located	observed	1414:1421	arg1	degradation					1541:1551	degradation	1541:1551	degradation of IκB	1541:1558	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	14	located	observed	1414:1421	arg1	production					1430:1439	the production	1426:1439	the production of NO and proinflammatory cytokines	1426:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	14	located	observed	1414:1421	arg1	phosphorylation					1498:1512	phosphorylation	1498:1512	phosphorylation of NF-κB p65 and MAPK	1498:1534	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	14	located	observed	1414:1421	arg2	effects					1390:1396	Similar inhibitory effects	1371:1396	Similar inhibitory effects of wogonin	1371:1407	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	14	located	observed	1414:1421	arg1	expression					1478:1487	expression	1478:1487	expression of iNOS	1478:1495	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	10	15	theme	potential	1601:1609	arg1	agent					1629:1633	a potential anti-inflammatory agent	1599:1633	a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation	1599:1760	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	10	15	theme	potential	1601:1609	arg1	rutin					1590:1594	rutin	1590:1594	rutin	1590:1594	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	9	16	theme	Similar	1371:1377	arg1	effects					1390:1396	Similar inhibitory effects	1371:1396	Similar inhibitory effects of wogonin	1371:1407	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	5	17	theme	necrosis	880:887	arg1	TNF					897:899	TNF	897:899	TNF	897:899	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	5	17	theme	necrosis	880:887	arg1	factor					889:894	tumor necrosis factor (TNF)-α	874:902	tumor necrosis factor (TNF)-α	874:902	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	6	18	theme	κB	1036:1037	arg1	degradation					1045:1055	inhibitor of κB (IκB) degradation	1023:1055	inhibitor of κB (IκB) degradation	1023:1055	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	0	19	theme	MAPK	111:114	arg1	inhibition					97:106	the inhibition	93:106	the inhibition of MAPK and NFκB pathways	93:132	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	2	20	theme	flavonoid	471:479	arg1	which					441:445	which	441:445	which	441:445	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	2	20	theme	flavonoid	471:479	arg1	compound					481:488	a natural dihydroxyl flavonoid compound	450:488	a natural dihydroxyl flavonoid compound	450:488	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	7	21	theme	kinases	1146:1152	arg1	phosphorylation					1101:1115	the BisGMA-induced phosphorylation	1082:1115	the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK)	1082:1256	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	8	22	from	macrophages	1322:1332	arg1	manner					1363:1368	a concentration-dependent manner	1337:1368	a concentration-dependent manner	1337:1368	All these effects were induced by wogonin in the macrophages in a concentration-dependent manner.
36178853	5	23	theme	tumor	874:878	arg1	TNF					897:899	TNF	897:899	TNF	897:899	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	5	23	theme	tumor	874:878	arg1	factor					889:894	tumor necrosis factor (TNF)-α	874:902	tumor necrosis factor (TNF)-α	874:902	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	7	24	theme	protein	1138:1144	arg1	MAPK					1177:1180	p38 MAPK	1173:1180	p38 MAPK	1173:1180	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	24	theme	protein	1138:1144	arg1	MAPKs					1155:1159	MAPKs	1155:1159	MAPKs	1155:1159	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	24	theme	protein	1138:1144	arg1	kinases					1146:1152	mitogen-activated protein kinases	1120:1152	mitogen-activated protein kinases (MAPKs)	1120:1160	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	10	25	theme	BisGMA-treated	1639:1652	arg1	macrophages					1654:1664	BisGMA-treated macrophages	1639:1664	BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation	1639:1760	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	7	26	theme	N-terminal	1234:1243	arg1	JNK					1253:1255	JNK	1253:1255	JNK	1253:1255	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	26	theme	N-terminal	1234:1243	arg1	MAPK					1177:1180	p38 MAPK	1173:1180	p38 MAPK	1173:1180	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	26	theme	N-terminal	1234:1243	arg1	kinase					1245:1250	c-Jun N-terminal kinase	1228:1250	c-Jun N-terminal kinase (JNK)	1228:1256	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	10	27	theme	p65	1684:1686	arg1	phosphorylation					1688:1702	NFκB p65 phosphorylation	1679:1702	NFκB p65 phosphorylation	1679:1702	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	6	28	theme	inhibitor	1023:1031	arg1	degradation					1045:1055	inhibitor of κB (IκB) degradation	1023:1055	inhibitor of κB (IκB) degradation	1023:1055	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	4	29	theme	oxide	666:670	arg1	generation					645:654	The generation	641:654	The generation of nitric oxide (NO)	641:675	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	4	29	theme	oxide	666:670	arg1	expression					685:694	the expression	681:694	the expression of inducible nitric oxide synthase (iNOS)	681:736	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	0	30	theme	Protective	0:9	arg1	effect					11:16	Protective effect	0:16	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.	0:133	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	1	31	theme	phenylene	278:286	arg1	propane					288:294	2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane	233:294	2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA)	233:303	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	1	31	theme	phenylene	278:286	arg1	BisGMA					297:302	BisGMA	297:302	BisGMA	297:302	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	9	32	theme	IκB	1556:1558	arg1	production					1430:1439	the production	1426:1439	the production of NO and proinflammatory cytokines	1426:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	32	theme	IκB	1556:1558	arg1	degradation					1541:1551	degradation	1541:1551	degradation of IκB	1541:1558	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	32	theme	IκB	1556:1558	arg1	phosphorylation					1498:1512	phosphorylation	1498:1512	phosphorylation of NF-κB p65 and MAPK	1498:1534	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	32	theme	IκB	1556:1558	arg1	expression					1478:1487	expression	1478:1487	expression of iNOS	1478:1495	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	3	33	theme	antiallergic	585:596	arg1	properties					629:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	According to the findings, wogonin exhibits anti-inflammatory, antiallergic, anticancer, and antioxidative properties.
36178853	6	34	theme	BisGMA-induced	961:974	arg1	factor					984:989	BisGMA-induced nuclear factor	961:989	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	6	34	theme	BisGMA-induced	961:974	arg1	NF					992:993	NF	992:993	NF	992:993	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	9	35	theme	wogonin	1401:1407	arg1	effects					1390:1396	Similar inhibitory effects	1371:1396	Similar inhibitory effects of wogonin	1371:1407	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	0	36	theme	wogonin	21:27	arg1	effect					11:16	Protective effect	0:16	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.	0:133	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	0	37	theme	pathways	125:132	arg1	inhibition					97:106	the inhibition	93:106	the inhibition of MAPK and NFκB pathways	93:132	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	3	38	theme	anticancer	599:608	arg1	properties					629:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	According to the findings, wogonin exhibits anti-inflammatory, antiallergic, anticancer, and antioxidative properties.
36178853	6	39	theme	-κB	995:997	arg1	phosphorylation					1003:1017	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	11	40	theme	pathway	1882:1888	arg1	regulation					1859:1868	the regulation	1855:1868	the regulation of the NFκB pathway and its upstream factor, MAPK	1855:1918	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	10	41	theme	anti-inflammatory	1611:1627	arg1	agent					1629:1633	a potential anti-inflammatory agent	1599:1633	a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation	1599:1760	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	10	41	theme	anti-inflammatory	1611:1627	arg1	rutin					1590:1594	rutin	1590:1594	rutin	1590:1594	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	2	42	theme	wogonin	432:438	arg1	responses					419:427	the anti-inflammatory responses	397:427	the anti-inflammatory responses of wogonin	397:438	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	11	43	theme	MAPK	1915:1918	arg1	regulation					1859:1868	the regulation	1855:1868	the regulation of the NFκB pathway and its upstream factor, MAPK	1855:1918	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	0	44	theme	NFκB	120:123	arg1	pathways					125:132	NFκB pathways	120:132	NFκB pathways	120:132	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	3	45	theme	anti-inflammatory	566:582	arg1	properties					629:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	According to the findings, wogonin exhibits anti-inflammatory, antiallergic, anticancer, and antioxidative properties.
36178853	10	46	theme	IκB	1708:1710	arg1	degradation					1712:1722	IκB degradation	1708:1722	IκB degradation	1708:1722	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	6	47	theme	p65	999:1001	arg1	phosphorylation					1003:1017	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	0	48	theme	inflammatory	32:43	arg1	responses					45:53	inflammatory responses	32:53	inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways	32:132	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	7	49	theme	c-Jun	1228:1232	arg1	JNK					1253:1255	JNK	1253:1255	JNK	1253:1255	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	49	theme	c-Jun	1228:1232	arg1	MAPK					1177:1180	p38 MAPK	1173:1180	p38 MAPK	1173:1180	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	49	theme	c-Jun	1228:1232	arg1	kinase					1245:1250	c-Jun N-terminal kinase	1228:1250	c-Jun N-terminal kinase (JNK)	1228:1256	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	2	50	from	compound	481:488	arg1	macrophages					509:519	BisGMA-treated macrophages	494:519	BisGMA-treated macrophages	494:519	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	9	51	theme	MAPK	1531:1534	arg1	production					1430:1439	the production	1426:1439	the production of NO and proinflammatory cytokines	1426:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	51	theme	MAPK	1531:1534	arg1	degradation					1541:1551	degradation	1541:1551	degradation of IκB	1541:1558	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	51	theme	MAPK	1531:1534	arg1	phosphorylation					1498:1512	phosphorylation	1498:1512	phosphorylation of NF-κB p65 and MAPK	1498:1534	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	51	theme	MAPK	1531:1534	arg1	expression					1478:1487	expression	1478:1487	expression of iNOS	1478:1495	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	11	52	theme	NFκB	1877:1880	arg1	pathway					1882:1888	the NFκB pathway	1873:1888	the NFκB pathway	1873:1888	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	2	53	theme	anti-inflammatory	401:417	arg1	responses					419:427	the anti-inflammatory responses	397:427	the anti-inflammatory responses of wogonin	397:438	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	2	54	theme	dihydroxyl	460:469	arg1	which					441:445	which	441:445	which	441:445	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	2	54	theme	dihydroxyl	460:469	arg1	compound					481:488	a natural dihydroxyl flavonoid compound	450:488	a natural dihydroxyl flavonoid compound	450:488	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	11	55	theme	proinflammatory	1806:1820	arg1	responses					1822:1830	BisGMA-induced proinflammatory responses	1791:1830	BisGMA-induced proinflammatory responses in macrophages	1791:1845	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	4	56	theme	nitric	659:664	arg1	NO					673:674	NO	673:674	NO	673:674	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	4	56	theme	nitric	659:664	arg1	oxide					666:670	nitric oxide	659:670	nitric oxide (NO)	659:675	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	2	57	theme	natural	452:458	arg1	which					441:445	which	441:445	which	441:445	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	2	57	theme	natural	452:458	arg1	compound					481:488	a natural dihydroxyl flavonoid compound	450:488	a natural dihydroxyl flavonoid compound	450:488	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	9	58	theme	inhibitory	1379:1388	arg1	effects					1390:1396	Similar inhibitory effects	1371:1396	Similar inhibitory effects of wogonin	1371:1407	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	4	59	theme	oxide	716:720	arg1	iNOS					732:735	iNOS	732:735	iNOS	732:735	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	4	59	theme	oxide	716:720	arg1	synthase					722:729	inducible nitric oxide synthase	699:729	inducible nitric oxide synthase (iNOS)	699:736	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	10	60	theme	NFκB	1679:1682	arg1	phosphorylation					1688:1702	NFκB p65 phosphorylation	1679:1702	NFκB p65 phosphorylation	1679:1702	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	11	61	from	responses	1822:1830	arg1	macrophages					1835:1845	macrophages	1835:1845	macrophages	1835:1845	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	1	62	used	used	186:189	arg2	sealants					159:166	sealants	159:166	sealants	159:166	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	1	62	used	used	186:189	arg2	resins					147:152	resins	147:152	resins	147:152	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	1	62	used	used	186:189	arg2	Composites					135:144	Composites	135:144	Composites	135:144	Composites, resins, and sealants that are commonly used in orthopedics and dentistry are based on 2,2-bis[p-(2'-hydroxy-3'-methacryloxypropoxy)phenylene]propane (BisGMA), which induces proinflammatory responses in macrophages.
36178853	4	63	theme	nitric	709:714	arg1	iNOS					732:735	iNOS	732:735	iNOS	732:735	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	4	63	theme	nitric	709:714	arg1	synthase					722:729	inducible nitric oxide synthase	699:729	inducible nitric oxide synthase (iNOS)	699:736	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	11	64	theme	BisGMA-induced	1791:1804	arg1	responses					1822:1830	BisGMA-induced proinflammatory responses	1791:1830	BisGMA-induced proinflammatory responses in macrophages	1791:1845	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	11	65	theme	factor	1907:1912	arg1	regulation					1859:1868	the regulation	1855:1868	the regulation of the NFκB pathway and its upstream factor, MAPK	1855:1918	Therefore, wogonin inhibits BisGMA-induced proinflammatory responses in macrophages through the regulation of the NFκB pathway and its upstream factor, MAPK.
36178853	10	66	theme	MAPK	1741:1744	arg1	phosphorylation					1746:1760	upstream MAPK phosphorylation	1732:1760	upstream MAPK phosphorylation	1732:1760	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	9	67	theme	NO	1444:1445	arg1	production					1430:1439	the production	1426:1439	the production of NO and proinflammatory cytokines	1426:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	67	theme	NO	1444:1445	arg1	degradation					1541:1551	degradation	1541:1551	degradation of IκB	1541:1558	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	67	theme	NO	1444:1445	arg1	phosphorylation					1498:1512	phosphorylation	1498:1512	phosphorylation of NF-κB p65 and MAPK	1498:1534	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	67	theme	NO	1444:1445	arg1	expression					1478:1487	expression	1478:1487	expression of iNOS	1478:1495	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	68	theme	p65	1523:1525	arg1	production					1430:1439	the production	1426:1439	the production of NO and proinflammatory cytokines	1426:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	68	theme	p65	1523:1525	arg1	degradation					1541:1551	degradation	1541:1551	degradation of IκB	1541:1558	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	68	theme	p65	1523:1525	arg1	phosphorylation					1498:1512	phosphorylation	1498:1512	phosphorylation of NF-κB p65 and MAPK	1498:1534	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	9	68	theme	p65	1523:1525	arg1	expression					1478:1487	expression	1478:1487	expression of iNOS	1478:1495	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	8	69	theme	concentration-dependent	1339:1361	arg1	manner					1363:1368	a concentration-dependent manner	1337:1368	a concentration-dependent manner	1337:1368	All these effects were induced by wogonin in the macrophages in a concentration-dependent manner.
36178853	4	70	theme	synthase	722:729	arg1	generation					645:654	The generation	641:654	The generation of nitric oxide (NO)	641:675	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	4	70	theme	synthase	722:729	arg1	expression					685:694	the expression	681:694	the expression of inducible nitric oxide synthase (iNOS)	681:736	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	9	71	theme	NF-κB	1517:1521	arg1	p65					1523:1525	NF-κB p65	1517:1525	NF-κB p65	1517:1525	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	10	72	theme	upstream	1732:1739	arg1	phosphorylation					1746:1760	upstream MAPK phosphorylation	1732:1760	upstream MAPK phosphorylation	1732:1760	These results indicated that rutin is a potential anti-inflammatory agent for BisGMA-treated macrophages that undergo NFκB p65 phosphorylation and IκB degradation through upstream MAPK phosphorylation.
36178853	2	73	theme	BisGMA-treated	494:507	arg1	macrophages					509:519	BisGMA-treated macrophages	494:519	BisGMA-treated macrophages	494:519	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	5	74	theme	cytokines	854:862	arg1	production					824:833	the production	820:833	the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6	820:933	Furthermore, the production of proinflammatory cytokines including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 was reduced.
36178853	3	75	theme	antioxidative	615:627	arg1	properties					629:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	anti-inflammatory, antiallergic, anticancer, and antioxidative properties	566:638	According to the findings, wogonin exhibits anti-inflammatory, antiallergic, anticancer, and antioxidative properties.
36178853	9	76	theme	proinflammatory	1451:1465	arg1	cytokines					1467:1475	proinflammatory cytokines	1451:1475	proinflammatory cytokines	1451:1475	Similar inhibitory effects of wogonin were observed on the production of NO and proinflammatory cytokines, expression of iNOS, phosphorylation of NF-κB p65 and MAPK, and degradation of IκB.
36178853	2	77	theme	present	366:372	arg1	study					374:378	The present study	362:378	The present study	362:378	The present study aimed to explore the anti-inflammatory responses of wogonin, which is a natural dihydroxyl flavonoid compound, in BisGMA-treated macrophages.
36178853	6	78	theme	of	1033:1034	arg1	degradation					1045:1055	inhibitor of κB (IκB) degradation	1023:1055	inhibitor of κB (IκB) degradation	1023:1055	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	6	79	theme	factor	984:989	arg1	phosphorylation					1003:1017	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	0	80	theme	BisGMA-treated	58:71	arg1	macrophages					73:83	BisGMA-treated macrophages	58:83	BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways	58:132	Protective effect of wogonin on inflammatory responses in BisGMA-treated macrophages through the inhibition of MAPK and NFκB pathways.
36178853	4	81	theme	BisGMA-treated	779:792	arg1	macrophages					794:804	BisGMA-treated macrophages	779:804	BisGMA-treated macrophages	779:804	The generation of nitric oxide (NO) and the expression of inducible nitric oxide synthase (iNOS) were noted to be inhibited by wogonin in BisGMA-treated macrophages.
36178853	7	82	theme	mitogen-activated	1120:1136	arg1	MAPK					1177:1180	p38 MAPK	1173:1180	p38 MAPK	1173:1180	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	82	theme	mitogen-activated	1120:1136	arg1	MAPKs					1155:1159	MAPKs	1155:1159	MAPKs	1155:1159	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	82	theme	mitogen-activated	1120:1136	arg1	kinases					1146:1152	mitogen-activated protein kinases	1120:1152	mitogen-activated protein kinases (MAPKs)	1120:1160	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	83	theme	extracellular	1183:1195	arg1	kinase					1214:1219	extracellular signal-regulated kinase	1183:1219	extracellular signal-regulated kinase (ERK)	1183:1225	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	83	theme	extracellular	1183:1195	arg1	MAPK					1177:1180	p38 MAPK	1173:1180	p38 MAPK	1173:1180	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	7	83	theme	extracellular	1183:1195	arg1	ERK					1222:1224	ERK	1222:1224	ERK	1222:1224	Finally, the BisGMA-induced phosphorylation of mitogen-activated protein kinases (MAPKs), including p38 MAPK, extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) was inhibited.
36178853	6	84	theme	nuclear	976:982	arg1	factor					984:989	BisGMA-induced nuclear factor	961:989	BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation	961:1017	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36178853	6	84	theme	nuclear	976:982	arg1	NF					992:993	NF	992:993	NF	992:993	In addition, BisGMA-induced nuclear factor (NF)-κB p65 phosphorylation and inhibitor of κB (IκB) degradation were inhibited.
36744901	8	0	attach	removed	1090:1096	arg1	supernatant					1115:1125	the culture supernatant	1103:1125	the culture supernatant	1103:1125	When MVs were removed from the culture supernatant by ultracentrifugation, 54 of the secretome proteins were significantly depleted in abundance.
36744901	8	0	attach	removed	1090:1096	arg2	MVs					1081:1083	MVs	1081:1083	MVs	1081:1083	When MVs were removed from the culture supernatant by ultracentrifugation, 54 of the secretome proteins were significantly depleted in abundance.
36744901	11	1	dep	IMPORTANCE	1543:1552	arg1	secretome					1558:1566	The secretome	1554:1566	IMPORTANCE The secretome of Staphylococcus aureus	1543:1591	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	7	2	dep	secretome	1057:1065	arg1	the					1053:1055	the	1053:1055	the	1053:1055	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	7	2	dep	secretome	1057:1065	arg1	both					1045:1048	both	1045:1048	both	1045:1048	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	11	3	theme	extracellular	1635:1647	arg1	vesicles					1658:1665	nano-sized extracellular membrane vesicles	1624:1665	nano-sized extracellular membrane vesicles (MVs)	1624:1671	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	11	3	theme	extracellular	1635:1647	arg1	MVs					1668:1670	MVs	1668:1670	MVs	1668:1670	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	6	4	theme	Cell	780:783	arg1	proteins					805:812	Cell membrane-associated proteins	780:812	Cell membrane-associated proteins	780:812	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	2	5	theme	wall-associated	349:363	arg1	proteins					365:372	some cell wall-associated proteins	339:372	some cell wall-associated proteins	339:372	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	2	6	theme	membrane	296:303	arg1	proteins					305:312	cytoplasmic and membrane proteins	280:312	proteins	305:312	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	10	7	theme	aureus	1405:1410	arg1	MVs					1412:1414	S. aureus MVs	1402:1414	S. aureus MVs	1402:1414	Our data indicate that the generation of S. aureus MVs is a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome.
36744901	2	8	theme	cytoplasmic	280:290	arg1	proteins					305:312	cytoplasmic and membrane proteins	280:312	proteins	305:312	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	8	9	theme	secretome	1161:1169	arg1	proteins					1171:1178	the secretome proteins	1157:1178	the secretome proteins	1157:1178	When MVs were removed from the culture supernatant by ultracentrifugation, 54 of the secretome proteins were significantly depleted in abundance.
36744901	6	10	from	acid	831:834	arg1	supernatant					851:861	the culture supernatant	839:861	the culture supernatant	839:861	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	5	11	attach	present	764:770	arg2	667					750:752	667	750:752	667	750:752	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	5	11	attach	present	764:770	arg1	MVs					775:777	MVs	775:777	MVs	775:777	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	16	12	theme	MVs	2330:2332	arg1	contribution					2314:2325	the contribution	2310:2325	the contribution of MVs to the secretome of S. aureus	2310:2362	This study deepens our limited understanding about the contribution of MVs to the secretome of S. aureus.
36744901	4	13	theme	secretome	619:627	arg1	supernatant					638:648	the whole secretome (culture supernatant)	609:649	the whole secretome (culture supernatant)	609:649	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	1	14	theme	microbial	95:103	arg1	secretome					105:113	The microbial secretome	91:113	The microbial secretome	91:113	The microbial secretome modulates how the organism interacts with its environment.
36744901	4	15	theme	culture	630:636	arg1	supernatant					638:648	the whole secretome (culture supernatant)	609:649	the whole secretome (culture supernatant)	609:649	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	16	16	theme	aureus	2357:2362	arg1	secretome					2341:2349	the secretome	2337:2349	the secretome of S. aureus	2337:2362	This study deepens our limited understanding about the contribution of MVs to the secretome of S. aureus.
36744901	4	17	dep	along	576:580	arg1	with					582:585	with	582:585	with	582:585	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	11	18	theme	membrane	1649:1656	arg1	vesicles					1658:1665	nano-sized extracellular membrane vesicles	1624:1665	nano-sized extracellular membrane vesicles (MVs)	1624:1671	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	11	18	theme	membrane	1649:1656	arg1	MVs					1668:1670	MVs	1668:1670	MVs	1668:1670	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	2	19	theme	membrane	240:247	arg1	Included					174:181	Included	174:181	Included	174:181	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	2	19	theme	membrane	240:247	arg1	MVs					259:261	MVs	259:261	MVs	259:261	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	2	19	theme	membrane	240:247	arg1	vesicles					249:256	extracellular membrane vesicles	226:256	extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers	226:391	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	12	20	theme	secretome	1710:1718	arg1	composition					1686:1696	The protein composition	1674:1696	The protein composition of both the secretome and MVs	1674:1726	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	12	21	theme	MVs	1724:1726	arg1	composition					1686:1696	The protein composition	1674:1696	The protein composition of both the secretome and MVs	1674:1726	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	15	22	from	abundance	2156:2164	arg1	secretome					2173:2181	the secretome	2169:2181	the secretome	2169:2181	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	7	23	theme	wall-associated	957:971	arg1	proteins					973:980	Few cell wall-associated proteins	948:980	Few cell wall-associated proteins	948:980	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	4	24	theme	MVs	681:683	arg1	depletion					668:676	depletion	668:676	depletion of MVs	668:683	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	0	25	theme	aureus	83:88	arg1	Secretome					55:63	the Secretome	51:63	the Secretome of Staphylococcus aureus	51:88	Contribution of Extracellular Membrane Vesicles To the Secretome of Staphylococcus aureus.
36744901	7	26	theme	Few	948:950	arg1	proteins					973:980	Few cell wall-associated proteins	948:980	Few cell wall-associated proteins	948:980	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	4	27	theme	proteomic	507:515	arg1	analysis					517:524	a proteomic analysis	505:524	a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant)	505:649	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	15	28	from	association	2187:2197	arg1	secretome					2173:2181	the secretome	2169:2181	the secretome	2169:2181	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	13	29	theme	MV	1859:1860	arg1	cargo					1862:1866	the MV cargo	1855:1866	the MV cargo	1855:1866	How the MV cargo differs from the protein composition of the secretome has not yet been addressed.
36744901	3	30	theme	diverse	436:442	arg1	composition					444:454	the diverse composition	432:454	the diverse composition of the secretome	432:471	The extent to which MVs contribute to the diverse composition of the secretome is not understood.
36744901	5	31	theme	1,001	725:729	arg1	proteins					731:738	1,001 proteins	725:738	1,001 proteins	725:738	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	5	32	located	present	764:770	arg1	MVs					775:777	MVs	775:777	MVs	775:777	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	5	32	located	present	764:770	arg2	667					750:752	667	750:752	667	750:752	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	14	33	theme	secretome	1983:1991	arg1	similar					2017:2023	similar	2017:2023	similar	2017:2023	Although the compositions of the secretome and MVs were strikingly similar, we identified 54 proteins that were specifically packaged in MVs.
36744901	14	33	theme	secretome	1983:1991	arg1	MVs					1997:1999	MVs	1997:1999	MVs	1997:1999	Although the compositions of the secretome and MVs were strikingly similar, we identified 54 proteins that were specifically packaged in MVs.
36744901	14	33	theme	secretome	1983:1991	arg1	compositions					1963:1974	the compositions	1959:1974	the compositions of the secretome	1959:1991	Although the compositions of the secretome and MVs were strikingly similar, we identified 54 proteins that were specifically packaged in MVs.
36744901	3	34	theme	secretome	463:471	arg1	composition					444:454	the diverse composition	432:454	the diverse composition of the secretome	432:471	The extent to which MVs contribute to the diverse composition of the secretome is not understood.
36744901	0	35	theme	Extracellular	16:28	arg1	Vesicles					39:46	Extracellular Membrane Vesicles	16:46	Extracellular Membrane Vesicles	16:46	Contribution of Extracellular Membrane Vesicles To the Secretome of Staphylococcus aureus.
36744901	15	36	theme	isoelectric	2240:2250	arg1	point					2252:2256	a basic isoelectric point	2232:2256	a basic isoelectric point	2232:2256	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	6	37	theme	culture	843:849	arg1	supernatant					851:861	the culture supernatant	839:861	the culture supernatant	839:861	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	4	38	theme	aureus	554:559	arg1	strain					561:566	the S. aureus strain MRSA252	547:574	the S. aureus strain MRSA252	547:574	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	0	39	theme	Vesicles	39:46	arg1	Contribution					0:11	Contribution	0:11	Contribution of Extracellular Membrane Vesicles To the Secretome of Staphylococcus aureus.	0:89	Contribution of Extracellular Membrane Vesicles To the Secretome of Staphylococcus aureus.
36744901	12	40	theme	wall-associated	1806:1820	arg1	proteins					1822:1829	some cell wall-associated proteins	1796:1829	some cell wall-associated proteins	1796:1829	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	2	41	theme	cell	344:347	arg1	proteins					365:372	some cell wall-associated proteins	339:372	some cell wall-associated proteins	339:372	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	6	42	theme	lipoteichoic	818:829	arg1	acid					831:834	lipoteichoic acid	818:834	lipoteichoic acid in the culture supernatant	818:861	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	10	43	theme	membrane-associated	1481:1499	arg1	proteins					1501:1508	cell membrane-associated proteins	1476:1508	cell membrane-associated proteins	1476:1508	Our data indicate that the generation of S. aureus MVs is a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome.
36744901	4	44	theme	similar	589:595	arg1	analysis					597:604	a similar analysis	587:604	a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant)	505:649	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	2	45	theme	aureus	205:210	arg1	secretome					212:220	the Staphylococcus aureus secretome	186:220	the Staphylococcus aureus secretome	186:220	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	11	46	theme	nano-sized	1624:1633	arg1	vesicles					1658:1665	nano-sized extracellular membrane vesicles	1624:1665	nano-sized extracellular membrane vesicles (MVs)	1624:1671	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	11	46	theme	nano-sized	1624:1633	arg1	MVs					1668:1670	MVs	1668:1670	MVs	1668:1670	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	9	47	theme	isoelectric	1272:1282	arg1	point					1284:1288	an isoelectric point	1269:1288	an isoelectric point that was significantly higher than that of proteins excluded from MVs	1269:1358	Proteins packaged in MVs were characterized by an isoelectric point that was significantly higher than that of proteins excluded from MVs.
36744901	9	47	theme	isoelectric	1272:1282	arg1	higher					1313:1318	higher	1313:1318	higher	1313:1318	Proteins packaged in MVs were characterized by an isoelectric point that was significantly higher than that of proteins excluded from MVs.
36744901	10	48	theme	S.	1402:1403	arg1	MVs					1412:1414	S. aureus MVs	1402:1414	S. aureus MVs	1402:1414	Our data indicate that the generation of S. aureus MVs is a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome.
36744901	15	49	from	point	2252:2256	arg1	secretome					2173:2181	the secretome	2169:2181	the secretome	2169:2181	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	12	50	theme	cytoplasmic	1737:1747	arg1	proteins					1762:1769	cytoplasmic and membrane proteins	1737:1769	proteins	1762:1769	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	4	51	theme	supernatant	638:648	arg1	analysis					597:604	a similar analysis	587:604	a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant)	505:649	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	15	52	with	point	2252:2256	arg1	membrane					2218:2225	the bacterial membrane	2204:2225	the bacterial membrane	2204:2225	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	12	53	theme	membrane	1753:1760	arg1	proteins					1762:1769	cytoplasmic and membrane proteins	1737:1769	proteins	1762:1769	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	12	54	dep	secretome	1710:1718	arg1	the					1706:1708	the	1706:1708	the	1706:1708	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	8	55	theme	proteins	1171:1178	arg1	proteins					1171:1178	the secretome proteins	1157:1178	the secretome proteins	1157:1178	When MVs were removed from the culture supernatant by ultracentrifugation, 54 of the secretome proteins were significantly depleted in abundance.
36744901	8	55	theme	proteins	1171:1178	arg1	54					1151:1152	54	1151:1152	54	1151:1152	When MVs were removed from the culture supernatant by ultracentrifugation, 54 of the secretome proteins were significantly depleted in abundance.
36744901	4	56	theme	whole	613:617	arg1	supernatant					638:648	the whole secretome (culture supernatant)	609:649	the whole secretome (culture supernatant)	609:649	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	6	57	from	proteins	805:812	arg1	supernatant					851:861	the culture supernatant	839:861	the culture supernatant	839:861	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	5	58	theme	MRSA252	690:696	arg1	secretome					698:706	The MRSA252 secretome	686:706	The MRSA252 secretome	686:706	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	6	59	theme	extracellular	924:936	arg1	proteins					938:945	cytoplasmic and extracellular proteins	908:945	cytoplasmic and extracellular proteins	908:945	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	15	60	theme	bacterial	2208:2216	arg1	membrane					2218:2225	the bacterial membrane	2204:2225	the bacterial membrane	2204:2225	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	6	61	theme	cytoplasmic	908:918	arg1	proteins					938:945	cytoplasmic and extracellular proteins	908:945	cytoplasmic and extracellular proteins	908:945	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	7	62	theme	capsular	1009:1016	arg1	polysaccharides					1018:1032	capsular polysaccharides	1009:1032	capsular polysaccharides	1009:1032	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	13	63	theme	secretome	1912:1920	arg1	composition					1893:1903	the protein composition	1881:1903	the protein composition of the secretome	1881:1920	How the MV cargo differs from the protein composition of the secretome has not yet been addressed.
36744901	16	64	theme	limited	2282:2288	arg1	understanding					2290:2302	our limited understanding	2278:2302	our limited understanding about the contribution of MVs to the secretome of S. aureus	2278:2362	This study deepens our limited understanding about the contribution of MVs to the secretome of S. aureus.
36744901	5	65	theme	proteins	731:738	arg1	present					764:770	present	764:770	present	764:770	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	5	66	from	present	764:770	arg1	MVs					775:777	MVs	775:777	MVs	775:777	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	12	67	theme	protein	1678:1684	arg1	composition					1686:1696	The protein composition	1674:1696	The protein composition of both the secretome and MVs	1674:1726	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	10	68	theme	MVs	1412:1414	arg1	mechanism					1421:1429	a mechanism	1419:1429	a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome	1419:1540	Our data indicate that the generation of S. aureus MVs is a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome.
36744901	10	68	theme	MVs	1412:1414	arg1	generation					1388:1397	the generation	1384:1397	the generation of S. aureus MVs	1384:1414	Our data indicate that the generation of S. aureus MVs is a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome.
36744901	13	69	theme	protein	1885:1891	arg1	composition					1893:1903	the protein composition	1881:1903	the protein composition of the secretome	1881:1920	How the MV cargo differs from the protein composition of the secretome has not yet been addressed.
36744901	7	70	theme	cell	952:955	arg1	proteins					973:980	Few cell wall-associated proteins	948:980	Few cell wall-associated proteins	948:980	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	15	71	with	association	2187:2197	arg1	membrane					2218:2225	the bacterial membrane	2204:2225	the bacterial membrane	2204:2225	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	7	72	located	found	1039:1043	arg1	MVs					1071:1073	MVs	1071:1073	MVs	1071:1073	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	7	72	located	found	1039:1043	arg2	polysaccharides					1018:1032	capsular polysaccharides	1009:1032	capsular polysaccharides	1009:1032	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	7	72	located	found	1039:1043	arg1	secretome					1057:1065	secretome	1057:1065	secretome	1057:1065	Few cell wall-associated proteins were contained in MVs, and capsular polysaccharides were found both in the secretome and MVs.
36744901	10	73	theme	lipoteichoic	1440:1451	arg1	acid					1453:1456	lipoteichoic acid	1440:1456	lipoteichoic acid	1440:1456	Our data indicate that the generation of S. aureus MVs is a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome.
36744901	5	74	from	MVs	775:777	arg1	present					764:770	present	764:770	present	764:770	The MRSA252 secretome was comprised of 1,001 proteins, of which 667 were also present in MVs.
36744901	4	75	theme	S.	551:552	arg1	strain					561:566	the S. aureus strain MRSA252	547:574	the S. aureus strain MRSA252	547:574	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	0	76	theme	Membrane	30:37	arg1	Vesicles					39:46	Extracellular Membrane Vesicles	16:46	Extracellular Membrane Vesicles	16:46	Contribution of Extracellular Membrane Vesicles To the Secretome of Staphylococcus aureus.
36744901	2	77	theme	Staphylococcus	190:203	arg1	secretome					212:220	the Staphylococcus aureus secretome	186:220	the Staphylococcus aureus secretome	186:220	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	4	78	theme	MVs	529:531	arg1	analysis					517:524	a proteomic analysis	505:524	a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant)	505:649	We performed a proteomic analysis of MVs purified from the S. aureus strain MRSA252 along with a similar analysis of the whole secretome (culture supernatant) before and after depletion of MVs.
36744901	11	79	theme	aureus	1586:1591	arg1	secretome					1558:1566	The secretome	1554:1566	IMPORTANCE The secretome of Staphylococcus aureus	1543:1591	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
36744901	15	80	theme	basic	2234:2238	arg1	point					2252:2256	a basic isoelectric point	2232:2256	a basic isoelectric point	2232:2256	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	12	81	theme	cell	1801:1804	arg1	proteins					1822:1829	some cell wall-associated proteins	1796:1829	some cell wall-associated proteins	1796:1829	The protein composition of both the secretome and MVs includes cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	15	82	with	abundance	2156:2164	arg1	membrane					2218:2225	the bacterial membrane	2204:2225	the bacterial membrane	2204:2225	Proteins highly associated with MVs were characterized by their abundance in the secretome, an association with the bacterial membrane, and a basic isoelectric point.
36744901	2	83	theme	extracellular	226:238	arg1	Included					174:181	Included	174:181	Included	174:181	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	2	83	theme	extracellular	226:238	arg1	MVs					259:261	MVs	259:261	MVs	259:261	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	2	83	theme	extracellular	226:238	arg1	vesicles					249:256	extracellular membrane vesicles	226:256	extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers	226:391	Included in the Staphylococcus aureus secretome are extracellular membrane vesicles (MVs) that consist of cytoplasmic and membrane proteins, as well as exoproteins, some cell wall-associated proteins, and glycopolymers.
36744901	10	84	theme	cell	1476:1479	arg1	proteins					1501:1508	cell membrane-associated proteins	1476:1508	cell membrane-associated proteins	1476:1508	Our data indicate that the generation of S. aureus MVs is a mechanism by which lipoteichoic acid, cytoplasmic, and cell membrane-associated proteins are released into the secretome.
36744901	8	85	theme	culture	1107:1113	arg1	supernatant					1115:1125	the culture supernatant	1103:1125	the culture supernatant	1103:1125	When MVs were removed from the culture supernatant by ultracentrifugation, 54 of the secretome proteins were significantly depleted in abundance.
36744901	6	86	theme	membrane-associated	785:803	arg1	proteins					805:812	Cell membrane-associated proteins	780:812	Cell membrane-associated proteins	780:812	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	6	87	dep	associated	875:884	arg1	followed					896:903	followed	896:903	followed by cytoplasmic and extracellular proteins	896:945	Cell membrane-associated proteins and lipoteichoic acid in the culture supernatant were highly associated with MVs, followed by cytoplasmic and extracellular proteins.
36744901	11	88	theme	soluble	1602:1608	arg1	molecules					1610:1618	soluble molecules	1602:1618	soluble molecules	1602:1618	IMPORTANCE The secretome of Staphylococcus aureus includes soluble molecules and nano-sized extracellular membrane vesicles (MVs).
37173037	8	0	theme	gut	1336:1338	arg1	composition					1351:1361	gut microbiota composition	1336:1361	gut microbiota composition in mice	1336:1369	Moreover, the formulations could alter gut microbiota composition in mice.
37173037	10	1	theme	biocompatibility	1579:1594	arg1	multi-bioresponsiveness					1597:1619	excellent biocompatibility, multi-bioresponsiveness, and colon targeting	1569:1640	multi-bioresponsiveness	1597:1619	These PS-loaded bilayer microgels, exhibiting excellent biocompatibility, multi-bioresponsiveness, and colon targeting, could be beneficial in UC therapy, allowing development into a novel oral formulation.
37173037	4	2	theme	multifunctional	834:848	arg1	system					859:864	a multifunctional delivery system	832:864	a multifunctional delivery system	832:864	The porous starch (PS)-mediated strong adsorption allowed Cur loading in inner core to achieve a multifunctional delivery system.
37173037	1	3	theme	bilayer	146:152	arg1	microgels					154:162	bilayer microgels	146:162	bilayer microgels	146:162	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	10	4	theme	excellent	1569:1577	arg1	multi-bioresponsiveness					1597:1619	excellent biocompatibility, multi-bioresponsiveness, and colon targeting	1569:1640	multi-bioresponsiveness	1597:1619	These PS-loaded bilayer microgels, exhibiting excellent biocompatibility, multi-bioresponsiveness, and colon targeting, could be beneficial in UC therapy, allowing development into a novel oral formulation.
37173037	1	5	theme	microgels	154:162	arg1	type					138:141	one type	134:141	one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation	134:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	7	6	theme	colonic	1281:1287	arg1	tissue					1289:1294	colonic tissue	1281:1294	colonic tissue	1281:1294	The formulations facilitated colonic delivery, allowing Cur accumulation in colonic tissue.
37173037	5	7	theme	pH	935:936	arg1	conditions					938:947	different pH conditions	925:947	different pH conditions	925:947	In vitro, the formulations exhibited good bioresponses at different pH conditions, potentially delaying Cur release in the upper gastrointestinal tract.
37173037	1	8	theme	colon	241:245	arg1	effects					199:205	three effects	193:205	three effects: pH responsiveness, time lag, and colon enzyme degradation	193:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	8	theme	colon	241:245	arg1	degradation					254:264	colon enzyme degradation	241:264	colon enzyme degradation	241:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	10	9	theme	colon	1626:1630	arg1	targeting					1632:1640	excellent biocompatibility, multi-bioresponsiveness, and colon targeting	1569:1640	targeting	1632:1640	These PS-loaded bilayer microgels, exhibiting excellent biocompatibility, multi-bioresponsiveness, and colon targeting, could be beneficial in UC therapy, allowing development into a novel oral formulation.
37173037	4	10	theme	delivery	850:857	arg1	system					859:864	a multifunctional delivery system	832:864	a multifunctional delivery system	832:864	The porous starch (PS)-mediated strong adsorption allowed Cur loading in inner core to achieve a multifunctional delivery system.
37173037	8	11	theme	microbiota	1340:1349	arg1	composition					1351:1361	gut microbiota composition	1336:1361	gut microbiota composition in mice	1336:1369	Moreover, the formulations could alter gut microbiota composition in mice.
37173037	1	12	theme	enzyme	247:252	arg1	effects					199:205	three effects	193:205	three effects: pH responsiveness, time lag, and colon enzyme degradation	193:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	12	theme	enzyme	247:252	arg1	degradation					254:264	colon enzyme degradation	241:264	colon enzyme degradation	241:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	0	13	theme	colitis	113:119	arg1	delivery					76:83	controlled drug delivery	60:83	controlled drug delivery	60:83	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	0	13	theme	colitis	113:119	arg1	treatment					89:97	treatment	89:97	treatment of ulcerative colitis	89:119	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	7	14	from	accumulation	1265:1276	arg1	tissue					1289:1294	colonic tissue	1281:1294	colonic tissue	1281:1294	The formulations facilitated colonic delivery, allowing Cur accumulation in colonic tissue.
37173037	3	15	theme	inner	511:515	arg1	core					517:520	The inner core	507:520	The inner core	507:520	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	6	16	theme	colitis	1071:1077	arg1	symptoms					1084:1091	dextran sulfate sodium-induced ulcerative colitis (UC) symptoms	1029:1091	dextran sulfate sodium-induced ulcerative colitis (UC) symptoms	1029:1091	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	0	17	theme	ulcerative	102:111	arg1	colitis					113:119	ulcerative colitis	102:119	ulcerative colitis	102:119	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	3	18	theme	polyelectrolyte	677:691	arg1	interaction					693:703	polyelectrolyte interaction	677:703	polyelectrolyte interaction	677:703	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	4	19	theme	inner	810:814	arg1	core					816:819	inner core	810:819	inner core	810:819	The porous starch (PS)-mediated strong adsorption allowed Cur loading in inner core to achieve a multifunctional delivery system.
37173037	1	20	theme	oral	168:171	arg1	administration					173:186	oral administration	168:186	oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation	168:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	7	21	theme	colonic	1234:1240	arg1	delivery					1242:1249	colonic delivery	1234:1249	colonic delivery	1234:1249	The formulations facilitated colonic delivery, allowing Cur accumulation in colonic tissue.
37173037	5	22	from	release	975:981	arg1	tract					1013:1017	the upper gastrointestinal tract	986:1017	the upper gastrointestinal tract	986:1017	In vitro, the formulations exhibited good bioresponses at different pH conditions, potentially delaying Cur release in the upper gastrointestinal tract.
37173037	6	23	theme	sodium-induced	1045:1058	arg1	UC					1080:1081	UC	1080:1081	UC	1080:1081	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	6	23	theme	sodium-induced	1045:1058	arg1	colitis					1071:1077	dextran sulfate sodium-induced ulcerative colitis	1029:1077	dextran sulfate sodium-induced ulcerative colitis (UC) symptoms	1029:1091	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	0	24	theme	Oral	0:3	arg1	delivery					5:12	Oral delivery	0:12	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.	0:120	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	2	25	theme	biological	290:299	arg1	effects					301:307	the dual biological effects	281:307	the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury	281:398	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	3	26	attach	derived	523:529	arg1	gum					541:543	guar gum	536:543	guar gum	536:543	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	3	26	attach	derived	523:529	arg1	pectin					562:567	low-methoxyl pectin	549:567	low-methoxyl pectin	549:567	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	3	26	attach	derived	523:529	arg2	core					517:520	The inner core	507:520	The inner core	507:520	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	6	27	theme	sulfate	1037:1043	arg1	UC					1080:1081	UC	1080:1081	UC	1080:1081	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	6	27	theme	sulfate	1037:1043	arg1	colitis					1071:1077	dextran sulfate sodium-induced ulcerative colitis	1029:1077	dextran sulfate sodium-induced ulcerative colitis (UC) symptoms	1029:1091	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	0	28	theme	porous	17:22	arg1	microgels					46:54	porous starch-loaded bilayer microgels	17:54	porous starch-loaded bilayer microgels	17:54	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	6	29	theme	factors	1196:1202	arg1	levels					1173:1178	reduced levels	1165:1178	reduced levels of inflammatory factors	1165:1202	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	10	30	theme	PS-loaded	1529:1537	arg1	microgels					1547:1555	These PS-loaded bilayer microgels	1523:1555	These PS-loaded bilayer microgels	1523:1555	These PS-loaded bilayer microgels, exhibiting excellent biocompatibility, multi-bioresponsiveness, and colon targeting, could be beneficial in UC therapy, allowing development into a novel oral formulation.
37173037	9	31	theme	bacterial	1459:1467	arg1	content					1469:1475	pathogenic bacterial content	1448:1475	pathogenic bacterial content	1448:1475	During Cur delivery, each formulation increased species richness, decreased pathogenic bacterial content, and afforded synergistic effects against UC.
37173037	2	32	theme	dual	285:288	arg1	effects					301:307	the dual biological effects	281:307	the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury	281:398	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	5	33	theme	upper	990:994	arg1	tract					1013:1017	the upper gastrointestinal tract	986:1017	the upper gastrointestinal tract	986:1017	In vitro, the formulations exhibited good bioresponses at different pH conditions, potentially delaying Cur release in the upper gastrointestinal tract.
37173037	6	34	theme	dextran	1029:1035	arg1	sulfate					1037:1043	dextran sulfate	1029:1043	dextran sulfate sodium-induced ulcerative colitis (UC) symptoms	1029:1091	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	2	35	theme	targeted	401:408	arg1	localization					418:429	targeted colonic localization	401:429	targeted colonic localization	401:429	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	3	36	theme	colonic	715:721	arg1	localization					723:734	colonic localization	715:734	colonic localization	715:734	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	6	37	theme	reduced	1165:1171	arg1	levels					1173:1178	reduced levels	1165:1178	reduced levels of inflammatory factors	1165:1202	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	3	38	theme	guar	536:539	arg1	gum					541:543	guar gum	536:543	guar gum	536:543	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	9	39	theme	pathogenic	1448:1457	arg1	content					1469:1475	pathogenic bacterial content	1448:1475	pathogenic bacterial content	1448:1475	During Cur delivery, each formulation increased species richness, decreased pathogenic bacterial content, and afforded synergistic effects against UC.
37173037	2	40	theme	injury	393:398	arg1	repair					367:372	repair	367:372	repair of colonic mucosal injury	367:398	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	0	41	theme	bilayer	38:44	arg1	microgels					46:54	porous starch-loaded bilayer microgels	17:54	porous starch-loaded bilayer microgels	17:54	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	3	42	theme	outer	626:630	arg1	layer					632:636	the outer layer	622:636	the outer layer	622:636	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	4	43	theme	Cur	795:797	arg1	loading					799:805	Cur loading	795:805	Cur loading	795:805	The porous starch (PS)-mediated strong adsorption allowed Cur loading in inner core to achieve a multifunctional delivery system.
37173037	6	44	theme	inflammatory	1183:1194	arg1	factors					1196:1202	inflammatory factors	1183:1202	inflammatory factors	1183:1202	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	2	45	theme	mucosal	385:391	arg1	injury					393:398	colonic mucosal injury	377:398	colonic mucosal injury	377:398	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	2	46	theme	Cur	446:448	arg1	localization					418:429	targeted colonic localization	401:429	targeted colonic localization	401:429	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	2	46	theme	Cur	446:448	arg1	release					435:441	release	435:441	release	435:441	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	0	47	theme	starch-loaded	24:36	arg1	microgels					46:54	porous starch-loaded bilayer microgels	17:54	porous starch-loaded bilayer microgels	17:54	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	2	48	theme	colonic	410:416	arg1	localization					418:429	targeted colonic localization	401:429	targeted colonic localization	401:429	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	1	49	theme	pH	208:209	arg1	effects					199:205	three effects	193:205	three effects: pH responsiveness, time lag, and colon enzyme degradation	193:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	49	theme	pH	208:209	arg1	responsiveness					211:224	pH responsiveness	208:224	pH responsiveness	208:224	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	9	50	theme	Cur	1379:1381	arg1	delivery					1383:1390	Cur delivery	1379:1390	Cur delivery	1379:1390	During Cur delivery, each formulation increased species richness, decreased pathogenic bacterial content, and afforded synergistic effects against UC.
37173037	1	51	with	administration	173:186	arg1	effects					199:205	three effects	193:205	three effects: pH responsiveness, time lag, and colon enzyme degradation	193:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	51	with	administration	173:186	arg1	responsiveness					211:224	pH responsiveness	208:224	pH responsiveness	208:224	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	51	with	administration	173:186	arg1	lag					232:234	time lag	227:234	time lag	227:234	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	51	with	administration	173:186	arg1	degradation					254:264	colon enzyme degradation	241:264	colon enzyme degradation	241:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	2	52	theme	colonic	467:473	arg1	microenvironment					475:490	the colonic microenvironment	463:490	the colonic microenvironment	463:490	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	3	53	theme	low-methoxyl	549:560	arg1	pectin					562:567	low-methoxyl pectin	549:567	low-methoxyl pectin	549:567	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	0	54	theme	microgels	46:54	arg1	delivery					5:12	Oral delivery	0:12	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.	0:120	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	10	55	theme	oral	1712:1715	arg1	formulation					1717:1727	a novel oral formulation	1704:1727	a novel oral formulation	1704:1727	These PS-loaded bilayer microgels, exhibiting excellent biocompatibility, multi-bioresponsiveness, and colon targeting, could be beneficial in UC therapy, allowing development into a novel oral formulation.
37173037	8	56	from	composition	1351:1361	arg1	mice					1366:1369	mice	1366:1369	mice	1366:1369	Moreover, the formulations could alter gut microbiota composition in mice.
37173037	9	57	theme	synergistic	1491:1501	arg1	effects					1503:1509	synergistic effects	1491:1509	synergistic effects	1491:1509	During Cur delivery, each formulation increased species richness, decreased pathogenic bacterial content, and afforded synergistic effects against UC.
37173037	4	58	theme	strong	769:774	arg1	adsorption					776:785	The porous starch (PS)-mediated strong adsorption	737:785	The porous starch (PS)-mediated strong adsorption	737:785	The porous starch (PS)-mediated strong adsorption allowed Cur loading in inner core to achieve a multifunctional delivery system.
37173037	5	59	theme	Cur	971:973	arg1	release					975:981	Cur release	971:981	Cur release in the upper gastrointestinal tract	971:1017	In vitro, the formulations exhibited good bioresponses at different pH conditions, potentially delaying Cur release in the upper gastrointestinal tract.
37173037	4	60	theme	-mediated	759:767	arg1	adsorption					776:785	The porous starch (PS)-mediated strong adsorption	737:785	The porous starch (PS)-mediated strong adsorption	737:785	The porous starch (PS)-mediated strong adsorption allowed Cur loading in inner core to achieve a multifunctional delivery system.
37173037	5	61	theme	gastrointestinal	996:1011	arg1	tract					1013:1017	the upper gastrointestinal tract	986:1017	the upper gastrointestinal tract	986:1017	In vitro, the formulations exhibited good bioresponses at different pH conditions, potentially delaying Cur release in the upper gastrointestinal tract.
37173037	2	62	theme	curcumin	312:319	arg1	effects					301:307	the dual biological effects	281:307	the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury	281:398	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	5	63	theme	different	925:933	arg1	conditions					938:947	different pH conditions	925:947	different pH conditions	925:947	In vitro, the formulations exhibited good bioresponses at different pH conditions, potentially delaying Cur release in the upper gastrointestinal tract.
37173037	10	64	theme	bilayer	1539:1545	arg1	microgels					1547:1555	These PS-loaded bilayer microgels	1523:1555	These PS-loaded bilayer microgels	1523:1555	These PS-loaded bilayer microgels, exhibiting excellent biocompatibility, multi-bioresponsiveness, and colon targeting, could be beneficial in UC therapy, allowing development into a novel oral formulation.
37173037	3	65	theme	degradation	600:610	arg1	behavior					612:619	degradation behavior	600:619	degradation behavior	600:619	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	10	66	theme	novel	1706:1710	arg1	formulation					1717:1727	a novel oral formulation	1704:1727	a novel oral formulation	1704:1727	These PS-loaded bilayer microgels, exhibiting excellent biocompatibility, multi-bioresponsiveness, and colon targeting, could be beneficial in UC therapy, allowing development into a novel oral formulation.
37173037	5	67	theme	good	904:907	arg1	bioresponses					909:920	good bioresponses	904:920	good bioresponses	904:920	In vitro, the formulations exhibited good bioresponses at different pH conditions, potentially delaying Cur release in the upper gastrointestinal tract.
37173037	0	68	theme	drug	71:74	arg1	delivery					76:83	controlled drug delivery	60:83	controlled drug delivery	60:83	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	2	69	theme	colonic	377:383	arg1	injury					393:398	colonic mucosal injury	377:398	colonic mucosal injury	377:398	Combined with the dual biological effects of curcumin (Cur) for reducing inflammation and promoting repair of colonic mucosal injury, targeted colonic localization and release of Cur according to the colonic microenvironment were enhanced.
37173037	3	70	theme	colonic	579:585	arg1	adhesion					587:594	colonic adhesion	579:594	colonic adhesion	579:594	The inner core, derived from guar gum and low-methoxyl pectin, afforded colonic adhesion and degradation behavior; the outer layer, modified by alginate and chitosan via polyelectrolyte interaction, achieved colonic localization.
37173037	7	71	theme	Cur	1261:1263	arg1	accumulation					1265:1276	Cur accumulation	1261:1276	Cur accumulation in colonic tissue	1261:1294	The formulations facilitated colonic delivery, allowing Cur accumulation in colonic tissue.
37173037	6	72	theme	ulcerative	1060:1069	arg1	UC					1080:1081	UC	1080:1081	UC	1080:1081	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	6	72	theme	ulcerative	1060:1069	arg1	colitis					1071:1077	dextran sulfate sodium-induced ulcerative colitis	1029:1077	dextran sulfate sodium-induced ulcerative colitis (UC) symptoms	1029:1091	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	1	73	theme	time	227:230	arg1	effects					199:205	three effects	193:205	three effects: pH responsiveness, time lag, and colon enzyme degradation	193:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	73	theme	time	227:230	arg1	lag					232:234	time lag	227:234	time lag	227:234	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	0	74	theme	controlled	60:69	arg1	delivery					76:83	controlled drug delivery	60:83	controlled drug delivery	60:83	Oral delivery of porous starch-loaded bilayer microgels for controlled drug delivery and treatment of ulcerative colitis.
37173037	6	75	theme	oral	1129:1132	arg1	administration					1134:1147	oral administration	1129:1147	oral administration	1129:1147	In vivo, dextran sulfate sodium-induced ulcerative colitis (UC) symptoms were significantly alleviated after oral administration, accompanied by reduced levels of inflammatory factors.
37173037	1	76	dep	effects	199:205	arg1	effects					199:205	three effects	193:205	three effects: pH responsiveness, time lag, and colon enzyme degradation	193:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	76	dep	effects	199:205	arg1	responsiveness					211:224	pH responsiveness	208:224	pH responsiveness	208:224	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	76	dep	effects	199:205	arg1	lag					232:234	time lag	227:234	time lag	227:234	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	1	76	dep	effects	199:205	arg1	degradation					254:264	colon enzyme degradation	241:264	colon enzyme degradation	241:264	We prepared one type of bilayer microgels for oral administration with three effects: pH responsiveness, time lag, and colon enzyme degradation.
37173037	9	77	theme	species	1420:1426	arg1	richness					1428:1435	species richness	1420:1435	species richness	1420:1435	During Cur delivery, each formulation increased species richness, decreased pathogenic bacterial content, and afforded synergistic effects against UC.
36931747	0	0	from	Anabolism	164:172	arg1	Rats					177:180	Rats	177:180	Rats	177:180	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	3	1	theme	protein	509:515	arg1	metabolisms					528:538	protein and energy metabolisms	509:538	protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context	509:635	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	1	2	theme	better	241:246	arg1	health					263:268	better cardiovascular health	241:268	better cardiovascular health than animal proteins (APs) in epidemiological studies	241:322	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	10	3	theme	lipids	1812:1817	arg1	routings					1774:1781	higher routings	1767:1781	higher routings of dietary carbohydrates and lipids	1767:1817	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	10	4	theme	AA	1838:1839	arg1	synthesis					1841:1849	dispensable AA synthesis	1826:1849	dispensable AA synthesis by glycolysis and β-oxidation	1826:1879	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	3	5	theme	dietary	551:557	arg1	sources					567:573	dietary protein sources	551:573	dietary protein sources (PP compared with AP)	551:595	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	3	5	theme	dietary	551:557	arg1	PP					576:577	PP	576:577	PP compared with AP	576:594	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	11	6	theme	metabolic	2082:2090	arg1	dysregulations					2092:2105	tissue metabolic dysregulations	2075:2105	tissue metabolic dysregulations	2075:2105	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	4	7	theme	Male	646:649	arg1	rats					658:661	Male Wistar rats	646:661	Male Wistar rats (n = 44, 8 wk old)	646:680	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	6	8	theme	macronutrient	1225:1237	arg1	routing					1239:1245	macronutrient routing	1225:1245	macronutrient routing to dispensable AA using a 13C natural abundance method	1225:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	1	9	theme	BACKGROUND	183:192	arg1	proteins					200:207	BACKGROUND Plant proteins	183:207	BACKGROUND Plant proteins (PPs)	183:213	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	1	9	theme	BACKGROUND	183:192	arg1	PPs					210:212	PPs	210:212	PPs	210:212	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	10	10	theme	tissue	1903:1908	arg1	PS					1910:1911	similar tissue PS and protein mass	1895:1928	PS	1910:1911	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	7	11	theme	Mixed	1441:1445	arg1	models					1447:1452	Mixed models	1441:1452	Mixed models	1441:1452	Data were analyzed using ANOVA and Mixed models.
36931747	1	12	from	health	263:268	arg1	studies					316:322	epidemiological studies	300:322	epidemiological studies	300:322	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	4	13	from	fat	896:898	arg1	normal					827:832	normal	827:832	normal	827:832	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	13	from	fat	896:898	arg1	NFS					835:837	NFS	835:837	NFS	835:837	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	6	14	dep	de	1358:1359	arg1	novo					1361:1364	novo	1361:1364	novo	1361:1364	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	9	15	theme	lipid	1609:1613	arg1	content					1615:1621	lower lipid content	1603:1621	lower lipid content (-17%, P < 0.01)	1603:1638	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	6	16	theme	lipogenesis	1366:1376	arg1	fluxes					1321:1326	the metabolic fluxes	1307:1326	the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis	1307:1376	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	16	theme	lipogenesis	1366:1376	arg1	method					1204:1209	a 15N natural abundance method	1180:1209	a 15N natural abundance method	1180:1209	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	16	theme	lipogenesis	1366:1376	arg1	rates					1216:1220	the rates	1212:1220	the rates of macronutrient routing to dispensable AA using a 13C natural abundance method	1212:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	11	17	theme	balanced	1980:1987	arg1	blend					1989:1993	a balanced blend	1978:1993	a balanced blend of PP	1978:1999	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	10	18	theme	carbohydrates	1794:1806	arg1	routings					1774:1781	higher routings	1767:1781	higher routings of dietary carbohydrates and lipids	1767:1817	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	6	19	theme	de	1358:1359	arg1	lipogenesis					1366:1376	de novo lipogenesis	1358:1376	de novo lipogenesis	1358:1376	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	9	20	theme	lower	1603:1607	arg1	content					1615:1621	lower lipid content	1603:1621	lower lipid content (-17%, P < 0.01)	1603:1638	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	4	21	theme	old	677:679	arg1	wk					674:675	8 wk	672:675	8 wk old	672:679	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	5	22	theme	plasma	1022:1027	arg1	metabolites					1029:1039	plasma metabolites	1022:1039	plasma metabolites	1022:1039	We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites.
36931747	0	23	theme	Protein	156:162	arg1	Anabolism					164:172	Tissue Protein Anabolism	149:172	Tissue Protein Anabolism in Rats	149:180	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	6	24	theme	natural	1277:1283	arg1	method					1295:1300	a 13C natural abundance method	1271:1300	a 13C natural abundance method	1271:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	11	25	theme	related	2126:2132	arg1	mechanisms					2115:2124	mechanisms	2115:2124	mechanisms related to their less optimal AA profile for direct channeling to PS	2115:2193	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	0	26	theme	Pea	40:42	arg1	Blend					52:56	a Wheat and Pea Protein Blend	28:56	Blend	52:56	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	0	27	theme	Milk	14:17	arg1	Protein					19:25	Milk Protein	14:25	Milk Protein	14:25	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	8	28	theme	whole-body	1470:1479	arg1	level					1481:1485	the whole-body level	1466:1485	the whole-body level	1466:1485	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	9	29	dep	lipogenesis	1652:1662	arg1	<					1673:1673	<	1673:1673	<	1673:1673	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	10	30	theme	AA	1734:1735	arg1	transamination					1737:1750	higher AA transamination	1727:1750	higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids	1727:1817	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	5	31	theme	lipid	1003:1007	arg1	content					1009:1015	lipid content	1003:1015	lipid content	1003:1015	We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites.
36931747	4	32	dep	PP	793:794	arg1	%					799:799	50%	797:799	50%:50% pea: wheat	797:814	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	9	33	dep	<	1673:1673	arg1	%					1668:1668	-24%	1665:1668	-24%	1665:1668	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	11	34	theme	protein	2020:2026	arg1	anabolism					2028:2036	protein anabolism	2020:2036	protein anabolism	2020:2036	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	11	35	theme	growing	1946:1952	arg1	rats					1954:1957	growing rats	1946:1957	growing rats	1946:1957	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	0	36	theme	High-Fat	66:73	arg1	Dysregulations					107:120	High-Fat, High-Sucrose Induced Metabolic Dysregulations	66:120	High-Fat, High-Sucrose Induced Metabolic Dysregulations	66:120	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	6	37	theme	synthesis	1339:1347	arg1	fluxes					1321:1326	the metabolic fluxes	1307:1326	the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis	1307:1376	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	37	theme	synthesis	1339:1347	arg1	method					1204:1209	a 15N natural abundance method	1180:1209	a 15N natural abundance method	1180:1209	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	37	theme	synthesis	1339:1347	arg1	rates					1216:1220	the rates	1212:1220	the rates of macronutrient routing to dispensable AA using a 13C natural abundance method	1212:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	8	38	dep	%	1534:1534	arg1	<					1569:1569	P < 0.05	1567:1574	P < 0.05	1567:1574	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	10	39	theme	higher	1767:1772	arg1	routings					1774:1781	higher routings	1767:1781	higher routings of dietary carbohydrates and lipids	1767:1817	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	4	40	theme	isoproteic	707:716	arg1	diets					718:722	isoproteic diets	707:722	isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively	707:929	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	3	41	theme	isotopic	440:447	arg1	methods					449:455	cutting-edge isotopic methods	427:455	cutting-edge isotopic methods	427:455	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	8	42	theme	insulin	1511:1517	arg1	resistance					1519:1528	HFS-induced insulin resistance	1499:1528	HFS-induced insulin resistance	1499:1528	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	0	43	dep	High-Fat	66:73	arg1	Induced					89:95	Induced	89:95	Induced	89:95	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	6	44	theme	labeling	1389:1396	arg1	method					1398:1403	a 2H labeling method	1384:1403	a 2H labeling method	1384:1403	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	0	45	theme	Protein	44:50	arg1	Blend					52:56	a Wheat and Pea Protein Blend	28:56	Blend	52:56	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	4	46	from	sucrose	857:863	arg1	normal					827:832	normal	827:832	normal	827:832	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	46	from	sucrose	857:863	arg1	NFS					835:837	NFS	835:837	NFS	835:837	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	6	47	theme	metabolic	1311:1319	arg1	fluxes					1321:1326	the metabolic fluxes	1307:1326	the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis	1307:1376	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	8	48	theme	HFS	1555:1557	arg1	groups					1559:1564	HFS groups	1555:1564	HFS groups	1555:1564	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	6	49	theme	adipose	1067:1073	arg1	tissues					1075:1081	adipose tissues	1067:1081	adipose tissues	1067:1081	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	9	50	theme	de	1644:1645	arg1	lipogenesis					1652:1662	de novo lipogenesis	1644:1662	de novo lipogenesis (-24%, P < 0.05)	1644:1679	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	6	51	theme	acid	1152:1155	arg1	trafficking					1162:1172	amino acid (AA) trafficking	1146:1172	amino acid (AA) trafficking	1146:1172	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	4	52	theme	isolate	751:757	arg1	sources					759:765	their protein isolate sources	737:765	their protein isolate sources	737:765	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	3	53	theme	dietary	621:627	arg1	context					629:635	a prudent or western dietary context	600:635	a prudent or western dietary context	600:635	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	11	54	from	CONCLUSIONS	1931:1941	arg1	rats					1954:1957	growing rats	1946:1957	growing rats	1946:1957	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	9	55	dep	<	1632:1632	arg1	%					1627:1627	-17%	1624:1627	-17%	1624:1627	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	6	56	theme	natural	1186:1192	arg1	method					1204:1209	a 15N natural abundance method	1180:1209	a 15N natural abundance method	1180:1209	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	5	57	theme	enzyme	981:986	arg1	activities					988:997	hepatic enzyme activities	973:997	hepatic enzyme activities	973:997	We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites.
36931747	4	58	dep	METHODS	638:644	arg1	fed					687:689	fed	687:689	were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively	682:929	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	59	from	normal	827:832	arg1	fat					896:898	saturated fat	886:898	saturated fat (7% or 20% kcal)	886:915	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	59	from	normal	827:832	arg1	sucrose					857:863	sucrose	857:863	sucrose (6% or 15% kcal)	857:880	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	11	60	theme	optimal	2148:2154	arg1	profile					2159:2165	their less optimal AA profile	2137:2165	their less optimal AA profile for direct channeling to PS	2137:2193	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	2	61	theme	underlying	338:347	arg1	mechanisms					359:368	the underlying metabolic mechanisms	334:368	the underlying metabolic mechanisms	334:368	However, the underlying metabolic mechanisms remain mostly unknown.
36931747	9	62	dep	de	1644:1645	arg1	novo					1647:1650	novo	1647:1650	novo	1647:1650	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	5	63	theme	body	944:947	arg1	weight					949:954	body weight	944:954	body weight	944:954	We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites.
36931747	4	64	theme	%	782:782	arg1	milk					784:787	100% milk	779:787	100% milk	779:787	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	64	theme	%	782:782	arg1	AP					775:776	either AP	768:776	either AP (100% milk)	768:788	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	6	65	theme	trafficking	1162:1172	arg1	extent					1136:1141	the extent	1132:1141	the extent of amino acid (AA) trafficking	1132:1172	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	1	66	theme	cardiovascular	248:261	arg1	health					263:268	better cardiovascular health	241:268	better cardiovascular health than animal proteins (APs) in epidemiological studies	241:322	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	10	67	theme	dispensable	1826:1836	arg1	synthesis					1841:1849	dispensable AA synthesis	1826:1849	dispensable AA synthesis by glycolysis and β-oxidation	1826:1879	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	11	68	theme	direct	2171:2176	arg1	channeling					2178:2187	direct channeling	2171:2187	direct channeling to PS	2171:2193	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	4	69	dep	sucrose	857:863	arg1	%					867:867	6%	866:867	6%	866:867	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	69	dep	sucrose	857:863	arg1	%					874:874	15% kcal	872:879	15% kcal	872:879	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	6	70	theme	dispensable	1250:1260	arg1	AA					1262:1263	dispensable AA	1250:1263	dispensable AA using a 13C natural abundance method	1250:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	71	theme	routing	1239:1245	arg1	fluxes					1321:1326	the metabolic fluxes	1307:1326	the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis	1307:1376	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	71	theme	routing	1239:1245	arg1	method					1204:1209	a 15N natural abundance method	1180:1209	a 15N natural abundance method	1180:1209	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	71	theme	routing	1239:1245	arg1	rates					1216:1220	the rates	1212:1220	the rates of macronutrient routing to dispensable AA using a 13C natural abundance method	1212:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	4	72	theme	saturated	886:894	arg1	fat					896:898	saturated fat	886:898	saturated fat (7% or 20% kcal)	886:915	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	73	theme	Wistar	651:656	arg1	rats					658:661	Male Wistar rats	646:661	Male Wistar rats (n = 44, 8 wk old)	646:680	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	3	74	from	differences	494:504	arg1	metabolisms					528:538	protein and energy metabolisms	509:538	protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context	509:635	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	3	75	theme	protein	559:565	arg1	sources					567:573	dietary protein sources	551:573	dietary protein sources (PP compared with AP)	551:595	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	3	75	theme	protein	559:565	arg1	PP					576:577	PP	576:577	PP compared with AP	576:594	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	1	76	theme	Plant	194:198	arg1	proteins					200:207	BACKGROUND Plant proteins	183:207	BACKGROUND Plant proteins (PPs)	183:213	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	1	76	theme	Plant	194:198	arg1	PPs					210:212	PPs	210:212	PPs	210:212	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	10	77	theme	similar	1895:1901	arg1	PS					1910:1911	similar tissue PS and protein mass	1895:1928	PS	1910:1911	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	6	78	theme	2H	1386:1387	arg1	method					1398:1403	a 2H labeling method	1384:1403	a 2H labeling method	1384:1403	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	10	79	theme	different	1689:1697	arg1	tissues					1699:1705	the different tissues	1685:1705	the different tissues studied	1685:1713	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	8	80	dep	limited	1491:1497	arg1	%					1534:1534	-27%	1531:1534	-27% in HOMA-IR between HFS groups	1531:1564	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	4	81	dep	diets	718:722	arg1	normal					827:832	normal	827:832	normal	827:832	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	81	dep	diets	718:722	arg1	differing					724:732	differing	724:732	differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat)	724:815	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	81	dep	diets	718:722	arg1	NFS					835:837	NFS	835:837	NFS	835:837	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	82	theme	%	803:803	arg1	pea					805:807	50% pea	801:807	50%:50% pea: wheat	797:814	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	11	83	theme	tissue	2075:2080	arg1	dysregulations					2092:2105	tissue metabolic dysregulations	2075:2105	tissue metabolic dysregulations	2075:2105	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	10	84	theme	higher	1727:1732	arg1	transamination					1737:1750	higher AA transamination	1727:1750	higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids	1727:1817	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	0	85	theme	Tissue	149:154	arg1	Anabolism					164:172	Tissue Protein Anabolism	149:172	Tissue Protein Anabolism in Rats	149:180	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	4	86	dep	fat	896:898	arg1	%					902:902	7%	901:902	7%	901:902	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	86	dep	fat	896:898	arg1	%					909:909	20% kcal	907:914	20% kcal	907:914	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	10	87	theme	protein	1917:1923	arg1	mass					1925:1928	similar tissue PS and protein mass	1895:1928	mass	1925:1928	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	3	88	theme	prudent	602:608	arg1	context					629:635	a prudent or western dietary context	600:635	a prudent or western dietary context	600:635	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	6	89	theme	abundance	1285:1293	arg1	method					1295:1300	a 13C natural abundance method	1271:1300	a 13C natural abundance method	1271:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	4	90	theme	kcal	911:914	arg1	%					909:909	20% kcal	907:914	20% kcal	907:914	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	91	dep	%	799:799	arg1	wheat					810:814	wheat	810:814	50%:50% pea: wheat	797:814	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	4	91	dep	%	799:799	arg1	pea					805:807	50% pea	801:807	50%:50% pea: wheat	797:814	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	6	92	theme	13C	1273:1275	arg1	method					1295:1300	a 13C natural abundance method	1271:1300	a 13C natural abundance method	1271:1300	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	93	theme	skeletal	1088:1095	arg1	muscles					1097:1103	skeletal muscles	1088:1103	skeletal muscles	1088:1103	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	8	94	theme	P	1567:1567	arg1	<					1569:1569	P < 0.05	1567:1574	P < 0.05	1567:1574	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	10	95	theme	dietary	1786:1792	arg1	carbohydrates					1794:1806	dietary carbohydrates	1786:1806	dietary carbohydrates	1786:1806	In the different tissues studied, PP induced higher AA transamination accompanied by higher routings of dietary carbohydrates and lipids toward dispensable AA synthesis by glycolysis and β-oxidation, resulting in similar tissue PS and protein mass.
36931747	4	96	theme	=	666:666	arg1	n					664:664	n = 44	664:669	n = 44	664:669	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	6	97	theme	protein	1331:1337	arg1	synthesis					1339:1347	protein synthesis	1331:1347	protein synthesis (PS)	1331:1352	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	97	theme	protein	1331:1337	arg1	PS					1350:1351	PS	1350:1351	PS	1350:1351	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	4	98	dep	n	664:664	arg1	wk					674:675	8 wk	672:675	8 wk old	672:679	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	3	99	theme	methods	449:455	arg1	combination					412:422	a combination	410:422	a combination of cutting-edge isotopic methods	410:455	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	8	100	theme	HFS-induced	1499:1509	arg1	resistance					1519:1528	HFS-induced insulin resistance	1499:1528	HFS-induced insulin resistance	1499:1528	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	3	101	theme	cutting-edge	427:438	arg1	methods					449:455	cutting-edge isotopic methods	427:455	cutting-edge isotopic methods	427:455	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	1	102	theme	animal	275:280	arg1	proteins					282:289	animal proteins	275:289	animal proteins (APs)	275:295	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	1	102	theme	animal	275:280	arg1	APs					292:294	APs	292:294	APs	292:294	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	8	103	from	%	1534:1534	arg1	HOMA-IR					1539:1545	HOMA-IR	1539:1545	HOMA-IR	1539:1545	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	9	104	dep	content	1615:1621	arg1	<					1632:1632	<	1632:1632	<	1632:1632	In the liver, PP induced lower lipid content (-17%, P < 0.01) and de novo lipogenesis (-24%, P < 0.05).
36931747	6	105	theme	amino	1146:1150	arg1	AA					1158:1159	AA	1158:1159	AA	1158:1159	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	6	105	theme	amino	1146:1150	arg1	acid					1152:1155	amino acid	1146:1155	amino acid (AA) trafficking	1146:1172	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	8	106	dep	RESULTS	1455:1461	arg1	limited					1491:1497	limited	1491:1497	limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05)	1491:1575	RESULTS At the whole-body level, PP limited HFS-induced insulin resistance (-27% in HOMA-IR between HFS groups, P < 0.05).
36931747	3	107	theme	western	613:619	arg1	context					629:635	a prudent or western dietary context	600:635	a prudent or western dietary context	600:635	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	4	108	theme	protein	743:749	arg1	sources					759:765	their protein isolate sources	737:765	their protein isolate sources	737:765	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	1	109	theme	epidemiological	300:314	arg1	studies					316:322	epidemiological studies	300:322	epidemiological studies	300:322	BACKGROUND Plant proteins (PPs) have been associated with better cardiovascular health than animal proteins (APs) in epidemiological studies.
36931747	4	110	dep	rats	658:661	arg1	n					664:664	n = 44	664:669	n = 44	664:669	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	11	111	theme	AA	2156:2157	arg1	profile					2159:2165	their less optimal AA profile	2137:2165	their less optimal AA profile for direct channeling to PS	2137:2193	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	3	112	theme	energy	521:526	arg1	metabolisms					528:538	protein and energy metabolisms	509:538	protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context	509:635	OBJECTIVES Using a combination of cutting-edge isotopic methods, we aimed to better characterize the differences in protein and energy metabolisms induced by dietary protein sources (PP compared with AP) in a prudent or western dietary context.
36931747	6	113	theme	abundance	1194:1202	arg1	method					1204:1209	a 15N natural abundance method	1180:1209	a 15N natural abundance method	1180:1209	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36931747	5	114	theme	hepatic	973:979	arg1	activities					988:997	hepatic enzyme activities	973:997	hepatic enzyme activities	973:997	We measured body weight and composition, hepatic enzyme activities and lipid content, and plasma metabolites.
36931747	11	115	theme	PP	1998:1999	arg1	blend					1989:1993	a balanced blend	1978:1993	a balanced blend of PP	1978:1999	CONCLUSIONS In growing rats, compared with AP, a balanced blend of PP similarly supports protein anabolism while better limiting whole-body and tissue metabolic dysregulations through mechanisms related to their less optimal AA profile for direct channeling to PS.
36931747	0	116	theme	Metabolic	97:105	arg1	Dysregulations					107:120	High-Fat, High-Sucrose Induced Metabolic Dysregulations	66:120	High-Fat, High-Sucrose Induced Metabolic Dysregulations	66:120	Compared with Milk Protein, a Wheat and Pea Protein Blend Reduces High-Fat, High-Sucrose Induced Metabolic Dysregulations while Similarly Supporting Tissue Protein Anabolism in Rats.
36931747	4	117	theme	kcal	876:879	arg1	%					874:874	15% kcal	872:879	15% kcal	872:879	METHODS Male Wistar rats (n = 44, 8 wk old) were fed for 4.5 mo with isoproteic diets differing in their protein isolate sources, either AP (100% milk) or PP (50%:50% pea: wheat) and being normal (NFS) or high (HFS) in sucrose (6% or 15% kcal) and saturated fat (7% or 20% kcal), respectively.
36931747	2	118	theme	metabolic	349:357	arg1	mechanisms					359:368	the underlying metabolic mechanisms	334:368	the underlying metabolic mechanisms	334:368	However, the underlying metabolic mechanisms remain mostly unknown.
36931747	6	119	theme	15N	1182:1184	arg1	method					1204:1209	a 15N natural abundance method	1180:1209	a 15N natural abundance method	1180:1209	In the intestine, liver, adipose tissues, and skeletal muscles, we concomitantly assessed the extent of amino acid (AA) trafficking using a 15N natural abundance method, the rates of macronutrient routing to dispensable AA using a 13C natural abundance method, and the metabolic fluxes of protein synthesis (PS) and de novo lipogenesis using a 2H labeling method.
36136578	4	0	theme	bovine	847:852	arg1	MAC-T					884:888	MAC-T	884:888	MAC-T	884:888	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	4	0	theme	bovine	847:852	arg1	line					878:881	a bovine mammary epithelial cell line	845:881	a bovine mammary epithelial cell line (MAC-T)	845:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	6	1	theme	dextran	1136:1142	arg1	flux					1116:1119	paracellular flux	1103:1119	paracellular flux of FITC-40 kDa dextran	1103:1142	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	11	2	from	studies	2115:2121	arg1	effects					2139:2145	the combined effects	2126:2145	the combined effects of the two mycotoxins	2126:2167	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	6	3	theme	FITC-40	1124:1130	arg1	kDa					1132:1134	kDa	1132:1134	kDa	1132:1134	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	4	4	theme	mammary	820:826	arg1	epithelium					828:837	the bovine mammary epithelium	809:837	the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T)	809:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	7	5	theme	receptor	1324:1331	arg1	expression					1275:1284	expression	1275:1284	expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines	1275:1354	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	3	6	theme	bovine	543:548	arg1	epithelium					558:567	The bovine mammary epithelium	539:567	The bovine mammary epithelium	539:567	The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder.
36136578	7	7	theme	tight-junction	1289:1302	arg1	proteins					1304:1311	tight-junction proteins	1289:1311	tight-junction proteins	1289:1311	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	1	8	theme	ruminant	196:203	arg1	feed					205:208	ruminant feed	196:208	ruminant feed	196:208	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	4	9	theme	epithelial	862:871	arg1	MAC-T					884:888	MAC-T	884:888	MAC-T	884:888	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	4	9	theme	epithelial	862:871	arg1	line					878:881	a bovine mammary epithelial cell line	845:881	a bovine mammary epithelial cell line (MAC-T)	845:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	7	10	theme	expression	1275:1284	arg1	assessment					1261:1270	assessment	1261:1270	assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines	1261:1354	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	10	11	theme	innate	1914:1919	arg1	functions					1928:1936	barrier and innate immune functions	1902:1936	functions	1928:1936	These results suggest that OTA and CIT could potentially differentially modulate barrier and innate immune functions of mammary epithelium.
36136578	4	12	theme	present	691:697	arg1	study					699:703	The present study	687:703	The present study	687:703	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	4	12	theme	present	691:697	arg1	first					712:716	first	712:716	first	712:716	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	6	13	theme	48	1223:1224	arg1	h					1226:1226	h	1226:1226	h	1226:1226	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	5	14	theme	concentration-dependent	989:1011	arg1	manner					1013:1018	a concentration-dependent manner	987:1018	a concentration-dependent manner	987:1018	Results indicated that OTA and CIT exposure for 48 h significantly decreased cell viability in a concentration-dependent manner (p &lt; 0.05).
36136578	10	15	theme	barrier	1902:1908	arg1	functions					1928:1936	barrier and innate immune functions	1902:1936	functions	1928:1936	These results suggest that OTA and CIT could potentially differentially modulate barrier and innate immune functions of mammary epithelium.
36136578	1	16	theme	mycotoxins	144:153	arg1	detection					131:139	Frequent detection	122:139	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff	122:222	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	16	theme	mycotoxins	144:153	arg1	threat					243:248	a potential threat	231:248	a potential threat to feed safety, animal performance and health	231:294	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	11	17	theme	present	1965:1971	arg1	study					1973:1977	The present study	1961:1977	The present study	1961:1977	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	10	18	theme	mammary	1941:1947	arg1	epithelium					1949:1958	mammary epithelium	1941:1958	mammary epithelium	1941:1958	These results suggest that OTA and CIT could potentially differentially modulate barrier and innate immune functions of mammary epithelium.
36136578	0	19	theme	Epithelial	65:74	arg1	Permeability					81:92	Bovine Mammary Epithelial Cell Permeability	50:92	Bovine Mammary Epithelial Cell Permeability	50:92	Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.
36136578	11	20	from	toxicity	2019:2026	arg1	epithelium					2064:2073	bovine mammary epithelium	2049:2073	bovine mammary epithelium	2049:2073	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	2	21	theme	biodistribution	502:516	arg1	result					486:491	the result	482:491	the result of their biodistribution throughout the body	482:536	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	0	22	theme	Innate	98:103	arg1	Function					112:119	Innate Immune Function	98:119	Innate Immune Function	98:119	Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.
36136578	4	23	from	effects	744:750	arg1	functions					796:804	barrier and innate immune functions	770:804	functions	796:804	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	5	24	theme	CIT	923:925	arg1	exposure					927:934	OTA and CIT exposure	915:934	OTA and CIT exposure for 48 h	915:943	Results indicated that OTA and CIT exposure for 48 h significantly decreased cell viability in a concentration-dependent manner (p &lt; 0.05).
36136578	11	25	theme	individual	2008:2017	arg1	toxicity					2019:2026	the individual toxicity	2004:2026	the individual toxicity of each mycotoxin on bovine mammary epithelium	2004:2073	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	11	26	theme	future	2108:2113	arg1	studies					2115:2121	future studies	2108:2121	future studies on the combined effects of the two mycotoxins	2108:2167	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	4	27	theme	OTA	755:757	arg1	effects					744:750	individual effects	733:750	individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T)	733:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	0	28	theme	Ochratoxin	0:9	arg1	A					11:11	Ochratoxin A	0:11	Ochratoxin A	0:11	Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.
36136578	11	29	theme	combined	2130:2137	arg1	effects					2139:2145	the combined effects	2126:2145	the combined effects of the two mycotoxins	2126:2167	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	6	30	from	increase	1091:1098	arg1	flux					1116:1119	paracellular flux	1103:1119	paracellular flux of FITC-40 kDa dextran	1103:1142	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	6	30	from	increase	1091:1098	arg1	resistance					1076:1085	transepithelial electrical resistance	1049:1085	transepithelial electrical resistance	1049:1085	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	5	31	theme	OTA	915:917	arg1	exposure					927:934	OTA and CIT exposure	915:934	OTA and CIT exposure for 48 h	915:943	Results indicated that OTA and CIT exposure for 48 h significantly decreased cell viability in a concentration-dependent manner (p &lt; 0.05).
36136578	4	32	theme	innate	782:787	arg1	functions					796:804	barrier and innate immune functions	770:804	functions	796:804	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	1	33	theme	animal	266:271	arg1	performance					273:283	animal performance	266:283	animal performance	266:283	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	0	34	theme	Bovine	50:55	arg1	Permeability					81:92	Bovine Mammary Epithelial Cell Permeability	50:92	Bovine Mammary Epithelial Cell Permeability	50:92	Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.
36136578	4	35	theme	CIT	763:765	arg1	effects					744:750	individual effects	733:750	individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T)	733:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	2	36	theme	Ineffective	297:307	arg1	biodegradation					309:322	Ineffective biodegradation	297:322	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds	297:404	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	6	37	theme	transepithelial	1049:1063	arg1	resistance					1076:1085	transepithelial electrical resistance	1049:1085	transepithelial electrical resistance	1049:1085	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	11	38	theme	bovine	2049:2054	arg1	epithelium					2064:2073	bovine mammary epithelium	2049:2073	bovine mammary epithelium	2049:2073	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	8	39	theme	p	1468:1468	arg1	occludin					1458:1465	occludin	1458:1465	occludin (p &lt; 0.05)	1458:1479	Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p &lt; 0.05), whereas CIT did not affect zonula occludens-1 expression.
36136578	8	39	theme	p	1468:1468	arg1	&lt					1470:1472	p &lt; 0.05	1468:1478	p &lt; 0.05	1468:1478	Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p &lt; 0.05), whereas CIT did not affect zonula occludens-1 expression.
36136578	3	40	theme	innate	651:656	arg1	defense					665:671	innate immune defense	651:671	innate immune defense of the udder	651:684	The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder.
36136578	4	41	theme	barrier	770:776	arg1	functions					796:804	barrier and innate immune functions	770:804	functions	796:804	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	2	42	theme	rumen	347:351	arg1	microflora					353:362	rumen microflora	347:362	rumen microflora following ingestion of contaminated feeds	347:404	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	9	43	theme	IL-6	1673:1676	arg1	Expression					1659:1668	Expression	1659:1668	Expression of IL-6, TNF-a and TGF-β	1659:1693	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	6	44	from	decrease	1037:1044	arg1	flux					1116:1119	paracellular flux	1103:1119	paracellular flux of FITC-40 kDa dextran	1103:1142	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	6	44	from	decrease	1037:1044	arg1	resistance					1076:1085	transepithelial electrical resistance	1049:1085	transepithelial electrical resistance	1049:1085	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	2	45	theme	mycotoxins	333:342	arg1	biodegradation					309:322	Ineffective biodegradation	297:322	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds	297:404	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	8	46	theme	occludens-1	1516:1526	arg1	expression					1528:1537	zonula occludens-1 expression	1509:1537	zonula occludens-1 expression	1509:1537	Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p &lt; 0.05), whereas CIT did not affect zonula occludens-1 expression.
36136578	4	47	theme	epithelium	828:837	arg1	functions					796:804	barrier and innate immune functions	770:804	functions	796:804	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	9	48	theme	TNF-a	1679:1683	arg1	Expression					1659:1668	Expression	1659:1668	Expression of IL-6, TNF-a and TGF-β	1659:1693	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	1	49	theme	potential	233:241	arg1	detection					131:139	Frequent detection	122:139	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff	122:222	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	49	theme	potential	233:241	arg1	threat					243:248	a potential threat	231:248	a potential threat to feed safety, animal performance and health	231:294	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	6	50	theme	kDa	1132:1134	arg1	dextran					1136:1142	FITC-40 kDa dextran	1124:1142	FITC-40 kDa dextran	1124:1142	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	9	51	theme	TGF-β	1689:1693	arg1	Expression					1659:1668	Expression	1659:1668	Expression of IL-6, TNF-a and TGF-β	1659:1693	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	7	52	theme	cytokines	1346:1354	arg1	expression					1275:1284	expression	1275:1284	expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines	1275:1354	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	3	53	theme	mammary	550:556	arg1	epithelium					558:567	The bovine mammary epithelium	539:567	The bovine mammary epithelium	539:567	The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder.
36136578	4	54	theme	bovine	813:818	arg1	epithelium					828:837	the bovine mammary epithelium	809:837	the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T)	809:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	6	55	theme	paracellular	1103:1114	arg1	flux					1116:1119	paracellular flux	1103:1119	paracellular flux of FITC-40 kDa dextran	1103:1142	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	11	56	theme	mycotoxin	2036:2044	arg1	toxicity					2019:2026	the individual toxicity	2004:2026	the individual toxicity of each mycotoxin on bovine mammary epithelium	2004:2073	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	9	57	theme	TLR4	1554:1557	arg1	Expression					1540:1549	Expression	1540:1549	Expression of TLR4	1540:1557	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	9	58	from	h.	1656:1657	arg1	p					1637:1637	p	1637:1637	p &lt; 0.05	1637:1647	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	9	58	from	h.	1656:1657	arg1	CIT					1632:1634	CIT	1632:1634	CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05)	1632:1744	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	5	59	dep	decreased	959:967	arg1	p					1021:1021	p	1021:1021	p &lt; 0.05	1021:1031	Results indicated that OTA and CIT exposure for 48 h significantly decreased cell viability in a concentration-dependent manner (p &lt; 0.05).
36136578	3	60	theme	pivotal	577:583	arg1	role					585:588	a pivotal role	575:588	a pivotal role	575:588	The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder.
36136578	7	61	theme	proteins	1304:1311	arg1	expression					1275:1284	expression	1275:1284	expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines	1275:1354	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	10	62	theme	immune	1921:1926	arg1	functions					1928:1936	barrier and innate immune functions	1902:1936	functions	1928:1936	These results suggest that OTA and CIT could potentially differentially modulate barrier and innate immune functions of mammary epithelium.
36136578	6	63	theme	h	1226:1226	arg1	exposure					1228:1235	48 h exposure	1223:1235	48 h exposure	1223:1235	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	1	64	theme	Frequent	122:129	arg1	detection					131:139	Frequent detection	122:139	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff	122:222	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	64	theme	Frequent	122:129	arg1	threat					243:248	a potential threat	231:248	a potential threat to feed safety, animal performance and health	231:294	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	4	65	theme	mammary	854:860	arg1	MAC-T					884:888	MAC-T	884:888	MAC-T	884:888	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	4	65	theme	mammary	854:860	arg1	line					878:881	a bovine mammary epithelial cell line	845:881	a bovine mammary epithelial cell line (MAC-T)	845:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	6	66	theme	OTA	1173:1175	arg1	treatment					1177:1185	OTA treatment	1173:1185	OTA treatment (p &lt; 0.05)	1173:1199	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	6	66	theme	OTA	1173:1175	arg1	p					1188:1188	p	1188:1188	p &lt; 0.05	1188:1198	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	3	67	theme	milk	605:608	arg1	yield					610:614	milk yield	605:614	milk yield	605:614	The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder.
36136578	4	68	theme	cell	873:876	arg1	MAC-T					884:888	MAC-T	884:888	MAC-T	884:888	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	4	68	theme	cell	873:876	arg1	line					878:881	a bovine mammary epithelial cell line	845:881	a bovine mammary epithelial cell line (MAC-T)	845:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	10	69	theme	epithelium	1949:1958	arg1	functions					1928:1936	barrier and innate immune functions	1902:1936	functions	1928:1936	These results suggest that OTA and CIT could potentially differentially modulate barrier and innate immune functions of mammary epithelium.
36136578	0	70	theme	Cell	76:79	arg1	Permeability					81:92	Bovine Mammary Epithelial Cell Permeability	50:92	Bovine Mammary Epithelial Cell Permeability	50:92	Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.
36136578	1	71	theme	ochratoxin	155:164	arg1	A					166:166	ochratoxin A	155:166	ochratoxin A (OTA)	155:172	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	71	theme	ochratoxin	155:164	arg1	mycotoxins					144:153	mycotoxins ochratoxin A (OTA) and citrinin (CIT)	144:191	mycotoxins ochratoxin A (OTA) and citrinin (CIT)	144:191	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	71	theme	ochratoxin	155:164	arg1	CIT					188:190	CIT	188:190	CIT	188:190	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	71	theme	ochratoxin	155:164	arg1	OTA					169:171	OTA	169:171	OTA	169:171	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	9	72	theme	IL-6	1755:1758	arg1	expression					1770:1779	IL-6 and TGF-β expression	1755:1779	IL-6 and TGF-β expression	1755:1779	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	2	73	theme	mammary	465:471	arg1	gland					473:477	mammary gland	465:477	mammary gland	465:477	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	9	74	theme	TGF-β	1764:1768	arg1	expression					1770:1779	IL-6 and TGF-β expression	1755:1779	IL-6 and TGF-β expression	1755:1779	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	0	75	theme	Immune	105:110	arg1	Function					112:119	Innate Immune Function	98:119	Innate Immune Function	98:119	Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.
36136578	7	76	theme	Toll-like	1314:1322	arg1	receptor					1324:1331	Toll-like receptor 4	1314:1333	Toll-like receptor 4 (TLR4)	1314:1340	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	7	76	theme	Toll-like	1314:1322	arg1	TLR4					1336:1339	TLR4	1336:1339	TLR4	1336:1339	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	1	77	dep	mycotoxins	144:153	arg1	citrinin					178:185	citrinin	178:185	citrinin	178:185	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	77	dep	mycotoxins	144:153	arg1	A					166:166	ochratoxin A	155:166	ochratoxin A (OTA)	155:172	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	77	dep	mycotoxins	144:153	arg1	mycotoxins					144:153	mycotoxins ochratoxin A (OTA) and citrinin (CIT)	144:191	mycotoxins ochratoxin A (OTA) and citrinin (CIT)	144:191	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	77	dep	mycotoxins	144:153	arg1	CIT					188:190	CIT	188:190	CIT	188:190	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	77	dep	mycotoxins	144:153	arg1	OTA					169:171	OTA	169:171	OTA	169:171	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	4	78	theme	individual	733:742	arg1	effects					744:750	individual effects	733:750	individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T)	733:889	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	9	79	dep	upregulated	1577:1587	arg1	whereas					1747:1753	whereas	1747:1753	whereas	1747:1753	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	9	79	dep	upregulated	1577:1587	arg1	p					1807:1807	p	1807:1807	p &lt; 0.01	1807:1817	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	9	80	dep	h.	1656:1657	arg1	upregulated					1713:1723	upregulated	1713:1723	was significantly upregulated by OTA (p &lt; 0.05)	1695:1744	Expression of TLR4 was significantly upregulated by OTA (p &lt; 0.001) but downregulated by CIT (p &lt; 0.05) at 48 h. Expression of IL-6, TNF-a and TGF-β was significantly upregulated by OTA (p &lt; 0.05), whereas IL-6 and TGF-β expression was downregulated by CIT (p &lt; 0.01).
36136578	2	81	theme	circulatory	424:434	arg1	transport					436:444	their circulatory transport	418:444	their circulatory transport to tissues such as mammary gland	418:477	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	1	82	theme	feed	253:256	arg1	safety					258:263	feed safety	253:263	feed safety	253:263	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	83	from	detection	131:139	arg1	feedstuff					214:222	feedstuff	214:222	feedstuff	214:222	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	1	83	from	detection	131:139	arg1	feed					205:208	ruminant feed	196:208	ruminant feed	196:208	Frequent detection of mycotoxins ochratoxin A (OTA) and citrinin (CIT) in ruminant feed and feedstuff can be a potential threat to feed safety, animal performance and health.
36136578	8	84	theme	claudin	1444:1450	arg1	occludin					1458:1465	occludin	1458:1465	occludin (p &lt; 0.05)	1458:1479	Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p &lt; 0.05), whereas CIT did not affect zonula occludens-1 expression.
36136578	8	84	theme	claudin	1444:1450	arg1	expression					1430:1439	expression	1430:1439	expression of claudin 3	1430:1452	Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p &lt; 0.05), whereas CIT did not affect zonula occludens-1 expression.
36136578	8	84	theme	claudin	1444:1450	arg1	&lt					1470:1472	p &lt; 0.05	1468:1478	p &lt; 0.05	1468:1478	Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p &lt; 0.05), whereas CIT did not affect zonula occludens-1 expression.
36136578	5	85	theme	cell	969:972	arg1	viability					974:982	cell viability	969:982	cell viability	969:982	Results indicated that OTA and CIT exposure for 48 h significantly decreased cell viability in a concentration-dependent manner (p &lt; 0.05).
36136578	2	86	dep	contaminated	387:398	arg1	feeds					400:404	feeds	400:404	feeds	400:404	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	2	87	theme	contaminated	387:398	arg1	ingestion					374:382	ingestion	374:382	ingestion of contaminated feeds	374:404	Ineffective biodegradation of these mycotoxins by rumen microflora following ingestion of contaminated feeds can lead to their circulatory transport to tissues such as mammary gland as the result of their biodistribution throughout the body.
36136578	0	88	theme	Mammary	57:63	arg1	Permeability					81:92	Bovine Mammary Epithelial Cell Permeability	50:92	Bovine Mammary Epithelial Cell Permeability	50:92	Ochratoxin A and Citrinin Differentially Modulate Bovine Mammary Epithelial Cell Permeability and Innate Immune Function.
36136578	3	89	theme	immune	658:663	arg1	defense					665:671	innate immune defense	651:671	innate immune defense of the udder	651:684	The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder.
36136578	4	90	theme	immune	789:794	arg1	functions					796:804	barrier and innate immune functions	770:804	functions	796:804	The present study is the first to investigate individual effects of OTA and CIT on barrier and innate immune functions of the bovine mammary epithelium using a bovine mammary epithelial cell line (MAC-T).
36136578	6	91	theme	electrical	1065:1074	arg1	resistance					1076:1085	transepithelial electrical resistance	1049:1085	transepithelial electrical resistance	1049:1085	A decrease in transepithelial electrical resistance and increase in paracellular flux of FITC-40 kDa dextran was significantly induced by OTA treatment (p &lt; 0.05), but not by CIT after 48 h exposure.
36136578	11	92	theme	mycotoxins	2158:2167	arg1	effects					2139:2145	the combined effects	2126:2145	the combined effects of the two mycotoxins	2126:2167	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	8	93	theme	zonula	1509:1514	arg1	occludens-1					1516:1526	zonula occludens-1	1509:1526	zonula occludens-1 expression	1509:1537	Both OTA and CIT markedly downregulated expression of claudin 3 and occludin (p &lt; 0.05), whereas CIT did not affect zonula occludens-1 expression.
36136578	7	94	theme	exposure	1380:1387	arg1	h					1375:1375	4, 24 and 48 h	1362:1375	4, 24 and 48 h of exposure	1362:1387	qPCR was performed for assessment of expression of tight-junction proteins, Toll-like receptor 4 (TLR4) and cytokines after 4, 24 and 48 h of exposure.
36136578	11	95	theme	mammary	2056:2062	arg1	epithelium					2064:2073	bovine mammary epithelium	2049:2073	bovine mammary epithelium	2049:2073	The present study not only throws light on the individual toxicity of each mycotoxin on bovine mammary epithelium but also lays the foundation for future studies on the combined effects of the two mycotoxins.
36136578	3	96	theme	udder	680:684	arg1	defense					665:671	innate immune defense	651:671	innate immune defense of the udder	651:684	The bovine mammary epithelium plays a pivotal role in maintaining milk yield and composition and contributes to innate immune defense of the udder.
35667450	5	0	theme	tag	733:735	arg1	approach					765:772	tandem mass tag (TMT) labeling quantitative approach	721:772	tandem mass tag (TMT) labeling quantitative approach	721:772	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	7	1	theme	side	1063:1066	arg1	effect					1068:1073	a side effect	1061:1073	a side effect on hUMSCs	1061:1083	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	3	2	from	levels	417:422	arg1	MSCs					409:412	MSCs	409:412	MSCs at levels of proteomics and post-translational protein modifications (PTM)	409:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	3	2	from	levels	417:422	arg1	investigations					377:390	investigations	377:390	investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM)	377:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	4	3	theme	mesenchymal	558:568	arg1	hUMSCs					582:587	hUMSCs	582:587	hUMSCs	582:587	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	4	3	theme	mesenchymal	558:568	arg1	cells					575:579	human umbilical cord mesenchymal stem cells	537:579	human umbilical cord mesenchymal stem cells (hUMSCs)	537:588	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	6	4	theme	fold	881:884	arg1	proteins					871:878	66 proteins	868:878	66 proteins (fold change >1.50) that were differentially expressed in long-term culture	868:954	We identified 66 proteins (fold change >1.50) that were differentially expressed in long-term culture.
35667450	6	4	theme	fold	881:884	arg1	change					886:891	fold change >1.50	881:897	fold change >1.50	881:897	We identified 66 proteins (fold change >1.50) that were differentially expressed in long-term culture.
35667450	7	5	theme	post-translational	1184:1201	arg1	modification					1211:1222	post-translational protein modification	1184:1222	post-translational protein modification	1184:1222	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	4	6	theme	cord	553:556	arg1	hUMSCs					582:587	hUMSCs	582:587	hUMSCs	582:587	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	4	6	theme	cord	553:556	arg1	cells					575:579	human umbilical cord mesenchymal stem cells	537:579	human umbilical cord mesenchymal stem cells (hUMSCs)	537:588	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	8	7	theme	MSCs	1315:1318	arg1	aging					1306:1310	aging	1306:1310	aging of MSCs	1306:1318	Glycosylation analysis indicates that cell N-glycan patterns are associated with aging of MSCs.
35667450	7	8	theme	Gene	957:960	arg1	ontology					962:969	Gene ontology	957:969	Gene ontology (GO)	957:974	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	9	theme	Genomes	1012:1018	arg1	ontology					962:969	Gene ontology	957:969	Gene ontology (GO)	957:974	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	3	10	theme	cell	351:354	arg1	aging					356:360	cell aging	351:360	cell aging	351:360	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	2	11	theme	cellular	273:280	arg1	function					282:289	cellular function	273:289	cellular function	273:289	However, long-term culturing of MSCs will induce aging of MSCs, and thereafter impair cellular function.
35667450	4	12	theme	stem	570:573	arg1	hUMSCs					582:587	hUMSCs	582:587	hUMSCs	582:587	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	4	12	theme	stem	570:573	arg1	cells					575:579	human umbilical cord mesenchymal stem cells	537:579	human umbilical cord mesenchymal stem cells (hUMSCs)	537:588	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	7	13	theme	biological	1130:1139	arg1	lysosome					1160:1167	lysosome	1160:1167	lysosome	1160:1167	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	13	theme	biological	1130:1139	arg1	modification					1211:1222	post-translational protein modification	1184:1222	post-translational protein modification	1184:1222	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	13	theme	biological	1130:1139	arg1	processes					1141:1149	biological processes	1130:1149	biological processes	1130:1149	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	13	theme	biological	1130:1139	arg1	autophagy					1170:1178	autophagy	1170:1178	autophagy	1170:1178	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	1	14	theme	regenerative	164:175	arg1	medicine					177:184	regenerative medicine	164:184	regenerative medicine	164:184	Mesenchymal stem cells (MSCs) are considered to be critical for regenerative medicine.
35667450	5	15	theme	quantitative	752:763	arg1	approach					765:772	tandem mass tag (TMT) labeling quantitative approach	721:772	tandem mass tag (TMT) labeling quantitative approach	721:772	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	9	16	theme	cell	1387:1390	arg1	aging					1392:1396	cell aging	1387:1396	cell aging	1387:1396	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	5	17	theme	TMT	738:740	arg1	approach					765:772	tandem mass tag (TMT) labeling quantitative approach	721:772	tandem mass tag (TMT) labeling quantitative approach	721:772	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	5	18	theme	mass	835:838	arg1	spectrometry					840:851	multistage mass spectrometry	824:851	multistage mass spectrometry	824:851	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	5	19	theme	tandem	721:726	arg1	approach					765:772	tandem mass tag (TMT) labeling quantitative approach	721:772	tandem mass tag (TMT) labeling quantitative approach	721:772	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	1	20	theme	Mesenchymal	100:110	arg1	MSCs					124:127	MSCs	124:127	MSCs	124:127	Mesenchymal stem cells (MSCs) are considered to be critical for regenerative medicine.
35667450	1	20	theme	Mesenchymal	100:110	arg1	cells					117:121	Mesenchymal stem cells	100:121	Mesenchymal stem cells (MSCs)	100:128	Mesenchymal stem cells (MSCs) are considered to be critical for regenerative medicine.
35667450	4	21	theme	7	646:646	arg1	days					654:657	either 7 or 30 days	639:657	days	654:657	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	0	22	theme	Proteomics	0:9	arg1	analysis					48:55	Proteomics and post-translational modifications analysis	0:55	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells	0:91	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	1	23	theme	stem	112:115	arg1	MSCs					124:127	MSCs	124:127	MSCs	124:127	Mesenchymal stem cells (MSCs) are considered to be critical for regenerative medicine.
35667450	1	23	theme	stem	112:115	arg1	cells					117:121	Mesenchymal stem cells	100:121	Mesenchymal stem cells (MSCs)	100:128	Mesenchymal stem cells (MSCs) are considered to be critical for regenerative medicine.
35667450	3	24	theme	MSCs	409:412	arg1	investigations					377:390	investigations	377:390	investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM)	377:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	0	25	theme	modifications	34:46	arg1	analysis					48:55	Proteomics and post-translational modifications analysis	0:55	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells	0:91	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	6	26	theme	long-term	938:946	arg1	culture					948:954	long-term culture	938:954	long-term culture	938:954	We identified 66 proteins (fold change >1.50) that were differentially expressed in long-term culture.
35667450	5	27	theme	Proteins	660:667	arg1	changes					669:675	Proteins changes	660:675	Proteins changes during cell culture	660:695	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	9	28	theme	cellular	1402:1409	arg1	controls					1419:1426	cellular quality controls	1402:1426	cellular quality controls	1402:1426	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	5	29	theme	labeling	743:750	arg1	approach					765:772	tandem mass tag (TMT) labeling quantitative approach	721:772	tandem mass tag (TMT) labeling quantitative approach	721:772	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	8	30	theme	Glycosylation	1225:1237	arg1	analysis					1239:1246	Glycosylation analysis	1225:1246	Glycosylation analysis	1225:1246	Glycosylation analysis indicates that cell N-glycan patterns are associated with aging of MSCs.
35667450	0	31	dep	aging	93:97	arg1	analysis					48:55	Proteomics and post-translational modifications analysis	0:55	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells	0:91	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	4	32	theme	umbilical	543:551	arg1	hUMSCs					582:587	hUMSCs	582:587	hUMSCs	582:587	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	4	32	theme	umbilical	543:551	arg1	cells					575:579	human umbilical cord mesenchymal stem cells	537:579	human umbilical cord mesenchymal stem cells (hUMSCs)	537:588	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	7	33	dep	ontology	962:969	arg1	GO					972:973	GO	972:973	GO	972:973	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	3	34	theme	post-translational	442:459	arg1	PTM					484:486	PTM	484:486	PTM	484:486	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	3	34	theme	post-translational	442:459	arg1	modifications					469:481	post-translational protein modifications	442:481	post-translational protein modifications (PTM)	442:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	3	35	theme	cell	395:398	arg1	aging					400:404	cell aging	395:404	cell aging	395:404	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	4	36	theme	human	537:541	arg1	hUMSCs					582:587	hUMSCs	582:587	hUMSCs	582:587	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	4	36	theme	human	537:541	arg1	cells					575:579	human umbilical cord mesenchymal stem cells	537:579	human umbilical cord mesenchymal stem cells (hUMSCs)	537:588	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	3	37	dep	reported	324:331	arg1	important					504:512	important	504:512	important	504:512	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	7	38	theme	protein	1203:1209	arg1	modification					1211:1222	post-translational protein modification	1184:1222	post-translational protein modification	1184:1222	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	9	39	theme	related	1428:1434	arg1	aging					1392:1396	cell aging	1387:1396	cell aging	1387:1396	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	4	40	theme	culture	616:622	arg1	conditions					624:633	different culture conditions	606:633	different culture conditions	606:633	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	5	41	theme	multistage	824:833	arg1	spectrometry					840:851	multistage mass spectrometry	824:851	multistage mass spectrometry	824:851	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	5	42	theme	cell	684:687	arg1	culture					689:695	cell culture	684:695	cell culture	684:695	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	9	43	theme	regenerative	1447:1458	arg1	medicine					1460:1467	regenerative medicine	1447:1467	regenerative medicine	1447:1467	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	7	44	theme	various	1098:1104	arg1	lysosome					1160:1167	lysosome	1160:1167	lysosome	1160:1167	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	44	theme	various	1098:1104	arg1	modification					1211:1222	post-translational protein modification	1184:1222	post-translational protein modification	1184:1222	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	44	theme	various	1098:1104	arg1	functions					1116:1124	various molecular functions	1098:1124	various molecular functions	1098:1124	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	44	theme	various	1098:1104	arg1	autophagy					1170:1178	autophagy	1170:1178	autophagy	1170:1178	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	3	45	theme	proteomics	427:436	arg1	levels					417:422	levels	417:422	levels of proteomics and post-translational protein modifications (PTM)	417:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	0	46	theme	mesenchymal	70:80	arg1	cells					87:91	umbilical mesenchymal stem cells	60:91	umbilical mesenchymal stem cells	60:91	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	9	47	theme	quality	1411:1417	arg1	controls					1419:1426	cellular quality controls	1402:1426	cellular quality controls	1402:1426	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	7	48	theme	Genes	1002:1006	arg1	ontology					962:969	Gene ontology	957:969	Gene ontology (GO)	957:974	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	5	49	theme	mass	728:731	arg1	approach					765:772	tandem mass tag (TMT) labeling quantitative approach	721:772	tandem mass tag (TMT) labeling quantitative approach	721:772	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	8	50	theme	cell	1263:1266	arg1	patterns					1277:1284	cell N-glycan patterns	1263:1284	cell N-glycan patterns	1263:1284	Glycosylation analysis indicates that cell N-glycan patterns are associated with aging of MSCs.
35667450	0	51	theme	umbilical	60:68	arg1	cells					87:91	umbilical mesenchymal stem cells	60:91	umbilical mesenchymal stem cells	60:91	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	4	52	theme	present	522:528	arg1	study					530:534	the present study	518:534	the present study	518:534	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	9	53	from	studies	1376:1382	arg1	controls					1419:1426	cellular quality controls	1402:1426	cellular quality controls	1402:1426	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	9	53	from	studies	1376:1382	arg1	aging					1392:1396	cell aging	1387:1396	cell aging	1387:1396	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	2	54	theme	MSCs	219:222	arg1	culturing					206:214	long-term culturing	196:214	long-term culturing of MSCs	196:222	However, long-term culturing of MSCs will induce aging of MSCs, and thereafter impair cellular function.
35667450	7	55	from	effect	1068:1073	arg1	hUMSCs					1078:1083	hUMSCs	1078:1083	hUMSCs	1078:1083	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	56	theme	Encyclopedia	986:997	arg1	analysis					1027:1034	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis	957:1034	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis	957:1034	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	3	57	theme	protein	461:467	arg1	PTM					484:486	PTM	484:486	PTM	484:486	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	3	57	theme	protein	461:467	arg1	modifications					469:481	post-translational protein modifications	442:481	post-translational protein modifications (PTM)	442:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	2	58	theme	long-term	196:204	arg1	culturing					206:214	long-term culturing	196:214	long-term culturing of MSCs	196:222	However, long-term culturing of MSCs will induce aging of MSCs, and thereafter impair cellular function.
35667450	5	59	theme	N-glycosylation	779:793	arg1	patterns					795:802	N-glycosylation patterns	779:802	N-glycosylation patterns	779:802	Proteins changes during cell culture were investigated using tandem mass tag (TMT) labeling quantitative approach, and N-glycosylation patterns were analyzed using multistage mass spectrometry.
35667450	7	60	theme	ontology	962:969	arg1	analysis					1027:1034	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis	957:1034	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis	957:1034	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	3	61	theme	modifications	469:481	arg1	levels					417:422	levels	417:422	levels of proteomics and post-translational protein modifications (PTM)	417:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	3	62	from	Changes	292:298	arg1	proteomics					303:312	proteomics	303:312	proteomics	303:312	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	0	63	theme	cells	87:91	arg1	analysis					48:55	Proteomics and post-translational modifications analysis	0:55	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells	0:91	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	4	64	theme	different	606:614	arg1	conditions					624:633	different culture conditions	606:633	different culture conditions	606:633	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	2	65	theme	MSCs	245:248	arg1	aging					236:240	aging	236:240	aging of MSCs	236:248	However, long-term culturing of MSCs will induce aging of MSCs, and thereafter impair cellular function.
35667450	4	66	theme	30	651:652	arg1	days					654:657	either 7 or 30 days	639:657	days	654:657	In the present study, human umbilical cord mesenchymal stem cells (hUMSCs) were exposed to different culture conditions for either 7 or 30 days.
35667450	8	67	theme	N-glycan	1268:1275	arg1	patterns					1277:1284	cell N-glycan patterns	1263:1284	cell N-glycan patterns	1263:1284	Glycosylation analysis indicates that cell N-glycan patterns are associated with aging of MSCs.
35667450	9	68	theme	future	1369:1374	arg1	studies					1376:1382	future studies	1369:1382	future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine	1369:1467	Our results presented here should contribute to future studies on cell aging and cellular quality controls related to MSCs as regenerative medicine.
35667450	0	69	theme	stem	82:85	arg1	cells					87:91	umbilical mesenchymal stem cells	60:91	umbilical mesenchymal stem cells	60:91	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	3	70	from	investigations	377:390	arg1	levels					417:422	levels	417:422	levels of proteomics and post-translational protein modifications (PTM)	417:487	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	3	70	from	investigations	377:390	arg1	aging					400:404	cell aging	395:404	cell aging	395:404	Changes in proteomics have been reported to be involved in cell aging, and therefore investigations on cell aging of MSCs at levels of proteomics and post-translational protein modifications (PTM) are ultimately important.
35667450	0	71	theme	post-translational	15:32	arg1	modifications					34:46	post-translational modifications	15:46	post-translational modifications	15:46	Proteomics and post-translational modifications analysis of umbilical mesenchymal stem cells aging.
35667450	7	72	theme	molecular	1106:1114	arg1	lysosome					1160:1167	lysosome	1160:1167	lysosome	1160:1167	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	72	theme	molecular	1106:1114	arg1	modification					1211:1222	post-translational protein modification	1184:1222	post-translational protein modification	1184:1222	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	72	theme	molecular	1106:1114	arg1	functions					1116:1124	various molecular functions	1098:1124	various molecular functions	1098:1124	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35667450	7	72	theme	molecular	1106:1114	arg1	autophagy					1170:1178	autophagy	1170:1178	autophagy	1170:1178	Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that aging exerts a side effect on hUMSCs by affecting various molecular functions and biological processes, such as lysosome, autophagy and post-translational protein modification.
35137655	0	0	theme	curcumin	61:68	arg1	nanoparticles					70:82	silver@curcumin nanoparticles	54:82	silver@curcumin nanoparticles	54:82	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	2	1	contain	possess	331:337	arg2	properties					339:348	properties	339:348	properties of dual antibacterial and tissue repair	339:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	2	1	contain	possess	331:337	arg1	materials					316:324	materials	316:324	materials that possess properties of dual antibacterial and tissue repair	316:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	4	2	theme	Gram-positive	754:766	arg1	bacteria					768:775	both Gram-negative and Gram-positive bacteria	731:775	both Gram-negative and Gram-positive bacteria	731:775	The developed functionalized silver nanoparticles showed effective activity against both Gram-negative and Gram-positive bacteria.
35137655	7	3	theme	β-CD/AgNPs	1036:1045	arg1	chitosan					1047:1054	Cur@β-CD/AgNPs chitosan	1032:1054	Cur@β-CD/AgNPs chitosan	1032:1054	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	2	4	theme	antibacterial	358:370	arg1	properties					339:348	properties	339:348	properties of dual antibacterial and tissue repair	339:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	5	5	theme	β-CD/AgNPs	791:800	arg1	dressing					811:818	Cur@β-CD/AgNPs chitosan dressing	787:818	Cur@β-CD/AgNPs chitosan dressing	787:818	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	0	6	theme	@	60:60	arg1	nanoparticles					70:82	silver@curcumin nanoparticles	54:82	silver@curcumin nanoparticles	54:82	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	1	7	theme	various	149:155	arg1	wounds					162:167	various skin wounds	149:167	various skin wounds	149:167	Healing of various skin wounds is a lengthy process and often combined with bacterial infection and scar formation.
35137655	1	8	theme	scar	238:241	arg1	formation					243:251	scar formation	238:251	scar formation	238:251	Healing of various skin wounds is a lengthy process and often combined with bacterial infection and scar formation.
35137655	6	9	theme	collagen	968:975	arg1	distribution					977:988	the most uniform collagen distribution	951:988	the most uniform collagen distribution	951:988	Moreover, Cur@β-CD/AgNPs chitosan dressing contributed to the most uniform collagen distribution by Masson's trichrome staining.
35137655	5	10	theme	@	790:790	arg1	dressing					811:818	Cur@β-CD/AgNPs chitosan dressing	787:818	Cur@β-CD/AgNPs chitosan dressing	787:818	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	3	11	theme	β-CD/AgNPs	518:527	arg1	nanoparticles					529:541	Cur@β-CD/AgNPs nanoparticles	514:541	Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	514:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	2	12	theme	wound	289:293	arg1	dressing					295:302	Biomimetic electrospun nanofibrous wound dressing	254:302	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair	254:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	0	13	theme	nanoparticles	70:82	arg1	combination					39:49	the combination	35:49	the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing	35:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	0	14	from	combination	39:49	arg1	healing					129:135	wound healing	123:135	wound healing	123:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	4	15	theme	Gram-negative	736:748	arg1	bacteria					768:775	both Gram-negative and Gram-positive bacteria	731:775	both Gram-negative and Gram-positive bacteria	731:775	The developed functionalized silver nanoparticles showed effective activity against both Gram-negative and Gram-positive bacteria.
35137655	2	16	theme	nanofibrous	277:287	arg1	dressing					295:302	Biomimetic electrospun nanofibrous wound dressing	254:302	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair	254:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	0	17	theme	chitosan	100:107	arg1	nanofibers					109:118	electrospun chitosan nanofibers	88:118	electrospun chitosan nanofibers on wound healing	88:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	4	18	theme	developed	651:659	arg1	nanoparticles					683:695	The developed functionalized silver nanoparticles	647:695	The developed functionalized silver nanoparticles	647:695	The developed functionalized silver nanoparticles showed effective activity against both Gram-negative and Gram-positive bacteria.
35137655	2	19	theme	electrospun	265:275	arg1	dressing					295:302	Biomimetic electrospun nanofibrous wound dressing	254:302	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair	254:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	1	20	theme	skin	157:160	arg1	wounds					162:167	various skin wounds	149:167	various skin wounds	149:167	Healing of various skin wounds is a lengthy process and often combined with bacterial infection and scar formation.
35137655	0	21	theme	electrospun	88:98	arg1	nanofibers					109:118	electrospun chitosan nanofibers	88:118	electrospun chitosan nanofibers on wound healing	88:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	3	22	contain	containing	503:512	arg1	material					494:501	a composite chitosan electrospun nanofibrous material	449:501	a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	449:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	3	22	contain	containing	503:512	arg2	nanoparticles					529:541	Cur@β-CD/AgNPs nanoparticles	514:541	Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	514:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	5	23	theme	wound	839:843	arg1	closure					845:851	enhanced wound closure	830:851	enhanced wound closure rates	830:857	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	2	24	theme	Biomimetic	254:263	arg1	dressing					295:302	Biomimetic electrospun nanofibrous wound dressing	254:302	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair	254:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	1	25	theme	wounds	162:167	arg1	Healing					138:144	Healing	138:144	Healing of various skin wounds	138:167	Healing of various skin wounds is a lengthy process and often combined with bacterial infection and scar formation.
35137655	5	26	theme	enhanced	830:837	arg1	closure					845:851	enhanced wound closure	830:851	enhanced wound closure rates	830:857	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	5	27	theme	chitosan	802:809	arg1	dressing					811:818	Cur@β-CD/AgNPs chitosan dressing	787:818	Cur@β-CD/AgNPs chitosan dressing	787:818	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	3	28	theme	synergic	585:592	arg1	effects					594:600	synergic effects	585:600	synergic effects on antibacterial activity and wound healing	585:644	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	5	29	theme	commercial	871:880	arg1	AquacelAg					882:890	commercial AquacelAg	871:890	commercial AquacelAg	871:890	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	3	30	contain	possessed	575:583	arg1	material					494:501	a composite chitosan electrospun nanofibrous material	449:501	a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	449:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	3	30	contain	possessed	575:583	arg2	effects					594:600	synergic effects	585:600	synergic effects on antibacterial activity and wound healing	585:644	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	2	31	theme	repair	383:388	arg1	properties					339:348	properties	339:348	properties of dual antibacterial and tissue repair	339:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	0	32	theme	dressing	17:24	arg1	Effects					0:6	Effects	0:6	Effects of wound dressing	0:24	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	7	33	with	dressing	1093:1100	arg1	properties					1138:1147	antibacterial and antiscarring properties	1107:1147	antibacterial and antiscarring properties	1107:1147	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	1	34	theme	lengthy	174:180	arg1	process					182:188	a lengthy process	172:188	a lengthy process	172:188	Healing of various skin wounds is a lengthy process and often combined with bacterial infection and scar formation.
35137655	0	35	theme	wound	11:15	arg1	dressing					17:24	wound dressing	11:24	wound dressing	11:24	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	0	36	theme	nanofibers	109:118	arg1	combination					39:49	the combination	35:49	the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing	35:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	2	37	theme	dual	353:356	arg1	antibacterial					358:370	dual antibacterial	353:370	dual antibacterial	353:370	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	3	38	theme	chitosan	461:468	arg1	material					494:501	a composite chitosan electrospun nanofibrous material	449:501	a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	449:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	3	39	theme	Cur	514:516	arg1	nanoparticles					529:541	Cur@β-CD/AgNPs nanoparticles	514:541	Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	514:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	4	40	theme	effective	704:712	arg1	activity					714:721	effective activity	704:721	effective activity against both Gram-negative and Gram-positive bacteria	704:775	The developed functionalized silver nanoparticles showed effective activity against both Gram-negative and Gram-positive bacteria.
35137655	6	41	theme	uniform	960:966	arg1	distribution					977:988	the most uniform collagen distribution	951:988	the most uniform collagen distribution	951:988	Moreover, Cur@β-CD/AgNPs chitosan dressing contributed to the most uniform collagen distribution by Masson's trichrome staining.
35137655	0	42	theme	wound	123:127	arg1	healing					129:135	wound healing	123:135	wound healing	123:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	5	43	theme	Cur	787:789	arg1	dressing					811:818	Cur@β-CD/AgNPs chitosan dressing	787:818	Cur@β-CD/AgNPs chitosan dressing	787:818	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	4	44	theme	silver	676:681	arg1	nanoparticles					683:695	The developed functionalized silver nanoparticles	647:695	The developed functionalized silver nanoparticles	647:695	The developed functionalized silver nanoparticles showed effective activity against both Gram-negative and Gram-positive bacteria.
35137655	6	45	theme	β-CD/AgNPs	907:916	arg1	dressing					927:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Moreover, Cur@β-CD/AgNPs chitosan dressing contributed to the most uniform collagen distribution by Masson's trichrome staining.
35137655	7	46	theme	potential	1077:1085	arg1	dressing					1093:1100	a potential wound dressing	1075:1100	a potential wound dressing with antibacterial and antiscarring properties	1075:1147	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	7	47	theme	antiscarring	1125:1136	arg1	properties					1138:1147	antibacterial and antiscarring properties	1107:1147	antibacterial and antiscarring properties	1107:1147	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	6	48	theme	trichrome	1002:1010	arg1	staining					1012:1019	Masson's trichrome staining	993:1019	Masson's trichrome staining	993:1019	Moreover, Cur@β-CD/AgNPs chitosan dressing contributed to the most uniform collagen distribution by Masson's trichrome staining.
35137655	7	49	theme	antibacterial	1107:1119	arg1	properties					1138:1147	antibacterial and antiscarring properties	1107:1147	antibacterial and antiscarring properties	1107:1147	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	6	50	theme	@	906:906	arg1	dressing					927:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Moreover, Cur@β-CD/AgNPs chitosan dressing contributed to the most uniform collagen distribution by Masson's trichrome staining.
35137655	3	51	theme	antibacterial	605:617	arg1	activity					619:626	antibacterial activity	605:626	antibacterial activity	605:626	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	3	52	theme	@	517:517	arg1	nanoparticles					529:541	Cur@β-CD/AgNPs nanoparticles	514:541	Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	514:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	6	53	theme	chitosan	918:925	arg1	dressing					927:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Moreover, Cur@β-CD/AgNPs chitosan dressing contributed to the most uniform collagen distribution by Masson's trichrome staining.
35137655	7	54	theme	wound	1087:1091	arg1	dressing					1093:1100	a potential wound dressing	1075:1100	a potential wound dressing with antibacterial and antiscarring properties	1075:1147	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	6	55	theme	Cur	903:905	arg1	dressing					927:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Cur@β-CD/AgNPs chitosan dressing	903:934	Moreover, Cur@β-CD/AgNPs chitosan dressing contributed to the most uniform collagen distribution by Masson's trichrome staining.
35137655	5	56	theme	closure	845:851	arg1	rates					853:857	enhanced wound closure rates	830:857	enhanced wound closure rates	830:857	In vivo, Cur@β-CD/AgNPs chitosan dressing displayed enhanced wound closure rates compared to commercial AquacelAg.
35137655	7	57	theme	Cur	1032:1034	arg1	chitosan					1047:1054	Cur@β-CD/AgNPs chitosan	1032:1054	Cur@β-CD/AgNPs chitosan	1032:1054	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	3	58	theme	electrospun	470:480	arg1	material					494:501	a composite chitosan electrospun nanofibrous material	449:501	a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	449:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	0	59	from	healing	129:135	arg1	combination					39:49	the combination	35:49	the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing	35:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	3	60	theme	nanofibrous	482:492	arg1	material					494:501	a composite chitosan electrospun nanofibrous material	449:501	a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	449:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	0	61	theme	silver	54:59	arg1	nanoparticles					70:82	silver@curcumin nanoparticles	54:82	silver@curcumin nanoparticles	54:82	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	0	62	from	nanoparticles	70:82	arg1	healing					129:135	wound healing	123:135	wound healing	123:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
35137655	3	63	theme	wound	632:636	arg1	healing					638:644	wound healing	632:644	wound healing	632:644	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	3	64	theme	composite	451:459	arg1	material					494:501	a composite chitosan electrospun nanofibrous material	449:501	a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin	449:573	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	2	65	theme	tissue	376:381	arg1	repair					383:388	tissue repair	376:388	tissue repair	376:388	Biomimetic electrospun nanofibrous wound dressing loaded with materials that possess properties of dual antibacterial and tissue repair would be developed to address this problem.
35137655	3	66	from	effects	594:600	arg1	activity					619:626	antibacterial activity	605:626	antibacterial activity	605:626	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	3	66	from	effects	594:600	arg1	healing					638:644	wound healing	632:644	wound healing	632:644	In this study, a composite chitosan electrospun nanofibrous material containing Cur@β-CD/AgNPs nanoparticles composed of silver and curcumin possessed synergic effects on antibacterial activity and wound healing.
35137655	1	67	theme	bacterial	214:222	arg1	infection					224:232	bacterial infection	214:232	bacterial infection	214:232	Healing of various skin wounds is a lengthy process and often combined with bacterial infection and scar formation.
35137655	4	68	theme	functionalized	661:674	arg1	nanoparticles					683:695	The developed functionalized silver nanoparticles	647:695	The developed functionalized silver nanoparticles	647:695	The developed functionalized silver nanoparticles showed effective activity against both Gram-negative and Gram-positive bacteria.
35137655	7	69	theme	@	1035:1035	arg1	chitosan					1047:1054	Cur@β-CD/AgNPs chitosan	1032:1054	Cur@β-CD/AgNPs chitosan	1032:1054	In brief, Cur@β-CD/AgNPs chitosan nanofibers work as a potential wound dressing with antibacterial and antiscarring properties.
35137655	0	70	from	nanofibers	109:118	arg1	healing					129:135	wound healing	123:135	wound healing	123:135	Effects of wound dressing based on the combination of silver@curcumin nanoparticles and electrospun chitosan nanofibers on wound healing.
36990412	0	0	theme	binding	101:107	arg1	determination					18:30	Primary structure determination	0:30	Primary structure determination	0:30	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	0	theme	binding	101:107	arg1	glycoprotein					109:120	a phosphatidylcholine binding glycoprotein	79:120	a phosphatidylcholine binding glycoprotein of donkey seminal plasma	79:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	2	1	theme	FnII	452:455	arg1	protein					457:463	another FnII protein	444:463	another FnII protein of donkey seminal plasma	444:488	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	2	1	theme	FnII	452:455	arg1	DSP-3					437:441	DSP-3	437:441	DSP-3	437:441	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	1	2	theme	seminal	170:176	arg1	plasma					178:183	the seminal plasma	166:183	the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	166:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	0	3	theme	phosphatidylcholine	81:99	arg1	determination					18:30	Primary structure determination	0:30	Primary structure determination	0:30	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	3	theme	phosphatidylcholine	81:99	arg1	glycoprotein					109:120	a phosphatidylcholine binding glycoprotein	79:120	a phosphatidylcholine binding glycoprotein of donkey seminal plasma	79:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	8	4	theme	plasma	1512:1517	arg1	membrane					1519:1526	sperm plasma membrane	1506:1526	sperm plasma membrane	1506:1526	Binding of DSP-3 to erythrocytes leads to membrane perturbation, suggesting that its binding to sperm plasma membrane could be physiologically significant.
36990412	1	5	theme	plasma	178:183	arg1	proteins					154:161	Major proteins	148:161	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	148:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	5	theme	plasma	178:183	arg1	proteins					344:351	FnII family proteins	332:351	FnII family proteins	332:351	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	5	6	theme	head	968:971	arg1	moiety					979:984	the head group moiety	964:984	the head group moiety of choline phospholipids	964:1009	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	1	7	from	plasma	178:183	arg1	variety					190:196	a variety	188:196	a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	188:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	7	from	plasma	178:183	arg1	bovine					217:222	bovine PDC-109	217:230	bovine PDC-109	217:230	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	7	from	plasma	178:183	arg1	HSP-1/2					240:246	equine HSP-1/2	233:246	equine HSP-1/2	233:246	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	7	from	plasma	178:183	arg1	mammals					201:207	mammals	201:207	mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	201:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	7	from	plasma	178:183	arg1	DSP-1					260:264	donkey DSP-1	253:264	donkey DSP-1	253:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	8	theme	donkey	253:258	arg1	DSP-1					260:264	donkey DSP-1	253:264	donkey DSP-1	253:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	2	9	theme	plasma	483:488	arg1	protein					457:463	another FnII protein	444:463	another FnII protein of donkey seminal plasma	444:488	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	2	9	theme	plasma	483:488	arg1	DSP-3					437:441	DSP-3	437:441	DSP-3	437:441	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	1	10	theme	FnII	332:335	arg1	proteins					154:161	Major proteins	148:161	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	148:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	10	theme	FnII	332:335	arg1	proteins					344:351	FnII family proteins	332:351	FnII family proteins	332:351	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	6	11	theme	oligomers	1148:1156	arg1	mixtures					1123:1130	mixtures	1123:1130	mixtures of polydisperse oligomers	1123:1156	Analysis of DSC data suggested that unlike PDC-109 and DSP-1, which exist as mixtures of polydisperse oligomers, DSP-3 most likely exists as a monomer.
36990412	7	12	theme	binding	1205:1211	arg1	studies					1213:1219	Ligand binding studies	1198:1219	Ligand binding studies monitoring changes in protein intrinsic fluorescence	1198:1272	Ligand binding studies monitoring changes in protein intrinsic fluorescence indicated that DSP-3 binds lyso-phosphatidylcholine (Ka = 1.08 × 105 M-1) with ~80-fold higher affinity than PrC (Ka = 1.39 × 103 M-1).
36990412	4	13	theme	higher	710:715	arg1	homology					717:724	significantly higher homology	696:724	significantly higher homology to HSP-1 (104 identical residues)	696:758	Interestingly, DSP-3 exhibits significantly higher homology to HSP-1 (104 identical residues) than DSP-1 (72 identical residues).
36990412	0	14	theme	seminal	132:138	arg1	plasma					140:145	donkey seminal plasma	125:145	donkey seminal plasma	125:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	1	15	theme	family	337:342	arg1	proteins					154:161	Major proteins	148:161	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	148:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	15	theme	family	337:342	arg1	proteins					344:351	FnII family proteins	332:351	FnII family proteins	332:351	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	4	16	theme	identical	740:748	arg1	residues					750:757	104 identical residues	736:757	104 identical residues	736:757	Interestingly, DSP-3 exhibits significantly higher homology to HSP-1 (104 identical residues) than DSP-1 (72 identical residues).
36990412	4	16	theme	identical	740:748	arg1	HSP-1					729:733	HSP-1	729:733	HSP-1 (104 identical residues)	729:758	Interestingly, DSP-3 exhibits significantly higher homology to HSP-1 (104 identical residues) than DSP-1 (72 identical residues).
36990412	3	17	theme	High-resolution	491:505	arg1	studies					526:532	High-resolution mass-spectrometric studies	491:532	High-resolution mass-spectrometric studies	491:532	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	5	18	theme	scanning	851:858	arg1	calorimetric					860:871	differential scanning calorimetric	838:871	differential scanning calorimetric (DSC)	838:877	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	5	18	theme	scanning	851:858	arg1	DSC					874:876	DSC	874:876	DSC	874:876	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	0	19	theme	donkey	125:130	arg1	plasma					140:145	donkey seminal plasma	125:145	donkey seminal plasma	125:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	5	20	theme	Circular	796:803	arg1	dichroism					805:813	Circular dichroism	796:813	Circular dichroism (CD) spectroscopic	796:832	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	5	20	theme	Circular	796:803	arg1	CD					816:817	CD	816:817	CD	816:817	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	7	21	from	changes	1232:1238	arg1	fluorescence					1261:1272	protein intrinsic fluorescence	1243:1272	protein intrinsic fluorescence	1243:1272	Ligand binding studies monitoring changes in protein intrinsic fluorescence indicated that DSP-3 binds lyso-phosphatidylcholine (Ka = 1.08 × 105 M-1) with ~80-fold higher affinity than PrC (Ka = 1.39 × 103 M-1).
36990412	1	22	contain	contain	266:272	arg1	proteins					344:351	FnII family proteins	332:351	FnII family proteins	332:351	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	22	contain	contain	266:272	arg1	proteins					154:161	Major proteins	148:161	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	148:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	22	contain	contain	266:272	arg2	domains					301:307	fibronectin type-II (FnII) domains	274:307	fibronectin type-II (FnII) domains	274:307	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	6	23	theme	data	1062:1065	arg1	Analysis					1046:1053	Analysis	1046:1053	Analysis of DSC data	1046:1065	Analysis of DSC data suggested that unlike PDC-109 and DSP-1, which exist as mixtures of polydisperse oligomers, DSP-3 most likely exists as a monomer.
36990412	2	24	theme	seminal	475:481	arg1	plasma					483:488	donkey seminal plasma	468:488	donkey seminal plasma	468:488	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	0	25	theme	structure	8:16	arg1	determination					18:30	Primary structure determination	0:30	Primary structure determination	0:30	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	25	theme	structure	8:16	arg1	glycoprotein					109:120	a phosphatidylcholine binding glycoprotein	79:120	a phosphatidylcholine binding glycoprotein of donkey seminal plasma	79:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	6	26	theme	DSC	1058:1060	arg1	data					1062:1065	DSC data	1058:1065	DSC data	1058:1065	Analysis of DSC data suggested that unlike PDC-109 and DSP-1, which exist as mixtures of polydisperse oligomers, DSP-3 most likely exists as a monomer.
36990412	1	27	from	proteins	154:161	arg1	variety					190:196	a variety	188:196	a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	188:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	27	from	proteins	154:161	arg1	bovine					217:222	bovine PDC-109	217:230	bovine PDC-109	217:230	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	27	from	proteins	154:161	arg1	HSP-1/2					240:246	equine HSP-1/2	233:246	equine HSP-1/2	233:246	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	27	from	proteins	154:161	arg1	mammals					201:207	mammals	201:207	mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	201:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	27	from	proteins	154:161	arg1	DSP-1					260:264	donkey DSP-1	253:264	donkey DSP-1	253:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	0	28	gly	glycoprotein	109:120	arg1	determination					18:30	Primary structure determination	0:30	Primary structure determination	0:30	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	28	gly	glycoprotein	109:120	arg1	glycoprotein					109:120	a phosphatidylcholine binding glycoprotein	79:120	a phosphatidylcholine binding glycoprotein of donkey seminal plasma	79:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	2	29	from	studies	426:432	arg1	protein					457:463	another FnII protein	444:463	another FnII protein of donkey seminal plasma	444:488	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	2	29	from	studies	426:432	arg1	DSP-3					437:441	DSP-3	437:441	DSP-3	437:441	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	0	30	theme	Primary	0:6	arg1	determination					18:30	Primary structure determination	0:30	Primary structure determination	0:30	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	30	theme	Primary	0:6	arg1	glycoprotein					109:120	a phosphatidylcholine binding glycoprotein	79:120	a phosphatidylcholine binding glycoprotein of donkey seminal plasma	79:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	5	31	theme	calorimetric	860:871	arg1	studies					879:885	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies	796:885	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies	796:885	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	7	32	theme	higher	1362:1367	arg1	affinity					1369:1376	~80-fold higher affinity	1353:1376	~80-fold higher affinity than PrC (Ka = 1.39 × 103 M-1)	1353:1407	Ligand binding studies monitoring changes in protein intrinsic fluorescence indicated that DSP-3 binds lyso-phosphatidylcholine (Ka = 1.08 × 105 M-1) with ~80-fold higher affinity than PrC (Ka = 1.39 × 103 M-1).
36990412	1	33	theme	fibronectin	274:284	arg1	domains					301:307	fibronectin type-II (FnII) domains	274:307	fibronectin type-II (FnII) domains	274:307	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	34	theme	mammals	201:207	arg1	variety					190:196	a variety	188:196	a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	188:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	34	theme	mammals	201:207	arg1	bovine					217:222	bovine PDC-109	217:230	bovine PDC-109	217:230	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	34	theme	mammals	201:207	arg1	HSP-1/2					240:246	equine HSP-1/2	233:246	equine HSP-1/2	233:246	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	34	theme	mammals	201:207	arg1	mammals					201:207	mammals	201:207	mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	201:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	34	theme	mammals	201:207	arg1	DSP-1					260:264	donkey DSP-1	253:264	donkey DSP-1	253:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	35	theme	type-II	286:292	arg1	domains					301:307	fibronectin type-II (FnII) domains	274:307	fibronectin type-II (FnII) domains	274:307	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	0	36	theme	plasma	140:145	arg1	determination					18:30	Primary structure determination	0:30	Primary structure determination	0:30	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	36	theme	plasma	140:145	arg1	glycoprotein					109:120	a phosphatidylcholine binding glycoprotein	79:120	a phosphatidylcholine binding glycoprotein of donkey seminal plasma	79:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	5	37	theme	phosphorylcholine	938:954	arg1	binding					927:933	binding	927:933	binding	927:933	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	5	37	theme	phosphorylcholine	938:954	arg1	~45 °C					916:921	~45 °C	916:921	~45 °C	916:921	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	5	38	theme	phospholipids	997:1009	arg1	moiety					979:984	the head group moiety	964:984	the head group moiety of choline phospholipids	964:1009	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	5	39	theme	spectroscopic	820:832	arg1	studies					879:885	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies	796:885	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies	796:885	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	3	40	contain	contains	554:561	arg1	DSP-3					548:552	DSP-3	548:552	DSP-3	548:552	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	3	40	contain	contains	554:561	arg2	residues					578:585	106 amino acid residues	563:585	106 amino acid residues	563:585	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	0	41	theme	physicochemical	36:50	arg1	characterization					52:67	physicochemical characterization	36:67	physicochemical characterization	36:67	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	1	42	theme	FnII	295:298	arg1	domains					301:307	fibronectin type-II (FnII) domains	274:307	fibronectin type-II (FnII) domains	274:307	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	3	43	theme	multiple	628:635	arg1	acetylations					637:648	multiple acetylations	628:648	multiple acetylations on the glycans	628:663	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	5	44	theme	choline	989:995	arg1	phospholipids					997:1009	choline phospholipids	989:1009	choline phospholipids	989:1009	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	2	45	theme	donkey	468:473	arg1	plasma					483:488	donkey seminal plasma	468:488	donkey seminal plasma	468:488	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	0	46	theme	DSP-3	72:76	arg1	determination					18:30	Primary structure determination	0:30	Primary structure determination	0:30	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	46	theme	DSP-3	72:76	arg1	characterization					52:67	physicochemical characterization	36:67	physicochemical characterization	36:67	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	0	46	theme	DSP-3	72:76	arg1	glycoprotein					109:120	a phosphatidylcholine binding glycoprotein	79:120	a phosphatidylcholine binding glycoprotein of donkey seminal plasma	79:145	Primary structure determination and physicochemical characterization of DSP-3, a phosphatidylcholine binding glycoprotein of donkey seminal plasma.
36990412	8	47	theme	membrane	1452:1459	arg1	perturbation					1461:1472	membrane perturbation	1452:1472	membrane perturbation	1452:1472	Binding of DSP-3 to erythrocytes leads to membrane perturbation, suggesting that its binding to sperm plasma membrane could be physiologically significant.
36990412	3	48	theme	amino	567:571	arg1	residues					578:585	106 amino acid residues	563:585	106 amino acid residues	563:585	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	7	49	theme	protein	1243:1249	arg1	fluorescence					1261:1272	protein intrinsic fluorescence	1243:1272	protein intrinsic fluorescence	1243:1272	Ligand binding studies monitoring changes in protein intrinsic fluorescence indicated that DSP-3 binds lyso-phosphatidylcholine (Ka = 1.08 × 105 M-1) with ~80-fold higher affinity than PrC (Ka = 1.39 × 103 M-1).
36990412	8	50	theme	sperm	1506:1510	arg1	membrane					1519:1526	sperm plasma membrane	1506:1526	sperm plasma membrane	1506:1526	Binding of DSP-3 to erythrocytes leads to membrane perturbation, suggesting that its binding to sperm plasma membrane could be physiologically significant.
36990412	5	51	theme	dichroism	805:813	arg1	spectroscopic					820:832	Circular dichroism (CD) spectroscopic	796:832	Circular dichroism (CD) spectroscopic	796:832	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	3	52	theme	acid	573:576	arg1	residues					578:585	106 amino acid residues	563:585	106 amino acid residues	563:585	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	2	53	from	understanding	369:381	arg1	proteins					392:399	these proteins	386:399	these proteins	386:399	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	7	54	theme	intrinsic	1251:1259	arg1	fluorescence					1261:1272	protein intrinsic fluorescence	1243:1272	protein intrinsic fluorescence	1243:1272	Ligand binding studies monitoring changes in protein intrinsic fluorescence indicated that DSP-3 binds lyso-phosphatidylcholine (Ka = 1.08 × 105 M-1) with ~80-fold higher affinity than PrC (Ka = 1.39 × 103 M-1).
36990412	6	55	theme	polydisperse	1135:1146	arg1	oligomers					1148:1156	polydisperse oligomers	1135:1156	polydisperse oligomers	1135:1156	Analysis of DSC data suggested that unlike PDC-109 and DSP-1, which exist as mixtures of polydisperse oligomers, DSP-3 most likely exists as a monomer.
36990412	8	56	theme	DSP-3	1421:1425	arg1	Binding					1410:1416	Binding	1410:1416	Binding of DSP-3 to erythrocytes	1410:1441	Binding of DSP-3 to erythrocytes leads to membrane perturbation, suggesting that its binding to sperm plasma membrane could be physiologically significant.
36990412	1	57	theme	Major	148:152	arg1	proteins					154:161	Major proteins	148:161	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	148:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	57	theme	Major	148:152	arg1	proteins					344:351	FnII family proteins	332:351	FnII family proteins	332:351	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	3	58	theme	mass-spectrometric	507:524	arg1	studies					526:532	High-resolution mass-spectrometric studies	491:532	High-resolution mass-spectrometric studies	491:532	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	5	59	theme	thermal	1027:1033	arg1	stability					1035:1043	the thermal stability	1023:1043	the thermal stability	1023:1043	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	4	60	theme	identical	775:783	arg1	DSP-1					765:769	DSP-1	765:769	DSP-1 (72 identical residues)	765:793	Interestingly, DSP-3 exhibits significantly higher homology to HSP-1 (104 identical residues) than DSP-1 (72 identical residues).
36990412	4	60	theme	identical	775:783	arg1	residues					785:792	72 identical residues	772:792	72 identical residues	772:792	Interestingly, DSP-3 exhibits significantly higher homology to HSP-1 (104 identical residues) than DSP-1 (72 identical residues).
36990412	1	61	from	variety	190:196	arg1	proteins					154:161	Major proteins	148:161	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1	148:264	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	1	61	from	variety	190:196	arg1	proteins					344:351	FnII family proteins	332:351	FnII family proteins	332:351	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	5	62	theme	differential	838:849	arg1	calorimetric					860:871	differential scanning calorimetric	838:871	differential scanning calorimetric (DSC)	838:877	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	5	62	theme	differential	838:849	arg1	DSC					874:876	DSC	874:876	DSC	874:876	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	5	63	theme	group	973:977	arg1	moiety					979:984	the head group moiety	964:984	the head group moiety of choline phospholipids	964:1009	Circular dichroism (CD) spectroscopic and differential scanning calorimetric (DSC) studies showed that DSP-3 unfolds at ~45 °C and binding of phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases the thermal stability.
36990412	3	64	gly	glycosylated	610:621	arg1	DSP-3					548:552	DSP-3	548:552	DSP-3	548:552	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
36990412	1	65	theme	equine	233:238	arg1	HSP-1/2					240:246	equine HSP-1/2	233:246	equine HSP-1/2	233:246	Major proteins of the seminal plasma in a variety of mammals such as bovine PDC-109, equine HSP-1/2, and donkey DSP-1 contain fibronectin type-II (FnII) domains and are referred to as FnII family proteins.
36990412	2	66	theme	detailed	417:424	arg1	studies					426:432	detailed studies	417:432	detailed studies on DSP-3, another FnII protein of donkey seminal plasma	417:488	To further our understanding on these proteins, we carried out detailed studies on DSP-3, another FnII protein of donkey seminal plasma.
36990412	7	67	theme	Ligand	1198:1203	arg1	studies					1213:1219	Ligand binding studies	1198:1219	Ligand binding studies monitoring changes in protein intrinsic fluorescence	1198:1272	Ligand binding studies monitoring changes in protein intrinsic fluorescence indicated that DSP-3 binds lyso-phosphatidylcholine (Ka = 1.08 × 105 M-1) with ~80-fold higher affinity than PrC (Ka = 1.39 × 103 M-1).
36990412	3	68	from	acetylations	637:648	arg1	glycans					657:663	the glycans	653:663	the glycans	653:663	High-resolution mass-spectrometric studies revealed that DSP-3 contains 106 amino acid residues and is heterogeneously glycosylated with multiple acetylations on the glycans.
35546537	8	0	theme	region	1110:1115	arg1	domain					1117:1122	a conserved periplasmic homology region domain	1077:1122	a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain	1077:1182	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	6	1	dep	D.	864:865	arg1	dadantii					867:874	D. dadantii	864:874	D. dadantii	864:874	Here, we show that in D. dadantii, the pilotin acts in concert with the scaffolding protein GspB.
35546537	11	2	theme	domain	1610:1615	arg1	composition					1617:1627	domain composition	1610:1627	domain composition	1610:1627	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	11	3	theme	Phylogenetic	1525:1536	arg1	analysis					1538:1545	Phylogenetic analysis	1525:1545	Phylogenetic analysis	1525:1545	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	3	4	theme	double	498:503	arg1	envelope					519:526	the double membrane cell envelope	494:526	the double membrane cell envelope	494:526	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	18	5	theme	other	2727:2731	arg1	T2SSs					2733:2737	many other T2SSs	2722:2737	many other T2SSs	2722:2737	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	12	6	theme	cell	1746:1749	arg1	membranes					1751:1759	two cell membranes	1742:1759	two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope	1742:1844	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	0	7	theme	Cell	192:195	arg1	Wall					197:200	the Peptidoglycan Cell Wall	174:200	the Peptidoglycan Cell Wall	174:200	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	9	8	theme	Site-specific	1185:1197	arg1	photo-cross-linking					1199:1217	Site-specific photo-cross-linking	1185:1217	Site-specific photo-cross-linking	1185:1217	Site-specific photo-cross-linking unravels molecular details of the GspB-GspD complex in vivo.
35546537	8	9	theme	secretin	1168:1175	arg1	domain					1177:1182	the N-terminal secretin domain	1153:1182	the N-terminal secretin domain	1153:1182	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	12	10	theme	Gram-negative	1714:1726	arg1	bacteria					1728:1735	IMPORTANCE Gram-negative bacteria	1703:1735	IMPORTANCE Gram-negative bacteria	1703:1735	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	17	11	theme	other	2468:2472	arg1	bacteria					2474:2481	some other bacteria	2463:2481	some other bacteria	2463:2481	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	0	12	theme	Outer	116:120	arg1	Pore					140:143	the Outer Membrane Secretin Pore	112:143	the Outer Membrane Secretin Pore	112:143	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	5	13	theme	secretins	807:815	arg1	targeting					781:789	targeting	781:789	targeting	781:789	Usually, specialized lipoproteins termed pilotins assist the targeting and assembly of secretins into the outer membrane.
35546537	5	13	theme	secretins	807:815	arg1	assembly					795:802	assembly	795:802	assembly	795:802	Usually, specialized lipoproteins termed pilotins assist the targeting and assembly of secretins into the outer membrane.
35546537	18	14	theme	domain	2688:2693	arg1	composition					2695:2705	varied domain composition	2681:2705	varied domain composition	2681:2705	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	0	15	theme	Secretin	131:138	arg1	Pore					140:143	the Outer Membrane Secretin Pore	112:143	the Outer Membrane Secretin Pore	112:143	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	13	16	theme	specialized	1943:1953	arg1	systems					1965:1971	several specialized secretion systems	1935:1971	several specialized secretion systems	1935:1971	To translocate effector proteins across this multilayer envelope, bacteria have evolved several specialized secretion systems.
35546537	17	17	theme	cell	2653:2656	arg1	wall					2658:2661	the bacterial cell wall	2639:2661	the bacterial cell wall	2639:2661	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	4	18	theme	secretion	682:690	arg1	system					692:697	the type 3 secretion system	671:697	the type 3 secretion system	671:697	Secretins are also found in the type 3 secretion system and the type 4 pili.
35546537	7	19	theme	pectinase	995:1003	arg1	secretion					1005:1013	pectinase secretion	995:1013	pectinase secretion	995:1013	Deletion of gspB profoundly impacts secretin assembly, pectinase secretion, and virulence.
35546537	3	20	theme	outer	579:583	arg1	pore					594:597	a large outer membrane pore	571:597	a large outer membrane pore formed by 15 subunits of the secretin GspD	571:640	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	18	21	theme	varied	2681:2686	arg1	composition					2695:2705	varied domain composition	2681:2705	varied domain composition	2681:2705	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	0	22	theme	Inner	152:156	arg1	Membrane					158:165	the Inner Membrane	148:165	the Inner Membrane	148:165	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	9	23	theme	complex	1263:1269	arg1	details					1238:1244	molecular details	1228:1244	molecular details of the GspB-GspD complex	1228:1269	Site-specific photo-cross-linking unravels molecular details of the GspB-GspD complex in vivo.
35546537	18	24	from	present	2711:2717	arg1	T2SSs					2733:2737	many other T2SSs	2722:2737	many other T2SSs	2722:2737	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	18	25	from	T2SSs	2733:2737	arg1	present					2711:2717	present	2711:2717	present	2711:2717	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	16	26	theme	subunits	2319:2326	arg1	Targeting					2297:2305	Targeting	2297:2305	Targeting of secretin subunits into the outer membrane	2297:2350	Targeting of secretin subunits into the outer membrane is often facilitated by a special class of lipoproteins called pilotins.
35546537	0	27	theme	dadantii	66:73	arg1	System					92:97	the Dickeya dadantii Type 2 Secretion System	54:97	the Dickeya dadantii Type 2 Secretion System	54:97	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	15	28	theme	new	2277:2279	arg1	antibacterials					2281:2294	new antibacterials	2277:2294	new antibacterials	2277:2294	The secretins are essential for nutrient acquisition and pathogenicity and constitute a target for development of new antibacterials.
35546537	17	29	theme	inner	2620:2624	arg1	membrane					2626:2633	the inner membrane	2616:2633	the inner membrane	2616:2633	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	4	30	theme	type	675:678	arg1	system					692:697	the type 3 secretion system	671:697	the type 3 secretion system	671:697	Secretins are also found in the type 3 secretion system and the type 4 pili.
35546537	5	31	theme	specialized	729:739	arg1	lipoproteins					741:752	specialized lipoproteins	729:752	specialized lipoproteins termed pilotins	729:768	Usually, specialized lipoproteins termed pilotins assist the targeting and assembly of secretins into the outer membrane.
35546537	1	32	theme	type	347:350	arg1	system					364:369	the type 2 secretion system	343:369	the type 2 secretion system (T2SS)	343:376	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	1	32	theme	type	347:350	arg1	T2SS					372:375	T2SS	372:375	T2SS	372:375	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	15	33	theme	nutrient	2195:2202	arg1	acquisition					2204:2214	nutrient acquisition	2195:2214	nutrient acquisition	2195:2214	The secretins are essential for nutrient acquisition and pathogenicity and constitute a target for development of new antibacterials.
35546537	3	34	theme	secretin	628:635	arg1	GspD					637:640	the secretin GspD	624:640	the secretin GspD	624:640	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	10	35	theme	C-terminal	1419:1428	arg1	extension					1430:1438	the C-terminal extension	1415:1438	the C-terminal extension of GspB	1415:1446	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	13	36	theme	multilayer	1892:1901	arg1	envelope					1903:1910	this multilayer envelope	1887:1910	this multilayer envelope	1887:1910	To translocate effector proteins across this multilayer envelope, bacteria have evolved several specialized secretion systems.
35546537	12	37	contain	have	1737:1740	arg2	membranes					1751:1759	two cell membranes	1742:1759	two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope	1742:1844	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	12	37	contain	have	1737:1740	arg1	bacteria					1728:1735	IMPORTANCE Gram-negative bacteria	1703:1735	IMPORTANCE Gram-negative bacteria	1703:1735	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	0	38	theme	System	92:97	arg1	Assembly					42:49	Assembly	42:49	Assembly of the Dickeya dadantii Type 2 Secretion System	42:97	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	1	39	theme	secretion	354:362	arg1	system					364:369	the type 2 secretion system	343:369	the type 2 secretion system (T2SS)	343:376	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	1	39	theme	secretion	354:362	arg1	T2SS					372:375	T2SS	372:375	T2SS	372:375	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	14	40	theme	other	2014:2018	arg1	machineries					2030:2040	some other bacterial machineries	2009:2040	some other bacterial machineries	2009:2040	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	6	41	theme	protein	926:932	arg1	GspB					934:937	the scaffolding protein GspB	910:937	the scaffolding protein GspB	910:937	Here, we show that in D. dadantii, the pilotin acts in concert with the scaffolding protein GspB.
35546537	14	42	theme	effector	2105:2112	arg1	proteins					2114:2121	effector proteins	2105:2121	effector proteins	2105:2121	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	15	43	theme	antibacterials	2281:2294	arg1	development					2262:2272	development	2262:2272	development of new antibacterials	2262:2294	The secretins are essential for nutrient acquisition and pathogenicity and constitute a target for development of new antibacterials.
35546537	4	44	theme	type	707:710	arg1	pili					714:717	the type 4 pili	703:717	the type 4 pili	703:717	Secretins are also found in the type 3 secretion system and the type 4 pili.
35546537	13	45	theme	effector	1862:1869	arg1	proteins					1871:1878	effector proteins	1862:1878	effector proteins	1862:1878	To translocate effector proteins across this multilayer envelope, bacteria have evolved several specialized secretion systems.
35546537	0	46	theme	Scaffolding	0:10	arg1	GspB/OutB					20:28	Scaffolding Protein GspB/OutB	0:28	Scaffolding Protein GspB/OutB	0:28	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	1	47	theme	plant	277:281	arg1	enzymes					303:309	plant cell wall-degrading enzymes	277:309	plant cell wall-degrading enzymes	277:309	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	14	48	theme	secretion	1988:1996	arg1	system					1998:2003	the type 2 secretion system	1977:2003	the type 2 secretion system	1977:2003	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	17	49	theme	secretin	2576:2583	arg1	pore					2585:2588	the secretin pore	2572:2588	the secretin pore	2572:2588	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	1	50	theme	wall-degrading	288:301	arg1	enzymes					303:309	plant cell wall-degrading enzymes	277:309	plant cell wall-degrading enzymes	277:309	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	14	51	theme	type	1981:1984	arg1	system					1998:2003	the type 2 secretion system	1977:2003	the type 2 secretion system	1977:2003	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	14	52	theme	multimeric	2064:2073	arg1	pores					2075:2079	large multimeric pores	2058:2079	large multimeric pores that allow transport of effector proteins or filaments across the outer membrane	2058:2160	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	3	53	theme	GspD	637:640	arg1	subunits					612:619	15 subunits	609:619	15 subunits of the secretin GspD	609:640	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	2	54	theme	bacterial	441:449	arg1	pathogens					451:459	human bacterial pathogens	435:459	human bacterial pathogens	435:459	T2SSs are widespread among important plant, animal, and human bacterial pathogens.
35546537	10	55	theme	cell	1514:1517	arg1	wall					1519:1522	the peptidoglycan cell wall	1496:1522	the peptidoglycan cell wall	1496:1522	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	11	56	theme	cognate	1662:1668	arg1	GspA					1677:1680	a cognate ATPase GspA	1660:1680	a cognate ATPase GspA	1660:1680	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	8	57	contain	possesses	1067:1075	arg1	GspB					1062:1065	GspB	1062:1065	GspB	1062:1065	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	8	57	contain	possesses	1067:1075	arg2	domain					1117:1122	a conserved periplasmic homology region domain	1077:1122	a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain	1077:1182	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	1	58	theme	virulence	321:329	arg1	factors					331:337	other virulence factors	315:337	other virulence factors	315:337	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	12	59	theme	robust	1814:1819	arg1	envelope					1837:1844	a robust protective cell envelope	1812:1844	a robust protective cell envelope	1812:1844	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	10	60	theme	membrane	1316:1323	arg1	targeting					1325:1333	outer membrane targeting	1310:1333	outer membrane targeting	1310:1333	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	11	61	with	concert	1640:1646	arg1	GspA					1677:1680	a cognate ATPase GspA	1660:1680	a cognate ATPase GspA	1660:1680	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	11	61	with	concert	1640:1646	arg1	GspS					1697:1700	the pilotin GspS	1685:1700	the pilotin GspS	1685:1700	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	1	62	theme	phytopathogenic	207:221	arg1	dadantii					247:254	The phytopathogenic proteobacterium Dickeya dadantii	203:254	The phytopathogenic proteobacterium Dickeya dadantii	203:254	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	12	63	theme	cell	1832:1835	arg1	envelope					1837:1844	a robust protective cell envelope	1812:1844	a robust protective cell envelope	1812:1844	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	17	64	theme	protein	2500:2506	arg1	GspB					2508:2511	the scaffolding protein GspB	2484:2511	the scaffolding protein GspB	2484:2511	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	10	65	theme	secretin	1355:1362	arg1	pores					1364:1368	the secretin pores	1351:1368	the secretin pores	1351:1368	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	3	66	theme	membrane	505:512	arg1	envelope					519:526	the double membrane cell envelope	494:526	the double membrane cell envelope	494:526	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	1	67	theme	Dickeya	239:245	arg1	dadantii					247:254	The phytopathogenic proteobacterium Dickeya dadantii	203:254	The phytopathogenic proteobacterium Dickeya dadantii	203:254	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	8	68	theme	conserved	1079:1087	arg1	domain					1117:1122	a conserved periplasmic homology region domain	1077:1122	a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain	1077:1182	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	12	69	theme	net	1789:1791	arg1	peptidoglycan					1775:1787	a peptidoglycan net	1773:1791	a peptidoglycan net that together form a robust protective cell envelope	1773:1844	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	18	70	located	present	2711:2717	arg1	T2SSs					2733:2737	many other T2SSs	2722:2737	many other T2SSs	2722:2737	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	18	70	located	present	2711:2717	arg2	homologs					2669:2676	GspB homologs	2664:2676	GspB homologs of varied domain composition	2664:2705	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	8	71	theme	homology	1101:1108	arg1	domain					1117:1122	a conserved periplasmic homology region domain	1077:1122	a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain	1077:1182	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	13	72	theme	several	1935:1941	arg1	systems					1965:1971	several specialized secretion systems	1935:1971	several specialized secretion systems	1935:1971	To translocate effector proteins across this multilayer envelope, bacteria have evolved several specialized secretion systems.
35546537	0	73	theme	Peptidoglycan	178:190	arg1	Wall					197:200	the Peptidoglycan Cell Wall	174:200	the Peptidoglycan Cell Wall	174:200	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	5	74	dep	targeting	781:789	arg1	the					777:779	the	777:779	the	777:779	Usually, specialized lipoproteins termed pilotins assist the targeting and assembly of secretins into the outer membrane.
35546537	6	75	with	concert	897:903	arg1	GspB					934:937	the scaffolding protein GspB	910:937	the scaffolding protein GspB	910:937	Here, we show that in D. dadantii, the pilotin acts in concert with the scaffolding protein GspB.
35546537	18	76	theme	many	2722:2725	arg1	T2SSs					2733:2737	many other T2SSs	2722:2737	many other T2SSs	2722:2737	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	5	77	theme	outer	826:830	arg1	membrane					832:839	the outer membrane	822:839	the outer membrane	822:839	Usually, specialized lipoproteins termed pilotins assist the targeting and assembly of secretins into the outer membrane.
35546537	12	78	theme	IMPORTANCE	1703:1712	arg1	bacteria					1728:1735	IMPORTANCE Gram-negative bacteria	1703:1735	IMPORTANCE Gram-negative bacteria	1703:1735	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	11	79	theme	other	1561:1565	arg1	bacteria					1567:1574	other bacteria	1561:1574	other bacteria	1561:1574	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	6	80	theme	scaffolding	914:924	arg1	GspB					934:937	the scaffolding protein GspB	910:937	the scaffolding protein GspB	910:937	Here, we show that in D. dadantii, the pilotin acts in concert with the scaffolding protein GspB.
35546537	10	81	theme	inner	1394:1398	arg1	membrane					1400:1407	the inner membrane	1390:1407	the inner membrane	1390:1407	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	0	82	theme	Membrane	122:129	arg1	Pore					140:143	the Outer Membrane Secretin Pore	112:143	the Outer Membrane Secretin Pore	112:143	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	13	83	theme	secretion	1955:1963	arg1	systems					1965:1971	several specialized secretion systems	1935:1971	several specialized secretion systems	1935:1971	To translocate effector proteins across this multilayer envelope, bacteria have evolved several specialized secretion systems.
35546537	3	84	theme	folded	547:552	arg1	proteins					554:561	fully folded proteins	541:561	fully folded proteins	541:561	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	16	85	theme	lipoproteins	2395:2406	arg1	class					2386:2390	a special class	2376:2390	a special class of lipoproteins called pilotins	2376:2422	Targeting of secretin subunits into the outer membrane is often facilitated by a special class of lipoproteins called pilotins.
35546537	8	86	theme	N-terminal	1157:1166	arg1	domain					1177:1182	the N-terminal secretin domain	1153:1182	the N-terminal secretin domain	1153:1182	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	9	87	theme	molecular	1228:1236	arg1	details					1238:1244	molecular details	1228:1244	molecular details of the GspB-GspD complex	1228:1269	Site-specific photo-cross-linking unravels molecular details of the GspB-GspD complex in vivo.
35546537	3	88	theme	membrane	585:592	arg1	pore					594:597	a large outer membrane pore	571:597	a large outer membrane pore formed by 15 subunits of the secretin GspD	571:640	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	17	89	theme	bacterial	2643:2651	arg1	wall					2658:2661	the bacterial cell wall	2639:2661	the bacterial cell wall	2639:2661	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	17	90	theme	pore	2585:2588	arg1	anchoring					2598:2606	its anchoring	2594:2606	its anchoring to both the inner membrane and the bacterial cell wall	2594:2661	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	17	90	theme	pore	2585:2588	arg1	assembly					2560:2567	the assembly	2556:2567	the assembly of the secretin pore	2556:2588	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	14	91	theme	outer	2147:2151	arg1	membrane					2153:2160	the outer membrane	2143:2160	the outer membrane	2143:2160	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	3	92	theme	large	573:577	arg1	pore					594:597	a large outer membrane pore	571:597	a large outer membrane pore formed by 15 subunits of the secretin GspD	571:640	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	16	93	theme	secretin	2310:2317	arg1	subunits					2319:2326	secretin subunits	2310:2326	secretin subunits	2310:2326	Targeting of secretin subunits into the outer membrane is often facilitated by a special class of lipoproteins called pilotins.
35546537	7	94	theme	secretin	976:983	arg1	assembly					985:992	secretin assembly	976:992	secretin assembly	976:992	Deletion of gspB profoundly impacts secretin assembly, pectinase secretion, and virulence.
35546537	9	95	theme	GspB-GspD	1253:1261	arg1	complex					1263:1269	the GspB-GspD complex	1249:1269	the GspB-GspD complex	1249:1269	Site-specific photo-cross-linking unravels molecular details of the GspB-GspD complex in vivo.
35546537	7	96	theme	gspB	952:955	arg1	Deletion					940:947	Deletion	940:947	Deletion of gspB	940:955	Deletion of gspB profoundly impacts secretin assembly, pectinase secretion, and virulence.
35546537	17	97	dep	D.	2447:2448	arg1	dadantii					2450:2457	D. dadantii	2447:2457	D. dadantii	2447:2457	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	0	98	theme	Type	75:78	arg1	System					92:97	the Dickeya dadantii Type 2 Secretion System	54:97	the Dickeya dadantii Type 2 Secretion System	54:97	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	18	99	theme	composition	2695:2705	arg1	homologs					2669:2676	GspB homologs	2664:2676	GspB homologs of varied domain composition	2664:2705	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	10	100	from	channel	1485:1491	arg1	wall					1519:1522	the peptidoglycan cell wall	1496:1522	the peptidoglycan cell wall	1496:1522	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	16	101	theme	outer	2337:2341	arg1	membrane					2343:2350	the outer membrane	2333:2350	the outer membrane	2333:2350	Targeting of secretin subunits into the outer membrane is often facilitated by a special class of lipoproteins called pilotins.
35546537	0	102	theme	Secretion	82:90	arg1	System					92:97	the Dickeya dadantii Type 2 Secretion System	54:97	the Dickeya dadantii Type 2 Secretion System	54:97	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	18	103	attach	present	2711:2717	arg1	T2SSs					2733:2737	many other T2SSs	2722:2737	many other T2SSs	2722:2737	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	18	103	attach	present	2711:2717	arg2	homologs					2669:2676	GspB homologs	2664:2676	GspB homologs of varied domain composition	2664:2705	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	14	104	theme	filaments	2126:2134	arg1	transport					2092:2100	transport	2092:2100	transport of effector proteins or filaments across the outer membrane	2092:2160	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	14	105	theme	bacterial	2020:2028	arg1	machineries					2030:2040	some other bacterial machineries	2009:2040	some other bacterial machineries	2009:2040	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	4	106	located	found	662:666	arg2	Secretins					643:651	Secretins	643:651	Secretins	643:651	Secretins are also found in the type 3 secretion system and the type 4 pili.
35546537	4	106	located	found	662:666	arg1	pili					714:717	the type 4 pili	703:717	the type 4 pili	703:717	Secretins are also found in the type 3 secretion system and the type 4 pili.
35546537	4	106	located	found	662:666	arg1	system					692:697	the type 3 secretion system	671:697	the type 3 secretion system	671:697	Secretins are also found in the type 3 secretion system and the type 4 pili.
35546537	14	107	theme	proteins	2114:2121	arg1	transport					2092:2100	transport	2092:2100	transport of effector proteins or filaments across the outer membrane	2092:2160	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	18	108	theme	GspB	2664:2667	arg1	homologs					2669:2676	GspB homologs	2664:2676	GspB homologs of varied domain composition	2664:2705	GspB homologs of varied domain composition are present in many other T2SSs.
35546537	0	109	theme	Protein	12:18	arg1	GspB/OutB					20:28	Scaffolding Protein GspB/OutB	0:28	Scaffolding Protein GspB/OutB	0:28	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	10	110	theme	GspB	1443:1446	arg1	extension					1430:1438	the C-terminal extension	1415:1438	the C-terminal extension of GspB	1415:1446	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	16	111	theme	special	2378:2384	arg1	class					2386:2390	a special class	2376:2390	a special class of lipoproteins called pilotins	2376:2422	Targeting of secretin subunits into the outer membrane is often facilitated by a special class of lipoproteins called pilotins.
35546537	10	112	theme	outer	1310:1314	arg1	targeting					1325:1333	outer membrane targeting	1310:1333	outer membrane targeting	1310:1333	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	1	113	theme	cell	283:286	arg1	enzymes					303:309	plant cell wall-degrading enzymes	277:309	plant cell wall-degrading enzymes	277:309	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	8	114	theme	Structural	1031:1040	arg1	studies					1042:1048	Structural studies	1031:1048	Structural studies	1031:1048	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	10	115	theme	secretin	1476:1483	arg1	channel					1485:1491	the secretin channel	1472:1491	the secretin channel in the peptidoglycan cell wall	1472:1522	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	11	116	theme	ATPase	1670:1675	arg1	GspA					1677:1680	a cognate ATPase GspA	1660:1680	a cognate ATPase GspA	1660:1680	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	2	117	theme	important	406:414	arg1	plant					416:420	important plant	406:420	important plant	406:420	T2SSs are widespread among important plant, animal, and human bacterial pathogens.
35546537	1	118	theme	enzymes	303:309	arg1	array					268:272	an array	265:272	an array of plant cell wall-degrading enzymes and other virulence factors	265:337	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	14	119	theme	large	2058:2062	arg1	pores					2075:2079	large multimeric pores	2058:2079	large multimeric pores that allow transport of effector proteins or filaments across the outer membrane	2058:2160	In the type 2 secretion system and some other bacterial machineries, secretins form large multimeric pores that allow transport of effector proteins or filaments across the outer membrane.
35546537	11	120	theme	GspB	1577:1580	arg1	homologs					1582:1589	GspB homologs	1577:1589	GspB homologs	1577:1589	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	0	121	theme	Dickeya	58:64	arg1	System					92:97	the Dickeya dadantii Type 2 Secretion System	54:97	the Dickeya dadantii Type 2 Secretion System	54:97	Scaffolding Protein GspB/OutB Facilitates Assembly of the Dickeya dadantii Type 2 Secretion System by Anchoring the Outer Membrane Secretin Pore to the Inner Membrane and to the Peptidoglycan Cell Wall.
35546537	1	122	theme	other	315:319	arg1	factors					331:337	other virulence factors	315:337	other virulence factors	315:337	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	12	123	theme	protective	1821:1830	arg1	envelope					1837:1844	a robust protective cell envelope	1812:1844	a robust protective cell envelope	1812:1844	IMPORTANCE Gram-negative bacteria have two cell membranes sandwiching a peptidoglycan net that together form a robust protective cell envelope.
35546537	2	124	theme	human	435:439	arg1	pathogens					451:459	human bacterial pathogens	435:459	human bacterial pathogens	435:459	T2SSs are widespread among important plant, animal, and human bacterial pathogens.
35546537	10	125	theme	peptidoglycan	1500:1512	arg1	wall					1519:1522	the peptidoglycan cell wall	1496:1522	the peptidoglycan cell wall	1496:1522	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	11	126	theme	pilotin	1689:1695	arg1	GspS					1697:1700	the pilotin GspS	1685:1700	the pilotin GspS	1685:1700	Phylogenetic analysis shows that in other bacteria, GspB homologs vary in length and domain composition and act in concert with either a cognate ATPase GspA or the pilotin GspS.
35546537	1	127	theme	factors	331:337	arg1	array					268:272	an array	265:272	an array of plant cell wall-degrading enzymes and other virulence factors	265:337	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	17	128	theme	scaffolding	2488:2498	arg1	GspB					2508:2511	the scaffolding protein GspB	2484:2511	the scaffolding protein GspB	2484:2511	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	10	129	theme	pores	1364:1368	arg1	assembly					1339:1346	assembly	1339:1346	assembly	1339:1346	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	10	129	theme	pores	1364:1368	arg1	targeting					1325:1333	outer membrane targeting	1310:1333	outer membrane targeting	1310:1333	We show that GspB facilitates outer membrane targeting and assembly of the secretin pores and anchors them to the inner membrane while the C-terminal extension of GspB provides a scaffold for the secretin channel in the peptidoglycan cell wall.
35546537	3	130	theme	multiprotein	467:478	arg1	complex					480:486	This multiprotein complex	462:486	This multiprotein complex	462:486	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
35546537	1	131	theme	proteobacterium	223:237	arg1	dadantii					247:254	The phytopathogenic proteobacterium Dickeya dadantii	203:254	The phytopathogenic proteobacterium Dickeya dadantii	203:254	The phytopathogenic proteobacterium Dickeya dadantii secretes an array of plant cell wall-degrading enzymes and other virulence factors via the type 2 secretion system (T2SS).
35546537	8	132	theme	periplasmic	1089:1099	arg1	domain					1117:1122	a conserved periplasmic homology region domain	1077:1122	a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain	1077:1182	Structural studies reveal that GspB possesses a conserved periplasmic homology region domain that interacts directly with the N-terminal secretin domain.
35546537	17	133	with	concert	2521:2527	arg1	pilotin					2534:2540	pilotin	2534:2540	pilotin	2534:2540	Here, we show that in D. dadantii and some other bacteria, the scaffolding protein GspB acts in concert with pilotin, facilitating the assembly of the secretin pore and its anchoring to both the inner membrane and the bacterial cell wall.
35546537	3	134	theme	cell	514:517	arg1	envelope					519:526	the double membrane cell envelope	494:526	the double membrane cell envelope	494:526	This multiprotein complex spans the double membrane cell envelope and secretes fully folded proteins through a large outer membrane pore formed by 15 subunits of the secretin GspD.
33763999	8	0	from	clearance	1165:1173	arg1	patients					1185:1192	patients	1185:1192	patients with von Willebrand disease	1185:1220	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	6	1	theme	globular	885:892	arg1	VWF					894:896	globular VWF	885:896	globular VWF	885:896	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	7	2	theme	MGLmediated	1073:1083	arg1	clearance					1085:1093	MGLmediated clearance	1073:1093	MGLmediated clearance	1073:1093	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	12	3	theme	von	1972:1974	arg1	disease					1987:1993	von Willebrand disease	1972:1993	von Willebrand disease	1972:1993	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	0	4	link	O-linked	15:22	arg1	glycans					24:30	O-linked glycans	15:30	O-linked glycans	15:30	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	6	5	theme	ristocetin	794:803	arg1	presence					782:789	the presence	778:789	the presence of ristocetin (P<0.05)	778:812	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	9	6	theme	VWF	1407:1409	arg1	clearance					1394:1402	MGL-mediated clearance	1381:1402	MGL-mediated clearance of VWF	1381:1409	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	8	7	theme	pathological	1141:1152	arg1	clearance					1165:1173	pathological, increased clearance	1141:1173	clearance	1165:1173	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	8	8	theme	Willebrand	1203:1212	arg1	disease					1214:1220	von Willebrand disease	1199:1220	von Willebrand disease	1199:1220	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	4	9	from	increase	489:496	arg1	P<0.05					539:544	P<0.05	539:544	P<0.05	539:544	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	4	9	from	increase	489:496	arg1	levels					531:536	endogenous plasma VWF antigen levels	501:536	endogenous plasma VWF antigen levels (P<0.05)	501:545	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	2	10	theme	VWF	275:277	arg1	clearance					279:287	hyposialylated VWF clearance	260:287	hyposialylated VWF clearance	260:287	A role for macrophage galactose lectin (MGL) in regulating hyposialylated VWF clearance has recently been proposed.
33763999	8	11	theme	increased	1155:1163	arg1	clearance					1165:1173	pathological, increased clearance	1141:1173	clearance	1165:1173	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	12	12	dep	regulating	1805:1814	arg1	addition					1793:1800	addition	1793:1800	addition	1793:1800	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	10	13	theme	VWF	1560:1562	arg1	clearance					1547:1555	the increased clearance	1533:1555	the increased clearance of VWF following loss of O-sialylation	1533:1594	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	6	14	from	site	847:850	arg1	A1					855:856	A1	855:856	A1	855:856	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	7	15	link	O-linked	971:978	arg1	glycans					980:986	VWF O-linked glycans	967:986	VWF O-linked glycans	967:986	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	9	16	from	loss	1260:1263	arg1	O-glycans					1297:1305	O-glycans	1297:1305	O-glycans	1297:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	4	17	theme	extended	449:456	arg1	time					473:476	a significantly extended mean residence time	433:476	a significantly extended mean residence time	433:476	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	10	18	gly	asialoglycoprotein	1451:1468	arg1	asialoglycoprotein					1451:1468	the asialoglycoprotein receptor	1447:1477	the asialoglycoprotein receptor (ASGPR)	1447:1485	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	10	19	theme	O-sialylation	1582:1594	arg1	loss					1574:1577	loss	1574:1577	loss of O-sialylation	1574:1594	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	12	20	theme	sialylated	1934:1943	arg1	VWF					1945:1947	aberrantly sialylated VWF	1923:1947	aberrantly sialylated VWF	1923:1947	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	4	21	theme	residence	463:471	arg1	time					473:476	a significantly extended mean residence time	433:476	a significantly extended mean residence time	433:476	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	7	22	theme	A1	1020:1021	arg1	domain					1023:1028	the A1 domain	1016:1028	the A1 domain	1016:1028	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	2	23	theme	galactose	223:231	arg1	MGL					241:243	MGL	241:243	MGL	241:243	A role for macrophage galactose lectin (MGL) in regulating hyposialylated VWF clearance has recently been proposed.
33763999	2	23	theme	galactose	223:231	arg1	lectin					233:238	macrophage galactose lectin	212:238	macrophage galactose lectin (MGL)	212:244	A role for macrophage galactose lectin (MGL) in regulating hyposialylated VWF clearance has recently been proposed.
33763999	5	24	theme	MGL	679:681	arg1	interaction					683:693	the MGL interaction	675:693	the MGL interaction	675:693	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	7	25	theme	O-linked	971:978	arg1	glycans					980:986	VWF O-linked glycans	967:986	VWF O-linked glycans	967:986	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	8	26	with	patients	1185:1192	arg1	disease					1214:1220	von Willebrand disease	1199:1220	von Willebrand disease	1199:1220	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	1	27	theme	von	172:174	arg1	VWF					195:197	VWF	195:197	VWF	195:197	Terminal sialylation determines the plasma half-life of von Willebrand factor (VWF).
33763999	1	27	theme	von	172:174	arg1	factor					187:192	von Willebrand factor	172:192	von Willebrand factor (VWF)	172:198	Terminal sialylation determines the plasma half-life of von Willebrand factor (VWF).
33763999	4	28	theme	endogenous	501:510	arg1	P<0.05					539:544	P<0.05	539:544	P<0.05	539:544	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	4	28	theme	endogenous	501:510	arg1	levels					531:536	endogenous plasma VWF antigen levels	501:536	endogenous plasma VWF antigen levels (P<0.05)	501:545	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	0	29	theme	galactose	79:87	arg1	clearance					105:113	macrophage galactose lectin-mediated clearance	68:113	macrophage galactose lectin-mediated clearance	68:113	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	1	30	theme	factor	187:192	arg1	half-life					159:167	the plasma half-life	148:167	the plasma half-life of von Willebrand factor (VWF)	148:198	Terminal sialylation determines the plasma half-life of von Willebrand factor (VWF).
33763999	0	31	from	Sialylation	0:10	arg1	glycans					24:30	O-linked glycans	15:30	O-linked glycans	15:30	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	9	32	theme	α2-3	1268:1271	arg1	sialylation					1280:1290	α2-3 linked sialylation	1268:1290	α2-3 linked sialylation from O-glycans	1268:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	7	33	theme	different	924:932	arg1	glycoforms					938:947	different VWF glycoforms	924:947	different VWF glycoforms	924:947	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	9	34	theme	sialylation	1280:1290	arg1	loss					1260:1263	specific loss	1251:1263	specific loss of α2-3 linked sialylation from O-glycans	1251:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	12	35	theme	disease	1987:1993	arg1	pathogenesis					1956:1967	the pathogenesis	1952:1967	the pathogenesis of von Willebrand disease	1952:1993	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	3	36	theme	VWF	383:385	arg1	clearance					387:395	physiological plasma VWF clearance	362:395	physiological plasma VWF clearance	362:395	In this study, we showed that MGL influences physiological plasma VWF clearance.
33763999	5	37	theme	truncations	570:580	arg1	series					556:561	a series	554:561	a series of VWF truncations	554:580	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	3	38	theme	physiological	362:374	arg1	clearance					387:395	physiological plasma VWF clearance	362:395	physiological plasma VWF clearance	362:395	In this study, we showed that MGL influences physiological plasma VWF clearance.
33763999	0	39	theme	O-linked	15:22	arg1	glycans					24:30	O-linked glycans	15:30	O-linked glycans	15:30	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	10	40	theme	asialoglycoprotein	1451:1468	arg1	receptor					1470:1477	the asialoglycoprotein receptor	1447:1477	the asialoglycoprotein receptor (ASGPR)	1447:1485	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	10	40	theme	asialoglycoprotein	1451:1468	arg1	ASGPR					1480:1484	ASGPR	1480:1484	ASGPR	1480:1484	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	9	41	theme	increased	1327:1335	arg1	MGL-binding					1337:1347	markedly increased MGL-binding	1318:1347	markedly increased MGL-binding	1318:1347	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	12	42	theme	MGL	1849:1851	arg1	receptor					1853:1860	the MGL receptor	1845:1860	the MGL receptor	1845:1860	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	6	43	theme	MGL-binding	835:845	arg1	site					847:850	the MGL-binding site	831:850	the MGL-binding site in A1	831:856	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	6	43	theme	MGL-binding	835:845	arg1	accessible					871:880	accessible	871:880	accessible	871:880	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	11	44	theme	sialylation	1651:1661	arg1	loss					1634:1637	loss	1634:1637	loss of N-linked sialylation from VWF	1634:1670	Conversely however, we observed that loss of N-linked sialylation from VWF drives enhanced circulatory clearance predominantly via the ASGPR.
33763999	0	45	theme	Willebrand	45:54	arg1	factor					56:61	von Willebrand factor	41:61	von Willebrand factor	41:61	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	5	46	theme	A1	616:617	arg1	domain					619:624	the A1 domain	612:624	the A1 domain of VWF	612:631	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	5	46	theme	A1	616:617	arg1	VWF					629:631	VWF	629:631	VWF	629:631	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	5	46	theme	A1	616:617	arg1	responsible					650:660	responsible	650:660	responsible	650:660	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	12	47	theme	VWF	1830:1832	arg1	clearance					1834:1842	physiological VWF clearance	1816:1842	physiological VWF clearance	1816:1842	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	11	48	theme	enhanced	1679:1686	arg1	clearance					1700:1708	enhanced circulatory clearance	1679:1708	enhanced circulatory clearance	1679:1708	Conversely however, we observed that loss of N-linked sialylation from VWF drives enhanced circulatory clearance predominantly via the ASGPR.
33763999	11	49	link	N-linked	1642:1649	arg1	sialylation					1651:1661	N-linked sialylation	1642:1661	N-linked sialylation	1642:1661	Conversely however, we observed that loss of N-linked sialylation from VWF drives enhanced circulatory clearance predominantly via the ASGPR.
33763999	6	50	from	VWF	894:896	arg1	site					847:850	the MGL-binding site	831:850	the MGL-binding site in A1	831:856	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	6	50	from	VWF	894:896	arg1	accessible					871:880	accessible	871:880	accessible	871:880	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	5	51	theme	VWF	629:631	arg1	domain					619:624	the A1 domain	612:624	the A1 domain of VWF	612:631	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	5	51	theme	VWF	629:631	arg1	VWF					629:631	VWF	629:631	VWF	629:631	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	5	51	theme	VWF	629:631	arg1	responsible					650:660	responsible	650:660	responsible	650:660	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	9	52	theme	MGL-mediated	1381:1392	arg1	clearance					1394:1402	MGL-mediated clearance	1381:1402	MGL-mediated clearance of VWF	1381:1409	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	4	53	theme	MGL	398:400	arg1	inhibition					402:411	MGL inhibition	398:411	MGL inhibition	398:411	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	7	54	gly	glycoforms	938:947	arg1	VWF					934:936	different VWF glycoforms	924:947	different VWF glycoforms	924:947	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	10	55	theme	increased	1537:1545	arg1	clearance					1547:1555	the increased clearance	1533:1555	the increased clearance of VWF following loss of O-sialylation	1533:1594	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	12	56	theme	Willebrand	1976:1985	arg1	disease					1987:1993	von Willebrand disease	1972:1993	von Willebrand disease	1972:1993	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	2	57	gly	hyposialylated	260:273	arg1	clearance					279:287	hyposialylated VWF clearance	260:287	hyposialylated VWF clearance	260:287	A role for macrophage galactose lectin (MGL) in regulating hyposialylated VWF clearance has recently been proposed.
33763999	8	58	theme	von	1199:1201	arg1	disease					1214:1220	von Willebrand disease	1199:1220	von Willebrand disease	1199:1220	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	7	59	theme	key	1038:1040	arg1	role					1042:1045	a key role	1036:1045	a key role	1036:1045	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	12	60	theme	VWF	1945:1947	arg1	clearance					1910:1918	enhanced clearance	1901:1918	enhanced clearance of aberrantly sialylated VWF	1901:1947	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	11	61	theme	circulatory	1688:1698	arg1	clearance					1700:1708	enhanced circulatory clearance	1679:1708	enhanced circulatory clearance	1679:1708	Conversely however, we observed that loss of N-linked sialylation from VWF drives enhanced circulatory clearance predominantly via the ASGPR.
33763999	4	62	from	time	473:476	arg1	P<0.05					539:544	P<0.05	539:544	P<0.05	539:544	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	4	62	from	time	473:476	arg1	levels					531:536	endogenous plasma VWF antigen levels	501:536	endogenous plasma VWF antigen levels (P<0.05)	501:545	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	12	63	gly	sialylated	1934:1943	arg1	VWF					1945:1947	aberrantly sialylated VWF	1923:1947	aberrantly sialylated VWF	1923:1947	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	4	64	theme	mean	458:461	arg1	time					473:476	a significantly extended mean residence time	433:476	a significantly extended mean residence time	433:476	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	7	65	theme	domain	1023:1028	arg1	end					1009:1011	either end	1002:1011	either end of the A1 domain	1002:1028	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	8	66	theme	VWF	1178:1180	arg1	clearance					1165:1173	pathological, increased clearance	1141:1173	clearance	1165:1173	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	4	67	theme	VWF	519:521	arg1	P<0.05					539:544	P<0.05	539:544	P<0.05	539:544	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	4	67	theme	VWF	519:521	arg1	levels					531:536	endogenous plasma VWF antigen levels	501:536	endogenous plasma VWF antigen levels (P<0.05)	501:545	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	2	68	theme	hyposialylated	260:273	arg1	clearance					279:287	hyposialylated VWF clearance	260:287	hyposialylated VWF clearance	260:287	A role for macrophage galactose lectin (MGL) in regulating hyposialylated VWF clearance has recently been proposed.
33763999	1	69	theme	plasma	152:157	arg1	half-life					159:167	the plasma half-life	148:167	the plasma half-life of von Willebrand factor (VWF)	148:198	Terminal sialylation determines the plasma half-life of von Willebrand factor (VWF).
33763999	0	70	theme	macrophage	68:77	arg1	clearance					105:113	macrophage galactose lectin-mediated clearance	68:113	macrophage galactose lectin-mediated clearance	68:113	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	4	71	theme	plasma	512:517	arg1	P<0.05					539:544	P<0.05	539:544	P<0.05	539:544	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	4	71	theme	plasma	512:517	arg1	levels					531:536	endogenous plasma VWF antigen levels	501:536	endogenous plasma VWF antigen levels (P<0.05)	501:545	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	0	72	theme	lectin-mediated	89:103	arg1	clearance					105:113	macrophage galactose lectin-mediated clearance	68:113	macrophage galactose lectin-mediated clearance	68:113	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	9	73	theme	specific	1251:1258	arg1	loss					1260:1263	specific loss	1251:1263	specific loss of α2-3 linked sialylation from O-glycans	1251:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	7	74	theme	VWF	967:969	arg1	glycans					980:986	VWF O-linked glycans	967:986	VWF O-linked glycans	967:986	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	1	75	theme	Willebrand	176:185	arg1	VWF					195:197	VWF	195:197	VWF	195:197	Terminal sialylation determines the plasma half-life of von Willebrand factor (VWF).
33763999	1	75	theme	Willebrand	176:185	arg1	factor					187:192	von Willebrand factor	172:192	von Willebrand factor (VWF)	172:198	Terminal sialylation determines the plasma half-life of von Willebrand factor (VWF).
33763999	4	76	theme	antigen	523:529	arg1	P<0.05					539:544	P<0.05	539:544	P<0.05	539:544	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	4	76	theme	antigen	523:529	arg1	levels					531:536	endogenous plasma VWF antigen levels	501:536	endogenous plasma VWF antigen levels (P<0.05)	501:545	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	12	77	theme	enhanced	1901:1908	arg1	clearance					1910:1918	enhanced clearance	1901:1918	enhanced clearance of aberrantly sialylated VWF	1901:1947	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	10	78	contain	have	1496:1499	arg1	receptor					1470:1477	the asialoglycoprotein receptor	1447:1477	the asialoglycoprotein receptor (ASGPR)	1447:1485	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	10	78	contain	have	1496:1499	arg2	role					1515:1518	a significant role	1501:1518	a significant role	1501:1518	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	10	78	contain	have	1496:1499	arg1	ASGPR					1480:1484	ASGPR	1480:1484	ASGPR	1480:1484	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	4	79	theme	3-fold	482:487	arg1	increase					489:496	3-fold increase	482:496	3-fold increase in endogenous plasma VWF antigen levels (P<0.05)	482:545	MGL inhibition was associated with a significantly extended mean residence time and 3-fold increase in endogenous plasma VWF antigen levels (P<0.05).
33763999	9	80	theme	linked	1273:1278	arg1	sialylation					1280:1290	α2-3 linked sialylation	1268:1290	α2-3 linked sialylation from O-glycans	1268:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	7	81	theme	VWF	934:936	arg1	glycoforms					938:947	different VWF glycoforms	924:947	different VWF glycoforms	924:947	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	11	82	from	VWF	1668:1670	arg1	loss					1634:1637	loss	1634:1637	loss of N-linked sialylation from VWF	1634:1670	Conversely however, we observed that loss of N-linked sialylation from VWF drives enhanced circulatory clearance predominantly via the ASGPR.
33763999	9	83	from	O-glycans	1297:1305	arg1	loss					1260:1263	specific loss	1251:1263	specific loss of α2-3 linked sialylation from O-glycans	1251:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	9	83	from	O-glycans	1297:1305	arg1	sialylation					1280:1290	α2-3 linked sialylation	1268:1290	α2-3 linked sialylation from O-glycans	1268:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	5	84	theme	VWF	566:568	arg1	truncations					570:580	VWF truncations	566:580	VWF truncations	566:580	Using a series of VWF truncations, we further demonstrated that the A1 domain of VWF is predominantly responsible for enabling the MGL interaction.
33763999	9	85	link	linked	1273:1278	arg1	sialylation					1280:1290	α2-3 linked sialylation	1268:1290	α2-3 linked sialylation from O-glycans	1268:1305	Herein, we demonstrate that specific loss of α2-3 linked sialylation from O-glycans results in markedly increased MGL-binding in vitro, and markedly enhanced MGL-mediated clearance of VWF in vivo.
33763999	7	86	theme	Additional	899:908	arg1	studies					910:916	Additional studies	899:916	Additional studies using different VWF glycoforms	899:947	Additional studies using different VWF glycoforms demonstrated that VWF O-linked glycans, clustered at either end of the A1 domain, play a key role in protecting VWF against MGLmediated clearance.
33763999	1	87	theme	Terminal	116:123	arg1	sialylation					125:135	Terminal sialylation	116:135	Terminal sialylation	116:135	Terminal sialylation determines the plasma half-life of von Willebrand factor (VWF).
33763999	3	88	theme	plasma	376:381	arg1	clearance					387:395	physiological plasma VWF clearance	362:395	physiological plasma VWF clearance	362:395	In this study, we showed that MGL influences physiological plasma VWF clearance.
33763999	0	89	theme	von	41:43	arg1	factor					56:61	von Willebrand factor	41:61	von Willebrand factor	41:61	Sialylation on O-linked glycans protects von Willebrand factor from macrophage galactose lectin-mediated clearance.
33763999	11	90	theme	N-linked	1642:1649	arg1	sialylation					1651:1661	N-linked sialylation	1642:1661	N-linked sialylation	1642:1661	Conversely however, we observed that loss of N-linked sialylation from VWF drives enhanced circulatory clearance predominantly via the ASGPR.
33763999	12	91	with	tandem	1871:1876	arg1	ASGPR					1883:1887	ASGPR	1883:1887	ASGPR	1883:1887	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	12	92	theme	physiological	1816:1828	arg1	clearance					1834:1842	physiological VWF clearance	1816:1842	physiological VWF clearance	1816:1842	Collectively, our data support the hypothesis that in addition to regulating physiological VWF clearance, the MGL receptor works in tandem with ASGPR to modulate enhanced clearance of aberrantly sialylated VWF in the pathogenesis of von Willebrand disease.
33763999	6	93	from	accessible	871:880	arg1	VWF					894:896	globular VWF	885:896	globular VWF	885:896	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	2	94	theme	macrophage	212:221	arg1	MGL					241:243	MGL	241:243	MGL	241:243	A role for macrophage galactose lectin (MGL) in regulating hyposialylated VWF clearance has recently been proposed.
33763999	2	94	theme	macrophage	212:221	arg1	lectin					233:238	macrophage galactose lectin	212:238	macrophage galactose lectin (MGL)	212:244	A role for macrophage galactose lectin (MGL) in regulating hyposialylated VWF clearance has recently been proposed.
33763999	6	95	theme	VWF-A1-A2-A3	728:739	arg1	Binding					696:702	Binding	696:702	Binding of both full-length and VWF-A1-A2-A3 to MGL	696:746	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	8	96	theme	Reduced	1096:1102	arg1	sialylation					1104:1114	Reduced sialylation	1096:1114	Reduced sialylation	1096:1114	Reduced sialylation has been associated with pathological, increased clearance of VWF in patients with von Willebrand disease.
33763999	6	97	dep	fully	865:869	arg1	not					861:863	not	861:863	not	861:863	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
33763999	10	98	theme	significant	1503:1513	arg1	role					1515:1518	a significant role	1501:1518	a significant role	1501:1518	Our data further show that the asialoglycoprotein receptor (ASGPR) does not have a significant role in mediating the increased clearance of VWF following loss of O-sialylation.
33763999	6	99	theme	full-length	712:722	arg1	Binding					696:702	Binding	696:702	Binding of both full-length and VWF-A1-A2-A3 to MGL	696:746	Binding of both full-length and VWF-A1-A2-A3 to MGL was significantly enhanced in the presence of ristocetin (P<0.05), suggesting that the MGL-binding site in A1 is not fully accessible in globular VWF.
35688738	3	0	theme	16S	873:875	arg1	sequencing					898:907	16S full-length amplicon sequencing	873:907	16S full-length amplicon sequencing	873:907	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	4	1	theme	tight	946:950	arg1	proteins					961:968	tight junction proteins	946:968	tight junction proteins	946:968	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	0	2	theme	dairy	176:180	arg1	cattle					182:187	dairy cattle	176:187	dairy cattle	176:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	1	3	theme	small	436:440	arg1	intestines					442:451	the small intestines	432:451	the small intestines of dairy cattle fed a high-grain (HG) diet	432:494	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	13	4	theme	bacterial	2579:2587	arg1	community					2589:2597	mucosa-associated bacterial community	2561:2597	mucosa-associated bacterial community	2561:2597	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	7	5	theme	HG	1409:1410	arg1	diet					1412:1415	the HG diet	1405:1415	the HG diet	1405:1415	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	5	6	dep	jejunum	1232:1238	arg1	the					1228:1230	the	1228:1230	the	1228:1230	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	4	7	theme	proteins	961:968	arg1	expression					932:941	the gene expression	923:941	the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium	923:1030	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	10	8	contain	had	2031:2033	arg2	concentrations					2042:2055	higher concentrations	2035:2055	higher concentrations of lipopolysaccharides	2035:2078	Additionally, the group fed an HG diet had higher concentrations of lipopolysaccharides in the jejunal and ileal digesta.
35688738	10	8	contain	had	2031:2033	arg1	group					2010:2014	the group	2006:2014	the group fed an HG diet	2006:2029	Additionally, the group fed an HG diet had higher concentrations of lipopolysaccharides in the jejunal and ileal digesta.
35688738	12	9	theme	ileal	2460:2464	arg1	epithelium					2466:2475	the ileal epithelium	2456:2475	the ileal epithelium	2456:2475	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	11	10	theme	claudin-1	2238:2246	arg1	downregulation					2138:2151	a downregulation	2136:2151	a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1	2136:2267	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	5	11	from	composition	1170:1180	arg1	ileum					1244:1248	ileum	1244:1248	ileum	1244:1248	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	5	11	from	composition	1170:1180	arg1	jejunum					1232:1238	jejunum	1232:1238	jejunum	1232:1238	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	10	12	theme	HG	2023:2024	arg1	diet					2026:2029	an HG diet	2020:2029	an HG diet	2020:2029	Additionally, the group fed an HG diet had higher concentrations of lipopolysaccharides in the jejunal and ileal digesta.
35688738	1	13	theme	cattle	462:467	arg1	intestines					442:451	the small intestines	432:451	the small intestines of dairy cattle fed a high-grain (HG) diet	432:494	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	13	14	theme	lipopolysaccharide	2531:2548	arg1	levels					2550:2555	lipopolysaccharide levels	2531:2555	lipopolysaccharide levels	2531:2555	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	7	15	theme	genus	1392:1396	arg1	level					1398:1402	the genus level	1388:1402	the genus level	1388:1402	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	2	16	theme	%	667:667	arg1	basis					693:697	dry matter basis	682:697	dry matter basis	682:697	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	16	theme	%	667:667	arg1	concentrate					669:679	60% concentrate	665:679	60% concentrate	665:679	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	11	17	from	epithelium	2212:2221	arg1	downregulation					2138:2151	a downregulation	2136:2151	a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1	2136:2267	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	2	18	theme	Holstein	532:539	arg1	cows					541:544	12 ruminally cannulated Holstein cows	508:544	12 ruminally cannulated Holstein cows in mid-lactation	508:561	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	4	19	theme	small	1004:1008	arg1	epithelium					1021:1030	the small intestinal epithelium	1000:1030	the small intestinal epithelium	1000:1030	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	13	20	theme	HG	2712:2713	arg1	feeding					2715:2721	HG feeding	2712:2721	HG feeding	2712:2721	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	3	21	from	changes	795:801	arg1	communities					855:865	the small intestinal mucosa-associated bacterial communities	806:865	the small intestinal mucosa-associated bacterial communities	806:865	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	2	22	theme	matter	686:691	arg1	basis					693:697	dry matter basis	682:697	dry matter basis	682:697	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	22	theme	matter	686:691	arg1	concentrate					669:679	60% concentrate	665:679	60% concentrate	665:679	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	0	23	theme	cytokines	140:148	arg1	Effects					0:6	Effects	0:6	Effects of high-grain diet feeding on mucosa-associated bacterial community	0:74	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	0	23	theme	cytokines	140:148	arg1	expression					85:94	gene expression	80:94	gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle	80:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	1	24	theme	proteins	393:400	arg1	morphology					310:319	the morphology	306:319	the morphology of the small intestinal epithelia	306:353	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	24	theme	proteins	393:400	arg1	composition					252:262	the composition	248:262	the composition of mucosa-associated bacterial community	248:303	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	24	theme	proteins	393:400	arg1	expressions					369:379	the gene expressions	360:379	the gene expressions of junction proteins and inflammatory cytokines	360:427	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	25	theme	study	212:216	arg1	objective					194:202	The objective	190:202	The objective of this study	190:216	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	12	26	theme	IFN-γ	2447:2451	arg1	expression					2424:2433	a higher expression	2415:2433	a higher expression of IL-2 and IFN-γ in the ileal epithelium	2415:2475	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	1	27	theme	bacterial	285:293	arg1	community					295:303	mucosa-associated bacterial community	267:303	mucosa-associated bacterial community	267:303	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	9	28	theme	xenobiotics	1884:1894	arg1	biodegradation					1866:1879	biodegradation	1866:1879	biodegradation of xenobiotics in the jejunal mucosa	1866:1916	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	1	29	from	changes	237:243	arg1	morphology					310:319	the morphology	306:319	the morphology of the small intestinal epithelia	306:353	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	29	from	changes	237:243	arg1	composition					252:262	the composition	248:262	the composition of mucosa-associated bacterial community	248:303	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	29	from	changes	237:243	arg1	intestines					442:451	the small intestines	432:451	the small intestines of dairy cattle fed a high-grain (HG) diet	432:494	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	29	from	changes	237:243	arg1	expressions					369:379	the gene expressions	360:379	the gene expressions of junction proteins and inflammatory cytokines	360:427	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	30	theme	inflammatory	406:417	arg1	cytokines					419:427	inflammatory cytokines	406:427	inflammatory cytokines	406:427	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	8	31	theme	ileal	1713:1717	arg1	mucosa					1719:1724	the ileal mucosa	1709:1724	the ileal mucosa	1709:1724	Compared with the CON group, the proportions of Acetitomaculum in both the jejunal and the ileal mucosa were higher in the HG group.
35688738	10	32	theme	lipopolysaccharides	2060:2078	arg1	concentrations					2042:2055	higher concentrations	2035:2055	higher concentrations of lipopolysaccharides	2035:2078	Additionally, the group fed an HG diet had higher concentrations of lipopolysaccharides in the jejunal and ileal digesta.
35688738	11	33	from	expression	2165:2174	arg1	epithelium					2212:2221	the jejunal epithelium	2200:2221	the jejunal epithelium	2200:2221	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	3	34	theme	feeding	728:734	arg1	trial					736:740	the feeding trial	724:740	the feeding trial	724:740	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	2	35	from	total	499:503	arg1	mid-lactation					549:561	mid-lactation	549:561	mid-lactation	549:561	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	1	36	theme	small	328:332	arg1	epithelia					345:353	the small intestinal epithelia	324:353	the small intestinal epithelia	324:353	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	5	37	theme	multidimensional	1104:1119	arg1	plots					1129:1133	nonmetric multidimensional scaling plots	1094:1133	nonmetric multidimensional scaling plots	1094:1133	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	2	38	theme	HG	656:657	arg1	diet					659:662	an HG diet	653:662	an HG diet (60% concentrate, dry matter basis)	653:698	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	0	39	theme	gene	80:83	arg1	expression					85:94	gene expression	80:94	gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle	80:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	1	40	theme	epithelia	345:353	arg1	morphology					310:319	the morphology	306:319	the morphology of the small intestinal epithelia	306:353	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	40	theme	epithelia	345:353	arg1	composition					252:262	the composition	248:262	the composition of mucosa-associated bacterial community	248:303	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	40	theme	epithelia	345:353	arg1	expressions					369:379	the gene expressions	360:379	the gene expressions of junction proteins and inflammatory cytokines	360:427	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	11	41	theme	expression	2165:2174	arg1	downregulation					2138:2151	a downregulation	2136:2151	a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1	2136:2267	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	8	42	theme	HG	1745:1746	arg1	group					1748:1752	the HG group	1741:1752	the HG group	1741:1752	Compared with the CON group, the proportions of Acetitomaculum in both the jejunal and the ileal mucosa were higher in the HG group.
35688738	14	43	theme	essential	2803:2811	arg1	benefits					2820:2827	essential health benefits	2803:2827	essential health benefits to host dairy cattle	2803:2848	These findings suggest that microbiota residing in the small intestine provide essential health benefits to host dairy cattle.
35688738	7	44	theme	duodenal	1517:1524	arg1	mucosa					1526:1531	the duodenal mucosa	1513:1531	the duodenal mucosa	1513:1531	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	0	45	from	Effects	0:6	arg1	community					66:74	mucosa-associated bacterial community	38:74	mucosa-associated bacterial community	38:74	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	0	45	from	Effects	0:6	arg1	intestine					163:171	the small intestine	153:171	the small intestine of dairy cattle	153:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	2	46	theme	40	615:616	arg1	%					617:617	%	617:617	%	617:617	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	5	47	theme	bacterial	1203:1211	arg1	communities					1213:1223	mucosa-associated bacterial communities	1185:1223	mucosa-associated bacterial communities	1185:1223	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	13	48	theme	community	2589:2597	arg1	alteration					2517:2526	the alteration	2513:2526	the alteration of lipopolysaccharide levels and mucosa-associated bacterial community	2513:2597	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	9	49	theme	jejunal	1903:1909	arg1	mucosa					1911:1916	the jejunal mucosa	1899:1916	the jejunal mucosa	1899:1916	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	6	50	from	numbers	1285:1291	arg1	community					1359:1367	the mucosa-associated bacterial community	1327:1367	the mucosa-associated bacterial community in the jejunum	1327:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	13	51	theme	epithelial	2659:2668	arg1	cytokines					2670:2678	the epithelial cytokines	2655:2678	the epithelial cytokines	2655:2678	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	2	52	dep	diet	659:662	arg1	basis					693:697	dry matter basis	682:697	dry matter basis	682:697	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	52	dep	diet	659:662	arg1	concentrate					669:679	60% concentrate	665:679	60% concentrate	665:679	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	9	53	theme	related	1945:1951	arg1	genes					1939:1943	genes	1939:1943	genes related to immune disease in the ileal mucosa	1939:1989	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	0	54	theme	junction	105:112	arg1	proteins					114:121	tight junction proteins	99:121	tight junction proteins	99:121	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	1	55	theme	gene	364:367	arg1	expressions					369:379	the gene expressions	360:379	the gene expressions of junction proteins and inflammatory cytokines	360:427	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	4	56	theme	gene	927:930	arg1	expression					932:941	the gene expression	923:941	the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium	923:1030	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	2	57	theme	dry	632:634	arg1	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	57	theme	dry	632:634	arg1	concentrate					619:629	40% concentrate	615:629	40% concentrate	615:629	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	12	58	theme	IL-2	2368:2371	arg1	expression					2347:2356	the mRNA expression	2338:2356	the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium	2338:2408	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	1	59	theme	mucosa-associated	267:283	arg1	community					295:303	mucosa-associated bacterial community	267:303	mucosa-associated bacterial community	267:303	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	11	60	from	downregulation	2138:2151	arg1	epithelium					2212:2221	the jejunal epithelium	2200:2221	the jejunal epithelium	2200:2221	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	0	61	theme	high-grain	11:20	arg1	feeding					27:33	high-grain diet feeding	11:33	high-grain diet feeding	11:33	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	1	62	theme	community	295:303	arg1	morphology					310:319	the morphology	306:319	the morphology of the small intestinal epithelia	306:353	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	62	theme	community	295:303	arg1	composition					252:262	the composition	248:262	the composition of mucosa-associated bacterial community	248:303	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	62	theme	community	295:303	arg1	expressions					369:379	the gene expressions	360:379	the gene expressions of junction proteins and inflammatory cytokines	360:427	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	7	63	theme	Lachnospiraceae	1479:1493	arg1	abundance					1431:1439	the abundance	1427:1439	the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa	1427:1531	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	7	64	theme	jejunal	1606:1612	arg1	mucosa					1614:1619	the jejunal mucosa	1602:1619	the jejunal mucosa	1602:1619	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	11	65	theme	occludin	2179:2186	arg1	expression					2165:2174	the mRNA expression	2156:2174	the mRNA expression of occludin and ZO-1 in the jejunal epithelium	2156:2221	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	9	66	theme	ileal	1978:1982	arg1	mucosa					1984:1989	the ileal mucosa	1974:1989	the ileal mucosa	1974:1989	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	0	67	theme	feeding	27:33	arg1	Effects					0:6	Effects	0:6	Effects of high-grain diet feeding on mucosa-associated bacterial community	0:74	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	0	67	theme	feeding	27:33	arg1	expression					85:94	gene expression	80:94	gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle	80:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	7	68	theme	Succinivibrionaceae	1455:1473	arg1	abundance					1431:1439	the abundance	1427:1439	the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa	1427:1531	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	3	69	theme	bacterial	845:853	arg1	communities					855:865	the small intestinal mucosa-associated bacterial communities	806:865	the small intestinal mucosa-associated bacterial communities	806:865	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	1	70	theme	HG	487:488	arg1	diet					491:494	a high-grain (HG) diet	473:494	a high-grain (HG) diet	473:494	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	0	71	theme	mucosa-associated	38:54	arg1	community					66:74	mucosa-associated bacterial community	38:74	mucosa-associated bacterial community	38:74	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	6	72	from	units	1318:1322	arg1	community					1359:1367	the mucosa-associated bacterial community	1327:1367	the mucosa-associated bacterial community in the jejunum	1327:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	3	73	theme	intestinal	816:825	arg1	communities					855:865	the small intestinal mucosa-associated bacterial communities	806:865	the small intestinal mucosa-associated bacterial communities	806:865	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	13	74	from	expression	2641:2650	arg1	jejunum					2687:2693	jejunum	2687:2693	jejunum	2687:2693	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	13	74	from	expression	2641:2650	arg1	ileum					2699:2703	ileum	2699:2703	ileum	2699:2703	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	4	75	theme	cytokines	987:995	arg1	expression					932:941	the gene expression	923:941	the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium	923:1030	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	12	76	theme	IFN-γ	2378:2382	arg1	expression					2347:2356	the mRNA expression	2338:2356	the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium	2338:2408	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	5	77	theme	HG	1150:1151	arg1	diet					1153:1156	an HG diet	1147:1156	an HG diet	1147:1156	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	3	78	theme	full-length	877:887	arg1	sequencing					898:907	16S full-length amplicon sequencing	873:907	16S full-length amplicon sequencing	873:907	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	11	79	theme	jejunal	2204:2210	arg1	epithelium					2212:2221	the jejunal epithelium	2200:2221	the jejunal epithelium	2200:2221	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	11	80	theme	mRNA	2160:2163	arg1	expression					2165:2174	the mRNA expression	2156:2174	the mRNA expression of occludin and ZO-1 in the jejunal epithelium	2156:2221	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	14	81	theme	dairy	2837:2841	arg1	cattle					2843:2848	host dairy cattle	2832:2848	host dairy cattle	2832:2848	These findings suggest that microbiota residing in the small intestine provide essential health benefits to host dairy cattle.
35688738	7	82	dep	Lachnospiraceae	1479:1493	arg1	sedis					1504:1508	Lachnospiraceae incertae sedis	1479:1508	Lachnospiraceae incertae sedis	1479:1508	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	6	83	theme	bacterial	1349:1357	arg1	community					1359:1367	the mucosa-associated bacterial community	1327:1367	the mucosa-associated bacterial community in the jejunum	1327:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	4	84	theme	junction	952:959	arg1	proteins					961:968	tight junction proteins	946:968	tight junction proteins	946:968	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	0	85	theme	cattle	182:187	arg1	intestine					163:171	the small intestine	153:171	the small intestine of dairy cattle	153:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	13	86	theme	mucosa-associated	2561:2577	arg1	community					2589:2597	mucosa-associated bacterial community	2561:2597	mucosa-associated bacterial community	2561:2597	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	5	87	theme	plots	1129:1133	arg1	results					1083:1089	The results	1079:1089	The results of nonmetric multidimensional scaling plots	1079:1133	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	9	88	theme	HG	1814:1815	arg1	diet					1817:1820	the HG diet	1810:1820	the HG diet	1810:1820	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	2	89	from	cows	541:544	arg1	mid-lactation					549:561	mid-lactation	549:561	mid-lactation	549:561	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	14	90	theme	health	2813:2818	arg1	benefits					2820:2827	essential health benefits	2803:2827	essential health benefits to host dairy cattle	2803:2848	These findings suggest that microbiota residing in the small intestine provide essential health benefits to host dairy cattle.
35688738	8	91	theme	CON	1640:1642	arg1	group					1644:1648	the CON group	1636:1648	the CON group	1636:1648	Compared with the CON group, the proportions of Acetitomaculum in both the jejunal and the ileal mucosa were higher in the HG group.
35688738	2	92	theme	dry	682:684	arg1	basis					693:697	dry matter basis	682:697	dry matter basis	682:697	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	92	theme	dry	682:684	arg1	concentrate					669:679	60% concentrate	665:679	60% concentrate	665:679	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	93	from	mid-lactation	549:561	arg1	total					499:503	A total	497:503	A total of 12 ruminally cannulated Holstein cows in mid-lactation	497:561	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	1	94	theme	dairy	456:460	arg1	cattle					462:467	dairy cattle	456:467	dairy cattle fed a high-grain (HG) diet	456:494	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	6	95	theme	mucosa-associated	1331:1347	arg1	community					1359:1367	the mucosa-associated bacterial community	1327:1367	the mucosa-associated bacterial community in the jejunum	1327:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	4	96	theme	quantitative	1061:1072	arg1	PCR					1074:1076	real-time quantitative PCR	1051:1076	real-time quantitative PCR	1051:1076	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	6	97	theme	taxonomic	1308:1316	arg1	units					1318:1322	operational taxonomic units	1296:1322	operational taxonomic units in the mucosa-associated bacterial community in the jejunum	1296:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	14	98	theme	small	2779:2783	arg1	intestine					2785:2793	the small intestine	2775:2793	the small intestine	2775:2793	These findings suggest that microbiota residing in the small intestine provide essential health benefits to host dairy cattle.
35688738	13	99	theme	levels	2550:2555	arg1	alteration					2517:2526	the alteration	2513:2526	the alteration of lipopolysaccharide levels and mucosa-associated bacterial community	2513:2597	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	12	100	from	expression	2424:2433	arg1	epithelium					2466:2475	the ileal epithelium	2456:2475	the ileal epithelium	2456:2475	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	2	101	theme	conventional	590:601	arg1	diet					609:612	a conventional (CON) diet	588:612	a conventional (CON) diet (40% concentrate, dry matter basis)	588:648	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	101	theme	conventional	590:601	arg1	CON					604:606	CON	604:606	CON	604:606	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	4	102	theme	intestinal	1010:1019	arg1	epithelium					1021:1030	the small intestinal epithelium	1000:1030	the small intestinal epithelium	1000:1030	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	0	103	theme	inflammatory	127:138	arg1	cytokines					140:148	inflammatory cytokines	127:148	inflammatory cytokines	127:148	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	1	104	theme	high-grain	475:484	arg1	diet					491:494	a high-grain (HG) diet	473:494	a high-grain (HG) diet	473:494	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	8	105	theme	Acetitomaculum	1670:1683	arg1	proportions					1655:1665	the proportions	1651:1665	the proportions of Acetitomaculum in both the jejunal and the ileal mucosa	1651:1724	Compared with the CON group, the proportions of Acetitomaculum in both the jejunal and the ileal mucosa were higher in the HG group.
35688738	8	105	theme	Acetitomaculum	1670:1683	arg1	higher					1731:1736	higher	1731:1736	higher	1731:1736	Compared with the CON group, the proportions of Acetitomaculum in both the jejunal and the ileal mucosa were higher in the HG group.
35688738	7	106	theme	Selenomonas	1577:1587	arg1	proportions					1546:1556	the proportions	1542:1556	the proportions of Veillonella and Selenomonas	1542:1587	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	2	107	theme	cannulated	521:530	arg1	cows					541:544	12 ruminally cannulated Holstein cows	508:544	12 ruminally cannulated Holstein cows in mid-lactation	508:561	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	0	108	from	expression	85:94	arg1	community					66:74	mucosa-associated bacterial community	38:74	mucosa-associated bacterial community	38:74	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	0	108	from	expression	85:94	arg1	intestine					163:171	the small intestine	153:171	the small intestine of dairy cattle	153:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	10	109	theme	higher	2035:2040	arg1	concentrations					2042:2055	higher concentrations	2035:2055	higher concentrations of lipopolysaccharides	2035:2078	Additionally, the group fed an HG diet had higher concentrations of lipopolysaccharides in the jejunal and ileal digesta.
35688738	9	110	theme	genes	1849:1853	arg1	abundance					1836:1844	the abundance	1832:1844	the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa	1832:1916	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	9	110	theme	genes	1849:1853	arg1	abundance					1926:1934	the abundance	1922:1934	the abundance of genes related to immune disease in the ileal mucosa	1922:1989	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	1	111	theme	junction	384:391	arg1	proteins					393:400	junction proteins	384:400	junction proteins	384:400	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	9	112	theme	version	1778:1784	arg1	PICRUSt2					1768:1775	PICRUSt2	1768:1775	PICRUSt2 (version 2.2.0-b)	1768:1793	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	9	112	theme	version	1778:1784	arg1	2.2.0-b					1786:1792	version 2.2.0-b	1778:1792	version 2.2.0-b	1778:1792	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	7	113	theme	Veillonella	1561:1571	arg1	proportions					1546:1556	the proportions	1542:1556	the proportions of Veillonella and Selenomonas	1542:1587	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	9	114	from	biodegradation	1866:1879	arg1	mucosa					1911:1916	the jejunal mucosa	1899:1916	the jejunal mucosa	1899:1916	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	10	115	theme	jejunal	2087:2093	arg1	digesta					2105:2111	the jejunal and ileal digesta	2083:2111	the jejunal and ileal digesta	2083:2111	Additionally, the group fed an HG diet had higher concentrations of lipopolysaccharides in the jejunal and ileal digesta.
35688738	13	116	dep	jejunum	2687:2693	arg1	the					2683:2685	the	2683:2685	the	2683:2685	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	6	117	theme	operational	1296:1306	arg1	units					1318:1322	operational taxonomic units	1296:1322	operational taxonomic units in the mucosa-associated bacterial community in the jejunum	1296:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	0	118	theme	small	157:161	arg1	intestine					163:171	the small intestine	153:171	the small intestine of dairy cattle	153:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	12	119	theme	IL-1β	2361:2365	arg1	expression					2347:2356	the mRNA expression	2338:2356	the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium	2338:2408	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	11	120	theme	ileal	2276:2280	arg1	epithelium					2282:2291	the ileal epithelium	2272:2291	the ileal epithelium	2272:2291	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	5	121	theme	communities	1213:1223	arg1	composition					1170:1180	the composition	1166:1180	the composition of mucosa-associated bacterial communities in the jejunum and ileum	1166:1248	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	1	122	theme	cytokines	419:427	arg1	morphology					310:319	the morphology	306:319	the morphology of the small intestinal epithelia	306:353	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	122	theme	cytokines	419:427	arg1	composition					252:262	the composition	248:262	the composition of mucosa-associated bacterial community	248:303	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	1	122	theme	cytokines	419:427	arg1	expressions					369:379	the gene expressions	360:379	the gene expressions of junction proteins and inflammatory cytokines	360:427	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	12	123	theme	higher	2417:2422	arg1	expression					2424:2433	a higher expression	2415:2433	a higher expression of IL-2 and IFN-γ in the ileal epithelium	2415:2475	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	2	124	theme	cows	541:544	arg1	total					499:503	A total	497:503	A total of 12 ruminally cannulated Holstein cows in mid-lactation	497:561	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	125	theme	60	665:666	arg1	%					667:667	%	667:667	%	667:667	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	9	126	theme	related	1855:1861	arg1	genes					1849:1853	genes	1849:1853	genes related to biodegradation of xenobiotics in the jejunal mucosa	1849:1916	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	9	127	theme	immune	1956:1961	arg1	disease					1963:1969	immune disease	1956:1969	immune disease in the ileal mucosa	1956:1989	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	6	128	from	community	1359:1367	arg1	jejunum					1376:1382	the jejunum	1372:1382	the jejunum	1372:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	6	128	from	community	1359:1367	arg1	numbers					1285:1291	the numbers	1281:1291	the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum	1281:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	3	129	theme	trial	736:740	arg1	end					717:719	the end	713:719	the end of the feeding trial	713:740	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	10	130	theme	ileal	2099:2103	arg1	digesta					2105:2111	the jejunal and ileal digesta	2083:2111	the jejunal and ileal digesta	2083:2111	Additionally, the group fed an HG diet had higher concentrations of lipopolysaccharides in the jejunal and ileal digesta.
35688738	6	131	theme	units	1318:1322	arg1	numbers					1285:1291	the numbers	1281:1291	the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum	1281:1382	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	1	132	theme	intestinal	334:343	arg1	epithelia					345:353	the small intestinal epithelia	324:353	the small intestinal epithelia	324:353	The objective of this study was to investigate changes in the composition of mucosa-associated bacterial community, the morphology of the small intestinal epithelia, and the gene expressions of junction proteins and inflammatory cytokines in the small intestines of dairy cattle fed a high-grain (HG) diet.
35688738	14	133	theme	host	2832:2835	arg1	cattle					2843:2848	host dairy cattle	2832:2848	host dairy cattle	2832:2848	These findings suggest that microbiota residing in the small intestine provide essential health benefits to host dairy cattle.
35688738	2	134	dep	diet	609:612	arg1	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	134	dep	diet	609:612	arg1	concentrate					619:629	40% concentrate	615:629	40% concentrate	615:629	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	11	135	theme	HG	2118:2119	arg1	feeding					2121:2127	The HG feeding	2114:2127	The HG feeding	2114:2127	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	4	136	theme	real-time	1051:1059	arg1	PCR					1074:1076	real-time quantitative PCR	1051:1076	real-time quantitative PCR	1051:1076	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	2	137	theme	%	617:617	arg1	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	137	theme	%	617:617	arg1	concentrate					619:629	40% concentrate	615:629	40% concentrate	615:629	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	5	138	theme	mucosa-associated	1185:1201	arg1	communities					1213:1223	mucosa-associated bacterial communities	1185:1223	mucosa-associated bacterial communities	1185:1223	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	0	139	theme	tight	99:103	arg1	proteins					114:121	tight junction proteins	99:121	tight junction proteins	99:121	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	4	140	from	expression	932:941	arg1	epithelium					1021:1030	the small intestinal epithelium	1000:1030	the small intestinal epithelium	1000:1030	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	9	141	theme	genes	1939:1943	arg1	abundance					1836:1844	the abundance	1832:1844	the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa	1832:1916	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	9	141	theme	genes	1939:1943	arg1	abundance					1926:1934	the abundance	1922:1934	the abundance of genes related to immune disease in the ileal mucosa	1922:1989	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	0	142	theme	proteins	114:121	arg1	Effects					0:6	Effects	0:6	Effects of high-grain diet feeding on mucosa-associated bacterial community	0:74	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	0	142	theme	proteins	114:121	arg1	expression					85:94	gene expression	80:94	gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle	80:187	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	13	143	theme	cytokines	2670:2678	arg1	expression					2641:2650	the expression	2637:2650	the expression of the epithelial cytokines in the jejunum and ileum	2637:2703	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	12	144	from	expression	2347:2356	arg1	epithelium					2399:2408	the jejunal epithelium	2387:2408	the jejunal epithelium	2387:2408	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	12	145	theme	jejunal	2391:2397	arg1	epithelium					2399:2408	the jejunal epithelium	2387:2408	the jejunal epithelium	2387:2408	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	2	146	theme	matter	636:641	arg1	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	2	146	theme	matter	636:641	arg1	concentrate					619:629	40% concentrate	615:629	40% concentrate	615:629	A total of 12 ruminally cannulated Holstein cows in mid-lactation were randomly fed either a conventional (CON) diet (40% concentrate, dry matter basis) or an HG diet (60% concentrate, dry matter basis) for 4 wk.
35688738	13	147	theme	Correlation	2478:2488	arg1	analysis					2490:2497	Correlation analysis	2478:2497	Correlation analysis	2478:2497	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	12	148	theme	HG	2308:2309	arg1	diet					2311:2314	the HG diet	2304:2314	the HG diet	2304:2314	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	11	149	theme	ZO-1	2264:2267	arg1	downregulation					2138:2151	a downregulation	2136:2151	a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1	2136:2267	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	12	150	theme	mRNA	2342:2345	arg1	expression					2347:2356	the mRNA expression	2338:2356	the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium	2338:2408	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	0	151	theme	diet	22:25	arg1	feeding					27:33	high-grain diet feeding	11:33	high-grain diet feeding	11:33	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	5	152	theme	nonmetric	1094:1102	arg1	plots					1129:1133	nonmetric multidimensional scaling plots	1094:1133	nonmetric multidimensional scaling plots	1094:1133	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	11	153	theme	claudin-4	2249:2257	arg1	downregulation					2138:2151	a downregulation	2136:2151	a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1	2136:2267	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	12	154	from	increase	2326:2333	arg1	expression					2347:2356	the mRNA expression	2338:2356	the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium	2338:2408	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	3	155	theme	small	810:814	arg1	communities					855:865	the small intestinal mucosa-associated bacterial communities	806:865	the small intestinal mucosa-associated bacterial communities	806:865	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	11	156	theme	ZO-1	2192:2195	arg1	expression					2165:2174	the mRNA expression	2156:2174	the mRNA expression of occludin and ZO-1 in the jejunal epithelium	2156:2221	The HG feeding caused a downregulation of the mRNA expression of occludin and ZO-1 in the jejunal epithelium, as well as of claudin-1, claudin-4, and ZO-1 in the ileal epithelium.
35688738	5	157	theme	scaling	1121:1127	arg1	plots					1129:1133	nonmetric multidimensional scaling plots	1094:1133	nonmetric multidimensional scaling plots	1094:1133	The results of nonmetric multidimensional scaling plots showed that an HG diet altered the composition of mucosa-associated bacterial communities in the jejunum and ileum.
35688738	8	158	from	proportions	1655:1665	arg1	jejunal					1697:1703	jejunal	1697:1703	jejunal	1697:1703	Compared with the CON group, the proportions of Acetitomaculum in both the jejunal and the ileal mucosa were higher in the HG group.
35688738	8	158	from	proportions	1655:1665	arg1	mucosa					1719:1724	the ileal mucosa	1709:1724	the ileal mucosa	1709:1724	Compared with the CON group, the proportions of Acetitomaculum in both the jejunal and the ileal mucosa were higher in the HG group.
35688738	0	159	theme	bacterial	56:64	arg1	community					66:74	mucosa-associated bacterial community	38:74	mucosa-associated bacterial community	38:74	Effects of high-grain diet feeding on mucosa-associated bacterial community and gene expression of tight junction proteins and inflammatory cytokines in the small intestine of dairy cattle.
35688738	7	160	from	abundance	1431:1439	arg1	mucosa					1526:1531	the duodenal mucosa	1513:1531	the duodenal mucosa	1513:1531	At the genus level, the HG diet increased the abundance of uncultured Succinivibrionaceae and Lachnospiraceae incertae sedis in the duodenal mucosa, whereas the proportions of Veillonella and Selenomonas increased in the jejunal mucosa.
35688738	9	161	from	disease	1963:1969	arg1	mucosa					1984:1989	the ileal mucosa	1974:1989	the ileal mucosa	1974:1989	Analysis via PICRUSt2 (version 2.2.0-b) suggested that the HG diet increased the abundance of genes related to biodegradation of xenobiotics in the jejunal mucosa and the abundance of genes related to immune disease in the ileal mucosa.
35688738	6	162	theme	HG	1255:1256	arg1	feeding					1258:1264	The HG feeding	1251:1264	The HG feeding	1251:1264	The HG feeding only increased the numbers of operational taxonomic units in the mucosa-associated bacterial community in the jejunum.
35688738	3	163	theme	mucosa-associated	827:843	arg1	communities					855:865	the small intestinal mucosa-associated bacterial communities	806:865	the small intestinal mucosa-associated bacterial communities	806:865	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
35688738	13	164	from	changes	2626:2632	arg1	expression					2641:2650	the expression	2637:2650	the expression of the epithelial cytokines in the jejunum and ileum	2637:2703	Correlation analysis revealed that the alteration of lipopolysaccharide levels and mucosa-associated bacterial community might partly contribute to changes in the expression of the epithelial cytokines in the jejunum and ileum during HG feeding.
35688738	4	165	theme	inflammatory	974:985	arg1	cytokines					987:995	inflammatory cytokines	974:995	inflammatory cytokines	974:995	Furthermore, the gene expression of tight junction proteins and inflammatory cytokines in the small intestinal epithelium were studied using real-time quantitative PCR.
35688738	12	166	theme	IL-2	2438:2441	arg1	expression					2424:2433	a higher expression	2415:2433	a higher expression of IL-2 and IFN-γ in the ileal epithelium	2415:2475	Moreover, the HG diet caused an increase in the mRNA expression of IL-1β, IL-2, and IFN-γ in the jejunal epithelium, but a higher expression of IL-2 and IFN-γ in the ileal epithelium.
35688738	3	167	theme	amplicon	889:896	arg1	sequencing					898:907	16S full-length amplicon sequencing	873:907	16S full-length amplicon sequencing	873:907	At the end of the feeding trial, all the cows were slaughtered and then examined for changes in the small intestinal mucosa-associated bacterial communities using 16S full-length amplicon sequencing.
36464700	0	0	theme	host	73:76	arg1	diet					78:81	the host diet	69:81	the host diet	69:81	The microbiome's fiber degradation profile and its relationship with the host diet.
36464700	5	1	theme	DFs	1058:1060	arg1	catalog					1047:1053	a broad catalog	1039:1053	a broad catalog of DFs	1039:1060	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	6	2	theme	independent	1224:1234	arg1	datasets					1236:1243	four independent datasets	1219:1243	four independent datasets	1219:1243	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	3	3	theme	fibers	446:451	arg1	Consumption					423:433	Consumption	423:433	Consumption of dietary fibers (DFs)	423:457	Consumption of dietary fibers (DFs)-polysaccharides that cannot be broken down by the host's endogenous enzymes and are degraded primarily by members of the microbiome-is known to have a profound effect on the microbiome.
36464700	8	4	theme	DF	1576:1577	arg1	habits					1591:1596	DF consumption habits	1576:1596	DF consumption habits	1576:1596	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	8	5	theme	metagenomes	1441:1451	arg1	set					1429:1431	a set	1427:1431	a set of ~700 metagenomes that represents large human population cohorts from 9 different countries	1427:1525	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	8	5	theme	metagenomes	1441:1451	arg1	metagenomes					1441:1451	~700 metagenomes	1436:1451	~700 metagenomes	1436:1451	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	8	6	from	countries	1517:1525	arg1	cohorts					1492:1498	large human population cohorts	1469:1498	large human population cohorts from 9 different countries	1469:1525	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	9	7	theme	analysis	1716:1723	arg1	use					1697:1699	the use	1693:1699	the use of DF-specific analysis for providing important complementary information for better understanding the relationship between dietary habits and the gut microbiome	1693:1861	CONCLUSIONS Combined, our findings serve as a proof-of-concept for the use of DF-specific analysis for providing important complementary information for better understanding the relationship between dietary habits and the gut microbiome.
36464700	7	8	dep	taxonomic	1340:1348	arg1	microbiome-based					1365:1380	microbiome-based	1365:1380	microbiome-based	1365:1380	We further demonstrate that IFDPs are more tightly linked to the host diet than commonly used taxonomic and functional microbiome-based profiles.
36464700	5	9	theme	degrading	926:934	arg1	enzymes					936:942	polysaccharide degrading enzymes	911:942	polysaccharide degrading enzymes	911:942	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	3	10	theme	profound	610:617	arg1	effect					619:624	a profound effect	608:624	a profound effect	608:624	Consumption of dietary fibers (DFs)-polysaccharides that cannot be broken down by the host's endogenous enzymes and are degraded primarily by members of the microbiome-is known to have a profound effect on the microbiome.
36464700	6	11	theme	hosts	1206:1210	arg1	habits					1188:1193	the dietary habits	1176:1193	the dietary habits of various hosts across four independent datasets	1176:1243	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	6	12	theme	fiber	1096:1100	arg1	IFDP					1123:1126	IFDP	1123:1126	IFDP	1123:1126	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	6	12	theme	fiber	1096:1100	arg1	profile					1114:1120	the inferred fiber degradation profile	1083:1120	the inferred fiber degradation profile (IFDP) generated by our framework	1083:1154	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	4	13	theme	microbiome	686:695	arg1	shifts					726:731	microbiome compositional and functional shifts	686:731	microbiome compositional and functional shifts in response to the consumption of specific DFs	686:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	5	14	theme	careful	889:895	arg1	annotation					897:906	careful annotation	889:906	careful annotation of polysaccharide degrading enzymes and DF structures	889:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	6	15	theme	inferred	1087:1094	arg1	IFDP					1123:1126	IFDP	1123:1126	IFDP	1123:1126	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	6	15	theme	inferred	1087:1094	arg1	profile					1114:1120	the inferred fiber degradation profile	1083:1120	the inferred fiber degradation profile (IFDP) generated by our framework	1083:1154	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	6	16	theme	various	1198:1204	arg1	hosts					1206:1210	various hosts	1198:1210	various hosts	1198:1210	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	7	17	theme	functional	1354:1363	arg1	profiles					1382:1389	commonly used taxonomic and functional microbiome-based profiles	1326:1389	commonly used taxonomic and functional microbiome-based profiles	1326:1389	We further demonstrate that IFDPs are more tightly linked to the host diet than commonly used taxonomic and functional microbiome-based profiles.
36464700	5	18	theme	coupling	852:859	arg1	framework					841:849	a computational framework	825:849	a computational framework	825:849	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	5	18	theme	coupling	852:859	arg1	sequencing					873:882	coupling metagenomic sequencing	852:882	coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures	852:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	9	19	theme	complementary	1749:1761	arg1	information					1763:1773	important complementary information	1739:1773	important complementary information for better understanding the relationship between dietary habits and the gut microbiome	1739:1861	CONCLUSIONS Combined, our findings serve as a proof-of-concept for the use of DF-specific analysis for providing important complementary information for better understanding the relationship between dietary habits and the gut microbiome.
36464700	4	20	theme	DFs	776:778	arg1	consumption					752:762	the consumption	748:762	the consumption of specific DFs	748:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	0	21	with	relationship	51:62	arg1	diet					78:81	the host diet	69:81	the host diet	69:81	The microbiome's fiber degradation profile and its relationship with the host diet.
36464700	5	22	theme	polysaccharide	911:924	arg1	enzymes					936:942	polysaccharide degrading enzymes	911:942	polysaccharide degrading enzymes	911:942	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	8	23	theme	consumption	1579:1589	arg1	habits					1591:1596	DF consumption habits	1576:1596	DF consumption habits	1576:1596	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	5	24	theme	broad	1041:1045	arg1	catalog					1047:1053	a broad catalog	1039:1053	a broad catalog of DFs	1039:1060	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	4	25	theme	specific	767:774	arg1	DFs					776:778	specific DFs	767:778	specific DFs	767:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	4	26	from	characterization	666:681	arg1	response					736:743	response	736:743	response to the consumption of specific DFs	736:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	2	27	theme	diet	242:245	arg1	ability					313:319	the microbiome's ability	296:319	the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host	296:420	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	2	27	theme	diet	242:245	arg1	impact					232:237	the impact	228:237	the impact of diet on the composition of the microbiome	228:282	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	7	28	theme	used	1335:1338	arg1	profiles					1382:1389	commonly used taxonomic and functional microbiome-based profiles	1326:1389	commonly used taxonomic and functional microbiome-based profiles	1326:1389	We further demonstrate that IFDPs are more tightly linked to the host diet than commonly used taxonomic and functional microbiome-based profiles.
36464700	7	29	theme	host	1311:1314	arg1	diet					1316:1319	the host diet	1307:1319	the host diet than commonly used taxonomic and functional microbiome-based profiles	1307:1389	We further demonstrate that IFDPs are more tightly linked to the host diet than commonly used taxonomic and functional microbiome-based profiles.
36464700	8	30	theme	large	1469:1473	arg1	cohorts					1492:1498	large human population cohorts	1469:1498	large human population cohorts from 9 different countries	1469:1525	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	6	31	theme	dietary	1180:1186	arg1	habits					1188:1193	the dietary habits	1176:1193	the dietary habits of various hosts across four independent datasets	1176:1243	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	5	32	theme	metagenomic	861:871	arg1	framework					841:849	a computational framework	825:849	a computational framework	825:849	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	5	32	theme	metagenomic	861:871	arg1	sequencing					873:882	coupling metagenomic sequencing	852:882	coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures	852:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	2	33	theme	various	332:338	arg1	compounds					340:348	various compounds	332:348	various compounds in the diet	332:360	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	2	34	theme	microbiome	273:282	arg1	composition					254:264	the composition	250:264	the composition of the microbiome	250:282	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	0	35	theme	fiber	17:21	arg1	profile					35:41	The microbiome's fiber degradation profile	0:41	The microbiome's fiber degradation profile	0:41	The microbiome's fiber degradation profile and its relationship with the host diet.
36464700	7	36	theme	taxonomic	1340:1348	arg1	profiles					1382:1389	commonly used taxonomic and functional microbiome-based profiles	1326:1389	commonly used taxonomic and functional microbiome-based profiles	1326:1389	We further demonstrate that IFDPs are more tightly linked to the host diet than commonly used taxonomic and functional microbiome-based profiles.
36464700	9	37	theme	important	1739:1747	arg1	information					1763:1773	important complementary information	1739:1773	important complementary information for better understanding the relationship between dietary habits and the gut microbiome	1739:1861	CONCLUSIONS Combined, our findings serve as a proof-of-concept for the use of DF-specific analysis for providing important complementary information for better understanding the relationship between dietary habits and the gut microbiome.
36464700	2	38	from	ability	313:319	arg1	composition					254:264	the composition	250:264	the composition of the microbiome	250:282	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	5	39	dep	RESULTS	798:804	arg1	introduce					815:823	introduce	815:823	introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs	815:1060	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	1	40	theme	numerous	170:177	arg1	studies					186:192	numerous recent studies	170:192	numerous recent studies	170:192	BACKGROUND The relationship between the gut microbiome and diet has been the focus of numerous recent studies.
36464700	5	41	with	sequencing	873:882	arg1	annotation					897:906	careful annotation	889:906	careful annotation of polysaccharide degrading enzymes and DF structures	889:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	6	42	theme	degradation	1102:1112	arg1	IFDP					1123:1126	IFDP	1123:1126	IFDP	1123:1126	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	6	42	theme	degradation	1102:1112	arg1	profile					1114:1120	the inferred fiber degradation profile	1083:1120	the inferred fiber degradation profile (IFDP) generated by our framework	1083:1154	We demonstrate that the inferred fiber degradation profile (IFDP) generated by our framework accurately reflects the dietary habits of various hosts across four independent datasets.
36464700	5	43	theme	given	1004:1008	arg1	sample					1021:1026	a given microbiome sample	1002:1026	a given microbiome sample	1002:1026	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	4	44	theme	comprehensive	652:664	arg1	characterization					666:681	a comprehensive characterization	650:681	a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs	650:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	1	45	theme	recent	179:184	arg1	studies					186:192	numerous recent studies	170:192	numerous recent studies	170:192	BACKGROUND The relationship between the gut microbiome and diet has been the focus of numerous recent studies.
36464700	5	46	theme	microbiome	1010:1019	arg1	sample					1021:1026	a given microbiome sample	1002:1026	a given microbiome sample	1002:1026	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	3	47	theme	endogenous	516:525	arg1	enzymes					527:533	the host's endogenous enzymes	505:533	the host's endogenous enzymes	505:533	Consumption of dietary fibers (DFs)-polysaccharides that cannot be broken down by the host's endogenous enzymes and are degraded primarily by members of the microbiome-is known to have a profound effect on the microbiome.
36464700	2	48	from	impact	232:237	arg1	composition					254:264	the composition	250:264	the composition of the microbiome	250:282	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	1	49	theme	studies	186:192	arg1	focus					161:165	the focus	157:165	the focus of numerous recent studies	157:192	BACKGROUND The relationship between the gut microbiome and diet has been the focus of numerous recent studies.
36464700	1	49	theme	studies	186:192	arg1	relationship					99:110	The relationship	95:110	The relationship between the gut microbiome and diet	95:146	BACKGROUND The relationship between the gut microbiome and diet has been the focus of numerous recent studies.
36464700	0	50	theme	degradation	23:33	arg1	profile					35:41	The microbiome's fiber degradation profile	0:41	The microbiome's fiber degradation profile	0:41	The microbiome's fiber degradation profile and its relationship with the host diet.
36464700	5	51	theme	sample	1021:1026	arg1	ability					991:997	the metabolic ability	977:997	the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs	977:1060	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	9	52	theme	dietary	1825:1831	arg1	habits					1833:1838	dietary habits	1825:1838	dietary habits	1825:1838	CONCLUSIONS Combined, our findings serve as a proof-of-concept for the use of DF-specific analysis for providing important complementary information for better understanding the relationship between dietary habits and the gut microbiome.
36464700	8	53	theme	different	1507:1515	arg1	countries					1517:1525	9 different countries	1505:1525	9 different countries	1505:1525	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	8	54	theme	population	1481:1490	arg1	cohorts					1492:1498	large human population cohorts	1469:1498	large human population cohorts from 9 different countries	1469:1525	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	8	55	theme	human	1475:1479	arg1	cohorts					1492:1498	large human population cohorts	1469:1498	large human population cohorts from 9 different countries	1469:1525	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	5	56	theme	computational	827:839	arg1	framework					841:849	a computational framework	825:849	a computational framework	825:849	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	5	56	theme	computational	827:839	arg1	sequencing					873:882	coupling metagenomic sequencing	852:882	coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures	852:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	8	57	theme	~700	1436:1439	arg1	metagenomes					1441:1451	~700 metagenomes	1436:1451	~700 metagenomes	1436:1451	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	5	58	theme	metabolic	981:989	arg1	ability					991:997	the metabolic ability	977:997	the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs	977:1060	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	2	59	theme	Such	195:198	arg1	studies					200:206	Such studies	195:206	Such studies	195:206	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	3	60	theme	dietary	438:444	arg1	DFs					454:456	DFs	454:456	DFs	454:456	Consumption of dietary fibers (DFs)-polysaccharides that cannot be broken down by the host's endogenous enzymes and are degraded primarily by members of the microbiome-is known to have a profound effect on the microbiome.
36464700	3	60	theme	dietary	438:444	arg1	fibers					446:451	dietary fibers	438:451	dietary fibers (DFs)	438:457	Consumption of dietary fibers (DFs)-polysaccharides that cannot be broken down by the host's endogenous enzymes and are degraded primarily by members of the microbiome-is known to have a profound effect on the microbiome.
36464700	8	61	theme	microbiome	1603:1612	arg1	capacities					1614:1623	microbiome capacities	1603:1623	microbiome capacities	1603:1623	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	5	62	theme	enzymes	936:942	arg1	annotation					897:906	careful annotation	889:906	careful annotation of polysaccharide degrading enzymes and DF structures	889:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	4	63	theme	shifts	726:731	arg1	characterization					666:681	a comprehensive characterization	650:681	a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs	650:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	2	64	from	compounds	340:348	arg1	diet					357:360	the diet	353:360	the diet	353:360	Such studies aim to characterize the impact of diet on the composition of the microbiome, as well as the microbiome's ability to utilize various compounds in the diet and produce metabolites that may be beneficial for the host.
36464700	4	65	from	shifts	726:731	arg1	response					736:743	response	736:743	response to the consumption of specific DFs	736:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	7	66	attach	linked	1297:1302	arg2	IFDPs					1274:1278	IFDPs	1274:1278	IFDPs	1274:1278	We further demonstrate that IFDPs are more tightly linked to the host diet than commonly used taxonomic and functional microbiome-based profiles.
36464700	7	66	attach	linked	1297:1302	arg1	diet					1316:1319	the host diet	1307:1319	the host diet than commonly used taxonomic and functional microbiome-based profiles	1307:1389	We further demonstrate that IFDPs are more tightly linked to the host diet than commonly used taxonomic and functional microbiome-based profiles.
36464700	4	67	theme	compositional	697:709	arg1	shifts					726:731	microbiome compositional and functional shifts	686:731	microbiome compositional and functional shifts in response to the consumption of specific DFs	686:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	3	68	theme	microbiome-is	580:592	arg1	members					565:571	members	565:571	members of the microbiome-is known to have a profound effect on the microbiome	565:642	Consumption of dietary fibers (DFs)-polysaccharides that cannot be broken down by the host's endogenous enzymes and are degraded primarily by members of the microbiome-is known to have a profound effect on the microbiome.
36464700	4	69	theme	functional	715:724	arg1	shifts					726:731	microbiome compositional and functional shifts	686:731	microbiome compositional and functional shifts in response to the consumption of specific DFs	686:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	4	70	from	response	736:743	arg1	characterization					666:681	a comprehensive characterization	650:681	a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs	650:778	Yet, a comprehensive characterization of microbiome compositional and functional shifts in response to the consumption of specific DFs is still lacking.
36464700	1	71	theme	gut	124:126	arg1	microbiome					128:137	the gut microbiome	120:137	the gut microbiome	120:137	BACKGROUND The relationship between the gut microbiome and diet has been the focus of numerous recent studies.
36464700	9	72	theme	gut	1848:1850	arg1	microbiome					1852:1861	the gut microbiome	1844:1861	the gut microbiome	1844:1861	CONCLUSIONS Combined, our findings serve as a proof-of-concept for the use of DF-specific analysis for providing important complementary information for better understanding the relationship between dietary habits and the gut microbiome.
36464700	5	73	theme	DF	948:949	arg1	structures					951:960	DF structures	948:960	DF structures	948:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	1	74	dep	BACKGROUND	84:93	arg1	focus					161:165	the focus	157:165	the focus of numerous recent studies	157:192	BACKGROUND The relationship between the gut microbiome and diet has been the focus of numerous recent studies.
36464700	1	74	dep	BACKGROUND	84:93	arg1	relationship					99:110	The relationship	95:110	The relationship between the gut microbiome and diet	95:146	BACKGROUND The relationship between the gut microbiome and diet has been the focus of numerous recent studies.
36464700	9	75	theme	DF-specific	1704:1714	arg1	analysis					1716:1723	DF-specific analysis	1704:1723	DF-specific analysis	1704:1723	CONCLUSIONS Combined, our findings serve as a proof-of-concept for the use of DF-specific analysis for providing important complementary information for better understanding the relationship between dietary habits and the gut microbiome.
36464700	5	76	theme	structures	951:960	arg1	annotation					897:906	careful annotation	889:906	careful annotation of polysaccharide degrading enzymes and DF structures	889:960	RESULTS Here, we introduce a computational framework, coupling metagenomic sequencing with careful annotation of polysaccharide degrading enzymes and DF structures, for inferring the metabolic ability of a given microbiome sample to utilize a broad catalog of DFs.
36464700	0	77	with	profile	35:41	arg1	diet					78:81	the host diet	69:81	the host diet	69:81	The microbiome's fiber degradation profile and its relationship with the host diet.
36464700	8	78	theme	intriguing	1541:1550	arg1	patterns					1559:1566	intriguing global patterns	1541:1566	intriguing global patterns linking DF consumption habits with microbiome capacities	1541:1623	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36464700	8	79	theme	global	1552:1557	arg1	patterns					1559:1566	intriguing global patterns	1541:1566	intriguing global patterns linking DF consumption habits with microbiome capacities	1541:1623	Finally, applying our framework to a set of ~700 metagenomes that represents large human population cohorts from 9 different countries, we highlight intriguing global patterns linking DF consumption habits with microbiome capacities.
36085403	4	0	theme	microbial	606:614	arg1	diversity					616:624	microbial diversity	606:624	microbial diversity	606:624	Fecal samples from pigs were analyzed for microbial diversity, composition, and predicted functionality using 16S rRNA gene sequencing.
36085403	8	1	theme	fat	1385:1387	arg1	synthesis					1389:1397	fat synthesis	1385:1397	fat synthesis	1385:1397	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	1	2	theme	deposition	133:142	arg1	capacity					144:151	fat deposition capacity	129:151	fat deposition capacity	129:151	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	4	3	theme	gene	683:686	arg1	sequencing					688:697	16S rRNA gene sequencing	674:697	16S rRNA gene sequencing	674:697	Fecal samples from pigs were analyzed for microbial diversity, composition, and predicted functionality using 16S rRNA gene sequencing.
36085403	1	4	theme	capacity	144:151	arg1	indicator					116:124	an important indicator	103:124	an important indicator of fat deposition capacity and lean meat rate	103:170	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	1	4	theme	capacity	144:151	arg1	thickness					85:93	Backfat thickness	77:93	Backfat thickness (BF)	77:98	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	10	5	theme	targets	1753:1759	arg1	investigation					1736:1748	the investigation	1732:1748	the investigation of targets for dietary strategies	1732:1782	The results of this study will be useful for the development of microbial biomarkers for predicting and improving porcine BF, as well as for the investigation of targets for dietary strategies.
36085403	0	6	from	Correlation	0:10	arg1	pigs					71:74	crossbred pigs	61:74	crossbred pigs	61:74	Correlation between fat accumulation and fecal microbiota in crossbred pigs.
36085403	10	7	theme	microbial	1655:1663	arg1	biomarkers					1665:1674	microbial biomarkers	1655:1674	microbial biomarkers for predicting and improving porcine BF	1655:1714	The results of this study will be useful for the development of microbial biomarkers for predicting and improving porcine BF, as well as for the investigation of targets for dietary strategies.
36085403	8	8	dep	BF	1247:1248	arg1	belong					1257:1262	belong	1257:1262	BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition	1247:1412	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	4	9	theme	rRNA	678:681	arg1	sequencing					688:697	16S rRNA gene sequencing	674:697	16S rRNA gene sequencing	674:697	Fecal samples from pigs were analyzed for microbial diversity, composition, and predicted functionality using 16S rRNA gene sequencing.
36085403	8	10	theme	host-indigestible	1298:1314	arg1	polysaccharides					1322:1336	host-indigestible plant polysaccharides	1298:1336	host-indigestible plant polysaccharides into short-chain fatty acid (SCFA)	1298:1371	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	6	11	theme	H-BF	911:914	arg1	pigs					916:919	H-BF pigs	911:919	H-BF pigs	911:919	LEfSe analysis revealed a number of bacterial features being differentially enriched in either L-BF or H-BF pigs.
36085403	4	12	theme	predicted	644:652	arg1	functionality					654:666	predicted functionality	644:666	predicted functionality	644:666	Fecal samples from pigs were analyzed for microbial diversity, composition, and predicted functionality using 16S rRNA gene sequencing.
36085403	4	13	theme	16S	674:676	arg1	sequencing					688:697	16S rRNA gene sequencing	674:697	16S rRNA gene sequencing	674:697	Fecal samples from pigs were analyzed for microbial diversity, composition, and predicted functionality using 16S rRNA gene sequencing.
36085403	2	14	theme	profound	320:327	arg1	impact					329:334	a profound impact	318:334	a profound impact on fat deposition of livestock animals	318:373	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	4	15	from	pigs	583:586	arg1	samples					570:576	Fecal samples	564:576	Fecal samples from pigs	564:586	Fecal samples from pigs were analyzed for microbial diversity, composition, and predicted functionality using 16S rRNA gene sequencing.
36085403	6	16	theme	LEfSe	808:812	arg1	analysis					814:821	LEfSe analysis	808:821	LEfSe analysis	808:821	LEfSe analysis revealed a number of bacterial features being differentially enriched in either L-BF or H-BF pigs.
36085403	2	17	theme	livestock	357:365	arg1	animals					367:373	livestock animals	357:373	livestock animals	357:373	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	2	18	theme	Intestinal	236:245	arg1	microbiota					247:256	Intestinal microbiota	236:256	Intestinal microbiota	236:256	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	1	19	theme	Backfat	77:83	arg1	indicator					116:124	an important indicator	103:124	an important indicator of fat deposition capacity and lean meat rate	103:170	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	1	19	theme	Backfat	77:83	arg1	BF					96:97	BF	96:97	BF	96:97	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	1	19	theme	Backfat	77:83	arg1	thickness					85:93	Backfat thickness	77:93	Backfat thickness (BF)	77:98	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	3	20	from	pigs	537:540	arg1	groups					512:517	two groups	508:517	two groups from Yunong Black pigs in the present study	508:561	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	3	20	from	pigs	537:540	arg1	study					557:561	the present study	545:561	the present study	545:561	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	3	20	from	pigs	537:540	arg1	pigs					486:489	20 low-BF (L-BF) and 20 high-BF (H-BF) pigs	447:489	20 low-BF (L-BF) and 20 high-BF (H-BF) pigs	447:489	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	1	21	theme	lean	157:160	arg1	rate					167:170	lean meat rate	157:170	lean meat rate	157:170	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	3	22	theme	high-BF	471:477	arg1	groups					512:517	two groups	508:517	two groups from Yunong Black pigs in the present study	508:561	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	3	22	theme	high-BF	471:477	arg1	pigs					486:489	20 low-BF (L-BF) and 20 high-BF (H-BF) pigs	447:489	20 low-BF (L-BF) and 20 high-BF (H-BF) pigs	447:489	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	1	23	from	indicator	116:124	arg1	pigs					175:178	pigs	175:178	pigs	175:178	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	2	24	theme	nutrient	278:285	arg1	digestion					287:295	nutrient digestion	278:295	nutrient digestion	278:295	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	1	25	theme	meat	162:165	arg1	rate					167:170	lean meat rate	157:170	lean meat rate	157:170	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	9	26	theme	H-BF	1579:1582	arg1	group					1584:1588	the H-BF group	1575:1588	the H-BF group	1575:1588	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	9	27	from	microbiota	1561:1570	arg1	abundance					1473:1481	the pathway abundance	1461:1481	the pathway abundance of cell motility and glycan biosynthesis	1461:1522	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	9	27	from	microbiota	1561:1570	arg1	widespread					1543:1552	widespread	1543:1552	widespread	1543:1552	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	2	28	theme	fat	339:341	arg1	deposition					343:352	fat deposition	339:352	fat deposition of livestock animals	339:373	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	5	29	theme	microbial	762:770	arg1	diversity					774:782	microbial β diversity	762:782	microbial β diversity	762:782	The results showed that there were significant differences in microbial β diversity between the two groups.
36085403	9	30	theme	group	1584:1588	arg1	microbiota					1561:1570	the microbiota	1557:1570	the microbiota of the H-BF group	1557:1588	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	1	31	from	breeding	226:233	arg1	important					192:200	important	192:200	important	192:200	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	2	32	from	impact	329:334	arg1	deposition					343:352	fat deposition	339:352	fat deposition of livestock animals	339:373	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	8	33	theme	plant	1316:1320	arg1	polysaccharides					1322:1336	host-indigestible plant polysaccharides	1298:1336	host-indigestible plant polysaccharides into short-chain fatty acid (SCFA)	1298:1371	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	10	34	theme	study	1611:1615	arg1	results					1595:1601	The results	1591:1601	The results of this study	1591:1615	The results of this study will be useful for the development of microbial biomarkers for predicting and improving porcine BF, as well as for the investigation of targets for dietary strategies.
36085403	10	34	theme	study	1611:1615	arg1	useful					1625:1630	useful	1625:1630	useful	1625:1630	The results of this study will be useful for the development of microbial biomarkers for predicting and improving porcine BF, as well as for the investigation of targets for dietary strategies.
36085403	0	35	theme	fat	20:22	arg1	accumulation					24:35	fat accumulation	20:35	fat accumulation	20:35	Correlation between fat accumulation and fecal microbiota in crossbred pigs.
36085403	7	36	theme	Spearman	922:929	arg1	analysis					943:950	Spearman correlation analysis	922:950	Spearman correlation analysis	922:950	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	2	37	theme	key	266:268	arg1	role					270:273	a key role	264:273	a key role	264:273	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	10	38	theme	porcine	1705:1711	arg1	BF					1713:1714	porcine BF	1705:1714	porcine BF	1705:1714	The results of this study will be useful for the development of microbial biomarkers for predicting and improving porcine BF, as well as for the investigation of targets for dietary strategies.
36085403	0	39	theme	fecal	41:45	arg1	microbiota					47:56	fecal microbiota	41:56	fecal microbiota	41:56	Correlation between fat accumulation and fecal microbiota in crossbred pigs.
36085403	9	40	theme	biosynthesis	1511:1522	arg1	abundance					1473:1481	the pathway abundance	1461:1481	the pathway abundance of cell motility and glycan biosynthesis	1461:1522	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	9	40	theme	biosynthesis	1511:1522	arg1	widespread					1543:1552	widespread	1543:1552	widespread	1543:1552	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	1	41	from	genetics	213:220	arg1	important					192:200	important	192:200	important	192:200	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	2	42	with	digestion	287:295	arg1	impact					329:334	a profound impact	318:334	a profound impact on fat deposition of livestock animals	318:373	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	1	43	theme	rate	167:170	arg1	indicator					116:124	an important indicator	103:124	an important indicator of fat deposition capacity and lean meat rate	103:170	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	1	43	theme	rate	167:170	arg1	thickness					85:93	Backfat thickness	77:93	Backfat thickness (BF)	77:98	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	3	44	theme	pig	420:422	arg1	microbiome					428:437	the pig gut microbiome	416:437	the pig gut microbiome	416:437	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	8	45	theme	short-chain	1343:1353	arg1	SCFA					1367:1370	SCFA	1367:1370	SCFA	1367:1370	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	8	45	theme	short-chain	1343:1353	arg1	acid					1361:1364	short-chain fatty acid	1343:1364	short-chain fatty acid (SCFA)	1343:1371	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	5	46	theme	β	772:772	arg1	diversity					774:782	microbial β diversity	762:782	microbial β diversity	762:782	The results showed that there were significant differences in microbial β diversity between the two groups.
36085403	2	47	theme	animals	367:373	arg1	deposition					343:352	fat deposition	339:352	fat deposition of livestock animals	339:373	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	7	48	theme	P	1071:1071	arg1	BF					1067:1068	BF	1067:1068	BF (P < 0.05)	1067:1079	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	7	48	theme	P	1071:1071	arg1	<					1073:1073	P < 0.05	1071:1078	P < 0.05	1071:1078	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	10	49	theme	dietary	1765:1771	arg1	strategies					1773:1782	dietary strategies	1765:1782	dietary strategies	1765:1782	The results of this study will be useful for the development of microbial biomarkers for predicting and improving porcine BF, as well as for the investigation of targets for dietary strategies.
36085403	2	50	with	utilization	301:311	arg1	impact					329:334	a profound impact	318:334	a profound impact on fat deposition of livestock animals	318:373	Intestinal microbiota plays a key role in nutrient digestion and utilization with a profound impact on fat deposition of livestock animals.
36085403	8	51	theme	fatty	1355:1359	arg1	SCFA					1367:1370	SCFA	1367:1370	SCFA	1367:1370	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	8	51	theme	fatty	1355:1359	arg1	acid					1361:1364	short-chain fatty acid	1343:1364	short-chain fatty acid (SCFA)	1343:1371	Importantly, the bacteria significantly positively correlated with BF mainly belong to Clostridium, which can ferment host-indigestible plant polysaccharides into short-chain fatty acid (SCFA) and promote fat synthesis and deposition.
36085403	9	52	theme	functional	1426:1435	arg1	analysis					1437:1444	Predictive functional analysis	1415:1444	Predictive functional analysis	1415:1444	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	6	53	theme	features	854:861	arg1	number					834:839	a number	832:839	a number of bacterial features	832:861	LEfSe analysis revealed a number of bacterial features being differentially enriched in either L-BF or H-BF pigs.
36085403	3	54	theme	present	549:555	arg1	study					557:561	the present study	545:561	the present study	545:561	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	7	55	theme	positive	1040:1047	arg1	correlations					1049:1060	positive correlations	1040:1060	positive correlations with BF (P < 0.05)	1040:1079	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	5	56	from	differences	747:757	arg1	diversity					774:782	microbial β diversity	762:782	microbial β diversity	762:782	The results showed that there were significant differences in microbial β diversity between the two groups.
36085403	3	57	theme	low-BF	450:455	arg1	groups					512:517	two groups	508:517	two groups from Yunong Black pigs in the present study	508:561	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	3	57	theme	low-BF	450:455	arg1	pigs					486:489	20 low-BF (L-BF) and 20 high-BF (H-BF) pigs	447:489	20 low-BF (L-BF) and 20 high-BF (H-BF) pigs	447:489	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	6	58	theme	bacterial	844:852	arg1	features					854:861	bacterial features	844:861	bacterial features	844:861	LEfSe analysis revealed a number of bacterial features being differentially enriched in either L-BF or H-BF pigs.
36085403	9	59	theme	glycan	1504:1509	arg1	biosynthesis					1511:1522	glycan biosynthesis	1504:1522	glycan biosynthesis	1504:1522	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	7	60	theme	correlation	931:941	arg1	analysis					943:950	Spearman correlation analysis	922:950	Spearman correlation analysis	922:950	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	1	61	theme	important	106:114	arg1	indicator					116:124	an important indicator	103:124	an important indicator of fat deposition capacity and lean meat rate	103:170	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	1	61	theme	important	106:114	arg1	thickness					85:93	Backfat thickness	77:93	Backfat thickness (BF)	77:98	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	9	62	from	widespread	1543:1552	arg1	microbiota					1561:1570	the microbiota	1557:1570	the microbiota of the H-BF group	1557:1588	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	10	63	theme	biomarkers	1665:1674	arg1	development					1640:1650	the development	1636:1650	the development of microbial biomarkers for predicting and improving porcine BF	1636:1714	The results of this study will be useful for the development of microbial biomarkers for predicting and improving porcine BF, as well as for the investigation of targets for dietary strategies.
36085403	1	64	from	important	192:200	arg1	breeding					226:233	breeding	226:233	breeding	226:233	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	1	64	from	important	192:200	arg1	genetics					213:220	porcine genetics	205:220	porcine genetics	205:220	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	9	65	theme	cell	1486:1489	arg1	motility					1491:1498	cell motility	1486:1498	cell motility	1486:1498	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	5	66	theme	significant	735:745	arg1	differences					747:757	significant differences	735:757	significant differences in microbial β diversity between the two groups	735:805	The results showed that there were significant differences in microbial β diversity between the two groups.
36085403	9	67	theme	pathway	1465:1471	arg1	abundance					1473:1481	the pathway abundance	1461:1481	the pathway abundance of cell motility and glycan biosynthesis	1461:1522	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	9	67	theme	pathway	1465:1471	arg1	widespread					1543:1552	widespread	1543:1552	widespread	1543:1552	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	9	68	theme	Predictive	1415:1424	arg1	analysis					1437:1444	Predictive functional analysis	1415:1444	Predictive functional analysis	1415:1444	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	3	69	theme	gut	424:426	arg1	microbiome					428:437	the pig gut microbiome	416:437	the pig gut microbiome	416:437	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	3	70	theme	Yunong	524:529	arg1	pigs					537:540	Yunong Black pigs	524:540	Yunong Black pigs in the present study	524:561	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	7	71	with	correlations	1049:1060	arg1	BF					1067:1068	BF	1067:1068	BF (P < 0.05)	1067:1079	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	7	71	with	correlations	1049:1060	arg1	<					1073:1073	P < 0.05	1071:1078	P < 0.05	1071:1078	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	4	72	theme	Fecal	564:568	arg1	samples					570:576	Fecal samples	564:576	Fecal samples from pigs	564:586	Fecal samples from pigs were analyzed for microbial diversity, composition, and predicted functionality using 16S rRNA gene sequencing.
36085403	9	73	theme	motility	1491:1498	arg1	abundance					1473:1481	the pathway abundance	1461:1481	the pathway abundance of cell motility and glycan biosynthesis	1461:1522	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	9	73	theme	motility	1491:1498	arg1	widespread					1543:1552	widespread	1543:1552	widespread	1543:1552	Predictive functional analysis indicated that the pathway abundance of cell motility and glycan biosynthesis were significantly widespread in the microbiota of the H-BF group.
36085403	0	74	theme	crossbred	61:69	arg1	pigs					71:74	crossbred pigs	61:74	crossbred pigs	61:74	Correlation between fat accumulation and fecal microbiota in crossbred pigs.
36085403	3	75	theme	Black	531:535	arg1	pigs					537:540	Yunong Black pigs	524:540	Yunong Black pigs in the present study	524:561	To investigate the relationship between the pig gut microbiome and BF, 20 low-BF (L-BF) and 20 high-BF (H-BF) pigs were selected as two groups from Yunong Black pigs in the present study.
36085403	1	76	theme	fat	129:131	arg1	capacity					144:151	fat deposition capacity	129:151	fat deposition capacity	129:151	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
36085403	7	77	theme	P	1169:1169	arg1	BF					1165:1166	BF	1165:1166	BF (P < 0.05)	1165:1177	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	7	77	theme	P	1169:1169	arg1	<					1171:1171	P < 0.05	1169:1176	P < 0.05	1169:1176	Spearman correlation analysis identified the abundance of Oscillospira, Peptococcus, and Bulleidia were significantly positive correlations with BF (P < 0.05), while Sutterella and Bifidobacterium were significantly negatively correlated with BF (P < 0.05).
36085403	1	78	theme	porcine	205:211	arg1	genetics					213:220	porcine genetics	205:220	porcine genetics	205:220	Backfat thickness (BF) is an important indicator of fat deposition capacity and lean meat rate in pigs and is very important in porcine genetics and breeding.
35358641	3	0	theme	Recent	350:355	arg1	studies					357:363	Recent studies	350:363	Recent studies	350:363	Recent studies have reported that probiotics can attenuate anxiety-like behaviors by modulation of the gut microbiome composition.
35358641	4	1	theme	boulardii	549:557	arg1	administration					564:577	Saccharomyces boulardii (Sb) administration	535:577	Saccharomyces boulardii (Sb) administration	535:577	The present study aimed to investigate the effects of Saccharomyces boulardii (Sb) administration on anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats.
35358641	10	2	theme	anxiety-like	1210:1221	arg1	behaviors					1223:1231	LPS-induced anxiety-like behaviors	1198:1231	LPS-induced anxiety-like behaviors	1198:1231	The results revealed that S. boulardii could attenuate LPS-induced anxiety-like behaviors.
35358641	11	3	theme	boulardii	1290:1298	arg1	administration					1269:1282	oral administration	1264:1282	oral administration of S. boulardii	1264:1298	The findings also showed that oral administration of S. boulardii significantly attenuated the elevated levels of cortisol and corticosterone in the LPS-induced model.
35358641	9	4	theme	cortisol	1046:1053	arg1	levels					1036:1041	the serum levels	1026:1041	the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF)	1026:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	10	5	theme	LPS-induced	1198:1208	arg1	behaviors					1223:1231	LPS-induced anxiety-like behaviors	1198:1231	LPS-induced anxiety-like behaviors	1198:1231	The results revealed that S. boulardii could attenuate LPS-induced anxiety-like behaviors.
35358641	9	6	link	brain-derived	1087:1099	arg1	BDNF					1122:1125	BDNF	1122:1125	BDNF	1122:1125	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	9	6	link	brain-derived	1087:1099	arg1	factor					1114:1119	brain-derived neurotrophic factor	1087:1119	brain-derived neurotrophic factor (BDNF)	1087:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	2	7	theme	glucocorticoids	309:323	arg1	secretion					296:304	the secretion	292:304	the secretion of glucocorticoids associated with anxiety	292:347	Exposure to stressors can cause gut microbiota dysbiosis and activate the hypothalamic-pituitary-adrenal (HPA) axis, leading to the secretion of glucocorticoids associated with anxiety.
35358641	11	8	from	levels	1338:1343	arg1	model					1395:1399	the LPS-induced model	1379:1399	the LPS-induced model	1379:1399	The findings also showed that oral administration of S. boulardii significantly attenuated the elevated levels of cortisol and corticosterone in the LPS-induced model.
35358641	9	9	theme	corticosterone	1056:1069	arg1	levels					1036:1041	the serum levels	1026:1041	the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF)	1026:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	12	10	from	effect	1452:1457	arg1	levels					1498:1503	the serum serotonin and BDNF levels	1469:1503	levels	1498:1503	Moreover, S. boulardii alleviated the decremental effect of LPS on the serum serotonin and BDNF levels.
35358641	12	10	from	effect	1452:1457	arg1	serotonin					1479:1487	the serum serotonin and BDNF levels	1469:1503	serotonin	1479:1487	Moreover, S. boulardii alleviated the decremental effect of LPS on the serum serotonin and BDNF levels.
35358641	12	11	theme	decremental	1440:1450	arg1	effect					1452:1457	the decremental effect	1436:1457	the decremental effect of LPS on the serum serotonin and BDNF levels	1436:1503	Moreover, S. boulardii alleviated the decremental effect of LPS on the serum serotonin and BDNF levels.
35358641	8	12	theme	elevated	976:983	arg1	plus-maze					985:993	the elevated plus-maze	972:993	the elevated plus-maze	972:993	Anxiety-like behaviors were assessed using the elevated plus-maze and open-field tests.
35358641	12	13	theme	LPS	1462:1464	arg1	effect					1452:1457	the decremental effect	1436:1457	the decremental effect of LPS on the serum serotonin and BDNF levels	1436:1503	Moreover, S. boulardii alleviated the decremental effect of LPS on the serum serotonin and BDNF levels.
35358641	11	14	theme	elevated	1329:1336	arg1	levels					1338:1343	the elevated levels	1325:1343	the elevated levels of cortisol and corticosterone in the LPS-induced model	1325:1399	The findings also showed that oral administration of S. boulardii significantly attenuated the elevated levels of cortisol and corticosterone in the LPS-induced model.
35358641	8	15	theme	open-field	999:1008	arg1	tests					1010:1014	open-field tests	999:1014	open-field tests	999:1014	Anxiety-like behaviors were assessed using the elevated plus-maze and open-field tests.
35358641	13	16	theme	HPA	1637:1639	arg1	axis					1641:1644	the HPA axis	1633:1644	the HPA axis	1633:1644	According to the present findings, S. boulardii can prevent LPS-induced anxiety-like behaviors, probably through modulation of the HPA axis and the gut microbiome.
35358641	9	17	theme	serotonin	1072:1080	arg1	levels					1036:1041	the serum levels	1026:1041	the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF)	1026:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	11	18	theme	cortisol	1348:1355	arg1	levels					1338:1343	the elevated levels	1325:1343	the elevated levels of cortisol and corticosterone in the LPS-induced model	1325:1399	The findings also showed that oral administration of S. boulardii significantly attenuated the elevated levels of cortisol and corticosterone in the LPS-induced model.
35358641	4	19	from	effects	524:530	arg1	behaviors					595:603	anxiety-like behaviors	582:603	anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats	582:647	The present study aimed to investigate the effects of Saccharomyces boulardii (Sb) administration on anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats.
35358641	3	20	theme	composition	468:478	arg1	modulation					435:444	modulation	435:444	modulation of the gut microbiome composition	435:478	Recent studies have reported that probiotics can attenuate anxiety-like behaviors by modulation of the gut microbiome composition.
35358641	4	21	from	lipopolysaccharide	616:633	arg1	rats					644:647	rats	644:647	rats	644:647	The present study aimed to investigate the effects of Saccharomyces boulardii (Sb) administration on anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats.
35358641	0	22	theme	lipopolysaccharide-induced	35:60	arg1	behaviors					75:83	lipopolysaccharide-induced anxiety-like behaviors	35:83	lipopolysaccharide-induced anxiety-like behaviors	35:83	Saccharomyces boulardii attenuates lipopolysaccharide-induced anxiety-like behaviors in rats.
35358641	13	23	theme	present	1523:1529	arg1	findings					1531:1538	the present findings	1519:1538	the present findings	1519:1538	According to the present findings, S. boulardii can prevent LPS-induced anxiety-like behaviors, probably through modulation of the HPA axis and the gut microbiome.
35358641	5	24	dep	groups	694:699	arg1	Sb + LPS					716:723	Sb + LPS	716:723	Sb + LPS	716:723	The animals were randomly divided into four groups (Control, LPS, Sb + LPS, and Sb).
35358641	5	24	dep	groups	694:699	arg1	Sb					730:731	Sb	730:731	Sb	730:731	The animals were randomly divided into four groups (Control, LPS, Sb + LPS, and Sb).
35358641	5	24	dep	groups	694:699	arg1	LPS					711:713	LPS	711:713	LPS	711:713	The animals were randomly divided into four groups (Control, LPS, Sb + LPS, and Sb).
35358641	5	24	dep	groups	694:699	arg1	Control					702:708	Control	702:708	Control	702:708	The animals were randomly divided into four groups (Control, LPS, Sb + LPS, and Sb).
35358641	5	24	dep	groups	694:699	arg1	groups					694:699	four groups	689:699	four groups (Control, LPS, Sb + LPS, and Sb)	689:732	The animals were randomly divided into four groups (Control, LPS, Sb + LPS, and Sb).
35358641	13	25	theme	anxiety-like	1578:1589	arg1	behaviors					1591:1599	LPS-induced anxiety-like behaviors	1566:1599	LPS-induced anxiety-like behaviors	1566:1599	According to the present findings, S. boulardii can prevent LPS-induced anxiety-like behaviors, probably through modulation of the HPA axis and the gut microbiome.
35358641	9	26	theme	brain-derived	1087:1099	arg1	BDNF					1122:1125	BDNF	1122:1125	BDNF	1122:1125	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	9	26	theme	brain-derived	1087:1099	arg1	factor					1114:1119	brain-derived neurotrophic factor	1087:1119	brain-derived neurotrophic factor (BDNF)	1087:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	2	27	theme	microbiota	200:209	arg1	dysbiosis					211:219	gut microbiota dysbiosis	196:219	gut microbiota dysbiosis	196:219	Exposure to stressors can cause gut microbiota dysbiosis and activate the hypothalamic-pituitary-adrenal (HPA) axis, leading to the secretion of glucocorticoids associated with anxiety.
35358641	9	28	theme	neurotrophic	1101:1112	arg1	BDNF					1122:1125	BDNF	1122:1125	BDNF	1122:1125	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	9	28	theme	neurotrophic	1101:1112	arg1	factor					1114:1119	brain-derived neurotrophic factor	1087:1119	brain-derived neurotrophic factor (BDNF)	1087:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	2	29	dep	hypothalamic-pituitary-adrenal	238:267	arg1	HPA					270:272	HPA	270:272	HPA	270:272	Exposure to stressors can cause gut microbiota dysbiosis and activate the hypothalamic-pituitary-adrenal (HPA) axis, leading to the secretion of glucocorticoids associated with anxiety.
35358641	3	30	theme	gut	453:455	arg1	composition					468:478	the gut microbiome composition	449:478	the gut microbiome composition	449:478	Recent studies have reported that probiotics can attenuate anxiety-like behaviors by modulation of the gut microbiome composition.
35358641	2	31	theme	gut	196:198	arg1	dysbiosis					211:219	gut microbiota dysbiosis	196:219	gut microbiota dysbiosis	196:219	Exposure to stressors can cause gut microbiota dysbiosis and activate the hypothalamic-pituitary-adrenal (HPA) axis, leading to the secretion of glucocorticoids associated with anxiety.
35358641	9	32	theme	factor	1114:1119	arg1	levels					1036:1041	the serum levels	1026:1041	the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF)	1026:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35358641	0	33	theme	anxiety-like	62:73	arg1	behaviors					75:83	lipopolysaccharide-induced anxiety-like behaviors	35:83	lipopolysaccharide-induced anxiety-like behaviors	35:83	Saccharomyces boulardii attenuates lipopolysaccharide-induced anxiety-like behaviors in rats.
35358641	3	34	theme	microbiome	457:466	arg1	composition					468:478	the gut microbiome composition	449:478	the gut microbiome composition	449:478	Recent studies have reported that probiotics can attenuate anxiety-like behaviors by modulation of the gut microbiome composition.
35358641	3	35	theme	anxiety-like	409:420	arg1	behaviors					422:430	anxiety-like behaviors	409:430	anxiety-like behaviors	409:430	Recent studies have reported that probiotics can attenuate anxiety-like behaviors by modulation of the gut microbiome composition.
35358641	11	36	theme	corticosterone	1361:1374	arg1	levels					1338:1343	the elevated levels	1325:1343	the elevated levels of cortisol and corticosterone in the LPS-induced model	1325:1399	The findings also showed that oral administration of S. boulardii significantly attenuated the elevated levels of cortisol and corticosterone in the LPS-induced model.
35358641	4	37	theme	administration	564:577	arg1	effects					524:530	the effects	520:530	the effects of Saccharomyces boulardii (Sb) administration on anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats	520:647	The present study aimed to investigate the effects of Saccharomyces boulardii (Sb) administration on anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats.
35358641	13	38	theme	axis	1641:1644	arg1	microbiome					1658:1667	the gut microbiome	1650:1667	the gut microbiome	1650:1667	According to the present findings, S. boulardii can prevent LPS-induced anxiety-like behaviors, probably through modulation of the HPA axis and the gut microbiome.
35358641	13	38	theme	axis	1641:1644	arg1	modulation					1619:1628	modulation	1619:1628	modulation of the HPA axis	1619:1644	According to the present findings, S. boulardii can prevent LPS-induced anxiety-like behaviors, probably through modulation of the HPA axis and the gut microbiome.
35358641	13	39	theme	LPS-induced	1566:1576	arg1	behaviors					1591:1599	LPS-induced anxiety-like behaviors	1566:1599	LPS-induced anxiety-like behaviors	1566:1599	According to the present findings, S. boulardii can prevent LPS-induced anxiety-like behaviors, probably through modulation of the HPA axis and the gut microbiome.
35358641	1	40	theme	dangerous	129:137	arg1	situations					152:161	dangerous or stressful situations	129:161	dangerous or stressful situations	129:161	Anxiety is the brain's response to dangerous or stressful situations.
35358641	12	41	theme	serum	1473:1477	arg1	serotonin					1479:1487	the serum serotonin and BDNF levels	1469:1503	serotonin	1479:1487	Moreover, S. boulardii alleviated the decremental effect of LPS on the serum serotonin and BDNF levels.
35358641	11	42	theme	oral	1264:1267	arg1	administration					1269:1282	oral administration	1264:1282	oral administration of S. boulardii	1264:1298	The findings also showed that oral administration of S. boulardii significantly attenuated the elevated levels of cortisol and corticosterone in the LPS-induced model.
35358641	12	43	theme	BDNF	1493:1496	arg1	levels					1498:1503	the serum serotonin and BDNF levels	1469:1503	levels	1498:1503	Moreover, S. boulardii alleviated the decremental effect of LPS on the serum serotonin and BDNF levels.
35358641	2	44	theme	hypothalamic-pituitary-adrenal	238:267	arg1	axis					275:278	the hypothalamic-pituitary-adrenal (HPA) axis	234:278	the hypothalamic-pituitary-adrenal (HPA) axis	234:278	Exposure to stressors can cause gut microbiota dysbiosis and activate the hypothalamic-pituitary-adrenal (HPA) axis, leading to the secretion of glucocorticoids associated with anxiety.
35358641	4	45	theme	anxiety-like	582:593	arg1	behaviors					595:603	anxiety-like behaviors	582:603	anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats	582:647	The present study aimed to investigate the effects of Saccharomyces boulardii (Sb) administration on anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats.
35358641	1	46	theme	stressful	142:150	arg1	situations					152:161	dangerous or stressful situations	129:161	dangerous or stressful situations	129:161	Anxiety is the brain's response to dangerous or stressful situations.
35358641	13	47	theme	gut	1654:1656	arg1	microbiome					1658:1667	the gut microbiome	1650:1667	the gut microbiome	1650:1667	According to the present findings, S. boulardii can prevent LPS-induced anxiety-like behaviors, probably through modulation of the HPA axis and the gut microbiome.
35358641	4	48	theme	present	485:491	arg1	study					493:497	The present study	481:497	The present study	481:497	The present study aimed to investigate the effects of Saccharomyces boulardii (Sb) administration on anxiety-like behaviors induced by lipopolysaccharide (LPS) in rats.
35358641	11	49	theme	LPS-induced	1383:1393	arg1	model					1395:1399	the LPS-induced model	1379:1399	the LPS-induced model	1379:1399	The findings also showed that oral administration of S. boulardii significantly attenuated the elevated levels of cortisol and corticosterone in the LPS-induced model.
35358641	8	50	theme	Anxiety-like	929:940	arg1	behaviors					942:950	Anxiety-like behaviors	929:950	Anxiety-like behaviors	929:950	Anxiety-like behaviors were assessed using the elevated plus-maze and open-field tests.
35358641	9	51	theme	serum	1030:1034	arg1	levels					1036:1041	the serum levels	1026:1041	the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF)	1026:1126	Besides, the serum levels of cortisol, corticosterone, serotonin, and brain-derived neurotrophic factor (BDNF) were measured.
35691703	3	0	theme	cohort	584:589	arg1	analysis					610:617	a secondary analysis	598:617	a secondary analysis of data collected in the prior STRONGER study	598:663	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	3	0	theme	cohort	584:589	arg1	study					591:595	a retrospective matched cohort study	560:595	a retrospective matched cohort study	560:595	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	0	1	theme	matched	204:210	arg1	STIL-STRONGER					175:187	STIL-STRONGER	175:187	STIL-STRONGER	175:187	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	0	1	theme	matched	204:210	arg1	study					219:223	a multicentre matched cohort study	190:223	a multicentre matched cohort study	190:223	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	10	2	theme	odds	1762:1765	arg1	0.39					1844:1847	0.39	1844:1847	0.39	1844:1847	After adjustment for unbalanced covariates, the adjusted odds ratio for the association between sugammadex use and the primary outcome was 0.39 (P<0.0001).
35691703	10	2	theme	odds	1762:1765	arg1	ratio					1767:1771	the adjusted odds ratio	1749:1771	the adjusted odds ratio for the association between sugammadex use and the primary outcome	1749:1838	After adjustment for unbalanced covariates, the adjusted odds ratio for the association between sugammadex use and the primary outcome was 0.39 (P<0.0001).
35691703	3	3	theme	secondary	600:608	arg1	analysis					610:617	a secondary analysis	598:617	a secondary analysis of data collected in the prior STRONGER study	598:663	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	3	3	theme	secondary	600:608	arg1	study					591:595	a retrospective matched cohort study	560:595	a retrospective matched cohort study	560:595	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	2	4	theme	increased	337:345	arg1	risk					347:350	increased risk	337:350	increased risk of postoperative pulmonary complications	337:391	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	5	5	theme	pulmonary	962:970	arg1	complications					972:984	postoperative pulmonary complications	948:984	postoperative pulmonary complications	948:984	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	11	6	theme	sugammadex	1977:1986	arg1	use					1970:1972	use	1970:1972	use of sugammadex	1970:1986	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	4	7	theme	Multicenter	694:704	arg1	Group					729:733	the Multicenter Perioperative Outcomes Group	690:733	the Multicenter Perioperative Outcomes Group	690:733	Data were obtained from the Multicenter Perioperative Outcomes Group.
35691703	0	8	theme	increased	126:134	arg1	risk					136:139	increased risk	126:139	increased risk	126:139	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	10	9	theme	adjusted	1753:1760	arg1	0.39					1844:1847	0.39	1844:1847	0.39	1844:1847	After adjustment for unbalanced covariates, the adjusted odds ratio for the association between sugammadex use and the primary outcome was 0.39 (P<0.0001).
35691703	10	9	theme	adjusted	1753:1760	arg1	ratio					1767:1771	the adjusted odds ratio	1749:1771	the adjusted odds ratio for the association between sugammadex use and the primary outcome	1749:1838	After adjustment for unbalanced covariates, the adjusted odds ratio for the association between sugammadex use and the primary outcome was 0.39 (P<0.0001).
35691703	8	10	theme	blocking	1503:1510	arg1	agent					1512:1516	neuromuscular blocking agent	1489:1516	neuromuscular blocking agent	1489:1516	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	4	11	theme	Outcomes	720:727	arg1	Group					729:733	the Multicenter Perioperative Outcomes Group	690:733	the Multicenter Perioperative Outcomes Group	690:733	Data were obtained from the Multicenter Perioperative Outcomes Group.
35691703	5	12	theme	non-emergency	791:803	arg1	surgery					805:811	non-emergency surgery	791:811	non-emergency surgery	791:811	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	5	13	theme	elevated	931:938	arg1	risk					940:943	elevated risk	931:943	elevated risk of postoperative pulmonary complications	931:984	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	6	14	theme	Anesthesiologists	1032:1048	arg1	Status					1059:1064	Anesthesiologists Physical Status 3 or 4	1032:1071	Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex	1032:1273	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	3	15	theme	STRONGER	650:657	arg1	study					659:663	the prior STRONGER study	640:663	the prior STRONGER study	640:663	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	9	16	theme	primary	1552:1558	arg1	outcome					1598:1604	The primary postoperative pulmonary complications outcome	1548:1604	The primary postoperative pulmonary complications outcome	1548:1604	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	8	17	theme	matched	1524:1530	arg1	pairs					1532:1536	3817 matched pairs	1519:1536	3817 matched pairs remained	1519:1545	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	8	17	theme	matched	1524:1530	arg1	RESULTS					1362:1368	RESULTS	1362:1368	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent	1362:1516	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	5	18	with	intubation	853:862	arg1	reversal					893:900	reversal	893:900	reversal	893:900	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	5	18	with	intubation	853:862	arg1	block					883:887	neuromuscular block	869:887	neuromuscular block	869:887	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	11	19	theme	development	2049:2059	arg1	risk					2030:2033	reduced risk	2022:2033	reduced risk of subsequent development of pneumonia or respiratory failure	2022:2095	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	9	20	theme	pulmonary	1574:1582	arg1	complications					1584:1596	postoperative pulmonary complications	1560:1596	The primary postoperative pulmonary complications outcome	1548:1604	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	6	21	theme	intra-abdominal	1120:1134	arg1	surgery					1136:1142	either intrathoracic or intra-abdominal surgery	1096:1142	either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex	1096:1273	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	5	22	theme	tracheal	844:851	arg1	intubation					853:862	tracheal intubation	844:862	tracheal intubation with neuromuscular block and reversal	844:900	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	6	23	theme	intrathoracic	1103:1115	arg1	surgery					1136:1142	either intrathoracic or intra-abdominal surgery	1096:1142	either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex	1096:1273	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	0	24	theme	postoperative	73:85	arg1	complications					97:109	postoperative pulmonary complications	73:109	postoperative pulmonary complications	73:109	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	8	25	theme	body	1425:1428	arg1	index					1435:1439	body mass index	1425:1439	body mass index	1425:1439	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	8	25	theme	body	1425:1428	arg1	age					1406:1408	age	1406:1408	age (within 5 yr)	1406:1422	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	2	26	from	risk	347:350	arg1	patients					325:332	patients	325:332	patients at increased risk of postoperative pulmonary complications	325:391	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	0	27	from	Association	0:10	arg1	patients					114:121	patients	114:121	patients at increased risk undergoing non-emergency surgery	114:172	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	0	27	from	Association	0:10	arg1	risk					136:139	increased risk	126:139	increased risk	126:139	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	9	28	theme	neostigmine	1622:1632	arg1	cases					1634:1638	224 neostigmine cases	1618:1638	224 neostigmine cases	1618:1638	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	11	29	from	risk	1910:1913	arg1	cohort					1878:1883	a cohort	1876:1883	a cohort of patients at increased risk for pulmonary complications	1876:1941	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	11	29	from	risk	1910:1913	arg1	patients					1888:1895	patients	1888:1895	patients at increased risk for pulmonary complications	1888:1941	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	6	30	dep	aged	1160:1163	arg1	yr					1169:1170	>80 yr	1165:1170	>80 yr	1165:1170	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	2	31	theme	neuromuscular	437:449	arg1	agent					475:479	neuromuscular blocking agent reversal agent	437:479	neuromuscular blocking agent reversal agent	437:479	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	7	32	theme	composite	1288:1296	arg1	outcome					1298:1304	The primary composite outcome	1276:1304	The primary composite outcome	1276:1304	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	7	32	theme	composite	1288:1296	arg1	occurrence					1314:1323	the occurrence	1310:1323	the occurrence of pneumonia or respiratory failure	1310:1359	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	0	33	theme	agent	39:43	arg1	choice					20:25	choice	20:25	choice of reversal agent for neuromuscular block	20:67	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	0	33	theme	agent	39:43	arg1	complications					97:109	postoperative pulmonary complications	73:109	postoperative pulmonary complications	73:109	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	2	34	theme	postoperative	500:512	arg1	complications					524:536	postoperative pulmonary complications	500:536	postoperative pulmonary complications	500:536	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	9	35	dep	occurred	1606:1613	arg1	%					1668:1668	5.9%	1665:1668	5.9%	1665:1668	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	9	35	dep	occurred	1606:1613	arg1	%					1676:1676	2.6%	1673:1676	2.6%	1673:1676	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	1	36	theme	major	307:311	arg1	surgery					313:319	major surgery	307:319	major surgery	307:319	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	9	37	theme	sugammadex	1647:1656	arg1	cases					1658:1662	100 sugammadex cases	1643:1662	100 sugammadex cases	1643:1662	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	8	38	theme	anatomic	1442:1449	arg1	region					1451:1456	anatomic region	1442:1456	anatomic region of surgery	1442:1467	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	8	38	theme	anatomic	1442:1449	arg1	age					1406:1408	age	1406:1408	age (within 5 yr)	1406:1422	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	5	39	theme	complications	972:984	arg1	risk					940:943	elevated risk	931:943	elevated risk of postoperative pulmonary complications	931:984	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	2	40	theme	agent	460:464	arg1	agent					475:479	neuromuscular blocking agent reversal agent	437:479	neuromuscular blocking agent reversal agent	437:479	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	0	41	theme	reversal	30:37	arg1	agent					39:43	reversal agent	30:43	reversal agent for neuromuscular block	30:67	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	2	42	theme	complications	524:536	arg1	development					485:495	development	485:495	development of postoperative pulmonary complications	485:536	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	2	42	theme	complications	524:536	arg1	agent					475:479	neuromuscular blocking agent reversal agent	437:479	neuromuscular blocking agent reversal agent	437:479	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	7	43	theme	pneumonia	1328:1336	arg1	outcome					1298:1304	The primary composite outcome	1276:1304	The primary composite outcome	1276:1304	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	7	43	theme	pneumonia	1328:1336	arg1	occurrence					1314:1323	the occurrence	1310:1323	the occurrence of pneumonia or respiratory failure	1310:1359	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	2	44	theme	complications	379:391	arg1	risk					347:350	increased risk	337:350	increased risk of postoperative pulmonary complications	337:391	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	11	45	theme	patients	1888:1895	arg1	cohort					1878:1883	a cohort	1876:1883	a cohort of patients at increased risk for pulmonary complications	1876:1941	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	2	46	theme	postoperative	355:367	arg1	complications					379:391	postoperative pulmonary complications	355:391	postoperative pulmonary complications	355:391	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	1	47	theme	pulmonary	251:259	arg1	complications					261:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	1	47	theme	pulmonary	251:259	arg1	source					281:286	a source	279:286	a source of morbidity after major surgery	279:319	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	11	48	theme	subsequent	2038:2047	arg1	development					2049:2059	subsequent development	2038:2059	subsequent development of pneumonia or respiratory failure	2038:2095	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	8	49	theme	neuromuscular	1489:1501	arg1	agent					1512:1516	neuromuscular blocking agent	1489:1516	neuromuscular blocking agent	1489:1516	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	11	50	theme	increased	1900:1908	arg1	risk					1910:1913	increased risk	1900:1913	increased risk for pulmonary complications	1900:1941	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	3	51	dep	METHODS	539:545	arg1	conducted					550:558	conducted	550:558	conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study	550:663	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	0	52	theme	multicentre	192:202	arg1	STIL-STRONGER					175:187	STIL-STRONGER	175:187	STIL-STRONGER	175:187	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	0	52	theme	multicentre	192:202	arg1	study					219:223	a multicentre matched cohort study	190:223	a multicentre matched cohort study	190:223	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	3	53	theme	matched	576:582	arg1	analysis					610:617	a secondary analysis	598:617	a secondary analysis of data collected in the prior STRONGER study	598:663	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	3	53	theme	matched	576:582	arg1	study					591:595	a retrospective matched cohort study	560:595	a retrospective matched cohort study	560:595	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	7	54	theme	respiratory	1341:1351	arg1	failure					1353:1359	respiratory failure	1341:1359	respiratory failure	1341:1359	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	0	55	theme	cohort	212:217	arg1	STIL-STRONGER					175:187	STIL-STRONGER	175:187	STIL-STRONGER	175:187	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	0	55	theme	cohort	212:217	arg1	study					219:223	a multicentre matched cohort study	190:223	a multicentre matched cohort study	190:223	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	10	56	theme	sugammadex	1801:1810	arg1	use					1812:1814	sugammadex use	1801:1814	sugammadex use	1801:1814	After adjustment for unbalanced covariates, the adjusted odds ratio for the association between sugammadex use and the primary outcome was 0.39 (P<0.0001).
35691703	1	57	theme	morbidity	291:299	arg1	complications					261:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	1	57	theme	morbidity	291:299	arg1	source					281:286	a source	279:286	a source of morbidity after major surgery	279:319	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	11	58	theme	respiratory	2077:2087	arg1	failure					2089:2095	respiratory failure	2077:2095	respiratory failure	2077:2095	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	11	59	from	CONCLUSIONS	1861:1871	arg1	cohort					1878:1883	a cohort	1876:1883	a cohort of patients at increased risk for pulmonary complications	1876:1941	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	10	60	theme	primary	1824:1830	arg1	outcome					1832:1838	the primary outcome	1820:1838	the primary outcome	1820:1838	After adjustment for unbalanced covariates, the adjusted odds ratio for the association between sugammadex use and the primary outcome was 0.39 (P<0.0001).
35691703	5	61	theme	postoperative	948:960	arg1	complications					972:984	postoperative pulmonary complications	948:984	postoperative pulmonary complications	948:984	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	9	62	dep	%	1668:1668	arg1	P<0.01					1696:1701	P<0.01	1696:1701	P<0.01	1696:1701	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	9	62	dep	%	1668:1668	arg1	odds					1679:1682	odds	1679:1682	odds	1679:1682	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	3	63	theme	data	622:625	arg1	analysis					610:617	a secondary analysis	598:617	a secondary analysis of data collected in the prior STRONGER study	598:663	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	3	63	theme	data	622:625	arg1	study					591:595	a retrospective matched cohort study	560:595	a retrospective matched cohort study	560:595	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	11	64	theme	failure	2089:2095	arg1	development					2049:2059	subsequent development	2038:2059	subsequent development of pneumonia or respiratory failure	2038:2095	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	2	65	theme	pulmonary	369:377	arg1	complications					379:391	postoperative pulmonary complications	355:391	postoperative pulmonary complications	355:391	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	0	66	theme	non-emergency	152:164	arg1	surgery					166:172	non-emergency surgery	152:172	non-emergency surgery	152:172	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	1	67	theme	BACKGROUND	226:235	arg1	complications					261:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	1	67	theme	BACKGROUND	226:235	arg1	source					281:286	a source	279:286	a source of morbidity after major surgery	279:319	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	6	68	with	reversal	1235:1242	arg1	sugammadex					1264:1273	sugammadex	1264:1273	sugammadex	1264:1273	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	6	68	with	reversal	1235:1242	arg1	neostigmine					1249:1259	neostigmine	1249:1259	neostigmine	1249:1259	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	5	69	with	anaesthesia	827:837	arg1	intubation					853:862	tracheal intubation	844:862	tracheal intubation with neuromuscular block and reversal	844:900	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	4	70	theme	Perioperative	706:718	arg1	Group					729:733	the Multicenter Perioperative Outcomes Group	690:733	the Multicenter Perioperative Outcomes Group	690:733	Data were obtained from the Multicenter Perioperative Outcomes Group.
35691703	11	71	theme	reduced	2022:2028	arg1	risk					2030:2033	reduced risk	2022:2033	reduced risk of subsequent development of pneumonia or respiratory failure	2022:2095	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	9	72	theme	postoperative	1560:1572	arg1	complications					1584:1596	postoperative pulmonary complications	1560:1596	The primary postoperative pulmonary complications outcome	1548:1604	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	11	73	theme	pneumonia	2064:2072	arg1	development					2049:2059	subsequent development	2038:2059	subsequent development of pneumonia or respiratory failure	2038:2095	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	9	74	dep	odds	1679:1682	arg1	ratio					1684:1688	ratio 0.41	1684:1693	ratio 0.41	1684:1693	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	9	75	theme	complications	1584:1596	arg1	outcome					1598:1604	The primary postoperative pulmonary complications outcome	1548:1604	The primary postoperative pulmonary complications outcome	1548:1604	The primary postoperative pulmonary complications outcome occurred in 224 neostigmine cases vs 100 sugammadex cases (5.9% vs 2.6%, odds ratio 0.41, P<0.01).
35691703	0	76	theme	pulmonary	87:95	arg1	complications					97:109	postoperative pulmonary complications	73:109	postoperative pulmonary complications	73:109	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	5	77	theme	general	819:825	arg1	anaesthesia					827:837	general anaesthesia	819:837	general anaesthesia	819:837	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	5	78	dep	aged	759:762	arg1	yr					767:768	18 yr	764:768	18 yr	764:768	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	7	79	theme	primary	1280:1286	arg1	outcome					1298:1304	The primary composite outcome	1276:1304	The primary composite outcome	1276:1304	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	7	79	theme	primary	1280:1286	arg1	occurrence					1314:1323	the occurrence	1310:1323	the occurrence of pneumonia or respiratory failure	1310:1359	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	11	80	from	cohort	1878:1883	arg1	risk					1910:1913	increased risk	1900:1913	increased risk for pulmonary complications	1900:1941	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	8	81	theme	mass	1430:1433	arg1	index					1435:1439	body mass index	1425:1439	body mass index	1425:1439	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	8	81	theme	mass	1430:1433	arg1	age					1406:1408	age	1406:1408	age (within 5 yr)	1406:1422	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	11	82	theme	pulmonary	1919:1927	arg1	complications					1929:1941	pulmonary complications	1919:1941	pulmonary complications	1919:1941	CONCLUSIONS In a cohort of patients at increased risk for pulmonary complications compared with neostigmine, use of sugammadex was independently associated with reduced risk of subsequent development of pneumonia or respiratory failure.
35691703	6	83	from	Status	1059:1064	arg1	patients					1076:1083	patients	1076:1083	patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex	1076:1273	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	2	84	theme	reversal	466:473	arg1	agent					475:479	neuromuscular blocking agent reversal agent	437:479	neuromuscular blocking agent reversal agent	437:479	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	5	85	theme	neuromuscular	869:881	arg1	block					883:887	neuromuscular block	869:887	neuromuscular block	869:887	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	2	86	theme	blocking	451:458	arg1	agent					475:479	neuromuscular blocking agent reversal agent	437:479	neuromuscular blocking agent reversal agent	437:479	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	0	87	theme	neuromuscular	49:61	arg1	block					63:67	neuromuscular block	49:67	neuromuscular block	49:67	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	6	88	theme	Physical	1050:1057	arg1	Status					1059:1064	Anesthesiologists Physical Status 3 or 4	1032:1071	Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex	1032:1273	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	2	89	theme	pulmonary	514:522	arg1	complications					524:536	postoperative pulmonary complications	500:536	postoperative pulmonary complications	500:536	In patients at increased risk of postoperative pulmonary complications we sought to assess the association between neuromuscular blocking agent reversal agent and development of postoperative pulmonary complications.
35691703	5	90	theme	Included	736:743	arg1	patients					745:752	Included patients	736:752	Included patients	736:752	Included patients were aged 18 yr and older undergoing non-emergency surgery under general anaesthesia with tracheal intubation with neuromuscular block and reversal, who were predicted to be at elevated risk of postoperative pulmonary complications.
35691703	8	91	theme	surgery	1461:1467	arg1	region					1451:1456	anatomic region	1442:1456	anatomic region of surgery	1442:1467	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	8	91	theme	surgery	1461:1467	arg1	age					1406:1408	age	1406:1408	age (within 5 yr)	1406:1422	RESULTS After matching by institution, sex, age (within 5 yr), body mass index, anatomic region of surgery, comorbidities, and neuromuscular blocking agent, 3817 matched pairs remained.
35691703	3	92	theme	retrospective	562:574	arg1	analysis					610:617	a secondary analysis	598:617	a secondary analysis of data collected in the prior STRONGER study	598:663	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	3	92	theme	retrospective	562:574	arg1	study					591:595	a retrospective matched cohort study	560:595	a retrospective matched cohort study	560:595	METHODS We conducted a retrospective matched cohort study, a secondary analysis of data collected in the prior STRONGER study.
35691703	0	93	dep	Association	0:10	arg1	STIL-STRONGER					175:187	STIL-STRONGER	175:187	STIL-STRONGER	175:187	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	0	93	dep	Association	0:10	arg1	study					219:223	a multicentre matched cohort study	190:223	a multicentre matched cohort study	190:223	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	6	94	dep	h.	1208:1209	arg1	defined					1224:1230	defined	1224:1230	were defined by reversal with neostigmine or sugammadex	1219:1273	This risk was defined as American Society of Anesthesiologists Physical Status 3 or 4 in patients undergoing either intrathoracic or intra-abdominal surgery who were either aged >80 yr or underwent a procedure lasting >2 h. Cohorts were defined by reversal with neostigmine or sugammadex.
35691703	10	95	theme	unbalanced	1726:1735	arg1	covariates					1737:1746	unbalanced covariates	1726:1746	unbalanced covariates	1726:1746	After adjustment for unbalanced covariates, the adjusted odds ratio for the association between sugammadex use and the primary outcome was 0.39 (P<0.0001).
35691703	1	96	theme	Postoperative	237:249	arg1	complications					261:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications	226:273	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	1	96	theme	Postoperative	237:249	arg1	source					281:286	a source	279:286	a source of morbidity after major surgery	279:319	BACKGROUND Postoperative pulmonary complications are a source of morbidity after major surgery.
35691703	7	97	theme	failure	1353:1359	arg1	outcome					1298:1304	The primary composite outcome	1276:1304	The primary composite outcome	1276:1304	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	7	97	theme	failure	1353:1359	arg1	occurrence					1314:1323	the occurrence	1310:1323	the occurrence of pneumonia or respiratory failure	1310:1359	The primary composite outcome was the occurrence of pneumonia or respiratory failure.
35691703	0	98	from	risk	136:139	arg1	Association					0:10	Association	0:10	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.	0:224	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35691703	0	98	from	risk	136:139	arg1	patients					114:121	patients	114:121	patients at increased risk undergoing non-emergency surgery	114:172	Association between choice of reversal agent for neuromuscular block and postoperative pulmonary complications in patients at increased risk undergoing non-emergency surgery: STIL-STRONGER, a multicentre matched cohort study.
35014252	10	0	theme	fibroblast	1904:1913	arg1	cells					1915:1919	human fibroblast cells	1898:1919	human fibroblast cells	1898:1919	Their therapeutic efficacy and biocompatibility are also evident from the studies on human fibroblast cells.
35014252	3	1	theme	wound	541:545	arg1	healing					547:553	wound healing	541:553	wound healing	541:553	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	6	2	theme	maximum	1155:1161	arg1	efficiency					1177:1186	a maximum encapsulation efficiency	1153:1186	a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases	1153:1307	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	4	3	theme	single-step	628:638	arg1	method					656:661	a facile single-step and water-based method	619:661	a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release	619:743	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	6	4	theme	anti-inflammatory	1231:1247	arg1	diclofenac					1198:1207	20-85% diclofenac	1191:1207	20-85% diclofenac (DCF)	1191:1213	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	6	4	theme	anti-inflammatory	1231:1247	arg1	drug					1249:1252	a nonsteroidal anti-inflammatory drug	1216:1252	a nonsteroidal anti-inflammatory drug	1216:1252	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	0	5	theme	One-Step	0:7	arg1	Fabrication					9:19	One-Step Fabrication	0:19	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.	0:107	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	5	6	theme	acetate	860:866	arg1	beads					868:872	hydrophobic solid and spherical cellulose acetate beads	818:872	hydrophobic solid and spherical cellulose acetate beads	818:872	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	7	7	theme	release	1399:1405	arg1	medium					1407:1412	the release medium	1395:1412	the release medium	1395:1412	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	4	8	theme	water-based	644:654	arg1	method					656:661	a facile single-step and water-based method	619:661	a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release	619:743	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	11	9	theme	sustainable	2006:2016	arg1	systems					2062:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	We anticipate that this platform could support and inspire the development of novel sustainable and effective polysaccharide-based delivery systems.
35014252	11	10	theme	polysaccharide-based	2032:2051	arg1	systems					2062:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	We anticipate that this platform could support and inspire the development of novel sustainable and effective polysaccharide-based delivery systems.
35014252	6	11	theme	cellulose	1124:1132	arg1	structures					1134:1143	the hollow cellulose structures	1113:1143	the hollow cellulose structures	1113:1143	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	5	12	theme	solid	830:834	arg1	beads					868:872	hydrophobic solid and spherical cellulose acetate beads	818:872	hydrophobic solid and spherical cellulose acetate beads	818:872	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	5	13	theme	time	1056:1059	arg1	increment					1017:1025	a stepwise increment	1006:1025	a stepwise increment of temperature and treatment time	1006:1059	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	5	14	theme	spherical	840:848	arg1	beads					868:872	hydrophobic solid and spherical cellulose acetate beads	818:872	hydrophobic solid and spherical cellulose acetate beads	818:872	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	0	15	from	Application	58:68	arg1	Delivery					99:106	pH-Responsive Therapeutic Delivery	73:106	pH-Responsive Therapeutic Delivery	73:106	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	4	16	theme	hollow	686:691	arg1	beads					713:717	hollow spherical cellulose beads	686:717	hollow spherical cellulose beads	686:717	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	5	17	theme	stepwise	1008:1015	arg1	increment					1017:1025	a stepwise increment	1006:1025	a stepwise increment of temperature and treatment time	1006:1059	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	1	18	theme	greater	121:127	arg1	sustainability					129:142	greater sustainability	121:142	greater sustainability	121:142	The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches.
35014252	11	19	theme	systems	2062:2068	arg1	development					1985:1995	the development	1981:1995	the development of novel sustainable and effective polysaccharide-based delivery systems	1981:2068	We anticipate that this platform could support and inspire the development of novel sustainable and effective polysaccharide-based delivery systems.
35014252	6	20	theme	diclofenac	1198:1207	arg1	efficiency					1177:1186	a maximum encapsulation efficiency	1153:1186	a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases	1153:1307	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	7	21	theme	DCF	1332:1334	arg1	DCF					1332:1334	DCF	1332:1334	DCF	1332:1334	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	7	21	theme	DCF	1332:1334	arg1	amount					1322:1327	The maximum amount	1310:1327	The maximum amount of DCF released in vitro	1310:1352	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	4	22	theme	cellulose	703:711	arg1	beads					713:717	hollow spherical cellulose beads	686:717	hollow spherical cellulose beads	686:717	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	5	23	theme	hollow	935:940	arg1	interior					942:949	a hollow interior	933:949	a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm)	933:1001	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	6	24	theme	20-85	1191:1195	arg1	%					1196:1196	%	1196:1196	%	1196:1196	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	2	25	theme	biobased	257:264	arg1	materials					266:274	biobased materials	257:274	biobased materials	257:274	The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand.
35014252	9	26	theme	excellent	1694:1702	arg1	characteristics					1704:1718	excellent characteristics	1694:1718	excellent characteristics for transport across the gastrointestinal tract	1694:1766	Overall, we show that the modified beads exhibit excellent characteristics for transport across the gastrointestinal tract and enhance the bioavailability of the drug.
35014252	5	27	dep	interior	942:949	arg1	thickness					957:965	wall thickness	952:965	wall thickness: 150 μm and inner diameter: 650 μm	952:1000	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	0	28	theme	pH-Responsive	73:85	arg1	Delivery					99:106	pH-Responsive Therapeutic Delivery	73:106	pH-Responsive Therapeutic Delivery	73:106	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	1	29	theme	polymeric	167:175	arg1	systems					191:197	polymeric drug delivery systems	167:197	polymeric drug delivery systems	167:197	The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches.
35014252	3	30	theme	crucial	364:370	arg1	part					372:375	A crucial part	362:375	A crucial part of this	362:383	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	2	31	theme	targeted	301:308	arg1	delivery					322:329	targeted therapeutic delivery	301:329	targeted therapeutic delivery	301:329	The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand.
35014252	5	32	theme	150	968:970	arg1	μm					972:973	μm	972:973	μm	972:973	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	3	33	theme	structures	433:442	arg1	development					400:410	the development	396:410	the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth	396:588	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	7	34	theme	maximum	1314:1320	arg1	DCF					1332:1334	DCF	1332:1334	DCF	1332:1334	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	7	34	theme	maximum	1314:1320	arg1	amount					1322:1327	The maximum amount	1310:1327	The maximum amount of DCF released in vitro	1310:1352	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	9	35	theme	gastrointestinal	1745:1760	arg1	tract					1762:1766	the gastrointestinal tract	1741:1766	the gastrointestinal tract	1741:1766	Overall, we show that the modified beads exhibit excellent characteristics for transport across the gastrointestinal tract and enhance the bioavailability of the drug.
35014252	8	36	theme	release	1459:1465	arg1	patterns					1467:1474	DCF release patterns	1455:1474	DCF release patterns	1455:1474	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	5	37	with	structures	917:926	arg1	interior					942:949	a hollow interior	933:949	a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm)	933:1001	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	5	38	theme	base-catalyzed	789:802	arg1	deprotection					804:815	base-catalyzed deprotection	789:815	base-catalyzed deprotection	789:815	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	4	39	theme	drug	732:735	arg1	release					737:743	targeted drug release	723:743	targeted drug release	723:743	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	1	40	theme	drug	177:180	arg1	systems					191:197	polymeric drug delivery systems	167:197	polymeric drug delivery systems	167:197	The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches.
35014252	0	41	theme	Spherical	31:39	arg1	Beads					51:55	Hollow Spherical Cellulose Beads	24:55	Hollow Spherical Cellulose Beads	24:55	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	8	42	theme	specific	1498:1505	arg1	values					1510:1515	specific pH values	1498:1515	specific pH values	1498:1515	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	3	43	theme	active	491:496	arg1	substances					498:507	active substances	491:507	active substances	491:507	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	0	44	theme	Beads	51:55	arg1	Fabrication					9:19	One-Step Fabrication	0:19	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.	0:107	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	8	45	from	values	1510:1515	arg1	models					1488:1493	kinetic models	1480:1493	kinetic models	1480:1493	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	8	45	from	values	1510:1515	arg1	patterns					1467:1474	DCF release patterns	1455:1474	DCF release patterns	1455:1474	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	9	46	theme	drug	1807:1810	arg1	bioavailability					1784:1798	the bioavailability	1780:1798	the bioavailability of the drug	1780:1810	Overall, we show that the modified beads exhibit excellent characteristics for transport across the gastrointestinal tract and enhance the bioavailability of the drug.
35014252	4	47	theme	pH	769:770	arg1	stimuli					772:778	external pH stimuli	760:778	external pH stimuli	760:778	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	8	48	dep	patterns	1467:1474	arg1	the					1451:1453	the	1451:1453	the	1451:1453	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	3	49	dep	biocompatible	453:465	arg1	pH-responsive					468:480	pH-responsive	468:480	pH-responsive	468:480	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	6	50	theme	uptake	1094:1099	arg1	properties					1101:1110	the pH-responsive fluid uptake properties	1070:1110	the pH-responsive fluid uptake properties	1070:1110	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	1	51	theme	delivery	182:189	arg1	systems					191:197	polymeric drug delivery systems	167:197	polymeric drug delivery systems	167:197	The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches.
35014252	6	52	theme	pH-responsive	1074:1086	arg1	properties					1101:1110	the pH-responsive fluid uptake properties	1070:1110	the pH-responsive fluid uptake properties	1070:1110	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	8	53	theme	external	1610:1617	arg1	stimuli					1636:1642	external environmental pH stimuli	1610:1642	external environmental pH stimuli	1610:1642	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	8	54	theme	diffusion-	1539:1548	arg1	profile					1574:1580	a diffusion- and swelling-controlled profile	1537:1580	a diffusion- and swelling-controlled profile	1537:1580	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	2	55	theme	therapeutic	310:320	arg1	delivery					322:329	targeted therapeutic delivery	301:329	targeted therapeutic delivery	301:329	The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand.
35014252	8	56	theme	pH	1633:1634	arg1	stimuli					1636:1642	external environmental pH stimuli	1610:1642	external environmental pH stimuli	1610:1642	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	11	57	theme	novel	2000:2004	arg1	systems					2062:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	We anticipate that this platform could support and inspire the development of novel sustainable and effective polysaccharide-based delivery systems.
35014252	7	58	theme	medium	1407:1412	arg1	pH					1389:1390	the pH	1385:1390	the pH of the release medium	1385:1412	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	7	59	dep	100	1375:1377	arg1	to					1372:1373	to	1372:1373	to	1372:1373	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	8	60	theme	swelling-controlled	1554:1572	arg1	profile					1574:1580	a diffusion- and swelling-controlled profile	1537:1580	a diffusion- and swelling-controlled profile	1537:1580	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	10	61	theme	human	1898:1902	arg1	cells					1915:1919	human fibroblast cells	1898:1919	human fibroblast cells	1898:1919	Their therapeutic efficacy and biocompatibility are also evident from the studies on human fibroblast cells.
35014252	3	62	theme	pain	528:531	arg1	relief					533:538	pain relief	528:538	pain relief	528:538	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	5	63	theme	cellulose	850:858	arg1	beads					868:872	hydrophobic solid and spherical cellulose acetate beads	818:872	hydrophobic solid and spherical cellulose acetate beads	818:872	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	4	64	theme	facile	621:626	arg1	method					656:661	a facile single-step and water-based method	619:661	a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release	619:743	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	6	65	theme	nonsteroidal	1218:1229	arg1	diclofenac					1198:1207	20-85% diclofenac	1191:1207	20-85% diclofenac (DCF)	1191:1213	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	6	65	theme	nonsteroidal	1218:1229	arg1	drug					1249:1252	a nonsteroidal anti-inflammatory drug	1216:1252	a nonsteroidal anti-inflammatory drug	1216:1252	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	3	66	theme	tissue	556:561	arg1	regeneration					563:574	tissue regeneration	556:574	tissue regeneration	556:574	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	1	67	theme	systems	191:197	arg1	development					152:162	the development	148:162	the development of polymeric drug delivery systems	148:197	The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches.
35014252	1	67	theme	systems	191:197	arg1	path					113:116	The path	109:116	The path to greater sustainability	109:142	The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches.
35014252	1	68	theme	innovative	208:217	arg1	approaches					219:228	innovative approaches	208:228	innovative approaches	208:228	The path to greater sustainability and the development of polymeric drug delivery systems requires innovative approaches.
35014252	11	69	theme	delivery	2053:2060	arg1	systems					2062:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	We anticipate that this platform could support and inspire the development of novel sustainable and effective polysaccharide-based delivery systems.
35014252	5	70	theme	cellulose	907:915	arg1	structures					917:926	hydrophilic cellulose structures	895:926	hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm)	895:1001	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	2	71	dep	adaptation	235:244	arg1	The					231:233	The	231:233	The	231:233	The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand.
35014252	5	72	theme	treatment	1046:1054	arg1	time					1056:1059	treatment time	1046:1059	treatment time	1046:1059	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	10	73	from	studies	1887:1893	arg1	cells					1915:1919	human fibroblast cells	1898:1919	human fibroblast cells	1898:1919	Their therapeutic efficacy and biocompatibility are also evident from the studies on human fibroblast cells.
35014252	10	73	from	studies	1887:1893	arg1	evident					1870:1876	evident	1870:1876	evident	1870:1876	Their therapeutic efficacy and biocompatibility are also evident from the studies on human fibroblast cells.
35014252	11	74	theme	effective	2022:2030	arg1	systems					2062:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	novel sustainable and effective polysaccharide-based delivery systems	2000:2068	We anticipate that this platform could support and inspire the development of novel sustainable and effective polysaccharide-based delivery systems.
35014252	6	75	theme	inflammatory	1287:1298	arg1	diseases					1300:1307	inflammatory diseases	1287:1307	inflammatory diseases	1287:1307	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	6	76	theme	encapsulation	1163:1175	arg1	efficiency					1177:1186	a maximum encapsulation efficiency	1153:1186	a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases	1153:1307	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	9	77	theme	modified	1671:1678	arg1	beads					1680:1684	the modified beads	1667:1684	the modified beads	1667:1684	Overall, we show that the modified beads exhibit excellent characteristics for transport across the gastrointestinal tract and enhance the bioavailability of the drug.
35014252	6	78	theme	%	1196:1196	arg1	DCF					1210:1212	DCF	1210:1212	DCF	1210:1212	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	6	78	theme	%	1196:1196	arg1	diclofenac					1198:1207	20-85% diclofenac	1191:1207	20-85% diclofenac (DCF)	1191:1213	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	6	78	theme	%	1196:1196	arg1	drug					1249:1252	a nonsteroidal anti-inflammatory drug	1216:1252	a nonsteroidal anti-inflammatory drug	1216:1252	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	4	79	theme	spherical	693:701	arg1	beads					713:717	hollow spherical cellulose beads	686:717	hollow spherical cellulose beads	686:717	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	5	80	theme	temperature	1030:1040	arg1	increment					1017:1025	a stepwise increment	1006:1025	a stepwise increment of temperature and treatment time	1006:1059	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	2	81	theme	materials	266:274	arg1	use					250:252	use	250:252	use	250:252	The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand.
35014252	2	81	theme	materials	266:274	arg1	adaptation					235:244	adaptation	235:244	adaptation	235:244	The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand.
35014252	5	82	theme	hydrophobic	818:828	arg1	beads					868:872	hydrophobic solid and spherical cellulose acetate beads	818:872	hydrophobic solid and spherical cellulose acetate beads	818:872	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	5	83	dep	thickness	957:965	arg1	diameter					985:992	150 μm and inner diameter	968:992	wall thickness: 150 μm and inner diameter: 650 μm	952:1000	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	5	83	dep	thickness	957:965	arg1	μm					999:1000	650 μm	995:1000	wall thickness: 150 μm and inner diameter: 650 μm	952:1000	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	0	84	theme	Therapeutic	87:97	arg1	Delivery					99:106	pH-Responsive Therapeutic Delivery	73:106	pH-Responsive Therapeutic Delivery	73:106	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	5	85	theme	hydrophilic	895:905	arg1	structures					917:926	hydrophilic cellulose structures	895:926	hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm)	895:1001	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	6	86	theme	hollow	1117:1122	arg1	structures					1134:1143	the hollow cellulose structures	1113:1143	the hollow cellulose structures	1113:1143	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	8	87	theme	DCF	1455:1457	arg1	patterns					1467:1474	DCF release patterns	1455:1474	DCF release patterns	1455:1474	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	5	88	theme	μm	972:973	arg1	diameter					985:992	150 μm and inner diameter	968:992	wall thickness: 150 μm and inner diameter: 650 μm	952:1000	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	8	89	theme	kinetic	1480:1486	arg1	models					1488:1493	kinetic models	1480:1493	kinetic models	1480:1493	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	4	90	theme	beads	713:717	arg1	fabrication					671:681	the fabrication	667:681	the fabrication of hollow spherical cellulose beads	667:717	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	3	91	theme	hollow	426:431	arg1	structures					433:442	porous and hollow structures	415:442	porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth	415:588	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	0	92	theme	Hollow	24:29	arg1	Beads					51:55	Hollow Spherical Cellulose Beads	24:55	Hollow Spherical Cellulose Beads	24:55	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	7	93	dep	pH	1429:1430	arg1	to					1436:1437	to	1436:1437	to	1436:1437	The maximum amount of DCF released in vitro increased from 20 to 100% when the pH of the release medium increased from pH 1.2 to 7.4.
35014252	5	94	theme	wall	952:955	arg1	thickness					957:965	wall thickness	952:965	wall thickness: 150 μm and inner diameter: 650 μm	952:1000	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	3	95	theme	this	380:383	arg1	part					372:375	A crucial part	362:375	A crucial part of this	362:383	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	10	96	theme	therapeutic	1819:1829	arg1	efficacy					1831:1838	Their therapeutic efficacy	1813:1838	Their therapeutic efficacy	1813:1838	Their therapeutic efficacy and biocompatibility are also evident from the studies on human fibroblast cells.
35014252	4	97	theme	targeted	723:730	arg1	release					737:743	targeted drug release	723:743	targeted drug release	723:743	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	0	98	theme	Cellulose	41:49	arg1	Beads					51:55	Hollow Spherical Cellulose Beads	24:55	Hollow Spherical Cellulose Beads	24:55	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	3	99	theme	porous	415:420	arg1	structures					433:442	porous and hollow structures	415:442	porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth	415:588	A crucial part of this relates to the development of porous and hollow structures that are biocompatible, pH-responsive, deliver active substances, and contribute to pain relief, wound healing, tissue regeneration, and so forth.
35014252	8	100	theme	pH	1507:1508	arg1	values					1510:1515	specific pH values	1498:1515	specific pH values	1498:1515	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
35014252	2	101	theme	high	349:352	arg1	demand					354:359	high demand	349:359	high demand	349:359	The adaptation and use of biobased materials for applications such as targeted therapeutic delivery is, therefore, in high demand.
35014252	5	102	theme	inner	979:983	arg1	diameter					985:992	150 μm and inner diameter	968:992	wall thickness: 150 μm and inner diameter: 650 μm	952:1000	Through base-catalyzed deprotection, hydrophobic solid and spherical cellulose acetate beads are transformed into hydrophilic cellulose structures with a hollow interior (wall thickness: 150 μm and inner diameter: 650 μm) by a stepwise increment of temperature and treatment time.
35014252	4	103	theme	external	760:767	arg1	stimuli					772:778	external pH stimuli	760:778	external pH stimuli	760:778	In this study, we developed a facile single-step and water-based method for the fabrication of hollow spherical cellulose beads for targeted drug release in response to external pH stimuli.
35014252	0	104	dep	Fabrication	9:19	arg1	Application					58:68	Application	58:68	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.	0:107	One-Step Fabrication of Hollow Spherical Cellulose Beads: Application in pH-Responsive Therapeutic Delivery.
35014252	6	105	theme	fluid	1088:1092	arg1	properties					1101:1110	the pH-responsive fluid uptake properties	1070:1110	the pH-responsive fluid uptake properties	1070:1110	Besides the pH-responsive fluid uptake properties, the hollow cellulose structures exhibit a maximum encapsulation efficiency of 20-85% diclofenac (DCF), a nonsteroidal anti-inflammatory drug, used commonly to treat pain and inflammatory diseases.
35014252	8	106	theme	environmental	1619:1631	arg1	stimuli					1636:1642	external environmental pH stimuli	1610:1642	external environmental pH stimuli	1610:1642	As for the DCF release patterns and kinetic models at specific pH values, the release showed a diffusion- and swelling-controlled profile, effortlessly fine-tuned by external environmental pH stimuli.
36971207	7	0	theme	fed	1219:1221	arg1	sows					1223:1226	the PP fed sows	1212:1226	the PP fed sows	1212:1226	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	11	1	theme	BSG	1951:1953	arg1	MJ/d					1980:1983	-0.22 to -0.69 MJ/d	1965:1983	-0.22 to -0.69 MJ/d	1965:1983	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	11	1	theme	BSG	1951:1953	arg1	sows					1959:1962	BSG fed sows	1951:1962	BSG fed sows (-0.22 to -0.69 MJ/d)	1951:1984	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	14	2	theme	sow	2438:2440	arg1	diets					2442:2446	sow diets	2438:2446	sow diets	2438:2446	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	9	3	theme	energy	1519:1524	arg1	content					1526:1532	digestible and metabolizable energy content	1490:1532	digestible and metabolizable energy content of the FRCP ingredients	1490:1556	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	11	4	theme	SR	2012:2013	arg1	sows					2019:2022	SR fed sows	2012:2022	SR fed sows	2012:2022	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	12	5	dep	potential	2126:2134	arg1	replace					2146:2152	replace	2146:2152	to partly replace high value grain crops due to high ATTD of all nutrients	2136:2209	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	12	5	dep	potential	2126:2134	arg1	utilize					2244:2250	utilize	2244:2250	because sows can efficiently utilize energy and protein	2215:2269	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	8	6	theme	nutrients	1358:1366	arg1	intermediate					1384:1395	intermediate	1384:1395	intermediate	1384:1395	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	8	6	theme	nutrients	1358:1366	arg1	ATTDs					1345:1349	the ATTDs	1341:1349	the ATTDs of all nutrients and energy	1341:1377	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	14	7	contain	have	2401:2404	arg1	BSG					2392:2394	BSG	2392:2394	BSG	2392:2394	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	14	7	contain	have	2401:2404	arg1	pulp					2383:2386	Potato pulp	2376:2386	Potato pulp	2376:2386	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	14	7	contain	have	2401:2404	arg2	potential					2410:2418	the potential to be included in sow diets	2406:2446	the potential to be included in sow diets	2406:2446	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	10	8	theme	P	1818:1818	arg1	sows					1812:1815	fed sows	1808:1815	fed sows (P < 0.05)	1808:1826	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	10	8	theme	P	1818:1818	arg1	<					1820:1820	P < 0.05	1818:1825	P < 0.05	1818:1825	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	4	9	theme	different	641:649	arg1	FRCP					675:678	FRCP	675:678	FRCP	675:678	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	9	theme	different	641:649	arg1	co-products					662:672	six different fiber-rich co-products	637:672	six different fiber-rich co-products (FRCP)	637:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	12	10	theme	high	2154:2157	arg1	crops					2171:2175	high value grain crops	2154:2175	high value grain crops due to high ATTD of all nutrients	2154:2209	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	8	11	theme	energy	1372:1377	arg1	intermediate					1384:1395	intermediate	1384:1395	intermediate	1384:1395	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	8	11	theme	energy	1372:1377	arg1	ATTDs					1345:1349	the ATTDs	1341:1349	the ATTDs of all nutrients and energy	1341:1377	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	5	12	theme	sugar	763:767	arg1	SBP					780:782	SBP	780:782	SBP	780:782	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	12	theme	sugar	763:767	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	12	theme	sugar	763:767	arg1	pulp					774:777	sugar beet pulp	763:777	sugar beet pulp (SBP)	763:783	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	9	13	from	variation	1477:1485	arg1	content					1526:1532	digestible and metabolizable energy content	1490:1532	digestible and metabolizable energy content of the FRCP ingredients	1490:1556	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	9	14	theme	FRCP	1541:1544	arg1	ingredients					1546:1556	the FRCP ingredients	1537:1556	the FRCP ingredients	1537:1556	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	6	15	theme	days	1045:1048	arg1	period					1030:1035	a total collection period	1011:1035	a total collection period	1011:1035	The collection period consisted of a total collection period of five days, of which two days were in a respiration chamber.
36971207	2	16	theme	diverse	295:301	arg1	composition					303:313	diverse composition	295:313	diverse composition	295:313	The co-products are typically high in fiber and with diverse composition.
36971207	1	17	theme	concentrated	187:198	arg1	crops					217:221	concentrated high value grain crops	187:221	concentrated high value grain crops in diets for sows	187:239	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	4	18	theme	non-lactating	614:626	arg1	sows					628:631	empty non-lactating sows	608:631	empty non-lactating sows fed six different fiber-rich co-products (FRCP)	608:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	5	19	theme	spent	690:694	arg1	BSG					703:705	BSG	703:705	BSG	703:705	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	19	theme	spent	690:694	arg1	grain					696:700	spent grain	690:700	spent grain (BSG)	690:706	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	19	theme	spent	690:694	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	1	20	theme	value	205:209	arg1	crops					217:221	concentrated high value grain crops	187:221	concentrated high value grain crops in diets for sows	187:239	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	12	21	theme	sow	2081:2083	arg1	perspective					2093:2103	a sow feeding perspective	2079:2103	a sow feeding perspective	2079:2103	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	0	22	from	utilization	77:87	arg1	sows					92:95	sows	92:95	sows	92:95	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	0	23	from	digestibility	59:71	arg1	sows					92:95	sows	92:95	sows	92:95	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	6	24	theme	period	1030:1035	arg1	chamber					1091:1097	a respiration chamber	1077:1097	a respiration chamber	1077:1097	The collection period consisted of a total collection period of five days, of which two days were in a respiration chamber.
36971207	1	25	theme	food	119:122	arg1	industries					141:150	the food and agricultural industries	115:150	the food and agricultural industries	115:150	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	2	26	from	high	272:275	arg1	fiber					280:284	fiber	280:284	fiber	280:284	The co-products are typically high in fiber and with diverse composition.
36971207	6	27	theme	total	1013:1017	arg1	period					1030:1035	a total collection period	1011:1035	a total collection period	1011:1035	The collection period consisted of a total collection period of five days, of which two days were in a respiration chamber.
36971207	11	28	theme	P	2062:2062	arg1	<					2064:2064	P < 0.001	2062:2070	P < 0.001	2062:2070	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	8	29	theme	fed	1425:1427	arg1	sows					1429:1432	SR fed sows	1422:1432	SR fed sows (P < 0.01)	1422:1443	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	8	29	theme	fed	1425:1427	arg1	<					1437:1437	P < 0.01	1435:1442	P < 0.01	1435:1442	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	1	30	theme	agricultural	128:139	arg1	industries					141:150	the food and agricultural industries	115:150	the food and agricultural industries	115:150	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	2	31	from	fiber	280:284	arg1	co-products					246:256	The co-products	242:256	The co-products	242:256	The co-products are typically high in fiber and with diverse composition.
36971207	2	31	from	fiber	280:284	arg1	high					272:275	high	272:275	high	272:275	The co-products are typically high in fiber and with diverse composition.
36971207	13	32	theme	energy	2326:2331	arg1	ATTD					2304:2307	low ATTD	2300:2307	low ATTD of nutrients and energy	2300:2331	In contrast, SR and PR show low ATTD of nutrients and energy, thereby compromising the nutritive value.
36971207	7	33	theme	gross	1115:1119	arg1	intake					1133:1138	a gross energy (GE) intake	1113:1138	a gross energy (GE) intake between 28.5 and 42.3 MJ/d	1113:1165	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	10	34	theme	related	1745:1751	arg1	highest					1760:1766	highest	1760:1766	highest	1760:1766	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	10	34	theme	related	1745:1751	arg1	HP					1753:1754	the non-activity related HP	1728:1754	the non-activity related HP	1728:1754	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	11	35	dep	PH	1876:1877	arg1	MJ/d					1901:1904	7.42 and 2.19 MJ/d	1887:1904	7.42 and 2.19 MJ/d	1887:1904	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	8	36	theme	N	1276:1276	arg1	ATTD					1233:1236	The ATTD	1229:1236	The ATTD of dry matter, organic matter, GE and N	1229:1276	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	5	37	theme	pectin	742:747	arg1	PR					758:759	PR	758:759	PR	758:759	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	37	theme	pectin	742:747	arg1	residue					749:755	pectin residue	742:755	pectin residue (PR)	742:760	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	37	theme	pectin	742:747	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	14	38	theme	environmental	2536:2548	arg1	impact					2550:2555	thereby increased environmental impact	2518:2555	thereby increased environmental impact	2518:2555	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	10	39	dep	highest	1760:1766	arg1	sows					1812:1815	fed sows	1808:1815	fed sows (P < 0.05)	1808:1826	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	10	39	dep	highest	1760:1766	arg1	<					1820:1820	P < 0.05	1818:1825	P < 0.05	1818:1825	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	11	40	dep	-0.69	1974:1978	arg1	to					1971:1972	to	1971:1972	to	1971:1972	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	5	41	dep	Brewers	682:688	arg1	pulp					731:734	potato pulp	724:734	potato pulp (PP)	724:739	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	residue					794:800	seed residue	789:800	seed residue (SR)	789:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	residue					749:755	pectin residue	742:755	pectin residue (PR)	742:760	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	grain					696:700	spent grain	690:700	spent grain (BSG)	690:706	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	hull					713:716	pea hull	709:716	pea hull (PH)	709:721	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	SR					803:804	SR	803:804	SR	803:804	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	PR					758:759	PR	758:759	PR	758:759	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	PH					719:720	PH	719:720	PH	719:720	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	SBP					780:782	SBP	780:782	SBP	780:782	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	PP					737:738	PP	737:738	PP	737:738	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	BSG					703:705	BSG	703:705	BSG	703:705	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	41	dep	Brewers	682:688	arg1	pulp					774:777	sugar beet pulp	763:777	sugar beet pulp (SBP)	763:783	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	0	42	from	Influence	0:8	arg1	nutrient					39:46	nutrient	39:46	nutrient	39:46	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	0	42	from	Influence	0:8	arg1	energy					52:57	energy	52:57	energy	52:57	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	6	43	theme	collection	980:989	arg1	period					991:996	The collection period	976:996	The collection period	976:996	The collection period consisted of a total collection period of five days, of which two days were in a respiration chamber.
36971207	1	44	from	industries	141:150	arg1	Co-products					98:108	Co-products	98:108	Co-products from the food and agricultural industries	98:150	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	5	45	theme	basal	825:829	arg1	BD					837:838	BD	837:838	BD	837:838	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	45	theme	basal	825:829	arg1	diet					831:834	a basal diet	823:834	a basal diet (BD)	823:839	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	13	46	theme	nutrients	2312:2320	arg1	ATTD					2304:2307	low ATTD	2300:2307	low ATTD of nutrients and energy	2300:2331	In contrast, SR and PR show low ATTD of nutrients and energy, thereby compromising the nutritive value.
36971207	5	47	theme	pea	709:711	arg1	PH					719:720	PH	719:720	PH	719:720	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	47	theme	pea	709:711	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	47	theme	pea	709:711	arg1	hull					713:716	pea hull	709:716	pea hull (PH)	709:721	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	14	48	theme	compromised	2488:2498	arg1	utilization					2502:2512	compromised N utilization	2488:2512	compromised N utilization	2488:2512	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	12	49	theme	value	2159:2163	arg1	crops					2171:2175	high value grain crops	2154:2175	high value grain crops due to high ATTD of all nutrients	2154:2209	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	8	50	theme	matter	1245:1250	arg1	ATTD					1233:1236	The ATTD	1229:1236	The ATTD of dry matter, organic matter, GE and N	1229:1276	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	8	51	theme	organic	1253:1259	arg1	matter					1261:1266	organic matter	1253:1266	organic matter	1253:1266	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	10	52	theme	fed	1774:1776	arg1	sows					1778:1781	SR fed sows	1771:1781	SR fed sows	1771:1781	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	0	53	theme	fiber-rich	13:22	arg1	co-products					24:34	fiber-rich co-products	13:34	fiber-rich co-products	13:34	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	11	54	dep	intermediate	1922:1933	arg1	greatest					1853:1860	greatest	1853:1860	greatest	1853:1860	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	11	54	dep	intermediate	1922:1933	arg1	MJ/d					2041:2044	-4.26 and -6.17 MJ/d	2025:2044	-4.26 and -6.17 MJ/d	2025:2044	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	11	54	dep	intermediate	1922:1933	arg1	following					1862:1870	following	1862:1870	following the PH and BD (7.42 and 2.19 MJ/d, respectively)	1862:1919	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	11	54	dep	intermediate	1922:1933	arg1	<					2064:2064	P < 0.001	2062:2070	P < 0.001	2062:2070	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	5	55	theme	potato	724:729	arg1	pulp					731:734	potato pulp	724:734	potato pulp (PP)	724:739	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	55	theme	potato	724:729	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	55	theme	potato	724:729	arg1	PP					737:738	PP	737:738	PP	737:738	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	56	theme	square	939:944	arg1	design					968:973	a Youden square incomplete cross-over design	930:973	a Youden square incomplete cross-over design	930:973	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	4	57	theme	apparent	511:518	arg1	ATTD					547:550	ATTD	547:550	ATTD	547:550	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	57	theme	apparent	511:518	arg1	digestibility					532:544	the apparent total tract digestibility	507:544	the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP)	507:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	9	58	theme	P	1653:1653	arg1	PH					1649:1650	PH	1649:1650	PH (P < 0.001)	1649:1662	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	9	58	theme	P	1653:1653	arg1	<					1655:1655	P < 0.001	1653:1661	P < 0.001	1653:1661	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	5	59	theme	cross-over	957:966	arg1	design					968:973	a Youden square incomplete cross-over design	930:973	a Youden square incomplete cross-over design	930:973	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	4	60	theme	tract	526:530	arg1	ATTD					547:550	ATTD	547:550	ATTD	547:550	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	60	theme	tract	526:530	arg1	digestibility					532:544	the apparent total tract digestibility	507:544	the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP)	507:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	8	61	theme	SBP	1321:1323	arg1	sows					1329:1332	the PH and SBP fed sows	1310:1332	sows	1329:1332	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	12	62	contain	have	2117:2120	arg2	potential					2126:2134	the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein	2122:2269	the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein	2122:2269	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	12	62	contain	have	2117:2120	arg1	SBP					2106:2108	SBP	2106:2108	SBP	2106:2108	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	12	62	contain	have	2117:2120	arg1	PH					2114:2115	PH	2114:2115	PH	2114:2115	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	4	63	theme	energy	585:590	arg1	utilization					570:580	utilization	570:580	utilization of energy and nitrogen	570:603	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	63	theme	energy	585:590	arg1	nutrients					556:564	nutrients	556:564	nutrients	556:564	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	11	64	theme	fed	2015:2017	arg1	sows					2019:2022	SR fed sows	2012:2022	SR fed sows	2012:2022	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	7	65	theme	PP	1216:1217	arg1	sows					1223:1226	the PP fed sows	1212:1226	the PP fed sows	1212:1226	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	9	66	theme	digestible	1490:1499	arg1	content					1526:1532	digestible and metabolizable energy content	1490:1532	digestible and metabolizable energy content of the FRCP ingredients	1490:1556	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	4	67	theme	nitrogen	596:603	arg1	utilization					570:580	utilization	570:580	utilization of energy and nitrogen	570:603	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	67	theme	nitrogen	596:603	arg1	nutrients					556:564	nutrients	556:564	nutrients	556:564	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	9	68	theme	metabolizable	1505:1517	arg1	content					1526:1532	digestible and metabolizable energy content	1490:1532	digestible and metabolizable energy content of the FRCP ingredients	1490:1556	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	4	69	theme	nutrients	556:564	arg1	ATTD					547:550	ATTD	547:550	ATTD	547:550	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	69	theme	nutrients	556:564	arg1	digestibility					532:544	the apparent total tract digestibility	507:544	the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP)	507:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	0	70	dep	nutrient	39:46	arg1	utilization					77:87	utilization	77:87	utilization	77:87	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	0	70	dep	nutrient	39:46	arg1	digestibility					59:71	digestibility	59:71	digestibility	59:71	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	7	71	theme	PH	1185:1186	arg1	sows					1192:1195	the PH fed sows	1181:1195	the PH fed sows	1181:1195	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	4	72	theme	utilization	570:580	arg1	ATTD					547:550	ATTD	547:550	ATTD	547:550	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	72	theme	utilization	570:580	arg1	digestibility					532:544	the apparent total tract digestibility	507:544	the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP)	507:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	7	73	theme	fed	1188:1190	arg1	sows					1192:1195	the PH fed sows	1181:1195	the PH fed sows	1181:1195	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	12	74	theme	feeding	2085:2091	arg1	perspective					2093:2103	a sow feeding perspective	2079:2103	a sow feeding perspective	2079:2103	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	10	75	theme	heat	1671:1674	arg1	production					1676:1685	Total heat production	1665:1685	Total heat production (HP)	1665:1690	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	10	75	theme	heat	1671:1674	arg1	HP					1688:1689	HP	1688:1689	HP	1688:1689	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	12	76	theme	nutrients	2201:2209	arg1	ATTD					2189:2192	high ATTD	2184:2192	high ATTD of all nutrients	2184:2209	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	5	77	theme	beet	769:772	arg1	SBP					780:782	SBP	780:782	SBP	780:782	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	77	theme	beet	769:772	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	77	theme	beet	769:772	arg1	pulp					774:777	sugar beet pulp	763:777	sugar beet pulp (SBP)	763:783	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	10	78	theme	fed	1808:1810	arg1	sows					1812:1815	fed sows	1808:1815	fed sows (P < 0.05)	1808:1826	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	10	78	theme	fed	1808:1810	arg1	<					1820:1820	P < 0.05	1818:1825	P < 0.05	1818:1825	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	9	79	theme	ingredients	1546:1556	arg1	content					1526:1532	digestible and metabolizable energy content	1490:1532	digestible and metabolizable energy content of the FRCP ingredients	1490:1556	The differences were caused by variation in digestible and metabolizable energy content of the FRCP ingredients, which was lowest for SR, intermediate for PR followed by BSG and greatest for SBP, PP and PH (P < 0.001).
36971207	4	80	theme	fiber-rich	651:660	arg1	FRCP					675:678	FRCP	675:678	FRCP	675:678	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	80	theme	fiber-rich	651:660	arg1	co-products					662:672	six different fiber-rich co-products	637:672	six different fiber-rich co-products (FRCP)	637:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	10	81	theme	SR	1771:1772	arg1	sows					1778:1781	SR fed sows	1771:1781	SR fed sows	1771:1781	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	1	82	theme	high	200:203	arg1	crops					217:221	concentrated high value grain crops	187:221	concentrated high value grain crops in diets for sows	187:239	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	4	83	theme	empty	608:612	arg1	sows					628:631	empty non-lactating sows	608:631	empty non-lactating sows fed six different fiber-rich co-products (FRCP)	608:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	1	84	theme	grain	211:215	arg1	crops					217:221	concentrated high value grain crops	187:221	concentrated high value grain crops in diets for sows	187:239	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	3	85	from	high	367:370	arg1	sows					375:378	sows	375:378	sows fed fiber-rich feedstuff	375:403	Energy digestibility and utilization are generally high in sows fed fiber-rich feedstuff, but nitrogen digestion and utilization may be compromised.
36971207	1	86	from	crops	217:221	arg1	diets					226:230	diets	226:230	diets for sows	226:239	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	5	87	theme	seed	789:792	arg1	residue					794:800	seed residue	789:800	seed residue (SR)	789:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	87	theme	seed	789:792	arg1	Brewers					682:688	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR)	682:805	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	87	theme	seed	789:792	arg1	SR					803:804	SR	803:804	SR	803:804	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	13	88	theme	nutritive	2359:2367	arg1	value					2369:2373	the nutritive value	2355:2373	the nutritive value	2355:2373	In contrast, SR and PR show low ATTD of nutrients and energy, thereby compromising the nutritive value.
36971207	6	89	theme	collection	1019:1028	arg1	period					1030:1035	a total collection period	1011:1035	a total collection period	1011:1035	The collection period consisted of a total collection period of five days, of which two days were in a respiration chamber.
36971207	6	90	theme	respiration	1079:1089	arg1	chamber					1091:1097	a respiration chamber	1077:1097	a respiration chamber	1077:1097	The collection period consisted of a total collection period of five days, of which two days were in a respiration chamber.
36971207	2	91	with	high	272:275	arg1	composition					303:313	diverse composition	295:313	diverse composition	295:313	The co-products are typically high in fiber and with diverse composition.
36971207	7	92	theme	GE	1129:1130	arg1	intake					1133:1138	a gross energy (GE) intake	1113:1138	a gross energy (GE) intake between 28.5 and 42.3 MJ/d	1113:1165	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	7	93	contain	had	1109:1111	arg1	sows					1104:1107	The sows	1100:1107	The sows	1100:1107	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	7	93	contain	had	1109:1111	arg2	intake					1133:1138	a gross energy (GE) intake	1113:1138	a gross energy (GE) intake between 28.5 and 42.3 MJ/d	1113:1165	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	8	94	theme	SR	1422:1423	arg1	sows					1429:1432	SR fed sows	1422:1432	SR fed sows (P < 0.01)	1422:1443	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	8	94	theme	SR	1422:1423	arg1	<					1437:1437	P < 0.01	1435:1442	P < 0.01	1435:1442	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	7	95	theme	energy	1121:1126	arg1	intake					1133:1138	a gross energy (GE) intake	1113:1138	a gross energy (GE) intake between 28.5 and 42.3 MJ/d	1113:1165	The sows had a gross energy (GE) intake between 28.5 and 42.3 MJ/d; greatest for the PH fed sows and lowest for the PP fed sows.
36971207	10	96	theme	non-activity	1732:1743	arg1	highest					1760:1766	highest	1760:1766	highest	1760:1766	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	10	96	theme	non-activity	1732:1743	arg1	HP					1753:1754	the non-activity related HP	1728:1754	the non-activity related HP	1728:1754	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	14	97	theme	Potato	2376:2381	arg1	pulp					2383:2386	Potato pulp	2376:2386	Potato pulp	2376:2386	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	14	98	theme	increased	2526:2534	arg1	impact					2550:2555	thereby increased environmental impact	2518:2555	thereby increased environmental impact	2518:2555	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	11	99	theme	fed	1955:1957	arg1	MJ/d					1980:1983	-0.22 to -0.69 MJ/d	1965:1983	-0.22 to -0.69 MJ/d	1965:1983	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	11	99	theme	fed	1955:1957	arg1	sows					1959:1962	BSG fed sows	1951:1962	BSG fed sows (-0.22 to -0.69 MJ/d)	1951:1984	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36971207	13	100	theme	low	2300:2302	arg1	ATTD					2304:2307	low ATTD	2300:2307	low ATTD of nutrients and energy	2300:2331	In contrast, SR and PR show low ATTD of nutrients and energy, thereby compromising the nutritive value.
36971207	3	101	theme	Energy	316:321	arg1	digestibility					323:335	Energy digestibility	316:335	Energy digestibility	316:335	Energy digestibility and utilization are generally high in sows fed fiber-rich feedstuff, but nitrogen digestion and utilization may be compromised.
36971207	8	102	dep	PR	1401:1402	arg1	lowest					1412:1417	lowest	1412:1417	lowest	1412:1417	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	1	103	used	used	171:174	arg2	Co-products					98:108	Co-products	98:108	Co-products from the food and agricultural industries	98:150	Co-products from the food and agricultural industries can potentially be used to replace concentrated high value grain crops in diets for sows.
36971207	5	104	theme	Youden	932:937	arg1	design					968:973	a Youden square incomplete cross-over design	930:973	a Youden square incomplete cross-over design	930:973	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	8	105	theme	P	1435:1435	arg1	sows					1429:1432	SR fed sows	1422:1432	SR fed sows (P < 0.01)	1422:1443	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	8	105	theme	P	1435:1435	arg1	<					1437:1437	P < 0.01	1435:1442	P < 0.01	1435:1442	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	12	106	theme	due	2177:2179	arg1	crops					2171:2175	high value grain crops	2154:2175	high value grain crops due to high ATTD of all nutrients	2154:2209	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	8	107	theme	dry	1241:1243	arg1	matter					1245:1250	dry matter	1241:1250	dry matter	1241:1250	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	10	108	theme	Total	1665:1669	arg1	production					1676:1685	Total heat production	1665:1685	Total heat production (HP)	1665:1690	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	10	108	theme	Total	1665:1669	arg1	HP					1688:1689	HP	1688:1689	HP	1688:1689	Total heat production (HP) did not differ among treatments but the non-activity related HP was highest in SR fed sows and lowest in PH and SBP fed sows (P < 0.05).
36971207	3	109	from	sows	375:378	arg1	high					367:370	high	367:370	high	367:370	Energy digestibility and utilization are generally high in sows fed fiber-rich feedstuff, but nitrogen digestion and utilization may be compromised.
36971207	14	110	theme	N	2500:2500	arg1	utilization					2502:2512	compromised N utilization	2488:2512	compromised N utilization	2488:2512	Potato pulp and BSG also have the potential to be included in sow diets, but caution should be taken because of compromised N utilization and thereby increased environmental impact.
36971207	12	111	theme	high	2184:2187	arg1	ATTD					2189:2192	high ATTD	2184:2192	high ATTD of all nutrients	2184:2209	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	4	112	theme	study	485:489	arg1	purpose					469:475	The purpose	465:475	The purpose of this study	465:489	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	5	113	theme	empty	916:920	arg1	sows					922:925	8 empty sows	914:925	8 empty sows in a Youden square incomplete cross-over design	914:973	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	3	114	theme	fiber-rich	384:393	arg1	feedstuff					395:403	fiber-rich feedstuff	384:403	fiber-rich feedstuff	384:403	Energy digestibility and utilization are generally high in sows fed fiber-rich feedstuff, but nitrogen digestion and utilization may be compromised.
36971207	8	115	theme	matter	1261:1266	arg1	ATTD					1233:1236	The ATTD	1229:1236	The ATTD of dry matter, organic matter, GE and N	1229:1276	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	0	116	theme	co-products	24:34	arg1	Influence					0:8	Influence	0:8	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.	0:96	Influence of fiber-rich co-products on nutrient and energy digestibility and utilization in sows.
36971207	12	117	theme	grain	2165:2169	arg1	crops					2171:2175	high value grain crops	2154:2175	high value grain crops due to high ATTD of all nutrients	2154:2209	From a sow feeding perspective, SBP and PH have the potential to partly replace high value grain crops due to high ATTD of all nutrients and because sows can efficiently utilize energy and protein.
36971207	8	118	theme	GE	1269:1270	arg1	ATTD					1233:1236	The ATTD	1229:1236	The ATTD of dry matter, organic matter, GE and N	1229:1276	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	4	119	from	digestibility	532:544	arg1	sows					628:631	empty non-lactating sows	608:631	empty non-lactating sows fed six different fiber-rich co-products (FRCP)	608:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	5	120	theme	high	849:852	arg1	level					867:871	an inclusion level	854:871	as high an inclusion level	846:871	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	3	121	theme	nitrogen	410:417	arg1	digestion					419:427	nitrogen digestion	410:427	nitrogen digestion	410:427	Energy digestibility and utilization are generally high in sows fed fiber-rich feedstuff, but nitrogen digestion and utilization may be compromised.
36971207	4	122	theme	total	520:524	arg1	ATTD					547:550	ATTD	547:550	ATTD	547:550	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	4	122	theme	total	520:524	arg1	digestibility					532:544	the apparent total tract digestibility	507:544	the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP)	507:679	The purpose of this study was to quantify the apparent total tract digestibility (ATTD) of nutrients and utilization of energy and nitrogen in empty non-lactating sows fed six different fiber-rich co-products (FRCP).
36971207	5	123	from	sows	922:925	arg1	design					968:973	a Youden square incomplete cross-over design	930:973	a Youden square incomplete cross-over design	930:973	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	124	theme	incomplete	946:955	arg1	design					968:973	a Youden square incomplete cross-over design	930:973	a Youden square incomplete cross-over design	930:973	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	5	125	theme	inclusion	857:865	arg1	level					867:871	an inclusion level	854:871	as high an inclusion level	846:871	Brewers spent grain (BSG), pea hull (PH), potato pulp (PP), pectin residue (PR), sugar beet pulp (SBP) and seed residue (SR) were mixed into a basal diet (BD) with as high an inclusion level as possible, or the BD was fed solely to 8 empty sows in a Youden square incomplete cross-over design.
36971207	8	126	theme	fed	1325:1327	arg1	sows					1329:1332	the PH and SBP fed sows	1310:1332	sows	1329:1332	The ATTD of dry matter, organic matter, GE and N did not differ among the BD and the PH and SBP fed sows, while the ATTDs of all nutrients and energy were intermediate for PR and BSG lowest in SR fed sows (P < 0.01).
36971207	11	127	theme	energy	1842:1847	arg1	Retention					1829:1837	Retention	1829:1837	Retention of energy	1829:1847	Retention of energy was greatest following the PH and BD (7.42 and 2.19 MJ/d, respectively), intermediate for PP, SBP and BSG fed sows (-0.22 to -0.69 MJ/d) and lowest for the PR and SR fed sows (-4.26 and -6.17 MJ/d, respectively) (P < 0.001).
36731153	8	0	theme	colitis	1005:1011	arg1	DAI					997:999	DAI	997:999	DAI	997:999	Then, the disease activity index (DAI) of colitis was analyzed.
36731153	8	0	theme	colitis	1005:1011	arg1	index					990:994	the disease activity index	969:994	the disease activity index (DAI) of colitis	969:1011	Then, the disease activity index (DAI) of colitis was analyzed.
36731153	15	1	theme	potent	1763:1768	arg1	activity					1780:1787	potent antitumor activity	1763:1787	potent antitumor activity against colorectal cancer	1763:1813	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	12	2	theme	AQB	1355:1357	arg1	treatment					1359:1367	AQB treatment	1355:1367	AQB treatment	1355:1367	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	2	3	theme	BACKGROUND	228:237	arg1	malignancy					275:284	a common malignancy	266:284	a common malignancy with high mortality and few effective therapeutic measures	266:343	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	2	3	theme	BACKGROUND	228:237	arg1	CRC					258:260	CRC	258:260	CRC	258:260	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	2	3	theme	BACKGROUND	228:237	arg1	cancer					250:255	BACKGROUND Colorectal cancer	228:255	BACKGROUND Colorectal cancer (CRC)	228:261	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	13	4	theme	CD4++CD25++Foxp3++Regulatory	1504:1531	arg1	Tregs					1542:1546	Tregs	1542:1546	Tregs	1542:1546	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	13	4	theme	CD4++CD25++Foxp3++Regulatory	1504:1531	arg1	cells					1535:1539	CD4++CD25++Foxp3++Regulatory T cells	1504:1539	CD4++CD25++Foxp3++Regulatory T cells (Tregs)	1504:1547	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	14	5	theme	microbiota	1617:1626	arg1	structure					1580:1588	structure	1580:1588	structure	1580:1588	AQB regulated the structure and composition of the gut microbiota, which decreased the Firmicutes/Bacteroidetes ratio at the phylum level and increased the abundance of probiotics.
36731153	14	5	theme	microbiota	1617:1626	arg1	composition					1594:1604	composition	1594:1604	composition	1594:1604	AQB regulated the structure and composition of the gut microbiota, which decreased the Firmicutes/Bacteroidetes ratio at the phylum level and increased the abundance of probiotics.
36731153	5	6	theme	10 mg/kg	620:627	arg1	injection					607:615	intraperitoneal injection	591:615	intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS)	591:686	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	13	7	theme	cells	1535:1539	arg1	accumulation					1488:1499	the accumulation	1484:1499	the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells	1484:1559	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	12	8	theme	CRC	1338:1340	arg1	model					1348:1352	AOM/DSS-induced CRC mouse model	1322:1352	AOM/DSS-induced CRC mouse model	1322:1352	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	6	9	dep	groups	743:748	arg1	groups					743:748	four groups	738:748	four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB)	738:846	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	AOM/DSS					772:778	AOM/DSS	772:778	AOM/DSS (model control, MC)	772:798	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	DMSO + AOM/DSS					801:814	DMSO + AOM/DSS	801:814	DMSO + AOM/DSS (DMSO)	801:821	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	control					758:764	normal control	751:764	normal control (NC)	751:769	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	AQB + AOM/DSS					828:840	AQB + AOM/DSS	828:840	AQB + AOM/DSS (AQB)	828:846	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	NC					767:768	NC	767:768	NC	767:768	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	AQB					843:845	AQB	843:845	AQB	843:845	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	control					787:793	model control	781:793	model control	781:793	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	9	dep	groups	743:748	arg1	DMSO					817:820	DMSO	817:820	DMSO	817:820	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	5	10	theme	Azoxymethane	632:643	arg1	10 mg/kg					620:627	10 mg/kg	620:627	10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS)	620:686	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	0	11	theme	intestinal	117:126	arg1	microbiota					128:137	the intestinal microbiota	113:137	the intestinal microbiota	113:137	AC1Q3QWB inhibits colorectal cancer progression by modulating the immune response and balancing the structure of the intestinal microbiota.
36731153	14	12	theme	gut	1613:1615	arg1	microbiota					1617:1626	the gut microbiota	1609:1626	the gut microbiota	1609:1626	AQB regulated the structure and composition of the gut microbiota, which decreased the Firmicutes/Bacteroidetes ratio at the phylum level and increased the abundance of probiotics.
36731153	2	13	theme	high	291:294	arg1	mortality					296:304	high mortality	291:304	high mortality	291:304	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	11	14	theme	flow	1254:1257	arg1	cytometry					1259:1267	flow cytometry	1254:1267	flow cytometry	1254:1267	Blood samples were analyzed by flow cytometry to investigate the inflammatory response.
36731153	2	15	theme	Colorectal	239:248	arg1	malignancy					275:284	a common malignancy	266:284	a common malignancy with high mortality and few effective therapeutic measures	266:343	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	2	15	theme	Colorectal	239:248	arg1	CRC					258:260	CRC	258:260	CRC	258:260	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	2	15	theme	Colorectal	239:248	arg1	cancer					250:255	BACKGROUND Colorectal cancer	228:255	BACKGROUND Colorectal cancer (CRC)	228:261	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	7	16	theme	intraperitoneal	892:906	arg1	injection					908:916	an intraperitoneal injection	889:916	an intraperitoneal injection of AQB (50 mg/kg) after successful modeling	889:960	Mice in the AQB group were treated with an intraperitoneal injection of AQB (50 mg/kg) after successful modeling.
36731153	1	17	theme	cells	221:225	arg1	accumulation					193:204	accumulation	193:204	accumulation of Tregs and B cells	193:225	AC1Q3QWB enhances CD8 + T cell response and triggers accumulation of Tregs and B cells.
36731153	15	18	theme	colorectal	1797:1806	arg1	cancer					1808:1813	colorectal cancer	1797:1813	colorectal cancer	1797:1813	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	5	19	theme	%	657:657	arg1	DSS					683:685	DSS	683:685	DSS	683:685	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	5	19	theme	%	657:657	arg1	sulfate					674:680	2 % dextran sodium sulfate	655:680	2 % dextran sodium sulfate (DSS)	655:686	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	12	20	dep	number	1394:1399	arg1	the					1390:1392	the	1390:1392	the	1390:1392	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	7	21	theme	AQB	921:923	arg1	injection					908:916	an intraperitoneal injection	889:916	an intraperitoneal injection of AQB (50 mg/kg) after successful modeling	889:960	Mice in the AQB group were treated with an intraperitoneal injection of AQB (50 mg/kg) after successful modeling.
36731153	0	22	theme	immune	66:71	arg1	response					73:80	the immune response	62:80	the immune response	62:80	AC1Q3QWB inhibits colorectal cancer progression by modulating the immune response and balancing the structure of the intestinal microbiota.
36731153	7	23	theme	AQB	861:863	arg1	group					865:869	the AQB group	857:869	the AQB group	857:869	Mice in the AQB group were treated with an intraperitoneal injection of AQB (50 mg/kg) after successful modeling.
36731153	1	24	theme	CD8 + T	158:164	arg1	response					171:178	CD8 + T cell response	158:178	CD8 + T cell response	158:178	AC1Q3QWB enhances CD8 + T cell response and triggers accumulation of Tregs and B cells.
36731153	2	25	with	malignancy	275:284	arg1	measures					336:343	few effective therapeutic measures	310:343	few effective therapeutic measures	310:343	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	2	25	with	malignancy	275:284	arg1	mortality					296:304	high mortality	291:304	high mortality	291:304	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	15	26	theme	immune	1875:1880	arg1	system					1882:1887	the immune system	1871:1887	the immune system	1871:1887	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	5	27	theme	sodium	667:672	arg1	DSS					683:685	DSS	683:685	DSS	683:685	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	5	27	theme	sodium	667:672	arg1	sulfate					674:680	2 % dextran sodium sulfate	655:680	2 % dextran sodium sulfate (DSS)	655:686	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	10	28	theme	Stool	1146:1150	arg1	samples					1152:1158	Stool samples	1146:1158	Stool samples	1146:1158	Stool samples were collected for microbiota analysis by 16S rRNA sequencing.
36731153	14	29	theme	probiotics	1731:1740	arg1	abundance					1718:1726	the abundance	1714:1726	the abundance of probiotics	1714:1740	AQB regulated the structure and composition of the gut microbiota, which decreased the Firmicutes/Bacteroidetes ratio at the phylum level and increased the abundance of probiotics.
36731153	12	30	theme	tumors	1419:1424	arg1	size					1405:1408	size	1405:1408	size	1405:1408	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	12	30	theme	tumors	1419:1424	arg1	number					1394:1399	number	1394:1399	number	1394:1399	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	5	31	theme	intraperitoneal	591:605	arg1	injection					607:615	intraperitoneal injection	591:615	intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS)	591:686	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	3	32	theme	Gut	346:348	arg1	dysbiosis					361:369	Gut microbiota dysbiosis	346:369	Gut microbiota dysbiosis	346:369	Gut microbiota dysbiosis and chronic inflammation might contribute to the development of CRC.
36731153	5	33	theme	colon	553:557	arg1	model					566:570	A mouse colon cancer model	545:570	A mouse colon cancer model	545:570	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	3	34	theme	CRC	435:437	arg1	development					420:430	the development	416:430	the development of CRC	416:437	Gut microbiota dysbiosis and chronic inflammation might contribute to the development of CRC.
36731153	3	35	theme	chronic	375:381	arg1	inflammation					383:394	chronic inflammation	375:394	chronic inflammation	375:394	Gut microbiota dysbiosis and chronic inflammation might contribute to the development of CRC.
36731153	13	36	theme	B	1553:1553	arg1	cells					1555:1559	B cells	1553:1559	B cells	1553:1559	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	0	37	theme	colorectal	18:27	arg1	progression					36:46	colorectal cancer progression	18:46	colorectal cancer progression	18:46	AC1Q3QWB inhibits colorectal cancer progression by modulating the immune response and balancing the structure of the intestinal microbiota.
36731153	13	38	theme	T	1533:1533	arg1	Tregs					1542:1546	Tregs	1542:1546	Tregs	1542:1546	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	13	38	theme	T	1533:1533	arg1	cells					1535:1539	CD4++CD25++Foxp3++Regulatory T cells	1504:1539	CD4++CD25++Foxp3++Regulatory T cells (Tregs)	1504:1547	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	15	39	theme	intestinal	1911:1920	arg1	microbiota					1922:1931	the intestinal microbiota	1907:1931	the intestinal microbiota	1907:1931	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	12	40	theme	colon	1413:1417	arg1	tumors					1419:1424	colon tumors	1413:1424	colon tumors	1413:1424	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	4	41	theme	colon	507:511	arg1	carcinogenesis					513:526	colon carcinogenesis	507:526	colon carcinogenesis	507:526	The present study aimed to explore the effect of AC1Q3QWB (AQB) on colon carcinogenesis in vivo.
36731153	14	42	dep	structure	1580:1588	arg1	the					1576:1578	the	1576:1578	the	1576:1578	AQB regulated the structure and composition of the gut microbiota, which decreased the Firmicutes/Bacteroidetes ratio at the phylum level and increased the abundance of probiotics.
36731153	14	43	theme	phylum	1687:1692	arg1	level					1694:1698	the phylum level	1683:1698	the phylum level	1683:1698	AQB regulated the structure and composition of the gut microbiota, which decreased the Firmicutes/Bacteroidetes ratio at the phylum level and increased the abundance of probiotics.
36731153	8	44	theme	disease	973:979	arg1	DAI					997:999	DAI	997:999	DAI	997:999	Then, the disease activity index (DAI) of colitis was analyzed.
36731153	8	44	theme	disease	973:979	arg1	index					990:994	the disease activity index	969:994	the disease activity index (DAI) of colitis	969:1011	Then, the disease activity index (DAI) of colitis was analyzed.
36731153	2	45	theme	therapeutic	324:334	arg1	measures					336:343	few effective therapeutic measures	310:343	few effective therapeutic measures	310:343	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	15	46	theme	antitumor	1770:1778	arg1	activity					1780:1787	potent antitumor activity	1763:1787	potent antitumor activity against colorectal cancer	1763:1813	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	12	47	dep	RESULTS	1311:1317	arg1	reduced					1382:1388	reduced	1382:1388	reduced the number and size of colon tumors	1382:1424	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	14	48	theme	Firmicutes/Bacteroidetes	1649:1672	arg1	ratio					1674:1678	the Firmicutes/Bacteroidetes ratio	1645:1678	the Firmicutes/Bacteroidetes ratio	1645:1678	AQB regulated the structure and composition of the gut microbiota, which decreased the Firmicutes/Bacteroidetes ratio at the phylum level and increased the abundance of probiotics.
36731153	9	49	theme	Colon	1027:1031	arg1	tissues					1033:1039	Colon tissues	1027:1039	Colon tissues	1027:1039	Colon tissues were collected for hematoxylin-eosin, immunohistochemistry, and microscopic and histological evaluation.
36731153	12	50	theme	AOM/DSS-induced	1322:1336	arg1	model					1348:1352	AOM/DSS-induced CRC mouse model	1322:1352	AOM/DSS-induced CRC mouse model	1322:1352	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	5	51	dep	METHODS	537:543	arg1	constructed					576:586	constructed	576:586	was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water	572:704	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	11	52	theme	inflammatory	1288:1299	arg1	response					1301:1308	the inflammatory response	1284:1308	the inflammatory response	1284:1308	Blood samples were analyzed by flow cytometry to investigate the inflammatory response.
36731153	6	53	theme	normal	751:756	arg1	groups					743:748	four groups	738:748	four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB)	738:846	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	53	theme	normal	751:756	arg1	control					758:764	normal control	751:764	normal control (NC)	751:769	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	53	theme	normal	751:756	arg1	NC					767:768	NC	767:768	NC	767:768	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	2	54	theme	common	268:273	arg1	malignancy					275:284	a common malignancy	266:284	a common malignancy with high mortality and few effective therapeutic measures	266:343	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	2	54	theme	common	268:273	arg1	cancer					250:255	BACKGROUND Colorectal cancer	228:255	BACKGROUND Colorectal cancer (CRC)	228:261	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	12	55	theme	mouse	1342:1346	arg1	model					1348:1352	AOM/DSS-induced CRC mouse model	1322:1352	AOM/DSS-induced CRC mouse model	1322:1352	RESULTS In AOM/DSS-induced CRC mouse model, AQB treatment dramatically reduced the number and size of colon tumors.
36731153	11	56	theme	Blood	1223:1227	arg1	samples					1229:1235	Blood samples	1223:1235	Blood samples	1223:1235	Blood samples were analyzed by flow cytometry to investigate the inflammatory response.
36731153	4	57	from	effect	479:484	arg1	carcinogenesis					513:526	colon carcinogenesis	507:526	colon carcinogenesis	507:526	The present study aimed to explore the effect of AC1Q3QWB (AQB) on colon carcinogenesis in vivo.
36731153	2	58	theme	few	310:312	arg1	measures					336:343	few effective therapeutic measures	310:343	few effective therapeutic measures	310:343	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
36731153	7	59	theme	successful	942:951	arg1	modeling					953:960	successful modeling	942:960	successful modeling	942:960	Mice in the AQB group were treated with an intraperitoneal injection of AQB (50 mg/kg) after successful modeling.
36731153	1	60	theme	Tregs	209:213	arg1	accumulation					193:204	accumulation	193:204	accumulation of Tregs and B cells	193:225	AC1Q3QWB enhances CD8 + T cell response and triggers accumulation of Tregs and B cells.
36731153	9	61	theme	microscopic	1105:1115	arg1	evaluation					1134:1143	microscopic and histological evaluation	1105:1143	microscopic and histological evaluation	1105:1143	Colon tissues were collected for hematoxylin-eosin, immunohistochemistry, and microscopic and histological evaluation.
36731153	1	62	theme	B	219:219	arg1	cells					221:225	B cells	219:225	B cells	219:225	AC1Q3QWB enhances CD8 + T cell response and triggers accumulation of Tregs and B cells.
36731153	5	63	theme	2 	655:656	arg1	%					657:657	%	657:657	%	657:657	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	9	64	theme	histological	1121:1132	arg1	evaluation					1134:1143	microscopic and histological evaluation	1105:1143	microscopic and histological evaluation	1105:1143	Colon tissues were collected for hematoxylin-eosin, immunohistochemistry, and microscopic and histological evaluation.
36731153	13	65	theme	cell	1457:1460	arg1	response					1462:1469	CD8++T cell response	1450:1469	CD8++T cell response	1450:1469	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	0	66	theme	microbiota	128:137	arg1	structure					100:108	the structure	96:108	the structure of the intestinal microbiota	96:137	AC1Q3QWB inhibits colorectal cancer progression by modulating the immune response and balancing the structure of the intestinal microbiota.
36731153	5	67	theme	dextran	659:665	arg1	DSS					683:685	DSS	683:685	DSS	683:685	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	5	67	theme	dextran	659:665	arg1	sulfate					674:680	2 % dextran sodium sulfate	655:680	2 % dextran sodium sulfate (DSS)	655:686	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	5	68	theme	mouse	547:551	arg1	model					566:570	A mouse colon cancer model	545:570	A mouse colon cancer model	545:570	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	4	69	theme	present	444:450	arg1	study					452:456	The present study	440:456	The present study	440:456	The present study aimed to explore the effect of AC1Q3QWB (AQB) on colon carcinogenesis in vivo.
36731153	13	70	theme	AQB	1427:1429	arg1	treatment					1431:1439	AQB treatment	1427:1439	AQB treatment	1427:1439	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	5	71	theme	sulfate	674:680	arg1	10 mg/kg					620:627	10 mg/kg	620:627	10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS)	620:686	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	7	72	from	Mice	849:852	arg1	group					865:869	the AQB group	857:869	the AQB group	857:869	Mice in the AQB group were treated with an intraperitoneal injection of AQB (50 mg/kg) after successful modeling.
36731153	1	73	theme	cell	166:169	arg1	response					171:178	CD8 + T cell response	158:178	CD8 + T cell response	158:178	AC1Q3QWB enhances CD8 + T cell response and triggers accumulation of Tregs and B cells.
36731153	15	74	theme	system	1882:1887	arg1	modulation					1857:1866	modulation	1857:1866	modulation of the immune system	1857:1887	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	15	74	theme	system	1882:1887	arg1	alteration					1893:1902	alteration	1893:1902	alteration of the intestinal microbiota	1893:1931	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	10	75	theme	microbiota	1179:1188	arg1	analysis					1190:1197	microbiota analysis	1179:1197	microbiota analysis	1179:1197	Stool samples were collected for microbiota analysis by 16S rRNA sequencing.
36731153	3	76	theme	microbiota	350:359	arg1	dysbiosis					361:369	Gut microbiota dysbiosis	346:369	Gut microbiota dysbiosis	346:369	Gut microbiota dysbiosis and chronic inflammation might contribute to the development of CRC.
36731153	4	77	theme	AC1Q3QWB	489:496	arg1	effect					479:484	the effect	475:484	the effect of AC1Q3QWB (AQB) on colon carcinogenesis	475:526	The present study aimed to explore the effect of AC1Q3QWB (AQB) on colon carcinogenesis in vivo.
36731153	5	78	theme	cancer	559:564	arg1	model					566:570	A mouse colon cancer model	545:570	A mouse colon cancer model	545:570	METHODS A mouse colon cancer model was constructed by intraperitoneal injection of 10 mg/kg of Azoxymethane (AOM) and 2 % dextran sodium sulfate (DSS) in drinking water.
36731153	10	79	theme	rRNA	1206:1209	arg1	sequencing					1211:1220	rRNA sequencing	1206:1220	rRNA sequencing	1206:1220	Stool samples were collected for microbiota analysis by 16S rRNA sequencing.
36731153	0	80	theme	cancer	29:34	arg1	progression					36:46	colorectal cancer progression	18:46	colorectal cancer progression	18:46	AC1Q3QWB inhibits colorectal cancer progression by modulating the immune response and balancing the structure of the intestinal microbiota.
36731153	13	81	theme	cells	1555:1559	arg1	accumulation					1488:1499	the accumulation	1484:1499	the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells	1484:1559	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	6	82	dep	control	787:793	arg1	MC					796:797	MC	796:797	MC	796:797	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	15	83	theme	microbiota	1922:1931	arg1	modulation					1857:1866	modulation	1857:1866	modulation of the immune system	1857:1887	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	15	83	theme	microbiota	1922:1931	arg1	alteration					1893:1902	alteration	1893:1902	alteration of the intestinal microbiota	1893:1931	CONCLUSIONS AQB has potent antitumor activity against colorectal cancer in vivo by a mechanism that might involve modulation of the immune system and alteration of the intestinal microbiota.
36731153	8	84	theme	activity	981:988	arg1	DAI					997:999	DAI	997:999	DAI	997:999	Then, the disease activity index (DAI) of colitis was analyzed.
36731153	8	84	theme	activity	981:988	arg1	index					990:994	the disease activity index	969:994	the disease activity index (DAI) of colitis	969:1011	Then, the disease activity index (DAI) of colitis was analyzed.
36731153	6	85	theme	model	781:785	arg1	AOM/DSS					772:778	AOM/DSS	772:778	AOM/DSS (model control, MC)	772:798	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	6	85	theme	model	781:785	arg1	control					787:793	model control	781:793	model control	781:793	Mice were randomly assigned to four groups: normal control (NC), AOM/DSS (model control, MC), DMSO + AOM/DSS (DMSO), and AQB + AOM/DSS (AQB).
36731153	13	86	theme	CD8++T	1450:1455	arg1	response					1462:1469	CD8++T cell response	1450:1469	CD8++T cell response	1450:1469	AQB treatment enhances CD8++T cell response and triggers the accumulation of CD4++CD25++Foxp3++Regulatory T cells (Tregs) and B cells.
36731153	2	87	theme	effective	314:322	arg1	measures					336:343	few effective therapeutic measures	310:343	few effective therapeutic measures	310:343	BACKGROUND Colorectal cancer (CRC) is a common malignancy with high mortality and few effective therapeutic measures.
37203220	4	0	theme	appreciable	1064:1074	arg1	repairment					1083:1092	appreciable tissue repairment	1064:1092	appreciable tissue repairment	1064:1092	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	6	1	theme	mAb-treated	1539:1549	arg1	mice					1561:1564	anti-TNF-α mAb-treated recipient mice	1528:1564	anti-TNF-α mAb-treated recipient mice	1528:1564	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	1	2	theme	complex	250:256	arg1	metabolites					258:268	complex metabolites	250:268	complex metabolites	250:268	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	2	3	theme	aberrant	614:621	arg1	metabolism					629:638	aberrant lipid metabolism	614:638	aberrant lipid metabolism	614:638	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	5	4	dep	composition	1365:1375	arg1	the					1361:1363	the	1361:1363	the	1361:1363	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	6	5	with	co-administration	1571:1587	arg1	muciniphila					1613:1623	Akkermansia muciniphila	1601:1623	Akkermansia muciniphila	1601:1623	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	6	6	theme	significant	1632:1642	arg1	protections					1656:1666	significant synergistic protections	1632:1666	significant synergistic protections against colitis in mice	1632:1690	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	7	7	theme	intestinal	1882:1891	arg1	homeostasis					1893:1903	the intestinal homeostasis	1878:1903	the intestinal homeostasis	1878:1903	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	2	8	theme	unresolved	558:567	arg1	inflammation					569:580	extensive unresolved inflammation	548:580	extensive unresolved inflammation	548:580	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	3	9	theme	cell	758:761	arg1	infiltrations					763:775	inflammatory cell infiltrations	745:775	inflammatory cell infiltrations	745:775	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	6	10	theme	Oral	1411:1414	arg1	transfer					1416:1423	Oral transfer	1411:1423	Oral transfer of such POA-reprogrammed, but not control, gut microbiota	1411:1481	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	7	11	theme	critical	1738:1745	arg1	importance					1747:1756	the critical importance	1734:1756	the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis	1734:1903	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	4	12	theme	inflamed	939:946	arg1	tissues					954:960	cultured inflamed colon tissues	930:960	cultured inflamed colon tissues derived from Crohn's disease (CD) patients	930:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	5	13	from	growth	1269:1274	arg1	microbiota					1324:1333	gut microbiota	1320:1333	gut microbiota	1320:1333	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	1	14	theme	gut	127:129	arg1	microbiota					131:140	gut microbiota	127:140	gut microbiota	127:140	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	7	15	theme	microbiota	1845:1854	arg1	diversity					1828:1836	diversity	1828:1836	diversity	1828:1836	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	7	15	theme	microbiota	1845:1854	arg1	magnitude					1814:1822	magnitude	1814:1822	magnitude	1814:1822	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	2	16	theme	mucosal	590:596	arg1	repairment					598:607	failed mucosal repairment	583:607	failed mucosal repairment	583:607	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	7	17	theme	polyfunctional	1770:1783	arg1	force					1795:1799	a polyfunctional molecular force	1768:1799	a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis	1768:1903	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	0	18	theme	biological	64:73	arg1	therapy					75:81	biological therapy	64:81	biological therapy against colitis	64:97	Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis.
37203220	1	19	theme	systems	156:162	arg1	Magnitude					100:108	Magnitude	100:108	Magnitude	100:108	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	1	19	theme	systems	156:162	arg1	diversity					114:122	diversity	114:122	diversity	114:122	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	2	20	theme	intestinal	485:494	arg1	dysbacteriosis					496:509	intestinal dysbacteriosis	485:509	intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA)	485:691	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	3	21	theme	therapy	848:854	arg1	efficacy					825:832	efficacy	825:832	efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models	825:897	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	4	22	dep	Ex	900:901	arg1	vivo					903:906	vivo	903:906	vivo	903:906	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	5	23	theme	division	1181:1188	arg1	signatures					1162:1171	the transcriptional signatures	1142:1171	the transcriptional signatures of cell division	1142:1188	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	5	23	theme	division	1181:1188	arg1	process					1207:1213	biosynthetic process	1194:1213	biosynthetic process of Akkermansia muciniphila	1194:1240	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	3	24	theme	IBD	882:884	arg1	models					892:897	both acute and chronic IBD mouse models	859:897	both acute and chronic IBD mouse models	859:897	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	2	25	theme	acid	682:685	arg1	inflammation					569:580	extensive unresolved inflammation	548:580	extensive unresolved inflammation	548:580	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	25	theme	acid	682:685	arg1	repairment					598:607	failed mucosal repairment	583:607	failed mucosal repairment	583:607	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	25	theme	acid	682:685	arg1	metabolism					629:638	aberrant lipid metabolism	614:638	aberrant lipid metabolism	614:638	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	25	theme	acid	682:685	arg1	bacteria					538:545	more pro-inflammatory bacteria	516:545	more pro-inflammatory bacteria	516:545	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	25	theme	acid	682:685	arg1	levels					660:665	lower levels	654:665	lower levels of palmitoleic acid (POA)	654:691	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	6	26	theme	POA-reprogrammed	1433:1448	arg1	microbiota					1472:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	2	27	theme	pro-inflammatory	521:536	arg1	bacteria					538:545	more pro-inflammatory bacteria	516:545	more pro-inflammatory bacteria	516:545	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	28	dep	failures	364:371	arg1	patients					455:462	patients	455:462	patients	455:462	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	5	29	theme	transcriptional	1146:1160	arg1	signatures					1162:1171	the transcriptional signatures	1142:1171	the transcriptional signatures of cell division	1142:1188	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	7	30	theme	molecular	1785:1793	arg1	force					1795:1799	a polyfunctional molecular force	1768:1799	a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis	1768:1903	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	7	31	dep	magnitude	1814:1822	arg1	the					1810:1812	the	1810:1812	the	1810:1812	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	2	32	theme	compromised	376:386	arg1	effects					388:394	compromised effects	376:394	compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD)	376:453	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	0	33	theme	Dietary	0:6	arg1	acid					20:23	Dietary palmitoleic acid	0:23	Dietary palmitoleic acid	0:23	Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis.
37203220	5	34	theme	muciniphila	1230:1240	arg1	signatures					1162:1171	the transcriptional signatures	1142:1171	the transcriptional signatures of cell division	1142:1188	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	5	34	theme	muciniphila	1230:1240	arg1	process					1207:1213	biosynthetic process	1194:1213	biosynthetic process of Akkermansia muciniphila	1194:1240	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	2	35	theme	inflammatory	421:432	arg1	IBD					450:452	IBD	450:452	IBD	450:452	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	35	theme	inflammatory	421:432	arg1	diseases					440:447	inflammatory bowel diseases	421:447	inflammatory bowel diseases (IBD)	421:453	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	1	36	theme	gut	295:297	arg1	microbiota					299:308	gut microbiota	295:308	gut microbiota	295:308	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	7	37	theme	POA	1761:1763	arg1	importance					1747:1756	the critical importance	1734:1756	the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis	1734:1903	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	5	38	theme	gut	1395:1397	arg1	microbiota					1399:1408	gut microbiota	1395:1408	gut microbiota	1395:1408	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	2	39	theme	therapy	410:416	arg1	effects					388:394	compromised effects	376:394	compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD)	376:453	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	39	theme	therapy	410:416	arg1	failures					364:371	failures	364:371	failures	364:371	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	0	40	theme	gut	36:38	arg1	microbiota					40:49	gut microbiota	36:49	gut microbiota	36:49	Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis.
37203220	5	41	theme	biosynthetic	1194:1205	arg1	process					1207:1213	biosynthetic process	1194:1213	biosynthetic process of Akkermansia muciniphila	1194:1240	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	6	42	theme	control	1459:1465	arg1	microbiota					1472:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	6	43	theme	gut	1468:1470	arg1	microbiota					1472:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	7	44	theme	therapeutic	1942:1952	arg1	strategy					1954:1961	a new potential therapeutic strategy	1926:1961	a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases	1926:2015	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	3	45	theme	Dietary	694:700	arg1	POA					702:704	Dietary POA	694:704	Dietary POA	694:704	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	7	46	theme	abenteric	1985:1993	arg1	diseases					2008:2015	intestinal or abenteric inflammatory diseases	1971:2015	intestinal or abenteric inflammatory diseases	1971:2015	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	7	47	theme	new	1928:1930	arg1	strategy					1954:1961	a new potential therapeutic strategy	1926:1961	a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases	1926:2015	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	5	48	theme	muciniphila	1305:1315	arg1	abundance					1280:1288	abundance	1280:1288	abundance	1280:1288	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	5	48	theme	muciniphila	1305:1315	arg1	growth					1269:1274	growth	1269:1274	growth	1269:1274	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	4	49	attach	derived	962:968	arg1	patients					996:1003	Crohn's disease (CD) patients	975:1003	Crohn's disease (CD) patients	975:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	4	49	attach	derived	962:968	arg2	tissues					954:960	cultured inflamed colon tissues	930:960	cultured inflamed colon tissues derived from Crohn's disease (CD) patients	930:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	4	50	theme	tissue	1076:1081	arg1	repairment					1083:1092	appreciable tissue repairment	1064:1092	appreciable tissue repairment	1064:1092	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	3	51	theme	mucosal	719:725	arg1	barriers					727:734	gut mucosal barriers	715:734	gut mucosal barriers	715:734	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	6	52	theme	recipient	1551:1559	arg1	mice					1561:1564	anti-TNF-α mAb-treated recipient mice	1528:1564	anti-TNF-α mAb-treated recipient mice	1528:1564	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	2	53	theme	lipid	623:627	arg1	metabolism					629:638	aberrant lipid metabolism	614:638	aberrant lipid metabolism	614:638	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	6	54	theme	anti-TNF-α	1528:1537	arg1	mice					1561:1564	anti-TNF-α mAb-treated recipient mice	1528:1564	anti-TNF-α mAb-treated recipient mice	1528:1564	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	3	55	theme	inflammatory	745:756	arg1	infiltrations					763:775	inflammatory cell infiltrations	745:775	inflammatory cell infiltrations	745:775	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	6	56	theme	synergistic	1644:1654	arg1	protections					1656:1666	significant synergistic protections	1632:1666	significant synergistic protections against colitis in mice	1632:1690	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	2	57	theme	lower	654:658	arg1	levels					660:665	lower levels	654:665	lower levels of palmitoleic acid (POA)	654:691	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	1	58	theme	human	188:192	arg1	health					194:199	human health	188:199	human health	188:199	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	2	59	from	failures	364:371	arg1	IBD					450:452	IBD	450:452	IBD	450:452	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	59	from	failures	364:371	arg1	diseases					440:447	inflammatory bowel diseases	421:447	inflammatory bowel diseases (IBD)	421:453	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	6	60	theme	Akkermansia	1601:1611	arg1	muciniphila					1613:1623	Akkermansia muciniphila	1601:1623	Akkermansia muciniphila	1601:1623	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	6	61	theme	POA	1592:1594	arg1	co-administration					1571:1587	co-administration	1571:1587	co-administration of POA with Akkermansia muciniphila	1571:1623	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	2	62	theme	extensive	548:556	arg1	inflammation					569:580	extensive unresolved inflammation	548:580	extensive unresolved inflammation	548:580	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	3	63	theme	IL-6	806:809	arg1	expressions					781:791	expressions	781:791	expressions of TNF-α and IL-6	781:809	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	3	63	theme	IL-6	806:809	arg1	infiltrations					763:775	inflammatory cell infiltrations	745:775	inflammatory cell infiltrations	745:775	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	4	64	theme	Ex	900:901	arg1	treatment					908:916	Ex vivo treatment	900:916	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients	900:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	3	65	theme	TNF-α	796:800	arg1	expressions					781:791	expressions	781:791	expressions of TNF-α and IL-6	781:809	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	3	65	theme	TNF-α	796:800	arg1	infiltrations					763:775	inflammatory cell infiltrations	745:775	inflammatory cell infiltrations	745:775	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	4	66	from	treatment	908:916	arg1	tissues					954:960	cultured inflamed colon tissues	930:960	cultured inflamed colon tissues derived from Crohn's disease (CD) patients	930:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	1	67	theme	microbiota	131:140	arg1	Magnitude					100:108	Magnitude	100:108	Magnitude	100:108	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	1	67	theme	microbiota	131:140	arg1	diversity					114:122	diversity	114:122	diversity	114:122	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	3	68	theme	chronic	874:880	arg1	models					892:897	both acute and chronic IBD mouse models	859:897	both acute and chronic IBD mouse models	859:897	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	2	69	theme	failed	583:588	arg1	repairment					598:607	failed mucosal repairment	583:607	failed mucosal repairment	583:607	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	1	70	theme	metabolic	146:154	arg1	systems					156:162	metabolic systems	146:162	metabolic systems	146:162	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	3	71	theme	acute	864:868	arg1	models					892:897	both acute and chronic IBD mouse models	859:897	both acute and chronic IBD mouse models	859:897	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	5	72	theme	gut	1320:1322	arg1	microbiota					1324:1333	gut microbiota	1320:1333	gut microbiota	1320:1333	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	5	73	theme	cell	1176:1179	arg1	division					1181:1188	cell division	1176:1188	cell division	1176:1188	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	1	74	from	shaping	180:186	arg1	critical					168:175	critical	168:175	critical	168:175	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	4	75	theme	disease	983:989	arg1	patients					996:1003	Crohn's disease (CD) patients	975:1003	Crohn's disease (CD) patients	975:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	1	76	from	critical	168:175	arg1	shaping					180:186	shaping human health and diseases	180:212	shaping human health and diseases	180:212	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	3	77	theme	mouse	886:890	arg1	models					892:897	both acute and chronic IBD mouse models	859:897	both acute and chronic IBD mouse models	859:897	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	4	78	theme	colon	948:952	arg1	tissues					954:960	cultured inflamed colon tissues	930:960	cultured inflamed colon tissues derived from Crohn's disease (CD) patients	930:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	2	79	theme	palmitoleic	670:680	arg1	acid					682:685	palmitoleic acid	670:685	palmitoleic acid (POA)	670:691	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	79	theme	palmitoleic	670:680	arg1	POA					688:690	POA	688:690	POA	688:690	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	5	80	theme	microbiota	1399:1408	arg1	structures					1381:1390	structures	1381:1390	structures	1381:1390	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	5	80	theme	microbiota	1399:1408	arg1	composition					1365:1375	composition	1365:1375	composition	1365:1375	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	3	81	theme	anti-TNF-α	837:846	arg1	therapy					848:854	anti-TNF-α therapy	837:854	anti-TNF-α therapy	837:854	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	6	82	theme	such	1428:1431	arg1	microbiota					1472:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	such POA-reprogrammed, but not control, gut microbiota	1428:1481	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	2	83	with	dysbacteriosis	496:509	arg1	inflammation					569:580	extensive unresolved inflammation	548:580	extensive unresolved inflammation	548:580	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	83	with	dysbacteriosis	496:509	arg1	bacteria					538:545	more pro-inflammatory bacteria	516:545	more pro-inflammatory bacteria	516:545	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	83	with	dysbacteriosis	496:509	arg1	metabolism					629:638	aberrant lipid metabolism	614:638	aberrant lipid metabolism	614:638	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	83	with	dysbacteriosis	496:509	arg1	repairment					598:607	failed mucosal repairment	583:607	failed mucosal repairment	583:607	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	83	with	dysbacteriosis	496:509	arg1	levels					660:665	lower levels	654:665	lower levels of palmitoleic acid (POA)	654:691	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	6	84	from	protections	1656:1666	arg1	mice					1687:1690	mice	1687:1690	mice	1687:1690	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	0	85	theme	palmitoleic	8:18	arg1	acid					20:23	Dietary palmitoleic acid	0:23	Dietary palmitoleic acid	0:23	Dietary palmitoleic acid reprograms gut microbiota and improves biological therapy against colitis.
37203220	4	86	theme	pro-inflammatory	1013:1028	arg1	signaling/cytokines					1030:1048	pro-inflammatory signaling/cytokines	1013:1048	pro-inflammatory signaling/cytokines	1013:1048	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	7	87	theme	gut	1841:1843	arg1	microbiota					1845:1854	gut microbiota	1841:1854	gut microbiota	1841:1854	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	5	88	from	abundance	1280:1288	arg1	microbiota					1324:1333	gut microbiota	1320:1333	gut microbiota	1320:1333	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	4	89	with	treatment	908:916	arg1	POA					923:925	POA	923:925	POA	923:925	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	2	90	theme	bowel	434:438	arg1	IBD					450:452	IBD	450:452	IBD	450:452	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	90	theme	bowel	434:438	arg1	diseases					440:447	inflammatory bowel diseases	421:447	inflammatory bowel diseases (IBD)	421:453	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	6	91	theme	better	1491:1496	arg1	protection					1498:1507	better protection	1491:1507	better protection against colitis	1491:1523	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	2	92	theme	anti-TNF-α	399:408	arg1	therapy					410:416	anti-TNF-α therapy	399:416	anti-TNF-α therapy	399:416	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	6	93	theme	microbiota	1472:1481	arg1	transfer					1416:1423	Oral transfer	1411:1423	Oral transfer of such POA-reprogrammed, but not control, gut microbiota	1411:1481	Oral transfer of such POA-reprogrammed, but not control, gut microbiota induced better protection against colitis in anti-TNF-α mAb-treated recipient mice, and co-administration of POA with Akkermansia muciniphila showed significant synergistic protections against colitis in mice.
37203220	7	94	theme	inflammatory	1995:2006	arg1	diseases					2008:2015	intestinal or abenteric inflammatory diseases	1971:2015	intestinal or abenteric inflammatory diseases	1971:2015	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	7	95	theme	intestinal	1971:1980	arg1	diseases					2008:2015	intestinal or abenteric inflammatory diseases	1971:2015	intestinal or abenteric inflammatory diseases	1971:2015	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	4	96	theme	cultured	930:937	arg1	tissues					954:960	cultured inflamed colon tissues	930:960	cultured inflamed colon tissues derived from Crohn's disease (CD) patients	930:1003	Ex vivo treatment with POA in cultured inflamed colon tissues derived from Crohn's disease (CD) patients reduced pro-inflammatory signaling/cytokines and conferred appreciable tissue repairment.
37203220	1	97	dep	shaping	180:186	arg1	diseases					205:212	diseases	205:212	diseases	205:212	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	1	97	dep	shaping	180:186	arg1	health					194:199	human health	188:199	human health	188:199	Magnitude and diversity of gut microbiota and metabolic systems are critical in shaping human health and diseases, but it remains largely unclear how complex metabolites may selectively regulate gut microbiota and determine health and diseases.
37203220	5	98	theme	Akkermansia	1218:1228	arg1	muciniphila					1230:1240	Akkermansia muciniphila	1218:1240	Akkermansia muciniphila	1218:1240	Mechanistically, POA significantly upregulated the transcriptional signatures of cell division and biosynthetic process of Akkermansia muciniphila, selectively increased the growth and abundance of Akkermansia muciniphila in gut microbiota, and further reprogrammed the composition and structures of gut microbiota.
37203220	2	99	from	effects	388:394	arg1	IBD					450:452	IBD	450:452	IBD	450:452	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	2	99	from	effects	388:394	arg1	diseases					440:447	inflammatory bowel diseases	421:447	inflammatory bowel diseases (IBD)	421:453	Here, we show that failures or compromised effects of anti-TNF-α therapy in inflammatory bowel diseases (IBD) patients were correlated with intestinal dysbacteriosis with more pro-inflammatory bacteria, extensive unresolved inflammation, failed mucosal repairment, and aberrant lipid metabolism, particularly lower levels of palmitoleic acid (POA).
37203220	3	100	from	efficacy	825:832	arg1	models					892:897	both acute and chronic IBD mouse models	859:897	both acute and chronic IBD mouse models	859:897	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
37203220	7	101	theme	potential	1932:1940	arg1	strategy					1954:1961	a new potential therapeutic strategy	1926:1961	a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases	1926:2015	Collectively, this work not only reveals the critical importance of POA as a polyfunctional molecular force to shape the magnitude and diversity of gut microbiota and therefore promote the intestinal homeostasis, but also implicates a new potential therapeutic strategy against intestinal or abenteric inflammatory diseases.
37203220	3	102	theme	gut	715:717	arg1	barriers					727:734	gut mucosal barriers	715:734	gut mucosal barriers	715:734	Dietary POA repaired gut mucosal barriers, reduced inflammatory cell infiltrations and expressions of TNF-α and IL-6, and improved efficacy of anti-TNF-α therapy in both acute and chronic IBD mouse models.
36213783	4	0	from	arteries	608:615	arg1	kidney					620:625	kidney	620:625	kidney of klotho mutant (kl/kl) mice	620:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	7	1	theme	CYP27B1	983:989	arg1	protein					991:997	the accumulated CYP27B1 protein	967:997	the accumulated CYP27B1 protein	967:997	We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice.
36213783	2	2	theme	aortic	387:392	arg1	media					394:398	aortic media	387:398	aortic media	387:398	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	8	3	from	mutation	1119:1126	arg1	CYP27B1					1226:1232	CYP27B1	1226:1232	CYP27B1	1226:1232	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	5	4	theme	high	742:745	arg1	condition					757:765	high phosphate condition	742:765	high phosphate condition	742:765	Under high phosphate condition, overexpression of CYP27B1 induced calcification and osteocalcin mRNA expression in the VSMCs.
36213783	6	5	theme	phosphate-induced	902:918	arg1	calcification					920:932	high phosphate-induced calcification	897:932	high phosphate-induced calcification of the VSMCs	897:945	Inversely, siRNA-CYP27B1 inhibited high phosphate-induced calcification of the VSMCs.
36213783	1	6	from	rate	181:184	arg1	patients					218:225	chronic kidney disease (CKD) patients	189:225	chronic kidney disease (CKD) patients	189:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	8	7	theme	phosphate	1304:1312	arg1	medium					1314:1319	high phosphate medium	1299:1319	high phosphate medium	1299:1319	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	4	8	theme	calcified	577:585	arg1	lesions					587:593	the calcified lesions	573:593	the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice	573:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	4	9	from	aorta	598:602	arg1	kidney					620:625	kidney	620:625	kidney of klotho mutant (kl/kl) mice	620:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	4	10	dep	found	526:530	arg1	co-localization					541:555	the co-localization	537:555	the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice	537:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	2	11	theme	smooth	324:329	arg1	cells					338:342	vascular smooth muscle cells	315:342	vascular smooth muscle cells (VSMCs)	315:350	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	2	11	theme	smooth	324:329	arg1	VSMCs					345:349	VSMCs	345:349	VSMCs	345:349	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	2	12	theme	muscle	331:336	arg1	cells					338:342	vascular smooth muscle cells	315:342	vascular smooth muscle cells (VSMCs)	315:350	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	2	12	theme	muscle	331:336	arg1	VSMCs					345:349	VSMCs	345:349	VSMCs	345:349	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	4	13	theme	aorta	598:602	arg1	lesions					587:593	the calcified lesions	573:593	the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice	573:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	1	14	theme	Vascular	76:83	arg1	calcification					85:97	Vascular calcification	76:97	Vascular calcification	76:97	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	1	14	theme	Vascular	76:83	arg1	pathogenesis					115:126	an important pathogenesis	102:126	an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients	102:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	2	15	from	calcification	370:382	arg1	media					394:398	aortic media	387:398	aortic media	387:398	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	2	16	theme	vascular	315:322	arg1	cells					338:342	vascular smooth muscle cells	315:342	vascular smooth muscle cells (VSMCs)	315:350	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	2	16	theme	vascular	315:322	arg1	VSMCs					345:349	VSMCs	345:349	VSMCs	345:349	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	9	17	theme	ectopic	1459:1465	arg1	calcification					1467:1479	ectopic calcification	1459:1479	ectopic calcification of VSMCs under hyperphosphatemia	1459:1512	These results suggest that extra-renal expression of glycosylated CYP27B1 would be required for ectopic calcification of VSMCs under hyperphosphatemia.
36213783	8	18	theme	calcium	1245:1251	arg1	deposition					1253:1262	calcium deposition	1245:1262	calcium deposition	1245:1262	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	4	19	from	lesions	587:593	arg1	kidney					620:625	kidney	620:625	kidney of klotho mutant (kl/kl) mice	620:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	8	20	from	expression	1268:1277	arg1	VSMCs					1324:1328	VSMCs	1324:1328	VSMCs	1324:1328	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	21	from	deposition	1253:1262	arg1	VSMCs					1324:1328	VSMCs	1324:1328	VSMCs	1324:1328	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	1	22	theme	chronic	189:195	arg1	disease					204:210	chronic kidney disease	189:210	chronic kidney disease (CKD) patients	189:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	1	22	theme	chronic	189:195	arg1	CKD					213:215	CKD	213:215	CKD	213:215	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	9	23	theme	CYP27B1	1429:1435	arg1	expression					1402:1411	extra-renal expression	1390:1411	extra-renal expression of glycosylated CYP27B1	1390:1435	These results suggest that extra-renal expression of glycosylated CYP27B1 would be required for ectopic calcification of VSMCs under hyperphosphatemia.
36213783	1	24	from	disease	154:160	arg1	patients					218:225	chronic kidney disease (CKD) patients	189:225	chronic kidney disease (CKD) patients	189:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	5	25	theme	CYP27B1	786:792	arg1	overexpression					768:781	overexpression	768:781	overexpression of CYP27B1	768:792	Under high phosphate condition, overexpression of CYP27B1 induced calcification and osteocalcin mRNA expression in the VSMCs.
36213783	1	26	theme	kidney	197:202	arg1	disease					204:210	chronic kidney disease	189:210	chronic kidney disease (CKD) patients	189:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	1	26	theme	kidney	197:202	arg1	CKD					213:215	CKD	213:215	CKD	213:215	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	8	27	theme	glycosylation	1141:1153	arg1	N310A					1161:1165	N310A	1161:1165	N310A	1161:1165	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	27	theme	glycosylation	1141:1153	arg1	site					1155:1158	N-linked glycosylation site	1132:1158	N-linked glycosylation site (N310A)	1132:1166	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	28	theme	N-linked	1132:1139	arg1	N310A					1161:1165	N310A	1161:1165	N310A	1161:1165	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	28	theme	N-linked	1132:1139	arg1	site					1155:1158	N-linked glycosylation site	1132:1158	N-linked glycosylation site (N310A)	1132:1166	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	4	29	with	co-localization	541:555	arg1	lesions					587:593	the calcified lesions	573:593	the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice	573:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	9	30	gly	glycosylated	1416:1427	arg1	CYP27B1					1429:1435	glycosylated CYP27B1	1416:1435	glycosylated CYP27B1	1416:1435	These results suggest that extra-renal expression of glycosylated CYP27B1 would be required for ectopic calcification of VSMCs under hyperphosphatemia.
36213783	1	31	theme	disease	204:210	arg1	patients					218:225	chronic kidney disease (CKD) patients	189:225	chronic kidney disease (CKD) patients	189:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	0	32	theme	25-hydroxyvitamin	0:16	arg1	CYP27B1					36:42	CYP27B1	36:42	CYP27B1	36:42	25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification.
36213783	0	32	theme	25-hydroxyvitamin	0:16	arg1	D-1α-hydroxylase					18:33	25-hydroxyvitamin D-1α-hydroxylase	0:33	25-hydroxyvitamin D-1α-hydroxylase (CYP27B1)	0:43	25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification.
36213783	4	33	theme	CYP27B1	560:566	arg1	co-localization					541:555	the co-localization	537:555	the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice	537:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	1	34	theme	important	105:113	arg1	calcification					85:97	Vascular calcification	76:97	Vascular calcification	76:97	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	1	34	theme	important	105:113	arg1	pathogenesis					115:126	an important pathogenesis	102:126	an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients	102:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	2	35	theme	cardiac	401:407	arg1	valve					409:413	cardiac valve	401:413	cardiac valve	401:413	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	8	36	theme	O-linked	1187:1194	arg1	site					1210:1213	O-linked glycosylation site	1187:1213	O-linked glycosylation site (T439A)	1187:1221	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	36	theme	O-linked	1187:1194	arg1	T439A					1216:1220	T439A	1216:1220	T439A	1216:1220	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	9	37	theme	VSMCs	1484:1488	arg1	calcification					1467:1479	ectopic calcification	1459:1479	ectopic calcification of VSMCs under hyperphosphatemia	1459:1512	These results suggest that extra-renal expression of glycosylated CYP27B1 would be required for ectopic calcification of VSMCs under hyperphosphatemia.
36213783	8	38	theme	RUNX2	1282:1286	arg1	expression					1268:1277	expression	1268:1277	expression	1268:1277	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	38	theme	RUNX2	1282:1286	arg1	deposition					1253:1262	calcium deposition	1245:1262	calcium deposition	1245:1262	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	39	gly	glycosylation	1196:1208	arg2	T439A					1216:1220	T439A	1216:1220	T439A	1216:1220	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	39	gly	glycosylation	1196:1208	arg2	site					1210:1213	O-linked glycosylation site	1187:1213	O-linked glycosylation site (T439A)	1187:1221	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	2	40	theme	cells	338:342	arg1	transition					301:310	osteochondrogenic transition	283:310	osteochondrogenic transition of vascular smooth muscle cells (VSMCs)	283:350	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	8	41	link	O-linked	1187:1194	arg1	site					1210:1213	O-linked glycosylation site	1187:1213	O-linked glycosylation site (T439A)	1187:1221	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	41	link	O-linked	1187:1194	arg1	T439A					1216:1220	T439A	1216:1220	T439A	1216:1220	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	3	42	theme	calcification	475:487	arg1	mechanism					450:458	the detailed mechanism	437:458	the detailed mechanism of the ectopic calcification	437:487	However, the detailed mechanism of the ectopic calcification has been not clarified yet.
36213783	4	43	from	role	684:687	arg1	mineralization					707:720	the mineralization	703:720	the mineralization of the VSMCs	703:733	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	8	44	link	N-linked	1132:1139	arg1	N310A					1161:1165	N310A	1161:1165	N310A	1161:1165	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	44	link	N-linked	1132:1139	arg1	site					1155:1158	N-linked glycosylation site	1132:1158	N-linked glycosylation site (N310A)	1132:1166	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	1	45	theme	related	128:134	arg1	calcification					85:97	Vascular calcification	76:97	Vascular calcification	76:97	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	1	45	theme	related	128:134	arg1	pathogenesis					115:126	an important pathogenesis	102:126	an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients	102:225	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	5	46	theme	osteocalcin	820:830	arg1	expression					837:846	osteocalcin mRNA expression	820:846	osteocalcin mRNA expression	820:846	Under high phosphate condition, overexpression of CYP27B1 induced calcification and osteocalcin mRNA expression in the VSMCs.
36213783	5	47	theme	phosphate	747:755	arg1	condition					757:765	high phosphate condition	742:765	high phosphate condition	742:765	Under high phosphate condition, overexpression of CYP27B1 induced calcification and osteocalcin mRNA expression in the VSMCs.
36213783	2	48	theme	ectopic	362:368	arg1	calcification					370:382	ectopic calcification	362:382	ectopic calcification in aortic media	362:398	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	8	49	theme	CYP27B1-N310A	1074:1086	arg1	overexpression					1056:1069	overexpression	1056:1069	overexpression	1056:1069	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	49	theme	CYP27B1-N310A	1074:1086	arg1	mutation					1119:1126	a mutation	1117:1126	a mutation for N-linked glycosylation site (N310A)	1117:1166	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	3	50	theme	detailed	441:448	arg1	mechanism					450:458	the detailed mechanism	437:458	the detailed mechanism of the ectopic calcification	437:487	However, the detailed mechanism of the ectopic calcification has been not clarified yet.
36213783	5	51	theme	mRNA	832:835	arg1	expression					837:846	osteocalcin mRNA expression	820:846	osteocalcin mRNA expression	820:846	Under high phosphate condition, overexpression of CYP27B1 induced calcification and osteocalcin mRNA expression in the VSMCs.
36213783	8	52	theme	glycosylation	1196:1208	arg1	site					1210:1213	O-linked glycosylation site	1187:1213	O-linked glycosylation site (T439A)	1187:1221	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	52	theme	glycosylation	1196:1208	arg1	T439A					1216:1220	T439A	1216:1220	T439A	1216:1220	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	53	theme	CYP27B1-T439A	1092:1104	arg1	overexpression					1056:1069	overexpression	1056:1069	overexpression	1056:1069	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	53	theme	CYP27B1-T439A	1092:1104	arg1	mutation					1119:1126	a mutation	1117:1126	a mutation for N-linked glycosylation site (N310A)	1117:1166	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	4	54	theme	VSMCs	729:733	arg1	mineralization					707:720	the mineralization	703:720	the mineralization of the VSMCs	703:733	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	2	55	from	valve	409:413	arg1	media					394:398	aortic media	387:398	aortic media	387:398	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	3	56	theme	ectopic	467:473	arg1	calcification					475:487	the ectopic calcification	463:487	the ectopic calcification	463:487	However, the detailed mechanism of the ectopic calcification has been not clarified yet.
36213783	7	57	theme	mice	1039:1042	arg1	kidney					1023:1028	the kidney	1019:1028	the kidney of kl/kl mice	1019:1042	We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice.
36213783	8	58	theme	wild-type	1344:1352	arg1	CYP27B1					1354:1360	wild-type CYP27B1	1344:1360	wild-type CYP27B1	1344:1360	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	0	59	theme	ectopic	53:59	arg1	calcification					61:73	ectopic calcification	53:73	ectopic calcification	53:73	25-hydroxyvitamin D-1α-hydroxylase (CYP27B1) induces ectopic calcification.
36213783	6	60	theme	VSMCs	941:945	arg1	calcification					920:932	high phosphate-induced calcification	897:932	high phosphate-induced calcification of the VSMCs	897:945	Inversely, siRNA-CYP27B1 inhibited high phosphate-induced calcification of the VSMCs.
36213783	8	61	from	mutation	1174:1181	arg1	CYP27B1					1226:1232	CYP27B1	1226:1232	CYP27B1	1226:1232	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	62	gly	glycosylation	1141:1153	arg2	N310A					1161:1165	N310A	1161:1165	N310A	1161:1165	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	8	62	gly	glycosylation	1141:1153	arg2	site					1155:1158	N-linked glycosylation site	1132:1158	N-linked glycosylation site (N310A)	1132:1166	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	1	63	theme	cardiovascular	139:152	arg1	disease					154:160	cardiovascular disease	139:160	cardiovascular disease	139:160	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	7	64	gly	glycosylated	1003:1014	arg1	protein					991:997	the accumulated CYP27B1 protein	967:997	the accumulated CYP27B1 protein	967:997	We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice.
36213783	7	64	gly	glycosylated	1003:1014	arg2	protein					991:997	the accumulated CYP27B1 protein	967:997	the accumulated CYP27B1 protein	967:997	We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice.
36213783	7	64	gly	glycosylated	1003:1014	arg1	kidney					1023:1028	the kidney	1019:1028	the kidney of kl/kl mice	1019:1042	We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice.
36213783	4	65	theme	mice	652:655	arg1	kidney					620:625	kidney	620:625	kidney of klotho mutant (kl/kl) mice	620:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	2	66	theme	osteochondrogenic	283:299	arg1	transition					301:310	osteochondrogenic transition	283:310	osteochondrogenic transition of vascular smooth muscle cells (VSMCs)	283:350	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	4	67	theme	arteries	608:615	arg1	lesions					587:593	the calcified lesions	573:593	the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice	573:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	7	68	theme	kl/kl	1033:1037	arg1	mice					1039:1042	kl/kl mice	1033:1042	kl/kl mice	1033:1042	We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice.
36213783	8	69	theme	high	1299:1302	arg1	medium					1314:1319	high phosphate medium	1299:1319	high phosphate medium	1299:1319	Therefore, overexpression of CYP27B1-N310A and CYP27B1-T439A, which are a mutation for N-linked glycosylation site (N310A) and a mutation for O-linked glycosylation site (T439A) in CYP27B1, decreased calcium deposition and expression of RUNX2 induced by high phosphate medium in VSMCs compared with wild-type CYP27B1.
36213783	4	70	theme	CYP27B1	692:698	arg1	role					684:687	the role	680:687	the role of CYP27B1 in the mineralization of the VSMCs	680:733	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	4	71	from	kidney	620:625	arg1	lesions					587:593	the calcified lesions	573:593	the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice	573:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	4	72	theme	mutant	637:642	arg1	mice					652:655	klotho mutant (kl/kl) mice	630:655	klotho mutant (kl/kl) mice	630:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	2	73	from	kidney	420:425	arg1	media					394:398	aortic media	387:398	aortic media	387:398	It has been well-known that hyper-phosphatemia induces osteochondrogenic transition of vascular smooth muscle cells (VSMCs) resulting ectopic calcification in aortic media, cardiac valve, and kidney.
36213783	1	74	theme	high	166:169	arg1	rate					181:184	high mortality rate	166:184	high mortality rate	166:184	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	4	75	theme	kl/kl	645:649	arg1	mice					652:655	klotho mutant (kl/kl) mice	630:655	klotho mutant (kl/kl) mice	630:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	6	76	theme	high	897:900	arg1	calcification					920:932	high phosphate-induced calcification	897:932	high phosphate-induced calcification of the VSMCs	897:945	Inversely, siRNA-CYP27B1 inhibited high phosphate-induced calcification of the VSMCs.
36213783	9	77	theme	glycosylated	1416:1427	arg1	CYP27B1					1429:1435	glycosylated CYP27B1	1416:1435	glycosylated CYP27B1	1416:1435	These results suggest that extra-renal expression of glycosylated CYP27B1 would be required for ectopic calcification of VSMCs under hyperphosphatemia.
36213783	4	78	theme	klotho	630:635	arg1	mice					652:655	klotho mutant (kl/kl) mice	630:655	klotho mutant (kl/kl) mice	630:655	Here, we found that the co-localization of CYP27B1 with the calcified lesions of aorta and arteries in kidney of klotho mutant (kl/kl) mice, and then investigated the role of CYP27B1 in the mineralization of the VSMCs.
36213783	7	79	theme	accumulated	971:981	arg1	protein					991:997	the accumulated CYP27B1 protein	967:997	the accumulated CYP27B1 protein	967:997	We also found that the accumulated CYP27B1 protein was glycosylated in the kidney of kl/kl mice.
36213783	1	80	theme	mortality	171:179	arg1	rate					181:184	high mortality rate	166:184	high mortality rate	166:184	Vascular calcification is an important pathogenesis related to cardiovascular disease and high mortality rate in chronic kidney disease (CKD) patients.
36213783	9	81	theme	extra-renal	1390:1400	arg1	expression					1402:1411	extra-renal expression	1390:1411	extra-renal expression of glycosylated CYP27B1	1390:1435	These results suggest that extra-renal expression of glycosylated CYP27B1 would be required for ectopic calcification of VSMCs under hyperphosphatemia.
37059251	6	0	theme	antinociceptive	843:857	arg1	activities					859:868	anti-inflammatory and antinociceptive activities	821:868	anti-inflammatory and antinociceptive activities	821:868	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	17	1	from	spent	2473:2477	arg1	apparatus					2495:2503	the rota-rod apparatus	2482:2503	the rota-rod apparatus	2482:2503	Also, majority fraction F33 did not affect the time mice spent in the rota-rod apparatus.
37059251	6	2	theme	ethnopharmacological	716:735	arg1	use					737:739	the ethnopharmacological use	712:739	the ethnopharmacological use of Lippia lacunosa	712:758	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	12	3	theme	edema	1800:1804	arg1	models					1786:1791	experimental models	1773:1791	experimental models of paw edema and mechanical allodynia induced by carrageenan	1773:1852	In experimental models of paw edema and mechanical allodynia induced by carrageenan (600 μg, 30 μL, i.pl.)
37059251	6	4	theme	anti-inflammatory	821:837	arg1	activities					859:868	anti-inflammatory and antinociceptive activities	821:868	anti-inflammatory and antinociceptive activities	821:868	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	1	5	dep	Mart	175:178	arg1	RELEVANCE					149:157	ETHNOPHARMACOLOGICAL RELEVANCE	128:157	ETHNOPHARMACOLOGICAL RELEVANCE	128:157	ETHNOPHARMACOLOGICAL RELEVANCE Lippia lacunosa Mart.
37059251	18	6	from	knowledge	2749:2757	arg1	use					2795:2797	the ancient ethnopharmacological use	2762:2797	the ancient ethnopharmacological use	2762:2797	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	7	theme	acute	2653:2657	arg1	inflammation					2659:2670	acute inflammation	2653:2670	acute inflammation	2653:2670	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	12	8	theme	mechanical	1810:1819	arg1	allodynia					1821:1829	mechanical allodynia	1810:1829	mechanical allodynia	1810:1829	In experimental models of paw edema and mechanical allodynia induced by carrageenan (600 μg, 30 μL, i.pl.)
37059251	6	9	theme	composition	805:815	arg1	evaluation					772:781	the evaluation	768:781	the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice	768:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	15	10	theme	mechanical	2248:2257	arg1	allodynia					2259:2267	mechanical allodynia	2248:2267	mechanical allodynia	2248:2267	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	6	11	dep	aimed	670:674	arg1	AIM					642:644	AIM	642:644	AIM OF THE STUDY	642:657	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	12	from	composition	805:815	arg1	mice					940:943	mice	940:943	mice	940:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	13	theme	scientific	688:697	arg1	knowledge					699:707	scientific knowledge	688:707	scientific knowledge on the ethnopharmacological use of Lippia lacunosa	688:758	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	2	14	theme	Atlantic	269:276	arg1	plateau					278:284	the Atlantic plateau	265:284	the Atlantic plateau	265:284	& Schauer is an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil.
37059251	9	15	theme	plate	1360:1364	arg1	tests					1366:1370	hot plate tests	1356:1370	hot plate tests	1356:1370	Mechanical allodynia induced by carrageenan and hot plate tests were employed to evaluate the antinociceptive activity.
37059251	12	16	theme	experimental	1773:1784	arg1	models					1786:1791	experimental models	1773:1791	experimental models of paw edema and mechanical allodynia induced by carrageenan	1773:1852	In experimental models of paw edema and mechanical allodynia induced by carrageenan (600 μg, 30 μL, i.pl.)
37059251	17	17	theme	majority	2422:2429	arg1	fraction					2431:2438	majority fraction F33	2422:2442	majority fraction F33	2422:2442	Also, majority fraction F33 did not affect the time mice spent in the rota-rod apparatus.
37059251	5	18	used	used	581:584	arg2	It					539:540	It	539:540	It	539:540	It is often confused with and, therefore, used interchangeably with L. rotundifolia and L. pseudothea.
37059251	7	19	theme	lacunosa	995:1002	arg1	extracts					1004:1011	L. lacunosa extracts	992:1011	L. lacunosa extracts	992:1011	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	13	20	theme	hexane	1900:1905	arg1	extract					1907:1913	hexane extract	1900:1913	hexane extract	1900:1913	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	6	21	theme	ethanolic	888:896	arg1	extracts					898:905	ethanolic extracts	888:905	ethanolic extracts	888:905	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	16	22	theme	essential	2323:2331	arg1	oil					2333:2335	essential oil	2323:2335	essential oil	2323:2335	The hexane extract, essential oil, and majority fraction F33 also reduced the heat-induced nociceptive response.
37059251	6	23	theme	hexane	877:882	arg1	composition					805:815	micromolecular composition	790:815	micromolecular composition	790:815	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	23	theme	hexane	877:882	arg1	activities					859:868	anti-inflammatory and antinociceptive activities	821:868	anti-inflammatory and antinociceptive activities	821:868	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	24	dep	hexane	877:882	arg1	the					873:875	the	873:875	the	873:875	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	7	25	theme	chemical	972:979	arg1	profile					981:987	The chemical profile	968:987	The chemical profile	968:987	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	18	26	theme	lacunosa	2618:2625	arg1	activity					2603:2610	the activity	2599:2610	the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2599:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	15	27	dep	extract	2138:2144	arg1	50					2147:2148	50	2147:2148	50	2147:2148	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	15	27	dep	extract	2138:2144	arg1	100 mg/kg					2153:2161	100 mg/kg	2153:2161	100 mg/kg	2153:2161	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	18	28	with	patients	2938:2945	arg1	inflammatory					2952:2963	inflammatory	2952:2963	inflammatory	2952:2963	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	28	with	patients	2938:2945	arg1	conditions					2977:2986	painful conditions	2969:2986	painful conditions	2969:2986	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	15	29	theme	essential	2168:2176	arg1	100 mg/kg					2183:2191	100 mg/kg	2183:2191	100 mg/kg	2183:2191	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	15	29	theme	essential	2168:2176	arg1	oil					2178:2180	essential oil	2168:2180	essential oil (100 mg/kg)	2168:2192	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	3	30	theme	chá	311:313	arg1	"					326:326	"chá de pedestre"	310:326	"chá de pedestre"	310:326	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	3	30	theme	chá	311:313	arg1	It					295:296	It	295:296	It	295:296	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	4	31	theme	characteristic	382:395	arg1	aroma					403:407	a characteristic mango aroma	380:407	a characteristic mango aroma	380:407	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	8	32	theme	Carrageenan-induced	1214:1232	arg1	edema					1238:1242	Carrageenan-induced paw edema	1214:1242	Carrageenan-induced paw edema	1214:1242	Carrageenan-induced paw edema was used to investigate the anti-inflammatory activity in mice.
37059251	0	33	theme	Lippia	75:80	arg1	lacunosa					82:89	Lippia lacunosa	75:89	Lippia lacunosa	75:89	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	13	34	theme	paw	2000:2002	arg1	edema					2004:2008	paw edema	2000:2008	paw edema	2000:2008	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	13	35	theme	extract	1907:1913	arg1	administration					1882:1895	the administration	1878:1895	the administration of hexane extract	1878:1913	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	10	36	theme	monoterpenes	1494:1505	arg1	%					1521:1521	13.81%	1516:1521	13.81%	1516:1521	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	10	36	theme	monoterpenes	1494:1505	arg1	RESULTS					1428:1434	RESULTS	1428:1434	RESULTS The main constituents found in the essential oil	1428:1483	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	10	36	theme	monoterpenes	1494:1505	arg1	myrcene					1507:1513	the monoterpenes myrcene	1490:1513	the monoterpenes myrcene (13.81%)	1490:1522	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	17	37	theme	rota-rod	2486:2493	arg1	apparatus					2495:2503	the rota-rod apparatus	2482:2503	the rota-rod apparatus	2482:2503	Also, majority fraction F33 did not affect the time mice spent in the rota-rod apparatus.
37059251	6	38	from	mice	940:943	arg1	composition					805:815	micromolecular composition	790:815	micromolecular composition	790:815	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	38	from	mice	940:943	arg1	activities					859:868	anti-inflammatory and antinociceptive activities	821:868	anti-inflammatory and antinociceptive activities	821:868	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	11	39	from	compounds	1729:1737	arg1	rich					1712:1715	rich	1712:1715	rich	1712:1715	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	13	40	dep	oil	1926:1928	arg1	or					1934:1935	50 or 100 mg/kg	1931:1945	50 or 100 mg/kg	1931:1945	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	13	40	dep	oil	1926:1928	arg1	p.o.					1948:1951	p.o.	1948:1951	p.o.	1948:1951	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	15	41	theme	hexane	2131:2136	arg1	extract					2138:2144	the hexane extract	2127:2144	the hexane extract (50 or 100 mg/kg)	2127:2162	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	15	42	theme	majority	2210:2217	arg1	fraction					2219:2226	the majority fraction	2206:2226	the majority fraction (10 mg/kg)	2206:2237	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	15	42	theme	majority	2210:2217	arg1	10 mg/kg					2229:2236	10 mg/kg	2229:2236	10 mg/kg	2229:2236	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	18	43	dep	CONCLUSIONS	2506:2516	arg1	CONCLUSIONS					2506:2516	CONCLUSIONS	2506:2516	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2506:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	43	dep	CONCLUSIONS	2506:2516	arg1	demonstration					2582:2594	the demonstration	2578:2594	the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2578:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	43	dep	CONCLUSIONS	2506:2516	arg1	elucidation					2522:2532	The elucidation	2518:2532	The elucidation of the composition of the essential oil	2518:2572	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	3	44	theme	pedestre	318:325	arg1	"					326:326	"chá de pedestre"	310:326	"chá de pedestre"	310:326	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	3	44	theme	pedestre	318:325	arg1	It					295:296	It	295:296	It	295:296	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	11	45	from	rich	1712:1715	arg1	mircenone					1759:1767	mircenone	1759:1767	mircenone	1759:1767	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	11	45	from	rich	1712:1715	arg1	F33					1740:1742	F33	1740:1742	F33	1740:1742	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	11	45	from	rich	1712:1715	arg1	ipsenone					1746:1753	ipsenone	1746:1753	ipsenone	1746:1753	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	11	45	from	rich	1712:1715	arg1	compounds					1729:1737	the main compounds	1720:1737	the main compounds (F33)	1720:1743	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	11	46	theme	main	1724:1727	arg1	mircenone					1759:1767	mircenone	1759:1767	mircenone	1759:1767	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	11	46	theme	main	1724:1727	arg1	F33					1740:1742	F33	1740:1742	F33	1740:1742	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	11	46	theme	main	1724:1727	arg1	ipsenone					1746:1753	ipsenone	1746:1753	ipsenone	1746:1753	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	11	46	theme	main	1724:1727	arg1	compounds					1729:1737	the main compounds	1720:1737	the main compounds (F33)	1720:1743	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	7	47	theme	Gas	1124:1126	arg1	Chromatography					1128:1141	Gas Chromatography	1124:1141	Gas Chromatography (GC)	1124:1146	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	47	theme	Gas	1124:1126	arg1	GC					1144:1145	GC	1144:1145	GC	1144:1145	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	18	48	theme	essential	2560:2568	arg1	oil					2570:2572	the essential oil	2556:2572	the essential oil	2556:2572	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	0	49	theme	anti-inflammatory	47:63	arg1	effect					65:70	antinociceptive and anti-inflammatory effect	27:70	antinociceptive and anti-inflammatory effect of Lippia lacunosa	27:89	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	0	49	theme	anti-inflammatory	47:63	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	18	50	theme	herbal	2879:2884	arg1	medicine					2886:2893	herbal medicine	2879:2893	herbal medicine	2879:2893	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	6	51	from	knowledge	699:707	arg1	use					737:739	the ethnopharmacological use	712:739	the ethnopharmacological use of Lippia lacunosa	712:758	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	52	from	fractions	927:935	arg1	mice					940:943	mice	940:943	mice	940:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	8	53	theme	anti-inflammatory	1272:1288	arg1	activity					1290:1297	the anti-inflammatory activity	1268:1297	the anti-inflammatory activity in mice	1268:1305	Carrageenan-induced paw edema was used to investigate the anti-inflammatory activity in mice.
37059251	7	54	dep	MATERIALS	946:954	arg1	profile					981:987	The chemical profile	968:987	The chemical profile	968:987	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	4	55	contain	has	376:378	arg2	aroma					403:407	a characteristic mango aroma	380:407	a characteristic mango aroma	380:407	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	4	55	contain	has	376:378	arg1	species					368:374	This species	363:374	This species	363:374	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	0	56	theme	Chemical	0:7	arg1	effect					65:70	antinociceptive and anti-inflammatory effect	27:70	antinociceptive and anti-inflammatory effect of Lippia lacunosa	27:89	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	0	56	theme	Chemical	0:7	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	0	56	theme	Chemical	0:7	arg1	species					94:100	a species	92:100	a species used by the Bandeirantes	92:125	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	18	57	theme	nociceptive	2694:2704	arg1	models					2684:2689	models	2684:2689	models of nociceptive and inflammatory pain	2684:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	3	58	theme	rosmaninho	333:342	arg1	It					295:296	It	295:296	It	295:296	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	3	58	theme	rosmaninho	333:342	arg1	"					343:343	"rosmaninho"	332:343	"rosmaninho"	332:343	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	6	59	from	extracts	898:905	arg1	mice					940:943	mice	940:943	mice	940:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	4	60	theme	relaxing	490:497	arg1	baths					499:503	relaxing baths	490:503	relaxing baths	490:503	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	7	61	theme	Liquid	1094:1099	arg1	UPLC					1117:1120	UPLC	1117:1120	UPLC	1117:1120	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	61	theme	Liquid	1094:1099	arg1	Chromatography					1101:1114	Ultra-Performance Liquid Chromatography	1076:1114	Ultra-Performance Liquid Chromatography (UPLC)	1076:1121	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	17	62	theme	time	2463:2466	arg1	spent					2473:2477	the time mice spent	2459:2477	the time mice spent in the rota-rod apparatus	2459:2503	Also, majority fraction F33 did not affect the time mice spent in the rota-rod apparatus.
37059251	17	63	theme	mice	2468:2471	arg1	spent					2473:2477	the time mice spent	2459:2477	the time mice spent in the rota-rod apparatus	2459:2503	Also, majority fraction F33 did not affect the time mice spent in the rota-rod apparatus.
37059251	7	64	theme	Column	1149:1154	arg1	CC					1172:1173	CC	1172:1173	CC	1172:1173	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	64	theme	Column	1149:1154	arg1	Chromatography					1156:1169	Column Chromatography	1149:1169	Column Chromatography (CC)	1149:1174	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	11	65	theme	essential	1679:1687	arg1	oil					1689:1691	essential oil	1679:1691	essential oil	1679:1691	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	18	66	theme	inflammatory	2710:2721	arg1	pain					2723:2726	inflammatory pain	2710:2726	inflammatory pain	2710:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	6	67	theme	lacunosa	751:758	arg1	use					737:739	the ethnopharmacological use	712:739	the ethnopharmacological use of Lippia lacunosa	712:758	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	7	68	theme	Layer	1186:1190	arg1	TLC					1208:1210	TLC	1208:1210	TLC	1208:1210	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	68	theme	Layer	1186:1190	arg1	Chromatography					1192:1205	Thin Layer Chromatography	1181:1205	Thin Layer Chromatography (TLC)	1181:1211	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	10	69	theme	main	1440:1443	arg1	constituents					1445:1456	The main constituents	1436:1456	RESULTS The main constituents found in the essential oil	1428:1483	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	15	70	theme	evaluation	2284:2293	arg1	period					2295:2300	the evaluation period	2280:2300	the evaluation period	2280:2300	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	16	71	theme	majority	2342:2349	arg1	F33					2360:2362	majority fraction F33	2342:2362	majority fraction F33	2342:2362	The hexane extract, essential oil, and majority fraction F33 also reduced the heat-induced nociceptive response.
37059251	18	72	from	demonstration	2582:2594	arg1	models					2643:2648	experimental models	2630:2648	experimental models of acute inflammation	2630:2670	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	72	from	demonstration	2582:2594	arg1	models					2684:2689	models	2684:2689	models of nociceptive and inflammatory pain	2684:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	2	73	theme	endemic	197:203	arg1	plant					205:209	an endemic plant	194:209	an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil	194:292	& Schauer is an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil.
37059251	2	73	theme	endemic	197:203	arg1	Schauer					183:189	Schauer	183:189	Schauer	183:189	& Schauer is an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil.
37059251	9	74	theme	Mechanical	1308:1317	arg1	allodynia					1319:1327	Mechanical allodynia	1308:1327	Mechanical allodynia induced by carrageenan and hot plate tests	1308:1370	Mechanical allodynia induced by carrageenan and hot plate tests were employed to evaluate the antinociceptive activity.
37059251	12	75	theme	allodynia	1821:1829	arg1	models					1786:1791	experimental models	1773:1791	experimental models of paw edema and mechanical allodynia induced by carrageenan	1773:1852	In experimental models of paw edema and mechanical allodynia induced by carrageenan (600 μg, 30 μL, i.pl.)
37059251	6	76	theme	activities	859:868	arg1	evaluation					772:781	the evaluation	768:781	the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice	768:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	77	dep	composition	805:815	arg1	the					786:788	the	786:788	the	786:788	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	78	from	activities	859:868	arg1	mice					940:943	mice	940:943	mice	940:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	7	79	theme	chromatographic	1044:1058	arg1	Chromatography					1128:1141	Gas Chromatography	1124:1141	Gas Chromatography (GC)	1124:1146	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	79	theme	chromatographic	1044:1058	arg1	Chromatography					1156:1169	Column Chromatography	1149:1169	Column Chromatography (CC)	1149:1174	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	79	theme	chromatographic	1044:1058	arg1	Chromatography					1192:1205	Thin Layer Chromatography	1181:1205	Thin Layer Chromatography (TLC)	1181:1211	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	79	theme	chromatographic	1044:1058	arg1	Chromatography					1101:1114	Ultra-Performance Liquid Chromatography	1076:1114	Ultra-Performance Liquid Chromatography (UPLC)	1076:1121	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	79	theme	chromatographic	1044:1058	arg1	methods					1060:1066	chromatographic methods	1044:1066	chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC)	1044:1211	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	8	80	from	activity	1290:1297	arg1	mice					1302:1305	mice	1302:1305	mice	1302:1305	Carrageenan-induced paw edema was used to investigate the anti-inflammatory activity in mice.
37059251	18	81	theme	composition	2541:2551	arg1	CONCLUSIONS					2506:2516	CONCLUSIONS	2506:2516	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2506:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	81	theme	composition	2541:2551	arg1	demonstration					2582:2594	the demonstration	2578:2594	the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2578:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	81	theme	composition	2541:2551	arg1	elucidation					2522:2532	The elucidation	2518:2532	The elucidation of the composition of the essential oil	2518:2572	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	82	from	models	2684:2689	arg1	CONCLUSIONS					2506:2516	CONCLUSIONS	2506:2516	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2506:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	82	from	models	2684:2689	arg1	demonstration					2582:2594	the demonstration	2578:2594	the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2578:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	82	from	models	2684:2689	arg1	elucidation					2522:2532	The elucidation	2518:2532	The elucidation of the composition of the essential oil	2518:2572	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	83	theme	inflammation	2659:2670	arg1	models					2643:2648	experimental models	2630:2648	experimental models of acute inflammation	2630:2670	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	12	84	theme	paw	1796:1798	arg1	edema					1800:1804	paw edema	1796:1804	paw edema	1796:1804	In experimental models of paw edema and mechanical allodynia induced by carrageenan (600 μg, 30 μL, i.pl.)
37059251	4	85	theme	foot	510:513	arg1	baths					515:519	foot baths	510:519	foot baths after long walks	510:536	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	7	86	theme	fractions	1017:1025	arg1	METHODS					960:966	METHODS	960:966	METHODS	960:966	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	86	theme	fractions	1017:1025	arg1	MATERIALS					946:954	MATERIALS	946:954	MATERIALS	946:954	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	10	87	theme	sesquiterpenes	1589:1602	arg1	elemol					1604:1609	sesquiterpenes elemol	1589:1609	sesquiterpenes elemol (7.30%)	1589:1617	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	10	87	theme	sesquiterpenes	1589:1602	arg1	%					1616:1616	7.30%	1612:1616	7.30%	1612:1616	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	9	88	theme	hot	1356:1358	arg1	tests					1366:1370	hot plate tests	1356:1370	hot plate tests	1356:1370	Mechanical allodynia induced by carrageenan and hot plate tests were employed to evaluate the antinociceptive activity.
37059251	6	89	theme	micromolecular	790:803	arg1	composition					805:815	micromolecular composition	790:815	micromolecular composition	790:815	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	7	90	theme	extracts	1004:1011	arg1	METHODS					960:966	METHODS	960:966	METHODS	960:966	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	90	theme	extracts	1004:1011	arg1	MATERIALS					946:954	MATERIALS	946:954	MATERIALS	946:954	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	14	91	theme	evaluation	2096:2105	arg1	2 nd h					2086:2091	the 2 nd h	2082:2091	the 2 nd h of evaluation	2082:2105	The ethanolic extract (100 mg/kg) reduced mechanical allodynia only in the 2 nd h of evaluation.
37059251	6	92	theme	essential	908:916	arg1	oil					918:920	essential oil	908:920	essential oil	908:920	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	7	93	theme	L.	992:993	arg1	extracts					1004:1011	L. lacunosa extracts	992:1011	L. lacunosa extracts	992:1011	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	6	94	theme	extracts	898:905	arg1	composition					805:815	micromolecular composition	790:815	micromolecular composition	790:815	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	6	94	theme	extracts	898:905	arg1	activities					859:868	anti-inflammatory and antinociceptive activities	821:868	anti-inflammatory and antinociceptive activities	821:868	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	2	95	dep	Serra	220:224	arg1	do					226:227	do	226:227	do	226:227	& Schauer is an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil.
37059251	3	96	from	"	326:326	arg1	medicine					353:360	folk medicine	348:360	folk medicine	348:360	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	18	97	from	elucidation	2522:2532	arg1	models					2643:2648	experimental models	2630:2648	experimental models of acute inflammation	2630:2670	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	97	from	elucidation	2522:2532	arg1	models					2684:2689	models	2684:2689	models of nociceptive and inflammatory pain	2684:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	98	theme	patients	2938:2945	arg1	treatment					2925:2933	the treatment	2921:2933	the treatment of patients with inflammatory and painful conditions	2921:2986	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	11	99	theme	chromatograph	1648:1660	arg1	fractionation					1662:1674	The chromatograph fractionation	1644:1674	The chromatograph fractionation of essential oil	1644:1691	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	13	100	theme	essential	1916:1924	arg1	oil					1926:1928	essential oil	1916:1928	essential oil (50 or 100 mg/kg, p.o.)	1916:1952	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	9	101	theme	antinociceptive	1402:1416	arg1	activity					1418:1425	the antinociceptive activity	1398:1425	the antinociceptive activity	1398:1425	Mechanical allodynia induced by carrageenan and hot plate tests were employed to evaluate the antinociceptive activity.
37059251	10	102	located	found	1458:1462	arg2	myrcene					1507:1513	the monoterpenes myrcene	1490:1513	the monoterpenes myrcene (13.81%)	1490:1522	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	10	102	located	found	1458:1462	arg1	oil					1481:1483	the essential oil	1467:1483	the essential oil	1467:1483	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	10	102	located	found	1458:1462	arg2	RESULTS					1428:1434	RESULTS	1428:1434	RESULTS The main constituents found in the essential oil	1428:1483	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	18	103	from	medicine	2886:2893	arg1	treatment					2925:2933	the treatment	2921:2933	the treatment of patients with inflammatory and painful conditions	2921:2986	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	1	104	theme	ETHNOPHARMACOLOGICAL	128:147	arg1	RELEVANCE					149:157	ETHNOPHARMACOLOGICAL RELEVANCE	128:157	ETHNOPHARMACOLOGICAL RELEVANCE	128:157	ETHNOPHARMACOLOGICAL RELEVANCE Lippia lacunosa Mart.
37059251	18	105	theme	painful	2969:2975	arg1	conditions					2977:2986	painful conditions	2969:2986	painful conditions	2969:2986	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	4	106	theme	mango	397:401	arg1	aroma					403:407	a characteristic mango aroma	380:407	a characteristic mango aroma	380:407	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	18	107	from	phytopharmaceutical	2898:2916	arg1	treatment					2925:2933	the treatment	2921:2933	the treatment of patients with inflammatory and painful conditions	2921:2986	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	0	108	theme	lacunosa	82:89	arg1	effect					65:70	antinociceptive and anti-inflammatory effect	27:70	antinociceptive and anti-inflammatory effect of Lippia lacunosa	27:89	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	0	108	theme	lacunosa	82:89	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	18	109	theme	activity	2603:2610	arg1	CONCLUSIONS					2506:2516	CONCLUSIONS	2506:2516	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2506:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	109	theme	activity	2603:2610	arg1	demonstration					2582:2594	the demonstration	2578:2594	the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2578:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	109	theme	activity	2603:2610	arg1	elucidation					2522:2532	The elucidation	2518:2532	The elucidation of the composition of the essential oil	2518:2572	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	110	theme	ethnopharmacological	2774:2793	arg1	use					2795:2797	the ancient ethnopharmacological use	2762:2797	the ancient ethnopharmacological use	2762:2797	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	3	111	theme	de	315:316	arg1	"					326:326	"chá de pedestre"	310:326	"chá de pedestre"	310:326	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	3	111	theme	de	315:316	arg1	It					295:296	It	295:296	It	295:296	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	14	112	theme	mechanical	2053:2062	arg1	allodynia					2064:2072	mechanical allodynia	2053:2072	mechanical allodynia	2053:2072	The ethanolic extract (100 mg/kg) reduced mechanical allodynia only in the 2 nd h of evaluation.
37059251	16	113	theme	hexane	2307:2312	arg1	extract					2314:2320	The hexane extract	2303:2320	The hexane extract	2303:2320	The hexane extract, essential oil, and majority fraction F33 also reduced the heat-induced nociceptive response.
37059251	18	114	theme	pain	2723:2726	arg1	models					2684:2689	models	2684:2689	models of nociceptive and inflammatory pain	2684:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	8	115	theme	paw	1234:1236	arg1	edema					1238:1242	Carrageenan-induced paw edema	1214:1242	Carrageenan-induced paw edema	1214:1242	Carrageenan-induced paw edema was used to investigate the anti-inflammatory activity in mice.
37059251	15	116	theme	other	2115:2119	arg1	hand					2121:2124	the other hand	2111:2124	the other hand	2111:2124	On the other hand, the hexane extract (50 or 100 mg/kg) and essential oil (100 mg/kg), as well as the majority fraction (10 mg/kg), reduced mechanical allodynia throughout the evaluation period.
37059251	13	117	dep	fraction	1966:1973	arg1	p.o.					1986:1989	p.o.	1986:1989	p.o.	1986:1989	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	13	117	dep	fraction	1966:1973	arg1	10 mg/kg					1976:1983	10 mg/kg	1976:1983	10 mg/kg	1976:1983	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	10	118	dep	RESULTS	1428:1434	arg1	constituents					1445:1456	The main constituents	1436:1456	RESULTS The main constituents found in the essential oil	1428:1483	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	3	119	from	"	343:343	arg1	medicine					353:360	folk medicine	348:360	folk medicine	348:360	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	12	120	dep	600 μg	1855:1860	arg1	models					1786:1791	experimental models	1773:1791	experimental models of paw edema and mechanical allodynia induced by carrageenan	1773:1852	In experimental models of paw edema and mechanical allodynia induced by carrageenan (600 μg, 30 μL, i.pl.)
37059251	2	121	from	Serra	220:224	arg1	plant					205:209	an endemic plant	194:209	an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil	194:292	& Schauer is an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil.
37059251	2	121	from	Serra	220:224	arg1	Schauer					183:189	Schauer	183:189	Schauer	183:189	& Schauer is an endemic plant from the Serra do Espinhaço mountain range located on the Atlantic plateau, Brazil.
37059251	16	122	theme	nociceptive	2394:2404	arg1	response					2406:2413	the heat-induced nociceptive response	2377:2413	the heat-induced nociceptive response	2377:2413	The hexane extract, essential oil, and majority fraction F33 also reduced the heat-induced nociceptive response.
37059251	6	123	from	oil	918:920	arg1	mice					940:943	mice	940:943	mice	940:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	14	124	theme	ethanolic	2015:2023	arg1	100 mg/kg					2034:2042	100 mg/kg	2034:2042	100 mg/kg	2034:2042	The ethanolic extract (100 mg/kg) reduced mechanical allodynia only in the 2 nd h of evaluation.
37059251	14	124	theme	ethanolic	2015:2023	arg1	extract					2025:2031	The ethanolic extract	2011:2031	The ethanolic extract (100 mg/kg)	2011:2043	The ethanolic extract (100 mg/kg) reduced mechanical allodynia only in the 2 nd h of evaluation.
37059251	18	125	theme	oil	2570:2572	arg1	composition					2541:2551	the composition	2537:2551	the composition of the essential oil	2537:2572	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	3	126	theme	folk	348:351	arg1	medicine					353:360	folk medicine	348:360	folk medicine	348:360	It is known as "chá de pedestre" and "rosmaninho" in folk medicine.
37059251	8	127	used	used	1248:1251	arg2	edema					1238:1242	Carrageenan-induced paw edema	1214:1242	Carrageenan-induced paw edema	1214:1242	Carrageenan-induced paw edema was used to investigate the anti-inflammatory activity in mice.
37059251	0	128	theme	antinociceptive	27:41	arg1	effect					65:70	antinociceptive and anti-inflammatory effect	27:70	antinociceptive and anti-inflammatory effect of Lippia lacunosa	27:89	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	0	128	theme	antinociceptive	27:41	arg1	characterization					9:24	Chemical characterization	0:24	Chemical characterization	0:24	Chemical characterization, antinociceptive and anti-inflammatory effect of Lippia lacunosa, a species used by the Bandeirantes.
37059251	7	129	theme	Ultra-Performance	1076:1092	arg1	UPLC					1117:1120	UPLC	1117:1120	UPLC	1117:1120	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	129	theme	Ultra-Performance	1076:1092	arg1	Chromatography					1101:1114	Ultra-Performance Liquid Chromatography	1076:1114	Ultra-Performance Liquid Chromatography (UPLC)	1076:1121	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	18	130	theme	species	2852:2858	arg1	evaluation					2834:2843	the evaluation	2830:2843	the evaluation of the species	2830:2858	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	5	131	dep	confused	551:558	arg1	used					581:584	used	581:584	used interchangeably with L. rotundifolia and L. pseudothea	581:639	It is often confused with and, therefore, used interchangeably with L. rotundifolia and L. pseudothea.
37059251	4	132	theme	sinus	462:466	arg1	infections					468:477	sinus infections	462:477	sinus infections	462:477	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	18	133	from	models	2643:2648	arg1	CONCLUSIONS					2506:2516	CONCLUSIONS	2506:2516	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2506:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	133	from	models	2643:2648	arg1	demonstration					2582:2594	the demonstration	2578:2594	the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain	2578:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	133	from	models	2643:2648	arg1	elucidation					2522:2532	The elucidation	2518:2532	The elucidation of the composition of the essential oil	2518:2572	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	134	theme	experimental	2630:2641	arg1	models					2643:2648	experimental models	2630:2648	experimental models of acute inflammation	2630:2670	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	4	135	used	used	423:426	arg2	species					368:374	This species	363:374	This species	363:374	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	13	136	theme	majority	1957:1964	arg1	fraction					1966:1973	majority fraction	1957:1973	majority fraction (10 mg/kg, p.o.)	1957:1990	, the administration of hexane extract, essential oil (50 or 100 mg/kg, p.o.) or majority fraction (10 mg/kg, p.o.) reduced paw edema.
37059251	7	137	theme	Thin	1181:1184	arg1	TLC					1208:1210	TLC	1208:1210	TLC	1208:1210	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	7	137	theme	Thin	1181:1184	arg1	Chromatography					1192:1205	Thin Layer Chromatography	1181:1205	Thin Layer Chromatography (TLC)	1181:1211	MATERIALS AND METHODS The chemical profile of L. lacunosa extracts and fractions were obtained by chromatographic methods such as Ultra-Performance Liquid Chromatography (UPLC), Gas Chromatography (GC), Column Chromatography (CC), and Thin Layer Chromatography (TLC).
37059251	6	138	theme	STUDY	653:657	arg1	AIM					642:644	AIM	642:644	AIM OF THE STUDY	642:657	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	11	139	theme	oil	1689:1691	arg1	fractionation					1662:1674	The chromatograph fractionation	1644:1674	The chromatograph fractionation of essential oil	1644:1691	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	6	140	theme	Lippia	744:749	arg1	lacunosa					751:758	Lippia lacunosa	744:758	Lippia lacunosa	744:758	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	16	141	theme	fraction	2351:2358	arg1	F33					2360:2362	majority fraction F33	2342:2362	majority fraction F33	2342:2362	The hexane extract, essential oil, and majority fraction F33 also reduced the heat-induced nociceptive response.
37059251	4	142	theme	long	527:530	arg1	walks					532:536	long walks	527:536	long walks	527:536	This species has a characteristic mango aroma and is widely used by the population for flu, colds, sinus infections, coughing, relaxing baths, and foot baths after long walks.
37059251	6	143	from	hexane	877:882	arg1	mice					940:943	mice	940:943	mice	940:943	AIM OF THE STUDY This study aimed to increase scientific knowledge on the ethnopharmacological use of Lippia lacunosa through the evaluation of the micromolecular composition and anti-inflammatory and antinociceptive activities of the hexane and ethanolic extracts, essential oil, and fractions in mice.
37059251	18	144	from	activity	2603:2610	arg1	models					2643:2648	experimental models	2630:2648	experimental models of acute inflammation	2630:2670	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	18	144	from	activity	2603:2610	arg1	models					2684:2689	models	2684:2689	models of nociceptive and inflammatory pain	2684:2726	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37059251	16	145	theme	heat-induced	2381:2392	arg1	response					2406:2413	the heat-induced nociceptive response	2377:2413	the heat-induced nociceptive response	2377:2413	The hexane extract, essential oil, and majority fraction F33 also reduced the heat-induced nociceptive response.
37059251	10	146	theme	essential	1471:1479	arg1	oil					1481:1483	the essential oil	1467:1483	the essential oil	1467:1483	RESULTS The main constituents found in the essential oil were the monoterpenes myrcene (13.81%), linalool (6.84%), ipsenone (21.2%), and myrcenone (25.44%); and sesquiterpenes elemol (7.30%) and spathulenol (3.15%).
37059251	11	147	theme	rich	1712:1715	arg1	fraction					1703:1710	a fraction	1701:1710	a fraction rich in the main compounds (F33), ipsenone and mircenone	1701:1767	The chromatograph fractionation of essential oil yielded a fraction rich in the main compounds (F33), ipsenone and mircenone.
37059251	18	148	theme	ancient	2766:2772	arg1	use					2795:2797	the ancient ethnopharmacological use	2762:2797	the ancient ethnopharmacological use	2762:2797	CONCLUSIONS The elucidation of the composition of the essential oil and the demonstration of the activity of L. lacunosa in experimental models of acute inflammation and also in models of nociceptive and inflammatory pain can help to increase knowledge on the ancient ethnopharmacological use by the Bandeirantes, aiming at the evaluation of the species as a candidate for herbal medicine or phytopharmaceutical in the treatment of patients with inflammatory and painful conditions.
37392526	0	0	theme	seed	69:72	arg1	oil					74:76	seed oil	69:76	seed oil in Brassica napus	69:94	Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus.
37392526	3	1	theme	transcription	592:604	arg1	WRINKLED1					643:651	WRINKLED1	643:651	WRINKLED1 (WRI1)	643:658	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	3	1	theme	transcription	592:604	arg1	factors					606:612	Two key transcription factors	584:612	Two key transcription factors	584:612	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	3	1	theme	transcription	592:604	arg1	COTYLEDON1					621:630	LEAFY COTYLEDON1	615:630	LEAFY COTYLEDON1 (LEC1)	615:637	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	4	2	theme	starch	969:974	arg1	synthesis					976:984	starch synthesis	969:984	starch synthesis	969:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	1	3	theme	phytoglobin	124:134	arg1	function					112:119	the function	108:119	the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L.	108:205	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	6	4	theme	low-oil	1438:1444	arg1	counterparts					1446:1457	their low-oil counterparts	1432:1457	their low-oil counterparts	1432:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	2	5	theme	changes	514:520	arg1	lack					500:503	the lack	496:503	the lack of major changes in composition of fatty acids (FA), and key agronomic traits	496:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	1	6	from	phytoglobin	124:134	arg1	level					157:161	seed oil level	148:161	seed oil level	148:161	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	4	7	theme	FRUCTOSE	892:899	arg1	ALDOLASE					914:921	FRUCTOSE BISPHOSPHATE ALDOLASE	892:921	FRUCTOSE BISPHOSPHATE ALDOLASE (FPA)	892:927	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	7	theme	FRUCTOSE	892:899	arg1	FPA					924:926	FPA	924:926	FPA	924:926	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	3	8	theme	fatty	695:699	arg1	FA					708:709	FA	708:709	FA	708:709	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	3	8	theme	fatty	695:699	arg1	acids					701:705	fatty acids	695:705	fatty acids (FA)	695:710	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	2	9	theme	oil	457:459	arg1	value					473:477	the oil nutritional value	453:477	the oil nutritional value	453:477	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	6	10	theme	high-oil	1401:1408	arg1	genotypes					1410:1418	high-oil genotypes	1401:1418	high-oil genotypes relative to their low-oil counterparts	1401:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	2	11	theme	major	508:512	arg1	changes					514:520	major changes	508:520	major changes in composition of fatty acids (FA), and key agronomic traits	508:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	6	12	theme	BnPgb2	1289:1294	arg1	requirement					1274:1284	The requirement	1270:1284	The requirement of BnPgb2 for oil deposition	1270:1313	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	5	13	dep	enzymes	1120:1126	arg1	A					1136:1136	SUBUNIT A	1128:1136	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	2	14	from	lack	500:503	arg1	composition					525:535	composition	525:535	composition of fatty acids (FA)	525:555	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	2	14	from	lack	500:503	arg1	traits					576:581	key agronomic traits	562:581	key agronomic traits	562:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	6	15	theme	higher	1365:1370	arg1	levels					1372:1377	the higher levels	1361:1377	the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts	1361:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	3	16	theme	LEAFY	615:619	arg1	LEC1					633:636	LEC1	633:636	LEC1	633:636	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	3	16	theme	LEAFY	615:619	arg1	factors					606:612	Two key transcription factors	584:612	Two key transcription factors	584:612	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	3	16	theme	LEAFY	615:619	arg1	COTYLEDON1					621:630	LEAFY COTYLEDON1	615:630	LEAFY COTYLEDON1 (LEC1)	615:637	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	1	17	from	function	112:119	arg1	crop					184:187	the oil-producing crop Brassica napus L.	166:205	the oil-producing crop Brassica napus L.	166:205	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	1	17	from	function	112:119	arg1	level					157:161	seed oil level	148:161	seed oil level	148:161	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	2	18	from	traits	576:581	arg1	lack					500:503	the lack	496:503	the lack of major changes in composition of fatty acids (FA), and key agronomic traits	496:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	4	19	theme	metabolism	847:856	arg1	enzymes					828:834	several enzymes	820:834	several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis	820:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	19	theme	metabolism	847:856	arg1	PHOSPHORYLASE					999:1011	ADP-GLUCOSE PHOSPHORYLASE	987:1011	ADP-GLUCOSE PHOSPHORYLASE (AGPase)	987:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	6	20	from	BnPgb2	1382:1387	arg1	seeds					1392:1396	seeds	1392:1396	seeds of high-oil genotypes relative to their low-oil counterparts	1392:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	1	21	theme	cruciferin1	297:307	arg1	promoter					309:316	the cruciferin1 promoter	293:316	the cruciferin1 promoter	293:316	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	2	22	theme	acids	546:550	arg1	composition					525:535	composition	525:535	composition of fatty acids (FA)	525:555	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	2	22	theme	acids	546:550	arg1	traits					576:581	key agronomic traits	562:581	key agronomic traits	562:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	4	23	theme	sugar	1050:1054	arg1	mobilization					1056:1067	sugar mobilization	1050:1067	sugar mobilization for FA production	1050:1085	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	24	theme	KINASE	951:956	arg1	enzymes					828:834	several enzymes	820:834	several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis	820:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	24	theme	KINASE	951:956	arg1	PHOSPHORYLASE					999:1011	ADP-GLUCOSE PHOSPHORYLASE	987:1011	ADP-GLUCOSE PHOSPHORYLASE (AGPase)	987:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	0	25	theme	Brassica	81:88	arg1	napus					90:94	Brassica napus	81:94	Brassica napus	81:94	Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus.
37392526	2	26	theme	fatty	540:544	arg1	FA					553:554	FA	553:554	FA	553:554	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	2	26	theme	fatty	540:544	arg1	acids					546:550	fatty acids	540:550	fatty acids (FA)	540:555	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	6	27	theme	genotypes	1410:1418	arg1	seeds					1392:1396	seeds	1392:1396	seeds of high-oil genotypes relative to their low-oil counterparts	1392:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	5	28	theme	CARBOXYLASE	1152:1162	arg1	A					1136:1136	SUBUNIT A	1128:1136	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	2	29	theme	BnPgb2	338:343	arg1	Over-expression					319:333	Over-expression	319:333	Over-expression of BnPgb2	319:343	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	5	30	theme	BnPgb2	1262:1267	arg1	over-expression					1243:1257	the over-expression	1239:1257	the over-expression of BnPgb2	1239:1267	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	2	31	theme	agronomic	566:574	arg1	traits					576:581	key agronomic traits	562:581	key agronomic traits	562:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	4	32	theme	concomitant	795:805	arg1	induction					807:815	The concomitant induction	791:815	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase)	791:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	5	33	theme	MALONYL-CoA	1177:1187	arg1	A					1136:1136	SUBUNIT A	1128:1136	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	0	34	theme	Seed-specific	0:12	arg1	expression					14:23	Seed-specific expression	0:23	Seed-specific expression of the class 2 Phytoglobin (Pgb2)	0:57	Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus.
37392526	2	35	theme	key	562:564	arg1	traits					576:581	key agronomic traits	562:581	key agronomic traits	562:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	4	36	theme	FA	1073:1074	arg1	production					1076:1085	FA production	1073:1085	FA production	1073:1085	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	5	37	theme	biosynthetic	1107:1118	arg1	enzymes					1120:1126	The two plastid FA biosynthetic enzymes	1088:1126	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	1	38	theme	transgenic	221:230	arg1	plants					232:237	transgenic plants	221:237	transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter	221:316	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	6	39	theme	relative	1420:1427	arg1	genotypes					1410:1418	high-oil genotypes	1401:1418	high-oil genotypes relative to their low-oil counterparts	1401:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	4	40	theme	sucrose	839:845	arg1	metabolism					847:856	sucrose metabolism	839:856	sucrose metabolism	839:856	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	2	41	theme	oil	368:370	arg1	amount					358:363	the amount	354:363	the amount of oil, which showed a positive relationship with the level of BnPgb2,	354:434	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	2	41	theme	oil	368:370	arg1	oil					368:370	oil	368:370	oil	368:370	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	5	42	dep	A	1136:1136	arg1	MCAT					1207:1210	ACP TRANSACYLASE (MCAT)	1189:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	5	43	theme	plastid	1096:1102	arg1	enzymes					1120:1126	The two plastid FA biosynthetic enzymes	1088:1126	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	1	44	dep	crop	184:187	arg1	Brassica					189:196	Brassica	189:196	Brassica	189:196	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	3	45	theme	BnPgb2	761:766	arg1	seeds					784:788	BnPgb2 over-expressing seeds	761:788	BnPgb2 over-expressing seeds	761:788	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	1	46	theme	oil	153:155	arg1	level					157:161	seed oil level	148:161	seed oil level	148:161	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	2	47	from	changes	514:520	arg1	composition					525:535	composition	525:535	composition of fatty acids (FA)	525:555	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	2	47	from	changes	514:520	arg1	traits					576:581	key agronomic traits	562:581	key agronomic traits	562:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	1	48	dep	Brassica	189:196	arg1	L.					204:205	Brassica napus L.	189:205	the oil-producing crop Brassica napus L.	166:205	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	6	49	from	seeds	1392:1396	arg1	levels					1372:1377	the higher levels	1361:1377	the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts	1361:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	3	50	theme	over-expressing	768:782	arg1	seeds					784:788	BnPgb2 over-expressing seeds	761:788	BnPgb2 over-expressing seeds	761:788	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	0	51	theme	class	32:36	arg1	Pgb2					53:56	Pgb2	53:56	Pgb2	53:56	Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus.
37392526	0	51	theme	class	32:36	arg1	Phytoglobin					40:50	the class 2 Phytoglobin	28:50	the class 2 Phytoglobin (Pgb2)	28:57	Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus.
37392526	4	52	theme	SUCROSE	859:865	arg1	SUS					878:880	SUS	878:880	SUS	878:880	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	52	theme	SUCROSE	859:865	arg1	SYNTHASE1					867:875	SUCROSE SYNTHASE1 (SUS) 1 and 3	859:889	SYNTHASE1	867:875	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	2	53	theme	nutritional	461:471	arg1	value					473:477	the oil nutritional value	453:477	the oil nutritional value	453:477	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	5	54	theme	SUBUNIT	1128:1134	arg1	A					1136:1136	SUBUNIT A	1128:1136	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	1	55	from	level	157:161	arg1	function					112:119	the function	108:119	the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L.	108:205	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	4	56	theme	SYNTHASE1	867:875	arg1	enzymes					828:834	several enzymes	820:834	several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis	820:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	56	theme	SYNTHASE1	867:875	arg1	PHOSPHORYLASE					999:1011	ADP-GLUCOSE PHOSPHORYLASE	987:1011	ADP-GLUCOSE PHOSPHORYLASE (AGPase)	987:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	2	57	from	composition	525:535	arg1	lack					500:503	the lack	496:503	the lack of major changes in composition of fatty acids (FA), and key agronomic traits	496:581	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	4	58	theme	several	820:826	arg1	enzymes					828:834	several enzymes	820:834	several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis	820:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	58	theme	several	820:826	arg1	PHOSPHORYLASE					999:1011	ADP-GLUCOSE PHOSPHORYLASE	987:1011	ADP-GLUCOSE PHOSPHORYLASE (AGPase)	987:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	59	theme	PHOSPHOGLYCERATE	934:949	arg1	PGK					959:961	PGK	959:961	PGK	959:961	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	59	theme	PHOSPHOGLYCERATE	934:949	arg1	KINASE					951:956	PHOSPHOGLYCERATE KINASE	934:956	PHOSPHOGLYCERATE KINASE (PGK)	934:962	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	6	60	theme	BnPgb2	1382:1387	arg1	levels					1372:1377	the higher levels	1361:1377	the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts	1361:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	0	61	theme	Phytoglobin	40:50	arg1	expression					14:23	Seed-specific expression	0:23	Seed-specific expression of the class 2 Phytoglobin (Pgb2)	0:57	Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus.
37392526	6	62	theme	natural	1340:1346	arg1	germplasm					1348:1356	natural germplasm	1340:1356	natural germplasm	1340:1356	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	2	63	theme	positive	388:395	arg1	relationship					397:408	a positive relationship	386:408	a positive relationship with the level of BnPgb2	386:433	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	5	64	theme	ACP	1189:1191	arg1	MCAT					1207:1210	ACP TRANSACYLASE (MCAT)	1189:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	6	65	from	levels	1372:1377	arg1	seeds					1392:1396	seeds	1392:1396	seeds of high-oil genotypes relative to their low-oil counterparts	1392:1457	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	4	66	theme	ALDOLASE	914:921	arg1	enzymes					828:834	several enzymes	820:834	several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis	820:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	66	theme	ALDOLASE	914:921	arg1	PHOSPHORYLASE					999:1011	ADP-GLUCOSE PHOSPHORYLASE	987:1011	ADP-GLUCOSE PHOSPHORYLASE (AGPase)	987:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	5	67	theme	TRANSACYLASE	1193:1204	arg1	MCAT					1207:1210	ACP TRANSACYLASE (MCAT)	1189:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	4	68	theme	BISPHOSPHATE	901:912	arg1	ALDOLASE					914:921	FRUCTOSE BISPHOSPHATE ALDOLASE	892:921	FRUCTOSE BISPHOSPHATE ALDOLASE (FPA)	892:927	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	68	theme	BISPHOSPHATE	901:912	arg1	FPA					924:926	FPA	924:926	FPA	924:926	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	6	69	theme	oil	1300:1302	arg1	deposition					1304:1313	oil deposition	1300:1313	oil deposition	1300:1313	The requirement of BnPgb2 for oil deposition was further evidenced in natural germplasm by the higher levels of BnPgb2 in seeds of high-oil genotypes relative to their low-oil counterparts.
37392526	4	70	theme	ADP-GLUCOSE	987:997	arg1	enzymes					828:834	several enzymes	820:834	several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis	820:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	70	theme	ADP-GLUCOSE	987:997	arg1	AGPase					1014:1019	AGPase	1014:1019	AGPase	1014:1019	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	70	theme	ADP-GLUCOSE	987:997	arg1	PHOSPHORYLASE					999:1011	ADP-GLUCOSE PHOSPHORYLASE	987:1011	ADP-GLUCOSE PHOSPHORYLASE (AGPase)	987:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	2	71	with	relationship	397:408	arg1	level					419:423	the level	415:423	the level of BnPgb2	415:433	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	5	72	theme	ACETYL-CoA	1141:1150	arg1	ACCA2					1165:1169	ACCA2	1165:1169	ACCA2	1165:1169	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	5	72	theme	ACETYL-CoA	1141:1150	arg1	CARBOXYLASE					1152:1162	ACETYL-CoA CARBOXYLASE	1141:1162	ACETYL-CoA CARBOXYLASE (ACCA2)	1141:1170	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	3	73	theme	acids	701:705	arg1	synthesis					682:690	the synthesis	678:690	the synthesis of fatty acids (FA)	678:710	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	1	74	theme	oil-producing	170:182	arg1	crop					184:187	the oil-producing crop Brassica napus L.	166:205	the oil-producing crop Brassica napus L.	166:205	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	4	75	theme	enzymes	828:834	arg1	induction					807:815	The concomitant induction	791:815	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase)	791:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	0	76	from	oil	74:76	arg1	napus					90:94	Brassica napus	81:94	Brassica napus	81:94	Seed-specific expression of the class 2 Phytoglobin (Pgb2) increases seed oil in Brassica napus.
37392526	1	77	theme	seed	148:151	arg1	level					157:161	seed oil level	148:161	seed oil level	148:161	To examine the function of phytoglobin 2 (Pgb2) on seed oil level in the oil-producing crop Brassica napus L., we generated transgenic plants in which BnPgb2 was over-expressed in the seeds using the cruciferin1 promoter.
37392526	5	78	theme	FA	1104:1105	arg1	enzymes					1120:1126	The two plastid FA biosynthetic enzymes	1088:1126	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT)	1088:1211	The two plastid FA biosynthetic enzymes SUBUNIT A OF ACETYL-CoA CARBOXYLASE (ACCA2), and MALONYL-CoA:ACP TRANSACYLASE (MCAT) were also up-regulated by the over-expression of BnPgb2.
37392526	2	79	theme	BnPgb2	428:433	arg1	level					419:423	the level	415:423	the level of BnPgb2	415:433	Over-expression of BnPgb2 elevated the amount of oil, which showed a positive relationship with the level of BnPgb2, without altering the oil nutritional value, as evidenced by the lack of major changes in composition of fatty acids (FA), and key agronomic traits.
37392526	3	80	theme	key	588:590	arg1	WRINKLED1					643:651	WRINKLED1	643:651	WRINKLED1 (WRI1)	643:658	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	3	80	theme	key	588:590	arg1	factors					606:612	Two key transcription factors	584:612	Two key transcription factors	584:612	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	3	80	theme	key	588:590	arg1	COTYLEDON1					621:630	LEAFY COTYLEDON1	615:630	LEAFY COTYLEDON1 (LEC1)	615:637	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
37392526	4	81	theme	synthesis	976:984	arg1	enzymes					828:834	several enzymes	820:834	several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis	820:984	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	4	81	theme	synthesis	976:984	arg1	PHOSPHORYLASE					999:1011	ADP-GLUCOSE PHOSPHORYLASE	987:1011	ADP-GLUCOSE PHOSPHORYLASE (AGPase)	987:1020	The concomitant induction of several enzymes of sucrose metabolism, SUCROSE SYNTHASE1 (SUS) 1 and 3, FRUCTOSE BISPHOSPHATE ALDOLASE (FPA), and PHOSPHOGLYCERATE KINASE (PGK), and starch synthesis, ADP-GLUCOSE PHOSPHORYLASE (AGPase) suggests that BnPgb2 favors sugar mobilization for FA production.
37392526	3	82	theme	oil	727:729	arg1	accumulation					731:742	oil accumulation	727:742	oil accumulation	727:742	Two key transcription factors, LEAFY COTYLEDON1 (LEC1) and WRINKLED1 (WRI1), known to promote the synthesis of fatty acids (FA) and potentiate oil accumulation, were induced in BnPgb2 over-expressing seeds.
35113795	6	0	theme	chromo-limulus	811:824	arg1	assay					844:848	chromo-limulus amoebocyte lysate assay	811:848	chromo-limulus amoebocyte lysate assay	811:848	Lipopolysaccharide was evaluated in fecal extract via chromo-limulus amoebocyte lysate assay.
35113795	2	1	theme	nonobese	318:325	arg1	horses					327:332	12 nonobese horses	315:332	12 nonobese horses (body condition score 4 to 5/9)	315:364	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	8	2	theme	murine	1218:1223	arg1	macrophages					1225:1235	murine macrophages	1218:1235	murine macrophages	1218:1235	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	5	3	theme	cytokine	543:550	arg1	expression					618:627	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression	530:627	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression	530:627	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	5	4	dep	cytokine	543:550	arg1	interleukin-6					598:610	interleukin-6	598:610	interleukin-6	598:610	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	5	4	dep	cytokine	543:550	arg1	interleukin-1β					553:566	interleukin-1β	553:566	interleukin-1β	553:566	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	5	4	dep	cytokine	543:550	arg1	factor-α					584:591	tumor necrosis factor-α	569:591	tumor necrosis factor-α	569:591	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	9	5	theme	inflammatory	1476:1487	arg1	response					1489:1496	an inflammatory response	1473:1496	an inflammatory response within the gastrointestinal tract of obese horses	1473:1546	Overall, this study showed that the microbiome may play a role in mediating an inflammatory response within the gastrointestinal tract of obese horses.
35113795	11	6	theme	therapeutic	1730:1740	arg1	interventions					1742:1754	potential diagnostic and therapeutic interventions	1705:1754	potential diagnostic and therapeutic interventions	1705:1754	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	5	7	theme	real-time	648:656	arg1	PCR					693:695	real-time quantitative reverse transcription PCR	648:695	real-time quantitative reverse transcription PCR assay	648:701	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	2	8	theme	score	298:302	arg1	horses					275:280	12 obese horses	266:280	12 obese horses (body condition score ≥ 7/9)	266:309	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	2	8	theme	score	298:302	arg1	7/9					306:308	body condition score ≥ 7/9	283:308	body condition score ≥ 7/9	283:308	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	9	9	theme	gastrointestinal	1509:1524	arg1	tract					1526:1530	the gastrointestinal tract	1505:1530	the gastrointestinal tract of obese horses	1505:1546	Overall, this study showed that the microbiome may play a role in mediating an inflammatory response within the gastrointestinal tract of obese horses.
35113795	8	10	theme	obese	1258:1262	arg1	horses					1264:1269	obese horses	1258:1269	obese horses	1258:1269	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	8	11	theme	microbial	1327:1335	arg1	composition					1337:1347	the enteric microbial composition	1315:1347	the enteric microbial composition of these horses	1315:1363	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	8	12	dep	RELEVANCE	1152:1160	arg1	levels					1176:1181	The increased levels	1162:1181	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro	1143:1278	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	5	13	theme	PCR	693:695	arg1	assay					697:701	real-time quantitative reverse transcription PCR assay	648:701	real-time quantitative reverse transcription PCR assay	648:701	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	1	14	theme	horses	209:214	arg1	microbiome					189:198	the enteric microbiome	177:198	the enteric microbiome of obese horses versus nonobese horses	177:237	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	2	15	theme	body	283:286	arg1	horses					275:280	12 obese horses	266:280	12 obese horses (body condition score ≥ 7/9)	266:309	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	2	15	theme	body	283:286	arg1	7/9					306:308	body condition score ≥ 7/9	283:308	body condition score ≥ 7/9	283:308	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	7	16	theme	proinflammatory	1040:1054	arg1	interleukin-1β					1066:1079	interleukin-1β	1066:1079	interleukin-1β	1066:1079	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	16	theme	proinflammatory	1040:1054	arg1	interleukin-6					1111:1123	interleukin-6	1111:1123	interleukin-6	1111:1123	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	16	theme	proinflammatory	1040:1054	arg1	factor-α					1097:1104	tumor necrosis factor-α	1082:1104	tumor necrosis factor-α	1082:1104	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	16	theme	proinflammatory	1040:1054	arg1	cytokines					1056:1064	the proinflammatory cytokines	1036:1064	the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6	1036:1123	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	9	17	theme	horses	1541:1546	arg1	tract					1526:1530	the gastrointestinal tract	1505:1530	the gastrointestinal tract of obese horses	1505:1546	Overall, this study showed that the microbiome may play a role in mediating an inflammatory response within the gastrointestinal tract of obese horses.
35113795	8	18	theme	horses	1358:1363	arg1	composition					1337:1347	the enteric microbial composition	1315:1347	the enteric microbial composition of these horses	1315:1363	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	7	19	theme	necrosis	1088:1095	arg1	factor-α					1097:1104	tumor necrosis factor-α	1082:1104	tumor necrosis factor-α	1082:1104	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	19	theme	necrosis	1088:1095	arg1	cytokines					1056:1064	the proinflammatory cytokines	1036:1064	the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6	1036:1123	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	8	20	from	differences	1300:1310	arg1	composition					1337:1347	the enteric microbial composition	1315:1347	the enteric microbial composition of these horses	1315:1363	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	2	21	with	samples	253:259	arg1	management					387:396	similar dietary management	371:396	similar dietary management	371:396	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	5	22	theme	reverse	671:677	arg1	PCR					693:695	real-time quantitative reverse transcription PCR	648:695	real-time quantitative reverse transcription PCR assay	648:701	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	7	23	theme	heightened	1011:1020	arg1	expression					1022:1031	a heightened expression	1009:1031	a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages	1009:1140	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	1	24	theme	inflammatory	122:133	arg1	response					135:142	the inflammatory response	118:142	the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses	118:237	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	8	25	theme	nonobese	1380:1387	arg1	horses					1389:1394	nonobese horses	1380:1394	nonobese horses	1380:1394	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	2	26	theme	SAMPLE	240:245	arg1	samples					253:259	SAMPLE Fecal samples	240:259	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.	240:397	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	0	27	theme	murine	68:73	arg1	macrophages					75:85	murine macrophages	68:85	murine macrophages	68:85	Fecal extract from obese horses induces an inflammatory response by murine macrophages in vitro.
35113795	7	28	theme	lipopolysaccharide	978:995	arg1	concentrations					960:973	higher concentrations	953:973	higher concentrations of lipopolysaccharide	953:995	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	1	29	theme	macrophages	154:164	arg1	response					135:142	the inflammatory response	118:142	the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses	118:237	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	2	30	dep	horses	327:332	arg1	score					350:354	body condition score	335:354	body condition score 4 to 5/9	335:363	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	5	31	theme	cytokine	708:715	arg1	concentration					717:729	cytokine concentration	708:729	cytokine concentration	708:729	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	5	32	theme	necrosis	575:582	arg1	factor-α					584:591	tumor necrosis factor-α	569:591	tumor necrosis factor-α	569:591	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	5	33	theme	Inflammatory	530:541	arg1	cytokine					543:550	Inflammatory cytokine	530:550	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression	530:627	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	0	34	from	horses	25:30	arg1	extract					6:12	Fecal extract	0:12	Fecal extract from obese horses	0:30	Fecal extract from obese horses induces an inflammatory response by murine macrophages in vitro.
35113795	7	35	dep	cytokines	1056:1064	arg1	interleukin-1β					1066:1079	interleukin-1β	1066:1079	interleukin-1β	1066:1079	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	35	dep	cytokines	1056:1064	arg1	cytokines					1056:1064	the proinflammatory cytokines	1036:1064	the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6	1036:1123	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	35	dep	cytokines	1056:1064	arg1	factor-α					1097:1104	tumor necrosis factor-α	1082:1104	tumor necrosis factor-α	1082:1104	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	35	dep	cytokines	1056:1064	arg1	interleukin-6					1111:1123	interleukin-6	1111:1123	interleukin-6	1111:1123	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	0	36	theme	Fecal	0:4	arg1	extract					6:12	Fecal extract	0:12	Fecal extract from obese horses	0:30	Fecal extract from obese horses induces an inflammatory response by murine macrophages in vitro.
35113795	7	37	theme	obese	930:934	arg1	horses					936:941	obese horses	930:941	obese horses	930:941	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	38	from	macrophages	1130:1140	arg1	expression					1022:1031	a heightened expression	1009:1031	a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages	1009:1140	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	10	39	theme	obesity	1563:1569	arg1	Mechanisms					1549:1558	Mechanisms	1549:1558	Mechanisms of obesity in the horse	1549:1582	Mechanisms of obesity in the horse have not been fully elucidated.
35113795	2	40	theme	similar	371:377	arg1	management					387:396	similar dietary management	371:396	similar dietary management	371:396	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	3	41	theme	Fecal	410:414	arg1	supernatant					416:426	PROCEDURES Fecal supernatant	399:426	PROCEDURES Fecal supernatant	399:426	PROCEDURES Fecal supernatant was prepared from frozen fecal samples.
35113795	11	42	theme	disease	1665:1671	arg1	pathophysiology					1646:1660	the pathophysiology	1642:1660	the pathophysiology of disease	1642:1671	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	3	43	theme	fecal	453:457	arg1	samples					459:465	frozen fecal samples	446:465	frozen fecal samples	446:465	PROCEDURES Fecal supernatant was prepared from frozen fecal samples.
35113795	11	44	theme	pathophysiology	1646:1660	arg1	understanding					1625:1637	Improved understanding	1616:1637	Improved understanding of the pathophysiology of disease	1616:1671	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	8	45	theme	increased	1166:1174	arg1	levels					1176:1181	The increased levels	1162:1181	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro	1143:1278	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	6	46	theme	lysate	837:842	arg1	assay					844:848	chromo-limulus amoebocyte lysate assay	811:848	chromo-limulus amoebocyte lysate assay	811:848	Lipopolysaccharide was evaluated in fecal extract via chromo-limulus amoebocyte lysate assay.
35113795	2	47	theme	body	335:338	arg1	score					350:354	body condition score	335:354	body condition score 4 to 5/9	335:363	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	7	48	theme	fecal	910:914	arg1	extracts					916:923	fecal extracts	910:923	fecal extracts from obese horses	910:941	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	1	49	theme	horses	232:237	arg1	microbiome					189:198	the enteric microbiome	177:198	the enteric microbiome of obese horses versus nonobese horses	177:237	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	5	50	theme	gene	613:616	arg1	expression					618:627	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression	530:627	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression	530:627	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	8	51	theme	markers	1199:1205	arg1	RELEVANCE					1152:1160	CLINICAL RELEVANCE The increased levels	1143:1181	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro	1143:1278	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	11	52	theme	potential	1705:1713	arg1	interventions					1742:1754	potential diagnostic and therapeutic interventions	1705:1754	potential diagnostic and therapeutic interventions	1705:1754	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	11	53	theme	equine	1760:1765	arg1	obesity					1767:1773	equine obesity	1760:1773	equine obesity	1760:1773	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	6	54	theme	fecal	793:797	arg1	extract					799:805	fecal extract	793:805	fecal extract	793:805	Lipopolysaccharide was evaluated in fecal extract via chromo-limulus amoebocyte lysate assay.
35113795	2	55	theme	obese	269:273	arg1	horses					275:280	12 obese horses	266:280	12 obese horses (body condition score ≥ 7/9)	266:309	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	2	55	theme	obese	269:273	arg1	7/9					306:308	body condition score ≥ 7/9	283:308	body condition score ≥ 7/9	283:308	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	7	56	theme	fecal	873:877	arg1	extracts					879:886	fecal extracts	873:886	fecal extracts from nonobese horses	873:907	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	2	57	from	horses	327:332	arg1	samples					253:259	SAMPLE Fecal samples	240:259	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.	240:397	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	8	58	theme	important	1290:1298	arg1	differences					1300:1310	important differences	1290:1310	important differences in the enteric microbial composition of these horses	1290:1363	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	2	59	theme	≥	304:304	arg1	horses					275:280	12 obese horses	266:280	12 obese horses (body condition score ≥ 7/9)	266:309	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	2	59	theme	≥	304:304	arg1	7/9					306:308	body condition score ≥ 7/9	283:308	body condition score ≥ 7/9	283:308	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	5	60	theme	transcription	679:691	arg1	PCR					693:695	real-time quantitative reverse transcription PCR	648:695	real-time quantitative reverse transcription PCR assay	648:701	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	11	61	theme	Improved	1616:1623	arg1	understanding					1625:1637	Improved understanding	1616:1637	Improved understanding of the pathophysiology of disease	1616:1671	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	2	62	theme	condition	288:296	arg1	horses					275:280	12 obese horses	266:280	12 obese horses (body condition score ≥ 7/9)	266:309	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	2	62	theme	condition	288:296	arg1	7/9					306:308	body condition score ≥ 7/9	283:308	body condition score ≥ 7/9	283:308	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	8	63	theme	enteric	1319:1325	arg1	composition					1337:1347	the enteric microbial composition	1315:1347	the enteric microbial composition of these horses	1315:1363	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	1	64	theme	enteric	181:187	arg1	microbiome					189:198	the enteric microbiome	177:198	the enteric microbiome of obese horses versus nonobese horses	177:237	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	1	65	theme	obese	203:207	arg1	horses					209:214	obese horses	203:214	obese horses	203:214	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	8	66	theme	horses	1264:1269	arg1	microbiome					1244:1253	the microbiome	1240:1253	the microbiome of obese horses in vitro	1240:1278	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	7	67	theme	tumor	1082:1086	arg1	factor-α					1097:1104	tumor necrosis factor-α	1082:1104	tumor necrosis factor-α	1082:1104	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	67	theme	tumor	1082:1086	arg1	cytokines					1056:1064	the proinflammatory cytokines	1036:1064	the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6	1036:1123	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	68	theme	cytokines	1056:1064	arg1	expression					1022:1031	a heightened expression	1009:1031	a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages	1009:1140	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	7	69	from	horses	936:941	arg1	extracts					916:923	fecal extracts	910:923	fecal extracts from obese horses	910:941	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	5	70	theme	quantitative	658:669	arg1	PCR					693:695	real-time quantitative reverse transcription PCR	648:695	real-time quantitative reverse transcription PCR assay	648:701	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	10	71	from	Mechanisms	1549:1558	arg1	horse					1578:1582	the horse	1574:1582	the horse	1574:1582	Mechanisms of obesity in the horse have not been fully elucidated.
35113795	2	72	theme	Fecal	247:251	arg1	samples					253:259	SAMPLE Fecal samples	240:259	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.	240:397	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	2	73	from	horses	275:280	arg1	samples					253:259	SAMPLE Fecal samples	240:259	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.	240:397	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	4	74	theme	RAW	468:470	arg1	cells					489:493	RAW 264.7 macrophage cells	468:493	RAW 264.7 macrophage cells	468:493	RAW 264.7 macrophage cells were exposed to the fecal extract.
35113795	1	75	theme	murine	147:152	arg1	macrophages					154:164	murine macrophages	147:164	murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses	147:237	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	4	76	theme	macrophage	478:487	arg1	cells					489:493	RAW 264.7 macrophage cells	468:493	RAW 264.7 macrophage cells	468:493	RAW 264.7 macrophage cells were exposed to the fecal extract.
35113795	5	77	theme	tumor	569:573	arg1	factor-α					584:591	tumor necrosis factor-α	569:591	tumor necrosis factor-α	569:591	Inflammatory cytokine (interleukin-1β, tumor necrosis factor-α, and interleukin-6) gene expression was quantified via real-time quantitative reverse transcription PCR assay, and cytokine concentration was quantified via ELISA.
35113795	7	78	from	horses	902:907	arg1	extracts					879:886	fecal extracts	873:886	fecal extracts from nonobese horses	873:907	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	3	79	theme	PROCEDURES	399:408	arg1	supernatant					416:426	PROCEDURES Fecal supernatant	399:426	PROCEDURES Fecal supernatant	399:426	PROCEDURES Fecal supernatant was prepared from frozen fecal samples.
35113795	7	80	theme	higher	953:958	arg1	concentrations					960:973	higher concentrations	953:973	higher concentrations of lipopolysaccharide	953:995	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
35113795	4	81	theme	fecal	515:519	arg1	extract					521:527	the fecal extract	511:527	the fecal extract	511:527	RAW 264.7 macrophage cells were exposed to the fecal extract.
35113795	2	82	theme	condition	340:348	arg1	score					350:354	body condition score	335:354	body condition score 4 to 5/9	335:363	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	0	83	theme	obese	19:23	arg1	horses					25:30	obese horses	19:30	obese horses	19:30	Fecal extract from obese horses induces an inflammatory response by murine macrophages in vitro.
35113795	11	84	dep	potential	1705:1713	arg1	diagnostic					1715:1724	diagnostic	1715:1724	diagnostic	1715:1724	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	2	85	theme	dietary	379:385	arg1	management					387:396	similar dietary management	371:396	similar dietary management	371:396	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	8	86	theme	CLINICAL	1143:1150	arg1	RELEVANCE					1152:1160	CLINICAL RELEVANCE The increased levels	1143:1181	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro	1143:1278	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	9	87	theme	obese	1535:1539	arg1	horses					1541:1546	obese horses	1535:1546	obese horses	1535:1546	Overall, this study showed that the microbiome may play a role in mediating an inflammatory response within the gastrointestinal tract of obese horses.
35113795	2	88	dep	5/9	361:363	arg1	to					358:359	to	358:359	to	358:359	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	2	89	theme	5/9	361:363	arg1	score					350:354	body condition score	335:354	body condition score 4 to 5/9	335:363	SAMPLE Fecal samples from 12 obese horses (body condition score ≥ 7/9) and 12 nonobese horses (body condition score 4 to 5/9) with similar dietary management.
35113795	0	90	theme	inflammatory	43:54	arg1	response					56:63	an inflammatory response	40:63	an inflammatory response by murine macrophages	40:85	Fecal extract from obese horses induces an inflammatory response by murine macrophages in vitro.
35113795	11	91	theme	future	1684:1689	arg1	research					1691:1698	future research	1684:1698	future research into potential diagnostic and therapeutic interventions for equine obesity	1684:1773	Improved understanding of the pathophysiology of disease will guide future research into potential diagnostic and therapeutic interventions for equine obesity.
35113795	3	92	theme	frozen	446:451	arg1	samples					459:465	frozen fecal samples	446:465	frozen fecal samples	446:465	PROCEDURES Fecal supernatant was prepared from frozen fecal samples.
35113795	1	93	theme	nonobese	223:230	arg1	horses					232:237	nonobese horses	223:237	nonobese horses	223:237	OBJECTIVE To compare the inflammatory response of murine macrophages exposed to the enteric microbiome of obese horses versus nonobese horses.
35113795	8	94	theme	inflammatory	1186:1197	arg1	markers					1199:1205	inflammatory markers	1186:1205	inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro	1186:1278	CLINICAL RELEVANCE The increased levels of inflammatory markers induced in murine macrophages by the microbiome of obese horses in vitro suggested important differences in the enteric microbial composition of these horses, compared with nonobese horses.
35113795	6	95	theme	amoebocyte	826:835	arg1	assay					844:848	chromo-limulus amoebocyte lysate assay	811:848	chromo-limulus amoebocyte lysate assay	811:848	Lipopolysaccharide was evaluated in fecal extract via chromo-limulus amoebocyte lysate assay.
35113795	7	96	theme	nonobese	893:900	arg1	horses					902:907	nonobese horses	893:907	nonobese horses	893:907	RESULTS Compared with fecal extracts from nonobese horses, fecal extracts from obese horses presented higher concentrations of lipopolysaccharide and induced a heightened expression of the proinflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-6 from macrophages.
37092745	5	0	theme	pregnant	578:585	arg1	rats					587:590	pregnant rats	578:590	pregnant rats	578:590	In this study, pregnant rats were induced by LPS to establish a rat model of preeclampsia.
37092745	1	1	theme	essential	157:165	arg1	cause					167:171	an essential cause	154:171	an essential cause of perinatal and neonatal death	154:203	Preeclampsia is a pregnancy-specific disease, which has become an essential cause of perinatal and neonatal death.
37092745	7	2	theme	protective	754:763	arg1	effect					765:770	the protective effect	750:770	the protective effect of vitamin D supplementation on LPS-preeclampsia rats	750:824	In addition, the protective effect of vitamin D supplementation on LPS-preeclampsia rats was evaluated.
37092745	3	3	theme	vitamin	348:354	arg1	supplementation					358:372	vitamin D supplementation	348:372	vitamin D supplementation	348:372	Epidemiological studies provide convincing evidence that vitamin D supplementation can prevent the onset of preeclampsia.
37092745	10	4	theme	Vitamin	1088:1094	arg1	supplementation					1098:1112	Vitamin D supplementation	1088:1112	Vitamin D supplementation	1088:1112	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	5	5	theme	rat	627:629	arg1	model					631:635	a rat model	625:635	a rat model of preeclampsia	625:651	In this study, pregnant rats were induced by LPS to establish a rat model of preeclampsia.
37092745	6	6	theme	16-sequence	654:664	arg1	analysis					666:673	16-sequence analysis	654:673	16-sequence analysis	654:673	16-sequence analysis was used to determine the composition of microflora in feces.
37092745	11	7	theme	therapeutic	1505:1515	arg1	mechanisms					1459:1468	vitamin D-mediated microbial mechanisms	1430:1468	vitamin D-mediated microbial mechanisms	1430:1468	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	7	theme	therapeutic	1505:1515	arg1	inhibition					1480:1489	their inhibition	1474:1489	their inhibition	1474:1489	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	7	theme	therapeutic	1505:1515	arg1	targets					1517:1523	potential therapeutic targets	1495:1523	potential therapeutic targets for the treatment of preeclampsia	1495:1557	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	10	8	dep	factors	1246:1252	arg1	LPS-induced					1254:1264	LPS-induced	1254:1264	LPS-induced	1254:1264	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	8	9	from	pressure	875:882	arg1	group					927:931	the LPS group	919:931	the LPS group	919:931	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	10	10	theme	D	1096:1096	arg1	supplementation					1098:1112	Vitamin D supplementation	1088:1112	Vitamin D supplementation	1088:1112	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	8	11	theme	rats	911:914	arg1	creatinine					888:897	creatinine	888:897	creatinine	888:897	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	8	11	theme	rats	911:914	arg1	pressure					875:882	blood pressure	869:882	blood pressure	869:882	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	10	12	theme	artery	1368:1373	arg1	remodeling					1375:1384	uterine spiral artery remodeling	1353:1384	uterine spiral artery remodeling induced by LPS	1353:1399	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	5	13	theme	preeclampsia	640:651	arg1	model					631:635	a rat model	625:635	a rat model of preeclampsia	625:651	In this study, pregnant rats were induced by LPS to establish a rat model of preeclampsia.
37092745	4	14	from	research	422:429	arg1	strategies					483:492	effective treatment strategies	463:492	effective treatment strategies	463:492	However, research on the microbial mechanisms and effective treatment strategies for placental inflammation induced by lipopolysaccharide is lacking.
37092745	4	14	from	research	422:429	arg1	mechanisms					448:457	the microbial mechanisms	434:457	the microbial mechanisms	434:457	However, research on the microbial mechanisms and effective treatment strategies for placental inflammation induced by lipopolysaccharide is lacking.
37092745	8	15	theme	pregnant	902:909	arg1	rats					911:914	pregnant rats	902:914	pregnant rats	902:914	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	10	16	theme	inflammatory	1233:1244	arg1	factors					1246:1252	inflammatory factors	1233:1252	inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway	1233:1307	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	2	17	theme	metabolic	272:280	arg1	pathway					282:288	the metabolic pathway	268:288	the metabolic pathway	268:288	Gut microflora becomes the regulator of host immunity through the metabolic pathway.
37092745	10	18	theme	uterine	1353:1359	arg1	remodeling					1375:1384	uterine spiral artery remodeling	1353:1384	uterine spiral artery remodeling induced by LPS	1353:1399	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	1	19	theme	perinatal	176:184	arg1	death					199:203	perinatal and neonatal death	176:203	perinatal and neonatal death	176:203	Preeclampsia is a pregnancy-specific disease, which has become an essential cause of perinatal and neonatal death.
37092745	10	20	theme	preeclampsia	1139:1150	arg1	symptoms					1127:1134	the symptoms	1123:1134	the symptoms of preeclampsia	1123:1150	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	0	21	theme	Vitamin	0:6	arg1	D					8:8	Vitamin D	0:8	Vitamin D	0:8	Vitamin D regulates microflora and ameliorates LPS-induced placental inflammation in rats.
37092745	10	22	theme	beneficial	1191:1200	arg1	flora					1202:1206	intestinal beneficial flora	1180:1206	intestinal beneficial flora	1180:1206	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	6	23	from	composition	701:711	arg1	feces					730:734	feces	730:734	feces	730:734	16-sequence analysis was used to determine the composition of microflora in feces.
37092745	1	24	theme	neonatal	190:197	arg1	death					199:203	perinatal and neonatal death	176:203	perinatal and neonatal death	176:203	Preeclampsia is a pregnancy-specific disease, which has become an essential cause of perinatal and neonatal death.
37092745	10	25	theme	intestinal	1180:1189	arg1	flora					1202:1206	intestinal beneficial flora	1180:1206	intestinal beneficial flora	1180:1206	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	6	26	theme	microflora	716:725	arg1	composition					701:711	the composition	697:711	the composition of microflora in feces	697:734	16-sequence analysis was used to determine the composition of microflora in feces.
37092745	1	27	theme	death	199:203	arg1	cause					167:171	an essential cause	154:171	an essential cause of perinatal and neonatal death	154:203	Preeclampsia is a pregnancy-specific disease, which has become an essential cause of perinatal and neonatal death.
37092745	3	28	theme	preeclampsia	399:410	arg1	onset					390:394	the onset	386:394	the onset of preeclampsia	386:410	Epidemiological studies provide convincing evidence that vitamin D supplementation can prevent the onset of preeclampsia.
37092745	3	29	theme	Epidemiological	291:305	arg1	studies					307:313	Epidemiological studies	291:313	Epidemiological studies	291:313	Epidemiological studies provide convincing evidence that vitamin D supplementation can prevent the onset of preeclampsia.
37092745	1	30	theme	pregnancy-specific	109:126	arg1	disease					128:134	a pregnancy-specific disease	107:134	a pregnancy-specific disease	107:134	Preeclampsia is a pregnancy-specific disease, which has become an essential cause of perinatal and neonatal death.
37092745	1	30	theme	pregnancy-specific	109:126	arg1	Preeclampsia					91:102	Preeclampsia	91:102	Preeclampsia	91:102	Preeclampsia is a pregnancy-specific disease, which has become an essential cause of perinatal and neonatal death.
37092745	11	31	theme	preeclampsia	1546:1557	arg1	treatment					1533:1541	the treatment	1529:1541	the treatment of preeclampsia	1529:1557	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	8	32	from	creatinine	888:897	arg1	group					927:931	the LPS group	919:931	the LPS group	919:931	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	11	33	theme	D-mediated	1438:1447	arg1	mechanisms					1459:1468	vitamin D-mediated microbial mechanisms	1430:1468	vitamin D-mediated microbial mechanisms	1430:1468	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	33	theme	D-mediated	1438:1447	arg1	inhibition					1480:1489	their inhibition	1474:1489	their inhibition	1474:1489	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	33	theme	D-mediated	1438:1447	arg1	targets					1517:1523	potential therapeutic targets	1495:1523	potential therapeutic targets for the treatment of preeclampsia	1495:1557	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	10	34	theme	spiral	1361:1366	arg1	remodeling					1375:1384	uterine spiral artery remodeling	1353:1384	uterine spiral artery remodeling induced by LPS	1353:1399	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	4	35	theme	treatment	473:481	arg1	strategies					483:492	effective treatment strategies	463:492	effective treatment strategies	463:492	However, research on the microbial mechanisms and effective treatment strategies for placental inflammation induced by lipopolysaccharide is lacking.
37092745	10	36	theme	remodeling	1375:1384	arg1	disturbance					1338:1348	the disturbance	1334:1348	the disturbance of uterine spiral artery remodeling induced by LPS	1334:1399	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	9	37	theme	intestinal	1024:1033	arg1	community					1045:1053	the intestinal microbial community	1020:1053	the intestinal microbial community	1020:1053	Additionally, LPS disturbed the intestinal microbial community and reduced microbial diversity.
37092745	7	38	from	effect	765:770	arg1	rats					821:824	LPS-preeclampsia rats	804:824	LPS-preeclampsia rats	804:824	In addition, the protective effect of vitamin D supplementation on LPS-preeclampsia rats was evaluated.
37092745	4	39	theme	placental	498:506	arg1	inflammation					508:519	placental inflammation	498:519	placental inflammation induced by lipopolysaccharide	498:549	However, research on the microbial mechanisms and effective treatment strategies for placental inflammation induced by lipopolysaccharide is lacking.
37092745	11	40	theme	potential	1495:1503	arg1	mechanisms					1459:1468	vitamin D-mediated microbial mechanisms	1430:1468	vitamin D-mediated microbial mechanisms	1430:1468	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	40	theme	potential	1495:1503	arg1	inhibition					1480:1489	their inhibition	1474:1489	their inhibition	1474:1489	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	40	theme	potential	1495:1503	arg1	targets					1517:1523	potential therapeutic targets	1495:1523	potential therapeutic targets for the treatment of preeclampsia	1495:1557	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	10	41	theme	flora	1202:1206	arg1	abundance					1167:1175	the abundance	1163:1175	the abundance of intestinal beneficial flora	1163:1206	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	0	42	theme	placental	59:67	arg1	inflammation					69:80	LPS-induced placental inflammation	47:80	LPS-induced placental inflammation in rats	47:88	Vitamin D regulates microflora and ameliorates LPS-induced placental inflammation in rats.
37092745	3	43	theme	convincing	323:332	arg1	evidence					334:341	convincing evidence that vitamin D supplementation can prevent the onset of preeclampsia	323:410	convincing evidence that vitamin D supplementation can prevent the onset of preeclampsia	323:410	Epidemiological studies provide convincing evidence that vitamin D supplementation can prevent the onset of preeclampsia.
37092745	0	44	from	inflammation	69:80	arg1	rats					85:88	rats	85:88	rats	85:88	Vitamin D regulates microflora and ameliorates LPS-induced placental inflammation in rats.
37092745	0	45	theme	LPS-induced	47:57	arg1	inflammation					69:80	LPS-induced placental inflammation	47:80	LPS-induced placental inflammation in rats	47:88	Vitamin D regulates microflora and ameliorates LPS-induced placental inflammation in rats.
37092745	4	46	theme	microbial	438:446	arg1	mechanisms					448:457	the microbial mechanisms	434:457	the microbial mechanisms	434:457	However, research on the microbial mechanisms and effective treatment strategies for placental inflammation induced by lipopolysaccharide is lacking.
37092745	9	47	theme	microbial	1067:1075	arg1	diversity					1077:1085	microbial diversity	1067:1085	microbial diversity	1067:1085	Additionally, LPS disturbed the intestinal microbial community and reduced microbial diversity.
37092745	7	48	theme	LPS-preeclampsia	804:819	arg1	rats					821:824	LPS-preeclampsia rats	804:824	LPS-preeclampsia rats	804:824	In addition, the protective effect of vitamin D supplementation on LPS-preeclampsia rats was evaluated.
37092745	2	49	theme	Gut	206:208	arg1	microflora					210:219	Gut microflora	206:219	Gut microflora	206:219	Gut microflora becomes the regulator of host immunity through the metabolic pathway.
37092745	6	50	used	used	679:682	arg2	analysis					666:673	16-sequence analysis	654:673	16-sequence analysis	654:673	16-sequence analysis was used to determine the composition of microflora in feces.
37092745	8	51	dep	pressure	875:882	arg1	the					865:867	the	865:867	the	865:867	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	7	52	theme	D	783:783	arg1	supplementation					785:799	vitamin D supplementation	775:799	vitamin D supplementation	775:799	In addition, the protective effect of vitamin D supplementation on LPS-preeclampsia rats was evaluated.
37092745	4	53	theme	effective	463:471	arg1	strategies					483:492	effective treatment strategies	463:492	effective treatment strategies	463:492	However, research on the microbial mechanisms and effective treatment strategies for placental inflammation induced by lipopolysaccharide is lacking.
37092745	8	54	theme	control	977:983	arg1	group					985:989	the control group	973:989	the control group	973:989	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	8	55	theme	LPS	923:925	arg1	group					927:931	the LPS group	919:931	the LPS group	919:931	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	8	56	theme	blood	869:873	arg1	pressure					875:882	blood pressure	869:882	blood pressure	869:882	The results showed that the blood pressure and creatinine of pregnant rats in the LPS group were significantly higher than those in the control group.
37092745	7	57	theme	supplementation	785:799	arg1	effect					765:770	the protective effect	750:770	the protective effect of vitamin D supplementation on LPS-preeclampsia rats	750:824	In addition, the protective effect of vitamin D supplementation on LPS-preeclampsia rats was evaluated.
37092745	3	58	theme	D	356:356	arg1	supplementation					358:372	vitamin D supplementation	348:372	vitamin D supplementation	348:372	Epidemiological studies provide convincing evidence that vitamin D supplementation can prevent the onset of preeclampsia.
37092745	2	59	theme	immunity	251:258	arg1	regulator					233:241	the regulator	229:241	the regulator of host immunity	229:258	Gut microflora becomes the regulator of host immunity through the metabolic pathway.
37092745	10	60	theme	TLR4/MYD88/NF-KB	1284:1299	arg1	pathway					1301:1307	the TLR4/MYD88/NF-KB pathway	1280:1307	the TLR4/MYD88/NF-KB pathway	1280:1307	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	9	61	theme	microbial	1035:1043	arg1	community					1045:1053	the intestinal microbial community	1020:1053	the intestinal microbial community	1020:1053	Additionally, LPS disturbed the intestinal microbial community and reduced microbial diversity.
37092745	11	62	theme	microbial	1449:1457	arg1	mechanisms					1459:1468	vitamin D-mediated microbial mechanisms	1430:1468	vitamin D-mediated microbial mechanisms	1430:1468	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	62	theme	microbial	1449:1457	arg1	inhibition					1480:1489	their inhibition	1474:1489	their inhibition	1474:1489	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	11	62	theme	microbial	1449:1457	arg1	targets					1517:1523	potential therapeutic targets	1495:1523	potential therapeutic targets for the treatment of preeclampsia	1495:1557	This study established that vitamin D-mediated microbial mechanisms and their inhibition are potential therapeutic targets for the treatment of preeclampsia.
37092745	2	63	theme	host	246:249	arg1	immunity					251:258	host immunity	246:258	host immunity	246:258	Gut microflora becomes the regulator of host immunity through the metabolic pathway.
37092745	10	64	theme	factors	1246:1252	arg1	level					1224:1228	the level	1220:1228	the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway	1220:1307	Vitamin D supplementation improves the symptoms of preeclampsia, increases the abundance of intestinal beneficial flora, normalizes the level of inflammatory factors LPS-induced by inhibiting the TLR4/MYD88/NF-KB pathway, and effectively resists the disturbance of uterine spiral artery remodeling induced by LPS.
37092745	7	65	theme	vitamin	775:781	arg1	supplementation					785:799	vitamin D supplementation	775:799	vitamin D supplementation	775:799	In addition, the protective effect of vitamin D supplementation on LPS-preeclampsia rats was evaluated.
37302746	0	0	theme	developing	84:93	arg1	brain					95:99	the developing brain	80:99	the developing brain	80:99	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	3	1	theme	benzodiazepine	706:719	arg1	sedation					721:728	benzodiazepine sedation	706:728	benzodiazepine sedation	706:728	In weanling rats, mild-moderate inflammation was induced with lipopolysaccharide (LPS) on postnatal day (P)18 and combined with 3 days repeated opioid and benzodiazepine sedation using morphine and midazolam (MorMdz) between P19-21.
37302746	6	2	dep	higher	1413:1418	arg1	Iba1					1453:1456	Iba1	1453:1456	Iba1	1453:1456	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	2	3	theme	multidrug	362:370	arg1	sedation					372:379	multidrug sedation	362:379	multidrug sedation with these medications	362:402	However, little is known about how multidrug sedation with these medications interacts with inflammation in the developing brain, a frequent condition during childhood critical illness that has not been extensively studied.
37302746	1	4	theme	opioids	260:266	arg1	use					230:232	the use	226:232	the use of benzodiazepines and/or opioids	226:266	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	1	4	theme	opioids	260:266	arg1	factors					277:283	risk factors	272:283	risk factors for delirium and post-discharge sequelae	272:324	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	6	5	theme	western	1356:1362	arg1	blots					1364:1368	western blots	1356:1368	western blots of P22 brain homogenate	1356:1392	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	3	6	theme	repeated	686:693	arg1	opioid					695:700	3 days repeated opioid	679:700	3 days repeated opioid	679:700	In weanling rats, mild-moderate inflammation was induced with lipopolysaccharide (LPS) on postnatal day (P)18 and combined with 3 days repeated opioid and benzodiazepine sedation using morphine and midazolam (MorMdz) between P19-21.
37302746	8	7	theme	sedation	1775:1782	arg1	effects					1754:1760	the effects	1750:1760	the effects of multidrug sedation on homeostatic neuroimmune responses	1750:1819	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	6	8	theme	adaptor	1284:1290	arg1	molecule					1292:1299	calcium-binding adaptor molecule 1	1268:1301	calcium-binding adaptor molecule 1 (Iba1)	1268:1308	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	8	theme	adaptor	1284:1290	arg1	Iba1					1304:1307	Iba1	1304:1307	Iba1	1304:1307	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	8	9	theme	multidrug	1765:1773	arg1	sedation					1775:1782	multidrug sedation	1765:1782	multidrug sedation	1765:1782	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	6	10	dep	Iba1	1453:1456	arg1	p < 0.0001					1459:1468	p < 0.0001	1459:1468	p < 0.0001	1459:1468	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	10	dep	Iba1	1453:1456	arg1	p < 0.001					1477:1485	p < 0.001	1477:1485	p < 0.001	1477:1485	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	10	dep	Iba1	1453:1456	arg1	GFAP					1471:1474	GFAP	1471:1474	GFAP	1471:1474	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	4	11	theme	z-score	1031:1037	arg1	composite					1039:1047	a z-score composite	1029:1047	a z-score composite	1029:1047	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	1	12	theme	risk	272:275	arg1	use					230:232	the use	226:232	the use of benzodiazepines and/or opioids	226:266	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	1	12	theme	risk	272:275	arg1	factors					277:283	risk factors	272:283	risk factors for delirium and post-discharge sequelae	272:324	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	4	13	theme	rat	941:943	arg1	pups					945:948	male and female rat pups	925:948	male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group)	925:1003	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	5	14	dep	compared	1134:1141	arg1	F3,78 = 38.1					1162:1173	F3,78 = 38.1	1162:1173	F3,78 = 38.1	1162:1173	Composite scores were significantly increased in LPS, MorMdz, and LPS/MorMdz groups compared to saline control (F3,78 = 38.1, p < 0.0001).
37302746	6	15	theme	calcium-binding	1268:1282	arg1	molecule					1292:1299	calcium-binding adaptor molecule 1	1268:1301	calcium-binding adaptor molecule 1 (Iba1)	1268:1308	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	15	theme	calcium-binding	1268:1282	arg1	Iba1					1304:1307	Iba1	1304:1307	Iba1	1304:1307	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	8	16	theme	homeostatic	1787:1797	arg1	responses					1811:1819	homeostatic neuroimmune responses	1787:1819	homeostatic neuroimmune responses	1787:1819	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	8	17	theme	potential	1662:1670	arg1	interest					1672:1679	potential interest	1662:1679	potential interest	1662:1679	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	4	18	theme	dog	864:866	arg1	shakes					868:873	wet dog shakes	860:873	wet dog shakes	860:873	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	4	19	theme	Delirium-like	784:796	arg1	shakes					868:873	wet dog shakes	860:873	wet dog shakes	860:873	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	4	19	theme	Delirium-like	784:796	arg1	delay					880:884	delay	880:884	delay in finding buried food	880:907	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	4	19	theme	Delirium-like	784:796	arg1	behaviors					798:806	Delirium-like behaviors	784:806	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food	784:907	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	4	19	theme	Delirium-like	784:796	arg1	response					827:834	abnormal response	818:834	abnormal response to whisker stimulation	818:857	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	6	20	theme	glial-associated	1217:1232	arg1	markers					1252:1258	glial-associated neuroinflammatory markers	1217:1258	glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate	1217:1392	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	0	21	theme	Potential	102:110	arg1	induction					112:120	Potential induction	102:120	Potential induction of delirium-like behaviors	102:147	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	0	22	from	context	42:48	arg1	brain					95:99	the developing brain	80:99	the developing brain	80:99	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	4	23	theme	wet	860:862	arg1	shakes					868:873	wet dog shakes	860:873	wet dog shakes	860:873	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	3	24	theme	weanling	554:561	arg1	rats					563:566	weanling rats	554:566	weanling rats	554:566	In weanling rats, mild-moderate inflammation was induced with lipopolysaccharide (LPS) on postnatal day (P)18 and combined with 3 days repeated opioid and benzodiazepine sedation using morphine and midazolam (MorMdz) between P19-21.
37302746	7	25	theme	Saline	1577:1582	arg1	brains					1543:1548	brains	1543:1548	brains of LPS-treated pups versus Saline (p = 0.002)	1543:1594	Likewise, proinflammatory cytokines were increased in brains of LPS-treated pups versus Saline (p = 0.002), but not LPS/MorMdz-treated pups (p = 0.16).
37302746	0	26	theme	sedative	14:21	arg1	effects					23:29	sedative effects	14:29	sedative effects	14:29	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	5	27	theme	LPS	1099:1101	arg1	MorMdz					1104:1109	LPS, MorMdz, and LPS/MorMdz groups	1099:1132	MorMdz	1104:1109	Composite scores were significantly increased in LPS, MorMdz, and LPS/MorMdz groups compared to saline control (F3,78 = 38.1, p < 0.0001).
37302746	6	28	theme	neuroinflammatory	1234:1250	arg1	markers					1252:1258	glial-associated neuroinflammatory markers	1217:1258	glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate	1217:1392	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	8	29	theme	neuroimmune	1799:1809	arg1	responses					1811:1819	homeostatic neuroimmune responses	1787:1819	homeostatic neuroimmune responses	1787:1819	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	4	30	theme	buried	897:902	arg1	food					904:907	buried food	897:907	buried food	897:907	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	1	31	theme	pediatric	194:202	arg1	illness					213:219	pediatric critical illness	194:219	pediatric critical illness	194:219	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	6	32	theme	fibrillary	1320:1329	arg1	GFAP					1347:1350	GFAP	1347:1350	GFAP	1347:1350	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	32	theme	fibrillary	1320:1329	arg1	protein					1338:1344	glial fibrillary acidic protein	1314:1344	glial fibrillary acidic protein (GFAP)	1314:1351	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	5	33	theme	saline	1146:1151	arg1	control					1153:1159	saline control	1146:1159	saline control	1146:1159	Composite scores were significantly increased in LPS, MorMdz, and LPS/MorMdz groups compared to saline control (F3,78 = 38.1, p < 0.0001).
37302746	2	34	with	sedation	372:379	arg1	medications					392:402	these medications	386:402	these medications	386:402	However, little is known about how multidrug sedation with these medications interacts with inflammation in the developing brain, a frequent condition during childhood critical illness that has not been extensively studied.
37302746	8	35	theme	neurodevelopmental	1854:1871	arg1	effects					1873:1879	neurodevelopmental effects	1854:1879	neurodevelopmental effects	1854:1879	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	8	36	theme	critical	1698:1705	arg1	illness					1707:1713	pediatric critical illness	1688:1713	pediatric critical illness	1688:1713	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	1	37	theme	critical	204:211	arg1	illness					213:219	pediatric critical illness	194:219	pediatric critical illness	194:219	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	7	38	theme	LPS/MorMdz-treated	1605:1622	arg1	p = 0.16					1630:1637	p = 0.16	1630:1637	p = 0.16	1630:1637	Likewise, proinflammatory cytokines were increased in brains of LPS-treated pups versus Saline (p = 0.002), but not LPS/MorMdz-treated pups (p = 0.16).
37302746	7	38	theme	LPS/MorMdz-treated	1605:1622	arg1	pups					1624:1627	LPS/MorMdz-treated pups	1605:1627	LPS/MorMdz-treated pups (p = 0.16)	1605:1638	Likewise, proinflammatory cytokines were increased in brains of LPS-treated pups versus Saline (p = 0.002), but not LPS/MorMdz-treated pups (p = 0.16).
37302746	0	39	theme	delirium-like	125:137	arg1	behaviors					139:147	delirium-like behaviors	125:147	delirium-like behaviors	125:147	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	3	40	theme	mild-moderate	569:581	arg1	inflammation					583:594	mild-moderate inflammation	569:594	mild-moderate inflammation	569:594	In weanling rats, mild-moderate inflammation was induced with lipopolysaccharide (LPS) on postnatal day (P)18 and combined with 3 days repeated opioid and benzodiazepine sedation using morphine and midazolam (MorMdz) between P19-21.
37302746	7	41	theme	pups	1565:1568	arg1	brains					1543:1548	brains	1543:1548	brains of LPS-treated pups versus Saline (p = 0.002)	1543:1594	Likewise, proinflammatory cytokines were increased in brains of LPS-treated pups versus Saline (p = 0.002), but not LPS/MorMdz-treated pups (p = 0.16).
37302746	2	42	theme	developing	439:448	arg1	brain					450:454	the developing brain	435:454	the developing brain	435:454	However, little is known about how multidrug sedation with these medications interacts with inflammation in the developing brain, a frequent condition during childhood critical illness that has not been extensively studied.
37302746	6	43	theme	acidic	1331:1336	arg1	GFAP					1347:1350	GFAP	1347:1350	GFAP	1347:1350	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	43	theme	acidic	1331:1336	arg1	protein					1338:1344	glial fibrillary acidic protein	1314:1344	glial fibrillary acidic protein (GFAP)	1314:1351	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	7	44	theme	proinflammatory	1499:1513	arg1	cytokines					1515:1523	proinflammatory cytokines	1499:1523	proinflammatory cytokines	1499:1523	Likewise, proinflammatory cytokines were increased in brains of LPS-treated pups versus Saline (p = 0.002), but not LPS/MorMdz-treated pups (p = 0.16).
37302746	8	45	from	effects	1754:1760	arg1	responses					1811:1819	homeostatic neuroimmune responses	1787:1819	homeostatic neuroimmune responses	1787:1819	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	2	46	theme	critical	495:502	arg1	illness					504:510	childhood critical illness	485:510	childhood critical illness	485:510	However, little is known about how multidrug sedation with these medications interacts with inflammation in the developing brain, a frequent condition during childhood critical illness that has not been extensively studied.
37302746	1	47	theme	post-discharge	302:315	arg1	sequelae					317:324	post-discharge sequelae	302:324	post-discharge sequelae	302:324	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	4	48	theme	female	934:939	arg1	pups					945:948	male and female rat pups	925:948	male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group)	925:1003	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	5	49	theme	Composite	1050:1058	arg1	scores					1060:1065	Composite scores	1050:1065	Composite scores	1050:1065	Composite scores were significantly increased in LPS, MorMdz, and LPS/MorMdz groups compared to saline control (F3,78 = 38.1, p < 0.0001).
37302746	4	50	theme	whisker	839:845	arg1	stimulation					847:857	whisker stimulation	839:857	whisker stimulation	839:857	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	2	51	theme	childhood	485:493	arg1	illness					504:510	childhood critical illness	485:510	childhood critical illness	485:510	However, little is known about how multidrug sedation with these medications interacts with inflammation in the developing brain, a frequent condition during childhood critical illness that has not been extensively studied.
37302746	5	52	theme	LPS/MorMdz	1116:1125	arg1	groups					1127:1132	LPS, MorMdz, and LPS/MorMdz groups	1099:1132	groups	1127:1132	Composite scores were significantly increased in LPS, MorMdz, and LPS/MorMdz groups compared to saline control (F3,78 = 38.1, p < 0.0001).
37302746	0	53	theme	behaviors	139:147	arg1	induction					112:120	Potential induction	102:120	Potential induction of delirium-like behaviors	102:147	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	5	54	dep	increased	1086:1094	arg1	compared					1134:1141	compared	1134:1141	compared to saline control (F3,78 = 38.1, p < 0.0001)	1134:1186	Composite scores were significantly increased in LPS, MorMdz, and LPS/MorMdz groups compared to saline control (F3,78 = 38.1, p < 0.0001).
37302746	0	55	theme	acute	53:57	arg1	Neuroinflammation					59:75	acute Neuroinflammation	53:75	acute Neuroinflammation in the developing brain	53:99	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	0	56	from	Neuroinflammation	59:75	arg1	brain					95:99	the developing brain	80:99	the developing brain	80:99	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	6	57	theme	brain	1377:1381	arg1	homogenate					1383:1392	P22 brain homogenate	1373:1392	P22 brain homogenate	1373:1392	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	58	theme	homogenate	1383:1392	arg1	blots					1364:1368	western blots	1356:1368	western blots of P22 brain homogenate	1356:1392	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	4	59	theme	male	925:928	arg1	pups					945:948	male and female rat pups	925:948	male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group)	925:1003	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	6	60	theme	markers	1252:1258	arg1	expression					1203:1212	expression	1203:1212	expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate	1203:1392	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	1	61	from	illness	213:219	arg1	prevalent					181:189	prevalent	181:189	prevalent	181:189	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	4	62	theme	abnormal	818:825	arg1	response					827:834	abnormal response	818:834	abnormal response to whisker stimulation	818:857	Delirium-like behaviors including abnormal response to whisker stimulation, wet dog shakes, and delay in finding buried food were induced in male and female rat pups treated with LPS, MorMdz, or LPS/MorMdz (n ≥ 17/group) and were compared using a z-score composite.
37302746	3	63	theme	3 days	679:684	arg1	opioid					695:700	3 days repeated opioid	679:700	3 days repeated opioid	679:700	In weanling rats, mild-moderate inflammation was induced with lipopolysaccharide (LPS) on postnatal day (P)18 and combined with 3 days repeated opioid and benzodiazepine sedation using morphine and midazolam (MorMdz) between P19-21.
37302746	3	64	theme	postnatal	641:649	arg1	P					656:656	P	656:656	P	656:656	In weanling rats, mild-moderate inflammation was induced with lipopolysaccharide (LPS) on postnatal day (P)18 and combined with 3 days repeated opioid and benzodiazepine sedation using morphine and midazolam (MorMdz) between P19-21.
37302746	3	64	theme	postnatal	641:649	arg1	day					651:653	postnatal day (P)18	641:659	postnatal day (P)18	641:659	In weanling rats, mild-moderate inflammation was induced with lipopolysaccharide (LPS) on postnatal day (P)18 and combined with 3 days repeated opioid and benzodiazepine sedation using morphine and midazolam (MorMdz) between P19-21.
37302746	6	65	theme	glial	1314:1318	arg1	GFAP					1347:1350	GFAP	1347:1350	GFAP	1347:1350	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	6	65	theme	glial	1314:1318	arg1	protein					1338:1344	glial fibrillary acidic protein	1314:1344	glial fibrillary acidic protein (GFAP)	1314:1351	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	8	66	theme	pediatric	1688:1696	arg1	illness					1707:1713	pediatric critical illness	1688:1713	pediatric critical illness	1688:1713	These results are of potential interest during pediatric critical illness, as inflammation is ubiquitous and the effects of multidrug sedation on homeostatic neuroimmune responses need to be considered along with neurodevelopmental effects.
37302746	6	67	theme	P22	1373:1375	arg1	homogenate					1383:1392	P22 brain homogenate	1373:1392	P22 brain homogenate	1373:1392	Additionally, expression of glial-associated neuroinflammatory markers ionized calcium-binding adaptor molecule 1 (Iba1) and glial fibrillary acidic protein (GFAP) in western blots of P22 brain homogenate were significantly higher after LPS than after LPS/MorMdz (Iba1, p < 0.0001; GFAP, p < 0.001).
37302746	0	68	from	brain	95:99	arg1	context					42:48	the context	38:48	the context of acute Neuroinflammation in the developing brain	38:99	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	1	69	theme	Neurologic	150:159	arg1	morbidity					161:169	Neurologic morbidity	150:169	Neurologic morbidity	150:169	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	1	70	theme	benzodiazepines	237:251	arg1	use					230:232	the use	226:232	the use of benzodiazepines and/or opioids	226:266	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	1	70	theme	benzodiazepines	237:251	arg1	factors					277:283	risk factors	272:283	risk factors for delirium and post-discharge sequelae	272:324	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	0	71	theme	Neuroinflammation	59:75	arg1	context					42:48	the context	38:48	the context of acute Neuroinflammation in the developing brain	38:99	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	0	72	dep	Understanding	0:12	arg1	induction					112:120	Potential induction	102:120	Potential induction of delirium-like behaviors	102:147	Understanding sedative effects within the context of acute Neuroinflammation in the developing brain: Potential induction of delirium-like behaviors.
37302746	1	73	from	prevalent	181:189	arg1	illness					213:219	pediatric critical illness	194:219	pediatric critical illness	194:219	Neurologic morbidity is highly prevalent in pediatric critical illness, and the use of benzodiazepines and/or opioids are risk factors for delirium and post-discharge sequelae.
37302746	2	74	theme	frequent	459:466	arg1	condition					468:476	a frequent condition	457:476	a frequent condition during childhood critical illness that has not been extensively studied	457:548	However, little is known about how multidrug sedation with these medications interacts with inflammation in the developing brain, a frequent condition during childhood critical illness that has not been extensively studied.
37302746	7	75	theme	LPS-treated	1553:1563	arg1	pups					1565:1568	LPS-treated pups	1553:1568	LPS-treated pups	1553:1568	Likewise, proinflammatory cytokines were increased in brains of LPS-treated pups versus Saline (p = 0.002), but not LPS/MorMdz-treated pups (p = 0.16).
35037700	6	0	dep	generated	846:854	arg1	Rosetta					838:844	Rosetta	838:844	Rosetta	838:844	The structure was solved by molecular replacement using a Rosetta generated structure as a search model.
35037700	11	1	theme	antibody	1567:1574	arg1	recognition					1576:1586	antibody recognition	1567:1586	antibody recognition	1567:1586	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	1	2	gly	glycoprotein	131:142	arg1	glycoprotein					131:142	a membrane bound glycoprotein	114:142	a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer	114:218	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	1	2	gly	glycoprotein	131:142	arg1	MUC16					105:109	MUC16	105:109	MUC16	105:109	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	10	3	theme	SEA	1325:1327	arg1	domain					1329:1334	the glycosylated SEA domain	1308:1334	the glycosylated SEA domain	1308:1334	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	8	4	theme	disulfide	1108:1116	arg1	bond					1118:1121	a conserved disulfide bond	1096:1121	a conserved disulfide bond	1096:1121	The structure revealed a canonical ferredoxin-like fold, and contained a conserved disulfide bond.
35037700	11	5	theme	CA125	1595:1599	arg1	epitope					1601:1607	the CA125 epitope	1591:1607	the CA125 epitope	1591:1607	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	6	6	theme	molecular	808:816	arg1	replacement					818:828	molecular replacement	808:828	molecular replacement using a Rosetta generated structure as a search model	808:882	The structure was solved by molecular replacement using a Rosetta generated structure as a search model.
35037700	4	7	theme	SEA	636:638	arg1	domains					680:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	9	8	theme	solvent	1149:1155	arg1	accessibility					1157:1169	the relative solvent accessibility	1136:1169	the relative solvent accessibility of side chains within the SEA domain	1136:1206	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	2	9	theme	resulting	264:272	arg1	CA125					294:298	the resulting cancer antigen 125 (CA125)	260:299	the resulting cancer antigen 125 (CA125)	260:299	The protein is shed into the serum and the resulting cancer antigen 125 (CA125) can be detected by immunoassays.
35037700	5	10	dep	purification	708:719	arg1	the					704:706	the	704:706	the	704:706	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	0	11	theme	tumor	91:95	arg1	marker					97:102	the CA125 tumor marker	81:102	the CA125 tumor marker	81:102	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	5	12	theme	domain	772:777	arg1	structure					741:749	the first X-ray structure	725:749	the first X-ray structure	725:749	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	5	12	theme	domain	772:777	arg1	purification					708:719	purification	708:719	purification	708:719	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	4	13	theme	sequence	600:607	arg1	segments					567:574	repeating segments	557:574	repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains	557:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	9	14	theme	chains	1179:1184	arg1	accessibility					1157:1169	the relative solvent accessibility	1136:1169	the relative solvent accessibility of side chains within the SEA domain	1136:1206	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	11	15	theme	recognition	1576:1586	arg1	role					1537:1540	the functional role	1522:1540	the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope	1522:1607	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	7	16	theme	therapeutic	935:945	arg1	antibodies					947:956	three different MUC16 therapeutic antibodies	913:956	three different MUC16 therapeutic antibodies	913:956	The SEA domain reacted with three different MUC16 therapeutic antibodies, confirming that the CA125 epitope is localized to the SEA domain.
35037700	7	17	theme	different	919:927	arg1	antibodies					947:956	three different MUC16 therapeutic antibodies	913:956	three different MUC16 therapeutic antibodies	913:956	The SEA domain reacted with three different MUC16 therapeutic antibodies, confirming that the CA125 epitope is localized to the SEA domain.
35037700	4	18	theme	Sperm	641:645	arg1	protein					647:653	Sperm protein	641:653	Sperm protein	641:653	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	1	19	theme	bound	125:129	arg1	glycoprotein					131:142	a membrane bound glycoprotein	114:142	a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer	114:218	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	1	19	theme	bound	125:129	arg1	MUC16					105:109	MUC16	105:109	MUC16	105:109	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	0	20	theme	domain	39:44	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of a human MUC16 SEA domain	0:44	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	3	21	theme	ovarian	375:381	arg1	progression					400:410	ovarian cancer treatment progression	375:410	ovarian cancer treatment progression	375:410	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	9	22	theme	glycosylation	1258:1270	arg1	sites					1272:1276	N-linked and O-linked glycosylation sites	1236:1276	N-linked and O-linked glycosylation sites within the domain	1236:1294	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	10	23	theme	binding	1400:1406	arg1	antibodies					1432:1441	CA125 specific antibodies	1417:1441	CA125 specific antibodies	1417:1441	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	10	23	theme	binding	1400:1406	arg1	sites					1408:1412	the binding sites	1396:1412	the binding sites of CA125 specific antibodies	1396:1441	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	0	24	theme	Crystal	0:6	arg1	structure					8:16	Crystal structure	0:16	Crystal structure of a human MUC16 SEA domain	0:44	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	4	25	theme	extracellular	492:504	arg1	protein					534:540	the protein	530:540	the protein	530:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	4	25	theme	extracellular	492:504	arg1	domain					520:525	The extracellular tandem repeat domain	488:525	The extracellular tandem repeat domain of the protein	488:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	5	26	theme	MUC16	762:766	arg1	domain					772:777	a human MUC16 SEA domain	754:777	a human MUC16 SEA domain	754:777	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	8	27	contain	contained	1086:1094	arg2	bond					1118:1121	a conserved disulfide bond	1096:1121	a conserved disulfide bond	1096:1121	The structure revealed a canonical ferredoxin-like fold, and contained a conserved disulfide bond.
35037700	8	27	contain	contained	1086:1094	arg1	structure					1029:1037	The structure	1025:1037	The structure	1025:1037	The structure revealed a canonical ferredoxin-like fold, and contained a conserved disulfide bond.
35037700	4	28	theme	repeat	513:518	arg1	protein					534:540	the protein	530:540	the protein	530:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	4	28	theme	repeat	513:518	arg1	domain					520:525	The extracellular tandem repeat domain	488:525	The extracellular tandem repeat domain of the protein	488:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	10	29	theme	specific	1423:1430	arg1	antibodies					1432:1441	CA125 specific antibodies	1417:1441	CA125 specific antibodies	1417:1441	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	3	30	theme	treatment	390:398	arg1	progression					400:410	ovarian cancer treatment progression	375:410	ovarian cancer treatment progression	375:410	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	0	31	theme	human	23:27	arg1	domain					39:44	a human MUC16 SEA domain	21:44	a human MUC16 SEA domain	21:44	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	5	32	theme	first	729:733	arg1	structure					741:749	the first X-ray structure	725:749	the first X-ray structure	725:749	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	10	33	gly	glycosylated	1312:1323	arg1	domain					1329:1334	the glycosylated SEA domain	1308:1334	the glycosylated SEA domain	1308:1334	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	0	34	theme	SEA	35:37	arg1	domain					39:44	a human MUC16 SEA domain	21:44	a human MUC16 SEA domain	21:44	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	11	35	theme	functional	1526:1535	arg1	role					1537:1540	the functional role	1522:1540	the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope	1522:1607	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	1	36	theme	pancreatic	190:199	arg1	cancer					213:218	pancreatic and ovarian cancer	190:218	cancer	213:218	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	1	37	dep	progression	160:170	arg1	the					156:158	the	156:158	the	156:158	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	1	38	theme	ovarian	205:211	arg1	cancer					213:218	pancreatic and ovarian cancer	190:218	cancer	213:218	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	3	39	theme	CA125	338:342	arg1	epitope					344:350	The CA125 epitope	334:350	The CA125 epitope	334:350	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	3	39	theme	CA125	338:342	arg1	target					444:449	a potential target	432:449	a potential target for antibody mediated immunotherapy	432:485	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	4	40	theme	repeating	557:565	arg1	segments					567:574	repeating segments	557:574	repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains	557:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	11	41	theme	SEA	1551:1553	arg1	domains					1555:1561	MUC16 SEA domains	1545:1561	MUC16 SEA domains	1545:1561	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	3	42	theme	potential	434:442	arg1	target					444:449	a potential target	432:449	a potential target for antibody mediated immunotherapy	432:485	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	3	42	theme	potential	434:442	arg1	epitope					344:350	The CA125 epitope	334:350	The CA125 epitope	334:350	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	6	43	theme	generated	846:854	arg1	model					878:882	a search model	869:882	a search model	869:882	The structure was solved by molecular replacement using a Rosetta generated structure as a search model.
35037700	6	43	theme	generated	846:854	arg1	structure					856:864	a Rosetta generated structure	836:864	a Rosetta generated structure	836:864	The structure was solved by molecular replacement using a Rosetta generated structure as a search model.
35037700	7	44	theme	SEA	1013:1015	arg1	domain					1017:1022	the SEA domain	1009:1022	the SEA domain	1009:1022	The SEA domain reacted with three different MUC16 therapeutic antibodies, confirming that the CA125 epitope is localized to the SEA domain.
35037700	10	45	theme	accessible	1355:1364	arg1	faces					1366:1370	two major accessible faces	1345:1370	two major accessible faces	1345:1370	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	10	46	theme	domain	1329:1334	arg1	model					1299:1303	A model	1297:1303	A model of the glycosylated SEA domain	1297:1334	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	4	47	theme	protein	534:540	arg1	protein					534:540	the protein	530:540	the protein	530:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	4	47	theme	protein	534:540	arg1	domain					520:525	The extracellular tandem repeat domain	488:525	The extracellular tandem repeat domain of the protein	488:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	10	48	theme	glycosylated	1312:1323	arg1	domain					1329:1334	the glycosylated SEA domain	1308:1334	the glycosylated SEA domain	1308:1334	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	3	49	theme	mediated	464:471	arg1	immunotherapy					473:485	antibody mediated immunotherapy	455:485	antibody mediated immunotherapy	455:485	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	8	50	theme	conserved	1098:1106	arg1	bond					1118:1121	a conserved disulfide bond	1096:1121	a conserved disulfide bond	1096:1121	The structure revealed a canonical ferredoxin-like fold, and contained a conserved disulfide bond.
35037700	11	51	theme	epitope	1601:1607	arg1	role					1537:1540	the functional role	1522:1540	the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope	1522:1607	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	4	52	gly	glycosylated	587:598	arg1	sequence					600:607	heavily glycosylated sequence	579:607	heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains	579:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	9	53	theme	relative	1140:1147	arg1	accessibility					1157:1169	the relative solvent accessibility	1136:1169	the relative solvent accessibility of side chains within the SEA domain	1136:1206	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	2	54	theme	cancer	274:279	arg1	CA125					294:298	the resulting cancer antigen 125 (CA125)	260:299	the resulting cancer antigen 125 (CA125)	260:299	The protein is shed into the serum and the resulting cancer antigen 125 (CA125) can be detected by immunoassays.
35037700	4	55	theme	homologous	625:634	arg1	domains					680:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	10	56	theme	major	1349:1353	arg1	faces					1366:1370	two major accessible faces	1345:1370	two major accessible faces	1345:1370	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	0	57	theme	marker	97:102	arg1	nature					71:76	the nature	67:76	the nature of the CA125 tumor marker	67:102	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	9	58	theme	accessibility	1157:1169	arg1	Analysis					1124:1131	Analysis	1124:1131	Analysis of the relative solvent accessibility of side chains within the SEA domain	1124:1206	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	5	59	theme	SEA	768:770	arg1	domain					772:777	a human MUC16 SEA domain	754:777	a human MUC16 SEA domain	754:777	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	4	60	theme	protein	647:653	arg1	domains					680:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	9	61	theme	side	1174:1177	arg1	chains					1179:1184	side chains	1174:1184	side chains	1174:1184	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	11	62	theme	future	1496:1501	arg1	work					1503:1506	future work	1496:1506	future work	1496:1506	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	4	63	theme	glycosylated	587:598	arg1	sequence					600:607	heavily glycosylated sequence	579:607	heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains	579:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	7	64	theme	CA125	979:983	arg1	epitope					985:991	the CA125 epitope	975:991	the CA125 epitope	975:991	The SEA domain reacted with three different MUC16 therapeutic antibodies, confirming that the CA125 epitope is localized to the SEA domain.
35037700	3	65	used	used	355:358	arg2	epitope					344:350	The CA125 epitope	334:350	The CA125 epitope	334:350	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	3	65	used	used	355:358	arg2	target					444:449	a potential target	432:449	a potential target for antibody mediated immunotherapy	432:485	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	6	66	theme	search	871:876	arg1	model					878:882	a search model	869:882	a search model	869:882	The structure was solved by molecular replacement using a Rosetta generated structure as a search model.
35037700	6	66	theme	search	871:876	arg1	structure					856:864	a Rosetta generated structure	836:864	a Rosetta generated structure	836:864	The structure was solved by molecular replacement using a Rosetta generated structure as a search model.
35037700	4	67	theme	Enterokinase	656:667	arg1	domains					680:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	9	68	theme	SEA	1197:1199	arg1	domain					1201:1206	the SEA domain	1193:1206	the SEA domain	1193:1206	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	4	69	theme	Agrin	673:677	arg1	domains					680:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	homologous SEA (Sperm protein, Enterokinase and Agrin) domains	625:686	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	7	70	theme	MUC16	929:933	arg1	antibodies					947:956	three different MUC16 therapeutic antibodies	913:956	three different MUC16 therapeutic antibodies	913:956	The SEA domain reacted with three different MUC16 therapeutic antibodies, confirming that the CA125 epitope is localized to the SEA domain.
35037700	1	71	theme	membrane	116:123	arg1	glycoprotein					131:142	a membrane bound glycoprotein	114:142	a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer	114:218	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	1	71	theme	membrane	116:123	arg1	MUC16					105:109	MUC16	105:109	MUC16	105:109	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	9	72	gly	glycosylation	1258:1270	arg2	sites					1272:1276	N-linked and O-linked glycosylation sites	1236:1276	N-linked and O-linked glycosylation sites within the domain	1236:1294	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	9	73	theme	N-linked	1236:1243	arg1	sites					1272:1276	N-linked and O-linked glycosylation sites	1236:1276	N-linked and O-linked glycosylation sites within the domain	1236:1294	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	0	74	theme	CA125	85:89	arg1	marker					97:102	the CA125 tumor marker	81:102	the CA125 tumor marker	81:102	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	9	75	theme	O-linked	1249:1256	arg1	sites					1272:1276	N-linked and O-linked glycosylation sites	1236:1276	N-linked and O-linked glycosylation sites within the domain	1236:1294	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	7	76	theme	SEA	889:891	arg1	domain					893:898	The SEA domain	885:898	The SEA domain	885:898	The SEA domain reacted with three different MUC16 therapeutic antibodies, confirming that the CA125 epitope is localized to the SEA domain.
35037700	3	77	theme	cancer	383:388	arg1	progression					400:410	ovarian cancer treatment progression	375:410	ovarian cancer treatment progression	375:410	The CA125 epitope is used for monitoring ovarian cancer treatment progression, and has emerged as a potential target for antibody mediated immunotherapy.
35037700	9	78	theme	sites	1272:1276	arg1	assignment					1222:1231	the assignment	1218:1231	the assignment of N-linked and O-linked glycosylation sites within the domain	1218:1294	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	9	79	link	O-linked	1249:1256	arg1	sites					1272:1276	N-linked and O-linked glycosylation sites	1236:1276	N-linked and O-linked glycosylation sites within the domain	1236:1294	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	4	80	theme	tandem	506:511	arg1	protein					534:540	the protein	530:540	the protein	530:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	4	80	theme	tandem	506:511	arg1	domain					520:525	The extracellular tandem repeat domain	488:525	The extracellular tandem repeat domain of the protein	488:540	The extracellular tandem repeat domain of the protein is composed of repeating segments of heavily glycosylated sequence intermixed with homologous SEA (Sperm protein, Enterokinase and Agrin) domains.
35037700	5	81	theme	human	756:760	arg1	domain					772:777	a human MUC16 SEA domain	754:777	a human MUC16 SEA domain	754:777	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	9	82	link	N-linked	1236:1243	arg1	sites					1272:1276	N-linked and O-linked glycosylation sites	1236:1276	N-linked and O-linked glycosylation sites within the domain	1236:1294	Analysis of the relative solvent accessibility of side chains within the SEA domain clarified the assignment of N-linked and O-linked glycosylation sites within the domain.
35037700	10	83	theme	antibodies	1432:1441	arg1	antibodies					1432:1441	CA125 specific antibodies	1417:1441	CA125 specific antibodies	1417:1441	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	10	83	theme	antibodies	1432:1441	arg1	sites					1408:1412	the binding sites	1396:1412	the binding sites of CA125 specific antibodies	1396:1441	A model of the glycosylated SEA domain revealed two major accessible faces, which likely represent the binding sites of CA125 specific antibodies.
35037700	0	84	theme	MUC16	29:33	arg1	domain					39:44	a human MUC16 SEA domain	21:44	a human MUC16 SEA domain	21:44	Crystal structure of a human MUC16 SEA domain reveals insight into the nature of the CA125 tumor marker.
35037700	5	85	theme	X-ray	735:739	arg1	structure					741:749	the first X-ray structure	725:749	the first X-ray structure	725:749	Here we report the purification and the first X-ray structure of a human MUC16 SEA domain.
35037700	8	86	theme	ferredoxin-like	1060:1074	arg1	fold					1076:1079	a canonical ferredoxin-like fold	1048:1079	a canonical ferredoxin-like fold	1048:1079	The structure revealed a canonical ferredoxin-like fold, and contained a conserved disulfide bond.
35037700	8	87	theme	canonical	1050:1058	arg1	fold					1076:1079	a canonical ferredoxin-like fold	1048:1079	a canonical ferredoxin-like fold	1048:1079	The structure revealed a canonical ferredoxin-like fold, and contained a conserved disulfide bond.
35037700	11	88	theme	domains	1555:1561	arg1	role					1537:1540	the functional role	1522:1540	the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope	1522:1607	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	2	89	theme	antigen	281:287	arg1	CA125					294:298	the resulting cancer antigen 125 (CA125)	260:299	the resulting cancer antigen 125 (CA125)	260:299	The protein is shed into the serum and the resulting cancer antigen 125 (CA125) can be detected by immunoassays.
35037700	11	90	theme	MUC16	1545:1549	arg1	domains					1555:1561	MUC16 SEA domains	1545:1561	MUC16 SEA domains	1545:1561	The results presented here will serve to accelerate future work to understand the functional role of MUC16 SEA domains and antibody recognition of the CA125 epitope.
35037700	1	91	theme	cancer	213:218	arg1	metastasis					176:185	metastasis	176:185	metastasis	176:185	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
35037700	1	91	theme	cancer	213:218	arg1	progression					160:170	progression	160:170	progression	160:170	MUC16 is a membrane bound glycoprotein involved in the progression and metastasis of pancreatic and ovarian cancer.
36401221	2	0	theme	intestinal	338:347	arg1	functions					356:364	intestinal immune functions	338:364	intestinal immune functions	338:364	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	2	1	from	microbiome	323:332	arg1	models					400:405	healthy and ulcerative colitis models	369:405	healthy and ulcerative colitis models	369:405	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	4	2	theme	colon	623:627	arg1	tissues					629:635	colon tissues	623:635	colon tissues	623:635	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	13	3	theme	gut	1870:1872	arg1	microbiota					1874:1883	gut microbiota	1870:1883	gut microbiota	1870:1883	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	5	4	theme	ulcerative	772:781	arg1	model					796:800	ulcerative colitis mice model	772:800	ulcerative colitis mice model	772:800	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	2	5	theme	triclosan	297:305	arg1	exposure					307:314	triclosan exposure	297:314	triclosan exposure	297:314	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	5	6	theme	4th	827:829	arg1	week					831:834	the 4th week	823:834	the 4th week	823:834	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	12	7	from	colitis	1819:1825	arg1	mice					1830:1833	mice	1830:1833	mice	1830:1833	CONCLUSION Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	2	8	from	functions	356:364	arg1	models					400:405	healthy and ulcerative colitis models	369:405	healthy and ulcerative colitis models	369:405	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	11	9	theme	butyrate-producing	1693:1710	arg1	bacteria					1712:1719	Bacteroides, and less butyrate-producing bacteria	1671:1719	bacteria	1712:1719	Fecal samples showed higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria.
36401221	0	10	from	colitis	86:92	arg1	mice					97:100	mice	97:100	mice	97:100	Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	2	11	theme	healthy	369:375	arg1	models					400:405	healthy and ulcerative colitis models	369:405	healthy and ulcerative colitis models	369:405	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	13	12	theme	butyrate-producing	2038:2055	arg1	probiotics					2026:2035	protective probiotics	2015:2035	protective probiotics	2015:2035	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	13	12	theme	butyrate-producing	2038:2055	arg1	bacteria					2057:2064	butyrate-producing bacteria	2038:2064	butyrate-producing bacteria	2038:2064	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	4	13	theme	fecal	686:690	arg1	samples					692:698	fecal samples	686:698	fecal samples	686:698	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	11	14	theme	bacteria	1658:1665	arg1	enrichment					1627:1636	higher enrichment	1620:1636	higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria	1620:1719	Fecal samples showed higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria.
36401221	2	15	theme	animal	247:252	arg1	experiment					254:263	This animal experiment	242:263	This animal experiment	242:263	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	5	16	theme	mice	791:794	arg1	model					796:800	ulcerative colitis mice model	772:800	ulcerative colitis mice model	772:800	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	1	17	theme	personal	151:158	arg1	products					165:172	personal care products	151:172	personal care products	151:172	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	8	18	theme	DSS-induced	1209:1219	arg1	mice					1221:1224	DSS-induced mice	1209:1224	DSS-induced mice	1209:1224	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	7	19	theme	Toll-like	1117:1125	arg1	receptor					1127:1134	Toll-like receptor 4	1117:1136	Toll-like receptor 4 (TLR4)	1117:1143	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	7	19	theme	Toll-like	1117:1125	arg1	TLR4					1139:1142	TLR4	1139:1142	TLR4	1139:1142	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	13	20	theme	probiotics	2026:2035	arg1	reduction					2002:2010	the reduction	1998:2010	the reduction of protective probiotics, butyrate-producing bacteria	1998:2064	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	13	20	theme	probiotics	2026:2035	arg1	increase					1911:1918	the increase	1907:1918	the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides,	1907:1992	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	4	21	theme	rRNA	724:727	arg1	sequencing					729:738	16 S rRNA sequencing	719:738	16 S rRNA sequencing of gut microbiota	719:756	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	2	22	dep	microbiome	323:332	arg1	the					319:321	the	319:321	the	319:321	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	11	23	theme	Bacteroides	1671:1681	arg1	bacteria					1712:1719	Bacteroides, and less butyrate-producing bacteria	1671:1719	bacteria	1712:1719	Fecal samples showed higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria.
36401221	7	24	theme	p-NF-κb	1105:1111	arg1	levels					1095:1100	the levels	1091:1100	the levels of p-NF-κb and Toll-like receptor 4 (TLR4)	1091:1143	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	13	25	from	changes	1840:1846	arg1	composition					1855:1865	the composition	1851:1865	the composition of gut microbiota	1851:1883	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	8	26	theme	inflammatory	1390:1401	arg1	cells					1403:1407	inflammatory cells	1390:1407	inflammatory cells	1390:1407	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	2	27	theme	colitis	392:398	arg1	models					400:405	healthy and ulcerative colitis models	369:405	healthy and ulcerative colitis models	369:405	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	0	28	theme	gut	42:44	arg1	microbiota					46:55	gut microbiota	42:55	gut microbiota	42:55	Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	5	29	theme	%	910:910	arg1	DSS					935:937	2% Dextran sulfate sodium(DSS)	909:938	2% Dextran sulfate sodium(DSS)	909:938	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	0	30	theme	experimental	73:84	arg1	colitis					86:92	experimental colitis	73:92	experimental colitis in mice	73:100	Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	8	31	theme	inflammatory	1249:1260	arg1	cytokines					1262:1270	inflammatory cytokines	1249:1270	inflammatory cytokines	1249:1270	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	0	32	theme	Triclosan	0:8	arg1	exposure					10:17	Triclosan exposure	0:17	Triclosan exposure	0:17	Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	9	33	theme	intraepithelial	1450:1464	arg1	neoplasia					1466:1474	Low-grade intraepithelial neoplasia	1440:1474	Low-grade intraepithelial neoplasia	1440:1474	Low-grade intraepithelial neoplasia appeared.
36401221	7	34	theme	common	1070:1075	arg1	mice					1077:1080	common mice	1070:1080	common mice	1070:1080	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	3	35	theme	METHODS	408:414	arg1	mice					423:426	METHODS Balb/c mice	408:426	METHODS Balb/c mice	408:426	METHODS Balb/c mice were maintained on an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks.
36401221	8	36	theme	cells	1433:1437	arg1	infiltration					1374:1385	infiltration	1374:1385	infiltration of inflammatory cells	1374:1407	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	8	36	theme	cells	1433:1437	arg1	degree					1325:1330	the degree	1321:1330	the degree of damage to intestinal mucosa and crypt	1321:1371	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	8	36	theme	cells	1433:1437	arg1	atypia					1413:1418	atypia	1413:1418	atypia of glandular cells	1413:1437	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	11	37	theme	Fecal	1599:1603	arg1	samples					1605:1611	Fecal samples	1599:1611	Fecal samples	1599:1611	Fecal samples showed higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria.
36401221	7	38	theme	RESULTS	1040:1046	arg1	exposure					1058:1065	RESULTS Triclosan exposure	1040:1065	RESULTS Triclosan exposure to common mice	1040:1080	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	6	39	theme	gut	1024:1026	arg1	analysis					1000:1007	analysis	1000:1007	analysis of colitis and gut microbiota	1000:1037	Then mice were sacrificed for analysis of colitis and gut microbiota.
36401221	4	40	theme	microbiota	747:756	arg1	sequencing					729:738	16 S rRNA sequencing	719:738	16 S rRNA sequencing of gut microbiota	719:756	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	10	41	theme	microbiota	1587:1596	arg1	composition					1568:1578	composition	1568:1578	composition	1568:1578	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
36401221	10	41	theme	microbiota	1587:1596	arg1	diversity					1554:1562	diversity	1554:1562	diversity	1554:1562	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
36401221	12	42	theme	microbiota	1779:1788	arg1	disturbance					1760:1770	disturbance	1760:1770	disturbance of gut microbiota	1760:1788	CONCLUSION Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	5	43	theme	sulfate	920:926	arg1	DSS					935:937	2% Dextran sulfate sodium(DSS)	909:938	2% Dextran sulfate sodium(DSS)	909:938	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	6	44	theme	colitis	1012:1018	arg1	analysis					1000:1007	analysis	1000:1007	analysis of colitis and gut microbiota	1000:1037	Then mice were sacrificed for analysis of colitis and gut microbiota.
36401221	13	45	theme	bacteria	1931:1938	arg1	reduction					2002:2010	the reduction	1998:2010	the reduction of protective probiotics, butyrate-producing bacteria	1998:2064	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	13	45	theme	bacteria	1931:1938	arg1	increase					1911:1918	the increase	1907:1918	the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides,	1907:1992	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	1	46	theme	digestive	225:233	arg1	tract					235:239	the digestive tract	221:239	the digestive tract	221:239	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	6	47	dep	colitis	1012:1018	arg1	microbiota					1028:1037	microbiota	1028:1037	microbiota	1028:1037	Then mice were sacrificed for analysis of colitis and gut microbiota.
36401221	12	48	theme	CONCLUSION	1722:1731	arg1	exposure					1743:1750	CONCLUSION Triclosan exposure	1722:1750	CONCLUSION Triclosan exposure	1722:1750	CONCLUSION Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	2	49	theme	immune	349:354	arg1	functions					356:364	intestinal immune functions	338:364	intestinal immune functions	338:364	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	0	50	theme	microbiota	46:55	arg1	disturbance					27:37	disturbance	27:37	disturbance of gut microbiota	27:55	Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	10	51	theme	common	1494:1499	arg1	mice					1517:1520	common and DSS-induced mice	1494:1520	common and DSS-induced mice	1494:1520	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
36401221	13	52	theme	microbiota	1874:1883	arg1	composition					1855:1865	the composition	1851:1865	the composition of gut microbiota	1851:1883	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	2	53	theme	exposure	307:314	arg1	effects					286:292	the effects	282:292	the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models	282:405	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	11	54	theme	bacteria	1712:1719	arg1	enrichment					1627:1636	higher enrichment	1620:1636	higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria	1620:1719	Fecal samples showed higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria.
36401221	1	55	theme	human	202:206	arg1	body					208:211	the human body	198:211	the human body	198:211	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	8	56	theme	damage	1335:1340	arg1	infiltration					1374:1385	infiltration	1374:1385	infiltration of inflammatory cells	1374:1407	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	8	56	theme	damage	1335:1340	arg1	degree					1325:1330	the degree	1321:1330	the degree of damage to intestinal mucosa and crypt	1321:1371	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	8	56	theme	damage	1335:1340	arg1	atypia					1413:1418	atypia	1413:1418	atypia of glandular cells	1413:1437	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	4	57	theme	blood	605:609	arg1	samples					611:617	blood samples	605:617	blood samples	605:617	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	10	58	theme	DSS-induced	1505:1515	arg1	mice					1517:1520	common and DSS-induced mice	1494:1520	common and DSS-induced mice	1494:1520	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
36401221	8	59	theme	intestinal	1345:1354	arg1	mucosa					1356:1361	intestinal mucosa	1345:1361	intestinal mucosa	1345:1361	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	4	60	theme	inflammation	668:679	arg1	analysis					656:663	analysis	656:663	analysis of inflammation	656:679	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	7	61	from	Occludin	1164:1171	arg1	colon					1180:1184	the colon	1176:1184	the colon	1176:1184	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	1	62	theme	antimicrobial	128:140	arg1	agent					142:146	an antimicrobial agent	125:146	an antimicrobial agent in personal care products	125:172	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	1	62	theme	antimicrobial	128:140	arg1	Triclosan					114:122	BACKGROUND Triclosan	103:122	BACKGROUND Triclosan	103:122	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	3	63	theme	80ppm	474:478	arg1	triclosan					480:488	80ppm triclosan	474:488	80ppm triclosan dissolved in polyethylene	474:514	METHODS Balb/c mice were maintained on an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks.
36401221	7	64	theme	receptor	1127:1134	arg1	levels					1095:1100	the levels	1091:1100	the levels of p-NF-κb and Toll-like receptor 4 (TLR4)	1091:1143	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	5	65	theme	week	831:834	arg1	beginning					810:818	the beginning	806:818	the beginning of the 4th week	806:834	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	8	66	theme	Triclosan	1187:1195	arg1	exposure					1197:1204	Triclosan exposure	1187:1204	Triclosan exposure to DSS-induced mice	1187:1224	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	11	67	theme	sulfate-reducing	1641:1656	arg1	bacteria					1658:1665	sulfate-reducing bacteria	1641:1665	sulfate-reducing bacteria	1641:1665	Fecal samples showed higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria.
36401221	13	68	theme	protective	2015:2024	arg1	probiotics					2026:2035	protective probiotics	2015:2035	protective probiotics	2015:2035	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	13	68	theme	protective	2015:2024	arg1	bacteria					2057:2064	butyrate-producing bacteria	2038:2064	butyrate-producing bacteria	2038:2064	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	1	69	theme	care	160:163	arg1	products					165:172	personal care products	151:172	personal care products	151:172	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	2	70	from	effects	286:292	arg1	microbiome					323:332	microbiome	323:332	microbiome	323:332	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	2	70	from	effects	286:292	arg1	functions					356:364	intestinal immune functions	338:364	intestinal immune functions	338:364	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	8	71	theme	Occludin	1295:1302	arg1	levels					1285:1290	the levels	1281:1290	the levels of Occludin	1281:1302	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	8	72	theme	cells	1403:1407	arg1	infiltration					1374:1385	infiltration	1374:1385	infiltration of inflammatory cells	1374:1407	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	8	72	theme	cells	1403:1407	arg1	degree					1325:1330	the degree	1321:1330	the degree of damage to intestinal mucosa and crypt	1321:1371	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	8	72	theme	cells	1403:1407	arg1	atypia					1413:1418	atypia	1413:1418	atypia of glandular cells	1413:1437	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	4	73	theme	16 S	719:722	arg1	sequencing					729:738	16 S rRNA sequencing	719:738	16 S rRNA sequencing of gut microbiota	719:756	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	5	74	theme	2	909:909	arg1	%					910:910	%	910:910	%	910:910	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	2	75	theme	ulcerative	381:390	arg1	colitis					392:398	ulcerative colitis	381:398	ulcerative colitis	381:398	This animal experiment aimed to clarify the effects of triclosan exposure on the microbiome and intestinal immune functions in healthy and ulcerative colitis models.
36401221	1	76	from	agent	142:146	arg1	products					165:172	personal care products	151:172	personal care products	151:172	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	9	77	theme	Low-grade	1440:1448	arg1	neoplasia					1466:1474	Low-grade intraepithelial neoplasia	1440:1474	Low-grade intraepithelial neoplasia	1440:1474	Low-grade intraepithelial neoplasia appeared.
36401221	8	78	theme	glandular	1423:1431	arg1	cells					1433:1437	glandular cells	1423:1437	glandular cells	1423:1437	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	10	79	theme	triclosan	1523:1531	arg1	exposure					1533:1540	triclosan exposure	1523:1540	triclosan exposure	1523:1540	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
36401221	5	80	theme	colitis	783:789	arg1	model					796:800	ulcerative colitis mice model	772:800	ulcerative colitis mice model	772:800	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	12	81	theme	experimental	1806:1817	arg1	colitis					1819:1825	experimental colitis	1806:1825	experimental colitis in mice	1806:1833	CONCLUSION Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	1	82	theme	BACKGROUND	103:112	arg1	agent					142:146	an antimicrobial agent	125:146	an antimicrobial agent in personal care products	125:172	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	1	82	theme	BACKGROUND	103:112	arg1	Triclosan					114:122	BACKGROUND Triclosan	103:122	BACKGROUND Triclosan	103:122	BACKGROUND Triclosan, an antimicrobial agent in personal care products, could be absorbed into the human body through the digestive tract.
36401221	8	83	theme	cytokines	1262:1270	arg1	level					1240:1244	the level	1236:1244	the level of inflammatory cytokines	1236:1270	Triclosan exposure to DSS-induced mice increased the level of inflammatory cytokines, reduced the levels of Occludin, and exacerbated the degree of damage to intestinal mucosa and crypt, infiltration of inflammatory cells and atypia of glandular cells.
36401221	7	84	theme	Triclosan	1048:1056	arg1	exposure					1058:1065	RESULTS Triclosan exposure	1040:1065	RESULTS Triclosan exposure to common mice	1040:1080	RESULTS Triclosan exposure to common mice enhanced the levels of p-NF-κb and Toll-like receptor 4 (TLR4), and decreased the Occludin in the colon.
36401221	3	85	theme	Balb/c	416:421	arg1	mice					423:426	METHODS Balb/c mice	408:426	METHODS Balb/c mice	408:426	METHODS Balb/c mice were maintained on an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks.
36401221	10	86	dep	diversity	1554:1562	arg1	the					1550:1552	the	1550:1552	the	1550:1552	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
36401221	13	87	theme	sulfate-reducing	1951:1966	arg1	bacteria					1968:1975	sulfate-reducing bacteria	1951:1975	sulfate-reducing bacteria	1951:1975	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	3	88	theme	AIN-93G	450:456	arg1	diet					458:461	an AIN-93G diet	447:461	an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks	447:564	METHODS Balb/c mice were maintained on an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks.
36401221	12	89	theme	gut	1775:1777	arg1	microbiota					1779:1788	gut microbiota	1775:1788	gut microbiota	1775:1788	CONCLUSION Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	12	90	theme	Triclosan	1733:1741	arg1	exposure					1743:1750	CONCLUSION Triclosan exposure	1722:1750	CONCLUSION Triclosan exposure	1722:1750	CONCLUSION Triclosan exposure induced disturbance of gut microbiota and exaggerated experimental colitis in mice.
36401221	11	91	theme	higher	1620:1625	arg1	enrichment					1627:1636	higher enrichment	1620:1636	higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria	1620:1719	Fecal samples showed higher enrichment of sulfate-reducing bacteria and Bacteroides, and less butyrate-producing bacteria.
36401221	3	92	contain	containing	463:472	arg1	diet					458:461	an AIN-93G diet	447:461	an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks	447:564	METHODS Balb/c mice were maintained on an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks.
36401221	3	92	contain	containing	463:472	arg2	triclosan					480:488	80ppm triclosan	474:488	80ppm triclosan dissolved in polyethylene	474:514	METHODS Balb/c mice were maintained on an AIN-93G diet containing 80ppm triclosan dissolved in polyethylene as vehicle or vehicle alone for 1 week or 4 weeks.
36401221	5	93	theme	Dextran	912:918	arg1	DSS					935:937	2% Dextran sulfate sodium(DSS)	909:938	2% Dextran sulfate sodium(DSS)	909:938	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	4	94	theme	gut	743:745	arg1	microbiota					747:756	gut microbiota	743:756	gut microbiota	743:756	In the end, the mice were sacrificed, blood samples and colon tissues were collected for analysis of inflammation, and fecal samples were collected for 16 S rRNA sequencing of gut microbiota.
36401221	13	95	theme	harmful	1923:1929	arg1	bacteria					1968:1975	sulfate-reducing bacteria	1951:1975	sulfate-reducing bacteria	1951:1975	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	13	95	theme	harmful	1923:1929	arg1	bacteria					1931:1938	harmful bacteria	1923:1938	harmful bacteria	1923:1938	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	13	95	theme	harmful	1923:1929	arg1	Bacteroides					1981:1991	Bacteroides	1981:1991	Bacteroides	1981:1991	And changes in the composition of gut microbiota were characterized by the increase of harmful bacteria, including sulfate-reducing bacteria and Bacteroides, and the reduction of protective probiotics, butyrate-producing bacteria.
36401221	10	96	theme	gut	1583:1585	arg1	microbiota					1587:1596	gut microbiota	1583:1596	gut microbiota	1583:1596	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
36401221	5	97	theme	sodium	928:933	arg1	DSS					935:937	2% Dextran sulfate sodium(DSS)	909:938	2% Dextran sulfate sodium(DSS)	909:938	To establish ulcerative colitis mice model, at the beginning of the 4th week, mice maintained on the diet with or without triclosan were treated with 2% Dextran sulfate sodium(DSS) in drinking water for 1 week.
36401221	10	98	dep	changed	1542:1548	arg1	Both					1486:1489	Both	1486:1489	Both	1486:1489	Both in common and DSS-induced mice, triclosan exposure changed the diversity and composition of gut microbiota.
37216893	1	0	theme	persistent	260:269	arg1	activation					271:280	persistent activation	260:280	persistent activation	260:280	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	7	1	theme	NF-κB	1441:1445	arg1	pathway					1447:1453	the NF-κB pathway	1437:1453	the NF-κB pathway	1437:1453	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	8	2	from	inhibition	1544:1553	arg1	IL-6					1602:1605	IL-6	1602:1605	IL-6	1602:1605	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	2	from	inhibition	1544:1553	arg1	translocation					1696:1708	p65 nuclear translocation	1684:1708	p65 nuclear translocation	1684:1708	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	2	from	inhibition	1544:1553	arg1	cells					1719:1723	THP-1 cells	1713:1723	THP-1 cells	1713:1723	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	2	from	inhibition	1544:1553	arg1	interleukin					1587:1597	interleukin 6	1587:1599	interleukin 6 (IL-6)	1587:1606	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	4	3	theme	P.	879:880	arg1	bark					895:898	P. excelsa stem bark	879:898	P. excelsa stem bark	879:898	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	11	4	theme	tested	2392:2397	arg1	compounds					2399:2407	all tested compounds	2388:2407	all tested compounds	2388:2407	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	4	5	theme	stem	890:893	arg1	bark					895:898	P. excelsa stem bark	879:898	P. excelsa stem bark	879:898	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	7	6	theme	various	1419:1425	arg1	stages					1427:1432	various stages	1419:1432	various stages of the NF-κB pathway	1419:1453	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	9	7	theme	sulphation	1850:1859	arg1	influence					1837:1845	the influence	1833:1845	the influence of sulphation on the anti-inflammatory properties of naringenin derivatives	1833:1921	To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects.
37216893	4	8	theme	biological	948:957	arg1	activity					959:966	its biological activity	944:966	its biological activity	944:966	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	10	9	theme	anti-inflammatory	2101:2117	arg1	activities					2119:2128	potent anti-inflammatory activities	2094:2128	potent anti-inflammatory activities	2094:2128	Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1β production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production.
37216893	3	10	theme	action	768:773	arg1	mechanisms					754:763	mechanisms	754:763	mechanisms	754:763	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	3	10	theme	action	768:773	arg1	phytoconstituents					732:748	the phytoconstituents	728:748	the phytoconstituents	728:748	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	8	11	theme	nuclear	1688:1694	arg1	translocation					1696:1708	p65 nuclear translocation	1684:1708	p65 nuclear translocation	1684:1708	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	1	12	theme	health	216:221	arg1	states					223:228	aberrant health states	207:228	aberrant health states	207:228	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	10	13	theme	IL-6	2277:2280	arg1	production					2282:2291	IL-6 production	2277:2291	IL-6 production	2277:2291	Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1β production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production.
37216893	11	14	theme	naringenin	2500:2509	arg1	derivatives					2511:2521	naringenin derivatives	2500:2521	naringenin derivatives	2500:2521	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	4	15	from	composition	864:874	arg1	mechanisms					920:929	the mechanisms	916:929	the mechanisms underpinning its biological activity	916:966	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	9	16	theme	naringenin	1900:1909	arg1	derivatives					1911:1921	naringenin derivatives	1900:1921	naringenin derivatives	1900:1921	To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects.
37216893	3	17	theme	extract	619:625	arg1	effect					594:599	the inhibitory effect	579:599	the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α)	579:721	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	1	18	theme	inflammatory	132:143	arg1	response					145:152	The inflammatory response	128:152	The inflammatory response	128:152	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	1	18	theme	inflammatory	132:143	arg1	mechanism					165:173	a vital mechanism	157:173	a vital mechanism for repairing damage induced by aberrant health states or external insults	157:248	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	7	19	from	effects	1408:1414	arg1	stages					1427:1432	various stages	1419:1432	various stages of the NF-κB pathway	1419:1453	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	8	20	from	role	1758:1761	arg1	activity					1794:1801	the activity	1790:1801	the activity of naringenin (3)	1790:1819	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	3	21	from	effect	594:599	arg1	factor-alpha					702:713	tumour necrosis factor-alpha	686:713	tumour necrosis factor-alpha (TNF-α)	686:721	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	3	21	from	effect	594:599	arg1	TNF-α					716:720	TNF-α	716:720	TNF-α	716:720	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	6	22	theme	predominant	1042:1052	arg1	naringenin-8-sulphonate					1094:1116	naringenin-8-sulphonate	1094:1116	naringenin-8-sulphonate (1)	1094:1120	The predominant compound was isolated and identified as naringenin-8-sulphonate (1), while the identity of the second compound (compound 2) could not be determined.
37216893	6	22	theme	predominant	1042:1052	arg1	compound					1054:1061	The predominant compound	1038:1061	The predominant compound	1038:1061	The predominant compound was isolated and identified as naringenin-8-sulphonate (1), while the identity of the second compound (compound 2) could not be determined.
37216893	2	23	theme	ongoing	522:528	arg1	development					535:545	ongoing drug development	522:545	ongoing drug development	522:545	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	10	24	theme	IL-1β	2159:2163	arg1	production					2165:2174	IL-1β production	2159:2174	IL-1β production	2159:2174	Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1β production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production.
37216893	11	25	dep	P.	2343:2344	arg1	excelsa					2346:2352	excelsa	2346:2352	excelsa	2346:2352	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	0	26	theme	Sabine	75:80	arg1	bark					87:90	Parinari excelsa Sabine stem bark	58:90	Parinari excelsa Sabine stem bark	58:90	Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.
37216893	4	27	from	role	908:911	arg1	mechanisms					920:929	the mechanisms	916:929	the mechanisms underpinning its biological activity	916:966	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	1	28	theme	vital	159:163	arg1	response					145:152	The inflammatory response	128:152	The inflammatory response	128:152	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	1	28	theme	vital	159:163	arg1	mechanism					165:173	a vital mechanism	157:173	a vital mechanism for repairing damage induced by aberrant health states or external insults	157:248	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	11	29	theme	P.	2343:2344	arg1	extract					2354:2360	the P. excelsa extract	2339:2360	the P. excelsa extract	2339:2360	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	11	29	theme	P.	2343:2344	arg1	efficacious					2371:2381	efficacious	2371:2381	efficacious	2371:2381	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	4	30	theme	primary	797:803	arg1	objective					805:813	The primary objective	793:813	The primary objective of this study	793:827	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	8	31	theme	sulphur	1766:1772	arg1	substituents					1774:1785	sulphur substituents	1766:1785	sulphur substituents	1766:1785	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	5	32	theme	HPLC-DAD-ESI	1001:1012	arg1	analysis					1028:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	Two compounds were detected via HPLC-DAD-ESI(Ion Trap)-MS2 analysis.
37216893	8	33	theme	first	1519:1523	arg1	time					1525:1528	the first time	1515:1528	the first time	1515:1528	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	2	34	theme	associated	390:399	arg1	mediators					401:409	its associated mediators	386:409	its associated mediators	386:409	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	5	35	theme	Ion	1014:1016	arg1	analysis					1028:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	Two compounds were detected via HPLC-DAD-ESI(Ion Trap)-MS2 analysis.
37216893	2	36	theme	therapeutic	448:458	arg1	interventions					460:472	therapeutic interventions	448:472	therapeutic interventions aimed at modulating inflammation	448:505	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	11	37	theme	sulphation	2452:2461	arg1	role					2444:2447	the role	2440:2447	the role of sulphation in the anti-inflammatory activity of naringenin derivatives	2440:2521	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	0	38	theme	Anti-inflammatory	0:16	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of naringenin	0:38	Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.
37216893	7	39	theme	THP-1-derived	1330:1342	arg1	macrophages					1344:1354	THP-1-derived macrophages	1330:1354	THP-1-derived macrophages	1330:1354	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	8	40	theme	NF-κB	1558:1562	arg1	activity					1564:1571	NF-κB activity	1558:1571	NF-κB activity	1558:1571	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	3	41	theme	tumour	686:691	arg1	factor-alpha					702:713	tumour necrosis factor-alpha	686:713	tumour necrosis factor-alpha (TNF-α)	686:721	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	3	41	theme	tumour	686:691	arg1	TNF-α					716:720	TNF-α	716:720	TNF-α	716:720	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	1	42	theme	chronic	308:314	arg1	diseases					316:323	numerous chronic diseases	299:323	numerous chronic diseases	299:323	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	2	43	theme	β	351:351	arg1	pathway					374:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	0	44	theme	Parinari	58:65	arg1	bark					87:90	Parinari excelsa Sabine stem bark	58:90	Parinari excelsa Sabine stem bark	58:90	Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.
37216893	7	45	theme	cell-based	1290:1299	arg1	model					1314:1318	a cell-based inflammation model	1288:1318	a cell-based inflammation model	1288:1318	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	4	46	theme	study	823:827	arg1	objective					805:813	The primary objective	793:813	The primary objective of this study	793:827	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	2	47	theme	factor	338:343	arg1	pathway					374:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	8	48	theme	interleukin	1620:1630	arg1	production					1647:1656	interleukin 1 beta (IL-1β) production	1620:1656	interleukin 1 beta (IL-1β) production	1620:1656	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	1	49	theme	external	233:240	arg1	insults					242:248	external insults	233:248	external insults	233:248	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	2	50	theme	inflammatory	361:372	arg1	pathway					374:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	8	51	theme	beta	1634:1637	arg1	production					1647:1656	interleukin 1 beta (IL-1β) production	1620:1656	interleukin 1 beta (IL-1β) production	1620:1656	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	7	52	theme	anti-inflammatory	1253:1269	arg1	properties					1271:1280	anti-inflammatory properties	1253:1280	anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway	1253:1453	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	8	53	theme	naringenin	1806:1815	arg1	activity					1794:1801	the activity	1790:1801	the activity of naringenin (3)	1790:1819	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	2	54	theme	NF-κB	354:358	arg1	pathway					374:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	4	55	theme	bark	895:898	arg1	role					908:911	its role	904:911	its role in the mechanisms underpinning its biological activity	904:966	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	4	55	theme	bark	895:898	arg1	composition					864:874	the phytochemical composition	846:874	the phytochemical composition of P. excelsa stem bark	846:898	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	10	56	theme	Naringenin	2047:2056	arg1	derivatives					2058:2068	Naringenin derivatives 4 and 5	2047:2076	derivatives	2058:2068	Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1β production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production.
37216893	1	57	attach	linked	289:294	arg1	diseases					316:323	numerous chronic diseases	299:323	numerous chronic diseases	299:323	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	1	57	attach	linked	289:294	arg2	activation					271:280	persistent activation	260:280	persistent activation	260:280	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	7	58	theme	pathway	1447:1453	arg1	stages					1427:1432	various stages	1419:1432	various stages of the NF-κB pathway	1419:1453	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	1	59	theme	repairing	179:187	arg1	damage					189:194	repairing damage	179:194	repairing damage induced by aberrant health states or external insults	179:248	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	7	60	link	THP-1-derived	1330:1342	arg1	macrophages					1344:1354	THP-1-derived macrophages	1330:1354	THP-1-derived macrophages	1330:1354	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	8	61	from	reduction	1574:1582	arg1	IL-6					1602:1605	IL-6	1602:1605	IL-6	1602:1605	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	61	from	reduction	1574:1582	arg1	translocation					1696:1708	p65 nuclear translocation	1684:1708	p65 nuclear translocation	1684:1708	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	61	from	reduction	1574:1582	arg1	cells					1719:1723	THP-1 cells	1713:1723	THP-1 cells	1713:1723	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	61	from	reduction	1574:1582	arg1	interleukin					1587:1597	interleukin 6	1587:1599	interleukin 6 (IL-6)	1587:1606	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	4	62	theme	excelsa	882:888	arg1	bark					895:898	P. excelsa stem bark	879:898	P. excelsa stem bark	879:898	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	3	63	theme	inhibitory	583:592	arg1	effect					594:599	the inhibitory effect	579:599	the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α)	579:721	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	1	64	theme	aberrant	207:214	arg1	states					223:228	aberrant health states	207:228	aberrant health states	207:228	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	6	65	theme	compound	1156:1163	arg1	identity					1133:1140	the identity	1129:1140	the identity of the second compound (compound 2)	1129:1176	The predominant compound was isolated and identified as naringenin-8-sulphonate (1), while the identity of the second compound (compound 2) could not be determined.
37216893	9	66	theme	anti-inflammatory	1868:1884	arg1	properties					1886:1895	the anti-inflammatory properties	1864:1895	the anti-inflammatory properties of naringenin derivatives	1864:1921	To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects.
37216893	8	67	theme	p65	1684:1686	arg1	translocation					1696:1708	p65 nuclear translocation	1684:1708	p65 nuclear translocation	1684:1708	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	11	68	theme	anti-inflammatory	2470:2486	arg1	activity					2488:2495	the anti-inflammatory activity	2466:2495	the anti-inflammatory activity of naringenin derivatives	2466:2521	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	3	69	theme	hydroethanol	606:617	arg1	extract					619:625	a hydroethanol extract	604:625	a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae)	604:681	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	11	70	from	role	2444:2447	arg1	activity					2488:2495	the anti-inflammatory activity	2466:2495	the anti-inflammatory activity of naringenin derivatives	2466:2521	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	8	71	theme	biological	1474:1483	arg1	activity					1485:1492	activity	1485:1492	activity	1485:1492	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	9	72	theme	derivatives	1911:1921	arg1	properties					1886:1895	the anti-inflammatory properties	1864:1895	the anti-inflammatory properties of naringenin derivatives	1864:1921	To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects.
37216893	8	73	from	TNF-α	1609:1613	arg1	IL-6					1602:1605	IL-6	1602:1605	IL-6	1602:1605	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	73	from	TNF-α	1609:1613	arg1	translocation					1696:1708	p65 nuclear translocation	1684:1708	p65 nuclear translocation	1684:1708	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	73	from	TNF-α	1609:1613	arg1	cells					1719:1723	THP-1 cells	1713:1723	THP-1 cells	1713:1723	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	73	from	TNF-α	1609:1613	arg1	interleukin					1587:1597	interleukin 6	1587:1599	interleukin 6 (IL-6)	1587:1606	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	74	theme	THP-1	1713:1717	arg1	cells					1719:1723	THP-1 cells	1713:1723	THP-1 cells	1713:1723	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	11	75	theme	derivatives	2511:2521	arg1	activity					2488:2495	the anti-inflammatory activity	2466:2495	the anti-inflammatory activity of naringenin derivatives	2466:2521	Collectively, the findings demonstrated that the P. excelsa extract was more efficacious than all tested compounds, while providing insights into the role of sulphation in the anti-inflammatory activity of naringenin derivatives.
37216893	0	76	theme	stem	82:85	arg1	bark					87:90	Parinari excelsa Sabine stem bark	58:90	Parinari excelsa Sabine stem bark	58:90	Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.
37216893	9	77	from	influence	1837:1845	arg1	properties					1886:1895	the anti-inflammatory properties	1864:1895	the anti-inflammatory properties of naringenin derivatives	1864:1921	To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects.
37216893	3	78	attach	derived	627:633	arg1	Chrysobalanaceae					665:680	Chrysobalanaceae	665:680	Chrysobalanaceae	665:680	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	3	78	attach	derived	627:633	arg1	Sabine					657:662	Sabine	657:662	Sabine	657:662	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	3	78	attach	derived	627:633	arg2	extract					619:625	a hydroethanol extract	604:625	a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae)	604:681	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	0	79	theme	semi-synthetic	100:113	arg1	derivatives					115:125	its semi-synthetic derivatives	96:125	its semi-synthetic derivatives	96:125	Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.
37216893	8	80	theme	potential	1748:1756	arg1	role					1758:1761	the potential role	1744:1761	the potential role of sulphur substituents in the activity of naringenin (3)	1744:1819	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	10	81	theme	potent	2094:2099	arg1	activities					2119:2128	potent anti-inflammatory activities	2094:2128	potent anti-inflammatory activities	2094:2128	Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1β production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production.
37216893	2	82	theme	drug	530:533	arg1	development					535:545	ongoing drug development	522:545	ongoing drug development	522:545	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	8	83	theme	substituents	1774:1785	arg1	role					1758:1761	the potential role	1744:1761	the potential role of sulphur substituents in the activity of naringenin (3)	1744:1819	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	10	84	theme	p65	2203:2205	arg1	translocation					2207:2219	p65 translocation	2203:2219	p65 translocation	2203:2219	Naringenin derivatives 4 and 5 did not display potent anti-inflammatory activities; however, compound 4 reduced IL-1β production, and compound 5 diminished p65 translocation, with both exhibiting the capacity to inhibit TNF-α and IL-6 production.
37216893	8	85	from	production	1647:1656	arg1	IL-6					1602:1605	IL-6	1602:1605	IL-6	1602:1605	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	85	from	production	1647:1656	arg1	translocation					1696:1708	p65 nuclear translocation	1684:1708	p65 nuclear translocation	1684:1708	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	85	from	production	1647:1656	arg1	cells					1719:1723	THP-1 cells	1713:1723	THP-1 cells	1713:1723	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	85	from	production	1647:1656	arg1	interleukin					1587:1597	interleukin 6	1587:1599	interleukin 6 (IL-6)	1587:1606	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	0	86	theme	naringenin	29:38	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of naringenin	0:38	Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.
37216893	2	87	theme	critical	427:434	arg1	targets					436:442	critical targets	427:442	critical targets for therapeutic interventions aimed at modulating inflammation	427:505	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	8	88	theme	activity	1564:1571	arg1	reduction					1574:1582	reduction	1574:1582	reduction in interleukin 6 (IL-6)	1574:1606	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	88	theme	activity	1564:1571	arg1	inhibition					1544:1553	inhibition	1544:1553	inhibition of NF-κB activity	1544:1571	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	88	theme	activity	1564:1571	arg1	production					1647:1656	interleukin 1 beta (IL-1β) production	1620:1656	interleukin 1 beta (IL-1β) production	1620:1656	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	88	theme	activity	1564:1571	arg1	decrease					1672:1679	a decrease	1670:1679	a decrease in p65 nuclear translocation in THP-1 cells	1670:1723	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	88	theme	activity	1564:1571	arg1	TNF-α					1609:1613	TNF-α	1609:1613	TNF-α	1609:1613	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	1	89	theme	numerous	299:306	arg1	diseases					316:323	numerous chronic diseases	299:323	numerous chronic diseases	299:323	The inflammatory response is a vital mechanism for repairing damage induced by aberrant health states or external insults; however, persistent activation can be linked to numerous chronic diseases.
37216893	7	90	theme	inflammation	1301:1312	arg1	model					1314:1318	a cell-based inflammation model	1288:1318	a cell-based inflammation model	1288:1318	Both compound 1 and the extract were assessed for anti-inflammatory properties using a cell-based inflammation model, in which THP-1-derived macrophages were stimulated with LPS to examine the treatments' effects on various stages of the NF-κB pathway.
37216893	9	91	theme	anti-inflammatory	2020:2036	arg1	effects					2038:2044	their anti-inflammatory effects	2014:2044	their anti-inflammatory effects	2014:2044	To explore the influence of sulphation on the anti-inflammatory properties of naringenin derivatives, we synthesized naringenin-4'-O-sulphate (4) and naringenin-7-O-sulphate (5) and evaluated their anti-inflammatory effects.
37216893	0	92	theme	excelsa	67:73	arg1	bark					87:90	Parinari excelsa Sabine stem bark	58:90	Parinari excelsa Sabine stem bark	58:90	Anti-inflammatory effects of naringenin 8-sulphonate from Parinari excelsa Sabine stem bark and its semi-synthetic derivatives.
37216893	6	93	theme	second	1149:1154	arg1	compound					1166:1173	compound 2	1166:1175	compound 2	1166:1175	The predominant compound was isolated and identified as naringenin-8-sulphonate (1), while the identity of the second compound (compound 2) could not be determined.
37216893	6	93	theme	second	1149:1154	arg1	compound					1156:1163	the second compound	1145:1163	the second compound (compound 2)	1145:1176	The predominant compound was isolated and identified as naringenin-8-sulphonate (1), while the identity of the second compound (compound 2) could not be determined.
37216893	4	94	theme	phytochemical	850:862	arg1	composition					864:874	the phytochemical composition	846:874	the phytochemical composition of P. excelsa stem bark	846:898	The primary objective of this study was to elucidate the phytochemical composition of P. excelsa stem bark and its role in the mechanisms underpinning its biological activity.
37216893	2	95	theme	kappa	345:349	arg1	pathway					374:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	5	96	theme	Trap	1018:1021	arg1	analysis					1028:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	Two compounds were detected via HPLC-DAD-ESI(Ion Trap)-MS2 analysis.
37216893	3	97	theme	necrosis	693:700	arg1	factor-alpha					702:713	tumour necrosis factor-alpha	686:713	tumour necrosis factor-alpha (TNF-α)	686:721	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	3	97	theme	necrosis	693:700	arg1	TNF-α					716:720	TNF-α	716:720	TNF-α	716:720	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
37216893	2	98	theme	nuclear	330:336	arg1	pathway					374:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway	326:380	The nuclear factor kappa β (NF-κB) inflammatory pathway and its associated mediators have emerged as critical targets for therapeutic interventions aimed at modulating inflammation, necessitating ongoing drug development.
37216893	5	99	theme	-MS2	1023:1026	arg1	analysis					1028:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	HPLC-DAD-ESI(Ion Trap)-MS2 analysis	1001:1035	Two compounds were detected via HPLC-DAD-ESI(Ion Trap)-MS2 analysis.
37216893	8	100	from	decrease	1672:1679	arg1	IL-6					1602:1605	IL-6	1602:1605	IL-6	1602:1605	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	100	from	decrease	1672:1679	arg1	translocation					1696:1708	p65 nuclear translocation	1684:1708	p65 nuclear translocation	1684:1708	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	100	from	decrease	1672:1679	arg1	cells					1719:1723	THP-1 cells	1713:1723	THP-1 cells	1713:1723	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	8	100	from	decrease	1672:1679	arg1	interleukin					1587:1597	interleukin 6	1587:1599	interleukin 6 (IL-6)	1587:1606	Compound 1, whose biological activity is reported here for the first time, demonstrated inhibition of NF-κB activity, reduction in interleukin 6 (IL-6), TNF-α, and interleukin 1 beta (IL-1β) production, as well as a decrease in p65 nuclear translocation in THP-1 cells, thus highlighting the potential role of sulphur substituents in the activity of naringenin (3).
37216893	3	101	theme	Previous	548:555	arg1	studies					557:563	Previous studies	548:563	Previous studies	548:563	Previous studies have reported the inhibitory effect of a hydroethanol extract derived from Parinari excelsa Sabine (Chrysobalanaceae) on tumour necrosis factor-alpha (TNF-α), but the phytoconstituents and mechanisms of action remained elusive.
36282570	3	0	theme	smooth	304:309	arg1	spores					322:327	smooth long chain spores	304:327	smooth long chain spores	304:327	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	2	1	theme	strain	132:137	arg1	T5T					139:141	strain T5T	132:141	strain T5T	132:141	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	12	2	theme	solincola	1672:1680	arg1	sp					1682:1683	the name Streptomyces solincola sp	1650:1683	the name Streptomyces solincola sp	1650:1683	Based on these polyphasic data, strain T5T represents a novel species, for which the name Streptomyces solincola sp.
36282570	6	3	theme	acid	659:662	arg1	isomer					664:669	The ll-diaminopimelic acid isomer	637:669	The ll-diaminopimelic acid isomer	637:669	The ll-diaminopimelic acid isomer was detected in the cell wall.
36282570	9	4	theme	rRNA	987:990	arg1	sequences					997:1005	the 16S rRNA gene sequences	979:1005	the 16S rRNA gene sequences	979:1005	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	9	5	with	Streptomycetaceae	1072:1088	arg1	similarity					1130:1139	the highest 16S rRNA gene sequence similarity	1095:1139	the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1095:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	3	6	theme	long	311:314	arg1	spores					322:327	smooth long chain spores	304:327	smooth long chain spores	304:327	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	9	7	theme	rRNA	1111:1114	arg1	similarity					1130:1139	the highest 16S rRNA gene sequence similarity	1095:1139	the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1095:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	5	8	theme	strain	585:590	arg1	T5T					592:594	strain T5T	585:594	strain T5T	585:594	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	9	9	theme	gene	1116:1119	arg1	similarity					1130:1139	the highest 16S rRNA gene sequence similarity	1095:1139	the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1095:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	1	10	from	soil	47:50	arg1	Malaysia					55:62	Malaysia	55:62	Malaysia	55:62	nov., isolated from soil in Malaysia.
36282570	10	11	theme	overall	1189:1195	arg1	indices					1216:1222	The overall genome relatedness indices	1185:1222	The overall genome relatedness indices	1185:1222	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	5	12	theme	T5T	592:594	arg1	hydrolysate					570:580	The whole-cell sugar hydrolysate	549:580	The whole-cell sugar hydrolysate of strain T5T	549:594	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	9	13	theme	sequence	1121:1128	arg1	similarity					1130:1139	the highest 16S rRNA gene sequence similarity	1095:1139	the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1095:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	8	14	dep	anteiso-C17 	892:903	arg1	 0					935:936	 0	935:936	 0	935:936	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	8	14	dep	anteiso-C17 	892:903	arg1	 0					905:906	 0	905:906	 0	905:906	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	8	14	dep	anteiso-C17 	892:903	arg1	 0					918:919	 0	918:919	 0	918:919	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	8	14	dep	anteiso-C17 	892:903	arg1	 0					951:952	 0	951:952	 0	951:952	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	8	14	dep	anteiso-C17 	892:903	arg1	iso-C14 					942:949	iso-C14 	942:949	iso-C14 	942:949	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	3	15	theme	light	354:358	arg1	mycelia					385:391	light yellowish-pink substrate mycelia	354:391	light yellowish-pink substrate mycelia	354:391	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	4	16	theme	tolerated	452:460	arg1	NaCl					478:481	tolerated up to 9 % (w/v) NaCl	452:481	tolerated up to 9 % (w/v) NaCl	452:481	Strain T5T grew at 15-37 °C, pH 6-11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl.
36282570	9	17	theme	family	1065:1070	arg1	Streptomycetaceae					1072:1088	the family Streptomycetaceae	1061:1088	the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1061:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	4	18	theme	Strain	410:415	arg1	T5T					417:419	Strain T5T	410:419	Strain T5T	410:419	Strain T5T grew at 15-37 °C, pH 6-11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl.
36282570	11	19	theme	strain	1404:1409	arg1	T5T					1411:1413	strain T5T	1404:1413	strain T5T	1404:1413	Phylogeny analyses showed that strain T5T was closely related to Streptomyces fradiae, Streptomyces lavendofoliae, Streptomyces lichenis, Streptomyces roseolilacinus and Streptomyces somaliensis.
36282570	6	20	theme	ll-diaminopimelic	641:657	arg1	isomer					664:669	The ll-diaminopimelic acid isomer	637:669	The ll-diaminopimelic acid isomer	637:669	The ll-diaminopimelic acid isomer was detected in the cell wall.
36282570	2	21	from	hillslope	199:207	arg1	Malaysia					222:229	Malaysia	222:229	Malaysia	222:229	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	2	21	from	hillslope	199:207	arg1	Selangor					212:219	Selangor	212:219	Selangor	212:219	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	7	22	dep	Diphosphatidylglycerol	702:723	arg1	phosphatidylethanolamine					747:770	phosphatidylethanolamine	747:770	phosphatidylethanolamine	747:770	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	7	22	dep	Diphosphatidylglycerol	702:723	arg1	phosphatidylcholine					726:744	phosphatidylcholine	726:744	phosphatidylcholine	726:744	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	7	22	dep	Diphosphatidylglycerol	702:723	arg1	phosphatidylinositol					798:817	phosphatidylinositol	798:817	phosphatidylinositol	798:817	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	7	22	dep	Diphosphatidylglycerol	702:723	arg1	phosphatidylglycerol					773:792	phosphatidylglycerol	773:792	phosphatidylglycerol	773:792	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	10	23	theme	genome	1197:1202	arg1	indices					1216:1222	The overall genome relatedness indices	1185:1222	The overall genome relatedness indices	1185:1222	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	7	24	theme	predominant	840:850	arg1	lipids					858:863	the predominant polar lipids	836:863	the predominant polar lipids	836:863	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	7	24	theme	predominant	840:850	arg1	Diphosphatidylglycerol					702:723	Diphosphatidylglycerol	702:723	Diphosphatidylglycerol	702:723	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	5	25	theme	whole-cell	553:562	arg1	hydrolysate					570:580	The whole-cell sugar hydrolysate	549:580	The whole-cell sugar hydrolysate of strain T5T	549:594	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	9	26	theme	Streptomyces	1144:1155	arg1	LCR6-01T					1166:1173	Streptomyces lichenis LCR6-01T	1144:1173	Streptomyces lichenis LCR6-01T (99.0 %)	1144:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	9	26	theme	Streptomyces	1144:1155	arg1	%					1181:1181	99.0 %	1176:1181	99.0 %	1176:1181	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	6	27	located	detected	675:682	arg2	isomer					664:669	The ll-diaminopimelic acid isomer	637:669	The ll-diaminopimelic acid isomer	637:669	The ll-diaminopimelic acid isomer was detected in the cell wall.
36282570	6	27	located	detected	675:682	arg1	wall					696:699	the cell wall	687:699	the cell wall	687:699	The ll-diaminopimelic acid isomer was detected in the cell wall.
36282570	9	28	theme	strain	1022:1027	arg1	T5T					1029:1031	strain T5T	1022:1031	strain T5T	1022:1031	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	14	29	theme	5137T=	1734:1739	arg1	42166T					1745:1750	=TBRC 5137T= DSM 42166T	1728:1750	=TBRC 5137T= DSM 42166T	1728:1750	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	14	29	theme	5137T=	1734:1739	arg1	T5T					1723:1725	T5T	1723:1725	T5T (=TBRC 5137T= DSM 42166T)	1723:1751	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	0	30	theme	solincola	13:21	arg1	sp					23:24	Streptomyces solincola sp	0:24	Streptomyces solincola sp.	0:25	Streptomyces solincola sp.
36282570	5	31	theme	sugar	564:568	arg1	hydrolysate					570:580	The whole-cell sugar hydrolysate	549:580	The whole-cell sugar hydrolysate of strain T5T	549:594	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	3	32	with	mycelia	291:297	arg1	spores					322:327	smooth long chain spores	304:327	smooth long chain spores	304:327	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	0	33	theme	Streptomyces	0:11	arg1	sp					23:24	Streptomyces solincola sp	0:24	Streptomyces solincola sp.	0:25	Streptomyces solincola sp.
36282570	9	34	dep	Streptomyces	1144:1155	arg1	lichenis					1157:1164	lichenis	1157:1164	lichenis	1157:1164	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	10	35	theme	DNA-DNA	1350:1356	arg1	hybridization					1358:1370	33.7 % digital DNA-DNA hybridization	1335:1370	33.7 % digital DNA-DNA hybridization	1335:1370	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	10	36	theme	relatedness	1204:1214	arg1	indices					1216:1222	The overall genome relatedness indices	1185:1222	The overall genome relatedness indices	1185:1222	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	8	37	theme	fatty	875:879	arg1	anteiso-C17 					892:903	anteiso-C17 	892:903	anteiso-C17 	892:903	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	8	37	theme	fatty	875:879	arg1	acids					881:885	The main fatty acids	866:885	The main fatty acids	866:885	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	10	38	theme	89.4 	1296:1300	arg1	%					1301:1301	%	1301:1301	%	1301:1301	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	3	39	theme	yellowish-pink	360:373	arg1	mycelia					385:391	light yellowish-pink substrate mycelia	354:391	light yellowish-pink substrate mycelia	354:391	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	4	40	theme	optimal	489:495	arg1	growth					497:502	optimal growth	489:502	optimal growth occurring at 28 °C, pH 6-9	489:529	Strain T5T grew at 15-37 °C, pH 6-11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl.
36282570	10	41	theme	digital	1342:1348	arg1	hybridization					1358:1370	33.7 % digital DNA-DNA hybridization	1335:1370	33.7 % digital DNA-DNA hybridization	1335:1370	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	8	42	theme	main	870:873	arg1	anteiso-C17 					892:903	anteiso-C17 	892:903	anteiso-C17 	892:903	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	8	42	theme	main	870:873	arg1	acids					881:885	The main fatty acids	866:885	The main fatty acids	866:885	The main fatty acids were anteiso-C17 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C14 : 0.
36282570	14	43	theme	type	1708:1711	arg1	T5T					1723:1725	T5T	1723:1725	T5T (=TBRC 5137T= DSM 42166T)	1723:1751	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	14	43	theme	type	1708:1711	arg1	strain					1713:1718	The type strain	1704:1718	The type strain	1704:1718	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	3	44	theme	substrate	375:383	arg1	mycelia					385:391	light yellowish-pink substrate mycelia	354:391	light yellowish-pink substrate mycelia	354:391	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	12	45	theme	strain	1601:1606	arg1	T5T					1608:1610	strain T5T	1601:1610	strain T5T	1601:1610	Based on these polyphasic data, strain T5T represents a novel species, for which the name Streptomyces solincola sp.
36282570	3	46	theme	pale	264:267	arg1	mycelia					291:297	pale yellowish-pink aerial mycelia	264:297	pale yellowish-pink aerial mycelia with smooth long chain spores	264:327	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	10	47	theme	%	1340:1340	arg1	hybridization					1358:1370	33.7 % digital DNA-DNA hybridization	1335:1370	33.7 % digital DNA-DNA hybridization	1335:1370	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	11	48	theme	Phylogeny	1373:1381	arg1	analyses					1383:1390	Phylogeny analyses	1373:1390	Phylogeny analyses	1373:1390	Phylogeny analyses showed that strain T5T was closely related to Streptomyces fradiae, Streptomyces lavendofoliae, Streptomyces lichenis, Streptomyces roseolilacinus and Streptomyces somaliensis.
36282570	10	49	theme	related	1250:1256	arg1	LCR6-01T					1282:1289	S. lichenis LCR6-01T	1270:1289	S. lichenis LCR6-01T	1270:1289	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	10	49	theme	related	1250:1256	arg1	species					1258:1264	the closest related species	1238:1264	the closest related species	1238:1264	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	7	50	theme	polar	852:856	arg1	lipids					858:863	the predominant polar lipids	836:863	the predominant polar lipids	836:863	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	7	50	theme	polar	852:856	arg1	Diphosphatidylglycerol					702:723	Diphosphatidylglycerol	702:723	Diphosphatidylglycerol	702:723	Diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylinositol were found to be the predominant polar lipids.
36282570	9	51	theme	gene	992:995	arg1	sequences					997:1005	the 16S rRNA gene sequences	979:1005	the 16S rRNA gene sequences	979:1005	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	10	52	theme	closest	1242:1248	arg1	LCR6-01T					1282:1289	S. lichenis LCR6-01T	1270:1289	S. lichenis LCR6-01T	1270:1289	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	10	52	theme	closest	1242:1248	arg1	species					1258:1264	the closest related species	1238:1264	the closest related species	1238:1264	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	12	53	theme	name	1654:1657	arg1	sp					1682:1683	the name Streptomyces solincola sp	1650:1683	the name Streptomyces solincola sp	1650:1683	Based on these polyphasic data, strain T5T represents a novel species, for which the name Streptomyces solincola sp.
36282570	10	54	theme	average	1303:1309	arg1	identity					1322:1329	89.4 % average nucleotide identity	1296:1329	89.4 % average nucleotide identity	1296:1329	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	2	55	theme	soil	164:167	arg1	sample					169:174	a soil sample	162:174	a soil sample collected at a highway hillslope in Selangor, Malaysia	162:229	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	14	56	theme	=TBRC	1728:1732	arg1	42166T					1745:1750	=TBRC 5137T= DSM 42166T	1728:1750	=TBRC 5137T= DSM 42166T	1728:1750	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	14	56	theme	=TBRC	1728:1732	arg1	T5T					1723:1725	T5T	1723:1725	T5T (=TBRC 5137T= DSM 42166T)	1723:1751	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	3	57	theme	oatmeal	396:402	arg1	agar					404:407	oatmeal agar	396:407	oatmeal agar	396:407	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	2	58	theme	Gram-stain-positive	84:102	arg1	actinobacterium					104:118	A urease-producing Gram-stain-positive actinobacterium	65:118	A urease-producing Gram-stain-positive actinobacterium	65:118	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	12	59	theme	polyphasic	1584:1593	arg1	data					1595:1598	these polyphasic data	1578:1598	these polyphasic data	1578:1598	Based on these polyphasic data, strain T5T represents a novel species, for which the name Streptomyces solincola sp.
36282570	6	60	theme	cell	691:694	arg1	wall					696:699	the cell wall	687:699	the cell wall	687:699	The ll-diaminopimelic acid isomer was detected in the cell wall.
36282570	2	61	attach	isolated	148:155	arg2	actinobacterium					104:118	A urease-producing Gram-stain-positive actinobacterium	65:118	A urease-producing Gram-stain-positive actinobacterium	65:118	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	2	61	attach	isolated	148:155	arg1	sample					169:174	a soil sample	162:174	a soil sample collected at a highway hillslope in Selangor, Malaysia	162:229	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	2	62	theme	urease-producing	67:82	arg1	actinobacterium					104:118	A urease-producing Gram-stain-positive actinobacterium	65:118	A urease-producing Gram-stain-positive actinobacterium	65:118	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	9	63	theme	Streptomycetaceae	1072:1088	arg1	Streptomyces					1045:1056	Streptomyces	1045:1056	Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1045:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	9	64	theme	Comparative	955:965	arg1	analysis					967:974	Comparative analysis	955:974	Comparative analysis of the 16S rRNA gene sequences	955:1005	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	4	65	dep	9 	468:469	arg1	to					465:466	to	465:466	to	465:466	Strain T5T grew at 15-37 °C, pH 6-11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl.
36282570	3	66	theme	yellowish-pink	269:282	arg1	mycelia					291:297	pale yellowish-pink aerial mycelia	264:297	pale yellowish-pink aerial mycelia with smooth long chain spores	264:327	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	2	67	theme	highway	191:197	arg1	hillslope					199:207	a highway hillslope	189:207	a highway hillslope in Selangor, Malaysia	189:229	A urease-producing Gram-stain-positive actinobacterium, designated strain T5T, was isolated from a soil sample collected at a highway hillslope in Selangor, Malaysia.
36282570	10	68	theme	33.7 	1335:1339	arg1	%					1340:1340	%	1340:1340	%	1340:1340	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	10	69	theme	%	1301:1301	arg1	identity					1322:1329	89.4 % average nucleotide identity	1296:1329	89.4 % average nucleotide identity	1296:1329	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	5	70	contain	contained	596:604	arg2	galactose					606:614	galactose	606:614	galactose	606:614	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	5	70	contain	contained	596:604	arg1	hydrolysate					570:580	The whole-cell sugar hydrolysate	549:580	The whole-cell sugar hydrolysate of strain T5T	549:594	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	5	70	contain	contained	596:604	arg2	ribose					629:634	ribose	629:634	ribose	629:634	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	5	70	contain	contained	596:604	arg2	glucose					617:623	glucose	617:623	glucose	617:623	The whole-cell sugar hydrolysate of strain T5T contained galactose, glucose and ribose.
36282570	3	71	theme	aerial	284:289	arg1	mycelia					291:297	pale yellowish-pink aerial mycelia	264:297	pale yellowish-pink aerial mycelia with smooth long chain spores	264:327	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	12	72	theme	novel	1625:1629	arg1	species					1631:1637	a novel species	1623:1637	a novel species	1623:1637	Based on these polyphasic data, strain T5T represents a novel species, for which the name Streptomyces solincola sp.
36282570	10	73	theme	nucleotide	1311:1320	arg1	identity					1322:1329	89.4 % average nucleotide identity	1296:1329	89.4 % average nucleotide identity	1296:1329	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	10	74	dep	S.	1270:1271	arg1	lichenis					1273:1280	lichenis	1273:1280	lichenis	1273:1280	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	14	75	theme	DSM	1741:1743	arg1	42166T					1745:1750	=TBRC 5137T= DSM 42166T	1728:1750	=TBRC 5137T= DSM 42166T	1728:1750	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	14	75	theme	DSM	1741:1743	arg1	T5T					1723:1725	T5T	1723:1725	T5T (=TBRC 5137T= DSM 42166T)	1723:1751	The type strain is T5T (=TBRC 5137T= DSM 42166T).
36282570	10	76	theme	S.	1270:1271	arg1	LCR6-01T					1282:1289	S. lichenis LCR6-01T	1270:1289	S. lichenis LCR6-01T	1270:1289	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	10	76	theme	S.	1270:1271	arg1	species					1258:1264	the closest related species	1238:1264	the closest related species	1238:1264	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	3	77	theme	chain	316:320	arg1	spores					322:327	smooth long chain spores	304:327	smooth long chain spores	304:327	The strain was found to produce pale yellowish-pink aerial mycelia with smooth long chain spores and extensively branched light yellowish-pink substrate mycelia on oatmeal agar.
36282570	9	78	theme	sequences	997:1005	arg1	analysis					967:974	Comparative analysis	955:974	Comparative analysis of the 16S rRNA gene sequences	955:1005	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	9	79	theme	highest	1099:1105	arg1	similarity					1130:1139	the highest 16S rRNA gene sequence similarity	1095:1139	the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1095:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	4	80	theme	%	470:470	arg1	NaCl					478:481	tolerated up to 9 % (w/v) NaCl	452:481	tolerated up to 9 % (w/v) NaCl	452:481	Strain T5T grew at 15-37 °C, pH 6-11, and tolerated up to 9 % (w/v) NaCl, with optimal growth occurring at 28 °C, pH 6-9 and without NaCl.
36282570	10	81	with	LCR6-01T	1282:1289	arg1	identity					1322:1329	89.4 % average nucleotide identity	1296:1329	89.4 % average nucleotide identity	1296:1329	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	10	81	with	LCR6-01T	1282:1289	arg1	hybridization					1358:1370	33.7 % digital DNA-DNA hybridization	1335:1370	33.7 % digital DNA-DNA hybridization	1335:1370	The overall genome relatedness indices revealed that the closest related species was S. lichenis LCR6-01T with 89.4 % average nucleotide identity and 33.7 % digital DNA-DNA hybridization.
36282570	9	82	theme	16S	1107:1109	arg1	similarity					1130:1139	the highest 16S rRNA gene sequence similarity	1095:1139	the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %)	1095:1182	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	9	83	theme	16S	983:985	arg1	sequences					997:1005	the 16S rRNA gene sequences	979:1005	the 16S rRNA gene sequences	979:1005	Comparative analysis of the 16S rRNA gene sequences indicated that strain T5T belonged to Streptomyces of the family Streptomycetaceae with the highest 16S rRNA gene sequence similarity to Streptomyces lichenis LCR6-01T (99.0 %).
36282570	12	84	theme	Streptomyces	1659:1670	arg1	sp					1682:1683	the name Streptomyces solincola sp	1650:1683	the name Streptomyces solincola sp	1650:1683	Based on these polyphasic data, strain T5T represents a novel species, for which the name Streptomyces solincola sp.
36150411	2	0	theme	ASIP	274:277	arg1	role					266:269	the potential role	252:269	the potential role of ASIP in the regulation of additional physiological pathways in the skin	252:344	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	2	0	theme	ASIP	274:277	arg1	unknown					349:355	unknown	349:355	unknown	349:355	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	4	1	theme	ASIP	617:620	arg1	role					609:612	the putative role	596:612	the putative role of ASIP in regulating the immune system of skin	596:660	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	10	2	theme	integrity	1759:1767	arg1	indicator					1731:1739	an indicator	1728:1739	an indicator of reduced tissue integrity and barrier function	1728:1788	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	10	2	theme	integrity	1759:1767	arg1	resistance					1716:1725	reduced epithelial resistance	1697:1725	reduced epithelial resistance	1697:1725	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	1	3	theme	receptors	172:180	arg1	antagonist					125:134	a competitive antagonist	111:134	a competitive antagonist	111:134	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	1	3	theme	receptors	172:180	arg1	agonist					148:154	inverse agonist	140:154	inverse agonist	140:154	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	1	3	theme	receptors	172:180	arg1	protein					83:89	The agouti-signaling protein	62:89	The agouti-signaling protein (ASIP)	62:96	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	10	4	theme	reduced	1744:1750	arg1	integrity					1759:1767	reduced tissue integrity	1744:1767	reduced tissue integrity	1744:1767	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	3	5	theme	immune	555:560	arg1	proteins					571:578	many immune effector proteins	550:578	many immune effector proteins	550:578	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	1	6	theme	pigmentation	212:223	arg1	patterns					225:232	dorsal-ventral pigmentation patterns	197:232	dorsal-ventral pigmentation patterns in fish	197:240	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	8	7	theme	stressful	1390:1398	arg1	conditions					1400:1409	stressful conditions	1390:1409	stressful conditions	1390:1409	ASIPzf also exhibit higher protein concentration under crowding conditions suggesting an increased mucus production under stressful conditions.
36150411	10	8	theme	barrier	1773:1779	arg1	function					1781:1788	barrier function	1773:1788	barrier function	1773:1788	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	7	9	theme	microorganism	1244:1256	arg1	adhesion					1258:1265	microorganism adhesion	1244:1265	microorganism adhesion	1244:1265	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	7	10	theme	mucus	1126:1130	arg1	composition					1132:1142	the mucus composition	1122:1142	the mucus composition	1122:1142	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	11	11	theme	inflammatory	1952:1963	arg1	response					1965:1972	an increased inflammatory response	1939:1972	an increased inflammatory response that can lead to increased skin sensitivity to external infections	1939:2039	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	2	12	from	regulation	286:295	arg1	skin					341:344	the skin	337:344	the skin	337:344	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	4	13	theme	immune	640:645	arg1	system					647:652	the immune system	636:652	the immune system of skin	636:660	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	1	14	theme	inverse	140:146	arg1	agonist					148:154	inverse agonist	140:154	inverse agonist	140:154	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	0	15	from	Role	0:3	arg1	physiology					50:59	zebrafish skin physiology	35:59	zebrafish skin physiology	35:59	Role of the melanocortin system in zebrafish skin physiology.
36150411	4	16	theme	zebrafish	699:707	arg1	model					709:713	a transgenic zebrafish model	686:713	a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf)	686:752	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	7	17	theme	layers	1102:1107	arg1	number					1087:1092	the lower number	1077:1092	the lower number of cell layers	1077:1107	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	11	18	theme	increased	1991:1999	arg1	sensitivity					2006:2016	increased skin sensitivity	1991:2016	increased skin sensitivity to external infections	1991:2039	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	9	19	theme	faster	1488:1493	arg1	response					1512:1519	a faster pro-inflammatory response	1486:1519	a faster pro-inflammatory response	1486:1519	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	1	20	theme	agouti-signaling	66:81	arg1	antagonist					125:134	a competitive antagonist	111:134	a competitive antagonist	111:134	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	1	20	theme	agouti-signaling	66:81	arg1	ASIP					92:95	ASIP	92:95	ASIP	92:95	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	1	20	theme	agouti-signaling	66:81	arg1	protein					83:89	The agouti-signaling protein	62:89	The agouti-signaling protein (ASIP)	62:96	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	3	21	theme	immune	395:400	arg1	function					402:409	the immune function	391:409	the immune function	391:409	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	7	22	theme	lower	1081:1085	arg1	number					1087:1092	the lower number	1077:1092	the lower number of cell layers	1077:1107	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	9	23	theme	increased	1525:1533	arg1	expression					1541:1550	increased mucin expression	1525:1550	increased mucin expression	1525:1550	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	6	24	theme	mucous	960:965	arg1	cells					967:971	mucous cells	960:971	mucous cells	960:971	A moderate hypertrophy of mucous cells was also found in ASIPzf.
36150411	11	25	theme	increased	1942:1950	arg1	response					1965:1972	an increased inflammatory response	1939:1972	an increased inflammatory response that can lead to increased skin sensitivity to external infections	1939:2039	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	8	26	theme	protein	1295:1301	arg1	concentration					1303:1315	higher protein concentration	1288:1315	higher protein concentration	1288:1315	ASIPzf also exhibit higher protein concentration under crowding conditions suggesting an increased mucus production under stressful conditions.
36150411	3	27	theme	physical	424:431	arg1	limitation					433:442	a physical limitation	422:442	a physical limitation against infestation	422:462	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	5	28	theme	ASIPzf	878:883	arg1	thinner					889:895	thinner	889:895	thinner	889:895	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	5	28	theme	ASIPzf	878:883	arg1	skin					870:873	the ventral skin	858:873	the ventral skin of ASIPzf	858:883	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	5	29	theme	asip1	808:812	arg1	overexpression					814:827	asip1 overexpression	808:827	asip1 overexpression	808:827	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	11	30	theme	skin	1895:1898	arg1	integrity					1911:1919	reduced skin epithelial integrity	1887:1919	reduced skin epithelial integrity	1887:1919	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	10	31	theme	Electrophysiological	1625:1644	arg1	measurements					1646:1657	Electrophysiological measurements	1625:1657	Electrophysiological measurements	1625:1657	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	0	32	theme	system	25:30	arg1	Role					0:3	Role	0:3	Role of the melanocortin system in zebrafish skin physiology.	0:60	Role of the melanocortin system in zebrafish skin physiology.
36150411	9	33	theme	severe	1556:1561	arg1	injures					1568:1574	yet severe skin injures	1552:1574	yet severe skin injures	1552:1574	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	3	34	theme	crucial	375:381	arg1	role					383:386	a crucial role	373:386	a crucial role	373:386	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	0	35	theme	zebrafish	35:43	arg1	physiology					50:59	zebrafish skin physiology	35:59	zebrafish skin physiology	35:59	Role of the melanocortin system in zebrafish skin physiology.
36150411	4	36	theme	asip1	734:738	arg1	gene					740:743	the asip1 gene	730:743	the asip1 gene (ASIPzf)	730:752	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	4	36	theme	asip1	734:738	arg1	ASIPzf					746:751	ASIPzf	746:751	ASIPzf	746:751	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	11	37	theme	ASIP1	1813:1817	arg1	animals					1819:1825	ASIP1 animals	1813:1825	ASIP1 animals	1813:1825	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	5	38	theme	wild	910:913	arg1	type					915:918	wild type	910:918	wild type (WT) animals	910:931	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	5	38	theme	wild	910:913	arg1	WT					921:922	WT	921:922	WT	921:922	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	8	39	theme	mucus	1367:1371	arg1	production					1373:1382	an increased mucus production	1354:1382	an increased mucus production	1354:1382	ASIPzf also exhibit higher protein concentration under crowding conditions suggesting an increased mucus production under stressful conditions.
36150411	3	40	theme	chemical	478:485	arg1	barrier					487:493	a chemical barrier	476:493	a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins	476:578	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	0	41	theme	skin	45:48	arg1	physiology					50:59	zebrafish skin physiology	35:59	zebrafish skin physiology	35:59	Role of the melanocortin system in zebrafish skin physiology.
36150411	7	42	theme	lectin	1159:1164	arg1	affinity					1166:1173	lectin affinity	1159:1173	lectin affinity	1159:1173	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	10	43	theme	reduced	1697:1703	arg1	indicator					1731:1739	an indicator	1728:1739	an indicator of reduced tissue integrity and barrier function	1728:1788	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	10	43	theme	reduced	1697:1703	arg1	resistance					1716:1725	reduced epithelial resistance	1697:1725	reduced epithelial resistance	1697:1725	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	7	44	theme	Histochemical	999:1011	arg1	studies					1013:1019	Histochemical studies	999:1019	Histochemical studies	999:1019	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	3	45	theme	due	495:497	arg1	barrier					487:493	a chemical barrier	476:493	a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins	476:578	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	4	46	theme	putative	600:607	arg1	role					609:612	the putative role	596:612	the putative role of ASIP in regulating the immune system of skin	596:660	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	2	47	theme	additional	300:309	arg1	pathways					325:332	additional physiological pathways	300:332	additional physiological pathways	300:332	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	1	48	theme	melanocortin	159:170	arg1	receptors					172:180	melanocortin receptors	159:180	melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish	159:240	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	10	49	theme	tissue	1752:1757	arg1	integrity					1759:1767	reduced tissue integrity	1744:1767	reduced tissue integrity	1744:1767	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	11	50	theme	external	2021:2028	arg1	infections					2030:2039	external infections	2021:2039	external infections	2021:2039	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	3	51	theme	many	550:553	arg1	proteins					571:578	many immune effector proteins	550:578	many immune effector proteins	550:578	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	5	52	theme	structural	770:779	arg1	changes					781:787	the structural changes	766:787	the structural changes in skin induced by asip1 overexpression	766:827	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	6	53	located	found	982:986	arg1	ASIPzf					991:996	ASIPzf	991:996	ASIPzf	991:996	A moderate hypertrophy of mucous cells was also found in ASIPzf.
36150411	6	53	located	found	982:986	arg2	hypertrophy					945:955	A moderate hypertrophy	934:955	A moderate hypertrophy of mucous cells	934:971	A moderate hypertrophy of mucous cells was also found in ASIPzf.
36150411	10	54	theme	function	1781:1788	arg1	indicator					1731:1739	an indicator	1728:1739	an indicator of reduced tissue integrity and barrier function	1728:1788	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	10	54	theme	function	1781:1788	arg1	resistance					1716:1725	reduced epithelial resistance	1697:1725	reduced epithelial resistance	1697:1725	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	5	55	from	changes	781:787	arg1	skin					792:795	skin	792:795	skin induced by asip1 overexpression	792:827	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	9	56	dep	response	1512:1519	arg1	injures					1568:1574	yet severe skin injures	1552:1574	yet severe skin injures	1552:1574	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	1	57	from	patterns	225:232	arg1	fish					237:240	fish	237:240	fish	237:240	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	2	58	theme	potential	256:264	arg1	role					266:269	the potential role	252:269	the potential role of ASIP in the regulation of additional physiological pathways in the skin	252:344	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	2	58	theme	potential	256:264	arg1	unknown					349:355	unknown	349:355	unknown	349:355	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	2	59	from	role	266:269	arg1	regulation					286:295	the regulation	282:295	the regulation of additional physiological pathways in the skin	282:344	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	4	60	theme	skin	657:660	arg1	system					647:652	the immune system	636:652	the immune system of skin	636:660	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	2	61	theme	pathways	325:332	arg1	regulation					286:295	the regulation	282:295	the regulation of additional physiological pathways in the skin	282:344	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	3	62	theme	effector	562:569	arg1	proteins					571:578	many immune effector proteins	550:578	many immune effector proteins	550:578	The skin plays a crucial role in the immune function, acting as a physical limitation against infestation and also as a chemical barrier due to its ability to synthesize and secrete mucus and many immune effector proteins.
36150411	1	63	theme	competitive	113:123	arg1	antagonist					125:134	a competitive antagonist	111:134	a competitive antagonist	111:134	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	1	63	theme	competitive	113:123	arg1	protein					83:89	The agouti-signaling protein	62:89	The agouti-signaling protein (ASIP)	62:96	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	11	64	theme	skin	2001:2004	arg1	sensitivity					2006:2016	increased skin sensitivity	1991:2016	increased skin sensitivity to external infections	1991:2039	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	7	65	theme	cell	1097:1100	arg1	layers					1102:1107	cell layers	1097:1107	cell layers	1097:1107	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	4	66	theme	transgenic	688:697	arg1	model					709:713	a transgenic zebrafish model	686:713	a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf)	686:752	In this study, the putative role of ASIP in regulating the immune system of skin has been explored using a transgenic zebrafish model overexpressing the asip1 gene (ASIPzf).
36150411	2	67	theme	physiological	311:323	arg1	pathways					325:332	additional physiological pathways	300:332	additional physiological pathways	300:332	However, the potential role of ASIP in the regulation of additional physiological pathways in the skin is unknown.
36150411	9	68	theme	pro-inflammatory	1495:1510	arg1	response					1512:1519	a faster pro-inflammatory response	1486:1519	a faster pro-inflammatory response	1486:1519	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	9	69	from	increase	1589:1596	arg1	mortality					1601:1609	mortality	1601:1609	mortality	1601:1609	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	11	70	theme	reduced	1887:1893	arg1	integrity					1911:1919	reduced skin epithelial integrity	1887:1919	reduced skin epithelial integrity	1887:1919	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	5	71	theme	ventral	862:868	arg1	thinner					889:895	thinner	889:895	thinner	889:895	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	5	71	theme	ventral	862:868	arg1	skin					870:873	the ventral skin	858:873	the ventral skin of ASIPzf	858:883	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	6	72	theme	cells	967:971	arg1	hypertrophy					945:955	A moderate hypertrophy	934:955	A moderate hypertrophy of mucous cells	934:971	A moderate hypertrophy of mucous cells was also found in ASIPzf.
36150411	11	73	theme	due	1880:1882	arg1	infections					1869:1878	subsequent infections	1858:1878	subsequent infections due to reduced skin epithelial integrity	1858:1919	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	0	74	theme	melanocortin	12:23	arg1	system					25:30	the melanocortin system	8:30	the melanocortin system	8:30	Role of the melanocortin system in zebrafish skin physiology.
36150411	9	75	theme	slight	1582:1587	arg1	increase					1589:1596	a slight increase	1580:1596	a slight increase in mortality	1580:1609	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	9	76	theme	mucin	1535:1539	arg1	expression					1541:1550	increased mucin expression	1525:1550	increased mucin expression	1525:1550	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	8	77	theme	higher	1288:1293	arg1	concentration					1303:1315	higher protein concentration	1288:1315	higher protein concentration	1288:1315	ASIPzf also exhibit higher protein concentration under crowding conditions suggesting an increased mucus production under stressful conditions.
36150411	1	78	theme	dorsal-ventral	197:210	arg1	patterns					225:232	dorsal-ventral pigmentation patterns	197:232	dorsal-ventral pigmentation patterns in fish	197:240	The agouti-signaling protein (ASIP) acts as both a competitive antagonist and inverse agonist of melanocortin receptors which regulate dorsal-ventral pigmentation patterns in fish.
36150411	6	79	theme	moderate	936:943	arg1	hypertrophy					945:955	A moderate hypertrophy	934:955	A moderate hypertrophy of mucous cells	934:971	A moderate hypertrophy of mucous cells was also found in ASIPzf.
36150411	11	80	theme	epithelial	1900:1909	arg1	integrity					1911:1919	reduced skin epithelial integrity	1887:1919	reduced skin epithelial integrity	1887:1919	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	7	81	theme	transgenic	1033:1042	arg1	animals					1044:1050	transgenic animals	1033:1050	transgenic animals	1033:1050	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	9	82	theme	skin	1563:1566	arg1	injures					1568:1574	yet severe skin injures	1552:1574	yet severe skin injures	1552:1574	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	7	83	theme	mucin	1179:1183	arg1	content					1185:1191	mucin content	1179:1191	mucin content	1179:1191	Histochemical studies showed that transgenic animals appear to compensate for the lower number of cell layers by modifying the mucus composition and increasing lectin affinity and mucin content in order to maintain or improve protection against microorganism adhesion.
36150411	8	84	theme	crowding	1323:1330	arg1	conditions					1332:1341	crowding conditions	1323:1341	crowding conditions suggesting an increased mucus production under stressful conditions	1323:1409	ASIPzf also exhibit higher protein concentration under crowding conditions suggesting an increased mucus production under stressful conditions.
36150411	10	85	theme	ASIP1	1673:1677	arg1	genotype					1679:1686	the ASIP1 genotype	1669:1686	the ASIP1 genotype	1669:1686	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	11	86	theme	subsequent	1858:1867	arg1	infections					1869:1878	subsequent infections	1858:1878	subsequent infections due to reduced skin epithelial integrity	1858:1919	Overall, not only are ASIP1 animals more prone to infiltration and subsequent infections due to reduced skin epithelial integrity, but also display an increased inflammatory response that can lead to increased skin sensitivity to external infections.
36150411	9	87	theme	bacterial	1424:1432	arg1	LPS					1454:1456	LPS	1454:1456	LPS	1454:1456	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	9	87	theme	bacterial	1424:1432	arg1	lipopolysaccharide					1434:1451	bacterial lipopolysaccharide	1424:1451	bacterial lipopolysaccharide (LPS)	1424:1457	Exposure to bacterial lipopolysaccharide (LPS) showed that ASIPzf exhibit a faster pro-inflammatory response and increased mucin expression yet severe skin injures and a slight increase in mortality was observed.
36150411	5	88	theme	type	915:918	arg1	animals					925:931	wild type (WT) animals	910:931	wild type (WT) animals	910:931	Initially, the structural changes in skin induced by asip1 overexpression were studied, revealing that the ventral skin of ASIPzf was thinner than that of wild type (WT) animals.
36150411	8	89	theme	increased	1357:1365	arg1	production					1373:1382	an increased mucus production	1354:1382	an increased mucus production	1354:1382	ASIPzf also exhibit higher protein concentration under crowding conditions suggesting an increased mucus production under stressful conditions.
36150411	10	90	theme	epithelial	1705:1714	arg1	indicator					1731:1739	an indicator	1728:1739	an indicator of reduced tissue integrity and barrier function	1728:1788	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
36150411	10	90	theme	epithelial	1705:1714	arg1	resistance					1716:1725	reduced epithelial resistance	1697:1725	reduced epithelial resistance	1697:1725	Electrophysiological measurements show that the ASIP1 genotype exhibits reduced epithelial resistance, an indicator of reduced tissue integrity and barrier function.
37210613	7	0	theme	betaine	1439:1445	arg1	concentration					1375:1387	the concentration	1371:1387	the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine	1371:1462	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	8	1	theme	enriching	1607:1615	arg1	pathways					1617:1624	enriching pathways	1607:1624	enriching pathways related to the metabolism of bile acids	1607:1664	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	7	2	theme	propionate	1392:1401	arg1	concentration					1375:1387	the concentration	1371:1387	the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine	1371:1462	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	8	3	theme	related	1626:1632	arg1	pathways					1617:1624	enriching pathways	1607:1624	enriching pathways related to the metabolism of bile acids	1607:1664	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	8	4	theme	amino	1677:1681	arg1	acids					1683:1687	amino acids	1677:1687	amino acids	1677:1687	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	9	5	from	upregulation	1784:1795	arg1	metabolites					1847:1857	liver metabolites	1841:1857	liver metabolites	1841:1857	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	6	6	theme	diseases	1107:1114	arg1	DAI					1132:1134	DAI	1132:1134	DAI	1132:1134	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	6	6	theme	diseases	1107:1114	arg1	index					1125:1129	diseases activity index	1107:1129	diseases activity index (DAI)	1107:1135	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	3	7	with	Mice	463:466	arg1	colitis					521:527	dextran sulfate sodium salt (DSS)-induced acute colitis	473:527	dextran sulfate sodium salt (DSS)-induced acute colitis	473:527	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	9	8	from	alterations	1826:1836	arg1	metabolites					1847:1857	liver metabolites	1841:1857	liver metabolites	1841:1857	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	10	9	theme	intestinal	1946:1955	arg1	dysbiosis					1957:1965	intestinal dysbiosis	1946:1965	intestinal dysbiosis	1946:1965	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	3	10	theme	acute	515:519	arg1	colitis					521:527	dextran sulfate sodium salt (DSS)-induced acute colitis	473:527	dextran sulfate sodium salt (DSS)-induced acute colitis	473:527	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	0	11	theme	curcumin	133:140	arg1	effects					122:128	effects	122:128	effects of curcumin	122:140	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	6	12	theme	mucosal	1146:1152	arg1	injury					1154:1159	colonic mucosal injury	1138:1159	colonic mucosal injury	1138:1159	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	4	13	theme	rDNA	647:650	arg1	sequencing					658:667	16S rDNA Miseq sequencing	643:667	16S rDNA Miseq sequencing	643:667	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	10	14	theme	metabolism	1977:1986	arg1	disorders					1988:1996	liver metabolism disorders	1971:1996	liver metabolism disorders	1971:1996	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	4	15	theme	magnetic	685:692	arg1	NMR					709:711	1 H NMR	705:711	1 H NMR	705:711	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	15	theme	magnetic	685:692	arg1	resonance					694:702	proton nuclear magnetic resonance	670:702	proton nuclear magnetic resonance (1 H NMR) spectroscopy	670:725	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	3	16	theme	phosphate	579:587	arg1	PBS					604:606	PBS	604:606	PBS	604:606	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	3	16	theme	phosphate	579:587	arg1	saline					596:601	phosphate buffer saline	579:601	phosphate buffer saline (PBS)	579:607	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	0	17	theme	dysfunction	62:72	arg1	improvement					4:14	The improvement	0:14	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate	0:91	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	3	18	theme	curcumin	567:574	arg1	100 mg/kg					554:562	100 mg/kg	554:562	100 mg/kg of curcumin or phosphate buffer saline (PBS)	554:607	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	7	19	from	concentration	1375:1387	arg1	intestine					1454:1462	the intestine	1450:1462	the intestine	1450:1462	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	1	20	contain	have	175:178	arg1	AIM					158:160	AIM	158:160	AIM	158:160	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	1	20	contain	have	175:178	arg2	application					190:200	promising application	180:200	promising application	180:200	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	1	20	contain	have	175:178	arg1	BACKGROUND					143:152	BACKGROUND	143:152	BACKGROUND	143:152	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	10	21	theme	gut-liver	2045:2053	arg1	axis					2055:2058	the gut-liver axis	2041:2058	the gut-liver axis	2041:2058	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	0	22	theme	dextran	77:83	arg1	sulfate					85:91	dextran sulfate	77:91	dextran sulfate	77:91	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	5	23	theme	correlation	828:838	arg1	SCC					853:855	SCC	853:855	SCC	853:855	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	5	23	theme	correlation	828:838	arg1	coefficient					840:850	Spearman's correlation coefficient	817:850	Spearman's correlation coefficient (SCC)	817:856	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	2	24	dep	gut	367:369	arg1	the					363:365	the	363:365	the	363:365	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	0	25	dep	sodium-induced	93:106	arg1	effects					122:128	effects	122:128	effects of curcumin	122:140	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	8	26	theme	metabolic	1477:1485	arg1	disturbances					1487:1498	hepatic metabolic disturbances	1469:1498	hepatic metabolic disturbances	1469:1498	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	4	27	theme	proton	670:675	arg1	NMR					709:711	1 H NMR	705:711	1 H NMR	705:711	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	27	theme	proton	670:675	arg1	resonance					694:702	proton nuclear magnetic resonance	670:702	proton nuclear magnetic resonance (1 H NMR) spectroscopy	670:725	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	5	28	used	utilized	862:869	arg2	SCC					853:855	SCC	853:855	SCC	853:855	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	5	28	used	utilized	862:869	arg2	coefficient					840:850	Spearman's correlation coefficient	817:850	Spearman's correlation coefficient (SCC)	817:856	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	7	29	theme	microbiota	1249:1258	arg1	composition					1226:1236	the composition	1222:1236	the composition of the gut microbiota	1222:1258	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	1	30	dep	BACKGROUND	143:152	arg1	Curcumin					162:169	Curcumin	162:169	Curcumin	162:169	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	6	31	theme	Curcumin	988:995	arg1	supplementation					997:1011	RESULTS Curcumin supplementation	980:1011	RESULTS Curcumin supplementation	980:1011	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	4	32	theme	liquid	732:737	arg1	LC-MS/MS					780:787	LC-MS/MS	780:787	LC-MS/MS	780:787	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	32	theme	liquid	732:737	arg1	spectrometry					766:777	liquid chromatography-tandem mass spectrometry	732:777	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	732:788	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	0	33	theme	dysbiosis	30:38	arg1	improvement					4:14	The improvement	0:14	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate	0:91	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	5	34	theme	hepatic	949:955	arg1	parameters					968:977	hepatic metabolite parameters	949:977	hepatic metabolite parameters	949:977	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	9	35	theme	probiotics	1811:1820	arg1	alterations					1826:1836	alterations	1826:1836	alterations in liver metabolites	1826:1857	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	9	35	theme	probiotics	1811:1820	arg1	upregulation					1784:1795	the upregulation	1780:1795	the upregulation of intestinal probiotics	1780:1820	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	3	36	theme	saline	596:601	arg1	100 mg/kg					554:562	100 mg/kg	554:562	100 mg/kg of curcumin or phosphate buffer saline (PBS)	554:607	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	4	37	theme	mass	761:764	arg1	LC-MS/MS					780:787	LC-MS/MS	780:787	LC-MS/MS	780:787	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	37	theme	mass	761:764	arg1	spectrometry					766:777	liquid chromatography-tandem mass spectrometry	732:777	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	732:788	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	3	38	theme	METHODS	455:461	arg1	Mice					463:466	METHODS Mice	455:466	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis	455:527	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	0	39	theme	hepatic	44:50	arg1	dysfunction					62:72	hepatic metabolic dysfunction	44:72	hepatic metabolic dysfunction	44:72	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	5	40	theme	parameters	968:977	arg1	modification					909:920	the modification	905:920	the modification of intestinal bacteria and hepatic metabolite parameters	905:977	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	6	41	theme	length	1070:1075	arg1	loss					1040:1043	further loss	1032:1043	further loss of body weight and colon length in IBD mice	1032:1087	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	1	42	dep	prevention	209:218	arg1	the					205:207	the	205:207	the	205:207	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	9	43	theme	liver	1841:1845	arg1	metabolites					1847:1857	liver metabolites	1841:1857	liver metabolites	1841:1857	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	8	44	theme	anthranilic	1557:1567	arg1	acid					1569:1572	anthranilic acid	1557:1572	anthranilic acid	1557:1572	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	6	45	from	loss	1040:1043	arg1	mice					1084:1087	IBD mice	1080:1087	IBD mice	1080:1087	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	1	46	theme	inflammatory	240:251	arg1	IBD					268:270	IBD	268:270	IBD	268:270	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	1	46	theme	inflammatory	240:251	arg1	disease					259:265	inflammatory bowel disease	240:265	inflammatory bowel disease (IBD)	240:271	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	5	47	theme	bacteria	936:943	arg1	modification					909:920	the modification	905:920	the modification of intestinal bacteria and hepatic metabolite parameters	905:977	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	6	48	theme	body	1048:1051	arg1	weight					1053:1058	body weight	1048:1058	body weight	1048:1058	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	10	49	theme	curcumin	1900:1907	arg1	mechanism					1887:1895	The therapeutic mechanism	1871:1895	The therapeutic mechanism of curcumin against IBD mice	1871:1924	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	10	50	theme	IBD	1917:1919	arg1	mice					1921:1924	IBD mice	1917:1924	IBD mice	1917:1924	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	8	51	theme	bile	1655:1658	arg1	acids					1660:1664	bile acids	1655:1664	bile acids	1655:1664	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	1	52	theme	disease	259:265	arg1	amelioration					224:235	amelioration	224:235	amelioration	224:235	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	1	52	theme	disease	259:265	arg1	prevention					209:218	prevention	209:218	prevention	209:218	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	7	53	theme	tryptophan	1423:1432	arg1	concentration					1375:1387	the concentration	1371:1387	the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine	1371:1462	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	7	54	theme	glycine	1414:1420	arg1	concentration					1375:1387	the concentration	1371:1387	the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine	1371:1462	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	6	55	theme	activity	1116:1123	arg1	DAI					1132:1134	DAI	1132:1134	DAI	1132:1134	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	6	55	theme	activity	1116:1123	arg1	index					1125:1129	diseases activity index	1107:1129	diseases activity index (DAI)	1107:1135	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	7	56	theme	butyrate	1404:1411	arg1	concentration					1375:1387	the concentration	1371:1387	the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine	1371:1462	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	2	57	theme	study	448:452	arg1	aim					436:438	the exploration aim	420:438	the exploration aim of this study	420:452	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	2	57	theme	study	448:452	arg1	which					411:415	which	411:415	which	411:415	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	6	58	theme	inflammatory	1166:1177	arg1	infiltration					1179:1190	inflammatory infiltration	1166:1190	inflammatory infiltration	1166:1190	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	1	59	theme	promising	180:188	arg1	application					190:200	promising application	180:200	promising application	180:200	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	4	60	theme	Hematoxylin-eosin	610:626	arg1	staining					633:640	Hematoxylin-eosin (HE) staining	610:640	Hematoxylin-eosin (HE) staining	610:640	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	3	61	theme	-induced	506:513	arg1	colitis					521:527	dextran sulfate sodium salt (DSS)-induced acute colitis	473:527	dextran sulfate sodium salt (DSS)-induced acute colitis	473:527	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	6	62	theme	IBD	1080:1082	arg1	mice					1084:1087	IBD mice	1080:1087	IBD mice	1080:1087	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	6	63	theme	colonic	1138:1144	arg1	injury					1154:1159	colonic mucosal injury	1138:1159	colonic mucosal injury	1138:1159	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	4	64	theme	16S	643:645	arg1	sequencing					658:667	16S rDNA Miseq sequencing	643:667	16S rDNA Miseq sequencing	643:667	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	10	65	theme	liver	1971:1975	arg1	disorders					1988:1996	liver metabolism disorders	1971:1996	liver metabolism disorders	1971:1996	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	4	66	theme	Miseq	652:656	arg1	sequencing					658:667	16S rDNA Miseq sequencing	643:667	16S rDNA Miseq sequencing	643:667	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	9	67	theme	SCC	1726:1728	arg1	analysis					1730:1737	SCC analysis	1726:1737	SCC analysis	1726:1737	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	0	68	from	improvement	4:14	arg1	sulfate					85:91	dextran sulfate	77:91	dextran sulfate	77:91	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	0	69	theme	colitis	108:114	arg1	mice					116:119	colitis mice	108:119	colitis mice	108:119	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	2	70	theme	underlying	287:296	arg1	mechanisms					298:307	the underlying mechanisms	283:307	the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD	283:386	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	8	71	theme	hepatic	1469:1475	arg1	disturbances					1487:1498	hepatic metabolic disturbances	1469:1498	hepatic metabolic disturbances	1469:1498	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	4	72	theme	resonance	694:702	arg1	spectroscopy					714:725	proton nuclear magnetic resonance (1 H NMR) spectroscopy	670:725	proton nuclear magnetic resonance (1 H NMR) spectroscopy	670:725	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	6	73	theme	further	1032:1038	arg1	loss					1040:1043	further loss	1032:1043	further loss of body weight and colon length in IBD mice	1032:1087	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	9	74	theme	potential	1750:1758	arg1	correlation					1760:1770	a potential correlation	1748:1770	a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites	1748:1857	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	7	75	theme	gut	1245:1247	arg1	microbiota					1249:1258	the gut microbiota	1241:1258	the gut microbiota	1241:1258	Meanwhile, curcumin restored the composition of the gut microbiota, significantly increased Akkermansia, Muribaculaceae_unclassified, and Muribaculum, and significantly elevated the concentration of propionate, butyrate, glycine, tryptophan, and betaine in the intestine.
37210613	8	76	theme	curcumin	1501:1508	arg1	intervention					1510:1521	curcumin intervention	1501:1521	curcumin intervention	1501:1521	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	2	77	dep	ability	326:332	arg1	interact					349:356	interact	349:356	to interact with the gut and liver in IBD	346:386	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	0	78	theme	intestinal	19:28	arg1	dysbiosis					30:38	intestinal dysbiosis	19:38	intestinal dysbiosis	19:38	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	4	79	theme	H	707:707	arg1	NMR					709:711	1 H NMR	705:711	1 H NMR	705:711	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	79	theme	H	707:707	arg1	resonance					694:702	proton nuclear magnetic resonance	670:702	proton nuclear magnetic resonance (1 H NMR) spectroscopy	670:725	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	80	theme	nuclear	677:683	arg1	NMR					709:711	1 H NMR	705:711	1 H NMR	705:711	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	80	theme	nuclear	677:683	arg1	resonance					694:702	proton nuclear magnetic resonance	670:702	proton nuclear magnetic resonance (1 H NMR) spectroscopy	670:725	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	6	81	theme	RESULTS	980:986	arg1	supplementation					997:1011	RESULTS Curcumin supplementation	980:1011	RESULTS Curcumin supplementation	980:1011	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	9	82	theme	intestinal	1800:1809	arg1	probiotics					1811:1820	intestinal probiotics	1800:1820	intestinal probiotics	1800:1820	Furthermore, SCC analysis revealed a potential correlation between the upregulation of intestinal probiotics and alterations in liver metabolites.
37210613	3	83	theme	buffer	589:594	arg1	PBS					604:606	PBS	604:606	PBS	604:606	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	3	83	theme	buffer	589:594	arg1	saline					596:601	phosphate buffer saline	579:601	phosphate buffer saline (PBS)	579:607	METHODS Mice with dextran sulfate sodium salt (DSS)-induced acute colitis were treated either with 100 mg/kg of curcumin or phosphate buffer saline (PBS).
37210613	0	84	theme	metabolic	52:60	arg1	dysfunction					62:72	hepatic metabolic dysfunction	44:72	hepatic metabolic dysfunction	44:72	The improvement of intestinal dysbiosis and hepatic metabolic dysfunction in dextran sulfate sodium-induced colitis mice: effects of curcumin.
37210613	5	85	theme	metabolite	957:966	arg1	parameters					968:977	hepatic metabolite parameters	949:977	hepatic metabolite parameters	949:977	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	2	86	theme	exploration	424:434	arg1	aim					436:438	the exploration aim	420:438	the exploration aim of this study	420:452	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	2	86	theme	exploration	424:434	arg1	which					411:415	which	411:415	which	411:415	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	10	87	theme	therapeutic	1875:1885	arg1	mechanism					1887:1895	The therapeutic mechanism	1871:1895	The therapeutic mechanism of curcumin against IBD mice	1871:1924	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	4	88	theme	chromatography-tandem	739:759	arg1	LC-MS/MS					780:787	LC-MS/MS	780:787	LC-MS/MS	780:787	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	4	88	theme	chromatography-tandem	739:759	arg1	spectrometry					766:777	liquid chromatography-tandem mass spectrometry	732:777	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	732:788	Hematoxylin-eosin (HE) staining, 16S rDNA Miseq sequencing, proton nuclear magnetic resonance (1 H NMR) spectroscopy, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were applied for analysis.
37210613	2	89	theme	curcumin	337:344	arg1	ability					326:332	the ability	322:332	the ability of curcumin to interact with the gut and liver in IBD	322:386	However, the underlying mechanisms underpinning the ability of curcumin to interact with the gut and liver in IBD remains to be defined, which is the exploration aim of this study.
37210613	6	90	theme	colon	1064:1068	arg1	length					1070:1075	colon length	1064:1075	colon length	1064:1075	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	10	91	dep	CONCLUSION	1860:1869	arg1	occurs					1926:1931	occurs	1926:1931	occurs	1926:1931	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	5	92	theme	intestinal	925:934	arg1	bacteria					936:943	intestinal bacteria	925:943	intestinal bacteria	925:943	Spearman's correlation coefficient (SCC) was utilized to assess the correlation between the modification of intestinal bacteria and hepatic metabolite parameters.
37210613	6	93	theme	weight	1053:1058	arg1	loss					1040:1043	further loss	1032:1043	further loss of body weight and colon length in IBD mice	1032:1087	RESULTS Curcumin supplementation not only prevented further loss of body weight and colon length in IBD mice but also improved diseases activity index (DAI), colonic mucosal injury, and inflammatory infiltration.
37210613	10	94	theme	axis	2055:2058	arg1	stabilization					2024:2036	the stabilization	2020:2036	the stabilization of the gut-liver axis	2020:2058	CONCLUSION The therapeutic mechanism of curcumin against IBD mice occurs by improving intestinal dysbiosis and liver metabolism disorders, thus contributing to the stabilization of the gut-liver axis.
37210613	8	95	theme	acids	1660:1664	arg1	metabolism					1641:1650	the metabolism	1637:1650	the metabolism of bile acids	1637:1664	For hepatic metabolic disturbances, curcumin intervention altered 14 metabolites, including anthranilic acid and 8-amino-7-oxononanoate while enriching pathways related to the metabolism of bile acids, glucagon, amino acids, biotin, and butanoate.
37210613	1	96	theme	bowel	253:257	arg1	IBD					268:270	IBD	268:270	IBD	268:270	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
37210613	1	96	theme	bowel	253:257	arg1	disease					259:265	inflammatory bowel disease	240:265	inflammatory bowel disease (IBD)	240:271	BACKGROUND AND AIM Curcumin may have promising application in the prevention and amelioration of inflammatory bowel disease (IBD).
36735684	6	0	theme	greater	1309:1315	arg1	proportion					1317:1326	a greater proportion	1307:1326	a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells	1307:1380	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	11	1	theme	long-lasting	2483:2494	arg1	effect					2496:2501	long-lasting effect	2483:2501	long-lasting effect	2483:2501	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	7	2	dep	decreased	1574:1582	arg1	whereas					1704:1710	whereas	1704:1710	whereas	1704:1710	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	9	3	theme	line	2113:2116	arg1	differences					2118:2128	line differences	2113:2128	line differences	2113:2128	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	7	4	theme	heat	1589:1592	arg1	43°C					1602:1605	43°C	1602:1605	43°C	1602:1605	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	7	4	theme	heat	1589:1592	arg1	stress					1594:1599	heat stress	1589:1599	heat stress (43°C)	1589:1606	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	8	5	theme	thermal	1909:1915	arg1	stress					1917:1922	both hot and cold thermal stress	1891:1922	both hot and cold thermal stress	1891:1922	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	9	6	theme	cells	2069:2073	arg1	proportion					2044:2053	the proportion	2040:2053	the proportion of SDC4-CD44+ cells	2040:2073	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	10	7	theme	growth-selected	2229:2243	arg1	turkey					2256:2261	growth-selected commercial turkey	2229:2261	growth-selected commercial turkey	2229:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	6	8	theme	cells	1297:1301	arg1	proportion					1238:1247	a lower proportion	1230:1247	a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells	1230:1301	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	7	9	theme	heat	1762:1765	arg1	stress					1767:1772	heat stress	1762:1772	heat stress	1762:1772	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	6	10	contain	contain	1222:1228	arg1	SCs					1204:1206	The NC line SCs	1192:1206	The NC line SCs	1192:1206	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	6	10	contain	contain	1222:1228	arg2	proportion					1238:1247	a lower proportion	1230:1247	a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells	1230:1301	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	10	11	theme	SCs	2222:2224	arg1	composition					2191:2201	differential composition	2178:2201	differential composition of p. major muscle SCs in growth-selected commercial turkey	2178:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	3	12	theme	Pivotal	555:561	arg1	genes					563:567	Pivotal genes	555:567	Pivotal genes in mTOR and Wnt/PCP pathways	555:596	Pivotal genes in mTOR and Wnt/PCP pathways are mTOR and frizzled-7 (Fzd7), respectively.
36735684	3	12	theme	Pivotal	555:561	arg1	mTOR					602:605	mTOR	602:605	mTOR	602:605	Pivotal genes in mTOR and Wnt/PCP pathways are mTOR and frizzled-7 (Fzd7), respectively.
36735684	4	13	theme	mTOR	850:853	arg1	pathways					867:874	the mTOR and Wnt/PCP pathways	846:874	the mTOR and Wnt/PCP pathways	846:874	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	7	14	theme	SDC4+CD44-	1557:1566	arg1	cells					1568:1572	SDC4+CD44- cells	1557:1572	SDC4+CD44- cells	1557:1572	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	5	15	theme	slower-growing	996:1009	arg1	turkeys					1053:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	5	16	theme	differentiation	1175:1189	arg1	h					1170:1170	48 h	1167:1170	48 h of differentiation	1167:1189	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	7	17	theme	SDC4-CD44+	1730:1739	arg1	cells					1741:1745	SDC4-CD44+ cells	1730:1745	SDC4-CD44+ cells	1730:1745	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	11	18	theme	SC	2450:2451	arg1	populations					2453:2463	growth-selected SC populations	2434:2463	growth-selected SC populations	2434:2463	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	4	19	theme	Wnt/PCP	859:865	arg1	pathways					867:874	the mTOR and Wnt/PCP pathways	846:874	the mTOR and Wnt/PCP pathways	846:874	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	11	20	theme	muscle	2506:2511	arg1	development					2513:2523	muscle development	2506:2523	muscle development	2506:2523	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	0	21	theme	turkey	136:141	arg1	populations					158:168	growth-selected turkey satellite cell populations	120:168	growth-selected turkey satellite cell populations	120:168	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	5	22	theme	Randombred	1020:1029	arg1	turkeys					1053:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	11	23	theme	growth-selected	2434:2448	arg1	populations					2453:2463	growth-selected SC populations	2434:2463	growth-selected SC populations	2434:2463	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	0	24	theme	cell	153:156	arg1	populations					158:168	growth-selected turkey satellite cell populations	120:168	growth-selected turkey satellite cell populations	120:168	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	5	25	theme	NC	980:981	arg1	turkeys					984:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	5	26	theme	turkeys	984:990	arg1	muscle					925:930	the p. major muscle	912:930	the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys	912:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	7	27	theme	proliferation	1524:1536	arg1	h					1519:1519	72 h	1516:1519	72 h of proliferation	1516:1536	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	2	28	theme	tumor	478:482	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	28	theme	tumor	478:482	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	0	29	theme	cell	60:63	arg1	pathways					74:81	Wnt-planar cell polarity pathways	49:81	Wnt-planar cell polarity pathways	49:81	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	2	30	theme	mouse	464:468	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	30	theme	mouse	464:468	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	11	31	theme	populations	2453:2463	arg1	sensitivity					2380:2390	differential temperature sensitivity	2355:2390	differential temperature sensitivity	2355:2390	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	11	31	theme	populations	2453:2463	arg1	responses					2421:2429	Wnt/PCP pathway responses	2405:2429	Wnt/PCP pathway responses of growth-selected SC populations	2405:2463	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	11	31	theme	populations	2453:2463	arg1	mTOR					2396:2399	mTOR	2396:2399	mTOR	2396:2399	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	0	32	theme	pathways	74:81	arg1	effects					13:19	Differential effects	0:19	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.	0:169	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	4	33	from	SDC4	735:738	arg1	turkey					763:768	turkey	763:768	turkey	763:768	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	1	34	theme	Satellite	171:179	arg1	SCs					188:190	SCs	188:190	SCs	188:190	Satellite cells (SCs) comprise a heterogeneous population of muscle stem cells.
36735684	1	34	theme	Satellite	171:179	arg1	cells					181:185	Satellite cells	171:185	Satellite cells (SCs)	171:191	Satellite cells (SCs) comprise a heterogeneous population of muscle stem cells.
36735684	2	35	theme	family/planar	507:519	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	35	theme	family/planar	507:519	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	4	36	theme	stress	725:730	arg1	effects					706:712	the differential effects	689:712	the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways	689:874	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	0	37	theme	syndecan-4	86:95	arg1	expression					106:115	syndecan-4 and CD44 expression	86:115	expression	106:115	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	5	38	theme	Line	1039:1042	arg1	turkeys					1053:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	2	39	theme	integration	490:500	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	39	theme	integration	490:500	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	10	40	from	composition	2191:2201	arg1	turkey					2256:2261	growth-selected commercial turkey	2229:2261	growth-selected commercial turkey	2229:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	9	41	theme	SDC4+CD44-	2017:2026	arg1	cells					2028:2032	SDC4+CD44- cells	2017:2032	SDC4+CD44- cells	2017:2032	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	9	42	theme	expression	1978:1987	arg1	Knockdown					1952:1960	Knockdown	1952:1960	Knockdown of mTOR or Fzd7 expression	1952:1987	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	0	43	theme	CD44	101:104	arg1	expression					106:115	syndecan-4 and CD44 expression	86:115	expression	106:115	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	2	44	theme	rapamycin	429:437	arg1	target					419:424	mechanistic target	407:424	mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways	407:552	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	6	45	theme	positive	1257:1264	arg1	proportion					1238:1247	a lower proportion	1230:1247	a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells	1230:1301	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	4	46	theme	differential	693:704	arg1	effects					706:712	the differential effects	689:712	the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways	689:874	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	2	47	theme	major	379:383	arg1	muscle					396:401	turkey pectoralis major (p. major) muscle	361:401	turkey pectoralis major (p. major) muscle	361:401	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	0	48	theme	Differential	0:11	arg1	effects					13:19	Differential effects	0:19	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.	0:169	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	2	49	theme	Thermal	251:257	arg1	stress					259:264	Thermal stress	251:264	Thermal stress during the first week after hatch	251:298	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	6	50	theme	SDC4+CD44-	1285:1294	arg1	cells					1297:1301	SDC4 positive and CD44 negative (SDC4+CD44-) cells	1252:1301	cells	1297:1301	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	8	51	theme	SDC4	1836:1839	arg1	expression					1822:1831	the expression	1818:1831	the expression of SDC4 and CD44 in the NC SCs	1818:1862	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	10	52	theme	major	2209:2213	arg1	SCs					2222:2224	p. major muscle SCs	2206:2224	p. major muscle SCs in growth-selected commercial turkey	2206:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	11	53	theme	temperature	2368:2378	arg1	sensitivity					2380:2390	differential temperature sensitivity	2355:2390	differential temperature sensitivity	2355:2390	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	4	54	theme	p.	770:771	arg1	SCs					786:788	p. major muscle SCs	770:788	p. major muscle SCs	770:788	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	9	55	theme	cells	2028:2032	arg1	proportion					2003:2012	the proportion	1999:2012	the proportion of SDC4+CD44- cells	1999:2032	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	6	56	theme	negative	1275:1282	arg1	cells					1297:1301	SDC4 positive and CD44 negative (SDC4+CD44-) cells	1252:1301	cells	1297:1301	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	7	57	theme	control	1662:1668	arg1	38°C					1683:1686	38°C	1683:1686	38°C	1683:1686	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	7	57	theme	control	1662:1668	arg1	temperature					1670:1680	the control temperature	1658:1680	the control temperature (38°C)	1658:1687	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	5	58	theme	faster-growing	946:959	arg1	turkeys					984:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	8	59	theme	CD44	1845:1848	arg1	expression					1822:1831	the expression	1818:1831	the expression of SDC4 and CD44 in the NC SCs	1818:1862	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	11	60	theme	pathway	2413:2419	arg1	responses					2421:2429	Wnt/PCP pathway responses	2405:2429	Wnt/PCP pathway responses of growth-selected SC populations	2405:2463	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	4	61	theme	muscle	779:784	arg1	SCs					786:788	p. major muscle SCs	770:788	p. major muscle SCs	770:788	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	4	62	theme	CD44	744:747	arg1	expression					749:758	CD44 expression	744:758	CD44 expression in turkey	744:768	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	5	63	theme	thermal	1114:1120	arg1	stress					1122:1127	hot (43°C) and cold (33°C) thermal stress	1087:1127	stress	1122:1127	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	5	64	theme	major	919:923	arg1	muscle					925:930	the p. major muscle	912:930	the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys	912:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	2	65	theme	turkey	361:366	arg1	muscle					396:401	turkey pectoralis major (p. major) muscle	361:401	turkey pectoralis major (p. major) muscle	361:401	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	1	66	theme	stem	239:242	arg1	cells					244:248	muscle stem cells	232:248	muscle stem cells	232:248	Satellite cells (SCs) comprise a heterogeneous population of muscle stem cells.
36735684	6	67	theme	CD44	1270:1273	arg1	cells					1297:1301	SDC4 positive and CD44 negative (SDC4+CD44-) cells	1252:1301	cells	1297:1301	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	8	68	theme	greater	1871:1877	arg1	response					1879:1886	greater response	1871:1886	greater response to both hot and cold thermal stress compared to the RBC2 cells	1871:1949	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	2	69	theme	SCs	354:356	arg1	myogenesis					322:331	myogenesis	322:331	myogenesis	322:331	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	69	theme	SCs	354:356	arg1	adipogenesis					338:349	adipogenesis	338:349	adipogenesis	338:349	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	69	theme	SCs	354:356	arg1	proliferation					307:319	proliferation	307:319	proliferation	307:319	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	5	70	theme	cold	1102:1105	arg1	stress					1122:1127	hot (43°C) and cold (33°C) thermal stress	1087:1127	stress	1122:1127	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	5	71	theme	33°C	1108:1111	arg1	stress					1122:1127	hot (43°C) and cold (33°C) thermal stress	1087:1127	stress	1122:1127	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	7	72	theme	cold	1793:1796	arg1	stress					1798:1803	cold stress	1793:1803	cold stress	1793:1803	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	4	73	theme	SDC4	816:819	arg1	expression					802:811	the expression	798:811	the expression of SDC4 and CD44	798:828	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	8	74	theme	hot	1896:1898	arg1	stress					1917:1922	both hot and cold thermal stress	1891:1922	both hot and cold thermal stress	1891:1922	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	2	75	theme	first	277:281	arg1	week					283:286	the first week	273:286	the first week after hatch	273:298	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	6	76	theme	differentiation	1484:1498	arg1	h					1479:1479	48 h	1476:1479	48 h of differentiation	1476:1498	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	6	76	theme	differentiation	1484:1498	arg1	h					1453:1453	72 h	1450:1453	72 h of proliferation	1450:1470	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	11	77	contain	have	2478:2481	arg1	this					2469:2472	this	2469:2472	this	2469:2472	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	11	77	contain	have	2478:2481	arg2	effect					2496:2501	long-lasting effect	2483:2501	long-lasting effect	2483:2501	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	7	78	theme	cells	1568:1572	arg1	proportion					1543:1552	the proportion	1539:1552	the proportion of SDC4+CD44- cells	1539:1572	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	8	79	theme	cold	1904:1907	arg1	stress					1917:1922	both hot and cold thermal stress	1891:1922	both hot and cold thermal stress	1891:1922	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	6	80	theme	cells	1376:1380	arg1	proportion					1317:1326	a greater proportion	1307:1326	a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells	1307:1380	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	5	81	attach	isolated	898:905	arg1	muscle					925:930	the p. major muscle	912:930	the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys	912:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	5	81	attach	isolated	898:905	arg2	cells					887:891	Satellite cells	877:891	Satellite cells	877:891	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	10	82	theme	muscle	2215:2220	arg1	SCs					2222:2224	p. major muscle SCs	2206:2224	p. major muscle SCs in growth-selected commercial turkey	2206:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	0	83	theme	growth-selected	120:134	arg1	populations					158:168	growth-selected turkey satellite cell populations	120:168	growth-selected turkey satellite cell populations	120:168	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	9	84	theme	treatment	2148:2156	arg1	temperatures					2158:2169	treatment temperatures	2148:2169	treatment temperatures	2148:2169	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	6	85	theme	SDC4-CD44+	1364:1373	arg1	cells					1376:1380	SDC4 negative and CD44 positive (SDC4-CD44+) cells	1331:1380	cells	1376:1380	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	0	86	theme	satellite	143:151	arg1	populations					158:168	growth-selected turkey satellite cell populations	120:168	growth-selected turkey satellite cell populations	120:168	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	5	87	theme	historic	1011:1018	arg1	turkeys					1053:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	3	88	theme	mTOR	572:575	arg1	pathways					589:596	mTOR and Wnt/PCP pathways	572:596	mTOR and Wnt/PCP pathways	572:596	Pivotal genes in mTOR and Wnt/PCP pathways are mTOR and frizzled-7 (Fzd7), respectively.
36735684	6	89	theme	positive	1354:1361	arg1	cells					1376:1380	SDC4 negative and CD44 positive (SDC4-CD44+) cells	1331:1380	cells	1376:1380	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	1	90	theme	heterogeneous	204:216	arg1	population					218:227	a heterogeneous population	202:227	a heterogeneous population of muscle stem cells	202:248	Satellite cells (SCs) comprise a heterogeneous population of muscle stem cells.
36735684	7	91	theme	cells	1741:1745	arg1	proportion					1716:1725	the proportion	1712:1725	the proportion of SDC4-CD44+ cells	1712:1745	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	2	92	theme	cell	521:524	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	92	theme	cell	521:524	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	8	93	theme	RBC2	1940:1943	arg1	cells					1945:1949	the RBC2 cells	1936:1949	the RBC2 cells	1936:1949	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	10	94	from	SCs	2222:2224	arg1	turkey					2256:2261	growth-selected commercial turkey	2229:2261	growth-selected commercial turkey	2229:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	5	95	dep	major	919:923	arg1	p.					916:917	p.	916:917	p.	916:917	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	6	96	theme	NC	1196:1197	arg1	SCs					1204:1206	The NC line SCs	1192:1206	The NC line SCs	1192:1206	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	5	97	theme	Control	1031:1037	arg1	turkeys					1053:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	4	98	theme	CD44	825:828	arg1	expression					802:811	the expression	798:811	the expression of SDC4 and CD44	798:828	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	0	99	from	expression	106:115	arg1	populations					158:168	growth-selected turkey satellite cell populations	120:168	growth-selected turkey satellite cell populations	120:168	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	4	100	theme	study	666:670	arg1	objective					648:656	The objective	644:656	The objective of this study	644:670	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	6	101	theme	control	1415:1421	arg1	temperature					1423:1433	the control temperature	1411:1433	the control temperature (38°C)	1411:1440	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	6	101	theme	control	1415:1421	arg1	38°C					1436:1439	38°C	1436:1439	38°C	1436:1439	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	10	102	theme	CD44	2321:2324	arg1	expression					2326:2335	CD44 expression	2321:2335	CD44 expression	2321:2335	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	10	103	from	turkey	2256:2261	arg1	composition					2191:2201	differential composition	2178:2201	differential composition of p. major muscle SCs in growth-selected commercial turkey	2178:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	0	104	theme	polarity	65:72	arg1	pathways					74:81	Wnt-planar cell polarity pathways	49:81	Wnt-planar cell polarity pathways	49:81	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	3	105	theme	Wnt/PCP	581:587	arg1	pathways					589:596	mTOR and Wnt/PCP pathways	572:596	mTOR and Wnt/PCP pathways	572:596	Pivotal genes in mTOR and Wnt/PCP pathways are mTOR and frizzled-7 (Fzd7), respectively.
36735684	4	106	from	expression	749:758	arg1	turkey					763:768	turkey	763:768	turkey	763:768	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	4	107	dep	SDC4	735:738	arg1	SCs					786:788	p. major muscle SCs	770:788	p. major muscle SCs	770:788	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	2	108	theme	mammary	470:476	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	108	theme	mammary	470:476	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	5	109	theme	turkeys	1053:1059	arg1	muscle					925:930	the p. major muscle	912:930	the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys	912:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	6	110	theme	RBC2	1398:1401	arg1	line					1403:1406	the RBC2 line	1394:1406	the RBC2 line	1394:1406	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	2	111	theme	wingless-type	450:462	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	111	theme	wingless-type	450:462	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	0	112	from	effects	13:19	arg1	expression					106:115	syndecan-4 and CD44 expression	86:115	expression	106:115	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	5	113	theme	proliferation	1141:1153	arg1	h					1136:1136	72 h	1133:1136	72 h of proliferation followed by 48 h of differentiation	1133:1189	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	9	114	theme	mTOR	1965:1968	arg1	Knockdown					1952:1960	Knockdown	1952:1960	Knockdown of mTOR or Fzd7 expression	1952:1987	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	6	115	theme	lower	1232:1236	arg1	proportion					1238:1247	a lower proportion	1230:1247	a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells	1230:1301	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	6	116	theme	proliferation	1458:1470	arg1	h					1479:1479	48 h	1476:1479	48 h of differentiation	1476:1498	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	6	116	theme	proliferation	1458:1470	arg1	h					1453:1453	72 h	1450:1453	72 h of proliferation	1450:1470	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	2	117	theme	site	502:505	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	117	theme	site	502:505	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	4	118	theme	thermal	717:723	arg1	stress					725:730	thermal stress	717:730	thermal stress	717:730	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	9	119	theme	Fzd7	1973:1976	arg1	expression					1978:1987	Fzd7 expression	1973:1987	Fzd7 expression	1973:1987	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	2	120	theme	virus	484:488	arg1	Wnt/PCP					536:542	Wnt/PCP	536:542	Wnt/PCP	536:542	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	2	120	theme	virus	484:488	arg1	polarity					526:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity	450:533	wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP)	450:543	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	5	121	theme	RBC2	1047:1050	arg1	turkeys					1053:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	slower-growing historic Randombred Control Line 2 (RBC2) turkeys	996:1059	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	2	122	dep	rapamycin	429:437	arg1	pathways					545:552	pathways	545:552	pathways	545:552	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	4	123	from	effects	706:712	arg1	expression					749:758	CD44 expression	744:758	CD44 expression in turkey	744:768	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	4	123	from	effects	706:712	arg1	modulated					833:841	modulated	833:841	is modulated by the mTOR and Wnt/PCP pathways	830:874	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	4	123	from	effects	706:712	arg1	SDC4					735:738	SDC4	735:738	SDC4	735:738	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	2	124	theme	polarity	526:533	arg1	target					419:424	mechanistic target	407:424	mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways	407:552	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	10	125	from	alteration	2298:2307	arg1	SDC4					2312:2315	SDC4	2312:2315	SDC4	2312:2315	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	10	125	from	alteration	2298:2307	arg1	expression					2326:2335	CD44 expression	2321:2335	CD44 expression	2321:2335	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	3	126	from	genes	563:567	arg1	pathways					589:596	mTOR and Wnt/PCP pathways	572:596	mTOR and Wnt/PCP pathways	572:596	Pivotal genes in mTOR and Wnt/PCP pathways are mTOR and frizzled-7 (Fzd7), respectively.
36735684	2	127	theme	pectoralis	368:377	arg1	muscle					396:401	turkey pectoralis major (p. major) muscle	361:401	turkey pectoralis major (p. major) muscle	361:401	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	0	128	theme	temperature	24:34	arg1	effects					13:19	Differential effects	0:19	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.	0:169	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	5	129	theme	Satellite	877:885	arg1	cells					887:891	Satellite cells	877:891	Satellite cells	877:891	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	2	130	theme	mechanistic	407:417	arg1	target					419:424	mechanistic target	407:424	mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways	407:552	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	11	131	theme	differential	2355:2366	arg1	sensitivity					2380:2390	differential temperature sensitivity	2355:2390	differential temperature sensitivity	2355:2390	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	2	132	theme	muscle	396:401	arg1	SCs					354:356	SCs	354:356	SCs of turkey pectoralis major (p. major) muscle	354:401	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	0	133	theme	mTOR	40:43	arg1	effects					13:19	Differential effects	0:19	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.	0:169	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	5	134	theme	1-week-old	935:944	arg1	turkeys					984:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	10	135	theme	p.	2206:2207	arg1	SCs					2222:2224	p. major muscle SCs	2206:2224	p. major muscle SCs in growth-selected commercial turkey	2206:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	11	136	theme	Wnt/PCP	2405:2411	arg1	responses					2421:2429	Wnt/PCP pathway responses	2405:2429	Wnt/PCP pathway responses of growth-selected SC populations	2405:2463	Results indicate differential temperature sensitivity and mTOR and Wnt/PCP pathway responses of growth-selected SC populations and this may have long-lasting effect on muscle development and growth.
36735684	2	137	dep	major	389:393	arg1	p.					386:387	p.	386:387	p.	386:387	Thermal stress during the first week after hatch alters proliferation, myogenesis, and adipogenesis of SCs of turkey pectoralis major (p. major) muscle via mechanistic target of rapamycin (mTOR) and wingless-type mouse mammary tumor virus integration site family/planar cell polarity (Wnt/PCP) pathways.
36735684	0	138	theme	Wnt-planar	49:58	arg1	pathways					74:81	Wnt-planar cell polarity pathways	49:81	Wnt-planar cell polarity pathways	49:81	Differential effects of temperature and mTOR and Wnt-planar cell polarity pathways on syndecan-4 and CD44 expression in growth-selected turkey satellite cell populations.
36735684	7	139	theme	relative	1646:1653	arg1	33°C					1640:1643	33°C	1640:1643	33°C	1640:1643	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	7	139	theme	relative	1646:1653	arg1	stress					1632:1637	cold stress	1627:1637	cold stress (33°C) relative to the control temperature (38°C)	1627:1687	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	5	140	theme	modern-commercial	961:977	arg1	turkeys					984:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	1-week-old faster-growing modern-commercial (NC) turkeys	935:990	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	8	141	from	expression	1822:1831	arg1	SCs					1860:1862	the NC SCs	1853:1862	the NC SCs	1853:1862	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	8	142	theme	NC	1857:1858	arg1	SCs					1860:1862	the NC SCs	1853:1862	the NC SCs	1853:1862	In general, the expression of SDC4 and CD44 in the NC SCs showed greater response to both hot and cold thermal stress compared to the RBC2 cells.
36735684	6	143	theme	line	1199:1202	arg1	SCs					1204:1206	The NC line SCs	1192:1206	The NC line SCs	1192:1206	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	4	144	theme	major	773:777	arg1	SCs					786:788	p. major muscle SCs	770:788	p. major muscle SCs	770:788	The objective of this study was to determine the differential effects of thermal stress on SDC4 and CD44 expression in turkey p. major muscle SCs and how the expression of SDC4 and CD44 is modulated by the mTOR and Wnt/PCP pathways.
36735684	6	145	theme	CD44	1349:1352	arg1	cells					1376:1380	SDC4 negative and CD44 positive (SDC4-CD44+) cells	1331:1380	cells	1376:1380	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	5	146	theme	hot	1087:1089	arg1	43°C					1092:1095	hot (43°C) and cold (33°C) thermal stress	1087:1127	43°C	1092:1095	Satellite cells were isolated from the p. major muscle of 1-week-old faster-growing modern-commercial (NC) turkeys and slower-growing historic Randombred Control Line 2 (RBC2) turkeys, and were challenged with hot (43°C) and cold (33°C) thermal stress for 72 h of proliferation followed by 48 h of differentiation.
36735684	7	147	theme	cold	1627:1630	arg1	33°C					1640:1643	33°C	1640:1643	33°C	1640:1643	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	7	147	theme	cold	1627:1630	arg1	stress					1632:1637	cold stress	1627:1637	cold stress (33°C) relative to the control temperature (38°C)	1627:1687	In general, at 72 h of proliferation, the proportion of SDC4+CD44- cells decreased with heat stress (43°C) and increased with cold stress (33°C) relative to the control temperature (38°C) in both lines, whereas the proportion of SDC4-CD44+ cells increased with heat stress and decreased with cold stress.
36735684	9	148	theme	SDC4-CD44+	2058:2067	arg1	cells					2069:2073	SDC4-CD44+ cells	2058:2073	SDC4-CD44+ cells	2058:2073	Knockdown of mTOR or Fzd7 expression increased the proportion of SDC4+CD44- cells while the proportion of SDC4-CD44+ cells decreased during differentiation with line differences being specific to treatment temperatures.
36735684	6	149	theme	negative	1336:1343	arg1	proportion					1317:1326	a greater proportion	1307:1326	a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells	1307:1380	The NC line SCs were found to contain a lower proportion of SDC4 positive and CD44 negative (SDC4+CD44-) cells and a greater proportion of SDC4 negative and CD44 positive (SDC4-CD44+) cells compared to the RBC2 line at the control temperature (38°C) at both 72 h of proliferation and 48 h of differentiation.
36735684	1	150	theme	muscle	232:237	arg1	cells					244:248	muscle stem cells	232:248	muscle stem cells	232:248	Satellite cells (SCs) comprise a heterogeneous population of muscle stem cells.
36735684	10	151	theme	commercial	2245:2254	arg1	turkey					2256:2261	growth-selected commercial turkey	2229:2261	growth-selected commercial turkey	2229:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	10	152	theme	differential	2178:2189	arg1	composition					2191:2201	differential composition	2178:2201	differential composition of p. major muscle SCs in growth-selected commercial turkey	2178:2261	Thus, differential composition of p. major muscle SCs in growth-selected commercial turkey may be resulted, in part, from the alteration in SDC4 and CD44 expression.
36735684	1	153	theme	cells	244:248	arg1	population					218:227	a heterogeneous population	202:227	a heterogeneous population of muscle stem cells	202:248	Satellite cells (SCs) comprise a heterogeneous population of muscle stem cells.
36256564	9	0	theme	digital	1105:1111	arg1	values					1133:1138	The digital DNA-DNA relatedness values	1101:1138	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T	1101:1189	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	9	0	theme	digital	1105:1111	arg1	%					1200:1200	21.6 %	1195:1200	21.6 %	1195:1200	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	14	1	theme	described	1606:1614	arg1	characteristics					1663:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	2	2	theme	Gram-stain-positive	138:156	arg1	strain					180:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	5	3	theme	highest	609:615	arg1	similarity					626:635	the highest pairwise similarity	605:635	the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T	605:685	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	9	4	theme	relatedness	1121:1131	arg1	values					1133:1138	The digital DNA-DNA relatedness values	1101:1138	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T	1101:1189	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	9	4	theme	relatedness	1121:1131	arg1	%					1200:1200	21.6 %	1195:1200	21.6 %	1195:1200	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	11	5	theme	fatty	1374:1378	arg1	acids					1380:1384	The predominant cellular fatty acids	1349:1384	The predominant cellular fatty acids	1349:1384	The predominant cellular fatty acids were anteiso-C15 : 0 (75 %) and anteiso-C17 : 0 (15.2 %).
36256564	2	6	theme	aerobic	129:135	arg1	strain					180:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	5	7	theme	gene	575:578	arg1	sequence					580:587	The 16S rRNA gene sequence	562:587	The 16S rRNA gene sequence of C1-1T	562:596	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	6	8	theme	whole-genome	733:744	arg1	sequences					746:754	whole-genome sequences	733:754	whole-genome sequences	733:754	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	2	9	theme	frost-sorted	263:274	arg1	polygons					276:283	frost-sorted polygons	263:283	frost-sorted polygons	263:283	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	6	10	theme	psychrolactophilus	877:894	arg1	group					896:900	the 'Arthrobacter psychrolactophilus group'	859:901	the 'Arthrobacter psychrolactophilus group'	859:901	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	14	11	theme	Arthrobacter	1772:1783	arg1	sp					1793:1794	a novel species Arthrobacter polaris sp	1756:1794	a novel species Arthrobacter polaris sp	1756:1794	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	12	12	theme	respiratory	1488:1498	arg1	quinone					1500:1506	the major respiratory quinone	1478:1506	the major respiratory quinone in strain C1-1T	1478:1522	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	12	12	theme	respiratory	1488:1498	arg1	MK-9					1456:1459	Menaquinone MK-9	1444:1459	Menaquinone MK-9(H2) (86.4 %)	1444:1472	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	3	13	contain	have	373:376	arg2	apparatus					397:405	a flagellar motile apparatus	378:405	a flagellar motile apparatus	378:405	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	3	13	contain	have	373:376	arg1	Cells					332:336	Cells	332:336	Cells with a size of 0.65-0.9×1.2-1.7 µm	332:371	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	6	14	theme	strain	770:775	arg1	C1-1T					777:781	strain C1-1T	770:781	strain C1-1T	770:781	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	9	15	theme	A.	1165:1166	arg1	113092T					1183:1189	A. glacialis NBRC 113092T	1165:1189	A. glacialis NBRC 113092T	1165:1189	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	2	16	theme	Col	293:295	arg1	Antarctic					321:329	Maritime Antarctic	312:329	Maritime Antarctic	312:329	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	2	16	theme	Col	293:295	arg1	Signy					298:302	Signy	298:302	Signy	298:302	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	2	16	theme	Col	293:295	arg1	Island					304:309	Jane Col, Signy Island	288:309	Island	304:309	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	14	17	theme	novel	1758:1762	arg1	sp					1793:1794	a novel species Arthrobacter polaris sp	1756:1794	a novel species Arthrobacter polaris sp	1756:1794	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	13	18	dep	l-Lys-l-Ala3	1571:1582	arg1	A11.6					1585:1589	A11.6	1585:1589	A11.6	1585:1589	The peptidoglycan type was determined as A3α (l-Lys-l-Ala3; A11.6).
36256564	1	19	theme	fellfield	110:118	arg1	soil					120:123	Antarctic fellfield soil	100:123	Antarctic fellfield soil	100:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	3	20	with	Cells	332:336	arg1	size					345:348	a size	343:348	a size of 0.65-0.9×1.2-1.7 µm	343:371	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	8	21	dep	A.	1035:1036	arg1	glacialis					1038:1046	glacialis	1038:1046	glacialis	1038:1046	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	5	22	theme	glacialis	664:672	arg1	113092T					679:685	Arthrobacter glacialis NBRC 113092T	651:685	Arthrobacter glacialis NBRC 113092T	651:685	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	9	23	theme	NBRC	1178:1181	arg1	113092T					1183:1189	A. glacialis NBRC 113092T	1165:1189	A. glacialis NBRC 113092T	1165:1189	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	13	24	dep	A3α	1566:1568	arg1	l-Lys-l-Ala3					1571:1582	l-Lys-l-Ala3	1571:1582	l-Lys-l-Ala3; A11.6	1571:1589	The peptidoglycan type was determined as A3α (l-Lys-l-Ala3; A11.6).
36256564	6	25	theme	group	896:900	arg1	members					848:854	members	848:854	members of the 'Arthrobacter psychrolactophilus group'	848:901	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	4	26	theme	Optimal	446:452	arg1	conditions					461:470	Optimal growth conditions	446:470	Optimal growth conditions	446:470	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	7	27	theme	58.95 mol	939:947	arg1	%					948:948	58.95 mol%	939:948	58.95 mol%	939:948	The G+C content of genomic DNA was 58.95 mol%.
36256564	7	27	theme	58.95 mol	939:947	arg1	content					912:918	The G+C content	904:918	The G+C content of genomic DNA	904:933	The G+C content of genomic DNA was 58.95 mol%.
36256564	14	28	theme	type	1741:1744	arg1	C1-1T					1703:1707	strain C1-1T	1696:1707	strain C1-1T (=DSM 112353T=CCM 9148T)	1696:1732	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	28	theme	type	1741:1744	arg1	strain					1746:1751	the type strain	1737:1751	the type strain of a novel species Arthrobacter polaris sp	1737:1794	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	6	29	theme	Arthrobacter	864:875	arg1	group					896:900	the 'Arthrobacter psychrolactophilus group'	859:901	the 'Arthrobacter psychrolactophilus group'	859:901	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	7	30	theme	DNA	931:933	arg1	%					948:948	58.95 mol%	939:948	58.95 mol%	939:948	The G+C content of genomic DNA was 58.95 mol%.
36256564	7	30	theme	DNA	931:933	arg1	content					912:918	The G+C content	904:918	The G+C content of genomic DNA	904:933	The G+C content of genomic DNA was 58.95 mol%.
36256564	2	31	theme	soil	236:239	arg1	sample					241:246	a fellfield soil sample	224:246	a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic	224:329	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	4	32	located	observed	477:484	arg1	concentration					515:527	NaCl concentration	510:527	NaCl concentration	510:527	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	4	32	located	observed	477:484	arg2	conditions					461:470	Optimal growth conditions	446:470	Optimal growth conditions	446:470	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	4	32	located	observed	477:484	arg1	pH					499:500	pH 7.0	499:504	pH 7.0	499:504	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	4	32	located	observed	477:484	arg1	medium					554:559	the medium	550:559	the medium	550:559	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	4	32	located	observed	477:484	arg1	15-20 °C					489:496	15-20 °C	489:496	15-20 °C	489:496	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	8	33	theme	original	955:962	arg1	identities					999:1008	The original and orthologous average nucleotide identities	951:1008	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T	951:1059	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	8	33	theme	original	955:962	arg1	%					1072:1072	77.15 %	1066:1072	77.15 %	1066:1072	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	7	34	theme	G+C	908:910	arg1	%					948:948	58.95 mol%	939:948	58.95 mol%	939:948	The G+C content of genomic DNA was 58.95 mol%.
36256564	7	34	theme	G+C	908:910	arg1	content					912:918	The G+C content	904:918	The G+C content of genomic DNA	904:933	The G+C content of genomic DNA was 58.95 mol%.
36256564	14	35	theme	=DSM	1710:1713	arg1	9148T					1727:1731	=DSM 112353T=CCM 9148T	1710:1731	=DSM 112353T=CCM 9148T	1710:1731	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	35	theme	=DSM	1710:1713	arg1	C1-1T					1703:1707	strain C1-1T	1696:1707	strain C1-1T (=DSM 112353T=CCM 9148T)	1696:1732	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	12	36	theme	Menaquinone	1444:1454	arg1	quinone					1500:1506	the major respiratory quinone	1478:1506	the major respiratory quinone in strain C1-1T	1478:1522	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	12	36	theme	Menaquinone	1444:1454	arg1	H2					1461:1462	H2	1461:1462	H2	1461:1462	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	12	36	theme	Menaquinone	1444:1454	arg1	MK-9					1456:1459	Menaquinone MK-9	1444:1459	Menaquinone MK-9(H2) (86.4 %)	1444:1472	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	12	36	theme	Menaquinone	1444:1454	arg1	%					1471:1471	86.4 %	1466:1471	86.4 %	1466:1471	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	8	37	theme	orthologous	968:978	arg1	identities					999:1008	The original and orthologous average nucleotide identities	951:1008	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T	951:1059	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	8	37	theme	orthologous	968:978	arg1	%					1072:1072	77.15 %	1066:1072	77.15 %	1066:1072	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	10	38	theme	lipid	1213:1217	arg1	profile					1219:1225	The polar lipid profile	1203:1225	The polar lipid profile	1203:1225	The polar lipid profile was composed mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid.
36256564	3	39	theme	rod-coccus	421:430	arg1	cycle					439:443	a rod-coccus growth cycle	419:443	a rod-coccus growth cycle	419:443	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	4	40	dep	0.5 	535:538	arg1	to					532:533	to	532:533	to	532:533	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	8	41	theme	nucleotide	988:997	arg1	identities					999:1008	The original and orthologous average nucleotide identities	951:1008	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T	951:1059	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	8	41	theme	nucleotide	988:997	arg1	%					1072:1072	77.15 %	1066:1072	77.15 %	1066:1072	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	0	42	theme	Arthrobacter	0:11	arg1	sp					21:22	Arthrobacter polaris sp	0:22	Arthrobacter polaris sp.	0:23	Arthrobacter polaris sp.
36256564	1	43	theme	Micrococcaceae	71:84	arg1	member					50:55	a new cold-adapted member	31:55	a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil	31:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	1	43	theme	Micrococcaceae	71:84	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	14	44	theme	strain	1696:1701	arg1	9148T					1727:1731	=DSM 112353T=CCM 9148T	1710:1731	=DSM 112353T=CCM 9148T	1710:1731	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	44	theme	strain	1696:1701	arg1	C1-1T					1703:1707	strain C1-1T	1696:1707	strain C1-1T (=DSM 112353T=CCM 9148T)	1696:1732	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	44	theme	strain	1696:1701	arg1	strain					1746:1751	the type strain	1737:1751	the type strain of a novel species Arthrobacter polaris sp	1737:1794	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	8	45	theme	A.	1035:1036	arg1	113092T					1053:1059	A. glacialis NBRC 113092T	1035:1059	A. glacialis NBRC 113092T	1035:1059	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	3	46	theme	flagellar	380:388	arg1	apparatus					397:405	a flagellar motile apparatus	378:405	a flagellar motile apparatus	378:405	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	8	47	theme	NBRC	1048:1051	arg1	113092T					1053:1059	A. glacialis NBRC 113092T	1035:1059	A. glacialis NBRC 113092T	1035:1059	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	2	48	theme	Maritime	312:319	arg1	Antarctic					321:329	Maritime Antarctic	312:329	Maritime Antarctic	312:329	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	2	48	theme	Maritime	312:319	arg1	Island					304:309	Jane Col, Signy Island	288:309	Island	304:309	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	14	49	theme	chemotaxonomic	1648:1661	arg1	characteristics					1663:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	50	theme	sp	1793:1794	arg1	C1-1T					1703:1707	strain C1-1T	1696:1707	strain C1-1T (=DSM 112353T=CCM 9148T)	1696:1732	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	50	theme	sp	1793:1794	arg1	strain					1746:1751	the type strain	1737:1751	the type strain of a novel species Arthrobacter polaris sp	1737:1794	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	51	theme	physiological	1630:1642	arg1	characteristics					1663:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	1	52	theme	new	33:35	arg1	member					50:55	a new cold-adapted member	31:55	a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil	31:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	1	52	theme	new	33:35	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	5	53	theme	C1-1T	592:596	arg1	sequence					580:587	The 16S rRNA gene sequence	562:587	The 16S rRNA gene sequence of C1-1T	562:596	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	14	54	theme	phylogenetic	1616:1627	arg1	characteristics					1663:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	all described phylogenetic, physiological and chemotaxonomic characteristics	1602:1677	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	9	55	theme	DNA-DNA	1113:1119	arg1	values					1133:1138	The digital DNA-DNA relatedness values	1101:1138	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T	1101:1189	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	9	55	theme	DNA-DNA	1113:1119	arg1	%					1200:1200	21.6 %	1195:1200	21.6 %	1195:1200	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	2	56	theme	Jane	288:291	arg1	Antarctic					321:329	Maritime Antarctic	312:329	Maritime Antarctic	312:329	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	2	56	theme	Jane	288:291	arg1	Signy					298:302	Signy	298:302	Signy	298:302	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	2	56	theme	Jane	288:291	arg1	Island					304:309	Jane Col, Signy Island	288:309	Island	304:309	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	11	57	theme	cellular	1365:1372	arg1	acids					1380:1384	The predominant cellular fatty acids	1349:1384	The predominant cellular fatty acids	1349:1384	The predominant cellular fatty acids were anteiso-C15 : 0 (75 %) and anteiso-C17 : 0 (15.2 %).
36256564	5	58	theme	rRNA	570:573	arg1	sequence					580:587	The 16S rRNA gene sequence	562:587	The 16S rRNA gene sequence of C1-1T	562:596	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	9	59	theme	strain	1148:1153	arg1	C1-1T					1155:1159	strain C1-1T	1148:1159	strain C1-1T	1148:1159	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	14	60	theme	polaris	1785:1791	arg1	sp					1793:1794	a novel species Arthrobacter polaris sp	1756:1794	a novel species Arthrobacter polaris sp	1756:1794	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	5	61	theme	%	646:646	arg1	similarity					626:635	the highest pairwise similarity	605:635	the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T	605:685	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	6	62	theme	16S	720:722	arg1	rRNA					724:727	the 16S rRNA	716:727	the 16S rRNA	716:727	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	14	63	theme	species	1764:1770	arg1	sp					1793:1794	a novel species Arthrobacter polaris sp	1756:1794	a novel species Arthrobacter polaris sp	1756:1794	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	12	64	theme	strain	1511:1516	arg1	C1-1T					1518:1522	strain C1-1T	1511:1522	strain C1-1T	1511:1522	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	5	65	theme	Arthrobacter	651:662	arg1	113092T					679:685	Arthrobacter glacialis NBRC 113092T	651:685	Arthrobacter glacialis NBRC 113092T	651:685	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	2	66	theme	fellfield	226:234	arg1	sample					241:246	a fellfield soil sample	224:246	a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic	224:329	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	4	67	theme	growth	454:459	arg1	conditions					461:470	Optimal growth conditions	446:470	Optimal growth conditions	446:470	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	5	68	theme	pairwise	617:624	arg1	similarity					626:635	the highest pairwise similarity	605:635	the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T	605:685	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	1	69	theme	Antarctic	100:108	arg1	soil					120:123	Antarctic fellfield soil	100:123	Antarctic fellfield soil	100:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	12	70	theme	major	1482:1486	arg1	quinone					1500:1506	the major respiratory quinone	1478:1506	the major respiratory quinone in strain C1-1T	1478:1522	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	12	70	theme	major	1482:1486	arg1	MK-9					1456:1459	Menaquinone MK-9	1444:1459	Menaquinone MK-9(H2) (86.4 %)	1444:1472	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	1	71	attach	isolated	86:93	arg1	soil					120:123	Antarctic fellfield soil	100:123	Antarctic fellfield soil	100:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	1	71	attach	isolated	86:93	arg2	Micrococcaceae					71:84	the family Micrococcaceae	60:84	the family Micrococcaceae isolated from Antarctic fellfield soil	60:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	7	72	theme	genomic	923:929	arg1	DNA					931:933	genomic DNA	923:933	genomic DNA	923:933	The G+C content of genomic DNA was 58.95 mol%.
36256564	14	73	theme	112353T=CCM	1715:1725	arg1	9148T					1727:1731	=DSM 112353T=CCM 9148T	1710:1731	=DSM 112353T=CCM 9148T	1710:1731	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	14	73	theme	112353T=CCM	1715:1725	arg1	C1-1T					1703:1707	strain C1-1T	1696:1707	strain C1-1T (=DSM 112353T=CCM 9148T)	1696:1732	Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1T (=DSM 112353T=CCM 9148T) is the type strain of a novel species Arthrobacter polaris sp.
36256564	5	74	theme	NBRC	674:677	arg1	113092T					679:685	Arthrobacter glacialis NBRC 113092T	651:685	Arthrobacter glacialis NBRC 113092T	651:685	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	8	75	theme	average	980:986	arg1	identities					999:1008	The original and orthologous average nucleotide identities	951:1008	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T	951:1059	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	8	75	theme	average	980:986	arg1	%					1072:1072	77.15 %	1066:1072	77.15 %	1066:1072	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	1	76	theme	cold-adapted	37:48	arg1	member					50:55	a new cold-adapted member	31:55	a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil	31:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	1	76	theme	cold-adapted	37:48	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	2	77	attach	isolated	210:217	arg1	sample					241:246	a fellfield soil sample	224:246	a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic	224:329	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	2	77	attach	isolated	210:217	arg2	strain					180:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	6	78	theme	Phylogenetic	688:699	arg1	trees					701:705	Phylogenetic trees	688:705	Phylogenetic trees based on the 16S rRNA and whole-genome sequences	688:754	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	5	79	theme	16S	566:568	arg1	rRNA					570:573	16S rRNA	566:573	The 16S rRNA gene sequence of C1-1T	562:596	The 16S rRNA gene sequence of C1-1T showed the highest pairwise similarity of 98.77 % to Arthrobacter glacialis NBRC 113092T.
36256564	10	80	theme	polar	1207:1211	arg1	profile					1219:1225	The polar lipid profile	1203:1225	The polar lipid profile	1203:1225	The polar lipid profile was composed mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid.
36256564	3	81	theme	growth	432:437	arg1	cycle					439:443	a rod-coccus growth cycle	419:443	a rod-coccus growth cycle	419:443	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	0	82	theme	polaris	13:19	arg1	sp					21:22	Arthrobacter polaris sp	0:22	Arthrobacter polaris sp.	0:23	Arthrobacter polaris sp.
36256564	8	83	theme	strain	1018:1023	arg1	C1-1T					1025:1029	strain C1-1T	1018:1029	strain C1-1T	1018:1029	The original and orthologous average nucleotide identities between strain C1-1T and A. glacialis NBRC 113092T were 77.15 % and 77.38 %, respectively.
36256564	1	84	theme	family	64:69	arg1	Micrococcaceae					71:84	the family Micrococcaceae	60:84	the family Micrococcaceae isolated from Antarctic fellfield soil	60:123	nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil.
36256564	3	85	theme	0.65-0.9×1.2-1.7 µm	353:371	arg1	size					345:348	a size	343:348	a size of 0.65-0.9×1.2-1.7 µm	343:371	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	12	86	from	quinone	1500:1506	arg1	C1-1T					1518:1522	strain C1-1T	1511:1522	strain C1-1T	1511:1522	Menaquinone MK-9(H2) (86.4 %) was the major respiratory quinone in strain C1-1T.
36256564	4	87	theme	NaCl	510:513	arg1	concentration					515:527	NaCl concentration	510:527	NaCl concentration	510:527	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	2	88	theme	non-spore-forming	162:178	arg1	strain					180:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain	126:185	An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1T, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic.
36256564	6	89	theme	genus	798:802	arg1	Arthrobacter					804:815	the genus Arthrobacter	794:815	the genus Arthrobacter	794:815	Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1T belongs to the genus Arthrobacter and is most closely related to members of the 'Arthrobacter psychrolactophilus group'.
36256564	3	90	theme	motile	390:395	arg1	apparatus					397:405	a flagellar motile apparatus	378:405	a flagellar motile apparatus	378:405	Cells with a size of 0.65-0.9×1.2-1.7 µm have a flagellar motile apparatus and exhibit a rod-coccus growth cycle.
36256564	4	91	dep	15-20 °C	489:496	arg1	w/v					542:544	w/v	542:544	w/v	542:544	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	4	91	dep	15-20 °C	489:496	arg1	%					539:539	up to 0.5 %	529:539	up to 0.5 % (w/v)	529:545	Optimal growth conditions were observed at 15-20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium.
36256564	13	92	theme	peptidoglycan	1529:1541	arg1	A3α					1566:1568	A3α	1566:1568	A3α (l-Lys-l-Ala3; A11.6)	1566:1590	The peptidoglycan type was determined as A3α (l-Lys-l-Ala3; A11.6).
36256564	13	92	theme	peptidoglycan	1529:1541	arg1	type					1543:1546	The peptidoglycan type	1525:1546	The peptidoglycan type	1525:1546	The peptidoglycan type was determined as A3α (l-Lys-l-Ala3; A11.6).
36256564	10	93	theme	unidentified	1324:1335	arg1	glycolipid					1337:1346	an unidentified glycolipid	1321:1346	an unidentified glycolipid	1321:1346	The polar lipid profile was composed mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid.
36256564	9	94	dep	A.	1165:1166	arg1	glacialis					1168:1176	glacialis	1168:1176	glacialis	1168:1176	The digital DNA-DNA relatedness values between strain C1-1T and A. glacialis NBRC 113092T was 21.6 %.
36256564	11	95	theme	predominant	1353:1363	arg1	acids					1380:1384	The predominant cellular fatty acids	1349:1384	The predominant cellular fatty acids	1349:1384	The predominant cellular fatty acids were anteiso-C15 : 0 (75 %) and anteiso-C17 : 0 (15.2 %).
37238906	6	0	contain	had	1018:1020	arg1	PSP-Fs					987:992	PSP-Fs	987:992	PSP-Fs (PSPs extracted by FAE)	987:1016	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	6	0	contain	had	1018:1020	arg2	activity					1044:1051	better lipid-lowering activity	1022:1051	better lipid-lowering activity	1022:1051	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	6	0	contain	had	1018:1020	arg1	PSP-Es					952:957	PSP-Es	952:957	PSP-Es (PSPs extracted by EAE)	952:981	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	6	1	theme	better	1022:1027	arg1	activity					1044:1051	better lipid-lowering activity	1022:1051	better lipid-lowering activity	1022:1051	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	11	2	theme	varying	1654:1660	arg1	degrees					1662:1668	varying degrees	1654:1668	varying degrees	1654:1668	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	5	3	theme	rheological	886:896	arg1	properties					898:907	better rheological properties	879:907	better rheological properties	879:907	PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw).
37238906	0	4	from	Properties	53:62	arg1	sibiricum					126:134	Polygonatum sibiricum	114:134	Polygonatum sibiricum	114:134	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	8	5	theme	uronic	1328:1333	arg1	acid					1335:1338	uronic acid	1328:1338	uronic acid	1328:1338	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	7	6	contain	have	1156:1159	arg2	Mw					1172:1173	a moderate Mw	1161:1173	a moderate Mw	1161:1173	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	7	6	contain	have	1156:1159	arg1	PSP-Es					1076:1081	PSP-Es	1076:1081	PSP-Es	1076:1081	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	4	7	theme	glycosidic	808:817	arg1	compositions					824:835	glycosidic bond compositions	808:835	glycosidic bond compositions	808:835	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	3	8	theme	enzyme-assisted	507:521	arg1	EAE					535:537	EAE	535:537	EAE	535:537	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	8	theme	enzyme-assisted	507:521	arg1	extraction					523:532	enzyme-assisted extraction	507:532	enzyme-assisted extraction (EAE)	507:538	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	8	9	theme	acid	1335:1338	arg1	Mw					1322:1323	the Mw	1318:1323	the Mw of uronic acid	1318:1338	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	3	10	theme	extraction	386:395	arg1	extraction					603:612	freeze-thaw-assisted extraction	582:612	freeze-thaw-assisted extraction (FAE)	582:618	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	10	theme	extraction	386:395	arg1	extraction					523:532	enzyme-assisted extraction	507:532	enzyme-assisted extraction (EAE)	507:538	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	10	theme	extraction	386:395	arg1	extraction					560:569	microwave-assisted extraction	541:569	microwave-assisted extraction (MAE)	541:575	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	10	theme	extraction	386:395	arg1	extraction					451:460	alkali extraction	444:460	alkali extraction (AAE)	444:466	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	10	theme	extraction	386:395	arg1	extraction					489:498	ultrasound-assisted extraction	469:498	ultrasound-assisted extraction (UAE)	469:504	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	10	theme	extraction	386:395	arg1	extraction					426:435	hot water extraction	416:435	hot water extraction (HWE)	416:441	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	10	theme	extraction	386:395	arg1	methods					397:403	six extraction methods	382:403	six extraction methods	382:403	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	0	11	from	Effects	0:6	arg1	Activities					79:88	Biological Activities	68:88	Biological Activities	68:88	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	0	11	from	Effects	0:6	arg1	Properties					53:62	Physicochemical Properties	37:62	Physicochemical Properties	37:62	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	4	12	theme	functional	754:763	arg1	compositions					771:782	similar functional group compositions	746:782	similar functional group compositions	746:782	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	0	13	theme	Polysaccharides	93:107	arg1	Activities					79:88	Biological Activities	68:88	Biological Activities	68:88	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	0	13	theme	Polysaccharides	93:107	arg1	Properties					53:62	Physicochemical Properties	37:62	Physicochemical Properties	37:62	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	1	14	contain	have	182:185	arg1	PSPs					176:179	PSPs	176:179	PSPs	176:179	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	1	14	contain	have	182:185	arg1	polysaccharides					159:173	Polygonatum sibiricum polysaccharides	137:173	Polygonatum sibiricum polysaccharides (PSPs)	137:180	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	1	14	contain	have	182:185	arg2	functions					278:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	1	14	contain	have	182:185	arg2	functions					208:216	important biological functions	187:216	important biological functions	187:216	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	4	15	theme	similar	746:752	arg1	compositions					771:782	similar functional group compositions	746:782	similar functional group compositions	746:782	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	11	16	theme	biological	1612:1621	arg1	activity					1623:1630	biological activity	1612:1630	biological activity	1612:1630	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	3	17	theme	microwave-assisted	541:558	arg1	MAE					572:574	MAE	572:574	MAE	572:574	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	17	theme	microwave-assisted	541:558	arg1	extraction					560:569	microwave-assisted extraction	541:569	microwave-assisted extraction (MAE)	541:575	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	5	18	theme	higher	922:927	arg1	Mw					947:948	Mw	947:948	Mw	947:948	PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw).
37238906	5	18	theme	higher	922:927	arg1	weight					939:944	their higher molecular weight	916:944	their higher molecular weight (Mw)	916:949	PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw).
37238906	11	19	theme	different	1558:1566	arg1	methods					1579:1585	different extraction methods	1558:1585	different extraction methods	1558:1585	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	7	20	theme	-radical-scavenging	1223:1241	arg1	activity					1243:1250	1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity	1187:1250	1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity	1187:1250	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	1	21	theme	important	187:195	arg1	functions					208:216	important biological functions	187:216	important biological functions	187:216	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	1	21	theme	important	187:195	arg1	functions					278:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	0	22	theme	Polygonatum	114:124	arg1	sibiricum					126:134	Polygonatum sibiricum	114:134	Polygonatum sibiricum	114:134	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	11	23	theme	polysaccharides	1635:1649	arg1	structure					1598:1606	structure	1598:1606	structure	1598:1606	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	11	23	theme	polysaccharides	1635:1649	arg1	activity					1623:1630	biological activity	1612:1630	biological activity	1612:1630	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	7	24	dep	PSP-Ms	1087:1092	arg1	PSPs					1095:1098	PSPs	1095:1098	PSPs extracted by MAE	1095:1115	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	4	25	theme	group	765:769	arg1	compositions					771:782	similar functional group compositions	746:782	similar functional group compositions	746:782	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	1	26	theme	biological	197:206	arg1	functions					208:216	important biological functions	187:216	important biological functions	187:216	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	1	26	theme	biological	197:206	arg1	functions					278:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	3	27	theme	hot	416:418	arg1	HWE					438:440	HWE	438:440	HWE	438:440	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	27	theme	hot	416:418	arg1	extraction					426:435	hot water extraction	416:435	hot water extraction (HWE)	416:441	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	6	28	dep	PSP-Fs	987:992	arg1	PSPs					995:998	PSPs	995:998	PSPs extracted by FAE	995:1015	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	7	29	theme	uronic	1140:1145	arg1	acid					1147:1150	uronic acid	1140:1150	uronic acid	1140:1150	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	11	30	theme	structure-activity	1723:1740	arg1	relationship					1742:1753	the structure-activity relationship	1719:1753	the structure-activity relationship of PSPs	1719:1761	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	5	31	theme	molecular	929:937	arg1	Mw					947:948	Mw	947:948	Mw	947:948	PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw).
37238906	5	31	theme	molecular	929:937	arg1	weight					939:944	their higher molecular weight	916:944	their higher molecular weight (Mw)	916:949	PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw).
37238906	3	32	theme	water	420:424	arg1	HWE					438:440	HWE	438:440	HWE	438:440	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	32	theme	water	420:424	arg1	extraction					426:435	hot water extraction	416:435	hot water extraction (HWE)	416:441	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	10	33	theme	important	1481:1489	arg1	role					1491:1494	an important role	1478:1494	an important role	1478:1494	In addition, mannose (Man) may play an important role in the immunomodulatory activity.
37238906	8	34	theme	OH-radical-scavenging	1354:1374	arg1	activity					1376:1383	the best OH-radical-scavenging activity	1345:1383	the best OH-radical-scavenging activity	1345:1383	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	8	35	theme	best	1349:1352	arg1	activity					1376:1383	the best OH-radical-scavenging activity	1345:1383	the best OH-radical-scavenging activity	1345:1383	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	3	36	theme	alkali	444:449	arg1	extraction					451:460	alkali extraction	444:460	alkali extraction (AAE)	444:466	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	36	theme	alkali	444:449	arg1	AAE					463:465	AAE	463:465	AAE	463:465	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	0	37	theme	Methods	22:28	arg1	Effects					0:6	Effects	0:6	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.	0:135	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	2	38	contain	have	318:321	arg1	methods					310:316	Different extraction methods	289:316	Different extraction methods	289:316	Different extraction methods have effects on their structures and activities.
37238906	2	38	contain	have	318:321	arg2	effects					323:329	effects	323:329	effects on their structures and activities	323:364	Different extraction methods have effects on their structures and activities.
37238906	11	39	theme	PSPs	1758:1761	arg1	relationship					1742:1753	the structure-activity relationship	1719:1753	the structure-activity relationship of PSPs	1719:1761	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	3	40	theme	freeze-thaw-assisted	582:601	arg1	FAE					615:617	FAE	615:617	FAE	615:617	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	40	theme	freeze-thaw-assisted	582:601	arg1	extraction					603:612	freeze-thaw-assisted extraction	582:612	freeze-thaw-assisted extraction (FAE)	582:618	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	0	41	theme	Extraction	11:20	arg1	Methods					22:28	Extraction Methods	11:28	Extraction Methods	11:28	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	9	42	theme	high-Mw	1390:1396	arg1	PSP-As					1398:1403	The high-Mw PSP-As	1386:1403	The high-Mw PSP-As	1386:1403	The high-Mw PSP-As had the best Fe2+-chelating ability.
37238906	3	43	theme	extract	633:639	arg1	PSPs					641:644	extract PSPs	633:644	extract PSPs	633:644	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	9	44	theme	Fe2+-chelating	1418:1431	arg1	ability					1433:1439	the best Fe2+-chelating ability	1409:1439	the best Fe2+-chelating ability	1409:1439	The high-Mw PSP-As had the best Fe2+-chelating ability.
37238906	3	45	theme	ultrasound-assisted	469:487	arg1	UAE					501:503	UAE	501:503	UAE	501:503	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	45	theme	ultrasound-assisted	469:487	arg1	extraction					489:498	ultrasound-assisted extraction	469:498	ultrasound-assisted extraction (UAE)	469:504	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	0	46	from	Activities	79:88	arg1	sibiricum					126:134	Polygonatum sibiricum	114:134	Polygonatum sibiricum	114:134	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	0	47	dep	Properties	53:62	arg1	the					33:35	the	33:35	the	33:35	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	10	48	theme	immunomodulatory	1503:1518	arg1	activity					1520:1527	the immunomodulatory activity	1499:1527	the immunomodulatory activity	1499:1527	In addition, mannose (Man) may play an important role in the immunomodulatory activity.
37238906	1	49	theme	antioxidation	227:239	arg1	functions					278:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	6	50	theme	lipid-lowering	1029:1042	arg1	activity					1044:1051	better lipid-lowering activity	1022:1051	better lipid-lowering activity	1022:1051	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	8	51	dep	PSP-Hs	1270:1275	arg1	PSPs					1278:1281	PSPs	1278:1281	PSPs extracted by HWE	1278:1298	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	8	52	with	PSP-Hs	1270:1275	arg1	Mw					1322:1323	the Mw	1318:1323	the Mw of uronic acid	1318:1338	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	11	53	theme	extraction	1568:1577	arg1	methods					1579:1585	different extraction methods	1558:1585	different extraction methods	1558:1585	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	11	54	dep	structure	1598:1606	arg1	the					1594:1596	the	1594:1596	the	1594:1596	These results indicate that different extraction methods affect the structure and biological activity of polysaccharides to varying degrees, and these results are helpful for understanding the structure-activity relationship of PSPs.
37238906	9	55	theme	best	1413:1416	arg1	ability					1433:1439	the best Fe2+-chelating ability	1409:1439	the best Fe2+-chelating ability	1409:1439	The high-Mw PSP-As had the best Fe2+-chelating ability.
37238906	3	56	used	used	625:628	arg2	extraction					489:498	ultrasound-assisted extraction	469:498	ultrasound-assisted extraction (UAE)	469:504	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	56	used	used	625:628	arg2	extraction					523:532	enzyme-assisted extraction	507:532	enzyme-assisted extraction (EAE)	507:538	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	56	used	used	625:628	arg2	extraction					603:612	freeze-thaw-assisted extraction	582:612	freeze-thaw-assisted extraction (FAE)	582:618	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	56	used	used	625:628	arg2	extraction					560:569	microwave-assisted extraction	541:569	microwave-assisted extraction (MAE)	541:575	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	56	used	used	625:628	arg2	methods					397:403	six extraction methods	382:403	six extraction methods	382:403	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	56	used	used	625:628	arg2	extraction					426:435	hot water extraction	416:435	hot water extraction (HWE)	416:441	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	3	56	used	used	625:628	arg2	extraction					451:460	alkali extraction	444:460	alkali extraction (AAE)	444:466	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	8	57	with	PSP-Fs	1305:1310	arg1	Mw					1322:1323	the Mw	1318:1323	the Mw of uronic acid	1318:1338	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	0	58	theme	Physicochemical	37:51	arg1	Properties					53:62	Physicochemical Properties	37:62	Physicochemical Properties	37:62	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	0	59	from	sibiricum	126:134	arg1	Activities					79:88	Biological Activities	68:88	Biological Activities	68:88	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	0	59	from	sibiricum	126:134	arg1	Polysaccharides					93:107	Polysaccharides	93:107	Polysaccharides from Polygonatum sibiricum	93:134	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	0	59	from	sibiricum	126:134	arg1	Properties					53:62	Physicochemical Properties	37:62	Physicochemical Properties	37:62	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	4	60	theme	bond	819:822	arg1	compositions					824:835	glycosidic bond compositions	808:835	glycosidic bond compositions	808:835	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	7	61	dep	activity	1243:1250	arg1	had					1176:1178	had	1176:1178	had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity	1176:1250	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	1	62	theme	Polygonatum	137:147	arg1	PSPs					176:179	PSPs	176:179	PSPs	176:179	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	1	62	theme	Polygonatum	137:147	arg1	polysaccharides					159:173	Polygonatum sibiricum polysaccharides	137:173	Polygonatum sibiricum polysaccharides (PSPs)	137:180	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	8	63	contain	had	1341:1343	arg1	PSP-Hs					1270:1275	PSP-Hs	1270:1275	PSP-Hs (PSPs extracted by HWE)	1270:1299	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	8	63	contain	had	1341:1343	arg2	activity					1376:1383	the best OH-radical-scavenging activity	1345:1383	the best OH-radical-scavenging activity	1345:1383	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	8	63	contain	had	1341:1343	arg1	PSP-Fs					1305:1310	PSP-Fs	1305:1310	PSP-Fs	1305:1310	On the contrary, PSP-Hs (PSPs extracted by HWE) and PSP-Fs, with the Mw of uronic acid, had the best OH-radical-scavenging activity.
37238906	3	64	theme	structure-activity	657:674	arg1	relationships					676:688	their structure-activity relationships	651:688	their structure-activity relationships	651:688	In this study, six extraction methods, including hot water extraction (HWE), alkali extraction (AAE), ultrasound-assisted extraction (UAE), enzyme-assisted extraction (EAE), microwave-assisted extraction (MAE), and freeze-thaw-assisted extraction (FAE) were used to extract PSPs, and their structure-activity relationships were analyzed.
37238906	2	65	theme	extraction	299:308	arg1	methods					310:316	Different extraction methods	289:316	Different extraction methods	289:316	Different extraction methods have effects on their structures and activities.
37238906	1	66	theme	sibiricum	149:157	arg1	PSPs					176:179	PSPs	176:179	PSPs	176:179	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	1	66	theme	sibiricum	149:157	arg1	polysaccharides					159:173	Polygonatum sibiricum polysaccharides	137:173	Polygonatum sibiricum polysaccharides (PSPs)	137:180	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	6	67	theme	lower	1066:1070	arg1	Mw					1072:1073	their lower Mw	1060:1073	their lower Mw	1060:1073	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	1	68	theme	immunomodulatory	242:257	arg1	functions					278:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
37238906	9	69	contain	had	1405:1407	arg2	ability					1433:1439	the best Fe2+-chelating ability	1409:1439	the best Fe2+-chelating ability	1409:1439	The high-Mw PSP-As had the best Fe2+-chelating ability.
37238906	9	69	contain	had	1405:1407	arg1	PSP-As					1398:1403	The high-Mw PSP-As	1386:1403	The high-Mw PSP-As	1386:1403	The high-Mw PSP-As had the best Fe2+-chelating ability.
37238906	2	70	theme	Different	289:297	arg1	methods					310:316	Different extraction methods	289:316	Different extraction methods	289:316	Different extraction methods have effects on their structures and activities.
37238906	5	71	theme	better	879:884	arg1	properties					898:907	better rheological properties	879:907	better rheological properties	879:907	PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw).
37238906	4	72	theme	thermal	785:791	arg1	stability					793:801	thermal stability	785:801	thermal stability	785:801	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	0	73	theme	Biological	68:77	arg1	Activities					79:88	Biological Activities	68:88	Biological Activities	68:88	Effects of Extraction Methods on the Physicochemical Properties and Biological Activities of Polysaccharides from Polygonatum sibiricum.
37238906	6	74	dep	PSP-Es	952:957	arg1	PSPs					960:963	PSPs	960:963	PSPs extracted by EAE	960:980	PSP-Es (PSPs extracted by EAE) and PSP-Fs (PSPs extracted by FAE) had better lipid-lowering activity due to their lower Mw.
37238906	7	75	contain	contain	1132:1138	arg2	acid					1147:1150	uronic acid	1140:1150	uronic acid	1140:1150	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	7	75	contain	contain	1132:1138	arg1	PSP-Ms					1087:1092	PSP-Ms	1087:1092	PSP-Ms (PSPs extracted by MAE)	1087:1116	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	7	75	contain	contain	1132:1138	arg1	PSP-Es					1076:1081	PSP-Es	1076:1081	PSP-Es	1076:1081	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	7	76	theme	moderate	1163:1170	arg1	Mw					1172:1173	a moderate Mw	1161:1173	a moderate Mw	1161:1173	PSP-Es and PSP-Ms (PSPs extracted by MAE), which do not contain uronic acid and have a moderate Mw, had better 1,1-diphenyl-2-picrylhydrazyl (DPPH)-radical-scavenging activity.
37238906	2	77	from	effects	323:329	arg1	structures					340:349	structures	340:349	structures	340:349	Different extraction methods have effects on their structures and activities.
37238906	2	77	from	effects	323:329	arg1	activities					355:364	activities	355:364	activities	355:364	Different extraction methods have effects on their structures and activities.
37238906	5	78	dep	PSP-As	838:843	arg1	PSPs					846:849	PSPs	846:849	PSPs extracted by AAE	846:866	PSP-As (PSPs extracted by AAE) exhibited better rheological properties due to their higher molecular weight (Mw).
37238906	4	79	contain	had	742:744	arg1	PSPs					737:740	all six PSPs	729:740	all six PSPs	729:740	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	4	79	contain	had	742:744	arg2	compositions					824:835	glycosidic bond compositions	808:835	glycosidic bond compositions	808:835	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	4	79	contain	had	742:744	arg2	stability					793:801	thermal stability	785:801	thermal stability	785:801	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	4	79	contain	had	742:744	arg2	compositions					771:782	similar functional group compositions	746:782	similar functional group compositions	746:782	The results showed that all six PSPs had similar functional group compositions, thermal stability, and glycosidic bond compositions.
37238906	1	80	theme	hypolipidemic	264:276	arg1	functions					278:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	antioxidation, immunomodulatory, and hypolipidemic functions	227:286	Polygonatum sibiricum polysaccharides (PSPs) have important biological functions, such as antioxidation, immunomodulatory, and hypolipidemic functions.
36192988	0	0	theme	microbiota	81:90	arg1	structure					102:110	the gut microbiota community structure	73:110	the gut microbiota community structure in mice	73:118	Litchi thaumatin-like protein induced the liver inflammation and altered the gut microbiota community structure in mice.
36192988	5	1	from	%	842:842	arg1	group					886:890	high-dose group	876:890	high-dose group	876:890	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	3	2	from	activity	443:450	arg1	cells					464:468	RAW264.7 cells	455:468	RAW264.7 cells	455:468	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	1	3	theme	thaumatin-like	161:174	arg1	LcTLP					185:189	LcTLP	185:189	LcTLP	185:189	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	1	3	theme	thaumatin-like	161:174	arg1	protein					176:182	litchi thaumatin-like protein	154:182	litchi thaumatin-like protein (LcTLP)	154:190	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	1	4	theme	reaction	238:245	arg1	cause					217:221	the cause	213:221	the cause of the adverse reaction after excessive consumption of litchi	213:283	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	1	4	theme	reaction	238:245	arg1	activity					142:149	The pro-inflammation activity	121:149	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP)	121:190	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	0	5	theme	gut	77:79	arg1	structure					102:110	the gut microbiota community structure	73:110	the gut microbiota community structure in mice	73:118	Litchi thaumatin-like protein induced the liver inflammation and altered the gut microbiota community structure in mice.
36192988	5	6	from	TNF-α	772:776	arg1	liver					813:817	liver	813:817	liver	813:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	7	theme	NF-κB	983:987	arg1	upregulation					918:929	the upregulation	914:929	the upregulation of p-p65 and p-p38 levels	914:955	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	7	theme	NF-κB	983:987	arg1	activation					969:978	further activation	961:978	further activation of NF-κB and MAPK signaling pathways	961:1015	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	3	8	from	increase	490:497	arg1	factor-α					544:551	tumor necrosis factor-α	529:551	tumor necrosis factor-α (TNF-α)	529:559	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	8	from	increase	490:497	arg1	interleukin-1β					562:575	interleukin-1β	562:575	interleukin-1β (IL-1β)	562:583	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	8	from	increase	490:497	arg1	interleukin-6					589:601	interleukin-6	589:601	interleukin-6 (IL-6)	589:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	8	from	increase	490:497	arg1	cytokines					519:527	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	1	9	theme	protein	176:182	arg1	cause					217:221	the cause	213:221	the cause of the adverse reaction after excessive consumption of litchi	213:283	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	1	9	theme	protein	176:182	arg1	activity					142:149	The pro-inflammation activity	121:149	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP)	121:190	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	5	10	theme	high-dose	876:884	arg1	group					886:890	high-dose group	876:890	high-dose group	876:890	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	4	11	dep	cytoplasm	696:704	arg1	the					682:684	the	682:684	the	682:684	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	5	12	from	%	859:859	arg1	group					886:890	high-dose group	876:890	high-dose group	876:890	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	6	13	theme	Bacteroidota	1106:1117	arg1	reduction					1093:1101	the reduction	1089:1101	the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels	1089:1252	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	4	14	theme	liver	744:748	arg1	tissue					750:755	liver tissue	744:755	liver tissue	744:755	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	1	15	theme	excessive	253:261	arg1	consumption					263:273	excessive consumption	253:273	excessive consumption of litchi	253:283	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	0	16	theme	community	92:100	arg1	structure					102:110	the gut microbiota community structure	73:110	the gut microbiota community structure in mice	73:118	Litchi thaumatin-like protein induced the liver inflammation and altered the gut microbiota community structure in mice.
36192988	5	17	from	interleukin-10	795:808	arg1	liver					813:817	liver	813:817	liver	813:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	18	theme	due	907:909	arg1	%					842:842	60.43%	837:842	60.43%	837:842	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	19	from	%	850:850	arg1	group					886:890	high-dose group	876:890	high-dose group	876:890	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	4	20	theme	edematous	686:694	arg1	cytoplasm					696:704	edematous cytoplasm	686:704	edematous cytoplasm	686:704	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	6	21	theme	cytokines	1237:1245	arg1	levels					1247:1252	inflammation cytokines levels	1224:1252	inflammation cytokines levels	1224:1252	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	5	22	theme	IL-6	786:789	arg1	levels					762:767	The levels	758:767	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver	758:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	3	23	theme	tumor	529:533	arg1	factor-α					544:551	tumor necrosis factor-α	529:551	tumor necrosis factor-α (TNF-α)	529:559	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	23	theme	tumor	529:533	arg1	TNF-α					554:558	TNF-α	554:558	TNF-α	554:558	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	23	theme	tumor	529:533	arg1	cytokines					519:527	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	24	theme	pro-inflammation	426:441	arg1	activity					443:450	the pro-inflammation activity	422:450	the pro-inflammation activity in RAW264.7 cells	422:468	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	5	25	from	IL-6	786:789	arg1	liver					813:817	liver	813:817	liver	813:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	26	theme	TNF-α	772:776	arg1	levels					762:767	The levels	758:767	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver	758:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	27	from	%	871:871	arg1	group					886:890	high-dose group	876:890	high-dose group	876:890	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	3	28	theme	necrosis	535:542	arg1	factor-α					544:551	tumor necrosis factor-α	529:551	tumor necrosis factor-α (TNF-α)	529:559	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	28	theme	necrosis	535:542	arg1	TNF-α					554:558	TNF-α	554:558	TNF-α	554:558	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	28	theme	necrosis	535:542	arg1	cytokines					519:527	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	0	29	theme	thaumatin-like	7:20	arg1	protein					22:28	Litchi thaumatin-like protein	0:28	Litchi thaumatin-like protein	0:28	Litchi thaumatin-like protein induced the liver inflammation and altered the gut microbiota community structure in mice.
36192988	2	30	theme	pro-inflammation	311:326	arg1	activity					328:335	the pro-inflammation activity	307:335	the pro-inflammation activity of LcTLP	307:344	This study evaluated the pro-inflammation activity of LcTLP in vitro and in vivo.
36192988	4	31	theme	pathological	648:659	arg1	examination					661:671	pathological examination	648:671	pathological examination	648:671	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	0	32	theme	Litchi	0:5	arg1	protein					22:28	Litchi thaumatin-like protein	0:28	Litchi thaumatin-like protein	0:28	Litchi thaumatin-like protein induced the liver inflammation and altered the gut microbiota community structure in mice.
36192988	4	33	from	supplementation	623:637	arg1	mice					642:645	mice	642:645	mice	642:645	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	5	34	theme	interleukin-10	795:808	arg1	levels					762:767	The levels	758:767	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver	758:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	1	35	theme	litchi	278:283	arg1	consumption					263:273	excessive consumption	253:273	excessive consumption of litchi	253:283	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	4	36	from	accumulation	728:739	arg1	tissue					750:755	liver tissue	744:755	liver tissue	744:755	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	5	37	theme	MAPK	993:996	arg1	pathways					1008:1015	MAPK signaling pathways	993:1015	MAPK signaling pathways	993:1015	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	3	38	dep	cytokines	519:527	arg1	TNF-α					554:558	TNF-α	554:558	TNF-α	554:558	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	38	dep	cytokines	519:527	arg1	cytokines					519:527	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	38	dep	cytokines	519:527	arg1	interleukin-6					589:601	interleukin-6	589:601	interleukin-6 (IL-6)	589:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	38	dep	cytokines	519:527	arg1	interleukin-1β					562:575	interleukin-1β	562:575	interleukin-1β (IL-1β)	562:583	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	38	dep	cytokines	519:527	arg1	IL-6					604:607	IL-6	604:607	IL-6	604:607	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	38	dep	cytokines	519:527	arg1	factor-α					544:551	tumor necrosis factor-α	529:551	tumor necrosis factor-α (TNF-α)	529:559	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	38	dep	cytokines	519:527	arg1	IL-1β					578:582	IL-1β	578:582	IL-1β	578:582	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	0	39	theme	liver	42:46	arg1	inflammation					48:59	the liver inflammation	38:59	the liver inflammation	38:59	Litchi thaumatin-like protein induced the liver inflammation and altered the gut microbiota community structure in mice.
36192988	5	40	theme	signaling	998:1006	arg1	pathways					1008:1015	MAPK signaling pathways	993:1015	MAPK signaling pathways	993:1015	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	6	41	theme	inflammation	1224:1235	arg1	levels					1247:1252	inflammation cytokines levels	1224:1252	inflammation cytokines levels	1224:1252	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	5	42	theme	pathways	1008:1015	arg1	upregulation					918:929	the upregulation	914:929	the upregulation of p-p65 and p-p38 levels	914:955	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	42	theme	pathways	1008:1015	arg1	activation					969:978	further activation	961:978	further activation of NF-κB and MAPK signaling pathways	961:1015	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	43	theme	levels	950:955	arg1	upregulation					918:929	the upregulation	914:929	the upregulation of p-p65 and p-p38 levels	914:955	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	43	theme	levels	950:955	arg1	activation					969:978	further activation	961:978	further activation of NF-κB and MAPK signaling pathways	961:1015	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	3	44	theme	RAW264.7	455:462	arg1	cells					464:468	RAW264.7 cells	455:468	RAW264.7 cells	455:468	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	5	45	theme	IL-1β	779:783	arg1	levels					762:767	The levels	758:767	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver	758:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	6	46	theme	gut	1065:1067	arg1	microbiota					1069:1078	gut microbiota	1065:1078	gut microbiota	1065:1078	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	5	47	from	levels	762:767	arg1	liver					813:817	liver	813:817	liver	813:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	48	theme	further	961:967	arg1	activation					969:978	further activation	961:978	further activation of NF-κB and MAPK signaling pathways	961:1015	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	5	49	from	liver	813:817	arg1	levels					762:767	The levels	758:767	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver	758:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	2	50	theme	LcTLP	340:344	arg1	activity					328:335	the pro-inflammation activity	307:335	the pro-inflammation activity of LcTLP	307:344	This study evaluated the pro-inflammation activity of LcTLP in vitro and in vivo.
36192988	6	51	theme	abundances	1175:1184	arg1	reduction					1093:1101	the reduction	1089:1101	the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels	1089:1252	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	6	52	theme	norank_f__Lachnospiraceae	1149:1173	arg1	abundances					1175:1184	norank_f__Lachnospiraceae abundances	1149:1184	norank_f__Lachnospiraceae abundances	1149:1184	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	1	53	theme	pro-inflammation	125:140	arg1	cause					217:221	the cause	213:221	the cause of the adverse reaction after excessive consumption of litchi	213:283	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	1	53	theme	pro-inflammation	125:140	arg1	activity					142:149	The pro-inflammation activity	121:149	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP)	121:190	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	6	54	theme	microbiota	1069:1078	arg1	composition					1050:1060	the composition	1046:1060	the composition of gut microbiota	1046:1078	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	0	55	from	structure	102:110	arg1	mice					115:118	mice	115:118	mice	115:118	Litchi thaumatin-like protein induced the liver inflammation and altered the gut microbiota community structure in mice.
36192988	4	56	theme	inflammatory	710:721	arg1	accumulation					728:739	inflammatory cell accumulation	710:739	inflammatory cell accumulation	710:739	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	4	57	theme	cell	723:726	arg1	accumulation					728:739	inflammatory cell accumulation	710:739	inflammatory cell accumulation	710:739	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	4	58	theme	LcTLP	617:621	arg1	supplementation					623:637	LcTLP supplementation	617:637	LcTLP supplementation in mice	617:645	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	6	59	theme	norank_f__Muribaculaceae	1120:1143	arg1	reduction					1093:1101	the reduction	1089:1101	the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels	1089:1252	Additionally, LcTLP altered the composition of gut microbiota, such as the reduction of Bacteroidota, norank_f__Muribaculaceae and norank_f__Lachnospiraceae abundances which were negatively correlated with inflammation cytokines levels.
36192988	3	60	theme	pro-inflammation	502:517	arg1	factor-α					544:551	tumor necrosis factor-α	529:551	tumor necrosis factor-α (TNF-α)	529:559	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	60	theme	pro-inflammation	502:517	arg1	interleukin-1β					562:575	interleukin-1β	562:575	interleukin-1β (IL-1β)	562:583	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	60	theme	pro-inflammation	502:517	arg1	interleukin-6					589:601	interleukin-6	589:601	interleukin-6 (IL-6)	589:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	3	60	theme	pro-inflammation	502:517	arg1	cytokines					519:527	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6)	502:608	Results showed that LcTLP digested in vitro exhibited the pro-inflammation activity in RAW264.7 cells, as evidenced by an increase in pro-inflammation cytokines tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6).
36192988	4	61	from	cytoplasm	696:704	arg1	tissue					750:755	liver tissue	744:755	liver tissue	744:755	After LcTLP supplementation in mice, pathological examination revealed the edematous cytoplasm and inflammatory cell accumulation in liver tissue.
36192988	5	62	from	IL-1β	779:783	arg1	liver					813:817	liver	813:817	liver	813:817	The levels of TNF-α, IL-1β, IL-6 and interleukin-10 in liver also increased by 60.43%, 74.86%, 120.18% and 129.91% in high-dose group, respectively, due to the upregulation of p-p65 and p-p38 levels and further activation of NF-κB and MAPK signaling pathways.
36192988	1	63	theme	litchi	154:159	arg1	LcTLP					185:189	LcTLP	185:189	LcTLP	185:189	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	1	63	theme	litchi	154:159	arg1	protein					176:182	litchi thaumatin-like protein	154:182	litchi thaumatin-like protein (LcTLP)	154:190	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36192988	1	64	theme	adverse	230:236	arg1	reaction					238:245	the adverse reaction	226:245	the adverse reaction	226:245	The pro-inflammation activity of litchi thaumatin-like protein (LcTLP) was speculated to be the cause of the adverse reaction after excessive consumption of litchi.
36008675	4	0	theme	molecular	666:674	arg1	all					607:609	all	607:609	all	607:609	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	0	theme	molecular	666:674	arg1	PAMPs					686:690	PAMPs	686:690	PAMPs	686:690	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	0	theme	molecular	666:674	arg1	patterns					676:683	pathogen-associated molecular patterns	646:683	pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells	646:775	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	0	theme	molecular	666:674	arg1	components					628:637	the cell wall components	614:637	the cell wall components	614:637	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	5	1	theme	strong	1165:1170	arg1	response					1203:1210	a strong pro-inflammatory innate immune response	1163:1210	a strong pro-inflammatory innate immune response	1163:1210	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	0	2	theme	PAMP	76:79	arg1	Exposure					81:88	Cell Wall PAMP Exposure	66:88	Cell Wall PAMP Exposure	66:88	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	4	3	theme	wall	801:804	arg1	remodeling					778:787	remodeling	778:787	remodeling of the cell wall	778:804	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	2	4	theme	cell	330:333	arg1	structure					313:321	structure	313:321	structure	313:321	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	2	4	theme	cell	330:333	arg1	shape					303:307	overall shape	295:307	overall shape	295:307	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	5	5	theme	pro-inflammatory	1172:1187	arg1	response					1203:1210	a strong pro-inflammatory innate immune response	1163:1210	a strong pro-inflammatory innate immune response	1163:1210	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	7	6	theme	resting	1470:1476	arg1	cells					1518:1522	both resting and environmentally adapted C. albicans cells	1465:1522	both resting and environmentally adapted C. albicans cells	1465:1522	In this chapter, we outline techniques to assess cell wall components in both resting and environmentally adapted C. albicans cells.
36008675	2	7	gly	glycosylated	368:379	arg1	mannoproteins					381:393	highly glycosylated mannoproteins	361:393	highly glycosylated mannoproteins	361:393	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	3	8	theme	static	504:509	arg1	structure					511:519	a static structure	502:519	a static structure	502:519	However, the cell wall is not a static structure but is constantly being remodeled in response to the external environment.
36008675	3	8	theme	static	504:509	arg1	wall					490:493	the cell wall	481:493	the cell wall	481:493	However, the cell wall is not a static structure but is constantly being remodeled in response to the external environment.
36008675	5	9	theme	innate	1189:1194	arg1	response					1203:1210	a strong pro-inflammatory innate immune response	1163:1210	a strong pro-inflammatory innate immune response	1163:1210	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	6	10	dep	impact	1303:1308	arg1	has					1315:1317	has	1315:1317	has on the host-pathogen interaction	1315:1350	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	5	11	theme	standard	897:904	arg1	media					906:910	standard media	897:910	standard media	897:910	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	7	12	dep	C.	1506:1507	arg1	albicans					1509:1516	albicans	1509:1516	albicans	1509:1516	In this chapter, we outline techniques to assess cell wall components in both resting and environmentally adapted C. albicans cells.
36008675	4	13	theme	pathogen-associated	646:664	arg1	all					607:609	all	607:609	all	607:609	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	13	theme	pathogen-associated	646:664	arg1	PAMPs					686:690	PAMPs	686:690	PAMPs	686:690	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	13	theme	pathogen-associated	646:664	arg1	patterns					676:683	pathogen-associated molecular patterns	646:683	pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells	646:775	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	13	theme	pathogen-associated	646:664	arg1	components					628:637	the cell wall components	614:637	the cell wall components	614:637	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	7	14	from	components	1451:1460	arg1	cells					1518:1522	both resting and environmentally adapted C. albicans cells	1465:1522	both resting and environmentally adapted C. albicans cells	1465:1522	In this chapter, we outline techniques to assess cell wall components in both resting and environmentally adapted C. albicans cells.
36008675	6	15	theme	environmental	1227:1239	arg1	adaptation					1241:1250	environmental adaptation	1227:1250	environmental adaptation	1227:1250	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	16	theme	much	1377:1380	arg1	research					1382:1389	much research	1377:1389	much research	1377:1389	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	4	17	theme	receptors	729:737	arg1	variety					718:724	a variety	716:724	a variety of receptors	716:737	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	17	theme	receptors	729:737	arg1	receptors					729:737	receptors	729:737	receptors	729:737	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	18	theme	cell	618:621	arg1	components					628:637	the cell wall components	614:637	the cell wall components	614:637	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	0	19	theme	Candida	49:55	arg1	albicans					57:64	Quantifying Candida albicans	37:64	Quantifying Candida albicans	37:64	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	5	20	theme	acidic	1011:1016	arg1	environments					1018:1029	acidic environments	1011:1029	acidic environments as encountered during colonization of the female reproductive tract	1011:1097	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	5	21	theme	cell	1139:1142	arg1	surface					1144:1150	the fungal cell surface	1128:1150	the fungal cell surface	1128:1150	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	5	22	from	system	982:987	arg1	PAMPs					953:957	its major cell wall PAMPs	933:957	its major cell wall PAMPs from the innate immune system	933:987	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	6	23	theme	subsequent	1292:1301	arg1	impact					1303:1308	the subsequent impact	1288:1308	the subsequent impact this has on the host-pathogen interaction	1288:1350	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	23	theme	subsequent	1292:1301	arg1	subject					1366:1372	the subject	1362:1372	the subject of much research	1362:1389	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	23	theme	subsequent	1292:1301	arg1	impact					1217:1222	The impact	1213:1222	The impact of environmental adaptation on fungal cell wall remodeling	1213:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	4	24	theme	cell	796:799	arg1	wall					801:804	the cell wall	792:804	the cell wall	792:804	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	0	25	dep	Exposure	81:88	arg1	Methods					26:32	Molecular and Microscopic Methods	0:32	Molecular and Microscopic Methods of Quantifying Candida albicans	0:64	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	5	26	theme	reproductive	1080:1091	arg1	tract					1093:1097	the female reproductive tract	1069:1097	the female reproductive tract	1069:1097	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	0	27	theme	Molecular	0:8	arg1	Methods					26:32	Molecular and Microscopic Methods	0:32	Molecular and Microscopic Methods of Quantifying Candida albicans	0:64	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	5	28	theme	tract	1093:1097	arg1	colonization					1053:1064	colonization	1053:1064	colonization of the female reproductive tract	1053:1097	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	2	29	theme	overall	295:301	arg1	shape					303:307	overall shape	295:307	overall shape	295:307	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	6	30	theme	cell	1262:1265	arg1	remodeling					1272:1281	fungal cell wall remodeling	1255:1281	fungal cell wall remodeling	1255:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	2	31	theme	outer	346:350	arg1	layer					352:356	the outer layer	342:356	the outer layer of highly glycosylated mannoproteins	342:393	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	5	32	from	growth	1001:1006	arg1	environments					1018:1029	acidic environments	1011:1029	acidic environments as encountered during colonization of the female reproductive tract	1011:1097	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	6	33	theme	wall	1267:1270	arg1	remodeling					1272:1281	fungal cell wall remodeling	1255:1281	fungal cell wall remodeling	1255:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	4	34	theme	cells	771:775	arg1	surface					746:752	the surface	742:752	the surface of innate immune cells	742:775	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	1	35	theme	cell	95:98	arg1	structure					143:151	a multilayered structure	128:151	a multilayered structure consisting of polysaccharides and proteins	128:194	The cell wall of Candida albicans is a multilayered structure consisting of polysaccharides and proteins.
36008675	1	35	theme	cell	95:98	arg1	wall					100:103	The cell wall	91:103	The cell wall of Candida albicans	91:123	The cell wall of Candida albicans is a multilayered structure consisting of polysaccharides and proteins.
36008675	5	36	theme	major	937:941	arg1	PAMPs					953:957	its major cell wall PAMPs	933:957	its major cell wall PAMPs from the innate immune system	933:987	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	2	37	theme	key	404:406	arg1	adhesion					439:446	cell adhesion	434:446	cell adhesion required for virulence	434:469	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	2	37	theme	key	404:406	arg1	traits					419:424	key functional traits	404:424	key functional traits such as cell adhesion required for virulence	404:469	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	0	38	theme	Microscopic	14:24	arg1	Methods					26:32	Molecular and Microscopic Methods	0:32	Molecular and Microscopic Methods of Quantifying Candida albicans	0:64	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	5	39	theme	key	1100:1102	arg1	PAMPs					1104:1108	key PAMPs	1100:1108	key PAMPs	1100:1108	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	4	40	theme	host-pathogen	840:852	arg1	interaction					854:864	the host-pathogen interaction	836:864	the host-pathogen interaction	836:864	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	7	41	theme	cell	1441:1444	arg1	components					1451:1460	cell wall components	1441:1460	cell wall components in both resting and environmentally adapted C. albicans cells	1441:1522	In this chapter, we outline techniques to assess cell wall components in both resting and environmentally adapted C. albicans cells.
36008675	3	42	theme	cell	485:488	arg1	structure					511:519	a static structure	502:519	a static structure	502:519	However, the cell wall is not a static structure but is constantly being remodeled in response to the external environment.
36008675	3	42	theme	cell	485:488	arg1	wall					490:493	the cell wall	481:493	the cell wall	481:493	However, the cell wall is not a static structure but is constantly being remodeled in response to the external environment.
36008675	7	43	theme	adapted	1498:1504	arg1	cells					1518:1522	both resting and environmentally adapted C. albicans cells	1465:1522	both resting and environmentally adapted C. albicans cells	1465:1522	In this chapter, we outline techniques to assess cell wall components in both resting and environmentally adapted C. albicans cells.
36008675	0	44	theme	Quantifying	37:47	arg1	albicans					57:64	Quantifying Candida albicans	37:64	Quantifying Candida albicans	37:64	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	6	45	theme	fungal	1255:1260	arg1	remodeling					1272:1281	fungal cell wall remodeling	1255:1281	fungal cell wall remodeling	1255:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	7	46	theme	wall	1446:1449	arg1	components					1451:1460	cell wall components	1441:1460	cell wall components in both resting and environmentally adapted C. albicans cells	1441:1522	In this chapter, we outline techniques to assess cell wall components in both resting and environmentally adapted C. albicans cells.
36008675	4	47	theme	immune	764:769	arg1	cells					771:775	innate immune cells	757:775	innate immune cells	757:775	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	48	theme	wall	623:626	arg1	components					628:637	the cell wall components	614:637	the cell wall components	614:637	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	2	49	theme	mannoproteins	381:393	arg1	layer					352:356	the outer layer	342:356	the outer layer of highly glycosylated mannoproteins	342:393	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	6	50	theme	host-pathogen	1326:1338	arg1	interaction					1340:1350	the host-pathogen interaction	1322:1350	the host-pathogen interaction	1322:1350	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	4	51	theme	innate	757:762	arg1	cells					771:775	innate immune cells	757:775	innate immune cells	757:775	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	2	52	theme	glycosylated	368:379	arg1	mannoproteins					381:393	highly glycosylated mannoproteins	361:393	highly glycosylated mannoproteins	361:393	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	0	53	theme	albicans	57:64	arg1	Methods					26:32	Molecular and Microscopic Methods	0:32	Molecular and Microscopic Methods of Quantifying Candida albicans	0:64	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	6	54	from	impact	1217:1222	arg1	remodeling					1272:1281	fungal cell wall remodeling	1255:1281	fungal cell wall remodeling	1255:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	55	theme	adaptation	1241:1250	arg1	impact					1303:1308	the subsequent impact	1288:1308	the subsequent impact this has on the host-pathogen interaction	1288:1350	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	55	theme	adaptation	1241:1250	arg1	impact					1217:1222	The impact	1213:1222	The impact of environmental adaptation on fungal cell wall remodeling	1213:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	55	theme	adaptation	1241:1250	arg1	subject					1366:1372	the subject	1362:1372	the subject of much research	1362:1389	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	7	56	theme	C.	1506:1507	arg1	cells					1518:1522	both resting and environmentally adapted C. albicans cells	1465:1522	both resting and environmentally adapted C. albicans cells	1465:1522	In this chapter, we outline techniques to assess cell wall components in both resting and environmentally adapted C. albicans cells.
36008675	5	57	theme	wall	948:951	arg1	PAMPs					953:957	its major cell wall PAMPs	933:957	its major cell wall PAMPs from the innate immune system	933:987	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	2	58	theme	cell	434:437	arg1	adhesion					439:446	cell adhesion	434:446	cell adhesion required for virulence	434:469	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	5	59	theme	female	1073:1078	arg1	tract					1093:1097	the female reproductive tract	1069:1097	the female reproductive tract	1069:1097	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	4	60	theme	dramatic	817:824	arg1	impact					826:831	a dramatic impact	815:831	a dramatic impact	815:831	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	2	61	theme	cell	207:210	arg1	layer					217:221	The inner cell wall layer	197:221	The inner cell wall layer	197:221	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	2	62	theme	wall	212:215	arg1	layer					217:221	The inner cell wall layer	197:221	The inner cell wall layer	197:221	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	1	63	theme	Candida	108:114	arg1	albicans					116:123	Candida albicans	108:123	Candida albicans	108:123	The cell wall of Candida albicans is a multilayered structure consisting of polysaccharides and proteins.
36008675	5	64	theme	immune	1196:1201	arg1	response					1203:1210	a strong pro-inflammatory innate immune response	1163:1210	a strong pro-inflammatory innate immune response	1163:1210	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	3	65	theme	external	574:581	arg1	environment					583:593	the external environment	570:593	the external environment	570:593	However, the cell wall is not a static structure but is constantly being remodeled in response to the external environment.
36008675	2	66	theme	inner	201:205	arg1	layer					217:221	The inner cell wall layer	197:221	The inner cell wall layer	197:221	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	5	67	theme	cell	943:946	arg1	PAMPs					953:957	its major cell wall PAMPs	933:957	its major cell wall PAMPs from the innate immune system	933:987	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	1	68	theme	albicans	116:123	arg1	structure					143:151	a multilayered structure	128:151	a multilayered structure consisting of polysaccharides and proteins	128:194	The cell wall of Candida albicans is a multilayered structure consisting of polysaccharides and proteins.
36008675	1	68	theme	albicans	116:123	arg1	wall					100:103	The cell wall	91:103	The cell wall of Candida albicans	91:123	The cell wall of Candida albicans is a multilayered structure consisting of polysaccharides and proteins.
36008675	5	69	from	growth	887:892	arg1	media					906:910	standard media	897:910	standard media	897:910	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	5	70	theme	fungal	1132:1137	arg1	surface					1144:1150	the fungal cell surface	1128:1150	the fungal cell surface	1128:1150	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	5	71	theme	innate	968:973	arg1	system					982:987	the innate immune system	964:987	the innate immune system	964:987	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	2	72	dep	shape	303:307	arg1	the					291:293	the	291:293	the	291:293	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	0	73	theme	Wall	71:74	arg1	Exposure					81:88	Cell Wall PAMP Exposure	66:88	Cell Wall PAMP Exposure	66:88	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	5	74	theme	immune	975:980	arg1	system					982:987	the innate immune system	964:987	the innate immune system	964:987	For example, during growth in standard media, C. albicans shields its major cell wall PAMPs from the innate immune system, but during growth in acidic environments as encountered during colonization of the female reproductive tract, key PAMPs become exposed on the fungal cell surface initiating a strong pro-inflammatory innate immune response.
36008675	6	75	theme	research	1382:1389	arg1	impact					1303:1308	the subsequent impact	1288:1308	the subsequent impact this has on the host-pathogen interaction	1288:1350	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	75	theme	research	1382:1389	arg1	subject					1366:1372	the subject	1362:1372	the subject of much research	1362:1389	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	6	75	theme	research	1382:1389	arg1	impact					1217:1222	The impact	1213:1222	The impact of environmental adaptation on fungal cell wall remodeling	1213:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	0	76	theme	Cell	66:69	arg1	Exposure					81:88	Cell Wall PAMP Exposure	66:88	Cell Wall PAMP Exposure	66:88	Molecular and Microscopic Methods of Quantifying Candida albicans Cell Wall PAMP Exposure.
36008675	6	77	from	impact	1303:1308	arg1	remodeling					1272:1281	fungal cell wall remodeling	1255:1281	fungal cell wall remodeling	1255:1281	The impact of environmental adaptation on fungal cell wall remodeling, and the subsequent impact this has on the host-pathogen interaction, has been the subject of much research.
36008675	2	78	theme	functional	408:417	arg1	adhesion					439:446	cell adhesion	434:446	cell adhesion required for virulence	434:469	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	2	78	theme	functional	408:417	arg1	traits					419:424	key functional traits	404:424	key functional traits such as cell adhesion required for virulence	404:469	The inner cell wall layer is comprised of chitin and β1-3 and β1-6-glucan which contribute to the overall shape and structure of the cell, while the outer layer of highly glycosylated mannoproteins provides key functional traits such as cell adhesion required for virulence.
36008675	1	79	theme	multilayered	130:141	arg1	structure					143:151	a multilayered structure	128:151	a multilayered structure consisting of polysaccharides and proteins	128:194	The cell wall of Candida albicans is a multilayered structure consisting of polysaccharides and proteins.
36008675	1	79	theme	multilayered	130:141	arg1	wall					100:103	The cell wall	91:103	The cell wall of Candida albicans	91:123	The cell wall of Candida albicans is a multilayered structure consisting of polysaccharides and proteins.
36008675	4	80	contain	have	810:813	arg1	remodeling					778:787	remodeling	778:787	remodeling of the cell wall	778:804	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
36008675	4	80	contain	have	810:813	arg2	impact					826:831	a dramatic impact	815:831	a dramatic impact	815:831	Given that all of the cell wall components act as pathogen-associated molecular patterns (PAMPs) that are recognized by a variety of receptors on the surface of innate immune cells, remodeling of the cell wall can have a dramatic impact on the host-pathogen interaction.
35889786	5	0	theme	IL-6	1428:1431	arg1	levels					1433:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	3	1	theme	peroxidase	862:871	arg1	activities					937:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities	820:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05)	820:960	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	2	dep	cytokines	641:649	arg1	including					652:660	including	652:660	including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)	652:719	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	0	3	theme	Signaling	96:104	arg1	Pathway					106:112	the TLR4/MyD88/NF-κB Signaling Pathway	75:112	the TLR4/MyD88/NF-κB Signaling Pathway	75:112	Inosine Pretreatment Attenuates LPS-Induced Lung Injury through Regulating the TLR4/MyD88/NF-κB Signaling Pathway In Vivo.
35889786	3	4	theme	-treated	989:996	arg1	mice					998:1001	lipopolysaccharide (LPS)-treated mice	965:1001	lipopolysaccharide (LPS)-treated mice	965:1001	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	5	theme	&lt	951:953	arg1	activities					937:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities	820:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05)	820:960	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	5	6	theme	TLR4/MyD88/NF-κB	1264:1279	arg1	pathway					1291:1297	the TLR4/MyD88/NF-κB signaling pathway	1260:1297	the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response	1260:1336	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	3	7	theme	malondialdehyde	723:737	arg1	elevation					611:619	the pulmonary elevation	597:619	the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels	597:804	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	5	8	theme	HO-1	1404:1407	arg1	levels					1433:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	2	9	theme	signaling	295:303	arg1	essential					316:324	essential	316:324	essential	316:324	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	2	9	theme	signaling	295:303	arg1	pathway					305:311	The TLR4/MyD88/NF-κB signaling pathway	274:311	The TLR4/MyD88/NF-κB signaling pathway	274:311	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	5	10	theme	inosine	1230:1236	arg1	pretreatment					1238:1249	inosine pretreatment	1230:1249	inosine pretreatment	1230:1249	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	1	11	theme	anti-inflammatory	246:262	arg1	efficacy					264:271	anti-inflammatory efficacy	246:271	anti-inflammatory efficacy	246:271	Inosine is a type of purine nucleoside, which is considered to a physiological energy source, and exerts a widely range of anti-inflammatory efficacy.
35889786	3	12	theme	elevation	611:619	arg1	levels					799:804	the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels	597:804	the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels	597:804	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	13	theme	nitric	746:751	arg1	NO					760:761	NO	760:761	NO	760:761	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	13	theme	nitric	746:751	arg1	oxide					753:757	nitric oxide	746:757	nitric oxide (NO)	746:762	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	4	14	theme	Deferribacteres	1203:1217	arg1	abundance					1174:1182	the relative abundance	1161:1182	the relative abundance of Tenericutes and Deferribacteres	1161:1217	Simultaneously, inosine pre-intervention shifted the composition of the intestinal microbiota by decreasing the ratio of Firmicutes/Bacteroidetes, elevating the relative abundance of Tenericutes and Deferribacteres.
35889786	2	15	theme	TLR4/MyD88/NF-κB	278:293	arg1	essential					316:324	essential	316:324	essential	316:324	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	2	15	theme	TLR4/MyD88/NF-κB	278:293	arg1	pathway					305:311	The TLR4/MyD88/NF-κB signaling pathway	274:311	The TLR4/MyD88/NF-κB signaling pathway	274:311	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	5	16	theme	pulmonary	1306:1314	arg1	response					1329:1336	the pulmonary inflammatory response	1302:1336	the pulmonary inflammatory response	1302:1336	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	1	17	theme	efficacy	264:271	arg1	range					237:241	a widely range	228:241	a widely range of anti-inflammatory efficacy	228:271	Inosine is a type of purine nucleoside, which is considered to a physiological energy source, and exerts a widely range of anti-inflammatory efficacy.
35889786	3	18	theme	oxide	753:757	arg1	elevation					611:619	the pulmonary elevation	597:619	the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels	597:804	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	19	theme	p	949:949	arg1	&lt					951:953	p &lt	949:953	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05)	820:960	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	20	theme	inosine	547:553	arg1	pre-intervention					555:570	inosine pre-intervention	547:570	inosine pre-intervention	547:570	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	5	21	theme	inflammatory	1316:1327	arg1	response					1329:1336	the pulmonary inflammatory response	1302:1336	the pulmonary inflammatory response	1302:1336	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	6	22	theme	oral	1469:1472	arg1	administration					1474:1487	oral administration	1469:1487	oral administration of inosine pretreatment	1469:1511	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	5	23	theme	IL-1β	1417:1421	arg1	levels					1433:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	4	24	theme	intestinal	1076:1085	arg1	microbiota					1087:1096	the intestinal microbiota	1072:1096	the intestinal microbiota	1072:1096	Simultaneously, inosine pre-intervention shifted the composition of the intestinal microbiota by decreasing the ratio of Firmicutes/Bacteroidetes, elevating the relative abundance of Tenericutes and Deferribacteres.
35889786	2	25	theme	promising	400:408	arg1	target					410:415	a promising target	398:415	a promising target for host-directed strategies to improve some forms of disease-related inflammation	398:498	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	3	26	theme	lipopolysaccharide	965:982	arg1	mice					998:1001	lipopolysaccharide (LPS)-treated mice	965:1001	lipopolysaccharide (LPS)-treated mice	965:1001	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	2	27	dep	host	341:344	arg1	stresses					356:363	oxidative stresses	346:363	oxidative stresses	346:363	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	6	28	theme	microbiota	1649:1658	arg1	disorder					1660:1667	intestinal microbiota disorder	1638:1667	intestinal microbiota disorder	1638:1667	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	0	29	theme	Inosine	0:6	arg1	Pretreatment					8:19	Inosine Pretreatment	0:19	Inosine Pretreatment	0:19	Inosine Pretreatment Attenuates LPS-Induced Lung Injury through Regulating the TLR4/MyD88/NF-κB Signaling Pathway In Vivo.
35889786	6	30	theme	signaling	1603:1611	arg1	pathway					1613:1619	the TLR4/MyD88/NF-κB signaling pathway	1582:1619	the TLR4/MyD88/NF-κB signaling pathway	1582:1619	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	5	31	theme	signaling	1281:1289	arg1	pathway					1291:1297	the TLR4/MyD88/NF-κB signaling pathway	1260:1297	the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response	1260:1336	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	5	32	theme	levels	1433:1438	arg1	expression					1362:1371	the expression	1358:1371	the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1358:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	0	33	theme	LPS-Induced	32:42	arg1	Injury					49:54	LPS-Induced Lung Injury	32:54	LPS-Induced Lung Injury	32:54	Inosine Pretreatment Attenuates LPS-Induced Lung Injury through Regulating the TLR4/MyD88/NF-κB Signaling Pathway In Vivo.
35889786	5	34	theme	Nrf2	1398:1401	arg1	levels					1433:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	4	35	theme	relative	1165:1172	arg1	abundance					1174:1182	the relative abundance	1161:1182	the relative abundance of Tenericutes and Deferribacteres	1161:1217	Simultaneously, inosine pre-intervention shifted the composition of the intestinal microbiota by decreasing the ratio of Firmicutes/Bacteroidetes, elevating the relative abundance of Tenericutes and Deferribacteres.
35889786	4	36	theme	inosine	1020:1026	arg1	pre-intervention					1028:1043	inosine pre-intervention	1020:1043	inosine pre-intervention	1020:1043	Simultaneously, inosine pre-intervention shifted the composition of the intestinal microbiota by decreasing the ratio of Firmicutes/Bacteroidetes, elevating the relative abundance of Tenericutes and Deferribacteres.
35889786	1	37	theme	physiological	188:200	arg1	source					209:214	a physiological energy source	186:214	a physiological energy source	186:214	Inosine is a type of purine nucleoside, which is considered to a physiological energy source, and exerts a widely range of anti-inflammatory efficacy.
35889786	6	38	theme	pulmonary	1536:1544	arg1	response					1559:1566	LPS-induced pulmonary inflammatory response	1524:1566	LPS-induced pulmonary inflammatory response	1524:1566	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	3	39	theme	pulmonary	601:609	arg1	elevation					611:619	the pulmonary elevation	597:619	the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels	597:804	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	40	theme	superoxide	883:892	arg1	dismutase					894:902	superoxide dismutase	883:902	superoxide dismutase (SOD)	883:908	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	40	theme	superoxide	883:892	arg1	SOD					905:907	SOD	905:907	SOD	905:907	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	1	41	theme	energy	202:207	arg1	source					209:214	a physiological energy source	186:214	a physiological energy source	186:214	Inosine is a type of purine nucleoside, which is considered to a physiological energy source, and exerts a widely range of anti-inflammatory efficacy.
35889786	6	42	theme	inosine	1492:1498	arg1	pretreatment					1500:1511	inosine pretreatment	1492:1511	inosine pretreatment	1492:1511	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	3	43	theme	dismutase	894:902	arg1	activities					937:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities	820:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05)	820:960	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	2	44	theme	host-directed	421:433	arg1	strategies					435:444	host-directed strategies to improve some forms of disease-related inflammation	421:498	host-directed strategies to improve some forms of disease-related inflammation	421:498	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	0	45	theme	Lung	44:47	arg1	Injury					49:54	LPS-Induced Lung Injury	32:54	LPS-Induced Lung Injury	32:54	Inosine Pretreatment Attenuates LPS-Induced Lung Injury through Regulating the TLR4/MyD88/NF-κB Signaling Pathway In Vivo.
35889786	3	46	theme	oxygen	778:783	arg1	ROS					794:796	ROS	794:796	ROS	794:796	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	46	theme	oxygen	778:783	arg1	species					785:791	reactive oxygen species	769:791	reactive oxygen species (ROS)	769:797	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	5	47	theme	TNF-α	1410:1414	arg1	levels					1433:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	5	48	theme	COX2	1392:1395	arg1	levels					1433:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	2	49	theme	inflammation	487:498	arg1	forms					462:466	some forms	457:466	some forms of disease-related inflammation	457:498	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	3	50	theme	species	785:791	arg1	elevation					611:619	the pulmonary elevation	597:619	the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels	597:804	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	51	theme	pro-inflammatory	624:639	arg1	cytokines					641:649	pro-inflammatory cytokines	624:649	pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β))	624:720	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	52	theme	myeloperoxidase	915:929	arg1	activities					937:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities	820:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05)	820:960	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	2	53	theme	disease-related	471:485	arg1	inflammation					487:498	disease-related inflammation	471:498	disease-related inflammation	471:498	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	6	54	theme	pretreatment	1500:1511	arg1	administration					1474:1487	oral administration	1469:1487	oral administration of inosine pretreatment	1469:1511	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	4	55	theme	Tenericutes	1187:1197	arg1	abundance					1174:1182	the relative abundance	1161:1182	the relative abundance of Tenericutes and Deferribacteres	1161:1217	Simultaneously, inosine pre-intervention shifted the composition of the intestinal microbiota by decreasing the ratio of Firmicutes/Bacteroidetes, elevating the relative abundance of Tenericutes and Deferribacteres.
35889786	3	56	theme	cytokines	641:649	arg1	elevation					611:619	the pulmonary elevation	597:619	the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels	597:804	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	5	57	theme	pulmonary	1376:1384	arg1	iNOS					1386:1389	pulmonary iNOS	1376:1389	pulmonary iNOS	1376:1389	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	2	58	theme	oxidative	346:354	arg1	stresses					356:363	oxidative stresses	346:363	oxidative stresses	346:363	The TLR4/MyD88/NF-κB signaling pathway is essential for preventing host oxidative stresses and inflammation, and represents a promising target for host-directed strategies to improve some forms of disease-related inflammation.
35889786	5	59	from	pathway	1291:1297	arg1	response					1329:1336	the pulmonary inflammatory response	1302:1336	the pulmonary inflammatory response	1302:1336	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	3	60	theme	reactive	769:776	arg1	ROS					794:796	ROS	794:796	ROS	794:796	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	60	theme	reactive	769:776	arg1	species					785:791	reactive oxygen species	769:791	reactive oxygen species (ROS)	769:797	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	4	61	theme	Firmicutes/Bacteroidetes	1125:1148	arg1	ratio					1116:1120	the ratio	1112:1120	the ratio of Firmicutes/Bacteroidetes	1112:1148	Simultaneously, inosine pre-intervention shifted the composition of the intestinal microbiota by decreasing the ratio of Firmicutes/Bacteroidetes, elevating the relative abundance of Tenericutes and Deferribacteres.
35889786	5	62	theme	iNOS	1386:1389	arg1	levels					1433:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels	1376:1438	Moreover, inosine pretreatment affected the TLR4/MyD88/NF-κB signaling pathway in the pulmonary inflammatory response, and then regulated the expression of pulmonary iNOS, COX2, Nrf2, HO-1, TNF-α, IL-1β, and IL-6 levels.
35889786	6	63	theme	TLR4/MyD88/NF-κB	1586:1601	arg1	pathway					1613:1619	the TLR4/MyD88/NF-κB signaling pathway	1582:1619	the TLR4/MyD88/NF-κB signaling pathway	1582:1619	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	6	64	theme	inflammatory	1546:1557	arg1	response					1559:1566	LPS-induced pulmonary inflammatory response	1524:1566	LPS-induced pulmonary inflammatory response	1524:1566	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	3	65	theme	present	508:514	arg1	study					516:520	the present study	504:520	the present study	504:520	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	66	theme	pulmonary	824:832	arg1	MPO					932:934	MPO	932:934	MPO	932:934	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	66	theme	pulmonary	824:832	arg1	CAT					844:846	CAT	844:846	CAT	844:846	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	66	theme	pulmonary	824:832	arg1	catalase					834:841	the pulmonary catalase	820:841	the pulmonary catalase (CAT)	820:847	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	0	67	theme	TLR4/MyD88/NF-κB	79:94	arg1	Pathway					106:112	the TLR4/MyD88/NF-κB Signaling Pathway	75:112	the TLR4/MyD88/NF-κB Signaling Pathway	75:112	Inosine Pretreatment Attenuates LPS-Induced Lung Injury through Regulating the TLR4/MyD88/NF-κB Signaling Pathway In Vivo.
35889786	3	68	theme	tumor	662:666	arg1	IL-1β					714:718	IL-1β	714:718	IL-1β	714:718	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	68	theme	tumor	662:666	arg1	TNF-α					687:691	TNF-α	687:691	TNF-α	687:691	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	68	theme	tumor	662:666	arg1	factor-α					677:684	tumor necrosis factor-α	662:684	tumor necrosis factor-α (TNF-α)	662:692	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	4	69	theme	microbiota	1087:1096	arg1	composition					1057:1067	the composition	1053:1067	the composition of the intestinal microbiota	1053:1096	Simultaneously, inosine pre-intervention shifted the composition of the intestinal microbiota by decreasing the ratio of Firmicutes/Bacteroidetes, elevating the relative abundance of Tenericutes and Deferribacteres.
35889786	1	70	theme	purine	144:149	arg1	nucleoside					151:160	purine nucleoside	144:160	purine nucleoside	144:160	Inosine is a type of purine nucleoside, which is considered to a physiological energy source, and exerts a widely range of anti-inflammatory efficacy.
35889786	3	71	theme	catalase	834:841	arg1	activities					937:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities	820:946	the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05)	820:960	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	6	72	theme	intestinal	1638:1647	arg1	disorder					1660:1667	intestinal microbiota disorder	1638:1667	intestinal microbiota disorder	1638:1667	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	3	73	theme	necrosis	668:675	arg1	IL-1β					714:718	IL-1β	714:718	IL-1β	714:718	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	73	theme	necrosis	668:675	arg1	TNF-α					687:691	TNF-α	687:691	TNF-α	687:691	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	73	theme	necrosis	668:675	arg1	factor-α					677:684	tumor necrosis factor-α	662:684	tumor necrosis factor-α (TNF-α)	662:692	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	6	74	theme	LPS-induced	1524:1534	arg1	response					1559:1566	LPS-induced pulmonary inflammatory response	1524:1566	LPS-induced pulmonary inflammatory response	1524:1566	These findings suggest that oral administration of inosine pretreatment attenuates LPS-induced pulmonary inflammatory response by regulating the TLR4/MyD88/NF-κB signaling pathway, and ameliorates intestinal microbiota disorder.
35889786	1	75	theme	nucleoside	151:160	arg1	type					136:139	a type	134:139	a type	134:139	Inosine is a type of purine nucleoside, which is considered to a physiological energy source, and exerts a widely range of anti-inflammatory efficacy.
35889786	1	75	theme	nucleoside	151:160	arg1	Inosine					123:129	Inosine	123:129	Inosine	123:129	Inosine is a type of purine nucleoside, which is considered to a physiological energy source, and exerts a widely range of anti-inflammatory efficacy.
35889786	3	76	theme	glutathione	850:860	arg1	GSH-Px					874:879	GSH-Px	874:879	GSH-Px	874:879	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
35889786	3	76	theme	glutathione	850:860	arg1	peroxidase					862:871	glutathione peroxidase	850:871	glutathione peroxidase (GSH-Px)	850:880	In the present study, the results showed that inosine pre-intervention significantly suppressed the pulmonary elevation of pro-inflammatory cytokines (including tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), malondialdehyde (MDA), nitric oxide (NO), and reactive oxygen species (ROS) levels, and restored the pulmonary catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and myeloperoxidase (MPO) activities (p &lt; 0.05) in lipopolysaccharide (LPS)-treated mice.
36899786	9	0	theme	Beta	1405:1408	arg1	diversity					1410:1418	Beta diversity	1405:1418	Beta diversity	1405:1418	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	2	1	theme	red	295:297	arg1	deer					299:302	red deer	295:302	red deer (Cervus elaphus)	295:319	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	11	2	theme	secondary	1681:1689	arg1	pathway					1691:1697	the secondary pathway	1677:1697	the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids	1677:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	7	3	theme	red	1223:1225	arg1	deer					1227:1230	the wild and captive red deer	1202:1230	the wild and captive red deer	1202:1230	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	11	4	theme	metabolism	1757:1766	arg1	pathway					1691:1697	the secondary pathway	1677:1697	the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids	1677:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	9	5	theme	inter-group	1438:1448	arg1	differences					1450:1460	significant inter-group differences	1426:1460	significant inter-group differences between wild and captive deer (p < 0.05)	1426:1501	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	4	6	theme	Fecal	571:575	arg1	composition					588:598	Fecal microbiota composition	571:598	Fecal microbiota composition	571:598	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	8	7	from	females	1372:1378	arg1	deer					1388:1391	wild deer	1383:1391	wild deer (p < 0.05)	1383:1402	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	8	7	from	females	1372:1378	arg1	<					1396:1396	p < 0.05	1394:1401	p < 0.05	1394:1401	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	5	8	theme	potential	778:786	arg1	information					810:820	potential function distribution information	778:820	potential function distribution information	778:820	Based on Picrust2 prediction software, potential function distribution information was evaluated by comparing the Kyoto Encyclopedia of Genes and Genome (KEGG).
36899786	4	9	theme	composition	588:598	arg1	analyses					614:621	Fecal microbiota composition and diversity analyses	571:621	Fecal microbiota composition and diversity analyses	571:621	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	9	10	from	deer	1577:1580	arg1	female					1542:1547	female	1542:1547	female	1542:1547	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	8	11	from	males	1362:1366	arg1	deer					1388:1391	wild deer	1383:1391	wild deer (p < 0.05)	1383:1402	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	8	11	from	males	1362:1366	arg1	<					1396:1396	p < 0.05	1394:1401	p < 0.05	1394:1401	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	3	12	theme	fecal	488:492	arg1	samples					494:500	fecal samples	488:500	fecal samples from both wild and captive red deer	488:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	5	13	theme	distribution	797:808	arg1	information					810:820	potential function distribution information	778:820	potential function distribution information	778:820	Based on Picrust2 prediction software, potential function distribution information was evaluated by comparing the Kyoto Encyclopedia of Genes and Genome (KEGG).
36899786	11	14	theme	amino	1797:1801	arg1	acids					1803:1807	other amino acids	1791:1807	other amino acids	1791:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	7	15	theme	microbiota	1188:1197	arg1	species					1171:1177	The dominant species	1158:1177	The dominant species of fecal microbiota in the wild and captive red deer	1158:1230	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	7	15	theme	microbiota	1188:1197	arg1	similar					1237:1243	similar	1237:1243	similar	1237:1243	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	11	16	from	pathway	1691:1697	arg1	different					1828:1836	different	1828:1836	different	1828:1836	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	6	17	theme	higher	1126:1131	arg1	number					1133:1138	a significantly higher number	1110:1138	a significantly higher number of Bacteroidetes	1110:1155	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	3	18	theme	molecular	426:434	arg1	techniques					443:452	non-invasive molecular sexing techniques	413:452	non-invasive molecular sexing techniques	413:452	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	12	19	from	helpful	1940:1946	arg1	summary					1842:1848	summary	1842:1848	summary	1842:1848	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	5	20	theme	Picrust2	748:755	arg1	software					768:775	Picrust2 prediction software	748:775	Picrust2 prediction software	748:775	Based on Picrust2 prediction software, potential function distribution information was evaluated by comparing the Kyoto Encyclopedia of Genes and Genome (KEGG).
36899786	12	21	theme	conservation	2097:2108	arg1	applications					2055:2066	future applications	2048:2066	future applications of population management and conservation	2048:2108	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	3	22	theme	wild	512:515	arg1	deer					533:536	both wild and captive red deer	507:536	both wild and captive red deer	507:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	9	23	theme	p	1493:1493	arg1	deer					1487:1490	wild and captive deer	1470:1490	wild and captive deer (p < 0.05)	1470:1501	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	9	23	theme	p	1493:1493	arg1	<					1495:1495	p < 0.05	1493:1500	p < 0.05	1493:1500	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	0	24	from	Sex	58:60	arg1	Deer					69:72	Red Deer	65:72	Red Deer (Cervus elaphus)	65:89	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	7	25	theme	dominant	1162:1169	arg1	species					1171:1177	The dominant species	1158:1177	The dominant species of fecal microbiota in the wild and captive red deer	1158:1230	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	7	25	theme	dominant	1162:1169	arg1	similar					1237:1243	similar	1237:1243	similar	1237:1243	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	2	26	dep	structure	249:257	arg1	the					245:247	the	245:247	the	245:247	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	1	27	theme	Gut	92:94	arg1	microbiota					96:105	Gut microbiota	92:105	Gut microbiota	92:105	Gut microbiota play an important role in impacting the host's metabolism, immunity, speciation, and many other functions.
36899786	11	28	theme	biosynthesis	1721:1732	arg1	pathway					1691:1697	the secondary pathway	1677:1697	the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids	1677:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	3	29	from	sex	481:483	arg1	deer					533:536	both wild and captive red deer	507:536	both wild and captive red deer	507:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	6	30	theme	=	1101:1101	arg1	n					1099:1099	n = 3	1099:1103	n = 3	1099:1103	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	4	31	theme	diversity	604:612	arg1	analyses					614:621	Fecal microbiota composition and diversity analyses	571:621	Fecal microbiota composition and diversity analyses	571:621	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	11	32	from	different	1828:1836	arg1	pathway					1691:1697	the secondary pathway	1677:1697	the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids	1677:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	3	33	theme	captive	521:527	arg1	deer					533:536	both wild and captive red deer	507:536	both wild and captive red deer	507:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	1	34	theme	important	115:123	arg1	role					125:128	an important role	112:128	an important role	112:128	Gut microbiota play an important role in impacting the host's metabolism, immunity, speciation, and many other functions.
36899786	9	35	theme	significant	1510:1520	arg1	differences					1522:1532	no significant differences	1507:1532	no significant differences between female and male in wild or captive deer	1507:1580	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	8	36	theme	alpha	1269:1273	arg1	index					1285:1289	The alpha diversity index	1265:1289	The alpha diversity index	1265:1289	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	6	37	dep	CF	1084:1085	arg1	CM					1095:1096	CM	1095:1096	CM	1095:1096	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	37	dep	CF	1084:1085	arg1	n					1088:1088	n = 8	1088:1092	n = 8	1088:1092	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	37	dep	CF	1084:1085	arg1	n					1099:1099	n = 3	1099:1103	n = 3	1099:1103	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	0	38	theme	Red	65:67	arg1	Deer					69:72	Red Deer	65:72	Red Deer (Cervus elaphus)	65:89	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	12	39	from	variations	1886:1895	arg1	microbiota					1910:1919	the fecal microbiota	1900:1919	the fecal microbiota of red deer	1900:1931	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	10	40	theme	important	1611:1619	arg1	pathway					1621:1627	the most important pathway	1602:1627	the most important pathway	1602:1627	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	10	40	theme	important	1611:1619	arg1	metabolism					1587:1596	The metabolism	1583:1596	The metabolism	1583:1596	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	0	41	from	Alterations	0:10	arg1	Microbiota					21:30	Fecal Microbiota	15:30	Fecal Microbiota	15:30	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	2	42	dep	deer	299:302	arg1	elaphus					312:318	Cervus elaphus	305:318	Cervus elaphus	305:318	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	0	43	from	Environment	42:52	arg1	Deer					69:72	Red Deer	65:72	Red Deer (Cervus elaphus)	65:89	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	4	44	theme	gene	692:695	arg1	region					669:674	the V4-V5 region	659:674	the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform	659:736	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	4	45	theme	V4-V5	663:667	arg1	region					669:674	the V4-V5 region	659:674	the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform	659:736	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	3	46	from	deer	533:536	arg1	sex					481:483	the sex	477:483	the sex of fecal samples from both wild and captive red deer	477:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	3	46	from	deer	533:536	arg1	samples					494:500	fecal samples	488:500	fecal samples from both wild and captive red deer	488:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	12	47	from	summary	1842:1848	arg1	helpful					1940:1946	helpful	1940:1946	helpful	1940:1946	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	8	48	theme	microbiota	1329:1338	arg1	diversity					1340:1348	fecal microbiota diversity	1323:1348	fecal microbiota diversity	1323:1348	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	6	49	dep	=	969:969	arg1	10					971:972	10	971:972	10	971:972	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	0	50	theme	Fecal	15:19	arg1	Microbiota					21:30	Fecal Microbiota	15:30	Fecal Microbiota	15:30	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	6	51	theme	deer	957:960	arg1	microbiota					934:943	the fecal microbiota	924:943	the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12)	924:985	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	12	52	theme	red	1924:1926	arg1	deer					1928:1931	red deer	1924:1931	red deer	1924:1931	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	3	53	used	used	459:462	arg2	techniques					443:452	non-invasive molecular sexing techniques	413:452	non-invasive molecular sexing techniques	413:452	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	2	54	theme	different	381:389	arg1	diets					391:395	different diets	381:395	different diets	381:395	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	6	55	theme	=	1090:1090	arg1	n					1088:1088	n = 8	1088:1092	n = 8	1088:1092	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	11	56	theme	glycan	1714:1719	arg1	biosynthesis					1721:1732	glycan biosynthesis	1714:1732	glycan biosynthesis	1714:1732	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	2	57	theme	fecal	275:279	arg1	microbiota					281:290	fecal microbiota	275:290	fecal microbiota	275:290	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	8	58	theme	wild	1383:1386	arg1	deer					1388:1391	wild deer	1383:1391	wild deer (p < 0.05)	1383:1402	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	8	58	theme	wild	1383:1386	arg1	<					1396:1396	p < 0.05	1394:1401	p < 0.05	1394:1401	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	4	59	theme	Illumina	714:721	arg1	platform					729:736	the Illumina HiSeq platform	710:736	the Illumina HiSeq platform	710:736	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	3	60	theme	sexing	436:441	arg1	techniques					443:452	non-invasive molecular sexing techniques	413:452	non-invasive molecular sexing techniques	413:452	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	12	61	dep	population	2071:2080	arg1	management					2082:2091	management	2082:2091	management	2082:2091	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	10	62	theme	KEGG	1651:1654	arg1	analysis					1664:1671	KEGG pathway analysis	1651:1671	KEGG pathway analysis	1651:1671	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	3	63	theme	non-invasive	413:424	arg1	techniques					443:452	non-invasive molecular sexing techniques	413:452	non-invasive molecular sexing techniques	413:452	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	1	64	theme	other	197:201	arg1	functions					203:211	many other functions	192:211	many other functions	192:211	Gut microbiota play an important role in impacting the host's metabolism, immunity, speciation, and many other functions.
36899786	7	65	theme	genus	1252:1256	arg1	level					1258:1262	the genus level	1248:1262	the genus level	1248:1262	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	11	66	theme	energy	1750:1755	arg1	metabolism					1757:1766	energy metabolism	1750:1766	energy metabolism	1750:1766	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	9	67	theme	significant	1426:1436	arg1	differences					1450:1460	significant inter-group differences	1426:1460	significant inter-group differences between wild and captive deer (p < 0.05)	1426:1501	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	0	68	attach	Linked	32:37	arg2	Alterations					0:10	Alterations	0:10	Alterations in Fecal Microbiota	0:30	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	0	68	attach	Linked	32:37	arg1	Sex					58:60	Sex	58:60	Sex	58:60	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	0	68	attach	Linked	32:37	arg1	Environment					42:52	Environment	42:52	Environment	42:52	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	7	69	theme	captive	1215:1221	arg1	deer					1227:1230	the wild and captive red deer	1202:1230	the wild and captive red deer	1202:1230	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	12	70	theme	compositional	1857:1869	arg1	variations					1886:1895	these compositional and functional variations	1851:1895	these compositional and functional variations in the fecal microbiota of red deer	1851:1931	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	10	71	theme	analysis	1664:1671	arg1	level					1642:1646	the first level	1632:1646	the first level of KEGG pathway analysis	1632:1671	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	5	72	theme	function	788:795	arg1	information					810:820	potential function distribution information	778:820	potential function distribution information	778:820	Based on Picrust2 prediction software, potential function distribution information was evaluated by comparing the Kyoto Encyclopedia of Genes and Genome (KEGG).
36899786	10	73	from	level	1642:1646	arg1	pathway					1621:1627	the most important pathway	1602:1627	the most important pathway	1602:1627	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	10	73	from	level	1642:1646	arg1	metabolism					1587:1596	The metabolism	1583:1596	The metabolism	1583:1596	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	9	74	theme	wild	1470:1473	arg1	deer					1487:1490	wild and captive deer	1470:1490	wild and captive deer (p < 0.05)	1470:1501	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	9	74	theme	wild	1470:1473	arg1	<					1495:1495	p < 0.05	1493:1500	p < 0.05	1493:1500	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	7	75	theme	wild	1206:1209	arg1	deer					1227:1230	the wild and captive red deer	1202:1230	the wild and captive red deer	1202:1230	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	4	76	theme	microbiota	577:586	arg1	composition					588:598	Fecal microbiota composition	571:598	Fecal microbiota composition	571:598	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	11	77	theme	metabolism	1777:1786	arg1	pathway					1691:1697	the secondary pathway	1677:1697	the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids	1677:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	9	78	theme	captive	1479:1485	arg1	deer					1487:1490	wild and captive deer	1470:1490	wild and captive deer (p < 0.05)	1470:1501	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	9	78	theme	captive	1479:1485	arg1	<					1495:1495	p < 0.05	1493:1500	p < 0.05	1493:1500	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	12	79	theme	functional	1875:1884	arg1	variations					1886:1895	these compositional and functional variations	1851:1895	these compositional and functional variations in the fecal microbiota of red deer	1851:1931	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	5	80	theme	prediction	757:766	arg1	software					768:775	Picrust2 prediction software	748:775	Picrust2 prediction software	748:775	Based on Picrust2 prediction software, potential function distribution information was evaluated by comparing the Kyoto Encyclopedia of Genes and Genome (KEGG).
36899786	7	81	from	level	1258:1262	arg1	species					1171:1177	The dominant species	1158:1177	The dominant species of fecal microbiota in the wild and captive red deer	1158:1230	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	7	81	from	level	1258:1262	arg1	similar					1237:1243	similar	1237:1243	similar	1237:1243	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	11	82	theme	other	1791:1795	arg1	acids					1803:1807	other amino acids	1791:1807	other amino acids	1791:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	7	83	theme	fecal	1182:1186	arg1	microbiota					1188:1197	fecal microbiota	1182:1197	fecal microbiota	1182:1197	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	8	84	dep	males	1362:1366	arg1	the					1358:1360	the	1358:1360	the	1358:1360	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	3	85	theme	samples	494:500	arg1	sex					481:483	the sex	477:483	the sex of fecal samples from both wild and captive red deer	477:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	12	86	theme	future	2048:2053	arg1	applications					2055:2066	future applications	2048:2066	future applications of population management and conservation	2048:2108	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	12	87	theme	population	2071:2080	arg1	applications					2055:2066	future applications	2048:2066	future applications of population management and conservation	2048:2108	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	6	88	theme	captive	1070:1076	arg1	deer					1078:1081	the captive deer	1066:1081	the captive deer (CF, n = 8; CM, n = 3)	1066:1104	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	88	theme	captive	1070:1076	arg1	CF					1084:1085	CF	1084:1085	CF	1084:1085	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	3	89	theme	red	529:531	arg1	deer					533:536	both wild and captive red deer	507:536	both wild and captive red deer	507:536	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	4	90	theme	16S	683:685	arg1	gene					692:695	the 16S rRNA gene	679:695	the 16S rRNA gene	679:695	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	6	91	theme	fecal	928:932	arg1	microbiota					934:943	the fecal microbiota	924:943	the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12)	924:985	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	8	92	theme	diversity	1275:1283	arg1	index					1285:1289	The alpha diversity index	1265:1289	The alpha diversity index	1265:1289	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	11	93	theme	acids	1803:1807	arg1	biosynthesis					1721:1732	glycan biosynthesis	1714:1732	glycan biosynthesis	1714:1732	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	11	93	theme	acids	1803:1807	arg1	metabolism					1738:1747	metabolism	1738:1747	metabolism	1738:1747	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	11	93	theme	acids	1803:1807	arg1	metabolism					1702:1711	metabolism	1702:1711	metabolism	1702:1711	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	11	93	theme	acids	1803:1807	arg1	metabolism					1757:1766	energy metabolism	1750:1766	energy metabolism	1750:1766	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	11	93	theme	acids	1803:1807	arg1	metabolism					1777:1786	the metabolism	1773:1786	the metabolism of other amino acids	1773:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	6	94	contain	had	1106:1108	arg2	number					1133:1138	a significantly higher number	1110:1138	a significantly higher number of Bacteroidetes	1110:1155	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	94	contain	had	1106:1108	arg1	deer					1078:1081	the captive deer	1066:1081	the captive deer (CF, n = 8; CM, n = 3)	1066:1104	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	94	contain	had	1106:1108	arg1	CF					1084:1085	CF	1084:1085	CF	1084:1085	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	12	95	theme	conservation	1960:1971	arg1	management					1973:1982	conservation management	1960:1982	conservation management	1960:1982	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	6	96	theme	Bacteroidetes	1143:1155	arg1	number					1133:1138	a significantly higher number	1110:1138	a significantly higher number of Bacteroidetes	1110:1155	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	97	theme	=	969:969	arg1	n					967:967	n	967:967	n = 10	967:972	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	8	98	theme	fecal	1323:1327	arg1	diversity					1340:1348	fecal microbiota diversity	1323:1348	fecal microbiota diversity	1323:1348	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	4	99	theme	rRNA	687:690	arg1	gene					692:695	the 16S rRNA gene	679:695	the 16S rRNA gene	679:695	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	4	100	from	region	669:674	arg1	amplicons					644:652	amplicons	644:652	amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform	644:736	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	0	101	dep	Deer	69:72	arg1	elaphus					82:88	Cervus elaphus	75:88	Cervus elaphus	75:88	Alterations in Fecal Microbiota Linked to Environment and Sex in Red Deer (Cervus elaphus).
36899786	8	102	theme	significant	1297:1307	arg1	difference					1309:1318	significant difference	1297:1318	significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05)	1297:1402	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	9	103	theme	wild	1561:1564	arg1	deer					1577:1580	wild or captive deer	1561:1580	wild or captive deer	1561:1580	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	6	104	dep	WF	963:964	arg1	WM					975:976	WM	975:976	WM	975:976	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	104	dep	WF	963:964	arg1	n					967:967	n	967:967	n = 10	967:972	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	104	dep	WF	963:964	arg1	n					979:979	n = 12	979:984	n = 12	979:984	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	7	105	from	species	1171:1177	arg1	deer					1227:1230	the wild and captive red deer	1202:1230	the wild and captive red deer	1202:1230	The dominant species of fecal microbiota in the wild and captive red deer were similar at the genus level.
36899786	9	106	theme	captive	1569:1575	arg1	deer					1577:1580	wild or captive deer	1561:1580	wild or captive deer	1561:1580	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	12	107	theme	policy	1988:1993	arg1	decision-making					1995:2009	policy decision-making	1988:2009	policy decision-making	1988:2009	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	3	108	theme	overwintering	549:561	arg1	period					563:568	the overwintering period	545:568	the overwintering period	545:568	In this study, non-invasive molecular sexing techniques were used to determine the sex of fecal samples from both wild and captive red deer during the overwintering period.
36899786	6	109	theme	wild	952:955	arg1	deer					957:960	the wild deer	948:960	the wild deer (WF, n = 10; WM, n = 12)	948:985	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	6	109	theme	wild	952:955	arg1	WF					963:964	WF	963:964	WF	963:964	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	2	110	theme	diets	391:395	arg1	intake					371:376	the intake	367:376	the intake of different diets	367:395	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	4	111	theme	HiSeq	723:727	arg1	platform					729:736	the Illumina HiSeq platform	710:736	the Illumina HiSeq platform	710:736	Fecal microbiota composition and diversity analyses were performed using amplicons from the V4-V5 region of the 16S rRNA gene sequenced on the Illumina HiSeq platform.
36899786	8	112	from	difference	1309:1318	arg1	diversity					1340:1348	fecal microbiota diversity	1323:1348	fecal microbiota diversity	1323:1348	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	9	113	from	female	1542:1547	arg1	deer					1577:1580	wild or captive deer	1561:1580	wild or captive deer	1561:1580	Beta diversity shows significant inter-group differences between wild and captive deer (p < 0.05) but no significant differences between female and male in wild or captive deer.
36899786	12	114	theme	important	2022:2030	arg1	information					2032:2042	important information	2022:2042	important information	2022:2042	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	10	115	theme	first	1636:1640	arg1	level					1642:1646	the first level	1632:1646	the first level of KEGG pathway analysis	1632:1671	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	12	116	theme	deer	1928:1931	arg1	microbiota					1910:1919	the fecal microbiota	1900:1919	the fecal microbiota of red deer	1900:1931	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	2	117	theme	microbiota	281:290	arg1	structure					249:257	structure	249:257	structure	249:257	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	2	117	theme	microbiota	281:290	arg1	function					263:270	function	263:270	function	263:270	How sex and environment affect the structure and function of fecal microbiota in red deer (Cervus elaphus) is still unclear, particularly with regard to the intake of different diets.
36899786	11	118	theme	metabolism	1702:1711	arg1	pathway					1691:1697	the secondary pathway	1677:1697	the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids	1677:1807	In the secondary pathway of metabolism, glycan biosynthesis and metabolism, energy metabolism, and the metabolism of other amino acids were significantly different.
36899786	6	119	theme	=	981:981	arg1	n					979:979	n = 12	979:984	n = 12	979:984	The results showed that the fecal microbiota of the wild deer (WF, n = 10; WM, n = 12) was significantly enriched in Firmicutes and decreased in Bacteroidetes, while the captive deer (CF, n = 8; CM, n = 3) had a significantly higher number of Bacteroidetes.
36899786	8	120	theme	p	1394:1394	arg1	deer					1388:1391	wild deer	1383:1391	wild deer (p < 0.05)	1383:1402	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	8	120	theme	p	1394:1394	arg1	<					1396:1396	p < 0.05	1394:1401	p < 0.05	1394:1401	The alpha diversity index shows significant difference in fecal microbiota diversity between the males and females in wild deer (p < 0.05).
36899786	10	121	theme	pathway	1656:1662	arg1	analysis					1664:1671	KEGG pathway analysis	1651:1671	KEGG pathway analysis	1651:1671	The metabolism was the most important pathway at the first level of KEGG pathway analysis.
36899786	12	122	theme	fecal	1904:1908	arg1	microbiota					1910:1919	the fecal microbiota	1900:1919	the fecal microbiota of red deer	1900:1931	In summary, these compositional and functional variations in the fecal microbiota of red deer may be helpful for guiding conservation management and policy decision-making, providing important information for future applications of population management and conservation.
36899786	1	123	theme	many	192:195	arg1	functions					203:211	many other functions	192:211	many other functions	192:211	Gut microbiota play an important role in impacting the host's metabolism, immunity, speciation, and many other functions.
37121079	9	0	link	SMOF-derived	2000:2011	arg1	LMW-DOM					2013:2019	SPOF-DOM and SMOF-derived LMW-DOM	1987:2019	SPOF-DOM and SMOF-derived LMW-DOM	1987:2019	Furthermore, the polysaccharides which preferentially participated in the binding of Pb to SPOF-DOM and SMOF-derived LMW-DOM may pose a higher risk of Pb in the environment.
37121079	2	1	theme	shrimp	551:556	arg1	SPOF					593:596	SPOF	593:596	SPOF	593:596	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	1	theme	shrimp	551:556	arg1	fertilizers					580:590	shrimp peptide-based organic fertilizers	551:590	shrimp peptide-based organic fertilizers (SPOF)	551:597	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	8	2	dep	aromatic	1828:1835	arg1	CC					1838:1839	CC	1838:1839	CC	1838:1839	In contrast, irrespective of MWs, polysaccharides and humic-like substances with aromatic (CC) groups in SPOF-DOM displayed a faster response to Pb.
37121079	1	3	theme	soil	249:252	arg1	environment					254:264	the soil environment	245:264	the soil environment	245:264	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	3	4	theme	heteroatom-containing	1031:1051	arg1	compounds					1053:1061	more microbially-transformed heteroatom-containing compounds	1002:1061	more microbially-transformed heteroatom-containing compounds	1002:1061	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	2	5	theme	two-dimensional	724:738	arg1	2D-COS					766:771	2D-COS	766:771	2D-COS	766:771	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	5	theme	two-dimensional	724:738	arg1	spectroscopy					752:763	two-dimensional correlation spectroscopy	724:763	two-dimensional correlation spectroscopy (2D-COS)	724:772	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	10	6	from	metals	2206:2211	arg1	environments					2220:2231	the environments	2216:2231	the environments	2216:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	2	7	theme	organic	572:578	arg1	SPOF					593:596	SPOF	593:596	SPOF	593:596	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	7	theme	organic	572:578	arg1	fertilizers					580:590	shrimp peptide-based organic fertilizers	551:590	shrimp peptide-based organic fertilizers (SPOF)	551:597	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	9	8	theme	Pb	1981:1982	arg1	binding					1970:1976	the binding	1966:1976	the binding of Pb to SPOF-DOM and SMOF-derived LMW-DOM	1966:2019	Furthermore, the polysaccharides which preferentially participated in the binding of Pb to SPOF-DOM and SMOF-derived LMW-DOM may pose a higher risk of Pb in the environment.
37121079	5	9	dep	obvious	1311:1317	arg1	MW-dependent					1319:1330	MW-dependent	1319:1330	MW-dependent	1319:1330	SMOF-DOM exhibited more obvious MW-dependent heterogeneity in molecular components compared to SPOF-DOM with higher molecular diversity.
37121079	8	10	with	substances	1812:1821	arg1	groups					1842:1847	aromatic (CC) groups	1828:1847	aromatic (CC) groups in SPOF-DOM	1828:1859	In contrast, irrespective of MWs, polysaccharides and humic-like substances with aromatic (CC) groups in SPOF-DOM displayed a faster response to Pb.
37121079	0	11	theme	dissolved	116:124	arg1	matter					134:139	organic fertilizers-derived dissolved organic matter	88:139	organic fertilizers-derived dissolved organic matter	88:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	6	12	from	SMOF-DOM	1481:1488	arg1	SPOF-DOM					1513:1520	SPOF-DOM	1513:1520	SPOF-DOM	1513:1520	Moreover, 2D-COS indicated phenol and carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM exhibited superior binding affinities for Pb.
37121079	8	13	theme	humic-like	1801:1810	arg1	substances					1812:1821	humic-like substances	1801:1821	humic-like substances	1801:1821	In contrast, irrespective of MWs, polysaccharides and humic-like substances with aromatic (CC) groups in SPOF-DOM displayed a faster response to Pb.
37121079	1	14	theme	dissolved	205:213	arg1	DOM					231:233	DOM	231:233	DOM	231:233	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	14	theme	dissolved	205:213	arg1	matter					223:228	dissolved organic matter	205:228	dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	205:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	4	15	theme	aromatic	1115:1122	arg1	compounds					1124:1132	more aromatic compounds	1110:1132	more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components	1110:1220	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	0	16	theme	matter	134:139	arg1	origins					77:83	two origins	73:83	two origins of organic fertilizers-derived dissolved organic matter	73:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	6	17	from	polysaccharides	1494:1508	arg1	SPOF-DOM					1513:1520	SPOF-DOM	1513:1520	SPOF-DOM	1513:1520	Moreover, 2D-COS indicated phenol and carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM exhibited superior binding affinities for Pb.
37121079	1	18	theme	matter	223:228	arg1	metals					315:320	heavy metals	309:320	heavy metals	309:320	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	18	theme	matter	223:228	arg1	amounts					194:200	large amounts	188:200	large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	188:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	18	theme	matter	223:228	arg1	DOM					231:233	DOM	231:233	DOM	231:233	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	18	theme	matter	223:228	arg1	fates					300:304	fates	300:304	fates of heavy metals	300:320	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	18	theme	matter	223:228	arg1	matter					223:228	dissolved organic matter	205:228	dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	205:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	5	19	from	heterogeneity	1332:1344	arg1	components					1359:1368	molecular components	1349:1368	molecular components	1349:1368	SMOF-DOM exhibited more obvious MW-dependent heterogeneity in molecular components compared to SPOF-DOM with higher molecular diversity.
37121079	2	20	theme	cyclotron	670:678	arg1	spectrometry					695:706	Fourier-transform ion cyclotron resonance mass spectrometry	648:706	Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)	648:718	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	20	theme	cyclotron	670:678	arg1	FT-ICR-MS					709:717	FT-ICR-MS	709:717	FT-ICR-MS	709:717	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	5	21	with	SPOF-DOM	1382:1389	arg1	diversity					1413:1421	higher molecular diversity	1396:1421	higher molecular diversity	1396:1421	SMOF-DOM exhibited more obvious MW-dependent heterogeneity in molecular components compared to SPOF-DOM with higher molecular diversity.
37121079	2	22	theme	Fourier-transform	648:664	arg1	spectrometry					695:706	Fourier-transform ion cyclotron resonance mass spectrometry	648:706	Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)	648:718	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	22	theme	Fourier-transform	648:664	arg1	FT-ICR-MS					709:717	FT-ICR-MS	709:717	FT-ICR-MS	709:717	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	4	23	theme	protein-like	1198:1209	arg1	components					1211:1220	HMW-DOM but abundant protein-like components	1177:1220	HMW-DOM but abundant protein-like components	1177:1220	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	6	24	from	groups	1471:1476	arg1	SMOF-DOM					1481:1488	SMOF-DOM	1481:1488	SMOF-DOM	1481:1488	Moreover, 2D-COS indicated phenol and carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM exhibited superior binding affinities for Pb.
37121079	6	24	from	groups	1471:1476	arg1	polysaccharides					1494:1508	polysaccharides	1494:1508	polysaccharides	1494:1508	Moreover, 2D-COS indicated phenol and carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM exhibited superior binding affinities for Pb.
37121079	7	25	theme	fulvic-like	1649:1659	arg1	substances					1661:1670	fulvic-like substances	1649:1670	fulvic-like substances	1649:1670	Pb binding to HMW-DOM derived from SMOF first occurred in the phenolic groups in fulvic-like substances, while polysaccharides in LMW-DOM first participated in the binding of Pb.
37121079	2	26	theme	lead	473:476	arg1	behaviors					491:499	lead (Pb) binding behaviors	473:499	lead (Pb) binding behaviors of DOM	473:506	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	4	27	theme	hydroxyl	1158:1165	arg1	groups					1167:1172	ample carboxyl and hydroxyl groups	1139:1172	groups	1167:1172	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	0	28	theme	binding	52:58	arg1	behaviors					60:68	(II) binding behaviors	47:68	(II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter	47:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	2	29	theme	mass	690:693	arg1	spectrometry					695:706	Fourier-transform ion cyclotron resonance mass spectrometry	648:706	Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)	648:718	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	29	theme	mass	690:693	arg1	FT-ICR-MS					709:717	FT-ICR-MS	709:717	FT-ICR-MS	709:717	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	1	30	theme	organic	161:167	arg1	fertilizers					169:179	organic fertilizers	161:179	organic fertilizers	161:179	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	2	31	theme	binding	483:489	arg1	behaviors					491:499	lead (Pb) binding behaviors	473:499	lead (Pb) binding behaviors of DOM	473:506	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	0	32	theme	origins	77:83	arg1	behaviors					60:68	(II) binding behaviors	47:68	(II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter	47:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	7	33	from	polysaccharides	1679:1693	arg1	LMW-DOM					1698:1704	LMW-DOM	1698:1704	LMW-DOM	1698:1704	Pb binding to HMW-DOM derived from SMOF first occurred in the phenolic groups in fulvic-like substances, while polysaccharides in LMW-DOM first participated in the binding of Pb.
37121079	8	34	theme	faster	1873:1878	arg1	response					1880:1887	a faster response	1871:1887	a faster response to Pb	1871:1893	In contrast, irrespective of MWs, polysaccharides and humic-like substances with aromatic (CC) groups in SPOF-DOM displayed a faster response to Pb.
37121079	4	35	theme	ample	1139:1143	arg1	carboxyl					1145:1152	ample carboxyl and hydroxyl groups	1139:1172	carboxyl	1145:1152	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	2	36	dep	weight-dependent	359:374	arg1	behaviors					491:499	lead (Pb) binding behaviors	473:499	lead (Pb) binding behaviors of DOM	473:506	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	36	dep	weight-dependent	359:374	arg1	1 kDa-0.7 µm					406:417	1 kDa-0.7 µm	406:417	1 kDa-0.7 µm	406:417	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	36	dep	weight-dependent	359:374	arg1	compositions					456:467	compositions	456:467	compositions	456:467	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	36	dep	weight-dependent	359:374	arg1	<1 kDa					448:453	<1 kDa	448:453	<1 kDa	448:453	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	37	theme	weight	392:397	arg1	[HMW					399:402	high molecular weight [HMW	377:402	high molecular weight [HMW	377:402	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	37	theme	weight	392:397	arg1	weight-dependent					359:374	the molecular weight-dependent	345:374	the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF)	345:597	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	0	38	theme	organic	88:94	arg1	matter					134:139	organic fertilizers-derived dissolved organic matter	88:139	organic fertilizers-derived dissolved organic matter	88:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	3	39	theme	fluvic-like	878:888	arg1	components					890:899	more fluvic-like components	873:899	more fluvic-like components	873:899	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	2	40	theme	weight	434:439	arg1	[LMW					441:444	low molecular weight [LMW	420:444	low molecular weight [LMW	420:444	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	8	41	with	polysaccharides	1781:1795	arg1	groups					1842:1847	aromatic (CC) groups	1828:1847	aromatic (CC) groups in SPOF-DOM	1828:1859	In contrast, irrespective of MWs, polysaccharides and humic-like substances with aromatic (CC) groups in SPOF-DOM displayed a faster response to Pb.
37121079	2	42	theme	high	377:380	arg1	weight					392:397	high molecular weight	377:397	high molecular weight [HMW	377:402	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	0	43	theme	Molecular	0:8	arg1	compositions					25:36	Molecular size-dependent compositions	0:36	Molecular size-dependent compositions	0:36	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	4	44	from	compounds	1248:1256	arg1	components					1211:1220	HMW-DOM but abundant protein-like components	1177:1220	HMW-DOM but abundant protein-like components	1177:1220	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	2	45	theme	low	420:422	arg1	weight					434:439	low molecular weight	420:439	low molecular weight [LMW	420:444	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	3	46	theme	alicyclic	918:926	arg1	molecules					928:936	carboxylic-rich alicyclic molecules	902:936	carboxylic-rich alicyclic molecules (CRAMs)	902:944	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	46	theme	alicyclic	918:926	arg1	CRAMs					939:943	CRAMs	939:943	CRAMs	939:943	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	10	47	theme	DOM	2169:2171	arg1	sources					2126:2132	sources	2126:2132	sources	2126:2132	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	10	47	theme	DOM	2169:2171	arg1	compositions					2153:2164	size-dependent compositions	2138:2164	size-dependent compositions of DOM	2138:2171	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	7	48	attach	derived	1590:1596	arg2	HMW-DOM					1582:1588	HMW-DOM	1582:1588	HMW-DOM derived from SMOF	1582:1606	Pb binding to HMW-DOM derived from SMOF first occurred in the phenolic groups in fulvic-like substances, while polysaccharides in LMW-DOM first participated in the binding of Pb.
37121079	7	48	attach	derived	1590:1596	arg1	SMOF					1603:1606	SMOF	1603:1606	SMOF	1603:1606	Pb binding to HMW-DOM derived from SMOF first occurred in the phenolic groups in fulvic-like substances, while polysaccharides in LMW-DOM first participated in the binding of Pb.
37121079	6	49	theme	carboxyl	1462:1469	arg1	groups					1471:1476	carboxyl groups	1462:1476	carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM	1462:1520	Moreover, 2D-COS indicated phenol and carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM exhibited superior binding affinities for Pb.
37121079	3	50	theme	lignin	950:955	arg1	compounds					966:974	lignin phenolic compounds	950:974	lignin phenolic compounds	950:974	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	2	51	dep	1 kDa-0.7 µm	406:417	arg1	[LMW					441:444	low molecular weight [LMW	420:444	low molecular weight [LMW	420:444	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	1	52	theme	heavy	309:313	arg1	metals					315:320	heavy metals	309:320	heavy metals	309:320	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	7	53	from	groups	1639:1644	arg1	substances					1661:1670	fulvic-like substances	1649:1670	fulvic-like substances	1649:1670	Pb binding to HMW-DOM derived from SMOF first occurred in the phenolic groups in fulvic-like substances, while polysaccharides in LMW-DOM first participated in the binding of Pb.
37121079	4	54	dep	compounds	1248:1256	arg1	CHOS					1269:1272	CHOS	1269:1272	CHOS	1269:1272	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	4	54	dep	compounds	1248:1256	arg1	CHONS					1259:1263	CHONS	1259:1263	CHONS	1259:1263	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	0	55	link	fertilizers-derived	96:114	arg1	matter					134:139	organic fertilizers-derived dissolved organic matter	88:139	organic fertilizers-derived dissolved organic matter	88:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	5	56	theme	molecular	1403:1411	arg1	diversity					1413:1421	higher molecular diversity	1396:1421	higher molecular diversity	1396:1421	SMOF-DOM exhibited more obvious MW-dependent heterogeneity in molecular components compared to SPOF-DOM with higher molecular diversity.
37121079	6	57	theme	binding	1541:1547	arg1	affinities					1549:1558	superior binding affinities	1532:1558	superior binding affinities for Pb	1532:1565	Moreover, 2D-COS indicated phenol and carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM exhibited superior binding affinities for Pb.
37121079	10	58	theme	size-dependent	2138:2151	arg1	compositions					2153:2164	size-dependent compositions	2138:2164	size-dependent compositions of DOM	2138:2171	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	3	59	theme	microbially-transformed	1007:1029	arg1	compounds					1053:1061	more microbially-transformed heteroatom-containing compounds	1002:1061	more microbially-transformed heteroatom-containing compounds	1002:1061	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	10	60	theme	sources	2126:2132	arg1	effects					2115:2121	the effects	2111:2121	the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments	2111:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	9	61	theme	Pb	2047:2048	arg1	risk					2039:2042	a higher risk	2030:2042	a higher risk of Pb	2030:2048	Furthermore, the polysaccharides which preferentially participated in the binding of Pb to SPOF-DOM and SMOF-derived LMW-DOM may pose a higher risk of Pb in the environment.
37121079	3	62	contain	containing	862:871	arg2	CRAMs					939:943	CRAMs	939:943	CRAMs	939:943	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	62	contain	containing	862:871	arg2	molecules					928:936	carboxylic-rich alicyclic molecules	902:936	carboxylic-rich alicyclic molecules (CRAMs)	902:944	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	62	contain	containing	862:871	arg1	higher					823:828	higher	823:828	higher	823:828	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	62	contain	containing	862:871	arg2	components					890:899	more fluvic-like components	873:899	more fluvic-like components	873:899	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	62	contain	containing	862:871	arg2	compounds					966:974	lignin phenolic compounds	950:974	lignin phenolic compounds	950:974	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	2	63	theme	correlation	740:750	arg1	2D-COS					766:771	2D-COS	766:771	2D-COS	766:771	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	63	theme	correlation	740:750	arg1	spectroscopy					752:763	two-dimensional correlation spectroscopy	724:763	two-dimensional correlation spectroscopy (2D-COS)	724:772	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	10	64	theme	compositions	2153:2164	arg1	effects					2115:2121	the effects	2111:2121	the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments	2111:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	1	65	theme	fertilizers	169:179	arg1	application					146:156	The application	142:156	The application of organic fertilizers	142:179	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	66	theme	large	188:192	arg1	amounts					194:200	large amounts	188:200	large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	188:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	66	theme	large	188:192	arg1	DOM					231:233	DOM	231:233	DOM	231:233	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	66	theme	large	188:192	arg1	metals					315:320	heavy metals	309:320	heavy metals	309:320	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	66	theme	large	188:192	arg1	matter					223:228	dissolved organic matter	205:228	dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	205:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	2	67	theme	peptide-based	558:570	arg1	SPOF					593:596	SPOF	593:596	SPOF	593:596	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	67	theme	peptide-based	558:570	arg1	fertilizers					580:590	shrimp peptide-based organic fertilizers	551:590	shrimp peptide-based organic fertilizers (SPOF)	551:597	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	4	68	theme	more	1110:1113	arg1	compounds					1124:1132	more aromatic compounds	1110:1132	more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components	1110:1220	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	0	69	theme	organic	126:132	arg1	matter					134:139	organic fertilizers-derived dissolved organic matter	88:139	organic fertilizers-derived dissolved organic matter	88:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	2	70	attach	derived	508:514	arg1	sheep					521:525	sheep manure-based	521:538	sheep manure-based (SMOF)	521:545	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	70	attach	derived	508:514	arg1	SMOF					541:544	SMOF	541:544	SMOF	541:544	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	70	attach	derived	508:514	arg1	fertilizers					580:590	shrimp peptide-based organic fertilizers	551:590	shrimp peptide-based organic fertilizers (SPOF)	551:597	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	70	attach	derived	508:514	arg1	SPOF					593:596	SPOF	593:596	SPOF	593:596	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	70	attach	derived	508:514	arg2	compositions					456:467	compositions	456:467	compositions	456:467	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	4	71	from	compounds	1124:1132	arg1	components					1211:1220	HMW-DOM but abundant protein-like components	1177:1220	HMW-DOM but abundant protein-like components	1177:1220	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	2	72	theme	DOM	504:506	arg1	behaviors					491:499	lead (Pb) binding behaviors	473:499	lead (Pb) binding behaviors of DOM	473:506	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	72	theme	DOM	504:506	arg1	compositions					456:467	compositions	456:467	compositions	456:467	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	10	73	theme	associated	2180:2189	arg1	risks					2191:2195	the associated risks	2176:2195	the associated risks of heavy metals in the environments	2176:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	8	74	from	groups	1842:1847	arg1	SPOF-DOM					1852:1859	SPOF-DOM	1852:1859	SPOF-DOM	1852:1859	In contrast, irrespective of MWs, polysaccharides and humic-like substances with aromatic (CC) groups in SPOF-DOM displayed a faster response to Pb.
37121079	1	75	theme	organic	215:221	arg1	DOM					231:233	DOM	231:233	DOM	231:233	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	75	theme	organic	215:221	arg1	matter					223:228	dissolved organic matter	205:228	dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	205:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	4	76	with	compounds	1248:1256	arg1	carboxyl					1145:1152	ample carboxyl and hydroxyl groups	1139:1172	carboxyl	1145:1152	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	4	76	with	compounds	1248:1256	arg1	groups					1167:1172	ample carboxyl and hydroxyl groups	1139:1172	groups	1167:1172	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	4	77	theme	abundant	1189:1196	arg1	components					1211:1220	HMW-DOM but abundant protein-like components	1177:1220	HMW-DOM but abundant protein-like components	1177:1220	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	2	78	theme	manure-based	527:538	arg1	sheep					521:525	sheep manure-based	521:538	sheep manure-based (SMOF)	521:545	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	78	theme	manure-based	527:538	arg1	SMOF					541:544	SMOF	541:544	SMOF	541:544	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	79	theme	ion	666:668	arg1	spectrometry					695:706	Fourier-transform ion cyclotron resonance mass spectrometry	648:706	Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)	648:718	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	79	theme	ion	666:668	arg1	FT-ICR-MS					709:717	FT-ICR-MS	709:717	FT-ICR-MS	709:717	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	3	80	dep	released	800:807	arg1	CHOS					1080:1083	CHOS	1080:1083	CHOS	1080:1083	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	80	dep	released	800:807	arg1	CHONS					1070:1074	CHONS	1070:1074	CHONS	1070:1074	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	80	dep	released	800:807	arg1	CHON					1064:1067	CHON	1064:1067	CHON	1064:1067	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	8	81	theme	aromatic	1828:1835	arg1	groups					1842:1847	aromatic (CC) groups	1828:1847	aromatic (CC) groups in SPOF-DOM	1828:1859	In contrast, irrespective of MWs, polysaccharides and humic-like substances with aromatic (CC) groups in SPOF-DOM displayed a faster response to Pb.
37121079	10	82	theme	heavy	2200:2204	arg1	metals					2206:2211	heavy metals	2200:2211	heavy metals in the environments	2200:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	9	83	theme	higher	2032:2037	arg1	risk					2039:2042	a higher risk	2030:2042	a higher risk of Pb	2030:2048	Furthermore, the polysaccharides which preferentially participated in the binding of Pb to SPOF-DOM and SMOF-derived LMW-DOM may pose a higher risk of Pb in the environment.
37121079	9	84	theme	SPOF-DOM	1987:1994	arg1	LMW-DOM					2013:2019	SPOF-DOM and SMOF-derived LMW-DOM	1987:2019	SPOF-DOM and SMOF-derived LMW-DOM	1987:2019	Furthermore, the polysaccharides which preferentially participated in the binding of Pb to SPOF-DOM and SMOF-derived LMW-DOM may pose a higher risk of Pb in the environment.
37121079	5	85	theme	obvious	1311:1317	arg1	heterogeneity					1332:1344	more obvious MW-dependent heterogeneity	1306:1344	more obvious MW-dependent heterogeneity in molecular components	1306:1368	SMOF-DOM exhibited more obvious MW-dependent heterogeneity in molecular components compared to SPOF-DOM with higher molecular diversity.
37121079	7	86	theme	phenolic	1630:1637	arg1	groups					1639:1644	the phenolic groups	1626:1644	the phenolic groups in fulvic-like substances	1626:1670	Pb binding to HMW-DOM derived from SMOF first occurred in the phenolic groups in fulvic-like substances, while polysaccharides in LMW-DOM first participated in the binding of Pb.
37121079	4	87	theme	HMW-DOM	1177:1183	arg1	components					1211:1220	HMW-DOM but abundant protein-like components	1177:1220	HMW-DOM but abundant protein-like components	1177:1220	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	2	88	theme	chromophoric	605:616	arg1	spectroscopy					634:645	chromophoric and fluorescent spectroscopy	605:645	chromophoric and fluorescent spectroscopy	605:645	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	1	89	dep	matter	223:228	arg1	influencing					270:280	influencing	270:280	influencing the behaviors	270:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	89	dep	matter	223:228	arg1	entering					236:243	entering	236:243	entering the soil environment	236:264	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	3	90	dep	higher	823:828	arg1	aromaticity					830:840	aromaticity	830:840	aromaticity	830:840	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	90	dep	higher	823:828	arg1	hydrophobicity					846:859	hydrophobicity	846:859	hydrophobicity	846:859	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	0	91	theme	fertilizers-derived	96:114	arg1	matter					134:139	organic fertilizers-derived dissolved organic matter	88:139	organic fertilizers-derived dissolved organic matter	88:139	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	10	92	from	environments	2220:2231	arg1	risks					2191:2195	the associated risks	2176:2195	the associated risks of heavy metals in the environments	2176:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	3	93	theme	carboxylic-rich	902:916	arg1	molecules					928:936	carboxylic-rich alicyclic molecules	902:936	carboxylic-rich alicyclic molecules (CRAMs)	902:944	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	3	93	theme	carboxylic-rich	902:916	arg1	CRAMs					939:943	CRAMs	939:943	CRAMs	939:943	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	4	94	theme	heteroatom-containing	1226:1246	arg1	compounds					1248:1256	heteroatom-containing compounds	1226:1256	heteroatom-containing compounds (CHONS and CHOS)	1226:1273	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	2	95	theme	molecular	382:390	arg1	weight					392:397	high molecular weight	377:397	high molecular weight [HMW	377:402	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	0	96	theme	size-dependent	10:23	arg1	compositions					25:36	Molecular size-dependent compositions	0:36	Molecular size-dependent compositions	0:36	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	2	97	theme	molecular	424:432	arg1	weight					434:439	low molecular weight	420:439	low molecular weight [LMW	420:444	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	98	theme	fluorescent	622:632	arg1	spectroscopy					634:645	chromophoric and fluorescent spectroscopy	605:645	chromophoric and fluorescent spectroscopy	605:645	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	0	99	dep	behaviors	60:68	arg1	II					48:49	II	48:49	II	48:49	Molecular size-dependent compositions and lead (II) binding behaviors of two origins of organic fertilizers-derived dissolved organic matter.
37121079	10	100	from	effects	2115:2121	arg1	risks					2191:2195	the associated risks	2176:2195	the associated risks of heavy metals in the environments	2176:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	9	101	theme	SMOF-derived	2000:2011	arg1	LMW-DOM					2013:2019	SPOF-DOM and SMOF-derived LMW-DOM	1987:2019	SPOF-DOM and SMOF-derived LMW-DOM	1987:2019	Furthermore, the polysaccharides which preferentially participated in the binding of Pb to SPOF-DOM and SMOF-derived LMW-DOM may pose a higher risk of Pb in the environment.
37121079	5	102	theme	molecular	1349:1357	arg1	components					1359:1368	molecular components	1349:1368	molecular components	1349:1368	SMOF-DOM exhibited more obvious MW-dependent heterogeneity in molecular components compared to SPOF-DOM with higher molecular diversity.
37121079	10	103	theme	metals	2206:2211	arg1	risks					2191:2195	the associated risks	2176:2195	the associated risks of heavy metals in the environments	2176:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	4	104	with	compounds	1124:1132	arg1	carboxyl					1145:1152	ample carboxyl and hydroxyl groups	1139:1172	carboxyl	1145:1152	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	4	104	with	compounds	1124:1132	arg1	groups					1167:1172	ample carboxyl and hydroxyl groups	1139:1172	groups	1167:1172	Furthermore, there was more aromatic compounds with ample carboxyl and hydroxyl groups in HMW-DOM but abundant protein-like components and heteroatom-containing compounds (CHONS and CHOS) in LMW-DOM.
37121079	3	105	theme	phenolic	957:964	arg1	compounds					966:974	lignin phenolic compounds	950:974	lignin phenolic compounds	950:974	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
37121079	1	106	theme	metals	315:320	arg1	metals					315:320	heavy metals	309:320	heavy metals	309:320	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	106	theme	metals	315:320	arg1	amounts					194:200	large amounts	188:200	large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	188:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	106	theme	metals	315:320	arg1	DOM					231:233	DOM	231:233	DOM	231:233	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	106	theme	metals	315:320	arg1	fates					300:304	fates	300:304	fates of heavy metals	300:320	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	1	106	theme	metals	315:320	arg1	matter					223:228	dissolved organic matter	205:228	dissolved organic matter (DOM) entering the soil environment and influencing the behaviors	205:294	The application of organic fertilizers caused large amounts of dissolved organic matter (DOM) entering the soil environment and influencing the behaviors and fates of heavy metals.
37121079	2	107	theme	resonance	680:688	arg1	spectrometry					695:706	Fourier-transform ion cyclotron resonance mass spectrometry	648:706	Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS)	648:718	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	107	theme	resonance	680:688	arg1	FT-ICR-MS					709:717	FT-ICR-MS	709:717	FT-ICR-MS	709:717	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	5	108	theme	higher	1396:1401	arg1	diversity					1413:1421	higher molecular diversity	1396:1421	higher molecular diversity	1396:1421	SMOF-DOM exhibited more obvious MW-dependent heterogeneity in molecular components compared to SPOF-DOM with higher molecular diversity.
37121079	7	109	theme	Pb	1743:1744	arg1	binding					1732:1738	the binding	1728:1738	the binding of Pb	1728:1744	Pb binding to HMW-DOM derived from SMOF first occurred in the phenolic groups in fulvic-like substances, while polysaccharides in LMW-DOM first participated in the binding of Pb.
37121079	10	110	from	risks	2191:2195	arg1	environments					2220:2231	the environments	2216:2231	the environments	2216:2231	These results were helpful to understand the effects of sources and size-dependent compositions of DOM on the associated risks of heavy metals in the environments.
37121079	2	111	theme	molecular	349:357	arg1	[HMW					399:402	high molecular weight [HMW	377:402	high molecular weight [HMW	377:402	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	2	111	theme	molecular	349:357	arg1	weight-dependent					359:374	the molecular weight-dependent	345:374	the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF)	345:597	Here, we investigated the molecular weight-dependent (high molecular weight [HMW], 1 kDa-0.7 µm; low molecular weight [LMW], <1 kDa) compositions and lead (Pb) binding behaviors of DOM derived from sheep manure-based (SMOF) and shrimp peptide-based organic fertilizers (SPOF) using chromophoric and fluorescent spectroscopy, Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) and two-dimensional correlation spectroscopy (2D-COS).
37121079	6	112	theme	superior	1532:1539	arg1	affinities					1549:1558	superior binding affinities	1532:1558	superior binding affinities for Pb	1532:1565	Moreover, 2D-COS indicated phenol and carboxyl groups in SMOF-DOM and polysaccharides in SPOF-DOM exhibited superior binding affinities for Pb.
37121079	3	113	with	SPOF-DOM	988:995	arg1	compounds					1053:1061	more microbially-transformed heteroatom-containing compounds	1002:1061	more microbially-transformed heteroatom-containing compounds	1002:1061	Results showed that SMOF released more DOM with higher aromaticity and hydrophobicity, containing more fluvic-like components, carboxylic-rich alicyclic molecules (CRAMs) and lignin phenolic compounds compared to SPOF-DOM with more microbially-transformed heteroatom-containing compounds (CHON, CHONS and CHOS).
36941678	12	0	theme	calvarial	2229:2237	arg1	model					2246:2250	the rat calvarial defect model	2221:2250	the rat calvarial defect model	2221:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	14	1	from	insights	2542:2549	arg1	healing					2559:2565	bone healing	2554:2565	bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels	2554:2638	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	3	2	dep	Amount	321:326	arg1	evidence					328:335	evidence	328:335	Amount evidence	321:335	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	5	3	theme	cells	715:719	arg1	roles					673:677	the roles	669:677	the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages	669:860	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	11	4	theme	HUVECs-derived	1683:1696	arg1	exosomes					1698:1705	HUVECs-derived exosomes	1683:1705	HUVECs-derived exosomes	1683:1705	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	13	5	theme	macrophages	2372:2382	arg1	polarization					2356:2367	M2 polarization	2353:2367	M2 polarization of macrophages	2353:2382	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	12	6	theme	decrease	2192:2199	arg1	expression					2207:2216	decrease NLRP3 expression	2192:2216	the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model	2136:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	13	7	with	osteogenesis	2450:2461	arg1	the					2468:2470	the	2468:2470	the	2468:2470	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	14	8	with	healing	2559:2565	arg1	the					2572:2574	the	2572:2574	the	2572:2574	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	12	9	dep	promote	2067:2073	arg1	facilitate					2098:2107	facilitate	2098:2107	facilitate the angiogenesis	2098:2124	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	12	9	dep	promote	2067:2073	arg1	increase					2127:2134	increase	2127:2134	increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model	2127:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	14	10	theme	composite	2620:2628	arg1	hydrogels					2630:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	13	11	theme	DDX3X/NLRP3	2392:2402	arg1	axis					2415:2418	DDX3X/NLRP3 regulatory axis	2392:2418	DDX3X/NLRP3 regulatory axis	2392:2418	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	6	12	theme	enhanced	982:989	arg1	polarization					994:1005	NEAT1 significantly enhanced M2 polarization	962:1005	NEAT1 significantly enhanced M2 polarization	962:1005	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	8	13	theme	nod-like	1367:1374	arg1	protein					1385:1391	nod-like receptor protein 3	1367:1393	nod-like receptor protein 3 (NLRP3)	1367:1401	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	13	theme	nod-like	1367:1374	arg1	NLRP3					1396:1400	NLRP3	1396:1400	NLRP3	1396:1400	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	5	14	theme	human	682:686	arg1	cells					715:719	human umbilical vein endothelial cells	682:719	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	14	theme	human	682:686	arg1	HUVECs					722:727	HUVECs	722:727	HUVECs	722:727	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	14	15	theme	HUVECs-derived	2583:2596	arg1	hydrogels					2630:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	2	16	theme	bone	304:307	arg1	metabolism					309:318	bone metabolism	304:318	bone metabolism	304:318	Macrophages in the microenvironment are instrumental in bone metabolism.
36941678	12	17	theme	macrophages	2169:2179	arg1	expression					2207:2216	decrease NLRP3 expression	2192:2216	the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model	2136:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	12	17	theme	macrophages	2169:2179	arg1	infiltration					2140:2151	the infiltration	2136:2151	the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model	2136:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	6	18	theme	NEAT1	962:966	arg1	polarization					994:1005	NEAT1 significantly enhanced M2 polarization	962:1005	NEAT1 significantly enhanced M2 polarization	962:1005	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	7	19	from	macrophages	1094:1104	arg1	medium					1082:1087	the conditioned medium	1066:1087	the conditioned medium from macrophages induced by the exosomes	1066:1128	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	5	20	theme	vein	698:701	arg1	cells					715:719	human umbilical vein endothelial cells	682:719	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	20	theme	vein	698:701	arg1	HUVECs					722:727	HUVECs	722:727	HUVECs	722:727	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	8	21	contain	carrying	1272:1279	arg1	Exos					1267:1270	Exos	1267:1270	Exos carrying NEAT1	1267:1285	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	21	contain	carrying	1272:1279	arg2	NEAT1					1281:1285	NEAT1	1281:1285	NEAT1	1281:1285	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	22	theme	helicase	1336:1343	arg1	expression					1313:1322	expression	1313:1322	expression of dead-box helicase 3X-linked (DDX3X)	1313:1361	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	22	theme	helicase	1336:1343	arg1	protein					1385:1391	nod-like receptor protein 3	1367:1393	nod-like receptor protein 3 (NLRP3)	1367:1401	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	22	theme	helicase	1336:1343	arg1	NLRP3					1396:1400	NLRP3	1396:1400	NLRP3	1396:1400	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	11	23	dep	cytokines	1784:1792	arg1	cytokines					1784:1792	decreased pro-inflammatory cytokines	1757:1792	decreased pro-inflammatory cytokines (IL-1β and IL-6)	1757:1809	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	11	23	dep	cytokines	1784:1792	arg1	IL-6					1805:1808	IL-6	1805:1808	IL-6	1805:1808	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	11	23	dep	cytokines	1784:1792	arg1	IL-1β					1795:1799	IL-1β	1795:1799	IL-1β	1795:1799	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	5	24	theme	enrichment	764:773	arg1	NEAT1					798:802	NEAT1	798:802	NEAT1	798:802	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	24	theme	enrichment	764:773	arg1	transcript					784:793	nuclear enrichment enriched transcript 1	756:795	nuclear enrichment enriched transcript 1 (NEAT1)	756:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	12	25	from	expression	2207:2216	arg1	model					2246:2250	the rat calvarial defect model	2221:2250	the rat calvarial defect model	2221:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	13	26	theme	M2	2353:2354	arg1	polarization					2356:2367	M2 polarization	2353:2367	M2 polarization of macrophages	2353:2382	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	12	27	theme	M2	2156:2157	arg1	macrophages					2169:2179	M2 polarized macrophages	2156:2179	M2 polarized macrophages	2156:2179	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	5	28	theme	-derived	729:736	arg1	exosomes					738:745	-derived exosomes	729:745	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	29	theme	present	629:635	arg1	study					642:646	the present time study	625:646	the present time study	625:646	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	1	30	theme	BACKGROUND	128:137	arg1	regeneration					144:155	BACKGROUND Bone regeneration	128:155	BACKGROUND Bone regeneration	128:155	BACKGROUND Bone regeneration is a complex procedure that involves an interaction between osteogenesis and inflammation.
36941678	1	30	theme	BACKGROUND	128:137	arg1	procedure					170:178	a complex procedure	160:178	a complex procedure that involves an interaction between osteogenesis and inflammation	160:245	BACKGROUND Bone regeneration is a complex procedure that involves an interaction between osteogenesis and inflammation.
36941678	2	31	from	metabolism	309:318	arg1	instrumental					288:299	instrumental	288:299	instrumental	288:299	Macrophages in the microenvironment are instrumental in bone metabolism.
36941678	13	32	theme	HUVECs-derived	2265:2278	arg1	exosomes					2280:2287	HUVECs-derived exosomes	2265:2287	HUVECs-derived exosomes	2265:2287	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	5	33	from	roles	673:677	arg1	osteogenesis					812:823	the osteogenesis	808:823	the osteogenesis mediated by M2 polarized macrophages	808:860	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	3	34	theme	exosomes	356:363	arg1	lncRNA					378:383	exosomes transmitting lncRNA	356:383	exosomes transmitting lncRNA	356:383	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	11	35	theme	model	1659:1663	arg1	results					1634:1640	The results	1630:1640	The results of air pouch rat model	1630:1663	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	13	36	dep	the	2468:2470	arg1	aid					2472:2474	aid	2472:2474	aid	2472:2474	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	14	37	dep	the	2572:2574	arg1	aid					2576:2578	aid	2576:2578	aid	2576:2578	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	11	38	theme	pouch	1649:1653	arg1	model					1659:1663	air pouch rat model	1645:1663	air pouch rat model	1645:1663	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	9	39	theme	NLRP3	1417:1421	arg1	protein					1423:1429	NLRP3 protein	1417:1429	NLRP3 protein	1417:1429	The level of NLRP3 protein increased significantly after RAW264.7 cells transfected with DDX3X overexpression plasmid.
36941678	2	40	from	Macrophages	248:258	arg1	microenvironment					267:282	the microenvironment	263:282	the microenvironment	263:282	Macrophages in the microenvironment are instrumental in bone metabolism.
36941678	3	41	theme	crucial	388:394	arg1	nanocarriers					396:407	crucial nanocarriers	388:407	crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis	388:488	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	10	42	theme	Exos	1624:1627	arg1	effect					1614:1619	the aforementioned effect	1595:1619	the aforementioned effect of Exos	1595:1627	Additionally, the knockdown of NEAT1 in exosomes partially counteracted the aforementioned effect of Exos.
36941678	7	43	link	marrow-derived	1206:1219	arg1	BMSCs					1245:1249	BMSCs	1245:1249	BMSCs	1245:1249	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	7	43	link	marrow-derived	1206:1219	arg1	cells					1238:1242	bone marrow-derived mesenchymal stem cells	1201:1242	bone marrow-derived mesenchymal stem cells (BMSCs)	1201:1250	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	0	44	theme	DDX3X/NLRP3	110:120	arg1	axis					122:125	DDX3X/NLRP3 axis	110:125	DDX3X/NLRP3 axis	110:125	Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.
36941678	12	45	theme	bone	2079:2082	arg1	regeneration					2084:2095	the bone regeneration	2075:2095	the bone regeneration	2075:2095	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	3	46	theme	various	438:444	arg1	procedures					453:462	various biotic procedures	438:462	various biotic procedures	438:462	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	4	47	theme	relationship	544:555	arg1	mechanisms					515:524	the underlying mechanisms	500:524	the underlying mechanisms of the regulatory relationship between the exosomes and macrophages	500:592	However, the underlying mechanisms of the regulatory relationship between the exosomes and macrophages are awaiting clarification.
36941678	5	48	theme	enriched	775:782	arg1	NEAT1					798:802	NEAT1	798:802	NEAT1	798:802	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	48	theme	enriched	775:782	arg1	transcript					784:793	nuclear enrichment enriched transcript 1	756:795	nuclear enrichment enriched transcript 1 (NEAT1)	756:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	0	49	theme	Exosomal	0:7	arg1	NEAT1					13:17	Exosomal Lnc NEAT1	0:17	Exosomal Lnc NEAT1 from endothelial cells	0:40	Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.
36941678	5	50	theme	underlying	880:889	arg1	mechanisms					891:900	the underlying mechanisms	876:900	the underlying mechanisms	876:900	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	6	51	dep	RESULTS	903:909	arg1	attenuated					1011:1020	attenuated	1011:1020	attenuated LPS-induced inflammation in vitro	1011:1054	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	6	51	dep	RESULTS	903:909	arg1	demonstrated					914:925	demonstrated	914:925	demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization	914:1005	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	11	52	theme	LPS-induced	1866:1876	arg1	inflammation					1878:1889	LPS-induced inflammation	1866:1889	LPS-induced inflammation	1866:1889	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	0	53	theme	endothelial	24:34	arg1	cells					36:40	endothelial cells	24:40	endothelial cells	24:40	Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.
36941678	5	54	theme	M2	837:838	arg1	macrophages					850:860	M2 polarized macrophages	837:860	M2 polarized macrophages	837:860	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	6	55	theme	HUVECs-derived	927:940	arg1	exosomes					942:949	HUVECs-derived exosomes	927:949	HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization	927:1005	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	13	56	dep	CONCLUSIONS	2253:2263	arg1	enable					2289:2294	enable	2289:2294	enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo	2289:2514	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	11	57	theme	decreased	1757:1765	arg1	cytokines					1784:1792	decreased pro-inflammatory cytokines	1757:1792	decreased pro-inflammatory cytokines (IL-1β and IL-6)	1757:1809	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	11	57	theme	decreased	1757:1765	arg1	IL-6					1805:1808	IL-6	1805:1808	IL-6	1805:1808	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	11	57	theme	decreased	1757:1765	arg1	IL-1β					1795:1799	IL-1β	1795:1799	IL-1β	1795:1799	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	6	58	link	HUVECs-derived	927:940	arg1	exosomes					942:949	HUVECs-derived exosomes	927:949	HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization	927:1005	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	9	59	theme	overexpression	1499:1512	arg1	plasmid					1514:1520	DDX3X overexpression plasmid	1493:1520	DDX3X overexpression plasmid	1493:1520	The level of NLRP3 protein increased significantly after RAW264.7 cells transfected with DDX3X overexpression plasmid.
36941678	12	60	theme	network	2037:2043	arg1	hydrogels					2051:2059	polymer network (IPN) hydrogels	2029:2059	polymer network (IPN) hydrogels	2029:2059	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	7	61	theme	cells	1238:1242	arg1	migration					1157:1165	migration	1157:1165	migration	1157:1165	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	7	61	theme	cells	1238:1242	arg1	differentiation					1182:1196	osteogenic differentiation	1171:1196	osteogenic differentiation	1171:1196	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	12	62	theme	polymer	2029:2035	arg1	hydrogels					2051:2059	polymer network (IPN) hydrogels	2029:2059	polymer network (IPN) hydrogels	2029:2059	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	13	63	theme	regulatory	2404:2413	arg1	axis					2415:2418	DDX3X/NLRP3 regulatory axis	2392:2418	DDX3X/NLRP3 regulatory axis	2392:2418	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	12	64	theme	IPN	2046:2048	arg1	hydrogels					2051:2059	polymer network (IPN) hydrogels	2029:2059	polymer network (IPN) hydrogels	2029:2059	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	12	65	from	infiltration	2140:2151	arg1	model					2246:2250	the rat calvarial defect model	2221:2250	the rat calvarial defect model	2221:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	12	66	theme	alginate/gelatin	1974:1989	arg1	methacrylate					1991:2002	alginate/gelatin methacrylate	1974:2002	alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels	1974:2059	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	12	66	theme	alginate/gelatin	1974:1989	arg1	GelMA					2005:2009	GelMA	2005:2009	GelMA	2005:2009	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	13	67	theme	IPN	2494:2496	arg1	hydrogels					2498:2506	alginate/GelMA IPN hydrogels	2479:2506	alginate/GelMA IPN hydrogels	2479:2506	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	5	68	theme	polarized	840:848	arg1	macrophages					850:860	M2 polarized macrophages	837:860	M2 polarized macrophages	837:860	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	6	69	theme	LPS-induced	1022:1032	arg1	inflammation					1034:1045	LPS-induced inflammation	1022:1045	LPS-induced inflammation	1022:1045	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	10	70	from	knockdown	1541:1549	arg1	exosomes					1563:1570	exosomes	1563:1570	exosomes	1563:1570	Additionally, the knockdown of NEAT1 in exosomes partially counteracted the aforementioned effect of Exos.
36941678	12	71	link	HUVECs-derived	1934:1947	arg1	exosomes					1949:1956	the HUVECs-derived exosomes	1930:1956	the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels	1930:2059	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	5	72	theme	endothelial	703:713	arg1	cells					715:719	human umbilical vein endothelial cells	682:719	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	72	theme	endothelial	703:713	arg1	HUVECs					722:727	HUVECs	722:727	HUVECs	722:727	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	14	73	theme	tissue	2691:2696	arg1	engineering					2698:2708	bone tissue engineering	2686:2708	bone tissue engineering	2686:2708	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	12	74	theme	rat	2225:2227	arg1	model					2246:2250	the rat calvarial defect model	2221:2250	the rat calvarial defect model	2221:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	7	75	theme	marrow-derived	1206:1219	arg1	BMSCs					1245:1249	BMSCs	1245:1249	BMSCs	1245:1249	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	7	75	theme	marrow-derived	1206:1219	arg1	cells					1238:1242	bone marrow-derived mesenchymal stem cells	1201:1242	bone marrow-derived mesenchymal stem cells (BMSCs)	1201:1250	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	12	76	theme	HUVECs-derived	1934:1947	arg1	exosomes					1949:1956	the HUVECs-derived exosomes	1930:1956	the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels	1930:2059	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	11	77	link	HUVECs-derived	1683:1696	arg1	exosomes					1698:1705	HUVECs-derived exosomes	1683:1705	HUVECs-derived exosomes	1683:1705	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	6	78	theme	M2	991:992	arg1	polarization					994:1005	NEAT1 significantly enhanced M2 polarization	962:1005	NEAT1 significantly enhanced M2 polarization	962:1005	RESULTS We demonstrated HUVECs-derived exosomes expressing NEAT1 significantly enhanced M2 polarization and attenuated LPS-induced inflammation in vitro.
36941678	7	79	dep	migration	1157:1165	arg1	the					1153:1155	the	1153:1155	the	1153:1155	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	7	80	theme	stem	1233:1236	arg1	BMSCs					1245:1249	BMSCs	1245:1249	BMSCs	1245:1249	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	7	80	theme	stem	1233:1236	arg1	cells					1238:1242	bone marrow-derived mesenchymal stem cells	1201:1242	bone marrow-derived mesenchymal stem cells (BMSCs)	1201:1250	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	14	81	theme	exosomes-encapsulated	2598:2618	arg1	hydrogels					2630:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	12	82	theme	NLRP3	2201:2205	arg1	expression					2207:2216	decrease NLRP3 expression	2192:2216	the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model	2136:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	14	83	theme	engineering	2698:2708	arg1	use					2679:2681	the use	2675:2681	the use of bone tissue engineering	2675:2708	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	4	84	dep	exosomes	569:576	arg1	the					565:567	the	565:567	the	565:567	However, the underlying mechanisms of the regulatory relationship between the exosomes and macrophages are awaiting clarification.
36941678	5	85	theme	umbilical	688:696	arg1	cells					715:719	human umbilical vein endothelial cells	682:719	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	85	theme	umbilical	688:696	arg1	HUVECs					722:727	HUVECs	722:727	HUVECs	722:727	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	10	86	theme	NEAT1	1554:1558	arg1	knockdown					1541:1549	the knockdown	1537:1549	the knockdown of NEAT1 in exosomes	1537:1570	Additionally, the knockdown of NEAT1 in exosomes partially counteracted the aforementioned effect of Exos.
36941678	12	87	theme	polarized	2159:2167	arg1	macrophages					2169:2179	M2 polarized macrophages	2156:2179	M2 polarized macrophages	2156:2179	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	7	88	theme	mesenchymal	1221:1231	arg1	BMSCs					1245:1249	BMSCs	1245:1249	BMSCs	1245:1249	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	7	88	theme	mesenchymal	1221:1231	arg1	cells					1238:1242	bone marrow-derived mesenchymal stem cells	1201:1242	bone marrow-derived mesenchymal stem cells (BMSCs)	1201:1250	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	8	89	theme	receptor	1376:1383	arg1	protein					1385:1391	nod-like receptor protein 3	1367:1393	nod-like receptor protein 3 (NLRP3)	1367:1401	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	89	theme	receptor	1376:1383	arg1	NLRP3					1396:1400	NLRP3	1396:1400	NLRP3	1396:1400	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	5	90	theme	nuclear	756:762	arg1	NEAT1					798:802	NEAT1	798:802	NEAT1	798:802	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	90	theme	nuclear	756:762	arg1	transcript					784:793	nuclear enrichment enriched transcript 1	756:795	nuclear enrichment enriched transcript 1 (NEAT1)	756:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	11	91	theme	anti-inflammatory	1717:1733	arg1	IL-10					1746:1750	IL-10	1746:1750	IL-10	1746:1750	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	11	91	theme	anti-inflammatory	1717:1733	arg1	cytokines					1735:1743	anti-inflammatory cytokines	1717:1743	anti-inflammatory cytokines (IL-10)	1717:1751	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	8	92	theme	dead-box	1327:1334	arg1	helicase					1336:1343	dead-box helicase 3X-linked	1327:1353	dead-box helicase 3X-linked (DDX3X)	1327:1361	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	92	theme	dead-box	1327:1334	arg1	DDX3X					1356:1360	DDX3X	1356:1360	DDX3X	1356:1360	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	4	93	theme	underlying	504:513	arg1	mechanisms					515:524	the underlying mechanisms	500:524	the underlying mechanisms of the regulatory relationship between the exosomes and macrophages	500:592	However, the underlying mechanisms of the regulatory relationship between the exosomes and macrophages are awaiting clarification.
36941678	7	94	theme	osteogenic	1171:1180	arg1	differentiation					1182:1196	osteogenic differentiation	1171:1196	osteogenic differentiation	1171:1196	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	8	95	theme	3X-linked	1345:1353	arg1	helicase					1336:1343	dead-box helicase 3X-linked	1327:1353	dead-box helicase 3X-linked (DDX3X)	1327:1361	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	95	theme	3X-linked	1345:1353	arg1	DDX3X					1356:1360	DDX3X	1356:1360	DDX3X	1356:1360	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	5	96	theme	time	637:640	arg1	study					642:646	the present time study	625:646	the present time study	625:646	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	1	97	theme	Bone	139:142	arg1	regeneration					144:155	BACKGROUND Bone regeneration	128:155	BACKGROUND Bone regeneration	128:155	BACKGROUND Bone regeneration is a complex procedure that involves an interaction between osteogenesis and inflammation.
36941678	1	97	theme	Bone	139:142	arg1	procedure					170:178	a complex procedure	160:178	a complex procedure that involves an interaction between osteogenesis and inflammation	160:245	BACKGROUND Bone regeneration is a complex procedure that involves an interaction between osteogenesis and inflammation.
36941678	14	98	theme	bone	2554:2557	arg1	healing					2559:2565	bone healing	2554:2565	bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels	2554:2638	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	13	99	theme	alginate/GelMA	2479:2492	arg1	hydrogels					2498:2506	alginate/GelMA IPN hydrogels	2479:2506	alginate/GelMA IPN hydrogels	2479:2506	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	12	100	theme	defect	2239:2244	arg1	model					2246:2250	the rat calvarial defect model	2221:2250	the rat calvarial defect model	2221:2250	Afterwards, we further confirmed that the HUVECs-derived exosomes encapsulated in alginate/gelatin methacrylate (GelMA) interpenetrating polymer network (IPN) hydrogels could promote the bone regeneration, facilitate the angiogenesis, increase the infiltration of M2 polarized macrophages as well as decrease NLRP3 expression in the rat calvarial defect model.
36941678	11	101	theme	rat	1655:1657	arg1	model					1659:1663	air pouch rat model	1645:1663	air pouch rat model	1645:1663	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	0	102	theme	macrophage	82:91	arg1	polarization					93:104	macrophage polarization	82:104	macrophage polarization	82:104	Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.
36941678	1	103	theme	complex	162:168	arg1	regeneration					144:155	BACKGROUND Bone regeneration	128:155	BACKGROUND Bone regeneration	128:155	BACKGROUND Bone regeneration is a complex procedure that involves an interaction between osteogenesis and inflammation.
36941678	1	103	theme	complex	162:168	arg1	procedure					170:178	a complex procedure	160:178	a complex procedure that involves an interaction between osteogenesis and inflammation	160:245	BACKGROUND Bone regeneration is a complex procedure that involves an interaction between osteogenesis and inflammation.
36941678	3	104	theme	transmitting	365:376	arg1	lncRNA					378:383	exosomes transmitting lncRNA	356:383	exosomes transmitting lncRNA	356:383	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	11	105	theme	air	1645:1647	arg1	model					1659:1663	air pouch rat model	1645:1663	air pouch rat model	1645:1663	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	7	106	theme	conditioned	1070:1080	arg1	medium					1082:1087	the conditioned medium	1066:1087	the conditioned medium from macrophages induced by the exosomes	1066:1128	Besides, the conditioned medium from macrophages induced by the exosomes indirectly facilitated the migration and osteogenic differentiation of bone marrow-derived mesenchymal stem cells (BMSCs).
36941678	3	107	theme	cellular	413:420	arg1	interactions					422:433	cellular interactions	413:433	cellular interactions in various biotic procedures, especially, osteogenesis	413:488	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	4	108	theme	regulatory	533:542	arg1	relationship					544:555	the regulatory relationship	529:555	the regulatory relationship between the exosomes and macrophages	529:592	However, the underlying mechanisms of the regulatory relationship between the exosomes and macrophages are awaiting clarification.
36941678	9	109	theme	protein	1423:1429	arg1	level					1408:1412	The level	1404:1412	The level of NLRP3 protein	1404:1429	The level of NLRP3 protein increased significantly after RAW264.7 cells transfected with DDX3X overexpression plasmid.
36941678	0	110	from	cells	36:40	arg1	NEAT1					13:17	Exosomal Lnc NEAT1	0:17	Exosomal Lnc NEAT1 from endothelial cells	0:40	Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.
36941678	0	111	theme	Lnc	9:11	arg1	NEAT1					13:17	Exosomal Lnc NEAT1	0:17	Exosomal Lnc NEAT1 from endothelial cells	0:40	Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.
36941678	14	112	link	HUVECs-derived	2583:2596	arg1	hydrogels					2630:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	HUVECs-derived exosomes-encapsulated composite hydrogels	2583:2638	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	3	113	theme	biotic	446:451	arg1	procedures					453:462	various biotic procedures	438:462	various biotic procedures	438:462	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	10	114	theme	aforementioned	1599:1612	arg1	effect					1614:1619	the aforementioned effect	1595:1619	the aforementioned effect of Exos	1595:1627	Additionally, the knockdown of NEAT1 in exosomes partially counteracted the aforementioned effect of Exos.
36941678	9	115	theme	RAW264.7	1461:1468	arg1	cells					1470:1474	RAW264.7 cells	1461:1474	RAW264.7 cells transfected with DDX3X overexpression plasmid	1461:1520	The level of NLRP3 protein increased significantly after RAW264.7 cells transfected with DDX3X overexpression plasmid.
36941678	8	116	link	3X-linked	1345:1353	arg1	helicase					1336:1343	dead-box helicase 3X-linked	1327:1353	dead-box helicase 3X-linked (DDX3X)	1327:1361	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	8	116	link	3X-linked	1345:1353	arg1	DDX3X					1356:1360	DDX3X	1356:1360	DDX3X	1356:1360	Mechanically, Exos carrying NEAT1 decreased remarkably both expression of dead-box helicase 3X-linked (DDX3X) and nod-like receptor protein 3 (NLRP3).
36941678	5	117	dep	cells	715:719	arg1	exosomes					738:745	-derived exosomes	729:745	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	13	118	link	HUVECs-derived	2265:2278	arg1	exosomes					2280:2287	HUVECs-derived exosomes	2265:2287	HUVECs-derived exosomes	2265:2287	CONCLUSIONS HUVECs-derived exosomes enable transmitting NEAT1 to alleviate inflammation by inducing M2 polarization of macrophages through DDX3X/NLRP3 regulatory axis, which finally contributes to osteogenesis with the aid of alginate/GelMA IPN hydrogels in vivo.
36941678	2	119	from	instrumental	288:299	arg1	metabolism					309:318	bone metabolism	304:318	bone metabolism	304:318	Macrophages in the microenvironment are instrumental in bone metabolism.
36941678	5	120	contain	carrying	747:754	arg1	exosomes					738:745	-derived exosomes	729:745	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	120	contain	carrying	747:754	arg2	transcript					784:793	nuclear enrichment enriched transcript 1	756:795	nuclear enrichment enriched transcript 1 (NEAT1)	756:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	5	120	contain	carrying	747:754	arg2	NEAT1					798:802	NEAT1	798:802	NEAT1	798:802	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	14	121	theme	bone	2686:2689	arg1	engineering					2698:2708	bone tissue engineering	2686:2708	bone tissue engineering	2686:2708	Thus, our study provides insights in bone healing with the aid of HUVECs-derived exosomes-encapsulated composite hydrogels, which exhibited potential towards the use of bone tissue engineering in the foreseeable future.
36941678	0	122	theme	bone	50:53	arg1	regeneration					55:66	bone regeneration	50:66	bone regeneration	50:66	Exosomal Lnc NEAT1 from endothelial cells promote bone regeneration by regulating macrophage polarization via DDX3X/NLRP3 axis.
36941678	9	123	theme	DDX3X	1493:1497	arg1	plasmid					1514:1520	DDX3X overexpression plasmid	1493:1520	DDX3X overexpression plasmid	1493:1520	The level of NLRP3 protein increased significantly after RAW264.7 cells transfected with DDX3X overexpression plasmid.
36941678	11	124	theme	inflammation	1878:1889	arg1	amelioration					1850:1861	amelioration	1850:1861	amelioration of LPS-induced inflammation	1850:1889	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	3	125	from	osteogenesis	477:488	arg1	procedures					453:462	various biotic procedures	438:462	various biotic procedures	438:462	Amount evidence have revealed that exosomes transmitting lncRNA is crucial nanocarriers for cellular interactions in various biotic procedures, especially, osteogenesis.
36941678	5	126	link	-derived	729:736	arg1	exosomes					738:745	-derived exosomes	729:745	human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1)	682:803	In the present time study, we aimed to explore the roles of human umbilical vein endothelial cells (HUVECs)-derived exosomes carrying nuclear enrichment enriched transcript 1 (NEAT1) in the osteogenesis mediated by M2 polarized macrophages and elucidate the underlying mechanisms.
36941678	11	127	theme	pro-inflammatory	1767:1782	arg1	cytokines					1784:1792	decreased pro-inflammatory cytokines	1757:1792	decreased pro-inflammatory cytokines (IL-1β and IL-6)	1757:1809	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	11	127	theme	pro-inflammatory	1767:1782	arg1	IL-6					1805:1808	IL-6	1805:1808	IL-6	1805:1808	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36941678	11	127	theme	pro-inflammatory	1767:1782	arg1	IL-1β					1795:1799	IL-1β	1795:1799	IL-1β	1795:1799	The results of air pouch rat model demonstrated that HUVECs-derived exosomes increased anti-inflammatory cytokines (IL-10) and decreased pro-inflammatory cytokines (IL-1β and IL-6) significantly in vivo, contributing to amelioration of LPS-induced inflammation.
36929341	8	0	theme	diagnostic	1473:1482	arg1	component					1497:1505	the predominant menaquinone and diagnostic diamino acid component	1441:1505	component	1497:1505	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	10	1	theme	cryptoxanthini	1846:1859	arg1	sp					1861:1862	the name Ornithinimicrobium cryptoxanthini sp	1818:1862	the name Ornithinimicrobium cryptoxanthini sp	1818:1862	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	8	2	theme	acid	1492:1495	arg1	component					1497:1505	the predominant menaquinone and diagnostic diamino acid component	1441:1505	component	1497:1505	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	7	3	dep	strains	1311:1317	arg1	strains					1311:1317	strains JY.X269 and JY.X270T	1311:1338	strains JY.X269 and JY.X270T	1311:1338	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	3	dep	strains	1311:1317	arg1	JY.X270T					1331:1338	JY.X270T	1331:1338	JY.X270T	1331:1338	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	3	dep	strains	1311:1317	arg1	JY.X269					1319:1325	JY.X269	1319:1325	JY.X269	1319:1325	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	4	theme	cellular	1277:1284	arg1	acids					1292:1296	the major cellular fatty acids	1267:1296	the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T	1267:1338	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	4	theme	cellular	1277:1284	arg1	%					1305:1305	> 10.0%	1299:1305	> 10.0%	1299:1305	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	4	theme	cellular	1277:1284	arg1	iso-C15:0					1345:1353	iso-C15:0	1345:1353	iso-C15:0	1345:1353	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	11	5	theme	1.19147T = JCM	1912:1925	arg1	34882T					1927:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
36929341	2	6	theme	novel	154:158	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	6	theme	novel	154:158	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	6	theme	novel	154:158	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	6	7	theme	other	1086:1090	arg1	species					1111:1117	other Ornithinimicrobium species	1086:1117	other Ornithinimicrobium species	1086:1117	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	11	8	theme	strain	1888:1893	arg1	34882T					1927:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
36929341	6	9	theme	identity	1012:1019	arg1	values					1027:1032	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values	948:1032	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species	948:1117	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	11	10	dep	proposed	1873:1880	arg1	34882T					1927:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
36929341	2	11	theme	river	326:330	arg1	sediments					313:321	the near-surface sediments	296:321	the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019	296:401	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	8	12	theme	cell	1518:1521	arg1	wall					1523:1526	the cell wall teichoic acids	1514:1541	the cell wall teichoic acids	1514:1541	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	2	13	theme	rod-shaped	234:243	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	13	theme	rod-shaped	234:243	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	13	theme	rod-shaped	234:243	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	6	14	theme	average	993:999	arg1	ANI					1022:1024	ANI	1022:1024	ANI	1022:1024	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	6	14	theme	average	993:999	arg1	identity					1012:1019	average nucleotide identity	993:1019	average nucleotide identity (ANI)	993:1025	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	2	15	dep	bacteria	245:252	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	15	dep	bacteria	245:252	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	15	dep	bacteria	245:252	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	6	16	theme	%	1169:1169	arg1	ranges					1135:1140	the ranges	1131:1140	the ranges of 19.0-23.9% and 70.8-80.4%, respectively	1131:1183	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	3	17	theme	%	493:493	arg1	NaCl					501:504	0-6.0% (w/v) NaCl	488:504	0-6.0% (w/v) NaCl	488:504	Both strains were shown to grow at 15-35 °C and pH 7.0-10.0, and in the presence of 0-6.0% (w/v) NaCl.
36929341	8	18	theme	teichoic	1528:1535	arg1	acids					1537:1541	teichoic acids	1528:1541	the cell wall teichoic acids	1514:1541	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	1	19	attach	Isolated	106:113	arg1	Sediments					139:147	the Tongtian River Sediments	120:147	the Tongtian River Sediments	120:147	nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.
36929341	1	19	attach	Isolated	106:113	arg2	β-cryptoxanthin					90:104	a Novel Actinomycete Producing β-cryptoxanthin	59:104	a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments	59:147	nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.
36929341	3	20	theme	NaCl	501:504	arg1	presence					476:483	the presence	472:483	the presence of 0-6.0% (w/v) NaCl	472:504	Both strains were shown to grow at 15-35 °C and pH 7.0-10.0, and in the presence of 0-6.0% (w/v) NaCl.
36929341	1	21	theme	River	133:137	arg1	Sediments					139:147	the Tongtian River Sediments	120:147	the Tongtian River Sediments	120:147	nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.
36929341	6	22	dep	isolates	1050:1057	arg1	JY.X269					1060:1066	JY.X269	1060:1066	JY.X269	1060:1066	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	6	22	dep	isolates	1050:1057	arg1	isolates					1050:1057	two isolates	1046:1057	two isolates (JY.X269 and JY.X270T)	1046:1080	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	6	22	dep	isolates	1050:1057	arg1	JY.X270T					1072:1079	JY.X270T	1072:1079	JY.X270T	1072:1079	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	5	23	theme	16S	805:807	arg1	gene					814:817	the 16S rRNA gene and 537 core gene sequences	801:845	gene	814:817	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	4	24	theme	%	642:642	arg1	30183 T					624:630	Ornithinimicrobium cavernae CFH 30183 T	592:630	Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity)	592:677	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	24	theme	%	642:642	arg1	similarity					667:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	25	theme	Ornithinimicrobium	592:609	arg1	similarity					667:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	25	theme	Ornithinimicrobium	592:609	arg1	30183 T					624:630	Ornithinimicrobium cavernae CFH 30183 T	592:630	Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity)	592:677	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	6	26	theme	recommended	1211:1221	arg1	%					1227:1227	70.0%	1223:1227	70.0%	1223:1227	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	0	27	theme	Ornithinimicrobium	15:32	arg1	cryptoxanthini					34:47	Ornithinimicrobium cryptoxanthini	15:47	Ornithinimicrobium cryptoxanthini	15:47	Description of Ornithinimicrobium cryptoxanthini sp.
36929341	3	28	dep	%	493:493	arg1	w/v					496:498	w/v	496:498	w/v	496:498	Both strains were shown to grow at 15-35 °C and pH 7.0-10.0, and in the presence of 0-6.0% (w/v) NaCl.
36929341	7	29	theme	summed	1371:1376	arg1	feature					1378:1384	summed feature 9	1371:1386	summed feature 9	1371:1386	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	4	30	theme	CFH	620:622	arg1	similarity					667:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	30	theme	CFH	620:622	arg1	30183 T					624:630	Ornithinimicrobium cavernae CFH 30183 T	592:630	Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity)	592:677	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	8	31	dep	wall	1523:1526	arg1	acids					1537:1541	teichoic acids	1528:1541	the cell wall teichoic acids	1514:1541	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	10	32	theme	Ornithinimicrobium	1788:1805	arg1	strains					1728:1734	the two strains	1720:1734	the two strains	1720:1734	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	10	32	theme	Ornithinimicrobium	1788:1805	arg1	species					1767:1773	a novel species	1759:1773	a novel species	1759:1773	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	5	33	theme	gene	832:835	arg1	sequences					837:845	the 16S rRNA gene and 537 core gene sequences	801:845	sequences	837:845	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	4	34	theme	O.	680:681	arg1	H23M54T					692:698	O. ciconiae H23M54T	680:698	O. ciconiae H23M54T (98.5-98.6%)	680:711	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	34	theme	O.	680:681	arg1	%					710:710	98.5-98.6%	701:710	98.5-98.6%	701:710	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	1	35	theme	Producing	80:88	arg1	β-cryptoxanthin					90:104	a Novel Actinomycete Producing β-cryptoxanthin	59:104	a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments	59:147	nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.
36929341	8	36	contain	contained	1405:1413	arg1	JY.X270T					1396:1403	Strain JY.X270T	1389:1403	Strain JY.X270T	1389:1403	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	8	36	contain	contained	1405:1413	arg2	menaquinone					1457:1467	the predominant menaquinone and diagnostic diamino acid component	1441:1505	menaquinone	1457:1467	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	8	36	contain	contained	1405:1413	arg2	H4					1420:1421	H4	1420:1421	H4	1420:1421	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	8	36	contain	contained	1405:1413	arg2	MK-8					1415:1418	MK-8	1415:1418	MK-8(H4)	1415:1422	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	8	36	contain	contained	1405:1413	arg2	ornithine					1428:1436	ornithine	1428:1436	ornithine	1428:1436	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	4	37	theme	rRNA	648:651	arg1	30183 T					624:630	Ornithinimicrobium cavernae CFH 30183 T	592:630	Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity)	592:677	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	37	theme	rRNA	648:651	arg1	similarity					667:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	9	38	theme	strain	1592:1597	arg1	JY.X270T					1599:1606	strain JY.X270T	1592:1606	strain JY.X270T	1592:1606	β-cryptoxanthin (C40H56O) can be extracted from strain JY.X270T, and its content is 6.3 µg/ml.
36929341	4	39	theme	sequence	658:665	arg1	30183 T					624:630	Ornithinimicrobium cavernae CFH 30183 T	592:630	Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity)	592:677	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	39	theme	sequence	658:665	arg1	similarity					667:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	6	40	theme	cutoff	1240:1245	arg1	point					1247:1251	cutoff point	1240:1251	cutoff point	1240:1251	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	6	41	dep	%	1227:1227	arg1	point					1247:1251	cutoff point	1240:1251	cutoff point	1240:1251	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	5	42	theme	537	823:825	arg1	sequences					837:845	the 16S rRNA gene and 537 core gene sequences	801:845	sequences	837:845	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	2	43	theme	non-motile	190:199	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	43	theme	non-motile	190:199	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	43	theme	non-motile	190:199	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	44	theme	aerobic	181:187	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	44	theme	aerobic	181:187	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	44	theme	aerobic	181:187	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	4	45	theme	O.	717:718	arg1	01-Gi-040T					727:736	O. murale 01-Gi-040T	717:736	O. murale 01-Gi-040T (98.3-98.5%)	717:749	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	45	theme	O.	717:718	arg1	%					748:748	98.3-98.5%	739:748	98.3-98.5%	739:748	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	10	46	theme	phenotypic	1699:1708	arg1	analyses					1710:1717	the phylogenetic, chemotaxonomic, and phenotypic analyses	1661:1717	the phylogenetic, chemotaxonomic, and phenotypic analyses	1661:1717	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	0	47	dep	sp	49:50	arg1	Description					0:10	Description	0:10	Description of Ornithinimicrobium cryptoxanthini	0:47	Description of Ornithinimicrobium cryptoxanthini sp.
36929341	10	48	theme	Ornithinimicrobium	1827:1844	arg1	sp					1861:1862	the name Ornithinimicrobium cryptoxanthini sp	1818:1862	the name Ornithinimicrobium cryptoxanthini sp	1818:1862	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	2	49	dep	13″N	372:375	arg1	37″E					384:387	37″E	384:387	37″E	384:387	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	6	50	theme	hybridization	968:980	arg1	values					1027:1032	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values	948:1032	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species	948:1117	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	4	51	theme	16S	511:513	arg1	rRNA					515:518	The 16S rRNA	507:518	The 16S rRNA gene sequence analysis	507:541	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	6	52	theme	digital	952:958	arg1	dDDH					983:986	dDDH	983:986	dDDH	983:986	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	6	52	theme	digital	952:958	arg1	hybridization					968:980	digital DNA-DNA hybridization	952:980	digital DNA-DNA hybridization (dDDH)	952:987	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	4	53	theme	gene	520:523	arg1	analysis					534:541	The 16S rRNA gene sequence analysis	507:541	The 16S rRNA gene sequence analysis	507:541	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	10	54	theme	chemotaxonomic	1679:1692	arg1	analyses					1710:1717	the phylogenetic, chemotaxonomic, and phenotypic analyses	1661:1717	the phylogenetic, chemotaxonomic, and phenotypic analyses	1661:1717	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	8	55	theme	predominant	1445:1455	arg1	menaquinone					1457:1467	the predominant menaquinone and diagnostic diamino acid component	1441:1505	menaquinone	1457:1467	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	8	55	theme	predominant	1445:1455	arg1	ornithine					1428:1436	ornithine	1428:1436	ornithine	1428:1436	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	8	55	theme	predominant	1445:1455	arg1	MK-8					1415:1418	MK-8	1415:1418	MK-8(H4)	1415:1422	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	7	56	theme	fatty	1286:1290	arg1	acids					1292:1296	the major cellular fatty acids	1267:1296	the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T	1267:1338	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	56	theme	fatty	1286:1290	arg1	%					1305:1305	> 10.0%	1299:1305	> 10.0%	1299:1305	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	56	theme	fatty	1286:1290	arg1	iso-C15:0					1345:1353	iso-C15:0	1345:1353	iso-C15:0	1345:1353	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	11	57	theme	type	1883:1886	arg1	34882T					1927:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
36929341	10	58	theme	phylogenetic	1665:1676	arg1	analyses					1710:1717	the phylogenetic, chemotaxonomic, and phenotypic analyses	1661:1717	the phylogenetic, chemotaxonomic, and phenotypic analyses	1661:1717	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	2	59	theme	near-surface	300:311	arg1	sediments					313:321	the near-surface sediments	296:321	the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019	296:401	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	7	60	theme	major	1271:1275	arg1	acids					1292:1296	the major cellular fatty acids	1267:1296	the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T	1267:1338	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	60	theme	major	1271:1275	arg1	%					1305:1305	> 10.0%	1299:1305	> 10.0%	1299:1305	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	60	theme	major	1271:1275	arg1	iso-C15:0					1345:1353	iso-C15:0	1345:1353	iso-C15:0	1345:1353	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	1	61	dep	β-cryptoxanthin	90:104	arg1	nov.					53:56	nov.	53:56	nov.	53:56	nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.
36929341	2	62	theme	Gram-stain-positive	160:178	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	62	theme	Gram-stain-positive	160:178	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	62	theme	Gram-stain-positive	160:178	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	6	63	theme	Ornithinimicrobium	1092:1109	arg1	species					1111:1117	other Ornithinimicrobium species	1086:1117	other Ornithinimicrobium species	1086:1117	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	8	64	theme	diamino	1484:1490	arg1	component					1497:1505	the predominant menaquinone and diagnostic diamino acid component	1441:1505	component	1497:1505	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	4	65	theme	murale	720:725	arg1	01-Gi-040T					727:736	O. murale 01-Gi-040T	717:736	O. murale 01-Gi-040T (98.3-98.5%)	717:749	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	65	theme	murale	720:725	arg1	%					748:748	98.3-98.5%	739:748	98.3-98.5%	739:748	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	2	66	from	China	359:363	arg1	sediments					313:321	the near-surface sediments	296:321	the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019	296:401	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	11	67	theme	JY.X270T = CGMCC	1895:1910	arg1	34882T					1927:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	type strain JY.X270T = CGMCC 1.19147T = JCM 34882T	1883:1932	nov. is proposed (type strain JY.X270T = CGMCC 1.19147T = JCM 34882T).
36929341	6	68	theme	nucleotide	1001:1010	arg1	ANI					1022:1024	ANI	1022:1024	ANI	1022:1024	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	6	68	theme	nucleotide	1001:1010	arg1	identity					1012:1019	average nucleotide identity	993:1019	average nucleotide identity (ANI)	993:1025	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	3	69	theme	0-6.0	488:492	arg1	%					493:493	%	493:493	%	493:493	Both strains were shown to grow at 15-35 °C and pH 7.0-10.0, and in the presence of 0-6.0% (w/v) NaCl.
36929341	5	70	theme	above	927:931	arg1	species					939:945	the above three species	923:945	the above three species	923:945	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	6	71	theme	%	1154:1154	arg1	ranges					1135:1140	the ranges	1131:1140	the ranges of 19.0-23.9% and 70.8-80.4%, respectively	1131:1183	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	10	72	from	analyses	1710:1717	arg1	results					1648:1654	results	1648:1654	results from the phylogenetic, chemotaxonomic, and phenotypic analyses	1648:1717	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	2	73	attach	isolated	282:289	arg2	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	73	attach	isolated	282:289	arg2	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	73	attach	isolated	282:289	arg1	sediments					313:321	the near-surface sediments	296:321	the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019	296:401	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	73	attach	isolated	282:289	arg2	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	5	74	theme	distinct	901:908	arg1	cluster					910:916	a distinct cluster	899:916	a distinct cluster	899:916	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	4	75	theme	98.6-98.8	633:641	arg1	%					642:642	%	642:642	%	642:642	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	5	76	theme	phylogenetic	756:767	arg1	trees					786:790	The phylogenetic and phylogenomic trees	752:790	The phylogenetic and phylogenomic trees	752:790	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	8	77	theme	Strain	1389:1394	arg1	JY.X270T					1396:1403	Strain JY.X270T	1389:1403	Strain JY.X270T	1389:1403	Strain JY.X270T contained MK-8(H4) and ornithine as the predominant menaquinone and diagnostic diamino acid component within the cell wall teichoic acids.
36929341	10	78	theme	novel	1761:1765	arg1	strains					1728:1734	the two strains	1720:1734	the two strains	1720:1734	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	10	78	theme	novel	1761:1765	arg1	species					1767:1773	a novel species	1759:1773	a novel species	1759:1773	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	6	79	theme	respective	1200:1209	arg1	%					1227:1227	70.0%	1223:1227	70.0%	1223:1227	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	5	80	theme	phylogenomic	773:784	arg1	trees					786:790	The phylogenetic and phylogenomic trees	752:790	The phylogenetic and phylogenomic trees	752:790	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	2	81	from	sediments	313:321	arg1	China					359:363	China	359:363	China	359:363	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	81	from	sediments	313:321	arg1	P.					353:354	P.	353:354	P.	353:354	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	5	82	theme	core	827:830	arg1	sequences					837:845	the 16S rRNA gene and 537 core gene sequences	801:845	sequences	837:845	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	2	83	from	river	326:330	arg1	China					359:363	China	359:363	China	359:363	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	83	from	river	326:330	arg1	P.					353:354	P.	353:354	P.	353:354	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	1	84	theme	Novel	61:65	arg1	β-cryptoxanthin					90:104	a Novel Actinomycete Producing β-cryptoxanthin	59:104	a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments	59:147	nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.
36929341	10	85	theme	genus	1782:1786	arg1	Ornithinimicrobium					1788:1805	the genus Ornithinimicrobium	1778:1805	the genus Ornithinimicrobium	1778:1805	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	1	86	theme	Actinomycete	67:78	arg1	β-cryptoxanthin					90:104	a Novel Actinomycete Producing β-cryptoxanthin	59:104	a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments	59:147	nov., a Novel Actinomycete Producing β-cryptoxanthin Isolated from the Tongtian River Sediments.
36929341	5	87	theme	rRNA	809:812	arg1	gene					814:817	the 16S rRNA gene and 537 core gene sequences	801:845	gene	814:817	The phylogenetic and phylogenomic trees based on the 16S rRNA gene and 537 core gene sequences, respectively, revealed that the two strains formed a distinct cluster with the above three species.
36929341	4	88	theme	16S	644:646	arg1	30183 T					624:630	Ornithinimicrobium cavernae CFH 30183 T	592:630	Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity)	592:677	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	88	theme	16S	644:646	arg1	similarity					667:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	0	89	theme	cryptoxanthini	34:47	arg1	Description					0:10	Description	0:10	Description of Ornithinimicrobium cryptoxanthini	0:47	Description of Ornithinimicrobium cryptoxanthini sp.
36929341	4	90	dep	Ornithinimicrobium	592:609	arg1	cavernae					611:618	cavernae	611:618	cavernae	611:618	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	91	theme	gene	653:656	arg1	30183 T					624:630	Ornithinimicrobium cavernae CFH 30183 T	592:630	Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity)	592:677	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	91	theme	gene	653:656	arg1	similarity					667:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	98.6-98.8% 16S rRNA gene sequence similarity	633:676	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	2	92	theme	irregular	224:232	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	92	theme	irregular	224:232	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	92	theme	irregular	224:232	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	7	93	theme	strains	1311:1317	arg1	acids					1292:1296	the major cellular fatty acids	1267:1296	the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T	1267:1338	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	93	theme	strains	1311:1317	arg1	%					1305:1305	> 10.0%	1299:1305	> 10.0%	1299:1305	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	7	93	theme	strains	1311:1317	arg1	iso-C15:0					1345:1353	iso-C15:0	1345:1353	iso-C15:0	1345:1353	Furthermore, the major cellular fatty acids (> 10.0%) of strains JY.X269 and JY.X270T were iso-C15:0, iso-C16:0, and summed feature 9.
36929341	10	94	theme	name	1822:1825	arg1	sp					1861:1862	the name Ornithinimicrobium cryptoxanthini sp	1818:1862	the name Ornithinimicrobium cryptoxanthini sp	1818:1862	Based on results from the phylogenetic, chemotaxonomic, and phenotypic analyses, the two strains could be classified as a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cryptoxanthini sp.
36929341	4	95	theme	rRNA	515:518	arg1	analysis					534:541	The 16S rRNA gene sequence analysis	507:541	The 16S rRNA gene sequence analysis	507:541	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	2	96	theme	yellow-pigmented	206:221	arg1	JY.X270T					267:274	JY.X270T	267:274	JY.X270T	267:274	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	96	theme	yellow-pigmented	206:221	arg1	JY.X269					255:261	JY.X269	255:261	JY.X269	255:261	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	2	96	theme	yellow-pigmented	206:221	arg1	bacteria					245:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria	150:252	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T)	150:275	Two novel Gram-stain-positive, aerobic, non-motile, and yellow-pigmented, irregular rod-shaped bacteria (JY.X269 and JY.X270T) were isolated from the near-surface sediments of river in Qinghai Province, P. R. China (32°37'13″N, 96°05'37″E) in July 2019.
36929341	4	97	theme	ciconiae	683:690	arg1	H23M54T					692:698	O. ciconiae H23M54T	680:698	O. ciconiae H23M54T (98.5-98.6%)	680:711	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	4	97	theme	ciconiae	683:690	arg1	%					710:710	98.5-98.6%	701:710	98.5-98.6%	701:710	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
36929341	6	98	theme	DNA-DNA	960:966	arg1	dDDH					983:986	dDDH	983:986	dDDH	983:986	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	6	98	theme	DNA-DNA	960:966	arg1	hybridization					968:980	digital DNA-DNA hybridization	952:980	digital DNA-DNA hybridization (dDDH)	952:987	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between our two isolates (JY.X269 and JY.X270T) and other Ornithinimicrobium species were within the ranges of 19.0-23.9% and 70.8-80.4%, respectively, all below the respective recommended 70.0% and 95-96% cutoff point.
36929341	4	99	theme	sequence	525:532	arg1	analysis					534:541	The 16S rRNA gene sequence analysis	507:541	The 16S rRNA gene sequence analysis	507:541	The 16S rRNA gene sequence analysis showed that the isolates were closely related to Ornithinimicrobium cavernae CFH 30183 T (98.6-98.8% 16S rRNA gene sequence similarity), O. ciconiae H23M54T (98.5-98.6%) and O. murale 01-Gi-040T (98.3-98.5%).
35838582	7	0	theme	sphingolipids	1192:1204	arg1	levels					1157:1162	the levels	1153:1162	the levels of glycerophospholipids and sphingolipids in BAT	1153:1211	L-carnitine significantly increased the levels of TG and diglyceride (DG) and decreased the levels of glycerophospholipids and sphingolipids in BAT.
35838582	2	1	theme	BAT	340:342	arg1	adipogenesis					344:355	BAT adipogenesis	340:355	BAT adipogenesis	340:355	In the previous study, we found that L-carnitine was enriched in BAT and promoted BAT adipogenesis and thermogenesis in goat brown adipocytes.
35838582	0	2	theme	goats	92:96	arg1	thermogenesis					67:79	brown adipose tissue thermogenesis	46:79	brown adipose tissue thermogenesis of newborn goats	46:96	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	7	3	theme	TG	1115:1116	arg1	levels					1105:1110	the levels	1101:1110	the levels of TG and diglyceride (DG)	1101:1137	L-carnitine significantly increased the levels of TG and diglyceride (DG) and decreased the levels of glycerophospholipids and sphingolipids in BAT.
35838582	1	4	theme	important	152:160	arg1	component					162:170	an important component	149:170	an important component of energy expenditure and necessary to maintain body temperature for newborn mammals	149:255	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	4	theme	important	152:160	arg1	tissue					133:138	Brown adipose tissue	119:138	Brown adipose tissue (BAT)	119:144	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	4	5	from	thermogenesis	681:693	arg1	goats					706:710	newborn goats	698:710	newborn goats	698:710	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	4	6	theme	rectal	582:587	arg1	temperature					589:599	the rectal temperature	578:599	the rectal temperature	578:599	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	5	7	theme	maternal	723:730	arg1	supplementation					744:758	maternal L-carnitine supplementation	723:758	maternal L-carnitine supplementation	723:758	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	9	8	theme	cold	1536:1539	arg1	exposure					1541:1548	cold exposure	1536:1548	cold exposure	1536:1548	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	9	9	theme	new	1461:1463	arg1	evidence					1465:1472	new evidence	1461:1472	new evidence for newborn goats	1461:1490	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	4	10	theme	content	658:664	arg1	temperature					589:599	the rectal temperature	578:599	the rectal temperature	578:599	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	4	10	theme	content	658:664	arg1	expression					617:626	the expression	613:626	the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats	613:710	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	0	11	theme	cold	104:107	arg1	exposure					109:116	cold exposure	104:116	cold exposure	104:116	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	5	12	theme	fatty	819:823	arg1	NEFA					832:835	NEFA	832:835	NEFA	832:835	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	12	theme	fatty	819:823	arg1	acids					825:829	non-esterified fatty acids	804:829	non-esterified fatty acids (NEFA)	804:836	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	13	theme	newborn	925:931	arg1	goats					933:937	newborn goats	925:937	newborn goats	925:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	4	14	theme	newborn	698:704	arg1	goats					706:710	newborn goats	698:710	newborn goats	698:710	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	9	15	theme	L-carnitine	1360:1370	arg1	supplementation					1372:1386	maternal L-carnitine supplementation	1351:1386	maternal L-carnitine supplementation	1351:1386	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	5	16	theme	lactate	843:849	arg1	levels					774:779	the levels	770:779	the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma	770:859	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	16	theme	lactate	843:849	arg1	content					877:883	the content	873:883	the content of lipid droplet and glycogen in BAT of newborn goats	873:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	9	17	theme	body	1504:1507	arg1	temperature					1509:1519	body temperature	1504:1519	body temperature	1504:1519	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	5	18	theme	non-esterified	804:817	arg1	NEFA					832:835	NEFA	832:835	NEFA	832:835	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	18	theme	non-esterified	804:817	arg1	acids					825:829	non-esterified fatty acids	804:829	non-esterified fatty acids (NEFA)	804:836	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	19	theme	goats	933:937	arg1	BAT					918:920	BAT	918:920	BAT of newborn goats	918:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	2	20	theme	brown	383:387	arg1	adipocytes					389:398	goat brown adipocytes	378:398	goat brown adipocytes	378:398	In the previous study, we found that L-carnitine was enriched in BAT and promoted BAT adipogenesis and thermogenesis in goat brown adipocytes.
35838582	6	21	theme	Lipidomic	940:948	arg1	analysis					950:957	Lipidomic analysis	940:957	Lipidomic analysis	940:957	Lipidomic analysis showed that maternal L-carnitine supplementation remodeled the lipid composition of BAT in newborn goats.
35838582	2	22	theme	previous	265:272	arg1	study					274:278	the previous study	261:278	the previous study	261:278	In the previous study, we found that L-carnitine was enriched in BAT and promoted BAT adipogenesis and thermogenesis in goat brown adipocytes.
35838582	2	23	theme	goat	378:381	arg1	adipocytes					389:398	goat brown adipocytes	378:398	goat brown adipocytes	378:398	In the previous study, we found that L-carnitine was enriched in BAT and promoted BAT adipogenesis and thermogenesis in goat brown adipocytes.
35838582	0	24	theme	L-carnitine	9:19	arg1	supplementation					21:35	Maternal L-carnitine supplementation	0:35	Maternal L-carnitine supplementation	0:35	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	5	25	theme	triglycerides	784:796	arg1	levels					774:779	the levels	770:779	the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma	770:859	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	25	theme	triglycerides	784:796	arg1	content					877:883	the content	873:883	the content of lipid droplet and glycogen in BAT of newborn goats	873:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	3	26	from	expenditure	479:489	arg1	lambs					494:498	lambs	494:498	lambs	494:498	However, whether dietary L-carnitine regulates BAT heat production and energy expenditure in lambs remains unclear.
35838582	1	27	theme	energy	175:180	arg1	expenditure					182:192	energy expenditure	175:192	energy expenditure	175:192	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	0	28	theme	Maternal	0:7	arg1	supplementation					21:35	Maternal L-carnitine supplementation	0:35	Maternal L-carnitine supplementation	0:35	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	5	29	from	content	877:883	arg1	BAT					918:920	BAT	918:920	BAT of newborn goats	918:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	29	from	content	877:883	arg1	plasma					854:859	plasma	854:859	plasma	854:859	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	30	theme	glycogen	906:913	arg1	levels					774:779	the levels	770:779	the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma	770:859	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	30	theme	glycogen	906:913	arg1	content					877:883	the content	873:883	the content of lipid droplet and glycogen in BAT of newborn goats	873:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	1	31	theme	expenditure	182:192	arg1	component					162:170	an important component	149:170	an important component of energy expenditure and necessary to maintain body temperature for newborn mammals	149:255	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	31	theme	expenditure	182:192	arg1	tissue					133:138	Brown adipose tissue	119:138	Brown adipose tissue (BAT)	119:144	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	5	32	theme	acids	825:829	arg1	levels					774:779	the levels	770:779	the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma	770:859	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	32	theme	acids	825:829	arg1	content					877:883	the content	873:883	the content of lipid droplet and glycogen in BAT of newborn goats	873:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	4	33	theme	L-carnitine	541:551	arg1	supplementation					553:567	maternal L-carnitine supplementation	532:567	maternal L-carnitine supplementation	532:567	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	6	34	from	composition	1028:1038	arg1	goats					1058:1062	newborn goats	1050:1062	newborn goats	1050:1062	Lipidomic analysis showed that maternal L-carnitine supplementation remodeled the lipid composition of BAT in newborn goats.
35838582	6	35	theme	lipid	1022:1026	arg1	composition					1028:1038	the lipid composition	1018:1038	the lipid composition of BAT in newborn goats	1018:1062	Lipidomic analysis showed that maternal L-carnitine supplementation remodeled the lipid composition of BAT in newborn goats.
35838582	6	36	theme	newborn	1050:1056	arg1	goats					1058:1062	newborn goats	1050:1062	newborn goats	1050:1062	Lipidomic analysis showed that maternal L-carnitine supplementation remodeled the lipid composition of BAT in newborn goats.
35838582	4	37	theme	UCP1	631:634	arg1	temperature					589:599	the rectal temperature	578:599	the rectal temperature	578:599	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	4	37	theme	UCP1	631:634	arg1	expression					617:626	the expression	613:626	the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats	613:710	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	7	38	from	levels	1157:1162	arg1	BAT					1209:1211	BAT	1209:1211	BAT	1209:1211	L-carnitine significantly increased the levels of TG and diglyceride (DG) and decreased the levels of glycerophospholipids and sphingolipids in BAT.
35838582	1	39	theme	Brown	119:123	arg1	component					162:170	an important component	149:170	an important component of energy expenditure and necessary to maintain body temperature for newborn mammals	149:255	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	39	theme	Brown	119:123	arg1	BAT					141:143	BAT	141:143	BAT	141:143	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	39	theme	Brown	119:123	arg1	tissue					133:138	Brown adipose tissue	119:138	Brown adipose tissue (BAT)	119:144	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	40	theme	necessary	198:206	arg1	component					162:170	an important component	149:170	an important component of energy expenditure and necessary to maintain body temperature for newborn mammals	149:255	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	40	theme	necessary	198:206	arg1	tissue					133:138	Brown adipose tissue	119:138	Brown adipose tissue (BAT)	119:144	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	0	41	theme	adipose	52:58	arg1	thermogenesis					67:79	brown adipose tissue thermogenesis	46:79	brown adipose tissue thermogenesis of newborn goats	46:96	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	4	42	theme	mitochondrial	640:652	arg1	content					658:664	mitochondrial DNA content	640:664	mitochondrial DNA content	640:664	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	3	43	theme	dietary	418:424	arg1	L-carnitine					426:436	dietary L-carnitine	418:436	dietary L-carnitine	418:436	However, whether dietary L-carnitine regulates BAT heat production and energy expenditure in lambs remains unclear.
35838582	3	44	theme	BAT	448:450	arg1	production					457:466	BAT heat production	448:466	BAT heat production	448:466	However, whether dietary L-carnitine regulates BAT heat production and energy expenditure in lambs remains unclear.
35838582	0	45	theme	brown	46:50	arg1	thermogenesis					67:79	brown adipose tissue thermogenesis	46:79	brown adipose tissue thermogenesis of newborn goats	46:96	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	9	46	theme	maternal	1351:1358	arg1	supplementation					1372:1386	maternal L-carnitine supplementation	1351:1386	maternal L-carnitine supplementation	1351:1386	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	8	47	theme	brown	1293:1297	arg1	adipocytes					1299:1308	brown adipocytes	1293:1308	brown adipocytes	1293:1308	Further studies showed that L-carnitine promoted TG and glycogen deposition in brown adipocytes through AMPKα.
35838582	5	48	theme	L-carnitine	732:742	arg1	supplementation					744:758	maternal L-carnitine supplementation	723:758	maternal L-carnitine supplementation	723:758	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	3	49	theme	heat	452:455	arg1	production					457:466	BAT heat production	448:466	BAT heat production	448:466	However, whether dietary L-carnitine regulates BAT heat production and energy expenditure in lambs remains unclear.
35838582	7	50	theme	glycerophospholipids	1167:1186	arg1	levels					1157:1162	the levels	1153:1162	the levels of glycerophospholipids and sphingolipids in BAT	1153:1211	L-carnitine significantly increased the levels of TG and diglyceride (DG) and decreased the levels of glycerophospholipids and sphingolipids in BAT.
35838582	1	51	theme	adipose	125:131	arg1	component					162:170	an important component	149:170	an important component of energy expenditure and necessary to maintain body temperature for newborn mammals	149:255	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	51	theme	adipose	125:131	arg1	BAT					141:143	BAT	141:143	BAT	141:143	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	1	51	theme	adipose	125:131	arg1	tissue					133:138	Brown adipose tissue	119:138	Brown adipose tissue (BAT)	119:144	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	0	52	theme	tissue	60:65	arg1	thermogenesis					67:79	brown adipose tissue thermogenesis	46:79	brown adipose tissue thermogenesis of newborn goats	46:96	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	4	53	theme	maternal	532:539	arg1	supplementation					553:567	maternal L-carnitine supplementation	532:567	maternal L-carnitine supplementation	532:567	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	9	54	from	thermogenesis	1417:1429	arg1	goats					1442:1446	newborn goats	1434:1446	newborn goats	1434:1446	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	3	55	from	production	457:466	arg1	lambs					494:498	lambs	494:498	lambs	494:498	However, whether dietary L-carnitine regulates BAT heat production and energy expenditure in lambs remains unclear.
35838582	8	56	theme	glycogen	1270:1277	arg1	deposition					1279:1288	TG and glycogen deposition	1263:1288	deposition	1279:1288	Further studies showed that L-carnitine promoted TG and glycogen deposition in brown adipocytes through AMPKα.
35838582	7	57	theme	diglyceride	1122:1132	arg1	levels					1105:1110	the levels	1101:1110	the levels of TG and diglyceride (DG)	1101:1137	L-carnitine significantly increased the levels of TG and diglyceride (DG) and decreased the levels of glycerophospholipids and sphingolipids in BAT.
35838582	3	58	theme	energy	472:477	arg1	expenditure					479:489	energy expenditure	472:489	energy expenditure	472:489	However, whether dietary L-carnitine regulates BAT heat production and energy expenditure in lambs remains unclear.
35838582	9	59	theme	BAT	1397:1399	arg1	development					1401:1411	BAT development	1397:1411	BAT development	1397:1411	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	1	60	theme	body	220:223	arg1	temperature					225:235	body temperature	220:235	body temperature for newborn mammals	220:255	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35838582	9	61	from	development	1401:1411	arg1	goats					1442:1446	newborn goats	1434:1446	newborn goats	1434:1446	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	9	62	theme	newborn	1434:1440	arg1	goats					1442:1446	newborn goats	1434:1446	newborn goats	1434:1446	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	6	63	theme	BAT	1043:1045	arg1	composition					1028:1038	the lipid composition	1018:1038	the lipid composition of BAT in newborn goats	1018:1062	Lipidomic analysis showed that maternal L-carnitine supplementation remodeled the lipid composition of BAT in newborn goats.
35838582	5	64	theme	droplet	894:900	arg1	levels					774:779	the levels	770:779	the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma	770:859	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	64	theme	droplet	894:900	arg1	content					877:883	the content	873:883	the content of lipid droplet and glycogen in BAT of newborn goats	873:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	65	theme	lipid	888:892	arg1	droplet					894:900	lipid droplet	888:900	lipid droplet	888:900	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	0	66	theme	newborn	84:90	arg1	goats					92:96	newborn goats	84:96	newborn goats	84:96	Maternal L-carnitine supplementation promotes brown adipose tissue thermogenesis of newborn goats after cold exposure.
35838582	5	67	from	levels	774:779	arg1	BAT					918:920	BAT	918:920	BAT of newborn goats	918:937	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	5	67	from	levels	774:779	arg1	plasma					854:859	plasma	854:859	plasma	854:859	Moreover, maternal L-carnitine supplementation increased the levels of triglycerides (TG), non-esterified fatty acids (NEFA), and lactate in plasma, as well as the content of lipid droplet and glycogen in BAT of newborn goats.
35838582	8	68	theme	TG	1263:1264	arg1	deposition					1279:1288	TG and glycogen deposition	1263:1288	deposition	1279:1288	Further studies showed that L-carnitine promoted TG and glycogen deposition in brown adipocytes through AMPKα.
35838582	9	69	theme	newborn	1478:1484	arg1	goats					1486:1490	newborn goats	1478:1490	newborn goats	1478:1490	Our results indicate that maternal L-carnitine supplementation promotes BAT development and thermogenesis in newborn goats and provides new evidence for newborn goats to maintain body temperature in response to cold exposure.
35838582	4	70	theme	DNA	654:656	arg1	content					658:664	mitochondrial DNA content	640:664	mitochondrial DNA content	640:664	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	6	71	theme	L-carnitine	980:990	arg1	supplementation					992:1006	maternal L-carnitine supplementation	971:1006	maternal L-carnitine supplementation	971:1006	Lipidomic analysis showed that maternal L-carnitine supplementation remodeled the lipid composition of BAT in newborn goats.
35838582	8	72	theme	Further	1214:1220	arg1	studies					1222:1228	Further studies	1214:1228	Further studies	1214:1228	Further studies showed that L-carnitine promoted TG and glycogen deposition in brown adipocytes through AMPKα.
35838582	6	73	theme	maternal	971:978	arg1	supplementation					992:1006	maternal L-carnitine supplementation	971:1006	maternal L-carnitine supplementation	971:1006	Lipidomic analysis showed that maternal L-carnitine supplementation remodeled the lipid composition of BAT in newborn goats.
35838582	4	74	theme	BAT	677:679	arg1	thermogenesis					681:693	BAT thermogenesis	677:693	BAT thermogenesis in newborn goats	677:710	In this study, maternal L-carnitine supplementation elevated the rectal temperature, as well as the expression of UCP1 and mitochondrial DNA content to promote BAT thermogenesis in newborn goats.
35838582	1	75	theme	newborn	241:247	arg1	mammals					249:255	newborn mammals	241:255	newborn mammals	241:255	Brown adipose tissue (BAT) is an important component of energy expenditure and necessary to maintain body temperature for newborn mammals.
35889417	2	0	theme	asthmatic	585:593	arg1	lungs					595:599	allergic and asthmatic lungs	572:599	allergic and asthmatic lungs	572:599	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	10	1	from	conditions	1884:1893	arg1	mice					1898:1901	mice	1898:1901	mice	1898:1901	These results indicate that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3, under physiological conditions in mice.
35889417	2	2	theme	allergic	572:579	arg1	lungs					595:599	allergic and asthmatic lungs	572:599	allergic and asthmatic lungs	572:599	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	10	3	theme	sulfated	1741:1748	arg1	glycans					1750:1756	sulfated glycans	1741:1756	sulfated glycans	1741:1756	These results indicate that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3, under physiological conditions in mice.
35889417	9	4	theme	GlcNAc6ST1/3/4	1610:1623	arg1	triple-knock-out					1625:1640	GlcNAc6ST1/3/4 triple-knock-out	1610:1640	GlcNAc6ST1/3/4 triple-knock-out	1610:1640	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	1	5	theme	6-sulfo	168:174	arg1	sLeX					176:179	6-sulfo sLeX	168:179	6-sulfo sLeX	168:179	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	5	theme	6-sulfo	168:174	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	7	6	located	detected	1161:1168	arg2	expression					1113:1122	the expression	1109:1122	the expression of the CL40 epitope	1109:1142	Here, we show that the expression of the CL40 epitope was specifically detected in the mesothelin-positive mesothelium of the pulmonary pleura.
35889417	7	6	located	detected	1161:1168	arg1	mesothelium					1197:1207	the mesothelin-positive mesothelium	1173:1207	the mesothelin-positive mesothelium of the pulmonary pleura	1173:1231	Here, we show that the expression of the CL40 epitope was specifically detected in the mesothelin-positive mesothelium of the pulmonary pleura.
35889417	6	7	theme	GlcNAc-6-O-sulfotransferases	977:1004	arg1	contribution					961:972	The contribution	957:972	The contribution of GlcNAc-6-O-sulfotransferases (GlcNAc6STs) to the synthesis of the CL40 epitope in the lungs	957:1067	The contribution of GlcNAc-6-O-sulfotransferases (GlcNAc6STs) to the synthesis of the CL40 epitope in the lungs was also elucidated.
35889417	2	8	theme	aggregate	526:534	arg1	vasculature					536:546	the lymphoid aggregate vasculature	513:546	the lymphoid aggregate vasculature	513:546	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	9	9	theme	in	1527:1528	arg1	expression					1535:1544	in vivo expression	1527:1544	in vivo expression of the CL40 epitope in the lung mesothelium	1527:1588	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	0	10	theme	Mouse	117:121	arg1	Mesothelium					131:141	the Mouse Pleural Mesothelium	113:141	the Mouse Pleural Mesothelium	113:141	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	1	11	theme	organs	363:368	arg1	HEVs					335:338	HEVs	335:338	HEVs	335:338	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	11	theme	organs	363:368	arg1	venules					326:332	the high endothelial venules	305:332	the high endothelial venules (HEVs) of secondary lymphoid organs	305:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	12	theme	sialyl	201:206	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	12	theme	sialyl	201:206	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	12	theme	sialyl	201:206	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	10	13	theme	pleural	1778:1784	arg1	mesothelium					1786:1796	the pleural mesothelium	1774:1796	the pleural mesothelium	1774:1796	These results indicate that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3, under physiological conditions in mice.
35889417	8	14	theme	glycans	1429:1435	arg1	synthesis					1402:1410	the synthesis	1398:1410	the synthesis of CL40-positive glycans in the lung mesothelium	1398:1459	Moreover, GlcNAc6ST2 (encoded by Chst4) and GlcNAc6ST3 (encoded by Chst5), but not GlcNAc6ST1 (encoded by Chst2) or GlcNAc6ST4 (encoded by Chst7), are required for the synthesis of CL40-positive glycans in the lung mesothelium.
35889417	1	15	theme	endothelial	314:324	arg1	HEVs					335:338	HEVs	335:338	HEVs	335:338	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	15	theme	endothelial	314:324	arg1	venules					326:332	the high endothelial venules	305:332	the high endothelial venules (HEVs) of secondary lymphoid organs	305:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	5	16	theme	normal	900:905	arg1	lungs					913:917	normal mouse lungs	900:917	normal mouse lungs	900:917	Immunostaining of normal mouse lungs with CL40 was performed and analyzed.
35889417	10	17	from	abundant	1762:1769	arg1	mesothelium					1786:1796	the pleural mesothelium	1774:1796	the pleural mesothelium	1774:1796	These results indicate that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3, under physiological conditions in mice.
35889417	0	18	theme	Sialylated	78:87	arg1	Synthesis					51:59	Synthesis	51:59	Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium	51:141	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	5	19	theme	lungs	913:917	arg1	Immunostaining					882:895	Immunostaining	882:895	Immunostaining of normal mouse lungs with CL40	882:927	Immunostaining of normal mouse lungs with CL40 was performed and analyzed.
35889417	4	20	theme	sialyl	754:759	arg1	LacNAc					769:774	sialyl 6-sulfo LacNAc	754:774	sialyl 6-sulfo LacNAc	754:774	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	0	21	theme	Sulfated	93:100	arg1	Glycans					102:108	Sulfated Glycans	93:108	Sulfated Glycans	93:108	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	10	22	from	mesothelium	1786:1796	arg1	abundant					1762:1769	abundant	1762:1769	abundant	1762:1769	These results indicate that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3, under physiological conditions in mice.
35889417	1	23	theme	secondary	344:352	arg1	organs					363:368	secondary lymphoid organs	344:368	secondary lymphoid organs	344:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	0	24	theme	GlcNAc6ST3	37:46	arg1	Role					14:17	Complementary Role	0:17	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.	0:142	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	2	25	theme	1-extended	419:428	arg1	glycans					439:445	the core 1-extended O-linked glycans	410:445	the core 1-extended O-linked glycans detected by the MECA-79 antibody	410:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	2	26	link	O-linked	430:437	arg1	glycans					439:445	the core 1-extended O-linked glycans	410:445	the core 1-extended O-linked glycans detected by the MECA-79 antibody	410:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	3	27	from	components	606:615	arg1	tissues					646:652	pulmonary tissues	636:652	pulmonary tissues	636:652	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions remain to be analyzed.
35889417	0	28	theme	Complementary	0:12	arg1	Role					14:17	Complementary Role	0:17	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.	0:142	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	1	29	theme	sulfated	264:271	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	29	theme	sulfated	264:271	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	29	theme	sulfated	264:271	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	30	located	found	296:300	arg1	HEVs					335:338	HEVs	335:338	HEVs	335:338	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	30	located	found	296:300	arg2	sialomucins					284:294	sialomucins	284:294	sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	284:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	30	located	found	296:300	arg1	venules					326:332	the high endothelial venules	305:332	the high endothelial venules (HEVs) of secondary lymphoid organs	305:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	2	31	theme	present	399:405	arg1	sLeX					394:397	6-sulfo sLeX	386:397	6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody	386:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	2	32	theme	6-sulfo	386:392	arg1	sLeX					394:397	6-sulfo sLeX	386:397	6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody	386:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	6	33	theme	epitope	1048:1054	arg1	synthesis					1026:1034	the synthesis	1022:1034	the synthesis of the CL40 epitope in the lungs	1022:1067	The contribution of GlcNAc-6-O-sulfotransferases (GlcNAc6STs) to the synthesis of the CL40 epitope in the lungs was also elucidated.
35889417	3	34	theme	6-sulfo	620:626	arg1	sLeX					628:631	6-sulfo sLeX	620:631	6-sulfo sLeX in pulmonary tissues under physiological conditions	620:683	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions remain to be analyzed.
35889417	4	35	theme	absolute	815:822	arg1	requirements					824:835	absolute requirements	815:835	absolute requirements for both GlcNAc-6-sulfation and sialylation	815:879	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	7	36	theme	CL40	1131:1134	arg1	epitope					1136:1142	the CL40 epitope	1127:1142	the CL40 epitope	1127:1142	Here, we show that the expression of the CL40 epitope was specifically detected in the mesothelin-positive mesothelium of the pulmonary pleura.
35889417	9	37	theme	epitope	1558:1564	arg1	expression					1535:1544	in vivo expression	1527:1544	in vivo expression of the CL40 epitope in the lung mesothelium	1527:1588	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	3	38	theme	physiological	660:672	arg1	conditions					674:683	physiological conditions	660:683	physiological conditions	660:683	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions remain to be analyzed.
35889417	4	39	theme	O-linked	779:786	arg1	glycans					801:807	O-linked and N-linked glycans	779:807	O-linked and N-linked glycans	779:807	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	1	40	theme	6-sulfo	208:214	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	40	theme	6-sulfo	208:214	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	40	theme	6-sulfo	208:214	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	41	theme	6-sulfo	151:157	arg1	sLeX					176:179	6-sulfo sLeX	168:179	6-sulfo sLeX	168:179	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	41	theme	6-sulfo	151:157	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	41	theme	6-sulfo	151:157	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	41	theme	6-sulfo	151:157	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	0	42	from	Mesothelium	131:141	arg1	Synthesis					51:59	Synthesis	51:59	Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium	51:141	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	10	43	theme	physiological	1870:1882	arg1	conditions					1884:1893	physiological conditions	1870:1893	physiological conditions in mice	1870:1901	These results indicate that CL40-positive sialylated and sulfated glycans are abundant in the pleural mesothelium and are synthesized complementarily by GlcNAc6ST2 and GlcNAc6ST3, under physiological conditions in mice.
35889417	4	44	theme	N-linked	792:799	arg1	glycans					801:807	O-linked and N-linked glycans	779:807	O-linked and N-linked glycans	779:807	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	8	45	dep	GlcNAc6ST3	1278:1287	arg1	encoded					1290:1296	encoded	1290:1296	encoded by Chst5	1290:1305	Moreover, GlcNAc6ST2 (encoded by Chst4) and GlcNAc6ST3 (encoded by Chst5), but not GlcNAc6ST1 (encoded by Chst2) or GlcNAc6ST4 (encoded by Chst7), are required for the synthesis of CL40-positive glycans in the lung mesothelium.
35889417	2	46	from	present	399:405	arg1	glycans					439:445	the core 1-extended O-linked glycans	410:445	the core 1-extended O-linked glycans detected by the MECA-79 antibody	410:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	1	47	theme	sialylated	249:258	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	47	theme	sialylated	249:258	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	47	theme	sialylated	249:258	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	5	48	theme	mouse	907:911	arg1	lungs					913:917	normal mouse lungs	900:917	normal mouse lungs	900:917	Immunostaining of normal mouse lungs with CL40 was performed and analyzed.
35889417	3	49	from	sLeX	628:631	arg1	tissues					646:652	pulmonary tissues	636:652	pulmonary tissues	636:652	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions remain to be analyzed.
35889417	7	50	theme	pleura	1226:1231	arg1	mesothelium					1197:1207	the mesothelin-positive mesothelium	1173:1207	the mesothelin-positive mesothelium of the pulmonary pleura	1173:1231	Here, we show that the expression of the CL40 epitope was specifically detected in the mesothelin-positive mesothelium of the pulmonary pleura.
35889417	2	51	theme	lungs	595:599	arg1	vasculature					536:546	the lymphoid aggregate vasculature	513:546	the lymphoid aggregate vasculature	513:546	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	2	51	theme	lungs	595:599	arg1	mucosa					562:567	bronchial mucosa	552:567	bronchial mucosa	552:567	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	0	52	theme	Pleural	123:129	arg1	Mesothelium					131:141	the Mouse Pleural Mesothelium	113:141	the Mouse Pleural Mesothelium	113:141	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	2	53	theme	lymphoid	517:524	arg1	vasculature					536:546	the lymphoid aggregate vasculature	513:546	the lymphoid aggregate vasculature	513:546	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	9	54	theme	GlcNAc6ST1/2/4	1646:1659	arg1	mice					1678:1681	GlcNAc6ST1/2/4 triple-knock-out mice	1646:1681	GlcNAc6ST1/2/4 triple-knock-out mice	1646:1681	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	1	55	theme	derivative	190:199	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	55	theme	derivative	190:199	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	55	theme	derivative	190:199	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	9	56	theme	triple-knock-out	1661:1676	arg1	mice					1678:1681	GlcNAc6ST1/2/4 triple-knock-out mice	1646:1681	GlcNAc6ST1/2/4 triple-knock-out mice	1646:1681	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	2	57	theme	bronchial	552:560	arg1	mucosa					562:567	bronchial mucosa	552:567	bronchial mucosa	552:567	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	8	58	theme	CL40-positive	1415:1427	arg1	glycans					1429:1435	CL40-positive glycans	1415:1435	CL40-positive glycans	1415:1435	Moreover, GlcNAc6ST2 (encoded by Chst4) and GlcNAc6ST3 (encoded by Chst5), but not GlcNAc6ST1 (encoded by Chst2) or GlcNAc6ST4 (encoded by Chst7), are required for the synthesis of CL40-positive glycans in the lung mesothelium.
35889417	9	59	from	expression	1535:1544	arg1	mesothelium					1578:1588	the lung mesothelium	1569:1588	the lung mesothelium	1569:1588	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	6	60	theme	CL40	1043:1046	arg1	epitope					1048:1054	the CL40 epitope	1039:1054	the CL40 epitope	1039:1054	The contribution of GlcNAc-6-O-sulfotransferases (GlcNAc6STs) to the synthesis of the CL40 epitope in the lungs was also elucidated.
35889417	8	61	from	synthesis	1402:1410	arg1	mesothelium					1449:1459	the lung mesothelium	1440:1459	the lung mesothelium	1440:1459	Moreover, GlcNAc6ST2 (encoded by Chst4) and GlcNAc6ST3 (encoded by Chst5), but not GlcNAc6ST1 (encoded by Chst2) or GlcNAc6ST4 (encoded by Chst7), are required for the synthesis of CL40-positive glycans in the lung mesothelium.
35889417	1	62	theme	Sialyl	144:149	arg1	sLeX					176:179	6-sulfo sLeX	168:179	6-sulfo sLeX	168:179	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	62	theme	Sialyl	144:149	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	62	theme	Sialyl	144:149	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	62	theme	Sialyl	144:149	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	8	63	theme	lung	1444:1447	arg1	mesothelium					1449:1459	the lung mesothelium	1440:1459	the lung mesothelium	1440:1459	Moreover, GlcNAc6ST2 (encoded by Chst4) and GlcNAc6ST3 (encoded by Chst5), but not GlcNAc6ST1 (encoded by Chst2) or GlcNAc6ST4 (encoded by Chst7), are required for the synthesis of CL40-positive glycans in the lung mesothelium.
35889417	4	64	with	glycans	801:807	arg1	requirements					824:835	absolute requirements	815:835	absolute requirements for both GlcNAc-6-sulfation and sialylation	815:879	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	7	65	theme	pulmonary	1216:1224	arg1	pleura					1226:1231	the pulmonary pleura	1212:1231	the pulmonary pleura	1212:1231	Here, we show that the expression of the CL40 epitope was specifically detected in the mesothelin-positive mesothelium of the pulmonary pleura.
35889417	1	66	theme	Lewis	159:163	arg1	sLeX					176:179	6-sulfo sLeX	168:179	6-sulfo sLeX	168:179	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	66	theme	Lewis	159:163	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	66	theme	Lewis	159:163	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	66	theme	Lewis	159:163	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	0	67	from	Synthesis	51:59	arg1	Mesothelium					131:141	the Mouse Pleural Mesothelium	113:141	the Mouse Pleural Mesothelium	113:141	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	0	68	from	Role	14:17	arg1	Synthesis					51:59	Synthesis	51:59	Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium	51:141	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	6	69	from	synthesis	1026:1034	arg1	lungs					1063:1067	the lungs	1059:1067	the lungs	1059:1067	The contribution of GlcNAc-6-O-sulfotransferases (GlcNAc6STs) to the synthesis of the CL40 epitope in the lungs was also elucidated.
35889417	9	70	dep	in	1527:1528	arg1	vivo					1530:1533	vivo	1530:1533	vivo	1530:1533	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	0	71	theme	Glycans	102:108	arg1	Synthesis					51:59	Synthesis	51:59	Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium	51:141	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	2	72	from	glycans	439:445	arg1	present					399:405	present	399:405	present	399:405	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	7	73	theme	mesothelin-positive	1177:1195	arg1	mesothelium					1197:1207	the mesothelin-positive mesothelium	1173:1207	the mesothelin-positive mesothelium of the pulmonary pleura	1173:1231	Here, we show that the expression of the CL40 epitope was specifically detected in the mesothelin-positive mesothelium of the pulmonary pleura.
35889417	4	74	theme	6-sulfo	761:767	arg1	LacNAc					769:774	sialyl 6-sulfo LacNAc	754:774	sialyl 6-sulfo LacNAc	754:774	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	1	75	theme	lymphoid	354:361	arg1	organs					363:368	secondary lymphoid organs	344:368	secondary lymphoid organs	344:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	2	76	theme	core	414:417	arg1	glycans					439:445	the core 1-extended O-linked glycans	410:445	the core 1-extended O-linked glycans detected by the MECA-79 antibody	410:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	0	77	theme	GlcNAc6ST2	22:31	arg1	Role					14:17	Complementary Role	0:17	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.	0:142	Complementary Role of GlcNAc6ST2 and GlcNAc6ST3 in Synthesis of CL40-Reactive Sialylated and Sulfated Glycans in the Mouse Pleural Mesothelium.
35889417	1	78	gly	sialylated	249:258	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	78	gly	sialylated	249:258	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	78	gly	sialylated	249:258	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	8	79	dep	GlcNAc6ST2	1244:1253	arg1	encoded					1256:1262	encoded	1256:1262	encoded by Chst4	1256:1271	Moreover, GlcNAc6ST2 (encoded by Chst4) and GlcNAc6ST3 (encoded by Chst5), but not GlcNAc6ST1 (encoded by Chst2) or GlcNAc6ST4 (encoded by Chst7), are required for the synthesis of CL40-positive glycans in the lung mesothelium.
35889417	4	80	link	N-linked	792:799	arg1	glycans					801:807	O-linked and N-linked glycans	779:807	O-linked and N-linked glycans	779:807	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	3	81	theme	sLeX	628:631	arg1	components					606:615	The components	602:615	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions	602:683	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions remain to be analyzed.
35889417	4	82	theme	6-sulfo	737:743	arg1	sLeX					745:748	6-sulfo sLeX	737:748	6-sulfo sLeX	737:748	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	2	83	theme	sLeX	394:397	arg1	component					373:381	A component	371:381	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody	371:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	7	84	theme	epitope	1136:1142	arg1	expression					1113:1122	the expression	1109:1122	the expression of the CL40 epitope	1109:1142	Here, we show that the expression of the CL40 epitope was specifically detected in the mesothelin-positive mesothelium of the pulmonary pleura.
35889417	1	85	theme	sialomucins	284:294	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	85	theme	sialomucins	284:294	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	85	theme	sialomucins	284:294	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	2	86	attach	present	399:405	arg1	glycans					439:445	the core 1-extended O-linked glycans	410:445	the core 1-extended O-linked glycans detected by the MECA-79 antibody	410:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	2	86	attach	present	399:405	arg2	sLeX					394:397	6-sulfo sLeX	386:397	6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody	386:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	4	87	link	O-linked	779:786	arg1	glycans					801:807	O-linked and N-linked glycans	779:807	O-linked and N-linked glycans	779:807	The CL40 antibody recognizes 6-sulfo sLeX and sialyl 6-sulfo LacNAc in O-linked and N-linked glycans, with absolute requirements for both GlcNAc-6-sulfation and sialylation.
35889417	9	88	theme	CL40	1553:1556	arg1	epitope					1558:1564	the CL40 epitope	1549:1564	the CL40 epitope	1549:1564	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	2	89	theme	O-linked	430:437	arg1	glycans					439:445	the core 1-extended O-linked glycans	410:445	the core 1-extended O-linked glycans detected by the MECA-79 antibody	410:478	A component of 6-sulfo sLeX present in the core 1-extended O-linked glycans detected by the MECA-79 antibody was previously shown to exist in the lymphoid aggregate vasculature and bronchial mucosa of allergic and asthmatic lungs.
35889417	5	90	with	Immunostaining	882:895	arg1	CL40					924:927	CL40	924:927	CL40	924:927	Immunostaining of normal mouse lungs with CL40 was performed and analyzed.
35889417	1	91	theme	high	309:312	arg1	HEVs					335:338	HEVs	335:338	HEVs	335:338	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	91	theme	high	309:312	arg1	venules					326:332	the high endothelial venules	305:332	the high endothelial venules (HEVs) of secondary lymphoid organs	305:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	3	92	theme	pulmonary	636:644	arg1	tissues					646:652	pulmonary tissues	636:652	pulmonary tissues	636:652	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions remain to be analyzed.
35889417	1	93	theme	N-acetyllactosamine	216:234	arg1	LacNAc					237:242	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc)	186:243	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	93	theme	N-acetyllactosamine	216:234	arg1	glycans					273:279	sialylated and sulfated glycans	249:279	sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs	249:368	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	1	93	theme	N-acetyllactosamine	216:234	arg1	X					165:165	Sialyl 6-sulfo Lewis X	144:165	Sialyl 6-sulfo Lewis X (6-sulfo sLeX)	144:180	Sialyl 6-sulfo Lewis X (6-sulfo sLeX) and its derivative sialyl 6-sulfo N-acetyllactosamine (LacNAc) are sialylated and sulfated glycans of sialomucins found in the high endothelial venules (HEVs) of secondary lymphoid organs.
35889417	9	94	theme	lung	1573:1576	arg1	mesothelium					1578:1588	the lung mesothelium	1569:1588	the lung mesothelium	1569:1588	Furthermore, neither GlcNAc6ST2 nor GlcNAc6ST3 is sufficient for in vivo expression of the CL40 epitope in the lung mesothelium, as demonstrated by GlcNAc6ST1/3/4 triple-knock-out and GlcNAc6ST1/2/4 triple-knock-out mice.
35889417	3	95	from	tissues	646:652	arg1	components					606:615	The components	602:615	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions	602:683	The components of 6-sulfo sLeX in pulmonary tissues under physiological conditions remain to be analyzed.
37164255	10	0	theme	MPO	1656:1658	arg1	levels					1646:1651	LPS-induced expression levels	1623:1651	LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS)	1623:1723	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	1	1	theme	classic	270:276	arg1	medicine					298:305	the classic traditional Chinese medicine	266:305	the classic traditional Chinese medicine prescription Yu Ping Feng San	266:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	10	2	theme	factors	1686:1692	arg1	levels					1646:1651	LPS-induced expression levels	1623:1651	LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS)	1623:1723	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	1	3	theme	Chinese	290:296	arg1	medicine					298:305	the classic traditional Chinese medicine	266:305	the classic traditional Chinese medicine prescription Yu Ping Feng San	266:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	12	4	theme	signaling	1891:1899	arg1	p-IκB					1943:1947	p-IκB	1943:1947	p-IκB	1943:1947	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	4	theme	signaling	1891:1899	arg1	p-p65					1954:1958	p-p65	1954:1958	p-p65	1954:1958	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	4	theme	signaling	1891:1899	arg1	Myd88					1936:1940	Myd88	1936:1940	Myd88	1936:1940	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	4	theme	signaling	1891:1899	arg1	TLR4					1930:1933	TLR4	1930:1933	TLR4	1930:1933	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	4	theme	signaling	1891:1899	arg1	proteins					1920:1927	TLR4/NF-κB signaling pathway-associated proteins	1880:1927	TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65)	1880:1959	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	9	5	theme	same	1385:1388	arg1	time					1390:1393	the same time	1381:1393	the same time	1381:1393	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	12	6	theme	proteins	1920:1927	arg1	level					1871:1875	the expression level	1856:1875	the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β)	1856:2025	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	6	7	theme	THP-1 cell	1076:1085	arg1	model					1100:1104	a THP-1 cell inflammation model	1074:1104	a THP-1 cell inflammation model	1074:1104	Finally, the molecular mechanism of SWQ was validated using a rat ALI model and a THP-1 cell inflammation model.
37164255	8	8	theme	weight	1305:1310	arg1	ratio					1312:1316	lung wet/dry weight ratio	1292:1316	lung wet/dry weight ratio (W/D)	1292:1322	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	8	8	theme	weight	1305:1310	arg1	W/D					1319:1321	W/D	1319:1321	W/D	1319:1321	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	0	9	theme	-induced	141:148	arg1	injury					161:166	lipopolysaccharide -induced acute lung injury	122:166	lipopolysaccharide -induced acute lung injury	122:166	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	3	10	theme	preventive	511:520	arg1	effects					538:544	the preventive and therapeutic effects	507:544	the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI)	507:611	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	12	11	theme	expression	1860:1869	arg1	level					1871:1875	the expression level	1856:1875	the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β)	1856:2025	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	10	12	theme	white	1560:1564	arg1	count					1594:1598	white blood cells and neutrophils count	1560:1598	white blood cells and neutrophils count in BALF	1560:1606	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	11	13	theme	Network	1726:1732	arg1	pharmacology					1734:1745	Network pharmacology	1726:1745	Network pharmacology	1726:1745	Network pharmacology showed that SWQ was associated with TLR4/NF-κB inflammation pathway.
37164255	0	14	theme	lung	156:159	arg1	injury					161:166	lipopolysaccharide -induced acute lung injury	122:166	lipopolysaccharide -induced acute lung injury	122:166	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	1	15	theme	Chinese	234:240	arg1	RELEVANCE					190:198	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	1	15	theme	Chinese	234:240	arg1	formula					249:255	a Chinese herbal formula	232:255	a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San	232:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	3	16	theme	acute	589:593	arg1	ALI					608:610	ALI	608:610	ALI	608:610	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	3	16	theme	acute	589:593	arg1	injury					600:605	acute lung injury	589:605	lipopolysaccharide (LPS)-induced acute lung injury (ALI)	556:611	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	13	17	theme	inflammasome	2164:2175	arg1	activation					2177:2186	NLRP3 inflammasome activation	2158:2186	NLRP3 inflammasome activation	2158:2186	CONCLUSIONS The present study demonstrates that SWQ can reduce inflammation in ALI by inhibiting TLR4/NF-κB and NLRP3 inflammasome activation.
37164255	9	18	theme	mediators	1438:1446	arg1	penetration					1410:1420	penetration	1410:1420	penetration of inflammatory mediators	1410:1446	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	0	19	theme	NLRP3	68:72	arg1	inflammasome					74:85	NLRP3 inflammasome	68:85	NLRP3 inflammasome	68:85	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	4	20	dep	determined	764:773	arg1	MATERIALS					685:693	MATERIALS	685:693	MATERIALS	685:693	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	4	20	dep	determined	764:773	arg1	First					707:711	First	707:711	First	707:711	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	4	20	dep	determined	764:773	arg1	METHODS					699:705	METHODS	699:705	METHODS	699:705	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	12	21	theme	NLRP3	1965:1969	arg1	inflammasome					1971:1982	NLRP3 inflammasome	1965:1982	NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β)	1965:2025	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	1	22	theme	Shiwei	200:205	arg1	SWQ					226:228	SWQ	226:228	SWQ	226:228	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	1	22	theme	Shiwei	200:205	arg1	decoction					215:223	Shiwei Qingwen decoction	200:223	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	4	23	theme	LPS-induced	895:905	arg1	model					915:919	the LPS-induced ALI rat model	891:919	the LPS-induced ALI rat model	891:919	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	1	24	dep	prescription	307:318	arg1	San					333:335	San	333:335	San	333:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	0	25	theme	inflammatory	97:108	arg1	response					110:117	inflammatory response	97:117	inflammatory response in lipopolysaccharide -induced acute lung injury	97:166	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	4	26	theme	rat	911:913	arg1	model					915:919	the LPS-induced ALI rat model	891:919	the LPS-induced ALI rat model	891:919	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	12	27	dep	proteins	1920:1927	arg1	p-IκB					1943:1947	p-IκB	1943:1947	p-IκB	1943:1947	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	27	dep	proteins	1920:1927	arg1	p-p65					1954:1958	p-p65	1954:1958	p-p65	1954:1958	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	27	dep	proteins	1920:1927	arg1	Myd88					1936:1940	Myd88	1936:1940	Myd88	1936:1940	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	27	dep	proteins	1920:1927	arg1	TLR4					1930:1933	TLR4	1930:1933	TLR4	1930:1933	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	27	dep	proteins	1920:1927	arg1	proteins					1920:1927	TLR4/NF-κB signaling pathway-associated proteins	1880:1927	TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65)	1880:1959	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	8	28	theme	wet/dry	1297:1303	arg1	ratio					1312:1316	lung wet/dry weight ratio	1292:1316	lung wet/dry weight ratio (W/D)	1292:1322	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	8	28	theme	wet/dry	1297:1303	arg1	W/D					1319:1321	W/D	1319:1321	W/D	1319:1321	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	1	29	theme	early	384:388	arg1	pneumonia					390:398	early pneumonia	384:398	early pneumonia with good clinical outcomes	384:426	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	9	30	theme	nitric	1494:1499	arg1	synthase					1507:1514	endothelial nitric oxide synthase	1482:1514	endothelial nitric oxide synthase (eNOS)	1482:1521	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	9	30	theme	nitric	1494:1499	arg1	eNOS					1517:1520	eNOS	1517:1520	eNOS	1517:1520	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	1	31	dep	RELEVANCE	190:198	arg1	SWQ					226:228	SWQ	226:228	SWQ	226:228	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	1	31	dep	RELEVANCE	190:198	arg1	decoction					215:223	Shiwei Qingwen decoction	200:223	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	0	32	theme	Shiwei	0:5	arg1	decoction					15:23	Shiwei Qingwen decoction	0:23	Shiwei Qingwen decoction	0:23	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	1	33	theme	medicine	298:305	arg1	prescription					307:318	the classic traditional Chinese medicine prescription Yu Ping Feng San	266:335	the classic traditional Chinese medicine prescription Yu Ping Feng San	266:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	6	34	theme	molecular	1007:1015	arg1	mechanism					1017:1025	the molecular mechanism	1003:1025	the molecular mechanism of SWQ	1003:1032	Finally, the molecular mechanism of SWQ was validated using a rat ALI model and a THP-1 cell inflammation model.
37164255	4	35	theme	SWQ	742:744	arg1	composition					727:737	the chemical composition	714:737	the chemical composition of SWQ	714:744	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	7	36	theme	RESULTS	1107:1113	arg1	HPLC					1115:1118	RESULTS HPLC	1107:1118	RESULTS HPLC	1107:1118	RESULTS HPLC identified chlorogenic acid, prime-O-glucosylcimifugin, calycosin, and 5-O-methylaminoside in the chemical profile of SWQ.
37164255	12	37	dep	inflammasome	1971:1982	arg1	caspase-1					1997:2005	caspase-1	1997:2005	caspase-1	1997:2005	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	37	dep	inflammasome	1971:1982	arg1	cleaved-IL-1β					2012:2024	cleaved-IL-1β	2012:2024	cleaved-IL-1β	2012:2024	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	37	dep	inflammasome	1971:1982	arg1	ASC					1992:1994	ASC	1992:1994	ASC	1992:1994	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	37	dep	inflammasome	1971:1982	arg1	NLRP3					1985:1989	NLRP3	1985:1989	NLRP3	1985:1989	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	0	38	theme	TLR4/NF-κB	35:44	arg1	pathway					56:62	TLR4/NF-κB signaling pathway	35:62	TLR4/NF-κB signaling pathway	35:62	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	8	39	theme	histopathological	1339:1355	arg1	damage					1357:1362	histopathological damage	1339:1362	histopathological damage to the lungs	1339:1375	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	4	40	dep	SWQ	843:845	arg1	6.55					854:857	6.55	854:857	6.55	854:857	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	4	40	dep	SWQ	843:845	arg1	13.1 g/kg					864:872	13.1 g/kg	864:872	13.1 g/kg	864:872	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	4	40	dep	SWQ	843:845	arg1	3.27					848:851	3.27	848:851	3.27	848:851	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	10	41	from	count	1594:1598	arg1	BALF					1603:1606	BALF	1603:1606	BALF	1603:1606	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	4	42	theme	performance	783:793	arg1	HPLC					818:821	HPLC	818:821	HPLC	818:821	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	4	42	theme	performance	783:793	arg1	chromatography					802:815	high performance liquid chromatography	778:815	high performance liquid chromatography (HPLC)	778:822	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	10	43	theme	expression	1635:1644	arg1	levels					1646:1651	LPS-induced expression levels	1623:1651	LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS)	1623:1723	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	1	44	theme	herbal	242:247	arg1	RELEVANCE					190:198	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	1	44	theme	herbal	242:247	arg1	formula					249:255	a Chinese herbal formula	232:255	a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San	232:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	5	45	theme	inflammatory	932:943	arg1	pathway					945:951	its inflammatory pathway	928:951	its inflammatory pathway	928:951	Next, its inflammatory pathway was determined via network pharmacology.
37164255	6	46	theme	rat	1056:1058	arg1	model					1064:1068	a rat ALI model	1054:1068	a rat ALI model	1054:1068	Finally, the molecular mechanism of SWQ was validated using a rat ALI model and a THP-1 cell inflammation model.
37164255	11	47	theme	TLR4/NF-κB	1783:1792	arg1	pathway					1807:1813	TLR4/NF-κB inflammation pathway	1783:1813	TLR4/NF-κB inflammation pathway	1783:1813	Network pharmacology showed that SWQ was associated with TLR4/NF-κB inflammation pathway.
37164255	13	48	theme	present	2062:2068	arg1	study					2070:2074	The present study	2058:2074	The present study	2058:2074	CONCLUSIONS The present study demonstrates that SWQ can reduce inflammation in ALI by inhibiting TLR4/NF-κB and NLRP3 inflammasome activation.
37164255	7	49	theme	chemical	1218:1225	arg1	profile					1227:1233	the chemical profile	1214:1233	the chemical profile of SWQ	1214:1240	RESULTS HPLC identified chlorogenic acid, prime-O-glucosylcimifugin, calycosin, and 5-O-methylaminoside in the chemical profile of SWQ.
37164255	1	50	dep	good	405:408	arg1	clinical					410:417	clinical	410:417	clinical	410:417	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	4	51	theme	SWQ	843:845	arg1	impact					833:838	the impact	829:838	the impact of SWQ (3.27, 6.55, and 13.1 g/kg)	829:873	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	3	52	theme	SWQ	549:551	arg1	effects					538:544	the preventive and therapeutic effects	507:544	the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI)	507:611	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	1	53	theme	traditional	278:288	arg1	medicine					298:305	the classic traditional Chinese medicine	266:305	the classic traditional Chinese medicine prescription Yu Ping Feng San	266:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	10	54	theme	pro-inflammatory	1669:1684	arg1	factors					1686:1692	pro-inflammatory factors	1669:1692	pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS)	1669:1723	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	54	theme	pro-inflammatory	1669:1684	arg1	IL-6					1709:1712	IL-6	1709:1712	IL-6	1709:1712	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	54	theme	pro-inflammatory	1669:1684	arg1	iNOS					1719:1722	iNOS	1719:1722	iNOS	1719:1722	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	54	theme	pro-inflammatory	1669:1684	arg1	IL-1β					1702:1706	IL-1β	1702:1706	IL-1β	1702:1706	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	54	theme	pro-inflammatory	1669:1684	arg1	TNF-α					1695:1699	TNF-α	1695:1699	TNF-α	1695:1699	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	1	55	theme	Qingwen	207:213	arg1	SWQ					226:228	SWQ	226:228	SWQ	226:228	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	1	55	theme	Qingwen	207:213	arg1	decoction					215:223	Shiwei Qingwen decoction	200:223	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	12	56	theme	TLR4/NF-κB	1880:1889	arg1	p-IκB					1943:1947	p-IκB	1943:1947	p-IκB	1943:1947	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	56	theme	TLR4/NF-κB	1880:1889	arg1	p-p65					1954:1958	p-p65	1954:1958	p-p65	1954:1958	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	56	theme	TLR4/NF-κB	1880:1889	arg1	Myd88					1936:1940	Myd88	1936:1940	Myd88	1936:1940	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	56	theme	TLR4/NF-κB	1880:1889	arg1	TLR4					1930:1933	TLR4	1930:1933	TLR4	1930:1933	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	56	theme	TLR4/NF-κB	1880:1889	arg1	proteins					1920:1927	TLR4/NF-κB signaling pathway-associated proteins	1880:1927	TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65)	1880:1959	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	6	57	theme	inflammation	1087:1098	arg1	model					1100:1104	a THP-1 cell inflammation model	1074:1104	a THP-1 cell inflammation model	1074:1104	Finally, the molecular mechanism of SWQ was validated using a rat ALI model and a THP-1 cell inflammation model.
37164255	0	58	theme	acute	150:154	arg1	injury					161:166	lipopolysaccharide -induced acute lung injury	122:166	lipopolysaccharide -induced acute lung injury	122:166	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	12	59	theme	pathway-associated	1901:1918	arg1	p-IκB					1943:1947	p-IκB	1943:1947	p-IκB	1943:1947	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	59	theme	pathway-associated	1901:1918	arg1	p-p65					1954:1958	p-p65	1954:1958	p-p65	1954:1958	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	59	theme	pathway-associated	1901:1918	arg1	Myd88					1936:1940	Myd88	1936:1940	Myd88	1936:1940	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	59	theme	pathway-associated	1901:1918	arg1	TLR4					1930:1933	TLR4	1930:1933	TLR4	1930:1933	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	12	59	theme	pathway-associated	1901:1918	arg1	proteins					1920:1927	TLR4/NF-κB signaling pathway-associated proteins	1880:1927	TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65)	1880:1959	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	10	60	theme	blood	1566:1570	arg1	cells					1572:1576	blood cells	1566:1576	blood cells	1566:1576	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	1	61	theme	good	405:408	arg1	outcomes					419:426	good clinical outcomes	405:426	good clinical outcomes	405:426	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	3	62	theme	lung	595:598	arg1	ALI					608:610	ALI	608:610	ALI	608:610	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	3	62	theme	lung	595:598	arg1	injury					600:605	acute lung injury	589:605	lipopolysaccharide (LPS)-induced acute lung injury (ALI)	556:611	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	13	63	dep	CONCLUSIONS	2046:2056	arg1	demonstrates					2076:2087	demonstrates	2076:2087	demonstrates that SWQ can reduce inflammation in ALI by inhibiting TLR4/NF-κB and NLRP3 inflammasome activation	2076:2186	CONCLUSIONS The present study demonstrates that SWQ can reduce inflammation in ALI by inhibiting TLR4/NF-κB and NLRP3 inflammasome activation.
37164255	3	64	theme	-induced	580:587	arg1	ALI					608:610	ALI	608:610	ALI	608:610	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	3	64	theme	-induced	580:587	arg1	injury					600:605	acute lung injury	589:605	lipopolysaccharide (LPS)-induced acute lung injury (ALI)	556:611	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	13	65	theme	NLRP3	2158:2162	arg1	activation					2177:2186	NLRP3 inflammasome activation	2158:2186	NLRP3 inflammasome activation	2158:2186	CONCLUSIONS The present study demonstrates that SWQ can reduce inflammation in ALI by inhibiting TLR4/NF-κB and NLRP3 inflammasome activation.
37164255	10	66	theme	NE	1661:1662	arg1	levels					1646:1651	LPS-induced expression levels	1623:1651	LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS)	1623:1723	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	12	67	theme	inflammasome	1971:1982	arg1	level					1871:1875	the expression level	1856:1875	the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β)	1856:2025	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	9	68	theme	inflammatory	1425:1436	arg1	mediators					1438:1446	inflammatory mediators	1425:1446	inflammatory mediators	1425:1446	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	1	69	with	pneumonia	390:398	arg1	outcomes					419:426	good clinical outcomes	405:426	good clinical outcomes	405:426	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	0	70	from	response	110:117	arg1	injury					161:166	lipopolysaccharide -induced acute lung injury	122:166	lipopolysaccharide -induced acute lung injury	122:166	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	8	71	theme	ALI	1250:1252	arg1	model					1254:1258	the ALI model	1246:1258	the ALI model	1246:1258	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	3	72	theme	STUDY	490:494	arg1	AIM					479:481	AIM	479:481	AIM OF THE STUDY	479:494	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	3	73	from	effects	538:544	arg1	ALI					608:610	ALI	608:610	ALI	608:610	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	3	73	from	effects	538:544	arg1	injury					600:605	acute lung injury	589:605	lipopolysaccharide (LPS)-induced acute lung injury (ALI)	556:611	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	10	74	dep	factors	1686:1692	arg1	factors					1686:1692	pro-inflammatory factors	1669:1692	pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS)	1669:1723	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	74	dep	factors	1686:1692	arg1	IL-6					1709:1712	IL-6	1709:1712	IL-6	1709:1712	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	74	dep	factors	1686:1692	arg1	iNOS					1719:1722	iNOS	1719:1722	iNOS	1719:1722	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	74	dep	factors	1686:1692	arg1	IL-1β					1702:1706	IL-1β	1702:1706	IL-1β	1702:1706	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	74	dep	factors	1686:1692	arg1	TNF-α					1695:1699	TNF-α	1695:1699	TNF-α	1695:1699	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	10	75	theme	LPS-induced	1623:1633	arg1	levels					1646:1651	LPS-induced expression levels	1623:1651	LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS)	1623:1723	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	7	76	theme	chlorogenic	1131:1141	arg1	acid					1143:1146	chlorogenic acid	1131:1146	chlorogenic acid	1131:1146	RESULTS HPLC identified chlorogenic acid, prime-O-glucosylcimifugin, calycosin, and 5-O-methylaminoside in the chemical profile of SWQ.
37164255	9	77	theme	endothelial	1482:1492	arg1	synthase					1507:1514	endothelial nitric oxide synthase	1482:1514	endothelial nitric oxide synthase (eNOS)	1482:1521	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	9	77	theme	endothelial	1482:1492	arg1	eNOS					1517:1520	eNOS	1517:1520	eNOS	1517:1520	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	6	78	theme	SWQ	1030:1032	arg1	mechanism					1017:1025	the molecular mechanism	1003:1025	the molecular mechanism of SWQ	1003:1032	Finally, the molecular mechanism of SWQ was validated using a rat ALI model and a THP-1 cell inflammation model.
37164255	4	79	theme	ALI	907:909	arg1	model					915:919	the LPS-induced ALI rat model	891:919	the LPS-induced ALI rat model	891:919	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	8	80	theme	lung	1292:1295	arg1	ratio					1312:1316	lung wet/dry weight ratio	1292:1316	lung wet/dry weight ratio (W/D)	1292:1322	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	8	80	theme	lung	1292:1295	arg1	W/D					1319:1321	W/D	1319:1321	W/D	1319:1321	In the ALI model, SWQ alleviated ALI by reducing lung wet/dry weight ratio (W/D) and preventing histopathological damage to the lungs.
37164255	10	81	theme	neutrophils	1582:1592	arg1	count					1594:1598	white blood cells and neutrophils count	1560:1598	white blood cells and neutrophils count in BALF	1560:1606	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	0	82	theme	Qingwen	7:13	arg1	decoction					15:23	Shiwei Qingwen decoction	0:23	Shiwei Qingwen decoction	0:23	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	9	83	theme	oxide	1501:1505	arg1	synthase					1507:1514	endothelial nitric oxide synthase	1482:1514	endothelial nitric oxide synthase (eNOS)	1482:1521	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	9	83	theme	oxide	1501:1505	arg1	eNOS					1517:1520	eNOS	1517:1520	eNOS	1517:1520	At the same time, SWQ decreased penetration of inflammatory mediators by upregulating AQP1 and AQP5 and endothelial nitric oxide synthase (eNOS).
37164255	4	84	theme	chemical	718:725	arg1	composition					727:737	the chemical composition	714:737	the chemical composition of SWQ	714:744	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	10	85	theme	cells	1572:1576	arg1	count					1594:1598	white blood cells and neutrophils count	1560:1598	white blood cells and neutrophils count in BALF	1560:1606	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	2	86	theme	underlying	442:451	arg1	mechanism					453:461	its underlying mechanism	438:461	its underlying mechanism	438:461	However, its underlying mechanism is yet unclear.
37164255	3	87	theme	underlying	629:638	arg1	mechanism					640:648	the underlying mechanism	625:648	the underlying mechanism by which SWQ influences pneumonia	625:682	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	0	88	theme	signaling	46:54	arg1	pathway					56:62	TLR4/NF-κB signaling pathway	35:62	TLR4/NF-κB signaling pathway	35:62	Shiwei Qingwen decoction regulates TLR4/NF-κB signaling pathway and NLRP3 inflammasome to reduce inflammatory response in lipopolysaccharide -induced acute lung injury.
37164255	12	89	with	pretreatment	1826:1837	arg1	SWQ					1844:1846	SWQ	1844:1846	SWQ	1844:1846	Moreover, pretreatment with SWQ reduced the expression level of TLR4/NF-κB signaling pathway-associated proteins (TLR4, Myd88, p-IκB, and p-p65) and NLRP3 inflammasome (NLRP3, ASC, caspase-1, and cleaved-IL-1β) in vivo and vitro.
37164255	5	90	theme	network	972:978	arg1	pharmacology					980:991	network pharmacology	972:991	network pharmacology	972:991	Next, its inflammatory pathway was determined via network pharmacology.
37164255	7	91	theme	SWQ	1238:1240	arg1	profile					1227:1233	the chemical profile	1214:1233	the chemical profile of SWQ	1214:1240	RESULTS HPLC identified chlorogenic acid, prime-O-glucosylcimifugin, calycosin, and 5-O-methylaminoside in the chemical profile of SWQ.
37164255	1	92	theme	ETHNOPHARMACOLOGICAL	169:188	arg1	RELEVANCE					190:198	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ)	169:229	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	1	92	theme	ETHNOPHARMACOLOGICAL	169:188	arg1	formula					249:255	a Chinese herbal formula	232:255	a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San	232:335	ETHNOPHARMACOLOGICAL RELEVANCE Shiwei Qingwen decoction (SWQ), a Chinese herbal formula based on the classic traditional Chinese medicine prescription Yu Ping Feng San, has shown efficacy in preventing and treating early pneumonia with good clinical outcomes.
37164255	10	93	with	Pretreatment	1524:1535	arg1	SWQ					1542:1544	SWQ	1542:1544	SWQ	1542:1544	Pretreatment with SWQ downregulated white blood cells and neutrophils count in BALF and suppressed LPS-induced expression levels of MPO, NE, and pro-inflammatory factors (TNF-α, IL-1β, IL-6, and iNOS).
37164255	6	94	theme	ALI	1060:1062	arg1	model					1064:1068	a rat ALI model	1054:1068	a rat ALI model	1054:1068	Finally, the molecular mechanism of SWQ was validated using a rat ALI model and a THP-1 cell inflammation model.
37164255	4	95	theme	high	778:781	arg1	HPLC					818:821	HPLC	818:821	HPLC	818:821	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	4	95	theme	high	778:781	arg1	chromatography					802:815	high performance liquid chromatography	778:815	high performance liquid chromatography (HPLC)	778:822	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	3	96	theme	therapeutic	526:536	arg1	effects					538:544	the preventive and therapeutic effects	507:544	the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI)	507:611	AIM OF THE STUDY To clarify the preventive and therapeutic effects of SWQ on lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore the underlying mechanism by which SWQ influences pneumonia.
37164255	4	97	theme	liquid	795:800	arg1	HPLC					818:821	HPLC	818:821	HPLC	818:821	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	4	97	theme	liquid	795:800	arg1	chromatography					802:815	high performance liquid chromatography	778:815	high performance liquid chromatography (HPLC)	778:822	MATERIALS AND METHODS First, the chemical composition of SWQ was preliminarily determined by high performance liquid chromatography (HPLC), and the impact of SWQ (3.27, 6.55, and 13.1 g/kg) was assessed in the LPS-induced ALI rat model.
37164255	11	98	theme	inflammation	1794:1805	arg1	pathway					1807:1813	TLR4/NF-κB inflammation pathway	1783:1813	TLR4/NF-κB inflammation pathway	1783:1813	Network pharmacology showed that SWQ was associated with TLR4/NF-κB inflammation pathway.
36264676	12	0	theme	hybridization	1711:1723	arg1	results					1662:1668	the results	1658:1668	the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses	1658:1780	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	11	1	theme	l-Orn-d-Asp	1539:1549	arg1	bridge					1564:1569	an inferred l-Orn-d-Asp interpeptide bridge	1527:1569	an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T	1527:1646	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	14	2	theme	1.2501T=KCTC	2015:2026	arg1	49675T					2028:2033	zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T	1998:2033	zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T	1998:2033	nov. (zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T) and Cellulomonas fengjieae sp.
36264676	14	2	theme	1.2501T=KCTC	2015:2026	arg1	nov					1992:1994	nov	1992:1994	nov	1992:1994	nov. (zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T) and Cellulomonas fengjieae sp.
36264676	6	3	theme	content	789:795	arg1	range					797:801	a DNA G+C content range	779:801	a DNA G+C content range of 73.0-74.6 mol%	779:819	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	7	4	theme	fatty	832:836	arg1	C16:0					920:924	C16:0	920:924	C16:0	920:924	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	7	4	theme	fatty	832:836	arg1	acids					838:842	The major fatty acids	822:842	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199	822:913	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	9	5	dep	strains	1254:1260	arg1	zg-ZUI222T					1262:1271	zg-ZUI222T	1262:1271	zg-ZUI222T	1262:1271	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	5	dep	strains	1254:1260	arg1	%					1279:1279	87.4 %	1274:1279	87.4 %	1274:1279	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	5	dep	strains	1254:1260	arg1	zg-ZUI188T					1286:1295	zg-ZUI188T	1286:1295	zg-ZUI188T	1286:1295	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	5	dep	strains	1254:1260	arg1	%					1303:1303	91.4 %	1298:1303	91.4 %	1298:1303	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	5	dep	strains	1254:1260	arg1	strains					1254:1260	strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %)	1254:1304	strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %)	1254:1304	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	6	6	theme	DNA	781:783	arg1	content					789:795	DNA G+C content	781:795	a DNA G+C content range of 73.0-74.6 mol%	779:819	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	8	7	theme	novel	1190:1194	arg1	species					1196:1202	the three novel species	1180:1202	the three novel species	1180:1202	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	7	8	from	A-C15:1	953:959	arg1	pair					1032:1035	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	5	9	theme	rRNA	516:519	arg1	gene					521:524	the 16S rRNA gene	508:524	the 16S rRNA gene	508:524	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	12	10	theme	Cellulomonas	1890:1901	arg1	sp					1917:1918	Cellulomonas dongxiuzhuiae sp	1890:1918	Cellulomonas dongxiuzhuiae sp	1890:1918	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	6	11	theme	type	604:607	arg1	strains					609:615	The three type strains	594:615	The three type strains	594:615	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	5	12	theme	Phylogenetic	477:488	arg1	analyses					490:497	Phylogenetic analyses	477:497	Phylogenetic analyses based on the 16S rRNA gene and 247 core genes	477:543	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	15	13	theme	1.2563T=KCTC	2090:2101	arg1	49674T					2103:2108	zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T	2073:2108	zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T	2073:2108	nov. (zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T) are proposed.
36264676	15	13	theme	1.2563T=KCTC	2090:2101	arg1	nov					2067:2069	nov	2067:2069	nov	2067:2069	nov. (zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T) are proposed.
36264676	11	14	theme	cell-wall	1457:1465	arg1	A4β					1517:1519	A4β	1517:1519	A4β	1517:1519	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	14	theme	cell-wall	1457:1465	arg1	type					1481:1484	The cell-wall peptidoglycan type	1453:1484	The cell-wall peptidoglycan type of the three novel species	1453:1511	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	9	15	theme	predominant	1222:1232	arg1	MK-9					1205:1208	MK-9	1205:1208	MK-9(H4)	1205:1212	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	15	theme	predominant	1222:1232	arg1	quinone					1234:1240	the predominant quinone	1218:1240	the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %)	1218:1304	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	6	16	contain	have	617:620	arg2	range					797:801	a DNA G+C content range	779:801	a DNA G+C content range of 73.0-74.6 mol%	779:819	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	16	contain	have	617:620	arg2	values					701:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values	622:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas	622:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	16	contain	have	617:620	arg2	%					718:718	78.0-85.5%	709:718	78.0-85.5%	709:718	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	16	contain	have	617:620	arg1	strains					609:615	The three type strains	594:615	The three type strains	594:615	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	2	17	theme	fengjieae	85:93	arg1	sp					95:96	fengjieae sp	85:96	fengjieae sp	85:96	nov. and Cellulomonas fengjieae sp.
36264676	6	18	with	values	701:706	arg1	genomes					740:746	all available genomes	726:746	all available genomes in the genus Cellulomonas	726:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	4	19	theme	urease-	244:250	arg1	bacteria					285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria	166:292	bacteria	285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	4	19	theme	urease-	244:250	arg1	zg-ZUI168					353:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	6	20	theme	G+C	785:787	arg1	content					789:795	DNA G+C content	781:795	a DNA G+C content range of 73.0-74.6 mol%	779:819	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	4	21	theme	oxidase-negative	256:271	arg1	bacteria					285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria	166:292	bacteria	285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	4	21	theme	oxidase-negative	256:271	arg1	zg-ZUI168					353:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	6	22	theme	hybridization	642:654	arg1	values					701:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values	622:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas	622:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	22	theme	hybridization	642:654	arg1	%					718:718	78.0-85.5%	709:718	78.0-85.5%	709:718	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	5	23	theme	core	534:537	arg1	genes					539:543	247 core genes	530:543	247 core genes	530:543	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	5	24	theme	strains	435:441	arg1	growth					417:422	Optimal growth	409:422	Optimal growth of the six strains	409:441	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	3	25	theme	intestinal	123:132	arg1	contents					134:141	the intestinal contents	119:141	the intestinal contents of Marmota himalayana	119:163	nov., isolated from the intestinal contents of Marmota himalayana.
36264676	6	26	theme	digital	626:632	arg1	hybridization					642:654	digital DNA-DNA hybridization	626:654	digital DNA-DNA hybridization (19.3-30.1%)	626:667	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	26	theme	digital	626:632	arg1	%					666:666	19.3-30.1%	657:666	19.3-30.1%	657:666	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	4	27	theme	catalase-positive	225:241	arg1	bacteria					285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria	166:292	bacteria	285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	4	27	theme	catalase-positive	225:241	arg1	zg-ZUI168					353:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	2	28	dep	sp	95:96	arg1	nov.					63:66	nov.	63:66	nov.	63:66	nov. and Cellulomonas fengjieae sp.
36264676	2	28	dep	sp	95:96	arg1	Cellulomonas					72:83	Cellulomonas	72:83	Cellulomonas	72:83	nov. and Cellulomonas fengjieae sp.
36264676	14	29	theme	Cellulomonas	2040:2051	arg1	sp					2063:2064	Cellulomonas fengjieae sp	2040:2064	Cellulomonas fengjieae sp	2040:2064	nov. (zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T) and Cellulomonas fengjieae sp.
36264676	12	30	theme	Cellulomonas	1848:1859	arg1	species					1861:1867	three novel Cellulomonas species	1836:1867	three novel Cellulomonas species	1836:1867	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	12	31	dep	sp	1917:1918	arg1	names					1884:1888	the names	1880:1888	the names	1880:1888	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	3	32	theme	himalayana	154:163	arg1	contents					134:141	the intestinal contents	119:141	the intestinal contents of Marmota himalayana	119:163	nov., isolated from the intestinal contents of Marmota himalayana.
36264676	13	33	theme	wangleii	1979:1986	arg1	sp					1988:1989	Cellulomonas wangleii sp	1966:1989	Cellulomonas wangleii sp	1966:1989	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	10	34	theme	cell-wall	1373:1381	arg1	sugars					1383:1388	The cell-wall sugars	1369:1388	The cell-wall sugars detected in the three novel species	1369:1424	The cell-wall sugars detected in the three novel species mainly contained rhamnose.
36264676	6	35	theme	identity	692:699	arg1	values					701:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values	622:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas	622:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	35	theme	identity	692:699	arg1	%					718:718	78.0-85.5%	709:718	78.0-85.5%	709:718	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	0	36	theme	Cellulomonas	0:11	arg1	sp					27:28	Cellulomonas dongxiuzhuiae sp	0:28	Cellulomonas dongxiuzhuiae sp.	0:29	Cellulomonas dongxiuzhuiae sp.
36264676	7	37	theme	zg-ZUI	893:898	arg1	222T/zg-ZUI199					900:913	zg-ZUI 222T/zg-ZUI199	893:913	zg-ZUI 222T/zg-ZUI199	893:913	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	4	38	theme	rod-shaped	274:283	arg1	bacteria					285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria	166:292	bacteria	285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	4	38	theme	rod-shaped	274:283	arg1	zg-ZUI168					353:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	10	39	theme	novel	1412:1416	arg1	species					1418:1424	the three novel species	1402:1424	the three novel species	1402:1424	The cell-wall sugars detected in the three novel species mainly contained rhamnose.
36264676	6	40	theme	average	673:679	arg1	identity					692:699	average nucleotide identity	673:699	average nucleotide identity	673:699	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	41	with	range	797:801	arg1	genomes					740:746	all available genomes	726:746	all available genomes in the genus Cellulomonas	726:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	7	42	theme	major	826:830	arg1	C16:0					920:924	C16:0	920:924	C16:0	920:924	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	7	42	theme	major	826:830	arg1	acids					838:842	The major fatty acids	822:842	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199	822:913	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	11	43	dep	strains	1575:1581	arg1	zg-ZUI157T					1583:1592	zg-ZUI157T	1583:1592	zg-ZUI157T	1583:1592	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	43	dep	strains	1575:1581	arg1	strains					1575:1581	strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T	1575:1646	strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T	1575:1646	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	43	dep	strains	1575:1581	arg1	zg-ZUI222T					1598:1607	zg-ZUI222T	1598:1607	zg-ZUI222T	1598:1607	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	12	44	theme	nucleotide	1734:1743	arg1	identity					1745:1752	average nucleotide identity	1726:1752	average nucleotide identity	1726:1752	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	13	45	dep	sp	1988:1989	arg1	49678T					1957:1962	zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T	1927:1962	zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T	1927:1962	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	13	45	dep	sp	1988:1989	arg1	nov					1921:1923	nov	1921:1923	nov	1921:1923	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	13	46	theme	1.2559T=KCTC	1944:1955	arg1	49678T					1957:1962	zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T	1927:1962	zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T	1927:1962	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	13	46	theme	1.2559T=KCTC	1944:1955	arg1	nov					1921:1923	nov	1921:1923	nov	1921:1923	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	8	47	theme	phosphatidylinositol	1108:1127	arg1	lipids					1161:1166	the major polar lipids	1145:1166	the major polar lipids detected in the three novel species	1145:1202	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	47	theme	phosphatidylinositol	1108:1127	arg1	mannosides					1129:1138	phosphatidylinositol mannosides	1108:1138	phosphatidylinositol mannosides	1108:1138	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	47	theme	phosphatidylinositol	1108:1127	arg1	Diphosphatidylglycerol					1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	47	theme	phosphatidylinositol	1108:1127	arg1	phosphatidylglycerol					1083:1102	phosphatidylglycerol	1083:1102	phosphatidylglycerol	1083:1102	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	6	48	theme	mol	816:818	arg1	%					819:819	73.0-74.6 mol%	806:819	73.0-74.6 mol%	806:819	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	4	49	theme	polyphasic	388:397	arg1	approach					399:406	a polyphasic approach	386:406	a polyphasic approach	386:406	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	11	50	theme	species	1505:1511	arg1	A4β					1517:1519	A4β	1517:1519	A4β	1517:1519	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	50	theme	species	1505:1511	arg1	type					1481:1484	The cell-wall peptidoglycan type	1453:1484	The cell-wall peptidoglycan type of the three novel species	1453:1511	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	51	theme	inferred	1530:1537	arg1	bridge					1564:1569	an inferred l-Orn-d-Asp interpeptide bridge	1527:1569	an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T	1527:1646	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	8	52	theme	major	1149:1153	arg1	lipids					1161:1166	the major polar lipids	1145:1166	the major polar lipids detected in the three novel species	1145:1202	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	52	theme	major	1149:1153	arg1	phosphatidylglycerol					1083:1102	phosphatidylglycerol	1083:1102	phosphatidylglycerol	1083:1102	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	52	theme	major	1149:1153	arg1	Diphosphatidylglycerol					1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	52	theme	major	1149:1153	arg1	mannosides					1129:1138	phosphatidylinositol mannosides	1108:1138	phosphatidylinositol mannosides	1108:1138	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	1	53	theme	wangleii	50:57	arg1	sp					59:60	Cellulomonas wangleii sp	37:60	Cellulomonas wangleii sp	37:60	nov., Cellulomonas wangleii sp.
36264676	1	53	theme	wangleii	50:57	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., Cellulomonas wangleii sp.
36264676	12	54	theme	phenotypic	1677:1686	arg1	hybridization					1711:1723	the phenotypic, phylogenetic, genomic hybridization	1673:1723	the phenotypic, phylogenetic, genomic hybridization	1673:1723	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	11	55	theme	interpeptide	1551:1562	arg1	bridge					1564:1569	an inferred l-Orn-d-Asp interpeptide bridge	1527:1569	an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T	1527:1646	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	56	theme	peptidoglycan	1467:1479	arg1	A4β					1517:1519	A4β	1517:1519	A4β	1517:1519	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	56	theme	peptidoglycan	1467:1479	arg1	type					1481:1484	The cell-wall peptidoglycan type	1453:1484	The cell-wall peptidoglycan type of the three novel species	1453:1511	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	11	57	with	A4β	1517:1519	arg1	bridge					1564:1569	an inferred l-Orn-d-Asp interpeptide bridge	1527:1569	an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T	1527:1646	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	5	58	located	observed	447:454	arg1	28					470:471	28	470:471	28	470:471	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	5	58	located	observed	447:454	arg1	pH					459:460	pH 7.0 and 28	459:471	pH	459:460	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	5	58	located	observed	447:454	arg2	growth					417:422	Optimal growth	409:422	Optimal growth of the six strains	409:441	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	5	59	theme	16S	512:514	arg1	gene					521:524	the 16S rRNA gene	508:524	the 16S rRNA gene	508:524	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	9	60	from	quinone	1234:1240	arg1	strain					1350:1355	strain zg-ZUI157T	1350:1366	strain zg-ZUI157T	1350:1366	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	10	61	located	detected	1390:1397	arg2	sugars					1383:1388	The cell-wall sugars	1369:1388	The cell-wall sugars detected in the three novel species	1369:1424	The cell-wall sugars detected in the three novel species mainly contained rhamnose.
36264676	10	61	located	detected	1390:1397	arg1	species					1418:1424	the three novel species	1402:1424	the three novel species	1402:1424	The cell-wall sugars detected in the three novel species mainly contained rhamnose.
36264676	12	62	theme	chemotaxonomic	1758:1771	arg1	analyses					1773:1780	chemotaxonomic analyses	1758:1780	chemotaxonomic analyses	1758:1780	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	15	63	theme	zg-ZUI188T=GDMCC	2073:2088	arg1	49674T					2103:2108	zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T	2073:2108	zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T	2073:2108	nov. (zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T) are proposed.
36264676	15	63	theme	zg-ZUI188T=GDMCC	2073:2088	arg1	nov					2067:2069	nov	2067:2069	nov	2067:2069	nov. (zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T) are proposed.
36264676	4	64	theme	Gram-stain-positive	170:188	arg1	bacteria					285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria	166:292	bacteria	285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	4	64	theme	Gram-stain-positive	170:188	arg1	zg-ZUI168					353:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	14	65	theme	fengjieae	2053:2061	arg1	sp					2063:2064	Cellulomonas fengjieae sp	2040:2064	Cellulomonas fengjieae sp	2040:2064	nov. (zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T) and Cellulomonas fengjieae sp.
36264676	6	66	theme	genus	755:759	arg1	Cellulomonas					761:772	the genus Cellulomonas	751:772	the genus Cellulomonas	751:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	4	67	theme	aerobic	191:197	arg1	bacteria					285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria	166:292	bacteria	285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	4	67	theme	aerobic	191:197	arg1	zg-ZUI168					353:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	7	68	theme	strain	1025:1030	arg1	pair					1032:1035	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	12	69	dep	phenotypic	1677:1686	arg1	genomic					1703:1709	genomic	1703:1709	genomic	1703:1709	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	12	69	dep	phenotypic	1677:1686	arg1	phylogenetic					1689:1700	phylogenetic	1689:1700	phylogenetic	1689:1700	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	6	70	theme	available	730:738	arg1	genomes					740:746	all available genomes	726:746	all available genomes in the genus Cellulomonas	726:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	5	71	theme	247	530:532	arg1	genes					539:543	247 core genes	530:543	247 core genes	530:543	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	12	72	theme	dongxiuzhuiae	1903:1915	arg1	sp					1917:1918	Cellulomonas dongxiuzhuiae sp	1890:1918	Cellulomonas dongxiuzhuiae sp	1890:1918	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	7	73	from	anteiso-C15:0	927:939	arg1	pair					1032:1035	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	6	74	theme	DNA-DNA	634:640	arg1	hybridization					642:654	digital DNA-DNA hybridization	626:654	digital DNA-DNA hybridization (19.3-30.1%)	626:667	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	74	theme	DNA-DNA	634:640	arg1	%					666:666	19.3-30.1%	657:666	19.3-30.1%	657:666	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	12	75	theme	novel	1842:1846	arg1	species					1861:1867	three novel Cellulomonas species	1836:1867	three novel Cellulomonas species	1836:1867	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	10	76	contain	contained	1433:1441	arg2	rhamnose					1443:1450	rhamnose	1443:1450	rhamnose	1443:1450	The cell-wall sugars detected in the three novel species mainly contained rhamnose.
36264676	10	76	contain	contained	1433:1441	arg1	sugars					1383:1388	The cell-wall sugars	1369:1388	The cell-wall sugars detected in the three novel species	1369:1424	The cell-wall sugars detected in the three novel species mainly contained rhamnose.
36264676	4	77	theme	facultative	202:212	arg1	bacteria					285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria	166:292	bacteria	285:292	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	4	77	theme	facultative	202:212	arg1	zg-ZUI168					353:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168	295:361	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	7	78	located	detected	844:851	arg1	pairs					863:867	strain pairs zg-ZUI157T/zg-ZUI40	856:887	strain pairs zg-ZUI157T/zg-ZUI40	856:887	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	7	78	located	detected	844:851	arg2	C16:0					920:924	C16:0	920:924	C16:0	920:924	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	7	78	located	detected	844:851	arg1	222T/zg-ZUI199					900:913	zg-ZUI 222T/zg-ZUI199	893:913	zg-ZUI 222T/zg-ZUI199	893:913	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	7	78	located	detected	844:851	arg2	acids					838:842	The major fatty acids	822:842	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199	822:913	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	6	79	theme	low	622:624	arg1	values					701:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values	622:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas	622:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	79	theme	low	622:624	arg1	%					718:718	78.0-85.5%	709:718	78.0-85.5%	709:718	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	7	80	theme	anteiso	945:951	arg1	A-C15:1					953:959	anteiso A-C15:1	945:959	anteiso A-C15:1	945:959	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	5	81	theme	Optimal	409:415	arg1	growth					417:422	Optimal growth	409:422	Optimal growth of the six strains	409:441	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	3	82	theme	Marmota	146:152	arg1	himalayana					154:163	Marmota himalayana	146:163	Marmota himalayana	146:163	nov., isolated from the intestinal contents of Marmota himalayana.
36264676	13	83	theme	Cellulomonas	1966:1977	arg1	sp					1988:1989	Cellulomonas wangleii sp	1966:1989	Cellulomonas wangleii sp	1966:1989	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	4	84	dep	aerobic	191:197	arg1	anaerobic					214:222	anaerobic	214:222	anaerobic	214:222	Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach.
36264676	8	85	located	detected	1168:1175	arg2	Diphosphatidylglycerol					1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	85	located	detected	1168:1175	arg1	species					1196:1202	the three novel species	1180:1202	the three novel species	1180:1202	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	85	located	detected	1168:1175	arg2	phosphatidylglycerol					1083:1102	phosphatidylglycerol	1083:1102	phosphatidylglycerol	1083:1102	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	85	located	detected	1168:1175	arg2	mannosides					1129:1138	phosphatidylinositol mannosides	1108:1138	phosphatidylinositol mannosides	1108:1138	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	85	located	detected	1168:1175	arg2	lipids					1161:1166	the major polar lipids	1145:1166	the major polar lipids detected in the three novel species	1145:1202	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	0	86	theme	dongxiuzhuiae	13:25	arg1	sp					27:28	Cellulomonas dongxiuzhuiae sp	0:28	Cellulomonas dongxiuzhuiae sp.	0:29	Cellulomonas dongxiuzhuiae sp.
36264676	5	87	theme	genus	574:578	arg1	Cellulomonas					580:591	genus Cellulomonas	574:591	genus Cellulomonas	574:591	Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas.
36264676	14	88	theme	zg-ZUI222T=GDMCC	1998:2013	arg1	49675T					2028:2033	zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T	1998:2033	zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T	1998:2033	nov. (zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T) and Cellulomonas fengjieae sp.
36264676	14	88	theme	zg-ZUI222T=GDMCC	1998:2013	arg1	nov					1992:1994	nov	1992:1994	nov	1992:1994	nov. (zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T) and Cellulomonas fengjieae sp.
36264676	6	89	theme	nucleotide	681:690	arg1	identity					692:699	average nucleotide identity	673:699	average nucleotide identity	673:699	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	13	90	theme	zg-ZUI157T=GDMCC	1927:1942	arg1	49678T					1957:1962	zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T	1927:1962	zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T	1927:1962	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	13	90	theme	zg-ZUI157T=GDMCC	1927:1942	arg1	nov					1921:1923	nov	1921:1923	nov	1921:1923	nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp.
36264676	12	91	theme	analyses	1773:1780	arg1	results					1662:1668	the results	1658:1668	the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses	1658:1780	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	6	92	theme	%	819:819	arg1	values					701:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values	622:706	low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas	622:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	92	theme	%	819:819	arg1	range					797:801	a DNA G+C content range	779:801	a DNA G+C content range of 73.0-74.6 mol%	779:819	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	6	92	theme	%	819:819	arg1	%					718:718	78.0-85.5%	709:718	78.0-85.5%	709:718	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	12	93	theme	average	1726:1732	arg1	identity					1745:1752	average nucleotide identity	1726:1752	average nucleotide identity	1726:1752	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	6	94	from	genomes	740:746	arg1	Cellulomonas					761:772	the genus Cellulomonas	751:772	the genus Cellulomonas	751:772	The three type strains have low digital DNA-DNA hybridization (19.3-30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas, and a DNA G+C content range of 73.0-74.6 mol%.
36264676	11	95	theme	novel	1499:1503	arg1	species					1505:1511	the three novel species	1489:1511	the three novel species	1489:1511	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	12	96	theme	identity	1745:1752	arg1	results					1662:1668	the results	1658:1668	the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses	1658:1780	Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp.
36264676	11	97	theme	strain	1630:1635	arg1	zg-ZUI188T					1637:1646	strain zg-ZUI188T	1630:1646	strain zg-ZUI188T	1630:1646	The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn-d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn-d-Glu for strain zg-ZUI188T.
36264676	7	98	theme	strain	856:861	arg1	pairs					863:867	strain pairs zg-ZUI157T/zg-ZUI40	856:887	strain pairs zg-ZUI157T/zg-ZUI40	856:887	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
36264676	8	99	theme	polar	1155:1159	arg1	lipids					1161:1166	the major polar lipids	1145:1166	the major polar lipids detected in the three novel species	1145:1202	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	99	theme	polar	1155:1159	arg1	phosphatidylglycerol					1083:1102	phosphatidylglycerol	1083:1102	phosphatidylglycerol	1083:1102	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	99	theme	polar	1155:1159	arg1	Diphosphatidylglycerol					1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol	1059:1080	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	8	99	theme	polar	1155:1159	arg1	mannosides					1129:1138	phosphatidylinositol mannosides	1108:1138	phosphatidylinositol mannosides	1108:1138	Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species.
36264676	1	100	theme	Cellulomonas	37:48	arg1	sp					59:60	Cellulomonas wangleii sp	37:60	Cellulomonas wangleii sp	37:60	nov., Cellulomonas wangleii sp.
36264676	1	100	theme	Cellulomonas	37:48	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., Cellulomonas wangleii sp.
36264676	9	101	located	detected	1242:1249	arg1	zg-ZUI222T					1262:1271	zg-ZUI222T	1262:1271	zg-ZUI222T	1262:1271	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	101	located	detected	1242:1249	arg1	zg-ZUI188T					1286:1295	zg-ZUI188T	1286:1295	zg-ZUI188T	1286:1295	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	101	located	detected	1242:1249	arg2	quinone					1234:1240	the predominant quinone	1218:1240	the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %)	1218:1304	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	101	located	detected	1242:1249	arg1	strains					1254:1260	strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %)	1254:1304	strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %)	1254:1304	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	9	101	located	detected	1242:1249	arg2	MK-9					1205:1208	MK-9	1205:1208	MK-9(H4)	1205:1212	MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T.
36264676	7	102	from	anteiso-C17:0	1008:1020	arg1	pair					1032:1035	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	strain pair zg-ZUI188T/zg-ZUI168	1025:1056	The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168.
35716917	4	0	theme	RAW264.7 cells	790:803	arg1	production					776:785	the nitric oxide (NO) production	754:785	the nitric oxide (NO) production of RAW264.7 cells induced by LPS	754:818	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	1	1	from	hepatitis	229:237	arg1	medicines					278:286	traditional Uygur medicines	260:286	traditional Uygur medicines	260:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	8	2	theme	significant	1546:1556	arg1	ratio					1569:1573	the most significant inhibition ratio	1537:1573	the most significant inhibition ratio against NO in RAW264.7 cells	1537:1602	RESULTS EOD showed the most significant inhibition ratio against NO in RAW264.7 cells without toxicity among the tested five fractions.
35716917	6	3	theme	semi-preparative	1181:1196	arg1	HPLC					1198:1201	semi-preparative HPLC	1181:1201	semi-preparative HPLC	1181:1201	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	2	4	theme	chemical	356:363	arg1	composition					365:375	chemical composition	356:375	chemical composition	356:375	AIM OF THE STUDY To investigate the anti-inflammatory activity and chemical composition of M. chamomilla, and clarify its molecular mechanism.
35716917	7	5	from	EOD	1453:1455	arg1	compounds					1438:1446	the identified compounds	1423:1446	the identified compounds from EOD	1423:1455	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	7	6	theme	Network	1396:1402	arg1	pharmacology					1404:1415	Network pharmacology	1396:1415	Network pharmacology using the identified compounds from EOD	1396:1455	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	7	7	theme	anti-inflammatory	1338:1354	arg1	mechanism					1356:1364	Then the underlying anti-inflammatory mechanism	1318:1364	Then the underlying anti-inflammatory mechanism of EOD	1318:1371	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	1	8	theme	Uygur	272:276	arg1	medicines					278:286	traditional Uygur medicines	260:286	traditional Uygur medicines	260:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	6	9	from	isolation	1142:1150	arg1	addition					1091:1098	addition	1091:1098	addition	1091:1098	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	7	10	theme	EOD	1369:1371	arg1	mechanism					1356:1364	Then the underlying anti-inflammatory mechanism	1318:1364	Then the underlying anti-inflammatory mechanism of EOD	1318:1371	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	11	11	theme	anti-inflammatory	2124:2140	arg1	targets					2142:2148	the most important anti-inflammatory targets	2105:2148	the most important anti-inflammatory targets of EOD predicted by Network pharmacology	2105:2189	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	11	11	theme	anti-inflammatory	2124:2140	arg1	IL-6					2060:2063	IL-6	2060:2063	IL-6	2060:2063	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	3	12	theme	water	701:705	arg1	EOW					717:719	EOW	717:719	EOW	717:719	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	12	theme	water	701:705	arg1	fraction					707:714	water fraction	701:714	water fraction (EOW)	701:720	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	12	theme	water	701:705	arg1	fractions					557:565	five fractions	552:565	five fractions	552:565	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	13	theme	petroleum	575:583	arg1	EOPE					601:604	EOPE	601:604	EOPE	601:604	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	13	theme	petroleum	575:583	arg1	fraction					591:598	petroleum ether fraction	575:598	petroleum ether fraction (EOPE)	575:605	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	13	theme	petroleum	575:583	arg1	fractions					557:565	five fractions	552:565	five fractions	552:565	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	1	14	theme	popular	189:195	arg1	tea					204:206	a popular herbal tea	187:206	a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines	187:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	1	14	theme	popular	189:195	arg1	Chamomile					147:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	116:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.)	116:182	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	11	15	theme	Network	2170:2176	arg1	pharmacology					2178:2189	Network pharmacology	2170:2189	Network pharmacology	2170:2189	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	6	16	theme	EOD	1133:1135	arg1	compounds					1120:1128	five most abundant compounds	1101:1128	five most abundant compounds of EOD	1101:1135	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	12	17	theme	CYP2E1	2484:2489	arg1	levels					2459:2464	the protein expression levels	2436:2464	the protein expression levels of Nrf2, HO-1 and CYP2E1	2436:2489	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	6	18	with	comparison	1281:1290	arg1	data					1297:1300	data	1297:1300	data in literatures	1297:1315	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	11	19	theme	important	2114:2122	arg1	targets					2142:2148	the most important anti-inflammatory targets	2105:2148	the most important anti-inflammatory targets of EOD predicted by Network pharmacology	2105:2189	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	11	19	theme	important	2114:2122	arg1	IL-6					2060:2063	IL-6	2060:2063	IL-6	2060:2063	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	compounds					1667:1675	Thirty-seven compounds	1654:1675	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene	1654:1814	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	triterpene					1805:1814	triterpene	1805:1814	triterpene	1805:1814	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	coumarin					1776:1783	coumarin	1776:1783	coumarin	1776:1783	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	flavonoid-O-glycoside					1687:1707	flavonoid-O-glycoside	1687:1707	flavonoid-O-glycoside	1687:1707	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	aglycone					1751:1758	methylated flavonoid aglycone	1730:1758	methylated flavonoid aglycone	1730:1758	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	sesquiterpene					1786:1798	sesquiterpene	1786:1798	sesquiterpene	1786:1798	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	aglycone					1720:1727	flavonoid aglycone	1710:1727	flavonoid aglycone	1710:1727	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	9	20	theme	Thirty-seven	1654:1665	arg1	acid					1770:1773	phenolic acid	1761:1773	phenolic acid	1761:1773	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	12	21	theme	protein	2440:2446	arg1	levels					2459:2464	the protein expression levels	2436:2464	the protein expression levels of Nrf2, HO-1 and CYP2E1	2436:2489	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	14	22	theme	anti-inflammatory	2664:2680	arg1	effect					2682:2687	anti-inflammatory effect	2664:2687	anti-inflammatory effect	2664:2687	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	1	23	theme	ETHNOPHARMACOLOGICAL	116:135	arg1	Matricaria					158:167	Matricaria	158:167	Matricaria	158:167	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	1	23	theme	ETHNOPHARMACOLOGICAL	116:135	arg1	Chamomile					147:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	116:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.)	116:182	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	1	23	theme	ETHNOPHARMACOLOGICAL	116:135	arg1	tea					204:206	a popular herbal tea	187:206	a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines	187:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	14	24	theme	M.	2642:2643	arg1	chamomilla					2645:2654	CONCLUSION M. chamomilla	2631:2654	CONCLUSION M. chamomilla	2631:2654	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	4	25	from	effect	744:749	arg1	production					776:785	the nitric oxide (NO) production	754:785	the nitric oxide (NO) production of RAW264.7 cells induced by LPS	754:818	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	12	26	theme	Western	2192:2198	arg1	Blot					2200:2203	Western Blot	2192:2203	Western Blot	2192:2203	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	5	27	theme	retention	1021:1029	arg1	time					1031:1034	retention time	1021:1034	retention time	1021:1034	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	6	28	with	analysis	1268:1275	arg1	data					1297:1300	data	1297:1300	data in literatures	1297:1315	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	6	29	from	data	1297:1300	arg1	literatures					1305:1315	literatures	1305:1315	literatures	1305:1315	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	13	30	theme	nuclear	2567:2573	arg1	translocation					2575:2587	the nuclear translocation	2563:2587	the nuclear translocation of Nrf2	2563:2595	In addition, EOD blocked the p65 protein into the nucleus and promoted the nuclear translocation of Nrf2 in RAW264.7 cells induced by LPS.
35716917	9	31	theme	methylated	1730:1739	arg1	aglycone					1751:1758	methylated flavonoid aglycone	1730:1758	methylated flavonoid aglycone	1730:1758	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	1	32	dep	Matricaria	158:167	arg1	L.					180:181	Matricaria chamomilla L.	158:181	Matricaria chamomilla L.	158:181	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	6	33	theme	data	1263:1266	arg1	analysis					1268:1275	HRMS and NMR data analysis	1250:1275	analysis	1268:1275	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	14	34	dep	pathways	2719:2726	arg1	pathways					2719:2726	NF-κB, MAPK and Nrf2/HO-1 pathways	2693:2726	pathways	2719:2726	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	14	34	dep	pathways	2719:2726	arg1	Nrf2/HO-1					2709:2717	Nrf2/HO-1	2709:2717	Nrf2/HO-1	2709:2717	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	14	34	dep	pathways	2719:2726	arg1	MAPK					2700:2703	MAPK	2700:2703	MAPK	2700:2703	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	0	35	theme	lipopolysaccharide	89:106	arg1	insult					108:113	lipopolysaccharide insult	89:113	lipopolysaccharide insult	89:113	Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult.
35716917	15	36	theme	natural	2796:2802	arg1	source					2804:2809	natural source	2796:2809	natural source	2796:2809	It could be further applied as a safe anti-inflammatory agent from natural source.
35716917	9	37	theme	phenolic	1761:1768	arg1	acid					1770:1773	phenolic acid	1761:1773	phenolic acid	1761:1773	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	0	38	theme	Chemical	0:7	arg1	component					9:17	Chemical component	0:17	Chemical component	0:17	Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult.
35716917	15	39	theme	safe	2762:2765	arg1	agent					2785:2789	a safe anti-inflammatory agent	2760:2789	a safe anti-inflammatory agent from natural source	2760:2809	It could be further applied as a safe anti-inflammatory agent from natural source.
35716917	15	39	theme	safe	2762:2765	arg1	It					2729:2730	It	2729:2730	It	2729:2730	It could be further applied as a safe anti-inflammatory agent from natural source.
35716917	2	40	theme	molecular	411:419	arg1	mechanism					421:429	its molecular mechanism	407:429	its molecular mechanism	407:429	AIM OF THE STUDY To investigate the anti-inflammatory activity and chemical composition of M. chamomilla, and clarify its molecular mechanism.
35716917	5	41	theme	absorption	1043:1052	arg1	comparison					1007:1016	comparison	1007:1016	comparison of retention time and UV absorption with authentic compounds	1007:1077	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	4	42	theme	further	904:910	arg1	investigations					912:925	further investigations	904:925	further investigations	904:925	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	4	43	theme	oxide	765:769	arg1	production					776:785	the nitric oxide (NO) production	754:785	the nitric oxide (NO) production of RAW264.7 cells induced by LPS	754:818	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	5	44	theme	authentic	1059:1067	arg1	compounds					1069:1077	authentic compounds	1059:1077	authentic compounds	1059:1077	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	3	45	dep	M.	454:455	arg1	chamomilla					457:466	chamomilla	457:466	chamomilla	457:466	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	12	46	theme	factors	2315:2321	arg1	levels					2292:2297	the protein expression levels	2269:2297	the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK),	2269:2420	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	3	47	theme	75	487:488	arg1	%					489:489	%	489:489	%	489:489	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	12	48	dep	Blot	2200:2203	arg1	experiments					2215:2225	experiments	2215:2225	experiments	2215:2225	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	8	49	theme	RESULTS	1518:1524	arg1	EOD					1526:1528	RESULTS EOD	1518:1528	RESULTS EOD	1518:1528	RESULTS EOD showed the most significant inhibition ratio against NO in RAW264.7 cells without toxicity among the tested five fractions.
35716917	3	50	theme	acetate	646:652	arg1	EOEA					664:667	EOEA	664:667	EOEA	664:667	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	50	theme	acetate	646:652	arg1	fraction					654:661	ethyl acetate fraction	640:661	ethyl acetate fraction (EOEA)	640:668	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	50	theme	acetate	646:652	arg1	fractions					557:565	five fractions	552:565	five fractions	552:565	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	12	51	dep	MAPKs	2390:2394	arg1	p-p38					2397:2401	p-p38	2397:2401	p-p38	2397:2401	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	12	51	dep	MAPKs	2390:2394	arg1	p-JNK					2414:2418	p-JNK	2414:2418	p-JNK	2414:2418	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	12	51	dep	MAPKs	2390:2394	arg1	p-ERK					2404:2408	p-ERK	2404:2408	p-ERK	2404:2408	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	1	52	theme	hepatitis	229:237	arg1	treatment					216:224	the treatment	212:224	the treatment of hepatitis and cholecystitis in traditional Uygur medicines	212:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	12	53	theme	expression	2281:2290	arg1	levels					2292:2297	the protein expression levels	2269:2297	the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK),	2269:2420	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	3	54	theme	n-butanol	671:679	arg1	EOB					691:693	EOB	691:693	EOB	691:693	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	54	theme	n-butanol	671:679	arg1	fractions					557:565	five fractions	552:565	five fractions	552:565	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	54	theme	n-butanol	671:679	arg1	fraction					681:688	n-butanol fraction	671:688	n-butanol fraction (EOB)	671:694	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	1	55	theme	cholecystitis	243:255	arg1	treatment					216:224	the treatment	212:224	the treatment of hepatitis and cholecystitis in traditional Uygur medicines	212:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	12	56	dep	factors	2315:2321	arg1	TNF-α					2343:2347	TNF-α	2343:2347	TNF-α	2343:2347	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	12	56	dep	factors	2315:2321	arg1	IL-6					2337:2340	IL-6	2337:2340	IL-6	2337:2340	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	12	56	dep	factors	2315:2321	arg1	MCP-1					2330:2334	MCP-1	2330:2334	MCP-1	2330:2334	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	12	56	dep	factors	2315:2321	arg1	PGE2					2324:2327	PGE2	2324:2327	PGE2	2324:2327	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	15	57	from	source	2804:2809	arg1	agent					2785:2789	a safe anti-inflammatory agent	2760:2789	a safe anti-inflammatory agent from natural source	2760:2809	It could be further applied as a safe anti-inflammatory agent from natural source.
35716917	15	57	from	source	2804:2809	arg1	It					2729:2730	It	2729:2730	It	2729:2730	It could be further applied as a safe anti-inflammatory agent from natural source.
35716917	1	58	theme	traditional	260:270	arg1	medicines					278:286	traditional Uygur medicines	260:286	traditional Uygur medicines	260:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	14	59	theme	CONCLUSION	2631:2640	arg1	chamomilla					2645:2654	CONCLUSION M. chamomilla	2631:2654	CONCLUSION M. chamomilla	2631:2654	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	8	60	theme	inhibition	1558:1567	arg1	ratio					1569:1573	the most significant inhibition ratio	1537:1573	the most significant inhibition ratio against NO in RAW264.7 cells	1537:1602	RESULTS EOD showed the most significant inhibition ratio against NO in RAW264.7 cells without toxicity among the tested five fractions.
35716917	6	61	theme	column	1155:1160	arg1	chromatography					1162:1175	column chromatography	1155:1175	column chromatography	1155:1175	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	8	62	theme	tested	1631:1636	arg1	fractions					1643:1651	five fractions	1638:1651	the tested five fractions	1627:1651	RESULTS EOD showed the most significant inhibition ratio against NO in RAW264.7 cells without toxicity among the tested five fractions.
35716917	7	63	theme	underlying	1327:1336	arg1	mechanism					1356:1364	Then the underlying anti-inflammatory mechanism	1318:1364	Then the underlying anti-inflammatory mechanism of EOD	1318:1371	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	9	64	theme	authentic	1873:1881	arg1	compounds					1883:1891	authentic compounds	1873:1891	authentic compounds	1873:1891	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	1	65	from	medicines	278:286	arg1	treatment					216:224	the treatment	212:224	the treatment of hepatitis and cholecystitis in traditional Uygur medicines	212:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	6	66	theme	HRMS	1250:1253	arg1	analysis					1268:1275	HRMS and NMR data analysis	1250:1275	analysis	1268:1275	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	2	67	theme	STUDY	300:304	arg1	AIM					289:291	AIM	289:291	AIM OF THE STUDY	289:304	AIM OF THE STUDY To investigate the anti-inflammatory activity and chemical composition of M. chamomilla, and clarify its molecular mechanism.
35716917	1	68	theme	herbal	197:202	arg1	tea					204:206	a popular herbal tea	187:206	a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines	187:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	1	68	theme	herbal	197:202	arg1	Chamomile					147:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	116:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.)	116:182	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	4	69	theme	fractions	832:840	arg1	Cytotoxicity					723:734	Cytotoxicity	723:734	Cytotoxicity	723:734	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	4	69	theme	fractions	832:840	arg1	effect					744:749	the effect	740:749	the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions	740:840	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	11	70	theme	EOD	2153:2155	arg1	targets					2142:2148	the most important anti-inflammatory targets	2105:2148	the most important anti-inflammatory targets of EOD predicted by Network pharmacology	2105:2189	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	11	70	theme	EOD	2153:2155	arg1	IL-6					2060:2063	IL-6	2060:2063	IL-6	2060:2063	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	10	71	theme	abundant	1908:1915	arg1	axillarin					1969:1977	axillarin (26)	1969:1982	axillarin (26)	1969:1982	The five most abundant compounds in EOD were isolated and determined to be axillarin (26), tricin (30), chrysoeriol (31), centaureidin (33) and chrysosplenetin (35).
35716917	10	71	theme	abundant	1908:1915	arg1	compounds					1917:1925	The five most abundant compounds	1894:1925	The five most abundant compounds in EOD	1894:1932	The five most abundant compounds in EOD were isolated and determined to be axillarin (26), tricin (30), chrysoeriol (31), centaureidin (33) and chrysosplenetin (35).
35716917	1	72	from	cholecystitis	243:255	arg1	medicines					278:286	traditional Uygur medicines	260:286	traditional Uygur medicines	260:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	5	73	theme	LC-MS/MS	969:976	arg1	analysis					978:985	LC-MS/MS analysis	969:985	LC-MS/MS analysis	969:985	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	12	74	theme	expression	2448:2457	arg1	levels					2459:2464	the protein expression levels	2436:2464	the protein expression levels of Nrf2, HO-1 and CYP2E1	2436:2489	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	8	75	from	ratio	1569:1573	arg1	RAW264.7 cells					1589:1602	RAW264.7 cells	1589:1602	RAW264.7 cells	1589:1602	RESULTS EOD showed the most significant inhibition ratio against NO in RAW264.7 cells without toxicity among the tested five fractions.
35716917	3	76	theme	ether	585:589	arg1	EOPE					601:604	EOPE	601:604	EOPE	601:604	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	76	theme	ether	585:589	arg1	fraction					591:598	petroleum ether fraction	575:598	petroleum ether fraction (EOPE)	575:605	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	76	theme	ether	585:589	arg1	fractions					557:565	five fractions	552:565	five fractions	552:565	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	12	77	theme	Nrf2	2469:2472	arg1	levels					2459:2464	the protein expression levels	2436:2464	the protein expression levels of Nrf2, HO-1 and CYP2E1	2436:2489	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	6	78	theme	abundant	1111:1118	arg1	compounds					1120:1128	five most abundant compounds	1101:1128	five most abundant compounds of EOD	1101:1135	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	5	79	with	comparison	1007:1016	arg1	compounds					1069:1077	authentic compounds	1059:1077	authentic compounds	1059:1077	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	1	80	from	treatment	216:224	arg1	medicines					278:286	traditional Uygur medicines	260:286	traditional Uygur medicines	260:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	5	81	theme	EOD	946:948	arg1	components					932:941	The components	928:941	The components of EOD	928:948	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	3	82	theme	dichloromethane	608:622	arg1	EOD					634:636	EOD	634:636	EOD	634:636	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	82	theme	dichloromethane	608:622	arg1	fractions					557:565	five fractions	552:565	five fractions	552:565	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	82	theme	dichloromethane	608:622	arg1	fraction					624:631	dichloromethane fraction	608:631	dichloromethane fraction (EOD)	608:637	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	1	83	theme	RELEVANCE	137:145	arg1	Matricaria					158:167	Matricaria	158:167	Matricaria	158:167	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	1	83	theme	RELEVANCE	137:145	arg1	Chamomile					147:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile	116:155	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.)	116:182	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	1	83	theme	RELEVANCE	137:145	arg1	tea					204:206	a popular herbal tea	187:206	a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines	187:286	ETHNOPHARMACOLOGICAL RELEVANCE Chamomile (Matricaria chamomilla L.) is a popular herbal tea for the treatment of hepatitis and cholecystitis in traditional Uygur medicines.
35716917	12	84	theme	HO-1	2475:2478	arg1	levels					2459:2464	the protein expression levels	2436:2464	the protein expression levels of Nrf2, HO-1 and CYP2E1	2436:2489	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	5	85	with	combination	990:1000	arg1	comparison					1007:1016	comparison	1007:1016	comparison of retention time and UV absorption with authentic compounds	1007:1077	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	2	86	theme	anti-inflammatory	325:341	arg1	activity					343:350	anti-inflammatory activity	325:350	anti-inflammatory activity	325:350	AIM OF THE STUDY To investigate the anti-inflammatory activity and chemical composition of M. chamomilla, and clarify its molecular mechanism.
35716917	0	87	theme	chamomilla	65:74	arg1	effects					43:49	in vitro protective effects	23:49	in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult	23:113	Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult.
35716917	0	87	theme	chamomilla	65:74	arg1	component					9:17	Chemical component	0:17	Chemical component	0:17	Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult.
35716917	7	88	dep	Blot	1490:1493	arg1	experiments					1505:1515	experiments	1505:1515	experiments	1505:1515	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	9	89	theme	flavonoid	1710:1718	arg1	aglycone					1720:1727	flavonoid aglycone	1710:1727	flavonoid aglycone	1710:1727	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	5	90	theme	time	1031:1034	arg1	comparison					1007:1016	comparison	1007:1016	comparison of retention time and UV absorption with authentic compounds	1007:1077	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	10	91	from	compounds	1917:1925	arg1	EOD					1930:1932	EOD	1930:1932	EOD	1930:1932	The five most abundant compounds in EOD were isolated and determined to be axillarin (26), tricin (30), chrysoeriol (31), centaureidin (33) and chrysosplenetin (35).
35716917	4	92	from	Cytotoxicity	723:734	arg1	production					776:785	the nitric oxide (NO) production	754:785	the nitric oxide (NO) production of RAW264.7 cells induced by LPS	754:818	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	7	93	theme	Western	1482:1488	arg1	Blot					1490:1493	Western Blot	1482:1493	Western Blot	1482:1493	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	11	94	theme	ERK2	2082:2085	arg1	cascade					2087:2093	ERK1 and ERK2 cascade	2073:2093	ERK1 and ERK2 cascade	2073:2093	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	11	94	theme	ERK2	2082:2085	arg1	IL-6					2060:2063	IL-6	2060:2063	IL-6	2060:2063	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	3	95	theme	ethyl	640:644	arg1	EOEA					664:667	EOEA	664:667	EOEA	664:667	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	95	theme	ethyl	640:644	arg1	fraction					654:661	ethyl acetate fraction	640:661	ethyl acetate fraction (EOEA)	640:668	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	3	95	theme	ethyl	640:644	arg1	fractions					557:565	five fractions	552:565	five fractions	552:565	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	5	96	theme	UV	1040:1041	arg1	absorption					1043:1052	UV absorption	1040:1052	UV absorption	1040:1052	The components of EOD were identified by LC-MS/MS analysis in combination with comparison of retention time and UV absorption with authentic compounds by HPLC.
35716917	9	97	theme	flavonoid	1741:1749	arg1	aglycone					1751:1758	methylated flavonoid aglycone	1730:1758	methylated flavonoid aglycone	1730:1758	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	6	98	theme	NMR	1259:1261	arg1	analysis					1268:1275	HRMS and NMR data analysis	1250:1275	analysis	1268:1275	In addition, five most abundant compounds of EOD were isolation by column chromatography and semi-preparative HPLC and their structures were further confirmed by HRMS and NMR data analysis and comparison with data in literatures.
35716917	13	99	theme	Nrf2	2592:2595	arg1	translocation					2575:2587	the nuclear translocation	2563:2587	the nuclear translocation of Nrf2	2563:2595	In addition, EOD blocked the p65 protein into the nucleus and promoted the nuclear translocation of Nrf2 in RAW264.7 cells induced by LPS.
35716917	3	100	theme	M.	454:455	arg1	MATERIALS					432:440	MATERIALS	432:440	MATERIALS	432:440	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	15	101	theme	anti-inflammatory	2767:2783	arg1	agent					2785:2789	a safe anti-inflammatory agent	2760:2789	a safe anti-inflammatory agent from natural source	2760:2809	It could be further applied as a safe anti-inflammatory agent from natural source.
35716917	15	101	theme	anti-inflammatory	2767:2783	arg1	It					2729:2730	It	2729:2730	It	2729:2730	It could be further applied as a safe anti-inflammatory agent from natural source.
35716917	14	102	theme	NF-κB	2693:2697	arg1	pathways					2719:2726	NF-κB, MAPK and Nrf2/HO-1 pathways	2693:2726	pathways	2719:2726	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	14	102	theme	NF-κB	2693:2697	arg1	Nrf2/HO-1					2709:2717	Nrf2/HO-1	2709:2717	Nrf2/HO-1	2709:2717	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	14	102	theme	NF-κB	2693:2697	arg1	MAPK					2700:2703	MAPK	2700:2703	MAPK	2700:2703	CONCLUSION M. chamomilla exerted anti-inflammatory effect via NF-κB, MAPK and Nrf2/HO-1 pathways.
35716917	2	103	theme	chamomilla	383:392	arg1	activity					343:350	anti-inflammatory activity	325:350	anti-inflammatory activity	325:350	AIM OF THE STUDY To investigate the anti-inflammatory activity and chemical composition of M. chamomilla, and clarify its molecular mechanism.
35716917	2	103	theme	chamomilla	383:392	arg1	composition					365:375	chemical composition	356:375	chemical composition	356:375	AIM OF THE STUDY To investigate the anti-inflammatory activity and chemical composition of M. chamomilla, and clarify its molecular mechanism.
35716917	0	104	theme	in	23:24	arg1	effects					43:49	in vitro protective effects	23:49	in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult	23:113	Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult.
35716917	2	105	dep	activity	343:350	arg1	the					321:323	the	321:323	the	321:323	AIM OF THE STUDY To investigate the anti-inflammatory activity and chemical composition of M. chamomilla, and clarify its molecular mechanism.
35716917	0	106	theme	protective	32:41	arg1	effects					43:49	in vitro protective effects	23:49	in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult	23:113	Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult.
35716917	11	107	theme	ERK1	2073:2076	arg1	cascade					2087:2093	ERK1 and ERK2 cascade	2073:2093	ERK1 and ERK2 cascade	2073:2093	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	11	107	theme	ERK1	2073:2076	arg1	IL-6					2060:2063	IL-6	2060:2063	IL-6	2060:2063	IL-6, NF-κB, ERK1 and ERK2 cascade, TNF were the most important anti-inflammatory targets of EOD predicted by Network pharmacology.
35716917	12	108	theme	inflammatory	2302:2313	arg1	factors					2315:2321	inflammatory factors	2302:2321	inflammatory factors (PGE2, MCP-1, IL-6, TNF-α)	2302:2348	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	12	108	theme	inflammatory	2302:2313	arg1	iNOS					2351:2354	iNOS	2351:2354	iNOS	2351:2354	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	4	109	theme	nitric	758:763	arg1	NO					772:773	NO	772:773	NO	772:773	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	4	109	theme	nitric	758:763	arg1	oxide					765:769	nitric oxide	758:769	the nitric oxide (NO) production of RAW264.7 cells induced by LPS	754:818	Cytotoxicity and the effect on the nitric oxide (NO) production of RAW264.7 cells induced by LPS of the five fractions were screened, and the most active one (EOD) was selected for further investigations.
35716917	13	110	theme	p65	2521:2523	arg1	protein					2525:2531	the p65 protein	2517:2531	the p65 protein	2517:2531	In addition, EOD blocked the p65 protein into the nucleus and promoted the nuclear translocation of Nrf2 in RAW264.7 cells induced by LPS.
35716917	0	111	dep	in	23:24	arg1	vitro					26:30	vitro	26:30	vitro	26:30	Chemical component and in vitro protective effects of Matricaria chamomilla (L.) against lipopolysaccharide insult.
35716917	12	112	theme	protein	2273:2279	arg1	levels					2292:2297	the protein expression levels	2269:2297	the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK),	2269:2420	Western Blot and ELISA experiments revealed that EOD significantly decreased the protein expression levels of inflammatory factors (PGE2, MCP-1, IL-6, TNF-α), iNOS, COX-2, NF-κB (p-P65 and p-IκBα), MAPKs (p-p38, p-ERK and p-JNK), and increased the protein expression levels of Nrf2, HO-1 and CYP2E1.
35716917	9	113	with	comparison	1857:1866	arg1	compounds					1883:1891	authentic compounds	1873:1891	authentic compounds	1873:1891	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	3	114	theme	%	489:489	arg1	ethanol					491:497	75% ethanol	487:497	75% ethanol	487:497	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
35716917	7	115	theme	identified	1427:1436	arg1	compounds					1438:1446	the identified compounds	1423:1446	the identified compounds from EOD	1423:1455	Then the underlying anti-inflammatory mechanism of EOD were predicted through Network pharmacology using the identified compounds from EOD, and further verified by Western Blot and ELISA experiments.
35716917	9	116	with	LC-MS/MS	1844:1851	arg1	compounds					1883:1891	authentic compounds	1873:1891	authentic compounds	1873:1891	Thirty-seven compounds including flavonoid-O-glycoside, flavonoid aglycone, methylated flavonoid aglycone, phenolic acid, coumarin, sesquiterpene, and triterpene were identified from EOD by LC-MS/MS and comparison with authentic compounds.
35716917	3	117	theme	different	523:531	arg1	solvents					533:540	different solvents	523:540	different solvents	523:540	MATERIALS AND METHODS M. chamomilla was extracted with 75% ethanol and then extracted with different solvents to obtain five fractions, namely petroleum ether fraction (EOPE), dichloromethane fraction (EOD), ethyl acetate fraction (EOEA), n-butanol fraction (EOB), and water fraction (EOW).
36710626	10	0	theme	starch	1984:1989	arg1	biosynthesis					1991:2002	starch biosynthesis	1984:2002	starch biosynthesis in upcoming day	1984:2018	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	0	1	from	Arabidopsis	156:166	arg1	inhibition					89:98	inhibition	89:98	inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis	89:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	2	2	theme	proteins	362:369	arg1	synthesis					343:351	the synthesis	339:351	the synthesis of plant proteins, carbohydrates, and lipids	339:396	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	1	3	theme	abiotic/biotic	260:273	arg1	stresses					275:282	abiotic/biotic stresses	260:282	abiotic/biotic stresses	260:282	As sessile organism, plants daily encounter dynamic and challenging environments including abiotic/biotic stresses.
36710626	10	4	theme	starch	1970:1975	arg1	overconsumption					1951:1965	overconsumption	1951:1965	overconsumption of starch	1951:1975	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	7	5	theme	expression	1407:1416	arg1	homologs					1342:1349	its close homologs	1332:1349	its close homologs (PIF1, PIF3 and PIF5)	1332:1371	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	5	theme	expression	1407:1416	arg1	regulators					1389:1398	negative regulators	1380:1398	negative regulators of QQS expression	1380:1416	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	5	theme	expression	1407:1416	arg1	PIF4					1323:1326	PIF4	1323:1326	PIF4	1323:1326	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	3	6	dep	starch	593:598	arg1	i.e.					588:591	i.e.	588:591	i.e.	588:591	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	0	7	theme	degradation	62:72	arg1	adjustment					74:83	starch degradation adjustment	55:83	starch degradation adjustment	55:83	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	4	8	theme	carbohydrate	665:676	arg1	production					678:687	carbohydrate production	665:687	carbohydrate production	665:687	However, it is not fully understood how light signaling affect carbohydrate production and allocation in plant growth and development.
36710626	10	9	theme	PIF	1852:1854	arg1	factors					1872:1878	PIF light signaling factors	1852:1878	PIF light signaling factors	1852:1878	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	9	10	theme	QQS	1818:1820	arg1	expression					1804:1813	expression	1804:1813	expression of QQS	1804:1820	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	0	11	theme	regulator	120:128	arg1	STARCH					140:145	carbon metabolic regulator QUA-QUINE STARCH	103:145	carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis	103:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	0	11	theme	regulator	120:128	arg1	QQS					148:150	QQS	148:150	QQS	148:150	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	3	12	theme	resource	565:572	arg1	partitioning					574:585	resource partitioning	565:585	resource partitioning (i.e. starch)	565:599	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	3	12	theme	resource	565:572	arg1	starch					593:598	starch	593:598	starch	593:598	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	7	13	theme	PIF4	1301:1304	arg1	overexpression					1283:1296	overexpression	1283:1296	overexpression of PIF4	1283:1304	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	9	14	dep	H3	1685:1686	arg1	methyltransferases					1691:1708	K4 methyltransferases	1688:1708	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7)	1677:1738	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	5	15	theme	metabolic	831:839	arg1	processes					841:849	metabolic processes	831:849	metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates	831:922	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	9	16	theme	methylation	1646:1656	arg1	machinery					1658:1666	DNA methylation machinery	1642:1666	DNA methylation machinery	1642:1666	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	7	17	theme	close	1336:1340	arg1	regulators					1389:1398	negative regulators	1380:1398	negative regulators of QQS expression	1380:1416	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	17	theme	close	1336:1340	arg1	PIF5					1367:1370	PIF5	1367:1370	PIF5	1367:1370	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	17	theme	close	1336:1340	arg1	homologs					1342:1349	its close homologs	1332:1349	its close homologs (PIF1, PIF3 and PIF5)	1332:1371	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	17	theme	close	1336:1340	arg1	PIF1					1352:1355	PIF1	1352:1355	PIF1	1352:1355	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	17	theme	close	1336:1340	arg1	PIF4					1323:1326	PIF4	1323:1326	PIF4	1323:1326	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	17	theme	close	1336:1340	arg1	PIF3					1358:1361	PIF3	1358:1361	PIF3	1358:1361	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	10	18	theme	signaling	1862:1870	arg1	factors					1872:1878	PIF light signaling factors	1852:1878	PIF light signaling factors	1852:1878	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	9	19	dep	ATX1	1717:1720	arg1	e.g.					1711:1714	e.g.	1711:1714	e.g.	1711:1714	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	6	20	theme	starch	1184:1189	arg1	reserves					1191:1198	starch reserves	1184:1198	starch reserves	1184:1198	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	3	21	theme	plant	544:548	arg1	metabolism					550:559	plant metabolism	544:559	plant metabolism	544:559	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	9	22	theme	histone	1677:1683	arg1	H3					1685:1686	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7)	1677:1738	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7)	1677:1738	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	5	23	from	composition	953:963	arg1	Arabidopsis					987:997	Arabidopsis	987:997	Arabidopsis	987:997	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	0	24	theme	starch	55:60	arg1	adjustment					74:83	starch degradation adjustment	55:83	starch degradation adjustment	55:83	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	9	25	theme	K4	1688:1689	arg1	methyltransferases					1691:1708	K4 methyltransferases	1688:1708	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7)	1677:1738	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	2	26	dep	carbon	303:308	arg1	allocations					323:333	allocations	323:333	allocations	323:333	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	10	27	theme	starch	1919:1924	arg1	starch					1919:1924	starch	1919:1924	starch	1919:1924	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	10	27	theme	starch	1919:1924	arg1	amounts					1908:1914	optimal amounts	1900:1914	optimal amounts of starch	1900:1924	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	8	28	theme	evening	1449:1455	arg1	complex					1457:1463	the evening complex	1445:1463	the evening complex including ELF3	1445:1478	In addition, we show that the evening complex including ELF3 is required for active expression of QQS, thus playing a positive role in starch catabolism during night period.
36710626	0	29	theme	carbon	103:108	arg1	STARCH					140:145	carbon metabolic regulator QUA-QUINE STARCH	103:145	carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis	103:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	0	29	theme	carbon	103:108	arg1	QQS					148:150	QQS	148:150	QQS	148:150	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	6	30	theme	TRANSCRIPTION	1057:1069	arg1	PIFs					1080:1083	PIFs	1080:1083	PIFs	1080:1083	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	6	30	theme	TRANSCRIPTION	1057:1069	arg1	PIF4					1096:1099	PIF4	1096:1099	PIF4	1096:1099	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	6	30	theme	TRANSCRIPTION	1057:1069	arg1	FACTORS					1071:1077	PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS	1028:1077	PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4	1028:1099	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	7	31	theme	QQS	1201:1203	arg1	expression					1205:1214	QQS expression	1201:1214	QQS expression	1201:1214	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	2	32	theme	lipids	391:396	arg1	synthesis					343:351	the synthesis	339:351	the synthesis of plant proteins, carbohydrates, and lipids	339:396	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	0	33	theme	PHYTOCHROME-INTERACTING	0:22	arg1	PIFs					33:36	PIFs	33:36	PIFs	33:36	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	0	33	theme	PHYTOCHROME-INTERACTING	0:22	arg1	FACTORS					24:30	PHYTOCHROME-INTERACTING FACTORS	0:30	PHYTOCHROME-INTERACTING FACTORS (PIFs)	0:37	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	0	34	from	inhibition	89:98	arg1	Arabidopsis					156:166	Arabidopsis	156:166	Arabidopsis	156:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	9	35	dep	HAC1	1773:1776	arg1	e.g.					1767:1770	e.g.	1767:1770	e.g.	1767:1770	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	6	36	theme	PHYTOCHROME-INTERACTING	1028:1050	arg1	PIFs					1080:1083	PIFs	1080:1083	PIFs	1080:1083	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	6	36	theme	PHYTOCHROME-INTERACTING	1028:1050	arg1	PIF4					1096:1099	PIF4	1096:1099	PIF4	1096:1099	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	6	36	theme	PHYTOCHROME-INTERACTING	1028:1050	arg1	FACTORS					1071:1077	PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS	1028:1077	PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4	1028:1099	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	10	37	from	biosynthesis	1991:2002	arg1	day					2016:2018	upcoming day	2007:2018	upcoming day	2007:2018	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	2	38	theme	plant	417:421	arg1	growth					423:428	plant growth	417:428	plant growth	417:428	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	3	39	theme	essential	482:490	arg1	signals					506:512	the essential environmental signals	478:512	the essential environmental signals	478:512	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	5	40	theme	QUA-QUINE	793:801	arg1	QQS					811:813	QQS	811:813	QQS	811:813	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	5	40	theme	QUA-QUINE	793:801	arg1	STARCH					803:808	QUA-QUINE STARCH	793:808	QUA-QUINE STARCH (QQS)	793:814	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	8	41	theme	QQS	1517:1519	arg1	expression					1503:1512	active expression	1496:1512	active expression of QQS	1496:1519	In addition, we show that the evening complex including ELF3 is required for active expression of QQS, thus playing a positive role in starch catabolism during night period.
36710626	1	42	theme	dynamic	213:219	arg1	environments					237:248	dynamic and challenging environments	213:248	dynamic and challenging environments including abiotic/biotic stresses	213:282	As sessile organism, plants daily encounter dynamic and challenging environments including abiotic/biotic stresses.
36710626	1	42	theme	dynamic	213:219	arg1	stresses					275:282	abiotic/biotic stresses	260:282	abiotic/biotic stresses	260:282	As sessile organism, plants daily encounter dynamic and challenging environments including abiotic/biotic stresses.
36710626	0	43	from	STARCH	140:145	arg1	Arabidopsis					156:166	Arabidopsis	156:166	Arabidopsis	156:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	7	44	dep	homologs	1342:1349	arg1	homologs					1342:1349	its close homologs	1332:1349	its close homologs (PIF1, PIF3 and PIF5)	1332:1371	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	44	dep	homologs	1342:1349	arg1	PIF5					1367:1370	PIF5	1367:1370	PIF5	1367:1370	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	44	dep	homologs	1342:1349	arg1	PIF3					1358:1361	PIF3	1358:1361	PIF3	1358:1361	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	44	dep	homologs	1342:1349	arg1	PIF1					1352:1355	PIF1	1352:1355	PIF1	1352:1355	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	1	45	theme	challenging	225:235	arg1	environments					237:248	dynamic and challenging environments	213:248	dynamic and challenging environments including abiotic/biotic stresses	213:282	As sessile organism, plants daily encounter dynamic and challenging environments including abiotic/biotic stresses.
36710626	1	45	theme	challenging	225:235	arg1	stresses					275:282	abiotic/biotic stresses	260:282	abiotic/biotic stresses	260:282	As sessile organism, plants daily encounter dynamic and challenging environments including abiotic/biotic stresses.
36710626	3	46	theme	signals	506:512	arg1	Light					464:468	Light	464:468	Light	464:468	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	3	46	theme	signals	506:512	arg1	signals					506:512	the essential environmental signals	478:512	the essential environmental signals	478:512	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	3	46	theme	signals	506:512	arg1	one					471:473	one	471:473	one	471:473	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	4	47	from	allocation	693:702	arg1	growth					713:718	plant growth	707:718	plant growth	707:718	However, it is not fully understood how light signaling affect carbohydrate production and allocation in plant growth and development.
36710626	4	47	from	allocation	693:702	arg1	development					724:734	development	724:734	development	724:734	However, it is not fully understood how light signaling affect carbohydrate production and allocation in plant growth and development.
36710626	2	48	theme	carbon	303:308	arg1	fundamental					401:411	fundamental	401:411	fundamental	401:411	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	2	48	theme	carbon	303:308	arg1	regulation					289:298	The regulation	285:298	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids	285:396	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	4	49	from	production	678:687	arg1	growth					713:718	plant growth	707:718	plant growth	707:718	However, it is not fully understood how light signaling affect carbohydrate production and allocation in plant growth and development.
36710626	4	49	from	production	678:687	arg1	development					724:734	development	724:734	development	724:734	However, it is not fully understood how light signaling affect carbohydrate production and allocation in plant growth and development.
36710626	7	50	theme	QQS	1403:1405	arg1	expression					1407:1416	QQS expression	1403:1416	QQS expression	1403:1416	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	51	from	de-repressed	1233:1244	arg1	pifQ					1258:1261	pifQ	1258:1261	pifQ	1258:1261	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	51	from	de-repressed	1233:1244	arg1	pif4					1249:1252	pif4	1249:1252	pif4	1249:1252	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	52	theme	negative	1380:1387	arg1	homologs					1342:1349	its close homologs	1332:1349	its close homologs (PIF1, PIF3 and PIF5)	1332:1371	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	52	theme	negative	1380:1387	arg1	regulators					1389:1398	negative regulators	1380:1398	negative regulators of QQS expression	1380:1416	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	7	52	theme	negative	1380:1387	arg1	PIF4					1323:1326	PIF4	1323:1326	PIF4	1323:1326	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	8	53	theme	starch	1554:1559	arg1	catabolism					1561:1570	starch catabolism	1554:1570	starch catabolism during night period	1554:1590	In addition, we show that the evening complex including ELF3 is required for active expression of QQS, thus playing a positive role in starch catabolism during night period.
36710626	2	54	theme	plant	356:360	arg1	proteins					362:369	plant proteins	356:369	plant proteins	356:369	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	9	55	theme	H3	1744:1745	arg1	acetyltransferases					1747:1764	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases	1677:1764	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5)	1677:1786	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	7	56	from	pif4	1249:1252	arg1	de-repressed					1233:1244	de-repressed	1233:1244	de-repressed	1233:1244	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	6	57	theme	dawn	1136:1139	arg1	period					1141:1146	the dawn period	1132:1146	the dawn period	1132:1146	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	10	58	theme	upcoming	2007:2014	arg1	day					2016:2018	upcoming day	2007:2018	upcoming day	2007:2018	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	4	59	theme	light	642:646	arg1	signaling					648:656	light signaling	642:656	light signaling	642:656	However, it is not fully understood how light signaling affect carbohydrate production and allocation in plant growth and development.
36710626	5	60	from	defense	976:982	arg1	Arabidopsis					987:997	Arabidopsis	987:997	Arabidopsis	987:997	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	1	61	theme	sessile	172:178	arg1	organism					180:187	sessile organism	172:187	sessile organism	172:187	As sessile organism, plants daily encounter dynamic and challenging environments including abiotic/biotic stresses.
36710626	7	62	from	pifQ	1258:1261	arg1	de-repressed					1233:1244	de-repressed	1233:1244	de-repressed	1233:1244	QQS expression is significantly de-repressed in pif4 and pifQ, while repressed by overexpression of PIF4, suggesting that PIF4 and its close homologs (PIF1, PIF3 and PIF5) act as negative regulators of QQS expression.
36710626	0	63	theme	QUA-QUINE	130:138	arg1	STARCH					140:145	carbon metabolic regulator QUA-QUINE STARCH	103:145	carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis	103:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	0	63	theme	QUA-QUINE	130:138	arg1	QQS					148:150	QQS	148:150	QQS	148:150	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	2	64	theme	nitrogen	314:321	arg1	fundamental					401:411	fundamental	401:411	fundamental	401:411	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	2	64	theme	nitrogen	314:321	arg1	regulation					289:298	The regulation	285:298	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids	285:396	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	5	65	from	leaf	942:945	arg1	Arabidopsis					987:997	Arabidopsis	987:997	Arabidopsis	987:997	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	9	66	theme	DNA	1642:1644	arg1	machinery					1658:1666	DNA methylation machinery	1642:1666	DNA methylation machinery	1642:1666	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	6	67	theme	reserves	1191:1198	arg1	overconsumption					1165:1179	overconsumption	1165:1179	overconsumption of starch reserves	1165:1198	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	4	68	theme	plant	707:711	arg1	growth					713:718	plant growth	707:718	plant growth	707:718	However, it is not fully understood how light signaling affect carbohydrate production and allocation in plant growth and development.
36710626	10	69	theme	light	1856:1860	arg1	factors					1872:1878	PIF light signaling factors	1852:1878	PIF light signaling factors	1852:1878	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	10	70	theme	optimal	1900:1906	arg1	starch					1919:1924	starch	1919:1924	starch	1919:1924	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	10	70	theme	optimal	1900:1906	arg1	amounts					1908:1914	optimal amounts	1900:1914	optimal amounts of starch	1900:1924	This study demonstrates that PIF light signaling factors help plants utilize optimal amounts of starch during night and prevent overconsumption of starch before starch biosynthesis in upcoming day.
36710626	5	71	theme	unique	752:757	arg1	gene					747:750	An orphan gene	737:750	An orphan gene	737:750	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	5	72	theme	carbon	871:876	arg1	partitioning					855:866	partitioning	855:866	partitioning of carbon and nitrogen among proteins and carbohydrates	855:922	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	9	73	theme	H3	1685:1686	arg1	acetyltransferases					1747:1764	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases	1677:1764	histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5)	1677:1786	Furthermore, QQS is epigenetically suppressed by DNA methylation machinery, whereas histone H3 K4 methyltransferases (e.g., ATX1, ATX2, and ATXR7) and H3 acetyltransferases (e.g., HAC1 and HAC5) are involved in expression of QQS.
36710626	5	74	theme	Arabidopsis	762:772	arg1	thaliana					774:781	Arabidopsis thaliana	762:781	Arabidopsis thaliana	762:781	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	5	75	theme	nitrogen	882:889	arg1	partitioning					855:866	partitioning	855:866	partitioning of carbon and nitrogen among proteins and carbohydrates	855:922	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	5	76	theme	seed	948:951	arg1	composition					953:963	seed composition	948:963	seed composition	948:963	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	0	77	theme	metabolic	110:118	arg1	STARCH					140:145	carbon metabolic regulator QUA-QUINE STARCH	103:145	carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis	103:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	0	77	theme	metabolic	110:118	arg1	QQS					148:150	QQS	148:150	QQS	148:150	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	5	78	theme	orphan	740:745	arg1	gene					747:750	An orphan gene	737:750	An orphan gene	737:750	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	6	79	theme	bHLH	1052:1055	arg1	PIFs					1080:1083	PIFs	1080:1083	PIFs	1080:1083	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	6	79	theme	bHLH	1052:1055	arg1	PIF4					1096:1099	PIF4	1096:1099	PIF4	1096:1099	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	6	79	theme	bHLH	1052:1055	arg1	FACTORS					1071:1077	PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS	1028:1077	PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4	1028:1099	In this study, we show that PHYTOCHROME-INTERACTING bHLH TRANSCRIPTION FACTORS (PIFs) including PIF4 is required to suppress QQS at the dawn period, thus preventing overconsumption of starch reserves.
36710626	8	80	theme	active	1496:1501	arg1	expression					1503:1512	active expression	1496:1512	active expression of QQS	1496:1519	In addition, we show that the evening complex including ELF3 is required for active expression of QQS, thus playing a positive role in starch catabolism during night period.
36710626	0	81	theme	STARCH	140:145	arg1	inhibition					89:98	inhibition	89:98	inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis	89:166	PHYTOCHROME-INTERACTING FACTORS (PIFs) are involved in starch degradation adjustment via inhibition of carbon metabolic regulator QUA-QUINE STARCH (QQS) in Arabidopsis.
36710626	3	82	theme	environmental	492:504	arg1	signals					506:512	the essential environmental signals	478:512	the essential environmental signals	478:512	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36710626	8	83	theme	night	1579:1583	arg1	period					1585:1590	night period	1579:1590	night period	1579:1590	In addition, we show that the evening complex including ELF3 is required for active expression of QQS, thus playing a positive role in starch catabolism during night period.
36710626	5	84	theme	plant	970:974	arg1	defense					976:982	plant defense	970:982	plant defense	970:982	An orphan gene unique to Arabidopsis thaliana, named as QUA-QUINE STARCH (QQS) is involved in metabolic processes for partitioning of carbon and nitrogen among proteins and carbohydrates, thus influencing leaf, seed composition, and plant defense in Arabidopsis.
36710626	2	85	theme	carbohydrates	372:384	arg1	synthesis					343:351	the synthesis	339:351	the synthesis of plant proteins, carbohydrates, and lipids	339:396	The regulation of carbon and nitrogen allocations for the synthesis of plant proteins, carbohydrates, and lipids is fundamental for plant growth and adaption to its surroundings.
36710626	8	86	theme	positive	1537:1544	arg1	role					1546:1549	a positive role	1535:1549	a positive role	1535:1549	In addition, we show that the evening complex including ELF3 is required for active expression of QQS, thus playing a positive role in starch catabolism during night period.
36710626	3	87	theme	substantial	522:532	arg1	impact					534:539	substantial impact	522:539	substantial impact	522:539	Light, one of the essential environmental signals, exerts substantial impact on plant metabolism and resource partitioning (i.e. starch).
36917734	0	0	theme	-Induced	67:74	arg1	Colitis					82:88	Dextran Sulfate Sodium (DSS)-Induced Acute Colitis	39:88	Dextran Sulfate Sodium (DSS)-Induced Acute Colitis	39:88	Alanyl-Glutamine (Ala-Gln) Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Colitis by Regulating the Gut Microbiota, PI3K-Akt/NF-κB/STAT3 Signaling, and Associated Pulmonary Injury.
36917734	1	1	theme	pulmonary	314:322	arg1	injury					324:329	pulmonary injury	314:329	pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice	314:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	0	2	theme	Pulmonary	171:179	arg1	Injury					181:186	Associated Pulmonary Injury	160:186	Associated Pulmonary Injury	160:186	Alanyl-Glutamine (Ala-Gln) Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Colitis by Regulating the Gut Microbiota, PI3K-Akt/NF-κB/STAT3 Signaling, and Associated Pulmonary Injury.
36917734	5	3	theme	acute	1273:1277	arg1	colitis					1279:1285	DSS-induced acute colitis	1261:1285	DSS-induced acute colitis	1261:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	5	4	theme	CD4+T-cell	1209:1218	arg1	subsets					1220:1226	CD4+T-cell subsets	1209:1226	CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis	1209:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	5	5	theme	pulmonary	1154:1162	arg1	injury					1164:1169	the pulmonary injury	1150:1169	the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis	1150:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	0	6	theme	Associated	160:169	arg1	Injury					181:186	Associated Pulmonary Injury	160:186	Associated Pulmonary Injury	160:186	Alanyl-Glutamine (Ala-Gln) Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Colitis by Regulating the Gut Microbiota, PI3K-Akt/NF-κB/STAT3 Signaling, and Associated Pulmonary Injury.
36917734	4	7	theme	DSS-induced	1077:1087	arg1	colitis					1095:1101	DSS-induced acute colitis	1077:1101	DSS-induced acute colitis	1077:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	1	8	theme	protective	234:243	arg1	effect					245:250	the protective effect	230:250	the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice	230:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	4	9	theme	Ala-Gln	934:940	arg1	treatment					942:950	Ala-Gln treatment	934:950	Ala-Gln treatment	934:950	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	6	10	theme	accompanying	1452:1463	arg1	injury					1475:1480	accompanying pulmonary injury	1452:1480	accompanying pulmonary injury	1452:1480	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	3	11	with	mice	724:727	arg1	colitis					753:759	DSS- induced acute colitis	734:759	DSS- induced acute colitis	734:759	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	6	12	theme	pulmonary	1465:1473	arg1	injury					1475:1480	accompanying pulmonary injury	1452:1480	accompanying pulmonary injury	1452:1480	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	0	13	theme	Acute	76:80	arg1	Colitis					82:88	Dextran Sulfate Sodium (DSS)-Induced Acute Colitis	39:88	Dextran Sulfate Sodium (DSS)-Induced Acute Colitis	39:88	Alanyl-Glutamine (Ala-Gln) Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Colitis by Regulating the Gut Microbiota, PI3K-Akt/NF-κB/STAT3 Signaling, and Associated Pulmonary Injury.
36917734	2	14	theme	lymph	603:607	arg1	nodes					609:613	mesenteric lymph nodes	592:613	mesenteric lymph nodes (MLNs)	592:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	14	theme	lymph	603:607	arg1	MLNs					616:619	MLNs	616:619	MLNs	616:619	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	3	15	theme	Clostridia-vadinBB60	887:906	arg1	abundances					852:861	the abundances	848:861	the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes	848:921	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	5	16	theme	pulmonary	1231:1239	arg1	tissue					1241:1246	pulmonary tissue	1231:1246	pulmonary tissue in mice with DSS-induced acute colitis	1231:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	1	17	theme	dextran	342:348	arg1	DSS					366:368	DSS	366:368	DSS	366:368	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	1	17	theme	dextran	342:348	arg1	sodium					358:363	dextran sulfate sodium	342:363	dextran sulfate sodium (DSS) in C57BL/6 mice	342:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	5	18	from	levels	1199:1204	arg1	tissue					1241:1246	pulmonary tissue	1231:1246	pulmonary tissue in mice with DSS-induced acute colitis	1231:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	2	19	theme	mesenteric	592:601	arg1	nodes					609:613	mesenteric lymph nodes	592:613	mesenteric lymph nodes (MLNs)	592:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	19	theme	mesenteric	592:601	arg1	MLNs					616:619	MLNs	616:619	MLNs	616:619	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	6	20	theme	acute	1334:1338	arg1	colitis					1340:1346	DSS-induced acute colitis	1322:1346	DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury	1322:1480	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	1	21	theme	sulfate	350:356	arg1	DSS					366:368	DSS	366:368	DSS	366:368	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	1	21	theme	sulfate	350:356	arg1	sodium					358:363	dextran sulfate sodium	342:363	dextran sulfate sodium (DSS) in C57BL/6 mice	342:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	2	22	theme	subsets	581:587	arg1	number					560:565	the absolute number	547:565	the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs)	547:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	4	23	theme	pathways	1042:1049	arg1	activation					980:989	the activation	976:989	the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis	976:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	3	24	theme	Gastranaerophilales	866:884	arg1	abundances					852:861	the abundances	848:861	the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes	848:921	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	2	25	theme	Ala-Gln	412:418	arg1	intervention					420:431	Ala-Gln intervention	412:431	Ala-Gln intervention	412:431	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	26	theme	CD4+T-cell	570:579	arg1	subsets					581:587	CD4+T-cell subsets	570:587	CD4+T-cell subsets in mesenteric lymph nodes (MLNs)	570:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	3	27	theme	gut	706:708	arg1	microbiota					710:719	the gut microbiota	702:719	the gut microbiota	702:719	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	0	28	theme	PI3K-Akt/NF-κB/STAT3	124:143	arg1	Signaling					145:153	PI3K-Akt/NF-κB/STAT3 Signaling	124:153	PI3K-Akt/NF-κB/STAT3 Signaling	124:153	Alanyl-Glutamine (Ala-Gln) Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Colitis by Regulating the Gut Microbiota, PI3K-Akt/NF-κB/STAT3 Signaling, and Associated Pulmonary Injury.
36917734	1	29	from	sodium	358:363	arg1	mice					382:385	C57BL/6 mice	374:385	C57BL/6 mice	374:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	1	30	theme	alanyl-glutamine	255:270	arg1	effect					245:250	the protective effect	230:250	the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice	230:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	3	31	dep	ameliorated	671:681	arg1	decreasing					776:785	decreasing	776:785	decreasing the relative abundance of Desulfovibrionaceae	776:831	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	3	31	dep	ameliorated	671:681	arg1	increasing					837:846	increasing	837:846	increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes	837:921	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	2	32	theme	absolute	551:558	arg1	number					560:565	the absolute number	547:565	the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs)	547:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	6	33	dep	colitis	1340:1346	arg1	alleviating					1440:1450	alleviating	1440:1450	alleviating accompanying pulmonary injury	1440:1480	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	6	33	dep	colitis	1340:1346	arg1	regulating					1351:1360	regulating	1351:1360	regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways	1351:1423	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	3	34	theme	microbiota	710:719	arg1	composition					687:697	the composition	683:697	the composition of the gut microbiota in mice with DSS- induced acute colitis	683:759	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	0	35	theme	Gut	108:110	arg1	Microbiota					112:121	the Gut Microbiota	104:121	the Gut Microbiota	104:121	Alanyl-Glutamine (Ala-Gln) Ameliorates Dextran Sulfate Sodium (DSS)-Induced Acute Colitis by Regulating the Gut Microbiota, PI3K-Akt/NF-κB/STAT3 Signaling, and Associated Pulmonary Injury.
36917734	3	36	from	composition	687:697	arg1	mice					724:727	mice	724:727	mice with DSS- induced acute colitis	724:759	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	2	37	from	number	560:565	arg1	nodes					609:613	mesenteric lymph nodes	592:613	mesenteric lymph nodes (MLNs)	592:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	37	from	number	560:565	arg1	MLNs					616:619	MLNs	616:619	MLNs	616:619	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	5	38	from	injury	1164:1169	arg1	levels					1199:1204	levels	1199:1204	levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis	1199:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	6	39	theme	DSS-induced	1322:1332	arg1	colitis					1340:1346	DSS-induced acute colitis	1322:1346	DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury	1322:1480	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	4	40	with	mice	1067:1070	arg1	colitis					1095:1101	DSS-induced acute colitis	1077:1101	DSS-induced acute colitis	1077:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	4	41	theme	PI3K-Akt/NF-κB/STAT3	998:1017	arg1	pathways					1042:1049	the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways	994:1049	the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis	994:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	4	42	from	pathways	1042:1049	arg1	colon					1058:1062	the colon	1054:1062	the colon of mice with DSS-induced acute colitis	1054:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	2	43	theme	colon	491:495	arg1	shortening					497:506	colon shortening	491:506	colon shortening	491:506	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	3	44	theme	Alistipes	913:921	arg1	abundances					852:861	the abundances	848:861	the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes	848:921	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	5	45	theme	subsets	1220:1226	arg1	levels					1199:1204	levels	1199:1204	levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis	1199:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	2	46	theme	weight	444:449	arg1	loss					451:454	weight loss	444:454	weight loss	444:454	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	3	47	theme	relative	791:798	arg1	abundance					800:808	the relative abundance	787:808	the relative abundance of Desulfovibrionaceae	787:831	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	6	48	theme	gut	1366:1368	arg1	microflora					1370:1379	the gut microflora	1362:1379	the gut microflora	1362:1379	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	4	49	theme	signaling	1032:1040	arg1	pathways					1042:1049	the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways	994:1049	the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis	994:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	1	50	theme	acute	285:289	arg1	colitis					291:297	acute colitis	285:297	acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice	285:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	6	51	theme	signaling	1406:1414	arg1	pathways					1416:1423	PI3K-Akt/NF-κB/STAT3 signaling pathways	1385:1423	PI3K-Akt/NF-κB/STAT3 signaling pathways	1385:1423	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	5	52	from	tissue	1241:1246	arg1	mice					1251:1254	mice	1251:1254	mice with DSS-induced acute colitis	1251:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	5	52	from	tissue	1241:1246	arg1	levels					1199:1204	levels	1199:1204	levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis	1199:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	4	53	theme	inflammatory	1019:1030	arg1	pathways					1042:1049	the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways	994:1049	the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis	994:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	1	54	from	effect	245:250	arg1	colitis					291:297	acute colitis	285:297	acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice	285:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	1	55	theme	C57BL/6	374:380	arg1	mice					382:385	C57BL/6 mice	374:385	C57BL/6 mice	374:385	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	5	56	from	subsets	1220:1226	arg1	tissue					1241:1246	pulmonary tissue	1231:1246	pulmonary tissue in mice with DSS-induced acute colitis	1231:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	3	57	theme	Desulfovibrionaceae	813:831	arg1	abundance					800:808	the relative abundance	787:808	the relative abundance of Desulfovibrionaceae	787:831	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	2	58	from	subsets	581:587	arg1	nodes					609:613	mesenteric lymph nodes	592:613	mesenteric lymph nodes (MLNs)	592:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	58	from	subsets	581:587	arg1	MLNs					616:619	MLNs	616:619	MLNs	616:619	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	59	theme	pathological	513:524	arg1	injury					526:531	pathological injury	513:531	pathological injury	513:531	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	5	60	theme	Ala-Gln	1113:1119	arg1	intervention					1121:1132	Ala-Gln intervention	1113:1132	Ala-Gln intervention	1113:1132	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	5	61	from	imbalance	1186:1194	arg1	levels					1199:1204	levels	1199:1204	levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis	1199:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	4	62	theme	acute	1089:1093	arg1	colitis					1095:1101	DSS-induced acute colitis	1077:1101	DSS-induced acute colitis	1077:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	1	63	theme	study	205:209	arg1	aim					193:195	The aim	189:195	The aim of this study	189:209	The aim of this study was to investigate the protective effect of alanyl-glutamine (Ala-Gln) on acute colitis complicated by pulmonary injury induced by dextran sulfate sodium (DSS) in C57BL/6 mice.
36917734	5	64	with	mice	1251:1254	arg1	colitis					1279:1285	DSS-induced acute colitis	1261:1285	DSS-induced acute colitis	1261:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	2	65	from	nodes	609:613	arg1	number					560:565	the absolute number	547:565	the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs)	547:620	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	6	66	theme	PI3K-Akt/NF-κB/STAT3	1385:1404	arg1	pathways					1416:1423	PI3K-Akt/NF-κB/STAT3 signaling pathways	1385:1423	PI3K-Akt/NF-κB/STAT3 signaling pathways	1385:1423	In conclusion, Ala-Gln alleviates DSS-induced acute colitis by regulating the gut microflora and PI3K-Akt/NF-κB/STAT3 signaling pathways, as well as by alleviating accompanying pulmonary injury.
36917734	4	67	from	activation	980:989	arg1	colon					1058:1062	the colon	1054:1062	the colon of mice with DSS-induced acute colitis	1054:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	2	68	theme	activity	469:476	arg1	DAI					485:487	DAI	485:487	DAI	485:487	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	68	theme	activity	469:476	arg1	index					478:482	the disease activity index	457:482	the disease activity index (DAI)	457:488	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	5	69	theme	DSS-induced	1261:1271	arg1	colitis					1279:1285	DSS-induced acute colitis	1261:1285	DSS-induced acute colitis	1261:1285	Notably, Ala-Gln intervention also alleviated the pulmonary injury as well as the imbalance in levels of CD4+T-cell subsets in pulmonary tissue in mice with DSS-induced acute colitis.
36917734	4	70	from	colon	1058:1062	arg1	activation					980:989	the activation	976:989	the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis	976:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	3	71	theme	induced	739:745	arg1	colitis					753:759	DSS- induced acute colitis	734:759	DSS- induced acute colitis	734:759	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	2	72	theme	disease	461:467	arg1	DAI					485:487	DAI	485:487	DAI	485:487	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	2	72	theme	disease	461:467	arg1	index					478:482	the disease activity index	457:482	the disease activity index (DAI)	457:488	The results showed that Ala-Gln intervention alleviated weight loss, the disease activity index (DAI), colon shortening, and pathological injury and regulated the absolute number of CD4+T-cell subsets in mesenteric lymph nodes (MLNs).
36917734	3	73	theme	Ala-Gln	636:642	arg1	intervention					644:655	Ala-Gln intervention	636:655	Ala-Gln intervention	636:655	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	3	74	theme	acute	747:751	arg1	colitis					753:759	DSS- induced acute colitis	734:759	DSS- induced acute colitis	734:759	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36917734	4	75	theme	mice	1067:1070	arg1	colon					1058:1062	the colon	1054:1062	the colon of mice with DSS-induced acute colitis	1054:1101	Moreover, Ala-Gln treatment significantly inhibited the activation of the PI3K-Akt/NF-κB/STAT3 inflammatory signaling pathways in the colon of mice with DSS-induced acute colitis.
36917734	3	76	theme	DSS-	734:737	arg1	colitis					753:759	DSS- induced acute colitis	734:759	DSS- induced acute colitis	734:759	In addition, Ala-Gln intervention significantly ameliorated the composition of the gut microbiota in mice with DSS- induced acute colitis, significantly decreasing the relative abundance of Desulfovibrionaceae and increasing the abundances of Gastranaerophilales, Clostridia-vadinBB60, and Alistipes.
36567940	8	0	theme	sialylation	1745:1755	arg1	remodeling					1723:1732	the remodeling	1719:1732	the remodeling of surface sialylation caused by increased sialidase activity	1719:1794	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	2	1	theme	multiple	382:389	arg1	taxa					401:404	multiple microbial taxa	382:404	multiple microbial taxa	382:404	Carbohydrate foraging requires the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa.
36567940	8	2	theme	observed	1820:1827	arg1	exacerbation					1829:1840	the observed exacerbation	1816:1840	the observed exacerbation of acute colitis in mice	1816:1865	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	8	3	with	Treatment	1539:1547	arg1	sialidase					1598:1606	bacterial sialidase	1588:1606	bacterial sialidase	1588:1606	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	7	4	theme	mucosal	1522:1528	arg1	glycans					1530:1536	mucosal glycans	1522:1536	mucosal glycans	1522:1536	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	3	5	theme	cell	586:589	arg1	adhesion					591:598	cell adhesion	586:598	cell adhesion	586:598	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	4	6	theme	specific	899:906	arg1	enzymes					947:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	7	7	theme	acid	1514:1517	arg1	distribution					1491:1502	the distribution	1487:1502	the distribution of sialic acid on mucosal glycans	1487:1536	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	4	8	theme	activities	702:711	arg1	impact					662:667	the impact	658:667	the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans	658:764	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	8	9	theme	colitis	1851:1857	arg1	exacerbation					1829:1840	the observed exacerbation	1816:1840	the observed exacerbation of acute colitis in mice	1816:1865	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	4	10	theme	dextran	987:993	arg1	sodium					1003:1008	dextran sulfate sodium	987:1008	dextran sulfate sodium ingestion	987:1018	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	5	11	theme	disease	1154:1160	arg1	severity					1162:1169	disease severity	1154:1169	disease severity	1154:1169	Whereas increased fucosidase and rhamnosidase activity did not alter the course of colitis, increased sialidase activity exacerbated disease severity.
36567940	1	12	theme	microbiota	212:221	arg1	composition					189:199	the composition	185:199	the composition of the gut microbiota	185:221	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract influences the composition of the gut microbiota.
36567940	5	13	theme	fucosidase	1039:1048	arg1	activity					1067:1074	increased fucosidase and rhamnosidase activity	1029:1074	increased fucosidase and rhamnosidase activity	1029:1074	Whereas increased fucosidase and rhamnosidase activity did not alter the course of colitis, increased sialidase activity exacerbated disease severity.
36567940	4	14	theme	sodium	1003:1008	arg1	ingestion					1010:1018	dextran sulfate sodium ingestion	987:1018	dextran sulfate sodium ingestion	987:1018	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	4	15	theme	glycoside	682:690	arg1	activities					702:711	bacterial glycoside hydrolase activities	672:711	bacterial glycoside hydrolase activities	672:711	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	4	16	theme	acute	962:966	arg1	colitis					968:974	acute colitis	962:974	acute colitis induced by dextran sulfate sodium ingestion	962:1018	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	0	17	from	colitis	70:76	arg1	mice					81:84	mice	81:84	mice	81:84	Increase of intestinal bacterial sialidase activity exacerbates acute colitis in mice.
36567940	7	18	theme	mice	1452:1455	arg1	colon					1435:1439	the colon	1431:1439	the colon of treated mice	1431:1455	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	4	19	theme	host	753:756	arg1	glycans					758:764	host glycans	753:764	host glycans	753:764	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	5	20	theme	increased	1113:1121	arg1	activity					1133:1140	increased sialidase activity	1113:1140	increased sialidase activity	1113:1140	Whereas increased fucosidase and rhamnosidase activity did not alter the course of colitis, increased sialidase activity exacerbated disease severity.
36567940	1	21	theme	dietary	122:128	arg1	carbohydrates					130:142	endogenous and dietary carbohydrates	107:142	endogenous and dietary carbohydrates	107:142	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract influences the composition of the gut microbiota.
36567940	8	22	theme	propria	1559:1565	arg1	cells					1577:1581	lamina propria dendritic cells	1552:1581	lamina propria dendritic cells	1552:1581	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	6	23	theme	similar	1299:1305	arg1	shift					1307:1311	a similar shift	1297:1311	a similar shift in gut bacteria	1297:1327	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	6	24	from	shift	1307:1311	arg1	bacteria					1320:1327	gut bacteria	1316:1327	gut bacteria	1316:1327	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	2	25	theme	glycoside	289:297	arg1	hydrolases					299:308	bacterially-encoded glycoside hydrolases	269:308	bacterially-encoded glycoside hydrolases	269:308	Carbohydrate foraging requires the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa.
36567940	8	26	theme	cells	1577:1581	arg1	Treatment					1539:1547	Treatment	1539:1547	Treatment of lamina propria dendritic cells with bacterial sialidase	1539:1606	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	4	27	theme	microbial	724:732	arg1	composition					734:744	the gut microbial composition	716:744	the gut microbial composition	716:744	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	3	28	from	processes	615:623	arg1	lumen					636:640	the gut lumen	628:640	the gut lumen	628:640	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	1	29	theme	gastrointestinal	151:166	arg1	tract					168:172	the gastrointestinal tract	147:172	the gastrointestinal tract	147:172	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract influences the composition of the gut microbiota.
36567940	8	30	theme	bacterial	1588:1596	arg1	sialidase					1598:1606	bacterial sialidase	1588:1606	bacterial sialidase	1588:1606	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	4	31	theme	local	806:810	arg1	hydrolase					822:830	local glycoside hydrolase	806:830	local glycoside hydrolase activity	806:839	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	1	32	theme	endogenous	107:116	arg1	carbohydrates					130:142	endogenous and dietary carbohydrates	107:142	endogenous and dietary carbohydrates	107:142	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract influences the composition of the gut microbiota.
36567940	4	33	theme	hydrolase	822:830	arg1	activity					832:839	local glycoside hydrolase activity	806:839	local glycoside hydrolase activity	806:839	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	7	34	theme	sialidase	1409:1417	arg1	activity					1419:1426	Increased sialidase activity	1399:1426	Increased sialidase activity in the colon of treated mice	1399:1455	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	8	35	theme	sialylated	1647:1656	arg1	ligands					1658:1664	sialylated ligands	1647:1664	sialylated ligands	1647:1664	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	0	36	theme	intestinal	12:21	arg1	activity					43:50	intestinal bacterial sialidase activity	12:50	intestinal bacterial sialidase activity	12:50	Increase of intestinal bacterial sialidase activity exacerbates acute colitis in mice.
36567940	5	37	theme	increased	1029:1037	arg1	activity					1067:1074	increased fucosidase and rhamnosidase activity	1029:1074	increased fucosidase and rhamnosidase activity	1029:1074	Whereas increased fucosidase and rhamnosidase activity did not alter the course of colitis, increased sialidase activity exacerbated disease severity.
36567940	8	38	from	colitis	1851:1857	arg1	mice					1862:1865	mice	1862:1865	mice	1862:1865	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	3	39	theme	host	477:480	arg1	glycans					482:488	host glycans	477:488	host glycans	477:488	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	0	40	theme	sialidase	33:41	arg1	activity					43:50	intestinal bacterial sialidase activity	12:50	intestinal bacterial sialidase activity	12:50	Increase of intestinal bacterial sialidase activity exacerbates acute colitis in mice.
36567940	8	41	theme	siglec	1687:1692	arg1	lectins					1694:1700	anti-inflammatory siglec lectins	1669:1700	anti-inflammatory siglec lectins	1669:1700	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	3	42	theme	glycoside	503:511	arg1	hydrolases					513:522	bacterial glycoside hydrolases	493:522	bacterial glycoside hydrolases	493:522	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	8	43	theme	increased	1767:1775	arg1	activity					1787:1794	increased sialidase activity	1767:1794	increased sialidase activity	1767:1794	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	6	44	theme	activity	1206:1213	arg1	effect					1176:1181	The effect	1172:1181	The effect of increased sialidase activity on inflammation	1172:1229	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	8	45	from	mice	1862:1865	arg1	exacerbation					1829:1840	the observed exacerbation	1816:1840	the observed exacerbation of acute colitis in mice	1816:1865	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	6	46	from	effect	1176:1181	arg1	inflammation					1218:1229	inflammation	1218:1229	inflammation	1218:1229	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	1	47	from	availability	91:102	arg1	tract					168:172	the gastrointestinal tract	147:172	the gastrointestinal tract	147:172	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract influences the composition of the gut microbiota.
36567940	7	48	from	activity	1419:1426	arg1	colon					1435:1439	the colon	1431:1439	the colon of treated mice	1431:1455	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	6	49	theme	increased	1186:1194	arg1	activity					1206:1213	increased sialidase activity	1186:1213	increased sialidase activity	1186:1213	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	2	50	theme	microbial	391:399	arg1	taxa					401:404	multiple microbial taxa	382:404	multiple microbial taxa	382:404	Carbohydrate foraging requires the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa.
36567940	4	51	theme	fucosidase	919:928	arg1	enzymes					947:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	8	52	theme	surface	1737:1743	arg1	sialylation					1745:1755	surface sialylation	1737:1755	surface sialylation caused by increased sialidase activity	1737:1794	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	6	53	theme	mice	1355:1358	arg1	mice					1355:1358	mice	1355:1358	mice supplemented with recombinant E. coli	1355:1396	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	6	53	theme	mice	1355:1358	arg1	groups					1345:1350	all groups	1341:1350	all groups of mice supplemented with recombinant E. coli	1341:1396	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	4	54	theme	rhamnosidase	934:945	arg1	enzymes					947:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	6	55	theme	microbial	1264:1272	arg1	composition					1274:1284	the microbial composition	1260:1284	the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli	1260:1396	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	3	56	theme	glycoproteins	560:572	arg1	properties					538:547	the properties	534:547	the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen	534:640	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	4	57	theme	hydrolase	692:700	arg1	activities					702:711	bacterial glycoside hydrolase activities	672:711	bacterial glycoside hydrolase activities	672:711	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	3	58	theme	activation	604:613	arg1	processes					615:623	cell adhesion and activation processes	586:623	cell adhesion and activation processes in the gut lumen	586:640	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	8	59	theme	acute	1845:1849	arg1	colitis					1851:1857	acute colitis	1845:1857	acute colitis in mice	1845:1865	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	4	60	theme	sialidase	908:916	arg1	enzymes					947:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	specific sialidase, fucosidase and rhamnosidase enzymes	899:953	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	7	61	theme	sialic	1507:1512	arg1	acid					1514:1517	sialic acid	1507:1517	sialic acid	1507:1517	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	3	62	theme	adhesion	591:598	arg1	processes					615:623	cell adhesion and activation processes	586:623	cell adhesion and activation processes in the gut lumen	586:640	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	1	63	theme	gut	208:210	arg1	microbiota					212:221	the gut microbiota	204:221	the gut microbiota	204:221	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract influences the composition of the gut microbiota.
36567940	3	64	theme	gut	632:634	arg1	lumen					636:640	the gut lumen	628:640	the gut lumen	628:640	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	4	65	from	impact	662:667	arg1	composition					734:744	the gut microbial composition	716:744	the gut microbial composition	716:744	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	4	65	from	impact	662:667	arg1	glycans					758:764	host glycans	753:764	host glycans	753:764	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	4	66	theme	sulfate	995:1001	arg1	sodium					1003:1008	dextran sulfate sodium	987:1008	dextran sulfate sodium ingestion	987:1018	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	8	67	theme	lamina	1552:1557	arg1	cells					1577:1581	lamina propria dendritic cells	1552:1581	lamina propria dendritic cells	1552:1581	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	6	68	theme	gut	1316:1318	arg1	bacteria					1320:1327	gut bacteria	1316:1327	gut bacteria	1316:1327	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	4	69	theme	bacterial	672:680	arg1	activities					702:711	bacterial glycoside hydrolase activities	672:711	bacterial glycoside hydrolase activities	672:711	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	8	70	theme	dendritic	1567:1575	arg1	cells					1577:1581	lamina propria dendritic cells	1552:1581	lamina propria dendritic cells	1552:1581	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	5	71	theme	sialidase	1123:1131	arg1	activity					1133:1140	increased sialidase activity	1113:1140	increased sialidase activity	1113:1140	Whereas increased fucosidase and rhamnosidase activity did not alter the course of colitis, increased sialidase activity exacerbated disease severity.
36567940	2	72	theme	hydrolases	299:308	arg1	action					259:264	the action	255:264	the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa	255:404	Carbohydrate foraging requires the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa.
36567940	1	73	theme	carbohydrates	130:142	arg1	availability					91:102	The availability	87:102	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract	87:172	The availability of endogenous and dietary carbohydrates in the gastrointestinal tract influences the composition of the gut microbiota.
36567940	7	74	from	distribution	1491:1502	arg1	glycans					1530:1536	mucosal glycans	1522:1536	mucosal glycans	1522:1536	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	7	75	theme	treated	1444:1450	arg1	mice					1452:1455	treated mice	1444:1455	treated mice	1444:1455	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	2	76	theme	carbon	364:369	arg1	sources					371:377	carbon sources	364:377	carbon sources	364:377	Carbohydrate foraging requires the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa.
36567940	2	77	theme	bacterially-encoded	269:287	arg1	hydrolases					299:308	bacterially-encoded glycoside hydrolases	269:308	bacterially-encoded glycoside hydrolases	269:308	Carbohydrate foraging requires the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa.
36567940	4	78	theme	gut	720:722	arg1	composition					734:744	the gut microbial composition	716:744	the gut microbial composition	716:744	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	3	79	gly	glycoproteins	560:572	arg1	glycoproteins					560:572	surface glycoproteins	552:572	surface glycoproteins involved in cell adhesion and activation processes in the gut lumen	552:640	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	8	80	gly	sialylated	1647:1656	arg1	ligands					1658:1664	sialylated ligands	1647:1664	sialylated ligands	1647:1664	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	3	81	dep	providing	422:430	arg1	addition					410:417	addition	410:417	addition	410:417	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	5	82	theme	rhamnosidase	1054:1065	arg1	activity					1067:1074	increased fucosidase and rhamnosidase activity	1029:1074	increased fucosidase and rhamnosidase activity	1029:1074	Whereas increased fucosidase and rhamnosidase activity did not alter the course of colitis, increased sialidase activity exacerbated disease severity.
36567940	4	83	theme	glycoside	812:820	arg1	hydrolase					822:830	local glycoside hydrolase	806:830	local glycoside hydrolase activity	806:839	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	7	84	theme	Increased	1399:1407	arg1	activity					1419:1426	Increased sialidase activity	1399:1426	Increased sialidase activity in the colon of treated mice	1399:1455	Increased sialidase activity in the colon of treated mice however significantly altered the distribution of sialic acid on mucosal glycans.
36567940	8	85	theme	ligands	1658:1664	arg1	density					1636:1642	the density	1632:1642	the density of sialylated ligands to anti-inflammatory siglec lectins	1632:1700	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	4	86	theme	colon	773:777	arg1	inflammation					779:790	colon inflammation	773:790	colon inflammation	773:790	To investigate the impact of bacterial glycoside hydrolase activities on the gut microbial composition and on host glycans during colon inflammation, we increased local glycoside hydrolase activity by supplementing mice with recombinant E. coli expressing specific sialidase, fucosidase and rhamnosidase enzymes during acute colitis induced by dextran sulfate sodium ingestion.
36567940	0	87	theme	bacterial	23:31	arg1	activity					43:50	intestinal bacterial sialidase activity	12:50	intestinal bacterial sialidase activity	12:50	Increase of intestinal bacterial sialidase activity exacerbates acute colitis in mice.
36567940	8	88	theme	anti-inflammatory	1669:1685	arg1	lectins					1694:1700	anti-inflammatory siglec lectins	1669:1700	anti-inflammatory siglec lectins	1669:1700	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	6	89	from	changes	1249:1255	arg1	composition					1274:1284	the microbial composition	1260:1284	the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli	1260:1396	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	3	90	theme	bacterial	493:501	arg1	hydrolases					513:522	bacterial glycoside hydrolases	493:522	bacterial glycoside hydrolases	493:522	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	0	91	theme	activity	43:50	arg1	Increase					0:7	Increase	0:7	Increase of intestinal bacterial sialidase activity	0:50	Increase of intestinal bacterial sialidase activity exacerbates acute colitis in mice.
36567940	3	92	theme	glycans	482:488	arg1	cleavage					465:472	the cleavage	461:472	the cleavage of host glycans by bacterial glycoside hydrolases	461:522	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
36567940	0	93	theme	acute	64:68	arg1	colitis					70:76	acute colitis	64:76	acute colitis in mice	64:84	Increase of intestinal bacterial sialidase activity exacerbates acute colitis in mice.
36567940	5	94	theme	colitis	1104:1110	arg1	course					1094:1099	the course	1090:1099	the course of colitis	1090:1110	Whereas increased fucosidase and rhamnosidase activity did not alter the course of colitis, increased sialidase activity exacerbated disease severity.
36567940	2	95	theme	Carbohydrate	224:235	arg1	foraging					237:244	Carbohydrate foraging	224:244	Carbohydrate foraging	224:244	Carbohydrate foraging requires the action of bacterially-encoded glycoside hydrolases, which release mono- and oligosaccharides taken up as carbon sources by multiple microbial taxa.
36567940	8	96	from	exacerbation	1829:1840	arg1	mice					1862:1865	mice	1862:1865	mice	1862:1865	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	8	97	theme	sialidase	1777:1785	arg1	activity					1787:1794	increased sialidase activity	1767:1794	increased sialidase activity	1767:1794	Treatment of lamina propria dendritic cells with bacterial sialidase also strongly decreased the density of sialylated ligands to anti-inflammatory siglec lectins, indicating that the remodeling of surface sialylation caused by increased sialidase activity likely accounts for the observed exacerbation of acute colitis in mice.
36567940	6	98	theme	sialidase	1196:1204	arg1	activity					1206:1213	increased sialidase activity	1186:1213	increased sialidase activity	1186:1213	The effect of increased sialidase activity on inflammation was not caused by changes in the microbial composition given that a similar shift in gut bacteria occurred in all groups of mice supplemented with recombinant E. coli.
36567940	3	99	theme	surface	552:558	arg1	glycoproteins					560:572	surface glycoproteins	552:572	surface glycoproteins involved in cell adhesion and activation processes in the gut lumen	552:640	In addition to providing nutrients to the microbiota, the cleavage of host glycans by bacterial glycoside hydrolases may alter the properties of surface glycoproteins involved in cell adhesion and activation processes in the gut lumen.
35832382	4	0	theme	natural	306:312	arg1	medicine					314:321	a natural medicine	304:321	a natural medicine	304:321	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	4	0	theme	natural	306:312	arg1	granule					287:293	leaf granule	282:293	leaf granule (COG)	282:299	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	11	1	theme	gut	1399:1401	arg1	microflora					1403:1412	gut microflora	1399:1412	gut microflora	1399:1412	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	9	2	theme	stress	1204:1209	arg1	damage					1211:1216	oxidative stress damage	1194:1216	oxidative stress damage in colitic mice	1194:1232	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	11	3	theme	colitic	1430:1436	arg1	mice					1438:1441	colitic mice	1430:1441	colitic mice	1430:1441	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	5	4	theme	ulcerative	476:485	arg1	colitis					487:493	ulcerative colitis	476:493	ulcerative colitis (UC)	476:498	However, the therapeutic effect of COG in ulcerative colitis (UC) has not been reported.
35832382	5	4	theme	ulcerative	476:485	arg1	UC					496:497	UC	496:497	UC	496:497	However, the therapeutic effect of COG in ulcerative colitis (UC) has not been reported.
35832382	4	5	theme	excellent	381:389	arg1	effects					425:431	excellent anti-inflammatory and antioxidant effects	381:431	excellent anti-inflammatory and antioxidant effects	381:431	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	7	6	theme	consecutive	703:713	arg1	days					715:718	11 consecutive days	700:718	11 consecutive days	700:718	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	11	7	dep	diversity	1363:1371	arg1	the					1359:1361	the	1359:1361	the	1359:1361	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	6	8	theme	dextran	571:577	arg1	DSS					595:597	DSS	595:597	DSS	595:597	Here, the experimental colitis was treated with dextran sodium sulfate (DSS) and COG.
35832382	6	8	theme	dextran	571:577	arg1	sulfate					586:592	dextran sodium sulfate	571:592	dextran sodium sulfate (DSS)	571:598	Here, the experimental colitis was treated with dextran sodium sulfate (DSS) and COG.
35832382	7	9	theme	body	725:728	arg1	weight					730:735	the body weight	721:735	the body weight	721:735	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	8	10	from	levels	900:905	arg1	mice					939:942	UC mice	936:942	UC mice	936:942	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	4	11	theme	diseases	361:368	arg1	treatment					336:344	the treatment	332:344	the treatment of respiratory diseases	332:368	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	5	12	theme	COG	469:471	arg1	effect					459:464	the therapeutic effect	443:464	the therapeutic effect of COG in ulcerative colitis (UC)	443:498	However, the therapeutic effect of COG in ulcerative colitis (UC) has not been reported.
35832382	11	13	from	diversity	1363:1371	arg1	colon					1421:1425	the colon	1417:1425	the colon of colitic mice	1417:1441	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	12	14	theme	flora	1762:1766	arg1	composition					1768:1778	intestinal flora composition	1751:1778	intestinal flora composition	1751:1778	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	11	15	theme	genus	1538:1542	arg1	level					1544:1548	the genus level	1534:1548	the genus level	1534:1548	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	9	16	theme	lower	1097:1101	arg1	levels					1103:1108	lower levels	1097:1108	lower levels of MDA, MPO, NO, and eNOS	1097:1134	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	2	17	theme	public	241:246	arg1	health					248:253	public health	241:253	public health	241:253	The rising incidence of ulcerative colitis has become a new challenge for public health.
35832382	1	18	theme	Microflora	144:153	arg1	Modulation					155:164	Gut Microflora Modulation	140:164	Gut Microflora Modulation	140:164	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	7	19	theme	pathological	816:827	arg1	score					829:833	the pathological score	812:833	the pathological score of mice	812:841	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	12	20	theme	mTreg	1726:1730	arg1	responses					1737:1745	mTreg cell responses	1726:1745	mTreg cell responses	1726:1745	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	7	21	theme	activity	746:753	arg1	DAI					762:764	DAI	762:764	DAI	762:764	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	21	theme	activity	746:753	arg1	index					755:759	disease activity index	738:759	disease activity index (DAI) score	738:771	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	8	22	theme	inflammatory	910:921	arg1	cytokines					923:931	inflammatory cytokines	910:931	inflammatory cytokines	910:931	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	1	23	theme	Treg	85:88	arg1	Improvement					95:105	Treg Cell Improvement	85:105	Treg Cell Improvement	85:105	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	9	24	theme	eNOS	1131:1134	arg1	levels					1147:1152	higher levels	1140:1152	higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG	1140:1254	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	9	24	theme	eNOS	1131:1134	arg1	levels					1103:1108	lower levels	1097:1108	lower levels of MDA, MPO, NO, and eNOS	1097:1134	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	12	25	theme	stress	1701:1706	arg1	regulation					1712:1721	regulation	1712:1721	regulation of mTreg cell responses and intestinal flora composition	1712:1778	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	25	theme	stress	1701:1706	arg1	inhibition					1643:1652	inhibition	1643:1652	inhibition of immune-inflammatory responses and oxidative stress	1643:1706	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	7	26	theme	colon	788:792	arg1	index					801:805	colon weight index	788:805	colon weight index	788:805	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	9	27	theme	higher	1140:1145	arg1	levels					1147:1152	higher levels	1140:1152	higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG	1140:1254	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	1	28	theme	Oxidative	108:116	arg1	Reduction					125:133	Oxidative Stress Reduction	108:133	Oxidative Stress Reduction	108:133	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	4	29	theme	leaf	282:285	arg1	medicine					314:321	a natural medicine	304:321	a natural medicine	304:321	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	4	29	theme	leaf	282:285	arg1	COG					296:298	COG	296:298	COG	296:298	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	4	29	theme	leaf	282:285	arg1	granule					287:293	leaf granule	282:293	leaf granule (COG)	282:299	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	12	30	theme	immune-inflammatory	1657:1675	arg1	responses					1677:1685	immune-inflammatory responses	1657:1685	immune-inflammatory responses	1657:1685	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	31	theme	experimental	1606:1617	arg1	colitis					1619:1625	DSS-induced experimental colitis	1594:1625	DSS-induced experimental colitis	1594:1625	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	32	from	effect	1584:1589	arg1	colitis					1619:1625	DSS-induced experimental colitis	1594:1625	DSS-induced experimental colitis	1594:1625	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	2	33	theme	ulcerative	191:200	arg1	colitis					202:208	ulcerative colitis	191:208	ulcerative colitis	191:208	The rising incidence of ulcerative colitis has become a new challenge for public health.
35832382	7	34	theme	colon	774:778	arg1	length					780:785	colon length	774:785	colon length	774:785	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	1	35	theme	DSS-Induced	51:61	arg1	Colitis					69:75	DSS-Induced Acute Colitis	51:75	DSS-Induced Acute Colitis	51:75	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	12	36	theme	protective	1573:1582	arg1	effect					1584:1589	a protective effect	1571:1589	a protective effect on DSS-induced experimental colitis	1571:1625	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	7	37	theme	COG	692:694	arg1	high					630:633	high	630:633	high	630:633	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	37	theme	COG	692:694	arg1	30 g/kg					636:642	30 g/kg	636:642	30 g/kg	636:642	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	11	38	theme	relative	1377:1384	arg1	abundance					1386:1394	relative abundance	1377:1394	relative abundance	1377:1394	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	9	39	theme	oxidative	1194:1202	arg1	damage					1211:1216	oxidative stress damage	1194:1216	oxidative stress damage in colitic mice	1194:1232	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	11	40	theme	microflora	1403:1412	arg1	diversity					1363:1371	diversity	1363:1371	diversity	1363:1371	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	11	40	theme	microflora	1403:1412	arg1	abundance					1386:1394	relative abundance	1377:1394	relative abundance	1377:1394	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	8	41	theme	IL-6	1002:1005	arg1	levels					992:997	the secretion levels	978:997	the secretion levels of IL-6 and IL-10 in the colon	978:1028	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	4	42	theme	anti-inflammatory	391:407	arg1	effects					425:431	excellent anti-inflammatory and antioxidant effects	381:431	excellent anti-inflammatory and antioxidant effects	381:431	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	11	43	theme	mice	1438:1441	arg1	colon					1421:1425	the colon	1417:1425	the colon of colitic mice	1417:1441	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	7	44	theme	index	755:759	arg1	score					767:771	disease activity index (DAI) score	738:771	disease activity index (DAI) score	738:771	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	9	45	theme	colitic	1221:1227	arg1	mice					1229:1232	colitic mice	1221:1232	colitic mice	1221:1232	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	8	46	theme	IL-10	1011:1015	arg1	levels					992:997	the secretion levels	978:997	the secretion levels of IL-6 and IL-10 in the colon	978:1028	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	4	47	theme	respiratory	349:359	arg1	diseases					361:368	respiratory diseases	349:368	respiratory diseases	349:368	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	6	48	theme	sodium	579:584	arg1	DSS					595:597	DSS	595:597	DSS	595:597	Here, the experimental colitis was treated with dextran sodium sulfate (DSS) and COG.
35832382	6	48	theme	sodium	579:584	arg1	sulfate					586:592	dextran sodium sulfate	571:592	dextran sodium sulfate (DSS)	571:598	Here, the experimental colitis was treated with dextran sodium sulfate (DSS) and COG.
35832382	7	49	theme	disease	738:744	arg1	DAI					762:764	DAI	762:764	DAI	762:764	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	49	theme	disease	738:744	arg1	index					755:759	disease activity index	738:759	disease activity index (DAI) score	738:771	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	11	50	from	abundance	1386:1394	arg1	colon					1421:1425	the colon	1417:1425	the colon of colitic mice	1417:1441	In addition, COG improved the diversity and relative abundance of gut microflora in the colon of colitic mice, and Lachnospiraceae_NK4A136_group and Lachnospiraceae_UCG-006 were obviously regulated at the genus level.
35832382	12	51	theme	composition	1768:1778	arg1	regulation					1712:1721	regulation	1712:1721	regulation of mTreg cell responses and intestinal flora composition	1712:1778	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	51	theme	composition	1768:1778	arg1	inhibition					1643:1652	inhibition	1643:1652	inhibition of immune-inflammatory responses and oxidative stress	1643:1706	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	52	contain	has	1567:1569	arg1	COG					1563:1565	COG	1563:1565	COG	1563:1565	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	52	contain	has	1567:1569	arg2	effect					1584:1589	a protective effect	1571:1589	a protective effect on DSS-induced experimental colitis	1571:1625	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	53	theme	responses	1737:1745	arg1	regulation					1712:1721	regulation	1712:1721	regulation of mTreg cell responses and intestinal flora composition	1712:1778	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	53	theme	responses	1737:1745	arg1	inhibition					1643:1652	inhibition	1643:1652	inhibition of immune-inflammatory responses and oxidative stress	1643:1706	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	6	54	theme	experimental	533:544	arg1	colitis					546:552	the experimental colitis	529:552	the experimental colitis	529:552	Here, the experimental colitis was treated with dextran sodium sulfate (DSS) and COG.
35832382	9	55	theme	COG-treated	1073:1083	arg1	mice					1085:1088	COG-treated mice	1073:1088	COG-treated mice	1073:1088	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	2	56	theme	new	223:225	arg1	challenge					227:235	a new challenge	221:235	a new challenge for public health	221:253	The rising incidence of ulcerative colitis has become a new challenge for public health.
35832382	1	57	theme	Stress	118:123	arg1	Reduction					125:133	Oxidative Stress Reduction	108:133	Oxidative Stress Reduction	108:133	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	12	58	theme	intestinal	1751:1760	arg1	composition					1768:1778	intestinal flora composition	1751:1778	intestinal flora composition	1751:1778	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	4	59	theme	antioxidant	413:423	arg1	effects					425:431	excellent anti-inflammatory and antioxidant effects	381:431	excellent anti-inflammatory and antioxidant effects	381:431	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	10	60	located	observed	1297:1304	arg2	Tregs					1286:1290	less Th17 and more Tregs	1267:1290	less Th17 and more Tregs	1267:1290	Moreover, less Th17 and more Tregs were observed in the COG-treated groups.
35832382	10	60	located	observed	1297:1304	arg1	groups					1325:1330	the COG-treated groups	1309:1330	the COG-treated groups	1309:1330	Moreover, less Th17 and more Tregs were observed in the COG-treated groups.
35832382	1	61	theme	Gut	140:142	arg1	Microflora					144:153	Gut Microflora	140:153	Gut Microflora Modulation	140:164	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	9	62	theme	MDA	1113:1115	arg1	levels					1147:1152	higher levels	1140:1152	higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG	1140:1254	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	9	62	theme	MDA	1113:1115	arg1	levels					1103:1108	lower levels	1097:1108	lower levels of MDA, MPO, NO, and eNOS	1097:1134	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	7	63	theme	mice	838:841	arg1	index					801:805	colon weight index	788:805	colon weight index	788:805	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	63	theme	mice	838:841	arg1	score					767:771	disease activity index (DAI) score	738:771	disease activity index (DAI) score	738:771	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	63	theme	mice	838:841	arg1	weight					730:735	the body weight	721:735	the body weight	721:735	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	63	theme	mice	838:841	arg1	length					780:785	colon length	774:785	colon length	774:785	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	63	theme	mice	838:841	arg1	score					829:833	the pathological score	812:833	the pathological score of mice	812:841	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	9	64	theme	MPO	1118:1120	arg1	levels					1147:1152	higher levels	1140:1152	higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG	1140:1254	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	9	64	theme	MPO	1118:1120	arg1	levels					1103:1108	lower levels	1097:1108	lower levels of MDA, MPO, NO, and eNOS	1097:1134	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	12	65	theme	cell	1732:1735	arg1	responses					1737:1745	mTreg cell responses	1726:1745	mTreg cell responses	1726:1745	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	1	66	theme	Acute	63:67	arg1	Colitis					69:75	DSS-Induced Acute Colitis	51:75	DSS-Induced Acute Colitis	51:75	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	7	67	with	treatment	615:623	arg1	high					630:633	high	630:633	high	630:633	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	67	with	treatment	615:623	arg1	15 g/kg					654:660	15 g/kg	654:660	15 g/kg	654:660	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	67	with	treatment	615:623	arg1	doses					683:687	low (7.5 g/kg) doses	668:687	low (7.5 g/kg) doses	668:687	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	67	with	treatment	615:623	arg1	30 g/kg					636:642	30 g/kg	636:642	30 g/kg	636:642	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	7	67	with	treatment	615:623	arg1	medium					646:651	medium	646:651	medium (15 g/kg)	646:661	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	9	68	theme	NO	1123:1124	arg1	levels					1147:1152	higher levels	1140:1152	higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG	1140:1254	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	9	68	theme	NO	1123:1124	arg1	levels					1103:1108	lower levels	1097:1108	lower levels of MDA, MPO, NO, and eNOS	1097:1134	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	8	69	theme	cytokines	923:931	arg1	levels					900:905	the levels	896:905	the levels of inflammatory cytokines in UC mice	896:942	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	8	70	theme	UC	936:937	arg1	mice					939:942	UC mice	936:942	UC mice	936:942	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	1	71	theme	Cell	90:93	arg1	Improvement					95:105	Treg Cell Improvement	85:105	Treg Cell Improvement	85:105	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	12	72	theme	oxidative	1691:1699	arg1	stress					1701:1706	oxidative stress	1691:1706	oxidative stress	1691:1706	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	9	73	with	mice	1054:1057	arg1	colitis					1064:1070	colitis	1064:1070	colitis	1064:1070	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	9	74	from	damage	1211:1216	arg1	mice					1229:1232	colitic mice	1221:1232	colitic mice	1221:1232	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	7	75	theme	weight	794:799	arg1	index					801:805	colon weight index	788:805	colon weight index	788:805	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	12	76	theme	responses	1677:1685	arg1	regulation					1712:1721	regulation	1712:1721	regulation of mTreg cell responses and intestinal flora composition	1712:1778	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	12	76	theme	responses	1677:1685	arg1	inhibition					1643:1652	inhibition	1643:1652	inhibition of immune-inflammatory responses and oxidative stress	1643:1706	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	8	77	from	levels	992:997	arg1	colon					1024:1028	the colon	1020:1028	the colon	1020:1028	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	5	78	theme	therapeutic	447:457	arg1	effect					459:464	the therapeutic effect	443:464	the therapeutic effect of COG in ulcerative colitis (UC)	443:498	However, the therapeutic effect of COG in ulcerative colitis (UC) has not been reported.
35832382	2	79	theme	rising	171:176	arg1	incidence					178:186	The rising incidence	167:186	The rising incidence of ulcerative colitis	167:208	The rising incidence of ulcerative colitis has become a new challenge for public health.
35832382	9	80	theme	GSH-Px	1157:1162	arg1	levels					1147:1152	higher levels	1140:1152	higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG	1140:1254	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	9	80	theme	GSH-Px	1157:1162	arg1	levels					1103:1108	lower levels	1097:1108	lower levels of MDA, MPO, NO, and eNOS	1097:1134	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	5	81	from	effect	459:464	arg1	colitis					487:493	ulcerative colitis	476:493	ulcerative colitis (UC)	476:498	However, the therapeutic effect of COG in ulcerative colitis (UC) has not been reported.
35832382	5	81	from	effect	459:464	arg1	UC					496:497	UC	496:497	UC	496:497	However, the therapeutic effect of COG in ulcerative colitis (UC) has not been reported.
35832382	4	82	contain	has	377:379	arg1	medicine					314:321	a natural medicine	304:321	a natural medicine	304:321	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	4	82	contain	has	377:379	arg1	granule					287:293	leaf granule	282:293	leaf granule (COG)	282:299	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	4	82	contain	has	377:379	arg2	effects					425:431	excellent anti-inflammatory and antioxidant effects	381:431	excellent anti-inflammatory and antioxidant effects	381:431	leaf granule (COG) is a natural medicine used for the treatment of respiratory diseases, which has excellent anti-inflammatory and antioxidant effects.
35832382	9	83	theme	MAO	1168:1170	arg1	levels					1147:1152	higher levels	1140:1152	higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG	1140:1254	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	9	83	theme	MAO	1168:1170	arg1	levels					1103:1108	lower levels	1097:1108	lower levels of MDA, MPO, NO, and eNOS	1097:1134	Meanwhile, compared to mice with colitis, COG-treated mice showed lower levels of MDA, MPO, NO, and eNOS and higher levels of GSH-Px and MAO, which indicated that oxidative stress damage in colitic mice was alleviated by COG.
35832382	1	84	theme	Leaf	26:29	arg1	Granule					31:37	Leaf Granule	26:37	Leaf Granule	26:37	Leaf Granule Ameliorates DSS-Induced Acute Colitis Through Treg Cell Improvement, Oxidative Stress Reduction, and Gut Microflora Modulation.
35832382	12	85	theme	DSS-induced	1594:1604	arg1	colitis					1619:1625	DSS-induced experimental colitis	1594:1625	DSS-induced experimental colitis	1594:1625	In summary, COG has a protective effect on DSS-induced experimental colitis, mainly through inhibition of immune-inflammatory responses and oxidative stress and regulation of mTreg cell responses and intestinal flora composition.
35832382	2	86	theme	colitis	202:208	arg1	incidence					178:186	The rising incidence	167:186	The rising incidence of ulcerative colitis	167:208	The rising incidence of ulcerative colitis has become a new challenge for public health.
35832382	7	87	theme	7.5 g/kg	673:680	arg1	doses					683:687	low (7.5 g/kg) doses	668:687	low (7.5 g/kg) doses	668:687	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35832382	8	88	theme	secretion	982:990	arg1	levels					992:997	the secretion levels	978:997	the secretion levels of IL-6 and IL-10 in the colon	978:1028	COG significantly reduced the levels of inflammatory cytokines in UC mice in vitro and in vivo and restored the secretion levels of IL-6 and IL-10 in the colon.
35832382	10	89	theme	COG-treated	1313:1323	arg1	groups					1325:1330	the COG-treated groups	1309:1330	the COG-treated groups	1309:1330	Moreover, less Th17 and more Tregs were observed in the COG-treated groups.
35832382	7	90	theme	low	668:670	arg1	doses					683:687	low (7.5 g/kg) doses	668:687	low (7.5 g/kg) doses	668:687	After treatment with high (30 g/kg), medium (15 g/kg), and low (7.5 g/kg) doses of COG for 11 consecutive days, the body weight, disease activity index (DAI) score, colon length, colon weight index, and the pathological score of mice were effectively improved.
35697846	10	0	theme	immune	1511:1516	arg1	responses					1518:1526	immune responses	1511:1526	immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice	1511:1637	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	8	1	theme	derived	1082:1088	arg1	protein					1100:1106	plant derived rGA733-Fc protein	1076:1106	plant derived rGA733-Fc protein	1076:1106	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	5	2	theme	edible	589:594	arg1	vaccine					614:620	an edible colorectal cancer vaccine	586:620	an edible colorectal cancer vaccine	586:620	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	8	3	theme	typical	1162:1168	arg1	pattern					1184:1190	a typical glycosylation pattern	1160:1190	a typical glycosylation pattern found on ER-processing proteins	1160:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	3	theme	typical	1162:1168	arg1	structure					1140:1148	an oligomannose glycan structure	1117:1148	an oligomannose glycan structure	1117:1148	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	10	4	theme	oral	1540:1543	arg1	administration					1545:1558	oral administration	1540:1558	oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice	1540:1637	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	7	5	theme	rGA733-Fc	1004:1012	arg1	protein					1014:1020	rGA733-Fc protein	1004:1020	rGA733-Fc protein	1004:1020	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	10	6	theme	Oral	1380:1383	arg1	administration					1385:1398	Oral administration	1380:1398	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants	1380:1461	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	2	7	theme	colorectal	254:263	arg1	CRC					276:278	CRC	276:278	CRC	276:278	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	2	7	theme	colorectal	254:263	arg1	carcinoma					265:273	colorectal carcinoma	254:273	colorectal carcinoma (CRC)-associated antigen	254:298	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	7	8	theme	level	995:999	arg1	integration					935:945	stable integration	928:945	stable integration of transgene expression cassette and expression level of rGA733-Fc protein	928:1020	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	8	9	theme	glycan	1133:1138	arg1	pattern					1184:1190	a typical glycosylation pattern	1160:1190	a typical glycosylation pattern found on ER-processing proteins	1160:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	9	theme	glycan	1133:1138	arg1	structure					1140:1148	an oligomannose glycan structure	1117:1148	an oligomannose glycan structure	1117:1148	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	10	10	theme	fruits	1570:1575	arg1	administration					1545:1558	oral administration	1540:1558	oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice	1540:1637	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	5	11	theme	transgene	627:635	arg1	domain					722:727	the fragment crystallizable (Fc) domain	689:727	the fragment crystallizable (Fc) domain	689:727	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	11	theme	transgene	627:635	arg1	motif					751:755	the ER retention motif	734:755	the ER retention motif (rGA733-Fc)	734:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	11	theme	transgene	627:635	arg1	protein					669:675	a fusion protein	660:675	a fusion protein of GA733-2	660:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	11	theme	transgene	627:635	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	11	theme	transgene	627:635	arg1	GA733-2					680:686	GA733-2	680:686	GA733-2	680:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	12	theme	ER	738:739	arg1	rGA733-Fc					758:766	rGA733-Fc	758:766	rGA733-Fc	758:766	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	12	theme	ER	738:739	arg1	motif					751:755	the ER retention motif	734:755	the ER retention motif (rGA733-Fc)	734:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	12	theme	ER	738:739	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	3	13	theme	fresh	472:476	arg1	form					478:481	fresh form	472:481	fresh form	472:481	Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form.
35697846	3	14	theme	major	388:392	arg1	targets					394:400	the major targets	384:400	the major targets for production of an edible vaccine	384:436	Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form.
35697846	7	15	theme	expression	960:969	arg1	cassette					971:978	transgene expression cassette	950:978	transgene expression cassette	950:978	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	10	16	theme	tomato	1563:1568	arg1	fruits					1570:1575	tomato fruits	1563:1575	tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice	1563:1637	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	5	17	theme	cancer	607:612	arg1	vaccine					614:620	an edible colorectal cancer vaccine	586:620	an edible colorectal cancer vaccine	586:620	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	18	theme	Solanum	804:810	arg1	cv					825:826	Solanum lycopersicum cv	804:826	Solanum lycopersicum cv	804:826	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	7	19	theme	stable	928:933	arg1	integration					935:945	stable integration	928:945	stable integration of transgene expression cassette and expression level of rGA733-Fc protein	928:1020	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	7	20	theme	rGA733-FcOX	884:894	arg1	protein					897:903	rGA733-Fc (rGA733-FcOX) protein	873:903	rGA733-Fc (rGA733-FcOX) protein	873:903	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	11	21	theme	colorectal	1711:1720	arg1	vaccine					1729:1735	an edible colorectal cancer vaccine	1701:1735	an edible colorectal cancer vaccine using tomato plants	1701:1755	This is the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants.
35697846	5	22	theme	GA733-2	680:686	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	22	theme	GA733-2	680:686	arg1	GA733-2					680:686	GA733-2	680:686	GA733-2	680:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	22	theme	GA733-2	680:686	arg1	motif					751:755	the ER retention motif	734:755	the ER retention motif (rGA733-Fc)	734:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	22	theme	GA733-2	680:686	arg1	domain					722:727	the fragment crystallizable (Fc) domain	689:727	the fragment crystallizable (Fc) domain	689:727	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	22	theme	GA733-2	680:686	arg1	rGA733-Fc					758:766	rGA733-Fc	758:766	rGA733-Fc	758:766	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	22	theme	GA733-2	680:686	arg1	protein					669:675	a fusion protein	660:675	a fusion protein of GA733-2	660:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	8	23	link	derived	1082:1088	arg1	protein					1100:1106	plant derived rGA733-Fc protein	1076:1106	plant derived rGA733-Fc protein	1076:1106	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	10	24	theme	fruits	1410:1415	arg1	administration					1385:1398	Oral administration	1380:1398	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants	1380:1461	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	9	25	theme	red	1229:1231	arg1	fruits					1233:1238	The red fruits	1225:1238	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein	1225:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	8	26	dep	derived	1082:1088	arg1	plant					1076:1080	plant	1076:1080	plant	1076:1080	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	11	27	theme	tomato	1743:1748	arg1	plants					1750:1755	tomato plants	1743:1755	tomato plants	1743:1755	This is the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants.
35697846	3	28	theme	targets	394:400	arg1	targets					394:400	the major targets	384:400	the major targets for production of an edible vaccine	384:436	Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form.
35697846	3	28	theme	targets	394:400	arg1	Tomato					363:368	Tomato	363:368	Tomato	363:368	Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form.
35697846	3	28	theme	targets	394:400	arg1	one					377:379	one	377:379	one	377:379	Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form.
35697846	9	29	theme	transgenic	1255:1264	arg1	plants					1273:1278	rGA733-FcOX transgenic tomato plants	1243:1278	rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein	1243:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	0	30	theme	cancer	52:57	arg1	antigen					59:65	recombinant colorectal cancer antigen	29:65	recombinant colorectal cancer antigen	29:65	Immunotherapeutic effects of recombinant colorectal cancer antigen produced in tomato fruits.
35697846	10	31	theme	C57BL/6J	1625:1632	arg1	mice					1634:1637	the C57BL/6J mice	1621:1637	the C57BL/6J mice	1621:1637	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	0	32	theme	Immunotherapeutic	0:16	arg1	effects					18:24	Immunotherapeutic effects	0:24	Immunotherapeutic effects of recombinant colorectal cancer antigen	0:65	Immunotherapeutic effects of recombinant colorectal cancer antigen produced in tomato fruits.
35697846	9	33	theme	plants	1273:1278	arg1	fruits					1233:1238	The red fruits	1225:1238	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein	1225:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	10	34	theme	colorectal	1471:1480	arg1	growth					1489:1494	colorectal cancer growth	1471:1494	colorectal cancer growth	1471:1494	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	1	35	theme	pharmacological	112:126	arg1	vaccines					128:135	pharmacological vaccines	112:135	pharmacological vaccines	112:135	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	0	36	theme	colorectal	41:50	arg1	antigen					59:65	recombinant colorectal cancer antigen	29:65	recombinant colorectal cancer antigen	29:65	Immunotherapeutic effects of recombinant colorectal cancer antigen produced in tomato fruits.
35697846	5	37	theme	crystallizable	702:715	arg1	domain					722:727	the fragment crystallizable (Fc) domain	689:727	the fragment crystallizable (Fc) domain	689:727	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	37	theme	crystallizable	702:715	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	37	theme	crystallizable	702:715	arg1	GA733-2					680:686	GA733-2	680:686	GA733-2	680:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	38	theme	Fc	718:719	arg1	domain					722:727	the fragment crystallizable (Fc) domain	689:727	the fragment crystallizable (Fc) domain	689:727	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	38	theme	Fc	718:719	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	38	theme	Fc	718:719	arg1	GA733-2					680:686	GA733-2	680:686	GA733-2	680:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	9	39	theme	rGA733-Fc	1320:1328	arg1	protein					1330:1336	rGA733-Fc protein	1320:1336	rGA733-Fc protein	1320:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	12	40	theme	cancer	1822:1827	arg1	vaccines					1836:1843	plant-derived cancer edible vaccines	1808:1843	plant-derived cancer edible vaccines	1808:1843	This research would be helpful for development of plant-derived cancer edible vaccines.
35697846	12	41	theme	plant-derived	1808:1820	arg1	vaccines					1836:1843	plant-derived cancer edible vaccines	1808:1843	plant-derived cancer edible vaccines	1808:1843	This research would be helpful for development of plant-derived cancer edible vaccines.
35697846	11	42	theme	edible	1704:1709	arg1	vaccine					1729:1735	an edible colorectal cancer vaccine	1701:1735	an edible colorectal cancer vaccine using tomato plants	1701:1755	This is the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants.
35697846	3	43	theme	vaccine	430:436	arg1	production					406:415	production	406:415	production of an edible vaccine	406:436	Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form.
35697846	7	44	theme	rGA733-Fc	873:881	arg1	protein					897:903	rGA733-Fc (rGA733-FcOX) protein	873:903	rGA733-Fc (rGA733-FcOX) protein	873:903	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	8	45	theme	Further	1023:1029	arg1	analysis					1053:1060	Further glycosylation pattern analysis	1023:1060	Further glycosylation pattern analysis	1023:1060	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	2	46	theme	colorectal	336:345	arg1	vaccine					354:360	a colorectal cancer vaccine	334:360	a colorectal cancer vaccine	334:360	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	1	47	from	production	98:107	arg1	plants					140:145	plants	140:145	plants	140:145	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	8	48	theme	pattern	1045:1051	arg1	analysis					1053:1060	Further glycosylation pattern analysis	1023:1060	Further glycosylation pattern analysis	1023:1060	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	9	49	theme	C57BL/6	1366:1372	arg1	mice					1374:1377	C57BL/6 mice	1366:1377	C57BL/6 mice	1366:1377	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	1	50	theme	biotechnology	196:208	arg1	field					181:185	the field	177:185	the field of plant biotechnology	177:208	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	7	51	theme	protein	1014:1020	arg1	cassette					971:978	transgene expression cassette	950:978	transgene expression cassette	950:978	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	7	51	theme	protein	1014:1020	arg1	level					995:999	expression level	984:999	expression level of rGA733-Fc protein	984:1020	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	5	52	theme	tomato	789:794	arg1	plants					796:801	tomato plants	789:801	tomato plants (Solanum lycopersicum cv	789:826	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	4	53	theme	vitamins	517:524	arg1	content					506:512	high content	501:512	high content of vitamins that aid activation of immune response	501:563	It also contains high content of vitamins that aid activation of immune response.
35697846	11	54	theme	first	1652:1656	arg1	study					1658:1662	the first study	1648:1662	the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants	1648:1755	This is the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants.
35697846	11	54	theme	first	1652:1656	arg1	This					1640:1643	This	1640:1643	This	1640:1643	This is the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants.
35697846	2	55	theme	carcinoma	265:273	arg1	protein					224:230	the protein	220:230	the protein that is also known as colorectal carcinoma (CRC)-associated antigen	220:298	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	2	55	theme	carcinoma	265:273	arg1	antigen					292:298	colorectal carcinoma (CRC)-associated antigen	254:298	colorectal carcinoma (CRC)-associated antigen	254:298	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	8	56	theme	rGA733-Fc	1090:1098	arg1	protein					1100:1106	plant derived rGA733-Fc protein	1076:1106	plant derived rGA733-Fc protein	1076:1106	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	10	57	theme	h-Fc	1584:1587	arg1	fruits					1570:1575	tomato fruits	1563:1575	tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice	1563:1637	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	7	58	theme	expression	984:993	arg1	level					995:999	expression level	984:999	expression level of rGA733-Fc protein	984:1020	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	4	59	contain	contains	492:499	arg2	content					506:512	high content	501:512	high content of vitamins that aid activation of immune response	501:563	It also contains high content of vitamins that aid activation of immune response.
35697846	4	59	contain	contains	492:499	arg1	It					484:485	It	484:485	It	484:485	It also contains high content of vitamins that aid activation of immune response.
35697846	0	60	theme	antigen	59:65	arg1	effects					18:24	Immunotherapeutic effects	0:24	Immunotherapeutic effects of recombinant colorectal cancer antigen	0:65	Immunotherapeutic effects of recombinant colorectal cancer antigen produced in tomato fruits.
35697846	8	61	theme	glycosylation	1170:1182	arg1	pattern					1184:1190	a typical glycosylation pattern	1160:1190	a typical glycosylation pattern found on ER-processing proteins	1160:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	61	theme	glycosylation	1170:1182	arg1	structure					1140:1148	an oligomannose glycan structure	1117:1148	an oligomannose glycan structure	1117:1148	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	7	62	theme	cassette	971:978	arg1	integration					935:945	stable integration	928:945	stable integration of transgene expression cassette and expression level of rGA733-Fc protein	928:1020	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	2	63	theme	-associated	280:290	arg1	protein					224:230	the protein	220:230	the protein that is also known as colorectal carcinoma (CRC)-associated antigen	220:298	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	2	63	theme	-associated	280:290	arg1	antigen					292:298	colorectal carcinoma (CRC)-associated antigen	254:298	colorectal carcinoma (CRC)-associated antigen	254:298	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	1	64	theme	plant	190:194	arg1	biotechnology					196:208	plant biotechnology	190:208	plant biotechnology	190:208	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	8	65	theme	oligomannose	1120:1131	arg1	pattern					1184:1190	a typical glycosylation pattern	1160:1190	a typical glycosylation pattern found on ER-processing proteins	1160:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	65	theme	oligomannose	1120:1131	arg1	structure					1140:1148	an oligomannose glycan structure	1117:1148	an oligomannose glycan structure	1117:1148	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	4	66	theme	response	556:563	arg1	activation					535:544	activation	535:544	activation of immune response	535:563	It also contains high content of vitamins that aid activation of immune response.
35697846	7	67	theme	transgene	950:958	arg1	cassette					971:978	transgene expression cassette	950:978	transgene expression cassette	950:978	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	5	68	theme	retention	741:749	arg1	rGA733-Fc					758:766	rGA733-Fc	758:766	rGA733-Fc	758:766	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	68	theme	retention	741:749	arg1	motif					751:755	the ER retention motif	734:755	the ER retention motif (rGA733-Fc)	734:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	68	theme	retention	741:749	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	8	69	theme	ER-processing	1201:1213	arg1	proteins					1215:1222	ER-processing proteins	1201:1222	ER-processing proteins	1201:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	10	70	theme	transgenic	1600:1609	arg1	plants					1611:1616	transgenic plants	1600:1616	transgenic plants	1600:1616	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	5	71	theme	colorectal	596:605	arg1	vaccine					614:620	an edible colorectal cancer vaccine	586:620	an edible colorectal cancer vaccine	586:620	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	1	72	theme	vaccines	128:135	arg1	production					98:107	The production	94:107	The production of pharmacological vaccines in plants	94:145	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	1	72	theme	vaccines	128:135	arg1	goal					169:172	an important goal	156:172	an important goal	156:172	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	5	73	theme	lycopersicum	812:823	arg1	cv					825:826	Solanum lycopersicum cv	804:826	Solanum lycopersicum cv	804:826	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	0	74	theme	tomato	79:84	arg1	fruits					86:91	tomato fruits	79:91	tomato fruits	79:91	Immunotherapeutic effects of recombinant colorectal cancer antigen produced in tomato fruits.
35697846	8	75	contain	contains	1108:1115	arg2	pattern					1184:1190	a typical glycosylation pattern	1160:1190	a typical glycosylation pattern found on ER-processing proteins	1160:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	75	contain	contains	1108:1115	arg2	structure					1140:1148	an oligomannose glycan structure	1117:1148	an oligomannose glycan structure	1117:1148	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	75	contain	contains	1108:1115	arg1	protein					1100:1106	plant derived rGA733-Fc protein	1076:1106	plant derived rGA733-Fc protein	1076:1106	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	11	76	theme	cancer	1722:1727	arg1	vaccine					1729:1735	an edible colorectal cancer vaccine	1701:1735	an edible colorectal cancer vaccine using tomato plants	1701:1755	This is the first study showing the possibility of producing an edible colorectal cancer vaccine using tomato plants.
35697846	12	77	theme	vaccines	1836:1843	arg1	development					1793:1803	development	1793:1803	development of plant-derived cancer edible vaccines	1793:1843	This research would be helpful for development of plant-derived cancer edible vaccines.
35697846	1	78	theme	important	159:167	arg1	production					98:107	The production	94:107	The production of pharmacological vaccines in plants	94:145	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	1	78	theme	important	159:167	arg1	goal					169:172	an important goal	156:172	an important goal	156:172	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	10	79	theme	tomato	1403:1408	arg1	fruits					1410:1415	tomato fruits	1403:1415	tomato fruits	1403:1415	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	1	80	from	goal	169:172	arg1	field					181:185	the field	177:185	the field of plant biotechnology	177:208	The production of pharmacological vaccines in plants has been an important goal in the field of plant biotechnology.
35697846	5	81	theme	fusion	662:667	arg1	protein					669:675	a fusion protein	660:675	a fusion protein of GA733-2	660:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	81	theme	fusion	662:667	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	9	82	theme	rGA733-FcOX	1243:1253	arg1	plants					1273:1278	rGA733-FcOX transgenic tomato plants	1243:1278	rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein	1243:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	8	83	located	found	1192:1196	arg2	pattern					1184:1190	a typical glycosylation pattern	1160:1190	a typical glycosylation pattern found on ER-processing proteins	1160:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	83	located	found	1192:1196	arg2	structure					1140:1148	an oligomannose glycan structure	1117:1148	an oligomannose glycan structure	1117:1148	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	8	83	located	found	1192:1196	arg1	proteins					1215:1222	ER-processing proteins	1201:1222	ER-processing proteins	1201:1222	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	7	84	theme	transgenic	845:854	arg1	plants					856:861	The transgenic plants	841:861	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein	841:903	The transgenic plants producing rGA733-Fc (rGA733-FcOX) protein were screened based on stable integration of transgene expression cassette and expression level of rGA733-Fc protein.
35697846	10	85	theme	rGA733-Fc	1424:1432	arg1	administration					1385:1398	Oral administration	1380:1398	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants	1380:1461	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	12	86	link	plant-derived	1808:1820	arg1	vaccines					1836:1843	plant-derived cancer edible vaccines	1808:1843	plant-derived cancer edible vaccines	1808:1843	This research would be helpful for development of plant-derived cancer edible vaccines.
35697846	9	87	theme	tomato	1266:1271	arg1	plants					1273:1278	rGA733-FcOX transgenic tomato plants	1243:1278	rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein	1243:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	5	88	dep	plants	796:801	arg1	cv					825:826	Solanum lycopersicum cv	804:826	Solanum lycopersicum cv	804:826	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	2	89	theme	cancer	347:352	arg1	vaccine					354:360	a colorectal cancer vaccine	334:360	a colorectal cancer vaccine	334:360	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	0	90	theme	recombinant	29:39	arg1	antigen					59:65	recombinant colorectal cancer antigen	29:65	recombinant colorectal cancer antigen	29:65	Immunotherapeutic effects of recombinant colorectal cancer antigen produced in tomato fruits.
35697846	4	91	theme	high	501:504	arg1	content					506:512	high content	501:512	high content of vitamins that aid activation of immune response	501:563	It also contains high content of vitamins that aid activation of immune response.
35697846	3	92	theme	edible	423:428	arg1	vaccine					430:436	an edible vaccine	420:436	an edible vaccine	420:436	Tomato is the one of the major targets for production of an edible vaccine, as tomato is a fruit consumed in fresh form.
35697846	5	93	theme	fragment	693:700	arg1	domain					722:727	the fragment crystallizable (Fc) domain	689:727	the fragment crystallizable (Fc) domain	689:727	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	93	theme	fragment	693:700	arg1	rGA733-Fc					637:645	the transgene rGA733-Fc	623:645	the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc)	623:767	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	5	93	theme	fragment	693:700	arg1	GA733-2					680:686	GA733-2	680:686	GA733-2	680:686	In order to develop an edible colorectal cancer vaccine, the transgene rGA733-Fc that encodes a fusion protein of GA733-2, the fragment crystallizable (Fc) domain, and the ER retention motif (rGA733-Fc) was introduced into tomato plants (Solanum lycopersicum cv.
35697846	10	94	theme	transgenic	1445:1454	arg1	plants					1456:1461	transgenic plants	1445:1461	transgenic plants	1445:1461	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	12	95	theme	edible	1829:1834	arg1	vaccines					1836:1843	plant-derived cancer edible vaccines	1808:1843	plant-derived cancer edible vaccines	1808:1843	This research would be helpful for development of plant-derived cancer edible vaccines.
35697846	9	96	contain	containing	1280:1289	arg1	plants					1273:1278	rGA733-FcOX transgenic tomato plants	1243:1278	rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein	1243:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	9	96	contain	containing	1280:1289	arg2	FW					1314:1315	approximately 270 ng/g FW	1291:1315	approximately 270 ng/g FW of rGA733-Fc protein	1291:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	2	97	theme	strong	306:311	arg1	candidate					313:321	a strong candidate	304:321	a strong candidate to produce a colorectal cancer vaccine	304:360	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	2	97	theme	strong	306:311	arg1	GA733-2					211:217	GA733-2	211:217	GA733-2	211:217	GA733-2, the protein that is also known as colorectal carcinoma (CRC)-associated antigen, is a strong candidate to produce a colorectal cancer vaccine.
35697846	9	98	theme	protein	1330:1336	arg1	FW					1314:1315	approximately 270 ng/g FW	1291:1315	approximately 270 ng/g FW of rGA733-Fc protein	1291:1336	The red fruits of rGA733-FcOX transgenic tomato plants containing approximately 270 ng/g FW of rGA733-Fc protein were orally administered to C57BL/6 mice.
35697846	8	99	theme	glycosylation	1031:1043	arg1	analysis					1053:1060	Further glycosylation pattern analysis	1023:1060	Further glycosylation pattern analysis	1023:1060	Further glycosylation pattern analysis revealed that plant derived rGA733-Fc protein contains an oligomannose glycan structure, which is a typical glycosylation pattern found on ER-processing proteins.
35697846	10	100	theme	cancer	1482:1487	arg1	growth					1489:1494	colorectal cancer growth	1471:1494	colorectal cancer growth	1471:1494	Oral administration of tomato fruits of the rGA733-Fc expressing transgenic plants delayed colorectal cancer growth and stimulated immune responses compared to oral administration of tomato fruits of the h-Fc expressing transgenic plants in the C57BL/6J mice.
35697846	4	101	theme	immune	549:554	arg1	response					556:563	immune response	549:563	immune response	549:563	It also contains high content of vitamins that aid activation of immune response.
35409419	4	0	theme	glycosylation	839:851	arg1	sites					853:857	putative N-linked glycosylation sites	821:857	putative N-linked glycosylation sites	821:857	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	4	0	theme	glycosylation	839:851	arg1	alanine					797:803	alanine	797:803	alanine	797:803	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	2	1	from	glycosylation	385:397	arg1	HD					406:407	the HD	402:407	the HD	402:407	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	3	2	theme	transmembrane	652:664	arg1	TMD					674:676	TMD	674:676	TMD	674:676	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	2	theme	transmembrane	652:664	arg1	domain					666:671	transmembrane domain	652:671	transmembrane domain (TMD)	652:677	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	1	3	theme	CAR	235:237	arg1	design					239:244	enhanced CAR design	226:244	enhanced CAR design	226:244	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	9	4	theme	future	1669:1674	arg1	design					1680:1685	future CAR design	1669:1685	future CAR design	1669:1685	Our findings demonstrate that post-translational modifications of the CAR HD influence CAR-T cell activity, establishing a basis for future CAR design.
35409419	3	5	theme	vascular	512:519	arg1	VEGFR2					559:564	VEGFR2	559:564	VEGFR2	559:564	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	5	theme	vascular	512:519	arg1	receptor					547:554	a mouse vascular endothelial growth factor receptor 2	504:556	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)	504:565	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	7	6	link	O-linked	1320:1327	arg1	glycosylation					1329:1341	considerable O-linked glycosylation	1307:1341	considerable O-linked glycosylation	1307:1341	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	6	7	theme	antigen-specific	1137:1152	arg1	production					1163:1172	antigen-specific cytokine production	1137:1172	antigen-specific cytokine production	1137:1172	The absence of disulphide bonds did not affect membrane CAR expression but reduced antigen-specific cytokine production and cytotoxic activity.
35409419	1	8	theme	chimeric	155:162	arg1	CAR					182:184	CAR	182:184	CAR	182:184	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	1	8	theme	chimeric	155:162	arg1	receptor					172:179	chimeric antigen receptor	155:179	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	4	9	theme	putative	821:828	arg1	sites					853:857	putative N-linked glycosylation sites	821:857	putative N-linked glycosylation sites	821:857	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	4	9	theme	putative	821:828	arg1	alanine					797:803	alanine	797:803	alanine	797:803	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	3	10	link	CD3ζ-derived	685:696	arg1	domain					718:723	a CD3ζ-derived signal transduction domain	683:723	a CD3ζ-derived signal transduction domain (STD)	683:729	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	10	link	CD3ζ-derived	685:696	arg1	STD					726:728	STD	726:728	STD	726:728	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	1	11	theme	receptor	172:179	arg1	therapeutics					205:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	2	12	from	effects	330:336	arg1	function					418:425	CAR-T function	412:425	CAR-T function	412:425	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	2	12	from	effects	330:336	arg1	HD					406:407	the HD	402:407	the HD	402:407	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	2	12	from	effects	330:336	arg1	residues					372:379	cysteine residues	363:379	cysteine residues	363:379	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	6	13	theme	CAR	1110:1112	arg1	expression					1114:1123	membrane CAR expression	1101:1123	membrane CAR expression	1101:1123	The absence of disulphide bonds did not affect membrane CAR expression but reduced antigen-specific cytokine production and cytotoxic activity.
35409419	4	14	link	N-linked	830:837	arg1	sites					853:857	putative N-linked glycosylation sites	821:857	putative N-linked glycosylation sites	821:857	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	4	14	link	N-linked	830:837	arg1	alanine					797:803	alanine	797:803	alanine	797:803	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	3	15	theme	signal	698:703	arg1	domain					718:723	a CD3ζ-derived signal transduction domain	683:723	a CD3ζ-derived signal transduction domain (STD)	683:729	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	15	theme	signal	698:703	arg1	STD					726:728	STD	726:728	STD	726:728	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	16	theme	growth	533:538	arg1	VEGFR2					559:564	VEGFR2	559:564	VEGFR2	559:564	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	16	theme	growth	533:538	arg1	receptor					547:554	a mouse vascular endothelial growth factor receptor 2	504:556	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)	504:565	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	5	17	theme	residues	1044:1051	arg1	TMD-proximal					1011:1022	the more TMD-proximal	1002:1022	the more TMD-proximal of the two cysteine residues	1002:1051	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] formed homodimers, the former through a single HD cysteine residue and the latter through the more TMD-proximal of the two cysteine residues.
35409419	1	18	theme	-expressing	186:196	arg1	therapeutics					205:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	3	19	attach	linked	616:621	arg2	fragment					598:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment	504:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD	504:649	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	19	attach	linked	616:621	arg1	CD28-					626:630	CD28-	626:630	CD28-	626:630	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	19	attach	linked	616:621	arg1	HD					648:649	CD8α-derived HD	635:649	CD8α-derived HD	635:649	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	5	20	theme	more	1006:1009	arg1	TMD-proximal					1011:1022	the more TMD-proximal	1002:1022	the more TMD-proximal of the two cysteine residues	1002:1051	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] formed homodimers, the former through a single HD cysteine residue and the latter through the more TMD-proximal of the two cysteine residues.
35409419	4	21	with	alanine	797:803	arg1	aspartate					865:873	aspartate	865:873	aspartate	865:873	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	6	22	theme	cytotoxic	1178:1186	arg1	activity					1188:1195	cytotoxic activity	1178:1195	cytotoxic activity	1178:1195	The absence of disulphide bonds did not affect membrane CAR expression but reduced antigen-specific cytokine production and cytotoxic activity.
35409419	1	23	theme	cell	300:303	arg1	function					305:312	CAR-T cell function	294:312	CAR-T cell function	294:312	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	3	24	theme	single-chain	576:587	arg1	fragment					598:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment	504:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD	504:649	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	8	25	theme	CAR-T	1496:1500	arg1	activity					1507:1514	CAR-T cell activity	1496:1514	CAR-T cell activity	1496:1514	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	2	26	gly	glycosylation	385:397	arg1	HD					406:407	the HD	402:407	the HD	402:407	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	2	26	gly	glycosylation	385:397	arg1	residues					372:379	cysteine residues	363:379	cysteine residues	363:379	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	2	26	gly	glycosylation	385:397	arg2	residues					372:379	cysteine residues	363:379	cysteine residues	363:379	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	0	27	theme	Domain	53:58	arg1	Modifications					79:91	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	4	28	with	asparagine	809:818	arg1	aspartate					865:873	aspartate	865:873	aspartate	865:873	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	0	29	theme	Modifications	79:91	arg1	Effects					4:10	The Effects	0:10	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity	0:114	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	8	30	theme	CAR[V/8a/8a/3z	1519:1532	arg1	effect					1468:1473	no effect	1465:1473	no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z]	1465:1533	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	2	31	theme	cysteine	363:370	arg1	residues					372:379	cysteine residues	363:379	cysteine residues	363:379	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	0	32	theme	CAR-T	96:100	arg1	Activity					107:114	CAR-T Cell Activity	96:114	CAR-T Cell Activity	96:114	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	5	33	theme	single	952:957	arg1	residue					971:977	a single HD cysteine residue	950:977	a single HD cysteine residue	950:977	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] formed homodimers, the former through a single HD cysteine residue and the latter through the more TMD-proximal of the two cysteine residues.
35409419	2	34	theme	CAR-T	412:416	arg1	function					418:425	CAR-T function	412:425	CAR-T function	412:425	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	8	35	from	effect	1468:1473	arg1	activity					1507:1514	CAR-T cell activity	1496:1514	CAR-T cell activity	1496:1514	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	8	35	from	effect	1468:1473	arg1	expression					1482:1491	the expression	1478:1491	the expression	1478:1491	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	8	36	theme	N-linked	1369:1376	arg1	glycosylation					1378:1390	N-linked glycosylation	1369:1390	N-linked glycosylation of CAR[V/28/28/3z]	1369:1409	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	2	37	theme	bonding	352:358	arg1	effects					330:336	the effects	326:336	the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function	326:425	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	7	38	theme	unknown	1349:1355	arg1	site					1357:1360	an unknown site	1346:1360	an unknown site	1346:1360	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	3	39	used	used	431:434	arg2	We					428:429	We	428:429	We	428:429	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	1	40	theme	CAR	259:261	arg1	factors					274:280	CAR structural factors	259:280	CAR structural factors that affect CAR-T cell function	259:312	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	9	41	theme	CAR	1606:1608	arg1	HD					1610:1611	the CAR HD	1602:1611	the CAR HD	1602:1611	Our findings demonstrate that post-translational modifications of the CAR HD influence CAR-T cell activity, establishing a basis for future CAR design.
35409419	7	42	theme	considerable	1307:1318	arg1	glycosylation					1329:1341	considerable O-linked glycosylation	1307:1341	considerable O-linked glycosylation	1307:1341	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	0	43	theme	Antigen	24:30	arg1	Receptor					32:39	Chimeric Antigen Receptor	15:39	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	0	43	theme	Antigen	24:30	arg1	CAR					42:44	CAR	42:44	CAR	42:44	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	2	44	from	bonding	352:358	arg1	HD					406:407	the HD	402:407	the HD	402:407	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	8	45	theme	stable	1420:1425	arg1	expression					1440:1449	stable membrane CAR expression	1420:1449	stable membrane CAR expression	1420:1449	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	8	46	gly	glycosylation	1378:1390	arg1	CAR[V/28/28/3z					1395:1408	CAR[V/28/28/3z]	1395:1409	CAR[V/28/28/3z]	1395:1409	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	2	47	from	HD	406:407	arg1	effects					330:336	the effects	326:336	the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function	326:425	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	9	48	theme	cell	1629:1632	arg1	activity					1634:1641	CAR-T cell activity	1623:1641	CAR-T cell activity	1623:1641	Our findings demonstrate that post-translational modifications of the CAR HD influence CAR-T cell activity, establishing a basis for future CAR design.
35409419	4	49	theme	structural	747:756	arg1	variants					758:765	structural variants	747:765	structural variants	747:765	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	3	50	theme	first-generation	436:451	arg1	CAR[V/28/28/3z					453:466	first-generation CAR[V/28/28/3z]	436:467	first-generation CAR[V/28/28/3z]	436:467	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	1	51	theme	T	198:198	arg1	therapeutics					205:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	7	52	gly	glycosylation	1257:1269	arg2	site					1271:1274	one N-linked glycosylation site	1244:1274	one N-linked glycosylation site	1244:1274	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	7	52	gly	glycosylation	1257:1269	arg2	one					1244:1246	one	1244:1246	one	1244:1246	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	5	53	theme	cysteine	962:969	arg1	residue					971:977	a single HD cysteine residue	950:977	a single HD cysteine residue	950:977	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] formed homodimers, the former through a single HD cysteine residue and the latter through the more TMD-proximal of the two cysteine residues.
35409419	6	54	theme	bonds	1080:1084	arg1	absence					1058:1064	The absence	1054:1064	The absence of disulphide bonds	1054:1084	The absence of disulphide bonds did not affect membrane CAR expression but reduced antigen-specific cytokine production and cytotoxic activity.
35409419	3	55	theme	CD8α-derived	635:646	arg1	HD					648:649	CD8α-derived HD	635:649	CD8α-derived HD	635:649	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	4	56	gly	glycosylation	839:851	arg2	alanine					797:803	alanine	797:803	alanine	797:803	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	4	56	gly	glycosylation	839:851	arg2	sites					853:857	putative N-linked glycosylation sites	821:857	putative N-linked glycosylation sites	821:857	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	1	57	theme	therapeutics	205:216	arg1	efficacy					132:139	efficacy	132:139	efficacy	132:139	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	1	57	theme	therapeutics	205:216	arg1	safety					145:150	safety	145:150	safety	145:150	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	7	58	theme	N-linked	1248:1255	arg1	site					1271:1274	one N-linked glycosylation site	1244:1274	one N-linked glycosylation site	1244:1274	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	1	59	theme	enhanced	226:233	arg1	design					239:244	enhanced CAR design	226:244	enhanced CAR design	226:244	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	4	60	theme	N-linked	830:837	arg1	sites					853:857	putative N-linked glycosylation sites	821:857	putative N-linked glycosylation sites	821:857	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	4	60	theme	N-linked	830:837	arg1	alanine					797:803	alanine	797:803	alanine	797:803	We constructed structural variants by substituting cysteine with alanine and asparagine (putative N-linked glycosylation sites) with aspartate.
35409419	3	61	theme	mouse	506:510	arg1	VEGFR2					559:564	VEGFR2	559:564	VEGFR2	559:564	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	61	theme	mouse	506:510	arg1	receptor					547:554	a mouse vascular endothelial growth factor receptor 2	504:556	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)	504:565	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	9	62	theme	CAR	1676:1678	arg1	design					1680:1685	future CAR design	1669:1685	future CAR design	1669:1685	Our findings demonstrate that post-translational modifications of the CAR HD influence CAR-T cell activity, establishing a basis for future CAR design.
35409419	6	63	theme	cytokine	1154:1161	arg1	production					1163:1172	antigen-specific cytokine production	1137:1172	antigen-specific cytokine production	1137:1172	The absence of disulphide bonds did not affect membrane CAR expression but reduced antigen-specific cytokine production and cytotoxic activity.
35409419	3	64	theme	CD3ζ-derived	685:696	arg1	domain					718:723	a CD3ζ-derived signal transduction domain	683:723	a CD3ζ-derived signal transduction domain (STD)	683:729	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	64	theme	CD3ζ-derived	685:696	arg1	STD					726:728	STD	726:728	STD	726:728	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	65	theme	factor	540:545	arg1	VEGFR2					559:564	VEGFR2	559:564	VEGFR2	559:564	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	65	theme	factor	540:545	arg1	receptor					547:554	a mouse vascular endothelial growth factor receptor 2	504:556	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)	504:565	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	1	66	theme	antigen	164:170	arg1	CAR					182:184	CAR	182:184	CAR	182:184	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	1	66	theme	antigen	164:170	arg1	receptor					172:179	chimeric antigen receptor	155:179	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	3	67	theme	endothelial	521:531	arg1	VEGFR2					559:564	VEGFR2	559:564	VEGFR2	559:564	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	67	theme	endothelial	521:531	arg1	receptor					547:554	a mouse vascular endothelial growth factor receptor 2	504:556	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)	504:565	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	6	68	theme	membrane	1101:1108	arg1	expression					1114:1123	membrane CAR expression	1101:1123	membrane CAR expression	1101:1123	The absence of disulphide bonds did not affect membrane CAR expression but reduced antigen-specific cytokine production and cytotoxic activity.
35409419	3	69	theme	transduction	705:716	arg1	domain					718:723	a CD3ζ-derived signal transduction domain	683:723	a CD3ζ-derived signal transduction domain (STD)	683:729	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	3	69	theme	transduction	705:716	arg1	STD					726:728	STD	726:728	STD	726:728	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	5	70	theme	cysteine	1035:1042	arg1	residues					1044:1051	the two cysteine residues	1027:1051	the two cysteine residues	1027:1051	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] formed homodimers, the former through a single HD cysteine residue and the latter through the more TMD-proximal of the two cysteine residues.
35409419	7	71	link	N-linked	1248:1255	arg1	site					1271:1274	one N-linked glycosylation site	1244:1274	one N-linked glycosylation site	1244:1274	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	8	72	link	N-linked	1369:1376	arg1	glycosylation					1378:1390	N-linked glycosylation	1369:1390	N-linked glycosylation of CAR[V/28/28/3z]	1369:1409	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	8	73	theme	CAR	1436:1438	arg1	expression					1440:1449	stable membrane CAR expression	1420:1449	stable membrane CAR expression	1420:1449	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	3	74	theme	variable	589:596	arg1	fragment					598:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment	504:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD	504:649	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	0	75	theme	Hinge	47:51	arg1	Domain					53:58	Chimeric Antigen Receptor (CAR) Hinge Domain	15:58	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	3	76	theme	-specific	566:574	arg1	fragment					598:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment	504:605	a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD	504:649	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	8	77	theme	cell	1502:1505	arg1	activity					1507:1514	CAR-T cell activity	1496:1514	CAR-T cell activity	1496:1514	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	0	78	theme	Post-Translational	60:77	arg1	Modifications					79:91	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	0	79	from	Effects	4:10	arg1	Activity					107:114	CAR-T Cell Activity	96:114	CAR-T Cell Activity	96:114	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	0	80	theme	Cell	102:105	arg1	Activity					107:114	CAR-T Cell Activity	96:114	CAR-T Cell Activity	96:114	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	7	81	gly	glycosylation	1329:1341	arg1	CAR[V/8a/8a/3z					1281:1294	CAR[V/8a/8a/3z]	1281:1295	CAR[V/8a/8a/3z]	1281:1295	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	7	81	gly	glycosylation	1329:1341	arg1	site					1357:1360	an unknown site	1346:1360	an unknown site	1346:1360	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	7	82	theme	O-linked	1320:1327	arg1	glycosylation					1329:1341	considerable O-linked glycosylation	1307:1341	considerable O-linked glycosylation	1307:1341	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	2	83	theme	disulphide	341:350	arg1	bonding					352:358	disulphide bonding	341:358	disulphide bonding at cysteine residues	341:379	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	0	84	theme	Chimeric	15:22	arg1	Receptor					32:39	Chimeric Antigen Receptor	15:39	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	0	84	theme	Chimeric	15:22	arg1	CAR					42:44	CAR	42:44	CAR	42:44	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	1	85	dep	efficacy	132:139	arg1	the					128:130	the	128:130	the	128:130	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	2	86	theme	glycosylation	385:397	arg1	effects					330:336	the effects	326:336	the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function	326:425	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	0	87	theme	Receptor	32:39	arg1	Domain					53:58	Chimeric Antigen Receptor (CAR) Hinge Domain	15:58	Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications	15:91	The Effects of Chimeric Antigen Receptor (CAR) Hinge Domain Post-Translational Modifications on CAR-T Cell Activity.
35409419	1	88	theme	structural	263:272	arg1	factors					274:280	CAR structural factors	259:280	CAR structural factors that affect CAR-T cell function	259:312	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	9	89	theme	HD	1610:1611	arg1	modifications					1585:1597	post-translational modifications	1566:1597	post-translational modifications of the CAR HD	1566:1611	Our findings demonstrate that post-translational modifications of the CAR HD influence CAR-T cell activity, establishing a basis for future CAR design.
35409419	8	90	theme	CAR[V/28/28/3z	1395:1408	arg1	glycosylation					1378:1390	N-linked glycosylation	1369:1390	N-linked glycosylation of CAR[V/28/28/3z]	1369:1409	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	9	91	theme	CAR-T	1623:1627	arg1	activity					1634:1641	CAR-T cell activity	1623:1641	CAR-T cell activity	1623:1641	Our findings demonstrate that post-translational modifications of the CAR HD influence CAR-T cell activity, establishing a basis for future CAR design.
35409419	1	92	theme	CAR-T	294:298	arg1	function					305:312	CAR-T cell function	294:312	CAR-T cell function	294:312	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	5	93	theme	HD	959:960	arg1	residue					971:977	a single HD cysteine residue	950:977	a single HD cysteine residue	950:977	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] formed homodimers, the former through a single HD cysteine residue and the latter through the more TMD-proximal of the two cysteine residues.
35409419	7	94	theme	glycosylation	1257:1269	arg1	site					1271:1274	one N-linked glycosylation site	1244:1274	one N-linked glycosylation site	1244:1274	CAR[V/28/28/3z] and CAR[V/8a/8a/3z] harboured one N-linked glycosylation site, and CAR[V/8a/8a/3z] underwent considerable O-linked glycosylation at an unknown site.
35409419	3	95	link	CD8α-derived	635:646	arg1	HD					648:649	CD8α-derived HD	635:649	CD8α-derived HD	635:649	We used first-generation CAR[V/28/28/3z] and CAR[V/8a/8a/3z], consisting of a mouse vascular endothelial growth factor receptor 2 (VEGFR2)-specific single-chain variable fragment tandemly linked to CD28- or CD8α-derived HD, transmembrane domain (TMD) and a CD3ζ-derived signal transduction domain (STD).
35409419	9	96	theme	post-translational	1566:1583	arg1	modifications					1585:1597	post-translational modifications	1566:1597	post-translational modifications of the CAR HD	1566:1611	Our findings demonstrate that post-translational modifications of the CAR HD influence CAR-T cell activity, establishing a basis for future CAR design.
35409419	8	97	theme	membrane	1427:1434	arg1	expression					1440:1449	stable membrane CAR expression	1420:1449	stable membrane CAR expression	1420:1449	Thus, N-linked glycosylation of CAR[V/28/28/3z] promotes stable membrane CAR expression, while having no effect on the expression or CAR-T cell activity of CAR[V/8a/8a/3z].
35409419	1	98	theme	cell	200:203	arg1	therapeutics					205:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	chimeric antigen receptor (CAR)-expressing T cell therapeutics	155:216	To improve the efficacy and safety of chimeric antigen receptor (CAR)-expressing T cell therapeutics through enhanced CAR design, we analysed CAR structural factors that affect CAR-T cell function.
35409419	2	99	from	residues	372:379	arg1	glycosylation					385:397	glycosylation	385:397	glycosylation in the HD	385:407	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	2	99	from	residues	372:379	arg1	effects					330:336	the effects	326:336	the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function	326:425	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	2	99	from	residues	372:379	arg1	bonding					352:358	disulphide bonding	341:358	disulphide bonding at cysteine residues	341:379	We studied the effects of disulphide bonding at cysteine residues and glycosylation in the HD on CAR-T function.
35409419	6	100	theme	disulphide	1069:1078	arg1	bonds					1080:1084	disulphide bonds	1069:1084	disulphide bonds	1069:1084	The absence of disulphide bonds did not affect membrane CAR expression but reduced antigen-specific cytokine production and cytotoxic activity.
37373433	1	0	theme	kombucha	325:332	arg1	fungus					338:343	kombucha tea fungus	325:343	kombucha tea fungus	325:343	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	3	1	theme	antioxidant	501:511	arg1	activity					513:520	The antioxidant activity	497:520	The antioxidant activity of the tested samples	497:542	The antioxidant activity of the tested samples was assessed using DPPH and ABTS assays.
37373433	5	2	theme	anti-aging	750:759	arg1	properties					761:770	Potential anti-aging properties	740:770	Potential anti-aging properties	740:770	Potential anti-aging properties were determined by their ability to inhibit the activity of the metalloproteinases collagenase and elastase.
37373433	0	3	theme	Sambucus	78:85	arg1	Fruit					93:97	Sambucus nigra Fruit	78:97	Sambucus nigra Fruit	78:97	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	1	4	theme	tea	334:336	arg1	fungus					338:343	kombucha tea fungus	325:343	kombucha tea fungus	325:343	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	7	5	from	cells	1265:1269	arg1	levels					1103:1108	levels	1103:1108	levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells	1103:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	6	theme	lipopolysaccharide	1221:1238	arg1	cells					1265:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	1	7	theme	Sambucus	250:257	arg1	extract					271:277	the Sambucus nigra fruit extract	246:277	the Sambucus nigra fruit extract	246:277	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	8	8	theme	cell	1401:1404	arg1	damage					1406:1411	free-radical-induced cell damage	1380:1411	free-radical-induced cell damage	1380:1411	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	7	9	from	levels	1103:1108	arg1	cells					1265:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	0	10	theme	Human	114:118	arg1	Fibroblasts					125:135	LPS-Induced Human Skin Fibroblasts	102:135	LPS-Induced Human Skin Fibroblasts	102:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	4	11	theme	cells	733:737	arg1	metabolism					686:695	metabolism	686:695	metabolism	686:695	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	4	11	theme	cells	733:737	arg1	viability					672:680	viability	672:680	viability	672:680	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	7	12	theme	pro-inflammatory	1117:1132	arg1	factor					1175:1180	tumor necrosis factor	1160:1180	tumor necrosis factor (TNF-α)	1160:1188	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	12	theme	pro-inflammatory	1117:1132	arg1	IL-10					1212:1216	anti-inflammatory IL-10	1194:1216	anti-inflammatory IL-10	1194:1216	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	12	theme	pro-inflammatory	1117:1132	arg1	interleukins					1134:1145	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10	1113:1216	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells	1113:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	12	theme	pro-inflammatory	1117:1132	arg1	IL-1ß					1153:1157	IL-1ß	1153:1157	IL-1ß	1153:1157	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	12	theme	pro-inflammatory	1117:1132	arg1	IL-6					1147:1150	IL-6	1147:1150	IL-6	1147:1150	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	8	13	theme	S.	1307:1308	arg1	extract					1316:1322	the S. nigra extract	1303:1322	the S. nigra extract	1303:1322	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	8	13	theme	S.	1307:1308	arg1	effective					1356:1364	effective	1356:1364	effective	1356:1364	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	3	14	theme	tested	529:534	arg1	samples					536:542	the tested samples	525:542	the tested samples	525:542	The antioxidant activity of the tested samples was assessed using DPPH and ABTS assays.
37373433	8	15	from	effects	1431:1437	arg1	health					1452:1457	skin cell health	1442:1457	skin cell health	1442:1457	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	2	16	theme	non-fermented	378:390	arg1	extracts					392:399	fermented and non-fermented extracts	364:399	fermented and non-fermented extracts	364:399	For this purpose, fermented and non-fermented extracts were compared in terms of their chemical composition by the HPLC/ESI-MS chromatographic method.
37373433	0	17	theme	LPS-Induced	102:112	arg1	Fibroblasts					125:135	LPS-Induced Human Skin Fibroblasts	102:135	LPS-Induced Human Skin Fibroblasts	102:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	0	18	theme	Water	44:48	arg1	Extract					50:56	Water Extract	44:56	Water Extract	44:56	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	3	19	theme	samples	536:542	arg1	activity					513:520	The antioxidant activity	497:520	The antioxidant activity of the tested samples	497:542	The antioxidant activity of the tested samples was assessed using DPPH and ABTS assays.
37373433	5	20	theme	Potential	740:748	arg1	properties					761:770	Potential anti-aging properties	740:770	Potential anti-aging properties	740:770	Potential anti-aging properties were determined by their ability to inhibit the activity of the metalloproteinases collagenase and elastase.
37373433	2	21	theme	chemical	433:440	arg1	composition					442:452	their chemical composition	427:452	their chemical composition	427:452	For this purpose, fermented and non-fermented extracts were compared in terms of their chemical composition by the HPLC/ESI-MS chromatographic method.
37373433	2	22	theme	chromatographic	473:487	arg1	method					489:494	the HPLC/ESI-MS chromatographic method	457:494	the HPLC/ESI-MS chromatographic method	457:494	For this purpose, fermented and non-fermented extracts were compared in terms of their chemical composition by the HPLC/ESI-MS chromatographic method.
37373433	6	23	theme	types	1000:1004	arg1	proliferation					973:985	the proliferation	969:985	the proliferation of both cell types	969:1004	Tests showed that the extract and the ferment have antioxidant properties and stimulate the proliferation of both cell types.
37373433	8	24	contain	have	1417:1420	arg1	extract					1316:1322	the S. nigra extract	1303:1322	the S. nigra extract	1303:1322	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	8	24	contain	have	1417:1420	arg1	effective					1356:1364	effective	1356:1364	effective	1356:1364	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	8	24	contain	have	1417:1420	arg2	effects					1431:1437	positive effects	1422:1437	positive effects on skin cell health	1422:1457	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	2	25	theme	fermented	364:372	arg1	extracts					392:399	fermented and non-fermented extracts	364:399	fermented and non-fermented extracts	364:399	For this purpose, fermented and non-fermented extracts were compared in terms of their chemical composition by the HPLC/ESI-MS chromatographic method.
37373433	0	26	theme	Anti-Inflammatory	0:16	arg1	Effects					33:39	Anti-Inflammatory and Protective Effects	0:39	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts	0:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	1	27	theme	fruit	265:269	arg1	extract					271:277	the Sambucus nigra fruit extract	246:277	the Sambucus nigra fruit extract	246:277	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	0	28	dep	Sambucus	78:85	arg1	nigra					87:91	nigra	87:91	nigra	87:91	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	1	29	theme	extract	271:277	arg1	effects					235:241	the antioxidant, anti-inflammatory and protective effects	185:241	the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract	185:277	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	5	30	theme	metalloproteinases	836:853	arg1	activity					820:827	the activity	816:827	the activity of the metalloproteinases collagenase and elastase	816:878	Potential anti-aging properties were determined by their ability to inhibit the activity of the metalloproteinases collagenase and elastase.
37373433	0	31	theme	Protective	22:31	arg1	Effects					33:39	Anti-Inflammatory and Protective Effects	0:39	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts	0:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	0	32	theme	Skin	120:123	arg1	Fibroblasts					125:135	LPS-Induced Human Skin Fibroblasts	102:135	LPS-Induced Human Skin Fibroblasts	102:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	7	33	dep	interleukins	1134:1145	arg1	IL-1ß					1153:1157	IL-1ß	1153:1157	IL-1ß	1153:1157	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	33	dep	interleukins	1134:1145	arg1	TNF-α					1183:1187	TNF-α	1183:1187	TNF-α	1183:1187	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	33	dep	interleukins	1134:1145	arg1	interleukins					1134:1145	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10	1113:1216	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells	1113:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	33	dep	interleukins	1134:1145	arg1	factor					1175:1180	tumor necrosis factor	1160:1180	tumor necrosis factor (TNF-α)	1160:1188	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	33	dep	interleukins	1134:1145	arg1	IL-10					1212:1216	anti-inflammatory IL-10	1194:1216	anti-inflammatory IL-10	1194:1216	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	33	dep	interleukins	1134:1145	arg1	IL-6					1147:1150	IL-6	1147:1150	IL-6	1147:1150	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	3	34	theme	DPPH	563:566	arg1	assays					577:582	DPPH and ABTS assays	563:582	DPPH and ABTS assays	563:582	The antioxidant activity of the tested samples was assessed using DPPH and ABTS assays.
37373433	6	35	theme	antioxidant	932:942	arg1	properties					944:953	antioxidant properties	932:953	antioxidant properties	932:953	Tests showed that the extract and the ferment have antioxidant properties and stimulate the proliferation of both cell types.
37373433	4	36	theme	Blue	631:634	arg1	tests					652:656	Alamar Blue and Neutral Red tests	624:656	Alamar Blue and Neutral Red tests	624:656	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	2	37	theme	HPLC/ESI-MS	461:471	arg1	method					489:494	the HPLC/ESI-MS chromatographic method	457:494	the HPLC/ESI-MS chromatographic method	457:494	For this purpose, fermented and non-fermented extracts were compared in terms of their chemical composition by the HPLC/ESI-MS chromatographic method.
37373433	7	38	from	interleukins	1134:1145	arg1	cells					1265:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	4	39	theme	Red	648:650	arg1	tests					652:656	Alamar Blue and Neutral Red tests	624:656	Alamar Blue and Neutral Red tests	624:656	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	8	40	theme	positive	1422:1429	arg1	effects					1431:1437	positive effects	1422:1437	positive effects on skin cell health	1422:1457	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	1	41	theme	antioxidant	189:199	arg1	effects					235:241	the antioxidant, anti-inflammatory and protective effects	185:241	the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract	185:277	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	4	42	theme	Alamar	624:629	arg1	tests					652:656	Alamar Blue and Neutral Red tests	624:656	Alamar Blue and Neutral Red tests	624:656	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	7	43	theme	anti-inflammatory	1035:1051	arg1	activity					1053:1060	the anti-inflammatory activity	1031:1060	the anti-inflammatory activity of the extract	1031:1075	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	4	44	theme	Neutral	640:646	arg1	tests					652:656	Alamar Blue and Neutral Red tests	624:656	Alamar Blue and Neutral Red tests	624:656	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	7	45	theme	tumor	1160:1164	arg1	factor					1175:1180	tumor necrosis factor	1160:1180	tumor necrosis factor (TNF-α)	1160:1188	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	45	theme	tumor	1160:1164	arg1	interleukins					1134:1145	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10	1113:1216	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells	1113:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	45	theme	tumor	1160:1164	arg1	TNF-α					1183:1187	TNF-α	1183:1187	TNF-α	1183:1187	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	46	theme	extract	1069:1075	arg1	activity					1053:1060	the anti-inflammatory activity	1031:1060	the anti-inflammatory activity of the extract	1031:1075	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	6	47	theme	cell	995:998	arg1	types					1000:1004	both cell types	990:1004	both cell types	990:1004	Tests showed that the extract and the ferment have antioxidant properties and stimulate the proliferation of both cell types.
37373433	8	48	dep	kombucha	1332:1339	arg1	ferment					1341:1347	ferment	1341:1347	ferment	1341:1347	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	8	49	theme	free-radical-induced	1380:1399	arg1	damage					1406:1411	free-radical-induced cell damage	1380:1411	free-radical-induced cell damage	1380:1411	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	0	50	theme	Extract	50:56	arg1	Effects					33:39	Anti-Inflammatory and Protective Effects	0:39	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts	0:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	7	51	theme	-treated	1245:1252	arg1	cells					1265:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	1	52	dep	Sambucus	250:257	arg1	nigra					259:263	nigra	259:263	nigra	259:263	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	7	53	theme	interleukins	1134:1145	arg1	levels					1103:1108	levels	1103:1108	levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells	1103:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	4	54	theme	fibroblast	700:709	arg1	metabolism					686:695	metabolism	686:695	metabolism	686:695	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	4	54	theme	fibroblast	700:709	arg1	viability					672:680	viability	672:680	viability	672:680	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	8	55	theme	cell	1447:1450	arg1	health					1452:1457	skin cell health	1442:1457	skin cell health	1442:1457	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	7	56	theme	necrosis	1166:1173	arg1	factor					1175:1180	tumor necrosis factor	1160:1180	tumor necrosis factor (TNF-α)	1160:1188	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	56	theme	necrosis	1166:1173	arg1	interleukins					1134:1145	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10	1113:1216	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells	1113:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	56	theme	necrosis	1166:1173	arg1	TNF-α					1183:1187	TNF-α	1183:1187	TNF-α	1183:1187	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	4	57	theme	skin	728:731	arg1	cells					733:737	keratinocyte skin cells	715:737	keratinocyte skin cells	715:737	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	1	58	theme	anti-inflammatory	202:218	arg1	effects					235:241	the antioxidant, anti-inflammatory and protective effects	185:241	the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract	185:277	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	4	59	dep	viability	672:680	arg1	the					668:670	the	668:670	the	668:670	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	8	60	theme	skin	1442:1445	arg1	health					1452:1457	skin cell health	1442:1457	skin cell health	1442:1457	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	4	61	theme	keratinocyte	715:726	arg1	cells					733:737	keratinocyte skin cells	715:737	keratinocyte skin cells	715:737	Cytotoxicity was also determined using Alamar Blue and Neutral Red tests to assess the viability and metabolism of fibroblast and keratinocyte skin cells.
37373433	2	62	theme	composition	442:452	arg1	terms					418:422	terms	418:422	terms of their chemical composition	418:452	For this purpose, fermented and non-fermented extracts were compared in terms of their chemical composition by the HPLC/ESI-MS chromatographic method.
37373433	7	63	theme	fibroblast	1254:1263	arg1	cells					1265:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	lipopolysaccharide (LPS)-treated fibroblast cells	1221:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	0	64	from	Fruit	93:97	arg1	Bioferment					62:71	Bioferment	62:71	Bioferment	62:71	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	0	64	from	Fruit	93:97	arg1	Effects					33:39	Anti-Inflammatory and Protective Effects	0:39	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts	0:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	0	64	from	Fruit	93:97	arg1	Extract					50:56	Water Extract	44:56	Water Extract	44:56	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	5	65	dep	metalloproteinases	836:853	arg1	metalloproteinases					836:853	the metalloproteinases collagenase and elastase	832:878	the metalloproteinases collagenase and elastase	832:878	Potential anti-aging properties were determined by their ability to inhibit the activity of the metalloproteinases collagenase and elastase.
37373433	5	65	dep	metalloproteinases	836:853	arg1	elastase					871:878	elastase	871:878	elastase	871:878	Potential anti-aging properties were determined by their ability to inhibit the activity of the metalloproteinases collagenase and elastase.
37373433	5	65	dep	metalloproteinases	836:853	arg1	collagenase					855:865	collagenase	855:865	collagenase	855:865	Potential anti-aging properties were determined by their ability to inhibit the activity of the metalloproteinases collagenase and elastase.
37373433	6	66	contain	have	927:930	arg2	properties					944:953	antioxidant properties	932:953	antioxidant properties	932:953	Tests showed that the extract and the ferment have antioxidant properties and stimulate the proliferation of both cell types.
37373433	6	66	contain	have	927:930	arg1	extract					903:909	the extract	899:909	the extract	899:909	Tests showed that the extract and the ferment have antioxidant properties and stimulate the proliferation of both cell types.
37373433	6	66	contain	have	927:930	arg1	ferment					919:925	ferment	919:925	ferment	919:925	Tests showed that the extract and the ferment have antioxidant properties and stimulate the proliferation of both cell types.
37373433	1	67	theme	protective	224:233	arg1	effects					235:241	the antioxidant, anti-inflammatory and protective effects	185:241	the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract	185:277	In this study, an attempt was made to evaluate the antioxidant, anti-inflammatory and protective effects of the Sambucus nigra fruit extract and its ferment obtained by fermentation with kombucha tea fungus.
37373433	0	68	theme	Bioferment	62:71	arg1	Effects					33:39	Anti-Inflammatory and Protective Effects	0:39	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts	0:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	0	69	from	Effects	33:39	arg1	Fibroblasts					125:135	LPS-Induced Human Skin Fibroblasts	102:135	LPS-Induced Human Skin Fibroblasts	102:135	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	0	69	from	Effects	33:39	arg1	Fruit					93:97	Sambucus nigra Fruit	78:97	Sambucus nigra Fruit	78:97	Anti-Inflammatory and Protective Effects of Water Extract and Bioferment from Sambucus nigra Fruit in LPS-Induced Human Skin Fibroblasts.
37373433	8	70	dep	S.	1307:1308	arg1	nigra					1310:1314	nigra	1310:1314	nigra	1310:1314	The results indicate that both the S. nigra extract and its kombucha ferment can be effective in preventing free-radical-induced cell damage and have positive effects on skin cell health.
37373433	3	71	theme	ABTS	572:575	arg1	assays					577:582	DPPH and ABTS assays	563:582	DPPH and ABTS assays	563:582	The antioxidant activity of the tested samples was assessed using DPPH and ABTS assays.
37373433	7	72	theme	anti-inflammatory	1194:1210	arg1	IL-10					1212:1216	anti-inflammatory IL-10	1194:1216	anti-inflammatory IL-10	1194:1216	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
37373433	7	72	theme	anti-inflammatory	1194:1210	arg1	interleukins					1134:1145	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10	1113:1216	the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells	1113:1269	The study also assessed the anti-inflammatory activity of the extract and ferment by monitoring levels of the pro-inflammatory interleukins IL-6, IL-1ß, tumor necrosis factor (TNF-α) and anti-inflammatory IL-10 in lipopolysaccharide (LPS)-treated fibroblast cells.
35055112	6	0	theme	early	927:931	arg1	signaling					940:948	early TGF-β1 signaling	927:948	early TGF-β1 signaling	927:948	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	1	1	theme	chondrogenic	156:167	arg1	development					169:179	chondrogenic development	156:179	chondrogenic development	156:179	In 3D bioprinting for cartilage regeneration, bioinks that support chondrogenic development are of key importance.
35055112	4	2	theme	covalent	552:559	arg1	tethering					561:569	covalent tethering	552:569	covalent tethering of transforming growth factor-beta 1 (TGF-β1)	552:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	5	3	theme	mesenchymal	638:648	arg1	MSCs					665:668	MSCs	665:668	MSCs	665:668	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	5	3	theme	mesenchymal	638:648	arg1	cells					658:662	mesenchymal stromal cells	638:662	Bone marrow-derived mesenchymal stromal cells (MSCs)	618:669	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	8	4	theme	factor	1416:1421	arg1	supply					1423:1428	continuous exogenous growth factor supply	1388:1428	continuous exogenous growth factor supply	1388:1428	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	6	5	theme	construct	1057:1065	arg1	stiffness					1067:1075	resulting construct stiffness	1047:1075	resulting construct stiffness	1047:1075	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	3	6	theme	factors	389:395	arg1	functionality					356:368	the functionality	352:368	the functionality of tethered growth factors within 3D printable bioinks	352:423	However, studies that investigate the functionality of tethered growth factors within 3D printable bioinks are still lacking.
35055112	6	7	theme	chondrogenic	951:962	arg1	expression					969:978	chondrogenic gene expression	951:978	chondrogenic gene expression	951:978	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	7	8	theme	bioinks	1171:1177	arg1	analysis					1142:1149	a comparative analysis	1128:1149	a comparative analysis of cast and printed bioinks	1128:1177	Furthermore, it was successfully demonstrated, in a comparative analysis of cast and printed bioinks, that covalently tethered TGF-β1 maintained its functionality after 3D printing.
35055112	5	9	theme	stromal	650:656	arg1	MSCs					665:668	MSCs	665:668	MSCs	665:668	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	5	9	theme	stromal	650:656	arg1	cells					658:662	mesenchymal stromal cells	638:662	Bone marrow-derived mesenchymal stromal cells (MSCs)	618:669	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	6	10	theme	resulting	1047:1055	arg1	stiffness					1067:1075	resulting construct stiffness	1047:1075	resulting construct stiffness	1047:1075	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	4	11	theme	crosslinked	498:508	arg1	bioink					532:537	a dual-stage crosslinked hyaluronic acid-based bioink	485:537	a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1)	485:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	3	12	theme	tethered	373:380	arg1	factors					389:395	tethered growth factors	373:395	tethered growth factors	373:395	However, studies that investigate the functionality of tethered growth factors within 3D printable bioinks are still lacking.
35055112	4	13	theme	dual-stage	487:496	arg1	bioink					532:537	a dual-stage crosslinked hyaluronic acid-based bioink	485:537	a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1)	485:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	8	14	theme	high-quality	1332:1343	arg1	tissues					1359:1365	high-quality cartilaginous tissues	1332:1365	high-quality cartilaginous tissues	1332:1365	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	9	15	theme	other	1650:1654	arg1	applications					1671:1682	other biofabrication applications	1650:1682	other biofabrication applications	1650:1682	Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.
35055112	9	16	theme	growth	1533:1538	arg1	factor					1540:1545	growth factor	1533:1545	growth factor tethering within bioinks, potentially leading to superior tissue development,	1533:1623	Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.
35055112	3	17	theme	growth	382:387	arg1	factors					389:395	tethered growth factors	373:395	tethered growth factors	373:395	However, studies that investigate the functionality of tethered growth factors within 3D printable bioinks are still lacking.
35055112	4	18	theme	acid-based	521:530	arg1	bioink					532:537	a dual-stage crosslinked hyaluronic acid-based bioink	485:537	a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1)	485:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	7	19	theme	printed	1163:1169	arg1	bioinks					1171:1177	cast and printed bioinks	1154:1177	cast and printed bioinks	1154:1177	Furthermore, it was successfully demonstrated, in a comparative analysis of cast and printed bioinks, that covalently tethered TGF-β1 maintained its functionality after 3D printing.
35055112	9	20	theme	biofabrication	1656:1669	arg1	applications					1671:1682	other biofabrication applications	1650:1682	other biofabrication applications	1650:1682	Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.
35055112	4	21	link	crosslinked	498:508	arg1	bioink					532:537	a dual-stage crosslinked hyaluronic acid-based bioink	485:537	a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1)	485:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	7	22	theme	3D	1247:1248	arg1	printing					1250:1257	3D printing	1247:1257	3D printing	1247:1257	Furthermore, it was successfully demonstrated, in a comparative analysis of cast and printed bioinks, that covalently tethered TGF-β1 maintained its functionality after 3D printing.
35055112	4	23	theme	hyaluronic	510:519	arg1	bioink					532:537	a dual-stage crosslinked hyaluronic acid-based bioink	485:537	a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1)	485:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	8	24	theme	great	1447:1451	arg1	potential					1453:1461	great potential	1447:1461	great potential for future investigation towards cartilage regeneration	1447:1517	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	1	25	theme	key	188:190	arg1	importance					192:201	key importance	188:201	key importance	188:201	In 3D bioprinting for cartilage regeneration, bioinks that support chondrogenic development are of key importance.
35055112	6	26	theme	gene	964:967	arg1	expression					969:978	chondrogenic gene expression	951:978	chondrogenic gene expression	951:978	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	6	27	theme	qualitative	981:991	arg1	deposition					1014:1023	qualitative and quantitative ECM deposition	981:1023	qualitative and quantitative ECM deposition	981:1023	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	1	28	theme	3D	92:93	arg1	bioprinting					95:105	3D bioprinting	92:105	3D bioprinting for cartilage regeneration	92:132	In 3D bioprinting for cartilage regeneration, bioinks that support chondrogenic development are of key importance.
35055112	3	29	theme	3D	404:405	arg1	bioinks					417:423	3D printable bioinks	404:423	3D printable bioinks	404:423	However, studies that investigate the functionality of tethered growth factors within 3D printable bioinks are still lacking.
35055112	4	30	theme	factor-beta	594:604	arg1	tethering					561:569	covalent tethering	552:569	covalent tethering of transforming growth factor-beta 1 (TGF-β1)	552:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	7	31	theme	comparative	1130:1140	arg1	analysis					1142:1149	a comparative analysis	1128:1149	a comparative analysis of cast and printed bioinks	1128:1177	Furthermore, it was successfully demonstrated, in a comparative analysis of cast and printed bioinks, that covalently tethered TGF-β1 maintained its functionality after 3D printing.
35055112	5	32	theme	marrow-derived	623:636	arg1	MSCs					665:668	MSCs	665:668	MSCs	665:668	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	5	32	theme	marrow-derived	623:636	arg1	cells					658:662	mesenchymal stromal cells	638:662	Bone marrow-derived mesenchymal stromal cells (MSCs)	618:669	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	5	33	with	constructs	837:846	arg1	TGF-β1					881:886	non-covalently incorporated TGF-β1	853:886	non-covalently incorporated TGF-β1	853:886	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	8	34	theme	cartilaginous	1345:1357	arg1	tissues					1359:1365	high-quality cartilaginous tissues	1332:1365	high-quality cartilaginous tissues	1332:1365	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	3	35	theme	printable	407:415	arg1	bioinks					417:423	3D printable bioinks	404:423	3D printable bioinks	404:423	However, studies that investigate the functionality of tethered growth factors within 3D printable bioinks are still lacking.
35055112	9	36	theme	tissue	1605:1610	arg1	development					1612:1622	superior tissue development	1596:1622	superior tissue development	1596:1622	Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.
35055112	8	37	dep	enabled	1306:1312	arg1	bears					1441:1445	bears	1441:1445	bears great potential for future investigation towards cartilage regeneration	1441:1517	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	7	38	theme	cast	1154:1157	arg1	bioinks					1171:1177	cast and printed bioinks	1154:1177	cast and printed bioinks	1154:1177	Furthermore, it was successfully demonstrated, in a comparative analysis of cast and printed bioinks, that covalently tethered TGF-β1 maintained its functionality after 3D printing.
35055112	0	39	theme	Acid-Based	32:41	arg1	Bioink					43:48	a Hyaluronic Acid-Based Bioink	19:48	a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues	19:86	Tethered TGF-β1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues.
35055112	9	40	theme	superior	1596:1603	arg1	development					1612:1622	superior tissue development	1596:1622	superior tissue development	1596:1622	Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.
35055112	5	41	theme	MSCs	748:751	arg1	differentiation					729:743	chondrogenic differentiation	716:743	chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1	716:797	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	0	42	theme	Hyaluronic	21:30	arg1	Bioink					43:48	a Hyaluronic Acid-Based Bioink	19:48	a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues	19:86	Tethered TGF-β1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues.
35055112	6	43	theme	ECM	1010:1012	arg1	deposition					1014:1023	qualitative and quantitative ECM deposition	981:1023	qualitative and quantitative ECM deposition	981:1023	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	2	44	theme	non-printable	239:251	arg1	hydrogels					253:261	non-printable hydrogels	239:261	non-printable hydrogels	239:261	Growth factors covalently bound in non-printable hydrogels have been shown to effectively promote chondrogenesis.
35055112	5	45	theme	tethered	783:790	arg1	TGF-β1					792:797	tethered TGF-β1	783:797	tethered TGF-β1	783:797	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	7	46	theme	tethered	1196:1203	arg1	TGF-β1					1205:1210	covalently tethered TGF-β1	1185:1210	covalently tethered TGF-β1	1185:1210	Furthermore, it was successfully demonstrated, in a comparative analysis of cast and printed bioinks, that covalently tethered TGF-β1 maintained its functionality after 3D printing.
35055112	5	47	theme	bioink	760:765	arg1	constructs					767:776	bioink constructs	760:776	bioink constructs with tethered TGF-β1	760:797	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	8	48	theme	cartilage	1496:1504	arg1	regeneration					1506:1517	cartilage regeneration	1496:1517	cartilage regeneration	1496:1517	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	6	49	theme	TGF-β1	933:938	arg1	signaling					940:948	early TGF-β1 signaling	927:948	early TGF-β1 signaling	927:948	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35055112	1	50	theme	cartilage	111:119	arg1	regeneration					121:132	cartilage regeneration	111:132	cartilage regeneration	111:132	In 3D bioprinting for cartilage regeneration, bioinks that support chondrogenic development are of key importance.
35055112	8	51	theme	ink	1290:1292	arg1	composition					1294:1304	the presented ink composition	1276:1304	the presented ink composition	1276:1304	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	4	52	theme	growth	587:592	arg1	factor-beta					594:604	transforming growth factor-beta 1	574:606	transforming growth factor-beta 1 (TGF-β1)	574:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	4	52	theme	growth	587:592	arg1	TGF-β1					609:614	TGF-β1	609:614	TGF-β1	609:614	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	5	53	with	constructs	767:776	arg1	TGF-β1					792:797	tethered TGF-β1	783:797	tethered TGF-β1	783:797	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	8	54	theme	presented	1280:1288	arg1	composition					1294:1304	the presented ink composition	1276:1304	the presented ink composition	1276:1304	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	4	55	theme	transforming	574:585	arg1	factor-beta					594:604	transforming growth factor-beta 1	574:606	transforming growth factor-beta 1 (TGF-β1)	574:615	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	4	55	theme	transforming	574:585	arg1	TGF-β1					609:614	TGF-β1	609:614	TGF-β1	609:614	Therefore, in this study, we established a dual-stage crosslinked hyaluronic acid-based bioink that enabled covalent tethering of transforming growth factor-beta 1 (TGF-β1).
35055112	8	56	theme	future	1467:1472	arg1	investigation					1474:1486	future investigation	1467:1486	future investigation	1467:1486	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	8	57	theme	continuous	1388:1397	arg1	supply					1423:1428	continuous exogenous growth factor supply	1388:1428	continuous exogenous growth factor supply	1388:1428	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	5	58	theme	chondrogenic	716:727	arg1	differentiation					729:743	chondrogenic differentiation	716:743	chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1	716:797	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	2	59	theme	Growth	204:209	arg1	factors					211:217	Growth factors	204:217	Growth factors covalently bound in non-printable hydrogels	204:261	Growth factors covalently bound in non-printable hydrogels have been shown to effectively promote chondrogenesis.
35055112	5	60	link	marrow-derived	623:636	arg1	MSCs					665:668	MSCs	665:668	MSCs	665:668	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	5	60	link	marrow-derived	623:636	arg1	cells					658:662	mesenchymal stromal cells	638:662	Bone marrow-derived mesenchymal stromal cells (MSCs)	618:669	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	0	61	theme	Cartilaginous	66:78	arg1	Tissues					80:86	Bioprinting Cartilaginous Tissues	54:86	Bioprinting Cartilaginous Tissues	54:86	Tethered TGF-β1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues.
35055112	8	62	theme	tissues	1359:1365	arg1	generation					1318:1327	the generation	1314:1327	the generation of high-quality cartilaginous tissues	1314:1365	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	9	63	dep	bioinks	1564:1570	arg1	leading					1585:1591	leading	1585:1591	leading to superior tissue development	1585:1622	Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.
35055112	1	64	from	importance	192:201	arg1	bioprinting					95:105	3D bioprinting	92:105	3D bioprinting for cartilage regeneration	92:132	In 3D bioprinting for cartilage regeneration, bioinks that support chondrogenic development are of key importance.
35055112	8	65	theme	growth	1409:1414	arg1	supply					1423:1428	continuous exogenous growth factor supply	1388:1428	continuous exogenous growth factor supply	1388:1428	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	9	66	theme	factor	1540:1545	arg1	tethering					1547:1555	growth factor tethering	1533:1555	growth factor tethering within bioinks, potentially leading to superior tissue development,	1533:1623	Furthermore, growth factor tethering within bioinks, potentially leading to superior tissue development, may also be explored for other biofabrication applications.
35055112	0	67	theme	Bioprinting	54:64	arg1	Tissues					80:86	Bioprinting Cartilaginous Tissues	54:86	Bioprinting Cartilaginous Tissues	54:86	Tethered TGF-β1 in a Hyaluronic Acid-Based Bioink for Bioprinting Cartilaginous Tissues.
35055112	5	68	theme	incorporated	868:879	arg1	TGF-β1					881:886	non-covalently incorporated TGF-β1	853:886	non-covalently incorporated TGF-β1	853:886	Bone marrow-derived mesenchymal stromal cells (MSCs) were cultured over three weeks in vitro, and chondrogenic differentiation of MSCs within bioink constructs with tethered TGF-β1 was markedly enhanced, as compared to constructs with non-covalently incorporated TGF-β1.
35055112	8	69	theme	exogenous	1399:1407	arg1	supply					1423:1428	continuous exogenous growth factor supply	1388:1428	continuous exogenous growth factor supply	1388:1428	Taken together, the presented ink composition enabled the generation of high-quality cartilaginous tissues without the need for continuous exogenous growth factor supply and, thus, bears great potential for future investigation towards cartilage regeneration.
35055112	6	70	theme	quantitative	997:1008	arg1	deposition					1014:1023	qualitative and quantitative ECM deposition	981:1023	qualitative and quantitative ECM deposition	981:1023	This was substantiated with regard to early TGF-β1 signaling, chondrogenic gene expression, qualitative and quantitative ECM deposition and distribution, and resulting construct stiffness.
35592296	5	0	dep	phagocytosis	745:756	arg1	the					741:743	the	741:743	the	741:743	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	6	1	theme	glucuronic	966:975	arg1	acid					977:980	glucuronic acid	966:980	glucuronic acid	966:980	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	7	2	theme	new	1180:1182	arg1	BJP					1106:1108	BJP	1106:1108	BJP	1106:1108	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	7	2	theme	new	1180:1182	arg1	foods					1195:1199	new functional foods	1180:1199	new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements	1180:1301	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	7	2	theme	new	1180:1182	arg1	polysaccharides					1114:1128	polysaccharides	1114:1128	polysaccharides	1114:1128	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	4	3	dep	had	584:586	arg1	beneficial					637:646	beneficial	637:646	beneficial	637:646	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	6	4	theme	molecular	931:939	arg1	weights					941:947	their molecular weights	925:947	their molecular weights	925:947	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	5	5	theme	obvious	802:808	arg1	cytotoxicity					810:821	obvious cytotoxicity	802:821	obvious cytotoxicity	802:821	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	6	6	theme	different	1058:1066	arg1	cultivars					1068:1076	different cultivars	1058:1076	different cultivars	1058:1076	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	0	7	from	characteristics	16:30	arg1	cultivars					93:101	five cultivars	88:101	five cultivars of Chrysanthemi Flos	88:122	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	5	8	theme	Immunoregulatory	691:706	arg1	assay					708:712	Immunoregulatory assay	691:712	Immunoregulatory assay	691:712	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	4	9	theme	surface	607:613	arg1	structures					615:624	looser and uniform surface structures	588:624	looser and uniform surface structures	588:624	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	5	10	theme	RAW264.7	779:786	arg1	cells					788:792	RAW264.7 cells	779:792	RAW264.7 cells	779:792	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	0	11	theme	Flos	119:122	arg1	cultivars					93:101	five cultivars	88:101	five cultivars of Chrysanthemi Flos	88:122	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	1	12	dep	characteristics	165:179	arg1	the					145:147	the	145:147	the	145:147	This study compared the physicochemical characteristics and immunomodulatory activities of chrysanthemums' polysaccharides (JPs) from five cultivars.
35592296	3	13	contain	had	519:521	arg2	polysaccharides					489:503	polysaccharides	489:503	polysaccharides	489:503	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	3	13	contain	had	519:521	arg1	Polysaccharides					409:423	Polysaccharides	409:423	Polysaccharides of Gongju (GJP)	409:439	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	3	13	contain	had	519:521	arg2	populations					473:483	the lowest molecular weight populations	445:483	the lowest molecular weight populations	445:483	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	3	13	contain	had	519:521	arg2	highest					527:533	highest	527:533	highest	527:533	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	5	14	theme	NO	879:880	arg1	level					852:856	the release level	840:856	the release level of TNF-α, IFN-γ, and NO	840:880	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	0	15	theme	polysaccharides	67:81	arg1	characteristics					16:30	Physicochemical characteristics	0:30	Physicochemical characteristics	0:30	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	0	15	theme	polysaccharides	67:81	arg1	activities					53:62	immunoregulatory activities	36:62	immunoregulatory activities	36:62	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	2	16	theme	molecular	317:325	arg1	weights					327:333	the molecular weights	313:333	the molecular weights	313:333	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	6	17	theme	arabinose	986:994	arg1	microstructure					1001:1014	microstructure	1001:1014	microstructure	1001:1014	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	6	17	theme	arabinose	986:994	arg1	weights					941:947	their molecular weights	925:947	their molecular weights	925:947	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	6	17	theme	arabinose	986:994	arg1	contents					954:961	the contents	950:961	the contents of glucuronic acid and arabinose	950:994	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	5	18	theme	TNF-α	861:865	arg1	level					852:856	the release level	840:856	the release level of TNF-α, IFN-γ, and NO	840:880	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	0	19	from	activities	53:62	arg1	cultivars					93:101	five cultivars	88:101	five cultivars of Chrysanthemi Flos	88:122	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	2	20	theme	compositions	365:376	arg1	properties					397:406	morphological properties	383:406	morphological properties	383:406	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	2	20	theme	compositions	365:376	arg1	weights					327:333	the molecular weights	313:333	the molecular weights	313:333	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	2	20	theme	compositions	365:376	arg1	ratios					340:345	the ratios	336:345	the ratios of monosaccharide compositions	336:376	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	0	21	theme	Physicochemical	0:14	arg1	characteristics					16:30	Physicochemical characteristics	0:30	Physicochemical characteristics	0:30	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	4	22	theme	looser	588:593	arg1	structures					615:624	looser and uniform surface structures	588:624	looser and uniform surface structures	588:624	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	2	23	theme	monosaccharide	350:363	arg1	compositions					365:376	monosaccharide compositions	350:376	monosaccharide compositions	350:376	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	7	24	theme	functional	1184:1193	arg1	BJP					1106:1108	BJP	1106:1108	BJP	1106:1108	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	7	24	theme	functional	1184:1193	arg1	foods					1195:1199	new functional foods	1180:1199	new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements	1180:1301	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	7	24	theme	functional	1184:1193	arg1	polysaccharides					1114:1128	polysaccharides	1114:1128	polysaccharides	1114:1128	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	0	25	theme	immunoregulatory	36:51	arg1	activities					53:62	immunoregulatory activities	36:62	immunoregulatory activities	36:62	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	7	26	theme	Hangbaiju	1133:1141	arg1	BJP					1106:1108	BJP	1106:1108	BJP	1106:1108	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	7	26	theme	Hangbaiju	1133:1141	arg1	foods					1195:1199	new functional foods	1180:1199	new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements	1180:1301	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	7	26	theme	Hangbaiju	1133:1141	arg1	polysaccharides					1114:1128	polysaccharides	1114:1128	polysaccharides	1114:1128	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	4	27	contain	had	584:586	arg1	polysaccharides					560:574	polysaccharides	560:574	polysaccharides	560:574	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	4	27	contain	had	584:586	arg2	structures					615:624	looser and uniform surface structures	588:624	looser and uniform surface structures	588:624	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	4	27	contain	had	584:586	arg1	GJP					552:554	GJP	552:554	GJP	552:554	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	3	28	theme	BJP	514:516	arg1	polysaccharides					489:503	polysaccharides	489:503	polysaccharides	489:503	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	3	28	theme	BJP	514:516	arg1	populations					473:483	the lowest molecular weight populations	445:483	the lowest molecular weight populations	445:483	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	3	29	theme	GJP	436:438	arg1	Polysaccharides					409:423	Polysaccharides	409:423	Polysaccharides of Gongju (GJP)	409:439	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	4	30	theme	uniform	599:605	arg1	structures					615:624	looser and uniform surface structures	588:624	looser and uniform surface structures	588:624	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	5	31	theme	IFN-γ	868:872	arg1	level					852:856	the release level	840:856	the release level of TNF-α, IFN-γ, and NO	840:880	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	3	32	theme	molecular	456:464	arg1	populations					473:483	the lowest molecular weight populations	445:483	the lowest molecular weight populations	445:483	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	3	33	theme	weight	466:471	arg1	populations					473:483	the lowest molecular weight populations	445:483	the lowest molecular weight populations	445:483	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	5	34	theme	release	844:850	arg1	level					852:856	the release level	840:856	the release level of TNF-α, IFN-γ, and NO	840:880	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	0	35	from	cultivars	93:101	arg1	polysaccharides					67:81	polysaccharides	67:81	polysaccharides from five cultivars of Chrysanthemi Flos	67:122	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	0	35	from	cultivars	93:101	arg1	characteristics					16:30	Physicochemical characteristics	0:30	Physicochemical characteristics	0:30	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	0	35	from	cultivars	93:101	arg1	activities					53:62	immunoregulatory activities	36:62	immunoregulatory activities	36:62	Physicochemical characteristics and immunoregulatory activities of polysaccharides from five cultivars of Chrysanthemi Flos.
35592296	2	36	theme	Significant	275:285	arg1	differences					287:297	Significant differences	275:297	Significant differences	275:297	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	4	37	theme	instant	673:679	arg1	products					681:688	instant products	673:688	instant products	673:688	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	3	38	theme	lowest	449:454	arg1	populations					473:483	the lowest molecular weight populations	445:483	the lowest molecular weight populations	445:483	Polysaccharides of Gongju (GJP) had the lowest molecular weight populations and polysaccharides of Boju (BJP) had the highest.
35592296	4	39	theme	Qiju	579:582	arg1	polysaccharides					560:574	polysaccharides	560:574	polysaccharides	560:574	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	4	39	theme	Qiju	579:582	arg1	GJP					552:554	GJP	552:554	GJP	552:554	SEM showed that GJP and polysaccharides of Qiju had looser and uniform surface structures, which are beneficial for being developed into instant products.
35592296	6	40	theme	Immune-enhancing	883:898	arg1	activity					900:907	Immune-enhancing activity	883:907	Immune-enhancing activity	883:907	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	2	41	theme	morphological	383:395	arg1	properties					397:406	morphological properties	383:406	morphological properties	383:406	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	1	42	from	cultivars	264:272	arg1	polysaccharides					232:246	chrysanthemums' polysaccharides	216:246	chrysanthemums' polysaccharides (JPs) from five cultivars	216:272	This study compared the physicochemical characteristics and immunomodulatory activities of chrysanthemums' polysaccharides (JPs) from five cultivars.
35592296	1	42	from	cultivars	264:272	arg1	JPs					249:251	JPs	249:251	JPs	249:251	This study compared the physicochemical characteristics and immunomodulatory activities of chrysanthemums' polysaccharides (JPs) from five cultivars.
35592296	1	43	theme	physicochemical	149:163	arg1	characteristics					165:179	physicochemical characteristics	149:179	physicochemical characteristics	149:179	This study compared the physicochemical characteristics and immunomodulatory activities of chrysanthemums' polysaccharides (JPs) from five cultivars.
35592296	5	44	theme	cells	788:792	arg1	proliferation					762:774	proliferation	762:774	proliferation	762:774	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	5	44	theme	cells	788:792	arg1	phagocytosis					745:756	phagocytosis	745:756	phagocytosis	745:756	Immunoregulatory assay revealed that JPs enhanced the phagocytosis and proliferation of RAW264.7 cells without obvious cytotoxicity, and upregulated the release level of TNF-α, IFN-γ, and NO.
35592296	7	45	theme	immunization	1277:1288	arg1	requirements					1290:1301	the immunization requirements	1273:1301	the immunization requirements	1273:1301	The results suggested that BJP and polysaccharides of Hangbaiju are more suitable to be developed as new functional foods for enhancing immunity, and provided a reference for selection based on the immunization requirements.
35592296	6	46	theme	acid	977:980	arg1	microstructure					1001:1014	microstructure	1001:1014	microstructure	1001:1014	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	6	46	theme	acid	977:980	arg1	weights					941:947	their molecular weights	925:947	their molecular weights	925:947	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	6	46	theme	acid	977:980	arg1	contents					954:961	the contents	950:961	the contents of glucuronic acid and arabinose	950:994	Immune-enhancing activity correlated with their molecular weights, the contents of glucuronic acid and arabinose, and microstructure, which performed differently according to different cultivars.
35592296	1	47	theme	immunomodulatory	185:200	arg1	activities					202:211	immunomodulatory activities	185:211	immunomodulatory activities	185:211	This study compared the physicochemical characteristics and immunomodulatory activities of chrysanthemums' polysaccharides (JPs) from five cultivars.
35592296	2	48	located	found	304:308	arg1	properties					397:406	morphological properties	383:406	morphological properties	383:406	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	2	48	located	found	304:308	arg2	differences					287:297	Significant differences	275:297	Significant differences	275:297	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	2	48	located	found	304:308	arg1	weights					327:333	the molecular weights	313:333	the molecular weights	313:333	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
35592296	2	48	located	found	304:308	arg1	ratios					340:345	the ratios	336:345	the ratios of monosaccharide compositions	336:376	Significant differences were found in the molecular weights, the ratios of monosaccharide compositions, and morphological properties.
37074187	7	0	theme	reduced	1117:1123	arg1	activity					1130:1137	reduced CanB activity	1117:1137	reduced CanB activity	1117:1137	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	10	1	theme	failures	1809:1816	arg1	majority					1787:1794	the majority	1783:1794	the majority of treatment failures	1783:1816	IMPORTANCE Acinetobacter baumannii has become resistant to all classes of antibiotics, with β-lactam resistance responsible for the majority of treatment failures.
37074187	13	2	theme	new	2100:2102	arg1	method					2104:2109	a new method	2098:2109	a new method for treating A. baumannii infections	2098:2146	Inhibitors of carbonic anhydrase could represent a new method for treating A. baumannii infections.
37074187	2	3	theme	altered	408:414	arg1	activity					435:442	altered l,d-transpeptidase activity	408:442	activity	435:442	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	2	4	theme	composition	289:299	arg1	analysis					301:308	peptidoglycan composition analysis	275:308	peptidoglycan composition analysis	275:308	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	1	5	theme	Acinetobacter	168:180	arg1	chromosome					192:201	the Acinetobacter baumannii chromosome	164:201	the Acinetobacter baumannii chromosome	164:201	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	13	6	theme	anhydrase	2072:2080	arg1	Inhibitors					2049:2058	Inhibitors	2049:2058	Inhibitors of carbonic anhydrase	2049:2080	Inhibitors of carbonic anhydrase could represent a new method for treating A. baumannii infections.
37074187	9	7	theme	anhydrase	1500:1508	arg1	CanB					1510:1513	the essential carbonic anhydrase CanB	1477:1513	the essential carbonic anhydrase CanB	1477:1513	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	5	8	theme	viability	825:833	arg1	loss					817:820	a more pronounced loss	799:820	a more pronounced loss of viability	799:833	A canB deletion mutant exhibited a more pronounced loss of viability than the transposon insertion, and this became amplified when cells overexpressed ADC-7.
37074187	4	9	theme	significant	697:707	arg1	loss					709:712	a significant loss	695:712	a significant loss of viability	695:725	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	4	10	theme	canB	648:651	arg1	end					641:643	the distal 3' end	627:643	the distal 3' end of canB	627:651	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	5	11	theme	pronounced	806:815	arg1	loss					817:820	a more pronounced loss	799:820	a more pronounced loss of viability	799:833	A canB deletion mutant exhibited a more pronounced loss of viability than the transposon insertion, and this became amplified when cells overexpressed ADC-7.
37074187	1	12	contain	have	222:225	arg1	it					215:216	it	215:216	it	215:216	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	12	contain	have	222:225	arg2	function					259:266	a yet-to-be-identified cellular function	227:266	a yet-to-be-identified cellular function	227:266	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	13	theme	AmpC	120:123	arg1	β-lactamase					126:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	13	theme	AmpC	120:123	arg1	present					153:159	present	153:159	present	153:159	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	4	14	theme	viability	717:725	arg1	loss					709:712	a significant loss	695:712	a significant loss of viability	695:725	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	9	15	theme	antimicrobial	1548:1560	arg1	agents					1562:1567	antimicrobial agents	1548:1567	antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii	1548:1652	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	7	16	theme	inhibitor	1236:1244	arg1	ethoxzolamide					1246:1258	the carbonic anhydrase inhibitor ethoxzolamide	1213:1258	the carbonic anhydrase inhibitor ethoxzolamide	1213:1258	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	7	17	theme	carbonic	1217:1224	arg1	anhydrase					1226:1234	the carbonic anhydrase	1213:1234	the carbonic anhydrase inhibitor ethoxzolamide	1213:1258	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	1	18	from	present	153:159	arg1	chromosome					192:201	the Acinetobacter baumannii chromosome	164:201	the Acinetobacter baumannii chromosome	164:201	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	6	19	theme	β-lactamases	977:988	arg1	overexpression					939:952	overexpression	939:952	overexpression of the OXA-23 or TEM-1 β-lactamases	939:988	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	12	20	theme	reduced	1997:2003	arg1	activity					2024:2031	reduced carbonic anhydrase activity	1997:2031	reduced carbonic anhydrase activity	1997:2031	This study had uncovered a new genetic vulnerability in β-lactamase-expressing A. baumannii, where reduced carbonic anhydrase activity becomes lethal.
37074187	6	21	theme	anhydrase	1064:1072	arg1	activity					1074:1081	reduced carbonic anhydrase activity	1047:1081	reduced carbonic anhydrase activity	1047:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	10	22	theme	antibiotics	1729:1739	arg1	classes					1718:1724	all classes	1714:1724	all classes of antibiotics	1714:1739	IMPORTANCE Acinetobacter baumannii has become resistant to all classes of antibiotics, with β-lactam resistance responsible for the majority of treatment failures.
37074187	0	23	theme	Collateral	0:9	arg1	Changes					11:17	Collateral Changes	0:17	Collateral Changes in Cell Physiology	0:36	Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii.
37074187	12	24	theme	anhydrase	2014:2022	arg1	activity					2024:2031	reduced carbonic anhydrase activity	1997:2031	reduced carbonic anhydrase activity	1997:2031	This study had uncovered a new genetic vulnerability in β-lactamase-expressing A. baumannii, where reduced carbonic anhydrase activity becomes lethal.
37074187	13	25	dep	A.	2124:2125	arg1	baumannii					2127:2135	baumannii	2127:2135	baumannii	2127:2135	Inhibitors of carbonic anhydrase could represent a new method for treating A. baumannii infections.
37074187	6	26	theme	reduced	1047:1053	arg1	anhydrase					1064:1072	reduced carbonic anhydrase	1047:1072	reduced carbonic anhydrase activity	1047:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	1	27	theme	ADC	115:117	arg1	β-lactamase					126:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	27	theme	ADC	115:117	arg1	present					153:159	present	153:159	present	153:159	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	4	28	theme	transposon	575:584	arg1	insertions					586:595	transposon insertions	575:595	transposon insertions	575:595	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	7	29	theme	peptidoglycan	1171:1183	arg1	inhibitors					1195:1204	peptidoglycan synthesis inhibitors	1171:1204	peptidoglycan synthesis inhibitors	1171:1204	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	2	30	dep	activity	435:442	arg1	d-transpeptidase					418:433	altered l,d-transpeptidase activity	408:442	d-transpeptidase	418:433	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	5	31	theme	deletion	773:780	arg1	mutant					782:787	A canB deletion mutant	766:787	A canB deletion mutant	766:787	A canB deletion mutant exhibited a more pronounced loss of viability than the transposon insertion, and this became amplified when cells overexpressed ADC-7.
37074187	6	32	theme	viability	1023:1031	arg1	loss					1015:1018	a pronounced loss	1002:1018	a pronounced loss of viability in cells with reduced carbonic anhydrase activity	1002:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	0	33	theme	β-Lactamase	60:70	arg1	Expression					72:81	ADC-7 β-Lactamase Expression	54:81	ADC-7 β-Lactamase Expression in Acinetobacter baumannii	54:108	Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii.
37074187	10	34	theme	treatment	1799:1807	arg1	failures					1809:1816	treatment failures	1799:1816	treatment failures	1799:1816	IMPORTANCE Acinetobacter baumannii has become resistant to all classes of antibiotics, with β-lactam resistance responsible for the majority of treatment failures.
37074187	12	35	theme	genetic	1929:1935	arg1	vulnerability					1937:1949	a new genetic vulnerability	1923:1949	a new genetic vulnerability	1923:1949	This study had uncovered a new genetic vulnerability in β-lactamase-expressing A. baumannii, where reduced carbonic anhydrase activity becomes lethal.
37074187	12	36	theme	new	1925:1927	arg1	vulnerability					1937:1949	a new genetic vulnerability	1923:1949	a new genetic vulnerability	1923:1949	This study had uncovered a new genetic vulnerability in β-lactamase-expressing A. baumannii, where reduced carbonic anhydrase activity becomes lethal.
37074187	9	37	theme	ADC-7	1421:1425	arg1	overexpression					1427:1440	ADC-7 overexpression	1421:1440	ADC-7 overexpression	1421:1440	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	2	38	with	consistent	392:401	arg1	activity					435:442	altered l,d-transpeptidase activity	408:442	activity	435:442	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	2	39	theme	consistent	392:401	arg1	changes					384:390	changes	384:390	changes consistent with altered l,d-transpeptidase activity	384:442	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	9	40	theme	essential	1481:1489	arg1	anhydrase					1500:1508	the essential carbonic anhydrase	1477:1508	the essential carbonic anhydrase CanB	1477:1513	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	1	41	theme	yet-to-be-identified	229:248	arg1	function					259:266	a yet-to-be-identified cellular function	227:266	a yet-to-be-identified cellular function	227:266	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	0	42	theme	ADC-7	54:58	arg1	Expression					72:81	ADC-7 β-Lactamase Expression	54:81	ADC-7 β-Lactamase Expression in Acinetobacter baumannii	54:108	Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii.
37074187	4	43	theme	distal	631:636	arg1	end					641:643	the distal 3' end	627:643	the distal 3' end of canB	627:651	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	13	44	theme	carbonic	2063:2070	arg1	anhydrase					2072:2080	carbonic anhydrase	2063:2080	carbonic anhydrase	2063:2080	Inhibitors of carbonic anhydrase could represent a new method for treating A. baumannii infections.
37074187	9	45	theme	carbonic	1491:1498	arg1	anhydrase					1500:1508	the essential carbonic anhydrase	1477:1508	the essential carbonic anhydrase CanB	1477:1513	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	5	46	theme	transposon	844:853	arg1	insertion					855:863	the transposon insertion	840:863	the transposon insertion	840:863	A canB deletion mutant exhibited a more pronounced loss of viability than the transposon insertion, and this became amplified when cells overexpressed ADC-7.
37074187	1	47	attach	present	153:159	arg2	present					153:159	present	153:159	present	153:159	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	47	attach	present	153:159	arg1	chromosome					192:201	the Acinetobacter baumannii chromosome	164:201	the Acinetobacter baumannii chromosome	164:201	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	47	attach	present	153:159	arg2	β-lactamase					126:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	6	48	from	viability	1023:1031	arg1	cells					1036:1040	cells	1036:1040	cells with reduced carbonic anhydrase activity	1036:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	1	49	theme	baumannii	182:190	arg1	chromosome					192:201	the Acinetobacter baumannii chromosome	164:201	the Acinetobacter baumannii chromosome	164:201	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	6	50	theme	TEM-1	971:975	arg1	β-lactamases					977:988	the OXA-23 or TEM-1 β-lactamases	957:988	β-lactamases	977:988	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	4	51	theme	carbonic	663:670	arg1	anhydrase					672:680	carbonic anhydrase	663:680	carbonic anhydrase	663:680	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	6	52	from	cells	1036:1040	arg1	loss					1015:1018	a pronounced loss	1002:1018	a pronounced loss of viability in cells with reduced carbonic anhydrase activity	1002:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	6	53	theme	OXA-23	961:966	arg1	β-lactamases					977:988	the OXA-23 or TEM-1 β-lactamases	957:988	β-lactamases	977:988	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	10	54	dep	IMPORTANCE	1655:1664	arg1	baumannii					1680:1688	Acinetobacter baumannii	1666:1688	IMPORTANCE Acinetobacter baumannii	1655:1688	IMPORTANCE Acinetobacter baumannii has become resistant to all classes of antibiotics, with β-lactam resistance responsible for the majority of treatment failures.
37074187	0	55	from	Expression	72:81	arg1	baumannii					100:108	Acinetobacter baumannii	86:108	Acinetobacter baumannii	86:108	Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii.
37074187	9	56	theme	novel	1531:1535	arg1	target					1537:1542	a novel target	1529:1542	a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii	1529:1652	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	8	57	theme	synergistic	1298:1308	arg1	interaction					1310:1320	a synergistic interaction	1296:1320	a synergistic interaction with the peptidoglycan inhibitor fosfomycin and ethoxzolamide	1296:1382	Furthermore, this strain exhibited a synergistic interaction with the peptidoglycan inhibitor fosfomycin and ethoxzolamide.
37074187	7	58	theme	increased	1146:1154	arg1	sensitivity					1156:1166	increased sensitivity	1146:1166	increased sensitivity to peptidoglycan synthesis inhibitors	1146:1204	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	2	59	theme	peptidoglycan	275:287	arg1	analysis					301:308	peptidoglycan composition analysis	275:308	peptidoglycan composition analysis	275:308	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	7	60	theme	anhydrase	1226:1234	arg1	ethoxzolamide					1246:1258	the carbonic anhydrase inhibitor ethoxzolamide	1213:1258	the carbonic anhydrase inhibitor ethoxzolamide	1213:1258	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	1	61	from	chromosome	192:201	arg1	β-lactamase					126:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	61	from	chromosome	192:201	arg1	present					153:159	present	153:159	present	153:159	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	6	62	theme	pronounced	1004:1013	arg1	loss					1015:1018	a pronounced loss	1002:1018	a pronounced loss of viability in cells with reduced carbonic anhydrase activity	1002:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	8	63	theme	peptidoglycan	1331:1343	arg1	inhibitor					1345:1353	the peptidoglycan inhibitor fosfomycin and ethoxzolamide	1327:1382	inhibitor	1345:1353	Furthermore, this strain exhibited a synergistic interaction with the peptidoglycan inhibitor fosfomycin and ethoxzolamide.
37074187	11	64	theme	antimicrobials	1834:1847	arg1	classes					1823:1829	New classes	1819:1829	New classes of antimicrobials	1819:1847	New classes of antimicrobials are needed to treat this high-priority pathogen.
37074187	0	65	theme	Acinetobacter	86:98	arg1	baumannii					100:108	Acinetobacter baumannii	86:108	Acinetobacter baumannii	86:108	Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii.
37074187	9	66	from	impact	1411:1416	arg1	physiology					1450:1459	cell physiology	1445:1459	cell physiology	1445:1459	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	0	67	from	Changes	11:17	arg1	Physiology					27:36	Cell Physiology	22:36	Cell Physiology	22:36	Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii.
37074187	11	68	theme	high-priority	1874:1886	arg1	pathogen					1888:1895	this high-priority pathogen	1869:1895	this high-priority pathogen	1869:1895	New classes of antimicrobials are needed to treat this high-priority pathogen.
37074187	4	69	from	insertion	614:622	arg1	end					641:643	the distal 3' end	627:643	the distal 3' end of canB	627:651	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	9	70	theme	increased	1588:1596	arg1	potency					1598:1604	increased potency	1588:1604	increased potency against β-lactamase-overexpressing A. baumannii	1588:1652	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	6	71	from	loss	1015:1018	arg1	cells					1036:1040	cells	1036:1040	cells with reduced carbonic anhydrase activity	1036:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	7	72	theme	synthesis	1185:1193	arg1	inhibitors					1195:1204	peptidoglycan synthesis inhibitors	1171:1204	peptidoglycan synthesis inhibitors	1171:1204	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	12	73	theme	carbonic	2005:2012	arg1	anhydrase					2014:2022	carbonic anhydrase	2005:2022	reduced carbonic anhydrase activity	1997:2031	This study had uncovered a new genetic vulnerability in β-lactamase-expressing A. baumannii, where reduced carbonic anhydrase activity becomes lethal.
37074187	2	74	theme	l	416:416	arg1	activity					435:442	altered l,d-transpeptidase activity	408:442	activity	435:442	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	1	75	located	present	153:159	arg2	present					153:159	present	153:159	present	153:159	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	75	located	present	153:159	arg1	chromosome					192:201	the Acinetobacter baumannii chromosome	164:201	the Acinetobacter baumannii chromosome	164:201	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	1	75	located	present	153:159	arg2	β-lactamase					126:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase	111:136	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	6	76	theme	carbonic	1055:1062	arg1	anhydrase					1064:1072	reduced carbonic anhydrase	1047:1072	reduced carbonic anhydrase activity	1047:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	0	77	theme	Cell	22:25	arg1	Physiology					27:36	Cell Physiology	22:36	Cell Physiology	22:36	Collateral Changes in Cell Physiology Associated with ADC-7 β-Lactamase Expression in Acinetobacter baumannii.
37074187	4	78	theme	insertions	586:595	arg1	screen					565:570	a screen	563:570	a screen of transposon insertions	563:595	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	4	79	theme	adc-7	736:740	arg1	gene					742:745	the adc-7 gene	732:745	the adc-7 gene	732:745	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	10	80	theme	responsible	1767:1777	arg1	resistance					1756:1765	β-lactam resistance	1747:1765	β-lactam resistance responsible for the majority of treatment failures	1747:1816	IMPORTANCE Acinetobacter baumannii has become resistant to all classes of antibiotics, with β-lactam resistance responsible for the majority of treatment failures.
37074187	5	81	theme	canB	768:771	arg1	mutant					782:787	A canB deletion mutant	766:787	A canB deletion mutant	766:787	A canB deletion mutant exhibited a more pronounced loss of viability than the transposon insertion, and this became amplified when cells overexpressed ADC-7.
37074187	4	82	theme	principle	552:560	arg1	proof					543:547	proof	543:547	proof of principle	543:560	As proof of principle, a screen of transposon insertions revealed that an insertion in the distal 3' end of canB, encoding carbonic anhydrase, resulted in a significant loss of viability when the adc-7 gene was overexpressed.
37074187	8	83	with	interaction	1310:1320	arg1	inhibitor					1345:1353	the peptidoglycan inhibitor fosfomycin and ethoxzolamide	1327:1382	inhibitor	1345:1353	Furthermore, this strain exhibited a synergistic interaction with the peptidoglycan inhibitor fosfomycin and ethoxzolamide.
37074187	8	83	with	interaction	1310:1320	arg1	ethoxzolamide					1370:1382	ethoxzolamide	1370:1382	ethoxzolamide	1370:1382	Furthermore, this strain exhibited a synergistic interaction with the peptidoglycan inhibitor fosfomycin and ethoxzolamide.
37074187	10	84	theme	β-lactam	1747:1754	arg1	resistance					1756:1765	β-lactam resistance	1747:1765	β-lactam resistance responsible for the majority of treatment failures	1747:1816	IMPORTANCE Acinetobacter baumannii has become resistant to all classes of antibiotics, with β-lactam resistance responsible for the majority of treatment failures.
37074187	7	85	theme	CanB	1125:1128	arg1	activity					1130:1137	reduced CanB activity	1117:1137	reduced CanB activity	1117:1137	In addition, we demonstrate that reduced CanB activity led to increased sensitivity to peptidoglycan synthesis inhibitors and to the carbonic anhydrase inhibitor ethoxzolamide.
37074187	8	86	dep	inhibitor	1345:1353	arg1	fosfomycin					1355:1364	fosfomycin	1355:1364	fosfomycin	1355:1364	Furthermore, this strain exhibited a synergistic interaction with the peptidoglycan inhibitor fosfomycin and ethoxzolamide.
37074187	11	87	theme	New	1819:1821	arg1	classes					1823:1829	New classes	1819:1829	New classes of antimicrobials	1819:1847	New classes of antimicrobials are needed to treat this high-priority pathogen.
37074187	3	88	theme	new	519:521	arg1	vulnerabilities					523:537	new vulnerabilities	519:537	new vulnerabilities	519:537	Based on this, we tested whether cells overexpressing ADC-7 would exhibit new vulnerabilities.
37074187	2	89	theme	ADC-7	343:347	arg1	β-lactamase					349:359	the ADC-7 β-lactamase	339:359	the ADC-7 β-lactamase	339:359	Using peptidoglycan composition analysis, we show that overexpressing the ADC-7 β-lactamase in A. baumannii drives changes consistent with altered l,d-transpeptidase activity.
37074187	9	90	theme	overexpression	1427:1440	arg1	impact					1411:1416	the impact	1407:1416	the impact of ADC-7 overexpression on cell physiology	1407:1459	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
37074187	6	91	with	cells	1036:1040	arg1	activity					1074:1081	reduced carbonic anhydrase activity	1047:1081	reduced carbonic anhydrase activity	1047:1081	Interestingly, overexpression of the OXA-23 or TEM-1 β-lactamases also led to a pronounced loss of viability in cells with reduced carbonic anhydrase activity.
37074187	10	92	with	resistant	1701:1709	arg1	resistance					1756:1765	β-lactam resistance	1747:1765	β-lactam resistance responsible for the majority of treatment failures	1747:1816	IMPORTANCE Acinetobacter baumannii has become resistant to all classes of antibiotics, with β-lactam resistance responsible for the majority of treatment failures.
37074187	1	93	theme	cellular	250:257	arg1	function					259:266	a yet-to-be-identified cellular function	227:266	a yet-to-be-identified cellular function	227:266	The ADC (AmpC) β-lactamase is universally present in the Acinetobacter baumannii chromosome, suggesting it may have a yet-to-be-identified cellular function.
37074187	13	94	theme	A.	2124:2125	arg1	infections					2137:2146	A. baumannii infections	2124:2146	A. baumannii infections	2124:2146	Inhibitors of carbonic anhydrase could represent a new method for treating A. baumannii infections.
37074187	9	95	theme	cell	1445:1448	arg1	physiology					1450:1459	cell physiology	1445:1459	cell physiology	1445:1459	Our results highlight the impact of ADC-7 overexpression on cell physiology and reveal that the essential carbonic anhydrase CanB may represent a novel target for antimicrobial agents that would exhibit increased potency against β-lactamase-overexpressing A. baumannii.
35679150	12	0	theme	gene	1158:1161	arg1	sequence					1163:1170	16S rRNA gene sequence	1149:1170	16S rRNA gene sequence	1149:1170	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	17	1	theme	Catellatospora	2246:2259	arg1	sp					2269:2270	the name Catellatospora tritici sp	2237:2270	the name Catellatospora tritici sp	2237:2270	Therefore, strain NEAU-YM18T represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp.
35679150	7	2	theme	whole-cell	765:774	arg1	xylose					794:799	xylose	794:799	xylose	794:799	The whole-cell hydrolysates were xylose, glucose and ribose.
35679150	7	2	theme	whole-cell	765:774	arg1	hydrolysates					776:787	The whole-cell hydrolysates	761:787	The whole-cell hydrolysates	761:787	The whole-cell hydrolysates were xylose, glucose and ribose.
35679150	12	3	theme	sequence	1313:1320	arg1	T					1289:1289	T	1289:1289	T (98.4 % 16S rRNA gene sequence similarity)	1289:1332	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	12	3	theme	sequence	1313:1320	arg1	similarity					1322:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	9	4	theme	 1	986:987	arg1	ω9c					989:991	 1 ω9c	986:991	 1 ω9c	986:991	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	13	5	theme	genome	1499:1504	arg1	phylogeny					1506:1514	the whole genome phylogeny	1489:1514	the whole genome phylogeny of strain NEAU-YM18T	1489:1535	Furthermore, the whole genome phylogeny of strain NEAU-YM18T showed that the strain formed an independent clade.
35679150	14	6	theme	whole-genome	1780:1791	arg1	values					1821:1826	the whole-genome average nucleotide identity values	1776:1826	the whole-genome average nucleotide identity values between them	1776:1839	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	14	6	theme	whole-genome	1780:1791	arg1	%					1866:1866	81.5, 81.4 and 81.4 %	1846:1866	%	1866:1866	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	12	7	theme	%	1297:1297	arg1	T					1289:1289	T	1289:1289	T (98.4 % 16S rRNA gene sequence similarity)	1289:1332	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	12	7	theme	%	1297:1297	arg1	similarity					1322:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	5	8	theme	cell-wall	680:688	arg1	acids					696:700	diagnostic cell-wall amino acids	669:700	diagnostic cell-wall amino acids	669:700	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	8	theme	cell-wall	680:688	arg1	acids					660:664	3-hydroxy-diaminopimelic acids	635:664	3-hydroxy-diaminopimelic acids	635:664	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	8	theme	cell-wall	680:688	arg1	meso-					625:629	meso-	625:629	meso-	625:629	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	1	9	theme	Catellatospora	177:190	arg1	description					152:162	emended description	144:162	emended description of the genus Catellatospora	144:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	1	9	theme	Catellatospora	177:190	arg1	Triticum					118:125	Triticum	118:125	Triticum	118:125	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	1	9	theme	Catellatospora	177:190	arg1	wheat					111:115	wheat	111:115	wheat (Triticum aestivum L.)	111:138	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	12	10	theme	rRNA	1303:1306	arg1	T					1289:1289	T	1289:1289	T (98.4 % 16S rRNA gene sequence similarity)	1289:1332	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	12	10	theme	rRNA	1303:1306	arg1	similarity					1322:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	16	11	theme	genomic	2018:2024	arg1	analysis					2026:2033	genomic analysis	2018:2033	genomic analysis	2018:2033	In addition, genomic analysis confirmed that strain NEAU-YM18T had the potential to decompose cellulose and produce bioactive compounds.
35679150	17	12	theme	novel	2184:2188	arg1	species					2190:2196	a novel species	2182:2196	a novel species	2182:2196	Therefore, strain NEAU-YM18T represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp.
35679150	14	13	theme	DNA-DNA	1601:1607	arg1	hybridization					1609:1621	The digital DNA-DNA hybridization	1589:1621	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T	1589:1728	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	19	14	theme	2020040T=JCM	2332:2343	arg1	NEAU-YM18T					2310:2319	NEAU-YM18T	2310:2319	NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T)	2310:2351	The type strain is NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T).
35679150	19	14	theme	2020040T=JCM	2332:2343	arg1	33977T					2345:2350	=CCTCC AA 2020040T=JCM 33977T	2322:2350	=CCTCC AA 2020040T=JCM 33977T	2322:2350	The type strain is NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T).
35679150	16	15	theme	strain	2050:2055	arg1	NEAU-YM18T					2057:2066	strain NEAU-YM18T	2050:2066	strain NEAU-YM18T	2050:2066	In addition, genomic analysis confirmed that strain NEAU-YM18T had the potential to decompose cellulose and produce bioactive compounds.
35679150	2	16	theme	cellulose-degrading	210:228	arg1	Gram-positive					195:207	A Gram-positive	193:207	A Gram-positive	193:207	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	2	16	theme	cellulose-degrading	210:228	arg1	actinobacterium					230:244	cellulose-degrading actinobacterium	210:244	cellulose-degrading actinobacterium	210:244	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	17	17	theme	genus	2205:2209	arg1	Catellatospora					2211:2224	the genus Catellatospora	2201:2224	the genus Catellatospora	2201:2224	Therefore, strain NEAU-YM18T represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp.
35679150	2	18	dep	Triticum	320:327	arg1	L.					338:339	Triticum aestivum L.	320:339	Triticum aestivum L.	320:339	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	12	19	dep	related	1243:1249	arg1	%					1423:1423	98.3 %	1418:1423	98.3 %	1418:1423	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	14	20	dep	C.	1654:1655	arg1	T					1677:1677	2-25(1)T	1670:1677	C. chokoriensis 2-25(1)T	1654:1677	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	14	20	dep	C.	1654:1655	arg1	chokoriensis					1657:1668	C. chokoriensis 2-25(1)T	1654:1677	C. chokoriensis 2-25(1)T	1654:1677	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	2	21	theme	strain	256:261	arg1	NEAU-YM18T					263:272	designed strain NEAU-YM18T	247:272	designed strain NEAU-YM18T	247:272	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	2	21	theme	strain	256:261	arg1	Gram-positive					195:207	A Gram-positive	193:207	A Gram-positive	193:207	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	1	22	theme	description	152:162	arg1	soil					103:106	rhizosphere soil	91:106	rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora	91:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	2	23	theme	rhizosphere	293:303	arg1	soil					305:308	rhizosphere soil	293:308	rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China	293:386	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	17	24	theme	strain	2153:2158	arg1	NEAU-YM18T					2160:2169	strain NEAU-YM18T	2153:2169	strain NEAU-YM18T	2153:2169	Therefore, strain NEAU-YM18T represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp.
35679150	13	25	theme	independent	1570:1580	arg1	clade					1582:1586	an independent clade	1567:1586	an independent clade	1567:1586	Furthermore, the whole genome phylogeny of strain NEAU-YM18T showed that the strain formed an independent clade.
35679150	6	26	theme	acid	742:745	arg1	glycolyl					751:758	glycolyl	751:758	glycolyl	751:758	The acyl type of the cell-wall muramic acid was glycolyl.
35679150	6	26	theme	acid	742:745	arg1	type					712:715	The acyl type	703:715	The acyl type of the cell-wall muramic acid	703:745	The acyl type of the cell-wall muramic acid was glycolyl.
35679150	5	27	theme	NEAU-YM18T	589:598	arg1	Cells					573:577	Cells	573:577	Cells of strain NEAU-YM18T	573:598	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	6	28	theme	cell-wall	724:732	arg1	acid					742:745	the cell-wall muramic acid	720:745	the cell-wall muramic acid	720:745	The acyl type of the cell-wall muramic acid was glycolyl.
35679150	12	29	dep	chokoriensis	1269:1280	arg1	similarity					1322:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	12	29	dep	chokoriensis	1269:1280	arg1	T					1289:1289	T	1289:1289	T (98.4 % 16S rRNA gene sequence similarity)	1289:1332	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	0	30	theme	Catellatospora	0:13	arg1	sp					23:24	Catellatospora tritici sp	0:24	Catellatospora tritici sp.	0:25	Catellatospora tritici sp.
35679150	19	31	theme	=CCTCC AA	2322:2330	arg1	NEAU-YM18T					2310:2319	NEAU-YM18T	2310:2319	NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T)	2310:2351	The type strain is NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T).
35679150	19	31	theme	=CCTCC AA	2322:2330	arg1	33977T					2345:2350	=CCTCC AA 2020040T=JCM 33977T	2322:2350	=CCTCC AA 2020040T=JCM 33977T	2322:2350	The type strain is NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T).
35679150	14	32	theme	81.4 	1861:1865	arg1	values					1821:1826	the whole-genome average nucleotide identity values	1776:1826	the whole-genome average nucleotide identity values between them	1776:1839	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	14	32	theme	81.4 	1861:1865	arg1	%					1866:1866	81.5, 81.4 and 81.4 %	1846:1866	%	1866:1866	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	9	33	theme	fatty	938:942	arg1	 0					964:965	 0	964:965	 0	964:965	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	33	theme	fatty	938:942	arg1	iso-C15 					955:962	iso-C15 	955:962	iso-C15 	955:962	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	33	theme	fatty	938:942	arg1	acids					944:948	The major fatty acids	928:948	The major fatty acids	928:948	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	1	34	theme	wheat	111:115	arg1	soil					103:106	rhizosphere soil	91:106	rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora	91:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	3	35	theme	novel	393:397	arg1	strain					399:404	The novel strain	389:404	The novel strain	389:404	The novel strain was characterized using a polyphasic approach.
35679150	6	36	theme	acyl	707:710	arg1	glycolyl					751:758	glycolyl	751:758	glycolyl	751:758	The acyl type of the cell-wall muramic acid was glycolyl.
35679150	6	36	theme	acyl	707:710	arg1	type					712:715	The acyl type	703:715	The acyl type of the cell-wall muramic acid	703:745	The acyl type of the cell-wall muramic acid was glycolyl.
35679150	3	37	theme	polyphasic	432:441	arg1	approach					443:450	a polyphasic approach	430:450	a polyphasic approach	430:450	The novel strain was characterized using a polyphasic approach.
35679150	14	38	theme	81.5	1846:1849	arg1	values					1821:1826	the whole-genome average nucleotide identity values	1776:1826	the whole-genome average nucleotide identity values between them	1776:1839	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	14	38	theme	81.5	1846:1849	arg1	%					1866:1866	81.5, 81.4 and 81.4 %	1846:1866	%	1866:1866	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	12	39	theme	strain	1213:1218	arg1	NEAU-YM18T					1220:1229	strain NEAU-YM18T	1213:1229	strain NEAU-YM18T	1213:1229	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	13	40	theme	strain	1519:1524	arg1	NEAU-YM18T					1526:1535	strain NEAU-YM18T	1519:1535	strain NEAU-YM18T	1519:1535	Furthermore, the whole genome phylogeny of strain NEAU-YM18T showed that the strain formed an independent clade.
35679150	12	41	theme	sequence	1163:1170	arg1	results					1138:1144	The results	1134:1144	The results of 16S rRNA gene sequence and phylogenetic analyses	1134:1196	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	4	42	theme	genus	551:555	arg1	Catellatospora					557:570	the genus Catellatospora	547:570	the genus Catellatospora	547:570	Morphological and chemotaxonomic characteristics confirmed that strain NEAU-YM18T belonged to the genus Catellatospora.
35679150	11	43	theme	DNA	1106:1108	arg1	content					1114:1120	The DNA G+C content	1102:1120	The DNA G+C content	1102:1120	The DNA G+C content was 71.1 %.
35679150	11	43	theme	DNA	1106:1108	arg1	%					1131:1131	71.1 %	1126:1131	71.1 %	1126:1131	The DNA G+C content was 71.1 %.
35679150	12	44	theme	phylogenetic	1176:1187	arg1	analyses					1189:1196	phylogenetic analyses	1176:1196	phylogenetic analyses	1176:1196	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	9	45	dep	iso-C15 	955:962	arg1	 0					964:965	 0	964:965	 0	964:965	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	45	dep	iso-C15 	955:962	arg1	iso-C15 					955:962	iso-C15 	955:962	iso-C15 	955:962	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	45	dep	iso-C15 	955:962	arg1	acids					944:948	The major fatty acids	928:948	The major fatty acids	928:948	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	45	dep	iso-C15 	955:962	arg1	iso-C16 					968:975	iso-C16 	968:975	iso-C16 	968:975	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	14	46	theme	nucleotide	1801:1810	arg1	values					1821:1826	the whole-genome average nucleotide identity values	1776:1826	the whole-genome average nucleotide identity values between them	1776:1839	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	14	46	theme	nucleotide	1801:1810	arg1	%					1866:1866	81.5, 81.4 and 81.4 %	1846:1866	%	1866:1866	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	16	47	contain	had	2068:2070	arg2	potential					2076:2084	the potential to decompose cellulose and produce bioactive compounds	2072:2139	the potential to decompose cellulose and produce bioactive compounds	2072:2139	In addition, genomic analysis confirmed that strain NEAU-YM18T had the potential to decompose cellulose and produce bioactive compounds.
35679150	16	47	contain	had	2068:2070	arg1	NEAU-YM18T					2057:2066	strain NEAU-YM18T	2050:2066	strain NEAU-YM18T	2050:2066	In addition, genomic analysis confirmed that strain NEAU-YM18T had the potential to decompose cellulose and produce bioactive compounds.
35679150	17	48	theme	name	2241:2244	arg1	sp					2269:2270	the name Catellatospora tritici sp	2237:2270	the name Catellatospora tritici sp	2237:2270	Therefore, strain NEAU-YM18T represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp.
35679150	5	49	theme	amino	690:694	arg1	acids					696:700	diagnostic cell-wall amino acids	669:700	diagnostic cell-wall amino acids	669:700	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	49	theme	amino	690:694	arg1	acids					660:664	3-hydroxy-diaminopimelic acids	635:664	3-hydroxy-diaminopimelic acids	635:664	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	49	theme	amino	690:694	arg1	meso-					625:629	meso-	625:629	meso-	625:629	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	12	50	theme	rRNA	1153:1156	arg1	sequence					1163:1170	16S rRNA gene sequence	1149:1170	16S rRNA gene sequence	1149:1170	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	17	51	theme	tritici	2261:2267	arg1	sp					2269:2270	the name Catellatospora tritici sp	2237:2270	the name Catellatospora tritici sp	2237:2270	Therefore, strain NEAU-YM18T represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp.
35679150	13	52	theme	whole	1493:1497	arg1	phylogeny					1506:1514	the whole genome phylogeny	1489:1514	the whole genome phylogeny of strain NEAU-YM18T	1489:1535	Furthermore, the whole genome phylogeny of strain NEAU-YM18T showed that the strain formed an independent clade.
35679150	12	53	theme	gene	1308:1311	arg1	T					1289:1289	T	1289:1289	T (98.4 % 16S rRNA gene sequence similarity)	1289:1332	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	12	53	theme	gene	1308:1311	arg1	similarity					1322:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	14	54	theme	average	1793:1799	arg1	values					1821:1826	the whole-genome average nucleotide identity values	1776:1826	the whole-genome average nucleotide identity values between them	1776:1839	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	14	54	theme	average	1793:1799	arg1	%					1866:1866	81.5, 81.4 and 81.4 %	1846:1866	%	1866:1866	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	9	55	dep	 0	964:965	arg1	C18 					981:984	C18 	981:984	C18 	981:984	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	55	dep	 0	964:965	arg1	ω9c					989:991	 1 ω9c	986:991	 1 ω9c	986:991	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	55	dep	 0	964:965	arg1	feature					1004:1010	summed feature 5	997:1012	summed feature 5	997:1012	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	56	theme	summed	997:1002	arg1	feature					1004:1010	summed feature 5	997:1012	summed feature 5	997:1012	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	5	57	theme	diagnostic	669:678	arg1	acids					696:700	diagnostic cell-wall amino acids	669:700	diagnostic cell-wall amino acids	669:700	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	57	theme	diagnostic	669:678	arg1	acids					660:664	3-hydroxy-diaminopimelic acids	635:664	3-hydroxy-diaminopimelic acids	635:664	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	57	theme	diagnostic	669:678	arg1	meso-					625:629	meso-	625:629	meso-	625:629	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	1	58	theme	genus	171:175	arg1	Catellatospora					177:190	the genus Catellatospora	167:190	the genus Catellatospora	167:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	12	59	theme	98.4 	1292:1296	arg1	%					1297:1297	%	1297:1297	%	1297:1297	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	5	60	contain	contain	617:623	arg1	Cells					573:577	Cells	573:577	Cells of strain NEAU-YM18T	573:598	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	60	contain	contain	617:623	arg2	meso-					625:629	meso-	625:629	meso-	625:629	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	60	contain	contain	617:623	arg2	acids					696:700	diagnostic cell-wall amino acids	669:700	diagnostic cell-wall amino acids	669:700	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	60	contain	contain	617:623	arg2	acids					660:664	3-hydroxy-diaminopimelic acids	635:664	3-hydroxy-diaminopimelic acids	635:664	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	2	61	theme	wheat	313:317	arg1	soil					305:308	rhizosphere soil	293:308	rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China	293:386	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	12	62	theme	16S	1299:1301	arg1	T					1289:1289	T	1289:1289	T (98.4 % 16S rRNA gene sequence similarity)	1289:1332	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	12	62	theme	16S	1299:1301	arg1	similarity					1322:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	98.4 % 16S rRNA gene sequence similarity	1292:1331	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	1	63	attach	isolated	77:84	arg1	soil					103:106	rhizosphere soil	91:106	rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora	91:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	1	63	attach	isolated	77:84	arg2	nov.					27:30	nov.	27:30	nov.	27:30	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	1	63	attach	isolated	77:84	arg2	actinobacterium					61:75	a novel cellulase-producing actinobacterium	33:75	a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora	33:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	14	64	theme	hybridization	1609:1621	arg1	results					1623:1629	The digital DNA-DNA hybridization results	1589:1629	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T	1589:1728	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	2	65	theme	designed	247:254	arg1	NEAU-YM18T					263:272	designed strain NEAU-YM18T	247:272	designed strain NEAU-YM18T	247:272	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	2	65	theme	designed	247:254	arg1	Gram-positive					195:207	A Gram-positive	193:207	A Gram-positive	193:207	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	19	66	theme	type	2295:2298	arg1	NEAU-YM18T					2310:2319	NEAU-YM18T	2310:2319	NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T)	2310:2351	The type strain is NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T).
35679150	19	66	theme	type	2295:2298	arg1	strain					2300:2305	The type strain	2291:2305	The type strain	2291:2305	The type strain is NEAU-YM18T (=CCTCC AA 2020040T=JCM 33977T).
35679150	4	67	theme	Morphological	453:465	arg1	characteristics					486:500	Morphological and chemotaxonomic characteristics	453:500	Morphological and chemotaxonomic characteristics	453:500	Morphological and chemotaxonomic characteristics confirmed that strain NEAU-YM18T belonged to the genus Catellatospora.
35679150	14	68	theme	digital	1593:1599	arg1	hybridization					1609:1621	The digital DNA-DNA hybridization	1589:1621	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T	1589:1728	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	2	69	attach	isolated	279:286	arg1	soil					305:308	rhizosphere soil	293:308	rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China	293:386	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	2	69	attach	isolated	279:286	arg2	Gram-positive					195:207	A Gram-positive	193:207	A Gram-positive	193:207	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	2	69	attach	isolated	279:286	arg2	NEAU-YM18T					263:272	designed strain NEAU-YM18T	247:272	designed strain NEAU-YM18T	247:272	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	2	69	attach	isolated	279:286	arg2	actinobacterium					230:244	cellulose-degrading actinobacterium	210:244	cellulose-degrading actinobacterium	210:244	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	9	70	theme	 2	1036:1037	arg1	ω6,9c					1039:1043	 2 ω6,9c	1036:1043	anteiso-C18 : 0/C18 : 2 ω6,9c	1015:1043	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	5	71	theme	strain	582:587	arg1	NEAU-YM18T					589:598	strain NEAU-YM18T	582:598	strain NEAU-YM18T	582:598	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	17	72	theme	Catellatospora	2211:2224	arg1	species					2190:2196	a novel species	2182:2196	a novel species	2182:2196	Therefore, strain NEAU-YM18T represents a novel species of the genus Catellatospora, for which the name Catellatospora tritici sp.
35679150	4	73	theme	strain	517:522	arg1	NEAU-YM18T					524:533	strain NEAU-YM18T	517:533	strain NEAU-YM18T	517:533	Morphological and chemotaxonomic characteristics confirmed that strain NEAU-YM18T belonged to the genus Catellatospora.
35679150	1	74	theme	emended	144:150	arg1	description					152:162	emended description	144:162	emended description of the genus Catellatospora	144:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	1	75	theme	cellulase-producing	41:59	arg1	actinobacterium					61:75	a novel cellulase-producing actinobacterium	33:75	a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora	33:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	1	75	theme	cellulase-producing	41:59	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	4	76	theme	chemotaxonomic	471:484	arg1	characteristics					486:500	Morphological and chemotaxonomic characteristics	453:500	Morphological and chemotaxonomic characteristics	453:500	Morphological and chemotaxonomic characteristics confirmed that strain NEAU-YM18T belonged to the genus Catellatospora.
35679150	5	77	theme	3-hydroxy-diaminopimelic	635:658	arg1	acids					696:700	diagnostic cell-wall amino acids	669:700	diagnostic cell-wall amino acids	669:700	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	77	theme	3-hydroxy-diaminopimelic	635:658	arg1	acids					660:664	3-hydroxy-diaminopimelic acids	635:664	3-hydroxy-diaminopimelic acids	635:664	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	5	77	theme	3-hydroxy-diaminopimelic	635:658	arg1	meso-					625:629	meso-	625:629	meso-	625:629	Cells of strain NEAU-YM18T were observed to contain meso- and 3-hydroxy-diaminopimelic acids as diagnostic cell-wall amino acids.
35679150	11	78	theme	G+C	1110:1112	arg1	content					1114:1120	The DNA G+C content	1102:1120	The DNA G+C content	1102:1120	The DNA G+C content was 71.1 %.
35679150	11	78	theme	G+C	1110:1112	arg1	%					1131:1131	71.1 %	1126:1131	71.1 %	1126:1131	The DNA G+C content was 71.1 %.
35679150	1	79	dep	Triticum	118:125	arg1	L.					136:137	Triticum aestivum L.	118:137	Triticum aestivum L.	118:137	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	6	80	theme	muramic	734:740	arg1	acid					742:745	the cell-wall muramic acid	720:745	the cell-wall muramic acid	720:745	The acyl type of the cell-wall muramic acid was glycolyl.
35679150	0	81	theme	tritici	15:21	arg1	sp					23:24	Catellatospora tritici sp	0:24	Catellatospora tritici sp.	0:25	Catellatospora tritici sp.
35679150	9	82	dep	anteiso-C18 	1015:1026	arg1	 0/C18 					1028:1034	 0/C18 	1028:1034	anteiso-C18 : 0/C18 : 2 ω6,9c	1015:1043	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	82	dep	anteiso-C18 	1015:1026	arg1	ω6,9c					1039:1043	 2 ω6,9c	1036:1043	anteiso-C18 : 0/C18 : 2 ω6,9c	1015:1043	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	1	83	theme	rhizosphere	91:101	arg1	soil					103:106	rhizosphere soil	91:106	rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora	91:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	9	84	theme	major	932:936	arg1	 0					964:965	 0	964:965	 0	964:965	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	84	theme	major	932:936	arg1	iso-C15 					955:962	iso-C15 	955:962	iso-C15 	955:962	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	9	84	theme	major	932:936	arg1	acids					944:948	The major fatty acids	928:948	The major fatty acids	928:948	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	14	85	theme	identity	1812:1819	arg1	values					1821:1826	the whole-genome average nucleotide identity values	1776:1826	the whole-genome average nucleotide identity values between them	1776:1839	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	14	85	theme	identity	1812:1819	arg1	%					1866:1866	81.5, 81.4 and 81.4 %	1846:1866	%	1866:1866	The digital DNA-DNA hybridization results between NEAU-YM18T and C. chokoriensis 2-25(1)T, C. vulcania NEAU-JM1T and C. sichuanensis H14505T were 25.0, 24.7 and 24.7 %, respectively, and the whole-genome average nucleotide identity values between them were 81.5, 81.4 and 81.4 %, respectively.
35679150	15	86	theme	genetic	1889:1895	arg1	results					1897:1903	These genetic results	1883:1903	These genetic results	1883:1903	These genetic results and some phenotypic characteristics could distinguish strain NEAU-YM18T from its reference strains.
35679150	15	87	theme	phenotypic	1914:1923	arg1	characteristics					1925:1939	some phenotypic characteristics	1909:1939	some phenotypic characteristics	1909:1939	These genetic results and some phenotypic characteristics could distinguish strain NEAU-YM18T from its reference strains.
35679150	16	88	theme	bioactive	2121:2129	arg1	compounds					2131:2139	bioactive compounds	2121:2139	bioactive compounds	2121:2139	In addition, genomic analysis confirmed that strain NEAU-YM18T had the potential to decompose cellulose and produce bioactive compounds.
35679150	9	89	dep	ω9c	989:991	arg1	anteiso-C18 					1015:1026	anteiso-C18 	1015:1026	anteiso-C18 : 0/C18 : 2 ω6,9c	1015:1043	The major fatty acids were iso-C15 : 0, iso-C16 : 0, C18 : 1 ω9c and summed feature 5 (anteiso-C18 : 0/C18 : 2 ω6,9c).
35679150	15	90	theme	reference	1986:1994	arg1	strains					1996:2002	its reference strains	1982:2002	its reference strains	1982:2002	These genetic results and some phenotypic characteristics could distinguish strain NEAU-YM18T from its reference strains.
35679150	13	91	theme	NEAU-YM18T	1526:1535	arg1	phylogeny					1506:1514	the whole genome phylogeny	1489:1514	the whole genome phylogeny of strain NEAU-YM18T	1489:1535	Furthermore, the whole genome phylogeny of strain NEAU-YM18T showed that the strain formed an independent clade.
35679150	1	92	theme	novel	35:39	arg1	actinobacterium					61:75	a novel cellulase-producing actinobacterium	33:75	a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora	33:190	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	1	92	theme	novel	35:39	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a novel cellulase-producing actinobacterium isolated from rhizosphere soil of wheat (Triticum aestivum L.) and emended description of the genus Catellatospora.
35679150	12	93	theme	analyses	1189:1196	arg1	results					1138:1144	The results	1134:1144	The results of 16S rRNA gene sequence and phylogenetic analyses	1134:1196	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35679150	2	94	dep	Province	369:376	arg1	PR					379:380	PR	379:380	PR	379:380	A Gram-positive, cellulose-degrading actinobacterium, designed strain NEAU-YM18T, was isolated from rhizosphere soil of wheat (Triticum aestivum L.) sampled in Langfang, Hebei Province, PR China.
35679150	15	95	theme	strain	1959:1964	arg1	NEAU-YM18T					1966:1975	strain NEAU-YM18T	1959:1975	strain NEAU-YM18T	1959:1975	These genetic results and some phenotypic characteristics could distinguish strain NEAU-YM18T from its reference strains.
35679150	12	96	theme	16S	1149:1151	arg1	rRNA					1153:1156	16S rRNA	1149:1156	16S rRNA gene sequence	1149:1170	The results of 16S rRNA gene sequence and phylogenetic analyses indicated that strain NEAU-YM18T was closely related to Catellatospora chokoriensis 2-25(1)T (98.4 % 16S rRNA gene sequence similarity), Catellatospora vulcania NEAU-JM1T (98.3%) and Catellatospora sichuanensis H14505T (98.3 %) and formed a branch with C. sichuanensis H14505T.
35986008	7	0	theme	cell	1125:1128	arg1	line					1130:1133	a porcine cell line	1115:1133	a porcine cell line	1115:1133	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	6	1	theme	protein	939:945	arg1	motions					950:956	the asynchronous S protein D0 motions	920:956	the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain	920:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	6	2	theme	viral	968:972	arg1	particles					974:982	intact viral particles	961:982	intact viral particles of a highly virulent PEDV Pintung 52 strain	961:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	6	3	theme	Pintung	1010:1016	arg1	strain					1021:1026	a highly virulent PEDV Pintung 52 strain	987:1026	a highly virulent PEDV Pintung 52 strain	987:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	4	4	theme	atomic	527:532	arg1	structures					534:543	The atomic structures	523:543	The atomic structures of the recombinant PEDV S proteins of two different strains	523:603	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	8	5	theme	mass	1233:1236	arg1	spectrometry					1238:1249	mass spectrometry	1233:1249	mass spectrometry	1233:1249	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	7	6	theme	protein	1094:1100	arg1	structure					1063:1071	the cryo-EM structure	1051:1071	the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding	1051:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	8	7	theme	key	1403:1405	arg1	N-glycan					1407:1414	a key N-glycan	1401:1414	a key N-glycan	1401:1414	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	6	8	theme	PEDV	1005:1008	arg1	strain					1021:1026	a highly virulent PEDV Pintung 52 strain	987:1026	a highly virulent PEDV Pintung 52 strain	987:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	8	9	theme	S	1340:1340	arg1	N-glycans					1350:1358	the PEDV S protein N-glycans	1331:1358	the PEDV S protein N-glycans	1331:1358	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	6	10	from	motions	950:956	arg1	particles					974:982	intact viral particles	961:982	intact viral particles of a highly virulent PEDV Pintung 52 strain	961:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	8	11	theme	protein	1342:1348	arg1	N-glycans					1350:1358	the PEDV S protein N-glycans	1331:1358	the PEDV S protein N-glycans	1331:1358	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	3	12	theme	vaccine	479:485	arg1	development					487:497	vaccine development	479:497	vaccine development	479:497	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	2	13	theme	high	262:265	arg1	fatality					267:274	an exceptionally high fatality	245:274	an exceptionally high fatality in neonates	245:286	PED causes enteric disorders with an exceptionally high fatality in neonates, bringing substantial economic losses in the pork industry.
35986008	3	14	theme	spike	361:365	arg1	glycoprotein					371:382	The trimeric spike (S) glycoprotein	348:382	The trimeric spike (S) glycoprotein of PEDV	348:390	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	14	theme	spike	361:365	arg1	responsible					395:405	responsible	395:405	responsible	395:405	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	7	15	theme	porcine	1117:1123	arg1	line					1130:1133	a porcine cell line	1115:1133	a porcine cell line	1115:1133	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	6	16	theme	cryo-electron	823:835	arg1	cryo-ET					849:855	cryo-ET	849:855	cryo-ET	849:855	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	6	16	theme	cryo-electron	823:835	arg1	tomography					837:846	cryo-electron tomography	823:846	cryo-electron tomography (cryo-ET)	823:856	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	7	17	theme	additional	1151:1160	arg1	motions					1169:1175	additional domain motions	1151:1175	additional domain motions likely associated with receptor binding	1151:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	4	18	theme	domain	661:666	arg1	architectures					675:687	distinct N-terminal domain 0 (D0) architectures	641:687	distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states	641:738	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	2	19	theme	economic	310:317	arg1	losses					319:324	substantial economic losses	298:324	substantial economic losses in the pork industry	298:345	PED causes enteric disorders with an exceptionally high fatality in neonates, bringing substantial economic losses in the pork industry.
35986008	4	20	theme	proteins	571:578	arg1	structures					534:543	The atomic structures	523:543	The atomic structures of the recombinant PEDV S proteins of two different strains	523:603	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	1	21	theme	contagious	136:145	arg1	disease					153:159	a highly contagious swine disease	127:159	a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV)	127:208	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	21	theme	contagious	136:145	arg1	diarrhea					109:116	Porcine epidemic diarrhea	92:116	Porcine epidemic diarrhea (PED)	92:122	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	6	22	theme	asynchronous	924:935	arg1	motions					950:956	the asynchronous S protein D0 motions	920:956	the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain	920:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	3	23	theme	trimeric	352:359	arg1	glycoprotein					371:382	The trimeric spike (S) glycoprotein	348:382	The trimeric spike (S) glycoprotein of PEDV	348:390	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	23	theme	trimeric	352:359	arg1	responsible					395:405	responsible	395:405	responsible	395:405	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	1	24	theme	swine	147:151	arg1	disease					153:159	a highly contagious swine disease	127:159	a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV)	127:208	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	24	theme	swine	147:151	arg1	diarrhea					109:116	Porcine epidemic diarrhea	92:116	Porcine epidemic diarrhea (PED)	92:122	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	7	25	theme	S	1092:1092	arg1	protein					1094:1100	the recombinant S protein	1076:1100	the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding	1076:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	6	26	theme	virulent	996:1003	arg1	strain					1021:1026	a highly virulent PEDV Pintung 52 strain	987:1026	a highly virulent PEDV Pintung 52 strain	987:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	2	27	from	losses	319:324	arg1	industry					338:345	the pork industry	329:345	the pork industry	329:345	PED causes enteric disorders with an exceptionally high fatality in neonates, bringing substantial economic losses in the pork industry.
35986008	8	28	theme	D0	1434:1435	arg1	conformation					1437:1448	the D0 conformation	1430:1448	the D0 conformation	1430:1448	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	5	29	theme	alphacoronavirus	788:803	arg1	existence					745:753	The existence	741:753	The existence of the D0	741:763	The existence of the D0 is a unique feature of alphacoronavirus.
35986008	5	29	theme	alphacoronavirus	788:803	arg1	feature					777:783	a unique feature	768:783	a unique feature of alphacoronavirus	768:803	The existence of the D0 is a unique feature of alphacoronavirus.
35986008	0	30	theme	In	0:1	arg1	structure					8:16	In situ structure	0:16	In situ structure	0:16	In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM.
35986008	3	31	theme	antigenic	503:511	arg1	analysis					513:520	antigenic analysis	503:520	antigenic analysis	503:520	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	8	32	theme	complex	1282:1288	arg1	compositions					1290:1301	the complex compositions	1278:1301	the complex compositions	1278:1301	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	7	33	theme	domain	1162:1167	arg1	motions					1169:1175	additional domain motions	1151:1175	additional domain motions likely associated with receptor binding	1151:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	6	34	theme	intact	961:966	arg1	particles					974:982	intact viral particles	961:982	intact viral particles of a highly virulent PEDV Pintung 52 strain	961:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	3	35	theme	virus-host	411:420	arg1	fusion					444:449	membrane fusion	435:449	membrane fusion	435:449	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	35	theme	virus-host	411:420	arg1	recognition					422:432	virus-host recognition	411:432	virus-host recognition	411:432	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	7	36	theme	recombinant	1080:1090	arg1	protein					1094:1100	the recombinant S protein	1076:1100	the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding	1076:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	1	37	theme	Porcine	92:98	arg1	disease					153:159	a highly contagious swine disease	127:159	a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV)	127:208	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	37	theme	Porcine	92:98	arg1	PED					119:121	PED	119:121	PED	119:121	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	37	theme	Porcine	92:98	arg1	diarrhea					109:116	Porcine epidemic diarrhea	92:116	Porcine epidemic diarrhea (PED)	92:122	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	38	theme	porcine	171:177	arg1	PEDV					204:207	PEDV	204:207	PEDV	204:207	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	38	theme	porcine	171:177	arg1	virus					197:201	porcine epidemic diarrhea virus	171:201	porcine epidemic diarrhea virus (PEDV)	171:208	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	3	39	theme	S	368:368	arg1	glycoprotein					371:382	The trimeric spike (S) glycoprotein	348:382	The trimeric spike (S) glycoprotein of PEDV	348:390	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	39	theme	S	368:368	arg1	responsible					395:405	responsible	395:405	responsible	395:405	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	4	40	theme	distinct	641:648	arg1	architectures					675:687	distinct N-terminal domain 0 (D0) architectures	641:687	distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states	641:738	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	4	41	theme	different	587:595	arg1	strains					597:603	two different strains	583:603	two different strains	583:603	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	1	42	theme	epidemic	179:186	arg1	PEDV					204:207	PEDV	204:207	PEDV	204:207	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	42	theme	epidemic	179:186	arg1	virus					197:201	porcine epidemic diarrhea virus	171:201	porcine epidemic diarrhea virus (PEDV)	171:208	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	3	43	theme	PEDV	387:390	arg1	glycoprotein					371:382	The trimeric spike (S) glycoprotein	348:382	The trimeric spike (S) glycoprotein of PEDV	348:390	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	43	theme	PEDV	387:390	arg1	responsible					395:405	responsible	395:405	responsible	395:405	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	8	44	theme	functional	1382:1391	arg1	role					1393:1396	the functional role	1378:1396	the functional role of a key N-glycan in modulating the D0 conformation	1378:1448	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	6	45	theme	S	937:937	arg1	motions					950:956	the asynchronous S protein D0 motions	920:956	the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain	920:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	1	46	theme	diarrhea	188:195	arg1	PEDV					204:207	PEDV	204:207	PEDV	204:207	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	46	theme	diarrhea	188:195	arg1	virus					197:201	porcine epidemic diarrhea virus	171:201	porcine epidemic diarrhea virus (PEDV)	171:208	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	0	47	theme	alphacoronavirus	37:52	arg1	protein					60:66	an alphacoronavirus spike protein	34:66	an alphacoronavirus spike protein	34:66	In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM.
35986008	8	48	theme	PEDV	1335:1338	arg1	N-glycans					1350:1358	the PEDV S protein N-glycans	1331:1358	the PEDV S protein N-glycans	1331:1358	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	6	49	theme	strain	1021:1026	arg1	particles					974:982	intact viral particles	961:982	intact viral particles of a highly virulent PEDV Pintung 52 strain	961:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	1	50	theme	epidemic	100:107	arg1	disease					153:159	a highly contagious swine disease	127:159	a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV)	127:208	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	50	theme	epidemic	100:107	arg1	PED					119:121	PED	119:121	PED	119:121	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	1	50	theme	epidemic	100:107	arg1	diarrhea					109:116	Porcine epidemic diarrhea	92:116	Porcine epidemic diarrhea (PED)	92:122	Porcine epidemic diarrhea (PED) is a highly contagious swine disease caused by porcine epidemic diarrhea virus (PEDV).
35986008	7	51	theme	cryo-EM	1055:1061	arg1	structure					1063:1071	the cryo-EM structure	1051:1071	the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding	1051:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	6	52	theme	cryo-electron	862:874	arg1	microscopy					876:885	cryo-electron microscopy	862:885	cryo-electron microscopy (cryo-EM)	862:895	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	6	52	theme	cryo-electron	862:874	arg1	cryo-EM					888:894	cryo-EM	888:894	cryo-EM	888:894	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	8	53	theme	N-glycans	1350:1358	arg1	compositions					1290:1301	the complex compositions	1278:1301	the complex compositions	1278:1301	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	8	53	theme	N-glycans	1350:1358	arg1	distribution					1315:1326	spatial distribution	1307:1326	spatial distribution	1307:1326	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	2	54	theme	substantial	298:308	arg1	losses					319:324	substantial economic losses	298:324	substantial economic losses in the pork industry	298:345	PED causes enteric disorders with an exceptionally high fatality in neonates, bringing substantial economic losses in the pork industry.
35986008	0	55	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM.
35986008	4	56	theme	N-terminal	650:659	arg1	domain					661:666	N-terminal domain 0	650:668	distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states	641:738	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	4	56	theme	N-terminal	650:659	arg1	D0					671:672	D0	671:672	D0	671:672	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	4	57	theme	functional	722:731	arg1	states					733:738	different functional states	712:738	different functional states	712:738	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	2	58	theme	enteric	222:228	arg1	disorders					230:238	enteric disorders	222:238	enteric disorders	222:238	PED causes enteric disorders with an exceptionally high fatality in neonates, bringing substantial economic losses in the pork industry.
35986008	7	59	attach	derived	1102:1108	arg2	protein					1094:1100	the recombinant S protein	1076:1100	the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding	1076:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	7	59	attach	derived	1102:1108	arg1	line					1130:1133	a porcine cell line	1115:1133	a porcine cell line	1115:1133	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	8	60	theme	spatial	1307:1313	arg1	distribution					1315:1326	spatial distribution	1307:1326	spatial distribution	1307:1326	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	4	61	theme	recombinant	552:562	arg1	proteins					571:578	the recombinant PEDV S proteins	548:578	the recombinant PEDV S proteins of two different strains	548:603	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	7	62	theme	receptor	1200:1207	arg1	binding					1209:1215	receptor binding	1200:1215	receptor binding	1200:1215	We further determined the cryo-EM structure of the recombinant S protein derived from a porcine cell line, which revealed additional domain motions likely associated with receptor binding.
35986008	4	63	theme	different	712:720	arg1	states					733:738	different functional states	712:738	different functional states	712:738	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	8	64	theme	N-glycan	1407:1414	arg1	role					1393:1396	the functional role	1378:1396	the functional role of a key N-glycan in modulating the D0 conformation	1378:1448	By integrating mass spectrometry and cryo-EM, we delineated the complex compositions and spatial distribution of the PEDV S protein N-glycans, and demonstrated the functional role of a key N-glycan in modulating the D0 conformation.
35986008	3	65	theme	membrane	435:442	arg1	fusion					444:449	membrane fusion	435:449	membrane fusion	435:449	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	65	theme	membrane	435:442	arg1	recognition					422:432	virus-host recognition	411:432	virus-host recognition	411:432	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	6	66	theme	D0	947:948	arg1	motions					950:956	the asynchronous S protein D0 motions	920:956	the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain	920:1026	Here we combined cryo-electron tomography (cryo-ET) and cryo-electron microscopy (cryo-EM) to demonstrate in situ the asynchronous S protein D0 motions on intact viral particles of a highly virulent PEDV Pintung 52 strain.
35986008	0	67	theme	protein	60:66	arg1	structure					8:16	In situ structure	0:16	In situ structure	0:16	In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM.
35986008	0	67	theme	protein	60:66	arg1	dynamics					22:29	dynamics	22:29	dynamics	22:29	In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM.
35986008	5	68	theme	unique	770:775	arg1	existence					745:753	The existence	741:753	The existence of the D0	741:763	The existence of the D0 is a unique feature of alphacoronavirus.
35986008	5	68	theme	unique	770:775	arg1	feature					777:783	a unique feature	768:783	a unique feature of alphacoronavirus	768:803	The existence of the D0 is a unique feature of alphacoronavirus.
35986008	5	69	theme	D0	762:763	arg1	existence					745:753	The existence	741:753	The existence of the D0	741:763	The existence of the D0 is a unique feature of alphacoronavirus.
35986008	5	69	theme	D0	762:763	arg1	feature					777:783	a unique feature	768:783	a unique feature of alphacoronavirus	768:803	The existence of the D0 is a unique feature of alphacoronavirus.
35986008	2	70	theme	pork	333:336	arg1	industry					338:345	the pork industry	329:345	the pork industry	329:345	PED causes enteric disorders with an exceptionally high fatality in neonates, bringing substantial economic losses in the pork industry.
35986008	4	71	theme	strains	597:603	arg1	proteins					571:578	the recombinant PEDV S proteins	548:578	the recombinant PEDV S proteins of two different strains	548:603	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	4	72	theme	S	569:569	arg1	proteins					571:578	the recombinant PEDV S proteins	548:578	the recombinant PEDV S proteins of two different strains	548:603	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
35986008	0	73	theme	spike	54:58	arg1	protein					60:66	an alphacoronavirus spike protein	34:66	an alphacoronavirus spike protein	34:66	In situ structure and dynamics of an alphacoronavirus spike protein by cryo-ET and cryo-EM.
35986008	3	74	gly	glycoprotein	371:382	arg1	glycoprotein					371:382	The trimeric spike (S) glycoprotein	348:382	The trimeric spike (S) glycoprotein of PEDV	348:390	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	74	gly	glycoprotein	371:382	arg1	responsible					395:405	responsible	395:405	responsible	395:405	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	3	75	theme	main	463:466	arg1	target					468:473	the main target	459:473	the main target for vaccine development and antigenic analysis	459:520	The trimeric spike (S) glycoprotein of PEDV is responsible for virus-host recognition, membrane fusion, and is the main target for vaccine development and antigenic analysis.
35986008	2	76	from	fatality	267:274	arg1	neonates					279:286	neonates	279:286	neonates	279:286	PED causes enteric disorders with an exceptionally high fatality in neonates, bringing substantial economic losses in the pork industry.
35986008	4	77	theme	PEDV	564:567	arg1	proteins					571:578	the recombinant PEDV S proteins	548:578	the recombinant PEDV S proteins of two different strains	548:603	The atomic structures of the recombinant PEDV S proteins of two different strains have been reported, but they reveal distinct N-terminal domain 0 (D0) architectures that may correspond to different functional states.
36771448	8	0	theme	LPS	1029:1031	arg1	levels					1033:1038	the liver LPS levels	1019:1038	the liver LPS levels	1019:1038	SFN decreased the liver LPS levels and inhibited the LPS/TLR4 pathway activations, thus inhibiting the pro-inflammatory cytokines.
36771448	10	1	theme	dietary	1356:1362	arg1	intervention					1364:1375	dietary intervention	1356:1375	dietary intervention with SFN	1356:1384	Above all, dietary intervention with SFN attenuates NAFLD through the gut-liver axis.
36771448	9	2	theme	intestinal	1219:1228	arg1	integrity					1238:1246	intestinal barrier integrity	1219:1246	intestinal barrier integrity	1219:1246	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	3	3	theme	high-fructose	447:459	arg1	diet					469:472	a high-fat and high-fructose (HFHFr) diet	432:472	a high-fat and high-fructose (HFHFr) diet	432:472	C57BL/6 mice were fed a high-fat and high-fructose (HFHFr) diet, with or without SFN gavage at doses of 15 and 30 mg·kg-1 body weight for 12 weeks.
36771448	9	4	theme	improved	1314:1321	arg1	dysbiosis					1334:1342	improved intestinal dysbiosis	1314:1342	improved intestinal dysbiosis	1314:1342	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	0	5	theme	High-Fructose	82:94	arg1	Diet					96:99	High-Fat and High-Fructose Diet	69:99	High-Fat and High-Fructose Diet	69:99	Sulforaphane Ameliorates Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis.
36771448	9	6	theme	protective	1191:1200	arg1	effect					1202:1207	the protective effect	1187:1207	the protective effect of SFN on intestinal barrier integrity	1187:1246	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	4	7	theme	weight	594:599	arg1	gain					601:604	weight gain	594:604	weight gain	594:604	The results showed that SFN reduced weight gain, hepatic inflammation, and steatosis in HFHFr mice.
36771448	9	8	theme	intestinal	1323:1332	arg1	dysbiosis					1334:1342	improved intestinal dysbiosis	1314:1342	improved intestinal dysbiosis	1314:1342	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	7	9	from	translocations	949:962	arg1	mice					999:1002	HFHFr diet-induced mice	980:1002	HFHFr diet-induced mice	980:1002	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	7	10	theme	diet-induced	986:997	arg1	mice					999:1002	HFHFr diet-induced mice	980:1002	HFHFr diet-induced mice	980:1002	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	6	11	dep	LPS/TLR4	803:810	arg1	pathways					820:827	pathways	820:827	pathways	820:827	Moreover, SFN enhanced the intestinal tight junction protein ZO-1, reduced serum LPS, and inhibited LPS/TLR4 and ERS pathways to reduce intestinal inflammation.
36771448	9	12	from	effect	1274:1279	arg1	liver					1288:1292	the liver	1284:1292	the liver	1284:1292	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	9	12	from	effect	1274:1279	arg1	integrity					1238:1246	intestinal barrier integrity	1219:1246	intestinal barrier integrity	1219:1246	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	4	13	theme	hepatic	607:613	arg1	inflammation					615:626	hepatic inflammation	607:626	hepatic inflammation	607:626	The results showed that SFN reduced weight gain, hepatic inflammation, and steatosis in HFHFr mice.
36771448	2	14	located	found	292:296	arg2	compound					283:290	a bioactive compound	271:290	a bioactive compound found in cruciferous vegetables	271:322	Sulforaphane (SFN) is a bioactive compound found in cruciferous vegetables; however, it has not been reported whether SFN improves NAFLD via the gut-liver axis.
36771448	2	14	located	found	292:296	arg2	Sulforaphane					249:260	Sulforaphane	249:260	Sulforaphane (SFN)	249:266	Sulforaphane (SFN) is a bioactive compound found in cruciferous vegetables; however, it has not been reported whether SFN improves NAFLD via the gut-liver axis.
36771448	2	14	located	found	292:296	arg1	vegetables					313:322	cruciferous vegetables	301:322	cruciferous vegetables	301:322	Sulforaphane (SFN) is a bioactive compound found in cruciferous vegetables; however, it has not been reported whether SFN improves NAFLD via the gut-liver axis.
36771448	7	15	theme	HFHFr	980:984	arg1	mice					999:1002	HFHFr diet-induced mice	980:1002	HFHFr diet-induced mice	980:1002	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	8	16	theme	LPS/TLR4	1058:1065	arg1	activations					1075:1085	the LPS/TLR4 pathway activations	1054:1085	the LPS/TLR4 pathway activations	1054:1085	SFN decreased the liver LPS levels and inhibited the LPS/TLR4 pathway activations, thus inhibiting the pro-inflammatory cytokines.
36771448	5	17	theme	microbes	693:700	arg1	composition					674:684	the composition	670:684	the composition of gut microbes	670:700	SFN altered the composition of gut microbes.
36771448	8	18	theme	liver	1023:1027	arg1	levels					1033:1038	the liver LPS levels	1019:1038	the liver LPS levels	1019:1038	SFN decreased the liver LPS levels and inhibited the LPS/TLR4 pathway activations, thus inhibiting the pro-inflammatory cytokines.
36771448	6	19	theme	serum	778:782	arg1	LPS					784:786	serum LPS	778:786	serum LPS	778:786	Moreover, SFN enhanced the intestinal tight junction protein ZO-1, reduced serum LPS, and inhibited LPS/TLR4 and ERS pathways to reduce intestinal inflammation.
36771448	1	20	theme	key	173:175	arg1	player					177:182	a key player	171:182	a key player in the progression of non-alcoholic fatty liver disease (NAFLD)	171:246	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	2	21	theme	gut-liver	394:402	arg1	axis					404:407	the gut-liver axis	390:407	the gut-liver axis	390:407	Sulforaphane (SFN) is a bioactive compound found in cruciferous vegetables; however, it has not been reported whether SFN improves NAFLD via the gut-liver axis.
36771448	9	22	theme	barrier	1230:1236	arg1	integrity					1238:1246	intestinal barrier integrity	1219:1246	intestinal barrier integrity	1219:1246	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	2	23	theme	bioactive	273:281	arg1	Sulforaphane					249:260	Sulforaphane	249:260	Sulforaphane (SFN)	249:266	Sulforaphane (SFN) is a bioactive compound found in cruciferous vegetables; however, it has not been reported whether SFN improves NAFLD via the gut-liver axis.
36771448	2	23	theme	bioactive	273:281	arg1	compound					283:290	a bioactive compound	271:290	a bioactive compound found in cruciferous vegetables	271:322	Sulforaphane (SFN) is a bioactive compound found in cruciferous vegetables; however, it has not been reported whether SFN improves NAFLD via the gut-liver axis.
36771448	0	24	theme	Fatty	38:42	arg1	Disease					50:56	Nonalcoholic Fatty Liver Disease	25:56	Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis	25:134	Sulforaphane Ameliorates Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis.
36771448	10	25	theme	gut-liver	1415:1423	arg1	axis					1425:1428	the gut-liver axis	1411:1428	the gut-liver axis	1411:1428	Above all, dietary intervention with SFN attenuates NAFLD through the gut-liver axis.
36771448	4	26	theme	HFHFr	646:650	arg1	mice					652:655	HFHFr mice	646:655	HFHFr mice	646:655	The results showed that SFN reduced weight gain, hepatic inflammation, and steatosis in HFHFr mice.
36771448	10	27	with	intervention	1364:1375	arg1	SFN					1382:1384	SFN	1382:1384	SFN	1382:1384	Above all, dietary intervention with SFN attenuates NAFLD through the gut-liver axis.
36771448	0	28	theme	Nonalcoholic	25:36	arg1	Disease					50:56	Nonalcoholic Fatty Liver Disease	25:56	Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis	25:134	Sulforaphane Ameliorates Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis.
36771448	6	29	theme	intestinal	839:848	arg1	inflammation					850:861	intestinal inflammation	839:861	intestinal inflammation	839:861	Moreover, SFN enhanced the intestinal tight junction protein ZO-1, reduced serum LPS, and inhibited LPS/TLR4 and ERS pathways to reduce intestinal inflammation.
36771448	0	30	theme	Gut-Liver	121:129	arg1	Axis					131:134	the Gut-Liver Axis	117:134	the Gut-Liver Axis	117:134	Sulforaphane Ameliorates Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis.
36771448	3	31	theme	SFN	491:493	arg1	gavage					495:500	SFN gavage	491:500	SFN gavage	491:500	C57BL/6 mice were fed a high-fat and high-fructose (HFHFr) diet, with or without SFN gavage at doses of 15 and 30 mg·kg-1 body weight for 12 weeks.
36771448	7	32	link	gut-derived	933:943	arg1	LPS					945:947	the gut-derived LPS	929:947	the gut-derived LPS	929:947	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	7	33	theme	gut-derived	933:943	arg1	LPS					945:947	the gut-derived LPS	929:947	the gut-derived LPS	929:947	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	6	34	theme	protein	756:762	arg1	ZO-1					764:767	the intestinal tight junction protein ZO-1	726:767	the intestinal tight junction protein ZO-1	726:767	Moreover, SFN enhanced the intestinal tight junction protein ZO-1, reduced serum LPS, and inhibited LPS/TLR4 and ERS pathways to reduce intestinal inflammation.
36771448	0	35	theme	Liver	44:48	arg1	Disease					50:56	Nonalcoholic Fatty Liver Disease	25:56	Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis	25:134	Sulforaphane Ameliorates Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis.
36771448	9	36	theme	anti-inflammatory	1256:1272	arg1	effect					1274:1279	its anti-inflammatory effect	1252:1279	its anti-inflammatory effect on the liver	1252:1292	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	9	37	from	effect	1202:1207	arg1	liver					1288:1292	the liver	1284:1292	the liver	1284:1292	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	9	37	from	effect	1202:1207	arg1	integrity					1238:1246	intestinal barrier integrity	1219:1246	intestinal barrier integrity	1219:1246	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	6	38	theme	junction	747:754	arg1	ZO-1					764:767	the intestinal tight junction protein ZO-1	726:767	the intestinal tight junction protein ZO-1	726:767	Moreover, SFN enhanced the intestinal tight junction protein ZO-1, reduced serum LPS, and inhibited LPS/TLR4 and ERS pathways to reduce intestinal inflammation.
36771448	3	39	theme	C57BL/6	410:416	arg1	mice					418:421	C57BL/6 mice	410:421	C57BL/6 mice	410:421	C57BL/6 mice were fed a high-fat and high-fructose (HFHFr) diet, with or without SFN gavage at doses of 15 and 30 mg·kg-1 body weight for 12 weeks.
36771448	8	40	theme	pro-inflammatory	1108:1123	arg1	cytokines					1125:1133	the pro-inflammatory cytokines	1104:1133	the pro-inflammatory cytokines	1104:1133	SFN decreased the liver LPS levels and inhibited the LPS/TLR4 pathway activations, thus inhibiting the pro-inflammatory cytokines.
36771448	6	41	theme	tight	741:745	arg1	ZO-1					764:767	the intestinal tight junction protein ZO-1	726:767	the intestinal tight junction protein ZO-1	726:767	Moreover, SFN enhanced the intestinal tight junction protein ZO-1, reduced serum LPS, and inhibited LPS/TLR4 and ERS pathways to reduce intestinal inflammation.
36771448	7	42	theme	intestinal	895:904	arg1	result					869:874	a result	867:874	a result	867:874	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	7	42	theme	intestinal	895:904	arg1	integrity					906:914	the intestinal integrity	891:914	the intestinal integrity	891:914	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	7	42	theme	intestinal	895:904	arg1	translocations					949:962	translocations	949:962	translocations to the liver in HFHFr diet-induced mice	949:1002	As a result, SFN protected the intestinal integrity and declined the gut-derived LPS translocations to the liver in HFHFr diet-induced mice.
36771448	3	43	theme	high-fat	434:441	arg1	diet					469:472	a high-fat and high-fructose (HFHFr) diet	432:472	a high-fat and high-fructose (HFHFr) diet	432:472	C57BL/6 mice were fed a high-fat and high-fructose (HFHFr) diet, with or without SFN gavage at doses of 15 and 30 mg·kg-1 body weight for 12 weeks.
36771448	5	44	theme	gut	689:691	arg1	microbes					693:700	gut microbes	689:700	gut microbes	689:700	SFN altered the composition of gut microbes.
36771448	9	45	theme	Spearman	1145:1152	arg1	analysis					1166:1173	Spearman correlation analysis	1145:1173	Spearman correlation analysis	1145:1173	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	6	46	theme	intestinal	730:739	arg1	ZO-1					764:767	the intestinal tight junction protein ZO-1	726:767	the intestinal tight junction protein ZO-1	726:767	Moreover, SFN enhanced the intestinal tight junction protein ZO-1, reduced serum LPS, and inhibited LPS/TLR4 and ERS pathways to reduce intestinal inflammation.
36771448	2	47	theme	cruciferous	301:311	arg1	vegetables					313:322	cruciferous vegetables	301:322	cruciferous vegetables	301:322	Sulforaphane (SFN) is a bioactive compound found in cruciferous vegetables; however, it has not been reported whether SFN improves NAFLD via the gut-liver axis.
36771448	9	48	theme	correlation	1154:1164	arg1	analysis					1166:1173	Spearman correlation analysis	1145:1173	Spearman correlation analysis	1145:1173	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	3	49	theme	body	532:535	arg1	weight					537:542	15 and 30 mg·kg-1 body weight	514:542	15 and 30 mg·kg-1 body weight	514:542	C57BL/6 mice were fed a high-fat and high-fructose (HFHFr) diet, with or without SFN gavage at doses of 15 and 30 mg·kg-1 body weight for 12 weeks.
36771448	9	50	theme	SFN	1212:1214	arg1	effect					1274:1279	its anti-inflammatory effect	1252:1279	its anti-inflammatory effect on the liver	1252:1292	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	9	50	theme	SFN	1212:1214	arg1	effect					1202:1207	the protective effect	1187:1207	the protective effect of SFN on intestinal barrier integrity	1187:1246	Notably, Spearman correlation analysis showed that the protective effect of SFN on intestinal barrier integrity and its anti-inflammatory effect on the liver was associated with improved intestinal dysbiosis.
36771448	1	51	theme	non-alcoholic	206:218	arg1	NAFLD					241:245	NAFLD	241:245	NAFLD	241:245	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	1	51	theme	non-alcoholic	206:218	arg1	disease					232:238	non-alcoholic fatty liver disease	206:238	non-alcoholic fatty liver disease (NAFLD)	206:246	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	3	52	theme	weight	537:542	arg1	doses					505:509	doses	505:509	doses of 15 and 30 mg·kg-1 body weight	505:542	C57BL/6 mice were fed a high-fat and high-fructose (HFHFr) diet, with or without SFN gavage at doses of 15 and 30 mg·kg-1 body weight for 12 weeks.
36771448	1	53	theme	fatty	220:224	arg1	NAFLD					241:245	NAFLD	241:245	NAFLD	241:245	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	1	53	theme	fatty	220:224	arg1	disease					232:238	non-alcoholic fatty liver disease	206:238	non-alcoholic fatty liver disease (NAFLD)	206:246	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	1	54	from	player	177:182	arg1	progression					191:201	the progression	187:201	the progression of non-alcoholic fatty liver disease (NAFLD)	187:246	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	1	55	theme	gut-liver	141:149	arg1	axis					151:154	The gut-liver axis	137:154	The gut-liver axis	137:154	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	1	56	theme	liver	226:230	arg1	NAFLD					241:245	NAFLD	241:245	NAFLD	241:245	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	1	56	theme	liver	226:230	arg1	disease					232:238	non-alcoholic fatty liver disease	206:238	non-alcoholic fatty liver disease (NAFLD)	206:246	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36771448	0	57	theme	High-Fat	69:76	arg1	Diet					96:99	High-Fat and High-Fructose Diet	69:99	High-Fat and High-Fructose Diet	69:99	Sulforaphane Ameliorates Nonalcoholic Fatty Liver Disease Induced by High-Fat and High-Fructose Diet via LPS/TLR4 in the Gut-Liver Axis.
36771448	3	58	theme	mg·kg-1	524:530	arg1	weight					537:542	15 and 30 mg·kg-1 body weight	514:542	15 and 30 mg·kg-1 body weight	514:542	C57BL/6 mice were fed a high-fat and high-fructose (HFHFr) diet, with or without SFN gavage at doses of 15 and 30 mg·kg-1 body weight for 12 weeks.
36771448	8	59	theme	pathway	1067:1073	arg1	activations					1075:1085	the LPS/TLR4 pathway activations	1054:1085	the LPS/TLR4 pathway activations	1054:1085	SFN decreased the liver LPS levels and inhibited the LPS/TLR4 pathway activations, thus inhibiting the pro-inflammatory cytokines.
36771448	1	60	theme	disease	232:238	arg1	progression					191:201	the progression	187:201	the progression of non-alcoholic fatty liver disease (NAFLD)	187:246	The gut-liver axis has emerged as a key player in the progression of non-alcoholic fatty liver disease (NAFLD).
36268862	6	0	theme	average	778:784	arg1	identity					797:804	average nucleotide identity	778:804	average nucleotide identity	778:804	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	12	1	theme	phenotypic	1943:1952	arg1	data					1954:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	4	2	theme	diffusible	541:550	arg1	pigments					552:559	no diffusible pigments	538:559	no diffusible pigments	538:559	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
36268862	7	3	theme	strain	1076:1081	arg1	CMS5P-6T					1083:1090	strain CMS5P-6T	1076:1090	strain CMS5P-6T	1076:1090	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	9	4	theme	DNA	1373:1375	arg1	%					1437:1437	65.6 mol%	1429:1437	65.6 mol%	1429:1437	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	9	4	theme	DNA	1373:1375	arg1	content					1381:1387	The DNA G+C content	1369:1387	The DNA G+C content of strain CMS5P-6T	1369:1406	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	6	5	theme	identity	860:867	arg1	%					949:949	78.0, 21.7 and 70.8 %	929:949	%	949:949	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	6	5	theme	identity	860:867	arg1	values					869:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	774:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T	774:922	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	10	6	theme	respiratory	1587:1597	arg1	lipoquinone					1599:1609	the respiratory lipoquinone	1583:1609	the respiratory lipoquinone	1583:1609	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	6	theme	respiratory	1587:1597	arg1	acid					1540:1543	the diagnostic diamino acid	1517:1543	the diagnostic diamino acid	1517:1543	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	6	theme	respiratory	1587:1597	arg1	Q-10					1560:1563	ubiquinone Q-10	1549:1563	ubiquinone Q-10	1549:1563	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	14	7	theme	type	2112:2115	arg1	CMS5P-6T					2127:2134	CMS5P-6T	2127:2134	CMS5P-6T (=JCM 33125T=CGMCC 1.13868T)	2127:2163	The type strain is CMS5P-6T (=JCM 33125T=CGMCC 1.13868T).
36268862	14	7	theme	type	2112:2115	arg1	strain					2117:2122	The type strain	2108:2122	The type strain	2108:2122	The type strain is CMS5P-6T (=JCM 33125T=CGMCC 1.13868T).
36268862	2	8	theme	Zhuang	273:278	arg1	Region					291:296	Guangxi Zhuang Autonomous Region	265:296	Guangxi Zhuang Autonomous Region	265:296	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	2	8	theme	Zhuang	273:278	arg1	China					302:306	PR China	299:306	PR China	299:306	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	12	9	theme	genomic	1915:1921	arg1	data					1954:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	6	10	theme	amino	849:853	arg1	identity					860:867	average amino acid identity	841:867	average amino acid identity	841:867	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	12	11	theme	Hephaestia	2066:2075	arg1	sp					2086:2087	the name Hephaestia mangrovi sp	2057:2087	the name Hephaestia mangrovi sp	2057:2087	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	7	12	theme	acid	1047:1050	arg1	identity					1052:1059	average amino acid identity	1033:1059	average amino acid identity	1033:1059	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	9	13	theme	strain	1392:1397	arg1	CMS5P-6T					1399:1406	strain CMS5P-6T	1392:1406	strain CMS5P-6T	1392:1406	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	12	14	theme	chemotaxonomic	1924:1937	arg1	data					1954:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	12	15	theme	Hephaestia	2035:2044	arg1	species					2014:2020	a novel species	2006:2020	a novel species	2006:2020	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	10	16	theme	diamino	1532:1538	arg1	lipoquinone					1599:1609	the respiratory lipoquinone	1583:1609	the respiratory lipoquinone	1583:1609	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	16	theme	diamino	1532:1538	arg1	acid					1540:1543	the diagnostic diamino acid	1517:1543	the diagnostic diamino acid	1517:1543	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	16	theme	diamino	1532:1538	arg1	Q-10					1560:1563	ubiquinone Q-10	1549:1563	ubiquinone Q-10	1549:1563	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	7	17	theme	average	1033:1039	arg1	identity					1052:1059	average amino acid identity	1033:1059	average amino acid identity	1033:1059	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	2	18	theme	PR	299:300	arg1	Region					291:296	Guangxi Zhuang Autonomous Region	265:296	Guangxi Zhuang Autonomous Region	265:296	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	2	18	theme	PR	299:300	arg1	China					302:306	PR China	299:306	PR China	299:306	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	7	19	theme	hybridization	1015:1027	arg1	%					1146:1146	74.0, 19.9 and 61.4 %	1126:1146	%	1146:1146	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	7	19	theme	hybridization	1015:1027	arg1	values					1061:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	966:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T	966:1119	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	12	20	theme	phylogenetic	1901:1912	arg1	data					1954:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	phylogenetic, genomic, chemotaxonomic and phenotypic data	1901:1957	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	6	21	theme	DSM	913:915	arg1	25527T					917:922	H. caeni DSM 25527T	904:922	H. caeni DSM 25527T	904:922	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	12	22	theme	strain	1960:1965	arg1	CMS5P-6T					1967:1974	strain CMS5P-6T	1960:1974	strain CMS5P-6T	1960:1974	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	11	23	theme	fatty	1811:1815	arg1	acids					1817:1821	the major fatty acids	1801:1821	the major fatty acids	1801:1821	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	11	23	theme	fatty	1811:1815	arg1	C16 					1876:1879	C16 	1876:1879	C16 	1876:1879	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	11	23	theme	fatty	1811:1815	arg1	C18 					1842:1845	C18 	1842:1845	C18 	1842:1845	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	7	24	theme	digital	999:1005	arg1	hybridization					1015:1027	digital DNA-DNA hybridization	999:1027	digital DNA-DNA hybridization	999:1027	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	2	25	theme	surface-sterilized	200:217	arg1	bark					219:222	a surface-sterilized bark	198:222	a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China,	198:307	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	9	26	theme	65.6 mol	1429:1436	arg1	%					1437:1437	65.6 mol%	1429:1437	65.6 mol%	1429:1437	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	9	26	theme	65.6 mol	1429:1436	arg1	content					1381:1387	The DNA G+C content	1369:1387	The DNA G+C content of strain CMS5P-6T	1369:1406	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	8	27	theme	DSM	1252:1254	arg1	25527T					1256:1261	DSM 25527T	1252:1261	DSM 25527T	1252:1261	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	7	28	theme	identity	989:996	arg1	%					1146:1146	74.0, 19.9 and 61.4 %	1126:1146	%	1146:1146	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	7	28	theme	identity	989:996	arg1	values					1061:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	966:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T	966:1119	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	5	29	theme	Hephaestia	705:714	arg1	DSM					722:724	Hephaestia caeni DSM 25527T	705:731	Hephaestia caeni DSM 25527T	705:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	2	30	theme	corniculatum	237:248	arg1	bark					219:222	a surface-sterilized bark	198:222	a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China,	198:307	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	5	31	theme	high	653:656	arg1	similarity					681:690	high 16S rRNA gene sequence similarity	653:690	high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T	653:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	8	32	theme	distinct	1272:1279	arg1	cluster					1281:1287	a distinct cluster	1270:1287	a distinct cluster	1270:1287	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	11	33	theme	unidentified	1771:1782	arg1	aminolipids					1784:1794	two unidentified aminolipids	1767:1794	two unidentified aminolipids	1767:1794	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	11	34	dep	C18 	1842:1845	arg1	C19 					1855:1858	C19 	1855:1858	C19 	1855:1858	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	11	34	dep	C18 	1842:1845	arg1	cycloω8c					1863:1870	 0 cycloω8c	1860:1870	C18 : 1 ω7c, C19 : 0 cycloω8c	1842:1870	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	7	35	dep	S.	1096:1097	arg1	colocasiea					1099:1108	colocasiea	1099:1108	colocasiea	1099:1108	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	8	36	theme	family	1300:1305	arg1	Sphingomonadaceae					1307:1323	the family Sphingomonadaceae	1296:1323	the family Sphingomonadaceae	1296:1323	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	5	37	theme	%	700:700	arg1	similarity					681:690	high 16S rRNA gene sequence similarity	653:690	high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T	653:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	5	37	theme	%	700:700	arg1	CC-MHH0539T					761:771	Sphingomonas colocasiea CC-MHH0539T	737:771	Sphingomonas colocasiea CC-MHH0539T	737:771	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	5	38	theme	gene	667:670	arg1	similarity					681:690	high 16S rRNA gene sequence similarity	653:690	high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T	653:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	0	39	theme	Hephaestia	0:9	arg1	sp					20:21	Hephaestia mangrovi sp	0:21	Hephaestia mangrovi sp.	0:22	Hephaestia mangrovi sp.
36268862	2	40	attach	isolated	184:191	arg2	bacterium					148:156	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium	97:156	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium	97:156	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	2	40	attach	isolated	184:191	arg1	bark					219:222	a surface-sterilized bark	198:222	a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China,	198:307	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	7	41	theme	74.0	1126:1129	arg1	%					1146:1146	74.0, 19.9 and 61.4 %	1126:1146	%	1146:1146	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	7	41	theme	74.0	1126:1129	arg1	values					1061:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	966:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T	966:1119	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	6	42	theme	average	841:847	arg1	identity					860:867	average amino acid identity	841:867	average amino acid identity	841:867	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	8	43	theme	S.	1343:1344	arg1	31229T					1361:1366	S. colocasiea JCM 31229T	1343:1366	S. colocasiea JCM 31229T	1343:1366	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	3	44	theme	%	441:441	arg1	NaCl					449:452	0-1 % (w/v) NaCl	437:452	0-1 % (w/v) NaCl	437:452	Strain CMS5P-6T was found to grow optimally with 0-1 % (w/v) NaCl, at 30 °C and pH 6.0-7.0.
36268862	6	45	theme	hybridization	823:835	arg1	%					949:949	78.0, 21.7 and 70.8 %	929:949	%	949:949	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	6	45	theme	hybridization	823:835	arg1	values					869:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	774:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T	774:922	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	4	46	theme	Substrate	480:488	arg1	mycelia					490:496	Substrate mycelia	480:496	Substrate mycelia	480:496	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
36268862	8	47	theme	JCM	1357:1359	arg1	31229T					1361:1366	S. colocasiea JCM 31229T	1343:1366	S. colocasiea JCM 31229T	1343:1366	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	2	48	theme	polyphasic	331:340	arg1	approach					342:349	a polyphasic approach	329:349	a polyphasic approach to determine its taxonomic position	329:385	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	5	49	theme	colocasiea	750:759	arg1	CC-MHH0539T					761:771	Sphingomonas colocasiea CC-MHH0539T	737:771	Sphingomonas colocasiea CC-MHH0539T	737:771	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	6	50	theme	digital	807:813	arg1	hybridization					823:835	digital DNA-DNA hybridization	807:835	digital DNA-DNA hybridization	807:835	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	10	51	theme	cell-wall	1444:1452	arg1	peptidoglycan					1454:1466	The cell-wall peptidoglycan	1440:1466	The cell-wall peptidoglycan	1440:1466	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	6	52	theme	identity	797:804	arg1	%					949:949	78.0, 21.7 and 70.8 %	929:949	%	949:949	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	6	52	theme	identity	797:804	arg1	values					869:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	774:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T	774:922	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	10	53	theme	meso-diaminopimelic	1489:1507	arg1	acid					1509:1512	meso-diaminopimelic acid	1489:1512	meso-diaminopimelic acid	1489:1512	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	5	54	theme	Sphingomonas	737:748	arg1	CC-MHH0539T					761:771	Sphingomonas colocasiea CC-MHH0539T	737:771	Sphingomonas colocasiea CC-MHH0539T	737:771	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	1	55	theme	novel	32:36	arg1	bacterium					49:57	a novel endophytic bacterium	30:57	a novel endophytic bacterium	30:57	nov., a novel endophytic bacterium isolated from Aegiceras corniculatum.
36268862	1	55	theme	novel	32:36	arg1	nov.					24:27	nov.	24:27	nov.	24:27	nov., a novel endophytic bacterium isolated from Aegiceras corniculatum.
36268862	2	56	dep	Gram-stain-negative	99:117	arg1	aerobic					120:126	aerobic	120:126	aerobic	120:126	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	2	56	dep	Gram-stain-negative	99:117	arg1	rod-shaped					137:146	rod-shaped	137:146	rod-shaped	137:146	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	2	56	dep	Gram-stain-negative	99:117	arg1	motile					129:134	motile	129:134	motile	129:134	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	14	57	theme	=JCM	2137:2140	arg1	CMS5P-6T					2127:2134	CMS5P-6T	2127:2134	CMS5P-6T (=JCM 33125T=CGMCC 1.13868T)	2127:2163	The type strain is CMS5P-6T (=JCM 33125T=CGMCC 1.13868T).
36268862	14	57	theme	=JCM	2137:2140	arg1	1.13868T					2155:2162	=JCM 33125T=CGMCC 1.13868T	2137:2162	=JCM 33125T=CGMCC 1.13868T	2137:2162	The type strain is CMS5P-6T (=JCM 33125T=CGMCC 1.13868T).
36268862	5	58	theme	Phylogenetic	596:607	arg1	analysis					609:616	Phylogenetic analysis	596:616	Phylogenetic analysis	596:616	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	6	59	theme	strain	884:889	arg1	CMS5P-6T					891:898	strain CMS5P-6T	884:898	strain CMS5P-6T	884:898	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	10	60	theme	diagnostic	1521:1530	arg1	lipoquinone					1599:1609	the respiratory lipoquinone	1583:1609	the respiratory lipoquinone	1583:1609	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	60	theme	diagnostic	1521:1530	arg1	acid					1540:1543	the diagnostic diamino acid	1517:1543	the diagnostic diamino acid	1517:1543	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	60	theme	diagnostic	1521:1530	arg1	Q-10					1560:1563	ubiquinone Q-10	1549:1563	ubiquinone Q-10	1549:1563	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	2	61	theme	Gram-stain-negative	99:117	arg1	bacterium					148:156	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium	97:156	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium	97:156	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	4	62	located	observed	566:573	arg1	media					582:586	the media	578:586	the media tested	578:593	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
36268862	4	62	located	observed	566:573	arg2	pigments					552:559	no diffusible pigments	538:559	no diffusible pigments	538:559	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
36268862	7	63	theme	identity	1052:1059	arg1	%					1146:1146	74.0, 19.9 and 61.4 %	1126:1146	%	1146:1146	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	7	63	theme	identity	1052:1059	arg1	values					1061:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	966:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T	966:1119	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	2	64	theme	Autonomous	280:289	arg1	Region					291:296	Guangxi Zhuang Autonomous Region	265:296	Guangxi Zhuang Autonomous Region	265:296	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	2	64	theme	Autonomous	280:289	arg1	China					302:306	PR China	299:306	PR China	299:306	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	9	65	theme	G+C	1377:1379	arg1	%					1437:1437	65.6 mol%	1429:1437	65.6 mol%	1429:1437	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	9	65	theme	G+C	1377:1379	arg1	content					1381:1387	The DNA G+C content	1369:1387	The DNA G+C content of strain CMS5P-6T	1369:1406	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	6	66	theme	acid	855:858	arg1	identity					860:867	average amino acid identity	841:867	average amino acid identity	841:867	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	10	67	theme	ubiquinone	1549:1558	arg1	lipoquinone					1599:1609	the respiratory lipoquinone	1583:1609	the respiratory lipoquinone	1583:1609	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	67	theme	ubiquinone	1549:1558	arg1	acid					1540:1543	the diagnostic diamino acid	1517:1543	the diagnostic diamino acid	1517:1543	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	67	theme	ubiquinone	1549:1558	arg1	Q-10					1560:1563	ubiquinone Q-10	1549:1563	ubiquinone Q-10	1549:1563	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	11	68	theme	polar	1616:1620	arg1	lipids					1622:1627	The polar lipids	1612:1627	The polar lipids	1612:1627	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	7	69	theme	amino	1041:1045	arg1	identity					1052:1059	average amino acid identity	1033:1059	average amino acid identity	1033:1059	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	8	70	theme	genome	1194:1199	arg1	sequences					1201:1209	genome sequences	1194:1209	genome sequences	1194:1209	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	11	71	theme	major	1805:1809	arg1	acids					1817:1821	the major fatty acids	1801:1821	the major fatty acids	1801:1821	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	11	71	theme	major	1805:1809	arg1	C16 					1876:1879	C16 	1876:1879	C16 	1876:1879	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	11	71	theme	major	1805:1809	arg1	C18 					1842:1845	C18 	1842:1845	C18 	1842:1845	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	5	72	theme	16S	658:660	arg1	similarity					681:690	high 16S rRNA gene sequence similarity	653:690	high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T	653:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	9	73	theme	CMS5P-6T	1399:1406	arg1	%					1437:1437	65.6 mol%	1429:1437	65.6 mol%	1429:1437	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	9	73	theme	CMS5P-6T	1399:1406	arg1	content					1381:1387	The DNA G+C content	1369:1387	The DNA G+C content of strain CMS5P-6T	1369:1406	The DNA G+C content of strain CMS5P-6T was determined to be 65.6 mol%.
36268862	6	74	theme	78.0	929:932	arg1	%					949:949	78.0, 21.7 and 70.8 %	929:949	%	949:949	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	6	74	theme	78.0	929:932	arg1	values					869:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	774:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T	774:922	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	12	75	theme	genus	2029:2033	arg1	Hephaestia					2035:2044	the genus Hephaestia	2025:2044	the genus Hephaestia	2025:2044	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	7	76	theme	DNA-DNA	1007:1013	arg1	hybridization					1015:1027	digital DNA-DNA hybridization	999:1027	digital DNA-DNA hybridization	999:1027	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	12	77	theme	name	2061:2064	arg1	sp					2086:2087	the name Hephaestia mangrovi sp	2057:2087	the name Hephaestia mangrovi sp	2057:2087	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	8	78	theme	strain	1223:1228	arg1	CMS5P-6T					1230:1237	strain CMS5P-6T	1223:1237	strain CMS5P-6T	1223:1237	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	5	79	theme	strain	630:635	arg1	CMS5P-6T					637:644	strain CMS5P-6T	630:644	strain CMS5P-6T	630:644	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	7	80	theme	average	970:976	arg1	identity					989:996	average nucleotide identity	970:996	average nucleotide identity	970:996	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	8	81	theme	Phylogenomic	1163:1174	arg1	analyses					1176:1183	Phylogenomic analyses	1163:1183	Phylogenomic analyses based on genome sequences	1163:1209	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	6	82	theme	caeni	907:911	arg1	25527T					917:922	H. caeni DSM 25527T	904:922	H. caeni DSM 25527T	904:922	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	12	83	theme	novel	2008:2012	arg1	species					2014:2020	a novel species	2006:2020	a novel species	2006:2020	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	7	84	theme	nucleotide	978:987	arg1	identity					989:996	average nucleotide identity	970:996	average nucleotide identity	970:996	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	8	85	dep	CMS5P-6T	1230:1237	arg1	25527T					1256:1261	DSM 25527T	1252:1261	DSM 25527T	1252:1261	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	5	86	theme	caeni	716:720	arg1	DSM					722:724	Hephaestia caeni DSM 25527T	705:731	Hephaestia caeni DSM 25527T	705:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	5	87	theme	rRNA	662:665	arg1	similarity					681:690	high 16S rRNA gene sequence similarity	653:690	high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T	653:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	1	88	theme	endophytic	38:47	arg1	bacterium					49:57	a novel endophytic bacterium	30:57	a novel endophytic bacterium	30:57	nov., a novel endophytic bacterium isolated from Aegiceras corniculatum.
36268862	1	88	theme	endophytic	38:47	arg1	nov.					24:27	nov.	24:27	nov.	24:27	nov., a novel endophytic bacterium isolated from Aegiceras corniculatum.
36268862	6	89	theme	70.8 	944:948	arg1	%					949:949	78.0, 21.7 and 70.8 %	929:949	%	949:949	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	6	89	theme	70.8 	944:948	arg1	values					869:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	774:874	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T	774:922	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	10	90	contain	contain	1481:1487	arg1	peptidoglycan					1454:1466	The cell-wall peptidoglycan	1440:1466	The cell-wall peptidoglycan	1440:1466	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	10	90	contain	contain	1481:1487	arg2	acid					1509:1512	meso-diaminopimelic acid	1489:1512	meso-diaminopimelic acid	1489:1512	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid as the diagnostic diamino acid and ubiquinone Q-10 was identified as the respiratory lipoquinone.
36268862	3	91	theme	0-1 	437:440	arg1	%					441:441	%	441:441	%	441:441	Strain CMS5P-6T was found to grow optimally with 0-1 % (w/v) NaCl, at 30 °C and pH 6.0-7.0.
36268862	0	92	theme	mangrovi	11:18	arg1	sp					20:21	Hephaestia mangrovi sp	0:21	Hephaestia mangrovi sp.	0:22	Hephaestia mangrovi sp.
36268862	11	93	theme	 0	1860:1861	arg1	cycloω8c					1863:1870	 0 cycloω8c	1860:1870	C18 : 1 ω7c, C19 : 0 cycloω8c	1842:1870	The polar lipids were found to comprise diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, sphingoglycolipid and two unidentified aminolipids, and the major fatty acids were identified as C18 : 1 ω7c, C19 : 0 cycloω8c and C16 : 0.
36268862	1	94	theme	Aegiceras	73:81	arg1	corniculatum					83:94	Aegiceras corniculatum	73:94	Aegiceras corniculatum	73:94	nov., a novel endophytic bacterium isolated from Aegiceras corniculatum.
36268862	3	95	theme	Strain	388:393	arg1	CMS5P-6T					395:402	Strain CMS5P-6T	388:402	Strain CMS5P-6T	388:402	Strain CMS5P-6T was found to grow optimally with 0-1 % (w/v) NaCl, at 30 °C and pH 6.0-7.0.
36268862	2	96	theme	taxonomic	368:376	arg1	position					378:385	its taxonomic position	364:385	its taxonomic position	364:385	A Gram-stain-negative, aerobic, motile, rod-shaped bacterium, designated CMS5P-6T, was isolated from a surface-sterilized bark of Aegiceras corniculatum collected from Guangxi Zhuang Autonomous Region, PR China, and investigated by a polyphasic approach to determine its taxonomic position.
36268862	12	97	dep	data	1954:1957	arg1	the					1888:1890	the	1888:1890	the	1888:1890	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	12	97	dep	data	1954:1957	arg1	basis					1892:1896	basis	1892:1896	basis	1892:1896	On the basis of phylogenetic, genomic, chemotaxonomic and phenotypic data, strain CMS5P-6T can be concluded to represent a novel species of the genus Hephaestia, for which the name Hephaestia mangrovi sp.
36268862	7	98	theme	JCM	1110:1112	arg1	31229T					1114:1119	S. colocasiea JCM 31229T	1096:1119	S. colocasiea JCM 31229T	1096:1119	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	7	99	theme	61.4 	1141:1145	arg1	%					1146:1146	74.0, 19.9 and 61.4 %	1126:1146	%	1146:1146	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	7	99	theme	61.4 	1141:1145	arg1	values					1061:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values	966:1066	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T	966:1119	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	7	100	theme	S.	1096:1097	arg1	31229T					1114:1119	S. colocasiea JCM 31229T	1096:1119	S. colocasiea JCM 31229T	1096:1119	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and S. colocasiea JCM 31229T were 74.0, 19.9 and 61.4 %, respectively.
36268862	6	101	theme	DNA-DNA	815:821	arg1	hybridization					823:835	digital DNA-DNA hybridization	807:835	digital DNA-DNA hybridization	807:835	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	5	102	theme	sequence	672:679	arg1	similarity					681:690	high 16S rRNA gene sequence similarity	653:690	high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T	653:731	Phylogenetic analysis showed that strain CMS5P-6T showed high 16S rRNA gene sequence similarity of 96.7 % to Hephaestia caeni DSM 25527T and Sphingomonas colocasiea CC-MHH0539T.
36268862	4	103	theme	aerial	502:507	arg1	mycelia					509:515	aerial mycelia	502:515	aerial mycelia	502:515	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
36268862	8	104	theme	colocasiea	1346:1355	arg1	31229T					1361:1366	S. colocasiea JCM 31229T	1343:1366	S. colocasiea JCM 31229T	1343:1366	Phylogenomic analyses based on genome sequences showed that strain CMS5P-6T and H. caeni DSM 25527T formed a distinct cluster within the family Sphingomonadaceae and far away from S. colocasiea JCM 31229T.
36268862	3	105	dep	%	441:441	arg1	w/v					444:446	w/v	444:446	w/v	444:446	Strain CMS5P-6T was found to grow optimally with 0-1 % (w/v) NaCl, at 30 °C and pH 6.0-7.0.
36268862	6	106	theme	nucleotide	786:795	arg1	identity					797:804	average nucleotide identity	778:804	average nucleotide identity	778:804	The average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity values between strain CMS5P-6T and H. caeni DSM 25527T were 78.0, 21.7 and 70.8 %, respectively.
36268862	14	107	theme	33125T=CGMCC	2142:2153	arg1	CMS5P-6T					2127:2134	CMS5P-6T	2127:2134	CMS5P-6T (=JCM 33125T=CGMCC 1.13868T)	2127:2163	The type strain is CMS5P-6T (=JCM 33125T=CGMCC 1.13868T).
36268862	14	107	theme	33125T=CGMCC	2142:2153	arg1	1.13868T					2155:2162	=JCM 33125T=CGMCC 1.13868T	2137:2162	=JCM 33125T=CGMCC 1.13868T	2137:2162	The type strain is CMS5P-6T (=JCM 33125T=CGMCC 1.13868T).
35889506	5	0	dep	levels	756:761	arg1	cyclooxygenase					763:776	cyclooxygenase (COX)-2	763:784	cyclooxygenase (COX)-2	763:784	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	0	dep	levels	756:761	arg1	COX					779:781	COX	779:781	COX	779:781	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	0	dep	levels	756:761	arg1	-α					856:857	tumor necrosis factor (TNF)-α	829:857	tumor necrosis factor (TNF)-α	829:857	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	0	dep	levels	756:761	arg1	synthase					810:817	inducible nitric oxide synthase (iNOS) 2	787:826	inducible nitric oxide synthase (iNOS) 2	787:826	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	0	dep	levels	756:761	arg1	interleukin					864:874	interleukin (IL)-6	864:881	interleukin (IL)-6	864:881	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	0	dep	levels	756:761	arg1	iNOS					820:823	iNOS	820:823	iNOS	820:823	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	0	dep	levels	756:761	arg1	levels					756:761	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	0	dep	levels	756:761	arg1	IL					877:878	IL	877:878	IL	877:878	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	1	theme	qRT-PCR	689:695	arg1	results					697:703	The qRT-PCR results	685:703	The qRT-PCR results	685:703	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	6	2	theme	TNF-α	983:987	arg1	production					955:964	the production	951:964	the production of nitric oxide, TNF-α, and IL-6	951:997	The FGE treatment also decreased the production of nitric oxide, TNF-α, and IL-6 significantly in a dose-dependent manner.
35889506	7	3	theme	signaling	1106:1114	arg1	proteins					1132:1139	NF-κB signaling pathway-related proteins	1100:1139	NF-κB signaling pathway-related proteins	1100:1139	In addition, FGE downregulated phosphorylation of MAPK and NF-κB signaling pathway-related proteins.
35889506	8	4	theme	mass	1166:1169	arg1	results					1184:1190	The chromatographic and mass spectrometry results	1142:1190	The chromatographic and mass spectrometry results	1142:1190	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	4	5	theme	ethanol	494:500	arg1	extract					502:508	The ethanol extract	490:508	The ethanol extract of Filipendula glaberrima Nakai (FGE)	490:546	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	4	6	theme	power	618:622	arg1	assays					624:629	reducing power assays	609:629	reducing power assays	609:629	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	7	7	theme	MAPK	1091:1094	arg1	phosphorylation					1072:1086	phosphorylation	1072:1086	phosphorylation of MAPK and NF-κB signaling pathway-related proteins	1072:1139	In addition, FGE downregulated phosphorylation of MAPK and NF-κB signaling pathway-related proteins.
35889506	2	8	theme	Nakai	325:329	arg1	effects					291:297	the effects	287:297	the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity	287:384	This study examined the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system.
35889506	6	9	theme	oxide	976:980	arg1	production					955:964	the production	951:964	the production of nitric oxide, TNF-α, and IL-6	951:997	The FGE treatment also decreased the production of nitric oxide, TNF-α, and IL-6 significantly in a dose-dependent manner.
35889506	0	10	theme	Chemical	101:108	arg1	Composition					110:120	Its Chemical Composition	97:120	Its Chemical Composition	97:120	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract and Its Chemical Composition.
35889506	9	11	theme	anti-inflammatory	1531:1547	arg1	agent					1549:1553	a novel natural anti-inflammatory agent	1515:1553	a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids	1515:1595	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	8	12	theme	active	1312:1317	arg1	compounds					1319:1327	active compounds	1312:1327	active compounds with antioxidant and anti-inflammatory activities	1312:1377	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	9	13	theme	anti-inflammatory	1424:1440	arg1	activity					1442:1449	the anti-inflammatory activity	1420:1449	the anti-inflammatory activity of the FG	1420:1459	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	6	14	theme	nitric	969:974	arg1	oxide					976:980	nitric oxide	969:980	nitric oxide	969:980	The FGE treatment also decreased the production of nitric oxide, TNF-α, and IL-6 significantly in a dose-dependent manner.
35889506	8	15	theme	spectrometry	1171:1182	arg1	results					1184:1190	The chromatographic and mass spectrometry results	1142:1190	The chromatographic and mass spectrometry results	1142:1190	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	5	16	theme	RAW	901:903	arg1	cells					911:915	LPS-stimulated RAW 264.7 cells	886:915	LPS-stimulated RAW 264.7 cells	886:915	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	6	17	theme	dose-dependent	1018:1031	arg1	manner					1033:1038	a dose-dependent manner	1016:1038	a dose-dependent manner	1016:1038	The FGE treatment also decreased the production of nitric oxide, TNF-α, and IL-6 significantly in a dose-dependent manner.
35889506	2	18	dep	in	395:396	arg1	vitro					398:402	vitro	398:402	vitro	398:402	This study examined the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system.
35889506	3	19	theme	chromatographic	462:476	arg1	techniques					478:487	chromatographic techniques	462:487	chromatographic techniques	462:487	The bioactive components were also examined using chromatographic techniques.
35889506	5	20	theme	inducible	787:795	arg1	iNOS					820:823	iNOS	820:823	iNOS	820:823	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	20	theme	inducible	787:795	arg1	synthase					810:817	inducible nitric oxide synthase (iNOS) 2	787:826	inducible nitric oxide synthase (iNOS) 2	787:826	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	20	theme	inducible	787:795	arg1	levels					756:761	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	21	theme	LPS-stimulated	886:899	arg1	cells					911:915	LPS-stimulated RAW 264.7 cells	886:915	LPS-stimulated RAW 264.7 cells	886:915	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	22	theme	necrosis	835:842	arg1	TNF					852:854	TNF	852:854	TNF	852:854	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	22	theme	necrosis	835:842	arg1	factor					844:849	tumor necrosis factor	829:849	tumor necrosis factor (TNF)-α	829:857	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	1	23	theme	biological	197:206	arg1	resources					208:216	their biological resources	191:216	their biological resources	191:216	Many countries are endeavoring to strengthen the competitiveness of their biological resources by exploring and developing wild endemic plants.
35889506	5	24	theme	tumor	829:833	arg1	TNF					852:854	TNF	852:854	TNF	852:854	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	24	theme	tumor	829:833	arg1	factor					844:849	tumor necrosis factor	829:849	tumor necrosis factor (TNF)-α	829:857	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	25	theme	nitric	797:802	arg1	iNOS					820:823	iNOS	820:823	iNOS	820:823	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	25	theme	nitric	797:802	arg1	synthase					810:817	inducible nitric oxide synthase (iNOS) 2	787:826	inducible nitric oxide synthase (iNOS) 2	787:826	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	25	theme	nitric	797:802	arg1	levels					756:761	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	8	26	theme	anti-inflammatory	1350:1366	arg1	activities					1368:1377	antioxidant and anti-inflammatory activities	1334:1377	antioxidant and anti-inflammatory activities	1334:1377	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	1	27	theme	resources	208:216	arg1	competitiveness					172:186	the competitiveness	168:186	the competitiveness of their biological resources	168:216	Many countries are endeavoring to strengthen the competitiveness of their biological resources by exploring and developing wild endemic plants.
35889506	0	28	theme	Antioxidant	0:10	arg1	Activity					34:41	Antioxidant and Anti-Inflammatory Activity	0:41	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract	0:91	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract and Its Chemical Composition.
35889506	9	29	theme	bioactive	1576:1584	arg1	flavonoids					1586:1595	FGE-containing bioactive flavonoids	1561:1595	FGE-containing bioactive flavonoids	1561:1595	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	9	30	theme	FG	1458:1459	arg1	activity					1442:1449	the anti-inflammatory activity	1420:1449	the anti-inflammatory activity of the FG	1420:1459	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	5	31	theme	factor	844:849	arg1	-α					856:857	tumor necrosis factor (TNF)-α	829:857	tumor necrosis factor (TNF)-α	829:857	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	4	32	theme	radical	586:592	arg1	scavenging					594:603	the radical scavenging	582:603	the radical scavenging	582:603	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	7	33	theme	NF-κB	1100:1104	arg1	proteins					1132:1139	NF-κB signaling pathway-related proteins	1100:1139	NF-κB signaling pathway-related proteins	1100:1139	In addition, FGE downregulated phosphorylation of MAPK and NF-κB signaling pathway-related proteins.
35889506	4	34	theme	compounds	674:682	arg1	amounts					644:650	high amounts	639:650	high amounts of total polyphenolic compounds	639:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	4	34	theme	compounds	674:682	arg1	compounds					674:682	total polyphenolic compounds	655:682	total polyphenolic compounds	655:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	0	35	theme	Anti-Inflammatory	16:32	arg1	Activity					34:41	Antioxidant and Anti-Inflammatory Activity	0:41	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract	0:91	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract and Its Chemical Composition.
35889506	4	36	theme	reducing	609:616	arg1	assays					624:629	reducing power assays	609:629	reducing power assays	609:629	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	8	37	theme	antioxidant	1334:1344	arg1	activities					1368:1377	antioxidant and anti-inflammatory activities	1334:1377	antioxidant and anti-inflammatory activities	1334:1377	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	1	38	theme	Many	123:126	arg1	countries					128:136	Many countries	123:136	Many countries	123:136	Many countries are endeavoring to strengthen the competitiveness of their biological resources by exploring and developing wild endemic plants.
35889506	2	39	from	effects	291:297	arg1	activity					377:384	the antioxidant and anti-inflammatory activity	339:384	the antioxidant and anti-inflammatory activity	339:384	This study examined the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system.
35889506	4	40	theme	polyphenolic	661:672	arg1	compounds					674:682	total polyphenolic compounds	655:682	total polyphenolic compounds	655:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	8	41	theme	bioactive	1218:1226	arg1	miquelianin					1261:1271	miquelianin	1261:1271	miquelianin	1261:1271	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	41	theme	bioactive	1218:1226	arg1	quercitrin					1274:1283	quercitrin	1274:1283	quercitrin	1274:1283	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	41	theme	bioactive	1218:1226	arg1	afzelin					1290:1296	afzelin	1290:1296	afzelin	1290:1296	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	41	theme	bioactive	1218:1226	arg1	flavonoids					1228:1237	bioactive flavonoids	1218:1237	bioactive flavonoids	1218:1237	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	41	theme	bioactive	1218:1226	arg1	-catechin					1250:1258	-catechin	1250:1258	-catechin	1250:1258	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	9	42	theme	FGE-containing	1561:1574	arg1	flavonoids					1586:1595	FGE-containing bioactive flavonoids	1561:1595	FGE-containing bioactive flavonoids	1561:1595	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	9	43	from	data	1412:1415	arg1	activity					1442:1449	the anti-inflammatory activity	1420:1449	the anti-inflammatory activity of the FG	1420:1459	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	7	44	theme	pathway-related	1116:1130	arg1	proteins					1132:1139	NF-κB signaling pathway-related proteins	1100:1139	NF-κB signaling pathway-related proteins	1100:1139	In addition, FGE downregulated phosphorylation of MAPK and NF-κB signaling pathway-related proteins.
35889506	4	45	contain	had	635:637	arg2	amounts					644:650	high amounts	639:650	high amounts of total polyphenolic compounds	639:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	4	45	contain	had	635:637	arg2	compounds					674:682	total polyphenolic compounds	655:682	total polyphenolic compounds	655:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	4	45	contain	had	635:637	arg1	extract					502:508	The ethanol extract	490:508	The ethanol extract of Filipendula glaberrima Nakai (FGE)	490:546	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	1	46	theme	wild	246:249	arg1	plants					259:264	wild endemic plants	246:264	wild endemic plants	246:264	Many countries are endeavoring to strengthen the competitiveness of their biological resources by exploring and developing wild endemic plants.
35889506	3	47	theme	bioactive	416:424	arg1	components					426:435	The bioactive components	412:435	The bioactive components	412:435	The bioactive components were also examined using chromatographic techniques.
35889506	4	48	theme	antioxidant	556:566	arg1	activities					568:577	antioxidant activities	556:577	antioxidant activities	556:577	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	0	49	theme	Ethanolic	75:83	arg1	Extract					85:91	Filipendula glaberrima Nakai Ethanolic Extract	46:91	Filipendula glaberrima Nakai Ethanolic Extract	46:91	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract and Its Chemical Composition.
35889506	8	50	contain	contained	1208:1216	arg2	quercitrin					1274:1283	quercitrin	1274:1283	quercitrin	1274:1283	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	50	contain	contained	1208:1216	arg2	flavonoids					1228:1237	bioactive flavonoids	1218:1237	bioactive flavonoids	1218:1237	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	50	contain	contained	1208:1216	arg2	afzelin					1290:1296	afzelin	1290:1296	afzelin	1290:1296	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	50	contain	contained	1208:1216	arg2	-catechin					1250:1258	-catechin	1250:1258	-catechin	1250:1258	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	50	contain	contained	1208:1216	arg2	miquelianin					1261:1271	miquelianin	1261:1271	miquelianin	1261:1271	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	8	50	contain	contained	1208:1216	arg1	FGE					1204:1206	FGE	1204:1206	FGE	1204:1206	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	9	51	theme	novel	1517:1521	arg1	agent					1549:1553	a novel natural anti-inflammatory agent	1515:1553	a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids	1515:1595	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	2	52	theme	anti-inflammatory	359:375	arg1	activity					377:384	the antioxidant and anti-inflammatory activity	339:384	the antioxidant and anti-inflammatory activity	339:384	This study examined the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system.
35889506	7	53	theme	proteins	1132:1139	arg1	phosphorylation					1072:1086	phosphorylation	1072:1086	phosphorylation of MAPK and NF-κB signaling pathway-related proteins	1072:1139	In addition, FGE downregulated phosphorylation of MAPK and NF-κB signaling pathway-related proteins.
35889506	6	54	theme	FGE	922:924	arg1	treatment					926:934	The FGE treatment	918:934	The FGE treatment	918:934	The FGE treatment also decreased the production of nitric oxide, TNF-α, and IL-6 significantly in a dose-dependent manner.
35889506	9	55	theme	starting	1485:1492	arg1	point					1494:1498	a good starting point	1478:1498	a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids	1478:1595	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	9	55	theme	starting	1485:1492	arg1	study					1385:1389	This study	1380:1389	This study	1380:1389	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	9	56	theme	good	1480:1483	arg1	point					1494:1498	a good starting point	1478:1498	a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids	1478:1595	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	9	56	theme	good	1480:1483	arg1	study					1385:1389	This study	1380:1389	This study	1380:1389	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	6	57	theme	IL-6	994:997	arg1	production					955:964	the production	951:964	the production of nitric oxide, TNF-α, and IL-6	951:997	The FGE treatment also decreased the production of nitric oxide, TNF-α, and IL-6 significantly in a dose-dependent manner.
35889506	5	58	theme	oxide	804:808	arg1	iNOS					820:823	iNOS	820:823	iNOS	820:823	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	58	theme	oxide	804:808	arg1	synthase					810:817	inducible nitric oxide synthase (iNOS) 2	787:826	inducible nitric oxide synthase (iNOS) 2	787:826	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	5	58	theme	oxide	804:808	arg1	levels					756:761	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6	752:881	The qRT-PCR results suggested that FGE significantly downregulated the levels cyclooxygenase (COX)-2, inducible nitric oxide synthase (iNOS) 2, tumor necrosis factor (TNF)-α, and interleukin (IL)-6 in LPS-stimulated RAW 264.7 cells.
35889506	2	59	theme	antioxidant	343:353	arg1	activity					377:384	the antioxidant and anti-inflammatory activity	339:384	the antioxidant and anti-inflammatory activity	339:384	This study examined the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system.
35889506	8	60	with	compounds	1319:1327	arg1	activities					1368:1377	antioxidant and anti-inflammatory activities	1334:1377	antioxidant and anti-inflammatory activities	1334:1377	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	1	61	theme	endemic	251:257	arg1	plants					259:264	wild endemic plants	246:264	wild endemic plants	246:264	Many countries are endeavoring to strengthen the competitiveness of their biological resources by exploring and developing wild endemic plants.
35889506	8	62	dep	-catechin	1250:1258	arg1	+					1248:1248	+	1248:1248	+	1248:1248	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
35889506	9	63	theme	natural	1523:1529	arg1	agent					1549:1553	a novel natural anti-inflammatory agent	1515:1553	a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids	1515:1595	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	4	64	theme	high	639:642	arg1	amounts					644:650	high amounts	639:650	high amounts of total polyphenolic compounds	639:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	4	64	theme	high	639:642	arg1	compounds					674:682	total polyphenolic compounds	655:682	total polyphenolic compounds	655:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	0	65	theme	Extract	85:91	arg1	Composition					110:120	Its Chemical Composition	97:120	Its Chemical Composition	97:120	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract and Its Chemical Composition.
35889506	0	65	theme	Extract	85:91	arg1	Activity					34:41	Antioxidant and Anti-Inflammatory Activity	0:41	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract	0:91	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract and Its Chemical Composition.
35889506	2	66	theme	in	395:396	arg1	system					404:409	an in vitro system	392:409	an in vitro system	392:409	This study examined the effects of Filipendula glaberrima Nakai (FG) on the antioxidant and anti-inflammatory activity using an in vitro system.
35889506	4	67	theme	total	655:659	arg1	compounds					674:682	total polyphenolic compounds	655:682	total polyphenolic compounds	655:682	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	0	68	theme	Nakai	69:73	arg1	Extract					85:91	Filipendula glaberrima Nakai Ethanolic Extract	46:91	Filipendula glaberrima Nakai Ethanolic Extract	46:91	Antioxidant and Anti-Inflammatory Activity of Filipendula glaberrima Nakai Ethanolic Extract and Its Chemical Composition.
35889506	4	69	theme	FGE	543:545	arg1	extract					502:508	The ethanol extract	490:508	The ethanol extract of Filipendula glaberrima Nakai (FGE)	490:546	The ethanol extract of Filipendula glaberrima Nakai (FGE) exerted antioxidant activities in the radical scavenging and reducing power assays and had high amounts of total polyphenolic compounds.
35889506	9	70	theme	fundamental	1400:1410	arg1	data					1412:1415	fundamental data	1400:1415	fundamental data on the anti-inflammatory activity of the FG	1400:1459	This study provides fundamental data on the anti-inflammatory activity of the FG and can serve as a good starting point for developing a novel natural anti-inflammatory agent using FGE-containing bioactive flavonoids.
35889506	8	71	theme	chromatographic	1146:1160	arg1	results					1184:1190	The chromatographic and mass spectrometry results	1142:1190	The chromatographic and mass spectrometry results	1142:1190	The chromatographic and mass spectrometry results showed that FGE contained bioactive flavonoids such as (+)-catechin, miquelianin, quercitrin, and afzelin, which may be active compounds with antioxidant and anti-inflammatory activities.
36525001	2	0	from	secretion	243:251	arg1	macrophages					256:266	macrophages	256:266	macrophages	256:266	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	7	1	theme	ATP-induced	1198:1208	arg1	vesiculation					1210:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	Our data demonstrate that this process differs significantly from canonical inflammasome activator ATP-induced vesiculation, and it is dependent on the autocrine IFN signal associated with TLR4 activation.
36525001	1	2	theme	immunoregulatory	191:206	arg1	proteins					208:215	numerous immunoregulatory proteins	182:215	numerous immunoregulatory proteins	182:215	Human macrophages secrete extracellular vesicles (EVs) loaded with numerous immunoregulatory proteins.
36525001	5	3	theme	LPS	886:888	arg1	delivery					870:877	delivery	870:877	delivery of the LPS with Escherichia coli-derived outer membrane vesicles	870:942	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	7	4	theme	TLR4	1288:1291	arg1	activation					1293:1302	TLR4 activation	1288:1302	TLR4 activation	1288:1302	Our data demonstrate that this process differs significantly from canonical inflammasome activator ATP-induced vesiculation, and it is dependent on the autocrine IFN signal associated with TLR4 activation.
36525001	8	5	theme	inflammasome	1421:1432	arg1	ASC					1456:1458	ASC	1456:1458	ASC	1456:1458	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	5	theme	inflammasome	1421:1432	arg1	caspase-1					1445:1453	caspase-1	1445:1453	caspase-1	1445:1453	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	5	theme	inflammasome	1421:1432	arg1	components					1434:1443	inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1	1421:1513	inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1	1421:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	3	6	theme	caspase-4/5	608:618	arg1	activation					633:642	noncanonical caspase-4/5 inflammasome activation	595:642	noncanonical caspase-4/5 inflammasome activation	595:642	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	5	7	theme	similar	843:849	arg1	effect					851:856	A similar effect	841:856	A similar effect	841:856	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	6	8	theme	LPS	1025:1027	arg1	transfection					1029:1040	LPS transfection	1025:1040	LPS transfection	1025:1040	Moreover, sensitization of the macrophages through TLR4 by LPS priming prior to LPS transfection dramatically augments the EV-mediated protein secretion.
36525001	2	9	theme	protein	415:421	arg1	composition					423:433	protein composition	415:433	protein composition	415:433	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	5	10	theme	coli-derived	907:918	arg1	vesicles					935:942	Escherichia coli-derived outer membrane vesicles	895:942	Escherichia coli-derived outer membrane vesicles	895:942	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	2	11	dep	quantities	400:409	arg1	the					396:398	the	396:398	the	396:398	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	4	12	theme	caspase-4-dependent	713:731	arg1	secretion					736:744	robust caspase-4-dependent EV secretion	706:744	robust caspase-4-dependent EV secretion	706:744	We show that human macrophages activate robust caspase-4-dependent EV secretion upon transfection of LPS, and this process is also partially dependent on NLRP3 and caspase-5.
36525001	9	13	theme	caspase-4-mediated	1712:1729	arg1	inflammation					1731:1742	caspase-4-mediated inflammation	1712:1742	caspase-4-mediated inflammation	1712:1742	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	8	14	theme	inflammatory	1532:1543	arg1	transfer					1548:1555	inflammatory EV transfer	1532:1555	inflammatory EV transfer	1532:1555	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	3	15	theme	noncanonical	595:606	arg1	activation					633:642	noncanonical caspase-4/5 inflammasome activation	595:642	noncanonical caspase-4/5 inflammasome activation	595:642	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	4	16	theme	robust	706:711	arg1	secretion					736:744	robust caspase-4-dependent EV secretion	706:744	robust caspase-4-dependent EV secretion	706:744	We show that human macrophages activate robust caspase-4-dependent EV secretion upon transfection of LPS, and this process is also partially dependent on NLRP3 and caspase-5.
36525001	8	17	theme	inflammasome	1344:1355	arg1	activation					1357:1366	the noncanonical inflammasome activation	1327:1366	the noncanonical inflammasome activation	1327:1366	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	6	18	theme	prior	1016:1020	arg1	priming					1008:1014	LPS priming	1004:1014	LPS priming prior to LPS transfection	1004:1040	Moreover, sensitization of the macrophages through TLR4 by LPS priming prior to LPS transfection dramatically augments the EV-mediated protein secretion.
36525001	5	19	dep	coli-derived	907:918	arg1	Escherichia					895:905	Escherichia	895:905	Escherichia	895:905	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	8	20	theme	EV	1545:1546	arg1	transfer					1548:1555	inflammatory EV transfer	1532:1555	inflammatory EV transfer	1532:1555	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	9	21	theme	bioinformatics	1621:1634	arg1	methods					1636:1642	bioinformatics methods	1621:1642	bioinformatics methods	1621:1642	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	5	22	theme	outer	920:924	arg1	vesicles					935:942	Escherichia coli-derived outer membrane vesicles	895:942	Escherichia coli-derived outer membrane vesicles	895:942	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	7	23	theme	IFN	1261:1263	arg1	signal					1265:1270	the autocrine IFN signal	1247:1270	the autocrine IFN signal associated with TLR4 activation	1247:1302	Our data demonstrate that this process differs significantly from canonical inflammasome activator ATP-induced vesiculation, and it is dependent on the autocrine IFN signal associated with TLR4 activation.
36525001	9	24	theme	inflammation	1731:1742	arg1	15-deoxy-Δ12,14-PGJ2					1657:1676	15-deoxy-Δ12,14-PGJ2	1657:1676	15-deoxy-Δ12,14-PGJ2	1657:1676	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	9	24	theme	inflammation	1731:1742	arg1	inhibitors					1698:1707	inhibitors	1698:1707	inhibitors of caspase-4-mediated inflammation and vesicle secretion	1698:1764	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	9	24	theme	inflammation	1731:1742	arg1	parthenolide					1682:1693	parthenolide	1682:1693	parthenolide	1682:1693	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	1	25	theme	Human	115:119	arg1	macrophages					121:131	Human macrophages	115:131	Human macrophages	115:131	Human macrophages secrete extracellular vesicles (EVs) loaded with numerous immunoregulatory proteins.
36525001	5	26	theme	membrane	926:933	arg1	vesicles					935:942	Escherichia coli-derived outer membrane vesicles	895:942	Escherichia coli-derived outer membrane vesicles	895:942	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	6	27	theme	LPS	1004:1006	arg1	priming					1008:1014	LPS priming	1004:1014	LPS priming prior to LPS transfection	1004:1040	Moreover, sensitization of the macrophages through TLR4 by LPS priming prior to LPS transfection dramatically augments the EV-mediated protein secretion.
36525001	9	28	theme	vesicle	1748:1754	arg1	secretion					1756:1764	vesicle secretion	1748:1764	vesicle secretion	1748:1764	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	9	29	theme	therapeutic	1782:1792	arg1	potential					1794:1802	new therapeutic potential	1778:1802	new therapeutic potential of these anti-inflammatory drugs	1778:1835	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	3	30	theme	high-throughput	484:498	arg1	proteomics					513:522	high-throughput quantitative proteomics	484:522	high-throughput quantitative proteomics	484:522	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	7	31	theme	autocrine	1251:1259	arg1	signal					1265:1270	the autocrine IFN signal	1247:1270	the autocrine IFN signal associated with TLR4 activation	1247:1302	Our data demonstrate that this process differs significantly from canonical inflammasome activator ATP-induced vesiculation, and it is dependent on the autocrine IFN signal associated with TLR4 activation.
36525001	9	32	theme	secretion	1756:1764	arg1	15-deoxy-Δ12,14-PGJ2					1657:1676	15-deoxy-Δ12,14-PGJ2	1657:1676	15-deoxy-Δ12,14-PGJ2	1657:1676	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	9	32	theme	secretion	1756:1764	arg1	inhibitors					1698:1707	inhibitors	1698:1707	inhibitors of caspase-4-mediated inflammation and vesicle secretion	1698:1764	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	9	32	theme	secretion	1756:1764	arg1	parthenolide					1682:1693	parthenolide	1682:1693	parthenolide	1682:1693	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	2	33	theme	vesicles	447:454	arg1	quantities					400:409	quantities	400:409	quantities	400:409	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	2	33	theme	vesicles	447:454	arg1	composition					423:433	protein composition	415:433	protein composition	415:433	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	8	34	theme	vesicle-mediated	1391:1406	arg1	secretion					1408:1416	vesicle-mediated secretion	1391:1416	vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1	1391:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	35	theme	noncanonical	1331:1342	arg1	activation					1357:1366	the noncanonical inflammasome activation	1327:1366	the noncanonical inflammasome activation	1327:1366	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	36	dep	components	1434:1443	arg1	caspase-1					1445:1453	caspase-1	1445:1453	caspase-1	1445:1453	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	36	dep	components	1434:1443	arg1	NINJ1					1509:1513	NINJ1	1509:1513	NINJ1	1509:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	36	dep	components	1434:1443	arg1	ASC					1456:1458	ASC	1456:1458	ASC	1456:1458	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	36	dep	components	1434:1443	arg1	effectors					1482:1490	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	36	dep	components	1434:1443	arg1	GSDMD					1492:1496	GSDMD	1492:1496	GSDMD	1492:1496	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	36	dep	components	1434:1443	arg1	MLKL					1499:1502	MLKL	1499:1502	MLKL	1499:1502	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	36	dep	components	1434:1443	arg1	components					1434:1443	inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1	1421:1513	inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1	1421:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	1	37	theme	extracellular	141:153	arg1	EVs					165:167	EVs	165:167	EVs	165:167	Human macrophages secrete extracellular vesicles (EVs) loaded with numerous immunoregulatory proteins.
36525001	1	37	theme	extracellular	141:153	arg1	vesicles					155:162	extracellular vesicles	141:162	extracellular vesicles (EVs) loaded with numerous immunoregulatory proteins	141:215	Human macrophages secrete extracellular vesicles (EVs) loaded with numerous immunoregulatory proteins.
36525001	9	38	theme	drugs	1831:1835	arg1	potential					1794:1802	new therapeutic potential	1778:1802	new therapeutic potential of these anti-inflammatory drugs	1778:1835	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	4	39	theme	LPS	767:769	arg1	transfection					751:762	transfection	751:762	transfection of LPS	751:769	We show that human macrophages activate robust caspase-4-dependent EV secretion upon transfection of LPS, and this process is also partially dependent on NLRP3 and caspase-5.
36525001	0	40	theme	Human	26:30	arg1	Macrophages					32:42	Human Macrophages	26:42	Human Macrophages	26:42	Lipopolysaccharide Primes Human Macrophages for Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion.
36525001	4	41	theme	human	679:683	arg1	macrophages					685:695	human macrophages	679:695	human macrophages	679:695	We show that human macrophages activate robust caspase-4-dependent EV secretion upon transfection of LPS, and this process is also partially dependent on NLRP3 and caspase-5.
36525001	9	42	theme	new	1778:1780	arg1	potential					1794:1802	new therapeutic potential	1778:1802	new therapeutic potential of these anti-inflammatory drugs	1778:1835	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	2	43	theme	inflammatory	347:358	arg1	conditions					360:369	inflammatory conditions	347:369	inflammatory conditions	347:369	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	8	44	theme	LPS	1305:1307	arg1	priming					1309:1315	LPS priming	1305:1315	LPS priming preceding the noncanonical inflammasome activation	1305:1366	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	2	45	theme	secreted	438:445	arg1	vesicles					447:454	secreted vesicles	438:454	secreted vesicles	438:454	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	0	46	theme	Noncanonical	48:59	arg1	Secretion					104:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Lipopolysaccharide Primes Human Macrophages for Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion.
36525001	2	47	theme	protein	235:241	arg1	secretion					243:251	Vesicle-mediated protein secretion	218:251	Vesicle-mediated protein secretion in macrophages	218:266	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	5	48	link	coli-derived	907:918	arg1	vesicles					935:942	Escherichia coli-derived outer membrane vesicles	895:942	Escherichia coli-derived outer membrane vesicles	895:942	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	3	49	theme	quantitative	500:511	arg1	proteomics					513:522	high-throughput quantitative proteomics	484:522	high-throughput quantitative proteomics	484:522	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	8	50	theme	death	1476:1480	arg1	effectors					1482:1490	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	50	theme	death	1476:1480	arg1	MLKL					1499:1502	MLKL	1499:1502	MLKL	1499:1502	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	50	theme	death	1476:1480	arg1	NINJ1					1509:1513	NINJ1	1509:1513	NINJ1	1509:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	50	theme	death	1476:1480	arg1	GSDMD					1492:1496	GSDMD	1492:1496	GSDMD	1492:1496	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	2	51	theme	Vesicle-mediated	218:233	arg1	secretion					243:251	Vesicle-mediated protein secretion	218:251	Vesicle-mediated protein secretion in macrophages	218:266	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	2	52	theme	characterized	291:303	arg1	mechanisms					305:314	poorly characterized mechanisms	284:314	poorly characterized mechanisms	284:314	Vesicle-mediated protein secretion in macrophages is regulated by poorly characterized mechanisms; however, it is now known that inflammatory conditions significantly alter both the quantities and protein composition of secreted vesicles.
36525001	7	53	theme	canonical	1165:1173	arg1	vesiculation					1210:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	Our data demonstrate that this process differs significantly from canonical inflammasome activator ATP-induced vesiculation, and it is dependent on the autocrine IFN signal associated with TLR4 activation.
36525001	8	54	theme	cell	1471:1474	arg1	effectors					1482:1490	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	54	theme	cell	1471:1474	arg1	MLKL					1499:1502	MLKL	1499:1502	MLKL	1499:1502	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	54	theme	cell	1471:1474	arg1	NINJ1					1509:1513	NINJ1	1509:1513	NINJ1	1509:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	54	theme	cell	1471:1474	arg1	GSDMD					1492:1496	GSDMD	1492:1496	GSDMD	1492:1496	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	0	55	theme	Extracellular	82:94	arg1	Secretion					104:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Lipopolysaccharide Primes Human Macrophages for Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion.
36525001	6	56	theme	protein	1080:1086	arg1	secretion					1088:1096	the EV-mediated protein secretion	1064:1096	the EV-mediated protein secretion	1064:1096	Moreover, sensitization of the macrophages through TLR4 by LPS priming prior to LPS transfection dramatically augments the EV-mediated protein secretion.
36525001	6	57	theme	macrophages	976:986	arg1	sensitization					955:967	sensitization	955:967	sensitization of the macrophages through TLR4 by LPS priming prior to LPS transfection	955:1040	Moreover, sensitization of the macrophages through TLR4 by LPS priming prior to LPS transfection dramatically augments the EV-mediated protein secretion.
36525001	7	58	theme	inflammasome	1175:1186	arg1	vesiculation					1210:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	Our data demonstrate that this process differs significantly from canonical inflammasome activator ATP-induced vesiculation, and it is dependent on the autocrine IFN signal associated with TLR4 activation.
36525001	3	59	theme	secretion	578:586	arg1	modulation					544:553	the modulation	540:553	the modulation of EV-mediated protein secretion	540:586	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	0	60	theme	Inflammasome-Induced	61:80	arg1	Secretion					104:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Lipopolysaccharide Primes Human Macrophages for Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion.
36525001	6	61	theme	EV-mediated	1068:1078	arg1	secretion					1088:1096	the EV-mediated protein secretion	1064:1096	the EV-mediated protein secretion	1064:1096	Moreover, sensitization of the macrophages through TLR4 by LPS priming prior to LPS transfection dramatically augments the EV-mediated protein secretion.
36525001	8	62	theme	recipient	1588:1596	arg1	cells					1598:1602	recipient cells	1588:1602	recipient cells	1588:1602	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	7	63	theme	activator	1188:1196	arg1	vesiculation					1210:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	canonical inflammasome activator ATP-induced vesiculation	1165:1221	Our data demonstrate that this process differs significantly from canonical inflammasome activator ATP-induced vesiculation, and it is dependent on the autocrine IFN signal associated with TLR4 activation.
36525001	3	64	theme	EV-mediated	558:568	arg1	secretion					578:586	EV-mediated protein secretion	558:586	EV-mediated protein secretion	558:586	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	8	65	theme	paracrine	1567:1575	arg1	effects					1577:1583	paracrine effects	1567:1583	paracrine effects	1567:1583	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	3	66	theme	LPS	648:650	arg1	transfection					652:663	LPS transfection	648:663	LPS transfection	648:663	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	3	67	theme	protein	570:576	arg1	secretion					578:586	EV-mediated protein secretion	558:586	EV-mediated protein secretion	558:586	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	8	68	theme	components	1434:1443	arg1	secretion					1408:1416	vesicle-mediated secretion	1391:1416	vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1	1391:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	4	69	theme	EV	733:734	arg1	secretion					736:744	robust caspase-4-dependent EV secretion	706:744	robust caspase-4-dependent EV secretion	706:744	We show that human macrophages activate robust caspase-4-dependent EV secretion upon transfection of LPS, and this process is also partially dependent on NLRP3 and caspase-5.
36525001	5	70	with	delivery	870:877	arg1	vesicles					935:942	Escherichia coli-derived outer membrane vesicles	895:942	Escherichia coli-derived outer membrane vesicles	895:942	A similar effect occurs with delivery of the LPS with Escherichia coli-derived outer membrane vesicles.
36525001	8	71	theme	lytic	1465:1469	arg1	effectors					1482:1490	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	71	theme	lytic	1465:1469	arg1	MLKL					1499:1502	MLKL	1499:1502	MLKL	1499:1502	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	71	theme	lytic	1465:1469	arg1	NINJ1					1509:1513	NINJ1	1509:1513	NINJ1	1509:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	71	theme	lytic	1465:1469	arg1	GSDMD					1492:1496	GSDMD	1492:1496	GSDMD	1492:1496	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	3	72	theme	inflammasome	620:631	arg1	activation					633:642	noncanonical caspase-4/5 inflammasome activation	595:642	noncanonical caspase-4/5 inflammasome activation	595:642	In this study, we employed high-throughput quantitative proteomics to characterize the modulation of EV-mediated protein secretion during noncanonical caspase-4/5 inflammasome activation via LPS transfection.
36525001	9	73	theme	anti-inflammatory	1813:1829	arg1	drugs					1831:1835	these anti-inflammatory drugs	1807:1835	these anti-inflammatory drugs	1807:1835	Moreover, using bioinformatics methods, we identify 15-deoxy-Δ12,14-PGJ2 and parthenolide as inhibitors of caspase-4-mediated inflammation and vesicle secretion, indicating new therapeutic potential of these anti-inflammatory drugs.
36525001	0	74	theme	Vesicle	96:102	arg1	Secretion					104:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion	48:112	Lipopolysaccharide Primes Human Macrophages for Noncanonical Inflammasome-Induced Extracellular Vesicle Secretion.
36525001	8	75	dep	effectors	1482:1490	arg1	effectors					1482:1490	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	lytic cell death effectors GSDMD, MLKL, and NINJ1	1465:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	75	dep	effectors	1482:1490	arg1	MLKL					1499:1502	MLKL	1499:1502	MLKL	1499:1502	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	75	dep	effectors	1482:1490	arg1	NINJ1					1509:1513	NINJ1	1509:1513	NINJ1	1509:1513	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	8	75	dep	effectors	1482:1490	arg1	GSDMD					1492:1496	GSDMD	1492:1496	GSDMD	1492:1496	LPS priming preceding the noncanonical inflammasome activation significantly enhances vesicle-mediated secretion of inflammasome components caspase-1, ASC, and lytic cell death effectors GSDMD, MLKL, and NINJ1, suggesting that inflammatory EV transfer may exert paracrine effects in recipient cells.
36525001	1	76	theme	numerous	182:189	arg1	proteins					208:215	numerous immunoregulatory proteins	182:215	numerous immunoregulatory proteins	182:215	Human macrophages secrete extracellular vesicles (EVs) loaded with numerous immunoregulatory proteins.
35952517	10	0	theme	signaling	2350:2358	arg1	pathway					2360:2366	Wnt/β-catenin signaling pathway	2336:2366	Wnt/β-catenin signaling pathway	2336:2366	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	1	dep	proteins	2147:2154	arg1	concentrated					2164:2175	concentrated	2164:2175	concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways	2164:2248	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	2	2	theme	new	360:362	arg1	direction					364:372	a new direction	358:372	a new direction	358:372	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	1	3	theme	system	261:266	arg1	cancer					163:168	BACKGROUND Colorectal cancer	141:168	BACKGROUND Colorectal cancer (CRC)	141:174	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	3	theme	system	261:266	arg1	diseases					233:240	the most common diseases	217:240	the most common diseases of human digestive system	217:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	3	theme	system	261:266	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	6	4	theme	Firmicutes	1178:1187	arg1	ratio					1169:1173	the upregulated ratio	1153:1173	the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis	1153:1257	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	4	5	theme	SK	850:851	arg1	function					838:845	the function	834:845	the function of SK and acetyl-SK on the gut microbes and protein expression, respectively	834:922	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	10	6	theme	proteins	2147:2154	arg1	upregulation					2077:2088	the upregulation	2073:2088	the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways)	2073:2249	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	6	theme	proteins	2147:2154	arg1	downregulation					2260:2273	the downregulation	2256:2273	the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway	2256:2366	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	6	theme	proteins	2147:2154	arg1	increase					1999:2006	the increase	1995:2006	the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α)	1995:2070	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	4	7	theme	acetyl-SK	857:865	arg1	function					838:845	the function	834:845	the function of SK and acetyl-SK on the gut microbes and protein expression, respectively	834:922	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	6	8	theme	gut	1234:1236	arg1	dysbiosis					1249:1257	gut microbiota dysbiosis	1234:1257	gut microbiota dysbiosis	1234:1257	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	2	9	theme	microenvironment	313:328	arg1	influence					283:291	Nowadays, the influence	269:291	influence	283:291	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	3	10	theme	therapeutic	742:752	arg1	effects					754:760	their therapeutic effects	736:760	their therapeutic effects	736:760	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	4	11	from	function	838:845	arg1	expression					899:908	protein expression	891:908	protein expression	891:908	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	4	11	from	function	838:845	arg1	microbes					878:885	gut microbes	874:885	gut microbes	874:885	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	4	12	theme	protein	891:897	arg1	expression					899:908	protein expression	891:908	protein expression	891:908	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	1	13	theme	Colorectal	152:161	arg1	cancer					163:168	BACKGROUND Colorectal cancer	141:168	BACKGROUND Colorectal cancer (CRC)	141:174	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	13	theme	Colorectal	152:161	arg1	diseases					233:240	the most common diseases	217:240	the most common diseases of human digestive system	217:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	13	theme	Colorectal	152:161	arg1	CRC					171:173	CRC	171:173	CRC	171:173	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	13	theme	Colorectal	152:161	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	6	14	theme	F/B	1206:1208	arg1	Bacteroidota					1192:1203	Bacteroidota	1192:1203	Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis	1192:1257	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	6	14	theme	F/B	1206:1208	arg1	ratio					1210:1214	F/B ratio	1206:1214	F/B ratio	1206:1214	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	9	15	from	dose-dependent	1795:1808	arg1	study					1754:1758	our current study	1742:1758	our current study	1742:1758	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	8	16	from	increase	1513:1520	arg1	abundance					1529:1537	the abundance	1525:1537	the abundance	1525:1537	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	9	17	theme	20 mg/kg	1815:1822	arg1	SK					1824:1825	20 mg/kg SK	1815:1825	20 mg/kg SK	1815:1825	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	8	18	theme	valens	1555:1560	arg1	abundance					1529:1537	the abundance	1525:1537	the abundance	1525:1537	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	10	19	theme	IL-17	2225:2229	arg1	pathways					2241:2248	IL-17 signaling pathways	2225:2248	IL-17 signaling pathways	2225:2248	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	20	theme	pyruvate	2093:2100	arg1	PKM2					2126:2129	PKM2	2126:2129	PKM2	2126:2129	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	20	theme	pyruvate	2093:2100	arg1	M2					2122:2123	pyruvate kinase isozyme type M2	2093:2123	pyruvate kinase isozyme type M2 (PKM2)	2093:2130	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	1	21	theme	inflammatory	180:191	arg1	cancer					163:168	BACKGROUND Colorectal cancer	141:168	BACKGROUND Colorectal cancer (CRC)	141:174	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	21	theme	inflammatory	180:191	arg1	IBD					208:210	IBD	208:210	IBD	208:210	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	21	theme	inflammatory	180:191	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	21	theme	inflammatory	180:191	arg1	diseases					233:240	the most common diseases	217:240	the most common diseases of human digestive system	217:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	8	22	theme	g__norank_f__norank_o__Clostridia_UCG-014	1566:1606	arg1	abundance					1529:1537	the abundance	1525:1537	the abundance	1525:1537	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	11	23	theme	preventive	2447:2456	arg1	SK					2401:2402	SK	2401:2402	SK	2401:2402	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	11	23	theme	preventive	2447:2456	arg1	acetyl-SK					2408:2416	acetyl-SK	2408:2416	acetyl-SK	2408:2416	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	11	23	theme	preventive	2447:2456	arg1	agents					2458:2463	potential preventive agents	2437:2463	potential preventive agents for CAC	2437:2471	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	4	24	theme	fresh	773:777	arg1	tissues					795:801	fresh feces and colon tissues	773:801	fresh feces and colon tissues	773:801	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	9	25	dep	dose-dependent	1795:1808	arg1	exhibited					1827:1835	exhibited	1827:1835	exhibited the most effective functions, even better than the positive drug mesalazine	1827:1911	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	2	26	dep	potential	485:493	arg1	anti-cancer					495:505	anti-cancer	495:505	anti-cancer	495:505	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	2	27	theme	relative	398:405	arg1	mechanism					417:425	relative molecular mechanism	398:425	relative molecular mechanism	398:425	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	10	28	dep	cytokines	2028:2036	arg1	including					2039:2047	including	2039:2047	including IL-1β, IL-6 and TNF-α	2039:2069	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	0	29	theme	Natural	0:6	arg1	shikonin					8:15	Natural shikonin	0:15	Natural shikonin	0:15	Natural shikonin and acetyl-shikonin improve intestinal microbial and protein composition to alleviate colitis-associated colorectal cancer.
35952517	3	30	theme	colitis-associated	657:674	arg1	cancer					687:692	colitis-associated colorectal cancer	657:692	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage	600:719	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	30	theme	colitis-associated	657:674	arg1	CAC					695:697	CAC	695:697	CAC	695:697	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	2	31	theme	mechanism	417:425	arg1	exploration					383:393	the exploration	379:393	the exploration of relative molecular mechanism	379:425	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	10	32	dep	concentrated	2164:2175	arg1	mainly					2157:2162	mainly	2157:2162	mainly	2157:2162	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	9	33	theme	positive	1888:1895	arg1	mesalazine					1902:1911	the positive drug mesalazine	1884:1911	the positive drug mesalazine	1884:1911	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	8	34	theme	colonic	1658:1664	arg1	inflammation					1666:1677	colonic inflammation	1658:1677	colonic inflammation	1658:1677	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	0	35	theme	protein	70:76	arg1	composition					78:88	intestinal microbial and protein composition	45:88	composition	78:88	Natural shikonin and acetyl-shikonin improve intestinal microbial and protein composition to alleviate colitis-associated colorectal cancer.
35952517	5	36	theme	mouse	1038:1042	arg1	model					1044:1048	CAC mouse model	1034:1048	CAC mouse model	1034:1048	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	10	37	theme	M2	2122:2123	arg1	upregulation					2077:2088	the upregulation	2073:2088	the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways)	2073:2249	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	37	theme	M2	2122:2123	arg1	downregulation					2260:2273	the downregulation	2256:2273	the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway	2256:2366	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	37	theme	M2	2122:2123	arg1	increase					1999:2006	the increase	1995:2006	the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α)	1995:2070	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	1	38	theme	common	226:231	arg1	cancer					163:168	BACKGROUND Colorectal cancer	141:168	BACKGROUND Colorectal cancer (CRC)	141:174	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	38	theme	common	226:231	arg1	diseases					233:240	the most common diseases	217:240	the most common diseases of human digestive system	217:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	38	theme	common	226:231	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	10	39	theme	isozyme	2109:2115	arg1	PKM2					2126:2129	PKM2	2126:2129	PKM2	2126:2129	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	39	theme	isozyme	2109:2115	arg1	M2					2122:2123	pyruvate kinase isozyme type M2	2093:2123	pyruvate kinase isozyme type M2 (PKM2)	2093:2130	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	3	40	theme	cancer	687:692	arg1	model					705:709	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model	600:709	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage	600:719	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	2	41	theme	novel	479:483	arg1	molecules					507:515	novel potential anti-cancer molecules	479:515	novel potential anti-cancer molecules	479:515	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	7	42	dep	bacteria	1307:1314	arg1	Lachnospiraceae_NK4A136_Group					1360:1388	Lachnospiraceae_NK4A136_Group	1360:1388	Lachnospiraceae_NK4A136_Group	1360:1388	SK and its derivative increased the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group, and reduced those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002.
35952517	7	42	dep	bacteria	1307:1314	arg1	Lactobacillus					1345:1357	Lactobacillus	1345:1357	Lactobacillus	1345:1357	SK and its derivative increased the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group, and reduced those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002.
35952517	7	42	dep	bacteria	1307:1314	arg1	g__norank_f__Muribaculaceae					1316:1342	g__norank_f__Muribaculaceae	1316:1342	g__norank_f__Muribaculaceae	1316:1342	SK and its derivative increased the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group, and reduced those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002.
35952517	1	43	theme	digestive	251:259	arg1	system					261:266	human digestive system	245:266	human digestive system	245:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	10	44	theme	Wnt/β-catenin	2336:2348	arg1	pathway					2360:2366	Wnt/β-catenin signaling pathway	2336:2366	Wnt/β-catenin signaling pathway	2336:2366	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	6	45	theme	species	1081:1087	arg1	They					1051:1054	They	1051:1054	They	1051:1054	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	6	45	theme	species	1081:1087	arg1	richness					1089:1096	improved species richness	1072:1096	improved species richness	1072:1096	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	3	46	theme	mice	700:703	arg1	model					705:709	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model	600:709	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage	600:719	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	4	47	theme	gut	874:876	arg1	microbes					878:885	gut microbes	874:885	gut microbes	874:885	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	1	48	theme	bowel	193:197	arg1	cancer					163:168	BACKGROUND Colorectal cancer	141:168	BACKGROUND Colorectal cancer (CRC)	141:174	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	48	theme	bowel	193:197	arg1	IBD					208:210	IBD	208:210	IBD	208:210	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	48	theme	bowel	193:197	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	48	theme	bowel	193:197	arg1	diseases					233:240	the most common diseases	217:240	the most common diseases of human digestive system	217:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	9	49	theme	current	1746:1752	arg1	study					1754:1758	our current study	1742:1758	our current study	1742:1758	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	6	50	theme	upregulated	1157:1167	arg1	ratio					1169:1173	the upregulated ratio	1153:1173	the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis	1153:1257	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	10	51	theme	other	2141:2145	arg1	proteins					2147:2154	some other proteins	2136:2154	some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways)	2136:2249	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	52	from	mis-regulation	2196:2209	arg1	cancer					2214:2219	cancer	2214:2219	cancer	2214:2219	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	52	from	mis-regulation	2196:2209	arg1	pathways					2241:2248	IL-17 signaling pathways	2225:2248	IL-17 signaling pathways	2225:2248	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	6	53	theme	microbiota	1238:1247	arg1	dysbiosis					1249:1257	gut microbiota dysbiosis	1234:1257	gut microbiota dysbiosis	1234:1257	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	9	54	from	study	1754:1758	arg1	dose-dependent					1795:1808	dose-dependent	1795:1808	dose-dependent	1795:1808	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	10	55	theme	transcriptional	2180:2194	arg1	mis-regulation					2196:2209	transcriptional mis-regulation	2180:2209	transcriptional mis-regulation in cancer and IL-17 signaling pathways	2180:2248	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	56	theme	cytokines	2028:2036	arg1	upregulation					2077:2088	the upregulation	2073:2088	the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways)	2073:2249	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	56	theme	cytokines	2028:2036	arg1	downregulation					2260:2273	the downregulation	2256:2273	the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway	2256:2366	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	56	theme	cytokines	2028:2036	arg1	increase					1999:2006	the increase	1995:2006	the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α)	1995:2070	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	2	57	theme	inflammatory	300:311	arg1	microenvironment					313:328	the inflammatory microenvironment	296:328	the inflammatory microenvironment	296:328	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	8	58	theme	significant	1501:1511	arg1	increase					1513:1520	significant increase	1501:1520	significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer	1501:1687	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	6	59	theme	flora	1136:1140	arg1	They					1051:1054	They	1051:1054	They	1051:1054	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	6	59	theme	flora	1136:1140	arg1	SK					1068:1069	SK	1068:1069	SK	1068:1069	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	6	59	theme	flora	1136:1140	arg1	evenness					1099:1106	evenness	1099:1106	evenness	1099:1106	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	6	59	theme	flora	1136:1140	arg1	richness					1089:1096	improved species richness	1072:1096	improved species richness	1072:1096	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	6	59	theme	flora	1136:1140	arg1	diversity					1112:1120	diversity	1112:1120	diversity of intestinal flora	1112:1140	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	9	60	theme	SK	1775:1776	arg1	action					1765:1770	the action	1761:1770	the action of SK and acetyl-SK	1761:1790	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	11	61	theme	gut	2497:2499	arg1	microbes					2501:2508	gut microbes	2497:2508	gut microbes	2497:2508	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	11	62	theme	Wnt/β-catenin	2567:2579	arg1	pathway					2591:2597	Wnt/β-catenin signaling pathway	2567:2597	Wnt/β-catenin signaling pathway	2567:2597	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	1	63	theme	BACKGROUND	141:150	arg1	cancer					163:168	BACKGROUND Colorectal cancer	141:168	BACKGROUND Colorectal cancer (CRC)	141:174	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	63	theme	BACKGROUND	141:150	arg1	diseases					233:240	the most common diseases	217:240	the most common diseases of human digestive system	217:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	63	theme	BACKGROUND	141:150	arg1	CRC					171:173	CRC	171:173	CRC	171:173	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	1	63	theme	BACKGROUND	141:150	arg1	disease					199:205	inflammatory bowel disease	180:205	inflammatory bowel disease (IBD)	180:211	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35952517	9	64	theme	acetyl-SK	1782:1790	arg1	action					1765:1770	the action	1761:1770	the action of SK and acetyl-SK	1761:1790	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	7	65	theme	harmful	1409:1415	arg1	Coriobacteriaceae					1450:1466	Coriobacteriaceae	1450:1466	Coriobacteriaceae	1450:1466	SK and its derivative increased the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group, and reduced those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002.
35952517	7	65	theme	harmful	1409:1415	arg1	Ileibacterium					1432:1444	Ileibacterium	1432:1444	Ileibacterium	1432:1444	SK and its derivative increased the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group, and reduced those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002.
35952517	7	65	theme	harmful	1409:1415	arg1	ones					1417:1420	those harmful ones	1403:1420	those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002	1403:1474	SK and its derivative increased the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group, and reduced those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002.
35952517	2	66	theme	Nowadays	269:276	arg1	influence					283:291	Nowadays, the influence	269:291	influence	283:291	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	5	67	dep	RESULTS	925:931	arg1	acetyl-SK					945:953	acetyl-SK	945:953	acetyl-SK	945:953	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	5	67	dep	RESULTS	925:931	arg1	SK					938:939	SK	938:939	SK	938:939	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	5	67	dep	RESULTS	925:931	arg1	RESULTS					925:931	RESULTS Both SK and acetyl-SK	925:953	RESULTS Both SK and acetyl-SK	925:953	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	2	68	from	influence	283:291	arg1	tumorigenesis					333:345	tumorigenesis	333:345	tumorigenesis	333:345	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	10	69	theme	signaling	2231:2239	arg1	pathways					2241:2248	IL-17 signaling pathways	2225:2248	IL-17 signaling pathways	2225:2248	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	0	70	theme	colitis-associated	103:120	arg1	cancer					133:138	colitis-associated colorectal cancer	103:138	colitis-associated colorectal cancer	103:138	Natural shikonin and acetyl-shikonin improve intestinal microbial and protein composition to alleviate colitis-associated colorectal cancer.
35952517	10	71	theme	pro-inflammatory	2011:2026	arg1	cytokines					2028:2036	pro-inflammatory cytokines	2011:2036	pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α)	2011:2070	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	2	72	theme	molecules	507:515	arg1	identification					461:474	identification	461:474	identification	461:474	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	2	72	theme	molecules	507:515	arg1	discovery					447:455	discovery	447:455	discovery	447:455	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	11	73	theme	potential	2437:2445	arg1	SK					2401:2402	SK	2401:2402	SK	2401:2402	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	11	73	theme	potential	2437:2445	arg1	acetyl-SK					2408:2416	acetyl-SK	2408:2416	acetyl-SK	2408:2416	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	11	73	theme	potential	2437:2445	arg1	agents					2458:2463	potential preventive agents	2437:2463	potential preventive agents for CAC	2437:2471	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	0	74	theme	colorectal	122:131	arg1	cancer					133:138	colitis-associated colorectal cancer	103:138	colitis-associated colorectal cancer	103:138	Natural shikonin and acetyl-shikonin improve intestinal microbial and protein composition to alleviate colitis-associated colorectal cancer.
35952517	5	75	theme	AOM/DSS-induced	965:979	arg1	CAC					981:983	AOM/DSS-induced CAC	965:983	AOM/DSS-induced CAC	965:983	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	11	76	theme	signaling	2581:2589	arg1	pathway					2591:2597	Wnt/β-catenin signaling pathway	2567:2597	Wnt/β-catenin signaling pathway	2567:2597	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	2	77	dep	discovery	447:455	arg1	the					443:445	the	443:445	the	443:445	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	3	78	dep	METHODS	518:524	arg1	METHODS					518:524	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK)	518:578	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK)	518:578	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	78	dep	METHODS	518:524	arg1	acetyl-SK					569:577	acetyl-SK	569:577	acetyl-SK	569:577	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	78	dep	METHODS	518:524	arg1	SK					544:545	SK	544:545	SK	544:545	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	78	dep	METHODS	518:524	arg1	acetyl-shikonin					552:566	acetyl-shikonin	552:566	acetyl-shikonin (acetyl-SK)	552:578	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	78	dep	METHODS	518:524	arg1	shikonin					534:541	Natural shikonin	526:541	Natural shikonin (SK)	526:546	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	6	79	theme	improved	1072:1079	arg1	They					1051:1054	They	1051:1054	They	1051:1054	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	6	79	theme	improved	1072:1079	arg1	richness					1089:1096	improved species richness	1072:1096	improved species richness	1072:1096	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	9	80	theme	effective	1846:1854	arg1	functions					1856:1864	the most effective functions	1837:1864	the most effective functions	1837:1864	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	10	81	theme	Μgt2b34-Aldh1a1-Aldh1a7	2300:2322	arg1	upregulation					2077:2088	the upregulation	2073:2088	the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways)	2073:2249	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	81	theme	Μgt2b34-Aldh1a1-Aldh1a7	2300:2322	arg1	downregulation					2260:2273	the downregulation	2256:2273	the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway	2256:2366	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	81	theme	Μgt2b34-Aldh1a1-Aldh1a7	2300:2322	arg1	increase					1999:2006	the increase	1995:2006	the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α)	1995:2070	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	4	82	theme	feces	779:783	arg1	tissues					795:801	fresh feces and colon tissues	773:801	fresh feces and colon tissues	773:801	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	10	83	theme	differential	1924:1935	arg1	proteomics					1937:1946	differential proteomics	1924:1946	differential proteomics	1924:1946	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	84	theme	Aldh1b1-Acc3-Maoa	2278:2294	arg1	upregulation					2077:2088	the upregulation	2073:2088	the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways)	2073:2249	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	84	theme	Aldh1b1-Acc3-Maoa	2278:2294	arg1	downregulation					2260:2273	the downregulation	2256:2273	the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway	2256:2366	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	84	theme	Aldh1b1-Acc3-Maoa	2278:2294	arg1	increase					1999:2006	the increase	1995:2006	the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α)	1995:2070	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	7	85	theme	beneficial	1296:1305	arg1	bacteria					1307:1314	the beneficial bacteria	1292:1314	the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group	1292:1388	SK and its derivative increased the beneficial bacteria g__norank_f__Muribaculaceae, Lactobacillus, Lachnospiraceae_NK4A136_Group, and reduced those harmful ones including Ileibacterium and Coriobacteriaceae UCG-002.
35952517	9	86	theme	better	1872:1877	arg1	functions					1856:1864	the most effective functions	1837:1864	the most effective functions	1837:1864	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	3	87	theme	-induced	648:655	arg1	model					705:709	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model	600:709	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage	600:719	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	4	88	dep	microbes	878:885	arg1	the					870:872	the	870:872	the	870:872	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	2	89	theme	potential	485:493	arg1	molecules					507:515	novel potential anti-cancer molecules	479:515	novel potential anti-cancer molecules	479:515	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	8	90	theme	inflammation	1666:1677	arg1	studies					1647:1653	studies	1647:1653	studies of colonic inflammation or cancer	1647:1687	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	2	91	theme	molecular	407:415	arg1	mechanism					417:425	relative molecular mechanism	398:425	relative molecular mechanism	398:425	Nowadays, the influence of the inflammatory microenvironment on tumorigenesis has become a new direction, and the exploration of relative molecular mechanism will facilitate the discovery and identification of novel potential anti-cancer molecules.
35952517	5	92	theme	CAC	1034:1036	arg1	model					1044:1048	CAC mouse model	1034:1048	CAC mouse model	1034:1048	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	6	93	theme	intestinal	1125:1134	arg1	flora					1136:1140	intestinal flora	1125:1140	intestinal flora	1125:1140	They, especially SK, improved species richness, evenness and diversity of intestinal flora, recovered the upregulated ratio of Firmicutes to Bacteroidota (F/B ratio) which symbolizes gut microbiota dysbiosis.
35952517	9	94	theme	drug	1897:1900	arg1	mesalazine					1902:1911	the positive drug mesalazine	1884:1911	the positive drug mesalazine	1884:1911	In our current study, the action of SK and acetyl-SK is dose-dependent, and 20 mg/kg SK exhibited the most effective functions, even better than the positive drug mesalazine.
35952517	8	95	theme	cancer	1682:1687	arg1	studies					1647:1653	studies	1647:1653	studies of colonic inflammation or cancer	1647:1687	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	10	96	theme	ELISA	1952:1956	arg1	results					1958:1964	ELISA results	1952:1964	ELISA results	1952:1964	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	3	97	theme	Natural	526:532	arg1	METHODS					518:524	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK)	518:578	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK)	518:578	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	97	theme	Natural	526:532	arg1	SK					544:545	SK	544:545	SK	544:545	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	97	theme	Natural	526:532	arg1	shikonin					534:541	Natural shikonin	526:541	Natural shikonin (SK)	526:546	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	8	98	theme	SK	1735:1736	arg1	20 mg/kg					1723:1730	20 mg/kg	1723:1730	20 mg/kg of SK	1723:1736	Notably, AOM/DSS caused significant increase in the abundance of Ileibaterium valens and g__norank_f__norank_o__Clostridia_UCG-014, which were not previously reported in studies of colonic inflammation or cancer, and the disorder was reversed by 20 mg/kg of SK.
35952517	5	99	theme	flora	1015:1019	arg1	structure					1021:1029	the intestinal flora structure	1000:1029	the intestinal flora structure	1000:1029	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	10	100	theme	type	2117:2120	arg1	PKM2					2126:2129	PKM2	2126:2129	PKM2	2126:2129	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	100	theme	type	2117:2120	arg1	M2					2122:2123	pyruvate kinase isozyme type M2	2093:2123	pyruvate kinase isozyme type M2 (PKM2)	2093:2130	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	3	101	theme	colorectal	676:685	arg1	cancer					687:692	colitis-associated colorectal cancer	657:692	azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage	600:719	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	3	101	theme	colorectal	676:685	arg1	CAC					695:697	CAC	695:697	CAC	695:697	METHODS Natural shikonin (SK) and acetyl-shikonin (acetyl-SK) was administered to azoxymethane (AOM)/dextran sodium sulphate (DSS)-induced colitis-associated colorectal cancer (CAC) mice model by gavage to investigate their therapeutic effects.
35952517	4	102	theme	colon	789:793	arg1	tissues					795:801	fresh feces and colon tissues	773:801	fresh feces and colon tissues	773:801	Moreover, fresh feces and colon tissues were collected for determining the function of SK and acetyl-SK on the gut microbes and protein expression, respectively.
35952517	5	103	theme	intestinal	1004:1013	arg1	structure					1021:1029	the intestinal flora structure	1000:1029	the intestinal flora structure	1000:1029	RESULTS Both SK and acetyl-SK decreased AOM/DSS-induced CAC, and regulated the intestinal flora structure in CAC mouse model.
35952517	10	104	theme	kinase	2102:2107	arg1	PKM2					2126:2129	PKM2	2126:2129	PKM2	2126:2129	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	10	104	theme	kinase	2102:2107	arg1	M2					2122:2123	pyruvate kinase isozyme type M2	2093:2123	pyruvate kinase isozyme type M2 (PKM2)	2093:2130	Moreover, differential proteomics and ELISA results showed that SK could recover the increase of pro-inflammatory cytokines (including IL-1β, IL-6 and TNF-α), the upregulation of pyruvate kinase isozyme type M2 (PKM2) and some other proteins (mainly concentrated in transcriptional mis-regulation in cancer and IL-17 signaling pathways), and the downregulation of Aldh1b1-Acc3-Maoa and Μgt2b34-Aldh1a1-Aldh1a7 involved in Wnt/β-catenin signaling pathway.
35952517	11	105	dep	CONCLUSION	2369:2378	arg1	identified					2390:2399	identified	2390:2399	identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway	2390:2597	CONCLUSION Our study identified SK and acetyl-SK, especially SK, as potential preventive agents for CAC through regulating both gut microbes and pathways involved in inflammation and cancer such as Wnt/β-catenin signaling pathway.
35952517	1	106	theme	human	245:249	arg1	system					261:266	human digestive system	245:266	human digestive system	245:266	BACKGROUND Colorectal cancer (CRC) and inflammatory bowel disease (IBD) are the most common diseases of human digestive system.
35062972	11	0	theme	promising	2104:2112	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	0	theme	promising	2104:2112	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	6	1	theme	amputation	1068:1077	arg1	model					1079:1083	rat tail amputation model	1059:1083	rat tail amputation model	1059:1083	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	6	2	theme	OBNC-DFO	1167:1174	arg1	effects					1147:1153	superior pro-coagulation effects	1122:1153	superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL)	1122:1227	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	11	3	theme	hemostatic	1997:2006	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	3	theme	hemostatic	1997:2006	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	4	theme	repair	2146:2151	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	4	theme	repair	2146:2151	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	6	5	theme	OBNC	1158:1161	arg1	effects					1147:1153	superior pro-coagulation effects	1122:1153	superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL)	1122:1227	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	10	6	theme	regeneration	1881:1892	arg1	achievement					1840:1850	the achievement	1836:1850	the achievement of structural and functional regeneration of the skin	1836:1904	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	3	7	theme	clot	540:543	arg1	formation					545:553	clot formation	540:553	clot formation	540:553	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	7	8	theme	pathways	1393:1400	arg1	loss					1417:1420	less blood loss	1406:1420	less blood loss	1406:1420	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	7	8	theme	pathways	1393:1400	arg1	activation					1356:1365	shorter whole blood clotting time, more robust activation	1309:1365	shorter whole blood clotting time, more robust activation of endogenous coagulation pathways	1309:1400	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	5	9	theme	hemostatic	873:882	arg1	sponge					884:889	This new hemostatic sponge	864:889	This new hemostatic sponge	864:889	This new hemostatic sponge can absorb water at a rate of approximate 1.70 g/s, rapidly enhancing clot formation in the early stage of hemostasis.
35062972	6	10	theme	superior	1122:1129	arg1	effects					1147:1153	superior pro-coagulation effects	1122:1153	superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL)	1122:1227	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	3	11	theme	coagulation	569:579	arg1	reaction					581:588	coagulation reaction	569:588	coagulation reaction	569:588	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	11	12	theme	coagulation	2040:2050	arg1	pathways					2052:2059	coagulation pathways	2040:2059	coagulation pathways	2040:2059	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	8	13	theme	In	1423:1424	arg1	assays					1441:1446	In vitro cellular assays	1423:1446	In vitro cellular assays	1423:1446	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	2	14	dep	RESULTS	252:258	arg1	constructed					278:288	constructed	278:288	constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC)	278:441	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	3	15	theme	due	616:618	arg1	absorption					605:614	rapid blood absorption	593:614	rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET)	593:690	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	8	16	theme	cellular	1432:1439	arg1	assays					1441:1446	In vitro cellular assays	1423:1446	In vitro cellular assays	1423:1446	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	3	17	theme	blood	599:603	arg1	absorption					605:614	rapid blood absorption	593:614	rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET)	593:690	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	1	18	theme	wound	134:138	arg1	healing					140:146	wound healing	134:146	wound healing	134:146	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	11	19	theme	skin	2073:2076	arg1	regeneration					2078:2089	skin regeneration	2073:2089	skin regeneration	2073:2089	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	10	20	theme	hair	1789:1792	arg1	follicles					1794:1802	hair follicles	1789:1802	hair follicles	1789:1802	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	10	20	theme	hair	1789:1792	arg1	appendages					1771:1780	skin appendages	1766:1780	skin appendages (e.g., hair follicles)	1766:1803	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	6	21	theme	collagen	1195:1202	arg1	sponges					1215:1221	clinically used collagen hemostatic sponges	1179:1221	clinically used collagen hemostatic sponges (COL)	1179:1227	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	6	21	theme	collagen	1195:1202	arg1	COL					1224:1226	COL	1224:1226	COL	1224:1226	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	1	22	dep	BACKGROUND	70:79	arg1	repair					96:101	repair	96:101	repair	96:101	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	1	22	dep	BACKGROUND	70:79	arg1	Hemostasis					81:90	Hemostasis	81:90	Hemostasis	81:90	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	3	23	theme	total	632:636	arg1	area					643:646	the high total pore area	623:646	the high total pore area (approximately 42.429 m2/g measured by BET)	623:690	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	6	24	theme	In	1010:1011	arg1	experiments					1043:1053	In vitro and in vivo coagulation experiments	1010:1053	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model)	1010:1107	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	7	25	theme	blood	1323:1327	arg1	time					1338:1341	whole blood clotting time	1317:1341	whole blood clotting time	1317:1341	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	7	26	theme	cells	1284:1288	arg1	activation					1260:1269	activation	1260:1269	activation of red blood cells and platelets	1260:1302	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	7	26	theme	cells	1284:1288	arg1	aggregation					1244:1254	aggregation	1244:1254	aggregation	1244:1254	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	1	27	theme	early	153:157	arg1	hemostasis					159:168	early hemostasis	153:168	early hemostasis	153:168	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	4	28	theme	further	697:703	arg1	release					705:711	The further release	693:711	The further release of DFO	693:718	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	11	29	theme	oxidized	1964:1971	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	29	theme	oxidized	1964:1971	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	6	30	dep	in	1023:1024	arg1	vivo					1026:1029	vivo	1026:1029	vivo	1026:1029	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	5	31	theme	clot	961:964	arg1	formation					966:974	clot formation	961:974	clot formation	961:974	This new hemostatic sponge can absorb water at a rate of approximate 1.70 g/s, rapidly enhancing clot formation in the early stage of hemostasis.
35062972	3	32	theme	drug	462:465	arg1	DFO					484:486	DFO	484:486	DFO	484:486	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	3	32	theme	drug	462:465	arg1	desferrioxamine					467:481	Then angiogenetic drug desferrioxamine	444:481	Then angiogenetic drug desferrioxamine (DFO)	444:487	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	7	33	theme	whole	1317:1321	arg1	time					1338:1341	whole blood clotting time	1317:1341	whole blood clotting time	1317:1341	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	6	34	dep	In	1010:1011	arg1	vitro					1013:1017	vitro	1013:1017	vitro	1013:1017	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	2	35	theme	oxidized	403:410	arg1	OBNC					437:440	OBNC	437:440	OBNC	437:440	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	2	35	theme	oxidized	403:410	arg1	nanocellulose					422:434	oxidized bacterial nanocellulose	403:434	oxidized bacterial nanocellulose (OBNC)	403:441	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	0	36	theme	Bacteria-engineered	0:18	arg1	porous					20:25	Bacteria-engineered porous	0:25	Bacteria-engineered porous	0:25	Bacteria-engineered porous sponge for hemostasis and vascularization.
35062972	5	37	theme	1.70 g/s	933:940	arg1	rate					913:916	a rate	911:916	a rate of approximate 1.70 g/s	911:940	This new hemostatic sponge can absorb water at a rate of approximate 1.70 g/s, rapidly enhancing clot formation in the early stage of hemostasis.
35062972	8	38	theme	umbilical	1529:1537	arg1	HUVECs					1563:1568	HUVECs	1563:1568	HUVECs	1563:1568	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	8	38	theme	umbilical	1529:1537	arg1	cells					1556:1560	human umbilical vein endothelial cells	1523:1560	human umbilical vein endothelial cells (HUVECs)	1523:1569	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	4	39	theme	HIF-1α	748:753	arg1	reconstruction					763:776	the reconstruction	759:776	the reconstruction of blood flow	759:790	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	4	39	theme	HIF-1α	748:753	arg1	secretion					735:743	the secretion	731:743	the secretion of HIF-1α	731:753	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	8	40	theme	endothelial	1544:1554	arg1	HUVECs					1563:1568	HUVECs	1563:1568	HUVECs	1563:1568	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	8	40	theme	endothelial	1544:1554	arg1	cells					1556:1560	human umbilical vein endothelial cells	1523:1560	human umbilical vein endothelial cells (HUVECs)	1523:1569	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	6	41	theme	in	1023:1024	arg1	experiments					1043:1053	In vitro and in vivo coagulation experiments	1010:1053	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model)	1010:1107	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	4	42	theme	rapid	808:812	arg1	hemostasis					814:823	hemostasis	814:823	hemostasis	814:823	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	7	43	theme	blood	1278:1282	arg1	cells					1284:1288	red blood cells	1274:1288	red blood cells	1274:1288	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	11	44	theme	pro-regenerative	2129:2144	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	44	theme	pro-regenerative	2129:2144	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	4	45	theme	flow	787:790	arg1	reconstruction					763:776	the reconstruction	759:776	the reconstruction of blood flow	759:790	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	4	45	theme	flow	787:790	arg1	secretion					735:743	the secretion	731:743	the secretion of HIF-1α	731:753	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	2	46	theme	Komagataeibacter	338:353	arg1	xylinus					355:361	Komagataeibacter xylinus	338:361	Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC)	338:441	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	1	47	from	processes	121:129	arg1	healing					140:146	wound healing	134:146	wound healing	134:146	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	1	48	theme	integrated	233:242	arg1	manner					244:249	an integrated manner	230:249	an integrated manner	230:249	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	10	49	theme	injury	1709:1714	arg1	model					1716:1720	the rat skin injury model	1696:1720	the rat skin injury model	1696:1720	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	5	50	theme	early	983:987	arg1	stage					989:993	the early stage	979:993	the early stage of hemostasis	979:1007	This new hemostatic sponge can absorb water at a rate of approximate 1.70 g/s, rapidly enhancing clot formation in the early stage of hemostasis.
35062972	6	51	theme	liver	1089:1093	arg1	model					1102:1106	liver trauma model	1089:1106	liver trauma model	1089:1106	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	10	52	theme	skin	1704:1707	arg1	model					1716:1720	the rat skin injury model	1696:1720	the rat skin injury model	1696:1720	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	10	53	theme	structural	1855:1864	arg1	regeneration					1881:1892	structural and functional regeneration	1855:1892	structural and functional regeneration of the skin	1855:1904	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	2	54	theme	TEMPO	377:381	arg1	oxidation					383:391	TEMPO oxidation	377:391	TEMPO oxidation	377:391	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	11	55	theme	hemostatic	2114:2123	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	55	theme	hemostatic	2114:2123	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	6	56	theme	tail	1063:1066	arg1	model					1079:1083	rat tail amputation model	1059:1083	rat tail amputation model	1059:1083	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	10	57	dep	follicles	1794:1802	arg1	e.g.					1783:1786	e.g.	1783:1786	e.g.	1783:1786	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	6	58	from	experiments	1043:1053	arg1	model					1079:1083	rat tail amputation model	1059:1083	rat tail amputation model	1059:1083	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	6	58	from	experiments	1043:1053	arg1	model					1102:1106	liver trauma model	1089:1106	liver trauma model	1089:1106	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	2	59	theme	hemostatic	292:301	arg1	OBNC-DFO					310:317	a hemostatic sponge OBNC-DFO	290:317	a hemostatic sponge OBNC-DFO	290:317	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	9	60	theme	HIF-1α	1630:1635	arg1	secretion					1617:1625	the secretion	1613:1625	the secretion of HIF-1α	1613:1635	In addition, the release of DFO enhanced the secretion of HIF-1α, further strengthening vascularization in damaged skin.
35062972	11	61	theme	bacterial	1973:1981	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	61	theme	bacterial	1973:1981	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	62	dep	CONCLUSION	1907:1916	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	62	dep	CONCLUSION	1907:1916	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	1	63	theme	following	174:182	arg1	vascularization					184:198	following vascularization	174:198	following vascularization	174:198	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	7	64	theme	blood	1411:1415	arg1	loss					1417:1420	less blood loss	1406:1420	less blood loss	1406:1420	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	10	65	theme	functional	1870:1879	arg1	regeneration					1881:1892	structural and functional regeneration	1855:1892	structural and functional regeneration of the skin	1855:1904	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	5	66	theme	new	869:871	arg1	sponge					884:889	This new hemostatic sponge	864:889	This new hemostatic sponge	864:889	This new hemostatic sponge can absorb water at a rate of approximate 1.70 g/s, rapidly enhancing clot formation in the early stage of hemostasis.
35062972	4	67	theme	damaged	848:854	arg1	tissue					856:861	damaged tissue	848:861	damaged tissue	848:861	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	6	68	theme	pro-coagulation	1131:1145	arg1	effects					1147:1153	superior pro-coagulation effects	1122:1153	superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL)	1122:1227	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	9	69	theme	damaged	1679:1685	arg1	skin					1687:1690	damaged skin	1679:1690	damaged skin	1679:1690	In addition, the release of DFO enhanced the secretion of HIF-1α, further strengthening vascularization in damaged skin.
35062972	7	70	theme	coagulation	1381:1391	arg1	pathways					1393:1400	endogenous coagulation pathways	1370:1400	endogenous coagulation pathways	1370:1400	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	3	71	theme	amide	512:516	arg1	bond					518:521	an amide bond	509:521	an amide bond	509:521	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	11	72	theme	vascularization-promoting	1938:1962	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	72	theme	vascularization-promoting	1938:1962	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	3	73	theme	rapid	593:597	arg1	absorption					605:614	rapid blood absorption	593:614	rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET)	593:690	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	4	74	from	hemostasis	814:823	arg1	tissue					856:861	damaged tissue	848:861	damaged tissue	848:861	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	6	75	theme	hemostatic	1204:1213	arg1	sponges					1215:1221	clinically used collagen hemostatic sponges	1179:1221	clinically used collagen hemostatic sponges (COL)	1179:1227	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	6	75	theme	hemostatic	1204:1213	arg1	COL					1224:1226	COL	1224:1226	COL	1224:1226	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	3	76	theme	high	627:630	arg1	area					643:646	the high total pore area	623:646	the high total pore area (approximately 42.429 m2/g measured by BET)	623:690	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	7	77	theme	endogenous	1370:1379	arg1	pathways					1393:1400	endogenous coagulation pathways	1370:1400	endogenous coagulation pathways	1370:1400	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	6	78	theme	used	1190:1193	arg1	sponges					1215:1221	clinically used collagen hemostatic sponges	1179:1221	clinically used collagen hemostatic sponges (COL)	1179:1227	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	6	78	theme	used	1190:1193	arg1	COL					1224:1226	COL	1224:1226	COL	1224:1226	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	4	79	theme	DFO	716:718	arg1	release					705:711	The further release	693:711	The further release of DFO	693:718	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	11	80	theme	hemostatic	1923:1932	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	80	theme	hemostatic	1923:1932	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	7	81	theme	clotting	1329:1336	arg1	time					1338:1341	whole blood clotting time	1317:1341	whole blood clotting time	1317:1341	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	2	82	theme	sponge	303:308	arg1	OBNC-DFO					310:317	a hemostatic sponge OBNC-DFO	290:317	a hemostatic sponge OBNC-DFO	290:317	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	10	83	theme	skin	1901:1904	arg1	regeneration					1881:1892	structural and functional regeneration	1855:1892	structural and functional regeneration of the skin	1855:1904	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	7	84	theme	shorter	1309:1315	arg1	activation					1356:1365	shorter whole blood clotting time, more robust activation	1309:1365	shorter whole blood clotting time, more robust activation of endogenous coagulation pathways	1309:1400	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	8	85	theme	human	1523:1527	arg1	HUVECs					1563:1568	HUVECs	1563:1568	HUVECs	1563:1568	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	8	85	theme	human	1523:1527	arg1	cells					1556:1560	human umbilical vein endothelial cells	1523:1560	human umbilical vein endothelial cells (HUVECs)	1523:1569	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	2	86	theme	bacterial	412:420	arg1	OBNC					437:440	OBNC	437:440	OBNC	437:440	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	2	86	theme	bacterial	412:420	arg1	nanocellulose					422:434	oxidized bacterial nanocellulose	403:434	oxidized bacterial nanocellulose (OBNC)	403:441	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	4	87	from	vascularization	829:843	arg1	tissue					856:861	damaged tissue	848:861	damaged tissue	848:861	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	5	88	theme	approximate	921:931	arg1	1.70 g/s					933:940	approximate 1.70 g/s	921:940	approximate 1.70 g/s	921:940	This new hemostatic sponge can absorb water at a rate of approximate 1.70 g/s, rapidly enhancing clot formation in the early stage of hemostasis.
35062972	7	89	theme	platelets	1294:1302	arg1	activation					1260:1269	activation	1260:1269	activation of red blood cells and platelets	1260:1302	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	7	89	theme	platelets	1294:1302	arg1	aggregation					1244:1254	aggregation	1244:1254	aggregation	1244:1254	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	8	90	theme	vein	1539:1542	arg1	HUVECs					1563:1568	HUVECs	1563:1568	HUVECs	1563:1568	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	8	90	theme	vein	1539:1542	arg1	cells					1556:1560	human umbilical vein endothelial cells	1523:1560	human umbilical vein endothelial cells (HUVECs)	1523:1569	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	3	91	theme	angiogenetic	449:460	arg1	DFO					484:486	DFO	484:486	DFO	484:486	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	3	91	theme	angiogenetic	449:460	arg1	desferrioxamine					467:481	Then angiogenetic drug desferrioxamine	444:481	Then angiogenetic drug desferrioxamine (DFO)	444:487	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	1	92	theme	essential	111:119	arg1	BACKGROUND					70:79	BACKGROUND Hemostasis and repair	70:101	BACKGROUND Hemostasis and repair	70:101	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	1	92	theme	essential	111:119	arg1	processes					121:129	two essential processes	107:129	two essential processes in wound healing	107:146	BACKGROUND Hemostasis and repair are two essential processes in wound healing, yet early hemostasis and following vascularization are challenging to address in an integrated manner.
35062972	6	93	theme	coagulation	1031:1041	arg1	experiments					1043:1053	In vitro and in vivo coagulation experiments	1010:1053	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model)	1010:1107	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	8	94	theme	cells	1556:1560	arg1	proliferation					1506:1518	the proliferation	1502:1518	the proliferation of human umbilical vein endothelial cells (HUVECs)	1502:1569	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	5	95	theme	hemostasis	998:1007	arg1	stage					989:993	the early stage	979:993	the early stage of hemostasis	979:1007	This new hemostatic sponge can absorb water at a rate of approximate 1.70 g/s, rapidly enhancing clot formation in the early stage of hemostasis.
35062972	7	96	theme	red	1274:1276	arg1	cells					1284:1288	red blood cells	1274:1288	red blood cells	1274:1288	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	6	97	theme	rat	1059:1061	arg1	model					1079:1083	rat tail amputation model	1059:1083	rat tail amputation model	1059:1083	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	3	98	theme	pore	638:641	arg1	area					643:646	the high total pore area	623:646	the high total pore area (approximately 42.429 m2/g measured by BET)	623:690	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	7	99	dep	shorter	1309:1315	arg1	time					1338:1341	whole blood clotting time	1317:1341	whole blood clotting time	1317:1341	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	7	99	dep	shorter	1309:1315	arg1	robust					1349:1354	robust	1349:1354	robust	1349:1354	They promoted aggregation and activation of red blood cells and platelets with shorter whole blood clotting time, more robust activation of endogenous coagulation pathways and less blood loss.
35062972	2	100	theme	xylinus	355:361	arg1	fermentation					322:333	fermentation	322:333	fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC)	322:441	RESULTS In this study, we constructed a hemostatic sponge OBNC-DFO by fermentation of Komagataeibacter xylinus combined with TEMPO oxidation to obtain oxidized bacterial nanocellulose (OBNC).
35062972	8	101	dep	In	1423:1424	arg1	vitro					1426:1430	vitro	1426:1430	vitro	1426:1430	In vitro cellular assays showed that OBNC-DFO prevailed over OBNC by promoting the proliferation of human umbilical vein endothelial cells (HUVECs).
35062972	10	102	theme	skin	1766:1769	arg1	follicles					1794:1802	hair follicles	1789:1802	hair follicles	1789:1802	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	10	102	theme	skin	1766:1769	arg1	appendages					1771:1780	skin appendages	1766:1780	skin appendages (e.g., hair follicles)	1766:1803	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	10	102	theme	skin	1766:1769	arg1	OBNC-DFO					1756:1763	OBNC-DFO	1756:1763	OBNC-DFO	1756:1763	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
35062972	6	103	theme	trauma	1095:1100	arg1	model					1102:1106	liver trauma model	1089:1106	liver trauma model	1089:1106	In vitro and in vivo coagulation experiments (in rat tail amputation model and liver trauma model) demonstrated superior pro-coagulation effects of OBNC and OBNC-DFO to clinically used collagen hemostatic sponges (COL).
35062972	11	104	theme	nanocellulose	1983:1995	arg1	sponge					2008:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge	1918:2013	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	11	104	theme	nanocellulose	1983:1995	arg1	biomaterial					2153:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	a highly promising hemostatic and pro-regenerative repair biomaterial	2095:2163	CONCLUSION This hemostatic and vascularization-promoting oxidized bacterial nanocellulose hemostatic sponge, which rapidly activates coagulation pathways and enables skin regeneration, is a highly promising hemostatic and pro-regenerative repair biomaterial.
35062972	4	105	theme	blood	781:785	arg1	flow					787:790	blood flow	781:790	blood flow	781:790	The further release of DFO stimulated the secretion of HIF-1α and the reconstruction of blood flow, thus achieving rapid hemostasis and vascularization in damaged tissue.
35062972	3	106	dep	area	643:646	arg1	42.429 m2/g					663:673	approximately 42.429 m2/g	649:673	approximately 42.429 m2/g measured by BET	649:689	Then angiogenetic drug desferrioxamine (DFO) was grafted through an amide bond, and it promoted clot formation and activated coagulation reaction by rapid blood absorption due to the high total pore area (approximately 42.429 m2/g measured by BET).
35062972	9	107	theme	DFO	1600:1602	arg1	release					1589:1595	the release	1585:1595	the release of DFO	1585:1602	In addition, the release of DFO enhanced the secretion of HIF-1α, further strengthening vascularization in damaged skin.
35062972	10	108	theme	rat	1700:1702	arg1	model					1716:1720	the rat skin injury model	1696:1720	the rat skin injury model	1696:1720	In the rat skin injury model, 28 days after being treated with OBNC-DFO, skin appendages (e.g., hair follicles) became more intact, indicating the achievement of structural and functional regeneration of the skin.
36145082	0	0	theme	Gut	79:81	arg1	Composition					94:104	Gut Microbiota Composition	79:104	Gut Microbiota Composition	79:104	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.
36145082	4	1	theme	antioxidant-related	902:920	arg1	gene					922:925	antioxidant-related gene	902:925	antioxidant-related gene (GPX1 and GPX4) expression	902:952	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	2	theme	Asp	628:630	arg1	administration					632:645	Asp administration	628:645	Asp administration	628:645	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	3	from	peroxidase	724:733	arg1	tissue					813:818	the colonic tissue	801:818	the colonic tissue	801:818	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	2	4	theme	energy	308:313	arg1	Aspartate					276:284	Aspartate	276:284	Aspartate (Asp)	276:290	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	2	4	theme	energy	308:313	arg1	source					315:320	an essential energy source	295:320	an essential energy source	295:320	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	4	5	theme	DSS-treated	957:967	arg1	mice					969:972	DSS-treated mice	957:972	DSS-treated mice	957:972	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	5	6	theme	MLKL	1106:1109	arg1	expression					1119:1128	the MLKL protein expression	1102:1128	the MLKL protein expression	1102:1128	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	2	7	theme	essential	298:306	arg1	Aspartate					276:284	Aspartate	276:284	Aspartate (Asp)	276:290	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	2	7	theme	essential	298:306	arg1	source					315:320	an essential energy source	295:320	an essential energy source	295:320	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	6	8	theme	phylum	1290:1295	arg1	level					1297:1301	the phylum level	1286:1301	the phylum level	1286:1301	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	4	9	theme	colonic	805:811	arg1	tissue					813:818	the colonic tissue	801:818	the colonic tissue	801:818	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	0	10	from	Improvement	0:10	arg1	Mice					109:112	Mice	109:112	Mice	109:112	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.
36145082	5	11	from	administration	1054:1067	arg1	mice					1088:1091	the DSS-induced mice	1072:1091	the DSS-induced mice	1072:1091	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	3	12	theme	DSS-induced	475:485	arg1	colitis					487:493	DSS-induced colitis	475:493	DSS-induced colitis	475:493	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	1	13	theme	intestinal	227:236	arg1	mucosa					238:243	the intestinal mucosa	223:243	the intestinal mucosa	223:243	The intestine requires a great deal of energy to maintain its health and function; thus, energy deficits in the intestinal mucosa may lead to intestinal damage.
36145082	0	14	theme	Microbiota	83:92	arg1	Composition					94:104	Gut Microbiota Composition	79:104	Gut Microbiota Composition	79:104	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.
36145082	5	15	theme	RIPK3	1006:1010	arg1	levels					986:991	Expression levels	975:991	Expression levels of RIPK1 and RIPK3	975:1010	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	4	16	dep	gene	922:925	arg1	GPX1					928:931	GPX1	928:931	GPX1	928:931	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	16	dep	gene	922:925	arg1	GPX4					937:940	GPX4	937:940	GPX4	937:940	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	17	from	myeloperoxidase	696:710	arg1	tissue					813:818	the colonic tissue	801:818	the colonic tissue	801:818	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	3	18	theme	dietary	428:434	arg1	supplementation					436:450	dietary supplementation	428:450	dietary supplementation of Asp	428:457	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	7	19	theme	antioxidative	1484:1496	arg1	capacity					1498:1505	antioxidative capacity	1484:1505	antioxidative capacity	1484:1505	Asp may positively regulate the recovery of DSS-induced damage by improving the immunity and antioxidative capacity, regulating RIPK signaling and modulating the gut microbiota composition.
36145082	4	20	theme	colonic	878:884	arg1	morphology					886:895	colonic morphology	878:895	colonic morphology	878:895	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	21	from	concentrations	783:796	arg1	tissue					813:818	the colonic tissue	801:818	the colonic tissue	801:818	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	6	22	theme	Asp	1177:1179	arg1	treatment					1181:1189	Asp treatment	1177:1189	Asp treatment	1177:1189	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	6	23	theme	phylum	1377:1382	arg1	level					1384:1388	the phylum level	1373:1388	the phylum level	1373:1388	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	4	24	theme	eosinophil	713:722	arg1	peroxidase					724:733	eosinophil peroxidase	713:733	eosinophil peroxidase	713:733	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	1	25	theme	energy	154:159	arg1	deal					146:149	a great deal	138:149	a great deal of energy	138:159	The intestine requires a great deal of energy to maintain its health and function; thus, energy deficits in the intestinal mucosa may lead to intestinal damage.
36145082	4	26	from	apoptosis	685:693	arg1	tissue					813:818	the colonic tissue	801:818	the colonic tissue	801:818	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	6	27	theme	Verrucomicrobia	1354:1368	arg1	abundance					1322:1330	the abundance	1318:1330	the abundance of Actinobacteria and Verrucomicrobia	1318:1368	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	7	28	theme	microbiota	1557:1566	arg1	composition					1568:1578	the gut microbiota composition	1549:1578	the gut microbiota composition	1549:1578	Asp may positively regulate the recovery of DSS-induced damage by improving the immunity and antioxidative capacity, regulating RIPK signaling and modulating the gut microbiota composition.
36145082	6	29	theme	16S	1145:1147	arg1	sequencing					1154:1163	16S rRNA sequencing	1145:1163	16S rRNA sequencing	1145:1163	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	5	30	theme	DSS-induced	1076:1086	arg1	mice					1088:1091	the DSS-induced mice	1072:1091	the DSS-induced mice	1072:1091	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	2	31	theme	intestinal	329:338	arg1	mucosa					340:345	the intestinal mucosa	325:345	the intestinal mucosa	325:345	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	4	32	from	intake	870:875	arg1	mice					969:972	DSS-treated mice	957:972	DSS-treated mice	957:972	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	33	from	index	678:682	arg1	tissue					813:818	the colonic tissue	801:818	the colonic tissue	801:818	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	34	theme	activity	669:676	arg1	index					678:682	the disease activity index	657:682	the disease activity index	657:682	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	0	35	theme	Colitis	26:32	arg1	Improvement					0:10	Improvement	0:10	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.	0:113	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.
36145082	4	36	from	expression	943:952	arg1	mice					969:972	DSS-treated mice	957:972	DSS-treated mice	957:972	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	2	37	from	source	315:320	arg1	mucosa					340:345	the intestinal mucosa	325:345	the intestinal mucosa	325:345	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	3	38	theme	current	392:398	arg1	study					400:404	the current study	388:404	the current study	388:404	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	0	39	theme	Ulcerative	15:24	arg1	Colitis					26:32	Ulcerative Colitis	15:32	Ulcerative Colitis	15:32	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.
36145082	7	40	theme	gut	1553:1555	arg1	composition					1568:1578	the gut microbiota composition	1549:1578	the gut microbiota composition	1549:1578	Asp may positively regulate the recovery of DSS-induced damage by improving the immunity and antioxidative capacity, regulating RIPK signaling and modulating the gut microbiota composition.
36145082	6	41	theme	gene	1253:1256	arg1	level					1258:1262	the gene level	1249:1262	the gene level	1249:1262	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	3	42	theme	microbiota	576:585	arg1	composition					587:597	microbiota composition	576:597	microbiota composition	576:597	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	2	43	theme	vital	359:363	arg1	part					365:368	a vital part	357:368	a vital part	357:368	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	5	44	theme	Asp	1050:1052	arg1	administration					1054:1067	Asp administration	1050:1067	Asp administration in the DSS-induced mice	1050:1091	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	1	45	theme	intestinal	257:266	arg1	damage					268:273	intestinal damage	257:273	intestinal damage	257:273	The intestine requires a great deal of energy to maintain its health and function; thus, energy deficits in the intestinal mucosa may lead to intestinal damage.
36145082	4	46	theme	daily	859:863	arg1	intake					870:875	average daily food intake	851:875	average daily food intake	851:875	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	6	47	theme	rRNA	1149:1152	arg1	sequencing					1154:1163	16S rRNA sequencing	1145:1163	16S rRNA sequencing	1145:1163	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	5	48	theme	protein	1111:1117	arg1	expression					1119:1128	the MLKL protein expression	1102:1128	the MLKL protein expression	1102:1128	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	4	49	theme	gene	922:925	arg1	expression					943:952	antioxidant-related gene (GPX1 and GPX4) expression	902:952	antioxidant-related gene (GPX1 and GPX4) expression	902:952	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	5	50	dep	increased	1017:1025	arg1	whereas					1094:1100	whereas	1094:1100	whereas	1094:1100	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	4	51	theme	average	851:857	arg1	intake					870:875	average daily food intake	851:875	average daily food intake	851:875	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	5	52	theme	RIPK1	996:1000	arg1	levels					986:991	Expression levels	975:991	Expression levels of RIPK1 and RIPK3	975:1010	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	2	53	theme	gut	373:375	arg1	health					377:382	gut health	373:382	gut health	373:382	Aspartate (Asp) is an essential energy source in the intestinal mucosa and plays a vital part in gut health.
36145082	4	54	dep	cytokine	756:763	arg1	TNF-α					776:780	TNF-α	776:780	TNF-α	776:780	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	54	dep	cytokine	756:763	arg1	IL-1β					766:770	IL-1β	766:770	IL-1β	766:770	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	0	55	theme	RIPK	51:54	arg1	Modulation					64:73	RIPK Pathway Modulation	51:73	RIPK Pathway Modulation	51:73	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.
36145082	7	56	theme	damage	1447:1452	arg1	recovery					1423:1430	the recovery	1419:1430	the recovery of DSS-induced damage	1419:1452	Asp may positively regulate the recovery of DSS-induced damage by improving the immunity and antioxidative capacity, regulating RIPK signaling and modulating the gut microbiota composition.
36145082	6	57	theme	Alistipes	1236:1244	arg1	abundance					1205:1213	the abundance	1201:1213	the abundance of Lactobacillus and Alistipes	1201:1244	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	4	58	from	weight	843:848	arg1	mice					969:972	DSS-treated mice	957:972	DSS-treated mice	957:972	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	3	59	theme	colonic	518:524	arg1	morphology					526:535	the colonic morphology	514:535	the colonic morphology	514:535	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	6	60	theme	Lactobacillus	1218:1230	arg1	abundance					1205:1213	the abundance	1201:1213	the abundance of Lactobacillus and Alistipes	1201:1244	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	3	61	theme	dextran	619:625	arg1	model					610:614	a mouse model	602:614	a mouse model of dextran	602:625	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	1	62	from	deficits	211:218	arg1	mucosa					238:243	the intestinal mucosa	223:243	the intestinal mucosa	223:243	The intestine requires a great deal of energy to maintain its health and function; thus, energy deficits in the intestinal mucosa may lead to intestinal damage.
36145082	3	63	theme	mouse	604:608	arg1	model					610:614	a mouse model	602:614	a mouse model of dextran	602:625	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	7	64	theme	DSS-induced	1435:1445	arg1	damage					1447:1452	DSS-induced damage	1435:1452	DSS-induced damage	1435:1452	Asp may positively regulate the recovery of DSS-induced damage by improving the immunity and antioxidative capacity, regulating RIPK signaling and modulating the gut microbiota composition.
36145082	4	65	theme	cytokine	756:763	arg1	concentrations					783:796	proinflammatory cytokine (IL-1β and TNF-α) concentrations	740:796	proinflammatory cytokine (IL-1β and TNF-α) concentrations	740:796	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	4	66	theme	body	838:841	arg1	weight					843:848	the body weight	834:848	the body weight	834:848	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	3	67	theme	cell	556:559	arg1	apoptosis					561:569	cell apoptosis	556:569	cell apoptosis	556:569	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	3	68	theme	oxidative	538:546	arg1	stress					548:553	oxidative stress	538:553	oxidative stress	538:553	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	4	69	theme	disease	661:667	arg1	index					678:682	the disease activity index	657:682	the disease activity index	657:682	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	3	70	from	improvement	499:509	arg1	composition					587:597	microbiota composition	576:597	microbiota composition	576:597	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	3	70	from	improvement	499:509	arg1	apoptosis					561:569	cell apoptosis	556:569	cell apoptosis	556:569	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	3	70	from	improvement	499:509	arg1	morphology					526:535	the colonic morphology	514:535	the colonic morphology	514:535	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	3	70	from	improvement	499:509	arg1	stress					548:553	oxidative stress	538:553	oxidative stress	538:553	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	3	70	from	improvement	499:509	arg1	model					610:614	a mouse model	602:614	a mouse model of dextran	602:625	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	6	71	theme	Actinobacteria	1335:1348	arg1	abundance					1322:1330	the abundance	1318:1330	the abundance of Actinobacteria and Verrucomicrobia	1318:1368	16S rRNA sequencing showed that Asp treatment increased the abundance of Lactobacillus and Alistipes at the gene level, and Bacteroidetes at the phylum level, but decreased the abundance of Actinobacteria and Verrucomicrobia at the phylum level.
36145082	3	72	theme	Asp	455:457	arg1	supplementation					436:450	dietary supplementation	428:450	dietary supplementation of Asp	428:457	In the current study, we hypothesized that dietary supplementation of Asp could alleviate DSS-induced colitis via improvement in the colonic morphology, oxidative stress, cell apoptosis, and microbiota composition in a mouse model of dextran.
36145082	7	73	theme	RIPK	1519:1522	arg1	signaling					1524:1532	RIPK signaling	1519:1532	RIPK signaling	1519:1532	Asp may positively regulate the recovery of DSS-induced damage by improving the immunity and antioxidative capacity, regulating RIPK signaling and modulating the gut microbiota composition.
36145082	5	74	theme	Expression	975:984	arg1	levels					986:991	Expression levels	975:991	Expression levels of RIPK1 and RIPK3	975:1010	Expression levels of RIPK1 and RIPK3 were increased in the colon following Asp administration in the DSS-induced mice, whereas the MLKL protein expression was decreased.
36145082	4	75	theme	proinflammatory	740:754	arg1	cytokine					756:763	proinflammatory cytokine	740:763	proinflammatory cytokine (IL-1β and TNF-α) concentrations	740:796	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	1	76	theme	energy	204:209	arg1	deficits					211:218	energy deficits	204:218	energy deficits in the intestinal mucosa	204:243	The intestine requires a great deal of energy to maintain its health and function; thus, energy deficits in the intestinal mucosa may lead to intestinal damage.
36145082	0	77	theme	Pathway	56:62	arg1	Modulation					64:73	RIPK Pathway Modulation	51:73	RIPK Pathway Modulation	51:73	Improvement of Ulcerative Colitis by Aspartate via RIPK Pathway Modulation and Gut Microbiota Composition in Mice.
36145082	4	78	from	morphology	886:895	arg1	mice					969:972	DSS-treated mice	957:972	DSS-treated mice	957:972	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
36145082	1	79	theme	great	140:144	arg1	deal					146:149	a great deal	138:149	a great deal of energy	138:159	The intestine requires a great deal of energy to maintain its health and function; thus, energy deficits in the intestinal mucosa may lead to intestinal damage.
36145082	4	80	theme	food	865:868	arg1	intake					870:875	average daily food intake	851:875	average daily food intake	851:875	Asp administration decreased the disease activity index, apoptosis, myeloperoxidase, eosinophil peroxidase, and proinflammatory cytokine (IL-1β and TNF-α) concentrations in the colonic tissue, but improved the body weight, average daily food intake, colonic morphology, and antioxidant-related gene (GPX1 and GPX4) expression in DSS-treated mice.
37315646	14	0	theme	CHQW	2501:2504	arg1	related					2513:2519	related	2513:2519	related	2513:2519	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	14	0	theme	CHQW	2501:2504	arg1	mechanism					2488:2496	The protective mechanism	2473:2496	The protective mechanism of CHQW	2473:2504	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	10	1	dep	cytokines	1728:1736	arg1	interleukin-17					1755:1768	interleukin-17	1755:1768	interleukin-17	1755:1768	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	1	dep	cytokines	1728:1736	arg1	interleukin-1β					1739:1752	interleukin-1β	1739:1752	interleukin-1β	1739:1752	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	1	dep	cytokines	1728:1736	arg1	factor-α					1789:1796	tumor necrosis factor-α	1774:1796	tumor necrosis factor-α	1774:1796	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	1	dep	cytokines	1728:1736	arg1	cytokines					1728:1736	inflammatory cytokines	1715:1736	inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α)	1715:1797	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	2	2	theme	diseases	347:354	arg1	treatment					322:330	the treatment	318:330	the treatment of respiratory diseases	318:354	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	9	3	theme	CHQW	1487:1490	arg1	composition					1472:1482	The chemical composition	1459:1482	The chemical composition of CHQW	1459:1490	The chemical composition of CHQW was identified by liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
37315646	11	4	theme	p-IκBα	1870:1875	arg1	expression					1850:1859	the expression	1846:1859	the expression of TLR4, p-IκBα and NF-κB proteins	1846:1894	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	15	5	theme	active	2606:2611	arg1	flavonoids					2637:2646	flavonoids	2637:2646	flavonoids	2637:2646	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	15	5	theme	active	2606:2611	arg1	ingredients					2613:2623	The main active ingredients	2597:2623	The main active ingredients of CHQW	2597:2631	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	13	6	theme	inflammatory	2422:2433	arg1	cytokines					2435:2443	inflammatory cytokines	2422:2443	inflammatory cytokines released in BALF and serum	2422:2470	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	9	7	theme	liquid	1510:1515	arg1	chromatography-quadrupole-time					1517:1546	liquid chromatography-quadrupole-time	1510:1546	liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS)	1510:1588	The chemical composition of CHQW was identified by liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
37315646	8	8	contain	containing	1344:1353	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	8	contain	containing	1344:1353	arg1	receptor					1209:1216	toll-like receptor 4	1199:1218	toll-like receptor 4 (TLR4)	1199:1225	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	8	contain	containing	1344:1353	arg1	B					1305:1305	nuclear-factor-kappa B	1284:1305	nuclear-factor-kappa B (NF-κB)	1284:1313	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	8	contain	containing	1344:1353	arg2	levels					1375:1380	3(NLRP3) expression levels	1355:1380	3(NLRP3) expression levels	1355:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	8	contain	containing	1344:1353	arg1	phospho-IκBα					1261:1272	phospho-IκBα (p-IκBα)	1261:1281	phospho-IκBα (p-IκBα)	1261:1281	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	8	contain	containing	1344:1353	arg1	alpha					1247:1251	inhibitory kappa B alpha	1228:1251	inhibitory kappa B alpha (IκBα)	1228:1258	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	8	contain	containing	1344:1353	arg1	domain					1337:1342	NLR family pyrin domain	1320:1342	NLR family pyrin domain	1320:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	13	9	dep	CONCLUSION	2245:2254	arg1	showed					2282:2287	showed	2282:2287	showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum	2282:2470	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	13	10	from	ALI	2365:2367	arg1	rats					2372:2375	rats	2372:2375	rats	2372:2375	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	8	11	theme	nuclear-factor-kappa	1284:1303	arg1	NF-κB					1308:1312	NF-κB	1308:1312	NF-κB	1308:1312	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	11	theme	nuclear-factor-kappa	1284:1303	arg1	B					1305:1305	nuclear-factor-kappa B	1284:1305	nuclear-factor-kappa B (NF-κB)	1284:1313	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	11	theme	nuclear-factor-kappa	1284:1303	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	10	12	theme	tissue	1635:1640	arg1	injury					1655:1660	lung tissue pathological injury	1630:1660	lung tissue pathological injury	1630:1660	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	1	13	theme	severe	210:215	arg1	symptoms					217:224	severe symptoms	210:224	severe symptoms	210:224	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	9	14	theme	spectrometry	1563:1574	arg1	chromatography-quadrupole-time					1517:1546	liquid chromatography-quadrupole-time	1510:1546	liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS)	1510:1588	The chemical composition of CHQW was identified by liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
37315646	13	15	contain	had	2319:2321	arg2	effect					2343:2348	a strong protective effect	2323:2348	a strong protective effect	2323:2348	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	13	15	contain	had	2319:2321	arg1	pretreatment					2298:2309	the pretreatment	2294:2309	the pretreatment of CHQW	2294:2317	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	13	16	theme	protective	2332:2341	arg1	effect					2343:2348	a strong protective effect	2323:2348	a strong protective effect	2323:2348	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	4	17	from	effect	531:536	arg1	model					606:610	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model	549:610	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats	549:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	4	18	theme	anti-inflammatory	513:529	arg1	effect					531:536	the anti-inflammatory effect	509:536	the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats	509:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	10	19	theme	LPS-induced	1665:1675	arg1	rats					1681:1684	LPS-induced ALI rats	1665:1684	LPS-induced ALI rats	1665:1684	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	11	20	theme	signaling	1951:1959	arg1	pathway					1961:1967	the TLR4/NF-κB signaling pathway	1936:1967	the TLR4/NF-κB signaling pathway	1936:1967	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	7	21	theme	histopathological	1012:1028	arg1	changes					1030:1036	The histopathological changes	1008:1036	The histopathological changes in the lung	1008:1048	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	13	22	theme	CHQW	2314:2317	arg1	pretreatment					2298:2309	the pretreatment	2294:2309	the pretreatment of CHQW	2294:2317	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	2	23	theme	Qingwen	269:275	arg1	formula					306:312	a classic formula	296:312	a classic formula for the treatment of respiratory diseases	296:354	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	2	23	theme	Qingwen	269:275	arg1	CHQW					287:290	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	5	24	theme	Qingwen	838:844	arg1	group					854:858	the Lianhua Qingwen capsule group	826:858	the Lianhua Qingwen capsule group	826:858	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	8	25	theme	pyrin	1331:1335	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	25	theme	pyrin	1331:1335	arg1	domain					1337:1342	NLR family pyrin domain	1320:1342	NLR family pyrin domain	1320:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	7	26	from	fluid	1115:1119	arg1	changes					1030:1036	The histopathological changes	1008:1036	The histopathological changes in the lung	1008:1048	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	26	from	fluid	1115:1119	arg1	levels					1058:1063	the levels	1054:1063	the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats	1054:1148	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	14	27	theme	signaling	2557:2565	arg1	pathway					2567:2573	the TLR4/NF-κB signaling pathway	2542:2573	the TLR4/NF-κB signaling pathway	2542:2573	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	0	28	theme	granules	66:73	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect and component analysis of Chaihu Qingwen granules.
37315646	0	28	theme	granules	66:73	arg1	analysis					39:46	component analysis	29:46	component analysis of Chaihu Qingwen granules	29:73	Anti-inflammatory effect and component analysis of Chaihu Qingwen granules.
37315646	12	29	theme	organic	2181:2187	arg1	information					2129:2139	information	2129:2139	information from the literature	2129:2159	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	12	29	theme	organic	2181:2187	arg1	acids					2189:2193	organic acids	2181:2193	organic acids	2181:2193	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	1	30	theme	acute	170:174	arg1	injury					181:186	acute lung injury	170:186	acute lung injury	170:186	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	13	31	dep	had	2319:2321	arg1	decreasing					2411:2420	decreasing	2411:2420	decreasing inflammatory cytokines released in BALF and serum	2411:2470	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	13	31	dep	had	2319:2321	arg1	reducing					2378:2385	reducing	2378:2385	reducing lung tissue lesions	2378:2405	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	4	32	theme	potential	639:647	arg1	mechanism					649:657	its potential mechanism	635:657	its potential mechanism	635:657	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	5	33	theme	MATERIALS	709:717	arg1	rats					739:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	12	34	from	literature	2150:2159	arg1	acids					2189:2193	organic acids	2181:2193	organic acids	2181:2193	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	12	34	from	literature	2150:2159	arg1	glycosides					2233:2242	phenylethanoid glycosides	2218:2242	phenylethanoid glycosides	2218:2242	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	12	34	from	literature	2150:2159	arg1	lignans					2196:2202	lignans	2196:2202	lignans	2196:2202	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	12	34	from	literature	2150:2159	arg1	iridoids					2205:2212	iridoids	2205:2212	iridoids	2205:2212	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	12	34	from	literature	2150:2159	arg1	information					2129:2139	information	2129:2139	information from the literature	2129:2159	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	12	34	from	literature	2150:2159	arg1	flavonoids					2169:2178	flavonoids	2169:2178	flavonoids	2169:2178	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	3	35	theme	Clinical	357:364	arg1	observation					366:376	Clinical observation	357:376	Clinical observation	357:376	Clinical observation shows that CHQW has good efficacy in treating colds, coughs, and fevers.
37315646	8	36	theme	family	1324:1329	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	36	theme	family	1324:1329	arg1	domain					1337:1342	NLR family pyrin domain	1320:1342	NLR family pyrin domain	1320:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	7	37	theme	factors	1081:1087	arg1	changes					1030:1036	The histopathological changes	1008:1036	The histopathological changes in the lung	1008:1048	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	37	theme	factors	1081:1087	arg1	levels					1058:1063	the levels	1054:1063	the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats	1054:1148	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	13	38	theme	study	2276:2280	arg1	results					2260:2266	The results	2256:2266	The results of this study	2256:2280	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	14	39	theme	TLR4/NF-κB	2546:2555	arg1	pathway					2567:2573	the TLR4/NF-κB signaling pathway	2542:2573	the TLR4/NF-κB signaling pathway	2542:2573	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	14	40	theme	NLRP3	2579:2583	arg1	activation					2585:2594	NLRP3 activation	2579:2594	NLRP3 activation	2579:2594	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	4	41	theme	STUDY	462:466	arg1	AIM					451:453	AIM	451:453	AIM OF THE STUDY	451:466	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	8	42	theme	inhibitory	1228:1237	arg1	IκBα					1254:1257	IκBα	1254:1257	IκBα	1254:1257	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	42	theme	inhibitory	1228:1237	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	42	theme	inhibitory	1228:1237	arg1	alpha					1247:1251	inhibitory kappa B alpha	1228:1251	inhibitory kappa B alpha (IκBα)	1228:1258	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	0	43	theme	Anti-inflammatory	0:16	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect and component analysis of Chaihu Qingwen granules.
37315646	1	44	theme	prevalent	110:118	arg1	condition					138:146	prevalent acute respiratory condition	110:146	prevalent acute respiratory condition	110:146	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	6	45	theme	injury	939:944	arg1	model					952:956	The LPS-induced acute lung injury (ALI) model	912:956	The LPS-induced acute lung injury (ALI) model in rats	912:964	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	11	46	theme	NLRP3	2002:2006	arg1	activation					1988:1997	the activation	1984:1997	the activation of NLRP3	1984:2006	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	8	47	theme	NLRP3	1357:1361	arg1	levels					1375:1380	3(NLRP3) expression levels	1355:1380	3(NLRP3) expression levels	1355:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	48	theme	B	1245:1245	arg1	IκBα					1254:1257	IκBα	1254:1257	IκBα	1254:1257	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	48	theme	B	1245:1245	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	48	theme	B	1245:1245	arg1	alpha					1247:1251	inhibitory kappa B alpha	1228:1251	inhibitory kappa B alpha (IκBα)	1228:1258	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	1	49	theme	respiratory	126:136	arg1	condition					138:146	prevalent acute respiratory condition	110:146	prevalent acute respiratory condition	110:146	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	6	50	theme	acute	928:932	arg1	ALI					947:949	ALI	947:949	ALI	947:949	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	6	50	theme	acute	928:932	arg1	injury					939:944	LPS-induced acute lung injury	916:944	The LPS-induced acute lung injury (ALI) model in rats	912:964	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	8	51	theme	expression	1364:1373	arg1	levels					1375:1380	3(NLRP3) expression levels	1355:1380	3(NLRP3) expression levels	1355:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	5	52	theme	METHODS	723:729	arg1	rats					739:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	13	53	theme	tissue	2392:2397	arg1	lesions					2399:2405	lung tissue lesions	2387:2405	lung tissue lesions	2387:2405	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	3	54	theme	good	398:401	arg1	efficacy					403:410	good efficacy	398:410	good efficacy	398:410	Clinical observation shows that CHQW has good efficacy in treating colds, coughs, and fevers.
37315646	1	55	contain	has	188:190	arg2	symptoms					217:224	severe symptoms	210:224	severe symptoms	210:224	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	1	55	contain	has	188:190	arg1	RELEVANCE					97:105	ETHNOPHARMACOLOGICAL RELEVANCE	76:105	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice	76:167	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	1	55	contain	has	188:190	arg2	start					200:204	a quick start	192:204	a quick start	192:204	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	1	55	contain	has	188:190	arg1	injury					181:186	acute lung injury	170:186	acute lung injury	170:186	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	2	56	theme	respiratory	335:345	arg1	diseases					347:354	respiratory diseases	335:354	respiratory diseases	335:354	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	5	57	theme	SD	736:737	arg1	rats					739:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	8	58	theme	blotting	1407:1414	arg1	analysis					1416:1423	western blotting analysis	1399:1423	western blotting analysis	1399:1423	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	12	59	theme	chemical	2013:2020	arg1	components					2022:2031	The chemical components	2009:2031	The chemical components of CHQW	2009:2039	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	6	60	from	model	952:956	arg1	rats					961:964	rats	961:964	rats	961:964	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	5	61	theme	Male	731:734	arg1	rats					739:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats	709:742	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	9	62	theme	chemical	1463:1470	arg1	composition					1472:1482	The chemical composition	1459:1482	The chemical composition of CHQW	1459:1490	The chemical composition of CHQW was identified by liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
37315646	4	63	theme	acute	582:586	arg1	ALI					601:603	ALI	601:603	ALI	601:603	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	4	63	theme	acute	582:586	arg1	injury					593:598	acute lung injury	582:598	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats	549:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	15	64	theme	CHQW	2628:2631	arg1	flavonoids					2637:2646	flavonoids	2637:2646	flavonoids	2637:2646	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	15	64	theme	CHQW	2628:2631	arg1	ingredients					2613:2623	The main active ingredients	2597:2623	The main active ingredients of CHQW	2597:2631	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	1	65	from	RELEVANCE	97:105	arg1	practice					160:167	clinical practice	151:167	clinical practice	151:167	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	10	66	theme	inflammatory	1715:1726	arg1	interleukin-17					1755:1768	interleukin-17	1755:1768	interleukin-17	1755:1768	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	66	theme	inflammatory	1715:1726	arg1	interleukin-1β					1739:1752	interleukin-1β	1739:1752	interleukin-1β	1739:1752	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	66	theme	inflammatory	1715:1726	arg1	cytokines					1728:1736	inflammatory cytokines	1715:1736	inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α)	1715:1797	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	66	theme	inflammatory	1715:1726	arg1	factor-α					1789:1796	tumor necrosis factor-α	1774:1796	tumor necrosis factor-α	1774:1796	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	67	theme	necrosis	1780:1787	arg1	factor-α					1789:1796	tumor necrosis factor-α	1774:1796	tumor necrosis factor-α	1774:1796	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	67	theme	necrosis	1780:1787	arg1	cytokines					1728:1736	inflammatory cytokines	1715:1736	inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α)	1715:1797	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	68	theme	lung	1630:1633	arg1	injury					1655:1660	lung tissue pathological injury	1630:1660	lung tissue pathological injury	1630:1660	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	5	69	theme	ibuprofen	809:817	arg1	group					819:823	the ibuprofen group	805:823	the ibuprofen group	805:823	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	14	70	theme	pathway	2567:2573	arg1	inhibition					2528:2537	the inhibition	2524:2537	the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation	2524:2594	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	15	71	theme	main	2601:2604	arg1	flavonoids					2637:2646	flavonoids	2637:2646	flavonoids	2637:2646	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	15	71	theme	main	2601:2604	arg1	ingredients					2613:2623	The main active ingredients	2597:2623	The main active ingredients of CHQW	2597:2631	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	11	72	theme	TLR4	1864:1867	arg1	expression					1850:1859	the expression	1846:1859	the expression of TLR4, p-IκBα and NF-κB proteins	1846:1894	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	1	73	theme	quick	194:198	arg1	start					200:204	a quick start	192:204	a quick start	192:204	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	0	74	theme	Chaihu	51:56	arg1	granules					66:73	Chaihu Qingwen granules	51:73	Chaihu Qingwen granules	51:73	Anti-inflammatory effect and component analysis of Chaihu Qingwen granules.
37315646	7	75	from	serum	1132:1136	arg1	changes					1030:1036	The histopathological changes	1008:1036	The histopathological changes in the lung	1008:1048	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	75	from	serum	1132:1136	arg1	levels					1058:1063	the levels	1054:1063	the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats	1054:1148	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	76	theme	bronchoalveolar	1092:1106	arg1	lavage					1108:1113	bronchoalveolar lavage	1092:1113	bronchoalveolar lavage fluid (BALF)	1092:1126	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	13	77	theme	LPS-induced	2353:2363	arg1	ALI					2365:2367	LPS-induced ALI	2353:2367	LPS-induced ALI in rats	2353:2375	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	4	78	from	model	606:610	arg1	rats					615:618	rats	615:618	rats	615:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	5	79	theme	model	792:796	arg1	group					798:802	the model group	788:802	the model group	788:802	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	7	80	from	changes	1030:1036	arg1	lung					1045:1048	the lung	1041:1048	the lung	1041:1048	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	80	from	changes	1030:1036	arg1	BALF					1122:1125	BALF	1122:1125	BALF	1122:1125	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	80	from	changes	1030:1036	arg1	serum					1132:1136	serum	1132:1136	serum of ALI	1132:1143	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	80	from	changes	1030:1036	arg1	fluid					1115:1119	bronchoalveolar lavage fluid	1092:1119	bronchoalveolar lavage fluid (BALF)	1092:1126	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	3	81	contain	has	394:396	arg1	CHQW					389:392	CHQW	389:392	CHQW	389:392	Clinical observation shows that CHQW has good efficacy in treating colds, coughs, and fevers.
37315646	3	81	contain	has	394:396	arg2	efficacy					403:410	good efficacy	398:410	good efficacy	398:410	Clinical observation shows that CHQW has good efficacy in treating colds, coughs, and fevers.
37315646	2	82	theme	classic	298:304	arg1	formula					306:312	a classic formula	296:312	a classic formula for the treatment of respiratory diseases	296:354	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	2	82	theme	classic	298:304	arg1	CHQW					287:290	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	9	83	theme	flight-mass	1551:1561	arg1	spectrometry					1563:1574	flight-mass spectrometry	1551:1574	flight-mass spectrometry (LC-Q-TOF-MS)	1551:1588	The chemical composition of CHQW was identified by liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
37315646	9	83	theme	flight-mass	1551:1561	arg1	LC-Q-TOF-MS					1577:1587	LC-Q-TOF-MS	1577:1587	LC-Q-TOF-MS	1577:1587	The chemical composition of CHQW was identified by liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
37315646	7	84	from	levels	1058:1063	arg1	lung					1045:1048	the lung	1041:1048	the lung	1041:1048	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	84	from	levels	1058:1063	arg1	BALF					1122:1125	BALF	1122:1125	BALF	1122:1125	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	84	from	levels	1058:1063	arg1	serum					1132:1136	serum	1132:1136	serum of ALI	1132:1143	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	84	from	levels	1058:1063	arg1	fluid					1115:1119	bronchoalveolar lavage fluid	1092:1119	bronchoalveolar lavage fluid (BALF)	1092:1126	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	15	85	theme	phenylethanoid	2686:2699	arg1	glycosides					2701:2710	phenylethanoid glycosides	2686:2710	phenylethanoid glycosides	2686:2710	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	4	86	theme	injury	593:598	arg1	model					606:610	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model	549:610	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats	549:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	13	87	theme	strong	2325:2330	arg1	effect					2343:2348	a strong protective effect	2323:2348	a strong protective effect	2323:2348	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	7	88	from	factors	1081:1087	arg1	BALF					1122:1125	BALF	1122:1125	BALF	1122:1125	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	88	from	factors	1081:1087	arg1	serum					1132:1136	serum	1132:1136	serum of ALI	1132:1143	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	88	from	factors	1081:1087	arg1	fluid					1115:1119	bronchoalveolar lavage fluid	1092:1119	bronchoalveolar lavage fluid (BALF)	1092:1126	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	5	89	theme	blank	775:779	arg1	group					781:785	the blank group	771:785	the blank group	771:785	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	12	90	theme	phenylethanoid	2218:2231	arg1	information					2129:2139	information	2129:2139	information from the literature	2129:2159	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	12	90	theme	phenylethanoid	2218:2231	arg1	glycosides					2233:2242	phenylethanoid glycosides	2218:2242	phenylethanoid glycosides	2218:2242	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	15	91	theme	organic	2649:2655	arg1	acids					2657:2661	organic acids	2649:2661	organic acids	2649:2661	The main active ingredients of CHQW are flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	14	92	theme	protective	2477:2486	arg1	related					2513:2519	related	2513:2519	related	2513:2519	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	14	92	theme	protective	2477:2486	arg1	mechanism					2488:2496	The protective mechanism	2473:2496	The protective mechanism of CHQW	2473:2504	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	10	93	theme	ALI	1677:1679	arg1	rats					1681:1684	LPS-induced ALI rats	1665:1684	LPS-induced ALI rats	1665:1684	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	11	94	theme	TLR4/NF-κB	1940:1949	arg1	pathway					1961:1967	the TLR4/NF-κB signaling pathway	1936:1967	the TLR4/NF-κB signaling pathway	1936:1967	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	2	95	theme	granules	277:284	arg1	formula					306:312	a classic formula	296:312	a classic formula for the treatment of respiratory diseases	296:354	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	2	95	theme	granules	277:284	arg1	CHQW					287:290	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	1	96	theme	clinical	151:158	arg1	practice					160:167	clinical practice	151:167	clinical practice	151:167	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	10	97	theme	pathological	1642:1653	arg1	injury					1655:1660	lung tissue pathological injury	1630:1660	lung tissue pathological injury	1630:1660	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	5	98	dep	group	873:877	arg1	8 g/kg					889:894	8 g/kg	889:894	8 g/kg	889:894	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	5	98	dep	group	873:877	arg1	4					883:883	4	883:883	4	883:883	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	5	98	dep	group	873:877	arg1	2					880:880	2	880:880	2	880:880	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	7	99	theme	lavage	1108:1113	arg1	BALF					1122:1125	BALF	1122:1125	BALF	1122:1125	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	99	theme	lavage	1108:1113	arg1	fluid					1115:1119	bronchoalveolar lavage fluid	1092:1119	bronchoalveolar lavage fluid (BALF)	1092:1126	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	2	100	theme	Chaihu	262:267	arg1	formula					306:312	a classic formula	296:312	a classic formula for the treatment of respiratory diseases	296:354	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	2	100	theme	Chaihu	262:267	arg1	CHQW					287:290	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW)	262:291	Chaihu Qingwen granules (CHQW) is a classic formula for the treatment of respiratory diseases.
37315646	8	101	theme	NLR	1320:1322	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	101	theme	NLR	1320:1322	arg1	domain					1337:1342	NLR family pyrin domain	1320:1342	NLR family pyrin domain	1320:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	102	theme	inflammation-related	1169:1188	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	102	theme	inflammation-related	1169:1188	arg1	receptor					1209:1216	toll-like receptor 4	1199:1218	toll-like receptor 4 (TLR4)	1199:1225	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	102	theme	inflammation-related	1169:1188	arg1	B					1305:1305	nuclear-factor-kappa B	1284:1305	nuclear-factor-kappa B (NF-κB)	1284:1313	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	102	theme	inflammation-related	1169:1188	arg1	phospho-IκBα					1261:1272	phospho-IκBα (p-IκBα)	1261:1281	phospho-IκBα (p-IκBα)	1261:1281	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	102	theme	inflammation-related	1169:1188	arg1	alpha					1247:1251	inhibitory kappa B alpha	1228:1251	inhibitory kappa B alpha (IκBα)	1228:1258	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	102	theme	inflammation-related	1169:1188	arg1	domain					1337:1342	NLR family pyrin domain	1320:1342	NLR family pyrin domain	1320:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	1	103	theme	lung	176:179	arg1	injury					181:186	acute lung injury	170:186	acute lung injury	170:186	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	11	104	theme	IκBα	1920:1923	arg1	level					1911:1915	the level	1907:1915	the level of IκBα	1907:1923	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	4	105	theme	study	484:488	arg1	aim					472:474	The aim	468:474	The aim of this study	468:488	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	8	106	theme	toll-like	1199:1207	arg1	TLR4					1221:1224	TLR4	1221:1224	TLR4	1221:1224	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	106	theme	toll-like	1199:1207	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	106	theme	toll-like	1199:1207	arg1	receptor					1209:1216	toll-like receptor 4	1199:1218	toll-like receptor 4 (TLR4)	1199:1225	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	7	107	theme	inflammatory	1068:1079	arg1	factors					1081:1087	inflammatory factors	1068:1087	inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats	1068:1148	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	4	108	dep	was	490:492	arg1	AIM					451:453	AIM	451:453	AIM OF THE STUDY	451:466	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	5	109	theme	CHQW	868:871	arg1	group					873:877	the CHQW group	864:877	the CHQW group (2, 4 and 8 g/kg, respectively)	864:909	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	11	110	theme	proteins	1887:1894	arg1	expression					1850:1859	the expression	1846:1859	the expression of TLR4, p-IκBα and NF-κB proteins	1846:1894	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	12	111	theme	components	2089:2098	arg1	total					2077:2081	a total	2075:2081	a total of 48 components	2075:2098	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	4	112	theme	substance	686:694	arg1	composition					696:706	its substance composition	682:706	its substance composition	682:706	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	1	113	theme	ETHNOPHARMACOLOGICAL	76:95	arg1	RELEVANCE					97:105	ETHNOPHARMACOLOGICAL RELEVANCE	76:105	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice	76:167	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	14	114	theme	activation	2585:2594	arg1	inhibition					2528:2537	the inhibition	2524:2537	the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation	2524:2594	The protective mechanism of CHQW may be related to the inhibition of the TLR4/NF-κB signaling pathway and NLRP3 activation.
37315646	5	115	theme	capsule	846:852	arg1	group					854:858	the Lianhua Qingwen capsule group	826:858	the Lianhua Qingwen capsule group	826:858	MATERIALS AND METHODS Male SD rats were randomly divided into the blank group, the model group, the ibuprofen group, the Lianhua Qingwen capsule group and the CHQW group (2, 4 and 8 g/kg, respectively).
37315646	11	116	theme	NF-κB	1881:1885	arg1	proteins					1887:1894	NF-κB proteins	1881:1894	NF-κB proteins	1881:1894	In addition, CHQW decreased the expression of TLR4, p-IκBα and NF-κB proteins, increased the level of IκBα, regulated the TLR4/NF-κB signaling pathway, and inhibited the activation of NLRP3.
37315646	0	117	theme	component	29:37	arg1	analysis					39:46	component analysis	29:46	component analysis of Chaihu Qingwen granules	29:73	Anti-inflammatory effect and component analysis of Chaihu Qingwen granules.
37315646	1	118	theme	acute	120:124	arg1	condition					138:146	prevalent acute respiratory condition	110:146	prevalent acute respiratory condition	110:146	ETHNOPHARMACOLOGICAL RELEVANCE As prevalent acute respiratory condition in clinical practice, acute lung injury has a quick start and severe symptoms which can harm patients physically.
37315646	6	119	theme	lung	934:937	arg1	ALI					947:949	ALI	947:949	ALI	947:949	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	6	119	theme	lung	934:937	arg1	injury					939:944	LPS-induced acute lung injury	916:944	The LPS-induced acute lung injury (ALI) model in rats	912:964	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	4	120	theme	CHQW	541:544	arg1	effect					531:536	the anti-inflammatory effect	509:536	the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats	509:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	8	121	theme	kappa	1239:1243	arg1	IκBα					1254:1257	IκBα	1254:1257	IκBα	1254:1257	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	121	theme	kappa	1239:1243	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	121	theme	kappa	1239:1243	arg1	alpha					1247:1251	inhibitory kappa B alpha	1228:1251	inhibitory kappa B alpha (IκBα)	1228:1258	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	13	122	theme	lung	2387:2390	arg1	lesions					2399:2405	lung tissue lesions	2387:2405	lung tissue lesions	2387:2405	CONCLUSION The results of this study showed that the pretreatment of CHQW had a strong protective effect on LPS-induced ALI in rats, reducing lung tissue lesions and decreasing inflammatory cytokines released in BALF and serum.
37315646	6	123	theme	LPS-induced	916:926	arg1	ALI					947:949	ALI	947:949	ALI	947:949	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	6	123	theme	LPS-induced	916:926	arg1	injury					939:944	LPS-induced acute lung injury	916:944	The LPS-induced acute lung injury (ALI) model in rats	912:964	The LPS-induced acute lung injury (ALI) model in rats was established after pre-administration.
37315646	7	124	dep	fluid	1115:1119	arg1	rats					1145:1148	rats	1145:1148	rats	1145:1148	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	10	125	theme	cytokines	1728:1736	arg1	release					1704:1710	the release	1700:1710	the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α)	1700:1797	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	4	126	theme	lung	588:591	arg1	ALI					601:603	ALI	601:603	ALI	601:603	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	4	126	theme	lung	588:591	arg1	injury					593:598	acute lung injury	582:598	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats	549:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	0	127	theme	Qingwen	58:64	arg1	granules					66:73	Chaihu Qingwen granules	51:73	Chaihu Qingwen granules	51:73	Anti-inflammatory effect and component analysis of Chaihu Qingwen granules.
37315646	8	128	dep	proteins	1190:1197	arg1	IκBα					1254:1257	IκBα	1254:1257	IκBα	1254:1257	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	proteins					1190:1197	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain	1165:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels	1165:1380	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	receptor					1209:1216	toll-like receptor 4	1199:1218	toll-like receptor 4 (TLR4)	1199:1225	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	B					1305:1305	nuclear-factor-kappa B	1284:1305	nuclear-factor-kappa B (NF-κB)	1284:1313	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	phospho-IκBα					1261:1272	phospho-IκBα (p-IκBα)	1261:1281	phospho-IκBα (p-IκBα)	1261:1281	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	TLR4					1221:1224	TLR4	1221:1224	TLR4	1221:1224	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	NF-κB					1308:1312	NF-κB	1308:1312	NF-κB	1308:1312	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	alpha					1247:1251	inhibitory kappa B alpha	1228:1251	inhibitory kappa B alpha (IκBα)	1228:1258	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	8	128	dep	proteins	1190:1197	arg1	domain					1337:1342	NLR family pyrin domain	1320:1342	NLR family pyrin domain	1320:1342	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	7	129	theme	ALI	1141:1143	arg1	BALF					1122:1125	BALF	1122:1125	BALF	1122:1125	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	129	theme	ALI	1141:1143	arg1	serum					1132:1136	serum	1132:1136	serum of ALI	1132:1143	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	7	129	theme	ALI	1141:1143	arg1	fluid					1115:1119	bronchoalveolar lavage fluid	1092:1119	bronchoalveolar lavage fluid (BALF)	1092:1126	The histopathological changes in the lung and the levels of inflammatory factors in bronchoalveolar lavage fluid (BALF) and serum of ALI rats were observed.
37315646	8	130	theme	western	1399:1405	arg1	analysis					1416:1423	western blotting analysis	1399:1423	western blotting analysis	1399:1423	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
37315646	10	131	theme	tumor	1774:1778	arg1	factor-α					1789:1796	tumor necrosis factor-α	1774:1796	tumor necrosis factor-α	1774:1796	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	10	131	theme	tumor	1774:1778	arg1	cytokines					1728:1736	inflammatory cytokines	1715:1736	inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α)	1715:1797	RESULTS CHQW significantly ameliorated lung tissue pathological injury in LPS-induced ALI rats and decreased the release of inflammatory cytokines (interleukin-1β, interleukin-17 and tumor necrosis factor-α) in BALF and serum.
37315646	12	132	theme	CHQW	2036:2039	arg1	components					2022:2031	The chemical components	2009:2031	The chemical components of CHQW	2009:2039	The chemical components of CHQW were analyzed by LC-Q-TOF-MS, and a total of 48 components were identified by combining information from the literature, mainly flavonoids, organic acids, lignans, iridoids and phenylethanoid glycosides.
37315646	4	133	theme	-induced	573:580	arg1	model					606:610	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model	549:610	lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats	549:618	AIM OF THE STUDY The aim of this study was to investigate the anti-inflammatory effect of CHQW on lipopolysaccharide (LPS)-induced acute lung injury (ALI) model in rats and to explore its potential mechanism, as well as to clarify its substance composition.
37315646	8	134	theme	immunohistochemical	1429:1447	arg1	analysis					1449:1456	immunohistochemical analysis	1429:1456	immunohistochemical analysis	1429:1456	The inflammation-related proteins toll-like receptor 4 (TLR4), inhibitory kappa B alpha (IκBα), phospho-IκBα (p-IκBα), nuclear-factor-kappa B (NF-κB), and NLR family pyrin domain containing 3(NLRP3) expression levels were measured by western blotting analysis and immunohistochemical analysis.
35938741	4	0	theme	Disease	513:519	arg1	activity					521:528	Disease activity	513:528	Disease activity	513:528	Disease activity was examined macroscopically and histologically.
35938741	13	1	theme	RPZ	1694:1696	arg1	group					1698:1702	the RPZ group	1690:1702	the RPZ group	1690:1702	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	2	2	from	influences	303:312	arg1	injury					360:365	NSAID-induced intestinal injury	335:365	NSAID-induced intestinal injury	335:365	Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are clarified.
35938741	2	2	from	influences	303:312	arg1	mechanisms					386:395	the underlying mechanisms	371:395	the underlying mechanisms	371:395	Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are clarified.
35938741	1	3	theme	-induced	252:259	arg1	enteropathy					261:271	nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy	209:271	nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy	209:271	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	0	4	theme	blockers	81:88	arg1	influence					4:12	The influence	0:12	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury	0:136	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	0	5	from	influence	4:12	arg1	injury					131:136	indomethacin-induced small intestinal injury	93:136	indomethacin-induced small intestinal injury	93:136	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	14	6	dep	CONCLUSION	1705:1714	arg1	indicated					1728:1736	indicated	1728:1736	indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different	1728:1958	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	1	7	theme	gastric	175:181	arg1	GAIs					200:203	GAIs	200:203	GAIs	200:203	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	1	7	theme	gastric	175:181	arg1	inhibitors					188:197	gastric acid inhibitors	175:197	gastric acid inhibitors (GAIs)	175:204	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	6	8	theme	16S	676:678	arg1	sequencing					685:694	16S rDNA sequencing	676:694	16S rDNA sequencing	676:694	16S rDNA sequencing was performed to determine the composition of intestinal flora.
35938741	10	9	theme	gut	1254:1256	arg1	microbiota					1258:1267	the gut microbiota	1250:1267	the gut microbiota	1250:1267	After 5-day administration, GAIs significantly altered the composition of the gut microbiota.
35938741	0	10	theme	small	114:118	arg1	injury					131:136	indomethacin-induced small intestinal injury	93:136	indomethacin-induced small intestinal injury	93:136	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	13	11	theme	IPZ	1627:1629	arg1	IPZ					1627:1629	IPZ	1627:1629	IPZ	1627:1629	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	11	theme	IPZ	1627:1629	arg1	VPZ					1636:1638	VPZ	1636:1638	VPZ	1636:1638	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	11	theme	IPZ	1627:1629	arg1	OPZ					1622:1624	OPZ	1622:1624	OPZ	1622:1624	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	11	theme	IPZ	1627:1629	arg1	groups					1612:1617	the groups	1608:1617	the groups of OPZ, IPZ, and VPZ	1608:1638	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	5	12	theme	plasma	642:647	arg1	levels					668:673	plasma lipopolysaccharide levels	642:673	plasma lipopolysaccharide levels	642:673	The permeability of small intestine was evaluated by measuring plasma lipopolysaccharide levels.
35938741	0	13	theme	indomethacin-induced	93:112	arg1	injury					131:136	indomethacin-induced small intestinal injury	93:136	indomethacin-induced small intestinal injury	93:136	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	3	14	dep	METHODS	412:418	arg1	IND					434:436	IND	434:436	IND; 10 mg/kg/day	434:450	METHODS Indomethacin (IND; 10 mg/kg/day) was administered to mice to induce small intestinal injury.
35938741	3	14	dep	METHODS	412:418	arg1	Indomethacin					420:431	Indomethacin	420:431	METHODS Indomethacin (IND; 10 mg/kg/day)	412:451	METHODS Indomethacin (IND; 10 mg/kg/day) was administered to mice to induce small intestinal injury.
35938741	5	15	theme	lipopolysaccharide	649:666	arg1	levels					668:673	plasma lipopolysaccharide levels	642:673	plasma lipopolysaccharide levels	642:673	The permeability of small intestine was evaluated by measuring plasma lipopolysaccharide levels.
35938741	7	16	theme	barrier	911:917	arg1	integrity					886:894	the integrity	882:894	the integrity of the mucosal barrier	882:917	RESULTS Among the four GAIs, ilaprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier.
35938741	11	17	theme	control	1348:1354	arg1	group					1356:1360	the control group	1344:1360	the control group	1344:1360	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
35938741	1	18	from	influence	162:170	arg1	enteropathy					261:271	nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy	209:271	nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy	209:271	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	12	19	theme	IPZ	1440:1442	arg1	treatment					1444:1452	IPZ treatment	1440:1452	IPZ treatment	1440:1452	After IPZ treatment, the community membership was more assembled in the control group than the IND group.
35938741	1	20	theme	acid	183:186	arg1	GAIs					200:203	GAIs	200:203	GAIs	200:203	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	1	20	theme	acid	183:186	arg1	inhibitors					188:197	gastric acid inhibitors	175:197	gastric acid inhibitors (GAIs)	175:204	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	0	21	theme	intestinal	120:129	arg1	injury					131:136	indomethacin-induced small intestinal injury	93:136	indomethacin-induced small intestinal injury	93:136	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	12	22	theme	community	1459:1467	arg1	membership					1469:1478	the community membership	1455:1478	the community membership	1455:1478	After IPZ treatment, the community membership was more assembled in the control group than the IND group.
35938741	10	23	theme	microbiota	1258:1267	arg1	composition					1235:1245	the composition	1231:1245	the composition of the gut microbiota	1231:1267	After 5-day administration, GAIs significantly altered the composition of the gut microbiota.
35938741	5	24	theme	intestine	605:613	arg1	permeability					583:594	The permeability	579:594	The permeability of small intestine	579:613	The permeability of small intestine was evaluated by measuring plasma lipopolysaccharide levels.
35938741	1	25	dep	BACKGROUND	139:148	arg1	influence					162:170	The influence	158:170	The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy	158:271	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	7	26	theme	mucosal	903:909	arg1	barrier					911:917	the mucosal barrier	899:917	the mucosal barrier	899:917	RESULTS Among the four GAIs, ilaprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier.
35938741	1	27	theme	inhibitors	188:197	arg1	influence					162:170	The influence	158:170	The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy	158:271	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	11	28	theme	IPZ	1405:1407	arg1	treatment					1409:1417	IPZ treatment	1405:1417	IPZ treatment	1405:1417	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
35938741	3	29	dep	IND	434:436	arg1	10 mg/kg/day					439:450	10 mg/kg/day	439:450	IND; 10 mg/kg/day	434:450	METHODS Indomethacin (IND; 10 mg/kg/day) was administered to mice to induce small intestinal injury.
35938741	2	30	theme	GAIs	327:330	arg1	influences					303:312	the influences	299:312	the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms	299:395	Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are clarified.
35938741	6	31	theme	flora	753:757	arg1	composition					727:737	the composition	723:737	the composition of intestinal flora	723:757	16S rDNA sequencing was performed to determine the composition of intestinal flora.
35938741	6	32	theme	rDNA	680:683	arg1	sequencing					685:694	16S rDNA sequencing	676:694	16S rDNA sequencing	676:694	16S rDNA sequencing was performed to determine the composition of intestinal flora.
35938741	14	33	theme	different	1753:1761	arg1	influences					1763:1772	different influences	1753:1772	different influences	1753:1772	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	2	34	theme	different	317:325	arg1	GAIs					327:330	different GAIs	317:330	different GAIs	317:330	Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are clarified.
35938741	6	35	theme	intestinal	742:751	arg1	flora					753:757	intestinal flora	742:757	intestinal flora	742:757	16S rDNA sequencing was performed to determine the composition of intestinal flora.
35938741	0	36	theme	different	17:25	arg1	inhibitors					39:48	different proton pump inhibitors	17:48	different proton pump inhibitors	17:48	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	11	37	theme	IND	1274:1276	arg1	group					1278:1282	The IND group	1270:1282	The IND group	1270:1282	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
35938741	9	38	theme	gut	1143:1145	arg1	microbiota					1147:1156	the gut microbiota	1139:1156	the gut microbiota mediated by GAIs	1139:1173	To explore the potential mechanism, we investigated changes in the gut microbiota mediated by GAIs.
35938741	14	39	theme	various	1889:1895	arg1	GAIs					1897:1900	various GAIs	1889:1900	various GAIs	1889:1900	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	7	40	theme	intestinal	849:858	arg1	injury					860:865	IND-induced small intestinal injury	831:865	IND-induced small intestinal injury	831:865	RESULTS Among the four GAIs, ilaprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier.
35938741	13	41	theme	OPZ	1622:1624	arg1	IPZ					1627:1629	IPZ	1627:1629	IPZ	1627:1629	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	41	theme	OPZ	1622:1624	arg1	VPZ					1636:1638	VPZ	1636:1638	VPZ	1636:1638	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	41	theme	OPZ	1622:1624	arg1	OPZ					1622:1624	OPZ	1622:1624	OPZ	1622:1624	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	41	theme	OPZ	1622:1624	arg1	groups					1612:1617	the groups	1608:1617	the groups of OPZ, IPZ, and VPZ	1608:1638	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	11	42	theme	alpha	1314:1318	arg1	diversity					1320:1328	alpha diversity	1314:1328	alpha diversity	1314:1328	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
35938741	0	43	theme	pump	34:37	arg1	inhibitors					39:48	different proton pump inhibitors	17:48	different proton pump inhibitors	17:48	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	11	44	contain	had	1284:1286	arg2	decrease					1302:1309	a significant decrease	1288:1309	a significant decrease in alpha diversity	1288:1328	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
35938741	11	44	contain	had	1284:1286	arg1	group					1278:1282	The IND group	1270:1282	The IND group	1270:1282	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
35938741	14	45	theme	mucosal	1781:1787	arg1	barrier					1789:1795	the mucosal barrier	1777:1795	the mucosal barrier	1777:1795	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	1	46	theme	nonsteroidal	209:220	arg1	drug					240:243	nonsteroidal anti-inflammatory drug	209:243	nonsteroidal anti-inflammatory drug	209:243	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	0	47	theme	proton	27:32	arg1	inhibitors					39:48	different proton pump inhibitors	17:48	different proton pump inhibitors	17:48	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	7	48	theme	IND-induced	831:841	arg1	injury					860:865	IND-induced small intestinal injury	831:865	IND-induced small intestinal injury	831:865	RESULTS Among the four GAIs, ilaprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier.
35938741	14	49	theme	IND-induced	1909:1919	arg1	model					1939:1943	the IND-induced intestinal damage model	1905:1943	the IND-induced intestinal damage model	1905:1943	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	1	50	theme	anti-inflammatory	222:238	arg1	drug					240:243	nonsteroidal anti-inflammatory drug	209:243	nonsteroidal anti-inflammatory drug	209:243	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	7	51	theme	small	843:847	arg1	injury					860:865	IND-induced small intestinal injury	831:865	IND-induced small intestinal injury	831:865	RESULTS Among the four GAIs, ilaprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier.
35938741	11	52	theme	significant	1290:1300	arg1	decrease					1302:1309	a significant decrease	1288:1309	a significant decrease in alpha diversity	1288:1328	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
35938741	12	53	theme	IND	1529:1531	arg1	group					1533:1537	the IND group	1525:1537	the IND group	1525:1537	After IPZ treatment, the community membership was more assembled in the control group than the IND group.
35938741	14	54	theme	microbiota	1849:1858	arg1	composition					1823:1833	the composition	1819:1833	the composition of intestinal microbiota	1819:1858	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	9	55	theme	potential	1091:1099	arg1	mechanism					1101:1109	the potential mechanism	1087:1109	the potential mechanism	1087:1109	To explore the potential mechanism, we investigated changes in the gut microbiota mediated by GAIs.
35938741	0	56	theme	inhibitors	39:48	arg1	influence					4:12	The influence	0:12	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury	0:136	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	8	57	theme	significant	989:999	arg1	differences					1001:1011	significant differences	989:1011	significant differences	989:1011	Omeprazole (OPZ) and vonoprazan (VPZ) ameliorated ulceration without significant differences, while rabeprazole (RPZ) failed to protect against the injury.
35938741	2	58	theme	intestinal	349:358	arg1	injury					360:365	NSAID-induced intestinal injury	335:365	NSAID-induced intestinal injury	335:365	Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are clarified.
35938741	10	59	theme	5-day	1182:1186	arg1	administration					1188:1201	5-day administration	1182:1201	5-day administration	1182:1201	After 5-day administration, GAIs significantly altered the composition of the gut microbiota.
35938741	9	60	from	changes	1128:1134	arg1	microbiota					1147:1156	the gut microbiota	1139:1156	the gut microbiota mediated by GAIs	1139:1173	To explore the potential mechanism, we investigated changes in the gut microbiota mediated by GAIs.
35938741	5	61	theme	small	599:603	arg1	intestine					605:613	small intestine	599:613	small intestine	599:613	The permeability of small intestine was evaluated by measuring plasma lipopolysaccharide levels.
35938741	2	62	theme	NSAID-induced	335:347	arg1	injury					360:365	NSAID-induced intestinal injury	335:365	NSAID-induced intestinal injury	335:365	Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are clarified.
35938741	13	63	theme	VPZ	1636:1638	arg1	IPZ					1627:1629	IPZ	1627:1629	IPZ	1627:1629	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	63	theme	VPZ	1636:1638	arg1	VPZ					1636:1638	VPZ	1636:1638	VPZ	1636:1638	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	63	theme	VPZ	1636:1638	arg1	OPZ					1622:1624	OPZ	1622:1624	OPZ	1622:1624	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	13	63	theme	VPZ	1636:1638	arg1	groups					1612:1617	the groups	1608:1617	the groups of OPZ, IPZ, and VPZ	1608:1638	Further, we found that Lactobacillus was significantly increased in the groups of OPZ, IPZ, and VPZ, while Bacteroides was significantly increased in the RPZ group.
35938741	1	64	dep	-induced	252:259	arg1	drug					240:243	nonsteroidal anti-inflammatory drug	209:243	nonsteroidal anti-inflammatory drug	209:243	BACKGROUND AND AIM The influence of gastric acid inhibitors (GAIs) on nonsteroidal anti-inflammatory drug (NSAID)-induced enteropathy is controversial.
35938741	3	65	theme	intestinal	494:503	arg1	injury					505:510	small intestinal injury	488:510	small intestinal injury	488:510	METHODS Indomethacin (IND; 10 mg/kg/day) was administered to mice to induce small intestinal injury.
35938741	3	66	theme	small	488:492	arg1	injury					505:510	small intestinal injury	488:510	small intestinal injury	488:510	METHODS Indomethacin (IND; 10 mg/kg/day) was administered to mice to induce small intestinal injury.
35938741	0	67	theme	acid	76:79	arg1	blockers					81:88	potassium-competitive acid blockers	54:88	potassium-competitive acid blockers	54:88	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	12	68	theme	control	1506:1512	arg1	group					1514:1518	the control group	1502:1518	the control group than the IND group	1502:1537	After IPZ treatment, the community membership was more assembled in the control group than the IND group.
35938741	14	69	theme	damage	1932:1937	arg1	model					1939:1943	the IND-induced intestinal damage model	1905:1943	the IND-induced intestinal damage model	1905:1943	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	14	70	theme	intestinal	1838:1847	arg1	microbiota					1849:1858	intestinal microbiota	1838:1858	intestinal microbiota	1838:1858	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	14	71	contain	have	1748:1751	arg2	influences					1763:1772	different influences	1753:1772	different influences	1753:1772	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	14	71	contain	have	1748:1751	arg1	GAIs					1743:1746	GAIs	1743:1746	GAIs	1743:1746	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	7	72	dep	RESULTS	760:766	arg1	attenuated					820:829	attenuated	820:829	attenuated IND-induced small intestinal injury	820:865	RESULTS Among the four GAIs, ilaprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier.
35938741	7	72	dep	RESULTS	760:766	arg1	maintained					871:880	maintained	871:880	maintained the integrity of the mucosal barrier	871:917	RESULTS Among the four GAIs, ilaprazole (IPZ) significantly attenuated IND-induced small intestinal injury and maintained the integrity of the mucosal barrier.
35938741	0	73	theme	potassium-competitive	54:74	arg1	blockers					81:88	potassium-competitive acid blockers	54:88	potassium-competitive acid blockers	54:88	The influence of different proton pump inhibitors and potassium-competitive acid blockers on indomethacin-induced small intestinal injury.
35938741	14	74	theme	intestinal	1921:1930	arg1	model					1939:1943	the IND-induced intestinal damage model	1905:1943	the IND-induced intestinal damage model	1905:1943	CONCLUSION Our results indicated that GAIs have different influences on the mucosal barrier, possibly by altering the composition of intestinal microbiota, and the impacts mediated by various GAIs in the IND-induced intestinal damage model seem different.
35938741	2	75	theme	underlying	375:384	arg1	mechanisms					386:395	the underlying mechanisms	371:395	the underlying mechanisms	371:395	Herein, the influences of different GAIs on NSAID-induced intestinal injury and the underlying mechanisms are clarified.
35938741	11	76	from	decrease	1302:1309	arg1	diversity					1320:1328	alpha diversity	1314:1328	alpha diversity	1314:1328	The IND group had a significant decrease in alpha diversity compared with the control group, and this decrease was reversed by OPZ and IPZ treatment, respectively.
36114582	6	0	theme	receptor	939:946	arg1	expression					948:957	upregulated SCFA receptor expression	922:957	upregulated SCFA receptor expression	922:957	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	7	1	theme	intestinal	1140:1149	arg1	composition					1161:1171	intestinal bacterial composition	1140:1171	intestinal bacterial composition	1140:1171	CONCLUSIONS Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal bacterial composition and metabolites.
36114582	7	2	theme	higher	1008:1013	arg1	level					1015:1019	a higher level	1006:1019	a higher level of RhoB	1006:1027	CONCLUSIONS Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal bacterial composition and metabolites.
36114582	5	3	theme	p38	750:752	arg1	MAPK					754:757	p38 MAPK	750:757	p38 MAPK signaling pathway	750:775	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	9	4	dep	Abstract	1293:1300	arg1	Video					1287:1291	Video	1287:1291	Video	1287:1291	Video Abstract.
36114582	4	5	theme	type	484:487	arg1	mice					489:492	wild type mice	479:492	wild type mice	479:492	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	7	6	theme	bacterial	1151:1159	arg1	composition					1161:1171	intestinal bacterial composition	1140:1171	intestinal bacterial composition	1140:1171	CONCLUSIONS Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal bacterial composition and metabolites.
36114582	4	7	theme	DSS-induced	537:547	arg1	colitis					549:555	milder DSS-induced colitis	530:555	milder DSS-induced colitis	530:555	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	4	8	theme	wild	479:482	arg1	mice					489:492	wild type mice	479:492	wild type mice	479:492	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	5	9	theme	goblet	638:643	arg1	differentiation					650:664	goblet cell differentiation	638:664	goblet cell differentiation	638:664	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	6	10	theme	SCFA	826:829	arg1	concentrations					831:844	SCFA concentrations	826:844	SCFA concentrations	826:844	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	4	11	theme	increased	561:569	arg1	numbers					583:589	increased goblet cell numbers	561:589	increased goblet cell numbers	561:589	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	5	12	theme	cell	645:648	arg1	differentiation					650:664	goblet cell differentiation	638:664	goblet cell differentiation	638:664	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	6	13	located	detected	851:858	arg2	concentrations					831:844	SCFA concentrations	826:844	SCFA concentrations	826:844	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	6	13	located	detected	851:858	arg2	bacteria					813:820	increased SCFA-producing bacteria	788:820	increased SCFA-producing bacteria	788:820	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	6	13	located	detected	851:858	arg1	microbiome					874:883	intestinal microbiome	863:883	intestinal microbiome of both RhoB+/- and RhoB-/- mice	863:916	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	6	14	theme	mice	913:916	arg1	microbiome					874:883	intestinal microbiome	863:883	intestinal microbiome of both RhoB+/- and RhoB-/- mice	863:916	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	7	15	theme	cell	1112:1115	arg1	signaling					1117:1125	cell signaling	1112:1125	cell signaling	1112:1125	CONCLUSIONS Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal bacterial composition and metabolites.
36114582	3	16	theme	mice	434:437	arg1	tissues					386:392	colon tissues	380:392	colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis	380:462	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	3	17	theme	colon	380:384	arg1	tissues					386:392	colon tissues	380:392	colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis	380:462	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	6	18	theme	RhoB-/-	905:911	arg1	mice					913:916	both RhoB+/- and RhoB-/- mice	888:916	both RhoB+/- and RhoB-/- mice	888:916	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	6	19	theme	SCFA-producing	798:811	arg1	bacteria					813:820	increased SCFA-producing bacteria	788:820	increased SCFA-producing bacteria	788:820	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	8	20	theme	treatment	1262:1270	arg1	target					1272:1277	a treatment target	1260:1277	a treatment target for UC	1260:1284	These observations suggest that RhoB has potential as a biomarker and a treatment target for UC.
36114582	6	21	theme	increased	788:796	arg1	bacteria					813:820	increased SCFA-producing bacteria	788:820	increased SCFA-producing bacteria	788:820	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	3	22	theme	ulcerative	397:406	arg1	UC					417:418	UC	417:418	UC	417:418	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	3	22	theme	ulcerative	397:406	arg1	colitis					408:414	ulcerative colitis	397:414	ulcerative colitis (UC) patients	397:428	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	6	23	theme	SCFA	934:937	arg1	receptor					939:946	SCFA receptor	934:946	upregulated SCFA receptor expression	922:957	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	5	24	theme	Decreased	614:622	arg1	RhoB					624:627	Decreased RhoB	614:627	Decreased RhoB	614:627	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	3	25	theme	colitis	408:414	arg1	patients					421:428	ulcerative colitis (UC) patients	397:428	ulcerative colitis (UC) patients	397:428	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	4	26	theme	RhoB-/-	507:513	arg1	mice					515:518	RhoB+/- and RhoB-/- mice	495:518	RhoB+/- and RhoB-/- mice	495:518	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	7	27	theme	UC	1078:1079	arg1	development					1081:1091	UC development	1078:1091	UC development	1078:1091	CONCLUSIONS Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal bacterial composition and metabolites.
36114582	4	28	theme	milder	530:535	arg1	colitis					549:555	milder DSS-induced colitis	530:555	milder DSS-induced colitis	530:555	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	3	29	with	patients	421:428	arg1	colitis					456:462	DSS-induced colitis	444:462	DSS-induced colitis	444:462	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	6	30	theme	RhoB+/-	893:899	arg1	mice					913:916	both RhoB+/- and RhoB-/- mice	888:916	both RhoB+/- and RhoB-/- mice	888:916	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	1	31	theme	inflammatory	113:124	arg1	IBD					142:144	IBD	142:144	IBD	142:144	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	1	31	theme	inflammatory	113:124	arg1	diseases					132:139	inflammatory bowel diseases	113:139	inflammatory bowel diseases (IBD)	113:145	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	4	32	theme	IEC	595:597	arg1	proliferation					599:611	IEC proliferation	595:611	IEC proliferation	595:611	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	0	33	theme	cell	40:43	arg1	signaling					45:53	cell signaling	40:53	cell signaling	40:53	RhoB affects colitis through modulating cell signaling and intestinal microbiome.
36114582	1	34	dep	BACKGROUND	82:91	arg1	pathogenesis					97:108	The pathogenesis	93:108	The pathogenesis of inflammatory bowel diseases (IBD)	93:145	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	1	34	dep	BACKGROUND	82:91	arg1	multifactorial					150:163	multifactorial	150:163	multifactorial	150:163	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	1	34	dep	BACKGROUND	82:91	arg1	diagnostic					170:179	diagnostic	170:179	diagnostic	170:179	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	1	34	dep	BACKGROUND	82:91	arg1	remain					214:219	remain	214:219	remain to be developed	214:235	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	5	35	theme	Wnt	713:715	arg1	pathway					727:733	Wnt signaling pathway	713:733	Wnt signaling pathway	713:733	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	5	36	theme	signaling	717:725	arg1	pathway					727:733	Wnt signaling pathway	713:733	Wnt signaling pathway	713:733	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	1	37	theme	treatment	185:193	arg1	strategies					195:204	treatment strategies	185:204	treatment strategies for IBD	185:212	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	3	38	dep	RESULTS	322:328	arg1	found					339:343	found	339:343	found RhoB	339:348	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	3	39	theme	patients	421:428	arg1	tissues					386:392	colon tissues	380:392	colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis	380:462	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	5	40	theme	signaling	759:767	arg1	pathway					769:775	p38 MAPK signaling pathway	750:775	p38 MAPK signaling pathway	750:775	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	1	41	theme	bowel	126:130	arg1	IBD					142:144	IBD	142:144	IBD	142:144	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	1	41	theme	bowel	126:130	arg1	diseases					132:139	inflammatory bowel diseases	113:139	inflammatory bowel diseases (IBD)	113:145	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	3	42	with	mice	434:437	arg1	colitis					456:462	DSS-induced colitis	444:462	DSS-induced colitis	444:462	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	6	43	theme	intestinal	863:872	arg1	microbiome					874:883	intestinal microbiome	863:883	intestinal microbiome of both RhoB+/- and RhoB-/- mice	863:916	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	7	44	theme	RhoB	1024:1027	arg1	level					1015:1019	a higher level	1006:1019	a higher level of RhoB	1006:1027	CONCLUSIONS Taken together, a higher level of RhoB is associated with UC, which also contributes to UC development through modulating cell signaling and altering intestinal bacterial composition and metabolites.
36114582	1	45	theme	diseases	132:139	arg1	pathogenesis					97:108	The pathogenesis	93:108	The pathogenesis of inflammatory bowel diseases (IBD)	93:145	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	1	45	theme	diseases	132:139	arg1	multifactorial					150:163	multifactorial	150:163	multifactorial	150:163	BACKGROUND The pathogenesis of inflammatory bowel diseases (IBD) is multifactorial, and diagnostic and treatment strategies for IBD remain to be developed.
36114582	4	46	theme	RhoB+/-	495:501	arg1	mice					515:518	RhoB+/- and RhoB-/- mice	495:518	RhoB+/- and RhoB-/- mice	495:518	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	5	47	theme	epithelial	670:679	arg1	regeneration					681:692	epithelial regeneration	670:692	epithelial regeneration	670:692	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	2	48	theme	cell	262:265	arg1	functions					267:275	multiple cell functions	253:275	multiple cell functions	253:275	RhoB regulates multiple cell functions; however, its role in colitis is unexplored.
36114582	4	49	theme	cell	578:581	arg1	numbers					583:589	increased goblet cell numbers	561:589	increased goblet cell numbers	561:589	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	2	50	theme	multiple	253:260	arg1	functions					267:275	multiple cell functions	253:275	multiple cell functions	253:275	RhoB regulates multiple cell functions; however, its role in colitis is unexplored.
36114582	3	51	theme	DSS-induced	444:454	arg1	colitis					456:462	DSS-induced colitis	444:462	DSS-induced colitis	444:462	RESULTS Here, we found RhoB was dramatically increased in colon tissues of ulcerative colitis (UC) patients and mice with DSS-induced colitis.
36114582	4	52	theme	goblet	571:576	arg1	numbers					583:589	increased goblet cell numbers	561:589	increased goblet cell numbers	561:589	Compared with wild type mice, RhoB+/- and RhoB-/- mice developed milder DSS-induced colitis and increased goblet cell numbers and IEC proliferation.
36114582	5	53	theme	MAPK	754:757	arg1	pathway					769:775	p38 MAPK signaling pathway	750:775	p38 MAPK signaling pathway	750:775	Decreased RhoB promoted goblet cell differentiation and epithelial regeneration through inhibiting Wnt signaling pathway and activating p38 MAPK signaling pathway.
36114582	6	54	theme	upregulated	922:932	arg1	expression					948:957	upregulated SCFA receptor expression	922:957	upregulated SCFA receptor expression	922:957	Moreover, increased SCFA-producing bacteria and SCFA concentrations were detected in intestinal microbiome of both RhoB+/- and RhoB-/- mice and upregulated SCFA receptor expression was also observed.
36114582	0	55	theme	intestinal	59:68	arg1	microbiome					70:79	intestinal microbiome	59:79	intestinal microbiome	59:79	RhoB affects colitis through modulating cell signaling and intestinal microbiome.
36114582	8	56	contain	has	1227:1229	arg1	RhoB					1222:1225	RhoB	1222:1225	RhoB	1222:1225	These observations suggest that RhoB has potential as a biomarker and a treatment target for UC.
36114582	8	56	contain	has	1227:1229	arg2	target					1272:1277	a treatment target	1260:1277	a treatment target for UC	1260:1284	These observations suggest that RhoB has potential as a biomarker and a treatment target for UC.
36114582	8	56	contain	has	1227:1229	arg2	potential					1231:1239	potential	1231:1239	potential as a biomarker	1231:1254	These observations suggest that RhoB has potential as a biomarker and a treatment target for UC.
36114582	2	57	from	role	291:294	arg1	colitis					299:305	colitis	299:305	colitis	299:305	RhoB regulates multiple cell functions; however, its role in colitis is unexplored.
36521944	2	0	theme	TB	359:360	arg1	assay					376:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	1	1	theme	tuberculosis	263:274	arg1	control					276:282	tuberculosis control	263:282	tuberculosis control	263:282	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	4	2	theme	count	845:849	arg1	patients					805:812	asymptomatic patients	792:812	asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2)	792:907	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	2	theme	count	845:849	arg1	μL					866:867	CD4 count <200 cells per μL	841:867	CD4 count <200 cells per μL	841:867	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	15	3	theme	sensitivity	2426:2436	arg1	tests					2495:2499	potentially promising tests	2473:2499	potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients	2473:2586	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	15	3	theme	sensitivity	2426:2436	arg1	assays					2462:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	6	4	theme	AlereLAM	1080:1087	arg1	assays					1095:1100	FujiLAM and AlereLAM urine assays	1068:1100	assays	1095:1100	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	14	5	dep	%	2361:2361	arg1	%					2376:2376	98%	2374:2376	specificity from 77% (72-81) to 98% (93-99)	2342:2384	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	14	5	dep	%	2361:2361	arg1	to					2371:2372	to	2371:2372	to	2371:2372	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	10	6	theme	FujiLAM	1725:1731	arg1	sensitivity					1733:1743	FujiLAM sensitivity	1725:1743	FujiLAM sensitivity	1725:1743	Using the microbiological reference standard, FujiLAM sensitivity was 60% (95% CI 51-69) and AlereLAM sensitivity was 40% (31-49; p<0·001).
36521944	6	7	theme	sputum	1149:1154	arg1	culture					1156:1162	sputum culture	1149:1162	sputum culture for Mycobacterium tuberculosis	1149:1193	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	6	8	theme	FujiLAM	1068:1074	arg1	assays					1095:1100	FujiLAM and AlereLAM urine assays	1068:1100	assays	1095:1100	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	12	9	with	patients	1975:1982	arg1	counts					1993:1998	CD4 counts	1989:1998	CD4 counts of 200 cells per μL or higher	1989:2028	Among patients with CD4 counts of 200 cells per μL or higher, FujiLAM sensitivity was 47% (34-61) and AlereLAM sensitivity was 24% (14-38; p=0·0116).
36521944	4	10	with	individuals	679:689	arg1	symptoms					713:720	symptoms	713:720	symptoms of tuberculosis irrespective of their CD4 T-cell count	713:775	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	10	with	individuals	679:689	arg1	disease					832:838	advanced HIV disease	819:838	advanced HIV disease	819:838	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	3	11	theme	accuracy	525:532	arg1	study					534:538	a diagnostic accuracy study	512:538	a diagnostic accuracy study	512:538	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	1	12	theme	tests	183:187	arg1	Development					146:156	BACKGROUND Development	135:156	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples	135:243	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	1	12	theme	tests	183:187	arg1	priority					250:257	a priority	248:257	a priority for tuberculosis control	248:282	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	7	13	theme	composite	1355:1363	arg1	standards					1375:1383	microbiological and composite reference standards	1335:1383	microbiological and composite reference standards	1335:1383	Sensitivity and specificity of FujiLAM and AlereLAM were evaluated against microbiological and composite reference standards.
36521944	4	14	theme	HIV	828:830	arg1	disease					832:838	advanced HIV disease	819:838	advanced HIV disease	819:838	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	14	15	theme	from	2354:2357	arg1	%					2361:2361	specificity from 77%	2342:2361	specificity from 77% (72-81) to 98% (93-99)	2342:2384	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	14	15	theme	from	2354:2357	arg1	93-99					2379:2383	93-99	2379:2383	93-99	2379:2383	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	14	15	theme	from	2354:2357	arg1	72-81					2364:2368	72-81	2364:2368	72-81	2364:2368	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	4	16	theme	ambulatory	655:664	arg1	group					778:782	group 1	778:784	group 1	778:784	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	16	theme	ambulatory	655:664	arg1	years					701:705	aged ≥15 years	692:705	aged ≥15 years	692:705	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	16	theme	ambulatory	655:664	arg1	individuals					679:689	ambulatory HIV-positive individuals	655:689	ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1)	655:785	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	3	17	theme	health	566:571	arg1	facilities					573:582	five outpatient public health facilities	543:582	five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa	543:629	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	13	18	theme	standard	2155:2162	arg1	reference					2145:2153	the microbiological reference	2125:2153	the microbiological reference standard	2125:2162	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	15	19	theme	urine-lipoarabinomannan	2438:2460	arg1	tests					2495:2499	potentially promising tests	2473:2499	potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients	2473:2586	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	15	19	theme	urine-lipoarabinomannan	2438:2460	arg1	assays					2462:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	0	20	theme	diagnostic	108:117	arg1	study					128:132	a diagnostic accuracy study	106:132	a diagnostic accuracy study	106:132	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	3	21	theme	outpatient	548:557	arg1	facilities					573:582	five outpatient public health facilities	543:582	five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa	543:629	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	15	22	dep	Next-generation	2402:2416	arg1	higher					2419:2424	higher	2419:2424	higher	2419:2424	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	4	23	theme	clinical	877:884	arg1	stage					886:890	HIV clinical stage 3	873:892	HIV clinical stage 3	873:892	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	15	24	theme	rapid	2512:2516	arg1	diagnosis					2531:2539	rapid tuberculosis diagnosis	2512:2539	rapid tuberculosis diagnosis	2512:2539	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	1	25	theme	non-sputum	226:235	arg1	samples					237:243	non-sputum samples	226:243	non-sputum samples	226:243	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	4	26	theme	cells	856:860	arg1	patients					805:812	asymptomatic patients	792:812	asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2)	792:907	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	26	theme	cells	856:860	arg1	μL					866:867	CD4 count <200 cells per μL	841:867	CD4 count <200 cells per μL	841:867	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	2	27	from	patients	487:494	arg1	samples					461:467	urine samples	455:467	urine samples from HIV-positive patients	455:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	6	28	theme	Ultra	1117:1121	arg1	assay					1123:1127	Xpert MTB/RIF Ultra assay	1103:1127	Xpert MTB/RIF Ultra assay on sputum or urine	1103:1146	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	6	29	theme	Xpert	1103:1107	arg1	assay					1123:1127	Xpert MTB/RIF Ultra assay	1103:1127	Xpert MTB/RIF Ultra assay on sputum or urine	1103:1146	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	10	30	theme	AlereLAM	1772:1779	arg1	sensitivity					1781:1791	AlereLAM sensitivity	1772:1791	AlereLAM sensitivity	1772:1791	Using the microbiological reference standard, FujiLAM sensitivity was 60% (95% CI 51-69) and AlereLAM sensitivity was 40% (31-49; p<0·001).
36521944	11	31	theme	CD4	1839:1841	arg1	counts					1843:1848	CD4 counts	1839:1848	CD4 counts of less than 200 cells per μL	1839:1878	Among patients with CD4 counts of less than 200 cells per μL, FujiLAM sensitivity was 69% (57-79) and AlereLAM sensitivity was 52% (40-64; p=0·0218).
36521944	12	32	theme	CD4	1989:1991	arg1	counts					1993:1998	CD4 counts	1989:1998	CD4 counts of 200 cells per μL or higher	1989:2028	Among patients with CD4 counts of 200 cells per μL or higher, FujiLAM sensitivity was 47% (34-61) and AlereLAM sensitivity was 24% (14-38; p=0·0116).
36521944	6	33	from	assay	1123:1127	arg1	urine					1142:1146	urine	1142:1146	urine	1142:1146	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	6	33	from	assay	1123:1127	arg1	sputum					1132:1137	sputum	1132:1137	sputum	1132:1137	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	0	34	theme	ambulatory	59:68	arg1	patients					70:77	HIV-positive ambulatory patients	46:77	HIV-positive ambulatory patients	46:77	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	5	35	theme	fresh	1024:1028	arg1	sample					1036:1041	a fresh urine sample	1022:1041	a fresh urine sample	1022:1041	All participants underwent clinical examination, chest x-ray, and blood sampling, and were requested to provide a fresh urine sample, and two sputum samples.
36521944	2	36	theme	HIV-positive	474:485	arg1	patients					487:494	HIV-positive patients	474:494	HIV-positive patients	474:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	13	37	dep	CI	2245:2246	arg1	p=0·941					2255:2261	p=0·941	2255:2261	p=0·941	2255:2261	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	4	38	theme	aged	692:695	arg1	years					701:705	aged ≥15 years	692:705	aged ≥15 years	692:705	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	38	theme	aged	692:695	arg1	individuals					679:689	ambulatory HIV-positive individuals	655:689	ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1)	655:785	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	1	39	theme	rapid	161:165	arg1	tests					183:187	rapid biomarker-based tests	161:187	rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples	161:243	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	17	40	theme	Médecins	2714:2721	arg1	Sans					2723:2726	Médecins Sans	2714:2726	Médecins Sans	2714:2726	FUNDING ANRS and Médecins Sans Frontières.
36521944	0	41	theme	African	87:93	arg1	countries					95:103	four African countries	82:103	four African countries	82:103	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	8	42	dep	included	1474:1481	arg1	patients					1502:1509	1031 patients	1497:1509	1031 patients in group 1	1497:1520	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	8	42	dep	included	1474:1481	arg1	patients					1530:1537	544 patients	1526:1537	544 patients in group 2	1526:1548	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	2	43	with	accuracy	320:327	arg1	Alere					407:411	the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM)	387:447	the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients	387:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	11	44	theme	AlereLAM	1921:1928	arg1	sensitivity					1930:1940	AlereLAM sensitivity	1921:1940	AlereLAM sensitivity	1921:1940	Among patients with CD4 counts of less than 200 cells per μL, FujiLAM sensitivity was 69% (57-79) and AlereLAM sensitivity was 52% (40-64; p=0·0218).
36521944	4	45	theme	T-cell	764:769	arg1	count					771:775	their CD4 T-cell count	754:775	their CD4 T-cell count	754:775	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	9	46	theme	patients	1617:1624	arg1	%					1648:1648	3·3%	1645:1648	3·3%	1645:1648	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	46	theme	patients	1617:1624	arg1	%					1606:1606	9·4%	1603:1606	9·4%	1603:1606	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	46	theme	patients	1617:1624	arg1	18					1641:1642	18	1641:1642	18	1641:1642	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	46	theme	patients	1617:1624	arg1	96					1599:1600	96	1599:1600	96	1599:1600	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	46	theme	patients	1617:1624	arg1	patients					1658:1665	542 patients	1654:1665	542 patients in group 2	1654:1676	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	46	theme	patients	1617:1624	arg1	patients					1617:1624	1022 patients	1612:1624	1022 patients in group 1	1612:1635	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	15	47	theme	Next-generation	2402:2416	arg1	tests					2495:2499	potentially promising tests	2473:2499	potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients	2473:2586	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	15	47	theme	Next-generation	2402:2416	arg1	assays					2462:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	8	48	from	patients	1530:1537	arg1	group					1514:1518	group 1	1514:1520	group 1	1514:1520	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	8	48	from	patients	1530:1537	arg1	group					1542:1546	group 2	1542:1548	group 2	1542:1548	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	2	49	theme	assay	376:380	arg1	accuracy					320:327	the diagnostic accuracy	305:327	the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients	305:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	0	50	theme	Novel	0:4	arg1	assay					14:18	Novel FujiLAM assay	0:18	Novel FujiLAM assay	0:18	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	11	51	with	patients	1825:1832	arg1	counts					1843:1848	CD4 counts	1839:1848	CD4 counts of less than 200 cells per μL	1839:1878	Among patients with CD4 counts of less than 200 cells per μL, FujiLAM sensitivity was 69% (57-79) and AlereLAM sensitivity was 52% (40-64; p=0·0218).
36521944	4	52	theme	irrespective	738:749	arg1	symptoms					713:720	symptoms	713:720	symptoms of tuberculosis irrespective of their CD4 T-cell count	713:775	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	2	53	theme	TB-LAM	423:428	arg1	AlereLAM					439:446	AlereLAM	439:446	AlereLAM	439:446	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	2	53	theme	TB-LAM	423:428	arg1	test					433:436	TB-LAM Ag test	423:436	the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients	387:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	9	54	from	patients	1658:1665	arg1	group					1670:1674	group 2	1670:1676	group 2	1670:1676	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	2	55	dep	Alere	407:411	arg1	AlereLAM					439:446	AlereLAM	439:446	AlereLAM	439:446	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	2	55	dep	Alere	407:411	arg1	Determine					413:421	Determine	413:421	Determine	413:421	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	2	55	dep	Alere	407:411	arg1	test					433:436	TB-LAM Ag test	423:436	the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients	387:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	9	56	theme	18	1641:1642	arg1	%					1648:1648	3·3%	1645:1648	3·3%	1645:1648	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	56	theme	18	1641:1642	arg1	%					1606:1606	9·4%	1603:1606	9·4%	1603:1606	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	56	theme	18	1641:1642	arg1	18					1641:1642	18	1641:1642	18	1641:1642	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	56	theme	18	1641:1642	arg1	96					1599:1600	96	1599:1600	96	1599:1600	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	56	theme	18	1641:1642	arg1	patients					1658:1665	542 patients	1654:1665	542 patients in group 2	1654:1676	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	56	theme	18	1641:1642	arg1	patients					1617:1624	1022 patients	1612:1624	1022 patients in group 1	1612:1635	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	15	57	theme	promising	2485:2493	arg1	tests					2495:2499	potentially promising tests	2473:2499	potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients	2473:2586	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	15	57	theme	promising	2485:2493	arg1	assays					2462:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays	2387:2467	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	4	58	theme	asymptomatic	792:803	arg1	patients					805:812	asymptomatic patients	792:812	asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2)	792:907	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	58	theme	asymptomatic	792:803	arg1	μL					866:867	CD4 count <200 cells per μL	841:867	CD4 count <200 cells per μL	841:867	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	10	59	theme	standard	1715:1722	arg1	reference					1705:1713	the microbiological reference	1685:1713	the microbiological reference standard	1685:1722	Using the microbiological reference standard, FujiLAM sensitivity was 60% (95% CI 51-69) and AlereLAM sensitivity was 40% (31-49; p<0·001).
36521944	2	60	theme	SILVAMP	351:357	arg1	assay					376:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	5	61	theme	sputum	1052:1057	arg1	samples					1059:1065	two sputum samples	1048:1065	two sputum samples	1048:1065	All participants underwent clinical examination, chest x-ray, and blood sampling, and were requested to provide a fresh urine sample, and two sputum samples.
36521944	5	62	theme	chest	959:963	arg1	x-ray					965:969	chest x-ray	959:969	chest x-ray	959:969	All participants underwent clinical examination, chest x-ray, and blood sampling, and were requested to provide a fresh urine sample, and two sputum samples.
36521944	8	63	theme	%	1459:1459	arg1	patients					1440:1447	1575 patients	1435:1447	1575 patients (823 [52·3%] women)	1435:1467	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	8	63	theme	%	1459:1459	arg1	women					1462:1466	823 [52·3%] women	1450:1466	823 [52·3%] women	1450:1466	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	2	64	theme	novel	336:340	arg1	assay					376:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	16	65	theme	FujiLAM	2634:2640	arg1	numbers					2646:2652	FujiLAM lot numbers	2634:2652	FujiLAM lot numbers	2634:2652	However, the variability in accuracy between FujiLAM lot numbers needs to be addressed before clinical use.
36521944	6	66	from	culture	1156:1162	arg1	urine					1142:1146	urine	1142:1146	urine	1142:1146	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	6	66	from	culture	1156:1162	arg1	sputum					1132:1137	sputum	1132:1137	sputum	1132:1137	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	12	67	theme	FujiLAM	2031:2037	arg1	sensitivity					2039:2049	FujiLAM sensitivity	2031:2049	FujiLAM sensitivity	2031:2049	Among patients with CD4 counts of 200 cells per μL or higher, FujiLAM sensitivity was 47% (34-61) and AlereLAM sensitivity was 24% (14-38; p=0·0116).
36521944	2	68	theme	FujiLAM	367:373	arg1	assay					376:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	9	69	theme	patients	1658:1665	arg1	18					1641:1642	18	1641:1642	18	1641:1642	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	69	theme	patients	1658:1665	arg1	%					1648:1648	3·3%	1645:1648	3·3%	1645:1648	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	9	69	theme	patients	1658:1665	arg1	patients					1658:1665	542 patients	1654:1665	542 patients in group 2	1654:1676	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	6	70	theme	Mycobacterium	1168:1180	arg1	tuberculosis					1182:1193	Mycobacterium tuberculosis	1168:1193	Mycobacterium tuberculosis	1168:1193	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	2	71	theme	LAM	362:364	arg1	assay					376:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	13	72	theme	FujiLAM	2165:2171	arg1	specificity					2173:2183	FujiLAM specificity	2165:2183	FujiLAM specificity	2165:2183	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	7	73	theme	reference	1365:1373	arg1	standards					1375:1383	microbiological and composite reference standards	1335:1383	microbiological and composite reference standards	1335:1383	Sensitivity and specificity of FujiLAM and AlereLAM were evaluated against microbiological and composite reference standards.
36521944	4	74	theme	CD4	841:843	arg1	patients					805:812	asymptomatic patients	792:812	asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2)	792:907	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	74	theme	CD4	841:843	arg1	μL					866:867	CD4 count <200 cells per μL	841:867	CD4 count <200 cells per μL	841:867	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	7	75	theme	microbiological	1335:1349	arg1	standards					1375:1383	microbiological and composite reference standards	1335:1383	microbiological and composite reference standards	1335:1383	Sensitivity and specificity of FujiLAM and AlereLAM were evaluated against microbiological and composite reference standards.
36521944	15	76	theme	HIV-positive	2566:2577	arg1	patients					2579:2586	HIV-positive patients	2566:2586	HIV-positive patients	2566:2586	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	3	77	theme	diagnostic	514:523	arg1	study					534:538	a diagnostic accuracy study	512:538	a diagnostic accuracy study	512:538	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	1	78	theme	biomarker-based	167:181	arg1	tests					183:187	rapid biomarker-based tests	161:187	rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples	161:243	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	9	79	from	patients	1617:1624	arg1	group					1629:1633	group 1	1629:1635	group 1	1629:1635	Tuberculosis was microbiologically confirmed in 96 (9·4%) of 1022 patients in group 1 and 18 (3·3%) of 542 patients in group 2.
36521944	11	80	theme	cells	1867:1871	arg1	counts					1843:1848	CD4 counts	1839:1848	CD4 counts of less than 200 cells per μL	1839:1878	Among patients with CD4 counts of less than 200 cells per μL, FujiLAM sensitivity was 69% (57-79) and AlereLAM sensitivity was 52% (40-64; p=0·0218).
36521944	4	81	theme	Eligible	632:639	arg1	patients					641:648	Eligible patients	632:648	Eligible patients	632:648	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	13	82	theme	microbiological	2129:2143	arg1	reference					2145:2153	the microbiological reference	2125:2153	the microbiological reference standard	2125:2162	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	3	83	theme	public	559:564	arg1	facilities					573:582	five outpatient public health facilities	543:582	five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa	543:629	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	4	84	theme	advanced	819:826	arg1	disease					832:838	advanced HIV disease	819:838	advanced HIV disease	819:838	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	7	85	theme	AlereLAM	1303:1310	arg1	Sensitivity					1260:1270	Sensitivity	1260:1270	Sensitivity	1260:1270	Sensitivity and specificity of FujiLAM and AlereLAM were evaluated against microbiological and composite reference standards.
36521944	7	85	theme	AlereLAM	1303:1310	arg1	specificity					1276:1286	specificity	1276:1286	specificity	1276:1286	Sensitivity and specificity of FujiLAM and AlereLAM were evaluated against microbiological and composite reference standards.
36521944	0	86	theme	accuracy	119:126	arg1	study					128:132	a diagnostic accuracy study	106:132	a diagnostic accuracy study	106:132	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	0	87	dep	detect	23:28	arg1	study					128:132	a diagnostic accuracy study	106:132	a diagnostic accuracy study	106:132	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	2	88	theme	diagnostic	309:318	arg1	accuracy					320:327	the diagnostic accuracy	305:327	the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients	305:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	14	89	theme	specificity	2342:2352	arg1	%					2361:2361	specificity from 77%	2342:2361	specificity from 77% (72-81) to 98% (93-99)	2342:2384	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	14	89	theme	specificity	2342:2352	arg1	93-99					2379:2383	93-99	2379:2383	93-99	2379:2383	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	14	89	theme	specificity	2342:2352	arg1	72-81					2364:2368	72-81	2364:2368	72-81	2364:2368	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	7	90	theme	FujiLAM	1291:1297	arg1	Sensitivity					1260:1270	Sensitivity	1260:1270	Sensitivity	1260:1270	Sensitivity and specificity of FujiLAM and AlereLAM were evaluated against microbiological and composite reference standards.
36521944	7	90	theme	FujiLAM	1291:1297	arg1	specificity					1276:1286	specificity	1276:1286	specificity	1276:1286	Sensitivity and specificity of FujiLAM and AlereLAM were evaluated against microbiological and composite reference standards.
36521944	13	91	theme	%	2196:2196	arg1	%					2191:2191	87%	2189:2191	87% (95% CI 85-89)	2189:2206	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	13	91	theme	%	2196:2196	arg1	CI					2198:2199	95% CI 85-89	2194:2205	95% CI 85-89	2194:2205	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	16	92	from	variability	2602:2612	arg1	accuracy					2617:2624	accuracy	2617:2624	accuracy	2617:2624	However, the variability in accuracy between FujiLAM lot numbers needs to be addressed before clinical use.
36521944	4	93	theme	HIV	873:875	arg1	stage					886:890	HIV clinical stage 3	873:892	HIV clinical stage 3	873:892	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	10	94	dep	%	1799:1799	arg1	p<0·001					1809:1815	p<0·001	1809:1815	p<0·001	1809:1815	Using the microbiological reference standard, FujiLAM sensitivity was 60% (95% CI 51-69) and AlereLAM sensitivity was 40% (31-49; p<0·001).
36521944	10	95	theme	%	1756:1756	arg1	%					1751:1751	60%	1749:1751	60% (95% CI 51-69)	1749:1766	Using the microbiological reference standard, FujiLAM sensitivity was 60% (95% CI 51-69) and AlereLAM sensitivity was 40% (31-49; p<0·001).
36521944	10	95	theme	%	1756:1756	arg1	CI					1758:1759	95% CI 51-69	1754:1765	95% CI 51-69	1754:1765	Using the microbiological reference standard, FujiLAM sensitivity was 60% (95% CI 51-69) and AlereLAM sensitivity was 40% (31-49; p<0·001).
36521944	6	96	theme	CD4	1200:1202	arg1	count					1204:1208	CD4 count	1200:1208	CD4 count	1200:1208	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	13	97	dep	%	2239:2239	arg1	CI					2245:2246	95 CI 84-88	2242:2252	95 CI 84-88; p=0·941	2242:2261	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	6	98	theme	MTB/RIF	1109:1115	arg1	assay					1123:1127	Xpert MTB/RIF Ultra assay	1103:1127	Xpert MTB/RIF Ultra assay on sputum or urine	1103:1146	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	4	99	theme	≥15	697:699	arg1	years					701:705	aged ≥15 years	692:705	aged ≥15 years	692:705	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	99	theme	≥15	697:699	arg1	individuals					679:689	ambulatory HIV-positive individuals	655:689	ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1)	655:785	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	12	100	theme	cells	2007:2011	arg1	counts					1993:1998	CD4 counts	1989:1998	CD4 counts of 200 cells per μL or higher	1989:2028	Among patients with CD4 counts of 200 cells per μL or higher, FujiLAM sensitivity was 47% (34-61) and AlereLAM sensitivity was 24% (14-38; p=0·0116).
36521944	4	101	theme	per	862:864	arg1	patients					805:812	asymptomatic patients	792:812	asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2)	792:907	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	101	theme	per	862:864	arg1	μL					866:867	CD4 count <200 cells per μL	841:867	CD4 count <200 cells per μL	841:867	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	102	dep	μL	866:867	arg1	group					900:904	group 2	900:906	group 2	900:906	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	102	dep	μL	866:867	arg1	stage					886:890	HIV clinical stage 3	873:892	HIV clinical stage 3	873:892	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	102	dep	μL	866:867	arg1	4					897:897	4	897:897	4	897:897	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	5	103	theme	blood	976:980	arg1	sampling					982:989	blood sampling	976:989	blood sampling	976:989	All participants underwent clinical examination, chest x-ray, and blood sampling, and were requested to provide a fresh urine sample, and two sputum samples.
36521944	1	104	theme	BACKGROUND	135:144	arg1	Development					146:156	BACKGROUND Development	135:156	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples	135:243	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	1	104	theme	BACKGROUND	135:144	arg1	priority					250:257	a priority	248:257	a priority for tuberculosis control	248:282	BACKGROUND Development of rapid biomarker-based tests that can diagnose tuberculosis using non-sputum samples is a priority for tuberculosis control.
36521944	6	105	theme	urine	1089:1093	arg1	assays					1095:1100	FujiLAM and AlereLAM urine assays	1068:1100	assays	1095:1100	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	4	106	theme	HIV-positive	666:677	arg1	group					778:782	group 1	778:784	group 1	778:784	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	106	theme	HIV-positive	666:677	arg1	years					701:705	aged ≥15 years	692:705	aged ≥15 years	692:705	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	106	theme	HIV-positive	666:677	arg1	individuals					679:689	ambulatory HIV-positive individuals	655:689	ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1)	655:785	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	6	107	from	assays	1095:1100	arg1	urine					1142:1146	urine	1142:1146	urine	1142:1146	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	6	107	from	assays	1095:1100	arg1	sputum					1132:1137	sputum	1132:1137	sputum	1132:1137	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	17	108	theme	FUNDING	2697:2703	arg1	ANRS					2705:2708	FUNDING ANRS	2697:2708	FUNDING ANRS	2697:2708	FUNDING ANRS and Médecins Sans Frontières.
36521944	5	109	theme	urine	1030:1034	arg1	sample					1036:1041	a fresh urine sample	1022:1041	a fresh urine sample	1022:1041	All participants underwent clinical examination, chest x-ray, and blood sampling, and were requested to provide a fresh urine sample, and two sputum samples.
36521944	16	110	theme	clinical	2683:2690	arg1	use					2692:2694	clinical use	2683:2694	clinical use	2683:2694	However, the variability in accuracy between FujiLAM lot numbers needs to be addressed before clinical use.
36521944	4	111	with	patients	805:812	arg1	symptoms					713:720	symptoms	713:720	symptoms of tuberculosis irrespective of their CD4 T-cell count	713:775	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	4	111	with	patients	805:812	arg1	disease					832:838	advanced HIV disease	819:838	advanced HIV disease	819:838	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	13	112	theme	AlereLAM	2212:2219	arg1	specificity					2221:2231	AlereLAM specificity	2212:2231	AlereLAM specificity	2212:2231	Using the microbiological reference standard, FujiLAM specificity was 87% (95% CI 85-89) and AlereLAM specificity was 86% (95 CI 84-88; p=0·941).
36521944	15	113	theme	tuberculosis	2518:2529	arg1	diagnosis					2531:2539	rapid tuberculosis diagnosis	2512:2539	rapid tuberculosis diagnosis	2512:2539	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	0	114	from	tuberculosis	30:41	arg1	countries					95:103	four African countries	82:103	four African countries	82:103	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	0	114	from	tuberculosis	30:41	arg1	patients					70:77	HIV-positive ambulatory patients	46:77	HIV-positive ambulatory patients	46:77	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	4	115	theme	CD4	760:762	arg1	count					771:775	their CD4 T-cell count	754:775	their CD4 T-cell count	754:775	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	0	116	theme	FujiLAM	6:12	arg1	assay					14:18	Novel FujiLAM assay	0:18	Novel FujiLAM assay	0:18	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	3	117	dep	METHODS	497:503	arg1	did					508:510	did	508:510	did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa	508:629	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	11	118	theme	FujiLAM	1881:1887	arg1	sensitivity					1889:1899	FujiLAM sensitivity	1881:1899	FujiLAM sensitivity	1881:1899	Among patients with CD4 counts of less than 200 cells per μL, FujiLAM sensitivity was 69% (57-79) and AlereLAM sensitivity was 52% (40-64; p=0·0218).
36521944	12	119	theme	AlereLAM	2071:2078	arg1	sensitivity					2080:2090	AlereLAM sensitivity	2071:2090	AlereLAM sensitivity	2071:2090	Among patients with CD4 counts of 200 cells per μL or higher, FujiLAM sensitivity was 47% (34-61) and AlereLAM sensitivity was 24% (14-38; p=0·0116).
36521944	11	120	dep	%	1907:1907	arg1	57-79					1910:1914	57-79	1910:1914	57-79	1910:1914	Among patients with CD4 counts of less than 200 cells per μL, FujiLAM sensitivity was 69% (57-79) and AlereLAM sensitivity was 52% (40-64; p=0·0218).
36521944	3	121	from	facilities	573:582	arg1	Africa					624:629	Africa	624:629	Africa	624:629	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	3	121	from	facilities	573:582	arg1	Uganda					587:592	Uganda	587:592	Uganda	587:592	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	3	121	from	facilities	573:582	arg1	Mozambique					602:611	Mozambique	602:611	Mozambique	602:611	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	3	121	from	facilities	573:582	arg1	Kenya					595:599	Kenya	595:599	Kenya	595:599	METHODS We did a diagnostic accuracy study at five outpatient public health facilities in Uganda, Kenya, Mozambique, and South Africa.
36521944	6	122	from	count	1204:1208	arg1	urine					1142:1146	urine	1142:1146	urine	1142:1146	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	6	122	from	count	1204:1208	arg1	sputum					1132:1137	sputum	1132:1137	sputum	1132:1137	FujiLAM and AlereLAM urine assays, Xpert MTB/RIF Ultra assay on sputum or urine, sputum culture for Mycobacterium tuberculosis, and CD4 count were systematically carried out for all patients.
36521944	14	123	theme	lot	2295:2297	arg1	number					2299:2304	lot number	2295:2304	lot number	2295:2304	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	2	124	theme	Ag	430:431	arg1	AlereLAM					439:446	AlereLAM	439:446	AlereLAM	439:446	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	2	124	theme	Ag	430:431	arg1	test					433:436	TB-LAM Ag test	423:436	the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients	387:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	8	125	from	patients	1502:1509	arg1	group					1514:1518	group 1	1514:1520	group 1	1514:1520	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	8	125	from	patients	1502:1509	arg1	group					1542:1546	group 2	1542:1548	group 2	1542:1548	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	4	126	theme	tuberculosis	725:736	arg1	symptoms					713:720	symptoms	713:720	symptoms of tuberculosis irrespective of their CD4 T-cell count	713:775	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	5	127	theme	clinical	937:944	arg1	examination					946:956	clinical examination	937:956	clinical examination	937:956	All participants underwent clinical examination, chest x-ray, and blood sampling, and were requested to provide a fresh urine sample, and two sputum samples.
36521944	2	128	theme	urine	455:459	arg1	samples					461:467	urine samples	455:467	urine samples from HIV-positive patients	455:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	8	129	theme	[52·3	1454:1458	arg1	patients					1440:1447	1575 patients	1435:1447	1575 patients (823 [52·3%] women)	1435:1467	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	8	129	theme	[52·3	1454:1458	arg1	women					1462:1466	823 [52·3%] women	1450:1466	823 [52·3%] women	1450:1466	FINDINGS Between Aug 24, 2020 and Sept 21, 2021, 1575 patients (823 [52·3%] women) were included in the study: 1031 patients in group 1 and 544 patients in group 2.
36521944	11	130	dep	%	1948:1948	arg1	p=0·0218					1958:1965	p=0·0218	1958:1965	p=0·0218	1958:1965	Among patients with CD4 counts of less than 200 cells per μL, FujiLAM sensitivity was 69% (57-79) and AlereLAM sensitivity was 52% (40-64; p=0·0218).
36521944	2	131	theme	WHO-recommended	391:405	arg1	Alere					407:411	the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM)	387:447	the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients	387:494	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	0	132	theme	HIV-positive	46:57	arg1	patients					70:77	HIV-positive ambulatory patients	46:77	HIV-positive ambulatory patients	46:77	Novel FujiLAM assay to detect tuberculosis in HIV-positive ambulatory patients in four African countries: a diagnostic accuracy study.
36521944	14	133	theme	FujiLAM	2265:2271	arg1	sensitivity					2273:2283	FujiLAM sensitivity	2265:2283	FujiLAM sensitivity	2265:2283	FujiLAM sensitivity varied by lot number from 48% (34-62) to 76% (57-89) and specificity from 77% (72-81) to 98% (93-99).
36521944	2	134	theme	Fujifilm	342:349	arg1	assay					376:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay	332:380	We aimed to compare the diagnostic accuracy of the novel Fujifilm SILVAMP TB LAM (FujiLAM) assay with the WHO-recommended Alere Determine TB-LAM Ag test (AlereLAM) using urine samples from HIV-positive patients.
36521944	16	135	theme	lot	2642:2644	arg1	numbers					2646:2652	FujiLAM lot numbers	2634:2652	FujiLAM lot numbers	2634:2652	However, the variability in accuracy between FujiLAM lot numbers needs to be addressed before clinical use.
36521944	15	136	theme	care	2557:2560	arg1	point					2548:2552	the point	2544:2552	the point of care for HIV-positive patients	2544:2586	INTERPRETATION Next-generation, higher sensitivity urine-lipoarabinomannan assays are potentially promising tests that allow rapid tuberculosis diagnosis at the point of care for HIV-positive patients.
36521944	4	137	theme	count	771:775	arg1	irrespective					738:749	irrespective	738:749	irrespective	738:749	Eligible patients were ambulatory HIV-positive individuals (aged ≥15 years) with symptoms of tuberculosis irrespective of their CD4 T-cell count (group 1), and asymptomatic patients with advanced HIV disease (CD4 count <200 cells per μL, or HIV clinical stage 3 or 4; group 2).
36521944	10	138	theme	microbiological	1689:1703	arg1	reference					1705:1713	the microbiological reference	1685:1713	the microbiological reference standard	1685:1722	Using the microbiological reference standard, FujiLAM sensitivity was 60% (95% CI 51-69) and AlereLAM sensitivity was 40% (31-49; p<0·001).
36419929	7	0	theme	biochemical	1467:1477	arg1	methods					1479:1485	biochemical methods	1467:1485	biochemical methods such as immunoassays	1467:1506	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	7	0	theme	biochemical	1467:1477	arg1	immunoassays					1495:1506	immunoassays	1495:1506	immunoassays	1495:1506	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	6	1	theme	290.08759	1262:1270	arg1	spectra					1218:1224	the MS/MS spectra	1208:1224	the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O)	1208:1283	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	6	2	theme	oxonium	1183:1189	arg1	ions					1200:1203	its characteristic oxonium fragment ions	1164:1203	its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O)	1164:1283	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	4	3	theme	Neu5Gc	580:585	arg1	study					571:575	the study	567:575	the study of Neu5Gc in humans	567:595	Thus, the study of Neu5Gc in humans has important pathological and clinical relevance.
36419929	3	4	theme	cell	545:548	arg1	therapies					550:558	stem cell therapies	540:558	stem cell therapies	540:558	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36419929	7	5	theme	parallel	1434:1441	arg1	monitoring					1452:1461	multiple and parallel reaction monitoring	1421:1461	multiple and parallel reaction monitoring	1421:1461	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	5	6	gly	N-glycopeptides	813:827	arg2	information					854:864	comprehensive information	840:864	comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures)	840:960	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	6	gly	N-glycopeptides	813:827	arg2	N-glycopeptides					813:827	intact N-glycopeptides	806:827	intact N-glycopeptides	806:827	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	7	7	theme	targeted	1393:1400	arg1	monitoring					1452:1461	multiple and parallel reaction monitoring	1421:1461	multiple and parallel reaction monitoring	1421:1461	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	7	7	theme	targeted	1393:1400	arg1	methods					1479:1485	biochemical methods	1467:1485	biochemical methods such as immunoassays	1467:1506	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	7	7	theme	targeted	1393:1400	arg1	methods					1405:1411	targeted MS methods	1393:1411	targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays	1393:1506	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	8	8	from	discovery	1571:1579	arg1	systems					1663:1669	other biological and clinical systems	1633:1669	other biological and clinical systems	1633:1669	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	0	9	theme	Aberrant	0:7	arg1	acid					30:33	Aberrant N-glycolylneuraminic acid	0:33	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells	0:84	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	7	10	theme	multiple	1421:1428	arg1	monitoring					1452:1461	multiple and parallel reaction monitoring	1421:1461	multiple and parallel reaction monitoring	1421:1461	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	6	11	theme	m/z	1236:1238	arg1	spectra					1218:1224	the MS/MS spectra	1208:1224	the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O)	1208:1283	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	4	12	dep	important	601:609	arg1	pathological					611:622	pathological	611:622	pathological	611:622	Thus, the study of Neu5Gc in humans has important pathological and clinical relevance.
36419929	5	13	theme	peptide	867:873	arg1	backbones					875:883	peptide backbones	867:883	peptide backbones	867:883	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	14	theme	linkage	942:948	arg1	structures					950:959	linkage structures	942:959	linkage structures	942:959	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	15	theme	aberrant	706:713	arg1	Neu5Gc					715:720	aberrant Neu5Gc	706:720	aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs)	706:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	16	theme	N-glycopeptides	813:827	arg1	level					797:801	the molecular level	783:801	the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures)	783:960	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	8	17	from	characterization	1585:1600	arg1	systems					1663:1669	other biological and clinical systems	1633:1669	other biological and clinical systems	1633:1669	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	5	18	theme	≤1	1079:1080	arg1	%					1081:1081	control ≤1%	1071:1081	control ≤1%	1071:1081	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	7	19	theme	Neu5Ac	1347:1352	arg1	data					1354:1357	previously reported Neu5Ac data	1327:1357	previously reported Neu5Ac data	1327:1357	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	5	20	theme	discovery	1050:1058	arg1	FDR					1066:1068	FDR	1066:1068	FDR	1066:1068	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	20	theme	discovery	1050:1058	arg1	rate					1060:1063	a spectrum-level false discovery rate	1027:1063	a spectrum-level false discovery rate (FDR)	1027:1069	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	21	theme	monosaccharide	909:922	arg1	compositions					924:935	N-glycan monosaccharide compositions	900:935	N-glycan monosaccharide compositions	900:935	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	8	22	theme	biological	1639:1648	arg1	systems					1663:1669	other biological and clinical systems	1633:1669	other biological and clinical systems	1633:1669	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	0	23	from	acid	30:33	arg1	cells					80:84	cancer stem cells	68:84	cancer stem cells	68:84	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	0	23	from	acid	30:33	arg1	cells					58:62	breast MCF-7 cancer cells	38:62	breast MCF-7 cancer cells	38:62	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	0	24	theme	stem	75:78	arg1	cells					80:84	cancer stem cells	68:84	cancer stem cells	68:84	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	5	25	theme	MCF-7	732:736	arg1	cells					745:749	breast MCF-7 cancer cells	725:749	breast MCF-7 cancer cells	725:749	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	1	26	located	detected	138:145	arg2	Neu5Gc					114:119	Neu5Gc	114:119	Neu5Gc	114:119	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	1	26	located	detected	138:145	arg2	acid					108:111	N-Glycolylneuraminic acid	87:111	N-Glycolylneuraminic acid (Neu5Gc)	87:120	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	1	26	located	detected	138:145	arg1	humans					150:155	humans	150:155	humans	150:155	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	5	27	theme	target-decoy	973:984	arg1	strategy					1014:1021	a target-decoy theoretical database search strategy	971:1021	a target-decoy theoretical database search strategy	971:1021	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	7	28	theme	important	1305:1313	arg1	addition					1315:1322	an important addition	1302:1322	an important addition to previously reported Neu5Ac data	1302:1357	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	7	28	theme	important	1305:1313	arg1	results					1290:1296	The results	1286:1296	The results	1286:1296	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	8	29	theme	MS-based	1514:1521	arg1	method					1541:1546	This MS-based N-glycoproteomics method	1509:1546	This MS-based N-glycoproteomics method	1509:1546	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	2	30	theme	human	378:382	arg1	cells					391:395	human cancer cells	378:395	human cancer cells	378:395	Neu5Gc has been observed in human cancer cells and tissues.
36419929	5	31	theme	molecular	787:795	arg1	level					797:801	the molecular level	783:801	the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures)	783:960	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	1	32	theme	N-Glycolylneuraminic	87:106	arg1	Neu5Gc					114:119	Neu5Gc	114:119	Neu5Gc	114:119	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	1	32	theme	N-Glycolylneuraminic	87:106	arg1	acid					108:111	N-Glycolylneuraminic acid	87:111	N-Glycolylneuraminic acid (Neu5Gc)	87:120	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	0	33	theme	breast	38:43	arg1	cells					58:62	breast MCF-7 cancer cells	38:62	breast MCF-7 cancer cells	38:62	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	5	34	theme	cancer	755:760	arg1	CSCs					774:777	CSCs	774:777	CSCs	774:777	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	34	theme	cancer	755:760	arg1	cells					767:771	cancer stem cells	755:771	cancer stem cells (CSCs)	755:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	0	35	theme	cancer	51:56	arg1	cells					58:62	breast MCF-7 cancer cells	38:62	breast MCF-7 cancer cells	38:62	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	5	36	theme	false	1044:1048	arg1	FDR					1066:1068	FDR	1066:1068	FDR	1066:1068	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	36	theme	false	1044:1048	arg1	rate					1060:1063	a spectrum-level false discovery rate	1027:1063	a spectrum-level false discovery rate (FDR)	1027:1069	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	6	37	theme	N-glycan	1111:1118	arg1	moieties					1120:1127	N-glycan moieties	1111:1127	N-glycan moieties	1111:1127	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	3	38	theme	healthy	468:474	arg1	obstacles					494:502	obstacles	494:502	obstacles to clinical xenotransplantation and stem cell therapies	494:558	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36419929	3	38	theme	healthy	468:474	arg1	humans					476:481	healthy humans	468:481	healthy humans	468:481	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36419929	1	39	theme	cytidine-5'-monophosphate-N-acetylneuraminic	214:257	arg1	CMP-Neu5Ac					265:274	CMP-Neu5Ac	265:274	CMP-Neu5Ac	265:274	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	1	39	theme	cytidine-5'-monophosphate-N-acetylneuraminic	214:257	arg1	acid					259:262	cytidine-5'-monophosphate-N-acetylneuraminic acid	214:262	cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac)	214:275	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	5	40	theme	database	998:1005	arg1	strategy					1014:1021	a target-decoy theoretical database search strategy	971:1021	a target-decoy theoretical database search strategy	971:1021	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	6	41	theme	Neu5Gc	1101:1106	arg1	existence					1088:1096	The existence	1084:1096	The existence of Neu5Gc on N-glycan moieties	1084:1127	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	8	42	theme	putative	1605:1612	arg1	Neu5Gc					1623:1628	putative aberrant Neu5Gc	1605:1628	putative aberrant Neu5Gc	1605:1628	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	6	43	from	existence	1088:1096	arg1	moieties					1120:1127	N-glycan moieties	1111:1127	N-glycan moieties	1111:1127	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	5	44	from	Neu5Gc	715:720	arg1	cells					745:749	breast MCF-7 cancer cells	725:749	breast MCF-7 cancer cells	725:749	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	44	from	Neu5Gc	715:720	arg1	CSCs					774:777	CSCs	774:777	CSCs	774:777	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	44	from	Neu5Gc	715:720	arg1	cells					767:771	cancer stem cells	755:771	cancer stem cells (CSCs)	755:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	2	45	located	observed	366:373	arg1	tissues					401:407	tissues	401:407	tissues	401:407	Neu5Gc has been observed in human cancer cells and tissues.
36419929	2	45	located	observed	366:373	arg1	cells					391:395	human cancer cells	378:395	human cancer cells	378:395	Neu5Gc has been observed in human cancer cells and tissues.
36419929	2	45	located	observed	366:373	arg2	Neu5Gc					350:355	Neu5Gc	350:355	Neu5Gc	350:355	Neu5Gc has been observed in human cancer cells and tissues.
36419929	7	46	theme	reaction	1443:1450	arg1	monitoring					1452:1461	multiple and parallel reaction monitoring	1421:1461	multiple and parallel reaction monitoring	1421:1461	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	8	47	theme	Neu5Gc	1623:1628	arg1	characterization					1585:1600	characterization	1585:1600	characterization	1585:1600	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	8	47	theme	Neu5Gc	1623:1628	arg1	discovery					1571:1579	discovery	1571:1579	discovery	1571:1579	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	3	48	theme	stem	540:543	arg1	therapies					550:558	stem cell therapies	540:558	stem cell therapies	540:558	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36419929	6	49	theme	characteristic	1168:1181	arg1	ions					1200:1203	its characteristic oxonium fragment ions	1164:1203	its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O)	1164:1283	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	8	50	theme	other	1633:1637	arg1	systems					1663:1669	other biological and clinical systems	1633:1669	other biological and clinical systems	1633:1669	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	5	51	theme	comprehensive	840:852	arg1	information					854:864	comprehensive information	840:864	comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures)	840:960	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	7	52	theme	MS	1402:1403	arg1	monitoring					1452:1461	multiple and parallel reaction monitoring	1421:1461	multiple and parallel reaction monitoring	1421:1461	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	7	52	theme	MS	1402:1403	arg1	methods					1479:1485	biochemical methods	1467:1485	biochemical methods such as immunoassays	1467:1506	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	7	52	theme	MS	1402:1403	arg1	methods					1405:1411	targeted MS methods	1393:1411	targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays	1393:1506	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	1	53	theme	hydroxylase	181:191	arg1	enzyme					193:198	the hydroxylase enzyme	177:198	the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc	177:289	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	5	54	dep	strategy	1014:1021	arg1	%					1081:1081	control ≤1%	1071:1081	control ≤1%	1071:1081	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	55	theme	Neu5Gc	715:720	arg1	characterization					686:701	the N-glycoproteomics characterization	664:701	the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs)	664:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	56	theme	control	1071:1077	arg1	%					1081:1081	control ≤1%	1071:1081	control ≤1%	1071:1081	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	4	57	theme	clinical	628:635	arg1	relevance					637:645	important pathological and clinical relevance	601:645	important pathological and clinical relevance	601:645	Thus, the study of Neu5Gc in humans has important pathological and clinical relevance.
36419929	5	58	theme	N-glycan	900:907	arg1	compositions					924:935	N-glycan monosaccharide compositions	900:935	N-glycan monosaccharide compositions	900:935	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	6	59	theme	MS/MS	1212:1216	arg1	spectra					1218:1224	the MS/MS spectra	1208:1224	the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O)	1208:1283	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	5	60	theme	N-glycoproteomics	668:684	arg1	characterization					686:701	the N-glycoproteomics characterization	664:701	the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs)	664:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	61	from	cells	767:771	arg1	characterization					686:701	the N-glycoproteomics characterization	664:701	the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs)	664:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	4	62	contain	has	597:599	arg2	relevance					637:645	important pathological and clinical relevance	601:645	important pathological and clinical relevance	601:645	Thus, the study of Neu5Gc in humans has important pathological and clinical relevance.
36419929	4	62	contain	has	597:599	arg1	study					571:575	the study	567:575	the study of Neu5Gc in humans	567:595	Thus, the study of Neu5Gc in humans has important pathological and clinical relevance.
36419929	5	63	gly	N-glycosites	886:897	arg2	N-glycosites					886:897	N-glycosites	886:897	N-glycosites	886:897	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	7	64	theme	reported	1338:1345	arg1	data					1354:1357	previously reported Neu5Ac data	1327:1357	previously reported Neu5Ac data	1327:1357	The results are an important addition to previously reported Neu5Ac data and can be further validated with targeted MS methods such as multiple and parallel reaction monitoring and biochemical methods such as immunoassays.
36419929	0	65	theme	cancer	68:73	arg1	cells					80:84	cancer stem cells	68:84	cancer stem cells	68:84	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	5	66	theme	intact	806:811	arg1	information					854:864	comprehensive information	840:864	comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures)	840:960	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	66	theme	intact	806:811	arg1	N-glycopeptides					813:827	intact N-glycopeptides	806:827	intact N-glycopeptides	806:827	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	6	67	theme	fragment	1191:1198	arg1	ions					1200:1203	its characteristic oxonium fragment ions	1164:1203	its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O)	1164:1283	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	6	68	from	ions	1200:1203	arg1	spectra					1218:1224	the MS/MS spectra	1208:1224	the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O)	1208:1283	The existence of Neu5Gc on N-glycan moieties was further confirmed according to its characteristic oxonium fragment ions in the MS/MS spectra of either m/z 308.09816 (Neu5Gc) or 290.08759 (Neu5Gc-H2O).
36419929	5	69	theme	stem	762:765	arg1	CSCs					774:777	CSCs	774:777	CSCs	774:777	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	69	theme	stem	762:765	arg1	cells					767:771	cancer stem cells	755:771	cancer stem cells (CSCs)	755:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	70	theme	breast	725:730	arg1	cells					745:749	breast MCF-7 cancer cells	725:749	breast MCF-7 cancer cells	725:749	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	3	71	located	detected	456:463	arg1	obstacles					494:502	obstacles	494:502	obstacles to clinical xenotransplantation and stem cell therapies	494:558	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36419929	3	71	located	detected	456:463	arg1	humans					476:481	healthy humans	468:481	healthy humans	468:481	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36419929	3	71	located	detected	456:463	arg2	antibodies					420:429	antibodies	420:429	antibodies against Neu5Gc	420:444	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36419929	4	72	from	study	571:575	arg1	humans					590:595	humans	590:595	humans	590:595	Thus, the study of Neu5Gc in humans has important pathological and clinical relevance.
36419929	8	73	dep	discovery	1571:1579	arg1	the					1567:1569	the	1567:1569	the	1567:1569	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	8	74	theme	N-glycoproteomics	1523:1539	arg1	method					1541:1546	This MS-based N-glycoproteomics method	1509:1546	This MS-based N-glycoproteomics method	1509:1546	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	2	75	theme	cancer	384:389	arg1	cells					391:395	human cancer cells	378:395	human cancer cells	378:395	Neu5Gc has been observed in human cancer cells and tissues.
36419929	0	76	theme	N-glycolylneuraminic	9:28	arg1	acid					30:33	Aberrant N-glycolylneuraminic acid	0:33	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells	0:84	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	5	77	from	cells	745:749	arg1	characterization					686:701	the N-glycoproteomics characterization	664:701	the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs)	664:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	0	78	theme	MCF-7	45:49	arg1	cells					58:62	breast MCF-7 cancer cells	38:62	breast MCF-7 cancer cells	38:62	Aberrant N-glycolylneuraminic acid in breast MCF-7 cancer cells and cancer stem cells.
36419929	4	79	theme	important	601:609	arg1	relevance					637:645	important pathological and clinical relevance	601:645	important pathological and clinical relevance	601:645	Thus, the study of Neu5Gc in humans has important pathological and clinical relevance.
36419929	5	80	theme	spectrum-level	1029:1042	arg1	FDR					1066:1068	FDR	1066:1068	FDR	1066:1068	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	80	theme	spectrum-level	1029:1042	arg1	rate					1060:1063	a spectrum-level false discovery rate	1027:1063	a spectrum-level false discovery rate (FDR)	1027:1069	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	81	dep	information	854:864	arg1	N-glycosites					886:897	N-glycosites	886:897	N-glycosites	886:897	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	81	dep	information	854:864	arg1	structures					950:959	linkage structures	942:959	linkage structures	942:959	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	81	dep	information	854:864	arg1	backbones					875:883	peptide backbones	867:883	peptide backbones	867:883	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	81	dep	information	854:864	arg1	compositions					924:935	N-glycan monosaccharide compositions	900:935	N-glycan monosaccharide compositions	900:935	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	8	82	theme	clinical	1654:1661	arg1	systems					1663:1669	other biological and clinical systems	1633:1669	other biological and clinical systems	1633:1669	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	1	83	theme	human	326:330	arg1	body					332:335	the human body	322:335	the human body	322:335	N-Glycolylneuraminic acid (Neu5Gc) is not normally detected in humans because humans lack the hydroxylase enzyme that converts cytidine-5'-monophosphate-N-acetylneuraminic acid (CMP-Neu5Ac) to CMP-Neu5Gc; thus, any Neu5Gc appearing in the human body is aberrant.
36419929	5	84	theme	theoretical	986:996	arg1	strategy					1014:1021	a target-decoy theoretical database search strategy	971:1021	a target-decoy theoretical database search strategy	971:1021	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	85	theme	cancer	738:743	arg1	cells					745:749	breast MCF-7 cancer cells	725:749	breast MCF-7 cancer cells	725:749	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	86	from	characterization	686:701	arg1	cells					745:749	breast MCF-7 cancer cells	725:749	breast MCF-7 cancer cells	725:749	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	86	from	characterization	686:701	arg1	CSCs					774:777	CSCs	774:777	CSCs	774:777	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	86	from	characterization	686:701	arg1	cells					767:771	cancer stem cells	755:771	cancer stem cells (CSCs)	755:778	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	5	87	theme	search	1007:1012	arg1	strategy					1014:1021	a target-decoy theoretical database search strategy	971:1021	a target-decoy theoretical database search strategy	971:1021	Here, we report the N-glycoproteomics characterization of aberrant Neu5Gc in breast MCF-7 cancer cells and cancer stem cells (CSCs) at the molecular level of intact N-glycopeptides, including comprehensive information (peptide backbones, N-glycosites, N-glycan monosaccharide compositions, and linkage structures) based on a target-decoy theoretical database search strategy and a spectrum-level false discovery rate (FDR) control ≤1%.
36419929	8	88	theme	aberrant	1614:1621	arg1	Neu5Gc					1623:1628	putative aberrant Neu5Gc	1605:1628	putative aberrant Neu5Gc	1605:1628	This MS-based N-glycoproteomics method can be extended to the discovery and characterization of putative aberrant Neu5Gc in other biological and clinical systems.
36419929	3	89	theme	clinical	507:514	arg1	xenotransplantation					516:534	clinical xenotransplantation	507:534	clinical xenotransplantation	507:534	Moreover, antibodies against Neu5Gc have been detected in healthy humans, which are obstacles to clinical xenotransplantation and stem cell therapies.
36223177	3	0	dep	comparisons	260:270	arg1	the					213:215	the	213:215	the	213:215	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	3	0	dep	comparisons	260:270	arg1	basis					217:221	basis	217:221	basis	217:221	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	10	1	theme	Sutcliffiella	1620:1632	arg1	consequence					1559:1569	a consequence	1557:1569	a consequence	1557:1569	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	10	1	theme	Sutcliffiella	1620:1632	arg1	species					1599:1605	a novel species	1591:1605	a novel species	1591:1605	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	7	2	theme	nucleotide	793:802	arg1	identity					804:811	The average nucleotide identity	781:811	The average nucleotide identity	781:811	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	6	3	with	hybridization	662:674	arg1	strain					690:695	the type strain	681:695	the type strain of S. zhanjiangensis DSM 23010T	681:727	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	5	4	theme	DNA	625:627	arg1	content					633:639	a DNA G+C content	623:639	a DNA G+C content of 37.3 %	623:649	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	3	5	theme	16S	226:228	arg1	comparisons					260:270	16S rRNA gene sequence similarity comparisons	226:270	16S rRNA gene sequence similarity comparisons	226:270	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	8	6	theme	major	1135:1139	arg1	acids					1147:1151	the major fatty acids	1131:1151	the major fatty acids	1131:1151	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	7	theme	strain	1089:1094	arg1	grouping					1073:1080	the grouping	1069:1080	the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids	1069:1151	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	6	8	theme	23010T	722:727	arg1	strain					690:695	the type strain	681:695	the type strain of S. zhanjiangensis DSM 23010T	681:727	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	3	9	theme	similarity	249:258	arg1	comparisons					260:270	16S rRNA gene sequence similarity comparisons	226:270	16S rRNA gene sequence similarity comparisons	226:270	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	10	10	theme	Sutcliffiella	1665:1677	arg1	sp					1693:1694	the name Sutcliffiella rhizosphaerae sp	1656:1694	the name Sutcliffiella rhizosphaerae sp	1656:1694	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	3	11	theme	gene	235:238	arg1	comparisons					260:270	16S rRNA gene sequence similarity comparisons	226:270	16S rRNA gene sequence similarity comparisons	226:270	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	11	12	theme	CIP	1719:1721	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	12	theme	CIP	1719:1721	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	7	13	theme	Sutcliffiella	979:991	arg1	species					958:964	species	958:964	species of the genus Sutcliffiella	958:991	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	7	14	theme	type	942:945	arg1	strains					947:953	the other type strains	932:953	the other type strains of species of the genus Sutcliffiella	932:991	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	4	15	theme	rRNA	435:438	arg1	%					559:559	<98.4 %	553:559	<98.4 %	553:559	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	4	15	theme	rRNA	435:438	arg1	similarities					454:465	The 16S rRNA gene sequence similarities	427:465	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella	427:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	8	16	theme	menaquinone	1360:1370	arg1	quinone					1348:1354	the only quinone	1339:1354	the only quinone	1339:1354	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	16	theme	menaquinone	1360:1370	arg1	MK-7					1372:1375	menaquinone MK-7	1360:1375	menaquinone MK-7	1360:1375	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	7	17	theme	species	958:964	arg1	strains					947:953	the other type strains	932:953	the other type strains of species of the genus Sutcliffiella	932:991	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	8	18	contain	contained	1233:1241	arg1	profile					1225:1231	the polar lipid profile	1209:1231	the polar lipid profile	1209:1231	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	18	contain	contained	1233:1241	arg2	phosphatidylethanolamine					1313:1336	phosphatidylethanolamine	1313:1336	phosphatidylethanolamine	1313:1336	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	18	contain	contained	1233:1241	arg2	phosphatidylglycerol					1288:1307	phosphatidylglycerol	1288:1307	phosphatidylglycerol	1288:1307	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	18	contain	contained	1233:1241	arg2	components					1253:1262	the major components	1243:1262	the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine	1243:1336	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	18	contain	contained	1233:1241	arg2	diphosphatidylglycerol					1264:1285	diphosphatidylglycerol	1264:1285	diphosphatidylglycerol	1264:1285	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	7	19	theme	other	936:940	arg1	strains					947:953	the other type strains	932:953	the other type strains of species of the genus Sutcliffiella	932:991	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	11	20	dep	nov.	1697:1700	arg1	with					1703:1706	with	1703:1706	with	1703:1706	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	8	21	theme	characteristic	1385:1398	arg1	acid					1408:1411	the characteristic diamino acid	1381:1411	the characteristic diamino acid	1381:1411	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	21	theme	characteristic	1385:1398	arg1	acid					1437:1440	meso-diaminopimelic acid	1417:1440	meso-diaminopimelic acid	1417:1440	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	3	22	dep	showed	351:356	arg1	%					423:423	98.7 %	418:423	98.7 %	418:423	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	7	23	theme	JJ-125T	895:901	arg1	assembly					910:917	the JJ-125T genome assembly	891:917	the JJ-125T genome assembly	891:917	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	9	24	theme	Physiological	1443:1455	arg1	results					1478:1484	Physiological and biochemical test results	1443:1484	Physiological and biochemical test results	1443:1484	Physiological and biochemical test results were also different from those of the most closely related species.
36223177	3	25	theme	Sutcliffiella	333:345	arg1	species					312:318	species	312:318	species of the genus Sutcliffiella	312:345	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	9	26	theme	biochemical	1461:1471	arg1	results					1478:1484	Physiological and biochemical test results	1443:1484	Physiological and biochemical test results	1443:1484	Physiological and biochemical test results were also different from those of the most closely related species.
36223177	4	27	theme	sequence	445:452	arg1	%					559:559	<98.4 %	553:559	<98.4 %	553:559	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	4	27	theme	sequence	445:452	arg1	similarities					454:465	The 16S rRNA gene sequence similarities	427:465	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella	427:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	8	28	theme	polar	1213:1217	arg1	profile					1225:1231	the polar lipid profile	1209:1231	the polar lipid profile	1209:1231	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	7	29	theme	hybridization	862:874	arg1	values					876:881	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	781:881	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly	781:917	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	3	30	theme	highest	362:368	arg1	similarities					370:381	the highest similarities	358:381	the highest similarities	358:381	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	2	31	dep	aerobic	61:67	arg1	endospore-forming					94:110	endospore-forming	94:110	endospore-forming	94:110	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	2	31	dep	aerobic	61:67	arg1	Gram-staining-positive					70:91	Gram-staining-positive	70:91	Gram-staining-positive	70:91	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	8	32	with	grouping	1073:1080	arg1	Sutcliffiella					1111:1123	the genus Sutcliffiella	1101:1123	the genus Sutcliffiella	1101:1123	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	0	33	theme	Sutcliffiella	0:12	arg1	sp					28:29	Sutcliffiella rhizosphaerae sp	0:29	Sutcliffiella rhizosphaerae sp.	0:30	Sutcliffiella rhizosphaerae sp.
36223177	7	34	theme	digital	846:852	arg1	hybridization					862:874	digital DNA-DNA hybridization	846:874	digital DNA-DNA hybridization	846:874	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	10	35	theme	novel	1593:1597	arg1	consequence					1559:1569	a consequence	1557:1569	a consequence	1557:1569	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	10	35	theme	novel	1593:1597	arg1	species					1599:1605	a novel species	1591:1605	a novel species	1591:1605	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	9	36	from	those	1511:1515	arg1	different					1496:1504	different	1496:1504	different	1496:1504	Physiological and biochemical test results were also different from those of the most closely related species.
36223177	4	37	theme	genus	528:532	arg1	Sutcliffiella					534:546	the genus Sutcliffiella	524:546	the genus Sutcliffiella	524:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	2	38	theme	Zea	172:174	arg1	maize					165:169	maize	165:169	maize (Zea mays)	165:180	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	2	38	theme	Zea	172:174	arg1	mays					176:179	Zea mays	172:179	Zea mays	172:179	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	4	39	theme	strains	496:502	arg1	sequences					474:482	the sequences	470:482	the sequences of the type strains of other species of the genus Sutcliffiella	470:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	2	40	theme	maize	165:169	arg1	roots					156:160	roots	156:160	roots of maize (Zea mays)	156:180	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	6	41	theme	S.	700:701	arg1	23010T					722:727	S. zhanjiangensis DSM 23010T	700:727	S. zhanjiangensis DSM 23010T	700:727	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	10	42	theme	genus	1614:1618	arg1	Sutcliffiella					1620:1632	the genus Sutcliffiella	1610:1632	the genus Sutcliffiella	1610:1632	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	4	43	theme	other	507:511	arg1	species					513:519	other species	507:519	other species of the genus Sutcliffiella	507:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	11	44	theme	=	1731:1731	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	44	theme	=	1731:1731	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	6	45	theme	%	765:765	arg1	values					741:746	values	741:746	values of 42.3 and 43.9 % (reciprocal)	741:778	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	9	46	theme	related	1537:1543	arg1	species					1545:1551	the most closely related species	1520:1551	the most closely related species	1520:1551	Physiological and biochemical test results were also different from those of the most closely related species.
36223177	7	47	theme	acid	828:831	arg1	identity					833:840	average amino acid identity	814:840	average amino acid identity	814:840	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	11	48	theme	CCM	1746:1748	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	48	theme	CCM	1746:1748	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	7	49	theme	average	814:820	arg1	identity					833:840	average amino acid identity	814:840	average amino acid identity	814:840	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	8	50	theme	Chemotaxonomic	1035:1048	arg1	features					1050:1057	Chemotaxonomic features	1035:1057	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids	1035:1151	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	2	51	attach	isolated	123:130	arg2	bacterium					112:120	An aerobic, Gram-staining-positive, endospore-forming bacterium	58:120	An aerobic, Gram-staining-positive, endospore-forming bacterium	58:120	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	2	51	attach	isolated	123:130	arg1	rhizosphere					141:151	the rhizosphere	137:151	the rhizosphere of roots of maize (Zea mays)	137:180	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	10	52	theme	name	1660:1663	arg1	sp					1693:1694	the name Sutcliffiella rhizosphaerae sp	1656:1694	the name Sutcliffiella rhizosphaerae sp	1656:1694	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	7	53	theme	identity	804:811	arg1	values					876:881	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	781:881	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly	781:917	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	2	54	theme	roots	156:160	arg1	rhizosphere					141:151	the rhizosphere	137:151	the rhizosphere of roots of maize (Zea mays)	137:180	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	5	55	theme	JJ-125T	585:591	arg1	long					610:613	long	610:613	long	610:613	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	5	55	theme	JJ-125T	585:591	arg1	sequence					573:580	The genome sequence	562:580	The genome sequence of JJ-125T	562:591	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	7	56	theme	average	785:791	arg1	identity					804:811	The average nucleotide identity	781:811	The average nucleotide identity	781:811	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	3	57	theme	rRNA	230:233	arg1	comparisons					260:270	16S rRNA gene sequence similarity comparisons	226:270	16S rRNA gene sequence similarity comparisons	226:270	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	8	58	theme	fatty	1141:1145	arg1	acids					1147:1151	the major fatty acids	1131:1151	the major fatty acids	1131:1151	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	2	59	theme	aerobic	61:67	arg1	bacterium					112:120	An aerobic, Gram-staining-positive, endospore-forming bacterium	58:120	An aerobic, Gram-staining-positive, endospore-forming bacterium	58:120	An aerobic, Gram-staining-positive, endospore-forming bacterium, isolated from the rhizosphere of roots of maize (Zea mays), was taxonomically studied.
36223177	5	60	theme	%	649:649	arg1	content					633:639	a DNA G+C content	623:639	a DNA G+C content of 37.3 %	623:649	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	6	61	theme	DSM	718:720	arg1	23010T					722:727	S. zhanjiangensis DSM 23010T	700:727	S. zhanjiangensis DSM 23010T	700:727	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	10	62	theme	rhizosphaerae	1679:1691	arg1	sp					1693:1694	the name Sutcliffiella rhizosphaerae sp	1656:1694	the name Sutcliffiella rhizosphaerae sp	1656:1694	As a consequence, JJ-125T represents a novel species of the genus Sutcliffiella, for which we propose the name Sutcliffiella rhizosphaerae sp.
36223177	8	63	theme	only	1343:1346	arg1	quinone					1348:1354	the only quinone	1339:1354	the only quinone	1339:1354	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	63	theme	only	1343:1346	arg1	MK-7					1372:1375	menaquinone MK-7	1360:1375	menaquinone MK-7	1360:1375	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	7	64	dep	%	1001:1001	arg1	 and					1009:1012	 and	1009:1012	 and	1009:1012	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	7	65	theme	genus	973:977	arg1	Sutcliffiella					979:991	the genus Sutcliffiella	969:991	the genus Sutcliffiella	969:991	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	8	66	theme	genus	1105:1109	arg1	Sutcliffiella					1111:1123	the genus Sutcliffiella	1101:1123	the genus Sutcliffiella	1101:1123	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	3	67	theme	sequence	240:247	arg1	comparisons					260:270	16S rRNA gene sequence similarity comparisons	226:270	16S rRNA gene sequence similarity comparisons	226:270	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	4	68	theme	gene	440:443	arg1	%					559:559	<98.4 %	553:559	<98.4 %	553:559	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	4	68	theme	gene	440:443	arg1	similarities					454:465	The 16S rRNA gene sequence similarities	427:465	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella	427:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	11	69	theme	111883T	1723:1729	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	69	theme	111883T	1723:1729	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	5	70	theme	G+C	629:631	arg1	content					633:639	a DNA G+C content	623:639	a DNA G+C content of 37.3 %	623:649	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	11	71	theme	=	1717:1717	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	71	theme	=	1717:1717	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	7	72	theme	genome	903:908	arg1	assembly					910:917	the JJ-125T genome assembly	891:917	the JJ-125T genome assembly	891:917	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	8	73	theme	diamino	1400:1406	arg1	acid					1408:1411	the characteristic diamino acid	1381:1411	the characteristic diamino acid	1381:1411	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	73	theme	diamino	1400:1406	arg1	acid					1437:1440	meso-diaminopimelic acid	1417:1440	meso-diaminopimelic acid	1417:1440	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	74	dep	components	1253:1262	arg1	diphosphatidylglycerol					1264:1285	diphosphatidylglycerol	1264:1285	diphosphatidylglycerol	1264:1285	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	74	dep	components	1253:1262	arg1	phosphatidylethanolamine					1313:1336	phosphatidylethanolamine	1313:1336	phosphatidylethanolamine	1313:1336	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	74	dep	components	1253:1262	arg1	components					1253:1262	the major components	1243:1262	the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine	1243:1336	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	74	dep	components	1253:1262	arg1	phosphatidylglycerol					1288:1307	phosphatidylglycerol	1288:1307	phosphatidylglycerol	1288:1307	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	11	75	theme	32156T	1737:1742	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	75	theme	32156T	1737:1742	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	4	76	theme	type	491:494	arg1	strains					496:502	the type strains	487:502	the type strains of other species of the genus Sutcliffiella	487:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	4	77	theme	16S	431:433	arg1	rRNA					435:438	16S rRNA	431:438	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella	427:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	11	78	dep	JJ-125T	1708:1714	arg1	strain					1769:1774	the type strain	1760:1774	the type strain	1760:1774	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	5	79	theme	genome	566:571	arg1	long					610:613	long	610:613	long	610:613	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	5	79	theme	genome	566:571	arg1	sequence					573:580	The genome sequence	562:580	The genome sequence of JJ-125T	562:591	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	6	80	theme	DNA-DNA	654:660	arg1	hybridization					662:674	A DNA-DNA hybridization	652:674	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T	652:727	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	9	81	theme	test	1473:1476	arg1	results					1478:1484	Physiological and biochemical test results	1443:1484	Physiological and biochemical test results	1443:1484	Physiological and biochemical test results were also different from those of the most closely related species.
36223177	3	82	theme	genus	327:331	arg1	Sutcliffiella					333:345	the genus Sutcliffiella	323:345	the genus Sutcliffiella	323:345	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	0	83	theme	rhizosphaerae	14:26	arg1	sp					28:29	Sutcliffiella rhizosphaerae sp	0:29	Sutcliffiella rhizosphaerae sp.	0:30	Sutcliffiella rhizosphaerae sp.
36223177	8	84	dep	acids	1147:1151	arg1	e.g.					1126:1129	e.g.	1126:1129	e.g.	1126:1129	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	85	theme	meso-diaminopimelic	1417:1435	arg1	acid					1408:1411	the characteristic diamino acid	1381:1411	the characteristic diamino acid	1381:1411	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	85	theme	meso-diaminopimelic	1417:1435	arg1	acid					1437:1440	meso-diaminopimelic acid	1417:1440	meso-diaminopimelic acid	1417:1440	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	6	86	dep	%	765:765	arg1	reciprocal					768:777	reciprocal	768:777	reciprocal	768:777	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	8	87	theme	lipid	1219:1223	arg1	profile					1225:1231	the polar lipid profile	1209:1231	the polar lipid profile	1209:1231	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	11	88	theme	type	1764:1767	arg1	strain					1769:1774	the type strain	1760:1774	the type strain	1760:1774	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	7	89	theme	DNA-DNA	854:860	arg1	hybridization					862:874	digital DNA-DNA hybridization	846:874	digital DNA-DNA hybridization	846:874	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	4	90	theme	Sutcliffiella	534:546	arg1	species					513:519	other species	507:519	other species of the genus Sutcliffiella	507:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	8	91	theme	major	1247:1251	arg1	diphosphatidylglycerol					1264:1285	diphosphatidylglycerol	1264:1285	diphosphatidylglycerol	1264:1285	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	91	theme	major	1247:1251	arg1	phosphatidylethanolamine					1313:1336	phosphatidylethanolamine	1313:1336	phosphatidylethanolamine	1313:1336	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	91	theme	major	1247:1251	arg1	components					1253:1262	the major components	1243:1262	the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine	1243:1336	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	8	91	theme	major	1247:1251	arg1	phosphatidylglycerol					1288:1307	phosphatidylglycerol	1288:1307	phosphatidylglycerol	1288:1307	Chemotaxonomic features supported the grouping of the strain with the genus Sutcliffiella, e.g. the major fatty acids included iso-C15 : 0, iso-C17 : 1 ω10c and iso-C17 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, the only quinone was menaquinone MK-7 and the characteristic diamino acid was meso-diaminopimelic acid.
36223177	6	92	theme	zhanjiangensis	703:716	arg1	23010T					722:727	S. zhanjiangensis DSM 23010T	700:727	S. zhanjiangensis DSM 23010T	700:727	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	3	93	theme	Sutcliffiella	388:400	arg1	zhanjiangensis					402:415	Sutcliffiella zhanjiangensis	388:415	Sutcliffiella zhanjiangensis	388:415	On the basis of 16S rRNA gene sequence similarity comparisons, strain JJ-125T clustered together with species of the genus Sutcliffiella and showed the highest similarities with Sutcliffiella zhanjiangensis (98.7 %).
36223177	4	94	theme	species	513:519	arg1	strains					496:502	the type strains	487:502	the type strains of other species of the genus Sutcliffiella	487:546	The 16S rRNA gene sequence similarities to the sequences of the type strains of other species of the genus Sutcliffiella were <98.4 %.
36223177	11	95	theme	=	1744:1744	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	95	theme	=	1744:1744	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	7	96	theme	identity	833:840	arg1	values					876:881	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values	781:881	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly	781:917	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	6	97	theme	type	685:688	arg1	strain					690:695	the type strain	681:695	the type strain of S. zhanjiangensis DSM 23010T	681:727	A DNA-DNA hybridization with the type strain of S. zhanjiangensis DSM 23010T resulted in values of 42.3 and 43.9 % (reciprocal).
36223177	11	98	theme	LMG	1733:1735	arg1	9046T					1750:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	= CIP 111883T = LMG 32156T = CCM 9046T	1717:1754	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	11	98	theme	LMG	1733:1735	arg1	JJ-125T					1708:1714	JJ-125T	1708:1714	JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T)	1708:1755	nov., with JJ-125T (= CIP 111883T = LMG 32156T = CCM 9046T) as the type strain.
36223177	7	99	theme	amino	822:826	arg1	identity					833:840	average amino acid identity	814:840	average amino acid identity	814:840	The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values between the JJ-125T genome assembly and those of the other type strains of species of the genus Sutcliffiella were <75%, <80 % and <21 %, respectively.
36223177	5	100	contain	had	619:621	arg1	long					610:613	long	610:613	long	610:613	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	5	100	contain	had	619:621	arg1	sequence					573:580	The genome sequence	562:580	The genome sequence of JJ-125T	562:591	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36223177	5	100	contain	had	619:621	arg2	content					633:639	a DNA G+C content	623:639	a DNA G+C content of 37.3 %	623:649	The genome sequence of JJ-125T was 4 516 360 bp long and had a DNA G+C content of 37.3 %.
36368358	2	0	theme	extract	534:540	arg1	extraction					501:510	Viscozyme-assisted enzymatic extraction	472:510	Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model	472:642	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	3	1	theme	monosaccharide	805:818	arg1	analysis					820:827	monosaccharide analysis	805:827	monosaccharide analysis	805:827	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	3	2	theme	chemical	780:787	arg1	composition					789:799	chemical composition	780:799	chemical composition	780:799	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	7	3	theme	cell	1458:1461	arg1	death					1463:1467	cell death	1458:1467	cell death	1458:1467	Furthermore, in vivo results showed that SCVF5 can potentially downregulate LPS-induced toxicity, cell death, and NO production in LPS-induced zebrafish model.
36368358	0	4	theme	lipopolysaccharide-induced	85:110	arg1	inflammation					112:123	lipopolysaccharide-induced inflammation	85:123	lipopolysaccharide-induced inflammation in RAW 264.7 macrophages	85:148	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	5	5	theme	RAW	1071:1073	arg1	macrophages					1081:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	0	6	theme	RAW	128:130	arg1	macrophages					138:148	RAW 264.7 macrophages	128:148	RAW 264.7 macrophages	128:148	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	6	7	theme	B	1308:1308	arg1	cascade					1320:1326	the nuclear factor-kappa B signaling cascade	1283:1326	the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells	1283:1357	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	6	8	from	cascade	1320:1326	arg1	cells					1353:1357	LPS-induced RAW 264.7 cells	1331:1357	LPS-induced RAW 264.7 cells	1331:1357	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	1	9	theme	inflammatory	315:326	arg1	mediators					328:336	inflammatory mediators	315:336	inflammatory mediators	315:336	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	1	10	theme	prostaglandin	395:407	arg1	E2					409:410	prostaglandin E2	395:410	prostaglandin E2 (PGE2)	395:417	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	1	10	theme	prostaglandin	395:407	arg1	PGE2					413:416	PGE2	413:416	PGE2	413:416	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	4	11	theme	peak	958:961	arg1	vibrations					937:946	the stretching vibrations	922:946	the stretching vibrations of the SO peak at 1240 cm-1	922:974	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	4	12	theme	infrared	848:855	arg1	spectroscopy					865:876	Fourier-transform infrared (FT-IR) spectroscopy	830:876	Fourier-transform infrared (FT-IR) spectroscopy	830:876	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	1	13	theme	mediators	328:336	arg1	secretion					302:310	secretion	302:310	secretion of inflammatory mediators	302:336	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	4	14	from	vibrations	937:946	arg1	1240 cm-1					966:974	1240 cm-1	966:974	1240 cm-1	966:974	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	4	15	theme	stretching	926:935	arg1	vibrations					937:946	the stretching vibrations	922:946	the stretching vibrations of the SO peak at 1240 cm-1	922:974	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	3	16	dep	sulfate	743:749	arg1	contents					762:769	contents	762:769	contents	762:769	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	3	16	dep	sulfate	743:749	arg1	the					731:733	the	731:733	the	731:733	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	1	17	theme	complex	197:203	arg1	response					221:228	a complex host-protective response	195:228	a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2)	195:417	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	1	17	theme	complex	197:203	arg1	Inflammation					179:190	Inflammation	179:190	Inflammation	179:190	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	2	18	theme	enzymatic	491:499	arg1	extraction					501:510	Viscozyme-assisted enzymatic extraction	472:510	Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model	472:642	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	0	19	theme	Sargassum	43:51	arg1	fucoidan					62:69	Sargassum coreanum fucoidan	43:69	Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling	43:176	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	8	20	theme	cosmetic	1705:1712	arg1	industries					1734:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	4	21	theme	FT-IR	858:862	arg1	spectroscopy					865:876	Fourier-transform infrared (FT-IR) spectroscopy	830:876	Fourier-transform infrared (FT-IR) spectroscopy	830:876	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	7	22	dep	in	1373:1374	arg1	vivo					1376:1379	vivo	1376:1379	vivo	1376:1379	Furthermore, in vivo results showed that SCVF5 can potentially downregulate LPS-induced toxicity, cell death, and NO production in LPS-induced zebrafish model.
36368358	1	23	theme	host-protective	205:219	arg1	response					221:228	a complex host-protective response	195:228	a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2)	195:417	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	1	23	theme	host-protective	205:219	arg1	Inflammation					179:190	Inflammation	179:190	Inflammation	179:190	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	2	24	theme	Viscozyme-assisted	472:489	arg1	extraction					501:510	Viscozyme-assisted enzymatic extraction	472:510	Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model	472:642	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	2	25	theme	coreanum	525:532	arg1	extract					534:540	Sargassum coreanum extract	515:540	Sargassum coreanum extract	515:540	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	6	26	theme	LPS-induced	1331:1341	arg1	cells					1353:1357	LPS-induced RAW 264.7 cells	1331:1357	LPS-induced RAW 264.7 cells	1331:1357	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	8	27	theme	potential	1658:1666	arg1	applications					1668:1679	potential applications	1658:1679	potential applications	1658:1679	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	6	28	with	Treatment	1166:1174	arg1	SCVF5					1181:1185	SCVF5	1181:1185	SCVF5	1181:1185	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	7	29	theme	LPS-induced	1491:1501	arg1	model					1513:1517	LPS-induced zebrafish model	1491:1517	LPS-induced zebrafish model	1491:1517	Furthermore, in vivo results showed that SCVF5 can potentially downregulate LPS-induced toxicity, cell death, and NO production in LPS-induced zebrafish model.
36368358	6	30	theme	pro-inflammatory	1198:1213	arg1	production					1224:1233	pro-inflammatory cytokine production	1198:1233	pro-inflammatory cytokine production	1198:1233	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	6	31	dep	such	1236:1239	arg1	as					1241:1242	as	1241:1242	as	1241:1242	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	5	32	theme	cyclooxygenase-2	1137:1152	arg1	expression					1154:1163	inducible NO synthase and cyclooxygenase-2 expression	1111:1163	inducible NO synthase and cyclooxygenase-2 expression	1111:1163	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	4	33	theme	Fourier-transform	830:846	arg1	spectroscopy					865:876	Fourier-transform infrared (FT-IR) spectroscopy	830:876	Fourier-transform infrared (FT-IR) spectroscopy	830:876	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	5	34	theme	NO	1121:1122	arg1	synthase					1124:1131	inducible NO synthase	1111:1131	inducible NO synthase	1111:1131	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	1	35	theme	harmful	238:244	arg1	stimuli					246:252	harmful stimuli	238:252	harmful stimuli	238:252	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	0	36	theme	therapeutic	21:31	arg1	effect					33:38	therapeutic effect	21:38	therapeutic effect	21:38	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	1	37	theme	nitric	344:349	arg1	NO					358:359	NO	358:359	NO	358:359	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	1	37	theme	nitric	344:349	arg1	oxide					351:355	nitric oxide	344:355	nitric oxide (NO)	344:360	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	2	38	theme	RAW	602:604	arg1	macrophages					612:622	RAW 264.7 macrophages	602:622	RAW 264.7 macrophages	602:622	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	0	39	from	inflammation	112:123	arg1	macrophages					138:148	RAW 264.7 macrophages	128:148	RAW 264.7 macrophages	128:148	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	7	40	theme	zebrafish	1503:1511	arg1	model					1513:1517	LPS-induced zebrafish model	1491:1517	LPS-induced zebrafish model	1491:1517	Furthermore, in vivo results showed that SCVF5 can potentially downregulate LPS-induced toxicity, cell death, and NO production in LPS-induced zebrafish model.
36368358	2	41	theme	Sargassum	515:523	arg1	extract					534:540	Sargassum coreanum extract	515:540	Sargassum coreanum extract	515:540	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	8	42	theme	food	1699:1702	arg1	industries					1734:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	8	43	theme	remarkable	1586:1595	arg1	activity					1615:1622	remarkable anti-inflammatory activity	1586:1622	remarkable anti-inflammatory activity	1586:1622	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	4	44	theme	sulfate	904:910	arg1	esters					912:917	sulfate esters	904:917	sulfate esters	904:917	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	8	45	contain	has	1582:1584	arg2	activity					1615:1622	remarkable anti-inflammatory activity	1586:1622	remarkable anti-inflammatory activity	1586:1622	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	8	45	contain	has	1582:1584	arg1	fucoidan					1573:1580	S. coreanum fucoidan	1561:1580	S. coreanum fucoidan	1561:1580	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	6	46	theme	RAW	1343:1345	arg1	cells					1353:1357	LPS-induced RAW 264.7 cells	1331:1357	LPS-induced RAW 264.7 cells	1331:1357	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	6	47	theme	cytokine	1215:1222	arg1	production					1224:1233	pro-inflammatory cytokine production	1198:1233	pro-inflammatory cytokine production	1198:1233	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	8	48	theme	functional	1688:1697	arg1	industries					1734:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	0	49	theme	NF-κB	162:166	arg1	signaling					168:176	NF-κB signaling	162:176	NF-κB signaling	162:176	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	3	50	theme	exchange	693:700	arg1	chromatography					702:715	ion exchange chromatography	689:715	ion exchange chromatography	689:715	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	3	51	theme	fucoidan	655:662	arg1	fractions					664:672	the fucoidan fractions	651:672	the fucoidan fractions isolated using ion exchange chromatography	651:715	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	7	52	theme	NO	1474:1475	arg1	production					1477:1486	NO production	1474:1486	NO production	1474:1486	Furthermore, in vivo results showed that SCVF5 can potentially downregulate LPS-induced toxicity, cell death, and NO production in LPS-induced zebrafish model.
36368358	1	53	theme	macrophage	264:273	arg1	activation					275:284	macrophage activation	264:284	macrophage activation that results in secretion of inflammatory mediators	264:336	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	0	54	theme	fucoidan	62:69	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	0	54	theme	fucoidan	62:69	arg1	effect					33:38	therapeutic effect	21:38	therapeutic effect	21:38	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	2	55	theme	-stimulated	574:584	arg1	inflammation					586:597	lipopolysaccharide (LPS)-stimulated inflammation	550:597	lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model	550:642	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	8	56	theme	S.	1561:1562	arg1	fucoidan					1573:1580	S. coreanum fucoidan	1561:1580	S. coreanum fucoidan	1561:1580	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	5	57	theme	LPS-stimulated	1056:1069	arg1	macrophages					1081:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	5	58	theme	anti-inflammatory	990:1006	arg1	effects					1008:1014	anti-inflammatory effects	990:1014	anti-inflammatory effects	990:1014	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	0	59	theme	coreanum	53:60	arg1	fucoidan					62:69	Sargassum coreanum fucoidan	43:69	Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling	43:176	Characterization and therapeutic effect of Sargassum coreanum fucoidan that inhibits lipopolysaccharide-induced inflammation in RAW 264.7 macrophages by blocking NF-κB signaling.
36368358	2	60	theme	zebrafish	628:636	arg1	model					638:642	zebrafish model	628:642	zebrafish model	628:642	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	3	61	theme	highest	735:741	arg1	sulfate					743:749	sulfate	743:749	sulfate	743:749	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	4	62	theme	esters	912:917	arg1	presence					892:899	the presence	888:899	the presence of sulfate esters	888:917	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	8	63	theme	coreanum	1564:1571	arg1	fucoidan					1573:1580	S. coreanum fucoidan	1561:1580	S. coreanum fucoidan	1561:1580	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	6	64	theme	nuclear	1287:1293	arg1	B					1308:1308	the nuclear factor-kappa B	1283:1308	the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells	1283:1357	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	7	65	theme	LPS-induced	1436:1446	arg1	toxicity					1448:1455	LPS-induced toxicity	1436:1455	LPS-induced toxicity	1436:1455	Furthermore, in vivo results showed that SCVF5 can potentially downregulate LPS-induced toxicity, cell death, and NO production in LPS-induced zebrafish model.
36368358	6	66	theme	signaling	1310:1318	arg1	cascade					1320:1326	the nuclear factor-kappa B signaling cascade	1283:1326	the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells	1283:1357	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	5	67	theme	synthase	1124:1131	arg1	expression					1154:1163	inducible NO synthase and cyclooxygenase-2 expression	1111:1163	inducible NO synthase and cyclooxygenase-2 expression	1111:1163	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	5	68	theme	PGE2	1037:1040	arg1	generation					1042:1051	PGE2 generation	1037:1051	PGE2 generation	1037:1051	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	8	69	theme	anti-inflammatory	1597:1613	arg1	activity					1615:1622	remarkable anti-inflammatory activity	1586:1622	remarkable anti-inflammatory activity	1586:1622	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	3	70	theme	ion	689:691	arg1	chromatography					702:715	ion exchange chromatography	689:715	ion exchange chromatography	689:715	Among the fucoidan fractions isolated using ion exchange chromatography, SCVF5 showed the highest sulfate and fucose contents based on chemical composition and monosaccharide analysis.
36368358	7	71	theme	in	1373:1374	arg1	results					1381:1387	in vivo results	1373:1387	in vivo results	1373:1387	Furthermore, in vivo results showed that SCVF5 can potentially downregulate LPS-induced toxicity, cell death, and NO production in LPS-induced zebrafish model.
36368358	5	72	from	NO	1030:1031	arg1	macrophages					1081:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	2	73	from	inflammation	586:597	arg1	macrophages					612:622	RAW 264.7 macrophages	602:622	RAW 264.7 macrophages	602:622	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	2	73	from	inflammation	586:597	arg1	model					638:642	zebrafish model	628:642	zebrafish model	628:642	In this study, we evaluated fucoidan isolated using Viscozyme-assisted enzymatic extraction of Sargassum coreanum extract against lipopolysaccharide (LPS)-stimulated inflammation in RAW 264.7 macrophages and zebrafish model.
36368358	4	74	from	1240 cm-1	966:974	arg1	vibrations					937:946	the stretching vibrations	922:946	the stretching vibrations of the SO peak at 1240 cm-1	922:974	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	4	74	from	1240 cm-1	966:974	arg1	peak					958:961	the SO peak	951:961	the SO peak at 1240 cm-1	951:974	Fourier-transform infrared (FT-IR) spectroscopy confirmed the presence of sulfate esters by the stretching vibrations of the SO peak at 1240 cm-1.
36368358	6	75	theme	factor-kappa	1295:1306	arg1	B					1308:1308	the nuclear factor-kappa B	1283:1308	the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells	1283:1357	Treatment with SCVF5 suppressed pro-inflammatory cytokine production, such as TNF-α, (IL)-1β, and IL-6 by modulating the nuclear factor-kappa B signaling cascade in LPS-induced RAW 264.7 cells.
36368358	5	76	from	generation	1042:1051	arg1	macrophages					1081:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	LPS-stimulated RAW 264.7 macrophages	1056:1091	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	8	77	theme	pharmaceutical	1719:1732	arg1	industries					1734:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	the functional food, cosmetic, and pharmaceutical industries	1684:1743	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	1	78	theme	pro-inflammatory	363:378	arg1	cytokines					380:388	pro-inflammatory cytokines	363:388	pro-inflammatory cytokines	363:388	Inflammation is a complex host-protective response against harmful stimuli involving macrophage activation that results in secretion of inflammatory mediators, like nitric oxide (NO), pro-inflammatory cytokines, and prostaglandin E2 (PGE2).
36368358	5	79	theme	inducible	1111:1119	arg1	synthase					1124:1131	inducible NO synthase	1111:1131	inducible NO synthase	1111:1131	SCVF5 showed anti-inflammatory effects by inhibiting NO and PGE2 generation in LPS-stimulated RAW 264.7 macrophages by downregulating inducible NO synthase and cyclooxygenase-2 expression.
36368358	8	80	contain	have	1653:1656	arg1	fucoidan					1573:1580	S. coreanum fucoidan	1561:1580	S. coreanum fucoidan	1561:1580	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36368358	8	80	contain	have	1653:1656	arg2	applications					1668:1679	potential applications	1658:1679	potential applications	1658:1679	Collectively, these results suggest that S. coreanum fucoidan has remarkable anti-inflammatory activity in vitro and in vivo and may have potential applications in the functional food, cosmetic, and pharmaceutical industries.
36053746	3	0	theme	IC50	750:753	arg1	nM					760:761	IC50: 3.3 nM	750:761	IC50: 3.3 nM	750:761	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	0	theme	IC50	750:753	arg1	genes					743:747	interferon-stimulated genes	721:747	interferon-stimulated genes (IC50: 3.3 nM)	721:762	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	4	1	dep	-induced	1079:1086	arg1	acid					1068:1071	2,4,6-trinitrobenzenesulfonic acid	1038:1071	2,4,6-trinitrobenzenesulfonic acid	1038:1071	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	4	1	dep	-induced	1079:1086	arg1	DSS					1028:1030	dextran sulfate sodium (DSS)-	1004:1032	dextran sulfate sodium (DSS)-	1004:1032	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	4	2	theme	sulfate	1012:1018	arg1	DSS					1028:1030	dextran sulfate sodium (DSS)-	1004:1032	dextran sulfate sodium (DSS)-	1004:1032	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	1	3	theme	intestinal	261:270	arg1	microbiota					272:281	intestinal microbiota	261:281	intestinal microbiota	261:281	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	0	4	theme	Bowel	77:81	arg1	IBD					92:94	IBD	92:94	IBD	92:94	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	0	4	theme	Bowel	77:81	arg1	Disease					83:89	Inflammatory Bowel Disease	64:89	Inflammatory Bowel Disease (IBD)	64:95	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	3	5	theme	interferon-stimulated	721:741	arg1	nM					760:761	IC50: 3.3 nM	750:761	IC50: 3.3 nM	750:761	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	5	theme	interferon-stimulated	721:741	arg1	genes					743:747	interferon-stimulated genes	721:747	interferon-stimulated genes (IC50: 3.3 nM)	721:762	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	6	theme	potent	684:689	arg1	activities					702:711	potent inhibitory activities	684:711	potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM)	684:798	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	7	theme	proinflammatory	837:851	arg1	TNF-α					914:918	TNF-α	914:918	TNF-α	914:918	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	7	theme	proinflammatory	837:851	arg1	factors					853:859	various proinflammatory factors	829:859	various proinflammatory factors	829:859	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	7	theme	proinflammatory	837:851	arg1	IL-12					921:925	IL-12	921:925	IL-12	921:925	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	7	theme	proinflammatory	837:851	arg1	IL-8					901:904	IL-8	901:904	IL-8	901:904	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	7	theme	proinflammatory	837:851	arg1	IL-1β					907:911	IL-1β	907:911	IL-1β	907:911	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	7	theme	proinflammatory	837:851	arg1	IFN-γ					932:936	IFN-γ	932:936	IFN-γ	932:936	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	7	theme	proinflammatory	837:851	arg1	IL-6					895:898	IL-6	895:898	IL-6	895:898	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	2	8	theme	derivatives	526:536	arg1	series					492:497	a series	490:497	a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB	490:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	3	9	from	level	878:882	arg1	TNF-α					914:918	TNF-α	914:918	TNF-α	914:918	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	9	from	level	878:882	arg1	factors					853:859	various proinflammatory factors	829:859	various proinflammatory factors	829:859	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	9	from	level	878:882	arg1	IL-12					921:925	IL-12	921:925	IL-12	921:925	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	9	from	level	878:882	arg1	IL-8					901:904	IL-8	901:904	IL-8	901:904	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	9	from	level	878:882	arg1	release					818:824	the release	814:824	the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ	814:936	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	9	from	level	878:882	arg1	IL-1β					907:911	IL-1β	907:911	IL-1β	907:911	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	9	from	level	878:882	arg1	IFN-γ					932:936	IFN-γ	932:936	IFN-γ	932:936	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	9	from	level	878:882	arg1	IL-6					895:898	IL-6	895:898	IL-6	895:898	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	5	10	theme	NF-κB	1352:1356	arg1	signals					1358:1364	JAK/STAT and NF-κB signals	1339:1364	JAK/STAT and NF-κB signals	1339:1364	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	2	11	theme	quinoline	516:524	arg1	derivatives					526:536	imidazo[4,5-c]quinoline derivatives	502:536	imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB	502:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	1	12	theme	comprehensive	350:362	arg1	regulation					364:373	the comprehensive regulation	346:373	the comprehensive regulation of these important pathogenic factors	346:411	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	2	13	theme	signaling	609:617	arg1	JAK/STAT					628:635	JAK/STAT	628:635	JAK/STAT	628:635	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	13	theme	signaling	609:617	arg1	pathways					619:626	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	13	theme	signaling	609:617	arg1	NF-κB					641:645	NF-κB	641:645	NF-κB	641:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	4	14	theme	acute	1088:1092	arg1	models					1104:1109	both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models	999:1109	both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models	999:1109	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	4	15	theme	strong	962:967	arg1	activity					987:994	a strong anti-inflammatory activity	960:994	a strong anti-inflammatory activity	960:994	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	4	16	theme	2,4,6-trinitrobenzenesulfonic	1038:1066	arg1	acid					1068:1071	2,4,6-trinitrobenzenesulfonic acid	1038:1071	2,4,6-trinitrobenzenesulfonic acid	1038:1071	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	4	17	theme	-induced	1079:1086	arg1	models					1104:1109	both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models	999:1109	both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models	999:1109	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	2	18	theme	current	461:467	arg1	study					469:473	the current study	457:473	the current study	457:473	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	19	dep	pathways	619:626	arg1	JAK/STAT					628:635	JAK/STAT	628:635	JAK/STAT	628:635	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	19	dep	pathways	619:626	arg1	pathways					619:626	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	19	dep	pathways	619:626	arg1	NF-κB					641:645	NF-κB	641:645	NF-κB	641:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	3	20	from	release	818:824	arg1	level					878:882	the nanomolar level	864:882	the nanomolar level	864:882	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	5	21	theme	simultaneous	1311:1322	arg1	suppression					1324:1334	the simultaneous suppression	1307:1334	the simultaneous suppression of JAK/STAT and NF-κB signals	1307:1364	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	3	22	theme	NF-κB	768:772	arg1	pathways					774:781	NF-κB pathways	768:781	NF-κB pathways (IC50: 150.7 nM)	768:798	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	22	theme	NF-κB	768:772	arg1	nM					796:797	IC50: 150.7 nM	784:797	IC50: 150.7 nM	784:797	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	5	23	theme	suppression	1324:1334	arg1	potential					1294:1302	the great anti-inflammatory potential	1266:1302	the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals	1266:1364	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	4	24	theme	enteritis	1094:1102	arg1	models					1104:1109	both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models	999:1109	both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models	999:1109	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	3	25	theme	inhibitory	691:700	arg1	activities					702:711	potent inhibitory activities	684:711	potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM)	684:798	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	0	26	theme	Imidazo[4,5-c	19:31	arg1	Derivatives					43:53	Novel Imidazo[4,5-c]quinoline Derivatives	13:53	Novel Imidazo[4,5-c]quinoline Derivatives	13:53	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	0	27	theme	Multiple	111:118	arg1	Pathways					146:153	Multiple Proinflammatory Signaling Pathways	111:153	Multiple Proinflammatory Signaling Pathways	111:153	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	5	28	theme	great	1270:1274	arg1	potential					1294:1302	the great anti-inflammatory potential	1266:1302	the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals	1266:1364	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	1	29	theme	complex	198:204	arg1	pathogenesis					206:217	a complex pathogenesis	196:217	a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota	196:281	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	0	30	theme	Novel	13:17	arg1	Derivatives					43:53	Novel Imidazo[4,5-c]quinoline Derivatives	13:53	Novel Imidazo[4,5-c]quinoline Derivatives	13:53	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	1	31	theme	inflammatory	301:312	arg1	IBD					329:331	IBD	329:331	IBD	329:331	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	1	31	theme	inflammatory	301:312	arg1	disease					320:326	inflammatory bowel disease	301:326	inflammatory bowel disease (IBD)	301:332	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	1	32	theme	important	384:392	arg1	factors					405:411	these important pathogenic factors	378:411	these important pathogenic factors	378:411	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	5	33	theme	anti-inflammatory	1276:1292	arg1	potential					1294:1302	the great anti-inflammatory potential	1266:1302	the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals	1266:1364	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	0	34	theme	quinoline	33:41	arg1	Derivatives					43:53	Novel Imidazo[4,5-c]quinoline Derivatives	13:53	Novel Imidazo[4,5-c]quinoline Derivatives	13:53	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	1	35	theme	bowel	314:318	arg1	IBD					329:331	IBD	329:331	IBD	329:331	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	1	35	theme	bowel	314:318	arg1	disease					320:326	inflammatory bowel disease	301:326	inflammatory bowel disease (IBD)	301:332	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	0	36	theme	Signaling	136:144	arg1	Pathways					146:153	Multiple Proinflammatory Signaling Pathways	111:153	Multiple Proinflammatory Signaling Pathways	111:153	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	1	37	theme	disease	320:326	arg1	treatment					288:296	the treatment	284:296	the treatment of inflammatory bowel disease (IBD)	284:332	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	0	38	theme	Proinflammatory	120:134	arg1	Pathways					146:153	Multiple Proinflammatory Signaling Pathways	111:153	Multiple Proinflammatory Signaling Pathways	111:153	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	1	39	theme	factors	405:411	arg1	regulation					364:373	the comprehensive regulation	346:373	the comprehensive regulation of these important pathogenic factors	346:411	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	4	40	theme	dextran	1004:1010	arg1	DSS					1028:1030	dextran sulfate sodium (DSS)-	1004:1032	dextran sulfate sodium (DSS)-	1004:1032	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	5	41	theme	signals	1358:1364	arg1	suppression					1324:1334	the simultaneous suppression	1307:1334	the simultaneous suppression of JAK/STAT and NF-κB signals	1307:1364	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	2	42	theme	imidazo[4,5-c	502:514	arg1	derivatives					526:536	imidazo[4,5-c]quinoline derivatives	502:536	imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB	502:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	4	43	theme	anti-inflammatory	969:985	arg1	activity					987:994	a strong anti-inflammatory activity	960:994	a strong anti-inflammatory activity	960:994	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	2	44	theme	proinflammatory	593:607	arg1	JAK/STAT					628:635	JAK/STAT	628:635	JAK/STAT	628:635	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	44	theme	proinflammatory	593:607	arg1	pathways					619:626	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	44	theme	proinflammatory	593:607	arg1	NF-κB					641:645	NF-κB	641:645	NF-κB	641:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	3	45	theme	compound	665:672	arg1	8l					674:675	lead compound 8l	660:675	lead compound 8l	660:675	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	0	46	theme	Derivatives	43:53	arg1	Discovery					0:8	Discovery	0:8	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives	0:53	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	3	47	theme	IC50	784:787	arg1	pathways					774:781	NF-κB pathways	768:781	NF-κB pathways (IC50: 150.7 nM)	768:798	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	47	theme	IC50	784:787	arg1	nM					796:797	IC50: 150.7 nM	784:797	IC50: 150.7 nM	784:797	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	2	48	theme	primary	585:591	arg1	JAK/STAT					628:635	JAK/STAT	628:635	JAK/STAT	628:635	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	48	theme	primary	585:591	arg1	pathways					619:626	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	two primary proinflammatory signaling pathways JAK/STAT and NF-κB	581:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	2	48	theme	primary	585:591	arg1	NF-κB					641:645	NF-κB	641:645	NF-κB	641:645	In the current study, we discovered a series of imidazo[4,5-c]quinoline derivatives that potently and simultaneously inhibited two primary proinflammatory signaling pathways JAK/STAT and NF-κB.
36053746	4	49	theme	microbiota	1158:1167	arg1	composition					1139:1149	the structural composition	1124:1149	the structural composition of gut microbiota	1124:1167	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	3	50	theme	lead	660:663	arg1	8l					674:675	lead compound 8l	660:675	lead compound 8l	660:675	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	0	51	theme	Intestinal	169:178	arg1	Homeostasis					180:190	Intestinal Homeostasis	169:190	Intestinal Homeostasis	169:190	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	4	52	theme	gut	1154:1156	arg1	microbiota					1158:1167	gut microbiota	1154:1167	gut microbiota	1154:1167	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	3	53	theme	nanomolar	868:876	arg1	level					878:882	the nanomolar level	864:882	the nanomolar level	864:882	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	1	54	theme	immune	229:234	arg1	factors					249:255	immune inflammatory factors	229:255	immune inflammatory factors	229:255	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	1	55	theme	favorable	424:432	arg1	effect					446:451	a favorable therapeutic effect	422:451	a favorable therapeutic effect	422:451	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	3	56	theme	various	829:835	arg1	TNF-α					914:918	TNF-α	914:918	TNF-α	914:918	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	56	theme	various	829:835	arg1	factors					853:859	various proinflammatory factors	829:859	various proinflammatory factors	829:859	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	56	theme	various	829:835	arg1	IL-12					921:925	IL-12	921:925	IL-12	921:925	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	56	theme	various	829:835	arg1	IL-8					901:904	IL-8	901:904	IL-8	901:904	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	56	theme	various	829:835	arg1	IL-1β					907:911	IL-1β	907:911	IL-1β	907:911	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	56	theme	various	829:835	arg1	IFN-γ					932:936	IFN-γ	932:936	IFN-γ	932:936	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	56	theme	various	829:835	arg1	IL-6					895:898	IL-6	895:898	IL-6	895:898	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	3	57	theme	factors	853:859	arg1	release					818:824	the release	814:824	the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ	814:936	Especially, lead compound 8l showed potent inhibitory activities against interferon-stimulated genes (IC50: 3.3 nM) and NF-κB pathways (IC50: 150.7 nM) and decreased the release of various proinflammatory factors at the nanomolar level, including IL-6, IL-8, IL-1β, TNF-α, IL-12, and IFN-γ.
36053746	1	58	theme	inflammatory	236:247	arg1	factors					249:255	immune inflammatory factors	229:255	immune inflammatory factors	229:255	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	0	59	theme	Inflammatory	64:75	arg1	IBD					92:94	IBD	92:94	IBD	92:94	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	0	59	theme	Inflammatory	64:75	arg1	Disease					83:89	Inflammatory Bowel Disease	64:89	Inflammatory Bowel Disease (IBD)	64:95	Discovery of Novel Imidazo[4,5-c]quinoline Derivatives to Treat Inflammatory Bowel Disease (IBD) by Inhibiting Multiple Proinflammatory Signaling Pathways and Restoring Intestinal Homeostasis.
36053746	1	60	theme	therapeutic	434:444	arg1	effect					446:451	a favorable therapeutic effect	422:451	a favorable therapeutic effect	422:451	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	5	61	theme	JAK/STAT	1339:1346	arg1	signals					1358:1364	JAK/STAT and NF-κB signals	1339:1364	JAK/STAT and NF-κB signals	1339:1364	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	5	62	theme	IBD	1249:1251	arg1	treatment					1236:1244	the treatment	1232:1244	the treatment of IBD	1232:1251	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	1	63	dep	important	384:392	arg1	pathogenic					394:403	pathogenic	394:403	pathogenic	394:403	As a complex pathogenesis driven by immune inflammatory factors and intestinal microbiota, the treatment of inflammatory bowel disease (IBD) may rely on the comprehensive regulation of these important pathogenic factors to reach a favorable therapeutic effect.
36053746	4	64	theme	structural	1128:1137	arg1	composition					1139:1149	the structural composition	1124:1149	the structural composition of gut microbiota	1124:1167	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	5	65	theme	valuable	1204:1211	arg1	compounds					1218:1226	valuable lead compounds	1204:1226	valuable lead compounds	1204:1226	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
36053746	4	66	theme	sodium	1020:1025	arg1	DSS					1028:1030	dextran sulfate sodium (DSS)-	1004:1032	dextran sulfate sodium (DSS)-	1004:1032	In vivo, 8l produced a strong anti-inflammatory activity in both dextran sulfate sodium (DSS)- and 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute enteritis models and restored the structural composition of gut microbiota.
36053746	5	67	theme	lead	1213:1216	arg1	compounds					1218:1226	valuable lead compounds	1204:1226	valuable lead compounds	1204:1226	Collectively, this study provided valuable lead compounds for the treatment of IBD and revealed the great anti-inflammatory potential of the simultaneous suppression of JAK/STAT and NF-κB signals.
35151686	2	0	theme	core	481:484	arg1	structure					486:494	the core structure	477:494	the core structure of GPI glycolipid	477:512	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	5	1	theme	decreased	1076:1084	arg1	activity					1096:1103	decreased locomotor activity	1076:1103	decreased locomotor activity	1076:1103	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	6	2	theme	GPI-GalNAc	1234:1243	arg1	chain					1250:1254	the GPI-GalNAc side chain	1230:1254	the GPI-GalNAc side chain	1230:1254	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	6	3	theme	side	1245:1248	arg1	chain					1250:1254	the GPI-GalNAc side chain	1230:1254	the GPI-GalNAc side chain	1230:1254	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	4	4	from	GPI	908:910	arg1	brains					930:935	PGAP4-KO mouse brains	915:935	PGAP4-KO mouse brains	915:935	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	0	5	theme	bone	71:74	arg1	formation					76:84	bone formation	71:84	bone formation	71:84	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	5	6	theme	locomotor	1086:1094	arg1	activity					1096:1103	decreased locomotor activity	1076:1103	decreased locomotor activity	1076:1103	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	7	7	theme	intracerebral	1433:1445	arg1	inoculation					1447:1457	intracerebral inoculation	1433:1457	intracerebral inoculation of the pathogenic prion strains than wildtype mice	1433:1508	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	7	8	theme	pathogenic	1466:1475	arg1	strains					1483:1489	the pathogenic prion strains	1462:1489	the pathogenic prion strains	1462:1489	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	1	9	theme	lipid	270:274	arg1	rafts					276:280	lipid rafts	270:280	lipid rafts	270:280	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	0	10	theme	brain	90:94	arg1	functions					96:104	brain functions	90:104	brain functions	90:104	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	4	11	theme	Pgap4	755:759	arg1	mRNA					761:764	Pgap4 mRNA	755:764	Pgap4 mRNA	755:764	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	4	12	theme	spectrometry	834:845	arg1	analysis					847:854	mass spectrometry analysis	829:854	mass spectrometry analysis	829:854	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	4	13	theme	PrPC	903:906	arg1	GPI					908:910	PrPC GPI	903:910	PrPC GPI in PGAP4-KO mouse brains	903:935	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	4	14	theme	chain	894:898	arg1	loss					870:873	the loss	866:873	the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains	866:935	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	4	15	theme	mass	829:832	arg1	analysis					847:854	mass spectrometry analysis	829:854	mass spectrometry analysis	829:854	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	6	16	theme	in vivo	1272:1278	arg1	functions					1280:1288	in vivo functions	1272:1288	in vivo functions of GPI-APs in mammals, especially in bone and the brain	1272:1344	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	5	17	theme	raft	1170:1173	arg1	association					1175:1185	lipid raft association	1164:1185	lipid raft association of various GPI-APs	1164:1204	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	2	18	theme	glycolipid	503:512	arg1	structure					486:494	the core structure	477:494	the core structure of GPI glycolipid	477:512	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	5	19	theme	impaired	1051:1058	arg1	formation					1065:1073	impaired bone formation	1051:1073	impaired bone formation	1051:1073	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	7	20	theme	chain	1564:1568	arg1	roles					1539:1543	the protective roles	1524:1543	the protective roles of the GalNAc side chain against prion diseases	1524:1591	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	2	21	theme	GPI	499:501	arg1	glycolipid					503:512	GPI glycolipid	499:512	GPI glycolipid	499:512	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	5	22	theme	bone	1060:1063	arg1	formation					1065:1073	impaired bone formation	1051:1073	impaired bone formation	1051:1073	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	6	23	theme	GPI-APs	1293:1299	arg1	functions					1280:1288	in vivo functions	1272:1288	in vivo functions of GPI-APs in mammals, especially in bone and the brain	1272:1344	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	6	24	from	functions	1280:1288	arg1	mammals					1304:1310	mammals	1304:1310	mammals	1304:1310	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	6	24	from	functions	1280:1288	arg1	bone					1327:1330	bone	1327:1330	bone	1327:1330	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	6	24	from	functions	1280:1288	arg1	brain					1340:1344	the brain	1336:1344	the brain	1336:1344	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	7	25	theme	PGAP4-KO	1357:1364	arg1	mice					1366:1369	PGAP4-KO mice	1357:1369	PGAP4-KO mice	1357:1369	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	6	26	from	bone	1327:1330	arg1	functions					1280:1288	in vivo functions	1272:1288	in vivo functions of GPI-APs in mammals, especially in bone and the brain	1272:1344	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	3	27	theme	initiation	707:716	arg1	PGAP4					726:730	PGAP4	726:730	PGAP4	726:730	Here, we investigated the physiological and pathological roles of the GPI-GalNAc side chain in vivo by knocking out its initiation enzyme, PGAP4, in mice.
35151686	3	27	theme	initiation	707:716	arg1	enzyme					718:723	its initiation enzyme	703:723	its initiation enzyme	703:723	Here, we investigated the physiological and pathological roles of the GPI-GalNAc side chain in vivo by knocking out its initiation enzyme, PGAP4, in mice.
35151686	6	28	from	brain	1340:1344	arg1	functions					1280:1288	in vivo functions	1272:1288	in vivo functions of GPI-APs in mammals, especially in bone and the brain	1272:1344	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	6	29	from	GPI-APs	1293:1299	arg1	mammals					1304:1310	mammals	1304:1310	mammals	1304:1310	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	6	29	from	GPI-APs	1293:1299	arg1	bone					1327:1330	bone	1327:1330	bone	1327:1330	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	6	29	from	GPI-APs	1293:1299	arg1	brain					1340:1344	the brain	1336:1344	the brain	1336:1344	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	4	30	theme	side	889:892	arg1	chain					894:898	the GalNAc side chain	878:898	the GalNAc side chain	878:898	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	7	31	theme	prion	1477:1481	arg1	strains					1483:1489	the pathogenic prion strains	1462:1489	the pathogenic prion strains	1462:1489	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	5	32	theme	elevated	1008:1015	arg1	level					1044:1048	an elevated blood alkaline phosphatase level	1005:1048	an elevated blood alkaline phosphatase level	1005:1048	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	2	33	theme	GPI-anchored	317:328	arg1	PrPC					378:381	the prion protein PrPC	360:381	the prion protein PrPC	360:381	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	33	theme	GPI-anchored	317:328	arg1	GPI-APs					340:346	GPI-APs	340:346	GPI-APs	340:346	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	33	theme	GPI-anchored	317:328	arg1	proteins					330:337	some GPI-anchored proteins	312:337	some GPI-anchored proteins (GPI-APs)	312:347	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	7	34	theme	strains	1483:1489	arg1	inoculation					1447:1457	intracerebral inoculation	1433:1457	intracerebral inoculation of the pathogenic prion strains than wildtype mice	1433:1508	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	0	35	theme	N-acetylgalactosamine	12:32	arg1	chain					39:43	the N-acetylgalactosamine side chain	8:43	the N-acetylgalactosamine side chain of the GPI-anchor	8:61	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	1	36	theme	cell	289:292	arg1	surface					294:300	the cell surface	285:300	the cell surface	285:300	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	2	37	theme	GPI-GalNAc	541:550	arg1	chain					557:561	this GPI-GalNAc side chain	536:561	this GPI-GalNAc side chain	536:561	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	4	38	from	loss	870:873	arg1	GPI					908:910	PrPC GPI	903:910	PrPC GPI in PGAP4-KO mouse brains	903:935	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	6	39	from	mammals	1304:1310	arg1	functions					1280:1288	in vivo functions	1272:1288	in vivo functions of GPI-APs in mammals, especially in bone and the brain	1272:1344	Thus, we conclude that the GPI-GalNAc side chain is required for in vivo functions of GPI-APs in mammals, especially in bone and the brain.
35151686	1	40	theme	posttranslational	191:207	arg1	Glycosylphosphatidylinositol					151:178	Glycosylphosphatidylinositol	151:178	Glycosylphosphatidylinositol (GPI)	151:184	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	1	40	theme	posttranslational	191:207	arg1	modification					220:231	a posttranslational glycolipid modification	189:231	a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface	189:300	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	5	41	theme	normal	1135:1140	arg1	levels					1153:1158	normal expression levels	1135:1158	normal expression levels	1135:1158	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	5	42	theme	blood	1017:1021	arg1	level					1044:1048	an elevated blood alkaline phosphatase level	1005:1048	an elevated blood alkaline phosphatase level	1005:1048	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	7	43	theme	protective	1528:1537	arg1	roles					1539:1543	the protective roles	1524:1543	the protective roles of the GalNAc side chain against prion diseases	1524:1591	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	1	44	theme	glycolipid	209:218	arg1	Glycosylphosphatidylinositol					151:178	Glycosylphosphatidylinositol	151:178	Glycosylphosphatidylinositol (GPI)	151:184	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	1	44	theme	glycolipid	209:218	arg1	modification					220:231	a posttranslational glycolipid modification	189:231	a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface	189:300	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	0	45	theme	chain	39:43	arg1	Loss					0:3	Loss	0:3	Loss of the N-acetylgalactosamine side chain of the GPI-anchor	0:61	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	5	46	theme	expression	1142:1151	arg1	levels					1153:1158	normal expression levels	1135:1158	normal expression levels	1135:1158	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	0	47	theme	disease	132:138	arg1	pathology					140:148	the prion disease pathology	122:148	the prion disease pathology	122:148	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	5	48	theme	alkaline	1023:1030	arg1	level					1044:1048	an elevated blood alkaline phosphatase level	1005:1048	an elevated blood alkaline phosphatase level	1005:1048	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	2	49	theme	side	398:401	arg1	chain					403:407	a glycan side chain	389:407	a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid	389:472	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	0	50	theme	side	34:37	arg1	chain					39:43	the N-acetylgalactosamine side chain	8:43	the N-acetylgalactosamine side chain of the GPI-anchor	8:61	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	4	51	theme	mouse	924:928	arg1	brains					930:935	PGAP4-KO mouse brains	915:935	PGAP4-KO mouse brains	915:935	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	0	52	theme	prion	126:130	arg1	pathology					140:148	the prion disease pathology	122:148	the prion disease pathology	122:148	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	2	53	contain	have	384:387	arg1	PrPC					378:381	the prion protein PrPC	360:381	the prion protein PrPC	360:381	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	53	contain	have	384:387	arg2	chain					403:407	a glycan side chain	389:407	a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid	389:472	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	53	contain	have	384:387	arg1	GPI-APs					340:346	GPI-APs	340:346	GPI-APs	340:346	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	53	contain	have	384:387	arg1	proteins					330:337	some GPI-anchored proteins	312:337	some GPI-anchored proteins (GPI-APs)	312:347	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	54	theme	glycan	391:396	arg1	chain					403:407	a glycan side chain	389:407	a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid	389:472	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	55	theme	-galactose-sialic	451:467	arg1	acid					469:472	N-acetylgalactosamine (GalNAc)-galactose-sialic acid	421:472	N-acetylgalactosamine (GalNAc)-galactose-sialic acid	421:472	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	2	56	theme	in vivo	515:521	arg1	functions					523:531	in vivo functions	515:531	in vivo functions of this GPI-GalNAc side chain	515:561	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	3	57	theme	pathological	631:642	arg1	roles					644:648	the physiological and pathological roles	609:648	the physiological and pathological roles of the GPI-GalNAc side chain in vivo	609:685	Here, we investigated the physiological and pathological roles of the GPI-GalNAc side chain in vivo by knocking out its initiation enzyme, PGAP4, in mice.
35151686	5	58	theme	various	975:981	arg1	memory					1119:1124	impaired memory	1110:1124	impaired memory	1110:1124	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	5	58	theme	various	975:981	arg1	level					1044:1048	an elevated blood alkaline phosphatase level	1005:1048	an elevated blood alkaline phosphatase level	1005:1048	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	5	58	theme	various	975:981	arg1	activity					1096:1103	decreased locomotor activity	1076:1103	decreased locomotor activity	1076:1103	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	5	58	theme	various	975:981	arg1	formation					1065:1073	impaired bone formation	1051:1073	impaired bone formation	1051:1073	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	5	58	theme	various	975:981	arg1	phenotypes					983:992	various phenotypes	975:992	various phenotypes	975:992	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	4	59	theme	GalNAc	882:887	arg1	chain					894:898	the GalNAc side chain	878:898	the GalNAc side chain	878:898	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	5	60	theme	impaired	1110:1117	arg1	memory					1119:1124	impaired memory	1110:1124	impaired memory	1110:1124	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	1	61	from	proteins	258:265	arg1	rafts					276:280	lipid rafts	270:280	lipid rafts	270:280	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	3	62	theme	side	668:671	arg1	chain					673:677	the GPI-GalNAc side chain in vivo	653:685	the GPI-GalNAc side chain in vivo	653:685	Here, we investigated the physiological and pathological roles of the GPI-GalNAc side chain in vivo by knocking out its initiation enzyme, PGAP4, in mice.
35151686	5	63	theme	phosphatase	1032:1042	arg1	level					1044:1048	an elevated blood alkaline phosphatase level	1005:1048	an elevated blood alkaline phosphatase level	1005:1048	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	7	64	theme	prion	1578:1582	arg1	diseases					1584:1591	prion diseases	1578:1591	prion diseases	1578:1591	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	2	65	theme	chain	557:561	arg1	functions					523:531	in vivo functions	515:531	in vivo functions of this GPI-GalNAc side chain	515:561	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	3	66	theme	chain	673:677	arg1	roles					644:648	the physiological and pathological roles	609:648	the physiological and pathological roles of the GPI-GalNAc side chain in vivo	609:685	Here, we investigated the physiological and pathological roles of the GPI-GalNAc side chain in vivo by knocking out its initiation enzyme, PGAP4, in mice.
35151686	5	67	theme	lipid	1164:1168	arg1	association					1175:1185	lipid raft association	1164:1185	lipid raft association of various GPI-APs	1164:1204	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	7	68	theme	prion	1395:1399	arg1	diseases					1401:1408	prion diseases	1395:1408	prion diseases	1395:1408	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	1	69	theme	proteins	236:243	arg1	Glycosylphosphatidylinositol					151:178	Glycosylphosphatidylinositol	151:178	Glycosylphosphatidylinositol (GPI)	151:184	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	1	69	theme	proteins	236:243	arg1	modification					220:231	a posttranslational glycolipid modification	189:231	a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface	189:300	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	2	70	theme	side	552:555	arg1	chain					557:561	this GPI-GalNAc side chain	536:561	this GPI-GalNAc side chain	536:561	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	3	71	theme	physiological	613:625	arg1	roles					644:648	the physiological and pathological roles	609:648	the physiological and pathological roles of the GPI-GalNAc side chain in vivo	609:685	Here, we investigated the physiological and pathological roles of the GPI-GalNAc side chain in vivo by knocking out its initiation enzyme, PGAP4, in mice.
35151686	7	72	theme	side	1559:1562	arg1	chain					1564:1568	the GalNAc side chain	1548:1568	the GalNAc side chain	1548:1568	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	1	73	mod	modification	220:231	arg1	proteins					236:243	proteins	236:243	proteins	236:243	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	1	73	mod	modification	220:231	arg3	glycolipid					209:218	a posttranslational glycolipid modification	189:231	a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface	189:300	Glycosylphosphatidylinositol (GPI) is a posttranslational glycolipid modification of proteins that anchors proteins in lipid rafts on the cell surface.
35151686	4	74	theme	PGAP4-KO	915:922	arg1	brains					930:935	PGAP4-KO mouse brains	915:935	PGAP4-KO mouse brains	915:935	We show that Pgap4 mRNA is highly expressed in the brain, particularly in neurons, and mass spectrometry analysis confirmed the loss of the GalNAc side chain in PrPC GPI in PGAP4-KO mouse brains.
35151686	3	75	theme	GPI-GalNAc	657:666	arg1	chain					673:677	the GPI-GalNAc side chain in vivo	653:685	the GPI-GalNAc side chain in vivo	653:685	Here, we investigated the physiological and pathological roles of the GPI-GalNAc side chain in vivo by knocking out its initiation enzyme, PGAP4, in mice.
35151686	7	76	theme	wildtype	1496:1503	arg1	mice					1505:1508	wildtype mice	1496:1508	wildtype mice	1496:1508	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	2	77	theme	protein	370:376	arg1	PrPC					378:381	the prion protein PrPC	360:381	the prion protein PrPC	360:381	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	5	78	theme	various	1190:1196	arg1	GPI-APs					1198:1204	various GPI-APs	1190:1204	various GPI-APs	1190:1204	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	0	79	theme	GPI-anchor	52:61	arg1	chain					39:43	the N-acetylgalactosamine side chain	8:43	the N-acetylgalactosamine side chain of the GPI-anchor	8:61	Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology.
35151686	5	80	theme	PGAP4-KO	951:958	arg1	mice					960:963	PGAP4-KO mice	951:963	PGAP4-KO mice	951:963	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	2	81	theme	prion	364:368	arg1	PrPC					378:381	the prion protein PrPC	360:381	the prion protein PrPC	360:381	Although some GPI-anchored proteins (GPI-APs), including the prion protein PrPC, have a glycan side chain composed of N-acetylgalactosamine (GalNAc)-galactose-sialic acid on the core structure of GPI glycolipid, in vivo functions of this GPI-GalNAc side chain are largely unresolved.
35151686	7	82	theme	GalNAc	1552:1557	arg1	chain					1564:1568	the GalNAc side chain	1548:1568	the GalNAc side chain	1548:1568	Moreover, PGAP4-KO mice were more vulnerable to prion diseases and died earlier after intracerebral inoculation of the pathogenic prion strains than wildtype mice, highlighting the protective roles of the GalNAc side chain against prion diseases.
35151686	5	83	theme	GPI-APs	1198:1204	arg1	levels					1153:1158	normal expression levels	1135:1158	normal expression levels	1135:1158	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35151686	5	83	theme	GPI-APs	1198:1204	arg1	association					1175:1185	lipid raft association	1164:1185	lipid raft association of various GPI-APs	1164:1204	Furthermore, PGAP4-KO mice exhibited various phenotypes, including an elevated blood alkaline phosphatase level, impaired bone formation, decreased locomotor activity, and impaired memory, despite normal expression levels and lipid raft association of various GPI-APs.
35387258	6	0	dep	components	1209:1218	arg1	the					1195:1197	the	1195:1197	the	1195:1197	At the same time, combined with network pharmacology and molecular docking, the effective components and pathways of rhubarb involved in inhibiting VEI were screened.
35387258	9	1	theme	ion	1608:1610	arg1	mode					1612:1615	the negative ion mode	1595:1615	the negative ion mode	1595:1615	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	4	2	theme	rhubarb	1003:1009	arg1	species					992:998	three species	986:998	three species of rhubarb	986:1009	Methods Plant metabolomics was used to compare the chemical components of three species of rhubarb.
35387258	2	3	theme	VEI	598:600	arg1	mechanism					566:574	mechanism	566:574	mechanism	566:574	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	2	3	theme	VEI	598:600	arg1	components					551:560	effective components	541:560	effective components	541:560	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	3	4	from	effect	897:902	arg1	VEI					907:909	VEI	907:909	VEI	907:909	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	10	5	theme	rhubarb	1720:1726	arg1	species					1709:1715	the three species	1699:1715	the three species of rhubarb	1699:1726	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	2	6	dep	components	551:560	arg1	the					537:539	the	537:539	the	537:539	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	11	7	theme	LPS-induced	2045:2055	arg1	cells					2066:2070	LPS-induced EA.hy926 cells	2045:2070	LPS-induced EA.hy926 cells	2045:2070	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	6	8	theme	effective	1199:1207	arg1	components					1209:1218	effective components	1199:1218	effective components	1199:1218	At the same time, combined with network pharmacology and molecular docking, the effective components and pathways of rhubarb involved in inhibiting VEI were screened.
35387258	3	9	from	rhubarb	714:720	arg1	VEI					753:755	VEI	753:755	VEI	753:755	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	11	10	from	response	2033:2040	arg1	cells					2066:2070	LPS-induced EA.hy926 cells	2045:2070	LPS-induced EA.hy926 cells	2045:2070	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	11	dep	cytokines	2113:2121	arg1	IL-1β					2129:2133	IL-1β	2129:2133	IL-1β	2129:2133	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	11	dep	cytokines	2113:2121	arg1	TNF-α					2140:2144	TNF-α	2140:2144	TNF-α	2140:2144	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	11	dep	cytokines	2113:2121	arg1	IL-6					2123:2126	IL-6	2123:2126	IL-6	2123:2126	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	11	dep	cytokines	2113:2121	arg1	cytokines					2113:2121	inflammatory cytokines	2100:2121	inflammatory cytokines IL-6, IL-1β, and TNF-α	2100:2144	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	8	12	from	differences	1392:1402	arg1	distribution					1411:1422	the distribution	1407:1422	the distribution of chemical components among the three species of rhubarb	1407:1480	Results There were significant differences in the distribution of chemical components among the three species of rhubarb.
35387258	7	13	theme	molecular	1342:1350	arg1	biology					1352:1358	molecular biology	1342:1358	molecular biology	1342:1358	The mechanism of rhubarb inhibiting VEI was verified by molecular biology.
35387258	11	14	theme	p65	2193:2195	arg1	expressions					2161:2171	expressions	2161:2171	expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation	2161:2291	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	10	15	dep	factors	1766:1772	arg1	such					1775:1778	such	1775:1778	such	1775:1778	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	10	16	theme	inhibitory	1866:1875	arg1	effect					1877:1882	the best inhibitory effect	1857:1882	the best inhibitory effect on VEI	1857:1889	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	0	17	theme	Pathway	124:130	arg1	Regulation					85:94	Regulation	85:94	Regulation of PI3K/AKT/NF-κB Signaling Pathway	85:130	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.
35387258	4	18	used	used	943:946	arg2	metabolomics					926:937	Methods Plant metabolomics	912:937	Methods Plant metabolomics	912:937	Methods Plant metabolomics was used to compare the chemical components of three species of rhubarb.
35387258	5	19	theme	species	1034:1040	arg1	efficacy					1016:1023	The efficacy	1012:1023	The efficacy of three species of rhubarb in inhibiting VEI	1012:1069	The efficacy of three species of rhubarb in inhibiting VEI was compared through cell experiments in vitro.
35387258	13	20	theme	oxidative	2478:2486	arg1	stress					2488:2493	inflammation-induced cellular oxidative stress	2448:2493	inflammation-induced cellular oxidative stress	2448:2493	All results indicated that the process of inflammation-induced cellular oxidative stress, which resulted in VEI, was obviously improved by three species of rhubarb.
35387258	2	21	theme	rhubarb	579:585	arg1	VEI					598:600	rhubarb inhibiting VEI	579:600	rhubarb inhibiting VEI	579:600	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	7	22	theme	VEI	1322:1324	arg1	mechanism					1290:1298	The mechanism	1286:1298	The mechanism of rhubarb inhibiting VEI	1286:1324	The mechanism of rhubarb inhibiting VEI was verified by molecular biology.
35387258	3	23	from	species	703:709	arg1	VEI					753:755	VEI	753:755	VEI	753:755	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	14	24	theme	other	2762:2766	arg1	components					2778:2787	other potential components	2762:2787	other potential components	2762:2787	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	10	25	theme	inflammatory	1753:1764	arg1	factors					1766:1772	inflammatory factors	1753:1772	inflammatory factors (such as IL-1β, IL-6, and TNF-α)	1753:1805	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	11	26	theme	rhubarb	1997:2003	arg1	species					1986:1992	Three species	1980:1992	Three species of rhubarb	1980:2003	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	6	27	theme	network	1151:1157	arg1	pharmacology					1159:1170	network pharmacology	1151:1170	network pharmacology	1151:1170	At the same time, combined with network pharmacology and molecular docking, the effective components and pathways of rhubarb involved in inhibiting VEI were screened.
35387258	7	28	theme	rhubarb	1303:1309	arg1	VEI					1322:1324	rhubarb inhibiting VEI	1303:1324	rhubarb inhibiting VEI	1303:1324	The mechanism of rhubarb inhibiting VEI was verified by molecular biology.
35387258	12	29	from	contents	2349:2356	arg1	cells					2384:2388	EA.hy926 cells	2375:2388	EA.hy926 cells induced by LPS	2375:2403	In addition, three species of rhubarb could lessen the contents of ROS and NO in EA.hy926 cells induced by LPS.
35387258	3	30	theme	material	779:786	arg1	basis					788:792	the material basis	775:792	the material basis	775:792	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	14	31	theme	rhubarb	2638:2644	arg1	species					2627:2633	three species	2621:2633	three species of rhubarb	2621:2644	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	0	32	theme	Vascular	53:60	arg1	Injury					74:79	Vascular Endothelial Injury	53:79	Vascular Endothelial Injury	53:79	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.
35387258	10	33	theme	efficacy	1925:1932	arg1	light					1898:1902	the light	1894:1902	the light of the comprehensive efficacy	1894:1932	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	9	34	from	components	1519:1528	arg1	mode					1612:1615	the negative ion mode	1595:1615	the negative ion mode	1595:1615	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	9	34	from	components	1519:1528	arg1	mode					1550:1553	the positive ion mode	1533:1553	the positive ion mode	1533:1553	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	2	35	theme	vascular	498:505	arg1	VEI					527:529	VEI	527:529	VEI	527:529	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	2	35	theme	vascular	498:505	arg1	injury					519:524	vascular endothelial injury	498:524	vascular endothelial injury (VEI)	498:530	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	3	36	dep	species	818:824	arg1	inhibit					829:835	inhibit	829:835	to inhibit VEI	826:839	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	3	36	dep	species	818:824	arg1	elucidate					849:857	elucidate	849:857	to elucidate the mechanism of rhubarb's inhibitory effect on VEI	846:909	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	1	37	theme	Chinese	171:177	arg1	medicine					179:186	a traditional Chinese medicine	157:186	a traditional Chinese medicine derived from three species	157:213	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	1	37	theme	Chinese	171:177	arg1	Rhubarb					148:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	14	38	theme	NO	2882:2883	arg1	contents					2806:2813	the contents	2802:2813	the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO	2802:2883	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	5	39	theme	rhubarb	1045:1051	arg1	species					1034:1040	three species	1028:1040	three species of rhubarb	1028:1051	The efficacy of three species of rhubarb in inhibiting VEI was compared through cell experiments in vitro.
35387258	10	40	from	effect	1877:1882	arg1	VEI					1887:1889	VEI	1887:1889	VEI	1887:1889	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	9	41	theme	chemical	1510:1517	arg1	components					1519:1528	36 different chemical components	1497:1528	36 different chemical components in the positive ion mode	1497:1553	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	4	42	theme	Plant	920:924	arg1	metabolomics					926:937	Methods Plant metabolomics	912:937	Methods Plant metabolomics	912:937	Methods Plant metabolomics was used to compare the chemical components of three species of rhubarb.
35387258	12	43	theme	NO	2369:2370	arg1	contents					2349:2356	the contents	2345:2356	the contents of ROS and NO in EA.hy926 cells induced by LPS	2345:2403	In addition, three species of rhubarb could lessen the contents of ROS and NO in EA.hy926 cells induced by LPS.
35387258	0	44	theme	PI3K/AKT/NF-κB	99:112	arg1	Pathway					124:130	PI3K/AKT/NF-κB Signaling Pathway	99:130	PI3K/AKT/NF-κB Signaling Pathway	99:130	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.
35387258	9	45	from	components	1581:1590	arg1	mode					1612:1615	the negative ion mode	1595:1615	the negative ion mode	1595:1615	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	9	45	from	components	1581:1590	arg1	mode					1550:1553	the positive ion mode	1533:1553	the positive ion mode	1533:1553	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	14	46	theme	signaling	2918:2926	arg1	pathway					2928:2934	the PI3K/AKT/NF-κB signaling pathway	2899:2934	the PI3K/AKT/NF-κB signaling pathway	2899:2934	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	13	47	theme	cellular	2469:2476	arg1	stress					2488:2493	inflammation-induced cellular oxidative stress	2448:2493	inflammation-induced cellular oxidative stress	2448:2493	All results indicated that the process of inflammation-induced cellular oxidative stress, which resulted in VEI, was obviously improved by three species of rhubarb.
35387258	9	48	theme	positive	1537:1544	arg1	mode					1550:1553	the positive ion mode	1533:1553	the positive ion mode	1533:1553	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	12	49	theme	ROS	2361:2363	arg1	contents					2349:2356	the contents	2345:2356	the contents of ROS and NO in EA.hy926 cells induced by LPS	2345:2403	In addition, three species of rhubarb could lessen the contents of ROS and NO in EA.hy926 cells induced by LPS.
35387258	10	50	dep	R.	1839:1840	arg1	tanguticum					1842:1851	R. tanguticum	1839:1851	R. tanguticum	1839:1851	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	13	51	theme	stress	2488:2493	arg1	process					2437:2443	the process	2433:2443	the process of inflammation-induced cellular oxidative stress, which resulted in VEI,	2433:2517	All results indicated that the process of inflammation-induced cellular oxidative stress, which resulted in VEI, was obviously improved by three species of rhubarb.
35387258	1	52	theme	blood	299:303	arg1	stasis					305:310	blood stasis	299:310	blood stasis based on its traditional effects of removing blood stasis and dredging the meridians	299:395	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	14	53	theme	inflammatory	2818:2829	arg1	factors					2831:2837	inflammatory factors	2818:2837	inflammatory factors (such as IL-1β, IL-6, and TNF-α)	2818:2870	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	11	54	theme	inflammatory	2100:2111	arg1	IL-1β					2129:2133	IL-1β	2129:2133	IL-1β	2129:2133	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	54	theme	inflammatory	2100:2111	arg1	TNF-α					2140:2144	TNF-α	2140:2144	TNF-α	2140:2144	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	54	theme	inflammatory	2100:2111	arg1	IL-6					2123:2126	IL-6	2123:2126	IL-6	2123:2126	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	54	theme	inflammatory	2100:2111	arg1	cytokines					2113:2121	inflammatory cytokines	2100:2121	inflammatory cytokines IL-6, IL-1β, and TNF-α	2100:2144	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	14	55	theme	R.	2582:2583	arg1	tanguticum					2585:2594	Conclusion R. tanguticum	2571:2594	Conclusion R. tanguticum	2571:2594	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	14	56	theme	PI3K/AKT/NF-κB	2903:2916	arg1	pathway					2928:2934	the PI3K/AKT/NF-κB signaling pathway	2899:2934	the PI3K/AKT/NF-κB signaling pathway	2899:2934	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	9	57	theme	chemical	1572:1579	arg1	components					1581:1590	38 different chemical components	1559:1590	38 different chemical components in the negative ion mode	1559:1615	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	11	58	theme	STAT3	2202:2206	arg1	protein					2208:2214	STAT3 protein	2202:2214	STAT3 protein	2202:2214	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	3	59	from	differences	653:663	arg1	compositions					677:688	chemical compositions	668:688	chemical compositions	668:688	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	9	60	theme	negative	1599:1606	arg1	mode					1612:1615	the negative ion mode	1595:1615	the negative ion mode	1595:1615	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	11	61	theme	PI3K/AKT/NF-κB	2223:2236	arg1	pathway					2238:2244	the PI3K/AKT/NF-κB pathway	2219:2244	the PI3K/AKT/NF-κB pathway	2219:2244	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	3	62	theme	species	703:709	arg1	compositions					677:688	chemical compositions	668:688	chemical compositions	668:688	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	6	63	theme	rhubarb	1236:1242	arg1	pathways					1224:1231	pathways	1224:1231	pathways	1224:1231	At the same time, combined with network pharmacology and molecular docking, the effective components and pathways of rhubarb involved in inhibiting VEI were screened.
35387258	6	63	theme	rhubarb	1236:1242	arg1	components					1209:1218	effective components	1199:1218	effective components	1199:1218	At the same time, combined with network pharmacology and molecular docking, the effective components and pathways of rhubarb involved in inhibiting VEI were screened.
35387258	11	64	theme	inflammatory	2020:2031	arg1	response					2033:2040	the inflammatory response	2016:2040	the inflammatory response in LPS-induced EA.hy926 cells	2016:2070	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	14	65	theme	blood	2983:2987	arg1	vessels					2989:2995	the blood vessels	2979:2995	the blood vessels	2979:2995	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	1	66	theme	blood	268:272	arg1	circulation					274:284	blood circulation	268:284	blood circulation	268:284	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	2	67	theme	inhibiting	587:596	arg1	VEI					598:600	rhubarb inhibiting VEI	579:600	rhubarb inhibiting VEI	579:600	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	6	68	theme	same	1126:1129	arg1	time					1131:1134	the same time	1122:1134	the same time	1122:1134	At the same time, combined with network pharmacology and molecular docking, the effective components and pathways of rhubarb involved in inhibiting VEI were screened.
35387258	11	69	theme	PI3K	2176:2179	arg1	expressions					2161:2171	expressions	2161:2171	expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation	2161:2291	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	14	70	theme	factors	2831:2837	arg1	contents					2806:2813	the contents	2802:2813	the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO	2802:2883	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	13	71	theme	rhubarb	2562:2568	arg1	species					2551:2557	three species	2545:2557	three species of rhubarb	2545:2568	All results indicated that the process of inflammation-induced cellular oxidative stress, which resulted in VEI, was obviously improved by three species of rhubarb.
35387258	11	72	theme	EA.hy926	2057:2064	arg1	cells					2066:2070	LPS-induced EA.hy926 cells	2045:2070	LPS-induced EA.hy926 cells	2045:2070	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	12	73	theme	EA.hy926	2375:2382	arg1	cells					2384:2388	EA.hy926 cells	2375:2388	EA.hy926 cells induced by LPS	2375:2403	In addition, three species of rhubarb could lessen the contents of ROS and NO in EA.hy926 cells induced by LPS.
35387258	3	74	theme	rhubarb	714:720	arg1	species					703:709	the three species	693:709	the three species of rhubarb and their inhibitory effect on VEI	693:755	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	14	75	theme	vascular	2954:2961	arg1	endothelium					2963:2973	the vascular endothelium	2950:2973	the vascular endothelium	2950:2973	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	11	76	theme	NF-κB	2187:2191	arg1	p65					2193:2195	NF-κB p65	2187:2195	NF-κB p65	2187:2195	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	77	dep	pathway	2238:2244	arg1	phosphorylation					2277:2291	phosphorylation	2277:2291	phosphorylation	2277:2291	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	2	78	theme	effective	541:549	arg1	components					551:560	effective components	541:560	effective components	541:560	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	10	79	contain	had	1853:1855	arg1	R.					1839:1840	R.	1839:1840	R.	1839:1840	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	10	79	contain	had	1853:1855	arg2	effect					1877:1882	the best inhibitory effect	1857:1882	the best inhibitory effect on VEI	1857:1889	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	10	80	theme	best	1861:1864	arg1	effect					1877:1882	the best inhibitory effect	1857:1882	the best inhibitory effect on VEI	1857:1889	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	10	81	dep	R.	1949:1950	arg1	palmatum					1952:1959	R. palmatum	1949:1959	R. palmatum	1949:1959	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	10	81	dep	R.	1949:1950	arg1	officinale					1968:1977	officinale	1968:1977	officinale	1968:1977	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	6	82	theme	molecular	1176:1184	arg1	docking					1186:1192	molecular docking	1176:1192	molecular docking	1176:1192	At the same time, combined with network pharmacology and molecular docking, the effective components and pathways of rhubarb involved in inhibiting VEI were screened.
35387258	14	83	theme	potential	2768:2776	arg1	components					2778:2787	other potential components	2762:2787	other potential components	2762:2787	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	10	84	theme	factors	1766:1772	arg1	contents					1741:1748	the contents	1737:1748	the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α)	1737:1805	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	5	85	theme	cell	1092:1095	arg1	experiments					1097:1107	cell experiments	1092:1107	cell experiments	1092:1107	The efficacy of three species of rhubarb in inhibiting VEI was compared through cell experiments in vitro.
35387258	8	86	theme	components	1436:1445	arg1	distribution					1411:1422	the distribution	1407:1422	the distribution of chemical components among the three species of rhubarb	1407:1480	Results There were significant differences in the distribution of chemical components among the three species of rhubarb.
35387258	11	87	from	expressions	2161:2171	arg1	inhibition					2254:2263	the inhibition	2250:2263	the inhibition of proteins	2250:2275	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	87	from	expressions	2161:2171	arg1	pathway					2238:2244	the PI3K/AKT/NF-κB pathway	2219:2244	the PI3K/AKT/NF-κB pathway	2219:2244	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	1	88	theme	Background/Aim	133:146	arg1	medicine					179:186	a traditional Chinese medicine	157:186	a traditional Chinese medicine derived from three species	157:213	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	1	88	theme	Background/Aim	133:146	arg1	Rhubarb					148:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	14	89	theme	gallic	2675:2680	arg1	acid					2682:2685	gallic acid	2675:2685	gallic acid	2675:2685	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	7	90	theme	inhibiting	1311:1320	arg1	VEI					1322:1324	rhubarb inhibiting VEI	1303:1324	rhubarb inhibiting VEI	1303:1324	The mechanism of rhubarb inhibiting VEI was verified by molecular biology.
35387258	8	91	theme	chemical	1427:1434	arg1	components					1436:1445	chemical components	1427:1445	chemical components	1427:1445	Results There were significant differences in the distribution of chemical components among the three species of rhubarb.
35387258	10	92	theme	comprehensive	1911:1923	arg1	efficacy					1925:1932	the comprehensive efficacy	1907:1932	the comprehensive efficacy	1907:1932	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	0	93	theme	Endothelial	62:72	arg1	Injury					74:79	Vascular Endothelial Injury	53:79	Vascular Endothelial Injury	53:79	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.
35387258	8	94	dep	Results	1361:1367	arg1	were					1375:1378	were	1375:1378	were significant differences in the distribution of chemical components among the three species of rhubarb	1375:1480	Results There were significant differences in the distribution of chemical components among the three species of rhubarb.
35387258	2	95	theme	endothelial	507:517	arg1	VEI					527:529	VEI	527:529	VEI	527:529	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	2	95	theme	endothelial	507:517	arg1	injury					519:524	vascular endothelial injury	498:524	vascular endothelial injury (VEI)	498:530	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	3	96	theme	inhibitory	732:741	arg1	effect					743:748	their inhibitory effect	726:748	their inhibitory effect on VEI	726:755	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	1	97	theme	traditional	159:169	arg1	medicine					179:186	a traditional Chinese medicine	157:186	a traditional Chinese medicine derived from three species	157:213	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	1	97	theme	traditional	159:169	arg1	Rhubarb					148:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	8	98	theme	rhubarb	1474:1480	arg1	species					1463:1469	the three species	1453:1469	the three species of rhubarb	1453:1480	Results There were significant differences in the distribution of chemical components among the three species of rhubarb.
35387258	14	99	theme	procyanidin	2721:2731	arg1	B-2,3,3'-di-O-gallatein					2733:2755	procyanidin B-2,3,3'-di-O-gallatein	2721:2755	procyanidin B-2,3,3'-di-O-gallatein	2721:2755	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	3	100	theme	dominant	809:816	arg1	species					818:824	the dominant species	805:824	the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI	805:909	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	1	101	theme	blood	357:361	arg1	stasis					363:368	blood stasis	357:368	blood stasis	357:368	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	14	102	dep	factors	2831:2837	arg1	such					2840:2843	such	2840:2843	such	2840:2843	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	9	103	theme	different	1500:1508	arg1	components					1519:1528	36 different chemical components	1497:1528	36 different chemical components in the positive ion mode	1497:1553	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	11	104	theme	AKT	2182:2184	arg1	expressions					2161:2171	expressions	2161:2171	expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation	2161:2291	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	0	105	theme	Signaling	114:122	arg1	Pathway					124:130	PI3K/AKT/NF-κB Signaling Pathway	99:130	PI3K/AKT/NF-κB Signaling Pathway	99:130	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.
35387258	4	106	theme	Methods	912:918	arg1	metabolomics					926:937	Methods Plant metabolomics	912:937	Methods Plant metabolomics	912:937	Methods Plant metabolomics was used to compare the chemical components of three species of rhubarb.
35387258	3	107	from	effect	743:748	arg1	VEI					753:755	VEI	753:755	VEI	753:755	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	13	108	theme	inflammation-induced	2448:2467	arg1	stress					2488:2493	inflammation-induced cellular oxidative stress	2448:2493	inflammation-induced cellular oxidative stress	2448:2493	All results indicated that the process of inflammation-induced cellular oxidative stress, which resulted in VEI, was obviously improved by three species of rhubarb.
35387258	3	109	theme	effect	743:748	arg1	species					703:709	the three species	693:709	the three species of rhubarb and their inhibitory effect on VEI	693:755	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	2	110	theme	blood	444:448	arg1	vessels					450:456	blood vessels	444:456	blood vessels	444:456	It has been reported that rhubarb can protect blood vessels by reducing inflammation and inhibiting vascular endothelial injury (VEI), but the effective components and mechanism of rhubarb inhibiting VEI are still unclear.
35387258	12	111	theme	rhubarb	2324:2330	arg1	species					2313:2319	three species	2307:2319	three species of rhubarb	2307:2330	In addition, three species of rhubarb could lessen the contents of ROS and NO in EA.hy926 cells induced by LPS.
35387258	3	112	from	VEI	753:755	arg1	species					703:709	the three species	693:709	the three species of rhubarb and their inhibitory effect on VEI	693:755	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	11	113	theme	proteins	2268:2275	arg1	inhibition					2254:2263	the inhibition	2250:2263	the inhibition of proteins	2250:2275	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	11	113	theme	proteins	2268:2275	arg1	pathway					2238:2244	the PI3K/AKT/NF-κB pathway	2219:2244	the PI3K/AKT/NF-κB pathway	2219:2244	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	0	114	theme	Species	20:26	arg1	Comparison					0:9	Comparison	0:9	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.	0:131	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.
35387258	14	115	theme	stress	3041:3046	arg1	reaction					3048:3055	the inflammation and oxidative stress reaction	3010:3055	the inflammation and oxidative stress reaction	3010:3055	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	9	116	theme	ion	1546:1548	arg1	mode					1550:1553	the positive ion mode	1533:1553	the positive ion mode	1533:1553	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	4	117	theme	species	992:998	arg1	components					972:981	the chemical components	959:981	the chemical components of three species of rhubarb	959:1009	Methods Plant metabolomics was used to compare the chemical components of three species of rhubarb.
35387258	1	118	attach	derived	188:194	arg2	medicine					179:186	a traditional Chinese medicine	157:186	a traditional Chinese medicine derived from three species	157:213	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	1	118	attach	derived	188:194	arg2	Rhubarb					148:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	1	118	attach	derived	188:194	arg1	species					207:213	three species	201:213	three species	201:213	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	0	119	theme	Rhubarb	31:37	arg1	Species					20:26	Three Species	14:26	Three Species of Rhubarb	14:37	Comparison of Three Species of Rhubarb in Inhibiting Vascular Endothelial Injury via Regulation of PI3K/AKT/NF-κB Signaling Pathway.
35387258	3	120	theme	inhibitory	886:895	arg1	effect					897:902	rhubarb's inhibitory effect	876:902	rhubarb's inhibitory effect on VEI	876:909	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	11	121	theme	cytokines	2113:2121	arg1	contents					2088:2095	the contents	2084:2095	the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α	2084:2144	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	9	122	theme	different	1562:1570	arg1	components					1581:1590	38 different chemical components	1559:1590	38 different chemical components in the negative ion mode	1559:1615	We identified 36 different chemical components in the positive ion mode and 38 different chemical components in the negative ion mode.
35387258	14	123	theme	Conclusion	2571:2580	arg1	tanguticum					2585:2594	Conclusion R. tanguticum	2571:2594	Conclusion R. tanguticum	2571:2594	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	4	124	theme	chemical	963:970	arg1	components					972:981	the chemical components	959:981	the chemical components of three species of rhubarb	959:1009	Methods Plant metabolomics was used to compare the chemical components of three species of rhubarb.
35387258	3	125	theme	chemical	668:675	arg1	compositions					677:688	chemical compositions	668:688	chemical compositions	668:688	This study aimed to compare the differences in chemical compositions of the three species of rhubarb and their inhibitory effect on VEI, so as to explain the material basis and select the dominant species to inhibit VEI, and to elucidate the mechanism of rhubarb's inhibitory effect on VEI.
35387258	14	126	theme	oxidative	3031:3039	arg1	stress					3041:3046	oxidative stress	3031:3046	oxidative stress	3031:3046	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	1	127	theme	traditional	325:335	arg1	effects					337:343	its traditional effects	321:343	its traditional effects of removing blood stasis and dredging the meridians	321:395	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	11	128	theme	protein	2208:2214	arg1	expressions					2161:2171	expressions	2161:2171	expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation	2161:2291	Three species of rhubarb alleviated the inflammatory response in LPS-induced EA.hy926 cells by reducing the contents of inflammatory cytokines IL-6, IL-1β, and TNF-α and decreasing expressions of PI3K, AKT, NF-κB p65, and STAT3 protein in the PI3K/AKT/NF-κB pathway and the inhibition of proteins phosphorylation.
35387258	10	129	theme	significant	1651:1661	arg1	differences					1663:1673	significant differences	1651:1673	significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO	1651:1818	Subsequently, the results showed significant differences in inhibiting VEI among the three species of rhubarb based on the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO and confirmed that R. tanguticum had the best inhibitory effect on VEI in the light of the comprehensive efficacy, compared with R. palmatum and R. officinale.
35387258	14	130	theme	inflammation	3014:3025	arg1	reaction					3048:3055	the inflammation and oxidative stress reaction	3010:3055	the inflammation and oxidative stress reaction	3010:3055	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35387258	1	131	used	used	228:231	arg2	medicine					179:186	a traditional Chinese medicine	157:186	a traditional Chinese medicine derived from three species	157:213	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	1	131	used	used	228:231	arg2	Rhubarb					148:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb	133:154	Background/Aim Rhubarb, a traditional Chinese medicine derived from three species, is commonly used in the prescriptions for promoting blood circulation and removing blood stasis based on its traditional effects of removing blood stasis and dredging the meridians.
35387258	8	132	theme	significant	1380:1390	arg1	differences					1392:1402	significant differences	1380:1402	significant differences in the distribution of chemical components among the three species of rhubarb	1380:1480	Results There were significant differences in the distribution of chemical components among the three species of rhubarb.
35387258	14	133	theme	ROS	2873:2875	arg1	contents					2806:2813	the contents	2802:2813	the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO	2802:2883	Conclusion R. tanguticum was more effective among three species of rhubarb, and it had been proved that gallic acid, gallic-acid-O-galloyl-glucoside, procyanidin B-2,3,3'-di-O-gallatein, and other potential components could reduce the contents of inflammatory factors (such as IL-1β, IL-6, and TNF-α), ROS, and NO by inhibiting the PI3K/AKT/NF-κB signaling pathway and protected the vascular endothelium and the blood vessels by improving the inflammation and oxidative stress reaction.
35320769	16	0	theme	carbon	2447:2452	arg1	bioenergetics					2461:2473	The finer-scale carbon uptake bioenergetics	2431:2473	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages	2431:2513	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	2	1	theme	full-scale	317:326	arg1	WWTPs					328:332	five full-scale WWTPs	312:332	five full-scale WWTPs	312:332	Fifteen samples were collected from five full-scale WWTPs.
35320769	7	2	dep	pathways	1232:1239	arg1	%					1252:1252	up to 49.2%	1242:1252	up to 49.2%	1242:1252	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	7	2	dep	pathways	1232:1239	arg1	%					1262:1262	61.0%	1258:1262	61.0%	1258:1262	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	6	3	theme	driving	758:764	arg1	%					783:783	up to 90.1%	773:783	up to 90.1%	773:783	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	3	theme	driving	758:764	arg1	force					766:770	the key driving force	750:770	the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs	750:918	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	3	theme	driving	758:764	arg1	force					725:729	proton motive force	711:729	proton motive force (PMF)	711:735	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	9	4	theme	major	1584:1588	arg1	differences					1610:1620	these major and community-level differences	1578:1620	differences	1610:1620	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	16	5	theme	GAOs	2486:2489	arg1	bioenergetics					2461:2473	The finer-scale carbon uptake bioenergetics	2431:2473	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages	2431:2513	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	9	6	theme	community-level	1594:1608	arg1	differences					1610:1620	these major and community-level differences	1578:1620	differences	1610:1620	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	11	7	from	dependency	1896:1905	arg1	operation					1922:1930	the reverse operation	1910:1930	the reverse operation of F1, F0-ATPase	1910:1947	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	6	8	theme	community	666:674	arg1	compositions					676:687	the distinct PAO/GAO community compositions	645:687	the distinct PAO/GAO community compositions in different samples	645:708	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	9	located	found	741:745	arg1	level					937:941	the community level	923:941	the community level	923:941	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	9	located	found	741:745	arg2	force					766:770	the key driving force	750:770	the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs	750:918	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	9	located	found	741:745	arg2	force					725:729	proton motive force	711:729	proton motive force (PMF)	711:735	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	9	located	found	741:745	arg2	PMF					732:734	PMF	732:734	PMF	732:734	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	10	theme	distinct	649:656	arg1	compositions					676:687	the distinct PAO/GAO community compositions	645:687	the distinct PAO/GAO community compositions in different samples	645:708	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	1	11	theme	PAOs	168:171	arg1	bioenergetics					151:163	the bioenergetics	147:163	the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources	147:273	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	6	12	theme	community	927:935	arg1	level					937:941	the community level	923:941	the community level	923:941	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	9	13	theme	level	1628:1632	arg1	micro-diversity					1645:1659	high level fine-scale micro-diversity	1623:1659	high level fine-scale micro-diversity in carbon uptake bioenergetics	1623:1690	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	16	14	from	interactions	2547:2558	arg1	assembly					2573:2580	community assembly	2563:2580	community assembly in complex environment	2563:2603	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	1	15	theme	GAOs	177:180	arg1	bioenergetics					151:163	the bioenergetics	147:163	the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources	147:273	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	6	16	theme	PMF	1024:1026	arg1	demand					1014:1019	a low demand	1008:1019	a low demand of PMF for acetate uptake	1008:1045	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	17	dep	understanding	969:981	arg1	have					1003:1006	have	1003:1006	have a low demand of PMF for acetate uptake	1003:1045	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	1	18	theme	full-scale	185:194	arg1	plants					217:222	full-scale wastewater treatment plants	185:222	full-scale wastewater treatment plants (WWTPs)	185:230	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	1	18	theme	full-scale	185:194	arg1	WWTPs					225:229	WWTPs	225:229	WWTPs	225:229	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	9	19	theme	carbon	1664:1669	arg1	bioenergetics					1678:1690	carbon uptake bioenergetics	1664:1690	carbon uptake bioenergetics	1664:1690	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	11	20	theme	F0-ATPase	1939:1947	arg1	operation					1922:1930	the reverse operation	1910:1930	the reverse operation of F1, F0-ATPase	1910:1947	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	16	21	theme	community	2563:2571	arg1	assembly					2573:2580	community assembly	2563:2580	community assembly in complex environment	2563:2603	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	7	22	theme	propionate	1077:1086	arg1	uptake					1056:1061	the uptake	1052:1061	the uptake of acetate or propionate	1052:1086	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	11	23	theme	higher	1889:1894	arg1	dependency					1896:1905	higher dependency	1889:1905	higher dependency on the reverse operation of F1, F0-ATPase than other PAOs	1889:1963	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	1	24	theme	first	135:139	arg1	time					141:144	the first time	131:144	the first time	131:144	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	0	25	from	bioenergetics	14:26	arg1	full-scale					48:57	full-scale	48:57	full-scale	48:57	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	0	26	theme	biological	68:77	arg1	systems					98:104	biological phosphorus removal systems	68:104	biological phosphorus removal systems	68:104	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	6	27	theme	acids	819:823	arg1	uptake					794:799	the uptake	790:799	the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs	790:918	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	28	dep	acids	819:823	arg1	VFAs					826:829	VFAs	826:829	VFAs	826:829	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	0	29	theme	removal	90:96	arg1	systems					98:104	biological phosphorus removal systems	68:104	biological phosphorus removal systems	68:104	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	6	30	theme	previous	960:967	arg1	understanding					969:981	the previous understanding	956:981	the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake	956:1045	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	7	31	theme	full-scale	1390:1399	arg1	systems					1401:1407	full-scale systems	1390:1407	full-scale systems	1390:1407	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	6	32	theme	volatile	804:811	arg1	acids					819:823	volatile fatty acids	804:823	volatile fatty acids (VFAs, acetate and propionate)	804:854	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	11	33	theme	other	1954:1958	arg1	PAOs					1960:1963	other PAOs	1954:1963	other PAOs	1954:1963	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	6	34	dep	VFAs	826:829	arg1	propionate					844:853	propionate	844:853	propionate	844:853	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	34	dep	VFAs	826:829	arg1	acetate					832:838	acetate	832:838	acetate	832:838	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	5	35	theme	gene	611:614	arg1	sequencing					625:634	rRNA gene amplicon sequencing	606:634	rRNA gene amplicon sequencing	606:634	Competibacter (0.08-5.39%), Defluviicoccus (0.05-5.34%), Micropruina (0.17-1.87%), etc., were shown by 16S rRNA gene amplicon sequencing.
35320769	8	36	theme	reductase	1527:1535	arg1	activities					1555:1564	fumarate reductase and F1, F0-ATPase activities	1518:1564	fumarate reductase and F1, F0-ATPase activities	1518:1564	However, different from VFAs, the uptake of glutamate and aspartate by both PAOs and GAOs commonly involved fumarate reductase and F1, F0-ATPase activities.
35320769	6	37	from	compositions	676:687	arg1	samples					702:708	different samples	692:708	different samples	692:708	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	3	38	theme	PAOs	361:364	arg1	Predominance					335:346	Predominance	335:346	Predominance	335:346	Predominance of different PAOs, i.e., Ca.
35320769	3	39	dep	Ca.	373:375	arg1	i.e.					367:370	i.e.	367:370	i.e.	367:370	Predominance of different PAOs, i.e., Ca.
35320769	0	40	theme	Carbon	0:5	arg1	bioenergetics					14:26	Carbon uptake bioenergetics	0:26	Carbon uptake bioenergetics of PAOs and GAOs in full-scale	0:57	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	4	41	dep	Microlunatus	433:444	arg1	phosphovorus					446:457	Microlunatus phosphovorus	433:457	Microlunatus phosphovorus (0.01-0.18%)	433:470	Accumulibacter (0.00-0.49%), Tetrasphaera (0.37-3.94%), Microlunatus phosphovorus (0.01-0.18%), etc., and GAOs, i.e., Ca.
35320769	7	42	from	difference	1344:1353	arg1	biogenetics					1375:1385	their VFA uptake biogenetics	1358:1385	their VFA uptake biogenetics	1358:1385	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	7	42	from	difference	1344:1353	arg1	systems					1401:1407	full-scale systems	1390:1407	full-scale systems	1390:1407	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	7	43	theme	VFA	1364:1366	arg1	biogenetics					1375:1385	their VFA uptake biogenetics	1358:1385	their VFA uptake biogenetics	1358:1385	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	6	44	theme	acids	866:870	arg1	uptake					794:799	the uptake	790:799	the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs	790:918	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	1	45	theme	different	250:258	arg1	sources					267:273	different carbon sources	250:273	different carbon sources	250:273	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	11	46	theme	low	1984:1986	arg1	involvement					1988:1998	the low involvement	1980:1998	the low involvement of glyoxylate shunt pathway	1980:2026	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	16	47	theme	different	2496:2504	arg1	lineages					2506:2513	different lineages	2496:2513	different lineages	2496:2513	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	0	48	theme	PAOs	31:34	arg1	bioenergetics					14:26	Carbon uptake bioenergetics	0:26	Carbon uptake bioenergetics of PAOs and GAOs in full-scale	0:57	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	1	49	theme	sources	267:273	arg1	uptake					240:245	the uptake	236:245	the uptake of different carbon sources	236:273	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	14	50	theme	stain	2280:2284	arg1	NM-1					2286:2289	Microlunatus phosphovorus stain NM-1	2254:2289	Microlunatus phosphovorus stain NM-1	2254:2289	This feature was testified using a pure culture of Microlunatus phosphovorus stain NM-1.
35320769	9	51	located	observed	1696:1703	arg2	micro-diversity					1645:1659	high level fine-scale micro-diversity	1623:1659	high level fine-scale micro-diversity in carbon uptake bioenergetics	1623:1690	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	9	51	located	observed	1696:1703	arg1	GAO					1720:1722	GAO	1720:1722	GAO	1720:1722	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	9	51	located	observed	1696:1703	arg1	PAO					1712:1714	PAO	1712:1714	PAO	1712:1714	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	9	52	theme	different	1791:1799	arg1	pathways					1801:1808	different pathways	1791:1808	different pathways	1791:1808	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	0	53	theme	GAOs	40:43	arg1	bioenergetics					14:26	Carbon uptake bioenergetics	0:26	Carbon uptake bioenergetics of PAOs and GAOs in full-scale	0:57	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	14	54	theme	Microlunatus	2254:2265	arg1	NM-1					2286:2289	Microlunatus phosphovorus stain NM-1	2254:2289	Microlunatus phosphovorus stain NM-1	2254:2289	This feature was testified using a pure culture of Microlunatus phosphovorus stain NM-1.
35320769	1	55	theme	wastewater	196:205	arg1	plants					217:222	full-scale wastewater treatment plants	185:222	full-scale wastewater treatment plants (WWTPs)	185:230	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	1	55	theme	wastewater	196:205	arg1	WWTPs					225:229	WWTPs	225:229	WWTPs	225:229	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	6	56	theme	proton	711:716	arg1	force					766:770	the key driving force	750:770	the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs	750:918	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	56	theme	proton	711:716	arg1	force					725:729	proton motive force	711:729	proton motive force (PMF)	711:735	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	56	theme	proton	711:716	arg1	PMF					732:734	PMF	732:734	PMF	732:734	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	11	57	theme	pathway	2020:2026	arg1	involvement					1988:1998	the low involvement	1980:1998	the low involvement of glyoxylate shunt pathway	1980:2026	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	9	58	theme	power	1823:1827	arg1	generation					1829:1838	power generation	1823:1838	power generation	1823:1838	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	8	59	from	VFAs	1434:1437	arg1	different					1419:1427	different	1419:1427	different	1419:1427	However, different from VFAs, the uptake of glutamate and aspartate by both PAOs and GAOs commonly involved fumarate reductase and F1, F0-ATPase activities.
35320769	16	60	from	assembly	2573:2580	arg1	environment					2593:2603	complex environment	2585:2603	complex environment	2585:2603	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	8	61	theme	F0-ATPase	1545:1553	arg1	activities					1555:1564	fumarate reductase and F1, F0-ATPase activities	1518:1564	fumarate reductase and F1, F0-ATPase activities	1518:1564	However, different from VFAs, the uptake of glutamate and aspartate by both PAOs and GAOs commonly involved fumarate reductase and F1, F0-ATPase activities.
35320769	7	62	theme	intensive	1196:1204	arg1	involvements					1206:1217	intensive involvements	1196:1217	intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively)	1196:1277	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	16	63	theme	finer-scale	2435:2445	arg1	bioenergetics					2461:2473	The finer-scale carbon uptake bioenergetics	2431:2473	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages	2431:2513	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	6	64	theme	different	692:700	arg1	samples					702:708	different samples	692:708	different samples	692:708	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	15	65	theme	major	2296:2300	arg1	difference					2302:2311	The major difference	2292:2311	The major difference between PAOs and GAOs	2292:2333	The major difference between PAOs and GAOs highlights the potential to selectively suppress GAOs for community regulation in EBPR systems.
35320769	4	66	dep	Tetrasphaera	406:417	arg1	Ca.					495:497	Tetrasphaera (0.37-3.94%), Microlunatus phosphovorus (0.01-0.18%), etc., and GAOs, i.e., Ca.	406:497	Ca.	495:497	Accumulibacter (0.00-0.49%), Tetrasphaera (0.37-3.94%), Microlunatus phosphovorus (0.01-0.18%), etc., and GAOs, i.e., Ca.
35320769	6	67	theme	key	754:756	arg1	%					783:783	up to 90.1%	773:783	up to 90.1%	773:783	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	67	theme	key	754:756	arg1	force					766:770	the key driving force	750:770	the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs	750:918	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	67	theme	key	754:756	arg1	force					725:729	proton motive force	711:729	proton motive force (PMF)	711:735	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	7	68	theme	PMF	1171:1173	arg1	generation					1175:1184	PMF generation	1171:1184	PMF generation	1171:1184	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	15	69	theme	community	2393:2401	arg1	regulation					2403:2412	community regulation	2393:2412	community regulation	2393:2412	The major difference between PAOs and GAOs highlights the potential to selectively suppress GAOs for community regulation in EBPR systems.
35320769	16	70	theme	complex	2585:2591	arg1	environment					2593:2603	complex environment	2585:2603	complex environment	2585:2603	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	6	71	theme	acetate	1032:1038	arg1	uptake					1040:1045	acetate uptake	1032:1045	acetate uptake	1032:1045	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	72	theme	PAO/GAO	658:664	arg1	compositions					676:687	the distinct PAO/GAO community compositions	645:687	the distinct PAO/GAO community compositions in different samples	645:708	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	16	73	from	lineages	2506:2513	arg1	bioenergetics					2461:2473	The finer-scale carbon uptake bioenergetics	2431:2473	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages	2431:2513	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	7	74	theme	community-level	1328:1342	arg1	difference					1344:1353	a major and community-level difference	1316:1353	a major and community-level difference in their VFA uptake biogenetics in full-scale systems	1316:1407	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	9	75	theme	high	1623:1626	arg1	micro-diversity					1645:1659	high level fine-scale micro-diversity	1623:1659	high level fine-scale micro-diversity in carbon uptake bioenergetics	1623:1690	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	9	76	dep	PAO	1712:1714	arg1	lineages					1724:1731	lineages	1724:1731	lineages	1724:1731	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	7	77	theme	major	1318:1322	arg1	difference					1344:1353	a major and community-level difference	1316:1353	a major and community-level difference in their VFA uptake biogenetics in full-scale systems	1316:1407	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	6	78	dep	90.1	779:782	arg1	to					776:777	to	776:777	to	776:777	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	9	79	theme	fine-scale	1634:1643	arg1	micro-diversity					1645:1659	high level fine-scale micro-diversity	1623:1659	high level fine-scale micro-diversity in carbon uptake bioenergetics	1623:1690	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	11	80	theme	glyoxylate	2003:2012	arg1	pathway					2020:2026	glyoxylate shunt pathway	2003:2026	glyoxylate shunt pathway	2003:2026	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	9	81	from	micro-diversity	1645:1659	arg1	bioenergetics					1678:1690	carbon uptake bioenergetics	1664:1690	carbon uptake bioenergetics	1664:1690	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	1	82	from	plants	217:222	arg1	bioenergetics					151:163	the bioenergetics	147:163	the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources	147:273	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	12	83	dep	Ca.	2065:2067	arg1	Tetrasphaera					2036:2047	Tetrasphaera	2036:2047	Tetrasphaera	2036:2047	Unlike Tetrasphaera, but similar to Ca.
35320769	6	84	theme	low	1010:1012	arg1	demand					1014:1019	a low demand	1008:1019	a low demand of PMF for acetate uptake	1008:1045	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	9	85	theme	uptake	1671:1676	arg1	bioenergetics					1678:1690	carbon uptake bioenergetics	1664:1690	carbon uptake bioenergetics	1664:1690	Apart from these major and community-level differences, high level fine-scale micro-diversity in carbon uptake bioenergetics was observed within PAO and GAO lineages, probably resulting from their versatilities in employing different pathways for reducing power generation.
35320769	8	86	theme	glutamate	1454:1462	arg1	uptake					1444:1449	the uptake	1440:1449	the uptake of glutamate and aspartate by both PAOs and GAOs	1440:1498	However, different from VFAs, the uptake of glutamate and aspartate by both PAOs and GAOs commonly involved fumarate reductase and F1, F0-ATPase activities.
35320769	7	87	theme	fumarate	1139:1146	arg1	reductase					1148:1156	fumarate reductase	1139:1156	fumarate reductase (< 5.3%)	1139:1165	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	7	87	theme	fumarate	1139:1146	arg1	%					1164:1164	< 5.3%	1159:1164	< 5.3%	1159:1164	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	11	88	theme	shunt	2014:2018	arg1	pathway					2020:2026	glyoxylate shunt pathway	2003:2026	glyoxylate shunt pathway	2003:2026	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	5	89	theme	amplicon	616:623	arg1	sequencing					625:634	rRNA gene amplicon sequencing	606:634	rRNA gene amplicon sequencing	606:634	Competibacter (0.08-5.39%), Defluviicoccus (0.05-5.34%), Micropruina (0.17-1.87%), etc., were shown by 16S rRNA gene amplicon sequencing.
35320769	8	90	theme	aspartate	1468:1476	arg1	uptake					1444:1449	the uptake	1440:1449	the uptake of glutamate and aspartate by both PAOs and GAOs	1440:1498	However, different from VFAs, the uptake of glutamate and aspartate by both PAOs and GAOs commonly involved fumarate reductase and F1, F0-ATPase activities.
35320769	7	91	theme	F0-	1115:1117	arg1	ATPase					1119:1124	F0- ATPase	1115:1124	F0- ATPase (< 11.7%)	1115:1134	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	7	91	theme	F0-	1115:1117	arg1	%					1133:1133	< 11.7%	1127:1133	< 11.7%	1127:1133	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	0	92	theme	phosphorus	79:88	arg1	systems					98:104	biological phosphorus removal systems	68:104	biological phosphorus removal systems	68:104	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	1	93	from	bioenergetics	151:163	arg1	plants					217:222	full-scale wastewater treatment plants	185:222	full-scale wastewater treatment plants (WWTPs)	185:230	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	1	93	from	bioenergetics	151:163	arg1	WWTPs					225:229	WWTPs	225:229	WWTPs	225:229	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	15	94	theme	EBPR	2417:2420	arg1	systems					2422:2428	EBPR systems	2417:2428	EBPR systems	2417:2428	The major difference between PAOs and GAOs highlights the potential to selectively suppress GAOs for community regulation in EBPR systems.
35320769	13	95	theme	proton/glutamate-aspartate	2151:2176	arg1	symporter					2178:2186	the proton/glutamate-aspartate symporter	2147:2186	the proton/glutamate-aspartate symporter driven by PMF	2147:2200	Accumulibacter, Microlunatus phosphovorus took up glutamate and aspartate via the proton/glutamate-aspartate symporter driven by PMF.
35320769	6	96	theme	fatty	813:817	arg1	acids					819:823	volatile fatty acids	804:823	volatile fatty acids (VFAs, acetate and propionate)	804:854	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	5	97	theme	rRNA	606:609	arg1	sequencing					625:634	rRNA gene amplicon sequencing	606:634	rRNA gene amplicon sequencing	606:634	Competibacter (0.08-5.39%), Defluviicoccus (0.05-5.34%), Micropruina (0.17-1.87%), etc., were shown by 16S rRNA gene amplicon sequencing.
35320769	1	98	theme	treatment	207:215	arg1	plants					217:222	full-scale wastewater treatment plants	185:222	full-scale wastewater treatment plants (WWTPs)	185:230	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	1	98	theme	treatment	207:215	arg1	WWTPs					225:229	WWTPs	225:229	WWTPs	225:229	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	0	99	theme	uptake	7:12	arg1	bioenergetics					14:26	Carbon uptake bioenergetics	0:26	Carbon uptake bioenergetics of PAOs and GAOs in full-scale	0:57	Carbon uptake bioenergetics of PAOs and GAOs in full-scale enhanced biological phosphorus removal systems.
35320769	8	100	theme	fumarate	1518:1525	arg1	reductase					1527:1535	fumarate reductase	1518:1535	fumarate reductase	1518:1535	However, different from VFAs, the uptake of glutamate and aspartate by both PAOs and GAOs commonly involved fumarate reductase and F1, F0-ATPase activities.
35320769	16	101	theme	PAOs	2478:2481	arg1	bioenergetics					2461:2473	The finer-scale carbon uptake bioenergetics	2431:2473	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages	2431:2513	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
35320769	14	102	theme	pure	2238:2241	arg1	culture					2243:2249	a pure culture	2236:2249	a pure culture of Microlunatus phosphovorus stain NM-1	2236:2289	This feature was testified using a pure culture of Microlunatus phosphovorus stain NM-1.
35320769	6	103	dep	acids	866:870	arg1	aspartate					887:895	aspartate	887:895	aspartate	887:895	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	103	dep	acids	866:870	arg1	glutamate					873:881	glutamate	873:881	glutamate	873:881	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	103	dep	acids	866:870	arg1	acids					866:870	amino acids	860:870	amino acids (glutamate and aspartate)	860:896	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	7	104	theme	uptake	1368:1373	arg1	biogenetics					1375:1385	their VFA uptake biogenetics	1358:1385	their VFA uptake biogenetics	1358:1385	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	6	105	theme	amino	860:864	arg1	aspartate					887:895	aspartate	887:895	aspartate	887:895	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	105	theme	amino	860:864	arg1	glutamate					873:881	glutamate	873:881	glutamate	873:881	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	105	theme	amino	860:864	arg1	acids					866:870	amino acids	860:870	amino acids (glutamate and aspartate)	860:896	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	3	106	theme	different	351:359	arg1	PAOs					361:364	different PAOs	351:364	different PAOs	351:364	Predominance of different PAOs, i.e., Ca.
35320769	1	107	theme	carbon	260:265	arg1	sources					267:273	different carbon sources	250:273	different carbon sources	250:273	This work analyzed, for the first time, the bioenergetics of PAOs and GAOs in full-scale wastewater treatment plants (WWTPs) for the uptake of different carbon sources.
35320769	14	108	theme	NM-1	2286:2289	arg1	culture					2243:2249	a pure culture	2236:2249	a pure culture of Microlunatus phosphovorus stain NM-1	2236:2289	This feature was testified using a pure culture of Microlunatus phosphovorus stain NM-1.
35320769	7	109	theme	acetate	1066:1072	arg1	uptake					1056:1061	the uptake	1052:1061	the uptake of acetate or propionate	1052:1086	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	14	110	theme	phosphovorus	2267:2278	arg1	NM-1					2286:2289	Microlunatus phosphovorus stain NM-1	2254:2289	Microlunatus phosphovorus stain NM-1	2254:2289	This feature was testified using a pure culture of Microlunatus phosphovorus stain NM-1.
35320769	11	111	theme	reverse	1914:1920	arg1	operation					1922:1930	the reverse operation	1910:1930	the reverse operation of F1, F0-ATPase	1910:1947	Accumulibacter and Halomonas seemed to show higher dependency on the reverse operation of F1, F0-ATPase than other PAOs, likely due to the low involvement of glyoxylate shunt pathway.
35320769	6	112	contain	have	1003:1006	arg2	demand					1014:1019	a low demand	1008:1019	a low demand of PMF for acetate uptake	1008:1045	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	112	contain	have	1003:1006	arg1	Defluviicoccus					988:1001	Defluviicoccus	988:1001	Defluviicoccus	988:1001	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	113	theme	motive	718:723	arg1	force					766:770	the key driving force	750:770	the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs	750:918	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	113	theme	motive	718:723	arg1	force					725:729	proton motive force	711:729	proton motive force (PMF)	711:735	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	6	113	theme	motive	718:723	arg1	PMF					732:734	PMF	732:734	PMF	732:734	Despite the distinct PAO/GAO community compositions in different samples, proton motive force (PMF) was found as the key driving force (up to 90.1%) for the uptake of volatile fatty acids (VFAs, acetate and propionate) and amino acids (glutamate and aspartate) by both GAOs and PAOs at the community level, contrasting the previous understanding that Defluviicoccus have a low demand of PMF for acetate uptake.
35320769	15	114	from	GAOs	2384:2387	arg1	systems					2422:2428	EBPR systems	2417:2428	EBPR systems	2417:2428	The major difference between PAOs and GAOs highlights the potential to selectively suppress GAOs for community regulation in EBPR systems.
35320769	7	115	dep	49.2	1248:1251	arg1	to					1245:1246	to	1245:1246	to	1245:1246	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	7	116	theme	pathways	1232:1239	arg1	involvements					1206:1217	intensive involvements	1196:1217	intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively)	1196:1277	For the uptake of acetate or propionate, PAOs rarely activated F1, F0- ATPase (< 11.7%) or fumarate reductase (< 5.3%) for PMF generation; whereas, intensive involvements of these two pathways (up to 49.2% and 61.0%, respectively) were observed for GAOs, highlighting a major and community-level difference in their VFA uptake biogenetics in full-scale systems.
35320769	16	117	theme	uptake	2454:2459	arg1	bioenergetics					2461:2473	The finer-scale carbon uptake bioenergetics	2431:2473	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages	2431:2513	The finer-scale carbon uptake bioenergetics of PAOs or GAOs from different lineages benefits in understanding their interactions in community assembly in complex environment.
36087619	7	0	theme	inflammatory	1074:1085	arg1	cells					1087:1091	inflammatory cells	1074:1091	inflammatory cells	1074:1091	Instead, ingested nTiO2 yielded shorter colon, aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
36087619	5	1	from	phenotypes	646:655	arg1	microbiome					735:744	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	microbiome	735:744	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	1	from	phenotypes	646:655	arg1	sequencing					751:760	16S sequencing	747:760	16S sequencing	747:760	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	1	from	phenotypes	646:655	arg1	sequencing					715:724	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	sequencing	715:724	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	2	2	theme	potential	330:338	arg1	toxicities					340:349	their potential toxicities	324:349	their potential toxicities in the dysfunctional intestine	324:380	Despite their increased oral exposure, their potential toxicities in the dysfunctional intestine are unclear.
36087619	11	3	theme	metallic	1527:1534	arg1	nanoparticles					1536:1548	metallic nanoparticles	1527:1548	metallic nanoparticles in food products	1527:1565	The results provide valuable information for evaluation of utilizing metallic nanoparticles in food products for the vulnerable population.
36087619	1	4	theme	anti-microbial	209:222	arg1	properties					224:233	anti-microbial properties	209:233	anti-microbial properties	209:233	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	6	5	theme	mice	846:849	arg1	hallmarks					825:833	phenotypic hallmarks	814:833	phenotypic hallmarks of colitic mice	814:849	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	7	6	theme	cells	1087:1091	arg1	hyperplasia					1035:1045	epithelial hyperplasia	1024:1045	epithelial hyperplasia	1024:1045	Instead, ingested nTiO2 yielded shorter colon, aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
36087619	7	6	theme	cells	1087:1091	arg1	infiltration					1058:1069	deeper infiltration	1051:1069	deeper infiltration	1051:1069	Instead, ingested nTiO2 yielded shorter colon, aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
36087619	8	7	theme	gut	1139:1141	arg1	composition					1154:1164	the gut microbiota composition	1135:1164	the gut microbiota composition	1135:1164	Both nanoparticles significantly changed the gut microbiota composition, resulting in loss of diversity and increase of potential pathobionts.
36087619	6	8	theme	death	940:944	arg1	processes					912:920	processes	912:920	processes of apoptotic cell death and RNA metabolism	912:963	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	6	9	theme	colitic	838:844	arg1	mice					846:849	colitic mice	838:849	colitic mice	838:849	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	3	10	theme	acute	507:511	arg1	colitis					524:530	chemical-induced acute ulcerative colitis	490:530	chemical-induced acute ulcerative colitis	490:530	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	6	11	theme	cell	935:938	arg1	death					940:944	apoptotic cell death	925:944	apoptotic cell death	925:944	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	6	12	theme	apoptotic	925:933	arg1	death					940:944	apoptotic cell death	925:944	apoptotic cell death	925:944	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	3	13	theme	ulcerative	513:522	arg1	colitis					524:530	chemical-induced acute ulcerative colitis	490:530	chemical-induced acute ulcerative colitis	490:530	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	1	14	theme	various	238:244	arg1	products					251:258	various food products	238:258	various food products	238:258	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	5	15	theme	colonic	688:694	arg1	sequencing					751:760	16S sequencing	747:760	16S sequencing	747:760	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	15	theme	colonic	688:694	arg1	sequencing					715:724	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	sequencing	715:724	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	9	16	theme	Akkermansia	1288:1298	arg1	muciniphila					1300:1310	Akkermansia muciniphila	1288:1310	Akkermansia muciniphila	1288:1310	They also increased colonic mucus and abundance of Akkermansia muciniphila.
36087619	8	17	theme	microbiota	1143:1152	arg1	composition					1154:1164	the gut microbiota composition	1135:1164	the gut microbiota composition	1135:1164	Both nanoparticles significantly changed the gut microbiota composition, resulting in loss of diversity and increase of potential pathobionts.
36087619	5	18	from	histology	658:666	arg1	microbiome					735:744	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	microbiome	735:744	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	18	from	histology	658:666	arg1	sequencing					751:760	16S sequencing	747:760	16S sequencing	747:760	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	18	from	histology	658:666	arg1	sequencing					715:724	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	sequencing	715:724	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	8	19	theme	pathobionts	1224:1234	arg1	increase					1202:1209	increase	1202:1209	increase of potential pathobionts	1202:1234	Both nanoparticles significantly changed the gut microbiota composition, resulting in loss of diversity and increase of potential pathobionts.
36087619	8	19	theme	pathobionts	1224:1234	arg1	loss					1180:1183	loss	1180:1183	loss of diversity	1180:1196	Both nanoparticles significantly changed the gut microbiota composition, resulting in loss of diversity and increase of potential pathobionts.
36087619	4	20	theme	consecutive	603:613	arg1	days					615:618	10 consecutive days	600:618	10 consecutive days	600:618	Mice (eight/group) were exposed to nAg or nTiO2 by oral gavage for 10 consecutive days.
36087619	0	21	theme	mouse	90:94	arg1	model					96:100	a mouse model	88:100	a mouse model of ulcerative colitis	88:122	Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and microbiota in a mouse model of ulcerative colitis.
36087619	6	22	theme	phenotypic	814:823	arg1	hallmarks					825:833	phenotypic hallmarks	814:833	phenotypic hallmarks of colitic mice	814:849	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	7	23	theme	deeper	1051:1056	arg1	infiltration					1058:1069	deeper infiltration	1051:1069	deeper infiltration	1051:1069	Instead, ingested nTiO2 yielded shorter colon, aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
36087619	11	24	theme	vulnerable	1575:1584	arg1	population					1586:1595	the vulnerable population	1571:1595	the vulnerable population	1571:1595	The results provide valuable information for evaluation of utilizing metallic nanoparticles in food products for the vulnerable population.
36087619	3	25	theme	chemical-induced	490:505	arg1	colitis					524:530	chemical-induced acute ulcerative colitis	490:530	chemical-induced acute ulcerative colitis	490:530	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	1	26	theme	titanium	142:149	arg1	dioxide					151:157	Silver (nAg) and titanium dioxide	125:157	dioxide	151:157	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	5	27	theme	16S	747:749	arg1	sequencing					751:760	16S sequencing	747:760	16S sequencing	747:760	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	27	theme	16S	747:749	arg1	sequencing					715:724	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	sequencing	715:724	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	7	28	theme	epithelial	1024:1033	arg1	hyperplasia					1035:1045	epithelial hyperplasia	1024:1045	epithelial hyperplasia	1024:1045	Instead, ingested nTiO2 yielded shorter colon, aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
36087619	2	29	theme	dysfunctional	358:370	arg1	intestine					372:380	the dysfunctional intestine	354:380	the dysfunctional intestine	354:380	Despite their increased oral exposure, their potential toxicities in the dysfunctional intestine are unclear.
36087619	0	30	theme	Oral	0:3	arg1	exposure					5:12	Oral exposure	0:12	Oral exposure to Ag or TiO2	0:26	Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and microbiota in a mouse model of ulcerative colitis.
36087619	1	31	theme	food	246:249	arg1	products					251:258	various food products	238:258	various food products	238:258	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	10	32	theme	inflammation	1444:1455	arg1	context					1427:1433	the context	1423:1433	the context of colon inflammation	1423:1455	Overall, nAg and nTiO2 induce dissimilar immunotoxicological changes at the molecular and microbiome level in the context of colon inflammation.
36087619	10	33	theme	microbiome	1403:1412	arg1	level					1414:1418	the molecular and microbiome level	1385:1418	level	1414:1418	Overall, nAg and nTiO2 induce dissimilar immunotoxicological changes at the molecular and microbiome level in the context of colon inflammation.
36087619	11	34	from	nanoparticles	1536:1548	arg1	products					1558:1565	food products	1553:1565	food products	1553:1565	The results provide valuable information for evaluation of utilizing metallic nanoparticles in food products for the vulnerable population.
36087619	8	35	theme	potential	1214:1222	arg1	pathobionts					1224:1234	potential pathobionts	1214:1234	potential pathobionts	1214:1234	Both nanoparticles significantly changed the gut microbiota composition, resulting in loss of diversity and increase of potential pathobionts.
36087619	1	36	theme	products	251:258	arg1	flavour					198:204	flavour	198:204	flavour	198:204	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	1	36	theme	products	251:258	arg1	properties					224:233	anti-microbial properties	209:233	anti-microbial properties	209:233	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	1	36	theme	products	251:258	arg1	texture					189:195	texture	189:195	texture	189:195	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	0	37	theme	ulcerative	105:114	arg1	colitis					116:122	ulcerative colitis	105:122	ulcerative colitis	105:122	Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and microbiota in a mouse model of ulcerative colitis.
36087619	2	38	from	toxicities	340:349	arg1	intestine					372:380	the dysfunctional intestine	354:380	the dysfunctional intestine	354:380	Despite their increased oral exposure, their potential toxicities in the dysfunctional intestine are unclear.
36087619	3	39	theme	colitis	524:530	arg1	model					481:485	a mouse model	473:485	a mouse model of chemical-induced acute ulcerative colitis	473:530	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	10	40	theme	molecular	1389:1397	arg1	level					1414:1418	the molecular and microbiome level	1385:1418	level	1414:1418	Overall, nAg and nTiO2 induce dissimilar immunotoxicological changes at the molecular and microbiome level in the context of colon inflammation.
36087619	3	41	theme	nTiO2	432:436	arg1	effects					405:411	the effects	401:411	the effects of ingested nAg or nTiO2 on inflamed colon	401:454	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	10	42	theme	dissimilar	1343:1352	arg1	changes					1374:1380	dissimilar immunotoxicological changes	1343:1380	dissimilar immunotoxicological changes	1343:1380	Overall, nAg and nTiO2 induce dissimilar immunotoxicological changes at the molecular and microbiome level in the context of colon inflammation.
36087619	1	43	theme	Silver	125:130	arg1	nAg					133:135	Silver (nAg) and titanium dioxide	125:157	nAg	133:135	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	1	43	theme	Silver	125:130	arg1	nTiO2					160:164	nTiO2	160:164	nTiO2	160:164	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	1	44	theme	packaging	264:272	arg1	materials					274:282	packaging materials	264:282	packaging materials	264:282	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	7	45	theme	shorter	998:1004	arg1	colon					1006:1010	shorter colon	998:1010	shorter colon	998:1010	Instead, ingested nTiO2 yielded shorter colon, aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
36087619	6	46	theme	minor	797:801	arg1	changes					803:809	only minor changes	792:809	only minor changes	792:809	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	11	47	theme	food	1553:1556	arg1	products					1558:1565	food products	1553:1565	food products	1553:1565	The results provide valuable information for evaluation of utilizing metallic nanoparticles in food products for the vulnerable population.
36087619	11	48	theme	valuable	1478:1485	arg1	information					1487:1497	valuable information	1478:1497	valuable information for evaluation of utilizing metallic nanoparticles in food products for the vulnerable population	1478:1595	The results provide valuable information for evaluation of utilizing metallic nanoparticles in food products for the vulnerable population.
36087619	3	49	theme	nAg	425:427	arg1	effects					405:411	the effects	401:411	the effects of ingested nAg or nTiO2 on inflamed colon	401:454	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	5	50	theme	gut	731:733	arg1	microbiome					735:744	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	microbiome	735:744	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	1	51	theme	materials	274:282	arg1	flavour					198:204	flavour	198:204	flavour	198:204	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	1	51	theme	materials	274:282	arg1	properties					224:233	anti-microbial properties	209:233	anti-microbial properties	209:233	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	1	51	theme	materials	274:282	arg1	texture					189:195	texture	189:195	texture	189:195	Silver (nAg) and titanium dioxide (nTiO2) nanoparticles improve texture, flavour or anti-microbial properties of various food products and packaging materials.
36087619	0	52	theme	colitis	116:122	arg1	model					96:100	a mouse model	88:100	a mouse model of ulcerative colitis	88:122	Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and microbiota in a mouse model of ulcerative colitis.
36087619	5	53	from	alterations	673:683	arg1	microbiome					735:744	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	microbiome	735:744	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	53	from	alterations	673:683	arg1	sequencing					751:760	16S sequencing	747:760	16S sequencing	747:760	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	53	from	alterations	673:683	arg1	sequencing					715:724	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	sequencing	715:724	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	54	theme	transcriptome	696:708	arg1	sequencing					751:760	16S sequencing	747:760	16S sequencing	747:760	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	54	theme	transcriptome	696:708	arg1	sequencing					715:724	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	sequencing	715:724	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	0	55	theme	perturbed	42:50	arg1	transcriptome					56:68	perturbed gut transcriptome	42:68	perturbed gut transcriptome	42:68	Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and microbiota in a mouse model of ulcerative colitis.
36087619	6	56	theme	gene	886:889	arg1	expression					891:900	gene expression	886:900	gene expression enriching processes of apoptotic cell death and RNA metabolism	886:963	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	5	57	theme	RNA	711:713	arg1	sequencing					751:760	16S sequencing	747:760	16S sequencing	747:760	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	5	57	theme	RNA	711:713	arg1	sequencing					715:724	colonic transcriptome (RNA sequencing) and gut microbiome	688:744	sequencing	715:724	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	9	58	theme	muciniphila	1300:1310	arg1	abundance					1275:1283	colonic mucus and abundance	1257:1283	abundance	1275:1283	They also increased colonic mucus and abundance of Akkermansia muciniphila.
36087619	9	58	theme	muciniphila	1300:1310	arg1	mucus					1265:1269	colonic mucus and abundance	1257:1283	mucus	1265:1269	They also increased colonic mucus and abundance of Akkermansia muciniphila.
36087619	8	59	theme	diversity	1188:1196	arg1	increase					1202:1209	increase	1202:1209	increase of potential pathobionts	1202:1234	Both nanoparticles significantly changed the gut microbiota composition, resulting in loss of diversity and increase of potential pathobionts.
36087619	8	59	theme	diversity	1188:1196	arg1	loss					1180:1183	loss	1180:1183	loss of diversity	1180:1196	Both nanoparticles significantly changed the gut microbiota composition, resulting in loss of diversity and increase of potential pathobionts.
36087619	6	60	theme	extensive	863:871	arg1	responses					873:881	extensive responses	863:881	extensive responses	863:881	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	5	61	theme	disease	638:644	arg1	phenotypes					646:655	disease phenotypes	638:655	disease phenotypes	638:655	We characterized disease phenotypes, histology, and alterations in colonic transcriptome (RNA sequencing) and gut microbiome (16S sequencing).
36087619	2	62	theme	oral	309:312	arg1	exposure					314:321	their increased oral exposure	293:321	their increased oral exposure	293:321	Despite their increased oral exposure, their potential toxicities in the dysfunctional intestine are unclear.
36087619	7	63	theme	ingested	975:982	arg1	nTiO2					984:988	ingested nTiO2	975:988	ingested nTiO2	975:988	Instead, ingested nTiO2 yielded shorter colon, aggravated epithelial hyperplasia and deeper infiltration of inflammatory cells.
36087619	10	64	theme	immunotoxicological	1354:1372	arg1	changes					1374:1380	dissimilar immunotoxicological changes	1343:1380	dissimilar immunotoxicological changes	1343:1380	Overall, nAg and nTiO2 induce dissimilar immunotoxicological changes at the molecular and microbiome level in the context of colon inflammation.
36087619	2	65	theme	increased	299:307	arg1	exposure					314:321	their increased oral exposure	293:321	their increased oral exposure	293:321	Despite their increased oral exposure, their potential toxicities in the dysfunctional intestine are unclear.
36087619	3	66	theme	inflamed	441:448	arg1	colon					450:454	inflamed colon	441:454	inflamed colon	441:454	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	6	67	theme	Oral	764:767	arg1	exposure					769:776	Oral exposure	764:776	Oral exposure to nAg	764:783	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	3	68	from	effects	405:411	arg1	colon					450:454	inflamed colon	441:454	inflamed colon	441:454	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	3	69	theme	mouse	475:479	arg1	model					481:485	a mouse model	473:485	a mouse model of chemical-induced acute ulcerative colitis	473:530	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	3	70	theme	ingested	416:423	arg1	nAg					425:427	nAg	425:427	nAg	425:427	Here, the effects of ingested nAg or nTiO2 on inflamed colon were revealed in a mouse model of chemical-induced acute ulcerative colitis.
36087619	6	71	theme	metabolism	954:963	arg1	processes					912:920	processes	912:920	processes of apoptotic cell death and RNA metabolism	912:963	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36087619	0	72	theme	gut	52:54	arg1	transcriptome					56:68	perturbed gut transcriptome	42:68	perturbed gut transcriptome	42:68	Oral exposure to Ag or TiO2 nanoparticles perturbed gut transcriptome and microbiota in a mouse model of ulcerative colitis.
36087619	9	73	theme	colonic	1257:1263	arg1	mucus					1265:1269	colonic mucus and abundance	1257:1283	mucus	1265:1269	They also increased colonic mucus and abundance of Akkermansia muciniphila.
36087619	4	74	theme	oral	584:587	arg1	gavage					589:594	oral gavage	584:594	oral gavage for 10 consecutive days	584:618	Mice (eight/group) were exposed to nAg or nTiO2 by oral gavage for 10 consecutive days.
36087619	10	75	theme	colon	1438:1442	arg1	inflammation					1444:1455	colon inflammation	1438:1455	colon inflammation	1438:1455	Overall, nAg and nTiO2 induce dissimilar immunotoxicological changes at the molecular and microbiome level in the context of colon inflammation.
36087619	6	76	theme	RNA	950:952	arg1	metabolism					954:963	RNA metabolism	950:963	RNA metabolism	950:963	Oral exposure to nAg caused only minor changes in phenotypic hallmarks of colitic mice but induced extensive responses in gene expression enriching processes of apoptotic cell death and RNA metabolism.
36453715	0	0	theme	intestinal	101:110	arg1	microbiota					112:121	intestinal microbiota	101:121	intestinal microbiota	101:121	Tieguanyin extracts ameliorated DSS-induced mouse colitis by suppressing inflammation and regulating intestinal microbiota.
36453715	3	1	theme	growth	630:635	arg1	cytokines					557:565	inflammatory cytokines	544:565	inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10)	544:664	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	1	theme	growth	630:635	arg1	TGF-β					647:651	TGF-β	647:651	TGF-β	647:651	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	1	theme	growth	630:635	arg1	factor-β					637:644	transforming growth factor-β	617:644	transforming growth factor-β (TGF-β)	617:652	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	6	2	theme	gut	1176:1178	arg1	microbiota					1180:1189	the gut microbiota	1172:1189	the gut microbiota	1172:1189	In addition, TWE and TES treatment caused significant alterations in the gut microbiota.
36453715	3	3	theme	activity	453:460	arg1	status					462:467	Physiological activity status	439:467	Physiological activity status	439:467	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	5	4	theme	cytokine	1087:1094	arg1	IL-10					1096:1100	the anti-inflammatory cytokine IL-10	1065:1100	the anti-inflammatory cytokine IL-10	1065:1100	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	1	5	theme	oolong	175:180	arg1	Tieguanyin					187:196	Tieguanyin	187:196	Tieguanyin	187:196	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	1	5	theme	oolong	175:180	arg1	tea					182:184	oolong tea	175:184	oolong tea	175:184	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	3	6	theme	dextran	747:753	arg1	DSS					771:773	DSS	771:773	DSS	771:773	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	6	theme	dextran	747:753	arg1	sodium					763:768	dextran sulfate sodium	747:768	dextran sulfate sodium (DSS)	747:774	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	7	theme	experimental	710:721	arg1	mice					731:734	experimental colitis mice	710:734	experimental colitis mice induced by dextran sulfate sodium (DSS)	710:774	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	7	8	theme	pernicious	1256:1265	arg1	bacteria					1267:1274	pernicious bacteria	1256:1274	pernicious bacteria	1256:1274	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	3	9	theme	disease	488:494	arg1	DAI					512:514	DAI	512:514	DAI	512:514	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	9	theme	disease	488:494	arg1	index					505:509	disease activity index	488:509	disease activity index (DAI)	488:515	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	10	theme	sulfate	755:761	arg1	DSS					771:773	DSS	771:773	DSS	771:773	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	10	theme	sulfate	755:761	arg1	sodium					763:768	dextran sulfate sodium	747:768	dextran sulfate sodium (DSS)	747:774	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	7	11	theme	2.2	1411:1413	arg1	%					1414:1414	%	1414:1414	%	1414:1414	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	4	12	theme	protective	808:817	arg1	effects					819:825	remarkable protective effects	797:825	remarkable protective effects	797:825	TWE and TES exerted remarkable protective effects against experimental colitis, showing decreased colitis severity and improved colon morphology.
36453715	5	13	theme	cytokines	1003:1011	arg1	downregulation					968:981	downregulation	968:981	downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β)	968:1043	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	13	theme	cytokines	1003:1011	arg1	upregulation					1049:1060	upregulation	1049:1060	upregulation of the anti-inflammatory cytokine IL-10	1049:1100	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	3	14	theme	activity	496:503	arg1	DAI					512:514	DAI	512:514	DAI	512:514	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	14	theme	activity	496:503	arg1	index					505:509	disease activity index	488:509	disease activity index (DAI)	488:515	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	7	15	theme	15.2	1419:1422	arg1	%					1414:1414	%	1414:1414	%	1414:1414	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	4	16	theme	experimental	835:846	arg1	colitis					848:854	experimental colitis	835:854	experimental colitis	835:854	TWE and TES exerted remarkable protective effects against experimental colitis, showing decreased colitis severity and improved colon morphology.
36453715	3	17	theme	transforming	617:628	arg1	cytokines					557:565	inflammatory cytokines	544:565	inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10)	544:664	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	17	theme	transforming	617:628	arg1	TGF-β					647:651	TGF-β	647:651	TGF-β	647:651	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	17	theme	transforming	617:628	arg1	factor-β					637:644	transforming growth factor-β	617:644	transforming growth factor-β (TGF-β)	617:652	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	6	18	theme	significant	1145:1155	arg1	alterations					1157:1167	significant alterations	1145:1167	significant alterations in the gut microbiota	1145:1189	In addition, TWE and TES treatment caused significant alterations in the gut microbiota.
36453715	2	19	theme	water	392:396	arg1	effects					370:376	the alleviation effects	354:376	the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD	354:436	In this study, we aimed to explore the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD.
36453715	8	20	dep	suggested	1526:1534	arg1	candidate					1563:1571	a candidate	1561:1571	a candidate for IBD treatment	1561:1589	Therefore, TWE and TES both remarkably ameliorated DSS-induced colitis, which suggested oolong extracts could be a candidate for IBD treatment.
36453715	1	21	theme	tea	182:184	arg1	kind					167:170	a typical kind	157:170	a typical kind of oolong tea, Tieguanyin,	157:197	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	7	22	theme	tea	1199:1201	arg1	treatment					1211:1219	Oolong tea extract treatment	1192:1219	Oolong tea extract treatment	1192:1219	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	4	23	theme	colon	905:909	arg1	morphology					911:920	improved colon morphology	896:920	improved colon morphology	896:920	TWE and TES exerted remarkable protective effects against experimental colitis, showing decreased colitis severity and improved colon morphology.
36453715	2	24	theme	alleviation	358:368	arg1	effects					370:376	the alleviation effects	354:376	the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD	354:436	In this study, we aimed to explore the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD.
36453715	2	25	theme	ethanol	408:414	arg1	effects					370:376	the alleviation effects	354:376	the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD	354:436	In this study, we aimed to explore the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD.
36453715	5	26	theme	anti-inflammatory	1069:1085	arg1	IL-10					1096:1100	the anti-inflammatory cytokine IL-10	1065:1100	the anti-inflammatory cytokine IL-10	1065:1100	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	1	27	from	effects	274:280	arg1	IBD					313:315	IBD	313:315	IBD	313:315	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	1	27	from	effects	274:280	arg1	disease					304:310	inflammatory bowel disease	285:310	inflammatory bowel disease (IBD)	285:316	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	7	28	theme	extract	1203:1209	arg1	treatment					1211:1219	Oolong tea extract treatment	1192:1219	Oolong tea extract treatment	1192:1219	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	0	29	theme	Tieguanyin	0:9	arg1	extracts					11:18	Tieguanyin extracts	0:18	Tieguanyin extracts	0:18	Tieguanyin extracts ameliorated DSS-induced mouse colitis by suppressing inflammation and regulating intestinal microbiota.
36453715	6	30	theme	TES	1124:1126	arg1	treatment					1128:1136	TWE and TES treatment	1116:1136	treatment	1128:1136	In addition, TWE and TES treatment caused significant alterations in the gut microbiota.
36453715	0	31	theme	DSS-induced	32:42	arg1	colitis					50:56	DSS-induced mouse colitis	32:56	DSS-induced mouse colitis	32:56	Tieguanyin extracts ameliorated DSS-induced mouse colitis by suppressing inflammation and regulating intestinal microbiota.
36453715	1	32	theme	Previous	124:131	arg1	studies					133:139	Previous studies	124:139	Previous studies	124:139	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	8	33	theme	oolong	1536:1541	arg1	extracts					1543:1550	oolong extracts	1536:1550	oolong extracts	1536:1550	Therefore, TWE and TES both remarkably ameliorated DSS-induced colitis, which suggested oolong extracts could be a candidate for IBD treatment.
36453715	7	34	theme	Oolong	1192:1197	arg1	treatment					1211:1219	Oolong tea extract treatment	1192:1219	Oolong tea extract treatment	1192:1219	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	1	35	theme	inflammatory	285:296	arg1	IBD					313:315	IBD	313:315	IBD	313:315	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	1	35	theme	inflammatory	285:296	arg1	disease					304:310	inflammatory bowel disease	285:310	inflammatory bowel disease (IBD)	285:316	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	8	36	theme	IBD	1577:1579	arg1	treatment					1581:1589	IBD treatment	1577:1589	IBD treatment	1577:1589	Therefore, TWE and TES both remarkably ameliorated DSS-induced colitis, which suggested oolong extracts could be a candidate for IBD treatment.
36453715	3	37	theme	interleukin	568:578	arg1	cytokines					557:565	inflammatory cytokines	544:565	inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10)	544:664	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	37	theme	interleukin	568:578	arg1	IL					581:582	interleukin (IL)-4	568:585	interleukin (IL)-4	568:585	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	1	38	theme	multiple	203:210	arg1	benefits					219:226	multiple health benefits	203:226	multiple health benefits	203:226	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	1	39	theme	bowel	298:302	arg1	IBD					313:315	IBD	313:315	IBD	313:315	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	1	39	theme	bowel	298:302	arg1	disease					304:310	inflammatory bowel disease	285:310	inflammatory bowel disease (IBD)	285:316	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	4	40	theme	remarkable	797:806	arg1	effects					819:825	remarkable protective effects	797:825	remarkable protective effects	797:825	TWE and TES exerted remarkable protective effects against experimental colitis, showing decreased colitis severity and improved colon morphology.
36453715	1	41	theme	health	212:217	arg1	benefits					219:226	multiple health benefits	203:226	multiple health benefits	203:226	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	2	42	theme	Tieguanyin	381:390	arg1	TWE					399:401	TWE	399:401	TWE	399:401	In this study, we aimed to explore the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD.
36453715	2	42	theme	Tieguanyin	381:390	arg1	water					392:396	Tieguanyin water	381:396	Tieguanyin water (TWE)	381:402	In this study, we aimed to explore the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD.
36453715	0	43	theme	mouse	44:48	arg1	colitis					50:56	DSS-induced mouse colitis	32:56	DSS-induced mouse colitis	32:56	Tieguanyin extracts ameliorated DSS-induced mouse colitis by suppressing inflammation and regulating intestinal microbiota.
36453715	4	44	theme	colitis	875:881	arg1	severity					883:890	decreased colitis severity	865:890	decreased colitis severity	865:890	TWE and TES exerted remarkable protective effects against experimental colitis, showing decreased colitis severity and improved colon morphology.
36453715	3	45	theme	spleen	528:533	arg1	weight					535:540	spleen weight	528:540	spleen weight	528:540	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	4	46	theme	improved	896:903	arg1	morphology					911:920	improved colon morphology	896:920	improved colon morphology	896:920	TWE and TES exerted remarkable protective effects against experimental colitis, showing decreased colitis severity and improved colon morphology.
36453715	7	47	theme	probiotics	1365:1374	arg1	Lachnospiraceae_NK4A136_group					1376:1404	probiotics Lachnospiraceae_NK4A136_group	1365:1404	probiotics Lachnospiraceae_NK4A136_group	1365:1404	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	5	48	theme	pro-inflammatory	986:1001	arg1	IL-4					1014:1017	IL-4	1014:1017	IL-4	1014:1017	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	48	theme	pro-inflammatory	986:1001	arg1	cytokines					1003:1011	pro-inflammatory cytokines	986:1011	pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β)	986:1043	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	48	theme	pro-inflammatory	986:1001	arg1	IL-17					1027:1031	IL-17	1027:1031	IL-17	1027:1031	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	48	theme	pro-inflammatory	986:1001	arg1	TGF-β					1038:1042	TGF-β	1038:1042	TGF-β	1038:1042	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	48	theme	pro-inflammatory	986:1001	arg1	IFN-γ					1020:1024	IFN-γ	1020:1024	IFN-γ	1020:1024	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	7	49	theme	bacteria	1267:1274	arg1	abundance					1243:1251	the community abundance	1229:1251	the community abundance of pernicious bacteria	1229:1274	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	3	50	dep	severity	478:485	arg1	DAI					512:514	DAI	512:514	DAI	512:514	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	50	dep	severity	478:485	arg1	index					505:509	disease activity index	488:509	disease activity index (DAI)	488:515	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	50	dep	severity	478:485	arg1	weight					535:540	spleen weight	528:540	spleen weight	528:540	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	50	dep	severity	478:485	arg1	colon					518:522	colon	518:522	colon	518:522	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	5	51	dep	cytokines	1003:1011	arg1	IL-4					1014:1017	IL-4	1014:1017	IL-4	1014:1017	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	51	dep	cytokines	1003:1011	arg1	cytokines					1003:1011	pro-inflammatory cytokines	986:1011	pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β)	986:1043	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	51	dep	cytokines	1003:1011	arg1	IL-17					1027:1031	IL-17	1027:1031	IL-17	1027:1031	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	51	dep	cytokines	1003:1011	arg1	TGF-β					1038:1042	TGF-β	1038:1042	TGF-β	1038:1042	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	51	dep	cytokines	1003:1011	arg1	IFN-γ					1020:1024	IFN-γ	1020:1024	IFN-γ	1020:1024	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	8	52	theme	DSS-induced	1499:1509	arg1	colitis					1511:1517	DSS-induced colitis	1499:1517	DSS-induced colitis	1499:1517	Therefore, TWE and TES both remarkably ameliorated DSS-induced colitis, which suggested oolong extracts could be a candidate for IBD treatment.
36453715	2	53	dep	water	392:396	arg1	extracts					422:429	extracts	422:429	extracts	422:429	In this study, we aimed to explore the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD.
36453715	2	54	from	effects	370:376	arg1	IBD					434:436	IBD	434:436	IBD	434:436	In this study, we aimed to explore the alleviation effects of Tieguanyin water (TWE) and ethanol (TES) extracts on IBD.
36453715	3	55	theme	Physiological	439:451	arg1	status					462:467	Physiological activity status	439:467	Physiological activity status	439:467	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	7	56	from	%	1306:1306	arg1	Escherichia-Shigella					1276:1295	Escherichia-Shigella	1276:1295	Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE)	1276:1341	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	7	57	dep	%	1414:1414	arg1	to					1416:1417	to	1416:1417	to	1416:1417	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	6	58	theme	TWE	1116:1118	arg1	treatment					1128:1136	TWE and TES treatment	1116:1136	treatment	1128:1136	In addition, TWE and TES treatment caused significant alterations in the gut microbiota.
36453715	3	59	dep	cytokines	557:565	arg1	IL-17					610:614	IL-17	610:614	IL-17	610:614	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	59	dep	cytokines	557:565	arg1	cytokines					557:565	inflammatory cytokines	544:565	inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10)	544:664	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	59	dep	cytokines	557:565	arg1	interferon-γ					588:599	interferon-γ (IFN-γ)	588:607	interferon-γ (IFN-γ)	588:607	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	59	dep	cytokines	557:565	arg1	IL-10					659:663	IL-10	659:663	IL-10	659:663	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	59	dep	cytokines	557:565	arg1	factor-β					637:644	transforming growth factor-β	617:644	transforming growth factor-β (TGF-β)	617:652	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	59	dep	cytokines	557:565	arg1	IL					581:582	interleukin (IL)-4	568:585	interleukin (IL)-4	568:585	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	59	dep	cytokines	557:565	arg1	TGF-β					647:651	TGF-β	647:651	TGF-β	647:651	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	60	theme	microbiota	670:679	arg1	composition					681:691	microbiota composition	670:691	microbiota composition	670:691	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	5	61	theme	colonic	943:949	arg1	inflammation					951:962	colonic inflammation	943:962	colonic inflammation	943:962	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	3	62	theme	inflammatory	544:555	arg1	IL-17					610:614	IL-17	610:614	IL-17	610:614	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	62	theme	inflammatory	544:555	arg1	cytokines					557:565	inflammatory cytokines	544:565	inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10)	544:664	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	62	theme	inflammatory	544:555	arg1	interferon-γ					588:599	interferon-γ (IFN-γ)	588:607	interferon-γ (IFN-γ)	588:607	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	62	theme	inflammatory	544:555	arg1	IL-10					659:663	IL-10	659:663	IL-10	659:663	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	62	theme	inflammatory	544:555	arg1	factor-β					637:644	transforming growth factor-β	617:644	transforming growth factor-β (TGF-β)	617:652	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	62	theme	inflammatory	544:555	arg1	IL					581:582	interleukin (IL)-4	568:585	interleukin (IL)-4	568:585	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	3	63	theme	colitis	723:729	arg1	mice					731:734	experimental colitis mice	710:734	experimental colitis mice induced by dextran sulfate sodium (DSS)	710:774	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	6	64	from	alterations	1157:1167	arg1	microbiota					1180:1189	the gut microbiota	1172:1189	the gut microbiota	1172:1189	In addition, TWE and TES treatment caused significant alterations in the gut microbiota.
36453715	1	65	contain	has	199:201	arg2	benefits					219:226	multiple health benefits	203:226	multiple health benefits	203:226	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	1	65	contain	has	199:201	arg1	kind					167:170	a typical kind	157:170	a typical kind of oolong tea, Tieguanyin,	157:197	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	7	66	theme	community	1233:1241	arg1	abundance					1243:1251	the community abundance	1229:1251	the community abundance of pernicious bacteria	1229:1274	Oolong tea extract treatment reduced the community abundance of pernicious bacteria Escherichia-Shigella from 21.6% (DSS) to 0.9% (TES) and 1.2% (TWE), and elevated that of probiotics Lachnospiraceae_NK4A136_group from 2.2% to 15.2% (TES) and 11.9% (TWE).
36453715	1	67	theme	typical	159:165	arg1	kind					167:170	a typical kind	157:170	a typical kind of oolong tea, Tieguanyin,	157:197	Previous studies have shown that a typical kind of oolong tea, Tieguanyin, has multiple health benefits, while there is no research investigating its effects on inflammatory bowel disease (IBD).
36453715	4	68	theme	decreased	865:873	arg1	severity					883:890	decreased colitis severity	865:890	decreased colitis severity	865:890	TWE and TES exerted remarkable protective effects against experimental colitis, showing decreased colitis severity and improved colon morphology.
36453715	3	69	theme	colitis	470:476	arg1	severity					478:485	colitis severity	470:485	colitis severity (disease activity index (DAI), colon and spleen weight)	470:541	Physiological activity status, colitis severity (disease activity index (DAI), colon and spleen weight), inflammatory cytokines (interleukin (IL)-4, interferon-γ (IFN-γ), IL-17, transforming growth factor-β (TGF-β), and IL-10) and microbiota composition were measured in experimental colitis mice induced by dextran sulfate sodium (DSS).
36453715	5	70	theme	IL-10	1096:1100	arg1	downregulation					968:981	downregulation	968:981	downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β)	968:1043	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
36453715	5	70	theme	IL-10	1096:1100	arg1	upregulation					1049:1060	upregulation	1049:1060	upregulation of the anti-inflammatory cytokine IL-10	1049:1100	TES also suppressed colonic inflammation via downregulation of pro-inflammatory cytokines (IL-4, IFN-γ, IL-17, and TGF-β) and upregulation of the anti-inflammatory cytokine IL-10.
35420503	16	0	theme	NSAIDs	2144:2149	arg1	Use					2137:2139	CONCLUSION Use	2126:2139	CONCLUSION Use of NSAIDs before hMSC injection	2126:2171	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	2	1	theme	recent	364:369	arg1	years					371:375	recent years	364:375	recent years without sufficient scientific evidence defining best-use practices	364:442	Clinical use of cell therapies for osteoarthritis has accelerated in recent years without sufficient scientific evidence defining best-use practices.
35420503	7	2	theme	meniscal	923:930	arg1	surgery					950:956	medial meniscal transection (MMT) surgery	916:956	medial meniscal transection (MMT) surgery	916:956	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	18	3	theme	CLINICAL	2435:2442	arg1	data					2458:2461	CLINICAL RELEVANCE Our data	2435:2461	CLINICAL RELEVANCE Our data	2435:2461	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	13	4	theme	other	1798:1802	arg1	differences					1816:1826	no other significant differences	1795:1826	no other significant differences	1795:1826	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	6	5	theme	STUDY	845:849	arg1	DESIGN					851:856	STUDY DESIGN	845:856	STUDY DESIGN	845:856	STUDY DESIGN Controlled laboratory study.
35420503	2	6	theme	Clinical	295:302	arg1	use					304:306	Clinical use	295:306	Clinical use of cell therapies for osteoarthritis	295:343	Clinical use of cell therapies for osteoarthritis has accelerated in recent years without sufficient scientific evidence defining best-use practices.
35420503	10	7	theme	von	1420:1422	arg1	filament					1429:1436	a von Frey filament	1418:1436	a von Frey filament	1418:1436	Pain (secondary allodynia) was measured using a von Frey filament.
35420503	7	8	theme	Lewis	895:899	arg1	rats					901:904	Lewis rats	895:904	Lewis rats	895:904	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	8	9	theme	experiment	1178:1187	arg1	course					1164:1169	the course	1160:1169	the course of the experiment (Full-NSAID group)	1160:1206	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	2	10	theme	therapies	316:324	arg1	use					304:306	Clinical use	295:306	Clinical use of cell therapies for osteoarthritis	295:343	Clinical use of cell therapies for osteoarthritis has accelerated in recent years without sufficient scientific evidence defining best-use practices.
35420503	8	11	theme	Full-NSAID	1190:1199	arg1	experiment					1178:1187	the experiment	1174:1187	the experiment (Full-NSAID group)	1174:1206	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	8	11	theme	Full-NSAID	1190:1199	arg1	group					1201:1205	Full-NSAID group	1190:1205	Full-NSAID group	1190:1205	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	8	12	theme	No	1243:1244	arg1	NSAIDs					1235:1240	NSAIDs	1235:1240	NSAIDs (No NSAID)	1235:1251	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	8	12	theme	No	1243:1244	arg1	NSAID					1246:1250	No NSAID	1243:1250	No NSAID	1243:1250	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	14	13	theme	hMSC	1911:1914	arg1	injections					1916:1925	hMSC injections	1911:1925	hMSC injections	1911:1925	Thus, pretreatment with NSAIDs had minimal effects on the therapeutic benefits of hMSC injections.
35420503	12	14	theme	osteophyte	1641:1650	arg1	formation					1652:1660	mineralized osteophyte formation	1629:1660	mineralized osteophyte formation	1629:1660	hMSCs prevented proteoglycan loss, maintained smooth cartilage surfaces, reduced cartilage lesions, reduced mineralized osteophyte formation, and reduced pain by week 7.
35420503	18	15	theme	therapeutic	2624:2634	arg1	efficacy					2636:2643	the therapeutic efficacy	2620:2643	the therapeutic efficacy of cell treatment	2620:2661	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	4	16	dep	Recommendations	632:646	arg1	restrict					651:658	restrict	651:658	to restrict NSAID use	648:668	Recommendations to restrict NSAID use are challenging for patients, and it is unclear if patients are compliant.
35420503	16	17	theme	therapeutic	2195:2205	arg1	benefits					2207:2214	the therapeutic benefits	2191:2214	the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes	2191:2321	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	14	18	contain	had	1860:1862	arg2	effects					1872:1878	minimal effects	1864:1878	minimal effects	1864:1878	Thus, pretreatment with NSAIDs had minimal effects on the therapeutic benefits of hMSC injections.
35420503	14	18	contain	had	1860:1862	arg1	pretreatment					1835:1846	pretreatment	1835:1846	pretreatment with NSAIDs	1835:1858	Thus, pretreatment with NSAIDs had minimal effects on the therapeutic benefits of hMSC injections.
35420503	3	19	dep	concerns	578:585	arg1	affect					603:608	affect	603:608	may affect therapeutic efficacy	599:629	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	16	20	from	preservation	2241:2252	arg1	osteophytes					2311:2321	osteophytes	2311:2321	osteophytes	2311:2321	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	12	21	theme	smooth	1567:1572	arg1	surfaces					1584:1591	smooth cartilage surfaces	1567:1591	smooth cartilage surfaces	1567:1591	hMSCs prevented proteoglycan loss, maintained smooth cartilage surfaces, reduced cartilage lesions, reduced mineralized osteophyte formation, and reduced pain by week 7.
35420503	13	22	contain	had	1711:1713	arg1	group					1705:1709	The Pre-NSAID group	1691:1709	The Pre-NSAID group	1691:1709	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	13	22	contain	had	1711:1713	arg2	levels					1738:1743	decreased proteoglycan levels	1715:1743	decreased proteoglycan levels	1715:1743	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	0	23	theme	Therapy	63:69	arg1	Efficacy					26:33	Therapeutic Efficacy	14:33	Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy	14:69	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	1	24	theme	human	163:167	arg1	hMSCs					196:200	hMSCs	196:200	hMSCs	196:200	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	1	24	theme	human	163:167	arg1	cells					189:193	human mesenchymal stromal cells	163:193	human mesenchymal stromal cells (hMSCs)	163:201	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	13	25	theme	decreased	1715:1723	arg1	levels					1738:1743	decreased proteoglycan levels	1715:1743	decreased proteoglycan levels	1715:1743	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	7	26	theme	group	989:993	arg1	surgery					996:1002	a sham (sham group) surgery	976:1002	a sham (sham group) surgery that did not progress to PTOA	976:1032	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	1	27	theme	stromal	181:187	arg1	hMSCs					196:200	hMSCs	196:200	hMSCs	196:200	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	1	27	theme	stromal	181:187	arg1	cells					189:193	human mesenchymal stromal cells	163:193	human mesenchymal stromal cells (hMSCs)	163:201	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	12	28	theme	proteoglycan	1537:1548	arg1	loss					1550:1553	proteoglycan loss	1537:1553	proteoglycan loss	1537:1553	hMSCs prevented proteoglycan loss, maintained smooth cartilage surfaces, reduced cartilage lesions, reduced mineralized osteophyte formation, and reduced pain by week 7.
35420503	8	29	theme	naproxen	1049:1056	arg1	solution					1058:1065	naproxen solution	1049:1065	naproxen solution	1049:1065	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	11	30	theme	RESULTS	1439:1445	arg1	Injection					1447:1455	RESULTS Injection	1439:1455	RESULTS Injection of hMSCs	1439:1464	RESULTS Injection of hMSCs attenuated cartilage degeneration associated with MMT.
35420503	2	31	theme	best-use	425:432	arg1	practices					434:442	best-use practices	425:442	best-use practices	425:442	Clinical use of cell therapies for osteoarthritis has accelerated in recent years without sufficient scientific evidence defining best-use practices.
35420503	5	32	theme	hMSC	791:794	arg1	therapy					796:802	hMSC therapy	791:802	hMSC therapy	791:802	HYPOTHESIS NSAIDs will reduce the efficacy of hMSC therapy in treating a preclinical model of PTOA.
35420503	0	33	theme	Mesenchymal	38:48	arg1	Therapy					63:69	Mesenchymal Stromal Cell Therapy	38:69	Mesenchymal Stromal Cell Therapy	38:69	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	16	34	theme	cartilage	2257:2265	arg1	integrity					2275:2283	cartilage surface integrity	2257:2283	cartilage surface integrity	2257:2283	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	15	35	theme	Full-NSAID	1947:1956	arg1	groups					1958:1963	The Post-NSAID and Full-NSAID groups	1928:1963	The Post-NSAID and Full-NSAID groups	1928:1963	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	9	36	theme	contrast-enhanced	1313:1329	arg1	tomography					1346:1355	contrast-enhanced micro-computed tomography	1313:1355	contrast-enhanced micro-computed tomography	1313:1355	Cartilage morphology and composition were quantified using contrast-enhanced micro-computed tomography and histology.
35420503	15	37	theme	surface	2078:2084	arg1	roughness					2086:2094	surface roughness	2078:2094	surface roughness	2078:2094	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	17	38	theme	treatment	2424:2432	arg1	efficacy					2403:2410	the therapeutic efficacy	2387:2410	the therapeutic efficacy of cellular treatment	2387:2432	Use of NSAIDs after injections, however, substantially reduced the therapeutic efficacy of cellular treatment.
35420503	3	39	theme	drug	524:527	arg1	use					537:539	nonsteroidal anti-inflammatory drug (NSAID) use	493:539	nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy	493:629	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	0	40	theme	Therapeutic	14:24	arg1	Efficacy					26:33	Therapeutic Efficacy	14:33	Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy	14:69	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	8	41	theme	Pre-NSAID	1088:1096	arg1	group					1098:1102	Pre-NSAID group	1088:1102	Pre-NSAID group	1088:1102	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	3	42	theme	Common	445:450	arg1	recommendations					452:466	Common recommendations	445:466	Common recommendations	445:466	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	18	43	theme	clinical	2475:2482	arg1	recommendation					2484:2497	the clinical recommendation	2471:2497	the clinical recommendation of avoiding NSAID use after hMSC injection	2471:2540	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	3	44	theme	nonsteroidal	493:504	arg1	use					537:539	nonsteroidal anti-inflammatory drug (NSAID) use	493:539	nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy	493:629	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	5	45	theme	PTOA	839:842	arg1	model					830:834	a preclinical model	816:834	a preclinical model of PTOA	816:842	HYPOTHESIS NSAIDs will reduce the efficacy of hMSC therapy in treating a preclinical model of PTOA.
35420503	18	46	theme	NSAID	2511:2515	arg1	use					2517:2519	avoiding NSAID use	2502:2519	avoiding NSAID use	2502:2519	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	3	47	theme	NSAID	530:534	arg1	use					537:539	nonsteroidal anti-inflammatory drug (NSAID) use	493:539	nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy	493:629	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	8	48	theme	Post-NSAID	1115:1124	arg1	treatment					1138:1146	(Post-NSAID group) hMSC treatment	1114:1146	(Post-NSAID group) hMSC treatment	1114:1146	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	8	48	theme	Post-NSAID	1115:1124	arg1	group					1126:1130	Post-NSAID group	1115:1130	Post-NSAID group	1115:1130	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	16	49	from	reduction	2298:2306	arg1	osteophytes					2311:2321	osteophytes	2311:2321	osteophytes	2311:2321	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	4	50	theme	NSAID	660:664	arg1	use					666:668	NSAID use	660:668	NSAID use	660:668	Recommendations to restrict NSAID use are challenging for patients, and it is unclear if patients are compliant.
35420503	3	51	theme	anti-inflammatory	506:522	arg1	use					537:539	nonsteroidal anti-inflammatory drug (NSAID) use	493:539	nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy	493:629	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	3	52	theme	cell	558:561	arg1	injection					563:571	cell injection	558:571	cell injection	558:571	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	16	53	theme	hMSC	2158:2161	arg1	injection					2163:2171	hMSC injection	2158:2171	hMSC injection	2158:2171	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	18	54	theme	cell	2648:2651	arg1	treatment					2653:2661	cell treatment	2648:2661	cell treatment	2648:2661	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	7	55	dep	METHODS	887:893	arg1	underwent					906:914	underwent	906:914	underwent medial meniscal transection (MMT) surgery	906:956	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	7	56	theme	MMT	945:947	arg1	surgery					950:956	medial meniscal transection (MMT) surgery	916:956	medial meniscal transection (MMT) surgery	916:956	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	1	57	theme	posttraumatic	258:270	arg1	osteoarthritis					272:285	posttraumatic osteoarthritis	258:285	posttraumatic osteoarthritis (PTOA)	258:292	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	1	57	theme	posttraumatic	258:270	arg1	PTOA					288:291	PTOA	288:291	PTOA	288:291	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	17	58	theme	therapeutic	2391:2401	arg1	efficacy					2403:2410	the therapeutic efficacy	2387:2410	the therapeutic efficacy of cellular treatment	2387:2432	Use of NSAIDs after injections, however, substantially reduced the therapeutic efficacy of cellular treatment.
35420503	7	59	theme	transection	932:942	arg1	surgery					950:956	medial meniscal transection (MMT) surgery	916:956	medial meniscal transection (MMT) surgery	916:956	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	13	60	theme	significant	1804:1814	arg1	differences					1816:1826	no other significant differences	1795:1826	no other significant differences	1795:1826	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	10	61	theme	secondary	1378:1386	arg1	Pain					1372:1375	Pain	1372:1375	Pain (secondary allodynia)	1372:1397	Pain (secondary allodynia) was measured using a von Frey filament.
35420503	10	61	theme	secondary	1378:1386	arg1	allodynia					1388:1396	secondary allodynia	1378:1396	secondary allodynia	1378:1396	Pain (secondary allodynia) was measured using a von Frey filament.
35420503	7	62	theme	medial	916:921	arg1	surgery					950:956	medial meniscal transection (MMT) surgery	916:956	medial meniscal transection (MMT) surgery	916:956	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	1	63	theme	cells	189:193	arg1	injections					149:158	BACKGROUND Intra-articular injections	122:158	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs)	122:201	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	0	64	theme	Stromal	50:56	arg1	Therapy					63:69	Mesenchymal Stromal Cell Therapy	38:69	Mesenchymal Stromal Cell Therapy	38:69	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	15	65	theme	Post-NSAID	1932:1941	arg1	groups					1958:1963	The Post-NSAID and Full-NSAID groups	1928:1963	The Post-NSAID and Full-NSAID groups	1928:1963	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	3	66	theme	therapeutic	610:620	arg1	efficacy					622:629	therapeutic efficacy	610:629	therapeutic efficacy	610:629	Common recommendations advise patients to avoid nonsteroidal anti-inflammatory drug (NSAID) use before and after cell injection over concerns that NSAIDs may affect therapeutic efficacy.
35420503	18	67	theme	use	2517:2519	arg1	recommendation					2484:2497	the clinical recommendation	2471:2497	the clinical recommendation of avoiding NSAID use after hMSC injection	2471:2540	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	14	68	with	pretreatment	1835:1846	arg1	NSAIDs					1853:1858	NSAIDs	1853:1858	NSAIDs	1853:1858	Thus, pretreatment with NSAIDs had minimal effects on the therapeutic benefits of hMSC injections.
35420503	12	69	theme	mineralized	1629:1639	arg1	formation					1652:1660	mineralized osteophyte formation	1629:1660	mineralized osteophyte formation	1629:1660	hMSCs prevented proteoglycan loss, maintained smooth cartilage surfaces, reduced cartilage lesions, reduced mineralized osteophyte formation, and reduced pain by week 7.
35420503	15	70	theme	worse	1999:2003	arg1	osteoarthritis					2005:2018	significantly worse osteoarthritis	1985:2018	significantly worse osteoarthritis	1985:2018	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	5	71	theme	HYPOTHESIS	745:754	arg1	NSAIDs					756:761	HYPOTHESIS NSAIDs	745:761	HYPOTHESIS NSAIDs	745:761	HYPOTHESIS NSAIDs will reduce the efficacy of hMSC therapy in treating a preclinical model of PTOA.
35420503	2	72	theme	cell	311:314	arg1	therapies					316:324	cell therapies	311:324	cell therapies	311:324	Clinical use of cell therapies for osteoarthritis has accelerated in recent years without sufficient scientific evidence defining best-use practices.
35420503	14	73	theme	injections	1916:1925	arg1	benefits					1899:1906	the therapeutic benefits	1883:1906	the therapeutic benefits of hMSC injections	1883:1925	Thus, pretreatment with NSAIDs had minimal effects on the therapeutic benefits of hMSC injections.
35420503	18	74	theme	RELEVANCE	2444:2452	arg1	data					2458:2461	CLINICAL RELEVANCE Our data	2435:2461	CLINICAL RELEVANCE Our data	2435:2461	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	12	75	theme	cartilage	1602:1610	arg1	lesions					1612:1618	cartilage lesions	1602:1618	cartilage lesions	1602:1618	hMSCs prevented proteoglycan loss, maintained smooth cartilage surfaces, reduced cartilage lesions, reduced mineralized osteophyte formation, and reduced pain by week 7.
35420503	13	76	theme	hMSC	1763:1766	arg1	group					1768:1772	the hMSC group	1759:1772	the hMSC group	1759:1772	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	1	77	from	degradation	243:253	arg1	osteoarthritis					272:285	posttraumatic osteoarthritis	258:285	posttraumatic osteoarthritis (PTOA)	258:292	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	1	77	from	degradation	243:253	arg1	PTOA					288:291	PTOA	288:291	PTOA	288:291	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	14	78	theme	therapeutic	1887:1897	arg1	benefits					1899:1906	the therapeutic benefits	1883:1906	the therapeutic benefits of hMSC injections	1883:1925	Thus, pretreatment with NSAIDs had minimal effects on the therapeutic benefits of hMSC injections.
35420503	17	79	theme	NSAIDs	2331:2336	arg1	Use					2324:2326	Use	2324:2326	Use of NSAIDs after injections	2324:2353	Use of NSAIDs after injections, however, substantially reduced the therapeutic efficacy of cellular treatment.
35420503	1	80	theme	Intra-articular	133:147	arg1	injections					149:158	BACKGROUND Intra-articular injections	122:158	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs)	122:201	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	12	81	theme	cartilage	1574:1582	arg1	surfaces					1584:1591	smooth cartilage surfaces	1567:1591	smooth cartilage surfaces	1567:1591	hMSCs prevented proteoglycan loss, maintained smooth cartilage surfaces, reduced cartilage lesions, reduced mineralized osteophyte formation, and reduced pain by week 7.
35420503	11	82	theme	cartilage	1477:1485	arg1	degeneration					1487:1498	cartilage degeneration	1477:1498	cartilage degeneration associated with MMT	1477:1518	RESULTS Injection of hMSCs attenuated cartilage degeneration associated with MMT.
35420503	5	83	theme	therapy	796:802	arg1	efficacy					779:786	the efficacy	775:786	the efficacy of hMSC therapy in treating a preclinical model of PTOA	775:842	HYPOTHESIS NSAIDs will reduce the efficacy of hMSC therapy in treating a preclinical model of PTOA.
35420503	14	84	theme	minimal	1864:1870	arg1	effects					1872:1878	minimal effects	1864:1878	minimal effects	1864:1878	Thus, pretreatment with NSAIDs had minimal effects on the therapeutic benefits of hMSC injections.
35420503	1	85	theme	BACKGROUND	122:131	arg1	injections					149:158	BACKGROUND Intra-articular injections	122:158	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs)	122:201	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	0	86	theme	Rodent	76:81	arg1	Model					83:87	a Rodent Model	74:87	a Rodent Model of Posttraumatic Osteoarthritis	74:119	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	9	87	theme	Cartilage	1254:1262	arg1	morphology					1264:1273	Cartilage morphology	1254:1273	Cartilage morphology	1254:1273	Cartilage morphology and composition were quantified using contrast-enhanced micro-computed tomography and histology.
35420503	1	88	theme	mesenchymal	169:179	arg1	hMSCs					196:200	hMSCs	196:200	hMSCs	196:200	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	1	88	theme	mesenchymal	169:179	arg1	cells					189:193	human mesenchymal stromal cells	163:193	human mesenchymal stromal cells (hMSCs)	163:201	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	13	89	theme	proteoglycan	1725:1736	arg1	levels					1738:1743	decreased proteoglycan levels	1715:1743	decreased proteoglycan levels	1715:1743	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	18	90	theme	hMSC	2527:2530	arg1	injection					2532:2540	hMSC injection	2527:2540	hMSC injection	2527:2540	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	15	91	theme	osteophyte	2097:2106	arg1	volume					2108:2113	osteophyte volume	2097:2113	osteophyte volume	2097:2113	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	10	92	theme	Frey	1424:1427	arg1	filament					1429:1436	a von Frey filament	1418:1436	a von Frey filament	1418:1436	Pain (secondary allodynia) was measured using a von Frey filament.
35420503	16	93	theme	integrity	2275:2283	arg1	preservation					2241:2252	preservation	2241:2252	preservation of cartilage surface integrity as well as a reduction in osteophytes	2241:2321	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	16	93	theme	integrity	2275:2283	arg1	reduction					2298:2306	a reduction	2296:2306	preservation of cartilage surface integrity as well as a reduction in osteophytes	2241:2321	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	0	94	theme	Osteoarthritis	106:119	arg1	Model					83:87	a Rodent Model	74:87	a Rodent Model of Posttraumatic Osteoarthritis	74:119	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	7	95	theme	sham	984:987	arg1	surgery					996:1002	a sham (sham group) surgery	976:1002	a sham (sham group) surgery that did not progress to PTOA	976:1032	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	13	96	theme	Pre-NSAID	1695:1703	arg1	group					1705:1709	The Pre-NSAID group	1691:1709	The Pre-NSAID group	1691:1709	The Pre-NSAID group had decreased proteoglycan levels compared with the hMSC group, although there were no other significant differences.
35420503	7	97	theme	sham	978:981	arg1	surgery					996:1002	a sham (sham group) surgery	976:1002	a sham (sham group) surgery that did not progress to PTOA	976:1032	METHODS Lewis rats underwent medial meniscal transection (MMT) surgery to induce PTOA or a sham (sham group) surgery that did not progress to PTOA.
35420503	17	98	theme	cellular	2415:2422	arg1	treatment					2424:2432	cellular treatment	2415:2432	cellular treatment	2415:2432	Use of NSAIDs after injections, however, substantially reduced the therapeutic efficacy of cellular treatment.
35420503	11	99	theme	hMSCs	1460:1464	arg1	Injection					1447:1455	RESULTS Injection	1439:1455	RESULTS Injection of hMSCs	1439:1464	RESULTS Injection of hMSCs attenuated cartilage degeneration associated with MMT.
35420503	2	100	theme	scientific	396:405	arg1	evidence					407:414	sufficient scientific evidence	385:414	sufficient scientific evidence defining best-use practices	385:442	Clinical use of cell therapies for osteoarthritis has accelerated in recent years without sufficient scientific evidence defining best-use practices.
35420503	9	101	theme	micro-computed	1331:1344	arg1	tomography					1346:1355	contrast-enhanced micro-computed tomography	1313:1355	contrast-enhanced micro-computed tomography	1313:1355	Cartilage morphology and composition were quantified using contrast-enhanced micro-computed tomography and histology.
35420503	15	102	theme	proteoglycan	2059:2070	arg1	loss					2072:2075	greater proteoglycan loss	2051:2075	greater proteoglycan loss	2051:2075	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	15	103	theme	greater	2051:2057	arg1	loss					2072:2075	greater proteoglycan loss	2051:2075	greater proteoglycan loss	2051:2075	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	2	104	theme	sufficient	385:394	arg1	evidence					407:414	sufficient scientific evidence	385:414	sufficient scientific evidence defining best-use practices	385:442	Clinical use of cell therapies for osteoarthritis has accelerated in recent years without sufficient scientific evidence defining best-use practices.
35420503	5	105	theme	preclinical	818:828	arg1	model					830:834	a preclinical model	816:834	a preclinical model of PTOA	816:842	HYPOTHESIS NSAIDs will reduce the efficacy of hMSC therapy in treating a preclinical model of PTOA.
35420503	16	106	theme	surface	2267:2273	arg1	integrity					2275:2283	cartilage surface integrity	2257:2283	cartilage surface integrity	2257:2283	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	0	107	theme	Posttraumatic	92:104	arg1	Osteoarthritis					106:119	Posttraumatic Osteoarthritis	92:119	Posttraumatic Osteoarthritis	92:119	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	0	108	theme	Cell	58:61	arg1	Therapy					63:69	Mesenchymal Stromal Cell Therapy	38:69	Mesenchymal Stromal Cell Therapy	38:69	NSAIDs Reduce Therapeutic Efficacy of Mesenchymal Stromal Cell Therapy in a Rodent Model of Posttraumatic Osteoarthritis.
35420503	15	109	theme	hMSC-only	2029:2037	arg1	group					2039:2043	the hMSC-only group	2025:2043	the hMSC-only group	2025:2043	The Post-NSAID and Full-NSAID groups, however, exhibited significantly worse osteoarthritis than the hMSC-only group, with greater proteoglycan loss, surface roughness, osteophyte volume, and pain.
35420503	16	110	theme	CONCLUSION	2126:2135	arg1	Use					2137:2139	CONCLUSION Use	2126:2139	CONCLUSION Use of NSAIDs before hMSC injection	2126:2171	CONCLUSION Use of NSAIDs before hMSC injection minimally reduced the therapeutic benefits for PTOA, which included preservation of cartilage surface integrity as well as a reduction in osteophytes.
35420503	18	111	theme	avoiding	2502:2509	arg1	use					2517:2519	avoiding NSAID use	2502:2519	avoiding NSAID use	2502:2519	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35420503	1	112	theme	cartilage	233:241	arg1	degradation					243:253	cartilage degradation	233:253	cartilage degradation in posttraumatic osteoarthritis (PTOA)	233:292	BACKGROUND Intra-articular injections of human mesenchymal stromal cells (hMSCs) have shown promise in slowing cartilage degradation in posttraumatic osteoarthritis (PTOA).
35420503	8	113	theme	hMSC	1133:1136	arg1	treatment					1138:1146	(Post-NSAID group) hMSC treatment	1114:1146	(Post-NSAID group) hMSC treatment	1114:1146	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	8	113	theme	hMSC	1133:1136	arg1	group					1126:1130	Post-NSAID group	1115:1130	Post-NSAID group	1115:1130	Rats received naproxen solution orally daily before (Pre-NSAID group) or after (Post-NSAID group) hMSC treatment, throughout the course of the experiment (Full-NSAID group), or received hMSCs without NSAIDs (No NSAID).
35420503	6	114	theme	laboratory	869:878	arg1	study					880:884	laboratory study	869:884	laboratory study	869:884	STUDY DESIGN Controlled laboratory study.
35420503	18	115	theme	treatment	2653:2661	arg1	efficacy					2636:2643	the therapeutic efficacy	2620:2643	the therapeutic efficacy of cell treatment	2620:2661	CLINICAL RELEVANCE Our data support the clinical recommendation of avoiding NSAID use after hMSC injection but suggest that using NSAIDs before treatment may not substantially diminish the therapeutic efficacy of cell treatment.
35963104	4	0	theme	Ad	786:787	arg1	group					797:801	Ad Libitum group	786:801	Ad Libitum group	786:801	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	9	1	theme	protein	1612:1618	arg1	content					1620:1626	milk protein content	1607:1626	milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk)	1607:1663	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	4	2	theme	access	921:926	arg1	time					928:931	access time	921:931	access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR)	921:1036	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	8	3	theme	Milk	1417:1420	arg1	yield					1422:1426	Milk yield	1417:1426	Milk yield	1417:1426	Milk yield was not significantly different among the three groups.
35963104	9	4	dep	fat	1562:1564	arg1	32.9					1575:1578	32.9	1575:1578	32.9	1575:1578	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	4	dep	fat	1562:1564	arg1	35.9					1567:1570	35.9	1567:1570	35.9	1567:1570	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	4	dep	fat	1562:1564	arg1	milk					1597:1600	milk	1597:1600	milk	1597:1600	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	3	5	theme	Holstein	522:529	arg1	cows					531:534	Holstein cows	522:534	Holstein cows	522:534	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	1	6	theme	stock	237:241	arg1	shortages					243:251	forage stock shortages	230:251	forage stock shortages	230:251	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	10	7	theme	non-esterified	1790:1803	arg1	concentration					1816:1828	a lower blood non-esterified fatty acid concentration	1776:1828	a lower blood non-esterified fatty acid concentration than the AL group	1776:1846	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	10	8	theme	lower	1778:1782	arg1	concentration					1816:1828	a lower blood non-esterified fatty acid concentration	1776:1828	a lower blood non-esterified fatty acid concentration than the AL group	1776:1846	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	10	9	theme	QTR	1673:1675	arg1	cows					1677:1680	QTR cows	1673:1680	QTR cows	1673:1680	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	4	10	dep	10.5 kg/day	883:893	arg1	iii					878:880	iii	878:880	iii	878:880	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	11	from	2 h	975:977	arg1	QTR					1033:1035	Quantity-and-Time-restricted group; QTR	997:1035	Quantity-and-Time-restricted group; QTR	997:1035	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	11	from	2 h	975:977	arg1	afternoon					986:994	the afternoon	982:994	the afternoon (Quantity-and-Time-restricted group; QTR)	982:1036	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	5	12	dep	status	1107:1112	arg1	metabolites					1151:1161	blood metabolites	1145:1161	blood metabolites	1145:1161	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	5	12	dep	status	1107:1112	arg1	weight					1115:1120	weight	1115:1120	weight	1115:1120	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	5	12	dep	status	1107:1112	arg1	score					1138:1142	body condition score	1123:1142	body condition score	1123:1142	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	4	13	from	morning	963:969	arg1	QTR					1033:1035	Quantity-and-Time-restricted group; QTR	997:1035	Quantity-and-Time-restricted group; QTR	997:1035	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	13	from	morning	963:969	arg1	afternoon					986:994	the afternoon	982:994	the afternoon (Quantity-and-Time-restricted group; QTR)	982:1036	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	10	14	theme	acid	1811:1814	arg1	concentration					1816:1828	a lower blood non-esterified fatty acid concentration	1776:1828	a lower blood non-esterified fatty acid concentration than the AL group	1776:1846	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	10	15	theme	body	1698:1701	arg1	fat					1703:1705	their body fat	1692:1705	their body fat	1692:1705	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	0	16	theme	dairy	118:122	arg1	performance					124:134	dairy performance	118:134	dairy performance	118:134	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	11	17	from	cows	1951:1954	arg1	different					1933:1941	different	1933:1941	different	1933:1941	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	14	18	theme	certain	2298:2304	arg1	hazards					2315:2321	certain climatic hazards	2298:2321	certain climatic hazards	2298:2321	This practice can be of interest in grassland systems to overcome certain climatic hazards without having to resort to purchases or to increase the farm's forage autonomy.
35963104	12	19	theme	protein	2004:2010	arg1	content					2012:2018	protein content	2004:2018	protein content	2004:2018	Access-time restriction did not impact fat and protein content but led to decreased casein, lactose contents and casein-to-whey protein ratio.
35963104	11	20	theme	AL	1948:1949	arg1	cows					1951:1954	AL cows	1948:1954	AL cows	1948:1954	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	7	21	theme	feed	1385:1388	arg1	efficiency					1390:1399	feed efficiency	1385:1399	feed efficiency	1385:1399	Organic matter digestibility was lower in the AL group than in the QR and QTR groups whereas feed efficiency did not differ.
35963104	10	22	theme	AL	1839:1840	arg1	group					1842:1846	the AL group	1835:1846	the AL group	1835:1846	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	5	23	theme	body	1123:1126	arg1	weight					1115:1120	weight	1115:1120	weight	1115:1120	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	5	23	theme	body	1123:1126	arg1	score					1138:1142	body condition score	1123:1142	body condition score	1123:1142	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	0	24	theme	cows	145:148	arg1	performance					124:134	dairy performance	118:134	dairy performance	118:134	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	0	24	theme	cows	145:148	arg1	behaviour					66:74	feeding behaviour	58:74	feeding behaviour	58:74	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	0	24	theme	cows	145:148	arg1	efficiency					82:91	feed efficiency	77:91	feed efficiency	77:91	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	0	24	theme	cows	145:148	arg1	status					106:111	nutritional status	94:111	nutritional status	94:111	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	14	25	theme	grassland	2268:2276	arg1	systems					2278:2284	grassland systems	2268:2284	grassland systems	2268:2284	This practice can be of interest in grassland systems to overcome certain climatic hazards without having to resort to purchases or to increase the farm's forage autonomy.
35963104	0	26	theme	feeding	58:64	arg1	behaviour					66:74	feeding behaviour	58:74	feeding behaviour	58:74	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	1	27	theme	biggest	295:301	arg1	cost					313:316	the biggest operating cost	291:316	the biggest operating cost	291:316	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	1	27	theme	biggest	295:301	arg1	feed					283:286	feed	283:286	feed	283:286	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	3	28	theme	cows	584:587	arg1	groups					569:574	three groups	563:574	three groups of nine cows balanced by breed, parity, days in milk, and milk yield	563:643	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	3	28	theme	cows	584:587	arg1	cows					584:587	nine cows	579:587	nine cows balanced by breed, parity, days in milk, and milk yield	579:643	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	2	29	theme	animal	443:448	arg1	status					462:467	animal nutritional status	443:467	animal nutritional status	443:467	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	4	30	theme	group	1026:1030	arg1	QTR					1033:1035	Quantity-and-Time-restricted group; QTR	997:1035	Quantity-and-Time-restricted group; QTR	997:1035	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	30	theme	group	1026:1030	arg1	afternoon					986:994	the afternoon	982:994	the afternoon (Quantity-and-Time-restricted group; QTR)	982:1036	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	14	31	dep	farm	2380:2383	arg1	forage					2387:2392	forage	2387:2392	forage autonomy	2387:2401	This practice can be of interest in grassland systems to overcome certain climatic hazards without having to resort to purchases or to increase the farm's forage autonomy.
35963104	10	32	theme	body	1735:1738	arg1	score					1750:1754	a lower final body condition score	1721:1754	a lower final body condition score	1721:1754	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	4	33	theme	hay	838:840	arg1	hay					781:783	first-cut hay	771:783	first-cut hay (Ad Libitum group; AL)	771:806	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	33	theme	hay	838:840	arg1	10.5 kg/day					813:823	ii) 10.5 kg/day	809:823	ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR)	809:872	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	33	theme	hay	838:840	arg1	10.5 kg/day					883:893	iii) 10.5 kg/day	878:893	iii) 10.5 kg/day of first-cut hay	878:910	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	0	34	from	Effects	0:6	arg1	performance					124:134	dairy performance	118:134	dairy performance	118:134	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	0	34	from	Effects	0:6	arg1	behaviour					66:74	feeding behaviour	58:74	feeding behaviour	58:74	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	0	34	from	Effects	0:6	arg1	efficiency					82:91	feed efficiency	77:91	feed efficiency	77:91	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	0	34	from	Effects	0:6	arg1	status					106:111	nutritional status	94:111	nutritional status	94:111	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	4	35	theme	hay	702:704	arg1	DM/day					681:686	3.9 kg DM/day	674:686	3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access	674:766	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	10	36	theme	lower	1723:1727	arg1	score					1750:1754	a lower final body condition score	1721:1754	a lower final body condition score	1721:1754	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	4	37	dep	10.5 kg/day	813:823	arg1	ii					809:810	ii	809:810	ii	809:810	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	2	38	theme	indoor	476:481	arg1	feeding					483:489	indoor feeding	476:489	indoor feeding	476:489	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	4	39	theme	4.5 kg/day	710:719	arg1	DM/day					681:686	3.9 kg DM/day	674:686	3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access	674:766	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	5	40	theme	cow	1168:1170	arg1	activities					1172:1181	cow activities	1168:1181	cow activities	1168:1181	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	0	41	theme	nutritional	94:104	arg1	status					106:111	nutritional status	94:111	nutritional status	94:111	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	0	42	theme	access-time	31:41	arg1	restriction					43:53	access-time restriction	31:53	access-time restriction	31:53	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	9	43	dep	content	1620:1626	arg1	27.7					1637:1640	27.7	1637:1640	27.7	1637:1640	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	43	dep	content	1620:1626	arg1	29.5					1629:1632	29.5	1629:1632	29.5	1629:1632	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	43	dep	content	1620:1626	arg1	milk					1659:1662	milk	1659:1662	milk	1659:1662	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	2	44	theme	access	406:411	arg1	time					413:416	access time	406:416	access time	406:416	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	4	45	theme	3.9 kg	674:679	arg1	DM/day					681:686	3.9 kg DM/day	674:686	3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access	674:766	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	46	theme	hay	908:910	arg1	hay					781:783	first-cut hay	771:783	first-cut hay (Ad Libitum group; AL)	771:806	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	46	theme	hay	908:910	arg1	10.5 kg/day					813:823	ii) 10.5 kg/day	809:823	ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR)	809:872	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	46	theme	hay	908:910	arg1	10.5 kg/day					883:893	iii) 10.5 kg/day	878:893	iii) 10.5 kg/day of first-cut hay	878:910	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	5	47	theme	blood	1145:1149	arg1	metabolites					1151:1161	blood metabolites	1145:1161	blood metabolites	1145:1161	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	5	47	theme	blood	1145:1149	arg1	weight					1115:1120	weight	1115:1120	weight	1115:1120	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	12	48	theme	decreased	2031:2039	arg1	casein					2041:2046	decreased casein	2031:2046	decreased casein	2031:2046	Access-time restriction did not impact fat and protein content but led to decreased casein, lactose contents and casein-to-whey protein ratio.
35963104	6	49	theme	QR	1244:1245	arg1	group					1247:1251	the QR group	1240:1251	the QR group	1240:1251	The AL group ingested 10 % more feed than the QR group and 16 % more feed than the QTR group.
35963104	11	50	theme	greater	1865:1871	arg1	mobilisation					1882:1893	greater body fat mobilisation	1865:1893	greater body fat mobilisation	1865:1893	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	11	51	theme	body	1873:1876	arg1	fat					1878:1880	body fat	1873:1880	greater body fat mobilisation	1865:1893	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	4	52	theme	group	863:867	arg1	QR					870:871	Quantity-restricted group; QR	843:871	Quantity-restricted group; QR	843:871	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	52	theme	group	863:867	arg1	hay					838:840	first-cut hay	828:840	first-cut hay (Quantity-restricted group; QR)	828:872	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	53	theme	libitum	753:759	arg1	hay					702:704	second-cut hay	691:704	second-cut hay	691:704	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	53	theme	libitum	753:759	arg1	4.5 kg/day					710:719	4.5 kg/day	710:719	4.5 kg/day	710:719	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	0	54	theme	restriction	43:53	arg1	Effects					0:6	Effects	0:6	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows	0:148	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	12	55	theme	protein	2085:2091	arg1	ratio					2093:2097	casein-to-whey protein ratio	2070:2097	casein-to-whey protein ratio	2070:2097	Access-time restriction did not impact fat and protein content but led to decreased casein, lactose contents and casein-to-whey protein ratio.
35963104	4	56	theme	concentrate	724:734	arg1	hay					702:704	second-cut hay	691:704	second-cut hay	691:704	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	56	theme	concentrate	724:734	arg1	4.5 kg/day					710:719	4.5 kg/day	710:719	4.5 kg/day	710:719	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	5	57	theme	coagulation	1067:1077	arg1	properties					1079:1088	coagulation properties	1067:1088	coagulation properties	1067:1088	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	7	58	theme	Organic	1292:1298	arg1	digestibility					1307:1319	Organic matter digestibility	1292:1319	Organic matter digestibility	1292:1319	Organic matter digestibility was lower in the AL group than in the QR and QTR groups whereas feed efficiency did not differ.
35963104	2	59	from	effects	366:372	arg1	status					462:467	animal nutritional status	443:467	animal nutritional status	443:467	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	2	59	from	effects	366:372	arg1	performance					427:437	dairy performance	421:437	dairy performance	421:437	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	3	60	from	days	616:619	arg1	milk					624:627	milk	624:627	milk	624:627	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	1	61	theme	livestock	208:216	arg1	systems					218:224	dairy livestock systems	202:224	dairy livestock systems	202:224	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	9	62	theme	milk	1607:1610	arg1	content					1620:1626	milk protein content	1607:1626	milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk)	1607:1663	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	12	63	theme	lactose	2049:2055	arg1	contents					2057:2064	lactose contents	2049:2064	lactose contents	2049:2064	Access-time restriction did not impact fat and protein content but led to decreased casein, lactose contents and casein-to-whey protein ratio.
35963104	11	64	theme	fat	1878:1880	arg1	mobilisation					1882:1893	greater body fat mobilisation	1865:1893	greater body fat mobilisation	1865:1893	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	13	65	theme	prices	2215:2220	arg1	increase					2222:2229	prices increase	2215:2229	prices increase	2215:2229	The forage savings achieved through this feed management practice could prove economically substantial when forage prices increase.
35963104	6	66	theme	feed	1267:1270	arg1	%					1260:1260	16 %	1257:1260	16 % more feed	1257:1270	The AL group ingested 10 % more feed than the QR group and 16 % more feed than the QTR group.
35963104	3	67	from	breed	601:605	arg1	milk					624:627	milk	624:627	milk	624:627	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	3	68	theme	milk	634:637	arg1	yield					639:643	milk yield	634:643	milk yield	634:643	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	4	69	theme	Libitum	789:795	arg1	group					797:801	Ad Libitum group	786:801	Ad Libitum group	786:801	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	10	70	theme	fatty	1805:1809	arg1	concentration					1816:1828	a lower blood non-esterified fatty acid concentration	1776:1828	a lower blood non-esterified fatty acid concentration than the AL group	1776:1846	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	3	71	from	parity	608:613	arg1	milk					624:627	milk	624:627	milk	624:627	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	1	72	theme	Optimising	163:172	arg1	feed					174:177	Optimising feed	163:177	Optimising feed	163:177	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	1	72	theme	Optimising	163:172	arg1	challenge					188:196	a key challenge	182:196	a key challenge for dairy livestock systems	182:224	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	10	73	theme	blood	1784:1788	arg1	concentration					1816:1828	a lower blood non-esterified fatty acid concentration	1776:1828	a lower blood non-esterified fatty acid concentration than the AL group	1776:1846	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	4	74	dep	concentrate	724:734	arg1	access					761:766	access	761:766	access	761:766	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	12	75	theme	Access-time	1957:1967	arg1	restriction					1969:1979	Access-time restriction	1957:1979	Access-time restriction	1957:1979	Access-time restriction did not impact fat and protein content but led to decreased casein, lactose contents and casein-to-whey protein ratio.
35963104	4	76	theme	first-cut	771:779	arg1	hay					781:783	first-cut hay	771:783	first-cut hay (Ad Libitum group; AL)	771:806	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	13	77	theme	management	2146:2155	arg1	practice					2157:2164	this feed management practice	2136:2164	this feed management practice	2136:2164	The forage savings achieved through this feed management practice could prove economically substantial when forage prices increase.
35963104	9	78	contain	had	1532:1534	arg2	content					1620:1626	milk protein content	1607:1626	milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk)	1607:1663	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	78	contain	had	1532:1534	arg2	fat					1562:1564	significantly higher milk fat	1536:1564	significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk)	1536:1601	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	78	contain	had	1532:1534	arg1	group					1526:1530	the AL group	1519:1530	the AL group	1519:1530	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	4	79	dep	libitum	753:759	arg1	i					747:747	i	747:747	i	747:747	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	13	80	theme	feed	2141:2144	arg1	practice					2157:2164	this feed management practice	2136:2164	this feed management practice	2136:2164	The forage savings achieved through this feed management practice could prove economically substantial when forage prices increase.
35963104	9	81	theme	milk	1659:1662	arg1	29.5					1629:1632	29.5	1629:1632	29.5	1629:1632	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	81	theme	milk	1659:1662	arg1	milk					1659:1662	milk	1659:1662	milk	1659:1662	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	1	82	theme	key	184:186	arg1	feed					174:177	Optimising feed	163:177	Optimising feed	163:177	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	1	82	theme	key	184:186	arg1	challenge					188:196	a key challenge	182:196	a key challenge for dairy livestock systems	182:224	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	14	83	theme	climatic	2306:2313	arg1	hazards					2315:2321	certain climatic hazards	2298:2321	certain climatic hazards	2298:2321	This practice can be of interest in grassland systems to overcome certain climatic hazards without having to resort to purchases or to increase the farm's forage autonomy.
35963104	3	84	from	yield	639:643	arg1	milk					624:627	milk	624:627	milk	624:627	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	11	85	theme	corrected	1912:1920	arg1	BW					1922:1923	their final corrected BW	1900:1923	their final corrected BW	1900:1923	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	0	86	theme	dairy	139:143	arg1	cows					145:148	dairy cows	139:148	dairy cows	139:148	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	4	87	dep	morning	963:969	arg1	the					959:961	the	959:961	the	959:961	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	88	from	2 h	952:954	arg1	morning					963:969	morning	963:969	morning	963:969	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	88	from	2 h	952:954	arg1	2 h					975:977	2 h	975:977	2 h	975:977	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	9	89	theme	QR	1500:1501	arg1	groups					1511:1516	the QR and QTR groups	1496:1516	the QR and QTR groups	1496:1516	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	13	90	dep	savings	2111:2117	arg1	forage					2104:2109	forage	2104:2109	forage	2104:2109	The forage savings achieved through this feed management practice could prove economically substantial when forage prices increase.
35963104	3	91	theme	balanced	589:596	arg1	cows					584:587	nine cows	579:587	nine cows balanced by breed, parity, days in milk, and milk yield	579:643	Twenty-seven Montbéliarde and Holstein cows were randomly allocated to three groups of nine cows balanced by breed, parity, days in milk, and milk yield.
35963104	7	92	theme	QTR	1366:1368	arg1	groups					1370:1375	the QR and QTR groups	1355:1375	the QR and QTR groups	1355:1375	Organic matter digestibility was lower in the AL group than in the QR and QTR groups whereas feed efficiency did not differ.
35963104	9	93	theme	QTR	1507:1509	arg1	groups					1511:1516	the QR and QTR groups	1496:1516	the QR and QTR groups	1496:1516	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	4	94	theme	Quantity-and-Time-restricted	997:1024	arg1	QTR					1033:1035	Quantity-and-Time-restricted group; QTR	997:1035	Quantity-and-Time-restricted group; QTR	997:1035	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	94	theme	Quantity-and-Time-restricted	997:1024	arg1	afternoon					986:994	the afternoon	982:994	the afternoon (Quantity-and-Time-restricted group; QTR)	982:1036	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	5	95	theme	condition	1128:1136	arg1	weight					1115:1120	weight	1115:1120	weight	1115:1120	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	5	95	theme	condition	1128:1136	arg1	score					1138:1142	body condition score	1123:1142	body condition score	1123:1142	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	9	96	theme	milk	1597:1600	arg1	35.9					1567:1570	35.9	1567:1570	35.9	1567:1570	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	9	96	theme	milk	1597:1600	arg1	milk					1597:1600	milk	1597:1600	milk	1597:1600	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	7	97	theme	QR	1359:1360	arg1	groups					1370:1375	the QR and QTR groups	1355:1375	the QR and QTR groups	1355:1375	Organic matter digestibility was lower in the AL group than in the QR and QTR groups whereas feed efficiency did not differ.
35963104	2	98	theme	nutritional	450:460	arg1	status					462:467	animal nutritional status	443:467	animal nutritional status	443:467	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	5	99	theme	nutritional	1095:1105	arg1	status					1107:1112	cow nutritional status	1091:1112	cow nutritional status (weight, body condition score, blood metabolites)	1091:1162	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	0	100	theme	feed	77:80	arg1	efficiency					82:91	feed efficiency	77:91	feed efficiency	77:91	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	1	101	theme	operating	303:311	arg1	cost					313:316	the biggest operating cost	291:316	the biggest operating cost	291:316	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	1	101	theme	operating	303:311	arg1	feed					283:286	feed	283:286	feed	283:286	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	9	102	theme	AL	1523:1524	arg1	group					1526:1530	the AL group	1519:1530	the AL group	1519:1530	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	1	103	dep	shortages	243:251	arg1	forage					230:235	forage	230:235	forage	230:235	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	10	104	theme	final	1729:1733	arg1	score					1750:1754	a lower final body condition score	1721:1754	a lower final body condition score	1721:1754	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	6	105	theme	AL	1202:1203	arg1	group					1205:1209	The AL group	1198:1209	The AL group	1198:1209	The AL group ingested 10 % more feed than the QR group and 16 % more feed than the QTR group.
35963104	4	106	theme	first-cut	828:836	arg1	QR					870:871	Quantity-restricted group; QR	843:871	Quantity-restricted group; QR	843:871	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	106	theme	first-cut	828:836	arg1	hay					838:840	first-cut hay	828:840	first-cut hay (Quantity-restricted group; QR)	828:872	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	107	theme	second-cut	691:700	arg1	hay					702:704	second-cut hay	691:704	second-cut hay	691:704	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	2	108	dep	quantity	393:400	arg1	forage					386:391	forage	386:391	forage	386:391	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	9	109	theme	higher	1550:1555	arg1	fat					1562:1564	significantly higher milk fat	1536:1564	significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk)	1536:1601	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	4	110	theme	Quantity-restricted	843:861	arg1	QR					870:871	Quantity-restricted group; QR	843:871	Quantity-restricted group; QR	843:871	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	110	theme	Quantity-restricted	843:861	arg1	hay					838:840	first-cut hay	828:840	first-cut hay (Quantity-restricted group; QR)	828:872	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	111	dep	hay	781:783	arg1	group					797:801	Ad Libitum group	786:801	Ad Libitum group	786:801	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	11	112	theme	QTR	1849:1851	arg1	cows					1853:1856	QTR cows	1849:1856	QTR cows	1849:1856	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	2	113	theme	experiment	335:344	arg1	aim					323:325	The aim	319:325	The aim of this experiment	319:344	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	0	114	theme	quantity	18:25	arg1	Effects					0:6	Effects	0:6	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows	0:148	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	4	115	theme	first-cut	898:906	arg1	hay					908:910	first-cut hay	898:910	first-cut hay	898:910	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	4	116	dep	group	797:801	arg1	AL					804:805	AL	804:805	AL	804:805	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	5	117	theme	Milk	1039:1042	arg1	yield					1044:1048	Milk yield	1039:1048	Milk yield	1039:1048	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35963104	7	118	theme	AL	1338:1339	arg1	group					1341:1345	the AL group	1334:1345	the AL group	1334:1345	Organic matter digestibility was lower in the AL group than in the QR and QTR groups whereas feed efficiency did not differ.
35963104	10	119	theme	condition	1740:1748	arg1	score					1750:1754	a lower final body condition score	1721:1754	a lower final body condition score	1721:1754	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	2	120	theme	dairy	421:425	arg1	performance					427:437	dairy performance	421:437	dairy performance	421:437	The aim of this experiment was to evaluate the effects of reducing forage quantity and access time on dairy performance and animal nutritional status during indoor feeding.
35963104	12	121	theme	casein-to-whey	2070:2083	arg1	ratio					2093:2097	casein-to-whey protein ratio	2070:2097	casein-to-whey protein ratio	2070:2097	Access-time restriction did not impact fat and protein content but led to decreased casein, lactose contents and casein-to-whey protein ratio.
35963104	11	122	theme	final	1906:1910	arg1	BW					1922:1923	their final corrected BW	1900:1923	their final corrected BW	1900:1923	QTR cows showed greater body fat mobilisation, but their final corrected BW was not different from AL cows.
35963104	6	123	theme	feed	1230:1233	arg1	%					1223:1223	10 %	1220:1223	10 % more feed than the QR group	1220:1251	The AL group ingested 10 % more feed than the QR group and 16 % more feed than the QTR group.
35963104	4	124	theme	ad	750:751	arg1	libitum					753:759	either i) ad libitum	740:759	either i) ad libitum	740:759	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	0	125	dep	quantity	18:25	arg1	forage					11:16	forage	11:16	forage	11:16	Effects of forage quantity and access-time restriction on feeding behaviour, feed efficiency, nutritional status, and dairy performance of dairy cows fed indoors.
35963104	4	126	theme	restricted	933:942	arg1	time					928:931	access time	921:931	access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR)	921:1036	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	10	127	contain	have	1771:1774	arg2	concentration					1816:1828	a lower blood non-esterified fatty acid concentration	1776:1828	a lower blood non-esterified fatty acid concentration than the AL group	1776:1846	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	10	127	contain	have	1771:1774	arg1	QR					1666:1667	QR	1666:1667	QR	1666:1667	QR and QTR cows mobilised their body fat, resulting in a lower final body condition score, and tended to have a lower blood non-esterified fatty acid concentration than the AL group.
35963104	6	128	theme	QTR	1281:1283	arg1	group					1285:1289	the QTR group	1277:1289	the QTR group	1277:1289	The AL group ingested 10 % more feed than the QR group and 16 % more feed than the QTR group.
35963104	1	129	theme	dairy	202:206	arg1	systems					218:224	dairy livestock systems	202:224	dairy livestock systems	202:224	Optimising feed is a key challenge for dairy livestock systems, as forage stock shortages are increasingly frequent and feed is the biggest operating cost.
35963104	7	130	theme	matter	1300:1305	arg1	digestibility					1307:1319	Organic matter digestibility	1292:1319	Organic matter digestibility	1292:1319	Organic matter digestibility was lower in the AL group than in the QR and QTR groups whereas feed efficiency did not differ.
35963104	4	131	theme	only	947:950	arg1	2 h					952:954	only 2 h	947:954	only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR)	947:1036	The three groups were given 3.9 kg DM/day of second-cut hay and 4.5 kg/day of concentrate and either i) ad libitum access to first-cut hay (Ad Libitum group; AL), ii) 10.5 kg/day of first-cut hay (Quantity-restricted group; QR), or iii) 10.5 kg/day of first-cut hay but with access time restricted to only 2 h in the morning and 2 h in the afternoon (Quantity-and-Time-restricted group; QTR).
35963104	14	132	from	interest	2256:2263	arg1	systems					2278:2284	grassland systems	2268:2284	grassland systems	2268:2284	This practice can be of interest in grassland systems to overcome certain climatic hazards without having to resort to purchases or to increase the farm's forage autonomy.
35963104	9	133	theme	milk	1557:1560	arg1	fat					1562:1564	significantly higher milk fat	1536:1564	significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk)	1536:1601	Compared to the QR and QTR groups, the AL group had significantly higher milk fat (35.9 vs 32.9 and 32.8 g/kg of milk) and milk protein content (29.5 vs 27.7 and 28.5 g/kg of milk).
35963104	5	134	theme	cow	1091:1093	arg1	status					1107:1112	cow nutritional status	1091:1112	cow nutritional status (weight, body condition score, blood metabolites)	1091:1162	Milk yield, composition and coagulation properties, cow nutritional status (weight, body condition score, blood metabolites) and cow activities were recorded.
35033088	4	0	theme	factorial	830:838	arg1	arrangement					840:850	a 2 × 2 factorial arrangement	822:850	a 2 × 2 factorial arrangement	822:850	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	10	1	theme	serum	2203:2207	arg1	LPS					2209:2211	serum LPS	2203:2211	serum LPS	2203:2211	Moreover, Bacteroidetes was positively correlated with serum LPS.
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	IgA					1192:1194	IgA	1192:1194	IgA	1192:1194	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	IL-1β					1227:1231	IL-1β	1227:1231	IL-1β	1227:1231	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	butyrate					1321:1328	butyrate	1321:1328	butyrate	1321:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	acetate					1308:1314	acetate	1308:1314	acetate	1308:1314	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	contents					1180:1187	contents	1180:1187	contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum	1180:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	amounts					1262:1268	the amounts	1258:1268	the amounts of total volatile fatty acids (TVFA), acetate, and butyrate	1258:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	interleukin-1β					1211:1224	interleukin-1β	1211:1224	interleukin-1β (IL-1β)	1211:1232	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	2	theme	interleukin-1β	1211:1224	arg1	IL-6					1239:1242	IL-6	1239:1242	IL-6	1239:1242	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	7	3	from	concentration	1550:1562	arg1	rumen					1571:1575	the rumen	1567:1575	the rumen of beef cattle	1567:1590	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	4	4	theme	transport	576:584	arg1	effects					565:571	the effects	561:571	the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle	561:698	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	6	5	theme	γ	1208:1208	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	IgA					1192:1194	IgA	1192:1194	IgA	1192:1194	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	IL-1β					1227:1231	IL-1β	1227:1231	IL-1β	1227:1231	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	butyrate					1321:1328	butyrate	1321:1328	butyrate	1321:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	acetate					1308:1314	acetate	1308:1314	acetate	1308:1314	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	contents					1180:1187	contents	1180:1187	contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum	1180:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	amounts					1262:1268	the amounts	1258:1268	the amounts of total volatile fatty acids (TVFA), acetate, and butyrate	1258:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	interleukin-1β					1211:1224	interleukin-1β	1211:1224	interleukin-1β (IL-1β)	1211:1232	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	5	theme	γ	1208:1208	arg1	IL-6					1239:1242	IL-6	1239:1242	IL-6	1239:1242	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	3	6	theme	energy	527:532	arg1	metabolism					534:543	energy metabolism	527:543	energy metabolism	527:543	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	4	7	theme	biochemical	630:640	arg1	parameters					642:651	the blood biochemical parameters	620:651	the blood biochemical parameters	620:651	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	1	8	theme	road	187:190	arg1	transportation					192:205	road transportation	187:205	road transportation	187:205	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	8	9	theme	serum	1732:1736	arg1	factor-α					1754:1761	serum tumour necrosis factor-α	1732:1761	serum tumour necrosis factor-α	1732:1761	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	8	10	theme	significant	1694:1704	arg1	effect					1706:1711	a significant effect	1692:1711	a significant effect	1692:1711	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	4	11	theme	-CrPyr	610:615	arg1	effects					565:571	the effects	561:571	the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle	561:698	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	8	12	theme	necrosis	1745:1752	arg1	factor-α					1754:1761	serum tumour necrosis factor-α	1732:1761	serum tumour necrosis factor-α	1732:1761	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	6	13	theme	lipopolysaccharide	1371:1388	arg1	level					1396:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	4	14	dep	groups	801:806	arg1	NT_CrPyr0					990:998	NT_CrPyr0	990:998	NT_CrPyr0	990:998	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	14	dep	groups	801:806	arg1	T_CrPyr0					963:970	T_CrPyr0	963:970	T_CrPyr0	963:970	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	14	dep	groups	801:806	arg1	groups					801:806	four groups	796:806	four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0	796:998	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	14	dep	groups	801:806	arg1	NT_CrPyr140					973:983	NT_CrPyr140	973:983	NT_CrPyr140	973:983	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	6	15	theme	IL-6	1239:1242	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	IgA					1192:1194	IgA	1192:1194	IgA	1192:1194	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	IL-1β					1227:1231	IL-1β	1227:1231	IL-1β	1227:1231	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	butyrate					1321:1328	butyrate	1321:1328	butyrate	1321:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	acetate					1308:1314	acetate	1308:1314	acetate	1308:1314	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	contents					1180:1187	contents	1180:1187	contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum	1180:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	amounts					1262:1268	the amounts	1258:1268	the amounts of total volatile fatty acids (TVFA), acetate, and butyrate	1258:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	interleukin-1β					1211:1224	interleukin-1β	1211:1224	interleukin-1β (IL-1β)	1211:1232	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	15	theme	IL-6	1239:1242	arg1	IL-6					1239:1242	IL-6	1239:1242	IL-6	1239:1242	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	1	16	from	lack	224:227	arg1	system					267:272	the immune system	256:272	the immune system of beef cattle	256:287	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	4	17	theme	RP-CrPyr	861:868	arg1	0					891:891	0	891:891	0	891:891	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	17	theme	RP-CrPyr	861:868	arg1	levels					883:888	two RP-CrPyr supplemental levels	857:888	two RP-CrPyr supplemental levels (0 or 140 g/d)	857:903	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	17	theme	RP-CrPyr	861:868	arg1	140 g/d					896:902	140 g/d	896:902	140 g/d	896:902	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	0	18	from	parameters	66:75	arg1	cattle					129:134	transported beef cattle	112:134	transported beef cattle	112:134	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	4	19	theme	cattle	693:698	arg1	characteristics					669:683	rumen fluid characteristics	657:683	rumen fluid characteristics	657:683	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	19	theme	cattle	693:698	arg1	parameters					642:651	the blood biochemical parameters	620:651	the blood biochemical parameters	620:651	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	9	20	theme	phylum	2024:2029	arg1	abundance					2031:2039	Firmicutes phylum abundance	2013:2039	Firmicutes phylum abundance	2013:2039	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	1	21	from	decline	245:251	arg1	system					267:272	the immune system	256:272	the immune system of beef cattle	256:287	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	7	22	theme	cattle	1585:1590	arg1	rumen					1571:1575	the rumen	1567:1575	the rumen of beef cattle	1567:1590	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	11	23	theme	rumen	2424:2428	arg1	flora					2438:2442	the rumen natural flora	2420:2442	the rumen natural flora	2420:2442	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	7	24	from	serum	1527:1531	arg1	rumen					1571:1575	the rumen	1567:1575	the rumen of beef cattle	1567:1590	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	4	25	dep	treatments	923:932	arg1	12 h					944:947	12 h	944:947	12 h	944:947	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	25	dep	treatments	923:932	arg1	5 min					935:939	5 min	935:939	5 min	935:939	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	25	dep	treatments	923:932	arg1	treatments					923:932	two transport treatments	909:932	two transport treatments (5 min or 12 h)	909:948	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	8	26	theme	acetate	1787:1793	arg1	content					1795:1801	acetate content	1787:1801	acetate content	1787:1801	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	0	27	from	characteristics	93:107	arg1	cattle					129:134	transported beef cattle	112:134	transported beef cattle	112:134	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	6	28	theme	antioxidant	1139:1149	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	4	29	theme	fluid	663:667	arg1	characteristics					669:683	rumen fluid characteristics	657:683	rumen fluid characteristics	657:683	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	9	30	theme	Bacteroidetes	2055:2067	arg1	abundance					2097:2105	Bacteroidetes phylum and genus Prevotella abundance	2055:2105	Bacteroidetes phylum and genus Prevotella abundance	2055:2105	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	6	31	theme	fatty	1288:1292	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	31	theme	fatty	1288:1292	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	IgA					1192:1194	IgA	1192:1194	IgA	1192:1194	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	IL-1β					1227:1231	IL-1β	1227:1231	IL-1β	1227:1231	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	butyrate					1321:1328	butyrate	1321:1328	butyrate	1321:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	acetate					1308:1314	acetate	1308:1314	acetate	1308:1314	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	contents					1180:1187	contents	1180:1187	contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum	1180:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	amounts					1262:1268	the amounts	1258:1268	the amounts of total volatile fatty acids (TVFA), acetate, and butyrate	1258:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	interleukin-1β					1211:1224	interleukin-1β	1211:1224	interleukin-1β (IL-1β)	1211:1232	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	32	theme	acetate	1308:1314	arg1	IL-6					1239:1242	IL-6	1239:1242	IL-6	1239:1242	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	33	theme	rumen	1415:1419	arg1	LPS					1421:1423	rumen LPS	1415:1423	rumen LPS	1415:1423	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	0	34	theme	rumen	81:85	arg1	characteristics					93:107	rumen fluid characteristics	81:107	rumen fluid characteristics	81:107	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	0	35	from	Effects	0:6	arg1	parameters					66:75	blood biochemical parameters	48:75	blood biochemical parameters	48:75	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	0	35	from	Effects	0:6	arg1	characteristics					93:107	rumen fluid characteristics	81:107	rumen fluid characteristics	81:107	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	1	36	dep	BACKGROUND	137:146	arg1	contributes					207:217	contributes	207:217	contributes to a lack of energy and a decline in the immune system of beef cattle	207:287	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	6	37	from	capacity	1151:1158	arg1	serum					1247:1251	serum	1247:1251	serum	1247:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	8	38	theme	tumour	1738:1743	arg1	factor-α					1754:1761	serum tumour necrosis factor-α	1732:1761	serum tumour necrosis factor-α	1732:1761	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	4	39	theme	transport	913:921	arg1	12 h					944:947	12 h	944:947	12 h	944:947	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	39	theme	transport	913:921	arg1	5 min					935:939	5 min	935:939	5 min	935:939	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	39	theme	transport	913:921	arg1	treatments					923:932	two transport treatments	909:932	two transport treatments (5 min or 12 h)	909:948	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	11	40	theme	LPS	2376:2378	arg1	levels					2380:2385	serum cortisol and LPS levels	2357:2385	levels	2380:2385	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	4	41	theme	aged	754:757	arg1	18 months					759:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	11	42	dep	CONCLUSIONS	2214:2224	arg1	indicated					2240:2248	indicated	2240:2248	indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora	2240:2442	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	3	43	theme	new	414:416	arg1	pyruvate					392:399	Creatine pyruvate	383:399	Creatine pyruvate (CrPyr)	383:407	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	43	theme	new	414:416	arg1	nutrient					434:441	a new multifunctional nutrient	412:441	a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism	412:543	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	0	44	theme	transported	112:122	arg1	cattle					129:134	transported beef cattle	112:134	transported beef cattle	112:134	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	11	45	theme	dietary	2255:2261	arg1	supplementation					2263:2277	dietary supplementation	2255:2277	dietary supplementation with RP-CrPyr	2255:2291	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	4	46	theme	Simmental	713:721	arg1	18 months					759:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	9	47	theme	group	2121:2125	arg1	P < 0.05					2137:2144	P < 0.05	2137:2144	P < 0.05	2137:2144	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	9	47	theme	group	2121:2125	arg1	T_CrPyr0					2127:2134	group T_CrPyr0	2121:2134	group T_CrPyr0 (P < 0.05)	2121:2145	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	7	48	theme	LPS	1520:1522	arg1	levels					1497:1502	the levels	1493:1502	the levels of cortisol and LPS	1493:1522	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	9	49	theme	bacterial	1859:1867	arg1	composition					1869:1879	ruminal bacterial composition	1851:1879	ruminal bacterial composition	1851:1879	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	1	50	dep	fasting	152:158	arg1	The					148:150	The	148:150	The	148:150	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	0	51	theme	rumen-protected	11:25	arg1	pyruvate					36:43	rumen-protected creatine pyruvate	11:43	rumen-protected creatine pyruvate	11:43	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	1	52	theme	cattle	282:287	arg1	system					267:272	the immune system	256:272	the immune system of beef cattle	256:287	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	9	53	dep	Bacteroidetes	2055:2067	arg1	phylum					2069:2074	phylum	2069:2074	phylum	2069:2074	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	7	54	theme	cortisol	1507:1514	arg1	levels					1497:1502	the levels	1493:1502	the levels of cortisol and LPS	1493:1522	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	0	55	theme	pyruvate	36:43	arg1	Effects					0:6	Effects	0:6	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.	0:135	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	8	56	theme	pH	1783:1784	arg1	contents					1720:1727	the contents	1716:1727	the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05)	1716:1836	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	0	57	theme	blood	48:52	arg1	parameters					66:75	blood biochemical parameters	48:75	blood biochemical parameters	48:75	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	6	58	theme	increased	1351:1359	arg1	level					1396:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	8	59	contain	had	1688:1690	arg1	RP-CrPyr					1653:1660	RP-CrPyr	1653:1660	RP-CrPyr	1653:1660	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	8	59	contain	had	1688:1690	arg1	interaction					1676:1686	transport interaction	1666:1686	transport interaction	1666:1686	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	8	59	contain	had	1688:1690	arg2	effect					1706:1711	a significant effect	1692:1711	a significant effect	1692:1711	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	4	60	theme	rumen	657:661	arg1	characteristics					669:683	rumen fluid characteristics	657:683	rumen fluid characteristics	657:683	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	9	61	theme	genus	1922:1926	arg1	abundance					1928:1936	the Prevotella genus abundance	1907:1936	the Prevotella genus abundance	1907:1936	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	11	62	theme	flora	2438:2442	arg1	restoration					2405:2415	the restoration	2401:2415	the restoration of the rumen natural flora	2401:2442	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	6	63	theme	volatile	1279:1286	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	63	theme	volatile	1279:1286	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	4	64	theme	2 × 2	824:828	arg1	arrangement					840:850	a 2 × 2 factorial arrangement	822:850	a 2 × 2 factorial arrangement	822:850	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	1	65	theme	immune	260:265	arg1	system					267:272	the immune system	256:272	the immune system of beef cattle	256:287	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	7	66	theme	unsupplemented	1619:1632	arg1	groups					1634:1639	the unsupplemented groups	1615:1639	the unsupplemented groups (P < 0.05)	1615:1650	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	7	66	theme	unsupplemented	1619:1632	arg1	P < 0.05					1642:1649	P < 0.05	1642:1649	P < 0.05	1642:1649	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	6	67	theme	interferon	1197:1206	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	3	68	theme	intermediate	502:513	arg1	creatine					478:485	creatine	478:485	creatine	478:485	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	68	theme	intermediate	502:513	arg1	products					515:522	two intermediate products	498:522	two intermediate products of energy metabolism	498:543	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	68	theme	intermediate	502:513	arg1	pyruvate					465:472	pyruvate	465:472	pyruvate	465:472	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	4	69	theme	blood	624:628	arg1	parameters					642:651	the blood biochemical parameters	620:651	the blood biochemical parameters	620:651	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	6	70	theme	total	1273:1277	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	70	theme	total	1273:1277	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	IgA					1192:1194	IgA	1192:1194	IgA	1192:1194	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	IL-1β					1227:1231	IL-1β	1227:1231	IL-1β	1227:1231	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	butyrate					1321:1328	butyrate	1321:1328	butyrate	1321:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	acetate					1308:1314	acetate	1308:1314	acetate	1308:1314	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	contents					1180:1187	contents	1180:1187	contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum	1180:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	amounts					1262:1268	the amounts	1258:1268	the amounts of total volatile fatty acids (TVFA), acetate, and butyrate	1258:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	interleukin-1β					1211:1224	interleukin-1β	1211:1224	interleukin-1β (IL-1β)	1211:1232	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	71	theme	IgA	1192:1194	arg1	IL-6					1239:1242	IL-6	1239:1242	IL-6	1239:1242	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	8	72	theme	factor-α	1754:1761	arg1	contents					1720:1727	the contents	1716:1727	the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05)	1716:1836	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	3	73	theme	metabolism	534:543	arg1	creatine					478:485	creatine	478:485	creatine	478:485	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	73	theme	metabolism	534:543	arg1	products					515:522	two intermediate products	498:522	two intermediate products of energy metabolism	498:543	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	73	theme	metabolism	534:543	arg1	pyruvate					465:472	pyruvate	465:472	pyruvate	465:472	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	8	74	theme	IL-6	1764:1767	arg1	contents					1720:1727	the contents	1716:1727	the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05)	1716:1836	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	6	75	theme	serum	1365:1369	arg1	LPS					1391:1393	LPS	1391:1393	LPS	1391:1393	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	75	theme	serum	1365:1369	arg1	lipopolysaccharide					1371:1388	the serum lipopolysaccharide	1361:1388	increased the serum lipopolysaccharide (LPS) level	1351:1400	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	7	76	theme	RP-CrPyr	1458:1465	arg1	supplementation					1467:1481	RP-CrPyr supplementation	1458:1481	RP-CrPyr supplementation	1458:1481	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	8	77	theme	LPS	1770:1772	arg1	contents					1720:1727	the contents	1716:1727	the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05)	1716:1836	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	6	78	theme	catalase	1161:1168	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	4	79	theme	beef	688:691	arg1	cattle					693:698	beef cattle	688:698	beef cattle	688:698	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	6	80	from	activity	1170:1177	arg1	serum					1247:1251	serum	1247:1251	serum	1247:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	8	81	theme	ruminal	1775:1781	arg1	pH					1783:1784	ruminal pH	1775:1784	ruminal pH	1775:1784	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	6	82	theme	ammonia	1429:1435	arg1	nitrogen					1437:1444	ammonia nitrogen	1429:1444	ammonia nitrogen (P < 0.05)	1429:1455	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	82	theme	ammonia	1429:1435	arg1	P < 0.05					1447:1454	P < 0.05	1447:1454	P < 0.05	1447:1454	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	4	83	theme	supplemental	870:881	arg1	0					891:891	0	891:891	0	891:891	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	83	theme	supplemental	870:881	arg1	levels					883:888	two RP-CrPyr supplemental levels	857:888	two RP-CrPyr supplemental levels (0 or 140 g/d)	857:903	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	83	theme	supplemental	870:881	arg1	140 g/d					896:902	140 g/d	896:902	140 g/d	896:902	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	6	84	from	contents	1180:1187	arg1	serum					1247:1251	serum	1247:1251	serum	1247:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	IgA					1192:1194	IgA	1192:1194	IgA	1192:1194	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	IL-1β					1227:1231	IL-1β	1227:1231	IL-1β	1227:1231	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	butyrate					1321:1328	butyrate	1321:1328	butyrate	1321:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	acetate					1308:1314	acetate	1308:1314	acetate	1308:1314	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	contents					1180:1187	contents	1180:1187	contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum	1180:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	amounts					1262:1268	the amounts	1258:1268	the amounts of total volatile fatty acids (TVFA), acetate, and butyrate	1258:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	interleukin-1β					1211:1224	interleukin-1β	1211:1224	interleukin-1β (IL-1β)	1211:1232	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	85	theme	acids	1294:1298	arg1	IL-6					1239:1242	IL-6	1239:1242	IL-6	1239:1242	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	86	theme	LPS	1421:1423	arg1	level					1396:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	86	theme	LPS	1421:1423	arg1	contents					1403:1410	contents	1403:1410	contents of rumen LPS	1403:1423	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	86	theme	LPS	1421:1423	arg1	P < 0.05					1447:1454	P < 0.05	1447:1454	P < 0.05	1447:1454	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	86	theme	LPS	1421:1423	arg1	nitrogen					1437:1444	ammonia nitrogen	1429:1444	ammonia nitrogen (P < 0.05)	1429:1455	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	7	87	theme	beef	1580:1583	arg1	cattle					1585:1590	beef cattle	1580:1590	beef cattle	1580:1590	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	6	88	theme	total	1133:1137	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	1	89	theme	energy	232:237	arg1	decline					245:251	a decline	243:251	a decline in the immune system of beef cattle	243:287	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	1	89	theme	energy	232:237	arg1	lack					224:227	a lack	222:227	a lack of energy	222:237	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	0	90	theme	fluid	87:91	arg1	characteristics					93:107	rumen fluid characteristics	81:107	rumen fluid characteristics	81:107	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	6	91	dep	Compared	1081:1088	arg1	nitrogen					1437:1444	ammonia nitrogen	1429:1444	ammonia nitrogen (P < 0.05)	1429:1455	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	91	dep	Compared	1081:1088	arg1	level					1396:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	increased the serum lipopolysaccharide (LPS) level	1351:1400	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	91	dep	Compared	1081:1088	arg1	P < 0.05					1447:1454	P < 0.05	1447:1454	P < 0.05	1447:1454	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	91	dep	Compared	1081:1088	arg1	contents					1403:1410	contents	1403:1410	contents of rumen LPS	1403:1423	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	11	92	with	supplementation	2263:2277	arg1	RP-CrPyr					2284:2291	RP-CrPyr	2284:2291	RP-CrPyr	2284:2291	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	11	93	theme	transport	2326:2334	arg1	stress					2336:2341	transport stress	2326:2341	transport stress	2326:2341	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	9	94	theme	genus	2080:2084	arg1	abundance					2097:2105	Bacteroidetes phylum and genus Prevotella abundance	2055:2105	Bacteroidetes phylum and genus Prevotella abundance	2055:2105	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	0	95	theme	beef	124:127	arg1	cattle					129:134	transported beef cattle	112:134	transported beef cattle	112:134	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	7	96	theme	butyrate	1541:1548	arg1	concentration					1550:1562	the butyrate concentration	1537:1562	the butyrate concentration in the rumen of beef cattle	1537:1590	RP-CrPyr supplementation decreased the levels of cortisol and LPS in serum and the butyrate concentration in the rumen of beef cattle compared with those in the unsupplemented groups (P < 0.05).
35033088	11	97	theme	serum	2357:2361	arg1	cortisol					2363:2370	serum cortisol and LPS levels	2357:2385	cortisol	2363:2370	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	4	98	from	effects	565:571	arg1	characteristics					669:683	rumen fluid characteristics	657:683	rumen fluid characteristics	657:683	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	4	98	from	effects	565:571	arg1	parameters					642:651	the blood biochemical parameters	620:651	the blood biochemical parameters	620:651	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	6	99	theme	butyrate	1321:1328	arg1	capacity					1151:1158	the total antioxidant capacity	1129:1158	the total antioxidant capacity	1129:1158	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	IgA					1192:1194	IgA	1192:1194	IgA	1192:1194	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	IL-1β					1227:1231	IL-1β	1227:1231	IL-1β	1227:1231	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	butyrate					1321:1328	butyrate	1321:1328	butyrate	1321:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	acetate					1308:1314	acetate	1308:1314	acetate	1308:1314	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	acids					1294:1298	total volatile fatty acids	1273:1298	total volatile fatty acids (TVFA)	1273:1305	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	activity					1170:1177	catalase activity	1161:1177	catalase activity	1161:1177	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	γ					1208:1208	interferon γ	1197:1208	interferon γ	1197:1208	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	contents					1180:1187	contents	1180:1187	contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum	1180:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	amounts					1262:1268	the amounts	1258:1268	the amounts of total volatile fatty acids (TVFA), acetate, and butyrate	1258:1328	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	interleukin-1β					1211:1224	interleukin-1β	1211:1224	interleukin-1β (IL-1β)	1211:1232	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	TVFA					1301:1304	TVFA	1301:1304	TVFA	1301:1304	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	99	theme	butyrate	1321:1328	arg1	IL-6					1239:1242	IL-6	1239:1242	IL-6	1239:1242	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	6	100	from	amounts	1262:1268	arg1	serum					1247:1251	serum	1247:1251	serum	1247:1251	RESULTS Compared with nontransport, transport decreased the total antioxidant capacity, catalase activity, contents of IgA, interferon γ, interleukin-1β (IL-1β), and IL-6 in serum, and the amounts of total volatile fatty acids (TVFA), acetate, and butyrate in rumen (P < 0.05); increased the serum lipopolysaccharide (LPS) level, contents of rumen LPS and ammonia nitrogen (P < 0.05).
35033088	8	101	theme	transport	1666:1674	arg1	interaction					1676:1686	transport interaction	1666:1686	transport interaction	1666:1686	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	3	102	theme	Creatine	383:390	arg1	CrPyr					402:406	CrPyr	402:406	CrPyr	402:406	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	102	theme	Creatine	383:390	arg1	nutrient					434:441	a new multifunctional nutrient	412:441	a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism	412:543	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	102	theme	Creatine	383:390	arg1	pyruvate					392:399	Creatine pyruvate	383:399	Creatine pyruvate (CrPyr)	383:407	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	9	103	theme	ruminal	1851:1857	arg1	composition					1869:1879	ruminal bacterial composition	1851:1879	ruminal bacterial composition	1851:1879	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	4	104	theme	male	708:711	arg1	18 months					759:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	0	105	theme	creatine	27:34	arg1	pyruvate					36:43	rumen-protected creatine pyruvate	11:43	rumen-protected creatine pyruvate	11:43	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	1	106	theme	beef	277:280	arg1	cattle					282:287	beef cattle	277:287	beef cattle	277:287	BACKGROUND The fasting and stress associated with road transportation contributes to a lack of energy and a decline in the immune system of beef cattle.
35033088	9	107	theme	composition	1869:1879	arg1	terms					1842:1846	terms	1842:1846	terms of ruminal bacterial composition	1842:1879	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	4	108	theme	crossbred	723:731	arg1	18 months					759:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months	701:767	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	9	109	theme	group	1882:1886	arg1	T_CrPyr0					1888:1895	group T_CrPyr0	1882:1895	group T_CrPyr0	1882:1895	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	11	110	theme	natural	2430:2436	arg1	flora					2438:2442	the rumen natural flora	2420:2442	the rumen natural flora	2420:2442	CONCLUSIONS These results indicated that dietary supplementation with RP-CrPyr might be beneficial to alleviate transport stress by decreasing serum cortisol and LPS levels and promoting the restoration of the rumen natural flora.
35033088	8	111	theme	content	1795:1801	arg1	contents					1720:1727	the contents	1716:1727	the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05)	1716:1836	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	8	112	theme	acetate/propionate	1808:1825	arg1	contents					1720:1727	the contents	1716:1727	the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05)	1716:1836	RP-CrPyr and transport interaction had a significant effect on the contents of serum tumour necrosis factor-α, IL-6, LPS, ruminal pH, acetate content, and acetate/propionate (P < 0.05).
35033088	0	113	theme	biochemical	54:64	arg1	parameters					66:75	blood biochemical parameters	48:75	blood biochemical parameters	48:75	Effects of rumen-protected creatine pyruvate on blood biochemical parameters and rumen fluid characteristics in transported beef cattle.
35033088	2	114	theme	beef	321:324	arg1	cattle					326:331	beef cattle	321:331	beef cattle	321:331	Therefore, it is essential for beef cattle to enhance energy reserves before transportation.
35033088	4	115	theme	rumen-protected	590:604	arg1	-CrPyr					610:615	rumen-protected (RP)-CrPyr	590:615	rumen-protected (RP)-CrPyr	590:615	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	9	116	theme	Prevotella	1911:1920	arg1	abundance					1928:1936	the Prevotella genus abundance	1907:1936	the Prevotella genus abundance	1907:1936	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	9	117	dep	genus	2080:2084	arg1	Prevotella					2086:2095	Prevotella	2086:2095	Prevotella	2086:2095	In terms of ruminal bacterial composition, group T_CrPyr0 increased the Prevotella genus abundance compared with group NT_CrPyr0 (P < 0.05), while group T_CrPyr140 increased Firmicutes phylum abundance and decreased Bacteroidetes phylum and genus Prevotella abundance compared with group T_CrPyr0 (P < 0.05).
35033088	3	118	theme	multifunctional	418:432	arg1	pyruvate					392:399	Creatine pyruvate	383:399	Creatine pyruvate (CrPyr)	383:407	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	3	118	theme	multifunctional	418:432	arg1	nutrient					434:441	a new multifunctional nutrient	412:441	a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism	412:543	Creatine pyruvate (CrPyr) is a new multifunctional nutrient that can provide both pyruvate and creatine, which are two intermediate products of energy metabolism.
35033088	4	119	theme	RP	607:608	arg1	-CrPyr					610:615	rumen-protected (RP)-CrPyr	590:615	rumen-protected (RP)-CrPyr	590:615	To investigate the effects of transport and rumen-protected (RP)-CrPyr on the blood biochemical parameters and rumen fluid characteristics of beef cattle, twenty male Simmental crossbred cattle (659 ± 16 kg) aged 18 months were randomly allocated to four groups (n = 5) using a 2 × 2 factorial arrangement with two RP-CrPyr supplemental levels (0 or 140 g/d) and two transport treatments (5 min or 12 h): T_CrPyr140, T_CrPyr0, NT_CrPyr140, and NT_CrPyr0.
35033088	2	120	theme	energy	344:349	arg1	reserves					351:358	energy reserves	344:358	energy reserves	344:358	Therefore, it is essential for beef cattle to enhance energy reserves before transportation.
36662401	6	0	theme	DCFDA	905:909	arg1	analysis					911:918	DCFDA analysis	905:918	DCFDA analysis	905:918	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	9	1	theme	exclusive	1683:1691	arg1	composition					1693:1703	the exclusive composition	1679:1703	the exclusive composition of gymnemic acid derivatives	1679:1732	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	6	2	theme	LPS	1082:1084	arg1	control					1086:1092	LPS control	1082:1092	LPS control	1082:1092	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	1	3	theme	COVID-19	250:257	arg1	patients					259:266	COVID-19 patients	250:266	COVID-19 patients	250:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	9	4	theme	acid	1717:1720	arg1	derivatives					1722:1732	gymnemic acid derivatives	1708:1732	gymnemic acid derivatives	1708:1732	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	4	5	theme	in	644:645	arg1	models					665:670	in vitro and in vivo models	644:670	in vitro and in vivo models	644:670	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	5	6	theme	long	856:859	arg1	acids					867:871	long fatty acids	856:871	long fatty acids	856:871	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	0	7	theme	acute	81:85	arg1	syndrome					108:115	acute respiratory distress syndrome	81:115	acute respiratory distress syndrome in rats	81:123	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	4	8	from	ARDS	636:639	arg1	models					665:670	in vitro and in vivo models	644:670	in vitro and in vivo models	644:670	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	6	9	theme	inflammatory	960:971	arg1	cytokine/chemokine					973:990	inflammatory cytokine/chemokine	960:990	inflammatory cytokine/chemokine	960:990	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	9	10	theme	anti-inflammatory	1748:1764	arg1	effects					1766:1772	better anti-inflammatory effects	1741:1772	better anti-inflammatory effects (inhibition of IL-6 and IL-1β)	1741:1803	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	7	11	theme	injury	1397:1402	arg1	elevation					1292:1300	the LPS-induced elevation	1276:1300	the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury	1276:1402	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	4	12	from	injury	625:630	arg1	models					665:670	in vitro and in vivo models	644:670	in vitro and in vivo models	644:670	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	9	13	contain	has	1675:1677	arg1	fraction					1658:1665	fraction 6	1658:1667	fraction 6 which has the exclusive composition of gymnemic acid derivatives	1658:1732	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	9	13	contain	has	1675:1677	arg2	composition					1693:1703	the exclusive composition	1679:1703	the exclusive composition of gymnemic acid derivatives	1679:1732	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	0	14	theme	NF-κB/MAPK	144:153	arg1	pathway					155:161	the NF-κB/MAPK pathway	140:161	the NF-κB/MAPK pathway	140:161	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	5	15	dep	HAEGS	726:730	arg1	the					722:724	the	722:724	the	722:724	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	7	16	theme	levels	1380:1385	arg1	elevation					1292:1300	the LPS-induced elevation	1276:1300	the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury	1276:1402	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	1	17	from	mortality	237:245	arg1	patients					259:266	COVID-19 patients	250:266	COVID-19 patients	250:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	1	18	from	causes	227:232	arg1	patients					259:266	COVID-19 patients	250:266	COVID-19 patients	250:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	10	19	theme	lung	1899:1902	arg1	injury					1904:1909	LPS-induced lung injury	1887:1909	LPS-induced lung injury	1887:1909	Overall, HAEGS significantly mitigated LPS-induced lung injury and ARDS by targeting the NF-κB/MAPK signalling pathway.
36662401	6	20	theme	reactive	1018:1025	arg1	ROS					1043:1045	ROS	1043:1045	ROS	1043:1045	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	6	20	theme	reactive	1018:1025	arg1	species					1034:1040	reactive oxygen species	1018:1040	reactive oxygen species (ROS)	1018:1046	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	0	21	from	syndrome	108:115	arg1	rats					120:123	rats	120:123	rats	120:123	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	7	22	theme	marker	1357:1362	arg1	expression					1364:1373	cytokine/chemokine marker expression	1338:1373	cytokine/chemokine marker expression	1338:1373	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	7	23	theme	ARDS	1214:1217	arg1	model					1219:1223	LPS-induced ARDS model	1202:1223	LPS-induced ARDS model	1202:1223	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	11	24	theme	first	2029:2033	arg1	time					2035:2038	the first time	2025:2038	the first time in managing ARDS	2025:2055	Thus, our work unravels the protective role of HAEGS for the first time in managing ARDS.
36662401	1	25	theme	Acute	164:168	arg1	ARDS					201:204	ARDS	201:204	ARDS	201:204	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	1	25	theme	Acute	164:168	arg1	syndrome					191:198	Acute respiratory distress syndrome	164:198	Acute respiratory distress syndrome (ARDS)	164:205	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	9	26	theme	lower	1808:1812	arg1	concentrations					1814:1827	lower concentrations	1808:1827	lower concentrations	1808:1827	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	0	27	theme	respiratory	87:97	arg1	syndrome					108:115	acute respiratory distress syndrome	81:115	acute respiratory distress syndrome in rats	81:123	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	1	28	theme	distress	182:189	arg1	ARDS					201:204	ARDS	201:204	ARDS	201:204	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	1	28	theme	distress	182:189	arg1	syndrome					191:198	Acute respiratory distress syndrome	164:198	Acute respiratory distress syndrome (ARDS)	164:205	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	6	29	theme	injury	998:1003	arg1	markers					1005:1011	cell injury markers	993:1011	cell injury markers	993:1011	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	8	30	theme	NRF2	1530:1533	arg1	pathway					1535:1541	the NRF2 pathway	1526:1541	the NRF2 pathway	1526:1541	Further mechanistic studies demonstrated that HAEGS suppressed oxidative stress by modulating the NRF2 pathway and ameliorated the ARDS through the NF-κB/MAPK signalling pathway.
36662401	7	31	dep	in	1167:1168	arg1	vitro					1170:1174	vitro	1170:1174	vitro	1170:1174	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	11	32	theme	protective	1996:2005	arg1	role					2007:2010	the protective role	1992:2010	the protective role of HAEGS for the first time in managing ARDS	1992:2055	Thus, our work unravels the protective role of HAEGS for the first time in managing ARDS.
36662401	3	33	theme	medicinal	414:422	arg1	plant					424:428	an ancient medicinal plant	403:428	an ancient medicinal plant known to have various therapeutic effects	403:470	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	3	33	theme	medicinal	414:422	arg1	Sylvestre					385:393	Gymnema Sylvestre	377:393	Gymnema Sylvestre (GS)	377:398	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	4	34	dep	in	657:658	arg1	vivo					660:663	vivo	660:663	vivo	660:663	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	7	35	theme	cytokine/chemokine	1338:1355	arg1	expression					1364:1373	cytokine/chemokine marker expression	1338:1373	cytokine/chemokine marker expression	1338:1373	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	3	36	theme	Gymnema	377:383	arg1	plant					424:428	an ancient medicinal plant	403:428	an ancient medicinal plant known to have various therapeutic effects	403:470	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	3	36	theme	Gymnema	377:383	arg1	GS					396:397	GS	396:397	GS	396:397	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	3	36	theme	Gymnema	377:383	arg1	Sylvestre					385:393	Gymnema Sylvestre	377:393	Gymnema Sylvestre (GS)	377:398	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	7	37	theme	LPS-induced	1202:1212	arg1	model					1219:1223	LPS-induced ARDS model	1202:1223	LPS-induced ARDS model	1202:1223	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	0	38	theme	Anti-inflammatory	0:16	arg1	activities					34:43	Anti-inflammatory and antioxidant activities	0:43	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract	0:72	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	7	39	theme	infiltrations	1323:1335	arg1	elevation					1292:1300	the LPS-induced elevation	1276:1300	the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury	1276:1402	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	0	40	theme	antioxidant	22:32	arg1	activities					34:43	Anti-inflammatory and antioxidant activities	0:43	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract	0:72	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	1	41	theme	limited	276:282	arg1	options					296:302	limited therapeutic options	276:302	limited therapeutic options	276:302	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	9	42	theme	fractionation	1622:1634	arg1	results					1636:1642	Additional fractionation results	1611:1642	Additional fractionation results	1611:1642	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	7	43	theme	inflammatory	1305:1316	arg1	infiltrations					1323:1335	inflammatory cell infiltrations	1305:1335	inflammatory cell infiltrations	1305:1335	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	8	44	theme	NF-κB/MAPK	1580:1589	arg1	pathway					1602:1608	the NF-κB/MAPK signalling pathway	1576:1608	the NF-κB/MAPK signalling pathway	1576:1608	Further mechanistic studies demonstrated that HAEGS suppressed oxidative stress by modulating the NRF2 pathway and ameliorated the ARDS through the NF-κB/MAPK signalling pathway.
36662401	5	45	theme	gymnemic	784:791	arg1	acid					793:796	gymnemic acid	784:796	gymnemic acid	784:796	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	6	46	theme	species	1034:1040	arg1	levels					1048:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	5	47	theme	several	747:753	arg1	acid					793:796	gymnemic acid	784:796	gymnemic acid	784:796	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	47	theme	several	747:753	arg1	gymnemasin					829:838	gymnemasin	829:838	gymnemasin	829:838	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	47	theme	several	747:753	arg1	quercetin					841:849	quercetin	841:849	quercetin	841:849	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	47	theme	several	747:753	arg1	gymnemasaponins					799:813	gymnemasaponins	799:813	gymnemasaponins	799:813	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	47	theme	several	747:753	arg1	derivatives					762:772	identified several active derivatives	736:772	identified several active derivatives	736:772	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	47	theme	several	747:753	arg1	gymnemoside					816:826	gymnemoside	816:826	gymnemoside	816:826	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	47	theme	several	747:753	arg1	acids					867:871	long fatty acids	856:871	long fatty acids	856:871	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	2	48	theme	natural	333:339	arg1	sources					341:347	natural sources	333:347	natural sources	333:347	This prompted us to explore natural sources to mitigate this condition.
36662401	0	49	theme	Sylvestre	56:64	arg1	extract					66:72	Gymnema Sylvestre extract	48:72	Gymnema Sylvestre extract	48:72	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	4	50	theme	hydroalcoholic	527:540	arg1	extract					542:548	hydroalcoholic extract	527:548	hydroalcoholic extract of Gymnema Sylvestre (HAEGS)	527:577	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	10	51	theme	signalling	1948:1957	arg1	pathway					1959:1965	the NF-κB/MAPK signalling pathway	1933:1965	the NF-κB/MAPK signalling pathway	1933:1965	Overall, HAEGS significantly mitigated LPS-induced lung injury and ARDS by targeting the NF-κB/MAPK signalling pathway.
36662401	3	52	theme	therapeutic	452:462	arg1	effects					464:470	various therapeutic effects	444:470	various therapeutic effects	444:470	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	1	53	theme	major	221:225	arg1	causes					227:232	the major causes	217:232	the major causes of mortality in COVID-19 patients	217:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	8	54	theme	mechanistic	1440:1450	arg1	studies					1452:1458	Further mechanistic studies	1432:1458	Further mechanistic studies	1432:1458	Further mechanistic studies demonstrated that HAEGS suppressed oxidative stress by modulating the NRF2 pathway and ameliorated the ARDS through the NF-κB/MAPK signalling pathway.
36662401	5	55	theme	active	755:760	arg1	acid					793:796	gymnemic acid	784:796	gymnemic acid	784:796	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	55	theme	active	755:760	arg1	gymnemasin					829:838	gymnemasin	829:838	gymnemasin	829:838	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	55	theme	active	755:760	arg1	quercetin					841:849	quercetin	841:849	quercetin	841:849	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	55	theme	active	755:760	arg1	gymnemasaponins					799:813	gymnemasaponins	799:813	gymnemasaponins	799:813	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	55	theme	active	755:760	arg1	derivatives					762:772	identified several active derivatives	736:772	identified several active derivatives	736:772	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	55	theme	active	755:760	arg1	gymnemoside					816:826	gymnemoside	816:826	gymnemoside	816:826	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	55	theme	active	755:760	arg1	acids					867:871	long fatty acids	856:871	long fatty acids	856:871	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	4	56	theme	HAEGS	572:576	arg1	extract					542:548	hydroalcoholic extract	527:548	hydroalcoholic extract of Gymnema Sylvestre (HAEGS)	527:577	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	7	57	with	pre-treatment	1226:1238	arg1	HAEGS					1245:1249	HAEGS	1245:1249	HAEGS	1245:1249	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	9	58	theme	gymnemic	1708:1715	arg1	derivatives					1722:1732	gymnemic acid derivatives	1708:1732	gymnemic acid derivatives	1708:1732	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	6	59	theme	Gene	874:877	arg1	expression					879:888	Gene expression	874:888	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry	874:936	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	9	60	theme	derivatives	1722:1732	arg1	composition					1693:1703	the exclusive composition	1679:1703	the exclusive composition of gymnemic acid derivatives	1679:1732	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	7	61	with	Consistent	1147:1156	arg1	studies					1176:1182	the in vitro studies	1163:1182	the in vitro studies	1163:1182	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	9	62	dep	effects	1766:1772	arg1	inhibition					1775:1784	inhibition	1775:1784	inhibition of IL-6 and IL-1β	1775:1802	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	5	63	theme	fatty	861:865	arg1	acids					867:871	long fatty acids	856:871	long fatty acids	856:871	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	4	64	theme	therapeutic	505:515	arg1	effect					517:522	the therapeutic effect	501:522	the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models	501:670	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	6	65	theme	cytokine/chemokine	973:990	arg1	levels					1048:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	9	66	theme	better	1741:1746	arg1	effects					1766:1772	better anti-inflammatory effects	1741:1772	better anti-inflammatory effects (inhibition of IL-6 and IL-1β)	1741:1803	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	7	67	theme	dose-dependent	1409:1422	arg1	manner					1424:1429	a dose-dependent manner	1407:1429	a dose-dependent manner	1407:1429	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	1	68	from	patients	259:266	arg1	causes					227:232	the major causes	217:232	the major causes of mortality in COVID-19 patients	217:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	6	69	theme	several	952:958	arg1	levels					1048:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	6	70	theme	HAEGS	1130:1134	arg1	treatment					1136:1144	HAEGS treatment	1130:1144	HAEGS treatment	1130:1144	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	4	71	theme	lung	620:623	arg1	injury					625:630	lipopolysaccharide (LPS)-induced lung injury	587:630	lipopolysaccharide (LPS)-induced lung injury	587:630	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	11	72	theme	HAEGS	2015:2019	arg1	role					2007:2010	the protective role	1992:2010	the protective role of HAEGS for the first time in managing ARDS	1992:2055	Thus, our work unravels the protective role of HAEGS for the first time in managing ARDS.
36662401	7	73	theme	lung	1392:1395	arg1	injury					1397:1402	lung injury	1392:1402	lung injury	1392:1402	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	6	74	theme	flow	923:926	arg1	cytometry					928:936	flow cytometry	923:936	flow cytometry	923:936	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	9	75	theme	IL-6	1789:1792	arg1	inhibition					1775:1784	inhibition	1775:1784	inhibition of IL-6 and IL-1β	1775:1802	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	8	76	theme	oxidative	1495:1503	arg1	stress					1505:1510	oxidative stress	1495:1510	oxidative stress	1495:1510	Further mechanistic studies demonstrated that HAEGS suppressed oxidative stress by modulating the NRF2 pathway and ameliorated the ARDS through the NF-κB/MAPK signalling pathway.
36662401	6	77	theme	oxygen	1027:1032	arg1	ROS					1043:1045	ROS	1043:1045	ROS	1043:1045	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	6	77	theme	oxygen	1027:1032	arg1	species					1034:1040	reactive oxygen species	1018:1040	reactive oxygen species (ROS)	1018:1046	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	9	78	theme	IL-1β	1798:1802	arg1	inhibition					1775:1784	inhibition	1775:1784	inhibition of IL-6 and IL-1β	1775:1802	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	7	79	theme	ROS	1376:1378	arg1	levels					1380:1385	ROS levels	1376:1385	ROS levels	1376:1385	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	5	80	theme	identified	736:745	arg1	acid					793:796	gymnemic acid	784:796	gymnemic acid	784:796	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	80	theme	identified	736:745	arg1	gymnemasin					829:838	gymnemasin	829:838	gymnemasin	829:838	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	80	theme	identified	736:745	arg1	quercetin					841:849	quercetin	841:849	quercetin	841:849	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	80	theme	identified	736:745	arg1	gymnemasaponins					799:813	gymnemasaponins	799:813	gymnemasaponins	799:813	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	80	theme	identified	736:745	arg1	derivatives					762:772	identified several active derivatives	736:772	identified several active derivatives	736:772	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	80	theme	identified	736:745	arg1	gymnemoside					816:826	gymnemoside	816:826	gymnemoside	816:826	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	5	80	theme	identified	736:745	arg1	acids					867:871	long fatty acids	856:871	long fatty acids	856:871	UHPLC-HRMS/GC-MS was employed for characterizing the HAEGS and identified several active derivatives including gymnemic acid, gymnemasaponins, gymnemoside, gymnemasin, quercetin, and long fatty acids.
36662401	10	81	theme	LPS-induced	1887:1897	arg1	injury					1904:1909	LPS-induced lung injury	1887:1909	LPS-induced lung injury	1887:1909	Overall, HAEGS significantly mitigated LPS-induced lung injury and ARDS by targeting the NF-κB/MAPK signalling pathway.
36662401	7	82	theme	expression	1364:1373	arg1	elevation					1292:1300	the LPS-induced elevation	1276:1300	the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury	1276:1402	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	4	83	theme	in	657:658	arg1	models					665:670	in vitro and in vivo models	644:670	in vitro and in vivo models	644:670	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	0	84	theme	distress	99:106	arg1	syndrome					108:115	acute respiratory distress syndrome	81:115	acute respiratory distress syndrome in rats	81:123	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	1	85	theme	respiratory	170:180	arg1	ARDS					201:204	ARDS	201:204	ARDS	201:204	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	1	85	theme	respiratory	170:180	arg1	syndrome					191:198	Acute respiratory distress syndrome	164:198	Acute respiratory distress syndrome (ARDS)	164:205	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	6	86	theme	markers	1005:1011	arg1	levels					1048:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels	952:1053	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	1	87	dep	due	269:271	arg1	one					210:212	one	210:212	one	210:212	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	1	87	dep	due	269:271	arg1	causes					227:232	the major causes	217:232	the major causes of mortality in COVID-19 patients	217:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	6	88	theme	cell	993:996	arg1	markers					1005:1011	cell injury markers	993:1011	cell injury markers	993:1011	Gene expression by RT-qPCR and DCFDA analysis by flow cytometry revealed that several inflammatory cytokine/chemokine, cell injury markers, and reactive oxygen species (ROS) levels were highly upregulated in LPS control and were significantly reduced upon HAEGS treatment.
36662401	7	89	theme	in	1167:1168	arg1	studies					1176:1182	the in vitro studies	1163:1182	the in vitro studies	1163:1182	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	7	90	theme	cell	1318:1321	arg1	infiltrations					1323:1335	inflammatory cell infiltrations	1305:1335	inflammatory cell infiltrations	1305:1335	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	3	91	theme	ancient	406:412	arg1	plant					424:428	an ancient medicinal plant	403:428	an ancient medicinal plant known to have various therapeutic effects	403:470	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	3	91	theme	ancient	406:412	arg1	Sylvestre					385:393	Gymnema Sylvestre	377:393	Gymnema Sylvestre (GS)	377:398	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	9	92	theme	Additional	1611:1620	arg1	results					1636:1642	Additional fractionation results	1611:1642	Additional fractionation results	1611:1642	Additional fractionation results revealed that fraction 6 which has the exclusive composition of gymnemic acid derivatives showed better anti-inflammatory effects (inhibition of IL-6 and IL-1β) at lower concentrations compared to HAEGS.
36662401	8	93	theme	signalling	1591:1600	arg1	pathway					1602:1608	the NF-κB/MAPK signalling pathway	1576:1608	the NF-κB/MAPK signalling pathway	1576:1608	Further mechanistic studies demonstrated that HAEGS suppressed oxidative stress by modulating the NRF2 pathway and ameliorated the ARDS through the NF-κB/MAPK signalling pathway.
36662401	3	94	theme	various	444:450	arg1	effects					464:470	various therapeutic effects	444:470	various therapeutic effects	444:470	Gymnema Sylvestre (GS) is an ancient medicinal plant known to have various therapeutic effects.
36662401	0	95	theme	Gymnema	48:54	arg1	extract					66:72	Gymnema Sylvestre extract	48:72	Gymnema Sylvestre extract	48:72	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	1	96	theme	therapeutic	284:294	arg1	options					296:302	limited therapeutic options	276:302	limited therapeutic options	276:302	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	7	97	theme	LPS-induced	1280:1290	arg1	elevation					1292:1300	the LPS-induced elevation	1276:1300	the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury	1276:1402	Consistent with the in vitro studies, we found that in LPS-induced ARDS model, pre-treatment with HAEGS significantly suppressed the LPS-induced elevation of inflammatory cell infiltrations, cytokine/chemokine marker expression, ROS levels, and lung injury in a dose-dependent manner.
36662401	0	98	theme	extract	66:72	arg1	activities					34:43	Anti-inflammatory and antioxidant activities	0:43	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract	0:72	Anti-inflammatory and antioxidant activities of Gymnema Sylvestre extract rescue acute respiratory distress syndrome in rats via modulating the NF-κB/MAPK pathway.
36662401	4	99	theme	extract	542:548	arg1	effect					517:522	the therapeutic effect	501:522	the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models	501:670	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	10	100	theme	NF-κB/MAPK	1937:1946	arg1	pathway					1959:1965	the NF-κB/MAPK signalling pathway	1933:1965	the NF-κB/MAPK signalling pathway	1933:1965	Overall, HAEGS significantly mitigated LPS-induced lung injury and ARDS by targeting the NF-κB/MAPK signalling pathway.
36662401	1	101	theme	causes	227:232	arg1	one					210:212	one	210:212	one	210:212	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	1	101	theme	causes	227:232	arg1	causes					227:232	the major causes	217:232	the major causes of mortality in COVID-19 patients	217:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
36662401	4	102	dep	in	644:645	arg1	vitro					647:651	vitro	647:651	vitro	647:651	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	4	103	theme	-induced	611:618	arg1	injury					625:630	lipopolysaccharide (LPS)-induced lung injury	587:630	lipopolysaccharide (LPS)-induced lung injury	587:630	This investigation examined the therapeutic effect of hydroalcoholic extract of Gymnema Sylvestre (HAEGS) against lipopolysaccharide (LPS)-induced lung injury and ARDS in in vitro and in vivo models.
36662401	8	104	theme	Further	1432:1438	arg1	studies					1452:1458	Further mechanistic studies	1432:1458	Further mechanistic studies	1432:1458	Further mechanistic studies demonstrated that HAEGS suppressed oxidative stress by modulating the NRF2 pathway and ameliorated the ARDS through the NF-κB/MAPK signalling pathway.
36662401	1	105	theme	mortality	237:245	arg1	causes					227:232	the major causes	217:232	the major causes of mortality in COVID-19 patients	217:266	Acute respiratory distress syndrome (ARDS) is one of the major causes of mortality in COVID-19 patients, due to limited therapeutic options.
35060693	4	0	theme	low-temperature	717:731	arg1	agarose					741:747	low-temperature gelling agarose	717:747	low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays	717:922	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	5	1	theme	real-case	944:952	arg1	feasibility					963:973	the real-case scenario feasibility	940:973	the real-case scenario feasibility of our 3D system	940:990	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	6	2	from	use	1321:1323	arg1	practices					1339:1347	analytical practices	1328:1347	analytical practices	1328:1347	Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.
35060693	4	3	theme	hydrogel	908:915	arg1	assays					917:922	3D hydrogel assays	905:922	3D hydrogel assays	905:922	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	3	4	theme	straightforward	537:551	arg1	fabrication					553:563	their straightforward fabrication	531:563	their straightforward fabrication	531:563	Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties.
35060693	6	5	theme	hydrogel-based	1247:1260	arg1	arrays					1262:1267	hydrogel-based arrays	1247:1267	hydrogel-based arrays	1247:1267	Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.
35060693	4	6	theme	Q3	700:701	arg1	peptide					703:709	Q3 peptide	700:709	Q3 peptide	700:709	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	5	7	theme	system	985:990	arg1	feasibility					963:973	the real-case scenario feasibility	940:973	the real-case scenario feasibility of our 3D system	940:990	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	2	8	theme	analytical	312:321	arg1	surfaces					323:330	analytical surfaces	312:330	analytical surfaces	312:330	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	4	9	theme	composite	624:632	arg1	hydrogel					634:641	a composite hydrogel	622:641	a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays	622:922	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	4	10	theme	gelling	733:739	arg1	agarose					741:747	low-temperature gelling agarose	717:747	low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays	717:922	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	6	11	theme	analytical	1328:1337	arg1	practices					1339:1347	analytical practices	1328:1347	analytical practices	1328:1347	Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.
35060693	4	12	theme	self-assembling	667:681	arg1	peptide					683:689	a self-assembling peptide	665:689	a self-assembling peptide (namely, Q3 peptide)	665:710	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	1	13	theme	Canonical	85:93	arg1	immunoassays					95:106	Canonical immunoassays	85:106	Canonical immunoassays	85:106	Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits.
35060693	2	14	from	immobilization	241:254	arg1	spots					301:305	spatially discrete three-dimensional (3D) spots	259:305	spatially discrete three-dimensional (3D) spots onto analytical surfaces	259:330	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	1	15	theme	biomolecular	197:208	arg1	baits					210:214	biomolecular baits	197:214	biomolecular baits	197:214	Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits.
35060693	5	16	theme	scenario	954:961	arg1	feasibility					963:973	the real-case scenario feasibility	940:973	the real-case scenario feasibility of our 3D system	940:990	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	6	17	theme	two-component	1197:1209	arg1	hydrogel					1211:1218	the new two-component hydrogel	1189:1218	the new two-component hydrogel	1189:1218	Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.
35060693	3	18	theme	functional	578:587	arg1	properties					589:598	optimal functional properties	570:598	optimal functional properties	570:598	Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties.
35060693	2	19	dep	three-dimensional	278:294	arg1	3D					297:298	3D	297:298	3D	297:298	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	4	20	theme	3D	905:906	arg1	assays					917:922	3D hydrogel assays	905:922	3D hydrogel assays	905:922	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	4	21	theme	simple	772:777	arg1	application					790:800	simple and robust application	772:800	simple and robust application	772:800	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	3	22	theme	optimal	570:576	arg1	properties					589:598	optimal functional properties	570:598	optimal functional properties	570:598	Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties.
35060693	3	23	theme	broad	458:462	arg1	application					464:474	the broad application	454:474	the broad application of 3D systems	454:488	Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties.
35060693	0	24	theme	Composite	0:8	arg1	Peptide-Agarose					10:24	Composite Peptide-Agarose	0:24	Composite Peptide-Agarose	0:24	Composite Peptide-Agarose Hydrogels for Robust and High-Sensitivity 3D Immunoassays.
35060693	6	25	theme	routine	1313:1319	arg1	use					1321:1323	their routine use	1307:1323	their routine use in analytical practices	1307:1347	Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.
35060693	5	26	theme	signal-to-noise	1092:1106	arg1	ratio					1108:1112	remarkably improved signal-to-noise ratio	1072:1112	remarkably improved signal-to-noise ratio	1072:1112	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	5	27	theme	3D	982:983	arg1	system					985:990	our 3D system	978:990	our 3D system	978:990	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	2	28	theme	hydrogel	335:342	arg1	encapsulation					344:356	hydrogel encapsulation	335:356	hydrogel encapsulation	335:356	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	4	29	contain	have	767:770	arg1	agarose					741:747	low-temperature gelling agarose	717:747	low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays	717:922	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	4	29	contain	have	767:770	arg2	application					790:800	simple and robust application	772:800	simple and robust application	772:800	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	2	30	dep	two-dimensional	417:431	arg1	2D					434:435	2D	434:435	2D	434:435	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	1	31	theme	sensitive	123:131	arg1	capturing					146:154	highly sensitive and specific capturing	116:154	highly sensitive and specific capturing of circulating biomarkers	116:180	Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits.
35060693	1	32	theme	circulating	159:169	arg1	biomarkers					171:180	circulating biomarkers	159:180	circulating biomarkers	159:180	Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits.
35060693	2	33	theme	discrete	269:276	arg1	spots					301:305	spatially discrete three-dimensional (3D) spots	259:305	spatially discrete three-dimensional (3D) spots onto analytical surfaces	259:330	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	6	34	theme	arrays	1262:1267	arg1	perspectives					1231:1242	the perspectives	1227:1242	the perspectives of hydrogel-based arrays	1227:1267	Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.
35060693	3	35	theme	systems	482:488	arg1	application					464:474	the broad application	454:474	the broad application of 3D systems	454:488	Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties.
35060693	0	36	theme	Robust	40:45	arg1	Immunoassays					71:82	Robust and High-Sensitivity 3D Immunoassays	40:82	Robust and High-Sensitivity 3D Immunoassays	40:82	Composite Peptide-Agarose Hydrogels for Robust and High-Sensitivity 3D Immunoassays.
35060693	2	37	theme	spatially	259:267	arg1	spots					301:305	spatially discrete three-dimensional (3D) spots	259:305	spatially discrete three-dimensional (3D) spots onto analytical surfaces	259:330	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	0	38	theme	3D	68:69	arg1	Immunoassays					71:82	Robust and High-Sensitivity 3D Immunoassays	40:82	Robust and High-Sensitivity 3D Immunoassays	40:82	Composite Peptide-Agarose Hydrogels for Robust and High-Sensitivity 3D Immunoassays.
35060693	5	39	theme	2D	1143:1144	arg1	format					1146:1151	the 2D format	1139:1151	the 2D format	1139:1151	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	4	40	dep	peptide	683:689	arg1	peptide					703:709	Q3 peptide	700:709	Q3 peptide	700:709	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	5	41	theme	exquisite	1157:1165	arg1	specificity					1167:1177	exquisite specificity	1157:1177	exquisite specificity	1157:1177	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	2	42	theme	relevant	379:386	arg1	advantages					388:397	relevant advantages	379:397	relevant advantages	379:397	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	0	43	theme	High-Sensitivity	51:66	arg1	Immunoassays					71:82	Robust and High-Sensitivity 3D Immunoassays	40:82	Robust and High-Sensitivity 3D Immunoassays	40:82	Composite Peptide-Agarose Hydrogels for Robust and High-Sensitivity 3D Immunoassays.
35060693	2	44	theme	bioprobe	232:239	arg1	immobilization					241:254	bioprobe immobilization	232:254	bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation	232:356	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	5	45	theme	improved	1083:1090	arg1	ratio					1108:1112	remarkably improved signal-to-noise ratio	1072:1112	remarkably improved signal-to-noise ratio	1072:1112	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	1	46	theme	specific	137:144	arg1	capturing					146:154	highly sensitive and specific capturing	116:154	highly sensitive and specific capturing of circulating biomarkers	116:180	Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits.
35060693	4	47	theme	arrays	837:842	arg1	fabrication					809:819	the fabrication	805:819	the fabrication of microdroplet arrays	805:842	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	5	48	theme	serum	1031:1035	arg1	immunoreactivity					1041:1056	Covid-19 patients' serum IgG immunoreactivity	1012:1056	Covid-19 patients' serum IgG immunoreactivity	1012:1056	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	4	49	theme	microdroplet	824:835	arg1	arrays					837:842	microdroplet arrays	824:842	microdroplet arrays	824:842	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	4	50	theme	robust	783:788	arg1	application					790:800	simple and robust application	772:800	simple and robust application	772:800	Herein, we report on a composite hydrogel obtained by combining a self-assembling peptide (namely, Q3 peptide) with low-temperature gelling agarose that is proved to have simple and robust application in the fabrication of microdroplet arrays, overcoming hurdles and limitations commonly associated with 3D hydrogel assays.
35060693	5	51	theme	IgG	1037:1039	arg1	immunoreactivity					1041:1056	Covid-19 patients' serum IgG immunoreactivity	1012:1056	Covid-19 patients' serum IgG immunoreactivity	1012:1056	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	1	52	theme	biomarkers	171:180	arg1	capturing					146:154	highly sensitive and specific capturing	116:154	highly sensitive and specific capturing of circulating biomarkers	116:180	Canonical immunoassays rely on highly sensitive and specific capturing of circulating biomarkers by interacting biomolecular baits.
35060693	5	53	theme	canonical	1119:1127	arg1	assays					1129:1134	canonical assays	1119:1134	canonical assays	1119:1134	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	5	54	theme	immunoreactivity	1041:1056	arg1	profiling					999:1007	the profiling	995:1007	the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity	995:1177	We demonstrate the real-case scenario feasibility of our 3D system in the profiling of Covid-19 patients' serum IgG immunoreactivity, which showed remarkably improved signal-to-noise ratio over canonical assays in the 2D format and exquisite specificity.
35060693	2	55	theme	two-dimensional	417:431	arg1	platforms					438:446	conventional two-dimensional (2D) platforms	404:446	conventional two-dimensional (2D) platforms	404:446	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	3	56	theme	3D	479:480	arg1	systems					482:488	3D systems	479:488	3D systems	479:488	Yet, the broad application of 3D systems is still hampered by hurdles in matching their straightforward fabrication with optimal functional properties.
35060693	6	57	theme	new	1193:1195	arg1	hydrogel					1211:1218	the new two-component hydrogel	1189:1218	the new two-component hydrogel	1189:1218	Overall, the new two-component hydrogel widens the perspectives of hydrogel-based arrays and represents a step forward towards their routine use in analytical practices.
35060693	2	58	theme	three-dimensional	278:294	arg1	spots					301:305	spatially discrete three-dimensional (3D) spots	259:305	spatially discrete three-dimensional (3D) spots onto analytical surfaces	259:330	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
35060693	2	59	theme	conventional	404:415	arg1	platforms					438:446	conventional two-dimensional (2D) platforms	404:446	conventional two-dimensional (2D) platforms	404:446	In this frame, bioprobe immobilization in spatially discrete three-dimensional (3D) spots onto analytical surfaces by hydrogel encapsulation was shown to provide relevant advantages over conventional two-dimensional (2D) platforms.
36259730	2	0	theme	optimal	395:401	arg1	unknown					440:446	unknown	440:446	unknown	440:446	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	2	0	theme	optimal	395:401	arg1	ratio					431:435	the optimal protamine-to-heparin dosing ratio	391:435	the optimal protamine-to-heparin dosing ratio	391:435	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	12	1	theme	infarction	1889:1898	arg1	low					1904:1906	low	1904:1906	low	1904:1906	The occurrence of stroke and myocardial infarction was low and comparable between both groups.
36259730	12	1	theme	infarction	1889:1898	arg1	occurrence					1853:1862	The occurrence	1849:1862	The occurrence of stroke and myocardial infarction	1849:1898	The occurrence of stroke and myocardial infarction was low and comparable between both groups.
36259730	3	2	theme	bleeding	570:577	arg1	complications					579:591	bleeding complications	570:591	bleeding complications after TAVR	570:602	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	7	3	theme	heparin	927:933	arg1	antagonization					909:922	Full antagonization	904:922	Full antagonization of heparin	904:933	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	2	4	theme	lower	316:320	arg1	rates					331:335	lower bleeding rates	316:335	lower bleeding rates in patients receiving protamine for heparin reversal	316:388	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	8	5	theme	major	1206:1210	arg1	complications					1221:1233	major vascular complications	1206:1233	major vascular complications	1206:1233	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	8	6	theme	complications	1221:1233	arg1	lower					1253:1257	lower	1253:1257	lower	1253:1257	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	8	6	theme	complications	1221:1233	arg1	incidence					1193:1201	the incidence	1189:1201	the incidence of major vascular complications	1189:1233	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	7	7	theme	life-threatening	1092:1107	arg1	rates					1083:1087	lower rates	1077:1087	lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01)	1077:1176	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	4	8	theme	full	700:703	arg1	antagonization					713:726	partial and 823 full heparin antagonization	684:726	partial and 823 full heparin antagonization	684:726	METHODS The study included 1446 patients undergoing TAVR, of whom 623 received partial and 823 full heparin antagonization.
36259730	10	9	theme	myocardial	1535:1544	arg1	infarction					1546:1555	myocardial infarction	1535:1555	myocardial infarction	1535:1555	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	11	10	theme	heparin	1697:1703	arg1	antagonization					1705:1718	CONCLUSIONS Full heparin antagonization	1680:1718	CONCLUSIONS Full heparin antagonization	1680:1718	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	5	11	theme	mortality	776:784	arg1	endpoint					741:748	The primary endpoint	729:748	The primary endpoint	729:748	The primary endpoint was a composite of 30-day mortality, life-threatening, and major bleeding.
36259730	5	11	theme	mortality	776:784	arg1	composite					756:764	a composite	754:764	a composite of 30-day mortality	754:784	The primary endpoint was a composite of 30-day mortality, life-threatening, and major bleeding.
36259730	5	11	theme	mortality	776:784	arg1	mortality					776:784	30-day mortality	769:784	30-day mortality	769:784	The primary endpoint was a composite of 30-day mortality, life-threatening, and major bleeding.
36259730	9	12	theme	partial	1416:1422	arg1	antagonization					1432:1445	partial heparin antagonization	1416:1445	partial heparin antagonization	1416:1445	The need for red-blood-cell transfusion was lower in patients receiving full as compared to partial heparin antagonization (10.4% vs. 15.9%, p < .01).
36259730	10	13	theme	stroke	1524:1529	arg1	incidence					1511:1519	the incidence	1507:1519	the incidence of stroke and myocardial infarction	1507:1555	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	11	14	theme	CONCLUSIONS	1680:1690	arg1	antagonization					1705:1718	CONCLUSIONS Full heparin antagonization	1680:1718	CONCLUSIONS Full heparin antagonization	1680:1718	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	9	15	dep	lower	1368:1372	arg1	%					1452:1452	10.4%	1448:1452	10.4%	1448:1452	The need for red-blood-cell transfusion was lower in patients receiving full as compared to partial heparin antagonization (10.4% vs. 15.9%, p < .01).
36259730	9	15	dep	lower	1368:1372	arg1	%					1462:1462	15.9%	1458:1462	15.9%	1458:1462	The need for red-blood-cell transfusion was lower in patients receiving full as compared to partial heparin antagonization (10.4% vs. 15.9%, p < .01).
36259730	10	16	theme	p = .73	1629:1635	arg1	%					1644:1644	p = .73 and 0.2%	1629:1644	%	1644:1644	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	1	17	theme	major	160:164	arg1	advances					166:173	major advances	160:173	major advances	160:173	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	8	18	theme	heparin	1281:1287	arg1	reversal					1289:1296	full heparin reversal	1276:1296	full heparin reversal	1276:1296	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	7	19	dep	%	1027:1027	arg1	p < .01					1040:1046	p < .01	1040:1046	p < .01	1040:1046	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	0	20	theme	bleeding	64:71	arg1	complications					73:85	bleeding complications	64:85	bleeding complications in patients undergoing TAVR-A multicenter	64:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	4	21	dep	METHODS	605:611	arg1	included					623:630	included	623:630	included 1446 patients undergoing TAVR, of whom 623 received partial and 823 full heparin antagonization	623:726	METHODS The study included 1446 patients undergoing TAVR, of whom 623 received partial and 823 full heparin antagonization.
36259730	1	22	theme	aortic	190:195	arg1	TAVR					216:219	TAVR	216:219	TAVR	216:219	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	1	22	theme	aortic	190:195	arg1	replacement					203:213	transcatheter aortic valve replacement	176:213	transcatheter aortic valve replacement (TAVR)	176:220	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	7	23	dep	%	1157:1157	arg1	p < .01					1169:1175	p < .01	1169:1175	p < .01	1169:1175	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	0	24	from	prevention	50:59	arg1	patients					90:97	patients	90:97	patients undergoing TAVR-A multicenter	90:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	3	25	theme	heparin	516:522	arg1	regimens					539:546	two different heparin antagonization regimens	502:546	two different heparin antagonization regimens for the prevention of bleeding complications after TAVR	502:602	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	10	26	theme	full	1579:1582	arg1	reversal					1604:1611	full and partial heparin reversal	1579:1611	full and partial heparin reversal	1579:1611	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	7	27	theme	partial	998:1004	arg1	reversal					1014:1021	partial heparin reversal	998:1021	partial heparin reversal	998:1021	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	3	28	theme	study	476:480	arg1	aim					464:466	The aim	460:466	The aim of this study	460:480	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	3	28	theme	study	476:480	arg1	comparison					488:497	a comparison	486:497	a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR	486:602	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	0	29	theme	Optimal	0:6	arg1	ratio					36:40	Optimal protamine-to-heparin dosing ratio	0:40	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter	0:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	9	30	dep	%	1452:1452	arg1	p < .01					1465:1471	p < .01	1465:1471	p < .01	1465:1471	The need for red-blood-cell transfusion was lower in patients receiving full as compared to partial heparin antagonization (10.4% vs. 15.9%, p < .01).
36259730	3	31	theme	different	506:514	arg1	regimens					539:546	two different heparin antagonization regimens	502:546	two different heparin antagonization regimens for the prevention of bleeding complications after TAVR	502:602	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	0	32	theme	dosing	29:34	arg1	ratio					36:40	Optimal protamine-to-heparin dosing ratio	0:40	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter	0:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	10	33	theme	0.2	1641:1643	arg1	%					1644:1644	p = .73 and 0.2%	1629:1644	%	1644:1644	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	8	34	with	patients	1262:1269	arg1	reversal					1289:1296	full heparin reversal	1276:1296	full heparin reversal	1276:1296	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	10	35	theme	partial	1588:1594	arg1	reversal					1604:1611	full and partial heparin reversal	1579:1611	full and partial heparin reversal	1579:1611	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	7	36	theme	bleeding	1144:1151	arg1	rates					1083:1087	lower rates	1077:1087	lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01)	1077:1176	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	3	37	dep	comparison	488:497	arg1	HYPOTHESIS					449:458	HYPOTHESIS	449:458	HYPOTHESIS	449:458	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	3	38	theme	antagonization	524:537	arg1	regimens					539:546	two different heparin antagonization regimens	502:546	two different heparin antagonization regimens for the prevention of bleeding complications after TAVR	502:602	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	11	39	theme	life-threatening	1761:1776	arg1	rates					1752:1756	significantly lower rates	1732:1756	significantly lower rates of life-threatening and major bleeding after TAVR	1732:1806	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	7	40	theme	primary	966:972	arg1	endpoint					974:981	the primary endpoint	962:981	the primary endpoint	962:981	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	10	41	with	patients	1565:1572	arg1	reversal					1604:1611	full and partial heparin reversal	1579:1611	full and partial heparin reversal	1579:1611	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	8	42	dep	lower	1253:1257	arg1	%					1302:1302	3.5%	1299:1302	3.5%	1299:1302	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	8	42	dep	lower	1253:1257	arg1	%					1311:1311	7.5%	1308:1311	7.5%	1308:1311	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	2	43	theme	protamine-to-heparin	403:422	arg1	unknown					440:446	unknown	440:446	unknown	440:446	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	2	43	theme	protamine-to-heparin	403:422	arg1	ratio					431:435	the optimal protamine-to-heparin dosing ratio	391:435	the optimal protamine-to-heparin dosing ratio	391:435	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	9	44	theme	red-blood-cell	1337:1350	arg1	transfusion					1352:1362	red-blood-cell transfusion	1337:1362	red-blood-cell transfusion	1337:1362	The need for red-blood-cell transfusion was lower in patients receiving full as compared to partial heparin antagonization (10.4% vs. 15.9%, p < .01).
36259730	7	45	theme	lower	947:951	arg1	rates					953:957	lower rates	947:957	lower rates of the primary endpoint	947:981	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	12	46	theme	myocardial	1878:1887	arg1	infarction					1889:1898	myocardial infarction	1878:1898	myocardial infarction	1878:1898	The occurrence of stroke and myocardial infarction was low and comparable between both groups.
36259730	6	47	theme	myocardial	862:871	arg1	infarction					873:882	myocardial infarction	862:882	myocardial infarction	862:882	Safety endpoints included stroke and myocardial infarction at 30 days.
36259730	2	48	theme	bleeding	322:329	arg1	rates					331:335	lower bleeding rates	316:335	lower bleeding rates in patients receiving protamine for heparin reversal	316:388	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	1	49	theme	valve	197:201	arg1	TAVR					216:219	TAVR	216:219	TAVR	216:219	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	1	49	theme	valve	197:201	arg1	replacement					203:213	transcatheter aortic valve replacement	176:213	transcatheter aortic valve replacement (TAVR)	176:220	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	4	50	theme	heparin	705:711	arg1	antagonization					713:726	partial and 823 full heparin antagonization	684:726	partial and 823 full heparin antagonization	684:726	METHODS The study included 1446 patients undergoing TAVR, of whom 623 received partial and 823 full heparin antagonization.
36259730	7	51	theme	Full	904:907	arg1	antagonization					909:922	Full antagonization	904:922	Full antagonization of heparin	904:933	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	12	52	theme	stroke	1867:1872	arg1	low					1904:1906	low	1904:1906	low	1904:1906	The occurrence of stroke and myocardial infarction was low and comparable between both groups.
36259730	12	52	theme	stroke	1867:1872	arg1	occurrence					1853:1862	The occurrence	1849:1862	The occurrence of stroke and myocardial infarction	1849:1898	The occurrence of stroke and myocardial infarction was low and comparable between both groups.
36259730	0	53	from	patients	90:97	arg1	prevention					50:59	the prevention	46:59	the prevention of bleeding complications in patients undergoing TAVR-A multicenter	46:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	4	54	theme	partial	684:690	arg1	antagonization					713:726	partial and 823 full heparin antagonization	684:726	partial and 823 full heparin antagonization	684:726	METHODS The study included 1446 patients undergoing TAVR, of whom 623 received partial and 823 full heparin antagonization.
36259730	10	55	theme	infarction	1546:1555	arg1	incidence					1511:1519	the incidence	1507:1519	the incidence of stroke and myocardial infarction	1507:1555	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	10	56	dep	observed	1495:1502	arg1	%					1617:1617	2.2%	1614:1617	2.2%	1614:1617	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	10	56	dep	observed	1495:1502	arg1	%					1626:1626	2.6%	1623:1626	2.6%	1623:1626	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	8	57	dep	%	1302:1302	arg1	p < .01					1314:1320	p < .01	1314:1320	p < .01	1314:1320	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	0	58	from	complications	73:85	arg1	patients					90:97	patients	90:97	patients undergoing TAVR-A multicenter	90:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	7	59	dep	%	1113:1113	arg1	p = .05					1125:1131	p = .05	1125:1131	p = .05	1125:1131	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	5	60	theme	primary	733:739	arg1	endpoint					741:748	The primary endpoint	729:748	The primary endpoint	729:748	The primary endpoint was a composite of 30-day mortality, life-threatening, and major bleeding.
36259730	5	60	theme	primary	733:739	arg1	composite					756:764	a composite	754:764	a composite of 30-day mortality	754:784	The primary endpoint was a composite of 30-day mortality, life-threatening, and major bleeding.
36259730	5	60	theme	primary	733:739	arg1	mortality					776:784	30-day mortality	769:784	30-day mortality	769:784	The primary endpoint was a composite of 30-day mortality, life-threatening, and major bleeding.
36259730	11	61	theme	heparin	1831:1837	arg1	reversal					1839:1846	partial heparin reversal	1823:1846	partial heparin reversal	1823:1846	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	9	62	theme	heparin	1424:1430	arg1	antagonization					1432:1445	partial heparin antagonization	1416:1445	partial heparin antagonization	1416:1445	The need for red-blood-cell transfusion was lower in patients receiving full as compared to partial heparin antagonization (10.4% vs. 15.9%, p < .01).
36259730	11	63	theme	Full	1692:1695	arg1	antagonization					1705:1718	CONCLUSIONS Full heparin antagonization	1680:1718	CONCLUSIONS Full heparin antagonization	1680:1718	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	0	64	theme	complications	73:85	arg1	prevention					50:59	the prevention	46:59	the prevention of bleeding complications in patients undergoing TAVR-A multicenter	46:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	2	65	theme	previous	290:297	arg1	studies					299:305	previous studies	290:305	previous studies	290:305	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	2	66	from	rates	331:335	arg1	patients					340:347	patients	340:347	patients receiving protamine	340:367	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	11	67	theme	partial	1823:1829	arg1	reversal					1839:1846	partial heparin reversal	1823:1846	partial heparin reversal	1823:1846	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	8	68	theme	full	1276:1279	arg1	reversal					1289:1296	full heparin reversal	1276:1296	full heparin reversal	1276:1296	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	7	69	dep	compared	986:993	arg1	%					1037:1037	10.4%	1033:1037	10.4%	1033:1037	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	7	69	dep	compared	986:993	arg1	%					1027:1027	5.6%	1024:1027	5.6%	1024:1027	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	1	70	theme	transcatheter	176:188	arg1	TAVR					216:219	TAVR	216:219	TAVR	216:219	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	1	70	theme	transcatheter	176:188	arg1	replacement					203:213	transcatheter aortic valve replacement	176:213	transcatheter aortic valve replacement (TAVR)	176:220	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	0	71	theme	TAVR-A	110:115	arg1	multicenter					117:127	TAVR-A multicenter	110:127	TAVR-A multicenter	110:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	10	72	located	observed	1495:1502	arg1	incidence					1511:1519	the incidence	1507:1519	the incidence of stroke and myocardial infarction	1507:1555	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	10	72	located	observed	1495:1502	arg2	differences					1478:1488	No differences	1475:1488	No differences	1475:1488	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	3	73	theme	regimens	539:546	arg1	aim					464:466	The aim	460:466	The aim of this study	460:480	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	3	73	theme	regimens	539:546	arg1	comparison					488:497	a comparison	486:497	a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR	486:602	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36259730	8	74	theme	vascular	1212:1219	arg1	complications					1221:1233	major vascular complications	1206:1233	major vascular complications	1206:1233	Moreover, the incidence of major vascular complications was significantly lower in patients with full heparin reversal (3.5% vs. 7.5%, p < .01).
36259730	1	75	dep	BACKGROUND	141:150	arg1	associated					231:240	associated	231:240	is still associated with procedure-specific complications	222:278	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	0	76	theme	protamine-to-heparin	8:27	arg1	ratio					36:40	Optimal protamine-to-heparin dosing ratio	0:40	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter	0:127	Optimal protamine-to-heparin dosing ratio for the prevention of bleeding complications in patients undergoing TAVR-A multicenter experience.
36259730	7	77	theme	heparin	1006:1012	arg1	reversal					1014:1021	partial heparin reversal	998:1021	partial heparin reversal	998:1021	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	7	78	theme	lower	1077:1081	arg1	rates					1083:1087	lower rates	1077:1087	lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01)	1077:1176	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	5	79	theme	30-day	769:774	arg1	mortality					776:784	30-day mortality	769:784	30-day mortality	769:784	The primary endpoint was a composite of 30-day mortality, life-threatening, and major bleeding.
36259730	11	80	theme	bleeding	1788:1795	arg1	rates					1752:1756	significantly lower rates	1732:1756	significantly lower rates of life-threatening and major bleeding after TAVR	1732:1806	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	10	81	dep	%	1617:1617	arg1	%					1644:1644	p = .73 and 0.2%	1629:1644	%	1644:1644	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	10	81	dep	%	1617:1617	arg1	%					1653:1653	0.4%	1650:1653	0.4%	1650:1653	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	10	81	dep	%	1617:1617	arg1	p = .64					1656:1662	p = .64	1656:1662	p = .64	1656:1662	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	10	82	theme	heparin	1596:1602	arg1	reversal					1604:1611	full and partial heparin reversal	1579:1611	full and partial heparin reversal	1579:1611	No differences were observed in the incidence of stroke and myocardial infarction between patients with full and partial heparin reversal (2.2% vs. 2.6%, p = .73 and 0.2% vs. 0.4%, p = .64, respectively).
36259730	9	83	dep	full	1396:1399	arg1	compared					1404:1411	compared	1404:1411	compared to partial heparin antagonization	1404:1445	The need for red-blood-cell transfusion was lower in patients receiving full as compared to partial heparin antagonization (10.4% vs. 15.9%, p < .01).
36259730	6	84	theme	Safety	825:830	arg1	endpoints					832:840	Safety endpoints	825:840	Safety endpoints	825:840	Safety endpoints included stroke and myocardial infarction at 30 days.
36259730	2	85	theme	dosing	424:429	arg1	unknown					440:446	unknown	440:446	unknown	440:446	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	2	85	theme	dosing	424:429	arg1	ratio					431:435	the optimal protamine-to-heparin dosing ratio	391:435	the optimal protamine-to-heparin dosing ratio	391:435	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	11	86	theme	lower	1746:1750	arg1	rates					1752:1756	significantly lower rates	1732:1756	significantly lower rates of life-threatening and major bleeding after TAVR	1732:1806	CONCLUSIONS Full heparin antagonization resulted in significantly lower rates of life-threatening and major bleeding after TAVR as compared to partial heparin reversal.
36259730	7	87	theme	endpoint	974:981	arg1	rates					953:957	lower rates	947:957	lower rates of the primary endpoint	947:981	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	2	88	theme	heparin	373:379	arg1	reversal					381:388	heparin reversal	373:388	heparin reversal	373:388	Although previous studies reported lower bleeding rates in patients receiving protamine for heparin reversal, the optimal protamine-to-heparin dosing ratio is unknown.
36259730	1	89	theme	procedure-specific	247:264	arg1	complications					266:278	procedure-specific complications	247:278	procedure-specific complications	247:278	BACKGROUND Despite major advances, transcatheter aortic valve replacement (TAVR) is still associated with procedure-specific complications.
36259730	7	90	dep	RESULTS	896:902	arg1	resulted					935:942	resulted	935:942	resulted	935:942	RESULTS Full antagonization of heparin resulted in lower rates of the primary endpoint as compared to partial heparin reversal (5.6% vs. 10.4%, p < .01), which was mainly driven by lower rates of life-threatening (0.5% vs. 1.6%, p = .05) and major bleeding (3.2% vs. 7.5%, p < .01).
36259730	3	91	theme	complications	579:591	arg1	prevention					556:565	the prevention	552:565	the prevention of bleeding complications after TAVR	552:602	HYPOTHESIS The aim of this study was a comparison of two different heparin antagonization regimens for the prevention of bleeding complications after TAVR.
36760122	8	0	theme	knowledge	1079:1087	arg1	gap					1089:1091	the knowledge gap	1075:1091	the knowledge gap	1075:1091	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	4	1	theme	unique	506:511	arg1	compositions					519:530	the unique sugar compositions	502:530	the unique sugar compositions found in such functional glycans on DG	502:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	2	theme	protein	689:695	arg1	actions					643:649	the sequential actions	628:649	the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate	628:746	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	1	3	theme	skeletal	252:259	arg1	membranes					273:281	skeletal muscle cell membranes	252:281	skeletal muscle cell membranes	252:281	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	8	4	from	present	1122:1128	arg1	mammals					1133:1139	mammals	1133:1139	mammals	1133:1139	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	6	5	used	used	931:934	arg2	they					926:929	they	926:929	they	926:929	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	4	6	theme	fukutin-related	673:687	arg1	FKRP					698:701	FKRP	698:701	FKRP	698:701	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	6	theme	fukutin-related	673:687	arg1	protein					689:695	fukutin-related protein	673:695	fukutin-related protein (FKRP)	673:702	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	1	7	theme	muscle	261:266	arg1	membranes					273:281	skeletal muscle cell membranes	252:281	skeletal muscle cell membranes	252:281	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	4	8	theme	compositions	519:530	arg1	compositions					519:530	the unique sugar compositions	502:530	the unique sugar compositions found in such functional glycans on DG	502:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	8	theme	compositions	519:530	arg1	One					495:497	One	495:497	One	495:497	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	9	theme	donor	732:736	arg1	CDP-ribitol					715:725	CDP-ribitol	715:725	CDP-ribitol	715:725	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	9	theme	donor	732:736	arg1	substrate					738:746	a donor substrate	730:746	a donor substrate	730:746	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	1	10	theme	cell	268:271	arg1	membranes					273:281	skeletal muscle cell membranes	252:281	skeletal muscle cell membranes	252:281	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	3	11	theme	functional	368:377	arg1	glycosylation					379:391	Fully functional glycosylation	362:391	Fully functional glycosylation	362:391	Fully functional glycosylation is required for this domain to interact with components of extracellular matrices, including laminin.
36760122	2	12	theme	extracellular	288:300	arg1	DG					312:313	DG	312:313	DG	312:313	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	2	12	theme	extracellular	288:300	arg1	domain					302:307	The extracellular domain	284:307	The extracellular domain of DG	284:313	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	2	12	theme	extracellular	288:300	arg1	α-DG					316:319	α-DG	316:319	α-DG	316:319	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	1	13	theme	transmembrane	101:113	arg1	protein					115:121	a muscular transmembrane protein	90:121	a muscular transmembrane protein	90:121	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	1	13	theme	transmembrane	101:113	arg1	Dystroglycan					71:82	Dystroglycan	71:82	Dystroglycan (DG)	71:87	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	1	14	link	matrix-derived	175:188	arg1	signals					190:196	extracellular matrix-derived signals	161:196	extracellular matrix-derived signals	161:196	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	4	15	theme	fukutin	654:660	arg1	actions					643:649	the sequential actions	628:649	the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate	628:746	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	8	16	dep	Imae	1053:1056	arg1	al.					1061:1063	Imae et al.	1053:1063	Imae et al.	1053:1063	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	1	17	theme	extracellular	161:173	arg1	signals					190:196	extracellular matrix-derived signals	161:196	extracellular matrix-derived signals	161:196	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	6	18	theme	glycerol	847:854	arg1	GroP					867:870	GroP	867:870	GroP	867:870	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	6	18	theme	glycerol	847:854	arg1	phosphate					856:864	glycerol phosphate	847:864	glycerol phosphate (GroP)	847:871	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	1	19	theme	matrix-derived	175:188	arg1	signals					190:196	extracellular matrix-derived signals	161:196	extracellular matrix-derived signals	161:196	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	0	20	theme	Inhibitory	0:9	arg1	machinery					11:19	Inhibitory machinery	0:19	Inhibitory machinery for the functional glycosylation of dystroglycan	0:68	Inhibitory machinery for the functional glycosylation of dystroglycan.
36760122	7	21	theme	findings	1027:1034	arg1	relevance					1008:1016	the physiological relevance	990:1016	the physiological relevance of these findings	990:1034	However, the physiological relevance of these findings remains elusive.
36760122	5	22	theme	matriglycan	783:793	arg1	biosynthesis					795:806	matriglycan biosynthesis	783:806	matriglycan biosynthesis	783:806	These are then further primed for matriglycan biosynthesis.
36760122	7	23	theme	physiological	994:1006	arg1	relevance					1008:1016	the physiological relevance	990:1016	the physiological relevance of these findings	990:1034	However, the physiological relevance of these findings remains elusive.
36760122	2	24	theme	DG	312:313	arg1	DG					312:313	DG	312:313	DG	312:313	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	2	24	theme	DG	312:313	arg1	domain					302:307	The extracellular domain	284:307	The extracellular domain of DG	284:313	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	2	24	theme	DG	312:313	arg1	α-DG					316:319	α-DG	316:319	α-DG	316:319	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	6	25	dep	in	818:819	arg1	vitro					821:825	vitro	821:825	vitro	821:825	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	1	26	theme	muscular	92:99	arg1	protein					115:121	a muscular transmembrane protein	90:121	a muscular transmembrane protein	90:121	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	1	26	theme	muscular	92:99	arg1	Dystroglycan					71:82	Dystroglycan	71:82	Dystroglycan (DG)	71:87	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	4	27	from	glycans	557:563	arg1	DG					568:569	DG	568:569	DG	568:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	3	28	theme	matrices	466:473	arg1	components					438:447	components	438:447	components	438:447	Fully functional glycosylation is required for this domain to interact with components of extracellular matrices, including laminin.
36760122	3	28	theme	matrices	466:473	arg1	laminin					486:492	laminin	486:492	laminin	486:492	Fully functional glycosylation is required for this domain to interact with components of extracellular matrices, including laminin.
36760122	0	29	theme	functional	29:38	arg1	glycosylation					40:52	the functional glycosylation	25:52	the functional glycosylation of dystroglycan	25:68	Inhibitory machinery for the functional glycosylation of dystroglycan.
36760122	4	30	theme	such	541:544	arg1	glycans					557:563	such functional glycans	541:563	such functional glycans on DG	541:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	31	theme	ribitol	578:584	arg1	RboP					598:601	RboP	598:601	RboP	598:601	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	31	theme	ribitol	578:584	arg1	phosphates					586:595	two ribitol phosphates	574:595	two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate	574:746	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	8	32	attach	present	1122:1128	arg2	CDP-Gro					1111:1117	CDP-Gro	1111:1117	CDP-Gro	1111:1117	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	8	32	attach	present	1122:1128	arg1	mammals					1133:1139	mammals	1133:1139	mammals	1133:1139	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	4	33	theme	sequential	632:641	arg1	actions					643:649	the sequential actions	628:649	the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate	628:746	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	0	34	theme	dystroglycan	57:68	arg1	glycosylation					40:52	the functional glycosylation	25:52	the functional glycosylation of dystroglycan	25:68	Inhibitory machinery for the functional glycosylation of dystroglycan.
36760122	2	35	theme	glycosylation	338:350	arg1	patterns					352:359	unique glycosylation patterns	331:359	unique glycosylation patterns	331:359	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	8	36	from	mammals	1133:1139	arg1	present					1122:1128	present	1122:1128	present	1122:1128	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	6	37	theme	in	818:819	arg1	study					827:831	A recent in vitro study	809:831	A recent in vitro study	809:831	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	2	38	theme	unique	331:336	arg1	patterns					352:359	unique glycosylation patterns	331:359	unique glycosylation patterns	331:359	The extracellular domain of DG, α-DG, displays unique glycosylation patterns.
36760122	4	39	theme	functional	546:555	arg1	glycans					557:563	such functional glycans	541:563	such functional glycans on DG	541:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	6	40	theme	recent	811:816	arg1	study					827:831	A recent in vitro study	809:831	A recent in vitro study	809:831	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	8	41	located	present	1122:1128	arg2	CDP-Gro					1111:1117	CDP-Gro	1111:1117	CDP-Gro	1111:1117	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	8	41	located	present	1122:1128	arg1	mammals					1133:1139	mammals	1133:1139	mammals	1133:1139	Imae et al. addressed the knowledge gap regarding whether CDP-Gro is present in mammals and how CDP-Gro is synthesized and functions in mammals.
36760122	0	42	gly	glycosylation	40:52	arg1	dystroglycan					57:68	dystroglycan	57:68	dystroglycan	57:68	Inhibitory machinery for the functional glycosylation of dystroglycan.
36760122	4	43	located	found	532:536	arg1	glycans					557:563	such functional glycans	541:563	such functional glycans on DG	541:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	4	43	located	found	532:536	arg2	compositions					519:530	the unique sugar compositions	502:530	the unique sugar compositions found in such functional glycans on DG	502:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	1	44	theme	physical	231:238	arg1	strength					240:247	physical strength	231:247	physical strength	231:247	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36760122	6	45	theme	donor	964:968	arg1	CDP-glycerol					936:947	CDP-glycerol	936:947	CDP-glycerol (CDP-Gro)	936:957	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	6	45	theme	donor	964:968	arg1	substrate					970:978	a donor substrate	962:978	a donor substrate	962:978	A recent in vitro study reported that glycerol phosphate (GroP) could be similarly added to α-DG by FKTN and FKRP if they used CDP-glycerol (CDP-Gro) as a donor substrate.
36760122	3	46	theme	extracellular	452:464	arg1	matrices					466:473	extracellular matrices	452:473	extracellular matrices	452:473	Fully functional glycosylation is required for this domain to interact with components of extracellular matrices, including laminin.
36760122	4	47	theme	sugar	513:517	arg1	compositions					519:530	the unique sugar compositions	502:530	the unique sugar compositions found in such functional glycans on DG	502:569	One of the unique sugar compositions found in such functional glycans on DG is two ribitol phosphates (RboP) that are transferred by the sequential actions of fukutin (FKTN) and fukutin-related protein (FKRP), which use CDP-ribitol as a donor substrate.
36760122	1	48	theme	critical	132:139	arg1	role					141:144	a critical role	130:144	a critical role	130:144	Dystroglycan (DG), a muscular transmembrane protein, plays a critical role in transducing extracellular matrix-derived signals to the cytoskeleton and provides physical strength to skeletal muscle cell membranes.
36234897	9	0	theme	methanolic	1692:1701	arg1	extract					1703:1709	The methanolic extract	1688:1709	The methanolic extract	1688:1709	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	13	1	dep	Acarbose	2642:2649	arg1	standard					2652:2659	standard	2652:2659	standard	2652:2659	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	8	2	theme	synthetic	1664:1672	arg1	radical					1679:1685	the synthetic free radical	1660:1685	the synthetic free radical	1660:1685	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	13	3	theme	α-amylase	2743:2751	arg1	inhibition					2729:2738	85.99 ± 0.44% inhibition	2715:2738	85.99 ± 0.44% inhibition of α-amylase	2715:2751	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	13	3	theme	α-amylase	2743:2751	arg1	inhibition					2683:2692	87.65 ± 0.71% inhibition	2669:2692	87.65 ± 0.71% inhibition of α-glucosidase	2669:2709	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	11	4	theme	α-glucosidase	2038:2050	arg1	assay					2063:2067	The α-glucosidase inhibition assay	2034:2067	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL	2034:2153	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	8	5	theme	radical	1679:1685	arg1	inhibition					1646:1655	strong % inhibition	1637:1655	strong % inhibition of the synthetic free radical	1637:1685	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	5	6	theme	F6	1221:1222	arg1	presence					709:716	the presence	705:716	the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7)	705:1251	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	12	7	theme	methanolic	2378:2387	arg1	extract					2389:2395	methanolic extract	2378:2395	methanolic extract	2378:2395	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	17	8	theme	Recent	3304:3309	arg1	research					3311:3318	Recent research	3304:3318	Recent research	3304:3318	Recent research demonstrated that Cydonia oblonga may be considered an antibacterial medicinal plant.
36234897	16	9	theme	purified	3242:3249	arg1	fraction					3251:3258	purified fraction 1	3242:3260	purified fraction 1	3242:3260	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	2	10	theme	extracts	383:390	arg1	composition					296:306	the phytochemical composition	278:306	the phytochemical composition	278:306	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	2	10	theme	extracts	383:390	arg1	potentials					358:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	13	11	theme	0.44	2723:2726	arg1	±					2721:2721	±	2721:2721	±	2721:2721	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	14	12	theme	fractions	2876:2884	arg1	activities					2830:2839	all biological activities	2815:2839	all biological activities of methanolic extract and purified fractions	2815:2884	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	17	13	theme	Cydonia	3338:3344	arg1	oblonga					3346:3352	Cydonia oblonga	3338:3352	Cydonia oblonga	3338:3352	Recent research demonstrated that Cydonia oblonga may be considered an antibacterial medicinal plant.
36234897	5	14	theme	mandelic	812:819	arg1	acid					821:824	mandelic acid	812:824	mandelic acid	812:824	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	14	theme	mandelic	812:819	arg1	extract					768:774	the crude extract	758:774	the crude extract	758:774	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	10	15	theme	0.56	1975:1978	arg1	±					1973:1973	±	1973:1973	±	1973:1973	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	15	16	theme	purified	3110:3117	arg1	fractions					3119:3127	purified fractions	3110:3127	purified fractions	3110:3127	The total phenolic and flavonoid contents of methanolic extract were recorded higher as compared to purified fractions (TPC = 70% and TFC = 69%).
36234897	13	17	dep	%	2681:2681	arg1	±					2675:2675	±	2675:2675	±	2675:2675	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	12	18	theme	highest	2605:2611	arg1	concentration					2613:2625	the highest concentration	2601:2625	the highest concentration of 1000 µg/mL	2601:2639	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	2	19	theme	phytochemical	282:294	arg1	composition					296:306	the phytochemical composition	278:306	the phytochemical composition	278:306	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	10	20	with	control	1954:1960	arg1	inhibition					1981:1990	94.88 ± 0.56% inhibition	1967:1990	94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL	1967:2031	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	5	21	theme	purified	780:787	arg1	fractions					789:797	purified fractions	780:797	purified fractions	780:797	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	13	22	theme	α-glucosidase	2697:2709	arg1	inhibition					2729:2738	85.99 ± 0.44% inhibition	2715:2738	85.99 ± 0.44% inhibition of α-amylase	2715:2751	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	13	22	theme	α-glucosidase	2697:2709	arg1	inhibition					2683:2692	87.65 ± 0.71% inhibition	2669:2692	87.65 ± 0.71% inhibition of α-glucosidase	2669:2709	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	14	23	theme	biological	2819:2828	arg1	activities					2830:2839	all biological activities	2815:2839	all biological activities of methanolic extract and purified fractions	2815:2884	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	10	24	theme	94.88	1967:1971	arg1	±					1973:1973	±	1973:1973	±	1973:1973	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	11	25	theme	methanolic	2177:2186	arg1	±					2239:2239	56.10 ± 0.24	2233:2244	56.10 ± 0.24	2233:2244	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	25	theme	methanolic	2177:2186	arg1	±					2257:2257	62.44 ± 1.03	2251:2262	62.44 ± 1.03	2251:2262	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	25	theme	methanolic	2177:2186	arg1	extract					2188:2194	methanolic extract	2177:2194	methanolic extract	2177:2194	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	25	theme	methanolic	2177:2186	arg1	±					2203:2203	78.21 ± 0.67	2197:2208	78.21 ± 0.67	2197:2208	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	3	26	theme	oblonga	429:435	arg1	extraction					442:451	The Cydonia oblonga bark extraction	417:451	The Cydonia oblonga bark extraction	417:451	The Cydonia oblonga bark extraction was fractionated through HPLC and seven purified fractions labeled as F1, F2, F3, F4, F5, F6, and F7 were obtained.
36234897	4	27	theme	methanolic	593:602	arg1	extract					604:610	methanolic extract	593:610	methanolic extract	593:610	The HPLC-UV analysis of methanolic extract showed the presence of a number of possible compounds.
36234897	18	28	theme	antibiotics	3578:3588	arg1	discovery					3561:3569	the discovery	3557:3569	the discovery of new antibiotics	3557:3588	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	1	29	theme	traditional	202:212	arg1	systems					225:231	traditional medication systems	202:231	traditional medication systems	202:231	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	13	30	theme	85.99	2715:2719	arg1	±					2721:2721	±	2721:2721	±	2721:2721	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	7	31	theme	effective	1390:1398	arg1	activity					1414:1421	effective antibacterial activity	1390:1421	effective antibacterial activity	1390:1421	All the purified fractions and methanolic extract showed effective antibacterial activity; however, the highest activity was recorded for methanolic extract against Staphylococcus aureus and Streptococcus pneumonia.
36234897	12	32	dep	noted	2425:2429	arg1	extract					2446:2452	methanolic extract	2435:2452	methanolic extract	2435:2452	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	11	33	theme	methanolic	2072:2081	arg1	extract					2083:2089	methanolic extract	2072:2089	methanolic extract	2072:2089	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	4	34	theme	compounds	656:664	arg1	number					637:642	a number	635:642	a number of possible compounds	635:664	The HPLC-UV analysis of methanolic extract showed the presence of a number of possible compounds.
36234897	8	35	theme	methanolic	1575:1584	arg1	extract					1586:1592	methanolic extract	1575:1592	methanolic extract	1575:1592	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	16	36	theme	highest	3213:3219	arg1	value					3225:3229	the highest TPC value	3209:3229	the highest TPC value (64%)	3209:3235	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	16	36	theme	highest	3213:3219	arg1	%					3234:3234	64%	3232:3234	64%	3232:3234	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	18	37	used	utilized	3464:3471	arg2	it					3452:3453	it	3452:3453	it	3452:3453	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	1	38	from	number	168:173	arg1	systems					225:231	traditional medication systems	202:231	traditional medication systems	202:231	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	5	39	theme	catechin	852:859	arg1	hydrate					861:867	catechin hydrate	852:867	catechin hydrate	852:867	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	39	theme	catechin	852:859	arg1	extract					768:774	the crude extract	758:774	the crude extract	758:774	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	10	40	dep	acid	1924:1927	arg1	standard					1930:1937	standard	1930:1937	standard	1930:1937	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2591:2591	F7, 56.58 ± 0.10	2581:2596	±	2591:2591	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2551:2551	72.14 ± 0.30	2545:2556	72.14 ± 0.30	2545:2556	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2569:2569	F6, 74.24 ± 0.29	2559:2574	±	2569:2569	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2515:2515	82.16 ± 0.48	2509:2520	82.16 ± 0.48	2509:2520	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2533:2533	77.37 ± 0.28	2527:2538	77.37 ± 0.28	2527:2538	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2479:2479	79.72 ± 0.02	2473:2484	79.72 ± 0.02	2473:2484	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2497:2497	79.72 ± 0.02	2491:2502	79.72 ± 0.02	2491:2502	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	±					2461:2461	77.98 ± 0.57	2455:2466	77.98 ± 0.57	2455:2466	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	F5					2541:2542	F5	2541:2542	F5	2541:2542	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	F3					2505:2506	F3	2505:2506	F3	2505:2506	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	F4					2523:2524	F4	2523:2524	F4	2523:2524	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	F1					2469:2470	F1	2469:2470	F1	2469:2470	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	41	dep	extract	2446:2452	arg1	F2					2487:2488	F2	2487:2488	F2	2487:2488	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	11	42	theme	fractions	2104:2112	arg1	assay					2063:2067	The α-glucosidase inhibition assay	2034:2067	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL	2034:2153	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	9	43	theme	0.62	1897:1900	arg1	%					1901:1901	%	1901:1901	%	1901:1901	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	44	dep	inhibition	1735:1744	arg1	%					1733:1733	%	1733:1733	%	1733:1733	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	8	45	theme	Antioxidant	1549:1559	arg1	evaluation					1561:1570	Antioxidant evaluation	1549:1570	Antioxidant evaluation of methanolic extract and purified fractions against DPPH	1549:1628	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	7	46	theme	purified	1341:1348	arg1	fractions					1350:1358	All the purified fractions	1333:1358	All the purified fractions	1333:1358	All the purified fractions and methanolic extract showed effective antibacterial activity; however, the highest activity was recorded for methanolic extract against Staphylococcus aureus and Streptococcus pneumonia.
36234897	12	47	theme	α-Amylase	2352:2360	arg1	inhibition					2364:2373	α-Amylase % inhibition	2352:2373	α-Amylase % inhibition of methanolic extract and purified fractions	2352:2418	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	5	48	theme	F7	1249:1250	arg1	presence					709:716	the presence	705:716	the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7)	705:1251	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	11	49	theme	F4	2265:2266	arg1	inhibition					2340:2349	57.33 ± 0.05% inhibition	2326:2349	57.33 ± 0.05% inhibition	2326:2349	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	49	theme	F4	2265:2266	arg1	±					2275:2275	F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7	2265:2323	±	2275:2275	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	12	50	theme	F6	2559:2560	arg1	±					2569:2569	F6, 74.24 ± 0.29	2559:2574	±	2569:2569	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	0	51	theme	Cydonia	85:91	arg1	Bark					101:104	Cydonia oblonga Bark	85:104	Cydonia oblonga Bark	85:104	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	5	52	theme	octasiloxane	991:1002	arg1	presence					709:716	the presence	705:716	the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7)	705:1251	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	11	53	theme	±	2332:2332	arg1	inhibition					2340:2349	57.33 ± 0.05% inhibition	2326:2349	57.33 ± 0.05% inhibition	2326:2349	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	53	theme	±	2332:2332	arg1	±					2275:2275	F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7	2265:2323	±	2275:2275	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	0	54	theme	Bark	101:104	arg1	Antioxidant					42:52	Antioxidant	42:52	Antioxidant	42:52	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	0	54	theme	Bark	101:104	arg1	Potentials					71:80	Antidiabetic Potentials	58:80	Antidiabetic Potentials	58:80	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	0	54	theme	Bark	101:104	arg1	Antibacterial					27:39	Antibacterial	27:39	Antibacterial	27:39	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	0	54	theme	Bark	101:104	arg1	Composition					14:24	Phytochemical Composition	0:24	Phytochemical Composition	0:24	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	8	55	theme	strong	1637:1642	arg1	inhibition					1646:1655	strong % inhibition	1637:1655	strong % inhibition of the synthetic free radical	1637:1685	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	5	56	theme	HPLC	880:883	arg1	morin					873:877	morin	873:877	morin (HPLC analysis)	873:893	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	56	theme	HPLC	880:883	arg1	analysis					885:892	HPLC analysis	880:892	HPLC analysis	880:892	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	2	57	theme	Cydonia	395:401	arg1	bark					411:414	Cydonia oblonga bark	395:414	Cydonia oblonga bark	395:414	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	10	58	theme	Ascorbic	1915:1922	arg1	control					1954:1960	a control	1952:1960	a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL	1952:2031	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	10	58	theme	Ascorbic	1915:1922	arg1	acid					1924:1927	Ascorbic acid	1915:1927	Ascorbic acid (standard)	1915:1938	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	3	59	theme	Cydonia	421:427	arg1	extraction					442:451	The Cydonia oblonga bark extraction	417:451	The Cydonia oblonga bark extraction	417:451	The Cydonia oblonga bark extraction was fractionated through HPLC and seven purified fractions labeled as F1, F2, F3, F4, F5, F6, and F7 were obtained.
36234897	0	60	theme	Phytochemical	0:12	arg1	Composition					14:24	Phytochemical Composition	0:24	Phytochemical Composition	0:24	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	7	61	theme	highest	1437:1443	arg1	activity					1445:1452	the highest activity	1433:1452	the highest activity	1433:1452	All the purified fractions and methanolic extract showed effective antibacterial activity; however, the highest activity was recorded for methanolic extract against Staphylococcus aureus and Streptococcus pneumonia.
36234897	11	62	theme	%	2338:2338	arg1	inhibition					2340:2349	57.33 ± 0.05% inhibition	2326:2349	57.33 ± 0.05% inhibition	2326:2349	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	62	theme	%	2338:2338	arg1	±					2275:2275	F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7	2265:2323	±	2275:2275	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	14	63	theme	extract	2855:2861	arg1	activities					2830:2839	all biological activities	2815:2839	all biological activities of methanolic extract and purified fractions	2815:2884	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	14	64	theme	rich	2932:2935	arg1	they					2923:2926	they	2923:2926	they	2923:2926	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	14	64	theme	rich	2932:2935	arg1	sources					2937:2943	rich sources	2932:2943	rich sources of phenolic and flavonoids	2932:2970	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	2	65	theme	bark	411:414	arg1	extracts					383:390	methanolic extracts	372:390	methanolic extracts of Cydonia oblonga bark	372:414	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	11	66	theme	F6	2301:2302	arg1	±					2311:2311	F6, 72.68 ± 0.2	2301:2315	±	2311:2311	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	3	67	theme	purified	493:500	arg1	fractions					502:510	seven purified fractions	487:510	seven purified fractions labeled as F1, F2, F3, F4, F5, F6, and F7	487:552	The Cydonia oblonga bark extraction was fractionated through HPLC and seven purified fractions labeled as F1, F2, F3, F4, F5, F6, and F7 were obtained.
36234897	5	68	theme	F3	1038:1039	arg1	presence					709:716	the presence	705:716	the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7)	705:1251	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	69	theme	diisooctyl	928:937	arg1	phthalate					939:947	diisooctyl phthalate	928:947	diisooctyl phthalate	928:947	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	69	theme	diisooctyl	928:937	arg1	morin					873:877	morin	873:877	morin (HPLC analysis)	873:893	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	15	70	theme	methanolic	3055:3064	arg1	extract					3066:3072	methanolic extract	3055:3072	methanolic extract	3055:3072	The total phenolic and flavonoid contents of methanolic extract were recorded higher as compared to purified fractions (TPC = 70% and TFC = 69%).
36234897	12	71	theme	fractions	2410:2418	arg1	inhibition					2364:2373	α-Amylase % inhibition	2352:2373	α-Amylase % inhibition of methanolic extract and purified fractions	2352:2418	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	8	72	theme	fractions	1607:1615	arg1	evaluation					1561:1570	Antioxidant evaluation	1549:1570	Antioxidant evaluation of methanolic extract and purified fractions against DPPH	1549:1628	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	1	73	theme	medication	214:223	arg1	systems					225:231	traditional medication systems	202:231	traditional medication systems	202:231	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	14	74	theme	bioactive	2989:2997	arg1	compounds					2999:3007	other bioactive compounds	2983:3007	other bioactive compounds	2983:3007	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	5	75	theme	BIS-	896:899	arg1	F1					950:951	F1	950:951	F1	950:951	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	75	theme	BIS-	896:899	arg1	phthalate					914:922	BIS-(2-ethylhexyl)phthalate	896:922	BIS-(2-ethylhexyl)phthalate	896:922	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	75	theme	BIS-	896:899	arg1	morin					873:877	morin	873:877	morin (HPLC analysis)	873:893	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	76	dep	F2	966:967	arg1	azule					1129:1133	azule	1129:1133	azule	1129:1133	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	76	dep	F2	966:967	arg1	6					1111:1111	6	1111:1111	6	1111:1111	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	76	dep	F2	966:967	arg1	2					1122:1122	2	1122:1122	2	1122:1122	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	76	dep	F2	966:967	arg1	acid					1195:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid	1140:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5)	1140:1203	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	76	dep	F2	966:967	arg1	benz[1					1114:1119	benz[1	1114:1119	benz[1	1114:1119	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	76	dep	F2	966:967	arg1	F5					1201:1202	F5	1201:1202	F5	1201:1202	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	10	77	used	used	1944:1947	arg2	acid					1924:1927	Ascorbic acid	1915:1927	Ascorbic acid (standard)	1915:1938	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	10	77	used	used	1944:1947	arg2	control					1954:1960	a control	1952:1960	a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL	1952:2031	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	17	78	theme	medicinal	3389:3397	arg1	plant					3399:3403	an antibacterial medicinal plant	3372:3403	an antibacterial medicinal plant	3372:3403	Recent research demonstrated that Cydonia oblonga may be considered an antibacterial medicinal plant.
36234897	5	79	theme	bioactive	735:743	arg1	compounds					745:753	bioactive compounds	735:753	bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2	735:967	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	80	theme	2-ethylhexyl	901:912	arg1	F1					950:951	F1	950:951	F1	950:951	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	80	theme	2-ethylhexyl	901:912	arg1	phthalate					914:922	BIS-(2-ethylhexyl)phthalate	896:922	BIS-(2-ethylhexyl)phthalate	896:922	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	80	theme	2-ethylhexyl	901:912	arg1	morin					873:877	morin	873:877	morin (HPLC analysis)	873:893	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	18	81	theme	study	3432:3436	arg1	result					3410:3415	The result	3406:3415	The result of the present study	3406:3436	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	8	82	theme	free	1674:1677	arg1	radical					1679:1685	the synthetic free radical	1660:1685	the synthetic free radical	1660:1685	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	2	83	theme	ant-diabetic	345:356	arg1	potentials					358:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	13	84	theme	µg/mL	2790:2794	arg1	concentration					2768:2780	the highest concentration	2756:2780	the highest concentration of 1000 µg/mL	2756:2794	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	5	85	dep	extract	768:774	arg1	acid					806:809	malic acid	800:809	malic acid	800:809	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	13	86	dep	inhibition	2729:2738	arg1	%					2727:2727	%	2727:2727	%	2727:2727	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	8	87	theme	purified	1598:1605	arg1	fractions					1607:1615	purified fractions	1598:1615	purified fractions against DPPH	1598:1628	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	5	88	theme	F4	1078:1079	arg1	presence					709:716	the presence	705:716	the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7)	705:1251	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	18	89	theme	bioactive	3494:3502	arg1	phytochemicals					3504:3517	bioactive phytochemicals	3494:3517	bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics	3494:3588	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	1	90	used	used	152:155	arg2	oblonga					115:121	Cydonia oblonga	107:121	Cydonia oblonga	107:121	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	1	90	used	used	152:155	arg2	plant					138:142	a medicinal plant	126:142	a medicinal plant that is used to treat a number of health complications in traditional medication systems	126:231	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	9	91	theme	87.41	1721:1725	arg1	±					1727:1727	±	1727:1727	±	1727:1727	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	5	92	theme	malic	800:804	arg1	acid					806:809	malic acid	800:809	malic acid	800:809	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	13	93	theme	highest	2760:2766	arg1	concentration					2768:2780	the highest concentration	2756:2780	the highest concentration of 1000 µg/mL	2756:2794	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	9	94	theme	0.54	1729:1732	arg1	±					1727:1727	±	1727:1727	±	1727:1727	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	4	95	theme	extract	604:610	arg1	analysis					581:588	The HPLC-UV analysis	569:588	The HPLC-UV analysis of methanolic extract	569:610	The HPLC-UV analysis of methanolic extract showed the presence of a number of possible compounds.
36234897	13	96	dep	inhibition	2683:2692	arg1	%					2681:2681	%	2681:2681	%	2681:2681	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	9	97	dep	F1	1772:1773	arg1	±					1800:1800	65.78 ± 0.68	1794:1805	65.78 ± 0.68	1794:1805	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	±					1818:1818	58.61 ± 0.58	1812:1823	58.61 ± 0.58	1812:1823	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	F6					1863:1864	F6	1863:1864	F6	1863:1864	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	F4					1826:1827	F4	1826:1827	F4	1826:1827	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	F7					1885:1886	F7	1885:1886	F7	1885:1886	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	F5					1844:1845	F5	1844:1845	F5	1844:1845	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	F2					1790:1791	F2	1790:1791	F2	1790:1791	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	F3					1808:1809	F3	1808:1809	F3	1808:1809	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	±					1873:1873	85.28 ± 0.94	1867:1878	85.28 ± 0.94	1867:1878	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	±					1836:1836	80.76 ± 0.59	1830:1841	80.76 ± 0.59	1830:1841	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	9	97	dep	F1	1772:1773	arg1	±					1855:1855	571.29 ± 0.49	1848:1860	571.29 ± 0.49	1848:1860	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	18	98	theme	present	3424:3430	arg1	study					3432:3436	the present study	3420:3436	the present study	3420:3436	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	16	99	theme	highest	3276:3282	arg1	value					3284:3288	the highest value	3272:3288	the highest value of TFC	3272:3295	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	14	100	theme	purified	2867:2874	arg1	fractions					2876:2884	purified fractions	2867:2884	purified fractions	2867:2884	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	6	101	theme	purified	1270:1277	arg1	fractions					1279:1287	purified fractions	1270:1287	purified fractions	1270:1287	The extract and purified fractions were then tested for biological activities.
36234897	1	102	theme	health	178:183	arg1	complications					185:197	health complications	178:197	health complications	178:197	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	7	103	theme	antibacterial	1400:1412	arg1	activity					1414:1421	effective antibacterial activity	1390:1421	effective antibacterial activity	1390:1421	All the purified fractions and methanolic extract showed effective antibacterial activity; however, the highest activity was recorded for methanolic extract against Staphylococcus aureus and Streptococcus pneumonia.
36234897	10	104	from	concentration	2005:2017	arg1	inhibition					1981:1990	94.88 ± 0.56% inhibition	1967:1990	94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL	1967:2031	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	4	105	theme	HPLC-UV	573:579	arg1	analysis					581:588	The HPLC-UV analysis	569:588	The HPLC-UV analysis of methanolic extract	569:610	The HPLC-UV analysis of methanolic extract showed the presence of a number of possible compounds.
36234897	12	106	theme	extract	2389:2395	arg1	inhibition					2364:2373	α-Amylase % inhibition	2352:2373	α-Amylase % inhibition of methanolic extract and purified fractions	2352:2418	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	107	theme	F7	2581:2582	arg1	±					2591:2591	F7, 56.58 ± 0.10	2581:2596	±	2591:2591	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	5	108	theme	F2	966:967	arg1	presence					709:716	the presence	705:716	the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7)	705:1251	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	9	109	dep	%	1733:1733	arg1	±					1727:1727	±	1727:1727	±	1727:1727	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	5	110	theme	caffeic	838:844	arg1	acid					846:849	caffeic acid	838:849	caffeic acid	838:849	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	110	theme	caffeic	838:844	arg1	extract					768:774	the crude extract	758:774	the crude extract	758:774	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	11	111	from	concentration	2127:2139	arg1	assay					2063:2067	The α-glucosidase inhibition assay	2034:2067	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL	2034:2153	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	7	112	theme	methanolic	1364:1373	arg1	extract					1375:1381	methanolic extract	1364:1381	methanolic extract	1364:1381	All the purified fractions and methanolic extract showed effective antibacterial activity; however, the highest activity was recorded for methanolic extract against Staphylococcus aureus and Streptococcus pneumonia.
36234897	11	113	theme	78.21	2197:2201	arg1	extract					2188:2194	methanolic extract	2177:2194	methanolic extract	2177:2194	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	113	theme	78.21	2197:2201	arg1	±					2203:2203	78.21 ± 0.67	2197:2208	78.21 ± 0.67	2197:2208	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	114	theme	inhibition	2052:2061	arg1	assay					2063:2067	The α-glucosidase inhibition assay	2034:2067	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL	2034:2153	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	4	115	theme	possible	647:654	arg1	compounds					656:664	possible compounds	647:664	possible compounds	647:664	The HPLC-UV analysis of methanolic extract showed the presence of a number of possible compounds.
36234897	18	116	from	opportunities	3540:3552	arg1	discovery					3561:3569	the discovery	3557:3569	the discovery of new antibiotics	3557:3588	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	2	117	theme	study	256:260	arg1	objective					238:246	The objective	234:246	The objective of this study	234:260	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	1	118	theme	medicinal	128:136	arg1	plant					138:142	a medicinal plant	126:142	a medicinal plant that is used to treat a number of health complications in traditional medication systems	126:231	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	1	118	theme	medicinal	128:136	arg1	oblonga					115:121	Cydonia oblonga	107:121	Cydonia oblonga	107:121	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	18	119	theme	new	3574:3576	arg1	antibiotics					3578:3588	new antibiotics	3574:3588	new antibiotics	3574:3588	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	11	120	dep	activities	2162:2171	arg1	±					2293:2293	62.18 ± 0.92	2287:2298	62.18 ± 0.92	2287:2298	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	±					2239:2239	56.10 ± 0.24	2233:2244	56.10 ± 0.24	2233:2244	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	±					2257:2257	62.44 ± 1.03	2251:2262	62.44 ± 1.03	2251:2262	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	±					2203:2203	78.21 ± 0.67	2197:2208	78.21 ± 0.67	2197:2208	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	F7					2322:2323	F7	2322:2323	F7	2322:2323	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	F5					2283:2284	F5	2283:2284	F5	2283:2284	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	inhibition					2340:2349	57.33 ± 0.05% inhibition	2326:2349	57.33 ± 0.05% inhibition	2326:2349	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	±					2311:2311	F6, 72.68 ± 0.2	2301:2315	±	2311:2311	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	extract					2188:2194	methanolic extract	2177:2194	methanolic extract	2177:2194	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	120	dep	activities	2162:2171	arg1	±					2275:2275	F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7	2265:2323	±	2275:2275	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	14	121	theme	other	2983:2987	arg1	compounds					2999:3007	other bioactive compounds	2983:3007	other bioactive compounds	2983:3007	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	11	122	theme	purified	2095:2102	arg1	fractions					2104:2112	purified fractions	2095:2112	purified fractions	2095:2112	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	8	123	theme	extract	1586:1592	arg1	evaluation					1561:1570	Antioxidant evaluation	1549:1570	Antioxidant evaluation of methanolic extract and purified fractions against DPPH	1549:1628	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	16	124	theme	TPC	3221:3223	arg1	value					3225:3229	the highest TPC value	3209:3229	the highest TPC value (64%)	3209:3235	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	16	124	theme	TPC	3221:3223	arg1	%					3234:3234	64%	3232:3234	64%	3232:3234	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	11	125	theme	maximum	2119:2125	arg1	concentration					2127:2139	a maximum concentration	2117:2139	a maximum concentration of 1000 µg/mL	2117:2153	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	126	theme	extract	2083:2089	arg1	assay					2063:2067	The α-glucosidase inhibition assay	2034:2067	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL	2034:2153	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	9	127	theme	%	1901:1901	arg1	inhibition					1903:1912	0.62% inhibition	1897:1912	0.62% inhibition	1897:1912	The methanolic extract exhibited 87.41 ± 0.54% inhibition whereas fractions showed: F1, 85.45 ± 0.85; F2, 65.78 ± 0.68; F3, 58.61 ± 0.58; F4, 80.76 ± 0.59; F5, 571.29 ± 0.49; F6, 85.28 ± 0.94; and F7, 48.45 ± 0.62% inhibition.
36234897	0	128	theme	oblonga	93:99	arg1	Bark					101:104	Cydonia oblonga Bark	85:104	Cydonia oblonga Bark	85:104	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	5	129	from	compounds	745:753	arg1	acid					846:849	caffeic acid	838:849	caffeic acid	838:849	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	129	from	compounds	745:753	arg1	fractions					789:797	purified fractions	780:797	purified fractions	780:797	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	129	from	compounds	745:753	arg1	acid					821:824	mandelic acid	812:824	mandelic acid	812:824	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	129	from	compounds	745:753	arg1	quercetin					827:835	quercetin	827:835	quercetin	827:835	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	129	from	compounds	745:753	arg1	hydrate					861:867	catechin hydrate	852:867	catechin hydrate	852:867	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	129	from	compounds	745:753	arg1	extract					768:774	the crude extract	758:774	the crude extract	758:774	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	13	130	theme	0.71	2677:2680	arg1	±					2675:2675	±	2675:2675	±	2675:2675	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	6	131	theme	biological	1310:1319	arg1	activities					1321:1330	biological activities	1310:1330	biological activities	1310:1330	The extract and purified fractions were then tested for biological activities.
36234897	11	132	dep	extract	2188:2194	arg1	±					2221:2221	F1, 55.01 ± 0.29	2211:2226	±	2221:2221	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	132	dep	extract	2188:2194	arg1	F2					2229:2230	F2	2229:2230	F2	2229:2230	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	132	dep	extract	2188:2194	arg1	F3					2247:2248	F3	2247:2248	F3	2247:2248	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	10	133	theme	maximum	1997:2003	arg1	concentration					2005:2017	a maximum concentration	1995:2017	a maximum concentration of 1000 µg/mL	1995:2031	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	4	134	theme	number	637:642	arg1	presence					623:630	the presence	619:630	the presence of a number of possible compounds	619:664	The HPLC-UV analysis of methanolic extract showed the presence of a number of possible compounds.
36234897	5	135	theme	GC-MS	671:675	arg1	analysis					686:693	The GC-MS and HPLC analysis	667:693	analysis	686:693	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	12	136	from	concentration	2613:2625	arg1	±					2591:2591	F7, 56.58 ± 0.10	2581:2596	±	2591:2591	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	136	from	concentration	2613:2625	arg1	±					2551:2551	72.14 ± 0.30	2545:2556	72.14 ± 0.30	2545:2556	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	136	from	concentration	2613:2625	arg1	±					2569:2569	F6, 74.24 ± 0.29	2559:2574	±	2569:2569	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	11	137	theme	µg/mL	2149:2153	arg1	concentration					2127:2139	a maximum concentration	2117:2139	a maximum concentration of 1000 µg/mL	2117:2153	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	13	138	dep	%	2727:2727	arg1	±					2721:2721	±	2721:2721	±	2721:2721	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	16	139	dep	exhibited	3262:3270	arg1	%					3300:3300	58%	3298:3300	58%	3298:3300	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	15	140	theme	flavonoid	3033:3041	arg1	contents					3043:3050	The total phenolic and flavonoid contents	3010:3050	contents	3043:3050	The total phenolic and flavonoid contents of methanolic extract were recorded higher as compared to purified fractions (TPC = 70% and TFC = 69%).
36234897	12	141	theme	purified	2401:2408	arg1	fractions					2410:2418	purified fractions	2401:2418	purified fractions	2401:2418	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	5	142	theme	HPLC	681:684	arg1	analysis					686:693	The GC-MS and HPLC analysis	667:693	analysis	686:693	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	1	143	theme	complications	185:197	arg1	number					168:173	a number	166:173	a number of health complications in traditional medication systems	166:231	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	7	144	theme	methanolic	1471:1480	arg1	extract					1482:1488	methanolic extract	1471:1488	methanolic extract against Staphylococcus aureus and Streptococcus pneumonia	1471:1546	All the purified fractions and methanolic extract showed effective antibacterial activity; however, the highest activity was recorded for methanolic extract against Staphylococcus aureus and Streptococcus pneumonia.
36234897	10	145	theme	µg/mL	2027:2031	arg1	concentration					2005:2017	a maximum concentration	1995:2017	a maximum concentration of 1000 µg/mL	1995:2031	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	11	146	theme	57.33	2326:2330	arg1	inhibition					2340:2349	57.33 ± 0.05% inhibition	2326:2349	57.33 ± 0.05% inhibition	2326:2349	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	146	theme	57.33	2326:2330	arg1	±					2275:2275	F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7	2265:2323	±	2275:2275	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	12	147	theme	methanolic	2435:2444	arg1	extract					2446:2452	methanolic extract	2435:2452	methanolic extract	2435:2452	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	13	148	theme	87.65	2669:2673	arg1	±					2675:2675	±	2675:2675	±	2675:2675	Acarbose (standard) showed 87.65 ± 0.71% inhibition of α-glucosidase and 85.99 ± 0.44% inhibition of α-amylase at the highest concentration of 1000 µg/mL.
36234897	8	149	theme	%	1644:1644	arg1	inhibition					1646:1655	strong % inhibition	1637:1655	strong % inhibition of the synthetic free radical	1637:1685	Antioxidant evaluation of methanolic extract and purified fractions against DPPH showed strong % inhibition of the synthetic free radical.
36234897	16	150	theme	purified	3168:3175	arg1	fractions					3177:3185	the purified fractions	3164:3185	the purified fractions	3164:3185	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	11	151	theme	56.10	2233:2237	arg1	±					2239:2239	56.10 ± 0.24	2233:2244	56.10 ± 0.24	2233:2244	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	11	151	theme	56.10	2233:2237	arg1	extract					2188:2194	methanolic extract	2177:2194	methanolic extract	2177:2194	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	14	152	theme	phenolic	2948:2955	arg1	they					2923:2926	they	2923:2926	they	2923:2926	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	14	152	theme	phenolic	2948:2955	arg1	sources					2937:2943	rich sources	2932:2943	rich sources of phenolic and flavonoids	2932:2970	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	2	153	theme	methanolic	372:381	arg1	extracts					383:390	methanolic extracts	372:390	methanolic extracts of Cydonia oblonga bark	372:414	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	5	154	theme	crude	762:766	arg1	quercetin					827:835	quercetin	827:835	quercetin	827:835	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	154	theme	crude	762:766	arg1	acid					846:849	caffeic acid	838:849	caffeic acid	838:849	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	154	theme	crude	762:766	arg1	hydrate					861:867	catechin hydrate	852:867	catechin hydrate	852:867	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	154	theme	crude	762:766	arg1	acid					821:824	mandelic acid	812:824	mandelic acid	812:824	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	154	theme	crude	762:766	arg1	extract					768:774	the crude extract	758:774	the crude extract	758:774	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	155	theme	silicic	1043:1049	arg1	acid					1051:1054	silicic acid	1043:1054	silicic acid	1043:1054	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	155	theme	silicic	1043:1049	arg1	F2					966:967	F2	966:967	F2	966:967	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	18	156	theme	phytochemicals	3504:3517	arg1	isolation					3481:3489	the isolation	3477:3489	the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics	3477:3588	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	1	157	theme	Cydonia	107:113	arg1	plant					138:142	a medicinal plant	126:142	a medicinal plant that is used to treat a number of health complications in traditional medication systems	126:231	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	1	157	theme	Cydonia	107:113	arg1	oblonga					115:121	Cydonia oblonga	107:121	Cydonia oblonga	107:121	Cydonia oblonga is a medicinal plant that is used to treat a number of health complications in traditional medication systems.
36234897	12	158	theme	72.14	2545:2549	arg1	±					2551:2551	72.14 ± 0.30	2545:2556	72.14 ± 0.30	2545:2556	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	12	159	theme	µg/mL	2635:2639	arg1	concentration					2613:2625	the highest concentration	2601:2625	the highest concentration of 1000 µg/mL	2601:2639	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
36234897	14	160	theme	methanolic	2844:2853	arg1	extract					2855:2861	methanolic extract	2844:2861	methanolic extract	2844:2861	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	18	161	theme	new	3536:3538	arg1	opportunities					3540:3552	new opportunities	3536:3552	new opportunities in the discovery of new antibiotics	3536:3588	The result of the present study revealed that it might be utilized for the isolation of bioactive phytochemicals that can lead to new opportunities in the discovery of new antibiotics.
36234897	3	162	theme	bark	437:440	arg1	extraction					442:451	The Cydonia oblonga bark extraction	417:451	The Cydonia oblonga bark extraction	417:451	The Cydonia oblonga bark extraction was fractionated through HPLC and seven purified fractions labeled as F1, F2, F3, F4, F5, F6, and F7 were obtained.
36234897	5	163	theme	-3-morpholinepyrol-2-yl	1158:1180	arg1	F5					1201:1202	F5	1201:1202	F5	1201:1202	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	163	theme	-3-morpholinepyrol-2-yl	1158:1180	arg1	acid					1195:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid	1140:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5)	1140:1203	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	2	164	theme	oblonga	403:409	arg1	bark					411:414	Cydonia oblonga bark	395:414	Cydonia oblonga bark	395:414	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	0	165	theme	Antidiabetic	58:69	arg1	Potentials					71:80	Antidiabetic Potentials	58:80	Antidiabetic Potentials	58:80	Phytochemical Composition, Antibacterial, Antioxidant and Antidiabetic Potentials of Cydonia oblonga Bark.
36234897	10	166	dep	%	1979:1979	arg1	±					1973:1973	±	1973:1973	±	1973:1973	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	5	167	theme	-butenedioic	1182:1193	arg1	F5					1201:1202	F5	1201:1202	F5	1201:1202	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	167	theme	-butenedioic	1182:1193	arg1	acid					1195:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid	1140:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5)	1140:1203	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	2	168	theme	antioxidant	328:338	arg1	potentials					358:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	16	169	theme	TFC	3293:3295	arg1	value					3284:3288	the highest value	3272:3288	the highest value of TFC	3272:3295	Amongst the purified fractions, fraction 6 exhibited the highest TPC value (64%), and purified fraction 1 exhibited the highest value of TFC (58%).
36234897	11	170	theme	F1	2211:2212	arg1	±					2221:2221	F1, 55.01 ± 0.29	2211:2226	±	2221:2221	The α-glucosidase inhibition assay of methanolic extract and purified fractions at a maximum concentration of 1000 µg/mL showed activities as: methanolic extract, 78.21 ± 0.67; F1, 55.01 ± 0.29; F2, 56.10 ± 0.24; F3, 62.44 ± 1.03; F4, 70.52 ± 0.15; F5, 62.18 ± 0.92; F6, 72.68 ± 0.2; and F7, 57.33 ± 0.05% inhibition.
36234897	14	171	theme	flavonoids	2961:2970	arg1	they					2923:2926	they	2923:2926	they	2923:2926	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	14	171	theme	flavonoids	2961:2970	arg1	sources					2937:2943	rich sources	2932:2943	rich sources of phenolic and flavonoids	2932:2970	It was found that all biological activities of methanolic extract and purified fractions might be attributed to the fact that they are rich sources of phenolic and flavonoids along with other bioactive compounds.
36234897	15	172	theme	extract	3066:3072	arg1	phenolic					3020:3027	phenolic	3020:3027	phenolic	3020:3027	The total phenolic and flavonoid contents of methanolic extract were recorded higher as compared to purified fractions (TPC = 70% and TFC = 69%).
36234897	2	173	theme	antibacterial	313:325	arg1	potentials					358:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	antibacterial, antioxidant, and ant-diabetic potentials	313:367	The objective of this study was to evaluate the phytochemical composition, and antibacterial, antioxidant, and ant-diabetic potentials of methanolic extracts of Cydonia oblonga bark.
36234897	17	174	theme	antibacterial	3375:3387	arg1	plant					3399:3403	an antibacterial medicinal plant	3372:3403	an antibacterial medicinal plant	3372:3403	Recent research demonstrated that Cydonia oblonga may be considered an antibacterial medicinal plant.
36234897	10	175	dep	inhibition	1981:1990	arg1	%					1979:1979	%	1979:1979	%	1979:1979	Ascorbic acid (standard) was used as a control with 94.88 ± 0.56% inhibition at a maximum concentration of 1000 µg/mL.
36234897	5	176	theme	4-chlorophenyl	1143:1156	arg1	F5					1201:1202	F5	1201:1202	F5	1201:1202	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	5	176	theme	4-chlorophenyl	1143:1156	arg1	acid					1195:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid	1140:1198	4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5)	1140:1203	The GC-MS and HPLC analysis confirmed the presence of the following bioactive compounds in the crude extract and purified fractions: malic acid, mandelic acid, quercetin, caffeic acid, catechin hydrate, as morin (HPLC analysis), BIS-(2-ethylhexyl)phthalate and diisooctyl phthalate (F1), carbamide (F2, used as fertilizer), octasiloxane and dimethylsiloxanecyclictrimer (F3), silicic acid and cyclotrisiloxane (F4), 6-AH-cAMP, 4H-cyclopropa[5',6']benz[1',2',7,8]azule, and 4-(4-chlorophenyl)-3-morpholinepyrol-2-yl)-butenedioic acid (F5), isopropyamine (F6), and 1-propylhydrazine (F7).
36234897	12	177	theme	%	2362:2362	arg1	inhibition					2364:2373	α-Amylase % inhibition	2352:2373	α-Amylase % inhibition of methanolic extract and purified fractions	2352:2418	α-Amylase % inhibition of methanolic extract and purified fractions were noted as: methanolic extract, 77.98 ± 0.57; F1, 79.72 ± 0.02; F2, 79.72 ± 0.02; F3, 82.16 ± 0.48; F4, 77.37 ± 0.28; F5, 72.14 ± 0.30; F6, 74.24 ± 0.29; and F7, 56.58 ± 0.10 at the highest concentration of 1000 µg/mL.
35845609	0	0	theme	Han	82:84	arg1	Nationality					86:96	Han Nationality	82:96	Han Nationality	82:96	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	6	1	theme	glycans	738:744	arg1	%					776:776	93.72%	771:776	93.72%	771:776	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	6	1	theme	glycans	738:744	arg1	proportion					712:721	The proportion	708:721	The proportion of fucosylated glycans in total IgG glycans	708:765	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	7	2	theme	monogalactosylated	898:915	arg1	glycans					938:944	total monogalactosylated and digalactosylated glycans	892:944	total monogalactosylated and digalactosylated glycans	892:944	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	8	3	theme	bisected	1118:1125	arg1	glycans					1134:1140	bisected GlcNAc glycans	1118:1140	bisected GlcNAc glycans	1118:1140	There was no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans.
35845609	0	4	from	Profile	11:17	arg1	Children					56:63	Children	56:63	Children With Epilepsy in Han Nationality	56:96	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	5	5	theme	interaction	606:616	arg1	chromatography					625:638	hydrophilic interaction liquid chromatography	594:638	hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach	594:697	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	8	6	from	groups	1108:1113	arg1	glycans					1134:1140	bisected GlcNAc glycans	1118:1140	bisected GlcNAc glycans	1118:1140	There was no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans.
35845609	8	6	from	groups	1108:1113	arg1	glycans					1157:1163	sialylated glycans	1146:1163	sialylated glycans	1146:1163	There was no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans.
35845609	9	7	theme	core	1193:1196	arg1	fucosylation					1198:1209	core fucosylation	1193:1209	core fucosylation	1193:1209	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	3	8	gly	glycosylation	405:417	arg1	children					430:437	children	430:437	children with epilepsy	430:451	Here we analyzed the characteristics of the immunoglobulin glycosylation profile of children with epilepsy.
35845609	5	9	theme	liquid	618:623	arg1	chromatography					625:638	hydrophilic interaction liquid chromatography	594:638	hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach	594:697	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	6	10	dep	Results	700:706	arg1	%					776:776	93.72%	771:776	93.72%	771:776	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	6	10	dep	Results	700:706	arg1	proportion					712:721	The proportion	708:721	The proportion of fucosylated glycans in total IgG glycans	708:765	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	7	11	theme	patients	976:983	arg1	%					1007:1007	30.76 and 40.14%	992:1007	30.76 and 40.14%	992:1007	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	7	11	theme	patients	976:983	arg1	group					985:989	the epilepsy patients group	963:989	the epilepsy patients group (30.76 and 40.14%)	963:1008	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	5	12	theme	ultra-high-performance	645:666	arg1	chromatography					675:688	ultra-high-performance liquid chromatography	645:688	ultra-high-performance liquid chromatography approach	645:697	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	2	13	theme	G	278:278	arg1	glycosylation					286:298	immunoglobulin G (IgG) glycosylation	263:298	immunoglobulin G (IgG) glycosylation	263:298	Studies suggested that immunoglobulin G (IgG) glycosylation are closely related to neurological diseases.
35845609	7	14	theme	lower	877:881	arg1	level					883:887	A lower level	875:887	A lower level of total monogalactosylated and digalactosylated glycans	875:944	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	8	15	theme	sialylated	1146:1155	arg1	glycans					1157:1163	sialylated glycans	1146:1163	sialylated glycans	1146:1163	There was no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans.
35845609	0	16	theme	Glycosylation	39:51	arg1	Profile					11:17	Expression Profile	0:17	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.	0:97	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	2	17	theme	immunoglobulin	263:276	arg1	IgG					281:283	IgG	281:283	IgG	281:283	Studies suggested that immunoglobulin G (IgG) glycosylation are closely related to neurological diseases.
35845609	2	17	theme	immunoglobulin	263:276	arg1	G					278:278	immunoglobulin G	263:278	immunoglobulin G (IgG) glycosylation	263:298	Studies suggested that immunoglobulin G (IgG) glycosylation are closely related to neurological diseases.
35845609	6	18	theme	IgG	755:757	arg1	glycans					759:765	total IgG glycans	749:765	total IgG glycans	749:765	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	9	19	theme	epilepsy	1318:1325	arg1	occurrence					1304:1313	the occurrence	1300:1313	the occurrence of epilepsy in children	1300:1337	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	5	20	theme	chromatography	675:688	arg1	approach					690:697	ultra-high-performance liquid chromatography approach	645:697	ultra-high-performance liquid chromatography approach	645:697	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	7	21	theme	epilepsy	967:974	arg1	patients					976:983	epilepsy patients	967:983	the epilepsy patients group (30.76 and 40.14%)	963:1008	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	6	22	theme	total	749:753	arg1	glycans					759:765	total IgG glycans	749:765	total IgG glycans	749:765	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	3	23	theme	children	430:437	arg1	profile					419:425	the immunoglobulin glycosylation profile	386:425	the immunoglobulin glycosylation profile of children with epilepsy	386:451	Here we analyzed the characteristics of the immunoglobulin glycosylation profile of children with epilepsy.
35845609	0	24	theme	Expression	0:9	arg1	Profile					11:17	Expression Profile	0:17	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.	0:97	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	9	25	theme	fucosylation	1198:1209	arg1	decrease					1181:1188	The decrease	1177:1188	The decrease of core fucosylation and galactosylation	1177:1229	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	8	26	theme	GlcNAc	1127:1132	arg1	glycans					1134:1140	bisected GlcNAc glycans	1118:1140	bisected GlcNAc glycans	1118:1140	There was no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans.
35845609	1	27	theme	more	180:183	arg1	half					190:193	more than half	180:193	more than half of adult epilepsy	180:211	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	0	28	theme	Immunoglobulin	22:35	arg1	Glycosylation					39:51	Immunoglobulin G Glycosylation	22:51	Immunoglobulin G Glycosylation	22:51	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	5	29	theme	hydrophilic	594:604	arg1	chromatography					625:638	hydrophilic interaction liquid chromatography	594:638	hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach	594:697	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	1	30	theme	Background	99:108	arg1	disease					138:144	a chronic brain disease	122:144	a chronic brain disease that recurs during childhood	122:173	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	1	30	theme	Background	99:108	arg1	Epilepsy					110:117	Background Epilepsy	99:117	Background Epilepsy	99:117	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	6	31	theme	control	851:857	arg1	group					859:863	the control group	847:863	the control group (94.94%)	847:872	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	6	31	theme	control	851:857	arg1	%					871:871	94.94%	866:871	94.94%	866:871	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	1	32	theme	than	185:188	arg1	half					190:193	more than half	180:193	more than half of adult epilepsy	180:211	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	7	33	located	observed	951:958	arg1	%					1007:1007	30.76 and 40.14%	992:1007	30.76 and 40.14%	992:1007	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	7	33	located	observed	951:958	arg1	group					985:989	the epilepsy patients group	963:989	the epilepsy patients group (30.76 and 40.14%)	963:1008	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	7	33	located	observed	951:958	arg2	level					883:887	A lower level	875:887	A lower level of total monogalactosylated and digalactosylated glycans	875:944	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	9	34	theme	galactosylation	1215:1229	arg1	decrease					1181:1188	The decrease	1177:1188	The decrease of core fucosylation and galactosylation	1177:1229	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	6	35	theme	fucosylated	726:736	arg1	glycans					738:744	fucosylated glycans	726:744	fucosylated glycans	726:744	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	3	36	theme	profile	419:425	arg1	characteristics					367:381	the characteristics	363:381	the characteristics of the immunoglobulin glycosylation profile of children with epilepsy	363:451	Here we analyzed the characteristics of the immunoglobulin glycosylation profile of children with epilepsy.
35845609	8	37	theme	significant	1069:1079	arg1	difference					1081:1090	no significant difference	1066:1090	no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans	1066:1163	There was no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans.
35845609	4	38	theme	Methods	454:460	arg1	Patients					462:469	Methods Patients	454:469	Methods Patients	454:469	Methods Patients were recruited in Taian, Shandong Province from December 2019 to March 2020.
35845609	9	39	dep	Conclusion	1166:1175	arg1	participate					1285:1295	participate	1285:1295	participate in the occurrence of epilepsy in children	1285:1337	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	9	39	dep	Conclusion	1166:1175	arg1	promote					1235:1241	promote	1235:1241	promote the inflammatory reaction of the body	1235:1279	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	0	40	theme	G	37:37	arg1	Glycosylation					39:51	Immunoglobulin G Glycosylation	22:51	Immunoglobulin G Glycosylation	22:51	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	5	41	with	chromatography	625:638	arg1	approach					690:697	ultra-high-performance liquid chromatography approach	645:697	ultra-high-performance liquid chromatography approach	645:697	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	0	42	from	Epilepsy	70:77	arg1	Nationality					86:96	Han Nationality	82:96	Han Nationality	82:96	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	5	43	theme	Serum	548:552	arg1	composition					566:576	Serum IgG glycome composition	548:576	Serum IgG glycome composition	548:576	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	1	44	theme	adult	198:202	arg1	epilepsy					204:211	adult epilepsy	198:211	adult epilepsy	198:211	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	3	45	theme	immunoglobulin	390:403	arg1	profile					419:425	the immunoglobulin glycosylation profile	386:425	the immunoglobulin glycosylation profile of children with epilepsy	386:451	Here we analyzed the characteristics of the immunoglobulin glycosylation profile of children with epilepsy.
35845609	4	46	dep	December	519:526	arg1	to					533:534	to	533:534	to	533:534	Methods Patients were recruited in Taian, Shandong Province from December 2019 to March 2020.
35845609	4	46	dep	December	519:526	arg1	March					536:540	March	536:540	March	536:540	Methods Patients were recruited in Taian, Shandong Province from December 2019 to March 2020.
35845609	3	47	with	children	430:437	arg1	epilepsy					444:451	epilepsy	444:451	epilepsy	444:451	Here we analyzed the characteristics of the immunoglobulin glycosylation profile of children with epilepsy.
35845609	6	48	gly	fucosylated	726:736	arg1	glycans					738:744	fucosylated glycans	726:744	fucosylated glycans	726:744	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	6	49	from	proportion	712:721	arg1	glycans					759:765	total IgG glycans	749:765	total IgG glycans	749:765	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	5	50	theme	IgG	554:556	arg1	composition					566:576	Serum IgG glycome composition	548:576	Serum IgG glycome composition	548:576	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	3	51	theme	glycosylation	405:417	arg1	profile					419:425	the immunoglobulin glycosylation profile	386:425	the immunoglobulin glycosylation profile of children with epilepsy	386:451	Here we analyzed the characteristics of the immunoglobulin glycosylation profile of children with epilepsy.
35845609	9	52	theme	inflammatory	1247:1258	arg1	reaction					1260:1267	the inflammatory reaction	1243:1267	the inflammatory reaction of the body	1243:1279	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	5	53	theme	liquid	668:673	arg1	chromatography					675:688	ultra-high-performance liquid chromatography	645:688	ultra-high-performance liquid chromatography approach	645:697	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	5	54	theme	glycome	558:564	arg1	composition					566:576	Serum IgG glycome composition	548:576	Serum IgG glycome composition	548:576	Serum IgG glycome composition was analyzed by hydrophilic interaction liquid chromatography with ultra-high-performance liquid chromatography approach.
35845609	2	55	theme	neurological	323:334	arg1	diseases					336:343	neurological diseases	323:343	neurological diseases	323:343	Studies suggested that immunoglobulin G (IgG) glycosylation are closely related to neurological diseases.
35845609	6	56	dep	%	776:776	arg1	lower					828:832	lower	828:832	lower	828:832	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	6	57	theme	epilepsy	785:792	arg1	patients					794:801	the epilepsy patients	781:801	the epilepsy patients	781:801	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	1	58	theme	chronic	124:130	arg1	disease					138:144	a chronic brain disease	122:144	a chronic brain disease that recurs during childhood	122:173	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	1	58	theme	chronic	124:130	arg1	Epilepsy					110:117	Background Epilepsy	99:117	Background Epilepsy	99:117	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	1	59	theme	epilepsy	204:211	arg1	half					190:193	more than half	180:193	more than half of adult epilepsy	180:211	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	0	60	with	Children	56:63	arg1	Epilepsy					70:77	Epilepsy	70:77	Epilepsy in Han Nationality	70:96	Expression Profile of Immunoglobulin G Glycosylation in Children With Epilepsy in Han Nationality.
35845609	1	61	theme	brain	132:136	arg1	disease					138:144	a chronic brain disease	122:144	a chronic brain disease that recurs during childhood	122:173	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	1	61	theme	brain	132:136	arg1	Epilepsy					110:117	Background Epilepsy	99:117	Background Epilepsy	99:117	Background Epilepsy is a chronic brain disease that recurs during childhood, and more than half of adult epilepsy originates from childhood.
35845609	6	62	from	%	776:776	arg1	patients					794:801	the epilepsy patients	781:801	the epilepsy patients	781:801	Results The proportion of fucosylated glycans in total IgG glycans was 93.72% in the epilepsy patients, which was significantly lower than that in the control group (94.94%).
35845609	7	63	theme	digalactosylated	921:936	arg1	glycans					938:944	total monogalactosylated and digalactosylated glycans	892:944	total monogalactosylated and digalactosylated glycans	892:944	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	7	64	theme	glycans	938:944	arg1	level					883:887	A lower level	875:887	A lower level of total monogalactosylated and digalactosylated glycans	875:944	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
35845609	9	65	theme	body	1276:1279	arg1	reaction					1260:1267	the inflammatory reaction	1243:1267	the inflammatory reaction of the body	1243:1279	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	9	66	from	occurrence	1304:1313	arg1	children					1330:1337	children	1330:1337	children	1330:1337	Conclusion The decrease of core fucosylation and galactosylation may promote the inflammatory reaction of the body and participate in the occurrence of epilepsy in children.
35845609	8	67	gly	sialylated	1146:1155	arg1	glycans					1157:1163	sialylated glycans	1146:1163	sialylated glycans	1146:1163	There was no significant difference between the two groups in bisected GlcNAc glycans and sialylated glycans.
35845609	7	68	theme	total	892:896	arg1	glycans					938:944	total monogalactosylated and digalactosylated glycans	892:944	total monogalactosylated and digalactosylated glycans	892:944	A lower level of total monogalactosylated and digalactosylated glycans were observed in the epilepsy patients group (30.76 and 40.14%) than that in the controls (36.17 and 42.69%).
37390874	12	0	theme	writhings	1841:1849	arg1	number					1821:1826	the number	1817:1826	the number of abdominal writhings	1817:1849	EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%.
37390874	4	1	theme	cell	855:858	arg1	test					870:873	carrageenan-induced cell migration test	835:873	carrageenan-induced cell migration test	835:873	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	16	2	theme	%	2319:2319	arg1	reduction					2293:2301	ear edema reduction	2283:2301	ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively	2283:2345	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	7	3	theme	oxygenated	1099:1108	arg1	classes					1086:1092	The major compounds classes	1066:1092	The major compounds classes	1066:1092	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	7	3	theme	oxygenated	1099:1108	arg1	sesquiterpenoids					1110:1125	oxygenated sesquiterpenoids	1099:1125	oxygenated sesquiterpenoids (57.3 ± 0.2%)	1099:1139	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	7	3	theme	oxygenated	1099:1108	arg1	%					1138:1138	57.3 ± 0.2%	1128:1138	57.3 ± 0.2%	1128:1138	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	3	4	theme	METHODS	512:518	arg1	EuEO					520:523	MATERIAL AND METHODS EuEO	499:523	MATERIAL AND METHODS EuEO	499:523	MATERIAL AND METHODS EuEO was obtained by hydrodistillation and analyzed by GC and GC-MS.
37390874	13	5	theme	plate	1914:1918	arg1	latency					1930:1936	increased hot plate test time latency	1900:1936	increased hot plate test time latency	1900:1936	EuEO did not show increased hot plate test time latency in any of the intervals analyzed.
37390874	4	6	theme	antinociceptive	593:607	arg1	action					609:614	The antinociceptive action	589:614	The antinociceptive action in mice	589:622	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	13	7	theme	time	1925:1928	arg1	latency					1930:1936	increased hot plate test time latency	1900:1936	increased hot plate test time latency	1900:1936	EuEO did not show increased hot plate test time latency in any of the intervals analyzed.
37390874	2	8	theme	antinociceptive	380:394	arg1	activities					419:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities	358:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO)	358:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	4	9	theme	central	661:667	arg1	activity					679:686	the peripheral and central analgesic activity	642:686	the peripheral and central analgesic activity	642:686	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	7	10	theme	major	1070:1074	arg1	classes					1086:1092	The major compounds classes	1066:1092	The major compounds classes	1066:1092	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	7	10	theme	major	1070:1074	arg1	sesquiterpenoids					1110:1125	oxygenated sesquiterpenoids	1099:1125	oxygenated sesquiterpenoids (57.3 ± 0.2%)	1099:1139	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	19	11	theme	oral	2718:2721	arg1	toxicity					2723:2730	low acute oral toxicity	2708:2730	low acute oral toxicity	2708:2730	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	10	12	theme	vehicle	1608:1614	arg1	group					1616:1620	the vehicle group	1604:1620	the vehicle group	1604:1620	EuEO (300 mg/kg) did not cause a reduction in the number of crossings in the open field compared to the vehicle group.
37390874	12	13	theme	100	1789:1791	arg1	doses					1776:1780	doses	1776:1780	doses of 50, 100, and 200 mg/kg	1776:1806	EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%.
37390874	20	14	theme	species	2802:2808	arg1	antinociceptive					2756:2770	antinociceptive	2756:2770	antinociceptive	2756:2770	This work confirms the antinociceptive and anti-inflammatory of this species as the traditional use.
37390874	8	15	theme	highest	1230:1236	arg1	concentrations					1238:1251	the highest concentrations	1226:1251	the highest concentrations	1226:1251	The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%).
37390874	19	16	theme	low	2708:2710	arg1	toxicity					2723:2730	low acute oral toxicity	2708:2730	low acute oral toxicity	2708:2730	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	16	17	theme	50	2253:2254	arg1	doses					2244:2248	doses	2244:2248	doses of 50, 100, and 200 mg/kg	2244:2274	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	5	18	theme	field	932:936	arg1	test					938:941	the open field test	923:941	the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO	923:1013	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	16	19	theme	100	2257:2259	arg1	doses					2244:2248	doses	2244:2248	doses of 50, 100, and 200 mg/kg	2244:2274	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	4	20	theme	peripheral	646:655	arg1	activity					679:686	the peripheral and central analgesic activity	642:686	the peripheral and central analgesic activity	642:686	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	8	21	theme	chemical	1199:1206	arg1	constituents					1208:1219	The chemical constituents	1195:1219	The chemical constituents with the highest concentrations	1195:1251	The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%).
37390874	8	21	theme	chemical	1199:1206	arg1	curzerene					1258:1266	curzerene	1258:1266	curzerene (33.4 ± 8.5%)	1258:1280	The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%).
37390874	4	22	theme	plate	723:727	arg1	tests					729:733	hot plate tests	719:733	hot plate tests (50, 100, and 200 mg/kg)	719:758	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	15	23	theme	paw	2093:2095	arg1	time					2105:2108	paw licking time	2093:2108	paw licking time	2093:2108	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	1	24	theme	ETHNOPHARMACOLOGICAL	140:159	arg1	RELEVANCE					161:169	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion	140:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion	140:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	4	25	theme	abdominal	694:702	arg1	contortion					704:713	abdominal contortion	694:713	abdominal contortion	694:713	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	13	26	theme	test	1920:1923	arg1	latency					1930:1936	increased hot plate test time latency	1900:1936	increased hot plate test time latency	1900:1936	EuEO did not show increased hot plate test time latency in any of the intervals analyzed.
37390874	17	27	theme	leukocyte	2373:2381	arg1	recruitment					2383:2393	leukocyte recruitment	2373:2393	leukocyte recruitment	2373:2393	Moreover, EuEO inhibited leukocyte recruitment only at a dose of 200 mg/kg.
37390874	15	28	theme	first	2152:2156	arg1	phase					2158:2162	the first phase	2148:2162	the first phase	2148:2162	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	9	29	theme	2000 mg/kg	1426:1435	arg1	doses					1404:1408	doses	1404:1408	doses of 50, 300, and 2000 mg/kg	1404:1435	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	17	30	theme	200 mg/kg	2413:2421	arg1	dose					2405:2408	a dose	2403:2408	a dose of 200 mg/kg	2403:2421	Moreover, EuEO inhibited leukocyte recruitment only at a dose of 200 mg/kg.
37390874	18	31	theme	essential	2568:2576	arg1	oil					2578:2580	essential oil	2568:2580	essential oil	2568:2580	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	9	32	dep	patterns	1466:1473	arg1	the					1453:1455	the	1453:1455	the	1453:1455	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	0	33	theme	type	88:91	arg1	oil					103:105	the curzerene type essential oil	74:105	the curzerene type essential oil of Eugenia uniflora from Brazil	74:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	18	34	theme	recruitment	2459:2469	arg1	values					2439:2444	The inhibitory values	2424:2444	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application	2424:2506	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	18	34	theme	recruitment	2459:2469	arg1	%					2517:2517	4.86%	2513:2517	4.86%	2513:2517	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	15	35	theme	%	2209:2209	arg1	inhibition					2170:2179	inhibition	2170:2179	inhibition of 30.54%, 55.02%, and 80.87%	2170:2209	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	11	36	theme	aspartate	1627:1635	arg1	aminotransferase					1637:1652	aspartate aminotransferase	1627:1652	The aspartate aminotransferase (AST) level	1623:1664	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	11	36	theme	aspartate	1627:1635	arg1	AST					1655:1657	AST	1655:1657	AST	1655:1657	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	0	37	theme	oil	103:105	arg1	activities					60:69	antinociceptive and anti-inflammatory activities	22:69	antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil	22:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	0	37	theme	oil	103:105	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	15	38	theme	100	2126:2128	arg1	doses					2113:2117	doses	2113:2117	doses of 50, 100, and 200 mg/kg	2113:2143	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	19	39	dep	CONCLUSION	2597:2606	arg1	has					2639:2641	has	2639:2641	has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity	2639:2730	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	0	40	from	activities	60:69	arg1	Brazil					132:137	Brazil	132:137	Brazil	132:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	0	41	theme	Eugenia	110:116	arg1	uniflora					118:125	Eugenia uniflora	110:125	Eugenia uniflora	110:125	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	11	42	dep	groups	1693:1698	arg1	2000 mg/kg					1708:1717	2000 mg/kg	1708:1717	2000 mg/kg	1708:1717	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	11	42	dep	groups	1693:1698	arg1	50					1701:1702	50	1701:1702	50	1701:1702	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	15	43	theme	%	2197:2197	arg1	inhibition					2170:2179	inhibition	2170:2179	inhibition of 30.54%, 55.02%, and 80.87%	2170:2209	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	9	44	theme	behavior	1457:1464	arg1	patterns					1466:1473	behavior patterns	1457:1473	behavior patterns	1457:1473	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	2	45	theme	Eugenia	460:466	arg1	EuEO					492:495	EuEO	492:495	EuEO	492:495	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	2	45	theme	Eugenia	460:466	arg1	oil					487:489	Eugenia uniflora essential oil	460:489	Eugenia uniflora essential oil (EuEO)	460:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	1	46	dep	Eugenia	175:181	arg1	uniflora					183:190	uniflora	183:190	uniflora	183:190	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	11	47	theme	control	1741:1747	arg1	p < 0.05					1756:1763	p < 0.05	1756:1763	p < 0.05	1756:1763	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	11	47	theme	control	1741:1747	arg1	group					1749:1753	the control group	1737:1753	the control group (p < 0.05)	1737:1764	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	0	48	theme	Chemical	0:7	arg1	activities					60:69	antinociceptive and anti-inflammatory activities	22:69	antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil	22:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	0	48	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	1	49	theme	inflammatory	285:296	arg1	diseases					311:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	4	50	dep	tests	729:733	arg1	100					740:742	100	740:742	100	740:742	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	4	50	dep	tests	729:733	arg1	200 mg/kg					749:757	200 mg/kg	749:757	200 mg/kg	749:757	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	4	50	dep	tests	729:733	arg1	50					736:737	50	736:737	50	736:737	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	2	51	theme	chemotype	447:455	arg1	activities					419:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities	358:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO)	358:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	1	52	dep	RELEVANCE	161:169	arg1	infusion					197:204	The Eugenia uniflora leaf infusion	171:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion	140:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	14	53	theme	licking	2005:2011	arg1	time					2013:2016	paw licking time	2001:2016	paw licking time	2001:2016	At 200 mg/kg, EuEO decreased paw licking time, with inhibition of 63.43%.
37390874	1	54	theme	diuretic	302:309	arg1	diseases					311:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	13	55	theme	hot	1910:1912	arg1	latency					1930:1936	increased hot plate test time latency	1900:1936	increased hot plate test time latency	1900:1936	EuEO did not show increased hot plate test time latency in any of the intervals analyzed.
37390874	9	56	theme	Oral	1375:1378	arg1	treatment					1380:1388	Oral treatment	1375:1388	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg,	1375:1436	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	9	57	theme	animals	1495:1501	arg1	mortality					1478:1486	mortality	1478:1486	mortality	1478:1486	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	9	57	theme	animals	1495:1501	arg1	patterns					1466:1473	behavior patterns	1457:1473	behavior patterns	1457:1473	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	2	58	theme	essential	477:485	arg1	EuEO					492:495	EuEO	492:495	EuEO	492:495	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	2	58	theme	essential	477:485	arg1	oil					487:489	Eugenia uniflora essential oil	460:489	Eugenia uniflora essential oil (EuEO)	460:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	10	59	theme	open	1581:1584	arg1	field					1586:1590	the open field	1577:1590	the open field	1577:1590	EuEO (300 mg/kg) did not cause a reduction in the number of crossings in the open field compared to the vehicle group.
37390874	10	60	from	reduction	1537:1545	arg1	number					1554:1559	the number	1550:1559	the number of crossings	1550:1572	EuEO (300 mg/kg) did not cause a reduction in the number of crossings in the open field compared to the vehicle group.
37390874	16	61	theme	edema	2287:2291	arg1	reduction					2293:2301	ear edema reduction	2283:2301	ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively	2283:2345	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	4	62	theme	ear	776:778	arg1	swelling					780:787	xylene-induced ear swelling	761:787	xylene-induced ear swelling	761:787	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	12	63	from	doses	1776:1780	arg1	EuEO					1767:1770	EuEO	1767:1770	EuEO	1767:1770	EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%.
37390874	5	64	theme	Spontaneous	876:886	arg1	activity					898:905	Spontaneous locomotor activity	876:905	Spontaneous locomotor activity	876:905	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	2	65	theme	toxic	373:377	arg1	activities					419:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities	358:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO)	358:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	1	66	theme	gastroenteritis	247:261	arg1	diseases					311:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	20	67	theme	traditional	2817:2827	arg1	use					2829:2831	the traditional use	2813:2831	the traditional use	2813:2831	This work confirms the antinociceptive and anti-inflammatory of this species as the traditional use.
37390874	5	68	theme	relaxant	990:997	arg1	effects					999:1005	any nonspecific sedative or muscle relaxant effects	955:1005	any nonspecific sedative or muscle relaxant effects of EuEO	955:1013	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	16	69	theme	%	2311:2311	arg1	reduction					2293:2301	ear edema reduction	2283:2301	ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively	2283:2345	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	1	70	theme	fever	264:268	arg1	diseases					311:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	12	71	theme	abdominal	1831:1839	arg1	writhings					1841:1849	abdominal writhings	1831:1849	abdominal writhings	1831:1849	EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%.
37390874	4	72	theme	carrageenan-induced	835:853	arg1	test					870:873	carrageenan-induced cell migration test	835:873	carrageenan-induced cell migration test	835:873	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	6	73	theme	%	1063:1063	arg1	yield					1045:1049	a yield	1043:1049	a yield of 2.6 ± 0.7%	1043:1063	RESULTS The EuEO displayed a yield of 2.6 ± 0.7%.
37390874	5	74	theme	nonspecific	959:969	arg1	effects					999:1005	any nonspecific sedative or muscle relaxant effects	955:1005	any nonspecific sedative or muscle relaxant effects of EuEO	955:1013	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	8	75	with	constituents	1208:1219	arg1	concentrations					1238:1251	the highest concentrations	1226:1251	the highest concentrations	1226:1251	The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%).
37390874	7	76	theme	compounds	1076:1084	arg1	classes					1086:1092	The major compounds classes	1066:1092	The major compounds classes	1066:1092	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	7	76	theme	compounds	1076:1084	arg1	sesquiterpenoids					1110:1125	oxygenated sesquiterpenoids	1099:1125	oxygenated sesquiterpenoids (57.3 ± 0.2%)	1099:1139	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	1	77	theme	hypertension	271:282	arg1	diseases					311:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	gastroenteritis, fever, hypertension, inflammatory and diuretic diseases	247:318	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	12	78	theme	200 mg/kg	1798:1806	arg1	doses					1776:1780	doses	1776:1780	doses of 50, 100, and 200 mg/kg	1776:1806	EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%.
37390874	4	79	theme	migration	860:868	arg1	test					870:873	carrageenan-induced cell migration test	835:873	carrageenan-induced cell migration test	835:873	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	5	80	theme	locomotor	888:896	arg1	activity					898:905	Spontaneous locomotor activity	876:905	Spontaneous locomotor activity	876:905	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	19	81	theme	anti-inflammatory	2675:2691	arg1	activities					2693:2702	significant antinociceptive and anti-inflammatory activities	2643:2702	significant antinociceptive and anti-inflammatory activities	2643:2702	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	1	82	theme	leaf	192:195	arg1	infusion					197:204	The Eugenia uniflora leaf infusion	171:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion	140:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	4	83	theme	nociception	813:823	arg1	test					825:828	the nociception test	809:828	the nociception test	809:828	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	4	84	from	action	609:614	arg1	mice					619:622	mice	619:622	mice	619:622	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	19	85	contain	has	2639:2641	arg2	toxicity					2723:2730	low acute oral toxicity	2708:2730	low acute oral toxicity	2708:2730	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	19	85	contain	has	2639:2641	arg1	EuEO					2612:2615	The EuEO	2608:2615	The EuEO	2608:2615	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	19	85	contain	has	2639:2641	arg1	chemotype					2628:2636	curzerene chemotype	2618:2636	curzerene chemotype	2618:2636	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	19	85	contain	has	2639:2641	arg2	activities					2693:2702	significant antinociceptive and anti-inflammatory activities	2643:2702	significant antinociceptive and anti-inflammatory activities	2643:2702	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	4	86	theme	analgesic	669:677	arg1	activity					679:686	the peripheral and central analgesic activity	642:686	the peripheral and central analgesic activity	642:686	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	19	87	theme	acute	2712:2716	arg1	toxicity					2723:2730	low acute oral toxicity	2708:2730	low acute oral toxicity	2708:2730	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	19	88	dep	significant	2643:2653	arg1	antinociceptive					2655:2669	antinociceptive	2655:2669	antinociceptive	2655:2669	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	1	89	theme	folk	224:227	arg1	medicine					229:236	folk medicine	224:236	folk medicine	224:236	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	16	90	theme	200 mg/kg	2266:2274	arg1	doses					2244:2248	doses	2244:2248	doses of 50, 100, and 200 mg/kg	2244:2274	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	18	91	theme	carrageenan	2484:2494	arg1	application					2496:2506	carrageenan application	2484:2506	carrageenan application	2484:2506	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	13	92	theme	increased	1900:1908	arg1	latency					1930:1936	increased hot plate test time latency	1900:1936	increased hot plate test time latency	1900:1936	EuEO did not show increased hot plate test time latency in any of the intervals analyzed.
37390874	15	93	theme	%	2189:2189	arg1	inhibition					2170:2179	inhibition	2170:2179	inhibition of 30.54%, 55.02%, and 80.87%	2170:2209	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	9	94	theme	50	1413:1414	arg1	doses					1404:1408	doses	1404:1408	doses of 50, 300, and 2000 mg/kg	1404:1435	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	18	95	theme	leukocyte	2449:2457	arg1	recruitment					2459:2469	leukocyte recruitment	2449:2469	leukocyte recruitment	2449:2469	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	16	96	theme	ear	2283:2285	arg1	edema					2287:2291	ear edema	2283:2291	ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively	2283:2345	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	1	97	used	used	216:219	arg2	RELEVANCE					161:169	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion	140:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion	140:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	4	98	theme	hot	719:721	arg1	tests					729:733	hot plate tests	719:733	hot plate tests (50, 100, and 200 mg/kg)	719:758	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	11	99	theme	EuEO-treated	1680:1691	arg1	groups					1693:1698	EuEO-treated groups	1680:1698	EuEO-treated groups (50 and 2000 mg/kg)	1680:1718	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	15	100	theme	licking	2097:2103	arg1	time					2105:2108	paw licking time	2093:2108	paw licking time	2093:2108	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	9	101	theme	300	1417:1419	arg1	doses					1404:1408	doses	1404:1408	doses of 50, 300, and 2000 mg/kg	1404:1435	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	0	102	theme	curzerene	78:86	arg1	oil					103:105	the curzerene type essential oil	74:105	the curzerene type essential oil of Eugenia uniflora from Brazil	74:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	20	103	dep	antinociceptive	2756:2770	arg1	the					2752:2754	the	2752:2754	the	2752:2754	This work confirms the antinociceptive and anti-inflammatory of this species as the traditional use.
37390874	8	104	theme	caryophyllene	1283:1295	arg1	oxide					1297:1301	caryophyllene oxide	1283:1301	caryophyllene oxide (7.6 ± 2.8%)	1283:1314	The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%).
37390874	8	104	theme	caryophyllene	1283:1295	arg1	%					1313:1313	7.6 ± 2.8%	1304:1313	7.6 ± 2.8%	1304:1313	The chemical constituents with the highest concentrations were curzerene (33.4 ± 8.5%), caryophyllene oxide (7.6 ± 2.8%), β-elemene (6.5 ± 1.8%), and E-caryophyllene (4.1 ± 0.3%).
37390874	0	105	theme	essential	93:101	arg1	oil					103:105	the curzerene type essential oil	74:105	the curzerene type essential oil of Eugenia uniflora from Brazil	74:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	1	106	theme	Eugenia	175:181	arg1	infusion					197:204	The Eugenia uniflora leaf infusion	171:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion	140:204	ETHNOPHARMACOLOGICAL RELEVANCE The Eugenia uniflora leaf infusion is widely used in folk medicine to treat gastroenteritis, fever, hypertension, inflammatory and diuretic diseases.
37390874	14	107	theme	%	2043:2043	arg1	inhibition					2024:2033	inhibition	2024:2033	inhibition of 63.43%	2024:2043	At 200 mg/kg, EuEO decreased paw licking time, with inhibition of 63.43%.
37390874	15	108	theme	200 mg/kg	2135:2143	arg1	doses					2113:2117	doses	2113:2117	doses of 50, 100, and 200 mg/kg	2113:2143	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	6	109	dep	RESULTS	1016:1022	arg1	EuEO					1028:1031	EuEO	1028:1031	EuEO	1028:1031	RESULTS The EuEO displayed a yield of 2.6 ± 0.7%.
37390874	10	110	theme	crossings	1564:1572	arg1	number					1554:1559	the number	1550:1559	the number of crossings	1550:1572	EuEO (300 mg/kg) did not cause a reduction in the number of crossings in the open field compared to the vehicle group.
37390874	0	111	theme	uniflora	118:125	arg1	oil					103:105	the curzerene type essential oil	74:105	the curzerene type essential oil of Eugenia uniflora from Brazil	74:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	18	112	theme	application	2496:2506	arg1	4 h					2477:2479	4 h	2477:2479	4 h of carrageenan application	2477:2506	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	11	113	theme	aminotransferase	1637:1652	arg1	higher					1670:1675	higher	1670:1675	higher	1670:1675	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	11	113	theme	aminotransferase	1637:1652	arg1	level					1660:1664	The aspartate aminotransferase (AST) level	1623:1664	The aspartate aminotransferase (AST) level	1623:1664	The aspartate aminotransferase (AST) level was higher in EuEO-treated groups (50 and 2000 mg/kg) when compared to the control group (p < 0.05).
37390874	7	114	theme	sesquiterpene	1154:1166	arg1	16.4 ± 2.6					1182:1191	16.4 ± 2.6	1182:1191	16.4 ± 2.6	1182:1191	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	7	114	theme	sesquiterpene	1154:1166	arg1	hydrocarbons					1168:1179	sesquiterpene hydrocarbons	1154:1179	sesquiterpene hydrocarbons (16.4 ± 2.6)	1154:1192	The major compounds classes were oxygenated sesquiterpenoids (57.3 ± 0.2%), followed by sesquiterpene hydrocarbons (16.4 ± 2.6).
37390874	5	115	theme	open	927:930	arg1	test					938:941	the open field test	923:941	the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO	923:1013	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	16	116	theme	%	2331:2331	arg1	reduction					2293:2301	ear edema reduction	2283:2301	ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively	2283:2345	The groups treated with EuEO at doses of 50, 100, and 200 mg/kg showed ear edema reduction of 50.26%, 55.17%, and 51.31%, respectively.
37390874	2	117	dep	STUDY	332:336	arg1	work					343:346	This work	338:346	THE STUDY This work	328:346	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	0	118	theme	antinociceptive	22:36	arg1	activities					60:69	antinociceptive and anti-inflammatory activities	22:69	antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil	22:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	0	118	theme	antinociceptive	22:36	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	18	119	theme	oil	2578:2580	arg1	50					2542:2543	50	2542:2543	50	2542:2543	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	18	119	theme	oil	2578:2580	arg1	oil					2578:2580	essential oil	2568:2580	essential oil	2568:2580	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	2	120	theme	curzerene	437:445	arg1	chemotype					447:455	the curzerene chemotype	433:455	the curzerene chemotype of Eugenia uniflora essential oil (EuEO)	433:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	14	121	theme	paw	2001:2003	arg1	time					2013:2016	paw licking time	2001:2016	paw licking time	2001:2016	At 200 mg/kg, EuEO decreased paw licking time, with inhibition of 63.43%.
37390874	0	122	theme	anti-inflammatory	42:58	arg1	activities					60:69	antinociceptive and anti-inflammatory activities	22:69	antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil	22:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	0	122	theme	anti-inflammatory	42:58	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	2	123	theme	oil	487:489	arg1	chemotype					447:455	the curzerene chemotype	433:455	the curzerene chemotype of Eugenia uniflora essential oil (EuEO)	433:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	15	124	theme	formalin-induced	2049:2064	arg1	pain					2072:2075	formalin-induced acute pain	2049:2075	formalin-induced acute pain	2049:2075	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	19	125	theme	curzerene	2618:2626	arg1	EuEO					2612:2615	The EuEO	2608:2615	The EuEO	2608:2615	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	19	125	theme	curzerene	2618:2626	arg1	chemotype					2628:2636	curzerene chemotype	2618:2636	curzerene chemotype	2618:2636	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	2	126	theme	anti-inflammatory	401:417	arg1	activities					419:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities	358:428	the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO)	358:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	5	127	theme	EuEO	1010:1013	arg1	effects					999:1005	any nonspecific sedative or muscle relaxant effects	955:1005	any nonspecific sedative or muscle relaxant effects of EuEO	955:1013	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	15	128	theme	50	2122:2123	arg1	doses					2113:2117	doses	2113:2117	doses of 50, 100, and 200 mg/kg	2113:2143	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
37390874	2	129	theme	uniflora	468:475	arg1	EuEO					492:495	EuEO	492:495	EuEO	492:495	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	2	129	theme	uniflora	468:475	arg1	oil					487:489	Eugenia uniflora essential oil	460:489	Eugenia uniflora essential oil (EuEO)	460:496	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	3	130	theme	MATERIAL	499:506	arg1	EuEO					520:523	MATERIAL AND METHODS EuEO	499:523	MATERIAL AND METHODS EuEO	499:523	MATERIAL AND METHODS EuEO was obtained by hydrodistillation and analyzed by GC and GC-MS.
37390874	5	131	theme	sedative	971:978	arg1	effects					999:1005	any nonspecific sedative or muscle relaxant effects	955:1005	any nonspecific sedative or muscle relaxant effects of EuEO	955:1013	Spontaneous locomotor activity was assessed in the open field test to rule out any nonspecific sedative or muscle relaxant effects of EuEO.
37390874	9	132	with	treatment	1380:1388	arg1	EuEO					1395:1398	EuEO	1395:1398	EuEO	1395:1398	Oral treatment with EuEO, at doses of 50, 300, and 2000 mg/kg, did not change the behavior patterns or mortality of the animals.
37390874	4	133	theme	xylene-induced	761:774	arg1	swelling					780:787	xylene-induced ear swelling	761:787	xylene-induced ear swelling	761:787	The antinociceptive action in mice was evaluated for the peripheral and central analgesic activity using abdominal contortion and hot plate tests (50, 100, and 200 mg/kg); xylene-induced ear swelling was carried out for the nociception test, and carrageenan-induced cell migration test.
37390874	19	134	theme	significant	2643:2653	arg1	activities					2693:2702	significant antinociceptive and anti-inflammatory activities	2643:2702	significant antinociceptive and anti-inflammatory activities	2643:2702	CONCLUSION The EuEO, curzerene chemotype, has significant antinociceptive and anti-inflammatory activities and low acute oral toxicity.
37390874	2	135	theme	STUDY	332:336	arg1	AIM					321:323	AIM	321:323	AIM OF THE STUDY This work	321:346	AIM OF THE STUDY This work evaluated the acute oral toxic, antinociceptive, and anti-inflammatory activities of the curzerene chemotype of Eugenia uniflora essential oil (EuEO).
37390874	0	136	from	Brazil	132:137	arg1	activities					60:69	antinociceptive and anti-inflammatory activities	22:69	antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil	22:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	0	136	from	Brazil	132:137	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	0	136	from	Brazil	132:137	arg1	oil					103:105	the curzerene type essential oil	74:105	the curzerene type essential oil of Eugenia uniflora from Brazil	74:137	Chemical composition, antinociceptive and anti-inflammatory activities of the curzerene type essential oil of Eugenia uniflora from Brazil.
37390874	12	137	theme	50	1785:1786	arg1	doses					1776:1780	doses	1776:1780	doses of 50, 100, and 200 mg/kg	1776:1806	EuEO, at doses of 50, 100, and 200 mg/kg, reduced the number of abdominal writhings by 61.66%, 38.33%, and 33.33%.
37390874	18	138	theme	inhibitory	2428:2437	arg1	values					2439:2444	The inhibitory values	2424:2444	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application	2424:2506	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	18	138	theme	inhibitory	2428:2437	arg1	%					2517:2517	4.86%	2513:2517	4.86%	2513:2517	The inhibitory values of leukocyte recruitment after 4 h of carrageenan application were 4.86%, 4.93%, and 47.25% for 50, 100, and 200 mg/kg of essential oil, respectively.
37390874	15	139	theme	acute	2066:2070	arg1	pain					2072:2075	formalin-induced acute pain	2049:2075	formalin-induced acute pain	2049:2075	In formalin-induced acute pain, EuEO decreased paw licking time at doses of 50, 100, and 200 mg/kg in the first phase, with inhibition of 30.54%, 55.02%, and 80.87%.
36414090	7	0	theme	DNA	1157:1159	arg1	fragmentation					1162:1174	deoxyribonucleic acid (DNA) fragmentation	1134:1174	deoxyribonucleic acid (DNA) fragmentation	1134:1174	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	9	1	theme	kappa	1682:1686	arg1	IκB-α					1697:1701	IκB-α	1697:1701	IκB-α	1697:1701	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	1	theme	kappa	1682:1686	arg1	alpha					1690:1694	nuclear kappa B alpha	1674:1694	nuclear kappa B alpha (IκB-α)	1674:1702	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	7	2	theme	acid	1151:1154	arg1	fragmentation					1162:1174	deoxyribonucleic acid (DNA) fragmentation	1134:1174	deoxyribonucleic acid (DNA) fragmentation	1134:1174	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	8	3	theme	histopathological	1220:1236	arg1	changes					1238:1244	post-incisional histopathological changes	1204:1244	post-incisional histopathological changes	1204:1244	Magnolol markedly reverses post-incisional histopathological changes and biochemical composition of the incised paw.
36414090	9	4	theme	alpha	1690:1694	arg1	inhibitor					1661:1669	inhibitor	1661:1669	inhibitor of nuclear kappa B alpha (IκB-α)	1661:1702	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	2	5	theme	antioxidant	294:304	arg1	potential					326:334	the anti-inflammatory, antioxidant, and cytoprotective potential	271:334	the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol	271:346	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	9	6	theme	factor	1493:1498	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	6	theme	factor	1493:1498	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	2	7	theme	magnolol	339:346	arg1	potential					326:334	the anti-inflammatory, antioxidant, and cytoprotective potential	271:334	the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol	271:346	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	9	8	theme	light	1506:1510	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	8	theme	light	1506:1510	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	4	9	theme	incision	634:641	arg1	surgery					643:649	plantar incision surgery	626:649	plantar incision surgery	626:649	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	10	10	theme	inflammatory	1809:1820	arg1	pain					1834:1837	post-incisional inflammatory nociceptive pain	1793:1837	post-incisional inflammatory nociceptive pain	1793:1837	SIGNIFICANCE The present study strongly suggests that magnolol significantly suppressed post-incisional inflammatory nociceptive pain by targeting TRPV1/P2Y and TLR4/NF-κB signaling.
36414090	1	11	theme	inflammatory	214:225	arg1	pain					239:242	post-incisional inflammatory nociceptive pain	198:242	post-incisional inflammatory nociceptive pain	198:242	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	6	12	theme	neutrophil	972:981	arg1	infiltration					983:994	neutrophil infiltration	972:994	neutrophil infiltration	972:994	Magnolol promisingly inhibited post-incision induces nitric oxide (NO), malondialdehyde (MDA), eosinophil peroxidase (EPO), and neutrophil infiltration.
36414090	0	13	from	signaling	80:88	arg1	model					114:118	a postoperative pain model	93:118	a postoperative pain model	93:118	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	3	14	theme	planter	442:448	arg1	surgery					459:465	planter incision surgery	442:465	planter incision surgery	442:465	Next, an in-vivo model of planter incision surgery was established in BALB/c mice.
36414090	9	15	theme	activated	1530:1538	arg1	cell					1542:1545	activated B cell	1530:1545	activated B cell	1530:1545	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	8	16	theme	paw	1289:1291	arg1	changes					1238:1244	post-incisional histopathological changes	1204:1244	post-incisional histopathological changes	1204:1244	Magnolol markedly reverses post-incisional histopathological changes and biochemical composition of the incised paw.
36414090	8	16	theme	paw	1289:1291	arg1	composition					1262:1272	biochemical composition	1250:1272	biochemical composition of the incised paw	1250:1291	Magnolol markedly reverses post-incisional histopathological changes and biochemical composition of the incised paw.
36414090	5	17	theme	KEY	690:692	arg1	FINDINGS					694:701	KEY FINDINGS	690:701	KEY FINDINGS	690:701	KEY FINDINGS The results indicate that magnolol significantly suppressed post-incision-induced mechanical allodynia, thermal hyperalgesia, and paw edema.
36414090	9	18	theme	post-incisional	1327:1341	arg1	expression					1352:1361	post-incisional increase expression	1327:1361	post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4)	1327:1482	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	19	theme	cell	1542:1545	arg1	cyclooxygenase-2					1556:1571	cyclooxygenase-2	1556:1571	cyclooxygenase-2 (COX-2)	1556:1579	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	19	theme	cell	1542:1545	arg1	synthase					1608:1615	inducible nitric oxide synthase	1585:1615	inducible nitric oxide synthase (iNOS)	1585:1622	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	19	theme	cell	1542:1545	arg1	COX-2					1574:1578	COX-2	1574:1578	COX-2	1574:1578	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	19	theme	cell	1542:1545	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	19	theme	cell	1542:1545	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	19	theme	cell	1542:1545	arg1	iNOS					1618:1621	iNOS	1618:1621	iNOS	1618:1621	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	19	theme	cell	1542:1545	arg1	expression					1352:1361	post-incisional increase expression	1327:1361	post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4)	1327:1482	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	7	20	theme	factor-α	1079:1086	arg1	release					1053:1059	the release	1049:1059	the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β)	1049:1118	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	2	21	theme	anti-inflammatory	275:291	arg1	potential					326:334	the anti-inflammatory, antioxidant, and cytoprotective potential	271:334	the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol	271:346	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	1	22	theme	current	130:136	arg1	study					138:142	The current study	126:142	The current study	126:142	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	9	23	theme	transient	1366:1374	arg1	vanilloid					1395:1403	transient receptor potential vanilloid 1	1366:1405	transient receptor potential vanilloid 1 (TRPV1)	1366:1413	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	23	theme	transient	1366:1374	arg1	TRPV1					1408:1412	TRPV1	1408:1412	TRPV1	1408:1412	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	1	24	from	activity	174:181	arg1	pain					239:242	post-incisional inflammatory nociceptive pain	198:242	post-incisional inflammatory nociceptive pain	198:242	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	4	25	theme	intraperitoneal	517:531	arg1	50 mg/kg					508:515	Tramadol 50 mg/kg intraperitoneal	499:531	Tramadol 50 mg/kg intraperitoneal (i.p.)	499:538	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	9	26	theme	potential	1385:1393	arg1	vanilloid					1395:1403	transient receptor potential vanilloid 1	1366:1405	transient receptor potential vanilloid 1 (TRPV1)	1366:1413	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	26	theme	potential	1385:1393	arg1	TRPV1					1408:1412	TRPV1	1408:1412	TRPV1	1408:1412	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	1	27	theme	anti-nociceptive	157:172	arg1	activity					174:181	the anti-nociceptive activity	153:181	the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain	153:242	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	2	28	dep	-induced	395:402	arg1	peroxide					380:387	hydrogen peroxide	371:387	hydrogen peroxide	371:387	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	3	29	theme	incision	450:457	arg1	surgery					459:465	planter incision surgery	442:465	planter incision surgery	442:465	Next, an in-vivo model of planter incision surgery was established in BALB/c mice.
36414090	0	30	theme	pain	109:112	arg1	model					114:118	a postoperative pain model	93:118	a postoperative pain model	93:118	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	9	31	theme	inducible	1585:1593	arg1	iNOS					1618:1621	iNOS	1618:1621	iNOS	1618:1621	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	31	theme	inducible	1585:1593	arg1	synthase					1608:1615	inducible nitric oxide synthase	1585:1615	inducible nitric oxide synthase (iNOS)	1585:1622	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	4	32	theme	Tramadol	499:506	arg1	50 mg/kg					508:515	Tramadol 50 mg/kg intraperitoneal	499:531	Tramadol 50 mg/kg intraperitoneal (i.p.)	499:538	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	10	33	dep	SIGNIFICANCE	1705:1716	arg1	suggests					1745:1752	suggests	1745:1752	suggests that magnolol significantly suppressed post-incisional inflammatory nociceptive pain by targeting TRPV1/P2Y and TLR4/NF-κB signaling	1745:1885	SIGNIFICANCE The present study strongly suggests that magnolol significantly suppressed post-incisional inflammatory nociceptive pain by targeting TRPV1/P2Y and TLR4/NF-κB signaling.
36414090	0	34	theme	Anti-nociceptive	0:15	arg1	effects					17:23	Anti-nociceptive effects	0:23	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.	0:119	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	4	35	dep	50 mg/kg	508:515	arg1	i.p.					534:537	i.p.	534:537	i.p.	534:537	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	9	36	theme	oxide	1602:1606	arg1	iNOS					1618:1621	iNOS	1618:1621	iNOS	1618:1621	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	36	theme	oxide	1602:1606	arg1	synthase					1608:1615	inducible nitric oxide synthase	1585:1615	inducible nitric oxide synthase (iNOS)	1585:1622	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	3	37	theme	in-vivo	425:431	arg1	model					433:437	an in-vivo model	422:437	an in-vivo model of planter incision surgery	422:465	Next, an in-vivo model of planter incision surgery was established in BALB/c mice.
36414090	10	38	theme	present	1722:1728	arg1	study					1730:1734	The present study	1718:1734	The present study	1718:1734	SIGNIFICANCE The present study strongly suggests that magnolol significantly suppressed post-incisional inflammatory nociceptive pain by targeting TRPV1/P2Y and TLR4/NF-κB signaling.
36414090	2	39	theme	-induced	395:402	arg1	cells					409:413	hydrogen peroxide (H2O2)-induced PC12 cells	371:413	hydrogen peroxide (H2O2)-induced PC12 cells	371:413	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	9	40	theme	receptor	1466:1473	arg1	cyclooxygenase-2					1556:1571	cyclooxygenase-2	1556:1571	cyclooxygenase-2 (COX-2)	1556:1579	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	40	theme	receptor	1466:1473	arg1	synthase					1608:1615	inducible nitric oxide synthase	1585:1615	inducible nitric oxide synthase (iNOS)	1585:1622	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	40	theme	receptor	1466:1473	arg1	COX-2					1574:1578	COX-2	1574:1578	COX-2	1574:1578	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	40	theme	receptor	1466:1473	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	40	theme	receptor	1466:1473	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	40	theme	receptor	1466:1473	arg1	iNOS					1618:1621	iNOS	1618:1621	iNOS	1618:1621	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	40	theme	receptor	1466:1473	arg1	expression					1352:1361	post-incisional increase expression	1327:1361	post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4)	1327:1482	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	5	41	theme	paw	833:835	arg1	edema					837:841	paw edema	833:841	paw edema	833:841	KEY FINDINGS The results indicate that magnolol significantly suppressed post-incision-induced mechanical allodynia, thermal hyperalgesia, and paw edema.
36414090	9	42	theme	inhibitor	1661:1669	arg1	expression					1647:1656	the expression	1643:1656	the expression of inhibitor of nuclear kappa B alpha (IκB-α)	1643:1702	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	6	43	theme	eosinophil	939:948	arg1	EPO					962:964	EPO	962:964	EPO	962:964	Magnolol promisingly inhibited post-incision induces nitric oxide (NO), malondialdehyde (MDA), eosinophil peroxidase (EPO), and neutrophil infiltration.
36414090	6	43	theme	eosinophil	939:948	arg1	peroxidase					950:959	eosinophil peroxidase	939:959	eosinophil peroxidase (EPO)	939:965	Magnolol promisingly inhibited post-incision induces nitric oxide (NO), malondialdehyde (MDA), eosinophil peroxidase (EPO), and neutrophil infiltration.
36414090	9	44	theme	nuclear	1674:1680	arg1	IκB-α					1697:1701	IκB-α	1697:1701	IκB-α	1697:1701	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	44	theme	nuclear	1674:1680	arg1	alpha					1690:1694	nuclear kappa B alpha	1674:1694	nuclear kappa B alpha (IκB-α)	1674:1702	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	7	45	theme	deoxyribonucleic	1134:1149	arg1	fragmentation					1162:1174	deoxyribonucleic acid (DNA) fragmentation	1134:1174	deoxyribonucleic acid (DNA) fragmentation	1134:1174	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	2	46	theme	cytoprotective	311:324	arg1	potential					326:334	the anti-inflammatory, antioxidant, and cytoprotective potential	271:334	the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol	271:346	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	9	47	theme	B	1688:1688	arg1	IκB-α					1697:1701	IκB-α	1697:1701	IκB-α	1697:1701	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	47	theme	B	1688:1688	arg1	alpha					1690:1694	nuclear kappa B alpha	1674:1694	nuclear kappa B alpha (IκB-α)	1674:1702	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	4	48	theme	intra	587:591	arg1	planter					593:599	0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter	554:599	0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter	554:599	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	4	48	theme	intra	587:591	arg1	magnolol					544:551	magnolol	544:551	magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter)	544:600	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	1	49	theme	magnolol	186:193	arg1	activity					174:181	the anti-nociceptive activity	153:181	the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain	153:242	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	9	50	theme	nuclear	1485:1491	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	50	theme	nuclear	1485:1491	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	8	51	theme	post-incisional	1204:1218	arg1	changes					1238:1244	post-incisional histopathological changes	1204:1244	post-incisional histopathological changes	1204:1244	Magnolol markedly reverses post-incisional histopathological changes and biochemical composition of the incised paw.
36414090	4	52	theme	behavior	655:662	arg1	parameters					664:673	behavior parameters	655:673	behavior parameters	655:673	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	10	53	theme	post-incisional	1793:1807	arg1	pain					1834:1837	post-incisional inflammatory nociceptive pain	1793:1837	post-incisional inflammatory nociceptive pain	1793:1837	SIGNIFICANCE The present study strongly suggests that magnolol significantly suppressed post-incisional inflammatory nociceptive pain by targeting TRPV1/P2Y and TLR4/NF-κB signaling.
36414090	9	54	theme	kappa	1500:1504	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	54	theme	kappa	1500:1504	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	10	55	theme	nociceptive	1822:1832	arg1	pain					1834:1837	post-incisional inflammatory nociceptive pain	1793:1837	post-incisional inflammatory nociceptive pain	1793:1837	SIGNIFICANCE The present study strongly suggests that magnolol significantly suppressed post-incisional inflammatory nociceptive pain by targeting TRPV1/P2Y and TLR4/NF-κB signaling.
36414090	9	56	theme	chain	1512:1516	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	56	theme	chain	1512:1516	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	8	57	theme	biochemical	1250:1260	arg1	composition					1262:1272	biochemical composition	1250:1272	biochemical composition of the incised paw	1250:1291	Magnolol markedly reverses post-incisional histopathological changes and biochemical composition of the incised paw.
36414090	4	58	theme	plantar	626:632	arg1	surgery					643:649	plantar incision surgery	626:649	plantar incision surgery	626:649	Tramadol 50 mg/kg intraperitoneal (i.p.) and magnolol (0.1, 1, 10 mg/kg i.p. + 10 mg/kg intra planter) were administered after plantar incision surgery and behavior parameters were measured.
36414090	10	59	theme	TLR4/NF-κB	1866:1875	arg1	signaling					1877:1885	TLR4/NF-κB signaling	1866:1885	TLR4/NF-κB signaling	1866:1885	SIGNIFICANCE The present study strongly suggests that magnolol significantly suppressed post-incisional inflammatory nociceptive pain by targeting TRPV1/P2Y and TLR4/NF-κB signaling.
36414090	1	60	theme	nociceptive	227:237	arg1	pain					239:242	post-incisional inflammatory nociceptive pain	198:242	post-incisional inflammatory nociceptive pain	198:242	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	2	61	theme	MAIN	245:248	arg1	METHODS					250:256	MAIN METHODS	245:256	MAIN METHODS	245:256	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	7	62	theme	tumor	1064:1068	arg1	TNF-α					1089:1093	TNF-α	1089:1093	TNF-α	1089:1093	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	7	62	theme	tumor	1064:1068	arg1	interleukin-1β					1097:1110	interleukin-1β	1097:1110	interleukin-1β (IL-1β)	1097:1118	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	7	62	theme	tumor	1064:1068	arg1	factor-α					1079:1086	tumor necrosis factor-α	1064:1086	tumor necrosis factor-α (TNF-α)	1064:1094	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	9	63	theme	B	1540:1540	arg1	cell					1542:1545	activated B cell	1530:1545	activated B cell	1530:1545	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	8	64	theme	incised	1281:1287	arg1	paw					1289:1291	the incised paw	1277:1291	the incised paw	1277:1291	Magnolol markedly reverses post-incisional histopathological changes and biochemical composition of the incised paw.
36414090	7	65	theme	necrosis	1070:1077	arg1	TNF-α					1089:1093	TNF-α	1089:1093	TNF-α	1089:1093	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	7	65	theme	necrosis	1070:1077	arg1	interleukin-1β					1097:1110	interleukin-1β	1097:1110	interleukin-1β (IL-1β)	1097:1118	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	7	65	theme	necrosis	1070:1077	arg1	factor-α					1079:1086	tumor necrosis factor-α	1064:1086	tumor necrosis factor-α (TNF-α)	1064:1094	Magnolol strongly attenuated post-incision inducing the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and inhibited deoxyribonucleic acid (DNA) fragmentation.
36414090	9	66	theme	increase	1343:1350	arg1	expression					1352:1361	post-incisional increase expression	1327:1361	post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4)	1327:1482	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	0	67	theme	TLR4/NF-κB	69:78	arg1	signaling					80:88	TLR4/NF-κB signaling	69:88	TLR4/NF-κB signaling in a postoperative pain model	69:118	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	1	68	theme	post-incisional	198:212	arg1	pain					239:242	post-incisional inflammatory nociceptive pain	198:242	post-incisional inflammatory nociceptive pain	198:242	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	5	69	theme	thermal	807:813	arg1	hyperalgesia					815:826	thermal hyperalgesia	807:826	thermal hyperalgesia	807:826	KEY FINDINGS The results indicate that magnolol significantly suppressed post-incision-induced mechanical allodynia, thermal hyperalgesia, and paw edema.
36414090	9	70	theme	receptor	1376:1383	arg1	vanilloid					1395:1403	transient receptor potential vanilloid 1	1366:1405	transient receptor potential vanilloid 1 (TRPV1)	1366:1413	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	70	theme	receptor	1376:1383	arg1	TRPV1					1408:1412	TRPV1	1408:1412	TRPV1	1408:1412	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	5	71	dep	indicate	715:722	arg1	FINDINGS					694:701	KEY FINDINGS	690:701	KEY FINDINGS	690:701	KEY FINDINGS The results indicate that magnolol significantly suppressed post-incision-induced mechanical allodynia, thermal hyperalgesia, and paw edema.
36414090	0	72	theme	postoperative	95:107	arg1	model					114:118	a postoperative pain model	93:118	a postoperative pain model	93:118	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	9	73	theme	vanilloid	1395:1403	arg1	cyclooxygenase-2					1556:1571	cyclooxygenase-2	1556:1571	cyclooxygenase-2 (COX-2)	1556:1579	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	73	theme	vanilloid	1395:1403	arg1	synthase					1608:1615	inducible nitric oxide synthase	1585:1615	inducible nitric oxide synthase (iNOS)	1585:1622	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	73	theme	vanilloid	1395:1403	arg1	COX-2					1574:1578	COX-2	1574:1578	COX-2	1574:1578	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	73	theme	vanilloid	1395:1403	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	73	theme	vanilloid	1395:1403	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	73	theme	vanilloid	1395:1403	arg1	iNOS					1618:1621	iNOS	1618:1621	iNOS	1618:1621	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	73	theme	vanilloid	1395:1403	arg1	expression					1352:1361	post-incisional increase expression	1327:1361	post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4)	1327:1482	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	5	74	theme	post-incision-induced	763:783	arg1	allodynia					796:804	post-incision-induced mechanical allodynia	763:804	post-incision-induced mechanical allodynia	763:804	KEY FINDINGS The results indicate that magnolol significantly suppressed post-incision-induced mechanical allodynia, thermal hyperalgesia, and paw edema.
36414090	0	75	from	inhibition	41:50	arg1	model					114:118	a postoperative pain model	93:118	a postoperative pain model	93:118	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	1	76	dep	AIMS	121:124	arg1	explored					144:151	explored	144:151	explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain	144:242	AIMS The current study explored the anti-nociceptive activity of magnolol in post-incisional inflammatory nociceptive pain.
36414090	3	77	theme	surgery	459:465	arg1	model					433:437	an in-vivo model	422:437	an in-vivo model of planter incision surgery	422:465	Next, an in-vivo model of planter incision surgery was established in BALB/c mice.
36414090	9	78	theme	nitric	1595:1600	arg1	iNOS					1618:1621	iNOS	1618:1621	iNOS	1618:1621	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	78	theme	nitric	1595:1600	arg1	synthase					1608:1615	inducible nitric oxide synthase	1585:1615	inducible nitric oxide synthase (iNOS)	1585:1622	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	2	79	dep	confirmed	353:361	arg1	Preliminary					258:268	Preliminary	258:268	Preliminary	258:268	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	2	80	theme	PC12	404:407	arg1	cells					409:413	hydrogen peroxide (H2O2)-induced PC12 cells	371:413	hydrogen peroxide (H2O2)-induced PC12 cells	371:413	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	0	81	theme	magnolol	28:35	arg1	effects					17:23	Anti-nociceptive effects	0:23	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.	0:119	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	9	82	theme	purinergic	1416:1425	arg1	nociceptors					1433:1443	purinergic (P2Y) nociceptors	1416:1443	purinergic (P2Y) nociceptors	1416:1443	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	3	83	theme	BALB/c	486:491	arg1	mice					493:496	BALB/c mice	486:496	BALB/c mice	486:496	Next, an in-vivo model of planter incision surgery was established in BALB/c mice.
36414090	9	84	theme	P2Y	1428:1430	arg1	nociceptors					1433:1443	purinergic (P2Y) nociceptors	1416:1443	purinergic (P2Y) nociceptors	1416:1443	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	5	85	theme	mechanical	785:794	arg1	allodynia					796:804	post-incision-induced mechanical allodynia	763:804	post-incision-induced mechanical allodynia	763:804	KEY FINDINGS The results indicate that magnolol significantly suppressed post-incision-induced mechanical allodynia, thermal hyperalgesia, and paw edema.
36414090	0	86	theme	TRPV1/P2Y	55:63	arg1	signaling					80:88	TLR4/NF-κB signaling	69:88	TLR4/NF-κB signaling in a postoperative pain model	69:118	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	0	86	theme	TRPV1/P2Y	55:63	arg1	inhibition					41:50	inhibition	41:50	inhibition of TRPV1/P2Y	41:63	Anti-nociceptive effects of magnolol via inhibition of TRPV1/P2Y and TLR4/NF-κB signaling in a postoperative pain model.
36414090	9	87	theme	nociceptors	1433:1443	arg1	cyclooxygenase-2					1556:1571	cyclooxygenase-2	1556:1571	cyclooxygenase-2 (COX-2)	1556:1579	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	87	theme	nociceptors	1433:1443	arg1	synthase					1608:1615	inducible nitric oxide synthase	1585:1615	inducible nitric oxide synthase (iNOS)	1585:1622	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	87	theme	nociceptors	1433:1443	arg1	COX-2					1574:1578	COX-2	1574:1578	COX-2	1574:1578	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	87	theme	nociceptors	1433:1443	arg1	NF-κB					1548:1552	NF-κB	1548:1552	NF-κB	1548:1552	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	87	theme	nociceptors	1433:1443	arg1	enhancer					1518:1525	nuclear factor kappa light chain enhancer	1485:1525	nuclear factor kappa light chain enhancer of activated B cell (NF-κB)	1485:1553	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	87	theme	nociceptors	1433:1443	arg1	iNOS					1618:1621	iNOS	1618:1621	iNOS	1618:1621	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	87	theme	nociceptors	1433:1443	arg1	expression					1352:1361	post-incisional increase expression	1327:1361	post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4)	1327:1482	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	2	88	theme	hydrogen	371:378	arg1	peroxide					380:387	hydrogen peroxide	371:387	hydrogen peroxide	371:387	MAIN METHODS Preliminary, the anti-inflammatory, antioxidant, and cytoprotective potential of magnolol were confirmed against hydrogen peroxide (H2O2)-induced PC12 cells.
36414090	6	89	theme	nitric	897:902	arg1	NO					911:912	NO	911:912	NO	911:912	Magnolol promisingly inhibited post-incision induces nitric oxide (NO), malondialdehyde (MDA), eosinophil peroxidase (EPO), and neutrophil infiltration.
36414090	6	89	theme	nitric	897:902	arg1	oxide					904:908	nitric oxide	897:908	nitric oxide (NO)	897:913	Magnolol promisingly inhibited post-incision induces nitric oxide (NO), malondialdehyde (MDA), eosinophil peroxidase (EPO), and neutrophil infiltration.
36414090	9	90	theme	toll-like	1456:1464	arg1	receptor					1466:1473	toll-like receptor 4	1456:1475	toll-like receptor 4 (TLR4)	1456:1482	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
36414090	9	90	theme	toll-like	1456:1464	arg1	TLR4					1478:1481	TLR4	1478:1481	TLR4	1478:1481	Magnolol markedly down-regulated post-incisional increase expression of transient receptor potential vanilloid 1 (TRPV1), purinergic (P2Y) nociceptors as well as toll-like receptor 4 (TLR4), nuclear factor kappa light chain enhancer of activated B cell (NF-κB), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) while upregulating the expression of inhibitor of nuclear kappa B alpha (IκB-α).
35688258	7	0	from	cytokines	1448:1456	arg1	weight					1305:1310	weight	1305:1310	weight	1305:1310	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	0	from	cytokines	1448:1456	arg1	length					1294:1299	the length	1290:1299	the length	1290:1299	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	1	theme	markers	1426:1432	arg1	morphology					1348:1357	pathological morphology	1335:1357	pathological morphology by hematoxylin and eosin (HE) staining	1335:1396	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	1	theme	markers	1426:1432	arg1	factor-alpha					1477:1488	tumor necrosis factor-alpha	1462:1488	tumor necrosis factor-alpha (TNF-α)	1462:1496	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	1	theme	markers	1426:1432	arg1	cytokines					1448:1456	inflammatory cytokines	1435:1456	inflammatory cytokines	1435:1456	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	1	theme	markers	1426:1432	arg1	TNF-α					1491:1495	TNF-α	1491:1495	TNF-α	1491:1495	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	1	theme	markers	1426:1432	arg1	determination					1399:1411	determination	1399:1411	determination of oxidative markers	1399:1432	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	1	theme	markers	1426:1432	arg1	inspection					1276:1285	visual inspection	1269:1285	visual inspection in the length and weight of colons and spleens	1269:1332	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	9	2	theme	main	1812:1815	arg1	components					1817:1826	The main components	1808:1826	RESULTS The main components in HS	1800:1832	RESULTS The main components in HS were found to be polycyclic polyprenylated acylphloroglucinols (PPAPs).
35688258	8	3	theme	expression	1554:1563	arg1	levels					1565:1570	Gene expression levels	1549:1570	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4	1549:1626	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	3	4	theme	ulcerative	712:721	arg1	UC					732:733	UC	732:733	UC	732:733	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	3	4	theme	ulcerative	712:721	arg1	colitis					723:729	ulcerative colitis	712:729	ulcerative colitis (UC)	712:734	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	8	5	theme	Western	1786:1792	arg1	blot					1794:1797	Western blot	1786:1797	Western blot	1786:1797	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	2	6	theme	anti-inflammatory	388:404	arg1	effects					406:412	its anti-inflammatory effects	384:412	its anti-inflammatory effects	384:412	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	8	7	theme	pathway	1761:1767	arg1	proteins					1709:1716	colonic tight junction proteins	1686:1716	colonic tight junction proteins	1686:1716	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	8	7	theme	pathway	1761:1767	arg1	signals					1731:1737	relative signals	1722:1737	relative signals of PKA/CREB signaling pathway	1722:1767	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	7	8	theme	eosin	1378:1382	arg1	staining					1389:1396	hematoxylin and eosin (HE) staining	1362:1396	hematoxylin and eosin (HE) staining	1362:1396	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	18	9	from	application	3061:3071	arg1	treatment					3102:3110	the treatment	3098:3110	the treatment of diarrhea and dysentery	3098:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	14	10	theme	PDE4	2491:2494	arg1	level					2482:2486	the protein level	2470:2486	the protein level of PDE4	2470:2494	Besides, HS significantly inhibited the protein level of PDE4 and decreased the expressions of PKA and phosphorylated CREB.
35688258	3	11	dep	-induced	703:710	arg1	UC					732:733	UC	732:733	UC	732:733	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	3	11	dep	-induced	703:710	arg1	colitis					723:729	ulcerative colitis	712:729	ulcerative colitis (UC)	712:734	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	7	12	from	factor-alpha	1477:1488	arg1	weight					1305:1310	weight	1305:1310	weight	1305:1310	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	12	from	factor-alpha	1477:1488	arg1	length					1294:1299	the length	1290:1299	the length	1290:1299	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	13	theme	hematoxylin	1362:1372	arg1	staining					1389:1396	hematoxylin and eosin (HE) staining	1362:1396	hematoxylin and eosin (HE) staining	1362:1396	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	17	14	theme	PDE4	2943:2946	arg1	protection					2881:2890	the protection	2877:2890	the protection for intestinal barrier function	2877:2922	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	17	14	theme	PDE4	2943:2946	arg1	inhibition					2929:2938	the inhibition	2925:2938	the inhibition of PDE4	2925:2946	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	17	14	theme	PDE4	2943:2946	arg1	improvement					2841:2851	the improvement	2837:2851	the improvement to inflammatory status	2837:2874	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	17	14	theme	PDE4	2943:2946	arg1	activation					2957:2966	the activation	2953:2966	the activation of PKA/CREB signaling pathway	2953:2996	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	1	15	theme	traditional	199:209	arg1	medicine					218:225	a traditional herbal medicine	197:225	a traditional herbal medicine with various pharmacological properties	197:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	15	theme	traditional	199:209	arg1	Hance					177:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance	126:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao)	126:194	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	7	16	theme	morphology	1348:1357	arg1	levels					1498:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels	1269:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index	1269:1546	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	17	17	theme	signaling	2980:2988	arg1	pathway					2990:2996	PKA/CREB signaling pathway	2971:2996	PKA/CREB signaling pathway	2971:2996	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	3	18	theme	therapeutic	609:619	arg1	effects					621:627	the therapeutic effects	605:627	the therapeutic effects	605:627	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	5	19	theme	inflammatory	877:888	arg1	state					890:894	The inflammatory state	873:894	The inflammatory state of UC mice	873:905	The inflammatory state of UC mice was caused by 3% DSS once daily for seven days.
35688258	9	20	theme	polycyclic	1851:1860	arg1	PPAPs					1898:1902	PPAPs	1898:1902	PPAPs	1898:1902	RESULTS The main components in HS were found to be polycyclic polyprenylated acylphloroglucinols (PPAPs).
35688258	9	20	theme	polycyclic	1851:1860	arg1	acylphloroglucinols					1877:1895	polycyclic polyprenylated acylphloroglucinols	1851:1895	polycyclic polyprenylated acylphloroglucinols (PPAPs)	1851:1903	RESULTS The main components in HS were found to be polycyclic polyprenylated acylphloroglucinols (PPAPs).
35688258	9	21	dep	RESULTS	1800:1806	arg1	components					1817:1826	The main components	1808:1826	RESULTS The main components in HS	1800:1832	RESULTS The main components in HS were found to be polycyclic polyprenylated acylphloroglucinols (PPAPs).
35688258	16	22	theme	protective	2760:2769	arg1	effect					2771:2776	significantly protective effect	2746:2776	significantly protective effect	2746:2776	For the first time, this study reveals HS is not toxic in a single dose and exert significantly protective effect in DSS-colitis mice.
35688258	17	23	theme	underlying	2803:2812	arg1	mechanisms					2814:2823	The underlying mechanisms	2799:2823	The underlying mechanisms	2799:2823	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	3	24	dep	H.	657:658	arg1	sampsonii					660:668	H. sampsonii	657:668	H. sampsonii	657:668	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	7	25	from	determination	1399:1411	arg1	weight					1305:1310	weight	1305:1310	weight	1305:1310	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	25	from	determination	1399:1411	arg1	length					1294:1299	the length	1290:1299	the length	1290:1299	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	10	26	theme	colons	2072:2077	arg1	injuries					2060:2067	histological injuries	2047:2067	histological injuries of colons	2047:2077	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	10	26	theme	colons	2072:2077	arg1	weight					2020:2025	body weight	2015:2025	body weight	2015:2025	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	10	26	theme	colons	2072:2077	arg1	lengths					2034:2040	colon lengths	2028:2040	colon lengths	2028:2040	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	10	27	theme	DSS-stimulated	1931:1944	arg1	symptoms					1962:1969	DSS-stimulated UC-like lesions symptoms	1931:1969	DSS-stimulated UC-like lesions symptoms	1931:1969	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	14	28	theme	CREB	2552:2555	arg1	expressions					2514:2524	the expressions	2510:2524	the expressions of PKA and phosphorylated CREB	2510:2555	Besides, HS significantly inhibited the protein level of PDE4 and decreased the expressions of PKA and phosphorylated CREB.
35688258	13	29	theme	protein	2404:2410	arg1	expression					2364:2373	the protein expression	2352:2373	the protein expression of tight junction-associated protein (ZO-1 and occluding)	2352:2431	Furthermore, HS restored the protein expression of tight junction-associated protein (ZO-1 and occluding).
35688258	8	30	theme	tight	1694:1698	arg1	proteins					1709:1716	colonic tight junction proteins	1686:1716	colonic tight junction proteins	1686:1716	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	1	31	theme	Hypericum	157:165	arg1	Yuanbaocao					184:193	Yuanbaocao	184:193	Yuanbaocao	184:193	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	31	theme	Hypericum	157:165	arg1	Hance					177:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance	126:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao)	126:194	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	31	theme	Hypericum	157:165	arg1	medicine					218:225	a traditional herbal medicine	197:225	a traditional herbal medicine with various pharmacological properties	197:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	7	32	theme	spleen	1535:1540	arg1	index					1542:1546	spleen index	1535:1546	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index	1269:1546	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	9	33	from	components	1817:1826	arg1	HS					1831:1832	HS	1831:1832	HS	1831:1832	RESULTS The main components in HS were found to be polycyclic polyprenylated acylphloroglucinols (PPAPs).
35688258	8	34	theme	proteins	1709:1716	arg1	expression					1672:1681	the expression	1668:1681	the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway	1668:1767	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	4	35	dep	MATERIALS	748:756	arg1	ingredients					780:790	The major ingredients	770:790	The major ingredients	770:790	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	7	36	from	morphology	1348:1357	arg1	weight					1305:1310	weight	1305:1310	weight	1305:1310	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	36	from	morphology	1348:1357	arg1	length					1294:1299	the length	1290:1299	the length	1290:1299	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	16	37	from	dose	2731:2734	arg1	toxic					2713:2717	toxic	2713:2717	toxic	2713:2717	For the first time, this study reveals HS is not toxic in a single dose and exert significantly protective effect in DSS-colitis mice.
35688258	10	38	theme	lesions	1954:1960	arg1	symptoms					1962:1969	DSS-stimulated UC-like lesions symptoms	1931:1969	DSS-stimulated UC-like lesions symptoms	1931:1969	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	7	39	theme	pathological	1156:1167	arg1	observation					1169:1179	colonic pathological observation	1148:1179	colonic pathological observation	1148:1179	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	3	40	theme	H.	657:658	arg1	effects					621:627	the therapeutic effects	605:627	the therapeutic effects	605:627	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	3	40	theme	H.	657:658	arg1	mechanisms					643:652	molecular mechanisms	633:652	molecular mechanisms	633:652	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	8	41	theme	relative	1722:1729	arg1	signals					1731:1737	relative signals	1722:1737	relative signals of PKA/CREB signaling pathway	1722:1767	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	11	42	theme	mRNA	2155:2158	arg1	level					2160:2164	the mRNA level	2151:2164	the mRNA level of IL-10	2151:2173	HS reduced the accumulation of pro-inflammatory cytokines and improved the mRNA level of IL-10.
35688258	0	43	from	inflammation	30:41	arg1	mice					46:49	mice	46:49	mice with ulcerative colitis	46:73	Hypericumsampsonii attenuates inflammation in mice with ulcerative colitis via regulation of PDE4/PKA/CREB signaling pathway.
35688258	6	44	from	mice	981:984	arg1	group					1007:1011	the positive drug group	989:1011	the positive drug group	989:1011	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	6	44	from	mice	981:984	arg1	groups					1033:1038	the other three groups	1017:1038	the other three groups	1017:1038	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	8	45	theme	PKA/CREB	1742:1749	arg1	pathway					1761:1767	PKA/CREB signaling pathway	1742:1767	PKA/CREB signaling pathway	1742:1767	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	10	46	theme	significant	1989:1999	arg1	recovery					2001:2008	a significant recovery	1987:2008	a significant recovery from body weight, colon lengths, and histological injuries of colons	1987:2077	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	5	47	theme	mice	902:905	arg1	state					890:894	The inflammatory state	873:894	The inflammatory state of UC mice	873:905	The inflammatory state of UC mice was caused by 3% DSS once daily for seven days.
35688258	17	48	theme	barrier	2907:2913	arg1	function					2915:2922	intestinal barrier function	2896:2922	intestinal barrier function	2896:2922	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	1	49	theme	ETHNOPHARMACOLOGICAL	126:145	arg1	Yuanbaocao					184:193	Yuanbaocao	184:193	Yuanbaocao	184:193	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	49	theme	ETHNOPHARMACOLOGICAL	126:145	arg1	Hance					177:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance	126:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao)	126:194	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	49	theme	ETHNOPHARMACOLOGICAL	126:145	arg1	medicine					218:225	a traditional herbal medicine	197:225	a traditional herbal medicine with various pharmacological properties	197:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	0	50	theme	signaling	107:115	arg1	pathway					117:123	PDE4/PKA/CREB signaling pathway	93:123	PDE4/PKA/CREB signaling pathway	93:123	Hypericumsampsonii attenuates inflammation in mice with ulcerative colitis via regulation of PDE4/PKA/CREB signaling pathway.
35688258	8	51	theme	signaling	1751:1759	arg1	pathway					1761:1767	PKA/CREB signaling pathway	1742:1767	PKA/CREB signaling pathway	1742:1767	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	12	52	theme	colonic	2196:2202	arg1	levels					2220:2225	the colonic mRNA expression levels	2192:2225	the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2	2192:2257	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	10	53	from	weight	2020:2025	arg1	recovery					2001:2008	a significant recovery	1987:2008	a significant recovery from body weight, colon lengths, and histological injuries of colons	1987:2077	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	13	54	theme	tight	2378:2382	arg1	protein					2404:2410	tight junction-associated protein	2378:2410	tight junction-associated protein (ZO-1 and occluding)	2378:2431	Furthermore, HS restored the protein expression of tight junction-associated protein (ZO-1 and occluding).
35688258	2	55	theme	action	499:504	arg1	unclear					547:553	unclear	547:553	unclear	547:553	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	2	55	theme	action	499:504	arg1	mechanisms					485:494	the mechanisms	481:494	the mechanisms of action for the treatment of enteritis	481:535	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	12	56	theme	expression	2209:2218	arg1	levels					2220:2225	the colonic mRNA expression levels	2192:2225	the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2	2192:2257	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	15	57	theme	anti-UC	2619:2625	arg1	effect					2627:2632	anti-UC effect	2619:2632	anti-UC effect	2619:2632	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	3	58	dep	AIMS	556:559	arg1	aims					585:588	aims	585:588	aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	585:745	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	7	59	theme	visual	1269:1274	arg1	inspection					1276:1285	visual inspection	1269:1285	visual inspection in the length and weight of colons and spleens	1269:1332	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	16	60	theme	single	2724:2729	arg1	dose					2731:2734	a single dose	2722:2734	a single dose	2722:2734	For the first time, this study reveals HS is not toxic in a single dose and exert significantly protective effect in DSS-colitis mice.
35688258	7	61	theme	factor-alpha	1477:1488	arg1	levels					1498:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels	1269:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index	1269:1546	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	5	62	theme	daily	933:937	arg1	DSS					924:926	3% DSS	921:926	3% DSS once daily for seven days	921:952	The inflammatory state of UC mice was caused by 3% DSS once daily for seven days.
35688258	16	63	theme	first	2672:2676	arg1	time					2678:2681	the first time	2668:2681	the first time	2668:2681	For the first time, this study reveals HS is not toxic in a single dose and exert significantly protective effect in DSS-colitis mice.
35688258	3	64	from	mechanisms	643:652	arg1	model					741:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	17	65	theme	inflammatory	2856:2867	arg1	status					2869:2874	inflammatory status	2856:2874	inflammatory status	2856:2874	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	15	66	theme	Hance	2657:2661	arg1	composition					2603:2613	main composition	2598:2613	main composition	2598:2613	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	15	66	theme	Hance	2657:2661	arg1	effect					2627:2632	anti-UC effect	2619:2632	anti-UC effect	2619:2632	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	7	67	theme	tumor	1462:1466	arg1	TNF-α					1491:1495	TNF-α	1491:1495	TNF-α	1491:1495	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	67	theme	tumor	1462:1466	arg1	factor-alpha					1477:1488	tumor necrosis factor-alpha	1462:1488	tumor necrosis factor-alpha (TNF-α)	1462:1496	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	2	68	from	constituents	442:453	arg1	H.					458:459	H.	458:459	H.	458:459	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	6	69	theme	positive	993:1000	arg1	group					1007:1011	the positive drug group	989:1011	the positive drug group	989:1011	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	11	70	theme	cytokines	2128:2136	arg1	accumulation					2095:2106	the accumulation	2091:2106	the accumulation of pro-inflammatory cytokines	2091:2136	HS reduced the accumulation of pro-inflammatory cytokines and improved the mRNA level of IL-10.
35688258	0	71	theme	ulcerative	56:65	arg1	colitis					67:73	ulcerative colitis	56:73	ulcerative colitis	56:73	Hypericumsampsonii attenuates inflammation in mice with ulcerative colitis via regulation of PDE4/PKA/CREB signaling pathway.
35688258	1	72	theme	years	347:351	arg1	hundreds					335:342	hundreds	335:342	hundreds of years	335:351	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	73	with	medicine	218:225	arg1	properties					256:265	various pharmacological properties	232:265	various pharmacological properties	232:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	7	74	theme	cytokines	1448:1456	arg1	levels					1498:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels	1269:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index	1269:1546	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	18	75	theme	H.	3076:3077	arg1	application					3061:3071	the traditional application	3045:3071	the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery	3045:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	3	76	theme	-induced	703:710	arg1	model					741:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	4	77	theme	acetate	805:811	arg1	extract					813:819	the ethyl acetate extract	795:819	the ethyl acetate extract (HS)	795:824	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	4	77	theme	acetate	805:811	arg1	HS					822:823	HS	822:823	HS	822:823	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	8	78	theme	inflammatory	1575:1586	arg1	cytokines					1588:1596	inflammatory cytokines	1575:1596	inflammatory cytokines	1575:1596	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	12	79	theme	dose-dependent	2304:2317	arg1	manner					2319:2324	a dose-dependent manner	2302:2324	a dose-dependent manner	2302:2324	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	7	80	theme	therapeutic	1235:1245	arg1	evaluation					1247:1256	therapeutic evaluation	1235:1256	therapeutic evaluation	1235:1256	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	2	81	dep	H.	458:459	arg1	sampsonii					461:469	H. sampsonii	458:469	H. sampsonii	458:469	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	2	82	theme	corresponding	418:430	arg1	constituents					442:453	corresponding bioactive constituents	418:453	corresponding bioactive constituents in H. sampsonii	418:469	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	16	83	from	toxic	2713:2717	arg1	dose					2731:2734	a single dose	2722:2734	a single dose	2722:2734	For the first time, this study reveals HS is not toxic in a single dose and exert significantly protective effect in DSS-colitis mice.
35688258	7	84	theme	oxidative	1416:1424	arg1	markers					1426:1432	oxidative markers	1416:1432	oxidative markers	1416:1432	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	85	from	inspection	1276:1285	arg1	weight					1305:1310	weight	1305:1310	weight	1305:1310	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	85	from	inspection	1276:1285	arg1	length					1294:1299	the length	1290:1299	the length	1290:1299	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	86	theme	molecular	1189:1197	arg1	index					1210:1214	the molecular biological index	1185:1214	the molecular biological index	1185:1214	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	17	87	theme	PKA/CREB	2971:2978	arg1	pathway					2990:2996	PKA/CREB signaling pathway	2971:2996	PKA/CREB signaling pathway	2971:2996	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	0	88	with	mice	46:49	arg1	colitis					67:73	ulcerative colitis	56:73	ulcerative colitis	56:73	Hypericumsampsonii attenuates inflammation in mice with ulcerative colitis via regulation of PDE4/PKA/CREB signaling pathway.
35688258	8	89	theme	enzymes	1611:1617	arg1	levels					1565:1570	Gene expression levels	1549:1570	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4	1549:1626	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	7	90	theme	determination	1399:1411	arg1	levels					1498:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels	1269:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index	1269:1546	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	10	91	theme	colon	2028:2032	arg1	lengths					2034:2040	colon lengths	2028:2040	colon lengths	2028:2040	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	8	92	theme	Gene	1549:1552	arg1	levels					1565:1570	Gene expression levels	1549:1570	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4	1549:1626	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	17	93	theme	pathway	2990:2996	arg1	protection					2881:2890	the protection	2877:2890	the protection for intestinal barrier function	2877:2922	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	17	93	theme	pathway	2990:2996	arg1	inhibition					2929:2938	the inhibition	2925:2938	the inhibition of PDE4	2925:2946	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	17	93	theme	pathway	2990:2996	arg1	improvement					2841:2851	the improvement	2837:2851	the improvement to inflammatory status	2837:2874	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	17	93	theme	pathway	2990:2996	arg1	activation					2957:2966	the activation	2953:2966	the activation of PKA/CREB signaling pathway	2953:2996	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	8	94	theme	PDE4	1623:1626	arg1	levels					1565:1570	Gene expression levels	1549:1570	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4	1549:1626	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	3	95	theme	STUDY	568:572	arg1	AIMS					556:559	AIMS	556:559	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.	556:746	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	18	96	theme	experimental	3022:3033	arg1	basis					3035:3039	an experimental basis	3019:3039	an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery	3019:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	15	97	theme	Hypericum	2637:2645	arg1	Hance					2657:2661	Hypericum sampsonii Hance	2637:2661	Hypericum sampsonii Hance	2637:2661	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	10	98	theme	histological	2047:2058	arg1	injuries					2060:2067	histological injuries	2047:2067	histological injuries of colons	2047:2077	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	18	99	theme	traditional	3049:3059	arg1	application					3061:3071	the traditional application	3045:3071	the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery	3045:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	3	100	theme	mice	736:739	arg1	model					741:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	1	101	from	enteritis	312:320	arg1	China					325:329	China	325:329	China	325:329	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	102	theme	herbal	211:216	arg1	medicine					218:225	a traditional herbal medicine	197:225	a traditional herbal medicine with various pharmacological properties	197:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	102	theme	herbal	211:216	arg1	Hance					177:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance	126:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao)	126:194	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	103	used	used	285:288	arg2	medicine					218:225	a traditional herbal medicine	197:225	a traditional herbal medicine with various pharmacological properties	197:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	103	used	used	285:288	arg2	Yuanbaocao					184:193	Yuanbaocao	184:193	Yuanbaocao	184:193	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	103	used	used	285:288	arg2	Hance					177:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance	126:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao)	126:194	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	104	from	diarrhea	299:306	arg1	China					325:329	China	325:329	China	325:329	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	4	105	dep	H.	829:830	arg1	sampsonii					832:840	H. sampsonii	829:840	H. sampsonii	829:840	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	7	106	with	treatment	1109:1117	arg1	5-ASA					1130:1134	5-ASA	1130:1134	5-ASA	1130:1134	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	106	with	treatment	1109:1117	arg1	HS					1124:1125	HS	1124:1125	HS	1124:1125	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	107	theme	pathological	1335:1346	arg1	morphology					1348:1357	pathological morphology	1335:1357	pathological morphology by hematoxylin and eosin (HE) staining	1335:1396	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	9	108	theme	polyprenylated	1862:1875	arg1	PPAPs					1898:1902	PPAPs	1898:1902	PPAPs	1898:1902	RESULTS The main components in HS were found to be polycyclic polyprenylated acylphloroglucinols (PPAPs).
35688258	9	108	theme	polyprenylated	1862:1875	arg1	acylphloroglucinols					1877:1895	polycyclic polyprenylated acylphloroglucinols	1851:1895	polycyclic polyprenylated acylphloroglucinols (PPAPs)	1851:1903	RESULTS The main components in HS were found to be polycyclic polyprenylated acylphloroglucinols (PPAPs).
35688258	18	109	dep	H.	3076:3077	arg1	Hance					3089:3093	Hance	3089:3093	Hance	3089:3093	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	18	109	dep	H.	3076:3077	arg1	sampsonii					3079:3087	H. sampsonii Hance in the treatment of diarrhea and dysentery	3076:3136	H. sampsonii Hance in the treatment of diarrhea and dysentery	3076:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	16	110	theme	DSS-colitis	2781:2791	arg1	mice					2793:2796	DSS-colitis mice	2781:2796	DSS-colitis mice	2781:2796	For the first time, this study reveals HS is not toxic in a single dose and exert significantly protective effect in DSS-colitis mice.
35688258	3	111	theme	molecular	633:641	arg1	mechanisms					643:652	molecular mechanisms	633:652	molecular mechanisms	633:652	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	1	112	theme	pharmacological	240:254	arg1	properties					256:265	various pharmacological properties	232:265	various pharmacological properties	232:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	7	113	theme	spleens	1326:1332	arg1	weight					1305:1310	weight	1305:1310	weight	1305:1310	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	113	theme	spleens	1326:1332	arg1	length					1294:1299	the length	1290:1299	the length	1290:1299	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	13	114	theme	junction-associated	2384:2402	arg1	protein					2404:2410	tight junction-associated protein	2378:2410	tight junction-associated protein (ZO-1 and occluding)	2378:2431	Furthermore, HS restored the protein expression of tight junction-associated protein (ZO-1 and occluding).
35688258	1	115	theme	RELEVANCE	147:155	arg1	Yuanbaocao					184:193	Yuanbaocao	184:193	Yuanbaocao	184:193	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	115	theme	RELEVANCE	147:155	arg1	Hance					177:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance	126:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao)	126:194	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	115	theme	RELEVANCE	147:155	arg1	medicine					218:225	a traditional herbal medicine	197:225	a traditional herbal medicine with various pharmacological properties	197:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	14	116	theme	phosphorylated	2537:2550	arg1	CREB					2552:2555	phosphorylated CREB	2537:2555	phosphorylated CREB	2537:2555	Besides, HS significantly inhibited the protein level of PDE4 and decreased the expressions of PKA and phosphorylated CREB.
35688258	12	117	theme	iNOS	2244:2247	arg1	levels					2220:2225	the colonic mRNA expression levels	2192:2225	the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2	2192:2257	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	7	118	theme	colons	1315:1320	arg1	weight					1305:1310	weight	1305:1310	weight	1305:1310	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	118	theme	colons	1315:1320	arg1	length					1294:1299	the length	1290:1299	the length	1290:1299	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	17	119	theme	intestinal	2896:2905	arg1	function					2915:2922	intestinal barrier function	2896:2922	intestinal barrier function	2896:2922	The underlying mechanisms may involve the improvement to inflammatory status, the protection for intestinal barrier function, the inhibition of PDE4, and the activation of PKA/CREB signaling pathway.
35688258	8	120	theme	colonic	1686:1692	arg1	proteins					1709:1716	colonic tight junction proteins	1686:1716	colonic tight junction proteins	1686:1716	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	1	121	theme	sampsonii	167:175	arg1	Yuanbaocao					184:193	Yuanbaocao	184:193	Yuanbaocao	184:193	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	121	theme	sampsonii	167:175	arg1	Hance					177:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance	126:181	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao)	126:194	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	1	121	theme	sampsonii	167:175	arg1	medicine					218:225	a traditional herbal medicine	197:225	a traditional herbal medicine with various pharmacological properties	197:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	14	122	theme	PKA	2529:2531	arg1	expressions					2514:2524	the expressions	2510:2524	the expressions of PKA and phosphorylated CREB	2510:2555	Besides, HS significantly inhibited the protein level of PDE4 and decreased the expressions of PKA and phosphorylated CREB.
35688258	12	123	theme	COX-2	2253:2257	arg1	levels					2220:2225	the colonic mRNA expression levels	2192:2225	the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2	2192:2257	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	7	124	theme	biological	1199:1208	arg1	index					1210:1214	the molecular biological index	1185:1214	the molecular biological index	1185:1214	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	125	theme	colonic	1148:1154	arg1	observation					1169:1179	colonic pathological observation	1148:1179	colonic pathological observation	1148:1179	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	8	126	theme	junction	1700:1707	arg1	proteins					1709:1716	colonic tight junction proteins	1686:1716	colonic tight junction proteins	1686:1716	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	7	127	theme	colonic	1508:1514	arg1	tissues					1516:1522	colonic tissues	1508:1522	colonic tissues	1508:1522	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	11	128	theme	IL-10	2169:2173	arg1	level					2160:2164	the mRNA level	2151:2164	the mRNA level of IL-10	2151:2173	HS reduced the accumulation of pro-inflammatory cytokines and improved the mRNA level of IL-10.
35688258	12	129	theme	IL-1β	2230:2234	arg1	levels					2220:2225	the colonic mRNA expression levels	2192:2225	the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2	2192:2257	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	10	130	theme	UC-like	1946:1952	arg1	symptoms					1962:1969	DSS-stimulated UC-like lesions symptoms	1931:1969	DSS-stimulated UC-like lesions symptoms	1931:1969	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	0	131	theme	PDE4/PKA/CREB	93:105	arg1	pathway					117:123	PDE4/PKA/CREB signaling pathway	93:123	PDE4/PKA/CREB signaling pathway	93:123	Hypericumsampsonii attenuates inflammation in mice with ulcerative colitis via regulation of PDE4/PKA/CREB signaling pathway.
35688258	5	132	theme	UC	899:900	arg1	mice					902:905	UC mice	899:905	UC mice	899:905	The inflammatory state of UC mice was caused by 3% DSS once daily for seven days.
35688258	2	133	theme	enteritis	527:535	arg1	treatment					514:522	the treatment	510:522	the treatment of enteritis	510:535	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	12	134	theme	IL-17	2237:2241	arg1	levels					2220:2225	the colonic mRNA expression levels	2192:2225	the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2	2192:2257	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	0	135	theme	pathway	117:123	arg1	regulation					79:88	regulation	79:88	regulation of PDE4/PKA/CREB signaling pathway	79:123	Hypericumsampsonii attenuates inflammation in mice with ulcerative colitis via regulation of PDE4/PKA/CREB signaling pathway.
35688258	7	136	from	index	1542:1546	arg1	tissues					1516:1522	colonic tissues	1508:1522	colonic tissues	1508:1522	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	8	137	theme	signals	1731:1737	arg1	expression					1672:1681	the expression	1668:1681	the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway	1668:1767	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	13	138	theme	protein	2356:2362	arg1	expression					2364:2373	the protein expression	2352:2373	the protein expression of tight junction-associated protein (ZO-1 and occluding)	2352:2431	Furthermore, HS restored the protein expression of tight junction-associated protein (ZO-1 and occluding).
35688258	6	139	theme	DSS	962:964	arg1	treatment					966:974	DSS treatment	962:974	DSS treatment	962:974	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	12	140	theme	mRNA	2204:2207	arg1	levels					2220:2225	the colonic mRNA expression levels	2192:2225	the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2	2192:2257	Simultaneously, the colonic mRNA expression levels of IL-1β, IL-17, iNOS and COX-2 were all significantly suppressed by HS in a dose-dependent manner.
35688258	4	141	theme	major	774:778	arg1	ingredients					780:790	The major ingredients	770:790	The major ingredients	770:790	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	15	142	theme	first	2581:2585	arg1	work					2587:2590	the first work	2577:2590	the first work about main composition and anti-UC effect of Hypericum sampsonii Hance	2577:2661	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	15	142	theme	first	2581:2585	arg1	CONCLUSION					2558:2567	CONCLUSION	2558:2567	CONCLUSION This	2558:2572	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	1	143	theme	various	232:238	arg1	properties					256:265	various pharmacological properties	232:265	various pharmacological properties	232:265	ETHNOPHARMACOLOGICAL RELEVANCE Hypericum sampsonii Hance (Yuanbaocao), a traditional herbal medicine with various pharmacological properties, is traditionally used to treat diarrhea and enteritis in China for hundreds of years.
35688258	18	144	theme	dysentery	3128:3136	arg1	treatment					3102:3110	the treatment	3098:3110	the treatment of diarrhea and dysentery	3098:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	7	145	theme	inspection	1276:1285	arg1	levels					1498:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels	1269:1503	visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index	1269:1546	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	13	146	dep	protein	2404:2410	arg1	occluding					2422:2430	occluding	2422:2430	occluding	2422:2430	Furthermore, HS restored the protein expression of tight junction-associated protein (ZO-1 and occluding).
35688258	13	146	dep	protein	2404:2410	arg1	ZO-1					2413:2416	ZO-1	2413:2416	ZO-1	2413:2416	Furthermore, HS restored the protein expression of tight junction-associated protein (ZO-1 and occluding).
35688258	4	147	from	METHODS	762:768	arg1	H.					829:830	H.	829:830	H.	829:830	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	5	148	theme	%	922:922	arg1	DSS					924:926	3% DSS	921:926	3% DSS once daily for seven days	921:952	The inflammatory state of UC mice was caused by 3% DSS once daily for seven days.
35688258	6	149	theme	other	1021:1025	arg1	groups					1033:1038	the other three groups	1017:1038	the other three groups	1017:1038	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	11	150	theme	pro-inflammatory	2111:2126	arg1	cytokines					2128:2136	pro-inflammatory cytokines	2111:2136	pro-inflammatory cytokines	2111:2136	HS reduced the accumulation of pro-inflammatory cytokines and improved the mRNA level of IL-10.
35688258	3	151	from	effects	621:627	arg1	model					741:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model	673:745	AIMS OF THE STUDY This study aims to investigate the therapeutic effects and molecular mechanisms of H. sampsonii in a dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) mice model.
35688258	2	152	theme	bioactive	432:440	arg1	constituents					442:453	corresponding bioactive constituents	418:453	corresponding bioactive constituents in H. sampsonii	418:469	Investigations have uncovered its anti-inflammatory effects and corresponding bioactive constituents in H. sampsonii, however, the mechanisms of action for the treatment of enteritis are still unclear.
35688258	4	153	from	MATERIALS	748:756	arg1	H.					829:830	H.	829:830	H.	829:830	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	10	154	from	lengths	2034:2040	arg1	recovery					2001:2008	a significant recovery	1987:2008	a significant recovery from body weight, colon lengths, and histological injuries of colons	1987:2077	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	15	155	theme	sampsonii	2647:2655	arg1	Hance					2657:2661	Hypericum sampsonii Hance	2637:2661	Hypericum sampsonii Hance	2637:2661	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	18	156	from	treatment	3102:3110	arg1	application					3061:3071	the traditional application	3045:3071	the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery	3045:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	7	157	from	levels	1498:1503	arg1	tissues					1516:1522	colonic tissues	1508:1522	colonic tissues	1508:1522	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	14	158	theme	protein	2474:2480	arg1	level					2482:2486	the protein level	2470:2486	the protein level of PDE4	2470:2494	Besides, HS significantly inhibited the protein level of PDE4 and decreased the expressions of PKA and phosphorylated CREB.
35688258	6	159	theme	drug	1002:1005	arg1	group					1007:1011	the positive drug group	989:1011	the positive drug group	989:1011	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	15	160	dep	CONCLUSION	2558:2567	arg1	This					2569:2572	This	2569:2572	This	2569:2572	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	7	161	theme	necrosis	1468:1475	arg1	TNF-α					1491:1495	TNF-α	1491:1495	TNF-α	1491:1495	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	7	161	theme	necrosis	1468:1475	arg1	factor-alpha					1477:1488	tumor necrosis factor-alpha	1462:1488	tumor necrosis factor-alpha (TNF-α)	1462:1496	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	16	162	dep	HS	2703:2704	arg1	toxic					2713:2717	toxic	2713:2717	toxic	2713:2717	For the first time, this study reveals HS is not toxic in a single dose and exert significantly protective effect in DSS-colitis mice.
35688258	10	163	from	injuries	2060:2067	arg1	recovery					2001:2008	a significant recovery	1987:2008	a significant recovery from body weight, colon lengths, and histological injuries of colons	1987:2077	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	8	164	theme	cytokines	1588:1596	arg1	levels					1565:1570	Gene expression levels	1549:1570	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4	1549:1626	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	18	165	theme	diarrhea	3115:3122	arg1	treatment					3102:3110	the treatment	3098:3110	the treatment of diarrhea and dysentery	3098:3136	This study provided an experimental basis for the traditional application of H. sampsonii Hance in the treatment of diarrhea and dysentery.
35688258	6	166	theme	positive	1072:1079	arg1	5-ASA					1065:1069	5-ASA	1065:1069	5-ASA (positive control)	1065:1088	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	6	166	theme	positive	1072:1079	arg1	control					1081:1087	positive control	1072:1087	positive control	1072:1087	During DSS treatment, the mice in the positive drug group and the other three groups were orally administered 5-ASA (positive control) or HS daily.
35688258	10	167	theme	body	2015:2018	arg1	weight					2020:2025	body weight	2015:2025	body weight	2015:2025	HS distinctly alleviated DSS-stimulated UC-like lesions symptoms as evidenced by a significant recovery from body weight, colon lengths, and histological injuries of colons.
35688258	15	168	theme	main	2598:2601	arg1	composition					2603:2613	main composition	2598:2613	main composition	2598:2613	CONCLUSION This is the first work about main composition and anti-UC effect of Hypericum sampsonii Hance.
35688258	4	169	theme	ethyl	799:803	arg1	extract					813:819	the ethyl acetate extract	795:819	the ethyl acetate extract (HS)	795:824	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	4	169	theme	ethyl	799:803	arg1	HS					822:823	HS	822:823	HS	822:823	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	8	170	theme	antioxidant	1599:1609	arg1	enzymes					1611:1617	antioxidant enzymes	1599:1617	antioxidant enzymes	1599:1617	Gene expression levels of inflammatory cytokines, antioxidant enzymes and PDE4 were detected using kits and PCR, while the expression of colonic tight junction proteins and relative signals of PKA/CREB signaling pathway were analyzed by Western blot.
35688258	7	171	theme	inflammatory	1435:1446	arg1	cytokines					1448:1456	inflammatory cytokines	1435:1456	inflammatory cytokines	1435:1456	After treatment with HS or 5-ASA for a week, colonic pathological observation and the molecular biological index were performed for therapeutic evaluation, including visual inspection in the length and weight of colons and spleens, pathological morphology by hematoxylin and eosin (HE) staining, determination of oxidative markers, inflammatory cytokines and tumor necrosis factor-alpha (TNF-α) levels in colonic tissues as well as spleen index.
35688258	4	172	theme	extract	813:819	arg1	METHODS					762:768	METHODS	762:768	METHODS	762:768	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
35688258	4	172	theme	extract	813:819	arg1	MATERIALS					748:756	MATERIALS	748:756	MATERIALS	748:756	MATERIALS AND METHODS The major ingredients of the ethyl acetate extract (HS) in H. sampsonii were analyzed by UPLC-QTOF-MS.
36776819	5	0	theme	serum	1198:1202	arg1	levels					1204:1209	galectin serum levels	1189:1209	galectin serum levels	1189:1209	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	1	1	theme	clinical	160:167	arg1	course					169:174	clinical course	160:174	clinical course	160:174	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	4	2	theme	data	897:900	arg1	repository					866:875	a single, easily accessible repository	838:875	a single, easily accessible repository of cancer profiling data to aid-in	838:910	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
36776819	6	3	theme	molecular	1379:1387	arg1	patterns					1402:1409	molecular and clinical patterns	1379:1409	molecular and clinical patterns across cancer stages and subtypes	1379:1443	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	1	4	from	environment	322:332	arg1	influences					209:218	influences	209:218	influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment	209:332	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	4	5	theme	single	840:845	arg1	repository					866:875	a single, easily accessible repository	838:875	a single, easily accessible repository of cancer profiling data to aid-in	838:910	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
36776819	5	6	theme	choices	1273:1279	arg1	combination					1155:1165	a combination	1153:1165	a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1153:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	1	7	theme	patient	258:264	arg1	status					244:249	the general health status	225:249	the general health status of the patient	225:264	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	1	7	theme	patient	258:264	arg1	mutations					291:299	germline and neoplastic mutations	267:299	mutations	291:299	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	1	7	theme	patient	258:264	arg1	co-morbidities					302:315	co-morbidities	302:315	co-morbidities	302:315	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	1	7	theme	patient	258:264	arg1	environment					322:332	environment	322:332	environment	322:332	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	3	8	theme	demographic	699:709	arg1	points					731:736	clinical, demographic, and lifestyle data points	689:736	clinical, demographic, and lifestyle data points of individual cancer patients	689:766	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	1	9	from	co-morbidities	302:315	arg1	influences					209:218	influences	209:218	influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment	209:332	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	5	10	theme	compositions	1219:1230	arg1	combination					1155:1165	a combination	1153:1165	a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1153:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	4	11	with	researchers	821:831	arg1	repository					866:875	a single, easily accessible repository	838:875	a single, easily accessible repository of cancer profiling data to aid-in	838:910	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
36776819	5	12	theme	mutations	1178:1186	arg1	combination					1155:1165	a combination	1153:1165	a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1153:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	2	13	theme	individualized	360:373	arg1	treatment					375:383	an individualized treatment	357:383	an individualized treatment	357:383	To effectively tailor an individualized treatment to each patient, such multifactorial data must be presented to providers in an easy-to-access and easy-to-analyze fashion.
36776819	6	14	theme	integrated	1322:1331	arg1	database					1359:1366	an integrated, interactive, and growing database	1319:1366	an integrated, interactive, and growing database	1319:1366	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	1	15	theme	germline	267:274	arg1	mutations					291:299	germline and neoplastic mutations	267:299	mutations	291:299	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	1	16	from	mutations	291:299	arg1	influences					209:218	influences	209:218	influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment	209:332	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	5	17	theme	lifestyle	1263:1271	arg1	choices					1273:1279	lifestyle choices	1263:1279	lifestyle choices	1263:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	3	18	theme	relational	531:540	arg1	database					542:549	a relational database	529:549	a relational database	529:549	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	5	19	with	types	1081:1085	arg1	possibility					1096:1106	the possibility	1092:1106	the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1092:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	2	20	theme	such	402:405	arg1	data					422:425	such multifactorial data	402:425	such multifactorial data	402:425	To effectively tailor an individualized treatment to each patient, such multifactorial data must be presented to providers in an easy-to-access and easy-to-analyze fashion.
36776819	6	21	theme	growing	1351:1357	arg1	database					1359:1366	an integrated, interactive, and growing database	1319:1366	an integrated, interactive, and growing database	1319:1366	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	3	22	theme	lifestyle	716:724	arg1	points					731:736	clinical, demographic, and lifestyle data points	689:736	clinical, demographic, and lifestyle data points of individual cancer patients	689:766	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	1	23	theme	Nuanced	98:104	arg1	care					121:124	Nuanced cancer patient care	98:124	Nuanced cancer patient care	98:124	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	1	24	theme	cancer	179:184	arg1	development					144:154	development	144:154	development	144:154	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	1	24	theme	cancer	179:184	arg1	course					169:174	clinical course	160:174	clinical course	160:174	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	3	25	theme	data	726:729	arg1	points					731:736	clinical, demographic, and lifestyle data points	689:736	clinical, demographic, and lifestyle data points of individual cancer patients	689:766	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	6	26	theme	diagnostic	1486:1495	arg1	power					1512:1516	increased diagnostic and prognostic power	1476:1516	increased diagnostic and prognostic power	1476:1516	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	1	27	theme	cancer	106:111	arg1	care					121:124	Nuanced cancer patient care	98:124	Nuanced cancer patient care	98:124	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	4	28	theme	profiling	887:895	arg1	data					897:900	cancer profiling data	880:900	cancer profiling data	880:900	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
36776819	0	29	theme	Comprehensive	0:12	arg1	database					57:64	Comprehensive and user-analytics-friendly cancer patient database	0:64	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers	0:95	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers.
36776819	3	30	theme	patients	759:766	arg1	points					731:736	clinical, demographic, and lifestyle data points	689:736	clinical, demographic, and lifestyle data points of individual cancer patients	689:766	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	1	31	theme	patient	113:119	arg1	care					121:124	Nuanced cancer patient care	98:124	Nuanced cancer patient care	98:124	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	0	32	theme	cancer	42:47	arg1	database					57:64	Comprehensive and user-analytics-friendly cancer patient database	0:64	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers	0:95	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers.
36776819	1	33	theme	neoplastic	280:289	arg1	mutations					291:299	germline and neoplastic mutations	267:299	mutations	291:299	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	1	34	dep	development	144:154	arg1	the					140:142	the	140:142	the	140:142	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	6	35	theme	increased	1476:1484	arg1	power					1512:1516	increased diagnostic and prognostic power	1476:1516	increased diagnostic and prognostic power	1476:1516	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	0	36	theme	user-analytics-friendly	18:40	arg1	database					57:64	Comprehensive and user-analytics-friendly cancer patient database	0:64	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers	0:95	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers.
36776819	3	37	theme	individual	741:750	arg1	patients					759:766	individual cancer patients	741:766	individual cancer patients	741:766	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	3	38	theme	tumor	659:663	arg1	profiles					674:681	tumor glycomic profiles	659:681	tumor glycomic profiles	659:681	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	5	39	theme	molecular	1120:1128	arg1	signatures					1130:1139	"molecular signatures	1119:1139	"molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1119:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	5	40	from	groups	1030:1035	arg1	cohorts					1011:1017	cohorts	1011:1017	cohorts from these groups	1011:1035	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	5	41	theme	care	982:985	arg1	providers					987:995	care providers	982:995	care providers	982:995	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	2	42	theme	easy-to-analyze	483:497	arg1	fashion					499:505	an easy-to-access and easy-to-analyze fashion	461:505	an easy-to-access and easy-to-analyze fashion	461:505	To effectively tailor an individualized treatment to each patient, such multifactorial data must be presented to providers in an easy-to-access and easy-to-analyze fashion.
36776819	3	43	theme	cancer	752:757	arg1	patients					759:766	individual cancer patients	741:766	individual cancer patients	741:766	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	6	44	theme	interactive	1334:1344	arg1	database					1359:1366	an integrated, interactive, and growing database	1319:1366	an integrated, interactive, and growing database	1319:1366	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	3	45	theme	glycomic	665:672	arg1	profiles					674:681	tumor glycomic profiles	659:681	tumor glycomic profiles	659:681	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	3	46	theme	mutations	613:621	arg1	status					582:587	status	582:587	status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles	582:681	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	5	47	theme	glycan	1212:1217	arg1	compositions					1219:1230	glycan compositions	1212:1230	glycan compositions	1212:1230	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	0	48	theme	patient	49:55	arg1	database					57:64	Comprehensive and user-analytics-friendly cancer patient database	0:64	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers	0:95	Comprehensive and user-analytics-friendly cancer patient database for physicians and researchers.
36776819	6	49	theme	clinical	1393:1400	arg1	patterns					1402:1409	molecular and clinical patterns	1379:1409	molecular and clinical patterns across cancer stages and subtypes	1379:1443	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	4	50	theme	individualized	924:937	arg1	treatment					939:947	individualized treatment	924:947	individualized treatment	924:947	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
36776819	3	51	theme	serum	649:653	arg1	status					582:587	status	582:587	status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles	582:681	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	4	52	dep	single	840:845	arg1	accessible					855:864	accessible	855:864	accessible	855:864	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
36776819	5	53	theme	levels	1204:1209	arg1	combination					1155:1165	a combination	1153:1165	a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1153:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	2	54	theme	multifactorial	407:420	arg1	data					422:425	such multifactorial data	402:425	such multifactorial data	402:425	To effectively tailor an individualized treatment to each patient, such multifactorial data must be presented to providers in an easy-to-access and easy-to-analyze fashion.
36776819	2	55	theme	easy-to-access	464:477	arg1	fashion					499:505	an easy-to-access and easy-to-analyze fashion	461:505	an easy-to-access and easy-to-analyze fashion	461:505	To effectively tailor an individualized treatment to each patient, such multifactorial data must be presented to providers in an easy-to-access and easy-to-analyze fashion.
36776819	1	56	from	status	244:249	arg1	influences					209:218	influences	209:218	influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment	209:332	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	5	57	theme	galectin	1189:1196	arg1	levels					1204:1209	galectin serum levels	1189:1209	galectin serum levels	1189:1209	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	3	58	theme	cancer-critical	592:606	arg1	mutations					613:621	cancer-critical gene mutations	592:621	cancer-critical gene mutations	592:621	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	5	59	theme	data	1254:1257	arg1	combination					1155:1165	a combination	1153:1165	a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1153:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	5	60	theme	genetic	1170:1176	arg1	mutations					1178:1186	genetic mutations	1170:1186	genetic mutations	1170:1186	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	1	61	with	multifactorial	189:202	arg1	influences					209:218	influences	209:218	influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment	209:332	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	3	62	theme	gene	608:611	arg1	mutations					613:621	cancer-critical gene mutations	592:621	cancer-critical gene mutations	592:621	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	4	63	theme	cancer	880:885	arg1	data					897:900	cancer profiling data	880:900	cancer profiling data	880:900	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
36776819	5	64	theme	patient	1237:1243	arg1	data					1254:1257	patient clinical data	1237:1257	patient clinical data	1237:1257	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	3	65	theme	clinical	689:696	arg1	points					731:736	clinical, demographic, and lifestyle data points	689:736	clinical, demographic, and lifestyle data points of individual cancer patients	689:766	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	3	66	theme	profiles	639:646	arg1	status					582:587	status	582:587	status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles	582:681	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	5	67	theme	interactive	954:964	arg1	database					966:973	Our interactive database	950:973	Our interactive database	950:973	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	1	68	theme	general	229:235	arg1	status					244:249	the general health status	225:249	the general health status of the patient	225:264	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	5	69	theme	different	1066:1074	arg1	types					1081:1085	different data types	1066:1085	different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1066:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	3	70	theme	profiles	674:681	arg1	status					582:587	status	582:587	status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles	582:681	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	5	71	theme	data	1076:1079	arg1	types					1081:1085	different data types	1066:1085	different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices	1066:1279	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	1	72	theme	health	237:242	arg1	status					244:249	the general health status	225:249	the general health status of the patient	225:264	Nuanced cancer patient care is needed, as the development and clinical course of cancer is multifactorial with influences from the general health status of the patient, germline and neoplastic mutations, co-morbidities, and environment.
36776819	3	73	theme	serum	624:628	arg1	profiles					639:646	serum galectin profiles	624:646	serum galectin profiles	624:646	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	6	74	theme	prognostic	1501:1510	arg1	power					1512:1516	increased diagnostic and prognostic power	1476:1516	increased diagnostic and prognostic power	1476:1516	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	5	75	theme	clinical	1245:1252	arg1	data					1254:1257	patient clinical data	1237:1257	patient clinical data	1237:1257	Our interactive database allows care providers to amalgamate cohorts from these groups to find correlations between different data types with the possibility of finding "molecular signatures" based upon a combination of genetic mutations, galectin serum levels, glycan compositions, and patient clinical data and lifestyle choices.
36776819	6	76	theme	cancer	1418:1423	arg1	stages					1425:1430	cancer stages	1418:1430	cancer stages	1418:1430	Our project provides a framework for an integrated, interactive, and growing database to analyze molecular and clinical patterns across cancer stages and subtypes and provides opportunities for increased diagnostic and prognostic power.
36776819	3	77	theme	galectin	630:637	arg1	profiles					639:646	serum galectin profiles	624:646	serum galectin profiles	624:646	To address the need, a relational database has been developed integrating status of cancer-critical gene mutations, serum galectin profiles, serum and tumor glycomic profiles, with clinical, demographic, and lifestyle data points of individual cancer patients.
36776819	4	78	with	physicians	806:815	arg1	repository					866:875	a single, easily accessible repository	838:875	a single, easily accessible repository of cancer profiling data to aid-in	838:910	The database, as a backend, provides physicians and researchers with a single, easily accessible repository of cancer profiling data to aid-in and enhance individualized treatment.
37269808	0	0	theme	SCFA-producing	111:124	arg1	bacteria					126:133	SCFA-producing bacteria	111:133	SCFA-producing bacteria	111:133	Zearalenone attenuates colitis associated colorectal tumorigenesis through Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion.
37269808	2	1	contain	has	335:337	arg2	effects					353:359	fewer adverse effects	339:359	fewer adverse effects	339:359	There is a need for a therapy that has fewer adverse effects and greater efficiency.
37269808	2	1	contain	has	335:337	arg1	therapy					322:328	a therapy	320:328	a therapy that has fewer adverse effects and greater efficiency	320:382	There is a need for a therapy that has fewer adverse effects and greater efficiency.
37269808	7	2	theme	Ki67	1084:1087	arg1	expression					1048:1057	the expression	1044:1057	the expression of proliferative marker, Ki67 and cyclin D1	1044:1101	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	5	3	from	CRC	667:669	arg1	model					755:759	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	3	4	theme	higher	503:508	arg1	doses					510:514	higher doses	503:514	higher doses	503:514	Zearalenone (ZEA), an estrogenic mycotoxin, has been demonstrated to exert apoptotic properties when administrated in higher doses.
37269808	7	5	theme	cleaved	1008:1014	arg1	caspase					1016:1022	parker, cleaved caspase 3	1000:1024	caspase	1016:1022	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	11	6	theme	colorectal	1619:1628	arg1	tumorigenesis					1630:1642	colorectal tumorigenesis	1619:1642	colorectal tumorigenesis	1619:1642	Overall, ZEA demonstrated a promising inhibitory effect on colorectal tumorigenesis and exhibited the potential for further development as a CRC treatment.
37269808	9	7	theme	faecal	1412:1417	arg1	content					1427:1433	the faecal acetate content	1408:1433	the faecal acetate content	1408:1433	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	8	8	theme	lower	1176:1180	arg1	vulnerability					1182:1194	lower vulnerability	1176:1194	lower vulnerability	1176:1194	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	10	9	from	decrease	1534:1541	arg1	count					1553:1557	tumour count	1546:1557	tumour count	1546:1557	Notably, unidentified Ruminococcaceae and Parabacteroidies were substantially correlated with the decrease in tumour count.
37269808	5	10	from	mechanisms	690:699	arg1	model					755:759	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	8	11	theme	microbiota	1112:1121	arg1	composition					1123:1133	The gut microbiota composition	1104:1133	The gut microbiota composition in ZEA group	1104:1146	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	6	12	theme	crypt	865:869	arg1	depth					871:875	colonic crypt depth	857:875	colonic crypt depth	857:875	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	7	13	theme	marker	1076:1081	arg1	expression					1048:1057	the expression	1044:1057	the expression of proliferative marker, Ki67 and cyclin D1	1044:1101	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	7	14	theme	Ras/Raf/ERK/cyclin	930:947	arg1	D1					949:950	Ras/Raf/ERK/cyclin D1	930:950	Ras/Raf/ERK/cyclin D1 pathway	930:958	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	11	15	theme	inhibitory	1598:1607	arg1	effect					1609:1614	a promising inhibitory effect	1586:1614	a promising inhibitory effect on colorectal tumorigenesis	1586:1642	Overall, ZEA demonstrated a promising inhibitory effect on colorectal tumorigenesis and exhibited the potential for further development as a CRC treatment.
37269808	1	16	theme	healthcare	278:287	arg1	providers					289:297	healthcare providers	278:297	healthcare providers	278:297	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	8	17	theme	gut	1108:1110	arg1	composition					1123:1133	The gut microbiota composition	1104:1133	The gut microbiota composition in ZEA group	1104:1146	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	6	18	theme	colonic	857:863	arg1	depth					871:875	colonic crypt depth	857:875	colonic crypt depth	857:875	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	4	19	from	valid	578:582	arg1	setting					598:604	an in vivo setting	587:604	an in vivo setting	587:604	However, it is unclear whether such apoptotic effect remains valid in an in vivo setting.
37269808	2	20	theme	adverse	345:351	arg1	effects					353:359	fewer adverse effects	339:359	fewer adverse effects	339:359	There is a need for a therapy that has fewer adverse effects and greater efficiency.
37269808	11	21	theme	further	1676:1682	arg1	development					1684:1694	further development	1676:1694	further development	1676:1694	Overall, ZEA demonstrated a promising inhibitory effect on colorectal tumorigenesis and exhibited the potential for further development as a CRC treatment.
37269808	2	22	dep	efficiency	373:382	arg1	greater					365:371	greater	365:371	greater	365:371	There is a need for a therapy that has fewer adverse effects and greater efficiency.
37269808	11	23	from	effect	1609:1614	arg1	tumorigenesis					1630:1642	colorectal tumorigenesis	1619:1642	colorectal tumorigenesis	1619:1642	Overall, ZEA demonstrated a promising inhibitory effect on colorectal tumorigenesis and exhibited the potential for further development as a CRC treatment.
37269808	5	24	theme	dextran	722:728	arg1	sulfate					737:743	azoxymethane/ dextran sodium sulfate	708:743	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	5	24	theme	dextran	722:728	arg1	AOM/DSS					746:752	AOM/DSS	746:752	AOM/DSS	746:752	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	5	25	theme	current	611:617	arg1	study					619:623	The current study	607:623	The current study	607:623	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	4	26	theme	in	590:591	arg1	setting					598:604	an in vivo setting	587:604	an in vivo setting	587:604	However, it is unclear whether such apoptotic effect remains valid in an in vivo setting.
37269808	5	27	theme	azoxymethane/	708:720	arg1	sulfate					737:743	azoxymethane/ dextran sodium sulfate	708:743	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	5	27	theme	azoxymethane/	708:720	arg1	AOM/DSS					746:752	AOM/DSS	746:752	AOM/DSS	746:752	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	9	28	dep	bacteria	1328:1335	arg1	Blautia					1388:1394	Blautia	1388:1394	Blautia	1388:1394	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	28	dep	bacteria	1328:1335	arg1	Parabacteroidies					1367:1382	Parabacteroidies	1367:1382	Parabacteroidies	1367:1382	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	28	dep	bacteria	1328:1335	arg1	bacteria					1328:1335	bacteria	1328:1335	bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia	1328:1394	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	6	29	theme	colon	843:847	arg1	weight					849:854	colon weight	843:854	colon weight	843:854	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	9	30	theme	chain	1292:1296	arg1	acids					1304:1308	short chain fatty acids	1286:1308	short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content	1286:1433	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	30	theme	chain	1292:1296	arg1	SCFAs					1311:1315	SCFAs	1311:1315	SCFAs	1311:1315	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	7	31	theme	caspase	1016:1022	arg1	expression					976:985	the expression	972:985	the expression of apoptosis parker, cleaved caspase 3	972:1024	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	1	32	theme	leading	201:207	arg1	death					209:213	its leading death	197:213	its leading death causing rate	197:226	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	8	33	theme	ZEA	1138:1140	arg1	group					1142:1146	ZEA group	1138:1146	ZEA group	1138:1146	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	4	34	theme	such	548:551	arg1	effect					563:568	such apoptotic effect	548:568	such apoptotic effect	548:568	However, it is unclear whether such apoptotic effect remains valid in an in vivo setting.
37269808	1	35	theme	death	209:213	arg1	prevalence					155:164	The high prevalence	146:164	The high prevalence of colorectal cancer (CRC) and its leading death causing rate	146:226	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	8	36	theme	higher	1155:1160	arg1	stability					1162:1170	higher stability	1155:1170	higher stability	1155:1170	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	9	37	theme	fatty	1298:1302	arg1	acids					1304:1308	short chain fatty acids	1286:1308	short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content	1286:1433	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	37	theme	fatty	1298:1302	arg1	SCFAs					1311:1315	SCFAs	1311:1315	SCFAs	1311:1315	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	38	theme	acetate	1419:1425	arg1	content					1427:1433	the faecal acetate content	1408:1433	the faecal acetate content	1408:1433	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	39	theme	acids	1304:1308	arg1	abundance					1273:1281	the abundance	1269:1281	the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content	1269:1433	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	0	40	dep	pathway	87:93	arg1	promotion					135:143	promotion	135:143	promotion	135:143	Zearalenone attenuates colitis associated colorectal tumorigenesis through Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion.
37269808	0	40	dep	pathway	87:93	arg1	suppression					95:105	suppression	95:105	suppression	95:105	Zearalenone attenuates colitis associated colorectal tumorigenesis through Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion.
37269808	3	41	theme	apoptotic	460:468	arg1	properties					470:479	apoptotic properties	460:479	apoptotic properties	460:479	Zearalenone (ZEA), an estrogenic mycotoxin, has been demonstrated to exert apoptotic properties when administrated in higher doses.
37269808	4	42	theme	apoptotic	553:561	arg1	effect					563:568	such apoptotic effect	548:568	such apoptotic effect	548:568	However, it is unclear whether such apoptotic effect remains valid in an in vivo setting.
37269808	5	43	theme	underlying	679:688	arg1	mechanisms					690:699	its underlying mechanisms	675:699	its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	675:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	6	44	theme	tumours	834:840	arg1	depth					871:875	colonic crypt depth	857:875	colonic crypt depth	857:875	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	6	44	theme	tumours	834:840	arg1	fibrosis					887:894	collagen fibrosis	878:894	collagen fibrosis	878:894	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	6	44	theme	tumours	834:840	arg1	weight					907:912	spleen weight	900:912	spleen weight	900:912	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	6	44	theme	tumours	834:840	arg1	weight					849:854	colon weight	843:854	colon weight	843:854	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	6	44	theme	tumours	834:840	arg1	number					824:829	the total number	814:829	the total number of tumours	814:840	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	11	45	theme	CRC	1701:1703	arg1	treatment					1705:1713	a CRC treatment	1699:1713	a CRC treatment	1699:1713	Overall, ZEA demonstrated a promising inhibitory effect on colorectal tumorigenesis and exhibited the potential for further development as a CRC treatment.
37269808	8	46	theme	microbial	1203:1211	arg1	community					1213:1221	the microbial community	1199:1221	the microbial community	1199:1221	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	7	47	theme	cyclin	1093:1098	arg1	D1					1100:1101	cyclin D1	1093:1101	cyclin D1	1093:1101	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	3	48	theme	estrogenic	407:416	arg1	mycotoxin					418:426	an estrogenic mycotoxin	404:426	an estrogenic mycotoxin	404:426	Zearalenone (ZEA), an estrogenic mycotoxin, has been demonstrated to exert apoptotic properties when administrated in higher doses.
37269808	3	48	theme	estrogenic	407:416	arg1	Zearalenone					385:395	Zearalenone	385:395	Zearalenone (ZEA)	385:401	Zearalenone (ZEA), an estrogenic mycotoxin, has been demonstrated to exert apoptotic properties when administrated in higher doses.
37269808	0	49	theme	colorectal	42:51	arg1	tumorigenesis					53:65	colorectal tumorigenesis	42:65	colorectal tumorigenesis	42:65	Zearalenone attenuates colitis associated colorectal tumorigenesis through Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion.
37269808	8	50	theme	AOM/DSS	1240:1246	arg1	group					1248:1252	AOM/DSS group	1240:1252	AOM/DSS group	1240:1252	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	10	51	theme	tumour	1546:1551	arg1	count					1553:1557	tumour count	1546:1557	tumour count	1546:1557	Notably, unidentified Ruminococcaceae and Parabacteroidies were substantially correlated with the decrease in tumour count.
37269808	0	52	theme	Ras/Raf/ERK	75:85	arg1	pathway					87:93	Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion	75:143	pathway	87:93	Zearalenone attenuates colitis associated colorectal tumorigenesis through Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion.
37269808	5	53	from	effect	650:655	arg1	CRC					667:669	CRC	667:669	CRC	667:669	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	5	53	from	effect	650:655	arg1	mechanisms					690:699	its underlying mechanisms	675:699	its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	675:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	1	54	from	burden	255:260	arg1	patients					265:272	patients	265:272	patients	265:272	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	1	54	from	burden	255:260	arg1	providers					289:297	healthcare providers	278:297	healthcare providers	278:297	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	5	55	theme	sulfate	737:743	arg1	model					755:759	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	0	56	dep	tumorigenesis	53:65	arg1	pathway					87:93	Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion	75:143	pathway	87:93	Zearalenone attenuates colitis associated colorectal tumorigenesis through Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion.
37269808	0	56	dep	tumorigenesis	53:65	arg1	bacteria					126:133	SCFA-producing bacteria	111:133	SCFA-producing bacteria	111:133	Zearalenone attenuates colitis associated colorectal tumorigenesis through Ras/Raf/ERK pathway suppression and SCFA-producing bacteria promotion.
37269808	7	57	theme	apoptosis	990:998	arg1	caspase					1016:1022	parker, cleaved caspase 3	1000:1024	caspase	1016:1022	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	1	58	theme	high	150:153	arg1	prevalence					155:164	The high prevalence	146:164	The high prevalence of colorectal cancer (CRC) and its leading death causing rate	146:226	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	7	59	theme	D1	1100:1101	arg1	expression					1048:1057	the expression	1044:1057	the expression of proliferative marker, Ki67 and cyclin D1	1044:1101	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	5	60	theme	ZEA	660:662	arg1	effect					650:655	the effect	646:655	the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	646:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	6	61	theme	total	818:822	arg1	number					824:829	the total number	814:829	the total number of tumours	814:840	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	7	62	theme	proliferative	1062:1074	arg1	marker					1076:1081	proliferative marker	1062:1081	proliferative marker	1062:1081	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	2	63	theme	fewer	339:343	arg1	effects					353:359	fewer adverse effects	339:359	fewer adverse effects	339:359	There is a need for a therapy that has fewer adverse effects and greater efficiency.
37269808	7	64	theme	parker	1000:1005	arg1	caspase					1016:1022	parker, cleaved caspase 3	1000:1024	caspase	1016:1022	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	8	65	from	composition	1123:1133	arg1	group					1142:1146	ZEA group	1138:1146	ZEA group	1138:1146	The gut microbiota composition in ZEA group showed higher stability and lower vulnerability in the microbial community when compared to AOM/DSS group.
37269808	11	66	theme	promising	1588:1596	arg1	effect					1609:1614	a promising inhibitory effect	1586:1614	a promising inhibitory effect on colorectal tumorigenesis	1586:1642	Overall, ZEA demonstrated a promising inhibitory effect on colorectal tumorigenesis and exhibited the potential for further development as a CRC treatment.
37269808	9	67	theme	unidentified	1337:1348	arg1	Blautia					1388:1394	Blautia	1388:1394	Blautia	1388:1394	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	67	theme	unidentified	1337:1348	arg1	Parabacteroidies					1367:1382	Parabacteroidies	1367:1382	Parabacteroidies	1367:1382	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	67	theme	unidentified	1337:1348	arg1	bacteria					1328:1335	bacteria	1328:1335	bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia	1328:1394	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	1	68	theme	colorectal	169:178	arg1	CRC					188:190	CRC	188:190	CRC	188:190	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	1	68	theme	colorectal	169:178	arg1	cancer					180:185	colorectal cancer	169:185	colorectal cancer (CRC)	169:191	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	1	69	theme	considerable	242:253	arg1	burden					255:260	a considerable burden	240:260	a considerable burden on patients and healthcare providers	240:297	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	5	70	theme	sodium	730:735	arg1	sulfate					737:743	azoxymethane/ dextran sodium sulfate	708:743	the azoxymethane/ dextran sodium sulfate (AOM/DSS) model	704:759	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	5	70	theme	sodium	730:735	arg1	AOM/DSS					746:752	AOM/DSS	746:752	AOM/DSS	746:752	The current study aimed to investigate the effect of ZEA on CRC and its underlying mechanisms in the azoxymethane/ dextran sodium sulfate (AOM/DSS) model.
37269808	4	71	dep	in	590:591	arg1	vivo					593:596	vivo	593:596	vivo	593:596	However, it is unclear whether such apoptotic effect remains valid in an in vivo setting.
37269808	1	72	theme	cancer	180:185	arg1	prevalence					155:164	The high prevalence	146:164	The high prevalence of colorectal cancer (CRC) and its leading death causing rate	146:226	The high prevalence of colorectal cancer (CRC) and its leading death causing rate have placed a considerable burden on patients and healthcare providers.
37269808	4	73	from	setting	598:604	arg1	valid					578:582	valid	578:582	valid	578:582	However, it is unclear whether such apoptotic effect remains valid in an in vivo setting.
37269808	6	74	theme	collagen	878:885	arg1	fibrosis					887:894	collagen fibrosis	878:894	collagen fibrosis	878:894	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
37269808	7	75	theme	D1	949:950	arg1	pathway					952:958	Ras/Raf/ERK/cyclin D1 pathway	930:958	Ras/Raf/ERK/cyclin D1 pathway	930:958	ZEA suppressed Ras/Raf/ERK/cyclin D1 pathway, increasing the expression of apoptosis parker, cleaved caspase 3, while decreasing the expression of proliferative marker, Ki67 and cyclin D1.
37269808	9	76	theme	short	1286:1290	arg1	acids					1304:1308	short chain fatty acids	1286:1308	short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content	1286:1433	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	9	76	theme	short	1286:1290	arg1	SCFAs					1311:1315	SCFAs	1311:1315	SCFAs	1311:1315	ZEA increased the abundance of short chain fatty acids (SCFAs) producing bacteria unidentified Ruminococcaceae, Parabacteroidies and Blautia, as well as the faecal acetate content.
37269808	6	77	theme	spleen	900:905	arg1	weight					907:912	spleen weight	900:912	spleen weight	900:912	Our results revealed that ZEA significantly lowered the total number of tumours, colon weight, colonic crypt depth, collagen fibrosis and spleen weight.
35982037	4	0	theme	composition	896:906	arg1	modification					862:873	simultaneous modification	849:873	simultaneous modification of the gut bacterial composition	849:906	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	1	1	from	determinant	163:173	arg1	diseases					186:193	various diseases	178:193	various diseases	178:193	The gut microbiome is an important determinant in various diseases.
35982037	3	2	theme	anti-inflammatory	480:496	arg1	effects					498:504	anti-inflammatory effects	480:504	anti-inflammatory effects	480:504	Oral administration of B. wexlerae to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet-induced obesity and diabetes.
35982037	4	3	theme	bacterial	886:894	arg1	composition					896:906	the gut bacterial composition	878:906	the gut bacterial composition	878:906	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	5	4	theme	unique	931:936	arg1	pathways					949:956	unique regulatory pathways	931:956	unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders	931:1088	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	5	5	theme	regulatory	938:947	arg1	pathways					949:956	unique regulatory pathways	931:956	unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders	931:1088	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	4	6	theme	beneficial	573:582	arg1	effects					584:590	The beneficial effects	569:590	The beneficial effects of B. wexlerae	569:605	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	3	7	theme	diet-induced	534:545	arg1	obesity					547:553	high-fat diet-induced obesity	525:553	high-fat diet-induced obesity	525:553	Oral administration of B. wexlerae to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet-induced obesity and diabetes.
35982037	0	8	theme	metabolic	84:92	arg1	remodeling					94:103	metabolic remodeling	84:103	metabolic remodeling of the gut microbiota	84:125	Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota.
35982037	5	9	theme	novel	1008:1012	arg1	strategies					1014:1023	novel strategies	1008:1023	novel strategies in preventive and therapeutic approaches for metabolic disorders	1008:1088	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	2	10	theme	Blautia	272:278	arg1	genus					280:284	the Blautia genus	268:284	the Blautia genus	268:284	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	2	10	theme	Blautia	272:278	arg1	bacterium					326:334	a commensal bacterium	314:334	a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus	314:405	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	4	11	theme	unique	627:632	arg1	metabolism					645:654	unique amino-acid metabolism	627:654	unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine	627:718	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	4	12	theme	succinate	811:819	arg1	production					797:806	production	797:806	production of succinate, lactate, and acetate	797:841	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	4	12	theme	succinate	811:819	arg1	accumulation					765:776	the accumulation	761:776	the accumulation of amylopectin	761:791	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	1	13	theme	important	153:161	arg1	microbiome					136:145	The gut microbiome	128:145	The gut microbiome	128:145	The gut microbiome is an important determinant in various diseases.
35982037	1	13	theme	important	153:161	arg1	determinant					163:173	an important determinant	150:173	an important determinant in various diseases	150:193	The gut microbiome is an important determinant in various diseases.
35982037	3	14	theme	high-fat	525:532	arg1	obesity					547:553	high-fat diet-induced obesity	525:553	high-fat diet-induced obesity	525:553	Oral administration of B. wexlerae to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet-induced obesity and diabetes.
35982037	5	15	theme	metabolic	1070:1078	arg1	disorders					1080:1088	metabolic disorders	1070:1088	metabolic disorders	1070:1088	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	4	16	theme	gut	882:884	arg1	composition					896:906	the gut bacterial composition	878:906	the gut bacterial composition	878:906	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	5	17	theme	microbial	970:978	arg1	metabolism					980:989	microbial metabolism	970:989	microbial metabolism	970:989	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	0	18	theme	Oral	0:3	arg1	administration					5:18	Oral administration	0:18	Oral administration of Blautia wexlerae	0:38	Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota.
35982037	5	19	theme	metabolism	980:989	arg1	pathways					949:956	unique regulatory pathways	931:956	unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders	931:1088	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	3	20	theme	Oral	408:411	arg1	administration					413:426	Oral administration	408:426	Oral administration of B. wexlerae to mice	408:449	Oral administration of B. wexlerae to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet-induced obesity and diabetes.
35982037	0	21	theme	Blautia	23:29	arg1	wexlerae					31:38	Blautia wexlerae	23:38	Blautia wexlerae	23:38	Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota.
35982037	5	22	theme	therapeutic	1043:1053	arg1	approaches					1055:1064	preventive and therapeutic approaches	1028:1064	preventive and therapeutic approaches	1028:1064	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	0	23	theme	microbiota	116:125	arg1	remodeling					94:103	metabolic remodeling	84:103	metabolic remodeling of the gut microbiota	84:125	Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota.
35982037	4	24	theme	amylopectin	781:791	arg1	production					797:806	production	797:806	production of succinate, lactate, and acetate	797:841	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	4	24	theme	amylopectin	781:791	arg1	accumulation					765:776	the accumulation	761:776	the accumulation of amylopectin	761:791	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	4	25	theme	amino-acid	634:643	arg1	metabolism					645:654	unique amino-acid metabolism	627:654	unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine	627:718	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	0	26	theme	gut	112:114	arg1	microbiota					116:125	the gut microbiota	108:125	the gut microbiota	108:125	Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota.
35982037	2	27	theme	diabetes	389:396	arg1	mellitus					398:405	type 2 diabetes mellitus	382:405	type 2 diabetes mellitus	382:405	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	1	28	theme	various	178:184	arg1	diseases					186:193	various diseases	178:193	various diseases	178:193	The gut microbiome is an important determinant in various diseases.
35982037	0	29	theme	wexlerae	31:38	arg1	administration					5:18	Oral administration	0:18	Oral administration of Blautia wexlerae	0:38	Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota.
35982037	2	30	theme	cross-sectional	214:228	arg1	study					230:234	a cross-sectional study	212:234	a cross-sectional study of Japanese adults	212:253	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	3	31	theme	wexlerae	434:441	arg1	administration					413:426	Oral administration	408:426	Oral administration of B. wexlerae to mice	408:449	Oral administration of B. wexlerae to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet-induced obesity and diabetes.
35982037	4	32	theme	simultaneous	849:860	arg1	modification					862:873	simultaneous modification	849:873	simultaneous modification of the gut bacterial composition	849:906	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	2	33	theme	commensal	316:324	arg1	genus					280:284	the Blautia genus	268:284	the Blautia genus	268:284	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	2	33	theme	commensal	316:324	arg1	bacterium					326:334	a commensal bacterium	314:334	a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus	314:405	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	4	34	theme	carbohydrate	724:735	arg1	metabolism					737:746	carbohydrate metabolism	724:746	carbohydrate metabolism	724:746	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	5	35	theme	preventive	1028:1037	arg1	approaches					1055:1064	preventive and therapeutic approaches	1028:1064	preventive and therapeutic approaches	1028:1064	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	4	36	theme	lactate	822:828	arg1	production					797:806	production	797:806	production of succinate, lactate, and acetate	797:841	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	4	36	theme	lactate	822:828	arg1	accumulation					765:776	the accumulation	761:776	the accumulation of amylopectin	761:791	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	4	37	theme	acetate	835:841	arg1	production					797:806	production	797:806	production of succinate, lactate, and acetate	797:841	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	4	37	theme	acetate	835:841	arg1	accumulation					765:776	the accumulation	761:776	the accumulation of amylopectin	761:791	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	5	38	theme	host	961:964	arg1	pathways					949:956	unique regulatory pathways	931:956	unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders	931:1088	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35982037	4	39	dep	metabolism	645:654	arg1	produce					659:665	produce	659:665	to produce S-adenosylmethionine, acetylcholine, and L-ornithine	656:718	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	2	40	theme	adults	248:253	arg1	study					230:234	a cross-sectional study	212:234	a cross-sectional study of Japanese adults	212:253	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	3	41	theme	metabolic	458:466	arg1	changes					468:474	metabolic changes	458:474	metabolic changes	458:474	Oral administration of B. wexlerae to mice induce metabolic changes and anti-inflammatory effects that decrease both high-fat diet-induced obesity and diabetes.
35982037	4	42	theme	wexlerae	598:605	arg1	effects					584:590	The beneficial effects	569:590	The beneficial effects of B. wexlerae	569:605	The beneficial effects of B. wexlerae are correlated with unique amino-acid metabolism to produce S-adenosylmethionine, acetylcholine, and L-ornithine and carbohydrate metabolism resulting in the accumulation of amylopectin and production of succinate, lactate, and acetate, with simultaneous modification of the gut bacterial composition.
35982037	2	43	theme	type	382:385	arg1	mellitus					398:405	type 2 diabetes mellitus	382:405	type 2 diabetes mellitus	382:405	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	2	44	theme	Japanese	239:246	arg1	adults					248:253	Japanese adults	239:253	Japanese adults	239:253	Here we perform a cross-sectional study of Japanese adults and identify the Blautia genus, especially B. wexlerae, as a commensal bacterium that is inversely correlated with obesity and type 2 diabetes mellitus.
35982037	1	45	theme	gut	132:134	arg1	microbiome					136:145	The gut microbiome	128:145	The gut microbiome	128:145	The gut microbiome is an important determinant in various diseases.
35982037	1	45	theme	gut	132:134	arg1	determinant					163:173	an important determinant	150:173	an important determinant in various diseases	150:193	The gut microbiome is an important determinant in various diseases.
35982037	0	46	theme	type	64:67	arg1	diabetes					71:78	type 2 diabetes	64:78	type 2 diabetes	64:78	Oral administration of Blautia wexlerae ameliorates obesity and type 2 diabetes via metabolic remodeling of the gut microbiota.
35982037	5	47	from	strategies	1014:1023	arg1	approaches					1055:1064	preventive and therapeutic approaches	1028:1064	preventive and therapeutic approaches	1028:1064	These findings reveal unique regulatory pathways of host and microbial metabolism that may provide novel strategies in preventive and therapeutic approaches for metabolic disorders.
35688918	6	0	theme	KBL697	885:890	arg1	Co-administration					853:869	Co-administration	853:869	Co-administration of L. gasseri KBL697 and IFX	853:898	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	7	1	theme	interleukin	1085:1095	arg1	IL					1098:1099	interleukin (IL)-2	1085:1102	interleukin (IL)-2	1085:1102	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	6	2	theme	L.	874:875	arg1	KBL697					885:890	L. gasseri KBL697	874:890	L. gasseri KBL697	874:890	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	4	3	theme	colony-forming	578:591	arg1	KBL697					615:620	2 × 109 colony-forming units/g of L. gasseri KBL697	570:620	2 × 109 colony-forming units/g of L. gasseri KBL697	570:620	2 × 109 colony-forming units/g of L. gasseri KBL697 were administered to seven-week-old female C57BL/6J mice daily by oral gavage.
35688918	10	4	theme	predicted	1551:1559	arg1	pathways					1594:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	3	5	theme	dextran	518:524	arg1	model					563:567	the dextran sodium sulfate-induced colitis mouse model	514:567	the dextran sodium sulfate-induced colitis mouse model	514:567	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	11	6	dep	L.	1708:1709	arg1	gasseri					1711:1717	gasseri	1711:1717	gasseri	1711:1717	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	10	7	theme	acid	1579:1582	arg1	pathways					1594:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	11	8	theme	prevention	1776:1785	arg1	effect					1698:1703	the synergistic effect	1682:1703	the synergistic effect of L. gasseri KBL697 and IFX co-administration	1682:1750	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	11	8	theme	prevention	1776:1785	arg1	method					1766:1771	a possible method	1755:1771	a possible method of prevention and treatment for IBD	1755:1807	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	3	9	theme	mouse	557:561	arg1	model					563:567	the dextran sodium sulfate-induced colitis mouse model	514:567	the dextran sodium sulfate-induced colitis mouse model	514:567	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	3	10	from	effects	351:357	arg1	model					563:567	the dextran sodium sulfate-induced colitis mouse model	514:567	the dextran sodium sulfate-induced colitis mouse model	514:567	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	3	11	theme	sulfate-induced	533:547	arg1	model					563:567	the dextran sodium sulfate-induced colitis mouse model	514:567	the dextran sodium sulfate-induced colitis mouse model	514:567	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	9	12	theme	altered	1438:1444	arg1	diversity					1462:1470	altered cecum microbial diversity	1438:1470	altered cecum microbial diversity	1438:1470	Furthermore, co-administered mice displayed altered cecum microbial diversity and composition with increases in the genus Prevotella.
35688918	4	13	theme	female	658:663	arg1	mice					674:677	seven-week-old female C57BL/6J mice	643:677	seven-week-old female C57BL/6J mice	643:677	2 × 109 colony-forming units/g of L. gasseri KBL697 were administered to seven-week-old female C57BL/6J mice daily by oral gavage.
35688918	9	14	theme	microbial	1452:1460	arg1	diversity					1462:1470	altered cecum microbial diversity	1438:1470	altered cecum microbial diversity	1438:1470	Furthermore, co-administered mice displayed altered cecum microbial diversity and composition with increases in the genus Prevotella.
35688918	6	15	theme	colitis	909:915	arg1	symptoms					917:924	colitis symptoms	909:924	colitis symptoms	909:924	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	8	16	theme	Expression	1216:1225	arg1	levels					1227:1232	Expression levels	1216:1232	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes	1216:1330	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	11	17	theme	treatment	1791:1799	arg1	effect					1698:1703	the synergistic effect	1682:1703	the synergistic effect of L. gasseri KBL697 and IFX co-administration	1682:1750	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	11	17	theme	treatment	1791:1799	arg1	method					1766:1771	a possible method	1755:1771	a possible method of prevention and treatment for IBD	1755:1807	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	1	18	theme	bowel	142:146	arg1	disease					148:154	Inflammatory bowel disease	129:154	Inflammatory bowel disease (IBD)	129:160	Inflammatory bowel disease (IBD) refers to disorders involving chronic inflammation of the gastrointestinal tract.
35688918	1	18	theme	bowel	142:146	arg1	IBD					157:159	IBD	157:159	IBD	157:159	Inflammatory bowel disease (IBD) refers to disorders involving chronic inflammation of the gastrointestinal tract.
35688918	6	19	theme	IFX	896:898	arg1	Co-administration					853:869	Co-administration	853:869	Co-administration of L. gasseri KBL697 and IFX	853:898	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	0	20	from	colitis	112:118	arg1	mice					123:126	mice	123:126	mice	123:126	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice.
35688918	8	21	theme	junction	1243:1250	arg1	genes					1252:1256	tight junction genes	1237:1256	tight junction genes	1237:1256	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	0	22	theme	factor-alpha	69:80	arg1	Co-administration					0:16	Co-administration	0:16	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab	0:101	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice.
35688918	6	23	theme	activity	966:973	arg1	index					975:979	disease activity index	958:979	disease activity index	958:979	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	9	24	theme	genus	1510:1514	arg1	Prevotella					1516:1525	the genus Prevotella	1506:1525	the genus Prevotella	1506:1525	Furthermore, co-administered mice displayed altered cecum microbial diversity and composition with increases in the genus Prevotella.
35688918	3	25	theme	co-administration	362:378	arg1	effects					351:357	the effects	347:357	the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model	347:567	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	8	26	theme	regulatory	1285:1294	arg1	cells					1296:1300	CD4 + CD25 + Foxp3 + T regulatory cells	1262:1300	CD4 + CD25 + Foxp3 + T regulatory cells	1262:1300	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	11	27	theme	co-administration	1734:1750	arg1	effect					1698:1703	the synergistic effect	1682:1703	the synergistic effect of L. gasseri KBL697 and IFX co-administration	1682:1750	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	11	27	theme	co-administration	1734:1750	arg1	method					1766:1771	a possible method	1755:1771	a possible method of prevention and treatment for IBD	1755:1807	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	6	28	theme	body	945:948	arg1	weight					950:955	body weight	945:955	body weight	945:955	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	3	29	theme	approved	457:464	arg1	-alpha					493:498	the first approved tumor necrosis factor (TNF)-alpha	447:498	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	10	30	theme	increased	1633:1641	arg1	level					1664:1668	increased acetate and butyrate level	1633:1668	increased acetate and butyrate level	1633:1668	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	8	31	theme	mesenteric	1309:1318	arg1	nodes					1326:1330	the mesenteric lymph nodes	1305:1330	the mesenteric lymph nodes	1305:1330	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	3	32	theme	infliximab	429:438	arg1	co-administration					362:378	co-administration	362:378	co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor,	362:509	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	3	33	theme	first	451:455	arg1	-alpha					493:498	the first approved tumor necrosis factor (TNF)-alpha	447:498	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	5	34	theme	IFX-treated	793:803	arg1	group					805:809	the IFX-treated group	789:809	the IFX-treated group	789:809	On day three, IFX (5 mg/kg) suspended in 1 × PBS (200 µL) was intravenously injected in the IFX-treated group and all mice were sacrificed on day nine.
35688918	3	35	theme	KBL697	418:423	arg1	co-administration					362:378	co-administration	362:378	co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor,	362:509	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	0	36	theme	KBL697	43:48	arg1	Co-administration					0:16	Co-administration	0:16	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab	0:101	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice.
35688918	10	37	from	changes	1536:1542	arg1	pathways					1594:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	3	38	theme	factor	481:486	arg1	-alpha					493:498	the first approved tumor necrosis factor (TNF)-alpha	447:498	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	0	39	theme	tumor	54:58	arg1	factor-alpha					69:80	tumor necrosis factor-alpha	54:80	tumor necrosis factor-alpha	54:80	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice.
35688918	0	40	dep	KBL697	43:48	arg1	infliximab					92:101	inhibitor infliximab	82:101	inhibitor infliximab	82:101	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice.
35688918	11	41	theme	L.	1708:1709	arg1	KBL697					1719:1724	L. gasseri KBL697	1708:1724	L. gasseri KBL697	1708:1724	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	8	42	theme	combined	1374:1381	arg1	treatment					1383:1391	the combined treatment	1370:1391	the combined treatment	1370:1391	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	3	43	theme	tumor	466:470	arg1	-alpha					493:498	the first approved tumor necrosis factor (TNF)-alpha	447:498	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	4	44	theme	oral	688:691	arg1	gavage					693:698	oral gavage	688:698	oral gavage	688:698	2 × 109 colony-forming units/g of L. gasseri KBL697 were administered to seven-week-old female C57BL/6J mice daily by oral gavage.
35688918	4	45	theme	units/g of L.	593:605	arg1	KBL697					615:620	2 × 109 colony-forming units/g of L. gasseri KBL697	570:620	2 × 109 colony-forming units/g of L. gasseri KBL697	570:620	2 × 109 colony-forming units/g of L. gasseri KBL697 were administered to seven-week-old female C57BL/6J mice daily by oral gavage.
35688918	3	46	theme	-alpha	493:498	arg1	KBL697					418:423	KBL697	418:423	KBL697	418:423	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	3	46	theme	-alpha	493:498	arg1	inhibitor					500:508	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	7	47	theme	anti-inflammatory	1173:1189	arg1	IL-10					1163:1167	IL-10	1163:1167	IL-10	1163:1167	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	7	47	theme	anti-inflammatory	1173:1189	arg1	cytokine					1191:1198	an anti-inflammatory cytokine	1170:1198	an anti-inflammatory cytokine	1170:1198	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	3	48	theme	TNF	489:491	arg1	-alpha					493:498	the first approved tumor necrosis factor (TNF)-alpha	447:498	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	6	49	dep	L.	874:875	arg1	gasseri					877:883	gasseri	877:883	gasseri	877:883	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	10	50	theme	amino	1561:1565	arg1	pathways					1594:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	9	51	from	increases	1493:1501	arg1	Prevotella					1516:1525	the genus Prevotella	1506:1525	the genus Prevotella	1506:1525	Furthermore, co-administered mice displayed altered cecum microbial diversity and composition with increases in the genus Prevotella.
35688918	9	52	theme	co-administered	1407:1421	arg1	mice					1423:1426	co-administered mice	1407:1426	co-administered mice	1407:1426	Furthermore, co-administered mice displayed altered cecum microbial diversity and composition with increases in the genus Prevotella.
35688918	4	53	theme	C57BL/6J	665:672	arg1	mice					674:677	seven-week-old female C57BL/6J mice	643:677	seven-week-old female C57BL/6J mice	643:677	2 × 109 colony-forming units/g of L. gasseri KBL697 were administered to seven-week-old female C57BL/6J mice daily by oral gavage.
35688918	10	54	theme	metabolic	1584:1592	arg1	pathways					1594:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	the predicted amino and nucleic acid metabolic pathways	1547:1601	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	7	55	theme	pro-inflammatory	1031:1046	arg1	TNF					1123:1125	TNF	1123:1125	TNF	1123:1125	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	7	55	theme	pro-inflammatory	1031:1046	arg1	interferon-gamma					1067:1082	interferon-gamma	1067:1082	interferon-gamma	1067:1082	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	7	55	theme	pro-inflammatory	1031:1046	arg1	IL-6					1105:1108	IL-6	1105:1108	IL-6	1105:1108	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	7	55	theme	pro-inflammatory	1031:1046	arg1	cytokines					1048:1056	pro-inflammatory cytokines	1031:1056	pro-inflammatory cytokines	1031:1056	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	7	55	theme	pro-inflammatory	1031:1046	arg1	IL-17A					1111:1116	IL-17A	1111:1116	IL-17A	1111:1116	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	7	55	theme	pro-inflammatory	1031:1046	arg1	IL					1098:1099	interleukin (IL)-2	1085:1102	interleukin (IL)-2	1085:1102	Additionally, pro-inflammatory cytokines, such as interferon-gamma, interleukin (IL)-2, IL-6, IL-17A, and TNF were significantly decreased, while IL-10, an anti-inflammatory cytokine, was increased.
35688918	3	56	theme	colitis	549:555	arg1	model					563:567	the dextran sodium sulfate-induced colitis mouse model	514:567	the dextran sodium sulfate-induced colitis mouse model	514:567	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	1	57	theme	chronic	192:198	arg1	inflammation					200:211	chronic inflammation	192:211	chronic inflammation of the gastrointestinal tract	192:241	Inflammatory bowel disease (IBD) refers to disorders involving chronic inflammation of the gastrointestinal tract.
35688918	10	58	theme	nucleic	1571:1577	arg1	acid					1579:1582	nucleic acid	1571:1582	nucleic acid	1571:1582	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	3	59	theme	sodium	526:531	arg1	model					563:567	the dextran sodium sulfate-induced colitis mouse model	514:567	the dextran sodium sulfate-induced colitis mouse model	514:567	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	9	60	theme	cecum	1446:1450	arg1	diversity					1462:1470	altered cecum microbial diversity	1438:1470	altered cecum microbial diversity	1438:1470	Furthermore, co-administered mice displayed altered cecum microbial diversity and composition with increases in the genus Prevotella.
35688918	4	61	theme	seven-week-old	643:656	arg1	mice					674:677	seven-week-old female C57BL/6J mice	643:677	seven-week-old female C57BL/6J mice	643:677	2 × 109 colony-forming units/g of L. gasseri KBL697 were administered to seven-week-old female C57BL/6J mice daily by oral gavage.
35688918	2	62	theme	Well-established	244:259	arg1	treatments					261:270	Well-established treatments	244:270	Well-established treatments for IBD	244:278	Well-established treatments for IBD have not yet to be suggested.
35688918	1	63	theme	Inflammatory	129:140	arg1	disease					148:154	Inflammatory bowel disease	129:154	Inflammatory bowel disease (IBD)	129:160	Inflammatory bowel disease (IBD) refers to disorders involving chronic inflammation of the gastrointestinal tract.
35688918	1	63	theme	Inflammatory	129:140	arg1	IBD					157:159	IBD	157:159	IBD	157:159	Inflammatory bowel disease (IBD) refers to disorders involving chronic inflammation of the gastrointestinal tract.
35688918	6	64	theme	colon	982:986	arg1	length					988:993	colon length	982:993	colon length	982:993	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	11	65	theme	IFX	1730:1732	arg1	co-administration					1734:1750	IFX co-administration	1730:1750	IFX co-administration	1730:1750	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	0	66	theme	inhibitor	82:90	arg1	infliximab					92:101	inhibitor infliximab	82:101	inhibitor infliximab	82:101	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice.
35688918	4	67	dep	units/g of L.	593:605	arg1	gasseri					607:613	gasseri	607:613	gasseri	607:613	2 × 109 colony-forming units/g of L. gasseri KBL697 were administered to seven-week-old female C57BL/6J mice daily by oral gavage.
35688918	8	68	theme	tight	1237:1241	arg1	genes					1252:1256	tight junction genes	1237:1256	tight junction genes	1237:1256	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	11	69	theme	KBL697	1719:1724	arg1	effect					1698:1703	the synergistic effect	1682:1703	the synergistic effect of L. gasseri KBL697 and IFX co-administration	1682:1750	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	11	69	theme	KBL697	1719:1724	arg1	method					1766:1771	a possible method	1755:1771	a possible method of prevention and treatment for IBD	1755:1807	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	6	70	theme	disease	958:964	arg1	index					975:979	disease activity index	958:979	disease activity index	958:979	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	3	71	dep	gasseri	397:403	arg1	gasseri					409:415	L. gasseri	406:415	L. gasseri	406:415	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	8	72	theme	genes	1252:1256	arg1	levels					1227:1232	Expression levels	1216:1232	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes	1216:1330	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	11	73	theme	possible	1757:1764	arg1	effect					1698:1703	the synergistic effect	1682:1703	the synergistic effect of L. gasseri KBL697 and IFX co-administration	1682:1750	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	11	73	theme	possible	1757:1764	arg1	method					1766:1771	a possible method	1755:1771	a possible method of prevention and treatment for IBD	1755:1807	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	8	74	from	levels	1227:1232	arg1	nodes					1326:1330	the mesenteric lymph nodes	1305:1330	the mesenteric lymph nodes	1305:1330	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	8	75	theme	CD4 + CD25 + Foxp3 + T	1262:1283	arg1	cells					1296:1300	CD4 + CD25 + Foxp3 + T regulatory cells	1262:1300	CD4 + CD25 + Foxp3 + T regulatory cells	1262:1300	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	8	76	theme	cells	1296:1300	arg1	levels					1227:1232	Expression levels	1216:1232	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes	1216:1330	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	6	77	theme	histology	1000:1008	arg1	score					1010:1014	histology score	1000:1014	histology score	1000:1014	Co-administration of L. gasseri KBL697 and IFX improved colitis symptoms in mice, including body weight, disease activity index, colon length, and histology score.
35688918	8	78	theme	lymph	1320:1324	arg1	nodes					1326:1330	the mesenteric lymph nodes	1305:1330	the mesenteric lymph nodes	1305:1330	Expression levels of tight junction genes and CD4 + CD25 + Foxp3 + T regulatory cells in the mesenteric lymph nodes were synergistically upregulated with the combined treatment.
35688918	10	79	theme	butyrate	1655:1662	arg1	level					1664:1668	increased acetate and butyrate level	1633:1668	increased acetate and butyrate level	1633:1668	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	1	80	theme	gastrointestinal	220:235	arg1	tract					237:241	the gastrointestinal tract	216:241	the gastrointestinal tract	216:241	Inflammatory bowel disease (IBD) refers to disorders involving chronic inflammation of the gastrointestinal tract.
35688918	10	81	theme	acetate	1643:1649	arg1	level					1664:1668	increased acetate and butyrate level	1633:1668	increased acetate and butyrate level	1633:1668	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35688918	0	82	theme	necrosis	60:67	arg1	factor-alpha					69:80	tumor necrosis factor-alpha	54:80	tumor necrosis factor-alpha	54:80	Co-administration of Lactobacillus gasseri KBL697 and tumor necrosis factor-alpha inhibitor infliximab improves colitis in mice.
35688918	11	83	theme	synergistic	1686:1696	arg1	effect					1698:1703	the synergistic effect	1682:1703	the synergistic effect of L. gasseri KBL697 and IFX co-administration	1682:1750	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	11	83	theme	synergistic	1686:1696	arg1	method					1766:1771	a possible method	1755:1771	a possible method of prevention and treatment for IBD	1755:1807	Therefore, the synergistic effect of L. gasseri KBL697 and IFX co-administration is a possible method of prevention and treatment for IBD.
35688918	3	84	theme	necrosis	472:479	arg1	-alpha					493:498	the first approved tumor necrosis factor (TNF)-alpha	447:498	the first approved tumor necrosis factor (TNF)-alpha inhibitor	447:508	To address this gap, we investigated the effects of co-administration of Lactobacillus gasseri (L. gasseri) KBL697 and infliximab (IFX), the first approved tumor necrosis factor (TNF)-alpha inhibitor, on the dextran sodium sulfate-induced colitis mouse model.
35688918	1	85	theme	tract	237:241	arg1	inflammation					200:211	chronic inflammation	192:211	chronic inflammation of the gastrointestinal tract	192:241	Inflammatory bowel disease (IBD) refers to disorders involving chronic inflammation of the gastrointestinal tract.
35688918	10	86	theme	Related	1528:1534	arg1	changes					1536:1542	Related changes	1528:1542	Related changes in the predicted amino and nucleic acid metabolic pathways	1528:1601	Related changes in the predicted amino and nucleic acid metabolic pathways were also evident, along with increased acetate and butyrate level.
35650335	0	0	theme	oxidase/ROS/p42/p44	91:109	arg1	Cyclooxygenase-2					126:141	NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2	85:141	NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2	85:141	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	9	1	theme	ROS	1335:1337	arg1	accumulation					1339:1350	NADPH oxidase-dependent ROS accumulation	1311:1350	NADPH oxidase-dependent ROS accumulation in preadipocytes	1311:1367	LPS activated p42/p44 MAPK via NADPH oxidase-dependent ROS accumulation in preadipocytes.
35650335	8	2	theme	oxygen	1074:1079	arg1	species					1081:1087	reactive oxygen species	1065:1087	reactive oxygen species (ROS)	1065:1093	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	8	2	theme	oxygen	1074:1079	arg1	ROS					1090:1092	ROS	1090:1092	ROS	1090:1092	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	0	3	theme	NADPH	85:89	arg1	Cyclooxygenase-2					126:141	NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2	85:141	NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2	85:141	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	8	4	theme	reactive	1065:1072	arg1	species					1081:1087	reactive oxygen species	1065:1087	reactive oxygen species (ROS)	1065:1093	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	8	4	theme	reactive	1065:1072	arg1	ROS					1090:1092	ROS	1090:1092	ROS	1090:1092	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	8	5	theme	MAPK	1262:1265	arg1	phosphorylation					1223:1237	the phosphorylation	1219:1237	the phosphorylation of p47phox and p42/p44 MAPK	1219:1265	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	1	6	theme	child	192:196	arg1	obesity					198:204	the child obesity	188:204	especially the child obesity	177:204	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	1	6	theme	child	192:196	arg1	problem					168:174	a world-wide problem	155:174	a world-wide problem	155:174	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	0	7	theme	Cyclooxygenase-2	126:141	arg1	Involvement					70:80	Involvement	70:80	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.	0:142	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	0	8	from	Participation	0:12	arg1	expansion					59:67	hyperplasic adipose expansion	39:67	hyperplasic adipose expansion	39:67	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	10	9	theme	MAPK	1427:1430	arg1	activation					1432:1441	p42/p44 MAPK activation	1419:1441	p42/p44 MAPK activation	1419:1441	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	9	10	from	accumulation	1339:1350	arg1	preadipocytes					1355:1367	preadipocytes	1355:1367	preadipocytes	1355:1367	LPS activated p42/p44 MAPK via NADPH oxidase-dependent ROS accumulation in preadipocytes.
35650335	4	11	theme	lipopolysaccharide	599:616	arg1	roles					590:594	the roles	586:594	the roles of lipopolysaccharide (LPS) on manipulating adipose tissue development	586:665	The changed composition of gut microbiota is associated with obesity, revealing the roles of lipopolysaccharide (LPS) on manipulating adipose tissue development.
35650335	0	12	theme	MAPK-dependent	111:124	arg1	Cyclooxygenase-2					126:141	NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2	85:141	NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2	85:141	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	8	13	theme	species	1081:1087	arg1	scavenger					1052:1060	the scavenger	1048:1060	the scavenger of reactive oxygen species (ROS)	1048:1093	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	11	14	theme	PEG2	1629:1632	arg1	receptors					1634:1642	PEG2 receptors	1629:1642	PEG2 receptors	1629:1642	Moreover, LPS-induced preadipocyte proliferation and adipogenesis were abolished by the inhibition of COX-2 or PEG2 receptors.
35650335	11	15	theme	preadipocyte	1540:1551	arg1	proliferation					1553:1565	LPS-induced preadipocyte proliferation	1528:1565	LPS-induced preadipocyte proliferation	1528:1565	Moreover, LPS-induced preadipocyte proliferation and adipogenesis were abolished by the inhibition of COX-2 or PEG2 receptors.
35650335	12	16	theme	MAPK-dependent	1783:1796	arg1	expression					1804:1813	NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression	1757:1813	NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression	1757:1813	Taken together, our results suggested that LPS enhanced the proliferation and adipogenesis of preadipocytes via NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression.
35650335	3	17	theme	fat	345:347	arg1	mass					349:352	fat mass	345:352	fat mass	345:352	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	6	18	theme	LPS-modulated	831:843	arg1	obesity					845:851	LPS-modulated obesity	831:851	LPS-modulated obesity	831:851	Nevertheless, the underlying mechanisms behind LPS-modulated obesity are yet clearly elucidated.
35650335	7	19	from	expression	921:930	arg1	preadipocytes					1002:1014	preadipocytes	1002:1014	preadipocytes	1002:1014	This study showed that LPS enhanced the expression of cyclooxygenase-2 (COX-2), an inflammatory regulator of obesity, in preadipocytes.
35650335	11	20	theme	LPS-induced	1528:1538	arg1	proliferation					1553:1565	LPS-induced preadipocyte proliferation	1528:1565	LPS-induced preadipocyte proliferation	1528:1565	Moreover, LPS-induced preadipocyte proliferation and adipogenesis were abolished by the inhibition of COX-2 or PEG2 receptors.
35650335	3	21	theme	mass	349:352	arg1	expansion					332:340	The expansion	328:340	The expansion of fat mass in child age	328:365	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	12	22	dep	proliferation	1705:1717	arg1	the					1701:1703	the	1701:1703	the	1701:1703	Taken together, our results suggested that LPS enhanced the proliferation and adipogenesis of preadipocytes via NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression.
35650335	1	23	with	problem	168:174	arg1	complication					216:227	the complication	212:227	the complication of various metabolic diseases	212:257	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	7	24	theme	inflammatory	964:975	arg1	regulator					977:985	an inflammatory regulator	961:985	an inflammatory regulator of obesity	961:996	This study showed that LPS enhanced the expression of cyclooxygenase-2 (COX-2), an inflammatory regulator of obesity, in preadipocytes.
35650335	7	24	theme	inflammatory	964:975	arg1	cyclooxygenase-2					935:950	cyclooxygenase-2	935:950	cyclooxygenase-2 (COX-2)	935:958	This study showed that LPS enhanced the expression of cyclooxygenase-2 (COX-2), an inflammatory regulator of obesity, in preadipocytes.
35650335	10	25	theme	intracellular	1383:1395	arg1	ROS					1397:1399	intracellular ROS	1383:1399	intracellular ROS	1383:1399	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	8	26	theme	gene	1180:1183	arg1	expression					1185:1194	LPS-stimulated gene expression	1165:1194	LPS-stimulated gene expression of COX-2	1165:1203	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	10	27	theme	preadipocyte	1490:1501	arg1	proliferation					1503:1515	preadipocyte proliferation	1490:1515	preadipocyte proliferation	1490:1515	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	12	28	theme	COX-2	1798:1802	arg1	expression					1804:1813	NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression	1757:1813	NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression	1757:1813	Taken together, our results suggested that LPS enhanced the proliferation and adipogenesis of preadipocytes via NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression.
35650335	3	29	theme	tissue	420:425	arg1	hypertrophy					397:407	hypertrophy	397:407	hypertrophy of adipose tissue	397:425	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	3	29	theme	tissue	420:425	arg1	hyperplasia					381:391	hyperplasia	381:391	hyperplasia	381:391	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	6	30	theme	underlying	802:811	arg1	mechanisms					813:822	the underlying mechanisms	798:822	the underlying mechanisms behind LPS-modulated obesity	798:851	Nevertheless, the underlying mechanisms behind LPS-modulated obesity are yet clearly elucidated.
35650335	5	31	theme	pro-inflammatory	730:745	arg1	LPS					689:691	LPS	689:691	LPS	689:691	Studies suggest that LPS enters the circulation and acts as a pro-inflammatory regulator to facilitate pathologies.
35650335	5	31	theme	pro-inflammatory	730:745	arg1	regulator					747:755	a pro-inflammatory regulator	728:755	a pro-inflammatory regulator to facilitate pathologies	728:781	Studies suggest that LPS enters the circulation and acts as a pro-inflammatory regulator to facilitate pathologies.
35650335	8	32	theme	p47phox	1242:1248	arg1	phosphorylation					1223:1237	the phosphorylation	1219:1237	the phosphorylation of p47phox and p42/p44 MAPK	1219:1265	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	7	33	theme	obesity	990:996	arg1	regulator					977:985	an inflammatory regulator	961:985	an inflammatory regulator of obesity	961:996	This study showed that LPS enhanced the expression of cyclooxygenase-2 (COX-2), an inflammatory regulator of obesity, in preadipocytes.
35650335	7	33	theme	obesity	990:996	arg1	cyclooxygenase-2					935:950	cyclooxygenase-2	935:950	cyclooxygenase-2 (COX-2)	935:958	This study showed that LPS enhanced the expression of cyclooxygenase-2 (COX-2), an inflammatory regulator of obesity, in preadipocytes.
35650335	8	34	theme	p42/p44	1254:1260	arg1	MAPK					1262:1265	p42/p44 MAPK	1254:1265	p42/p44 MAPK	1254:1265	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	10	35	theme	COX-2	1469:1473	arg1	expression					1475:1484	LPS-mediated COX-2 expression	1456:1484	LPS-mediated COX-2 expression	1456:1484	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	3	36	theme	proliferation	457:469	arg1	importance					443:452	the importance	439:452	the importance of proliferation and adipogenesis of preadipocytes	439:503	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	0	37	theme	lipopolysaccharide	17:34	arg1	Participation					0:12	Participation	0:12	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.	0:142	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	8	38	theme	COX-2	1199:1203	arg1	expression					1185:1194	LPS-stimulated gene expression	1165:1194	LPS-stimulated gene expression of COX-2	1165:1203	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	12	39	theme	preadipocytes	1739:1751	arg1	proliferation					1705:1717	proliferation	1705:1717	proliferation	1705:1717	Taken together, our results suggested that LPS enhanced the proliferation and adipogenesis of preadipocytes via NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression.
35650335	12	39	theme	preadipocytes	1739:1751	arg1	adipogenesis					1723:1734	adipogenesis	1723:1734	adipogenesis	1723:1734	Taken together, our results suggested that LPS enhanced the proliferation and adipogenesis of preadipocytes via NADPH oxidase/ROS/p42/p44 MAPK-dependent COX-2 expression.
35650335	0	40	theme	adipose	51:57	arg1	expansion					59:67	hyperplasic adipose expansion	39:67	hyperplasic adipose expansion	39:67	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	8	41	theme	oxidase	1122:1128	arg1	inhibitors					1102:1111	the inhibitors	1098:1111	the inhibitors of NADPH oxidase or p42/p44 MAPK	1098:1144	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	1	42	theme	various	232:238	arg1	diseases					250:257	various metabolic diseases	232:257	various metabolic diseases	232:257	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	0	43	theme	hyperplasic	39:49	arg1	expansion					59:67	hyperplasic adipose expansion	39:67	hyperplasic adipose expansion	39:67	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	4	44	theme	microbiota	537:546	arg1	composition					518:528	The changed composition	506:528	The changed composition of gut microbiota	506:546	The changed composition of gut microbiota is associated with obesity, revealing the roles of lipopolysaccharide (LPS) on manipulating adipose tissue development.
35650335	3	45	theme	adipose	412:418	arg1	tissue					420:425	adipose tissue	412:425	adipose tissue	412:425	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	2	46	theme	Child	260:264	arg1	early					294:298	early	294:298	early	294:298	Child obesity can be developed as early as the age between 2 and 6.
35650335	2	46	theme	Child	260:264	arg1	obesity					266:272	Child obesity	260:272	Child obesity	260:272	Child obesity can be developed as early as the age between 2 and 6.
35650335	1	47	theme	metabolic	240:248	arg1	diseases					250:257	various metabolic diseases	232:257	various metabolic diseases	232:257	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	4	48	theme	gut	533:535	arg1	microbiota					537:546	gut microbiota	533:546	gut microbiota	533:546	The changed composition of gut microbiota is associated with obesity, revealing the roles of lipopolysaccharide (LPS) on manipulating adipose tissue development.
35650335	8	49	theme	p42/p44	1133:1139	arg1	MAPK					1141:1144	p42/p44 MAPK	1133:1144	p42/p44 MAPK	1133:1144	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	3	50	theme	preadipocytes	491:503	arg1	adipogenesis					475:486	adipogenesis	475:486	adipogenesis	475:486	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	3	50	theme	preadipocytes	491:503	arg1	proliferation					457:469	proliferation	457:469	proliferation	457:469	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	8	51	theme	NADPH	1116:1120	arg1	oxidase					1122:1128	NADPH oxidase	1116:1128	NADPH oxidase	1116:1128	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	9	52	theme	oxidase-dependent	1317:1333	arg1	accumulation					1339:1350	NADPH oxidase-dependent ROS accumulation	1311:1350	NADPH oxidase-dependent ROS accumulation in preadipocytes	1311:1367	LPS activated p42/p44 MAPK via NADPH oxidase-dependent ROS accumulation in preadipocytes.
35650335	9	53	theme	p42/p44	1294:1300	arg1	MAPK					1302:1305	p42/p44 MAPK	1294:1305	p42/p44 MAPK	1294:1305	LPS activated p42/p44 MAPK via NADPH oxidase-dependent ROS accumulation in preadipocytes.
35650335	10	54	theme	ROS	1397:1399	arg1	attenuation					1404:1414	attenuation	1404:1414	attenuation of p42/p44 MAPK activation	1404:1441	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	10	54	theme	ROS	1397:1399	arg1	Reduction					1370:1378	Reduction	1370:1378	Reduction of intracellular ROS	1370:1399	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	10	55	theme	LPS-mediated	1456:1467	arg1	expression					1475:1484	LPS-mediated COX-2 expression	1456:1484	LPS-mediated COX-2 expression	1456:1484	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	1	56	theme	world-wide	157:166	arg1	obesity					198:204	the child obesity	188:204	especially the child obesity	177:204	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	1	56	theme	world-wide	157:166	arg1	problem					168:174	a world-wide problem	155:174	a world-wide problem	155:174	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	1	56	theme	world-wide	157:166	arg1	Obesity					144:150	Obesity	144:150	Obesity	144:150	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	1	57	theme	diseases	250:257	arg1	complication					216:227	the complication	212:227	the complication of various metabolic diseases	212:257	Obesity is a world-wide problem, especially the child obesity, with the complication of various metabolic diseases.
35650335	8	58	theme	MAPK	1141:1144	arg1	inhibitors					1102:1111	the inhibitors	1098:1111	the inhibitors of NADPH oxidase or p42/p44 MAPK	1098:1144	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	3	59	from	expansion	332:340	arg1	age					363:365	child age	357:365	child age	357:365	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	10	60	theme	activation	1432:1441	arg1	attenuation					1404:1414	attenuation	1404:1414	attenuation of p42/p44 MAPK activation	1404:1441	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	10	60	theme	activation	1432:1441	arg1	Reduction					1370:1378	Reduction	1370:1378	Reduction of intracellular ROS	1370:1399	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	7	61	theme	cyclooxygenase-2	935:950	arg1	expression					921:930	the expression	917:930	the expression of cyclooxygenase-2 (COX-2), an inflammatory regulator of obesity, in preadipocytes	917:1014	This study showed that LPS enhanced the expression of cyclooxygenase-2 (COX-2), an inflammatory regulator of obesity, in preadipocytes.
35650335	3	62	theme	adipogenesis	475:486	arg1	importance					443:452	the importance	439:452	the importance of proliferation and adipogenesis of preadipocytes	439:503	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35650335	8	63	theme	LPS-stimulated	1165:1178	arg1	expression					1185:1194	LPS-stimulated gene expression	1165:1194	LPS-stimulated gene expression of COX-2	1165:1203	Pretreating preadipocytes with the scavenger of reactive oxygen species (ROS) or the inhibitors of NADPH oxidase or p42/p44 MAPK markedly decreased LPS-stimulated gene expression of COX-2 together with the phosphorylation of p47phox and p42/p44 MAPK, separately.
35650335	4	64	theme	tissue	648:653	arg1	development					655:665	adipose tissue development	640:665	adipose tissue development	640:665	The changed composition of gut microbiota is associated with obesity, revealing the roles of lipopolysaccharide (LPS) on manipulating adipose tissue development.
35650335	4	65	theme	adipose	640:646	arg1	development					655:665	adipose tissue development	640:665	adipose tissue development	640:665	The changed composition of gut microbiota is associated with obesity, revealing the roles of lipopolysaccharide (LPS) on manipulating adipose tissue development.
35650335	11	66	theme	receptors	1634:1642	arg1	inhibition					1606:1615	the inhibition	1602:1615	the inhibition of COX-2 or PEG2 receptors	1602:1642	Moreover, LPS-induced preadipocyte proliferation and adipogenesis were abolished by the inhibition of COX-2 or PEG2 receptors.
35650335	4	67	theme	changed	510:516	arg1	composition					518:528	The changed composition	506:528	The changed composition of gut microbiota	506:546	The changed composition of gut microbiota is associated with obesity, revealing the roles of lipopolysaccharide (LPS) on manipulating adipose tissue development.
35650335	0	68	dep	Participation	0:12	arg1	Involvement					70:80	Involvement	70:80	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.	0:142	Participation of lipopolysaccharide in hyperplasic adipose expansion: Involvement of NADPH oxidase/ROS/p42/p44 MAPK-dependent Cyclooxygenase-2.
35650335	10	69	theme	p42/p44	1419:1425	arg1	activation					1432:1441	p42/p44 MAPK activation	1419:1441	p42/p44 MAPK activation	1419:1441	Reduction of intracellular ROS or attenuation of p42/p44 MAPK activation both reduced LPS-mediated COX-2 expression and preadipocyte proliferation.
35650335	11	70	theme	COX-2	1620:1624	arg1	inhibition					1606:1615	the inhibition	1602:1615	the inhibition of COX-2 or PEG2 receptors	1602:1642	Moreover, LPS-induced preadipocyte proliferation and adipogenesis were abolished by the inhibition of COX-2 or PEG2 receptors.
35650335	3	71	theme	child	357:361	arg1	age					363:365	child age	357:365	child age	357:365	The expansion of fat mass in child age includes both hyperplasia and hypertrophy of adipose tissue, suggesting the importance of proliferation and adipogenesis of preadipocytes.
35216187	4	0	theme	obesogenic	484:493	arg1	effects					495:501	the obesogenic effects	480:501	the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose)	480:566	The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose).
35216187	10	1	dep	glucose	1179:1185	arg1	factors					1207:1213	metabolic factors	1197:1213	metabolic factors	1197:1213	In the liver, the mRNA expression of glucose and lipid metabolic factors was affected in mice fed MFFD.
35216187	7	2	theme	metabolic	833:841	arg1	factors					843:849	lipid metabolic factors	827:849	lipid metabolic factors	827:849	Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint.
35216187	4	3	theme	NPGL	506:509	arg1	effects					495:501	the obesogenic effects	480:501	the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose)	480:566	The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose).
35216187	4	4	dep	sucrose	547:553	arg1	i.e.					541:544	i.e.	541:544	i.e.	541:544	The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose).
35216187	4	5	from	effects	495:501	arg1	mice					514:517	mice	514:517	mice fed different sugars (i.e., sucrose or fructose)	514:566	The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose).
35216187	9	6	from	deposition	1086:1095	arg1	livers					1104:1109	the livers	1100:1109	the livers of mice fed MFFD but not MFSD	1100:1139	In contrast, we observed profound fat deposition in the livers of mice fed MFFD but not MFSD.
35216187	8	7	theme	mice	984:987	arg1	tissue					974:979	the white adipose tissue	956:979	the white adipose tissue of mice fed MFFD	956:996	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	3	8	from	differences	368:378	arg1	efficiency					393:402	lipogenic efficiency	383:402	lipogenic efficiency of sugar types by NPGL	383:425	However, differences in lipogenic efficiency of sugar types by NPGL remain unclear.
35216187	5	9	theme	NPGL-precursor	590:603	arg1	Npgl					611:614	Npgl	611:614	Npgl	611:614	We overexpressed the NPGL-precursor gene (Npgl) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD).
35216187	5	9	theme	NPGL-precursor	590:603	arg1	gene					605:608	the NPGL-precursor gene	586:608	the NPGL-precursor gene (Npgl)	586:615	We overexpressed the NPGL-precursor gene (Npgl) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD).
35216187	6	10	theme	body	750:753	arg1	mass					755:758	body mass	750:758	body mass	750:758	Food intake and body mass were measured for 28 days.
35216187	7	11	theme	mRNA	808:811	arg1	expression					813:822	mRNA expression	808:822	mRNA expression	808:822	Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint.
35216187	1	12	theme	liver	176:180	arg1	steatosis					182:190	liver steatosis	176:190	liver steatosis	176:190	Sugar consumption can readily lead to obesity and metabolic diseases such as liver steatosis.
35216187	5	13	theme	medium-fat/medium-fructose	694:719	arg1	MFFD					727:730	MFFD	727:730	MFFD	727:730	We overexpressed the NPGL-precursor gene (Npgl) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD).
35216187	5	13	theme	medium-fat/medium-fructose	694:719	arg1	diet					721:724	a medium-fat/medium-fructose diet	692:724	a medium-fat/medium-fructose diet (MFFD)	692:731	We overexpressed the NPGL-precursor gene (Npgl) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD).
35216187	0	14	theme	Neurosecretory	0:13	arg1	GL					23:24	Neurosecretory Protein GL	0:24	Neurosecretory Protein GL	0:24	Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet.
35216187	7	15	theme	Body	787:790	arg1	composition					792:802	Body composition	787:802	Body composition	787:802	Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint.
35216187	0	16	theme	Protein	15:21	arg1	GL					23:24	Neurosecretory Protein GL	0:24	Neurosecretory Protein GL	0:24	Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet.
35216187	11	17	theme	liver	1266:1270	arg1	steatosis					1272:1280	liver steatosis	1266:1280	liver steatosis	1266:1280	Hence, NPGL induced liver steatosis in mice fed a fructose-rich diet.
35216187	1	18	theme	Sugar	99:103	arg1	consumption					105:115	Sugar consumption	99:115	Sugar consumption	99:115	Sugar consumption can readily lead to obesity and metabolic diseases such as liver steatosis.
35216187	8	19	theme	food	1035:1038	arg1	intake					1040:1045	food intake	1035:1045	food intake	1035:1045	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	3	20	theme	lipogenic	383:391	arg1	efficiency					393:402	lipogenic efficiency	383:402	lipogenic efficiency of sugar types by NPGL	383:425	However, differences in lipogenic efficiency of sugar types by NPGL remain unclear.
35216187	6	21	theme	Food	734:737	arg1	intake					739:744	Food intake	734:744	Food intake	734:744	Food intake and body mass were measured for 28 days.
35216187	2	22	theme	due	320:322	arg1	accumulation					307:318	fat accumulation	303:318	fat accumulation due to the ingestion of sugar by rats	303:356	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	8	23	from	accumulation	940:951	arg1	tissue					974:979	the white adipose tissue	956:979	the white adipose tissue of mice fed MFFD	956:996	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	8	24	theme	adipose	966:972	arg1	tissue					974:979	the white adipose tissue	956:979	the white adipose tissue of mice fed MFFD	956:996	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	5	25	theme	medium-fat/medium-sucrose	651:675	arg1	diet					677:680	a medium-fat/medium-sucrose diet	649:680	a medium-fat/medium-sucrose diet (MFSD)	649:687	We overexpressed the NPGL-precursor gene (Npgl) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD).
35216187	5	25	theme	medium-fat/medium-sucrose	651:675	arg1	MFSD					683:686	MFSD	683:686	MFSD	683:686	We overexpressed the NPGL-precursor gene (Npgl) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD).
35216187	8	26	theme	fat	936:938	arg1	accumulation					940:951	fat accumulation	936:951	fat accumulation in the white adipose tissue of mice fed MFFD	936:996	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	2	27	theme	fat	303:305	arg1	accumulation					307:318	fat accumulation	303:318	fat accumulation due to the ingestion of sugar by rats	303:356	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	10	28	theme	metabolic	1197:1205	arg1	factors					1207:1213	metabolic factors	1197:1213	metabolic factors	1197:1213	In the liver, the mRNA expression of glucose and lipid metabolic factors was affected in mice fed MFFD.
35216187	3	29	theme	sugar	407:411	arg1	types					413:417	sugar types	407:417	sugar types	407:417	However, differences in lipogenic efficiency of sugar types by NPGL remain unclear.
35216187	10	30	theme	glucose	1179:1185	arg1	expression					1165:1174	the mRNA expression	1156:1174	the mRNA expression of glucose and lipid metabolic factors	1156:1213	In the liver, the mRNA expression of glucose and lipid metabolic factors was affected in mice fed MFFD.
35216187	10	31	theme	lipid	1191:1195	arg1	expression					1165:1174	the mRNA expression	1156:1174	the mRNA expression of glucose and lipid metabolic factors	1156:1213	In the liver, the mRNA expression of glucose and lipid metabolic factors was affected in mice fed MFFD.
35216187	0	32	theme	Liver	38:42	arg1	Steatosis					44:52	Liver Steatosis	38:52	Liver Steatosis	38:52	Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet.
35216187	3	33	theme	types	413:417	arg1	efficiency					393:402	lipogenic efficiency	383:402	lipogenic efficiency of sugar types by NPGL	383:425	However, differences in lipogenic efficiency of sugar types by NPGL remain unclear.
35216187	9	34	theme	mice	1114:1117	arg1	livers					1104:1109	the livers	1100:1109	the livers of mice fed MFFD but not MFSD	1100:1139	In contrast, we observed profound fat deposition in the livers of mice fed MFFD but not MFSD.
35216187	4	35	theme	present	447:453	arg1	study					455:459	The present study	443:459	The present study	443:459	The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose).
35216187	2	36	theme	hypothalamic	233:244	arg1	neuropeptide					246:257	a novel hypothalamic neuropeptide	225:257	a novel hypothalamic neuropeptide	225:257	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	2	36	theme	hypothalamic	233:244	arg1	GL					283:284	neurosecretory protein GL	260:284	neurosecretory protein GL (NPGL)	260:291	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	2	37	theme	sugar	344:348	arg1	ingestion					331:339	the ingestion	327:339	the ingestion of sugar by rats	327:356	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	4	38	theme	different	523:531	arg1	sugars					533:538	different sugars	523:538	different sugars (i.e., sucrose or fructose)	523:566	The present study aimed to elucidate the obesogenic effects of NPGL on mice fed different sugars (i.e., sucrose or fructose).
35216187	5	39	theme	mice	640:643	arg1	hypothalamus					624:635	the hypothalamus	620:635	the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD)	620:731	We overexpressed the NPGL-precursor gene (Npgl) in the hypothalamus of mice fed a medium-fat/medium-sucrose diet (MFSD) or a medium-fat/medium-fructose diet (MFFD).
35216187	2	40	theme	novel	227:231	arg1	neuropeptide					246:257	a novel hypothalamic neuropeptide	225:257	a novel hypothalamic neuropeptide	225:257	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	2	40	theme	novel	227:231	arg1	GL					283:284	neurosecretory protein GL	260:284	neurosecretory protein GL (NPGL)	260:291	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	8	41	theme	Npgl	882:885	arg1	overexpression					887:900	Npgl overexpression	882:900	Npgl overexpression	882:900	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	8	42	theme	white	960:964	arg1	tissue					974:979	the white adipose tissue	956:979	the white adipose tissue of mice fed MFFD	956:996	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	10	43	theme	mRNA	1160:1163	arg1	expression					1165:1174	the mRNA expression	1156:1174	the mRNA expression of glucose and lipid metabolic factors	1156:1213	In the liver, the mRNA expression of glucose and lipid metabolic factors was affected in mice fed MFFD.
35216187	9	44	theme	profound	1073:1080	arg1	deposition					1086:1095	profound fat deposition	1073:1095	profound fat deposition in the livers of mice fed MFFD but not MFSD	1073:1139	In contrast, we observed profound fat deposition in the livers of mice fed MFFD but not MFSD.
35216187	7	45	theme	factors	843:849	arg1	composition					792:802	Body composition	787:802	Body composition	787:802	Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint.
35216187	7	45	theme	factors	843:849	arg1	expression					813:822	mRNA expression	808:822	mRNA expression	808:822	Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint.
35216187	11	46	theme	fructose-rich	1296:1308	arg1	diet					1310:1313	a fructose-rich diet	1294:1313	a fructose-rich diet	1294:1313	Hence, NPGL induced liver steatosis in mice fed a fructose-rich diet.
35216187	0	47	theme	Medium-Fat/Medium-Fructose	66:91	arg1	Diet					93:96	Medium-Fat/Medium-Fructose Diet	66:96	Medium-Fat/Medium-Fructose Diet	66:96	Neurosecretory Protein GL Accelerates Liver Steatosis in Mice Fed Medium-Fat/Medium-Fructose Diet.
35216187	2	48	theme	protein	275:281	arg1	neuropeptide					246:257	a novel hypothalamic neuropeptide	225:257	a novel hypothalamic neuropeptide	225:257	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	2	48	theme	protein	275:281	arg1	NPGL					287:290	NPGL	287:290	NPGL	287:290	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	2	48	theme	protein	275:281	arg1	GL					283:284	neurosecretory protein GL	260:284	neurosecretory protein GL (NPGL)	260:291	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	9	49	theme	fat	1082:1084	arg1	deposition					1086:1095	profound fat deposition	1073:1095	profound fat deposition in the livers of mice fed MFFD but not MFSD	1073:1139	In contrast, we observed profound fat deposition in the livers of mice fed MFFD but not MFSD.
35216187	1	50	theme	metabolic	149:157	arg1	diseases					159:166	metabolic diseases	149:166	metabolic diseases such as liver steatosis	149:190	Sugar consumption can readily lead to obesity and metabolic diseases such as liver steatosis.
35216187	1	50	theme	metabolic	149:157	arg1	steatosis					182:190	liver steatosis	176:190	liver steatosis	176:190	Sugar consumption can readily lead to obesity and metabolic diseases such as liver steatosis.
35216187	2	51	theme	neurosecretory	260:273	arg1	neuropeptide					246:257	a novel hypothalamic neuropeptide	225:257	a novel hypothalamic neuropeptide	225:257	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	2	51	theme	neurosecretory	260:273	arg1	NPGL					287:290	NPGL	287:290	NPGL	287:290	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	2	51	theme	neurosecretory	260:273	arg1	GL					283:284	neurosecretory protein GL	260:284	neurosecretory protein GL (NPGL)	260:291	We previously demonstrated that a novel hypothalamic neuropeptide, neurosecretory protein GL (NPGL), promotes fat accumulation due to the ingestion of sugar by rats.
35216187	8	52	theme	body	921:924	arg1	mass					926:929	body mass	921:929	body mass	921:929	Npgl overexpression potently increased body mass with fat accumulation in the white adipose tissue of mice fed MFFD, although it did not markedly affect food intake.
35216187	7	53	theme	lipid	827:831	arg1	factors					843:849	lipid metabolic factors	827:849	lipid metabolic factors	827:849	Body composition and mRNA expression of lipid metabolic factors were measured at the endpoint.
36270512	0	0	theme	rice	134:137	arg1	endosperm					139:147	rice endosperm	134:147	rice endosperm	134:147	Fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase regulates energy metabolism and synthesis of storage products in developing rice endosperm.
36270512	3	1	from	eukaryotes	459:468	arg1	reactions					403:411	crucial reactions	395:411	crucial reactions of the primary carbohydrate metabolism in all eukaryotes	395:468	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	1	2	theme	dry	191:193	arg1	weight					195:200	the dry weight	187:200	the dry weight of grains	187:210	Starch accounts for about 80-85 % of the dry weight of grains and determines yield by impact on grain weight.
36270512	4	3	theme	sink	598:601	arg1	tissues					603:609	plant sink tissues	592:609	plant sink tissues	592:609	However, the metabolic regulation of starch in plant sink tissues by Fru-2,6-P2 remains unclear.
36270512	5	4	theme	floury	671:676	arg1	flo23					691:695	flo23	691:695	flo23	691:695	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	4	theme	floury	671:676	arg1	mutant					664:669	rice mutant floury endosperm23	659:688	rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs)	659:766	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	3	5	from	signal	523:528	arg1	metabolism					533:542	metabolism	533:542	metabolism	533:542	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	1	6	theme	weight	195:200	arg1	%					182:182	about 80-85 %	170:182	about 80-85 % of the dry weight of grains	170:210	Starch accounts for about 80-85 % of the dry weight of grains and determines yield by impact on grain weight.
36270512	1	6	theme	weight	195:200	arg1	weight					195:200	the dry weight	187:200	the dry weight of grains	187:210	Starch accounts for about 80-85 % of the dry weight of grains and determines yield by impact on grain weight.
36270512	7	7	theme	complementation	872:886	arg1	experiments					888:898	Map-based cloning and genetic complementation experiments	842:898	Map-based cloning and genetic complementation experiments	842:898	Map-based cloning and genetic complementation experiments showed that FLO23 encodes a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP).
36270512	4	8	theme	plant	592:596	arg1	tissues					603:609	plant sink tissues	592:609	plant sink tissues	592:609	However, the metabolic regulation of starch in plant sink tissues by Fru-2,6-P2 remains unclear.
36270512	5	9	theme	compound	739:746	arg1	grains					755:760	anomalous compound starch grains	729:760	anomalous compound starch grains (SGs)	729:766	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	9	theme	compound	739:746	arg1	SGs					763:765	SGs	763:765	SGs	763:765	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	6	10	theme	starch	813:818	arg1	proteins					832:839	proteins	832:839	proteins	832:839	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	6	10	theme	starch	813:818	arg1	lipids					821:826	lipids	821:826	lipids	821:826	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	6	10	theme	starch	813:818	arg1	contents					801:808	reduced contents	793:808	reduced contents of starch	793:818	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	5	11	theme	starch	748:753	arg1	grains					755:760	anomalous compound starch grains	729:760	anomalous compound starch grains (SGs)	729:766	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	11	theme	starch	748:753	arg1	SGs					763:765	SGs	763:765	SGs	763:765	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	8	12	theme	reduced	1098:1104	arg1	PEP					1127:1129	PEP	1127:1129	PEP	1127:1129	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	8	12	theme	reduced	1098:1104	arg1	phosphoenolpyruvate					1106:1124	phosphoenolpyruvate	1106:1124	phosphoenolpyruvate (PEP)	1106:1130	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	2	13	theme	starch	296:301	arg1	composition					281:291	composition	281:291	composition	281:291	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
36270512	2	13	theme	starch	296:301	arg1	content					269:275	content	269:275	content	269:275	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
36270512	8	14	from	content	1047:1053	arg1	cells					1068:1072	endosperm cells	1058:1072	endosperm cells	1058:1072	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	6	15	contain	had	789:791	arg2	lipids					821:826	lipids	821:826	lipids	821:826	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	6	15	contain	had	789:791	arg2	proteins					832:839	proteins	832:839	proteins	832:839	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	6	15	contain	had	789:791	arg1	grains					782:787	flo23 mutant grains	769:787	flo23 mutant grains	769:787	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	6	15	contain	had	789:791	arg2	contents					801:808	reduced contents	793:808	reduced contents of starch	793:818	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	7	16	theme	genetic	864:870	arg1	complementation					872:886	genetic complementation	864:886	genetic complementation	864:886	Map-based cloning and genetic complementation experiments showed that FLO23 encodes a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP).
36270512	6	17	theme	reduced	793:799	arg1	proteins					832:839	proteins	832:839	proteins	832:839	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	6	17	theme	reduced	793:799	arg1	lipids					821:826	lipids	821:826	lipids	821:826	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	6	17	theme	reduced	793:799	arg1	contents					801:808	reduced contents	793:808	reduced contents of starch	793:818	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	7	18	theme	Map-based	842:850	arg1	cloning					852:858	Map-based cloning	842:858	Map-based cloning	842:858	Map-based cloning and genetic complementation experiments showed that FLO23 encodes a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP).
36270512	1	19	theme	grains	205:210	arg1	weight					195:200	the dry weight	187:200	the dry weight of grains	187:210	Starch accounts for about 80-85 % of the dry weight of grains and determines yield by impact on grain weight.
36270512	3	20	theme	crucial	395:401	arg1	reactions					403:411	crucial reactions	395:411	crucial reactions of the primary carbohydrate metabolism in all eukaryotes	395:468	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	3	21	from	reactions	403:411	arg1	eukaryotes					459:468	all eukaryotes	455:468	all eukaryotes	455:468	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	8	22	theme	endosperm	1058:1066	arg1	cells					1068:1072	endosperm cells	1058:1072	endosperm cells	1058:1072	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	7	23	theme	cloning	852:858	arg1	experiments					888:898	Map-based cloning and genetic complementation experiments	842:898	Map-based cloning and genetic complementation experiments	842:898	Map-based cloning and genetic complementation experiments showed that FLO23 encodes a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP).
36270512	3	24	theme	metabolism	441:450	arg1	reactions					403:411	crucial reactions	395:411	crucial reactions of the primary carbohydrate metabolism in all eukaryotes	395:468	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	5	25	theme	rice	659:662	arg1	flo23					691:695	flo23	691:695	flo23	691:695	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	25	theme	rice	659:662	arg1	mutant					664:669	rice mutant floury endosperm23	659:688	rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs)	659:766	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	2	26	dep	content	269:275	arg1	the					265:267	the	265:267	the	265:267	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
36270512	5	27	theme	opaque	708:713	arg1	endosperm					715:723	opaque endosperm	708:723	opaque endosperm	708:723	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	6	28	theme	mutant	775:780	arg1	grains					782:787	flo23 mutant grains	769:787	flo23 mutant grains	769:787	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	8	29	theme	Fru-2,6-P2	1036:1045	arg1	content					1047:1053	Fru-2,6-P2 content	1036:1053	Fru-2,6-P2 content in endosperm cells	1036:1072	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	0	30	theme	energy	68:73	arg1	metabolism					75:84	energy metabolism	68:84	energy metabolism	68:84	Fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase regulates energy metabolism and synthesis of storage products in developing rice endosperm.
36270512	3	31	theme	primary	420:426	arg1	metabolism					441:450	the primary carbohydrate metabolism	416:450	the primary carbohydrate metabolism in all eukaryotes	416:468	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	4	32	theme	starch	582:587	arg1	regulation					568:577	the metabolic regulation	554:577	the metabolic regulation of starch in plant sink tissues by Fru-2,6-P2	554:623	However, the metabolic regulation of starch in plant sink tissues by Fru-2,6-P2 remains unclear.
36270512	8	33	theme	disordered	1158:1167	arg1	glycolysis					1169:1178	glycolysis	1169:1178	glycolysis	1169:1178	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	3	34	theme	carbohydrate	428:439	arg1	metabolism					441:450	the primary carbohydrate metabolism	416:450	the primary carbohydrate metabolism in all eukaryotes	416:468	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	5	35	contain	has	704:706	arg2	endosperm					715:723	opaque endosperm	708:723	opaque endosperm	708:723	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	35	contain	has	704:706	arg1	flo23					691:695	flo23	691:695	flo23	691:695	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	35	contain	has	704:706	arg2	SGs					763:765	SGs	763:765	SGs	763:765	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	35	contain	has	704:706	arg1	mutant					664:669	rice mutant floury endosperm23	659:688	rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs)	659:766	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	35	contain	has	704:706	arg2	grains					755:760	anomalous compound starch grains	729:760	anomalous compound starch grains (SGs)	729:766	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	36	theme	anomalous	729:737	arg1	grains					755:760	anomalous compound starch grains	729:760	anomalous compound starch grains (SGs)	729:766	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	5	36	theme	anomalous	729:737	arg1	SGs					763:765	SGs	763:765	SGs	763:765	Here we isolated rice mutant floury endosperm23 (flo23) which has opaque endosperm and anomalous compound starch grains (SGs).
36270512	1	37	from	impact	236:241	arg1	weight					252:257	grain weight	246:257	grain weight	246:257	Starch accounts for about 80-85 % of the dry weight of grains and determines yield by impact on grain weight.
36270512	0	38	theme	storage	103:109	arg1	products					111:118	storage products	103:118	storage products	103:118	Fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase regulates energy metabolism and synthesis of storage products in developing rice endosperm.
36270512	8	39	dep	phosphoenolpyruvate	1106:1124	arg1	contents					1145:1152	contents	1145:1152	contents	1145:1152	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	9	40	theme	grain	1325:1329	arg1	compounds					1339:1347	grain storage compounds	1325:1347	grain storage compounds	1325:1347	The results imply that OsF2KP2 participates in the glycolytic pathway by providing precursors and energy for synthesis of grain storage compounds.
36270512	2	41	theme	nutritional	350:360	arg1	quality					362:368	nutritional quality	350:368	nutritional quality	350:368	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
36270512	4	42	theme	metabolic	558:566	arg1	regulation					568:577	the metabolic regulation	554:577	the metabolic regulation of starch in plant sink tissues by Fru-2,6-P2	554:623	However, the metabolic regulation of starch in plant sink tissues by Fru-2,6-P2 remains unclear.
36270512	9	43	theme	storage	1331:1337	arg1	compounds					1339:1347	grain storage compounds	1325:1347	grain storage compounds	1325:1347	The results imply that OsF2KP2 participates in the glycolytic pathway by providing precursors and energy for synthesis of grain storage compounds.
36270512	3	44	theme	traffic	515:521	arg1	fructose-2,6-bisphosphate					471:495	fructose-2,6-bisphosphate	471:495	fructose-2,6-bisphosphate (Fru-2,6-P2)	471:508	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	3	44	theme	traffic	515:521	arg1	signal					523:528	a traffic signal	513:528	a traffic signal in metabolism	513:542	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	9	45	theme	compounds	1339:1347	arg1	synthesis					1312:1320	synthesis	1312:1320	synthesis of grain storage compounds	1312:1347	The results imply that OsF2KP2 participates in the glycolytic pathway by providing precursors and energy for synthesis of grain storage compounds.
36270512	9	46	theme	glycolytic	1254:1263	arg1	pathway					1265:1271	the glycolytic pathway	1250:1271	the glycolytic pathway	1250:1271	The results imply that OsF2KP2 participates in the glycolytic pathway by providing precursors and energy for synthesis of grain storage compounds.
36270512	7	47	theme	cytoplasmic	928:938	arg1	F2KP					999:1002	F2KP	999:1002	F2KP	999:1002	Map-based cloning and genetic complementation experiments showed that FLO23 encodes a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP).
36270512	7	47	theme	cytoplasmic	928:938	arg1	Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase					940:996	a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase	926:996	a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP)	926:1003	Map-based cloning and genetic complementation experiments showed that FLO23 encodes a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP).
36270512	7	47	theme	cytoplasmic	928:938	arg1	FLO23					912:916	FLO23	912:916	FLO23	912:916	Map-based cloning and genetic complementation experiments showed that FLO23 encodes a cytoplasmic Fructose-6-phosphate-2-kinase/Fructose-2,6-bisphosphatase (F2KP).
36270512	1	48	theme	grain	246:250	arg1	weight					252:257	grain weight	246:257	grain weight	246:257	Starch accounts for about 80-85 % of the dry weight of grains and determines yield by impact on grain weight.
36270512	8	49	theme	energy	1184:1189	arg1	metabolism					1191:1200	energy metabolism	1184:1200	energy metabolism	1184:1200	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	6	50	theme	flo23	769:773	arg1	grains					782:787	flo23 mutant grains	769:787	flo23 mutant grains	769:787	flo23 mutant grains had reduced contents of starch, lipids and proteins.
36270512	3	51	from	metabolism	441:450	arg1	eukaryotes					459:468	all eukaryotes	455:468	all eukaryotes	455:468	By coordinating crucial reactions of the primary carbohydrate metabolism in all eukaryotes, fructose-2,6-bisphosphate (Fru-2,6-P2) is a traffic signal in metabolism.
36270512	0	52	theme	products	111:118	arg1	synthesis					90:98	synthesis	90:98	synthesis	90:98	Fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase regulates energy metabolism and synthesis of storage products in developing rice endosperm.
36270512	0	52	theme	products	111:118	arg1	metabolism					75:84	energy metabolism	68:84	energy metabolism	68:84	Fructose-6-phosphate-2-kinase/fructose-2,6-bisphosphatase regulates energy metabolism and synthesis of storage products in developing rice endosperm.
36270512	4	53	from	regulation	568:577	arg1	tissues					603:609	plant sink tissues	592:609	plant sink tissues	592:609	However, the metabolic regulation of starch in plant sink tissues by Fru-2,6-P2 remains unclear.
36270512	8	54	theme	OsF2KP2	1018:1024	arg1	Mutation					1006:1013	Mutation	1006:1013	Mutation of OsF2KP2	1006:1024	Mutation of OsF2KP2 decreased Fru-2,6-P2 content in endosperm cells, leading to drastically reduced phosphoenolpyruvate (PEP) and pyruvate contents and disordered glycolysis and energy metabolism.
36270512	2	55	theme	rice	373:376	arg1	appearance					318:327	appearance	318:327	appearance	318:327	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
36270512	2	55	theme	rice	373:376	arg1	cooking					338:344	cooking	338:344	cooking	338:344	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
36270512	2	55	theme	rice	373:376	arg1	quality					362:368	nutritional quality	350:368	nutritional quality	350:368	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
36270512	2	55	theme	rice	373:376	arg1	eating					330:335	eating	330:335	eating	330:335	And, the content and composition of starch also determine appearance, eating, cooking and nutritional quality of rice.
35234309	0	0	theme	gut	80:82	arg1	microbiota					84:93	gut microbiota	80:93	gut microbiota	80:93	Curcumin regulates the homeostasis of Th17/Treg and improves the composition of gut microbiota in type 2 diabetic mice with colitis.
35234309	8	1	theme	intestinal	1345:1354	arg1	microbiota					1356:1365	the intestinal microbiota	1341:1365	the intestinal microbiota	1341:1365	Collectively, curcumin effectively alleviated colitis in mice with type 2 diabetes mellitus by restoring the homeostasis of Th17/Treg and improving the composition of the intestinal microbiota.
35234309	7	2	dep	diversity	1028:1036	arg1	the					1024:1026	the	1024:1026	the	1024:1026	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	1	3	theme	ulcerative	213:222	arg1	colitis					224:230	ulcerative colitis	213:230	ulcerative colitis (UC)	213:235	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	1	3	theme	ulcerative	213:222	arg1	UC					233:234	UC	233:234	UC	233:234	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	5	4	theme	lighter	780:786	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	4	theme	lighter	780:786	arg1	colons					788:793	longer and lighter colons	769:793	longer and lighter colons	769:793	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	5	theme	longer	769:774	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	5	theme	longer	769:774	arg1	colons					788:793	longer and lighter colons	769:793	longer and lighter colons	769:793	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	8	6	with	mice	1231:1234	arg1	mellitus					1257:1264	type 2 diabetes mellitus	1241:1264	type 2 diabetes mellitus	1241:1264	Collectively, curcumin effectively alleviated colitis in mice with type 2 diabetes mellitus by restoring the homeostasis of Th17/Treg and improving the composition of the intestinal microbiota.
35234309	7	7	theme	microbiota	1075:1084	arg1	diversity					1028:1036	diversity	1028:1036	diversity	1028:1036	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	7	7	theme	microbiota	1075:1084	arg1	abundance					1051:1059	relative abundance	1042:1059	relative abundance	1042:1059	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	7	8	theme	family	1149:1154	arg1	levels					1166:1171	the phylum, class, order, family and genus levels	1123:1171	levels	1166:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	5	9	theme	less	821:824	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	9	theme	less	821:824	arg1	infiltration					844:855	less inflammatory cell infiltration	821:855	less inflammatory cell infiltration	821:855	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	0	10	theme	microbiota	84:93	arg1	composition					65:75	the composition	61:75	the composition of gut microbiota in type 2 diabetic mice with colitis	61:130	Curcumin regulates the homeostasis of Th17/Treg and improves the composition of gut microbiota in type 2 diabetic mice with colitis.
35234309	7	11	from	diversity	1028:1036	arg1	mice					1089:1092	mice	1089:1092	mice with UC complicated by DM at the phylum, class, order, family and genus levels	1089:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	5	12	with	symptoms	685:692	arg1	level					746:750	a lower insulin level	730:750	a lower insulin level	730:750	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	13	theme	inflammatory	826:837	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	13	theme	inflammatory	826:837	arg1	infiltration					844:855	less inflammatory cell infiltration	821:855	less inflammatory cell infiltration	821:855	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	4	14	theme	sodium	602:607	arg1	sulfate					609:615	dextran sodium sulfate	594:615	dextran sodium sulfate	594:615	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
35234309	2	15	used	used	324:327	arg2	adjunct					335:341	an adjunct	332:341	an adjunct to the treatment of UC and DM	332:371	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	2	15	used	used	324:327	arg2	Curcumin					238:245	Curcumin	238:245	Curcumin	238:245	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	5	16	theme	cell	839:842	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	16	theme	cell	839:842	arg1	infiltration					844:855	less inflammatory cell infiltration	821:855	less inflammatory cell infiltration	821:855	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	4	17	theme	DB	556:557	arg1	mice					559:562	DB mice	556:562	DB mice (spontaneously diabetic) with dextran sodium sulfate	556:615	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
35234309	7	18	theme	intestinal	1064:1073	arg1	microbiota					1075:1084	intestinal microbiota	1064:1084	intestinal microbiota	1064:1084	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	5	19	theme	insulin	738:744	arg1	level					746:750	a lower insulin level	730:750	a lower insulin level	730:750	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	7	20	theme	relative	1042:1049	arg1	abundance					1051:1059	relative abundance	1042:1059	relative abundance	1042:1059	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	7	21	from	abundance	1051:1059	arg1	mice					1089:1092	mice	1089:1092	mice with UC complicated by DM at the phylum, class, order, family and genus levels	1089:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	6	22	theme	Th17	948:951	arg1	responses					953:961	weaker Th17 responses	941:961	weaker Th17 responses	941:961	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	5	23	theme	fewer	796:800	arg1	ulcers					810:815	fewer mucosal ulcers	796:815	fewer mucosal ulcers	796:815	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	23	theme	fewer	796:800	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	7	24	theme	genus	1160:1164	arg1	levels					1166:1171	the phylum, class, order, family and genus levels	1123:1171	levels	1166:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	5	25	theme	diabetes	697:704	arg1	ulcers					810:815	fewer mucosal ulcers	796:815	fewer mucosal ulcers	796:815	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	25	theme	diabetes	697:704	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	25	theme	diabetes	697:704	arg1	colons					788:793	longer and lighter colons	769:793	longer and lighter colons	769:793	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	25	theme	diabetes	697:704	arg1	weight					761:766	heavier weight	753:766	heavier weight	753:766	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	25	theme	diabetes	697:704	arg1	infiltration					844:855	less inflammatory cell infiltration	821:855	less inflammatory cell infiltration	821:855	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	26	theme	mucosal	802:808	arg1	ulcers					810:815	fewer mucosal ulcers	796:815	fewer mucosal ulcers	796:815	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	26	theme	mucosal	802:808	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	2	27	theme	DM	370:371	arg1	treatment					350:358	the treatment	346:358	the treatment of UC and DM	346:371	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	4	28	theme	dextran	594:600	arg1	sulfate					609:615	dextran sodium sulfate	594:615	dextran sodium sulfate	594:615	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
35234309	2	29	theme	wide	253:256	arg1	range					258:262	a wide range	251:262	a wide range of bioactive and pharmacological properties	251:306	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	2	30	theme	UC	363:364	arg1	treatment					350:358	the treatment	346:358	the treatment of UC and DM	346:371	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	0	31	theme	diabetic	105:112	arg1	mice					114:117	type 2 diabetic mice	98:117	type 2 diabetic mice with colitis	98:130	Curcumin regulates the homeostasis of Th17/Treg and improves the composition of gut microbiota in type 2 diabetic mice with colitis.
35234309	8	32	theme	Th17/Treg	1298:1306	arg1	homeostasis					1283:1293	the homeostasis	1279:1293	the homeostasis of Th17/Treg	1279:1306	Collectively, curcumin effectively alleviated colitis in mice with type 2 diabetes mellitus by restoring the homeostasis of Th17/Treg and improving the composition of the intestinal microbiota.
35234309	4	33	theme	diabetes	529:536	arg1	model					504:508	a model	502:508	a model of UC complicating diabetes	502:536	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
35234309	0	34	theme	Th17/Treg	38:46	arg1	homeostasis					23:33	the homeostasis	19:33	the homeostasis of Th17/Treg	19:46	Curcumin regulates the homeostasis of Th17/Treg and improves the composition of gut microbiota in type 2 diabetic mice with colitis.
35234309	2	35	contain	has	247:249	arg1	Curcumin					238:245	Curcumin	238:245	Curcumin	238:245	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	2	35	contain	has	247:249	arg1	adjunct					335:341	an adjunct	332:341	an adjunct to the treatment of UC and DM	332:371	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	2	35	contain	has	247:249	arg2	range					258:262	a wide range	251:262	a wide range of bioactive and pharmacological properties	251:306	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	4	36	with	mice	559:562	arg1	sulfate					609:615	dextran sodium sulfate	594:615	dextran sodium sulfate	594:615	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
35234309	7	37	theme	order	1142:1146	arg1	levels					1166:1171	the phylum, class, order, family and genus levels	1123:1171	levels	1166:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	5	38	theme	lower	732:736	arg1	level					746:750	a lower insulin level	730:750	a lower insulin level	730:750	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	0	39	from	composition	65:75	arg1	mice					114:117	type 2 diabetic mice	98:117	type 2 diabetic mice with colitis	98:130	Curcumin regulates the homeostasis of Th17/Treg and improves the composition of gut microbiota in type 2 diabetic mice with colitis.
35234309	6	40	theme	untreated	880:888	arg1	mice					893:896	untreated DB mice	880:896	untreated DB mice with colitis	880:909	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	1	41	theme	common	175:180	arg1	complications					182:194	the most common complications	166:194	the most common complications in patients with ulcerative colitis (UC)	166:235	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	5	42	theme	heavier	753:759	arg1	symptoms					685:692	the symptoms	681:692	the symptoms of diabetes complicated by UC	681:722	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	5	42	theme	heavier	753:759	arg1	weight					761:766	heavier weight	753:766	heavier weight	753:766	In this study, curcumin (100 mg/kg/day) significantly improved the symptoms of diabetes complicated by UC, with a lower insulin level, heavier weight, longer and lighter colons, fewer mucosal ulcers and less inflammatory cell infiltration.
35234309	7	43	theme	class	1135:1139	arg1	levels					1166:1171	the phylum, class, order, family and genus levels	1123:1171	levels	1166:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	6	44	with	mice	893:896	arg1	colitis					903:909	colitis	903:909	colitis	903:909	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	6	45	theme	Treg	976:979	arg1	responses					981:989	stronger Treg responses	967:989	stronger Treg responses	967:989	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	2	46	theme	properties	297:306	arg1	range					258:262	a wide range	251:262	a wide range of bioactive and pharmacological properties	251:306	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	8	47	theme	diabetes	1248:1255	arg1	mellitus					1257:1264	type 2 diabetes mellitus	1241:1264	type 2 diabetes mellitus	1241:1264	Collectively, curcumin effectively alleviated colitis in mice with type 2 diabetes mellitus by restoring the homeostasis of Th17/Treg and improving the composition of the intestinal microbiota.
35234309	3	48	from	role	387:390	arg1	UC					407:408	UC	407:408	UC complicated by DM	407:426	However, the role of curcumin in UC complicated by DM has not been elucidated.
35234309	2	49	theme	pharmacological	281:295	arg1	properties					297:306	bioactive and pharmacological properties	267:306	bioactive and pharmacological properties	267:306	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	1	50	from	complications	182:194	arg1	patients					199:206	patients	199:206	patients with ulcerative colitis (UC)	199:235	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	7	51	with	mice	1089:1092	arg1	UC					1099:1100	UC	1099:1100	UC complicated by DM at the phylum, class, order, family and genus levels	1099:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	8	52	theme	type	1241:1244	arg1	mellitus					1257:1264	type 2 diabetes mellitus	1241:1264	type 2 diabetes mellitus	1241:1264	Collectively, curcumin effectively alleviated colitis in mice with type 2 diabetes mellitus by restoring the homeostasis of Th17/Treg and improving the composition of the intestinal microbiota.
35234309	3	53	theme	curcumin	395:402	arg1	role					387:390	the role	383:390	the role of curcumin in UC complicated by DM	383:426	However, the role of curcumin in UC complicated by DM has not been elucidated.
35234309	1	54	theme	complications	182:194	arg1	complications					182:194	the most common complications	166:194	the most common complications in patients with ulcerative colitis (UC)	166:235	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	1	54	theme	complications	182:194	arg1	one					159:161	one	159:161	one	159:161	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	1	55	with	patients	199:206	arg1	colitis					224:230	ulcerative colitis	213:230	ulcerative colitis (UC)	213:235	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	1	55	with	patients	199:206	arg1	UC					233:234	UC	233:234	UC	233:234	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	2	56	theme	bioactive	267:275	arg1	properties					297:306	bioactive and pharmacological properties	267:306	bioactive and pharmacological properties	267:306	Curcumin has a wide range of bioactive and pharmacological properties and is commonly used as an adjunct to the treatment of UC and DM.
35234309	0	57	with	mice	114:117	arg1	colitis					124:130	colitis	124:130	colitis	124:130	Curcumin regulates the homeostasis of Th17/Treg and improves the composition of gut microbiota in type 2 diabetic mice with colitis.
35234309	6	58	theme	DB	890:891	arg1	mice					893:896	untreated DB mice	880:896	untreated DB mice with colitis	880:909	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	6	59	theme	stronger	967:974	arg1	responses					981:989	stronger Treg responses	967:989	stronger Treg responses	967:989	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	6	60	theme	curcumin-treated	912:927	arg1	mice					929:932	curcumin-treated mice	912:932	curcumin-treated mice	912:932	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	4	61	theme	diabetic	579:586	arg1	mice					559:562	DB mice	556:562	DB mice (spontaneously diabetic) with dextran sodium sulfate	556:615	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
35234309	6	62	theme	weaker	941:946	arg1	responses					953:961	weaker Th17 responses	941:961	weaker Th17 responses	941:961	Moreover, compared to untreated DB mice with colitis, curcumin-treated mice showed weaker Th17 responses and stronger Treg responses.
35234309	4	63	theme	complicating	516:527	arg1	diabetes					529:536	UC complicating diabetes	513:536	UC complicating diabetes	513:536	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
35234309	8	64	theme	microbiota	1356:1365	arg1	composition					1326:1336	the composition	1322:1336	the composition of the intestinal microbiota	1322:1365	Collectively, curcumin effectively alleviated colitis in mice with type 2 diabetes mellitus by restoring the homeostasis of Th17/Treg and improving the composition of the intestinal microbiota.
35234309	7	65	theme	phylum	1127:1132	arg1	levels					1166:1171	the phylum, class, order, family and genus levels	1123:1171	levels	1166:1171	In addition, curcumin regulated the diversity and relative abundance of intestinal microbiota in mice with UC complicated by DM at the phylum, class, order, family and genus levels.
35234309	1	66	theme	Diabetes	133:140	arg1	DM					152:153	DM	152:153	DM	152:153	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	1	66	theme	Diabetes	133:140	arg1	mellitus					142:149	Diabetes mellitus	133:149	Diabetes mellitus (DM)	133:154	Diabetes mellitus (DM) is one of the most common complications in patients with ulcerative colitis (UC).
35234309	4	67	theme	UC	513:514	arg1	diabetes					529:536	UC complicating diabetes	513:536	UC complicating diabetes	513:536	Therefore, this study was conducted to construct a model of UC complicating diabetes by inducing UC in DB mice (spontaneously diabetic) with dextran sodium sulfate.
36555723	4	0	theme	mutant	782:787	arg1	lines					796:800	two mutant pmei12 lines	778:800	two mutant pmei12 lines	778:800	Furthermore, CRISPR/Cas9 was used to edit the OsPMEI12 (LOC_Os03G01020) and two mutant pmei12 lines were obtained to explore the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress.
36555723	3	1	theme	promoter	596:603	arg1	region					605:610	the promoter region	592:610	the promoter region	592:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	2	2	theme	Pectin	305:310	arg1	methylesterase					312:325	Pectin methylesterase	305:325	Pectin methylesterase (PME)	305:331	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	2	theme	Pectin	305:310	arg1	inhibitor					359:367	pectin methylesterase inhibitor	337:367	pectin methylesterase inhibitor (PMEI)	337:374	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	2	theme	Pectin	305:310	arg1	factors					399:405	the most important factors	380:405	the most important factors for modifying methyl esterification	380:441	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	2	theme	Pectin	305:310	arg1	PME					328:330	PME	328:330	PME	328:330	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	7	3	dep	both	1324:1327	arg1	seedling					1329:1336	seedling	1329:1336	seedling	1329:1336	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	7	4	theme	cell	1301:1304	arg1	wall					1306:1309	the cell wall	1297:1309	the cell wall	1297:1309	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	3	5	theme	gene	639:642	arg1	duplication					644:654	gene duplication	639:654	gene duplication	639:654	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	1	6	theme	middle	222:227	arg1	lamella					229:235	middle lamella	222:235	middle lamella	222:235	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	7	7	theme	pectin	1279:1284	arg1	content					1286:1292	the pectin content	1275:1292	the pectin content of the cell wall	1275:1309	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	3	8	theme	phylogenetic	661:672	arg1	relationship					674:685	phylogenetic relationship	661:685	phylogenetic relationship	661:685	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	5	9	from	reduction	1048:1056	arg1	height					1067:1072	plant height	1061:1072	plant height at mature stage	1061:1088	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	5	9	from	reduction	1048:1056	arg1	stage					1084:1088	mature stage	1077:1088	mature stage	1077:1088	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	7	10	theme	pmei12	1232:1237	arg1	lines					1239:1243	pmei12 lines	1232:1243	pmei12 lines	1232:1243	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	3	11	from	duplication	644:654	arg1	region					605:610	the promoter region	592:610	the promoter region	592:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	6	12	theme	mutant	1178:1183	arg1	plants					1185:1190	mutant plants	1178:1190	mutant plants	1178:1190	The seed setting rate, and fresh and dry weights of the mutants were also decreased in mutant plants.
36555723	8	13	theme	phytohormone	1543:1554	arg1	treatments					1570:1579	two phytohormone (GA3 and NAA) treatments	1539:1579	two phytohormone (GA3 and NAA) treatments at seedling stage	1539:1597	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	13	theme	phytohormone	1543:1554	arg1	NAA					1565:1567	NAA	1565:1567	NAA	1565:1567	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	13	theme	phytohormone	1543:1554	arg1	GA3					1557:1559	GA3	1557:1559	GA3	1557:1559	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	1	14	theme	cell	170:173	arg1	wall					175:178	the cell wall	166:178	the cell wall	166:178	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	4	15	theme	OsPMEI	844:849	arg1	functions					831:839	the functions	827:839	the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress	827:912	Furthermore, CRISPR/Cas9 was used to edit the OsPMEI12 (LOC_Os03G01020) and two mutant pmei12 lines were obtained to explore the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress.
36555723	9	16	theme	pmei12	1661:1666	arg1	lines					1668:1672	pmei12 lines	1661:1672	pmei12 lines	1661:1672	Under Cd stress, the fresh and dry weights were increased in pmei12 lines.
36555723	6	17	theme	setting	1100:1106	arg1	rate					1108:1111	The seed setting rate	1091:1111	The seed setting rate	1091:1111	The seed setting rate, and fresh and dry weights of the mutants were also decreased in mutant plants.
36555723	0	18	theme	Metal	115:119	arg1	Stress					121:126	Heavy Metal Stress	109:126	Heavy Metal Stress	109:126	CRISPR/Cas9 Mutant Rice Ospmei12 Involved in Growth, Cell Wall Development, and Response to Phytohormone and Heavy Metal Stress.
36555723	4	19	from	functions	831:839	arg1	growth					860:865	plant growth	854:865	plant growth	854:865	Furthermore, CRISPR/Cas9 was used to edit the OsPMEI12 (LOC_Os03G01020) and two mutant pmei12 lines were obtained to explore the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress.
36555723	3	20	theme	bioinformatics	518:531	arg1	tools					533:537	bioinformatics tools	518:537	bioinformatics tools	518:537	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	10	21	theme	esterification	1754:1767	arg1	regulation					1733:1742	the regulation	1729:1742	the regulation of methyl esterification during growth	1729:1781	These results indicated that OsPMEI12 was involved in the regulation of methyl esterification during growth, affected cell wall composition and agronomic traits, and might play an important role in responses to phytohormones and stress.
36555723	5	22	theme	wild	927:930	arg1	WT					938:939	WT	938:939	WT	938:939	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	5	22	theme	wild	927:930	arg1	type					932:935	wild type	927:935	wild type (WT)	927:940	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	10	23	theme	wall	1798:1801	arg1	composition					1803:1813	cell wall composition	1793:1813	cell wall composition	1793:1813	These results indicated that OsPMEI12 was involved in the regulation of methyl esterification during growth, affected cell wall composition and agronomic traits, and might play an important role in responses to phytohormones and stress.
36555723	0	24	theme	Cell	53:56	arg1	Development					63:73	Cell Wall Development	53:73	Cell Wall Development	53:73	CRISPR/Cas9 Mutant Rice Ospmei12 Involved in Growth, Cell Wall Development, and Response to Phytohormone and Heavy Metal Stress.
36555723	1	25	theme	cell	283:286	arg1	stickiness					293:302	the cell wall stickiness	279:302	the cell wall stickiness	279:302	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	8	26	theme	seedling	1584:1591	arg1	stage					1593:1597	seedling stage	1584:1597	seedling stage	1584:1597	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	5	27	theme	plants	999:1004	arg1	internodes					974:983	the second inverted internodes	954:983	the second inverted internodes of the mutant plants	954:1004	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	4	28	theme	plant	854:858	arg1	growth					860:865	plant growth	854:865	plant growth	854:865	Furthermore, CRISPR/Cas9 was used to edit the OsPMEI12 (LOC_Os03G01020) and two mutant pmei12 lines were obtained to explore the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress.
36555723	5	29	theme	second	958:963	arg1	internodes					974:983	the second inverted internodes	954:983	the second inverted internodes of the mutant plants	954:1004	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	2	30	theme	methylesterase	344:357	arg1	methylesterase					312:325	Pectin methylesterase	305:325	Pectin methylesterase (PME)	305:331	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	30	theme	methylesterase	344:357	arg1	PMEI					370:373	PMEI	370:373	PMEI	370:373	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	30	theme	methylesterase	344:357	arg1	factors					399:405	the most important factors	380:405	the most important factors for modifying methyl esterification	380:441	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	30	theme	methylesterase	344:357	arg1	inhibitor					359:367	pectin methylesterase inhibitor	337:367	pectin methylesterase inhibitor (PMEI)	337:374	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	31	theme	important	389:397	arg1	methylesterase					312:325	Pectin methylesterase	305:325	Pectin methylesterase (PME)	305:331	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	31	theme	important	389:397	arg1	inhibitor					359:367	pectin methylesterase inhibitor	337:367	pectin methylesterase inhibitor (PMEI)	337:374	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	31	theme	important	389:397	arg1	factors					399:405	the most important factors	380:405	the most important factors for modifying methyl esterification	380:441	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	5	32	theme	plant	1061:1065	arg1	height					1067:1072	plant height	1061:1072	plant height at mature stage	1061:1088	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	0	33	theme	CRISPR/Cas9	0:10	arg1	Ospmei12					24:31	CRISPR/Cas9 Mutant Rice Ospmei12	0:31	CRISPR/Cas9 Mutant Rice Ospmei12	0:31	CRISPR/Cas9 Mutant Rice Ospmei12 Involved in Growth, Cell Wall Development, and Response to Phytohormone and Heavy Metal Stress.
36555723	1	34	theme	lamella	229:235	arg1	constituents					150:161	the constituents	146:161	the constituents of the cell wall, distributed in the primary cell wall	146:216	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	1	34	theme	lamella	229:235	arg1	lamella					229:235	middle lamella	222:235	middle lamella	222:235	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	1	34	theme	lamella	229:235	arg1	one					139:141	one	139:141	one	139:141	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	5	35	dep	shortened	1006:1014	arg1	Nipponbare					942:951	Nipponbare	942:951	Nipponbare	942:951	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	0	36	theme	Rice	19:22	arg1	Ospmei12					24:31	CRISPR/Cas9 Mutant Rice Ospmei12	0:31	CRISPR/Cas9 Mutant Rice Ospmei12	0:31	CRISPR/Cas9 Mutant Rice Ospmei12 Involved in Growth, Cell Wall Development, and Response to Phytohormone and Heavy Metal Stress.
36555723	2	37	theme	methyl	421:426	arg1	esterification					428:441	methyl esterification	421:441	methyl esterification	421:441	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	1	38	theme	rheological	252:262	arg1	properties					264:273	the rheological properties	248:273	the rheological properties	248:273	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	8	39	dep	treatments	1570:1579	arg1	treatments					1570:1579	two phytohormone (GA3 and NAA) treatments	1539:1579	two phytohormone (GA3 and NAA) treatments at seedling stage	1539:1597	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	39	dep	treatments	1570:1579	arg1	NAA					1565:1567	NAA	1565:1567	NAA	1565:1567	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	39	dep	treatments	1570:1579	arg1	GA3					1557:1559	GA3	1557:1559	GA3	1557:1559	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	40	theme	lines	1474:1478	arg1	growth					1453:1458	the growth	1449:1458	the growth of the pmei12 lines	1449:1478	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	40	theme	lines	1474:1478	arg1	better					1484:1489	better	1484:1489	better	1484:1489	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	7	41	theme	wall	1306:1309	arg1	content					1286:1292	the pectin content	1275:1292	the pectin content of the cell wall	1275:1309	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	3	42	theme	chromosomal	613:623	arg1	distribution					625:636	chromosomal distribution	613:636	chromosomal distribution	613:636	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	1	43	theme	cell	208:211	arg1	wall					213:216	the primary cell wall	196:216	the primary cell wall	196:216	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	5	44	theme	mutant	992:997	arg1	plants					999:1004	the mutant plants	988:1004	the mutant plants	988:1004	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	10	45	theme	important	1855:1863	arg1	role					1865:1868	an important role	1852:1868	an important role	1852:1868	These results indicated that OsPMEI12 was involved in the regulation of methyl esterification during growth, affected cell wall composition and agronomic traits, and might play an important role in responses to phytohormones and stress.
36555723	3	46	from	structure	550:558	arg1	region					605:610	the promoter region	592:610	the promoter region	592:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	6	47	theme	dry	1128:1130	arg1	weights					1132:1138	fresh and dry weights	1118:1138	fresh and dry weights of the mutants	1118:1153	The seed setting rate, and fresh and dry weights of the mutants were also decreased in mutant plants.
36555723	5	48	theme	mature	1077:1082	arg1	stage					1084:1088	mature stage	1077:1088	mature stage	1077:1088	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	4	49	theme	pmei12	789:794	arg1	lines					796:800	two mutant pmei12 lines	778:800	two mutant pmei12 lines	778:800	Furthermore, CRISPR/Cas9 was used to edit the OsPMEI12 (LOC_Os03G01020) and two mutant pmei12 lines were obtained to explore the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress.
36555723	3	50	from	motifs	561:566	arg1	region					605:610	the promoter region	592:610	the promoter region	592:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	6	51	theme	fresh	1118:1122	arg1	weights					1132:1138	fresh and dry weights	1118:1138	fresh and dry weights of the mutants	1118:1153	The seed setting rate, and fresh and dry weights of the mutants were also decreased in mutant plants.
36555723	3	52	from	distribution	625:636	arg1	region					605:610	the promoter region	592:610	the promoter region	592:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	8	53	theme	normal	1511:1516	arg1	conditions					1518:1527	both normal conditions	1506:1527	both normal conditions	1506:1527	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	1	54	theme	constituents	150:161	arg1	constituents					150:161	the constituents	146:161	the constituents of the cell wall, distributed in the primary cell wall	146:216	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	1	54	theme	constituents	150:161	arg1	lamella					229:235	middle lamella	222:235	middle lamella	222:235	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	1	54	theme	constituents	150:161	arg1	one					139:141	one	139:141	one	139:141	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	0	55	theme	Heavy	109:113	arg1	Stress					121:126	Heavy Metal Stress	109:126	Heavy Metal Stress	109:126	CRISPR/Cas9 Mutant Rice Ospmei12 Involved in Growth, Cell Wall Development, and Response to Phytohormone and Heavy Metal Stress.
36555723	4	56	used	used	731:734	arg2	CRISPR/Cas9					715:725	CRISPR/Cas9	715:725	CRISPR/Cas9	715:725	Furthermore, CRISPR/Cas9 was used to edit the OsPMEI12 (LOC_Os03G01020) and two mutant pmei12 lines were obtained to explore the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress.
36555723	7	57	theme	lines	1239:1243	arg1	methylation					1217:1227	the pectin methylation	1206:1227	the pectin methylation of pmei12 lines	1206:1243	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	1	58	theme	wall	175:178	arg1	constituents					150:161	the constituents	146:161	the constituents of the cell wall, distributed in the primary cell wall	146:216	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	1	58	theme	wall	175:178	arg1	lamella					229:235	middle lamella	222:235	middle lamella	222:235	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	6	59	theme	seed	1095:1098	arg1	rate					1108:1111	The seed setting rate	1091:1111	The seed setting rate	1091:1111	The seed setting rate, and fresh and dry weights of the mutants were also decreased in mutant plants.
36555723	7	60	theme	seedling	1418:1425	arg1	stage					1427:1431	seedling stage	1418:1431	seedling stage	1418:1431	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	7	61	theme	pectin	1210:1215	arg1	methylation					1217:1227	the pectin methylation	1206:1227	the pectin methylation of pmei12 lines	1206:1243	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	10	62	theme	methyl	1747:1752	arg1	esterification					1754:1767	methyl esterification	1747:1767	methyl esterification	1747:1767	These results indicated that OsPMEI12 was involved in the regulation of methyl esterification during growth, affected cell wall composition and agronomic traits, and might play an important role in responses to phytohormones and stress.
36555723	6	63	theme	mutants	1147:1153	arg1	rate					1108:1111	The seed setting rate	1091:1111	The seed setting rate	1091:1111	The seed setting rate, and fresh and dry weights of the mutants were also decreased in mutant plants.
36555723	6	63	theme	mutants	1147:1153	arg1	weights					1132:1138	fresh and dry weights	1118:1138	fresh and dry weights of the mutants	1118:1153	The seed setting rate, and fresh and dry weights of the mutants were also decreased in mutant plants.
36555723	8	64	from	stage	1593:1597	arg1	treatments					1570:1579	two phytohormone (GA3 and NAA) treatments	1539:1579	two phytohormone (GA3 and NAA) treatments at seedling stage	1539:1597	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	64	from	stage	1593:1597	arg1	NAA					1565:1567	NAA	1565:1567	NAA	1565:1567	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	8	64	from	stage	1593:1597	arg1	GA3					1557:1559	GA3	1557:1559	GA3	1557:1559	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	10	65	theme	cell	1793:1796	arg1	composition					1803:1813	cell wall composition	1793:1813	cell wall composition	1793:1813	These results indicated that OsPMEI12 was involved in the regulation of methyl esterification during growth, affected cell wall composition and agronomic traits, and might play an important role in responses to phytohormones and stress.
36555723	0	66	theme	Wall	58:61	arg1	Development					63:73	Cell Wall Development	53:73	Cell Wall Development	53:73	CRISPR/Cas9 Mutant Rice Ospmei12 Involved in Growth, Cell Wall Development, and Response to Phytohormone and Heavy Metal Stress.
36555723	3	67	dep	Oryza	484:488	arg1	L.					497:498	Oryza sativa L.	484:498	Oryza sativa L.	484:498	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	1	68	theme	wall	288:291	arg1	stickiness					293:302	the cell wall stickiness	279:302	the cell wall stickiness	279:302	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36555723	5	69	from	stage	1084:1088	arg1	reduction					1048:1056	the reduction	1044:1056	the reduction in plant height at mature stage	1044:1088	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	5	69	from	stage	1084:1088	arg1	height					1067:1072	plant height	1061:1072	plant height at mature stage	1061:1088	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	3	70	from	rice	478:481	arg1	genes					467:471	45 PMEI genes	459:471	45 PMEI genes from rice (Oryza sativa L.)	459:499	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	10	71	theme	agronomic	1819:1827	arg1	traits					1829:1834	agronomic traits	1819:1834	agronomic traits	1819:1834	These results indicated that OsPMEI12 was involved in the regulation of methyl esterification during growth, affected cell wall composition and agronomic traits, and might play an important role in responses to phytohormones and stress.
36555723	5	72	theme	inverted	965:972	arg1	internodes					974:983	the second inverted internodes	954:983	the second inverted internodes of the mutant plants	954:1004	Compared to wild type (WT) Nipponbare, the second inverted internodes of the mutant plants shortened significantly, resulting in the reduction in plant height at mature stage.
36555723	2	73	theme	pectin	337:342	arg1	methylesterase					312:325	Pectin methylesterase	305:325	Pectin methylesterase (PME)	305:331	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	73	theme	pectin	337:342	arg1	PMEI					370:373	PMEI	370:373	PMEI	370:373	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	73	theme	pectin	337:342	arg1	factors					399:405	the most important factors	380:405	the most important factors for modifying methyl esterification	380:441	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	2	73	theme	pectin	337:342	arg1	inhibitor					359:367	pectin methylesterase inhibitor	337:367	pectin methylesterase inhibitor (PMEI)	337:374	Pectin methylesterase (PME) and pectin methylesterase inhibitor (PMEI) are the most important factors for modifying methyl esterification.
36555723	0	74	theme	Mutant	12:17	arg1	Ospmei12					24:31	CRISPR/Cas9 Mutant Rice Ospmei12	0:31	CRISPR/Cas9 Mutant Rice Ospmei12	0:31	CRISPR/Cas9 Mutant Rice Ospmei12 Involved in Growth, Cell Wall Development, and Response to Phytohormone and Heavy Metal Stress.
36555723	3	75	from	relationship	674:685	arg1	region					605:610	the promoter region	592:610	the promoter region	592:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	9	76	theme	Cd	1606:1607	arg1	stress					1609:1614	Cd stress	1606:1614	Cd stress	1606:1614	Under Cd stress, the fresh and dry weights were increased in pmei12 lines.
36555723	7	77	dep	increased	1311:1319	arg1	increased					1400:1408	increased	1400:1408	increased only at seedling stage	1400:1431	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	4	78	theme	cadmium	894:900	arg1	stress					907:912	cadmium (Cd) stress	894:912	cadmium (Cd) stress	894:912	Furthermore, CRISPR/Cas9 was used to edit the OsPMEI12 (LOC_Os03G01020) and two mutant pmei12 lines were obtained to explore the functions of OsPMEI in plant growth and development, and under cadmium (Cd) stress.
36555723	9	79	theme	fresh	1621:1625	arg1	weights					1635:1641	the fresh and dry weights	1617:1641	the fresh and dry weights	1617:1641	Under Cd stress, the fresh and dry weights were increased in pmei12 lines.
36555723	8	80	theme	pmei12	1467:1472	arg1	lines					1474:1478	the pmei12 lines	1463:1478	the pmei12 lines	1463:1478	Interestingly, the growth of the pmei12 lines was better than the WT in both normal conditions and under two phytohormone (GA3 and NAA) treatments at seedling stage.
36555723	9	81	theme	dry	1631:1633	arg1	weights					1635:1641	the fresh and dry weights	1617:1641	the fresh and dry weights	1617:1641	Under Cd stress, the fresh and dry weights were increased in pmei12 lines.
36555723	3	82	theme	cis-acting	569:578	arg1	elements					580:587	cis-acting elements	569:587	cis-acting elements in the promoter region	569:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	7	83	dep	stages	1351:1356	arg1	both					1324:1327	both	1324:1327	both	1324:1327	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	7	83	dep	stages	1351:1356	arg1	maturity					1342:1349	maturity	1342:1349	maturity	1342:1349	In addition, the pectin methylation of pmei12 lines is decreased as expected, and the pectin content of the cell wall increased at both seedling and maturity stages; however, the cellulose and hemicellulose increased only at seedling stage.
36555723	3	84	from	elements	580:587	arg1	region					605:610	the promoter region	592:610	the promoter region	592:610	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	3	85	theme	PMEI	462:465	arg1	genes					467:471	45 PMEI genes	459:471	45 PMEI genes from rice (Oryza sativa L.)	459:499	In this study, 45 PMEI genes from rice (Oryza sativa L.) were screened by bioinformatics tools, and their structure, motifs, cis-acting elements in the promoter region, chromosomal distribution, gene duplication, and phylogenetic relationship were analyzed.
36555723	1	86	theme	primary	200:206	arg1	wall					213:216	the primary cell wall	196:216	the primary cell wall	196:216	Pectin is one of the constituents of the cell wall, distributed in the primary cell wall and middle lamella, affecting the rheological properties and the cell wall stickiness.
36242028	2	0	theme	more	241:244	arg1	history					230:236	a history	228:236	a history of more than 1300 years of application in both medicine and food in China	228:310	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	7	1	theme	Network	647:653	arg1	pharmacology					655:666	GC‒MS. Network pharmacology	640:666	GC‒MS. Network pharmacology	640:666	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	12	2	theme	ethyl	1365:1369	arg1	octanoate					1371:1379	ethyl octanoate	1365:1379	ethyl octanoate	1365:1379	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	4	3	from	additives	398:406	arg1	aromatherapy					431:442	aromatherapy	431:442	aromatherapy	431:442	Plena (PREO) is one of the most frequently used additives in food, cosmetics and aromatherapy.
36242028	4	3	from	additives	398:406	arg1	cosmetics					417:425	cosmetics	417:425	cosmetics	417:425	Plena (PREO) is one of the most frequently used additives in food, cosmetics and aromatherapy.
36242028	4	3	from	additives	398:406	arg1	food					411:414	food	411:414	food	411:414	Plena (PREO) is one of the most frequently used additives in food, cosmetics and aromatherapy.
36242028	1	4	theme	BACKGROUND	163:172	arg1	cv					186:187	BACKGROUND Rosa rugosa cv	163:187	BACKGROUND Rosa rugosa cv.	163:188	BACKGROUND Rosa rugosa cv.
36242028	15	5	theme	pharmacology	1899:1910	arg1	study					1859:1863	The integrated study	1844:1863	The integrated study of molecular analysis and network pharmacology	1844:1910	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	2	6	theme	application	265:275	arg1	1300 years					251:260	1300 years	251:260	1300 years of application in both medicine and food in China	251:310	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	1	7	theme	rugosa	179:184	arg1	cv					186:187	BACKGROUND Rosa rugosa cv	163:187	BACKGROUND Rosa rugosa cv.	163:188	BACKGROUND Rosa rugosa cv.
36242028	9	8	theme	biochemical	927:937	arg1	assays					939:944	biochemical assays	927:944	biochemical assays	927:944	A series of experiments, including biochemical assays, RT‒PCR, and western blotting, were conducted to investigate the anti-inflammatory and antioxidative effects of PREO.
36242028	13	9	theme	possible	1545:1552	arg1	targets					1554:1560	possible targets	1545:1560	possible targets	1545:1560	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	15	10	theme	molecular	1868:1876	arg1	analysis					1878:1885	molecular analysis	1868:1885	molecular analysis	1868:1885	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	0	11	theme	in	119:120	arg1	analysis					153:160	an integrated in vitro and network pharmacology analysis	105:160	an integrated in vitro and network pharmacology analysis	105:160	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	7	12	theme	PREO	617:620	arg1	composition					602:612	The composition	598:612	The composition of PREO	598:620	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	6	13	dep	mechanisms	539:548	arg1	behind					550:555	behind	550:555	behind these effects	550:569	However, the mechanisms behind these effects are still unclear.
36242028	13	14	theme	signaling	1708:1716	arg1	regulation					1688:1697	the regulation	1684:1697	the regulation of NF-κB signaling	1684:1716	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	9	15	theme	western	959:965	arg1	blotting					967:974	western blotting	959:974	western blotting	959:974	A series of experiments, including biochemical assays, RT‒PCR, and western blotting, were conducted to investigate the anti-inflammatory and antioxidative effects of PREO.
36242028	0	16	theme	pharmacology	140:151	arg1	analysis					153:160	an integrated in vitro and network pharmacology analysis	105:160	an integrated in vitro and network pharmacology analysis	105:160	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	7	17	theme	GC‒MS.	640:645	arg1	pharmacology					655:666	GC‒MS. Network pharmacology	640:666	GC‒MS. Network pharmacology	640:666	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	2	18	theme	rugosa	216:221	arg1	Plena					190:194	Plena	190:194	Plena	190:194	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	18	theme	rugosa	216:221	arg1	cultivar					199:206	a cultivar	197:206	a cultivar of Rosa rugosa	197:221	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	0	19	dep	in	119:120	arg1	vitro					122:126	vitro	122:126	vitro	122:126	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	15	20	theme	potential	1943:1951	arg1	PREO					1927:1930	PREO	1927:1930	PREO	1927:1930	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	15	20	theme	potential	1943:1951	arg1	agent					1971:1975	a potential anti-inflammatory agent	1941:1975	a potential anti-inflammatory agent to treat inflammation and oxidative stress	1941:2018	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	10	21	theme	SOD	1200:1202	arg1	ROS					1173:1175	ROS	1173:1175	ROS	1173:1175	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	21	theme	SOD	1200:1202	arg1	MDA					1182:1184	MDA	1182:1184	MDA	1182:1184	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	21	theme	SOD	1200:1202	arg1	NO					1169:1170	NO	1169:1170	NO	1169:1170	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	21	theme	SOD	1200:1202	arg1	activities					1212:1221	preserved SOD and CAT activities	1190:1221	preserved SOD and CAT activities	1190:1221	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	8	22	theme	inflammatory	809:820	arg1	model					834:838	An inflammatory immune cell model	806:838	An inflammatory immune cell model	806:838	An inflammatory immune cell model was constructed by exposing RAW 264.7 cells to LPS.
36242028	12	23	theme	pathway	1479:1485	arg1	proteins					1487:1494	several inflammatory pathway proteins	1458:1494	several inflammatory pathway proteins	1458:1494	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	8	24	theme	cell	829:832	arg1	model					834:838	An inflammatory immune cell model	806:838	An inflammatory immune cell model	806:838	An inflammatory immune cell model was constructed by exposing RAW 264.7 cells to LPS.
36242028	0	25	from	inflammation	50:61	arg1	cells					76:80	RAW 264.7 cells	66:80	RAW 264.7 cells	66:80	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	13	26	theme	RAW	1643:1645	arg1	cells					1653:1657	LPS-treated RAW 264.7 cells	1631:1657	LPS-treated RAW 264.7 cells	1631:1657	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	9	27	theme	anti-inflammatory	1011:1027	arg1	effects					1047:1053	the anti-inflammatory and antioxidative effects	1007:1053	the anti-inflammatory and antioxidative effects of PREO	1007:1061	A series of experiments, including biochemical assays, RT‒PCR, and western blotting, were conducted to investigate the anti-inflammatory and antioxidative effects of PREO.
36242028	13	28	from	responses	1618:1626	arg1	cells					1653:1657	LPS-treated RAW 264.7 cells	1631:1657	LPS-treated RAW 264.7 cells	1631:1657	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	4	29	theme	additives	398:406	arg1	additives					398:406	the most frequently used additives	373:406	the most frequently used additives in food, cosmetics and aromatherapy	373:442	Plena (PREO) is one of the most frequently used additives in food, cosmetics and aromatherapy.
36242028	4	29	theme	additives	398:406	arg1	one					366:368	one	366:368	one	366:368	Plena (PREO) is one of the most frequently used additives in food, cosmetics and aromatherapy.
36242028	9	30	theme	antioxidative	1033:1045	arg1	effects					1047:1053	the anti-inflammatory and antioxidative effects	1007:1053	the anti-inflammatory and antioxidative effects of PREO	1007:1061	A series of experiments, including biochemical assays, RT‒PCR, and western blotting, were conducted to investigate the anti-inflammatory and antioxidative effects of PREO.
36242028	0	31	theme	Pingyin	0:6	arg1	oil					23:25	Pingyin rose essential oil	0:25	Pingyin rose essential oil	0:25	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	7	32	theme	possible	746:753	arg1	targets					755:761	the possible targets	742:761	the possible targets against inflammation and oxidative stress	742:803	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	5	33	theme	alleviating	505:515	arg1	effects					517:523	some anti-inflammation, antioxidant and nerve alleviating effects	459:523	some anti-inflammation, antioxidant and nerve alleviating effects	459:523	PREO exhibits some anti-inflammation, antioxidant and nerve alleviating effects.
36242028	3	34	theme	essential	317:325	arg1	oil					327:329	The essential oil	313:329	The essential oil of Rosa rugosa cv	313:347	The essential oil of Rosa rugosa cv.
36242028	13	35	theme	anti-inflammatory	1600:1616	arg1	responses					1618:1626	PREO anti-inflammatory responses	1595:1626	PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells	1595:1657	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	0	36	theme	essential	13:21	arg1	oil					23:25	Pingyin rose essential oil	0:25	Pingyin rose essential oil	0:25	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	10	37	theme	PREO	1072:1075	arg1	treatment					1077:1085	RESULTS PREO treatment	1064:1085	RESULTS PREO treatment	1064:1085	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	8	38	theme	RAW	868:870	arg1	cells					878:882	RAW 264.7 cells	868:882	RAW 264.7 cells	868:882	An inflammatory immune cell model was constructed by exposing RAW 264.7 cells to LPS.
36242028	12	39	theme	inflammatory	1466:1477	arg1	proteins					1487:1494	several inflammatory pathway proteins	1458:1494	several inflammatory pathway proteins	1458:1494	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	3	40	theme	rugosa	339:344	arg1	cv					346:347	Rosa rugosa cv	334:347	Rosa rugosa cv	334:347	The essential oil of Rosa rugosa cv.
36242028	13	41	theme	western	1577:1583	arg1	blotting					1585:1592	western blotting	1577:1592	western blotting	1577:1592	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	0	42	theme	RAW	66:68	arg1	cells					76:80	RAW 264.7 cells	66:80	RAW 264.7 cells	66:80	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	12	43	theme	geranyl	1382:1388	arg1	acetate					1390:1396	geranyl acetate	1382:1396	geranyl acetate	1382:1396	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	15	44	theme	oxidative	2003:2011	arg1	stress					2013:2018	oxidative stress	2003:2018	oxidative stress	2003:2018	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	7	45	theme	possible	697:704	arg1	network					722:728	the possible compound-target network	693:728	the possible compound-target network	693:728	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	0	46	dep	alleviates	27:36	arg1	analysis					153:160	an integrated in vitro and network pharmacology analysis	105:160	an integrated in vitro and network pharmacology analysis	105:160	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	10	47	theme	CAT	1208:1210	arg1	ROS					1173:1175	ROS	1173:1175	ROS	1173:1175	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	47	theme	CAT	1208:1210	arg1	MDA					1182:1184	MDA	1182:1184	MDA	1182:1184	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	47	theme	CAT	1208:1210	arg1	NO					1169:1170	NO	1169:1170	NO	1169:1170	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	47	theme	CAT	1208:1210	arg1	activities					1212:1221	preserved SOD and CAT activities	1190:1221	preserved SOD and CAT activities	1190:1221	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	14	48	theme	Molecular	1719:1727	arg1	docking					1729:1735	Molecular docking	1719:1735	Molecular docking	1719:1735	Molecular docking showed that PREO components can interact with different proteins involved in the NF-κB pathway.
36242028	15	49	theme	network	1891:1897	arg1	pharmacology					1899:1910	network pharmacology	1891:1910	network pharmacology	1891:1910	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	12	50	theme	methyl	1403:1408	arg1	eugenol					1410:1416	methyl eugenol	1403:1416	methyl eugenol	1403:1416	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	2	51	from	medicine	285:292	arg1	China					306:310	China	306:310	China	306:310	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	51	from	medicine	285:292	arg1	1300 years					251:260	1300 years	251:260	1300 years of application in both medicine and food in China	251:310	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	13	52	theme	targets	1554:1560	arg1	expression					1531:1540	the gene and protein expression	1510:1540	expression	1531:1540	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	2	53	contain	has	224:226	arg2	history					230:236	a history	228:236	a history of more than 1300 years of application in both medicine and food in China	228:310	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	53	contain	has	224:226	arg1	Plena					190:194	Plena	190:194	Plena	190:194	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	53	contain	has	224:226	arg1	cultivar					199:206	a cultivar	197:206	a cultivar of Rosa rugosa	197:221	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	54	from	food	298:301	arg1	China					306:310	China	306:310	China	306:310	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	54	from	food	298:301	arg1	1300 years					251:260	1300 years	251:260	1300 years of application in both medicine and food in China	251:310	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	10	55	dep	significantly	1087:1099	arg1	p < 0.05					1102:1109	p < 0.05	1102:1109	p < 0.05	1102:1109	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	14	56	theme	PREO	1749:1752	arg1	components					1754:1763	PREO components	1749:1763	PREO components	1749:1763	Molecular docking showed that PREO components can interact with different proteins involved in the NF-κB pathway.
36242028	9	57	theme	experiments	904:914	arg1	series					894:899	A series	892:899	A series of experiments, including biochemical assays, RT‒PCR, and western blotting,	892:975	A series of experiments, including biochemical assays, RT‒PCR, and western blotting, were conducted to investigate the anti-inflammatory and antioxidative effects of PREO.
36242028	13	58	theme	protein	1523:1529	arg1	expression					1531:1540	the gene and protein expression	1510:1540	expression	1531:1540	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	1	59	theme	Rosa	174:177	arg1	cv					186:187	BACKGROUND Rosa rugosa cv	163:187	BACKGROUND Rosa rugosa cv.	163:188	BACKGROUND Rosa rugosa cv.
36242028	12	60	theme	Network	1307:1313	arg1	pharmacology					1315:1326	Network pharmacology	1307:1326	Network pharmacology	1307:1326	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	15	61	theme	anti-inflammatory	1953:1969	arg1	PREO					1927:1930	PREO	1927:1930	PREO	1927:1930	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	15	61	theme	anti-inflammatory	1953:1969	arg1	agent					1971:1975	a potential anti-inflammatory agent	1941:1975	a potential anti-inflammatory agent to treat inflammation and oxidative stress	1941:2018	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	15	62	theme	analysis	1878:1885	arg1	study					1859:1863	The integrated study	1844:1863	The integrated study of molecular analysis and network pharmacology	1844:1910	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	0	63	theme	integrated	108:117	arg1	analysis					153:160	an integrated in vitro and network pharmacology analysis	105:160	an integrated in vitro and network pharmacology analysis	105:160	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	7	64	theme	oxidative	788:796	arg1	stress					798:803	oxidative stress	788:803	oxidative stress	788:803	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	15	65	dep	CONCLUSION	1833:1842	arg1	suggested					1912:1920	suggested	1912:1920	suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress	1912:2018	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	2	66	theme	Rosa	211:214	arg1	rugosa					216:221	Rosa rugosa	211:221	Rosa rugosa	211:221	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	13	67	theme	NF-κB	1702:1706	arg1	signaling					1708:1716	NF-κB signaling	1702:1716	NF-κB signaling	1702:1716	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	0	68	theme	network	132:138	arg1	analysis					153:160	an integrated in vitro and network pharmacology analysis	105:160	an integrated in vitro and network pharmacology analysis	105:160	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	13	69	theme	gene	1514:1517	arg1	expression					1531:1540	the gene and protein expression	1510:1540	expression	1531:1540	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	5	70	theme	anti-inflammation	464:480	arg1	effects					517:523	some anti-inflammation, antioxidant and nerve alleviating effects	459:523	some anti-inflammation, antioxidant and nerve alleviating effects	459:523	PREO exhibits some anti-inflammation, antioxidant and nerve alleviating effects.
36242028	5	71	theme	antioxidant	483:493	arg1	effects					517:523	some anti-inflammation, antioxidant and nerve alleviating effects	459:523	some anti-inflammation, antioxidant and nerve alleviating effects	459:523	PREO exhibits some anti-inflammation, antioxidant and nerve alleviating effects.
36242028	14	72	theme	NF-κB	1818:1822	arg1	pathway					1824:1830	the NF-κB pathway	1814:1830	the NF-κB pathway	1814:1830	Molecular docking showed that PREO components can interact with different proteins involved in the NF-κB pathway.
36242028	2	73	from	1300 years	251:260	arg1	food					298:301	food	298:301	food	298:301	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	73	from	1300 years	251:260	arg1	medicine					285:292	medicine	285:292	medicine	285:292	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	7	74	dep	METHODS	590:596	arg1	determined					626:635	determined	626:635	was determined by GC‒MS. Network pharmacology	622:666	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	11	75	theme	GC‒MS	1224:1228	arg1	analysis					1230:1237	GC‒MS analysis	1224:1237	GC‒MS analysis	1224:1237	GC‒MS analysis revealed that PREO consists of 57 compounds, mainly monoterpenoids.
36242028	10	76	theme	preserved	1190:1198	arg1	SOD					1200:1202	preserved SOD	1190:1202	preserved SOD	1190:1202	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	0	77	theme	NF-κB	90:94	arg1	pathway					96:102	the NF-κB pathway	86:102	the NF-κB pathway	86:102	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	2	78	from	application	265:275	arg1	food					298:301	food	298:301	food	298:301	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	2	78	from	application	265:275	arg1	medicine					285:292	medicine	285:292	medicine	285:292	Plena, a cultivar of Rosa rugosa, has a history of more than 1300 years of application in both medicine and food in China.
36242028	4	79	theme	used	393:396	arg1	additives					398:406	the most frequently used additives	373:406	the most frequently used additives in food, cosmetics and aromatherapy	373:442	Plena (PREO) is one of the most frequently used additives in food, cosmetics and aromatherapy.
36242028	8	80	theme	immune	822:827	arg1	model					834:838	An inflammatory immune cell model	806:838	An inflammatory immune cell model	806:838	An inflammatory immune cell model was constructed by exposing RAW 264.7 cells to LPS.
36242028	0	81	theme	rose	8:11	arg1	oil					23:25	Pingyin rose essential oil	0:25	Pingyin rose essential oil	0:25	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	3	82	theme	Rosa	334:337	arg1	cv					346:347	Rosa rugosa cv	334:347	Rosa rugosa cv	334:347	The essential oil of Rosa rugosa cv.
36242028	14	83	theme	different	1783:1791	arg1	proteins					1793:1800	different proteins	1783:1800	different proteins involved in the NF-κB pathway	1783:1830	Molecular docking showed that PREO components can interact with different proteins involved in the NF-κB pathway.
36242028	9	84	theme	PREO	1058:1061	arg1	effects					1047:1053	the anti-inflammatory and antioxidative effects	1007:1053	the anti-inflammatory and antioxidative effects of PREO	1007:1061	A series of experiments, including biochemical assays, RT‒PCR, and western blotting, were conducted to investigate the anti-inflammatory and antioxidative effects of PREO.
36242028	0	85	theme	LPS-Induced	38:48	arg1	inflammation					50:61	LPS-Induced inflammation	38:61	LPS-Induced inflammation in RAW 264.7 cells	38:80	Pingyin rose essential oil alleviates LPS-Induced inflammation in RAW 264.7 cells via the NF-κB pathway: an integrated in vitro and network pharmacology analysis.
36242028	10	86	theme	RESULTS	1064:1070	arg1	treatment					1077:1085	RESULTS PREO treatment	1064:1085	RESULTS PREO treatment	1064:1085	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	13	87	theme	LPS-treated	1631:1641	arg1	cells					1653:1657	LPS-treated RAW 264.7 cells	1631:1657	LPS-treated RAW 264.7 cells	1631:1657	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	12	88	theme	several	1458:1464	arg1	proteins					1487:1494	several inflammatory pathway proteins	1458:1494	several inflammatory pathway proteins	1458:1494	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	3	89	theme	cv	346:347	arg1	oil					327:329	The essential oil	313:329	The essential oil of Rosa rugosa cv	313:347	The essential oil of Rosa rugosa cv.
36242028	7	90	theme	compound-target	706:720	arg1	network					722:728	the possible compound-target network	693:728	the possible compound-target network	693:728	METHODS The composition of PREO was determined by GC‒MS. Network pharmacology was performed to predict the possible compound-target network and analyze the possible targets against inflammation and oxidative stress.
36242028	13	91	theme	PREO	1595:1598	arg1	responses					1618:1626	PREO anti-inflammatory responses	1595:1626	PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells	1595:1657	By measuring the gene and protein expression of possible targets by qRT‒PCR and western blotting, PREO anti-inflammatory responses in LPS-treated RAW 264.7 cells might be associated with the regulation of NF-κB signaling.
36242028	10	92	theme	oxidative	1140:1148	arg1	NO					1169:1170	NO	1169:1170	NO	1169:1170	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	92	theme	oxidative	1140:1148	arg1	ROS					1173:1175	ROS	1173:1175	ROS	1173:1175	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	92	theme	oxidative	1140:1148	arg1	biomarkers					1150:1159	inflammatory and oxidative biomarkers	1123:1159	biomarkers	1150:1159	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	92	theme	oxidative	1140:1148	arg1	MDA					1182:1184	MDA	1182:1184	MDA	1182:1184	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	12	93	theme	active	1423:1428	arg1	components					1430:1439	active components	1423:1439	active components interacting with several inflammatory pathway proteins	1423:1494	Network pharmacology revealed that citronellol, farnesol, ethyl octanoate, geranyl acetate, and methyl eugenol were active components interacting with several inflammatory pathway proteins.
36242028	15	94	theme	integrated	1848:1857	arg1	study					1859:1863	The integrated study	1844:1863	The integrated study of molecular analysis and network pharmacology	1844:1910	CONCLUSION The integrated study of molecular analysis and network pharmacology suggested that PREO might be a potential anti-inflammatory agent to treat inflammation and oxidative stress.
36242028	10	95	theme	inflammatory	1123:1134	arg1	NO					1169:1170	NO	1169:1170	NO	1169:1170	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	95	theme	inflammatory	1123:1134	arg1	ROS					1173:1175	ROS	1173:1175	ROS	1173:1175	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	95	theme	inflammatory	1123:1134	arg1	biomarkers					1150:1159	inflammatory and oxidative biomarkers	1123:1159	biomarkers	1150:1159	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
36242028	10	95	theme	inflammatory	1123:1134	arg1	MDA					1182:1184	MDA	1182:1184	MDA	1182:1184	RESULTS PREO treatment significantly (p < 0.05) alleviated inflammatory and oxidative biomarkers such as NO, ROS, and MDA and preserved SOD and CAT activities.
35460827	7	0	theme	jujube	1230:1235	arg1	polyphenols					1242:1252	jujube peel polyphenols	1230:1252	jujube peel polyphenols	1230:1252	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	8	1	from	inflammation	1455:1466	arg1	264.7 cells					1475:1485	RAW 264.7 cells	1471:1485	RAW 264.7 cells	1471:1485	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	4	2	theme	peel	700:703	arg1	Meanwhile					682:690	Meanwhile	682:690	Meanwhile	682:690	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	4	2	theme	peel	700:703	arg1	polyphenols					705:715	jujube peel polyphenols	693:715	jujube peel polyphenols	693:715	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	1	3	theme	traditional	183:193	arg1	potential					161:169	great potential	155:169	great potential	155:169	Jujube has great potential as food and traditional drugs in several countries.
35460827	1	3	theme	traditional	183:193	arg1	drugs					195:199	traditional drugs	183:199	traditional drugs	183:199	Jujube has great potential as food and traditional drugs in several countries.
35460827	7	4	theme	peel	1237:1240	arg1	polyphenols					1242:1252	jujube peel polyphenols	1230:1252	jujube peel polyphenols	1230:1252	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	8	5	theme	new	1538:1540	arg1	drugs					1560:1564	new anti-inflammatory drugs	1538:1564	new anti-inflammatory drugs	1538:1564	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	4	6	theme	jujube	693:698	arg1	Meanwhile					682:690	Meanwhile	682:690	Meanwhile	682:690	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	4	6	theme	jujube	693:698	arg1	polyphenols					705:715	jujube peel polyphenols	693:715	jujube peel polyphenols	693:715	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	3	7	from	peel	556:559	arg1	composition					519:529	the phenolic composition	506:529	the phenolic composition of polyphenols in jujube peel	506:559	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	4	8	theme	TNF-α	746:750	arg1	generation					732:741	the generation	728:741	the generation of TNF-α, IL-1β, IL-6, NO and PGE2	728:776	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	1	9	contain	has	151:153	arg2	drugs					195:199	traditional drugs	183:199	traditional drugs	183:199	Jujube has great potential as food and traditional drugs in several countries.
35460827	1	9	contain	has	151:153	arg2	potential					161:169	great potential	155:169	great potential	155:169	Jujube has great potential as food and traditional drugs in several countries.
35460827	1	9	contain	has	151:153	arg1	Jujube					144:149	Jujube	144:149	Jujube	144:149	Jujube has great potential as food and traditional drugs in several countries.
35460827	1	10	from	drugs	195:199	arg1	countries					212:220	several countries	204:220	several countries	204:220	Jujube has great potential as food and traditional drugs in several countries.
35460827	2	11	theme	RAW	327:329	arg1	264.7 cells					331:341	RAW 264.7 cells	327:341	RAW 264.7 cells	327:341	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	5	12	theme	oxidative	985:993	arg1	stress					995:1000	oxidative stress	985:1000	oxidative stress	985:1000	Additionally, jujube peel polyphenol activate Nrf2 from the cytoplasm to the nucleus, regulate antioxidant enzymes and pro-inflammatory cytokines, and reduce oxidative stress and inflammatory responses.
35460827	0	13	theme	RAW	127:129	arg1	264.7 cells					131:141	RAW 264.7 cells	127:141	RAW 264.7 cells	127:141	Jujube peel polyphenols synergistically inhibit lipopolysaccharide-induced inflammation through multiple signaling pathways in RAW 264.7 cells.
35460827	8	14	theme	drugs	1560:1564	arg1	development					1523:1533	the development	1519:1533	the development of new anti-inflammatory drugs	1519:1564	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	6	15	theme	Nrf2	1192:1195	arg1	pathways					1207:1214	Nrf2 signaling pathways	1192:1214	Nrf2 signaling pathways	1192:1214	Results obtained from this study suggest that jujube peel polyphenols may alleviate oxidative stress and inflammation by inhibiting MAPK and NF-κB and activating Nrf2 signaling pathways.
35460827	1	16	from	food	174:177	arg1	countries					212:220	several countries	204:220	several countries	204:220	Jujube has great potential as food and traditional drugs in several countries.
35460827	2	17	theme	nuclear	392:398	arg1	NF-κB					416:420	NF-κB	416:420	NF-κB	416:420	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	17	theme	nuclear	392:398	arg1	B					413:413	nuclear factor-kappa B	392:413	nuclear factor-kappa B (NF-κB)	392:421	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	5	18	from	cytoplasm	887:895	arg1	Nrf2					873:876	Nrf2	873:876	Nrf2 from the cytoplasm to the nucleus	873:910	Additionally, jujube peel polyphenol activate Nrf2 from the cytoplasm to the nucleus, regulate antioxidant enzymes and pro-inflammatory cytokines, and reduce oxidative stress and inflammatory responses.
35460827	3	19	from	polyphenols	534:544	arg1	peel					556:559	jujube peel	549:559	jujube peel	549:559	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	4	20	theme	NO	766:767	arg1	generation					732:741	the generation	728:741	the generation of TNF-α, IL-1β, IL-6, NO and PGE2	728:776	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	2	21	from	influence	254:262	arg1	LPS					314:316	LPS	314:316	LPS	314:316	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	21	from	influence	254:262	arg1	lipopolysaccharide					294:311	lipopolysaccharide	294:311	lipopolysaccharide (LPS)	294:317	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	5	22	theme	jujube	841:846	arg1	polyphenol					853:862	jujube peel polyphenol	841:862	jujube peel polyphenol	841:862	Additionally, jujube peel polyphenol activate Nrf2 from the cytoplasm to the nucleus, regulate antioxidant enzymes and pro-inflammatory cytokines, and reduce oxidative stress and inflammatory responses.
35460827	3	23	theme	jujube	549:554	arg1	peel					556:559	jujube peel	549:559	jujube peel	549:559	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	8	24	theme	jujube	1411:1416	arg1	polyphenols					1423:1433	jujube peel polyphenols	1411:1433	jujube peel polyphenols	1411:1433	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	1	25	theme	several	204:210	arg1	countries					212:220	several countries	204:220	several countries	204:220	Jujube has great potential as food and traditional drugs in several countries.
35460827	0	26	theme	peel	7:10	arg1	polyphenols					12:22	Jujube peel polyphenols	0:22	Jujube peel polyphenols	0:22	Jujube peel polyphenols synergistically inhibit lipopolysaccharide-induced inflammation through multiple signaling pathways in RAW 264.7 cells.
35460827	3	27	theme	polyphenols	534:544	arg1	composition					519:529	the phenolic composition	506:529	the phenolic composition of polyphenols in jujube peel	506:559	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	7	28	theme	inflammatory	1312:1323	arg1	treatment					1287:1295	the treatment	1283:1295	the treatment of LPS-induced inflammatory in RAW 264.7 cells	1283:1342	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	7	29	from	treatment	1287:1295	arg1	264.7 cells					1332:1342	RAW 264.7 cells	1328:1342	RAW 264.7 cells	1328:1342	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	2	30	theme	protein	369:375	arg1	MAPK					385:388	MAPK	385:388	MAPK	385:388	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	30	theme	protein	369:375	arg1	kinase					377:382	mitogen-activated protein kinase	351:382	mitogen-activated protein kinase (MAPK)	351:389	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	8	31	theme	anti-inflammatory	1542:1558	arg1	drugs					1560:1564	new anti-inflammatory drugs	1538:1564	new anti-inflammatory drugs	1538:1564	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	0	32	theme	Jujube	0:5	arg1	polyphenols					12:22	Jujube peel polyphenols	0:22	Jujube peel polyphenols	0:22	Jujube peel polyphenols synergistically inhibit lipopolysaccharide-induced inflammation through multiple signaling pathways in RAW 264.7 cells.
35460827	2	33	theme	mitogen-activated	351:367	arg1	MAPK					385:388	MAPK	385:388	MAPK	385:388	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	33	theme	mitogen-activated	351:367	arg1	kinase					377:382	mitogen-activated protein kinase	351:382	mitogen-activated protein kinase (MAPK)	351:389	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	8	34	theme	RAW	1471:1473	arg1	264.7 cells					1475:1485	RAW 264.7 cells	1471:1485	RAW 264.7 cells	1471:1485	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	6	35	theme	jujube	1076:1081	arg1	polyphenols					1088:1098	jujube peel polyphenols	1076:1098	jujube peel polyphenols	1076:1098	Results obtained from this study suggest that jujube peel polyphenols may alleviate oxidative stress and inflammation by inhibiting MAPK and NF-κB and activating Nrf2 signaling pathways.
35460827	8	36	theme	peel	1418:1421	arg1	polyphenols					1423:1433	jujube peel polyphenols	1411:1433	jujube peel polyphenols	1411:1433	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	4	37	theme	IL-1β	753:757	arg1	generation					732:741	the generation	728:741	the generation of TNF-α, IL-1β, IL-6, NO and PGE2	728:776	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	5	38	theme	inflammatory	1006:1017	arg1	responses					1019:1027	inflammatory responses	1006:1027	inflammatory responses	1006:1027	Additionally, jujube peel polyphenol activate Nrf2 from the cytoplasm to the nucleus, regulate antioxidant enzymes and pro-inflammatory cytokines, and reduce oxidative stress and inflammatory responses.
35460827	2	39	theme	factor	455:460	arg1	pathways					481:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	5	40	theme	antioxidant	922:932	arg1	enzymes					934:940	antioxidant enzymes	922:940	antioxidant enzymes	922:940	Additionally, jujube peel polyphenol activate Nrf2 from the cytoplasm to the nucleus, regulate antioxidant enzymes and pro-inflammatory cytokines, and reduce oxidative stress and inflammatory responses.
35460827	6	41	theme	signaling	1197:1205	arg1	pathways					1207:1214	Nrf2 signaling pathways	1192:1214	Nrf2 signaling pathways	1192:1214	Results obtained from this study suggest that jujube peel polyphenols may alleviate oxidative stress and inflammation by inhibiting MAPK and NF-κB and activating Nrf2 signaling pathways.
35460827	7	42	theme	synergistic	1261:1271	arg1	effect					1273:1278	a synergistic effect	1259:1278	a synergistic effect	1259:1278	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	2	43	theme	2-related	445:453	arg1	Nrf2					465:468	Nrf2	465:468	Nrf2	465:468	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	43	theme	2-related	445:453	arg1	factor					455:460	nuclear erythroid 2-related factor 2	427:462	nuclear erythroid 2-related factor 2 (Nrf2)	427:469	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	0	44	theme	lipopolysaccharide-induced	48:73	arg1	inflammation					75:86	lipopolysaccharide-induced inflammation	48:86	lipopolysaccharide-induced inflammation	48:86	Jujube peel polyphenols synergistically inhibit lipopolysaccharide-induced inflammation through multiple signaling pathways in RAW 264.7 cells.
35460827	5	45	theme	pro-inflammatory	946:961	arg1	cytokines					963:971	pro-inflammatory cytokines	946:971	pro-inflammatory cytokines	946:971	Additionally, jujube peel polyphenol activate Nrf2 from the cytoplasm to the nucleus, regulate antioxidant enzymes and pro-inflammatory cytokines, and reduce oxidative stress and inflammatory responses.
35460827	0	46	from	pathways	115:122	arg1	264.7 cells					131:141	RAW 264.7 cells	127:141	RAW 264.7 cells	127:141	Jujube peel polyphenols synergistically inhibit lipopolysaccharide-induced inflammation through multiple signaling pathways in RAW 264.7 cells.
35460827	2	47	theme	peel	274:277	arg1	polyphenols					279:289	jujube peel polyphenols	267:289	jujube peel polyphenols	267:289	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	48	theme	erythroid	435:443	arg1	Nrf2					465:468	Nrf2	465:468	Nrf2	465:468	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	48	theme	erythroid	435:443	arg1	factor					455:460	nuclear erythroid 2-related factor 2	427:462	nuclear erythroid 2-related factor 2 (Nrf2)	427:469	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	6	49	theme	peel	1083:1086	arg1	polyphenols					1088:1098	jujube peel polyphenols	1076:1098	jujube peel polyphenols	1076:1098	Results obtained from this study suggest that jujube peel polyphenols may alleviate oxidative stress and inflammation by inhibiting MAPK and NF-κB and activating Nrf2 signaling pathways.
35460827	2	50	theme	jujube	267:272	arg1	polyphenols					279:289	jujube peel polyphenols	267:289	jujube peel polyphenols	267:289	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	7	51	from	inflammatory	1312:1323	arg1	264.7 cells					1332:1342	RAW 264.7 cells	1328:1342	RAW 264.7 cells	1328:1342	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	2	52	theme	nuclear	427:433	arg1	Nrf2					465:468	Nrf2	465:468	Nrf2	465:468	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	52	theme	nuclear	427:433	arg1	factor					455:460	nuclear erythroid 2-related factor 2	427:462	nuclear erythroid 2-related factor 2 (Nrf2)	427:469	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	3	53	theme	phenolic	510:517	arg1	composition					519:529	the phenolic composition	506:529	the phenolic composition of polyphenols in jujube peel	506:559	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	3	54	theme	p-coumaric	645:654	arg1	acid					656:659	p-coumaric acid	645:659	p-coumaric acid	645:659	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	3	54	theme	p-coumaric	645:654	arg1	rutin					675:679	rutin	675:679	rutin	675:679	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	3	54	theme	p-coumaric	645:654	arg1	catechin					662:669	catechin	662:669	catechin	662:669	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	3	54	theme	p-coumaric	645:654	arg1	polyphenols					628:638	the main polyphenols	619:638	the main polyphenols	619:638	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	4	55	theme	signaling	807:815	arg1	pathways					817:824	NF-κB signaling pathways	801:824	NF-κB signaling pathways	801:824	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	6	56	theme	oxidative	1114:1122	arg1	stress					1124:1129	oxidative stress	1114:1129	oxidative stress	1114:1129	Results obtained from this study suggest that jujube peel polyphenols may alleviate oxidative stress and inflammation by inhibiting MAPK and NF-κB and activating Nrf2 signaling pathways.
35460827	2	57	theme	signaling	471:479	arg1	pathways					481:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	58	theme	anti-inflammatory	236:252	arg1	influence					254:262	the anti-inflammatory influence	232:262	the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS)	232:317	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	4	59	theme	NF-κB	801:805	arg1	pathways					817:824	NF-κB signaling pathways	801:824	NF-κB signaling pathways	801:824	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	1	60	theme	great	155:159	arg1	potential					161:169	great potential	155:169	great potential	155:169	Jujube has great potential as food and traditional drugs in several countries.
35460827	1	60	theme	great	155:159	arg1	drugs					195:199	traditional drugs	183:199	traditional drugs	183:199	Jujube has great potential as food and traditional drugs in several countries.
35460827	8	61	theme	LPS-induced	1443:1453	arg1	inflammation					1455:1466	LPS-induced inflammation	1443:1466	LPS-induced inflammation in RAW 264.7 cells	1443:1485	In conclusion, this study not only reveals the mechanism by which jujube peel polyphenols inhibit LPS-induced inflammation in RAW 264.7 cells, but also provides guidance for the development of new anti-inflammatory drugs.
35460827	4	62	theme	PGE2	773:776	arg1	generation					732:741	the generation	728:741	the generation of TNF-α, IL-1β, IL-6, NO and PGE2	728:776	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	3	63	theme	main	623:626	arg1	acid					656:659	p-coumaric acid	645:659	p-coumaric acid	645:659	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	3	63	theme	main	623:626	arg1	polyphenols					628:638	the main polyphenols	619:638	the main polyphenols	619:638	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	3	64	from	composition	519:529	arg1	peel					556:559	jujube peel	549:559	jujube peel	549:559	In this study, the phenolic composition of polyphenols in jujube peel was analyzed using LC-MS/MS, and which was confirmed that the main polyphenols were p-coumaric acid, catechin and rutin.
35460827	7	65	from	264.7 cells	1332:1342	arg1	treatment					1287:1295	the treatment	1283:1295	the treatment of LPS-induced inflammatory in RAW 264.7 cells	1283:1342	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	7	65	from	264.7 cells	1332:1342	arg1	inflammatory					1312:1323	inflammatory	1312:1323	inflammatory	1312:1323	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	4	66	theme	IL-6	760:763	arg1	generation					732:741	the generation	728:741	the generation of TNF-α, IL-1β, IL-6, NO and PGE2	728:776	Meanwhile, jujube peel polyphenols attenuated the generation of TNF-α, IL-1β, IL-6, NO and PGE2 by inhibiting MAPK and NF-κB signaling pathways.
35460827	2	67	theme	kinase	377:382	arg1	pathways					481:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	0	68	theme	signaling	105:113	arg1	pathways					115:122	multiple signaling pathways	96:122	multiple signaling pathways in RAW 264.7 cells	96:141	Jujube peel polyphenols synergistically inhibit lipopolysaccharide-induced inflammation through multiple signaling pathways in RAW 264.7 cells.
35460827	7	69	theme	RAW	1328:1330	arg1	264.7 cells					1332:1342	RAW 264.7 cells	1328:1342	RAW 264.7 cells	1328:1342	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	2	70	theme	B	413:413	arg1	pathways					481:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways	351:488	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	5	71	theme	peel	848:851	arg1	polyphenol					853:862	jujube peel polyphenol	841:862	jujube peel polyphenol	841:862	Additionally, jujube peel polyphenol activate Nrf2 from the cytoplasm to the nucleus, regulate antioxidant enzymes and pro-inflammatory cytokines, and reduce oxidative stress and inflammatory responses.
35460827	0	72	theme	multiple	96:103	arg1	pathways					115:122	multiple signaling pathways	96:122	multiple signaling pathways in RAW 264.7 cells	96:141	Jujube peel polyphenols synergistically inhibit lipopolysaccharide-induced inflammation through multiple signaling pathways in RAW 264.7 cells.
35460827	2	73	theme	polyphenols	279:289	arg1	influence					254:262	the anti-inflammatory influence	232:262	the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS)	232:317	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	7	74	contain	have	1254:1257	arg1	polyphenols					1242:1252	jujube peel polyphenols	1230:1252	jujube peel polyphenols	1230:1252	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	7	74	contain	have	1254:1257	arg2	effect					1273:1278	a synergistic effect	1259:1278	a synergistic effect	1259:1278	Furthermore, jujube peel polyphenols have a synergistic effect in the treatment of LPS-induced inflammatory in RAW 264.7 cells.
35460827	2	75	theme	factor-kappa	400:411	arg1	NF-κB					416:420	NF-κB	416:420	NF-κB	416:420	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
35460827	2	75	theme	factor-kappa	400:411	arg1	B					413:413	nuclear factor-kappa B	392:413	nuclear factor-kappa B (NF-κB)	392:421	To study the anti-inflammatory influence of jujube peel polyphenols in lipopolysaccharide (LPS) induced RAW 264.7 cells through mitogen-activated protein kinase (MAPK), nuclear factor-kappa B (NF-κB) and nuclear erythroid 2-related factor 2 (Nrf2) signaling pathways.
36064986	6	0	theme	time	985:988	arg1	measure					974:980	a validated composite measure	952:980	a validated composite measure of time to diet tolerance and passage of stool	952:1027	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	6	0	theme	time	985:988	arg1	GI-2					946:949	GI-2	946:949	GI-2	946:949	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	10	1	theme	0-10	1484:1487	arg1	days					1503:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	9	2	theme	median	1388:1393	arg1	years					1410:1414	median age 67 [18-94] years	1388:1414	median age 67 [18-94] years	1388:1414	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	3	theme	[42.4	1228:1232	arg1	%					1233:1233	%	1233:1233	%	1233:1233	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	6	4	theme	validated	954:962	arg1	measure					974:980	a validated composite measure	952:980	a validated composite measure of time to diet tolerance and passage of stool	952:1027	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	6	4	theme	validated	954:962	arg1	GI-2					946:949	GI-2	946:949	GI-2	946:949	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	15	5	theme	reduced	2152:2158	arg1	time					2160:2163	a reduced time	2150:2163	a reduced time to achieving first stool and GI-2	2150:2197	CONCLUSIONS Patients who received sugammadex had a reduced time to achieving first stool and GI-2.
36064986	14	6	theme	postoperative	2028:2040	arg1	p < 0.05					2054:2061	p < 0.05	2054:2061	p < 0.05	2054:2061	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	14	6	theme	postoperative	2028:2040	arg1	use					2049:2051	increased postoperative opioid use	2018:2051	increased postoperative opioid use (p < 0.05)	2018:2062	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	4	7	theme	neuromuscular	655:667	arg1	agents					678:683	neuromuscular reversal agents	655:683	neuromuscular reversal agents used during anaesthesia	655:707	The aim of our study was to assess the impact of neuromuscular reversal agents used during anaesthesia on gastrointestinal recovery.
36064986	9	8	theme	age	1252:1254	arg1	years					1267:1271	median age 64 [19-90] years	1245:1271	median age 64 [19-90] years	1245:1271	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	2	9	theme	acetylcholinesterase	317:336	arg1	Neostigmine					304:314	Neostigmine	304:314	Neostigmine (acetylcholinesterase inhibitor)	304:347	Neostigmine (acetylcholinesterase inhibitor), co-administered with glycopyrrolate, is frequently given for neuromuscular reversal before tracheal extubation and modulates the CAIP.
36064986	2	9	theme	acetylcholinesterase	317:336	arg1	inhibitor					338:346	acetylcholinesterase inhibitor	317:346	acetylcholinesterase inhibitor	317:346	Neostigmine (acetylcholinesterase inhibitor), co-administered with glycopyrrolate, is frequently given for neuromuscular reversal before tracheal extubation and modulates the CAIP.
36064986	12	10	theme	opioid	1786:1791	arg1	use					1793:1795	increased opioid use	1776:1795	increased opioid use	1776:1795	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	15	11	theme	first	2178:2182	arg1	stool					2184:2188	stool	2184:2188	stool	2184:2188	CONCLUSIONS Patients who received sugammadex had a reduced time to achieving first stool and GI-2.
36064986	10	12	theme	first	1541:1545	arg1	stool					1547:1551	first stool	1541:1551	first stool	1541:1551	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	4	13	theme	agents	678:683	arg1	impact					645:650	the impact	641:650	the impact of neuromuscular reversal agents used during anaesthesia on gastrointestinal recovery	641:736	The aim of our study was to assess the impact of neuromuscular reversal agents used during anaesthesia on gastrointestinal recovery.
36064986	14	14	theme	bowel	1996:2000	arg1	anastomoses					2002:2012	bowel anastomoses	1996:2012	bowel anastomoses	1996:2012	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	9	15	theme	[19-90	1259:1264	arg1	years					1267:1271	median age 64 [19-90] years	1245:1271	median age 64 [19-90] years	1245:1271	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	10	16	dep	achieved	1438:1445	arg1	days					1503:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	7	17	theme	stay	1079:1082	arg1	complications					1051:1063	30-day complications	1044:1063	30-day complications	1044:1063	Demographics, 30-day complications and length of stay were collected.
36064986	7	17	theme	stay	1079:1082	arg1	length					1069:1074	length	1069:1074	length of stay	1069:1082	Demographics, 30-day complications and length of stay were collected.
36064986	7	17	theme	stay	1079:1082	arg1	Demographics					1030:1041	Demographics	1030:1041	Demographics	1030:1041	Demographics, 30-day complications and length of stay were collected.
36064986	6	18	theme	diet	993:996	arg1	tolerance					998:1006	diet tolerance	993:1006	diet tolerance	993:1006	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	11	19	dep	length	1633:1638	arg1	stay					1643:1646	stay	1643:1646	stay	1643:1646	Rates of POI, complications and length of stay were similar.
36064986	1	20	theme	common	178:183	arg1	complication					185:196	a common complication	176:196	a common complication	176:196	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	1	20	theme	common	178:183	arg1	BACKGROUND					136:145	BACKGROUND	136:145	BACKGROUND Postoperative ileus (POI)	136:171	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	0	21	from	Effect	0:5	arg1	ileus					100:104	postoperative ileus	86:104	postoperative ileus	86:104	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.
36064986	14	22	theme	multivariate	1960:1971	arg1	analysis					1973:1980	multivariate analysis	1960:1980	multivariate analysis	1960:1980	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	16	23	theme	Neostigmine	2200:2210	arg1	use					2212:2214	Neostigmine use	2200:2214	Neostigmine use	2200:2214	Neostigmine use, bowel anastomoses and postoperative opioid use were associated with delayed time to achieving GI-2.
36064986	11	24	theme	POI	1610:1612	arg1	Rates					1601:1605	Rates	1601:1605	Rates of POI	1601:1612	Rates of POI, complications and length of stay were similar.
36064986	11	24	theme	POI	1610:1612	arg1	length					1633:1638	length	1633:1638	length of stay	1633:1646	Rates of POI, complications and length of stay were similar.
36064986	11	24	theme	POI	1610:1612	arg1	complications					1615:1627	complications	1615:1627	complications	1615:1627	Rates of POI, complications and length of stay were similar.
36064986	0	25	theme	colorectal	116:125	arg1	surgery					127:133	colorectal surgery	116:133	colorectal surgery	116:133	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.
36064986	8	26	theme	Univariate	1100:1109	arg1	analyses					1128:1135	Univariate and multivariate analyses	1100:1135	Univariate and multivariate analyses	1100:1135	Univariate and multivariate analyses were performed.
36064986	2	27	theme	tracheal	441:448	arg1	extubation					450:459	tracheal extubation	441:459	tracheal extubation	441:459	Neostigmine (acetylcholinesterase inhibitor), co-administered with glycopyrrolate, is frequently given for neuromuscular reversal before tracheal extubation and modulates the CAIP.
36064986	13	28	theme	anastomotic	1899:1909	arg1	leak					1911:1914	anastomotic leak	1899:1914	anastomotic leak	1899:1914	POI was associated with increased complications, including anastomotic leak and prolonged hospital stay (p < 0.001).
36064986	8	29	theme	multivariate	1115:1126	arg1	analyses					1128:1135	Univariate and multivariate analyses	1100:1135	Univariate and multivariate analyses	1100:1135	Univariate and multivariate analyses were performed.
36064986	9	30	dep	males	1358:1362	arg1	years					1410:1414	median age 67 [18-94] years	1388:1414	median age 67 [18-94] years	1388:1414	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	10	31	theme	Sugammadex	1418:1427	arg1	patients					1429:1436	Sugammadex patients	1418:1436	Sugammadex patients	1418:1436	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	0	32	theme	postoperative	86:98	arg1	ileus					100:104	postoperative ileus	86:104	postoperative ileus	86:104	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.
36064986	12	33	theme	abdominal	1736:1744	arg1	surgery					1746:1752	previous abdominal surgery	1727:1752	previous abdominal surgery	1727:1752	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	0	34	theme	neuromuscular	10:22	arg1	reversal					24:31	neuromuscular reversal	10:31	neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex	10:81	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.
36064986	9	35	theme	[55.9	1350:1354	arg1	%					1355:1355	%	1355:1355	%	1355:1355	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	3	36	theme	reversal	500:507	arg1	agent					509:513	An alternative reversal agent	485:513	An alternative reversal agent	485:513	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	3	36	theme	reversal	500:507	arg1	sugammadex					516:525	sugammadex	516:525	sugammadex (selective rocuronium or vecuronium binder)	516:569	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	12	37	theme	smoking	1710:1716	arg1	history					1718:1724	smoking history	1710:1724	smoking history	1710:1724	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	12	38	theme	univariate	1665:1674	arg1	analysis					1676:1683	univariate analysis	1665:1683	univariate analysis	1665:1683	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	10	39	theme	median	1474:1479	arg1	days					1503:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	16	40	theme	opioid	2253:2258	arg1	use					2260:2262	postoperative opioid use	2239:2262	postoperative opioid use	2239:2262	Neostigmine use, bowel anastomoses and postoperative opioid use were associated with delayed time to achieving GI-2.
36064986	10	41	theme	3	1574:1574	arg1	days					1583:1586	2 (0-10) vs. 3 (0-12) days	1561:1586	median 2 (0-10) vs. 3 (0-12) days	1554:1586	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	9	42	theme	[44.1	1371:1375	arg1	%					1376:1376	%	1376:1376	%	1376:1376	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	16	43	theme	postoperative	2239:2251	arg1	use					2260:2262	postoperative opioid use	2239:2262	postoperative opioid use	2239:2262	Neostigmine use, bowel anastomoses and postoperative opioid use were associated with delayed time to achieving GI-2.
36064986	1	44	dep	BACKGROUND	136:145	arg1	POI					168:170	POI	168:170	POI	168:170	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	1	44	dep	BACKGROUND	136:145	arg1	ileus					161:165	Postoperative ileus	147:165	BACKGROUND Postoperative ileus (POI)	136:171	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	10	45	theme	3	1494:1494	arg1	days					1503:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	median 3 (0-10) vs. 3 (0-12) days	1474:1506	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	10	46	dep	median	1554:1559	arg1	days					1583:1586	2 (0-10) vs. 3 (0-12) days	1561:1586	median 2 (0-10) vs. 3 (0-12) days	1554:1586	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	10	46	dep	median	1554:1559	arg1	p = 0.035					1589:1597	p = 0.035	1589:1597	p = 0.035	1589:1597	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	9	47	theme	%	1212:1212	arg1	patients					1193:1200	twenty-four (66.9%) patients	1173:1200	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years)	1153:1272	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	47	theme	%	1212:1212	arg1	males					1215:1219	129 [57.6%] males	1203:1219	129 [57.6%] males	1203:1219	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	3	48	dep	sugammadex	516:525	arg1	rocuronium					538:547	selective rocuronium	528:547	selective rocuronium	528:547	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	3	48	dep	sugammadex	516:525	arg1	binder					563:568	vecuronium binder	552:568	vecuronium binder	552:568	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	10	49	dep	reduced	1525:1531	arg1	median					1554:1559	median	1554:1559	median 2 (0-10) vs. 3 (0-12) days	1554:1586	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	3	50	theme	vecuronium	552:561	arg1	binder					563:568	vecuronium binder	552:568	vecuronium binder	552:568	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	1	51	theme	anti-inflammatory	270:286	arg1	pathway					288:294	the cholinergic anti-inflammatory pathway	254:294	the cholinergic anti-inflammatory pathway (CAIP)	254:301	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	1	51	theme	anti-inflammatory	270:286	arg1	CAIP					297:300	CAIP	297:300	CAIP	297:300	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	9	52	theme	95	1225:1226	arg1	%					1233:1233	%	1233:1233	%	1233:1233	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	53	theme	age	1395:1397	arg1	years					1410:1414	median age 67 [18-94] years	1388:1414	median age 67 [18-94] years	1388:1414	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	6	54	theme	composite	964:972	arg1	measure					974:980	a validated composite measure	952:980	a validated composite measure of time to diet tolerance and passage of stool	952:1027	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	6	54	theme	composite	964:972	arg1	GI-2					946:949	GI-2	946:949	GI-2	946:949	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	4	55	theme	study	621:625	arg1	aim					610:612	The aim	606:612	The aim of our study	606:625	The aim of our study was to assess the impact of neuromuscular reversal agents used during anaesthesia on gastrointestinal recovery.
36064986	9	56	theme	%	1233:1233	arg1	females					1236:1242	95 [42.4%] females	1225:1242	95 [42.4%] females	1225:1242	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	56	theme	%	1233:1233	arg1	patients					1193:1200	twenty-four (66.9%) patients	1173:1200	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years)	1153:1272	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	57	theme	[18-94	1402:1407	arg1	years					1410:1414	median age 67 [18-94] years	1388:1414	median age 67 [18-94] years	1388:1414	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	14	58	theme	opioid	2042:2047	arg1	p < 0.05					2054:2061	p < 0.05	2054:2061	p < 0.05	2054:2061	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	14	58	theme	opioid	2042:2047	arg1	use					2049:2051	increased postoperative opioid use	2018:2051	increased postoperative opioid use (p < 0.05)	2018:2062	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	1	59	theme	colorectal	208:217	arg1	surgery					219:225	colorectal surgery	208:225	colorectal surgery	208:225	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	12	60	theme	postoperative	1801:1813	arg1	hypokalaemia					1815:1826	postoperative hypokalaemia	1801:1826	postoperative hypokalaemia (p < 0.05)	1801:1837	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	12	60	theme	postoperative	1801:1813	arg1	p < 0.05					1829:1836	p < 0.05	1829:1836	p < 0.05	1829:1836	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	14	61	theme	increased	2018:2026	arg1	p < 0.05					2054:2061	p < 0.05	2054:2061	p < 0.05	2054:2061	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	14	61	theme	increased	2018:2026	arg1	use					2049:2051	increased postoperative opioid use	2018:2051	increased postoperative opioid use (p < 0.05)	2018:2062	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	9	62	theme	median	1245:1250	arg1	years					1267:1271	median age 64 [19-90] years	1245:1271	median age 64 [19-90] years	1245:1271	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	6	63	theme	stool	1023:1027	arg1	passage					1012:1018	passage	1012:1018	passage of stool	1012:1027	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	6	63	theme	stool	1023:1027	arg1	tolerance					998:1006	diet tolerance	993:1006	diet tolerance	993:1006	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	10	64	dep	days	1503:1506	arg1	p = 0.036					1509:1517	p = 0.036	1509:1517	p = 0.036	1509:1517	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	12	65	theme	increased	1776:1784	arg1	use					1793:1795	increased opioid use	1776:1795	increased opioid use	1776:1795	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	15	66	contain	had	2146:2148	arg2	time					2160:2163	a reduced time	2150:2163	a reduced time to achieving first stool and GI-2	2150:2197	CONCLUSIONS Patients who received sugammadex had a reduced time to achieving first stool and GI-2.
36064986	15	66	contain	had	2146:2148	arg1	Patients					2113:2120	CONCLUSIONS Patients	2101:2120	CONCLUSIONS Patients who received sugammadex	2101:2144	CONCLUSIONS Patients who received sugammadex had a reduced time to achieving first stool and GI-2.
36064986	4	67	theme	reversal	669:676	arg1	agents					678:683	neuromuscular reversal agents	655:683	neuromuscular reversal agents used during anaesthesia	655:707	The aim of our study was to assess the impact of neuromuscular reversal agents used during anaesthesia on gastrointestinal recovery.
36064986	1	68	theme	Postoperative	147:159	arg1	POI					168:170	POI	168:170	POI	168:170	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	1	68	theme	Postoperative	147:159	arg1	ileus					161:165	Postoperative ileus	147:165	BACKGROUND Postoperative ileus (POI)	136:171	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	16	69	theme	bowel	2217:2221	arg1	anastomoses					2223:2233	bowel anastomoses	2217:2233	bowel anastomoses	2217:2233	Neostigmine use, bowel anastomoses and postoperative opioid use were associated with delayed time to achieving GI-2.
36064986	4	70	from	impact	645:650	arg1	recovery					729:736	gastrointestinal recovery	712:736	gastrointestinal recovery	712:736	The aim of our study was to assess the impact of neuromuscular reversal agents used during anaesthesia on gastrointestinal recovery.
36064986	4	71	theme	gastrointestinal	712:727	arg1	recovery					729:736	gastrointestinal recovery	712:736	gastrointestinal recovery	712:736	The aim of our study was to assess the impact of neuromuscular reversal agents used during anaesthesia on gastrointestinal recovery.
36064986	5	72	theme	elective	793:800	arg1	surgery					813:819	elective colorectal surgery	793:819	elective colorectal surgery	793:819	METHODS Three hundred thirty-five patients undergoing elective colorectal surgery at the Royal Adelaide Hospital between January 2019 and December 2021 were retrospectively included.
36064986	13	73	theme	prolonged	1920:1928	arg1	hospital					1930:1937	prolonged hospital	1920:1937	prolonged hospital stay (p < 0.001)	1920:1954	POI was associated with increased complications, including anastomotic leak and prolonged hospital stay (p < 0.001).
36064986	13	73	theme	prolonged	1920:1928	arg1	p < 0.001					1945:1953	p < 0.001	1945:1953	p < 0.001	1945:1953	POI was associated with increased complications, including anastomotic leak and prolonged hospital stay (p < 0.001).
36064986	12	74	theme	colostomy	1755:1763	arg1	formation					1765:1773	colostomy formation	1755:1773	colostomy formation	1755:1773	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	7	75	theme	30-day	1044:1049	arg1	complications					1051:1063	30-day complications	1044:1063	30-day complications	1044:1063	Demographics, 30-day complications and length of stay were collected.
36064986	5	76	dep	METHODS	739:745	arg1	patients					773:780	Three hundred thirty-five patients	747:780	METHODS Three hundred thirty-five patients undergoing elective colorectal surgery at the Royal Adelaide Hospital between January 2019 and December 2021	739:889	METHODS Three hundred thirty-five patients undergoing elective colorectal surgery at the Royal Adelaide Hospital between January 2019 and December 2021 were retrospectively included.
36064986	5	77	theme	colorectal	802:811	arg1	surgery					813:819	elective colorectal surgery	793:819	elective colorectal surgery	793:819	METHODS Three hundred thirty-five patients undergoing elective colorectal surgery at the Royal Adelaide Hospital between January 2019 and December 2021 were retrospectively included.
36064986	12	78	theme	previous	1727:1734	arg1	surgery					1746:1752	previous abdominal surgery	1727:1752	previous abdominal surgery	1727:1752	On univariate analysis, POI was associated with smoking history, previous abdominal surgery, colostomy formation, increased opioid use and postoperative hypokalaemia (p < 0.05).
36064986	3	79	theme	alternative	488:498	arg1	agent					509:513	An alternative reversal agent	485:513	An alternative reversal agent	485:513	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	3	79	theme	alternative	488:498	arg1	sugammadex					516:525	sugammadex	516:525	sugammadex (selective rocuronium or vecuronium binder)	516:569	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	14	80	theme	time	2087:2090	arg1	predictive					2073:2082	predictive	2073:2082	predictive	2073:2082	On multivariate analysis, neostigmine, bowel anastomoses and increased postoperative opioid use (p < 0.05) remained predictive of time to GI-2.
36064986	5	81	theme	thirty-five	761:771	arg1	patients					773:780	Three hundred thirty-five patients	747:780	METHODS Three hundred thirty-five patients undergoing elective colorectal surgery at the Royal Adelaide Hospital between January 2019 and December 2021	739:889	METHODS Three hundred thirty-five patients undergoing elective colorectal surgery at the Royal Adelaide Hospital between January 2019 and December 2021 were retrospectively included.
36064986	13	82	dep	hospital	1930:1937	arg1	stay					1939:1942	stay	1939:1942	stay	1939:1942	POI was associated with increased complications, including anastomotic leak and prolonged hospital stay (p < 0.001).
36064986	2	83	theme	neuromuscular	411:423	arg1	reversal					425:432	neuromuscular reversal	411:432	neuromuscular reversal	411:432	Neostigmine (acetylcholinesterase inhibitor), co-administered with glycopyrrolate, is frequently given for neuromuscular reversal before tracheal extubation and modulates the CAIP.
36064986	0	84	theme	reversal	24:31	arg1	Effect					0:5	Effect	0:5	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus	0:104	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.
36064986	9	85	dep	males	1215:1219	arg1	years					1267:1271	median age 64 [19-90] years	1245:1271	median age 64 [19-90] years	1245:1271	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	86	theme	%	1355:1355	arg1	males					1358:1362	62 [55.9%] males	1347:1362	62 [55.9%] males	1347:1362	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	87	dep	received	1326:1333	arg1	males					1358:1362	62 [55.9%] males	1347:1362	62 [55.9%] males	1347:1362	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	87	dep	received	1326:1333	arg1	females					1379:1385	49 [44.1%] females	1368:1385	49 [44.1%] females	1368:1385	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	10	88	theme	GI-2	1447:1450	arg1	sooner					1452:1457	GI-2 sooner	1447:1457	GI-2 sooner	1447:1457	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	0	89	with	reversal	24:31	arg1	neostigmine/glycopyrrolate					38:63	neostigmine/glycopyrrolate	38:63	neostigmine/glycopyrrolate	38:63	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.
36064986	0	89	with	reversal	24:31	arg1	sugammadex					72:81	sugammadex	72:81	sugammadex	72:81	Effect of neuromuscular reversal with neostigmine/glycopyrrolate versus sugammadex on postoperative ileus following colorectal surgery.
36064986	10	90	theme	2	1561:1561	arg1	days					1583:1586	2 (0-10) vs. 3 (0-12) days	1561:1586	median 2 (0-10) vs. 3 (0-12) days	1554:1586	Sugammadex patients achieved GI-2 sooner after surgery (median 3 (0-10) vs. 3 (0-12) days, p = 0.036), and reduced time to first stool (median 2 (0-10) vs. 3 (0-12) days, p = 0.035).
36064986	15	91	theme	CONCLUSIONS	2101:2111	arg1	Patients					2113:2120	CONCLUSIONS Patients	2101:2120	CONCLUSIONS Patients who received sugammadex	2101:2144	CONCLUSIONS Patients who received sugammadex had a reduced time to achieving first stool and GI-2.
36064986	6	92	theme	primary	926:932	arg1	outcome					934:940	The primary outcome	922:940	The primary outcome	922:940	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	6	92	theme	primary	926:932	arg1	GI-2					946:949	GI-2	946:949	GI-2	946:949	The primary outcome was GI-2, a validated composite measure of time to diet tolerance and passage of stool.
36064986	13	93	theme	increased	1864:1872	arg1	leak					1911:1914	anastomotic leak	1899:1914	anastomotic leak	1899:1914	POI was associated with increased complications, including anastomotic leak and prolonged hospital stay (p < 0.001).
36064986	13	93	theme	increased	1864:1872	arg1	hospital					1930:1937	prolonged hospital	1920:1937	prolonged hospital stay (p < 0.001)	1920:1954	POI was associated with increased complications, including anastomotic leak and prolonged hospital stay (p < 0.001).
36064986	13	93	theme	increased	1864:1872	arg1	complications					1874:1886	increased complications	1864:1886	increased complications	1864:1886	POI was associated with increased complications, including anastomotic leak and prolonged hospital stay (p < 0.001).
36064986	9	94	dep	RESULTS	1153:1159	arg1	females					1236:1242	95 [42.4%] females	1225:1242	95 [42.4%] females	1225:1242	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	94	dep	RESULTS	1153:1159	arg1	males					1215:1219	129 [57.6%] males	1203:1219	129 [57.6%] males	1203:1219	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	94	dep	RESULTS	1153:1159	arg1	patients					1193:1200	twenty-four (66.9%) patients	1173:1200	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years)	1153:1272	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	94	dep	RESULTS	1153:1159	arg1	%					1190:1190	66.9%	1186:1190	66.9%	1186:1190	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	9	95	theme	[57.6	1207:1211	arg1	%					1212:1212	%	1212:1212	%	1212:1212	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	3	96	theme	selective	528:536	arg1	rocuronium					538:547	selective rocuronium	528:547	selective rocuronium	528:547	An alternative reversal agent, sugammadex (selective rocuronium or vecuronium binder), acts independently from the CAIP.
36064986	9	97	theme	%	1376:1376	arg1	females					1379:1385	49 [44.1%] females	1368:1385	49 [44.1%] females	1368:1385	RESULTS Two hundred twenty-four (66.9%) patients (129 [57.6%] males and 95 [42.4%] females, median age 64 [19-90] years) received neostigmine/glycopyrrolate and 111 (33.1%) received sugammadex (62 [55.9%] males and 49 [44.1%] females, median age 67 [18-94] years).
36064986	15	98	theme	achieving	2168:2176	arg1	stool					2184:2188	stool	2184:2188	stool	2184:2188	CONCLUSIONS Patients who received sugammadex had a reduced time to achieving first stool and GI-2.
36064986	16	99	theme	delayed	2285:2291	arg1	time					2293:2296	delayed time	2285:2296	delayed time to achieving GI-2	2285:2314	Neostigmine use, bowel anastomoses and postoperative opioid use were associated with delayed time to achieving GI-2.
36064986	1	100	theme	cholinergic	258:268	arg1	pathway					288:294	the cholinergic anti-inflammatory pathway	254:294	the cholinergic anti-inflammatory pathway (CAIP)	254:301	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36064986	1	100	theme	cholinergic	258:268	arg1	CAIP					297:300	CAIP	297:300	CAIP	297:300	BACKGROUND Postoperative ileus (POI) is a common complication following colorectal surgery and is mediated in part by the cholinergic anti-inflammatory pathway (CAIP).
36356716	7	0	theme	fluorescence	1191:1202	arg1	FISH					1227:1230	FISH	1227:1230	FISH	1227:1230	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	7	0	theme	fluorescence	1191:1202	arg1	hybridisation					1212:1224	fluorescence in situ hybridisation	1191:1224	fluorescence in situ hybridisation (FISH)	1191:1231	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	10	1	theme	ELISA	1830:1834	arg1	kit					1836:1838	an ELISA kit	1827:1838	an ELISA kit	1827:1838	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	15	2	theme	IL-22+	2736:2741	arg1	ILC3					2743:2746	IL-22+ ILC3	2736:2746	IL-22+ ILC3	2736:2746	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	12	3	theme	colon	2279:2283	arg1	injury					2298:2303	colon pathological injury	2279:2303	colon pathological injury	2279:2303	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	1	4	theme	febrile	280:286	arg1	diseases					288:295	febrile diseases	280:295	febrile diseases	280:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	11	5	theme	tryptophan	1863:1872	arg1	metabolomics					1874:1885	targeted tryptophan metabolomics	1854:1885	targeted tryptophan metabolomics	1854:1885	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	3	6	theme	tryptophan	536:545	arg1	metabolism					547:556	gut microbiota-related tryptophan metabolism	513:556	gut microbiota-related tryptophan metabolism	513:556	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	16	7	theme	receptor	2975:2982	arg1	antagonist					2990:2999	aryl hydrocarbon receptor (AhR) antagonist	2958:2999	aryl hydrocarbon receptor (AhR) antagonist	2958:2999	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	1	8	used	used	315:318	arg2	medicine					246:253	a traditional Chinese medicine	224:253	a traditional Chinese medicine derived from Treatise on febrile diseases	224:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	8	used	used	315:318	arg2	RELEVANCE					181:189	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	11	9	theme	gut	2061:2063	arg1	metabolism					2095:2104	gut microbiota-related tryptophan metabolism	2061:2104	gut microbiota-related tryptophan metabolism	2061:2104	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	6	10	theme	microplate	1114:1123	arg1	analyser					1125:1132	a fluorescein microplate analyser	1100:1132	a fluorescein microplate analyser	1100:1132	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	18	11	theme	indole	3415:3420	arg1	derivatives					3422:3432	indole derivatives	3415:3432	indole derivatives	3415:3432	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	3	12	theme	gut	513:515	arg1	metabolism					547:556	gut microbiota-related tryptophan metabolism	513:556	gut microbiota-related tryptophan metabolism	513:556	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	5	13	theme	activity	820:827	arg1	DAI					836:838	DAI	836:838	DAI	836:838	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	5	13	theme	activity	820:827	arg1	index					829:833	disease activity index	812:833	disease activity index (DAI)	812:839	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	15	14	theme	protein	2796:2802	arg1	proportion					2722:2731	the proportion	2718:2731	the proportion of IL-22+ ILC3 in the lamina propria	2718:2768	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	14	theme	protein	2796:2802	arg1	level					2829:2833	the level	2825:2833	the level of IL-22 in colon homogenates	2825:2863	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	14	theme	protein	2796:2802	arg1	expression					2775:2784	the expression	2771:2784	the expression of CYP1A1 protein in colon tissue	2771:2818	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	15	theme	lamina	2755:2760	arg1	propria					2762:2768	the lamina propria	2751:2768	the lamina propria	2751:2768	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	18	16	from	barrier	3305:3311	arg1	mice					3319:3322	UC mice	3316:3322	UC mice	3316:3322	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	13	17	theme	bacterial	2439:2447	arg1	infiltration					2449:2460	bacterial infiltration	2439:2460	bacterial infiltration in MLNs and colon tissues	2439:2486	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	3	18	theme	protective	571:580	arg1	effects					582:588	protective effects	571:588	protective effects of GQD against intestinal barrier damage	571:629	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	16	19	theme	antagonist	2990:2999	arg1	co-administration					2929:2945	co-administration	2929:2945	co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist	2929:2999	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	18	20	theme	damaged	3286:3292	arg1	barrier					3305:3311	the damaged intestinal barrier	3282:3311	the damaged intestinal barrier in UC mice	3282:3322	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	18	21	theme	AhR-mediated	3446:3457	arg1	production					3465:3474	AhR-mediated IL-22 production	3446:3474	AhR-mediated IL-22 production	3446:3474	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	2	22	theme	GQD	408:410	arg1	treatment					412:420	GQD treatment	408:420	GQD treatment	408:420	However, the potential mechanism of GQD treatment for UC remains elusive.
36356716	17	23	theme	gut	3130:3132	arg1	microbiota					3134:3143	the gut microbiota	3126:3143	the gut microbiota	3126:3143	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	0	24	theme	ulcerative	135:144	arg1	colitis					146:152	ulcerative colitis	135:152	ulcerative colitis mice	135:157	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	10	25	theme	IL-22+	1710:1715	arg1	cells					1723:1727	interleukin-22-positive (IL-22+) ILC3 cells	1685:1727	interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes	1685:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	5	26	theme	body	799:802	arg1	weight					804:809	body weight	799:809	body weight	799:809	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	14	27	theme	goblet	2553:2558	arg1	cells					2560:2564	colonic goblet cells	2545:2564	colonic goblet cells	2545:2564	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	7	28	from	infiltration	1145:1156	arg1	tissues					1167:1173	colon tissues	1161:1173	colon tissues	1161:1173	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	14	29	theme	junction	2658:2665	arg1	proteins					2667:2674	tight junction proteins	2652:2674	tight junction proteins	2652:2674	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	10	30	theme	propria	1739:1745	arg1	lymphocytes					1747:1757	lamina propria lymphocytes	1732:1757	lamina propria lymphocytes	1732:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	5	31	from	weight	804:809	arg1	colon					894:898	the colon	890:898	the colon	890:898	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	1	32	theme	Chinese	238:244	arg1	RELEVANCE					181:189	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	32	theme	Chinese	238:244	arg1	medicine					246:253	a traditional Chinese medicine	224:253	a traditional Chinese medicine derived from Treatise on febrile diseases	224:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	8	33	theme	Alcian	1393:1398	arg1	staining					1405:1412	Alcian blue staining	1393:1412	Alcian blue staining	1393:1412	Subsequently, colonic goblet cells were detected using Alcian blue staining.
36356716	4	34	theme	sodium	709:714	arg1	DSS					717:719	3% dextran sulfate sodium (DSS)	690:720	3% dextran sulfate sodium (DSS)	690:720	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	1	35	from	Treatise	268:275	arg1	diseases					288:295	febrile diseases	280:295	febrile diseases	280:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	0	36	from	metabolism	121:130	arg1	mice					154:157	ulcerative colitis mice	135:157	ulcerative colitis mice	135:157	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	13	37	from	diffusion	2346:2354	arg1	serum					2428:2432	serum	2428:2432	serum	2428:2432	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	37	from	diffusion	2346:2354	arg1	MLNs					2465:2468	MLNs	2465:2468	MLNs	2465:2468	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	37	from	diffusion	2346:2354	arg1	tract					2390:2394	the intestinal tract	2375:2394	the intestinal tract	2375:2394	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	37	from	diffusion	2346:2354	arg1	tissues					2480:2486	colon tissues	2474:2486	colon tissues	2474:2486	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	11	38	theme	derivatives	1934:1944	arg1	concentration					1910:1922	the concentration	1906:1922	the concentration of indole derivatives produced by tryptophan metabolism in faeces	1906:1988	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	5	39	from	index	829:833	arg1	colon					894:898	the colon	890:898	the colon	890:898	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	9	40	theme	colonic	1442:1448	arg1	epithelium					1450:1459	the colonic epithelium	1438:1459	the colonic epithelium	1438:1459	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	18	41	theme	tryptophan	3361:3370	arg1	metabolism					3372:3381	gut microbiota-related tryptophan metabolism	3338:3381	gut microbiota-related tryptophan metabolism	3338:3381	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	12	42	theme	colon	2257:2261	arg1	shortening					2263:2272	colon shortening	2257:2272	colon shortening	2257:2272	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	14	43	theme	tight	2652:2656	arg1	proteins					2667:2674	tight junction proteins	2652:2674	tight junction proteins	2652:2674	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	4	44	theme	3	690:690	arg1	%					691:691	%	691:691	%	691:691	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	17	45	theme	tryptophan	3076:3085	arg1	metabolism					3087:3096	tryptophan metabolism	3076:3096	tryptophan metabolism	3076:3096	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	1	46	theme	ulcerative	347:356	arg1	UC					367:368	UC	367:368	UC	367:368	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	46	theme	ulcerative	347:356	arg1	colitis					358:364	acute ulcerative colitis	341:364	acute ulcerative colitis (UC)	341:369	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	3	47	theme	barrier	616:622	arg1	damage					624:629	intestinal barrier damage	605:629	intestinal barrier damage	605:629	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	18	48	theme	gut	3338:3340	arg1	metabolism					3372:3381	gut microbiota-related tryptophan metabolism	3338:3381	gut microbiota-related tryptophan metabolism	3338:3381	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	4	49	theme	dextran	693:699	arg1	DSS					717:719	3% dextran sulfate sodium (DSS)	690:720	3% dextran sulfate sodium (DSS)	690:720	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	1	50	theme	Qinlian	197:203	arg1	GQD					216:218	GQD	216:218	GQD	216:218	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	50	theme	Qinlian	197:203	arg1	decoction					205:213	Gegen Qinlian decoction	191:213	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	10	51	from	cells	1723:1727	arg1	lymphocytes					1747:1757	lamina propria lymphocytes	1732:1757	lamina propria lymphocytes	1732:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	6	52	theme	isothiocyanate	933:946	arg1	FITC-dextran					957:968	FITC-dextran	957:968	FITC-dextran	957:968	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	6	52	theme	isothiocyanate	933:946	arg1	dextran					948:954	fluorescein isothiocyanate dextran	921:954	fluorescein isothiocyanate dextran (FITC-dextran)	921:969	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	12	53	theme	pathological	2166:2177	arg1	symptoms					2179:2186	the pathological symptoms	2162:2186	the pathological symptoms	2162:2186	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	53	theme	pathological	2166:2177	arg1	loss					2206:2209	weight loss	2199:2209	weight loss	2199:2209	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	0	54	theme	microbiota-related	91:108	arg1	metabolism					121:130	gut microbiota-related tryptophan metabolism	87:130	gut microbiota-related tryptophan metabolism in ulcerative colitis mice	87:157	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	10	55	theme	interleukin-22-positive	1685:1707	arg1	cells					1723:1727	interleukin-22-positive (IL-22+) ILC3 cells	1685:1727	interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes	1685:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	9	56	theme	electron	1494:1501	arg1	microscopy					1503:1512	transmission electron microscopy	1481:1512	transmission electron microscopy	1481:1512	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	12	57	theme	UC	2308:2309	arg1	mice					2311:2314	UC mice	2308:2314	UC mice	2308:2314	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	15	58	from	expression	2775:2784	arg1	tissue					2813:2818	colon tissue	2807:2818	colon tissue	2807:2818	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	58	from	expression	2775:2784	arg1	homogenates					2853:2863	colon homogenates	2847:2863	colon homogenates	2847:2863	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	58	from	expression	2775:2784	arg1	propria					2762:2768	the lamina propria	2751:2768	the lamina propria	2751:2768	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	5	59	from	length	855:860	arg1	colon					894:898	the colon	890:898	the colon	890:898	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	10	60	from	lymphocytes	1747:1757	arg1	proportion					1671:1680	the proportion	1667:1680	the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes	1667:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	6	61	theme	small	1014:1018	arg1	imaging					1027:1033	small animal imaging	1014:1033	small animal imaging	1014:1033	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	12	62	theme	DAI	2222:2224	arg1	score					2226:2230	increased DAI score	2212:2230	increased DAI score	2212:2230	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	62	theme	DAI	2222:2224	arg1	loss					2206:2209	weight loss	2199:2209	weight loss	2199:2209	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	17	63	theme	genes	3191:3195	arg1	abundance					3178:3186	the abundance	3174:3186	the abundance of genes related to tryptophan metabolism	3174:3228	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	2	64	theme	potential	385:393	arg1	mechanism					395:403	the potential mechanism	381:403	the potential mechanism of GQD treatment for UC	381:427	However, the potential mechanism of GQD treatment for UC remains elusive.
36356716	0	65	theme	Gegen	0:4	arg1	decoction					14:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	5	66	from	index	848:852	arg1	colon					894:898	the colon	890:898	the colon	890:898	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	7	67	dep	in	1204:1205	arg1	situ					1207:1210	situ	1207:1210	situ	1207:1210	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	7	68	theme	mesenteric	1260:1269	arg1	MLNs					1284:1287	MLNs	1284:1287	MLNs	1284:1287	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	7	68	theme	mesenteric	1260:1269	arg1	nodes					1277:1281	mesenteric lymph nodes	1260:1281	mesenteric lymph nodes (MLNs)	1260:1288	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	11	69	theme	tryptophan	2084:2093	arg1	metabolism					2095:2104	gut microbiota-related tryptophan metabolism	2061:2104	gut microbiota-related tryptophan metabolism	2061:2104	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	10	70	used	used	1651:1654	arg2	cytometry					1637:1645	flow cytometry	1632:1645	flow cytometry	1632:1645	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	15	71	theme	colon	2807:2811	arg1	tissue					2813:2818	colon tissue	2807:2818	colon tissue	2807:2818	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	10	72	from	content	1768:1774	arg1	homogenates					1794:1804	colon homogenates	1788:1804	colon homogenates	1788:1804	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	9	73	theme	epithelium	1450:1459	arg1	junctions					1425:1433	The tight junctions	1415:1433	The tight junctions of the colonic epithelium	1415:1459	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	6	74	from	distribution	905:916	arg1	tract					989:993	the intestinal tract	974:993	the intestinal tract	974:993	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	2	75	theme	treatment	412:420	arg1	mechanism					395:403	the potential mechanism	381:403	the potential mechanism of GQD treatment for UC	381:427	However, the potential mechanism of GQD treatment for UC remains elusive.
36356716	5	76	theme	GQD	761:763	arg1	effects					750:756	The therapeutic effects	734:756	The therapeutic effects of GQD in UC mice	734:774	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	16	77	theme	protective	2885:2894	arg1	effects					2896:2902	the above protective effects	2875:2902	the above protective effects of GQD	2875:2909	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	9	78	theme	junction	1543:1550	arg1	proteins					1552:1559	tight junction proteins	1537:1559	tight junction proteins	1537:1559	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	18	79	theme	UC	3316:3317	arg1	mice					3319:3322	UC mice	3316:3322	UC mice	3316:3322	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	15	80	from	proportion	2722:2731	arg1	tissue					2813:2818	colon tissue	2807:2818	colon tissue	2807:2818	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	80	from	proportion	2722:2731	arg1	homogenates					2853:2863	colon homogenates	2847:2863	colon homogenates	2847:2863	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	80	from	proportion	2722:2731	arg1	propria					2762:2768	the lamina propria	2751:2768	the lamina propria	2751:2768	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	18	81	theme	microbiota-related	3342:3359	arg1	metabolism					3372:3381	gut microbiota-related tryptophan metabolism	3338:3381	gut microbiota-related tryptophan metabolism	3338:3381	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	5	82	theme	UC	768:769	arg1	mice					771:774	UC mice	768:774	UC mice	768:774	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	4	83	dep	MATERIALS	632:640	arg1	Mice					654:657	Mice	654:657	Mice	654:657	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	4	84	with	METHODS	646:652	arg1	colitis					664:670	colitis	664:670	colitis	664:670	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	8	85	theme	goblet	1360:1365	arg1	cells					1367:1371	colonic goblet cells	1352:1371	colonic goblet cells	1352:1371	Subsequently, colonic goblet cells were detected using Alcian blue staining.
36356716	10	86	theme	flow	1632:1635	arg1	cytometry					1637:1645	flow cytometry	1632:1645	flow cytometry	1632:1645	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	11	87	theme	indole	1927:1932	arg1	derivatives					1934:1944	indole derivatives	1927:1944	indole derivatives produced by tryptophan metabolism in faeces	1927:1988	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	14	88	theme	tight	2604:2608	arg1	junctions					2610:2618	epithelial tight junctions	2593:2618	epithelial tight junctions	2593:2618	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	11	89	from	metabolism	1969:1978	arg1	faeces					1983:1988	faeces	1983:1988	faeces	1983:1988	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	4	90	with	MATERIALS	632:640	arg1	colitis					664:670	colitis	664:670	colitis	664:670	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	5	91	from	changes	879:885	arg1	colon					894:898	the colon	890:898	the colon	890:898	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	16	92	theme	GQD	2907:2909	arg1	effects					2896:2902	the above protective effects	2875:2902	the above protective effects of GQD	2875:2909	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	5	93	theme	therapeutic	738:748	arg1	effects					750:756	The therapeutic effects	734:756	The therapeutic effects of GQD in UC mice	734:774	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	18	94	dep	CONCLUSION	3231:3240	arg1	confirmed					3254:3262	confirmed	3254:3262	confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production	3254:3474	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	7	95	theme	in	1204:1205	arg1	FISH					1227:1230	FISH	1227:1230	FISH	1227:1230	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	7	95	theme	in	1204:1205	arg1	hybridisation					1212:1224	fluorescence in situ hybridisation	1191:1224	fluorescence in situ hybridisation (FISH)	1191:1231	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	3	96	theme	microbiota-related	517:534	arg1	metabolism					547:556	gut microbiota-related tryptophan metabolism	513:556	gut microbiota-related tryptophan metabolism	513:556	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	9	97	theme	western	1605:1611	arg1	blot					1613:1616	western blot	1605:1616	western blot	1605:1616	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	18	98	theme	derivatives	3422:3432	arg1	generation					3401:3410	the generation	3397:3410	the generation of indole derivatives to activate AhR-mediated IL-22 production	3397:3474	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	11	99	used	used	2008:2011	arg2	rDNA					1999:2002	16S rDNA	1995:2002	16S rDNA	1995:2002	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	16	100	theme	hydrocarbon	2963:2973	arg1	AhR					2985:2987	AhR	2985:2987	AhR	2985:2987	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	16	100	theme	hydrocarbon	2963:2973	arg1	receptor					2975:2982	aryl hydrocarbon receptor	2958:2982	aryl hydrocarbon receptor (AhR) antagonist	2958:2999	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	5	101	theme	disease	812:818	arg1	DAI					836:838	DAI	836:838	DAI	836:838	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	5	101	theme	disease	812:818	arg1	index					829:833	disease activity index	812:833	disease activity index (DAI)	812:839	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	0	102	theme	repair	47:52	arg1	barrier					65:71	repair intestinal barrier	47:71	repair intestinal barrier	47:71	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	11	103	theme	microbiota-related	2065:2082	arg1	metabolism					2095:2104	gut microbiota-related tryptophan metabolism	2061:2104	gut microbiota-related tryptophan metabolism	2061:2104	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	6	104	theme	fluorescein	1102:1112	arg1	analyser					1125:1132	a fluorescein microplate analyser	1100:1132	a fluorescein microplate analyser	1100:1132	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	10	105	theme	cells	1723:1727	arg1	proportion					1671:1680	the proportion	1667:1680	the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes	1667:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	18	106	theme	IL-22	3459:3463	arg1	production					3465:3474	AhR-mediated IL-22 production	3446:3474	AhR-mediated IL-22 production	3446:3474	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	11	107	dep	composition	2032:2042	arg1	the					2028:2030	the	2028:2030	the	2028:2030	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	0	108	theme	colitis	146:152	arg1	mice					154:157	ulcerative colitis mice	135:157	ulcerative colitis mice	135:157	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	3	109	theme	metabolism	547:556	arg1	involvement					498:508	the involvement	494:508	the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage	494:629	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	10	110	theme	ILC3	1718:1721	arg1	cells					1723:1727	interleukin-22-positive (IL-22+) ILC3 cells	1685:1727	interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes	1685:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	14	111	theme	cells	2560:2564	arg1	number					2535:2540	the number	2531:2540	the number of colonic goblet cells	2531:2564	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	11	112	theme	16S	1995:1997	arg1	rDNA					1999:2002	16S rDNA	1995:2002	16S rDNA	1995:2002	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	5	113	theme	organ	842:846	arg1	index					848:852	organ index	842:852	organ index	842:852	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	17	114	theme	microbiota	3134:3143	arg1	diversity					3113:3121	the diversity	3109:3121	the diversity of the gut microbiota	3109:3143	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	13	115	theme	FITC-dextran	2412:2423	arg1	diffusion					2346:2354	the diffusion	2342:2354	the diffusion of FITC-dextran in the intestinal tract	2342:2394	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	115	theme	FITC-dextran	2412:2423	arg1	content					2401:2407	the content	2397:2407	the content of FITC-dextran in serum	2397:2432	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	115	theme	FITC-dextran	2412:2423	arg1	infiltration					2449:2460	bacterial infiltration	2439:2460	bacterial infiltration in MLNs and colon tissues	2439:2486	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	3	116	theme	GQD	593:595	arg1	effects					582:588	protective effects	571:588	protective effects of GQD against intestinal barrier damage	571:629	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	1	117	theme	traditional	226:236	arg1	RELEVANCE					181:189	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	117	theme	traditional	226:236	arg1	medicine					246:253	a traditional Chinese medicine	224:253	a traditional Chinese medicine derived from Treatise on febrile diseases	224:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	14	118	theme	colonic	2545:2551	arg1	cells					2560:2564	colonic goblet cells	2545:2564	colonic goblet cells	2545:2564	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	8	119	theme	blue	1400:1403	arg1	staining					1405:1412	Alcian blue staining	1393:1412	Alcian blue staining	1393:1412	Subsequently, colonic goblet cells were detected using Alcian blue staining.
36356716	7	120	theme	bacterial	1319:1327	arg1	culture					1329:1335	bacterial culture	1319:1335	bacterial culture	1319:1335	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	12	121	from	changes	2233:2239	arg1	index					2250:2254	organ index	2244:2254	organ index	2244:2254	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	121	from	changes	2233:2239	arg1	mice					2311:2314	UC mice	2308:2314	UC mice	2308:2314	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	121	from	changes	2233:2239	arg1	shortening					2263:2272	colon shortening	2257:2272	colon shortening	2257:2272	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	121	from	changes	2233:2239	arg1	injury					2298:2303	colon pathological injury	2279:2303	colon pathological injury	2279:2303	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	16	122	theme	GQD	2950:2952	arg1	co-administration					2929:2945	co-administration	2929:2945	co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist	2929:2999	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	13	123	theme	intestinal	2379:2388	arg1	tract					2390:2394	the intestinal tract	2375:2394	the intestinal tract	2375:2394	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	10	124	theme	lamina	1732:1737	arg1	lymphocytes					1747:1757	lamina propria lymphocytes	1732:1757	lamina propria lymphocytes	1732:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	13	125	from	infiltration	2449:2460	arg1	serum					2428:2432	serum	2428:2432	serum	2428:2432	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	125	from	infiltration	2449:2460	arg1	MLNs					2465:2468	MLNs	2465:2468	MLNs	2465:2468	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	125	from	infiltration	2449:2460	arg1	tissues					2480:2486	colon tissues	2474:2486	colon tissues	2474:2486	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	125	from	infiltration	2449:2460	arg1	tract					2390:2394	the intestinal tract	2375:2394	the intestinal tract	2375:2394	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	15	126	theme	colon	2847:2851	arg1	homogenates					2853:2863	colon homogenates	2847:2863	colon homogenates	2847:2863	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	4	127	theme	sulfate	701:707	arg1	DSS					717:719	3% dextran sulfate sodium (DSS)	690:720	3% dextran sulfate sodium (DSS)	690:720	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	15	128	theme	ILC3	2743:2746	arg1	proportion					2722:2731	the proportion	2718:2731	the proportion of IL-22+ ILC3 in the lamina propria	2718:2768	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	128	theme	ILC3	2743:2746	arg1	level					2829:2833	the level	2825:2833	the level of IL-22 in colon homogenates	2825:2863	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	128	theme	ILC3	2743:2746	arg1	expression					2775:2784	the expression	2771:2784	the expression of CYP1A1 protein in colon tissue	2771:2818	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	5	129	from	effects	750:756	arg1	mice					771:774	UC mice	768:774	UC mice	768:774	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	10	130	from	proportion	1671:1680	arg1	lymphocytes					1747:1757	lamina propria lymphocytes	1732:1757	lamina propria lymphocytes	1732:1757	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	15	131	theme	IL-22	2838:2842	arg1	proportion					2722:2731	the proportion	2718:2731	the proportion of IL-22+ ILC3 in the lamina propria	2718:2768	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	131	theme	IL-22	2838:2842	arg1	level					2829:2833	the level	2825:2833	the level of IL-22 in colon homogenates	2825:2863	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	131	theme	IL-22	2838:2842	arg1	expression					2775:2784	the expression	2771:2784	the expression of CYP1A1 protein in colon tissue	2771:2818	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	14	132	theme	junctions	2610:2618	arg1	structure					2580:2588	the structure	2576:2588	the structure of epithelial tight junctions	2576:2618	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	3	133	theme	intestinal	605:614	arg1	damage					624:629	intestinal barrier damage	605:629	intestinal barrier damage	605:629	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	1	134	theme	ETHNOPHARMACOLOGICAL	160:179	arg1	RELEVANCE					181:189	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	134	theme	ETHNOPHARMACOLOGICAL	160:179	arg1	medicine					246:253	a traditional Chinese medicine	224:253	a traditional Chinese medicine derived from Treatise on febrile diseases	224:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	13	135	from	content	2401:2407	arg1	serum					2428:2432	serum	2428:2432	serum	2428:2432	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	135	from	content	2401:2407	arg1	MLNs					2465:2468	MLNs	2465:2468	MLNs	2465:2468	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	135	from	content	2401:2407	arg1	tract					2390:2394	the intestinal tract	2375:2394	the intestinal tract	2375:2394	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	135	from	content	2401:2407	arg1	tissues					2480:2486	colon tissues	2474:2486	colon tissues	2474:2486	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	7	136	theme	colon	1161:1165	arg1	tissues					1167:1173	colon tissues	1161:1173	colon tissues	1161:1173	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	0	137	theme	gut	87:89	arg1	metabolism					121:130	gut microbiota-related tryptophan metabolism	87:130	gut microbiota-related tryptophan metabolism in ulcerative colitis mice	87:157	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	1	138	theme	colitis	358:364	arg1	treatment					328:336	the treatment	324:336	the treatment of acute ulcerative colitis (UC)	324:369	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	139	theme	Gegen	191:195	arg1	GQD					216:218	GQD	216:218	GQD	216:218	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	139	theme	Gegen	191:195	arg1	decoction					205:213	Gegen Qinlian decoction	191:213	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	6	140	theme	dextran	948:954	arg1	distribution					905:916	The distribution	901:916	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract	901:993	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	12	141	theme	organ	2244:2248	arg1	index					2250:2254	organ index	2244:2254	organ index	2244:2254	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	4	142	theme	%	691:691	arg1	DSS					717:719	3% dextran sulfate sodium (DSS)	690:720	3% dextran sulfate sodium (DSS)	690:720	MATERIALS AND METHODS Mice with colitis were treated with 3% dextran sulfate sodium (DSS) for 6 days.
36356716	0	143	theme	tryptophan	110:119	arg1	metabolism					121:130	gut microbiota-related tryptophan metabolism	87:130	gut microbiota-related tryptophan metabolism in ulcerative colitis mice	87:157	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	9	144	theme	transmission	1481:1492	arg1	microscopy					1503:1512	transmission electron microscopy	1481:1512	transmission electron microscopy	1481:1512	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	11	145	theme	tryptophan	1958:1967	arg1	metabolism					1969:1978	tryptophan metabolism	1958:1978	tryptophan metabolism in faeces	1958:1988	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	6	146	theme	fluorescein	921:931	arg1	FITC-dextran					957:968	FITC-dextran	957:968	FITC-dextran	957:968	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	6	146	theme	fluorescein	921:931	arg1	dextran					948:954	fluorescein isothiocyanate dextran	921:954	fluorescein isothiocyanate dextran (FITC-dextran)	921:969	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	12	147	theme	increased	2212:2220	arg1	score					2226:2230	increased DAI score	2212:2230	increased DAI score	2212:2230	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	147	theme	increased	2212:2220	arg1	loss					2206:2209	weight loss	2199:2209	weight loss	2199:2209	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	13	148	theme	FITC-dextran	2359:2370	arg1	diffusion					2346:2354	the diffusion	2342:2354	the diffusion of FITC-dextran in the intestinal tract	2342:2394	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	148	theme	FITC-dextran	2359:2370	arg1	content					2401:2407	the content	2397:2407	the content of FITC-dextran in serum	2397:2432	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	13	148	theme	FITC-dextran	2359:2370	arg1	infiltration					2449:2460	bacterial infiltration	2439:2460	bacterial infiltration in MLNs and colon tissues	2439:2486	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	7	149	theme	Bacterial	1135:1143	arg1	infiltration					1145:1156	Bacterial infiltration	1135:1156	Bacterial infiltration in colon tissues	1135:1173	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	15	150	from	level	2829:2833	arg1	tissue					2813:2818	colon tissue	2807:2818	colon tissue	2807:2818	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	150	from	level	2829:2833	arg1	homogenates					2853:2863	colon homogenates	2847:2863	colon homogenates	2847:2863	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	15	150	from	level	2829:2833	arg1	propria					2762:2768	the lamina propria	2751:2768	the lamina propria	2751:2768	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	7	151	theme	lymph	1271:1275	arg1	MLNs					1284:1287	MLNs	1284:1287	MLNs	1284:1287	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	7	151	theme	lymph	1271:1275	arg1	nodes					1277:1281	mesenteric lymph nodes	1260:1281	mesenteric lymph nodes (MLNs)	1260:1288	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	12	152	theme	weight	2199:2204	arg1	changes					2233:2239	changes	2233:2239	changes in organ index, colon shortening, and colon pathological injury in UC mice	2233:2314	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	152	theme	weight	2199:2204	arg1	score					2226:2230	increased DAI score	2212:2230	increased DAI score	2212:2230	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	152	theme	weight	2199:2204	arg1	loss					2206:2209	weight loss	2199:2209	weight loss	2199:2209	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	16	153	theme	aryl	2958:2961	arg1	AhR					2985:2987	AhR	2985:2987	AhR	2985:2987	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	16	153	theme	aryl	2958:2961	arg1	receptor					2975:2982	aryl hydrocarbon receptor	2958:2982	aryl hydrocarbon receptor (AhR) antagonist	2958:2999	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	0	154	theme	Qinlian	6:12	arg1	decoction					14:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction	0:22	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	5	155	theme	pathological	866:877	arg1	changes					879:885	pathological changes	866:885	pathological changes in the colon	866:898	The therapeutic effects of GQD in UC mice were examined based on body weight, disease activity index (DAI), organ index, length and pathological changes in the colon.
36356716	14	156	theme	proteins	2667:2674	arg1	expression					2638:2647	the expression	2634:2647	the expression of tight junction proteins	2634:2674	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	18	157	theme	intestinal	3294:3303	arg1	barrier					3305:3311	the damaged intestinal barrier	3282:3311	the damaged intestinal barrier in UC mice	3282:3322	CONCLUSION Our results confirmed that GQD repaired the damaged intestinal barrier in UC mice by regulating gut microbiota-related tryptophan metabolism and restoring the generation of indole derivatives to activate AhR-mediated IL-22 production.
36356716	17	158	theme	derivatives	3051:3061	arg1	content					3033:3039	the content	3029:3039	the content of indole derivatives generated by tryptophan metabolism	3029:3096	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	17	159	theme	related	3197:3203	arg1	genes					3191:3195	genes	3191:3195	genes related to tryptophan metabolism	3191:3228	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	16	160	theme	above	2879:2883	arg1	effects					2896:2902	the above protective effects	2875:2902	the above protective effects of GQD	2875:2909	However, the above protective effects of GQD were inhibited by co-administration of GQD and aryl hydrocarbon receptor (AhR) antagonist.
36356716	1	161	attach	derived	255:261	arg2	medicine					246:253	a traditional Chinese medicine	224:253	a traditional Chinese medicine derived from Treatise on febrile diseases	224:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	161	attach	derived	255:261	arg2	RELEVANCE					181:189	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	161	attach	derived	255:261	arg1	Treatise					268:275	Treatise	268:275	Treatise on febrile diseases	268:295	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	7	162	theme	bacterial	1242:1250	arg1	load					1252:1255	the bacterial load	1238:1255	the bacterial load in mesenteric lymph nodes (MLNs)	1238:1288	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	11	163	theme	metabolism	2095:2104	arg1	composition					2032:2042	composition	2032:2042	composition	2032:2042	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	11	163	theme	metabolism	2095:2104	arg1	abundance					2048:2056	abundance	2048:2056	abundance	2048:2056	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	10	164	theme	colon	1788:1792	arg1	homogenates					1794:1804	colon homogenates	1788:1804	colon homogenates	1788:1804	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	1	165	dep	RELEVANCE	181:189	arg1	GQD					216:218	GQD	216:218	GQD	216:218	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	165	dep	RELEVANCE	181:189	arg1	decoction					205:213	Gegen Qinlian decoction	191:213	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD)	160:219	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	17	166	theme	tryptophan	3208:3217	arg1	metabolism					3219:3228	tryptophan metabolism	3208:3228	tryptophan metabolism	3208:3228	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	11	167	theme	targeted	1854:1861	arg1	metabolomics					1874:1885	targeted tryptophan metabolomics	1854:1885	targeted tryptophan metabolomics	1854:1885	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	13	168	theme	colon	2474:2478	arg1	tissues					2480:2486	colon tissues	2474:2486	colon tissues	2474:2486	In addition, GQD reduced the diffusion of FITC-dextran in the intestinal tract, the content of FITC-dextran in serum, and bacterial infiltration in MLNs and colon tissues.
36356716	9	169	theme	tight	1537:1541	arg1	proteins					1552:1559	tight junction proteins	1537:1559	tight junction proteins	1537:1559	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	6	170	theme	intestinal	978:987	arg1	tract					989:993	the intestinal tract	974:993	the intestinal tract	974:993	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	6	171	theme	animal	1020:1025	arg1	imaging					1027:1033	small animal imaging	1014:1033	small animal imaging	1014:1033	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	10	172	theme	IL-22	1779:1783	arg1	content					1768:1774	the content	1764:1774	the content of IL-22 in colon homogenates	1764:1804	In addition, flow cytometry was used to analyse the proportion of interleukin-22-positive (IL-22+) ILC3 cells in lamina propria lymphocytes, and the content of IL-22 in colon homogenates was determined using an ELISA kit.
36356716	12	173	dep	RESULTS	2107:2113	arg1	alleviated					2151:2160	alleviated	2151:2160	alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice	2151:2314	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	3	174	theme	STUDY	453:457	arg1	AIM					446:448	AIM	446:448	AIM OF STUDY	446:457	AIM OF STUDY In this study, we aimed to explore the involvement of gut microbiota-related tryptophan metabolism in mediating protective effects of GQD against intestinal barrier damage.
36356716	0	175	theme	intestinal	54:63	arg1	barrier					65:71	repair intestinal barrier	47:71	repair intestinal barrier	47:71	Gegen Qinlian decoction activates AhR/IL-22 to repair intestinal barrier by modulating gut microbiota-related tryptophan metabolism in ulcerative colitis mice.
36356716	9	176	theme	proteins	1552:1559	arg1	expression					1523:1532	the expression	1519:1532	the expression of tight junction proteins	1519:1559	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	15	177	theme	CYP1A1	2789:2794	arg1	protein					2796:2802	CYP1A1 protein	2789:2802	CYP1A1 protein	2789:2802	Furthermore, GQD significantly increased the proportion of IL-22+ ILC3 in the lamina propria, the expression of CYP1A1 protein in colon tissue, and the level of IL-22 in colon homogenates.
36356716	12	178	theme	GQD	2133:2135	arg1	Administration					2115:2128	Administration	2115:2128	Administration of GQD	2115:2135	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	12	179	theme	pathological	2285:2296	arg1	injury					2298:2303	colon pathological injury	2279:2303	colon pathological injury	2279:2303	RESULTS Administration of GQD significantly alleviated the pathological symptoms, including weight loss, increased DAI score, changes in organ index, colon shortening, and colon pathological injury in UC mice.
36356716	1	180	theme	acute	341:345	arg1	UC					367:368	UC	367:368	UC	367:368	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	1	180	theme	acute	341:345	arg1	colitis					358:364	acute ulcerative colitis	341:364	acute ulcerative colitis (UC)	341:369	ETHNOPHARMACOLOGICAL RELEVANCE Gegen Qinlian decoction (GQD) is a traditional Chinese medicine derived from Treatise on febrile diseases and is clinically used for the treatment of acute ulcerative colitis (UC).
36356716	7	181	from	load	1252:1255	arg1	MLNs					1284:1287	MLNs	1284:1287	MLNs	1284:1287	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	7	181	from	load	1252:1255	arg1	nodes					1277:1281	mesenteric lymph nodes	1260:1281	mesenteric lymph nodes (MLNs)	1260:1288	Bacterial infiltration in colon tissues was observed by fluorescence in situ hybridisation (FISH), and the bacterial load in mesenteric lymph nodes (MLNs) was further examined through bacterial culture.
36356716	6	182	theme	FITC-dextran	1059:1070	arg1	concentration					1042:1054	concentration	1042:1054	concentration of FITC-dextran in serum	1042:1079	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
36356716	17	183	theme	indole	3044:3049	arg1	derivatives					3051:3061	indole derivatives	3044:3061	indole derivatives generated by tryptophan metabolism	3044:3096	Additionally, GQD restored the content of indole derivatives generated by tryptophan metabolism, regulated the diversity of the gut microbiota, and significantly increased the abundance of genes related to tryptophan metabolism.
36356716	8	184	theme	colonic	1352:1358	arg1	cells					1367:1371	colonic goblet cells	1352:1371	colonic goblet cells	1352:1371	Subsequently, colonic goblet cells were detected using Alcian blue staining.
36356716	11	185	used	used	1891:1894	arg2	metabolomics					1874:1885	targeted tryptophan metabolomics	1854:1885	targeted tryptophan metabolomics	1854:1885	In addition, targeted tryptophan metabolomics was used to detect the concentration of indole derivatives produced by tryptophan metabolism in faeces, and 16S rDNA was used to investigate the composition and abundance of gut microbiota-related tryptophan metabolism.
36356716	9	186	theme	tight	1419:1423	arg1	junctions					1425:1433	The tight junctions	1415:1433	The tight junctions of the colonic epithelium	1415:1459	The tight junctions of the colonic epithelium were observed using transmission electron microscopy, and the expression of tight junction proteins was detected by immunofluorescence (IF) and western blot.
36356716	14	187	theme	epithelial	2593:2602	arg1	junctions					2610:2618	epithelial tight junctions	2593:2618	epithelial tight junctions	2593:2618	Additionally, GQD significantly increased the number of colonic goblet cells, repaired the structure of epithelial tight junctions and increased the expression of tight junction proteins.
36356716	6	188	from	concentration	1042:1054	arg1	serum					1075:1079	serum	1075:1079	serum	1075:1079	The distribution of fluorescein isothiocyanate dextran (FITC-dextran) in the intestinal tract was observed using small animal imaging, while concentration of FITC-dextran in serum was detected using a fluorescein microplate analyser.
35936076	1	0	theme	elata	269:273	arg1	varieties					253:261	four varieties	248:261	four varieties of G. elata	248:273	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	1	0	theme	elata	269:273	arg1	elata					269:273	G. elata	266:273	G. elata	266:273	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	10	1	theme	higher	1258:1263	arg1	xylose					1265:1270	higher xylose	1258:1270	higher xylose	1258:1270	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	9	2	theme	chemical	1102:1109	arg1	composition					1111:1121	chemical composition	1102:1121	chemical composition	1102:1121	However, GaE-B and GaE-Hyb were found with similar physical properties, chemical composition, and antioxidant and hypoglycemic activity.
35936076	6	3	theme	f.	590:591	arg1	polysaccharides					608:622	f. viridis Makino polysaccharides	590:622	f. viridis Makino polysaccharides	590:622	f. viridis Makino polysaccharides).
35936076	7	4	dep	found	683:687	arg1	whereas					760:766	whereas	760:766	whereas	760:766	As revealed by the results, the GaE polysaccharides were found with the same monosaccharide composition, primarily including glucose, whereas the content of each variety was significantly different.
35936076	12	5	theme	elata	1630:1634	arg1	polysaccharide					1609:1622	the polysaccharide	1605:1622	the polysaccharide of G. elata, a plant resource for homology of medicine and food,	1605:1687	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	10	6	theme	higher	1317:1322	arg1	antioxidant					1324:1334	higher antioxidant	1317:1334	higher antioxidant	1317:1334	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	3	7	theme	elata	494:498	arg1	Bl					500:501	G. elata Bl	491:501	G. elata Bl	491:501	f. elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl.
35936076	12	8	theme	G.	1749:1750	arg1	elata					1752:1756	G. elata	1749:1756	G. elata	1749:1756	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	9	9	theme	antioxidant	1128:1138	arg1	activity					1157:1164	antioxidant and hypoglycemic activity	1128:1164	antioxidant and hypoglycemic activity	1128:1164	However, GaE-B and GaE-Hyb were found with similar physical properties, chemical composition, and antioxidant and hypoglycemic activity.
35936076	1	10	theme	hypoglycemic	192:203	arg1	activity					205:212	hypoglycemic activity	192:212	hypoglycemic activity	192:212	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	12	11	theme	G.	1627:1628	arg1	resource					1645:1652	a plant resource	1637:1652	a plant resource for homology of medicine and food	1637:1686	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	12	11	theme	G.	1627:1628	arg1	elata					1630:1634	G. elata	1627:1634	G. elata	1627:1634	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	4	12	dep	Bl	535:536	arg1	S.					514:515	S.	514:515	S.	514:515	f. glauca S. chow and G. elata Bl.
35936076	4	12	dep	Bl	535:536	arg1	G.					526:527	G.	526:527	G.	526:527	f. glauca S. chow and G. elata Bl.
35936076	10	13	contain	had	1173:1175	arg2	yield					1188:1192	yield	1188:1192	yield	1188:1192	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	10	13	contain	had	1173:1175	arg2	weight					1230:1235	molecular weight	1220:1235	molecular weight	1220:1235	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	10	13	contain	had	1173:1175	arg1	GaE-R					1167:1171	GaE-R	1167:1171	GaE-R	1167:1171	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	10	13	contain	had	1173:1175	arg2	content					1207:1213	total sugar content	1195:1213	total sugar content	1195:1213	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	11	14	contain	contained	1470:1478	arg2	protein					1507:1513	binding protein	1499:1513	binding protein	1499:1513	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	14	contain	contained	1470:1478	arg2	xylose					1491:1496	the lowest xylose	1480:1496	the lowest xylose	1480:1496	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	14	contain	contained	1470:1478	arg2	polyphenols					1520:1530	polyphenols	1520:1530	polyphenols	1520:1530	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	14	contain	contained	1470:1478	arg1	it					1467:1468	it	1467:1468	it	1467:1468	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	10	15	theme	molecular	1220:1228	arg1	weight					1230:1235	molecular weight	1220:1235	molecular weight	1220:1235	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	1	16	theme	in	158:159	arg1	activity					178:185	in vitro biological activity	158:185	in vitro biological activity	158:185	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	10	17	theme	lowest	1181:1186	arg1	yield					1188:1192	yield	1188:1192	yield	1188:1192	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	7	18	dep	glucose	751:757	arg1	including					741:749	including	741:749	including	741:749	As revealed by the results, the GaE polysaccharides were found with the same monosaccharide composition, primarily including glucose, whereas the content of each variety was significantly different.
35936076	8	19	theme	molecular	937:945	arg1	weight					947:952	molecular weight	937:952	molecular weight	937:952	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	1	20	theme	biological	167:176	arg1	activity					178:185	in vitro biological activity	158:185	in vitro biological activity	158:185	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	0	21	from	Comparison	0:9	arg1	Bioactivities					14:26	Bioactivities	14:26	Bioactivities	14:26	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	0	21	from	Comparison	0:9	arg1	elata					100:104	Gastrodia elata	90:104	Gastrodia elata	90:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	0	21	from	Comparison	0:9	arg1	Varieties					77:85	Four Varieties	72:85	Four Varieties of Gastrodia elata	72:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	0	21	from	Comparison	0:9	arg1	Characteristics					32:46	Characteristics	32:46	Characteristics	32:46	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	11	22	theme	total	1416:1420	arg1	content					1428:1434	total sugar content	1416:1434	total sugar content	1416:1434	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	23	theme	highest	1401:1407	arg1	yield					1409:1413	the highest yield	1397:1413	the highest yield	1397:1413	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	0	24	theme	elata	100:104	arg1	Varieties					77:85	Four Varieties	72:85	Four Varieties of Gastrodia elata	72:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	0	24	theme	elata	100:104	arg1	elata					100:104	Gastrodia elata	90:104	Gastrodia elata	90:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	13	25	theme	activity	1867:1874	arg1	assessment					1838:1847	the assessment	1834:1847	the assessment of polysaccharide activity	1834:1874	To be specific, the assessment of polysaccharide activity alone suggested that GaE-R was the best.
35936076	5	26	theme	elata	542:546	arg1	polysaccharides					548:562	f. elata polysaccharides)	539:563	f. elata polysaccharides)	539:563	f. elata polysaccharides), and GaE-G (G. elata Bl.
35936076	3	27	dep	polysaccharides	447:461	arg1	hybridization					474:486	hybridization	474:486	hybridization	474:486	f. elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl.
35936076	3	27	dep	polysaccharides	447:461	arg1	GaE-Hyb					465:471	GaE-Hyb	465:471	GaE-Hyb	465:471	f. elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl.
35936076	8	28	dep	activity	927:934	arg1	the					885:887	the	885:887	the	885:887	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	7	29	theme	monosaccharide	703:716	arg1	composition					718:728	the same monosaccharide composition	694:728	the same monosaccharide composition	694:728	As revealed by the results, the GaE polysaccharides were found with the same monosaccharide composition, primarily including glucose, whereas the content of each variety was significantly different.
35936076	12	30	theme	biological	1726:1735	arg1	activity					1737:1744	the biological activity	1722:1744	the biological activity of G. elata	1722:1756	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	8	31	theme	chemical	966:973	arg1	composition					975:985	chemical composition	966:985	chemical composition	966:985	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	6	32	theme	viridis	593:599	arg1	polysaccharides					608:622	f. viridis Makino polysaccharides	590:622	f. viridis Makino polysaccharides	590:622	f. viridis Makino polysaccharides).
35936076	7	33	theme	same	698:701	arg1	composition					718:728	the same monosaccharide composition	694:728	the same monosaccharide composition	694:728	As revealed by the results, the GaE polysaccharides were found with the same monosaccharide composition, primarily including glucose, whereas the content of each variety was significantly different.
35936076	8	34	theme	different	838:846	arg1	degrees					848:854	different degrees	838:854	different degrees of differences	838:869	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	11	35	theme	weakest	1548:1554	arg1	activity					1585:1592	the weakest antioxidant and hypoglycemic activity	1544:1592	the weakest antioxidant and hypoglycemic activity	1544:1592	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	0	36	theme	Polysaccharides	51:65	arg1	Comparison					0:9	Comparison	0:9	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata	0:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	1	37	theme	extracted	317:325	arg1	polysaccharides					331:345	the four extracted GaE polysaccharides	308:345	the four extracted GaE polysaccharides	308:345	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	1	37	theme	extracted	317:325	arg1	GaE-B					362:366	GaE-B	362:366	GaE-B	362:366	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	12	38	theme	food	1683:1686	arg1	homology					1658:1665	homology	1658:1665	homology of medicine and food	1658:1686	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	13	39	theme	polysaccharide	1852:1865	arg1	activity					1867:1874	polysaccharide activity	1852:1874	polysaccharide activity	1852:1874	To be specific, the assessment of polysaccharide activity alone suggested that GaE-R was the best.
35936076	3	40	theme	elata	441:445	arg1	polysaccharides					447:461	elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl	441:501	polysaccharides	447:461	f. elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl.
35936076	7	41	theme	GaE	658:660	arg1	polysaccharides					662:676	the GaE polysaccharides	654:676	the GaE polysaccharides	654:676	As revealed by the results, the GaE polysaccharides were found with the same monosaccharide composition, primarily including glucose, whereas the content of each variety was significantly different.
35936076	12	42	theme	plant	1639:1643	arg1	resource					1645:1652	a plant resource	1637:1652	a plant resource for homology of medicine and food	1637:1686	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	12	42	theme	plant	1639:1643	arg1	elata					1630:1634	G. elata	1627:1634	G. elata	1627:1634	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	11	43	theme	binding	1499:1505	arg1	xylose					1491:1496	the lowest xylose	1480:1496	the lowest xylose	1480:1496	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	43	theme	binding	1499:1505	arg1	protein					1507:1513	binding protein	1499:1513	binding protein	1499:1513	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	3	44	dep	f.	438:439	arg1	polysaccharides					447:461	elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl	441:501	polysaccharides	447:461	f. elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl.
35936076	9	45	theme	physical	1081:1088	arg1	properties					1090:1099	similar physical properties	1073:1099	similar physical properties	1073:1099	However, GaE-B and GaE-Hyb were found with similar physical properties, chemical composition, and antioxidant and hypoglycemic activity.
35936076	1	46	theme	G.	266:267	arg1	elata					269:273	G. elata	266:273	G. elata	266:273	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	8	47	theme	in	889:890	arg1	activity					927:934	in vitro antioxidant and hypoglycemic activity	889:934	in vitro antioxidant and hypoglycemic activity	889:934	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	5	48	theme	elata	580:584	arg1	Bl					586:587	elata Bl	580:587	elata Bl	580:587	f. elata polysaccharides), and GaE-G (G. elata Bl.
35936076	3	49	theme	Bl	500:501	arg1	polysaccharides					447:461	elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl	441:501	polysaccharides	447:461	f. elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl.
35936076	7	50	theme	variety	788:794	arg1	content					772:778	the content	768:778	the content of each variety	768:794	As revealed by the results, the GaE polysaccharides were found with the same monosaccharide composition, primarily including glucose, whereas the content of each variety was significantly different.
35936076	7	50	theme	variety	788:794	arg1	different					814:822	different	814:822	different	814:822	As revealed by the results, the GaE polysaccharides were found with the same monosaccharide composition, primarily including glucose, whereas the content of each variety was significantly different.
35936076	4	51	theme	elata	529:533	arg1	Bl					535:536	elata Bl	529:536	elata Bl	529:536	f. glauca S. chow and G. elata Bl.
35936076	11	52	located	found	1386:1390	arg2	GaE-G					1376:1380	GaE-G	1376:1380	GaE-G	1376:1380	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	52	located	found	1386:1390	arg1	contrast					1366:1373	contrast	1366:1373	contrast	1366:1373	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	53	theme	sugar	1422:1426	arg1	content					1428:1434	total sugar content	1416:1434	total sugar content	1416:1434	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	3	54	theme	G.	491:492	arg1	Bl					500:501	G. elata Bl	491:501	G. elata Bl	491:501	f. elata polysaccharides), GaE-Hyb (hybridization of G. elata Bl.
35936076	12	55	theme	decocting	1795:1803	arg1	process					1784:1790	the process	1780:1790	the process of decocting and stewing	1780:1815	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	11	56	theme	molecular	1441:1449	arg1	weight					1451:1456	molecular weight	1441:1456	molecular weight	1441:1456	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	12	57	theme	stewing	1809:1815	arg1	process					1784:1790	the process	1780:1790	the process of decocting and stewing	1780:1815	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	0	58	from	Varieties	77:85	arg1	Comparison					0:9	Comparison	0:9	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata	0:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	0	58	from	Varieties	77:85	arg1	Polysaccharides					51:65	Polysaccharides	51:65	Polysaccharides From Four Varieties of Gastrodia elata	51:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	1	59	from	varieties	253:261	arg1	polysaccharides					227:241	polysaccharides	227:241	polysaccharides from four varieties of G. elata	227:273	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	1	60	theme	physicochemical	130:144	arg1	properties					146:155	physicochemical properties	130:155	physicochemical properties	130:155	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	0	61	theme	Gastrodia	90:98	arg1	elata					100:104	Gastrodia elata	90:104	Gastrodia elata	90:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	2	62	dep	Bl	434:435	arg1	GaE-R					418:422	GaE-R	418:422	GaE-R	418:422	f. glauca S. chow polysaccharides), GaE-R (G. elata Bl.
35936076	2	62	dep	Bl	434:435	arg1	S.					392:393	S.	392:393	S.	392:393	f. glauca S. chow polysaccharides), GaE-R (G. elata Bl.
35936076	10	63	theme	hypoglycemic	1340:1351	arg1	activity					1353:1360	hypoglycemic activity	1340:1360	hypoglycemic activity	1340:1360	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	9	64	theme	hypoglycemic	1144:1155	arg1	activity					1157:1164	antioxidant and hypoglycemic activity	1128:1164	antioxidant and hypoglycemic activity	1128:1164	However, GaE-B and GaE-Hyb were found with similar physical properties, chemical composition, and antioxidant and hypoglycemic activity.
35936076	5	65	theme	f.	539:540	arg1	polysaccharides					548:562	f. elata polysaccharides)	539:563	f. elata polysaccharides)	539:563	f. elata polysaccharides), and GaE-G (G. elata Bl.
35936076	4	66	dep	S.	514:515	arg1	chow					517:520	chow	517:520	chow	517:520	f. glauca S. chow and G. elata Bl.
35936076	0	67	dep	Blume	106:110	arg1	Comparison					0:9	Comparison	0:9	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata	0:104	Comparison on Bioactivities and Characteristics of Polysaccharides From Four Varieties of Gastrodia elata Blume.
35936076	10	68	theme	total	1195:1199	arg1	content					1207:1213	total sugar content	1195:1213	total sugar content	1195:1213	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	1	69	theme	elata	372:376	arg1	Bl					378:379	elata Bl	372:379	G. elata Bl	369:379	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	12	70	theme	elata	1752:1756	arg1	activity					1737:1744	the biological activity	1722:1744	the biological activity of G. elata	1722:1756	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	5	71	dep	Bl	586:587	arg1	G.					577:578	G.	577:578	G.	577:578	f. elata polysaccharides), and GaE-G (G. elata Bl.
35936076	1	72	dep	termed	352:357	arg1	G.					369:370	G.	369:370	G.	369:370	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	6	73	theme	Makino	601:606	arg1	polysaccharides					608:622	f. viridis Makino polysaccharides	590:622	f. viridis Makino polysaccharides	590:622	f. viridis Makino polysaccharides).
35936076	11	74	theme	antioxidant	1556:1566	arg1	activity					1585:1592	the weakest antioxidant and hypoglycemic activity	1544:1592	the weakest antioxidant and hypoglycemic activity	1544:1592	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	1	75	dep	in	158:159	arg1	vitro					161:165	vitro	161:165	vitro	161:165	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	8	76	dep	in	889:890	arg1	vitro					892:896	vitro	892:896	vitro	892:896	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	8	76	dep	in	889:890	arg1	hypoglycemic					914:925	hypoglycemic	914:925	hypoglycemic	914:925	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	8	76	dep	in	889:890	arg1	antioxidant					898:908	antioxidant	898:908	antioxidant	898:908	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	10	77	theme	sugar	1201:1205	arg1	content					1207:1213	total sugar content	1195:1213	total sugar content	1195:1213	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	8	78	theme	abovementioned	1004:1017	arg1	varieties					1019:1027	the abovementioned varieties	1000:1027	the abovementioned varieties	1000:1027	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	1	79	dep	G.	369:370	arg1	Bl					378:379	elata Bl	372:379	G. elata Bl	369:379	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	2	80	dep	S.	392:393	arg1	chow					395:398	chow	395:398	chow polysaccharides	395:414	f. glauca S. chow polysaccharides), GaE-R (G. elata Bl.
35936076	2	80	dep	S.	392:393	arg1	G.					425:426	G.	425:426	G.	425:426	f. glauca S. chow polysaccharides), GaE-R (G. elata Bl.
35936076	2	81	theme	elata	428:432	arg1	Bl					434:435	elata Bl	428:435	elata Bl	428:435	f. glauca S. chow polysaccharides), GaE-R (G. elata Bl.
35936076	8	82	located	found	876:880	arg1	weight					947:952	molecular weight	937:952	molecular weight	937:952	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	8	82	located	found	876:880	arg1	addition					828:835	addition	828:835	addition	828:835	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	8	82	located	found	876:880	arg1	yield					955:959	yield	955:959	yield	955:959	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	8	82	located	found	876:880	arg2	degrees					848:854	different degrees	838:854	different degrees of differences	838:869	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	8	82	located	found	876:880	arg1	activity					927:934	in vitro antioxidant and hypoglycemic activity	889:934	in vitro antioxidant and hypoglycemic activity	889:934	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	8	82	located	found	876:880	arg1	composition					975:985	chemical composition	966:985	chemical composition	966:985	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	1	83	theme	GaE	327:329	arg1	polysaccharides					331:345	the four extracted GaE polysaccharides	308:345	the four extracted GaE polysaccharides	308:345	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	1	83	theme	GaE	327:329	arg1	GaE-B					362:366	GaE-B	362:366	GaE-B	362:366	The composition, physicochemical properties, in vitro biological activity, and hypoglycemic activity exhibited by polysaccharides from four varieties of G. elata were investigated in this study; the four extracted GaE polysaccharides were termed as GaE-B (G. elata Bl.
35936076	12	84	theme	medicine	1670:1677	arg1	homology					1658:1665	homology	1658:1665	homology of medicine and food	1658:1686	In brief, the polysaccharide of G. elata, a plant resource for homology of medicine and food, could more significantly enhance the biological activity of G. elata as it was released in the process of decocting and stewing.
35936076	9	85	theme	similar	1073:1079	arg1	properties					1090:1099	similar physical properties	1073:1099	similar physical properties	1073:1099	However, GaE-B and GaE-Hyb were found with similar physical properties, chemical composition, and antioxidant and hypoglycemic activity.
35936076	11	86	theme	hypoglycemic	1572:1583	arg1	activity					1585:1592	the weakest antioxidant and hypoglycemic activity	1544:1592	the weakest antioxidant and hypoglycemic activity	1544:1592	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	10	87	theme	binding	1273:1279	arg1	protein					1281:1287	binding protein	1273:1287	binding protein	1273:1287	GaE-R had the lowest yield, total sugar content, and molecular weight, whereas it involved higher xylose, binding protein, and polyphenols as well as higher antioxidant and hypoglycemic activity.
35936076	8	88	theme	differences	859:869	arg1	degrees					848:854	different degrees	838:854	different degrees of differences	838:869	In addition, different degrees of differences were found in the in vitro antioxidant and hypoglycemic activity, molecular weight, yield, and chemical composition exhibited by the abovementioned varieties.
35936076	11	89	theme	lowest	1484:1489	arg1	xylose					1491:1496	the lowest xylose	1480:1496	the lowest xylose	1480:1496	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35936076	11	89	theme	lowest	1484:1489	arg1	protein					1507:1513	binding protein	1499:1513	binding protein	1499:1513	In contrast, GaE-G was found with the highest yield, total sugar content, and molecular weight, whereas it contained the lowest xylose, binding protein, and polyphenols, as well as the weakest antioxidant and hypoglycemic activity.
35961465	5	0	theme	percent	1070:1076	arg1	GP					1078:1079	Ten percent GP	1066:1079	Ten percent GP	1066:1079	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	5	1	from	decrease	1143:1150	arg1	composition					1199:1209	microbial composition	1189:1209	microbial composition	1189:1209	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	4	2	theme	%	971:971	arg1	GP					973:974	10% GP	969:974	10% GP	969:974	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	0	3	from	colitis	159:165	arg1	mice					189:192	antibiotic-treated mice	170:192	antibiotic-treated mice	170:192	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	4	4	theme	AOM-DSS-induced	1003:1017	arg1	colitis					1019:1025	AOM-DSS-induced colitis	1003:1025	AOM-DSS-induced colitis	1003:1025	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	6	5	theme	control	1405:1411	arg1	diet					1413:1416	the control diet	1401:1416	the control diet	1401:1416	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	4	6	theme	%	986:986	arg1	GP					988:989	3% GP	985:989	3% GP	985:989	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	3	7	theme	sulfate	715:721	arg1	colitis					744:750	dextran sulfate sodium (DSS)-induced colitis	707:750	dextran sulfate sodium (DSS)-induced colitis	707:750	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	6	8	theme	diseased	1383:1390	arg1	mice					1392:1395	diseased mice	1383:1395	diseased mice fed the control diet	1383:1416	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	2	9	theme	cancer	554:559	arg1	attenuation					502:512	the attenuation	498:512	the attenuation of colitis and colitis-associated colon cancer (CAC)	498:565	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	2	10	with	interaction	432:442	arg1	microbiota					472:481	the gut microbiota	464:481	the gut microbiota	464:481	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	7	11	theme	bacteria	1494:1501	arg1	abundance					1462:1470	the relative abundance	1449:1470	the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family	1449:1531	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	9	12	theme	disease-associated	1816:1833	arg1	dysbiosis					1835:1843	disease-associated dysbiosis	1816:1843	disease-associated dysbiosis	1816:1843	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	5	13	theme	evenness	1165:1172	arg1	decrease					1143:1150	CAC-induced decrease	1131:1150	CAC-induced decrease of bacterial evenness	1131:1172	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	5	13	theme	evenness	1165:1172	arg1	changes					1178:1184	changes	1178:1184	changes in microbial composition	1178:1209	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	7	14	theme	relative	1453:1460	arg1	abundance					1462:1470	the relative abundance	1449:1470	the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family	1449:1531	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	6	15	theme	mice	1283:1286	arg1	profiles					1263:1270	the estimated gut bacterial functional profiles	1224:1270	the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP	1224:1315	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	6	15	theme	mice	1283:1286	arg1	similar					1322:1328	similar	1322:1328	similar	1322:1328	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	1	16	theme	microbiota	314:323	arg1	modulation					325:334	gut microbiota modulation	310:334	gut microbiota modulation by polyphenols	310:349	Polyphenols are known to interact with gut microbes that play key roles in maintaining gut health, but the role of gut microbiota modulation by polyphenols in mitigating colonic diseases is not fully established.
35961465	6	17	theme	%	1312:1312	arg1	GP					1314:1315	10% GP	1310:1315	10% GP	1310:1315	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	8	18	theme	antibiotic-treated	1669:1686	arg1	mice					1710:1713	the antibiotic-treated, gut microbe-depleted mice	1665:1713	the antibiotic-treated, gut microbe-depleted mice	1665:1713	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	9	19	theme	causative	1903:1911	arg1	role					1913:1916	a causative role	1901:1916	a causative role	1901:1916	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	5	20	theme	CAC-induced	1131:1141	arg1	decrease					1143:1150	CAC-induced decrease	1131:1150	CAC-induced decrease of bacterial evenness	1131:1172	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	7	21	theme	%	1434:1434	arg1	GP					1436:1437	10% GP	1432:1437	10% GP	1432:1437	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	2	22	theme	colitis-associated	529:546	arg1	CAC					562:564	CAC	562:564	CAC	562:564	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	2	22	theme	colitis-associated	529:546	arg1	cancer					554:559	colitis-associated colon cancer	529:559	colitis-associated colon cancer (CAC)	529:565	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	3	23	from	colitis	744:750	arg1	study					790:794	study 1	790:796	study 1	790:796	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	23	from	colitis	744:750	arg1	mice					784:787	mice	784:787	mice (study 1)	784:797	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	24	theme	gut	766:768	arg1	microbiota					770:779	the gut microbiota	762:779	the gut microbiota	762:779	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	0	25	theme	disease-associated	99:116	arg1	dysbiosis					118:126	disease-associated dysbiosis	99:126	disease-associated dysbiosis	99:126	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	7	26	theme	butyrate	1574:1581	arg1	concentrations					1550:1563	the concentrations	1546:1563	the concentrations of fecal butyrate	1546:1581	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	2	27	theme	colitis	517:523	arg1	attenuation					502:512	the attenuation	498:512	the attenuation of colitis and colitis-associated colon cancer (CAC)	498:565	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	6	28	theme	gut	1238:1240	arg1	profiles					1263:1270	the estimated gut bacterial functional profiles	1224:1270	the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP	1224:1315	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	6	28	theme	gut	1238:1240	arg1	similar					1322:1328	similar	1322:1328	similar	1322:1328	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	1	29	theme	colonic	365:371	arg1	diseases					373:380	colonic diseases	365:380	colonic diseases	365:380	Polyphenols are known to interact with gut microbes that play key roles in maintaining gut health, but the role of gut microbiota modulation by polyphenols in mitigating colonic diseases is not fully established.
35961465	3	30	theme	anti-colitis	821:832	arg1	effects					834:840	anti-colitis effects	821:840	anti-colitis effects of GP	821:846	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	2	31	theme	polyphenols	447:457	arg1	interaction					432:442	the interaction	428:442	the interaction of polyphenols with the gut microbiota	428:481	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	9	32	theme	gut	1939:1941	arg1	health					1943:1948	gut health	1939:1948	gut health	1939:1948	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	1	33	theme	gut	282:284	arg1	health					286:291	gut health	282:291	gut health	282:291	Polyphenols are known to interact with gut microbes that play key roles in maintaining gut health, but the role of gut microbiota modulation by polyphenols in mitigating colonic diseases is not fully established.
35961465	9	34	with	interaction	1856:1866	arg1	microbiota					1881:1890	the gut microbiota	1873:1890	the gut microbiota	1873:1890	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	5	35	theme	microbial	1189:1197	arg1	composition					1199:1209	microbial composition	1189:1209	microbial composition	1189:1209	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	0	36	theme	polyphenol-rich	19:33	arg1	grapes					35:40	polyphenol-rich grapes	19:40	polyphenol-rich grapes	19:40	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	4	37	theme	control	914:920	arg1	diet					922:925	the control diet	910:925	the control diet that has matched non-polyphenol contents	910:966	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	0	38	theme	colon	81:85	arg1	cancer					87:92	colitis-associated colon cancer	62:92	colitis-associated colon cancer	62:92	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	3	39	theme	polyphenol-rich	647:661	arg1	powder					669:674	polyphenol-rich grape powder	647:674	polyphenol-rich grape powder (GP)	647:679	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	39	theme	polyphenol-rich	647:661	arg1	GP					677:678	GP	677:678	GP	677:678	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	40	from	CAC	753:755	arg1	study					790:794	study 1	790:796	study 1	790:796	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	40	from	CAC	753:755	arg1	mice					784:787	mice	784:787	mice (study 1)	784:797	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	41	theme	supplementation	628:642	arg1	effects					609:615	the effects	605:615	the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1)	605:797	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	6	42	theme	diseased	1292:1299	arg1	mice					1301:1304	diseased mice	1292:1304	diseased mice	1292:1304	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	8	43	dep	antibiotic-treated	1669:1686	arg1	microbe-depleted					1693:1708	microbe-depleted	1693:1708	microbe-depleted	1693:1708	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	8	44	from	colitis	1627:1633	arg1	mice					1651:1654	conventional mice	1638:1654	conventional mice	1638:1654	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	8	44	from	colitis	1627:1633	arg1	mice					1710:1713	the antibiotic-treated, gut microbe-depleted mice	1665:1713	the antibiotic-treated, gut microbe-depleted mice	1665:1713	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	5	45	theme	gut	1091:1093	arg1	evenness					1105:1112	gut bacterial evenness	1091:1112	gut bacterial evenness	1091:1112	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	4	46	theme	3	985:985	arg1	%					986:986	%	986:986	%	986:986	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	8	47	theme	%	1600:1600	arg1	GP					1602:1603	10% GP	1598:1603	10% GP	1598:1603	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	3	48	theme	powder	669:674	arg1	supplementation					628:642	dietary supplementation	620:642	dietary supplementation of polyphenol-rich grape powder (GP)	620:679	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	6	49	theme	mice	1301:1304	arg1	profiles					1263:1270	the estimated gut bacterial functional profiles	1224:1270	the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP	1224:1315	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	6	49	theme	mice	1301:1304	arg1	similar					1322:1328	similar	1322:1328	similar	1322:1328	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	4	50	theme	10	969:970	arg1	%					971:971	%	971:971	%	971:971	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	1	51	theme	key	257:259	arg1	roles					261:265	key roles	257:265	key roles	257:265	Polyphenols are known to interact with gut microbes that play key roles in maintaining gut health, but the role of gut microbiota modulation by polyphenols in mitigating colonic diseases is not fully established.
35961465	9	52	theme	colonic	1787:1793	arg1	diseases					1795:1802	colonic diseases	1787:1802	colonic diseases	1787:1802	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	3	53	theme	dextran	707:713	arg1	colitis					744:750	dextran sulfate sodium (DSS)-induced colitis	707:750	dextran sulfate sodium (DSS)-induced colitis	707:750	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	4	54	theme	tumor	1031:1035	arg1	multiplicity					1037:1048	tumor multiplicity	1031:1048	tumor multiplicity	1031:1048	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	8	55	theme	10	1598:1599	arg1	%					1600:1600	%	1600:1600	%	1600:1600	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	7	56	theme	Lachnospiraceae	1510:1524	arg1	family					1526:1531	the Lachnospiraceae family	1506:1531	the Lachnospiraceae family	1506:1531	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	4	57	contain	has	932:934	arg1	diet					922:925	the control diet	910:925	the control diet that has matched non-polyphenol contents	910:966	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	4	57	contain	has	932:934	arg2	contents					959:966	matched non-polyphenol contents	936:966	matched non-polyphenol contents	936:966	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	5	58	from	changes	1178:1184	arg1	composition					1199:1209	microbial composition	1189:1209	microbial composition	1189:1209	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	3	59	theme	DSS	731:733	arg1	colitis					744:750	dextran sulfate sodium (DSS)-induced colitis	707:750	dextran sulfate sodium (DSS)-induced colitis	707:750	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	6	60	theme	estimated	1228:1236	arg1	profiles					1263:1270	the estimated gut bacterial functional profiles	1224:1270	the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP	1224:1315	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	6	60	theme	estimated	1228:1236	arg1	similar					1322:1328	similar	1322:1328	similar	1322:1328	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	3	61	theme	sodium	723:728	arg1	colitis					744:750	dextran sulfate sodium (DSS)-induced colitis	707:750	dextran sulfate sodium (DSS)-induced colitis	707:750	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	8	62	theme	DSS-induced	1615:1625	arg1	colitis					1627:1633	DSS-induced colitis	1615:1633	DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice	1615:1713	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	7	63	theme	butyrate-producing	1475:1492	arg1	bacteria					1494:1501	butyrate-producing bacteria	1475:1501	butyrate-producing bacteria	1475:1501	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	2	64	theme	colon	548:552	arg1	CAC					562:564	CAC	562:564	CAC	562:564	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	2	64	theme	colon	548:552	arg1	cancer					554:559	colitis-associated colon cancer	529:559	colitis-associated colon cancer (CAC)	529:565	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	0	65	from	cancer	87:92	arg1	mice					131:134	mice	131:134	mice	131:134	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	3	66	from	azoxymethane	684:695	arg1	study					790:794	study 1	790:796	study 1	790:796	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	66	from	azoxymethane	684:695	arg1	mice					784:787	mice	784:787	mice (study 1)	784:797	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	9	67	theme	gut	1877:1879	arg1	microbiota					1881:1890	the gut microbiota	1873:1890	the gut microbiota	1873:1890	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	5	68	theme	bacterial	1155:1163	arg1	evenness					1165:1172	bacterial evenness	1155:1172	bacterial evenness	1155:1172	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	3	69	theme	-induced	735:742	arg1	colitis					744:750	dextran sulfate sodium (DSS)-induced colitis	707:750	dextran sulfate sodium (DSS)-induced colitis	707:750	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	0	70	theme	antibiotic-treated	170:187	arg1	mice					189:192	antibiotic-treated mice	170:192	antibiotic-treated mice	170:192	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	0	71	from	dysbiosis	118:126	arg1	mice					131:134	mice	131:134	mice	131:134	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	1	72	theme	gut	234:236	arg1	microbes					238:245	gut microbes	234:245	gut microbes that play key roles in maintaining gut health	234:291	Polyphenols are known to interact with gut microbes that play key roles in maintaining gut health, but the role of gut microbiota modulation by polyphenols in mitigating colonic diseases is not fully established.
35961465	6	73	theme	functional	1252:1261	arg1	profiles					1263:1270	the estimated gut bacterial functional profiles	1224:1270	the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP	1224:1315	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	6	73	theme	functional	1252:1261	arg1	similar					1322:1328	similar	1322:1328	similar	1322:1328	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	6	74	theme	healthy	1275:1281	arg1	mice					1283:1286	healthy mice	1275:1286	healthy mice	1275:1286	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	1	75	theme	modulation	325:334	arg1	role					302:305	the role	298:305	the role of gut microbiota modulation by polyphenols in mitigating colonic diseases	298:380	Polyphenols are known to interact with gut microbes that play key roles in maintaining gut health, but the role of gut microbiota modulation by polyphenols in mitigating colonic diseases is not fully established.
35961465	7	76	from	abundance	1462:1470	arg1	family					1526:1531	the Lachnospiraceae family	1506:1531	the Lachnospiraceae family	1506:1531	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	7	77	theme	10	1432:1433	arg1	%					1434:1434	%	1434:1434	%	1434:1434	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	6	78	theme	bacterial	1242:1250	arg1	profiles					1263:1270	the estimated gut bacterial functional profiles	1224:1270	the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP	1224:1315	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	6	78	theme	bacterial	1242:1250	arg1	similar					1322:1328	similar	1322:1328	similar	1322:1328	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	3	79	theme	GP	845:846	arg1	effects					834:840	anti-colitis effects	821:840	anti-colitis effects of GP	821:846	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	0	80	from	colitis	53:59	arg1	mice					131:134	mice	131:134	mice	131:134	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	3	81	from	effects	609:615	arg1	azoxymethane					684:695	azoxymethane	684:695	azoxymethane (AOM)	684:701	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	81	from	effects	609:615	arg1	colitis					744:750	dextran sulfate sodium (DSS)-induced colitis	707:750	dextran sulfate sodium (DSS)-induced colitis	707:750	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	81	from	effects	609:615	arg1	AOM					698:700	AOM	698:700	AOM	698:700	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	7	82	theme	fecal	1568:1572	arg1	butyrate					1574:1581	fecal butyrate	1568:1581	fecal butyrate	1568:1581	Furthermore, 10% GP increased the relative abundance of butyrate-producing bacteria in the Lachnospiraceae family and enhanced the concentrations of fecal butyrate.
35961465	9	83	theme	health	1943:1948	arg1	protection					1925:1934	the protection	1921:1934	the protection of gut health	1921:1948	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	0	84	theme	grapes	35:40	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of polyphenol-rich grapes	0:40	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	3	85	theme	antibiotic-treated	863:880	arg1	study					888:892	study 2	888:894	study 2	888:894	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	85	theme	antibiotic-treated	863:880	arg1	mice					882:885	regular and antibiotic-treated mice	851:885	regular and antibiotic-treated mice (study 2)	851:895	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	4	86	theme	non-polyphenol	944:957	arg1	contents					959:966	matched non-polyphenol contents	936:966	matched non-polyphenol contents	936:966	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35961465	3	87	from	microbiota	770:779	arg1	study					790:794	study 1	790:796	study 1	790:796	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	87	from	microbiota	770:779	arg1	mice					784:787	mice	784:787	mice (study 1)	784:797	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	6	88	theme	10	1310:1311	arg1	%					1312:1312	%	1312:1312	%	1312:1312	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	3	89	theme	regular	851:857	arg1	study					888:892	study 2	888:894	study 2	888:894	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	89	theme	regular	851:857	arg1	mice					882:885	regular and antibiotic-treated mice	851:885	regular and antibiotic-treated mice (study 2)	851:895	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	2	90	theme	gut	468:470	arg1	microbiota					472:481	the gut microbiota	464:481	the gut microbiota	464:481	We hypothesize that the interaction of polyphenols with the gut microbiota contributes to the attenuation of colitis and colitis-associated colon cancer (CAC).
35961465	3	91	theme	dietary	620:626	arg1	supplementation					628:642	dietary supplementation	620:642	dietary supplementation of polyphenol-rich grape powder (GP)	620:679	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	0	92	theme	colitis-associated	62:79	arg1	cancer					87:92	colitis-associated colon cancer	62:92	colitis-associated colon cancer	62:92	Supplementation of polyphenol-rich grapes attenuates colitis, colitis-associated colon cancer, and disease-associated dysbiosis in mice, but fails to mitigate colitis in antibiotic-treated mice.
35961465	6	93	from	those	1374:1378	arg1	different					1359:1367	different	1359:1367	different	1359:1367	Remarkably, the estimated gut bacterial functional profiles of healthy mice and diseased mice fed 10% GP were similar, and both were significantly different from those of diseased mice fed the control diet.
35961465	1	94	theme	gut	310:312	arg1	modulation					325:334	gut microbiota modulation	310:334	gut microbiota modulation by polyphenols	310:349	Polyphenols are known to interact with gut microbes that play key roles in maintaining gut health, but the role of gut microbiota modulation by polyphenols in mitigating colonic diseases is not fully established.
35961465	3	95	theme	grape	663:667	arg1	powder					669:674	polyphenol-rich grape powder	647:674	polyphenol-rich grape powder (GP)	647:679	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	3	95	theme	grape	663:667	arg1	GP					677:678	GP	677:678	GP	677:678	To test this hypothesis, we examined the effects of dietary supplementation of polyphenol-rich grape powder (GP) on azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced colitis, CAC, and the gut microbiota in mice (study 1), and further compared anti-colitis effects of GP in regular and antibiotic-treated mice (study 2).
35961465	8	96	theme	conventional	1638:1649	arg1	mice					1651:1654	conventional mice	1638:1654	conventional mice	1638:1654	Additionally, 10% GP mitigated DSS-induced colitis in conventional mice, but not the antibiotic-treated, gut microbe-depleted mice.
35961465	5	97	theme	bacterial	1095:1103	arg1	evenness					1105:1112	gut bacterial evenness	1091:1112	gut bacterial evenness	1091:1112	Ten percent GP increased gut bacterial evenness and counteracted CAC-induced decrease of bacterial evenness and changes in microbial composition.
35961465	9	98	theme	grape	1759:1763	arg1	polyphenols					1765:1775	grape polyphenols	1759:1775	grape polyphenols	1759:1775	Collectively, our studies demonstrate that grape polyphenols alleviate colonic diseases and prevent disease-associated dysbiosis, and their interaction with the gut microbiota may play a causative role in the protection of gut health.
35961465	4	99	theme	matched	936:942	arg1	contents					959:966	matched non-polyphenol contents	936:966	matched non-polyphenol contents	936:966	Compared to the control diet that has matched non-polyphenol contents, 10% GP, but not 3% GP, attenuated AOM-DSS-induced colitis and tumor multiplicity by 29% (P<.05).
35132375	1	0	theme	bowel	151:155	arg1	colitis					124:130	Ulcerative colitis	113:130	Ulcerative colitis	113:130	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	1	0	theme	bowel	151:155	arg1	disease					157:163	an inflammatory bowel disease	135:163	an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis	135:299	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	4	1	from	changes	691:697	arg1	composition					728:738	the intestinal microbiome composition	702:738	the intestinal microbiome composition	702:738	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	5	2	theme	study	901:905	arg1	product					907:913	the study product	897:913	the study product	897:913	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	0	3	from	Potential	12:20	arg1	Milk					77:80	Mare's Milk	70:80	Mare's Milk	70:80	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	0	3	from	Potential	12:20	arg1	Lactobacillus					42:54	Lactobacillus	42:54	Lactobacillus	42:54	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	0	3	from	Potential	12:20	arg1	Treatment					89:97	the Treatment	85:97	the Treatment of Dysbiosis	85:110	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	5	4	from	effect	887:892	arg1	dysbiosis					949:957	dextran sulfate sodium-induced dysbiosis	918:957	dextran sulfate sodium-induced dysbiosis	918:957	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	6	5	theme	product	1111:1117	arg1	influence					1085:1093	the influence	1081:1093	the influence of a biological product	1081:1117	The level of proinflammatory cytokines also decreased under the influence of a biological product.
35132375	3	6	from	milk	525:528	arg1	effects					455:461	the potential therapeutic effects	429:461	the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines	429:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	3	6	from	milk	525:528	arg1	metabolites					473:483	active metabolites	466:483	active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines	466:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	3	7	from	Lactobacillus	490:502	arg1	effects					455:461	the potential therapeutic effects	429:461	the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines	429:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	3	7	from	Lactobacillus	490:502	arg1	metabolites					473:483	active metabolites	466:483	active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines	466:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	1	8	theme	gut	245:247	arg1	microbiota					249:258	gut microbiota	245:258	gut microbiota	245:258	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	0	9	theme	Dysbiosis	102:110	arg1	Treatment					89:97	the Treatment	85:97	the Treatment of Dysbiosis	85:110	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	3	10	theme	active	466:471	arg1	metabolites					473:483	active metabolites	466:483	active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines	466:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	6	11	theme	cytokines	1050:1058	arg1	level					1025:1029	The level	1021:1029	The level of proinflammatory cytokines	1021:1058	The level of proinflammatory cytokines also decreased under the influence of a biological product.
35132375	3	12	theme	current	405:411	arg1	study					413:417	The current study	401:417	The current study	401:417	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	0	13	from	Lactobacillus	42:54	arg1	Metabolites					25:35	Metabolites	25:35	Metabolites from Lactobacillus rhamnosus and Mare's Milk	25:80	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	0	13	from	Lactobacillus	42:54	arg1	Potential					12:20	Therapeutic Potential	0:20	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.	0:111	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	4	14	theme	positive	804:811	arg1	group					821:825	the positive control group	800:825	the positive control group that was treated with 5-aminosalicylic acid (5-ASA)	800:877	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	3	15	from	effects	455:461	arg1	milk					525:528	mare's milk	518:528	mare's milk	518:528	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	3	15	from	effects	455:461	arg1	Lactobacillus					490:502	Lactobacillus	490:502	Lactobacillus	490:502	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	4	16	theme	treated	774:780	arg1	group					782:786	the treated group	770:786	the treated group	770:786	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	4	17	theme	V1-V3	650:654	arg1	site					665:668	the V1-V3 16S rDNA site	646:668	the V1-V3 16S rDNA site	646:668	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	1	18	theme	pivotal	268:274	arg1	role					276:279	a pivotal role	266:279	a pivotal role	266:279	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	5	19	theme	dextran	918:924	arg1	sulfate					926:932	dextran sulfate	918:932	dextran sulfate sodium-induced dysbiosis	918:957	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	4	20	theme	site	665:668	arg1	analysis					634:641	The analysis	630:641	The analysis of the V1-V3 16S rDNA site	630:668	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	0	21	theme	Therapeutic	0:10	arg1	Potential					12:20	Therapeutic Potential	0:20	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.	0:111	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	4	22	theme	microbiome	717:726	arg1	composition					728:738	the intestinal microbiome composition	702:738	the intestinal microbiome composition	702:738	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	3	23	dep	Lactobacillus	490:502	arg1	rhamnosus					504:512	Lactobacillus rhamnosus	490:512	Lactobacillus rhamnosus	490:512	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	4	24	theme	rDNA	660:663	arg1	site					665:668	the V1-V3 16S rDNA site	646:668	the V1-V3 16S rDNA site	646:668	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	0	25	theme	Metabolites	25:35	arg1	Potential					12:20	Therapeutic Potential	0:20	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.	0:111	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	3	26	contain	have	536:539	arg1	milk					525:528	mare's milk	518:528	mare's milk	518:528	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	3	26	contain	have	536:539	arg2	values					563:568	potential therapeutic values	541:568	potential therapeutic values	541:568	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	3	26	contain	have	536:539	arg1	Lactobacillus					490:502	Lactobacillus	490:502	Lactobacillus	490:502	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	5	27	theme	sodium-induced	934:947	arg1	dysbiosis					949:957	dextran sulfate sodium-induced dysbiosis	918:957	dextran sulfate sodium-induced dysbiosis	918:957	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	5	28	theme	positive	996:1003	arg1	control					1005:1011	the positive control	992:1011	the positive control	992:1011	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	5	28	theme	positive	996:1003	arg1	5-ASA					1014:1018	5-ASA	1014:1018	5-ASA	1014:1018	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	2	29	theme	disease	392:398	arg1	remission					374:382	the remission	370:382	the remission of this disease	370:398	Agents modulating microbial dysbiosis caused by colitis can help in the remission of this disease.
35132375	5	30	theme	sulfate	926:932	arg1	dysbiosis					949:957	dextran sulfate sodium-induced dysbiosis	918:957	dextran sulfate sodium-induced dysbiosis	918:957	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	3	31	theme	potential	541:549	arg1	values					563:568	potential therapeutic values	541:568	potential therapeutic values	541:568	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	1	32	theme	mucous	195:200	arg1	membrane					202:209	the mucous membrane	191:209	the mucous membrane of the colon and rectum	191:233	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	3	33	theme	metabolites	473:483	arg1	effects					455:461	the potential therapeutic effects	429:461	the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines	429:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	0	34	from	Milk	77:80	arg1	Metabolites					25:35	Metabolites	25:35	Metabolites from Lactobacillus rhamnosus and Mare's Milk	25:80	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	0	34	from	Milk	77:80	arg1	Potential					12:20	Therapeutic Potential	0:20	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.	0:111	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	3	35	theme	intestinal	577:586	arg1	microbiota					588:597	the intestinal microbiota	573:597	the intestinal microbiota	573:597	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	6	36	theme	proinflammatory	1034:1048	arg1	cytokines					1050:1058	proinflammatory cytokines	1034:1058	proinflammatory cytokines	1034:1058	The level of proinflammatory cytokines also decreased under the influence of a biological product.
35132375	4	37	theme	16S	656:658	arg1	site					665:668	the V1-V3 16S rDNA site	646:668	the V1-V3 16S rDNA site	646:668	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	6	38	theme	biological	1100:1109	arg1	product					1111:1117	a biological product	1098:1117	a biological product	1098:1117	The level of proinflammatory cytokines also decreased under the influence of a biological product.
35132375	4	39	theme	control	813:819	arg1	group					821:825	the positive control group	800:825	the positive control group that was treated with 5-aminosalicylic acid (5-ASA)	800:877	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	1	40	dep	colon	218:222	arg1	the					214:216	the	214:216	the	214:216	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	0	41	dep	Lactobacillus	42:54	arg1	rhamnosus					56:64	Lactobacillus rhamnosus	42:64	Lactobacillus rhamnosus	42:64	Therapeutic Potential of Metabolites from Lactobacillus rhamnosus and Mare's Milk in the Treatment of Dysbiosis.
35132375	4	42	theme	5-aminosalicylic	849:864	arg1	5-ASA					872:876	5-ASA	872:876	5-ASA	872:876	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	4	42	theme	5-aminosalicylic	849:864	arg1	acid					866:869	5-aminosalicylic acid	849:869	5-aminosalicylic acid (5-ASA)	849:877	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	2	43	theme	microbial	320:328	arg1	dysbiosis					330:338	microbial dysbiosis	320:338	microbial dysbiosis caused by colitis	320:356	Agents modulating microbial dysbiosis caused by colitis can help in the remission of this disease.
35132375	1	44	theme	Ulcerative	113:122	arg1	colitis					124:130	Ulcerative colitis	113:130	Ulcerative colitis	113:130	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	1	44	theme	Ulcerative	113:122	arg1	disease					157:163	an inflammatory bowel disease	135:163	an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis	135:299	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	4	45	theme	intestinal	706:715	arg1	composition					728:738	the intestinal microbiome composition	702:738	the intestinal microbiome composition	702:738	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	5	46	theme	product	907:913	arg1	effect					887:892	So the effect	880:892	So the effect of the study product on dextran sulfate sodium-induced dysbiosis	880:957	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	5	46	theme	product	907:913	arg1	potent					980:985	potent	980:985	potent	980:985	So the effect of the study product on dextran sulfate sodium-induced dysbiosis was shown to be more potent than the positive control, 5-ASA.
35132375	1	47	from	ulcerations	176:186	arg1	membrane					202:209	the mucous membrane	191:209	the mucous membrane of the colon and rectum	191:233	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	3	48	dep	potential	541:549	arg1	therapeutic					551:561	therapeutic	551:561	therapeutic	551:561	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	1	49	theme	colon	218:222	arg1	membrane					202:209	the mucous membrane	191:209	the mucous membrane of the colon and rectum	191:233	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	3	50	dep	potential	433:441	arg1	therapeutic					443:453	therapeutic	443:453	therapeutic	443:453	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	3	51	theme	potential	433:441	arg1	effects					455:461	the potential therapeutic effects	429:461	the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines	429:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	4	52	theme	significant	679:689	arg1	changes					691:697	significant changes	679:697	significant changes in the intestinal microbiome composition before and after treatment	679:765	The analysis of the V1-V3 16S rDNA site revealed significant changes in the intestinal microbiome composition before and after treatment in the treated group compared to the positive control group that was treated with 5-aminosalicylic acid (5-ASA).
35132375	3	53	theme	proinflammatory	603:617	arg1	cytokines					619:627	proinflammatory cytokines	603:627	proinflammatory cytokines	603:627	The current study describes the potential therapeutic effects of active metabolites from Lactobacillus rhamnosus and mare's milk which have potential therapeutic values on the intestinal microbiota and proinflammatory cytokines.
35132375	1	54	theme	inflammatory	138:149	arg1	colitis					124:130	Ulcerative colitis	113:130	Ulcerative colitis	113:130	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	1	54	theme	inflammatory	138:149	arg1	disease					157:163	an inflammatory bowel disease	135:163	an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis	135:299	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35132375	1	55	theme	rectum	228:233	arg1	membrane					202:209	the mucous membrane	191:209	the mucous membrane of the colon and rectum	191:233	Ulcerative colitis is an inflammatory bowel disease that forms ulcerations in the mucous membrane of the colon and rectum, in which gut microbiota plays a pivotal role in its pathogenesis.
35630762	0	0	theme	CRPV	88:91	arg1	Specifications					70:83	Different Medicinal Specifications	50:83	Different Medicinal Specifications of CRPV	50:91	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	4	1	from	differences	647:657	arg1	color					675:679	their powder color	662:679	their powder color	662:679	Both showed significant differences in their powder color and microscopic characteristics.
35630762	4	1	from	differences	647:657	arg1	characteristics					697:711	microscopic characteristics	685:711	microscopic characteristics	685:711	Both showed significant differences in their powder color and microscopic characteristics.
35630762	5	2	theme	acids	830:834	arg1	absorption					805:814	the C=O peak absorption	792:814	the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP	792:868	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	2	theme	acids	830:834	arg1	higher					874:879	higher	874:879	higher	874:879	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	7	3	theme	large	1074:1078	arg1	differences					1089:1099	large distinct differences	1074:1099	large distinct differences	1074:1099	GQP and SHQP had large distinct differences.
35630762	5	4	theme	bending	928:934	arg1	peaks					936:940	the C-OH and C-H plane bending peaks	905:940	the C-OH and C-H plane bending peaks of polysaccharides	905:959	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	4	theme	bending	928:934	arg1	lower					966:970	lower	966:970	lower	966:970	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	8	5	theme	mass	1115:1118	arg1	measurements					1120:1131	the mass measurements	1111:1131	the mass measurements for molecular and characteristic fragment ions	1111:1178	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	1	6	theme	Citrus	257:262	arg1	Blanco					275:280	Citrus reticulata Blanco	257:280	Citrus reticulata Blanco	257:280	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	7	7	theme	distinct	1080:1087	arg1	differences					1089:1099	large distinct differences	1074:1099	large distinct differences	1074:1099	GQP and SHQP had large distinct differences.
35630762	8	8	from	CRPV	1221:1224	arg1	constituents					1203:1214	44 main constituents	1195:1214	44 main constituents	1195:1214	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	8	8	from	CRPV	1221:1224	arg1	glycosides					1257:1266	different flavonoid glycosides	1237:1266	different flavonoid glycosides	1237:1266	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	8	8	from	CRPV	1221:1224	arg1	aglycones					1282:1290	flavonoid aglycones	1272:1290	flavonoid aglycones	1272:1290	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	10	9	dep	polymethoxyflavone	1587:1604	arg1	nobiletin					1607:1615	nobiletin	1607:1615	nobiletin	1607:1615	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	10	9	dep	polymethoxyflavone	1587:1604	arg1	etc.					1630:1633	etc.	1630:1633	etc.	1630:1633	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	10	9	dep	polymethoxyflavone	1587:1604	arg1	tangeretin					1618:1627	tangeretin	1618:1627	tangeretin	1618:1627	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	3	10	theme	ultra-high-performance	496:517	arg1	chromatography					526:539	ultra-high-performance liquid chromatography	496:539	ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS)	496:620	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	1	11	theme	reticulata	264:273	arg1	Blanco					275:280	Citrus reticulata Blanco	257:280	Citrus reticulata Blanco	257:280	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	11	12	theme	future	1725:1730	arg1	research					1748:1755	future pharmacological research	1725:1755	future pharmacological research	1725:1755	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	10	13	theme	glycoside	1512:1520	arg1	content					1552:1558	a higher flavonoid glycoside (narirutin, hesperidin, etc.) content	1493:1558	a higher flavonoid glycoside (narirutin, hesperidin, etc.) content	1493:1558	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	5	14	theme	Fourier-transform	714:730	arg1	results					772:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	11	15	theme	precision	1887:1895	arg1	medicine					1897:1904	a precision medicine	1885:1904	a precision medicine	1885:1904	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	3	16	theme	quadrupole-orbitrap	553:571	arg1	spectrometry					578:589	hybrid quadrupole-orbitrap mass spectrometry	546:589	hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS)	546:620	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	3	16	theme	quadrupole-orbitrap	553:571	arg1	Orbitrap-MS					609:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	1	17	theme	Blanco	275:280	arg1	product					246:252	the processed product	232:252	the processed product of Citrus reticulata Blanco	232:280	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	1	17	theme	Blanco	275:280	arg1	Viride					215:220	Citri Reticulatae Pericarpium Viride	185:220	Citri Reticulatae Pericarpium Viride (CRPV)	185:227	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	5	18	theme	C=O	796:798	arg1	absorption					805:814	the C=O peak absorption	792:814	the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP	792:868	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	18	theme	C=O	796:798	arg1	higher					874:879	higher	874:879	higher	874:879	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	3	19	with	chromatography	526:539	arg1	spectrometry					578:589	hybrid quadrupole-orbitrap mass spectrometry	546:589	hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS)	546:620	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	3	19	with	chromatography	526:539	arg1	Orbitrap-MS					609:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	3	20	theme	mass	573:576	arg1	spectrometry					578:589	hybrid quadrupole-orbitrap mass spectrometry	546:589	hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS)	546:620	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	3	20	theme	mass	573:576	arg1	Orbitrap-MS					609:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	8	21	theme	molecular	1137:1145	arg1	ions					1175:1178	molecular and characteristic fragment ions	1137:1178	molecular and characteristic fragment ions	1137:1178	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	0	22	theme	Multiple	103:110	arg1	Techniques					125:134	Multiple Instrumental Techniques	103:134	Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis	103:182	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	4	23	theme	powder	668:673	arg1	color					675:679	their powder color	662:679	their powder color	662:679	Both showed significant differences in their powder color and microscopic characteristics.
35630762	5	24	theme	C-OH	909:912	arg1	peaks					936:940	the C-OH and C-H plane bending peaks	905:940	the C-OH and C-H plane bending peaks of polysaccharides	905:959	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	24	theme	C-OH	909:912	arg1	lower					966:970	lower	966:970	lower	966:970	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	1	25	theme	Citri	185:189	arg1	product					246:252	the processed product	232:252	the processed product of Citrus reticulata Blanco	232:280	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	1	25	theme	Citri	185:189	arg1	CRPV					223:226	CRPV	223:226	CRPV	223:226	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	1	25	theme	Citri	185:189	arg1	Viride					215:220	Citri Reticulatae Pericarpium Viride	185:220	Citri Reticulatae Pericarpium Viride (CRPV)	185:227	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	3	26	theme	hybrid	546:551	arg1	spectrometry					578:589	hybrid quadrupole-orbitrap mass spectrometry	546:589	hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS)	546:620	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	3	26	theme	hybrid	546:551	arg1	Orbitrap-MS					609:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	5	27	theme	peak	800:803	arg1	absorption					805:814	the C=O peak absorption	792:814	the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP	792:868	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	27	theme	peak	800:803	arg1	higher					874:879	higher	874:879	higher	874:879	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	8	28	theme	main	1198:1201	arg1	constituents					1203:1214	44 main constituents	1195:1214	44 main constituents	1195:1214	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	8	28	theme	main	1198:1201	arg1	glycosides					1257:1266	different flavonoid glycosides	1237:1266	different flavonoid glycosides	1237:1266	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	8	28	theme	main	1198:1201	arg1	aglycones					1282:1290	flavonoid aglycones	1272:1290	flavonoid aglycones	1272:1290	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	5	29	theme	analysis	763:770	arg1	results					772:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	10	30	theme	higher	1580:1585	arg1	content					1636:1642	a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content	1578:1642	a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content	1578:1642	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	3	31	theme	UHPLC-Q-Exactive	592:607	arg1	spectrometry					578:589	hybrid quadrupole-orbitrap mass spectrometry	546:589	hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS)	546:620	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	3	31	theme	UHPLC-Q-Exactive	592:607	arg1	Orbitrap-MS					609:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	UHPLC-Q-Exactive Orbitrap-MS	592:619	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	5	32	theme	FT-IR	742:746	arg1	results					772:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	1	33	theme	Pericarpium	203:213	arg1	product					246:252	the processed product	232:252	the processed product of Citrus reticulata Blanco	232:280	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	1	33	theme	Pericarpium	203:213	arg1	CRPV					223:226	CRPV	223:226	CRPV	223:226	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	1	33	theme	Pericarpium	203:213	arg1	Viride					215:220	Citri Reticulatae Pericarpium Viride	185:220	Citri Reticulatae Pericarpium Viride (CRPV)	185:227	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	2	34	theme	chemical	421:428	arg1	composition					430:440	chemical composition	421:440	chemical composition	421:440	We systematically analyzed two CRPV types, Geqingpi (GQP) and Sihuaqingpi (SHQP), based on powder color, microscopic characteristics, and chemical composition.
35630762	11	35	theme	pharmacological	1732:1746	arg1	research					1748:1755	future pharmacological research	1725:1755	future pharmacological research	1725:1755	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	10	36	theme	flavonoid	1502:1510	arg1	glycoside					1512:1520	a higher flavonoid glycoside	1493:1520	a higher flavonoid glycoside (narirutin, hesperidin, etc.) content	1493:1558	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	8	37	theme	fragment	1166:1173	arg1	ions					1175:1178	molecular and characteristic fragment ions	1137:1178	molecular and characteristic fragment ions	1137:1178	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	7	38	contain	had	1070:1072	arg1	GQP					1057:1059	GQP	1057:1059	GQP	1057:1059	GQP and SHQP had large distinct differences.
35630762	7	38	contain	had	1070:1072	arg2	differences					1089:1099	large distinct differences	1074:1099	large distinct differences	1074:1099	GQP and SHQP had large distinct differences.
35630762	7	38	contain	had	1070:1072	arg1	SHQP					1065:1068	SHQP	1065:1068	SHQP	1065:1068	GQP and SHQP had large distinct differences.
35630762	11	39	theme	scientific	1777:1786	arg1	basis					1788:1792	a scientific basis	1775:1792	a scientific basis for formulating more reasonable CRPV quality standards	1775:1847	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	0	40	theme	Techniques	125:134	arg1	Use					96:98	Use	96:98	Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis	96:182	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	10	41	theme	peak	1452:1455	arg1	measurements					1462:1473	The peak area measurements	1448:1473	The peak area measurements	1448:1473	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	5	42	theme	esters	855:860	arg1	absorption					805:814	the C=O peak absorption	792:814	the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP	792:868	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	42	theme	esters	855:860	arg1	higher					874:879	higher	874:879	higher	874:879	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	10	43	theme	area	1457:1460	arg1	measurements					1462:1473	The peak area measurements	1448:1473	The peak area measurements	1448:1473	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	0	44	theme	Physical	12:19	arg1	Compositions					34:45	Physical and Chemical Compositions	12:45	Physical and Chemical Compositions of Different Medicinal Specifications of CRPV	12:91	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	0	45	theme	Instrumental	112:123	arg1	Techniques					125:134	Multiple Instrumental Techniques	103:134	Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis	103:182	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	11	46	theme	CRPV	1680:1683	arg1	types					1685:1689	two CRPV types	1676:1689	two CRPV types	1676:1689	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	2	47	theme	CRPV	314:317	arg1	types					319:323	two CRPV types	310:323	two CRPV types	310:323	We systematically analyzed two CRPV types, Geqingpi (GQP) and Sihuaqingpi (SHQP), based on powder color, microscopic characteristics, and chemical composition.
35630762	2	47	theme	CRPV	314:317	arg1	Sihuaqingpi					345:355	Sihuaqingpi	345:355	Sihuaqingpi (SHQP)	345:362	We systematically analyzed two CRPV types, Geqingpi (GQP) and Sihuaqingpi (SHQP), based on powder color, microscopic characteristics, and chemical composition.
35630762	2	47	theme	CRPV	314:317	arg1	Geqingpi					326:333	Geqingpi	326:333	Geqingpi (GQP)	326:339	We systematically analyzed two CRPV types, Geqingpi (GQP) and Sihuaqingpi (SHQP), based on powder color, microscopic characteristics, and chemical composition.
35630762	8	48	theme	characteristic	1151:1164	arg1	ions					1175:1178	molecular and characteristic fragment ions	1137:1178	molecular and characteristic fragment ions	1137:1178	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	2	49	theme	microscopic	388:398	arg1	characteristics					400:414	microscopic characteristics	388:414	microscopic characteristics	388:414	We systematically analyzed two CRPV types, Geqingpi (GQP) and Sihuaqingpi (SHQP), based on powder color, microscopic characteristics, and chemical composition.
35630762	0	50	theme	Compositions	34:45	arg1	Analyses					0:7	Analyses	0:7	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.	0:183	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	5	51	from	absorption	805:814	arg1	SHQP					865:868	SHQP	865:868	SHQP	865:868	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	10	52	contain	had	1574:1576	arg2	content					1636:1642	a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content	1578:1642	a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content	1578:1642	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	10	52	contain	had	1574:1576	arg1	SHQP					1569:1572	SHQP	1569:1572	SHQP	1569:1572	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	11	53	theme	clinical	1863:1870	arg1	potential					1872:1880	its clinical potential	1859:1880	its clinical potential as a precision medicine	1859:1904	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	4	54	theme	microscopic	685:695	arg1	characteristics					697:711	microscopic characteristics	685:711	microscopic characteristics	685:711	Both showed significant differences in their powder color and microscopic characteristics.
35630762	2	55	theme	powder	374:379	arg1	color					381:385	powder color	374:385	powder color	374:385	We systematically analyzed two CRPV types, Geqingpi (GQP) and Sihuaqingpi (SHQP), based on powder color, microscopic characteristics, and chemical composition.
35630762	0	56	theme	Chemical	25:32	arg1	Compositions					34:45	Physical and Chemical Compositions	12:45	Physical and Chemical Compositions of Different Medicinal Specifications of CRPV	12:91	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	6	57	theme	similarity	1018:1027	arg1	analysis					1029:1036	similarity analysis	1018:1036	similarity analysis	1018:1036	We analyzed these data via similarity analysis, PCA, and OPLS-DA.
35630762	10	58	dep	glycoside	1512:1520	arg1	etc.					1546:1549	etc.	1546:1549	etc.	1546:1549	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	10	58	dep	glycoside	1512:1520	arg1	hesperidin					1534:1543	hesperidin	1534:1543	hesperidin	1534:1543	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	10	58	dep	glycoside	1512:1520	arg1	narirutin					1523:1531	narirutin	1523:1531	narirutin	1523:1531	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	10	59	dep	showed	1475:1480	arg1	whereas					1561:1567	whereas	1561:1567	whereas	1561:1567	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	0	60	theme	Different	50:58	arg1	Specifications					70:83	Different Medicinal Specifications	50:83	Different Medicinal Specifications of CRPV	50:91	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	5	61	theme	C-H	918:920	arg1	bending					928:934	C-H plane bending	918:934	C-H plane bending	918:934	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	0	62	theme	Statistical	163:173	arg1	Analysis					175:182	Multivariate Statistical Analysis	150:182	Multivariate Statistical Analysis	150:182	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	5	63	theme	carboxylic	819:828	arg1	acids					830:834	carboxylic acids	819:834	carboxylic acids	819:834	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	1	64	dep	Citri	185:189	arg1	Reticulatae					191:201	Reticulatae	191:201	Reticulatae	191:201	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	10	65	theme	polymethoxyflavone	1587:1604	arg1	content					1636:1642	a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content	1578:1642	a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content	1578:1642	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	11	66	theme	quality	1831:1837	arg1	standards					1839:1847	more reasonable CRPV quality standards	1810:1847	more reasonable CRPV quality standards	1810:1847	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	4	67	theme	significant	635:645	arg1	differences					647:657	significant differences	635:657	significant differences in their powder color and microscopic characteristics	635:711	Both showed significant differences in their powder color and microscopic characteristics.
35630762	0	68	theme	Multivariate	150:161	arg1	Analysis					175:182	Multivariate Statistical Analysis	150:182	Multivariate Statistical Analysis	150:182	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	5	69	theme	polysaccharides	945:959	arg1	peaks					936:940	the C-OH and C-H plane bending peaks	905:940	the C-OH and C-H plane bending peaks of polysaccharides	905:959	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	69	theme	polysaccharides	945:959	arg1	lower					966:970	lower	966:970	lower	966:970	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	8	70	theme	flavonoid	1272:1280	arg1	aglycones					1282:1290	flavonoid aglycones	1272:1290	flavonoid aglycones	1272:1290	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	10	71	theme	higher	1495:1500	arg1	glycoside					1512:1520	a higher flavonoid glycoside	1493:1520	a higher flavonoid glycoside (narirutin, hesperidin, etc.) content	1493:1558	The peak area measurements showed GQP having a higher flavonoid glycoside (narirutin, hesperidin, etc.) content, whereas SHQP had a higher polymethoxyflavone (nobiletin, tangeretin, etc.) content.
35630762	5	72	theme	spectroscopic	749:761	arg1	results					772:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	11	73	theme	CRPV	1826:1829	arg1	standards					1839:1847	more reasonable CRPV quality standards	1810:1847	more reasonable CRPV quality standards	1810:1847	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
35630762	8	74	theme	different	1237:1245	arg1	glycosides					1257:1266	different flavonoid glycosides	1237:1266	different flavonoid glycosides	1237:1266	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	1	75	theme	processed	236:244	arg1	product					246:252	the processed product	232:252	the processed product of Citrus reticulata Blanco	232:280	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	1	75	theme	processed	236:244	arg1	Viride					215:220	Citri Reticulatae Pericarpium Viride	185:220	Citri Reticulatae Pericarpium Viride (CRPV)	185:227	Citri Reticulatae Pericarpium Viride (CRPV) is the processed product of Citrus reticulata Blanco.
35630762	0	76	theme	Specifications	70:83	arg1	Compositions					34:45	Physical and Chemical Compositions	12:45	Physical and Chemical Compositions of Different Medicinal Specifications of CRPV	12:91	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	3	77	theme	liquid	519:524	arg1	chromatography					526:539	ultra-high-performance liquid chromatography	496:539	ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS)	496:620	In addition, we characterized their constituents via ultra-high-performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS).
35630762	8	78	theme	flavonoid	1247:1255	arg1	glycosides					1257:1266	different flavonoid glycosides	1237:1266	different flavonoid glycosides	1237:1266	Based on the mass measurements for molecular and characteristic fragment ions, we identified 44 main constituents from CRPV, including different flavonoid glycosides and flavonoid aglycones in SHQP and GQP, respectively.
35630762	5	79	theme	infrared	732:739	arg1	results					772:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results	714:778	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	0	80	theme	Medicinal	60:68	arg1	Specifications					70:83	Different Medicinal Specifications	50:83	Different Medicinal Specifications of CRPV	50:91	Analyses of Physical and Chemical Compositions of Different Medicinal Specifications of CRPV by Use of Multiple Instrumental Techniques Combined with Multivariate Statistical Analysis.
35630762	5	81	theme	carbonyl	846:853	arg1	esters					855:860	their carbonyl esters	840:860	their carbonyl esters	840:860	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	5	82	theme	plane	922:926	arg1	bending					928:934	C-H plane bending	918:934	C-H plane bending	918:934	Fourier-transform infrared (FT-IR) spectroscopic analysis results showed that the C=O peak absorption of carboxylic acids and their carbonyl esters in SHQP was higher than that of GQP, while the C-OH and C-H plane bending peaks of polysaccharides were lower than those of GQP.
35630762	11	83	theme	reasonable	1815:1824	arg1	standards					1839:1847	more reasonable CRPV quality standards	1810:1847	more reasonable CRPV quality standards	1810:1847	Since we holistically analyzed two CRPV types, the results can not only support future pharmacological research, but also provide a scientific basis for formulating more reasonable CRPV quality standards and guide its clinical potential as a precision medicine.
36558381	6	0	theme	hepatocytes	1009:1019	arg1	damage					999:1004	the damage	995:1004	the damage of hepatocytes	995:1019	This suggests that PSS could reduce the content of blood lipids and initially improve the damage of hepatocytes.
36558381	3	1	contain	has	398:400	arg2	effects					425:431	potential hypoglycemic effects	402:431	potential hypoglycemic effects	402:431	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	3	1	contain	has	398:400	arg1	PSS					393:395	PSS	393:395	PSS	393:395	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	3	1	contain	has	398:400	arg1	saponin					384:390	Polygonatum sibiricum saponin	362:390	Polygonatum sibiricum saponin (PSS)	362:396	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	4	2	theme	organ	575:579	arg1	index					581:585	the organ index	571:585	the organ index	571:585	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	1	3	theme	insulin	279:285	arg1	resistance					287:296	insulin resistance	279:296	insulin resistance	279:296	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	9	4	theme	glycogen	1531:1538	arg1	synthase					1540:1547	glycogen synthase	1531:1547	glycogen synthase (GS)	1531:1552	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	4	theme	glycogen	1531:1538	arg1	GS					1550:1551	GS	1550:1551	GS	1550:1551	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	0	5	theme	Polygonatum	0:10	arg1	saponin					22:28	Polygonatum sibiricum saponin	0:28	Polygonatum sibiricum saponin	0:28	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	11	6	dep	potential	1966:1974	arg1	new					1976:1978	new	1976:1978	new	1976:1978	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	11	7	theme	excellent	1918:1926	arg1	effect					1941:1946	an excellent hypoglycemic effect	1915:1946	an excellent hypoglycemic effect	1915:1946	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	8	8	theme	glucose	1186:1192	arg1	tolerance					1194:1202	oral glucose tolerance	1181:1202	oral glucose tolerance	1181:1202	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	5	9	theme	total	680:684	arg1	TCHO					699:702	TCHO	699:702	TCHO	699:702	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	9	theme	total	680:684	arg1	cholesterol					686:696	total cholesterol	680:696	total cholesterol (TCHO)	680:703	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	1	10	theme	systemic	211:218	arg1	disorder					230:237	a systemic metabolic disorder	209:237	a systemic metabolic disorder characterized by insulin deficiency and insulin resistance	209:296	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	1	10	theme	systemic	211:218	arg1	mellitus					190:197	Type 2 diabetes mellitus	174:197	Type 2 diabetes mellitus (T2DM)	174:204	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	9	11	theme	hepatic	1574:1580	arg1	synthesis					1591:1599	hepatic glycogen synthesis	1574:1599	hepatic glycogen synthesis	1574:1599	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	10	12	theme	important	1842:1850	arg1	bacteria					1852:1859	important bacteria	1842:1859	important bacteria	1842:1859	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	5	13	theme	total	655:659	arg1	triglyceride					661:672	total triglyceride	655:672	total triglyceride (TG)	655:677	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	13	theme	total	655:659	arg1	TG					675:676	TG	675:676	TG	675:676	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	9	14	theme	B	1399:1399	arg1	content					1358:1364	the content	1354:1364	the content of phosphorylated protein kinase B (AKT)	1354:1405	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	8	15	theme	serum	1215:1219	arg1	LPS					1241:1243	LPS	1241:1243	LPS	1241:1243	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	8	15	theme	serum	1215:1219	arg1	lipopolysaccharide					1221:1238	serum lipopolysaccharide	1215:1238	serum lipopolysaccharide (LPS)	1215:1244	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	5	16	theme	low-density	706:716	arg1	LDL-C					743:747	LDL-C	743:747	LDL-C	743:747	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	16	theme	low-density	706:716	arg1	cholesterol					730:740	low-density lipoprotein cholesterol	706:740	low-density lipoprotein cholesterol (LDL-C)	706:748	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	10	17	theme	bacteria	1668:1675	arg1	growth					1647:1652	the growth	1643:1652	the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus	1643:1717	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	0	18	theme	Diabetes	79:86	arg1	Mice					88:91	Type 2 Diabetes Mice	72:91	Type 2 Diabetes Mice	72:91	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	3	19	theme	Polygonatum	362:372	arg1	PSS					393:395	PSS	393:395	PSS	393:395	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	3	19	theme	Polygonatum	362:372	arg1	saponin					384:390	Polygonatum sibiricum saponin	362:390	Polygonatum sibiricum saponin (PSS)	362:396	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	8	20	theme	glycosylated	1250:1261	arg1	hemoglobin					1263:1272	glycosylated hemoglobin	1250:1272	glycosylated hemoglobin (HbA1c) levels in T2DM mice	1250:1300	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	8	20	theme	glycosylated	1250:1261	arg1	HbA1c					1275:1279	HbA1c	1275:1279	HbA1c	1275:1279	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	0	21	theme	Hepatic	106:112	arg1	Resistance					122:131	Hepatic Insulin Resistance	106:131	Hepatic Insulin Resistance	106:131	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	9	22	theme	glucose	1440:1446	arg1	transporter					1448:1458	glucose transporter 4	1440:1460	glucose transporter 4 (GLUT-4)	1440:1469	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	22	theme	glucose	1440:1446	arg1	GLUT-4					1463:1468	GLUT-4	1463:1468	GLUT-4	1463:1468	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	11	23	theme	in-depth	2021:2028	arg1	research					2030:2037	more in-depth research	2016:2037	more in-depth research	2016:2037	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	5	24	theme	aminotransferase	759:774	arg1	content					644:650	the content	640:650	the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood	640:831	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	25	theme	high-density	863:874	arg1	HDL-C					901:905	HDL-C	901:905	HDL-C	901:905	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	25	theme	high-density	863:874	arg1	cholesterol					888:898	high-density lipoprotein cholesterol	863:898	high-density lipoprotein cholesterol (HDL-C)	863:906	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	4	26	theme	glucose	544:550	arg1	level					529:533	the level	525:533	the level of blood glucose, water intake, and the organ index in diabetic mice	525:602	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	3	27	theme	further	467:473	arg1	study					475:479	further study	467:479	further study	467:479	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	7	28	theme	islet	1088:1092	arg1	cells					1099:1103	islet beta cells	1088:1103	islet beta cells	1088:1103	We found that PSS alleviated hepatic insulin resistance, repaired islet beta cells, and enabled insulin to play its biological role normally.
36558381	5	29	theme	cholesterol	888:898	arg1	content					852:858	the content	848:858	the content of high-density lipoprotein cholesterol (HDL-C)	848:906	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	6	30	theme	lipids	966:971	arg1	content					949:955	the content	945:955	the content of blood lipids	945:971	This suggests that PSS could reduce the content of blood lipids and initially improve the damage of hepatocytes.
36558381	3	31	theme	hypoglycemic	412:423	arg1	effects					425:431	potential hypoglycemic effects	402:431	potential hypoglycemic effects	402:431	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	4	32	theme	water	553:557	arg1	intake					559:564	water intake	553:564	water intake	553:564	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	9	33	dep	increased	1344:1352	arg1	activating					1476:1485	activating	1476:1485	activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis	1476:1599	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	33	dep	increased	1344:1352	arg1	promoting					1416:1424	promoting	1416:1424	promoting the effect of glucose transporter 4 (GLUT-4)	1416:1469	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	5	34	theme	aminotransferase	797:812	arg1	content					644:650	the content	640:650	the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood	640:831	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	0	35	theme	Beneficial	37:46	arg1	Effects					61:67	Beneficial Hypoglycemic Effects	37:67	Beneficial Hypoglycemic Effects	37:67	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	7	36	theme	biological	1138:1147	arg1	role					1149:1152	its biological role	1134:1152	its biological role	1134:1152	We found that PSS alleviated hepatic insulin resistance, repaired islet beta cells, and enabled insulin to play its biological role normally.
36558381	2	37	theme	public	347:352	arg1	health					354:359	public health	347:359	public health	347:359	Recently, it has become a significant threat to public health.
36558381	7	38	theme	insulin	1059:1065	arg1	resistance					1067:1076	hepatic insulin resistance	1051:1076	hepatic insulin resistance	1051:1076	We found that PSS alleviated hepatic insulin resistance, repaired islet beta cells, and enabled insulin to play its biological role normally.
36558381	1	39	theme	metabolic	220:228	arg1	disorder					230:237	a systemic metabolic disorder	209:237	a systemic metabolic disorder characterized by insulin deficiency and insulin resistance	209:296	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	1	39	theme	metabolic	220:228	arg1	mellitus					190:197	Type 2 diabetes mellitus	174:197	Type 2 diabetes mellitus (T2DM)	174:204	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	9	40	theme	synthase	1496:1503	arg1	GSK-3β					1519:1524	GSK-3β	1519:1524	GSK-3β	1519:1524	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	40	theme	synthase	1496:1503	arg1	3beta					1512:1516	glycogen synthase kinase 3beta	1487:1516	glycogen synthase kinase 3beta (GSK-3β)	1487:1525	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	4	41	theme	index	581:585	arg1	level					529:533	the level	525:533	the level of blood glucose, water intake, and the organ index in diabetic mice	525:602	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	11	42	dep	has	1911:1913	arg1	provides					1955:1962	provides	1955:1962	provides a potential new treatment for T2DM and guidance for more in-depth research	1955:2037	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	4	43	from	level	529:533	arg1	mice					599:602	diabetic mice	590:602	diabetic mice	590:602	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	9	44	theme	3beta	1512:1516	arg1	proteins					1554:1561	glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins	1487:1561	glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis	1487:1599	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	4	45	theme	diabetic	590:597	arg1	mice					599:602	diabetic mice	590:602	diabetic mice	590:602	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	8	46	from	levels	1282:1287	arg1	mice					1297:1300	T2DM mice	1292:1300	T2DM mice	1292:1300	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	1	47	theme	insulin	256:262	arg1	deficiency					264:273	insulin deficiency	256:273	insulin deficiency	256:273	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	10	48	theme	bacteria	1749:1756	arg1	growth					1731:1736	the growth	1727:1736	the growth of harmful bacteria such as Enterococcus and Enterobacter	1727:1794	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	11	49	theme	potential	1966:1974	arg1	treatment					1980:1988	a potential new treatment	1964:1988	a potential new treatment for T2DM and guidance for more in-depth research	1964:2037	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	5	50	theme	alanine	751:757	arg1	aminotransferase					759:774	alanine aminotransferase	751:774	alanine aminotransferase (ALT)	751:780	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	50	theme	alanine	751:757	arg1	ALT					777:779	ALT	777:779	ALT	777:779	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	1	51	theme	diabetes	181:188	arg1	T2DM					200:203	T2DM	200:203	T2DM	200:203	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	1	51	theme	diabetes	181:188	arg1	mellitus					190:197	Type 2 diabetes mellitus	174:197	Type 2 diabetes mellitus (T2DM)	174:204	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	1	51	theme	diabetes	181:188	arg1	disorder					230:237	a systemic metabolic disorder	209:237	a systemic metabolic disorder characterized by insulin deficiency and insulin resistance	209:296	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	3	52	theme	potential	402:410	arg1	effects					425:431	potential hypoglycemic effects	402:431	potential hypoglycemic effects	402:431	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	9	53	theme	synthase	1540:1547	arg1	proteins					1554:1561	glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins	1487:1561	glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis	1487:1599	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	5	54	theme	cholesterol	730:740	arg1	content					644:650	the content	640:650	the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood	640:831	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	11	55	theme	hypoglycemic	1928:1939	arg1	effect					1941:1946	an excellent hypoglycemic effect	1915:1946	an excellent hypoglycemic effect	1915:1946	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	0	56	theme	Synthesis-Related	146:162	arg1	Proteins					164:171	Glycogen Synthesis-Related Proteins	137:171	Glycogen Synthesis-Related Proteins	137:171	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	10	57	theme	beneficial	1657:1666	arg1	Lactobacillus					1705:1717	Lactobacillus	1705:1717	Lactobacillus	1705:1717	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	10	57	theme	beneficial	1657:1666	arg1	bacteria					1668:1675	beneficial bacteria	1657:1675	beneficial bacteria such as Bifidobacterium and Lactobacillus	1657:1717	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	10	57	theme	beneficial	1657:1666	arg1	Bifidobacterium					1685:1699	Bifidobacterium	1685:1699	Bifidobacterium	1685:1699	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	9	58	theme	phosphorylated	1369:1382	arg1	AKT					1402:1404	AKT	1402:1404	AKT	1402:1404	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	58	theme	phosphorylated	1369:1382	arg1	B					1399:1399	phosphorylated protein kinase B	1369:1399	phosphorylated protein kinase B (AKT)	1369:1405	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	8	59	theme	oral	1181:1184	arg1	tolerance					1194:1202	oral glucose tolerance	1181:1202	oral glucose tolerance	1181:1202	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	10	60	theme	bacteria	1852:1859	arg1	composition					1827:1837	the composition	1823:1837	the composition of important bacteria in the intestine	1823:1876	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	9	61	theme	kinase	1392:1397	arg1	AKT					1402:1404	AKT	1402:1404	AKT	1402:1404	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	61	theme	kinase	1392:1397	arg1	B					1399:1399	phosphorylated protein kinase B	1369:1399	phosphorylated protein kinase B (AKT)	1369:1405	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	62	theme	glycogen	1582:1589	arg1	synthesis					1591:1599	hepatic glycogen synthesis	1574:1599	hepatic glycogen synthesis	1574:1599	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	5	63	theme	triglyceride	661:672	arg1	content					644:650	the content	640:650	the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood	640:831	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	11	64	contain	has	1911:1913	arg2	effect					1941:1946	an excellent hypoglycemic effect	1915:1946	an excellent hypoglycemic effect	1915:1946	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	11	64	contain	has	1911:1913	arg1	PSS					1907:1909	PSS	1907:1909	PSS	1907:1909	These studies indicate that PSS has an excellent hypoglycemic effect, which provides a potential new treatment for T2DM and guidance for more in-depth research.
36558381	5	65	theme	lipoprotein	718:728	arg1	LDL-C					743:747	LDL-C	743:747	LDL-C	743:747	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	65	theme	lipoprotein	718:728	arg1	cholesterol					730:740	low-density lipoprotein cholesterol	706:740	low-density lipoprotein cholesterol (LDL-C)	706:748	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	1	66	theme	Type	174:177	arg1	T2DM					200:203	T2DM	200:203	T2DM	200:203	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	1	66	theme	Type	174:177	arg1	mellitus					190:197	Type 2 diabetes mellitus	174:197	Type 2 diabetes mellitus (T2DM)	174:204	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	1	66	theme	Type	174:177	arg1	disorder					230:237	a systemic metabolic disorder	209:237	a systemic metabolic disorder characterized by insulin deficiency and insulin resistance	209:296	Type 2 diabetes mellitus (T2DM) is a systemic metabolic disorder characterized by insulin deficiency and insulin resistance.
36558381	5	67	theme	cholesterol	686:696	arg1	content					644:650	the content	640:650	the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood	640:831	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	10	68	from	composition	1827:1837	arg1	intestine					1868:1876	the intestine	1864:1876	the intestine	1864:1876	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	3	69	theme	sibiricum	374:382	arg1	PSS					393:395	PSS	393:395	PSS	393:395	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	3	69	theme	sibiricum	374:382	arg1	saponin					384:390	Polygonatum sibiricum saponin	362:390	Polygonatum sibiricum saponin (PSS)	362:396	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	0	70	theme	Insulin	114:120	arg1	Resistance					122:131	Hepatic Insulin Resistance	106:131	Hepatic Insulin Resistance	106:131	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	8	71	theme	hemoglobin	1263:1272	arg1	levels					1282:1287	glycosylated hemoglobin (HbA1c) levels	1250:1287	glycosylated hemoglobin (HbA1c) levels in T2DM mice	1250:1300	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	3	72	theme	specific	442:449	arg1	mechanism					451:459	its specific mechanism	438:459	its specific mechanism	438:459	Polygonatum sibiricum saponin (PSS) has potential hypoglycemic effects, but its specific mechanism needs further study.
36558381	0	73	theme	sibiricum	12:20	arg1	saponin					22:28	Polygonatum sibiricum saponin	0:28	Polygonatum sibiricum saponin	0:28	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	9	74	theme	transporter	1448:1458	arg1	effect					1430:1435	the effect	1426:1435	the effect of glucose transporter 4 (GLUT-4)	1426:1469	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	8	75	gly	glycosylated	1250:1261	arg1	hemoglobin					1263:1272	glycosylated hemoglobin	1250:1272	glycosylated hemoglobin (HbA1c) levels in T2DM mice	1250:1300	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	8	75	gly	glycosylated	1250:1261	arg1	HbA1c					1275:1279	HbA1c	1275:1279	HbA1c	1275:1279	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	8	76	from	lipopolysaccharide	1221:1238	arg1	mice					1297:1300	T2DM mice	1292:1300	T2DM mice	1292:1300	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	5	77	theme	lipoprotein	876:886	arg1	HDL-C					901:905	HDL-C	901:905	HDL-C	901:905	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	77	theme	lipoprotein	876:886	arg1	cholesterol					888:898	high-density lipoprotein cholesterol	863:898	high-density lipoprotein cholesterol (HDL-C)	863:906	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	4	78	theme	intake	559:564	arg1	level					529:533	the level	525:533	the level of blood glucose, water intake, and the organ index in diabetic mice	525:602	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	0	79	theme	Hypoglycemic	48:59	arg1	Effects					61:67	Beneficial Hypoglycemic Effects	37:67	Beneficial Hypoglycemic Effects	37:67	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	6	80	theme	blood	960:964	arg1	lipids					966:971	blood lipids	960:971	blood lipids	960:971	This suggests that PSS could reduce the content of blood lipids and initially improve the damage of hepatocytes.
36558381	8	81	theme	T2DM	1292:1295	arg1	mice					1297:1300	T2DM mice	1292:1300	T2DM mice	1292:1300	It also improved oral glucose tolerance and abated serum lipopolysaccharide (LPS) and glycosylated hemoglobin (HbA1c) levels in T2DM mice.
36558381	5	82	theme	aspartate	787:795	arg1	AST					815:817	AST	815:817	AST	815:817	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	5	82	theme	aspartate	787:795	arg1	aminotransferase					797:812	aspartate aminotransferase	787:812	aspartate aminotransferase (AST)	787:818	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	7	83	theme	hepatic	1051:1057	arg1	resistance					1067:1076	hepatic insulin resistance	1051:1076	hepatic insulin resistance	1051:1076	We found that PSS alleviated hepatic insulin resistance, repaired islet beta cells, and enabled insulin to play its biological role normally.
36558381	5	84	from	content	644:650	arg1	blood					827:831	the blood	823:831	the blood	823:831	Meanwhile, PSS effectively reduced the content of total triglyceride (TG), total cholesterol (TCHO), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in the blood, and increased the content of high-density lipoprotein cholesterol (HDL-C).
36558381	0	85	theme	Type	72:75	arg1	Diabetes					79:86	Type 2 Diabetes	72:86	Type 2 Diabetes Mice	72:91	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	0	86	theme	Glycogen	137:144	arg1	Proteins					164:171	Glycogen Synthesis-Related Proteins	137:171	Glycogen Synthesis-Related Proteins	137:171	Polygonatum sibiricum saponin Exerts Beneficial Hypoglycemic Effects in Type 2 Diabetes Mice by Improving Hepatic Insulin Resistance and Glycogen Synthesis-Related Proteins.
36558381	4	87	theme	blood	538:542	arg1	glucose					544:550	blood glucose	538:550	blood glucose	538:550	In this study, PSS significantly decreased the level of blood glucose, water intake, and the organ index in diabetic mice.
36558381	10	88	theme	harmful	1741:1747	arg1	Enterobacter					1783:1794	Enterobacter	1783:1794	Enterobacter	1783:1794	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	10	88	theme	harmful	1741:1747	arg1	bacteria					1749:1756	harmful bacteria	1741:1756	harmful bacteria such as Enterococcus and Enterobacter	1741:1794	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	10	88	theme	harmful	1741:1747	arg1	Enterococcus					1766:1777	Enterococcus	1766:1777	Enterococcus	1766:1777	Finally, we found that PSS could promote the growth of beneficial bacteria such as Bifidobacterium and Lactobacillus, reduce the growth of harmful bacteria such as Enterococcus and Enterobacter, and preliminarily improve the composition of important bacteria in the intestine.
36558381	9	89	theme	glycogen	1487:1494	arg1	GSK-3β					1519:1524	GSK-3β	1519:1524	GSK-3β	1519:1524	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	89	theme	glycogen	1487:1494	arg1	3beta					1512:1516	glycogen synthase kinase 3beta	1487:1516	glycogen synthase kinase 3beta (GSK-3β)	1487:1525	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	2	90	theme	significant	325:335	arg1	threat					337:342	a significant threat	323:342	a significant threat to public health	323:359	Recently, it has become a significant threat to public health.
36558381	9	91	theme	protein	1384:1390	arg1	AKT					1402:1404	AKT	1402:1404	AKT	1402:1404	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	91	theme	protein	1384:1390	arg1	B					1399:1399	phosphorylated protein kinase B	1369:1399	phosphorylated protein kinase B (AKT)	1369:1405	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	92	theme	kinase	1505:1510	arg1	GSK-3β					1519:1524	GSK-3β	1519:1524	GSK-3β	1519:1524	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	9	92	theme	kinase	1505:1510	arg1	3beta					1512:1516	glycogen synthase kinase 3beta	1487:1516	glycogen synthase kinase 3beta (GSK-3β)	1487:1525	Furthermore, studies have found that PSS increased the content of phosphorylated protein kinase B (AKT), thereby promoting the effect of glucose transporter 4 (GLUT-4), and activating glycogen synthase kinase 3beta (GSK-3β) and glycogen synthase (GS) proteins to promote hepatic glycogen synthesis.
36558381	7	93	theme	beta	1094:1097	arg1	cells					1099:1103	islet beta cells	1088:1103	islet beta cells	1088:1103	We found that PSS alleviated hepatic insulin resistance, repaired islet beta cells, and enabled insulin to play its biological role normally.
36868537	7	0	theme	oxidative	1163:1171	arg1	damage					1173:1178	oxidative damage	1163:1178	oxidative damage caused by the virus	1163:1198	HFPs maintained peroxidase activity after WSSV challenge, thereby providing protection against oxidative damage caused by the virus.
36868537	10	1	theme	peptides	1473:1480	arg1	expression					1445:1454	the expression	1441:1454	the expression of antimicrobial peptides	1441:1480	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	10	1	theme	peptides	1473:1480	arg1	phagocytosis					1512:1523	phagocytosis	1512:1523	phagocytosis	1512:1523	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	10	1	theme	peptides	1473:1480	arg1	apoptosis					1530:1538	apoptosis	1530:1538	apoptosis	1530:1538	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	10	1	theme	peptides	1473:1480	arg1	activity					1502:1509	antioxidant enzyme activity	1483:1509	antioxidant enzyme activity	1483:1509	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	3	2	dep	in	564:565	arg1	vivo					567:570	vivo	567:570	vivo	567:570	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	0	3	theme	paramamosain	110:121	arg1	resistance					76:85	disease resistance	68:85	disease resistance	68:85	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	0	3	theme	paramamosain	110:121	arg1	immunity					55:62	innate immunity	48:62	innate immunity	48:62	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	5	4	from	p53	915:917	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	5	5	theme	PCR	790:792	arg1	results					794:800	Quantitative PCR results	777:800	Quantitative PCR results	777:800	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	11	6	contain	have	1557:1560	arg2	potential					1562:1570	potential	1562:1570	potential	1562:1570	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	11	6	contain	have	1557:1560	arg1	HFPs					1552:1555	HFPs	1552:1555	HFPs	1552:1555	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	3	7	theme	in	564:565	arg1	assays					584:589	in vivo or in vitro assays	564:589	in vivo or in vitro assays	564:589	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	1	8	from	effects	222:228	arg1	response					244:251	the immune response	233:251	the immune response of the mud crab Scylla paramamosain	233:287	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	3	9	theme	immunostimulation	537:553	arg1	activity					555:562	immunostimulation activity	537:562	immunostimulation activity	537:562	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	1	10	from	fusiforme	185:193	arg1	polysaccharides					156:170	the polysaccharides	152:170	the polysaccharides from Hizikia fusiforme (HFPs)	152:200	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	5	11	theme	crustin	867:873	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	4	12	theme	spot	671:674	arg1	WSSV					692:695	WSSV	692:695	WSSV	692:695	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	4	12	theme	spot	671:674	arg1	virus					685:689	white spot syndrome virus	665:689	white spot syndrome virus (WSSV)-infected crabs	665:711	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	5	13	theme	myosin	876:881	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	2	14	theme	chain	444:448	arg1	β-type					464:469	β-type	464:469	β-type	464:469	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	2	14	theme	chain	444:448	arg1	structure					450:458	the sugar chain structure	434:458	the sugar chain structure	434:458	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	4	15	theme	viral	644:648	arg1	replication					650:660	viral replication	644:660	viral replication in white spot syndrome virus (WSSV)-infected crabs	644:711	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	3	16	dep	antioxidant	521:531	arg1	assays					584:589	in vivo or in vitro assays	564:589	in vivo or in vitro assays	564:589	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	0	17	from	Effects	0:6	arg1	resistance					76:85	disease resistance	68:85	disease resistance	68:85	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	0	17	from	Effects	0:6	arg1	immunity					55:62	innate immunity	48:62	innate immunity	48:62	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	4	18	theme	-infected	697:705	arg1	crabs					707:711	white spot syndrome virus (WSSV)-infected crabs	665:711	white spot syndrome virus (WSSV)-infected crabs	665:711	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	5	19	theme	TLR	896:898	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	0	20	theme	crab	98:101	arg1	paramamosain					110:121	the mud crab Scylla paramamosain	90:121	the mud crab Scylla paramamosain	90:121	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	5	21	theme	JAK	901:903	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	10	22	theme	innate	1393:1398	arg1	immunity					1400:1407	the innate immunity	1389:1407	the innate immunity of S. paramamosain	1389:1426	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	4	23	theme	virus	685:689	arg1	crabs					707:711	white spot syndrome virus (WSSV)-infected crabs	665:711	white spot syndrome virus (WSSV)-infected crabs	665:711	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	5	24	theme	MCM7	884:887	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	0	25	theme	Hizikia	11:17	arg1	polysaccharides					29:43	Hizikia fusiforme polysaccharides	11:43	Hizikia fusiforme polysaccharides	11:43	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	1	26	theme	mud	260:262	arg1	paramamosain					276:287	the mud crab Scylla paramamosain	256:287	the mud crab Scylla paramamosain	256:287	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	5	27	theme	STAT	890:893	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	10	28	theme	paramamosain	1415:1426	arg1	immunity					1400:1407	the innate immunity	1389:1407	the innate immunity of S. paramamosain	1389:1426	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	0	29	theme	polysaccharides	29:43	arg1	Effects					0:6	Effects	0:6	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.	0:122	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	1	30	theme	Scylla	269:274	arg1	paramamosain					276:287	the mud crab Scylla paramamosain	256:287	the mud crab Scylla paramamosain	256:287	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	2	31	theme	sulfated	404:411	arg1	HFPs					327:330	HFPs	327:330	HFPs	327:330	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	2	31	theme	sulfated	404:411	arg1	polysaccharides					413:427	sulfated polysaccharides	404:427	sulfated polysaccharides	404:427	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	10	32	theme	antimicrobial	1459:1471	arg1	peptides					1473:1480	antimicrobial peptides	1459:1480	antimicrobial peptides	1459:1480	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	0	33	theme	innate	48:53	arg1	immunity					55:62	innate immunity	48:62	innate immunity	48:62	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	6	34	theme	hemolymph	1025:1033	arg1	activities					1047:1056	the hemolymph antioxidant activities	1021:1056	the hemolymph antioxidant activities of crabs	1021:1065	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	5	35	from	STAT	890:893	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	9	36	theme	survival	1310:1317	arg1	rate					1319:1322	the survival rate	1306:1322	the survival rate of WSSV-infected crabs	1306:1345	In addition, HFPs significantly enhanced the survival rate of WSSV-infected crabs.
36868537	5	37	theme	CAP	906:908	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	10	38	theme	enzyme	1495:1500	arg1	activity					1502:1509	antioxidant enzyme activity	1483:1509	antioxidant enzyme activity	1483:1509	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	11	39	theme	mud	1651:1653	arg1	crabs					1655:1659	mud crabs	1651:1659	mud crabs	1651:1659	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	4	40	theme	Vibrio	742:747	arg1	alginolyticus					749:761	Vibrio alginolyticus	742:761	Vibrio alginolyticus	742:761	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	5	41	from	MCM7	884:887	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	6	42	theme	acid	1000:1003	arg1	phosphatase					1005:1015	acid phosphatase	1000:1015	acid phosphatase	1000:1015	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	11	43	theme	therapeutic	1583:1593	arg1	agents					1609:1614	therapeutic or preventive agents	1583:1614	agents	1609:1614	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	5	44	from	JAK	901:903	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	9	45	theme	crabs	1341:1345	arg1	rate					1319:1322	the survival rate	1306:1322	the survival rate of WSSV-infected crabs	1306:1345	In addition, HFPs significantly enhanced the survival rate of WSSV-infected crabs.
36868537	6	46	theme	dismutase	986:994	arg1	activities					961:970	the activities	957:970	the activities of superoxide dismutase and acid phosphatase	957:1015	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	6	46	theme	dismutase	986:994	arg1	activities					1047:1056	the hemolymph antioxidant activities	1021:1056	the hemolymph antioxidant activities of crabs	1021:1065	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	5	47	from	TLR	896:898	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	1	48	theme	immune	237:242	arg1	response					244:251	the immune response	233:251	the immune response of the mud crab Scylla paramamosain	233:287	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	2	49	theme	Compositional	290:302	arg1	analysis					304:311	Compositional analysis	290:311	Compositional analysis	290:311	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	11	50	theme	preventive	1598:1607	arg1	agents					1609:1614	therapeutic or preventive agents	1583:1614	agents	1609:1614	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	3	51	theme	potential	511:519	arg1	antioxidant					521:531	potential antioxidant	511:531	potential antioxidant	511:531	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	5	52	from	CAP	906:908	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	6	53	theme	crabs	1061:1065	arg1	activities					961:970	the activities	957:970	the activities of superoxide dismutase and acid phosphatase	957:1015	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	6	53	theme	crabs	1061:1065	arg1	activities					1047:1056	the hemolymph antioxidant activities	1021:1056	the hemolymph antioxidant activities of crabs	1021:1065	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	5	54	from	hemocytes	927:935	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	5	55	theme	Quantitative	777:788	arg1	results					794:800	Quantitative PCR results	777:800	Quantitative PCR results	777:800	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	5	56	theme	astakine	857:864	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	1	57	theme	Hizikia	177:183	arg1	HFPs					196:199	HFPs	196:199	HFPs	196:199	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	1	57	theme	Hizikia	177:183	arg1	fusiforme					185:193	Hizikia fusiforme	177:193	Hizikia fusiforme (HFPs)	177:200	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	3	58	dep	in	575:576	arg1	vitro					578:582	vitro	578:582	vitro	578:582	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	3	59	theme	in	575:576	arg1	assays					584:589	in vivo or in vitro assays	564:589	in vivo or in vitro assays	564:589	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	4	60	theme	white	665:669	arg1	WSSV					692:695	WSSV	692:695	WSSV	692:695	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	4	60	theme	white	665:669	arg1	virus					685:689	white spot syndrome virus	665:689	white spot syndrome virus (WSSV)-infected crabs	665:711	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	11	61	theme	microbial	1686:1694	arg1	infection					1696:1704	microbial infection	1686:1704	microbial infection	1686:1704	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	0	62	theme	disease	68:74	arg1	resistance					76:85	disease resistance	68:85	disease resistance	68:85	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	5	63	theme	expression	836:845	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	2	64	theme	sugar	438:442	arg1	β-type					464:469	β-type	464:469	β-type	464:469	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	2	64	theme	sugar	438:442	arg1	structure					450:458	the sugar chain structure	434:458	the sugar chain structure	434:458	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	8	65	theme	hemocytes	1233:1241	arg1	apoptosis					1220:1228	apoptosis	1220:1228	apoptosis of hemocytes	1220:1241	HFPs also promoted apoptosis of hemocytes after WSSV infection.
36868537	8	66	theme	WSSV	1249:1252	arg1	infection					1254:1262	WSSV infection	1249:1262	WSSV infection	1249:1262	HFPs also promoted apoptosis of hemocytes after WSSV infection.
36868537	0	67	theme	mud	94:96	arg1	paramamosain					110:121	the mud crab Scylla paramamosain	90:121	the mud crab Scylla paramamosain	90:121	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	5	68	from	astakine	857:864	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	0	69	theme	Scylla	103:108	arg1	paramamosain					110:121	the mud crab Scylla paramamosain	90:121	the mud crab Scylla paramamosain	90:121	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	5	70	from	levels	847:852	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	6	71	theme	superoxide	975:984	arg1	dismutase					986:994	superoxide dismutase	975:994	superoxide dismutase	975:994	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	7	72	theme	peroxidase	1084:1093	arg1	activity					1095:1102	peroxidase activity	1084:1102	peroxidase activity	1084:1102	HFPs maintained peroxidase activity after WSSV challenge, thereby providing protection against oxidative damage caused by the virus.
36868537	5	73	from	myosin	876:881	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	3	74	contain	have	506:509	arg2	antioxidant					521:531	potential antioxidant	511:531	potential antioxidant	511:531	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	3	74	contain	have	506:509	arg1	HFPs					501:504	HFPs	501:504	HFPs	501:504	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	3	74	contain	have	506:509	arg2	activity					555:562	immunostimulation activity	537:562	immunostimulation activity	537:562	These results indicated that HFPs have potential antioxidant and immunostimulation activity in vivo or in vitro assays.
36868537	4	75	theme	syndrome	676:683	arg1	WSSV					692:695	WSSV	692:695	WSSV	692:695	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	4	75	theme	syndrome	676:683	arg1	virus					685:689	white spot syndrome virus	665:689	white spot syndrome virus (WSSV)-infected crabs	665:711	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	2	76	theme	mannuronic	356:365	arg1	acid					367:370	mannuronic acid	356:370	mannuronic acid (49.05%)	356:379	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	2	76	theme	mannuronic	356:365	arg1	%					378:378	49.05%	373:378	49.05%	373:378	Compositional analysis revealed that HFPs were composed mainly of mannuronic acid (49.05%) and fucose (22.29%) as sulfated polysaccharides, and the sugar chain structure was β-type.
36868537	0	77	theme	fusiforme	19:27	arg1	polysaccharides					29:43	Hizikia fusiforme polysaccharides	11:43	Hizikia fusiforme polysaccharides	11:43	Effects of Hizikia fusiforme polysaccharides on innate immunity and disease resistance of the mud crab Scylla paramamosain.
36868537	5	78	from	crustin	867:873	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	11	79	theme	innate	1632:1637	arg1	immunity					1639:1646	the innate immunity	1628:1646	the innate immunity of mud crabs	1628:1659	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	1	80	theme	crab	264:267	arg1	paramamosain					276:287	the mud crab Scylla paramamosain	256:287	the mud crab Scylla paramamosain	256:287	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	5	81	theme	p53	915:917	arg1	levels					847:852	the expression levels	832:852	the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes	832:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	4	82	from	replication	650:660	arg1	crabs					707:711	white spot syndrome virus (WSSV)-infected crabs	665:711	white spot syndrome virus (WSSV)-infected crabs	665:711	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	10	83	theme	S.	1412:1413	arg1	paramamosain					1415:1426	S. paramamosain	1412:1426	S. paramamosain	1412:1426	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	4	84	theme	alginolyticus	749:761	arg1	phagocytosis					726:737	phagocytosis	726:737	phagocytosis of Vibrio alginolyticus by hemocytes	726:774	Through this research, we found that HFPs inhibited viral replication in white spot syndrome virus (WSSV)-infected crabs and promoted phagocytosis of Vibrio alginolyticus by hemocytes.
36868537	7	85	theme	WSSV	1110:1113	arg1	challenge					1115:1123	WSSV challenge	1110:1123	WSSV challenge	1110:1123	HFPs maintained peroxidase activity after WSSV challenge, thereby providing protection against oxidative damage caused by the virus.
36868537	1	86	theme	paramamosain	276:287	arg1	response					244:251	the immune response	233:251	the immune response of the mud crab Scylla paramamosain	233:287	In this study, we extracted the polysaccharides from Hizikia fusiforme (HFPs) and evaluated their effects on the immune response of the mud crab Scylla paramamosain.
36868537	5	87	theme	crab	922:925	arg1	hemocytes					927:935	crab hemocytes	922:935	crab hemocytes	922:935	Quantitative PCR results showed that HFPs up-regulated the expression levels of astakine, crustin, myosin, MCM7, STAT, TLR, JAK, CAP, and p53 in crab hemocytes.
36868537	6	88	theme	antioxidant	1035:1045	arg1	activities					1047:1056	the hemolymph antioxidant activities	1021:1056	the hemolymph antioxidant activities of crabs	1021:1065	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	11	89	theme	crabs	1655:1659	arg1	immunity					1639:1646	the innate immunity	1628:1646	the innate immunity of mud crabs	1628:1659	Therefore, HFPs have potential for use as therapeutic or preventive agents to regulate the innate immunity of mud crabs and protect them against microbial infection.
36868537	6	90	theme	phosphatase	1005:1015	arg1	activities					961:970	the activities	957:970	the activities of superoxide dismutase and acid phosphatase	957:1015	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	6	90	theme	phosphatase	1005:1015	arg1	activities					1047:1056	the hemolymph antioxidant activities	1021:1056	the hemolymph antioxidant activities of crabs	1021:1065	HFPs also promoted the activities of superoxide dismutase and acid phosphatase and the hemolymph antioxidant activities of crabs.
36868537	10	91	theme	antioxidant	1483:1493	arg1	activity					1502:1509	antioxidant enzyme activity	1483:1509	antioxidant enzyme activity	1483:1509	All results confirmed that HFPs improved the innate immunity of S. paramamosain by enhancing the expression of antimicrobial peptides, antioxidant enzyme activity, phagocytosis, and apoptosis.
36868537	9	92	theme	WSSV-infected	1327:1339	arg1	crabs					1341:1345	WSSV-infected crabs	1327:1345	WSSV-infected crabs	1327:1345	In addition, HFPs significantly enhanced the survival rate of WSSV-infected crabs.
36280015	6	0	from	macrophages	986:996	arg1	release					930:936	the LPS-induced release	914:936	the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages	914:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	2	1	theme	traditional	315:325	arg1	medicine					335:342	a traditional Chinese medicine	313:342	a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain	313:430	Its flower is a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain.
36280015	2	1	theme	traditional	315:325	arg1	flower					303:308	Its flower	299:308	Its flower	299:308	Its flower is a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain.
36280015	12	2	theme	ear	2098:2100	arg1	edema					2102:2106	xylene-induced ear edema	2083:2106	xylene-induced ear edema in mice	2083:2114	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	12	3	from	edema	2102:2106	arg1	mice					2111:2114	mice	2111:2114	mice	2111:2114	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	3	4	theme	aromatic	505:512	arg1	EO					465:466	H. flavum flower EO	448:466	H. flavum flower EO	448:466	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	3	4	theme	aromatic	505:512	arg1	stomachic					514:522	an aromatic stomachic	502:522	an aromatic stomachic	502:522	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	14	5	theme	novel	2401:2405	arg1	agent					2439:2443	a novel and promising anti-inflammatory agent	2399:2443	a novel and promising anti-inflammatory agent	2399:2443	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	6	6	theme	immunofluorescent	1078:1094	arg1	staining					1096:1103	immunofluorescent staining	1078:1103	immunofluorescent staining	1078:1103	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	9	7	theme	mRNA	1642:1645	arg1	expression					1659:1668	the mRNA and protein expression	1638:1668	the mRNA and protein expression of iNOS and COX-2	1638:1686	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	4	8	theme	H.	609:610	arg1	composition					640:650	H. flavum flower EO's chemical composition	609:650	H. flavum flower EO's chemical composition	609:650	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	6	9	theme	in	832:833	arg1	test					841:844	in vitro test	832:844	in vitro test	832:844	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	9	10	theme	protein	1651:1657	arg1	expression					1659:1668	the mRNA and protein expression	1638:1668	the mRNA and protein expression of iNOS and COX-2	1638:1686	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	4	11	theme	flower	619:624	arg1	composition					640:650	H. flavum flower EO's chemical composition	609:650	H. flavum flower EO's chemical composition	609:650	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	13	12	theme	anti-inflammatory	2224:2240	arg1	activity					2242:2249	significant anti-inflammatory activity	2212:2249	significant anti-inflammatory activity	2212:2249	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	3	13	theme	chronic	533:539	arg1	gastritis					541:549	chronic gastritis	533:549	chronic gastritis in China	533:558	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	13	14	theme	NF-κB	2342:2346	arg1	activation					2348:2357	MAPK and NF-κB activation	2333:2357	MAPK and NF-κB activation	2333:2357	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	6	15	theme	LPS-induced	918:928	arg1	release					930:936	the LPS-induced release	914:936	the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages	914:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	6	16	theme	western	1060:1066	arg1	blot					1068:1071	western blot	1060:1071	western blot	1060:1071	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	11	17	theme	LPS-stimulated	2003:2016	arg1	RAW264.7 cells					2018:2031	LPS-stimulated RAW264.7 cells	2003:2031	LPS-stimulated RAW264.7 cells	2003:2031	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	8	18	theme	main	1327:1330	arg1	components					1332:1341	The main components	1323:1341	RESULTS The main components of EO	1315:1347	RESULTS The main components of EO were β-pinene (20.2%), α-pinene (9.3%), α-phellandrene (8.3%), 1,8-cineole (7.1%), E-nerolidol (5.4%), limonene (4.4%), borneol (4.1%), and β-caryophyllene (3.7%).
36280015	6	19	theme	RAW264.7	977:984	arg1	macrophages					986:996	RAW264.7 macrophages	977:996	RAW264.7 macrophages	977:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	10	20	theme	IL-6	1724:1727	arg1	production					1747:1756	IL-6, TNF-α, and IL-1β production	1724:1756	IL-6, TNF-α, and IL-1β production	1724:1756	Meanwhile, it remarkably decreased IL-6, TNF-α, and IL-1β production by inhibiting their mRNA levels.
36280015	13	21	theme	flavum	2194:2199	arg1	EO					2201:2202	H. flavum EO	2191:2202	H. flavum EO	2191:2202	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	1	22	theme	medicinal	211:219	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE	135:164	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum	135:181	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	22	theme	medicinal	211:219	arg1	plant					221:225	an ornamental, edible, and medicinal plant	184:225	an ornamental, edible, and medicinal plant	184:225	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	8	23	dep	RESULTS	1315:1321	arg1	components					1332:1341	The main components	1323:1341	RESULTS The main components of EO	1315:1347	RESULTS The main components of EO were β-pinene (20.2%), α-pinene (9.3%), α-phellandrene (8.3%), 1,8-cineole (7.1%), E-nerolidol (5.4%), limonene (4.4%), borneol (4.1%), and β-caryophyllene (3.7%).
36280015	6	24	theme	EO	881:882	arg1	activity					869:876	the anti-inflammatory activity	847:876	the anti-inflammatory activity of EO	847:882	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	11	25	theme	Related	1791:1797	arg1	studies					1809:1815	Related mechanism studies	1791:1815	Related mechanism studies	1791:1815	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	14	26	theme	high	2457:2460	arg1	potential					2462:2470	high potential	2457:2470	high potential for utilization in the pharmaceutical field	2457:2514	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	7	27	theme	IL-6	1257:1260	arg1	levels					1273:1278	TNF-α, IL-6, and IL-1β levels	1250:1278	TNF-α, IL-6, and IL-1β levels in serum and tissue	1250:1298	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	4	28	theme	anti-inflammatory	668:684	arg1	activities					686:695	its anti-inflammatory activities	664:695	its anti-inflammatory activities	664:695	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	12	29	theme	Further	2034:2040	arg1	assay					2050:2054	Further in vivo assay	2034:2054	Further in vivo assay	2034:2054	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	7	30	theme	TNF-α	1250:1254	arg1	levels					1273:1278	TNF-α, IL-6, and IL-1β levels	1250:1278	TNF-α, IL-6, and IL-1β levels in serum and tissue	1250:1298	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	14	31	dep	H.	2366:2367	arg1	flavum					2369:2374	H. flavum	2366:2374	H. flavum EO	2366:2377	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	10	32	theme	TNF-α	1730:1734	arg1	production					1747:1756	IL-6, TNF-α, and IL-1β production	1724:1756	IL-6, TNF-α, and IL-1β production	1724:1756	Meanwhile, it remarkably decreased IL-6, TNF-α, and IL-1β production by inhibiting their mRNA levels.
36280015	7	33	theme	anti-inflammatory	1134:1150	arg1	potential					1152:1160	EO's in vivo anti-inflammatory potential	1121:1160	EO's in vivo anti-inflammatory potential	1121:1160	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	5	34	theme	chemical	769:776	arg1	composition					778:788	MATERIALS AND METHODS EO's chemical composition	742:788	MATERIALS AND METHODS EO's chemical composition	742:788	MATERIALS AND METHODS EO's chemical composition was determined by GC-FID/MS analysis.
36280015	13	35	theme	action	2294:2299	arg1	mechanism					2281:2289	its mechanism	2277:2289	its mechanism of action	2277:2299	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	11	36	theme	transfer	1929:1936	arg1	blockade					1903:1910	blockade	1903:1910	blockade of NF-κB nuclear transfer	1903:1936	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	6	37	from	IL-1β	951:955	arg1	macrophages					986:996	RAW264.7 macrophages	977:996	RAW264.7 macrophages	977:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	14	38	contain	possess	2449:2455	arg2	potential					2462:2470	high potential	2457:2470	high potential for utilization in the pharmaceutical field	2457:2514	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	14	38	contain	possess	2449:2455	arg1	EO					2376:2377	H. flavum EO	2366:2377	H. flavum EO	2366:2377	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	4	39	dep	in	720:721	arg1	vitro					723:727	vitro	723:727	vitro	723:727	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	6	40	from	TNF-α	958:962	arg1	macrophages					986:996	RAW264.7 macrophages	977:996	RAW264.7 macrophages	977:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	0	41	theme	related	94:100	arg1	mechanisms					102:111	related mechanisms	94:111	related mechanisms	94:111	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	41	theme	related	94:100	arg1	oil					34:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	11	42	theme	MAPKs	1958:1962	arg1	phosphorylation					1984:1998	MAPKs (ERK, p38, and JNK) phosphorylation	1958:1998	MAPKs (ERK, p38, and JNK) phosphorylation	1958:1998	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	6	43	from	NO	941:942	arg1	macrophages					986:996	RAW264.7 macrophages	977:996	RAW264.7 macrophages	977:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	4	44	theme	in	733:734	arg1	activities					686:695	its anti-inflammatory activities	664:695	its anti-inflammatory activities	664:695	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	14	45	theme	promising	2411:2419	arg1	agent					2439:2443	a novel and promising anti-inflammatory agent	2399:2443	a novel and promising anti-inflammatory agent	2399:2443	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	9	46	theme	anti-inflammatory	1521:1537	arg1	activities					1539:1548	the anti-inflammatory activities	1517:1548	the anti-inflammatory activities in vitro	1517:1557	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	6	47	from	PGE2	945:948	arg1	macrophages					986:996	RAW264.7 macrophages	977:996	RAW264.7 macrophages	977:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	1	48	dep	Hedychium	166:174	arg1	flavum					176:181	flavum	176:181	flavum	176:181	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	2	49	theme	inflammation-related	357:376	arg1	diseases					378:385	inflammation-related diseases	357:385	inflammation-related diseases like indigestion, diarrhea, and stomach pain	357:430	Its flower is a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain.
36280015	0	50	theme	Hedychium	0:8	arg1	activities					79:88	anti-inflammatory activities	61:88	anti-inflammatory activities	61:88	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	50	theme	Hedychium	0:8	arg1	mechanisms					102:111	related mechanisms	94:111	related mechanisms	94:111	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	50	theme	Hedychium	0:8	arg1	oil					34:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	7	51	theme	ear	1233:1235	arg1	swelling					1237:1244	ear swelling	1233:1244	ear swelling	1233:1244	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	9	52	theme	in	1550:1551	arg1	activities					1539:1548	the anti-inflammatory activities	1517:1548	the anti-inflammatory activities in vitro	1517:1557	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	0	53	theme	flower	17:22	arg1	activities					79:88	anti-inflammatory activities	61:88	anti-inflammatory activities	61:88	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	53	theme	flower	17:22	arg1	mechanisms					102:111	related mechanisms	94:111	related mechanisms	94:111	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	53	theme	flower	17:22	arg1	oil					34:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	1	54	theme	essential	278:286	arg1	EO					294:295	EO	294:295	EO	294:295	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	54	theme	essential	278:286	arg1	oils					288:291	aromatic essential oils	269:291	aromatic essential oils (EO)	269:296	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	7	55	from	levels	1273:1278	arg1	tissue					1293:1298	tissue	1293:1298	tissue	1293:1298	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	7	55	from	levels	1273:1278	arg1	serum					1283:1287	serum	1283:1287	serum	1283:1287	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	6	56	theme	IL-6	969:972	arg1	release					930:936	the LPS-induced release	914:936	the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages	914:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	11	57	theme	NF-κB	1915:1919	arg1	transfer					1929:1936	NF-κB nuclear transfer	1915:1936	NF-κB nuclear transfer	1915:1936	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	12	58	theme	IL-1β	2145:2149	arg1	levels					2151:2156	TNF-α, IL-6, and IL-1β levels	2128:2156	TNF-α, IL-6, and IL-1β levels in serum and tissue	2128:2176	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	6	59	from	release	930:936	arg1	macrophages					986:996	RAW264.7 macrophages	977:996	RAW264.7 macrophages	977:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	4	60	dep	aimed	592:596	arg1	AIM					561:563	AIM	561:563	AIM OF THE STUDY	561:576	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	11	61	dep	MAPKs	1958:1962	arg1	p38					1970:1972	p38	1970:1972	p38	1970:1972	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	11	61	dep	MAPKs	1958:1962	arg1	JNK					1979:1981	JNK	1979:1981	JNK	1979:1981	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	11	61	dep	MAPKs	1958:1962	arg1	ERK					1965:1967	ERK	1965:1967	ERK	1965:1967	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	11	61	dep	MAPKs	1958:1962	arg1	MAPKs					1958:1962	MAPKs	1958:1962	MAPKs (ERK, p38, and JNK) phosphorylation	1958:1998	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	0	62	theme	Chemical	39:46	arg1	composition					48:58	Chemical composition	39:58	Chemical composition	39:58	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	63	dep	oil	34:36	arg1	composition					48:58	Chemical composition	39:58	Chemical composition	39:58	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	7	64	theme	ear	1207:1209	arg1	model					1217:1221	a xylene-induced ear edema model	1190:1221	a xylene-induced ear edema model	1190:1221	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	3	65	used	used	477:480	arg2	stomachic					514:522	an aromatic stomachic	502:522	an aromatic stomachic	502:522	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	3	65	used	used	477:480	arg2	EO					465:466	H. flavum flower EO	448:466	H. flavum flower EO	448:466	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	14	66	from	utilization	2476:2486	arg1	field					2510:2514	the pharmaceutical field	2491:2514	the pharmaceutical field	2491:2514	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	3	67	theme	flavum	451:456	arg1	EO					465:466	H. flavum flower EO	448:466	H. flavum flower EO	448:466	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	3	67	theme	flavum	451:456	arg1	stomachic					514:522	an aromatic stomachic	502:522	an aromatic stomachic	502:522	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	12	68	dep	in	2042:2043	arg1	vivo					2045:2048	vivo	2045:2048	vivo	2045:2048	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	10	69	theme	mRNA	1778:1781	arg1	levels					1783:1788	their mRNA levels	1772:1788	their mRNA levels	1772:1788	Meanwhile, it remarkably decreased IL-6, TNF-α, and IL-1β production by inhibiting their mRNA levels.
36280015	6	70	dep	in	832:833	arg1	vitro					835:839	vitro	835:839	vitro	835:839	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	7	71	from	swelling	1237:1244	arg1	tissue					1293:1298	tissue	1293:1298	tissue	1293:1298	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	7	71	from	swelling	1237:1244	arg1	serum					1283:1287	serum	1283:1287	serum	1283:1287	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	6	72	theme	related	1012:1018	arg1	mechanisms					1020:1029	its related mechanisms	1008:1029	its related mechanisms	1008:1029	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	9	73	theme	PGE2	1610:1613	arg1	release					1615:1621	the LPS-stimulated NO and PGE2 release	1584:1621	release	1615:1621	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	9	74	dep	in	1550:1551	arg1	vitro					1553:1557	vitro	1553:1557	vitro	1553:1557	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	2	75	theme	Chinese	327:333	arg1	medicine					335:342	a traditional Chinese medicine	313:342	a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain	313:430	Its flower is a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain.
36280015	2	75	theme	Chinese	327:333	arg1	flower					303:308	Its flower	299:308	Its flower	299:308	Its flower is a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain.
36280015	6	76	theme	anti-inflammatory	851:867	arg1	activity					869:876	the anti-inflammatory activity	847:876	the anti-inflammatory activity of EO	847:882	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	6	77	theme	staining	1096:1103	arg1	analysis					1105:1112	qRT-PCR, western blot, and immunofluorescent staining analysis	1051:1112	qRT-PCR, western blot, and immunofluorescent staining analysis	1051:1112	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	12	78	theme	xylene-induced	2083:2096	arg1	edema					2102:2106	xylene-induced ear edema	2083:2106	xylene-induced ear edema in mice	2083:2114	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	6	79	theme	qRT-PCR	1051:1057	arg1	analysis					1105:1112	qRT-PCR, western blot, and immunofluorescent staining analysis	1051:1112	qRT-PCR, western blot, and immunofluorescent staining analysis	1051:1112	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	5	80	theme	GC-FID/MS	808:816	arg1	analysis					818:825	GC-FID/MS analysis	808:825	GC-FID/MS analysis	808:825	MATERIALS AND METHODS EO's chemical composition was determined by GC-FID/MS analysis.
36280015	13	81	theme	significant	2212:2222	arg1	activity					2242:2249	significant anti-inflammatory activity	2212:2249	significant anti-inflammatory activity	2212:2249	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	1	82	theme	ornamental	187:196	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE	135:164	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum	135:181	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	82	theme	ornamental	187:196	arg1	plant					221:225	an ornamental, edible, and medicinal plant	184:225	an ornamental, edible, and medicinal plant	184:225	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	11	83	theme	IκBα	1854:1857	arg1	phosphorylation					1859:1873	IκBα phosphorylation	1854:1873	IκBα phosphorylation	1854:1873	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	6	84	theme	blot	1068:1071	arg1	analysis					1105:1112	qRT-PCR, western blot, and immunofluorescent staining analysis	1051:1112	qRT-PCR, western blot, and immunofluorescent staining analysis	1051:1112	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	4	85	theme	flavum	612:617	arg1	composition					640:650	H. flavum flower EO's chemical composition	609:650	H. flavum flower EO's chemical composition	609:650	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	12	86	from	levels	2151:2156	arg1	tissue					2171:2176	tissue	2171:2176	tissue	2171:2176	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	12	86	from	levels	2151:2156	arg1	serum					2161:2165	serum	2161:2165	serum	2161:2165	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	6	87	from	IL-6	969:972	arg1	macrophages					986:996	RAW264.7 macrophages	977:996	RAW264.7 macrophages	977:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	13	88	theme	activation	2348:2357	arg1	inhibition					2319:2328	the inhibition	2315:2328	the inhibition of MAPK and NF-κB activation	2315:2357	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	1	89	theme	edible	199:204	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE	135:164	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum	135:181	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	89	theme	edible	199:204	arg1	plant					221:225	an ornamental, edible, and medicinal plant	184:225	an ornamental, edible, and medicinal plant	184:225	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	9	90	theme	iNOS	1673:1676	arg1	expression					1659:1668	the mRNA and protein expression	1638:1668	the mRNA and protein expression of iNOS and COX-2	1638:1686	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	1	91	theme	ETHNOPHARMACOLOGICAL	135:154	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE	135:164	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum	135:181	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	91	theme	ETHNOPHARMACOLOGICAL	135:154	arg1	plant					221:225	an ornamental, edible, and medicinal plant	184:225	an ornamental, edible, and medicinal plant	184:225	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	91	theme	ETHNOPHARMACOLOGICAL	135:154	arg1	source					259:264	a source	257:264	a source of aromatic essential oils (EO)	257:296	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	13	92	theme	H.	2191:2192	arg1	EO					2201:2202	H. flavum EO	2191:2202	H. flavum EO	2191:2202	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	8	93	theme	EO	1346:1347	arg1	RESULTS					1315:1321	RESULTS The main components	1315:1341	RESULTS The main components of EO	1315:1347	RESULTS The main components of EO were β-pinene (20.2%), α-pinene (9.3%), α-phellandrene (8.3%), 1,8-cineole (7.1%), E-nerolidol (5.4%), limonene (4.4%), borneol (4.1%), and β-caryophyllene (3.7%).
36280015	9	94	theme	COX-2	1682:1686	arg1	expression					1659:1668	the mRNA and protein expression	1638:1668	the mRNA and protein expression of iNOS and COX-2	1638:1686	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	7	95	theme	IL-1β	1267:1271	arg1	levels					1273:1278	TNF-α, IL-6, and IL-1β levels	1250:1278	TNF-α, IL-6, and IL-1β levels in serum and tissue	1250:1298	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	14	96	theme	pharmaceutical	2495:2508	arg1	field					2510:2514	the pharmaceutical field	2491:2514	the pharmaceutical field	2491:2514	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	6	97	theme	TNF-α	958:962	arg1	release					930:936	the LPS-induced release	914:936	the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages	914:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	11	98	theme	mechanism	1799:1807	arg1	studies					1809:1815	Related mechanism studies	1791:1815	Related mechanism studies	1791:1815	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	12	99	theme	in	2042:2043	arg1	assay					2050:2054	Further in vivo assay	2034:2054	Further in vivo assay	2034:2054	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	0	100	theme	anti-inflammatory	61:77	arg1	activities					79:88	anti-inflammatory activities	61:88	anti-inflammatory activities	61:88	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	100	theme	anti-inflammatory	61:77	arg1	oil					34:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	4	101	dep	in	733:734	arg1	vivo					736:739	vivo	736:739	vivo	736:739	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	6	102	theme	IL-1β	951:955	arg1	release					930:936	the LPS-induced release	914:936	the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages	914:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	7	103	theme	EO	1121:1122	arg1	potential					1152:1160	EO's in vivo anti-inflammatory potential	1121:1160	EO's in vivo anti-inflammatory potential	1121:1160	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	13	104	theme	MAPK	2333:2336	arg1	activation					2348:2357	MAPK and NF-κB activation	2333:2357	MAPK and NF-κB activation	2333:2357	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	1	105	theme	Hedychium	166:174	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE	135:164	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum	135:181	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	105	theme	Hedychium	166:174	arg1	plant					221:225	an ornamental, edible, and medicinal plant	184:225	an ornamental, edible, and medicinal plant	184:225	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	105	theme	Hedychium	166:174	arg1	source					259:264	a source	257:264	a source of aromatic essential oils (EO)	257:296	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	6	106	theme	PGE2	945:948	arg1	release					930:936	the LPS-induced release	914:936	the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages	914:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	13	107	dep	CONCLUSIONS	2179:2189	arg1	exerted					2204:2210	exerted	2204:2210	exerted significant anti-inflammatory activity in vivo and in vitro	2204:2270	CONCLUSIONS H. flavum EO exerted significant anti-inflammatory activity in vivo and in vitro, and its mechanism of action is related to the inhibition of MAPK and NF-κB activation.
36280015	4	108	theme	chemical	631:638	arg1	composition					640:650	H. flavum flower EO's chemical composition	609:650	H. flavum flower EO's chemical composition	609:650	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	6	109	theme	NO	941:942	arg1	release					930:936	the LPS-induced release	914:936	the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages	914:996	For in vitro test, the anti-inflammatory activity of EO was demonstrated by measuring the LPS-induced release of NO, PGE2, IL-1β, TNF-α, and IL-6 in RAW264.7 macrophages, and then its related mechanisms were explored using qRT-PCR, western blot, and immunofluorescent staining analysis.
36280015	4	110	theme	in	720:721	arg1	activities					686:695	its anti-inflammatory activities	664:695	its anti-inflammatory activities	664:695	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	10	111	theme	IL-1β	1741:1745	arg1	production					1747:1756	IL-6, TNF-α, and IL-1β production	1724:1756	IL-6, TNF-α, and IL-1β production	1724:1756	Meanwhile, it remarkably decreased IL-6, TNF-α, and IL-1β production by inhibiting their mRNA levels.
36280015	14	112	theme	anti-inflammatory	2421:2437	arg1	agent					2439:2443	a novel and promising anti-inflammatory agent	2399:2443	a novel and promising anti-inflammatory agent	2399:2443	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	0	113	theme	flavum	10:15	arg1	activities					79:88	anti-inflammatory activities	61:88	anti-inflammatory activities	61:88	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	113	theme	flavum	10:15	arg1	mechanisms					102:111	related mechanisms	94:111	related mechanisms	94:111	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	113	theme	flavum	10:15	arg1	oil					34:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	3	114	from	gastritis	541:549	arg1	China					554:558	China	554:558	China	554:558	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	0	115	theme	essential	24:32	arg1	activities					79:88	anti-inflammatory activities	61:88	anti-inflammatory activities	61:88	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	115	theme	essential	24:32	arg1	mechanisms					102:111	related mechanisms	94:111	related mechanisms	94:111	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	0	115	theme	essential	24:32	arg1	oil					34:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil	0:36	Hedychium flavum flower essential oil: Chemical composition, anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	1	116	theme	aromatic	269:276	arg1	EO					294:295	EO	294:295	EO	294:295	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	116	theme	aromatic	269:276	arg1	oils					288:291	aromatic essential oils	269:291	aromatic essential oils (EO)	269:296	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	4	117	theme	related	701:707	arg1	mechanisms					709:718	related mechanisms	701:718	related mechanisms	701:718	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	3	118	theme	H.	448:449	arg1	EO					465:466	H. flavum flower EO	448:466	H. flavum flower EO	448:466	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	3	118	theme	H.	448:449	arg1	stomachic					514:522	an aromatic stomachic	502:522	an aromatic stomachic	502:522	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	1	119	theme	oils	288:291	arg1	RELEVANCE					156:164	ETHNOPHARMACOLOGICAL RELEVANCE	135:164	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum	135:181	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	1	119	theme	oils	288:291	arg1	source					259:264	a source	257:264	a source of aromatic essential oils (EO)	257:296	ETHNOPHARMACOLOGICAL RELEVANCE Hedychium flavum, an ornamental, edible, and medicinal plant, is extensively cultivated as a source of aromatic essential oils (EO).
36280015	7	120	theme	edema	1211:1215	arg1	model					1217:1221	a xylene-induced ear edema model	1190:1221	a xylene-induced ear edema model	1190:1221	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	11	121	theme	nuclear	1921:1927	arg1	transfer					1929:1936	NF-κB nuclear transfer	1915:1936	NF-κB nuclear transfer	1915:1936	Related mechanism studies indicated that it not only inhibited IκBα phosphorylation and degradation, leading to blockade of NF-κB nuclear transfer but also suppressed MAPKs (ERK, p38, and JNK) phosphorylation in LPS-stimulated RAW264.7 cells.
36280015	12	122	theme	TNF-α	2128:2132	arg1	levels					2151:2156	TNF-α, IL-6, and IL-1β levels	2128:2156	TNF-α, IL-6, and IL-1β levels in serum and tissue	2128:2176	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	2	123	dep	indigestion	392:402	arg1	pain					427:430	pain	427:430	pain	427:430	Its flower is a traditional Chinese medicine for treating inflammation-related diseases like indigestion, diarrhea, and stomach pain.
36280015	5	124	theme	MATERIALS	742:750	arg1	EO					764:765	MATERIALS AND METHODS EO	742:765	MATERIALS AND METHODS EO's chemical composition	742:788	MATERIALS AND METHODS EO's chemical composition was determined by GC-FID/MS analysis.
36280015	7	125	theme	xylene-induced	1192:1205	arg1	model					1217:1221	a xylene-induced ear edema model	1190:1221	a xylene-induced ear edema model	1190:1221	Next, EO's in vivo anti-inflammatory potential was further evaluated using a xylene-induced ear edema model, in which ear swelling and TNF-α, IL-6, and IL-1β levels in serum and tissue were examined.
36280015	12	126	theme	IL-6	2135:2138	arg1	levels					2151:2156	TNF-α, IL-6, and IL-1β levels	2128:2156	TNF-α, IL-6, and IL-1β levels in serum and tissue	2128:2176	Further in vivo assay showed that EO ameliorated xylene-induced ear edema in mice and reduced TNF-α, IL-6, and IL-1β levels in serum and tissue.
36280015	14	127	theme	H.	2366:2367	arg1	EO					2376:2377	H. flavum EO	2366:2377	H. flavum EO	2366:2377	Thus, H. flavum EO could be considered a novel and promising anti-inflammatory agent and possess high potential for utilization in the pharmaceutical field.
36280015	9	128	theme	LPS-stimulated	1588:1601	arg1	NO					1603:1604	the LPS-stimulated NO and PGE2 release	1584:1621	NO	1603:1604	For the anti-inflammatory activities in vitro, EO dramatically reduced the LPS-stimulated NO and PGE2 release by suppressing the mRNA and protein expression of iNOS and COX-2.
36280015	3	129	theme	flower	458:463	arg1	EO					465:466	H. flavum flower EO	448:466	H. flavum flower EO	448:466	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	3	129	theme	flower	458:463	arg1	stomachic					514:522	an aromatic stomachic	502:522	an aromatic stomachic	502:522	In particular, H. flavum flower EO has been used in cosmetics and as an aromatic stomachic to treat chronic gastritis in China.
36280015	4	130	theme	STUDY	572:576	arg1	AIM					561:563	AIM	561:563	AIM OF THE STUDY	561:576	AIM OF THE STUDY This research aimed to analyze H. flavum flower EO's chemical composition and explore its anti-inflammatory activities and related mechanisms in vitro and in vivo.
36280015	5	131	theme	METHODS	756:762	arg1	EO					764:765	MATERIALS AND METHODS EO	742:765	MATERIALS AND METHODS EO's chemical composition	742:788	MATERIALS AND METHODS EO's chemical composition was determined by GC-FID/MS analysis.
36234818	2	0	theme	work	340:343	arg1	aims					320:323	the aims	316:323	the aims of the present work	316:343	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	4	1	theme	chlorogenic	755:765	arg1	acid					782:785	gentisic acid	773:785	gentisic acid	773:785	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	1	theme	chlorogenic	755:765	arg1	3-hydroxytyrosol					792:807	3-hydroxytyrosol	792:807	3-hydroxytyrosol	792:807	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	1	theme	chlorogenic	755:765	arg1	phenolics					826:834	the prominent phenolics	812:834	the prominent phenolics	812:834	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	1	theme	chlorogenic	755:765	arg1	catechin					745:752	catechin	745:752	catechin	745:752	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	1	theme	chlorogenic	755:765	arg1	acid					767:770	chlorogenic acid	755:770	chlorogenic acid	755:770	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	6	2	theme	pro-inflammatory	976:991	arg1	IL-6					993:996	pro-inflammatory IL-6	976:996	pro-inflammatory IL-6	976:996	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	0	3	from	Effects	11:17	arg1	Prostates					91:99	Isolated Prostates	82:99	Isolated Prostates	82:99	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	0	3	from	Effects	11:17	arg1	Ovaries					105:111	Ovaries	105:111	Ovaries	105:111	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	2	4	theme	OVCAR-3	558:564	arg1	cells					566:570	human ovary cancer OVCAR-3 cells	539:570	human ovary cancer OVCAR-3 cells	539:570	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	5	5	theme	cytotoxic	874:882	arg1	effect					884:889	a relevant cytotoxic effect	863:889	a relevant cytotoxic effect	863:889	The extract did not exert a relevant cytotoxic effect on PC3 and OVCAR-3 cells.
36234818	2	6	theme	present	332:338	arg1	work					340:343	the present work	328:343	the present work	328:343	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	8	7	theme	formulation	1491:1501	arg1	effects					1475:1481	anti-inflammatory and antioxidant effects	1441:1481	anti-inflammatory and antioxidant effects of this formulation	1441:1501	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	1	8	theme	pelvic	264:269	arg1	PID					293:295	PID	293:295	PID	293:295	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	1	8	theme	pelvic	264:269	arg1	disease					284:290	pelvic inflammatory disease	264:290	pelvic inflammatory disease (PID)	264:296	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	7	9	theme	TRPV1	1334:1338	arg1	receptor					1340:1347	the TRPV1 receptor	1330:1347	the TRPV1 receptor	1330:1347	Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation.
36234818	2	10	theme	cancer	551:556	arg1	cells					566:570	human ovary cancer OVCAR-3 cells	539:570	human ovary cancer OVCAR-3 cells	539:570	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	6	11	theme	SOD	1141:1143	arg1	expression					1150:1159	CAT and SOD gene expression	1133:1159	expression	1150:1159	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	7	12	theme	catechin	1283:1290	arg1	capability					1269:1278	the capability	1265:1278	the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation	1265:1395	Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation.
36234818	1	13	theme	inflammatory	271:282	arg1	PID					293:295	PID	293:295	PID	293:295	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	1	13	theme	inflammatory	271:282	arg1	disease					284:290	pelvic inflammatory disease	264:290	pelvic inflammatory disease (PID)	264:296	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	0	14	theme	Isolated	82:89	arg1	Prostates					91:99	Isolated Prostates	82:99	Isolated Prostates	82:99	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	2	15	theme	ovary	545:549	arg1	cells					566:570	human ovary cancer OVCAR-3 cells	539:570	human ovary cancer OVCAR-3 cells	539:570	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	8	16	from	capability	1525:1534	arg1	management					1543:1552	the management	1539:1552	the management of the clinical symptoms related to prostatitis and PID	1539:1608	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	4	17	theme	gentisic	773:780	arg1	acid					782:785	gentisic acid	773:785	gentisic acid	773:785	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	17	theme	gentisic	773:780	arg1	3-hydroxytyrosol					792:807	3-hydroxytyrosol	792:807	3-hydroxytyrosol	792:807	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	17	theme	gentisic	773:780	arg1	phenolics					826:834	the prominent phenolics	812:834	the prominent phenolics	812:834	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	17	theme	gentisic	773:780	arg1	catechin					745:752	catechin	745:752	catechin	745:752	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	17	theme	gentisic	773:780	arg1	acid					767:770	chlorogenic acid	755:770	chlorogenic acid	755:770	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	6	18	theme	dose-dependent	947:960	arg1	inhibition					962:971	a dose-dependent inhibition	945:971	a dose-dependent inhibition of pro-inflammatory IL-6	945:996	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	2	19	theme	cytotoxic	481:489	arg1	effects					491:497	the extract's cytotoxic effects	467:497	the extract's cytotoxic effects	467:497	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	6	20	from	inhibition	962:971	arg1	specimens					1046:1054	prostate and ovary specimens	1027:1054	specimens	1046:1054	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	6	21	theme	prostate	1027:1034	arg1	specimens					1046:1054	prostate and ovary specimens	1027:1054	specimens	1046:1054	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	6	22	theme	gene	1145:1148	arg1	expression					1150:1159	CAT and SOD gene expression	1133:1159	expression	1150:1159	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	1	23	theme	innovative	173:182	arg1	approach					184:191	an innovative approach	170:191	an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID)	170:296	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	2	24	theme	gel	437:439	arg1	capsules					441:448	PollenAid Plus soft gel capsules	417:448	PollenAid Plus soft gel capsules	417:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	4	25	theme	prominent	816:824	arg1	acid					782:785	gentisic acid	773:785	gentisic acid	773:785	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	25	theme	prominent	816:824	arg1	3-hydroxytyrosol					792:807	3-hydroxytyrosol	792:807	3-hydroxytyrosol	792:807	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	25	theme	prominent	816:824	arg1	phenolics					826:834	the prominent phenolics	812:834	the prominent phenolics	812:834	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	25	theme	prominent	816:824	arg1	catechin					745:752	catechin	745:752	catechin	745:752	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	4	25	theme	prominent	816:824	arg1	acid					767:770	chlorogenic acid	755:770	chlorogenic acid	755:770	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	2	26	theme	Plus	427:430	arg1	capsules					441:448	PollenAid Plus soft gel capsules	417:448	PollenAid Plus soft gel capsules	417:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	2	27	theme	phenolic	365:372	arg1	composition					374:384	the phenolic composition	361:384	the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules	361:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	2	28	theme	soft	432:435	arg1	capsules					441:448	PollenAid Plus soft gel capsules	417:448	PollenAid Plus soft gel capsules	417:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	8	29	theme	symptoms	1570:1577	arg1	management					1543:1552	the management	1539:1552	the management of the clinical symptoms related to prostatitis and PID	1539:1608	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	6	30	from	expression	1013:1022	arg1	specimens					1046:1054	prostate and ovary specimens	1027:1054	specimens	1046:1054	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	4	31	theme	phytochemical	706:718	arg1	investigation					720:732	The phytochemical investigation	702:732	The phytochemical investigation	702:732	The phytochemical investigation identified catechin, chlorogenic acid, gentisic acid, and 3-hydroxytyrosol as the prominent phenolics.
36234818	7	32	theme	docking	1238:1244	arg1	approach					1246:1253	a docking approach	1236:1253	a docking approach	1236:1253	Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation.
36234818	0	33	theme	Protective	0:9	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.	0:142	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	3	34	theme	prostate	636:643	arg1	specimens					655:663	isolated prostate and ovary specimens	627:663	isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS)	627:699	Additionally, protective effects were investigated in isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS).
36234818	6	35	theme	antioxidant	1198:1208	arg1	systems					1218:1224	tissue antioxidant defense systems	1191:1224	tissue antioxidant defense systems	1191:1224	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	6	36	theme	CAT	1133:1135	arg1	expression					1150:1159	CAT and SOD gene expression	1133:1159	expression	1150:1159	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	6	37	theme	ovary	1040:1044	arg1	specimens					1046:1054	prostate and ovary specimens	1027:1054	specimens	1046:1054	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	0	38	theme	PollenAid	22:30	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.	0:142	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	2	39	theme	cancer	518:523	arg1	cells					529:533	human prostate cancer PC3 cells	503:533	human prostate cancer PC3 cells	503:533	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	7	40	theme	master	1360:1365	arg1	role					1367:1370	a master role	1358:1370	a master role	1358:1370	Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation.
36234818	2	41	theme	extract	406:412	arg1	composition					374:384	the phenolic composition	361:384	the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules	361:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	7	42	theme	prostate	1375:1382	arg1	inflammation					1384:1395	prostate inflammation	1375:1395	prostate inflammation	1375:1395	Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation.
36234818	2	43	theme	prostate	509:516	arg1	cells					529:533	human prostate cancer PC3 cells	503:533	human prostate cancer PC3 cells	503:533	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	8	44	theme	present	1411:1417	arg1	findings					1419:1426	the present findings	1407:1426	the present findings	1407:1426	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	2	45	theme	hydroalcoholic	391:404	arg1	extract					406:412	a hydroalcoholic extract	389:412	a hydroalcoholic extract of PollenAid Plus soft gel capsules	389:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	0	46	theme	Soft	37:40	arg1	Capsules					46:53	Soft Gel Capsules	37:53	Soft Gel Capsules	37:53	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	2	47	theme	human	503:507	arg1	cells					529:533	human prostate cancer PC3 cells	503:533	human prostate cancer PC3 cells	503:533	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	8	48	theme	antioxidant	1463:1473	arg1	effects					1475:1481	anti-inflammatory and antioxidant effects	1441:1481	anti-inflammatory and antioxidant effects of this formulation	1441:1501	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	6	49	theme	defense	1210:1216	arg1	systems					1218:1224	tissue antioxidant defense systems	1191:1224	tissue antioxidant defense systems	1191:1224	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	7	50	theme	chlorogenic	1296:1306	arg1	acid					1308:1311	chlorogenic acid	1296:1311	chlorogenic acid	1296:1311	Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation.
36234818	6	51	theme	gene	1008:1011	arg1	expression					1013:1022	TNF-α gene expression	1002:1022	TNF-α gene expression in prostate and ovary specimens	1002:1054	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	7	52	theme	acid	1308:1311	arg1	capability					1269:1278	the capability	1265:1278	the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation	1265:1395	Finally, a docking approach suggested the capability of catechin and chlorogenic acid to interact with the TRPV1 receptor, playing a master role in prostate inflammation.
36234818	2	53	theme	PollenAid	417:425	arg1	capsules					441:448	PollenAid Plus soft gel capsules	417:448	PollenAid Plus soft gel capsules	417:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	1	54	theme	clinical	219:226	arg1	symptoms					228:235	the clinical symptoms	215:235	the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID)	215:296	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	0	55	theme	Capsules	46:53	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.	0:142	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	2	56	theme	human	539:543	arg1	cells					566:570	human ovary cancer OVCAR-3 cells	539:570	human ovary cancer OVCAR-3 cells	539:570	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	6	57	theme	tissue	1191:1196	arg1	systems					1218:1224	tissue antioxidant defense systems	1191:1224	tissue antioxidant defense systems	1191:1224	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	6	58	theme	LPS-induced	1105:1115	arg1	upregulation					1117:1128	the LPS-induced upregulation	1101:1128	the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems	1101:1224	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	0	59	theme	Gel	42:44	arg1	Capsules					46:53	Soft Gel Capsules	37:53	Soft Gel Capsules	37:53	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	6	60	theme	expression	1150:1159	arg1	upregulation					1117:1128	the LPS-induced upregulation	1101:1128	the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems	1101:1224	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	8	61	theme	anti-inflammatory	1441:1457	arg1	effects					1475:1481	anti-inflammatory and antioxidant effects	1441:1481	anti-inflammatory and antioxidant effects of this formulation	1441:1501	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	8	62	theme	clinical	1561:1568	arg1	symptoms					1570:1577	the clinical symptoms	1557:1577	the clinical symptoms related to prostatitis and PID	1557:1608	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	3	63	theme	ovary	649:653	arg1	specimens					655:663	isolated prostate and ovary specimens	627:663	isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS)	627:699	Additionally, protective effects were investigated in isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS).
36234818	0	64	dep	PollenAid	22:30	arg1	Extract					71:77	' Hydroalcoholic Extract	54:77	' Hydroalcoholic Extract	54:77	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	6	65	theme	TNF-α	1002:1006	arg1	expression					1013:1022	TNF-α gene expression	1002:1022	TNF-α gene expression in prostate and ovary specimens	1002:1054	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	1	66	theme	symptoms	228:235	arg1	management					201:210	the management	197:210	the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID)	197:296	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	2	67	theme	PC3	525:527	arg1	cells					529:533	human prostate cancer PC3 cells	503:533	human prostate cancer PC3 cells	503:533	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	3	68	theme	isolated	627:634	arg1	specimens					655:663	isolated prostate and ovary specimens	627:663	isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS)	627:699	Additionally, protective effects were investigated in isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS).
36234818	3	69	theme	protective	587:596	arg1	effects					598:604	protective effects	587:604	protective effects	587:604	Additionally, protective effects were investigated in isolated prostate and ovary specimens exposed to lipopolysaccharide (LPS).
36234818	5	70	theme	OVCAR-3	902:908	arg1	cells					910:914	OVCAR-3 cells	902:914	OVCAR-3 cells	902:914	The extract did not exert a relevant cytotoxic effect on PC3 and OVCAR-3 cells.
36234818	1	71	theme	related	237:243	arg1	symptoms					228:235	the clinical symptoms	215:235	the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID)	215:296	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	0	72	theme	Hydroalcoholic	56:69	arg1	Extract					71:77	' Hydroalcoholic Extract	54:77	' Hydroalcoholic Extract	54:77	Protective Effects of PollenAid Plus Soft Gel Capsules' Hydroalcoholic Extract in Isolated Prostates and Ovaries Exposed to Lipopolysaccharide.
36234818	2	73	from	effects	491:497	arg1	cells					529:533	human prostate cancer PC3 cells	503:533	human prostate cancer PC3 cells	503:533	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	2	73	from	effects	491:497	arg1	cells					566:570	human ovary cancer OVCAR-3 cells	539:570	human ovary cancer OVCAR-3 cells	539:570	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	1	74	theme	Pollen	144:149	arg1	extract					151:157	Pollen extract	144:157	Pollen extract	144:157	Pollen extract represents an innovative approach for the management of the clinical symptoms related to prostatitis and pelvic inflammatory disease (PID).
36234818	2	75	theme	capsules	441:448	arg1	extract					406:412	a hydroalcoholic extract	389:412	a hydroalcoholic extract of PollenAid Plus soft gel capsules	389:448	In this context, the aims of the present work were to analyze the phenolic composition of a hydroalcoholic extract of PollenAid Plus soft gel capsules, and to evaluate the extract's cytotoxic effects, in human prostate cancer PC3 cells and human ovary cancer OVCAR-3 cells.
36234818	8	76	theme	related	1579:1585	arg1	symptoms					1570:1577	the clinical symptoms	1557:1577	the clinical symptoms related to prostatitis and PID	1557:1608	Overall, the present findings demonstrated anti-inflammatory and antioxidant effects of this formulation; thus, suggesting its capability in the management of the clinical symptoms related to prostatitis and PID.
36234818	5	77	theme	relevant	865:872	arg1	effect					884:889	a relevant cytotoxic effect	863:889	a relevant cytotoxic effect	863:889	The extract did not exert a relevant cytotoxic effect on PC3 and OVCAR-3 cells.
36234818	6	78	theme	IL-6	993:996	arg1	expression					1013:1022	TNF-α gene expression	1002:1022	TNF-α gene expression in prostate and ovary specimens	1002:1054	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36234818	6	78	theme	IL-6	993:996	arg1	inhibition					962:971	a dose-dependent inhibition	945:971	a dose-dependent inhibition of pro-inflammatory IL-6	945:996	However, the extract showed a dose-dependent inhibition of pro-inflammatory IL-6 and TNF-α gene expression in prostate and ovary specimens, and the extract was effective in preventing the LPS-induced upregulation of CAT and SOD gene expression, which are deeply involved in tissue antioxidant defense systems.
36142169	8	0	theme	siRNA	1098:1102	arg1	transfection					1104:1115	siRNA transfection	1098:1115	siRNA transfection	1098:1115	ZNF281 was downregulated by siRNA transfection, and RNA-sequencing was performed to identify genes regulated by TGFβ1 in activated colon fibroblasts via ZNF281.
36142169	6	1	theme	sulfate	888:894	arg1	DSS					904:906	DSS	904:906	DSS	904:906	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	6	1	theme	sulfate	888:894	arg1	sodium					896:901	dextran sulfate sodium	880:901	dextran sulfate sodium (DSS)	880:907	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	10	2	theme	specific	1480:1487	arg1	pathways					1489:1496	specific pathways	1480:1496	specific pathways linked to fibroblast activation and differentiation into myofibroblasts	1480:1568	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	6	3	theme	C57BL/6J	793:800	arg1	mice					802:805	C57BL/6J mice	793:805	C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS)	793:907	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	11	4	theme	activation	1638:1647	arg1	regulator					1608:1616	a key regulator	1602:1616	a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction	1602:1809	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	11	4	theme	activation	1638:1647	arg1	ZNF281					1592:1597	ZNF281	1592:1597	ZNF281	1592:1597	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	5	5	theme	study	658:662	arg1	aim					646:648	The aim	642:648	The aim of this study	642:662	The aim of this study is to investigate in vivo and in vitro the role of ZNF281 in intestinal fibrogenesis.
36142169	8	6	theme	activated	1191:1199	arg1	fibroblasts					1207:1217	activated colon fibroblasts	1191:1217	activated colon fibroblasts	1191:1217	ZNF281 was downregulated by siRNA transfection, and RNA-sequencing was performed to identify genes regulated by TGFβ1 in activated colon fibroblasts via ZNF281.
36142169	10	7	theme	fibroblast	1508:1517	arg1	activation					1519:1528	fibroblast activation	1508:1528	fibroblast activation	1508:1528	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	11	8	theme	colon	1621:1625	arg1	activation					1638:1647	colon fibroblast activation	1621:1647	colon fibroblast activation	1621:1647	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	7	9	theme	pro-fibrotic	1041:1052	arg1	TGFβ1					1063:1067	the pro-fibrotic cytokine TGFβ1	1037:1067	the pro-fibrotic cytokine TGFβ1	1037:1067	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	11	10	theme	cell	1794:1797	arg1	contraction					1799:1809	cell contraction	1794:1809	cell contraction	1794:1809	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	4	11	theme	cancer	634:639	arg1	regulation					592:601	the regulation	588:601	the regulation of pluripotency, stemness, and cancer	588:639	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	2	12	theme	recurrent	275:283	arg1	stenosis					285:292	recurrent stenosis	275:292	recurrent stenosis	275:292	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	1	13	theme	tract	183:187	arg1	colitis					111:117	ulcerative colitis	100:117	ulcerative colitis (UC)	100:122	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	13	theme	tract	183:187	arg1	disorders					149:157	chronic inflammatory disorders	128:157	chronic inflammatory disorders of the gastrointestinal tract	128:187	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	13	theme	tract	183:187	arg1	disease					83:89	Crohn's disease	75:89	Crohn's disease (CD)	75:94	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	4	14	theme	transcriptional	424:438	arg1	regulator					440:448	a zinc-finger transcriptional regulator	410:448	a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer	410:639	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	4	14	theme	transcriptional	424:438	arg1	ZNF281					400:405	ZNF281	400:405	ZNF281	400:405	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	4	14	theme	transcriptional	424:438	arg1	transition					510:519	an epithelial-to-mesenchymal transition	481:519	an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor	481:555	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	7	15	theme	fibroblast	993:1002	arg1	line					1009:1012	the human colon fibroblast cell line	977:1012	the human colon fibroblast cell line CCD-18Co	977:1021	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	1	16	theme	ulcerative	100:109	arg1	colitis					111:117	ulcerative colitis	100:117	ulcerative colitis (UC)	100:122	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	16	theme	ulcerative	100:109	arg1	disease					83:89	Crohn's disease	75:89	Crohn's disease (CD)	75:94	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	16	theme	ulcerative	100:109	arg1	disorders					149:157	chronic inflammatory disorders	128:157	chronic inflammatory disorders of the gastrointestinal tract	128:187	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	16	theme	ulcerative	100:109	arg1	UC					120:121	UC	120:121	UC	120:121	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	7	17	theme	human	981:985	arg1	line					1009:1012	the human colon fibroblast cell line	977:1012	the human colon fibroblast cell line CCD-18Co	977:1021	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	11	18	theme	extracellular	1738:1750	arg1	ECM					1760:1762	ECM	1760:1762	ECM	1760:1762	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	11	18	theme	extracellular	1738:1750	arg1	matrix					1752:1757	extracellular matrix	1738:1757	transcriptionally controlling extracellular matrix (ECM) composition	1708:1775	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	5	19	theme	intestinal	725:734	arg1	fibrogenesis					736:747	intestinal fibrogenesis	725:747	intestinal fibrogenesis	725:747	The aim of this study is to investigate in vivo and in vitro the role of ZNF281 in intestinal fibrogenesis.
36142169	4	20	theme	epithelial-to-mesenchymal	484:508	arg1	regulator					440:448	a zinc-finger transcriptional regulator	410:448	a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer	410:639	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	4	20	theme	epithelial-to-mesenchymal	484:508	arg1	transition					510:519	an epithelial-to-mesenchymal transition	481:519	an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor	481:555	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	4	20	theme	epithelial-to-mesenchymal	484:508	arg1	EMT					522:524	EMT	522:524	EMT	522:524	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	6	21	theme	administration	862:875	arg1	cycles					852:857	two or three cycles	839:857	two or three cycles of administration of dextran sulfate sodium (DSS)	839:907	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	9	22	theme	marked	1248:1253	arg1	increase					1255:1262	a marked increase	1246:1262	a marked increase of ZNF281 in in vivo murine fibrotic colon	1246:1305	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	1	23	theme	chronic	128:134	arg1	colitis					111:117	ulcerative colitis	100:117	ulcerative colitis (UC)	100:122	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	23	theme	chronic	128:134	arg1	disorders					149:157	chronic inflammatory disorders	128:157	chronic inflammatory disorders of the gastrointestinal tract	128:187	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	23	theme	chronic	128:134	arg1	disease					83:89	Crohn's disease	75:89	Crohn's disease (CD)	75:94	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	10	24	theme	TGFβ1-treated	1408:1420	arg1	fibroblasts					1422:1432	TGFβ1-treated fibroblasts	1408:1432	TGFβ1-treated fibroblasts	1408:1432	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	5	25	theme	ZNF281	715:720	arg1	role					707:710	the role	703:710	the role of ZNF281 in intestinal fibrogenesis	703:747	The aim of this study is to investigate in vivo and in vitro the role of ZNF281 in intestinal fibrogenesis.
36142169	11	26	dep	onset	1843:1847	arg1	the					1839:1841	the	1839:1841	the	1839:1841	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	0	27	from	Activation	33:42	arg1	Fibrosis					65:72	TGFβ1-Induced Gut Fibrosis	47:72	TGFβ1-Induced Gut Fibrosis	47:72	ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis.
36142169	10	28	theme	ZNF281	1398:1403	arg1	abrogation					1384:1393	abrogation	1384:1393	abrogation of ZNF281 in TGFβ1-treated fibroblasts	1384:1432	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	11	29	theme	fibrosis	1872:1879	arg1	progression					1853:1863	progression	1853:1863	progression	1853:1863	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	11	29	theme	fibrosis	1872:1879	arg1	onset					1843:1847	onset	1843:1847	onset	1843:1847	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	3	30	theme	molecular	348:356	arg1	unclear					391:397	unclear	391:397	unclear	391:397	Currently, the underlying molecular mechanisms of fibrosis are still unclear.
36142169	3	30	theme	molecular	348:356	arg1	mechanisms					358:367	the underlying molecular mechanisms	333:367	the underlying molecular mechanisms of fibrosis	333:379	Currently, the underlying molecular mechanisms of fibrosis are still unclear.
36142169	0	31	theme	Colon	16:20	arg1	Activation					33:42	Colon Fibroblast Activation	16:42	Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis	16:72	ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis.
36142169	9	32	theme	fibrotic	1292:1299	arg1	colon					1301:1305	in vivo murine fibrotic colon	1277:1305	in vivo murine fibrotic colon	1277:1305	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	6	33	with	mice	802:805	arg1	colitis					820:826	chronic colitis	812:826	chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS)	812:907	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	11	34	theme	fibrotic	1688:1695	arg1	stimuli					1697:1703	fibrotic stimuli	1688:1703	fibrotic stimuli	1688:1703	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	9	35	dep	in	1277:1278	arg1	vivo					1280:1283	vivo	1280:1283	vivo	1280:1283	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	11	36	theme	differentiation	1667:1681	arg1	regulator					1608:1616	a key regulator	1602:1616	a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction	1602:1809	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	11	36	theme	differentiation	1667:1681	arg1	ZNF281					1592:1597	ZNF281	1592:1597	ZNF281	1592:1597	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	0	37	theme	TGFβ1-Induced	47:59	arg1	Fibrosis					65:72	TGFβ1-Induced Gut Fibrosis	47:72	TGFβ1-Induced Gut Fibrosis	47:72	ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis.
36142169	4	38	dep	transition	510:519	arg1	factor					550:555	-inducing transcription factor	526:555	an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor	481:555	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	4	39	theme	transcription	536:548	arg1	factor					550:555	-inducing transcription factor	526:555	an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor	481:555	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	10	40	theme	genes	1461:1465	arg1	expression					1447:1456	the expression	1443:1456	the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts	1443:1568	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	11	41	theme	controlling	1726:1736	arg1	composition					1765:1775	transcriptionally controlling extracellular matrix (ECM) composition	1708:1775	transcriptionally controlling extracellular matrix (ECM) composition	1708:1775	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	2	42	theme	CD	309:310	arg1	patients					312:319	CD patients	309:319	CD patients	309:319	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	9	43	theme	in	1321:1322	arg1	fibroblasts					1342:1352	in vitro human colon fibroblasts	1321:1352	in vitro human colon fibroblasts activated by TGFβ1	1321:1371	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	4	44	theme	pluripotency	606:617	arg1	regulation					592:601	the regulation	588:601	the regulation of pluripotency, stemness, and cancer	588:639	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	9	45	theme	human	1330:1334	arg1	fibroblasts					1342:1352	in vitro human colon fibroblasts	1321:1352	in vitro human colon fibroblasts activated by TGFβ1	1321:1371	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	6	46	theme	sodium	896:901	arg1	administration					862:875	administration	862:875	administration of dextran sulfate sodium (DSS)	862:907	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	4	47	theme	stemness	620:627	arg1	regulation					592:601	the regulation	588:601	the regulation of pluripotency, stemness, and cancer	588:639	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	6	48	theme	dextran	880:886	arg1	DSS					904:906	DSS	904:906	DSS	904:906	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	6	48	theme	dextran	880:886	arg1	sodium					896:901	dextran sulfate sodium	880:901	dextran sulfate sodium (DSS)	880:907	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	11	49	theme	key	1604:1606	arg1	regulator					1608:1616	a key regulator	1602:1616	a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction	1602:1809	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	11	49	theme	key	1604:1606	arg1	ZNF281					1592:1597	ZNF281	1592:1597	ZNF281	1592:1597	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	7	50	theme	cytokine	1054:1061	arg1	TGFβ1					1063:1067	the pro-fibrotic cytokine TGFβ1	1037:1067	the pro-fibrotic cytokine TGFβ1	1037:1067	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	8	51	from	TGFβ1	1182:1186	arg1	fibroblasts					1207:1217	activated colon fibroblasts	1191:1217	activated colon fibroblasts	1191:1217	ZNF281 was downregulated by siRNA transfection, and RNA-sequencing was performed to identify genes regulated by TGFβ1 in activated colon fibroblasts via ZNF281.
36142169	1	52	theme	inflammatory	136:147	arg1	colitis					111:117	ulcerative colitis	100:117	ulcerative colitis (UC)	100:122	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	52	theme	inflammatory	136:147	arg1	disorders					149:157	chronic inflammatory disorders	128:157	chronic inflammatory disorders of the gastrointestinal tract	128:187	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	1	52	theme	inflammatory	136:147	arg1	disease					83:89	Crohn's disease	75:89	Crohn's disease (CD)	75:94	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	11	53	theme	fibroblast	1627:1636	arg1	activation					1638:1647	colon fibroblast activation	1621:1647	colon fibroblast activation	1621:1647	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	10	54	attach	linked	1498:1503	arg1	activation					1519:1528	fibroblast activation	1508:1528	fibroblast activation	1508:1528	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	10	54	attach	linked	1498:1503	arg2	pathways					1489:1496	specific pathways	1480:1496	specific pathways linked to fibroblast activation and differentiation into myofibroblasts	1480:1568	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	10	54	attach	linked	1498:1503	arg1	differentiation					1534:1548	differentiation	1534:1548	differentiation into myofibroblasts	1534:1568	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	8	55	theme	colon	1201:1205	arg1	fibroblasts					1207:1217	activated colon fibroblasts	1191:1217	activated colon fibroblasts	1191:1217	ZNF281 was downregulated by siRNA transfection, and RNA-sequencing was performed to identify genes regulated by TGFβ1 in activated colon fibroblasts via ZNF281.
36142169	6	56	theme	Intestinal	750:759	arg1	fibrosis					761:768	Intestinal fibrosis	750:768	Intestinal fibrosis	750:768	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	1	57	theme	gastrointestinal	166:181	arg1	tract					183:187	the gastrointestinal tract	162:187	the gastrointestinal tract	162:187	Crohn's disease (CD) and ulcerative colitis (UC) are chronic inflammatory disorders of the gastrointestinal tract.
36142169	10	58	from	abrogation	1384:1393	arg1	fibroblasts					1422:1432	TGFβ1-treated fibroblasts	1408:1432	TGFβ1-treated fibroblasts	1408:1432	Moreover, abrogation of ZNF281 in TGFβ1-treated fibroblasts affected the expression of genes belonging to specific pathways linked to fibroblast activation and differentiation into myofibroblasts.
36142169	7	59	theme	cell	1004:1007	arg1	line					1009:1012	the human colon fibroblast cell line	977:1012	the human colon fibroblast cell line CCD-18Co	977:1021	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	2	60	theme	main	218:221	arg1	inflammation					198:209	Chronic inflammation	190:209	Chronic inflammation	190:209	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	2	60	theme	main	218:221	arg1	factor					223:228	the main factor	214:228	the main factor leading to intestinal fibrosis	214:259	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	9	61	from	increase	1255:1262	arg1	colon					1301:1305	in vivo murine fibrotic colon	1277:1305	in vivo murine fibrotic colon	1277:1305	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	9	62	dep	in	1321:1322	arg1	vitro					1324:1328	vitro	1324:1328	vitro	1324:1328	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	11	63	theme	new	1827:1829	arg1	role					1831:1834	a new role	1825:1834	a new role	1825:1834	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	7	64	theme	colon	987:991	arg1	line					1009:1012	the human colon fibroblast cell line	977:1012	the human colon fibroblast cell line CCD-18Co	977:1021	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	5	65	from	role	707:710	arg1	fibrogenesis					736:747	intestinal fibrogenesis	725:747	intestinal fibrogenesis	725:747	The aim of this study is to investigate in vivo and in vitro the role of ZNF281 in intestinal fibrogenesis.
36142169	2	66	theme	intestinal	241:250	arg1	fibrosis					252:259	intestinal fibrosis	241:259	intestinal fibrosis	241:259	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	4	67	theme	zinc-finger	412:422	arg1	regulator					440:448	a zinc-finger transcriptional regulator	410:448	a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer	410:639	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	4	67	theme	zinc-finger	412:422	arg1	ZNF281					400:405	ZNF281	400:405	ZNF281	400:405	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	4	67	theme	zinc-finger	412:422	arg1	transition					510:519	an epithelial-to-mesenchymal transition	481:519	an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor	481:555	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	11	68	theme	matrix	1752:1757	arg1	composition					1765:1775	transcriptionally controlling extracellular matrix (ECM) composition	1708:1775	transcriptionally controlling extracellular matrix (ECM) composition	1708:1775	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	7	69	theme	gut	940:942	arg1	fibrosis					944:951	gut fibrosis	940:951	gut fibrosis	940:951	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	7	70	theme	ZNF281	930:935	arg1	contribution					914:925	The contribution	910:925	The contribution of ZNF281 to gut fibrosis	910:951	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	3	71	theme	underlying	337:346	arg1	unclear					391:397	unclear	391:397	unclear	391:397	Currently, the underlying molecular mechanisms of fibrosis are still unclear.
36142169	3	71	theme	underlying	337:346	arg1	mechanisms					358:367	the underlying molecular mechanisms	333:367	the underlying molecular mechanisms of fibrosis	333:379	Currently, the underlying molecular mechanisms of fibrosis are still unclear.
36142169	9	72	theme	ZNF281	1267:1272	arg1	increase					1255:1262	a marked increase	1246:1262	a marked increase of ZNF281 in in vivo murine fibrotic colon	1246:1305	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	4	73	from	involvement	573:583	arg1	regulation					592:601	the regulation	588:601	the regulation of pluripotency, stemness, and cancer	588:639	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	9	74	theme	in	1277:1278	arg1	colon					1301:1305	in vivo murine fibrotic colon	1277:1305	in vivo murine fibrotic colon	1277:1305	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	3	75	theme	fibrosis	372:379	arg1	unclear					391:397	unclear	391:397	unclear	391:397	Currently, the underlying molecular mechanisms of fibrosis are still unclear.
36142169	3	75	theme	fibrosis	372:379	arg1	mechanisms					358:367	the underlying molecular mechanisms	333:367	the underlying molecular mechanisms of fibrosis	333:379	Currently, the underlying molecular mechanisms of fibrosis are still unclear.
36142169	0	76	theme	Fibroblast	22:31	arg1	Activation					33:42	Colon Fibroblast Activation	16:42	Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis	16:72	ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis.
36142169	9	77	theme	murine	1285:1290	arg1	colon					1301:1305	in vivo murine fibrotic colon	1277:1305	in vivo murine fibrotic colon	1277:1305	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
36142169	2	78	from	factor	223:228	arg1	patients					312:319	CD patients	309:319	CD patients	309:319	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	4	79	theme	-inducing	526:534	arg1	factor					550:555	-inducing transcription factor	526:555	an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor	481:555	ZNF281 is a zinc-finger transcriptional regulator that has been characterized as an epithelial-to-mesenchymal transition (EMT)-inducing transcription factor, suggesting its involvement in the regulation of pluripotency, stemness, and cancer.
36142169	11	80	theme	myofibroblast	1653:1665	arg1	differentiation					1667:1681	myofibroblast differentiation	1653:1681	myofibroblast differentiation	1653:1681	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	0	81	theme	Gut	61:63	arg1	Fibrosis					65:72	TGFβ1-Induced Gut Fibrosis	47:72	TGFβ1-Induced Gut Fibrosis	47:72	ZNF281 Promotes Colon Fibroblast Activation in TGFβ1-Induced Gut Fibrosis.
36142169	2	82	theme	Chronic	190:196	arg1	inflammation					198:209	Chronic inflammation	190:209	Chronic inflammation	190:209	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	2	82	theme	Chronic	190:196	arg1	factor					223:228	the main factor	214:228	the main factor leading to intestinal fibrosis	214:259	Chronic inflammation is the main factor leading to intestinal fibrosis, resulting in recurrent stenosis, especially in CD patients.
36142169	6	83	theme	chronic	812:818	arg1	colitis					820:826	chronic colitis	812:826	chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS)	812:907	Intestinal fibrosis was studied in vivo in C57BL/6J mice with chronic colitis induced by two or three cycles of administration of dextran sulfate sodium (DSS).
36142169	7	84	dep	line	1009:1012	arg1	CCD-18Co					1014:1021	CCD-18Co	1014:1021	the human colon fibroblast cell line CCD-18Co	977:1021	The contribution of ZNF281 to gut fibrosis was studied in vitro in the human colon fibroblast cell line CCD-18Co, activated by the pro-fibrotic cytokine TGFβ1.
36142169	11	85	theme	gut	1868:1870	arg1	fibrosis					1872:1879	gut fibrosis	1868:1879	gut fibrosis	1868:1879	We demonstrated that ZNF281 is a key regulator of colon fibroblast activation and myofibroblast differentiation upon fibrotic stimuli by transcriptionally controlling extracellular matrix (ECM) composition, remodeling, and cell contraction, highlighting a new role in the onset and progression of gut fibrosis.
36142169	9	86	theme	colon	1336:1340	arg1	fibroblasts					1342:1352	in vitro human colon fibroblasts	1321:1352	in vitro human colon fibroblasts activated by TGFβ1	1321:1371	Results showed a marked increase of ZNF281 in in vivo murine fibrotic colon as well as in in vitro human colon fibroblasts activated by TGFβ1.
37110775	5	0	link	N-linked	959:966	arg1	type					968:971	N-linked type	959:971	N-linked type; 75%	959:976	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	4	1	theme	emulsifying	864:874	arg1	properties					876:885	their emulsifying properties	858:885	their emulsifying properties	858:885	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	5	2	link	-linked	1102:1108	arg1	glucans					1110:1116	(β1→3)-linked glucans	1096:1116	(β1→3)-linked glucans	1096:1116	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	10	3	theme	soluble	1805:1811	arg1	mannoproteins					1813:1825	BSY alkali soluble mannoproteins	1794:1825	BSY alkali soluble mannoproteins	1794:1825	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	2	4	theme	structure/function	347:364	arg1	relationships					366:378	structure/function relationships	347:378	structure/function relationships	347:378	The commercial interest in yeast mannoproteins might be boosted considering the consolidation of their properties supported by structure/function relationships.
37110775	4	5	theme	structure/function	583:600	arg1	relationships					602:614	structure/function relationships	583:614	structure/function relationships	583:614	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	6	from	BSY	699:701	arg1	features					685:692	distinct structural features	665:692	distinct structural features from BSY	665:701	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	7	7	theme	O-linked	1377:1384	arg1	mannoproteins					1386:1398	O-linked mannoproteins	1377:1398	O-linked mannoproteins	1377:1398	β-Glucans and O-linked mannoproteins were found to contribute to emulsion stability by preventing Ostwald ripening.
37110775	10	8	theme	water	1843:1847	arg1	additives					1916:1924	additives	1916:1924	additives in sauces	1916:1934	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	8	theme	water	1843:1847	arg1	replacers					1884:1892	replacers	1884:1892	replacers of animal protein	1884:1910	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	8	theme	water	1843:1847	arg1	β-glucans					1859:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	6	9	theme	protein	1164:1170	arg1	content					1172:1178	high protein content	1159:1178	high protein content	1159:1178	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	7	10	link	O-linked	1377:1384	arg1	mannoproteins					1386:1398	O-linked mannoproteins	1377:1398	O-linked mannoproteins	1377:1398	β-Glucans and O-linked mannoproteins were found to contribute to emulsion stability by preventing Ostwald ripening.
37110775	4	11	theme	distinct	665:672	arg1	features					685:692	distinct structural features	665:692	distinct structural features from BSY	665:701	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	10	12	theme	protein	1904:1910	arg1	additives					1916:1924	additives	1916:1924	additives in sauces	1916:1934	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	12	theme	protein	1904:1910	arg1	replacers					1884:1892	replacers	1884:1892	replacers of animal protein	1884:1910	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	12	theme	protein	1904:1910	arg1	β-glucans					1859:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	5	13	theme	O-linked	1061:1068	arg1	type					1070:1073	O-linked type	1061:1073	O-linked type; 55%	1061:1078	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	5	14	theme	branched	935:942	arg1	mannoproteins					944:956	highly branched mannoproteins	928:956	highly branched mannoproteins (N-linked type; 75%)	928:977	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	4	15	theme	mild	741:744	arg1	extraction					729:738	alkaline extraction	720:738	alkaline extraction (mild treatment)	720:755	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	15	theme	mild	741:744	arg1	treatment					746:754	mild treatment	741:754	mild treatment	741:754	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	3	16	from	sources	557:563	arg1	protein					537:543	protein	537:543	protein	537:543	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	16	from	sources	557:563	arg1	replacement					503:513	the replacement	499:513	the replacement of food additives and protein from animal sources	499:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	16	from	sources	557:563	arg1	additives					523:531	food additives	518:531	food additives	518:531	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	10	17	used	used	1876:1879	arg2	β-glucans					1859:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	17	used	used	1876:1879	arg2	replacers					1884:1892	replacers	1884:1892	replacers of animal protein	1884:1910	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	17	used	used	1876:1879	arg2	additives					1916:1924	additives	1916:1924	additives in sauces	1916:1934	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	1	18	theme	spent	119:123	arg1	mannoproteins					137:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties.
37110775	8	19	theme	similar	1571:1577	arg1	emulsifiers					1615:1625	the reference emulsifiers	1601:1625	the reference emulsifiers	1601:1625	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	19	theme	similar	1571:1577	arg1	properties					1587:1596	yet similar texture properties	1567:1596	yet similar texture properties	1567:1596	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	19	theme	similar	1571:1577	arg1	stability					1553:1561	higher stability	1546:1561	higher stability	1546:1561	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	2	20	theme	commercial	224:233	arg1	interest					235:242	The commercial interest	220:242	The commercial interest in yeast mannoproteins	220:265	The commercial interest in yeast mannoproteins might be boosted considering the consolidation of their properties supported by structure/function relationships.
37110775	5	21	link	O-linked	1061:1068	arg1	type					1070:1073	O-linked type	1061:1073	O-linked type; 55%	1061:1078	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	6	22	theme	chain	1277:1281	arg1	mannans					1283:1289	short chain mannans	1271:1289	short chain mannans	1271:1289	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	5	23	theme	short	1040:1044	arg1	chains					1053:1058	short mannan chains	1040:1058	short mannan chains (O-linked type; 55%)	1040:1079	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	5	24	dep	mannoproteins	944:956	arg1	type					968:971	N-linked type	959:971	N-linked type; 75%	959:976	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	9	25	theme	egg	1710:1712	arg1	yolk					1714:1717	egg yolk	1710:1717	egg yolk	1710:1717	When used in a mayonnaise formulation, the BSY extracts were also able to replace egg yolk and modified starch (E1422) at 1/3 of their concentration.
37110775	10	26	from	replacers	1884:1892	arg1	sauces					1929:1934	sauces	1929:1934	sauces	1929:1934	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	3	27	theme	BSY	428:430	arg1	mannoproteins					432:444	extracted BSY mannoproteins	418:444	extracted BSY mannoproteins	418:444	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	6	28	theme	ultraturrax	1341:1351	arg1	stirring					1353:1360	ultraturrax stirring	1341:1360	ultraturrax stirring	1341:1360	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	2	29	from	interest	235:242	arg1	mannoproteins					253:265	yeast mannoproteins	247:265	yeast mannoproteins	247:265	The commercial interest in yeast mannoproteins might be boosted considering the consolidation of their properties supported by structure/function relationships.
37110775	4	30	theme	subcritical	760:770	arg1	extraction					778:787	subcritical water extraction	760:787	subcritical water extraction (SWE)	760:793	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	30	theme	subcritical	760:770	arg1	SWE					790:792	SWE	790:792	SWE	790:792	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	0	31	theme	Spent	9:13	arg1	Polysaccharides					31:45	Brewer's Spent Yeast Cell Wall Polysaccharides	0:45	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean	0:64	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean Label Additives for Mayonnaise Formulation.
37110775	7	32	theme	Ostwald	1461:1467	arg1	ripening					1469:1476	Ostwald ripening	1461:1476	Ostwald ripening	1461:1476	β-Glucans and O-linked mannoproteins were found to contribute to emulsion stability by preventing Ostwald ripening.
37110775	1	33	theme	yeast	125:129	arg1	mannoproteins					137:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties.
37110775	4	34	theme	hard	823:826	arg1	technology					811:820	microwave technology	801:820	microwave technology (hard treatment)	801:837	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	34	theme	hard	823:826	arg1	treatment					828:836	hard treatment	823:836	hard treatment	823:836	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	3	35	theme	clean	451:455	arg1	use					411:413	the use	407:413	the use of extracted BSY mannoproteins	407:444	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	35	theme	clean	451:455	arg1	source					473:478	a clean label and vegan source	449:478	a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources	449:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	6	36	theme	best	1317:1320	arg1	emulsions					1322:1330	the best emulsions	1313:1330	the best emulsions	1313:1330	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	0	37	theme	Cell	21:24	arg1	Polysaccharides					31:45	Brewer's Spent Yeast Cell Wall Polysaccharides	0:45	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean	0:64	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean Label Additives for Mayonnaise Formulation.
37110775	8	38	theme	mayonnaise	1495:1504	arg1	emulsions					1512:1520	mayonnaise model emulsions	1495:1520	mayonnaise model emulsions	1495:1520	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	3	39	theme	ingredients	483:493	arg1	use					411:413	the use	407:413	the use of extracted BSY mannoproteins	407:444	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	39	theme	ingredients	483:493	arg1	source					473:478	a clean label and vegan source	449:478	a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources	449:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	7	40	theme	emulsion	1428:1435	arg1	stability					1437:1445	emulsion stability	1428:1445	emulsion stability	1428:1445	β-Glucans and O-linked mannoproteins were found to contribute to emulsion stability by preventing Ostwald ripening.
37110775	5	41	theme	solubilized	1009:1019	arg1	mannoproteins					1021:1033	SWE solubilized mannoproteins	1005:1033	SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans	1005:1116	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	2	42	theme	properties	323:332	arg1	consolidation					300:312	the consolidation	296:312	the consolidation of their properties supported by structure/function relationships	296:378	The commercial interest in yeast mannoproteins might be boosted considering the consolidation of their properties supported by structure/function relationships.
37110775	8	43	theme	texture	1579:1585	arg1	emulsifiers					1615:1625	the reference emulsifiers	1601:1625	the reference emulsifiers	1601:1625	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	43	theme	texture	1579:1585	arg1	properties					1587:1596	yet similar texture properties	1567:1596	yet similar texture properties	1567:1596	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	43	theme	texture	1579:1585	arg1	stability					1553:1561	higher stability	1546:1561	higher stability	1546:1561	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	1	44	theme	emulsifying	196:206	arg1	properties					208:217	thickening and emulsifying properties	181:217	thickening and emulsifying properties	181:217	Brewer's spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties.
37110775	9	45	theme	concentration	1763:1775	arg1	concentration					1763:1775	their concentration	1757:1775	their concentration	1757:1775	When used in a mayonnaise formulation, the BSY extracts were also able to replace egg yolk and modified starch (E1422) at 1/3 of their concentration.
37110775	9	45	theme	concentration	1763:1775	arg1	1/3					1750:1752	1/3	1750:1752	1/3	1750:1752	When used in a mayonnaise formulation, the BSY extracts were also able to replace egg yolk and modified starch (E1422) at 1/3 of their concentration.
37110775	8	46	theme	BSY	1523:1525	arg1	extracts					1527:1534	BSY extracts	1523:1534	BSY extracts	1523:1534	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	10	47	theme	mannoproteins	1813:1825	arg1	additives					1916:1924	additives	1916:1924	additives in sauces	1916:1934	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	47	theme	mannoproteins	1813:1825	arg1	replacers					1884:1892	replacers	1884:1892	replacers of animal protein	1884:1910	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	47	theme	mannoproteins	1813:1825	arg1	β-glucans					1859:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	6	48	theme	stable	1197:1202	arg1	emulsions					1204:1212	the most stable emulsions	1188:1212	the most stable emulsions obtained by hand shaking	1188:1237	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	8	49	theme	reference	1605:1613	arg1	emulsifiers					1615:1625	the reference emulsifiers	1601:1625	the reference emulsifiers	1601:1625	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	49	theme	reference	1605:1613	arg1	properties					1587:1596	yet similar texture properties	1567:1596	yet similar texture properties	1567:1596	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	49	theme	reference	1605:1613	arg1	stability					1553:1561	higher stability	1546:1561	higher stability	1546:1561	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	10	50	theme	alkali	1798:1803	arg1	mannoproteins					1813:1825	BSY alkali soluble mannoproteins	1794:1825	BSY alkali soluble mannoproteins	1794:1825	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	3	51	from	replacement	503:513	arg1	sources					557:563	animal sources	550:563	animal sources	550:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	52	theme	protein	537:543	arg1	replacement					503:513	the replacement	499:513	the replacement of food additives and protein from animal sources	499:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	4	53	theme	properties	876:885	arg1	assessment					844:853	assessment	844:853	assessment of their emulsifying properties	844:885	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	53	theme	properties	876:885	arg1	technology					811:820	microwave technology	801:820	microwave technology (hard treatment)	801:837	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	53	theme	properties	876:885	arg1	treatment					828:836	hard treatment	823:836	hard treatment	823:836	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	10	54	theme	extracted	1849:1857	arg1	additives					1916:1924	additives	1916:1924	additives in sauces	1916:1934	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	54	theme	extracted	1849:1857	arg1	replacers					1884:1892	replacers	1884:1892	replacers of animal protein	1884:1910	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	10	54	theme	extracted	1849:1857	arg1	β-glucans					1859:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	BSY alkali soluble mannoproteins and subcritical water extracted β-glucans	1794:1867	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	3	55	theme	additives	523:531	arg1	replacement					503:513	the replacement	499:513	the replacement of food additives and protein from animal sources	499:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	4	56	theme	structural	674:683	arg1	features					685:692	distinct structural features	665:692	distinct structural features from BSY	665:701	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	10	57	theme	subcritical	1831:1841	arg1	water					1843:1847	subcritical water	1831:1847	subcritical water	1831:1847	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	6	58	theme	high	1159:1162	arg1	content					1172:1178	high protein content	1159:1178	high protein content	1159:1178	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	4	59	dep	isolating	634:642	arg1	either					704:709	either	704:709	either	704:709	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	3	60	theme	animal	550:555	arg1	sources					557:563	animal sources	550:563	animal sources	550:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	1	61	theme	thickening	181:190	arg1	properties					208:217	thickening and emulsifying properties	181:217	thickening and emulsifying properties	181:217	Brewer's spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties.
37110775	9	62	theme	mayonnaise	1643:1652	arg1	formulation					1654:1664	a mayonnaise formulation	1641:1664	a mayonnaise formulation	1641:1664	When used in a mayonnaise formulation, the BSY extracts were also able to replace egg yolk and modified starch (E1422) at 1/3 of their concentration.
37110775	10	63	theme	animal	1897:1902	arg1	protein					1904:1910	animal protein	1897:1910	animal protein	1897:1910	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	6	64	theme	hand	1226:1229	arg1	shaking					1231:1237	hand shaking	1226:1237	hand shaking	1226:1237	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	4	65	theme	alkaline	720:727	arg1	extraction					729:738	alkaline extraction	720:738	alkaline extraction (mild treatment)	720:755	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	65	theme	alkaline	720:727	arg1	treatment					746:754	mild treatment	741:754	mild treatment	741:754	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	66	dep	performed	621:629	arg1	isolating					634:642	isolating	634:642	isolating polysaccharides with distinct structural features from BSY	634:701	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	2	67	theme	yeast	247:251	arg1	mannoproteins					253:265	yeast mannoproteins	247:265	yeast mannoproteins	247:265	The commercial interest in yeast mannoproteins might be boosted considering the consolidation of their properties supported by structure/function relationships.
37110775	9	68	theme	BSY	1671:1673	arg1	extracts					1675:1682	the BSY extracts	1667:1682	the BSY extracts	1667:1682	When used in a mayonnaise formulation, the BSY extracts were also able to replace egg yolk and modified starch (E1422) at 1/3 of their concentration.
37110775	9	68	theme	BSY	1671:1673	arg1	able					1694:1697	able	1694:1697	able	1694:1697	When used in a mayonnaise formulation, the BSY extracts were also able to replace egg yolk and modified starch (E1422) at 1/3 of their concentration.
37110775	5	69	theme	mannan	1046:1051	arg1	chains					1053:1058	short mannan chains	1040:1058	short mannan chains (O-linked type; 55%)	1040:1079	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	5	70	theme	Alkaline	888:895	arg1	extractions					897:907	Alkaline extractions	888:907	Alkaline extractions	888:907	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	6	71	with	Extracts	1145:1152	arg1	content					1172:1178	high protein content	1159:1178	high protein content	1159:1178	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	1	72	theme	BSY	132:134	arg1	mannoproteins					137:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties.
37110775	1	73	contain	possess	173:179	arg1	mannoproteins					137:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins	110:149	Brewer's spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties.
37110775	1	73	contain	possess	173:179	arg2	properties					208:217	thickening and emulsifying properties	181:217	thickening and emulsifying properties	181:217	Brewer's spent yeast (BSY) mannoproteins have been reported to possess thickening and emulsifying properties.
37110775	5	74	dep	type	968:971	arg1	%					976:976	75%	974:976	N-linked type; 75%	959:976	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	6	75	theme	short	1271:1275	arg1	mannans					1283:1289	short chain mannans	1271:1289	short chain mannans	1271:1289	Extracts with high protein content yielded the most stable emulsions obtained by hand shaking, while the extracts composed of short chain mannans and β-glucans yielded the best emulsions by using ultraturrax stirring.
37110775	4	76	theme	water	772:776	arg1	extraction					778:787	subcritical water extraction	760:787	subcritical water extraction (SWE)	760:793	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	76	theme	water	772:776	arg1	SWE					790:792	SWE	790:792	SWE	790:792	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	5	77	dep	type	1070:1073	arg1	%					1078:1078	55%	1076:1078	O-linked type; 55%	1061:1078	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	5	78	with	mannoproteins	1021:1033	arg1	glucans					1110:1116	(β1→3)-linked glucans	1096:1116	(β1→3)-linked glucans	1096:1116	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	5	78	with	mannoproteins	1021:1033	arg1	chains					1053:1058	short mannan chains	1040:1058	short mannan chains (O-linked type; 55%)	1040:1079	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	5	79	theme	N-linked	959:966	arg1	type					968:971	N-linked type	959:971	N-linked type; 75%	959:976	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	3	80	theme	mannoproteins	432:444	arg1	use					411:413	the use	407:413	the use of extracted BSY mannoproteins	407:444	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	80	theme	mannoproteins	432:444	arg1	source					473:478	a clean label and vegan source	449:478	a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources	449:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	0	81	theme	Yeast	15:19	arg1	Polysaccharides					31:45	Brewer's Spent Yeast Cell Wall Polysaccharides	0:45	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean	0:64	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean Label Additives for Mayonnaise Formulation.
37110775	5	82	theme	-linked	1102:1108	arg1	glucans					1110:1116	(β1→3)-linked glucans	1096:1116	(β1→3)-linked glucans	1096:1116	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	10	83	from	additives	1916:1924	arg1	sauces					1929:1934	sauces	1929:1934	sauces	1929:1934	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	3	84	theme	vegan	467:471	arg1	use					411:413	the use	407:413	the use of extracted BSY mannoproteins	407:444	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	84	theme	vegan	467:471	arg1	source					473:478	a clean label and vegan source	449:478	a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources	449:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	0	85	theme	Wall	26:29	arg1	Polysaccharides					31:45	Brewer's Spent Yeast Cell Wall Polysaccharides	0:45	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean	0:64	Brewer's Spent Yeast Cell Wall Polysaccharides as Vegan and Clean Label Additives for Mayonnaise Formulation.
37110775	3	86	theme	extracted	418:426	arg1	mannoproteins					432:444	extracted BSY mannoproteins	418:444	extracted BSY mannoproteins	418:444	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	8	87	theme	model	1506:1510	arg1	emulsions					1512:1520	mayonnaise model emulsions	1495:1520	mayonnaise model emulsions	1495:1520	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	5	88	dep	chains	1053:1058	arg1	type					1070:1073	O-linked type	1061:1073	O-linked type; 55%	1061:1078	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	3	89	theme	label	457:461	arg1	use					411:413	the use	407:413	the use of extracted BSY mannoproteins	407:444	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	3	89	theme	label	457:461	arg1	source					473:478	a clean label and vegan source	449:478	a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources	449:563	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
37110775	10	90	theme	BSY	1794:1796	arg1	mannoproteins					1813:1825	BSY alkali soluble mannoproteins	1794:1825	BSY alkali soluble mannoproteins	1794:1825	This shows that BSY alkali soluble mannoproteins and subcritical water extracted β-glucans can be used as replacers of animal protein and additives in sauces.
37110775	5	91	theme	β1→3	1097:1100	arg1	glucans					1110:1116	(β1→3)-linked glucans	1096:1116	(β1→3)-linked glucans	1096:1116	Alkaline extractions solubilized mostly highly branched mannoproteins (N-linked type; 75%) and glycogen (25%), while SWE solubilized mannoproteins with short mannan chains (O-linked type; 55%) and (1→4)- and (β1→3)-linked glucans, 33 and 12%, respectively.
37110775	4	92	with	polysaccharides	644:658	arg1	features					685:692	distinct structural features	665:692	distinct structural features from BSY	665:701	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	8	93	theme	higher	1546:1551	arg1	emulsifiers					1615:1625	the reference emulsifiers	1601:1625	the reference emulsifiers	1601:1625	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	93	theme	higher	1546:1551	arg1	properties					1587:1596	yet similar texture properties	1567:1596	yet similar texture properties	1567:1596	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	8	93	theme	higher	1546:1551	arg1	stability					1553:1561	higher stability	1546:1561	higher stability	1546:1561	When applied in mayonnaise model emulsions, BSY extracts presented higher stability and yet similar texture properties as the reference emulsifiers.
37110775	4	94	theme	microwave	801:809	arg1	technology					811:820	microwave technology	801:820	microwave technology (hard treatment)	801:837	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	4	94	theme	microwave	801:809	arg1	treatment					828:836	hard treatment	823:836	hard treatment	823:836	To achieve this, structure/function relationships were performed by isolating polysaccharides with distinct structural features from BSY, either by using alkaline extraction (mild treatment) or subcritical water extraction (SWE) using microwave technology (hard treatment), and assessment of their emulsifying properties.
37110775	3	95	theme	food	518:521	arg1	additives					523:531	food additives	518:531	food additives	518:531	This work aimed to attest the use of extracted BSY mannoproteins as a clean label and vegan source of ingredients for the replacement of food additives and protein from animal sources.
36701112	2	0	used	used	595:598	arg2	design					520:525	a design	518:525	a design of experiments approach based on a response surface methodology	518:589	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	2	1	theme	surface	571:577	arg1	methodology					579:589	a response surface methodology	560:589	a response surface methodology	560:589	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	1	2	theme	selective	122:130	arg1	extraction					132:141	selective extraction	122:141	selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry	122:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	3	3	dep	20	702:703	arg1	to					699:700	to	699:700	to	699:700	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	1	4	theme	residues	226:233	arg1	extraction					132:141	selective extraction	122:141	selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry	122:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	5	5	theme	lignin	1045:1050	arg1	residues					1052:1059	lignin residues	1045:1059	lignin residues	1045:1059	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	2	6	theme	response	562:569	arg1	methodology					579:589	a response surface methodology	560:589	a response surface methodology	560:589	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	5	7	theme	multi-response	982:995	arg1	optimization					997:1008	a multi-response optimization	980:1008	a multi-response optimization	980:1008	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	0	8	theme	Response	75:82	arg1	Methodology					92:102	Response Surface Methodology	75:102	Response Surface Methodology	75:102	Treatment of Pulp Black Liquor with an Ionic Liquid: Optimisation by Using Response Surface Methodology.
36701112	1	9	theme	main	304:307	arg1	wastewater					319:328	the main principal wastewater	300:328	the main principal wastewater of pulping industry	300:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	9	theme	main	304:307	arg1	liquor					287:292	black liquor	281:292	black liquor (BL)	281:297	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	10	theme	polysaccharides	236:250	arg1	extraction					132:141	selective extraction	122:141	selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry	122:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	4	11	theme	organic	858:864	arg1	acids					866:870	organic acids	858:870	organic acids	858:870	Concentration of ligni residues, polysaccharides and organic acids were measured using Folin-Ciocalteu method, the anthrone method and HPLC, respectively.
36701112	1	12	theme	principal	309:317	arg1	wastewater					319:328	the main principal wastewater	300:328	the main principal wastewater of pulping industry	300:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	12	theme	principal	309:317	arg1	liquor					287:292	black liquor	281:292	black liquor (BL)	281:297	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	13	from	liquor	287:292	arg1	present					270:276	present	270:276	present	270:276	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	3	14	theme	liquid	752:757	arg1	factors					618:624	Three continuous factors	601:624	Three continuous factors	601:624	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	14	theme	liquid	752:757	arg1	RV.					759:761	ionic liquid RV.	746:761	ionic liquid RV.	746:761	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	15	theme	volumetric	709:718	arg1	ratio					720:724	volumetric ratio	709:724	volumetric ratio	709:724	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	16	theme	BL	681:682	arg1	factors					618:624	Three continuous factors	601:624	Three continuous factors	601:624	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	16	theme	BL	681:682	arg1	dilution					669:676	dilution	669:676	dilution of BL varying from 5 to 20	669:703	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	16	theme	BL	681:682	arg1	ratio					720:724	volumetric ratio	709:724	volumetric ratio	709:724	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	1	17	from	present	270:276	arg1	liquor					287:292	black liquor	281:292	black liquor (BL)	281:297	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	17	from	present	270:276	arg1	BL					295:296	BL	295:296	BL	295:296	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	17	from	present	270:276	arg1	wastewater					319:328	the main principal wastewater	300:328	the main principal wastewater of pulping industry	300:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	0	18	theme	Surface	84:90	arg1	Methodology					92:102	Response Surface Methodology	75:102	Response Surface Methodology	75:102	Treatment of Pulp Black Liquor with an Ionic Liquid: Optimisation by Using Response Surface Methodology.
36701112	2	19	dep	finding	385:391	arg1	the					368:370	the	368:370	the	368:370	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	2	19	dep	finding	385:391	arg1	objective					372:380	objective	372:380	objective	372:380	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	3	20	dep	19	782:783	arg1	to					779:780	to	779:780	to	779:780	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	1	21	attach	present	270:276	arg1	liquor					287:292	black liquor	281:292	black liquor (BL)	281:297	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	21	attach	present	270:276	arg1	BL					295:296	BL	295:296	BL	295:296	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	21	attach	present	270:276	arg2	residues					226:233	lignin residues	219:233	lignin residues	219:233	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	21	attach	present	270:276	arg1	wastewater					319:328	the main principal wastewater	300:328	the main principal wastewater of pulping industry	300:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	22	theme	ionic	151:155	arg1	chloride					194:201	ionic liquid trihexyltetradecylphosphonium chloride	151:201	ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl)	151:214	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	23	theme	pulping	333:339	arg1	industry					341:348	pulping industry	333:348	pulping industry	333:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	4	24	theme	Folin-Ciocalteu	892:906	arg1	method					908:913	Folin-Ciocalteu method	892:913	Folin-Ciocalteu method	892:913	Concentration of ligni residues, polysaccharides and organic acids were measured using Folin-Ciocalteu method, the anthrone method and HPLC, respectively.
36701112	4	25	theme	acids	866:870	arg1	Concentration					805:817	Concentration	805:817	Concentration of ligni residues, polysaccharides and organic acids	805:870	Concentration of ligni residues, polysaccharides and organic acids were measured using Folin-Ciocalteu method, the anthrone method and HPLC, respectively.
36701112	1	26	theme	liquid	157:162	arg1	chloride					194:201	ionic liquid trihexyltetradecylphosphonium chloride	151:201	ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl)	151:214	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	27	theme	organic	256:262	arg1	acids					264:268	organic acids	256:268	organic acids	256:268	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	5	28	theme	residues	1052:1059	arg1	OA					1110:1111	OA	1110:1111	OA under optimised conditions	1110:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	28	theme	residues	1052:1059	arg1	polysaccharides					1072:1086	polysaccharides	1072:1086	polysaccharides	1072:1086	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	28	theme	residues	1052:1059	arg1	residues					1052:1059	lignin residues	1045:1059	lignin residues	1045:1059	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	28	theme	residues	1052:1059	arg1	%					1067:1067	66.0 %	1062:1067	66.0 % of polysaccharides	1062:1086	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	28	theme	residues	1052:1059	arg1	extraction					1096:1105	no extraction	1093:1105	no extraction of OA under optimised conditions	1093:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	28	theme	residues	1052:1059	arg1	%					1040:1040	84.8 %	1035:1040	84.8 % of lignin residues	1035:1059	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	0	29	dep	Optimisation	53:64	arg1	Treatment					0:8	Treatment	0:8	Treatment of Pulp Black Liquor with an Ionic Liquid	0:50	Treatment of Pulp Black Liquor with an Ionic Liquid: Optimisation by Using Response Surface Methodology.
36701112	1	30	theme	trihexyltetradecylphosphonium	164:192	arg1	chloride					194:201	ionic liquid trihexyltetradecylphosphonium chloride	151:201	ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl)	151:214	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	0	31	theme	Black	18:22	arg1	Liquor					24:29	Pulp Black Liquor	13:29	Pulp Black Liquor	13:29	Treatment of Pulp Black Liquor with an Ionic Liquid: Optimisation by Using Response Surface Methodology.
36701112	1	32	theme	acids	264:268	arg1	extraction					132:141	selective extraction	122:141	selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry	122:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	0	33	theme	Pulp	13:16	arg1	Liquor					24:29	Pulp Black Liquor	13:29	Pulp Black Liquor	13:29	Treatment of Pulp Black Liquor with an Ionic Liquid: Optimisation by Using Response Surface Methodology.
36701112	1	34	theme	present	270:276	arg1	residues					226:233	lignin residues	219:233	lignin residues	219:233	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	4	35	theme	residues	828:835	arg1	Concentration					805:817	Concentration	805:817	Concentration of ligni residues, polysaccharides and organic acids	805:870	Concentration of ligni residues, polysaccharides and organic acids were measured using Folin-Ciocalteu method, the anthrone method and HPLC, respectively.
36701112	1	36	theme	industry	341:348	arg1	wastewater					319:328	the main principal wastewater	300:328	the main principal wastewater of pulping industry	300:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	36	theme	industry	341:348	arg1	liquor					287:292	black liquor	281:292	black liquor (BL)	281:297	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	4	37	theme	anthrone	920:927	arg1	method					929:934	the anthrone method	916:934	the anthrone method	916:934	Concentration of ligni residues, polysaccharides and organic acids were measured using Folin-Ciocalteu method, the anthrone method and HPLC, respectively.
36701112	3	38	dep	13.5	663:666	arg1	to					660:661	to	660:661	to	660:661	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	0	39	theme	Liquor	24:29	arg1	Treatment					0:8	Treatment	0:8	Treatment of Pulp Black Liquor with an Ionic Liquid	0:50	Treatment of Pulp Black Liquor with an Ionic Liquid: Optimisation by Using Response Surface Methodology.
36701112	1	40	theme	black	281:285	arg1	liquor					287:292	black liquor	281:292	black liquor (BL)	281:297	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	40	theme	black	281:285	arg1	BL					295:296	BL	295:296	BL	295:296	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	1	40	theme	black	281:285	arg1	wastewater					319:328	the main principal wastewater	300:328	the main principal wastewater of pulping industry	300:348	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	2	41	theme	lignin	434:439	arg1	resideus					441:448	extract lignin resideus	426:448	extract lignin resideus	426:448	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	2	42	theme	optimized	393:401	arg1	conditions					403:412	optimized conditions	393:412	optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution	393:515	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	2	43	theme	extract	426:432	arg1	resideus					441:448	extract lignin resideus	426:448	extract lignin resideus	426:448	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	2	44	theme	aqueous	500:506	arg1	solution					508:515	aqueous solution	500:515	aqueous solution	500:515	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	3	45	theme	continuous	607:616	arg1	factors					618:624	Three continuous factors	601:624	Three continuous factors	601:624	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	45	theme	continuous	607:616	arg1	dilution					669:676	dilution	669:676	dilution of BL varying from 5 to 20	669:703	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	45	theme	continuous	607:616	arg1	RV.					759:761	ionic liquid RV.	746:761	ionic liquid RV.	746:761	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	45	theme	continuous	607:616	arg1	pH					642:643	initial pH	634:643	initial pH varying from 9 to 13.5	634:666	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	4	46	theme	ligni	822:826	arg1	residues					828:835	ligni residues	822:835	ligni residues	822:835	Concentration of ligni residues, polysaccharides and organic acids were measured using Folin-Ciocalteu method, the anthrone method and HPLC, respectively.
36701112	4	47	theme	polysaccharides	838:852	arg1	Concentration					805:817	Concentration	805:817	Concentration of ligni residues, polysaccharides and organic acids	805:870	Concentration of ligni residues, polysaccharides and organic acids were measured using Folin-Ciocalteu method, the anthrone method and HPLC, respectively.
36701112	5	48	theme	%	1067:1067	arg1	extraction					1021:1030	the extraction	1017:1030	the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions	1017:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	2	49	theme	approach	542:549	arg1	design					520:525	a design	518:525	a design of experiments approach based on a response surface methodology	518:589	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	2	50	theme	experiments	530:540	arg1	approach					542:549	experiments approach	530:549	experiments approach based on a response surface methodology	530:589	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	1	51	dep	[P66614	204:210	arg1	Cl					212:213	Cl	212:213	Cl	212:213	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	0	52	with	Treatment	0:8	arg1	Liquid					45:50	Liquid	45:50	Liquid	45:50	Treatment of Pulp Black Liquor with an Ionic Liquid: Optimisation by Using Response Surface Methodology.
36701112	3	53	theme	liquor	735:740	arg1	factors					618:624	Three continuous factors	601:624	Three continuous factors	601:624	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	53	theme	liquor	735:740	arg1	dilution					669:676	dilution	669:676	dilution of BL varying from 5 to 20	669:703	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	53	theme	liquor	735:740	arg1	ratio					720:724	volumetric ratio	709:724	volumetric ratio	709:724	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	5	54	theme	extraction	1096:1105	arg1	extraction					1021:1030	the extraction	1017:1030	the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions	1017:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	2	55	theme	organic	476:482	arg1	acids					484:488	organic acids	476:488	organic acids	476:488	With the objective of finding optimized conditions allowing to extract lignin resideus while polysaccharides and organic acids remain in aqueous solution, a design of experiments approach based on a response surface methodology was used.
36701112	3	56	theme	initial	634:640	arg1	factors					618:624	Three continuous factors	601:624	Three continuous factors	601:624	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	56	theme	initial	634:640	arg1	pH					642:643	initial pH	634:643	initial pH varying from 9 to 13.5	634:666	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	1	57	dep	chloride	194:201	arg1	[P66614					204:210	[P66614	204:210	[P66614	204:210	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
36701112	5	58	theme	polysaccharides	1072:1086	arg1	OA					1110:1111	OA	1110:1111	OA under optimised conditions	1110:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	58	theme	polysaccharides	1072:1086	arg1	polysaccharides					1072:1086	polysaccharides	1072:1086	polysaccharides	1072:1086	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	58	theme	polysaccharides	1072:1086	arg1	residues					1052:1059	lignin residues	1045:1059	lignin residues	1045:1059	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	58	theme	polysaccharides	1072:1086	arg1	%					1067:1067	66.0 %	1062:1067	66.0 % of polysaccharides	1062:1086	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	58	theme	polysaccharides	1072:1086	arg1	extraction					1096:1105	no extraction	1093:1105	no extraction of OA under optimised conditions	1093:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	58	theme	polysaccharides	1072:1086	arg1	%					1040:1040	84.8 %	1035:1040	84.8 % of lignin residues	1035:1059	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	3	59	theme	ionic	746:750	arg1	factors					618:624	Three continuous factors	601:624	Three continuous factors	601:624	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	3	59	theme	ionic	746:750	arg1	RV.					759:761	ionic liquid RV.	746:761	ionic liquid RV.	746:761	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	5	60	theme	%	1040:1040	arg1	extraction					1021:1030	the extraction	1017:1030	the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions	1017:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	3	61	theme	black	729:733	arg1	liquor					735:740	black liquor	729:740	black liquor	729:740	Three continuous factors, namely initial pH varying from 9 to 13.5, dilution of BL varying from 5 to 20 and volumetric ratio of black liquor vs. ionic liquid RV., varying from 1 to 19, were investigated.
36701112	5	62	theme	OA	1110:1111	arg1	OA					1110:1111	OA	1110:1111	OA under optimised conditions	1110:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	62	theme	OA	1110:1111	arg1	polysaccharides					1072:1086	polysaccharides	1072:1086	polysaccharides	1072:1086	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	62	theme	OA	1110:1111	arg1	residues					1052:1059	lignin residues	1045:1059	lignin residues	1045:1059	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	62	theme	OA	1110:1111	arg1	%					1067:1067	66.0 %	1062:1067	66.0 % of polysaccharides	1062:1086	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	62	theme	OA	1110:1111	arg1	extraction					1096:1105	no extraction	1093:1105	no extraction of OA under optimised conditions	1093:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	62	theme	OA	1110:1111	arg1	%					1040:1040	84.8 %	1035:1040	84.8 % of lignin residues	1035:1059	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	5	63	theme	optimised	1119:1127	arg1	conditions					1129:1138	optimised conditions	1119:1138	optimised conditions	1119:1138	Results showed that a multi-response optimization led to the extraction of 84.8 % of lignin residues, 66.0 % of polysaccharides, and no extraction of OA under optimised conditions.
36701112	1	64	theme	lignin	219:224	arg1	residues					226:233	lignin residues	219:233	lignin residues	219:233	: In this study, selective extraction towards ionic liquid trihexyltetradecylphosphonium chloride ([P66614]Cl) of lignin residues, polysaccharides and organic acids present in black liquor (BL), the main principal wastewater of pulping industry was studied.
35784681	0	0	theme	Antioxidant	77:87	arg1	Activities					89:98	Antioxidant Activities	77:98	Antioxidant Activities	77:98	Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities.
35784681	3	1	theme	chemical	294:301	arg1	properties					303:312	The chemical properties	290:312	The chemical properties of the polysaccharides	290:335	The chemical properties of the polysaccharides were characterised, and their antioxidant activities were evaluated.
35784681	8	2	theme	In	1004:1005	arg1	experiments					1012:1022	In vivo experiments	1004:1022	In vivo experiments in an oxidatively damaged mice model	1004:1059	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	8	3	theme	enzymes	1115:1121	arg1	levels					1093:1098	the levels	1089:1098	the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase,	1089:1192	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	5	4	theme	PCB-1	545:549	arg1	weights					534:540	The molecular weights	520:540	The molecular weights of PCB-1 and PCB2-1	520:560	The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively.
35784681	5	4	theme	PCB-1	545:549	arg1	kDa					598:600	97.8 and 12.8 kDa	584:600	97.8 and 12.8 kDa	584:600	The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively.
35784681	8	5	theme	superoxide	1134:1143	arg1	dismutase					1145:1153	superoxide dismutase	1134:1153	superoxide dismutase	1134:1153	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	1	6	from	Benth	162:166	arg1	fractions					120:128	Two polysaccharide fractions	101:128	Two polysaccharide fractions from Pogostemon cablin (Blanco) Benth.	101:167	Two polysaccharide fractions from Pogostemon cablin (Blanco) Benth.
35784681	1	7	theme	polysaccharide	105:118	arg1	fractions					120:128	Two polysaccharide fractions	101:128	Two polysaccharide fractions from Pogostemon cablin (Blanco) Benth.	101:167	Two polysaccharide fractions from Pogostemon cablin (Blanco) Benth.
35784681	0	8	from	Benth.	48:53	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities.	0:99	Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities.
35784681	5	9	theme	PCB2-1	555:560	arg1	weights					534:540	The molecular weights	520:540	The molecular weights of PCB-1 and PCB2-1	520:560	The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively.
35784681	5	9	theme	PCB2-1	555:560	arg1	kDa					598:600	97.8 and 12.8 kDa	584:600	97.8 and 12.8 kDa	584:600	The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively.
35784681	3	10	theme	polysaccharides	321:335	arg1	properties					303:312	The chemical properties	290:312	The chemical properties of the polysaccharides	290:335	The chemical properties of the polysaccharides were characterised, and their antioxidant activities were evaluated.
35784681	9	11	from	antioxidant	1324:1334	arg1	foods					1350:1354	functional foods	1339:1354	functional foods	1339:1354	These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
35784681	7	12	theme	scavenging	906:915	arg1	activity					917:924	the scavenging activity	902:924	the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities	902:1001	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	9	13	theme	significant	1296:1306	arg1	antioxidant					1324:1334	an antioxidant	1321:1334	an antioxidant in functional foods	1321:1354	These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
35784681	9	13	theme	significant	1296:1306	arg1	potential					1308:1316	significant potential	1296:1316	significant potential	1296:1316	These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
35784681	8	14	dep	serum	1241:1245	arg1	the					1237:1239	the	1237:1239	the	1237:1239	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	8	15	theme	malondialdehyde	1208:1222	arg1	formation					1224:1232	malondialdehyde formation	1208:1232	malondialdehyde formation in the serum and liver	1208:1255	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	3	16	theme	antioxidant	367:377	arg1	activities					379:388	their antioxidant activities	361:388	their antioxidant activities	361:388	The chemical properties of the polysaccharides were characterised, and their antioxidant activities were evaluated.
35784681	4	17	theme	PCB-1	459:463	arg1	content					416:422	The sugar content	406:422	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1	406:475	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	4	17	theme	PCB-1	459:463	arg1	%					503:503	58.74, 90.23 and 88.61%	481:503	58.74, 90.23 and 88.61%	481:503	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	8	18	theme	damaged	1042:1048	arg1	model					1055:1059	an oxidatively damaged mice model	1027:1059	an oxidatively damaged mice model	1027:1059	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	5	19	theme	molecular	524:532	arg1	weights					534:540	The molecular weights	520:540	The molecular weights of PCB-1 and PCB2-1	520:560	The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively.
35784681	5	19	theme	molecular	524:532	arg1	kDa					598:600	97.8 and 12.8 kDa	584:600	97.8 and 12.8 kDa	584:600	The molecular weights of PCB-1 and PCB2-1 were determined to be 97.8 and 12.8 kDa, respectively.
35784681	8	20	from	experiments	1012:1022	arg1	model					1055:1059	an oxidatively damaged mice model	1027:1059	an oxidatively damaged mice model	1027:1059	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	6	21	theme	galacturonic	727:738	arg1	acid					740:743	galacturonic acid	727:743	galacturonic acid	727:743	Monosaccharide composition analysis showed that all the three polysaccharides consisted of mannose, rhamnose, galacturonic acid, galactose, glucose, and arabinose, but with varying molar ratios.
35784681	6	22	theme	composition	632:642	arg1	analysis					644:651	Monosaccharide composition analysis	617:651	Monosaccharide composition analysis	617:651	Monosaccharide composition analysis showed that all the three polysaccharides consisted of mannose, rhamnose, galacturonic acid, galactose, glucose, and arabinose, but with varying molar ratios.
35784681	6	23	theme	molar	798:802	arg1	ratios					804:809	varying molar ratios	790:809	varying molar ratios	790:809	Monosaccharide composition analysis showed that all the three polysaccharides consisted of mannose, rhamnose, galacturonic acid, galactose, glucose, and arabinose, but with varying molar ratios.
35784681	8	24	theme	glutathione	1170:1180	arg1	peroxidase					1182:1191	glutathione peroxidase	1170:1191	glutathione peroxidase	1170:1191	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	6	25	theme	Monosaccharide	617:630	arg1	analysis					644:651	Monosaccharide composition analysis	617:651	Monosaccharide composition analysis	617:651	Monosaccharide composition analysis showed that all the three polysaccharides consisted of mannose, rhamnose, galacturonic acid, galactose, glucose, and arabinose, but with varying molar ratios.
35784681	8	26	theme	mice	1050:1053	arg1	model					1055:1059	an oxidatively damaged mice model	1027:1059	an oxidatively damaged mice model	1027:1059	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	4	27	theme	polysaccharide	437:450	arg1	content					416:422	The sugar content	406:422	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1	406:475	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	4	27	theme	polysaccharide	437:450	arg1	%					503:503	58.74, 90.23 and 88.61%	481:503	58.74, 90.23 and 88.61%	481:503	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	2	28	theme	Sepharose	264:272	arg1	chromatography					274:287	Sepharose chromatography	264:287	Sepharose chromatography	264:287	(P. cablin) (designated as PCB-1 and PCB2-1) were isolated by water extraction and purified by Sepharose chromatography.
35784681	9	29	contain	has	1292:1294	arg2	potential					1308:1316	significant potential	1296:1316	significant potential	1296:1316	These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
35784681	9	29	contain	has	1292:1294	arg1	PCB-1					1286:1290	PCB-1	1286:1290	PCB-1	1286:1290	These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
35784681	9	29	contain	has	1292:1294	arg2	antioxidant					1324:1334	an antioxidant	1321:1334	an antioxidant in functional foods	1321:1354	These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
35784681	4	30	theme	crude	431:435	arg1	polysaccharide					437:450	the crude polysaccharide	427:450	the crude polysaccharide (PCB)	427:456	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	4	30	theme	crude	431:435	arg1	PCB					453:455	PCB	453:455	PCB	453:455	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	8	31	dep	In	1004:1005	arg1	vivo					1007:1010	vivo	1007:1010	vivo	1007:1010	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	9	32	theme	functional	1339:1348	arg1	foods					1350:1354	functional foods	1339:1354	functional foods	1339:1354	These findings suggest that PCB-1 has significant potential as an antioxidant in functional foods.
35784681	6	33	theme	varying	790:796	arg1	ratios					804:809	varying molar ratios	790:809	varying molar ratios	790:809	Monosaccharide composition analysis showed that all the three polysaccharides consisted of mannose, rhamnose, galacturonic acid, galactose, glucose, and arabinose, but with varying molar ratios.
35784681	7	34	theme	ferric-reducing	977:991	arg1	radicals					943:950	hydroxyl radicals	934:950	hydroxyl radicals	934:950	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	7	34	theme	ferric-reducing	977:991	arg1	abilities					993:1001	metal ion-chelating and ferric-reducing abilities	953:1001	metal ion-chelating and ferric-reducing abilities	953:1001	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	7	35	theme	ion-chelating	959:971	arg1	radicals					943:950	hydroxyl radicals	934:950	hydroxyl radicals	934:950	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	7	35	theme	ion-chelating	959:971	arg1	abilities					993:1001	metal ion-chelating and ferric-reducing abilities	953:1001	metal ion-chelating and ferric-reducing abilities	953:1001	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	7	36	theme	antioxidant	861:871	arg1	activities					873:882	significantly high antioxidant activities	842:882	significantly high antioxidant activities	842:882	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	4	37	theme	sugar	410:414	arg1	content					416:422	The sugar content	406:422	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1	406:475	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	4	37	theme	sugar	410:414	arg1	%					503:503	58.74, 90.23 and 88.61%	481:503	58.74, 90.23 and 88.61%	481:503	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	2	38	theme	water	231:235	arg1	extraction					237:246	water extraction	231:246	water extraction	231:246	(P. cablin) (designated as PCB-1 and PCB2-1) were isolated by water extraction and purified by Sepharose chromatography.
35784681	8	39	from	formation	1224:1232	arg1	liver					1251:1255	liver	1251:1255	liver	1251:1255	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	8	39	from	formation	1224:1232	arg1	serum					1241:1245	serum	1241:1245	serum	1241:1245	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	8	40	theme	antioxidant	1103:1113	arg1	enzymes					1115:1121	antioxidant enzymes	1103:1121	antioxidant enzymes	1103:1121	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	8	40	theme	antioxidant	1103:1113	arg1	catalase					1156:1163	catalase	1156:1163	catalase	1156:1163	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	8	40	theme	antioxidant	1103:1113	arg1	dismutase					1145:1153	superoxide dismutase	1134:1153	superoxide dismutase	1134:1153	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	8	40	theme	antioxidant	1103:1113	arg1	peroxidase					1182:1191	glutathione peroxidase	1170:1191	glutathione peroxidase	1170:1191	In vivo experiments in an oxidatively damaged mice model showed that PCB-1 increased the levels of antioxidant enzymes, including superoxide dismutase, catalase, and glutathione peroxidase, and inhibited malondialdehyde formation in the serum and liver.
35784681	7	41	theme	metal	953:957	arg1	radicals					943:950	hydroxyl radicals	934:950	hydroxyl radicals	934:950	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	7	41	theme	metal	953:957	arg1	abilities					993:1001	metal ion-chelating and ferric-reducing abilities	953:1001	metal ion-chelating and ferric-reducing abilities	953:1001	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	7	42	theme	high	856:859	arg1	activities					873:882	significantly high antioxidant activities	842:882	significantly high antioxidant activities	842:882	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	4	43	theme	PCB2-1	470:475	arg1	content					416:422	The sugar content	406:422	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1	406:475	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	4	43	theme	PCB2-1	470:475	arg1	%					503:503	58.74, 90.23 and 88.61%	481:503	58.74, 90.23 and 88.61%	481:503	The sugar content of the crude polysaccharide (PCB), PCB-1, and PCB2-1 was 58.74, 90.23 and 88.61%, respectively.
35784681	0	44	dep	Polysaccharides	0:14	arg1	Characterization					56:71	Characterization	56:71	Characterization	56:71	Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities.
35784681	0	44	dep	Polysaccharides	0:14	arg1	Activities					89:98	Antioxidant Activities	77:98	Antioxidant Activities	77:98	Polysaccharides From Pogostemon cablin (Blanco) Benth.: Characterization and Antioxidant Activities.
35784681	7	45	theme	hydroxyl	934:941	arg1	radicals					943:950	hydroxyl radicals	934:950	hydroxyl radicals	934:950	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
35784681	7	45	theme	hydroxyl	934:941	arg1	abilities					993:1001	metal ion-chelating and ferric-reducing abilities	953:1001	metal ion-chelating and ferric-reducing abilities	953:1001	The polysaccharides exhibited significantly high antioxidant activities in vitro based on the scavenging activity against hydroxyl radicals, metal ion-chelating and ferric-reducing abilities.
36339765	2	0	theme	primary	537:543	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	0	theme	primary	537:543	arg1	characteristics					556:570	different primary structural characteristics	527:570	different primary structural characteristics	527:570	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	1	1	theme	prebiotics	436:445	arg1	activity					447:454	prebiotics activity	436:454	prebiotics activity	436:454	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	2	theme	different	141:149	arg1	compound-enzyme					271:285	compound-enzyme	271:285	compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP)	271:332	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	2	theme	different	141:149	arg1	water					176:180	hot water	172:180	hot water (HW-ASP)	172:189	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	2	theme	different	141:149	arg1	enzyme					199:204	single enzyme	192:204	single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP)	192:264	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	2	theme	different	141:149	arg1	processes					151:159	five different processes	136:159	five different processes	136:159	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	0	3	theme	prebiotic	69:77	arg1	activity					79:86	prebiotic activity	69:86	prebiotic activity	69:86	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	2	4	theme	different	527:535	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	4	theme	different	527:535	arg1	characteristics					556:570	different primary structural characteristics	527:570	different primary structural characteristics	527:570	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	5	5	theme	p	1209:1209	arg1	HW-ASP					1201:1206	HW-ASP	1201:1206	HW-ASP (p < 0.05)	1201:1217	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	5	5	theme	p	1209:1209	arg1	<					1211:1211	p < 0.05	1209:1216	p < 0.05	1209:1216	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	3	6	dep	In	748:749	arg1	vitro					751:755	vitro	751:755	vitro	751:755	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	0	7	theme	soybean	91:97	arg1	polysaccharides					104:118	soybean hull polysaccharides	91:118	soybean hull polysaccharides	91:118	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	0	8	from	Effects	0:6	arg1	properties					54:63	properties	54:63	properties	54:63	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	0	8	from	Effects	0:6	arg1	activity					79:86	prebiotic activity	69:86	prebiotic activity	69:86	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	4	9	dep	probiotics	966:975	arg1	acidophilus					1046:1056	Lactobacillus acidophilus	1032:1056	Lactobacillus acidophilus	1032:1056	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	4	9	dep	probiotics	966:975	arg1	Lactobacillus					1003:1015	Lactobacillus	1003:1015	Lactobacillus	1003:1015	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	4	9	dep	probiotics	966:975	arg1	paracasei					992:1000	Lactobacillus paracasei	978:1000	Lactobacillus paracasei	978:1000	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	2	10	theme	weight	593:598	arg1	size					703:706	potential particle size	684:706	potential particle size	684:706	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	10	theme	weight	593:598	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	10	theme	weight	593:598	arg1	composition					651:661	chemical composition	642:661	chemical composition	642:661	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	10	theme	weight	593:598	arg1	morphology					672:681	surface morphology	664:681	surface morphology	664:681	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	10	theme	weight	593:598	arg1	etc.					709:712	etc.	709:712	etc.	709:712	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	10	theme	weight	593:598	arg1	composition					629:639	monosaccharide composition	614:639	monosaccharide composition	614:639	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	1	11	theme	soybean	356:362	arg1	ASPs					386:389	ASPs	386:389	ASPs	386:389	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	11	theme	soybean	356:362	arg1	polysaccharides					369:383	soybean hull polysaccharides	356:383	soybean hull polysaccharides (ASPs)	356:390	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	0	12	theme	polysaccharides	104:118	arg1	properties					54:63	properties	54:63	properties	54:63	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	0	12	theme	polysaccharides	104:118	arg1	activity					79:86	prebiotic activity	69:86	prebiotic activity	69:86	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	5	13	contain	had	1166:1168	arg2	activity					1187:1194	better prebiotic activity	1170:1194	better prebiotic activity	1170:1194	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	5	13	contain	had	1166:1168	arg1	C-ASP					1095:1099	C-ASP	1095:1099	C-ASP	1095:1099	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	1	14	theme	hull	364:367	arg1	ASPs					386:389	ASPs	386:389	ASPs	386:389	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	14	theme	hull	364:367	arg1	polysaccharides					369:383	soybean hull polysaccharides	356:383	soybean hull polysaccharides (ASPs)	356:390	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	0	15	theme	hull	99:102	arg1	polysaccharides					104:118	soybean hull polysaccharides	91:118	soybean hull polysaccharides	91:118	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	4	16	theme	probiotics	966:975	arg1	growth					956:961	the growth	952:961	the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05)	952:1092	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	1	17	theme	polysaccharides	369:383	arg1	extraction					342:351	the extraction	338:351	the extraction of soybean hull polysaccharides (ASPs)	338:390	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	5	18	theme	better	1170:1175	arg1	activity					1187:1194	better prebiotic activity	1170:1194	better prebiotic activity	1170:1194	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	6	19	contain	have	1308:1311	arg2	potential					1317:1325	the potential to be served as novel probiotics	1313:1358	the potential to be served as novel probiotics	1313:1358	These findings denote that enzyme-assisted polysaccharides extracted from soybean hulls have the potential to be served as novel probiotics.
36339765	6	19	contain	have	1308:1311	arg1	polysaccharides					1263:1277	enzyme-assisted polysaccharides	1247:1277	enzyme-assisted polysaccharides extracted from soybean hulls	1247:1306	These findings denote that enzyme-assisted polysaccharides extracted from soybean hulls have the potential to be served as novel probiotics.
36339765	1	20	theme	polysaccharides	464:478	arg1	characterization					415:430	the characterization	411:430	the characterization	411:430	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	20	theme	polysaccharides	464:478	arg1	activity					447:454	prebiotics activity	436:454	prebiotics activity	436:454	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	21	theme	hot	172:174	arg1	HW-ASP					183:188	HW-ASP	183:188	HW-ASP	183:188	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	21	theme	hot	172:174	arg1	water					176:180	hot water	172:180	hot water (HW-ASP)	172:189	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	5	22	theme	short-chain	1130:1140	arg1	SCFAs					1155:1159	SCFAs	1155:1159	SCFAs	1155:1159	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	5	22	theme	short-chain	1130:1140	arg1	acids					1148:1152	total short-chain fatty acids	1124:1152	total short-chain fatty acids (SCFAs)	1124:1160	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	2	23	theme	structural	545:554	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	23	theme	structural	545:554	arg1	characteristics					556:570	different primary structural characteristics	527:570	different primary structural characteristics	527:570	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	4	24	theme	p	1084:1084	arg1	<					1086:1086	p < 0.05	1084:1091	p < 0.05	1084:1091	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	4	24	theme	p	1084:1084	arg1	fermentation					1070:1081	the fermentation	1066:1081	the fermentation (p < 0.05)	1066:1092	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	1	25	dep	cellulase	207:215	arg1	pectinase					218:226	pectinase	218:226	pectinase	218:226	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	25	dep	cellulase	207:215	arg1	PE-ASP					247:252	PE-ASP	247:252	PE-ASP	247:252	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	25	dep	cellulase	207:215	arg1	P-ASP					259:263	P-ASP	259:263	P-ASP	259:263	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	25	dep	cellulase	207:215	arg1	C-ASP					240:244	C-ASP	240:244	C-ASP	240:244	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	25	dep	cellulase	207:215	arg1	papain					232:237	papain	232:237	papain	232:237	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	0	26	theme	enzyme	21:26	arg1	methods					39:45	different enzyme extraction methods	11:45	different enzyme extraction methods	11:45	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	5	27	theme	fatty	1142:1146	arg1	SCFAs					1155:1159	SCFAs	1155:1159	SCFAs	1155:1159	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	5	27	theme	fatty	1142:1146	arg1	acids					1148:1152	total short-chain fatty acids	1124:1152	total short-chain fatty acids (SCFAs)	1124:1160	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	6	28	theme	soybean	1294:1300	arg1	hulls					1302:1306	soybean hulls	1294:1306	soybean hulls	1294:1306	These findings denote that enzyme-assisted polysaccharides extracted from soybean hulls have the potential to be served as novel probiotics.
36339765	2	29	theme	monosaccharide	614:627	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	29	theme	monosaccharide	614:627	arg1	composition					629:639	monosaccharide composition	614:639	monosaccharide composition	614:639	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	30	theme	molecular	583:591	arg1	size					703:706	potential particle size	684:706	potential particle size	684:706	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	30	theme	molecular	583:591	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	30	theme	molecular	583:591	arg1	composition					651:661	chemical composition	642:661	chemical composition	642:661	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	30	theme	molecular	583:591	arg1	morphology					672:681	surface morphology	664:681	surface morphology	664:681	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	30	theme	molecular	583:591	arg1	etc.					709:712	etc.	709:712	etc.	709:712	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	30	theme	molecular	583:591	arg1	composition					629:639	monosaccharide composition	614:639	monosaccharide composition	614:639	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	0	31	theme	different	11:19	arg1	methods					39:45	different enzyme extraction methods	11:45	different enzyme extraction methods	11:45	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	5	32	theme	acids	1148:1152	arg1	levels					1114:1119	the levels	1110:1119	the levels of total short-chain fatty acids (SCFAs)	1110:1160	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	1	33	theme	papain	310:315	arg1	3:3:1					319:323	papain = 3:3:1	310:323	cellulose: pectinase: papain = 3:3:1; CE-ASP	288:331	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	0	34	dep	properties	54:63	arg1	the					50:52	the	50:52	the	50:52	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	2	35	theme	functional	729:738	arg1	groups					740:745	similar functional groups	721:745	similar functional groups	721:745	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	0	36	theme	methods	39:45	arg1	Effects					0:6	Effects	0:6	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides	0:118	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	1	37	theme	=	317:317	arg1	3:3:1					319:323	papain = 3:3:1	310:323	cellulose: pectinase: papain = 3:3:1; CE-ASP	288:331	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	2	38	theme	similar	721:727	arg1	groups					740:745	similar functional groups	721:745	similar functional groups	721:745	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	0	39	theme	extraction	28:37	arg1	methods					39:45	different enzyme extraction methods	11:45	different enzyme extraction methods	11:45	Effects of different enzyme extraction methods on the properties and prebiotic activity of soybean hull polysaccharides.
36339765	4	40	theme	acidifying	919:928	arg1	activity					930:937	the acidifying activity	915:937	the acidifying activity	915:937	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	6	41	theme	enzyme-assisted	1247:1261	arg1	polysaccharides					1263:1277	enzyme-assisted polysaccharides	1247:1277	enzyme-assisted polysaccharides extracted from soybean hulls	1247:1306	These findings denote that enzyme-assisted polysaccharides extracted from soybean hulls have the potential to be served as novel probiotics.
36339765	1	42	dep	enzyme	199:204	arg1	cellulase					207:215	cellulase	207:215	cellulase	207:215	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	3	43	contain	had	798:800	arg1	C-ASP					792:796	C-ASP	792:796	C-ASP	792:796	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	3	43	contain	had	798:800	arg2	resistance					809:818	strong resistance	802:818	strong resistance to gastric juice hydrolysis and α-amylase	802:860	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	2	44	theme	surface	664:670	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	44	theme	surface	664:670	arg1	morphology					672:681	surface morphology	664:681	surface morphology	664:681	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	6	45	theme	novel	1343:1347	arg1	probiotics					1349:1358	novel probiotics	1343:1358	novel probiotics	1343:1358	These findings denote that enzyme-assisted polysaccharides extracted from soybean hulls have the potential to be served as novel probiotics.
36339765	1	46	dep	cellulose	288:296	arg1	3:3:1					319:323	papain = 3:3:1	310:323	cellulose: pectinase: papain = 3:3:1; CE-ASP	288:331	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	46	dep	cellulose	288:296	arg1	pectinase					299:307	pectinase	299:307	cellulose: pectinase: papain = 3:3:1; CE-ASP	288:331	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	1	46	dep	cellulose	288:296	arg1	CE-ASP					326:331	CE-ASP	326:331	cellulose: pectinase: papain = 3:3:1; CE-ASP	288:331	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	4	47	dep	Lactobacillus	1003:1015	arg1	rhamnosus					1017:1025	Lactobacillus rhamnosus	1003:1025	Lactobacillus rhamnosus	1003:1025	Furthermore, C-ASP elevated the acidifying activity and promoted the growth of probiotics (Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus acidophilus) during the fermentation (p < 0.05).
36339765	5	48	theme	total	1124:1128	arg1	SCFAs					1155:1159	SCFAs	1155:1159	SCFAs	1155:1159	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	5	48	theme	total	1124:1128	arg1	acids					1148:1152	total short-chain fatty acids	1124:1152	total short-chain fatty acids (SCFAs)	1124:1160	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	2	49	theme	chemical	642:649	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	49	theme	chemical	642:649	arg1	composition					651:661	chemical composition	642:661	chemical composition	642:661	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	50	contain	possessed	517:525	arg1	polysaccharides					501:515	These polysaccharides	495:515	These polysaccharides	495:515	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	50	contain	possessed	517:525	arg2	characteristics					556:570	different primary structural characteristics	527:570	different primary structural characteristics	527:570	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	50	contain	possessed	517:525	arg2	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	1	51	theme	single	192:197	arg1	enzyme					199:204	single enzyme	192:204	single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP)	192:264	In this study, five different processes, including hot water (HW-ASP), single enzyme (cellulase, pectinase and papain; C-ASP, PE-ASP, and P-ASP), and compound-enzyme (cellulose: pectinase: papain = 3:3:1; CE-ASP) for the extraction of soybean hull polysaccharides (ASPs) were employed, and the characterization and prebiotics activity of five polysaccharides were analyzed.
36339765	3	52	dep	juice	831:835	arg1	hydrolysis					837:846	hydrolysis	837:846	hydrolysis	837:846	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	3	52	dep	juice	831:835	arg1	α-amylase					852:860	α-amylase	852:860	α-amylase	852:860	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	3	53	theme	strong	802:807	arg1	resistance					809:818	strong resistance	802:818	strong resistance to gastric juice hydrolysis and α-amylase	802:860	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	5	54	theme	prebiotic	1177:1185	arg1	activity					1187:1194	better prebiotic activity	1170:1194	better prebiotic activity	1170:1194	C-ASP improved the levels of total short-chain fatty acids (SCFAs) and had better prebiotic activity than HW-ASP (p < 0.05).
36339765	2	55	theme	particle	694:701	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	55	theme	particle	694:701	arg1	size					703:706	potential particle size	684:706	potential particle size	684:706	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	3	56	theme	gastric	823:829	arg1	juice					831:835	gastric juice hydrolysis and α-amylase	823:860	gastric juice hydrolysis and α-amylase	823:860	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	3	57	theme	In	748:749	arg1	assay					771:775	In vitro digestibility assay	748:775	In vitro digestibility assay	748:775	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
36339765	2	58	theme	potential	684:692	arg1	distribution					600:611	molecular weight distribution	583:611	molecular weight distribution	583:611	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	2	58	theme	potential	684:692	arg1	size					703:706	potential particle size	684:706	potential particle size	684:706	These polysaccharides possessed different primary structural characteristics, including molecular weight distribution, monosaccharide composition, chemical composition, surface morphology, potential particle size, etc., while similar functional groups.
36339765	3	59	theme	digestibility	757:769	arg1	assay					771:775	In vitro digestibility assay	748:775	In vitro digestibility assay	748:775	In vitro digestibility assay indicated that C-ASP had strong resistance to gastric juice hydrolysis and α-amylase as compared with HW-ASP.
37188318	4	0	theme	counterparts	908:919	arg1	composition					833:843	the polyphenolic composition	816:843	the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts	816:919	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	9	1	theme	non-covalent	1973:1984	arg1	structures					1986:1995	those non-covalent structures	1967:1995	those non-covalent structures	1967:1995	The formation of those non-covalent structures may affect red wine color stability and astringency.
37188318	3	2	theme	pigment	692:698	arg1	analyses					700:707	polymeric pigment analyses	682:707	polymeric pigment analyses	682:707	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	2	3	theme	pectic	354:359	arg1	polysaccharides					361:375	pectic polysaccharides	354:375	pectic polysaccharides	354:375	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	6	4	theme	6-13	1351:1354	arg1	%					1355:1355	6-13%	1351:1355	6-13%	1351:1355	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	0	5	link	Grape-derived	0:12	arg1	polysaccharides					21:35	Grape-derived pectic polysaccharides	0:35	Grape-derived pectic polysaccharides	0:35	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	9	6	theme	red	2008:2010	arg1	stability					2023:2031	red wine color stability	2008:2031	red wine color stability	2008:2031	The formation of those non-covalent structures may affect red wine color stability and astringency.
37188318	4	7	theme	corresponding	874:886	arg1	counterparts					908:919	their corresponding polysaccharide-free counterparts	868:919	their corresponding polysaccharide-free counterparts	868:919	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	9	8	theme	color	2017:2021	arg1	stability					2023:2031	red wine color stability	2008:2031	red wine color stability	2008:2031	The formation of those non-covalent structures may affect red wine color stability and astringency.
37188318	3	9	theme	polysaccharides	570:584	arg1	impact					647:652	their impact	641:652	their impact on anthocyanin, tannin, and polymeric pigment analyses	641:707	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	3	9	theme	polysaccharides	570:584	arg1	composition					544:554	the composition	540:554	the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines	540:635	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	1	10	theme	red	196:198	arg1	wine					200:203	red wine	196:203	red wine	196:203	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	7	11	theme	wine	1695:1698	arg1	color					1700:1704	red wine color	1691:1704	red wine color	1691:1704	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	8	12	theme	aggregates	1859:1868	arg1	formation					1819:1827	the formation	1815:1827	the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments	1815:1947	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	3	13	theme	Cabernet	612:619	arg1	wines					631:635	commercially available Cabernet Sauvignon wines	589:635	commercially available Cabernet Sauvignon wines	589:635	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	7	14	theme	increased	1437:1445	arg1	precipitability					1447:1461	the increased precipitability	1433:1461	the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively	1433:1541	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	5	15	theme	wall	953:956	arg1	fragments					958:966	the cell wall fragments	944:966	the cell wall fragments	944:966	The results show that the cell wall fragments enhance the spectral absorbance of anthocyanins by facilitating anthocyanin self-association, leading to a co-pigmentation-like effect.
37188318	6	16	theme	protein	1296:1302	arg1	precipitation					1304:1316	protein precipitation	1296:1316	protein precipitation of tannins, which was reduced by 6-13%	1296:1355	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	2	17	with	features	464:471	arg1	polyphenols					505:515	the polyphenols	501:515	the polyphenols	501:515	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	7	18	theme	esterification	1410:1423	arg1	degree					1400:1405	a high degree	1393:1405	a high degree of esterification	1393:1423	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	3	19	theme	wines	631:635	arg1	polysaccharides					570:584	the pectic polysaccharides	559:584	the pectic polysaccharides of commercially available Cabernet Sauvignon wines	559:635	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	2	20	from	quality	428:434	arg1	extent					390:395	the extent	386:395	the extent of their influence on red wine quality	386:434	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	0	21	theme	Cabernet	81:88	arg1	wines					104:108	Cabernet Sauvignon red wines	81:108	Cabernet Sauvignon red wines	81:108	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	1	22	theme	essential	161:169	arg1	pigments					148:155	polymeric pigments	138:155	polymeric pigments	138:155	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	22	theme	essential	161:169	arg1	anthocyanins					120:131	anthocyanins	120:131	anthocyanins	120:131	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	22	theme	essential	161:169	arg1	constituents					180:191	essential phenolic constituents	161:191	essential phenolic constituents of red wine	161:203	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	22	theme	essential	161:169	arg1	Tannins					111:117	Tannins	111:117	Tannins	111:117	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	7	23	theme	polymeric	1630:1638	arg1	pigments					1640:1647	precipitable polymeric pigments	1617:1647	precipitable polymeric pigments that are responsible for the longevity of red wine color	1617:1704	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	8	24	with	interactions	1764:1775	arg1	polysaccharides					1786:1800	the polysaccharides	1782:1800	the polysaccharides	1782:1800	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	4	25	theme	wines	792:796	arg1	preparation					753:763	preparation	753:763	preparation of polysaccharide deprived wines	753:796	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	4	25	theme	wines	792:796	arg1	comparison					802:811	comparison	802:811	comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts	802:919	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	3	26	from	composition	544:554	arg1	anthocyanin					657:667	anthocyanin	657:667	anthocyanin	657:667	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	3	26	from	composition	544:554	arg1	analyses					700:707	polymeric pigment analyses	682:707	polymeric pigment analyses	682:707	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	3	26	from	composition	544:554	arg1	tannin					670:675	tannin	670:675	tannin	670:675	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	0	27	theme	red	100:102	arg1	wines					104:108	Cabernet Sauvignon red wines	81:108	Cabernet Sauvignon red wines	81:108	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	8	28	theme	formed	1920:1925	arg1	pigments					1940:1947	the covalently formed precipitable pigments	1905:1947	the covalently formed precipitable pigments	1905:1947	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	5	29	theme	anthocyanin	1032:1042	arg1	self-association					1044:1059	anthocyanin self-association	1032:1059	anthocyanin self-association	1032:1059	The results show that the cell wall fragments enhance the spectral absorbance of anthocyanins by facilitating anthocyanin self-association, leading to a co-pigmentation-like effect.
37188318	0	30	theme	Grape-derived	0:12	arg1	polysaccharides					21:35	Grape-derived pectic polysaccharides	0:35	Grape-derived pectic polysaccharides	0:35	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	7	31	dep	1.9	1525:1527	arg1	to					1522:1523	to	1522:1523	to	1522:1523	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	5	32	theme	spectral	980:987	arg1	absorbance					989:998	the spectral absorbance	976:998	the spectral absorbance of anthocyanins	976:1014	The results show that the cell wall fragments enhance the spectral absorbance of anthocyanins by facilitating anthocyanin self-association, leading to a co-pigmentation-like effect.
37188318	7	33	theme	high	1395:1398	arg1	degree					1400:1405	a high degree	1393:1405	a high degree of esterification	1393:1423	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	4	34	theme	polysaccharide	768:781	arg1	wines					792:796	polysaccharide deprived wines	768:796	polysaccharide deprived wines	768:796	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	2	35	theme	wine	423:426	arg1	quality					428:434	red wine quality	419:434	red wine quality	419:434	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	6	36	theme	weight	1118:1123	arg1	pectins					1125:1131	Low molecular weight pectins	1104:1131	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification	1104:1221	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	6	37	theme	molecular	1108:1116	arg1	weight					1118:1123	Low molecular weight	1104:1123	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification	1104:1221	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	7	38	dep	32.4	1509:1512	arg1	to					1506:1507	to	1506:1507	to	1506:1507	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	5	39	theme	co-pigmentation-like	1075:1094	arg1	effect					1096:1101	a co-pigmentation-like effect	1073:1101	a co-pigmentation-like effect	1073:1101	The results show that the cell wall fragments enhance the spectral absorbance of anthocyanins by facilitating anthocyanin self-association, leading to a co-pigmentation-like effect.
37188318	2	40	from	extent	390:395	arg1	quality					428:434	red wine quality	419:434	red wine quality	419:434	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	7	41	theme	weight	1373:1378	arg1	pectins					1380:1386	High molecular weight pectins	1358:1386	High molecular weight pectins with a high degree of esterification	1358:1423	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	7	42	theme	High	1358:1361	arg1	weight					1373:1378	High molecular weight	1358:1378	High molecular weight pectins with a high degree of esterification	1358:1423	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	3	43	theme	polymeric	682:690	arg1	analyses					700:707	polymeric pigment analyses	682:707	polymeric pigment analyses	682:707	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	6	44	with	pectins	1125:1131	arg1	degree					1198:1203	a low degree	1192:1203	a low degree of esterification	1192:1221	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	6	45	theme	low	1194:1196	arg1	degree					1198:1203	a low degree	1192:1203	a low degree of esterification	1192:1221	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	6	46	theme	Low	1104:1106	arg1	weight					1118:1123	Low molecular weight	1104:1123	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification	1104:1221	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	4	47	theme	composition	833:843	arg1	preparation					753:763	preparation	753:763	preparation of polysaccharide deprived wines	753:796	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	4	47	theme	composition	833:843	arg1	comparison					802:811	comparison	802:811	comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts	802:919	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	1	48	theme	color	233:237	arg1	stability					239:247	color stability	233:247	color stability	233:247	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	7	49	from	incorporation	1568:1580	arg1	tannins					1601:1607	tannins	1601:1607	tannins	1601:1607	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	7	50	theme	1.9	1525:1527	arg1	factor					1492:1497	a factor	1490:1497	a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively	1490:1541	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	4	51	theme	polysaccharide-free	888:906	arg1	counterparts					908:919	their corresponding polysaccharide-free counterparts	868:919	their corresponding polysaccharide-free counterparts	868:919	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	6	52	theme	polygalacturonic	1164:1179	arg1	acids					1181:1185	polygalacturonic acids	1164:1185	polygalacturonic acids	1164:1185	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	9	53	theme	structures	1986:1995	arg1	formation					1954:1962	The formation	1950:1962	The formation of those non-covalent structures	1950:1995	The formation of those non-covalent structures may affect red wine color stability and astringency.
37188318	3	54	from	impact	647:652	arg1	anthocyanin					657:667	anthocyanin	657:667	anthocyanin	657:667	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	3	54	from	impact	647:652	arg1	analyses					700:707	polymeric pigment analyses	682:707	polymeric pigment analyses	682:707	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	3	54	from	impact	647:652	arg1	tannin					670:675	tannin	670:675	tannin	670:675	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	8	55	theme	due	1753:1755	arg1	pigments					1744:1751	the pigments	1740:1751	the pigments due to the interactions with the polysaccharides	1740:1800	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	1	56	theme	mouthfeel	254:262	arg1	properties					264:273	mouthfeel properties	254:273	mouthfeel properties	254:273	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	3	57	theme	pectic	563:568	arg1	polysaccharides					570:584	the pectic polysaccharides	559:584	the pectic polysaccharides of commercially available Cabernet Sauvignon wines	559:635	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	1	58	theme	phenolic	171:178	arg1	pigments					148:155	polymeric pigments	138:155	polymeric pigments	138:155	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	58	theme	phenolic	171:178	arg1	anthocyanins					120:131	anthocyanins	120:131	anthocyanins	120:131	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	58	theme	phenolic	171:178	arg1	constituents					180:191	essential phenolic constituents	161:191	essential phenolic constituents of red wine	161:203	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	58	theme	phenolic	171:178	arg1	Tannins					111:117	Tannins	111:117	Tannins	111:117	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	7	59	theme	32.4	1509:1512	arg1	factor					1492:1497	a factor	1490:1497	a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively	1490:1541	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	4	60	theme	wines	858:862	arg1	composition					833:843	the polyphenolic composition	816:843	the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts	816:919	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	6	61	theme	soluble	1243:1249	arg1	complexes					1251:1259	soluble complexes	1243:1259	soluble complexes with anthocyanins	1243:1277	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	9	62	theme	wine	2012:2015	arg1	stability					2023:2031	red wine color stability	2008:2031	red wine color stability	2008:2031	The formation of those non-covalent structures may affect red wine color stability and astringency.
37188318	6	63	with	complexes	1251:1259	arg1	anthocyanins					1266:1277	anthocyanins	1266:1277	anthocyanins	1266:1277	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	2	64	theme	compounds	315:323	arg1	behavior					297:304	The behavior	293:304	The behavior of these compounds	293:323	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	7	65	theme	color	1700:1704	arg1	longevity					1678:1686	the longevity	1674:1686	the longevity of red wine color	1674:1704	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	1	66	theme	wine	200:203	arg1	pigments					148:155	polymeric pigments	138:155	polymeric pigments	138:155	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	66	theme	wine	200:203	arg1	anthocyanins					120:131	anthocyanins	120:131	anthocyanins	120:131	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	66	theme	wine	200:203	arg1	constituents					180:191	essential phenolic constituents	161:191	essential phenolic constituents of red wine	161:203	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	66	theme	wine	200:203	arg1	Tannins					111:117	Tannins	111:117	Tannins	111:117	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	7	67	theme	red	1691:1693	arg1	color					1700:1704	red wine color	1691:1704	red wine color	1691:1704	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	7	68	theme	tannins	1479:1485	arg1	precipitability					1447:1461	the increased precipitability	1433:1461	the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively	1433:1541	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	7	69	with	pectins	1380:1386	arg1	degree					1400:1405	a high degree	1393:1405	a high degree of esterification	1393:1423	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	3	70	theme	available	602:610	arg1	wines					631:635	commercially available Cabernet Sauvignon wines	589:635	commercially available Cabernet Sauvignon wines	589:635	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	8	71	theme	comparable	1880:1889	arg1	properties					1891:1900	comparable properties	1880:1900	comparable properties to the covalently formed precipitable pigments	1880:1947	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	7	72	theme	pigments	1466:1473	arg1	precipitability					1447:1461	the increased precipitability	1433:1461	the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively	1433:1541	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	5	73	theme	cell	948:951	arg1	fragments					958:966	the cell wall fragments	944:966	the cell wall fragments	944:966	The results show that the cell wall fragments enhance the spectral absorbance of anthocyanins by facilitating anthocyanin self-association, leading to a co-pigmentation-like effect.
37188318	8	74	theme	pigmented	1832:1840	arg1	aggregates					1859:1868	pigmented yet non-covalent aggregates	1832:1868	pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments	1832:1947	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	3	75	theme	Sauvignon	621:629	arg1	wines					631:635	commercially available Cabernet Sauvignon wines	589:635	commercially available Cabernet Sauvignon wines	589:635	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	8	76	theme	non-covalent	1846:1857	arg1	aggregates					1859:1868	pigmented yet non-covalent aggregates	1832:1868	pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments	1832:1947	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	1	77	theme	polymeric	138:146	arg1	pigments					148:155	polymeric pigments	138:155	polymeric pigments	138:155	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	77	theme	polymeric	138:146	arg1	anthocyanins					120:131	anthocyanins	120:131	anthocyanins	120:131	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	77	theme	polymeric	138:146	arg1	constituents					180:191	essential phenolic constituents	161:191	essential phenolic constituents of red wine	161:203	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	1	77	theme	polymeric	138:146	arg1	Tannins					111:117	Tannins	111:117	Tannins	111:117	Tannins, anthocyanins, and polymeric pigments are essential phenolic constituents of red wine because they provide color, color stability, and mouthfeel properties like astringency.
37188318	0	78	theme	Sauvignon	90:98	arg1	wines					104:108	Cabernet Sauvignon red wines	81:108	Cabernet Sauvignon red wines	81:108	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	7	79	theme	precipitable	1617:1628	arg1	pigments					1640:1647	precipitable polymeric pigments	1617:1647	precipitable polymeric pigments that are responsible for the longevity of red wine color	1617:1704	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	0	80	theme	wines	104:108	arg1	tannin					47:52	tannin	47:52	tannin	47:52	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	0	80	theme	wines	104:108	arg1	composition					66:76	pigment composition	58:76	pigment composition	58:76	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	2	81	with	interactions	483:494	arg1	polyphenols					505:515	the polyphenols	501:515	the polyphenols	501:515	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	5	82	theme	anthocyanins	1003:1014	arg1	absorbance					989:998	the spectral absorbance	976:998	the spectral absorbance of anthocyanins	976:1014	The results show that the cell wall fragments enhance the spectral absorbance of anthocyanins by facilitating anthocyanin self-association, leading to a co-pigmentation-like effect.
37188318	4	83	theme	deprived	783:790	arg1	wines					792:796	polysaccharide deprived wines	768:796	polysaccharide deprived wines	768:796	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	0	84	theme	pectic	14:19	arg1	polysaccharides					21:35	Grape-derived pectic polysaccharides	0:35	Grape-derived pectic polysaccharides	0:35	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	8	85	theme	precipitable	1927:1938	arg1	pigments					1940:1947	the covalently formed precipitable pigments	1905:1947	the covalently formed precipitable pigments	1905:1947	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	2	86	theme	red	419:421	arg1	quality					428:434	red wine quality	419:434	red wine quality	419:434	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	7	87	theme	molecular	1363:1371	arg1	weight					1373:1378	High molecular weight	1358:1378	High molecular weight pectins with a high degree of esterification	1358:1423	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	2	88	theme	influence	406:414	arg1	extent					390:395	the extent	386:395	the extent of their influence on red wine quality	386:434	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	0	89	theme	pigment	58:64	arg1	composition					66:76	pigment composition	58:76	pigment composition	58:76	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	7	90	theme	anthocyanins	1585:1596	arg1	incorporation					1568:1580	the incorporation	1564:1580	the incorporation of anthocyanins in tannins	1564:1607	High molecular weight pectins with a high degree of esterification lead to the increased precipitability of pigments and tannins by a factor of 1.3 to 32.4 and 1.1 to 1.9, respectively, seemingly impairing the incorporation of anthocyanins in tannins to form precipitable polymeric pigments that are responsible for the longevity of red wine color.
37188318	0	91	dep	tannin	47:52	arg1	the					43:45	the	43:45	the	43:45	Grape-derived pectic polysaccharides alter the tannin and pigment composition of Cabernet Sauvignon red wines.
37188318	6	92	theme	esterification	1208:1221	arg1	degree					1198:1203	a low degree	1192:1203	a low degree of esterification	1192:1221	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	2	93	theme	structural	453:462	arg1	features					464:471	their structural features	447:471	their structural features	447:471	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	8	94	theme	increased	1711:1719	arg1	precipitability					1721:1735	The increased precipitability	1707:1735	The increased precipitability of the pigments due to the interactions with the polysaccharides	1707:1800	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
37188318	6	95	theme	tannins	1321:1327	arg1	precipitation					1304:1316	protein precipitation	1296:1316	protein precipitation of tannins, which was reduced by 6-13%	1296:1355	Low molecular weight pectins like rhamnogalacturonan II and polygalacturonic acids with a low degree of esterification are assumed to form soluble complexes with anthocyanins and also prevent protein precipitation of tannins, which was reduced by 6-13%.
37188318	2	96	from	influence	406:414	arg1	quality					428:434	red wine quality	419:434	red wine quality	419:434	The behavior of these compounds is significantly affected by pectic polysaccharides, whereby the extent of their influence on red wine quality depends on their structural features and their interactions with the polyphenols.
37188318	4	97	theme	polyphenolic	820:831	arg1	composition					833:843	the polyphenolic composition	816:843	the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts	816:919	This was accomplished by preparation of polysaccharide deprived wines and comparison of the polyphenolic composition of both, the wines and their corresponding polysaccharide-free counterparts.
37188318	3	98	theme	present	525:531	arg1	study					533:537	the present study	521:537	the present study	521:537	In the present study, the composition of the pectic polysaccharides of commercially available Cabernet Sauvignon wines and their impact on anthocyanin, tannin, and polymeric pigment analyses was characterized.
37188318	8	99	theme	pigments	1744:1751	arg1	precipitability					1721:1735	The increased precipitability	1707:1735	The increased precipitability of the pigments due to the interactions with the polysaccharides	1707:1800	The increased precipitability of the pigments due to the interactions with the polysaccharides may indicate the formation of pigmented yet non-covalent aggregates that show comparable properties to the covalently formed precipitable pigments.
35267359	0	0	theme	Physicochemical	81:95	arg1	Properties					97:106	Physicochemical Properties	81:106	Physicochemical Properties	81:106	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	6	1	theme	C.	958:959	arg1	polysaccharides					973:987	C. pyrenoidosa polysaccharides	958:987	C. pyrenoidosa polysaccharides	958:987	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	2	2	theme	physicochemical	396:410	arg1	characteristics					412:426	their physicochemical characteristics	390:426	their physicochemical characteristics	390:426	C. pyrenoidosa polysaccharides were prepared and their physicochemical characteristics were determined.
35267359	4	3	theme	monosaccharide	688:701	arg1	compositions					703:714	monosaccharide compositions	688:714	monosaccharide compositions	688:714	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	3	theme	monosaccharide	688:701	arg1	Rha					729:731	Rha	729:731	Rha	729:731	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	3	theme	monosaccharide	688:701	arg1	GlcA					734:737	GlcA	734:737	GlcA	734:737	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	3	theme	monosaccharide	688:701	arg1	Xyl					750:752	Xyl	750:752	Xyl	750:752	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	3	theme	monosaccharide	688:701	arg1	Ara					758:760	Ara	758:760	Ara	758:760	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	3	theme	monosaccharide	688:701	arg1	Rib					724:726	Rib	724:726	Rib	724:726	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	3	theme	monosaccharide	688:701	arg1	Glc					740:742	Glc	740:742	Glc	740:742	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	3	theme	monosaccharide	688:701	arg1	Gal					745:747	Gal	745:747	Gal	745:747	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	5	4	theme	pyrenoidosa	766:776	arg1	polysaccharides					778:792	C. pyrenoidosa polysaccharides	763:792	C. pyrenoidosa polysaccharides	763:792	C. pyrenoidosa polysaccharides could not be degraded under saliva and the gastrointestinal conditions.
35267359	4	5	theme	pyrenoidosa	597:607	arg1	polysaccharides					609:623	C. pyrenoidosa polysaccharides	594:623	C. pyrenoidosa polysaccharides	594:623	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	9	6	contain	had	1595:1597	arg1	polysaccharides					1579:1593	C. pyrenoidosa polysaccharides	1564:1593	C. pyrenoidosa polysaccharides	1564:1593	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	9	6	contain	had	1595:1597	arg2	functions					1609:1617	prebiotic functions	1599:1617	prebiotic functions	1599:1617	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	9	7	theme	prebiotic	1599:1607	arg1	functions					1609:1617	prebiotic functions	1599:1617	prebiotic functions	1599:1617	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	0	8	dep	In	141:142	arg1	Vitro					144:148	Vitro	144:148	Vitro	144:148	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	9	9	theme	different	1624:1632	arg1	characteristics					1647:1661	different fermentation characteristics	1624:1661	different fermentation characteristics	1624:1661	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	4	10	theme	C.	594:595	arg1	polysaccharides					609:623	C. pyrenoidosa polysaccharides	594:623	C. pyrenoidosa polysaccharides	594:623	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	7	11	theme	harmful	1216:1222	arg1	bacteria					1224:1231	harmful bacteria	1216:1231	harmful bacteria	1216:1231	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	9	12	theme	human	1778:1782	arg1	health					1784:1789	human health	1778:1789	human health	1778:1789	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	4	13	with	fractions	673:681	arg1	compositions					703:714	monosaccharide compositions	688:714	monosaccharide compositions	688:714	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	13	with	fractions	673:681	arg1	Rha					729:731	Rha	729:731	Rha	729:731	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	13	with	fractions	673:681	arg1	GlcA					734:737	GlcA	734:737	GlcA	734:737	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	13	with	fractions	673:681	arg1	Xyl					750:752	Xyl	750:752	Xyl	750:752	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	13	with	fractions	673:681	arg1	Ara					758:760	Ara	758:760	Ara	758:760	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	13	with	fractions	673:681	arg1	Rib					724:726	Rib	724:726	Rib	724:726	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	13	with	fractions	673:681	arg1	Glc					740:742	Glc	740:742	Glc	740:742	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	13	with	fractions	673:681	arg1	Gal					745:747	Gal	745:747	Gal	745:747	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	7	14	theme	pyrenoidosa	1074:1084	arg1	polysaccharides					1086:1100	C. pyrenoidosa polysaccharides	1071:1100	C. pyrenoidosa polysaccharides	1071:1100	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	8	15	theme	prevention	1505:1514	arg1	promotion					1470:1478	the promotion	1466:1478	the promotion of intestinal health and prevention of diseases	1466:1526	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	7	16	theme	bacteria	1224:1231	arg1	growth					1206:1211	growth	1206:1211	growth of harmful bacteria	1206:1231	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	6	17	theme	molecular	879:887	arg1	weight					889:894	the molecular weight	875:894	the molecular weight	875:894	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	9	18	theme	C.	1564:1565	arg1	polysaccharides					1579:1593	C. pyrenoidosa polysaccharides	1564:1593	C. pyrenoidosa polysaccharides	1564:1593	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	5	19	theme	gastrointestinal	837:852	arg1	conditions					854:863	the gastrointestinal conditions	833:863	the gastrointestinal conditions	833:863	C. pyrenoidosa polysaccharides could not be degraded under saliva and the gastrointestinal conditions.
35267359	6	20	theme	polysaccharides	973:987	arg1	carbohydrates					921:933	residual carbohydrates	912:933	residual carbohydrates	912:933	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	6	20	theme	polysaccharides	973:987	arg1	sugars					948:953	reducing sugars	939:953	reducing sugars	939:953	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	7	21	theme	growth	1206:1211	arg1	reduction					1238:1246	reduction	1238:1246	reduction of the ratio of Firmicutes to Bacteroidetes	1238:1290	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	7	21	theme	growth	1206:1211	arg1	promotion					1158:1166	the promotion	1154:1166	the promotion of beneficial bacteria	1154:1189	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	7	21	theme	growth	1206:1211	arg1	inhibition					1192:1201	inhibition	1192:1201	inhibition of growth of harmful bacteria	1192:1231	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	8	22	theme	health	1494:1499	arg1	promotion					1470:1478	the promotion	1466:1478	the promotion of intestinal health and prevention of diseases	1466:1526	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	3	23	theme	C.	499:500	arg1	polysaccharides					514:528	C. pyrenoidosa polysaccharides	499:528	C. pyrenoidosa polysaccharides	499:528	The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models.
35267359	3	24	theme	polysaccharides	514:528	arg1	characteristics					480:494	The digestibility and fermentation characteristics	445:494	characteristics	480:494	The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models.
35267359	0	25	theme	Fermentation	112:123	arg1	Characteristics					125:139	Fermentation Characteristics	112:139	Fermentation Characteristics	112:139	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	6	26	theme	pyrenoidosa	961:971	arg1	polysaccharides					973:987	C. pyrenoidosa polysaccharides	958:987	C. pyrenoidosa polysaccharides	958:987	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	3	27	theme	digestibility	449:461	arg1	characteristics					480:494	The digestibility and fermentation characteristics	445:494	characteristics	480:494	The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models.
35267359	0	28	theme	pyrenoidosa	10:20	arg1	Polysaccharides					22:36	Chlorella pyrenoidosa Polysaccharides	0:36	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.	0:149	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	4	29	theme	polysaccharide	658:671	arg1	fractions					673:681	five non-starch polysaccharide fractions	642:681	five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara	642:760	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	2	30	theme	pyrenoidosa	344:354	arg1	polysaccharides					356:370	C. pyrenoidosa polysaccharides	341:370	C. pyrenoidosa polysaccharides	341:370	C. pyrenoidosa polysaccharides were prepared and their physicochemical characteristics were determined.
35267359	1	31	theme	prebiotic	195:203	arg1	potential					205:213	the prebiotic potential	191:213	the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food	191:338	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	0	32	theme	Chlorella	0:8	arg1	Polysaccharides					22:36	Chlorella pyrenoidosa Polysaccharides	0:36	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.	0:149	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	4	33	theme	non-starch	647:656	arg1	fractions					673:681	five non-starch polysaccharide fractions	642:681	five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara	642:760	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	2	34	theme	C.	341:342	arg1	polysaccharides					356:370	C. pyrenoidosa polysaccharides	341:370	C. pyrenoidosa polysaccharides	341:370	C. pyrenoidosa polysaccharides were prepared and their physicochemical characteristics were determined.
35267359	3	35	theme	pyrenoidosa	502:512	arg1	polysaccharides					514:528	C. pyrenoidosa polysaccharides	499:528	C. pyrenoidosa polysaccharides	499:528	The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models.
35267359	6	36	from	speed	1055:1059	arg1	fermentation					1028:1039	fecal fermentation	1022:1039	fecal fermentation at a moderate speed	1022:1059	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	5	37	theme	C.	763:764	arg1	polysaccharides					778:792	C. pyrenoidosa polysaccharides	763:792	C. pyrenoidosa polysaccharides	763:792	C. pyrenoidosa polysaccharides could not be degraded under saliva and the gastrointestinal conditions.
35267359	9	38	theme	pyrenoidosa	1567:1577	arg1	polysaccharides					1579:1593	C. pyrenoidosa polysaccharides	1564:1593	C. pyrenoidosa polysaccharides	1564:1593	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	8	39	theme	diseases	1519:1526	arg1	prevention					1505:1514	prevention	1505:1514	prevention of diseases	1505:1526	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	8	39	theme	diseases	1519:1526	arg1	health					1494:1499	intestinal health	1483:1499	intestinal health	1483:1499	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	3	40	theme	in	551:552	arg1	models					560:565	in vitro models	551:565	in vitro models	551:565	The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models.
35267359	7	41	theme	Firmicutes	1264:1273	arg1	ratio					1255:1259	the ratio	1251:1259	the ratio of Firmicutes to Bacteroidetes	1251:1290	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	1	42	theme	green	321:325	arg1	food					335:338	a green healthy food	319:338	a green healthy food	319:338	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	1	42	theme	green	321:325	arg1	pyrenoidosa					304:314	C. pyrenoidosa	301:314	C. pyrenoidosa	301:314	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	0	43	theme	In	141:142	arg1	Properties					97:106	Physicochemical Properties	81:106	Physicochemical Properties	81:106	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	1	44	theme	Chlorella	218:226	arg1	polysaccharides					240:254	Chlorella pyrenoidosa polysaccharides	218:254	Chlorella pyrenoidosa polysaccharides	218:254	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	1	45	theme	healthy	327:333	arg1	food					335:338	a green healthy food	319:338	a green healthy food	319:338	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	1	45	theme	healthy	327:333	arg1	pyrenoidosa					304:314	C. pyrenoidosa	301:314	C. pyrenoidosa	301:314	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	6	46	theme	sugars	948:953	arg1	contents					900:907	contents	900:907	contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides	900:987	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	6	46	theme	sugars	948:953	arg1	weight					889:894	the molecular weight	875:894	the molecular weight	875:894	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	8	47	theme	Parabacteroides	1360:1374	arg1	distasonis					1376:1385	Parabacteroides distasonis	1360:1385	Parabacteroides distasonis	1360:1385	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	1	48	theme	pyrenoidosa	228:238	arg1	polysaccharides					240:254	Chlorella pyrenoidosa polysaccharides	218:254	Chlorella pyrenoidosa polysaccharides	218:254	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	7	49	theme	ratio	1255:1259	arg1	reduction					1238:1246	reduction	1238:1246	reduction of the ratio of Firmicutes to Bacteroidetes	1238:1290	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	7	49	theme	ratio	1255:1259	arg1	promotion					1158:1166	the promotion	1154:1166	the promotion of beneficial bacteria	1154:1189	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	7	49	theme	ratio	1255:1259	arg1	inhibition					1192:1201	inhibition	1192:1201	inhibition of growth of harmful bacteria	1192:1231	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	6	50	theme	moderate	1046:1053	arg1	speed					1055:1059	a moderate speed	1044:1059	a moderate speed	1044:1059	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	7	51	theme	beneficial	1171:1180	arg1	bacteria					1182:1189	beneficial bacteria	1171:1189	beneficial bacteria	1171:1189	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	6	52	theme	reducing	939:946	arg1	sugars					948:953	reducing sugars	939:953	reducing sugars	939:953	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	8	53	theme	C.	1307:1308	arg1	polysaccharides					1322:1336	C. pyrenoidosa polysaccharides	1307:1336	C. pyrenoidosa polysaccharides	1307:1336	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	7	54	theme	gut	1128:1130	arg1	microbiota					1132:1141	gut microbiota	1128:1141	gut microbiota	1128:1141	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	1	55	theme	polysaccharides	240:254	arg1	potential					205:213	the prebiotic potential	191:213	the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food	191:338	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	8	56	theme	pyrenoidosa	1310:1320	arg1	polysaccharides					1322:1336	C. pyrenoidosa polysaccharides	1307:1336	C. pyrenoidosa polysaccharides	1307:1336	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	0	57	dep	Prebiotic	43:51	arg1	Modulate					56:63	Modulate	56:63	to Modulate Gut Microbiota	53:78	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	3	58	theme	fermentation	467:478	arg1	characteristics					480:494	The digestibility and fermentation characteristics	445:494	characteristics	480:494	The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models.
35267359	7	59	theme	bacteria	1182:1189	arg1	reduction					1238:1246	reduction	1238:1246	reduction of the ratio of Firmicutes to Bacteroidetes	1238:1290	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	7	59	theme	bacteria	1182:1189	arg1	promotion					1158:1166	the promotion	1154:1166	the promotion of beneficial bacteria	1154:1189	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	7	59	theme	bacteria	1182:1189	arg1	inhibition					1192:1201	inhibition	1192:1201	inhibition of growth of harmful bacteria	1192:1231	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
35267359	6	60	theme	carbohydrates	921:933	arg1	contents					900:907	contents	900:907	contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides	900:987	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	6	60	theme	carbohydrates	921:933	arg1	weight					889:894	the molecular weight	875:894	the molecular weight	875:894	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	8	61	theme	acid	1418:1421	arg1	contents					1423:1430	short-chain fatty acid contents	1400:1430	short-chain fatty acid contents	1400:1430	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	8	62	theme	fatty	1412:1416	arg1	contents					1423:1430	short-chain fatty acid contents	1400:1430	short-chain fatty acid contents	1400:1430	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	6	63	theme	residual	912:919	arg1	carbohydrates					921:933	residual carbohydrates	912:933	residual carbohydrates	912:933	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	8	64	theme	distasonis	1376:1385	arg1	growth					1350:1355	growth	1350:1355	growth of Parabacteroides distasonis	1350:1385	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	9	65	theme	new	1750:1752	arg1	they					1736:1739	they	1736:1739	they	1736:1739	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	9	65	theme	new	1750:1752	arg1	prebiotic					1754:1762	a new prebiotic	1748:1762	a new prebiotic for improving human health	1748:1789	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	8	66	theme	intestinal	1483:1492	arg1	health					1494:1499	intestinal health	1483:1499	intestinal health	1483:1499	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	0	67	dep	Polysaccharides	22:36	arg1	Properties					97:106	Physicochemical Properties	81:106	Physicochemical Properties	81:106	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	0	67	dep	Polysaccharides	22:36	arg1	Characteristics					125:139	Fermentation Characteristics	112:139	Fermentation Characteristics	112:139	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	9	68	theme	conventional	1677:1688	arg1	prebiotics					1690:1699	conventional prebiotics	1677:1699	conventional prebiotics such as fructooligosaccharide	1677:1729	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	9	68	theme	conventional	1677:1688	arg1	fructooligosaccharide					1709:1729	fructooligosaccharide	1709:1729	fructooligosaccharide	1709:1729	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	4	69	theme	Man	719:721	arg1	compositions					703:714	monosaccharide compositions	688:714	monosaccharide compositions	688:714	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	69	theme	Man	719:721	arg1	Rha					729:731	Rha	729:731	Rha	729:731	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	69	theme	Man	719:721	arg1	GlcA					734:737	GlcA	734:737	GlcA	734:737	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	69	theme	Man	719:721	arg1	Xyl					750:752	Xyl	750:752	Xyl	750:752	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	69	theme	Man	719:721	arg1	Ara					758:760	Ara	758:760	Ara	758:760	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	69	theme	Man	719:721	arg1	Rib					724:726	Rib	724:726	Rib	724:726	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	69	theme	Man	719:721	arg1	Glc					740:742	Glc	740:742	Glc	740:742	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	4	69	theme	Man	719:721	arg1	Gal					745:747	Gal	745:747	Gal	745:747	The results revealed that C. pyrenoidosa polysaccharides were composed of five non-starch polysaccharide fractions with monosaccharide compositions of Man, Rib, Rha, GlcA, Glc, Gal, Xyl and Ara.
35267359	9	70	theme	fermentation	1634:1645	arg1	characteristics					1647:1661	different fermentation characteristics	1624:1661	different fermentation characteristics	1624:1661	Thus, these results suggested that C. pyrenoidosa polysaccharides had prebiotic functions with different fermentation characteristics compared with conventional prebiotics such as fructooligosaccharide, and they may be a new prebiotic for improving human health.
35267359	8	71	theme	short-chain	1400:1410	arg1	contents					1423:1430	short-chain fatty acid contents	1400:1430	short-chain fatty acid contents	1400:1430	Intriguingly, C. pyrenoidosa polysaccharides can promote growth of Parabacteroides distasonis and increase short-chain fatty acid contents, thereby probably contributing to the promotion of intestinal health and prevention of diseases.
35267359	3	72	dep	in	551:552	arg1	vitro					554:558	vitro	554:558	vitro	554:558	The digestibility and fermentation characteristics of C. pyrenoidosa polysaccharides were evaluated using in vitro models.
35267359	1	73	theme	useful	267:272	arg1	information					274:284	useful information	267:284	useful information for developing C. pyrenoidosa as a green healthy food	267:338	This study was conducted to investigate the prebiotic potential of Chlorella pyrenoidosa polysaccharides to provide useful information for developing C. pyrenoidosa as a green healthy food.
35267359	0	74	theme	Gut	65:67	arg1	Microbiota					69:78	Gut Microbiota	65:78	Gut Microbiota	65:78	Chlorella pyrenoidosa Polysaccharides as a Prebiotic to Modulate Gut Microbiota: Physicochemical Properties and Fermentation Characteristics In Vitro.
35267359	6	75	theme	fecal	1022:1026	arg1	fermentation					1028:1039	fecal fermentation	1022:1039	fecal fermentation at a moderate speed	1022:1059	However, the molecular weight and contents of residual carbohydrates and reducing sugars of C. pyrenoidosa polysaccharides were significantly reduced after fecal fermentation at a moderate speed.
35267359	7	76	theme	C.	1071:1072	arg1	polysaccharides					1086:1100	C. pyrenoidosa polysaccharides	1071:1100	C. pyrenoidosa polysaccharides	1071:1100	Notably, C. pyrenoidosa polysaccharides could remarkably modulate gut microbiota, including the promotion of beneficial bacteria, inhibition of growth of harmful bacteria, and reduction of the ratio of Firmicutes to Bacteroidetes.
37344645	3	0	theme	stem	334:337	arg1	hyperactivity					344:356	hair stem cell hyperactivity	329:356	hair stem cell hyperactivity	329:356	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	8	1	theme	human	1154:1158	arg1	follicles					1165:1173	human hair follicles	1154:1173	human hair follicles	1154:1173	Osteopontin is overexpressed in human hairy nevi, and it stimulates new growth of human hair follicles.
37344645	7	2	theme	dermal	1046:1051	arg1	melanocytes					1059:1069	dermal nevus melanocytes	1046:1069	dermal nevus melanocytes	1046:1069	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	1	3	theme	proper	157:162	arg1	regeneration1					164:176	proper regeneration1	157:176	proper regeneration1	157:176	Niche signals maintain stem cells in a prolonged quiescence or transiently activate them for proper regeneration1.
37344645	7	4	theme	conditional	907:917	arg1	deletions					919:927	conditional deletions	907:927	conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells,	907:1005	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	3	5	theme	cell	339:342	arg1	hyperactivity					344:356	hair stem cell hyperactivity	329:356	hair stem cell hyperactivity	329:356	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	9	6	theme	regenerative	1288:1299	arg1	capacity					1301:1308	the regenerative capacity	1284:1308	the regenerative capacity of tissue4	1284:1319	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	3	7	theme	Melanocytic	250:260	arg1	nevi					267:270	Melanocytic skin nevi	250:270	Melanocytic skin nevi in human	250:279	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	9	8	theme	tissue	1451:1456	arg1	renewal					1458:1464	tissue renewal	1451:1464	tissue renewal	1451:1464	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	9	9	theme	genotoxic	1247:1255	arg1	stress					1257:1262	genotoxic stress	1247:1262	genotoxic stress	1247:1262	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	9	10	theme	adjacent	1410:1417	arg1	cells					1431:1435	adjacent intact stem cells	1410:1435	adjacent intact stem cells	1410:1435	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	8	11	theme	follicles	1165:1173	arg1	growth					1144:1149	new growth	1140:1149	new growth of human hair follicles	1140:1173	Osteopontin is overexpressed in human hairy nevi, and it stimulates new growth of human hair follicles.
37344645	5	12	theme	Nevus	591:595	arg1	melanocytes					597:607	Nevus melanocytes	591:607	Nevus melanocytes	591:607	Nevus melanocytes activate a distinct secretome, enriched for signalling factors.
37344645	7	13	theme	epithelial	984:993	arg1	cells					1000:1004	epithelial hair cells	984:1004	epithelial hair cells	984:1004	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	5	14	theme	distinct	620:627	arg1	secretome					629:637	a distinct secretome	618:637	a distinct secretome	618:637	Nevus melanocytes activate a distinct secretome, enriched for signalling factors.
37344645	1	15	theme	Niche	64:68	arg1	signals					70:76	Niche signals	64:76	Niche signals	64:76	Niche signals maintain stem cells in a prolonged quiescence or transiently activate them for proper regeneration1.
37344645	9	16	theme	intact	1419:1424	arg1	cells					1431:1435	adjacent intact stem cells	1410:1435	adjacent intact stem cells	1410:1435	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	8	17	theme	hair	1160:1163	arg1	follicles					1165:1173	human hair follicles	1154:1173	human hair follicles	1154:1173	Osteopontin is overexpressed in human hairy nevi, and it stimulates new growth of human hair follicles.
37344645	7	18	theme	cognate	964:970	arg1	receptor					972:979	its cognate receptor	960:979	its cognate receptor on epithelial hair cells	960:1004	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	7	18	theme	cognate	964:970	arg1	CD44					954:957	CD44	954:957	CD44	954:957	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	9	19	theme	stem	1426:1429	arg1	cells					1431:1435	adjacent intact stem cells	1410:1435	adjacent intact stem cells	1410:1435	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	4	20	theme	stem	480:483	arg1	cells					485:489	epithelial hair stem cells	464:489	epithelial hair stem cells	464:489	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	9	21	theme	senescent	1207:1215	arg1	cells					1217:1221	senescent cells	1207:1221	senescent cells	1207:1221	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	9	22	theme	cells	1431:1435	arg1	activity					1398:1405	the activity	1394:1405	the activity of adjacent intact stem cells	1394:1435	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	10	23	theme	regenerative	1566:1577	arg1	disorders					1579:1587	regenerative disorders	1566:1587	regenerative disorders	1566:1587	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	9	24	theme	cells	1217:1221	arg1	accumulation					1191:1202	broad accumulation	1185:1202	broad accumulation of senescent cells, such as upon ageing or genotoxic stress,	1185:1263	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	1	25	theme	stem	87:90	arg1	cells					92:96	stem cells	87:96	stem cells	87:96	Niche signals maintain stem cells in a prolonged quiescence or transiently activate them for proper regeneration1.
37344645	10	26	theme	attractive	1533:1542	arg1	target					1556:1561	an attractive therapeutic target	1530:1561	an attractive therapeutic target in regenerative disorders	1530:1587	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	10	26	theme	attractive	1533:1542	arg1	cells					1501:1505	senescent cells	1491:1505	senescent cells	1491:1505	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	10	26	theme	attractive	1533:1542	arg1	secretome					1517:1525	their secretome	1511:1525	their secretome	1511:1525	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	3	27	theme	skin	262:265	arg1	nevi					267:270	Melanocytic skin nevi	250:270	Melanocytic skin nevi in human	250:279	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	4	28	theme	mouse	379:383	arg1	models					385:390	genetic mouse models	371:390	genetic mouse models of nevi2,3	371:401	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	8	29	theme	human	1104:1108	arg1	nevi					1116:1119	human hairy nevi	1104:1119	human hairy nevi	1104:1119	Osteopontin is overexpressed in human hairy nevi, and it stimulates new growth of human hair follicles.
37344645	10	30	theme	senescent	1491:1499	arg1	cells					1501:1505	senescent cells	1491:1505	senescent cells	1491:1505	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	10	30	theme	senescent	1491:1499	arg1	target					1556:1561	an attractive therapeutic target	1530:1561	an attractive therapeutic target in regenerative disorders	1530:1587	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	10	30	theme	senescent	1491:1499	arg1	secretome					1517:1525	their secretome	1511:1525	their secretome	1511:1525	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	4	31	theme	nevi2,3	395:401	arg1	models					385:390	genetic mouse models	371:390	genetic mouse models of nevi2,3	371:401	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	7	32	theme	robust	858:863	arg1	growth					870:875	robust hair growth	858:875	robust hair growth	858:875	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	4	33	theme	melanocytes	446:456	arg1	clusters					424:431	dermal clusters	417:431	dermal clusters of senescent melanocytes	417:456	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	3	34	from	nevi	267:270	arg1	human					275:279	human	275:279	human	275:279	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	3	35	theme	excessive	295:303	arg1	growth					310:315	excessive hair growth	295:315	excessive hair growth	295:315	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	7	36	theme	CD44	954:957	arg1	germline					894:901	germline	894:901	germline	894:901	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	7	36	theme	CD44	954:957	arg1	deletions					919:927	conditional deletions	907:927	conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells,	907:1005	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	6	37	theme	signalling	704:713	arg1	Osteopontin					673:683	Osteopontin	673:683	Osteopontin	673:683	Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth.
37344645	6	37	theme	signalling	704:713	arg1	factor					715:720	the leading nevus signalling factor	686:720	the leading nevus signalling factor	686:720	Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth.
37344645	0	38	theme	senescent	14:22	arg1	melanocytes					24:34	senescent melanocytes	14:34	senescent melanocytes	14:34	Signalling by senescent melanocytes hyperactivates hair growth.
37344645	7	39	theme	genetic	811:817	arg1	overexpression					819:832	its genetic overexpression	807:832	its genetic overexpression	807:832	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	3	40	theme	hair	305:308	arg1	growth					310:315	excessive hair growth	295:315	excessive hair growth	295:315	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	2	41	theme	niche	197:201	arg1	signalling					203:212	balanced niche signalling	188:212	balanced niche signalling	188:212	Altering balanced niche signalling can lead to regenerative disorders.
37344645	1	42	theme	prolonged	103:111	arg1	quiescence					113:122	a prolonged quiescence	101:122	a prolonged quiescence	101:122	Niche signals maintain stem cells in a prolonged quiescence or transiently activate them for proper regeneration1.
37344645	6	43	theme	nevus	698:702	arg1	Osteopontin					673:683	Osteopontin	673:683	Osteopontin	673:683	Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth.
37344645	6	43	theme	nevus	698:702	arg1	factor					715:720	the leading nevus signalling factor	686:720	the leading nevus signalling factor	686:720	Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth.
37344645	0	44	theme	hair	51:54	arg1	growth					56:61	hair growth	51:61	hair growth	51:61	Signalling by senescent melanocytes hyperactivates hair growth.
37344645	4	45	theme	hair	577:580	arg1	renewal					582:588	hair renewal	577:588	hair renewal	577:588	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	9	46	theme	tissue4	1313:1319	arg1	capacity					1301:1308	the regenerative capacity	1284:1308	the regenerative capacity of tissue4	1284:1319	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	2	47	theme	balanced	188:195	arg1	signalling					203:212	balanced niche signalling	188:212	balanced niche signalling	188:212	Altering balanced niche signalling can lead to regenerative disorders.
37344645	6	48	theme	leading	690:696	arg1	Osteopontin					673:683	Osteopontin	673:683	Osteopontin	673:683	Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth.
37344645	6	48	theme	leading	690:696	arg1	factor					715:720	the leading nevus signalling factor	686:720	the leading nevus signalling factor	686:720	Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth.
37344645	5	49	theme	signalling	653:662	arg1	factors					664:670	signalling factors	653:670	signalling factors	653:670	Nevus melanocytes activate a distinct secretome, enriched for signalling factors.
37344645	9	50	theme	senescent	1349:1357	arg1	clusters					1364:1371	senescent cell clusters	1349:1371	senescent cell clusters	1349:1371	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	8	51	theme	hairy	1110:1114	arg1	nevi					1116:1119	human hairy nevi	1104:1119	human hairy nevi	1104:1119	Osteopontin is overexpressed in human hairy nevi, and it stimulates new growth of human hair follicles.
37344645	4	52	theme	hair	475:478	arg1	cells					485:489	epithelial hair stem cells	464:489	epithelial hair stem cells	464:489	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	9	53	theme	cell	1359:1362	arg1	clusters					1364:1371	senescent cell clusters	1349:1371	senescent cell clusters	1349:1371	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	7	54	theme	nevus	1053:1057	arg1	melanocytes					1059:1069	dermal nevus melanocytes	1046:1069	dermal nevus melanocytes	1046:1069	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	4	55	theme	epithelial	464:473	arg1	cells					485:489	epithelial hair stem cells	464:489	epithelial hair stem cells	464:489	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	7	56	theme	enhanced	1014:1021	arg1	growth					1028:1033	enhanced hair growth	1014:1033	enhanced hair growth induced by dermal nevus melanocytes	1014:1069	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	9	57	theme	broad	1185:1189	arg1	accumulation					1191:1202	broad accumulation	1185:1202	broad accumulation of senescent cells, such as upon ageing or genotoxic stress,	1185:1263	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37344645	3	58	theme	hair	329:332	arg1	hyperactivity					344:356	hair stem cell hyperactivity	329:356	hair stem cell hyperactivity	329:356	Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity.
37344645	10	59	theme	therapeutic	1544:1554	arg1	target					1556:1561	an attractive therapeutic target	1530:1561	an attractive therapeutic target in regenerative disorders	1530:1587	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	10	59	theme	therapeutic	1544:1554	arg1	cells					1501:1505	senescent cells	1491:1505	senescent cells	1491:1505	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	10	59	theme	therapeutic	1544:1554	arg1	secretome					1517:1525	their secretome	1511:1525	their secretome	1511:1525	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	2	60	theme	regenerative	226:237	arg1	disorders					239:247	regenerative disorders	226:247	regenerative disorders	226:247	Altering balanced niche signalling can lead to regenerative disorders.
37344645	4	61	theme	genetic	371:377	arg1	models					385:390	genetic mouse models	371:390	genetic mouse models of nevi2,3	371:401	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	7	62	theme	osteopontin	792:802	arg1	overexpression					819:832	its genetic overexpression	807:832	its genetic overexpression	807:832	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	7	62	theme	osteopontin	792:802	arg1	Injection					779:787	Injection	779:787	Injection of osteopontin	779:802	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	4	63	theme	dermal	417:422	arg1	clusters					424:431	dermal clusters	417:431	dermal clusters of senescent melanocytes	417:456	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	6	64	theme	hair	766:769	arg1	growth					771:776	hair growth	766:776	hair growth	766:776	Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth.
37344645	7	65	theme	osteopontin	939:949	arg1	germline					894:901	germline	894:901	germline	894:901	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	7	65	theme	osteopontin	939:949	arg1	deletions					919:927	conditional deletions	907:927	conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells,	907:1005	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	7	66	theme	hair	1023:1026	arg1	growth					1028:1033	enhanced hair growth	1014:1033	enhanced hair growth induced by dermal nevus melanocytes	1014:1069	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	4	67	theme	senescent	436:444	arg1	melanocytes					446:456	senescent melanocytes	436:456	senescent melanocytes	436:456	Here, using genetic mouse models of nevi2,3, we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal.
37344645	7	68	theme	hair	865:868	arg1	growth					870:875	robust hair growth	858:875	robust hair growth	858:875	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	10	69	from	target	1556:1561	arg1	disorders					1579:1587	regenerative disorders	1566:1587	regenerative disorders	1566:1587	This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders.
37344645	8	70	theme	new	1140:1142	arg1	growth					1144:1149	new growth	1140:1149	new growth of human hair follicles	1140:1173	Osteopontin is overexpressed in human hairy nevi, and it stimulates new growth of human hair follicles.
37344645	7	71	theme	hair	995:998	arg1	cells					1000:1004	epithelial hair cells	984:1004	epithelial hair cells	984:1004	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	7	72	from	receptor	972:979	arg1	cells					1000:1004	epithelial hair cells	984:1004	epithelial hair cells	984:1004	Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes.
37344645	9	73	dep	such	1224:1227	arg1	as					1229:1230	as	1229:1230	as	1229:1230	Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4, we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal.
37400624	11	0	theme	NRCG4	1639:1643	arg1	Mw					1645:1646	NRCG4 Mw	1639:1646	NRCG4 Mw	1639:1646	NRCG4 Mw was determined to be 4.25 × 105 gmol-1 and the Mn to be 1.97 × 105 gmol-1.
37400624	16	1	theme	disease	2346:2352	arg1	efficacy					2354:2361	The anti-Alzheimer's disease efficacy	2325:2361	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide	2325:2388	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition.
37400624	9	2	theme	liquid	1449:1454	arg1	HPLC					1472:1475	HPLC	1472:1475	HPLC	1472:1475	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	2	theme	liquid	1449:1454	arg1	chromatography					1456:1469	high-performance liquid chromatography	1432:1469	high-performance liquid chromatography (HPLC)	1432:1476	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	11	3	theme	4.25 × 105	1669:1678	arg1	gmol-1					1680:1685	4.25 × 105 gmol-1	1669:1685	4.25 × 105 gmol-1	1669:1685	NRCG4 Mw was determined to be 4.25 × 105 gmol-1 and the Mn to be 1.97 × 105 gmol-1.
37400624	6	4	theme	Streptomyces	839:850	arg1	strain					852:857	an isolated Streptomyces strain	827:857	an isolated Streptomyces strain	827:857	Properties of exopolysaccharide (EPS) produced by an isolated Streptomyces strain were investigated for its capability to play as anti-Alzheimer.
37400624	15	5	theme	anti-tyrosinase	2270:2284	arg1	characteristics					2308:2322	its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics	2204:2322	its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics	2204:2322	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	16	6	theme	exopolysaccharide	2372:2388	arg1	efficacy					2354:2361	The anti-Alzheimer's disease efficacy	2325:2361	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide	2325:2388	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition.
37400624	5	7	from	Sea	600:602	arg1	isolation					569:577	the isolation	565:577	the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs	565:774	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	8	8	with	NRCG4	1082:1086	arg1	number					1103:1108	accession number MK850242	1093:1117	accession number MK850242	1093:1117	NRCG4 with accession number MK850242.
37400624	13	9	theme	various	1891:1897	arg1	methods					1899:1905	various methods	1891:1905	various methods	1891:1905	In addition, antioxidant and anti-inflammation activity was measured through various methods.
37400624	12	10	theme	uronic	1748:1753	arg1	acid					1755:1758	uronic acid	1748:1758	uronic acid (16.0%)	1748:1766	Also, the NRCG4 included uronic acid (16.0%) and sulfate (0.0%), but no protein was found.
37400624	12	10	theme	uronic	1748:1753	arg1	%					1765:1765	16.0%	1761:1765	16.0%	1761:1765	Also, the NRCG4 included uronic acid (16.0%) and sulfate (0.0%), but no protein was found.
37400624	5	11	theme	side	744:747	arg1	effects					749:755	side effects	744:755	side effects of synthetic drugs	744:774	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	4	12	theme	drug	509:512	arg1	discovery					514:522	new drug discovery	505:522	new drug discovery	505:522	Polysaccharides from marine microorganisms are of interest to new drug discovery.
37400624	15	13	theme	anti-inflammatory	2290:2306	arg1	characteristics					2308:2322	its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics	2204:2322	its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics	2204:2322	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	1	14	dep	NRCG4	87:91	arg1	targets					135:141	in-vitro targets	126:141	NRCG4 and its anti-Alzheimer efficacy: in-vitro targets.	87:142	NRCG4 and its anti-Alzheimer efficacy: in-vitro targets.
37400624	10	15	theme	1.2:1.5:2.8:1.0	1608:1622	arg1	ratio					1599:1603	a molar ratio	1591:1603	a molar ratio of 1.2:1.5:2.8:1.0, respectively	1591:1636	The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively.
37400624	15	16	theme	antioxidant	2208:2218	arg1	characteristics					2308:2322	its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics	2204:2322	its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics	2204:2322	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	13	17	theme	anti-inflammation	1843:1859	arg1	activity					1861:1868	antioxidant and anti-inflammation activity	1827:1868	antioxidant and anti-inflammation activity	1827:1868	In addition, antioxidant and anti-inflammation activity was measured through various methods.
37400624	2	18	theme	compositional	280:292	arg1	complexity					294:303	their structural and compositional complexity	259:303	their structural and compositional complexity	259:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	7	19	theme	rRNA	1048:1051	arg1	analysis					1053:1060	rRNA analysis	1048:1060	rRNA analysis	1048:1060	This strain was identified morphologically, physiologically, and biochemically and actually was confirmed by molecularly 16S rRNA analysis as Streptomyces sp.
37400624	2	20	theme	structural	265:274	arg1	complexity					294:303	their structural and compositional complexity	259:303	their structural and compositional complexity	259:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	5	21	theme	Red	596:598	arg1	Egypt					605:609	Egypt	605:609	Egypt	605:609	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	5	21	theme	Red	596:598	arg1	Sea					600:602	Red Sea	596:602	Red Sea	596:602	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	15	22	theme	risk	2183:2186	arg1	factors					2188:2194	Alzheimer's disease risk factors	2163:2194	Alzheimer's disease risk factors	2163:2194	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	3	23	theme	novel	368:372	arg1	functions					374:382	novel functions	368:382	novel functions	368:382	Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions.
37400624	2	24	theme	powerful	188:195	arg1	molecules					197:205	extremely powerful molecules	178:205	extremely powerful molecules	178:205	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	2	24	theme	powerful	188:195	arg1	Exopolysaccharides					155:172	BACKGROUND Exopolysaccharides	144:172	BACKGROUND Exopolysaccharides	144:172	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	5	25	dep	RESULTS	525:531	arg1	focused					554:560	focused	554:560	focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs	554:774	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	9	26	theme	produced	1124:1131	arg1	EPS					1133:1135	The produced EPS	1120:1135	The produced EPS	1120:1135	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	5	27	theme	drugs	770:774	arg1	effects					749:755	side effects	744:755	side effects of synthetic drugs	744:774	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	8	28	theme	accession	1093:1101	arg1	number					1103:1108	accession number MK850242	1093:1117	accession number MK850242	1093:1117	NRCG4 with accession number MK850242.
37400624	5	29	theme	new	647:649	arg1	exopolysaccharide					659:675	a new natural exopolysaccharide	645:675	a new natural exopolysaccharide	645:675	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	14	30	theme	abilities	2087:2095	arg1	inhibition					1998:2007	inhibition	1998:2007	inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities	1998:2095	This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer's characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities.
37400624	9	31	theme	high-performance	1372:1387	arg1	HPGPC					1420:1424	HPGPC	1420:1424	HPGPC	1420:1424	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	31	theme	high-performance	1372:1387	arg1	chromatography					1404:1417	high-performance gel permeation chromatography	1372:1417	high-performance gel permeation chromatography (HPGPC)	1372:1425	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	1	32	theme	anti-Alzheimer	101:114	arg1	efficacy					116:123	its anti-Alzheimer efficacy	97:123	its anti-Alzheimer efficacy	97:123	NRCG4 and its anti-Alzheimer efficacy: in-vitro targets.
37400624	3	33	theme	living	424:429	arg1	conditions					431:440	their special living conditions	410:440	their special living conditions	410:440	Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions.
37400624	9	34	theme	1:4	1171:1173	arg1	volumes					1175:1181	precipitation 1:4 volumes	1157:1181	precipitation 1:4 volumes of chilled ethanol	1157:1200	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	15	35	theme	radical	2238:2244	arg1	scavenging					2246:2255	radical scavenging	2238:2255	radical scavenging capability	2238:2266	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	0	36	theme	exopolysaccharide	40:56	arg1	Characterization					0:15	Characterization	0:15	Characterization of biologically active exopolysaccharide	0:56	Characterization of biologically active exopolysaccharide produced by Streptomyces sp.
37400624	9	37	theme	gel	1389:1391	arg1	HPGPC					1420:1424	HPGPC	1420:1424	HPGPC	1420:1424	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	37	theme	gel	1389:1391	arg1	chromatography					1404:1417	high-performance gel permeation chromatography	1372:1417	high-performance gel permeation chromatography (HPGPC)	1372:1425	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	13	38	theme	antioxidant	1827:1837	arg1	activity					1861:1868	antioxidant and anti-inflammation activity	1827:1868	antioxidant and anti-inflammation activity	1827:1868	In addition, antioxidant and anti-inflammation activity was measured through various methods.
37400624	9	39	theme	ethanol	1194:1200	arg1	fraction					1222:1229	the third major fraction	1206:1229	the third major fraction (1:3)	1206:1235	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	39	theme	ethanol	1194:1200	arg1	1:3					1232:1234	1:3	1232:1234	1:3	1232:1234	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	39	theme	ethanol	1194:1200	arg1	volumes					1175:1181	precipitation 1:4 volumes	1157:1181	precipitation 1:4 volumes of chilled ethanol	1157:1200	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	2	40	with	molecules	197:205	arg1	range					219:223	a wide range	212:223	a wide range of uses in pharmaceuticals due to their structural and compositional complexity	212:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	2	41	theme	uses	228:231	arg1	range					219:223	a wide range	212:223	a wide range of uses in pharmaceuticals due to their structural and compositional complexity	212:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	11	42	theme	1.97 × 105	1704:1713	arg1	gmol-1					1715:1720	1.97 × 105 gmol-1	1704:1720	1.97 × 105 gmol-1	1704:1720	NRCG4 Mw was determined to be 4.25 × 105 gmol-1 and the Mn to be 1.97 × 105 gmol-1.
37400624	9	43	theme	major	1216:1220	arg1	1:3					1232:1234	1:3	1232:1234	1:3	1232:1234	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	43	theme	major	1216:1220	arg1	fraction					1222:1229	the third major fraction	1206:1229	the third major fraction (1:3)	1206:1235	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	44	theme	high-performance	1432:1447	arg1	HPLC					1472:1475	HPLC	1472:1475	HPLC	1472:1475	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	44	theme	high-performance	1432:1447	arg1	chromatography					1456:1469	high-performance liquid chromatography	1432:1469	high-performance liquid chromatography (HPLC)	1432:1476	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	4	45	theme	marine	464:469	arg1	microorganisms					471:484	marine microorganisms	464:484	marine microorganisms	464:484	Polysaccharides from marine microorganisms are of interest to new drug discovery.
37400624	2	46	from	range	219:223	arg1	pharmaceuticals					236:250	pharmaceuticals	236:250	pharmaceuticals due to their structural and compositional complexity	236:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	4	47	from	microorganisms	471:484	arg1	Polysaccharides					443:457	Polysaccharides	443:457	Polysaccharides from marine microorganisms	443:484	Polysaccharides from marine microorganisms are of interest to new drug discovery.
37400624	6	48	theme	isolated	830:837	arg1	strain					852:857	an isolated Streptomyces strain	827:857	an isolated Streptomyces strain	827:857	Properties of exopolysaccharide (EPS) produced by an isolated Streptomyces strain were investigated for its capability to play as anti-Alzheimer.
37400624	15	49	dep	antioxidant	2208:2218	arg1	chelation					2227:2235	metal chelation	2221:2235	metal chelation	2221:2235	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	15	49	dep	antioxidant	2208:2218	arg1	capability					2257:2266	radical scavenging capability	2238:2266	radical scavenging capability	2238:2266	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	17	50	theme	present	2469:2475	arg1	study					2477:2481	The present study	2465:2481	The present study highlighted those exopolysaccharides	2465:2518	CONCLUSIONS The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
37400624	16	51	theme	NRCG4	2366:2370	arg1	exopolysaccharide					2372:2388	NRCG4 exopolysaccharide	2366:2388	NRCG4 exopolysaccharide	2366:2388	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition.
37400624	14	52	theme	cholinesterase	2012:2025	arg1	inhibition					1998:2007	inhibition	1998:2007	inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities	1998:2095	This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer's characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities.
37400624	10	53	theme	molar	1593:1597	arg1	ratio					1599:1603	a molar ratio	1591:1603	a molar ratio of 1.2:1.5:2.8:1.0, respectively	1591:1636	The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively.
37400624	17	54	dep	industry	2565:2572	arg1	anti-inflammatory					2608:2624	anti-inflammatory	2608:2624	anti-inflammatory	2608:2624	CONCLUSIONS The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
37400624	17	54	dep	industry	2565:2572	arg1	agents					2643:2648	antioxidant agents	2631:2648	antioxidant agents	2631:2648	CONCLUSIONS The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
37400624	17	54	dep	industry	2565:2572	arg1	anti-tyrosinase					2591:2605	anti-tyrosinase	2591:2605	anti-tyrosinase	2591:2605	CONCLUSIONS The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
37400624	17	54	dep	industry	2565:2572	arg1	anti-Alzheimer					2575:2588	anti-Alzheimer	2575:2588	anti-Alzheimer	2575:2588	CONCLUSIONS The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
37400624	5	55	theme	current	537:543	arg1	research					545:552	The current research	533:552	The current research	533:552	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	15	56	theme	potential	2126:2134	arg1	role					2136:2139	a potential role	2124:2139	a potential role	2124:2139	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	16	57	theme	unique	2413:2418	arg1	composition					2440:2450	its unique determined chemical composition	2409:2450	its unique determined chemical composition	2409:2450	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition.
37400624	9	58	theme	functional	1267:1276	arg1	groups					1278:1283	the functional groups	1263:1283	the functional groups	1263:1283	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	3	59	theme	Marine	306:311	arg1	microorganisms					313:326	Marine microorganisms	306:326	Marine microorganisms	306:326	Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions.
37400624	5	60	contain	have	617:620	arg1	Egypt					605:609	Egypt	605:609	Egypt	605:609	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	5	60	contain	have	617:620	arg1	Sea					600:602	Red Sea	596:602	Red Sea	596:602	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	5	60	contain	have	617:620	arg2	ability					626:632	the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs	622:774	the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs	622:774	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	2	61	theme	due	252:254	arg1	pharmaceuticals					236:250	pharmaceuticals	236:250	pharmaceuticals due to their structural and compositional complexity	236:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	1	62	theme	in-vitro	126:133	arg1	targets					135:141	in-vitro targets	126:141	NRCG4 and its anti-Alzheimer efficacy: in-vitro targets.	87:142	NRCG4 and its anti-Alzheimer efficacy: in-vitro targets.
37400624	7	63	theme	Streptomyces	1065:1076	arg1	sp					1078:1079	Streptomyces sp	1065:1079	Streptomyces sp	1065:1079	This strain was identified morphologically, physiologically, and biochemically and actually was confirmed by molecularly 16S rRNA analysis as Streptomyces sp.
37400624	15	64	theme	factors	2188:2194	arg1	suppression					2148:2158	the suppression	2144:2158	the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics	2144:2322	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	3	65	theme	bioactive	342:350	arg1	substances					352:361	bioactive substances	342:361	bioactive substances with novel functions and structures	342:397	Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions.
37400624	4	66	theme	new	505:507	arg1	discovery					514:522	new drug discovery	505:522	new drug discovery	505:522	Polysaccharides from marine microorganisms are of interest to new drug discovery.
37400624	9	67	theme	chemical	1294:1301	arg1	evaluation					1303:1312	chemical evaluation	1294:1312	chemical evaluation	1294:1312	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	15	68	theme	disease	2175:2181	arg1	factors					2188:2194	Alzheimer's disease risk factors	2163:2194	Alzheimer's disease risk factors	2163:2194	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	10	69	from	EPS	1524:1526	arg1	ratio					1599:1603	a molar ratio	1591:1603	a molar ratio of 1.2:1.5:2.8:1.0, respectively	1591:1636	The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively.
37400624	5	70	theme	synthetic	760:768	arg1	drugs					770:774	synthetic drugs	760:774	synthetic drugs	760:774	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	16	71	theme	chemical	2431:2438	arg1	composition					2440:2450	its unique determined chemical composition	2409:2450	its unique determined chemical composition	2409:2450	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition.
37400624	3	72	theme	special	416:422	arg1	conditions					431:440	their special living conditions	410:440	their special living conditions	410:440	Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions.
37400624	9	73	theme	Fourier-transform	1337:1353	arg1	FTIR					1365:1368	FTIR	1365:1368	FTIR	1365:1368	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	73	theme	Fourier-transform	1337:1353	arg1	infrared					1355:1362	Fourier-transform infrared	1337:1362	Fourier-transform infrared (FTIR)	1337:1369	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	5	74	theme	natural	651:657	arg1	exopolysaccharide					659:675	a new natural exopolysaccharide	645:675	a new natural exopolysaccharide	645:675	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
37400624	2	75	from	pharmaceuticals	236:250	arg1	range					219:223	a wide range	212:223	a wide range of uses in pharmaceuticals due to their structural and compositional complexity	212:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	14	76	theme	antioxidant	2075:2085	arg1	abilities					2087:2095	anti-inflammatory and antioxidant abilities	2053:2095	cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities	2012:2095	This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer's characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities.
37400624	3	77	with	substances	352:361	arg1	structures					388:397	structures	388:397	structures	388:397	Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions.
37400624	3	77	with	substances	352:361	arg1	functions					374:382	novel functions	368:382	novel functions	368:382	Marine microorganisms often produce bioactive substances with novel functions and structures because of their special living conditions.
37400624	14	78	theme	anti-inflammatory	2053:2069	arg1	abilities					2087:2095	anti-inflammatory and antioxidant abilities	2053:2095	cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities	2012:2095	This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer's characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities.
37400624	17	79	theme	antioxidant	2631:2641	arg1	agents					2643:2648	antioxidant agents	2631:2648	antioxidant agents	2631:2648	CONCLUSIONS The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
37400624	17	80	dep	CONCLUSIONS	2453:2463	arg1	harnessed					2529:2537	harnessed	2529:2537	could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents)	2520:2649	CONCLUSIONS The present study highlighted those exopolysaccharides could be harnessed to improve pharmaceutical industry (anti-Alzheimer, anti-tyrosinase, anti-inflammatory, and antioxidant agents).
37400624	9	81	theme	precipitation	1157:1169	arg1	volumes					1175:1181	precipitation 1:4 volumes	1157:1181	precipitation 1:4 volumes of chilled ethanol	1157:1200	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	0	82	theme	active	33:38	arg1	exopolysaccharide					40:56	biologically active exopolysaccharide	20:56	biologically active exopolysaccharide	20:56	Characterization of biologically active exopolysaccharide produced by Streptomyces sp.
37400624	14	83	theme	NRCG4	1934:1938	arg1	exopolysaccharide					1940:1956	NRCG4 exopolysaccharide	1934:1956	NRCG4 exopolysaccharide	1934:1956	This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer's characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities.
37400624	16	84	theme	determined	2420:2429	arg1	composition					2440:2450	its unique determined chemical composition	2409:2450	its unique determined chemical composition	2409:2450	The anti-Alzheimer's disease efficacy of NRCG4 exopolysaccharide may be assigned to its unique determined chemical composition.
37400624	10	85	theme	acidic	1517:1522	arg1	EPS					1524:1526	an acidic EPS	1514:1526	an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose	1514:1586	The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively.
37400624	10	85	theme	acidic	1517:1522	arg1	NRCG4					1504:1508	NRCG4	1504:1508	NRCG4	1504:1508	The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively.
37400624	2	86	theme	wide	214:217	arg1	range					219:223	a wide range	212:223	a wide range of uses in pharmaceuticals due to their structural and compositional complexity	212:303	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	15	87	theme	scavenging	2246:2255	arg1	chelation					2227:2235	metal chelation	2221:2235	metal chelation	2221:2235	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	15	87	theme	scavenging	2246:2255	arg1	capability					2257:2266	radical scavenging capability	2238:2266	radical scavenging capability	2238:2266	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	14	88	theme	tyrosinase	2031:2040	arg1	inhibition					1998:2007	inhibition	1998:2007	inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities	1998:2095	This study confirmed that NRCG4 exopolysaccharide exerted anti-Alzheimer's characters via inhibition of cholinesterase and tyrosinase as well as anti-inflammatory and antioxidant abilities.
37400624	9	89	theme	chilled	1186:1192	arg1	ethanol					1194:1200	chilled ethanol	1186:1200	chilled ethanol	1186:1200	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	0	90	theme	Streptomyces	70:81	arg1	sp					83:84	Streptomyces sp	70:84	Streptomyces sp	70:84	Characterization of biologically active exopolysaccharide produced by Streptomyces sp.
37400624	9	91	theme	permeation	1393:1402	arg1	HPGPC					1420:1424	HPGPC	1420:1424	HPGPC	1420:1424	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	91	theme	permeation	1393:1402	arg1	chromatography					1404:1417	high-performance gel permeation chromatography	1372:1417	high-performance gel permeation chromatography (HPGPC)	1372:1425	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	10	92	theme	mannuronic	1540:1549	arg1	acid					1551:1554	mannuronic acid	1540:1554	mannuronic acid	1540:1554	The findings showed that NRCG4 was an acidic EPS composed of mannuronic acid, glucose, mannose, and rhamnose in a molar ratio of 1.2:1.5:2.8:1.0, respectively.
37400624	6	93	theme	exopolysaccharide	791:807	arg1	Properties					777:786	Properties	777:786	Properties of exopolysaccharide (EPS) produced by an isolated Streptomyces strain	777:857	Properties of exopolysaccharide (EPS) produced by an isolated Streptomyces strain were investigated for its capability to play as anti-Alzheimer.
37400624	15	94	theme	metal	2221:2225	arg1	chelation					2227:2235	metal chelation	2221:2235	metal chelation	2221:2235	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	15	94	theme	metal	2221:2225	arg1	capability					2257:2266	radical scavenging capability	2238:2266	radical scavenging capability	2238:2266	Additionally, it occurred a potential role in the suppression of Alzheimer's disease risk factors through its antioxidant (metal chelation, radical scavenging capability), anti-tyrosinase and anti-inflammatory characteristics.
37400624	9	95	theme	third	1210:1214	arg1	1:3					1232:1234	1:3	1232:1234	1:3	1232:1234	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	9	95	theme	third	1210:1214	arg1	fraction					1222:1229	the third major fraction	1206:1229	the third major fraction (1:3)	1206:1235	The produced EPS was fractionated by precipitation 1:4 volumes of chilled ethanol and the third major fraction (1:3) listed as NRCG4, and then the functional groups, MW, and chemical evaluation have been detected via Fourier-transform infrared (FTIR), high-performance gel permeation chromatography (HPGPC), and high-performance liquid chromatography (HPLC).
37400624	2	96	theme	BACKGROUND	144:153	arg1	molecules					197:205	extremely powerful molecules	178:205	extremely powerful molecules	178:205	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	2	96	theme	BACKGROUND	144:153	arg1	Exopolysaccharides					155:172	BACKGROUND Exopolysaccharides	144:172	BACKGROUND Exopolysaccharides	144:172	BACKGROUND Exopolysaccharides are extremely powerful molecules with a wide range of uses in pharmaceuticals due to their structural and compositional complexity.
37400624	5	97	theme	bacteria	582:589	arg1	isolation					569:577	the isolation	565:577	the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs	565:774	RESULTS The current research focused on the isolation of bacteria from Red Sea, Egypt, that have the ability to produce a new natural exopolysaccharide in order to be examined in treating Alzheimer's illness to obviate side effects of synthetic drugs.
35821704	7	0	theme	vein	1192:1195	arg1	thrombosis					1197:1206	tail vein thrombosis	1187:1206	tail vein thrombosis	1187:1206	Pathological observations showed that LDSB-KSFY07 reduced liver tissue lesions and tail vein thrombosis.
35821704	10	1	theme	mice	1833:1836	arg1	expression					1768:1777	tissue plasminogen activator (t-PA) expression	1732:1777	tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice	1732:1836	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	10	2	theme	LDSB-KSFY07	1639:1649	arg1	Meanwhile					1628:1636	Meanwhile	1628:1636	Meanwhile	1628:1636	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	4	3	theme	intestinal	422:431	arg1	composition					443:453	intestinal microbial composition	422:453	intestinal microbial composition	422:453	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	2	4	theme	thrombosis	168:177	arg1	model					179:183	A mouse thrombosis model	160:183	A mouse thrombosis model	160:183	A mouse thrombosis model was established by kappa-carrageenan to observe the inhibitory effect of Lactobacillus delbrueckii subsp.
35821704	5	5	theme	tail	709:712	arg1	degree					693:698	the degree	689:698	the degree of black tail	689:712	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	11	6	theme	microbial	1963:1971	arg1	expression					1973:1982	Bacteroides, Lactobacterium, and Bifidobacterium microbial expression	1914:1982	expression	1973:1982	A mouse faeces examination revealed that LDSB-KSFY07 could also upregulate Bacteroides, Lactobacterium, and Bifidobacterium microbial expression and downregulate Firmicutes expression in the gut.
35821704	9	7	from	expression	1465:1474	arg1	tissue					1620:1625	tail vein vascular tissue	1601:1625	tail vein vascular tissue	1601:1625	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	10	8	theme	activator	1675:1683	arg1	PAI-1					1698:1702	PAI-1	1698:1702	PAI-1	1698:1702	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	10	8	theme	activator	1675:1683	arg1	inhibitor-1					1685:1695	plasminogen activator inhibitor-1	1663:1695	plasminogen activator inhibitor-1 (PAI-1) mRNA expression	1663:1719	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	9	9	theme	vein	1606:1609	arg1	tissue					1620:1625	tail vein vascular tissue	1601:1625	tail vein vascular tissue	1601:1625	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	4	10	theme	tissue-related	391:404	arg1	indicators					406:415	liver tissue-related indicators	385:415	liver tissue-related indicators	385:415	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	6	11	theme	mice	1098:1101	arg1	serum					1060:1064	serum	1060:1064	serum	1060:1064	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	6	11	theme	mice	1098:1101	arg1	tissues					1076:1082	liver tissues	1070:1082	liver tissues	1070:1082	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	11	12	theme	Firmicutes	2001:2010	arg1	expression					2012:2021	Firmicutes expression	2001:2021	Firmicutes expression in the gut	2001:2032	A mouse faeces examination revealed that LDSB-KSFY07 could also upregulate Bacteroides, Lactobacterium, and Bifidobacterium microbial expression and downregulate Firmicutes expression in the gut.
35821704	8	13	theme	experimental	1218:1229	arg1	results					1231:1237	experimental results	1218:1237	experimental results	1218:1237	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	5	14	theme	activated	744:752	arg1	APTT					783:786	APTT	783:786	APTT	783:786	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	5	14	theme	activated	744:752	arg1	time					777:780	activated partial thromboplastin time	744:780	activated partial thromboplastin time (APTT)	744:787	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	8	15	theme	thrombotic	1387:1396	arg1	mice					1398:1401	thrombotic mice	1387:1401	thrombotic mice	1387:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	4	16	from	expression	486:495	arg1	experiments					620:630	quantitative polymerase chain reaction (qPCR) experiments	574:630	quantitative polymerase chain reaction (qPCR) experiments	574:630	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	4	16	from	expression	486:495	arg1	observations					556:567	slice observations	550:567	slice observations	550:567	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	4	16	from	expression	486:495	arg1	faeces					518:523	mouse faeces	512:523	mouse faeces using a biochemical kit	512:547	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	10	17	theme	plasminogen	1739:1749	arg1	t-PA					1762:1765	t-PA	1762:1765	t-PA	1762:1765	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	10	17	theme	plasminogen	1739:1749	arg1	activator					1751:1759	tissue plasminogen activator	1732:1759	tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice	1732:1836	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	5	18	theme	prothrombin	846:856	arg1	PT					864:865	PT	864:865	PT	864:865	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	5	18	theme	prothrombin	846:856	arg1	time					858:861	prothrombin time	846:861	prothrombin time (PT)	846:866	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	6	19	theme	malondialdehyde	924:938	arg1	levels					946:951	malondialdehyde (MDA) levels	924:951	malondialdehyde (MDA) levels	924:951	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	4	20	theme	microbes	500:507	arg1	expression					486:495	the expression	482:495	the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments	482:630	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	5	21	theme	thrombotic	717:726	arg1	mice					728:731	thrombotic mice	717:731	thrombotic mice	717:731	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	2	22	theme	inhibitory	237:246	arg1	effect					248:253	the inhibitory effect	233:253	the inhibitory effect of Lactobacillus delbrueckii subsp	233:288	A mouse thrombosis model was established by kappa-carrageenan to observe the inhibitory effect of Lactobacillus delbrueckii subsp.
35821704	1	23	theme	Damage	152:157	arg1	Regulation					128:137	the Regulation	124:137	the Regulation of Oxidative Damage	124:157	bulgaricus KSFY07 on Kappa-Carrageenan-Induced Thrombosis in Mice and the Regulation of Oxidative Damage.
35821704	1	23	theme	Damage	152:157	arg1	Thrombosis					101:110	Kappa-Carrageenan-Induced Thrombosis	75:110	Kappa-Carrageenan-Induced Thrombosis in Mice	75:118	bulgaricus KSFY07 on Kappa-Carrageenan-Induced Thrombosis in Mice and the Regulation of Oxidative Damage.
35821704	7	24	theme	tail	1187:1190	arg1	thrombosis					1197:1206	tail vein thrombosis	1187:1206	tail vein thrombosis	1187:1206	Pathological observations showed that LDSB-KSFY07 reduced liver tissue lesions and tail vein thrombosis.
35821704	2	25	theme	delbrueckii	272:282	arg1	subsp					284:288	Lactobacillus delbrueckii subsp	258:288	Lactobacillus delbrueckii subsp	258:288	A mouse thrombosis model was established by kappa-carrageenan to observe the inhibitory effect of Lactobacillus delbrueckii subsp.
35821704	5	26	theme	thrombotic	871:880	arg1	mice					882:885	thrombotic mice	871:885	thrombotic mice	871:885	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	3	27	theme	oxidative	345:353	arg1	response					362:369	the oxidative stress response	341:369	the oxidative stress response	341:369	bulgaricus KSFY07 (LDSB-KSFY07) on thrombosis and the oxidative stress response.
35821704	4	28	theme	biochemical	533:543	arg1	kit					545:547	a biochemical kit	531:547	a biochemical kit	531:547	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	8	29	theme	mRNA	1290:1293	arg1	expression					1295:1304	the mRNA expression	1286:1304	the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice	1286:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	11	30	from	expression	2012:2021	arg1	gut					2030:2032	the gut	2026:2032	the gut	2026:2032	A mouse faeces examination revealed that LDSB-KSFY07 could also upregulate Bacteroides, Lactobacterium, and Bifidobacterium microbial expression and downregulate Firmicutes expression in the gut.
35821704	5	31	theme	thrombin	804:811	arg1	TT					819:820	TT	819:820	TT	819:820	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	5	31	theme	thrombin	804:811	arg1	time					813:816	thrombin time	804:816	thrombin time (TT)	804:821	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	6	32	theme	dismutase	977:985	arg1	levels					1046:1051	superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels	966:1051	superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice	966:1101	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	7	33	theme	liver	1162:1166	arg1	lesions					1175:1181	liver tissue lesions	1162:1181	liver tissue lesions	1162:1181	Pathological observations showed that LDSB-KSFY07 reduced liver tissue lesions and tail vein thrombosis.
35821704	4	34	theme	mouse	512:516	arg1	faeces					518:523	mouse faeces	512:523	mouse faeces using a biochemical kit	512:547	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	12	35	theme	LDSB-KSFY07	2207:2217	arg1	concentrations					2189:2202	high concentrations	2184:2202	high concentrations of LDSB-KSFY07	2184:2217	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	9	36	theme	cell	1504:1507	arg1	molecule-1					1518:1527	intercellular cell adhesion molecule-1	1490:1527	intercellular cell adhesion molecule-1 (ICAM-1)	1490:1536	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	36	theme	cell	1504:1507	arg1	p65					1485:1487	NF-κB p65	1479:1487	NF-κB p65	1479:1487	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	36	theme	cell	1504:1507	arg1	ICAM-1					1530:1535	ICAM-1	1530:1535	ICAM-1	1530:1535	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	6	37	theme	peroxidase	1026:1035	arg1	levels					1046:1051	superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels	966:1051	superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice	966:1101	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	12	38	theme	better	2230:2235	arg1	response					2237:2244	a better response	2228:2244	a better response similar to that of the drug heparin	2228:2280	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	0	39	theme	Inhibitory	0:9	arg1	Effect					11:16	Inhibitory Effect	0:16	Inhibitory Effect of Lactobacillus delbrueckii	0:45	Inhibitory Effect of Lactobacillus delbrueckii subsp.
35821704	1	40	from	Regulation	128:137	arg1	Mice					115:118	Mice	115:118	Mice	115:118	bulgaricus KSFY07 on Kappa-Carrageenan-Induced Thrombosis in Mice and the Regulation of Oxidative Damage.
35821704	4	41	theme	polymerase	587:596	arg1	qPCR					614:617	qPCR	614:617	qPCR	614:617	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	4	41	theme	polymerase	587:596	arg1	reaction					604:611	quantitative polymerase chain reaction	574:611	quantitative polymerase chain reaction (qPCR) experiments	574:630	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	8	42	theme	manganese-SOD	1338:1350	arg1	expression					1295:1304	the mRNA expression	1286:1304	the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice	1286:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	7	43	theme	Pathological	1104:1115	arg1	observations					1117:1128	Pathological observations	1104:1128	Pathological observations	1104:1128	Pathological observations showed that LDSB-KSFY07 reduced liver tissue lesions and tail vein thrombosis.
35821704	10	44	from	expression	1768:1777	arg1	tissues					1811:1817	heart and tail vein vascular tissues	1782:1817	heart and tail vein vascular tissues	1782:1817	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	4	45	theme	reaction	604:611	arg1	experiments					620:630	quantitative polymerase chain reaction (qPCR) experiments	574:630	quantitative polymerase chain reaction (qPCR) experiments	574:630	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	11	46	theme	mouse	1841:1845	arg1	examination					1854:1864	A mouse faeces examination	1839:1864	A mouse faeces examination	1839:1864	A mouse faeces examination revealed that LDSB-KSFY07 could also upregulate Bacteroides, Lactobacterium, and Bifidobacterium microbial expression and downregulate Firmicutes expression in the gut.
35821704	0	47	theme	delbrueckii	35:45	arg1	Effect					11:16	Inhibitory Effect	0:16	Inhibitory Effect of Lactobacillus delbrueckii	0:45	Inhibitory Effect of Lactobacillus delbrueckii subsp.
35821704	12	48	theme	thrombus	2156:2163	arg1	mice					2165:2168	thrombus mice	2156:2168	thrombus mice	2156:2168	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	9	49	theme	cell	1548:1551	arg1	molecule-1					1562:1571	vascular cell adhesion molecule-1	1539:1571	vascular cell adhesion molecule-1 (VCAM-1)	1539:1580	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	49	theme	cell	1548:1551	arg1	p65					1485:1487	NF-κB p65	1479:1487	NF-κB p65	1479:1487	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	49	theme	cell	1548:1551	arg1	VCAM-1					1574:1579	VCAM-1	1574:1579	VCAM-1	1574:1579	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	4	50	theme	liver	385:389	arg1	indicators					406:415	liver tissue-related indicators	385:415	liver tissue-related indicators	385:415	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	8	51	theme	liver	1371:1375	arg1	tissue					1377:1382	the liver tissue	1367:1382	the liver tissue of thrombotic mice	1367:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	3	52	dep	KSFY07	302:307	arg1	bulgaricus					291:300	bulgaricus KSFY07 (LDSB-KSFY07) on thrombosis and the oxidative stress response	291:369	bulgaricus KSFY07 (LDSB-KSFY07) on thrombosis and the oxidative stress response	291:369	bulgaricus KSFY07 (LDSB-KSFY07) on thrombosis and the oxidative stress response.
35821704	10	53	theme	vein	1797:1800	arg1	tissues					1811:1817	heart and tail vein vascular tissues	1782:1817	heart and tail vein vascular tissues	1782:1817	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	12	54	theme	oxidative	2129:2137	arg1	damage					2146:2151	liver oxidative stress damage	2123:2151	liver oxidative stress damage in thrombus mice	2123:2168	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	10	55	theme	plasminogen	1663:1673	arg1	PAI-1					1698:1702	PAI-1	1698:1702	PAI-1	1698:1702	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	10	55	theme	plasminogen	1663:1673	arg1	inhibitor-1					1685:1695	plasminogen activator inhibitor-1	1663:1695	plasminogen activator inhibitor-1 (PAI-1) mRNA expression	1663:1719	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	8	56	from	expression	1295:1304	arg1	tissue					1377:1382	the liver tissue	1367:1382	the liver tissue of thrombotic mice	1367:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	6	57	from	levels	1046:1051	arg1	serum					1060:1064	serum	1060:1064	serum	1060:1064	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	6	57	from	levels	1046:1051	arg1	tissues					1076:1082	liver tissues	1070:1082	liver tissues	1070:1082	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	1	58	from	Thrombosis	101:110	arg1	Mice					115:118	Mice	115:118	Mice	115:118	bulgaricus KSFY07 on Kappa-Carrageenan-Induced Thrombosis in Mice and the Regulation of Oxidative Damage.
35821704	12	59	theme	similar	2246:2252	arg1	response					2237:2244	a better response	2228:2244	a better response similar to that of the drug heparin	2228:2280	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	4	60	theme	microbial	433:441	arg1	composition					443:453	intestinal microbial composition	422:453	intestinal microbial composition	422:453	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	10	61	theme	thrombotic	1822:1831	arg1	mice					1833:1836	thrombotic mice	1822:1836	thrombotic mice	1822:1836	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	5	62	theme	black	703:707	arg1	tail					709:712	black tail	703:712	black tail	703:712	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	11	63	theme	Bifidobacterium	1947:1961	arg1	expression					1973:1982	Bacteroides, Lactobacterium, and Bifidobacterium microbial expression	1914:1982	expression	1973:1982	A mouse faeces examination revealed that LDSB-KSFY07 could also upregulate Bacteroides, Lactobacterium, and Bifidobacterium microbial expression and downregulate Firmicutes expression in the gut.
35821704	2	64	theme	mouse	162:166	arg1	model					179:183	A mouse thrombosis model	160:183	A mouse thrombosis model	160:183	A mouse thrombosis model was established by kappa-carrageenan to observe the inhibitory effect of Lactobacillus delbrueckii subsp.
35821704	10	65	theme	inhibitor-1	1685:1695	arg1	expression					1710:1719	plasminogen activator inhibitor-1 (PAI-1) mRNA expression	1663:1719	plasminogen activator inhibitor-1 (PAI-1) mRNA expression	1663:1719	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	9	66	theme	tail	1601:1604	arg1	tissue					1620:1625	tail vein vascular tissue	1601:1625	tail vein vascular tissue	1601:1625	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	67	theme	vascular	1611:1618	arg1	tissue					1620:1625	tail vein vascular tissue	1601:1625	tail vein vascular tissue	1601:1625	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	6	68	theme	thrombotic	1087:1096	arg1	mice					1098:1101	thrombotic mice	1087:1101	thrombotic mice	1087:1101	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	12	69	theme	mouse	2095:2099	arg1	thrombosis					2101:2110	mouse thrombosis	2095:2110	mouse thrombosis	2095:2110	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	10	70	theme	mRNA	1705:1708	arg1	expression					1710:1719	plasminogen activator inhibitor-1 (PAI-1) mRNA expression	1663:1719	plasminogen activator inhibitor-1 (PAI-1) mRNA expression	1663:1719	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	8	71	theme	mice	1398:1401	arg1	tissue					1377:1382	the liver tissue	1367:1382	the liver tissue of thrombotic mice	1367:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	5	72	theme	partial	754:760	arg1	APTT					783:786	APTT	783:786	APTT	783:786	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	5	72	theme	partial	754:760	arg1	time					777:780	activated partial thromboplastin time	744:780	activated partial thromboplastin time (APTT)	744:787	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	10	73	theme	activator	1751:1759	arg1	expression					1768:1777	tissue plasminogen activator (t-PA) expression	1732:1777	tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice	1732:1836	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	10	74	theme	tissue	1732:1737	arg1	t-PA					1762:1765	t-PA	1762:1765	t-PA	1762:1765	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	10	74	theme	tissue	1732:1737	arg1	activator					1751:1759	tissue plasminogen activator	1732:1759	tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice	1732:1836	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	12	75	theme	drug	2269:2272	arg1	heparin					2274:2280	the drug heparin	2265:2280	the drug heparin	2265:2280	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	6	76	dep	serum	1060:1064	arg1	the					1056:1058	the	1056:1058	the	1056:1058	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	1	77	theme	Oxidative	142:150	arg1	Damage					152:157	Oxidative Damage	142:157	Oxidative Damage	142:157	bulgaricus KSFY07 on Kappa-Carrageenan-Induced Thrombosis in Mice and the Regulation of Oxidative Damage.
35821704	6	78	theme	catalase	994:1001	arg1	levels					1046:1051	superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels	966:1051	superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice	966:1101	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	2	79	theme	subsp	284:288	arg1	effect					248:253	the inhibitory effect	233:253	the inhibitory effect of Lactobacillus delbrueckii subsp	233:288	A mouse thrombosis model was established by kappa-carrageenan to observe the inhibitory effect of Lactobacillus delbrueckii subsp.
35821704	9	80	theme	mRNA	1460:1463	arg1	expression					1465:1474	the mRNA expression	1456:1474	the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue	1456:1625	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	7	81	theme	tissue	1168:1173	arg1	lesions					1175:1181	liver tissue lesions	1162:1181	liver tissue lesions	1162:1181	Pathological observations showed that LDSB-KSFY07 reduced liver tissue lesions and tail vein thrombosis.
35821704	3	82	theme	stress	355:360	arg1	response					362:369	the oxidative stress response	341:369	the oxidative stress response	341:369	bulgaricus KSFY07 (LDSB-KSFY07) on thrombosis and the oxidative stress response.
35821704	4	83	theme	slice	550:554	arg1	observations					556:567	slice observations	550:567	slice observations	550:567	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	9	84	theme	p65	1485:1487	arg1	expression					1465:1474	the mRNA expression	1456:1474	the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue	1456:1625	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	6	85	theme	superoxide	966:975	arg1	dismutase					977:985	superoxide dismutase	966:985	superoxide dismutase (SOD)	966:991	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	6	85	theme	superoxide	966:975	arg1	SOD					988:990	SOD	988:990	SOD	988:990	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	12	86	theme	high	2184:2187	arg1	concentrations					2189:2202	high concentrations	2184:2202	high concentrations of LDSB-KSFY07	2184:2217	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	9	87	theme	intercellular	1490:1502	arg1	molecule-1					1518:1527	intercellular cell adhesion molecule-1	1490:1527	intercellular cell adhesion molecule-1 (ICAM-1)	1490:1536	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	87	theme	intercellular	1490:1502	arg1	p65					1485:1487	NF-κB p65	1479:1487	NF-κB p65	1479:1487	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	87	theme	intercellular	1490:1502	arg1	ICAM-1					1530:1535	ICAM-1	1530:1535	ICAM-1	1530:1535	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	8	88	theme	copper/zinc-SOD	1309:1323	arg1	expression					1295:1304	the mRNA expression	1286:1304	the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice	1286:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	9	89	theme	adhesion	1509:1516	arg1	molecule-1					1518:1527	intercellular cell adhesion molecule-1	1490:1527	intercellular cell adhesion molecule-1 (ICAM-1)	1490:1536	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	89	theme	adhesion	1509:1516	arg1	p65					1485:1487	NF-κB p65	1479:1487	NF-κB p65	1479:1487	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	89	theme	adhesion	1509:1516	arg1	ICAM-1					1530:1535	ICAM-1	1530:1535	ICAM-1	1530:1535	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	6	90	theme	glutathione	1014:1024	arg1	GSH-Px					1038:1043	GSH-Px	1038:1043	GSH-Px	1038:1043	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	6	90	theme	glutathione	1014:1024	arg1	peroxidase					1026:1035	glutathione peroxidase	1014:1035	glutathione peroxidase (GSH-Px)	1014:1044	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	4	91	theme	chain	598:602	arg1	qPCR					614:617	qPCR	614:617	qPCR	614:617	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	4	91	theme	chain	598:602	arg1	reaction					604:611	quantitative polymerase chain reaction	574:611	quantitative polymerase chain reaction (qPCR) experiments	574:630	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	2	92	theme	Lactobacillus	258:270	arg1	subsp					284:288	Lactobacillus delbrueckii subsp	258:288	Lactobacillus delbrueckii subsp	258:288	A mouse thrombosis model was established by kappa-carrageenan to observe the inhibitory effect of Lactobacillus delbrueckii subsp.
35821704	1	93	theme	Kappa-Carrageenan-Induced	75:99	arg1	Thrombosis					101:110	Kappa-Carrageenan-Induced Thrombosis	75:110	Kappa-Carrageenan-Induced Thrombosis in Mice	75:118	bulgaricus KSFY07 on Kappa-Carrageenan-Induced Thrombosis in Mice and the Regulation of Oxidative Damage.
35821704	10	94	theme	tail	1792:1795	arg1	vein					1797:1800	tail vein	1792:1800	tail vein	1792:1800	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	10	95	theme	heart	1782:1786	arg1	tissues					1811:1817	heart and tail vein vascular tissues	1782:1817	heart and tail vein vascular tissues	1782:1817	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	12	96	theme	stress	2139:2144	arg1	damage					2146:2151	liver oxidative stress damage	2123:2151	liver oxidative stress damage in thrombus mice	2123:2168	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	11	97	theme	faeces	1847:1852	arg1	examination					1854:1864	A mouse faeces examination	1839:1864	A mouse faeces examination	1839:1864	A mouse faeces examination revealed that LDSB-KSFY07 could also upregulate Bacteroides, Lactobacterium, and Bifidobacterium microbial expression and downregulate Firmicutes expression in the gut.
35821704	9	98	theme	vascular	1539:1546	arg1	molecule-1					1562:1571	vascular cell adhesion molecule-1	1539:1571	vascular cell adhesion molecule-1 (VCAM-1)	1539:1580	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	98	theme	vascular	1539:1546	arg1	p65					1485:1487	NF-κB p65	1479:1487	NF-κB p65	1479:1487	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	98	theme	vascular	1539:1546	arg1	VCAM-1					1574:1579	VCAM-1	1574:1579	VCAM-1	1574:1579	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	4	99	theme	Mouse	372:376	arg1	serum					378:382	Mouse serum	372:382	Mouse serum	372:382	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	6	100	theme	liver	1070:1074	arg1	tissues					1076:1082	liver tissues	1070:1082	liver tissues	1070:1082	LDSB-KSFY07 was also able to reduce malondialdehyde (MDA) levels and increase superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver tissues of thrombotic mice.
35821704	5	101	theme	thromboplastin	762:775	arg1	APTT					783:786	APTT	783:786	APTT	783:786	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	5	101	theme	thromboplastin	762:775	arg1	time					777:780	activated partial thromboplastin time	744:780	activated partial thromboplastin time (APTT)	744:787	The results showed that LDSB-KSFY07 effectively reduced the degree of black tail in thrombotic mice, increased activated partial thromboplastin time (APTT), and decreased thrombin time (TT), fibrinogen (FIB), and prothrombin time (PT) in thrombotic mice.
35821704	4	102	theme	quantitative	574:585	arg1	qPCR					614:617	qPCR	614:617	qPCR	614:617	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	4	102	theme	quantitative	574:585	arg1	reaction					604:611	quantitative polymerase chain reaction	574:611	quantitative polymerase chain reaction (qPCR) experiments	574:630	Mouse serum, liver tissue-related indicators, and intestinal microbial composition were measured by examining the expression of microbes in mouse faeces using a biochemical kit, slice observations, and quantitative polymerase chain reaction (qPCR) experiments.
35821704	8	103	theme	GSH-Px	1357:1362	arg1	expression					1295:1304	the mRNA expression	1286:1304	the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice	1286:1401	Further, experimental results showed that LDSB-KSFY07 was able to upregulate the mRNA expression of copper/zinc-SOD (Cu/Zn-SOD), manganese-SOD, and GSH-Px in the liver tissue of thrombotic mice.
35821704	9	104	theme	adhesion	1553:1560	arg1	molecule-1					1562:1571	vascular cell adhesion molecule-1	1539:1571	vascular cell adhesion molecule-1 (VCAM-1)	1539:1580	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	104	theme	adhesion	1553:1560	arg1	p65					1485:1487	NF-κB p65	1479:1487	NF-κB p65	1479:1487	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	9	104	theme	adhesion	1553:1560	arg1	VCAM-1					1574:1579	VCAM-1	1574:1579	VCAM-1	1574:1579	Moreover, LDSB-KSFY07 was also able to downregulate the mRNA expression of NF-κB p65, intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in tail vein vascular tissue.
35821704	10	105	theme	vascular	1802:1809	arg1	tissues					1811:1817	heart and tail vein vascular tissues	1782:1817	heart and tail vein vascular tissues	1782:1817	Meanwhile, LDSB-KSFY07 could raise plasminogen activator inhibitor-1 (PAI-1) mRNA expression and reduce tissue plasminogen activator (t-PA) expression in heart and tail vein vascular tissues of thrombotic mice.
35821704	12	106	from	damage	2146:2151	arg1	mice					2165:2168	thrombus mice	2156:2168	thrombus mice	2156:2168	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
35821704	12	107	theme	liver	2123:2127	arg1	damage					2146:2151	liver oxidative stress damage	2123:2151	liver oxidative stress damage in thrombus mice	2123:2168	These results indicate that LDSB-KSFY07 was able to inhibit mouse thrombosis and reduce liver oxidative stress damage in thrombus mice and show that high concentrations of LDSB-KSFY07 provided a better response similar to that of the drug heparin.
37239819	6	0	gly	glycosylation	1603:1615	arg1	glycoprotein					1633:1644	alpha-1 acid glycoprotein	1620:1644	alpha-1 acid glycoprotein	1620:1644	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	6	1	from	significance	1561:1572	arg1	general					1649:1655	general	1649:1655	general	1649:1655	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	6	1	from	significance	1561:1572	arg1	glycosylation					1603:1615	glycosylation	1603:1615	glycosylation	1603:1615	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	6	1	from	significance	1561:1572	arg1	sialylation					1588:1598	sialylation	1588:1598	sialylation	1588:1598	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	5	2	gly	glycoprotein	1387:1398	arg1	glycoprotein					1387:1398	native alpha-1 acid glycoprotein	1367:1398	native alpha-1 acid glycoprotein	1367:1398	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	1	3	theme	drugs	285:289	arg1	binding					255:261	binding	255:261	binding	255:261	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	3	theme	drugs	285:289	arg1	transport					267:275	transport	267:275	transport	267:275	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	6	4	theme	glycoprotein	1633:1644	arg1	glycosylation					1603:1615	glycosylation	1603:1615	glycosylation	1603:1615	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	6	4	theme	glycoprotein	1633:1644	arg1	sialylation					1588:1598	sialylation	1588:1598	sialylation	1588:1598	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	4	5	theme	heat	898:901	arg1	heat					898:901	heat	898:901	heat released or absorbed during the association processes of biomolecules in solution	898:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	4	5	theme	heat	898:901	arg1	amount					888:893	the amount	884:893	the amount of heat released or absorbed during the association processes of biomolecules in solution	884:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	6	6	theme	alpha-1	1620:1626	arg1	glycoprotein					1633:1644	alpha-1 acid glycoprotein	1620:1644	alpha-1 acid glycoprotein	1620:1644	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	2	7	theme	acid	552:555	arg1	glycoprotein					557:568	alpha-1 acid glycoprotein	544:568	alpha-1 acid glycoprotein	544:568	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	3	8	gly	desialylated	605:616	arg1	glycoprotein					631:642	native or desialylated alpha-1 acid glycoprotein	595:642	native or desialylated alpha-1 acid glycoprotein	595:642	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	1	9	theme	plasma	224:229	arg1	protein					231:237	an acute-phase plasma protein	209:237	an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances	209:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	9	theme	plasma	224:229	arg1	glycoprotein					193:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	4	10	from	biomolecules	960:971	arg1	solution					976:983	solution	976:983	solution	976:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	5	11	theme	enthalpy-driven	1136:1150	arg1	interactions					1163:1174	enthalpy-driven exothermic interactions	1136:1174	enthalpy-driven exothermic interactions	1136:1174	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	5	11	theme	enthalpy-driven	1136:1150	arg1	binding					1083:1089	the binding	1079:1089	the binding of drugs with alpha-1 acid glycoprotein	1079:1129	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	3	12	theme	desialylated	605:616	arg1	glycoprotein					631:642	native or desialylated alpha-1 acid glycoprotein	595:642	native or desialylated alpha-1 acid glycoprotein	595:642	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	4	13	dep	approach	855:862	arg1	estimate					1007:1014	estimate	1007:1014	to quantitatively estimate the thermodynamics of the interaction	989:1052	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	4	13	dep	approach	855:862	arg1	measure					876:882	measure	876:882	to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution	864:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	4	14	theme	association	935:945	arg1	processes					947:955	the association processes	931:955	the association processes of biomolecules in solution	931:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	5	15	theme	drugs	1094:1098	arg1	interactions					1163:1174	enthalpy-driven exothermic interactions	1136:1174	enthalpy-driven exothermic interactions	1136:1174	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	5	15	theme	drugs	1094:1098	arg1	binding					1083:1089	the binding	1079:1089	the binding of drugs with alpha-1 acid glycoprotein	1079:1129	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	3	16	theme	native	595:600	arg1	glycoprotein					631:642	native or desialylated alpha-1 acid glycoprotein	595:642	native or desialylated alpha-1 acid glycoprotein	595:642	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	5	17	theme	alpha-1	1105:1111	arg1	glycoprotein					1118:1129	alpha-1 acid glycoprotein	1105:1129	alpha-1 acid glycoprotein	1105:1129	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	5	18	theme	alpha-1	1374:1380	arg1	glycoprotein					1387:1398	native alpha-1 acid glycoprotein	1367:1398	native alpha-1 acid glycoprotein	1367:1398	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	0	19	theme	Serum	62:66	arg1	Interactions					77:88	Human Serum AAG-Drug Interactions	56:88	Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry	56:133	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	5	20	theme	binding	1185:1191	arg1	affinity					1193:1200	the binding affinity	1181:1200	the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1181:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	3	21	theme	acid	626:629	arg1	glycoprotein					631:642	native or desialylated alpha-1 acid glycoprotein	595:642	native or desialylated alpha-1 acid glycoprotein	595:642	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	1	22	theme	Human	168:172	arg1	protein					231:237	an acute-phase plasma protein	209:237	an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances	209:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	22	theme	Human	168:172	arg1	glycoprotein					193:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	4	23	theme	interaction	1042:1052	arg1	thermodynamics					1020:1033	the thermodynamics	1016:1033	the thermodynamics of the interaction	1016:1052	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	1	24	theme	basic	303:307	arg1	substances					324:333	basic and lipophilic substances	303:333	basic and lipophilic substances	303:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	24	theme	basic	303:307	arg1	drugs					285:289	many drugs	280:289	many drugs	280:289	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	3	25	theme	titration	768:776	arg1	calorimetry					778:788	isothermal titration calorimetry	757:788	isothermal titration calorimetry	757:788	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	1	26	theme	alpha-1	180:186	arg1	protein					231:237	an acute-phase plasma protein	209:237	an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances	209:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	26	theme	alpha-1	180:186	arg1	glycoprotein					193:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	2	27	theme	drug	528:531	arg1	binding					533:539	drug binding	528:539	drug binding to alpha-1 acid glycoprotein	528:568	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	2	28	theme	N-glycan	404:411	arg1	chains					413:418	the N-glycan chains	400:418	the N-glycan chains of alpha-1 acid glycoprotein change	400:454	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	0	29	theme	Titration	113:121	arg1	Calorimetry					123:133	Isothermal Titration Calorimetry	102:133	Isothermal Titration Calorimetry	102:133	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	5	30	gly	glycoprotein	1261:1272	arg1	glycoprotein					1261:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	5	31	theme	significant	1431:1441	arg1	difference					1443:1452	no significant difference	1428:1452	no significant difference	1428:1452	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	0	32	theme	Potential	0:8	arg1	Effects					30:36	Potential Clinically Relevant Effects	0:36	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?	0:166	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	5	33	theme	M.	1232:1233	arg1	glycoprotein					1261:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	6	34	gly	glycoprotein	1633:1644	arg1	glycoprotein					1633:1644	alpha-1 acid glycoprotein	1620:1644	alpha-1 acid glycoprotein	1620:1644	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	2	35	theme	change	449:454	arg1	chains					413:418	the N-glycan chains	400:418	the N-glycan chains of alpha-1 acid glycoprotein change	400:454	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	2	36	theme	acid	431:434	arg1	change					449:454	alpha-1 acid glycoprotein change	423:454	alpha-1 acid glycoprotein change	423:454	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	0	37	theme	Relevant	21:28	arg1	Effects					30:36	Potential Clinically Relevant Effects	0:36	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?	0:166	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	5	38	theme	alpha-1	1248:1254	arg1	glycoprotein					1261:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	1	39	theme	lipophilic	313:322	arg1	substances					324:333	basic and lipophilic substances	303:333	basic and lipophilic substances	303:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	39	theme	lipophilic	313:322	arg1	drugs					285:289	many drugs	280:289	many drugs	280:289	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	2	40	theme	certain	471:477	arg1	conditions					486:495	certain health conditions	471:495	certain health conditions	471:495	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	3	41	theme	representative	653:666	arg1	drugs-clindamycin					668:684	drugs-clindamycin	668:684	drugs-clindamycin	668:684	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	6	42	theme	sialylation	1488:1498	arg1	degree					1478:1483	a different degree	1466:1483	a different degree of sialylation	1466:1498	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	4	43	theme	used	850:853	arg1	assay					807:811	The calorimetry assay	791:811	The calorimetry assay used here	791:821	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	4	43	theme	used	850:853	arg1	approach					855:862	a convenient and widely used approach	826:862	a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction	826:1052	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	5	44	theme	different	1295:1303	arg1	binding					1305:1311	significantly different binding	1281:1311	significantly different binding	1281:1311	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	1	45	gly	glycoprotein	193:204	arg1	protein					231:237	an acute-phase plasma protein	209:237	an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances	209:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	45	gly	glycoprotein	193:204	arg1	glycoprotein					193:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	2	46	theme	sialic	366:371	arg1	groups					378:383	the sialic acid groups	362:383	the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein	362:568	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	6	47	theme	clinical	1552:1559	arg1	significance					1561:1572	the clinical significance	1548:1572	the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general	1548:1655	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	5	48	theme	glycoprotein	1261:1272	arg1	range					1213:1217	the range	1209:1217	the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1209:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	5	49	with	binding	1083:1089	arg1	glycoprotein					1118:1129	alpha-1 acid glycoprotein	1105:1129	alpha-1 acid glycoprotein	1105:1129	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	1	50	theme	many	280:283	arg1	substances					324:333	basic and lipophilic substances	303:333	basic and lipophilic substances	303:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	50	theme	many	280:283	arg1	drugs					285:289	many drugs	280:289	many drugs	280:289	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	51	theme	acid	188:191	arg1	protein					231:237	an acute-phase plasma protein	209:237	an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances	209:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	51	theme	acid	188:191	arg1	glycoprotein					193:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	3	52	gly	glycoprotein	631:642	arg1	glycoprotein					631:642	native or desialylated alpha-1 acid glycoprotein	595:642	native or desialylated alpha-1 acid glycoprotein	595:642	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	3	53	theme	isothermal	757:766	arg1	calorimetry					778:788	isothermal titration calorimetry	757:788	isothermal titration calorimetry	757:788	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	6	54	theme	acid	1628:1631	arg1	glycoprotein					1633:1644	alpha-1 acid glycoprotein	1620:1644	alpha-1 acid glycoprotein	1620:1644	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	1	55	theme	acute-phase	212:222	arg1	protein					231:237	an acute-phase plasma protein	209:237	an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances	209:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	55	theme	acute-phase	212:222	arg1	glycoprotein					193:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	2	56	theme	alpha-1	544:550	arg1	glycoprotein					557:568	alpha-1 acid glycoprotein	544:568	alpha-1 acid glycoprotein	544:568	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	1	57	dep	binding	255:261	arg1	the					251:253	the	251:253	the	251:253	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	5	58	dep	showed	1067:1072	arg1	whereas					1401:1407	whereas	1401:1407	whereas	1401:1407	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	4	59	from	solution	976:983	arg1	processes					947:955	the association processes	931:955	the association processes of biomolecules in solution	931:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	5	60	gly	Desialylated	1235:1246	arg1	glycoprotein					1261:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	5	61	theme	exothermic	1152:1161	arg1	interactions					1163:1174	enthalpy-driven exothermic interactions	1136:1174	enthalpy-driven exothermic interactions	1136:1174	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	5	61	theme	exothermic	1152:1161	arg1	binding					1083:1089	the binding	1079:1089	the binding of drugs with alpha-1 acid glycoprotein	1079:1129	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	4	62	from	processes	947:955	arg1	solution					976:983	solution	976:983	solution	976:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	2	63	gly	glycoprotein	557:568	arg1	glycoprotein					557:568	alpha-1 acid glycoprotein	544:568	alpha-1 acid glycoprotein	544:568	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	5	64	theme	native	1367:1372	arg1	glycoprotein					1387:1398	native alpha-1 acid glycoprotein	1367:1398	native alpha-1 acid glycoprotein	1367:1398	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	0	65	theme	AAG-Drug	68:75	arg1	Interactions					77:88	Human Serum AAG-Drug Interactions	56:88	Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry	56:133	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	5	66	theme	acid	1113:1116	arg1	glycoprotein					1118:1129	alpha-1 acid glycoprotein	1105:1129	alpha-1 acid glycoprotein	1105:1129	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	3	67	theme	alpha-1	618:624	arg1	glycoprotein					631:642	native or desialylated alpha-1 acid glycoprotein	595:642	native or desialylated alpha-1 acid glycoprotein	595:642	The interaction between native or desialylated alpha-1 acid glycoprotein and four representative drugs-clindamycin, diltiazem, lidocaine, and warfarin-was quantitatively evaluated using isothermal titration calorimetry.
37239819	0	68	from	Effects	30:36	arg1	Interactions					77:88	Human Serum AAG-Drug Interactions	56:88	Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry	56:133	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	6	69	theme	different	1468:1476	arg1	degree					1478:1483	a different degree	1466:1483	a different degree of sialylation	1466:1498	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	1	70	theme	serum	174:178	arg1	protein					231:237	an acute-phase plasma protein	209:237	an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances	209:333	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	1	70	theme	serum	174:178	arg1	glycoprotein					193:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein	168:204	Human serum alpha-1 acid glycoprotein is an acute-phase plasma protein involved in the binding and transport of many drugs, especially basic and lipophilic substances.
37239819	4	71	theme	biomolecules	960:971	arg1	processes					947:955	the association processes	931:955	the association processes of biomolecules in solution	931:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	6	72	theme	different	1514:1522	arg1	affinities					1532:1541	different binding affinities	1514:1541	different binding affinities	1514:1541	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	0	73	theme	Isothermal	102:111	arg1	Calorimetry					123:133	Isothermal Titration Calorimetry	102:133	Isothermal Titration Calorimetry	102:133	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	2	74	dep	conditions	486:495	arg1	response					459:466	response	459:466	response	459:466	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	6	75	gly	sialylation	1588:1598	arg1	glycoprotein					1633:1644	alpha-1 acid glycoprotein	1620:1644	alpha-1 acid glycoprotein	1620:1644	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	2	76	theme	major	512:516	arg1	impact					518:523	a major impact	510:523	a major impact	510:523	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	4	77	dep	heat	898:901	arg1	absorbed					915:922	absorbed	915:922	absorbed during the association processes of biomolecules in solution	915:983	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	4	77	dep	heat	898:901	arg1	released					903:910	released	903:910	released	903:910	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	6	78	from	glycosylation	1603:1615	arg1	significance					1561:1572	the clinical significance	1548:1572	the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general	1548:1655	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	5	79	theme	acid	1382:1385	arg1	glycoprotein					1387:1398	native alpha-1 acid glycoprotein	1367:1398	native alpha-1 acid glycoprotein	1367:1398	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	6	80	theme	binding	1524:1530	arg1	affinities					1532:1541	different binding affinities	1514:1541	different binding affinities	1514:1541	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	5	81	theme	Desialylated	1235:1246	arg1	glycoprotein					1261:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	2	82	theme	glycoprotein	436:447	arg1	change					449:454	alpha-1 acid glycoprotein change	423:454	alpha-1 acid glycoprotein change	423:454	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	4	83	theme	calorimetry	795:805	arg1	assay					807:811	The calorimetry assay	791:811	The calorimetry assay used here	791:821	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	4	83	theme	calorimetry	795:805	arg1	approach					855:862	a convenient and widely used approach	826:862	a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction	826:1052	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	2	84	theme	alpha-1	423:429	arg1	change					449:454	alpha-1 acid glycoprotein change	423:454	alpha-1 acid glycoprotein change	423:454	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	0	85	theme	Sialylation	41:51	arg1	Effects					30:36	Potential Clinically Relevant Effects	0:36	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?	0:166	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	5	86	theme	acid	1256:1259	arg1	glycoprotein					1261:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	6	87	from	changes	1577:1583	arg1	glycosylation					1603:1615	glycosylation	1603:1615	glycosylation	1603:1615	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	6	87	from	changes	1577:1583	arg1	sialylation					1588:1598	sialylation	1588:1598	sialylation	1588:1598	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	2	88	theme	health	479:484	arg1	conditions					486:495	certain health conditions	471:495	certain health conditions	471:495	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	0	89	theme	Human	56:60	arg1	Interactions					77:88	Human Serum AAG-Drug Interactions	56:88	Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry	56:133	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	6	90	from	sialylation	1588:1598	arg1	significance					1561:1572	the clinical significance	1548:1572	the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general	1548:1655	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	2	91	contain	have	505:508	arg2	impact					518:523	a major impact	510:523	a major impact	510:523	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	2	91	contain	have	505:508	arg1	groups					378:383	the sialic acid groups	362:383	the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein	362:568	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	5	92	theme	10-5-10-6	1222:1230	arg1	glycoprotein					1261:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	10-5-10-6 M. Desialylated alpha-1 acid glycoprotein	1222:1272	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	0	93	dep	Effects	30:36	arg1	Insight					136:142	Insight	136:142	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?	0:166	Potential Clinically Relevant Effects of Sialylation on Human Serum AAG-Drug Interactions Assessed by Isothermal Titration Calorimetry: Insight into Pharmacoglycomics?
37239819	5	94	gly	glycoprotein	1118:1129	arg1	glycoprotein					1118:1129	alpha-1 acid glycoprotein	1105:1129	alpha-1 acid glycoprotein	1105:1129	The results showed that the binding of drugs with alpha-1 acid glycoprotein were enthalpy-driven exothermic interactions, and the binding affinity was in the range of 10-5-10-6 M. Desialylated alpha-1 acid glycoprotein showed significantly different binding with diltiazem, lidocaine, and warfarin compared with native alpha-1 acid glycoprotein, whereas clindamycin showed no significant difference.
37239819	4	95	theme	convenient	828:837	arg1	assay					807:811	The calorimetry assay	791:811	The calorimetry assay used here	791:821	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	4	95	theme	convenient	828:837	arg1	approach					855:862	a convenient and widely used approach	826:862	a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction	826:1052	The calorimetry assay used here is a convenient and widely used approach to directly measure the amount of heat released or absorbed during the association processes of biomolecules in solution and to quantitatively estimate the thermodynamics of the interaction.
37239819	6	96	theme	changes	1577:1583	arg1	significance					1561:1572	the clinical significance	1548:1572	the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general	1548:1655	Therefore, a different degree of sialylation may result in different binding affinities, and the clinical significance of changes in sialylation or glycosylation of alpha-1 acid glycoprotein in general should not be neglected.
37239819	2	97	gly	glycoprotein	436:447	arg1	glycoprotein					436:447	alpha-1 acid glycoprotein change	423:454	alpha-1 acid glycoprotein change	423:454	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
37239819	2	98	theme	acid	373:376	arg1	groups					378:383	the sialic acid groups	362:383	the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein	362:568	It has been reported that the sialic acid groups that terminate the N-glycan chains of alpha-1 acid glycoprotein change in response to certain health conditions and may have a major impact on drug binding to alpha-1 acid glycoprotein.
36874224	5	0	theme	carbohydrates	772:784	arg1	content					761:767	the content	757:767	the content of carbohydrates (total sugar)	757:798	Chemical analysis indicated that the content of carbohydrates (total sugar) increased with processing temperature, while that of uronic acid decreased.
36874224	9	1	attach	isolated	1545:1552	arg2	polysaccharides					1529:1543	the polysaccharides	1525:1543	the polysaccharides isolated from ginseng	1525:1565	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	9	1	attach	isolated	1545:1552	arg1	ginseng					1559:1565	ginseng	1559:1565	ginseng	1559:1565	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	6	2	theme	necrosis	971:978	arg1	alpha					987:991	tumor necrosis factor alpha	965:991	tumor necrosis factor alpha (TNF-α)	965:999	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	6	2	theme	necrosis	971:978	arg1	TNF-α					994:998	TNF-α	994:998	TNF-α	994:998	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	8	3	theme	intracellular	1260:1272	arg1	pathways					1284:1291	intracellular signaling pathways	1260:1291	intracellular signaling pathways	1260:1291	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	9	4	theme	heat	1608:1611	arg1	processing					1613:1622	heat processing	1608:1622	heat processing	1608:1622	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	1	5	theme	significant	176:186	arg1	issue					202:206	a significant public health issue	174:206	a significant public health issue	174:206	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	1	5	theme	significant	176:186	arg1	persistence					136:146	The worldwide persistence	122:146	The worldwide persistence of infectious diseases	122:169	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	6	6	theme	alpha	987:991	arg1	levels					1024:1029	tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels	965:1029	tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages	965:1054	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	7	7	theme	synthase	1160:1167	arg1	highest					1202:1208	highest	1202:1208	highest	1202:1208	The expression of inducible NO synthase, which affects NO secretion, was highest in the macrophages treated with P-WG.
36874224	7	7	theme	synthase	1160:1167	arg1	expression					1133:1142	The expression	1129:1142	The expression of inducible NO synthase, which affects NO secretion,	1129:1196	The expression of inducible NO synthase, which affects NO secretion, was highest in the macrophages treated with P-WG.
36874224	6	8	from	levels	1024:1029	arg1	macrophages					1044:1054	RAW 264.7 macrophages	1034:1054	RAW 264.7 macrophages	1034:1054	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	3	9	theme	immunostimulatory	527:543	arg1	activity					545:552	immunostimulatory activity	527:552	immunostimulatory activity against RAW 264.7 murine macrophages	527:589	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	6	10	theme	factor	980:985	arg1	alpha					987:991	tumor necrosis factor alpha	965:991	tumor necrosis factor alpha (TNF-α)	965:999	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	6	10	theme	factor	980:985	arg1	TNF-α					994:998	TNF-α	994:998	TNF-α	994:998	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	0	11	theme	heat	105:108	arg1	processing					110:119	heat processing	105:119	heat processing	105:119	Characterization of macrophage activation after treatment with polysaccharides from ginseng according to heat processing.
36874224	3	12	from	types	393:397	arg1	P-RG					442:445	P-RG	442:445	P-RG	442:445	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	12	from	types	393:397	arg1	ginseng					476:482	heat-processed (P-HPG) ginseng	453:482	heat-processed (P-HPG) ginseng	453:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	12	from	types	393:397	arg1	P-WG					430:433	P-WG	430:433	P-WG	430:433	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	12	from	types	393:397	arg1	red					437:439	red	437:439	red	437:439	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	12	from	types	393:397	arg1	white					423:427	white	423:427	white	423:427	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	2	13	from	present	261:267	arg1	ginseng					298:304	ginseng	298:304	ginseng	298:304	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	2	13	from	present	261:267	arg1	products					280:287	natural products	272:287	natural products	272:287	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	3	14	theme	P-HPG	469:473	arg1	ginseng					476:482	heat-processed (P-HPG) ginseng	453:482	heat-processed (P-HPG) ginseng	453:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	7	15	theme	inducible	1147:1155	arg1	synthase					1160:1167	inducible NO synthase	1147:1167	inducible NO synthase	1147:1167	The expression of inducible NO synthase, which affects NO secretion, was highest in the macrophages treated with P-WG.
36874224	6	16	theme	interleukin	1005:1015	arg1	IL					1018:1019	interleukin (IL)-6	1005:1022	interleukin (IL)-6	1005:1022	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	1	17	theme	public	188:193	arg1	issue					202:206	a significant public health issue	174:206	a significant public health issue	174:206	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	1	17	theme	public	188:193	arg1	persistence					136:146	The worldwide persistence	122:146	The worldwide persistence of infectious diseases	122:169	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	2	18	attach	present	261:267	arg2	ingredients					249:259	immunomodulatory ingredients	232:259	immunomodulatory ingredients present in natural products, such as ginseng,	232:305	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	2	18	attach	present	261:267	arg1	ginseng					298:304	ginseng	298:304	ginseng	298:304	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	2	18	attach	present	261:267	arg1	products					280:287	natural products	272:287	natural products	272:287	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	9	19	theme	different	1636:1644	arg1	compositions					1655:1666	different chemical compositions	1636:1666	different chemical compositions	1636:1666	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	8	20	theme	protein	1323:1329	arg1	p38					1354:1356	p38	1354:1356	p38	1354:1356	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	20	theme	protein	1323:1329	arg1	ERK					1340:1342	ERK	1340:1342	ERK	1340:1342	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	20	theme	protein	1323:1329	arg1	kinases					1331:1337	mitogen-activated protein kinases	1305:1337	mitogen-activated protein kinases (ERK, JNK, and p38)	1305:1357	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	20	theme	protein	1323:1329	arg1	JNK					1345:1347	JNK	1345:1347	JNK	1345:1347	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	1	21	theme	health	195:200	arg1	issue					202:206	a significant public health issue	174:206	a significant public health issue	174:206	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	1	21	theme	health	195:200	arg1	persistence					136:146	The worldwide persistence	122:146	The worldwide persistence of infectious diseases	122:169	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	3	22	theme	polysaccharides	402:416	arg1	types					393:397	three different types	377:397	three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	377:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	9	23	theme	chemical	1646:1653	arg1	compositions					1655:1666	different chemical compositions	1636:1666	different chemical compositions	1636:1666	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	2	24	theme	treatment	339:347	arg1	options					349:355	new treatment options	335:355	new treatment options	335:355	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	9	25	from	changes	1585:1591	arg1	processing					1613:1622	heat processing	1608:1622	heat processing	1608:1622	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	6	26	with	Treatment	876:884	arg1	P-HPG					905:909	P-HPG	905:909	P-HPG	905:909	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	6	26	with	Treatment	876:884	arg1	P-RG					897:900	P-RG	897:900	P-RG	897:900	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	6	26	with	Treatment	876:884	arg1	P-WG					891:894	P-WG	891:894	P-WG	891:894	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	5	27	theme	processing	815:824	arg1	temperature					826:836	processing temperature	815:836	processing temperature	815:836	Chemical analysis indicated that the content of carbohydrates (total sugar) increased with processing temperature, while that of uronic acid decreased.
36874224	6	28	theme	tumor	965:969	arg1	alpha					987:991	tumor necrosis factor alpha	965:991	tumor necrosis factor alpha (TNF-α)	965:999	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	6	28	theme	tumor	965:969	arg1	TNF-α					994:998	TNF-α	994:998	TNF-α	994:998	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	2	29	theme	new	335:337	arg1	options					349:355	new treatment options	335:355	new treatment options	335:355	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	4	30	theme	uronic	672:677	arg1	acid					679:682	uronic acid	672:682	uronic acid	672:682	Carbohydrates were the main components of all three polysaccharide types, while uronic acid and protein levels were relatively low.
36874224	0	31	theme	activation	31:40	arg1	Characterization					0:15	Characterization	0:15	Characterization of macrophage activation after treatment with polysaccharides from ginseng	0:90	Characterization of macrophage activation after treatment with polysaccharides from ginseng according to heat processing.
36874224	3	32	from	ginseng	476:482	arg1	polysaccharides					402:416	polysaccharides	402:416	polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	402:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	32	from	ginseng	476:482	arg1	types					393:397	three different types	377:397	three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	377:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	6	33	theme	IL	1018:1019	arg1	levels					1024:1029	tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels	965:1029	tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages	965:1054	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	2	34	from	products	280:287	arg1	present					261:267	present	261:267	present	261:267	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	8	35	dep	kinases	1331:1337	arg1	p38					1354:1356	p38	1354:1356	p38	1354:1356	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	35	dep	kinases	1331:1337	arg1	ERK					1340:1342	ERK	1340:1342	ERK	1340:1342	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	35	dep	kinases	1331:1337	arg1	kinases					1331:1337	mitogen-activated protein kinases	1305:1337	mitogen-activated protein kinases (ERK, JNK, and p38)	1305:1357	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	35	dep	kinases	1331:1337	arg1	JNK					1345:1347	JNK	1345:1347	JNK	1345:1347	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	0	36	theme	macrophage	20:29	arg1	activation					31:40	macrophage activation	20:40	macrophage activation after treatment with polysaccharides from ginseng	20:90	Characterization of macrophage activation after treatment with polysaccharides from ginseng according to heat processing.
36874224	9	37	theme	immune-enhancing	1672:1687	arg1	activities					1689:1698	immune-enhancing activities	1672:1698	immune-enhancing activities	1672:1698	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	3	38	theme	RAW	562:564	arg1	macrophages					579:589	RAW 264.7 murine macrophages	562:589	RAW 264.7 murine macrophages	562:589	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	39	theme	heat-processed	453:466	arg1	ginseng					476:482	heat-processed (P-HPG) ginseng	453:482	heat-processed (P-HPG) ginseng	453:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	5	40	theme	total	787:791	arg1	carbohydrates					772:784	carbohydrates	772:784	carbohydrates (total sugar)	772:798	Chemical analysis indicated that the content of carbohydrates (total sugar) increased with processing temperature, while that of uronic acid decreased.
36874224	5	40	theme	total	787:791	arg1	sugar					793:797	total sugar	787:797	total sugar	787:797	Chemical analysis indicated that the content of carbohydrates (total sugar) increased with processing temperature, while that of uronic acid decreased.
36874224	8	41	theme	pathways	1284:1291	arg1	Analysis					1248:1255	Analysis	1248:1255	Analysis of intracellular signaling pathways	1248:1291	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	4	42	theme	types	659:663	arg1	Carbohydrates					592:604	Carbohydrates	592:604	Carbohydrates	592:604	Carbohydrates were the main components of all three polysaccharide types, while uronic acid and protein levels were relatively low.
36874224	4	42	theme	types	659:663	arg1	components					620:629	the main components	611:629	the main components of all three polysaccharide types	611:663	Carbohydrates were the main components of all three polysaccharide types, while uronic acid and protein levels were relatively low.
36874224	2	43	theme	natural	272:278	arg1	ginseng					298:304	ginseng	298:304	ginseng	298:304	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	2	43	theme	natural	272:278	arg1	products					280:287	natural products	272:287	natural products	272:287	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	3	44	theme	murine	572:577	arg1	macrophages					579:589	RAW 264.7 murine macrophages	562:589	RAW 264.7 murine macrophages	562:589	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	4	45	theme	protein	688:694	arg1	levels					696:701	protein levels	688:701	protein levels	688:701	Carbohydrates were the main components of all three polysaccharide types, while uronic acid and protein levels were relatively low.
36874224	5	46	theme	Chemical	724:731	arg1	analysis					733:740	Chemical analysis	724:740	Chemical analysis	724:740	Chemical analysis indicated that the content of carbohydrates (total sugar) increased with processing temperature, while that of uronic acid decreased.
36874224	0	47	from	ginseng	84:90	arg1	polysaccharides					63:77	polysaccharides	63:77	polysaccharides from ginseng	63:90	Characterization of macrophage activation after treatment with polysaccharides from ginseng according to heat processing.
36874224	7	48	theme	NO	1157:1158	arg1	synthase					1160:1167	inducible NO synthase	1147:1167	inducible NO synthase	1147:1167	The expression of inducible NO synthase, which affects NO secretion, was highest in the macrophages treated with P-WG.
36874224	4	49	theme	polysaccharide	644:657	arg1	types					659:663	all three polysaccharide types	634:663	all three polysaccharide types	634:663	Carbohydrates were the main components of all three polysaccharide types, while uronic acid and protein levels were relatively low.
36874224	8	50	theme	mitogen-activated	1305:1321	arg1	p38					1354:1356	p38	1354:1356	p38	1354:1356	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	50	theme	mitogen-activated	1305:1321	arg1	ERK					1340:1342	ERK	1340:1342	ERK	1340:1342	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	50	theme	mitogen-activated	1305:1321	arg1	kinases					1331:1337	mitogen-activated protein kinases	1305:1337	mitogen-activated protein kinases (ERK, JNK, and p38)	1305:1357	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	8	50	theme	mitogen-activated	1305:1321	arg1	JNK					1345:1347	JNK	1345:1347	JNK	1345:1347	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	2	51	theme	present	261:267	arg1	ingredients					249:259	immunomodulatory ingredients	232:259	immunomodulatory ingredients present in natural products, such as ginseng,	232:305	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	1	52	theme	worldwide	126:134	arg1	issue					202:206	a significant public health issue	174:206	a significant public health issue	174:206	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	1	52	theme	worldwide	126:134	arg1	persistence					136:146	The worldwide persistence	122:146	The worldwide persistence of infectious diseases	122:169	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	6	53	theme	nitric	922:927	arg1	NO					936:937	NO	936:937	NO	936:937	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	6	53	theme	nitric	922:927	arg1	oxide					929:933	nitric oxide	922:933	nitric oxide (NO) production	922:949	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	2	54	theme	immunomodulatory	232:247	arg1	ingredients					249:259	immunomodulatory ingredients	232:259	immunomodulatory ingredients present in natural products, such as ginseng,	232:305	Consequently, studying immunomodulatory ingredients present in natural products, such as ginseng, is important for developing new treatment options.
36874224	9	55	dep	processing	1613:1622	arg1	response					1596:1603	response	1596:1603	response	1596:1603	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	6	56	theme	highest	1079:1085	arg1	activity					1087:1094	the highest activity	1075:1094	the highest activity among the three polysaccharides	1075:1126	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	6	57	theme	oxide	929:933	arg1	production					940:949	nitric oxide (NO) production	922:949	nitric oxide (NO) production	922:949	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	9	58	theme	different	1575:1583	arg1	changes					1585:1591	different changes	1575:1591	different changes in response to heat processing	1575:1622	Collectively, these results suggest that the polysaccharides isolated from ginseng undergo different changes in response to heat processing and display different chemical compositions and immune-enhancing activities.
36874224	8	59	theme	NF-kB	1363:1367	arg1	p65					1369:1371	NF-kB p65	1363:1371	NF-kB p65	1363:1371	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	4	60	theme	main	615:618	arg1	Carbohydrates					592:604	Carbohydrates	592:604	Carbohydrates	592:604	Carbohydrates were the main components of all three polysaccharide types, while uronic acid and protein levels were relatively low.
36874224	4	60	theme	main	615:618	arg1	components					620:629	the main components	611:629	the main components of all three polysaccharide types	611:663	Carbohydrates were the main components of all three polysaccharide types, while uronic acid and protein levels were relatively low.
36874224	8	61	theme	signaling	1274:1282	arg1	pathways					1284:1291	intracellular signaling pathways	1260:1291	intracellular signaling pathways	1260:1291	Analysis of intracellular signaling pathways showed that mitogen-activated protein kinases (ERK, JNK, and p38) and NF-kB p65 were strongly phosphorylated by P-WG in macrophages but were only moderately phosphorylated by P-RG and P-HPG.
36874224	1	62	theme	infectious	151:160	arg1	diseases					162:169	infectious diseases	151:169	infectious diseases	151:169	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	5	63	theme	uronic	853:858	arg1	acid					860:863	uronic acid	853:863	uronic acid	853:863	Chemical analysis indicated that the content of carbohydrates (total sugar) increased with processing temperature, while that of uronic acid decreased.
36874224	3	64	from	red	437:439	arg1	polysaccharides					402:416	polysaccharides	402:416	polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	402:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	64	from	red	437:439	arg1	types					393:397	three different types	377:397	three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	377:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	1	65	theme	diseases	162:169	arg1	issue					202:206	a significant public health issue	174:206	a significant public health issue	174:206	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	1	65	theme	diseases	162:169	arg1	persistence					136:146	The worldwide persistence	122:146	The worldwide persistence of infectious diseases	122:169	The worldwide persistence of infectious diseases is a significant public health issue.
36874224	6	66	theme	RAW	1034:1036	arg1	macrophages					1044:1054	RAW 264.7 macrophages	1034:1054	RAW 264.7 macrophages	1034:1054	Treatment with P-WG, P-RG or P-HPG stimulated nitric oxide (NO) production and increased tumor necrosis factor alpha (TNF-α) and interleukin (IL)-6 levels in RAW 264.7 macrophages, with P-WG showing the highest activity among the three polysaccharides.
36874224	3	67	from	white	423:427	arg1	polysaccharides					402:416	polysaccharides	402:416	polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	402:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	67	from	white	423:427	arg1	types					393:397	three different types	377:397	three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	377:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	0	68	with	treatment	48:56	arg1	polysaccharides					63:77	polysaccharides	63:77	polysaccharides from ginseng	63:90	Characterization of macrophage activation after treatment with polysaccharides from ginseng according to heat processing.
36874224	3	69	theme	chemical	503:510	arg1	properties					512:521	their chemical properties	497:521	their chemical properties	497:521	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36874224	3	70	theme	different	383:391	arg1	types					393:397	three different types	377:397	three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng	377:482	Here, we extracted three different types of polysaccharides from white (P-WG), red (P-RG), and heat-processed (P-HPG) ginseng and analyzed their chemical properties and immunostimulatory activity against RAW 264.7 murine macrophages.
36359990	5	0	theme	tea	1001:1003	arg1	samples					1020:1026	the tea polysaccharide samples	997:1026	the tea polysaccharide samples extracted from different pile-fermentation stages	997:1076	According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1.
36359990	1	1	theme	mass	208:211	arg1	UHPLC-Q-TOF-MS/MS					227:243	UHPLC-Q-TOF-MS/MS	227:243	UHPLC-Q-TOF-MS/MS	227:243	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	1	theme	mass	208:211	arg1	spectrometry					213:224	flight tandem mass spectrometry	194:224	flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	194:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	5	2	theme	different	1043:1051	arg1	stages					1071:1076	different pile-fermentation stages	1043:1076	different pile-fermentation stages	1043:1076	According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1.
36359990	3	3	from	mponents	554:561	arg1	polysaccharides					570:584	tea polysaccharides	566:584	tea polysaccharides	566:584	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	1	4	theme	spectrometry	213:224	arg1	chromatography-quadrupole-time					160:189	Ultra-high performance liquid chromatography-quadrupole-time	130:189	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	130:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	0	5	theme	Tea	125:127	arg1	Pile-Fermentation					89:105	the Pile-Fermentation	85:105	the Pile-Fermentation of Post-Fermented Tea	85:127	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	8	6	theme	polysaccharides	1559:1573	arg1	function					1543:1550	the function	1539:1550	the function of tea polysaccharides	1539:1573	The study will further help to reveal the function of tea polysaccharides and promote their practical application as a functional food.
36359990	2	7	theme	free	394:397	arg1	radical					399:405	the free radical	390:405	the free radical scavenging ability of tea polysaccharides	390:447	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	7	8	theme	polysaccharides	1434:1448	arg1	composition					1401:1411	composition	1401:1411	composition	1401:1411	Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages.
36359990	7	8	theme	polysaccharides	1434:1448	arg1	abundance					1421:1429	the abundance	1417:1429	the abundance of polysaccharides	1417:1448	Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages.
36359990	5	9	theme	polysaccharide	1005:1018	arg1	samples					1020:1026	the tea polysaccharide samples	997:1026	the tea polysaccharide samples extracted from different pile-fermentation stages	997:1076	According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1.
36359990	6	10	theme	DPPH	1252:1255	arg1	radical					1262:1268	the DPPH free radical	1248:1268	the DPPH free radical	1248:1268	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	1	11	theme	dynamic	309:315	arg1	changes					317:323	the dynamic changes	305:323	the dynamic changes in the physicochemical indexes of tea samples	305:369	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	0	12	theme	Post-Fermented	110:123	arg1	Tea					125:127	Post-Fermented Tea	110:127	Post-Fermented Tea	110:127	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	6	13	theme	tea	1292:1294	arg1	polysaccharides					1296:1310	tea polysaccharides	1292:1310	tea polysaccharides	1292:1310	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	2	14	theme	radical	399:405	arg1	ability					418:424	the free radical scavenging ability	390:424	the free radical scavenging ability of tea polysaccharides	390:447	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	6	15	theme	spectra	1235:1241	arg1	ability					1281:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability	1187:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides	1187:1310	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	3	16	theme	saccharide	540:549	arg1	mponents					554:561	23 saccharide co mponents	537:561	23 saccharide co mponents in tea polysaccharides	537:584	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	6	17	theme	component	1218:1226	arg1	radical					1262:1268	the DPPH free radical	1248:1268	the DPPH free radical	1248:1268	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	6	17	theme	component	1218:1226	arg1	yield					1191:1195	yield	1191:1195	yield	1191:1195	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	6	17	theme	component	1218:1226	arg1	content					1202:1208	the content	1198:1208	the content of each component	1198:1226	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	6	17	theme	component	1218:1226	arg1	spectra					1235:1241	FT-IR spectra	1229:1241	FT-IR spectra	1229:1241	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	5	18	theme	polysaccharide	949:962	arg1	composition					964:974	polysaccharide composition	949:974	polysaccharide composition	949:974	According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1.
36359990	4	19	theme	oligosaccharides	737:752	arg1	abundance					724:732	The abundance	720:732	The abundance of oligosaccharides	720:752	The abundance of oligosaccharides decreased gradually, while monosaccharides, and derivatives of monosaccharides and oligosaccharides increased gradually with the development of pile-fermentation.
36359990	7	20	theme	pile-fermentation	1475:1491	arg1	stages					1493:1498	different pile-fermentation stages	1465:1498	different pile-fermentation stages	1465:1498	Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages.
36359990	7	21	theme	Correlation	1313:1323	arg1	analysis					1325:1332	Correlation analysis	1313:1332	Correlation analysis	1313:1332	Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages.
36359990	2	22	theme	polysaccharides	433:447	arg1	spectra					378:384	FT-IR spectra	372:384	FT-IR spectra	372:384	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	2	22	theme	polysaccharides	433:447	arg1	ability					418:424	the free radical scavenging ability	390:424	the free radical scavenging ability of tea polysaccharides	390:447	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	6	23	theme	scavenging	1270:1279	arg1	ability					1281:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability	1187:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides	1187:1310	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	4	24	theme	monosaccharides	817:831	arg1	monosaccharides					781:795	monosaccharides	781:795	monosaccharides	781:795	The abundance of oligosaccharides decreased gradually, while monosaccharides, and derivatives of monosaccharides and oligosaccharides increased gradually with the development of pile-fermentation.
36359990	4	24	theme	monosaccharides	817:831	arg1	derivatives					802:812	derivatives	802:812	derivatives	802:812	The abundance of oligosaccharides decreased gradually, while monosaccharides, and derivatives of monosaccharides and oligosaccharides increased gradually with the development of pile-fermentation.
36359990	5	25	from	differences	934:944	arg1	composition					964:974	polysaccharide composition	949:974	polysaccharide composition	949:974	According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1.
36359990	5	25	from	differences	934:944	arg1	abundance					986:994	their abundance	980:994	their abundance	980:994	According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1.
36359990	2	26	theme	tea	429:431	arg1	polysaccharides					433:447	tea polysaccharides	429:447	tea polysaccharides	429:447	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	0	27	theme	Dynamic	0:6	arg1	Analysis					8:15	Dynamic Analysis	0:15	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition	0:76	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	6	28	theme	radical	1262:1268	arg1	ability					1281:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability	1187:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides	1187:1310	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	7	29	theme	different	1465:1473	arg1	stages					1493:1498	different pile-fermentation stages	1465:1498	different pile-fermentation stages	1465:1498	Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages.
36359990	0	30	theme	Physicochemical	20:34	arg1	Properties					36:45	Physicochemical Properties	20:45	Physicochemical Properties	20:45	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	2	31	theme	scavenging	407:416	arg1	ability					418:424	the free radical scavenging ability	390:424	the free radical scavenging ability of tea polysaccharides	390:447	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	7	32	from	changes	1390:1396	arg1	composition					1401:1411	composition	1401:1411	composition	1401:1411	Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages.
36359990	7	32	from	changes	1390:1396	arg1	abundance					1421:1429	the abundance	1417:1429	the abundance of polysaccharides	1417:1448	Correlation analysis showed that microorganisms were directly related to the changes in composition and the abundance of polysaccharides extracted from different pile-fermentation stages.
36359990	4	33	theme	oligosaccharides	837:852	arg1	monosaccharides					781:795	monosaccharides	781:795	monosaccharides	781:795	The abundance of oligosaccharides decreased gradually, while monosaccharides, and derivatives of monosaccharides and oligosaccharides increased gradually with the development of pile-fermentation.
36359990	4	33	theme	oligosaccharides	837:852	arg1	derivatives					802:812	derivatives	802:812	derivatives	802:812	The abundance of oligosaccharides decreased gradually, while monosaccharides, and derivatives of monosaccharides and oligosaccharides increased gradually with the development of pile-fermentation.
36359990	2	34	theme	post-fermented	478:491	arg1	tea					493:495	post-fermented tea	478:495	post-fermented tea	478:495	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	2	35	theme	tea	493:495	arg1	pile-fermentation					457:473	pile-fermentation	457:473	pile-fermentation of post-fermented tea	457:495	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	1	36	theme	physicochemical	332:346	arg1	indexes					348:354	the physicochemical indexes	328:354	the physicochemical indexes of tea samples	328:369	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	37	from	changes	317:323	arg1	indexes					348:354	the physicochemical indexes	328:354	the physicochemical indexes of tea samples	328:369	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	3	38	theme	oligosaccharides	702:717	arg1	oligosaccharides					643:658	5 oligosaccharides	641:658	5 oligosaccharides	641:658	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	3	38	theme	oligosaccharides	702:717	arg1	derivatives					667:677	6 derivatives	665:677	6 derivatives	665:677	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	3	38	theme	oligosaccharides	702:717	arg1	monosaccharides					624:638	11 monosaccharides	621:638	11 monosaccharides	621:638	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	1	39	theme	Ultra-high	130:139	arg1	chromatography-quadrupole-time					160:189	Ultra-high performance liquid chromatography-quadrupole-time	130:189	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	130:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	3	40	theme	tea	566:568	arg1	polysaccharides					570:584	tea polysaccharides	566:584	tea polysaccharides	566:584	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	3	41	theme	co	551:552	arg1	mponents					554:561	23 saccharide co mponents	537:561	23 saccharide co mponents in tea polysaccharides	537:584	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	8	42	theme	practical	1593:1601	arg1	application					1603:1613	their practical application	1587:1613	their practical application as a functional food	1587:1634	The study will further help to reveal the function of tea polysaccharides and promote their practical application as a functional food.
36359990	0	43	theme	Properties	36:45	arg1	Analysis					8:15	Dynamic Analysis	0:15	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition	0:76	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	3	44	theme	monosaccharides	682:696	arg1	oligosaccharides					643:658	5 oligosaccharides	641:658	5 oligosaccharides	641:658	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	3	44	theme	monosaccharides	682:696	arg1	derivatives					667:677	6 derivatives	665:677	6 derivatives	665:677	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	3	44	theme	monosaccharides	682:696	arg1	monosaccharides					624:638	11 monosaccharides	621:638	11 monosaccharides	621:638	The results showed that 23 saccharide co mponents in tea polysaccharides were identified: these belonged to 11 monosaccharides, 5 oligosaccharides, and 6 derivatives of monosaccharides and oligosaccharides.
36359990	0	45	theme	Composition	66:76	arg1	Analysis					8:15	Dynamic Analysis	0:15	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition	0:76	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	1	46	theme	tea	359:361	arg1	samples					363:369	tea samples	359:369	tea samples	359:369	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	8	47	theme	tea	1555:1557	arg1	polysaccharides					1559:1573	tea polysaccharides	1555:1573	tea polysaccharides	1555:1573	The study will further help to reveal the function of tea polysaccharides and promote their practical application as a functional food.
36359990	6	48	theme	FT-IR	1229:1233	arg1	spectra					1235:1241	FT-IR spectra	1229:1241	FT-IR spectra	1229:1241	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	1	49	theme	performance	141:151	arg1	chromatography-quadrupole-time					160:189	Ultra-high performance liquid chromatography-quadrupole-time	130:189	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	130:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	0	50	theme	Polysaccharide	51:64	arg1	Composition					66:76	Polysaccharide Composition	51:76	Polysaccharide Composition	51:76	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	1	51	theme	samples	363:369	arg1	indexes					348:354	the physicochemical indexes	328:354	the physicochemical indexes of tea samples	328:369	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	52	used	used	250:253	arg2	chromatography-quadrupole-time					160:189	Ultra-high performance liquid chromatography-quadrupole-time	130:189	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	130:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	53	theme	liquid	153:158	arg1	chromatography-quadrupole-time					160:189	Ultra-high performance liquid chromatography-quadrupole-time	130:189	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	130:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	54	from	diversity	268:276	arg1	indexes					348:354	the physicochemical indexes	328:354	the physicochemical indexes of tea samples	328:369	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	8	55	theme	functional	1620:1629	arg1	food					1631:1634	a functional food	1618:1634	a functional food	1618:1634	The study will further help to reveal the function of tea polysaccharides and promote their practical application as a functional food.
36359990	5	56	theme	pile-fermentation	1053:1069	arg1	stages					1071:1076	different pile-fermentation stages	1043:1076	different pile-fermentation stages	1043:1076	According to the differences in polysaccharide composition and their abundance, the tea polysaccharide samples extracted from different pile-fermentation stages could be clearly classed into three groups, W-0, W-1~W-4 and W-5~C-1.
36359990	6	57	theme	free	1257:1260	arg1	radical					1262:1268	the DPPH free radical	1248:1268	the DPPH free radical	1248:1268	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	6	58	theme	pile-fermentation	1152:1168	arg1	process					1170:1176	The pile-fermentation process	1148:1176	The pile-fermentation process	1148:1176	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	6	59	theme	polysaccharides	1296:1310	arg1	ability					1281:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability	1187:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides	1187:1310	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	2	60	theme	FT-IR	372:376	arg1	spectra					378:384	FT-IR spectra	372:384	FT-IR spectra	372:384	FT-IR spectra and the free radical scavenging ability of tea polysaccharides, during pile-fermentation of post-fermented tea, were analyzed.
36359990	0	61	dep	Analysis	8:15	arg1	Pile-Fermentation					89:105	the Pile-Fermentation	85:105	the Pile-Fermentation of Post-Fermented Tea	85:127	Dynamic Analysis of Physicochemical Properties and Polysaccharide Composition during the Pile-Fermentation of Post-Fermented Tea.
36359990	6	62	theme	yield	1191:1195	arg1	ability					1281:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability	1187:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides	1187:1310	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	1	63	theme	flight	194:199	arg1	UHPLC-Q-TOF-MS/MS					227:243	UHPLC-Q-TOF-MS/MS	227:243	UHPLC-Q-TOF-MS/MS	227:243	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	63	theme	flight	194:199	arg1	spectrometry					213:224	flight tandem mass spectrometry	194:224	flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	194:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	6	64	theme	content	1202:1208	arg1	ability					1281:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability	1187:1287	the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides	1187:1310	The pile-fermentation process affected the yield, the content of each component, FT-IR spectra, and the DPPH free radical scavenging ability of tea polysaccharides.
36359990	1	65	theme	tea	281:283	arg1	polysaccharides					285:299	tea polysaccharides	281:299	tea polysaccharides	281:299	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	4	66	theme	pile-fermentation	898:914	arg1	development					883:893	the development	879:893	the development of pile-fermentation	879:914	The abundance of oligosaccharides decreased gradually, while monosaccharides, and derivatives of monosaccharides and oligosaccharides increased gradually with the development of pile-fermentation.
36359990	1	67	theme	tandem	201:206	arg1	UHPLC-Q-TOF-MS/MS					227:243	UHPLC-Q-TOF-MS/MS	227:243	UHPLC-Q-TOF-MS/MS	227:243	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	67	theme	tandem	201:206	arg1	spectrometry					213:224	flight tandem mass spectrometry	194:224	flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS)	194:244	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	68	theme	polysaccharides	285:299	arg1	diversity					268:276	the diversity	264:276	the diversity of tea polysaccharides	264:299	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36359990	1	68	theme	polysaccharides	285:299	arg1	changes					317:323	the dynamic changes	305:323	the dynamic changes in the physicochemical indexes of tea samples	305:369	Ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) was used to study the diversity of tea polysaccharides and the dynamic changes in the physicochemical indexes of tea samples.
36778094	4	0	from	effects	499:505	arg1	microbiota					525:534	the gut microbiota	517:534	the gut microbiota	517:534	In addition, 16S rRNA sequencing were used to assess the effects of FVP on the gut microbiota.
36778094	1	1	theme	many	167:170	arg1	activities					183:192	many biological activities	167:192	many biological activities	167:192	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	10	2	theme	available	1272:1280	arg1	material					1263:1270	supplementary material	1249:1270	supplementary material available at 10.1007/s10068-022-01192-y	1249:1310	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	8	3	theme	acids	1085:1089	arg1	synthesis					1054:1062	synthesis	1054:1062	synthesis of unsaturated fatty acids	1054:1089	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	8	3	theme	acids	1085:1089	arg1	glycerophospholipid					1015:1033	glycerophospholipid	1015:1033	glycerophospholipid	1015:1033	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	8	3	theme	acids	1085:1089	arg1	acid					1045:1048	linoleic acid	1036:1048	linoleic acid	1036:1048	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	1	4	theme	biological	172:181	arg1	activities					183:192	many biological activities	167:192	many biological activities	167:192	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	0	5	from	Effects	0:6	arg1	metabolism					82:91	metabolism	82:91	metabolism	82:91	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism in vitro fermentation.
36778094	0	5	from	Effects	0:6	arg1	composition					66:76	gut microbiota composition	51:76	gut microbiota composition	51:76	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism in vitro fermentation.
36778094	2	6	from	influence	312:320	arg1	microflora					335:344	human gut microflora composition and metabolites	325:372	microflora	335:344	Here, we explored the composition of FVP, their influence on human gut microflora composition and metabolites.
36778094	2	6	from	influence	312:320	arg1	metabolites					362:372	metabolites	362:372	metabolites	362:372	Here, we explored the composition of FVP, their influence on human gut microflora composition and metabolites.
36778094	10	7	from	10.1007/s10068-022-01192-y	1285:1310	arg1	available					1272:1280	available	1272:1280	available	1272:1280	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	10	8	theme	supplementary	1249:1261	arg1	material					1263:1270	supplementary material	1249:1270	supplementary material available at 10.1007/s10068-022-01192-y	1249:1310	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	5	9	theme	metabolomics	595:606	arg1	approaches					608:617	untargeted metabolomics approaches	584:617	untargeted metabolomics approaches	584:617	The metabolic profiles were investigated using untargeted metabolomics approaches in the LC-MS platform.
36778094	2	10	dep	microflora	335:344	arg1	composition					346:356	composition	346:356	composition	346:356	Here, we explored the composition of FVP, their influence on human gut microflora composition and metabolites.
36778094	7	11	theme	Bacteroidaceae	825:838	arg1	abundances					780:789	the relative abundances	767:789	the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae	767:838	FVP is shown to increase the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae and remarkably decrease the numbers of genera Lachnospiraceae coupled with Enterococcaceae.
36778094	2	12	theme	gut	331:333	arg1	microflora					335:344	human gut microflora composition and metabolites	325:372	microflora	335:344	Here, we explored the composition of FVP, their influence on human gut microflora composition and metabolites.
36778094	8	13	theme	unsaturated	1067:1077	arg1	acids					1085:1089	unsaturated fatty acids	1067:1089	unsaturated fatty acids	1067:1089	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	0	14	theme	gut	51:53	arg1	composition					66:76	gut microbiota composition	51:76	gut microbiota composition	51:76	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism in vitro fermentation.
36778094	5	15	theme	metabolic	541:549	arg1	profiles					551:558	The metabolic profiles	537:558	The metabolic profiles	537:558	The metabolic profiles were investigated using untargeted metabolomics approaches in the LC-MS platform.
36778094	5	16	theme	LC-MS	626:630	arg1	platform					632:639	the LC-MS platform	622:639	the LC-MS platform	622:639	The metabolic profiles were investigated using untargeted metabolomics approaches in the LC-MS platform.
36778094	4	17	theme	rRNA	459:462	arg1	sequencing					464:473	16S rRNA sequencing	455:473	16S rRNA sequencing	455:473	In addition, 16S rRNA sequencing were used to assess the effects of FVP on the gut microbiota.
36778094	3	18	theme	human	419:423	arg1	inoculums					431:439	human fecal inoculums	419:439	human fecal inoculums	419:439	FVP were used to vitro fermentation through human fecal inoculums.
36778094	7	19	theme	relative	771:778	arg1	abundances					780:789	the relative abundances	767:789	the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae	767:838	FVP is shown to increase the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae and remarkably decrease the numbers of genera Lachnospiraceae coupled with Enterococcaceae.
36778094	8	20	theme	fatty	1079:1083	arg1	acids					1085:1089	unsaturated fatty acids	1067:1089	unsaturated fatty acids	1067:1089	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	9	21	theme	biological	1108:1117	arg1	function					1128:1135	biological activity function	1108:1135	biological activity function	1108:1135	FVP may exhibit biological activity function by regulating gut microflora composition and metabolites.
36778094	3	22	theme	fecal	425:429	arg1	inoculums					431:439	human fecal inoculums	419:439	human fecal inoculums	419:439	FVP were used to vitro fermentation through human fecal inoculums.
36778094	0	23	theme	velutipes	22:30	arg1	polysaccharides					32:46	Flammulina velutipes polysaccharides	11:46	Flammulina velutipes polysaccharides	11:46	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism in vitro fermentation.
36778094	4	24	theme	gut	521:523	arg1	microbiota					525:534	the gut microbiota	517:534	the gut microbiota	517:534	In addition, 16S rRNA sequencing were used to assess the effects of FVP on the gut microbiota.
36778094	8	25	theme	glycerophospholipid	1015:1033	arg1	metabolism					1001:1010	the metabolism	997:1010	the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids	997:1089	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	2	26	theme	FVP	301:303	arg1	composition					286:296	the composition	282:296	the composition of FVP, their influence on human gut microflora composition and metabolites	282:372	Here, we explored the composition of FVP, their influence on human gut microflora composition and metabolites.
36778094	9	27	theme	activity	1119:1126	arg1	function					1128:1135	biological activity function	1108:1135	biological activity function	1108:1135	FVP may exhibit biological activity function by regulating gut microflora composition and metabolites.
36778094	0	28	theme	Flammulina	11:20	arg1	polysaccharides					32:46	Flammulina velutipes polysaccharides	11:46	Flammulina velutipes polysaccharides	11:46	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism in vitro fermentation.
36778094	1	29	theme	Flammulina	116:125	arg1	FVP					154:156	FVP	154:156	FVP	154:156	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	1	29	theme	Flammulina	116:125	arg1	polysaccharides					137:151	Flammulina velutipes polysaccharides	116:151	Flammulina velutipes polysaccharides (FVP)	116:157	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	3	30	used	used	384:387	arg2	FVP					375:377	FVP	375:377	FVP	375:377	FVP were used to vitro fermentation through human fecal inoculums.
36778094	7	31	theme	Bifidobacteriaceae	794:811	arg1	abundances					780:789	the relative abundances	767:789	the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae	767:838	FVP is shown to increase the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae and remarkably decrease the numbers of genera Lachnospiraceae coupled with Enterococcaceae.
36778094	1	32	theme	gut	214:216	arg1	microflora					218:227	gut microflora	214:227	gut microflora	214:227	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	4	33	used	used	480:483	arg2	sequencing					464:473	16S rRNA sequencing	455:473	16S rRNA sequencing	455:473	In addition, 16S rRNA sequencing were used to assess the effects of FVP on the gut microbiota.
36778094	4	34	theme	FVP	510:512	arg1	effects					499:505	the effects	495:505	the effects of FVP on the gut microbiota	495:534	In addition, 16S rRNA sequencing were used to assess the effects of FVP on the gut microbiota.
36778094	0	35	theme	polysaccharides	32:46	arg1	Effects					0:6	Effects	0:6	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism	0:91	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism in vitro fermentation.
36778094	4	36	theme	16S	455:457	arg1	sequencing					464:473	16S rRNA sequencing	455:473	16S rRNA sequencing	455:473	In addition, 16S rRNA sequencing were used to assess the effects of FVP on the gut microbiota.
36778094	0	37	theme	microbiota	55:64	arg1	composition					66:76	gut microbiota composition	51:76	gut microbiota composition	51:76	Effects of Flammulina velutipes polysaccharides on gut microbiota composition and metabolism in vitro fermentation.
36778094	10	38	theme	Supplementary	1195:1207	arg1	Information					1209:1219	Supplementary Information	1195:1219	Supplementary Information The online version	1195:1238	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	9	39	theme	gut	1151:1153	arg1	microflora					1155:1164	gut microflora composition and metabolites	1151:1192	microflora	1155:1164	FVP may exhibit biological activity function by regulating gut microflora composition and metabolites.
36778094	1	40	theme	velutipes	127:135	arg1	FVP					154:156	FVP	154:156	FVP	154:156	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	1	40	theme	velutipes	127:135	arg1	polysaccharides					137:151	Flammulina velutipes polysaccharides	116:151	Flammulina velutipes polysaccharides (FVP)	116:157	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	3	41	theme	vitro	392:396	arg1	fermentation					398:409	vitro fermentation	392:409	vitro fermentation through human fecal inoculums	392:439	FVP were used to vitro fermentation through human fecal inoculums.
36778094	10	42	dep	Information	1209:1219	arg1	version					1232:1238	The online version	1221:1238	Supplementary Information The online version	1195:1238	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	1	43	from	effects	203:209	arg1	metabolism					233:242	metabolism	233:242	metabolism	233:242	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	1	43	from	effects	203:209	arg1	microflora					218:227	gut microflora	214:227	gut microflora	214:227	Flammulina velutipes polysaccharides (FVP) exhibit many biological activities, but the effects on gut microflora and metabolism were still unclear.
36778094	2	44	theme	human	325:329	arg1	microflora					335:344	human gut microflora composition and metabolites	325:372	microflora	335:344	Here, we explored the composition of FVP, their influence on human gut microflora composition and metabolites.
36778094	7	45	theme	Lachnospiraceae	886:900	arg1	numbers					868:874	the numbers	864:874	the numbers of genera Lachnospiraceae coupled with Enterococcaceae	864:929	FVP is shown to increase the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae and remarkably decrease the numbers of genera Lachnospiraceae coupled with Enterococcaceae.
36778094	10	46	contain	contains	1240:1247	arg1	Information					1209:1219	Supplementary Information	1195:1219	Supplementary Information The online version	1195:1238	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	10	46	contain	contains	1240:1247	arg2	material					1263:1270	supplementary material	1249:1270	supplementary material available at 10.1007/s10068-022-01192-y	1249:1310	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	9	47	dep	microflora	1155:1164	arg1	composition					1166:1176	composition	1166:1176	composition	1166:1176	FVP may exhibit biological activity function by regulating gut microflora composition and metabolites.
36778094	5	48	theme	untargeted	584:593	arg1	approaches					608:617	untargeted metabolomics approaches	584:617	untargeted metabolomics approaches	584:617	The metabolic profiles were investigated using untargeted metabolomics approaches in the LC-MS platform.
36778094	10	49	theme	online	1225:1230	arg1	version					1232:1238	The online version	1221:1238	Supplementary Information The online version	1195:1238	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01192-y.
36778094	8	50	theme	differential	936:947	arg1	metabolites					949:959	The differential metabolites	932:959	The differential metabolites	932:959	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	7	51	theme	genera	879:884	arg1	Lachnospiraceae					886:900	genera Lachnospiraceae	879:900	genera Lachnospiraceae coupled with Enterococcaceae	879:929	FVP is shown to increase the relative abundances of Bifidobacteriaceae, as well as Bacteroidaceae and remarkably decrease the numbers of genera Lachnospiraceae coupled with Enterococcaceae.
36778094	8	52	theme	linoleic	1036:1043	arg1	glycerophospholipid					1015:1033	glycerophospholipid	1015:1033	glycerophospholipid	1015:1033	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36778094	8	52	theme	linoleic	1036:1043	arg1	acid					1045:1048	linoleic acid	1036:1048	linoleic acid	1036:1048	The differential metabolites were identified and mainly involved the metabolism of glycerophospholipid, linoleic acid and synthesis of unsaturated fatty acids.
36900432	0	0	theme	flagelliforme	82:94	arg1	Production					61:70	the Polysaccharide Production	42:70	the Polysaccharide Production of Nostoc flagelliforme	42:94	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	7	1	theme	polysaccharides	1459:1473	arg1	accumulation					1443:1454	the accumulation	1439:1454	the accumulation of polysaccharides	1439:1473	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	2	theme	oxide	1267:1271	arg1	levels					1273:1278	the nitric oxide levels	1256:1278	the nitric oxide levels	1256:1278	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	0	3	theme	Nostoc	75:80	arg1	flagelliforme					82:94	Nostoc flagelliforme	75:94	Nostoc flagelliforme	75:94	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	7	4	from	effects	1182:1188	arg1	levels					1273:1278	the nitric oxide levels	1256:1278	the nitric oxide levels	1256:1278	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	4	from	effects	1182:1188	arg1	yield					1299:1303	polysaccharide yield	1284:1303	polysaccharide yield of N. flagelliforme	1284:1323	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	4	5	theme	2.16	894:897	arg1	×					887:887	2.06 × 103	882:891	2.06 × 103	882:891	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	4	5	theme	2.16	894:897	arg1	×					899:899	2.16 × 103 and 2.04 × 103 kDa	894:922	×	899:899	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	2	6	theme	jasmonic	358:365	arg1	acid					367:370	jasmonic acid	358:370	jasmonic acid	358:370	The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in N. flagelliforme significantly, by more than 20%.
36900432	7	7	theme	nitric	1234:1239	arg1	oxide					1241:1245	nitric oxide	1234:1245	nitric oxide donor	1234:1251	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	8	8	theme	secondary	1551:1559	arg1	metabolites					1561:1571	secondary metabolites	1551:1571	secondary metabolites	1551:1571	These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels.
36900432	7	9	theme	donor	1247:1251	arg1	effects					1182:1188	the effects	1178:1188	the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme	1178:1323	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	10	theme	oxide	1241:1245	arg1	donor					1247:1251	nitric oxide donor	1234:1251	nitric oxide donor	1234:1251	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	4	11	theme	sugar	816:820	arg1	contents					838:845	the total sugar and uronic acid contents	806:845	the total sugar and uronic acid contents	806:845	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	3	12	theme	jasmonic	580:587	arg1	polysaccharide					602:615	jasmonic acid-capsule polysaccharide	580:615	jasmonic acid-capsule polysaccharide	580:615	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	3	12	theme	jasmonic	580:587	arg1	polysaccharides					481:495	Three polysaccharides	475:495	Three polysaccharides	475:495	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	7	13	theme	scavenger	1220:1228	arg1	effects					1182:1188	the effects	1178:1188	the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme	1178:1323	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	14	theme	N.	1308:1309	arg1	flagelliforme					1311:1323	N. flagelliforme	1308:1323	N. flagelliforme	1308:1323	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	6	15	theme	salicylic	1077:1085	arg1	acid					1087:1090	salicylic acid	1077:1090	salicylic acid	1077:1090	It was revealed that the salicylic acid and jasmonic acid significantly increased the level of nitric oxide.
36900432	4	16	theme	acid	833:836	arg1	contents					838:845	the total sugar and uronic acid contents	806:845	the total sugar and uronic acid contents	806:845	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	7	17	theme	intracellular	1366:1378	arg1	levels					1393:1398	intracellular nitric oxide levels	1366:1398	intracellular nitric oxide levels	1366:1398	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	2	18	theme	polysaccharides	406:420	arg1	accumulation					386:397	the accumulation	382:397	the accumulation of the polysaccharides in N. flagelliforme	382:440	The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in N. flagelliforme significantly, by more than 20%.
36900432	8	19	theme	intracellular	1591:1603	arg1	levels					1618:1623	the intracellular nitric oxide levels	1587:1623	the intracellular nitric oxide levels	1587:1623	These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels.
36900432	4	20	theme	×	887:887	arg1	weights					871:877	average molecular weights	853:877	average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa	853:922	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	4	21	theme	uronic	826:831	arg1	contents					838:845	the total sugar and uronic acid contents	806:845	the total sugar and uronic acid contents	806:845	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	5	22	from	difference	1016:1025	arg1	activity					1042:1049	antioxidant activity	1030:1049	antioxidant activity	1030:1049	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	1	23	theme	polysaccharide	175:188	arg1	production					190:199	the polysaccharide production	171:199	the polysaccharide production of Nostoc flagelliforme	171:223	To significantly improve the polysaccharide production of Nostoc flagelliforme, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation.
36900432	7	24	theme	nitric	1207:1212	arg1	oxide					1214:1218	the exogenous nitric oxide	1193:1218	the exogenous nitric oxide scavenger	1193:1228	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	1	25	from	effects	275:281	arg1	accumulation					301:312	polysaccharide accumulation	286:312	polysaccharide accumulation	286:312	To significantly improve the polysaccharide production of Nostoc flagelliforme, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation.
36900432	5	26	theme	antioxidant	1030:1040	arg1	activity					1042:1049	antioxidant activity	1030:1049	antioxidant activity	1030:1049	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	5	27	theme	similar	954:960	arg1	Fourier					962:968	similar Fourier transform infrared spectra and no significant difference in antioxidant activity	954:1049	Fourier	962:968	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	6	28	theme	jasmonic	1096:1103	arg1	acid					1105:1108	jasmonic acid	1096:1108	jasmonic acid	1096:1108	It was revealed that the salicylic acid and jasmonic acid significantly increased the level of nitric oxide.
36900432	0	29	theme	Salicylic	0:8	arg1	Acid					10:13	Salicylic Acid	0:13	Salicylic Acid	0:13	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	8	30	theme	oxide	1612:1616	arg1	levels					1618:1623	the intracellular nitric oxide levels	1587:1623	the intracellular nitric oxide levels	1587:1623	These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels.
36900432	0	31	theme	Jasmonic	19:26	arg1	Acid					28:31	Jasmonic Acid	19:31	Jasmonic Acid	19:31	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	4	32	theme	average	853:859	arg1	weights					871:877	average molecular weights	853:877	average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa	853:922	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	0	33	theme	Intracellular	122:134	arg1	Level					139:143	the Intracellular NO Level	118:143	the Intracellular NO Level	118:143	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	3	34	theme	acid-capsule	589:600	arg1	polysaccharide					602:615	jasmonic acid-capsule polysaccharide	580:615	jasmonic acid-capsule polysaccharide	580:615	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	3	34	theme	acid-capsule	589:600	arg1	polysaccharides					481:495	Three polysaccharides	475:495	Three polysaccharides	475:495	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	1	35	theme	Nostoc	204:209	arg1	flagelliforme					211:223	Nostoc flagelliforme	204:223	Nostoc flagelliforme	204:223	To significantly improve the polysaccharide production of Nostoc flagelliforme, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation.
36900432	7	36	theme	exogenous	1197:1205	arg1	oxide					1214:1218	the exogenous nitric oxide	1193:1218	the exogenous nitric oxide scavenger	1193:1228	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	1	37	theme	polysaccharide	286:299	arg1	accumulation					301:312	polysaccharide accumulation	286:312	polysaccharide accumulation	286:312	To significantly improve the polysaccharide production of Nostoc flagelliforme, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation.
36900432	3	38	theme	acid	692:695	arg1	conditions					724:733	normal, salicylic acid, and jasmonic acid culture conditions	674:733	normal, salicylic acid, and jasmonic acid culture conditions	674:733	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	5	39	theme	infrared	980:987	arg1	spectra					989:995	infrared spectra	980:995	infrared spectra	980:995	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	1	40	theme	flagelliforme	211:223	arg1	production					190:199	the polysaccharide production	171:199	the polysaccharide production of Nostoc flagelliforme	171:223	To significantly improve the polysaccharide production of Nostoc flagelliforme, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation.
36900432	7	41	theme	flagelliforme	1311:1323	arg1	levels					1273:1278	the nitric oxide levels	1256:1278	the nitric oxide levels	1256:1278	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	41	theme	flagelliforme	1311:1323	arg1	yield					1299:1303	polysaccharide yield	1284:1303	polysaccharide yield of N. flagelliforme	1284:1323	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	0	42	theme	Level	139:143	arg1	Regulation					104:113	the Regulation	100:113	the Regulation of the Intracellular NO Level	100:143	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	7	43	theme	oxide	1214:1218	arg1	scavenger					1220:1228	the exogenous nitric oxide scavenger	1193:1228	the exogenous nitric oxide scavenger	1193:1228	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	2	44	theme	N.	425:426	arg1	flagelliforme					428:440	N. flagelliforme	425:440	N. flagelliforme	425:440	The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in N. flagelliforme significantly, by more than 20%.
36900432	0	45	theme	NO	136:137	arg1	Level					139:143	the Intracellular NO Level	118:143	the Intracellular NO Level	118:143	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	8	46	theme	theoretical	1501:1511	arg1	foundation					1513:1522	a theoretical foundation	1499:1522	a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels	1499:1623	These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels.
36900432	4	47	theme	molecular	861:869	arg1	weights					871:877	average molecular weights	853:877	average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa	853:922	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	0	48	theme	Polysaccharide	46:59	arg1	Production					61:70	the Polysaccharide Production	42:70	the Polysaccharide Production of Nostoc flagelliforme	42:94	Salicylic Acid and Jasmonic Acid Increase the Polysaccharide Production of Nostoc flagelliforme via the Regulation of the Intracellular NO Level.
36900432	3	49	theme	salicylic	682:690	arg1	acid					692:695	salicylic acid	682:695	salicylic acid	682:695	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	2	50	from	accumulation	386:397	arg1	flagelliforme					428:440	N. flagelliforme	425:440	N. flagelliforme	425:440	The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in N. flagelliforme significantly, by more than 20%.
36900432	4	51	theme	chemical	756:763	arg1	compositions					765:776	Their chemical compositions	750:776	Their chemical compositions	750:776	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	5	52	dep	Fourier	962:968	arg1	transform					970:978	transform	970:978	transform	970:978	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	5	52	dep	Fourier	962:968	arg1	spectra					989:995	infrared spectra	980:995	infrared spectra	980:995	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	4	53	theme	×	914:914	arg1	kDa					920:922	2.16 × 103 and 2.04 × 103 kDa	894:922	kDa	920:922	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	4	53	theme	×	914:914	arg1	×					887:887	2.06 × 103	882:891	2.06 × 103	882:891	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	3	54	theme	acid	711:714	arg1	conditions					724:733	normal, salicylic acid, and jasmonic acid culture conditions	674:733	normal, salicylic acid, and jasmonic acid culture conditions	674:733	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	3	55	theme	control-capsule	505:519	arg1	polysaccharides					481:495	Three polysaccharides	475:495	Three polysaccharides	475:495	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	3	55	theme	control-capsule	505:519	arg1	polysaccharide					521:534	control-capsule polysaccharide	505:534	control-capsule polysaccharide	505:534	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	7	56	theme	polysaccharide	1284:1297	arg1	yield					1299:1303	polysaccharide yield	1284:1303	polysaccharide yield of N. flagelliforme	1284:1323	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	3	57	theme	culture	716:722	arg1	conditions					724:733	normal, salicylic acid, and jasmonic acid culture conditions	674:733	normal, salicylic acid, and jasmonic acid culture conditions	674:733	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	7	58	theme	oxide	1387:1391	arg1	levels					1393:1398	intracellular nitric oxide levels	1366:1398	intracellular nitric oxide levels	1366:1398	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	59	theme	important	1412:1420	arg1	factor					1422:1427	an important factor	1409:1427	an important factor promoting the accumulation of polysaccharides	1409:1473	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	59	theme	important	1412:1420	arg1	increase					1354:1361	the increase	1350:1361	the increase in intracellular nitric oxide levels	1350:1398	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	2	60	theme	salicylic	339:347	arg1	acid					349:352	salicylic acid	339:352	salicylic acid	339:352	The results showed that salicylic acid and jasmonic acid increased the accumulation of the polysaccharides in N. flagelliforme significantly, by more than 20%.
36900432	4	61	theme	total	810:814	arg1	sugar					816:820	the total sugar	806:820	the total sugar	806:820	Their chemical compositions slightly differed regarding the total sugar and uronic acid contents, with average molecular weights of 2.06 × 103, 2.16 × 103 and 2.04 × 103 kDa, respectively.
36900432	5	62	theme	significant	1004:1014	arg1	difference					1016:1025	no significant difference	1001:1025	no significant difference in antioxidant activity	1001:1049	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	3	63	theme	normal	674:679	arg1	acid					692:695	salicylic acid	682:695	salicylic acid	682:695	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	6	64	theme	nitric	1147:1152	arg1	oxide					1154:1158	nitric oxide	1147:1158	nitric oxide	1147:1158	It was revealed that the salicylic acid and jasmonic acid significantly increased the level of nitric oxide.
36900432	6	65	theme	oxide	1154:1158	arg1	level					1138:1142	the level	1134:1142	the level of nitric oxide	1134:1158	It was revealed that the salicylic acid and jasmonic acid significantly increased the level of nitric oxide.
36900432	8	66	theme	metabolites	1561:1571	arg1	yield					1542:1546	the yield	1538:1546	the yield of secondary metabolites	1538:1571	These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels.
36900432	3	67	theme	jasmonic	702:709	arg1	acid					711:714	jasmonic acid	702:714	jasmonic acid	702:714	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	3	68	theme	N.	651:652	arg1	flagelliforme					654:666	N. flagelliforme	651:666	N. flagelliforme	651:666	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	8	69	theme	nitric	1605:1610	arg1	oxide					1612:1616	nitric oxide	1605:1616	the intracellular nitric oxide levels	1587:1623	These findings provide a theoretical foundation for enhancing the yield of secondary metabolites by regulating the intracellular nitric oxide levels.
36900432	3	70	theme	salicylic	537:545	arg1	polysaccharide					560:573	salicylic acid-capsule polysaccharide	537:573	salicylic acid-capsule polysaccharide	537:573	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	3	70	theme	salicylic	537:545	arg1	polysaccharides					481:495	Three polysaccharides	475:495	Three polysaccharides	475:495	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	5	71	from	Fourier	962:968	arg1	activity					1042:1049	antioxidant activity	1030:1049	antioxidant activity	1030:1049	They presented similar Fourier transform infrared spectra and no significant difference in antioxidant activity.
36900432	7	72	theme	nitric	1260:1265	arg1	oxide					1267:1271	the nitric oxide	1256:1271	the nitric oxide levels	1256:1278	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	73	theme	nitric	1380:1385	arg1	oxide					1387:1391	nitric oxide	1380:1391	intracellular nitric oxide levels	1366:1398	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	7	74	from	increase	1354:1361	arg1	levels					1393:1398	intracellular nitric oxide levels	1366:1398	intracellular nitric oxide levels	1366:1398	By investigating the effects of the exogenous nitric oxide scavenger and nitric oxide donor on the nitric oxide levels and polysaccharide yield of N. flagelliforme, the results showed that the increase in intracellular nitric oxide levels might be an important factor promoting the accumulation of polysaccharides.
36900432	3	75	theme	acid-capsule	547:558	arg1	polysaccharide					560:573	salicylic acid-capsule polysaccharide	537:573	salicylic acid-capsule polysaccharide	537:573	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	3	75	theme	acid-capsule	547:558	arg1	polysaccharides					481:495	Three polysaccharides	475:495	Three polysaccharides	475:495	Three polysaccharides, namely control-capsule polysaccharide, salicylic acid-capsule polysaccharide, and jasmonic acid-capsule polysaccharide, were extracted and purified from N. flagelliforme under normal, salicylic acid, and jasmonic acid culture conditions, respectively.
36900432	1	76	theme	chemicals	240:248	arg1	total					228:232	a total	226:232	a total of 12 chemicals	226:248	To significantly improve the polysaccharide production of Nostoc flagelliforme, a total of 12 chemicals were evaluated for their effects on polysaccharide accumulation.
34989366	0	0	theme	peonies	75:81	arg1	coats					61:65	the seed coats	52:65	the seed coats of tree peonies	52:81	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	0	0	theme	peonies	75:81	arg1	activities					106:115	their hypoglycemic activities	87:115	their hypoglycemic activities in vitro/vivo	87:129	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	4	1	theme	resveratrol	501:511	arg1	derivatives					513:523	resveratrol derivatives	501:523	resveratrol derivatives	501:523	Seven polyphenolics were separated and prepared, mainly belonging to resveratrol derivatives.
34989366	5	2	theme	resveratrol	645:655	arg1	trimers					657:663	the resveratrol trimers	641:663	the resveratrol trimers	641:663	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	8	3	theme	in	1155:1156	arg1	tests					1171:1175	in vitro enzyme tests	1155:1175	in vitro enzyme tests	1155:1175	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	1	4	theme	well-known	149:158	arg1	plants					188:193	well-known horticultural and medicinal plants	149:193	well-known horticultural and medicinal plants	149:193	Tree peonies are well-known horticultural and medicinal plants.
34989366	1	4	theme	well-known	149:158	arg1	peonies					137:143	Tree peonies	132:143	Tree peonies	132:143	Tree peonies are well-known horticultural and medicinal plants.
34989366	8	5	dep	in	1155:1156	arg1	vitro					1158:1162	vitro	1158:1162	vitro	1158:1162	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	0	6	theme	tree	70:73	arg1	peonies					75:81	tree peonies	70:81	tree peonies	70:81	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	0	7	from	analysis	13:20	arg1	coats					61:65	the seed coats	52:65	the seed coats of tree peonies	52:81	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	0	7	from	analysis	13:20	arg1	activities					106:115	their hypoglycemic activities	87:115	their hypoglycemic activities in vitro/vivo	87:129	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	5	8	theme	trimers	657:663	arg1	higher					695:700	higher	695:700	higher	695:700	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	5	8	theme	trimers	657:663	arg1	contents					629:636	the contents	625:636	the contents of the resveratrol trimers and dimers	625:674	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	5	9	theme	Paeonia	604:610	arg1	species					612:618	eight Paeonia species	598:618	eight Paeonia species	598:618	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	1	10	theme	horticultural	160:172	arg1	plants					188:193	well-known horticultural and medicinal plants	149:193	well-known horticultural and medicinal plants	149:193	Tree peonies are well-known horticultural and medicinal plants.
34989366	1	10	theme	horticultural	160:172	arg1	peonies					137:143	Tree peonies	132:143	Tree peonies	132:143	Tree peonies are well-known horticultural and medicinal plants.
34989366	8	11	theme	IC50	1275:1278	arg1	values					1280:1285	the IC50 values	1271:1285	the IC50 values	1271:1285	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	5	12	theme	Paeonia	723:729	arg1	ostii					731:735	Paeonia ostii	723:735	Paeonia ostii	723:735	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	8	13	theme	therapeutic	1381:1391	arg1	agents					1403:1408	therapeutic potential agents	1381:1408	therapeutic potential agents	1381:1408	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	4	14	dep	separated	457:465	arg1	belonging					488:496	belonging	488:496	belonging to resveratrol derivatives	488:523	Seven polyphenolics were separated and prepared, mainly belonging to resveratrol derivatives.
34989366	5	15	theme	ostii	731:735	arg1	coats					714:718	the seed coats	705:718	the seed coats of Paeonia ostii	705:735	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	6	16	theme	composition	898:908	arg1	patterns					910:917	the composition patterns	894:917	the composition patterns	894:917	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	5	17	theme	dimers	669:674	arg1	higher					695:700	higher	695:700	higher	695:700	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	5	17	theme	dimers	669:674	arg1	contents					629:636	the contents	625:636	the contents of the resveratrol trimers and dimers	625:674	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	6	18	theme	chemometric	807:817	arg1	analysis					819:826	chemometric analysis	807:826	chemometric analysis	807:826	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	2	19	theme	great	271:275	arg1	attention					277:285	great attention	271:285	great attention	271:285	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	8	20	theme	enzyme	1164:1169	arg1	tests					1171:1175	in vitro enzyme tests	1155:1175	in vitro enzyme tests	1155:1175	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	0	21	theme	hypoglycemic	93:104	arg1	activities					106:115	their hypoglycemic activities	87:115	their hypoglycemic activities in vitro/vivo	87:129	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	8	22	theme	potential	1393:1401	arg1	agents					1403:1408	therapeutic potential agents	1381:1408	therapeutic potential agents	1381:1408	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	5	23	theme	polyphenolic	571:582	arg1	content					584:590	the seed coat polyphenolic content	557:590	the seed coat polyphenolic content	557:590	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	0	24	theme	Quantitative	0:11	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.	0:130	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	1	25	theme	medicinal	178:186	arg1	plants					188:193	well-known horticultural and medicinal plants	149:193	well-known horticultural and medicinal plants	149:193	Tree peonies are well-known horticultural and medicinal plants.
34989366	1	25	theme	medicinal	178:186	arg1	peonies					137:143	Tree peonies	132:143	Tree peonies	132:143	Tree peonies are well-known horticultural and medicinal plants.
34989366	3	26	theme	tree	376:379	arg1	coats					392:396	tree peony seed coats	376:396	tree peony seed coats	376:396	In this study, the phytochemicals isolated from tree peony seed coats were systematically investigated.
34989366	7	27	theme	diabetic/normal	1120:1134	arg1	mice					1136:1139	diabetic/normal mice	1120:1139	diabetic/normal mice	1120:1139	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	6	28	theme	fingerprint	775:785	arg1	characteristics					787:801	the HPLC fingerprint characteristics	766:801	the HPLC fingerprint characteristics	766:801	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	3	29	theme	peony	381:385	arg1	coats					392:396	tree peony seed coats	376:396	tree peony seed coats	376:396	In this study, the phytochemicals isolated from tree peony seed coats were systematically investigated.
34989366	6	30	theme	Paeonia	858:864	arg1	plants					866:871	Paeonia plants	858:871	Paeonia plants	858:871	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	6	31	theme	HPLC	770:773	arg1	characteristics					787:801	the HPLC fingerprint characteristics	766:801	the HPLC fingerprint characteristics	766:801	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	0	32	theme	resveratrol	25:35	arg1	derivatives					37:47	resveratrol derivatives	25:47	resveratrol derivatives	25:47	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	7	33	theme	glucose	1109:1115	arg1	levels					1080:1085	the starch-mediated levels	1060:1085	the starch-mediated levels of postprandial blood glucose in diabetic/normal mice	1060:1139	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	8	34	theme	diabetes	1432:1439	arg1	mellitus					1441:1448	diabetes mellitus	1432:1448	diabetes mellitus	1432:1448	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	7	35	theme	characteristic	967:980	arg1	trans-ε-viniferin					1014:1030	trans-ε-viniferin	1014:1030	trans-ε-viniferin	1014:1030	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	7	35	theme	characteristic	967:980	arg1	vateriferol					998:1008	vateriferol	998:1008	vateriferol	998:1008	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	7	35	theme	characteristic	967:980	arg1	phytochemicals					982:995	the characteristic phytochemicals	963:995	the characteristic phytochemicals (vateriferol and trans-ε-viniferin)	963:1031	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	5	36	theme	seed	709:712	arg1	coats					714:718	the seed coats	705:718	the seed coats of Paeonia ostii	705:735	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	7	37	theme	postprandial	1090:1101	arg1	glucose					1109:1115	postprandial blood glucose	1090:1115	postprandial blood glucose	1090:1115	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	2	38	theme	woody	230:234	arg1	crops					240:244	as emerging woody oil crops	218:244	as emerging woody oil crops	218:244	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	2	38	theme	woody	230:234	arg1	seeds					211:215	The tree peony seeds	196:215	The tree peony seeds	196:215	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	7	39	dep	phytochemicals	982:995	arg1	trans-ε-viniferin					1014:1030	trans-ε-viniferin	1014:1030	trans-ε-viniferin	1014:1030	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	7	39	dep	phytochemicals	982:995	arg1	vateriferol					998:1008	vateriferol	998:1008	vateriferol	998:1008	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	7	39	dep	phytochemicals	982:995	arg1	phytochemicals					982:995	the characteristic phytochemicals	963:995	the characteristic phytochemicals (vateriferol and trans-ε-viniferin)	963:1031	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	0	40	theme	derivatives	37:47	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.	0:130	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	7	41	theme	blood	1103:1107	arg1	glucose					1109:1115	postprandial blood glucose	1090:1115	postprandial blood glucose	1090:1115	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	2	42	theme	emerging	221:228	arg1	crops					240:244	as emerging woody oil crops	218:244	as emerging woody oil crops	218:244	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	2	42	theme	emerging	221:228	arg1	seeds					211:215	The tree peony seeds	196:215	The tree peony seeds	196:215	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	5	43	theme	other	742:746	arg1	species					748:754	other species	742:754	other species	742:754	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	0	44	theme	seed	56:59	arg1	coats					61:65	the seed coats	52:65	the seed coats of tree peonies	52:81	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	0	45	from	coats	61:65	arg1	vitro/vivo					120:129	vitro/vivo	120:129	vitro/vivo	120:129	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	6	46	theme	constituents	939:950	arg1	contents					923:930	contents	923:930	contents of the constituents	923:950	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	6	46	theme	constituents	939:950	arg1	patterns					910:917	the composition patterns	894:917	the composition patterns	894:917	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	0	47	from	activities	106:115	arg1	vitro/vivo					120:129	vitro/vivo	120:129	vitro/vivo	120:129	Quantitative analysis of resveratrol derivatives in the seed coats of tree peonies and their hypoglycemic activities in vitro/vivo.
34989366	5	48	theme	seed	561:564	arg1	content					584:590	the seed coat polyphenolic content	557:590	the seed coat polyphenolic content	557:590	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	2	49	theme	peony	205:209	arg1	crops					240:244	as emerging woody oil crops	218:244	as emerging woody oil crops	218:244	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	2	49	theme	peony	205:209	arg1	seeds					211:215	The tree peony seeds	196:215	The tree peony seeds	196:215	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	5	50	theme	coat	566:569	arg1	content					584:590	the seed coat polyphenolic content	557:590	the seed coat polyphenolic content	557:590	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	8	51	theme	μM	1304:1305	arg1	values					1280:1285	the IC50 values	1271:1285	the IC50 values	1271:1285	In addition, in vitro enzyme tests showed that the two compounds could effectively and competitively inhibit α-glucosidase, with the IC50 values of 3.01 and 7.75 μM, respectively, indicating that vateriferol and trans-ε-viniferin could be therapeutic potential agents for hyperglycemia and diabetes mellitus.
34989366	7	52	theme	starch-mediated	1064:1078	arg1	levels					1080:1085	the starch-mediated levels	1060:1085	the starch-mediated levels of postprandial blood glucose in diabetic/normal mice	1060:1139	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	2	53	theme	tree	200:203	arg1	crops					240:244	as emerging woody oil crops	218:244	as emerging woody oil crops	218:244	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	2	53	theme	tree	200:203	arg1	seeds					211:215	The tree peony seeds	196:215	The tree peony seeds	196:215	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	1	54	theme	Tree	132:135	arg1	plants					188:193	well-known horticultural and medicinal plants	149:193	well-known horticultural and medicinal plants	149:193	Tree peonies are well-known horticultural and medicinal plants.
34989366	1	54	theme	Tree	132:135	arg1	peonies					137:143	Tree peonies	132:143	Tree peonies	132:143	Tree peonies are well-known horticultural and medicinal plants.
34989366	3	55	attach	isolated	362:369	arg2	phytochemicals					347:360	the phytochemicals	343:360	the phytochemicals isolated from tree peony seed coats	343:396	In this study, the phytochemicals isolated from tree peony seed coats were systematically investigated.
34989366	3	55	attach	isolated	362:369	arg1	coats					392:396	tree peony seed coats	376:396	tree peony seed coats	376:396	In this study, the phytochemicals isolated from tree peony seed coats were systematically investigated.
34989366	6	56	theme	clear	831:835	arg1	discrimination					837:850	a clear discrimination	829:850	a clear discrimination among Paeonia plants	829:871	Based on the HPLC fingerprint characteristics and chemometric analysis, a clear discrimination among Paeonia plants was found, including the composition patterns and contents of the constituents.
34989366	5	57	from	variation	544:552	arg1	content					584:590	the seed coat polyphenolic content	557:590	the seed coat polyphenolic content	557:590	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
34989366	7	58	from	levels	1080:1085	arg1	mice					1136:1139	diabetic/normal mice	1120:1139	diabetic/normal mice	1120:1139	Moreover, the characteristic phytochemicals (vateriferol and trans-ε-viniferin) could significantly reduce the starch-mediated levels of postprandial blood glucose in diabetic/normal mice.
34989366	3	59	theme	seed	387:390	arg1	coats					392:396	tree peony seed coats	376:396	tree peony seed coats	376:396	In this study, the phytochemicals isolated from tree peony seed coats were systematically investigated.
34989366	2	60	theme	oil	236:238	arg1	crops					240:244	as emerging woody oil crops	218:244	as emerging woody oil crops	218:244	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	2	60	theme	oil	236:238	arg1	seeds					211:215	The tree peony seeds	196:215	The tree peony seeds	196:215	The tree peony seeds, as emerging woody oil crops, recently have attracted great attention for their metabolites and bioactivities.
34989366	5	61	theme	great	538:542	arg1	variation					544:552	a great variation	536:552	a great variation in the seed coat polyphenolic content among eight Paeonia species	536:618	There was a great variation in the seed coat polyphenolic content among eight Paeonia species, and the contents of the resveratrol trimers and dimers were significantly higher in the seed coats of Paeonia ostii than other species.
36494098	2	0	theme	-interacting	246:257	arg1	kinase					267:272	Calcineurin B-like (CBL)-interacting protein kinase 9	222:274	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9)	222:282	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	2	0	theme	-interacting	246:257	arg1	CIPK9					277:281	CIPK9	277:281	CIPK9	277:281	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	4	1	theme	bnacipk9	752:759	arg1	lines					761:765	bnacipk9 lines	752:765	bnacipk9 lines	752:765	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	7	2	from	effects	1618:1624	arg1	accumulation					1667:1678	seed oil accumulation	1658:1678	seed oil accumulation	1658:1678	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	4	3	theme	bnacipk9	615:622	arg1	lines					624:628	gene-silenced and bnacipk9 lines	597:628	gene-silenced and bnacipk9 lines	597:628	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	8	4	theme	glucose	1775:1781	arg1	flux					1783:1786	glucose flux	1775:1786	glucose flux	1775:1786	Altogether, our results strongly suggest a role of CIPK9 in mediating the interaction between glucose flux and ABA hormone signaling to regulate seed oil metabolism in rapeseed.
36494098	2	5	theme	CBL	242:244	arg1	kinase					267:272	Calcineurin B-like (CBL)-interacting protein kinase 9	222:274	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9)	222:282	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	2	5	theme	CBL	242:244	arg1	CIPK9					277:281	CIPK9	277:281	CIPK9	277:281	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	1	6	theme	crop	149:152	arg1	worldwide					154:162	an important oil crop worldwide	132:162	an important oil crop worldwide	132:162	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	1	6	theme	crop	149:152	arg1	Rapeseed					105:112	Rapeseed	105:112	Rapeseed (Brassica napus)	105:129	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	4	7	theme	increased	827:835	arg1	levels					837:842	increased levels	827:842	increased levels of monounsaturated fatty acids	827:873	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	7	8	theme	seed	1658:1661	arg1	accumulation					1667:1678	seed oil accumulation	1658:1678	seed oil accumulation	1658:1678	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	8	9	theme	hormone	1796:1802	arg1	signaling					1804:1812	ABA hormone signaling	1792:1812	ABA hormone signaling	1792:1812	Altogether, our results strongly suggest a role of CIPK9 in mediating the interaction between glucose flux and ABA hormone signaling to regulate seed oil metabolism in rapeseed.
36494098	3	10	theme	rapeseeds	531:539	arg1	metabolism					517:526	seed oil metabolism	508:526	seed oil metabolism of rapeseeds	508:539	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	6	11	theme	expression	1414:1423	arg1	levels					1425:1430	the expression levels	1410:1430	the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds	1410:1500	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	1	12	theme	large	174:178	arg1	lipids					191:196	lipids	191:196	lipids	191:196	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	1	12	theme	large	174:178	arg1	amounts					180:186	large amounts	174:186	large amounts of lipids	174:196	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	4	13	theme	gene-silenced	597:609	arg1	lines					624:628	gene-silenced and bnacipk9 lines	597:628	gene-silenced and bnacipk9 lines	597:628	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	14	theme	WT	1007:1008	arg1	lines					1010:1014	WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds	1007:1278	WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds	1007:1278	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	4	15	from	%	747:747	arg1	lines					761:765	bnacipk9 lines	752:765	bnacipk9 lines	752:765	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	15	from	%	747:747	arg1	lines					785:789	gene-silenced lines	771:789	gene-silenced lines	771:789	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	16	theme	fatty	675:679	arg1	compositions					686:697	fatty acid compositions	675:697	fatty acid compositions	675:697	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	8	17	theme	seed	1826:1829	arg1	metabolism					1835:1844	seed oil metabolism	1826:1844	seed oil metabolism	1826:1844	Altogether, our results strongly suggest a role of CIPK9 in mediating the interaction between glucose flux and ABA hormone signaling to regulate seed oil metabolism in rapeseed.
36494098	5	18	theme	content	962:968	arg1	analyses					970:977	hormone and glucose content analyses	942:977	analyses	970:977	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	7	19	theme	glucose	1637:1643	arg1	signaling					1645:1653	ABA and glucose signaling	1629:1653	ABA and glucose signaling	1629:1653	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	2	20	theme	B-like	234:239	arg1	kinase					267:272	Calcineurin B-like (CBL)-interacting protein kinase 9	222:274	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9)	222:282	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	2	20	theme	B-like	234:239	arg1	CIPK9					277:281	CIPK9	277:281	CIPK9	277:281	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	7	21	theme	ABA	1629:1631	arg1	signaling					1645:1653	ABA and glucose signaling	1629:1653	ABA and glucose signaling	1629:1653	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	3	22	theme	oil	513:515	arg1	metabolism					517:526	seed oil metabolism	508:526	seed oil metabolism of rapeseeds	508:539	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	4	23	theme	different	648:656	arg1	contents					662:669	substantially different oil contents	634:669	substantially different oil contents	634:669	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	24	theme	higher	1196:1201	arg1	ratio					1221:1225	a higher hexose-to-sucrose ratio	1194:1225	a higher hexose-to-sucrose ratio	1194:1225	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	5	25	theme	significant	1042:1052	arg1	differences					1054:1064	significant differences	1042:1064	significant differences	1042:1064	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	6	26	theme	ABA	1465:1467	arg1	pathways					1480:1487	ABA regulating pathways	1465:1487	ABA regulating pathways	1465:1487	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	4	27	theme	polyunsaturated	899:913	arg1	acids					921:925	polyunsaturated fatty acids	899:925	polyunsaturated fatty acids	899:925	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	28	theme	sucrose	1160:1166	arg1	contents					1168:1175	glucose and sucrose contents	1148:1175	glucose and sucrose contents	1148:1175	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	0	29	theme	seed	72:75	arg1	metabolism					81:90	seed oil metabolism	72:90	seed oil metabolism	72:90	Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed.
36494098	1	30	theme	important	135:143	arg1	worldwide					154:162	an important oil crop worldwide	132:162	an important oil crop worldwide	132:162	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	1	30	theme	important	135:143	arg1	Rapeseed					105:112	Rapeseed	105:112	Rapeseed (Brassica napus)	105:129	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	4	31	theme	fatty	863:867	arg1	acids					869:873	monounsaturated fatty acids	847:873	monounsaturated fatty acids	847:873	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	1	32	dep	Brassica	115:122	arg1	napus					124:128	Brassica napus	115:128	Brassica napus	115:128	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	7	33	theme	developing	1588:1597	arg1	seeds					1599:1603	developing seeds	1588:1603	developing seeds	1588:1603	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	3	34	theme	RNA	383:385	arg1	interference					387:398	RNA interference biotechnology and loss-of-function mutant bnacipk9	383:449	interference	387:398	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	4	35	theme	seed	700:703	arg1	content					709:715	seed oil content	700:715	seed oil content	700:715	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	36	theme	maturation	1251:1260	arg1	seeds					1274:1278	late maturation development seeds	1246:1278	late maturation development seeds	1246:1278	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	0	37	theme	Kinase	0:5	arg1	CIPK9					7:11	Kinase CIPK9	0:11	Kinase CIPK9	0:11	Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed.
36494098	5	38	theme	acid	1099:1102	arg1	levels					1128:1133	indoleacetic acid and abscisic acid (ABA) levels	1086:1133	indoleacetic acid and abscisic acid (ABA) levels	1086:1133	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	3	39	theme	loss-of-function	418:433	arg1	bnacipk9					442:449	loss-of-function mutant bnacipk9	418:449	loss-of-function mutant bnacipk9	418:449	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	4	40	from	%	737:737	arg1	lines					761:765	bnacipk9 lines	752:765	bnacipk9 lines	752:765	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	40	from	%	737:737	arg1	lines					785:789	gene-silenced lines	771:789	gene-silenced lines	771:789	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	41	theme	abscisic	1108:1115	arg1	ABA					1123:1125	ABA	1123:1125	ABA	1123:1125	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	5	41	theme	abscisic	1108:1115	arg1	acid					1117:1120	abscisic acid	1108:1120	abscisic acid (ABA)	1108:1126	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	7	42	theme	exogenous	1551:1559	arg1	ABA					1561:1563	exogenous ABA	1551:1563	exogenous ABA	1551:1563	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	4	43	theme	acids	921:925	arg1	levels					889:894	decreased levels	879:894	decreased levels of polyunsaturated fatty acids	879:925	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	43	theme	acids	921:925	arg1	levels					837:842	increased levels	827:842	increased levels of monounsaturated fatty acids	827:873	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	44	theme	bnacipk9	1230:1237	arg1	mid					1239:1241	bnacipk9 mid	1230:1241	bnacipk9 mid to late maturation development seeds	1230:1278	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	5	45	theme	bnacipk9	1070:1077	arg1	seeds					1079:1083	bnacipk9 seeds	1070:1083	bnacipk9 seeds	1070:1083	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	3	46	theme	gene-silenced	355:367	arg1	lines					369:373	gene-silenced lines	355:373	gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9	355:449	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	5	47	theme	hormone	942:948	arg1	analyses					970:977	hormone and glucose content analyses	942:977	analyses	970:977	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	0	48	theme	acid	45:48	arg1	signaling					50:58	glucose and abscisic acid signaling	24:58	glucose and abscisic acid signaling	24:58	Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed.
36494098	5	49	theme	glucose	954:960	arg1	analyses					970:977	hormone and glucose content analyses	942:977	analyses	970:977	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	4	50	contain	had	630:632	arg2	compositions					686:697	fatty acid compositions	675:697	fatty acid compositions	675:697	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	50	contain	had	630:632	arg2	contents					662:669	substantially different oil contents	634:669	substantially different oil contents	634:669	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	50	contain	had	630:632	arg1	lines					624:628	gene-silenced and bnacipk9 lines	597:628	gene-silenced and bnacipk9 lines	597:628	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	1	51	theme	lipids	191:196	arg1	lipids					191:196	lipids	191:196	lipids	191:196	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	1	51	theme	lipids	191:196	arg1	amounts					180:186	large amounts	174:186	large amounts of lipids	174:196	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	3	52	theme	seed	508:511	arg1	metabolism					517:526	seed oil metabolism	508:526	seed oil metabolism of rapeseeds	508:539	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	4	53	theme	wild	575:578	arg1	type					580:583	wild type	575:583	wild type (WT) lines	575:594	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	53	theme	wild	575:578	arg1	WT					586:587	WT	586:587	WT	586:587	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	3	54	theme	BnaCIPK9	486:493	arg1	functions					495:503	BnaCIPK9 functions	486:503	BnaCIPK9 functions in seed oil metabolism of rapeseeds	486:539	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	6	55	theme	aperture	1379:1386	arg1	assays					1399:1404	stomatal aperture regulation assays	1370:1404	stomatal aperture regulation assays	1370:1404	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	1	56	theme	human	202:206	arg1	requirements					208:219	human requirements	202:219	human requirements	202:219	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	2	57	theme	seed	309:312	arg1	content					318:324	seed oil content	309:324	seed oil content	309:324	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	4	58	theme	gene-silenced	771:783	arg1	lines					785:789	gene-silenced lines	771:789	gene-silenced lines	771:789	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	2	59	theme	protein	259:265	arg1	kinase					267:272	Calcineurin B-like (CBL)-interacting protein kinase 9	222:274	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9)	222:282	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	2	59	theme	protein	259:265	arg1	CIPK9					277:281	CIPK9	277:281	CIPK9	277:281	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	3	60	from	functions	495:503	arg1	metabolism					517:526	seed oil metabolism	508:526	seed oil metabolism of rapeseeds	508:539	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	1	61	theme	oil	145:147	arg1	worldwide					154:162	an important oil crop worldwide	132:162	an important oil crop worldwide	132:162	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	1	61	theme	oil	145:147	arg1	Rapeseed					105:112	Rapeseed	105:112	Rapeseed (Brassica napus)	105:129	Rapeseed (Brassica napus), an important oil crop worldwide, provides large amounts of lipids for human requirements.
36494098	7	62	theme	oil	1663:1665	arg1	accumulation					1667:1678	seed oil accumulation	1658:1678	seed oil accumulation	1658:1678	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	8	63	theme	ABA	1792:1794	arg1	signaling					1804:1812	ABA hormone signaling	1792:1812	ABA hormone signaling	1792:1812	Altogether, our results strongly suggest a role of CIPK9 in mediating the interaction between glucose flux and ABA hormone signaling to regulate seed oil metabolism in rapeseed.
36494098	6	64	theme	genes	1435:1439	arg1	levels					1425:1430	the expression levels	1410:1430	the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds	1410:1500	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	6	64	theme	genes	1435:1439	arg1	assays					1399:1404	stomatal aperture regulation assays	1370:1404	stomatal aperture regulation assays	1370:1404	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	3	65	dep	interference	387:398	arg1	biotechnology					400:412	biotechnology	400:412	biotechnology	400:412	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	7	66	theme	Arabidopsis	1528:1538	arg1	thaliana					1540:1547	Arabidopsis (Arabidopsis thaliana)	1515:1548	Arabidopsis (Arabidopsis thaliana)	1515:1548	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	7	67	theme	Arabidopsis	1515:1525	arg1	thaliana					1540:1547	Arabidopsis (Arabidopsis thaliana)	1515:1548	Arabidopsis (Arabidopsis thaliana)	1515:1548	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	8	68	theme	oil	1831:1833	arg1	metabolism					1835:1844	seed oil metabolism	1826:1844	seed oil metabolism	1826:1844	Altogether, our results strongly suggest a role of CIPK9 in mediating the interaction between glucose flux and ABA hormone signaling to regulate seed oil metabolism in rapeseed.
36494098	7	69	theme	signaling	1645:1653	arg1	effects					1618:1624	the effects	1614:1624	the effects of ABA and glucose signaling on seed oil accumulation	1614:1678	Notably, in Arabidopsis (Arabidopsis thaliana), exogenous ABA and glucose imposed on developing seeds revealed the effects of ABA and glucose signaling on seed oil accumulation.
36494098	4	70	theme	acid	681:684	arg1	compositions					686:697	fatty acid compositions	675:697	fatty acid compositions	675:697	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	2	71	theme	Calcineurin	222:232	arg1	kinase					267:272	Calcineurin B-like (CBL)-interacting protein kinase 9	222:274	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9)	222:282	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	2	71	theme	Calcineurin	222:232	arg1	CIPK9					277:281	CIPK9	277:281	CIPK9	277:281	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	6	72	theme	regulating	1469:1478	arg1	pathways					1480:1487	ABA regulating pathways	1465:1487	ABA regulating pathways	1465:1487	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	4	73	theme	decreased	879:887	arg1	levels					889:894	decreased levels	879:894	decreased levels of polyunsaturated fatty acids	879:925	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	0	74	theme	oil	77:79	arg1	metabolism					81:90	seed oil metabolism	72:90	seed oil metabolism	72:90	Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed.
36494098	6	75	from	pathways	1480:1487	arg1	rapeseeds					1492:1500	rapeseeds	1492:1500	rapeseeds	1492:1500	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	4	76	theme	oil	658:660	arg1	contents					662:669	substantially different oil contents	634:669	substantially different oil contents	634:669	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	77	theme	monounsaturated	847:861	arg1	acids					869:873	monounsaturated fatty acids	847:873	monounsaturated fatty acids	847:873	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	78	theme	bnacipk9	1020:1027	arg1	lines					1029:1033	the bnacipk9 lines	1016:1033	the bnacipk9 lines	1016:1033	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	6	79	from	glucose	1453:1459	arg1	rapeseeds					1492:1500	rapeseeds	1492:1500	rapeseeds	1492:1500	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	4	80	theme	acids	869:873	arg1	levels					889:894	decreased levels	879:894	decreased levels of polyunsaturated fatty acids	879:925	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	80	theme	acids	869:873	arg1	levels					837:842	increased levels	827:842	increased levels of monounsaturated fatty acids	827:873	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	81	dep	lines	1010:1014	arg1	showed					1035:1040	showed	1035:1040	showed significant differences	1035:1064	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	5	82	theme	late	1246:1249	arg1	seeds					1274:1278	late maturation development seeds	1246:1278	late maturation development seeds	1246:1278	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	5	83	theme	hexose-to-sucrose	1203:1219	arg1	ratio					1221:1225	a higher hexose-to-sucrose ratio	1194:1225	a higher hexose-to-sucrose ratio	1194:1225	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	0	84	theme	glucose	24:30	arg1	signaling					50:58	glucose and abscisic acid signaling	24:58	glucose and abscisic acid signaling	24:58	Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed.
36494098	8	85	theme	CIPK9	1732:1736	arg1	role					1724:1727	a role	1722:1727	a role of CIPK9 in mediating the interaction between glucose flux and ABA hormone signaling to regulate seed oil metabolism in rapeseed	1722:1856	Altogether, our results strongly suggest a role of CIPK9 in mediating the interaction between glucose flux and ABA hormone signaling to regulate seed oil metabolism in rapeseed.
36494098	4	86	theme	oil	705:707	arg1	content					709:715	seed oil content	700:715	seed oil content	700:715	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	3	87	theme	mutant	435:440	arg1	bnacipk9					442:449	loss-of-function mutant bnacipk9	418:449	loss-of-function mutant bnacipk9	418:449	Here, we generated gene-silenced lines through RNA interference biotechnology and loss-of-function mutant bnacipk9 using CRISPR/Cas9 to further study BnaCIPK9 functions in seed oil metabolism of rapeseeds.
36494098	0	88	theme	abscisic	36:43	arg1	acid					45:48	abscisic acid	36:48	abscisic acid	36:48	Kinase CIPK9 integrates glucose and abscisic acid signaling to regulate seed oil metabolism in rapeseed.
36494098	5	89	theme	acid	1117:1120	arg1	levels					1128:1133	indoleacetic acid and abscisic acid (ABA) levels	1086:1133	indoleacetic acid and abscisic acid (ABA) levels	1086:1133	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	4	90	theme	fatty	915:919	arg1	acids					921:925	polyunsaturated fatty acids	899:925	polyunsaturated fatty acids	899:925	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	91	theme	1	743:743	arg1	%					744:744	%	744:744	%	744:744	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	92	theme	-6	745:746	arg1	%					744:744	%	744:744	%	744:744	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	93	theme	indoleacetic	1086:1097	arg1	acid					1099:1102	indoleacetic acid	1086:1102	indoleacetic acid	1086:1102	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	4	94	theme	type	580:583	arg1	lines					590:594	wild type (WT) lines	575:594	wild type (WT) lines	575:594	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	4	95	theme	%	734:734	arg1	%					737:737	3%-5%	733:737	3%-5%	733:737	We discovered that compared with wild type (WT) lines, gene-silenced and bnacipk9 lines had substantially different oil contents and fatty acid compositions: seed oil content was improved by 3%-5% and 1%-6% in bnacipk9 lines and gene-silenced lines, respectively; both lines were with increased levels of monounsaturated fatty acids and decreased levels of polyunsaturated fatty acids.
36494098	5	96	theme	development	1262:1272	arg1	seeds					1274:1278	late maturation development seeds	1246:1278	late maturation development seeds	1246:1278	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
36494098	6	97	theme	regulation	1388:1397	arg1	assays					1399:1404	stomatal aperture regulation assays	1370:1404	stomatal aperture regulation assays	1370:1404	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	2	98	theme	oil	314:316	arg1	content					318:324	seed oil content	309:324	seed oil content	309:324	Calcineurin B-like (CBL)-interacting protein kinase 9 (CIPK9) was reported to regulate seed oil content in plant.
36494098	6	99	theme	stomatal	1370:1377	arg1	assays					1399:1404	stomatal aperture regulation assays	1370:1404	stomatal aperture regulation assays	1370:1404	Furthermore, the bnacipk9 was less sensitive to glucose and ABA than the WT according to stomatal aperture regulation assays and the expression levels of genes involved in glucose and ABA regulating pathways in rapeseeds.
36494098	5	100	theme	glucose	1148:1154	arg1	contents					1168:1175	glucose and sucrose contents	1148:1175	glucose and sucrose contents	1148:1175	Additionally, hormone and glucose content analyses revealed that compared with WT lines the bnacipk9 lines showed significant differences: in bnacipk9 seeds, indoleacetic acid and abscisic acid (ABA) levels were higher; glucose and sucrose contents were higher with a higher hexose-to-sucrose ratio in bnacipk9 mid to late maturation development seeds.
35705557	0	0	theme	TNF	85:87	arg1	balance					47:53	the balance	43:53	the balance of soluble and membrane-bound TNF	43:87	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	7	1	from	diversity	1107:1115	arg1	members					1157:1163	critical microbial members	1138:1163	critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity	1138:1267	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	7	2	theme	TACE	1255:1258	arg1	activity					1260:1267	TACE activity	1255:1267	TACE activity	1255:1267	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	6	3	theme	microbial	892:900	arg1	composition					902:912	microbial composition	892:912	microbial composition previously observed during acute inflammation	892:958	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	6	4	theme	MCJ-deficient	1012:1024	arg1	mice					1026:1029	MCJ-deficient mice	1012:1029	MCJ-deficient mice	1012:1029	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	0	5	theme	membrane-bound	70:83	arg1	TNF					85:87	soluble and membrane-bound TNF	58:87	soluble and membrane-bound TNF	58:87	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	6	6	from	mice	1026:1029	arg1	model					987:991	the chronic model	975:991	the chronic model of inflammation in MCJ-deficient mice	975:1029	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	2	7	theme	metabolic	340:348	arg1	status					350:355	the metabolic status	336:355	the metabolic status of macrophages	336:370	MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria.
35705557	1	8	theme	genomic	244:250	arg1	factors					281:287	environmental, genomic, microbial and immunological factors	229:287	environmental, genomic, microbial and immunological factors	229:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	0	9	theme	chronic	96:102	arg1	colitis					117:123	chronic experimental colitis	96:123	chronic experimental colitis	96:123	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	3	10	theme	MCJ	515:517	arg1	deficiency					519:528	MCJ deficiency	515:528	MCJ deficiency	515:528	Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency.
35705557	3	11	dep	showed	474:479	arg1	increased					481:489	increased	481:489	showed increased disease severity due to MCJ deficiency	474:528	Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency.
35705557	4	12	theme	tumor	596:600	arg1	factor					611:616	tumor necrosis factor α	596:618	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	4	13	theme	enzyme	631:636	arg1	activity					645:652	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	8	14	theme	tissue	1350:1355	arg1	adaptation					1326:1335	the dynamic metabolic adaptation	1304:1335	the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function	1304:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	1	15	theme	microbial	253:261	arg1	factors					281:287	environmental, genomic, microbial and immunological factors	229:287	environmental, genomic, microbial and immunological factors	229:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	8	16	theme	dynamic	1308:1314	arg1	adaptation					1326:1335	the dynamic metabolic adaptation	1304:1335	the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function	1304:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	6	17	from	inflammation	996:1007	arg1	mice					1026:1029	MCJ-deficient mice	1012:1029	MCJ-deficient mice	1012:1029	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	3	18	theme	colitis	466:472	arg1	model					445:449	an acute murine model	429:449	an acute murine model of DSS-induced colitis	429:472	Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency.
35705557	3	19	theme	acute	432:436	arg1	model					445:449	an acute murine model	429:449	an acute murine model of DSS-induced colitis	429:472	Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency.
35705557	0	20	theme	experimental	104:115	arg1	colitis					117:123	chronic experimental colitis	96:123	chronic experimental colitis	96:123	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	7	21	theme	microbial	1147:1155	arg1	members					1157:1163	critical microbial members	1138:1163	critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity	1138:1267	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	8	22	theme	inflammatory	1368:1379	arg1	changes					1381:1387	chronic inflammatory changes	1360:1387	chronic inflammatory changes mediated by the control of mitochondrial function	1360:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	1	23	theme	complex	164:170	arg1	disease					210:216	a complex, chronic, relapsing and heterogeneous disease	162:216	a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors	162:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	23	theme	complex	164:170	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease (IBD)	126:157	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	0	24	theme	complex	14:20	arg1	dysfunction					24:34	Mitochondrial complex I dysfunction	0:34	Mitochondrial complex I dysfunction	0:34	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	6	25	theme	general	874:880	arg1	absent					965:970	absent	965:970	absent	965:970	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	6	25	theme	general	874:880	arg1	shifts					882:887	the general shifts	870:887	the general shifts in microbial composition previously observed during acute inflammation	870:958	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	6	26	from	model	987:991	arg1	absent					965:970	absent	965:970	absent	965:970	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	6	26	from	model	987:991	arg1	shifts					882:887	the general shifts	870:887	the general shifts in microbial composition previously observed during acute inflammation	870:958	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	6	26	from	model	987:991	arg1	mice					1026:1029	MCJ-deficient mice	1012:1029	MCJ-deficient mice	1012:1029	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	7	27	theme	mitochondrial	1057:1069	arg1	protein					1071:1077	the mitochondrial protein	1053:1077	the mitochondrial protein	1053:1077	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	2	28	theme	mitochondrial	299:311	arg1	protein					313:319	a mitochondrial protein	297:319	a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria	297:414	MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria.
35705557	2	28	theme	mitochondrial	299:311	arg1	MCJ					290:292	MCJ	290:292	MCJ	290:292	MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria.
35705557	0	29	theme	Mitochondrial	0:12	arg1	dysfunction					24:34	Mitochondrial complex I dysfunction	0:34	Mitochondrial complex I dysfunction	0:34	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	7	30	theme	alpha	1101:1105	arg1	diversity					1107:1115	increased alpha diversity	1091:1115	increased alpha diversity	1091:1115	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	7	31	from	reduction	1125:1133	arg1	members					1157:1163	critical microbial members	1138:1163	critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity	1138:1267	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	7	32	theme	increased	1091:1099	arg1	diversity					1107:1115	increased alpha diversity	1091:1115	increased alpha diversity	1091:1115	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	1	33	theme	chronic	173:179	arg1	disease					210:216	a complex, chronic, relapsing and heterogeneous disease	162:216	a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors	162:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	33	theme	chronic	173:179	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease (IBD)	126:157	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	5	34	theme	soluble	818:824	arg1	form					826:829	the soluble form	814:829	the soluble form	814:829	This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype.
35705557	1	35	theme	immunological	267:279	arg1	factors					281:287	environmental, genomic, microbial and immunological factors	229:287	environmental, genomic, microbial and immunological factors	229:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	4	36	theme	augmented	586:594	arg1	activity					645:652	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	8	37	theme	function	1430:1437	arg1	control					1405:1411	the control	1401:1411	the control of mitochondrial function	1401:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	8	38	theme	colon	1344:1348	arg1	tissue					1350:1355	the colon tissue	1340:1355	the colon tissue	1340:1355	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	3	39	theme	DSS-induced	454:464	arg1	colitis					466:472	DSS-induced colitis	454:472	DSS-induced colitis	454:472	Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency.
35705557	0	40	theme	I	22:22	arg1	dysfunction					24:34	Mitochondrial complex I dysfunction	0:34	Mitochondrial complex I dysfunction	0:34	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	4	41	theme	MCJ-deficient	562:574	arg1	mice					576:579	MCJ-deficient mice	562:579	MCJ-deficient mice	562:579	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	5	42	theme	adaptative	699:708	arg1	change					710:715	This adaptative change	694:715	This adaptative change	694:715	This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype.
35705557	4	43	theme	inflammation	680:691	arg1	context					661:667	the context	657:667	the context of chronic inflammation	657:691	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	1	44	theme	relapsing	182:190	arg1	disease					210:216	a complex, chronic, relapsing and heterogeneous disease	162:216	a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors	162:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	44	theme	relapsing	182:190	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease (IBD)	126:157	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	5	45	dep	soluble	752:758	arg1	TNF					778:780	TNF	778:780	TNF	778:780	This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype.
35705557	8	46	theme	mitochondrial	1416:1428	arg1	function					1430:1437	mitochondrial function	1416:1437	mitochondrial function	1416:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	8	47	theme	adaptation	1326:1335	arg1	evidence					1292:1299	evidence	1292:1299	evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function	1292:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	5	48	theme	milder	837:842	arg1	phenotype					844:852	a milder phenotype	835:852	a milder phenotype	835:852	This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype.
35705557	4	49	theme	chronic	672:678	arg1	inflammation					680:691	chronic inflammation	672:691	chronic inflammation	672:691	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	7	50	theme	protein	1071:1077	arg1	lack					1045:1048	the lack	1041:1048	the lack of the mitochondrial protein	1041:1077	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	5	51	theme	phenotype	844:852	arg1	association					799:809	the association	795:809	the association of the soluble form and a milder phenotype	795:852	This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype.
35705557	1	52	theme	heterogeneous	196:208	arg1	disease					210:216	a complex, chronic, relapsing and heterogeneous disease	162:216	a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors	162:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	52	theme	heterogeneous	196:208	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease (IBD)	126:157	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	7	53	theme	critical	1138:1145	arg1	members					1157:1163	critical microbial members	1138:1163	critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity	1138:1267	However, the lack of the mitochondrial protein resulted in increased alpha diversity and the reduction in critical microbial members associated with inflammation, such as Ruminococcus gnavus, which could be associated with TACE activity.
35705557	6	54	from	shifts	882:887	arg1	composition					902:912	microbial composition	892:912	microbial composition previously observed during acute inflammation	892:958	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	8	55	theme	chronic	1360:1366	arg1	changes					1381:1387	chronic inflammatory changes	1360:1387	chronic inflammatory changes mediated by the control of mitochondrial function	1360:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	6	56	theme	chronic	979:985	arg1	model					987:991	the chronic model	975:991	the chronic model of inflammation in MCJ-deficient mice	975:1029	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	3	57	theme	disease	491:497	arg1	severity					499:506	disease severity	491:506	disease severity	491:506	Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency.
35705557	4	58	contain	have	581:584	arg1	mice					576:579	MCJ-deficient mice	562:579	MCJ-deficient mice	562:579	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	4	58	contain	have	581:584	arg2	activity					645:652	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	1	59	theme	Inflammatory	126:137	arg1	IBD					154:156	IBD	154:156	IBD	154:156	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	59	theme	Inflammatory	126:137	arg1	disease					210:216	a complex, chronic, relapsing and heterogeneous disease	162:216	a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors	162:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	59	theme	Inflammatory	126:137	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease (IBD)	126:157	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	6	60	theme	acute	941:945	arg1	inflammation					947:958	acute inflammation	941:958	acute inflammation	941:958	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	6	61	from	absent	965:970	arg1	model					987:991	the chronic model	975:991	the chronic model of inflammation in MCJ-deficient mice	975:1029	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	1	62	theme	bowel	139:143	arg1	IBD					154:156	IBD	154:156	IBD	154:156	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	62	theme	bowel	139:143	arg1	disease					210:216	a complex, chronic, relapsing and heterogeneous disease	162:216	a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors	162:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	1	62	theme	bowel	139:143	arg1	disease					145:151	Inflammatory bowel disease	126:151	Inflammatory bowel disease (IBD)	126:157	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
35705557	4	63	theme	factor	611:616	arg1	TACE					639:642	TACE	639:642	TACE	639:642	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	4	63	theme	factor	611:616	arg1	enzyme					631:636	tumor necrosis factor α converting enzyme	596:636	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	6	64	theme	inflammation	996:1007	arg1	model					987:991	the chronic model	975:991	the chronic model of inflammation in MCJ-deficient mice	975:1029	Interestingly, the general shifts in microbial composition previously observed during acute inflammation were absent in the chronic model of inflammation in MCJ-deficient mice.
35705557	8	65	theme	metabolic	1316:1324	arg1	adaptation					1326:1335	the dynamic metabolic adaptation	1304:1335	the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function	1304:1437	These results provide evidence of the dynamic metabolic adaptation of the colon tissue to chronic inflammatory changes mediated by the control of mitochondrial function.
35705557	4	66	theme	necrosis	602:609	arg1	factor					611:616	tumor necrosis factor α	596:618	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	2	67	theme	translocated	394:405	arg1	bacteria					407:414	translocated bacteria	394:414	translocated bacteria	394:414	MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria.
35705557	2	68	theme	macrophages	360:370	arg1	response					382:389	their response	376:389	their response to translocated bacteria	376:414	MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria.
35705557	2	68	theme	macrophages	360:370	arg1	status					350:355	the metabolic status	336:355	the metabolic status of macrophages	336:370	MCJ is a mitochondrial protein that regulates the metabolic status of macrophages and their response to translocated bacteria.
35705557	3	69	theme	murine	438:443	arg1	model					445:449	an acute murine model	429:449	an acute murine model of DSS-induced colitis	429:472	Previously, an acute murine model of DSS-induced colitis showed increased disease severity due to MCJ deficiency.
35705557	4	70	theme	converting	620:629	arg1	TACE					639:642	TACE	639:642	TACE	639:642	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	4	70	theme	converting	620:629	arg1	enzyme					631:636	tumor necrosis factor α converting enzyme	596:636	augmented tumor necrosis factor α converting enzyme (TACE) activity	586:652	Unexpectedly, we now show that MCJ-deficient mice have augmented tumor necrosis factor α converting enzyme (TACE) activity in the context of chronic inflammation.
35705557	0	71	theme	soluble	58:64	arg1	TNF					85:87	soluble and membrane-bound TNF	58:87	soluble and membrane-bound TNF	58:87	Mitochondrial complex I dysfunction alters the balance of soluble and membrane-bound TNF during chronic experimental colitis.
35705557	5	72	theme	form	826:829	arg1	association					799:809	the association	795:809	the association of the soluble form and a milder phenotype	795:852	This adaptative change likely affects the balance between soluble and transmembrane TNF and supports the association of the soluble form and a milder phenotype.
35705557	1	73	theme	environmental	229:241	arg1	factors					281:287	environmental, genomic, microbial and immunological factors	229:287	environmental, genomic, microbial and immunological factors	229:287	Inflammatory bowel disease (IBD) is a complex, chronic, relapsing and heterogeneous disease induced by environmental, genomic, microbial and immunological factors.
37379323	5	0	theme	B	757:757	arg1	cells					759:763	ST6Gal1-deficient B cells	739:763	ST6Gal1-deficient B cells	739:763	We previously reported that mice with ST6Gal1-deficient B cells have unaltered IgG sialylation.
37379323	4	1	theme	IgG	664:666	arg1	composition					675:685	IgG glycan composition	664:685	IgG glycan composition	664:685	Despite the immunological significance of these carbohydrates, little is known about how IgG glycan composition is regulated.
37379323	7	2	theme	platelet	974:981	arg1	granules					983:990	platelet granules	974:990	platelet granules	974:990	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	3	3	theme	cellular	464:471	arg1	cytotoxicity					473:484	increased antibody-dependent cellular cytotoxicity	435:484	increased antibody-dependent cellular cytotoxicity	435:484	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	8	4	from	ST6Gal1	1174:1180	arg1	platelets					1204:1212	platelets	1204:1212	platelets	1204:1212	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	8	4	from	ST6Gal1	1174:1180	arg1	megakaryocytes					1185:1198	megakaryocytes	1185:1198	megakaryocytes	1185:1198	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	7	5	theme	cell-extrinsic	1051:1064	arg1	site					1066:1069	a B cell-extrinsic site	1047:1069	a B cell-extrinsic site for IgG sialylation	1047:1089	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	7	5	theme	cell-extrinsic	1051:1064	arg1	granules					1023:1030	platelet granules	1014:1030	platelet granules	1014:1030	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	8	6	used	used	1123:1126	arg2	we					1120:1121	we	1120:1121	we	1120:1121	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	1	7	theme	reciprocal	151:160	arg1	protection					162:171	reciprocal protection	151:171	reciprocal protection from both pathogens and autoimmunity	151:208	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	11	8	contain	have	1603:1606	arg1	platelets					1593:1601	platelets	1593:1601	platelets	1593:1601	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	11	8	contain	have	1603:1606	arg2	role					1622:1625	a substantial role	1608:1625	a substantial role	1608:1625	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	11	8	contain	have	1603:1606	arg1	cells					1570:1574	B cells	1568:1574	B cells	1568:1574	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	11	8	contain	have	1603:1606	arg1	plasma					1581:1586	the plasma	1577:1586	the plasma	1577:1586	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	3	9	link	α2,6-linked	495:505	arg1	sialylation					507:517	α2,6-linked sialylation	495:517	α2,6-linked sialylation by the enzyme ST6Gal1	495:539	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	2	10	gly	N-glycosylation	355:369	arg2	N297					327:330	N297	327:330	N297	327:330	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	2	10	gly	N-glycosylation	355:369	arg2	N-glycosylation					355:369	N-glycosylation	355:369	N-glycosylation	355:369	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	2	10	gly	N-glycosylation	355:369	arg2	site					347:350	the conserved site	333:350	the conserved site of N-glycosylation within the Fc domain	333:390	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	10	11	from	change	1456:1461	arg1	sialylation					1470:1480	IgG sialylation	1466:1480	IgG sialylation	1466:1480	We also found that despite targeted ablation of ST6Gal1, no change in IgG sialylation was apparent.
37379323	6	12	theme	IgG	893:895	arg1	sialylation					897:907	impact overall IgG sialylation	878:907	impact overall IgG sialylation	878:907	Likewise, ST6Gal1 released into the plasma by hepatocytes does not significantly impact overall IgG sialylation.
37379323	1	13	theme	IgG	64:66	arg1	class					77:81	The IgG antibody class	60:81	The IgG antibody class	60:81	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	8	14	theme	albumin-Cre	1246:1256	arg1	mouse					1258:1262	an albumin-Cre mouse to also remove it from hepatocytes and the plasma	1243:1312	an albumin-Cre mouse to also remove it from hepatocytes and the plasma	1243:1312	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	4	15	theme	glycan	668:673	arg1	composition					675:685	IgG glycan composition	664:685	IgG glycan composition	664:685	Despite the immunological significance of these carbohydrates, little is known about how IgG glycan composition is regulated.
37379323	3	16	theme	increased	435:443	arg1	cytotoxicity					473:484	increased antibody-dependent cellular cytotoxicity	435:484	increased antibody-dependent cellular cytotoxicity	435:484	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	7	17	theme	IgG	1075:1077	arg1	sialylation					1079:1089	IgG sialylation	1075:1089	IgG sialylation	1075:1089	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	1	18	theme	antibody	68:75	arg1	class					77:81	The IgG antibody class	60:81	The IgG antibody class	60:81	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	9	19	theme	pathological	1372:1383	arg1	phenotype					1385:1393	no overt pathological phenotype	1363:1393	no overt pathological phenotype	1363:1393	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	8	20	theme	factor	1139:1144	arg1	mouse					1158:1162	a platelet factor 4 (Pf4)-Cre mouse	1128:1162	a platelet factor 4 (Pf4)-Cre mouse	1128:1162	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	6	21	theme	impact	878:883	arg1	sialylation					897:907	impact overall IgG sialylation	878:907	impact overall IgG sialylation	878:907	Likewise, ST6Gal1 released into the plasma by hepatocytes does not significantly impact overall IgG sialylation.
37379323	10	22	theme	IgG	1466:1468	arg1	sialylation					1470:1480	IgG sialylation	1466:1480	IgG sialylation	1466:1480	We also found that despite targeted ablation of ST6Gal1, no change in IgG sialylation was apparent.
37379323	6	23	theme	overall	885:891	arg1	sialylation					897:907	impact overall IgG sialylation	878:907	impact overall IgG sialylation	878:907	Likewise, ST6Gal1 released into the plasma by hepatocytes does not significantly impact overall IgG sialylation.
37379323	2	24	theme	conserved	337:345	arg1	site					347:350	the conserved site	333:350	the conserved site of N-glycosylation within the Fc domain	333:390	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	2	24	theme	conserved	337:345	arg1	N297					327:330	N297	327:330	N297	327:330	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	2	24	theme	conserved	337:345	arg1	N-glycosylation					355:369	N-glycosylation	355:369	N-glycosylation	355:369	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	0	25	theme	Platelet-localized	0:17	arg1	ST6Gal1					19:25	Platelet-localized ST6Gal1	0:25	Platelet-localized ST6Gal1	0:25	Platelet-localized ST6Gal1 does not impact IgG sialylation.
37379323	5	26	contain	have	765:768	arg1	mice					729:732	mice	729:732	mice with ST6Gal1-deficient B cells	729:763	We previously reported that mice with ST6Gal1-deficient B cells have unaltered IgG sialylation.
37379323	5	26	contain	have	765:768	arg2	sialylation					784:794	unaltered IgG sialylation	770:794	unaltered IgG sialylation	770:794	We previously reported that mice with ST6Gal1-deficient B cells have unaltered IgG sialylation.
37379323	9	27	contain	had	1359:1361	arg1	viable					1348:1353	viable	1348:1353	viable	1348:1353	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	9	27	contain	had	1359:1361	arg1	strains					1335:1341	The resulting mouse strains	1315:1341	The resulting mouse strains	1315:1341	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	9	27	contain	had	1359:1361	arg2	phenotype					1385:1393	no overt pathological phenotype	1363:1393	no overt pathological phenotype	1363:1393	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	8	28	theme	-Cre	1153:1156	arg1	mouse					1158:1162	a platelet factor 4 (Pf4)-Cre mouse	1128:1162	a platelet factor 4 (Pf4)-Cre mouse	1128:1162	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	7	29	theme	B	1049:1049	arg1	site					1066:1069	a B cell-extrinsic site	1047:1069	a B cell-extrinsic site for IgG sialylation	1047:1089	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	7	29	theme	B	1049:1049	arg1	granules					1023:1030	platelet granules	1014:1030	platelet granules	1014:1030	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	2	30	from	N297	327:330	arg1	composition					312:322	the glycan composition	301:322	the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain	301:390	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	1	31	theme	important	92:100	arg1	basis					102:106	an important basis	89:106	an important basis of the humoral immune response	89:137	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	2	32	theme	Fc	382:383	arg1	domain					385:390	the Fc domain	378:390	the Fc domain	378:390	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	3	33	theme	α2,6-linked	495:505	arg1	sialylation					507:517	α2,6-linked sialylation	495:517	α2,6-linked sialylation by the enzyme ST6Gal1	495:539	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	2	34	theme	heavy	277:281	arg1	chain					283:287	the heavy chain	273:287	the heavy chain	273:287	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	9	35	theme	overt	1366:1370	arg1	phenotype					1385:1393	no overt pathological phenotype	1363:1393	no overt pathological phenotype	1363:1393	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	3	36	theme	core	414:417	arg1	fucose					419:424	core fucose	414:424	core fucose	414:424	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	0	37	theme	IgG	43:45	arg1	sialylation					47:57	impact IgG sialylation	36:57	impact IgG sialylation	36:57	Platelet-localized ST6Gal1 does not impact IgG sialylation.
37379323	11	38	theme	substantial	1610:1620	arg1	role					1622:1625	a substantial role	1608:1625	a substantial role	1608:1625	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	0	39	theme	impact	36:41	arg1	sialylation					47:57	impact IgG sialylation	36:57	impact IgG sialylation	36:57	Platelet-localized ST6Gal1 does not impact IgG sialylation.
37379323	5	40	theme	unaltered	770:778	arg1	sialylation					784:794	unaltered IgG sialylation	770:794	unaltered IgG sialylation	770:794	We previously reported that mice with ST6Gal1-deficient B cells have unaltered IgG sialylation.
37379323	5	41	with	mice	729:732	arg1	cells					759:763	ST6Gal1-deficient B cells	739:763	ST6Gal1-deficient B cells	739:763	We previously reported that mice with ST6Gal1-deficient B cells have unaltered IgG sialylation.
37379323	2	42	theme	N-glycosylation	355:369	arg1	site					347:350	the conserved site	333:350	the conserved site of N-glycosylation within the Fc domain	333:390	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	2	42	theme	N-glycosylation	355:369	arg1	N297					327:330	N297	327:330	N297	327:330	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	2	42	theme	N-glycosylation	355:369	arg1	N-glycosylation					355:369	N-glycosylation	355:369	N-glycosylation	355:369	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	5	43	theme	IgG	780:782	arg1	sialylation					784:794	unaltered IgG sialylation	770:794	unaltered IgG sialylation	770:794	We previously reported that mice with ST6Gal1-deficient B cells have unaltered IgG sialylation.
37379323	8	44	theme	platelet	1130:1137	arg1	factor					1139:1144	platelet factor 4	1130:1146	a platelet factor 4 (Pf4)-Cre mouse	1128:1162	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	8	44	theme	platelet	1130:1137	arg1	Pf4					1149:1151	Pf4	1149:1151	Pf4	1149:1151	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	8	45	attach	remove	1272:1277	arg2	mouse					1258:1262	an albumin-Cre mouse to also remove it from hepatocytes and the plasma	1243:1312	an albumin-Cre mouse to also remove it from hepatocytes and the plasma	1243:1312	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	8	45	attach	remove	1272:1277	arg1	hepatocytes					1287:1297	hepatocytes	1287:1297	hepatocytes	1287:1297	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	8	45	attach	remove	1272:1277	arg1	plasma					1307:1312	the plasma	1303:1312	the plasma	1303:1312	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	2	46	theme	glycan	305:310	arg1	composition					312:322	the glycan composition	301:322	the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain	301:390	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	10	47	theme	targeted	1423:1430	arg1	ablation					1432:1439	targeted ablation	1423:1439	targeted ablation of ST6Gal1	1423:1450	We also found that despite targeted ablation of ST6Gal1, no change in IgG sialylation was apparent.
37379323	10	48	theme	ST6Gal1	1444:1450	arg1	ablation					1432:1439	targeted ablation	1423:1439	targeted ablation of ST6Gal1	1423:1450	We also found that despite targeted ablation of ST6Gal1, no change in IgG sialylation was apparent.
37379323	2	49	theme	IgG	211:213	arg1	function					215:222	IgG function	211:222	IgG function	211:222	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	3	50	theme	immune	556:561	arg1	quiescence					563:572	immune quiescence	556:572	immune quiescence	556:572	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	11	51	theme	prior	1514:1518	arg1	findings					1520:1527	our prior findings	1510:1527	our prior findings	1510:1527	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	3	52	theme	antibody-dependent	445:462	arg1	cytotoxicity					473:484	increased antibody-dependent cellular cytotoxicity	435:484	increased antibody-dependent cellular cytotoxicity	435:484	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	9	53	theme	resulting	1319:1327	arg1	viable					1348:1353	viable	1348:1353	viable	1348:1353	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	9	53	theme	resulting	1319:1327	arg1	strains					1335:1341	The resulting mouse strains	1315:1341	The resulting mouse strains	1315:1341	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	7	54	theme	platelet	1014:1021	arg1	site					1066:1069	a B cell-extrinsic site	1047:1069	a B cell-extrinsic site for IgG sialylation	1047:1089	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	7	54	theme	platelet	1014:1021	arg1	granules					1023:1030	platelet granules	1014:1030	platelet granules	1014:1030	Since IgG and ST6Gal1 have independently been shown to exist in platelet granules, it was possible that platelet granules could serve as a B cell-extrinsic site for IgG sialylation.
37379323	1	55	from	autoimmunity	197:208	arg1	protection					162:171	reciprocal protection	151:171	reciprocal protection from both pathogens and autoimmunity	151:208	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	1	56	theme	humoral	115:121	arg1	response					130:137	the humoral immune response	111:137	the humoral immune response	111:137	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	3	57	theme	enzyme	526:531	arg1	ST6Gal1					533:539	the enzyme ST6Gal1	522:539	the enzyme ST6Gal1	522:539	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	9	58	theme	mouse	1329:1333	arg1	viable					1348:1353	viable	1348:1353	viable	1348:1353	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	9	58	theme	mouse	1329:1333	arg1	strains					1335:1341	The resulting mouse strains	1315:1341	The resulting mouse strains	1315:1341	The resulting mouse strains were viable and had no overt pathological phenotype.
37379323	1	59	theme	immune	123:128	arg1	response					130:137	the humoral immune response	111:137	the humoral immune response	111:137	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	11	60	theme	B	1568:1568	arg1	cells					1570:1574	B cells	1568:1574	B cells	1568:1574	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	3	61	theme	fucose	419:424	arg1	lack					406:409	lack	406:409	lack of core fucose	406:424	For example, lack of core fucose promotes increased antibody-dependent cellular cytotoxicity, whereas α2,6-linked sialylation by the enzyme ST6Gal1 helps to drive immune quiescence.
37379323	4	62	theme	carbohydrates	623:635	arg1	significance					601:612	the immunological significance	583:612	the immunological significance of these carbohydrates	583:635	Despite the immunological significance of these carbohydrates, little is known about how IgG glycan composition is regulated.
37379323	2	63	theme	IgG	245:247	arg1	subclass					249:256	the IgG subclass	241:256	the IgG subclass	241:256	IgG function is determined by the IgG subclass, as defined by the heavy chain, as well as the glycan composition at N297, the conserved site of N-glycosylation within the Fc domain.
37379323	1	64	from	pathogens	183:191	arg1	protection					162:171	reciprocal protection	151:171	reciprocal protection from both pathogens and autoimmunity	151:208	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	1	65	theme	response	130:137	arg1	basis					102:106	an important basis	89:106	an important basis of the humoral immune response	89:137	The IgG antibody class forms an important basis of the humoral immune response, conferring reciprocal protection from both pathogens and autoimmunity.
37379323	11	66	theme	homeostatic	1630:1640	arg1	sialylation					1646:1656	homeostatic IgG sialylation	1630:1656	homeostatic IgG sialylation	1630:1656	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	5	67	theme	ST6Gal1-deficient	739:755	arg1	cells					759:763	ST6Gal1-deficient B cells	739:763	ST6Gal1-deficient B cells	739:763	We previously reported that mice with ST6Gal1-deficient B cells have unaltered IgG sialylation.
37379323	8	68	with	combination	1226:1236	arg1	mouse					1258:1262	an albumin-Cre mouse to also remove it from hepatocytes and the plasma	1243:1312	an albumin-Cre mouse to also remove it from hepatocytes and the plasma	1243:1312	To address this hypothesis, we used a platelet factor 4 (Pf4)-Cre mouse to delete ST6Gal1 in megakaryocytes and platelets alone or in combination with an albumin-Cre mouse to also remove it from hepatocytes and the plasma.
37379323	11	69	theme	IgG	1642:1644	arg1	sialylation					1646:1656	homeostatic IgG sialylation	1630:1656	homeostatic IgG sialylation	1630:1656	Together with our prior findings, we can conclude that in mice, neither B cells, the plasma, nor platelets have a substantial role in homeostatic IgG sialylation.
37379323	4	70	theme	immunological	587:599	arg1	significance					601:612	the immunological significance	583:612	the immunological significance of these carbohydrates	583:635	Despite the immunological significance of these carbohydrates, little is known about how IgG glycan composition is regulated.
37019903	7	0	theme	tetrameric	1138:1147	arg1	complex					1166:1172	the tetrameric TpoR-CALR mutant complex	1134:1172	the tetrameric TpoR-CALR mutant complex	1134:1172	Finally, we propose a model of the tetrameric TpoR-CALR mutant complex and identify potentially targetable sites.
37019903	3	1	theme	constitutive	531:542	arg1	activation					544:553	its constitutive activation	527:553	its constitutive activation	527:553	Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation.
37019903	6	2	theme	domain	1041:1046	arg1	patches					1011:1017	acidic patches	1004:1017	acidic patches of TpoR extracellular domain	1004:1046	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	2	3	gly	N-glycosylated	321:334	arg1	proteins					336:343	immature N-glycosylated proteins	312:343	immature N-glycosylated proteins	312:343	In healthy cells, CALR transiently and non-specifically interacts with immature N-glycosylated proteins through its N-terminal domain.
37019903	7	4	theme	TpoR-CALR	1149:1157	arg1	complex					1166:1172	the tetrameric TpoR-CALR mutant complex	1134:1172	the tetrameric TpoR-CALR mutant complex	1134:1172	Finally, we propose a model of the tetrameric TpoR-CALR mutant complex and identify potentially targetable sites.
37019903	4	5	theme	specificity	600:610	arg1	basis					578:582	the basis	574:582	the basis of the acquired specificity of CALR mutants for TpoR	574:635	Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation.
37019903	3	6	theme	CALR	388:391	arg1	mutants					404:410	CALR frameshift mutants	388:410	CALR frameshift mutants	388:410	Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation.
37019903	4	7	theme	mutants	620:626	arg1	specificity					600:610	the acquired specificity	587:610	the acquired specificity of CALR mutants for TpoR	587:635	Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation.
37019903	0	8	theme	complex	97:103	arg1	dimerization					105:116	complex dimerization	97:116	complex dimerization	97:116	Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation.
37019903	5	9	dep	bind	842:845	arg1	accessible					828:837	accessible	828:837	accessible	828:837	Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR.
37019903	4	10	theme	CALR	615:618	arg1	mutants					620:626	CALR mutants	615:626	CALR mutants	615:626	Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation.
37019903	5	11	from	N-glycans	856:864	arg1	TpoR					869:872	TpoR	869:872	TpoR	869:872	Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR.
37019903	6	12	theme	mutant	1086:1091	arg1	dimerization					1060:1071	dimerization	1060:1071	dimerization of both CALR mutant and TpoR	1060:1100	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	6	13	theme	mutant	906:911	arg1	C-terminus					913:922	the basic mutant C-terminus	896:922	the basic mutant C-terminus	896:922	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	6	13	theme	mutant	906:911	arg1	α-helical					937:945	α-helical	937:945	α-helical	937:945	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	0	14	theme	Oncogenic	0:8	arg1	C-terminus					22:31	Oncogenic CALR mutant C-terminus	0:31	Oncogenic CALR mutant C-terminus	0:31	Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation.
37019903	1	15	theme	second	189:194	arg1	cause					196:200	the second cause	185:200	the second cause of myeloproliferative neoplasms (MPN)	185:238	Calreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN).
37019903	0	16	theme	CALR	10:13	arg1	C-terminus					22:31	Oncogenic CALR mutant C-terminus	0:31	Oncogenic CALR mutant C-terminus	0:31	Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation.
37019903	6	17	theme	basic	900:904	arg1	C-terminus					913:922	the basic mutant C-terminus	896:922	the basic mutant C-terminus	896:922	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	6	17	theme	basic	900:904	arg1	α-helical					937:945	α-helical	937:945	α-helical	937:945	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	5	18	theme	CALR	786:789	arg1	domain					802:807	CALR N-terminal domain	786:807	CALR N-terminal domain	786:807	Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR.
37019903	4	19	theme	TpoR	699:702	arg1	dimerization					704:715	TpoR dimerization	699:715	TpoR dimerization	699:715	Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation.
37019903	3	20	theme	rogue	422:426	arg1	cytokines					428:436	rogue cytokines	422:436	rogue cytokines	422:436	Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation.
37019903	6	21	theme	TpoR	1097:1100	arg1	dimerization					1060:1071	dimerization	1060:1071	dimerization of both CALR mutant and TpoR	1060:1100	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	5	22	theme	immature	847:854	arg1	N-glycans					856:864	immature N-glycans	847:864	immature N-glycans on TpoR	847:872	Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR.
37019903	6	23	theme	acidic	1004:1009	arg1	patches					1011:1017	acidic patches	1004:1017	acidic patches of TpoR extracellular domain	1004:1046	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	2	24	theme	N-glycosylated	321:334	arg1	proteins					336:343	immature N-glycosylated proteins	312:343	immature N-glycosylated proteins	312:343	In healthy cells, CALR transiently and non-specifically interacts with immature N-glycosylated proteins through its N-terminal domain.
37019903	1	25	theme	myeloproliferative	205:222	arg1	neoplasms					224:232	myeloproliferative neoplasms	205:232	myeloproliferative neoplasms (MPN)	205:238	Calreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN).
37019903	1	25	theme	myeloproliferative	205:222	arg1	MPN					235:237	MPN	235:237	MPN	235:237	Calreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN).
37019903	0	26	theme	mutant	15:20	arg1	C-terminus					22:31	Oncogenic CALR mutant C-terminus	0:31	Oncogenic CALR mutant C-terminus	0:31	Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation.
37019903	4	27	theme	acquired	591:598	arg1	specificity					600:610	the acquired specificity	587:610	the acquired specificity of CALR mutants for TpoR	587:635	Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation.
37019903	2	28	theme	immature	312:319	arg1	proteins					336:343	immature N-glycosylated proteins	312:343	immature N-glycosylated proteins	312:343	In healthy cells, CALR transiently and non-specifically interacts with immature N-glycosylated proteins through its N-terminal domain.
37019903	1	29	theme	neoplasms	224:232	arg1	cause					196:200	the second cause	185:200	the second cause of myeloproliferative neoplasms (MPN)	185:238	Calreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN).
37019903	0	30	theme	dual	42:45	arg1	binding					47:53	dual binding	42:53	dual binding to the thrombopoietin receptor triggering complex dimerization and activation	42:131	Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation.
37019903	6	31	theme	CALR	1081:1084	arg1	mutant					1086:1091	CALR mutant	1081:1091	CALR mutant	1081:1091	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	2	32	theme	healthy	244:250	arg1	cells					252:256	healthy cells	244:256	healthy cells	244:256	In healthy cells, CALR transiently and non-specifically interacts with immature N-glycosylated proteins through its N-terminal domain.
37019903	3	33	theme	Thrombopoietin	486:499	arg1	Receptor					501:508	Thrombopoietin Receptor	486:508	the Thrombopoietin Receptor (TpoR)	482:515	Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation.
37019903	3	33	theme	Thrombopoietin	486:499	arg1	TpoR					511:514	TpoR	511:514	TpoR	511:514	Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation.
37019903	6	34	theme	extracellular	1027:1039	arg1	domain					1041:1046	TpoR extracellular domain	1022:1046	TpoR extracellular domain	1022:1046	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	5	35	theme	mutant	760:765	arg1	C-terminus					767:776	CALR mutant C-terminus	755:776	CALR mutant C-terminus	755:776	Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR.
37019903	3	36	dep	interacting	465:475	arg1	inducing					518:525	inducing	518:525	inducing its constitutive activation	518:553	Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation.
37019903	7	37	theme	mutant	1159:1164	arg1	complex					1166:1172	the tetrameric TpoR-CALR mutant complex	1134:1172	the tetrameric TpoR-CALR mutant complex	1134:1172	Finally, we propose a model of the tetrameric TpoR-CALR mutant complex and identify potentially targetable sites.
37019903	5	38	theme	CALR	755:758	arg1	C-terminus					767:776	CALR mutant C-terminus	755:776	CALR mutant C-terminus	755:776	Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR.
37019903	7	39	theme	complex	1166:1172	arg1	model					1125:1129	a model	1123:1129	a model of the tetrameric TpoR-CALR mutant complex	1123:1172	Finally, we propose a model of the tetrameric TpoR-CALR mutant complex and identify potentially targetable sites.
37019903	1	40	theme	Calreticulin	134:145	arg1	mutations					165:173	Calreticulin (CALR) frameshift mutations	134:173	Calreticulin (CALR) frameshift mutations	134:173	Calreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN).
37019903	2	41	theme	N-terminal	357:366	arg1	domain					368:373	its N-terminal domain	353:373	its N-terminal domain	353:373	In healthy cells, CALR transiently and non-specifically interacts with immature N-glycosylated proteins through its N-terminal domain.
37019903	6	42	theme	TpoR	1022:1025	arg1	domain					1041:1046	TpoR extracellular domain	1022:1046	TpoR extracellular domain	1022:1046	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	0	43	theme	thrombopoietin	62:75	arg1	receptor					77:84	the thrombopoietin receptor	58:84	the thrombopoietin receptor triggering complex dimerization and activation	58:131	Oncogenic CALR mutant C-terminus mediates dual binding to the thrombopoietin receptor triggering complex dimerization and activation.
37019903	4	44	theme	complex	672:678	arg1	formation					680:688	complex formation	672:688	complex formation	672:688	Here, we identify the basis of the acquired specificity of CALR mutants for TpoR and define the mechanisms by which complex formation triggers TpoR dimerization and activation.
37019903	5	45	theme	N-terminal	791:800	arg1	domain					802:807	CALR N-terminal domain	786:807	CALR N-terminal domain	786:807	Our work reveals that CALR mutant C-terminus unmasks CALR N-terminal domain, rendering it more accessible to bind immature N-glycans on TpoR.
37019903	7	46	theme	targetable	1199:1208	arg1	sites					1210:1214	potentially targetable sites	1187:1214	potentially targetable sites	1187:1214	Finally, we propose a model of the tetrameric TpoR-CALR mutant complex and identify potentially targetable sites.
37019903	1	47	theme	frameshift	154:163	arg1	mutations					165:173	Calreticulin (CALR) frameshift mutations	134:173	Calreticulin (CALR) frameshift mutations	134:173	Calreticulin (CALR) frameshift mutations represent the second cause of myeloproliferative neoplasms (MPN).
37019903	6	48	theme	α-helical	966:974	arg1	segment					976:982	its α-helical segment	962:982	its α-helical segment	962:982	We further find that the basic mutant C-terminus is partially α-helical and define how its α-helical segment concomitantly binds acidic patches of TpoR extracellular domain and induces dimerization of both CALR mutant and TpoR.
37019903	3	49	theme	frameshift	393:402	arg1	mutants					404:410	CALR frameshift mutants	388:410	CALR frameshift mutants	388:410	Conversely, CALR frameshift mutants turn into rogue cytokines by stably and specifically interacting with the Thrombopoietin Receptor (TpoR), inducing its constitutive activation.
36155291	0	0	theme	membrane	103:110	arg1	protein					112:118	donkey milk fat globule membrane protein	79:118	donkey milk fat globule membrane protein	79:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	4	1	gly	N-glycoproteins	692:706	arg1	N-glycoproteins					692:706	DC MFGM N-glycoproteins	684:706	DC MFGM N-glycoproteins	684:706	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	1	2	theme	post-translational	181:198	arg1	N-glycosylation					138:152	N-glycosylation	138:152	N-glycosylation	138:152	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	1	2	theme	post-translational	181:198	arg1	modification					200:211	a prevalent and complex post-translational modification	157:211	a prevalent and complex post-translational modification of milk proteins with significant biological importance	157:267	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	0	3	theme	globule	95:101	arg1	protein					112:118	donkey milk fat globule membrane protein	79:118	donkey milk fat globule membrane protein	79:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	7	4	from	functions	1294:1302	arg1	composition					1201:1211	the composition	1197:1211	the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions	1197:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	1	5	with	modification	200:211	arg1	importance					258:267	significant biological importance	235:267	significant biological importance	235:267	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	7	6	theme	MFGM	1223:1226	arg1	N-glycoproteins					1228:1242	donkey MFGM N-glycoproteins	1216:1242	donkey MFGM N-glycoproteins	1216:1242	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	3	7	theme	intact	410:415	arg1	N-glycopeptides					417:431	1443 intact N-glycopeptides	405:431	1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	405:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	2	8	theme	donkey	314:319	arg1	N-glycoproteins					354:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	6	9	gly	fucosylation	1064:1075	arg1	N-glycans					1017:1025	the site-specific N-glycans	999:1025	the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation	999:1091	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	5	10	gly	glycoproteins	831:843	arg1	glycoproteins					831:843	eight glycoproteins	825:843	eight glycoproteins	825:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	3	11	theme	site-specific	602:614	arg1	glycoproteomics					616:630	label-free site-specific glycoproteomics	591:630	label-free site-specific glycoproteomics	591:630	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	7	12	from	composition	1201:1211	arg1	functions					1294:1302	donkey milk-related biological functions	1263:1302	donkey milk-related biological functions	1263:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	0	13	theme	protein	112:118	arg1	N-glycosylation					60:74	differential site-specific N-glycosylation	33:74	differential site-specific N-glycosylation of donkey milk fat globule membrane protein	33:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	6	14	theme	site-specific	1003:1015	arg1	N-glycans					1017:1025	the site-specific N-glycans	999:1025	the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation	999:1091	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	7	15	theme	roles	1254:1258	arg1	composition					1201:1211	the composition	1197:1211	the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions	1197:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	2	16	theme	membrane	338:345	arg1	N-glycoproteins					354:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	5	17	gly	glycosites	925:934	arg1	glycoproteins					942:954	16 glycoproteins	939:954	16 glycoproteins	939:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	17	gly	glycosites	925:934	arg2	glycosites					925:934	19 glycosites	922:934	19 glycosites of 16 glycoproteins	922:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	17	gly	glycosites	925:934	arg2	glycoproteins					942:954	16 glycoproteins	939:954	16 glycoproteins	939:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	4	18	from	predominant	669:679	arg1	N-glycoproteins					692:706	DC MFGM N-glycoproteins	684:706	DC MFGM N-glycoproteins	684:706	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	3	19	gly	glycoproteins	447:459	arg1	glycoproteins					447:459	336 MFGM glycoproteins	438:459	336 MFGM glycoproteins in donkey colostrum (DC)	438:484	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	5	20	attach	attached	910:917	arg2	N-glycans					900:908	30 site-specific N-glycans	883:908	30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins	883:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	20	attach	attached	910:917	arg1	glycosites					925:934	19 glycosites	922:934	19 glycosites of 16 glycoproteins	922:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	20	attach	attached	910:917	arg1	glycoproteins					942:954	16 glycoproteins	939:954	16 glycoproteins	939:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	2	21	theme	globule	330:336	arg1	N-glycoproteins					354:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	5	22	theme	glycoproteins	942:954	arg1	glycosites					925:934	19 glycosites	922:934	19 glycosites of 16 glycoproteins	922:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	22	theme	glycoproteins	942:954	arg1	glycoproteins					942:954	16 glycoproteins	939:954	16 glycoproteins	939:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	7	23	theme	milk-related	1270:1281	arg1	functions					1294:1302	donkey milk-related biological functions	1263:1302	donkey milk-related biological functions	1263:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	5	24	theme	site-specific	886:898	arg1	N-glycans					900:908	30 site-specific N-glycans	883:908	30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins	883:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	2	25	theme	fat	326:328	arg1	N-glycoproteins					354:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	4	26	from	mannosylation	736:748	arg1	DM					753:754	DM	753:754	DM	753:754	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	1	27	theme	milk	216:219	arg1	proteins					221:228	milk proteins	216:228	milk proteins	216:228	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	3	28	theme	MFGM	442:445	arg1	glycoproteins					447:459	336 MFGM glycoproteins	438:459	336 MFGM glycoproteins in donkey colostrum (DC)	438:484	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	29	from	glycoproteins	447:459	arg1	DC					482:483	DC	482:483	DC	482:483	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	29	from	glycoproteins	447:459	arg1	colostrum					471:479	donkey colostrum	464:479	donkey colostrum (DC)	464:484	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	29	from	glycoproteins	447:459	arg1	N-glycopeptides					417:431	1443 intact N-glycopeptides	405:431	1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	405:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	5	30	gly	glycoproteins	942:954	arg1	glycoproteins					942:954	16 glycoproteins	939:954	16 glycoproteins	939:954	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	0	31	theme	fat	91:93	arg1	protein					112:118	donkey milk fat globule membrane protein	79:118	donkey milk fat globule membrane protein	79:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	1	32	theme	proteins	221:228	arg1	N-glycosylation					138:152	N-glycosylation	138:152	N-glycosylation	138:152	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	1	32	theme	proteins	221:228	arg1	modification					200:211	a prevalent and complex post-translational modification	157:211	a prevalent and complex post-translational modification of milk proteins with significant biological importance	157:267	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	0	33	theme	Glycoproteomics	0:14	arg1	analysis					16:23	Glycoproteomics analysis	0:23	Glycoproteomics analysis	0:23	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	4	34	gly	sialylation	720:730	arg1	DM					753:754	DM	753:754	DM	753:754	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	7	35	from	roles	1254:1258	arg1	functions					1294:1302	donkey milk-related biological functions	1263:1302	donkey milk-related biological functions	1263:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	2	36	theme	N-glycoproteins	354:368	arg1	characterisation					294:309	the systematic characterisation	279:309	the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins	279:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	4	37	from	sialylation	720:730	arg1	DM					753:754	DM	753:754	DM	753:754	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	4	38	gly	mannosylation	736:748	arg1	DM					753:754	DM	753:754	DM	753:754	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	0	39	theme	differential	33:44	arg1	N-glycosylation					60:74	differential site-specific N-glycosylation	33:74	differential site-specific N-glycosylation of donkey milk fat globule membrane protein	33:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	3	40	gly	N-glycopeptides	417:431	arg2	N-glycopeptides					417:431	1443 intact N-glycopeptides	405:431	1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	405:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	40	gly	N-glycopeptides	417:431	arg1	N-glycopeptides					501:515	489 intact N-glycopeptides	490:515	489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	490:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	40	gly	N-glycopeptides	417:431	arg1	glycoproteins					447:459	336 MFGM glycoproteins	438:459	336 MFGM glycoproteins in donkey colostrum (DC)	438:484	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	41	gly	glycoproteins	530:542	arg1	glycoproteins					530:542	86 MFGM glycoproteins	522:542	86 MFGM glycoproteins in donkey mature milk (DM)	522:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	4	42	attach	predominant	669:679	arg2	Mannosylation					633:645	Mannosylation	633:645	Mannosylation	633:645	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	4	42	attach	predominant	669:679	arg1	N-glycoproteins					692:706	DC MFGM N-glycoproteins	684:706	DC MFGM N-glycoproteins	684:706	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	4	42	attach	predominant	669:679	arg2	fucosylation					651:662	fucosylation	651:662	fucosylation	651:662	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	1	43	theme	significant	235:245	arg1	importance					258:267	significant biological importance	235:267	significant biological importance	235:267	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	7	44	from	N-glycoproteins	1228:1242	arg1	functions					1294:1302	donkey milk-related biological functions	1263:1302	donkey milk-related biological functions	1263:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	5	45	theme	site-specific	772:784	arg1	N-glycans					786:794	22 site-specific N-glycans	769:794	22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins	769:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	2	46	theme	MFGM	348:351	arg1	N-glycoproteins					354:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	1	47	theme	biological	247:256	arg1	importance					258:267	significant biological importance	235:267	significant biological importance	235:267	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	3	48	theme	mature	554:559	arg1	milk					561:564	donkey mature milk	547:564	donkey mature milk (DM)	547:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	48	theme	mature	554:559	arg1	DM					567:568	DM	567:568	DM	567:568	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	2	49	theme	systematic	283:292	arg1	characterisation					294:309	the systematic characterisation	279:309	the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins	279:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	4	50	from	N-glycoproteins	692:706	arg1	predominant					669:679	predominant	669:679	predominant	669:679	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	3	51	theme	intact	494:499	arg1	N-glycopeptides					501:515	489 intact N-glycopeptides	490:515	489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	490:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	0	52	theme	site-specific	46:58	arg1	N-glycosylation					60:74	differential site-specific N-glycosylation	33:74	differential site-specific N-glycosylation of donkey milk fat globule membrane protein	33:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	6	53	theme	site	1150:1153	arg1	specificity					1155:1165	significant site specificity	1138:1165	significant site specificity	1138:1165	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	0	54	theme	donkey	79:84	arg1	protein					112:118	donkey milk fat globule membrane protein	79:118	donkey milk fat globule membrane protein	79:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	6	55	theme	simultaneous	1051:1062	arg1	fucosylation					1064:1075	fucosylation	1064:1075	fucosylation	1064:1075	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	7	56	theme	new	1179:1181	arg1	insights					1183:1190	new insights	1179:1190	new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions	1179:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	5	57	theme	glycoproteins	831:843	arg1	glycosites					811:820	14 glycosites	808:820	14 glycosites of eight glycoproteins	808:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	57	theme	glycoproteins	831:843	arg1	glycoproteins					831:843	eight glycoproteins	825:843	eight glycoproteins	825:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	3	58	gly	N-glycopeptides	501:515	arg2	N-glycopeptides					501:515	489 intact N-glycopeptides	490:515	489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	490:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	58	gly	N-glycopeptides	501:515	arg1	glycoproteins					530:542	86 MFGM glycoproteins	522:542	86 MFGM glycoproteins in donkey mature milk (DM)	522:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	59	from	N-glycopeptides	501:515	arg1	DC					482:483	DC	482:483	DC	482:483	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	59	from	N-glycopeptides	501:515	arg1	colostrum					471:479	donkey colostrum	464:479	donkey colostrum (DC)	464:484	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	59	from	N-glycopeptides	501:515	arg1	N-glycopeptides					417:431	1443 intact N-glycopeptides	405:431	1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	405:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	6	60	with	N-glycans	1017:1025	arg1	moieties					1039:1046	Neu5Gc moieties	1032:1046	Neu5Gc moieties	1032:1046	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	6	60	with	N-glycans	1017:1025	arg1	fucosylation					1064:1075	fucosylation	1064:1075	fucosylation	1064:1075	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	3	61	theme	donkey	464:469	arg1	DC					482:483	DC	482:483	DC	482:483	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	61	theme	donkey	464:469	arg1	colostrum					471:479	donkey colostrum	464:479	donkey colostrum (DC)	464:484	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	7	62	theme	donkey	1216:1221	arg1	N-glycoproteins					1228:1242	donkey MFGM N-glycoproteins	1216:1242	donkey MFGM N-glycoproteins	1216:1242	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	5	63	dep	increased	864:872	arg1	whereas					875:881	whereas	875:881	whereas	875:881	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	3	64	theme	donkey	547:552	arg1	milk					561:564	donkey mature milk	547:564	donkey mature milk (DM)	547:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	64	theme	donkey	547:552	arg1	DM					567:568	DM	567:568	DM	567:568	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	7	65	theme	donkey	1263:1268	arg1	functions					1294:1302	donkey milk-related biological functions	1263:1302	donkey milk-related biological functions	1263:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	7	66	theme	biological	1283:1292	arg1	functions					1294:1302	donkey milk-related biological functions	1263:1302	donkey milk-related biological functions	1263:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	2	67	theme	milk	321:324	arg1	N-glycoproteins					354:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	7	68	theme	N-glycoproteins	1228:1242	arg1	composition					1201:1211	the composition	1197:1211	the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions	1197:1302	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	3	69	theme	MFGM	525:528	arg1	glycoproteins					530:542	86 MFGM glycoproteins	522:542	86 MFGM glycoproteins in donkey mature milk (DM)	522:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	70	theme	label-free	591:600	arg1	glycoproteomics					616:630	label-free site-specific glycoproteomics	591:630	label-free site-specific glycoproteomics	591:630	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	0	71	gly	N-glycosylation	60:74	arg1	protein					112:118	donkey milk fat globule membrane protein	79:118	donkey milk fat globule membrane protein	79:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	1	72	theme	prevalent	159:167	arg1	N-glycosylation					138:152	N-glycosylation	138:152	N-glycosylation	138:152	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	1	72	theme	prevalent	159:167	arg1	modification					200:211	a prevalent and complex post-translational modification	157:211	a prevalent and complex post-translational modification of milk proteins with significant biological importance	157:267	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	5	73	attach	attached	796:803	arg1	glycosites					811:820	14 glycosites	808:820	14 glycosites of eight glycoproteins	808:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	73	attach	attached	796:803	arg1	glycoproteins					831:843	eight glycoproteins	825:843	eight glycoproteins	825:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	73	attach	attached	796:803	arg2	N-glycans					786:794	22 site-specific N-glycans	769:794	22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins	769:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	2	74	gly	N-glycoproteins	354:368	arg1	N-glycoproteins					354:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	donkey milk fat globule membrane (MFGM) N-glycoproteins	314:368	However, the systematic characterisation of donkey milk fat globule membrane (MFGM) N-glycoproteins remains largely ill-defined.
36155291	3	75	from	glycoproteins	530:542	arg1	N-glycopeptides					501:515	489 intact N-glycopeptides	490:515	489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM)	490:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	75	from	glycoproteins	530:542	arg1	glycoproteins					447:459	336 MFGM glycoproteins	438:459	336 MFGM glycoproteins in donkey colostrum (DC)	438:484	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	75	from	glycoproteins	530:542	arg1	DM					567:568	DM	567:568	DM	567:568	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	3	75	from	glycoproteins	530:542	arg1	milk					561:564	donkey mature milk	547:564	donkey mature milk (DM)	547:569	Here, 1443 intact N-glycopeptides from 336 MFGM glycoproteins in donkey colostrum (DC) and 489 intact N-glycopeptides from 86 MFGM glycoproteins in donkey mature milk (DM) were identified via label-free site-specific glycoproteomics.
36155291	7	76	gly	N-glycoproteins	1228:1242	arg1	N-glycoproteins					1228:1242	donkey MFGM N-glycoproteins	1216:1242	donkey MFGM N-glycoproteins	1216:1242	We provide new insights into the composition of donkey MFGM N-glycoproteins and their roles in donkey milk-related biological functions.
36155291	4	77	theme	MFGM	687:690	arg1	N-glycoproteins					692:706	DC MFGM N-glycoproteins	684:706	DC MFGM N-glycoproteins	684:706	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36155291	0	78	theme	milk	86:89	arg1	protein					112:118	donkey milk fat globule membrane protein	79:118	donkey milk fat globule membrane protein	79:118	Glycoproteomics analysis reveals differential site-specific N-glycosylation of donkey milk fat globule membrane protein during lactation.
36155291	6	79	theme	Neu5Gc	1032:1037	arg1	moieties					1039:1046	Neu5Gc moieties	1032:1046	Neu5Gc moieties	1032:1046	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	6	80	theme	significant	1138:1148	arg1	specificity					1155:1165	significant site specificity	1138:1165	significant site specificity	1138:1165	Furthermore, the site-specific N-glycans with Neu5Gc moieties or simultaneous fucosylation and sialylation were not significantly increased, exhibiting significant site specificity.
36155291	1	81	theme	complex	173:179	arg1	N-glycosylation					138:152	N-glycosylation	138:152	N-glycosylation	138:152	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	1	81	theme	complex	173:179	arg1	modification					200:211	a prevalent and complex post-translational modification	157:211	a prevalent and complex post-translational modification of milk proteins with significant biological importance	157:267	N-glycosylation is a prevalent and complex post-translational modification of milk proteins with significant biological importance.
36155291	5	82	gly	glycosites	811:820	arg1	glycoproteins					831:843	eight glycoproteins	825:843	eight glycoproteins	825:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	82	gly	glycosites	811:820	arg2	glycoproteins					831:843	eight glycoproteins	825:843	eight glycoproteins	825:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	5	82	gly	glycosites	811:820	arg2	glycosites					811:820	14 glycosites	808:820	14 glycosites of eight glycoproteins	808:843	Among them, 22 site-specific N-glycans attached to 14 glycosites of eight glycoproteins were significantly increased, whereas 30 site-specific N-glycans attached to 19 glycosites of 16 glycoproteins were significantly decreased.
36155291	4	83	theme	DC	684:685	arg1	N-glycoproteins					692:706	DC MFGM N-glycoproteins	684:706	DC MFGM N-glycoproteins	684:706	Mannosylation and fucosylation were predominant in DC MFGM N-glycoproteins compared to sialylation and mannosylation in DM.
36655103	0	0	theme	gut	84:86	arg1	microbiota					88:97	gut microbiota	84:97	gut microbiota	84:97	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	0	1	from	purification	14:25	arg1	microbiota					88:97	gut microbiota	84:97	gut microbiota	84:97	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	14	2	theme	buckwheat	1800:1808	arg1	utilization					1821:1831	Tartary buckwheat commercial utilization	1792:1831	Tartary buckwheat commercial utilization	1792:1831	They provide a basis for Tartary buckwheat commercial utilization for GM modulation.
36655103	11	3	theme	fecal	1431:1435	arg1	fermentation					1437:1448	fecal fermentation	1431:1448	fecal fermentation	1431:1448	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	4	4	theme	fractions	476:484	arg1	composition					455:465	The composition	451:465	The composition of these fractions	451:484	The composition of these fractions was determined using ion chromatography.
36655103	5	5	theme	Lactobacillus	642:654	arg1	species					612:618	three species	606:618	three species of Bifidobacteria and Lactobacillus rhamnosus	606:664	Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus.
36655103	11	6	theme	Phascolarctobacterium	1504:1524	arg1	abundance					1478:1486	an increased relative abundance	1456:1486	an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella	1456:1553	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	13	7	theme	dietary	1697:1703	arg1	sources					1720:1726	specific dietary polysaccharide sources	1688:1726	specific dietary polysaccharide sources	1688:1726	Our findings indicate that Tartary buckwheat can provide specific dietary polysaccharide sources to modulate and maintain GM diversity.
36655103	11	8	theme	increased	1459:1467	arg1	abundance					1478:1486	an increased relative abundance	1456:1486	an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella	1456:1553	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	1	9	theme	several	227:233	arg1	benefits					242:249	several health benefits	227:249	several health benefits	227:249	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	0	10	from	Isolation	0:8	arg1	microbiota					88:97	gut microbiota	84:97	gut microbiota	84:97	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	13	11	theme	specific	1688:1695	arg1	sources					1720:1726	specific dietary polysaccharide sources	1688:1726	specific dietary polysaccharide sources	1688:1726	Our findings indicate that Tartary buckwheat can provide specific dietary polysaccharide sources to modulate and maintain GM diversity.
36655103	1	12	from	polysaccharides	151:165	arg1	rich					143:146	rich	143:146	rich	143:146	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	11	13	theme	Lactococcus	1491:1501	arg1	abundance					1478:1486	an increased relative abundance	1456:1486	an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella	1456:1553	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	1	14	theme	health	235:240	arg1	benefits					242:249	several health benefits	227:249	several health benefits	227:249	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	11	15	theme	GM	1381:1382	arg1	composition					1366:1376	The composition	1362:1376	The composition of GM	1362:1382	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	8	16	attach	present	868:874	arg2	polysaccharides					852:866	The polysaccharides	848:866	The polysaccharides present in different fractions	848:897	The polysaccharides present in different fractions had specific probiotic effects.
36655103	8	16	attach	present	868:874	arg1	fractions					889:897	different fractions	879:897	different fractions	879:897	The polysaccharides present in different fractions had specific probiotic effects.
36655103	9	17	theme	rich	1068:1071	arg1	TBP-W					1061:1065	TBP-W	1061:1065	TBP-W	1061:1065	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	10	18	theme	breve	1348:1352	arg1	growth					1354:1359	Bifidobacterium breve growth	1332:1359	Bifidobacterium breve growth	1332:1359	Furthermore, TBP-0.2, composed of d-galacturonic acid, d-galactose, xylose, and arabinose, exhibited its highest impact on Bifidobacterium breve growth.
36655103	6	19	from	effect	684:689	arg1	GM					701:702	GM	701:702	GM	701:702	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	6	19	from	effect	684:689	arg1	SCFAs					733:737	SCFAs	733:737	SCFAs	733:737	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	6	19	from	effect	684:689	arg1	acids					726:730	short-chain fatty acids	708:730	short-chain fatty acids (SCFAs)	708:738	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	1	20	from	rich	143:146	arg1	polysaccharides					151:165	polysaccharides	151:165	polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits	151:249	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	6	21	theme	TBP	694:696	arg1	effect					684:689	the effect	680:689	the effect of TBP on GM and short-chain fatty acids (SCFAs)	680:738	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	0	22	theme	polysaccharides	48:62	arg1	purification					14:25	purification	14:25	purification	14:25	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	0	22	theme	polysaccharides	48:62	arg1	Isolation					0:8	Isolation	0:8	Isolation	0:8	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	0	22	theme	polysaccharides	48:62	arg1	effect					74:79	their effect	68:79	their effect on gut microbiota	68:97	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	13	23	theme	polysaccharide	1705:1718	arg1	sources					1720:1726	specific dietary polysaccharide sources	1688:1726	specific dietary polysaccharide sources	1688:1726	Our findings indicate that Tartary buckwheat can provide specific dietary polysaccharide sources to modulate and maintain GM diversity.
36655103	9	24	from	effect	1031:1036	arg1	rhamnosus					1044:1052	L. rhamnosus	1041:1052	L. rhamnosus	1041:1052	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	10	25	theme	d-galacturonic	1243:1256	arg1	acid					1258:1261	d-galacturonic acid	1243:1261	d-galacturonic acid	1243:1261	Furthermore, TBP-0.2, composed of d-galacturonic acid, d-galactose, xylose, and arabinose, exhibited its highest impact on Bifidobacterium breve growth.
36655103	8	26	contain	had	899:901	arg1	polysaccharides					852:866	The polysaccharides	848:866	The polysaccharides present in different fractions	848:897	The polysaccharides present in different fractions had specific probiotic effects.
36655103	8	26	contain	had	899:901	arg2	effects					922:928	specific probiotic effects	903:928	specific probiotic effects	903:928	The polysaccharides present in different fractions had specific probiotic effects.
36655103	13	27	theme	Tartary	1658:1664	arg1	buckwheat					1666:1674	Tartary buckwheat	1658:1674	Tartary buckwheat	1658:1674	Our findings indicate that Tartary buckwheat can provide specific dietary polysaccharide sources to modulate and maintain GM diversity.
36655103	9	28	theme	longum	1161:1166	arg1	growth					1201:1206	Bifidobacterium longum and Bifidobacterium adolescentis growth	1145:1206	Bifidobacterium longum and Bifidobacterium adolescentis growth	1145:1206	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	9	29	from	fructose	1102:1109	arg1	rich					1068:1071	rich	1068:1071	rich	1068:1071	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	9	30	theme	promoting	1125:1133	arg1	effect					1135:1140	the best promoting effect	1116:1140	the best promoting effect	1116:1140	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	5	31	theme	Bifidobacteria	623:636	arg1	species					612:618	three species	606:618	three species of Bifidobacteria and Lactobacillus rhamnosus	606:664	Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus.
36655103	11	32	theme	Bacteroidetes	1527:1539	arg1	abundance					1478:1486	an increased relative abundance	1456:1486	an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella	1456:1553	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	11	33	theme	Shigella	1546:1553	arg1	abundance					1478:1486	an increased relative abundance	1456:1486	an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella	1456:1553	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	7	34	theme	TBP	799:801	arg1	fraction					803:810	TBP fraction	799:810	TBP fraction	799:810	Results showed that the probiotic effect of TBP fraction was dependent on their composition.
36655103	13	35	theme	GM	1753:1754	arg1	diversity					1756:1764	GM diversity	1753:1764	GM diversity	1753:1764	Our findings indicate that Tartary buckwheat can provide specific dietary polysaccharide sources to modulate and maintain GM diversity.
36655103	7	36	theme	probiotic	779:787	arg1	dependent					816:824	dependent	816:824	dependent	816:824	Results showed that the probiotic effect of TBP fraction was dependent on their composition.
36655103	7	36	theme	probiotic	779:787	arg1	effect					789:794	the probiotic effect	775:794	the probiotic effect of TBP fraction	775:810	Results showed that the probiotic effect of TBP fraction was dependent on their composition.
36655103	10	37	theme	Bifidobacterium	1332:1346	arg1	growth					1354:1359	Bifidobacterium breve growth	1332:1359	Bifidobacterium breve growth	1332:1359	Furthermore, TBP-0.2, composed of d-galacturonic acid, d-galactose, xylose, and arabinose, exhibited its highest impact on Bifidobacterium breve growth.
36655103	1	38	theme	Tartary	100:106	arg1	buckwheat					108:116	Tartary buckwheat	100:116	Tartary buckwheat (Fagopyrum tataricum)	100:138	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	7	39	theme	fraction	803:810	arg1	dependent					816:824	dependent	816:824	dependent	816:824	Results showed that the probiotic effect of TBP fraction was dependent on their composition.
36655103	7	39	theme	fraction	803:810	arg1	effect					789:794	the probiotic effect	775:794	the probiotic effect of TBP fraction	775:810	Results showed that the probiotic effect of TBP fraction was dependent on their composition.
36655103	5	40	dep	Lactobacillus	642:654	arg1	rhamnosus					656:664	Lactobacillus rhamnosus	642:664	Lactobacillus rhamnosus	642:664	Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus.
36655103	5	41	theme	Different	527:535	arg1	components					541:550	Different TBP components	527:550	Different TBP components	527:550	Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus.
36655103	9	42	theme	adolescentis	1188:1199	arg1	growth					1201:1206	Bifidobacterium longum and Bifidobacterium adolescentis growth	1145:1206	Bifidobacterium longum and Bifidobacterium adolescentis growth	1145:1206	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	14	43	theme	commercial	1810:1819	arg1	utilization					1821:1831	Tartary buckwheat commercial utilization	1792:1831	Tartary buckwheat commercial utilization	1792:1831	They provide a basis for Tartary buckwheat commercial utilization for GM modulation.
36655103	14	44	theme	GM	1837:1838	arg1	modulation					1840:1849	GM modulation	1837:1849	GM modulation	1837:1849	They provide a basis for Tartary buckwheat commercial utilization for GM modulation.
36655103	0	45	theme	buckwheat	38:46	arg1	polysaccharides					48:62	Tartary buckwheat polysaccharides	30:62	Tartary buckwheat polysaccharides	30:62	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	12	46	theme	SCFA	1585:1588	arg1	level					1576:1580	the level	1572:1580	the level of SCFA	1572:1588	Simultaneously, the level of SCFA was also significantly increased by TBP.
36655103	8	47	theme	different	879:887	arg1	fractions					889:897	different fractions	879:897	different fractions	879:897	The polysaccharides present in different fractions had specific probiotic effects.
36655103	1	48	theme	gut	195:197	arg1	GM					211:212	GM	211:212	GM	211:212	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	1	48	theme	gut	195:197	arg1	microbiota					199:208	the gut microbiota	191:208	the gut microbiota (GM)	191:213	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	0	49	theme	Tartary	30:36	arg1	polysaccharides					48:62	Tartary buckwheat polysaccharides	30:62	Tartary buckwheat polysaccharides	30:62	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	0	50	from	effect	74:79	arg1	microbiota					88:97	gut microbiota	84:97	gut microbiota	84:97	Isolation and purification of Tartary buckwheat polysaccharides and their effect on gut microbiota.
36655103	9	51	from	glucose	1076:1082	arg1	rich					1068:1071	rich	1068:1071	rich	1068:1071	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	8	52	theme	specific	903:910	arg1	effects					922:928	specific probiotic effects	903:928	specific probiotic effects	903:928	The polysaccharides present in different fractions had specific probiotic effects.
36655103	9	53	from	d-galactose	1085:1095	arg1	rich					1068:1071	rich	1068:1071	rich	1068:1071	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	3	54	theme	Tartary	362:368	arg1	TBP					397:399	TBP	397:399	TBP	397:399	In this study, Tartary buckwheat polysaccharides (TBP) were purified, and five fractions were obtained.
36655103	3	54	theme	Tartary	362:368	arg1	polysaccharides					380:394	Tartary buckwheat polysaccharides	362:394	Tartary buckwheat polysaccharides (TBP)	362:400	In this study, Tartary buckwheat polysaccharides (TBP) were purified, and five fractions were obtained.
36655103	9	55	contain	had	1112:1114	arg1	TBP-W					1061:1065	TBP-W	1061:1065	TBP-W	1061:1065	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	9	55	contain	had	1112:1114	arg2	effect					1135:1140	the best promoting effect	1116:1140	the best promoting effect	1116:1140	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	14	56	theme	Tartary	1792:1798	arg1	utilization					1821:1831	Tartary buckwheat commercial utilization	1792:1831	Tartary buckwheat commercial utilization	1792:1831	They provide a basis for Tartary buckwheat commercial utilization for GM modulation.
36655103	3	57	theme	buckwheat	370:378	arg1	TBP					397:399	TBP	397:399	TBP	397:399	In this study, Tartary buckwheat polysaccharides (TBP) were purified, and five fractions were obtained.
36655103	3	57	theme	buckwheat	370:378	arg1	polysaccharides					380:394	Tartary buckwheat polysaccharides	362:394	Tartary buckwheat polysaccharides (TBP)	362:400	In this study, Tartary buckwheat polysaccharides (TBP) were purified, and five fractions were obtained.
36655103	4	58	theme	ion	507:509	arg1	chromatography					511:524	ion chromatography	507:524	ion chromatography	507:524	The composition of these fractions was determined using ion chromatography.
36655103	9	59	from	rich	1068:1071	arg1	d-galactose					1085:1095	d-galactose	1085:1095	d-galactose	1085:1095	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	9	59	from	rich	1068:1071	arg1	fructose					1102:1109	fructose	1102:1109	fructose	1102:1109	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	9	59	from	rich	1068:1071	arg1	glucose					1076:1082	glucose	1076:1082	glucose	1076:1082	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	5	60	theme	probiotic	586:594	arg1	effect					596:601	their probiotic effect	580:601	their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus	580:664	Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus.
36655103	2	61	theme	polysaccharides	307:321	arg1	action					291:296	the action	287:296	the action of these polysaccharides	287:321	However, the mechanisms underlying the action of these polysaccharides remain unclear to date.
36655103	8	62	from	fractions	889:897	arg1	present					868:874	present	868:874	present	868:874	The polysaccharides present in different fractions had specific probiotic effects.
36655103	1	63	used	utilized	179:186	arg2	polysaccharides					151:165	polysaccharides	151:165	polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits	151:249	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	5	64	theme	TBP	537:539	arg1	components					541:550	Different TBP components	527:550	Different TBP components	527:550	Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus.
36655103	8	65	theme	probiotic	912:920	arg1	effects					922:928	specific probiotic effects	903:928	specific probiotic effects	903:928	The polysaccharides present in different fractions had specific probiotic effects.
36655103	6	66	theme	fatty	720:724	arg1	SCFAs					733:737	SCFAs	733:737	SCFAs	733:737	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	6	66	theme	fatty	720:724	arg1	acids					726:730	short-chain fatty acids	708:730	short-chain fatty acids (SCFAs)	708:738	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	8	67	theme	present	868:874	arg1	polysaccharides					852:866	The polysaccharides	848:866	The polysaccharides present in different fractions	848:897	The polysaccharides present in different fractions had specific probiotic effects.
36655103	10	68	from	impact	1322:1327	arg1	growth					1354:1359	Bifidobacterium breve growth	1332:1359	Bifidobacterium breve growth	1332:1359	Furthermore, TBP-0.2, composed of d-galacturonic acid, d-galactose, xylose, and arabinose, exhibited its highest impact on Bifidobacterium breve growth.
36655103	11	69	theme	relative	1469:1476	arg1	abundance					1478:1486	an increased relative abundance	1456:1486	an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella	1456:1553	The composition of GM was significantly altered by adding TBP during fecal fermentation, with an increased relative abundance of Lactococcus, Phascolarctobacterium, Bacteroidetes, and Shigella.
36655103	6	70	theme	short-chain	708:718	arg1	SCFAs					733:737	SCFAs	733:737	SCFAs	733:737	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	6	70	theme	short-chain	708:718	arg1	acids					726:730	short-chain fatty acids	708:730	short-chain fatty acids (SCFAs)	708:738	In addition, the effect of TBP on GM and short-chain fatty acids (SCFAs) was evaluated.
36655103	5	71	from	effect	596:601	arg1	species					612:618	three species	606:618	three species of Bifidobacteria and Lactobacillus rhamnosus	606:664	Different TBP components were investigated regarding their probiotic effect on three species of Bifidobacteria and Lactobacillus rhamnosus.
36655103	9	72	theme	strongest	1007:1015	arg1	effect					1031:1036	the strongest proliferation effect	1003:1036	the strongest proliferation effect on L. rhamnosus	1003:1052	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36655103	8	73	from	present	868:874	arg1	fractions					889:897	different fractions	879:897	different fractions	879:897	The polysaccharides present in different fractions had specific probiotic effects.
36655103	10	74	theme	highest	1314:1320	arg1	impact					1322:1327	its highest impact	1310:1327	its highest impact on Bifidobacterium breve growth	1310:1359	Furthermore, TBP-0.2, composed of d-galacturonic acid, d-galactose, xylose, and arabinose, exhibited its highest impact on Bifidobacterium breve growth.
36655103	1	75	dep	buckwheat	108:116	arg1	tataricum					129:137	Fagopyrum tataricum	119:137	Fagopyrum tataricum	119:137	Tartary buckwheat (Fagopyrum tataricum) is rich in polysaccharides that can be utilized by the gut microbiota (GM) and provide several health benefits.
36655103	9	76	theme	proliferation	1017:1029	arg1	effect					1031:1036	the strongest proliferation effect	1003:1036	the strongest proliferation effect on L. rhamnosus	1003:1052	TBP-1.0, mainly composed of fucose, glucose, and d-galactose, exhibited the strongest proliferation effect on L. rhamnosus, while TBP-W, rich in glucose, d-galactose, and fructose, had the best promoting effect on Bifidobacterium longum and Bifidobacterium adolescentis growth.
36555773	9	0	theme	Atractylodis	2444:2455	arg1	candidate					2485:2493	a potential drug candidate	2468:2493	a potential drug candidate for the treatment of ALI	2468:2518	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	9	0	theme	Atractylodis	2444:2455	arg1	rhizoma					2457:2463	Atractylodis rhizoma	2444:2463	Atractylodis rhizoma	2444:2463	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	8	1	theme	targets	2162:2168	arg1	expression					2124:2133	the expression	2120:2133	the expression of Nrf2 and its downstream targets HO-1	2120:2173	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	1	theme	targets	2162:2168	arg1	NQO-1					2179:2183	NQO-1	2179:2183	NQO-1	2179:2183	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	1	theme	targets	2162:2168	arg1	mechanism					2200:2208	the molecular mechanism	2186:2208	the molecular mechanism of which was consistent with in vivo observations	2186:2258	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	9	2	theme	LPS-induced	2411:2421	arg1	ALI					2423:2425	LPS-induced ALI	2411:2425	LPS-induced ALI	2411:2425	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	8	3	theme	pathway	2009:2015	arg1	inhibition					1975:1984	inhibition	1975:1984	inhibition of the NF-κB signaling pathway	1975:2015	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	6	4	theme	factors	1392:1398	arg1	expression					1365:1374	the expression	1361:1374	the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2))	1361:1538	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	9	5	theme	potential	2470:2478	arg1	candidate					2485:2493	a potential drug candidate	2468:2493	a potential drug candidate for the treatment of ALI	2468:2518	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	9	5	theme	potential	2470:2478	arg1	rhizoma					2457:2463	Atractylodis rhizoma	2444:2463	Atractylodis rhizoma	2444:2463	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	4	6	theme	performance	823:833	arg1	HPLC					858:861	HPLC	858:861	HPLC	858:861	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	4	6	theme	performance	823:833	arg1	chromatography					842:855	high performance liquid chromatography	818:855	high performance liquid chromatography (HPLC)	818:862	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	5	7	theme	superoxide	1129:1138	arg1	SOD					1151:1153	SOD	1151:1153	SOD	1151:1153	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	5	7	theme	superoxide	1129:1138	arg1	dismutase					1140:1148	superoxide dismutase	1129:1148	superoxide dismutase (SOD)	1129:1154	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	7	8	dep	proteins	1801:1808	arg1	NQO-1					1879:1883	NQO-1	1879:1883	NQO-1	1879:1883	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	8	dep	proteins	1801:1808	arg1	proteins					1801:1808	its downstream proteins	1786:1808	its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1786:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	8	dep	proteins	1801:1808	arg1	oxidoreductase					1861:1874	NADPH quinone acceptor oxidoreductase 1	1838:1876	NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1838:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	8	dep	proteins	1801:1808	arg1	HO-1					1828:1831	HO-1	1828:1831	HO-1	1828:1831	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	8	dep	proteins	1801:1808	arg1	oxygenase-1					1815:1825	heme oxygenase-1	1810:1825	heme oxygenase-1 (HO-1)	1810:1832	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	9	theme	acceptor	1852:1859	arg1	NQO-1					1879:1883	NQO-1	1879:1883	NQO-1	1879:1883	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	9	theme	acceptor	1852:1859	arg1	proteins					1801:1808	its downstream proteins	1786:1808	its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1786:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	9	theme	acceptor	1852:1859	arg1	oxidoreductase					1861:1874	NADPH quinone acceptor oxidoreductase 1	1838:1876	NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1838:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	8	10	theme	molecular	2190:2198	arg1	NQO-1					2179:2183	NQO-1	2179:2183	NQO-1	2179:2183	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	10	theme	molecular	2190:2198	arg1	mechanism					2200:2208	the molecular mechanism	2186:2208	the molecular mechanism of which was consistent with in vivo observations	2186:2258	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	3	11	theme	Atractylodis	576:587	arg1	rhizoma					589:595	Atractylodis rhizoma	576:595	Atractylodis rhizoma for the treatment of ALI	576:620	However, few studies of Atractylodis rhizoma for the treatment of ALI have been published, and its mechanism of action remains unclear.
36555773	7	12	theme	NADPH	1838:1842	arg1	NQO-1					1879:1883	NQO-1	1879:1883	NQO-1	1879:1883	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	12	theme	NADPH	1838:1842	arg1	proteins					1801:1808	its downstream proteins	1786:1808	its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1786:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	12	theme	NADPH	1838:1842	arg1	oxidoreductase					1861:1874	NADPH quinone acceptor oxidoreductase 1	1838:1876	NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1838:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	8	13	theme	-induced	2066:2073	arg1	stress					2085:2090	lipopolysaccharide (LPS)-induced oxidative stress	2042:2090	lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells	2042:2105	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	5	14	theme	lung	1002:1005	arg1	W/D					1016:1018	W/D	1016:1018	W/D	1016:1018	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	5	14	theme	lung	1002:1005	arg1	wet/dry					1007:1013	the lung wet/dry	998:1013	the lung wet/dry (W/D) ratio	998:1025	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	8	15	theme	THP-1	2095:2099	arg1	cells					2101:2105	THP-1 cells	2095:2105	THP-1 cells	2095:2105	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	6	16	theme	nitric	1480:1485	arg1	synthase					1493:1500	inducible nitric oxide synthase	1470:1500	inducible nitric oxide synthase (INOS)	1470:1507	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	6	16	theme	nitric	1480:1485	arg1	INOS					1503:1506	INOS	1503:1506	INOS	1503:1506	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	4	17	theme	-induced	932:939	arg1	model					949:953	a lipopolysaccharide (LPS)-induced ALI rat model	906:953	a lipopolysaccharide (LPS)-induced ALI rat model	906:953	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	6	18	theme	EEAR	1297:1300	arg1	changes					1342:1348	EEAR significantly reduced histopathological changes	1297:1348	EEAR significantly reduced histopathological changes	1297:1348	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	3	19	theme	ALI	618:620	arg1	treatment					605:613	the treatment	601:613	the treatment of ALI	601:620	However, few studies of Atractylodis rhizoma for the treatment of ALI have been published, and its mechanism of action remains unclear.
36555773	4	20	theme	rat	945:947	arg1	model					949:953	a lipopolysaccharide (LPS)-induced ALI rat model	906:953	a lipopolysaccharide (LPS)-induced ALI rat model	906:953	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	1	21	theme	Acute	169:173	arg1	ALI					188:190	ALI	188:190	ALI	188:190	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	1	21	theme	Acute	169:173	arg1	injury					180:185	Acute lung injury	169:185	Acute lung injury (ALI)	169:191	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	1	21	theme	Acute	169:173	arg1	syndrome					198:205	a syndrome	196:205	a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking	196:373	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	2	22	theme	cellular	534:541	arg1	barrier					543:549	the cellular barrier	530:549	the cellular barrier	530:549	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	1	23	dep	hypoxemia	281:289	arg1	both					291:294	both	291:294	both	291:294	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	4	24	theme	extract	752:758	arg1	composition					723:733	the chemical composition	710:733	the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR)	710:789	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	1	25	theme	therapeutic	345:355	arg1	drugs					357:361	effective therapeutic drugs	335:361	effective therapeutic drugs	335:361	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	7	26	theme	proteins	1801:1808	arg1	expression					1717:1726	the expression	1713:1726	the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1713:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	8	27	from	stress	2085:2090	arg1	damage					2030:2035	damage	2030:2035	damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells	2030:2105	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	27	from	stress	2085:2090	arg1	cells					2101:2105	THP-1 cells	2095:2105	THP-1 cells	2095:2105	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	4	28	theme	Atractylodis	763:774	arg1	EEAR					785:788	EEAR	785:788	EEAR	785:788	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	4	28	theme	Atractylodis	763:774	arg1	rhizoma					776:782	Atractylodis rhizoma	763:782	Atractylodis rhizoma (EEAR)	763:789	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	0	29	theme	Keap1/Nrf2	117:126	arg1	Pathways					138:145	Keap1/Nrf2 Signaling Pathways	117:145	Keap1/Nrf2 Signaling Pathways	117:145	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	5	30	with	rats	1191:1194	arg1	ALI					1201:1203	ALI	1201:1203	ALI	1201:1203	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	7	31	from	stress	1683:1688	arg1	ALI					1693:1695	ALI	1693:1695	ALI	1693:1695	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	2	32	theme	anti-inflammatory	444:460	arg1	properties					476:485	excellent anti-inflammatory and antiviral properties	434:485	excellent anti-inflammatory and antiviral properties	434:485	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	6	33	theme	signaling	1583:1591	arg1	pathway					1593:1599	the NF-κB signaling pathway	1573:1599	the NF-κB signaling pathway	1573:1599	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	2	34	theme	Atractylodis	376:387	arg1	rhizoma					389:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	2	34	theme	Atractylodis	376:387	arg1	medicine					420:427	a traditional Chinese medicine	398:427	a traditional Chinese medicine	398:427	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	0	35	theme	Protective	0:9	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.	0:167	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	1	36	theme	intractable	269:279	arg1	hypoxemia					281:289	intractable hypoxemia	269:289	intractable hypoxemia	269:289	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	8	37	theme	factor	1930:1935	arg1	expression					1937:1946	LPS-induced inflammatory factor expression	1905:1946	LPS-induced inflammatory factor expression in THP-1 cells	1905:1961	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	9	38	theme	signaling	2379:2387	arg1	pathways					2389:2396	TLR4/NF-κB and Keap1/Nrf2 signaling pathways	2353:2396	TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI	2353:2425	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	5	39	theme	barrier	1229:1235	arg1	function					1237:1244	lung barrier function	1224:1244	lung barrier function	1224:1244	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	2	40	theme	traditional	400:410	arg1	rhizoma					389:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	2	40	theme	traditional	400:410	arg1	medicine					420:427	a traditional Chinese medicine	398:427	a traditional Chinese medicine	398:427	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	0	41	theme	lancea	35:40	arg1	Rhizoma					42:48	Atractylodis lancea Rhizoma	22:48	Atractylodis lancea Rhizoma	22:48	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	5	42	theme	myeloperoxidase	1083:1097	arg1	formation					1105:1113	malondialdehyde (MDA) and myeloperoxidase (MPO) formation	1057:1113	malondialdehyde (MDA) and myeloperoxidase (MPO) formation	1057:1113	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	7	43	theme	factor	1766:1771	arg1	expression					1717:1726	the expression	1713:1726	the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1713:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	6	44	dep	factors	1392:1398	arg1	such					1401:1404	such	1401:1404	such	1401:1404	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	2	45	theme	antiviral	466:474	arg1	properties					476:485	excellent anti-inflammatory and antiviral properties	434:485	excellent anti-inflammatory and antiviral properties	434:485	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	0	46	theme	Acute	80:84	arg1	Injury					91:96	Lipopolysaccharide-Induced Acute Lung Injury	53:96	Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo	53:166	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	6	47	theme	reduced	1316:1322	arg1	changes					1342:1348	EEAR significantly reduced histopathological changes	1297:1348	EEAR significantly reduced histopathological changes	1297:1348	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	7	48	theme	erythroid	1746:1754	arg1	factor					1766:1771	nuclear factor erythroid 2-related factor 2	1731:1773	nuclear factor erythroid 2-related factor 2 (Nrf2)	1731:1780	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	48	theme	erythroid	1746:1754	arg1	Nrf2					1776:1779	Nrf2	1776:1779	Nrf2	1776:1779	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	6	49	theme	necrosis	1415:1422	arg1	TNF-α					1434:1438	TNF-α	1434:1438	TNF-α	1434:1438	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	6	49	theme	necrosis	1415:1422	arg1	factor-α					1424:1431	tumor necrosis factor-α	1409:1431	tumor necrosis factor-α (TNF-α)	1409:1439	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	7	50	theme	nuclear	1731:1737	arg1	factor					1766:1771	nuclear factor erythroid 2-related factor 2	1731:1773	nuclear factor erythroid 2-related factor 2 (Nrf2)	1731:1780	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	50	theme	nuclear	1731:1737	arg1	Nrf2					1776:1779	Nrf2	1776:1779	Nrf2	1776:1779	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	8	51	theme	downstream	2151:2160	arg1	targets					2162:2168	its downstream targets	2147:2168	its downstream targets	2147:2168	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	52	from	expression	1937:1946	arg1	cells					1957:1961	THP-1 cells	1951:1961	THP-1 cells	1951:1961	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	5	53	with	Treatment	956:964	arg1	EEAR					971:974	EEAR	971:974	EEAR	971:974	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	5	54	theme	glutathione	1160:1170	arg1	depletion					1178:1186	glutathione (GSH) depletion	1160:1186	glutathione (GSH) depletion	1160:1186	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	6	55	theme	histopathological	1324:1340	arg1	changes					1342:1348	EEAR significantly reduced histopathological changes	1297:1348	EEAR significantly reduced histopathological changes	1297:1348	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	7	56	theme	oxidative	1673:1681	arg1	stress					1683:1688	oxidative stress	1673:1688	oxidative stress in ALI	1673:1695	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	1	57	theme	inflammatory	230:241	arg1	response					243:250	an excessive inflammatory response	217:250	an excessive inflammatory response characterized by intractable hypoxemia both inside	217:301	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	8	58	theme	signaling	1999:2007	arg1	pathway					2009:2015	the NF-κB signaling pathway	1989:2015	the NF-κB signaling pathway	1989:2015	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	9	59	theme	oxidative	2305:2313	arg1	stress					2315:2320	oxidative stress	2305:2320	oxidative stress	2305:2320	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	2	60	contain	has	430:432	arg1	rhizoma					389:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	2	60	contain	has	430:432	arg2	properties					476:485	excellent anti-inflammatory and antiviral properties	434:485	excellent anti-inflammatory and antiviral properties	434:485	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	2	60	contain	has	430:432	arg1	medicine					420:427	a traditional Chinese medicine	398:427	a traditional Chinese medicine	398:427	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	6	61	theme	inflammatory	1379:1390	arg1	factors					1392:1398	inflammatory factors	1379:1398	inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2))	1379:1538	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	1	62	theme	lung	175:178	arg1	ALI					188:190	ALI	188:190	ALI	188:190	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	1	62	theme	lung	175:178	arg1	injury					180:185	Acute lung injury	169:185	Acute lung injury (ALI)	169:191	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	1	62	theme	lung	175:178	arg1	syndrome					198:205	a syndrome	196:205	a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking	196:373	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	9	63	theme	drug	2480:2483	arg1	candidate					2485:2493	a potential drug candidate	2468:2493	a potential drug candidate for the treatment of ALI	2468:2518	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	9	63	theme	drug	2480:2483	arg1	rhizoma					2457:2463	Atractylodis rhizoma	2444:2463	Atractylodis rhizoma	2444:2463	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	8	64	theme	mechanism	2200:2208	arg1	consistent					2223:2232	consistent	2223:2232	consistent	2223:2232	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	4	65	theme	high	818:821	arg1	HPLC					858:861	HPLC	858:861	HPLC	858:861	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	4	65	theme	high	818:821	arg1	chromatography					842:855	high performance liquid chromatography	818:855	high performance liquid chromatography (HPLC)	818:862	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	6	66	theme	interleukin-1	1442:1454	arg1	beta					1456:1459	interleukin-1 beta	1442:1459	interleukin-1 beta (IL-1β)	1442:1467	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	6	66	theme	interleukin-1	1442:1454	arg1	IL-1β					1462:1466	IL-1β	1462:1466	IL-1β	1462:1466	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	4	67	theme	liquid	835:840	arg1	HPLC					858:861	HPLC	858:861	HPLC	858:861	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	4	67	theme	liquid	835:840	arg1	chromatography					842:855	high performance liquid chromatography	818:855	high performance liquid chromatography (HPLC)	818:862	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	3	68	theme	few	561:563	arg1	studies					565:571	few studies	561:571	few studies of Atractylodis rhizoma for the treatment of ALI	561:620	However, few studies of Atractylodis rhizoma for the treatment of ALI have been published, and its mechanism of action remains unclear.
36555773	7	69	theme	quinone	1844:1850	arg1	NQO-1					1879:1883	NQO-1	1879:1883	NQO-1	1879:1883	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	69	theme	quinone	1844:1850	arg1	proteins					1801:1808	its downstream proteins	1786:1808	its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1786:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	69	theme	quinone	1844:1850	arg1	oxidoreductase					1861:1874	NADPH quinone acceptor oxidoreductase 1	1838:1876	NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1838:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	6	70	theme	NF-κB	1577:1581	arg1	pathway					1593:1599	the NF-κB signaling pathway	1573:1599	the NF-κB signaling pathway	1573:1599	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	8	71	dep	Nrf2	2138:2141	arg1	HO-1					2170:2173	HO-1	2170:2173	HO-1	2170:2173	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	9	72	theme	ALI	2516:2518	arg1	treatment					2503:2511	the treatment	2499:2511	the treatment of ALI	2499:2518	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	8	73	theme	in	2239:2240	arg1	observations					2247:2258	in vivo observations	2239:2258	in vivo observations	2239:2258	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	2	74	theme	barrier	543:549	arg1	integrity					517:525	the integrity	513:525	the integrity of the cellular barrier	513:549	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	5	75	theme	neutrophil	1028:1037	arg1	infiltration					1039:1050	neutrophil infiltration	1028:1050	neutrophil infiltration	1028:1050	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	3	76	theme	rhizoma	589:595	arg1	studies					565:571	few studies	561:571	few studies of Atractylodis rhizoma for the treatment of ALI	561:620	However, few studies of Atractylodis rhizoma for the treatment of ALI have been published, and its mechanism of action remains unclear.
36555773	8	77	theme	oxidative	2075:2083	arg1	stress					2085:2090	lipopolysaccharide (LPS)-induced oxidative stress	2042:2090	lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells	2042:2105	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	4	78	theme	present	695:701	arg1	study					703:707	the present study	691:707	the present study	691:707	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	5	79	theme	wet/dry	1007:1013	arg1	ratio					1021:1025	the lung wet/dry (W/D) ratio	998:1025	the lung wet/dry (W/D) ratio	998:1025	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	6	80	theme	oxide	1487:1491	arg1	synthase					1493:1500	inducible nitric oxide synthase	1470:1500	inducible nitric oxide synthase (INOS)	1470:1507	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	6	80	theme	oxide	1487:1491	arg1	INOS					1503:1506	INOS	1503:1506	INOS	1503:1506	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	0	81	theme	Lung	86:89	arg1	Injury					91:96	Lipopolysaccharide-Induced Acute Lung Injury	53:96	Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo	53:166	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	6	82	theme	inducible	1470:1478	arg1	synthase					1493:1500	inducible nitric oxide synthase	1470:1500	inducible nitric oxide synthase (INOS)	1470:1507	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	6	82	theme	inducible	1470:1478	arg1	INOS					1503:1506	INOS	1503:1506	INOS	1503:1506	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	5	83	theme	GSH	1173:1175	arg1	depletion					1178:1186	glutathione (GSH) depletion	1160:1186	glutathione (GSH) depletion	1160:1186	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	7	84	theme	heme	1810:1813	arg1	proteins					1801:1808	its downstream proteins	1786:1808	its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1786:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	84	theme	heme	1810:1813	arg1	HO-1					1828:1831	HO-1	1828:1831	HO-1	1828:1831	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	84	theme	heme	1810:1813	arg1	oxygenase-1					1815:1825	heme oxygenase-1	1810:1825	heme oxygenase-1 (HO-1)	1810:1832	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	2	85	dep	protecting	502:511	arg1	addition					490:497	addition	490:497	addition	490:497	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	4	86	theme	ALI	941:943	arg1	model					949:953	a lipopolysaccharide (LPS)-induced ALI rat model	906:953	a lipopolysaccharide (LPS)-induced ALI rat model	906:953	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	0	87	from	Effects	11:17	arg1	Injury					91:96	Lipopolysaccharide-Induced Acute Lung Injury	53:96	Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo	53:166	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	9	88	theme	inflammatory	2326:2337	arg1	responses					2339:2347	inflammatory responses	2326:2347	inflammatory responses	2326:2347	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	1	89	theme	effective	335:343	arg1	drugs					357:361	effective therapeutic drugs	335:361	effective therapeutic drugs	335:361	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36555773	7	90	theme	downstream	1790:1799	arg1	proteins					1801:1808	its downstream proteins	1786:1808	its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1786:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	90	theme	downstream	1790:1799	arg1	oxidoreductase					1861:1874	NADPH quinone acceptor oxidoreductase 1	1838:1876	NADPH quinone acceptor oxidoreductase 1 (NQO-1)	1838:1884	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	90	theme	downstream	1790:1799	arg1	oxygenase-1					1815:1825	heme oxygenase-1	1810:1825	heme oxygenase-1 (HO-1)	1810:1832	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	4	91	theme	ethanolic	742:750	arg1	extract					752:758	the ethanolic extract	738:758	the ethanolic extract of Atractylodis rhizoma (EEAR)	738:789	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	0	92	theme	Signaling	128:136	arg1	Pathways					138:145	Keap1/Nrf2 Signaling Pathways	117:145	Keap1/Nrf2 Signaling Pathways	117:145	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	8	93	theme	inflammatory	1917:1928	arg1	expression					1937:1946	LPS-induced inflammatory factor expression	1905:1946	LPS-induced inflammatory factor expression in THP-1 cells	1905:1961	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	6	94	theme	pathway	1593:1599	arg1	activation					1559:1568	the activation	1555:1568	the activation of the NF-κB signaling pathway	1555:1599	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	5	95	theme	malondialdehyde	1057:1071	arg1	formation					1105:1113	malondialdehyde (MDA) and myeloperoxidase (MPO) formation	1057:1113	malondialdehyde (MDA) and myeloperoxidase (MPO) formation	1057:1113	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	9	96	theme	TLR4/NF-κB	2353:2362	arg1	pathways					2389:2396	TLR4/NF-κB and Keap1/Nrf2 signaling pathways	2353:2396	TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI	2353:2425	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	9	97	dep	pathways	2389:2396	arg1	alleviate					2401:2409	alleviate	2401:2409	to alleviate LPS-induced ALI	2398:2425	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	2	98	theme	excellent	434:442	arg1	properties					476:485	excellent anti-inflammatory and antiviral properties	434:485	excellent anti-inflammatory and antiviral properties	434:485	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	4	99	theme	chemical	714:721	arg1	composition					723:733	the chemical composition	710:733	the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR)	710:789	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	0	100	theme	Atractylodis	22:33	arg1	Rhizoma					42:48	Atractylodis lancea Rhizoma	22:48	Atractylodis lancea Rhizoma	22:48	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	9	101	theme	Keap1/Nrf2	2368:2377	arg1	pathways					2389:2396	TLR4/NF-κB and Keap1/Nrf2 signaling pathways	2353:2396	TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI	2353:2425	Therefore, we conclude that EEAR attenuates oxidative stress and inflammatory responses via TLR4/NF-κB and Keap1/Nrf2 signaling pathways to alleviate LPS-induced ALI, suggesting that Atractylodis rhizoma is a potential drug candidate for the treatment of ALI.
36555773	8	102	theme	THP-1	1951:1955	arg1	cells					1957:1961	THP-1 cells	1951:1961	THP-1 cells	1951:1961	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	5	103	theme	lung	1224:1227	arg1	function					1237:1244	lung barrier function	1224:1244	lung barrier function	1224:1244	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	2	104	theme	Chinese	412:418	arg1	rhizoma					389:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma	376:395	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	2	104	theme	Chinese	412:418	arg1	medicine					420:427	a traditional Chinese medicine	398:427	a traditional Chinese medicine	398:427	Atractylodis rhizoma, a traditional Chinese medicine, has excellent anti-inflammatory and antiviral properties in addition to protecting the integrity of the cellular barrier.
36555773	0	105	theme	Rhizoma	42:48	arg1	Effects					11:17	Protective Effects	0:17	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.	0:167	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	7	106	theme	2-related	1756:1764	arg1	factor					1766:1771	nuclear factor erythroid 2-related factor 2	1731:1773	nuclear factor erythroid 2-related factor 2 (Nrf2)	1731:1780	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	106	theme	2-related	1756:1764	arg1	Nrf2					1776:1779	Nrf2	1776:1779	Nrf2	1776:1779	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	8	107	with	consistent	2223:2232	arg1	observations					2247:2258	in vivo observations	2239:2258	in vivo observations	2239:2258	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	108	dep	in	2239:2240	arg1	vivo					2242:2245	vivo	2242:2245	vivo	2242:2245	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	0	109	theme	Lipopolysaccharide-Induced	53:78	arg1	Injury					91:96	Lipopolysaccharide-Induced Acute Lung Injury	53:96	Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo	53:166	Protective Effects of Atractylodis lancea Rhizoma on Lipopolysaccharide-Induced Acute Lung Injury via TLR4/NF-κB and Keap1/Nrf2 Signaling Pathways In Vitro and In Vivo.
36555773	7	110	theme	factor	1739:1744	arg1	factor					1766:1771	nuclear factor erythroid 2-related factor 2	1731:1773	nuclear factor erythroid 2-related factor 2 (Nrf2)	1731:1780	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	110	theme	factor	1739:1744	arg1	Nrf2					1776:1779	Nrf2	1776:1779	Nrf2	1776:1779	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	111	located	found	1652:1656	arg1	addition					1633:1640	addition	1633:1640	addition	1633:1640	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	7	111	located	found	1652:1656	arg2	EEAR					1643:1646	EEAR	1643:1646	EEAR	1643:1646	In addition, EEAR was found to also reduce oxidative stress in ALI by upregulating the expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream proteins heme oxygenase-1 (HO-1) and NADPH quinone acceptor oxidoreductase 1 (NQO-1).
36555773	3	112	theme	action	664:669	arg1	mechanism					651:659	its mechanism	647:659	its mechanism of action	647:669	However, few studies of Atractylodis rhizoma for the treatment of ALI have been published, and its mechanism of action remains unclear.
36555773	8	113	theme	Nrf2	2138:2141	arg1	expression					2124:2133	the expression	2120:2133	the expression of Nrf2 and its downstream targets HO-1	2120:2173	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	113	theme	Nrf2	2138:2141	arg1	NQO-1					2179:2183	NQO-1	2179:2183	NQO-1	2179:2183	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	113	theme	Nrf2	2138:2141	arg1	mechanism					2200:2208	the molecular mechanism	2186:2208	the molecular mechanism of which was consistent with in vivo observations	2186:2258	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	4	114	theme	rhizoma	776:782	arg1	extract					752:758	the ethanolic extract	738:758	the ethanolic extract of Atractylodis rhizoma (EEAR)	738:789	In the present study, the chemical composition of the ethanolic extract of Atractylodis rhizoma (EEAR) was initially clarified by high performance liquid chromatography (HPLC), after which it was studied in vivo using a lipopolysaccharide (LPS)-induced ALI rat model.
36555773	5	115	theme	lung	1271:1274	arg1	injury					1276:1281	lung injury	1271:1281	lung injury	1271:1281	Treatment with EEAR significantly reduced the lung wet/dry (W/D) ratio, neutrophil infiltration, and malondialdehyde (MDA) and myeloperoxidase (MPO) formation, and enhanced superoxide dismutase (SOD) and glutathione (GSH) depletion in rats with ALI, thereby improving lung barrier function and effectively reducing lung injury.
36555773	6	116	theme	tumor	1409:1413	arg1	TNF-α					1434:1438	TNF-α	1434:1438	TNF-α	1434:1438	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	6	116	theme	tumor	1409:1413	arg1	factor-α					1424:1431	tumor necrosis factor-α	1409:1431	tumor necrosis factor-α (TNF-α)	1409:1439	In addition, EEAR significantly reduced histopathological changes, decreased the expression of inflammatory factors (such as tumor necrosis factor-α (TNF-α), interleukin-1 beta (IL-1β), inducible nitric oxide synthase (INOS), and cyclooxygenase-2 (COX-2)), and inhibited the activation of the NF-κB signaling pathway, thus reducing inflammation.
36555773	8	117	theme	NF-κB	1993:1997	arg1	pathway					2009:2015	the NF-κB signaling pathway	1989:2015	the NF-κB signaling pathway	1989:2015	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	8	118	theme	LPS-induced	1905:1915	arg1	expression					1937:1946	LPS-induced inflammatory factor expression	1905:1946	LPS-induced inflammatory factor expression in THP-1 cells	1905:1961	EEAR also reduced LPS-induced inflammatory factor expression in THP-1 cells in vitro by inhibition of the NF-κB signaling pathway, and reduced damage from lipopolysaccharide (LPS)-induced oxidative stress in THP-1 cells by promoting the expression of Nrf2 and its downstream targets HO-1 and NQO-1, the molecular mechanism of which was consistent with in vivo observations.
36555773	1	119	theme	excessive	220:228	arg1	response					243:250	an excessive inflammatory response	217:250	an excessive inflammatory response characterized by intractable hypoxemia both inside	217:301	Acute lung injury (ALI) is a syndrome caused by an excessive inflammatory response characterized by intractable hypoxemia both inside and outside the lung, for which effective therapeutic drugs are lacking.
36232510	7	0	dep	fraction	1045:1052	arg1	FRBE					1064:1067	FRBE	1064:1067	FRBE	1064:1067	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	7	0	dep	fraction	1045:1052	arg1	FRBF					1055:1058	FRBF	1055:1058	FRBF	1055:1058	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	10	1	theme	hydrocarbon	1500:1510	arg1	receptor					1512:1519	the aryl hydrocarbon receptor	1491:1519	the aryl hydrocarbon receptor	1491:1519	Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor.
36232510	5	2	theme	rice	656:659	arg1	fractions					666:674	The fermented rice bran fractions	642:674	The fermented rice bran fractions	642:674	The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity.
36232510	3	3	theme	key	498:500	arg1	role					502:505	a key role	496:505	a key role	496:505	This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function.
36232510	6	4	theme	LPS-stimulated	973:986	arg1	cells					998:1002	LPS-stimulated RAW 264.7 cells	973:1002	LPS-stimulated RAW 264.7 cells	973:1002	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	0	5	theme	Lipopolysaccharide-Induced	69:94	arg1	Inflammation					96:107	Lipopolysaccharide-Induced Inflammation	69:107	Lipopolysaccharide-Induced Inflammation	69:107	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	5	6	theme	bran	661:664	arg1	fractions					666:674	The fermented rice bran fractions	642:674	The fermented rice bran fractions	642:674	The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity.
36232510	8	7	from	extracts	1262:1269	arg1	present					1245:1251	present	1245:1251	present	1245:1251	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	9	8	theme	rice	1384:1387	arg1	bran					1389:1392	fermented rice bran	1374:1392	fermented rice bran	1374:1392	These results indicate that tryptamine may play an important role in the anti-inflammatory effects of fermented rice bran.
36232510	5	9	theme	murine	732:737	arg1	macrophages					739:749	lipopolysaccharide (LPS)-activated murine macrophages	697:749	lipopolysaccharide (LPS)-activated murine macrophages	697:749	The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity.
36232510	7	10	from	fraction	1045:1052	arg1	fractions					1016:1024	Subsequent fractions	1005:1024	Subsequent fractions from the hot water fraction (FRBF and FRBE)	1005:1068	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	4	11	theme	extractions	629:639	arg1	succession					591:600	a succession	589:600	a succession of solvent and solid-phase extractions	589:639	The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions.
36232510	6	12	from	cells	998:1002	arg1	expression					957:966	the pro-inflammatory cytokine expression	927:966	the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells	927:1002	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	13	theme	cytokine	948:955	arg1	expression					957:966	the pro-inflammatory cytokine expression	927:966	the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells	927:1002	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	0	14	theme	Macrophage	121:130	arg1	Model					132:136	a Murine Macrophage Model	112:136	a Murine Macrophage Model	112:136	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	3	15	theme	rice	476:479	arg1	bran					481:484	fermented rice bran	466:484	fermented rice bran that play a key role in its anti-inflammatory function	466:539	This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function.
36232510	2	16	theme	anti-inflammatory	283:299	arg1	effects					301:307	the anti-inflammatory effects	279:307	the anti-inflammatory effects of rice bran	279:320	However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated.
36232510	8	17	theme	bacterial	1194:1202	arg1	metabolite					1204:1213	a bacterial metabolite	1192:1213	a bacterial metabolite of tryptophan	1192:1227	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	8	17	theme	bacterial	1194:1202	arg1	tryptamine					1180:1189	tryptamine	1180:1189	tryptamine	1180:1189	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	0	18	theme	Murine	114:119	arg1	Model					132:136	a Murine Macrophage Model	112:136	a Murine Macrophage Model	112:136	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	6	19	theme	n-hexane	863:870	arg1	fractions					872:880	n-hexane fractions	863:880	n-hexane fractions (FRBC)	863:887	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	19	theme	n-hexane	863:870	arg1	FRBC					883:886	FRBC	883:886	FRBC	883:886	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	9	20	theme	fermented	1374:1382	arg1	bran					1389:1392	fermented rice bran	1374:1392	fermented rice bran	1374:1392	These results indicate that tryptamine may play an important role in the anti-inflammatory effects of fermented rice bran.
36232510	10	21	theme	tryptamine	1450:1459	arg1	effects					1430:1436	the anti-inflammatory effects	1408:1436	the anti-inflammatory effects of FRBE and tryptamine	1408:1459	Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor.
36232510	3	22	theme	bioactive	443:451	arg1	compounds					453:461	bioactive compounds	443:461	bioactive compounds	443:461	This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function.
36232510	7	23	theme	cells	1130:1134	arg1	response					1112:1119	the inflammatory response	1095:1119	the inflammatory response of these cells to LPS	1095:1141	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	0	24	from	Metabolite	24:33	arg1	Bran					53:56	Fermented Rice Bran	38:56	Fermented Rice Bran	38:56	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	6	25	theme	water	805:809	arg1	able					910:913	able	910:913	able	910:913	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	25	theme	water	805:809	arg1	FRBA					822:825	FRBA	822:825	FRBA	822:825	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	25	theme	water	805:809	arg1	fractions					811:819	The hot water fractions	797:819	The hot water fractions (FRBA)	797:826	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	4	26	theme	rice	556:559	arg1	bran					561:564	The fermented rice bran	542:564	The fermented rice bran	542:564	The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions.
36232510	5	27	theme	fermented	646:654	arg1	fractions					666:674	The fermented rice bran fractions	642:674	The fermented rice bran fractions	642:674	The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity.
36232510	6	28	theme	hot	801:803	arg1	able					910:913	able	910:913	able	910:913	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	28	theme	hot	801:803	arg1	FRBA					822:825	FRBA	822:825	FRBA	822:825	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	28	theme	hot	801:803	arg1	fractions					811:819	The hot water fractions	797:819	The hot water fractions (FRBA)	797:826	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	8	29	theme	tryptophan	1218:1227	arg1	metabolite					1204:1213	a bacterial metabolite	1192:1213	a bacterial metabolite of tryptophan	1192:1227	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	8	29	theme	tryptophan	1218:1227	arg1	tryptamine					1180:1189	tryptamine	1180:1189	tryptamine	1180:1189	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	0	30	theme	Microbial	14:22	arg1	Tryptamine					0:9	Tryptamine	0:9	Tryptamine	0:9	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	0	30	theme	Microbial	14:22	arg1	Metabolite					24:33	a Microbial Metabolite	12:33	a Microbial Metabolite in Fermented Rice Bran	12:56	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	4	31	theme	fermented	546:554	arg1	bran					561:564	The fermented rice bran	542:564	The fermented rice bran	542:564	The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions.
36232510	8	32	attach	present	1245:1251	arg1	extracts					1262:1269	these extracts	1256:1269	these extracts	1256:1269	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	8	32	attach	present	1245:1251	arg2	tryptamine					1180:1189	tryptamine	1180:1189	tryptamine	1180:1189	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	8	32	attach	present	1245:1251	arg2	metabolite					1204:1213	a bacterial metabolite	1192:1213	a bacterial metabolite of tryptophan	1192:1227	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	8	33	from	present	1245:1251	arg1	extracts					1262:1269	these extracts	1256:1269	these extracts	1256:1269	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	2	34	theme	bran	317:320	arg1	effects					301:307	the anti-inflammatory effects	279:307	the anti-inflammatory effects of rice bran	279:320	However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated.
36232510	2	34	theme	bran	317:320	arg1	compounds					340:348	the bioactive compounds	326:348	the bioactive compounds that might participate in this function	326:388	However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated.
36232510	3	35	theme	fermented	466:474	arg1	bran					481:484	fermented rice bran	466:484	fermented rice bran that play a key role in its anti-inflammatory function	466:539	This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function.
36232510	8	36	theme	Further	1144:1150	arg1	investigation					1152:1164	Further investigation	1144:1164	Further investigation	1144:1164	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	3	37	theme	anti-inflammatory	514:530	arg1	function					532:539	its anti-inflammatory function	510:539	its anti-inflammatory function	510:539	This study aimed to isolate bioactive compounds in fermented rice bran that play a key role in its anti-inflammatory function.
36232510	2	38	theme	rice	312:315	arg1	bran					317:320	rice bran	312:320	rice bran	312:320	However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated.
36232510	1	39	theme	compounds	221:229	arg1	bioavailability					192:206	bioavailability	192:206	bioavailability	192:206	Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran.
36232510	1	39	theme	compounds	221:229	arg1	composition					176:186	composition	176:186	composition	176:186	Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran.
36232510	7	40	theme	inflammatory	1099:1110	arg1	response					1112:1119	the inflammatory response	1095:1119	the inflammatory response of these cells to LPS	1095:1141	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	7	41	theme	hot	1035:1037	arg1	fraction					1045:1052	the hot water fraction	1031:1052	the hot water fraction (FRBF and FRBE)	1031:1068	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	5	42	theme	-activated	721:730	arg1	macrophages					739:749	lipopolysaccharide (LPS)-activated murine macrophages	697:749	lipopolysaccharide (LPS)-activated murine macrophages	697:749	The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity.
36232510	1	43	theme	rice	234:237	arg1	bran					239:242	rice bran	234:242	rice bran	234:242	Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran.
36232510	0	44	theme	Rice	48:51	arg1	Bran					53:56	Fermented Rice Bran	38:56	Fermented Rice Bran	38:56	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	9	45	theme	important	1323:1331	arg1	role					1333:1336	an important role	1320:1336	an important role	1320:1336	These results indicate that tryptamine may play an important role in the anti-inflammatory effects of fermented rice bran.
36232510	10	46	theme	receptor	1512:1519	arg1	activity					1479:1486	the activity	1475:1486	the activity of the aryl hydrocarbon receptor	1475:1519	Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor.
36232510	1	47	dep	composition	176:186	arg1	the					172:174	the	172:174	the	172:174	Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran.
36232510	7	48	theme	water	1039:1043	arg1	fraction					1045:1052	the hot water fraction	1031:1052	the hot water fraction (FRBF and FRBE)	1031:1068	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	0	49	theme	Fermented	38:46	arg1	Bran					53:56	Fermented Rice Bran	38:56	Fermented Rice Bran	38:56	Tryptamine, a Microbial Metabolite in Fermented Rice Bran Suppressed Lipopolysaccharide-Induced Inflammation in a Murine Macrophage Model.
36232510	8	50	located	present	1245:1251	arg2	tryptamine					1180:1189	tryptamine	1180:1189	tryptamine	1180:1189	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	8	50	located	present	1245:1251	arg1	extracts					1262:1269	these extracts	1256:1269	these extracts	1256:1269	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	8	50	located	present	1245:1251	arg2	metabolite					1204:1213	a bacterial metabolite	1192:1213	a bacterial metabolite of tryptophan	1192:1227	Further investigation revealed that tryptamine, a bacterial metabolite of tryptophan, was abundantly present in these extracts.
36232510	10	51	theme	FRBE	1441:1444	arg1	effects					1430:1436	the anti-inflammatory effects	1408:1436	the anti-inflammatory effects of FRBE and tryptamine	1408:1459	Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor.
36232510	7	52	theme	Subsequent	1005:1014	arg1	fractions					1016:1024	Subsequent fractions	1005:1024	Subsequent fractions from the hot water fraction (FRBF and FRBE)	1005:1068	Subsequent fractions from the hot water fraction (FRBF and FRBE) were also able to reduce the inflammatory response of these cells to LPS.
36232510	6	53	theme	ethanol	833:839	arg1	FRBB					852:855	FRBB	852:855	FRBB	852:855	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	53	theme	ethanol	833:839	arg1	fractions					841:849	50% ethanol fractions	829:849	50% ethanol fractions (FRBB)	829:856	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	4	54	theme	solvent	605:611	arg1	extractions					629:639	solvent and solid-phase extractions	605:639	solvent and solid-phase extractions	605:639	The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions.
36232510	5	55	theme	lipopolysaccharide	697:714	arg1	macrophages					739:749	lipopolysaccharide (LPS)-activated murine macrophages	697:749	lipopolysaccharide (LPS)-activated murine macrophages	697:749	The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity.
36232510	6	56	theme	%	831:831	arg1	FRBB					852:855	FRBB	852:855	FRBB	852:855	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	6	56	theme	%	831:831	arg1	fractions					841:849	50% ethanol fractions	829:849	50% ethanol fractions (FRBB)	829:856	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	9	57	theme	bran	1389:1392	arg1	effects					1363:1369	the anti-inflammatory effects	1341:1369	the anti-inflammatory effects of fermented rice bran	1341:1392	These results indicate that tryptamine may play an important role in the anti-inflammatory effects of fermented rice bran.
36232510	1	58	from	bioavailability	192:206	arg1	bran					239:242	rice bran	234:242	rice bran	234:242	Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran.
36232510	2	59	theme	bioactive	330:338	arg1	compounds					340:348	the bioactive compounds	326:348	the bioactive compounds that might participate in this function	326:388	However, how this process affects the anti-inflammatory effects of rice bran and the bioactive compounds that might participate in this function is yet to be elucidated.
36232510	9	60	theme	anti-inflammatory	1345:1361	arg1	effects					1363:1369	the anti-inflammatory effects	1341:1369	the anti-inflammatory effects of fermented rice bran	1341:1392	These results indicate that tryptamine may play an important role in the anti-inflammatory effects of fermented rice bran.
36232510	6	61	theme	50	829:830	arg1	%					831:831	%	831:831	%	831:831	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	1	62	theme	bioactive	211:219	arg1	compounds					221:229	bioactive compounds	211:229	bioactive compounds	211:229	Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran.
36232510	10	63	theme	aryl	1495:1498	arg1	receptor					1512:1519	the aryl hydrocarbon receptor	1491:1519	the aryl hydrocarbon receptor	1491:1519	Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor.
36232510	4	64	theme	solid-phase	617:627	arg1	extractions					629:639	solvent and solid-phase extractions	605:639	solvent and solid-phase extractions	605:639	The fermented rice bran was fractionated using a succession of solvent and solid-phase extractions.
36232510	5	65	theme	anti-inflammatory	769:785	arg1	activity					787:794	their anti-inflammatory activity	763:794	their anti-inflammatory activity	763:794	The fermented rice bran fractions were then applied to lipopolysaccharide (LPS)-activated murine macrophages to evaluate their anti-inflammatory activity.
36232510	6	66	theme	pro-inflammatory	931:946	arg1	cytokine					948:955	the pro-inflammatory cytokine	927:955	the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells	927:1002	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36232510	1	67	from	composition	176:186	arg1	bran					239:242	rice bran	234:242	rice bran	234:242	Fermentation is thought to alter the composition and bioavailability of bioactive compounds in rice bran.
36232510	10	68	theme	anti-inflammatory	1412:1428	arg1	effects					1430:1436	the anti-inflammatory effects	1408:1436	the anti-inflammatory effects of FRBE and tryptamine	1408:1459	Furthermore, the anti-inflammatory effects of FRBE and tryptamine may depend on the activity of the aryl hydrocarbon receptor.
36232510	6	69	theme	RAW	988:990	arg1	cells					998:1002	LPS-stimulated RAW 264.7 cells	973:1002	LPS-stimulated RAW 264.7 cells	973:1002	The hot water fractions (FRBA), 50% ethanol fractions (FRBB), and n-hexane fractions (FRBC) were all shown to be able to suppress the pro-inflammatory cytokine expression from LPS-stimulated RAW 264.7 cells.
36713178	0	0	theme	submerged	97:105	arg1	mycelium					119:126	Lepista sordida submerged cultivation mycelium	81:126	Lepista sordida submerged cultivation mycelium	81:126	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	1	1	theme	alcohol	326:332	arg1	precipitation					334:346	graded alcohol precipitation	319:346	graded alcohol precipitation	319:346	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	2	2	theme	polysaccharide	355:368	arg1	components					370:379	Three polysaccharide components	349:379	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.	349:546	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	0	3	theme	sordida	89:95	arg1	mycelium					119:126	Lepista sordida submerged cultivation mycelium	81:126	Lepista sordida submerged cultivation mycelium	81:126	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	10	4	from	sordida	1592:1598	arg1	polysaccharides					1563:1577	the bioactive polysaccharides	1549:1577	the bioactive polysaccharides from Lepista sordida	1549:1598	Results are helpful to develop the bioactive polysaccharides from Lepista sordida and beneficial to develop a unique health and functional product in the future.
36713178	4	5	from	nm	791:792	arg1	expression					770:779	the direct expression	759:779	the direct expression at UV 200 nm	759:792	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	5	from	nm	791:792	arg1	bond					714:717	the β-glycosidic bond	697:717	the β-glycosidic bond of three polysaccharide components	697:752	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	5	from	nm	791:792	arg1	peaks					687:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	6	theme	β-glycosidic	701:712	arg1	bond					714:717	the β-glycosidic bond	697:717	the β-glycosidic bond of three polysaccharide components	697:752	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	6	theme	β-glycosidic	701:712	arg1	expression					770:779	the direct expression	759:779	the direct expression at UV 200 nm	759:792	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	6	theme	β-glycosidic	701:712	arg1	peaks					687:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	1	7	theme	Ultrasonic-Micro	248:263	arg1	method					296:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	7	8	theme	polysaccharide	1196:1209	arg1	components					1211:1220	the polysaccharide components	1192:1220	the polysaccharide components	1192:1220	In addition, the polysaccharide components promoted the proliferation of L. casei to some extent.
36713178	4	9	theme	UV	784:785	arg1	nm					791:792	UV 200 nm	784:792	UV 200 nm	784:792	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	1	10	theme	Wave	265:268	arg1	method					296:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	0	11	theme	cultivation	107:117	arg1	mycelium					119:126	Lepista sordida submerged cultivation mycelium	81:126	Lepista sordida submerged cultivation mycelium	81:126	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	8	12	contain	had	1325:1327	arg1	%					1293:1293	80%	1291:1293	80%-LSP-UMSE as the carbon source	1291:1323	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	8	12	contain	had	1325:1327	arg2	production					1341:1350	better acid production	1329:1350	better acid production	1329:1350	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	8	12	contain	had	1325:1327	arg1	%					1279:1279	40%-LSP-UMSE	1277:1288	40%-LSP-UMSE	1277:1288	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	7	13	theme	casei	1255:1259	arg1	proliferation					1235:1247	the proliferation	1231:1247	the proliferation of L. casei to some extent	1231:1274	In addition, the polysaccharide components promoted the proliferation of L. casei to some extent.
36713178	8	14	theme	carbon	1311:1316	arg1	source					1318:1323	the carbon source	1307:1323	the carbon source	1307:1323	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	1	15	theme	Synergy	270:276	arg1	method					296:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	2	16	theme	structural	494:503	arg1	characteristics					505:519	structural characteristics	494:519	structural characteristics	494:519	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	8	17	theme	acid	1336:1339	arg1	production					1341:1350	better acid production	1329:1350	better acid production	1329:1350	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	6	18	theme	better	1086:1091	arg1	activity					1105:1112	better antioxidant activity	1086:1112	better antioxidant activity	1086:1112	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	4	19	theme	characteristic	661:674	arg1	expression					770:779	the direct expression	759:779	the direct expression at UV 200 nm	759:792	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	19	theme	characteristic	661:674	arg1	bond					714:717	the β-glycosidic bond	697:717	the β-glycosidic bond of three polysaccharide components	697:752	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	19	theme	characteristic	661:674	arg1	peaks					687:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	6	20	theme	antioxidant	993:1003	arg1	study					1014:1018	The antioxidant activity study	989:1018	The antioxidant activity study	989:1018	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	8	21	theme	better	1329:1334	arg1	production					1341:1350	better acid production	1329:1350	better acid production	1329:1350	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	2	22	theme	%	416:416	arg1	-LSP-UMSE					417:425	80%-LSP-UMSE	414:425	80%-LSP-UMSE	414:425	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	6	23	theme	antioxidant	1093:1103	arg1	activity					1105:1112	better antioxidant activity	1086:1112	better antioxidant activity	1086:1112	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	9	24	theme	bile	1506:1509	arg1	salts					1511:1515	bile salts	1506:1515	bile salts	1506:1515	Three LSP-UMSE used as a carbon source compared with glucose for culturing L. casei could significantly improve its tolerance to bile salts.
36713178	6	25	theme	"	1169:1169	arg1	method					1171:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	5	26	theme	liquid	950:955	arg1	HPLC					973:976	HPLC	973:976	HPLC	973:976	The three polysaccharide components were mainly composed of glucose, mannose, galactose, and ribose using high-performance liquid chromatography (HPLC) analysis.
36713178	5	26	theme	liquid	950:955	arg1	chromatography					957:970	high-performance liquid chromatography	933:970	high-performance liquid chromatography (HPLC) analysis	933:986	The three polysaccharide components were mainly composed of glucose, mannose, galactose, and ribose using high-performance liquid chromatography (HPLC) analysis.
36713178	2	27	theme	%	384:384	arg1	-LSP-UMSE					385:393	40%-LSP-UMSE	382:393	40%-LSP-UMSE	382:393	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	0	28	theme	Composition	0:10	arg1	analysis					12:19	Composition analysis	0:19	Composition analysis	0:19	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	1	29	theme	Extraction	278:287	arg1	method					296:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	4	30	theme	polysaccharide	728:741	arg1	components					743:752	three polysaccharide components	722:752	three polysaccharide components	722:752	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	5	31	theme	chromatography	957:970	arg1	analysis					979:986	high-performance liquid chromatography (HPLC) analysis	933:986	high-performance liquid chromatography (HPLC) analysis	933:986	The three polysaccharide components were mainly composed of glucose, mannose, galactose, and ribose using high-performance liquid chromatography (HPLC) analysis.
36713178	2	32	theme	40	382:383	arg1	%					384:384	%	384:384	%	384:384	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	2	33	theme	80	414:415	arg1	%					416:416	%	416:416	%	416:416	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	4	34	theme	FT-IR	807:811	arg1	spectroscopy					813:824	FT-IR spectroscopy	807:824	FT-IR spectroscopy	807:824	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	5	35	theme	high-performance	933:948	arg1	HPLC					973:976	HPLC	973:976	HPLC	973:976	The three polysaccharide components were mainly composed of glucose, mannose, galactose, and ribose using high-performance liquid chromatography (HPLC) analysis.
36713178	5	35	theme	high-performance	933:948	arg1	chromatography					957:970	high-performance liquid chromatography	933:970	high-performance liquid chromatography (HPLC) analysis	933:986	The three polysaccharide components were mainly composed of glucose, mannose, galactose, and ribose using high-performance liquid chromatography (HPLC) analysis.
36713178	6	36	theme	HWE	1165:1167	arg1	method					1171:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	5	37	theme	polysaccharide	837:850	arg1	components					852:861	The three polysaccharide components	827:861	The three polysaccharide components	827:861	The three polysaccharide components were mainly composed of glucose, mannose, galactose, and ribose using high-performance liquid chromatography (HPLC) analysis.
36713178	1	38	theme	UMSE	290:293	arg1	method					296:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method	244:301	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	4	39	theme	direct	763:768	arg1	expression					770:779	the direct expression	759:779	the direct expression at UV 200 nm	759:792	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	39	theme	direct	763:768	arg1	bond					714:717	the β-glycosidic bond	697:717	the β-glycosidic bond of three polysaccharide components	697:752	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	39	theme	direct	763:768	arg1	peaks					687:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	9	40	theme	carbon	1402:1407	arg1	source					1409:1414	a carbon source	1400:1414	a carbon source compared with glucose for culturing L. casei	1400:1459	Three LSP-UMSE used as a carbon source compared with glucose for culturing L. casei could significantly improve its tolerance to bile salts.
36713178	6	41	theme	UMSE	1070:1073	arg1	method					1075:1080	the UMSE method	1066:1080	the UMSE method	1066:1080	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	8	42	theme	-LSP-UMSE	1280:1288	arg1	%					1293:1293	80%	1291:1293	80%-LSP-UMSE as the carbon source	1291:1323	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	8	42	theme	-LSP-UMSE	1280:1288	arg1	%					1279:1279	40%-LSP-UMSE	1277:1288	40%-LSP-UMSE	1277:1288	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	2	43	theme	antioxidant	526:536	arg1	activity					538:545	antioxidant activity	526:545	antioxidant activity	526:545	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	4	44	theme	absorption	676:685	arg1	expression					770:779	the direct expression	759:779	the direct expression at UV 200 nm	759:792	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	44	theme	absorption	676:685	arg1	bond					714:717	the β-glycosidic bond	697:717	the β-glycosidic bond of three polysaccharide components	697:752	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	44	theme	absorption	676:685	arg1	peaks					687:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	2	45	theme	%	398:398	arg1	-LSP-UMSE					399:407	60%-LSP-UMSE	396:407	60%-LSP-UMSE	396:407	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	3	46	theme	components	632:641	arg1	casei					602:606	Lactobacillus casei	588:606	Lactobacillus casei of three polysaccharide components	588:641	And the effects on the proliferation of Lactobacillus casei of three polysaccharide components were studied.
36713178	0	47	theme	prebiotics	25:34	arg1	properties					36:45	prebiotics properties	25:45	prebiotics properties	25:45	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	2	48	theme	60	396:397	arg1	%					398:398	%	398:398	%	398:398	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	10	49	theme	bioactive	1553:1561	arg1	polysaccharides					1563:1577	the bioactive polysaccharides	1549:1577	the bioactive polysaccharides from Lepista sordida	1549:1598	Results are helpful to develop the bioactive polysaccharides from Lepista sordida and beneficial to develop a unique health and functional product in the future.
36713178	6	50	theme	traditional	1130:1140	arg1	method					1171:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	6	51	theme	Extraction	1153:1162	arg1	method					1171:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	0	52	theme	polysaccharides	50:64	arg1	analysis					12:19	Composition analysis	0:19	Composition analysis	0:19	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	0	52	theme	polysaccharides	50:64	arg1	properties					36:45	prebiotics properties	25:45	prebiotics properties	25:45	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	6	53	contain	had	1082:1084	arg2	activity					1105:1112	better antioxidant activity	1086:1112	better antioxidant activity	1086:1112	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	6	53	contain	had	1082:1084	arg1	polysaccharides					1038:1052	the polysaccharides	1034:1052	the polysaccharides obtained by the UMSE method	1034:1080	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	6	54	theme	Water	1147:1151	arg1	method					1171:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	3	55	theme	Lactobacillus	588:600	arg1	casei					602:606	Lactobacillus casei	588:606	Lactobacillus casei of three polysaccharide components	588:641	And the effects on the proliferation of Lactobacillus casei of three polysaccharide components were studied.
36713178	4	56	theme	components	743:752	arg1	bond					714:717	the β-glycosidic bond	697:717	the β-glycosidic bond of three polysaccharide components	697:752	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	56	theme	components	743:752	arg1	expression					770:779	the direct expression	759:779	the direct expression at UV 200 nm	759:792	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	4	56	theme	components	743:752	arg1	peaks					687:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks	657:691	The characteristic absorption peaks and the β-glycosidic bond of three polysaccharide components were the direct expression at UV 200 nm using UV and FT-IR spectroscopy.
36713178	8	57	theme	control	1361:1367	arg1	inulin					1369:1374	the control inulin	1357:1374	the control inulin	1357:1374	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	6	58	theme	activity	1005:1012	arg1	study					1014:1018	The antioxidant activity study	989:1018	The antioxidant activity study	989:1018	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	2	59	dep	components	370:379	arg1	obtained					432:439	obtained	432:439	obtained	432:439	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	2	59	dep	components	370:379	arg1	analyzed					453:460	analyzed	453:460	further analyzed the physicochemical properties, structural characteristics, and antioxidant activity	445:545	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	10	60	theme	unique	1628:1633	arg1	health					1635:1640	unique health	1628:1640	unique health	1628:1640	Results are helpful to develop the bioactive polysaccharides from Lepista sordida and beneficial to develop a unique health and functional product in the future.
36713178	3	61	theme	polysaccharide	617:630	arg1	components					632:641	three polysaccharide components	611:641	three polysaccharide components	611:641	And the effects on the proliferation of Lactobacillus casei of three polysaccharide components were studied.
36713178	10	62	from	product	1657:1663	arg1	future					1672:1677	future	1672:1677	future	1672:1677	Results are helpful to develop the bioactive polysaccharides from Lepista sordida and beneficial to develop a unique health and functional product in the future.
36713178	1	63	theme	Lepista	144:150	arg1	polysaccharides					160:174	Lepista sordida polysaccharides	144:174	Lepista sordida polysaccharides (LSP)	144:180	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	1	63	theme	Lepista	144:150	arg1	LSP					177:179	LSP	177:179	LSP	177:179	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	3	64	from	effects	556:562	arg1	proliferation					571:583	the proliferation	567:583	the proliferation of Lactobacillus casei of three polysaccharide components	567:641	And the effects on the proliferation of Lactobacillus casei of three polysaccharide components were studied.
36713178	6	65	theme	Hot	1143:1145	arg1	method					1171:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	the traditional "Hot Water Extraction (HWE)" method	1126:1176	The antioxidant activity study revealed that the polysaccharides obtained by the UMSE method had better antioxidant activity compared to the traditional "Hot Water Extraction (HWE)" method.
36713178	0	66	theme	Lepista	81:87	arg1	mycelium					119:126	Lepista sordida submerged cultivation mycelium	81:126	Lepista sordida submerged cultivation mycelium	81:126	Composition analysis and prebiotics properties of polysaccharides extracted from Lepista sordida submerged cultivation mycelium.
36713178	10	67	theme	functional	1646:1655	arg1	product					1657:1663	functional product	1646:1663	functional product	1646:1663	Results are helpful to develop the bioactive polysaccharides from Lepista sordida and beneficial to develop a unique health and functional product in the future.
36713178	3	68	theme	casei	602:606	arg1	proliferation					571:583	the proliferation	567:583	the proliferation of Lactobacillus casei of three polysaccharide components	567:641	And the effects on the proliferation of Lactobacillus casei of three polysaccharide components were studied.
36713178	1	69	theme	sordida	152:158	arg1	polysaccharides					160:174	Lepista sordida polysaccharides	144:174	Lepista sordida polysaccharides (LSP)	144:180	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	1	69	theme	sordida	152:158	arg1	LSP					177:179	LSP	177:179	LSP	177:179	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	10	70	from	health	1635:1640	arg1	future					1672:1677	future	1672:1677	future	1672:1677	Results are helpful to develop the bioactive polysaccharides from Lepista sordida and beneficial to develop a unique health and functional product in the future.
36713178	2	71	theme	physicochemical	466:480	arg1	properties					482:491	the physicochemical properties	462:491	the physicochemical properties	462:491	Three polysaccharide components: 40%-LSP-UMSE, 60%-LSP-UMSE, and 80%-LSP-UMSE were obtained and further analyzed the physicochemical properties, structural characteristics, and antioxidant activity.
36713178	1	72	theme	graded	319:324	arg1	precipitation					334:346	graded alcohol precipitation	319:346	graded alcohol precipitation	319:346	In this paper, Lepista sordida polysaccharides (LSP) were separated from Lepista sordida (L. sordida) mainly using the Ultrasonic-Micro Wave Synergy Extraction (UMSE) method and purified by graded alcohol precipitation.
36713178	8	73	theme	-LSP-UMSE	1294:1302	arg1	%					1293:1293	80%	1291:1293	80%-LSP-UMSE as the carbon source	1291:1323	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
36713178	8	73	theme	-LSP-UMSE	1294:1302	arg1	%					1279:1279	40%-LSP-UMSE	1277:1288	40%-LSP-UMSE	1277:1288	40%-LSP-UMSE, 80%-LSP-UMSE as the carbon source had better acid production than the control inulin.
35767902	6	0	theme	colon	770:774	arg1	histopathology					776:789	colon histopathology	770:789	colon histopathology	770:789	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	9	1	dep	composition	973:983	arg1	the					969:971	the	969:971	the	969:971	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	4	2	theme	present	466:472	arg1	work					474:477	The present work	462:477	The present work	462:477	The present work determined and compared the effects of different chiral β2AR agonists in acute colitis.
35767902	0	3	theme	β2AR	132:135	arg1	role					124:127	The role	120:127	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.	0:136	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	6	4	theme	Body	704:707	arg1	loss					716:719	Body weight loss	704:719	Body weight loss	704:719	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	3	5	theme	-enantiomers	392:403	arg1	effects					377:383	the effects	373:383	the effects of (RS)-enantiomers of this drug on acute ulcerative colitis	373:444	However, the effects of (RS)-enantiomers of this drug on acute ulcerative colitis remain unknown.
35767902	3	6	theme	ulcerative	427:436	arg1	colitis					438:444	acute ulcerative colitis	421:444	acute ulcerative colitis	421:444	However, the effects of (RS)-enantiomers of this drug on acute ulcerative colitis remain unknown.
35767902	10	7	theme	macrophage	1059:1068	arg1	markers					1070:1076	M1 and M2 macrophage markers	1049:1076	M1 and M2 macrophage markers	1049:1076	The expression of M1 and M2 macrophage markers in the colon were assessed by immunofluorescence staining and Western blotting.
35767902	3	8	from	effects	377:383	arg1	colitis					438:444	acute ulcerative colitis	421:444	acute ulcerative colitis	421:444	However, the effects of (RS)-enantiomers of this drug on acute ulcerative colitis remain unknown.
35767902	2	9	with	drug	340:343	arg1	-isomers					354:361	(RS)-isomers	350:361	(RS)-isomers	350:361	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	11	10	theme	body	1209:1212	arg1	loss					1221:1224	body weight loss	1209:1224	body weight loss	1209:1224	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	12	11	theme	inflammatory	1457:1468	arg1	protein					1470:1476	inflammatory protein	1457:1476	inflammatory protein	1457:1476	(R)-salbutamol downregulated levels of inflammatory protein in LPS-induced human colon tissue specimens.
35767902	4	12	theme	different	518:526	arg1	agonists					540:547	different chiral β2AR agonists	518:547	different chiral β2AR agonists	518:547	The present work determined and compared the effects of different chiral β2AR agonists in acute colitis.
35767902	0	13	theme	agonists	88:95	arg1	-enantiomers					25:36	(R)-enantiomers	22:36	(R)-enantiomers	22:36	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	0	13	theme	agonists	88:95	arg1	R					23:23	R	23:23	R	23:23	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	2	14	theme	β2AR	251:254	arg1	Salbutamol					211:220	Salbutamol	211:220	Salbutamol	211:220	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	2	14	theme	β2AR	251:254	arg1	agonist					257:263	a β2-adrenergic receptor (β2AR) agonist	225:263	a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases	225:318	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	1	15	theme	ulcerative	154:163	arg1	UC					174:175	UC	174:175	UC	174:175	The outcomes of ulcerative colitis (UC) treatment remain unsatisfactory.
35767902	1	15	theme	ulcerative	154:163	arg1	colitis					165:171	ulcerative colitis	154:171	ulcerative colitis (UC) treatment	154:186	The outcomes of ulcerative colitis (UC) treatment remain unsatisfactory.
35767902	4	16	theme	β2AR	535:538	arg1	agonists					540:547	different chiral β2AR agonists	518:547	different chiral β2AR agonists	518:547	The present work determined and compared the effects of different chiral β2AR agonists in acute colitis.
35767902	11	17	theme	colonic	1230:1236	arg1	shortening					1238:1247	colonic shortening	1230:1247	colonic shortening	1230:1247	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	0	18	theme	acute	105:109	arg1	colitis					111:117	acute colitis	105:117	acute colitis	105:117	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	11	19	dep	DAI	1264:1266	arg1	the					1260:1262	the	1260:1262	the	1260:1262	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	5	20	theme	Acute	567:571	arg1	colitis					573:579	Acute colitis	567:579	Acute colitis	567:579	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	14	21	theme	acute	1712:1716	arg1	colitis					1718:1724	acute colitis	1712:1724	acute colitis	1712:1724	The β2AR antagonist ICI-118551 reversed the effect of (R)-salbutamol in ameliorating acute colitis.
35767902	11	22	theme	better	1370:1375	arg1	splenomegaly					1310:1321	splenomegaly	1310:1321	splenomegaly	1310:1321	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	12	23	theme	R	1419:1419	arg1	-salbutamol					1421:1431	(R)-salbutamol	1418:1431	(R)-salbutamol	1418:1431	(R)-salbutamol downregulated levels of inflammatory protein in LPS-induced human colon tissue specimens.
35767902	13	24	theme	gut	1563:1565	arg1	dysbiosis					1567:1575	gut dysbiosis	1563:1575	gut dysbiosis	1563:1575	Furthermore, (R)-salbutamol ameliorated gut dysbiosis and macrophage polarization in mice with colitis.
35767902	9	25	theme	amplicon	923:930	arg1	sequencing					932:941	16S rDNA amplicon sequencing	914:941	16S rDNA amplicon sequencing	914:941	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	0	26	theme	S	47:47	arg1	-enantiomers					49:60	(S)-enantiomers	46:60	(S)-enantiomers	46:60	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	8	27	theme	colitis	896:902	arg1	patients					904:911	colitis patients	896:911	colitis patients	896:911	Colonic biopsies were collected from colitis patients.
35767902	13	28	with	mice	1608:1611	arg1	colitis					1618:1624	colitis	1618:1624	colitis	1618:1624	Furthermore, (R)-salbutamol ameliorated gut dysbiosis and macrophage polarization in mice with colitis.
35767902	10	29	theme	immunofluorescence	1108:1125	arg1	staining					1127:1134	immunofluorescence staining	1108:1134	immunofluorescence staining	1108:1134	The expression of M1 and M2 macrophage markers in the colon were assessed by immunofluorescence staining and Western blotting.
35767902	0	30	theme	-enantiomers	49:60	arg1	effects					11:17	Protective effects	0:17	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.	0:136	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	2	31	theme	receptor	241:248	arg1	Salbutamol					211:220	Salbutamol	211:220	Salbutamol	211:220	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	2	31	theme	receptor	241:248	arg1	agonist					257:263	a β2-adrenergic receptor (β2AR) agonist	225:263	a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases	225:318	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	7	32	theme	cytokine	819:826	arg1	levels					828:833	Inflammatory cytokine levels	806:833	Inflammatory cytokine levels	806:833	Inflammatory cytokine levels were detected by ELISA.
35767902	2	33	with	agonist	257:263	arg1	-isomers					354:361	(RS)-isomers	350:361	(RS)-isomers	350:361	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	13	34	theme	R	1537:1537	arg1	-salbutamol					1539:1549	(R)-salbutamol	1536:1549	(R)-salbutamol	1536:1549	Furthermore, (R)-salbutamol ameliorated gut dysbiosis and macrophage polarization in mice with colitis.
35767902	5	35	theme	sulfate	621:627	arg1	sodium					629:634	3% dextran sulfate sodium	610:634	3% dextran sulfate sodium	610:634	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	0	36	theme	Protective	0:9	arg1	effects					11:17	Protective effects	0:17	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.	0:136	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	12	37	theme	colon	1499:1503	arg1	specimens					1512:1520	LPS-induced human colon tissue specimens	1481:1520	LPS-induced human colon tissue specimens	1481:1520	(R)-salbutamol downregulated levels of inflammatory protein in LPS-induced human colon tissue specimens.
35767902	2	38	theme	RS	351:352	arg1	-isomers					354:361	(RS)-isomers	350:361	(RS)-isomers	350:361	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	3	39	theme	drug	413:416	arg1	-enantiomers					392:403	(RS)-enantiomers	388:403	(RS)-enantiomers of this drug	388:416	However, the effects of (RS)-enantiomers of this drug on acute ulcerative colitis remain unknown.
35767902	6	40	theme	activity	744:751	arg1	DAI					760:762	DAI	760:762	DAI	760:762	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	6	40	theme	activity	744:751	arg1	index					753:757	disease activity index	736:757	disease activity index (DAI)	736:763	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	9	41	theme	relative	989:996	arg1	abundance					998:1006	relative abundance	989:1006	relative abundance	989:1006	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	11	42	theme	inflammatory	1327:1338	arg1	levels					1349:1354	inflammatory cytokine levels	1327:1354	inflammatory cytokine levels	1327:1354	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	2	43	theme	chiral	333:338	arg1	it					325:326	it	325:326	it	325:326	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	2	43	theme	chiral	333:338	arg1	drug					340:343	a chiral drug	331:343	a chiral drug with (RS)-isomers	331:361	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	15	44	theme	new	1820:1822	arg1	mice					1774:1777	the mice	1770:1777	the mice	1770:1777	Taken together, (R)-salbutamol ameliorated the mice with acute colitis, which can serve as a new candidate or lead compound for UC treatment.
35767902	15	44	theme	new	1820:1822	arg1	candidate					1824:1832	a new candidate	1818:1832	a new candidate	1818:1832	Taken together, (R)-salbutamol ameliorated the mice with acute colitis, which can serve as a new candidate or lead compound for UC treatment.
35767902	5	45	theme	different	674:682	arg1	isomers					695:701	orally administered different salbutamol isomers	654:701	orally administered different salbutamol isomers	654:701	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	5	45	theme	different	674:682	arg1	mice					644:647	the mice	640:647	the mice	640:647	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	6	46	theme	colon	722:726	arg1	length					728:733	colon length	722:733	colon length	722:733	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	9	47	theme	gut	1015:1017	arg1	microbiome					1019:1028	the gut microbiome	1011:1028	the gut microbiome	1011:1028	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	10	48	theme	M2	1056:1057	arg1	markers					1070:1076	M1 and M2 macrophage markers	1049:1076	M1 and M2 macrophage markers	1049:1076	The expression of M1 and M2 macrophage markers in the colon were assessed by immunofluorescence staining and Western blotting.
35767902	9	49	theme	rDNA	918:921	arg1	sequencing					932:941	16S rDNA amplicon sequencing	914:941	16S rDNA amplicon sequencing	914:941	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	10	50	theme	M1	1049:1050	arg1	markers					1070:1076	M1 and M2 macrophage markers	1049:1076	M1 and M2 macrophage markers	1049:1076	The expression of M1 and M2 macrophage markers in the colon were assessed by immunofluorescence staining and Western blotting.
35767902	2	51	theme	human	298:302	arg1	diseases					311:318	human airway diseases	298:318	human airway diseases	298:318	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	11	52	theme	histopathological	1272:1288	arg1	scores					1290:1295	histopathological scores	1272:1295	histopathological scores	1272:1295	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	3	53	theme	acute	421:425	arg1	colitis					438:444	acute ulcerative colitis	421:444	acute ulcerative colitis	421:444	However, the effects of (RS)-enantiomers of this drug on acute ulcerative colitis remain unknown.
35767902	10	54	theme	markers	1070:1076	arg1	expression					1035:1044	The expression	1031:1044	The expression of M1 and M2 macrophage markers in the colon	1031:1089	The expression of M1 and M2 macrophage markers in the colon were assessed by immunofluorescence staining and Western blotting.
35767902	8	55	theme	Colonic	859:865	arg1	biopsies					867:874	Colonic biopsies	859:874	Colonic biopsies	859:874	Colonic biopsies were collected from colitis patients.
35767902	11	56	theme	S	1403:1403	arg1	-salbutamol					1405:1415	(S)-salbutamol	1402:1415	(S)-salbutamol	1402:1415	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	2	57	theme	β2-adrenergic	227:239	arg1	Salbutamol					211:220	Salbutamol	211:220	Salbutamol	211:220	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	2	57	theme	β2-adrenergic	227:239	arg1	agonist					257:263	a β2-adrenergic receptor (β2AR) agonist	225:263	a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases	225:318	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	0	58	theme	-enantiomers	25:36	arg1	effects					11:17	Protective effects	0:17	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.	0:136	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	4	59	theme	agonists	540:547	arg1	effects					507:513	the effects	503:513	the effects of different chiral β2AR agonists in acute colitis	503:564	The present work determined and compared the effects of different chiral β2AR agonists in acute colitis.
35767902	10	60	from	expression	1035:1044	arg1	colon					1085:1089	the colon	1081:1089	the colon	1081:1089	The expression of M1 and M2 macrophage markers in the colon were assessed by immunofluorescence staining and Western blotting.
35767902	12	61	theme	protein	1470:1476	arg1	levels					1447:1452	levels	1447:1452	levels of inflammatory protein	1447:1476	(R)-salbutamol downregulated levels of inflammatory protein in LPS-induced human colon tissue specimens.
35767902	4	62	theme	acute	552:556	arg1	colitis					558:564	acute colitis	552:564	acute colitis	552:564	The present work determined and compared the effects of different chiral β2AR agonists in acute colitis.
35767902	12	63	theme	LPS-induced	1481:1491	arg1	specimens					1512:1520	LPS-induced human colon tissue specimens	1481:1520	LPS-induced human colon tissue specimens	1481:1520	(R)-salbutamol downregulated levels of inflammatory protein in LPS-induced human colon tissue specimens.
35767902	1	64	theme	colitis	165:171	arg1	treatment					178:186	ulcerative colitis (UC) treatment	154:186	ulcerative colitis (UC) treatment	154:186	The outcomes of ulcerative colitis (UC) treatment remain unsatisfactory.
35767902	4	65	theme	chiral	528:533	arg1	agonists					540:547	different chiral β2AR agonists	518:547	different chiral β2AR agonists	518:547	The present work determined and compared the effects of different chiral β2AR agonists in acute colitis.
35767902	11	66	theme	weight	1214:1219	arg1	loss					1221:1224	body weight loss	1209:1224	body weight loss	1209:1224	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	15	67	with	mice	1774:1777	arg1	colitis					1790:1796	acute colitis	1784:1796	acute colitis	1784:1796	Taken together, (R)-salbutamol ameliorated the mice with acute colitis, which can serve as a new candidate or lead compound for UC treatment.
35767902	4	68	from	effects	507:513	arg1	colitis					558:564	acute colitis	552:564	acute colitis	552:564	The present work determined and compared the effects of different chiral β2AR agonists in acute colitis.
35767902	9	69	theme	16S	914:916	arg1	sequencing					932:941	16S rDNA amplicon sequencing	914:941	16S rDNA amplicon sequencing	914:941	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	14	70	theme	β2AR	1631:1634	arg1	ICI-118551					1647:1656	The β2AR antagonist ICI-118551	1627:1656	The β2AR antagonist ICI-118551	1627:1656	The β2AR antagonist ICI-118551 reversed the effect of (R)-salbutamol in ameliorating acute colitis.
35767902	10	71	theme	Western	1140:1146	arg1	blotting					1148:1155	Western blotting	1140:1155	Western blotting	1140:1155	The expression of M1 and M2 macrophage markers in the colon were assessed by immunofluorescence staining and Western blotting.
35767902	5	72	theme	%	611:611	arg1	sodium					629:634	3% dextran sulfate sodium	610:634	3% dextran sulfate sodium	610:634	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	14	73	theme	-salbutamol	1684:1694	arg1	effect					1671:1676	the effect	1667:1676	the effect of (R)-salbutamol in ameliorating acute colitis	1667:1724	The β2AR antagonist ICI-118551 reversed the effect of (R)-salbutamol in ameliorating acute colitis.
35767902	11	74	theme	RS	1383:1384	arg1	-salbutamol					1386:1396	(RS)-salbutamol	1382:1396	(RS)-salbutamol	1382:1396	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	5	75	theme	3	610:610	arg1	%					611:611	%	611:611	%	611:611	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	13	76	theme	macrophage	1581:1590	arg1	polarization					1592:1603	macrophage polarization	1581:1603	macrophage polarization	1581:1603	Furthermore, (R)-salbutamol ameliorated gut dysbiosis and macrophage polarization in mice with colitis.
35767902	0	77	theme	receptor	79:86	arg1	agonists					88:95	β2-adrenergic receptor agonists	65:95	β2-adrenergic receptor agonists	65:95	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	11	78	theme	R	1185:1185	arg1	-salbutamol					1187:1197	(R)-salbutamol	1184:1197	(R)-salbutamol	1184:1197	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	5	79	theme	dextran	613:619	arg1	sodium					629:634	3% dextran sulfate sodium	610:634	3% dextran sulfate sodium	610:634	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	5	80	theme	salbutamol	684:693	arg1	isomers					695:701	orally administered different salbutamol isomers	654:701	orally administered different salbutamol isomers	654:701	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	5	80	theme	salbutamol	684:693	arg1	mice					644:647	the mice	640:647	the mice	640:647	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	7	81	theme	Inflammatory	806:817	arg1	levels					828:833	Inflammatory cytokine levels	806:833	Inflammatory cytokine levels	806:833	Inflammatory cytokine levels were detected by ELISA.
35767902	2	82	used	used	284:287	arg2	agonist					257:263	a β2-adrenergic receptor (β2AR) agonist	225:263	a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases	225:318	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	2	82	used	used	284:287	arg2	Salbutamol					211:220	Salbutamol	211:220	Salbutamol	211:220	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	2	82	used	used	284:287	arg2	drug					340:343	a chiral drug	331:343	a chiral drug with (RS)-isomers	331:361	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	2	82	used	used	284:287	arg2	it					325:326	it	325:326	it	325:326	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35767902	15	83	theme	acute	1784:1788	arg1	colitis					1790:1796	acute colitis	1784:1796	acute colitis	1784:1796	Taken together, (R)-salbutamol ameliorated the mice with acute colitis, which can serve as a new candidate or lead compound for UC treatment.
35767902	11	84	theme	cytokine	1340:1347	arg1	levels					1349:1354	inflammatory cytokine levels	1327:1354	inflammatory cytokine levels	1327:1354	The results revealed that (R)-salbutamol prevented body weight loss and colonic shortening, decreased the DAI and histopathological scores, and reduced splenomegaly and inflammatory cytokine levels significantly better than (RS)-salbutamol and (S)-salbutamol.
35767902	12	85	theme	human	1493:1497	arg1	specimens					1512:1520	LPS-induced human colon tissue specimens	1481:1520	LPS-induced human colon tissue specimens	1481:1520	(R)-salbutamol downregulated levels of inflammatory protein in LPS-induced human colon tissue specimens.
35767902	5	86	with	mice	600:603	arg1	sodium					629:634	3% dextran sulfate sodium	610:634	3% dextran sulfate sodium	610:634	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	6	87	theme	disease	736:742	arg1	DAI					760:762	DAI	760:762	DAI	760:762	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	6	87	theme	disease	736:742	arg1	index					753:757	disease activity index	736:757	disease activity index (DAI)	736:763	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	12	88	theme	tissue	1505:1510	arg1	specimens					1512:1520	LPS-induced human colon tissue specimens	1481:1520	LPS-induced human colon tissue specimens	1481:1520	(R)-salbutamol downregulated levels of inflammatory protein in LPS-induced human colon tissue specimens.
35767902	15	89	theme	R	1744:1744	arg1	-salbutamol					1746:1756	(R)-salbutamol	1743:1756	(R)-salbutamol	1743:1756	Taken together, (R)-salbutamol ameliorated the mice with acute colitis, which can serve as a new candidate or lead compound for UC treatment.
35767902	1	90	theme	treatment	178:186	arg1	outcomes					142:149	The outcomes	138:149	The outcomes of ulcerative colitis (UC) treatment	138:186	The outcomes of ulcerative colitis (UC) treatment remain unsatisfactory.
35767902	0	91	theme	β2-adrenergic	65:77	arg1	agonists					88:95	β2-adrenergic receptor agonists	65:95	β2-adrenergic receptor agonists	65:95	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	5	92	theme	administered	661:672	arg1	isomers					695:701	orally administered different salbutamol isomers	654:701	orally administered different salbutamol isomers	654:701	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	5	92	theme	administered	661:672	arg1	mice					644:647	the mice	640:647	the mice	640:647	Acute colitis was established in mice with 3% dextran sulfate sodium and the mice were orally administered different salbutamol isomers.
35767902	3	93	theme	RS	389:390	arg1	-enantiomers					392:403	(RS)-enantiomers	388:403	(RS)-enantiomers of this drug	388:416	However, the effects of (RS)-enantiomers of this drug on acute ulcerative colitis remain unknown.
35767902	14	94	theme	antagonist	1636:1645	arg1	ICI-118551					1647:1656	The β2AR antagonist ICI-118551	1627:1656	The β2AR antagonist ICI-118551	1627:1656	The β2AR antagonist ICI-118551 reversed the effect of (R)-salbutamol in ameliorating acute colitis.
35767902	15	95	theme	lead	1837:1840	arg1	compound					1842:1849	lead compound	1837:1849	lead compound	1837:1849	Taken together, (R)-salbutamol ameliorated the mice with acute colitis, which can serve as a new candidate or lead compound for UC treatment.
35767902	9	96	theme	microbiome	1019:1028	arg1	composition					973:983	composition	973:983	composition	973:983	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	9	96	theme	microbiome	1019:1028	arg1	abundance					998:1006	relative abundance	989:1006	relative abundance	989:1006	16S rDNA amplicon sequencing was carried out to assess the composition and relative abundance of the gut microbiome.
35767902	6	97	theme	weight	709:714	arg1	loss					716:719	Body weight loss	704:719	Body weight loss	704:719	Body weight loss, colon length, disease activity index (DAI), and colon histopathology were assessed.
35767902	0	98	dep	effects	11:17	arg1	role					124:127	The role	120:127	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.	0:136	Protective effects of (R)-enantiomers but not (S)-enantiomers of β2-adrenergic receptor agonists against acute colitis: The role of β2AR.
35767902	2	99	theme	airway	304:309	arg1	diseases					311:318	human airway diseases	298:318	human airway diseases	298:318	Salbutamol is a β2-adrenergic receptor (β2AR) agonist that is frequently used to treat human airway diseases, and it is a chiral drug with (RS)-isomers.
35120661	2	0	theme	strains	314:320	arg1	fitness					269:275	the fitness	265:275	the fitness of various Bacteroidales species and strains in the gut microbiome	265:342	The study by Park and colleagues reveals that the fitness of various Bacteroidales species and strains in the gut microbiome is regulated by butyrate in a glycan-dependent manner.
35120661	1	1	theme	related	149:155	arg1	members					157:163	closely related members	141:163	closely related members	141:163	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	2	2	theme	gut	329:331	arg1	microbiome					333:342	the gut microbiome	325:342	the gut microbiome	325:342	The study by Park and colleagues reveals that the fitness of various Bacteroidales species and strains in the gut microbiome is regulated by butyrate in a glycan-dependent manner.
35120661	1	3	theme	microbial	178:186	arg1	community					188:196	a complex microbial community	168:196	a complex microbial community	168:196	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	2	4	from	fitness	269:275	arg1	microbiome					333:342	the gut microbiome	325:342	the gut microbiome	325:342	The study by Park and colleagues reveals that the fitness of various Bacteroidales species and strains in the gut microbiome is regulated by butyrate in a glycan-dependent manner.
35120661	1	5	theme	members	157:163	arg1	function					129:136	function	129:136	function	129:136	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	1	5	theme	members	157:163	arg1	composition					113:123	composition	113:123	composition	113:123	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	0	6	theme	gut	7:9	arg1	instincts					11:19	gut instincts	7:19	gut instincts	7:19	Beyond gut instincts: Microbe survival depends on sugars and butyrate.
35120661	1	7	dep	factors	75:81	arg1	The					71:73	The	71:73	The	71:73	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	2	8	theme	glycan-dependent	374:389	arg1	manner					391:396	a glycan-dependent manner	372:396	a glycan-dependent manner	372:396	The study by Park and colleagues reveals that the fitness of various Bacteroidales species and strains in the gut microbiome is regulated by butyrate in a glycan-dependent manner.
35120661	0	9	theme	Microbe	22:28	arg1	survival					30:37	Microbe survival	22:37	Microbe survival	22:37	Beyond gut instincts: Microbe survival depends on sugars and butyrate.
35120661	2	10	theme	Bacteroidales	288:300	arg1	species					302:308	various Bacteroidales species	280:308	various Bacteroidales species	280:308	The study by Park and colleagues reveals that the fitness of various Bacteroidales species and strains in the gut microbiome is regulated by butyrate in a glycan-dependent manner.
35120661	1	11	from	composition	113:123	arg1	community					188:196	a complex microbial community	168:196	a complex microbial community	168:196	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	2	12	theme	various	280:286	arg1	species					302:308	various Bacteroidales species	280:308	various Bacteroidales species	280:308	The study by Park and colleagues reveals that the fitness of various Bacteroidales species and strains in the gut microbiome is regulated by butyrate in a glycan-dependent manner.
35120661	1	13	dep	composition	113:123	arg1	the					109:111	the	109:111	the	109:111	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	2	14	theme	species	302:308	arg1	fitness					269:275	the fitness	265:275	the fitness of various Bacteroidales species and strains in the gut microbiome	265:342	The study by Park and colleagues reveals that the fitness of various Bacteroidales species and strains in the gut microbiome is regulated by butyrate in a glycan-dependent manner.
35120661	1	15	theme	complex	170:176	arg1	community					188:196	a complex microbial community	168:196	a complex microbial community	168:196	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
35120661	1	16	from	function	129:136	arg1	community					188:196	a complex microbial community	168:196	a complex microbial community	168:196	The factors and mechanisms that shape the composition and function of closely related members in a complex microbial community are largely unknown.
36708715	6	0	theme	ether	958:962	arg1	extract					964:970	the ether extract	954:970	the ether extract	954:970	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	1	1	theme	Increasing	150:159	arg1	data					161:164	Increasing data	150:164	Increasing data	150:164	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
36708715	4	2	theme	anticancer	594:603	arg1	activities					605:614	anticancer activities	594:614	anticancer activities	594:614	on chemical components and anticancer activities were investigated, as well as the anticancer mechanisms among different extracts.
36708715	0	3	theme	nobile	61:66	arg1	extract					39:45	ether extract	33:45	ether extract of Dendrobium nobile	33:66	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	2	4	with	compositions	389:400	arg1	activity					428:435	excellent anticancer activity	407:435	excellent anticancer activity	407:435	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	2	5	theme	high	352:355	arg1	solvent					363:369	high polar solvent	352:369	high polar solvent	352:369	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	6	6	theme	reticulum	1055:1063	arg1	stress					1065:1070	the endoplasmic reticulum stress	1039:1070	the endoplasmic reticulum stress	1039:1070	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	0	7	theme	protein	92:98	arg1	biosynthesis					100:111	cellular protein biosynthesis	83:111	cellular protein biosynthesis	83:111	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	0	7	theme	protein	92:98	arg1	candidate					22:30	a novel anticancer candidate	3:30	a novel anticancer candidate	3:30	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	8	8	theme	anticancer	1411:1420	arg1	development					1427:1437	anticancer drug development	1411:1437	anticancer drug development	1411:1437	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	6	9	theme	endoplasmic	1043:1053	arg1	reticulum					1055:1063	the endoplasmic reticulum	1039:1063	the endoplasmic reticulum stress	1039:1070	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	0	10	theme	cellular	83:90	arg1	biosynthesis					100:111	cellular protein biosynthesis	83:111	cellular protein biosynthesis	83:111	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	0	10	theme	cellular	83:90	arg1	candidate					22:30	a novel anticancer candidate	3:30	a novel anticancer candidate	3:30	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	2	11	theme	polar	357:361	arg1	solvent					363:369	high polar solvent	352:369	high polar solvent	352:369	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	5	12	theme	anticancer	767:776	arg1	effect					778:783	a stronger anticancer effect	756:783	a stronger anticancer effect	756:783	The results demonstrated that the ether extract exhibited a stronger anticancer effect than ethanol extract, and its anticancer effect was mainly due to weak polar compounds rather than polysaccharides and alkaloids.
36708715	5	13	theme	polar	856:860	arg1	compounds					862:870	weak polar compounds	851:870	weak polar compounds rather than polysaccharides and alkaloids	851:912	The results demonstrated that the ether extract exhibited a stronger anticancer effect than ethanol extract, and its anticancer effect was mainly due to weak polar compounds rather than polysaccharides and alkaloids.
36708715	2	14	theme	anticancer	273:282	arg1	compositions					284:295	most anticancer compositions	268:295	most anticancer compositions from the plant of Dendrobium	268:324	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	6	15	theme	intracellular	1133:1145	arg1	level					1171:1175	the intracellular reactive oxygen species level	1129:1175	the intracellular reactive oxygen species level	1129:1175	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	4	16	theme	different	678:686	arg1	extracts					688:695	different extracts	678:695	different extracts	678:695	on chemical components and anticancer activities were investigated, as well as the anticancer mechanisms among different extracts.
36708715	6	17	theme	Quantitative	915:926	arg1	proteomics					928:937	Quantitative proteomics	915:937	Quantitative proteomics	915:937	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	8	18	theme	drug	1422:1425	arg1	development					1427:1437	anticancer drug development	1411:1437	anticancer drug development	1411:1437	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	2	19	theme	most	268:271	arg1	compositions					284:295	most anticancer compositions	268:295	most anticancer compositions from the plant of Dendrobium	268:324	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	2	20	theme	polar	383:387	arg1	compositions					389:400	weak polar compositions	378:400	weak polar compositions with excellent anticancer activity	378:435	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	2	21	theme	Dendrobium	315:324	arg1	compositions					284:295	most anticancer compositions	268:295	most anticancer compositions from the plant of Dendrobium	268:324	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	8	22	from	research	1456:1463	arg1	drugs					1479:1483	anticancer drugs	1468:1483	anticancer drugs derived from medicinal plants	1468:1513	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	2	23	theme	weak	378:381	arg1	compositions					389:400	weak polar compositions	378:400	weak polar compositions with excellent anticancer activity	378:435	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	1	24	theme	potential	184:192	arg1	properties					205:214	the potential anticancer properties	180:214	the potential anticancer properties of Dendrobium, a traditional Chinese herb	180:256	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
36708715	0	25	theme	anticancer	11:20	arg1	biosynthesis					100:111	cellular protein biosynthesis	83:111	cellular protein biosynthesis	83:111	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	0	25	theme	anticancer	11:20	arg1	candidate					22:30	a novel anticancer candidate	3:30	a novel anticancer candidate	3:30	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	6	26	theme	proteins	1029:1036	arg1	over-expression					1001:1015	the over-expression	997:1015	the over-expression of immature proteins	997:1036	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	0	27	theme	cell	123:126	arg1	stress					128:133	cell stress	123:133	cell stress	123:133	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	8	28	theme	potential	1387:1395	arg1	extract					1361:1367	the ether extract	1351:1367	the ether extract of D. nobile	1351:1380	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	8	28	theme	potential	1387:1395	arg1	candidate					1397:1405	a potential candidate	1385:1405	a potential candidate for anticancer drug development	1385:1437	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	1	29	theme	anticancer	194:203	arg1	properties					205:214	the potential anticancer properties	180:214	the potential anticancer properties of Dendrobium, a traditional Chinese herb	180:256	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
36708715	0	30	theme	novel	5:9	arg1	biosynthesis					100:111	cellular protein biosynthesis	83:111	cellular protein biosynthesis	83:111	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	0	30	theme	novel	5:9	arg1	candidate					22:30	a novel anticancer candidate	3:30	a novel anticancer candidate	3:30	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	6	31	theme	immature	1020:1027	arg1	proteins					1029:1036	immature proteins	1020:1036	immature proteins	1020:1036	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	5	32	theme	ether	732:736	arg1	extract					738:744	the ether extract	728:744	the ether extract	728:744	The results demonstrated that the ether extract exhibited a stronger anticancer effect than ethanol extract, and its anticancer effect was mainly due to weak polar compounds rather than polysaccharides and alkaloids.
36708715	6	33	theme	species	1163:1169	arg1	level					1171:1175	the intracellular reactive oxygen species level	1129:1175	the intracellular reactive oxygen species level	1129:1175	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	2	34	theme	anticancer	417:426	arg1	activity					428:435	excellent anticancer activity	407:435	excellent anticancer activity	407:435	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	8	35	attach	derived	1485:1491	arg2	drugs					1479:1483	anticancer drugs	1468:1483	anticancer drugs derived from medicinal plants	1468:1513	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	8	35	attach	derived	1485:1491	arg1	plants					1508:1513	medicinal plants	1498:1513	medicinal plants	1498:1513	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	4	36	theme	chemical	570:577	arg1	components					579:588	chemical components	570:588	chemical components	570:588	on chemical components and anticancer activities were investigated, as well as the anticancer mechanisms among different extracts.
36708715	8	37	theme	ether	1355:1359	arg1	extract					1361:1367	the ether extract	1351:1367	the ether extract of D. nobile	1351:1380	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	8	37	theme	ether	1355:1359	arg1	candidate					1397:1405	a potential candidate	1385:1405	a potential candidate for anticancer drug development	1385:1437	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	2	38	theme	excellent	407:415	arg1	activity					428:435	excellent anticancer activity	407:435	excellent anticancer activity	407:435	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	6	39	theme	oxygen	1156:1161	arg1	species					1163:1169	reactive oxygen species	1147:1169	the intracellular reactive oxygen species level	1129:1175	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	5	40	theme	stronger	758:765	arg1	effect					778:783	a stronger anticancer effect	756:783	a stronger anticancer effect	756:783	The results demonstrated that the ether extract exhibited a stronger anticancer effect than ethanol extract, and its anticancer effect was mainly due to weak polar compounds rather than polysaccharides and alkaloids.
36708715	6	41	theme	unfolded	1076:1083	arg1	response					1093:1100	unfolded protein response	1076:1100	unfolded protein response	1076:1100	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	1	42	theme	Dendrobium	219:228	arg1	properties					205:214	the potential anticancer properties	180:214	the potential anticancer properties of Dendrobium, a traditional Chinese herb	180:256	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
36708715	5	43	theme	anticancer	815:824	arg1	effect					826:831	its anticancer effect	811:831	its anticancer effect	811:831	The results demonstrated that the ether extract exhibited a stronger anticancer effect than ethanol extract, and its anticancer effect was mainly due to weak polar compounds rather than polysaccharides and alkaloids.
36708715	7	44	theme	cell	1309:1312	arg1	death					1314:1318	cell death	1309:1318	cell death	1309:1318	Eventually, autophagy and apoptosis were activated to cause cell death.
36708715	6	45	theme	oxidative	1205:1213	arg1	stress					1215:1220	oxidative stress	1205:1220	oxidative stress	1205:1220	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	0	46	theme	ether	33:37	arg1	extract					39:45	ether extract	33:45	ether extract of Dendrobium nobile	33:66	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	8	47	theme	polar	1547:1551	arg1	compounds					1553:1561	weak polar compounds	1542:1561	weak polar compounds	1542:1561	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	8	48	theme	anticancer	1468:1477	arg1	drugs					1479:1483	anticancer drugs	1468:1483	anticancer drugs derived from medicinal plants	1468:1513	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	3	49	theme	ether	505:509	arg1	extract					511:517	ether extract	505:517	ether extract	505:517	In this study, the differences between ether extract and ethanol extract of Dendrobium nobile Lindl.
36708715	8	50	theme	nobile	1375:1380	arg1	extract					1361:1367	the ether extract	1351:1367	the ether extract of D. nobile	1351:1380	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	8	50	theme	nobile	1375:1380	arg1	candidate					1397:1405	a potential candidate	1385:1405	a potential candidate for anticancer drug development	1385:1437	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	8	51	theme	future	1449:1454	arg1	research					1456:1463	future research	1449:1463	future research on anticancer drugs derived from medicinal plants	1449:1513	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	3	52	theme	Dendrobium	542:551	arg1	extract					511:517	ether extract	505:517	ether extract	505:517	In this study, the differences between ether extract and ethanol extract of Dendrobium nobile Lindl.
36708715	3	52	theme	Dendrobium	542:551	arg1	extract					531:537	ethanol extract	523:537	ethanol extract	523:537	In this study, the differences between ether extract and ethanol extract of Dendrobium nobile Lindl.
36708715	8	53	theme	D.	1372:1373	arg1	nobile					1375:1380	D. nobile	1372:1380	D. nobile	1372:1380	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	6	54	theme	protein	1085:1091	arg1	response					1093:1100	unfolded protein response	1076:1100	unfolded protein response	1076:1100	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	0	55	theme	Dendrobium	50:59	arg1	nobile					61:66	Dendrobium nobile	50:66	Dendrobium nobile	50:66	As a novel anticancer candidate, ether extract of Dendrobium nobile overstimulates cellular protein biosynthesis to induce cell stress and autophagy.
36708715	4	56	theme	anticancer	650:659	arg1	mechanisms					661:670	the anticancer mechanisms	646:670	the anticancer mechanisms among different extracts	646:695	on chemical components and anticancer activities were investigated, as well as the anticancer mechanisms among different extracts.
36708715	5	57	theme	weak	851:854	arg1	compounds					862:870	weak polar compounds	851:870	weak polar compounds rather than polysaccharides and alkaloids	851:912	The results demonstrated that the ether extract exhibited a stronger anticancer effect than ethanol extract, and its anticancer effect was mainly due to weak polar compounds rather than polysaccharides and alkaloids.
36708715	3	58	theme	ethanol	523:529	arg1	extract					531:537	ethanol extract	523:537	ethanol extract	523:537	In this study, the differences between ether extract and ethanol extract of Dendrobium nobile Lindl.
36708715	6	59	theme	reactive	1147:1154	arg1	species					1163:1169	reactive oxygen species	1147:1169	the intracellular reactive oxygen species level	1129:1175	Quantitative proteomics suggested that the ether extract significantly stimulated the over-expression of immature proteins, the endoplasmic reticulum stress and unfolded protein response were subsequently induced, the intracellular reactive oxygen species level was seriously elevated, and oxidative stress occurred in the meanwhile.
36708715	1	60	theme	traditional	233:243	arg1	herb					253:256	a traditional Chinese herb	231:256	a traditional Chinese herb	231:256	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
36708715	1	60	theme	traditional	233:243	arg1	Dendrobium					219:228	Dendrobium	219:228	Dendrobium	219:228	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
36708715	5	61	theme	ethanol	790:796	arg1	extract					798:804	ethanol extract	790:804	ethanol extract	790:804	The results demonstrated that the ether extract exhibited a stronger anticancer effect than ethanol extract, and its anticancer effect was mainly due to weak polar compounds rather than polysaccharides and alkaloids.
36708715	8	62	theme	medicinal	1498:1506	arg1	plants					1508:1513	medicinal plants	1498:1513	medicinal plants	1498:1513	Our findings demonstrate that the ether extract of D. nobile is a potential candidate for anticancer drug development, and that future research on anticancer drugs derived from medicinal plants should also concentrate on weak polar compounds.
36708715	2	63	from	plant	306:310	arg1	compositions					284:295	most anticancer compositions	268:295	most anticancer compositions from the plant of Dendrobium	268:324	However, most anticancer compositions from the plant of Dendrobium were usually extracted by high polar solvent, while weak polar compositions with excellent anticancer activity remained largely unexplored.
36708715	1	64	theme	Chinese	245:251	arg1	herb					253:256	a traditional Chinese herb	231:256	a traditional Chinese herb	231:256	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
36708715	1	64	theme	Chinese	245:251	arg1	Dendrobium					219:228	Dendrobium	219:228	Dendrobium	219:228	Increasing data has confirmed the potential anticancer properties of Dendrobium, a traditional Chinese herb.
35350328	0	0	theme	Mushroom	87:94	arg1	Compositions					34:45	Metabolic Compositions	24:45	Metabolic Compositions	24:45	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	0	0	theme	Mushroom	87:94	arg1	Elements					57:64	Trace Elements	51:64	Trace Elements	51:64	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	8	1	theme	principal	988:996	arg1	components					998:1007	The principal components	984:1007	The principal components	984:1007	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	8	1	theme	principal	988:996	arg1	different					1041:1049	different	1041:1049	different	1041:1049	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	4	2	theme	molecular	519:527	arg1	basis					529:533	the molecular basis	515:533	the molecular basis of the differentiated usage	515:561	However, there is no holistic insight to elucidate the molecular basis of the differentiated usage.
35350328	0	3	theme	hispidus	78:85	arg1	Mushroom					87:94	Inonotus hispidus Mushroom	69:94	Inonotus hispidus Mushroom	69:94	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	6	4	theme	components	751:760	arg1	kinds					769:773	12 kinds	766:773	12 kinds of trace elements	766:791	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	6	4	theme	components	751:760	arg1	data					724:727	The metabolomic data	708:727	The metabolomic data	708:727	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	6	4	theme	components	751:760	arg1	kinds					732:736	8 kinds	730:736	8 kinds of principal components	730:760	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	8	5	theme	Z.	1204:1205	arg1	samples					1214:1220	Z. jujuba samples	1204:1220	Z. jujuba samples	1204:1220	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	6	6	theme	principal	741:749	arg1	components					751:760	principal components	741:760	principal components	741:760	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	5	7	from	elements	646:653	arg1	hispidus					661:668	I. hispidus	658:668	I. hispidus grown on five different tree species	658:705	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	10	8	theme	molecular	1397:1405	arg1	basis					1407:1411	a molecular basis	1395:1411	a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species	1395:1490	These findings provide a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species.
35350328	0	9	theme	Different	110:118	arg1	Species					125:131	Five Different Tree Species	105:131	Five Different Tree Species	105:131	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	5	10	dep	compositions	623:634	arg1	the					608:610	the	608:610	the	608:610	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	2	11	theme	tree	269:272	arg1	species					274:280	five different tree species	254:280	five different tree species	254:280	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	2	12	dep	grows	245:249	arg1	Morus					283:287	Morus	283:287	Morus	283:287	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	5	13	theme	different	684:692	arg1	species					699:705	five different tree species	679:705	five different tree species	679:705	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	8	14	theme	pumila	1280:1285	arg1	samples					1287:1293	M. pumila samples	1277:1293	M. pumila samples	1277:1293	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	7	15	theme	same	851:854	arg1	metabolites					861:871	the same 1353 metabolites	847:871	the same 1353 metabolites	847:871	The results showed that the same 1353 metabolites were identified in I. hispidus grown on five different tree species, but the relative abundance was different.
35350328	2	16	theme	different	259:267	arg1	species					274:280	five different tree species	254:280	five different tree species	254:280	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	8	17	theme	phenol	1082:1087	arg1	metabolites					1089:1099	phenol metabolites	1082:1099	phenol metabolites	1082:1099	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	8	17	theme	phenol	1082:1087	arg1	example					1056:1062	example	1056:1062	example	1056:1062	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	10	18	theme	unique	1421:1426	arg1	use					1428:1430	the unique use	1417:1430	the unique use of the I. hispidus mushroom grown on different tree species	1417:1490	These findings provide a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species.
35350328	5	19	theme	tree	694:697	arg1	species					699:705	five different tree species	679:705	five different tree species	679:705	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	8	20	theme	trace	1013:1017	arg1	elements					1019:1026	trace elements	1013:1026	trace elements	1013:1026	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	1	21	theme	medicinal	176:184	arg1	mushroom					186:193	a popular edible and medicinal mushroom	155:193	mushroom	186:193	Inonotus hispidus is a popular edible and medicinal mushroom widely used in China.
35350328	0	22	theme	Comparative	0:10	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom	0:94	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	8	23	theme	jujuba	1207:1212	arg1	samples					1214:1220	Z. jujuba samples	1204:1220	Z. jujuba samples	1204:1220	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	10	24	theme	mushroom	1451:1458	arg1	use					1428:1430	the unique use	1417:1430	the unique use of the I. hispidus mushroom grown on different tree species	1417:1490	These findings provide a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species.
35350328	0	25	theme	Metabolic	24:32	arg1	Compositions					34:45	Metabolic Compositions	24:45	Metabolic Compositions	24:45	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	4	26	theme	holistic	485:492	arg1	insight					494:500	no holistic insight	482:500	no holistic insight to elucidate the molecular basis of the differentiated usage	482:561	However, there is no holistic insight to elucidate the molecular basis of the differentiated usage.
35350328	8	27	theme	M.	1277:1278	arg1	samples					1287:1293	M. pumila samples	1277:1293	M. pumila samples	1277:1293	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	6	28	theme	metabolomic	712:722	arg1	kinds					769:773	12 kinds	766:773	12 kinds of trace elements	766:791	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	6	28	theme	metabolomic	712:722	arg1	data					724:727	The metabolomic data	708:727	The metabolomic data	708:727	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	6	28	theme	metabolomic	712:722	arg1	kinds					732:736	8 kinds	730:736	8 kinds of principal components	730:760	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	0	29	theme	Tree	120:123	arg1	Species					125:131	Five Different Tree Species	105:131	Five Different Tree Species	105:131	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	2	30	dep	Hance	315:319	arg1	Mill					390:393	pumila Mill	383:393	pumila Mill	383:393	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	2	31	theme	pumila	383:388	arg1	Mill					390:393	pumila Mill	383:393	pumila Mill	383:393	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	10	32	theme	hispidus	1442:1449	arg1	mushroom					1451:1458	the I. hispidus mushroom	1435:1458	the I. hispidus mushroom grown on different tree species	1435:1490	These findings provide a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species.
35350328	9	33	theme	KEGG	1309:1312	arg1	pathway					1325:1331	the KEGG enrichment pathway	1305:1331	the KEGG enrichment pathway	1305:1331	Further, the KEGG enrichment pathway and metabolic models were established.
35350328	5	34	theme	I.	658:659	arg1	hispidus					661:668	I. hispidus	658:668	I. hispidus grown on five different tree species	658:705	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	8	35	theme	I.	1142:1143	arg1	hispidus					1145:1152	I. hispidus	1142:1152	I. hispidus grown on M. alba	1142:1169	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	0	36	theme	Compositions	34:45	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom	0:94	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	8	37	dep	components	998:1007	arg1	contents					1028:1035	contents	1028:1035	contents	1028:1035	The principal components and trace elements contents are different, for example, polysaccharides, phenol metabolites, Ca, Na, Mg, Fe, and Mn were enriched in I. hispidus grown on M. alba, the flavonoids were enriched in Z. jujuba samples, and the steroids, terpenoids, and Zn were enriched in M. pumila samples.
35350328	6	38	theme	elements	784:791	arg1	kinds					769:773	12 kinds	766:773	12 kinds of trace elements	766:791	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	6	38	theme	elements	784:791	arg1	data					724:727	The metabolomic data	708:727	The metabolomic data	708:727	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	6	38	theme	elements	784:791	arg1	kinds					732:736	8 kinds	730:736	8 kinds of principal components	730:760	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	2	39	theme	hispidus	220:227	arg1	mushroom					229:236	I. hispidus mushroom	217:236	I. hispidus mushroom	217:236	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	7	40	theme	tree	928:931	arg1	species					933:939	five different tree species	913:939	five different tree species	913:939	The results showed that the same 1353 metabolites were identified in I. hispidus grown on five different tree species, but the relative abundance was different.
35350328	3	41	theme	fruiting	408:415	arg1	bodies					417:422	their fruiting bodies	402:422	their fruiting bodies	402:422	, and their fruiting bodies were all separately used in the market.
35350328	0	42	theme	Elements	57:64	arg1	Analysis					12:19	Comparative Analysis	0:19	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom	0:94	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	5	43	theme	metabolite	612:621	arg1	compositions					623:634	metabolite compositions	612:634	metabolite compositions	612:634	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	6	44	theme	trace	778:782	arg1	elements					784:791	trace elements	778:791	trace elements	778:791	The metabolomic data, 8 kinds of principal components and 12 kinds of trace elements, were analyzed in this study.
35350328	2	45	theme	I.	217:218	arg1	mushroom					229:236	I. hispidus mushroom	217:236	I. hispidus mushroom	217:236	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	0	46	theme	Trace	51:55	arg1	Elements					57:64	Trace Elements	51:64	Trace Elements	51:64	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	7	47	theme	relative	950:957	arg1	different					973:981	different	973:981	different	973:981	The results showed that the same 1353 metabolites were identified in I. hispidus grown on five different tree species, but the relative abundance was different.
35350328	7	47	theme	relative	950:957	arg1	abundance					959:967	the relative abundance	946:967	the relative abundance	946:967	The results showed that the same 1353 metabolites were identified in I. hispidus grown on five different tree species, but the relative abundance was different.
35350328	2	48	dep	Morus	283:287	arg1	Hance					315:319	Hance	315:319	Hance	315:319	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	2	48	dep	Morus	283:287	arg1	Mill.					366:370	Mill.	366:370	Mill.	366:370	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	2	48	dep	Morus	283:287	arg1	Ziziphus					350:357	Ziziphus	350:357	Ziziphus	350:357	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	2	48	dep	Morus	283:287	arg1	L.					294:295	Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.	283:394	L.	294:295	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	2	48	dep	Morus	283:287	arg1	Malus					377:381	Malus	377:381	Malus	377:381	I. hispidus mushroom mainly grows on five different tree species (Morus alba L., Ulmus macrocarpa Hance, Fraxinus mandshurica Rupr., Ziziphus jujuba Mill., and Malus pumila Mill.)
35350328	7	49	theme	different	918:926	arg1	species					933:939	five different tree species	913:939	five different tree species	913:939	The results showed that the same 1353 metabolites were identified in I. hispidus grown on five different tree species, but the relative abundance was different.
35350328	9	50	theme	enrichment	1314:1323	arg1	pathway					1325:1331	the KEGG enrichment pathway	1305:1331	the KEGG enrichment pathway	1305:1331	Further, the KEGG enrichment pathway and metabolic models were established.
35350328	4	51	theme	differentiated	542:555	arg1	usage					557:561	the differentiated usage	538:561	the differentiated usage	538:561	However, there is no holistic insight to elucidate the molecular basis of the differentiated usage.
35350328	5	52	theme	trace	640:644	arg1	elements					646:653	trace elements	640:653	trace elements	640:653	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	1	53	theme	Inonotus	134:141	arg1	edible					165:170	edible	165:170	edible	165:170	Inonotus hispidus is a popular edible and medicinal mushroom widely used in China.
35350328	1	53	theme	Inonotus	134:141	arg1	hispidus					143:150	Inonotus hispidus	134:150	Inonotus hispidus	134:150	Inonotus hispidus is a popular edible and medicinal mushroom widely used in China.
35350328	0	54	theme	Inonotus	69:76	arg1	Mushroom					87:94	Inonotus hispidus Mushroom	69:94	Inonotus hispidus Mushroom	69:94	Comparative Analysis of Metabolic Compositions and Trace Elements of Inonotus hispidus Mushroom Grown on Five Different Tree Species.
35350328	10	55	theme	tree	1479:1482	arg1	species					1484:1490	different tree species	1469:1490	different tree species	1469:1490	These findings provide a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species.
35350328	10	56	theme	different	1469:1477	arg1	species					1484:1490	different tree species	1469:1490	different tree species	1469:1490	These findings provide a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species.
35350328	5	57	from	compositions	623:634	arg1	hispidus					661:668	I. hispidus	658:668	I. hispidus grown on five different tree species	658:705	This study aimed to investigate and compare the metabolite compositions and trace elements in I. hispidus grown on five different tree species.
35350328	7	58	theme	I.	892:893	arg1	hispidus					895:902	I. hispidus	892:902	I. hispidus grown on five different tree species	892:939	The results showed that the same 1353 metabolites were identified in I. hispidus grown on five different tree species, but the relative abundance was different.
35350328	9	59	theme	metabolic	1337:1345	arg1	models					1347:1352	metabolic models	1337:1352	metabolic models	1337:1352	Further, the KEGG enrichment pathway and metabolic models were established.
35350328	10	60	theme	I.	1439:1440	arg1	mushroom					1451:1458	the I. hispidus mushroom	1435:1458	the I. hispidus mushroom grown on different tree species	1435:1490	These findings provide a molecular basis for the unique use of the I. hispidus mushroom grown on different tree species.
35350328	4	61	theme	usage	557:561	arg1	basis					529:533	the molecular basis	515:533	the molecular basis of the differentiated usage	515:561	However, there is no holistic insight to elucidate the molecular basis of the differentiated usage.
36563842	0	0	from	colitis	46:52	arg1	mice					57:60	mice	57:60	mice	57:60	Deferasirox alleviates DSS-induced ulcerative colitis in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	6	1	theme	gene	793:796	arg1	sequencing					798:807	16S rRNA gene sequencing	784:807	16S rRNA gene sequencing	784:807	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	13	2	theme	metabolomics	1831:1842	arg1	analysis					1844:1851	Targeted metabolomics analysis	1822:1851	Targeted metabolomics analysis	1822:1851	Targeted metabolomics analysis indicated the SCFAs production enhanced in deferasirox-treated mice.
36563842	5	3	theme	oxidative	621:629	arg1	stress					631:636	oxidative stress	621:636	oxidative stress	621:636	Histological pathologies, inflammatory response, ferrous iron contents, oxidative stress and ferroptosis regulators were determined.
36563842	6	4	theme	16S	784:786	arg1	sequencing					798:807	16S rRNA gene sequencing	784:807	16S rRNA gene sequencing	784:807	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	7	5	theme	FINDINGS	840:847	arg1	Deferasirox					849:859	KEY FINDINGS Deferasirox	836:859	KEY FINDINGS Deferasirox	836:859	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	9	6	theme	TF	1353:1354	arg1	expression					1339:1348	increased expression	1329:1348	increased expression of TF	1329:1354	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	2	7	theme	cell	263:266	arg1	damage					268:273	epithelial cell damage	252:273	epithelial cell damage	252:273	Studies have shown that epithelial cell damage was associated with ferroptosis in UC.
36563842	1	8	theme	bowel	180:184	arg1	disease					186:192	a chronic inflammatory bowel disease	157:192	a chronic inflammatory bowel disease (IBD) caused by multiple factors	157:225	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	8	theme	bowel	180:184	arg1	colitis					141:147	AIMS Ulcerative colitis	125:147	AIMS Ulcerative colitis (UC)	125:152	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	8	theme	bowel	180:184	arg1	IBD					195:197	IBD	195:197	IBD	195:197	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	5	9	theme	ferrous	598:604	arg1	contents					611:618	ferrous iron contents	598:618	ferrous iron contents	598:618	Histological pathologies, inflammatory response, ferrous iron contents, oxidative stress and ferroptosis regulators were determined.
36563842	14	10	theme	DSS-induced	1986:1996	arg1	UC					1998:1999	DSS-induced UC	1986:1999	DSS-induced UC in mice	1986:2007	SIGNIFICANCE Our results suggested that deferasirox could treat DSS-induced UC in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	7	11	theme	index	997:1001	arg1	score					1009:1013	colon length shortening disease activity index (DAI) score	956:1013	colon length shortening disease activity index (DAI) score	956:1013	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	11	theme	index	997:1001	arg1	loss					935:938	weight loss	928:938	weight loss	928:938	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	12	theme	disease	980:986	arg1	DAI					1004:1006	DAI	1004:1006	DAI	1004:1006	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	12	theme	disease	980:986	arg1	index					997:1001	shortening disease activity index	969:1001	colon length shortening disease activity index (DAI) score	956:1013	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	9	13	theme	iron	1203:1206	arg1	accumulation					1208:1219	ferrous iron accumulation	1195:1219	ferrous iron accumulation	1195:1219	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	7	14	theme	length	962:967	arg1	score					1009:1013	colon length shortening disease activity index (DAI) score	956:1013	colon length shortening disease activity index (DAI) score	956:1013	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	14	theme	length	962:967	arg1	loss					935:938	weight loss	928:938	weight loss	928:938	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	14	15	theme	SIGNIFICANCE	1922:1933	arg1	results					1939:1945	SIGNIFICANCE Our results	1922:1945	SIGNIFICANCE Our results	1922:1945	SIGNIFICANCE Our results suggested that deferasirox could treat DSS-induced UC in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	4	16	theme	MAIN	411:414	arg1	METHODS					416:422	MAIN METHODS	411:422	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.	411:547	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	2	17	from	ferroptosis	295:305	arg1	UC					310:311	UC	310:311	UC	310:311	Studies have shown that epithelial cell damage was associated with ferroptosis in UC.
36563842	10	18	from	characteristics	1435:1449	arg1	mice					1466:1469	DSS-induced mice	1454:1469	DSS-induced mice	1454:1469	Deferasirox treatment strongly reversed the alterations caused by ferroptotic characteristics in DSS-induced mice.
36563842	4	19	theme	dextran	458:464	arg1	DSS					487:489	2.5 % dextran sulfate sodium salt (DSS)	452:490	2.5 % dextran sulfate sodium salt (DSS)	452:490	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	3	20	dep	effects	353:359	arg1	the					349:351	the	349:351	the	349:351	Therefore, our research focused on the effects and mechanism of iron chelator deferasirox in UC.
36563842	9	21	theme	ROS	1232:1234	arg1	production					1236:1245	increased ROS production	1222:1245	increased ROS production	1222:1245	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	7	22	theme	survival	941:948	arg1	loss					935:938	weight loss	928:938	weight loss	928:938	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	22	theme	survival	941:948	arg1	rate					950:953	survival rate	941:953	survival rate	941:953	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	1	23	theme	chronic	159:165	arg1	disease					186:192	a chronic inflammatory bowel disease	157:192	a chronic inflammatory bowel disease (IBD) caused by multiple factors	157:225	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	23	theme	chronic	159:165	arg1	colitis					141:147	AIMS Ulcerative colitis	125:147	AIMS Ulcerative colitis (UC)	125:152	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	23	theme	chronic	159:165	arg1	IBD					195:197	IBD	195:197	IBD	195:197	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	9	24	dep	induced	1154:1160	arg1	decreased					1271:1279	decreased	1271:1279	decreased the expression of GPX-4 and FTH	1271:1311	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	4	25	theme	sodium	474:479	arg1	DSS					487:489	2.5 % dextran sulfate sodium salt (DSS)	452:490	2.5 % dextran sulfate sodium salt (DSS)	452:490	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	10	26	theme	Deferasirox	1357:1367	arg1	treatment					1369:1377	Deferasirox treatment	1357:1377	Deferasirox treatment	1357:1377	Deferasirox treatment strongly reversed the alterations caused by ferroptotic characteristics in DSS-induced mice.
36563842	0	27	theme	intestinal	102:111	arg1	microbiota					113:122	intestinal microbiota	102:122	intestinal microbiota	102:122	Deferasirox alleviates DSS-induced ulcerative colitis in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	3	28	from	effects	353:359	arg1	UC					407:408	UC	407:408	UC	407:408	Therefore, our research focused on the effects and mechanism of iron chelator deferasirox in UC.
36563842	14	29	from	UC	1998:1999	arg1	mice					2004:2007	mice	2004:2007	mice	2004:2007	SIGNIFICANCE Our results suggested that deferasirox could treat DSS-induced UC in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	3	30	from	mechanism	365:373	arg1	UC					407:408	UC	407:408	UC	407:408	Therefore, our research focused on the effects and mechanism of iron chelator deferasirox in UC.
36563842	4	31	theme	2.5 	452:455	arg1	DSS					487:489	2.5 % dextran sulfate sodium salt (DSS)	452:490	2.5 % dextran sulfate sodium salt (DSS)	452:490	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	7	32	from	UC	900:901	arg1	mice					906:909	mice	906:909	mice	906:909	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	9	33	with	mice	1165:1168	arg1	UC					1175:1176	UC	1175:1176	UC	1175:1176	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	13	34	theme	deferasirox-treated	1896:1914	arg1	mice					1916:1919	deferasirox-treated mice	1896:1919	deferasirox-treated mice	1896:1919	Targeted metabolomics analysis indicated the SCFAs production enhanced in deferasirox-treated mice.
36563842	0	35	theme	DSS-induced	23:33	arg1	colitis					46:52	DSS-induced ulcerative colitis	23:52	DSS-induced ulcerative colitis in mice	23:60	Deferasirox alleviates DSS-induced ulcerative colitis in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	1	36	theme	AIMS	125:128	arg1	disease					186:192	a chronic inflammatory bowel disease	157:192	a chronic inflammatory bowel disease (IBD) caused by multiple factors	157:225	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	36	theme	AIMS	125:128	arg1	UC					150:151	UC	150:151	UC	150:151	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	36	theme	AIMS	125:128	arg1	colitis					141:147	AIMS Ulcerative colitis	125:147	AIMS Ulcerative colitis (UC)	125:152	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	3	37	theme	deferasirox	392:402	arg1	mechanism					365:373	mechanism	365:373	mechanism	365:373	Therefore, our research focused on the effects and mechanism of iron chelator deferasirox in UC.
36563842	3	37	theme	deferasirox	392:402	arg1	effects					353:359	effects	353:359	effects	353:359	Therefore, our research focused on the effects and mechanism of iron chelator deferasirox in UC.
36563842	10	38	theme	ferroptotic	1423:1433	arg1	characteristics					1435:1449	ferroptotic characteristics	1423:1449	ferroptotic characteristics in DSS-induced mice	1423:1469	Deferasirox treatment strongly reversed the alterations caused by ferroptotic characteristics in DSS-induced mice.
36563842	3	39	theme	iron	378:381	arg1	deferasirox					392:402	iron chelator deferasirox	378:402	iron chelator deferasirox	378:402	Therefore, our research focused on the effects and mechanism of iron chelator deferasirox in UC.
36563842	9	40	theme	GPX-4	1299:1303	arg1	expression					1285:1294	the expression	1281:1294	the expression of GPX-4 and FTH	1281:1311	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	13	41	dep	indicated	1853:1861	arg1	enhanced					1884:1891	enhanced	1884:1891	indicated the SCFAs production enhanced in deferasirox-treated mice	1853:1919	Targeted metabolomics analysis indicated the SCFAs production enhanced in deferasirox-treated mice.
36563842	6	42	theme	fatty	731:735	arg1	acids					737:741	short-chain fatty acids	719:741	short-chain fatty acids (SCFAs) production	719:760	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	6	42	theme	fatty	731:735	arg1	SCFAs					744:748	SCFAs	744:748	SCFAs	744:748	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	9	43	theme	FTH	1309:1311	arg1	expression					1285:1294	the expression	1281:1294	the expression of GPX-4 and FTH	1281:1311	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	8	44	theme	inflammatory	1083:1094	arg1	IL-6					1114:1117	IL-6	1114:1117	IL-6	1114:1117	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	44	theme	inflammatory	1083:1094	arg1	TNF-α					1120:1124	TNF-α	1120:1124	TNF-α	1120:1124	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	44	theme	inflammatory	1083:1094	arg1	INF-γ					1130:1134	INF-γ	1130:1134	INF-γ	1130:1134	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	44	theme	inflammatory	1083:1094	arg1	IL-1β					1107:1111	IL-1β	1107:1111	IL-1β	1107:1111	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	44	theme	inflammatory	1083:1094	arg1	cytokines					1096:1104	pro inflammatory cytokines	1079:1104	pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ)	1079:1135	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	5	45	theme	iron	606:609	arg1	contents					611:618	ferrous iron contents	598:618	ferrous iron contents	598:618	Histological pathologies, inflammatory response, ferrous iron contents, oxidative stress and ferroptosis regulators were determined.
36563842	6	46	theme	microbiota	693:702	arg1	alteration					704:713	Intestinal microbiota alteration	682:713	Intestinal microbiota alteration	682:713	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	7	47	theme	DSS-induced	888:898	arg1	UC					900:901	the DSS-induced UC	884:901	the DSS-induced UC in mice	884:909	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	9	48	theme	increased	1329:1337	arg1	expression					1339:1348	increased expression	1329:1348	increased expression of TF	1329:1354	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	4	49	dep	METHODS	416:422	arg1	induced					441:447	induced	441:447	induced by 2.5 % dextran sulfate sodium salt (DSS)	441:490	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	4	49	dep	METHODS	416:422	arg1	administered					496:507	administered	496:507	administered with deferasirox (10 mg/kg) for 7 days	496:546	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	12	50	theme	deferasirox	1799:1809	arg1	treatment					1811:1819	deferasirox treatment	1799:1819	deferasirox treatment	1799:1819	The results revealed the genera of norank_f__Muribaculaceae, Lachnospiraceae_NK4A136_group, Prevotellaceae_UCG-001, Odoribacter and Blautia were increased distinctly, while Escherichia-Shigella and Streptococcus were significantly decreased by deferasirox treatment.
36563842	13	51	theme	Targeted	1822:1829	arg1	analysis					1844:1851	Targeted metabolomics analysis	1822:1851	Targeted metabolomics analysis	1822:1851	Targeted metabolomics analysis indicated the SCFAs production enhanced in deferasirox-treated mice.
36563842	6	52	theme	rRNA	788:791	arg1	sequencing					798:807	16S rRNA gene sequencing	784:807	16S rRNA gene sequencing	784:807	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	5	53	theme	inflammatory	575:586	arg1	response					588:595	inflammatory response	575:595	inflammatory response	575:595	Histological pathologies, inflammatory response, ferrous iron contents, oxidative stress and ferroptosis regulators were determined.
36563842	11	54	theme	deferasirox	1482:1492	arg1	treatment					1494:1502	deferasirox treatment	1482:1502	deferasirox treatment	1482:1502	Moreover, deferasirox treatment reshaped the composition of intestinal microbiota.
36563842	1	55	theme	inflammatory	167:178	arg1	disease					186:192	a chronic inflammatory bowel disease	157:192	a chronic inflammatory bowel disease (IBD) caused by multiple factors	157:225	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	55	theme	inflammatory	167:178	arg1	colitis					141:147	AIMS Ulcerative colitis	125:147	AIMS Ulcerative colitis (UC)	125:152	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	55	theme	inflammatory	167:178	arg1	IBD					195:197	IBD	195:197	IBD	195:197	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	8	56	dep	cytokines	1096:1104	arg1	TNF-α					1120:1124	TNF-α	1120:1124	TNF-α	1120:1124	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	56	dep	cytokines	1096:1104	arg1	cytokines					1096:1104	pro inflammatory cytokines	1079:1104	pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ)	1079:1135	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	56	dep	cytokines	1096:1104	arg1	IL-6					1114:1117	IL-6	1114:1117	IL-6	1114:1117	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	56	dep	cytokines	1096:1104	arg1	IL-1β					1107:1111	IL-1β	1107:1111	IL-1β	1107:1111	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	56	dep	cytokines	1096:1104	arg1	INF-γ					1130:1134	INF-γ	1130:1134	INF-γ	1130:1134	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	11	57	theme	intestinal	1532:1541	arg1	microbiota					1543:1552	intestinal microbiota	1532:1552	intestinal microbiota	1532:1552	Moreover, deferasirox treatment reshaped the composition of intestinal microbiota.
36563842	7	58	theme	KEY	836:838	arg1	FINDINGS					840:847	KEY FINDINGS	836:847	KEY FINDINGS Deferasirox	836:859	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	11	59	theme	microbiota	1543:1552	arg1	composition					1517:1527	the composition	1513:1527	the composition of intestinal microbiota	1513:1552	Moreover, deferasirox treatment reshaped the composition of intestinal microbiota.
36563842	4	60	theme	UC	428:429	arg1	model					431:435	The UC model	424:435	The UC model	424:435	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	7	61	theme	histology	1019:1027	arg1	score					1029:1033	histology score	1019:1033	histology score	1019:1033	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	61	theme	histology	1019:1027	arg1	loss					935:938	weight loss	928:938	weight loss	928:938	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	1	62	theme	Ulcerative	130:139	arg1	disease					186:192	a chronic inflammatory bowel disease	157:192	a chronic inflammatory bowel disease (IBD) caused by multiple factors	157:225	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	62	theme	Ulcerative	130:139	arg1	UC					150:151	UC	150:151	UC	150:151	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	1	62	theme	Ulcerative	130:139	arg1	colitis					141:147	AIMS Ulcerative colitis	125:147	AIMS Ulcerative colitis (UC)	125:152	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	5	63	theme	ferroptosis	642:652	arg1	regulators					654:663	ferroptosis regulators	642:663	ferroptosis regulators	642:663	Histological pathologies, inflammatory response, ferrous iron contents, oxidative stress and ferroptosis regulators were determined.
36563842	9	64	theme	ferrous	1195:1201	arg1	accumulation					1208:1219	ferrous iron accumulation	1195:1219	ferrous iron accumulation	1195:1219	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	2	65	theme	epithelial	252:261	arg1	damage					268:273	epithelial cell damage	252:273	epithelial cell damage	252:273	Studies have shown that epithelial cell damage was associated with ferroptosis in UC.
36563842	7	66	theme	activity	988:995	arg1	DAI					1004:1006	DAI	1004:1006	DAI	1004:1006	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	66	theme	activity	988:995	arg1	index					997:1001	shortening disease activity index	969:1001	colon length shortening disease activity index (DAI) score	956:1013	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	67	theme	shortening	969:978	arg1	DAI					1004:1006	DAI	1004:1006	DAI	1004:1006	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	67	theme	shortening	969:978	arg1	index					997:1001	shortening disease activity index	969:1001	colon length shortening disease activity index (DAI) score	956:1013	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	4	68	theme	sulfate	466:472	arg1	DSS					487:489	2.5 % dextran sulfate sodium salt (DSS)	452:490	2.5 % dextran sulfate sodium salt (DSS)	452:490	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	1	69	theme	multiple	210:217	arg1	factors					219:225	multiple factors	210:225	multiple factors	210:225	AIMS Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) caused by multiple factors.
36563842	9	70	theme	increased	1222:1230	arg1	production					1236:1245	increased ROS production	1222:1245	increased ROS production	1222:1245	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	7	71	theme	colon	956:960	arg1	score					1009:1013	colon length shortening disease activity index (DAI) score	956:1013	colon length shortening disease activity index (DAI) score	956:1013	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	71	theme	colon	956:960	arg1	loss					935:938	weight loss	928:938	weight loss	928:938	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	4	72	theme	salt	481:484	arg1	DSS					487:489	2.5 % dextran sulfate sodium salt (DSS)	452:490	2.5 % dextran sulfate sodium salt (DSS)	452:490	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	5	73	theme	Histological	549:560	arg1	pathologies					562:572	Histological pathologies	549:572	Histological pathologies	549:572	Histological pathologies, inflammatory response, ferrous iron contents, oxidative stress and ferroptosis regulators were determined.
36563842	6	74	theme	Intestinal	682:691	arg1	alteration					704:713	Intestinal microbiota alteration	682:713	Intestinal microbiota alteration	682:713	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	4	75	theme	%	456:456	arg1	DSS					487:489	2.5 % dextran sulfate sodium salt (DSS)	452:490	2.5 % dextran sulfate sodium salt (DSS)	452:490	MAIN METHODS The UC model was induced by 2.5 % dextran sulfate sodium salt (DSS) and administered with deferasirox (10 mg/kg) for 7 days.
36563842	9	76	theme	GSH	1256:1258	arg1	depletion					1260:1268	GSH depletion	1256:1268	GSH depletion	1256:1268	Ferroptosis was induced in mice with UC, as evidenced by ferrous iron accumulation, increased ROS production, SOD and GSH depletion, decreased the expression of GPX-4 and FTH, accompanied by increased expression of TF.
36563842	8	77	theme	Deferasirox	1036:1046	arg1	treatment					1048:1056	Deferasirox treatment	1036:1056	Deferasirox treatment	1036:1056	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	7	78	theme	weight	928:933	arg1	score					1009:1013	colon length shortening disease activity index (DAI) score	956:1013	colon length shortening disease activity index (DAI) score	956:1013	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	78	theme	weight	928:933	arg1	score					1029:1033	histology score	1019:1033	histology score	1019:1033	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	78	theme	weight	928:933	arg1	loss					935:938	weight loss	928:938	weight loss	928:938	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	7	78	theme	weight	928:933	arg1	rate					950:953	survival rate	941:953	survival rate	941:953	KEY FINDINGS Deferasirox significantly relieved the DSS-induced UC in mice, as evidenced by weight loss, survival rate, colon length shortening disease activity index (DAI) score and histology score.
36563842	0	79	theme	ulcerative	35:44	arg1	colitis					46:52	DSS-induced ulcerative colitis	23:52	DSS-induced ulcerative colitis in mice	23:60	Deferasirox alleviates DSS-induced ulcerative colitis in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	12	80	theme	norank_f__Muribaculaceae	1590:1613	arg1	genera					1580:1585	the genera	1576:1585	the genera of norank_f__Muribaculaceae	1576:1613	The results revealed the genera of norank_f__Muribaculaceae, Lachnospiraceae_NK4A136_group, Prevotellaceae_UCG-001, Odoribacter and Blautia were increased distinctly, while Escherichia-Shigella and Streptococcus were significantly decreased by deferasirox treatment.
36563842	8	81	theme	pro	1079:1081	arg1	IL-6					1114:1117	IL-6	1114:1117	IL-6	1114:1117	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	81	theme	pro	1079:1081	arg1	TNF-α					1120:1124	TNF-α	1120:1124	TNF-α	1120:1124	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	81	theme	pro	1079:1081	arg1	INF-γ					1130:1134	INF-γ	1130:1134	INF-γ	1130:1134	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	81	theme	pro	1079:1081	arg1	IL-1β					1107:1111	IL-1β	1107:1111	IL-1β	1107:1111	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	8	81	theme	pro	1079:1081	arg1	cytokines					1096:1104	pro inflammatory cytokines	1079:1104	pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ)	1079:1135	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	14	82	theme	intestinal	2049:2058	arg1	microbiota					2060:2069	intestinal microbiota	2049:2069	intestinal microbiota	2049:2069	SIGNIFICANCE Our results suggested that deferasirox could treat DSS-induced UC in mice by inhibiting ferroptosis and improving intestinal microbiota.
36563842	3	83	theme	chelator	383:390	arg1	deferasirox					392:402	iron chelator deferasirox	378:402	iron chelator deferasirox	378:402	Therefore, our research focused on the effects and mechanism of iron chelator deferasirox in UC.
36563842	6	84	theme	acids	737:741	arg1	production					751:760	short-chain fatty acids (SCFAs) production	719:760	short-chain fatty acids (SCFAs) production	719:760	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	8	85	theme	cytokines	1096:1104	arg1	level					1070:1074	the level	1066:1074	the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ)	1066:1135	Deferasirox treatment reduced the level of pro inflammatory cytokines (IL-1β, IL-6, TNF-α and INF-γ).
36563842	6	86	theme	targeted	813:820	arg1	metabolomics					822:833	targeted metabolomics	813:833	targeted metabolomics	813:833	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	10	87	theme	DSS-induced	1454:1464	arg1	mice					1466:1469	DSS-induced mice	1454:1469	DSS-induced mice	1454:1469	Deferasirox treatment strongly reversed the alterations caused by ferroptotic characteristics in DSS-induced mice.
36563842	6	88	theme	short-chain	719:729	arg1	acids					737:741	short-chain fatty acids	719:741	short-chain fatty acids (SCFAs) production	719:760	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	6	88	theme	short-chain	719:729	arg1	SCFAs					744:748	SCFAs	744:748	SCFAs	744:748	Intestinal microbiota alteration and short-chain fatty acids (SCFAs) production were analyzed through 16S rRNA gene sequencing and targeted metabolomics.
36563842	13	89	theme	SCFAs	1867:1871	arg1	production					1873:1882	the SCFAs production	1863:1882	the SCFAs production	1863:1882	Targeted metabolomics analysis indicated the SCFAs production enhanced in deferasirox-treated mice.
36639437	8	0	from	efficiency	1517:1526	arg1	trays					1558:1562	degrading postconsumer-PET trays	1531:1562	degrading postconsumer-PET trays	1531:1562	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	8	1	theme	reported	1592:1599	arg1	temperature					1609:1619	the reported optimal temperature	1588:1619	the reported optimal temperature of Fast-PETase	1588:1634	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	8	1	theme	reported	1592:1599	arg1	50 °C					1581:1585	50 °C	1581:1585	50 °C	1581:1585	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	1	2	theme	terephthalate	185:197	arg1	enzymes					217:223	Polyehylene terephthalate (PET) hydrolysing enzymes	173:223	Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost	173:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	1	3	from	biodegradation	257:270	arg1	industry					282:289	industry	282:289	industry	282:289	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	1	4	theme	PET	275:277	arg1	biodegradation					257:270	the biodegradation	253:270	the biodegradation of PET in industry	253:289	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	7	5	theme	same	1432:1435	arg1	result					1437:1442	the same result	1428:1442	the same result	1428:1442	This platform was also applied for the preparation of a famous variant of IsPETase, Fast-PETase, and the same result was achieved.
36639437	9	6	theme	PET	1775:1777	arg1	biodegradation					1779:1792	PET biodegradation	1775:1792	PET biodegradation	1775:1792	The present study provides a strategy to modulate thermostable IsPETase through glycosylation engineering and paves the way for promoting PET biodegradation from laboratories to factories.
36639437	2	7	theme	Pichia	374:379	arg1	pastoris					381:388	Pichia pastoris	374:388	Pichia pastoris	374:388	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	0	8	theme	post-translational	102:119	arg1	modification					128:139	its post-translational glycan modification	98:139	its post-translational glycan modification	98:139	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	4	9	theme	specific	839:846	arg1	activity					848:855	the specific activity	835:855	the specific activity of IsPETase	835:867	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	4	10	theme	Endo-β-N-acetylglucosaminidase	953:982	arg1	H					984:984	Endo-β-N-acetylglucosaminidase H	953:984	Endo-β-N-acetylglucosaminidase H (Endo H)	953:993	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	4	10	theme	Endo-β-N-acetylglucosaminidase	953:982	arg1	H					992:992	Endo H	987:992	Endo H	987:992	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	5	11	theme	oligo-saccharide	1120:1135	arg1	residues					1137:1144	the remaining mono- and oligo-saccharide residues	1096:1144	the remaining mono- and oligo-saccharide residues on the protein molecules	1096:1169	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	6	12	theme	PET	1289:1291	arg1	flakes					1293:1298	untreated PET flakes	1279:1298	untreated PET flakes	1279:1298	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	3	13	gly	glycosylated	681:692	arg1	IsPETase					661:668	IsPETase	661:668	IsPETase	661:668	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	2	14	theme	present	299:305	arg1	study					307:311	the present study	295:311	the present study	295:311	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	2	15	theme	pastoris	381:388	arg1	glycosylation					357:369	the post-translational glycosylation	334:369	the post-translational glycosylation of Pichia pastoris	334:388	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	3	16	with	expression	711:720	arg1	pastoris					730:737	P. pastoris	727:737	P. pastoris	727:737	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	2	17	from	201-F6	485:490	arg1	expression					438:447	the heterologous expression	421:447	the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase)	421:501	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	4	18	theme	N-linked	903:910	arg1	chains					927:932	the bulky N-linked polysaccharide chains	893:932	the bulky N-linked polysaccharide chains	893:932	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	6	19	theme	deglycosylated	1200:1213	arg1	IsPETase					1215:1222	the partially deglycosylated IsPETase	1186:1222	the partially deglycosylated IsPETase	1186:1222	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	6	19	theme	deglycosylated	1200:1213	arg1	able					1228:1231	able	1228:1231	able	1228:1231	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	3	20	theme	O-linked	544:551	arg1	glycosylation					553:565	O-linked glycosylation	544:565	O-linked glycosylation	544:565	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	2	21	theme	remarkable	401:410	arg1	host					412:415	a remarkable host	399:415	a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase)	399:501	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	1	22	theme	PET	200:202	arg1	enzymes					217:223	Polyehylene terephthalate (PET) hydrolysing enzymes	173:223	Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost	173:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	0	23	theme	glycan	121:126	arg1	modification					128:139	its post-translational glycan modification	98:139	its post-translational glycan modification	98:139	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	3	24	theme	specific	759:766	arg1	activity					768:775	specific activity	759:775	specific activity	759:775	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	4	25	theme	bulky	897:901	arg1	chains					927:932	the bulky N-linked polysaccharide chains	893:932	the bulky N-linked polysaccharide chains	893:932	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	7	26	theme	variant	1390:1396	arg1	preparation					1366:1376	the preparation	1362:1376	the preparation of a famous variant of IsPETase, Fast-PETase,	1362:1422	This platform was also applied for the preparation of a famous variant of IsPETase, Fast-PETase, and the same result was achieved.
36639437	3	27	theme	glycosylation	553:565	arg1	advantage					511:519	advantage	511:519	advantage of the abundant N- and O-linked glycosylation sites in IsPETase	511:583	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	27	theme	glycosylation	553:565	arg1	modification					622:633	the efficient post-translational modification	589:633	the efficient post-translational modification in endoplasmic reticulum	589:658	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	8	28	theme	deglycosylated	1468:1481	arg1	Fast-PETase					1483:1493	Partially deglycosylated Fast-PETase	1458:1493	Partially deglycosylated Fast-PETase	1458:1493	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	2	29	theme	sakaiensis	474:483	arg1	IsPETase					493:500	IsPETase	493:500	IsPETase	493:500	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	2	29	theme	sakaiensis	474:483	arg1	201-F6					485:490	Ideonella sakaiensis 201-F6	464:490	Ideonella sakaiensis 201-F6 (IsPETase)	464:501	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	8	30	theme	elevated	1508:1515	arg1	efficiency					1517:1526	elevated efficiency	1508:1526	elevated efficiency in degrading postconsumer-PET trays	1508:1562	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	1	31	from	preparation	158:168	arg1	low-cost					228:235	low-cost	228:235	low-cost	228:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	6	32	theme	depolymerized	1260:1272	arg1	raw					1274:1276	depolymerized raw	1260:1276	depolymerized raw	1260:1276	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	8	33	theme	Fast-PETase	1624:1634	arg1	temperature					1609:1619	the reported optimal temperature	1588:1619	the reported optimal temperature of Fast-PETase	1588:1634	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	8	33	theme	Fast-PETase	1624:1634	arg1	50 °C					1581:1585	50 °C	1581:1585	50 °C	1581:1585	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	1	34	theme	hydrolysing	205:215	arg1	enzymes					217:223	Polyehylene terephthalate (PET) hydrolysing enzymes	173:223	Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost	173:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	2	35	theme	Ideonella	464:472	arg1	IsPETase					493:500	IsPETase	493:500	IsPETase	493:500	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	2	35	theme	Ideonella	464:472	arg1	201-F6					485:490	Ideonella sakaiensis 201-F6	464:490	Ideonella sakaiensis 201-F6 (IsPETase)	464:501	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	3	36	theme	enzyme	804:809	arg1	activity					768:775	specific activity	759:775	specific activity	759:775	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	36	theme	enzyme	804:809	arg1	thermostability					781:795	thermostability	781:795	thermostability	781:795	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	4	37	theme	polysaccharide	912:925	arg1	chains					927:932	the bulky N-linked polysaccharide chains	893:932	the bulky N-linked polysaccharide chains	893:932	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	3	38	from	advantage	511:519	arg1	IsPETase					576:583	IsPETase	576:583	IsPETase	576:583	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	38	from	advantage	511:519	arg1	reticulum					650:658	endoplasmic reticulum	638:658	endoplasmic reticulum	638:658	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	39	theme	efficient	593:601	arg1	modification					622:633	the efficient post-translational modification	589:633	the efficient post-translational modification in endoplasmic reticulum	589:658	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	1	40	theme	enzymes	217:223	arg1	preparation					158:168	The large-scale preparation	142:168	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost	142:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	1	40	theme	enzymes	217:223	arg1	critical					240:247	critical	240:247	critical	240:247	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	8	41	theme	degrading	1531:1539	arg1	trays					1558:1562	degrading postconsumer-PET trays	1531:1562	degrading postconsumer-PET trays	1531:1562	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	3	42	dep	N-	537:538	arg1	sites					567:571	sites	567:571	sites	567:571	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	42	dep	N-	537:538	arg1	the					524:526	the	524:526	the	524:526	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	43	from	modification	622:633	arg1	IsPETase					576:583	IsPETase	576:583	IsPETase	576:583	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	43	from	modification	622:633	arg1	reticulum					650:658	endoplasmic reticulum	638:658	endoplasmic reticulum	638:658	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	7	44	theme	famous	1383:1388	arg1	variant					1390:1396	a famous variant	1381:1396	a famous variant of IsPETase	1381:1408	This platform was also applied for the preparation of a famous variant of IsPETase, Fast-PETase, and the same result was achieved.
36639437	7	44	theme	famous	1383:1388	arg1	Fast-PETase					1411:1421	Fast-PETase	1411:1421	Fast-PETase	1411:1421	This platform was also applied for the preparation of a famous variant of IsPETase, Fast-PETase, and the same result was achieved.
36639437	2	45	theme	PETase	452:457	arg1	expression					438:447	the heterologous expression	421:447	the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase)	421:501	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	5	46	theme	high	1064:1067	arg1	thermostability					1069:1083	high thermostability	1064:1083	high thermostability	1064:1083	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	3	47	theme	abundant	528:535	arg1	N-					537:538	abundant N-	528:538	abundant N-	528:538	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	6	48	gly	deglycosylated	1200:1213	arg1	IsPETase					1215:1222	the partially deglycosylated IsPETase	1186:1222	the partially deglycosylated IsPETase	1186:1222	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	6	48	gly	deglycosylated	1200:1213	arg1	able					1228:1231	able	1228:1231	able	1228:1231	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	6	49	dep	3	1319:1319	arg1	to					1316:1317	to	1316:1317	to	1316:1317	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	9	50	theme	glycosylation	1717:1729	arg1	engineering					1731:1741	glycosylation engineering	1717:1741	glycosylation engineering	1717:1741	The present study provides a strategy to modulate thermostable IsPETase through glycosylation engineering and paves the way for promoting PET biodegradation from laboratories to factories.
36639437	9	51	theme	thermostable	1687:1698	arg1	IsPETase					1700:1707	thermostable IsPETase	1687:1707	thermostable IsPETase	1687:1707	The present study provides a strategy to modulate thermostable IsPETase through glycosylation engineering and paves the way for promoting PET biodegradation from laboratories to factories.
36639437	3	52	theme	secretory	701:709	arg1	expression					711:720	secretory expression	701:720	secretory expression	701:720	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	5	53	theme	mono-	1110:1114	arg1	residues					1137:1144	the remaining mono- and oligo-saccharide residues	1096:1144	the remaining mono- and oligo-saccharide residues on the protein molecules	1096:1169	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	7	54	theme	IsPETase	1401:1408	arg1	variant					1390:1396	a famous variant	1381:1396	a famous variant of IsPETase	1381:1408	This platform was also applied for the preparation of a famous variant of IsPETase, Fast-PETase, and the same result was achieved.
36639437	7	54	theme	IsPETase	1401:1408	arg1	Fast-PETase					1411:1421	Fast-PETase	1411:1421	Fast-PETase	1411:1421	This platform was also applied for the preparation of a famous variant of IsPETase, Fast-PETase, and the same result was achieved.
36639437	2	55	theme	heterologous	425:436	arg1	expression					438:447	the heterologous expression	421:447	the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase)	421:501	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	0	56	from	sakaiensis	68:77	arg1	activity					14:21	activity	14:21	activity	14:21	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	0	56	from	sakaiensis	68:77	arg1	thermostability					27:41	thermostability	27:41	thermostability	27:41	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	3	57	theme	endoplasmic	638:648	arg1	reticulum					650:658	endoplasmic reticulum	638:658	endoplasmic reticulum	638:658	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	5	58	gly	deglycosylated	1023:1036	arg1	IsPETase					1038:1045	the partially deglycosylated IsPETase	1009:1045	the partially deglycosylated IsPETase	1009:1045	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	4	59	link	N-linked	903:910	arg1	chains					927:932	the bulky N-linked polysaccharide chains	893:932	the bulky N-linked polysaccharide chains	893:932	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	3	60	link	O-linked	544:551	arg1	glycosylation					553:565	O-linked glycosylation	544:565	O-linked glycosylation	544:565	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	2	61	gly	glycosylation	357:369	arg1	pastoris					381:388	Pichia pastoris	374:388	Pichia pastoris	374:388	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	3	62	theme	post-translational	603:620	arg1	modification					622:633	the efficient post-translational modification	589:633	the efficient post-translational modification in endoplasmic reticulum	589:658	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	9	63	theme	present	1641:1647	arg1	study					1649:1653	The present study	1637:1653	The present study	1637:1653	The present study provides a strategy to modulate thermostable IsPETase through glycosylation engineering and paves the way for promoting PET biodegradation from laboratories to factories.
36639437	0	64	theme	PETase	46:51	arg1	activity					14:21	activity	14:21	activity	14:21	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	0	64	theme	PETase	46:51	arg1	thermostability					27:41	thermostability	27:41	thermostability	27:41	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	6	65	theme	untreated	1279:1287	arg1	flakes					1293:1298	untreated PET flakes	1279:1298	untreated PET flakes	1279:1298	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	8	66	gly	deglycosylated	1468:1481	arg1	Fast-PETase					1483:1493	Partially deglycosylated Fast-PETase	1458:1493	Partially deglycosylated Fast-PETase	1458:1493	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	6	67	dep	flakes	1293:1298	arg1	days					1321:1324	2 to 3 days	1314:1324	2 to 3 days	1314:1324	Consequently, the partially deglycosylated IsPETase was able to be applied at 50 °C and depolymerized raw, untreated PET flakes completely in 2 to 3 days.
36639437	8	68	theme	postconsumer-PET	1541:1556	arg1	trays					1558:1562	degrading postconsumer-PET trays	1531:1562	degrading postconsumer-PET trays	1531:1562	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	1	69	from	low-cost	228:235	arg1	preparation					158:168	The large-scale preparation	142:168	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost	142:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	1	69	from	low-cost	228:235	arg1	critical					240:247	critical	240:247	critical	240:247	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	1	70	theme	large-scale	146:156	arg1	preparation					158:168	The large-scale preparation	142:168	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost	142:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	1	70	theme	large-scale	146:156	arg1	critical					240:247	critical	240:247	critical	240:247	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	5	71	theme	deglycosylated	1023:1036	arg1	IsPETase					1038:1045	the partially deglycosylated IsPETase	1009:1045	the partially deglycosylated IsPETase	1009:1045	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	4	72	theme	Endo	987:990	arg1	H					984:984	Endo-β-N-acetylglucosaminidase H	953:984	Endo-β-N-acetylglucosaminidase H (Endo H)	953:993	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	4	72	theme	Endo	987:990	arg1	H					992:992	Endo H	987:992	Endo H	987:992	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	5	73	theme	protein	1153:1159	arg1	molecules					1161:1169	the protein molecules	1149:1169	the protein molecules	1149:1169	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	0	74	dep	activity	14:21	arg1	the					10:12	the	10:12	the	10:12	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	5	75	from	residues	1137:1144	arg1	molecules					1161:1169	the protein molecules	1149:1169	the protein molecules	1149:1169	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	2	76	theme	post-translational	338:355	arg1	glycosylation					357:369	the post-translational glycosylation	334:369	the post-translational glycosylation of Pichia pastoris	334:388	In the present study, we demonstrate that the post-translational glycosylation of Pichia pastoris makes it a remarkable host for the heterologous expression of PETase from Ideonella sakaiensis 201-F6 (IsPETase).
36639437	4	77	theme	IsPETase	860:867	arg1	activity					848:855	the specific activity	835:855	the specific activity of IsPETase	835:867	Moreover, the specific activity of IsPETase increased further after the bulky N-linked polysaccharide chains were eliminated by Endo-β-N-acetylglucosaminidase H (Endo H).
36639437	1	78	from	enzymes	217:223	arg1	low-cost					228:235	low-cost	228:235	low-cost	228:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	3	79	theme	N-	537:538	arg1	advantage					511:519	advantage	511:519	advantage of the abundant N- and O-linked glycosylation sites in IsPETase	511:583	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	3	79	theme	N-	537:538	arg1	modification					622:633	the efficient post-translational modification	589:633	the efficient post-translational modification in endoplasmic reticulum	589:658	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
36639437	0	80	theme	Ideonella	58:66	arg1	sakaiensis					68:77	Ideonella sakaiensis	58:77	Ideonella sakaiensis	58:77	Improving the activity and thermostability of PETase from Ideonella sakaiensis through modulating its post-translational glycan modification.
36639437	8	81	theme	optimal	1601:1607	arg1	temperature					1609:1619	the reported optimal temperature	1588:1619	the reported optimal temperature of Fast-PETase	1588:1634	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	8	81	theme	optimal	1601:1607	arg1	50 °C					1581:1585	50 °C	1581:1585	50 °C	1581:1585	Partially deglycosylated Fast-PETase demonstrates elevated efficiency in degrading postconsumer-PET trays under 55 °C than 50 °C, the reported optimal temperature of Fast-PETase.
36639437	5	82	theme	remaining	1100:1108	arg1	residues					1137:1144	the remaining mono- and oligo-saccharide residues	1096:1144	the remaining mono- and oligo-saccharide residues on the protein molecules	1096:1169	Importantly, the partially deglycosylated IsPETase still maintained high thermostability because of the remaining mono- and oligo-saccharide residues on the protein molecules.
36639437	1	83	theme	Polyehylene	173:183	arg1	enzymes					217:223	Polyehylene terephthalate (PET) hydrolysing enzymes	173:223	Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost	173:235	The large-scale preparation of Polyehylene terephthalate (PET) hydrolysing enzymes in low-cost is critical for the biodegradation of PET in industry.
36639437	3	84	dep	activity	768:775	arg1	the					755:757	the	755:757	the	755:757	Taking advantage of the abundant N- and O-linked glycosylation sites in IsPETase and the efficient post-translational modification in endoplasmic reticulum, IsPETase is heavily glycosylated during secretory expression with P. pastoris, which improves the specific activity and thermostability of the enzyme dramatically.
34981577	9	0	theme	N-glycans	1871:1879	arg1	biosynthesis					1830:1841	the biosynthesis	1826:1841	the biosynthesis of tri- and tetra-antennary N-glycans	1826:1879	Taken together, these data are the first to demonstrate that O-GlcNAcylation specifically governs the biosynthesis of tri- and tetra-antennary N-glycans via the OGT-SLC35A3-GnT-IV axis.
34981577	9	1	theme	tri-	1846:1849	arg1	N-glycans					1871:1879	tri- and tetra-antennary N-glycans	1846:1879	tri- and tetra-antennary N-glycans	1846:1879	Taken together, these data are the first to demonstrate that O-GlcNAcylation specifically governs the biosynthesis of tri- and tetra-antennary N-glycans via the OGT-SLC35A3-GnT-IV axis.
34981577	4	2	theme	GlcNAc-branched	701:715	arg1	N-glycans					731:739	GlcNAc-branched tri-antennary N-glycans	701:739	GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV)	701:796	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	3	theme	lectin	608:613	arg1	blotting					615:622	lectin blotting	608:622	lectin blotting	608:622	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	1	4	link	O-linked	129:136	arg1	modifications					210:222	important protein post-translational modifications	173:222	important protein post-translational modifications that are orchestrated by a diverse set of gene products	173:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	4	link	O-linked	129:136	arg1	O-GlcNAc					159:166	O-GlcNAc	159:166	O-GlcNAc	159:166	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	4	link	O-linked	129:136	arg1	glycosylation					111:123	N-Linked glycosylation	102:123	N-Linked glycosylation	102:123	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	4	link	O-linked	129:136	arg1	N-acetylglucosamine					138:156	O-linked N-acetylglucosamine	129:156	O-linked N-acetylglucosamine (O-GlcNAc)	129:167	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	8	5	theme	cell	1590:1593	arg1	spreading					1595:1603	cell spreading	1590:1603	cell spreading	1590:1603	Furthermore, we found that SLC35A3-KO enhances cell spreading and suppresses both cell migration and cell proliferation, which is similar to the phenomena observed in the OGT-KD cells.
34981577	8	6	located	observed	1698:1705	arg2	phenomena					1688:1696	the phenomena	1684:1696	the phenomena observed in the OGT-KD cells	1684:1725	Furthermore, we found that SLC35A3-KO enhances cell spreading and suppresses both cell migration and cell proliferation, which is similar to the phenomena observed in the OGT-KD cells.
34981577	8	6	located	observed	1698:1705	arg1	cells					1721:1725	the OGT-KD cells	1710:1725	the OGT-KD cells	1710:1725	Furthermore, we found that SLC35A3-KO enhances cell spreading and suppresses both cell migration and cell proliferation, which is similar to the phenomena observed in the OGT-KD cells.
34981577	4	7	theme	wild	929:932	arg1	cells					939:943	wild type cells	929:943	wild type cells	929:943	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	7	8	theme	N-acetylglucosaminyltransferase	1365:1395	arg1	blot					1408:1411	N-acetylglucosaminyltransferase V. Western blot	1365:1411	N-acetylglucosaminyltransferase V. Western blot	1365:1411	Furthermore, co-immunoprecipitation experiments have shown that SLC35A3 interacts with GnT-IV, but not with N-acetylglucosaminyltransferase V. Western blot and chemoenzymatic labeling assay have confirmed that OGT modifies SLC35A3 and that O-GlcNAcylation contributes to its stability.
34981577	8	9	theme	OGT-KD	1714:1719	arg1	cells					1721:1725	the OGT-KD cells	1710:1725	the OGT-KD cells	1710:1725	Furthermore, we found that SLC35A3-KO enhances cell spreading and suppresses both cell migration and cell proliferation, which is similar to the phenomena observed in the OGT-KD cells.
34981577	6	10	theme	tri-	1221:1224	arg1	N-glycans					1246:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	5	11	from	transporter	1036:1046	arg1	mammals					1051:1057	mammals	1051:1057	mammals	1051:1057	We examined this specific alteration by focusing on SLC35A3, which is the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation.
34981577	6	12	theme	SLC35A3 specifically	1146:1165	arg1	deficiency					1132:1141	a deficiency	1130:1141	a deficiency of SLC35A3 specifically	1130:1165	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	8	13	theme	cell	1625:1628	arg1	migration					1630:1638	cell migration	1625:1638	cell migration	1625:1638	Furthermore, we found that SLC35A3-KO enhances cell spreading and suppresses both cell migration and cell proliferation, which is similar to the phenomena observed in the OGT-KD cells.
34981577	7	14	theme	V.	1397:1398	arg1	blot					1408:1411	N-acetylglucosaminyltransferase V. Western blot	1365:1411	N-acetylglucosaminyltransferase V. Western blot	1365:1411	Furthermore, co-immunoprecipitation experiments have shown that SLC35A3 interacts with GnT-IV, but not with N-acetylglucosaminyltransferase V. Western blot and chemoenzymatic labeling assay have confirmed that OGT modifies SLC35A3 and that O-GlcNAcylation contributes to its stability.
34981577	1	15	theme	N-Linked	102:109	arg1	N-acetylglucosamine					138:156	O-linked N-acetylglucosamine	129:156	O-linked N-acetylglucosamine (O-GlcNAc)	129:167	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	15	theme	N-Linked	102:109	arg1	glycosylation					111:123	N-Linked glycosylation	102:123	N-Linked glycosylation	102:123	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	15	theme	N-Linked	102:109	arg1	modifications					210:222	important protein post-translational modifications	173:222	important protein post-translational modifications that are orchestrated by a diverse set of gene products	173:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	8	16	theme	cell	1644:1647	arg1	proliferation					1649:1661	cell proliferation	1644:1661	cell proliferation	1644:1661	Furthermore, we found that SLC35A3-KO enhances cell spreading and suppresses both cell migration and cell proliferation, which is similar to the phenomena observed in the OGT-KD cells.
34981577	4	17	theme	tetra-antennary	802:816	arg1	N-glycans					818:826	tetra-antennary N-glycans	802:826	tetra-antennary N-glycans	802:826	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	7	18	theme	chemoenzymatic	1417:1430	arg1	labeling					1432:1439	chemoenzymatic labeling	1417:1439	chemoenzymatic labeling assay	1417:1445	Furthermore, co-immunoprecipitation experiments have shown that SLC35A3 interacts with GnT-IV, but not with N-acetylglucosaminyltransferase V. Western blot and chemoenzymatic labeling assay have confirmed that OGT modifies SLC35A3 and that O-GlcNAcylation contributes to its stability.
34981577	7	19	theme	co-immunoprecipitation	1270:1291	arg1	experiments					1293:1303	co-immunoprecipitation experiments	1270:1303	co-immunoprecipitation experiments	1270:1303	Furthermore, co-immunoprecipitation experiments have shown that SLC35A3 interacts with GnT-IV, but not with N-acetylglucosaminyltransferase V. Western blot and chemoenzymatic labeling assay have confirmed that OGT modifies SLC35A3 and that O-GlcNAcylation contributes to its stability.
34981577	7	20	theme	Western	1400:1406	arg1	blot					1408:1411	N-acetylglucosaminyltransferase V. Western blot	1365:1411	N-acetylglucosaminyltransferase V. Western blot	1365:1411	Furthermore, co-immunoprecipitation experiments have shown that SLC35A3 interacts with GnT-IV, but not with N-acetylglucosaminyltransferase V. Western blot and chemoenzymatic labeling assay have confirmed that OGT modifies SLC35A3 and that O-GlcNAcylation contributes to its stability.
34981577	0	21	theme	N-glycan	47:54	arg1	biosynthesis					56:67	β1,4-GlcNAc-branched N-glycan biosynthesis	26:67	β1,4-GlcNAc-branched N-glycan biosynthesis	26:67	O-GlcNAcylation regulates β1,4-GlcNAc-branched N-glycan biosynthesis via the OGT/SLC35A3/GnT-IV axis.
34981577	1	22	theme	O-linked	129:136	arg1	modifications					210:222	important protein post-translational modifications	173:222	important protein post-translational modifications that are orchestrated by a diverse set of gene products	173:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	22	theme	O-linked	129:136	arg1	O-GlcNAc					159:166	O-GlcNAc	159:166	O-GlcNAc	159:166	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	22	theme	O-linked	129:136	arg1	glycosylation					111:123	N-Linked glycosylation	102:123	N-Linked glycosylation	102:123	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	22	theme	O-linked	129:136	arg1	N-acetylglucosamine					138:156	O-linked N-acetylglucosamine	129:156	O-linked N-acetylglucosamine (O-GlcNAc)	129:167	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	2	23	theme	donor	456:460	arg1	substrate					462:470	a common donor substrate	447:470	a common donor substrate	447:470	Thus far, the relationship between these two types of glycosylation has remained elusive, and it is unclear whether one influences the other via UDP-GlcNAc, which is a common donor substrate.
34981577	2	23	theme	donor	456:460	arg1	UDP-GlcNAc					426:435	UDP-GlcNAc	426:435	UDP-GlcNAc	426:435	Thus far, the relationship between these two types of glycosylation has remained elusive, and it is unclear whether one influences the other via UDP-GlcNAc, which is a common donor substrate.
34981577	7	24	theme	labeling	1432:1439	arg1	assay					1441:1445	chemoenzymatic labeling assay	1417:1445	chemoenzymatic labeling assay	1417:1445	Furthermore, co-immunoprecipitation experiments have shown that SLC35A3 interacts with GnT-IV, but not with N-acetylglucosaminyltransferase V. Western blot and chemoenzymatic labeling assay have confirmed that OGT modifies SLC35A3 and that O-GlcNAcylation contributes to its stability.
34981577	6	25	from	decrease	1178:1185	arg1	amounts					1194:1200	the amounts	1190:1200	the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans	1190:1254	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	6	25	from	decrease	1178:1185	arg1	N-glycans					1246:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	1	26	theme	diverse	251:257	arg1	set					259:261	a diverse set	249:261	a diverse set of gene products	249:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	26	theme	diverse	251:257	arg1	products					271:278	gene products	266:278	gene products	266:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	2	27	theme	common	449:454	arg1	substrate					462:470	a common donor substrate	447:470	a common donor substrate	447:470	Thus far, the relationship between these two types of glycosylation has remained elusive, and it is unclear whether one influences the other via UDP-GlcNAc, which is a common donor substrate.
34981577	2	27	theme	common	449:454	arg1	UDP-GlcNAc					426:435	UDP-GlcNAc	426:435	UDP-GlcNAc	426:435	Thus far, the relationship between these two types of glycosylation has remained elusive, and it is unclear whether one influences the other via UDP-GlcNAc, which is a common donor substrate.
34981577	3	28	theme	N-glycans	563:571	arg1	products					535:542	the products	531:542	the products of GlcNAc-branched N-glycans	531:571	Theoretically, a decrease in O-GlcNAcylation may increase the products of GlcNAc-branched N-glycans.
34981577	2	29	theme	glycosylation	335:347	arg1	types					326:330	these two types	316:330	these two types of glycosylation	316:347	Thus far, the relationship between these two types of glycosylation has remained elusive, and it is unclear whether one influences the other via UDP-GlcNAc, which is a common donor substrate.
34981577	4	30	theme	spectrometry	640:651	arg1	analysis					653:660	mass spectrometry analysis	635:660	mass spectrometry analysis	635:660	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	31	theme	transferase	869:879	arg1	OGT-KD					898:903	OGT-KD	898:903	OGT-KD	898:903	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	31	theme	transferase	869:879	arg1	cells					891:895	O-GlcNAc transferase knockdown cells	860:895	O-GlcNAc transferase knockdown cells (OGT-KD)	860:904	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	32	theme	O-GlcNAc	860:867	arg1	OGT-KD					898:903	OGT-KD	898:903	OGT-KD	898:903	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	32	theme	O-GlcNAc	860:867	arg1	cells					891:895	O-GlcNAc transferase knockdown cells	860:895	O-GlcNAc transferase knockdown cells (OGT-KD)	860:904	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	1	33	theme	gene	266:269	arg1	products					271:278	gene products	266:278	gene products	266:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	0	34	theme	OGT/SLC35A3/GnT-IV	77:94	arg1	axis					96:99	the OGT/SLC35A3/GnT-IV axis	73:99	the OGT/SLC35A3/GnT-IV axis	73:99	O-GlcNAcylation regulates β1,4-GlcNAc-branched N-glycan biosynthesis via the OGT/SLC35A3/GnT-IV axis.
34981577	4	35	theme	N-glycans	731:739	arg1	N-glycans					731:739	GlcNAc-branched tri-antennary N-glycans	701:739	GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV)	701:796	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	35	theme	N-glycans	731:739	arg1	amounts					690:696	the amounts	686:696	the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans	686:826	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	35	theme	N-glycans	731:739	arg1	N-glycans					818:826	tetra-antennary N-glycans	802:826	tetra-antennary N-glycans	802:826	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	36	theme	type	934:937	arg1	cells					939:943	wild type cells	929:943	wild type cells	929:943	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	37	theme	knockdown	881:889	arg1	OGT-KD					898:903	OGT-KD	898:903	OGT-KD	898:903	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	37	theme	knockdown	881:889	arg1	cells					891:895	O-GlcNAc transferase knockdown cells	860:895	O-GlcNAc transferase knockdown cells (OGT-KD)	860:904	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	38	theme	tri-antennary	717:729	arg1	N-glycans					731:739	GlcNAc-branched tri-antennary N-glycans	701:739	GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV)	701:796	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	6	39	theme	tetra-antennary	1230:1244	arg1	N-glycans					1246:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	5	40	theme	main	1020:1023	arg1	transporter					1036:1046	the main UDP-GlcNAc transporter	1016:1046	the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation	1016:1104	We examined this specific alteration by focusing on SLC35A3, which is the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation.
34981577	5	40	theme	main	1020:1023	arg1	SLC35A3					998:1004	SLC35A3	998:1004	SLC35A3	998:1004	We examined this specific alteration by focusing on SLC35A3, which is the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation.
34981577	3	41	from	decrease	490:497	arg1	O-GlcNAcylation					502:516	O-GlcNAcylation	502:516	O-GlcNAcylation	502:516	Theoretically, a decrease in O-GlcNAcylation may increase the products of GlcNAc-branched N-glycans.
34981577	5	42	theme	specific	963:970	arg1	alteration					972:981	this specific alteration	958:981	this specific alteration	958:981	We examined this specific alteration by focusing on SLC35A3, which is the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation.
34981577	1	43	theme	products	271:278	arg1	set					259:261	a diverse set	249:261	a diverse set of gene products	249:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	43	theme	products	271:278	arg1	products					271:278	gene products	266:278	gene products	266:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	4	44	theme	N-glycans	818:826	arg1	N-glycans					731:739	GlcNAc-branched tri-antennary N-glycans	701:739	GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV)	701:796	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	44	theme	N-glycans	818:826	arg1	amounts					690:696	the amounts	686:696	the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans	686:826	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	4	44	theme	N-glycans	818:826	arg1	N-glycans					818:826	tetra-antennary N-glycans	802:826	tetra-antennary N-glycans	802:826	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	5	45	theme	GnT-IV	1088:1093	arg1	activation					1095:1104	GnT-IV activation	1088:1104	GnT-IV activation	1088:1104	We examined this specific alteration by focusing on SLC35A3, which is the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation.
34981577	5	46	theme	UDP-GlcNAc	1025:1034	arg1	transporter					1036:1046	the main UDP-GlcNAc transporter	1016:1046	the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation	1016:1104	We examined this specific alteration by focusing on SLC35A3, which is the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation.
34981577	5	46	theme	UDP-GlcNAc	1025:1034	arg1	SLC35A3					998:1004	SLC35A3	998:1004	SLC35A3	998:1004	We examined this specific alteration by focusing on SLC35A3, which is the main UDP-GlcNAc transporter in mammals that is believed to modulate GnT-IV activation.
34981577	1	47	theme	important	173:181	arg1	N-acetylglucosamine					138:156	O-linked N-acetylglucosamine	129:156	O-linked N-acetylglucosamine (O-GlcNAc)	129:167	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	47	theme	important	173:181	arg1	glycosylation					111:123	N-Linked glycosylation	102:123	N-Linked glycosylation	102:123	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	47	theme	important	173:181	arg1	modifications					210:222	important protein post-translational modifications	173:222	important protein post-translational modifications that are orchestrated by a diverse set of gene products	173:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	6	48	theme	N-glycans	1246:1254	arg1	amounts					1194:1200	the amounts	1190:1200	the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans	1190:1254	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	6	48	theme	N-glycans	1246:1254	arg1	N-glycans					1246:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	1	49	theme	post-translational	191:208	arg1	N-acetylglucosamine					138:156	O-linked N-acetylglucosamine	129:156	O-linked N-acetylglucosamine (O-GlcNAc)	129:167	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	49	theme	post-translational	191:208	arg1	glycosylation					111:123	N-Linked glycosylation	102:123	N-Linked glycosylation	102:123	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	49	theme	post-translational	191:208	arg1	modifications					210:222	important protein post-translational modifications	173:222	important protein post-translational modifications that are orchestrated by a diverse set of gene products	173:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	50	theme	protein	183:189	arg1	N-acetylglucosamine					138:156	O-linked N-acetylglucosamine	129:156	O-linked N-acetylglucosamine (O-GlcNAc)	129:167	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	50	theme	protein	183:189	arg1	glycosylation					111:123	N-Linked glycosylation	102:123	N-Linked glycosylation	102:123	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	1	50	theme	protein	183:189	arg1	modifications					210:222	important protein post-translational modifications	173:222	important protein post-translational modifications that are orchestrated by a diverse set of gene products	173:278	N-Linked glycosylation and O-linked N-acetylglucosamine (O-GlcNAc) are important protein post-translational modifications that are orchestrated by a diverse set of gene products.
34981577	6	51	theme	GlcNAc-branched	1205:1219	arg1	N-glycans					1246:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	GlcNAc-branched tri- and tetra-antennary N-glycans	1205:1254	It is interesting that a deficiency of SLC35A3 specifically leads to a decrease in the amounts of GlcNAc-branched tri- and tetra-antennary N-glycans.
34981577	4	52	theme	mass	635:638	arg1	analysis					653:660	mass spectrometry analysis	635:660	mass spectrometry analysis	635:660	In this study, via examination by lectin blotting, HPLC, and mass spectrometry analysis, however, we found that the amounts of GlcNAc-branched tri-antennary N-glycans catalyzed by N-acetylglucosaminyltransferase IV (GnT-IV) and tetra-antennary N-glycans were significantly decreased in O-GlcNAc transferase knockdown cells (OGT-KD) compared with those in wild type cells.
34981577	9	53	theme	tetra-antennary	1855:1869	arg1	N-glycans					1871:1879	tri- and tetra-antennary N-glycans	1846:1879	tri- and tetra-antennary N-glycans	1846:1879	Taken together, these data are the first to demonstrate that O-GlcNAcylation specifically governs the biosynthesis of tri- and tetra-antennary N-glycans via the OGT-SLC35A3-GnT-IV axis.
34981577	0	54	theme	β1,4-GlcNAc-branched	26:45	arg1	biosynthesis					56:67	β1,4-GlcNAc-branched N-glycan biosynthesis	26:67	β1,4-GlcNAc-branched N-glycan biosynthesis	26:67	O-GlcNAcylation regulates β1,4-GlcNAc-branched N-glycan biosynthesis via the OGT/SLC35A3/GnT-IV axis.
34981577	3	55	theme	GlcNAc-branched	547:561	arg1	N-glycans					563:571	GlcNAc-branched N-glycans	547:571	GlcNAc-branched N-glycans	547:571	Theoretically, a decrease in O-GlcNAcylation may increase the products of GlcNAc-branched N-glycans.
34981577	9	56	theme	OGT-SLC35A3-GnT-IV	1889:1906	arg1	axis					1908:1911	the OGT-SLC35A3-GnT-IV axis	1885:1911	the OGT-SLC35A3-GnT-IV axis	1885:1911	Taken together, these data are the first to demonstrate that O-GlcNAcylation specifically governs the biosynthesis of tri- and tetra-antennary N-glycans via the OGT-SLC35A3-GnT-IV axis.
35077603	3	0	theme	bleeding	520:527	arg1	risk					529:532	high bleeding risk	515:532	a typical high bleeding risk cohort	505:539	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	7	1	theme	baseline	1080:1087	arg1	characteristics					1089:1103	balance baseline characteristics	1072:1103	balance baseline characteristics between groups	1072:1118	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	3	2	theme	world	550:554	arg1	practice					556:563	real world practice	545:563	real world practice	545:563	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	1	3	theme	coronary	298:305	arg1	PCI					321:323	PCI	321:323	PCI	321:323	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	1	3	theme	coronary	298:305	arg1	intervention					307:318	percutaneous coronary intervention	285:318	percutaneous coronary intervention (PCI)	285:324	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	6	4	theme	adverse	889:895	arg1	NACE					914:917	NACE	914:917	NACE	914:917	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	4	theme	adverse	889:895	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	4	theme	adverse	889:895	arg1	events					906:911	12-month net adverse clinical events	876:911	12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	876:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	9	5	theme	major	1501:1505	arg1	bleedings					1507:1515	major bleedings	1501:1515	major bleedings (0.5% vs. 0.5%, p = 1.000)	1501:1542	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	9	5	theme	major	1501:1505	arg1	%					1521:1521	0.5%	1518:1521	0.5%	1518:1521	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	9	5	theme	major	1501:1505	arg1	%					1530:1530	0.5%	1527:1530	0.5%	1527:1530	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	4	6	theme	elderly	668:674	arg1	patients					676:683	4736 consecutive elderly patients	651:683	4736 consecutive elderly patients who underwent PCI	651:701	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	6	theme	elderly	668:674	arg1	study					644:648	this single center, real-world observational study	599:648	study	644:648	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	6	7	theme	death	954:958	arg1	stroke					984:989	stroke	984:989	stroke	984:989	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	7	theme	death	954:958	arg1	bleeding					1018:1025	bleeding	1018:1025	bleeding	1018:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	7	theme	death	954:958	arg1	death					954:958	cardiac death	946:958	cardiac death	946:958	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	7	theme	death	954:958	arg1	revascularization					992:1008	revascularization	992:1008	revascularization	992:1008	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	7	theme	death	954:958	arg1	composite					933:941	a composite	931:941	a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	931:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	7	theme	death	954:958	arg1	infarction					972:981	myocardial infarction	961:981	myocardial infarction	961:981	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	4	8	theme	real-world	619:628	arg1	patients					676:683	4736 consecutive elderly patients	651:683	4736 consecutive elderly patients who underwent PCI	651:701	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	8	theme	real-world	619:628	arg1	study					644:648	this single center, real-world observational study	599:648	study	644:648	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	10	9	theme	heparin	1767:1773	arg1	monotherapy					1775:1785	heparin monotherapy	1767:1785	heparin monotherapy	1767:1785	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	1	10	theme	bivalirudin	208:218	arg1	safety					198:203	safety	198:203	safety	198:203	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	1	10	theme	bivalirudin	208:218	arg1	effectiveness					180:192	effectiveness	180:192	effectiveness	180:192	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	8	11	theme	lower	1184:1188	arg1	rates					1190:1194	lower rates	1184:1194	lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023)	1184:1320	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	9	12	located	found	1375:1379	arg1	incidences					1388:1397	the incidences	1384:1397	the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000)	1384:1542	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	9	12	located	found	1375:1379	arg2	differences					1358:1368	No differences	1355:1368	No differences	1355:1368	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	0	13	theme	real-world	138:147	arg1	study					149:153	A real-world study	136:153	A real-world study	136:153	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	10	14	dep	CONCLUSION	1576:1585	arg1	study					1620:1624	this real-world observational study	1590:1624	this real-world observational study	1590:1624	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	10	15	theme	increased	1796:1804	arg1	risk					1806:1809	increased risk	1796:1809	increased risk of stent thrombosis	1796:1829	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	7	16	theme	Propensity	1028:1037	arg1	score					1039:1043	Propensity score matching	1028:1052	Propensity score matching (PSM)	1028:1058	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	7	16	theme	Propensity	1028:1037	arg1	PSM					1055:1057	PSM	1055:1057	PSM	1055:1057	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	10	17	theme	real-world	1595:1604	arg1	study					1620:1624	this real-world observational study	1590:1624	this real-world observational study	1590:1624	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	8	18	theme	cardiac	1234:1240	arg1	%					1261:1261	4.3%	1258:1261	4.3%	1258:1261	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	8	18	theme	cardiac	1234:1240	arg1	death					1242:1246	cardiac death	1234:1246	cardiac death (2.7% vs. 4.3%, p = 0.038)	1234:1273	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	8	18	theme	cardiac	1234:1240	arg1	%					1252:1252	2.7%	1249:1252	2.7%	1249:1252	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	1	19	from	monotherapy	242:252	arg1	patients					265:272	elderly patients	257:272	elderly patients undergoing percutaneous coronary intervention (PCI)	257:324	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	8	20	dep	%	1298:1298	arg1	p = 0.023					1311:1319	p = 0.023	1311:1319	p = 0.023	1311:1319	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	10	21	theme	thrombosis	1820:1829	arg1	risk					1806:1809	increased risk	1796:1809	increased risk of stent thrombosis	1796:1829	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	10	22	theme	periprocedural	1627:1640	arg1	use					1642:1644	periprocedural use	1627:1644	periprocedural use of bivalirudin in elderly patients who underwent PCI	1627:1697	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	9	23	theme	patient	1560:1566	arg1	groups					1568:1573	the two patient groups	1552:1573	the two patient groups	1552:1573	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	0	24	from	safety	18:23	arg1	patients					51:58	elderly patients	43:58	elderly patients with coronary artery disease undergoing percutaneous coronary intervention	43:133	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	0	25	theme	artery	74:79	arg1	disease					81:87	coronary artery disease	65:87	coronary artery disease	65:87	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	0	26	from	Effectiveness	0:12	arg1	patients					51:58	elderly patients	43:58	elderly patients with coronary artery disease undergoing percutaneous coronary intervention	43:133	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	9	27	theme	infarction	1413:1422	arg1	incidences					1388:1397	the incidences	1384:1397	the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000)	1384:1542	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	6	28	theme	myocardial	961:970	arg1	infarction					972:981	myocardial infarction	961:981	myocardial infarction	961:981	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	2	29	theme	high	426:429	arg1	risk					440:443	high bleeding risk	426:443	high bleeding risk	426:443	BACKGROUND Bivalirudin is recommended for periprocedural use in patients undergoing PCI who are of high bleeding risk.
35077603	0	30	theme	coronary	113:120	arg1	intervention					122:133	percutaneous coronary intervention	100:133	percutaneous coronary intervention	100:133	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	9	31	theme	stroke	1425:1430	arg1	incidences					1388:1397	the incidences	1384:1397	the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000)	1384:1542	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	9	32	theme	revascularization	1433:1449	arg1	incidences					1388:1397	the incidences	1384:1397	the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000)	1384:1542	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	10	33	from	use	1642:1644	arg1	patients					1672:1679	elderly patients	1664:1679	elderly patients who underwent PCI	1664:1697	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	9	34	theme	thrombosis	1458:1467	arg1	incidences					1388:1397	the incidences	1384:1397	the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000)	1384:1542	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	5	35	theme	anticoagulation	817:831	arg1	strategies					833:842	the periprocedural anticoagulation strategies	798:842	the periprocedural anticoagulation strategies of PCI	798:849	Of these, 1240 were treated with bivalirudin and 3496 with heparin according to the periprocedural anticoagulation strategies of PCI.
35077603	9	36	theme	stent	1452:1456	arg1	thrombosis					1458:1467	stent thrombosis	1452:1467	stent thrombosis (0.1% vs. 0.1%, p = 1.000)	1452:1494	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	9	36	theme	stent	1452:1456	arg1	%					1473:1473	0.1%	1470:1473	0.1%	1470:1473	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	9	36	theme	stent	1452:1456	arg1	%					1482:1482	0.1%	1479:1482	0.1%	1479:1482	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	8	37	theme	bleeding	1284:1291	arg1	rates					1190:1194	lower rates	1184:1194	lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023)	1184:1320	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	3	38	theme	efficacious	468:478	arg1	use					480:482	its safe and efficacious use	455:482	its safe and efficacious use in elderly patients	455:502	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	3	38	theme	efficacious	468:478	arg1	cohort					534:539	a typical high bleeding risk cohort	505:539	a typical high bleeding risk cohort	505:539	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	0	39	theme	bivalirudin	28:38	arg1	safety					18:23	safety	18:23	safety	18:23	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	0	39	theme	bivalirudin	28:38	arg1	Effectiveness					0:12	Effectiveness	0:12	Effectiveness	0:12	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	6	40	theme	primary	856:862	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	40	theme	primary	856:862	arg1	events					906:911	12-month net adverse clinical events	876:911	12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	876:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	3	41	from	use	480:482	arg1	patients					495:502	elderly patients	487:502	elderly patients	487:502	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	3	41	from	use	480:482	arg1	practice					556:563	real world practice	545:563	real world practice	545:563	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	0	42	theme	elderly	43:49	arg1	patients					51:58	elderly patients	43:58	elderly patients with coronary artery disease undergoing percutaneous coronary intervention	43:133	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	1	43	theme	heparin	234:240	arg1	monotherapy					242:252	heparin monotherapy	234:252	heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI)	234:324	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	3	44	theme	risk	529:532	arg1	use					480:482	its safe and efficacious use	455:482	its safe and efficacious use in elderly patients	455:502	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	3	44	theme	risk	529:532	arg1	cohort					534:539	a typical high bleeding risk cohort	505:539	a typical high bleeding risk cohort	505:539	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	9	45	dep	%	1473:1473	arg1	p = 1.000					1485:1493	p = 1.000	1485:1493	p = 1.000	1485:1493	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	4	46	theme	single	604:609	arg1	patients					676:683	4736 consecutive elderly patients	651:683	4736 consecutive elderly patients who underwent PCI	651:701	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	46	theme	single	604:609	arg1	study					644:648	this single center, real-world observational study	599:648	study	644:648	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	10	47	theme	cardiac	1724:1730	arg1	death					1732:1736	cardiac death	1724:1736	cardiac death	1724:1736	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	3	48	theme	high	515:518	arg1	risk					529:532	high bleeding risk	515:532	a typical high bleeding risk cohort	505:539	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	7	49	theme	balance	1072:1078	arg1	characteristics					1089:1103	balance baseline characteristics	1072:1103	balance baseline characteristics between groups	1072:1118	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	3	50	theme	real	545:548	arg1	practice					556:563	real world practice	545:563	real world practice	545:563	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	1	51	theme	percutaneous	285:296	arg1	PCI					321:323	PCI	321:323	PCI	321:323	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	1	51	theme	percutaneous	285:296	arg1	intervention					307:318	percutaneous coronary intervention	285:318	percutaneous coronary intervention (PCI)	285:324	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	6	52	theme	clinical	897:904	arg1	NACE					914:917	NACE	914:917	NACE	914:917	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	52	theme	clinical	897:904	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	52	theme	clinical	897:904	arg1	events					906:911	12-month net adverse clinical events	876:911	12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	876:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	2	53	from	use	384:386	arg1	patients					391:398	patients	391:398	patients undergoing PCI who are of high bleeding risk	391:443	BACKGROUND Bivalirudin is recommended for periprocedural use in patients undergoing PCI who are of high bleeding risk.
35077603	7	54	used	used	1064:1067	arg2	PSM					1055:1057	PSM	1055:1057	PSM	1055:1057	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	7	54	used	used	1064:1067	arg2	score					1039:1043	Propensity score matching	1028:1052	Propensity score matching (PSM)	1028:1058	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	6	55	theme	net	885:887	arg1	NACE					914:917	NACE	914:917	NACE	914:917	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	55	theme	net	885:887	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	55	theme	net	885:887	arg1	events					906:911	12-month net adverse clinical events	876:911	12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	876:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	9	56	theme	bleedings	1507:1515	arg1	incidences					1388:1397	the incidences	1384:1397	the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000)	1384:1542	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	8	57	theme	NACE	1199:1202	arg1	rates					1190:1194	lower rates	1184:1194	lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023)	1184:1320	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	4	58	theme	consecutive	656:666	arg1	patients					676:683	4736 consecutive elderly patients	651:683	4736 consecutive elderly patients who underwent PCI	651:701	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	58	theme	consecutive	656:666	arg1	study					644:648	this single center, real-world observational study	599:648	study	644:648	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	2	59	theme	BACKGROUND	327:336	arg1	Bivalirudin					338:348	BACKGROUND Bivalirudin	327:348	BACKGROUND Bivalirudin	327:348	BACKGROUND Bivalirudin is recommended for periprocedural use in patients undergoing PCI who are of high bleeding risk.
35077603	6	60	theme	cardiac	946:952	arg1	death					954:958	cardiac death	946:958	cardiac death	946:958	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	2	61	theme	periprocedural	369:382	arg1	use					384:386	periprocedural use	369:386	periprocedural use in patients undergoing PCI who are of high bleeding risk	369:443	BACKGROUND Bivalirudin is recommended for periprocedural use in patients undergoing PCI who are of high bleeding risk.
35077603	5	62	theme	PCI	847:849	arg1	strategies					833:842	the periprocedural anticoagulation strategies	798:842	the periprocedural anticoagulation strategies of PCI	798:849	Of these, 1240 were treated with bivalirudin and 3496 with heparin according to the periprocedural anticoagulation strategies of PCI.
35077603	4	63	theme	observational	630:642	arg1	patients					676:683	4736 consecutive elderly patients	651:683	4736 consecutive elderly patients who underwent PCI	651:701	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	63	theme	observational	630:642	arg1	study					644:648	this single center, real-world observational study	599:648	study	644:648	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	64	from	METHODS	588:594	arg1	patients					676:683	4736 consecutive elderly patients	651:683	4736 consecutive elderly patients who underwent PCI	651:701	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	64	from	METHODS	588:594	arg1	study					644:648	this single center, real-world observational study	599:648	study	644:648	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	10	65	theme	observational	1606:1618	arg1	study					1620:1624	this real-world observational study	1590:1624	this real-world observational study	1590:1624	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	8	66	theme	heparin	1334:1340	arg1	monotherapy					1342:1352	heparin monotherapy	1334:1352	heparin monotherapy	1334:1352	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	10	67	theme	bivalirudin	1649:1659	arg1	use					1642:1644	periprocedural use	1627:1644	periprocedural use of bivalirudin in elderly patients who underwent PCI	1627:1697	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	0	68	theme	coronary	65:72	arg1	disease					81:87	coronary artery disease	65:87	coronary artery disease	65:87	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	0	69	dep	study	149:153	arg1	safety					18:23	safety	18:23	safety	18:23	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	0	69	dep	study	149:153	arg1	Effectiveness					0:12	Effectiveness	0:12	Effectiveness	0:12	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	6	70	theme	revascularization	992:1008	arg1	stroke					984:989	stroke	984:989	stroke	984:989	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	70	theme	revascularization	992:1008	arg1	bleeding					1018:1025	bleeding	1018:1025	bleeding	1018:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	70	theme	revascularization	992:1008	arg1	death					954:958	cardiac death	946:958	cardiac death	946:958	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	70	theme	revascularization	992:1008	arg1	revascularization					992:1008	revascularization	992:1008	revascularization	992:1008	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	70	theme	revascularization	992:1008	arg1	composite					933:941	a composite	931:941	a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	931:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	70	theme	revascularization	992:1008	arg1	infarction					972:981	myocardial infarction	961:981	myocardial infarction	961:981	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	8	71	theme	death	1242:1246	arg1	rates					1190:1194	lower rates	1184:1194	lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023)	1184:1320	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	10	72	theme	stent	1814:1818	arg1	thrombosis					1820:1829	stent thrombosis	1814:1829	stent thrombosis	1814:1829	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	9	73	dep	%	1521:1521	arg1	p = 1.000					1533:1541	p = 1.000	1533:1541	p = 1.000	1533:1541	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	6	74	theme	stroke	984:989	arg1	stroke					984:989	stroke	984:989	stroke	984:989	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	74	theme	stroke	984:989	arg1	bleeding					1018:1025	bleeding	1018:1025	bleeding	1018:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	74	theme	stroke	984:989	arg1	death					954:958	cardiac death	946:958	cardiac death	946:958	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	74	theme	stroke	984:989	arg1	revascularization					992:1008	revascularization	992:1008	revascularization	992:1008	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	74	theme	stroke	984:989	arg1	composite					933:941	a composite	931:941	a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	931:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	74	theme	stroke	984:989	arg1	infarction					972:981	myocardial infarction	961:981	myocardial infarction	961:981	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	0	75	theme	percutaneous	100:111	arg1	intervention					122:133	percutaneous coronary intervention	100:133	percutaneous coronary intervention	100:133	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	9	76	theme	myocardial	1402:1411	arg1	infarction					1413:1422	myocardial infarction	1402:1422	myocardial infarction	1402:1422	No differences were found in the incidences of myocardial infarction, stroke, revascularization, stent thrombosis (0.1% vs. 0.1%, p = 1.000), and major bleedings (0.5% vs. 0.5%, p = 1.000) between the two patient groups.
35077603	6	77	theme	infarction	972:981	arg1	stroke					984:989	stroke	984:989	stroke	984:989	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	77	theme	infarction	972:981	arg1	bleeding					1018:1025	bleeding	1018:1025	bleeding	1018:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	77	theme	infarction	972:981	arg1	death					954:958	cardiac death	946:958	cardiac death	946:958	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	77	theme	infarction	972:981	arg1	revascularization					992:1008	revascularization	992:1008	revascularization	992:1008	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	77	theme	infarction	972:981	arg1	composite					933:941	a composite	931:941	a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	931:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	77	theme	infarction	972:981	arg1	infarction					972:981	myocardial infarction	961:981	myocardial infarction	961:981	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	8	78	dep	RESULTS	1121:1127	arg1	found					1156:1160	found	1156:1160	was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy	1152:1352	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	8	79	dep	%	1209:1209	arg1	p = 0.002					1222:1230	p = 0.002	1222:1230	p = 0.002	1222:1230	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	5	80	theme	periprocedural	802:815	arg1	strategies					833:842	the periprocedural anticoagulation strategies	798:842	the periprocedural anticoagulation strategies of PCI	798:849	Of these, 1240 were treated with bivalirudin and 3496 with heparin according to the periprocedural anticoagulation strategies of PCI.
35077603	3	81	theme	elderly	487:493	arg1	patients					495:502	elderly patients	487:502	elderly patients	487:502	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	8	82	dep	%	1252:1252	arg1	p = 0.038					1264:1272	p = 0.038	1264:1272	p = 0.038	1264:1272	RESULTS After PSM, bivalirudin was found to be associated with lower rates of NACE (19.1% vs. 24.7%, p = 0.002), cardiac death (2.7% vs. 4.3%, p = 0.038), and any bleeding (10.0% vs. 12.9%, p = 0.023) compared to heparin monotherapy.
35077603	6	83	theme	bleeding	1018:1025	arg1	stroke					984:989	stroke	984:989	stroke	984:989	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	83	theme	bleeding	1018:1025	arg1	bleeding					1018:1025	bleeding	1018:1025	bleeding	1018:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	83	theme	bleeding	1018:1025	arg1	death					954:958	cardiac death	946:958	cardiac death	946:958	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	83	theme	bleeding	1018:1025	arg1	revascularization					992:1008	revascularization	992:1008	revascularization	992:1008	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	83	theme	bleeding	1018:1025	arg1	composite					933:941	a composite	931:941	a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding	931:1025	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	6	83	theme	bleeding	1018:1025	arg1	infarction					972:981	myocardial infarction	961:981	myocardial infarction	961:981	The primary outcome was 12-month net adverse clinical events (NACE) defined as a composite of cardiac death, myocardial infarction, stroke, revascularization, or any bleeding.
35077603	3	84	theme	safe	459:462	arg1	use					480:482	its safe and efficacious use	455:482	its safe and efficacious use in elderly patients	455:502	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	3	84	theme	safe	459:462	arg1	cohort					534:539	a typical high bleeding risk cohort	505:539	a typical high bleeding risk cohort	505:539	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	10	85	theme	elderly	1664:1670	arg1	patients					1672:1679	elderly patients	1664:1679	elderly patients who underwent PCI	1664:1697	CONCLUSION In this real-world observational study, periprocedural use of bivalirudin in elderly patients who underwent PCI was associated with less cardiac death and any bleeding compared to heparin monotherapy, without increased risk of stent thrombosis.
35077603	2	86	theme	bleeding	431:438	arg1	risk					440:443	high bleeding risk	426:443	high bleeding risk	426:443	BACKGROUND Bivalirudin is recommended for periprocedural use in patients undergoing PCI who are of high bleeding risk.
35077603	7	87	theme	matching	1045:1052	arg1	score					1039:1043	Propensity score matching	1028:1052	Propensity score matching (PSM)	1028:1058	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	7	87	theme	matching	1045:1052	arg1	PSM					1055:1057	PSM	1055:1057	PSM	1055:1057	Propensity score matching (PSM) was used to balance baseline characteristics between groups.
35077603	1	88	dep	effectiveness	180:192	arg1	the					176:178	the	176:178	the	176:178	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
35077603	3	89	theme	typical	507:513	arg1	use					480:482	its safe and efficacious use	455:482	its safe and efficacious use in elderly patients	455:502	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	3	89	theme	typical	507:513	arg1	cohort					534:539	a typical high bleeding risk cohort	505:539	a typical high bleeding risk cohort	505:539	However, its safe and efficacious use in elderly patients, a typical high bleeding risk cohort, in real world practice is yet to be reported.
35077603	0	90	with	patients	51:58	arg1	disease					81:87	coronary artery disease	65:87	coronary artery disease	65:87	Effectiveness and safety of bivalirudin in elderly patients with coronary artery disease undergoing percutaneous coronary intervention: A real-world study.
35077603	4	91	theme	center	611:616	arg1	patients					676:683	4736 consecutive elderly patients	651:683	4736 consecutive elderly patients who underwent PCI	651:701	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	4	91	theme	center	611:616	arg1	study					644:648	this single center, real-world observational study	599:648	study	644:648	METHODS In this single center, real-world observational study, 4736 consecutive elderly patients who underwent PCI were enrolled.
35077603	1	92	theme	elderly	257:263	arg1	patients					265:272	elderly patients	257:272	elderly patients undergoing percutaneous coronary intervention (PCI)	257:324	OBJECTIVE To assess the effectiveness and safety of bivalirudin compared with heparin monotherapy in elderly patients undergoing percutaneous coronary intervention (PCI).
36290595	2	0	dep	broilers	582:589	arg1	each					591:594	each	591:594	each	591:594	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	4	1	theme	ANT	1066:1068	arg1	group					1070:1074	ANT group	1066:1074	ANT group	1066:1074	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	3	2	theme	Terramycin	671:680	arg1	calcium					682:688	Terramycin calcium	671:688	Terramycin calcium	671:688	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	1	3	theme	supplementation	251:265	arg1	effects					232:238	the effects	228:238	the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers	228:444	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	8	4	theme	mg/kg	1679:1683	arg1	polysaccharides					1697:1711	150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1635:1711	150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1635:1711	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	6	5	theme	metabolomics	1280:1291	arg1	analysis					1293:1300	Serum metabolomics analysis	1274:1300	Serum metabolomics analysis	1274:1300	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	1	6	theme	compound	270:277	arg1	polysaccharides					279:293	compound polysaccharides	270:293	compound polysaccharides derived from Astragalus and Glycyrrhiza	270:333	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	2	7	dep	six	553:555	arg1	replicates					557:566	replicates	557:566	replicates	557:566	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	5	8	theme	HAG	1191:1193	arg1	supplementation					1195:1209	HAG supplementation	1191:1209	HAG supplementation	1191:1209	Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers.
36290595	8	9	theme	meat	1776:1779	arg1	quality					1781:1787	meat quality	1776:1787	meat quality	1776:1787	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	3	10	theme	Astragalus	733:742	arg1	polysaccharides					744:758	150 mg/kg Astragalus polysaccharides	723:758	150 mg/kg Astragalus polysaccharides	723:758	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	4	11	theme	LAG	938:940	arg1	supplementation					950:964	LAG and HAG supplementation	938:964	supplementation	950:964	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	0	12	theme	Antioxidant	116:126	arg1	Function					128:135	Antioxidant Function	116:135	Antioxidant Function	116:135	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	1	13	theme	cecal	394:398	arg1	microbiota					400:409	cecal microbiota	394:409	cecal microbiota	394:409	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	4	14	theme	HAG	946:948	arg1	supplementation					950:964	LAG and HAG supplementation	938:964	supplementation	950:964	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	4	15	theme	treatment	1111:1119	arg1	better					1125:1130	better	1125:1130	better	1125:1130	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	4	15	theme	treatment	1111:1119	arg1	effect					1097:1102	the effect	1093:1102	the effect of LAG treatment	1093:1119	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	3	16	theme	mg/kg	767:771	arg1	polysaccharides					785:799	75 mg/kg Glycyrrhiza polysaccharides	764:799	75 mg/kg Glycyrrhiza polysaccharides	764:799	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	6	17	theme	differential	1329:1340	arg1	metabolites					1342:1352	193 differential metabolites	1325:1352	193 differential metabolites	1325:1352	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	5	18	from	composition	1249:1259	arg1	broilers					1264:1271	broilers	1264:1271	broilers	1264:1271	Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers.
36290595	3	19	theme	HAG	802:804	arg1	group					806:810	HAG group	802:810	HAG group	802:810	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	5	20	from	diversity	1235:1243	arg1	broilers					1264:1271	broilers	1264:1271	broilers	1264:1271	Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers.
36290595	1	21	theme	broilers	437:444	arg1	performance					345:355	growth performance	338:355	growth performance	338:355	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	21	theme	broilers	437:444	arg1	quality					363:369	meat quality	358:369	meat quality	358:369	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	21	theme	broilers	437:444	arg1	function					384:391	antioxidant function	372:391	antioxidant function	372:391	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	21	theme	broilers	437:444	arg1	microbiota					400:409	cecal microbiota	394:409	cecal microbiota	394:409	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	21	theme	broilers	437:444	arg1	metabolomics					421:432	serum metabolomics	415:432	serum metabolomics	415:432	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	8	22	theme	cecal	1839:1843	arg1	composition					1856:1866	cecal microbiota composition	1839:1866	cecal microbiota composition	1839:1866	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	3	23	theme	mg/kg	838:842	arg1	polysaccharides					855:869	300 mg/kg Astragalus polysaccharides	834:869	300 mg/kg Astragalus polysaccharides	834:869	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	4	24	theme	antioxidant	996:1006	arg1	function					1008:1015	antioxidant function	996:1015	antioxidant function	996:1015	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	6	25	theme	acid	1432:1435	arg1	metabolism					1437:1446	linoleic acid metabolism	1423:1446	linoleic acid metabolism	1423:1446	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	3	26	theme	Glycyrrhiza	885:895	arg1	polysaccharides					897:911	150 mg/kg Glycyrrhiza polysaccharides	875:911	150 mg/kg Glycyrrhiza polysaccharides	875:911	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	2	27	theme	AA	492:493	arg1	broilers					496:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	0	28	theme	Serum	158:162	arg1	Metabolomics					164:175	Serum Metabolomics	158:175	Serum Metabolomics	158:175	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	29	theme	Growth	79:84	arg1	Performance					86:96	Growth Performance	79:96	Growth Performance	79:96	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	1	30	theme	meat	358:361	arg1	quality					363:369	meat quality	358:369	meat quality	358:369	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	0	31	from	Effects	0:6	arg1	Performance					86:96	Growth Performance	79:96	Growth Performance	79:96	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	31	from	Effects	0:6	arg1	Quality					104:110	Meat Quality	99:110	Meat Quality	99:110	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	31	from	Effects	0:6	arg1	Function					128:135	Antioxidant Function	116:135	Antioxidant Function	116:135	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	7	32	theme	phenotypic	1573:1582	arg1	indicators					1584:1593	phenotypic indicators	1573:1593	phenotypic indicators	1573:1593	Moreover, there was a close correlation between serum metabolites, cecal microbiota and phenotypic indicators.
36290595	5	33	theme	cecal	1219:1223	arg1	diversity					1235:1243	cecal microbial diversity	1219:1243	cecal microbial diversity	1219:1243	Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers.
36290595	4	34	theme	CON	1052:1054	arg1	group					1056:1060	the CON group	1048:1060	the CON group	1048:1060	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	2	35	theme	broilers	496:503	arg1	total					449:453	A total	447:453	A total of 480 one-day-old male Arbor Acres (AA) broilers	447:503	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	1	36	theme	polysaccharides	279:293	arg1	supplementation					251:265	dietary supplementation	243:265	dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza	243:333	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	0	37	theme	Compound	11:18	arg1	Polysaccharides					20:34	Compound Polysaccharides	11:34	Compound Polysaccharides Derived from Astragalus and Glycyrrhiza	11:74	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	7	38	theme	cecal	1552:1556	arg1	microbiota					1558:1567	cecal microbiota	1552:1567	cecal microbiota	1552:1567	Moreover, there was a close correlation between serum metabolites, cecal microbiota and phenotypic indicators.
36290595	2	39	theme	Acres	485:489	arg1	broilers					496:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	3	40	theme	ANT	639:641	arg1	group					643:647	ANT group	639:647	ANT group	639:647	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	4	41	theme	meat	1021:1024	arg1	quality					1026:1032	meat quality	1021:1032	meat quality	1021:1032	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	2	42	theme	male	474:477	arg1	broilers					496:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	5	43	theme	cecal	1154:1158	arg1	microbiota					1160:1169	cecal microbiota	1154:1169	cecal microbiota	1154:1169	Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers.
36290595	8	44	theme	Dietary	1608:1614	arg1	supplementation					1616:1630	Dietary supplementation	1608:1630	Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1608:1711	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	8	45	theme	Glycyrrhiza	1685:1695	arg1	polysaccharides					1697:1711	150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1635:1711	150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1635:1711	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	5	46	theme	microbial	1225:1233	arg1	diversity					1235:1243	cecal microbial diversity	1219:1243	cecal microbial diversity	1219:1243	Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers.
36290595	7	47	theme	close	1507:1511	arg1	correlation					1513:1523	a close correlation	1505:1523	a close correlation between serum metabolites, cecal microbiota and phenotypic indicators	1505:1593	Moreover, there was a close correlation between serum metabolites, cecal microbiota and phenotypic indicators.
36290595	8	48	theme	mg/kg	1639:1643	arg1	polysaccharides					1697:1711	150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1635:1711	150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1635:1711	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	3	49	theme	LAG	691:693	arg1	group					695:699	LAG group	691:699	LAG group	691:699	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	0	50	theme	Cecal	181:185	arg1	Microbiota					187:196	Cecal Microbiota	181:196	Cecal Microbiota	181:196	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	1	51	theme	dietary	243:249	arg1	supplementation					251:265	dietary supplementation	243:265	dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza	243:333	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	52	attach	derived	295:301	arg2	polysaccharides					279:293	compound polysaccharides	270:293	compound polysaccharides derived from Astragalus and Glycyrrhiza	270:333	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	52	attach	derived	295:301	arg1	Astragalus					308:317	Astragalus	308:317	Astragalus	308:317	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	52	attach	derived	295:301	arg1	Glycyrrhiza					323:333	Glycyrrhiza	323:333	Glycyrrhiza	323:333	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	8	53	theme	microbiota	1845:1854	arg1	composition					1856:1866	cecal microbiota composition	1839:1866	cecal microbiota composition	1839:1866	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	6	54	theme	Serum	1274:1278	arg1	analysis					1293:1300	Serum metabolomics analysis	1274:1300	Serum metabolomics analysis	1274:1300	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	8	55	theme	antioxidant	1751:1761	arg1	function					1763:1770	antioxidant function	1751:1770	antioxidant function	1751:1770	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	0	56	attach	Derived	36:42	arg1	Astragalus					49:58	Astragalus	49:58	Astragalus	49:58	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	56	attach	Derived	36:42	arg1	Glycyrrhiza					64:74	Glycyrrhiza	64:74	Glycyrrhiza	64:74	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	56	attach	Derived	36:42	arg2	Polysaccharides					20:34	Compound Polysaccharides	11:34	Compound Polysaccharides Derived from Astragalus and Glycyrrhiza	11:74	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	4	57	theme	LAG	1107:1109	arg1	treatment					1111:1119	LAG treatment	1107:1119	LAG treatment	1107:1119	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	3	58	theme	mg/kg	727:731	arg1	polysaccharides					744:758	150 mg/kg Astragalus polysaccharides	723:758	150 mg/kg Astragalus polysaccharides	723:758	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	6	59	theme	metabolites	1342:1352	arg1	total					1316:1320	a total	1314:1320	a total of 193 differential metabolites	1314:1352	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	8	60	theme	broilers	1792:1799	arg1	performance					1738:1748	the growth performance	1727:1748	the growth performance	1727:1748	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	8	60	theme	broilers	1792:1799	arg1	function					1763:1770	antioxidant function	1751:1770	antioxidant function	1751:1770	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	8	60	theme	broilers	1792:1799	arg1	quality					1781:1787	meat quality	1776:1787	meat quality	1776:1787	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	2	61	with	treatments	537:546	arg1	six					553:555	six	553:555	six	553:555	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	1	62	theme	growth	338:343	arg1	performance					345:355	growth performance	338:355	growth performance	338:355	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	0	63	theme	Meat	99:102	arg1	Quality					104:110	Meat Quality	99:110	Meat Quality	99:110	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	1	64	from	effects	232:238	arg1	performance					345:355	growth performance	338:355	growth performance	338:355	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	64	from	effects	232:238	arg1	quality					363:369	meat quality	358:369	meat quality	358:369	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	64	from	effects	232:238	arg1	function					384:391	antioxidant function	372:391	antioxidant function	372:391	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	64	from	effects	232:238	arg1	microbiota					400:409	cecal microbiota	394:409	cecal microbiota	394:409	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	64	from	effects	232:238	arg1	metabolomics					421:432	serum metabolomics	415:432	serum metabolomics	415:432	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	1	65	theme	serum	415:419	arg1	metabolomics					421:432	serum metabolomics	415:432	serum metabolomics	415:432	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	8	66	theme	serum	1817:1821	arg1	metabolites					1823:1833	the serum metabolites	1813:1833	the serum metabolites	1813:1833	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	3	67	theme	CON	609:611	arg1	Treatments					597:606	Treatments	597:606	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.	597:912	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	3	67	theme	CON	609:611	arg1	group					613:617	CON group	609:617	CON group	609:617	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	3	67	theme	CON	609:611	arg1	diet					633:636	the basal diet	623:636	the basal diet	623:636	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	3	68	theme	Astragalus	844:853	arg1	polysaccharides					855:869	300 mg/kg Astragalus polysaccharides	834:869	300 mg/kg Astragalus polysaccharides	834:869	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	4	69	dep	better	1125:1130	arg1	increased					966:974	increased	966:974	increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group	966:1074	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	6	70	theme	LAG	1381:1383	arg1	groups					1385:1390	CON and LAG groups	1373:1390	groups	1385:1390	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	3	71	theme	mg/kg	879:883	arg1	polysaccharides					897:911	150 mg/kg Glycyrrhiza polysaccharides	875:911	150 mg/kg Glycyrrhiza polysaccharides	875:911	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	6	72	theme	CON	1373:1375	arg1	groups					1385:1390	CON and LAG groups	1373:1390	groups	1385:1390	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	1	73	theme	antioxidant	372:382	arg1	function					384:391	antioxidant function	372:391	antioxidant function	372:391	This study aimed to evaluate the effects of dietary supplementation of compound polysaccharides derived from Astragalus and Glycyrrhiza on growth performance, meat quality, antioxidant function, cecal microbiota and serum metabolomics of broilers.
36290595	4	74	theme	growth	976:981	arg1	performance					983:993	growth performance	976:993	growth performance	976:993	The results showed that LAG and HAG supplementation increased growth performance, antioxidant function and meat quality compared with the CON group and ANT group and, especially, the effect of LAG treatment was better than HAG.
36290595	6	75	theme	glutathione	1452:1462	arg1	metabolism					1464:1473	glutathione metabolism	1452:1473	glutathione metabolism	1452:1473	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	8	76	dep	mg/kg	1639:1643	arg1	polysaccharides					1656:1670	Astragalus polysaccharides	1645:1670	150 mg/kg Astragalus polysaccharides	1635:1670	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	0	77	theme	Broilers	140:147	arg1	Performance					86:96	Growth Performance	79:96	Growth Performance	79:96	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	77	theme	Broilers	140:147	arg1	Quality					104:110	Meat Quality	99:110	Meat Quality	99:110	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	77	theme	Broilers	140:147	arg1	Function					128:135	Antioxidant Function	116:135	Antioxidant Function	116:135	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	0	78	theme	Polysaccharides	20:34	arg1	Effects					0:6	Effects	0:6	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers	0:147	Effects of Compound Polysaccharides Derived from Astragalus and Glycyrrhiza on Growth Performance, Meat Quality and Antioxidant Function of Broilers Based on Serum Metabolomics and Cecal Microbiota.
36290595	6	79	theme	linoleic	1423:1430	arg1	acid					1432:1435	linoleic acid	1423:1435	linoleic acid metabolism	1423:1446	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	2	80	theme	Arbor	479:483	arg1	broilers					496:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	3	81	theme	basal	627:631	arg1	Treatments					597:606	Treatments	597:606	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.	597:912	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	3	81	theme	basal	627:631	arg1	group					613:617	CON group	609:617	CON group	609:617	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	3	81	theme	basal	627:631	arg1	diet					633:636	the basal diet	623:636	the basal diet	623:636	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36290595	6	82	dep	metabolism	1437:1446	arg1	pathways					1475:1482	pathways	1475:1482	pathways	1475:1482	Serum metabolomics analysis showed that a total of 193 differential metabolites were identified in CON and LAG groups, which were mainly enriched in linoleic acid metabolism and glutathione metabolism pathways.
36290595	2	83	theme	one-day-old	462:472	arg1	broilers					496:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	480 one-day-old male Arbor Acres (AA) broilers	458:503	A total of 480 one-day-old male Arbor Acres (AA) broilers were randomly divided into four treatments with six replicates comprising 20 broilers each.
36290595	8	84	theme	polysaccharides	1697:1711	arg1	supplementation					1616:1630	Dietary supplementation	1608:1630	Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides	1608:1711	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	5	85	theme	microbiota	1160:1169	arg1	Analysis					1142:1149	Analysis	1142:1149	Analysis of cecal microbiota	1142:1169	Analysis of cecal microbiota showed that LAG and HAG supplementation altered cecal microbial diversity and composition in broilers.
36290595	7	86	theme	serum	1533:1537	arg1	metabolites					1539:1549	serum metabolites	1533:1549	serum metabolites	1533:1549	Moreover, there was a close correlation between serum metabolites, cecal microbiota and phenotypic indicators.
36290595	8	87	theme	Astragalus	1645:1654	arg1	polysaccharides					1656:1670	Astragalus polysaccharides	1645:1670	150 mg/kg Astragalus polysaccharides	1635:1670	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	8	88	theme	growth	1731:1736	arg1	performance					1738:1748	the growth performance	1727:1748	the growth performance	1727:1748	Conclusion: Dietary supplementation of 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides could improve the growth performance, antioxidant function and meat quality of broilers by changing the serum metabolites and cecal microbiota composition.
36290595	3	89	theme	Glycyrrhiza	773:783	arg1	polysaccharides					785:799	75 mg/kg Glycyrrhiza polysaccharides	764:799	75 mg/kg Glycyrrhiza polysaccharides	764:799	Treatments: CON group was the basal diet; ANT group was supplemented with Terramycin calcium; LAG group was supplemented with 150 mg/kg Astragalus polysaccharides and 75 mg/kg Glycyrrhiza polysaccharides; HAG group was supplemented with 300 mg/kg Astragalus polysaccharides and 150 mg/kg Glycyrrhiza polysaccharides.
36440681	13	0	theme	barrier	2064:2070	arg1	damage					2072:2077	Intestinal mucosal barrier damage	2045:2077	Intestinal mucosal barrier damage of b2KO mice	2045:2090	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	14	1	theme	intestinal	2295:2304	arg1	flora					2306:2310	the intestinal flora	2291:2310	the intestinal flora	2291:2310	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	5	2	theme	protective	624:633	arg1	effect					635:640	a protective effect	622:640	a protective effect	622:640	MRGPRB2 may have a protective effect on DSS-induced colitis.
36440681	6	3	theme	intestinal	755:764	arg1	microbiota					766:775	the intestinal microbiota	751:775	the intestinal microbiota	751:775	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	10	4	theme	colon	1590:1594	arg1	shortening					1596:1605	colon shortening	1590:1605	colon shortening	1590:1605	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	9	5	theme	male	1316:1319	arg1	mice					1321:1324	wild-type (WT) male mice	1301:1324	wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis	1301:1374	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	13	6	theme	mucin-2	2165:2171	arg1	expression					2151:2160	the attenuated expression	2136:2160	the attenuated expression of mucin-2 and occludin	2136:2184	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	14	7	theme	DSS-induced	2259:2269	arg1	colitis					2271:2277	DSS-induced colitis	2259:2277	DSS-induced colitis	2259:2277	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	10	8	theme	colonic	1611:1617	arg1	damage					1632:1637	colonic pathological damage	1611:1637	colonic pathological damage	1611:1637	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	8	9	theme	immune	1109:1114	arg1	cells					1116:1120	immune cells	1109:1120	immune cells	1109:1120	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	14	10	from	effect	2249:2254	arg1	colitis					2271:2277	DSS-induced colitis	2259:2277	DSS-induced colitis	2259:2277	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	9	11	from	differences	1243:1253	arg1	degree					1446:1451	degree	1446:1451	degree of intestinal barrier damage	1446:1480	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	11	from	differences	1243:1253	arg1	composition					1486:1496	composition	1486:1496	composition of the intestinal flora	1486:1520	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	11	from	differences	1243:1253	arg1	infiltration					1432:1443	inflammatory cell infiltration	1414:1443	inflammatory cell infiltration	1414:1443	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	11	from	differences	1243:1253	arg1	severity					1383:1390	the severity	1379:1390	the severity of clinical symptoms	1379:1411	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	2	12	from	barrier	338:344	arg1	colitis					282:288	ulcerative colitis	271:288	ulcerative colitis in relation to the intestinal flora	271:324	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	4	13	theme	barrier	596:602	arg1	disruption					567:576	disruption	567:576	disruption of the intestinal barrier	567:602	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	4	13	theme	barrier	596:602	arg1	microbiota					532:541	altered gut microbiota	520:541	altered gut microbiota	520:541	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	4	13	theme	barrier	596:602	arg1	response					554:561	immune response	547:561	immune response	547:561	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	0	14	theme	gut	115:117	arg1	microbiota					119:128	gut microbiota	115:128	gut microbiota	115:128	Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota.
36440681	2	15	theme	ulcerative	271:280	arg1	colitis					282:288	ulcerative colitis	271:288	ulcerative colitis in relation to the intestinal flora	271:324	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	9	16	from	UC	1215:1216	arg1	role					1196:1199	the role	1192:1199	the role of Mrgprb2 in UC in mice	1192:1224	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	16	from	UC	1215:1216	arg1	mice					1221:1224	mice	1221:1224	mice	1221:1224	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	17	dep	wild-type	1301:1309	arg1	WT					1312:1313	WT	1312:1313	WT	1312:1313	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	12	18	theme	bacteria	2009:2016	arg1	increase					1989:1996	increase	1989:1996	increase of harmful bacteria like Escherichia-Shigella	1989:2042	B2KO mice also exhibited a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus and increase of harmful bacteria like Escherichia-Shigella.
36440681	12	18	theme	bacteria	2009:2016	arg1	reduction					1911:1919	a reduction	1909:1919	a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus	1909:1983	B2KO mice also exhibited a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus and increase of harmful bacteria like Escherichia-Shigella.
36440681	6	19	from	damage	781:786	arg1	microbiota					766:775	the intestinal microbiota	751:775	the intestinal microbiota	751:775	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	10	20	theme	MC	1687:1688	arg1	activation					1690:1699	MC activation	1687:1699	MC activation	1687:1699	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	9	21	from	Mrgprb2	1204:1210	arg1	UC					1215:1216	UC	1215:1216	UC in mice	1215:1224	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	4	22	theme	mouse	424:428	arg1	Mrgprb2					430:436	mouse Mrgprb2	424:436	mouse Mrgprb2	424:436	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	5	23	theme	DSS-induced	645:655	arg1	colitis					657:663	DSS-induced colitis	645:663	DSS-induced colitis	645:663	MRGPRB2 may have a protective effect on DSS-induced colitis.
36440681	2	24	theme	X2	247:248	arg1	What					188:191	What	188:191	What	188:191	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	24	theme	X2	247:248	arg1	barrier					338:344	intestinal barrier	327:344	intestinal barrier	327:344	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	24	theme	X2	247:248	arg1	role					200:203	the role	196:203	the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora	196:324	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	24	theme	X2	247:248	arg1	response					357:364	immune response	350:364	immune response	350:364	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	25	theme	receptor	238:245	arg1	MRGPRX2/Mrgprb2					251:265	MRGPRX2/Mrgprb2	251:265	MRGPRX2/Mrgprb2	251:265	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	25	theme	receptor	238:245	arg1	X2					247:248	mas-related G protein-coupled receptor X2	208:248	mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2)	208:266	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	1	26	theme	NEW	131:133	arg1	question					164:171	the central question	152:171	the central question of this study	152:185	NEW FINDINGS What is the central question of this study?
36440681	1	26	theme	NEW	131:133	arg1	FINDINGS					135:142	NEW FINDINGS	131:142	NEW FINDINGS What is the central question of this study	131:185	NEW FINDINGS What is the central question of this study?
36440681	12	27	theme	probiotics	1924:1933	arg1	increase					1989:1996	increase	1989:1996	increase of harmful bacteria like Escherichia-Shigella	1989:2042	B2KO mice also exhibited a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus and increase of harmful bacteria like Escherichia-Shigella.
36440681	12	27	theme	probiotics	1924:1933	arg1	reduction					1911:1919	a reduction	1909:1919	a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus	1909:1983	B2KO mice also exhibited a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus and increase of harmful bacteria like Escherichia-Shigella.
36440681	0	28	theme	altered	58:64	arg1	response					73:80	altered immune response	58:80	altered immune response	58:80	Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota.
36440681	9	29	theme	Mrgprb2	1204:1210	arg1	role					1196:1199	the role	1192:1199	the role of Mrgprb2 in UC in mice	1192:1224	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	13	30	theme	attenuated	2140:2149	arg1	expression					2151:2160	the attenuated expression	2136:2160	the attenuated expression of mucin-2 and occludin	2136:2184	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	2	31	from	colitis	282:288	arg1	relation					293:300	relation	293:300	relation to the intestinal flora	293:324	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	9	32	theme	symptoms	1404:1411	arg1	degree					1446:1451	degree	1446:1451	degree of intestinal barrier damage	1446:1480	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	32	theme	symptoms	1404:1411	arg1	infiltration					1432:1443	inflammatory cell infiltration	1414:1443	inflammatory cell infiltration	1414:1443	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	32	theme	symptoms	1404:1411	arg1	composition					1486:1496	composition	1486:1496	composition of the intestinal flora	1486:1520	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	32	theme	symptoms	1404:1411	arg1	severity					1383:1390	the severity	1379:1390	the severity of clinical symptoms	1379:1411	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	11	33	theme	b2KO	1873:1876	arg1	mice					1878:1881	b2KO mice	1873:1881	b2KO mice	1873:1881	In addition, the abundance and diversity of the intestinal microbiota were reduced in b2KO mice.
36440681	0	34	theme	intestinal	83:92	arg1	function					102:109	intestinal barrier function	83:109	intestinal barrier function	83:109	Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota.
36440681	8	35	theme	Human	973:977	arg1	MRGPRX2					1022:1028	MRGPRX2	1022:1028	MRGPRX2	1022:1028	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	8	35	theme	Human	973:977	arg1	X2					1018:1019	Human mas-related G protein-coupled receptor X2	973:1019	Human mas-related G protein-coupled receptor X2 (MRGPRX2)	973:1029	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	7	36	theme	gastrointestinal	893:908	arg1	tract					910:914	the gastrointestinal tract	889:914	the gastrointestinal tract	889:914	Mast cells (MCs) are widely distributed in the gastrointestinal tract and are thought to be related to the pathogenesis of UC.
36440681	9	37	theme	inflammatory	1414:1425	arg1	infiltration					1432:1443	inflammatory cell infiltration	1414:1443	inflammatory cell infiltration	1414:1443	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	2	38	from	role	200:203	arg1	colitis					282:288	ulcerative colitis	271:288	ulcerative colitis in relation to the intestinal flora	271:324	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	6	39	theme	Ulcerative	675:684	arg1	disease					727:733	a chronic immune-related disease	702:733	a chronic immune-related disease	702:733	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	6	39	theme	Ulcerative	675:684	arg1	UC					695:696	UC	695:696	UC	695:696	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	6	39	theme	Ulcerative	675:684	arg1	colitis					686:692	ABSTRACT Ulcerative colitis	666:692	ABSTRACT Ulcerative colitis (UC)	666:697	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	13	40	theme	WT	2121:2122	arg1	mice					2124:2127	WT mice	2121:2127	WT mice	2121:2127	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	8	41	theme	receptor	1009:1016	arg1	MRGPRX2					1022:1028	MRGPRX2	1022:1028	MRGPRX2	1022:1028	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	8	41	theme	receptor	1009:1016	arg1	X2					1018:1019	Human mas-related G protein-coupled receptor X2	973:1019	Human mas-related G protein-coupled receptor X2 (MRGPRX2)	973:1029	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	14	42	contain	have	2231:2234	arg2	effect					2249:2254	a protective effect	2236:2254	a protective effect on DSS-induced colitis	2236:2277	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	14	42	contain	have	2231:2234	arg1	MRGPRB2					2219:2225	MRGPRB2	2219:2225	MRGPRB2	2219:2225	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	2	43	theme	protein-coupled	222:236	arg1	MRGPRX2/Mrgprb2					251:265	MRGPRX2/Mrgprb2	251:265	MRGPRX2/Mrgprb2	251:265	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	43	theme	protein-coupled	222:236	arg1	X2					247:248	mas-related G protein-coupled receptor X2	208:248	mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2)	208:266	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	9	44	theme	intestinal	1456:1465	arg1	damage					1475:1480	intestinal barrier damage	1456:1480	intestinal barrier damage	1456:1480	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	3	45	theme	main	379:382	arg1	What					367:370	What	367:370	What	367:370	What is the main finding and its importance?
36440681	3	45	theme	main	379:382	arg1	finding					384:390	the main finding	375:390	the main finding	375:390	What is the main finding and its importance?
36440681	4	46	theme	altered	520:526	arg1	microbiota					532:541	altered gut microbiota	520:541	altered gut microbiota	520:541	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	10	47	theme	weight	1547:1552	arg1	loss					1554:1557	weight loss	1547:1557	weight loss	1547:1557	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	0	48	theme	knockout	30:37	arg1	Mrgprb2					22:28	Mrgprb2 knockout	22:37	Mrgprb2 knockout	22:37	Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota.
36440681	9	49	theme	knockout	1271:1278	arg1	mice					1292:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	50	theme	damage	1475:1480	arg1	degree					1446:1451	degree	1446:1451	degree of intestinal barrier damage	1446:1480	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	50	theme	damage	1475:1480	arg1	infiltration					1432:1443	inflammatory cell infiltration	1414:1443	inflammatory cell infiltration	1414:1443	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	50	theme	damage	1475:1480	arg1	composition					1486:1496	composition	1486:1496	composition of the intestinal flora	1486:1520	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	50	theme	damage	1475:1480	arg1	severity					1383:1390	the severity	1379:1390	the severity of clinical symptoms	1379:1411	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	2	51	from	response	357:364	arg1	colitis					282:288	ulcerative colitis	271:288	ulcerative colitis in relation to the intestinal flora	271:324	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	6	52	from	changes	740:746	arg1	microbiota					766:775	the intestinal microbiota	751:775	the intestinal microbiota	751:775	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	6	53	theme	immune-related	712:725	arg1	colitis					686:692	ABSTRACT Ulcerative colitis	666:692	ABSTRACT Ulcerative colitis (UC)	666:697	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	6	53	theme	immune-related	712:725	arg1	disease					727:733	a chronic immune-related disease	702:733	a chronic immune-related disease	702:733	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	9	54	theme	b2KO	1281:1284	arg1	mice					1292:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	6	55	theme	intestinal	795:804	arg1	barrier					806:812	the intestinal barrier	791:812	the intestinal barrier	791:812	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	9	56	with	mice	1321:1324	arg1	colitis					1368:1374	dextran sulfate sodium (DSS)-induced colitis	1331:1374	dextran sulfate sodium (DSS)-induced colitis	1331:1374	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	10	57	theme	activity	1568:1575	arg1	score					1583:1587	disease activity index score	1560:1587	disease activity index score	1560:1587	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	9	58	theme	male	1287:1290	arg1	mice					1292:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	11	59	dep	abundance	1804:1812	arg1	the					1800:1802	the	1800:1802	the	1800:1802	In addition, the abundance and diversity of the intestinal microbiota were reduced in b2KO mice.
36440681	10	60	theme	cell	1753:1756	arg1	infiltration					1758:1769	inflammatory cell infiltration	1740:1769	inflammatory cell infiltration	1740:1769	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	9	61	theme	flora	1516:1520	arg1	degree					1446:1451	degree	1446:1451	degree of intestinal barrier damage	1446:1480	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	61	theme	flora	1516:1520	arg1	infiltration					1432:1443	inflammatory cell infiltration	1414:1443	inflammatory cell infiltration	1414:1443	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	61	theme	flora	1516:1520	arg1	composition					1486:1496	composition	1486:1496	composition of the intestinal flora	1486:1520	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	61	theme	flora	1516:1520	arg1	severity					1383:1390	the severity	1379:1390	the severity of clinical symptoms	1379:1411	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	62	with	mice	1292:1295	arg1	colitis					1368:1374	dextran sulfate sodium (DSS)-induced colitis	1331:1374	dextran sulfate sodium (DSS)-induced colitis	1331:1374	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	2	63	theme	intestinal	327:336	arg1	barrier					338:344	intestinal barrier	327:344	intestinal barrier	327:344	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	13	64	theme	mucosal	2056:2062	arg1	damage					2072:2077	Intestinal mucosal barrier damage	2045:2077	Intestinal mucosal barrier damage of b2KO mice	2045:2090	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	4	65	theme	immune	547:552	arg1	response					554:561	immune response	547:561	immune response	547:561	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	10	66	theme	pathological	1619:1630	arg1	damage					1632:1637	colonic pathological damage	1611:1637	colonic pathological damage	1611:1637	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	1	67	theme	central	156:162	arg1	question					164:171	the central question	152:171	the central question of this study	152:185	NEW FINDINGS What is the central question of this study?
36440681	1	67	theme	central	156:162	arg1	FINDINGS					135:142	NEW FINDINGS	131:142	NEW FINDINGS What is the central question of this study	131:185	NEW FINDINGS What is the central question of this study?
36440681	12	68	theme	B2KO	1884:1887	arg1	mice					1889:1892	B2KO mice	1884:1892	B2KO mice	1884:1892	B2KO mice also exhibited a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus and increase of harmful bacteria like Escherichia-Shigella.
36440681	14	69	theme	inflammatory	2360:2371	arg1	cells					2373:2377	inflammatory cells	2360:2377	inflammatory cells	2360:2377	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	8	70	theme	microbial	1156:1164	arg1	infection					1166:1174	microbial infection	1156:1174	microbial infection	1156:1174	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	10	71	theme	b2KO	1671:1674	arg1	mice					1676:1679	b2KO mice	1671:1679	b2KO mice	1671:1679	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	14	72	theme	protective	2238:2247	arg1	effect					2249:2254	a protective effect	2236:2254	a protective effect on DSS-induced colitis	2236:2277	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	1	73	theme	study	181:185	arg1	question					164:171	the central question	152:171	the central question of this study	152:185	NEW FINDINGS What is the central question of this study?
36440681	1	73	theme	study	181:185	arg1	FINDINGS					135:142	NEW FINDINGS	131:142	NEW FINDINGS What is the central question of this study	131:185	NEW FINDINGS What is the central question of this study?
36440681	14	74	theme	barrier	2330:2336	arg1	repair					2338:2343	barrier repair	2330:2343	barrier repair	2330:2343	These results demonstrated that MRGPRB2 may have a protective effect on DSS-induced colitis by altering the intestinal flora, participating in barrier repair and recruiting inflammatory cells to eliminate pathogens.
36440681	4	75	theme	intestinal	585:594	arg1	barrier					596:602	the intestinal barrier	581:602	the intestinal barrier	581:602	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	2	76	theme	intestinal	309:318	arg1	flora					320:324	the intestinal flora	305:324	the intestinal flora	305:324	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	77	theme	mas-related	208:218	arg1	MRGPRX2/Mrgprb2					251:265	MRGPRX2/Mrgprb2	251:265	MRGPRX2/Mrgprb2	251:265	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	2	77	theme	mas-related	208:218	arg1	X2					247:248	mas-related G protein-coupled receptor X2	208:248	mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2)	208:266	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	4	78	theme	Mrgprb2	430:436	arg1	Knockout					412:419	Knockout	412:419	Knockout	412:419	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	12	79	theme	harmful	2001:2007	arg1	bacteria					2009:2016	harmful bacteria	2001:2016	harmful bacteria like Escherichia-Shigella	2001:2042	B2KO mice also exhibited a reduction of probiotics such as norank_f_Muribaculaceae and Lactobacillus and increase of harmful bacteria like Escherichia-Shigella.
36440681	13	80	theme	Intestinal	2045:2054	arg1	damage					2072:2077	Intestinal mucosal barrier damage	2045:2077	Intestinal mucosal barrier damage of b2KO mice	2045:2090	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	9	81	theme	-induced	1359:1366	arg1	colitis					1368:1374	dextran sulfate sodium (DSS)-induced colitis	1331:1374	dextran sulfate sodium (DSS)-induced colitis	1331:1374	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	7	82	theme	UC	969:970	arg1	pathogenesis					953:964	the pathogenesis	949:964	the pathogenesis of UC	949:970	Mast cells (MCs) are widely distributed in the gastrointestinal tract and are thought to be related to the pathogenesis of UC.
36440681	10	83	theme	chemokine	1715:1723	arg1	secretion					1725:1733	cytokine and chemokine secretion	1702:1733	cytokine and chemokine secretion	1702:1733	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	13	84	theme	occludin	2177:2184	arg1	expression					2151:2160	the attenuated expression	2136:2160	the attenuated expression of mucin-2 and occludin	2136:2184	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	0	85	theme	immune	66:71	arg1	response					73:80	altered immune response	58:80	altered immune response	58:80	Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota.
36440681	10	86	theme	cytokine	1702:1709	arg1	secretion					1725:1733	cytokine and chemokine secretion	1702:1733	cytokine and chemokine secretion	1702:1733	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	13	87	theme	b2KO	2082:2085	arg1	mice					2087:2090	b2KO mice	2082:2090	b2KO mice	2082:2090	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	9	88	theme	clinical	1395:1402	arg1	symptoms					1404:1411	clinical symptoms	1395:1411	clinical symptoms	1395:1411	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	4	89	theme	-induced	477:484	arg1	colitis					486:492	dextran sulfate sodium (DSS)-induced colitis	449:492	dextran sulfate sodium (DSS)-induced colitis	449:492	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	0	90	theme	barrier	94:100	arg1	function					102:109	intestinal barrier function	83:109	intestinal barrier function	83:109	Colitis aggravated by Mrgprb2 knockout is associated with altered immune response, intestinal barrier function and gut microbiota.
36440681	8	91	theme	mas-related	979:989	arg1	MRGPRX2					1022:1028	MRGPRX2	1022:1028	MRGPRX2	1022:1028	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	8	91	theme	mas-related	979:989	arg1	X2					1018:1019	Human mas-related G protein-coupled receptor X2	973:1019	Human mas-related G protein-coupled receptor X2 (MRGPRX2)	973:1029	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	8	92	theme	protein-coupled	993:1007	arg1	MRGPRX2					1022:1028	MRGPRX2	1022:1028	MRGPRX2	1022:1028	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	8	92	theme	protein-coupled	993:1007	arg1	X2					1018:1019	Human mas-related G protein-coupled receptor X2	973:1019	Human mas-related G protein-coupled receptor X2 (MRGPRX2)	973:1029	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	9	93	theme	cell	1427:1430	arg1	infiltration					1432:1443	inflammatory cell infiltration	1414:1443	inflammatory cell infiltration	1414:1443	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	8	94	theme	host	1135:1138	arg1	defence					1140:1146	host defence	1135:1146	host defence against microbial infection	1135:1174	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	6	95	theme	ABSTRACT	666:673	arg1	disease					727:733	a chronic immune-related disease	702:733	a chronic immune-related disease	702:733	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	6	95	theme	ABSTRACT	666:673	arg1	UC					695:696	UC	695:696	UC	695:696	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	6	95	theme	ABSTRACT	666:673	arg1	colitis					686:692	ABSTRACT Ulcerative colitis	666:692	ABSTRACT Ulcerative colitis (UC)	666:697	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	2	96	theme	immune	350:355	arg1	response					357:364	immune response	350:364	immune response	350:364	What is the role of mas-related G protein-coupled receptor X2 (MRGPRX2/Mrgprb2) in ulcerative colitis in relation to the intestinal flora, intestinal barrier and immune response?
36440681	11	97	theme	intestinal	1835:1844	arg1	microbiota					1846:1855	the intestinal microbiota	1831:1855	the intestinal microbiota	1831:1855	In addition, the abundance and diversity of the intestinal microbiota were reduced in b2KO mice.
36440681	4	98	theme	gut	528:530	arg1	microbiota					532:541	altered gut microbiota	520:541	altered gut microbiota	520:541	Knockout of mouse Mrgprb2 aggravates dextran sulfate sodium (DSS)-induced colitis, which is associated with altered gut microbiota and immune response and disruption of the intestinal barrier.
36440681	10	99	theme	inflammatory	1740:1751	arg1	infiltration					1758:1769	inflammatory cell infiltration	1740:1769	inflammatory cell infiltration	1740:1769	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	9	100	theme	Mrgprb2	1263:1269	arg1	mice					1292:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	Mrgprb2 knockout (b2KO) male mice	1263:1295	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	101	theme	barrier	1467:1473	arg1	damage					1475:1480	intestinal barrier damage	1456:1480	intestinal barrier damage	1456:1480	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	8	102	theme	mouse	1039:1043	arg1	Mrgprb2					1056:1062	Mrgprb2	1056:1062	Mrgprb2	1056:1062	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	8	102	theme	mouse	1039:1043	arg1	homologue					1045:1053	its mouse homologue	1035:1053	its mouse homologue	1035:1053	Human mas-related G protein-coupled receptor X2 (MRGPRX2) and its mouse homologue, Mrgprb2, are selectively expressed on MCs to recruit immune cells and modulate host defence against microbial infection.
36440681	6	103	theme	chronic	704:710	arg1	colitis					686:692	ABSTRACT Ulcerative colitis	666:692	ABSTRACT Ulcerative colitis (UC)	666:697	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	6	103	theme	chronic	704:710	arg1	disease					727:733	a chronic immune-related disease	702:733	a chronic immune-related disease	702:733	ABSTRACT Ulcerative colitis (UC) is a chronic immune-related disease, and changes in the intestinal microbiota and damage to the intestinal barrier contribute to its pathogenesis.
36440681	10	104	theme	index	1577:1581	arg1	score					1583:1587	disease activity index score	1560:1587	disease activity index score	1560:1587	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	11	105	theme	microbiota	1846:1855	arg1	abundance					1804:1812	abundance	1804:1812	abundance	1804:1812	In addition, the abundance and diversity of the intestinal microbiota were reduced in b2KO mice.
36440681	11	105	theme	microbiota	1846:1855	arg1	diversity					1818:1826	diversity	1818:1826	diversity	1818:1826	In addition, the abundance and diversity of the intestinal microbiota were reduced in b2KO mice.
36440681	10	106	theme	disease	1560:1566	arg1	score					1583:1587	disease activity index score	1560:1587	disease activity index score	1560:1587	The results showed that weight loss, disease activity index score, colon shortening and colonic pathological damage were significantly increased in b2KO mice while MC activation, cytokine and chemokine secretion, and inflammatory cell infiltration were decreased.
36440681	13	107	theme	mice	2087:2090	arg1	damage					2072:2077	Intestinal mucosal barrier damage	2045:2077	Intestinal mucosal barrier damage of b2KO mice	2045:2090	Intestinal mucosal barrier damage of b2KO mice was more severe than that of WT mice due to the attenuated expression of mucin-2 and occludin.
36440681	9	108	from	role	1196:1199	arg1	UC					1215:1216	UC	1215:1216	UC in mice	1215:1224	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	9	109	theme	intestinal	1505:1514	arg1	flora					1516:1520	the intestinal flora	1501:1520	the intestinal flora	1501:1520	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36440681	5	110	contain	have	617:620	arg2	effect					635:640	a protective effect	622:640	a protective effect	622:640	MRGPRB2 may have a protective effect on DSS-induced colitis.
36440681	5	110	contain	have	617:620	arg1	MRGPRB2					605:611	MRGPRB2	605:611	MRGPRB2	605:611	MRGPRB2 may have a protective effect on DSS-induced colitis.
36440681	7	111	theme	Mast	846:849	arg1	MCs					858:860	MCs	858:860	MCs	858:860	Mast cells (MCs) are widely distributed in the gastrointestinal tract and are thought to be related to the pathogenesis of UC.
36440681	7	111	theme	Mast	846:849	arg1	cells					851:855	Mast cells	846:855	Mast cells (MCs)	846:861	Mast cells (MCs) are widely distributed in the gastrointestinal tract and are thought to be related to the pathogenesis of UC.
36440681	9	112	theme	wild-type	1301:1309	arg1	mice					1321:1324	wild-type (WT) male mice	1301:1324	wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis	1301:1374	To investigate the role of Mrgprb2 in UC in mice, we compared the differences between Mrgprb2 knockout (b2KO) male mice and wild-type (WT) male mice with dextran sulfate sodium (DSS)-induced colitis in the severity of clinical symptoms, inflammatory cell infiltration, degree of intestinal barrier damage and composition of the intestinal flora.
36857113	0	0	theme	Dextran	105:111	arg1	Sulfate					113:119	Dextran Sulfate	105:119	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	1	1	theme	main	189:192	arg1	products					204:211	the main cytotoxic products	185:211	the main cytotoxic products of deep-frying oil	185:230	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	1	1	theme	main	189:192	arg1	monomers					172:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	7	2	theme	epithelial	1073:1082	arg1	cells					1084:1088	the intestinal epithelial cells	1058:1088	the intestinal epithelial cells	1058:1088	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway were released through the pores, thus inducing the disintegration of the tight junction between the intestinal epithelial cells and enhancing intestinal permeability.
36857113	1	3	theme	cytotoxic	194:202	arg1	products					204:211	the main cytotoxic products	185:211	the main cytotoxic products of deep-frying oil	185:230	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	1	3	theme	cytotoxic	194:202	arg1	monomers					172:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	7	4	theme	proinflammatory	885:899	arg1	cytokines					901:909	The proinflammatory cytokines	881:909	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway	881:960	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway were released through the pores, thus inducing the disintegration of the tight junction between the intestinal epithelial cells and enhancing intestinal permeability.
36857113	0	5	theme	Sodium-Induced	121:134	arg1	Mice					144:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	4	6	theme	2,3-dioleic	511:521	arg1	EGT					529:531	EGT	529:531	EGT	529:531	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	6	theme	2,3-dioleic	511:521	arg1	acid					523:526	2,3-dioleic acid	511:526	2,3-dioleic acid (EGT)	511:532	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	3	7	theme	main	396:399	arg1	product					411:417	the main oxidation product	392:417	the main oxidation product	392:417	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	3	7	theme	main	396:399	arg1	group					383:387	the epoxy group	373:387	the epoxy group	373:387	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	0	8	theme	Sulfate	113:119	arg1	Mice					144:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	4	9	theme	colitis	672:678	arg1	barrier					630:636	the intestinal barrier	615:636	the intestinal barrier of dextran sulfate sodium-induced colitis	615:678	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	8	10	theme	cell	1313:1316	arg1	membrane					1318:1325	the intestinal epithelial cell membrane	1287:1325	the intestinal epithelial cell membrane	1287:1325	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	2	11	from	impact	246:251	arg1	barrier					271:277	the intestinal barrier	256:277	the intestinal barrier	256:277	However, its impact on the intestinal barrier, the first health guardian, remains unknown.
36857113	4	12	theme	intestinal	619:628	arg1	barrier					630:636	the intestinal barrier	615:636	the intestinal barrier of dextran sulfate sodium-induced colitis	615:678	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	0	13	theme	Colitis	136:142	arg1	Mice					144:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	4	14	theme	1-9,10-epoxystearic	484:502	arg1	ester					504:508	1-9,10-epoxystearic ester	484:508	1-9,10-epoxystearic ester	484:508	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	6	15	theme	expression	775:784	arg1	analysis					786:793	The relative mRNA expression analysis	757:793	The relative mRNA expression analysis	757:793	The relative mRNA expression analysis suggested that EGT could activate Caspase-1/NLRP3/GSDMD, thereby inducing pyroptosis.
36857113	8	16	from	composition	1184:1194	arg1	membrane					1237:1244	the cell membrane	1228:1244	the cell membrane	1228:1244	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	2	17	theme	health	290:295	arg1	impact					246:251	its impact	242:251	its impact on the intestinal barrier	242:277	However, its impact on the intestinal barrier, the first health guardian, remains unknown.
36857113	2	17	theme	health	290:295	arg1	guardian					297:304	the first health guardian	280:304	the first health guardian	280:304	However, its impact on the intestinal barrier, the first health guardian, remains unknown.
36857113	9	18	theme	intestinal	1382:1391	arg1	dysfunction					1393:1403	intestinal dysfunction	1382:1403	intestinal dysfunction	1382:1403	In conclusion, this study highlights that EGT induced intestinal dysfunction via Caspase-1/NLRP3/GSDMD and cGAS-STING pathways.
36857113	8	19	dep	composition	1184:1194	arg1	the					1180:1182	the	1180:1182	the	1180:1182	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	5	20	theme	intestinal	738:747	arg1	damage					749:754	intestinal damage	738:754	intestinal damage	738:754	Characteristics analysis showed that EGT could aggravate intestinal damage.
36857113	1	21	theme	deep-frying	216:226	arg1	oil					228:230	deep-frying oil	216:230	deep-frying oil	216:230	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	0	22	theme	Epoxy	0:4	arg1	Triglyceride					6:17	Epoxy Triglyceride	0:17	Epoxy Triglyceride	0:17	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	6	23	theme	mRNA	770:773	arg1	analysis					786:793	The relative mRNA expression analysis	757:793	The relative mRNA expression analysis	757:793	The relative mRNA expression analysis suggested that EGT could activate Caspase-1/NLRP3/GSDMD, thereby inducing pyroptosis.
36857113	1	24	theme	oil	228:230	arg1	products					204:211	the main cytotoxic products	185:211	the main cytotoxic products of deep-frying oil	185:230	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	1	24	theme	oil	228:230	arg1	monomers					172:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	0	25	theme	Intestinal	28:37	arg1	Permeability					39:50	Intestinal Permeability	28:50	Intestinal Permeability	28:50	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	4	26	theme	glycerol	538:545	arg1	trioleate					547:555	glycerol trioleate	538:555	glycerol trioleate (GT)	538:560	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	26	theme	glycerol	538:545	arg1	GT					558:559	GT	558:559	GT	558:559	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	7	27	theme	intestinal	1104:1113	arg1	permeability					1115:1126	intestinal permeability	1104:1126	intestinal permeability	1104:1126	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway were released through the pores, thus inducing the disintegration of the tight junction between the intestinal epithelial cells and enhancing intestinal permeability.
36857113	6	28	theme	relative	761:768	arg1	analysis					786:793	The relative mRNA expression analysis	757:793	The relative mRNA expression analysis	757:793	The relative mRNA expression analysis suggested that EGT could activate Caspase-1/NLRP3/GSDMD, thereby inducing pyroptosis.
36857113	3	29	theme	cytotoxic	455:463	arg1	it					436:437	it	436:437	it	436:437	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	3	29	theme	cytotoxic	455:463	arg1	factor					465:470	the main cytotoxic factor	446:470	the main cytotoxic factor	446:470	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	9	30	theme	Caspase-1/NLRP3/GSDMD	1409:1429	arg1	pathways					1446:1453	Caspase-1/NLRP3/GSDMD and cGAS-STING pathways	1409:1453	Caspase-1/NLRP3/GSDMD and cGAS-STING pathways	1409:1453	In conclusion, this study highlights that EGT induced intestinal dysfunction via Caspase-1/NLRP3/GSDMD and cGAS-STING pathways.
36857113	5	31	theme	Characteristics	681:695	arg1	analysis					697:704	Characteristics analysis	681:704	Characteristics analysis	681:704	Characteristics analysis showed that EGT could aggravate intestinal damage.
36857113	8	32	from	content	1200:1206	arg1	membrane					1237:1244	the cell membrane	1228:1244	the cell membrane	1228:1244	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	4	33	theme	sodium-induced	657:670	arg1	colitis					672:678	dextran sulfate sodium-induced colitis	641:678	dextran sulfate sodium-induced colitis	641:678	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	8	34	theme	membrane	1318:1325	arg1	changes					1276:1282	the morphological changes	1258:1282	the morphological changes of the intestinal epithelial cell membrane	1258:1325	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	8	35	theme	morphological	1262:1274	arg1	changes					1276:1282	the morphological changes	1258:1282	the morphological changes of the intestinal epithelial cell membrane	1258:1325	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	2	36	theme	intestinal	260:269	arg1	barrier					271:277	the intestinal barrier	256:277	the intestinal barrier	256:277	However, its impact on the intestinal barrier, the first health guardian, remains unknown.
36857113	8	37	theme	epithelial	1302:1311	arg1	membrane					1318:1325	the intestinal epithelial cell membrane	1287:1325	the intestinal epithelial cell membrane	1287:1325	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	8	38	theme	intestinal	1291:1300	arg1	membrane					1318:1325	the intestinal epithelial cell membrane	1287:1325	the intestinal epithelial cell membrane	1287:1325	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	9	39	theme	cGAS-STING	1435:1444	arg1	pathways					1446:1453	Caspase-1/NLRP3/GSDMD and cGAS-STING pathways	1409:1453	Caspase-1/NLRP3/GSDMD and cGAS-STING pathways	1409:1453	In conclusion, this study highlights that EGT induced intestinal dysfunction via Caspase-1/NLRP3/GSDMD and cGAS-STING pathways.
36857113	0	40	from	Pathways	93:100	arg1	Mice					144:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Dextran Sulfate Sodium-Induced Colitis Mice	105:147	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	7	41	theme	junction	1041:1048	arg1	disintegration					1013:1026	the disintegration	1009:1026	the disintegration of the tight junction between the intestinal epithelial cells	1009:1088	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway were released through the pores, thus inducing the disintegration of the tight junction between the intestinal epithelial cells and enhancing intestinal permeability.
36857113	3	42	theme	main	450:453	arg1	it					436:437	it	436:437	it	436:437	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	3	42	theme	main	450:453	arg1	factor					465:470	the main cytotoxic factor	446:470	the main cytotoxic factor	446:470	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	3	43	theme	oxidation	401:409	arg1	product					411:417	the main oxidation product	392:417	the main oxidation product	392:417	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	3	43	theme	oxidation	401:409	arg1	group					383:387	the epoxy group	373:387	the epoxy group	373:387	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	8	44	theme	phospholipids	1211:1223	arg1	content					1200:1206	content	1200:1206	content	1200:1206	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	8	44	theme	phospholipids	1211:1223	arg1	composition					1184:1194	composition	1184:1194	composition	1184:1194	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	4	45	used	used	567:570	arg2	acid					523:526	2,3-dioleic acid	511:526	2,3-dioleic acid (EGT)	511:532	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	45	used	used	567:570	arg2	GT					558:559	GT	558:559	GT	558:559	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	45	used	used	567:570	arg2	trioleate					547:555	glycerol trioleate	538:555	glycerol trioleate (GT)	538:560	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	45	used	used	567:570	arg2	ester					504:508	1-9,10-epoxystearic ester	484:508	1-9,10-epoxystearic ester	484:508	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	45	used	used	567:570	arg2	EGT					529:531	EGT	529:531	EGT	529:531	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	46	from	effect	586:591	arg1	barrier					630:636	the intestinal barrier	615:636	the intestinal barrier of dextran sulfate sodium-induced colitis	615:678	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	0	47	theme	Caspase-1/NLRP3/GSDMD	56:76	arg1	Pathways					93:100	Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways	56:100	Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice	56:147	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	1	48	theme	Oxidized	150:157	arg1	products					204:211	the main cytotoxic products	185:211	the main cytotoxic products of deep-frying oil	185:230	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	1	48	theme	Oxidized	150:157	arg1	monomers					172:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	2	49	theme	first	284:288	arg1	impact					246:251	its impact	242:251	its impact on the intestinal barrier	242:277	However, its impact on the intestinal barrier, the first health guardian, remains unknown.
36857113	2	49	theme	first	284:288	arg1	guardian					297:304	the first health guardian	280:304	the first health guardian	280:304	However, its impact on the intestinal barrier, the first health guardian, remains unknown.
36857113	7	50	theme	tight	1035:1039	arg1	junction					1041:1048	the tight junction	1031:1048	the tight junction	1031:1048	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway were released through the pores, thus inducing the disintegration of the tight junction between the intestinal epithelial cells and enhancing intestinal permeability.
36857113	1	51	theme	triglyceride	159:170	arg1	products					204:211	the main cytotoxic products	185:211	the main cytotoxic products of deep-frying oil	185:230	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	1	51	theme	triglyceride	159:170	arg1	monomers					172:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers	150:179	Oxidized triglyceride monomers are the main cytotoxic products of deep-frying oil.
36857113	7	52	theme	cGAS-STING	943:952	arg1	pathway					954:960	the cGAS-STING pathway	939:960	the cGAS-STING pathway	939:960	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway were released through the pores, thus inducing the disintegration of the tight junction between the intestinal epithelial cells and enhancing intestinal permeability.
36857113	7	53	theme	intestinal	1062:1071	arg1	cells					1084:1088	the intestinal epithelial cells	1058:1088	the intestinal epithelial cells	1058:1088	The proinflammatory cytokines activated by pyroptosis and the cGAS-STING pathway were released through the pores, thus inducing the disintegration of the tight junction between the intestinal epithelial cells and enhancing intestinal permeability.
36857113	8	54	theme	cell	1232:1235	arg1	membrane					1237:1244	the cell membrane	1228:1244	the cell membrane	1228:1244	Metabonomics further confirmed that EGT can change the composition and content of phospholipids on the cell membrane, indicating the morphological changes of the intestinal epithelial cell membrane.
36857113	3	55	theme	HPLC-MS/MS	339:348	arg1	analysis					350:357	HPLC-MS/MS analysis	339:357	HPLC-MS/MS analysis	339:357	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	4	56	theme	group	606:610	arg1	effect					586:591	the effect	582:591	the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis	582:678	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	57	theme	epoxy	600:604	arg1	group					606:610	the epoxy group	596:610	the epoxy group	596:610	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	4	58	theme	sulfate	649:655	arg1	colitis					672:678	dextran sulfate sodium-induced colitis	641:678	dextran sulfate sodium-induced colitis	641:678	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	0	59	theme	cGAS-STING	82:91	arg1	Pathways					93:100	Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways	56:100	Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice	56:147	Epoxy Triglyceride Enhances Intestinal Permeability via Caspase-1/NLRP3/GSDMD and cGAS-STING Pathways in Dextran Sulfate Sodium-Induced Colitis Mice.
36857113	4	60	theme	dextran	641:647	arg1	sulfate					649:655	dextran sulfate	641:655	dextran sulfate sodium-induced colitis	641:678	Therefore, 1-9,10-epoxystearic ester, 2,3-dioleic acid (EGT) and glycerol trioleate (GT) were used to reveal the effect of the epoxy group on the intestinal barrier of dextran sulfate sodium-induced colitis.
36857113	3	61	theme	epoxy	377:381	arg1	product					411:417	the main oxidation product	392:417	the main oxidation product	392:417	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36857113	3	61	theme	epoxy	377:381	arg1	group					383:387	the epoxy group	373:387	the epoxy group	373:387	In this study, HPLC-MS/MS analysis revealed that the epoxy group is the main oxidation product, indicating that it may be the main cytotoxic factor.
36030029	16	0	theme	UC	2879:2880	arg1	mice					2882:2885	UC mice	2879:2885	UC mice in vivo	2879:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	1	1	from	colitis	71:77	arg1	mice					82:85	mice	82:85	mice	82:85	alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota.
36030029	9	2	from	colons	1505:1510	arg1	proportions					1463:1473	The proportions	1459:1473	The proportions of T helper (Th) cells in the colons	1459:1510	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	10	3	theme	Gut	1547:1549	arg1	diversity					1562:1570	Gut microbiota diversity	1547:1570	Gut microbiota diversity	1547:1570	Gut microbiota diversity was detected using 16S ribosomal (r)DNA sequencing.
36030029	2	4	theme	RELEVANCE	193:201	arg1	Licorice					203:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice	172:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice	172:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	16	5	theme	in	2887:2888	arg1	mice					2882:2885	UC mice	2879:2885	UC mice in vivo	2879:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	3	6	theme	licorice	580:587	arg1	effects					569:575	the anti-inflammatory and immunomodulatory effects	526:575	the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway	526:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	15	7	theme	transduction	2586:2597	arg1	pathway					2599:2605	the TLR4/MyD88/NF-κB signal transduction pathway	2558:2605	the TLR4/MyD88/NF-κB signal transduction pathway	2558:2605	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	3	8	dep	composition	700:710	arg1	the					696:698	the	696:698	the	696:698	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	12	9	from	expression	1959:1968	arg1	colons					2008:2013	the colons	2004:2013	the colons	2004:2013	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	12	10	theme	barrier	2066:2072	arg1	integrity					2030:2038	the integrity	2026:2038	the integrity of the intestinal mucosal barrier	2026:2072	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	3	11	theme	intestinal	733:742	arg1	flora					744:748	the intestinal flora	729:748	the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway	729:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	14	12	theme	gut	2296:2298	arg1	microbiota					2300:2309	the gut microbiota	2292:2309	the gut microbiota	2292:2309	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	3	13	theme	effects	569:575	arg1	mechanism					513:521	the mechanism	509:521	the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway	509:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	15	14	theme	TLR4/MyD88/NF-κB	2562:2577	arg1	pathway					2599:2605	the TLR4/MyD88/NF-κB signal transduction pathway	2558:2605	the TLR4/MyD88/NF-κB signal transduction pathway	2558:2605	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	12	15	theme	TLR4/MyD88/NF-κB	1922:1937	arg1	expression					1908:1917	the protein expression	1896:1917	the protein expression of TLR4/MyD88/NF-κB	1896:1937	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	4	16	theme	sulfate	875:881	arg1	DSS					891:893	DSS	891:893	DSS	891:893	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	4	16	theme	sulfate	875:881	arg1	sodium					883:888	dextran sulfate sodium	867:888	dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC)	867:948	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	6	17	theme	Hematoxylin-eosin	1145:1161	arg1	staining					1168:1175	Hematoxylin-eosin (HE) staining	1145:1175	Hematoxylin-eosin (HE) staining	1145:1175	Hematoxylin-eosin (HE) staining was used to evaluate pathological conditions.
36030029	3	18	theme	kappa	777:781	arg1	NF-κB					786:790	NF-κB	786:790	NF-κB	786:790	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	3	18	theme	kappa	777:781	arg1	B					783:783	the nuclear factor kappa B	758:783	the nuclear factor kappa B (NF-κB) signaling pathway	758:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	3	19	theme	domination	602:611	arg1	balance					684:690	the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance	598:690	the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance	598:690	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	16	20	from	bacteria	2757:2764	arg1	colon					2773:2777	the colon	2769:2777	the colon	2769:2777	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	16	20	from	bacteria	2757:2764	arg1	mechanism					2801:2809	a potential mechanism	2789:2809	a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2789:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	4	21	theme	ulcerative	926:935	arg1	UC					946:947	UC	946:947	UC	946:947	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	4	21	theme	ulcerative	926:935	arg1	colitis					937:943	ulcerative colitis	926:943	ulcerative colitis (UC)	926:948	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	16	22	theme	potential	2791:2799	arg1	colon					2773:2777	the colon	2769:2777	the colon	2769:2777	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	16	22	theme	potential	2791:2799	arg1	mechanism					2801:2809	a potential mechanism	2789:2809	a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2789:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	3	23	theme	nuclear	762:768	arg1	NF-κB					786:790	NF-κB	786:790	NF-κB	786:790	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	3	23	theme	nuclear	762:768	arg1	B					783:783	the nuclear factor kappa B	758:783	the nuclear factor kappa B (NF-κB) signaling pathway	758:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	15	24	theme	mucosal	2538:2544	arg1	barrier					2546:2552	the intestinal mucosal barrier	2523:2552	the intestinal mucosal barrier	2523:2552	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	16	25	theme	microbiota	2716:2725	arg1	diversity					2692:2700	the diversity	2688:2700	the diversity of intestinal microbiota	2688:2725	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	16	25	theme	microbiota	2716:2725	arg1	content					2735:2741	the content	2731:2741	the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2731:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	12	26	theme	intestinal	2047:2056	arg1	barrier					2066:2072	the intestinal mucosal barrier	2043:2072	the intestinal mucosal barrier	2043:2072	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	11	27	theme	standard	1745:1752	arg1	protocol					1754:1761	a standard protocol	1743:1761	a standard protocol	1743:1761	In addition, Western blot (WB) assays were used to verify ROR-γt, Foxp3, TLR4, MyD88 and NF-κB expression according to a standard protocol.
36030029	3	28	theme	helper	622:627	arg1	cells					650:654	the T helper type 17/regulatory T cells	616:654	the T helper type 17/regulatory T cells	616:654	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	9	29	theme	T	1478:1478	arg1	Th					1488:1489	Th	1488:1489	Th	1488:1489	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	9	29	theme	T	1478:1478	arg1	helper					1480:1485	T helper	1478:1485	T helper (Th) cells in the colons	1478:1510	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	10	30	theme	16S	1591:1593	arg1	sequencing					1612:1621	16S ribosomal (r)DNA sequencing	1591:1621	16S ribosomal (r)DNA sequencing	1591:1621	Gut microbiota diversity was detected using 16S ribosomal (r)DNA sequencing.
36030029	13	31	theme	UC	2216:2217	arg1	colons					2225:2230	UC mouse colons	2216:2230	UC mouse colons	2216:2230	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	3	32	dep	study	491:495	arg1	AIM					470:472	AIM	470:472	AIM OF THE STUDY	470:485	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	16	33	from	content	2735:2741	arg1	colon					2773:2777	the colon	2769:2777	the colon	2769:2777	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	16	33	from	content	2735:2741	arg1	mechanism					2801:2809	a potential mechanism	2789:2809	a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2789:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	5	34	theme	water	1049:1053	arg1	LWE					1067:1069	LWE	1067:1069	LWE	1067:1069	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	5	34	theme	water	1049:1053	arg1	extraction					1055:1064	licorice water extraction	1040:1064	licorice water extraction (LWE)	1040:1070	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	3	35	theme	cells	650:654	arg1	Th17/Treg					657:665	Th17/Treg	657:665	Th17/Treg	657:665	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	3	35	theme	cells	650:654	arg1	domination					602:611	the domination	598:611	the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance	598:690	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	1	36	theme	intestinal	149:158	arg1	microbiota					160:169	intestinal microbiota	149:169	intestinal microbiota	149:169	alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota.
36030029	16	37	theme	bacteria	2757:2764	arg1	diversity					2692:2700	the diversity	2688:2700	the diversity of intestinal microbiota	2688:2725	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	16	37	theme	bacteria	2757:2764	arg1	content					2735:2741	the content	2731:2741	the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2731:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	12	38	theme	protein	1993:1999	arg1	expression					1959:1968	the expression	1955:1968	the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo	1955:2080	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	3	39	theme	17/regulatory	634:646	arg1	cells					650:654	the T helper type 17/regulatory T cells	616:654	the T helper type 17/regulatory T cells	616:654	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	13	40	theme	ROR-γt	2196:2201	arg1	expression					2182:2191	the protein expression	2170:2191	the protein expression of ROR-γt and Foxp3 in UC mouse colons	2170:2230	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	15	41	theme	Th-cell	2634:2640	arg1	differentiation					2642:2656	Th-cell differentiation	2634:2656	Th-cell differentiation	2634:2656	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	13	42	theme	Foxp3	2207:2211	arg1	expression					2182:2191	the protein expression	2170:2191	the protein expression of ROR-γt and Foxp3 in UC mouse colons	2170:2230	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	12	43	from	inflammation	1842:1853	arg1	tissues					1870:1876	the colonic tissues	1858:1876	the colonic tissues	1858:1876	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	12	44	dep	colons	2008:2013	arg1	improving					2016:2024	improving	2016:2024	improving the integrity of the intestinal mucosal barrier in vivo	2016:2080	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	5	45	theme	pharmacodynamic	959:973	arg1	study					975:979	the pharmacodynamic study	955:979	the pharmacodynamic study	955:979	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	8	46	link	enzyme-linked	1411:1423	arg1	ELISA					1446:1450	ELISA	1446:1450	ELISA	1446:1450	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	8	46	link	enzyme-linked	1411:1423	arg1	assay					1439:1443	enzyme-linked immunosorbent assay	1411:1443	enzyme-linked immunosorbent assay (ELISA) kits	1411:1456	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	14	47	dep	type	2404:2407	arg1	the					2400:2402	the	2400:2402	the	2400:2402	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	11	48	theme	NF-κB	1713:1717	arg1	expression					1719:1728	NF-κB expression	1713:1728	NF-κB expression	1713:1728	In addition, Western blot (WB) assays were used to verify ROR-γt, Foxp3, TLR4, MyD88 and NF-κB expression according to a standard protocol.
36030029	15	49	theme	CONCLUSION	2437:2446	arg1	Licorice					2448:2455	CONCLUSION Licorice	2437:2455	CONCLUSION Licorice	2437:2455	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	9	50	theme	flow	1531:1534	arg1	cytometry					1536:1544	flow cytometry	1531:1544	flow cytometry	1531:1544	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	7	51	theme	intestinal	1323:1332	arg1	barrier					1334:1340	the intestinal barrier	1319:1340	the intestinal barrier of the colons	1319:1354	Immunohistochemistry (IHC) and transmission electron microscopy (TEM) were performed to observe the intestinal barrier of the colons.
36030029	8	52	theme	Inflammatory	1357:1368	arg1	levels					1379:1384	Inflammatory cytokine levels	1357:1384	Inflammatory cytokine levels	1357:1384	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	11	53	theme	Western	1637:1643	arg1	WB					1651:1652	WB	1651:1652	WB	1651:1652	In addition, Western blot (WB) assays were used to verify ROR-γt, Foxp3, TLR4, MyD88 and NF-κB expression according to a standard protocol.
36030029	11	53	theme	Western	1637:1643	arg1	blot					1645:1648	Western blot	1637:1648	Western blot (WB) assays	1637:1660	In addition, Western blot (WB) assays were used to verify ROR-γt, Foxp3, TLR4, MyD88 and NF-κB expression according to a standard protocol.
36030029	10	54	theme	DNA	1608:1610	arg1	sequencing					1612:1621	16S ribosomal (r)DNA sequencing	1591:1621	16S ribosomal (r)DNA sequencing	1591:1621	Gut microbiota diversity was detected using 16S ribosomal (r)DNA sequencing.
36030029	13	55	theme	protein	2174:2180	arg1	expression					2182:2191	the protein expression	2170:2191	the protein expression of ROR-γt and Foxp3 in UC mouse colons	2170:2230	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	1	56	theme	signaling	115:123	arg1	pathways					125:132	NF-κB signaling pathways	109:132	NF-κB signaling pathways	109:132	alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota.
36030029	3	57	theme	differentiation	668:682	arg1	balance					684:690	the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance	598:690	the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance	598:690	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	4	58	theme	BALB/c	834:839	arg1	mice					841:844	MATERIALS AND METHODS BALB/c mice	812:844	MATERIALS AND METHODS BALB/c mice	812:844	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	5	59	theme	colon	1131:1135	arg1	length					1137:1142	colon length	1131:1142	colon length	1131:1142	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	12	60	theme	anti-inflammatory	1802:1818	arg1	effect					1820:1825	a pharmacological anti-inflammatory effect	1784:1825	a pharmacological anti-inflammatory effect	1784:1825	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	13	61	theme	Th17/Treg	2123:2131	arg1	differentiation					2139:2153	Th17/Treg cells differentiation	2123:2153	Th17/Treg cells differentiation	2123:2153	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	13	62	from	imbalance	2110:2118	arg1	differentiation					2139:2153	Th17/Treg cells differentiation	2123:2153	Th17/Treg cells differentiation	2123:2153	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	14	63	theme	species	2363:2369	arg1	composition					2339:2349	the composition	2335:2349	the composition of dominant species	2335:2369	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	2	64	theme	compound	269:276	arg1	compatibility					278:290	compound compatibility	269:290	compound compatibility	269:290	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	2	65	theme	Chinese	242:248	arg1	TCM					260:262	TCM	260:262	TCM	260:262	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	2	65	theme	Chinese	242:248	arg1	Medicine					250:257	traditional Chinese Medicine	230:257	traditional Chinese Medicine (TCM)	230:263	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	8	66	theme	immunosorbent	1425:1437	arg1	ELISA					1446:1450	ELISA	1446:1450	ELISA	1446:1450	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	8	66	theme	immunosorbent	1425:1437	arg1	assay					1439:1443	enzyme-linked immunosorbent assay	1411:1443	enzyme-linked immunosorbent assay (ELISA) kits	1411:1456	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	2	67	theme	medicine	361:368	arg1	efficacy					334:341	efficacy	334:341	efficacy of certain herbal medicine	334:368	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	6	68	used	used	1181:1184	arg2	staining					1168:1175	Hematoxylin-eosin (HE) staining	1145:1175	Hematoxylin-eosin (HE) staining	1145:1175	Hematoxylin-eosin (HE) staining was used to evaluate pathological conditions.
36030029	1	69	theme	dextran	40:46	arg1	sulfate					48:54	dextran sulfate	40:54	dextran sulfate sodium-induced colitis in mice	40:85	alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota.
36030029	7	70	theme	electron	1267:1274	arg1	TEM					1288:1290	TEM	1288:1290	TEM	1288:1290	Immunohistochemistry (IHC) and transmission electron microscopy (TEM) were performed to observe the intestinal barrier of the colons.
36030029	7	70	theme	electron	1267:1274	arg1	microscopy					1276:1285	transmission electron microscopy	1254:1285	transmission electron microscopy (TEM)	1254:1291	Immunohistochemistry (IHC) and transmission electron microscopy (TEM) were performed to observe the intestinal barrier of the colons.
36030029	3	71	theme	anti-inflammatory	530:546	arg1	effects					569:575	the anti-inflammatory and immunomodulatory effects	526:575	the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway	526:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	12	72	theme	tight	1973:1977	arg1	junction					1979:1986	tight junction	1973:1986	tight junction (TJ) protein	1973:1999	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	12	72	theme	tight	1973:1977	arg1	TJ					1989:1990	TJ	1989:1990	TJ	1989:1990	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	1	73	theme	sodium-induced	56:69	arg1	colitis					71:77	dextran sulfate sodium-induced colitis	40:77	dextran sulfate sodium-induced colitis in mice	40:85	alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota.
36030029	13	74	theme	cells	2133:2137	arg1	differentiation					2139:2153	Th17/Treg cells differentiation	2123:2153	Th17/Treg cells differentiation	2123:2153	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	2	75	theme	active	379:384	arg1	components					386:395	its active components	375:395	its active components	375:395	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	15	76	theme	protective	2502:2511	arg1	action					2513:2518	the protective action	2498:2518	the protective action of the intestinal mucosal barrier	2498:2552	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	10	77	theme	microbiota	1551:1560	arg1	diversity					1562:1570	Gut microbiota diversity	1547:1570	Gut microbiota diversity	1547:1570	Gut microbiota diversity was detected using 16S ribosomal (r)DNA sequencing.
36030029	14	78	theme	UC	2314:2315	arg1	mice					2317:2320	UC mice	2314:2320	UC mice	2314:2320	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	9	79	from	cells	1492:1496	arg1	colons					1505:1510	the colons	1501:1510	the colons	1501:1510	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	2	80	theme	ETHNOPHARMACOLOGICAL	172:191	arg1	Licorice					203:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice	172:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice	172:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	14	81	theme	microbiota	2300:2309	arg1	diversity					2279:2287	the diversity	2275:2287	the diversity of the gut microbiota in UC mice	2275:2320	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	3	82	theme	immunomodulatory	552:567	arg1	effects					569:575	the anti-inflammatory and immunomodulatory effects	526:575	the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway	526:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	11	83	used	used	1667:1670	arg2	assays					1655:1660	Western blot (WB) assays	1637:1660	Western blot (WB) assays	1637:1660	In addition, Western blot (WB) assays were used to verify ROR-γt, Foxp3, TLR4, MyD88 and NF-κB expression according to a standard protocol.
36030029	12	84	theme	mucosal	2058:2064	arg1	barrier					2066:2072	the intestinal mucosal barrier	2043:2072	the intestinal mucosal barrier	2043:2072	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	3	85	theme	flora	744:748	arg1	structure					716:724	structure	716:724	structure	716:724	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	3	85	theme	flora	744:748	arg1	balance					684:690	the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance	598:690	the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance	598:690	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	3	85	theme	flora	744:748	arg1	composition					700:710	composition	700:710	composition	700:710	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	2	86	theme	certain	346:352	arg1	medicine					361:368	certain herbal medicine	346:368	certain herbal medicine	346:368	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	15	87	theme	signal	2579:2584	arg1	pathway					2599:2605	the TLR4/MyD88/NF-κB signal transduction pathway	2558:2605	the TLR4/MyD88/NF-κB signal transduction pathway	2558:2605	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	4	88	theme	dextran	867:873	arg1	DSS					891:893	DSS	891:893	DSS	891:893	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	4	88	theme	dextran	867:873	arg1	sodium					883:888	dextran sulfate sodium	867:888	dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC)	867:948	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	14	89	from	diversity	2279:2287	arg1	mice					2317:2320	UC mice	2314:2320	UC mice	2314:2320	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	3	90	theme	B	783:783	arg1	pathway					803:809	the nuclear factor kappa B (NF-κB) signaling pathway	758:809	the nuclear factor kappa B (NF-κB) signaling pathway	758:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	10	91	theme	r	1606:1606	arg1	sequencing					1612:1621	16S ribosomal (r)DNA sequencing	1591:1621	16S ribosomal (r)DNA sequencing	1591:1621	Gut microbiota diversity was detected using 16S ribosomal (r)DNA sequencing.
36030029	3	92	theme	factor	770:775	arg1	NF-κB					786:790	NF-κB	786:790	NF-κB	786:790	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	3	92	theme	factor	770:775	arg1	B					783:783	the nuclear factor kappa B	758:783	the nuclear factor kappa B (NF-κB) signaling pathway	758:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	16	93	theme	anti-inflammatory	2829:2845	arg1	effects					2868:2874	anti-inflammatory and immunomodulatory effects	2829:2874	anti-inflammatory and immunomodulatory effects	2829:2874	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	3	94	theme	T	620:620	arg1	cells					650:654	the T helper type 17/regulatory T cells	616:654	the T helper type 17/regulatory T cells	616:654	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	15	95	theme	barrier	2546:2552	arg1	action					2513:2518	the protective action	2498:2518	the protective action of the intestinal mucosal barrier	2498:2552	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	4	96	theme	colitis	937:943	arg1	models					916:921	animal models	909:921	animal models of ulcerative colitis (UC)	909:948	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	16	97	theme	intestinal	2705:2714	arg1	microbiota					2716:2725	intestinal microbiota	2705:2725	intestinal microbiota	2705:2725	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	10	98	theme	ribosomal	1595:1603	arg1	sequencing					1612:1621	16S ribosomal (r)DNA sequencing	1591:1621	16S ribosomal (r)DNA sequencing	1591:1621	Gut microbiota diversity was detected using 16S ribosomal (r)DNA sequencing.
36030029	3	99	theme	signaling	793:801	arg1	pathway					803:809	the nuclear factor kappa B (NF-κB) signaling pathway	758:809	the nuclear factor kappa B (NF-κB) signaling pathway	758:809	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	16	100	theme	immunomodulatory	2851:2866	arg1	effects					2868:2874	anti-inflammatory and immunomodulatory effects	2829:2874	anti-inflammatory and immunomodulatory effects	2829:2874	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	9	101	theme	helper	1480:1485	arg1	cells					1492:1496	T helper (Th) cells	1478:1496	T helper (Th) cells in the colons	1478:1510	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	12	102	theme	protein	1900:1906	arg1	expression					1908:1917	the protein expression	1896:1917	the protein expression of TLR4/MyD88/NF-κB	1896:1937	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	6	103	theme	pathological	1198:1209	arg1	conditions					1211:1220	pathological conditions	1198:1220	pathological conditions	1198:1220	Hematoxylin-eosin (HE) staining was used to evaluate pathological conditions.
36030029	5	104	theme	licorice	1040:1047	arg1	LWE					1067:1069	LWE	1067:1069	LWE	1067:1069	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	5	104	theme	licorice	1040:1047	arg1	extraction					1055:1064	licorice water extraction	1040:1064	licorice water extraction (LWE)	1040:1070	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	3	105	theme	T	648:648	arg1	cells					650:654	the T helper type 17/regulatory T cells	616:654	the T helper type 17/regulatory T cells	616:654	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	13	106	theme	mouse	2219:2223	arg1	colons					2225:2230	UC mouse colons	2216:2230	UC mouse colons	2216:2230	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	4	107	theme	animal	909:914	arg1	models					916:921	animal models	909:921	animal models of ulcerative colitis (UC)	909:948	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	7	108	theme	colons	1349:1354	arg1	barrier					1334:1340	the intestinal barrier	1319:1340	the intestinal barrier of the colons	1319:1354	Immunohistochemistry (IHC) and transmission electron microscopy (TEM) were performed to observe the intestinal barrier of the colons.
36030029	15	109	theme	differentiation	2642:2656	arg1	imbalance					2621:2629	the imbalance	2617:2629	the imbalance of Th-cell differentiation	2617:2656	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	5	110	theme	extraction	1055:1064	arg1	effect					1030:1035	the anti-inflammatory effect	1008:1035	the anti-inflammatory effect of licorice water extraction (LWE)	1008:1070	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	3	111	theme	type	629:632	arg1	cells					650:654	the T helper type 17/regulatory T cells	616:654	the T helper type 17/regulatory T cells	616:654	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	16	112	from	diversity	2692:2700	arg1	colon					2773:2777	the colon	2769:2777	the colon	2769:2777	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	16	112	from	diversity	2692:2700	arg1	mechanism					2801:2809	a potential mechanism	2789:2809	a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2789:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	9	113	theme	cells	1492:1496	arg1	proportions					1463:1473	The proportions	1459:1473	The proportions of T helper (Th) cells in the colons	1459:1510	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	15	114	theme	intestinal	2527:2536	arg1	barrier					2546:2552	the intestinal mucosal barrier	2523:2552	the intestinal mucosal barrier	2523:2552	CONCLUSION Licorice tends to reduce inflammation and enhance the protective action of the intestinal mucosal barrier via the TLR4/MyD88/NF-κB signal transduction pathway and alter the imbalance of Th-cell differentiation.
36030029	16	115	theme	beneficial	2746:2755	arg1	bacteria					2757:2764	beneficial bacteria	2746:2764	beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2746:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	12	116	theme	colonic	1862:1868	arg1	tissues					1870:1876	the colonic tissues	1858:1876	the colonic tissues	1858:1876	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	16	117	from	colon	2773:2777	arg1	diversity					2692:2700	the diversity	2688:2700	the diversity of intestinal microbiota	2688:2725	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	16	117	from	colon	2773:2777	arg1	content					2735:2741	the content	2731:2741	the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo	2731:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	2	118	used	used	222:225	arg2	Licorice					203:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice	172:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice	172:210	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	13	119	from	expression	2182:2191	arg1	colons					2225:2230	UC mouse colons	2216:2230	UC mouse colons	2216:2230	Moreover, LWE reversed the imbalance in Th17/Treg cells differentiation and influenced the protein expression of ROR-γt and Foxp3 in UC mouse colons.
36030029	2	120	theme	inhibit	420:426	arg1	effects					409:415	effects	409:415	effects of inhibit of inflammation	409:442	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	5	121	theme	clinical	1091:1098	arg1	observation					1100:1110	clinical observation	1091:1110	clinical observation	1091:1110	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	12	122	theme	pharmacological	1786:1800	arg1	effect					1820:1825	a pharmacological anti-inflammatory effect	1784:1825	a pharmacological anti-inflammatory effect	1784:1825	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	1	123	theme	NF-κB	109:113	arg1	pathways					125:132	NF-κB signaling pathways	109:132	NF-κB signaling pathways	109:132	alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota.
36030029	8	124	theme	cytokine	1370:1377	arg1	levels					1379:1384	Inflammatory cytokine levels	1357:1384	Inflammatory cytokine levels	1357:1384	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	11	125	theme	blot	1645:1648	arg1	assays					1655:1660	Western blot (WB) assays	1637:1660	Western blot (WB) assays	1637:1660	In addition, Western blot (WB) assays were used to verify ROR-γt, Foxp3, TLR4, MyD88 and NF-κB expression according to a standard protocol.
36030029	4	126	theme	METHODS	826:832	arg1	mice					841:844	MATERIALS AND METHODS BALB/c mice	812:844	MATERIALS AND METHODS BALB/c mice	812:844	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	3	127	theme	STUDY	481:485	arg1	AIM					470:472	AIM	470:472	AIM OF THE STUDY	470:485	AIM OF THE STUDY The study probed into the mechanism of the anti-inflammatory and immunomodulatory effects of licorice based on the domination of the T helper type 17/regulatory T cells (Th17/Treg) differentiation balance and the composition and structure of the intestinal flora through the nuclear factor kappa B (NF-κB) signaling pathway.
36030029	5	128	theme	length	1137:1142	arg1	measurement					1116:1126	measurement	1116:1126	measurement of colon length	1116:1142	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	5	128	theme	length	1137:1142	arg1	observation					1100:1110	clinical observation	1091:1110	clinical observation	1091:1110	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	5	129	theme	anti-inflammatory	1012:1028	arg1	effect					1030:1035	the anti-inflammatory effect	1008:1035	the anti-inflammatory effect of licorice water extraction (LWE)	1008:1070	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	16	130	dep	in	2887:2888	arg1	vivo					2890:2893	vivo	2890:2893	vivo	2890:2893	Notably, licorice may affect the diversity of intestinal microbiota and the content of beneficial bacteria in the colon, which is a potential mechanism for understanding anti-inflammatory and immunomodulatory effects in UC mice in vivo.
36030029	14	131	theme	dominant	2354:2361	arg1	species					2363:2369	dominant species	2354:2369	dominant species	2354:2369	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	4	132	theme	MATERIALS	812:820	arg1	mice					841:844	MATERIALS AND METHODS BALB/c mice	812:844	MATERIALS AND METHODS BALB/c mice	812:844	MATERIALS AND METHODS BALB/c mice were inoculated with dextran sulfate sodium (DSS) to establish animal models of ulcerative colitis (UC).
36030029	5	133	theme	UC	982:983	arg1	mice					985:988	UC mice	982:988	UC mice	982:988	For the pharmacodynamic study, UC mice were observed for the anti-inflammatory effect of licorice water extraction (LWE) in vivo, including clinical observation and measurement of colon length.
36030029	9	134	from	proportions	1463:1473	arg1	colons					1505:1510	the colons	1501:1510	the colons	1501:1510	The proportions of T helper (Th) cells in the colons was assessed using flow cytometry.
36030029	7	135	theme	transmission	1254:1265	arg1	TEM					1288:1290	TEM	1288:1290	TEM	1288:1290	Immunohistochemistry (IHC) and transmission electron microscopy (TEM) were performed to observe the intestinal barrier of the colons.
36030029	7	135	theme	transmission	1254:1265	arg1	microscopy					1276:1285	transmission electron microscopy	1254:1285	transmission electron microscopy (TEM)	1254:1291	Immunohistochemistry (IHC) and transmission electron microscopy (TEM) were performed to observe the intestinal barrier of the colons.
36030029	2	136	theme	traditional	230:240	arg1	TCM					260:262	TCM	260:262	TCM	260:262	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	2	136	theme	traditional	230:240	arg1	Medicine					250:257	traditional Chinese Medicine	230:257	traditional Chinese Medicine (TCM)	230:263	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	8	137	theme	enzyme-linked	1411:1423	arg1	ELISA					1446:1450	ELISA	1446:1450	ELISA	1446:1450	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	8	137	theme	enzyme-linked	1411:1423	arg1	assay					1439:1443	enzyme-linked immunosorbent assay	1411:1443	enzyme-linked immunosorbent assay (ELISA) kits	1411:1456	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
36030029	2	138	theme	herbal	354:359	arg1	medicine					361:368	certain herbal medicine	346:368	certain herbal medicine	346:368	ETHNOPHARMACOLOGICAL RELEVANCE Licorice is widely used in traditional Chinese Medicine (TCM) for compound compatibility, which could reduce toxicity and increase efficacy of certain herbal medicine, and its active components prominently effects of inhibit of inflammation and regulate of immunity.
36030029	12	139	theme	junction	1979:1986	arg1	protein					1993:1999	tight junction (TJ) protein	1973:1999	tight junction (TJ) protein	1973:1999	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	14	140	theme	probiotics	2425:2434	arg1	quantity					2413:2420	quantity	2413:2420	quantity	2413:2420	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	14	140	theme	probiotics	2425:2434	arg1	type					2404:2407	type	2404:2407	type	2404:2407	In particular, LWE significantly affected the diversity of the gut microbiota in UC mice, ameliorated the composition of dominant species, and significantly increased the type and quantity of probiotics.
36030029	1	141	theme	sulfate	48:54	arg1	colitis					71:77	dextran sulfate sodium-induced colitis	40:77	dextran sulfate sodium-induced colitis in mice	40:85	alleviates dextran sulfate sodium-induced colitis in mice through inhibiting of NF-κB signaling pathways and modulating intestinal microbiota.
36030029	12	142	dep	LWE	1772:1774	arg1	exerted					1776:1782	exerted	1776:1782	LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo	1772:2080	RESULTS LWE exerted a pharmacological anti-inflammatory effect by attenuating inflammation in the colonic tissues through affecting the protein expression of TLR4/MyD88/NF-κB, and increasing the expression of tight junction (TJ) protein in the colons, improving the integrity of the intestinal mucosal barrier in vivo.
36030029	8	143	theme	assay	1439:1443	arg1	kits					1453:1456	enzyme-linked immunosorbent assay (ELISA) kits	1411:1456	enzyme-linked immunosorbent assay (ELISA) kits	1411:1456	Inflammatory cytokine levels were measured using with enzyme-linked immunosorbent assay (ELISA) kits.
37331452	16	0	theme	p65	2359:2361	arg1	translocation					2336:2348	the nuclear translocation	2324:2348	the nuclear translocation of NF-κB p65 and p-STAT3 (Tyr705)	2324:2382	In addition, C1 also reduced the nuclear translocation of NF-κB p65 and p-STAT3 (Tyr705).
37331452	17	1	theme	p-STAT3	2584:2590	arg1	levels					2574:2579	the expression levels	2559:2579	the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32)	2559:2629	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	1	theme	p-STAT3	2584:2590	arg1	expression					2436:2445	the protein expression	2424:2445	the protein expression of α7nAchR	2424:2456	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	1	theme	p-STAT3	2584:2590	arg1	knockdown					2467:2475	the knockdown	2463:2475	the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α	2463:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	18	2	theme	IκBα	2811:2814	arg1	level					2772:2776	the mRNA level	2763:2776	the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish	2763:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	17	3	theme	protein	2428:2434	arg1	expression					2436:2445	the protein expression	2424:2445	the protein expression of α7nAchR	2424:2456	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	10	4	dep	cytokines	1394:1402	arg1	cytokines					1394:1402	The key inflammatory cytokines	1373:1402	The key inflammatory cytokines IL-6 and TNF-α	1373:1417	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	4	dep	cytokines	1394:1402	arg1	TNF-α					1413:1417	TNF-α	1413:1417	TNF-α	1413:1417	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	4	dep	cytokines	1394:1402	arg1	IL-6					1404:1407	IL-6	1404:1407	IL-6	1404:1407	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	15	5	theme	p-IκBα	2245:2250	arg1	level					2236:2240	the expression level	2221:2240	the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells	2221:2292	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	16	6	theme	p-STAT3	2367:2373	arg1	translocation					2336:2348	the nuclear translocation	2324:2348	the nuclear translocation of NF-κB p65 and p-STAT3 (Tyr705)	2324:2382	In addition, C1 also reduced the nuclear translocation of NF-κB p65 and p-STAT3 (Tyr705).
37331452	12	7	theme	immunofluorescence	1695:1712	arg1	assays					1714:1719	immunofluorescence assays	1695:1719	immunofluorescence assays	1695:1719	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65 was evaluated by immunofluorescence assays.
37331452	4	8	theme	water	594:598	arg1	extract					600:606	the water extract	590:606	the water extract of S. miltiorrhiza	590:625	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	11	9	theme	p-STAT3	1519:1525	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	7	10	dep	METHODS	1008:1014	arg1	structures					1020:1029	The structures	1016:1029	METHODS The structures of isolated salvianolic acids	1008:1059	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	11	11	theme	p65	1543:1545	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	17	12	theme	p65	2608:2610	arg1	levels					2574:2579	the expression levels	2559:2579	the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32)	2559:2629	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	12	theme	p65	2608:2610	arg1	expression					2436:2445	the protein expression	2424:2445	the protein expression of α7nAchR	2424:2456	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	12	theme	p65	2608:2610	arg1	knockdown					2467:2475	the knockdown	2463:2475	the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α	2463:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	12	13	theme	p65	1674:1676	arg1	translocation					1630:1642	The nuclear translocation	1618:1642	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65	1618:1676	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65 was evaluated by immunofluorescence assays.
37331452	8	14	theme	zebrafish	1221:1229	arg1	models					1244:1249	the zebrafish inflammation models	1217:1249	the zebrafish inflammation models	1217:1249	Then anti-inflammatory activities of isolates were screened out by the zebrafish inflammation models.
37331452	13	15	theme	LPS-microinjected	1894:1910	arg1	zebrafish					1912:1920	LPS-microinjected zebrafish	1894:1920	LPS-microinjected zebrafish	1894:1920	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	18	16	theme	TNF-α	2787:2791	arg1	level					2772:2776	the mRNA level	2763:2776	the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish	2763:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	15	17	theme	zebrafish	2103:2111	arg1	models					2126:2131	three zebrafish inflammation models	2097:2131	three zebrafish inflammation models	2097:2131	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	17	18	theme	IL-6	2540:2543	arg1	production					2526:2535	the production	2522:2535	the production of IL-6 and TNF-α	2522:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	2	19	theme	CVDs	306:309	arg1	occurrence					292:301	the occurrence	288:301	the occurrence of CVDs	288:309	Inflammation is closely related to the occurrence of CVDs.
37331452	8	20	theme	isolates	1187:1194	arg1	activities					1173:1182	Then anti-inflammatory activities	1150:1182	Then anti-inflammatory activities of isolates	1150:1194	Then anti-inflammatory activities of isolates were screened out by the zebrafish inflammation models.
37331452	17	21	theme	TNF-α	2549:2553	arg1	production					2526:2535	the production	2522:2535	the production of IL-6 and TNF-α	2522:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	13	22	theme	quantitative	1866:1877	arg1	PCR					1879:1881	quantitative PCR	1866:1881	quantitative PCR (Q-PCR)	1866:1889	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	13	22	theme	quantitative	1866:1877	arg1	Q-PCR					1884:1888	Q-PCR	1884:1888	Q-PCR	1884:1888	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	7	23	theme	acids	1055:1059	arg1	structures					1020:1029	The structures	1016:1029	METHODS The structures of isolated salvianolic acids	1008:1059	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	1	24	theme	incidence	223:231	arg1	rate					233:236	a high incidence rate	216:236	a high incidence rate	216:236	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	7	25	theme	isolated	1034:1041	arg1	acids					1055:1059	isolated salvianolic acids	1034:1059	isolated salvianolic acids	1034:1059	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	15	26	theme	RAW	2278:2280	arg1	264.7 cells					2282:2292	LPS stimulated RAW 264.7 cells	2263:2292	LPS stimulated RAW 264.7 cells	2263:2292	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	1	27	theme	ETHNOPHARMACOLOGICAL	130:149	arg1	disease					203:209	a serious disease	193:209	a serious disease with a high incidence rate and mortality	193:250	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	27	theme	ETHNOPHARMACOLOGICAL	130:149	arg1	CVD					185:187	CVD	185:187	CVD	185:187	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	27	theme	ETHNOPHARMACOLOGICAL	130:149	arg1	disease					176:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease	130:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD)	130:188	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	6	28	theme	salvianolic	895:905	arg1	acids					907:911	salvianolic acids	895:911	salvianolic acids from Danshen with anti-inflammatory activity	895:956	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	17	29	theme	C1	2516:2517	arg1	effects					2505:2511	the effects	2501:2511	the effects of C1 on the production of IL-6 and TNF-α	2501:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	11	30	theme	expression	1491:1500	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	15	31	theme	LPS	2263:2265	arg1	264.7 cells					2282:2292	LPS stimulated RAW 264.7 cells	2263:2292	LPS stimulated RAW 264.7 cells	2263:2292	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	1	32	theme	Cardiovascular	161:174	arg1	disease					203:209	a serious disease	193:209	a serious disease with a high incidence rate and mortality	193:250	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	32	theme	Cardiovascular	161:174	arg1	CVD					185:187	CVD	185:187	CVD	185:187	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	32	theme	Cardiovascular	161:174	arg1	disease					176:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease	130:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD)	130:188	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	12	33	theme	NF-κB	1668:1672	arg1	p65					1674:1676	NF-κB p65	1668:1676	NF-κB p65	1668:1676	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65 was evaluated by immunofluorescence assays.
37331452	15	34	theme	best	2149:2152	arg1	activities					2154:2163	the best activities	2145:2163	the best activities	2145:2163	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	10	35	link	enzyme-linked	1436:1448	arg1	ELISA					1471:1475	ELISA	1471:1475	ELISA	1471:1475	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	35	link	enzyme-linked	1436:1448	arg1	assay					1464:1468	enzyme-linked immunosorbent assay	1436:1468	enzyme-linked immunosorbent assay (ELISA)	1436:1476	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	5	36	theme	acids	740:744	arg1	composition					713:723	the complex composition	701:723	the complex composition of salvianolic acids	701:744	However, due to the complex composition of salvianolic acids, the active molecules and their underlying mechanisms have not been fully explored.
37331452	3	37	theme	essential	318:326	arg1	medicine					328:335	an essential medicine	315:335	an essential medicine of promoting blood circulation and removing blood stasis in China	315:401	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	3	37	theme	essential	318:326	arg1	Bunge					424:428	Bunge	424:428	Bunge	424:428	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	10	38	theme	immunosorbent	1450:1462	arg1	ELISA					1471:1475	ELISA	1471:1475	ELISA	1471:1475	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	38	theme	immunosorbent	1450:1462	arg1	assay					1464:1468	enzyme-linked immunosorbent assay	1436:1468	enzyme-linked immunosorbent assay (ELISA)	1436:1476	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	13	39	theme	survival	1844:1851	arg1	analysis					1853:1860	survival analysis	1844:1860	survival analysis	1844:1860	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	18	40	theme	cells	2713:2717	arg1	infiltration					2684:2695	infiltration	2684:2695	infiltration	2684:2695	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	18	40	theme	cells	2713:2717	arg1	migration					2670:2678	migration	2670:2678	migration	2670:2678	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	18	41	theme	IL-6	2781:2784	arg1	level					2772:2776	the mRNA level	2763:2776	the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish	2763:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	6	42	theme	potential	974:982	arg1	mechanisms					984:993	the potential mechanisms	970:993	the potential mechanisms of isolates	970:1005	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	10	43	theme	key	1377:1379	arg1	cytokines					1394:1402	The key inflammatory cytokines	1373:1402	The key inflammatory cytokines IL-6 and TNF-α	1373:1417	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	43	theme	key	1377:1379	arg1	TNF-α					1413:1417	TNF-α	1413:1417	TNF-α	1413:1417	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	43	theme	key	1377:1379	arg1	IL-6					1404:1407	IL-6	1404:1407	IL-6	1404:1407	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	5	44	theme	complex	705:711	arg1	composition					713:723	the complex composition	701:723	the complex composition of salvianolic acids	701:744	However, due to the complex composition of salvianolic acids, the active molecules and their underlying mechanisms have not been fully explored.
37331452	3	45	theme	blood	350:354	arg1	circulation					356:366	blood circulation	350:366	blood circulation	350:366	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	19	46	attach	isolated	2897:2904	arg2	compounds					2882:2890	CONCLUSION Two new and four known compounds	2848:2890	compounds	2882:2890	CONCLUSION Two new and four known compounds were isolated from Danshen.
37331452	19	46	attach	isolated	2897:2904	arg2	new					2863:2865	CONCLUSION Two new and four known compounds	2848:2890	new	2863:2865	CONCLUSION Two new and four known compounds were isolated from Danshen.
37331452	19	46	attach	isolated	2897:2904	arg1	Danshen					2911:2917	Danshen	2911:2917	Danshen	2911:2917	CONCLUSION Two new and four known compounds were isolated from Danshen.
37331452	4	47	from	component	577:585	arg1	extract					600:606	the water extract	590:606	the water extract of S. miltiorrhiza	590:625	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	18	48	theme	survival	2734:2741	arg1	ratio					2743:2747	the survival ratio	2730:2747	the survival ratio	2730:2747	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	5	49	theme	active	751:756	arg1	molecules					758:766	the active molecules	747:766	the active molecules	747:766	However, due to the complex composition of salvianolic acids, the active molecules and their underlying mechanisms have not been fully explored.
37331452	15	50	theme	acid	2019:2022	arg1	C1					2029:2030	C1	2029:2030	C1	2029:2030	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	15	50	theme	acid	2019:2022	arg1	A-1					2024:2026	isosalvianolic acid A-1	2004:2026	isosalvianolic acid A-1 (C1)	2004:2031	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	12	51	theme	nuclear	1622:1628	arg1	translocation					1630:1642	The nuclear translocation	1618:1642	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65	1618:1676	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65 was evaluated by immunofluorescence assays.
37331452	18	52	from	TNF-α	2787:2791	arg1	zebrafish					2837:2845	LPS-microinjected zebrafish	2819:2845	LPS-microinjected zebrafish	2819:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	17	53	theme	p-IκBα	2616:2621	arg1	levels					2574:2579	the expression levels	2559:2579	the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32)	2559:2629	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	53	theme	p-IκBα	2616:2621	arg1	expression					2436:2445	the protein expression	2424:2445	the protein expression of α7nAchR	2424:2456	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	53	theme	p-IκBα	2616:2621	arg1	knockdown					2467:2475	the knockdown	2463:2475	the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α	2463:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	14	54	attach	isolated	1969:1976	arg2	new					1935:1937	RESULTS Two new and four known compounds	1923:1962	new	1935:1937	RESULTS Two new and four known compounds were isolated from Danshen.
37331452	14	54	attach	isolated	1969:1976	arg2	compounds					1954:1962	RESULTS Two new and four known compounds	1923:1962	compounds	1954:1962	RESULTS Two new and four known compounds were isolated from Danshen.
37331452	14	54	attach	isolated	1969:1976	arg1	Danshen					1983:1989	Danshen	1983:1989	Danshen	1983:1989	RESULTS Two new and four known compounds were isolated from Danshen.
37331452	21	55	theme	disease	3214:3220	arg1	treatment					3186:3194	the treatment	3182:3194	the treatment of cardiovascular disease	3182:3220	This study provided evidence for the clinical application of Danshen and contributed to the development of C1 as a novel in the treatment of cardiovascular disease.
37331452	3	56	used	used	450:453	arg2	Bunge					424:428	Bunge	424:428	Bunge	424:428	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	3	56	used	used	450:453	arg2	medicine					328:335	an essential medicine	315:335	an essential medicine of promoting blood circulation and removing blood stasis in China	315:401	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	3	56	used	used	450:453	arg2	Danshen					431:437	Danshen	431:437	Danshen	431:437	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	13	57	theme	staining	1834:1841	arg1	observation					1793:1803	observation	1793:1803	observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish	1793:1920	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	0	58	theme	Salvianolic	0:10	arg1	acids					12:16	Salvianolic acids	0:16	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.	0:128	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.
37331452	14	59	theme	known	1948:1952	arg1	compounds					1954:1962	RESULTS Two new and four known compounds	1923:1962	compounds	1954:1962	RESULTS Two new and four known compounds were isolated from Danshen.
37331452	3	60	theme	blood	381:385	arg1	stasis					387:392	blood stasis	381:392	blood stasis in China	381:401	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	18	61	from	STAT3	2794:2798	arg1	zebrafish					2837:2845	LPS-microinjected zebrafish	2819:2845	LPS-microinjected zebrafish	2819:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	15	62	theme	TNF-α	2201:2205	arg1	secretion					2179:2187	the secretion	2175:2187	the secretion of IL-6 and TNF-α	2175:2205	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	6	63	theme	STUDY	842:846	arg1	AIM					830:832	AIM	830:832	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.	830:1006	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	18	64	from	NF-κB	2801:2805	arg1	zebrafish					2837:2845	LPS-microinjected zebrafish	2819:2845	LPS-microinjected zebrafish	2819:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	13	65	theme	neutrophil	1808:1817	arg1	migration					1819:1827	neutrophil migration	1808:1827	neutrophil migration	1808:1827	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	7	66	theme	dichroism	1120:1128	arg1	UV					1080:1081	UV	1080:1081	UV	1080:1081	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	7	66	theme	dichroism	1120:1128	arg1	calculations					1136:1147	electronic circular dichroism (ECD) calculations	1100:1147	electronic circular dichroism (ECD) calculations	1100:1147	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	9	67	theme	LPS-stimulated	1341:1354	arg1	264.7 cells					1360:1370	LPS-stimulated RAW 264.7 cells	1341:1370	LPS-stimulated RAW 264.7 cells	1341:1370	The most active compound was further used to explore the anti-inflammatory mechanisms on LPS-stimulated RAW 264.7 cells.
37331452	20	68	theme	α7nAchR	2986:2992	arg1	signaling					2994:3002	α7nAchR signaling	2986:3002	α7nAchR signaling	2986:3002	Among them, C1 exerted anti-inflammatory activities by activating α7nAchR signaling and subsequently inhibiting STAT3 and NF-κB pathways.
37331452	17	69	from	effects	2505:2511	arg1	production					2526:2535	the production	2522:2535	the production of IL-6 and TNF-α	2522:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	6	70	dep	AIM	830:832	arg1	aims					866:869	aims	866:869	aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates	866:1005	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	18	71	from	IκBα	2811:2814	arg1	zebrafish					2837:2845	LPS-microinjected zebrafish	2819:2845	LPS-microinjected zebrafish	2819:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	0	72	theme	anti-inflammatory	59:75	arg1	effects					77:83	their anti-inflammatory effects	53:83	their anti-inflammatory effects	53:83	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.
37331452	7	73	theme	electronic	1100:1109	arg1	UV					1080:1081	UV	1080:1081	UV	1080:1081	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	7	73	theme	electronic	1100:1109	arg1	calculations					1136:1147	electronic circular dichroism (ECD) calculations	1100:1147	electronic circular dichroism (ECD) calculations	1100:1147	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	1	74	with	disease	203:209	arg1	mortality					242:250	mortality	242:250	mortality	242:250	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	74	with	disease	203:209	arg1	rate					233:236	a high incidence rate	216:236	a high incidence rate	216:236	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	13	75	from	observation	1793:1803	arg1	zebrafish					1912:1920	LPS-microinjected zebrafish	1894:1920	LPS-microinjected zebrafish	1894:1920	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	4	76	theme	Salvianolic	537:547	arg1	component					577:585	the most abundant component	559:585	the most abundant component	559:585	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	4	76	theme	Salvianolic	537:547	arg1	acids					549:553	Salvianolic acids	537:553	Salvianolic acids	537:553	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	6	77	from	Danshen	918:924	arg1	acids					907:911	salvianolic acids	895:911	salvianolic acids from Danshen with anti-inflammatory activity	895:956	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	18	78	theme	NF-κB	2801:2805	arg1	level					2772:2776	the mRNA level	2763:2776	the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish	2763:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	15	79	theme	ethyl	2037:2041	arg1	C5					2058:2059	C5	2058:2059	C5	2058:2059	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	15	79	theme	ethyl	2037:2041	arg1	lithospermate					2043:2055	ethyl lithospermate	2037:2055	ethyl lithospermate (C5)	2037:2060	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	13	80	theme	anti-inflammatory	1743:1759	arg1	mechanisms					1761:1770	the in vivo anti-inflammatory mechanisms	1731:1770	the in vivo anti-inflammatory mechanisms	1731:1770	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	11	81	theme	α7nAchR	1573:1579	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	3	82	theme	cardiovascular	502:515	arg1	effects					528:534	its anti-inflammatory and cardiovascular protective effects	476:534	its anti-inflammatory and cardiovascular protective effects	476:534	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	19	83	theme	CONCLUSION	2848:2857	arg1	new					2863:2865	CONCLUSION Two new and four known compounds	2848:2890	new	2863:2865	CONCLUSION Two new and four known compounds were isolated from Danshen.
37331452	18	84	theme	STAT3	2794:2798	arg1	level					2772:2776	the mRNA level	2763:2776	the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish	2763:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	18	85	from	level	2772:2776	arg1	zebrafish					2837:2845	LPS-microinjected zebrafish	2819:2845	LPS-microinjected zebrafish	2819:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	11	86	theme	Western	1600:1606	arg1	blotting					1608:1615	Western blotting	1600:1615	Western blotting	1600:1615	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	3	87	theme	anti-inflammatory	480:496	arg1	effects					528:534	its anti-inflammatory and cardiovascular protective effects	476:534	its anti-inflammatory and cardiovascular protective effects	476:534	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	18	88	theme	LPS-microinjected	2819:2835	arg1	zebrafish					2837:2845	LPS-microinjected zebrafish	2819:2845	LPS-microinjected zebrafish	2819:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	18	89	from	IL-6	2781:2784	arg1	zebrafish					2837:2845	LPS-microinjected zebrafish	2819:2845	LPS-microinjected zebrafish	2819:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	10	90	theme	inflammatory	1381:1392	arg1	cytokines					1394:1402	The key inflammatory cytokines	1373:1402	The key inflammatory cytokines IL-6 and TNF-α	1373:1417	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	90	theme	inflammatory	1381:1392	arg1	TNF-α					1413:1417	TNF-α	1413:1417	TNF-α	1413:1417	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	90	theme	inflammatory	1381:1392	arg1	IL-6					1404:1407	IL-6	1404:1407	IL-6	1404:1407	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	15	91	theme	expression	2225:2234	arg1	level					2236:2240	the expression level	2221:2240	the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells	2221:2292	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	13	92	theme	in	1735:1736	arg1	mechanisms					1761:1770	the in vivo anti-inflammatory mechanisms	1731:1770	the in vivo anti-inflammatory mechanisms	1731:1770	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	15	93	theme	inflammation	2113:2124	arg1	models					2126:2131	three zebrafish inflammation models	2097:2131	three zebrafish inflammation models	2097:2131	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	6	94	theme	present	852:858	arg1	study					860:864	The present study	848:864	The present study	848:864	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	17	95	theme	NF-κB	2602:2606	arg1	p65					2608:2610	NF-κB p65	2602:2610	NF-κB p65	2602:2610	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	4	96	theme	significant	640:650	arg1	effect					652:657	a significant effect	638:657	a significant effect	638:657	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	18	97	theme	vivo	2635:2638	arg1	experiments					2640:2650	vivo experiments	2635:2650	vivo experiments	2635:2650	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	1	98	theme	serious	195:201	arg1	disease					203:209	a serious disease	193:209	a serious disease with a high incidence rate and mortality	193:250	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	98	theme	serious	195:201	arg1	disease					176:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease	130:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD)	130:188	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	13	99	theme	PCR	1879:1881	arg1	observation					1793:1803	observation	1793:1803	observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish	1793:1920	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	11	100	theme	NF-κB	1537:1541	arg1	p65					1543:1545	NF-κB p65	1537:1545	NF-κB p65	1537:1545	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	8	101	theme	anti-inflammatory	1155:1171	arg1	activities					1173:1182	Then anti-inflammatory activities	1150:1182	Then anti-inflammatory activities of isolates	1150:1194	Then anti-inflammatory activities of isolates were screened out by the zebrafish inflammation models.
37331452	6	102	theme	anti-inflammatory	931:947	arg1	activity					949:956	anti-inflammatory activity	931:956	anti-inflammatory activity	931:956	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	15	103	from	level	2236:2240	arg1	264.7 cells					2282:2292	LPS stimulated RAW 264.7 cells	2263:2292	LPS stimulated RAW 264.7 cells	2263:2292	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	8	104	theme	inflammation	1231:1242	arg1	models					1244:1249	the zebrafish inflammation models	1217:1249	the zebrafish inflammation models	1217:1249	Then anti-inflammatory activities of isolates were screened out by the zebrafish inflammation models.
37331452	7	105	theme	salvianolic	1043:1053	arg1	acids					1055:1059	isolated salvianolic acids	1034:1059	isolated salvianolic acids	1034:1059	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	18	106	theme	mRNA	2767:2770	arg1	level					2772:2776	the mRNA level	2763:2776	the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish	2763:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	1	107	theme	high	218:221	arg1	rate					233:236	a high incidence rate	216:236	a high incidence rate	216:236	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	15	108	theme	IL-6	2192:2195	arg1	secretion					2179:2187	the secretion	2175:2187	the secretion of IL-6 and TNF-α	2175:2205	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	0	109	from	effects	77:83	arg1	acids					12:16	Salvianolic acids	0:16	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.	0:128	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.
37331452	17	110	theme	expression	2563:2572	arg1	levels					2574:2579	the expression levels	2559:2579	the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32)	2559:2629	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	15	111	with	C1	2137:2138	arg1	activities					2154:2163	the best activities	2145:2163	the best activities	2145:2163	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	11	112	theme	protein	1483:1489	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	1	113	theme	RELEVANCE	151:159	arg1	disease					203:209	a serious disease	193:209	a serious disease with a high incidence rate and mortality	193:250	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	113	theme	RELEVANCE	151:159	arg1	CVD					185:187	CVD	185:187	CVD	185:187	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	1	113	theme	RELEVANCE	151:159	arg1	disease					176:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease	130:182	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD)	130:188	ETHNOPHARMACOLOGICAL RELEVANCE Cardiovascular disease (CVD) is a serious disease with a high incidence rate and mortality.
37331452	5	114	theme	salvianolic	728:738	arg1	acids					740:744	salvianolic acids	728:744	salvianolic acids	728:744	However, due to the complex composition of salvianolic acids, the active molecules and their underlying mechanisms have not been fully explored.
37331452	15	115	theme	stimulated	2267:2276	arg1	264.7 cells					2282:2292	LPS stimulated RAW 264.7 cells	2263:2292	LPS stimulated RAW 264.7 cells	2263:2292	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	6	116	theme	isolates	998:1005	arg1	mechanisms					984:993	the potential mechanisms	970:993	the potential mechanisms of isolates	970:1005	AIM OF THIS STUDY The present study aims to isolate and identify salvianolic acids from Danshen with anti-inflammatory activity and explore the potential mechanisms of isolates.
37331452	11	117	theme	STAT3	1512:1516	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	9	118	theme	active	1261:1266	arg1	compound					1268:1275	The most active compound	1252:1275	The most active compound	1252:1275	The most active compound was further used to explore the anti-inflammatory mechanisms on LPS-stimulated RAW 264.7 cells.
37331452	10	119	theme	enzyme-linked	1436:1448	arg1	ELISA					1471:1475	ELISA	1471:1475	ELISA	1471:1475	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	10	119	theme	enzyme-linked	1436:1448	arg1	assay					1464:1468	enzyme-linked immunosorbent assay	1436:1468	enzyme-linked immunosorbent assay (ELISA)	1436:1476	The key inflammatory cytokines IL-6 and TNF-α were measured by enzyme-linked immunosorbent assay (ELISA).
37331452	17	120	theme	α7nAchR	2450:2456	arg1	levels					2574:2579	the expression levels	2559:2579	the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32)	2559:2629	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	120	theme	α7nAchR	2450:2456	arg1	expression					2436:2445	the protein expression	2424:2445	the protein expression of α7nAchR	2424:2456	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	120	theme	α7nAchR	2450:2456	arg1	knockdown					2467:2475	the knockdown	2463:2475	the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α	2463:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	4	121	theme	CVDs	679:682	arg1	treatment					666:674	the treatment	662:674	the treatment of CVDs	662:682	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	13	122	theme	analysis	1853:1860	arg1	observation					1793:1803	observation	1793:1803	observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish	1793:1920	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	18	123	theme	inflammatory	2700:2711	arg1	cells					2713:2717	inflammatory cells	2700:2717	inflammatory cells	2700:2717	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	9	124	used	used	1289:1292	arg2	compound					1268:1275	The most active compound	1252:1275	The most active compound	1252:1275	The most active compound was further used to explore the anti-inflammatory mechanisms on LPS-stimulated RAW 264.7 cells.
37331452	12	125	theme	p-STAT3	1647:1653	arg1	translocation					1630:1642	The nuclear translocation	1618:1642	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65	1618:1676	The nuclear translocation of p-STAT3 (Tyr705) and NF-κB p65 was evaluated by immunofluorescence assays.
37331452	20	126	theme	anti-inflammatory	2943:2959	arg1	activities					2961:2970	anti-inflammatory activities	2943:2970	anti-inflammatory activities	2943:2970	Among them, C1 exerted anti-inflammatory activities by activating α7nAchR signaling and subsequently inhibiting STAT3 and NF-κB pathways.
37331452	13	127	theme	migration	1819:1827	arg1	observation					1793:1803	observation	1793:1803	observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish	1793:1920	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	21	128	theme	clinical	3095:3102	arg1	application					3104:3114	the clinical application	3091:3114	the clinical application of Danshen	3091:3125	This study provided evidence for the clinical application of Danshen and contributed to the development of C1 as a novel in the treatment of cardiovascular disease.
37331452	0	129	theme	signaling	119:127	arg1	activation					97:106	the activation	93:106	the activation of α7nAchR signaling	93:127	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.
37331452	3	130	from	stasis	387:392	arg1	China					397:401	China	397:401	China	397:401	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	9	131	from	mechanisms	1327:1336	arg1	264.7 cells					1360:1370	LPS-stimulated RAW 264.7 cells	1341:1370	LPS-stimulated RAW 264.7 cells	1341:1370	The most active compound was further used to explore the anti-inflammatory mechanisms on LPS-stimulated RAW 264.7 cells.
37331452	15	132	theme	isosalvianolic	2004:2017	arg1	C1					2029:2030	C1	2029:2030	C1	2029:2030	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	15	132	theme	isosalvianolic	2004:2017	arg1	A-1					2024:2026	isosalvianolic acid A-1	2004:2026	isosalvianolic acid A-1 (C1)	2004:2031	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	18	133	dep	migration	2670:2678	arg1	the					2666:2668	the	2666:2668	the	2666:2668	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	0	134	from	Bunge	43:47	arg1	acids					12:16	Salvianolic acids	0:16	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.	0:128	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.
37331452	21	135	theme	cardiovascular	3199:3212	arg1	disease					3214:3220	cardiovascular disease	3199:3220	cardiovascular disease	3199:3220	This study provided evidence for the clinical application of Danshen and contributed to the development of C1 as a novel in the treatment of cardiovascular disease.
37331452	13	136	theme	H&E	1830:1832	arg1	staining					1834:1841	H&E staining	1830:1841	H&E staining	1830:1841	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	5	137	theme	underlying	778:787	arg1	mechanisms					789:798	their underlying mechanisms	772:798	their underlying mechanisms	772:798	However, due to the complex composition of salvianolic acids, the active molecules and their underlying mechanisms have not been fully explored.
37331452	21	138	from	treatment	3186:3194	arg1	novel					3173:3177	novel	3173:3177	novel	3173:3177	This study provided evidence for the clinical application of Danshen and contributed to the development of C1 as a novel in the treatment of cardiovascular disease.
37331452	17	139	theme	α7nAchR	2480:2486	arg1	levels					2574:2579	the expression levels	2559:2579	the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32)	2559:2629	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	139	theme	α7nAchR	2480:2486	arg1	expression					2436:2445	the protein expression	2424:2445	the protein expression of α7nAchR	2424:2456	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	17	139	theme	α7nAchR	2480:2486	arg1	knockdown					2467:2475	the knockdown	2463:2475	the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α	2463:2553	Moreover, C1 significantly upregulated the protein expression of α7nAchR, and the knockdown of α7nAchR counteracted the effects of C1 on the production of IL-6 and TNF-α and the expression levels of p-STAT3 (Tyr705), NF-κB p65 and p-IκBα (Ser32).
37331452	9	140	theme	anti-inflammatory	1309:1325	arg1	mechanisms					1327:1336	the anti-inflammatory mechanisms	1305:1336	the anti-inflammatory mechanisms on LPS-stimulated RAW 264.7 cells	1305:1370	The most active compound was further used to explore the anti-inflammatory mechanisms on LPS-stimulated RAW 264.7 cells.
37331452	16	141	theme	nuclear	2328:2334	arg1	translocation					2336:2348	the nuclear translocation	2324:2348	the nuclear translocation of NF-κB p65 and p-STAT3 (Tyr705)	2324:2382	In addition, C1 also reduced the nuclear translocation of NF-κB p65 and p-STAT3 (Tyr705).
37331452	4	142	theme	abundant	568:575	arg1	component					577:585	the most abundant component	559:585	the most abundant component	559:585	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	4	142	theme	abundant	568:575	arg1	acids					549:553	Salvianolic acids	537:553	Salvianolic acids	537:553	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	4	143	contain	has	634:636	arg1	component					577:585	the most abundant component	559:585	the most abundant component	559:585	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	4	143	contain	has	634:636	arg1	acids					549:553	Salvianolic acids	537:553	Salvianolic acids	537:553	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	4	143	contain	has	634:636	arg2	effect					652:657	a significant effect	638:657	a significant effect	638:657	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	7	144	theme	circular	1111:1118	arg1	ECD					1131:1133	ECD	1131:1133	ECD	1131:1133	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	7	144	theme	circular	1111:1118	arg1	dichroism					1120:1128	circular dichroism	1111:1128	electronic circular dichroism (ECD) calculations	1100:1147	METHODS The structures of isolated salvianolic acids were elucidated by UV, IR, NMR, MS and electronic circular dichroism (ECD) calculations.
37331452	21	145	theme	C1	3165:3166	arg1	development					3150:3160	the development	3146:3160	the development of C1 as a novel in the treatment of cardiovascular disease	3146:3220	This study provided evidence for the clinical application of Danshen and contributed to the development of C1 as a novel in the treatment of cardiovascular disease.
37331452	18	146	from	zebrafish	2837:2845	arg1	level					2772:2776	the mRNA level	2763:2776	the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish	2763:2845	In vivo experiments, C1 decreased the migration and infiltration of inflammatory cells, increased the survival ratio and inhibited the mRNA level of IL-6, TNF-α, STAT3, NF-κB and IκBα in LPS-microinjected zebrafish.
37331452	11	147	theme	p-IκBα	1554:1559	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	0	148	theme	α7nAchR	111:117	arg1	signaling					119:127	α7nAchR signaling	111:127	α7nAchR signaling	111:127	Salvianolic acids from Salvia miltiorrhiza Bunge and their anti-inflammatory effects through the activation of α7nAchR signaling.
37331452	9	149	theme	RAW	1356:1358	arg1	264.7 cells					1360:1370	LPS-stimulated RAW 264.7 cells	1341:1370	LPS-stimulated RAW 264.7 cells	1341:1370	The most active compound was further used to explore the anti-inflammatory mechanisms on LPS-stimulated RAW 264.7 cells.
37331452	13	150	dep	in	1735:1736	arg1	vivo					1738:1741	vivo	1738:1741	vivo	1738:1741	Finally, the in vivo anti-inflammatory mechanisms were investigated by observation of neutrophil migration, H&E staining, survival analysis and quantitative PCR (Q-PCR) in LPS-microinjected zebrafish.
37331452	20	151	theme	NF-κB	3042:3046	arg1	pathways					3048:3055	NF-κB pathways	3042:3055	NF-κB pathways	3042:3055	Among them, C1 exerted anti-inflammatory activities by activating α7nAchR signaling and subsequently inhibiting STAT3 and NF-κB pathways.
37331452	14	152	theme	RESULTS	1923:1929	arg1	new					1935:1937	RESULTS Two new and four known compounds	1923:1962	new	1935:1937	RESULTS Two new and four known compounds were isolated from Danshen.
37331452	21	153	from	novel	3173:3177	arg1	treatment					3186:3194	the treatment	3182:3194	the treatment of cardiovascular disease	3182:3220	This study provided evidence for the clinical application of Danshen and contributed to the development of C1 as a novel in the treatment of cardiovascular disease.
37331452	19	154	theme	known	2876:2880	arg1	compounds					2882:2890	CONCLUSION Two new and four known compounds	2848:2890	compounds	2882:2890	CONCLUSION Two new and four known compounds were isolated from Danshen.
37331452	11	155	theme	IκBα	1548:1551	arg1	levels					1502:1507	The protein expression levels	1479:1507	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR	1479:1579	The protein expression levels of STAT3, p-STAT3 (Tyr705), NF-κB p65, IκBα, p-IκBα (Ser32) and α7nAchR were determined by Western blotting.
37331452	21	156	theme	Danshen	3119:3125	arg1	application					3104:3114	the clinical application	3091:3114	the clinical application of Danshen	3091:3125	This study provided evidence for the clinical application of Danshen and contributed to the development of C1 as a novel in the treatment of cardiovascular disease.
37331452	3	157	theme	due	469:471	arg1	CVDs					464:467	CVDs	464:467	CVDs due to its anti-inflammatory and cardiovascular protective effects	464:534	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
37331452	15	158	theme	neutrophil	2072:2081	arg1	migrations					2083:2092	neutrophil migrations	2072:2092	neutrophil migrations	2072:2092	Among them, isosalvianolic acid A-1 (C1) and ethyl lithospermate (C5) inhibited neutrophil migrations in three zebrafish inflammation models and C1 with the best activities decreased the secretion of IL-6 and TNF-α and inhibited the expression level of p-IκBα (Ser32) in LPS stimulated RAW 264.7 cells.
37331452	4	159	theme	miltiorrhiza	614:625	arg1	extract					600:606	the water extract	590:606	the water extract of S. miltiorrhiza	590:625	Salvianolic acids are the most abundant component in the water extract of S. miltiorrhiza, which has a significant effect on the treatment of CVDs.
37331452	16	160	theme	NF-κB	2353:2357	arg1	p65					2359:2361	NF-κB p65	2353:2361	NF-κB p65	2353:2361	In addition, C1 also reduced the nuclear translocation of NF-κB p65 and p-STAT3 (Tyr705).
37331452	3	161	theme	protective	517:526	arg1	effects					528:534	its anti-inflammatory and cardiovascular protective effects	476:534	its anti-inflammatory and cardiovascular protective effects	476:534	As an essential medicine of promoting blood circulation and removing blood stasis in China, Salvia miltiorrhiza Bunge (Danshen) is widely used to treat CVDs due to its anti-inflammatory and cardiovascular protective effects.
36173731	8	0	theme	average	1097:1103	arg1	identity					1116:1123	average nucleotide identity	1097:1123	average nucleotide identity between the three strains and their respective closest strains	1097:1186	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	2	1	theme	Legionella-like	100:114	arg1	isolates					116:123	Legionella-like isolates	100:123	Legionella-like isolates	100:123	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	1	theme	Legionella-like	100:114	arg1	30cs62T					153:159	30cs62T	153:159	30cs62T	153:159	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	1	theme	Legionella-like	100:114	arg1	strains					126:132	strains 27fs60	126:139	strains 27fs60	126:139	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	1	theme	Legionella-like	100:114	arg1	30fs61					142:147	30fs61	142:147	30fs61	142:147	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	3	2	theme	B-cycloheximide	494:508	arg1	agar					510:513	glycine-vancomycin-polymyxin B-cycloheximide agar	465:513	glycine-vancomycin-polymyxin B-cycloheximide agar	465:513	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	6	3	theme	similarity	880:889	arg1	values					891:896	similarity values	880:896	similarity values	880:896	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	3	4	with	32-37 °C	372:379	arg1	optimum					390:396	an optimum	387:396	an optimum at 32 °C	387:405	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	3	5	dep	Neelsen-stain-negative	282:303	arg1	rod-shaped					306:315	rod-shaped	306:315	rod-shaped	306:315	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	6	6	theme	gene	770:773	arg1	sequences					775:783	The mip and rpoB gene sequences	753:783	sequences	775:783	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	3	7	theme	medium	532:537	arg1	agar					539:542	Wadowsky-Yee medium agar	519:542	Wadowsky-Yee medium agar	519:542	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	6	8	theme	quateirensis	850:861	arg1	49507T					868:873	Legionella quateirensis ATCC 49507T	839:873	Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively	839:929	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	8	9	dep	%	1200:1200	arg1	12376T					1223:1228	12376T	1223:1228	12376T	1223:1228	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	11	10	theme	independent	1712:1722	arg1	species					1724:1730	an independent species	1709:1730	an independent species	1709:1730	The results obtained confirm the status of an independent species.
36173731	7	11	theme	39.1	1027:1030	arg1	contents					1009:1016	G+C contents	1005:1016	G+C contents of 39.0, 39.1 and 39.0 mol%, respectively	1005:1058	Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively.
36173731	10	12	theme	quateirensis	1640:1651	arg1	ATCC					1653:1656	L. quateirensis ATCC 49507T	1637:1663	L. quateirensis ATCC 49507T	1637:1663	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	8	13	theme	closest	1172:1178	arg1	strains					1180:1186	their respective closest strains	1155:1186	their respective closest strains	1155:1186	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	7	14	theme	39.0	1021:1024	arg1	contents					1009:1016	G+C contents	1005:1016	G+C contents of 39.0, 39.1 and 39.0 mol%, respectively	1005:1058	Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively.
36173731	8	15	dep	%	1277:1277	arg1	49507T					1300:1305	L. quateirensis ATCC 49507T	1279:1305	91.45 % L. quateirensis ATCC 49507T	1271:1305	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	6	16	theme	%	915:915	arg1	values					891:896	similarity values	880:896	similarity values	880:896	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	10	17	theme	16S	1569:1571	arg1	rRNA					1573:1576	16S rRNA	1569:1576	16S rRNA	1569:1576	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	10	17	theme	16S	1569:1571	arg1	genes					1598:1602	rpoB and rnpB genes	1584:1602	genes	1598:1602	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	10	17	theme	16S	1569:1571	arg1	mip					1579:1581	mip	1579:1581	mip	1579:1581	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	2	18	theme	water	189:193	arg1	system					208:213	a hotel water distribution system	181:213	a hotel water distribution system in the Emilia-Romagna region, Italy	181:249	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	7	19	theme	strains	969:975	arg1	sequencing					945:954	Whole genome sequencing	932:954	Whole genome sequencing of the three strains	932:975	Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively.
36173731	8	20	theme	quateirensis	1242:1253	arg1	49507T					1260:1265	L. quateirensis ATCC 49507T	1239:1265	91.45 % L. quateirensis ATCC 49507T	1231:1265	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	9	21	theme	L.	1465:1466	arg1	relatedness					1511:1521	≤40.10 % DNA-DNA relatedness	1494:1521	≤40.10 % DNA-DNA relatedness	1494:1521	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	21	theme	L.	1465:1466	arg1	49507T					1486:1491	L. quateirensis ATCC 49507T	1465:1491	L. quateirensis ATCC 49507T	1465:1491	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	22	theme	phylogenetic	1432:1443	arg1	species					1456:1462	the most related phylogenetic Legionella species	1415:1462	the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness)	1415:1522	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	23	theme	ATCC	1481:1484	arg1	relatedness					1511:1521	≤40.10 % DNA-DNA relatedness	1494:1521	≤40.10 % DNA-DNA relatedness	1494:1521	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	23	theme	ATCC	1481:1484	arg1	49507T					1486:1491	L. quateirensis ATCC 49507T	1465:1491	L. quateirensis ATCC 49507T	1465:1491	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	7	24	theme	Whole	932:936	arg1	sequencing					945:954	Whole genome sequencing	932:954	Whole genome sequencing of the three strains	932:975	Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively.
36173731	6	25	theme	Legionella	839:848	arg1	49507T					868:873	Legionella quateirensis ATCC 49507T	839:873	Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively	839:929	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	8	26	theme	L.	1279:1280	arg1	49507T					1300:1305	L. quateirensis ATCC 49507T	1279:1305	91.45 % L. quateirensis ATCC 49507T	1271:1305	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	13	27	theme	TSD-262T=DSM	1823:1834	arg1	30cs62T					1808:1814	30cs62T	1808:1814	30cs62T (=ATCC TSD-262T=DSM 112526T)	1808:1843	nov. with 30cs62T (=ATCC TSD-262T=DSM 112526T) as the type strain.
36173731	13	27	theme	TSD-262T=DSM	1823:1834	arg1	112526T					1836:1842	=ATCC TSD-262T=DSM 112526T	1817:1842	=ATCC TSD-262T=DSM 112526T	1817:1842	nov. with 30cs62T (=ATCC TSD-262T=DSM 112526T) as the type strain.
36173731	9	28	theme	%	1501:1501	arg1	relatedness					1511:1521	≤40.10 % DNA-DNA relatedness	1494:1521	≤40.10 % DNA-DNA relatedness	1494:1521	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	28	theme	%	1501:1501	arg1	49507T					1486:1491	L. quateirensis ATCC 49507T	1465:1491	L. quateirensis ATCC 49507T	1465:1491	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	3	29	theme	transitory	323:332	arg1	presence					343:350	transitory flagella presence	323:350	transitory flagella presence	323:350	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	8	30	theme	ATCC	1295:1298	arg1	49507T					1300:1305	L. quateirensis ATCC 49507T	1279:1305	91.45 % L. quateirensis ATCC 49507T	1271:1305	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	10	31	theme	rnpB	1593:1596	arg1	rRNA					1573:1576	16S rRNA	1569:1576	16S rRNA	1569:1576	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	10	31	theme	rnpB	1593:1596	arg1	genes					1598:1602	rpoB and rnpB genes	1584:1602	genes	1598:1602	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	1	32	theme	distribution	61:72	arg1	system					74:79	a hotel water distribution system	47:79	a hotel water distribution system in northern Italy	47:97	nov., isolated from a hotel water distribution system in northern Italy.
36173731	10	33	theme	rpoB	1584:1587	arg1	rRNA					1573:1576	16S rRNA	1569:1576	16S rRNA	1569:1576	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	10	33	theme	rpoB	1584:1587	arg1	genes					1598:1602	rpoB and rnpB genes	1584:1602	genes	1598:1602	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	0	34	theme	Legionella	0:9	arg1	sp					23:24	Legionella bononiensis sp	0:24	Legionella bononiensis sp.	0:25	Legionella bononiensis sp.
36173731	7	35	theme	%	1044:1044	arg1	contents					1009:1016	G+C contents	1005:1016	G+C contents of 39.0, 39.1 and 39.0 mol%, respectively	1005:1058	Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively.
36173731	3	36	theme	charcoal-yeast	420:433	arg1	agar					443:446	buffered charcoal-yeast extract agar	411:446	buffered charcoal-yeast extract agar with l-cysteine	411:462	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	10	37	with	relationship	1619:1630	arg1	ATCC					1653:1656	L. quateirensis ATCC 49507T	1637:1663	L. quateirensis ATCC 49507T	1637:1663	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	10	38	theme	L.	1637:1638	arg1	ATCC					1653:1656	L. quateirensis ATCC 49507T	1637:1663	L. quateirensis ATCC 49507T	1637:1663	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	13	39	theme	type	1852:1855	arg1	strain					1857:1862	the type strain	1848:1862	the type strain	1848:1862	nov. with 30cs62T (=ATCC TSD-262T=DSM 112526T) as the type strain.
36173731	12	40	theme	Legionella	1771:1780	arg1	name					1737:1740	The name	1733:1740	The name proposed for this species	1733:1766	The name proposed for this species is Legionella bononiensis sp.
36173731	12	40	theme	Legionella	1771:1780	arg1	sp					1794:1795	Legionella bononiensis sp	1771:1795	Legionella bononiensis sp	1771:1795	The name proposed for this species is Legionella bononiensis sp.
36173731	9	41	theme	DNA-DNA	1334:1340	arg1	hybridization					1342:1354	The digital DNA-DNA hybridization	1322:1354	The digital DNA-DNA hybridization analysis	1322:1363	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	3	42	theme	glycine-vancomycin-polymyxin	465:492	arg1	agar					510:513	glycine-vancomycin-polymyxin B-cycloheximide agar	465:513	glycine-vancomycin-polymyxin B-cycloheximide agar	465:513	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	6	43	with	49507T	868:873	arg1	values					891:896	similarity values	880:896	similarity values	880:896	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	10	44	theme	concatenated	1529:1540	arg1	tree					1555:1558	The concatenated phylogenetic tree	1525:1558	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes,	1525:1603	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	3	45	with	agar	510:513	arg1	l-cysteine					453:462	l-cysteine	453:462	l-cysteine	453:462	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	8	46	theme	nucleotide	1105:1114	arg1	identity					1116:1123	average nucleotide identity	1097:1123	average nucleotide identity between the three strains and their respective closest strains	1097:1186	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	3	47	with	agar	443:446	arg1	l-cysteine					453:462	l-cysteine	453:462	l-cysteine	453:462	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	3	48	theme	Wadowsky-Yee	519:530	arg1	agar					539:542	Wadowsky-Yee medium agar	519:542	Wadowsky-Yee medium agar	519:542	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	6	49	theme	ATCC	863:866	arg1	49507T					868:873	Legionella quateirensis ATCC 49507T	839:873	Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively	839:929	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	6	50	theme	rpoB	765:768	arg1	sequences					775:783	The mip and rpoB gene sequences	753:783	sequences	775:783	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	11	51	theme	species	1724:1730	arg1	status					1699:1704	the status	1695:1704	the status of an independent species	1695:1730	The results obtained confirm the status of an independent species.
36173731	9	52	theme	related	1424:1430	arg1	species					1456:1462	the most related phylogenetic Legionella species	1415:1462	the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness)	1415:1522	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	8	53	theme	respective	1161:1170	arg1	strains					1180:1186	their respective closest strains	1155:1186	their respective closest strains	1155:1186	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	4	54	theme	positive	634:641	arg1	reaction					643:650	a weakly positive reaction	625:650	a weakly positive reaction for catalase	625:663	The strains showed positive reactions for oxidase, hippurate and gelatinase and a weakly positive reaction for catalase.
36173731	7	55	theme	G+C	1005:1007	arg1	contents					1009:1016	G+C contents	1005:1016	G+C contents of 39.0, 39.1 and 39.0 mol%, respectively	1005:1058	Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively.
36173731	2	56	theme	distribution	195:206	arg1	system					208:213	a hotel water distribution system	181:213	a hotel water distribution system in the Emilia-Romagna region, Italy	181:249	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	12	57	theme	bononiensis	1782:1792	arg1	name					1737:1740	The name	1733:1740	The name proposed for this species	1733:1766	The name proposed for this species is Legionella bononiensis sp.
36173731	12	57	theme	bononiensis	1782:1792	arg1	sp					1794:1795	Legionella bononiensis sp	1771:1795	Legionella bononiensis sp	1771:1795	The name proposed for this species is Legionella bononiensis sp.
36173731	9	58	theme	Legionella	1445:1454	arg1	species					1456:1462	the most related phylogenetic Legionella species	1415:1462	the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness)	1415:1522	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	13	59	with	nov.	1798:1801	arg1	30cs62T					1808:1814	30cs62T	1808:1814	30cs62T (=ATCC TSD-262T=DSM 112526T)	1808:1843	nov. with 30cs62T (=ATCC TSD-262T=DSM 112526T) as the type strain.
36173731	13	59	with	nov.	1798:1801	arg1	112526T					1836:1842	=ATCC TSD-262T=DSM 112526T	1817:1842	=ATCC TSD-262T=DSM 112526T	1817:1842	nov. with 30cs62T (=ATCC TSD-262T=DSM 112526T) as the type strain.
36173731	8	60	theme	L.	1239:1240	arg1	49507T					1260:1265	L. quateirensis ATCC 49507T	1239:1265	91.45 % L. quateirensis ATCC 49507T	1231:1265	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	2	61	theme	Emilia-Romagna	222:235	arg1	Italy					245:249	Italy	245:249	Italy	245:249	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	61	theme	Emilia-Romagna	222:235	arg1	region					237:242	the Emilia-Romagna region	218:242	the Emilia-Romagna region	218:242	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	9	62	theme	quateirensis	1468:1479	arg1	relatedness					1511:1521	≤40.10 % DNA-DNA relatedness	1494:1521	≤40.10 % DNA-DNA relatedness	1494:1521	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	62	theme	quateirensis	1468:1479	arg1	49507T					1486:1491	L. quateirensis ATCC 49507T	1465:1491	L. quateirensis ATCC 49507T	1465:1491	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	10	63	theme	phylogenetic	1542:1553	arg1	tree					1555:1558	The concatenated phylogenetic tree	1525:1558	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes,	1525:1603	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	8	64	theme	ATCC	1255:1258	arg1	49507T					1260:1265	L. quateirensis ATCC 49507T	1239:1265	91.45 % L. quateirensis ATCC 49507T	1231:1265	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	5	65	theme	strain	695:700	arg1	30cs62T					702:708	strain 30cs62T	695:708	strain 30cs62T	695:708	Based on the EUCAST cut-off, strain 30cs62T was resistant to ciprofloxacin (5 mg l-1).
36173731	13	66	theme	=ATCC	1817:1821	arg1	30cs62T					1808:1814	30cs62T	1808:1814	30cs62T (=ATCC TSD-262T=DSM 112526T)	1808:1843	nov. with 30cs62T (=ATCC TSD-262T=DSM 112526T) as the type strain.
36173731	13	66	theme	=ATCC	1817:1821	arg1	112526T					1836:1842	=ATCC TSD-262T=DSM 112526T	1817:1842	=ATCC TSD-262T=DSM 112526T	1817:1842	nov. with 30cs62T (=ATCC TSD-262T=DSM 112526T) as the type strain.
36173731	7	67	theme	genome	938:943	arg1	sequencing					945:954	Whole genome sequencing	932:954	Whole genome sequencing of the three strains	932:975	Whole genome sequencing of the three strains was performed, resulting in G+C contents of 39.0, 39.1 and 39.0 mol%, respectively.
36173731	9	68	theme	≤40.10 	1494:1500	arg1	%					1501:1501	%	1501:1501	%	1501:1501	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	1	69	theme	hotel	49:53	arg1	system					74:79	a hotel water distribution system	47:79	a hotel water distribution system in northern Italy	47:97	nov., isolated from a hotel water distribution system in northern Italy.
36173731	9	70	theme	DNA-DNA	1503:1509	arg1	relatedness					1511:1521	≤40.10 % DNA-DNA relatedness	1494:1521	≤40.10 % DNA-DNA relatedness	1494:1521	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	70	theme	DNA-DNA	1503:1509	arg1	49507T					1486:1491	L. quateirensis ATCC 49507T	1465:1491	L. quateirensis ATCC 49507T	1465:1491	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	3	71	with	Gram-	266:270	arg1	able					356:359	able	356:359	able	356:359	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	3	71	with	Gram-	266:270	arg1	presence					343:350	transitory flagella presence	323:350	transitory flagella presence	323:350	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	3	72	theme	flagella	334:341	arg1	presence					343:350	transitory flagella presence	323:350	transitory flagella presence	323:350	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	8	73	theme	quateirensis	1282:1293	arg1	49507T					1300:1305	L. quateirensis ATCC 49507T	1279:1305	91.45 % L. quateirensis ATCC 49507T	1271:1305	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	0	74	theme	bononiensis	11:21	arg1	sp					23:24	Legionella bononiensis sp	0:24	Legionella bononiensis sp.	0:25	Legionella bononiensis sp.
36173731	2	75	from	system	208:213	arg1	Italy					245:249	Italy	245:249	Italy	245:249	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	75	from	system	208:213	arg1	region					237:242	the Emilia-Romagna region	218:242	the Emilia-Romagna region	218:242	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	76	attach	isolated	167:174	arg1	system					208:213	a hotel water distribution system	181:213	a hotel water distribution system in the Emilia-Romagna region, Italy	181:249	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	76	attach	isolated	167:174	arg2	30fs61					142:147	30fs61	142:147	30fs61	142:147	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	76	attach	isolated	167:174	arg2	strains					126:132	strains 27fs60	126:139	strains 27fs60	126:139	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	76	attach	isolated	167:174	arg2	30cs62T					153:159	30cs62T	153:159	30cs62T	153:159	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	2	76	attach	isolated	167:174	arg2	isolates					116:123	Legionella-like isolates	100:123	Legionella-like isolates	100:123	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	1	77	theme	northern	84:91	arg1	Italy					93:97	northern Italy	84:97	northern Italy	84:97	nov., isolated from a hotel water distribution system in northern Italy.
36173731	4	78	theme	positive	564:571	arg1	reactions					573:581	positive reactions	564:581	positive reactions for oxidase, hippurate and gelatinase	564:619	The strains showed positive reactions for oxidase, hippurate and gelatinase and a weakly positive reaction for catalase.
36173731	1	79	theme	water	55:59	arg1	system					74:79	a hotel water distribution system	47:79	a hotel water distribution system in northern Italy	47:97	nov., isolated from a hotel water distribution system in northern Italy.
36173731	3	80	theme	extract	435:441	arg1	agar					443:446	buffered charcoal-yeast extract agar	411:446	buffered charcoal-yeast extract agar with l-cysteine	411:462	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	3	81	theme	buffered	411:418	arg1	agar					443:446	buffered charcoal-yeast extract agar	411:446	buffered charcoal-yeast extract agar with l-cysteine	411:462	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	3	82	with	agar	539:542	arg1	l-cysteine					453:462	l-cysteine	453:462	l-cysteine	453:462	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	2	83	theme	hotel	183:187	arg1	system					208:213	a hotel water distribution system	181:213	a hotel water distribution system in the Emilia-Romagna region, Italy	181:249	Legionella-like isolates, strains 27fs60, 30fs61 and 30cs62T, were isolated from a hotel water distribution system in the Emilia-Romagna region, Italy.
36173731	8	84	theme	identity	1065:1072	arg1	%					1200:1200	91.32 %	1194:1200	91.32 % L. quateirensis NCTC 12376T	1194:1228	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	8	84	theme	identity	1065:1072	arg1	percentage					1074:1083	The identity percentage	1061:1083	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains	1061:1186	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	6	85	theme	close	813:817	arg1	matches					819:825	close matches	813:825	close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively	813:929	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	9	86	dep	species	1456:1462	arg1	relatedness					1511:1521	≤40.10 % DNA-DNA relatedness	1494:1521	≤40.10 % DNA-DNA relatedness	1494:1521	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	9	86	dep	species	1456:1462	arg1	49507T					1486:1491	L. quateirensis ATCC 49507T	1465:1491	L. quateirensis ATCC 49507T	1465:1491	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	10	87	theme	close	1613:1617	arg1	relationship					1619:1630	a close relationship	1611:1630	a close relationship with L. quateirensis ATCC 49507T	1611:1663	The concatenated phylogenetic tree based on 16S rRNA, mip, rpoB and rnpB genes, shows a close relationship with L. quateirensis ATCC 49507T.
36173731	1	88	from	system	74:79	arg1	Italy					93:97	northern Italy	84:97	northern Italy	84:97	nov., isolated from a hotel water distribution system in northern Italy.
36173731	9	89	theme	digital	1326:1332	arg1	hybridization					1342:1354	The digital DNA-DNA hybridization	1322:1354	The digital DNA-DNA hybridization analysis	1322:1363	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36173731	6	90	theme	strains	798:804	arg1	mip					757:759	The mip and rpoB gene sequences	753:783	mip	757:759	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	6	90	theme	strains	798:804	arg1	sequences					775:783	The mip and rpoB gene sequences	753:783	sequences	775:783	The mip and rpoB gene sequences of the three strains showed close matches to those of Legionella quateirensis ATCC 49507T with similarity values of 98.2 and 94.5 %, respectively.
36173731	8	91	dep	%	1237:1237	arg1	49507T					1260:1265	L. quateirensis ATCC 49507T	1239:1265	91.45 % L. quateirensis ATCC 49507T	1231:1265	The identity percentage measured by average nucleotide identity between the three strains and their respective closest strains were: 91.32 % L. quateirensis NCTC 12376T, 91.45 % L. quateirensis ATCC 49507T and 91.45 % L. quateirensis ATCC 49507T, respectively.
36173731	3	92	from	32 °C	401:405	arg1	optimum					390:396	an optimum	387:396	an optimum at 32 °C	387:405	Isolates were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, with transitory flagella presence and able to grow at 32-37 °C (with an optimum at 32 °C) on buffered charcoal-yeast extract agar with l-cysteine, glycine-vancomycin-polymyxin B-cycloheximide agar and Wadowsky-Yee medium agar.
36173731	9	93	theme	hybridization	1342:1354	arg1	analysis					1356:1363	The digital DNA-DNA hybridization analysis	1322:1363	The digital DNA-DNA hybridization analysis	1322:1363	The digital DNA-DNA hybridization analysis demonstrated how the isolates were separated from the most related phylogenetic Legionella species (L. quateirensis ATCC 49507T, ≤40.10 % DNA-DNA relatedness).
36044822	0	0	theme	NHX	84:86	arg1	antiporter					88:97	NHX antiporter	84:97	NHX antiporter	84:97	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	4	1	theme	high-industrial	435:449	arg1	value					451:455	its high-industrial value	431:455	its high-industrial value	431:455	In advance, its need is increasing because of its high-industrial value and population blast.
36044822	5	2	theme	Vitis	706:710	arg1	vinifera					712:719	Vitis vinifera	706:719	Vitis vinifera	706:719	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	1	3	theme	nutritional	135:145	arg1	critical					210:217	critical	210:217	critical	210:217	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	1	3	theme	nutritional	135:145	arg1	L.					195:196	L.	195:196	L.	195:196	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	1	3	theme	nutritional	135:145	arg1	enhancement					147:157	The nutritional enhancement	131:157	The nutritional enhancement of potato plants	131:174	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	7	4	theme	parameters	968:977	arg1	evaluation					941:950	the evaluation	937:950	the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers,	937:1081	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	5	theme	different	1122:1130	arg1	lines					1132:1136	the different lines	1118:1136	the different lines	1118:1136	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	4	6	theme	population	461:470	arg1	blast					472:476	population blast	461:476	population blast	461:476	In advance, its need is increasing because of its high-industrial value and population blast.
36044822	6	7	theme	transgenic	744:753	arg1	plants					755:760	Control and transgenic plants	732:760	Control and transgenic plants	732:760	Control and transgenic plants were grown in greenhouse and field under non-stressed conditions for 85 days in order to characterize their phenotype and evaluate their agronomical performance.
36044822	8	8	theme	starch	1353:1358	arg1	contents					1360:1367	increased protein and starch contents	1331:1367	contents	1360:1367	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	9	9	theme	overexpression	1521:1534	arg1	effect					1505:1510	the effect	1501:1510	the effect of their overexpression in potato plants	1501:1551	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	8	10	dep	growth	1212:1217	arg1	growth					1277:1282	better vegetative growth	1259:1282	better vegetative growth	1259:1282	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	8	10	dep	growth	1212:1217	arg1	precocity					1230:1238	flowering precocity	1220:1238	flowering precocity	1220:1238	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	8	10	dep	growth	1212:1217	arg1	gain					1241:1244	gain	1241:1244	gain of vigor	1241:1253	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	9	11	theme	potato	1539:1544	arg1	plants					1546:1551	potato plants	1539:1551	potato plants	1539:1551	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	2	12	theme	basic	252:256	arg1	nutrition					269:277	a worldwide basic vegetarian nutrition	240:277	a worldwide basic vegetarian nutrition to maintain health	240:296	As it is considered a worldwide basic vegetarian nutrition to maintain health.
36044822	9	13	theme	VvNHX	1433:1437	arg1	antiport					1439:1446	the VvNHX antiport	1429:1446	the VvNHX antiport	1429:1446	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	6	14	theme	Control	732:738	arg1	plants					755:760	Control and transgenic plants	732:760	Control and transgenic plants	732:760	Control and transgenic plants were grown in greenhouse and field under non-stressed conditions for 85 days in order to characterize their phenotype and evaluate their agronomical performance.
36044822	2	15	theme	worldwide	242:250	arg1	nutrition					269:277	a worldwide basic vegetarian nutrition	240:277	a worldwide basic vegetarian nutrition to maintain health	240:296	As it is considered a worldwide basic vegetarian nutrition to maintain health.
36044822	5	16	from	vinifera	664:671	arg1	antiporter					642:651	a sodium proton antiporter	626:651	a sodium proton antiporter from Vitis vinifera	626:671	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	0	17	theme	chloride	104:111	arg1	channel					113:119	CLC chloride channel	100:119	CLC chloride channel	100:119	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	5	18	theme	transgenic	572:581	arg1	plants					590:595	transgenic potato plants	572:595	transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both	572:729	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	8	19	theme	better	1259:1264	arg1	growth					1277:1282	better vegetative growth	1259:1282	better vegetative growth	1259:1282	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	1	20	theme	plants	169:174	arg1	critical					210:217	critical	210:217	critical	210:217	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	1	20	theme	plants	169:174	arg1	L.					195:196	L.	195:196	L.	195:196	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	1	20	theme	plants	169:174	arg1	enhancement					147:157	The nutritional enhancement	131:157	The nutritional enhancement of potato plants	131:174	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	8	21	theme	obtained	1143:1150	arg1	results					1152:1158	The obtained results	1139:1158	The obtained results	1139:1158	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	0	22	theme	CLC	100:102	arg1	channel					113:119	CLC chloride channel	100:119	CLC chloride channel	100:119	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	5	23	theme	potato	583:588	arg1	plants					590:595	transgenic potato plants	572:595	transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both	572:729	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	1	24	theme	potato	162:167	arg1	plants					169:174	potato plants	162:174	potato plants	162:174	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	6	25	theme	non-stressed	803:814	arg1	conditions					816:825	non-stressed conditions	803:825	non-stressed conditions for 85 days	803:837	Control and transgenic plants were grown in greenhouse and field under non-stressed conditions for 85 days in order to characterize their phenotype and evaluate their agronomical performance.
36044822	9	26	from	effect	1505:1510	arg1	plants					1546:1551	potato plants	1539:1551	potato plants	1539:1551	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	3	27	theme	crop	379:382	arg1	one					315:317	one	315:317	one	315:317	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	3	27	theme	crop	379:382	arg1	crop					379:382	the world's fourth-largest food crop	347:382	the world's fourth-largest food crop	347:382	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	3	27	theme	crop	379:382	arg1	staples					335:341	the foremost staples	322:341	the foremost staples	322:341	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	0	28	theme	Improved	0:7	arg1	growth					9:14	Improved growth	0:14	Improved growth	0:14	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	7	29	theme	chemical	1051:1058	arg1	parameters					968:977	plant growth parameters	955:977	plant growth parameters	955:977	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	29	theme	chemical	1051:1058	arg1	composition					1060:1070	the chemical composition	1047:1070	the chemical composition of tubers	1047:1080	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	5	30	theme	harsh	528:532	arg1	conditions					548:557	harsh environmental conditions	528:557	harsh environmental conditions	528:557	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	8	31	theme	vegetative	1266:1275	arg1	growth					1277:1282	better vegetative growth	1259:1282	better vegetative growth	1259:1282	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	3	32	theme	foremost	326:333	arg1	staples					335:341	the foremost staples	322:341	the foremost staples	322:341	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	6	33	theme	agronomical	899:909	arg1	performance					911:921	their agronomical performance	893:921	their agronomical performance	893:921	Control and transgenic plants were grown in greenhouse and field under non-stressed conditions for 85 days in order to characterize their phenotype and evaluate their agronomical performance.
36044822	5	34	theme	Vitis	658:662	arg1	vinifera					664:671	Vitis vinifera	658:671	Vitis vinifera	658:671	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	5	35	theme	environmental	534:546	arg1	conditions					548:557	harsh environmental conditions	528:557	harsh environmental conditions	528:557	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	8	36	theme	transgenic	1172:1181	arg1	plants					1183:1188	transgenic plants	1172:1188	transgenic plants	1172:1188	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	3	37	theme	staples	335:341	arg1	one					315:317	one	315:317	one	315:317	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	3	37	theme	staples	335:341	arg1	crop					379:382	the world's fourth-largest food crop	347:382	the world's fourth-largest food crop	347:382	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	3	37	theme	staples	335:341	arg1	staples					335:341	the foremost staples	322:341	the foremost staples	322:341	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	7	38	theme	plant	955:959	arg1	parameters					968:977	plant growth parameters	955:977	plant growth parameters	955:977	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	38	theme	plant	955:959	arg1	yields					986:991	tuber yields	980:991	tuber yields	980:991	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	38	theme	plant	955:959	arg1	composition					1060:1070	the chemical composition	1047:1070	the chemical composition of tubers	1047:1080	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	38	theme	plant	955:959	arg1	characteristics					997:1011	characteristics	997:1011	characteristics	997:1011	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	2	39	theme	vegetarian	258:267	arg1	nutrition					269:277	a worldwide basic vegetarian nutrition	240:277	a worldwide basic vegetarian nutrition to maintain health	240:296	As it is considered a worldwide basic vegetarian nutrition to maintain health.
36044822	3	40	theme	fourth-largest	359:372	arg1	crop					379:382	the world's fourth-largest food crop	347:382	the world's fourth-largest food crop	347:382	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	7	41	theme	tuber	980:984	arg1	parameters					968:977	plant growth parameters	955:977	plant growth parameters	955:977	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	41	theme	tuber	980:984	arg1	yields					986:991	tuber yields	980:991	tuber yields	980:991	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	41	theme	tuber	980:984	arg1	color					1039:1043	color	1039:1043	color	1039:1043	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	41	theme	tuber	980:984	arg1	calibers					1014:1021	calibers	1014:1021	calibers	1014:1021	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	41	theme	tuber	980:984	arg1	number					1028:1033	eye number	1024:1033	eye number	1024:1033	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	1	42	dep	critical	210:217	arg1	critical					210:217	critical	210:217	critical	210:217	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	1	42	dep	critical	210:217	arg1	L.					195:196	L.	195:196	L.	195:196	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	1	42	dep	critical	210:217	arg1	enhancement					147:157	The nutritional enhancement	131:157	The nutritional enhancement of potato plants	131:174	The nutritional enhancement of potato plants (Solanum tuberosum L.,) is highly critical.
36044822	8	43	theme	enhanced	1296:1303	arg1	yields					1311:1316	enhanced tuber yields	1296:1316	enhanced tuber yields	1296:1316	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	3	44	theme	food	374:377	arg1	crop					379:382	the world's fourth-largest food crop	347:382	the world's fourth-largest food crop	347:382	S. tuberosum is one of the foremost staples and the world's fourth-largest food crop.
36044822	9	45	theme	effect	1505:1510	arg1	insight					1397:1403	insight	1397:1403	insight into the role played by the VvNHX antiport and the VvCLC channel	1397:1468	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	9	45	theme	effect	1505:1510	arg1	understanding					1484:1496	a greater understanding	1474:1496	a greater understanding of the effect of their overexpression in potato plants	1474:1551	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	0	46	theme	tuber	20:24	arg1	quality					26:32	tuber quality	20:32	tuber quality	20:32	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	5	47	theme	chloride	684:691	arg1	channel					693:699	a chloride channel	682:699	a chloride channel from Vitis vinifera	682:719	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	0	48	theme	potato	48:53	arg1	plants					55:60	transgenic potato plants	37:60	transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both	37:128	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	9	49	from	plants	1546:1551	arg1	effect					1505:1510	the effect	1501:1510	the effect of their overexpression in potato plants	1501:1551	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	5	50	theme	sodium	628:633	arg1	antiporter					642:651	a sodium proton antiporter	626:651	a sodium proton antiporter from Vitis vinifera	626:671	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	7	51	dep	yields	986:991	arg1	yields					986:991	tuber yields	980:991	tuber yields	980:991	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	51	dep	yields	986:991	arg1	color					1039:1043	color	1039:1043	color	1039:1043	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	51	dep	yields	986:991	arg1	calibers					1014:1021	calibers	1014:1021	calibers	1014:1021	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	51	dep	yields	986:991	arg1	number					1028:1033	eye number	1024:1033	eye number	1024:1033	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	5	52	theme	potato	495:500	arg1	growth					502:507	potato growth	495:507	potato growth	495:507	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	8	53	theme	improved	1203:1210	arg1	growth					1212:1217	an improved growth	1200:1217	an improved growth (flowering precocity, gain of vigor and better vegetative growth)	1200:1283	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	0	54	theme	transgenic	37:46	arg1	plants					55:60	transgenic potato plants	37:60	transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both	37:128	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	9	55	from	overexpression	1521:1534	arg1	plants					1546:1551	potato plants	1539:1551	potato plants	1539:1551	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	5	56	theme	proton	635:640	arg1	antiporter					642:651	a sodium proton antiporter	626:651	a sodium proton antiporter from Vitis vinifera	626:671	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	7	57	theme	eye	1024:1026	arg1	yields					986:991	tuber yields	980:991	tuber yields	980:991	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	57	theme	eye	1024:1026	arg1	number					1028:1033	eye number	1024:1033	eye number	1024:1033	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	58	theme	tubers	1075:1080	arg1	parameters					968:977	plant growth parameters	955:977	plant growth parameters	955:977	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	58	theme	tubers	1075:1080	arg1	composition					1060:1070	the chemical composition	1047:1070	the chemical composition of tubers	1047:1080	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	9	59	theme	VvCLC	1456:1460	arg1	channel					1462:1468	the VvCLC channel	1452:1468	the VvCLC channel	1452:1468	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	5	60	dep	VvCLC	675:679	arg1	channel					693:699	a chloride channel	682:699	a chloride channel from Vitis vinifera	682:719	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	5	61	from	vinifera	712:719	arg1	channel					693:699	a chloride channel	682:699	a chloride channel from Vitis vinifera	682:719	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	9	62	theme	greater	1476:1482	arg1	understanding					1484:1496	a greater understanding	1474:1496	a greater understanding of the effect of their overexpression in potato plants	1474:1551	Our findings provide then insight into the role played by the VvNHX antiport and the VvCLC channel and a greater understanding of the effect of their overexpression in potato plants.
36044822	7	63	theme	growth	961:966	arg1	parameters					968:977	plant growth parameters	955:977	plant growth parameters	955:977	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	63	theme	growth	961:966	arg1	yields					986:991	tuber yields	980:991	tuber yields	980:991	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	63	theme	growth	961:966	arg1	composition					1060:1070	the chemical composition	1047:1070	the chemical composition of tubers	1047:1080	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	7	63	theme	growth	961:966	arg1	characteristics					997:1011	characteristics	997:1011	characteristics	997:1011	To this aim, the evaluation of plant growth parameters, tuber yields and characteristics (calibers, eye number and color), the chemical composition of tubers, was conducted and compared between the different lines.
36044822	8	64	theme	vigor	1249:1253	arg1	precocity					1230:1238	flowering precocity	1220:1238	flowering precocity	1220:1238	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	8	64	theme	vigor	1249:1253	arg1	gain					1241:1244	gain	1241:1244	gain of vigor	1241:1253	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	8	64	theme	vigor	1249:1253	arg1	growth					1277:1282	better vegetative growth	1259:1282	better vegetative growth	1259:1282	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	8	65	theme	flowering	1220:1228	arg1	precocity					1230:1238	flowering precocity	1220:1238	flowering precocity	1220:1238	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	8	66	theme	increased	1331:1339	arg1	protein					1341:1347	increased protein and starch contents	1331:1367	protein	1341:1347	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	8	67	theme	tuber	1305:1309	arg1	yields					1311:1316	enhanced tuber yields	1296:1316	enhanced tuber yields	1296:1316	The obtained results showed that transgenic plants displayed an improved growth (flowering precocity, gain of vigor and better vegetative growth) along with enhanced tuber yields and quality (increased protein and starch contents).
36044822	0	68	theme	plants	55:60	arg1	growth					9:14	Improved growth	0:14	Improved growth	0:14	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	0	68	theme	plants	55:60	arg1	quality					26:32	tuber quality	20:32	tuber quality	20:32	Improved growth and tuber quality of transgenic potato plants overexpressing either NHX antiporter, CLC chloride channel, or both.
36044822	5	69	dep	VvNHX	619:623	arg1	antiporter					642:651	a sodium proton antiporter	626:651	a sodium proton antiporter from Vitis vinifera	626:671	To improve both potato growth and behavior under harsh environmental conditions, we produced transgenic potato plants overexpressing either VvNHX (a sodium proton antiporter from Vitis vinifera), VvCLC (a chloride channel from Vitis vinifera), or both.
36044822	2	70	dep	considered	229:238	arg1	As					220:221	As	220:221	As	220:221	As it is considered a worldwide basic vegetarian nutrition to maintain health.
35357965	0	0	from	Variables	47:55	arg1	Spectrometry					65:76	Mass Spectrometry	60:76	Mass Spectrometry of Stromal Matrices	60:96	Decellularization Detergents As Methodological Variables in Mass Spectrometry of Stromal Matrices.
35357965	10	1	theme	matrices	1953:1960	arg1	number					1906:1911	a number	1904:1911	a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs	1904:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	3	2	from	influence	515:523	arg1	proteins					570:577	these proteins	564:577	these proteins	564:577	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	4	3	from	dependence	978:987	arg1	detergent					997:1005	each detergent	992:1005	each detergent	992:1005	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	4	theme	chromatography-mass	889:907	arg1	spectrometry					909:920	liquid chromatography-mass spectrometry	882:920	liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies	882:972	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	5	theme	peculiar	1999:2006	arg1	inputs					2024:2029	peculiar mechanochemical inputs	1999:2029	peculiar mechanochemical inputs	1999:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	4	6	theme	stromal	848:854	arg1	proteins					856:863	stromal proteins	848:863	stromal proteins	848:863	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	6	7	theme	SDS-dependent	1249:1261	arg1	protocol					1263:1270	the SDS-dependent protocol	1245:1270	the SDS-dependent protocol	1245:1270	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	8	8	from	type	1393:1396	arg1	decellularization					1420:1436	organ decellularization	1414:1436	organ decellularization	1414:1436	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	6	9	dep	kidney	1181:1186	arg1	except					1171:1176	except	1171:1176	except	1171:1176	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	1	10	theme	extracellular	179:191	arg1	matrix					193:198	extracellular matrix	179:198	extracellular matrix	179:198	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	4	11	theme	scanning	943:950	arg1	microscopies					961:972	scanning electron microscopies	943:972	scanning electron microscopies	943:972	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	12	theme	quick	633:637	arg1	protocol					690:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	13	theme	inputs	2024:2029	arg1	retainment					1985:1994	selective retainment	1975:1994	selective retainment of peculiar mechanochemical inputs	1975:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	5	14	theme	significant	1089:1099	arg1	level					1101:1105	a statistically significant level	1073:1105	a statistically significant level using the TX100-based protocol	1073:1136	In all organs tested except pancreas, collagens were retained to a statistically significant level using the TX100-based protocol.
35357965	3	15	theme	porcine	582:588	arg1	kidney					590:595	porcine kidney	582:595	porcine kidney	582:595	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	9	16	theme	stromal	1705:1711	arg1	proteins					1713:1720	the stromal proteins	1701:1720	the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices	1701:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	4	17	theme	microwave-assisted	653:670	arg1	protocol					690:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	18	theme	methodological	1832:1845	arg1	variable					1847:1854	This remarkable methodological variable	1816:1854	This remarkable methodological variable	1816:1854	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	1	19	theme	membrane	213:220	arg1	constituents					159:170	key constituents	155:170	key constituents of the extracellular matrix and basement membrane of mammalian organs	155:240	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	1	19	theme	membrane	213:220	arg1	Collagens					99:107	Collagens	99:107	Collagens	99:107	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	9	20	theme	qualitative	1734:1744	arg1	spectrometry					1772:1783	qualitative and semiquantitative mass spectrometry	1734:1783	qualitative and semiquantitative mass spectrometry in acellular porcine matrices	1734:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	6	21	dep	liver	1211:1215	arg1	only					1203:1206	only	1203:1206	only	1203:1206	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	10	22	theme	cells	2085:2089	arg1	development					2059:2069	the development	2055:2069	the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed	2055:2158	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	9	23	theme	semiquantitative	1750:1765	arg1	spectrometry					1772:1783	qualitative and semiquantitative mass spectrometry	1734:1783	qualitative and semiquantitative mass spectrometry in acellular porcine matrices	1734:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	8	24	theme	morphoregulatory	1480:1495	arg1	inputs					1497:1502	morphoregulatory inputs	1480:1502	morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed	1480:1579	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	7	25	theme	irrelevant	1339:1348	arg1	level					1350:1354	an irrelevant level	1336:1354	an irrelevant level	1336:1354	Irrespective of the detergent used, laminin always remained at an irrelevant level.
35357965	3	26	from	requirement	396:406	arg1	engineering					487:497	tissue engineering	480:497	tissue engineering	480:497	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	8	27	theme	peculiar	1504:1511	arg1	inputs					1497:1502	morphoregulatory inputs	1480:1502	morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed	1480:1579	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	4	28	theme	sodium	729:734	arg1	sulfate					744:750	sodium dodecyl sulfate	729:750	sodium dodecyl sulfate (SDS)	729:756	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	28	theme	sodium	729:734	arg1	detergents					717:726	two different detergents	703:726	two different detergents	703:726	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	28	theme	sodium	729:734	arg1	SDS					753:755	SDS	753:755	SDS	753:755	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	0	29	theme	Matrices	89:96	arg1	Spectrometry					65:76	Mass Spectrometry	60:76	Mass Spectrometry of Stromal Matrices	60:96	Decellularization Detergents As Methodological Variables in Mass Spectrometry of Stromal Matrices.
35357965	1	30	theme	key	155:157	arg1	constituents					159:170	key constituents	155:170	key constituents of the extracellular matrix and basement membrane of mammalian organs	155:240	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	1	30	theme	key	155:157	arg1	Collagens					99:107	Collagens	99:107	Collagens	99:107	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	9	31	theme	acellular	1788:1796	arg1	matrices					1806:1813	acellular porcine matrices	1788:1813	acellular porcine matrices	1788:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	5	32	theme	TX100-based	1117:1127	arg1	protocol					1129:1136	the TX100-based protocol	1113:1136	the TX100-based protocol	1113:1136	In all organs tested except pancreas, collagens were retained to a statistically significant level using the TX100-based protocol.
35357965	3	33	theme	matrix-based	435:446	arg1	techniques					466:475	three-dimensional (3D) matrix-based recellularization techniques	412:475	three-dimensional (3D) matrix-based recellularization techniques	412:475	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	8	34	theme	bioartificial	1531:1543	arg1	organ					1555:1559	3D bioartificial mammalian organ	1528:1559	3D bioartificial mammalian organ	1528:1559	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	3	35	dep	three-dimensional	412:428	arg1	3D					431:432	3D	431:432	3D	431:432	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	9	36	theme	Impact	1582:1587	arg1	change					1606:1611	Impact statement Simple change	1582:1611	Impact statement Simple change of the protocol's main detergent	1582:1644	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	3	37	theme	stromal	371:377	arg1	composition					379:389	a native stromal composition	362:389	a native stromal composition	362:389	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	8	38	theme	organ	1555:1559	arg1	type					1520:1523	the type	1516:1523	the type of 3D bioartificial mammalian organ to be reconstructed	1516:1579	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	3	39	theme	tissue	480:485	arg1	engineering					487:497	tissue engineering	480:497	tissue engineering	480:497	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	9	40	theme	Simple	1599:1604	arg1	change					1606:1611	Impact statement Simple change	1582:1611	Impact statement Simple change of the protocol's main detergent	1582:1644	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	3	41	theme	three-dimensional	412:428	arg1	techniques					466:475	three-dimensional (3D) matrix-based recellularization techniques	412:475	three-dimensional (3D) matrix-based recellularization techniques	412:475	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	10	42	theme	reference	1884:1892	arg1	panels					1894:1899	proteomic reference panels	1874:1899	proteomic reference panels	1874:1899	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	8	43	theme	detergent	1401:1409	arg1	type					1393:1396	the type	1389:1396	the type of detergent in organ decellularization	1389:1436	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	4	44	dep	detected	870:877	arg1	Using					625:629	Using	625:629	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100)	625:780	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	9	45	from	spectrometry	1772:1783	arg1	matrices					1806:1813	acellular porcine matrices	1788:1813	acellular porcine matrices	1788:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	4	46	located	detected	870:877	arg1	dependence					978:987	dependence	978:987	dependence on each detergent	978:1005	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	46	located	detected	870:877	arg1	concentration					796:808	identical concentration	786:808	identical concentration	786:808	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	46	located	detected	870:877	arg2	variations					811:820	variations	811:820	variations in matrix conservation of stromal proteins	811:863	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	47	theme	different	1916:1924	arg1	matrices					1953:1960	different species-specific acellular matrices	1916:1960	different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs	1916:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	8	48	theme	organ	1414:1418	arg1	decellularization					1420:1436	organ decellularization	1414:1436	organ decellularization	1414:1436	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	2	49	theme	cells	334:338	arg1	regulation					311:320	the mechanochemical regulation	291:320	the mechanochemical regulation of resident cells	291:338	Thus, changes in their quantities may influence the mechanochemical regulation of resident cells.
35357965	9	50	theme	main	1631:1634	arg1	detergent					1636:1644	the protocol's main detergent	1616:1644	the protocol's main detergent	1616:1644	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	1	51	dep	matrix	193:198	arg1	the					175:177	the	175:177	the	175:177	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	1	52	theme	organs	235:240	arg1	matrix					193:198	extracellular matrix	179:198	extracellular matrix	179:198	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	1	52	theme	organs	235:240	arg1	membrane					213:220	basement membrane	204:220	basement membrane	204:220	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	10	53	theme	acellular	1943:1951	arg1	matrices					1953:1960	different species-specific acellular matrices	1916:1960	different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs	1916:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	9	54	theme	substantial	1662:1672	arg1	difference					1674:1683	a very substantial difference	1655:1683	a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices	1655:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	3	55	theme	detergents	550:559	arg1	influence					515:523	the influence	511:523	the influence of the decellularization detergents on these proteins	511:577	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	0	56	theme	Mass	60:63	arg1	Spectrometry					65:76	Mass Spectrometry	60:76	Mass Spectrometry of Stromal Matrices	60:96	Decellularization Detergents As Methodological Variables in Mass Spectrometry of Stromal Matrices.
35357965	4	57	theme	decellularization	672:688	arg1	protocol					690:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	58	theme	selective	1975:1983	arg1	retainment					1985:1994	selective retainment	1975:1994	selective retainment of peculiar mechanochemical inputs	1975:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	4	59	theme	proteins	856:863	arg1	conservation					832:843	matrix conservation	825:843	matrix conservation of stromal proteins	825:863	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	2	60	theme	mechanochemical	295:309	arg1	regulation					311:320	the mechanochemical regulation	291:320	the mechanochemical regulation of resident cells	291:338	Thus, changes in their quantities may influence the mechanochemical regulation of resident cells.
35357965	1	61	theme	matrix	193:198	arg1	constituents					159:170	key constituents	155:170	key constituents of the extracellular matrix and basement membrane of mammalian organs	155:240	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	1	61	theme	matrix	193:198	arg1	Collagens					99:107	Collagens	99:107	Collagens	99:107	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	9	62	from	difference	1674:1683	arg1	panel					1692:1696	the panel	1688:1696	the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices	1688:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	10	63	theme	mechanochemical	2008:2022	arg1	inputs					2024:2029	peculiar mechanochemical inputs	1999:2029	peculiar mechanochemical inputs	1999:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	1	64	theme	basement	204:211	arg1	membrane					213:220	basement membrane	204:220	basement membrane	204:220	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	9	65	theme	proteins	1713:1720	arg1	panel					1692:1696	the panel	1688:1696	the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices	1688:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	4	66	theme	thawing/quick	639:651	arg1	protocol					690:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	a quick thawing/quick microwave-assisted decellularization protocol	631:697	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	67	theme	remarkable	1821:1830	arg1	variable					1847:1854	This remarkable methodological variable	1816:1854	This remarkable methodological variable	1816:1854	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	4	68	theme	dodecyl	736:742	arg1	sulfate					744:750	sodium dodecyl sulfate	729:750	sodium dodecyl sulfate (SDS)	729:756	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	68	theme	dodecyl	736:742	arg1	detergents					717:726	two different detergents	703:726	two different detergents	703:726	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	68	theme	dodecyl	736:742	arg1	SDS					753:755	SDS	753:755	SDS	753:755	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	69	theme	identical	786:794	arg1	concentration					796:808	identical concentration	786:808	identical concentration	786:808	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	70	theme	proteomic	1874:1882	arg1	panels					1894:1899	proteomic reference panels	1874:1899	proteomic reference panels	1874:1899	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	4	71	from	variations	811:820	arg1	conservation					832:843	matrix conservation	825:843	matrix conservation of stromal proteins	825:863	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	72	theme	liquid	882:887	arg1	spectrometry					909:920	liquid chromatography-mass spectrometry	882:920	liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies	882:972	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	73	theme	seeded	2078:2083	arg1	cells					2085:2089	the seeded cells	2074:2089	the seeded cells	2074:2089	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	0	74	theme	Stromal	81:87	arg1	Matrices					89:96	Stromal Matrices	81:96	Stromal Matrices	81:96	Decellularization Detergents As Methodological Variables in Mass Spectrometry of Stromal Matrices.
35357965	9	75	theme	mass	1767:1770	arg1	spectrometry					1772:1783	qualitative and semiquantitative mass spectrometry	1734:1783	qualitative and semiquantitative mass spectrometry in acellular porcine matrices	1734:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	4	76	theme	different	707:715	arg1	sulfate					744:750	sodium dodecyl sulfate	729:750	sodium dodecyl sulfate (SDS)	729:756	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	76	theme	different	707:715	arg1	X-100					768:772	Triton X-100	761:772	Triton X-100 (TX100)	761:780	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	76	theme	different	707:715	arg1	detergents					717:726	two different detergents	703:726	two different detergents	703:726	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	9	77	theme	porcine	1798:1804	arg1	matrices					1806:1813	acellular porcine matrices	1788:1813	acellular porcine matrices	1788:1813	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	3	78	theme	native	364:369	arg1	composition					379:389	a native stromal composition	362:389	a native stromal composition	362:389	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	8	79	theme	3D	1528:1529	arg1	organ					1555:1559	3D bioartificial mammalian organ	1528:1559	3D bioartificial mammalian organ	1528:1559	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	8	80	theme	mammalian	1545:1553	arg1	organ					1555:1559	3D bioartificial mammalian organ	1528:1559	3D bioartificial mammalian organ	1528:1559	Our results prompt attention to the type of detergent in organ decellularization, suggesting that its choice may influence morphoregulatory inputs peculiar to the type of 3D bioartificial mammalian organ to be reconstructed.
35357965	6	81	from	decorin	1194:1200	arg1	kidney					1181:1186	kidney	1181:1186	kidney	1181:1186	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	6	81	from	decorin	1194:1200	arg1	liver					1211:1215	liver	1211:1215	liver	1211:1215	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	3	82	theme	recellularization	448:464	arg1	techniques					466:475	three-dimensional (3D) matrix-based recellularization techniques	412:475	three-dimensional (3D) matrix-based recellularization techniques	412:475	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	10	83	from	development	2059:2069	arg1	relation					2094:2101	relation	2094:2101	relation to the type of organ to be bioartificially reconstructed	2094:2158	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	0	84	theme	Methodological	32:45	arg1	Variables					47:55	Methodological Variables	32:55	Methodological Variables in Mass Spectrometry of Stromal Matrices	32:96	Decellularization Detergents As Methodological Variables in Mass Spectrometry of Stromal Matrices.
35357965	9	85	theme	statement	1589:1597	arg1	change					1606:1611	Impact statement Simple change	1582:1611	Impact statement Simple change of the protocol's main detergent	1582:1644	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	3	86	theme	composition	379:389	arg1	requirement					396:406	a requirement	394:406	a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering	394:497	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	3	86	theme	composition	379:389	arg1	maintenance					347:357	maintenance	347:357	maintenance of a native stromal composition	347:389	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35357965	4	87	theme	Triton	761:766	arg1	TX100					775:779	TX100	775:779	TX100	775:779	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	87	theme	Triton	761:766	arg1	X-100					768:772	Triton X-100	761:772	Triton X-100 (TX100)	761:780	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	4	87	theme	Triton	761:766	arg1	detergents					717:726	two different detergents	703:726	two different detergents	703:726	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	2	88	from	changes	249:255	arg1	quantities					266:275	their quantities	260:275	their quantities	260:275	Thus, changes in their quantities may influence the mechanochemical regulation of resident cells.
35357965	7	89	theme	detergent	1293:1301	arg1	Irrespective					1273:1284	Irrespective	1273:1284	Irrespective	1273:1284	Irrespective of the detergent used, laminin always remained at an irrelevant level.
35357965	6	90	from	fibrillin	1151:1159	arg1	kidney					1181:1186	kidney	1181:1186	kidney	1181:1186	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	6	90	from	fibrillin	1151:1159	arg1	liver					1211:1215	liver	1211:1215	liver	1211:1215	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	4	91	theme	electron	952:959	arg1	microscopies					961:972	scanning electron microscopies	943:972	scanning electron microscopies	943:972	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	10	92	theme	species-specific	1926:1941	arg1	matrices					1953:1960	different species-specific acellular matrices	1916:1960	different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs	1916:2029	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	4	93	theme	matrix	825:830	arg1	conservation					832:843	matrix conservation	825:843	matrix conservation of stromal proteins	825:863	Using a quick thawing/quick microwave-assisted decellularization protocol and two different detergents, sodium dodecyl sulfate (SDS) vs Triton X-100 (TX100), at identical concentration, variations in matrix conservation of stromal proteins were detected by liquid chromatography-mass spectrometry coupled to light and scanning electron microscopies, in dependence on each detergent.
35357965	6	94	from	elastin	1162:1168	arg1	kidney					1181:1186	kidney	1181:1186	kidney	1181:1186	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	6	94	from	elastin	1162:1168	arg1	liver					1211:1215	liver	1211:1215	liver	1211:1215	In contrast fibrillin, elastin (except in kidney), and decorin (only in liver) were better preserved with the SDS-dependent protocol.
35357965	10	95	theme	organ	2118:2122	arg1	type					2110:2113	the type	2106:2113	the type of organ to be bioartificially reconstructed	2106:2158	This remarkable methodological variable promises to yield proteomic reference panels in a number of different species-specific acellular matrices allowing for selective retainment of peculiar mechanochemical inputs, to differently address the development of the seeded cells in relation to the type of organ to be bioartificially reconstructed.
35357965	2	96	theme	resident	325:332	arg1	cells					334:338	resident cells	325:338	resident cells	325:338	Thus, changes in their quantities may influence the mechanochemical regulation of resident cells.
35357965	1	97	theme	mammalian	225:233	arg1	organs					235:240	mammalian organs	225:240	mammalian organs	225:240	Collagens, elastin, fibrillin, decorin, and laminin are key constituents of the extracellular matrix and basement membrane of mammalian organs.
35357965	9	98	theme	detergent	1636:1644	arg1	change					1606:1611	Impact statement Simple change	1582:1611	Impact statement Simple change of the protocol's main detergent	1582:1644	Impact statement Simple change of the protocol's main detergent leads to a very substantial difference in the panel of the stromal proteins detected by qualitative and semiquantitative mass spectrometry in acellular porcine matrices.
35357965	3	99	theme	decellularization	532:548	arg1	detergents					550:559	the decellularization detergents	528:559	the decellularization detergents	528:559	Since maintenance of a native stromal composition is a requirement for three-dimensional (3D) matrix-based recellularization techniques in tissue engineering, we studied the influence of the decellularization detergents on these proteins in porcine kidney, liver, pancreas, and skin.
35978410	4	0	theme	polysaccharides	884:898	arg1	activities					857:866	physiochemical and biological activities	827:866	physiochemical and biological activities of P. cyrtonema polysaccharides (PCP)	827:904	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	10	1	theme	NO	2165:2166	arg1	production					2168:2177	NO production	2165:2177	NO production	2165:2177	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	11	2	from	bioactivities	2279:2291	arg1	cyrtonema					2320:2328	P. cyrtonema	2317:2328	P. cyrtonema	2317:2328	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	12	3	theme	optimal	2478:2484	arg1	choice					2486:2491	the optimal choice	2474:2491	the optimal choice	2474:2491	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	10	4	theme	Steamed	2012:2018	arg1	PCP					2020:2022	Steamed PCP	2012:2022	Steamed PCP	2012:2022	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	1	5	from	medicine	296:303	arg1	China					308:312	China	308:312	China	308:312	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	4	6	theme	P.	871:872	arg1	polysaccharides					884:898	P. cyrtonema polysaccharides	871:898	P. cyrtonema polysaccharides (PCP)	871:904	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	6	theme	P.	871:872	arg1	PCP					901:903	PCP	901:903	PCP	901:903	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	10	7	theme	scavenging	2048:2057	arg1	activity					2059:2066	scavenging activity	2048:2066	scavenging activity against ABTS radicals	2048:2088	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	1	8	theme	Rhizoma	200:206	arg1	origins					178:184	origins	178:184	origins of Polygonata Rhizoma (HuangJing in Chinese)	178:229	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	11	9	dep	CONCLUSIONS	2197:2207	arg1	summary					2212:2218	summary	2212:2218	summary	2212:2218	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	5	10	theme	molecular	1057:1065	arg1	distribution					1074:1085	Their molecular weight distribution	1051:1085	Their molecular weight distribution	1051:1085	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	7	11	theme	molecular	1653:1661	arg1	weights					1663:1669	all polysaccharides' molecular weights	1632:1669	all polysaccharides' molecular weights	1632:1669	RESULTS Results suggested that molecular weights could be changed during steam, which increased by first steaming and then decreased with further steaming though all polysaccharides' molecular weights were 105-107 Da.
35978410	3	12	theme	cyrtonema	774:782	arg1	standard					750:757	standard	750:757	standard	750:757	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	1	13	theme	Polygonatum	148:158	arg1	origins					178:184	origins	178:184	origins of Polygonata Rhizoma (HuangJing in Chinese)	178:229	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	1	13	theme	Polygonatum	148:158	arg1	one					171:173	one	171:173	one	171:173	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	1	13	theme	Polygonatum	148:158	arg1	cyrtonema					160:168	BACKGROUND Polygonatum cyrtonema	137:168	BACKGROUND Polygonatum cyrtonema	137:168	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	9	14	theme	monosaccharide	1787:1800	arg1	composition					1802:1812	Their monosaccharide composition	1781:1812	Their monosaccharide composition	1781:1812	Their monosaccharide composition and PACE fingerprints were significantly different after steaming, i.e., galactose increased while glucose and mannose decreased, and β-1,4-Galp appeared while β-1,4-Manp increased, after steaming.
35978410	12	15	theme	PCP	2438:2440	arg1	times					2445:2449	2 times	2443:2449	2 times of continuous steam	2443:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	12	15	theme	PCP	2438:2440	arg1	activities					2424:2433	antioxidant and immunopotentiation activities	2389:2433	antioxidant and immunopotentiation activities of PCP	2389:2440	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	6	16	from	cells	1406:1410	arg1	terms					1415:1419	terms	1415:1419	terms of NO production and phagocytosis	1415:1453	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	6	17	theme	phagocytosis	1442:1453	arg1	terms					1415:1419	terms	1415:1419	terms of NO production and phagocytosis	1415:1453	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	11	18	theme	polysaccharides	2296:2310	arg1	bioactivities					2279:2291	bioactivities	2279:2291	bioactivities	2279:2291	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	11	18	theme	polysaccharides	2296:2310	arg1	composition					2263:2273	chemical composition	2254:2273	chemical composition	2254:2273	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	1	19	theme	herbal	289:294	arg1	medicine					296:303	herbal medicine	289:303	herbal medicine in China	289:312	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	0	20	theme	saccharide	72:81	arg1	analysis					91:98	saccharide mapping analysis	72:98	saccharide mapping analysis	72:98	Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays.
35978410	0	21	from	Effects	0:6	arg1	polysaccharides					20:34	polysaccharides	20:34	polysaccharides from Polygonatum cyrtonema	20:61	Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays.
35978410	5	22	theme	distribution	1074:1085	arg1	fingerprints					1124:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints	1051:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints	1051:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	12	23	theme	beneficial	2355:2364	arg1	effects					2366:2372	the balance beneficial effects	2343:2372	the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam	2343:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	11	24	dep	composition	2263:2273	arg1	the					2250:2252	the	2250:2252	the	2250:2252	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	10	25	contain	had	2102:2104	arg1	PCP0					2097:2100	PCP0	2097:2100	PCP0	2097:2100	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	10	25	contain	had	2102:2104	arg2	effect					2133:2138	the best immunostimulatory effect	2106:2138	the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis	2106:2194	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	12	26	theme	balance	2347:2353	arg1	effects					2366:2372	the balance beneficial effects	2343:2372	the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam	2343:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	3	27	theme	steam	421:425	arg1	effects					410:416	effects	410:416	effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity	410:587	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	0	28	theme	pharmacological	104:118	arg1	assays					129:134	pharmacological activity assays	104:134	pharmacological activity assays	104:134	Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays.
35978410	5	29	theme	monosaccharide	1088:1101	arg1	composition					1103:1113	monosaccharide composition	1088:1113	monosaccharide composition	1088:1113	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	0	30	theme	steam	11:15	arg1	Effects					0:6	Effects	0:6	Effects of steam on polysaccharides from Polygonatum cyrtonema	0:61	Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays.
35978410	5	31	theme	saccharide	1194:1203	arg1	mapping					1205:1211	saccharide mapping	1194:1211	saccharide mapping	1194:1211	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	6	32	theme	production	1427:1436	arg1	terms					1415:1419	terms	1415:1419	terms of NO production and phagocytosis	1415:1453	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	3	33	theme	saccharide	482:491	arg1	mapping					493:499	saccharide mapping	482:499	saccharide mapping	482:499	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	3	34	theme	physiochemical	665:678	arg1	activities					695:704	the physiochemical and biological activities	661:704	the physiochemical and biological activities of its polysaccharides	661:727	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	4	35	theme	steam	818:822	arg1	effect					808:813	the effect	804:813	the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP)	804:904	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	11	36	from	cyrtonema	2320:2328	arg1	polysaccharides					2296:2310	polysaccharides	2296:2310	polysaccharides from P. cyrtonema	2296:2328	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	11	36	from	cyrtonema	2320:2328	arg1	bioactivities					2279:2291	bioactivities	2279:2291	bioactivities	2279:2291	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	11	36	from	cyrtonema	2320:2328	arg1	composition					2263:2273	chemical composition	2254:2273	chemical composition	2254:2273	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	3	37	theme	powerful	504:511	arg1	method					513:518	a powerful method	502:518	a powerful method developed for polysaccharides analysis	502:557	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	5	38	theme	carbohydrate	1256:1267	arg1	PACE					1290:1293	PACE	1290:1293	PACE	1290:1293	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	5	38	theme	carbohydrate	1256:1267	arg1	electrophoresis					1273:1287	carbohydrate gel electrophoresis	1256:1287	carbohydrate gel electrophoresis (PACE)	1256:1294	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	10	39	theme	production	2168:2177	arg1	terms					2156:2160	terms	2156:2160	terms of NO production and phagocytosis	2156:2194	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	7	40	theme	molecular	1501:1509	arg1	weights					1511:1517	molecular weights	1501:1517	molecular weights	1501:1517	RESULTS Results suggested that molecular weights could be changed during steam, which increased by first steaming and then decreased with further steaming though all polysaccharides' molecular weights were 105-107 Da.
35978410	4	41	theme	cyrtonema	874:882	arg1	polysaccharides					884:898	P. cyrtonema polysaccharides	871:898	P. cyrtonema polysaccharides (PCP)	871:904	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	41	theme	cyrtonema	874:882	arg1	PCP					901:903	PCP	901:903	PCP	901:903	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	12	42	theme	steam	2465:2469	arg1	times					2445:2449	2 times	2443:2449	2 times of continuous steam	2443:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	12	42	theme	steam	2465:2469	arg1	activities					2424:2433	antioxidant and immunopotentiation activities	2389:2433	antioxidant and immunopotentiation activities of PCP	2389:2440	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	3	43	theme	biological	684:693	arg1	activities					695:704	the physiochemical and biological activities	661:704	the physiochemical and biological activities of its polysaccharides	661:727	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	3	44	theme	polysaccharides	534:548	arg1	analysis					550:557	polysaccharides analysis	534:557	polysaccharides analysis	534:557	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	5	45	theme	polysaccharides	1222:1236	arg1	analysis					1238:1245	polysaccharides analysis	1222:1245	polysaccharides analysis	1222:1245	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	3	46	from	effect	642:647	arg1	activities					695:704	the physiochemical and biological activities	661:704	the physiochemical and biological activities of its polysaccharides	661:727	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	8	47	theme	aqueous	1742:1748	arg1	solution					1750:1757	aqueous solution	1742:1757	aqueous solution	1742:1757	They all showed irregularly spherical conformation in aqueous solution based on AFM imaging.
35978410	1	48	theme	origins	178:184	arg1	origins					178:184	origins	178:184	origins of Polygonata Rhizoma (HuangJing in Chinese)	178:229	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	1	48	theme	origins	178:184	arg1	one					171:173	one	171:173	one	171:173	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	1	48	theme	origins	178:184	arg1	cyrtonema					160:168	BACKGROUND Polygonatum cyrtonema	137:168	BACKGROUND Polygonatum cyrtonema	137:168	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	7	49	theme	RESULTS	1470:1476	arg1	Results					1478:1484	RESULTS Results	1470:1484	RESULTS Results	1470:1484	RESULTS Results suggested that molecular weights could be changed during steam, which increased by first steaming and then decreased with further steaming though all polysaccharides' molecular weights were 105-107 Da.
35978410	4	50	theme	biological	846:855	arg1	activities					857:866	physiochemical and biological activities	827:866	physiochemical and biological activities of P. cyrtonema polysaccharides (PCP)	827:904	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	10	51	theme	phagocytosis	2183:2194	arg1	terms					2156:2160	terms	2156:2160	terms of NO production and phagocytosis	2156:2194	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	3	52	theme	polysaccharides	713:727	arg1	activities					695:704	the physiochemical and biological activities	661:704	the physiochemical and biological activities of its polysaccharides	661:727	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	3	53	theme	pharmacological	564:578	arg1	activity					580:587	pharmacological activity	564:587	pharmacological activity	564:587	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	3	54	theme	Polygonatum	762:772	arg1	cyrtonema					774:782	Polygonatum cyrtonema	762:782	Polygonatum cyrtonema	762:782	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	8	55	theme	AFM	1768:1770	arg1	imaging					1772:1778	AFM imaging	1768:1778	AFM imaging	1768:1778	They all showed irregularly spherical conformation in aqueous solution based on AFM imaging.
35978410	4	56	theme	named	927:931	arg1	PCP0					933:936	named PCP0	927:936	named PCP0	927:936	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	56	theme	named	927:931	arg1	polysaccharides					911:925	six polysaccharides	907:925	six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5	907:969	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	6	57	theme	antioxidant	1340:1350	arg1	ability					1352:1358	their antioxidant ability and immunostimulatory activities	1334:1391	ability	1352:1358	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	12	58	theme	continuous	2454:2463	arg1	steam					2465:2469	continuous steam	2454:2469	continuous steam	2454:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	10	59	theme	ABTS	2076:2079	arg1	radicals					2081:2088	ABTS radicals	2076:2088	ABTS radicals	2076:2088	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	5	60	theme	weight	1067:1072	arg1	distribution					1074:1085	Their molecular weight distribution	1051:1085	Their molecular weight distribution	1051:1085	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	1	61	theme	BACKGROUND	137:146	arg1	origins					178:184	origins	178:184	origins of Polygonata Rhizoma (HuangJing in Chinese)	178:229	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	1	61	theme	BACKGROUND	137:146	arg1	one					171:173	one	171:173	one	171:173	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	1	61	theme	BACKGROUND	137:146	arg1	cyrtonema					160:168	BACKGROUND Polygonatum cyrtonema	137:168	BACKGROUND Polygonatum cyrtonema	137:168	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	3	62	from	cyrtonema	463:471	arg1	polysaccharides					430:444	polysaccharides	430:444	polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity	430:587	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	4	63	dep	polysaccharides	911:925	arg1	PCP0					933:936	named PCP0	927:936	named PCP0	927:936	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	63	dep	polysaccharides	911:925	arg1	PCP1					939:942	PCP1	939:942	PCP1	939:942	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	63	dep	polysaccharides	911:925	arg1	PCP4					957:960	PCP4	957:960	PCP4	957:960	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	63	dep	polysaccharides	911:925	arg1	PCP5					966:969	PCP5	966:969	PCP5	966:969	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	63	dep	polysaccharides	911:925	arg1	polysaccharides					911:925	six polysaccharides	907:925	six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5	907:969	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	63	dep	polysaccharides	911:925	arg1	PCP2					945:948	PCP2	945:948	PCP2	945:948	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	63	dep	polysaccharides	911:925	arg1	PCP3					951:954	PCP3	951:954	PCP3	951:954	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	4	64	from	effect	808:813	arg1	activities					857:866	physiochemical and biological activities	827:866	physiochemical and biological activities of P. cyrtonema polysaccharides (PCP)	827:904	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	12	65	theme	antioxidant	2389:2399	arg1	times					2445:2449	2 times	2443:2449	2 times of continuous steam	2443:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	12	65	theme	antioxidant	2389:2399	arg1	activities					2424:2433	antioxidant and immunopotentiation activities	2389:2433	antioxidant and immunopotentiation activities of PCP	2389:2440	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	0	66	theme	mapping	83:89	arg1	analysis					91:98	saccharide mapping analysis	72:98	saccharide mapping analysis	72:98	Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays.
35978410	5	67	theme	PACE	1119:1122	arg1	fingerprints					1124:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints	1051:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints	1051:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	6	68	from	activities	1382:1391	arg1	cells					1406:1410	RAW 264.7 cells	1396:1410	RAW 264.7 cells in terms of NO production and phagocytosis	1396:1453	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	9	69	theme	PACE	1818:1821	arg1	fingerprints					1823:1834	PACE fingerprints	1818:1834	PACE fingerprints	1818:1834	Their monosaccharide composition and PACE fingerprints were significantly different after steaming, i.e., galactose increased while glucose and mannose decreased, and β-1,4-Galp appeared while β-1,4-Manp increased, after steaming.
35978410	12	70	theme	immunopotentiation	2405:2422	arg1	times					2445:2449	2 times	2443:2449	2 times of continuous steam	2443:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	12	70	theme	immunopotentiation	2405:2422	arg1	activities					2424:2433	antioxidant and immunopotentiation activities	2389:2433	antioxidant and immunopotentiation activities of PCP	2389:2440	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	10	71	theme	immunostimulatory	2115:2131	arg1	effect					2133:2138	the best immunostimulatory effect	2106:2138	the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis	2106:2194	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	6	72	theme	RAW	1396:1398	arg1	cells					1406:1410	RAW 264.7 cells	1396:1410	RAW 264.7 cells in terms of NO production and phagocytosis	1396:1453	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	0	73	from	cyrtonema	53:61	arg1	polysaccharides					20:34	polysaccharides	20:34	polysaccharides from Polygonatum cyrtonema	20:61	Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays.
35978410	0	74	theme	activity	120:127	arg1	assays					129:134	pharmacological activity assays	104:134	pharmacological activity assays	104:134	Effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping analysis and pharmacological activity assays.
35978410	12	75	theme	steaming	2377:2384	arg1	effects					2366:2372	the balance beneficial effects	2343:2372	the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam	2343:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	12	76	from	effects	2366:2372	arg1	times					2445:2449	2 times	2443:2449	2 times of continuous steam	2443:2469	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	12	76	from	effects	2366:2372	arg1	activities					2424:2433	antioxidant and immunopotentiation activities	2389:2433	antioxidant and immunopotentiation activities of PCP	2389:2440	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	5	77	theme	composition	1103:1113	arg1	fingerprints					1124:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints	1051:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints	1051:1135	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	11	78	theme	chemical	2254:2261	arg1	composition					2263:2273	chemical composition	2254:2273	chemical composition	2254:2273	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	2	79	theme	HuangJing	362:370	arg1	steaming					350:357	steaming	350:357	steaming of HuangJing	350:370	However, there has no standard for steaming of HuangJing.
35978410	6	80	from	ability	1352:1358	arg1	cells					1406:1410	RAW 264.7 cells	1396:1410	RAW 264.7 cells in terms of NO production and phagocytosis	1396:1453	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	7	81	theme	first	1569:1573	arg1	steaming					1575:1582	first steaming	1569:1582	first steaming	1569:1582	RESULTS Results suggested that molecular weights could be changed during steam, which increased by first steaming and then decreased with further steaming though all polysaccharides' molecular weights were 105-107 Da.
35978410	1	82	dep	Rhizoma	200:206	arg1	HuangJing					209:217	HuangJing	209:217	HuangJing	209:217	BACKGROUND Polygonatum cyrtonema, one of origins of Polygonata Rhizoma (HuangJing in Chinese), is traditionally steamed repeatedly before being used as herbal medicine in China.
35978410	6	83	theme	immunostimulatory	1364:1380	arg1	activities					1382:1391	their antioxidant ability and immunostimulatory activities	1334:1391	activities	1382:1391	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	6	84	theme	NO	1424:1425	arg1	production					1427:1436	NO production	1424:1436	NO production	1424:1436	In addition, their antioxidant ability and immunostimulatory activities on RAW 264.7 cells in terms of NO production and phagocytosis were compared.
35978410	3	85	theme	comprehensive	386:398	arg1	necessary					599:607	necessary	599:607	necessary	599:607	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	3	85	theme	comprehensive	386:398	arg1	study					400:404	a comprehensive study	384:404	a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity	384:587	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	4	86	theme	physiochemical	827:840	arg1	activities					857:866	physiochemical and biological activities	827:866	physiochemical and biological activities of P. cyrtonema polysaccharides (PCP)	827:904	METHODS To explore the effect of steam on physiochemical and biological activities of P. cyrtonema polysaccharides (PCP), six polysaccharides named PCP0, PCP1, PCP2, PCP3, PCP4 and PCP5 were extracted from the herb consecutively steamed for 0-5 times, respectively.
35978410	10	87	from	effect	2133:2138	arg1	RAW					2143:2145	RAW 264.7	2143:2151	RAW 264.7	2143:2151	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	10	87	from	effect	2133:2138	arg1	terms					2156:2160	terms	2156:2160	terms of NO production and phagocytosis	2156:2194	Steamed PCP significantly increased scavenging activity against ABTS radicals, while PCP0 had the best immunostimulatory effect on RAW 264.7 in terms of NO production and phagocytosis.
35978410	3	88	theme	steam	652:656	arg1	effect					642:647	the effect	638:647	the effect of steam on the physiochemical and biological activities of its polysaccharides	638:727	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	3	89	from	effects	410:416	arg1	polysaccharides					430:444	polysaccharides	430:444	polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity	430:587	Therefore, a comprehensive study for effects of steam on polysaccharides from Polygonatum cyrtonema based on saccharide mapping, a powerful method developed for polysaccharides analysis, and pharmacological activity are still necessary, which is helpful to explore the effect of steam on the physiochemical and biological activities of its polysaccharides and develop steaming standard of Polygonatum cyrtonema.
35978410	12	90	theme	given	2503:2507	arg1	conditions					2509:2518	the given conditions	2499:2518	the given conditions	2499:2518	Considering the balance beneficial effects of steaming on antioxidant and immunopotentiation activities of PCP, 2 times of continuous steam is the optimal choice under the given conditions.
35978410	11	91	from	composition	2263:2273	arg1	cyrtonema					2320:2328	P. cyrtonema	2317:2328	P. cyrtonema	2317:2328	CONCLUSIONS In summary, steam significantly affected the chemical composition and bioactivities of polysaccharides from P. cyrtonema.
35978410	8	92	theme	spherical	1716:1724	arg1	conformation					1726:1737	irregularly spherical conformation	1704:1737	irregularly spherical conformation	1704:1737	They all showed irregularly spherical conformation in aqueous solution based on AFM imaging.
35978410	5	93	theme	gel	1269:1271	arg1	PACE					1290:1293	PACE	1290:1293	PACE	1290:1293	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
35978410	5	93	theme	gel	1269:1271	arg1	electrophoresis					1273:1287	carbohydrate gel electrophoresis	1256:1287	carbohydrate gel electrophoresis (PACE)	1256:1294	Their molecular weight distribution, monosaccharide composition and PACE fingerprints were investigated through HPSEC-MALLS-RID, HPAEC-PAD and saccharide mapping based on polysaccharides analysis by using carbohydrate gel electrophoresis (PACE) and HPTLC, respectively.
36386641	6	0	theme	lung	799:802	arg1	tissues					804:810	lung tissues	799:810	lung tissues	799:810	The results showed that APS pre-treatment improved the pathological changes of lung tissues, reduced the neutrophils infiltration, and inhibited the LPS-induced inflammation.
36386641	3	1	theme	multiple	375:382	arg1	bioactivities					384:396	multiple bioactivities	375:396	multiple bioactivities including anti-inflammation and immunoregulation	375:445	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	3	1	theme	multiple	375:382	arg1	immunoregulation					430:445	immunoregulation	430:445	immunoregulation	430:445	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	3	1	theme	multiple	375:382	arg1	anti-inflammation					408:424	anti-inflammation	408:424	anti-inflammation	408:424	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	8	2	theme	acids	1144:1148	arg1	Akkermansia					1196:1206	Akkermansia	1196:1206	Akkermansia	1196:1206	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	2	theme	acids	1144:1148	arg1	genus					1168:1172	short-chain fatty acids (SCFAs)-producing genus	1126:1172	short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus	1126:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	2	theme	acids	1144:1148	arg1	Oscillospira					1182:1193	Oscillospira	1182:1193	Oscillospira	1182:1193	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	2	theme	acids	1144:1148	arg1	Coprococcus					1213:1223	Coprococcus	1213:1223	Coprococcus	1213:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	10	3	theme	APS	1476:1478	arg1	effects					1465:1471	the preventive effects	1450:1471	the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs	1450:1633	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	1	4	theme	limited	196:202	arg1	effects					216:222	limited therapeutic effects	196:222	limited therapeutic effects	196:222	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	7	5	theme	lung	982:985	arg1	response					1000:1007	lung inflammatory response	982:1007	lung inflammatory response	982:1007	Increasing evidence confirmed the close relationship between intestinal microbiota and lung inflammatory response.
36386641	10	6	theme	lung	1495:1498	arg1	injury					1500:1505	LPS-induced lung injury	1483:1505	LPS-induced lung injury	1483:1505	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	10	7	theme	microbiota	1570:1579	arg1	composition					1581:1591	intestinal microbiota composition	1559:1591	intestinal microbiota composition	1559:1591	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	9	8	theme	SCFAs	1298:1302	arg1	propionate					1327:1336	propionate	1327:1336	propionate	1327:1336	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	9	8	theme	SCFAs	1298:1302	arg1	butyrate					1314:1321	butyrate	1314:1321	butyrate	1314:1321	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	9	8	theme	SCFAs	1298:1302	arg1	concentrations					1280:1293	the serum concentrations	1270:1293	the serum concentrations of SCFAs including butyrate and propionate	1270:1336	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	7	9	theme	inflammatory	987:998	arg1	response					1000:1007	lung inflammatory response	982:1007	lung inflammatory response	982:1007	Increasing evidence confirmed the close relationship between intestinal microbiota and lung inflammatory response.
36386641	1	10	with	disease	183:189	arg1	effects					216:222	limited therapeutic effects	196:222	limited therapeutic effects	196:222	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	4	11	theme	lung	496:499	arg1	injury					501:506	inflammatory lung injury	483:506	inflammatory lung injury	483:506	However, its preventive effects on inflammatory lung injury remain unclear.
36386641	8	12	theme	genus	1168:1172	arg1	abundance					1113:1121	the abundance	1109:1121	the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus	1109:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	4	13	theme	inflammatory	483:494	arg1	injury					501:506	inflammatory lung injury	483:506	inflammatory lung injury	483:506	However, its preventive effects on inflammatory lung injury remain unclear.
36386641	10	14	theme	composition	1581:1591	arg1	increase					1611:1618	the resulting increase	1597:1618	the resulting increase of serum SCFAs	1597:1633	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	10	14	theme	composition	1581:1591	arg1	alteration					1545:1554	the alteration	1541:1554	the alteration of intestinal microbiota composition	1541:1591	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	1	15	theme	therapeutic	204:214	arg1	effects					216:222	limited therapeutic effects	196:222	limited therapeutic effects	196:222	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	10	16	theme	resulting	1601:1609	arg1	increase					1611:1618	the resulting increase	1597:1618	the resulting increase of serum SCFAs	1597:1633	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	8	17	theme	-producing	1157:1166	arg1	Akkermansia					1196:1206	Akkermansia	1196:1206	Akkermansia	1196:1206	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	17	theme	-producing	1157:1166	arg1	genus					1168:1172	short-chain fatty acids (SCFAs)-producing genus	1126:1172	short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus	1126:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	17	theme	-producing	1157:1166	arg1	Oscillospira					1182:1193	Oscillospira	1182:1193	Oscillospira	1182:1193	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	17	theme	-producing	1157:1166	arg1	Coprococcus					1213:1223	Coprococcus	1213:1223	Coprococcus	1213:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	6	18	theme	LPS-induced	869:879	arg1	inflammation					881:892	the LPS-induced inflammation	865:892	the LPS-induced inflammation	865:892	The results showed that APS pre-treatment improved the pathological changes of lung tissues, reduced the neutrophils infiltration, and inhibited the LPS-induced inflammation.
36386641	2	19	theme	lung	325:328	arg1	injury					330:335	inflammatory lung injury	312:335	inflammatory lung injury	312:335	Increasing opinions approved that prevention is more important than drug treatment for inflammatory lung injury.
36386641	5	20	theme	intragastric	573:584	arg1	gavage					586:591	intragastric gavage	573:591	intragastric gavage	573:591	In this study, mice were pretreated with APS via intragastric gavage and then were intratracheally instilled with lipopolysaccharides (LPS) to determine the role of APS in preventing lung injury.
36386641	10	21	from	effects	1465:1471	arg1	injury					1500:1505	LPS-induced lung injury	1483:1505	LPS-induced lung injury	1483:1505	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	10	22	theme	LPS-induced	1483:1493	arg1	injury					1500:1505	LPS-induced lung injury	1483:1505	LPS-induced lung injury	1483:1505	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	5	23	theme	lung	707:710	arg1	injury					712:717	lung injury	707:717	lung injury	707:717	In this study, mice were pretreated with APS via intragastric gavage and then were intratracheally instilled with lipopolysaccharides (LPS) to determine the role of APS in preventing lung injury.
36386641	7	24	theme	intestinal	956:965	arg1	microbiota					967:976	intestinal microbiota	956:976	intestinal microbiota	956:976	Increasing evidence confirmed the close relationship between intestinal microbiota and lung inflammatory response.
36386641	0	25	theme	Astragalus	0:9	arg1	polysaccharides					11:25	Astragalus polysaccharides	0:25	Astragalus polysaccharides	0:25	Astragalus polysaccharides alleviates lipopolysaccharides-induced inflammatory lung injury by altering intestinal microbiota in mice.
36386641	7	26	theme	Increasing	895:904	arg1	evidence					906:913	Increasing evidence	895:913	Increasing evidence	895:913	Increasing evidence confirmed the close relationship between intestinal microbiota and lung inflammatory response.
36386641	4	27	theme	preventive	461:470	arg1	effects					472:478	its preventive effects	457:478	its preventive effects on inflammatory lung injury	457:506	However, its preventive effects on inflammatory lung injury remain unclear.
36386641	7	28	theme	close	929:933	arg1	relationship					935:946	the close relationship	925:946	the close relationship between intestinal microbiota and lung inflammatory response	925:1007	Increasing evidence confirmed the close relationship between intestinal microbiota and lung inflammatory response.
36386641	9	29	theme	anti-inflammation	1349:1365	arg1	effects					1367:1373	their anti-inflammation effects	1343:1373	their anti-inflammation effects	1343:1373	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	0	30	theme	lipopolysaccharides-induced	38:64	arg1	injury					84:89	lipopolysaccharides-induced inflammatory lung injury	38:89	lipopolysaccharides-induced inflammatory lung injury	38:89	Astragalus polysaccharides alleviates lipopolysaccharides-induced inflammatory lung injury by altering intestinal microbiota in mice.
36386641	8	31	theme	fatty	1138:1142	arg1	SCFAs					1151:1155	SCFAs	1151:1155	SCFAs	1151:1155	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	31	theme	fatty	1138:1142	arg1	acids					1144:1148	short-chain fatty acids	1126:1148	short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus	1126:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	5	32	theme	APS	689:691	arg1	role					681:684	the role	677:684	the role of APS in preventing lung injury	677:717	In this study, mice were pretreated with APS via intragastric gavage and then were intratracheally instilled with lipopolysaccharides (LPS) to determine the role of APS in preventing lung injury.
36386641	1	33	theme	Inflammatory	134:145	arg1	disease					183:189	a common respiratory disease	162:189	a common respiratory disease with limited therapeutic effects	162:222	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	1	33	theme	Inflammatory	134:145	arg1	injury					152:157	Inflammatory lung injury	134:157	Inflammatory lung injury	134:157	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	8	34	theme	16S	1010:1012	arg1	rRNA					1014:1017	16S rRNA	1010:1017	16S rRNA analysis	1010:1026	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	3	35	theme	Astragalus	338:347	arg1	APS					366:368	APS	366:368	APS	366:368	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	3	35	theme	Astragalus	338:347	arg1	polysaccharides					349:363	Astragalus polysaccharides	338:363	Astragalus polysaccharides (APS)	338:369	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	1	36	theme	lung	147:150	arg1	disease					183:189	a common respiratory disease	162:189	a common respiratory disease with limited therapeutic effects	162:222	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	1	36	theme	lung	147:150	arg1	injury					152:157	Inflammatory lung injury	134:157	Inflammatory lung injury	134:157	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	6	37	theme	APS	744:746	arg1	pre-treatment					748:760	APS pre-treatment	744:760	APS pre-treatment	744:760	The results showed that APS pre-treatment improved the pathological changes of lung tissues, reduced the neutrophils infiltration, and inhibited the LPS-induced inflammation.
36386641	8	38	theme	rRNA	1014:1017	arg1	analysis					1019:1026	16S rRNA analysis	1010:1026	16S rRNA analysis	1010:1026	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	0	39	theme	lung	79:82	arg1	injury					84:89	lipopolysaccharides-induced inflammatory lung injury	38:89	lipopolysaccharides-induced inflammatory lung injury	38:89	Astragalus polysaccharides alleviates lipopolysaccharides-induced inflammatory lung injury by altering intestinal microbiota in mice.
36386641	10	40	theme	SCFAs	1629:1633	arg1	increase					1611:1618	the resulting increase	1597:1618	the resulting increase of serum SCFAs	1597:1633	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	10	40	theme	SCFAs	1629:1633	arg1	alteration					1545:1554	the alteration	1541:1554	the alteration of intestinal microbiota composition	1541:1591	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	0	41	from	microbiota	114:123	arg1	mice					128:131	mice	128:131	mice	128:131	Astragalus polysaccharides alleviates lipopolysaccharides-induced inflammatory lung injury by altering intestinal microbiota in mice.
36386641	8	42	from	composition	1077:1087	arg1	colon					1092:1096	colon	1092:1096	colon	1092:1096	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	0	43	theme	inflammatory	66:77	arg1	injury					84:89	lipopolysaccharides-induced inflammatory lung injury	38:89	lipopolysaccharides-induced inflammatory lung injury	38:89	Astragalus polysaccharides alleviates lipopolysaccharides-induced inflammatory lung injury by altering intestinal microbiota in mice.
36386641	10	44	theme	serum	1623:1627	arg1	SCFAs					1629:1633	serum SCFAs	1623:1633	serum SCFAs	1623:1633	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	8	45	theme	APS	1040:1042	arg1	treatment					1044:1052	APS treatment	1040:1052	APS treatment	1040:1052	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	10	46	theme	intestinal	1559:1568	arg1	composition					1581:1591	intestinal microbiota composition	1559:1591	intestinal microbiota composition	1559:1591	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	9	47	theme	APS	1232:1234	arg1	treatment					1236:1244	APS treatment	1232:1244	APS treatment	1232:1244	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	6	48	theme	neutrophils	825:835	arg1	infiltration					837:848	the neutrophils infiltration	821:848	the neutrophils infiltration	821:848	The results showed that APS pre-treatment improved the pathological changes of lung tissues, reduced the neutrophils infiltration, and inhibited the LPS-induced inflammation.
36386641	8	49	theme	short-chain	1126:1136	arg1	SCFAs					1151:1155	SCFAs	1151:1155	SCFAs	1151:1155	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	8	49	theme	short-chain	1126:1136	arg1	acids					1144:1148	short-chain fatty acids	1126:1148	short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus	1126:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	2	50	theme	inflammatory	312:323	arg1	injury					330:335	inflammatory lung injury	312:335	inflammatory lung injury	312:335	Increasing opinions approved that prevention is more important than drug treatment for inflammatory lung injury.
36386641	10	51	theme	preventive	1454:1463	arg1	effects					1465:1471	the preventive effects	1450:1471	the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs	1450:1633	Our data confirmed the preventive effects of APS on LPS-induced lung injury, which were partly contributed by the alteration of intestinal microbiota composition and the resulting increase of serum SCFAs.
36386641	9	52	theme	serum	1274:1278	arg1	propionate					1327:1336	propionate	1327:1336	propionate	1327:1336	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	9	52	theme	serum	1274:1278	arg1	butyrate					1314:1321	butyrate	1314:1321	butyrate	1314:1321	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	9	52	theme	serum	1274:1278	arg1	concentrations					1280:1293	the serum concentrations	1270:1293	the serum concentrations of SCFAs including butyrate and propionate	1270:1336	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	4	53	from	effects	472:478	arg1	injury					501:506	inflammatory lung injury	483:506	inflammatory lung injury	483:506	However, its preventive effects on inflammatory lung injury remain unclear.
36386641	9	54	theme	mice	1396:1399	arg1	macrophages					1418:1428	mice primary alveolar macrophages	1396:1428	mice primary alveolar macrophages	1396:1428	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	8	55	theme	microbiota	1066:1075	arg1	composition					1077:1087	the microbiota composition	1062:1087	the microbiota composition in colon	1062:1096	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
36386641	1	56	theme	common	164:169	arg1	disease					183:189	a common respiratory disease	162:189	a common respiratory disease with limited therapeutic effects	162:222	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	1	56	theme	common	164:169	arg1	injury					152:157	Inflammatory lung injury	134:157	Inflammatory lung injury	134:157	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	6	57	theme	pathological	775:786	arg1	changes					788:794	the pathological changes	771:794	the pathological changes of lung tissues	771:810	The results showed that APS pre-treatment improved the pathological changes of lung tissues, reduced the neutrophils infiltration, and inhibited the LPS-induced inflammation.
36386641	0	58	theme	intestinal	103:112	arg1	microbiota					114:123	intestinal microbiota	103:123	intestinal microbiota in mice	103:131	Astragalus polysaccharides alleviates lipopolysaccharides-induced inflammatory lung injury by altering intestinal microbiota in mice.
36386641	9	59	theme	primary	1401:1407	arg1	macrophages					1418:1428	mice primary alveolar macrophages	1396:1428	mice primary alveolar macrophages	1396:1428	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	3	60	contain	has	371:373	arg2	immunoregulation					430:445	immunoregulation	430:445	immunoregulation	430:445	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	3	60	contain	has	371:373	arg1	APS					366:368	APS	366:368	APS	366:368	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	3	60	contain	has	371:373	arg2	anti-inflammation					408:424	anti-inflammation	408:424	anti-inflammation	408:424	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	3	60	contain	has	371:373	arg1	polysaccharides					349:363	Astragalus polysaccharides	338:363	Astragalus polysaccharides (APS)	338:369	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	3	60	contain	has	371:373	arg2	bioactivities					384:396	multiple bioactivities	375:396	multiple bioactivities including anti-inflammation and immunoregulation	375:445	Astragalus polysaccharides (APS) has multiple bioactivities including anti-inflammation and immunoregulation.
36386641	1	61	theme	respiratory	171:181	arg1	disease					183:189	a common respiratory disease	162:189	a common respiratory disease with limited therapeutic effects	162:222	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	1	61	theme	respiratory	171:181	arg1	injury					152:157	Inflammatory lung injury	134:157	Inflammatory lung injury	134:157	Inflammatory lung injury is a common respiratory disease with limited therapeutic effects.
36386641	9	62	theme	alveolar	1409:1416	arg1	macrophages					1418:1428	mice primary alveolar macrophages	1396:1428	mice primary alveolar macrophages	1396:1428	Also, APS treatment significantly increased the serum concentrations of SCFAs including butyrate and propionate, and their anti-inflammation effects were demonstrated on mice primary alveolar macrophages.
36386641	2	63	theme	drug	293:296	arg1	treatment					298:306	drug treatment	293:306	drug treatment	293:306	Increasing opinions approved that prevention is more important than drug treatment for inflammatory lung injury.
36386641	6	64	theme	tissues	804:810	arg1	changes					788:794	the pathological changes	771:794	the pathological changes of lung tissues	771:810	The results showed that APS pre-treatment improved the pathological changes of lung tissues, reduced the neutrophils infiltration, and inhibited the LPS-induced inflammation.
36386641	8	65	dep	changed	1054:1060	arg1	increased					1099:1107	increased	1099:1107	increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus	1099:1223	16S rRNA analysis showed that APS treatment changed the microbiota composition in colon, increased the abundance of short-chain fatty acids (SCFAs)-producing genus such as Oscillospira, Akkermansia, and Coprococcus.
35267367	5	0	theme	Type	667:670	arg1	behavior					674:681	Type I behavior	667:681	Type I behavior (inter-cycling strain-thinning)	667:713	All samples exhibited Type I behavior (inter-cycling strain-thinning) and mainly elastic behavior.
35267367	5	0	theme	Type	667:670	arg1	strain-thinning					698:712	inter-cycling strain-thinning	684:712	inter-cycling strain-thinning	684:712	All samples exhibited Type I behavior (inter-cycling strain-thinning) and mainly elastic behavior.
35267367	7	1	theme	a	929:929	arg1	concentration					931:943	Too high a concentration	920:943	Too high a concentration of guar gum	920:955	Too high a concentration of guar gum hinders the formation of protein disulfide bonds.
35267367	4	2	theme	%	599:599	arg1	κ-carrageenan					601:613	0.4% κ-carrageenan	596:613	0.4% κ-carrageenan	596:613	The sample with 0.4% κ-carrageenan has the highest gel strength.
35267367	5	3	theme	I	672:672	arg1	behavior					674:681	Type I behavior	667:681	Type I behavior (inter-cycling strain-thinning)	667:713	All samples exhibited Type I behavior (inter-cycling strain-thinning) and mainly elastic behavior.
35267367	5	3	theme	I	672:672	arg1	strain-thinning					698:712	inter-cycling strain-thinning	684:712	inter-cycling strain-thinning	684:712	All samples exhibited Type I behavior (inter-cycling strain-thinning) and mainly elastic behavior.
35267367	7	4	theme	bonds	1000:1004	arg1	formation					969:977	the formation	965:977	the formation of protein disulfide bonds	965:1004	Too high a concentration of guar gum hinders the formation of protein disulfide bonds.
35267367	3	5	theme	phycocyanin	527:537	arg1	gel					539:541	phycocyanin gel	527:541	phycocyanin gel	527:541	The results show that both κ-carrageenan and guar gum can enhance the network structure of phycocyanin gel and weaken the frequency dependence.
35267367	4	6	theme	0.4	596:598	arg1	%					599:599	%	599:599	%	599:599	The sample with 0.4% κ-carrageenan has the highest gel strength.
35267367	0	7	theme	Phycocyanin	90:100	arg1	Gel					102:104	Phycocyanin Gel	90:104	Phycocyanin Gel	90:104	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	0	8	from	Effects	0:6	arg1	Microstructure					72:85	Microstructure	72:85	Microstructure	72:85	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	0	8	from	Effects	0:6	arg1	Properties					57:66	Rheological Properties	45:66	Rheological Properties	45:66	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	8	9	theme	new	1082:1084	arg1	products					1091:1098	new food products	1082:1098	new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry	1082:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	6	10	theme	essential	846:854	arg1	role					856:859	an essential role	843:859	an essential role	843:859	As the concentration of κ-carrageenan increases, hydrophobic interactions and disulfide bonds play an essential role in maintaining the three-dimensional structure of the gel.
35267367	3	11	theme	frequency	558:566	arg1	dependence					568:577	the frequency dependence	554:577	the frequency dependence	554:577	The results show that both κ-carrageenan and guar gum can enhance the network structure of phycocyanin gel and weaken the frequency dependence.
35267367	7	12	theme	protein	982:988	arg1	bonds					1000:1004	protein disulfide bonds	982:1004	protein disulfide bonds	982:1004	Too high a concentration of guar gum hinders the formation of protein disulfide bonds.
35267367	4	13	with	sample	584:589	arg1	κ-carrageenan					601:613	0.4% κ-carrageenan	596:613	0.4% κ-carrageenan	596:613	The sample with 0.4% κ-carrageenan has the highest gel strength.
35267367	1	14	theme	neutral	269:275	arg1	polysaccharides					277:291	neutral polysaccharides	269:291	neutral polysaccharides (guar gum)	269:302	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	1	14	theme	neutral	269:275	arg1	gum					299:301	guar gum	294:301	guar gum	294:301	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	8	15	with	polysaccharides	1135:1149	arg1	texture					1162:1168	ideal texture	1156:1168	ideal texture in the food industry	1156:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	7	16	theme	disulfide	990:998	arg1	bonds					1000:1004	protein disulfide bonds	982:1004	protein disulfide bonds	982:1004	Too high a concentration of guar gum hinders the formation of protein disulfide bonds.
35267367	0	17	theme	Gel	102:104	arg1	Microstructure					72:85	Microstructure	72:85	Microstructure	72:85	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	0	17	theme	Gel	102:104	arg1	Properties					57:66	Rheological Properties	45:66	Rheological Properties	45:66	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	8	18	theme	food	1086:1089	arg1	products					1091:1098	new food products	1082:1098	new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry	1082:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	8	19	theme	theoretical	1035:1045	arg1	basis					1047:1051	a theoretical basis	1033:1051	a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry	1033:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	2	20	theme	gel	416:418	arg1	microstructure					357:370	microstructure	357:370	microstructure	357:370	The linear and nonlinear rheological properties and microstructure of the phycocyanin-polysaccharide composite gel were evaluated.
35267367	2	20	theme	gel	416:418	arg1	properties					342:351	rheological properties	330:351	rheological properties	330:351	The linear and nonlinear rheological properties and microstructure of the phycocyanin-polysaccharide composite gel were evaluated.
35267367	6	21	theme	disulfide	822:830	arg1	bonds					832:836	disulfide bonds	822:836	disulfide bonds	822:836	As the concentration of κ-carrageenan increases, hydrophobic interactions and disulfide bonds play an essential role in maintaining the three-dimensional structure of the gel.
35267367	2	22	theme	linear	309:314	arg1	properties					342:351	rheological properties	330:351	rheological properties	330:351	The linear and nonlinear rheological properties and microstructure of the phycocyanin-polysaccharide composite gel were evaluated.
35267367	2	23	theme	composite	406:414	arg1	gel					416:418	the phycocyanin-polysaccharide composite gel	375:418	the phycocyanin-polysaccharide composite gel	375:418	The linear and nonlinear rheological properties and microstructure of the phycocyanin-polysaccharide composite gel were evaluated.
35267367	1	24	dep	performance	149:159	arg1	the					145:147	the	145:147	the	145:147	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	2	25	theme	phycocyanin-polysaccharide	379:404	arg1	gel					416:418	the phycocyanin-polysaccharide composite gel	375:418	the phycocyanin-polysaccharide composite gel	375:418	The linear and nonlinear rheological properties and microstructure of the phycocyanin-polysaccharide composite gel were evaluated.
35267367	1	26	from	effects	111:117	arg1	microstructure					165:178	microstructure	165:178	microstructure	165:178	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	1	26	from	effects	111:117	arg1	performance					149:159	performance	149:159	performance	149:159	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	0	27	dep	Properties	57:66	arg1	the					41:43	the	41:43	the	41:43	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	1	28	theme	gels	195:198	arg1	microstructure					165:178	microstructure	165:178	microstructure	165:178	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	1	28	theme	gels	195:198	arg1	performance					149:159	performance	149:159	performance	149:159	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	0	29	theme	κ-Carrageenan	11:23	arg1	Effects					0:6	Effects	0:6	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel	0:104	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	1	30	theme	guar	294:297	arg1	polysaccharides					277:291	neutral polysaccharides	269:291	neutral polysaccharides (guar gum)	269:302	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	1	30	theme	guar	294:297	arg1	gum					299:301	guar gum	294:301	guar gum	294:301	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	5	31	theme	elastic	726:732	arg1	behavior					734:741	mainly elastic behavior	719:741	mainly elastic behavior	719:741	All samples exhibited Type I behavior (inter-cycling strain-thinning) and mainly elastic behavior.
35267367	6	32	theme	gel	915:917	arg1	structure					898:906	the three-dimensional structure	876:906	the three-dimensional structure of the gel	876:917	As the concentration of κ-carrageenan increases, hydrophobic interactions and disulfide bonds play an essential role in maintaining the three-dimensional structure of the gel.
35267367	3	33	theme	gel	539:541	arg1	structure					514:522	the network structure	502:522	the network structure of phycocyanin gel	502:541	The results show that both κ-carrageenan and guar gum can enhance the network structure of phycocyanin gel and weaken the frequency dependence.
35267367	0	34	theme	Gum	34:36	arg1	Effects					0:6	Effects	0:6	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel	0:104	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	8	35	theme	ideal	1156:1160	arg1	texture					1162:1168	ideal texture	1156:1168	ideal texture in the food industry	1156:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	1	36	theme	phycocyanin	183:193	arg1	gels					195:198	phycocyanin gels	183:198	phycocyanin gels	183:198	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	0	37	theme	Guar	29:32	arg1	Gum					34:36	Guar Gum	29:36	Guar Gum	29:36	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	8	38	theme	different	1125:1133	arg1	polysaccharides					1135:1149	different polysaccharides	1125:1149	different polysaccharides with ideal texture in the food industry	1125:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	8	39	from	texture	1162:1168	arg1	industry					1182:1189	the food industry	1173:1189	the food industry	1173:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	4	40	contain	has	615:617	arg2	strength					635:642	the highest gel strength	619:642	the highest gel strength	619:642	The sample with 0.4% κ-carrageenan has the highest gel strength.
35267367	4	40	contain	has	615:617	arg1	sample					584:589	The sample	580:589	The sample with 0.4% κ-carrageenan	580:613	The sample with 0.4% κ-carrageenan has the highest gel strength.
35267367	6	41	theme	hydrophobic	793:803	arg1	interactions					805:816	hydrophobic interactions	793:816	hydrophobic interactions	793:816	As the concentration of κ-carrageenan increases, hydrophobic interactions and disulfide bonds play an essential role in maintaining the three-dimensional structure of the gel.
35267367	3	42	theme	guar	481:484	arg1	gum					486:488	guar gum	481:488	guar gum	481:488	The results show that both κ-carrageenan and guar gum can enhance the network structure of phycocyanin gel and weaken the frequency dependence.
35267367	5	43	theme	inter-cycling	684:696	arg1	behavior					674:681	Type I behavior	667:681	Type I behavior (inter-cycling strain-thinning)	667:713	All samples exhibited Type I behavior (inter-cycling strain-thinning) and mainly elastic behavior.
35267367	5	43	theme	inter-cycling	684:696	arg1	strain-thinning					698:712	inter-cycling strain-thinning	684:712	inter-cycling strain-thinning	684:712	All samples exhibited Type I behavior (inter-cycling strain-thinning) and mainly elastic behavior.
35267367	3	44	theme	network	506:512	arg1	structure					514:522	the network structure	502:522	the network structure of phycocyanin gel	502:541	The results show that both κ-carrageenan and guar gum can enhance the network structure of phycocyanin gel and weaken the frequency dependence.
35267367	6	45	theme	three-dimensional	880:896	arg1	structure					898:906	the three-dimensional structure	876:906	the three-dimensional structure of the gel	876:917	As the concentration of κ-carrageenan increases, hydrophobic interactions and disulfide bonds play an essential role in maintaining the three-dimensional structure of the gel.
35267367	2	46	theme	rheological	330:340	arg1	properties					342:351	rheological properties	330:351	rheological properties	330:351	The linear and nonlinear rheological properties and microstructure of the phycocyanin-polysaccharide composite gel were evaluated.
35267367	7	47	theme	guar	948:951	arg1	gum					953:955	guar gum	948:955	guar gum	948:955	Too high a concentration of guar gum hinders the formation of protein disulfide bonds.
35267367	6	48	theme	κ-carrageenan	768:780	arg1	concentration					751:763	the concentration	747:763	the concentration of κ-carrageenan	747:780	As the concentration of κ-carrageenan increases, hydrophobic interactions and disulfide bonds play an essential role in maintaining the three-dimensional structure of the gel.
35267367	2	49	theme	nonlinear	320:328	arg1	properties					342:351	rheological properties	330:351	rheological properties	330:351	The linear and nonlinear rheological properties and microstructure of the phycocyanin-polysaccharide composite gel were evaluated.
35267367	7	50	theme	gum	953:955	arg1	concentration					931:943	Too high a concentration	920:943	Too high a concentration of guar gum	920:955	Too high a concentration of guar gum hinders the formation of protein disulfide bonds.
35267367	4	51	theme	highest	623:629	arg1	strength					635:642	the highest gel strength	619:642	the highest gel strength	619:642	The sample with 0.4% κ-carrageenan has the highest gel strength.
35267367	1	52	theme	anionic	225:231	arg1	polysaccharides					233:247	anionic polysaccharides	225:247	anionic polysaccharides (κ-carrageenan)	225:263	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	1	52	theme	anionic	225:231	arg1	κ-carrageenan					250:262	κ-carrageenan	250:262	κ-carrageenan	250:262	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	8	53	theme	food	1177:1180	arg1	industry					1182:1189	the food industry	1173:1189	the food industry	1173:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	1	54	theme	polysaccharides	126:140	arg1	effects					111:117	The effects	107:117	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels	107:198	The effects of two polysaccharides on the performance and microstructure of phycocyanin gels were studied by choosing anionic polysaccharides (κ-carrageenan) and neutral polysaccharides (guar gum).
35267367	8	55	with	phycocyanin	1109:1119	arg1	texture					1162:1168	ideal texture	1156:1168	ideal texture in the food industry	1156:1189	This research can provide a theoretical basis for designing and developing new food products based on phycocyanin and different polysaccharides with ideal texture in the food industry.
35267367	0	56	theme	Rheological	45:55	arg1	Properties					57:66	Rheological Properties	45:66	Rheological Properties	45:66	Effects of κ-Carrageenan and Guar Gum on the Rheological Properties and Microstructure of Phycocyanin Gel.
35267367	7	57	theme	high	924:927	arg1	concentration					931:943	Too high a concentration	920:943	Too high a concentration of guar gum	920:955	Too high a concentration of guar gum hinders the formation of protein disulfide bonds.
35267367	4	58	theme	gel	631:633	arg1	strength					635:642	the highest gel strength	619:642	the highest gel strength	619:642	The sample with 0.4% κ-carrageenan has the highest gel strength.
36192796	11	0	theme	gene	1654:1657	arg1	data					1670:1673	16S rRNA gene sequencing data	1645:1673	16S rRNA gene sequencing data	1645:1673	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	2	1	theme	Poria	328:332	arg1	PCP					357:359	PCP	357:359	PCP	357:359	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	1	theme	Poria	328:332	arg1	polysaccharides					340:354	Poria cocos polysaccharides	328:354	Poria cocos polysaccharides (PCP)	328:360	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	1	theme	Poria	328:332	arg1	medicine					392:399	a traditional Chinese herbal medicine	363:399	a traditional Chinese herbal medicine with various bioactivities and prebiotic effects	363:448	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	6	2	theme	junction	880:887	arg1	molecules					889:897	gut epithelial junction molecules	865:897	gut epithelial junction molecules	865:897	Expressions of mucosal cytokines and gut epithelial junction molecules were measured using qRT-PCR.
36192796	2	3	theme	gut	571:573	arg1	microflora					575:584	the commensal gut microflora	557:584	the commensal gut microflora	557:584	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	10	4	theme	PCP	1358:1360	arg1	treatment					1362:1370	PCP treatment	1358:1370	PCP treatment	1358:1370	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	13	5	theme	L.johnsonii	2173:2183	arg1	transplantation					2154:2168	transplantation	2154:2168	transplantation of L.johnsonii and B.animalis	2154:2198	Moreover, the results showed that transplantation of L.johnsonii and B.animalis effectively reduced the polyp burden in ApcMin/+ mice being treated with 5-FU.
36192796	8	6	theme	PCP	1160:1162	arg1	effects					1149:1155	the prebiotic effects	1135:1155	the prebiotic effects of PCP in improving the efficacy of 5-FU	1135:1196	Transplantation of Lactobacillus johnsonii and Bifidobacterium animalis were performed to verify the prebiotic effects of PCP in improving the efficacy of 5-FU.
36192796	6	7	theme	gut	865:867	arg1	molecules					889:897	gut epithelial junction molecules	865:897	gut epithelial junction molecules	865:897	Expressions of mucosal cytokines and gut epithelial junction molecules were measured using qRT-PCR.
36192796	11	8	theme	sequencing	1659:1668	arg1	data					1670:1673	16S rRNA gene sequencing data	1645:1673	16S rRNA gene sequencing data	1645:1673	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	1	9	theme	first-line	164:173	arg1	5-fluorouracil					199:212	5-fluorouracil	199:212	5-fluorouracil (5-FU)	199:219	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36192796	1	9	theme	first-line	164:173	arg1	agent					192:196	a first-line chemotherapeutic agent	162:196	a first-line chemotherapeutic agent	162:196	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36192796	6	10	theme	cytokines	851:859	arg1	Expressions					828:838	Expressions	828:838	Expressions of mucosal cytokines and gut epithelial junction molecules	828:897	Expressions of mucosal cytokines and gut epithelial junction molecules were measured using qRT-PCR.
36192796	10	11	theme	cytotoxic	1382:1390	arg1	effects					1392:1398	the cytotoxic effects	1378:1398	the cytotoxic effects of 5-FU	1378:1406	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	10	12	theme	junction	1579:1586	arg1	proteins					1588:1595	the tight junction proteins	1569:1595	the tight junction proteins	1569:1595	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	5	13	theme	PCP	814:816	arg1	effect					804:809	the anti-cancer effect	788:809	the anti-cancer effect of PCP and 5-FU	788:825	Polyp count was used to assess the anti-cancer effect of PCP and 5-FU.
36192796	8	14	theme	5-FU	1193:1196	arg1	efficacy					1181:1188	the efficacy	1177:1188	the efficacy of 5-FU	1177:1196	Transplantation of Lactobacillus johnsonii and Bifidobacterium animalis were performed to verify the prebiotic effects of PCP in improving the efficacy of 5-FU.
36192796	5	15	theme	5-FU	822:825	arg1	effect					804:809	the anti-cancer effect	788:809	the anti-cancer effect of PCP and 5-FU	788:825	Polyp count was used to assess the anti-cancer effect of PCP and 5-FU.
36192796	12	16	theme	pathogens	1962:1970	arg1	growth					1942:1947	the growth	1938:1947	the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans)	1938:2117	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	10	17	theme	adhesion	1612:1619	arg1	molecules					1621:1629	associated adhesion molecules	1601:1629	associated adhesion molecules	1601:1629	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	7	18	theme	microbiota	1021:1030	arg1	changes					1006:1012	the compositional changes	988:1012	the compositional changes of gut microbiota (GM)	988:1035	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	9	19	theme	PCP	1231:1233	arg1	treatment					1235:1243	PCP treatment	1231:1243	PCP treatment	1231:1243	RESULTS The results showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice.
36192796	2	20	theme	microenvironment	536:551	arg1	microflora					575:584	the commensal gut microflora	557:584	the commensal gut microflora	557:584	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	20	theme	microenvironment	536:551	arg1	homeostasis					513:523	the homeostasis	509:523	the homeostasis of the gut microenvironment	509:551	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	12	21	theme	Desulfovibrio	2090:2102	arg1	desulfuricans					2104:2116	Desulfovibrio desulfuricans	2090:2116	Desulfovibrio desulfuricans	2090:2116	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	11	22	theme	probiotic	1740:1748	arg1	bacteria					1750:1757	probiotic bacteria	1740:1757	probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium)	1740:1904	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	9	23	theme	weight	1260:1265	arg1	loss					1267:1270	the weight loss	1256:1270	the weight loss caused by 5-FU treatment	1256:1295	RESULTS The results showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice.
36192796	3	24	theme	ApcMin/+	595:602	arg1	mice					604:607	ApcMin/+ mice	595:607	ApcMin/+ mice	595:607	METHODS ApcMin/+ mice were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment.
36192796	7	25	theme	fecal	956:960	arg1	DNAs					962:965	fecal DNAs	956:965	fecal DNAs	956:965	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	12	26	dep	pathogens	1962:1970	arg1	Citrobacter					2048:2058	Citrobacter	2048:2058	Citrobacter	2048:2058	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	12	26	dep	pathogens	1962:1970	arg1	e.g.					1973:1976	e.g.	1973:1976	e.g.	1973:1976	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	12	26	dep	pathogens	1962:1970	arg1	desulfuricans					2104:2116	Desulfovibrio desulfuricans	2090:2116	Desulfovibrio desulfuricans	2090:2116	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	12	26	dep	pathogens	1962:1970	arg1	Alistipes					2025:2033	Alistipes	2025:2033	Alistipes	2025:2033	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	12	26	dep	pathogens	1962:1970	arg1	putredinis.					2035:2045	putredinis.	2035:2045	putredinis.	2035:2045	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	12	26	dep	pathogens	1962:1970	arg1	spp.					2080:2083	Desulfovibrio spp.	2066:2083	Desulfovibrio spp.	2066:2083	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	12	26	dep	pathogens	1962:1970	arg1	finegoldii					1989:1998	Alistipes finegoldii	1979:1998	Alistipes finegoldii	1979:1998	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	12	27	theme	Desulfovibrio	2066:2078	arg1	spp.					2080:2083	Desulfovibrio spp.	2066:2083	Desulfovibrio spp.	2066:2083	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	5	28	theme	Polyp	757:761	arg1	count					763:767	Polyp count	757:767	Polyp count	757:767	Polyp count was used to assess the anti-cancer effect of PCP and 5-FU.
36192796	7	29	used	used	971:974	arg2	sequencing					942:951	16S rRNA gene sequencing	928:951	16S rRNA gene sequencing of fecal DNAs	928:965	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	9	30	theme	5-FU	1282:1285	arg1	treatment					1287:1295	5-FU treatment	1282:1295	5-FU treatment	1282:1295	RESULTS The results showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice.
36192796	7	31	theme	rRNA	932:935	arg1	sequencing					942:951	16S rRNA gene sequencing	928:951	16S rRNA gene sequencing of fecal DNAs	928:965	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	2	32	theme	5-FU	491:494	arg1	effect					481:486	the therapeutic effect	465:486	the therapeutic effect of 5-FU	465:494	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	10	33	theme	cytokines	1456:1464	arg1	expressions					1424:1434	the expressions	1420:1434	the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines	1420:1508	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	0	34	theme	therapeutic	102:112	arg1	outcome					114:120	the therapeutic outcome	98:120	the therapeutic outcome of 5-FU in ApcMin/+ mice	98:145	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	12	35	dep	Citrobacter	2048:2058	arg1	spp.					2060:2063	Citrobacter spp.	2048:2063	Citrobacter spp.	2048:2063	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	14	36	theme	gut	2499:2501	arg1	microbiota					2503:2512	the gut microbiota	2495:2512	the gut microbiota of ApcMin/+ mice	2495:2529	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	4	37	theme	food	717:720	arg1	consumption					722:732	food consumption	717:732	food consumption	717:732	Body weight and food consumption were monitored weekly.
36192796	0	38	theme	Poria	0:4	arg1	polysaccharides					12:26	Poria cocos polysaccharides	0:26	Poria cocos polysaccharides	0:26	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	13	39	theme	ApcMin/+	2240:2247	arg1	mice					2249:2252	ApcMin/+ mice	2240:2252	ApcMin/+ mice	2240:2252	Moreover, the results showed that transplantation of L.johnsonii and B.animalis effectively reduced the polyp burden in ApcMin/+ mice being treated with 5-FU.
36192796	12	40	theme	same	1914:1917	arg1	time					1919:1922	the same time	1910:1922	the same time	1910:1922	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	2	41	theme	various	406:412	arg1	bioactivities					414:426	various bioactivities	406:426	various bioactivities	406:426	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	10	42	theme	anti-inflammatory	1482:1498	arg1	cytokines					1500:1508	the anti-inflammatory cytokines	1478:1508	the anti-inflammatory cytokines	1478:1508	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	1	43	theme	cancer	275:280	arg1	treatment					282:290	cancer treatment	275:290	cancer treatment	275:290	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36192796	5	44	used	used	773:776	arg2	count					763:767	Polyp count	757:767	Polyp count	757:767	Polyp count was used to assess the anti-cancer effect of PCP and 5-FU.
36192796	6	45	theme	mucosal	843:849	arg1	cytokines					851:859	mucosal cytokines	843:859	mucosal cytokines	843:859	Expressions of mucosal cytokines and gut epithelial junction molecules were measured using qRT-PCR.
36192796	2	46	theme	Chinese	377:383	arg1	polysaccharides					340:354	Poria cocos polysaccharides	328:354	Poria cocos polysaccharides (PCP)	328:360	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	46	theme	Chinese	377:383	arg1	medicine					392:399	a traditional Chinese herbal medicine	363:399	a traditional Chinese herbal medicine with various bioactivities and prebiotic effects	363:448	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	0	47	theme	prebiotic	34:42	arg1	function					44:51	prebiotic function	34:51	prebiotic function	34:51	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	9	48	theme	ApcMin/+	1329:1336	arg1	mice					1338:1341	ApcMin/+ mice	1329:1341	ApcMin/+ mice	1329:1341	RESULTS The results showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice.
36192796	14	49	theme	epithelial	2460:2469	arg1	barrier					2471:2477	the gut epithelial barrier	2452:2477	the gut epithelial barrier	2452:2477	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	3	50	theme	5-FU	661:664	arg1	effect					651:656	the anti-cancer effect	635:656	the anti-cancer effect of 5-FU in conjunction with PCP treatment	635:698	METHODS ApcMin/+ mice were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment.
36192796	11	51	theme	genera	1854:1859	arg1	Lactobacillus					1861:1873	the genera Lactobacillus	1850:1873	the genera Lactobacillus	1850:1873	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	8	52	theme	animalis	1101:1108	arg1	Transplantation					1038:1052	Transplantation	1038:1052	Transplantation of Lactobacillus johnsonii and Bifidobacterium animalis	1038:1108	Transplantation of Lactobacillus johnsonii and Bifidobacterium animalis were performed to verify the prebiotic effects of PCP in improving the efficacy of 5-FU.
36192796	1	53	theme	many	230:233	arg1	effects					240:246	many side effects	230:246	many side effects	230:246	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36192796	14	54	theme	ApcMin/+	2517:2524	arg1	mice					2526:2529	ApcMin/+ mice	2517:2529	ApcMin/+ mice	2517:2529	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	3	55	theme	PCP	686:688	arg1	treatment					690:698	PCP treatment	686:698	PCP treatment	686:698	METHODS ApcMin/+ mice were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment.
36192796	14	56	theme	intestinal	2417:2426	arg1	inflammation					2428:2439	intestinal inflammation	2417:2439	intestinal inflammation	2417:2439	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	2	57	theme	cocos	334:338	arg1	PCP					357:359	PCP	357:359	PCP	357:359	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	57	theme	cocos	334:338	arg1	polysaccharides					340:354	Poria cocos polysaccharides	328:354	Poria cocos polysaccharides (PCP)	328:360	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	57	theme	cocos	334:338	arg1	medicine					392:399	a traditional Chinese herbal medicine	363:399	a traditional Chinese herbal medicine with various bioactivities and prebiotic effects	363:448	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	3	58	with	conjunction	669:679	arg1	treatment					690:698	PCP treatment	686:698	PCP treatment	686:698	METHODS ApcMin/+ mice were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment.
36192796	6	59	theme	molecules	889:897	arg1	Expressions					828:838	Expressions	828:838	Expressions of mucosal cytokines and gut epithelial junction molecules	828:897	Expressions of mucosal cytokines and gut epithelial junction molecules were measured using qRT-PCR.
36192796	13	60	from	burden	2230:2235	arg1	mice					2249:2252	ApcMin/+ mice	2240:2252	ApcMin/+ mice	2240:2252	Moreover, the results showed that transplantation of L.johnsonii and B.animalis effectively reduced the polyp burden in ApcMin/+ mice being treated with 5-FU.
36192796	10	61	theme	tight	1573:1577	arg1	proteins					1588:1595	the tight junction proteins	1569:1595	the tight junction proteins	1569:1595	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	11	62	theme	16S	1645:1647	arg1	rRNA					1649:1652	16S rRNA	1645:1652	16S rRNA gene sequencing data	1645:1673	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	8	63	theme	prebiotic	1139:1147	arg1	effects					1149:1155	the prebiotic effects	1135:1155	the prebiotic effects of PCP in improving the efficacy of 5-FU	1135:1196	Transplantation of Lactobacillus johnsonii and Bifidobacterium animalis were performed to verify the prebiotic effects of PCP in improving the efficacy of 5-FU.
36192796	6	64	theme	epithelial	869:878	arg1	molecules					889:897	gut epithelial junction molecules	865:897	gut epithelial junction molecules	865:897	Expressions of mucosal cytokines and gut epithelial junction molecules were measured using qRT-PCR.
36192796	2	65	theme	commensal	561:569	arg1	microflora					575:584	the commensal gut microflora	557:584	the commensal gut microflora	557:584	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	0	66	from	mice	142:145	arg1	outcome					114:120	the therapeutic outcome	98:120	the therapeutic outcome of 5-FU in ApcMin/+ mice	98:145	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	14	67	theme	side	2392:2395	arg1	effects					2397:2403	its side effects	2388:2403	its side effects	2388:2403	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	2	68	with	medicine	392:399	arg1	bioactivities					414:426	various bioactivities	406:426	various bioactivities	406:426	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	68	with	medicine	392:399	arg1	effects					442:448	prebiotic effects	432:448	prebiotic effects	432:448	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	14	69	dep	CONCLUSION	2279:2288	arg1	showed					2300:2305	showed	2300:2305	showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice	2300:2529	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	10	70	theme	associated	1601:1610	arg1	molecules					1621:1629	associated adhesion molecules	1601:1629	associated adhesion molecules	1601:1629	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	1	71	from	efficacy	263:270	arg1	treatment					282:290	cancer treatment	275:290	cancer treatment	275:290	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36192796	1	72	theme	chemotherapeutic	175:190	arg1	5-fluorouracil					199:212	5-fluorouracil	199:212	5-fluorouracil (5-FU)	199:219	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36192796	1	72	theme	chemotherapeutic	175:190	arg1	agent					192:196	a first-line chemotherapeutic agent	162:196	a first-line chemotherapeutic agent	162:196	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36192796	0	73	theme	5-FU	125:128	arg1	outcome					114:120	the therapeutic outcome	98:120	the therapeutic outcome of 5-FU in ApcMin/+ mice	98:145	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	14	74	theme	5-FU	2368:2371	arg1	effect					2358:2363	the anti-cancer effect	2342:2363	the anti-cancer effect of 5-FU	2342:2371	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	3	75	dep	METHODS	587:593	arg1	employed					614:621	employed	614:621	were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment	609:698	METHODS ApcMin/+ mice were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment.
36192796	0	76	theme	ApcMin/+	133:140	arg1	mice					142:145	ApcMin/+ mice	133:145	ApcMin/+ mice	133:145	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	7	77	theme	gut	1017:1019	arg1	GM					1033:1034	GM	1033:1034	GM	1033:1034	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	7	77	theme	gut	1017:1019	arg1	microbiota					1021:1030	gut microbiota	1017:1030	gut microbiota (GM)	1017:1035	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	11	78	dep	bacteria	1750:1757	arg1	Lactobacillus					1861:1873	the genera Lactobacillus	1850:1873	the genera Lactobacillus	1850:1873	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	11	78	dep	bacteria	1750:1757	arg1	Bifidobacterium					1876:1890	Bifidobacterium	1876:1890	Bifidobacterium	1876:1890	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	11	78	dep	bacteria	1750:1757	arg1	acidifaciens					1772:1783	Bacteroides acidifaciens	1760:1783	Bacteroides acidifaciens	1760:1783	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	11	78	dep	bacteria	1750:1757	arg1	pullicaecorum					1831:1843	Butyricicoccus pullicaecorum	1816:1843	Butyricicoccus pullicaecorum	1816:1843	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	11	78	dep	bacteria	1750:1757	arg1	Eubacterium					1893:1903	Eubacterium	1893:1903	Eubacterium	1893:1903	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	11	78	dep	bacteria	1750:1757	arg1	intestinihominis					1798:1813	Bacteroides intestinihominis	1786:1813	Bacteroides intestinihominis	1786:1813	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	7	79	theme	compositional	992:1004	arg1	changes					1006:1012	the compositional changes	988:1012	the compositional changes of gut microbiota (GM)	988:1035	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	12	80	theme	potential	1952:1960	arg1	pathogens					1962:1970	potential pathogens	1952:1970	potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans)	1952:2117	At the same time, it inhibited the growth of potential pathogens (e.g., Alistipes finegoldii, Alistipes massiliensis, Alistipes putredinis., Citrobacter spp., Desulfovibrio spp., and Desulfovibrio desulfuricans).
36192796	0	81	from	outcome	114:120	arg1	mice					142:145	ApcMin/+ mice	133:145	ApcMin/+ mice	133:145	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	2	82	theme	gut	532:534	arg1	microenvironment					536:551	the gut microenvironment	528:551	the gut microenvironment	528:551	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	4	83	theme	Body	701:704	arg1	weight					706:711	Body weight	701:711	Body weight	701:711	Body weight and food consumption were monitored weekly.
36192796	14	84	theme	anti-cancer	2346:2356	arg1	effect					2358:2363	the anti-cancer effect	2342:2363	the anti-cancer effect of 5-FU	2342:2371	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	11	85	theme	bacteria	1750:1757	arg1	growth					1730:1735	the growth	1726:1735	the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium)	1726:1904	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	0	86	theme	adverse	70:76	arg1	effects					78:84	the adverse effects	66:84	the adverse effects	66:84	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	2	87	theme	therapeutic	469:479	arg1	effect					481:486	the therapeutic effect	465:486	the therapeutic effect of 5-FU	465:494	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	7	88	theme	DNAs	962:965	arg1	sequencing					942:951	16S rRNA gene sequencing	928:951	16S rRNA gene sequencing of fecal DNAs	928:965	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	9	89	dep	RESULTS	1199:1205	arg1	showed					1219:1224	showed	1219:1224	showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice	1219:1341	RESULTS The results showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice.
36192796	10	90	theme	5-FU	1403:1406	arg1	effects					1392:1398	the cytotoxic effects	1378:1398	the cytotoxic effects of 5-FU	1378:1406	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	9	91	from	burden	1319:1324	arg1	mice					1338:1341	ApcMin/+ mice	1329:1341	ApcMin/+ mice	1329:1341	RESULTS The results showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice.
36192796	7	92	theme	gene	937:940	arg1	sequencing					942:951	16S rRNA gene sequencing	928:951	16S rRNA gene sequencing of fecal DNAs	928:965	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	10	93	theme	pro-inflammatory	1439:1454	arg1	cytokines					1456:1464	pro-inflammatory cytokines	1439:1464	pro-inflammatory cytokines	1439:1464	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	0	94	theme	cocos	6:10	arg1	polysaccharides					12:26	Poria cocos polysaccharides	0:26	Poria cocos polysaccharides	0:26	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	7	95	theme	16S	928:930	arg1	sequencing					942:951	16S rRNA gene sequencing	928:951	16S rRNA gene sequencing of fecal DNAs	928:965	16S rRNA gene sequencing of fecal DNAs was used to evaluate the compositional changes of gut microbiota (GM).
36192796	5	96	theme	anti-cancer	792:802	arg1	effect					804:809	the anti-cancer effect	788:809	the anti-cancer effect of PCP and 5-FU	788:825	Polyp count was used to assess the anti-cancer effect of PCP and 5-FU.
36192796	9	97	theme	polyp	1313:1317	arg1	burden					1319:1324	the polyp burden	1309:1324	the polyp burden in ApcMin/+ mice	1309:1341	RESULTS The results showed that PCP treatment alleviated the weight loss caused by 5-FU treatment and reduced the polyp burden in ApcMin/+ mice.
36192796	2	98	theme	herbal	385:390	arg1	polysaccharides					340:354	Poria cocos polysaccharides	328:354	Poria cocos polysaccharides (PCP)	328:360	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	98	theme	herbal	385:390	arg1	medicine					392:399	a traditional Chinese herbal medicine	363:399	a traditional Chinese herbal medicine with various bioactivities and prebiotic effects	363:448	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	8	99	theme	johnsonii	1071:1079	arg1	Transplantation					1038:1052	Transplantation	1038:1052	Transplantation of Lactobacillus johnsonii and Bifidobacterium animalis	1038:1108	Transplantation of Lactobacillus johnsonii and Bifidobacterium animalis were performed to verify the prebiotic effects of PCP in improving the efficacy of 5-FU.
36192796	2	100	theme	traditional	365:375	arg1	polysaccharides					340:354	Poria cocos polysaccharides	328:354	Poria cocos polysaccharides (PCP)	328:360	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	2	100	theme	traditional	365:375	arg1	medicine					392:399	a traditional Chinese herbal medicine	363:399	a traditional Chinese herbal medicine with various bioactivities and prebiotic effects	363:448	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	13	101	theme	polyp	2224:2228	arg1	burden					2230:2235	the polyp burden	2220:2235	the polyp burden in ApcMin/+ mice	2220:2252	Moreover, the results showed that transplantation of L.johnsonii and B.animalis effectively reduced the polyp burden in ApcMin/+ mice being treated with 5-FU.
36192796	3	102	from	effect	651:656	arg1	conjunction					669:679	conjunction	669:679	conjunction with PCP treatment	669:698	METHODS ApcMin/+ mice were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment.
36192796	2	103	theme	prebiotic	432:440	arg1	effects					442:448	prebiotic effects	432:448	prebiotic effects	432:448	In this study, we hypothesize that Poria cocos polysaccharides (PCP), a traditional Chinese herbal medicine with various bioactivities and prebiotic effects, might improve the therapeutic effect of 5-FU by restoring the homeostasis of the gut microenvironment and the commensal gut microflora.
36192796	14	104	theme	gut	2456:2458	arg1	barrier					2471:2477	the gut epithelial barrier	2452:2477	the gut epithelial barrier	2452:2477	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	3	105	theme	anti-cancer	639:649	arg1	effect					651:656	the anti-cancer effect	635:656	the anti-cancer effect of 5-FU in conjunction with PCP treatment	635:698	METHODS ApcMin/+ mice were employed to evaluate the anti-cancer effect of 5-FU in conjunction with PCP treatment.
36192796	13	106	theme	B.animalis	2189:2198	arg1	transplantation					2154:2168	transplantation	2154:2168	transplantation of L.johnsonii and B.animalis	2154:2198	Moreover, the results showed that transplantation of L.johnsonii and B.animalis effectively reduced the polyp burden in ApcMin/+ mice being treated with 5-FU.
36192796	14	107	theme	mice	2526:2529	arg1	microbiota					2503:2512	the gut microbiota	2495:2512	the gut microbiota of ApcMin/+ mice	2495:2529	CONCLUSION Our study showed that PCP could effectively improve the anti-cancer effect of 5-FU by attenuating its side effects, modulating intestinal inflammation, improving the gut epithelial barrier, and modulating the gut microbiota of ApcMin/+ mice.
36192796	0	108	from	5-FU	125:128	arg1	mice					142:145	ApcMin/+ mice	133:145	ApcMin/+ mice	133:145	Poria cocos polysaccharides exert prebiotic function to attenuate the adverse effects and improve the therapeutic outcome of 5-FU in ApcMin/+ mice.
36192796	10	109	theme	gut	1543:1545	arg1	barriers					1547:1554	the gut barriers	1539:1554	the gut barriers	1539:1554	Additionally, PCP treatment eased the cytotoxic effects of 5-FU by reducing the expressions of pro-inflammatory cytokines, increasing the anti-inflammatory cytokines; and significantly improving the gut barriers by enhancing the tight junction proteins and associated adhesion molecules.
36192796	11	110	theme	rRNA	1649:1652	arg1	data					1670:1673	16S rRNA gene sequencing data	1645:1673	16S rRNA gene sequencing data	1645:1673	Furthermore, 16S rRNA gene sequencing data showed that PCP alone or with 5-FU could stimulate the growth of probiotic bacteria (Bacteroides acidifaciens, Bacteroides intestinihominis, Butyricicoccus pullicaecorum, and the genera Lactobacillus, Bifidobacterium, Eubacterium).
36192796	1	111	theme	side	235:238	arg1	effects					240:246	many side effects	230:246	many side effects	230:246	BACKGROUND As a first-line chemotherapeutic agent, 5-fluorouracil (5-FU) exhibits many side effects, weakening its efficacy in cancer treatment.
36375605	8	0	from	crucial	1366:1372	arg1	homo-aggregation					1381:1396	the homo-aggregation	1377:1396	the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa	1377:1465	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	6	1	theme	shading	848:854	arg1	effect					856:861	the shading effect	844:861	the shading effect	844:861	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	5	2	dep	M.	757:758	arg1	aeruginosa					760:769	aeruginosa	760:769	aeruginosa	760:769	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	0	3	theme	different	81:89	arg1	sizes					91:95	different sizes	81:95	different sizes of polystyrene microplastics	81:124	Roles of extracellular polymeric substances on Microcystis aeruginosa exposed to different sizes of polystyrene microplastics.
36375605	8	4	theme	adverse	1434:1440	arg1	effects					1442:1448	its adverse effects	1430:1448	its adverse effects on M. aeruginosa	1430:1465	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	7	5	theme	representative	1038:1051	arg1	proteins					1008:1015	aromatic proteins	999:1015	aromatic proteins	999:1015	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	5	theme	representative	1038:1051	arg1	acids					1028:1032	fulvic acids	1021:1032	fulvic acids	1021:1032	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	5	theme	representative	1038:1051	arg1	components					1057:1066	representative EPS components	1038:1066	representative EPS components stimulated by 100 nm PS MPs	1038:1094	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	1	6	theme	important	172:180	arg1	substances					151:160	Extracellular polymeric substances	127:160	Extracellular polymeric substances (EPS)	127:166	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	1	6	theme	important	172:180	arg1	shields					182:188	important shields	172:188	important shields for microalgae	172:203	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	0	7	theme	polystyrene	100:110	arg1	microplastics					112:124	polystyrene microplastics	100:124	polystyrene microplastics	100:124	Roles of extracellular polymeric substances on Microcystis aeruginosa exposed to different sizes of polystyrene microplastics.
36375605	0	8	from	Roles	0:4	arg1	aeruginosa					59:68	Microcystis aeruginosa	47:68	Microcystis aeruginosa	47:68	Roles of extracellular polymeric substances on Microcystis aeruginosa exposed to different sizes of polystyrene microplastics.
36375605	7	9	theme	fulvic	1021:1026	arg1	proteins					1008:1015	aromatic proteins	999:1015	aromatic proteins	999:1015	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	9	theme	fulvic	1021:1026	arg1	acids					1028:1032	fulvic acids	1021:1032	fulvic acids	1021:1032	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	9	theme	fulvic	1021:1026	arg1	components					1057:1066	representative EPS components	1038:1066	representative EPS components stimulated by 100 nm PS MPs	1038:1094	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	5	10	from	effects	736:742	arg1	photosynthesis					784:797	photosynthesis	784:797	photosynthesis	784:797	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	5	10	from	effects	736:742	arg1	growth					776:781	M. aeruginosa cell growth	757:781	M. aeruginosa cell growth	757:781	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	5	10	from	effects	736:742	arg1	stress					814:819	oxidative stress	804:819	oxidative stress	804:819	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	6	11	theme	1 μm PS	917:923	arg1	MPs					925:927	unstable 1 μm PS MPs	908:927	unstable 1 μm PS MPs	908:927	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	5	12	theme	EPS	693:695	arg1	presence					681:688	The presence	677:688	The presence of EPS	677:695	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	5	12	theme	EPS	693:695	arg1	helpful					700:706	helpful	700:706	helpful	700:706	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	1	13	theme	Extracellular	127:139	arg1	substances					151:160	Extracellular polymeric substances	127:160	Extracellular polymeric substances (EPS)	127:166	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	1	13	theme	Extracellular	127:139	arg1	shields					182:188	important shields	172:188	important shields for microalgae	172:203	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	1	13	theme	Extracellular	127:139	arg1	EPS					163:165	EPS	163:165	EPS	163:165	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	0	14	theme	microplastics	112:124	arg1	sizes					91:95	different sizes	81:95	different sizes of polystyrene microplastics	81:124	Roles of extracellular polymeric substances on Microcystis aeruginosa exposed to different sizes of polystyrene microplastics.
36375605	8	15	from	homo-aggregation	1381:1396	arg1	crucial					1366:1372	crucial	1366:1372	crucial	1366:1372	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	3	16	theme	EPS	496:498	arg1	compositions					480:491	the compositions	476:491	the compositions	476:491	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	5	17	theme	MPs	750:752	arg1	effects					736:742	the adverse effects	724:742	the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress	724:819	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	6	18	theme	direct	887:892	arg1	adsorption					894:903	direct adsorption	887:903	direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall	887:983	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	9	19	theme	different	1560:1568	arg1	sizes					1570:1574	different sizes	1560:1574	different sizes	1560:1574	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	8	20	theme	humic	1303:1307	arg1	acids					1309:1313	humic acids	1303:1313	humic acids	1303:1313	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	5	21	theme	oxidative	804:812	arg1	stress					814:819	oxidative stress	804:819	oxidative stress	804:819	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	6	22	theme	unstable	908:915	arg1	MPs					925:927	unstable 1 μm PS MPs	908:927	unstable 1 μm PS MPs	908:927	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	7	23	theme	aromatic	999:1006	arg1	proteins					1008:1015	aromatic proteins	999:1015	aromatic proteins	999:1015	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	23	theme	aromatic	999:1006	arg1	acids					1028:1032	fulvic acids	1021:1032	fulvic acids	1021:1032	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	23	theme	aromatic	999:1006	arg1	components					1057:1066	representative EPS components	1038:1066	representative EPS components stimulated by 100 nm PS MPs	1038:1094	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	9	24	theme	PS	1664:1665	arg1	MPs					1667:1669	PS MPs	1664:1669	PS MPs	1664:1669	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	2	25	from	roles	270:274	arg1	interactions					290:301	the interactions	286:301	the interactions between microplastics (MPs) and microalgae	286:344	However, the underlying roles of EPS in the interactions between microplastics (MPs) and microalgae remain poorly understood.
36375605	3	26	theme	MPs	419:421	arg1	sizes					393:397	three sizes	387:397	three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm)	387:447	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	0	27	theme	polymeric	23:31	arg1	substances					33:42	extracellular polymeric substances	9:42	extracellular polymeric substances	9:42	Roles of extracellular polymeric substances on Microcystis aeruginosa exposed to different sizes of polystyrene microplastics.
36375605	8	28	from	effects	1442:1448	arg1	aeruginosa					1456:1465	M. aeruginosa	1453:1465	M. aeruginosa	1453:1465	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	8	29	theme	High	1229:1232	arg1	polysaccharides					1245:1259	polysaccharides	1245:1259	polysaccharides	1245:1259	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	8	29	theme	High	1229:1232	arg1	amounts					1234:1240	High amounts	1229:1240	High amounts of polysaccharides	1229:1259	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	5	30	theme	cell	771:774	arg1	growth					776:781	M. aeruginosa cell growth	757:781	M. aeruginosa cell growth	757:781	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	0	31	theme	extracellular	9:21	arg1	substances					33:42	extracellular polymeric substances	9:42	extracellular polymeric substances	9:42	Roles of extracellular polymeric substances on Microcystis aeruginosa exposed to different sizes of polystyrene microplastics.
36375605	9	32	theme	EPS	1613:1615	arg1	components					1617:1626	different EPS components	1603:1626	different EPS components	1603:1626	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	8	33	theme	polysaccharides	1245:1259	arg1	polysaccharides					1245:1259	polysaccharides	1245:1259	polysaccharides	1245:1259	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	8	33	theme	polysaccharides	1245:1259	arg1	amounts					1234:1240	High amounts	1229:1240	High amounts of polysaccharides	1229:1259	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	5	34	theme	adverse	728:734	arg1	effects					736:742	the adverse effects	724:742	the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress	724:819	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	4	35	theme	different	660:668	arg1	sizes					670:674	different sizes	660:674	different sizes	660:674	The results indicated that the EPS compositions were different when M. aeruginosa was exposed to PS MPs of different sizes.
36375605	9	36	theme	components	1617:1626	arg1	roles					1594:1598	the roles	1590:1598	the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa	1590:1686	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	1	37	theme	external	227:234	arg1	stresses					236:243	external stresses	227:243	external stresses	227:243	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	3	38	theme	PS	415:416	arg1	100 nm					431:436	100 nm	431:436	100 nm	431:436	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	38	theme	PS	415:416	arg1	1 μm					443:446	1 μm	443:446	1 μm	443:446	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	38	theme	PS	415:416	arg1	20 nm					424:428	20 nm	424:428	20 nm	424:428	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	38	theme	PS	415:416	arg1	MPs					419:421	polystyrene (PS) MPs	402:421	polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm)	402:447	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	0	39	theme	substances	33:42	arg1	Roles					0:4	Roles	0:4	Roles of extracellular polymeric substances on Microcystis aeruginosa	0:68	Roles of extracellular polymeric substances on Microcystis aeruginosa exposed to different sizes of polystyrene microplastics.
36375605	3	40	dep	MPs	419:421	arg1	100 nm					431:436	100 nm	431:436	100 nm	431:436	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	40	dep	MPs	419:421	arg1	1 μm					443:446	1 μm	443:446	1 μm	443:446	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	40	dep	MPs	419:421	arg1	20 nm					424:428	20 nm	424:428	20 nm	424:428	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	40	dep	MPs	419:421	arg1	MPs					419:421	polystyrene (PS) MPs	402:421	polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm)	402:447	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	5	41	theme	M.	757:758	arg1	growth					776:781	M. aeruginosa cell growth	757:781	M. aeruginosa cell growth	757:781	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	7	42	theme	EPS	1053:1055	arg1	proteins					1008:1015	aromatic proteins	999:1015	aromatic proteins	999:1015	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	42	theme	EPS	1053:1055	arg1	acids					1028:1032	fulvic acids	1021:1032	fulvic acids	1021:1032	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	42	theme	EPS	1053:1055	arg1	components					1057:1066	representative EPS components	1038:1066	representative EPS components stimulated by 100 nm PS MPs	1038:1094	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	5	43	theme	PS	747:748	arg1	MPs					750:752	PS MPs	747:752	PS MPs	747:752	The presence of EPS is helpful for alleviating the adverse effects of PS MPs on M. aeruginosa cell growth, photosynthesis, and oxidative stress.
36375605	7	44	theme	cells	1161:1165	arg1	self-aggregation					1117:1132	self-aggregation	1117:1132	self-aggregation	1117:1132	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	44	theme	cells	1161:1165	arg1	availability					1171:1182	availability	1171:1182	availability of nutrients for algal growth	1171:1212	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	44	theme	cells	1161:1165	arg1	encapsulation					1138:1150	encapsulation	1138:1150	encapsulation	1138:1150	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	9	45	theme	adverse	1645:1651	arg1	effects					1653:1659	the adverse effects	1641:1659	the adverse effects of PS MPs on M. aeruginosa	1641:1686	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	4	46	theme	sizes	670:674	arg1	MPs					653:655	PS MPs	650:655	PS MPs of different sizes	650:674	The results indicated that the EPS compositions were different when M. aeruginosa was exposed to PS MPs of different sizes.
36375605	3	47	theme	polystyrene	402:412	arg1	100 nm					431:436	100 nm	431:436	100 nm	431:436	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	47	theme	polystyrene	402:412	arg1	1 μm					443:446	1 μm	443:446	1 μm	443:446	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	47	theme	polystyrene	402:412	arg1	20 nm					424:428	20 nm	424:428	20 nm	424:428	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	3	47	theme	polystyrene	402:412	arg1	MPs					419:421	polystyrene (PS) MPs	402:421	polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm)	402:447	In this study, three sizes of polystyrene (PS) MPs (20 nm, 100 nm, and 1 μm) were chosen for evaluating the compositions of EPS, secreted by Microcystis aeruginosa during exposure.
36375605	6	48	theme	insufficient	864:875	arg1	EPS					877:879	insufficient EPS	864:879	insufficient EPS	864:879	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	9	49	theme	different	1603:1611	arg1	components					1617:1626	different EPS components	1603:1626	different EPS components	1603:1626	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	8	50	theme	20 nm PS	1334:1341	arg1	MPs					1343:1345	20 nm PS MPs	1334:1345	20 nm PS MPs	1334:1345	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	1	51	theme	polymeric	141:149	arg1	substances					151:160	Extracellular polymeric substances	127:160	Extracellular polymeric substances (EPS)	127:166	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	1	51	theme	polymeric	141:149	arg1	shields					182:188	important shields	172:188	important shields for microalgae	172:203	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	1	51	theme	polymeric	141:149	arg1	EPS					163:165	EPS	163:165	EPS	163:165	Extracellular polymeric substances (EPS) are important shields for microalgae when confronting with external stresses.
36375605	7	52	theme	100 nm PS	1082:1090	arg1	MPs					1092:1094	100 nm PS MPs	1082:1094	100 nm PS MPs	1082:1094	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	53	dep	self-aggregation	1117:1132	arg1	the					1113:1115	the	1113:1115	the	1113:1115	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	2	54	theme	EPS	279:281	arg1	roles					270:274	the underlying roles	255:274	the underlying roles of EPS in the interactions between microplastics (MPs) and microalgae	255:344	However, the underlying roles of EPS in the interactions between microplastics (MPs) and microalgae remain poorly understood.
36375605	9	55	theme	MPs	1553:1555	arg1	stimulation					1535:1545	the stimulation	1531:1545	the stimulation of PS MPs of different sizes	1531:1574	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	7	56	from	components	1057:1066	arg1	contrast					989:996	contrast	989:996	contrast	989:996	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	9	57	from	differences	1506:1516	arg1	EPS					1521:1523	EPS	1521:1523	EPS	1521:1523	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	9	58	theme	MPs	1667:1669	arg1	effects					1653:1659	the adverse effects	1641:1659	the adverse effects of PS MPs on M. aeruginosa	1641:1686	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	8	59	theme	MPs	1410:1412	arg1	homo-aggregation					1381:1396	the homo-aggregation	1377:1396	the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa	1377:1465	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	7	60	theme	algal	1201:1205	arg1	growth					1207:1212	algal growth	1201:1212	algal growth	1201:1212	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	9	61	theme	sizes	1570:1574	arg1	MPs					1553:1555	PS MPs	1550:1555	PS MPs of different sizes	1550:1574	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	9	62	from	effects	1653:1659	arg1	aeruginosa					1677:1686	M. aeruginosa	1674:1686	M. aeruginosa	1674:1686	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	7	63	theme	algal	1155:1159	arg1	cells					1161:1165	algal cells	1155:1165	algal cells	1155:1165	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	8	64	theme	20 nm PS	1401:1408	arg1	MPs					1410:1412	20 nm PS MPs	1401:1412	20 nm PS MPs	1401:1412	High amounts of polysaccharides were secreted by M. aeruginosa along with humic acids during exposure to 20 nm PS MPs, both of which are crucial in the homo-aggregation of 20 nm PS MPs toward minimize its adverse effects on M. aeruginosa.
36375605	9	65	theme	PS	1550:1551	arg1	MPs					1553:1555	PS MPs	1550:1555	PS MPs of different sizes	1550:1574	Together, these findings revealed the differences in EPS under the stimulation of PS MPs of different sizes and clarified the roles of different EPS components in resisting the adverse effects of PS MPs on M. aeruginosa.
36375605	6	66	theme	cell	975:978	arg1	wall					980:983	the cell wall	971:983	the cell wall	971:983	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	4	67	theme	PS	650:651	arg1	MPs					653:655	PS MPs	650:655	PS MPs of different sizes	650:674	The results indicated that the EPS compositions were different when M. aeruginosa was exposed to PS MPs of different sizes.
36375605	4	68	theme	EPS	584:586	arg1	different					606:614	different	606:614	different	606:614	The results indicated that the EPS compositions were different when M. aeruginosa was exposed to PS MPs of different sizes.
36375605	4	68	theme	EPS	584:586	arg1	compositions					588:599	the EPS compositions	580:599	the EPS compositions	580:599	The results indicated that the EPS compositions were different when M. aeruginosa was exposed to PS MPs of different sizes.
36375605	6	69	theme	MPs	925:927	arg1	adsorption					894:903	direct adsorption	887:903	direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall	887:983	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	6	70	theme	effect	856:861	arg1	exception					831:839	the exception	827:839	the exception of the shading effect	827:861	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
36375605	2	71	theme	underlying	259:268	arg1	roles					270:274	the underlying roles	255:274	the underlying roles of EPS in the interactions between microplastics (MPs) and microalgae	255:344	However, the underlying roles of EPS in the interactions between microplastics (MPs) and microalgae remain poorly understood.
36375605	7	72	theme	nutrients	1187:1195	arg1	self-aggregation					1117:1132	self-aggregation	1117:1132	self-aggregation	1117:1132	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	72	theme	nutrients	1187:1195	arg1	availability					1171:1182	availability	1171:1182	availability of nutrients for algal growth	1171:1212	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	7	72	theme	nutrients	1187:1195	arg1	encapsulation					1138:1150	encapsulation	1138:1150	encapsulation	1138:1150	In contrast, aromatic proteins and fulvic acids are representative EPS components stimulated by 100 nm PS MPs, contributing to the self-aggregation and encapsulation of algal cells and availability of nutrients for algal growth, respectively.
36375605	6	73	theme	algal	936:940	arg1	surface					942:948	the algal surface	932:948	the algal surface	932:948	With the exception of the shading effect, insufficient EPS cause direct adsorption of unstable 1 μm PS MPs to the algal surface, which could destroy the cell wall.
35740409	10	0	theme	mechanical	1324:1333	arg1	properties					1335:1344	its structural and mechanical properties	1305:1344	its structural and mechanical properties	1305:1344	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	1	1	theme	much	187:190	arg1	attention					192:200	much attention	187:200	much attention	187:200	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	10	2	theme	promising	1355:1363	arg1	potential					1365:1373	a promising potential	1353:1373	a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages	1353:1493	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	10	3	theme	structural	1309:1318	arg1	properties					1335:1344	its structural and mechanical properties	1305:1344	its structural and mechanical properties	1305:1344	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	0	4	theme	Tissue	96:101	arg1	Engineering					103:113	Tissue Engineering	96:113	Tissue Engineering	96:113	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering.
35740409	4	5	theme	diphenylalanine	558:572	arg1	hydrogelator					591:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	(PS), it was integrated with the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator.
35740409	9	6	theme	MC3T3-E1	1142:1149	arg1	viability					1166:1174	viability	1166:1174	viability	1166:1174	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	9	6	theme	MC3T3-E1	1142:1149	arg1	deposition					1188:1197	calcium deposition	1180:1197	calcium deposition	1180:1197	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	9	6	theme	MC3T3-E1	1142:1149	arg1	preosteoblasts					1151:1164	MC3T3-E1 preosteoblasts viability and calcium deposition	1142:1197	MC3T3-E1 preosteoblasts viability and calcium deposition	1142:1197	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	4	7	theme	fluorenylmethoxycarbonyl	533:556	arg1	hydrogelator					591:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	(PS), it was integrated with the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator.
35740409	5	8	theme	different	702:710	arg1	concentrations					712:725	three different concentrations	696:725	three different concentrations	696:725	Transparent, stable hydrogels were formed when mixing the two components at a 1:1 ratio in three different concentrations.
35740409	5	9	dep	Transparent	605:615	arg1	stable					618:623	stable	618:623	stable	618:623	Transparent, stable hydrogels were formed when mixing the two components at a 1:1 ratio in three different concentrations.
35740409	8	10	from	change	1071:1076	arg1	concentration					1094:1106	the hydrogel concentration	1081:1106	the hydrogel concentration	1081:1106	The composite hydrogels allowed the sustained release of curcumin which was controlled by the change in the hydrogel concentration.
35740409	10	11	theme	tunable	1396:1402	arg1	scaffolds					1404:1412	novel tunable scaffolds	1390:1412	novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages	1390:1493	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	10	12	theme	tissue	1418:1423	arg1	engineering					1425:1435	tissue engineering	1418:1435	tissue engineering	1418:1435	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	3	13	theme	Porphyridium	483:494	arg1	microalga					472:480	the red marine microalga	457:480	the red marine microalga	457:480	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	3	13	theme	Porphyridium	483:494	arg1	sp					496:497	Porphyridium sp	483:497	Porphyridium sp	483:497	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	3	14	theme	mechanical	392:401	arg1	properties					403:412	the mechanical properties	388:412	the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp	388:497	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	3	15	theme	red	461:463	arg1	microalga					472:480	the red marine microalga	457:480	the red marine microalga	457:480	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	3	15	theme	red	461:463	arg1	sp					496:497	Porphyridium sp	483:497	Porphyridium sp	483:497	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	9	16	theme	calcium	1180:1186	arg1	deposition					1188:1197	calcium deposition	1180:1197	calcium deposition	1180:1197	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	9	16	theme	calcium	1180:1186	arg1	preosteoblasts					1151:1164	MC3T3-E1 preosteoblasts viability and calcium deposition	1142:1197	MC3T3-E1 preosteoblasts viability and calcium deposition	1142:1197	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	1	17	theme	biomedical	206:215	arg1	applications					217:228	biomedical applications	206:228	biomedical applications due to their anti-inflammatory and antioxidant properties	206:286	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	0	18	theme	Red	12:14	arg1	Hydrogels					69:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels	0:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering	0:113	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering.
35740409	9	19	dep	preosteoblasts	1151:1164	arg1	viability					1166:1174	viability	1166:1174	viability	1166:1174	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	9	19	dep	preosteoblasts	1151:1164	arg1	deposition					1188:1197	calcium deposition	1180:1197	calcium deposition	1180:1197	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	9	19	dep	preosteoblasts	1151:1164	arg1	preosteoblasts					1151:1164	MC3T3-E1 preosteoblasts viability and calcium deposition	1142:1197	MC3T3-E1 preosteoblasts viability and calcium deposition	1142:1197	Finally, the hydrogels supported MC3T3-E1 preosteoblasts viability and calcium deposition.
35740409	0	20	theme	Thixotropic	0:10	arg1	Hydrogels					69:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels	0:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering	0:113	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering.
35740409	4	21	dep	integrated	513:522	arg1	PS					501:502	PS	501:502	PS	501:502	(PS), it was integrated with the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator.
35740409	5	22	theme	1:1	683:685	arg1	ratio					687:691	a 1:1 ratio	681:691	a 1:1 ratio in three different concentrations	681:725	Transparent, stable hydrogels were formed when mixing the two components at a 1:1 ratio in three different concentrations.
35740409	5	23	from	ratio	687:691	arg1	concentrations					712:725	three different concentrations	696:725	three different concentrations	696:725	Transparent, stable hydrogels were formed when mixing the two components at a 1:1 ratio in three different concentrations.
35740409	1	24	theme	Sulfated	116:123	arg1	polysaccharides					125:139	Sulfated polysaccharides	116:139	Sulfated polysaccharides of red marine microalgae	116:164	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	7	25	theme	tunable	893:899	arg1	properties					912:921	tunable mechanical properties	893:921	tunable mechanical properties	893:921	Furthermore, the hydrogels were injectable, and tunable mechanical properties were obtained by changing the hydrogel concentration.
35740409	1	26	theme	due	230:232	arg1	applications					217:228	biomedical applications	206:228	biomedical applications due to their anti-inflammatory and antioxidant properties	206:286	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	0	27	theme	Sulfated	27:34	arg1	Hydrogels					69:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels	0:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering	0:113	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering.
35740409	8	28	theme	hydrogel	1085:1092	arg1	concentration					1094:1106	the hydrogel concentration	1081:1106	the hydrogel concentration	1081:1106	The composite hydrogels allowed the sustained release of curcumin which was controlled by the change in the hydrogel concentration.
35740409	2	29	theme	tissue	349:354	arg1	engineering					356:366	tissue engineering	349:366	tissue engineering	349:366	However, their low mechanical properties limit their use in tissue engineering.
35740409	6	30	theme	Electron	728:735	arg1	microscopy					737:746	Electron microscopy	728:746	Electron microscopy	728:746	Electron microscopy showed that all hydrogels exhibited a nanofibrous structure, mimicking the extracellular matrix.
35740409	0	31	theme	Microalgae	16:25	arg1	Hydrogels					69:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels	0:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering	0:113	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering.
35740409	10	32	theme	sulfated	1224:1231	arg1	polysaccharide					1233:1246	the sulfated polysaccharide	1220:1246	the sulfated polysaccharide	1220:1246	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	0	33	theme	Composite	59:67	arg1	Hydrogels					69:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels	0:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering	0:113	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering.
35740409	10	34	theme	novel	1390:1394	arg1	scaffolds					1404:1412	novel tunable scaffolds	1390:1412	novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages	1390:1493	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	8	35	theme	composite	981:989	arg1	hydrogels					991:999	The composite hydrogels	977:999	The composite hydrogels	977:999	The composite hydrogels allowed the sustained release of curcumin which was controlled by the change in the hydrogel concentration.
35740409	1	36	theme	anti-inflammatory	243:259	arg1	properties					277:286	their anti-inflammatory and antioxidant properties	237:286	their anti-inflammatory and antioxidant properties	237:286	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	0	37	theme	Polysaccharide-Peptide	36:57	arg1	Hydrogels					69:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels	0:77	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering	0:113	Thixotropic Red Microalgae Sulfated Polysaccharide-Peptide Composite Hydrogels as Scaffolds for Tissue Engineering.
35740409	4	38	theme	FmocFF	575:580	arg1	hydrogelator					591:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	(PS), it was integrated with the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator.
35740409	3	39	theme	marine	465:470	arg1	microalga					472:480	the red marine microalga	457:480	the red marine microalga	457:480	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	3	39	theme	marine	465:470	arg1	sp					496:497	Porphyridium sp	483:497	Porphyridium sp	483:497	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	10	40	theme	cell	1452:1455	arg1	differentiation					1457:1471	cell differentiation	1452:1471	cell differentiation into various lineages	1452:1493	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	6	41	theme	extracellular	823:835	arg1	matrix					837:842	the extracellular matrix	819:842	the extracellular matrix	819:842	Electron microscopy showed that all hydrogels exhibited a nanofibrous structure, mimicking the extracellular matrix.
35740409	10	42	with	synergy	1204:1210	arg1	properties					1335:1344	its structural and mechanical properties	1305:1344	its structural and mechanical properties	1305:1344	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	10	42	with	synergy	1204:1210	arg1	peptide					1291:1297	FmocFF peptide	1284:1297	FmocFF peptide	1284:1297	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	10	42	with	synergy	1204:1210	arg1	bioactivities					1265:1277	its unique bioactivities	1254:1277	its unique bioactivities	1254:1277	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	10	43	theme	unique	1258:1263	arg1	bioactivities					1265:1277	its unique bioactivities	1254:1277	its unique bioactivities	1254:1277	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	1	44	theme	red	144:146	arg1	microalgae					155:164	red marine microalgae	144:164	red marine microalgae	144:164	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	4	45	theme	peptide	583:589	arg1	hydrogelator					591:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator	529:602	(PS), it was integrated with the fluorenylmethoxycarbonyl diphenylalanine (FmocFF) peptide hydrogelator.
35740409	10	46	contain	bears	1347:1351	arg2	potential					1365:1373	a promising potential	1353:1373	a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages	1353:1493	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	10	46	contain	bears	1347:1351	arg1	synergy					1204:1210	The synergy	1200:1210	The synergy	1200:1210	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	5	47	theme	Transparent	605:615	arg1	hydrogels					625:633	Transparent, stable hydrogels	605:633	Transparent, stable hydrogels	605:633	Transparent, stable hydrogels were formed when mixing the two components at a 1:1 ratio in three different concentrations.
35740409	1	48	theme	marine	148:153	arg1	microalgae					155:164	red marine microalgae	144:164	red marine microalgae	144:164	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	2	49	theme	mechanical	308:317	arg1	properties					319:328	their low mechanical properties	298:328	their low mechanical properties	298:328	However, their low mechanical properties limit their use in tissue engineering.
35740409	8	50	theme	sustained	1013:1021	arg1	release					1023:1029	the sustained release	1009:1029	the sustained release of curcumin which was controlled by the change in the hydrogel concentration	1009:1106	The composite hydrogels allowed the sustained release of curcumin which was controlled by the change in the hydrogel concentration.
35740409	1	51	theme	microalgae	155:164	arg1	polysaccharides					125:139	Sulfated polysaccharides	116:139	Sulfated polysaccharides of red marine microalgae	116:164	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	1	52	theme	antioxidant	265:275	arg1	properties					277:286	their anti-inflammatory and antioxidant properties	237:286	their anti-inflammatory and antioxidant properties	237:286	Sulfated polysaccharides of red marine microalgae have recently gained much attention for biomedical applications due to their anti-inflammatory and antioxidant properties.
35740409	2	53	theme	low	304:306	arg1	properties					319:328	their low mechanical properties	298:328	their low mechanical properties	298:328	However, their low mechanical properties limit their use in tissue engineering.
35740409	8	54	theme	curcumin	1034:1041	arg1	release					1023:1029	the sustained release	1009:1029	the sustained release of curcumin which was controlled by the change in the hydrogel concentration	1009:1106	The composite hydrogels allowed the sustained release of curcumin which was controlled by the change in the hydrogel concentration.
35740409	7	55	theme	mechanical	901:910	arg1	properties					912:921	tunable mechanical properties	893:921	tunable mechanical properties	893:921	Furthermore, the hydrogels were injectable, and tunable mechanical properties were obtained by changing the hydrogel concentration.
35740409	2	56	from	use	342:344	arg1	engineering					356:366	tissue engineering	349:366	tissue engineering	349:366	However, their low mechanical properties limit their use in tissue engineering.
35740409	10	57	theme	FmocFF	1284:1289	arg1	peptide					1291:1297	FmocFF peptide	1284:1297	FmocFF peptide	1284:1297	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	10	58	theme	various	1478:1484	arg1	lineages					1486:1493	various lineages	1478:1493	various lineages	1478:1493	The synergy between the sulfated polysaccharide, with its unique bioactivities, and FmocFF peptide, with its structural and mechanical properties, bears a promising potential for developing novel tunable scaffolds for tissue engineering that may allow cell differentiation into various lineages.
35740409	6	59	theme	nanofibrous	786:796	arg1	structure					798:806	a nanofibrous structure	784:806	a nanofibrous structure	784:806	Electron microscopy showed that all hydrogels exhibited a nanofibrous structure, mimicking the extracellular matrix.
35740409	3	60	theme	sulfated	421:428	arg1	polysaccharide					430:443	the sulfated polysaccharide	417:443	the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp	417:497	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	3	61	theme	polysaccharide	430:443	arg1	properties					403:412	the mechanical properties	388:412	the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp	388:497	Herein, to enhance the mechanical properties of the sulfated polysaccharide produced by the red marine microalga, Porphyridium sp.
35740409	7	62	theme	hydrogel	953:960	arg1	concentration					962:974	the hydrogel concentration	949:974	the hydrogel concentration	949:974	Furthermore, the hydrogels were injectable, and tunable mechanical properties were obtained by changing the hydrogel concentration.
36034663	1	0	theme	quality	243:249	arg1	system					262:267	appropriate quality evaluation system	231:267	appropriate quality evaluation system of TCM	231:274	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	2	1	theme	hydrolytic	504:513	arg1	product					515:521	the hydrolytic product	500:521	the hydrolytic product of P. sibiricum polysaccharides (PSPs)	500:560	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	6	2	theme	excellent	1203:1211	arg1	precision					1213:1221	excellent precision	1203:1221	excellent precision	1203:1221	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	7	3	theme	fluorescence	1516:1527	arg1	determination					1529:1541	fluorescence determination	1516:1541	fluorescence determination of different monosaccharides	1516:1570	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	1	4	theme	evaluation	251:260	arg1	system					262:267	appropriate quality evaluation system	231:267	appropriate quality evaluation system of TCM	231:274	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	3	5	from	reaction	656:663	arg1	water					668:672	water	668:672	water	668:672	The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars.
36034663	0	6	from	Synthesis	0:8	arg1	evaluation					74:83	quality evaluation	66:83	quality evaluation	66:83	Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red.
36034663	5	7	theme	GlcA	1124:1127	arg1	16.67:52.94:10.58:19.81					1141:1163	16.67:52.94:10.58:19.81	1141:1163	16.67:52.94:10.58:19.81	1141:1163	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	7	theme	GlcA	1124:1127	arg1	ratio					1105:1109	the molar ratio	1095:1109	the molar ratio of Glc, Gal, GlcA and GalA	1095:1136	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	4	8	theme	Good	750:753	arg1	separation					755:764	Good separation	750:764	Good separation	750:764	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	3	9	theme	reducing	733:740	arg1	sugars					742:747	reducing sugars	733:747	reducing sugars	733:747	The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars.
36034663	4	10	theme	flow	874:877	arg1	rate					879:882	a flow rate	872:882	a flow rate of 0.5 ml/min within 12 min	872:910	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	7	11	theme	push-pull	1388:1396	arg1	mode					1399:1402	"push-pull" mode	1387:1402	"push-pull" mode	1387:1402	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	7	12	theme	"	1450:1450	arg1	mode					1452:1455	"pull-pull" mode	1440:1455	"pull-pull" mode	1440:1455	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	2	13	theme	polysaccharides	539:553	arg1	product					515:521	the hydrolytic product	500:521	the hydrolytic product of P. sibiricum polysaccharides (PSPs)	500:560	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	0	14	from	application	51:61	arg1	evaluation					74:83	quality evaluation	66:83	quality evaluation	66:83	Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red.
36034663	1	15	dep	premise	120:126	arg1	The					116:118	The	116:118	The	116:118	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	6	16	contain	possessing	1192:1201	arg2	precision					1213:1221	excellent precision	1203:1221	excellent precision	1203:1221	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	6	16	contain	possessing	1192:1201	arg1	method					1184:1189	The verified HPLC method	1166:1189	The verified HPLC method	1166:1189	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	6	16	contain	possessing	1192:1201	arg2	accuracy					1232:1239	good accuracy	1227:1239	good accuracy	1227:1239	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	2	17	theme	sibiricum	396:404	arg1	control					373:379	the quality control	361:379	the quality control of Polygonatum sibiricum	361:404	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	2	18	theme	sibiricum	529:537	arg1	polysaccharides					539:553	P. sibiricum polysaccharides	526:553	P. sibiricum polysaccharides (PSPs)	526:560	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	2	18	theme	sibiricum	529:537	arg1	PSPs					556:559	PSPs	556:559	PSPs	556:559	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	5	19	theme	galacturonic	1064:1075	arg1	GalA					1083:1086	GalA	1083:1086	GalA	1083:1086	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	19	theme	galacturonic	1064:1075	arg1	acid					1077:1080	galacturonic acid	1064:1080	galacturonic acid (GalA)]	1064:1088	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	19	theme	galacturonic	1064:1075	arg1	acids					1030:1034	two hexuronic acids	1016:1034	two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)]	1016:1088	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	6	20	theme	HPLC	1179:1182	arg1	method					1184:1189	The verified HPLC method	1166:1189	The verified HPLC method	1166:1189	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	6	21	theme	quantitative	1286:1297	arg1	determination					1299:1311	rapid qualitative and quantitative determination	1264:1311	rapid qualitative and quantitative determination for PSP	1264:1319	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	6	22	theme	verified	1170:1177	arg1	method					1184:1189	The verified HPLC method	1166:1189	The verified HPLC method	1166:1189	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	1	23	dep	safety	152:157	arg1	the					148:150	the	148:150	the	148:150	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	1	24	theme	traditional	180:190	arg1	TCM					210:212	TCM	210:212	TCM	210:212	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	1	24	theme	traditional	180:190	arg1	medicine					200:207	traditional Chinese medicine	180:207	traditional Chinese medicine (TCM)	180:213	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	1	25	theme	TCM	272:274	arg1	system					262:267	appropriate quality evaluation system	231:267	appropriate quality evaluation system of TCM	231:274	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	4	26	theme	gradient	852:859	arg1	elution					861:867	a gradient elution	850:867	a gradient elution at a flow rate of 0.5 ml/min within 12 min	850:910	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	5	27	theme	PSP	948:950	arg1	compositions					932:943	The monosaccharide compositions	913:943	The monosaccharide compositions of PSP	913:950	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	6	28	theme	rapid	1264:1268	arg1	determination					1299:1311	rapid qualitative and quantitative determination	1264:1311	rapid qualitative and quantitative determination for PSP	1264:1319	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	1	29	theme	Chinese	192:198	arg1	TCM					210:212	TCM	210:212	TCM	210:212	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	1	29	theme	Chinese	192:198	arg1	medicine					200:207	traditional Chinese medicine	180:207	traditional Chinese medicine (TCM)	180:213	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	0	30	theme	chemsensor	32:41	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of naphthalimide-type chemsensor	0:41	Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red.
36034663	0	30	theme	chemsensor	32:41	arg1	application					51:61	its application	47:61	its application in quality evaluation for polygonatum sibiricum Red	47:113	Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red.
36034663	7	31	theme	fluorescence	1354:1365	arg1	enhancement					1367:1377	fluorescence enhancement	1354:1377	fluorescence enhancement	1354:1377	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	6	32	theme	qualitative	1270:1280	arg1	determination					1299:1311	rapid qualitative and quantitative determination	1264:1311	rapid qualitative and quantitative determination for PSP	1264:1319	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	1	33	theme	medicine	200:207	arg1	effectiveness					163:175	effectiveness	163:175	effectiveness	163:175	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	1	33	theme	medicine	200:207	arg1	safety					152:157	safety	152:157	safety	152:157	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
36034663	0	34	theme	naphthalimide-type	13:30	arg1	chemsensor					32:41	naphthalimide-type chemsensor	13:41	naphthalimide-type chemsensor	13:41	Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red.
36034663	5	35	theme	Glc	1114:1116	arg1	16.67:52.94:10.58:19.81					1141:1163	16.67:52.94:10.58:19.81	1141:1163	16.67:52.94:10.58:19.81	1141:1163	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	35	theme	Glc	1114:1116	arg1	ratio					1105:1109	the molar ratio	1095:1109	the molar ratio of Glc, Gal, GlcA and GalA	1095:1136	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	4	36	theme	detection	784:792	arg1	wavelength					794:803	a detection wavelength	782:803	a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min	782:910	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	2	37	from	product	515:521	arg1	monosaccharides					479:493	diverse monosaccharides	471:493	diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs)	471:560	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	2	38	theme	synthesized	418:428	arg1	HAN					461:463	HAN	461:463	HAN	461:463	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	2	38	theme	synthesized	418:428	arg1	4-hydrazino-1,8-naphthalimide					430:458	synthesized 4-hydrazino-1,8-naphthalimide	418:458	synthesized 4-hydrazino-1,8-naphthalimide (HAN)	418:464	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	4	39	theme	ZORBAX	824:829	arg1	column					837:842	an ZORBAX SB-C8 column	821:842	an ZORBAX SB-C8 column	821:842	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	5	40	dep	acids	1030:1034	arg1	GalA					1083:1086	GalA	1083:1086	GalA	1083:1086	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	40	dep	acids	1030:1034	arg1	acid					1048:1051	[glucuronic acid	1036:1051	[glucuronic acid (GlcA)	1036:1058	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	40	dep	acids	1030:1034	arg1	acid					1077:1080	galacturonic acid	1064:1080	galacturonic acid (GalA)]	1064:1088	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	40	dep	acids	1030:1034	arg1	acids					1030:1034	two hexuronic acids	1016:1034	two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)]	1016:1088	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	40	dep	acids	1030:1034	arg1	GlcA					1054:1057	GlcA	1054:1057	GlcA	1054:1057	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	7	41	theme	monosaccharides	1556:1570	arg1	determination					1529:1541	fluorescence determination	1516:1541	fluorescence determination of different monosaccharides	1516:1570	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	5	42	theme	[glucuronic	1036:1046	arg1	acid					1048:1051	[glucuronic acid	1036:1051	[glucuronic acid (GlcA)	1036:1058	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	42	theme	[glucuronic	1036:1046	arg1	acids					1030:1034	two hexuronic acids	1016:1034	two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)]	1016:1088	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	42	theme	[glucuronic	1036:1046	arg1	GlcA					1054:1057	GlcA	1054:1057	GlcA	1054:1057	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	4	43	theme	0.5 ml/min	887:896	arg1	rate					879:882	a flow rate	872:882	a flow rate of 0.5 ml/min within 12 min	872:910	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	7	44	theme	"	1397:1397	arg1	mode					1399:1402	"push-pull" mode	1387:1402	"push-pull" mode	1387:1402	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	2	45	theme	diverse	471:477	arg1	monosaccharides					479:493	diverse monosaccharides	471:493	diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs)	471:560	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	7	46	theme	different	1546:1554	arg1	monosaccharides					1556:1570	different monosaccharides	1546:1570	different monosaccharides	1546:1570	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	4	47	theme	SB-C8	831:835	arg1	column					837:842	an ZORBAX SB-C8 column	821:842	an ZORBAX SB-C8 column	821:842	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	2	48	theme	HPLC	335:338	arg1	method					340:345	a pre-column derivatization HPLC method	307:345	a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation	307:589	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	5	49	theme	molar	1099:1103	arg1	16.67:52.94:10.58:19.81					1141:1163	16.67:52.94:10.58:19.81	1141:1163	16.67:52.94:10.58:19.81	1141:1163	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	49	theme	molar	1099:1103	arg1	ratio					1105:1109	the molar ratio	1095:1109	the molar ratio of Glc, Gal, GlcA and GalA	1095:1136	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	6	50	theme	good	1227:1230	arg1	accuracy					1232:1239	good accuracy	1227:1239	good accuracy	1227:1239	The verified HPLC method, possessing excellent precision and good accuracy, successfully achieved rapid qualitative and quantitative determination for PSP.
36034663	7	51	theme	pull-pull	1441:1449	arg1	mode					1452:1455	"pull-pull" mode	1440:1455	"pull-pull" mode	1440:1455	Additionally, the HAN displayed fluorescence enhancement through "push-pull" mode, and fluorescence decreased through "pull-pull" mode after binding to monosaccharides, which is a potential for fluorescence determination of different monosaccharides.
36034663	3	52	theme	CuI-catalyzed	627:639	arg1	reaction					656:663	a CuI-catalyzed cross-coupling reaction	625:663	a CuI-catalyzed cross-coupling reaction in water	625:672	The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars.
36034663	2	53	theme	derivatization	320:333	arg1	method					340:345	a pre-column derivatization HPLC method	307:345	a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation	307:589	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	5	54	theme	GalA	1133:1136	arg1	16.67:52.94:10.58:19.81					1141:1163	16.67:52.94:10.58:19.81	1141:1163	16.67:52.94:10.58:19.81	1141:1163	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	54	theme	GalA	1133:1136	arg1	ratio					1105:1109	the molar ratio	1095:1109	the molar ratio of Glc, Gal, GlcA and GalA	1095:1136	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	2	55	theme	HPLC	575:578	arg1	separation					580:589	HPLC separation	575:589	HPLC separation	575:589	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	5	56	theme	hexuronic	1020:1028	arg1	acid					1048:1051	[glucuronic acid	1036:1051	[glucuronic acid (GlcA)	1036:1058	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	56	theme	hexuronic	1020:1028	arg1	acids					1030:1034	two hexuronic acids	1016:1034	two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)]	1016:1088	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	56	theme	hexuronic	1020:1028	arg1	acid					1077:1080	galacturonic acid	1064:1080	galacturonic acid (GalA)]	1064:1088	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	3	57	theme	cross-coupling	641:654	arg1	reaction					656:663	a CuI-catalyzed cross-coupling reaction	625:663	a CuI-catalyzed cross-coupling reaction in water	625:672	The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars.
36034663	2	58	theme	pre-column	309:318	arg1	method					340:345	a pre-column derivatization HPLC method	307:345	a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation	307:589	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	5	59	theme	monosaccharide	917:930	arg1	compositions					932:943	The monosaccharide compositions	913:943	The monosaccharide compositions of PSP	913:950	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	2	60	theme	P.	526:527	arg1	polysaccharides					539:553	P. sibiricum polysaccharides	526:553	P. sibiricum polysaccharides (PSPs)	526:560	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	2	60	theme	P.	526:527	arg1	PSPs					556:559	PSPs	556:559	PSPs	556:559	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	5	61	theme	[glucose	979:986	arg1	hexoses					971:977	two hexoses [glucose	967:986	two hexoses [glucose (Glc)	967:992	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	5	61	theme	[glucose	979:986	arg1	Glc					989:991	Glc	989:991	Glc	989:991	The monosaccharide compositions of PSP mainly include two hexoses [glucose (Glc), galactose (Gal)] and two hexuronic acids [glucuronic acid (GlcA) and galacturonic acid (GalA)], and the molar ratio of Glc, Gal, GlcA and GalA is 16.67:52.94:10.58:19.81.
36034663	3	62	theme	novel	698:702	arg1	chemosensor					704:714	a novel chemosensor	696:714	a novel chemosensor that reacts with reducing sugars	696:747	The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars.
36034663	3	62	theme	novel	698:702	arg1	HAN					596:598	The HAN	592:598	The HAN	592:598	The HAN was synthesized based on a CuI-catalyzed cross-coupling reaction in water, and then employed as a novel chemosensor that reacts with reducing sugars.
36034663	2	63	theme	quality	365:371	arg1	control					373:379	the quality control	361:379	the quality control of Polygonatum sibiricum	361:404	This study aimed to establish a pre-column derivatization HPLC method for achieving the quality control of Polygonatum sibiricum by reacting synthesized 4-hydrazino-1,8-naphthalimide (HAN) with diverse monosaccharides from the hydrolytic product of P. sibiricum polysaccharides (PSPs), followed by HPLC separation.
36034663	0	64	theme	quality	66:72	arg1	evaluation					74:83	quality evaluation	66:83	quality evaluation	66:83	Synthesis of naphthalimide-type chemsensor and its application in quality evaluation for polygonatum sibiricum Red.
36034663	4	65	from	rate	879:882	arg1	elution					861:867	a gradient elution	850:867	a gradient elution at a flow rate of 0.5 ml/min within 12 min	850:910	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	4	66	theme	448 nm	808:813	arg1	wavelength					794:803	a detection wavelength	782:803	a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min	782:910	Good separation was achieved at a detection wavelength of 448 nm using an ZORBAX SB-C8 column under a gradient elution at a flow rate of 0.5 ml/min within 12 min.
36034663	1	67	theme	appropriate	231:241	arg1	system					262:267	appropriate quality evaluation system	231:267	appropriate quality evaluation system of TCM	231:274	The premise and key of ensuring the safety and effectiveness of traditional Chinese medicine (TCM) is to construct appropriate quality evaluation system of TCM.
35699654	8	0	theme	crystals	1321:1328	arg1	self-organization					1269:1285	the self-organization	1265:1285	the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp	1265:1344	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	17	1	theme	LC	2753:2754	arg1	composites					2774:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	16	2	theme	liquid	2380:2385	arg1	crystals					2387:2394	colloidal liquid crystals	2370:2394	colloidal liquid crystals	2370:2394	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	16	2	theme	liquid	2380:2385	arg1	dispersions					2425:2435	colloidal self-assembled dispersions	2400:2435	colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects	2400:2496	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	11	3	theme	nanostructured	1789:1802	arg1	particles					1804:1812	CaCO3- and HAp-based nanostructured particles	1768:1812	CaCO3- and HAp-based nanostructured particles with rod and disk shapes	1768:1837	Moreover, we recently developed CaCO3- and HAp-based nanostructured particles with rod and disk shapes.
35699654	5	4	theme	glass-fiber-	718:729	arg1	plastics					758:765	glass-fiber- or carbon-fiber-reinforced plastics	718:765	glass-fiber- or carbon-fiber-reinforced plastics	718:765	Biominerals do not show significantly higher performances than synthetic composites such as glass-fiber- or carbon-fiber-reinforced plastics.
35699654	8	5	theme	CaCO3	1333:1337	arg1	polymers					1298:1305	organic polymers	1290:1305	organic polymers	1290:1305	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	8	5	theme	CaCO3	1333:1337	arg1	crystals					1321:1328	inorganic crystals	1311:1328	inorganic crystals	1311:1328	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	2	6	from	composites	373:382	arg1	nature					283:288	nature	283:288	nature	283:288	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	17	7	theme	composites	2774:2783	arg1	design					2691:2696	material design	2682:2696	material design	2682:2696	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	17	7	theme	composites	2774:2783	arg1	aspects					2715:2721	hierarchical aspects	2702:2721	hierarchical aspects	2702:2721	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	5	8	theme	carbon-fiber-reinforced	734:756	arg1	plastics					758:765	glass-fiber- or carbon-fiber-reinforced plastics	718:765	glass-fiber- or carbon-fiber-reinforced plastics	718:765	Biominerals do not show significantly higher performances than synthetic composites such as glass-fiber- or carbon-fiber-reinforced plastics.
35699654	10	9	theme	called	1544:1549	arg1	additives					1559:1567	called polymer additives	1544:1567	called polymer additives	1544:1567	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	21	10	theme	biocompatible	3430:3442	arg1	components					3444:3453	biocompatible components	3430:3453	biocompatible components	3430:3453	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	16	11	theme	organic	2452:2458	arg1	nano-					2474:2478	anisotropic organic and inorganic nano-	2440:2478	anisotropic organic and inorganic nano-	2440:2478	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	8	12	theme	HAp	1342:1344	arg1	polymers					1298:1305	organic polymers	1290:1305	organic polymers	1290:1305	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	8	12	theme	HAp	1342:1344	arg1	crystals					1321:1328	inorganic crystals	1311:1328	inorganic crystals	1311:1328	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	8	13	theme	functional	1129:1138	arg1	materials.Inspired					1140:1157	functional materials.Inspired	1129:1157	functional materials.Inspired by these biological hard tissues	1129:1190	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	16	14	theme	self-assembled	2410:2423	arg1	crystals					2387:2394	colloidal liquid crystals	2370:2394	colloidal liquid crystals	2370:2394	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	16	14	theme	self-assembled	2410:2423	arg1	dispersions					2425:2435	colloidal self-assembled dispersions	2400:2435	colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects	2400:2496	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	21	15	theme	mild	3353:3356	arg1	conditions					3370:3379	mild and aqueous conditions	3353:3379	mild and aqueous conditions	3353:3379	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	5	16	theme	synthetic	689:697	arg1	composites					699:708	synthetic composites	689:708	synthetic composites such as glass-fiber- or carbon-fiber-reinforced plastics	689:765	Biominerals do not show significantly higher performances than synthetic composites such as glass-fiber- or carbon-fiber-reinforced plastics.
35699654	5	16	theme	synthetic	689:697	arg1	plastics					758:765	glass-fiber- or carbon-fiber-reinforced plastics	718:765	glass-fiber- or carbon-fiber-reinforced plastics	718:765	Biominerals do not show significantly higher performances than synthetic composites such as glass-fiber- or carbon-fiber-reinforced plastics.
35699654	19	17	theme	transmission	3062:3073	arg1	microscopy					3084:3093	high-resolution transmission electron microscopy	3046:3093	high-resolution transmission electron microscopy	3046:3093	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	17	18	dep	states.In	2640:2648	arg1	focused					2667:2673	focused	2667:2673	focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites	2667:2783	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	16	19	from	dispersions	2425:2435	arg1	hand					2364:2367	the other hand	2354:2367	the other hand	2354:2367	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	17	20	theme	rod-shaped	2598:2607	arg1	particles					2609:2617	silica rod-shaped particles	2591:2617	silica rod-shaped particles	2591:2617	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	2	21	theme	self-organized	340:353	arg1	bones					330:334	bones	330:334	bones	330:334	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	21	theme	self-organized	340:353	arg1	composites					373:382	self-organized organic/inorganic composites	340:382	self-organized organic/inorganic composites	340:382	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	21	theme	self-organized	340:353	arg1	teeth					319:323	teeth	319:323	teeth	319:323	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	21	theme	self-organized	340:353	arg1	biominerals					291:301	biominerals	291:301	biominerals such as pearls, teeth, and bones	291:334	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	21	theme	self-organized	340:353	arg1	pearls					311:316	pearls	311:316	pearls	311:316	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	7	22	theme	self-organized	944:957	arg1	structures					972:981	self-organized hierarchical structures	944:981	self-organized hierarchical structures	944:981	Moreover, they form elaborate nanostructures and self-organized hierarchical structures.
35699654	8	23	theme	biological	1168:1177	arg1	tissues					1184:1190	these biological hard tissues	1162:1190	these biological hard tissues	1162:1190	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	17	24	theme	material	2682:2689	arg1	design					2691:2696	material design	2682:2696	material design	2682:2696	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	9	25	theme	components	1398:1407	arg1	combination					1373:1383	the combination	1369:1383	the combination of insoluble components and soluble acidic macromolecules	1369:1441	In biomineralization, the combination of insoluble components and soluble acidic macromolecules controls the crystallization process.
35699654	15	26	used	used	2345:2348	arg2	informational					2300:2312	informational	2300:2312	informational	2300:2312	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	19	27	theme	small-angle	3096:3106	arg1	scattering					3114:3123	small-angle X-ray scattering	3096:3123	small-angle X-ray scattering	3096:3123	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	9	28	theme	soluble	1413:1419	arg1	macromolecules					1428:1441	soluble acidic macromolecules	1413:1441	soluble acidic macromolecules	1413:1441	In biomineralization, the combination of insoluble components and soluble acidic macromolecules controls the crystallization process.
35699654	18	29	theme	new	2879:2881	arg1	materials					2928:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	16	30	theme	other	2358:2362	arg1	hand					2364:2367	the other hand	2354:2367	the other hand	2354:2367	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	15	31	theme	stimuli-responsive	2200:2217	arg1	properties					2219:2228	stimuli-responsive properties	2200:2228	stimuli-responsive properties	2200:2228	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	9	32	theme	macromolecules	1428:1441	arg1	combination					1373:1383	the combination	1369:1383	the combination of insoluble components and soluble acidic macromolecules	1369:1441	In biomineralization, the combination of insoluble components and soluble acidic macromolecules controls the crystallization process.
35699654	20	33	theme	biomineral-inspired	3220:3238	arg1	crystals					3247:3254	these biomineral-inspired liquid crystals	3214:3254	these biomineral-inspired liquid crystals	3214:3254	The functions of these biomineral-inspired liquid crystals are presented.
35699654	18	34	theme	self-organized	2913:2926	arg1	materials					2928:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	16	35	theme	inorganic	2464:2472	arg1	nano-					2474:2478	anisotropic organic and inorganic nano-	2440:2478	anisotropic organic and inorganic nano-	2440:2478	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	17	36	theme	clay	2537:2540	arg1	nanosheets					2542:2551	clay nanosheets	2537:2551	clay nanosheets	2537:2551	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	20	37	theme	crystals	3247:3254	arg1	functions					3201:3209	The functions	3197:3209	The functions of these biomineral-inspired liquid crystals	3197:3254	The functions of these biomineral-inspired liquid crystals are presented.
35699654	12	38	theme	aqueous	1863:1869	arg1	media					1871:1875	aqueous media	1863:1875	aqueous media using a macromolecular acidic additive, PAA	1863:1919	These were obtained in aqueous media using a macromolecular acidic additive, PAA, without using insoluble polymer templates.
35699654	0	39	theme	Hybrids	64:70	arg1	Assembly					52:59	Assembly	52:59	Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components	52:135	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	1	40	theme	organic/inorganic	177:193	arg1	materials					269:277	high-performance and functional materials	237:277	high-performance and functional materials	237:277	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	1	40	theme	organic/inorganic	177:193	arg1	composites					205:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	0	41	theme	Inorganic	83:91	arg1	Nanocrystals					93:104	Inorganic Nanocrystals	83:104	Inorganic Nanocrystals	83:104	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	10	42	theme	film	1598:1601	arg1	crystals					1603:1610	thin film crystals	1593:1610	thin film crystals of CaCO3 or HAp	1593:1626	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	13	43	theme	phases.LC	2082:2090	arg1	materials					2092:2100	colloidal liquid-crystalline (LC) phases.LC materials	2048:2100	colloidal liquid-crystalline (LC) phases.LC materials	2048:2100	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	5	44	theme	higher	664:669	arg1	performances					671:682	significantly higher performances	650:682	significantly higher performances	650:682	Biominerals do not show significantly higher performances than synthetic composites such as glass-fiber- or carbon-fiber-reinforced plastics.
35699654	18	45	theme	biocompatible	2899:2911	arg1	materials					2928:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	0	46	theme	Polymer	118:124	arg1	Components					126:135	Organic Polymer Components	110:135	Organic Polymer Components	110:135	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	8	47	theme	nanostructured	1206:1219	arg1	films					1226:1230	nanostructured thin films and bulk hybrid crystals	1206:1255	films	1226:1230	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	8	48	theme	material	1010:1017	arg1	design					1019:1024	material design	1010:1024	material design	1010:1024	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	17	49	theme	colloidal	2627:2635	arg1	states.In					2640:2648	colloidal LC states.In	2627:2648	colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites	2627:2783	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	13	50	theme	liquid-crystalline	2058:2075	arg1	materials					2092:2100	colloidal liquid-crystalline (LC) phases.LC materials	2048:2100	colloidal liquid-crystalline (LC) phases.LC materials	2048:2100	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	0	51	theme	Biomineral-Inspired	0:18	arg1	Crystals					37:44	Biomineral-Inspired Colloidal Liquid Crystals	0:44	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.	0:163	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	15	52	theme	properties	2219:2228	arg1	addition					2188:2195	The addition	2184:2195	The addition of stimuli-responsive properties to organic rod-like LC molecules	2184:2261	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	15	53	theme	LC	2250:2251	arg1	molecules					2253:2261	organic rod-like LC molecules	2233:2261	organic rod-like LC molecules	2233:2261	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	3	54	theme	calcium	426:432	arg1	CaCO3					445:449	CaCO3	445:449	CaCO3	445:449	The inorganic components are composed of calcium carbonate (CaCO3) and hydroxyapatite (HAp), while the organic components consist of peptides and polysaccharides.
35699654	3	54	theme	calcium	426:432	arg1	carbonate					434:442	calcium carbonate	426:442	calcium carbonate (CaCO3)	426:450	The inorganic components are composed of calcium carbonate (CaCO3) and hydroxyapatite (HAp), while the organic components consist of peptides and polysaccharides.
35699654	10	55	theme	organic	1658:1664	arg1	polymers					1705:1712	chitin and synthetic polymers	1684:1712	chitin and synthetic polymers bearing the OH group	1684:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	10	55	theme	organic	1658:1664	arg1	templates					1666:1674	insoluble organic templates	1648:1674	insoluble organic templates such as chitin and synthetic polymers bearing the OH group	1648:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	13	56	theme	anisotropic	2000:2010	arg1	particles					2012:2020	the anisotropic particles	1996:2020	the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials	1996:2100	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	0	57	theme	Liquid	30:35	arg1	Crystals					37:44	Biomineral-Inspired Colloidal Liquid Crystals	0:44	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.	0:163	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	8	58	theme	bulk	1236:1239	arg1	crystals					1248:1255	nanostructured thin films and bulk hybrid crystals	1206:1255	crystals	1248:1255	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	14	59	theme	mobile	2169:2174	arg1	states					2176:2181	mobile states	2169:2181	mobile states	2169:2181	They show ordered and mobile states.
35699654	8	60	theme	biomineral-based	1037:1052	arg1	composites					1086:1095	these biomineral-based hierarchical and nanostructured composites	1031:1095	these biomineral-based hierarchical and nanostructured composites	1031:1095	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	4	61	theme	biological	606:615	arg1	tissues					617:623	hard biological tissues	601:623	hard biological tissues	601:623	These composites are used as structural materials in hard biological tissues.
35699654	18	62	theme	materials	2928:2936	arg1	nanostructures					2826:2839	nanostructures	2826:2839	nanostructures	2826:2839	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	18	62	theme	materials	2928:2936	arg1	preparation					2813:2823	preparation	2813:2823	preparation	2813:2823	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	18	62	theme	materials	2928:2936	arg1	behavior					2861:2868	self-assembled behavior	2846:2868	self-assembled behavior	2846:2868	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	18	62	theme	materials	2928:2936	arg1	design					2802:2807	design	2802:2807	design	2802:2807	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	19	63	theme	self-assembled	2978:2991	arg1	crystals					3025:3032	these self-assembled nanostructured colloidal liquid crystals	2972:3032	these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering	2972:3147	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	15	64	dep	informational	2300:2312	arg1	displays					2314:2321	displays	2314:2321	displays	2314:2321	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	10	65	theme	chitin	1684:1689	arg1	polymers					1705:1712	chitin and synthetic polymers	1684:1712	chitin and synthetic polymers bearing the OH group	1684:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	17	66	theme	hierarchical	2702:2713	arg1	aspects					2715:2721	hierarchical aspects	2702:2721	hierarchical aspects	2702:2721	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	4	67	theme	structural	577:586	arg1	materials					588:596	structural materials	577:596	structural materials in hard biological tissues	577:623	These composites are used as structural materials in hard biological tissues.
35699654	4	67	theme	structural	577:586	arg1	composites					554:563	These composites	548:563	These composites	548:563	These composites are used as structural materials in hard biological tissues.
35699654	21	68	theme	biomineral-based	3285:3300	arg1	crystals					3322:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	10	69	theme	acrylic	1505:1511	arg1	poly					1500:1503	poly	1500:1503	poly(acrylic acid) (PAA)	1500:1523	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	10	69	theme	acrylic	1505:1511	arg1	acid					1513:1516	acrylic acid	1505:1516	acrylic acid	1505:1516	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	17	70	dep	design	2691:2696	arg1	the					2678:2680	the	2678:2680	the	2678:2680	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	15	71	theme	informational	2300:2312	arg1	development					2285:2295	the successful development	2270:2295	the successful development of informational displays, which are now widely used	2270:2348	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	12	72	theme	polymer	1946:1952	arg1	templates					1954:1962	insoluble polymer templates	1936:1962	insoluble polymer templates	1936:1962	These were obtained in aqueous media using a macromolecular acidic additive, PAA, without using insoluble polymer templates.
35699654	19	73	theme	rheological	3153:3163	arg1	measurements					3165:3176	rheological measurements	3153:3176	rheological measurements	3153:3176	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	16	74	theme	colloidal	2370:2378	arg1	crystals					2387:2394	colloidal liquid crystals	2370:2394	colloidal liquid crystals	2370:2394	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	16	74	theme	colloidal	2370:2378	arg1	dispersions					2425:2435	colloidal self-assembled dispersions	2400:2435	colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects	2400:2496	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	21	75	theme	colloidal	3305:3313	arg1	crystals					3322:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	11	76	theme	HAp-based	1779:1787	arg1	particles					1804:1812	CaCO3- and HAp-based nanostructured particles	1768:1812	CaCO3- and HAp-based nanostructured particles with rod and disk shapes	1768:1837	Moreover, we recently developed CaCO3- and HAp-based nanostructured particles with rod and disk shapes.
35699654	15	77	theme	organic	2233:2239	arg1	molecules					2253:2261	organic rod-like LC molecules	2233:2261	organic rod-like LC molecules	2233:2261	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	8	78	theme	polymers	1298:1305	arg1	self-organization					1269:1285	the self-organization	1265:1285	the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp	1265:1344	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	19	79	theme	colloidal	3008:3016	arg1	crystals					3025:3032	these self-assembled nanostructured colloidal liquid crystals	2972:3032	these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering	2972:3147	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	17	80	theme	colloidal	2743:2751	arg1	composites					2774:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	17	81	theme	plate-shaped	2563:2574	arg1	particles					2576:2584	gibbsite plate-shaped particles	2554:2584	gibbsite plate-shaped particles	2554:2584	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	10	82	theme	synthetic	1695:1703	arg1	polymers					1705:1712	chitin and synthetic polymers	1684:1712	chitin and synthetic polymers bearing the OH group	1684:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	11	83	theme	CaCO3-	1768:1773	arg1	particles					1804:1812	CaCO3- and HAp-based nanostructured particles	1768:1812	CaCO3- and HAp-based nanostructured particles with rod and disk shapes	1768:1837	Moreover, we recently developed CaCO3- and HAp-based nanostructured particles with rod and disk shapes.
35699654	11	84	with	particles	1804:1812	arg1	rod					1819:1821	rod	1819:1821	rod	1819:1821	Moreover, we recently developed CaCO3- and HAp-based nanostructured particles with rod and disk shapes.
35699654	11	84	with	particles	1804:1812	arg1	shapes					1832:1837	disk shapes	1827:1837	disk shapes	1827:1837	Moreover, we recently developed CaCO3- and HAp-based nanostructured particles with rod and disk shapes.
35699654	8	85	theme	inorganic	1311:1319	arg1	crystals					1321:1328	inorganic crystals	1311:1328	inorganic crystals	1311:1328	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	7	86	dep	form	910:913	arg1	elaborate					915:923	elaborate	915:923	form elaborate nanostructures and self-organized hierarchical structures	910:981	Moreover, they form elaborate nanostructures and self-organized hierarchical structures.
35699654	17	87	theme	polymer/inorganic	2756:2772	arg1	composites					2774:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	16	88	theme	colloidal	2400:2408	arg1	crystals					2387:2394	colloidal liquid crystals	2370:2394	colloidal liquid crystals	2370:2394	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	16	88	theme	colloidal	2400:2408	arg1	dispersions					2425:2435	colloidal self-assembled dispersions	2400:2435	colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects	2400:2496	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	18	89	theme	bioinspired	2883:2893	arg1	materials					2928:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	these new bioinspired and biocompatible self-organized materials	2873:2936	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	10	90	theme	polymer	1551:1557	arg1	additives					1559:1567	called polymer additives	1544:1567	called polymer additives	1544:1567	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	8	91	theme	materials.Inspired	1140:1157	arg1	preparation					1114:1124	the preparation	1110:1124	the preparation of functional materials.Inspired by these biological hard tissues	1110:1190	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	19	92	theme	neutron	3130:3136	arg1	scattering					3138:3147	neutron scattering	3130:3147	neutron scattering	3130:3147	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	17	93	theme	silica	2591:2596	arg1	particles					2609:2617	silica rod-shaped particles	2591:2617	silica rod-shaped particles	2591:2617	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	10	94	theme	OH	1726:1727	arg1	group					1729:1733	the OH group	1722:1733	the OH group	1722:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	19	95	theme	high-resolution	3046:3060	arg1	microscopy					3084:3093	high-resolution transmission electron microscopy	3046:3093	high-resolution transmission electron microscopy	3046:3093	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	11	96	theme	disk	1827:1830	arg1	shapes					1832:1837	disk shapes	1827:1837	disk shapes	1827:1837	Moreover, we recently developed CaCO3- and HAp-based nanostructured particles with rod and disk shapes.
35699654	4	97	used	used	569:572	arg2	composites					554:563	These composites	548:563	These composites	548:563	These composites are used as structural materials in hard biological tissues.
35699654	4	97	used	used	569:572	arg2	materials					588:596	structural materials	577:596	structural materials in hard biological tissues	577:623	These composites are used as structural materials in hard biological tissues.
35699654	13	98	theme	organic	2128:2134	arg1	molecules					2136:2144	organic molecules	2128:2144	organic molecules	2128:2144	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	2	99	theme	organic/inorganic	355:371	arg1	bones					330:334	bones	330:334	bones	330:334	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	99	theme	organic/inorganic	355:371	arg1	composites					373:382	self-organized organic/inorganic composites	340:382	self-organized organic/inorganic composites	340:382	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	99	theme	organic/inorganic	355:371	arg1	teeth					319:323	teeth	319:323	teeth	319:323	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	99	theme	organic/inorganic	355:371	arg1	biominerals					291:301	biominerals	291:301	biominerals such as pearls, teeth, and bones	291:334	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	2	99	theme	organic/inorganic	355:371	arg1	pearls					311:316	pearls	311:316	pearls	311:316	In nature, biominerals such as pearls, teeth, and bones are self-organized organic/inorganic composites.
35699654	8	100	theme	hard	1179:1182	arg1	tissues					1184:1190	these biological hard tissues	1162:1190	these biological hard tissues	1162:1190	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	9	101	theme	insoluble	1388:1396	arg1	components					1398:1407	insoluble components	1388:1407	insoluble components	1388:1407	In biomineralization, the combination of insoluble components and soluble acidic macromolecules controls the crystallization process.
35699654	21	102	theme	environmentally	3401:3415	arg1	friendly					3417:3424	environmentally friendly	3401:3424	environmentally friendly	3401:3424	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	7	103	theme	hierarchical	959:970	arg1	structures					972:981	self-organized hierarchical structures	944:981	self-organized hierarchical structures	944:981	Moreover, they form elaborate nanostructures and self-organized hierarchical structures.
35699654	1	104	theme	high-performance	237:252	arg1	composites					205:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	1	104	theme	high-performance	237:252	arg1	materials					269:277	high-performance and functional materials	237:277	high-performance and functional materials	237:277	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	1	105	theme	functional	258:267	arg1	composites					205:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	1	105	theme	functional	258:267	arg1	materials					269:277	high-performance and functional materials	237:277	high-performance and functional materials	237:277	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	9	106	theme	acidic	1421:1426	arg1	macromolecules					1428:1441	soluble acidic macromolecules	1413:1441	soluble acidic macromolecules	1413:1441	In biomineralization, the combination of insoluble components and soluble acidic macromolecules controls the crystallization process.
35699654	17	107	theme	polysaccharide	2512:2525	arg1	whiskers					2527:2534	polysaccharide whiskers	2512:2534	polysaccharide whiskers	2512:2534	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	6	108	theme	mild	878:881	arg1	conditions					883:892	mild conditions	878:892	mild conditions	878:892	However, biominerals consist of environmentally friendly and biocompatible components that are prepared under mild conditions.
35699654	19	109	theme	liquid	3018:3023	arg1	crystals					3025:3032	these self-assembled nanostructured colloidal liquid crystals	2972:3032	these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering	2972:3147	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	21	110	theme	new	3456:3458	arg1	functions					3460:3468	new functions	3456:3468	new functions	3456:3468	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	10	111	dep	poly	1500:1503	arg1	additives					1559:1567	called polymer additives	1544:1567	called polymer additives	1544:1567	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	19	112	theme	X-ray	3108:3112	arg1	scattering					3114:3123	small-angle X-ray scattering	3096:3123	small-angle X-ray scattering	3096:3123	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	10	113	with	cooperation	1631:1641	arg1	polymers					1705:1712	chitin and synthetic polymers	1684:1712	chitin and synthetic polymers bearing the OH group	1684:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	10	113	with	cooperation	1631:1641	arg1	templates					1666:1674	insoluble organic templates	1648:1674	insoluble organic templates such as chitin and synthetic polymers bearing the OH group	1648:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	9	114	theme	crystallization	1456:1470	arg1	process					1472:1478	the crystallization process	1452:1478	the crystallization process	1452:1478	In biomineralization, the combination of insoluble components and soluble acidic macromolecules controls the crystallization process.
35699654	1	115	theme	Bioinspired	165:175	arg1	materials					269:277	high-performance and functional materials	237:277	high-performance and functional materials	237:277	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	1	115	theme	Bioinspired	165:175	arg1	composites					205:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	3	116	theme	inorganic	389:397	arg1	components					399:408	The inorganic components	385:408	The inorganic components	385:408	The inorganic components are composed of calcium carbonate (CaCO3) and hydroxyapatite (HAp), while the organic components consist of peptides and polysaccharides.
35699654	20	117	theme	liquid	3240:3245	arg1	crystals					3247:3254	these biomineral-inspired liquid crystals	3214:3254	these biomineral-inspired liquid crystals	3214:3254	The functions of these biomineral-inspired liquid crystals are presented.
35699654	1	118	theme	synthetic	195:203	arg1	materials					269:277	high-performance and functional materials	237:277	high-performance and functional materials	237:277	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	1	118	theme	synthetic	195:203	arg1	composites					205:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites	165:214	Bioinspired organic/inorganic synthetic composites have been studied as high-performance and functional materials.
35699654	10	119	theme	crystals	1603:1610	arg1	formation					1580:1588	the formation	1576:1588	the formation of thin film crystals of CaCO3 or HAp	1576:1626	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	0	120	theme	Organic	110:116	arg1	Components					126:135	Organic Polymer Components	110:135	Organic Polymer Components	110:135	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	13	121	dep	particles	2012:2020	arg1	formed					2041:2046	formed	2041:2046	formed colloidal liquid-crystalline (LC) phases.LC materials	2041:2100	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	13	121	dep	particles	2012:2020	arg1	self-assembled					2022:2035	self-assembled	2022:2035	self-assembled	2022:2035	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	10	122	theme	thin	1593:1596	arg1	crystals					1603:1610	thin film crystals	1593:1610	thin film crystals of CaCO3 or HAp	1593:1626	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	6	123	theme	environmentally	800:814	arg1	friendly					816:823	environmentally friendly	800:823	environmentally friendly	800:823	However, biominerals consist of environmentally friendly and biocompatible components that are prepared under mild conditions.
35699654	0	124	theme	Components	126:135	arg1	Assembly					52:59	Assembly	52:59	Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components	52:135	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	16	125	theme	anisotropic	2440:2450	arg1	nano-					2474:2478	anisotropic organic and inorganic nano-	2440:2478	anisotropic organic and inorganic nano-	2440:2478	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	8	126	theme	thin	1221:1224	arg1	films					1226:1230	nanostructured thin films and bulk hybrid crystals	1206:1255	films	1226:1230	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	10	127	theme	HAp	1624:1626	arg1	crystals					1603:1610	thin film crystals	1593:1610	thin film crystals of CaCO3 or HAp	1593:1626	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	13	128	theme	colloidal	2048:2056	arg1	LC					2078:2079	LC	2078:2079	LC	2078:2079	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	13	128	theme	colloidal	2048:2056	arg1	liquid-crystalline					2058:2075	colloidal liquid-crystalline	2048:2075	colloidal liquid-crystalline (LC) phases.LC materials	2048:2100	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	0	129	theme	Colloidal	20:28	arg1	Crystals					37:44	Biomineral-Inspired Colloidal Liquid Crystals	0:44	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.	0:163	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	18	130	theme	self-assembled	2846:2859	arg1	behavior					2861:2868	self-assembled behavior	2846:2868	self-assembled behavior	2846:2868	We describe the design and preparation, nanostructures, and self-assembled behavior of these new bioinspired and biocompatible self-organized materials.
35699654	15	131	theme	rod-like	2241:2248	arg1	molecules					2253:2261	organic rod-like LC molecules	2233:2261	organic rod-like LC molecules	2233:2261	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	4	132	theme	hard	601:604	arg1	tissues					617:623	hard biological tissues	601:623	hard biological tissues	601:623	These composites are used as structural materials in hard biological tissues.
35699654	10	133	theme	CaCO3	1615:1619	arg1	crystals					1603:1610	thin film crystals	1593:1610	thin film crystals of CaCO3 or HAp	1593:1626	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	16	134	theme	nano-	2474:2478	arg1	crystals					2387:2394	colloidal liquid crystals	2370:2394	colloidal liquid crystals	2370:2394	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	16	134	theme	nano-	2474:2478	arg1	dispersions					2425:2435	colloidal self-assembled dispersions	2400:2435	colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects	2400:2496	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	19	135	theme	nanostructured	2993:3006	arg1	crystals					3025:3032	these self-assembled nanostructured colloidal liquid crystals	2972:3032	these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering	2972:3147	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	8	136	theme	hybrid	1241:1246	arg1	crystals					1248:1255	nanostructured thin films and bulk hybrid crystals	1206:1255	crystals	1248:1255	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	6	137	theme	biocompatible	829:841	arg1	components					843:852	biocompatible components	829:852	biocompatible components	829:852	However, biominerals consist of environmentally friendly and biocompatible components that are prepared under mild conditions.
35699654	10	138	theme	insoluble	1648:1656	arg1	polymers					1705:1712	chitin and synthetic polymers	1684:1712	chitin and synthetic polymers bearing the OH group	1684:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	10	138	theme	insoluble	1648:1656	arg1	templates					1666:1674	insoluble organic templates	1648:1674	insoluble organic templates such as chitin and synthetic polymers bearing the OH group	1648:1733	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	21	139	theme	liquid	3315:3320	arg1	crystals					3322:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	16	140	theme	micro-objects	2484:2496	arg1	crystals					2387:2394	colloidal liquid crystals	2370:2394	colloidal liquid crystals	2370:2394	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	16	140	theme	micro-objects	2484:2496	arg1	dispersions					2425:2435	colloidal self-assembled dispersions	2400:2435	colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects	2400:2496	On the other hand, colloidal liquid crystals are colloidal self-assembled dispersions of anisotropic organic and inorganic nano- and micro-objects.
35699654	4	141	from	materials	588:596	arg1	tissues					617:623	hard biological tissues	601:623	hard biological tissues	601:623	These composites are used as structural materials in hard biological tissues.
35699654	19	142	theme	electron	3075:3082	arg1	microscopy					3084:3093	high-resolution transmission electron microscopy	3046:3093	high-resolution transmission electron microscopy	3046:3093	The characterization results for these self-assembled nanostructured colloidal liquid crystals found using high-resolution transmission electron microscopy, small-angle X-ray scattering, and neutron scattering and rheological measurements are also reported.
35699654	17	143	theme	LC	2637:2638	arg1	states.In					2640:2648	colloidal LC states.In	2627:2648	colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites	2627:2783	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	3	144	theme	organic	488:494	arg1	components					496:505	the organic components	484:505	the organic components	484:505	The inorganic components are composed of calcium carbonate (CaCO3) and hydroxyapatite (HAp), while the organic components consist of peptides and polysaccharides.
35699654	8	145	theme	hierarchical	1054:1065	arg1	composites					1086:1095	these biomineral-based hierarchical and nanostructured composites	1031:1095	these biomineral-based hierarchical and nanostructured composites	1031:1095	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	8	146	theme	organic	1290:1296	arg1	polymers					1298:1305	organic polymers	1290:1305	organic polymers	1290:1305	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	0	147	dep	Crystals	37:44	arg1	Assembly					52:59	Assembly	52:59	Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components	52:135	Biomineral-Inspired Colloidal Liquid Crystals: From Assembly of Hybrids Comprising Inorganic Nanocrystals and Organic Polymer Components to Their Functionalization.
35699654	8	148	theme	nanostructured	1071:1084	arg1	composites					1086:1095	these biomineral-based hierarchical and nanostructured composites	1031:1095	these biomineral-based hierarchical and nanostructured composites	1031:1095	Much can be learned about material design from these biomineral-based hierarchical and nanostructured composites to assist in the preparation of functional materials.Inspired by these biological hard tissues, we developed nanostructured thin films and bulk hybrid crystals through the self-organization of organic polymers and inorganic crystals of CaCO3 or HAp.
35699654	21	149	theme	LC	3302:3303	arg1	crystals					3322:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	these biomineral-based LC colloidal liquid crystals	3279:3329	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35699654	10	150	theme	acidic	1528:1533	arg1	peptides					1535:1542	acidic peptides	1528:1542	acidic peptides	1528:1542	We have shown that poly(acrylic acid) (PAA) or acidic peptides called polymer additives induce the formation of thin film crystals of CaCO3 or HAp by cooperation with insoluble organic templates such as chitin and synthetic polymers bearing the OH group.
35699654	15	151	theme	successful	2274:2283	arg1	development					2285:2295	the successful development	2270:2295	the successful development of informational displays, which are now widely used	2270:2348	The addition of stimuli-responsive properties to organic rod-like LC molecules led to the successful development of informational displays, which are now widely used.
35699654	12	152	theme	insoluble	1936:1944	arg1	templates					1954:1962	insoluble polymer templates	1936:1962	insoluble polymer templates	1936:1962	These were obtained in aqueous media using a macromolecular acidic additive, PAA, without using insoluble polymer templates.
35699654	17	153	theme	biomineral-based	2726:2741	arg1	composites					2774:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	biomineral-based colloidal LC polymer/inorganic composites	2726:2783	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	17	154	theme	gibbsite	2554:2561	arg1	particles					2576:2584	gibbsite plate-shaped particles	2554:2584	gibbsite plate-shaped particles	2554:2584	For example, polysaccharide whiskers, clay nanosheets, gibbsite plate-shaped particles, and silica rod-shaped particles exhibit colloidal LC states.In this Account, we focused on the material design and hierarchical aspects of biomineral-based colloidal LC polymer/inorganic composites.
35699654	13	155	theme	appropriate	1968:1978	arg1	concentrations					1980:1993	appropriate concentrations	1968:1993	appropriate concentrations	1968:1993	At appropriate concentrations, the anisotropic particles self-assembled and formed colloidal liquid-crystalline (LC) phases.LC materials are generally composed of organic molecules.
35699654	21	156	theme	aqueous	3362:3368	arg1	conditions					3370:3379	mild and aqueous conditions	3353:3379	mild and aqueous conditions	3353:3379	Because these biomineral-based LC colloidal liquid crystals can be prepared under mild and aqueous conditions and they consist of environmentally friendly and biocompatible components, new functions are expected for these materials.
35297435	4	0	theme	barrier	853:859	arg1	modulation					861:870	mucus barrier modulation	847:870	mucus barrier modulation	847:870	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	1	theme	anti-inflammatory	575:591	arg1	acid					621:624	docosahexaenoic acid	605:624	docosahexaenoic acid (DHA)	605:630	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	1	theme	anti-inflammatory	575:591	arg1	acids					599:603	the anti-inflammatory fatty acids	571:603	the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)	571:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	1	theme	anti-inflammatory	575:591	arg1	acid					653:656	eicosapentaenoic acid	636:656	eicosapentaenoic acid (EPA)	636:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	2	from	progression	670:680	arg1	mice					685:688	mice	685:688	mice	685:688	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	9	3	theme	reinforced	1596:1605	arg1	layer					1613:1617	a reinforced mucus layer	1594:1617	a reinforced mucus layer	1594:1617	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	4	4	theme	acids	599:603	arg1	effects					560:566	Differential effects	547:566	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice	547:688	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	5	theme	previous	720:727	arg1	work					729:732	our previous work	716:732	our previous work	716:732	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	6	6	theme	qPCR	1232:1235	arg1	combination					1166:1176	a combination	1164:1176	a combination of electron microscopy, specific mucous staining, and qPCR	1164:1235	Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR.
35297435	1	7	theme	colon	239:243	arg1	colitis					184:190	Ulcerative colitis	173:190	Ulcerative colitis (UC)	173:195	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	7	theme	colon	239:243	arg1	Background					161:170	Background	161:170	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.	161:295	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	7	theme	colon	239:243	arg1	disorder					223:230	a chronic inflammatory disorder	200:230	a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course	200:294	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	5	8	with	mice	996:999	arg1	colitis					1043:1049	dextran sodium sulfate (DSS)-induced colitis	1006:1049	dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA	1006:1097	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	10	9	theme	gut	1882:1884	arg1	microbiome					1886:1895	the gut microbiome	1878:1895	the gut microbiome	1878:1895	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	6	10	theme	mucous	1211:1216	arg1	staining					1218:1225	specific mucous staining	1202:1225	specific mucous staining	1202:1225	Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR.
35297435	0	11	from	colitis	106:112	arg1	mice					117:120	mice	117:120	mice	117:120	EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.
35297435	1	12	theme	Ulcerative	173:182	arg1	UC					193:194	UC	193:194	UC	193:194	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	12	theme	Ulcerative	173:182	arg1	disorder					223:230	a chronic inflammatory disorder	200:230	a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course	200:294	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	12	theme	Ulcerative	173:182	arg1	colitis					184:190	Ulcerative colitis	173:190	Ulcerative colitis (UC)	173:195	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	12	theme	Ulcerative	173:182	arg1	Background					161:170	Background	161:170	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.	161:295	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	9	13	theme	increased	1635:1643	arg1	release					1659:1665	increased mucin granule release	1635:1665	increased mucin granule release	1635:1665	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	4	14	dep	acids	599:603	arg1	acids					599:603	the anti-inflammatory fatty acids	571:603	the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)	571:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	14	dep	acids	599:603	arg1	acid					621:624	docosahexaenoic acid	605:624	docosahexaenoic acid (DHA)	605:630	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	14	dep	acids	599:603	arg1	acid					653:656	eicosapentaenoic acid	636:656	eicosapentaenoic acid (EPA)	636:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	14	dep	acids	599:603	arg1	EPA					659:661	EPA	659:661	EPA	659:661	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	14	dep	acids	599:603	arg1	DHA					627:629	DHA	627:629	DHA	627:629	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	9	15	theme	granule	1651:1657	arg1	release					1659:1665	increased mucin granule release	1635:1665	increased mucin granule release	1635:1665	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	6	16	theme	specific	1202:1209	arg1	staining					1218:1225	specific mucous staining	1202:1225	specific mucous staining	1202:1225	Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR.
35297435	6	17	theme	microscopy	1190:1199	arg1	combination					1166:1176	a combination	1164:1176	a combination of electron microscopy, specific mucous staining, and qPCR	1164:1235	Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR.
35297435	0	18	theme	reinforced	127:136	arg1	barrier					152:158	a reinforced colonic mucus barrier	125:158	a reinforced colonic mucus barrier	125:158	EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.
35297435	9	19	theme	layer	1674:1678	arg1	stratification					1680:1693	mucus layer stratification	1668:1693	mucus layer stratification	1668:1693	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	8	20	theme	16S	1431:1433	arg1	sequencing					1440:1449	16S rRNA sequencing	1431:1449	16S rRNA sequencing	1431:1449	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	10	21	theme	mucin-degrading	1972:1986	arg1	bacterium					1988:1996	mucin-degrading bacterium	1972:1996	mucin-degrading bacterium	1972:1996	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	0	22	theme	mucus	146:150	arg1	barrier					152:158	a reinforced colonic mucus barrier	125:158	a reinforced colonic mucus barrier	125:158	EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.
35297435	10	23	theme	Bacteroidetes	1954:1966	arg1	abundance					1930:1938	the enriched abundance	1917:1938	the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids	1917:2057	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	5	24	theme	EPA	1087:1089	arg1	doses					1078:1082	different doses	1068:1082	different doses of EPA and DHA	1068:1097	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	9	25	from	effect	1507:1512	arg1	colitis					1546:1552	DSS-induced colitis	1534:1552	DSS-induced colitis	1534:1552	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	9	26	theme	upregulated	1708:1718	arg1	expression					1720:1729	markedly upregulated expression	1699:1729	markedly upregulated expression	1699:1729	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	12	27	theme	adjunctive	2261:2270	arg1	therapy					2272:2278	a promising adjunctive therapy	2249:2278	a promising adjunctive therapy for UC	2249:2285	EPA may develop into a promising adjunctive therapy for UC.
35297435	0	28	theme	gut	69:71	arg1	microbiota					73:82	gut microbiota	69:82	gut microbiota	69:82	EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.
35297435	5	29	theme	DHA	1095:1097	arg1	doses					1078:1082	different doses	1068:1082	different doses of EPA and DHA	1068:1097	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	8	30	theme	Fecal	1308:1312	arg1	acids					1332:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	5	31	theme	activity	939:946	arg1	index					948:952	the disease activity index	927:952	the disease activity index	927:952	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	9	32	theme	key	1738:1740	arg1	modulators					1742:1751	the key modulators	1734:1751	the key modulators involved in goblet cell differentiation	1734:1791	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	8	33	theme	chain	1320:1324	arg1	acids					1332:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	0	34	theme	DSS-induced	94:104	arg1	colitis					106:112	DSS-induced colitis	94:112	DSS-induced colitis in mice by a reinforced colonic mucus barrier	94:158	EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.
35297435	7	35	used	used	1259:1262	arg2	blotting					1246:1253	Western blotting	1238:1253	Western blotting	1238:1253	Western blotting was used to analyze the underlying molecular events.
35297435	3	36	theme	barrier	397:403	arg1	necessary					442:450	necessary	442:450	necessary	442:450	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	3	36	theme	barrier	397:403	arg1	lining					405:410	The mucus barrier lining the gastrointestinal tract	387:437	The mucus barrier lining the gastrointestinal tract	387:437	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	4	37	from	effects	560:566	arg1	progression					670:680	UC progression	667:680	UC progression in mice	667:688	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	9	38	theme	dose	1522:1525	arg1	EPA					1527:1529	high dose EPA	1517:1529	high dose EPA	1517:1529	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	7	39	theme	underlying	1279:1288	arg1	events					1300:1305	the underlying molecular events	1275:1305	the underlying molecular events	1275:1305	Western blotting was used to analyze the underlying molecular events.
35297435	9	40	theme	goblet	1765:1770	arg1	differentiation					1777:1791	goblet cell differentiation	1765:1791	goblet cell differentiation	1765:1791	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	10	41	theme	enhanced	1815:1822	arg1	barrier					1830:1836	a remarkably enhanced mucus barrier	1802:1836	a remarkably enhanced mucus barrier in the EPA group	1802:1853	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	8	42	theme	gas	1358:1360	arg1	chromatography					1362:1375	gas chromatography	1358:1375	gas chromatography	1358:1375	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	11	43	theme	mucus	2179:2183	arg1	disruption					2193:2202	mucus barrier disruption	2179:2202	mucus barrier disruption in DSS-induced colitis	2179:2225	Conclusions: EPA and DHA differentially coordinate the interaction between the host and the gut microbiota and relieve mucus barrier disruption in DSS-induced colitis.
35297435	3	44	dep	lining	405:410	arg1	tract					433:437	the gastrointestinal tract	412:437	The mucus barrier lining the gastrointestinal tract	387:437	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	1	45	with	disorder	223:230	arg1	course					289:294	a continuously remitting and relapsing course	250:294	a continuously remitting and relapsing course	250:294	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	11	46	from	disruption	2193:2202	arg1	colitis					2219:2225	DSS-induced colitis	2207:2225	DSS-induced colitis	2207:2225	Conclusions: EPA and DHA differentially coordinate the interaction between the host and the gut microbiota and relieve mucus barrier disruption in DSS-induced colitis.
35297435	2	47	dep	host	362:365	arg1	microbiota					375:384	microbiota	375:384	microbiota	375:384	Its etiology is closely related to abnormal interactions between host and gut microbiota.
35297435	5	48	theme	score	973:977	arg1	Assessment					913:922	Assessment	913:922	Assessment of the disease activity index and histopathology score	913:977	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	4	49	theme	docosahexaenoic	605:619	arg1	acid					621:624	docosahexaenoic acid	605:624	docosahexaenoic acid (DHA)	605:630	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	49	theme	docosahexaenoic	605:619	arg1	acids					599:603	the anti-inflammatory fatty acids	571:603	the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)	571:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	49	theme	docosahexaenoic	605:619	arg1	DHA					627:629	DHA	627:629	DHA	627:629	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	8	50	theme	microbial	1390:1398	arg1	composition					1400:1410	the gut microbial composition	1382:1410	the gut microbial composition	1382:1410	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	1	51	theme	chronic	202:208	arg1	colitis					184:190	Ulcerative colitis	173:190	Ulcerative colitis (UC)	173:195	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	51	theme	chronic	202:208	arg1	Background					161:170	Background	161:170	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.	161:295	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	51	theme	chronic	202:208	arg1	disorder					223:230	a chronic inflammatory disorder	200:230	a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course	200:294	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	11	52	theme	barrier	2185:2191	arg1	disruption					2193:2202	mucus barrier disruption	2179:2202	mucus barrier disruption in DSS-induced colitis	2179:2225	Conclusions: EPA and DHA differentially coordinate the interaction between the host and the gut microbiota and relieve mucus barrier disruption in DSS-induced colitis.
35297435	3	53	theme	microbiota	479:488	arg1	interaction					490:500	host and gut microbiota interaction	466:500	interaction	490:500	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	4	54	theme	mucus	847:851	arg1	modulation					861:870	mucus barrier modulation	847:870	mucus barrier modulation	847:870	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	10	55	from	barrier	1830:1836	arg1	group					1849:1853	the EPA group	1841:1853	the EPA group	1841:1853	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	9	56	theme	mucus	1607:1611	arg1	layer					1613:1617	a reinforced mucus layer	1594:1617	a reinforced mucus layer	1594:1617	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	4	57	theme	fatty	593:597	arg1	acid					621:624	docosahexaenoic acid	605:624	docosahexaenoic acid (DHA)	605:630	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	57	theme	fatty	593:597	arg1	acids					599:603	the anti-inflammatory fatty acids	571:603	the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)	571:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	57	theme	fatty	593:597	arg1	acid					653:656	eicosapentaenoic acid	636:656	eicosapentaenoic acid (EPA)	636:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	58	theme	host-microbiome	812:826	arg1	crosstalk					828:836	host-microbiome crosstalk	812:836	host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy	812:901	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	6	59	theme	staining	1218:1225	arg1	combination					1166:1176	a combination	1164:1176	a combination of electron microscopy, specific mucous staining, and qPCR	1164:1235	Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR.
35297435	4	60	theme	UC	892:893	arg1	therapy					895:901	UC therapy	892:901	UC therapy	892:901	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	6	61	theme	Mucin	1100:1104	arg1	generation					1106:1115	Mucin generation	1100:1115	Mucin generation	1100:1115	Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR.
35297435	5	62	theme	-induced	1034:1041	arg1	colitis					1043:1049	dextran sodium sulfate (DSS)-induced colitis	1006:1049	dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA	1006:1097	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	4	63	theme	eicosapentaenoic	636:651	arg1	acids					599:603	the anti-inflammatory fatty acids	571:603	the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)	571:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	63	theme	eicosapentaenoic	636:651	arg1	EPA					659:661	EPA	659:661	EPA	659:661	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	4	63	theme	eicosapentaenoic	636:651	arg1	acid					653:656	eicosapentaenoic acid	636:656	eicosapentaenoic acid (EPA)	636:662	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	0	64	theme	colonic	138:144	arg1	barrier					152:158	a reinforced colonic mucus barrier	125:158	a reinforced colonic mucus barrier	125:158	EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.
35297435	9	65	theme	mucus	1668:1672	arg1	stratification					1680:1693	mucus layer stratification	1668:1693	mucus layer stratification	1668:1693	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	8	66	theme	rRNA	1435:1438	arg1	sequencing					1440:1449	16S rRNA sequencing	1431:1449	16S rRNA sequencing	1431:1449	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	6	67	theme	electron	1181:1188	arg1	microscopy					1190:1199	electron microscopy	1181:1199	electron microscopy	1181:1199	Mucin generation, glycosylation and secretion were evaluated by a combination of electron microscopy, specific mucous staining, and qPCR.
35297435	10	68	theme	enriched	1921:1928	arg1	abundance					1930:1938	the enriched abundance	1917:1938	the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids	1917:2057	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	5	69	theme	disease	931:937	arg1	index					948:952	the disease activity index	927:952	the disease activity index	927:952	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	10	70	dep	Bacteroidetes	1954:1966	arg1	muciniphila					2010:2020	Akkermansia muciniphila	1998:2020	Akkermansia muciniphila	1998:2020	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	10	70	dep	Bacteroidetes	1954:1966	arg1	the					1943:1945	the	1943:1945	the	1943:1945	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	11	71	theme	DSS-induced	2207:2217	arg1	colitis					2219:2225	DSS-induced colitis	2207:2225	DSS-induced colitis	2207:2225	Conclusions: EPA and DHA differentially coordinate the interaction between the host and the gut microbiota and relieve mucus barrier disruption in DSS-induced colitis.
35297435	4	72	theme	UC	667:668	arg1	progression					670:680	UC progression	667:680	UC progression in mice	667:688	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	9	73	theme	mucin	1645:1649	arg1	release					1659:1665	increased mucin granule release	1635:1665	increased mucin granule release	1635:1665	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	9	74	theme	potent	1489:1494	arg1	effect					1507:1512	the more potent inhibitory effect	1480:1512	the more potent inhibitory effect of high dose EPA on DSS-induced colitis	1480:1552	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	9	75	theme	inhibitory	1496:1505	arg1	effect					1507:1512	the more potent inhibitory effect	1480:1512	the more potent inhibitory effect of high dose EPA on DSS-induced colitis	1480:1552	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	10	76	theme	bacterium	1988:1996	arg1	abundance					1930:1938	the enriched abundance	1917:1938	the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids	1917:2057	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	5	77	theme	different	1068:1076	arg1	doses					1078:1082	different doses	1068:1082	different doses of EPA and DHA	1068:1097	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	5	78	theme	index	948:952	arg1	Assessment					913:922	Assessment	913:922	Assessment of the disease activity index and histopathology score	913:977	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	7	79	theme	molecular	1290:1298	arg1	events					1300:1305	the underlying molecular events	1275:1305	the underlying molecular events	1275:1305	Western blotting was used to analyze the underlying molecular events.
35297435	9	80	theme	modulators	1742:1751	arg1	stratification					1680:1693	mucus layer stratification	1668:1693	mucus layer stratification	1668:1693	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	9	80	theme	modulators	1742:1751	arg1	release					1659:1665	increased mucin granule release	1635:1665	increased mucin granule release	1635:1665	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	9	80	theme	modulators	1742:1751	arg1	expression					1720:1729	markedly upregulated expression	1699:1729	markedly upregulated expression	1699:1729	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	12	81	theme	promising	2251:2259	arg1	therapy					2272:2278	a promising adjunctive therapy	2249:2278	a promising adjunctive therapy for UC	2249:2285	EPA may develop into a promising adjunctive therapy for UC.
35297435	8	82	theme	short	1314:1318	arg1	acids					1332:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	3	83	theme	gastrointestinal	416:431	arg1	tract					433:437	the gastrointestinal tract	412:437	The mucus barrier lining the gastrointestinal tract	387:437	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	9	84	theme	high	1517:1520	arg1	EPA					1527:1529	high dose EPA	1517:1529	high dose EPA	1517:1529	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	11	85	theme	gut	2152:2154	arg1	microbiota					2156:2165	the gut microbiota	2148:2165	the gut microbiota	2148:2165	Conclusions: EPA and DHA differentially coordinate the interaction between the host and the gut microbiota and relieve mucus barrier disruption in DSS-induced colitis.
35297435	4	86	theme	Differential	547:558	arg1	effects					560:566	Differential effects	547:566	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice	547:688	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	8	87	theme	fatty	1326:1330	arg1	acids					1332:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids	1308:1336	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	9	88	theme	EPA	1527:1529	arg1	effect					1507:1512	the more potent inhibitory effect	1480:1512	the more potent inhibitory effect of high dose EPA on DSS-induced colitis	1480:1552	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	9	89	theme	cell	1772:1775	arg1	differentiation					1777:1791	goblet cell differentiation	1765:1791	goblet cell differentiation	1765:1791	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	10	90	theme	mucus	1824:1828	arg1	barrier					1830:1836	a remarkably enhanced mucus barrier	1802:1836	a remarkably enhanced mucus barrier in the EPA group	1802:1853	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	1	91	theme	remitting	265:273	arg1	course					289:294	a continuously remitting and relapsing course	250:294	a continuously remitting and relapsing course	250:294	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	9	92	theme	DSS-induced	1534:1544	arg1	colitis					1546:1552	DSS-induced colitis	1534:1552	DSS-induced colitis	1534:1552	Results: Compared with DHA, the more potent inhibitory effect of high dose EPA on DSS-induced colitis was reconfirmed, which was underlain by a reinforced mucus layer as indicated by increased mucin granule release, mucus layer stratification and markedly upregulated expression of the key modulators involved in goblet cell differentiation.
35297435	3	93	theme	mucus	391:395	arg1	necessary					442:450	necessary	442:450	necessary	442:450	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	3	93	theme	mucus	391:395	arg1	lining					405:410	The mucus barrier lining the gastrointestinal tract	387:437	The mucus barrier lining the gastrointestinal tract	387:437	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	1	94	theme	relapsing	279:287	arg1	course					289:294	a continuously remitting and relapsing course	250:294	a continuously remitting and relapsing course	250:294	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	7	95	theme	Western	1238:1244	arg1	blotting					1246:1253	Western blotting	1238:1253	Western blotting	1238:1253	Western blotting was used to analyze the underlying molecular events.
35297435	10	96	theme	EPA	1845:1847	arg1	group					1849:1853	the EPA group	1841:1853	the EPA group	1841:1853	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	3	97	theme	gut	475:477	arg1	interaction					490:500	host and gut microbiota interaction	466:500	interaction	490:500	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	4	98	theme	respective	765:774	arg1	effects					776:782	their respective effects	759:782	their respective effects	759:782	Differential effects of the anti-inflammatory fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on UC progression in mice were firstly addressed by our previous work; here, the mechanism for their respective effects were further uncovered from host-microbiome crosstalk based on mucus barrier modulation to pave the way for UC therapy.
35297435	5	99	theme	histopathology	958:971	arg1	score					973:977	histopathology score	958:977	histopathology score	958:977	Methods: Assessment of the disease activity index and histopathology score was conducted in mice with dextran sodium sulfate (DSS)-induced colitis pre-treated with different doses of EPA and DHA.
35297435	10	100	theme	propionic	2043:2051	arg1	acids					2053:2057	acetic and propionic acids	2032:2057	acetic and propionic acids	2032:2057	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	3	101	theme	host	466:469	arg1	interaction					490:500	host and gut microbiota interaction	466:500	interaction	490:500	The mucus barrier lining the gastrointestinal tract is necessary to coordinate host and gut microbiota interaction by nourishing and modulating the microbiota.
35297435	10	102	theme	acetic	2032:2037	arg1	acids					2053:2057	acetic and propionic acids	2032:2057	acetic and propionic acids	2032:2057	In turn a remarkably enhanced mucus barrier in the EPA group functioned to modulate the gut microbiome, as demonstrated by the enriched abundance of the phylum Bacteroidetes and mucin-degrading bacterium Akkermansia muciniphila producing acetic and propionic acids.
35297435	8	103	theme	gut	1386:1388	arg1	composition					1400:1410	the gut microbial composition	1382:1410	the gut microbial composition	1382:1410	Fecal short chain fatty acids were detected using gas chromatography, and the gut microbial composition was analyzed using 16S rRNA sequencing.
35297435	2	104	theme	abnormal	332:339	arg1	interactions					341:352	abnormal interactions	332:352	abnormal interactions between host and gut microbiota	332:384	Its etiology is closely related to abnormal interactions between host and gut microbiota.
35297435	1	105	theme	inflammatory	210:221	arg1	colitis					184:190	Ulcerative colitis	173:190	Ulcerative colitis (UC)	173:195	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	105	theme	inflammatory	210:221	arg1	Background					161:170	Background	161:170	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.	161:295	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
35297435	1	105	theme	inflammatory	210:221	arg1	disorder					223:230	a chronic inflammatory disorder	200:230	a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course	200:294	Background: Ulcerative colitis (UC) is a chronic inflammatory disorder of the colon with a continuously remitting and relapsing course.
37279388	9	0	theme	MUC1	1584:1587	arg1	cells					1621:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	6	1	theme	leukocyte	1040:1048	arg1	class					1058:1062	human leukocyte antigen class II or I	1034:1070	class	1058:1062	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	3	2	theme	carbohydrate	472:483	arg1	TACAs					495:499	TACAs	495:499	TACAs	495:499	Dendritic cells (DCs) express lectins that bind to these tumor-associated carbohydrate antigens (TACAs) to modulate immune responses.
37279388	3	2	theme	carbohydrate	472:483	arg1	antigens					485:492	these tumor-associated carbohydrate antigens	449:492	these tumor-associated carbohydrate antigens (TACAs)	449:500	Dendritic cells (DCs) express lectins that bind to these tumor-associated carbohydrate antigens (TACAs) to modulate immune responses.
37279388	9	3	theme	MUC1-positive	1593:1605	arg1	cancer					1614:1619	MUC1-positive breast cancer	1593:1619	MUC1-positive breast cancer	1593:1619	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	9	4	theme	cancer	1614:1619	arg1	cells					1621:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	6	5	dep	it	1080:1081	arg1	target					1097:1102	an attractive target	1083:1102	it an attractive target for anticancer vaccines	1080:1126	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	5	6	theme	tripartite	761:770	arg1	candidate					780:788	a modular tripartite vaccine candidate	751:788	a modular tripartite vaccine candidate	751:788	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	8	7	dep	In	1290:1291	arg1	vivo					1293:1296	vivo	1293:1296	vivo	1293:1296	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	6	8	theme	attractive	1086:1095	arg1	target					1097:1102	an attractive target	1083:1102	it an attractive target for anticancer vaccines	1080:1126	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	8	9	with	immunization	1320:1331	arg1	construct					1365:1373	the newly designed vaccine construct	1338:1373	the newly designed vaccine construct bearing the GalNAc glycocluster	1338:1405	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	2	10	theme	several	299:305	arg1	tumors					313:318	several solid tumors	299:318	several solid tumors	299:318	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	1	11	theme	glycosylation	189:201	arg1	patterns					203:210	Mucin expression and glycosylation patterns	168:210	patterns	203:210	Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells.
37279388	6	12	theme	human	1034:1038	arg1	class					1058:1062	human leukocyte antigen class II or I	1034:1070	class	1058:1062	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	9	13	from	cells	1621:1625	arg1	library					1531:1537	a library	1529:1537	a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells	1529:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	8	14	theme	vaccine	1357:1363	arg1	construct					1365:1373	the newly designed vaccine construct	1338:1373	the newly designed vaccine construct bearing the GalNAc glycocluster	1338:1405	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	5	15	theme	high-affinity	807:819	arg1	glycocluster					821:832	a high-affinity glycocluster	805:832	a high-affinity glycocluster based on a tetraphenylethylene scaffold	805:872	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	0	16	theme	Improved	141:148	arg1	Response					158:165	an Improved Humoral Response	138:165	an Improved Humoral Response	138:165	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	10	17	theme	antibody	1749:1756	arg1	production					1758:1767	antibody production	1749:1767	antibody production	1749:1767	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	1	18	from	patterns	203:210	arg1	cells					222:226	cancer cells	215:226	cancer cells	215:226	Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells.
37279388	8	19	theme	GalNAc	1387:1392	arg1	glycocluster					1394:1405	the GalNAc glycocluster	1383:1405	the GalNAc glycocluster	1383:1405	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	2	20	theme	Mucin	264:268	arg1	MUC1					273:276	Mucin 1 (MUC1)	264:277	Mucin 1 (MUC1)	264:277	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	5	21	theme	galactose-type	900:913	arg1	MGL					923:925	MGL	923:925	MGL	923:925	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	5	21	theme	galactose-type	900:913	arg1	lectin					915:920	the macrophage galactose-type lectin	885:920	the macrophage galactose-type lectin (MGL)	885:926	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	7	22	theme	TACA	1269:1272	arg1	recognition					1250:1260	recognition	1250:1260	recognition	1250:1260	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen is shown to promote uptake and recognition of the TACA by DCs via MGL.
37279388	7	22	theme	TACA	1269:1272	arg1	uptake					1239:1244	uptake	1239:1244	uptake	1239:1244	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen is shown to promote uptake and recognition of the TACA by DCs via MGL.
37279388	4	23	theme	TACA	662:665	arg1	tolerance					667:675	TACA tolerance	662:675	TACA tolerance	662:675	Selectively targeting these receptors with synthetic TACAs is a promising strategy to develop anticancer vaccines and to overcome TACA tolerance.
37279388	5	24	theme	solid	711:715	arg1	approach					741:748	a solid phase peptide synthesis approach	709:748	a solid phase peptide synthesis approach	709:748	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	6	25	theme	C-type	966:971	arg1	receptor					980:987	a C-type lectin receptor	964:987	a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines	964:1126	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	6	25	theme	C-type	966:971	arg1	MGL					957:959	MGL	957:959	MGL	957:959	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	8	26	theme	higher	1417:1422	arg1	titer					1424:1428	a higher titer	1415:1428	a higher titer of anti-Tn-MUC1 antibodies	1415:1455	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	0	27	theme	Macrophage	74:83	arg1	Lectin					102:107	the Macrophage Galactose C-type Lectin	70:107	the Macrophage Galactose C-type Lectin on Dendritic Cells	70:126	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	7	28	theme	glycocluster	1148:1159	arg1	Conjugation					1129:1139	Conjugation	1129:1139	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen	1129:1217	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen is shown to promote uptake and recognition of the TACA by DCs via MGL.
37279388	0	29	theme	C-type	95:100	arg1	Lectin					102:107	the Macrophage Galactose C-type Lectin	70:107	the Macrophage Galactose C-type Lectin on Dendritic Cells	70:126	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	5	30	theme	antigen	931:937	arg1	cells					950:954	antigen presenting cells	931:954	antigen presenting cells	931:954	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	9	31	from	structures	1570:1579	arg1	cells					1621:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	8	32	theme	antibodies	1446:1455	arg1	titer					1424:1428	a higher titer	1415:1428	a higher titer of anti-Tn-MUC1 antibodies	1415:1455	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	2	33	theme	O-glycans	368:376	arg1	levels					338:343	high levels	333:343	high levels of aberrant, truncated O-glycans (e.g., Tn antigen)	333:395	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	0	34	theme	MUC1	0:3	arg1	Vaccine					18:24	MUC1 Glycopeptide Vaccine	0:24	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster	0:60	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	9	35	from	library	1531:1537	arg1	cells					1621:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	MUC1 and MUC1-positive breast cancer cells	1584:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	7	36	gly	glycopeptides	1182:1194	arg2	glycopeptides					1182:1194	MUC1 glycopeptides	1177:1194	MUC1 glycopeptides bearing the Tn antigen	1177:1217	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen is shown to promote uptake and recognition of the TACA by DCs via MGL.
37279388	2	37	theme	Tn	385:386	arg1	antigen					388:394	Tn antigen	385:394	Tn antigen	385:394	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	2	38	theme	high	333:336	arg1	levels					338:343	high levels	333:343	high levels of aberrant, truncated O-glycans (e.g., Tn antigen)	333:395	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	0	39	theme	GalNAc	42:47	arg1	Glycocluster					49:60	a GalNAc Glycocluster	40:60	a GalNAc Glycocluster	40:60	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	10	40	theme	MUC1	1694:1697	arg1	antigens					1712:1719	tumor-associated MUC1 glycopeptide antigens	1677:1719	tumor-associated MUC1 glycopeptide antigens	1677:1719	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	9	41	theme	saccharide	1559:1568	arg1	structures					1570:1579	tumor-associated saccharide structures	1542:1579	tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells	1542:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	7	42	theme	Tn	1208:1209	arg1	antigen					1211:1217	the Tn antigen	1204:1217	the Tn antigen	1204:1217	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen is shown to promote uptake and recognition of the TACA by DCs via MGL.
37279388	4	43	theme	synthetic	575:583	arg1	TACAs					585:589	synthetic TACAs	575:589	synthetic TACAs	575:589	Selectively targeting these receptors with synthetic TACAs is a promising strategy to develop anticancer vaccines and to overcome TACA tolerance.
37279388	8	44	theme	designed	1348:1355	arg1	construct					1365:1373	the newly designed vaccine construct	1338:1373	the newly designed vaccine construct bearing the GalNAc glycocluster	1338:1405	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	10	45	theme	tumor-associated	1677:1692	arg1	antigens					1712:1719	tumor-associated MUC1 glycopeptide antigens	1677:1719	tumor-associated MUC1 glycopeptide antigens	1677:1719	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	6	46	theme	antigen	1050:1056	arg1	class					1058:1062	human leukocyte antigen class II or I	1034:1070	class	1058:1062	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	5	47	theme	phase	717:721	arg1	approach					741:748	a solid phase peptide synthesis approach	709:748	a solid phase peptide synthesis approach	709:748	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	3	48	theme	tumor-associated	455:470	arg1	TACAs					495:499	TACAs	495:499	TACAs	495:499	Dendritic cells (DCs) express lectins that bind to these tumor-associated carbohydrate antigens (TACAs) to modulate immune responses.
37279388	3	48	theme	tumor-associated	455:470	arg1	antigens					485:492	these tumor-associated carbohydrate antigens	449:492	these tumor-associated carbohydrate antigens (TACAs)	449:500	Dendritic cells (DCs) express lectins that bind to these tumor-associated carbohydrate antigens (TACAs) to modulate immune responses.
37279388	7	49	theme	MUC1	1177:1180	arg1	glycopeptides					1182:1194	MUC1 glycopeptides	1177:1194	MUC1 glycopeptides bearing the Tn antigen	1177:1217	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen is shown to promote uptake and recognition of the TACA by DCs via MGL.
37279388	9	50	theme	breast	1607:1612	arg1	cancer					1614:1619	MUC1-positive breast cancer	1593:1619	MUC1-positive breast cancer	1593:1619	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	6	51	theme	anticancer	1108:1117	arg1	vaccines					1119:1126	anticancer vaccines	1108:1126	anticancer vaccines	1108:1126	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	10	52	theme	ligand	1659:1664	arg1	Conjugation					1628:1638	Conjugation	1628:1638	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens	1628:1719	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	10	53	theme	glycopeptide	1699:1710	arg1	antigens					1712:1719	tumor-associated MUC1 glycopeptide antigens	1677:1719	tumor-associated MUC1 glycopeptide antigens	1677:1719	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	1	54	theme	Mucin	168:172	arg1	expression					174:183	Mucin expression and glycosylation patterns	168:210	expression	174:183	Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells.
37279388	4	55	theme	anticancer	626:635	arg1	vaccines					637:644	anticancer vaccines	626:644	anticancer vaccines	626:644	Selectively targeting these receptors with synthetic TACAs is a promising strategy to develop anticancer vaccines and to overcome TACA tolerance.
37279388	5	56	theme	peptide	723:729	arg1	approach					741:748	a solid phase peptide synthesis approach	709:748	a solid phase peptide synthesis approach	709:748	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	2	57	theme	solid	307:311	arg1	tumors					313:318	several solid tumors	299:318	several solid tumors	299:318	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	3	58	theme	immune	514:519	arg1	responses					521:529	immune responses	514:529	immune responses	514:529	Dendritic cells (DCs) express lectins that bind to these tumor-associated carbohydrate antigens (TACAs) to modulate immune responses.
37279388	4	59	theme	promising	596:604	arg1	strategy					606:613	a promising strategy	594:613	a promising strategy to develop anticancer vaccines and to overcome TACA tolerance	594:675	Selectively targeting these receptors with synthetic TACAs is a promising strategy to develop anticancer vaccines and to overcome TACA tolerance.
37279388	1	60	theme	cancer	215:220	arg1	cells					222:226	cancer cells	215:226	cancer cells	215:226	Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells.
37279388	0	61	theme	Humoral	150:156	arg1	Response					158:165	an Improved Humoral Response	138:165	an Improved Humoral Response	138:165	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	5	62	theme	macrophage	889:898	arg1	MGL					923:925	MGL	923:925	MGL	923:925	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	5	62	theme	macrophage	889:898	arg1	lectin					915:920	the macrophage galactose-type lectin	885:920	the macrophage galactose-type lectin (MGL)	885:926	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	10	63	theme	synergistic	1727:1737	arg1	impact					1739:1744	a synergistic impact	1725:1744	a synergistic impact on antibody production	1725:1767	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	0	64	from	Lectin	102:107	arg1	Cells					122:126	Dendritic Cells	112:126	Dendritic Cells	112:126	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	5	65	theme	tetraphenylethylene	845:863	arg1	scaffold					865:872	a tetraphenylethylene scaffold	843:872	a tetraphenylethylene scaffold	843:872	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	6	66	theme	lectin	973:978	arg1	receptor					980:987	a C-type lectin receptor	964:987	a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines	964:1126	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	6	66	theme	lectin	973:978	arg1	MGL					957:959	MGL	957:959	MGL	957:959	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	0	67	theme	Galactose	85:93	arg1	Lectin					102:107	the Macrophage Galactose C-type Lectin	70:107	the Macrophage Galactose C-type Lectin on Dendritic Cells	70:126	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	1	68	from	expression	174:183	arg1	cells					222:226	cancer cells	215:226	cancer cells	215:226	Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells.
37279388	10	69	contain	has	1721:1723	arg2	impact					1739:1744	a synergistic impact	1725:1744	a synergistic impact on antibody production	1725:1767	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	10	69	contain	has	1721:1723	arg1	Conjugation					1628:1638	Conjugation	1628:1638	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens	1628:1719	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	8	70	theme	anti-Tn-MUC1	1433:1444	arg1	antibodies					1446:1455	anti-Tn-MUC1 antibodies	1433:1455	anti-Tn-MUC1 antibodies	1433:1455	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	10	71	from	impact	1739:1744	arg1	production					1758:1767	antibody production	1749:1767	antibody production	1749:1767	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	0	72	theme	Dendritic	112:120	arg1	Cells					122:126	Dendritic Cells	112:126	Dendritic Cells	112:126	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	5	73	theme	presenting	939:948	arg1	cells					950:954	antigen presenting cells	931:954	antigen presenting cells	931:954	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	2	74	dep	aberrant	348:355	arg1	truncated					358:366	truncated	358:366	truncated	358:366	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	5	75	theme	modular	753:759	arg1	candidate					780:788	a modular tripartite vaccine candidate	751:788	a modular tripartite vaccine candidate	751:788	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	3	76	theme	Dendritic	398:406	arg1	cells					408:412	Dendritic cells	398:412	Dendritic cells (DCs)	398:418	Dendritic cells (DCs) express lectins that bind to these tumor-associated carbohydrate antigens (TACAs) to modulate immune responses.
37279388	3	76	theme	Dendritic	398:406	arg1	DCs					415:417	DCs	415:417	DCs	415:417	Dendritic cells (DCs) express lectins that bind to these tumor-associated carbohydrate antigens (TACAs) to modulate immune responses.
37279388	5	77	theme	vaccine	772:778	arg1	candidate					780:788	a modular tripartite vaccine candidate	751:788	a modular tripartite vaccine candidate	751:788	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	8	78	theme	In	1290:1291	arg1	testing					1298:1304	In vivo testing	1290:1304	In vivo testing	1290:1304	In vivo testing revealed that immunization with the newly designed vaccine construct bearing the GalNAc glycocluster induced a higher titer of anti-Tn-MUC1 antibodies compared to the TACAs alone.
37279388	5	79	theme	synthesis	731:739	arg1	approach					741:748	a solid phase peptide synthesis approach	709:748	a solid phase peptide synthesis approach	709:748	In this work, we prepared, via a solid phase peptide synthesis approach, a modular tripartite vaccine candidate, incorporating a high-affinity glycocluster based on a tetraphenylethylene scaffold, to target the macrophage galactose-type lectin (MGL) on antigen presenting cells.
37279388	6	80	theme	Tn	1000:1001	arg1	antigens					1003:1010	Tn antigens	1000:1010	Tn antigens	1000:1010	MGL is a C-type lectin receptor that binds Tn antigens and can route them to human leukocyte antigen class II or I, making it an attractive target for anticancer vaccines.
37279388	4	81	dep	strategy	606:613	arg1	overcome					653:660	overcome	653:660	to overcome TACA tolerance	650:675	Selectively targeting these receptors with synthetic TACAs is a promising strategy to develop anticancer vaccines and to overcome TACA tolerance.
37279388	4	81	dep	strategy	606:613	arg1	develop					618:624	develop	618:624	to develop anticancer vaccines	615:644	Selectively targeting these receptors with synthetic TACAs is a promising strategy to develop anticancer vaccines and to overcome TACA tolerance.
37279388	2	82	dep	antigen	388:394	arg1	e.g.					379:382	e.g.	379:382	e.g.	379:382	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	10	83	theme	high-affinity	1645:1657	arg1	ligand					1659:1664	a high-affinity ligand	1643:1664	a high-affinity ligand for MGL	1643:1672	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	10	84	gly	glycopeptide	1699:1710	arg2	glycopeptide					1699:1710	tumor-associated MUC1 glycopeptide antigens	1677:1719	tumor-associated MUC1 glycopeptide antigens	1677:1719	Conjugation of a high-affinity ligand for MGL to tumor-associated MUC1 glycopeptide antigens has a synergistic impact on antibody production.
37279388	9	85	theme	tumor-associated	1542:1557	arg1	structures					1570:1579	tumor-associated saccharide structures	1542:1579	tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells	1542:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	2	86	theme	aberrant	348:355	arg1	O-glycans					368:376	aberrant, truncated O-glycans	348:376	aberrant, truncated O-glycans (e.g., Tn antigen)	348:395	Mucin 1 (MUC1) is overexpressed in several solid tumors and presents high levels of aberrant, truncated O-glycans (e.g., Tn antigen).
37279388	9	87	theme	structures	1570:1579	arg1	library					1531:1537	a library	1529:1537	a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells	1529:1625	Additionally, the antibodies obtained bind a library of tumor-associated saccharide structures on MUC1 and MUC1-positive breast cancer cells.
37279388	7	88	theme	glycopeptides	1182:1194	arg1	library					1166:1172	a library	1164:1172	a library of MUC1 glycopeptides bearing the Tn antigen	1164:1217	Conjugation of the glycocluster to a library of MUC1 glycopeptides bearing the Tn antigen is shown to promote uptake and recognition of the TACA by DCs via MGL.
37279388	0	89	theme	Glycopeptide	5:16	arg1	Vaccine					18:24	MUC1 Glycopeptide Vaccine	0:24	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster	0:60	MUC1 Glycopeptide Vaccine Modified with a GalNAc Glycocluster Targets the Macrophage Galactose C-type Lectin on Dendritic Cells to Elicit an Improved Humoral Response.
37279388	1	90	theme	healthy	249:255	arg1	cells					257:261	healthy cells	249:261	healthy cells	249:261	Mucin expression and glycosylation patterns on cancer cells differ markedly from healthy cells.
36613370	0	0	from	Polysaccharides	15:29	arg1	Bread					61:65	Hull-Less Barley Sourdough Bread	34:65	Hull-Less Barley Sourdough Bread	34:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	4	1	theme	β-glucans	856:864	arg1	content					845:851	a higher content	836:851	a higher content of β-glucans	836:864	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	1	theme	β-glucans	856:864	arg1	content					878:884	a lower content	870:884	a lower content of starch	870:894	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	2	theme	β-glucans	1108:1116	arg1	amount					1030:1035	a higher amount	1021:1035	a higher amount of starch	1021:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	2	theme	β-glucans	1108:1116	arg1	fructans					1063:1070	fructans	1063:1070	fructans	1063:1070	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	2	theme	β-glucans	1108:1116	arg1	content					1097:1103	a lower content	1089:1103	a lower content of β-glucans	1089:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	2	theme	β-glucans	1108:1116	arg1	mannans					1076:1082	mannans	1076:1082	mannans	1076:1082	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	2	theme	β-glucans	1108:1116	arg1	starch					1040:1045	starch	1040:1045	starch	1040:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	2	theme	β-glucans	1108:1116	arg1	β-glucans					1108:1116	β-glucans	1108:1116	β-glucans	1108:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	2	theme	β-glucans	1108:1116	arg1	glucans					1054:1060	total glucans	1048:1060	total glucans	1048:1060	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	5	3	theme	method	1205:1210	arg1	type					1174:1177	the type	1170:1177	the type of flour and fermentation method used	1170:1215	The composition of polysaccharides was affected by the type of flour and fermentation method used.
36613370	2	4	theme	fructans	335:342	arg1	changes					303:309	the changes	299:309	the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast	299:493	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	0	5	from	Composition	0:10	arg1	Properties					96:105	Physical Properties	87:105	Physical Properties of Bread	87:114	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	0	5	from	Composition	0:10	arg1	Bread					61:65	Hull-Less Barley Sourdough Bread	34:65	Hull-Less Barley Sourdough Bread	34:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	2	6	from	changes	303:309	arg1	breads					385:390	wholegrain wheat breads	368:390	wholegrain wheat breads	368:390	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	2	6	from	changes	303:309	arg1	barley					357:362	hull-less barley	347:362	hull-less barley	347:362	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	0	7	theme	Physical	87:94	arg1	Properties					96:105	Physical Properties	87:105	Physical Properties of Bread	87:114	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	0	8	from	Bread	61:65	arg1	Impact					77:82	Their Impact	71:82	Their Impact on Physical Properties of Bread	71:114	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	0	8	from	Bread	61:65	arg1	Composition					0:10	Composition	0:10	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread	0:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	2	9	theme	bread	578:582	arg1	parameters					564:573	the physical parameters	551:573	the physical parameters of bread	551:582	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	0	10	theme	Bread	110:114	arg1	Properties					96:105	Physical Properties	87:105	Physical Properties of Bread	87:114	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	2	11	theme	research	270:277	arg1	aim					259:261	The aim	255:261	The aim of the research	255:277	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	2	12	theme	hull-less	458:466	arg1	sourdough					475:483	germinated hull-less barley sourdough	447:483	germinated hull-less barley sourdough	447:483	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	4	13	theme	wholegrain	966:975	arg1	breads					983:988	wholegrain wheat breads	966:988	wholegrain wheat breads	966:988	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	14	theme	lower	1091:1095	arg1	content					1097:1103	a lower content	1089:1103	a lower content of β-glucans	1089:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	2	15	theme	wheat	379:383	arg1	breads					385:390	wholegrain wheat breads	368:390	wholegrain wheat breads	368:390	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	2	16	theme	germinated	447:456	arg1	sourdough					475:483	germinated hull-less barley sourdough	447:483	germinated hull-less barley sourdough	447:483	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	4	17	contain	have	1016:1019	arg1	breads					983:988	wholegrain wheat breads	966:988	wholegrain wheat breads	966:988	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	17	contain	have	1016:1019	arg1	glucans					903:909	total glucans	897:909	total glucans	897:909	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	17	contain	have	1016:1019	arg2	starch					1040:1045	starch	1040:1045	starch	1040:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	17	contain	have	1016:1019	arg2	glucans					1054:1060	total glucans	1048:1060	total glucans	1048:1060	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	17	contain	have	1016:1019	arg2	content					1097:1103	a lower content	1089:1103	a lower content of β-glucans	1089:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	17	contain	have	1016:1019	arg2	β-glucans					1108:1116	β-glucans	1108:1116	β-glucans	1108:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	17	contain	have	1016:1019	arg1	fructans					912:919	fructans	912:919	fructans	912:919	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	17	contain	have	1016:1019	arg2	amount					1030:1035	a higher amount	1021:1035	a higher amount of starch	1021:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	2	18	theme	wholegrain	368:377	arg1	breads					385:390	wholegrain wheat breads	368:390	wholegrain wheat breads	368:390	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	4	19	theme	higher	838:843	arg1	content					845:851	a higher content	836:851	a higher content of β-glucans	836:864	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	3	20	theme	dough	643:647	arg1	fermentation					649:660	wholegrain wheat bread dough fermentation	620:660	wholegrain wheat bread dough fermentation	620:660	By using the barley sourdoughs for wholegrain wheat bread dough fermentation, the specific volume and porosity was reduced; the hardness was not significantly increased, but the content of β-glucans was doubled.
36613370	4	21	theme	total	897:901	arg1	glucans					903:909	total glucans	897:909	total glucans	897:909	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	2	22	theme	hull-less	419:427	arg1	sourdough					436:444	spontaneous hull-less barley sourdough	407:444	spontaneous hull-less barley sourdough	407:444	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	2	23	theme	barley	429:434	arg1	sourdough					436:444	spontaneous hull-less barley sourdough	407:444	spontaneous hull-less barley sourdough	407:444	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	2	24	theme	polysaccharides	532:546	arg1	impact					522:527	the impact	518:527	the impact of polysaccharides on the physical parameters of bread	518:582	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	3	25	theme	bread	637:641	arg1	dough					643:647	wholegrain wheat bread dough	620:647	wholegrain wheat bread dough fermentation	620:660	By using the barley sourdoughs for wholegrain wheat bread dough fermentation, the specific volume and porosity was reduced; the hardness was not significantly increased, but the content of β-glucans was doubled.
36613370	1	26	theme	grain	155:159	arg1	polysaccharides					132:146	polysaccharides	132:146	polysaccharides of the grain	132:159	The complex of polysaccharides of the grain transforms during processing and modifies the physical and chemical characteristics of bread.
36613370	2	27	theme	spontaneous	407:417	arg1	sourdough					436:444	spontaneous hull-less barley sourdough	407:444	spontaneous hull-less barley sourdough	407:444	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	0	28	theme	Barley	44:49	arg1	Bread					61:65	Hull-Less Barley Sourdough Bread	34:65	Hull-Less Barley Sourdough Bread	34:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	5	29	theme	polysaccharides	1138:1152	arg1	composition					1123:1133	The composition	1119:1133	The composition of polysaccharides	1119:1152	The composition of polysaccharides was affected by the type of flour and fermentation method used.
36613370	0	30	from	Impact	77:82	arg1	Properties					96:105	Physical Properties	87:105	Physical Properties of Bread	87:114	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	0	30	from	Impact	77:82	arg1	Bread					61:65	Hull-Less Barley Sourdough Bread	34:65	Hull-Less Barley Sourdough Bread	34:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	4	31	theme	Principal	797:805	arg1	analysis					817:824	Principal component analysis	797:824	Principal component analysis	797:824	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	1	32	theme	physical	207:214	arg1	characteristics					229:243	the physical and chemical characteristics	203:243	the physical and chemical characteristics of bread	203:252	The complex of polysaccharides of the grain transforms during processing and modifies the physical and chemical characteristics of bread.
36613370	0	33	theme	Hull-Less	34:42	arg1	Bread					61:65	Hull-Less Barley Sourdough Bread	34:65	Hull-Less Barley Sourdough Bread	34:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	4	34	theme	wheat	977:981	arg1	breads					983:988	wholegrain wheat breads	966:988	wholegrain wheat breads	966:988	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	3	35	theme	wholegrain	620:629	arg1	dough					643:647	wholegrain wheat bread dough	620:647	wholegrain wheat bread dough fermentation	620:660	By using the barley sourdoughs for wholegrain wheat bread dough fermentation, the specific volume and porosity was reduced; the hardness was not significantly increased, but the content of β-glucans was doubled.
36613370	3	36	theme	β-glucans	774:782	arg1	content					763:769	the content	759:769	the content of β-glucans	759:782	By using the barley sourdoughs for wholegrain wheat bread dough fermentation, the specific volume and porosity was reduced; the hardness was not significantly increased, but the content of β-glucans was doubled.
36613370	2	37	theme	barley	468:473	arg1	sourdough					475:483	germinated hull-less barley sourdough	447:483	germinated hull-less barley sourdough	447:483	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	3	38	theme	wheat	631:635	arg1	dough					643:647	wholegrain wheat bread dough	620:647	wholegrain wheat bread dough fermentation	620:660	By using the barley sourdoughs for wholegrain wheat bread dough fermentation, the specific volume and porosity was reduced; the hardness was not significantly increased, but the content of β-glucans was doubled.
36613370	4	39	theme	starch	889:894	arg1	content					845:851	a higher content	836:851	a higher content of β-glucans	836:864	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	39	theme	starch	889:894	arg1	content					878:884	a lower content	870:884	a lower content of starch	870:894	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	0	40	theme	Sourdough	51:59	arg1	Bread					61:65	Hull-Less Barley Sourdough Bread	34:65	Hull-Less Barley Sourdough Bread	34:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	4	41	theme	lower	872:876	arg1	content					878:884	a lower content	870:884	a lower content of starch	870:894	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	42	theme	component	807:815	arg1	analysis					817:824	Principal component analysis	797:824	Principal component analysis	797:824	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	2	43	theme	mannans	323:329	arg1	changes					303:309	the changes	299:309	the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast	299:493	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	3	44	theme	barley	598:603	arg1	sourdoughs					605:614	the barley sourdoughs	594:614	the barley sourdoughs for wholegrain wheat bread dough fermentation	594:660	By using the barley sourdoughs for wholegrain wheat bread dough fermentation, the specific volume and porosity was reduced; the hardness was not significantly increased, but the content of β-glucans was doubled.
36613370	2	45	theme	physical	555:562	arg1	parameters					564:573	the physical parameters	551:573	the physical parameters of bread	551:582	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	3	46	theme	specific	667:674	arg1	volume					676:681	the specific volume	663:681	the specific volume	663:681	By using the barley sourdoughs for wholegrain wheat bread dough fermentation, the specific volume and porosity was reduced; the hardness was not significantly increased, but the content of β-glucans was doubled.
36613370	4	47	theme	starch	1040:1045	arg1	amount					1030:1035	a higher amount	1021:1035	a higher amount of starch	1021:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	47	theme	starch	1040:1045	arg1	fructans					1063:1070	fructans	1063:1070	fructans	1063:1070	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	47	theme	starch	1040:1045	arg1	content					1097:1103	a lower content	1089:1103	a lower content of β-glucans	1089:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	47	theme	starch	1040:1045	arg1	mannans					1076:1082	mannans	1076:1082	mannans	1076:1082	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	47	theme	starch	1040:1045	arg1	starch					1040:1045	starch	1040:1045	starch	1040:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	47	theme	starch	1040:1045	arg1	β-glucans					1108:1116	β-glucans	1108:1116	β-glucans	1108:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	47	theme	starch	1040:1045	arg1	glucans					1054:1060	total glucans	1048:1060	total glucans	1048:1060	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	1	48	theme	chemical	220:227	arg1	characteristics					229:243	the physical and chemical characteristics	203:243	the physical and chemical characteristics of bread	203:252	The complex of polysaccharides of the grain transforms during processing and modifies the physical and chemical characteristics of bread.
36613370	2	49	theme	glucans	314:320	arg1	changes					303:309	the changes	299:309	the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast	299:493	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	2	50	from	impact	522:527	arg1	parameters					564:573	the physical parameters	551:573	the physical parameters of bread	551:582	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	4	51	theme	barley	947:952	arg1	breads					954:959	hull-less barley breads	937:959	hull-less barley breads	937:959	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	5	52	theme	fermentation	1192:1203	arg1	method					1205:1210	fermentation method	1192:1210	fermentation method	1192:1210	The composition of polysaccharides was affected by the type of flour and fermentation method used.
36613370	1	53	theme	polysaccharides	132:146	arg1	complex					121:127	The complex	117:127	The complex of polysaccharides of the grain	117:159	The complex of polysaccharides of the grain transforms during processing and modifies the physical and chemical characteristics of bread.
36613370	4	54	theme	total	1048:1052	arg1	glucans					1054:1060	total glucans	1048:1060	total glucans	1048:1060	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	5	55	theme	flour	1182:1186	arg1	type					1174:1177	the type	1170:1177	the type of flour and fermentation method used	1170:1215	The composition of polysaccharides was affected by the type of flour and fermentation method used.
36613370	4	56	theme	hull-less	937:945	arg1	breads					954:959	hull-less barley breads	937:959	hull-less barley breads	937:959	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	0	57	theme	Polysaccharides	15:29	arg1	Impact					77:82	Their Impact	71:82	Their Impact on Physical Properties of Bread	71:114	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	0	57	theme	Polysaccharides	15:29	arg1	Composition					0:10	Composition	0:10	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread	0:65	Composition of Polysaccharides in Hull-Less Barley Sourdough Bread and Their Impact on Physical Properties of Bread.
36613370	2	58	theme	hull-less	347:355	arg1	barley					357:362	hull-less barley	347:362	hull-less barley	347:362	The aim of the research was to characterize the changes of glucans, mannans and fructans in hull-less barley and wholegrain wheat breads fermented with spontaneous hull-less barley sourdough, germinated hull-less barley sourdough and yeast, as well as to analyze the impact of polysaccharides on the physical parameters of bread.
36613370	4	59	theme	higher	1023:1028	arg1	amount					1030:1035	a higher amount	1021:1035	a higher amount of starch	1021:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	59	theme	higher	1023:1028	arg1	β-glucans					1108:1116	β-glucans	1108:1116	β-glucans	1108:1116	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	4	59	theme	higher	1023:1028	arg1	starch					1040:1045	starch	1040:1045	starch	1040:1045	Principal component analysis indicates a higher content of β-glucans and a lower content of starch, total glucans, fructans and mannans for hull-less barley breads, but wholegrain wheat breads fermented with sourdoughs have a higher amount of starch, total glucans, fructans and mannans, and a lower content of β-glucans.
36613370	1	60	theme	bread	248:252	arg1	characteristics					229:243	the physical and chemical characteristics	203:243	the physical and chemical characteristics of bread	203:252	The complex of polysaccharides of the grain transforms during processing and modifies the physical and chemical characteristics of bread.
36615338	0	0	from	Mechanism	13:21	arg1	Treatment					43:51	the Treatment	39:51	the Treatment of Experimental Colitis	39:75	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	11	1	theme	phosphorylated	1292:1305	arg1	B					1322:1322	phosphorylated protein kinase B	1292:1322	phosphorylated protein kinase B (AKT)	1292:1328	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	11	1	theme	phosphorylated	1292:1305	arg1	AKT					1325:1327	AKT	1325:1327	AKT	1325:1327	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	1	2	attach	present	145:151	arg1	fruits					171:176	fruits	171:176	fruits	171:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	2	attach	present	145:151	arg2	Quercetin					114:122	Quercetin	114:122	Quercetin	114:122	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	2	attach	present	145:151	arg1	vegetables					156:165	vegetables	156:165	vegetables	156:165	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	2	attach	present	145:151	arg2	present					145:151	present	145:151	present	145:151	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	2	attach	present	145:151	arg2	flavonoid					127:135	a flavonoid	125:135	a flavonoid that is present in vegetables and fruits	125:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	11	3	theme	3-kinase	1355:1362	arg1	expression					1278:1287	the expression	1274:1287	the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K)	1274:1369	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	10	4	theme	inflammatory	1202:1213	arg1	processes					1230:1238	inflammatory and neoplastic processes	1202:1238	inflammatory and neoplastic processes	1202:1238	Network pharmacology revealed that quercetin target genes were enriched in inflammatory and neoplastic processes.
36615338	0	5	theme	Colitis	69:75	arg1	Treatment					43:51	the Treatment	39:51	the Treatment of Experimental Colitis	39:75	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	4	6	theme	%	461:461	arg1	solution					473:480	a 4% (w/v) DSS solution	458:480	a 4% (w/v) DSS solution to drink	458:489	Mice were given a 4% (w/v) DSS solution to drink for 7 days, followed by regular water for the following 5 days.
36615338	5	7	theme	Pharmacological	555:569	arg1	mechanisms					571:580	Pharmacological mechanisms	555:580	Pharmacological mechanisms	555:580	Pharmacological mechanisms were predicted by network pharmacology.
36615338	1	8	from	present	145:151	arg1	fruits					171:176	fruits	171:176	fruits	171:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	8	from	present	145:151	arg1	vegetables					156:165	vegetables	156:165	vegetables	156:165	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	12	9	theme	microbiomes	1531:1541	arg1	re-establishment					1503:1518	re-establishment	1503:1518	re-establishment of healthy microbiomes that favor mucosal healing	1503:1568	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	9	theme	microbiomes	1531:1541	arg1	inhibition					1579:1588	the inhibition	1575:1588	the inhibition of PI3K/AKT signaling	1575:1610	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	9	theme	microbiomes	1531:1541	arg1	regulation					1462:1471	regulation	1462:1471	regulation of the intestinal microbiota	1462:1500	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	0	10	theme	Pharmacology	91:102	arg1	Analysis					104:111	Network Pharmacology Analysis	83:111	Network Pharmacology Analysis	83:111	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	0	11	from	Efficacy	0:7	arg1	Treatment					43:51	the Treatment	39:51	the Treatment of Experimental Colitis	39:75	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	7	12	from	role	835:838	arg1	colon					860:864	the colon	856:864	the colon	856:864	Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon.
36615338	8	13	theme	colon	918:922	arg1	shortening					931:940	colon length shortening	918:940	colon length shortening	918:940	Quercetin attenuated DSS-induced body weight loss, colon length shortening, and pathological damage to the colon.
36615338	12	14	theme	mucosal	1554:1560	arg1	healing					1562:1568	mucosal healing	1554:1568	mucosal healing	1554:1568	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	0	15	theme	Network	83:89	arg1	Analysis					104:111	Network Pharmacology Analysis	83:111	Network Pharmacology Analysis	83:111	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	8	16	theme	pathological	947:958	arg1	damage					960:965	pathological damage	947:965	pathological damage to the colon	947:978	Quercetin attenuated DSS-induced body weight loss, colon length shortening, and pathological damage to the colon.
36615338	9	17	theme	microbiota	1050:1059	arg1	composition					1020:1030	the composition	1016:1030	the composition of the intestinal microbiota in DSS-induced mice	1016:1079	Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria.
36615338	6	18	theme	rDNA	642:645	arg1	sequencing					647:656	High-throughput 16S rDNA sequencing	622:656	High-throughput 16S rDNA sequencing	622:656	High-throughput 16S rDNA sequencing was performed to detect changes in the intestinal microbiota composition.
36615338	9	19	theme	intestinal	1039:1048	arg1	microbiota					1050:1059	the intestinal microbiota	1035:1059	the intestinal microbiota	1035:1059	Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria.
36615338	12	20	theme	UC	1413:1414	arg1	treatment					1400:1408	the treatment	1396:1408	the treatment of UC	1396:1414	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	3	21	from	mechanism	354:362	arg1	colitis					428:434	ulcerative colitis	417:434	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC)	380:439	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	3	21	from	mechanism	354:362	arg1	UC					437:438	UC	437:438	UC	437:438	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	6	22	theme	16S	638:640	arg1	sequencing					647:656	High-throughput 16S rDNA sequencing	622:656	High-throughput 16S rDNA sequencing	622:656	High-throughput 16S rDNA sequencing was performed to detect changes in the intestinal microbiota composition.
36615338	10	23	theme	target	1172:1177	arg1	genes					1179:1183	quercetin target genes	1162:1183	quercetin target genes	1162:1183	Network pharmacology revealed that quercetin target genes were enriched in inflammatory and neoplastic processes.
36615338	12	24	theme	pharmacological	1422:1436	arg1	mechanisms					1438:1447	pharmacological mechanisms	1422:1447	pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling	1422:1610	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	9	25	from	composition	1020:1030	arg1	mice					1076:1079	DSS-induced mice	1064:1079	DSS-induced mice	1064:1079	Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria.
36615338	6	26	theme	High-throughput	622:636	arg1	sequencing					647:656	High-throughput 16S rDNA sequencing	622:656	High-throughput 16S rDNA sequencing	622:656	High-throughput 16S rDNA sequencing was performed to detect changes in the intestinal microbiota composition.
36615338	3	27	theme	-induced	408:415	arg1	colitis					428:434	ulcerative colitis	417:434	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC)	380:439	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	3	27	theme	-induced	408:415	arg1	UC					437:438	UC	437:438	UC	437:438	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	11	28	theme	phosphatidylinositol	1334:1353	arg1	PI3K					1365:1368	PI3K	1365:1368	PI3K	1365:1368	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	11	28	theme	phosphatidylinositol	1334:1353	arg1	3-kinase					1355:1362	phosphatidylinositol 3-kinase	1334:1362	phosphatidylinositol 3-kinase (PI3K)	1334:1369	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	1	29	located	present	145:151	arg1	fruits					171:176	fruits	171:176	fruits	171:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	29	located	present	145:151	arg2	Quercetin					114:122	Quercetin	114:122	Quercetin	114:122	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	29	located	present	145:151	arg1	vegetables					156:165	vegetables	156:165	vegetables	156:165	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	29	located	present	145:151	arg2	present					145:151	present	145:151	present	145:151	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	29	located	present	145:151	arg2	flavonoid					127:135	a flavonoid	125:135	a flavonoid that is present in vegetables and fruits	125:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	11	30	theme	kinase	1315:1320	arg1	B					1322:1322	phosphorylated protein kinase B	1292:1322	phosphorylated protein kinase B (AKT)	1292:1328	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	11	30	theme	kinase	1315:1320	arg1	AKT					1325:1327	AKT	1325:1327	AKT	1325:1327	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	12	31	theme	signaling	1602:1610	arg1	re-establishment					1503:1518	re-establishment	1503:1518	re-establishment of healthy microbiomes that favor mucosal healing	1503:1568	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	31	theme	signaling	1602:1610	arg1	inhibition					1579:1588	the inhibition	1575:1588	the inhibition of PI3K/AKT signaling	1575:1610	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	31	theme	signaling	1602:1610	arg1	regulation					1462:1471	regulation	1462:1471	regulation of the intestinal microbiota	1462:1500	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	9	32	theme	DSS-induced	1064:1074	arg1	mice					1076:1079	DSS-induced mice	1064:1079	DSS-induced mice	1064:1079	Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria.
36615338	3	33	theme	quercetin	367:375	arg1	effect					327:332	effect	327:332	effect	327:332	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	3	33	theme	quercetin	367:375	arg1	mechanism					354:362	pharmacological mechanism	338:362	pharmacological mechanism	338:362	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	11	34	theme	B	1322:1322	arg1	expression					1278:1287	the expression	1274:1287	the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K)	1274:1369	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	6	35	theme	microbiota	708:717	arg1	composition					719:729	the intestinal microbiota composition	693:729	the intestinal microbiota composition	693:729	High-throughput 16S rDNA sequencing was performed to detect changes in the intestinal microbiota composition.
36615338	11	36	theme	protein	1307:1313	arg1	B					1322:1322	phosphorylated protein kinase B	1292:1322	phosphorylated protein kinase B (AKT)	1292:1328	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	11	36	theme	protein	1307:1313	arg1	AKT					1325:1327	AKT	1325:1327	AKT	1325:1327	Quercetin dramatically inhibited the expression of phosphorylated protein kinase B (AKT) and phosphatidylinositol 3-kinase (PI3K).
36615338	3	37	theme	pharmacological	338:352	arg1	mechanism					354:362	pharmacological mechanism	338:362	pharmacological mechanism	338:362	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	6	38	theme	intestinal	697:706	arg1	composition					719:729	the intestinal microbiota composition	693:729	the intestinal microbiota composition	693:729	High-throughput 16S rDNA sequencing was performed to detect changes in the intestinal microbiota composition.
36615338	4	39	theme	following	537:545	arg1	days					549:552	the following 5 days	533:552	the following 5 days	533:552	Mice were given a 4% (w/v) DSS solution to drink for 7 days, followed by regular water for the following 5 days.
36615338	7	40	theme	Enzyme-linked	732:744	arg1	assay					760:764	Enzyme-linked immunosorbent assay	732:764	Enzyme-linked immunosorbent assay	732:764	Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon.
36615338	9	41	theme	harmful	1109:1115	arg1	bacteria					1117:1124	harmful bacteria	1109:1124	harmful bacteria	1109:1124	Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria.
36615338	7	42	theme	western	770:776	arg1	blotting					778:785	western blotting	770:785	western blotting	770:785	Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon.
36615338	7	43	theme	anti-inflammatory	817:833	arg1	role					835:838	the anti-inflammatory role	813:838	the anti-inflammatory role of quercetin in the colon	813:864	Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon.
36615338	3	44	theme	ulcerative	417:426	arg1	colitis					428:434	ulcerative colitis	417:434	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC)	380:439	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	3	44	theme	ulcerative	417:426	arg1	UC					437:438	UC	437:438	UC	437:438	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	0	45	theme	Quercetin	26:34	arg1	Mechanism					13:21	Mechanism	13:21	Mechanism	13:21	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	0	45	theme	Quercetin	26:34	arg1	Efficacy					0:7	Efficacy	0:7	Efficacy	0:7	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	4	46	dep	%	461:461	arg1	w/v					464:466	w/v	464:466	w/v	464:466	Mice were given a 4% (w/v) DSS solution to drink for 7 days, followed by regular water for the following 5 days.
36615338	3	47	from	effect	327:332	arg1	colitis					428:434	ulcerative colitis	417:434	dextran sodium sulfate (DSS)-induced ulcerative colitis (UC)	380:439	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	3	47	from	effect	327:332	arg1	UC					437:438	UC	437:438	UC	437:438	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	8	48	theme	DSS-induced	888:898	arg1	loss					912:915	DSS-induced body weight loss	888:915	DSS-induced body weight loss	888:915	Quercetin attenuated DSS-induced body weight loss, colon length shortening, and pathological damage to the colon.
36615338	10	49	theme	Network	1127:1133	arg1	pharmacology					1135:1146	Network pharmacology	1127:1146	Network pharmacology	1127:1146	Network pharmacology revealed that quercetin target genes were enriched in inflammatory and neoplastic processes.
36615338	7	50	theme	immunosorbent	746:758	arg1	assay					760:764	Enzyme-linked immunosorbent assay	732:764	Enzyme-linked immunosorbent assay	732:764	Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon.
36615338	12	51	contain	has	1382:1384	arg1	Quercetin					1372:1380	Quercetin	1372:1380	Quercetin	1372:1380	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	51	contain	has	1382:1384	arg2	role					1388:1391	a role	1386:1391	a role	1386:1391	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	5	52	theme	network	600:606	arg1	pharmacology					608:619	network pharmacology	600:619	network pharmacology	600:619	Pharmacological mechanisms were predicted by network pharmacology.
36615338	6	53	from	changes	682:688	arg1	composition					719:729	the intestinal microbiota composition	693:729	the intestinal microbiota composition	693:729	High-throughput 16S rDNA sequencing was performed to detect changes in the intestinal microbiota composition.
36615338	4	54	theme	4	460:460	arg1	%					461:461	%	461:461	%	461:461	Mice were given a 4% (w/v) DSS solution to drink for 7 days, followed by regular water for the following 5 days.
36615338	4	55	theme	DSS	469:471	arg1	solution					473:480	a 4% (w/v) DSS solution	458:480	a 4% (w/v) DSS solution to drink	458:489	Mice were given a 4% (w/v) DSS solution to drink for 7 days, followed by regular water for the following 5 days.
36615338	1	56	contain	have	197:200	arg1	Quercetin					114:122	Quercetin	114:122	Quercetin	114:122	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	56	contain	have	197:200	arg1	flavonoid					127:135	a flavonoid	125:135	a flavonoid that is present in vegetables and fruits	125:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	56	contain	have	197:200	arg1	present					145:151	present	145:151	present	145:151	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	56	contain	have	197:200	arg2	effects					220:226	anti-inflammatory effects	202:226	anti-inflammatory effects	202:226	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	8	57	theme	weight	905:910	arg1	loss					912:915	DSS-induced body weight loss	888:915	DSS-induced body weight loss	888:915	Quercetin attenuated DSS-induced body weight loss, colon length shortening, and pathological damage to the colon.
36615338	7	58	link	Enzyme-linked	732:744	arg1	assay					760:764	Enzyme-linked immunosorbent assay	732:764	Enzyme-linked immunosorbent assay	732:764	Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon.
36615338	12	59	theme	PI3K/AKT	1593:1600	arg1	signaling					1602:1610	PI3K/AKT signaling	1593:1610	PI3K/AKT signaling	1593:1610	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	3	60	dep	effect	327:332	arg1	the					323:325	the	323:325	the	323:325	This study aimed to explore the effect and pharmacological mechanism of quercetin on dextran sodium sulfate (DSS)-induced ulcerative colitis (UC).
36615338	8	61	theme	body	900:903	arg1	loss					912:915	DSS-induced body weight loss	888:915	DSS-induced body weight loss	888:915	Quercetin attenuated DSS-induced body weight loss, colon length shortening, and pathological damage to the colon.
36615338	8	62	theme	length	924:929	arg1	shortening					931:940	colon length shortening	918:940	colon length shortening	918:940	Quercetin attenuated DSS-induced body weight loss, colon length shortening, and pathological damage to the colon.
36615338	4	63	theme	regular	515:521	arg1	water					523:527	regular water	515:527	regular water for the following 5 days	515:552	Mice were given a 4% (w/v) DSS solution to drink for 7 days, followed by regular water for the following 5 days.
36615338	9	64	theme	bacteria	1117:1124	arg1	growth					1099:1104	the growth	1095:1104	the growth of harmful bacteria	1095:1124	Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria.
36615338	12	65	theme	microbiota	1491:1500	arg1	re-establishment					1503:1518	re-establishment	1503:1518	re-establishment of healthy microbiomes that favor mucosal healing	1503:1568	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	65	theme	microbiota	1491:1500	arg1	inhibition					1579:1588	the inhibition	1575:1588	the inhibition of PI3K/AKT signaling	1575:1610	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	65	theme	microbiota	1491:1500	arg1	regulation					1462:1471	regulation	1462:1471	regulation of the intestinal microbiota	1462:1500	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	1	66	from	fruits	171:176	arg1	Quercetin					114:122	Quercetin	114:122	Quercetin	114:122	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	66	from	fruits	171:176	arg1	flavonoid					127:135	a flavonoid	125:135	a flavonoid that is present in vegetables and fruits	125:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	66	from	fruits	171:176	arg1	present					145:151	present	145:151	present	145:151	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	10	67	theme	neoplastic	1219:1228	arg1	processes					1230:1238	inflammatory and neoplastic processes	1202:1238	inflammatory and neoplastic processes	1202:1238	Network pharmacology revealed that quercetin target genes were enriched in inflammatory and neoplastic processes.
36615338	1	68	theme	anti-inflammatory	202:218	arg1	effects					220:226	anti-inflammatory effects	202:226	anti-inflammatory effects	202:226	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	0	69	theme	Experimental	56:67	arg1	Colitis					69:75	Experimental Colitis	56:75	Experimental Colitis	56:75	Efficacy and Mechanism of Quercetin in the Treatment of Experimental Colitis Using Network Pharmacology Analysis.
36615338	12	70	theme	intestinal	1480:1489	arg1	microbiota					1491:1500	the intestinal microbiota	1476:1500	the intestinal microbiota	1476:1500	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	12	71	theme	healthy	1523:1529	arg1	microbiomes					1531:1541	healthy microbiomes	1523:1541	healthy microbiomes that favor mucosal healing	1523:1568	Quercetin has a role in the treatment of UC, with pharmacological mechanisms that involve regulation of the intestinal microbiota, re-establishment of healthy microbiomes that favor mucosal healing, and the inhibition of PI3K/AKT signaling.
36615338	9	72	theme	Quercetin	981:989	arg1	administration					991:1004	Quercetin administration	981:1004	Quercetin administration	981:1004	Quercetin administration modulated the composition of the intestinal microbiota in DSS-induced mice and inhibited the growth of harmful bacteria.
36615338	10	73	theme	quercetin	1162:1170	arg1	genes					1179:1183	quercetin target genes	1162:1183	quercetin target genes	1162:1183	Network pharmacology revealed that quercetin target genes were enriched in inflammatory and neoplastic processes.
36615338	1	74	from	vegetables	156:165	arg1	Quercetin					114:122	Quercetin	114:122	Quercetin	114:122	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	74	from	vegetables	156:165	arg1	flavonoid					127:135	a flavonoid	125:135	a flavonoid that is present in vegetables and fruits	125:176	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	1	74	from	vegetables	156:165	arg1	present					145:151	present	145:151	present	145:151	Quercetin, a flavonoid that is present in vegetables and fruits, has been found to have anti-inflammatory effects.
36615338	7	75	theme	quercetin	843:851	arg1	role					835:838	the anti-inflammatory role	813:838	the anti-inflammatory role of quercetin in the colon	813:864	Enzyme-linked immunosorbent assay and western blotting were performed to examine the anti-inflammatory role of quercetin in the colon.
36161027	0	0	from	development	11:21	arg1	album					78:82	Chenopodium album	66:82	Chenopodium album	66:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	1	1	from	functions	279:287	arg1	plants					320:325	a few wild plants	309:325	a few wild plants	309:325	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	1	2	theme	few	311:313	arg1	plants					320:325	a few wild plants	309:325	a few wild plants	309:325	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	0	3	theme	bladders	54:61	arg1	development					11:21	development	11:21	development	11:21	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	3	theme	bladders	54:61	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	3	theme	bladders	54:61	arg1	structures					127:136	specialized epidermal structures	105:136	specialized epidermal structures that halophytes use to store and excrete excess salt	105:189	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	3	theme	bladders	54:61	arg1	response					37:44	the salt response	28:44	the salt response of salt bladders in Chenopodium album	28:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	3	4	theme	rectangular	626:636	arg1	cell					644:647	a rectangular stalk cell	624:647	a rectangular stalk cell	624:647	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	4	5	theme	development	801:811	arg1	stages					791:796	different stages	781:796	different stages of development	781:811	The polysaccharides of salt bladder cell wall showed dynamic distribution at different stages of development.
36161027	5	6	theme	Na+	847:849	arg1	accumulation					858:869	Na+ and K+ accumulation	847:869	Na+ and K+ accumulation	847:869	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	0	7	from	Structure	0:8	arg1	album					78:82	Chenopodium album	66:82	Chenopodium album	66:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	6	8	theme	molecular	1221:1229	arg1	level					1231:1235	the molecular level	1217:1235	the molecular level	1217:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	9	theme	mechanisms	1103:1112	arg1	knowledge					1072:1080	our knowledge	1068:1080	our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level	1068:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	1	10	theme	salt	234:237	arg1	development					247:257	salt bladder development	234:257	salt bladder development	234:257	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	0	11	theme	Chenopodium	66:76	arg1	album					78:82	Chenopodium album	66:82	Chenopodium album	66:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	12	from	response	37:44	arg1	album					78:82	Chenopodium album	66:82	Chenopodium album	66:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	6	13	theme	desert	1145:1150	arg1	reference					1183:1191	a reference	1181:1191	a reference for further research at the molecular level	1181:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	13	theme	desert	1145:1150	arg1	plants					1152:1157	desert plants	1145:1157	desert plants	1145:1157	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	1	14	theme	bladder	239:245	arg1	development					247:257	salt bladder development	234:257	salt bladder development	234:257	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	0	15	theme	Salt	87:90	arg1	bladders					92:99	L. Salt bladders	84:99	L. Salt bladders	84:99	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	1	16	theme	wild	315:318	arg1	plants					320:325	a few wild plants	309:325	a few wild plants	309:325	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	4	17	theme	different	781:789	arg1	stages					791:796	different stages	781:796	different stages of development	781:811	The polysaccharides of salt bladder cell wall showed dynamic distribution at different stages of development.
36161027	6	18	from	structures	1131:1140	arg1	reference					1183:1191	a reference	1181:1191	a reference for further research at the molecular level	1181:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	18	from	structures	1131:1140	arg1	plants					1152:1157	desert plants	1145:1157	desert plants	1145:1157	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	0	19	theme	L.	84:85	arg1	bladders					92:99	L. Salt bladders	84:99	L. Salt bladders	84:99	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	20	theme	excess	179:184	arg1	salt					186:189	excess salt	179:189	excess salt	179:189	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	6	21	theme	structures	1131:1140	arg1	mechanisms					1103:1112	the physiological mechanisms	1085:1112	the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level	1085:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	22	from	plants	1152:1157	arg1	mechanisms					1103:1112	the physiological mechanisms	1085:1112	the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level	1085:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	23	theme	further	1197:1203	arg1	research					1205:1212	further research	1197:1212	further research at the molecular level	1197:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	24	theme	accessory	1121:1129	arg1	structures					1131:1140	the accessory structures	1117:1140	the accessory structures in desert plants, which can be used as a reference for further research at the molecular level	1117:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	3	25	theme	shoot	672:676	arg1	tip					678:680	the shoot tip	668:680	the shoot tip	668:680	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	1	26	theme	bladders	297:304	arg1	functions					279:287	the functions	275:287	the functions of salt bladders in a few wild plants	275:325	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	5	27	from	osmoregulation	935:948	arg1	leaves					983:988	leaves	983:988	leaves	983:988	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	5	28	theme	reactive	882:889	arg1	species					898:904	reactive oxygen species	882:904	reactive oxygen species scavenging	882:915	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	2	29	theme	bladder	389:395	arg1	development					397:407	salt bladder development	384:407	salt bladder development	384:407	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	4	30	theme	cell	740:743	arg1	wall					745:748	salt bladder cell wall	727:748	salt bladder cell wall	727:748	The polysaccharides of salt bladder cell wall showed dynamic distribution at different stages of development.
36161027	2	31	theme	Chenopodium	510:520	arg1	plants					528:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	5	32	theme	oxygen	891:896	arg1	species					898:904	reactive oxygen species	882:904	reactive oxygen species scavenging	882:915	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	6	33	from	mechanisms	1103:1112	arg1	reference					1183:1191	a reference	1181:1191	a reference for further research at the molecular level	1181:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	33	from	mechanisms	1103:1112	arg1	plants					1152:1157	desert plants	1145:1157	desert plants	1145:1157	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	2	34	theme	salt	384:387	arg1	development					397:407	salt bladder development	384:407	salt bladder development	384:407	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	2	35	theme	unbrushed	500:508	arg1	plants					528:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	5	36	theme	species	898:904	arg1	scavenging					906:915	reactive oxygen species scavenging	882:915	reactive oxygen species scavenging	882:915	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	5	37	from	efficiency	969:978	arg1	leaves					983:988	leaves	983:988	leaves	983:988	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	5	38	theme	photosynthetic	954:967	arg1	efficiency					969:978	photosynthetic efficiency	954:978	photosynthetic efficiency	954:978	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	0	39	theme	epidermal	117:125	arg1	development					11:21	development	11:21	development	11:21	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	39	theme	epidermal	117:125	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	39	theme	epidermal	117:125	arg1	structures					127:136	specialized epidermal structures	105:136	specialized epidermal structures that halophytes use to store and excrete excess salt	105:189	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	39	theme	epidermal	117:125	arg1	response					37:44	the salt response	28:44	the salt response of salt bladders in Chenopodium album	28:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	2	40	theme	present	361:367	arg1	study					369:373	the present study	357:373	the present study	357:373	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	0	41	theme	specialized	105:115	arg1	development					11:21	development	11:21	development	11:21	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	41	theme	specialized	105:115	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	41	theme	specialized	105:115	arg1	structures					127:136	specialized epidermal structures	105:136	specialized epidermal structures that halophytes use to store and excrete excess salt	105:189	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	41	theme	specialized	105:115	arg1	response					37:44	the salt response	28:44	the salt response of salt bladders in Chenopodium album	28:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	5	42	theme	plants	1036:1041	arg1	tolerance					1023:1031	the salt tolerance	1014:1031	the salt tolerance of plants	1014:1041	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	3	43	theme	bladder	554:560	arg1	cell					562:565	the bladder cell	550:565	the bladder cell of C. album	550:577	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	2	44	theme	bladder-brushed	480:494	arg1	plants					528:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	2	45	theme	salt	455:458	arg1	stress					460:465	salt stress	455:465	salt stress	455:465	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	2	46	theme	wall	415:418	arg1	composition					420:430	cell wall composition	410:430	cell wall composition	410:430	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	3	47	dep	young	690:694	arg1	leaves					696:701	leaves	696:701	leaves	696:701	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	6	48	theme	physiological	1089:1101	arg1	mechanisms					1103:1112	the physiological mechanisms	1085:1112	the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level	1085:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	0	49	theme	salt	32:35	arg1	development					11:21	development	11:21	development	11:21	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	49	theme	salt	32:35	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	49	theme	salt	32:35	arg1	structures					127:136	specialized epidermal structures	105:136	specialized epidermal structures that halophytes use to store and excrete excess salt	105:189	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	0	49	theme	salt	32:35	arg1	response					37:44	the salt response	28:44	the salt response of salt bladders in Chenopodium album	28:82	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	2	50	theme	cell	410:413	arg1	composition					420:430	cell wall composition	410:430	cell wall composition	410:430	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	4	51	theme	dynamic	757:763	arg1	distribution					765:776	dynamic distribution	757:776	dynamic distribution	757:776	The polysaccharides of salt bladder cell wall showed dynamic distribution at different stages of development.
36161027	5	52	theme	K+	855:856	arg1	accumulation					858:869	Na+ and K+ accumulation	847:869	Na+ and K+ accumulation	847:869	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	4	53	theme	bladder	732:738	arg1	wall					745:748	salt bladder cell wall	727:748	salt bladder cell wall	727:748	The polysaccharides of salt bladder cell wall showed dynamic distribution at different stages of development.
36161027	4	54	theme	salt	727:730	arg1	wall					745:748	salt bladder cell wall	727:748	salt bladder cell wall	727:748	The polysaccharides of salt bladder cell wall showed dynamic distribution at different stages of development.
36161027	3	55	theme	C.	570:571	arg1	album					573:577	C. album	570:577	C. album	570:577	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	4	56	theme	wall	745:748	arg1	polysaccharides					708:722	The polysaccharides	704:722	The polysaccharides of salt bladder cell wall	704:748	The polysaccharides of salt bladder cell wall showed dynamic distribution at different stages of development.
36161027	5	57	dep	osmoregulation	935:948	arg1	the					931:933	the	931:933	the	931:933	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	2	58	theme	album	522:526	arg1	plants					528:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	bladder-brushed and unbrushed Chenopodium album plants	480:533	Therefore, the present study examined salt bladder development, cell wall composition, and their roles under salt stress by employing bladder-brushed and unbrushed Chenopodium album plants.
36161027	3	59	theme	epidermal	600:608	arg1	cells					610:614	the epidermal cells	596:614	the epidermal cells	596:614	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	3	60	theme	album	573:577	arg1	cell					562:565	the bladder cell	550:565	the bladder cell of C. album	550:577	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	0	61	theme	salt	49:52	arg1	bladders					54:61	salt bladders	49:61	salt bladders	49:61	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	5	62	theme	salt	1018:1021	arg1	tolerance					1023:1031	the salt tolerance	1014:1031	the salt tolerance of plants	1014:1041	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	3	63	theme	stalk	638:642	arg1	cell					644:647	a rectangular stalk cell	624:647	a rectangular stalk cell	624:647	We found that the bladder cell of C. album was connected to the epidermal cells through a rectangular stalk cell and developed from the shoot tip and the young leaves.
36161027	5	64	theme	salt	824:827	arg1	bladders					829:836	salt bladders	824:836	salt bladders	824:836	Moreover, salt bladders affected Na+ and K+ accumulation, increased reactive oxygen species scavenging, and improved the osmoregulation and photosynthetic efficiency in leaves, subsequently enhancing the salt tolerance of plants.
36161027	6	65	from	level	1231:1235	arg1	research					1205:1212	further research	1197:1212	further research at the molecular level	1197:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	1	66	theme	cell	205:208	arg1	unclear					262:268	unclear	262:268	unclear	262:268	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	1	66	theme	cell	205:208	arg1	composition					215:225	the cell wall composition	201:225	the cell wall composition during salt bladder development	201:257	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	1	67	theme	salt	292:295	arg1	bladders					297:304	salt bladders	292:304	salt bladders	292:304	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	0	68	dep	Structure	0:8	arg1	bladders					92:99	L. Salt bladders	84:99	L. Salt bladders	84:99	Structure, development, and the salt response of salt bladders in Chenopodium album L. Salt bladders are specialized epidermal structures that halophytes use to store and excrete excess salt.
36161027	6	69	used	used	1173:1176	arg2	plants					1152:1157	desert plants	1145:1157	desert plants	1145:1157	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	6	69	used	used	1173:1176	arg2	reference					1183:1191	a reference	1181:1191	a reference for further research at the molecular level	1181:1235	The findings strengthen our knowledge of the physiological mechanisms of the accessory structures in desert plants, which can be used as a reference for further research at the molecular level.
36161027	1	70	theme	wall	210:213	arg1	unclear					262:268	unclear	262:268	unclear	262:268	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
36161027	1	70	theme	wall	210:213	arg1	composition					215:225	the cell wall composition	201:225	the cell wall composition during salt bladder development	201:257	However, the cell wall composition during salt bladder development is unclear, and the functions of salt bladders in a few wild plants remain unexplored.
35647094	6	0	theme	Th2	924:926	arg1	responses					935:943	both Th1 and Th2 immune responses	911:943	both Th1 and Th2 immune responses	911:943	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	7	1	theme	healthier	1391:1399	arg1	composition					1423:1433	a healthier intestinal microbiota composition	1389:1433	a healthier intestinal microbiota composition for the host	1389:1446	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	8	2	used	used	1546:1549	arg2	alternative					1557:1567	an alternative	1554:1567	an alternative to prophylactic antibiotics in poultry feeding	1554:1614	Taken together, it can be concluded that GPS is safe to use as a novel feed additive that can be used as an alternative to prophylactic antibiotics in poultry feeding.
35647094	8	2	used	used	1546:1549	arg2	additive					1525:1532	additive	1525:1532	additive	1525:1532	Taken together, it can be concluded that GPS is safe to use as a novel feed additive that can be used as an alternative to prophylactic antibiotics in poultry feeding.
35647094	4	3	from	effects	612:618	arg1	intestines					638:647	poultry intestines	630:647	poultry intestines	630:647	The present study investigated the pharmacological effects of GPS on poultry intestines to assess whether it can be used as a feed additive.
35647094	1	4	theme	drug	160:163	arg1	residue					165:171	drug residue	160:171	drug residue in animals' bodies	160:190	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	7	5	theme	gene	1120:1123	arg1	sequencing					1125:1134	16S rRNA gene sequencing	1111:1134	16S rRNA gene sequencing	1111:1134	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	0	6	from	Effects	0:6	arg1	Chickens					42:49	Chickens	42:49	Chickens	42:49	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	7	7	theme	decreased	1333:1341	arg1	portion					1343:1349	a decreased portion	1331:1349	intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium	1179:1375	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	4	8	theme	pharmacological	596:610	arg1	effects					612:618	the pharmacological effects	592:618	the pharmacological effects of GPS on poultry intestines	592:647	The present study investigated the pharmacological effects of GPS on poultry intestines to assess whether it can be used as a feed additive.
35647094	7	9	theme	sequencing	1125:1134	arg1	results					1100:1106	the results	1096:1106	the results of 16S rRNA gene sequencing	1096:1134	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	7	10	theme	intestinal	1401:1410	arg1	composition					1423:1433	a healthier intestinal microbiota composition	1389:1433	a healthier intestinal microbiota composition for the host	1389:1446	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	3	11	theme	previous	543:550	arg1	studies					552:558	previous studies	543:558	previous studies	543:558	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	1	12	dep	causes	133:138	arg1	endanger					302:309	endanger	302:309	not only compromise animals' health but ultimately endanger human health	251:322	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	1	12	dep	causes	133:138	arg1	compromise					260:269	compromise	260:269	not only compromise animals' health but ultimately endanger human health	251:322	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	7	13	theme	16S	1111:1113	arg1	sequencing					1125:1134	16S rRNA gene sequencing	1111:1134	16S rRNA gene sequencing	1111:1134	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	7	14	theme	microbiota	1412:1421	arg1	composition					1423:1433	a healthier intestinal microbiota composition	1389:1433	a healthier intestinal microbiota composition for the host	1389:1446	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	1	15	from	accumulation	144:155	arg1	bodies					185:190	animals' bodies	176:190	animals' bodies	176:190	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	3	16	theme	immunomodulatory	515:530	arg1	effects					532:538	promising immunomodulatory effects	505:538	promising immunomodulatory effects	505:538	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	7	17	theme	increased	1248:1256	arg1	proportion					1258:1267	the increased proportion	1244:1267	the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella	1244:1317	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	1	18	theme	residue	165:171	arg1	accumulation					144:155	the accumulation	140:155	the accumulation of drug residue in animals' bodies	140:190	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	1	18	theme	residue	165:171	arg1	occurrence					200:209	the occurrence	196:209	the occurrence of antibiotic-resistant bacteria	196:242	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	7	19	theme	rRNA	1115:1118	arg1	sequencing					1125:1134	16S rRNA gene sequencing	1111:1134	16S rRNA gene sequencing	1111:1134	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	5	20	theme	short-chain	868:878	arg1	acids					886:890	short-chain fatty acids	868:890	short-chain fatty acids	868:890	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	7	21	from	composition	1201:1211	arg1	intestine					1220:1228	the intestine	1216:1228	the intestine	1216:1228	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	7	22	theme	Eisenbergiella	1304:1317	arg1	proportion					1258:1267	the increased proportion	1244:1267	the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella	1244:1317	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	5	23	theme	sIgA	755:758	arg1	production					741:750	production	741:750	production of sIgA	741:758	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	7	24	theme	intestinal	1179:1188	arg1	composition					1201:1211	intestinal microbiota composition	1179:1211	intestinal microbiota composition	1179:1211	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	6	25	theme	cells	1062:1066	arg1	proportion					1029:1038	the proportion	1025:1038	the proportion of both CD4+ and CD8+ cells in the intestine	1025:1083	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	4	26	used	used	677:680	arg2	it					667:668	it	667:668	it	667:668	The present study investigated the pharmacological effects of GPS on poultry intestines to assess whether it can be used as a feed additive.
35647094	4	26	used	used	677:680	arg2	additive					692:699	additive	692:699	additive	692:699	The present study investigated the pharmacological effects of GPS on poultry intestines to assess whether it can be used as a feed additive.
35647094	6	27	theme	IFN-γ	1002:1006	arg1	expression					965:974	the expression	961:974	the expression of IL-2, IL-4, IL-1β, and IFN-γ	961:1006	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	2	28	theme	excessive	407:415	arg1	antibiotics					417:427	excessive antibiotics	407:427	excessive antibiotics	407:427	Thus, there is an urgent need for a novel poultry feed additive to substitute for excessive antibiotics.
35647094	6	29	theme	immune	928:933	arg1	responses					935:943	both Th1 and Th2 immune responses	911:943	both Th1 and Th2 immune responses	911:943	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	5	30	from	changes	857:863	arg1	acids					886:890	short-chain fatty acids	868:890	short-chain fatty acids	868:890	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	0	31	theme	Glycyrrhiza	11:21	arg1	Polysaccharides					23:37	Glycyrrhiza Polysaccharides	11:37	Glycyrrhiza Polysaccharides	11:37	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	4	32	theme	present	565:571	arg1	study					573:577	The present study	561:577	The present study	561:577	The present study investigated the pharmacological effects of GPS on poultry intestines to assess whether it can be used as a feed additive.
35647094	6	33	theme	IL-2	979:982	arg1	expression					965:974	the expression	961:974	the expression of IL-2, IL-4, IL-1β, and IFN-γ	961:1006	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	1	34	theme	antibiotics	102:112	arg1	overuse					91:97	The overuse	87:97	The overuse of antibiotics in poultry farming	87:131	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	4	35	theme	poultry	630:636	arg1	intestines					638:647	poultry intestines	630:647	poultry intestines	630:647	The present study investigated the pharmacological effects of GPS on poultry intestines to assess whether it can be used as a feed additive.
35647094	3	36	theme	Chinese	477:483	arg1	licorice					485:492	Chinese licorice	477:492	Chinese licorice	477:492	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	6	37	theme	IL-1β	991:995	arg1	expression					965:974	the expression	961:974	the expression of IL-2, IL-4, IL-1β, and IFN-γ	961:1006	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	8	38	theme	poultry	1600:1606	arg1	feeding					1608:1614	poultry feeding	1600:1614	poultry feeding	1600:1614	Taken together, it can be concluded that GPS is safe to use as a novel feed additive that can be used as an alternative to prophylactic antibiotics in poultry feeding.
35647094	3	39	theme	Glycyrrhiza	430:440	arg1	polysaccharides					442:456	Glycyrrhiza polysaccharides	430:456	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice	430:492	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	3	39	theme	Glycyrrhiza	430:440	arg1	GPS					459:461	GPS	459:461	GPS	459:461	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	5	40	theme	microbial	823:831	arg1	composition					833:843	the gut microbial composition	815:843	the gut microbial composition	815:843	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	6	41	theme	Th1	916:918	arg1	responses					935:943	both Th1 and Th2 immune responses	911:943	both Th1 and Th2 immune responses	911:943	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	0	42	theme	Polysaccharides	23:37	arg1	Homeostasis					74:84	Homeostasis	74:84	Homeostasis	74:84	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	0	42	theme	Polysaccharides	23:37	arg1	Effects					0:6	Effects	0:6	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.	0:85	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	0	42	theme	Polysaccharides	23:37	arg1	Health					63:68	' Intestinal Health	50:68	' Intestinal Health	50:68	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	5	43	theme	goblet	795:800	arg1	cells					802:806	goblet cells	795:806	goblet cells	795:806	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	0	44	theme	Intestinal	52:61	arg1	Health					63:68	' Intestinal Health	50:68	' Intestinal Health	50:68	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	0	44	theme	Intestinal	52:61	arg1	Effects					0:6	Effects	0:6	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.	0:85	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	1	45	from	occurrence	200:209	arg1	bodies					185:190	animals' bodies	176:190	animals' bodies	176:190	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	5	46	theme	fatty	880:884	arg1	acids					886:890	short-chain fatty acids	868:890	short-chain fatty acids	868:890	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	5	47	theme	secretion	773:781	arg1	activity					783:790	the secretion activity	769:790	the secretion activity of goblet cells	769:806	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	1	48	theme	human	311:315	arg1	health					317:322	human health	311:322	human health	311:322	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	8	49	from	antibiotics	1585:1595	arg1	feeding					1608:1614	poultry feeding	1600:1614	poultry feeding	1600:1614	Taken together, it can be concluded that GPS is safe to use as a novel feed additive that can be used as an alternative to prophylactic antibiotics in poultry feeding.
35647094	1	50	from	bodies	185:190	arg1	accumulation					144:155	the accumulation	140:155	the accumulation of drug residue in animals' bodies	140:190	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	1	50	from	bodies	185:190	arg1	occurrence					200:209	the occurrence	196:209	the occurrence of antibiotic-resistant bacteria	196:242	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	1	51	theme	poultry	117:123	arg1	farming					125:131	poultry farming	117:131	poultry farming	117:131	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	7	52	theme	microbiota	1190:1199	arg1	composition					1201:1211	intestinal microbiota composition	1179:1211	intestinal microbiota composition	1179:1211	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	7	53	theme	Butyricicoccus	1285:1298	arg1	proportion					1258:1267	the increased proportion	1244:1267	the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella	1244:1317	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	7	54	theme	Erysipelatoclostridium	1354:1375	arg1	portion					1343:1349	a decreased portion	1331:1349	intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium	1179:1375	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	7	54	theme	Erysipelatoclostridium	1354:1375	arg1	composition					1201:1211	intestinal microbiota composition	1179:1211	intestinal microbiota composition	1179:1211	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	6	55	theme	CD8+	1057:1060	arg1	cells					1062:1066	both CD4+ and CD8+ cells	1043:1066	both CD4+ and CD8+ cells	1043:1066	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	3	56	attach	derived	464:470	arg2	GPS					459:461	GPS	459:461	GPS	459:461	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	3	56	attach	derived	464:470	arg2	polysaccharides					442:456	Glycyrrhiza polysaccharides	430:456	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice	430:492	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	3	56	attach	derived	464:470	arg1	licorice					485:492	Chinese licorice	477:492	Chinese licorice	477:492	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	6	57	theme	IL-4	985:988	arg1	expression					965:974	the expression	961:974	the expression of IL-2, IL-4, IL-1β, and IFN-γ	961:1006	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	5	58	theme	cells	802:806	arg1	activity					783:790	the secretion activity	769:790	the secretion activity of goblet cells	769:806	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	3	59	theme	promising	505:513	arg1	effects					532:538	promising immunomodulatory effects	505:538	promising immunomodulatory effects	505:538	Glycyrrhiza polysaccharides (GPS) derived from Chinese licorice have shown promising immunomodulatory effects in previous studies.
35647094	7	60	from	portion	1343:1349	arg1	intestine					1220:1228	the intestine	1216:1228	the intestine	1216:1228	Moreover, the results of 16S rRNA gene sequencing showed that GPS could significantly change intestinal microbiota composition in the intestine, evidenced by the increased proportion of Bacteroides, Butyricicoccus and Eisenbergiella, as well as a decreased portion of Erysipelatoclostridium, leading to a healthier intestinal microbiota composition for the host.
35647094	1	61	theme	antibiotic-resistant	214:233	arg1	bacteria					235:242	antibiotic-resistant bacteria	214:242	antibiotic-resistant bacteria	214:242	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	5	62	theme	gut	819:821	arg1	composition					833:843	the gut microbial composition	815:843	the gut microbial composition	815:843	The results show that GPS can increase production of sIgA, promote the secretion activity of goblet cells, alter the gut microbial composition and lead to changes in short-chain fatty acids.
35647094	8	63	theme	prophylactic	1572:1583	arg1	antibiotics					1585:1595	prophylactic antibiotics	1572:1595	prophylactic antibiotics in poultry feeding	1572:1614	Taken together, it can be concluded that GPS is safe to use as a novel feed additive that can be used as an alternative to prophylactic antibiotics in poultry feeding.
35647094	6	64	from	proportion	1029:1038	arg1	intestine					1075:1083	the intestine	1071:1083	the intestine	1071:1083	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	6	65	theme	CD4+	1048:1051	arg1	cells					1062:1066	both CD4+ and CD8+ cells	1043:1066	both CD4+ and CD8+ cells	1043:1066	GPS also elevated both Th1 and Th2 immune responses by facilitating the expression of IL-2, IL-4, IL-1β, and IFN-γ while increasing the proportion of both CD4+ and CD8+ cells in the intestine.
35647094	1	66	from	overuse	91:97	arg1	farming					125:131	poultry farming	117:131	poultry farming	117:131	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	1	67	theme	bacteria	235:242	arg1	accumulation					144:155	the accumulation	140:155	the accumulation of drug residue in animals' bodies	140:190	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	1	67	theme	bacteria	235:242	arg1	occurrence					200:209	the occurrence	196:209	the occurrence of antibiotic-resistant bacteria	196:242	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	0	68	dep	Effects	0:6	arg1	Homeostasis					74:84	Homeostasis	74:84	Homeostasis	74:84	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	0	68	dep	Effects	0:6	arg1	Health					63:68	' Intestinal Health	50:68	' Intestinal Health	50:68	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	0	68	dep	Effects	0:6	arg1	Effects					0:6	Effects	0:6	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.	0:85	Effects of Glycyrrhiza Polysaccharides on Chickens' Intestinal Health and Homeostasis.
35647094	1	69	from	residue	165:171	arg1	bodies					185:190	animals' bodies	176:190	animals' bodies	176:190	The overuse of antibiotics in poultry farming causes the accumulation of drug residue in animals' bodies and the occurrence of antibiotic-resistant bacteria, which not only compromise animals' health but ultimately endanger human health.
35647094	2	70	theme	urgent	343:348	arg1	need					350:353	an urgent need	340:353	an urgent need for a novel poultry feed additive to substitute for excessive antibiotics	340:427	Thus, there is an urgent need for a novel poultry feed additive to substitute for excessive antibiotics.
35647094	4	71	theme	GPS	623:625	arg1	effects					612:618	the pharmacological effects	592:618	the pharmacological effects of GPS on poultry intestines	592:647	The present study investigated the pharmacological effects of GPS on poultry intestines to assess whether it can be used as a feed additive.
36565722	0	0	theme	tube	84:87	arg1	stages					89:94	spore and germ tube stages	69:94	stages	89:94	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	2	1	theme	recognition	467:477	arg1	receptors					479:487	pattern recognition receptors	459:487	pattern recognition receptors	459:487	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	3	2	theme	Lichtheimia	697:707	arg1	corymbifera					709:719	Lichtheimia corymbifera	697:719	Lichtheimia corymbifera	697:719	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	2	3	theme	90	347:348	arg1	%					349:349	%	349:349	%	349:349	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	0	4	theme	germ	79:82	arg1	stages					89:94	spore and germ tube stages	69:94	stages	89:94	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	3	5	theme	wall	674:677	arg1	composition					650:660	the precise composition	638:660	the precise composition of the cell wall of two strains of Lichtheimia corymbifera	638:719	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	5	6	theme	germination	1081:1091	arg1	stage					1093:1097	the germination stage	1077:1097	the germination stage	1077:1097	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	6	7	theme	swollen	1221:1227	arg1	spores					1229:1234	swollen spores	1221:1234	swollen spores	1221:1234	Immunofluorescence studies confirmed the exposure of β-glucan on the surface of swollen spores and germ-tubes.
36565722	6	8	theme	germ-tubes	1240:1249	arg1	surface					1210:1216	the surface	1206:1216	the surface of swollen spores and germ-tubes	1206:1249	Immunofluorescence studies confirmed the exposure of β-glucan on the surface of swollen spores and germ-tubes.
36565722	3	9	theme	corymbifera	709:719	arg1	strains					686:692	two strains	682:692	two strains of Lichtheimia corymbifera	682:719	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	10	theme	emerging	532:539	arg1	infection					558:566	an emerging life-threatening infection	529:566	an emerging life-threatening infection caused by the species of the order Mucorales	529:611	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	10	theme	emerging	532:539	arg1	worldwide					627:635	increasing worldwide	616:635	increasing worldwide	616:635	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	1	11	dep	composition	142:152	arg1	The					128:130	The	128:130	The	128:130	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	7	12	theme	corymbifera	1285:1295	arg1	germ-tubes					1268:1277	germ-tubes	1268:1277	germ-tubes	1268:1277	Both spores and germ-tubes of L. corymbifera promoted an early and strong pro-inflammatory response, through TLR-2.
36565722	7	12	theme	corymbifera	1285:1295	arg1	spores					1257:1262	spores	1257:1262	spores	1257:1262	Both spores and germ-tubes of L. corymbifera promoted an early and strong pro-inflammatory response, through TLR-2.
36565722	6	13	theme	Immunofluorescence	1141:1158	arg1	studies					1160:1166	Immunofluorescence studies	1141:1166	Immunofluorescence studies	1141:1166	Immunofluorescence studies confirmed the exposure of β-glucan on the surface of swollen spores and germ-tubes.
36565722	7	14	theme	pro-inflammatory	1326:1341	arg1	response					1343:1350	an early and strong pro-inflammatory response	1306:1350	an early and strong pro-inflammatory response	1306:1350	Both spores and germ-tubes of L. corymbifera promoted an early and strong pro-inflammatory response, through TLR-2.
36565722	3	15	theme	mucormycosis	515:526	arg1	incidence					502:510	the incidence	498:510	the incidence of mucormycosis	498:526	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	0	16	theme	host-pathogen	100:112	arg1	interactions					114:125	host-pathogen interactions	100:125	host-pathogen interactions	100:125	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	3	17	dep	germination	768:778	arg1	germ-tubes					792:801	germ-tubes	792:801	germ-tubes	792:801	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	17	dep	germination	768:778	arg1	spores					781:786	spores	781:786	spores	781:786	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	1	18	theme	cell	189:192	arg1	wall					194:197	the cell wall	185:197	the cell wall of filamentous fungi	185:218	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	2	19	theme	pattern	459:465	arg1	receptors					479:487	pattern recognition receptors	459:487	pattern recognition receptors	459:487	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	2	20	theme	fungal	317:322	arg1	wall					329:332	the fungal cell wall	313:332	the fungal cell wall	313:332	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	8	21	theme	opening	1442:1448	arg1	Mucorales					1431:1439	the order Mucorales	1421:1439	the order Mucorales	1421:1439	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	8	21	theme	opening	1442:1448	arg1	perspectives					1450:1461	opening perspectives	1442:1461	opening perspectives for the development of specific diagnostic biomarkers	1442:1515	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	1	22	theme	wall	194:197	arg1	composition					142:152	molecular composition	132:152	molecular composition	132:152	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	1	22	theme	wall	194:197	arg1	organization					169:180	structural organization	158:180	structural organization	158:180	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	0	23	theme	Parietal	0:7	arg1	composition					9:19	Parietal composition	0:19	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.	0:126	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	0	24	theme	Lichtheimia	24:34	arg1	corymbifera					36:46	Lichtheimia corymbifera	24:46	Lichtheimia corymbifera	24:46	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	3	25	theme	order	597:601	arg1	Mucorales					603:611	the order Mucorales	593:611	the order Mucorales	593:611	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	26	theme	life-threatening	541:556	arg1	infection					558:566	an emerging life-threatening infection	529:566	an emerging life-threatening infection caused by the species of the order Mucorales	529:611	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	26	theme	life-threatening	541:556	arg1	worldwide					627:635	increasing worldwide	616:635	increasing worldwide	616:635	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	1	27	theme	filamentous	202:212	arg1	fungi					214:218	filamentous fungi	202:218	filamentous fungi	202:218	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	5	28	theme	monosaccharides	973:987	arg1	presence					952:959	the presence	948:959	the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage	948:1097	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	8	29	theme	cell	1408:1411	arg1	wall					1413:1416	the cell wall	1404:1416	the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers	1404:1515	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	1	30	theme	fungi	214:218	arg1	wall					194:197	the cell wall	185:197	the cell wall of filamentous fungi	185:218	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	3	31	theme	precise	642:648	arg1	composition					650:660	the precise composition	638:660	the precise composition of the cell wall of two strains of Lichtheimia corymbifera	638:719	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	8	32	theme	wall	1413:1416	arg1	singularity					1389:1399	the singularity	1385:1399	the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers	1385:1515	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	7	33	theme	early	1309:1313	arg1	response					1343:1350	an early and strong pro-inflammatory response	1306:1350	an early and strong pro-inflammatory response	1306:1350	Both spores and germ-tubes of L. corymbifera promoted an early and strong pro-inflammatory response, through TLR-2.
36565722	0	34	theme	corymbifera	36:46	arg1	composition					9:19	Parietal composition	0:19	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.	0:126	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	2	35	theme	small	407:411	arg1	variations					413:422	small variations	407:422	small variations	407:422	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	3	36	theme	germination	768:778	arg1	stages					758:763	the early growth stages	741:763	the early growth stages of germination (spores and germ-tubes)	741:802	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	37	theme	early	745:749	arg1	stages					758:763	the early growth stages	741:763	the early growth stages of germination (spores and germ-tubes)	741:802	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	5	38	theme	uncommon	964:971	arg1	galactose					998:1006	galactose	998:1006	galactose	998:1006	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	38	theme	uncommon	964:971	arg1	acid					1024:1027	glucuronic acid	1013:1027	glucuronic acid	1013:1027	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	38	theme	uncommon	964:971	arg1	fucose					990:995	fucose	990:995	fucose	990:995	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	38	theme	uncommon	964:971	arg1	monosaccharides					973:987	uncommon monosaccharides	964:987	uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage	964:1097	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	39	dep	monosaccharides	973:987	arg1	galactose					998:1006	galactose	998:1006	galactose	998:1006	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	39	dep	monosaccharides	973:987	arg1	monosaccharides					973:987	uncommon monosaccharides	964:987	uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage	964:1097	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	39	dep	monosaccharides	973:987	arg1	fucose					990:995	fucose	990:995	fucose	990:995	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	39	dep	monosaccharides	973:987	arg1	acid					1024:1027	glucuronic acid	1013:1027	glucuronic acid	1013:1027	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	2	40	theme	%	349:349	arg1	polysaccharides					351:365	90% polysaccharides	347:365	90% polysaccharides	347:365	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	3	41	theme	growth	751:756	arg1	stages					758:763	the early growth stages	741:763	the early growth stages of germination (spores and germ-tubes)	741:802	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	5	42	theme	early	1110:1114	arg1	reorganization					1125:1138	early parietal reorganization	1110:1138	early parietal reorganization	1110:1138	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	43	theme	respective	1036:1045	arg1	proportions					1047:1057	respective proportions	1036:1057	respective proportions	1036:1057	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	8	44	theme	Mucorales	1431:1439	arg1	wall					1413:1416	the cell wall	1404:1416	the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers	1404:1515	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	4	45	theme	cells	907:911	arg1	response					889:896	the response	885:896	the response of THP-1 cells to Mucorales	885:924	This study also characterizes the response of THP-1 cells to Mucorales.
36565722	3	46	theme	cell	669:672	arg1	wall					674:677	the cell wall	665:677	the cell wall of two strains of Lichtheimia corymbifera	665:719	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	6	47	theme	spores	1229:1234	arg1	surface					1210:1216	the surface	1206:1216	the surface of swollen spores and germ-tubes	1206:1249	Immunofluorescence studies confirmed the exposure of β-glucan on the surface of swollen spores and germ-tubes.
36565722	5	48	theme	glucuronic	1013:1022	arg1	monosaccharides					973:987	uncommon monosaccharides	964:987	uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage	964:1097	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	5	48	theme	glucuronic	1013:1022	arg1	acid					1024:1027	glucuronic acid	1013:1027	glucuronic acid	1013:1027	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	1	49	theme	molecular	132:140	arg1	composition					142:152	molecular composition	132:152	molecular composition	132:152	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	4	50	theme	THP-1	901:905	arg1	cells					907:911	THP-1 cells	901:911	THP-1 cells	901:911	This study also characterizes the response of THP-1 cells to Mucorales.
36565722	8	51	theme	biomarkers	1506:1515	arg1	development					1471:1481	the development	1467:1481	the development of specific diagnostic biomarkers	1467:1515	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	3	52	theme	increasing	616:625	arg1	infection					558:566	an emerging life-threatening infection	529:566	an emerging life-threatening infection caused by the species of the order Mucorales	529:611	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	52	theme	increasing	616:625	arg1	worldwide					627:635	increasing worldwide	616:635	increasing worldwide	616:635	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	2	53	from	fungus	388:393	arg1	similar					371:377	similar	371:377	similar	371:377	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	2	53	from	fungus	388:393	arg1	organization					297:308	the organization	293:308	the organization	293:308	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	2	54	theme	cell	324:327	arg1	wall					329:332	the fungal cell wall	313:332	the fungal cell wall	313:332	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	3	55	theme	confocal	834:841	arg1	microscopy					843:852	confocal microscopy	834:852	confocal microscopy	834:852	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	2	56	theme	wall	329:332	arg1	similar					371:377	similar	371:377	similar	371:377	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	2	56	theme	wall	329:332	arg1	organization					297:308	the organization	293:308	the organization	293:308	Although the organization of the fungal cell wall, composed of 90% polysaccharides, is similar from one fungus to another, small variations condition their ability to trigger pattern recognition receptors.
36565722	8	57	theme	order	1425:1429	arg1	Mucorales					1431:1439	the order Mucorales	1421:1439	the order Mucorales	1421:1439	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	8	57	theme	order	1425:1429	arg1	perspectives					1450:1461	opening perspectives	1442:1461	opening perspectives for the development of specific diagnostic biomarkers	1442:1515	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	8	58	theme	diagnostic	1495:1504	arg1	biomarkers					1506:1515	specific diagnostic biomarkers	1486:1515	specific diagnostic biomarkers	1486:1515	Our results show the singularity of the cell wall of the order Mucorales, opening perspectives for the development of specific diagnostic biomarkers.
36565722	0	59	dep	composition	9:19	arg1	Differences					49:59	Differences	49:59	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.	0:126	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	7	60	theme	strong	1319:1324	arg1	response					1343:1350	an early and strong pro-inflammatory response	1306:1350	an early and strong pro-inflammatory response	1306:1350	Both spores and germ-tubes of L. corymbifera promoted an early and strong pro-inflammatory response, through TLR-2.
36565722	6	61	theme	β-glucan	1194:1201	arg1	exposure					1182:1189	the exposure	1178:1189	the exposure of β-glucan	1178:1201	Immunofluorescence studies confirmed the exposure of β-glucan on the surface of swollen spores and germ-tubes.
36565722	0	62	theme	spore	69:73	arg1	stages					89:94	spore and germ tube stages	69:94	stages	89:94	Parietal composition of Lichtheimia corymbifera: Differences between spore and germ tube stages and host-pathogen interactions.
36565722	5	63	theme	parietal	1116:1123	arg1	reorganization					1125:1138	early parietal reorganization	1110:1138	early parietal reorganization	1110:1138	The study identified the presence of uncommon monosaccharides (fucose, galactose, and glucuronic acid) whose respective proportions vary according to the germination stage, revealing early parietal reorganization.
36565722	3	64	theme	Mucorales	603:611	arg1	species					582:588	the species	578:588	the species of the order Mucorales	578:611	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	3	65	theme	strains	686:692	arg1	wall					674:677	the cell wall	665:677	the cell wall of two strains of Lichtheimia corymbifera	665:719	Because the incidence of mucormycosis, an emerging life-threatening infection caused by the species of the order Mucorales is increasing worldwide, the precise composition of the cell wall of two strains of Lichtheimia corymbifera was investigated in the early growth stages of germination (spores and germ-tubes) using trimethylsilylation and confocal microscopy.
36565722	1	66	theme	structural	158:167	arg1	organization					169:180	structural organization	158:180	structural organization	158:180	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36565722	1	67	theme	host	248:251	arg1	ability					233:239	the ability	229:239	the ability of the host to identify them as pathogens	229:281	The molecular composition and structural organization of the cell wall of filamentous fungi underlie the ability of the host to identify them as pathogens.
36570240	3	0	theme	α-glucosidase	534:546	arg1	inhibition					496:505	inhibition	496:505	inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity	496:600	Moreover, inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity were investigated.
36570240	6	1	theme	enzyme	1210:1215	arg1	activity					1228:1235	reduced enzyme inhibition activity	1202:1235	reduced enzyme inhibition activity	1202:1235	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	3	2	theme	antioxidant	581:591	arg1	activity					593:600	their antioxidant activity	575:600	their antioxidant activity	575:600	Moreover, inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity were investigated.
36570240	5	3	from	Fermentata	1050:1059	arg1	activity					993:1000	the antioxidant activity	977:1000	the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata	977:1059	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	3	from	Fermentata	1050:1059	arg1	polysaccharides					1009:1023	the polysaccharides	1005:1023	the polysaccharides from Chao Massa Medicata Fermentata	1005:1059	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	3	from	Fermentata	1050:1059	arg1	higher					1065:1070	higher	1065:1070	higher	1065:1070	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	4	theme	polysaccharides	1009:1023	arg1	activity					993:1000	the antioxidant activity	977:1000	the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata	977:1059	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	4	theme	polysaccharides	1009:1023	arg1	higher					1065:1070	higher	1065:1070	higher	1065:1070	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	5	from	Fermentata	880:889	arg1	Polysaccharides					838:852	Polysaccharides	838:852	Polysaccharides from Sheng Massa Medicata Fermentata	838:889	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	6	6	from	activity	1228:1235	arg1	activity					1268:1275	antioxidant activity	1256:1275	antioxidant activity	1256:1275	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	6	7	theme	reduced	1202:1208	arg1	activity					1228:1235	reduced enzyme inhibition activity	1202:1235	reduced enzyme inhibition activity	1202:1235	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	4	8	theme	acid	785:788	arg1	content					790:796	higher uronic acid content	771:796	higher uronic acid content	771:796	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	9	theme	uronic	778:783	arg1	content					790:796	higher uronic acid content	771:796	higher uronic acid content	771:796	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	5	10	theme	trypsin	910:916	arg1	activity					959:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	3	11	theme	trypsin	510:516	arg1	inhibition					496:505	inhibition	496:505	inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity	496:600	Moreover, inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity were investigated.
36570240	4	12	theme	chains	830:835	arg1	weight					763:768	a lower molecular weight	745:768	a lower molecular weight	745:768	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	12	theme	chains	830:835	arg1	proportion					811:820	a lower proportion	803:820	a lower proportion of side chains	803:835	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	12	theme	chains	830:835	arg1	content					790:796	higher uronic acid content	771:796	higher uronic acid content	771:796	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	5	13	theme	α-glucosidase	934:946	arg1	inhibition					948:957	α-glucosidase inhibition	934:957	α-glucosidase inhibition	934:957	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	0	14	theme	Processed	113:121	arg1	Products					123:130	Its Processed Products	109:130	Its Processed Products	109:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	0	15	from	Properties	44:53	arg1	Fermentata					94:103	Fermentata	94:103	Fermentata	94:103	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	0	15	from	Properties	44:53	arg1	Products					123:130	Its Processed Products	109:130	Its Processed Products	109:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	4	16	theme	side	825:828	arg1	chains					830:835	side chains	825:835	side chains	825:835	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	17	from	Fermentata	730:739	arg1	polysaccharides					689:703	polysaccharides	689:703	polysaccharides from Chao Massa Medicata Fermentata	689:739	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	1	18	theme	different	197:205	arg1	types					207:211	different types	197:211	different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata)	197:329	Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata).
36570240	2	19	theme	physicochemical	336:350	arg1	properties					352:361	The physicochemical properties	332:361	The physicochemical properties of these polysaccharides	332:386	The physicochemical properties of these polysaccharides were studied, including the molecular weight, monosaccharide composition, and glycosidic linkage.
36570240	2	20	theme	molecular	416:424	arg1	weight					426:431	the molecular weight	412:431	the molecular weight	412:431	The physicochemical properties of these polysaccharides were studied, including the molecular weight, monosaccharide composition, and glycosidic linkage.
36570240	4	21	contain	had	741:743	arg2	content					790:796	higher uronic acid content	771:796	higher uronic acid content	771:796	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	21	contain	had	741:743	arg1	polysaccharides					689:703	polysaccharides	689:703	polysaccharides from Chao Massa Medicata Fermentata	689:739	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	21	contain	had	741:743	arg2	proportion					811:820	a lower proportion	803:820	a lower proportion of side chains	803:835	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	21	contain	had	741:743	arg2	weight					763:768	a lower molecular weight	745:768	a lower molecular weight	745:768	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	5	22	theme	inhibition	948:957	arg1	activity					959:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	23	from	activity	993:1000	arg1	Fermentata					1050:1059	Fermentata	1050:1059	Fermentata	1050:1059	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	4	24	theme	lower	805:809	arg1	proportion					811:820	a lower proportion	803:820	a lower proportion of side chains	803:835	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	1	25	theme	medicinal	216:224	arg1	slices					226:231	medicinal slices	216:231	medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata)	216:329	Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata).
36570240	2	26	theme	glycosidic	466:475	arg1	linkage					477:483	glycosidic linkage	466:483	glycosidic linkage	466:483	The physicochemical properties of these polysaccharides were studied, including the molecular weight, monosaccharide composition, and glycosidic linkage.
36570240	0	27	from	Fermentata	94:103	arg1	Properties					44:53	Physicochemical and Bioactive Properties	14:53	Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products	14:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	0	27	from	Fermentata	94:103	arg1	Polysaccharides					58:72	Polysaccharides	58:72	Polysaccharides from Massa Medicata Fermentata and Its Processed Products	58:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	1	28	theme	slices	226:231	arg1	types					207:211	different types	197:211	different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata)	197:329	Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata).
36570240	0	29	theme	Properties	44:53	arg1	Comparison					0:9	Comparison	0:9	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.	0:131	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	6	30	theme	polysaccharides	1160:1174	arg1	properties					1142:1151	the physicochemical properties	1122:1151	the physicochemical properties of the polysaccharides	1122:1174	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	0	31	theme	Bioactive	34:42	arg1	Properties					44:53	Physicochemical and Bioactive Properties	14:53	Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products	14:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	2	32	theme	monosaccharide	434:447	arg1	composition					449:459	monosaccharide composition	434:459	monosaccharide composition	434:459	The physicochemical properties of these polysaccharides were studied, including the molecular weight, monosaccharide composition, and glycosidic linkage.
36570240	0	33	theme	Polysaccharides	58:72	arg1	Properties					44:53	Physicochemical and Bioactive Properties	14:53	Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products	14:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	6	34	theme	physicochemical	1126:1140	arg1	properties					1142:1151	the physicochemical properties	1122:1151	the physicochemical properties of the polysaccharides	1122:1174	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	1	35	dep	Fermentata	251:260	arg1	Fermentata					319:328	Fermentata	319:328	Fermentata	319:328	Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata).
36570240	1	35	dep	Fermentata	251:260	arg1	Fermentata					284:293	Fermentata	284:293	Fermentata	284:293	Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata).
36570240	6	36	theme	inhibition	1217:1226	arg1	activity					1228:1235	reduced enzyme inhibition activity	1202:1235	reduced enzyme inhibition activity	1202:1235	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	2	37	theme	polysaccharides	372:386	arg1	properties					352:361	The physicochemical properties	332:361	The physicochemical properties of these polysaccharides	332:386	The physicochemical properties of these polysaccharides were studied, including the molecular weight, monosaccharide composition, and glycosidic linkage.
36570240	0	38	from	Products	123:130	arg1	Properties					44:53	Physicochemical and Bioactive Properties	14:53	Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products	14:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	0	38	from	Products	123:130	arg1	Polysaccharides					58:72	Polysaccharides	58:72	Polysaccharides from Massa Medicata Fermentata and Its Processed Products	58:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	6	39	from	increase	1244:1251	arg1	activity					1268:1275	antioxidant activity	1256:1275	antioxidant activity	1256:1275	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	4	40	theme	higher	771:776	arg1	content					790:796	higher uronic acid content	771:796	higher uronic acid content	771:796	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	6	41	theme	antioxidant	1256:1266	arg1	activity					1268:1275	antioxidant activity	1256:1275	antioxidant activity	1256:1275	These results indicated that stir-frying changes the physicochemical properties of the polysaccharides significantly, leading to reduced enzyme inhibition activity and an increase in antioxidant activity.
36570240	5	42	theme	antioxidant	981:991	arg1	activity					993:1000	the antioxidant activity	977:1000	the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata	977:1059	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	42	theme	antioxidant	981:991	arg1	higher					1065:1070	higher	1065:1070	higher	1065:1070	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	3	43	theme	α-amylase	519:527	arg1	inhibition					496:505	inhibition	496:505	inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity	496:600	Moreover, inhibition of trypsin, α-amylase, and α-glucosidase by the polysaccharides and their antioxidant activity were investigated.
36570240	1	44	theme	Fermentata	251:260	arg1	slices					226:231	medicinal slices	216:231	medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata)	216:329	Two polysaccharides were separately extracted and purified from different types of medicinal slices of Massa Medicata Fermentata (Sheng Massa Medicata Fermentata and Chao Massa Medicata Fermentata).
36570240	5	45	theme	α-amylase	919:927	arg1	activity					959:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	5	46	theme	stronger	901:908	arg1	activity					959:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	stronger trypsin, α-amylase, and α-glucosidase inhibition activity	901:966	Polysaccharides from Sheng Massa Medicata Fermentata displayed stronger trypsin, α-amylase, and α-glucosidase inhibition activity, whereas the antioxidant activity of the polysaccharides from Chao Massa Medicata Fermentata was higher.
36570240	4	47	theme	molecular	753:761	arg1	weight					763:768	a lower molecular weight	745:768	a lower molecular weight	745:768	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	4	48	from	Fermentata	677:686	arg1	polysaccharides					635:649	polysaccharides	635:649	polysaccharides from Sheng Massa Medicata Fermentata	635:686	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	0	49	theme	Physicochemical	14:28	arg1	Properties					44:53	Physicochemical and Bioactive Properties	14:53	Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products	14:130	Comparison of Physicochemical and Bioactive Properties of Polysaccharides from Massa Medicata Fermentata and Its Processed Products.
36570240	4	50	theme	lower	747:751	arg1	weight					763:768	a lower molecular weight	745:768	a lower molecular weight	745:768	Compared with polysaccharides from Sheng Massa Medicata Fermentata, polysaccharides from Chao Massa Medicata Fermentata had a lower molecular weight, higher uronic acid content, and a lower proportion of side chains.
36570240	7	51	theme	Fermentata	1357:1366	arg1	application					1327:1337	the selective application	1313:1337	the selective application of Massa Medicata Fermentata	1313:1366	This research provides a guide for the selective application of Massa Medicata Fermentata.
36570240	7	52	theme	selective	1317:1325	arg1	application					1327:1337	the selective application	1313:1337	the selective application of Massa Medicata Fermentata	1313:1366	This research provides a guide for the selective application of Massa Medicata Fermentata.
36515414	4	0	theme	bacteriotherapy	889:903	arg1	T0					853:854	T0	853:854	T0	853:854	METHODS Long-term virologically suppressed PLWH underwent blood, colonic and fecal sampling before (T0) and after 6 months (T6) of oral bacteriotherapy.
36515414	4	0	theme	bacteriotherapy	889:903	arg1	T6					877:878	T6	877:878	T6	877:878	METHODS Long-term virologically suppressed PLWH underwent blood, colonic and fecal sampling before (T0) and after 6 months (T6) of oral bacteriotherapy.
36515414	4	0	theme	bacteriotherapy	889:903	arg1	months					869:874	6 months	867:874	6 months (T6) of oral bacteriotherapy	867:903	METHODS Long-term virologically suppressed PLWH underwent blood, colonic and fecal sampling before (T0) and after 6 months (T6) of oral bacteriotherapy.
36515414	13	1	theme	therapy	2357:2363	arg1	PLWH					2373:2376	antiretroviral therapy treated PLWH	2342:2376	antiretroviral therapy treated PLWH	2342:2376	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	7	2	theme	physiological	1136:1148	arg1	functions					1150:1158	physiological functions	1136:1158	physiological functions	1136:1158	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	8	3	theme	flora	1256:1260	arg1	composition					1262:1272	gut microbial flora composition	1242:1272	gut microbial flora composition	1242:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	10	4	theme	oral	1779:1782	arg1	p<0.05					1801:1806	p<0.05	1801:1806	p<0.05	1801:1806	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	10	4	theme	oral	1779:1782	arg1	bacteriotherapy					1784:1798	oral bacteriotherapy	1779:1798	oral bacteriotherapy (p<0.05)	1779:1807	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	13	5	theme	gut	2328:2330	arg1	mucosa					2332:2337	the gut mucosa	2324:2337	the gut mucosa	2324:2337	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	1	6	theme	intestinal	177:186	arg1	barrier					196:202	the intestinal mucosal barrier	173:202	the intestinal mucosal barrier	173:202	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	1	7	theme	barrier	196:202	arg1	damage					163:168	BACKGROUND Functional and structural damage	126:168	BACKGROUND Functional and structural damage of the intestinal mucosal barrier	126:202	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	12	8	located	observed	2007:2014	arg1	tracts					2035:2040	PLWH intestinal tracts	2019:2040	PLWH intestinal tracts	2019:2040	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	12	8	located	observed	2007:2014	arg2	concentration					1988:2000	increased occludin and zonulin concentration	1957:2000	increased occludin and zonulin concentration	1957:2000	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	11	9	theme	structure	1893:1901	arg1	number					1852:1857	number	1852:1857	number	1852:1857	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	9	theme	structure	1893:1901	arg1	size					1833:1836	Increased mitochondria size	1810:1836	Increased mitochondria size (p=0.005)	1810:1846	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	9	theme	structure	1893:1901	arg1	p=0.005					1839:1845	p=0.005	1839:1845	p=0.005	1839:1845	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	9	theme	structure	1893:1901	arg1	p=0.008					1860:1866	p=0.008	1860:1866	p=0.008	1860:1866	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	9	theme	structure	1893:1901	arg1	amelioration					1873:1884	amelioration	1873:1884	amelioration of AJC structure (p<0.0001)	1873:1912	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	7	10	theme	complex	1107:1113	arg1	morphology					1121:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology	1051:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology	1051:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	10	11	located	observed	1748:1755	arg1	PLWH					1764:1767	all PLWH	1760:1767	all PLWH receiving oral bacteriotherapy (p<0.05)	1760:1807	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	10	11	located	observed	1748:1755	arg2	Restoration					1661:1671	Restoration	1661:1671	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index)	1661:1742	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	9	12	from	number	1559:1564	arg1	structures					1624:1633	AJC structures	1620:1633	AJC structures (p<0.0001)	1620:1644	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	12	from	number	1559:1564	arg1	p<0.0001					1636:1643	p<0.0001	1636:1643	p<0.0001	1636:1643	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	13	theme	mitochondria	1579:1590	arg1	p=0.005					1546:1552	p=0.005	1546:1552	p=0.005	1546:1552	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	13	theme	mitochondria	1579:1590	arg1	number					1559:1564	number	1559:1564	number (p=0.008)	1559:1574	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	13	theme	mitochondria	1579:1590	arg1	size					1540:1543	size	1540:1543	size (p=0.005)	1540:1553	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	13	theme	mitochondria	1579:1590	arg1	p=0.008					1567:1573	p=0.008	1567:1573	p=0.008	1567:1573	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	8	14	theme	occludin	1291:1298	arg1	levels					1281:1286	lower levels	1275:1286	lower levels of occludin (p=0.002) and zonulin (p=0.01)	1275:1329	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	14	theme	occludin	1291:1298	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	7	15	theme	apical	1089:1094	arg1	AJC					1116:1118	AJC	1116:1118	AJC	1116:1118	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	7	15	theme	apical	1089:1094	arg1	complex					1107:1113	apical junctional complex	1089:1113	apical junctional complex (AJC)	1089:1119	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	11	16	theme	Increased	1810:1818	arg1	size					1833:1836	Increased mitochondria size	1810:1836	Increased mitochondria size (p=0.005)	1810:1846	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	16	theme	Increased	1810:1818	arg1	p=0.008					1860:1866	p=0.008	1860:1866	p=0.008	1860:1866	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	16	theme	Increased	1810:1818	arg1	p=0.005					1839:1845	p=0.005	1839:1845	p=0.005	1839:1845	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	8	17	theme	gut	1242:1244	arg1	composition					1262:1272	gut microbial flora composition	1242:1272	gut microbial flora composition	1242:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	14	18	dep	Rome	2482:2485	arg1	Medi					2522:2525	Medi	2522:2525	Medi	2522:2525	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	14	18	dep	Rome	2482:2485	arg1	Number					2605:2610	Protocol Number	2596:2610	Protocol Number	2596:2610	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	14	18	dep	Rome	2482:2485	arg1	N.					2537:2538	N.	2537:2538	N.	2537:2538	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	14	18	dep	Rome	2482:2485	arg1	Progetti					2513:2520	Progetti	2513:2520	Progetti	2513:2520	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	7	19	theme	mitochondria	1072:1083	arg1	morphology					1121:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology	1051:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology	1051:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	4	20	theme	suppressed	785:794	arg1	PLWH					796:799	virologically suppressed PLWH	771:799	virologically suppressed PLWH	771:799	METHODS Long-term virologically suppressed PLWH underwent blood, colonic and fecal sampling before (T0) and after 6 months (T6) of oral bacteriotherapy.
36515414	7	21	theme	machinery	1061:1069	arg1	morphology					1121:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology	1051:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology	1051:1130	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	1	22	theme	Functional	137:146	arg1	damage					163:168	BACKGROUND Functional and structural damage	126:168	BACKGROUND Functional and structural damage of the intestinal mucosal barrier	126:202	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	9	23	from	amelioration	1604:1615	arg1	structures					1624:1633	AJC structures	1620:1633	AJC structures (p<0.0001)	1620:1644	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	23	from	amelioration	1604:1615	arg1	p<0.0001					1636:1643	p<0.0001	1636:1643	p<0.0001	1636:1643	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	3	24	theme	microbiota	666:675	arg1	modulation					648:657	the modulation	644:657	the modulation of gut microbiota	644:675	This study aims to better understand whether the modulation of gut microbiota promotes an intestinal immune restoration in people living with HIV (PLWH).
36515414	5	25	theme	gender-matched	915:928	arg1	UC					951:952	UC	951:952	UC	951:952	Age- and gender-matched uninfected controls (UC) were also included.
36515414	5	25	theme	gender-matched	915:928	arg1	controls					941:948	Age- and gender-matched uninfected controls	906:948	Age- and gender-matched uninfected controls (UC)	906:953	Age- and gender-matched uninfected controls (UC) were also included.
36515414	2	26	theme	derived	538:544	arg1	components					546:555	microbial derived components	528:555	microbial derived components	528:555	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	13	27	theme	structural	2286:2295	arg1	recovery					2312:2319	the structural and functional recovery	2282:2319	the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH	2282:2376	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	0	28	theme	microbiota	76:85	arg1	alterations					87:97	microbiota alterations	76:97	microbiota alterations	76:97	The crosstalk between gut barrier impairment, mitochondrial dysfunction and microbiota alterations in people living with HIV.
36515414	2	29	theme	disease	578:584	arg1	progression					586:596	HIV-1 disease progression	572:596	HIV-1 disease progression	572:596	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	8	30	theme	higher	1332:1337	arg1	p=0.002					1357:1363	p=0.002	1357:1363	p=0.002	1357:1363	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	30	theme	higher	1332:1337	arg1	levels					1349:1354	higher Claudin-2 levels	1332:1354	higher Claudin-2 levels (p=0.002)	1332:1364	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	30	theme	higher	1332:1337	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	9	31	from	increase	1528:1535	arg1	p=0.005					1546:1552	p=0.005	1546:1552	p=0.005	1546:1552	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	31	from	increase	1528:1535	arg1	number					1559:1564	number	1559:1564	number (p=0.008)	1559:1574	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	31	from	increase	1528:1535	arg1	size					1540:1543	size	1540:1543	size (p=0.005)	1540:1553	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	31	from	increase	1528:1535	arg1	p=0.008					1567:1573	p=0.008	1567:1573	p=0.008	1567:1573	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	8	32	theme	LPS	1467:1469	arg1	levels					1457:1462	increased levels	1447:1462	increased levels of LPS (p=0.018) and cCK18 (p=0.011)	1447:1499	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	32	theme	LPS	1467:1469	arg1	p=0.002					1403:1409	p=0.002	1403:1409	p=0.002	1403:1409	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	32	theme	LPS	1467:1469	arg1	diameter					1416:1423	diameter (p=0.002)	1416:1433	diameter (p=0.002)	1416:1433	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	32	theme	LPS	1467:1469	arg1	number					1395:1400	mitochondria number	1382:1400	mitochondria number (p=0.002)	1382:1410	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	12	33	theme	Claudin-2	2066:2074	arg1	levels					2056:2061	decreased levels	2046:2061	decreased levels of Claudin-2, LPS and cCK18	2046:2089	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	0	34	theme	impairment	34:43	arg1	dysfunction					60:70	gut barrier impairment, mitochondrial dysfunction	22:70	dysfunction	60:70	The crosstalk between gut barrier impairment, mitochondrial dysfunction and microbiota alterations in people living with HIV.
36515414	13	35	theme	flora	2264:2268	arg1	balance					2242:2248	the balance	2238:2248	the balance of intestinal flora	2238:2268	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	12	36	theme	intestinal	2024:2033	arg1	tracts					2035:2040	PLWH intestinal tracts	2019:2040	PLWH intestinal tracts	2019:2040	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	11	37	theme	mitochondria	1820:1831	arg1	size					1833:1836	Increased mitochondria size	1810:1836	Increased mitochondria size (p=0.005)	1810:1846	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	37	theme	mitochondria	1820:1831	arg1	p=0.008					1860:1866	p=0.008	1860:1866	p=0.008	1860:1866	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	37	theme	mitochondria	1820:1831	arg1	p=0.005					1839:1845	p=0.005	1839:1845	p=0.005	1839:1845	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	2	38	theme	phagocytic	462:471	arg1	function					473:480	intestinal macrophage phagocytic function	440:480	intestinal macrophage phagocytic function	440:480	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	4	39	dep	METHODS	753:759	arg1	Long-term					761:769	Long-term	761:769	Long-term	761:769	METHODS Long-term virologically suppressed PLWH underwent blood, colonic and fecal sampling before (T0) and after 6 months (T6) of oral bacteriotherapy.
36515414	10	40	theme	biodiversity	1715:1726	arg1	Restoration					1661:1671	Restoration	1661:1671	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index)	1661:1742	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	2	41	theme	intestinal	440:449	arg1	function					473:480	intestinal macrophage phagocytic function	440:480	intestinal macrophage phagocytic function	440:480	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	0	42	theme	barrier	26:32	arg1	dysfunction					60:70	gut barrier impairment, mitochondrial dysfunction	22:70	dysfunction	60:70	The crosstalk between gut barrier impairment, mitochondrial dysfunction and microbiota alterations in people living with HIV.
36515414	1	43	theme	HIV-1	321:325	arg1	activation					353:362	HIV-1 associated chronic immune activation	321:362	HIV-1 associated chronic immune activation	321:362	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	2	44	theme	components	546:555	arg1	accumulation					512:523	extracellular accumulation	498:523	extracellular accumulation of microbial derived components	498:555	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	13	45	theme	INTERPRETATION	2172:2185	arg1	supplementation					2208:2222	INTERPRETATION Oral bacteriotherapy supplementation	2172:2222	INTERPRETATION Oral bacteriotherapy supplementation	2172:2222	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	2	46	theme	extracellular	498:510	arg1	accumulation					512:523	extracellular accumulation	498:523	extracellular accumulation of microbial derived components	498:555	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	3	47	theme	immune	700:705	arg1	restoration					707:717	an intestinal immune restoration	686:717	an intestinal immune restoration in people living with HIV (PLWH)	686:750	This study aims to better understand whether the modulation of gut microbiota promotes an intestinal immune restoration in people living with HIV (PLWH).
36515414	8	48	theme	mitochondria	1382:1393	arg1	p=0.002					1403:1409	p=0.002	1403:1409	p=0.002	1403:1409	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	48	theme	mitochondria	1382:1393	arg1	number					1395:1400	mitochondria number	1382:1400	mitochondria number (p=0.002)	1382:1410	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	2	49	theme	microbial	370:378	arg1	translocation					380:392	This microbial translocation	365:392	This microbial translocation	365:392	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	1	50	theme	chronic	338:344	arg1	activation					353:362	HIV-1 associated chronic immune activation	321:362	HIV-1 associated chronic immune activation	321:362	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	9	51	from	structures	1624:1633	arg1	p=0.005					1546:1552	p=0.005	1546:1552	p=0.005	1546:1552	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	51	from	structures	1624:1633	arg1	number					1559:1564	number	1559:1564	number (p=0.008)	1559:1574	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	51	from	structures	1624:1633	arg1	size					1540:1543	size	1540:1543	size (p=0.005)	1540:1553	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	51	from	structures	1624:1633	arg1	p=0.008					1567:1573	p=0.008	1567:1573	p=0.008	1567:1573	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	13	52	theme	bacteriotherapy	2192:2206	arg1	supplementation					2208:2222	INTERPRETATION Oral bacteriotherapy supplementation	2172:2222	INTERPRETATION Oral bacteriotherapy supplementation	2172:2222	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	10	53	theme	richness	1686:1693	arg1	Restoration					1661:1671	Restoration	1661:1671	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index)	1661:1742	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	12	54	theme	occludin	1967:1974	arg1	concentration					1988:2000	increased occludin and zonulin concentration	1957:2000	increased occludin and zonulin concentration	1957:2000	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	1	55	theme	structural	152:161	arg1	damage					163:168	BACKGROUND Functional and structural damage	126:168	BACKGROUND Functional and structural damage of the intestinal mucosal barrier	126:202	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	10	56	theme	Shannon	1729:1735	arg1	biodiversity					1715:1726	biodiversity	1715:1726	biodiversity (Shannon index)	1715:1742	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	10	56	theme	Shannon	1729:1735	arg1	index					1737:1741	Shannon index	1729:1741	Shannon index	1729:1741	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	12	57	theme	zonulin	1980:1986	arg1	concentration					1988:2000	increased occludin and zonulin concentration	1957:2000	increased occludin and zonulin concentration	1957:2000	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	14	58	dep	Medi	2522:2525	arg1	Progetti					2575:2582	Progetti	2575:2582	Progetti	2575:2582	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	14	58	dep	Medi	2522:2525	arg1	RM120172B7C4C0E0					2613:2628	RM120172B7C4C0E0	2613:2628	RM120172B7C4C0E0	2613:2628	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	14	58	dep	Medi	2522:2525	arg1	Medi					2584:2587	Medi	2584:2587	Medi	2584:2587	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	1	59	theme	microbial	253:261	arg1	products					263:270	gut microbial products	249:270	gut microbial products	249:270	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	13	60	theme	antiretroviral	2342:2355	arg1	therapy					2357:2363	antiretroviral therapy	2342:2363	antiretroviral therapy treated PLWH	2342:2376	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	8	61	from	PLWH	1207:1210	arg1	FINDINGS					1191:1198	FINDINGS	1191:1198	FINDINGS At T0, PLWH	1191:1210	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	4	62	theme	oral	884:887	arg1	bacteriotherapy					889:903	oral bacteriotherapy	884:903	oral bacteriotherapy	884:903	METHODS Long-term virologically suppressed PLWH underwent blood, colonic and fecal sampling before (T0) and after 6 months (T6) of oral bacteriotherapy.
36515414	13	63	theme	treated	2365:2371	arg1	PLWH					2373:2376	antiretroviral therapy treated PLWH	2342:2376	antiretroviral therapy treated PLWH	2342:2376	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	3	64	from	restoration	707:717	arg1	people					722:727	people	722:727	people living with HIV (PLWH)	722:750	This study aims to better understand whether the modulation of gut microbiota promotes an intestinal immune restoration in people living with HIV (PLWH).
36515414	11	65	located	found	1919:1923	arg2	amelioration					1873:1884	amelioration	1873:1884	amelioration of AJC structure (p<0.0001)	1873:1912	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	65	located	found	1919:1923	arg1	T6					1928:1929	T6	1928:1929	T6	1928:1929	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	65	located	found	1919:1923	arg2	size					1833:1836	Increased mitochondria size	1810:1836	Increased mitochondria size (p=0.005)	1810:1846	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	65	located	found	1919:1923	arg2	number					1852:1857	number	1852:1857	number	1852:1857	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	65	located	found	1919:1923	arg2	p=0.005					1839:1845	p=0.005	1839:1845	p=0.005	1839:1845	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	65	located	found	1919:1923	arg2	p=0.008					1860:1866	p=0.008	1860:1866	p=0.008	1860:1866	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	13	66	from	recovery	2312:2319	arg1	PLWH					2373:2376	antiretroviral therapy treated PLWH	2342:2376	antiretroviral therapy treated PLWH	2342:2376	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	8	67	theme	microbial	1246:1254	arg1	composition					1262:1272	gut microbial flora composition	1242:1272	gut microbial flora composition	1242:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	13	68	theme	mucosa	2332:2337	arg1	recovery					2312:2319	the structural and functional recovery	2282:2319	the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH	2282:2376	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	1	69	theme	mucosal	188:194	arg1	barrier					196:202	the intestinal mucosal barrier	173:202	the intestinal mucosal barrier	173:202	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	12	70	theme	oral	2108:2111	arg1	bacteriotherapy					2113:2127	oral bacteriotherapy	2108:2127	oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures)	2108:2169	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	8	71	theme	composition	1262:1272	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	71	theme	composition	1262:1272	arg1	levels					1349:1354	higher Claudin-2 levels	1332:1354	higher Claudin-2 levels (p=0.002)	1332:1364	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	71	theme	composition	1262:1272	arg1	reduction					1369:1377	a reduction	1367:1377	a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011),	1367:1500	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	71	theme	composition	1262:1272	arg1	levels					1281:1286	lower levels	1275:1286	lower levels of occludin (p=0.002) and zonulin (p=0.01)	1275:1329	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	7	72	theme	junctional	1096:1105	arg1	AJC					1116:1118	AJC	1116:1118	AJC	1116:1118	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	7	72	theme	junctional	1096:1105	arg1	complex					1107:1113	apical junctional complex	1089:1113	apical junctional complex (AJC)	1089:1119	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	6	73	theme	gene	984:987	arg1	sequencing					989:998	16S rRNA gene sequencing	975:998	16S rRNA gene sequencing	975:998	16S rRNA gene sequencing was applied to all participants' fecal microbiota.
36515414	8	74	theme	diameter	1416:1423	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	74	theme	diameter	1416:1423	arg1	reduction					1369:1377	a reduction	1367:1377	a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011),	1367:1500	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	14	75	theme	Protocol	2596:2603	arg1	Medi					2522:2525	Medi	2522:2525	Medi	2522:2525	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	14	75	theme	Protocol	2596:2603	arg1	Number					2605:2610	Protocol Number	2596:2610	Protocol Number	2596:2610	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	8	76	theme	lower	1275:1279	arg1	levels					1281:1286	lower levels	1275:1286	lower levels of occludin (p=0.002) and zonulin (p=0.01)	1275:1329	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	76	theme	lower	1275:1279	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	10	77	theme	bacterial	1676:1684	arg1	richness					1686:1693	bacterial richness	1676:1693	bacterial richness (Simpson index)	1676:1709	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	10	77	theme	bacterial	1676:1684	arg1	index					1704:1708	index	1704:1708	index	1704:1708	Restoration of bacterial richness (Simpson index) and biodiversity (Shannon index) was observed in all PLWH receiving oral bacteriotherapy (p<0.05).
36515414	5	78	theme	Age-	906:909	arg1	UC					951:952	UC	951:952	UC	951:952	Age- and gender-matched uninfected controls (UC) were also included.
36515414	5	78	theme	Age-	906:909	arg1	controls					941:948	Age- and gender-matched uninfected controls	906:948	Age- and gender-matched uninfected controls (UC)	906:953	Age- and gender-matched uninfected controls (UC) were also included.
36515414	8	79	theme	different	1221:1229	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	79	theme	different	1221:1229	arg1	levels					1349:1354	higher Claudin-2 levels	1332:1354	higher Claudin-2 levels (p=0.002)	1332:1364	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	79	theme	different	1221:1229	arg1	reduction					1369:1377	a reduction	1367:1377	a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011),	1367:1500	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	79	theme	different	1221:1229	arg1	levels					1281:1286	lower levels	1275:1286	lower levels of occludin (p=0.002) and zonulin (p=0.01)	1275:1329	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	6	80	theme	16S	975:977	arg1	sequencing					989:998	16S rRNA gene sequencing	975:998	16S rRNA gene sequencing	975:998	16S rRNA gene sequencing was applied to all participants' fecal microbiota.
36515414	11	81	theme	AJC	1889:1891	arg1	structure					1893:1901	AJC structure	1889:1901	AJC structure (p<0.0001)	1889:1912	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	11	81	theme	AJC	1889:1891	arg1	p<0.0001					1904:1911	p<0.0001	1904:1911	p<0.0001	1904:1911	Increased mitochondria size (p=0.005) and number (p=0.008) and amelioration of AJC structure (p<0.0001) were found at T6 compared to T0.
36515414	2	82	link	derived	538:544	arg1	components					546:555	microbial derived components	528:555	microbial derived components	528:555	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	9	83	from	size	1540:1543	arg1	structures					1624:1633	AJC structures	1620:1633	AJC structures (p<0.0001)	1620:1644	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	83	from	size	1540:1543	arg1	p<0.0001					1636:1643	p<0.0001	1636:1643	p<0.0001	1636:1643	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	7	84	theme	Apoptosis	1051:1059	arg1	machinery					1061:1069	Apoptosis machinery	1051:1069	Apoptosis machinery	1051:1069	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	1	85	theme	BACKGROUND	126:135	arg1	damage					163:168	BACKGROUND Functional and structural damage	126:168	BACKGROUND Functional and structural damage of the intestinal mucosal barrier	126:202	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	14	86	theme	research	2415:2422	arg1	grants					2424:2429	research grants	2415:2429	research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0)	2415:2629	FUNDING This study was supported by research grants to Gabriella d'Ettorre from Sapienza University of Rome (Ricerca Ateneo Sapienza, Progetti Medi, 2018 DDA N. 801/2019; Ricerca Ateneo Sapienza, Progetti Medi, 2020, Protocol Number: RM120172B7C4C0E0).
36515414	9	87	theme	amelioration	1604:1615	arg1	p=0.005					1546:1552	p=0.005	1546:1552	p=0.005	1546:1552	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	87	theme	amelioration	1604:1615	arg1	number					1559:1564	number	1559:1564	number (p=0.008)	1559:1574	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	87	theme	amelioration	1604:1615	arg1	size					1540:1543	size	1540:1543	size (p=0.005)	1540:1553	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	87	theme	amelioration	1604:1615	arg1	p=0.008					1567:1573	p=0.008	1567:1573	p=0.008	1567:1573	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	3	88	theme	gut	662:664	arg1	microbiota					666:675	gut microbiota	662:675	gut microbiota	662:675	This study aims to better understand whether the modulation of gut microbiota promotes an intestinal immune restoration in people living with HIV (PLWH).
36515414	6	89	theme	fecal	1033:1037	arg1	microbiota					1039:1048	all participants' fecal microbiota	1015:1048	all participants' fecal microbiota	1015:1048	16S rRNA gene sequencing was applied to all participants' fecal microbiota.
36515414	9	90	theme	AJC	1620:1622	arg1	structures					1624:1633	AJC structures	1620:1633	AJC structures (p<0.0001)	1620:1644	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	90	theme	AJC	1620:1622	arg1	p<0.0001					1636:1643	p<0.0001	1636:1643	p<0.0001	1636:1643	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	8	91	theme	increased	1447:1455	arg1	levels					1457:1462	increased levels	1447:1462	increased levels of LPS (p=0.018) and cCK18 (p=0.011)	1447:1499	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	92	theme	zonulin	1314:1320	arg1	levels					1281:1286	lower levels	1275:1286	lower levels of occludin (p=0.002) and zonulin (p=0.01)	1275:1329	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	92	theme	zonulin	1314:1320	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	5	93	theme	uninfected	930:939	arg1	UC					951:952	UC	951:952	UC	951:952	Age- and gender-matched uninfected controls (UC) were also included.
36515414	5	93	theme	uninfected	930:939	arg1	controls					941:948	Age- and gender-matched uninfected controls	906:948	Age- and gender-matched uninfected controls (UC)	906:953	Age- and gender-matched uninfected controls (UC) were also included.
36515414	9	94	from	mitochondria	1579:1590	arg1	structures					1624:1633	AJC structures	1620:1633	AJC structures (p<0.0001)	1620:1644	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	94	from	mitochondria	1579:1590	arg1	p<0.0001					1636:1643	p<0.0001	1636:1643	p<0.0001	1636:1643	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	2	95	theme	microbial	528:536	arg1	components					546:555	microbial derived components	528:555	microbial derived components	528:555	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	0	96	from	crosstalk	4:12	arg1	people					102:107	people	102:107	people living with HIV	102:123	The crosstalk between gut barrier impairment, mitochondrial dysfunction and microbiota alterations in people living with HIV.
36515414	12	97	theme	increased	1957:1965	arg1	concentration					1988:2000	increased occludin and zonulin concentration	1957:2000	increased occludin and zonulin concentration	1957:2000	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	2	98	theme	HIV-1	572:576	arg1	progression					586:596	HIV-1 disease progression	572:596	HIV-1 disease progression	572:596	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	12	99	theme	LPS	2077:2079	arg1	levels					2056:2061	decreased levels	2046:2061	decreased levels of Claudin-2, LPS and cCK18	2046:2089	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	12	100	theme	decreased	2046:2054	arg1	levels					2056:2061	decreased levels	2046:2061	decreased levels of Claudin-2, LPS and cCK18	2046:2089	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	13	101	theme	intestinal	2253:2262	arg1	flora					2264:2268	intestinal flora	2253:2268	intestinal flora	2253:2268	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	12	102	theme	cCK18	2085:2089	arg1	levels					2056:2061	decreased levels	2046:2061	decreased levels of Claudin-2, LPS and cCK18	2046:2089	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	13	103	theme	functional	2301:2310	arg1	recovery					2312:2319	the structural and functional recovery	2282:2319	the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH	2282:2376	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	4	104	theme	fecal	830:834	arg1	sampling					836:843	fecal sampling	830:843	fecal sampling	830:843	METHODS Long-term virologically suppressed PLWH underwent blood, colonic and fecal sampling before (T0) and after 6 months (T6) of oral bacteriotherapy.
36515414	2	105	theme	progressive	414:424	arg1	exhaustion					426:435	a progressive exhaustion	412:435	a progressive exhaustion	412:435	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	6	106	theme	rRNA	979:982	arg1	sequencing					989:998	16S rRNA gene sequencing	975:998	16S rRNA gene sequencing	975:998	16S rRNA gene sequencing was applied to all participants' fecal microbiota.
36515414	8	107	theme	cCK18	1485:1489	arg1	levels					1457:1462	increased levels	1447:1462	increased levels of LPS (p=0.018) and cCK18 (p=0.011)	1447:1499	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	107	theme	cCK18	1485:1489	arg1	p=0.002					1403:1409	p=0.002	1403:1409	p=0.002	1403:1409	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	107	theme	cCK18	1485:1489	arg1	diameter					1416:1423	diameter (p=0.002)	1416:1433	diameter (p=0.002)	1416:1433	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	107	theme	cCK18	1485:1489	arg1	number					1395:1400	mitochondria number	1382:1400	mitochondria number (p=0.002)	1382:1410	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	12	108	theme	PLWH	2019:2022	arg1	tracts					2035:2040	PLWH intestinal tracts	2019:2040	PLWH intestinal tracts	2019:2040	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	0	109	theme	gut	22:24	arg1	dysfunction					60:70	gut barrier impairment, mitochondrial dysfunction	22:70	dysfunction	60:70	The crosstalk between gut barrier impairment, mitochondrial dysfunction and microbiota alterations in people living with HIV.
36515414	9	110	located	observed	1651:1658	arg2	increase					1528:1535	an increase	1525:1535	an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001)	1525:1644	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	9	110	located	observed	1651:1658	arg1	T6					1521:1522	T6	1521:1522	T6	1521:1522	At T6, an increase in size (p=0.005) and number (p=0.008) of mitochondria, as well as amelioration in AJC structures (p<0.0001) were observed.
36515414	2	111	theme	function	473:480	arg1	exhaustion					426:435	a progressive exhaustion	412:435	a progressive exhaustion	412:435	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	2	112	theme	macrophage	451:460	arg1	function					473:480	intestinal macrophage phagocytic function	440:480	intestinal macrophage phagocytic function	440:480	This microbial translocation is largely due to a progressive exhaustion of intestinal macrophage phagocytic function, which leads to extracellular accumulation of microbial derived components and results in HIV-1 disease progression.
36515414	7	113	theme	gut	1177:1179	arg1	biopsies					1181:1188	gut biopsies	1177:1188	gut biopsies	1177:1188	Apoptosis machinery, mitochondria and apical junctional complex (AJC) morphology and physiological functions were analyzed in gut biopsies.
36515414	3	114	theme	intestinal	689:698	arg1	restoration					707:717	an intestinal immune restoration	686:717	an intestinal immune restoration in people living with HIV (PLWH)	686:750	This study aims to better understand whether the modulation of gut microbiota promotes an intestinal immune restoration in people living with HIV (PLWH).
36515414	0	115	theme	mitochondrial	46:58	arg1	dysfunction					60:70	gut barrier impairment, mitochondrial dysfunction	22:70	dysfunction	60:70	The crosstalk between gut barrier impairment, mitochondrial dysfunction and microbiota alterations in people living with HIV.
36515414	1	116	theme	associated	327:336	arg1	activation					353:362	HIV-1 associated chronic immune activation	321:362	HIV-1 associated chronic immune activation	321:362	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	13	117	theme	Oral	2187:2190	arg1	supplementation					2208:2222	INTERPRETATION Oral bacteriotherapy supplementation	2172:2222	INTERPRETATION Oral bacteriotherapy supplementation	2172:2222	INTERPRETATION Oral bacteriotherapy supplementation might restore the balance of intestinal flora and support the structural and functional recovery of the gut mucosa in antiretroviral therapy treated PLWH.
36515414	8	118	dep	showed	1212:1217	arg1	compared					1502:1509	compared	1502:1509	showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC	1212:1515	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	1	119	theme	immune	346:351	arg1	activation					353:362	HIV-1 associated chronic immune activation	321:362	HIV-1 associated chronic immune activation	321:362	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	8	120	theme	Claudin-2	1339:1347	arg1	p=0.002					1357:1363	p=0.002	1357:1363	p=0.002	1357:1363	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	120	theme	Claudin-2	1339:1347	arg1	levels					1349:1354	higher Claudin-2 levels	1332:1354	higher Claudin-2 levels (p=0.002)	1332:1364	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	120	theme	Claudin-2	1339:1347	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	121	theme	number	1395:1400	arg1	pattern					1231:1237	a different pattern	1219:1237	a different pattern of gut microbial flora composition	1219:1272	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	8	121	theme	number	1395:1400	arg1	reduction					1369:1377	a reduction	1367:1377	a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011),	1367:1500	FINDINGS At T0, PLWH showed a different pattern of gut microbial flora composition, lower levels of occludin (p=0.002) and zonulin (p=0.01), higher Claudin-2 levels (p=0.002), a reduction of mitochondria number (p=0.002) and diameter (p=0.002), as well as increased levels of LPS (p=0.018) and cCK18 (p=0.011), compared to UC.
36515414	1	122	theme	gut	249:251	arg1	products					263:270	gut microbial products	249:270	gut microbial products	249:270	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
36515414	12	123	dep	bacteriotherapy	2113:2127	arg1	T0					2130:2131	T0	2130:2131	T0	2130:2131	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	12	123	dep	bacteriotherapy	2113:2127	arg1	p<0.05					2140:2145	p<0.05	2140:2145	p<0.05 for all these measures	2140:2168	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	12	123	dep	bacteriotherapy	2113:2127	arg1	T6					2136:2137	T6	2136:2137	T6	2136:2137	Moreover, increased occludin and zonulin concentration were observed in PLWH intestinal tracts and decreased levels of Claudin-2, LPS and cCK18 were found after oral bacteriotherapy (T0 vs T6, p<0.05 for all these measures).
36515414	1	124	theme	products	263:270	arg1	translocation					232:244	translocation	232:244	translocation of gut microbial products into the bloodstream	232:291	BACKGROUND Functional and structural damage of the intestinal mucosal barrier significantly contribute to translocation of gut microbial products into the bloodstream and are largely involved in HIV-1 associated chronic immune activation.
37016467	10	0	theme	O-glycan	1804:1811	arg1	degradation					1813:1823	mucin O-glycan degradation	1798:1823	mucin O-glycan degradation	1798:1823	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	14	1	theme	Pediococcus	2259:2269	arg1	pathway					2303:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	6	2	theme	digesta	1060:1066	arg1	samples					1068:1074	digesta samples	1060:1074	digesta samples	1060:1074	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	7	3	theme	metabolic	1280:1288	arg1	capacity					1290:1297	predicted metabolic capacity	1270:1297	predicted metabolic capacity	1270:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	7	3	theme	metabolic	1280:1288	arg1	RESULTS					1232:1238	RESULTS The microbial composition and predicted metabolic capacity	1232:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota	1232:1315	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	12	4	theme	gut	2094:2096	arg1	microbiota					2098:2107	gut microbiota	2094:2107	gut microbiota of fish	2094:2115	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	9	5	dep	yeasts	1595:1600	arg1	AWA					1611:1613	AWA	1611:1613	AWA	1611:1613	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	9	5	dep	yeasts	1595:1600	arg1	ACJ					1603:1605	ACJ	1603:1605	ACJ	1603:1605	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	9	5	dep	yeasts	1595:1600	arg1	yeasts					1595:1600	autolyzed yeasts	1585:1600	autolyzed yeasts (ACJ and AWA diets)	1585:1620	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	2	6	theme	inclusion	249:257	arg1	impact					233:238	the impact	229:238	the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients	229:332	However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited.
37016467	7	7	theme	microbial	1244:1252	arg1	composition					1254:1264	The microbial composition	1240:1264	The microbial composition	1240:1264	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	7	7	theme	microbial	1244:1252	arg1	RESULTS					1232:1238	RESULTS The microbial composition and predicted metabolic capacity	1232:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota	1232:1315	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	3	8	theme	-based	543:548	arg1	diet					550:553	soybean meal (SBM)-based diet	525:553	soybean meal (SBM)-based diet	525:553	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	9	9	theme	microbiota	1664:1673	arg1	diversity					1647:1655	diversity	1647:1655	diversity	1647:1655	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	9	9	theme	microbiota	1664:1673	arg1	richness					1634:1641	richness	1634:1641	richness	1634:1641	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	9	10	dep	richness	1634:1641	arg1	the					1630:1632	the	1630:1632	the	1630:1632	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	2	11	theme	intestinal	276:285	arg1	microbiota					287:296	intestinal microbiota	276:296	intestinal microbiota of fish fed plant-based ingredients	276:332	However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited.
37016467	1	12	theme	alternative	182:192	arg1	attention					169:177	attention	169:177	attention	169:177	BACKGROUND Yeasts are gaining attention as alternative ingredients in aquafeeds.
37016467	1	12	theme	alternative	182:192	arg1	ingredients					194:204	alternative ingredients	182:204	alternative ingredients in aquafeeds	182:217	BACKGROUND Yeasts are gaining attention as alternative ingredients in aquafeeds.
37016467	10	13	theme	ACJ	1715:1717	arg1	diet					1719:1722	ACJ diet	1715:1722	ACJ diet	1715:1722	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	0	14	theme	meal-based	109:118	arg1	diets					120:124	soybean meal-based diets	101:124	soybean meal-based diets	101:124	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	1	15	from	ingredients	194:204	arg1	aquafeeds					209:217	aquafeeds	209:217	aquafeeds	209:217	BACKGROUND Yeasts are gaining attention as alternative ingredients in aquafeeds.
37016467	10	16	theme	fish	1706:1709	arg1	microbiota					1692:1701	The gut microbiota	1684:1701	The gut microbiota of fish	1684:1709	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	8	17	theme	inactivated	1532:1542	arg1	yeasts					1544:1549	the inactivated yeasts	1528:1549	the inactivated yeasts (ICJ and IWA diets)	1528:1569	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	9	18	from	diversity	1647:1655	arg1	fish					1678:1681	fish	1678:1681	fish	1678:1681	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	5	19	dep	diets	856:860	arg1	diet					905:908	a challenging diet	891:908	a challenging diet	891:908	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	19	dep	diets	856:860	arg1	diet					885:888	a fishmeal (FM)-based diet	863:888	a fishmeal (FM)-based diet	863:888	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	20	theme	30	915:916	arg1	%					917:917	%	917:917	%	917:917	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	7	21	dep	RESULTS	1232:1238	arg1	composition					1254:1264	The microbial composition	1240:1264	The microbial composition	1240:1264	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	7	21	dep	RESULTS	1232:1238	arg1	capacity					1290:1297	predicted metabolic capacity	1270:1297	predicted metabolic capacity	1270:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	7	21	dep	RESULTS	1232:1238	arg1	RESULTS					1232:1238	RESULTS The microbial composition and predicted metabolic capacity	1232:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota	1232:1315	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	4	22	theme	direct	670:675	arg1	heat-inactivation					677:693	direct heat-inactivation	670:693	direct heat-inactivation with spray-drying	670:711	Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA).
37016467	9	23	from	richness	1634:1641	arg1	fish					1678:1681	fish	1678:1681	fish	1678:1681	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	6	24	theme	gut	1164:1166	arg1	microbiota					1168:1177	gut microbiota	1164:1177	gut microbiota	1164:1177	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	0	25	theme	yeast	10:14	arg1	species					16:22	yeast species	10:22	yeast species	10:22	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	12	26	dep	CONCLUSIONS	1944:1954	arg1	showed					1974:1979	showed	1974:1979	showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish	1974:2115	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	14	27	theme	degradation	2291:2301	arg1	pathway					2303:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	12	28	theme	dietary	1986:1992	arg1	inclusion					1994:2002	dietary inclusion	1986:2002	dietary inclusion of FM and SBM	1986:2016	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	0	29	theme	Atlantic	67:74	arg1	salmon					76:81	Atlantic salmon	67:81	Atlantic salmon (Salmo salar)	67:95	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	0	29	theme	Atlantic	67:74	arg1	salar					90:94	Salmo salar	84:94	Salmo salar	84:94	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	1	30	theme	BACKGROUND	139:148	arg1	Yeasts					150:155	BACKGROUND Yeasts	139:155	BACKGROUND Yeasts	139:155	BACKGROUND Yeasts are gaining attention as alternative ingredients in aquafeeds.
37016467	7	31	theme	SBM	1365:1367	arg1	diet					1369:1372	those fed SBM diet	1355:1372	those fed SBM diet	1355:1372	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	0	32	from	Effect	0:5	arg1	microbiota					53:62	intestinal microbiota	42:62	intestinal microbiota of Atlantic salmon (Salmo salar)	42:95	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	6	33	theme	predicted	1130:1138	arg1	capacities					1150:1159	the predicted metabolic capacities	1126:1159	the predicted metabolic capacities of gut microbiota	1126:1177	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	4	34	dep	produced	635:642	arg1	IWA					722:724	IWA	722:724	IWA	722:724	Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA).
37016467	4	34	dep	produced	635:642	arg1	ICJ					714:716	ICJ	714:716	ICJ	714:716	Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA).
37016467	5	35	contain	containing	944:953	arg2	%					969:969	10%	967:969	10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	967:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	35	contain	containing	944:953	arg1	diets					938:942	four other diets	927:942	four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	927:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	35	contain	containing	944:953	arg2	products					997:1004	the four yeast products	982:1004	the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	982:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	35	contain	containing	944:953	arg2	SBM					959:961	30% SBM	955:961	30% SBM	955:961	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	35	contain	containing	944:953	arg2	each					974:977	each	974:977	each	974:977	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	12	36	theme	present	1960:1966	arg1	study					1968:1972	The present study	1956:1972	The present study	1956:1972	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	10	37	from	increase	1786:1793	arg1	degradation					1813:1823	mucin O-glycan degradation	1798:1823	mucin O-glycan degradation	1798:1823	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	5	38	theme	30	955:956	arg1	%					957:957	%	957:957	%	957:957	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	0	39	theme	processing	28:37	arg1	Effect					0:5	Effect	0:5	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar)	0:95	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	5	40	theme	fishmeal	865:872	arg1	diet					905:908	a challenging diet	891:908	a challenging diet	891:908	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	40	theme	fishmeal	865:872	arg1	diet					885:888	a fishmeal (FM)-based diet	863:888	a fishmeal (FM)-based diet	863:888	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	11	41	theme	fish	1878:1881	arg1	microbiota					1864:1873	The gut microbiota	1856:1873	The gut microbiota of fish fed AWA diet	1856:1894	The gut microbiota of fish fed AWA diet was highly dominated by the family Bacillaceae.
37016467	2	42	from	impact	233:238	arg1	modulation					262:271	modulation	262:271	modulation of intestinal microbiota of fish fed plant-based ingredients	262:332	However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited.
37016467	6	43	theme	metabolic	1214:1222	arg1	models					1224:1229	genome-scale metabolic models	1201:1229	genome-scale metabolic models	1201:1229	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	7	44	theme	FM	1343:1344	arg1	diet					1346:1349	FM diet	1343:1349	FM diet	1343:1349	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	13	45	theme	inactivated	2135:2145	arg1	yeasts					2147:2152	inactivated yeasts	2135:2152	inactivated yeasts	2135:2152	The inclusion of inactivated yeasts did not alter the modulation caused by SBM-based diet.
37016467	8	46	from	composition	1489:1499	arg1	digesta					1508:1514	the digesta	1504:1514	the digesta of fish fed the inactivated yeasts (ICJ and IWA diets)	1504:1569	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	9	47	theme	autolyzed	1585:1593	arg1	AWA					1611:1613	AWA	1611:1613	AWA	1611:1613	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	9	47	theme	autolyzed	1585:1593	arg1	ACJ					1603:1605	ACJ	1603:1605	ACJ	1603:1605	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	9	47	theme	autolyzed	1585:1593	arg1	yeasts					1595:1600	autolyzed yeasts	1585:1600	autolyzed yeasts (ACJ and AWA diets)	1585:1620	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	5	48	theme	challenging	893:903	arg1	diet					905:908	a challenging diet	891:908	a challenging diet	891:908	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	48	theme	challenging	893:903	arg1	diet					885:888	a fishmeal (FM)-based diet	863:888	a fishmeal (FM)-based diet	863:888	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	14	49	theme	O-glycan	2282:2289	arg1	pathway					2303:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	2	50	theme	plant-based	310:320	arg1	ingredients					322:332	plant-based ingredients	310:332	plant-based ingredients	310:332	However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited.
37016467	7	51	theme	gut	1302:1304	arg1	microbiota					1306:1315	gut microbiota	1302:1315	gut microbiota	1302:1315	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	12	52	theme	microbiota	2098:2107	arg1	composition					2046:2056	composition	2046:2056	composition	2046:2056	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	12	52	theme	microbiota	2098:2107	arg1	capacity					2082:2089	predicted metabolic capacity	2062:2089	predicted metabolic capacity	2062:2089	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	3	53	theme	Atlantic	499:506	arg1	salmon					508:513	Atlantic salmon	499:513	Atlantic salmon smolt	499:519	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	8	54	theme	lactic	1436:1441	arg1	bacteria					1448:1455	lactic acid bacteria	1436:1455	lactic acid bacteria	1436:1455	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	11	55	theme	AWA	1887:1889	arg1	diet					1891:1894	AWA diet	1887:1894	AWA diet	1887:1894	The gut microbiota of fish fed AWA diet was highly dominated by the family Bacillaceae.
37016467	14	56	theme	Fish	2209:2212	arg1	diet					2222:2225	Fish fed ACJ diet	2209:2225	Fish fed ACJ diet	2209:2225	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	5	57	theme	-based	878:883	arg1	diet					905:908	a challenging diet	891:908	a challenging diet	891:908	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	57	theme	-based	878:883	arg1	diet					885:888	a fishmeal (FM)-based diet	863:888	a fishmeal (FM)-based diet	863:888	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	12	58	theme	fish	2112:2115	arg1	microbiota					2098:2107	gut microbiota	2094:2107	gut microbiota of fish	2094:2115	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	6	59	theme	samples	1068:1074	arg1	profiling					1047:1055	Microbial profiling	1037:1055	Microbial profiling of digesta samples	1037:1074	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	3	60	theme	microbiota	485:494	arg1	diversity					435:443	diversity	435:443	diversity	435:443	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	3	60	theme	microbiota	485:494	arg1	composition					422:432	composition	422:432	composition	422:432	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	3	60	theme	microbiota	485:494	arg1	capacity					469:476	predicted metabolic capacity	449:476	predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet	449:553	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	8	61	theme	bacteria	1448:1455	arg1	members					1425:1431	members	1425:1431	members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets)	1425:1569	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	7	62	theme	predicted	1270:1278	arg1	capacity					1290:1297	predicted metabolic capacity	1270:1297	predicted metabolic capacity	1270:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	7	62	theme	predicted	1270:1278	arg1	RESULTS					1232:1238	RESULTS The microbial composition and predicted metabolic capacity	1232:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota	1232:1315	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	10	63	theme	mucin	1798:1802	arg1	degradation					1813:1823	mucin O-glycan degradation	1798:1823	mucin O-glycan degradation	1798:1823	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	12	64	theme	metabolic	2072:2080	arg1	capacity					2082:2089	predicted metabolic capacity	2062:2089	predicted metabolic capacity	2062:2089	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	12	65	theme	SBM	2014:2016	arg1	inclusion					1994:2002	dietary inclusion	1986:2002	dietary inclusion of FM and SBM	1986:2016	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	10	66	theme	other	1843:1847	arg1	diets					1849:1853	the other diets	1839:1853	the other diets	1839:1853	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	6	67	theme	Microbial	1037:1045	arg1	profiling					1047:1055	Microbial profiling	1037:1055	Microbial profiling of digesta samples	1037:1074	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	3	68	theme	smolt	515:519	arg1	microbiota					485:494	gut microbiota	481:494	gut microbiota of Atlantic salmon smolt	481:519	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	9	69	theme	yeasts	1595:1600	arg1	Inclusion					1572:1580	Inclusion	1572:1580	Inclusion of autolyzed yeasts (ACJ and AWA diets)	1572:1620	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	11	70	theme	gut	1860:1862	arg1	microbiota					1864:1873	The gut microbiota	1856:1873	The gut microbiota of fish fed AWA diet	1856:1894	The gut microbiota of fish fed AWA diet was highly dominated by the family Bacillaceae.
37016467	2	71	theme	fish	301:304	arg1	microbiota					287:296	intestinal microbiota	276:296	intestinal microbiota of fish fed plant-based ingredients	276:332	However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited.
37016467	8	72	theme	microbial	1479:1487	arg1	composition					1489:1499	microbial composition	1479:1499	microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets)	1479:1569	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	4	73	dep	autolyzed	730:738	arg1	AWA					793:795	AWA	793:795	AWA	793:795	Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA).
37016467	4	73	dep	autolyzed	730:738	arg1	ACJ					785:787	ACJ	785:787	ACJ	785:787	Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA).
37016467	4	73	dep	autolyzed	730:738	arg1	followed					759:766	followed	759:766	followed by spray-drying	759:782	Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA).
37016467	9	74	theme	gut	1660:1662	arg1	microbiota					1664:1673	gut microbiota	1660:1673	gut microbiota	1660:1673	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	10	75	theme	gut	1688:1690	arg1	microbiota					1692:1701	The gut microbiota	1684:1701	The gut microbiota of fish	1684:1709	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	0	76	theme	soybean	101:107	arg1	diets					120:124	soybean meal-based diets	101:124	soybean meal-based diets	101:124	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	2	77	theme	microbiota	287:296	arg1	modulation					262:271	modulation	262:271	modulation of intestinal microbiota of fish fed plant-based ingredients	262:332	However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited.
37016467	5	78	theme	feeding	811:817	arg1	experiment					819:828	a 42-day feeding experiment	802:828	a 42-day feeding experiment	802:828	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	6	79	theme	gene	1105:1108	arg1	sequencing					1110:1119	16S rRNA gene sequencing	1096:1119	16S rRNA gene sequencing	1096:1119	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	14	80	theme	mucin	2276:2280	arg1	pathway					2303:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Pediococcus, and mucin O-glycan degradation pathway	2259:2309	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	12	81	theme	predicted	2062:2070	arg1	capacity					2082:2089	predicted metabolic capacity	2062:2089	predicted metabolic capacity	2062:2089	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	6	82	theme	metabolic	1140:1148	arg1	capacities					1150:1159	the predicted metabolic capacities	1126:1159	the predicted metabolic capacities of gut microbiota	1126:1177	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	6	83	theme	16S	1096:1098	arg1	sequencing					1110:1119	16S rRNA gene sequencing	1096:1119	16S rRNA gene sequencing	1096:1119	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	5	84	theme	other	932:936	arg1	diets					938:942	four other diets	927:942	four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	927:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	8	85	theme	fish	1519:1522	arg1	digesta					1508:1514	the digesta	1504:1514	the digesta of fish fed the inactivated yeasts (ICJ and IWA diets)	1504:1569	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	10	86	dep	fed	1711:1713	arg1	dominated					1728:1736	dominated	1728:1736	was dominated by the genus Pediococcus	1724:1761	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	10	86	dep	fed	1711:1713	arg1	showed					1767:1772	showed	1767:1772	showed a predicted increase in mucin O-glycan degradation compared with the other diets	1767:1853	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	14	87	theme	other	2329:2333	arg1	diets					2335:2339	the other diets	2325:2339	the other diets	2325:2339	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	13	88	theme	SBM-based	2193:2201	arg1	diet					2203:2206	SBM-based diet	2193:2206	SBM-based diet	2193:2206	The inclusion of inactivated yeasts did not alter the modulation caused by SBM-based diet.
37016467	5	89	dep	ICJ	1013:1015	arg1	i.e.					1007:1010	i.e.	1007:1010	i.e.	1007:1010	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	90	dep	fishmeal	865:872	arg1	FM					875:876	FM	875:876	FM	875:876	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	4	91	with	heat-inactivation	677:693	arg1	spray-drying					700:711	spray-drying	700:711	spray-drying	700:711	Two yeasts, Cyberlindnera jadinii (CJ) and Wickerhamomyces anomalus (WA), were produced in-house and processed by direct heat-inactivation with spray-drying (ICJ and IWA) or autolyzed at 50 °C for 16 h, followed by spray-drying (ACJ and AWA).
37016467	2	92	theme	yeast	243:247	arg1	inclusion					249:257	yeast inclusion	243:257	yeast inclusion	243:257	However, the impact of yeast inclusion on modulation of intestinal microbiota of fish fed plant-based ingredients is limited.
37016467	3	93	theme	metabolic	459:467	arg1	capacity					469:476	predicted metabolic capacity	449:476	predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet	449:553	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	6	94	theme	microbiota	1168:1177	arg1	capacities					1150:1159	the predicted metabolic capacities	1126:1159	the predicted metabolic capacities of gut microbiota	1126:1177	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	5	95	theme	%	917:917	arg1	SBM					919:921	30% SBM	915:921	30% SBM	915:921	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	14	96	theme	pathway	2303:2309	arg1	abundance					2246:2254	relative abundance	2237:2254	relative abundance of Pediococcus, and mucin O-glycan degradation pathway	2237:2309	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	11	97	theme	family	1924:1929	arg1	Bacillaceae					1931:1941	the family Bacillaceae	1920:1941	the family Bacillaceae	1920:1941	The gut microbiota of fish fed AWA diet was highly dominated by the family Bacillaceae.
37016467	5	98	dep	products	997:1004	arg1	ICJ					1013:1015	ICJ	1013:1015	ICJ	1013:1015	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	98	dep	products	997:1004	arg1	products					997:1004	the four yeast products	982:1004	the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	982:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	98	dep	products	997:1004	arg1	AWA					1031:1033	AWA	1031:1033	AWA	1031:1033	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	98	dep	products	997:1004	arg1	IWA					1023:1025	IWA	1023:1025	IWA	1023:1025	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	0	99	theme	salmon	76:81	arg1	microbiota					53:62	intestinal microbiota	42:62	intestinal microbiota of Atlantic salmon (Salmo salar)	42:95	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	3	100	from	effects	387:393	arg1	diversity					435:443	diversity	435:443	diversity	435:443	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	3	100	from	effects	387:393	arg1	composition					422:432	composition	422:432	composition	422:432	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	3	100	from	effects	387:393	arg1	capacity					469:476	predicted metabolic capacity	449:476	predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet	449:553	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	5	101	theme	42-day	804:809	arg1	experiment					819:828	a 42-day feeding experiment	802:828	a 42-day feeding experiment	802:828	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	7	102	theme	fed	1361:1363	arg1	diet					1369:1372	those fed SBM diet	1355:1372	those fed SBM diet	1355:1372	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	12	103	theme	FM	2007:2008	arg1	inclusion					1994:2002	dietary inclusion	1986:2002	dietary inclusion of FM and SBM	1986:2016	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	14	104	theme	ACJ	2218:2220	arg1	diet					2222:2225	Fish fed ACJ diet	2209:2225	Fish fed ACJ diet	2209:2225	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	0	105	theme	Salmo	84:88	arg1	salmon					76:81	Atlantic salmon	67:81	Atlantic salmon (Salmo salar)	67:95	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	0	105	theme	Salmo	84:88	arg1	salar					90:94	Salmo salar	84:94	Salmo salar	84:94	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	8	106	theme	IWA	1560:1562	arg1	diets					1564:1568	IWA diets	1560:1568	IWA diets	1560:1568	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	13	107	theme	yeasts	2147:2152	arg1	inclusion					2122:2130	The inclusion	2118:2130	The inclusion of inactivated yeasts	2118:2152	The inclusion of inactivated yeasts did not alter the modulation caused by SBM-based diet.
37016467	9	108	dep	ACJ	1603:1605	arg1	diets					1615:1619	diets	1615:1619	diets	1615:1619	Inclusion of autolyzed yeasts (ACJ and AWA diets) reduced the richness and diversity of gut microbiota in fish.
37016467	12	109	dep	composition	2046:2056	arg1	the					2042:2044	the	2042:2044	the	2042:2044	CONCLUSIONS The present study showed that dietary inclusion of FM and SBM differentially modulate the composition and predicted metabolic capacity of gut microbiota of fish.
37016467	5	110	theme	%	957:957	arg1	SBM					959:961	30% SBM	955:961	30% SBM	955:961	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	14	111	theme	fed	2214:2216	arg1	diet					2222:2225	Fish fed ACJ diet	2209:2225	Fish fed ACJ diet	2209:2225	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	3	112	theme	processing	408:417	arg1	effects					387:393	the effects	383:393	the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet	383:553	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	0	113	theme	species	16:22	arg1	Effect					0:5	Effect	0:5	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar)	0:95	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	5	114	theme	yeast	991:995	arg1	ICJ					1013:1015	ICJ	1013:1015	ICJ	1013:1015	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	114	theme	yeast	991:995	arg1	IWA					1023:1025	IWA	1023:1025	IWA	1023:1025	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	114	theme	yeast	991:995	arg1	AWA					1031:1033	AWA	1031:1033	AWA	1031:1033	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	114	theme	yeast	991:995	arg1	products					997:1004	the four yeast products	982:1004	the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	982:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	3	115	theme	present	356:362	arg1	study					364:368	the present study	352:368	the present study	352:368	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	3	116	theme	predicted	449:457	arg1	capacity					469:476	predicted metabolic capacity	449:476	predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet	449:553	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	6	117	theme	genome-scale	1201:1212	arg1	models					1224:1229	genome-scale metabolic models	1201:1229	genome-scale metabolic models	1201:1229	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	3	118	theme	yeast	398:402	arg1	effects					387:393	the effects	383:393	the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet	383:553	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	0	119	theme	intestinal	42:51	arg1	microbiota					53:62	intestinal microbiota	42:62	intestinal microbiota of Atlantic salmon (Salmo salar)	42:95	Effect of yeast species and processing on intestinal microbiota of Atlantic salmon (Salmo salar) fed soybean meal-based diets in seawater.
37016467	5	120	theme	each	974:977	arg1	products					997:1004	the four yeast products	982:1004	the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	982:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	120	theme	each	974:977	arg1	each					974:977	each	974:977	each	974:977	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	120	theme	each	974:977	arg1	SBM					959:961	30% SBM	955:961	30% SBM	955:961	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	5	120	theme	each	974:977	arg1	%					969:969	10%	967:969	10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA)	967:1034	In a 42-day feeding experiment, fish were fed one of six diets: a fishmeal (FM)-based diet, a challenging diet with 30% SBM and four other diets containing 30% SBM and 10% of each of the four yeast products (i.e., ICJ, ACJ, IWA and AWA).
37016467	14	121	theme	relative	2237:2244	arg1	abundance					2246:2254	relative abundance	2237:2254	relative abundance of Pediococcus, and mucin O-glycan degradation pathway	2237:2309	Fish fed ACJ diet increased relative abundance of Pediococcus, and mucin O-glycan degradation pathway compared with the other diets.
37016467	6	122	theme	rRNA	1100:1103	arg1	sequencing					1110:1119	16S rRNA gene sequencing	1096:1119	16S rRNA gene sequencing	1096:1119	Microbial profiling of digesta samples was conducted using 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models.
37016467	3	123	theme	gut	481:483	arg1	microbiota					485:494	gut microbiota	481:494	gut microbiota of Atlantic salmon smolt	481:519	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
37016467	10	124	theme	predicted	1776:1784	arg1	increase					1786:1793	a predicted increase	1774:1793	a predicted increase in mucin O-glycan degradation	1774:1823	The gut microbiota of fish fed ACJ diet was dominated by the genus Pediococcus and showed a predicted increase in mucin O-glycan degradation compared with the other diets.
37016467	8	125	theme	fish	1390:1393	arg1	digesta					1379:1385	The digesta	1375:1385	The digesta of fish fed SBM diet	1375:1406	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	8	126	theme	acid	1443:1446	arg1	bacteria					1448:1455	lactic acid bacteria	1436:1455	lactic acid bacteria	1436:1455	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	7	127	theme	microbiota	1306:1315	arg1	composition					1254:1264	The microbial composition	1240:1264	The microbial composition	1240:1264	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	7	127	theme	microbiota	1306:1315	arg1	capacity					1290:1297	predicted metabolic capacity	1270:1297	predicted metabolic capacity	1270:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	7	127	theme	microbiota	1306:1315	arg1	RESULTS					1232:1238	RESULTS The microbial composition and predicted metabolic capacity	1232:1297	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota	1232:1315	RESULTS The microbial composition and predicted metabolic capacity of gut microbiota differed between fish fed FM diet and those fed SBM diet.
37016467	8	128	dep	yeasts	1544:1549	arg1	ICJ					1552:1554	ICJ	1552:1554	ICJ	1552:1554	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	8	128	dep	yeasts	1544:1549	arg1	diets					1564:1568	IWA diets	1560:1568	IWA diets	1560:1568	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	8	129	theme	SBM	1399:1401	arg1	diet					1403:1406	SBM diet	1399:1406	SBM diet	1399:1406	The digesta of fish fed SBM diet was dominated by members of lactic acid bacteria, which was similar to microbial composition in the digesta of fish fed the inactivated yeasts (ICJ and IWA diets).
37016467	3	130	theme	salmon	508:513	arg1	smolt					515:519	Atlantic salmon smolt	499:519	Atlantic salmon smolt	499:519	Thus, the present study investigates the effects of yeast and processing on composition, diversity and predicted metabolic capacity of gut microbiota of Atlantic salmon smolt fed soybean meal (SBM)-based diet.
34990680	6	0	theme	primary	1195:1201	arg1	settler					1203:1209	the primary settler	1191:1209	the primary settler	1191:1209	Solids captured with the primary settler or micro-sieve consisted primarily of polysaccharides, and led to the establishment of a microbial community specialized in the degradation of complex carbohydrates.
34990680	7	1	with	simple	1421:1426	arg1	propionate					1446:1455	propionate	1446:1455	propionate	1446:1455	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	7	1	with	simple	1421:1426	arg1	acetate					1434:1440	acetate	1434:1440	acetate	1434:1440	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	5	2	theme	community	1120:1128	arg1	complexity					1080:1089	the complexity	1076:1089	the complexity of the fermenter's microbial community and ultimately, of the VFA composition	1076:1167	Our study demonstrates that the capture technology strongly influences the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition.
34990680	8	3	theme	higher	1564:1569	arg1	contents					1579:1586	higher protein contents	1564:1586	higher protein contents	1564:1586	In contrast, the HRAS system produced biomass-rich solids associated with higher protein contents.
34990680	9	4	theme	microbial	1593:1601	arg1	community					1603:1611	The microbial community	1589:1611	The microbial community which then developed in the fermenter	1589:1649	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	9	4	theme	microbial	1593:1601	arg1	diversified					1670:1680	diversified	1670:1680	diversified	1670:1680	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	10	5	theme	equal	1842:1846	arg1	fractions					1848:1856	equal fractions	1842:1856	equal fractions of isoacids and propionate (both ~20%)	1842:1895	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	5	6	theme	microbial	1110:1118	arg1	community					1120:1128	the fermenter's microbial community	1094:1128	the fermenter's microbial community	1094:1128	Our study demonstrates that the capture technology strongly influences the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition.
34990680	7	7	theme	produced	1381:1388	arg1	composition					1394:1404	The produced VFA composition	1377:1404	The produced VFA composition	1377:1404	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	7	7	theme	produced	1381:1388	arg1	simple					1421:1426	simple	1421:1426	simple	1421:1426	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	11	8	theme	VFA	1993:1995	arg1	composition					1997:2007	the VFA composition	1989:2007	the VFA composition	1989:2007	Finally, TAP did not significantly modify the VFA composition while increasing VFA yields on HRAS and sieved material by 35% and 20%, respectively.
34990680	12	9	from	MWW	2197:2199	arg1	substrates					2181:2190	organic substrates	2173:2190	organic substrates from MWW	2173:2199	Overall, we demonstrated that the selection of the technology used to capture organic substrates from MWW governs the composition of the VFA cocktail, ultimately with implications for their further utilization.
34990680	3	10	theme	VFA	565:567	arg1	composition					569:579	VFA composition	565:579	VFA composition produced by fermentation	565:604	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	3	10	theme	VFA	565:567	arg1	as					673:674	as	673:674	as	673:674	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	4	11	theme	sludge	848:853	arg1	system					862:867	a high-rate activated sludge (HRAS) system	826:867	a high-rate activated sludge (HRAS) system	826:867	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	3	12	dep	influenced	609:618	arg1	i					621:621	i	621:621	i	621:621	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	4	13	theme	HRAS	856:859	arg1	system					862:867	a high-rate activated sludge (HRAS) system	826:867	a high-rate activated sludge (HRAS) system	826:867	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	0	14	theme	fatty	139:143	arg1	profile					150:156	volatile fatty acid profile	130:156	volatile fatty acid profile	130:156	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	11	15	theme	VFA	2026:2028	arg1	yields					2030:2035	VFA yields	2026:2035	VFA yields	2026:2035	Finally, TAP did not significantly modify the VFA composition while increasing VFA yields on HRAS and sieved material by 35% and 20%, respectively.
34990680	3	16	theme	alkaline	703:710	arg1	pre-treatment					712:724	thermal alkaline pre-treatment	695:724	thermal alkaline pre-treatment (TAP)	695:730	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	3	16	theme	alkaline	703:710	arg1	TAP					727:729	TAP	727:729	TAP	727:729	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	4	17	theme	high-rate	828:836	arg1	system					862:867	a high-rate activated sludge (HRAS) system	826:867	a high-rate activated sludge (HRAS) system	826:867	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	0	18	theme	anaerobic	165:173	arg1	fermentation					175:186	anaerobic fermentation	165:186	anaerobic fermentation	165:186	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	4	19	theme	continuous	889:898	arg1	fermenters					910:919	continuous lab-scale fermenters	889:919	continuous lab-scale fermenters	889:919	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	6	20	theme	microbial	1300:1308	arg1	community					1310:1318	a microbial community	1298:1318	a microbial community specialized in the degradation of complex carbohydrates	1298:1374	Solids captured with the primary settler or micro-sieve consisted primarily of polysaccharides, and led to the establishment of a microbial community specialized in the degradation of complex carbohydrates.
34990680	5	21	theme	solids	1047:1052	arg1	composition					1019:1029	the composition	1015:1029	the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition	1015:1167	Our study demonstrates that the capture technology strongly influences the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition.
34990680	10	22	dep	%	1894:1894	arg1	both					1886:1889	both	1886:1889	both	1886:1889	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	10	23	theme	dominant	1925:1932	arg1	acid					1934:1937	the dominant acid	1921:1937	the dominant acid (~50%)	1921:1944	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	10	23	theme	dominant	1925:1932	arg1	%					1943:1943	~50%	1940:1943	~50%	1940:1943	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	2	24	theme	VFA	491:493	arg1	production					495:504	VFA production	491:504	VFA production	491:504	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	2	25	used	used	359:362	arg2	technologies					339:350	different capture technologies	321:350	different capture technologies	321:350	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	0	26	theme	community	106:114	arg1	structure					116:124	microbial community structure	96:124	microbial community structure	96:124	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	9	27	theme	substrates	1725:1734	arg1	range					1716:1720	a wider range	1708:1720	a wider range of substrates (polysaccharides, proteins, amino acids)	1708:1775	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	0	28	theme	solids	68:73	arg1	Composition					32:42	Composition	32:42	Composition of municipal wastewater solids	32:73	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	6	29	theme	carbohydrates	1362:1374	arg1	degradation					1339:1349	the degradation	1335:1349	the degradation of complex carbohydrates	1335:1374	Solids captured with the primary settler or micro-sieve consisted primarily of polysaccharides, and led to the establishment of a microbial community specialized in the degradation of complex carbohydrates.
34990680	1	30	theme	municipal	276:284	arg1	MWW					298:300	MWW	298:300	MWW	298:300	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	1	30	theme	municipal	276:284	arg1	wastewater					286:295	municipal wastewater	276:295	municipal wastewater (MWW)	276:301	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	10	31	with	complex	1828:1834	arg1	fractions					1848:1856	equal fractions	1842:1856	equal fractions of isoacids and propionate (both ~20%)	1842:1895	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	2	32	theme	solids	417:422	arg1	form					409:412	form	409:412	form of solids	409:422	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	0	33	theme	technology	12:21	arg1	matters					23:29	The capture technology matters	0:29	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.	0:187	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	2	34	theme	solids	454:459	arg1	pre-treatment					431:443	pre-treatment	431:443	pre-treatment of those solids	431:459	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	12	35	theme	further	2285:2291	arg1	utilization					2293:2303	their further utilization	2279:2303	their further utilization	2279:2303	Overall, we demonstrated that the selection of the technology used to capture organic substrates from MWW governs the composition of the VFA cocktail, ultimately with implications for their further utilization.
34990680	9	36	theme	amino	1764:1768	arg1	polysaccharides					1737:1751	polysaccharides	1737:1751	polysaccharides	1737:1751	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	9	36	theme	amino	1764:1768	arg1	acids					1770:1774	amino acids	1764:1774	amino acids	1764:1774	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	0	37	theme	wastewater	57:66	arg1	solids					68:73	municipal wastewater solids	47:73	municipal wastewater solids	47:73	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	4	38	theme	solids	764:769	arg1	fermentation					748:759	the fermentation	744:759	the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system	744:867	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	2	39	theme	capture	331:337	arg1	technologies					339:350	different capture technologies	321:350	different capture technologies	321:350	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	10	40	theme	VFA	1803:1805	arg1	complex					1828:1834	complex	1828:1834	complex	1828:1834	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	10	40	theme	VFA	1803:1805	arg1	composition					1807:1817	the produced VFA composition	1790:1817	the produced VFA composition	1790:1817	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	8	41	theme	protein	1571:1577	arg1	contents					1579:1586	higher protein contents	1564:1586	higher protein contents	1564:1586	In contrast, the HRAS system produced biomass-rich solids associated with higher protein contents.
34990680	2	42	from	wastewater	395:404	arg1	form					409:412	form	409:412	form of solids	409:422	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	8	43	theme	HRAS	1507:1510	arg1	system					1512:1517	the HRAS system	1503:1517	the HRAS system	1503:1517	In contrast, the HRAS system produced biomass-rich solids associated with higher protein contents.
34990680	12	44	theme	VFA	2232:2234	arg1	cocktail					2236:2243	the VFA cocktail	2228:2243	the VFA cocktail	2228:2243	Overall, we demonstrated that the selection of the technology used to capture organic substrates from MWW governs the composition of the VFA cocktail, ultimately with implications for their further utilization.
34990680	1	45	theme	relevant	248:255	arg1	option					257:262	a relevant option	246:262	a relevant option to valorize municipal wastewater (MWW)	246:301	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	4	46	theme	primary	790:796	arg1	settler					798:804	a primary settler	788:804	a primary settler	788:804	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	9	47	dep	substrates	1725:1734	arg1	polysaccharides					1737:1751	polysaccharides	1737:1751	polysaccharides	1737:1751	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	9	47	dep	substrates	1725:1734	arg1	proteins					1754:1761	proteins	1754:1761	proteins	1754:1761	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	9	47	dep	substrates	1725:1734	arg1	acids					1770:1774	amino acids	1764:1774	amino acids	1764:1774	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	7	48	theme	VFA	1390:1392	arg1	composition					1394:1404	The produced VFA composition	1377:1404	The produced VFA composition	1377:1404	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	7	48	theme	VFA	1390:1392	arg1	simple					1421:1426	simple	1421:1426	simple	1421:1426	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	3	49	theme	thermal	695:701	arg1	pre-treatment					712:724	thermal alkaline pre-treatment	695:724	thermal alkaline pre-treatment (TAP)	695:730	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	3	49	theme	thermal	695:701	arg1	TAP					727:729	TAP	727:729	TAP	727:729	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	0	50	theme	volatile	130:137	arg1	profile					150:156	volatile fatty acid profile	130:156	volatile fatty acid profile	130:156	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	1	51	theme	volatile	207:214	arg1	VFAs					229:232	VFAs	229:232	VFAs	229:232	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	1	51	theme	volatile	207:214	arg1	acids					222:226	volatile fatty acids	207:226	volatile fatty acids (VFAs)	207:233	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	4	52	theme	activated	838:846	arg1	system					862:867	a high-rate activated sludge (HRAS) system	826:867	a high-rate activated sludge (HRAS) system	826:867	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	12	53	theme	organic	2173:2179	arg1	substrates					2181:2190	organic substrates	2173:2190	organic substrates from MWW	2173:2199	Overall, we demonstrated that the selection of the technology used to capture organic substrates from MWW governs the composition of the VFA cocktail, ultimately with implications for their further utilization.
34990680	10	54	theme	isoacids	1861:1868	arg1	fractions					1848:1856	equal fractions	1842:1856	equal fractions of isoacids and propionate (both ~20%)	1842:1895	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	0	55	theme	acid	145:148	arg1	profile					150:156	volatile fatty acid profile	130:156	volatile fatty acid profile	130:156	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	1	56	theme	acids	222:226	arg1	production					193:202	The production	189:202	The production of volatile fatty acids (VFAs)	189:233	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	2	57	theme	subsequent	513:522	arg1	fermentation					524:535	subsequent fermentation	513:535	subsequent fermentation	513:535	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	10	58	theme	propionate	1874:1883	arg1	fractions					1848:1856	equal fractions	1842:1856	equal fractions of isoacids and propionate (both ~20%)	1842:1895	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	12	59	theme	technology	2146:2155	arg1	selection					2129:2137	the selection	2125:2137	the selection of the technology used to capture organic substrates from MWW	2125:2199	Overall, we demonstrated that the selection of the technology used to capture organic substrates from MWW governs the composition of the VFA cocktail, ultimately with implications for their further utilization.
34990680	3	60	theme	technology	653:662	arg1	choice					631:636	the choice	627:636	the choice of the capture technology	627:662	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	3	61	theme	pre-treatment	712:724	arg1	use					688:690	the use	684:690	the use of thermal alkaline pre-treatment (TAP)	684:730	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	6	62	theme	community	1310:1318	arg1	establishment					1281:1293	the establishment	1277:1293	the establishment of a microbial community specialized in the degradation of complex carbohydrates	1277:1374	Solids captured with the primary settler or micro-sieve consisted primarily of polysaccharides, and led to the establishment of a microbial community specialized in the degradation of complex carbohydrates.
34990680	5	63	theme	capture	976:982	arg1	technology					984:993	the capture technology	972:993	the capture technology	972:993	Our study demonstrates that the capture technology strongly influences the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition.
34990680	5	64	theme	composition	1157:1167	arg1	complexity					1080:1089	the complexity	1076:1089	the complexity of the fermenter's microbial community and ultimately, of the VFA composition	1076:1167	Our study demonstrates that the capture technology strongly influences the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition.
34990680	7	65	theme	VFAs	1484:1487	arg1	VFAs					1484:1487	the VFAs	1480:1487	the VFAs	1480:1487	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	7	65	theme	VFAs	1484:1487	arg1	%					1475:1475	>90%	1472:1475	>90% of the VFAs	1472:1487	The produced VFA composition was relatively simple, with acetate and propionate accounting for >90% of the VFAs.
34990680	4	66	theme	lab-scale	900:908	arg1	fermenters					910:919	continuous lab-scale fermenters	889:919	continuous lab-scale fermenters	889:919	Therefore, the fermentation of solids originating from a primary settler, a micro-sieve, and a high-rate activated sludge (HRAS) system was investigated in continuous lab-scale fermenters, with and without TAP.
34990680	0	67	theme	microbial	96:104	arg1	structure					116:124	microbial community structure	96:124	microbial community structure	96:124	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	9	68	theme	wider	1710:1714	arg1	range					1716:1720	a wider range	1708:1720	a wider range of substrates (polysaccharides, proteins, amino acids)	1708:1775	The microbial community which then developed in the fermenter was therefore more diversified and capable of converting a wider range of substrates (polysaccharides, proteins, amino acids).
34990680	0	69	theme	structure	116:124	arg1	complexity					82:91	complexity	82:91	complexity of microbial community structure	82:124	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	0	69	theme	structure	116:124	arg1	profile					150:156	volatile fatty acid profile	130:156	volatile fatty acid profile	130:156	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	1	70	theme	fatty	216:220	arg1	VFAs					229:232	VFAs	229:232	VFAs	229:232	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	1	70	theme	fatty	216:220	arg1	acids					222:226	volatile fatty acids	207:226	volatile fatty acids (VFAs)	207:233	The production of volatile fatty acids (VFAs) represents a relevant option to valorize municipal wastewater (MWW).
34990680	2	71	theme	organic	375:381	arg1	carbon					383:388	organic carbon	375:388	organic carbon	375:388	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	0	72	theme	capture	4:10	arg1	matters					23:29	The capture technology matters	0:29	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.	0:187	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	5	73	theme	produced	1038:1045	arg1	solids					1047:1052	the produced solids	1034:1052	the produced solids	1034:1052	Our study demonstrates that the capture technology strongly influences the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition.
34990680	11	74	theme	HRAS	2040:2043	arg1	material					2056:2063	HRAS and sieved material	2040:2063	HRAS and sieved material	2040:2063	Finally, TAP did not significantly modify the VFA composition while increasing VFA yields on HRAS and sieved material by 35% and 20%, respectively.
34990680	6	75	theme	complex	1354:1360	arg1	carbohydrates					1362:1374	complex carbohydrates	1354:1374	complex carbohydrates	1354:1374	Solids captured with the primary settler or micro-sieve consisted primarily of polysaccharides, and led to the establishment of a microbial community specialized in the degradation of complex carbohydrates.
34990680	8	76	theme	biomass-rich	1528:1539	arg1	solids					1541:1546	biomass-rich solids	1528:1546	biomass-rich solids associated with higher protein contents	1528:1586	In contrast, the HRAS system produced biomass-rich solids associated with higher protein contents.
34990680	2	77	contain	has	461:463	arg1	pre-treatment					431:443	pre-treatment	431:443	pre-treatment of those solids	431:459	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	2	77	contain	has	461:463	arg2	potential					469:477	the potential to increase VFA production during subsequent fermentation	465:535	the potential to increase VFA production during subsequent fermentation	465:535	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	0	78	theme	municipal	47:55	arg1	solids					68:73	municipal wastewater solids	47:73	municipal wastewater solids	47:73	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	11	79	theme	sieved	2049:2054	arg1	material					2056:2063	HRAS and sieved material	2040:2063	HRAS and sieved material	2040:2063	Finally, TAP did not significantly modify the VFA composition while increasing VFA yields on HRAS and sieved material by 35% and 20%, respectively.
34990680	3	80	theme	capture	645:651	arg1	technology					653:662	the capture technology	641:662	the capture technology	641:662	Our study investigates how VFA composition produced by fermentation is influenced (i) by the choice of the capture technology, as well as (ii) by the use of thermal alkaline pre-treatment (TAP).
34990680	2	81	theme	different	321:329	arg1	technologies					339:350	different capture technologies	321:350	different capture technologies	321:350	In this context, different capture technologies can be used to recover organic carbon from wastewater in form of solids, while pre-treatment of those solids has the potential to increase VFA production during subsequent fermentation.
34990680	0	82	dep	matters	23:29	arg1	drives					75:80	drives	75:80	drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation	75:186	The capture technology matters: Composition of municipal wastewater solids drives complexity of microbial community structure and volatile fatty acid profile during anaerobic fermentation.
34990680	10	83	theme	produced	1794:1801	arg1	complex					1828:1834	complex	1828:1834	complex	1828:1834	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	10	83	theme	produced	1794:1801	arg1	composition					1807:1817	the produced VFA composition	1790:1817	the produced VFA composition	1790:1817	Ultimately, the produced VFA composition was more complex, with equal fractions of isoacids and propionate (both ~20%), while acetate remained the dominant acid (~50%).
34990680	12	84	theme	cocktail	2236:2243	arg1	composition					2213:2223	the composition	2209:2223	the composition of the VFA cocktail	2209:2243	Overall, we demonstrated that the selection of the technology used to capture organic substrates from MWW governs the composition of the VFA cocktail, ultimately with implications for their further utilization.
34990680	5	85	theme	VFA	1153:1155	arg1	composition					1157:1167	the VFA composition	1149:1167	the VFA composition	1149:1167	Our study demonstrates that the capture technology strongly influences the composition of the produced solids, which in turn drives the complexity of the fermenter's microbial community and ultimately, of the VFA composition.
36301224	4	0	theme	present	1008:1014	arg1	polysaccharides					992:1006	the polysaccharides	988:1006	the polysaccharides present in the cell walls of surficial algal tissue	988:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	5	1	theme	different	1222:1230	arg1	polysaccharides					1232:1246	different polysaccharides	1222:1246	different polysaccharides across CCA taxa	1222:1262	Our results revealed dissimilarity among species' monosaccharide constituents, which suggests that organic matrices are composed of different polysaccharides across CCA taxa.
36301224	4	2	theme	surficial	850:858	arg1	composition					875:885	surficial monosaccharide composition	850:885	surficial monosaccharide composition	850:885	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	5	3	theme	monosaccharide	1140:1153	arg1	constituents					1155:1166	species' monosaccharide constituents	1131:1166	species' monosaccharide constituents	1131:1166	Our results revealed dissimilarity among species' monosaccharide constituents, which suggests that organic matrices are composed of different polysaccharides across CCA taxa.
36301224	3	4	theme	experimentation	804:818	arg1	2 months					792:799	2 months	792:799	2 months of experimentation	792:818	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	2	5	theme	CCA	483:485	arg1	taxa					487:490	CCA taxa	483:490	CCA taxa	483:490	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	3	6	theme	elevated	681:688	arg1	pCO2					690:693	elevated pCO2	681:693	elevated pCO2	681:693	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	4	7	attach	present	1008:1014	arg2	polysaccharides					992:1006	the polysaccharides	988:1006	the polysaccharides present in the cell walls of surficial algal tissue	988:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	4	7	attach	present	1008:1014	arg1	walls					1028:1032	the cell walls	1019:1032	the cell walls of surficial algal tissue	1019:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	5	8	theme	CCA	1255:1257	arg1	taxa					1259:1262	CCA taxa	1255:1262	CCA taxa	1255:1262	Our results revealed dissimilarity among species' monosaccharide constituents, which suggests that organic matrices are composed of different polysaccharides across CCA taxa.
36301224	1	9	theme	calcium	258:264	arg1	carbonate					266:274	calcium carbonate	258:274	calcium carbonate	258:274	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	0	10	theme	global	102:107	arg1	change					109:114	global change	102:114	global change	102:114	Cell wall organic matrix composition and biomineralization across reef-building coralline algae under global change.
36301224	4	11	from	variability	835:845	arg1	biomineralization					891:907	biomineralization	891:907	biomineralization	891:907	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	4	11	from	variability	835:845	arg1	composition					875:885	surficial monosaccharide composition	850:885	surficial monosaccharide composition	850:885	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	4	12	theme	monosaccharide	860:873	arg1	composition					875:885	surficial monosaccharide composition	850:885	surficial monosaccharide composition	850:885	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	7	13	theme	crucial	1497:1503	arg1	role					1505:1508	a crucial role	1495:1508	a crucial role	1495:1508	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	6	14	dep	ocean	1346:1350	arg1	response					1334:1341	response	1334:1341	response	1334:1341	We also observed that species differentially modulate composition in response to ocean acidification and warming.
36301224	6	14	dep	ocean	1346:1350	arg1	acidification					1352:1364	acidification	1352:1364	acidification	1352:1364	We also observed that species differentially modulate composition in response to ocean acidification and warming.
36301224	2	15	theme	acidification	636:648	arg1	threats					619:625	the threats	615:625	the threats of ocean acidification (OA) and warming	615:665	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	7	16	from	variability	1410:1420	arg1	composition					1425:1435	composition	1425:1435	composition	1425:1435	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	4	17	theme	monosaccharide	958:971	arg1	composition					973:983	the monosaccharide composition	954:983	the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue	954:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	7	18	theme	surficial	1513:1521	arg1	dynamics					1541:1548	surficial biomineralization dynamics	1513:1548	surficial biomineralization dynamics under the stress of OA and global warming	1513:1590	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	2	19	theme	ocean	630:634	arg1	OA					651:652	OA	651:652	OA	651:652	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	2	19	theme	ocean	630:634	arg1	acidification					636:648	ocean acidification	630:648	ocean acidification (OA)	630:653	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	2	20	theme	carbohydrate	431:442	arg1	composition					444:454	the carbohydrate composition	427:454	the carbohydrate composition of organic matrices across CCA taxa	427:490	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	7	21	theme	biomineralization	1523:1539	arg1	dynamics					1541:1548	surficial biomineralization dynamics	1513:1548	surficial biomineralization dynamics under the stress of OA and global warming	1513:1590	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	3	22	theme	mid-shelf	745:753	arg1	species					774:780	four mid-shelf Great Barrier Reef species	740:780	four mid-shelf Great Barrier Reef species of CCA	740:787	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	4	23	theme	surficial	1037:1045	arg1	tissue					1053:1058	surficial algal tissue	1037:1058	surficial algal tissue	1037:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	3	24	theme	IPCC	712:715	arg1	temperature					699:709	temperature	699:709	temperature (IPCC RCP 8.5)	699:724	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	3	24	theme	IPCC	712:715	arg1	RCP					717:719	IPCC RCP 8.5	712:723	IPCC RCP 8.5	712:723	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	1	25	theme	important	168:176	arg1	consolidators					196:208	the most important benthic substrate consolidators	159:208	the most important benthic substrate consolidators on coral reefs	159:223	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	0	26	theme	Cell	0:3	arg1	wall					5:8	Cell wall	0:8	Cell wall	0:8	Cell wall organic matrix composition and biomineralization across reef-building coralline algae under global change.
36301224	7	27	theme	OA	1570:1571	arg1	stress					1560:1565	the stress	1556:1565	the stress of OA and global warming	1556:1590	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	1	28	theme	benthic	178:184	arg1	consolidators					196:208	the most important benthic substrate consolidators	159:208	the most important benthic substrate consolidators on coral reefs	159:223	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	0	29	theme	matrix	18:23	arg1	composition					25:35	matrix composition	18:35	matrix composition	18:35	Cell wall organic matrix composition and biomineralization across reef-building coralline algae under global change.
36301224	2	30	theme	organic	545:551	arg1	constituents					560:571	their organic matrix constituents	539:571	their organic matrix constituents	539:571	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	2	31	theme	warming	659:665	arg1	threats					619:625	the threats	615:625	the threats of ocean acidification (OA) and warming	615:665	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	7	32	theme	monosaccharide	1461:1474	arg1	abundance					1476:1484	monosaccharide abundance	1461:1484	monosaccharide abundance	1461:1484	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	7	33	from	ability	1441:1447	arg1	composition					1425:1435	composition	1425:1435	composition	1425:1435	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	1	34	theme	substrate	186:194	arg1	consolidators					196:208	the most important benthic substrate consolidators	159:208	the most important benthic substrate consolidators on coral reefs	159:223	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	4	35	theme	algal	1047:1051	arg1	tissue					1053:1058	surficial algal tissue	1037:1058	surficial algal tissue	1037:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	1	36	theme	organic	282:288	arg1	matrix					290:295	an organic matrix	279:295	an organic matrix in their cell walls	279:315	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	4	37	theme	polysaccharides	992:1006	arg1	composition					973:983	the monosaccharide composition	954:983	the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue	954:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	1	38	theme	Crustose	117:124	arg1	CCA					143:145	CCA	143:145	CCA	143:145	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	1	38	theme	Crustose	117:124	arg1	algae					136:140	Crustose coralline algae	117:140	Crustose coralline algae (CCA)	117:146	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	1	39	theme	consolidators	196:208	arg1	consolidators					196:208	the most important benthic substrate consolidators	159:208	the most important benthic substrate consolidators on coral reefs	159:223	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	1	39	theme	consolidators	196:208	arg1	one					152:154	one	152:154	one	152:154	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	2	40	theme	matrices	467:474	arg1	composition					444:454	the carbohydrate composition	427:454	the carbohydrate composition of organic matrices across CCA taxa	427:490	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	3	41	theme	Barrier	761:767	arg1	Reef					769:772	Great Barrier Reef	755:772	four mid-shelf Great Barrier Reef species of CCA	740:787	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	4	42	theme	tissue	1053:1058	arg1	walls					1028:1032	the cell walls	1019:1032	the cell walls of surficial algal tissue	1019:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	1	43	theme	coral	213:217	arg1	reefs					219:223	coral reefs	213:223	coral reefs	213:223	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	2	44	theme	organic	459:465	arg1	matrices					467:474	organic matrices	459:474	organic matrices	459:474	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	3	45	theme	Reef	769:772	arg1	species					774:780	four mid-shelf Great Barrier Reef species	740:780	four mid-shelf Great Barrier Reef species of CCA	740:787	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	2	46	theme	environmental	580:592	arg1	change					594:599	environmental change	580:599	environmental change	580:599	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	2	47	from	role	374:377	arg1	biomineralization					382:398	biomineralization	382:398	biomineralization	382:398	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	1	48	theme	coralline	126:134	arg1	CCA					143:145	CCA	143:145	CCA	143:145	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	1	48	theme	coralline	126:134	arg1	algae					136:140	Crustose coralline algae	117:140	Crustose coralline algae (CCA)	117:146	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	4	49	theme	cell	1023:1026	arg1	walls					1028:1032	the cell walls	1019:1032	the cell walls of surficial algal tissue	1019:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	1	50	theme	cell	306:309	arg1	walls					311:315	their cell walls	300:315	their cell walls	300:315	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	7	51	theme	warming	1584:1590	arg1	stress					1560:1565	the stress	1556:1565	the stress of OA and global warming	1556:1590	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	1	52	from	matrix	290:295	arg1	walls					311:315	their cell walls	300:315	their cell walls	300:315	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	4	53	from	walls	1028:1032	arg1	present					1008:1014	present	1008:1014	present	1008:1014	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	3	54	theme	Great	755:759	arg1	Reef					769:772	Great Barrier Reef	755:772	four mid-shelf Great Barrier Reef species of CCA	740:787	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	1	55	from	consolidators	196:208	arg1	reefs					219:223	coral reefs	213:223	coral reefs	213:223	Crustose coralline algae (CCA) are one of the most important benthic substrate consolidators on coral reefs through their ability to deposit calcium carbonate on an organic matrix in their cell walls.
36301224	2	56	theme	Discrete	318:325	arg1	polysaccharides					327:341	Discrete polysaccharides	318:341	Discrete polysaccharides	318:341	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	3	57	theme	CCA	785:787	arg1	species					774:780	four mid-shelf Great Barrier Reef species	740:780	four mid-shelf Great Barrier Reef species of CCA	740:787	We simulated elevated pCO2 and temperature (IPCC RCP 8.5) and subjected four mid-shelf Great Barrier Reef species of CCA to 2 months of experimentation.
36301224	2	58	theme	matrix	553:558	arg1	constituents					560:571	their organic matrix constituents	539:571	their organic matrix constituents	539:571	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	0	59	theme	coralline	80:88	arg1	algae					90:94	reef-building coralline algae	66:94	reef-building coralline algae under global change	66:114	Cell wall organic matrix composition and biomineralization across reef-building coralline algae under global change.
36301224	7	60	theme	global	1577:1582	arg1	warming					1584:1590	global warming	1577:1590	global warming	1577:1590	Our findings suggest that both variability in composition and ability to modulate monosaccharide abundance may play a crucial role in surficial biomineralization dynamics under the stress of OA and global warming.
36301224	2	61	contain	have	509:512	arg2	capacity					518:525	the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming	514:665	the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming	514:665	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	2	61	contain	have	509:512	arg1	they					504:507	they	504:507	they	504:507	Discrete polysaccharides have been recognized for their role in biomineralization, yet little is known about the carbohydrate composition of organic matrices across CCA taxa and whether they have the capacity to modulate their organic matrix constituents amidst environmental change, particularly the threats of ocean acidification (OA) and warming.
36301224	0	62	theme	reef-building	66:78	arg1	algae					90:94	reef-building coralline algae	66:94	reef-building coralline algae under global change	66:114	Cell wall organic matrix composition and biomineralization across reef-building coralline algae under global change.
36301224	4	63	from	present	1008:1014	arg1	walls					1028:1032	the cell walls	1019:1032	the cell walls of surficial algal tissue	1019:1058	To assess the variability in surficial monosaccharide composition and biomineralization across species and treatments, we determined the monosaccharide composition of the polysaccharides present in the cell walls of surficial algal tissue and quantified calcification.
36301224	5	64	theme	organic	1189:1195	arg1	matrices					1197:1204	organic matrices	1189:1204	organic matrices	1189:1204	Our results revealed dissimilarity among species' monosaccharide constituents, which suggests that organic matrices are composed of different polysaccharides across CCA taxa.
35991887	2	0	theme	complex	415:421	arg1	composition					423:433	the mushroom's complex composition	400:433	the mushroom's complex composition	400:433	Because of the mushroom's complex composition, its pharmacological effects have not been fully clarified.
35991887	5	1	theme	gut	1041:1043	arg1	microbiota					1045:1054	gut microbiota	1041:1054	gut microbiota	1041:1054	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	6	2	theme	metabolomics	1193:1204	arg1	methods					1206:1212	metabolomics methods	1193:1212	metabolomics methods	1193:1212	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	8	3	theme	Chinese	1698:1704	arg1	medicine					1718:1725	Chinese traditional medicine	1698:1725	Chinese traditional medicine	1698:1725	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	5	4	theme	Lactobacillus	1118:1130	arg1	number					1108:1113	the number	1104:1113	the number of Lactobacillus (p < 0.01)	1104:1141	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	3	5	theme	fungal	623:628	arg1	mushroom					630:637	fungal mushroom	623:637	fungal mushroom	623:637	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	1	6	theme	diuretic	346:353	arg1	properties					377:386	its tranquilizing, diuretic, and spleen-enhancing properties	327:386	its tranquilizing, diuretic, and spleen-enhancing properties	327:386	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	7	7	theme	differing	1480:1488	arg1	effects					1490:1496	the differing effects	1476:1496	the differing effects of its three main metabolites on gut microbiota	1476:1544	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	3	8	theme	mushroom	630:637	arg1	value					614:618	potential medicinal value	594:618	potential medicinal value of fungal mushroom	594:637	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	1	9	theme	spleen-enhancing	360:375	arg1	properties					377:386	its tranquilizing, diuretic, and spleen-enhancing properties	327:386	its tranquilizing, diuretic, and spleen-enhancing properties	327:386	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	5	10	theme	p	1133:1133	arg1	Lactobacillus					1118:1130	Lactobacillus	1118:1130	Lactobacillus (p < 0.01)	1118:1141	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	5	10	theme	p	1133:1133	arg1	<					1135:1135	p < 0.01	1133:1140	p < 0.01	1133:1140	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	3	11	from	perspective	570:580	arg1	knowledge					520:528	our knowledge	516:528	our knowledge of these effects from a pharmacological perspective	516:580	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	11	from	perspective	570:580	arg1	effects					539:545	these effects	533:545	these effects from a pharmacological perspective	533:580	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	12	theme	main	659:662	arg1	saponins					794:801	triterpenoid saponins	781:801	triterpenoid saponins (PCZ)	781:807	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	12	theme	main	659:662	arg1	polysaccharides					715:729	water-soluble polysaccharides	701:729	water-soluble polysaccharides (PCX)	701:735	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	12	theme	main	659:662	arg1	polysaccharides					754:768	water-insoluble polysaccharides	738:768	water-insoluble polysaccharides (PCY)	738:774	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	12	theme	main	659:662	arg1	metabolites					664:674	three main metabolites	653:674	three main metabolites	653:674	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	2	13	theme	pharmacological	440:454	arg1	effects					456:462	its pharmacological effects	436:462	its pharmacological effects	436:462	Because of the mushroom's complex composition, its pharmacological effects have not been fully clarified.
35991887	5	14	theme	strongest	1010:1018	arg1	effect					1020:1025	the strongest effect	1006:1025	the strongest effect	1006:1025	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	6	15	theme	metabolites	1161:1171	arg1	total					1146:1150	A total	1144:1150	A total of 1,828 metabolites	1144:1171	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	0	16	dep	Gilb	69:72	arg1	regulate					74:81	regulate	74:81	regulate the gut microbiota in mice	74:108	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	6	17	theme	P.	1280:1281	arg1	cocos					1283:1287	P. cocos	1280:1287	P. cocos	1280:1287	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	6	18	theme	1,828	1155:1159	arg1	metabolites					1161:1171	1,828 metabolites	1155:1171	1,828 metabolites	1155:1171	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	4	19	from	effects	901:907	arg1	microbiota					927:936	the mice's gut microbiota	912:936	the mice's gut microbiota	912:936	These injections were made to explore the component's effects on the mice's gut microbiota and their metabolomics.
35991887	4	19	from	effects	901:907	arg1	metabolomics					948:959	their metabolomics	942:959	their metabolomics	942:959	These injections were made to explore the component's effects on the mice's gut microbiota and their metabolomics.
35991887	3	20	theme	intragastric	813:824	arg1	injection					826:834	intragastric injection	813:834	intragastric injection into mice	813:844	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	6	21	theme	main	1253:1256	arg1	metabolites					1265:1275	the three main active metabolites	1243:1275	the three main active metabolites of P. cocos	1243:1287	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	0	22	theme	main	6:9	arg1	metabolites					11:21	Three main metabolites	0:21	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.	0:160	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	3	23	from	cocos	684:688	arg1	saponins					794:801	triterpenoid saponins	781:801	triterpenoid saponins (PCZ)	781:807	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	23	from	cocos	684:688	arg1	polysaccharides					715:729	water-soluble polysaccharides	701:729	water-soluble polysaccharides (PCX)	701:735	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	23	from	cocos	684:688	arg1	polysaccharides					754:768	water-insoluble polysaccharides	738:768	water-insoluble polysaccharides (PCY)	738:774	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	23	from	cocos	684:688	arg1	metabolites					664:674	three main metabolites	653:674	three main metabolites	653:674	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	24	theme	potential	594:602	arg1	value					614:618	potential medicinal value	594:618	potential medicinal value of fungal mushroom	594:637	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	8	25	from	development	1657:1667	arg1	medicine					1718:1725	Chinese traditional medicine	1698:1725	Chinese traditional medicine	1698:1725	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	6	26	from	content	1315:1321	arg1	metabolites					1361:1371	intestinal metabolites	1350:1371	intestinal metabolites	1350:1371	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	7	27	theme	pharmacological	1424:1438	arg1	functions					1440:1448	the pharmacological functions	1420:1448	the pharmacological functions of P. cocos	1420:1460	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	1	28	theme	Wolfiporia	162:171	arg1	edible					249:254	edible	249:254	edible	249:254	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	1	28	theme	Wolfiporia	162:171	arg1	cocos					173:177	Wolfiporia cocos	162:177	Wolfiporia cocos	162:177	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	6	29	theme	peptides	1338:1345	arg1	content					1315:1321	the content	1311:1321	the content of short-chain peptides in intestinal metabolites	1311:1371	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	1	30	theme	Poria	223:227	arg1	cocos					229:233	Poria cocos	223:233	Poria cocos	223:233	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	8	31	theme	new	1625:1627	arg1	insights					1629:1636	new insights	1625:1636	new insights into the potential development of fungal polysaccharides in Chinese traditional medicine	1625:1725	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	6	32	theme	short-chain	1326:1336	arg1	peptides					1338:1345	short-chain peptides	1326:1345	short-chain peptides	1326:1345	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	0	33	theme	gut	87:89	arg1	microbiota					91:100	the gut microbiota	83:100	the gut microbiota in mice	83:108	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	7	34	theme	cocos	1456:1460	arg1	functions					1440:1448	the pharmacological functions	1420:1448	the pharmacological functions of P. cocos	1420:1460	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	3	35	from	knowledge	520:528	arg1	perspective					570:580	a pharmacological perspective	552:580	a pharmacological perspective	552:580	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	36	theme	P.	681:682	arg1	cocos					684:688	P. cocos	681:688	P. cocos	681:688	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	1	37	theme	years	317:321	arg1	thousands					304:312	thousands	304:312	thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties	304:386	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	6	38	theme	intestinal	1350:1359	arg1	metabolites					1361:1371	intestinal metabolites	1350:1371	intestinal metabolites	1350:1371	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	3	39	theme	pharmacological	554:568	arg1	perspective					570:580	a pharmacological perspective	552:580	a pharmacological perspective	552:580	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	7	40	theme	metabolites	1516:1526	arg1	effects					1490:1496	the differing effects	1476:1496	the differing effects of its three main metabolites on gut microbiota	1476:1544	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	4	41	theme	gut	923:925	arg1	microbiota					927:936	the mice's gut microbiota	912:936	the mice's gut microbiota	912:936	These injections were made to explore the component's effects on the mice's gut microbiota and their metabolomics.
35991887	7	42	theme	gut	1531:1533	arg1	microbiota					1535:1544	gut microbiota	1531:1544	gut microbiota	1531:1544	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	7	43	from	effects	1490:1496	arg1	microbiota					1535:1544	gut microbiota	1531:1544	gut microbiota	1531:1544	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	7	44	theme	P.	1453:1454	arg1	cocos					1456:1460	P. cocos	1453:1460	P. cocos	1453:1460	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	8	45	theme	potential	1647:1655	arg1	development					1657:1667	the potential development	1643:1667	the potential development of fungal polysaccharides in Chinese traditional medicine	1643:1725	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	7	46	theme	main	1511:1514	arg1	metabolites					1516:1526	three main metabolites	1505:1526	its three main metabolites	1501:1526	In conclusion, our study further investigated the pharmacological functions of P. cocos, and revealed the differing effects of its three main metabolites on gut microbiota.
35991887	1	47	theme	tranquilizing	331:343	arg1	properties					377:386	its tranquilizing, diuretic, and spleen-enhancing properties	327:386	its tranquilizing, diuretic, and spleen-enhancing properties	327:386	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	0	48	dep	Wolfiporia	28:37	arg1	cocos					39:43	cocos	39:43	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice	28:108	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	8	49	theme	fungal	1672:1677	arg1	polysaccharides					1679:1693	fungal polysaccharides	1672:1693	fungal polysaccharides	1672:1693	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	3	50	theme	triterpenoid	781:792	arg1	PCZ					804:806	PCZ	804:806	PCZ	804:806	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	50	theme	triterpenoid	781:792	arg1	saponins					794:801	triterpenoid saponins	781:801	triterpenoid saponins (PCZ)	781:807	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	51	theme	water-soluble	701:713	arg1	PCX					732:734	PCX	732:734	PCX	732:734	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	51	theme	water-soluble	701:713	arg1	polysaccharides					715:729	water-soluble polysaccharides	701:729	water-soluble polysaccharides (PCX)	701:735	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	0	52	theme	comparative	113:123	arg1	study					125:129	A comparative study	111:129	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.	0:160	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	0	53	from	Gilb	69:72	arg1	metabolites					11:21	Three main metabolites	0:21	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.	0:160	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	0	54	dep	metabolites	11:21	arg1	study					125:129	A comparative study	111:129	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.	0:160	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	5	55	theme	microbiota	966:975	arg1	analysis					977:984	The microbiota analysis	962:984	The microbiota analysis	962:984	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	3	56	theme	water-insoluble	738:752	arg1	PCY					771:773	PCY	771:773	PCY	771:773	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	56	theme	water-insoluble	738:752	arg1	polysaccharides					754:768	water-insoluble polysaccharides	738:768	water-insoluble polysaccharides (PCY)	738:774	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	3	57	theme	medicinal	604:612	arg1	value					614:618	potential medicinal value	594:618	potential medicinal value of fungal mushroom	594:637	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	1	58	dep	cocos	173:177	arg1	Wolf					186:189	Wolf	186:189	Wolf	186:189	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	1	58	dep	cocos	173:177	arg1	Gilb					203:206	Gilb	203:206	Gilb	203:206	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	8	59	theme	polysaccharides	1679:1693	arg1	development					1657:1667	the potential development	1643:1667	the potential development of fungal polysaccharides in Chinese traditional medicine	1643:1725	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	0	60	from	microbiota	91:100	arg1	mice					105:108	mice	105:108	mice	105:108	Three main metabolites from Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb regulate the gut microbiota in mice: A comparative study using microbiome-metabolomics.
35991887	6	61	theme	cocos	1283:1287	arg1	metabolites					1265:1275	the three main active metabolites	1243:1275	the three main active metabolites of P. cocos	1243:1287	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
35991887	1	62	theme	medicinal	260:268	arg1	mushroom					270:277	an ancient edible and medicinal mushroom	238:277	mushroom	270:277	Wolfiporia cocos (F. A. Wolf) Ryvarden & Gilb, also known as Poria cocos is an ancient edible and medicinal mushroom that has been valued for thousands of years for its tranquilizing, diuretic, and spleen-enhancing properties.
35991887	3	63	theme	effects	539:545	arg1	knowledge					520:528	our knowledge	516:528	our knowledge of these effects from a pharmacological perspective	516:580	Therefore, to expand our knowledge of these effects from a pharmacological perspective and exploit potential medicinal value of fungal mushroom, we extracted three main metabolites from P. cocos, including water-soluble polysaccharides (PCX), water-insoluble polysaccharides (PCY), and triterpenoid saponins (PCZ) for intragastric injection into mice.
35991887	8	64	theme	traditional	1706:1716	arg1	medicine					1718:1725	Chinese traditional medicine	1698:1725	Chinese traditional medicine	1698:1725	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	5	65	contain	had	1002:1004	arg1	PCY					998:1000	PCY	998:1000	PCY	998:1000	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	5	65	contain	had	1002:1004	arg2	effect					1020:1025	the strongest effect	1006:1025	the strongest effect	1006:1025	The microbiota analysis showed that PCY had the strongest effect on regulating gut microbiota through altering its composition and increasing the number of Lactobacillus (p < 0.01).
35991887	8	66	theme	prominent	1583:1591	arg1	prebiotic					1593:1601	a prominent prebiotic	1581:1601	a prominent prebiotic	1581:1601	The results suggested that PCY is a prominent prebiotic, and provided us with new insights into the potential development of fungal polysaccharides in Chinese traditional medicine.
35991887	6	67	theme	active	1258:1263	arg1	metabolites					1265:1275	the three main active metabolites	1243:1275	the three main active metabolites of P. cocos	1243:1287	A total of 1,828 metabolites were detected using metabolomics methods, and the results showed that the three main active metabolites of P. cocos significantly changed the content of short-chain peptides in intestinal metabolites.
37174446	5	0	theme	molecular	762:770	arg1	weight					772:777	the molecular weight	758:777	the molecular weight	758:777	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	3	1	dep	in	356:357	arg1	vitro					359:363	vitro	359:363	vitro	359:363	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	8	2	theme	catabolic	1508:1516	arg1	behavior					1518:1525	the catabolic behavior	1504:1525	the catabolic behavior of DP in the gastrointestinal tract	1504:1561	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
37174446	4	3	theme	sugar	576:580	arg1	content					582:588	the reducing sugar content	563:588	the reducing sugar content	563:588	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	0	4	theme	Microbial	74:82	arg1	Composition					84:94	Gut Microbial Composition	70:94	Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation	70:147	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	4	5	theme	molecular	622:630	arg1	weight					632:637	molecular weight	622:637	molecular weight	622:637	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	5	6	theme	fecal	899:903	arg1	fermentation					905:916	in vitro fecal fermentation	890:916	in vitro fecal fermentation	890:916	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	4	7	theme	reducing	567:574	arg1	content					582:588	the reducing sugar content	563:588	the reducing sugar content	563:588	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	0	8	theme	In	103:104	arg1	Digestion					112:120	In Vitro Digestion	103:120	In Vitro Digestion	103:120	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	0	9	from	Catabolism	0:9	arg1	Composition					84:94	Gut Microbial Composition	70:94	Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation	70:147	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	3	10	from	modulation	498:507	arg1	property					460:467	the potential catabolic property	436:467	the potential catabolic property of DP and its impacts on the modulation of gut microbial composition	436:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	7	11	theme	acid-base	1395:1403	arg1	environment					1405:1415	the acid-base environment	1391:1415	the acid-base environment of the large intestine	1391:1438	Moreover, DP could also promote the production of SCFAs, thus changing the acid-base environment of the large intestine.
37174446	6	12	theme	bacteria	1134:1141	arg1	abundances					1101:1110	the relative abundances	1088:1110	the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas,	1088:1212	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	3	13	theme	in	356:357	arg1	digestion					375:383	the in vitro simulated digestion	352:383	the in vitro simulated digestion	352:383	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	0	14	from	Impacts	59:65	arg1	Composition					84:94	Gut Microbial Composition	70:94	Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation	70:147	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	8	15	theme	microbiota-directed	1617:1635	arg1	products					1637:1644	microbiota-directed products	1617:1644	microbiota-directed products based on DP	1617:1656	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
37174446	7	16	theme	large	1424:1428	arg1	intestine					1430:1438	the large intestine	1420:1438	the large intestine	1420:1438	Moreover, DP could also promote the production of SCFAs, thus changing the acid-base environment of the large intestine.
37174446	5	17	theme	DP	850:851	arg1	viscosity					789:797	apparent viscosity	780:797	apparent viscosity	780:797	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	17	theme	DP	850:851	arg1	content					826:832	total polysaccharides content	804:832	total polysaccharides content of indigestible DP (DPI)	804:857	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	17	theme	DP	850:851	arg1	weight					772:777	the molecular weight	758:777	the molecular weight	758:777	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	4	18	theme	in	708:709	arg1	conditions					737:746	in vitro simulated digestive conditions	708:746	in vitro simulated digestive conditions	708:746	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	5	19	theme	sugar	936:940	arg1	content					942:948	the reducing sugar content	923:948	the reducing sugar content	923:948	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	3	20	theme	DP	472:473	arg1	property					460:467	the potential catabolic property	436:467	the potential catabolic property of DP and its impacts on the modulation of gut microbial composition	436:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	0	21	dep	In	103:104	arg1	Vitro					106:110	Vitro	106:110	Vitro	106:110	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	6	22	dep	composition	1275:1285	arg1	the					1271:1273	the	1271:1273	the	1271:1273	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	7	23	theme	intestine	1430:1438	arg1	environment					1405:1415	the acid-base environment	1391:1415	the acid-base environment of the large intestine	1391:1438	Moreover, DP could also promote the production of SCFAs, thus changing the acid-base environment of the large intestine.
37174446	3	24	theme	composition	526:536	arg1	modulation					498:507	the modulation	494:507	the modulation of gut microbial composition	494:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	3	25	theme	potential	440:448	arg1	property					460:467	the potential catabolic property	436:467	the potential catabolic property of DP and its impacts on the modulation of gut microbial composition	436:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	2	26	theme	beneficial	317:326	arg1	effects					328:334	its multiple beneficial effects	304:334	its multiple beneficial effects	304:334	D. indusiata polysaccharide (DP) has attracted increasing attention because of its multiple beneficial effects.
37174446	3	27	theme	microbial	516:524	arg1	composition					526:536	gut microbial composition	512:536	gut microbial composition	512:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	4	28	theme	DP	668:669	arg1	content					582:588	the reducing sugar content	563:588	the reducing sugar content	563:588	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	4	28	theme	DP	668:669	arg1	property					656:663	rheological property	644:663	rheological property	644:663	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	4	28	theme	DP	668:669	arg1	content					613:619	total polysaccharides content	591:619	total polysaccharides content	591:619	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	4	28	theme	DP	668:669	arg1	weight					632:637	molecular weight	622:637	molecular weight	622:637	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	5	29	theme	total	804:808	arg1	content					826:832	total polysaccharides content	804:832	total polysaccharides content of indigestible DP (DPI)	804:857	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	30	used	used	1050:1053	arg2	DP					1025:1026	DP	1025:1026	DP	1025:1026	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	31	theme	indigestible	837:848	arg1	DP					850:851	indigestible DP	837:851	indigestible DP (DPI)	837:857	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	31	theme	indigestible	837:848	arg1	DPI					854:856	DPI	854:856	DPI	854:856	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	3	32	from	impacts	483:489	arg1	modulation					498:507	the modulation	494:507	the modulation of gut microbial composition	494:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	2	33	theme	multiple	308:315	arg1	effects					328:334	its multiple beneficial effects	304:334	its multiple beneficial effects	304:334	D. indusiata polysaccharide (DP) has attracted increasing attention because of its multiple beneficial effects.
37174446	1	34	theme	famous	191:196	arg1	mushrooms					205:213	the most famous edible mushrooms	182:213	the most famous edible mushrooms in China	182:222	Dictyophora indusiata is one of the most famous edible mushrooms in China.
37174446	0	35	theme	indusiata	26:34	arg1	Polysaccharide					36:49	Dictyophora indusiata Polysaccharide	14:49	Dictyophora indusiata Polysaccharide	14:49	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	4	36	theme	digestive	727:735	arg1	conditions					737:746	in vitro simulated digestive conditions	708:746	in vitro simulated digestive conditions	708:746	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	0	37	theme	Microbial	126:134	arg1	Fermentation					136:147	Microbial Fermentation	126:147	Microbial Fermentation	126:147	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	4	38	dep	in	708:709	arg1	vitro					711:715	vitro	711:715	vitro	711:715	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	1	39	theme	edible	198:203	arg1	mushrooms					205:213	the most famous edible mushrooms	182:213	the most famous edible mushrooms in China	182:222	Dictyophora indusiata is one of the most famous edible mushrooms in China.
37174446	0	40	theme	Dictyophora	14:24	arg1	Polysaccharide					36:49	Dictyophora indusiata Polysaccharide	14:49	Dictyophora indusiata Polysaccharide	14:49	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	8	41	theme	gastrointestinal	1540:1555	arg1	tract					1557:1561	the gastrointestinal tract	1536:1561	the gastrointestinal tract	1536:1561	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
37174446	2	42	theme	increasing	272:281	arg1	attention					283:291	increasing attention	272:291	increasing attention	272:291	D. indusiata polysaccharide (DP) has attracted increasing attention because of its multiple beneficial effects.
37174446	8	43	from	behavior	1518:1525	arg1	tract					1557:1561	the gastrointestinal tract	1536:1561	the gastrointestinal tract	1536:1561	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
37174446	5	44	dep	in	890:891	arg1	vitro					893:897	vitro	893:897	vitro	893:897	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	45	theme	free	969:972	arg1	monosaccharides					974:988	free monosaccharides	969:988	free monosaccharides	969:988	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	1	46	theme	mushrooms	205:213	arg1	mushrooms					205:213	the most famous edible mushrooms	182:213	the most famous edible mushrooms in China	182:222	Dictyophora indusiata is one of the most famous edible mushrooms in China.
37174446	1	46	theme	mushrooms	205:213	arg1	one					175:177	one	175:177	one	175:177	Dictyophora indusiata is one of the most famous edible mushrooms in China.
37174446	5	47	theme	gut	1058:1060	arg1	microbiota					1062:1071	gut microbiota	1058:1071	gut microbiota	1058:1071	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	7	48	theme	SCFAs	1370:1374	arg1	production					1356:1365	the production	1352:1365	the production of SCFAs	1352:1374	Moreover, DP could also promote the production of SCFAs, thus changing the acid-base environment of the large intestine.
37174446	3	49	theme	catabolic	450:458	arg1	property					460:467	the potential catabolic property	436:467	the potential catabolic property of DP and its impacts on the modulation of gut microbial composition	436:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	0	50	theme	Polysaccharide	36:49	arg1	Catabolism					0:9	Catabolism	0:9	Catabolism of Dictyophora indusiata Polysaccharide	0:49	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	0	50	theme	Polysaccharide	36:49	arg1	Impacts					59:65	Its Impacts	55:65	Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation	55:147	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	5	51	theme	apparent	780:787	arg1	viscosity					789:797	apparent viscosity	780:797	apparent viscosity	780:797	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	2	52	theme	indusiata	228:236	arg1	DP					254:255	DP	254:255	DP	254:255	D. indusiata polysaccharide (DP) has attracted increasing attention because of its multiple beneficial effects.
37174446	2	52	theme	indusiata	228:236	arg1	polysaccharide					238:251	D. indusiata polysaccharide	225:251	D. indusiata polysaccharide (DP)	225:256	D. indusiata polysaccharide (DP) has attracted increasing attention because of its multiple beneficial effects.
37174446	5	53	theme	polysaccharides	810:824	arg1	content					826:832	total polysaccharides content	804:832	total polysaccharides content of indigestible DP (DPI)	804:857	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	3	54	theme	microbial	389:397	arg1	fermentation					399:410	microbial fermentation	389:410	microbial fermentation	389:410	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	6	55	theme	microbiota	1308:1317	arg1	abundance					1291:1299	abundance	1291:1299	abundance	1291:1299	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	55	theme	microbiota	1308:1317	arg1	composition					1275:1285	composition	1275:1285	composition	1275:1285	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	8	56	theme	DP	1530:1531	arg1	behavior					1518:1525	the catabolic behavior	1504:1525	the catabolic behavior of DP in the gastrointestinal tract	1504:1561	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
37174446	2	57	theme	D.	225:226	arg1	DP					254:255	DP	254:255	DP	254:255	D. indusiata polysaccharide (DP) has attracted increasing attention because of its multiple beneficial effects.
37174446	2	57	theme	D.	225:226	arg1	polysaccharide					238:251	D. indusiata polysaccharide	225:251	D. indusiata polysaccharide (DP)	225:256	D. indusiata polysaccharide (DP) has attracted increasing attention because of its multiple beneficial effects.
37174446	3	58	from	property	460:467	arg1	modulation					498:507	the modulation	494:507	the modulation of gut microbial composition	494:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	3	59	theme	impacts	483:489	arg1	property					460:467	the potential catabolic property	436:467	the potential catabolic property of DP and its impacts on the modulation of gut microbial composition	436:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	6	60	theme	gut	1304:1306	arg1	microbiota					1308:1317	gut microbiota	1304:1317	gut microbiota	1304:1317	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	61	theme	several	1115:1121	arg1	bacteria					1134:1141	several beneficial bacteria	1115:1141	several beneficial bacteria	1115:1141	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	61	theme	several	1115:1121	arg1	Megamonas					1203:1211	Megamonas	1203:1211	Megamonas	1203:1211	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	61	theme	several	1115:1121	arg1	Catenibacterium					1165:1179	Catenibacterium	1165:1179	Catenibacterium	1165:1179	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	61	theme	several	1115:1121	arg1	Parabacteroides					1182:1196	Parabacteroides	1182:1196	Parabacteroides	1182:1196	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	5	62	theme	reducing	927:934	arg1	content					942:948	the reducing sugar content	923:948	the reducing sugar content	923:948	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	63	theme	monosaccharides	974:988	arg1	content					942:948	the reducing sugar content	923:948	the reducing sugar content	923:948	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	5	63	theme	monosaccharides	974:988	arg1	release					958:964	the release	954:964	the release of free monosaccharides	954:988	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	1	64	from	mushrooms	205:213	arg1	China					218:222	China	218:222	China	218:222	Dictyophora indusiata is one of the most famous edible mushrooms in China.
37174446	4	65	theme	simulated	717:725	arg1	conditions					737:746	in vitro simulated digestive conditions	708:746	in vitro simulated digestive conditions	708:746	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	4	66	theme	polysaccharides	597:611	arg1	content					613:619	total polysaccharides content	591:619	total polysaccharides content	591:619	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	3	67	from	DP	472:473	arg1	modulation					498:507	the modulation	494:507	the modulation of gut microbial composition	494:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	5	68	theme	in	890:891	arg1	fermentation					905:916	in vitro fecal fermentation	890:916	in vitro fecal fermentation	890:916	However, the molecular weight, apparent viscosity, and total polysaccharides content of indigestible DP (DPI) significantly decreased during in vitro fecal fermentation, and the reducing sugar content and the release of free monosaccharides notably increased, suggesting that DP could be degraded and used by gut microbiota.
37174446	6	69	theme	beneficial	1123:1132	arg1	bacteria					1134:1141	several beneficial bacteria	1115:1141	several beneficial bacteria	1115:1141	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	69	theme	beneficial	1123:1132	arg1	Megamonas					1203:1211	Megamonas	1203:1211	Megamonas	1203:1211	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	69	theme	beneficial	1123:1132	arg1	Catenibacterium					1165:1179	Catenibacterium	1165:1179	Catenibacterium	1165:1179	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	6	69	theme	beneficial	1123:1132	arg1	Parabacteroides					1182:1196	Parabacteroides	1182:1196	Parabacteroides	1182:1196	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	4	70	theme	total	591:595	arg1	content					613:619	total polysaccharides content	591:619	total polysaccharides content	591:619	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	0	71	theme	Gut	70:72	arg1	Composition					84:94	Gut Microbial Composition	70:94	Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation	70:147	Catabolism of Dictyophora indusiata Polysaccharide and Its Impacts on Gut Microbial Composition during In Vitro Digestion and Microbial Fermentation.
37174446	8	72	theme	study	1461:1465	arg1	beneficial					1471:1480	beneficial	1471:1480	beneficial	1471:1480	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
37174446	8	72	theme	study	1461:1465	arg1	results					1445:1451	The results	1441:1451	The results of this study	1441:1465	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
37174446	1	73	theme	Dictyophora	150:160	arg1	indusiata					162:170	Dictyophora indusiata	150:170	Dictyophora indusiata	150:170	Dictyophora indusiata is one of the most famous edible mushrooms in China.
37174446	4	74	theme	rheological	644:654	arg1	property					656:663	rheological property	644:663	rheological property	644:663	The results showed that the reducing sugar content, total polysaccharides content, molecular weight, and rheological property of DP were not significantly altered under in vitro simulated digestive conditions.
37174446	6	75	theme	relative	1092:1099	arg1	abundances					1101:1110	the relative abundances	1088:1110	the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas,	1088:1212	Additionally, the relative abundances of several beneficial bacteria, such as Bacteroides, Catenibacterium, Parabacteroides, and Megamonas, increased significantly, indicating that DP can regulate the composition and abundance of gut microbiota.
37174446	3	76	theme	simulated	365:373	arg1	digestion					375:383	the in vitro simulated digestion	352:383	the in vitro simulated digestion	352:383	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	3	77	theme	gut	512:514	arg1	composition					526:536	gut microbial composition	512:536	gut microbial composition	512:536	In this study, the in vitro simulated digestion and microbial fermentation were designed to reveal the potential catabolic property of DP and its impacts on the modulation of gut microbial composition.
37174446	8	78	theme	theoretical	1584:1594	arg1	basis					1596:1600	a theoretical basis	1582:1600	a theoretical basis for developing microbiota-directed products based on DP	1582:1656	The results of this study are beneficial for deeply clarifying the catabolic behavior of DP in the gastrointestinal tract, which can provide a theoretical basis for developing microbiota-directed products based on DP.
36726569	12	0	theme	hexavalent	1813:1822	arg1	exposure					1833:1840	hexavalent chromium exposure	1813:1840	hexavalent chromium exposure	1813:1840	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	11	1	theme	seaweed	1624:1630	arg1	administration					1648:1661	seaweed polysaccharides administration	1624:1661	seaweed polysaccharides administration	1624:1661	However, seaweed polysaccharides administration could ameliorate the composition of gut microbiota.
36726569	2	2	theme	food	328:331	arg1	chain					333:337	the food chain	324:337	the food chain	324:337	Additionally, humans and other mammals may also be directly exposed to heavy metals or exposed to heavy metals through the food chain, which seriously threatens the health of animals and humans.
36726569	8	3	theme	experiment	1131:1140	arg1	end					1120:1122	the end	1116:1122	the end of the experiment	1116:1140	At the end of the experiment, all mice were euthanized, and colon contents were collected for DNA sequencing analysis.
36726569	1	4	theme	food	174:177	arg1	safety					179:184	food safety	174:184	food safety	174:184	Heavy metals released in the environment pose a huge threat to soil and water quality, food safety and public health.
36726569	8	5	theme	sequencing	1211:1220	arg1	analysis					1222:1229	DNA sequencing analysis	1207:1229	DNA sequencing analysis	1207:1229	At the end of the experiment, all mice were euthanized, and colon contents were collected for DNA sequencing analysis.
36726569	7	6	theme	treatment	1063:1071	arg1	group					1073:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Mice in Cr and seaweed polysaccharide treatment group was compulsively receive K2Cr2O7.
36726569	13	7	theme	polysaccharides	1967:1981	arg1	application					1944:1954	the application	1940:1954	the application of seaweed polysaccharides	1940:1981	Meanwhile, this research also lays the foundation for the application of seaweed polysaccharides.
36726569	3	8	theme	serious	501:507	arg1	pollution					523:531	serious environmental pollution	501:531	serious environmental pollution as well as intestinal damage	501:560	Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage.
36726569	12	9	theme	seaweed	1753:1759	arg1	polysaccharides					1761:1775	seaweed polysaccharides	1753:1775	seaweed polysaccharides	1753:1775	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	7	10	theme	seaweed	1040:1046	arg1	group					1073:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Mice in Cr and seaweed polysaccharide treatment group was compulsively receive K2Cr2O7.
36726569	8	11	theme	colon	1173:1177	arg1	contents					1179:1186	colon contents	1173:1186	colon contents	1173:1186	At the end of the experiment, all mice were euthanized, and colon contents were collected for DNA sequencing analysis.
36726569	4	12	theme	negative	633:640	arg1	effects					649:655	the negative health effects	629:655	the negative health effects of hexavalent chromium exposure	629:687	Thus, increasing research is devoted to finding drugs to mitigate the negative health effects of hexavalent chromium exposure.
36726569	7	13	theme	Cr	1033:1034	arg1	group					1073:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Mice in Cr and seaweed polysaccharide treatment group was compulsively receive K2Cr2O7.
36726569	4	14	theme	health	642:647	arg1	effects					649:655	the negative health effects	629:655	the negative health effects of hexavalent chromium exposure	629:687	Thus, increasing research is devoted to finding drugs to mitigate the negative health effects of hexavalent chromium exposure.
36726569	1	15	theme	huge	135:138	arg1	threat					140:145	a huge threat	133:145	a huge threat to soil and water quality, food safety and public health	133:202	Heavy metals released in the environment pose a huge threat to soil and water quality, food safety and public health.
36726569	5	16	contain	have	740:743	arg1	polysaccharides					698:712	Seaweed polysaccharides	690:712	Seaweed polysaccharides	690:712	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	5	16	contain	have	740:743	arg2	effects					766:772	many pharmacological effects	745:772	many pharmacological effects	745:772	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	6	17	theme	poor	1004:1007	arg1	health					1009:1014	hexavalent chromium exposure-induced poor health	967:1014	hexavalent chromium exposure-induced poor health in mice	967:1022	Here, we hypothesized that seaweed polysaccharides could alleviate hexavalent chromium exposure-induced poor health in mice.
36726569	3	18	theme	intestinal	544:553	arg1	damage					555:560	intestinal damage	544:560	serious environmental pollution as well as intestinal damage	501:560	Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage.
36726569	5	19	theme	hexavalent	838:847	arg1	exposure					858:865	hexavalent chromium exposure	838:865	hexavalent chromium exposure	838:865	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	6	20	theme	chromium	978:985	arg1	health					1009:1014	hexavalent chromium exposure-induced poor health	967:1014	hexavalent chromium exposure-induced poor health in mice	967:1022	Here, we hypothesized that seaweed polysaccharides could alleviate hexavalent chromium exposure-induced poor health in mice.
36726569	9	21	theme	polysaccharide	1260:1273	arg1	administration					1275:1288	seaweed polysaccharide administration	1252:1288	seaweed polysaccharide administration	1252:1288	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	9	22	dep	dysbiosis	1320:1328	arg1	the					1302:1304	the	1302:1304	the	1302:1304	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	6	23	from	health	1009:1014	arg1	mice					1019:1022	mice	1019:1022	mice	1019:1022	Here, we hypothesized that seaweed polysaccharides could alleviate hexavalent chromium exposure-induced poor health in mice.
36726569	1	24	theme	Heavy	87:91	arg1	metals					93:98	Heavy metals	87:98	Heavy metals released in the environment	87:126	Heavy metals released in the environment pose a huge threat to soil and water quality, food safety and public health.
36726569	4	25	theme	chromium	671:678	arg1	exposure					680:687	hexavalent chromium exposure	660:687	hexavalent chromium exposure	660:687	Thus, increasing research is devoted to finding drugs to mitigate the negative health effects of hexavalent chromium exposure.
36726569	0	26	theme	Seaweed	0:6	arg1	polysaccharide					8:21	Seaweed polysaccharide	0:21	Seaweed polysaccharide	0:21	Seaweed polysaccharide relieves hexavalent chromium-induced gut microbial homeostasis.
36726569	9	27	theme	microbial	1310:1318	arg1	dysbiosis					1320:1328	gut microbial dysbiosis	1306:1328	gut microbial dysbiosis	1306:1328	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	10	28	theme	microbial	1495:1503	arg1	composition					1505:1515	the gut microbial composition	1487:1515	the gut microbial composition of mice	1487:1523	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	1	29	dep	soil	150:153	arg1	quality					165:171	quality	165:171	quality	165:171	Heavy metals released in the environment pose a huge threat to soil and water quality, food safety and public health.
36726569	2	30	theme	humans	392:397	arg1	health					370:375	the health	366:375	the health of animals and humans	366:397	Additionally, humans and other mammals may also be directly exposed to heavy metals or exposed to heavy metals through the food chain, which seriously threatens the health of animals and humans.
36726569	2	31	theme	animals	380:386	arg1	health					370:375	the health	366:375	the health of animals and humans	366:397	Additionally, humans and other mammals may also be directly exposed to heavy metals or exposed to heavy metals through the food chain, which seriously threatens the health of animals and humans.
36726569	3	32	theme	hexavalent	421:430	arg1	chromium					432:439	hexavalent chromium	421:439	hexavalent chromium	421:439	Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage.
36726569	0	33	theme	chromium-induced	43:58	arg1	homeostasis					74:84	hexavalent chromium-induced gut microbial homeostasis	32:84	hexavalent chromium-induced gut microbial homeostasis	32:84	Seaweed polysaccharide relieves hexavalent chromium-induced gut microbial homeostasis.
36726569	9	34	from	exposure	1405:1412	arg1	mice					1417:1420	mice	1417:1420	mice	1417:1420	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	3	35	dep	Chromium	400:407	arg1	chromium					432:439	hexavalent chromium	421:439	hexavalent chromium	421:439	Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage.
36726569	3	35	dep	Chromium	400:407	arg1	VI					446:447	VI	446:447	VI	446:447	Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage.
36726569	3	35	dep	Chromium	400:407	arg1	[Cr					441:443	[Cr	441:443	[Cr	441:443	Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage.
36726569	11	36	theme	microbiota	1703:1712	arg1	composition					1684:1694	the composition	1680:1694	the composition of gut microbiota	1680:1712	However, seaweed polysaccharides administration could ameliorate the composition of gut microbiota.
36726569	5	37	theme	many	745:748	arg1	effects					766:772	many pharmacological effects	745:772	many pharmacological effects	745:772	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	9	38	theme	gut	1351:1353	arg1	diversity					1365:1373	gut microbial diversity	1351:1373	gut microbial diversity	1351:1373	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	9	39	theme	diversity	1365:1373	arg1	dysbiosis					1320:1328	gut microbial dysbiosis	1306:1328	gut microbial dysbiosis	1306:1328	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	9	39	theme	diversity	1365:1373	arg1	reduction					1338:1346	the reduction	1334:1346	the reduction of gut microbial diversity	1334:1373	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	12	40	theme	microbial	1865:1873	arg1	dysbiosis					1875:1883	gut microbial dysbiosis	1861:1883	gut microbial dysbiosis	1861:1883	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	5	41	theme	microbial	808:816	arg1	dysbiosis					818:826	gut microbial dysbiosis	804:826	gut microbial dysbiosis caused by hexavalent chromium exposure	804:865	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	10	42	from	decrease	1582:1589	arg1	bacteria					1567:1574	some pathogenic bacteria	1551:1574	some pathogenic bacteria	1551:1574	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	10	42	from	decrease	1582:1589	arg1	bacteria					1605:1612	beneficial bacteria	1594:1612	beneficial bacteria	1594:1612	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	12	43	theme	chromium	1824:1831	arg1	exposure					1833:1840	hexavalent chromium exposure	1813:1840	hexavalent chromium exposure	1813:1840	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	7	44	from	Mice	1025:1028	arg1	group					1073:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Mice in Cr and seaweed polysaccharide treatment group was compulsively receive K2Cr2O7.
36726569	9	45	theme	chromium	1396:1403	arg1	exposure					1405:1412	hexavalent chromium exposure	1385:1412	hexavalent chromium exposure in mice	1385:1420	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	12	46	from	effects	1802:1808	arg1	dysbiosis					1875:1883	gut microbial dysbiosis	1861:1883	gut microbial dysbiosis	1861:1883	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	12	46	from	effects	1802:1808	arg1	mice					1845:1848	mice	1845:1848	mice	1845:1848	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	11	47	theme	polysaccharides	1632:1646	arg1	administration					1648:1661	seaweed polysaccharides administration	1624:1661	seaweed polysaccharides administration	1624:1661	However, seaweed polysaccharides administration could ameliorate the composition of gut microbiota.
36726569	2	48	theme	heavy	276:280	arg1	metals					282:287	heavy metals	276:287	heavy metals	276:287	Additionally, humans and other mammals may also be directly exposed to heavy metals or exposed to heavy metals through the food chain, which seriously threatens the health of animals and humans.
36726569	12	49	theme	negative	1793:1800	arg1	effects					1802:1808	the negative effects	1789:1808	the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis	1789:1883	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	10	50	theme	pathogenic	1556:1565	arg1	bacteria					1567:1574	some pathogenic bacteria	1551:1574	some pathogenic bacteria	1551:1574	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	5	51	theme	gut	804:806	arg1	dysbiosis					818:826	gut microbial dysbiosis	804:826	gut microbial dysbiosis caused by hexavalent chromium exposure	804:865	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	0	52	theme	microbial	64:72	arg1	homeostasis					74:84	hexavalent chromium-induced gut microbial homeostasis	32:84	hexavalent chromium-induced gut microbial homeostasis	32:84	Seaweed polysaccharide relieves hexavalent chromium-induced gut microbial homeostasis.
36726569	10	53	theme	beneficial	1594:1603	arg1	bacteria					1605:1612	beneficial bacteria	1594:1612	beneficial bacteria	1594:1612	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	7	54	theme	polysaccharide	1048:1061	arg1	group					1073:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Cr and seaweed polysaccharide treatment group	1033:1077	Mice in Cr and seaweed polysaccharide treatment group was compulsively receive K2Cr2O7.
36726569	13	55	theme	seaweed	1959:1965	arg1	polysaccharides					1967:1981	seaweed polysaccharides	1959:1981	seaweed polysaccharides	1959:1981	Meanwhile, this research also lays the foundation for the application of seaweed polysaccharides.
36726569	6	56	theme	seaweed	927:933	arg1	polysaccharides					935:949	seaweed polysaccharides	927:949	seaweed polysaccharides	927:949	Here, we hypothesized that seaweed polysaccharides could alleviate hexavalent chromium exposure-induced poor health in mice.
36726569	8	57	theme	DNA	1207:1209	arg1	analysis					1222:1229	DNA sequencing analysis	1207:1229	DNA sequencing analysis	1207:1229	At the end of the experiment, all mice were euthanized, and colon contents were collected for DNA sequencing analysis.
36726569	1	58	theme	public	190:195	arg1	health					197:202	public health	190:202	public health	190:202	Heavy metals released in the environment pose a huge threat to soil and water quality, food safety and public health.
36726569	2	59	theme	other	230:234	arg1	mammals					236:242	other mammals	230:242	other mammals	230:242	Additionally, humans and other mammals may also be directly exposed to heavy metals or exposed to heavy metals through the food chain, which seriously threatens the health of animals and humans.
36726569	4	60	theme	increasing	569:578	arg1	research					580:587	increasing research	569:587	increasing research	569:587	Thus, increasing research is devoted to finding drugs to mitigate the negative health effects of hexavalent chromium exposure.
36726569	3	61	theme	environmental	509:521	arg1	pollution					523:531	serious environmental pollution	501:531	serious environmental pollution as well as intestinal damage	501:560	Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage.
36726569	10	62	from	changes	1476:1482	arg1	composition					1505:1515	the gut microbial composition	1487:1515	the gut microbial composition of mice	1487:1523	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	10	63	theme	Hexavalent	1423:1432	arg1	exposure					1443:1450	Hexavalent chromium exposure	1423:1450	Hexavalent chromium exposure	1423:1450	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	5	64	theme	Seaweed	690:696	arg1	polysaccharides					698:712	Seaweed polysaccharides	690:712	Seaweed polysaccharides	690:712	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	6	65	theme	exposure-induced	987:1002	arg1	health					1009:1014	hexavalent chromium exposure-induced poor health	967:1014	hexavalent chromium exposure-induced poor health in mice	967:1022	Here, we hypothesized that seaweed polysaccharides could alleviate hexavalent chromium exposure-induced poor health in mice.
36726569	9	66	theme	seaweed	1252:1258	arg1	administration					1275:1288	seaweed polysaccharide administration	1252:1288	seaweed polysaccharide administration	1252:1288	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	5	67	theme	chromium	849:856	arg1	exposure					858:865	hexavalent chromium exposure	838:865	hexavalent chromium exposure	838:865	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	6	68	theme	hexavalent	967:976	arg1	health					1009:1014	hexavalent chromium exposure-induced poor health	967:1014	hexavalent chromium exposure-induced poor health in mice	967:1022	Here, we hypothesized that seaweed polysaccharides could alleviate hexavalent chromium exposure-induced poor health in mice.
36726569	10	69	theme	chromium	1434:1441	arg1	exposure					1443:1450	Hexavalent chromium exposure	1423:1450	Hexavalent chromium exposure	1423:1450	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	10	70	theme	significant	1464:1474	arg1	increase					1539:1546	an increase	1536:1546	an increase in some pathogenic bacteria	1536:1574	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	10	70	theme	significant	1464:1474	arg1	decrease					1582:1589	a decrease	1580:1589	a decrease in beneficial bacteria	1580:1612	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	10	70	theme	significant	1464:1474	arg1	changes					1476:1482	significant changes	1464:1482	significant changes	1464:1482	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	5	71	theme	pharmacological	750:764	arg1	effects					766:772	many pharmacological effects	745:772	many pharmacological effects	745:772	Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized.
36726569	9	72	theme	gut	1306:1308	arg1	dysbiosis					1320:1328	gut microbial dysbiosis	1306:1328	gut microbial dysbiosis	1306:1328	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	4	73	theme	hexavalent	660:669	arg1	exposure					680:687	hexavalent chromium exposure	660:687	hexavalent chromium exposure	660:687	Thus, increasing research is devoted to finding drugs to mitigate the negative health effects of hexavalent chromium exposure.
36726569	0	74	theme	hexavalent	32:41	arg1	homeostasis					74:84	hexavalent chromium-induced gut microbial homeostasis	32:84	hexavalent chromium-induced gut microbial homeostasis	32:84	Seaweed polysaccharide relieves hexavalent chromium-induced gut microbial homeostasis.
36726569	10	75	theme	gut	1491:1493	arg1	composition					1505:1515	the gut microbial composition	1487:1515	the gut microbial composition of mice	1487:1523	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	4	76	theme	exposure	680:687	arg1	effects					649:655	the negative health effects	629:655	the negative health effects of hexavalent chromium exposure	629:687	Thus, increasing research is devoted to finding drugs to mitigate the negative health effects of hexavalent chromium exposure.
36726569	0	77	theme	gut	60:62	arg1	homeostasis					74:84	hexavalent chromium-induced gut microbial homeostasis	32:84	hexavalent chromium-induced gut microbial homeostasis	32:84	Seaweed polysaccharide relieves hexavalent chromium-induced gut microbial homeostasis.
36726569	11	78	theme	gut	1699:1701	arg1	microbiota					1703:1712	gut microbiota	1699:1712	gut microbiota	1699:1712	However, seaweed polysaccharides administration could ameliorate the composition of gut microbiota.
36726569	12	79	theme	gut	1861:1863	arg1	dysbiosis					1875:1883	gut microbial dysbiosis	1861:1883	gut microbial dysbiosis	1861:1883	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	10	80	from	increase	1539:1546	arg1	bacteria					1567:1574	some pathogenic bacteria	1551:1574	some pathogenic bacteria	1551:1574	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	10	80	from	increase	1539:1546	arg1	bacteria					1605:1612	beneficial bacteria	1594:1612	beneficial bacteria	1594:1612	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	12	81	theme	exposure	1833:1840	arg1	effects					1802:1808	the negative effects	1789:1808	the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis	1789:1883	In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis.
36726569	10	82	theme	mice	1520:1523	arg1	composition					1505:1515	the gut microbial composition	1487:1515	the gut microbial composition of mice	1487:1523	Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria.
36726569	9	83	theme	microbial	1355:1363	arg1	diversity					1365:1373	gut microbial diversity	1351:1373	gut microbial diversity	1351:1373	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
36726569	2	84	theme	heavy	303:307	arg1	metals					309:314	heavy metals	303:314	heavy metals	303:314	Additionally, humans and other mammals may also be directly exposed to heavy metals or exposed to heavy metals through the food chain, which seriously threatens the health of animals and humans.
36726569	9	85	theme	hexavalent	1385:1394	arg1	exposure					1405:1412	hexavalent chromium exposure	1385:1412	hexavalent chromium exposure in mice	1385:1420	Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice.
35202713	15	0	from	decreases	2436:2444	arg1	deposition					2472:2481	peribronchial collagen deposition	2449:2481	peribronchial collagen deposition	2449:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	6	1	theme	treated	1092:1098	arg1	groups					1100:1105	three MS treated groups	1083:1105	three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg	1083:1140	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	1	theme	treated	1092:1098	arg1	13 mg/kg					1119:1126	13 mg/kg	1119:1126	13 mg/kg	1119:1126	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	1	theme	treated	1092:1098	arg1	39 mg/kg					1133:1140	39 mg/kg	1133:1140	39 mg/kg	1133:1140	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	1	theme	treated	1092:1098	arg1	4.3 mg/kg					1108:1116	4.3 mg/kg	1108:1116	4.3 mg/kg	1108:1116	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	3	2	theme	molecular	710:718	arg1	intermediates					720:732	the relevant cellular, biochemical and molecular intermediates	671:732	the relevant cellular, biochemical and molecular intermediates	671:732	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	3	3	theme	anti-asthmatic	521:534	arg1	properties					536:545	the anti-inflammatory and anti-asthmatic properties	495:545	the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS	495:581	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	3	4	theme	MS	580:581	arg1	properties					536:545	the anti-inflammatory and anti-asthmatic properties	495:545	the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS	495:581	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	8	5	from	heterogenous	1399:1410	arg1	shape					1415:1419	shape	1415:1419	shape	1415:1419	RESULTS SEM image analysis showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm.
35202713	9	6	theme	sulfide	1615:1621	arg1	forms					1598:1602	the forms	1594:1602	the forms of mercury sulfide, arsenic trioxide, and their alloy crystals	1594:1665	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	11	7	theme	MS-exhibited	1855:1866	arg1	alleviation					1868:1878	MS-exhibited alleviation	1855:1878	MS-exhibited alleviation of allergic asthma features	1855:1906	Mice receiving MS-exhibited alleviation of allergic asthma features.
35202713	2	8	theme	Malla	290:294	arg1	formulation					342:352	a metal-based ethnomedicinal formulation	313:352	a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations	313:456	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	2	8	theme	Malla	290:294	arg1	Sindoor					296:302	'Malla Sindoor'	289:303	'Malla Sindoor' (MS)	289:308	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	2	8	theme	Malla	290:294	arg1	MS					306:307	MS	306:307	MS	306:307	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	9	9	theme	arsenic	1624:1630	arg1	trioxide					1632:1639	arsenic trioxide	1624:1639	arsenic trioxide	1624:1639	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	8	10	with	heterogenous	1399:1410	arg1	distribution					1442:1453	a particle size distribution	1426:1453	a particle size distribution between 100 nm-1 μm	1426:1473	RESULTS SEM image analysis showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm.
35202713	15	11	theme	cell	2552:2555	arg1	infiltration					2557:2568	inflammatory cell infiltration	2539:2568	inflammatory cell infiltration	2539:2568	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	12	12	theme	total	1953:1957	arg1	count					1964:1968	the total cell count	1949:1968	the total cell count	1949:1968	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	15	13	from	reduction	2392:2400	arg1	decreases					2436:2444	decreases	2436:2444	decreases in peribronchial collagen deposition	2436:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	15	13	from	reduction	2392:2400	arg1	attenuation					2524:2534	attenuation	2524:2534	attenuation of inflammatory cell infiltration	2524:2568	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	15	13	from	reduction	2392:2400	arg1	airways					2427:2433	airways	2427:2433	airways	2427:2433	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	15	13	from	reduction	2392:2400	arg1	thickening					2508:2517	bronchial smooth muscle thickening	2484:2517	bronchial smooth muscle thickening	2484:2517	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	15	13	from	reduction	2392:2400	arg1	accumulation					2411:2422	mucus accumulation	2405:2422	mucus accumulation	2405:2422	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	13	14	theme	interleukin	2075:2085	arg1	IL					2088:2089	interleukin (IL)-1β	2075:2093	interleukin (IL)-1β	2075:2093	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	3	15	theme	asthmatic	586:594	arg1	model					601:605	asthmatic mice model	586:605	asthmatic mice model	586:605	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	1	16	theme	inflammatory	216:227	arg1	disease					229:235	the leading inflammatory disease	204:235	the leading inflammatory disease of the airways with inadequate therapeutic options	204:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	1	16	theme	inflammatory	216:227	arg1	Asthma					194:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	8	17	theme	particle	1428:1435	arg1	distribution					1442:1453	a particle size distribution	1426:1453	a particle size distribution between 100 nm-1 μm	1426:1473	RESULTS SEM image analysis showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm.
35202713	13	18	theme	IL	2088:2089	arg1	levels					2065:2070	the stimulated levels	2050:2070	the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines	2050:2118	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	13	18	theme	IL	2088:2089	arg1	levels					2136:2141	antioxidant levels	2124:2141	antioxidant levels	2124:2141	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	3	19	theme	stimulated	618:627	arg1	THP-1 cells					645:655	LPS stimulated human monocytic THP-1 cells	614:655	LPS stimulated human monocytic THP-1 cells	614:655	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	15	20	theme	mucus	2405:2409	arg1	accumulation					2411:2422	mucus accumulation	2405:2422	mucus accumulation	2405:2422	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	9	21	theme	crystals	1658:1665	arg1	forms					1598:1602	the forms	1594:1602	the forms of mercury sulfide, arsenic trioxide, and their alloy crystals	1594:1665	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	0	22	from	inflammation	62:73	arg1	model					85:89	murine model	78:89	murine model of allergic asthma	78:108	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	0	23	theme	oxidative	145:153	arg1	stress					155:160	oxidative stress	145:160	oxidative stress	145:160	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	0	23	theme	oxidative	145:153	arg1	cytokines					124:132	cytokines	124:132	cytokines status and oxidative stress	124:160	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	10	24	theme	overt	1732:1736	arg1	features					1751:1758	overt pathological features	1732:1758	overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition	1732:1837	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	10	24	theme	overt	1732:1736	arg1	thickening					1804:1813	smooth muscle thickening	1790:1813	smooth muscle thickening	1790:1813	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	10	24	theme	overt	1732:1736	arg1	deposition					1828:1837	collagen deposition	1819:1837	collagen deposition	1819:1837	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	4	25	theme	Electron	766:773	arg1	SEM					787:789	SEM	787:789	SEM	787:789	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	25	theme	Electron	766:773	arg1	Microscope					775:784	Scanning Electron Microscope	757:784	Scanning Electron Microscope (SEM)	757:790	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	25	theme	Electron	766:773	arg1	MATERIALS					735:743	MATERIALS	735:743	MATERIALS	735:743	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	17	26	theme	molecular	2869:2877	arg1	intermediates					2879:2891	various cellular, biochemical and molecular intermediates	2835:2891	various cellular, biochemical and molecular intermediates	2835:2891	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	16	27	theme	induced	2643:2649	arg1	secretion					2651:2659	the LPS induced secretion	2635:2659	the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells	2635:2700	In addition, under in-vitro conditions, MS-treatment attenuated the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells.
35202713	16	28	theme	-6	2671:2672	arg1	secretion					2651:2659	the LPS induced secretion	2635:2659	the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells	2635:2700	In addition, under in-vitro conditions, MS-treatment attenuated the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells.
35202713	3	29	theme	monocytic	635:643	arg1	THP-1 cells					645:655	LPS stimulated human monocytic THP-1 cells	614:655	LPS stimulated human monocytic THP-1 cells	614:655	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	15	30	theme	mice	2372:2375	arg1	MS-treatment					2341:2352	MS-treatment	2341:2352	MS-treatment	2341:2352	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	5	31	theme	Allergic	899:906	arg1	asthma					908:913	Allergic asthma	899:913	Allergic asthma	899:913	Allergic asthma was induced in Balb/c mice through intraperitoneal ovalbumin (OVA) injection.
35202713	6	32	theme	normal	1022:1027	arg1	control					1029:1035	normal control	1022:1035	normal control	1022:1035	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	0	33	theme	murine	78:83	arg1	model					85:89	murine model	78:89	murine model of allergic asthma	78:108	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	15	34	theme	collagen	2463:2470	arg1	deposition					2472:2481	peribronchial collagen deposition	2449:2481	peribronchial collagen deposition	2449:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	1	35	theme	ETHNOPHARMACOLOGICAL	163:182	arg1	disease					229:235	the leading inflammatory disease	204:235	the leading inflammatory disease of the airways with inadequate therapeutic options	204:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	1	35	theme	ETHNOPHARMACOLOGICAL	163:182	arg1	Asthma					194:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	4	36	theme	Electron	793:800	arg1	MATERIALS					735:743	MATERIALS	735:743	MATERIALS	735:743	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	36	theme	Electron	793:800	arg1	EDX					820:822	EDX	820:822	EDX	820:822	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	36	theme	Electron	793:800	arg1	X-ray					813:817	Electron Dispersive X-ray	793:817	Electron Dispersive X-ray (EDX)	793:823	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	14	37	theme	mRNA	2236:2239	arg1	levels					2252:2257	stimulated mRNA expression levels	2225:2257	stimulated mRNA expression levels	2225:2257	At the molecular level, MS-treatment reduced stimulated mRNA expression levels for IL-4, -5, -10, -13, -33, and IFN-γ cytokines.
35202713	14	37	theme	mRNA	2236:2239	arg1	cytokines					2298:2306	-5, -10, -13, -33, and IFN-γ cytokines	2269:2306	-5, -10, -13, -33, and IFN-γ cytokines	2269:2306	At the molecular level, MS-treatment reduced stimulated mRNA expression levels for IL-4, -5, -10, -13, -33, and IFN-γ cytokines.
35202713	15	38	theme	Histological	2309:2320	arg1	analysis					2322:2329	Histological analysis	2309:2329	Histological analysis following MS-treatment of OVA-stimulated mice lungs	2309:2381	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	10	39	theme	mice	1696:1699	arg1	OVA-challenge					1668:1680	OVA-challenge	1668:1680	OVA-challenge of the Balb/c mice	1668:1699	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	9	40	theme	arsenic	1535:1541	arg1	presence					1509:1516	the presence	1505:1516	the presence of mercury (Hg), arsenic (As), and sulphur (S)	1505:1563	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	9	41	from	elements	1582:1589	arg1	forms					1598:1602	the forms	1594:1602	the forms of mercury sulfide, arsenic trioxide, and their alloy crystals	1594:1665	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	6	42	theme	Experimental	993:1004	arg1	groups					1006:1011	Experimental groups	993:1011	Experimental groups	993:1011	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	8	43	theme	SEM	1360:1362	arg1	analysis					1370:1377	SEM image analysis	1360:1377	SEM image analysis	1360:1377	RESULTS SEM image analysis showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm.
35202713	2	44	theme	medicinal	406:414	arg1	system					416:421	the ancient traditional medicinal system	382:421	the ancient traditional medicinal system for treating chronic inflammations	382:456	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	0	45	theme	asthma	103:108	arg1	model					85:89	murine model	78:89	murine model of allergic asthma	78:108	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	7	46	theme	MS	1339:1340	arg1	efficacy					1342:1349	the MS efficacy	1335:1349	the MS efficacy	1335:1349	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	17	47	dep	CONCLUSION	2703:2712	arg1	suggest					2740:2746	suggest	2740:2746	suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates	2740:2891	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	15	48	from	thickening	2508:2517	arg1	deposition					2472:2481	peribronchial collagen deposition	2449:2481	peribronchial collagen deposition	2449:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	2	49	theme	ancient	386:392	arg1	system					416:421	the ancient traditional medicinal system	382:421	the ancient traditional medicinal system for treating chronic inflammations	382:456	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	0	50	theme	Herbo-metallic	0:13	arg1	Sindoor					36:42	Herbo-metallic ethnomedicine 'Malla Sindoor'	0:43	Herbo-metallic ethnomedicine 'Malla Sindoor'	0:43	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	5	51	theme	intraperitoneal	950:964	arg1	injection					982:990	intraperitoneal ovalbumin (OVA) injection	950:990	intraperitoneal ovalbumin (OVA) injection	950:990	Allergic asthma was induced in Balb/c mice through intraperitoneal ovalbumin (OVA) injection.
35202713	14	52	theme	molecular	2187:2195	arg1	level					2197:2201	the molecular level	2183:2201	the molecular level	2183:2201	At the molecular level, MS-treatment reduced stimulated mRNA expression levels for IL-4, -5, -10, -13, -33, and IFN-γ cytokines.
35202713	2	53	theme	chronic	436:442	arg1	inflammations					444:456	chronic inflammations	436:456	chronic inflammations	436:456	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	10	54	theme	Balb/c	1689:1694	arg1	mice					1696:1699	the Balb/c mice	1685:1699	the Balb/c mice	1685:1699	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	9	55	theme	sulphur	1553:1559	arg1	presence					1509:1516	the presence	1505:1516	the presence of mercury (Hg), arsenic (As), and sulphur (S)	1505:1563	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	3	56	theme	relevant	675:682	arg1	intermediates					720:732	the relevant cellular, biochemical and molecular intermediates	671:732	the relevant cellular, biochemical and molecular intermediates	671:732	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	4	57	theme	X-Ray	830:834	arg1	XRD					849:851	XRD	849:851	XRD	849:851	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	57	theme	X-Ray	830:834	arg1	MATERIALS					735:743	MATERIALS	735:743	MATERIALS	735:743	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	57	theme	X-Ray	830:834	arg1	Diffraction					836:846	X-Ray Diffraction	830:846	X-Ray Diffraction (XRD)	830:852	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	11	58	theme	asthma	1892:1897	arg1	features					1899:1906	allergic asthma features	1883:1906	allergic asthma features	1883:1906	Mice receiving MS-exhibited alleviation of allergic asthma features.
35202713	7	59	theme	Quantitative	1143:1154	arg1	PCR					1156:1158	Quantitative PCR	1143:1158	Quantitative PCR	1143:1158	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	13	60	theme	antioxidant	2124:2134	arg1	levels					2136:2141	antioxidant levels	2124:2141	antioxidant levels	2124:2141	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	7	61	theme	human	1291:1295	arg1	THP-1 cells					1307:1317	human monocytic THP-1 cells	1291:1317	human monocytic THP-1 cells for determining the MS efficacy	1291:1349	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	10	62	theme	muscle	1797:1802	arg1	thickening					1804:1813	smooth muscle thickening	1790:1813	smooth muscle thickening	1790:1813	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	17	63	theme	anti-asthmatic	2777:2790	arg1	MS					2753:2754	MS	2753:2754	MS	2753:2754	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	17	63	theme	anti-asthmatic	2777:2790	arg1	agent					2814:2818	an effective anti-asthmatic and anti-inflammatory agent	2764:2818	an effective anti-asthmatic and anti-inflammatory agent	2764:2818	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	10	64	theme	allergic	1764:1771	arg1	asthma					1773:1778	allergic asthma	1764:1778	allergic asthma	1764:1778	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	2	65	theme	ethnomedicinal	327:340	arg1	formulation					342:352	a metal-based ethnomedicinal formulation	313:352	a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations	313:456	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	2	65	theme	ethnomedicinal	327:340	arg1	Sindoor					296:302	'Malla Sindoor'	289:303	'Malla Sindoor' (MS)	289:308	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	3	66	theme	metallic	562:569	arg1	MS					580:581	traditional metallic medicine MS	550:581	traditional metallic medicine MS	550:581	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	9	67	theme	other	1576:1580	arg1	elements					1582:1589	other elements	1576:1589	other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals	1576:1665	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	11	68	theme	features	1899:1906	arg1	alleviation					1868:1878	MS-exhibited alleviation	1855:1878	MS-exhibited alleviation of allergic asthma features	1855:1906	Mice receiving MS-exhibited alleviation of allergic asthma features.
35202713	13	69	theme	cytokines	2110:2118	arg1	levels					2065:2070	the stimulated levels	2050:2070	the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines	2050:2118	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	13	69	theme	cytokines	2110:2118	arg1	levels					2136:2141	antioxidant levels	2124:2141	antioxidant levels	2124:2141	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	15	70	theme	muscle	2501:2506	arg1	thickening					2508:2517	bronchial smooth muscle thickening	2484:2517	bronchial smooth muscle thickening	2484:2517	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	1	71	theme	therapeutic	268:278	arg1	options					280:286	inadequate therapeutic options	257:286	inadequate therapeutic options	257:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	6	72	theme	MS	1089:1090	arg1	groups					1100:1105	three MS treated groups	1083:1105	three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg	1083:1140	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	72	theme	MS	1089:1090	arg1	13 mg/kg					1119:1126	13 mg/kg	1119:1126	13 mg/kg	1119:1126	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	72	theme	MS	1089:1090	arg1	39 mg/kg					1133:1140	39 mg/kg	1133:1140	39 mg/kg	1133:1140	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	72	theme	MS	1089:1090	arg1	4.3 mg/kg					1108:1116	4.3 mg/kg	1108:1116	4.3 mg/kg	1108:1116	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	17	73	theme	various	2835:2841	arg1	intermediates					2879:2891	various cellular, biochemical and molecular intermediates	2835:2891	various cellular, biochemical and molecular intermediates	2835:2891	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	3	74	theme	medicine	571:578	arg1	MS					580:581	traditional metallic medicine MS	550:581	traditional metallic medicine MS	550:581	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	15	75	theme	bronchial	2484:2492	arg1	thickening					2508:2517	bronchial smooth muscle thickening	2484:2517	bronchial smooth muscle thickening	2484:2517	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	13	76	theme	-6	2096:2097	arg1	levels					2065:2070	the stimulated levels	2050:2070	the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines	2050:2118	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	13	76	theme	-6	2096:2097	arg1	levels					2136:2141	antioxidant levels	2124:2141	antioxidant levels	2124:2141	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	12	77	theme	cell	1959:1962	arg1	count					1964:1968	the total cell count	1949:1968	the total cell count	1949:1968	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	78	from	decreases	1974:1982	arg1	neutrophils					1987:1997	neutrophils	1987:1997	neutrophils	1987:1997	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	78	from	decreases	1974:1982	arg1	count					1964:1968	the total cell count	1949:1968	the total cell count	1949:1968	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	78	from	decreases	1974:1982	arg1	lymphocytes					2011:2021	lymphocytes	2011:2021	lymphocytes	2011:2021	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	78	from	decreases	1974:1982	arg1	eosinophils					2028:2038	eosinophils	2028:2038	eosinophils	2028:2038	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	78	from	decreases	1974:1982	arg1	monocytes					2000:2008	monocytes	2000:2008	monocytes	2000:2008	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	9	79	theme	mercury	1607:1613	arg1	sulfide					1615:1621	mercury sulfide	1607:1621	mercury sulfide	1607:1621	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	3	80	theme	traditional	550:560	arg1	MS					580:581	traditional metallic medicine MS	550:581	traditional metallic medicine MS	550:581	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	16	81	from	THP-1 cells	2690:2700	arg1	secretion					2651:2659	the LPS induced secretion	2635:2659	the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells	2635:2700	In addition, under in-vitro conditions, MS-treatment attenuated the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells.
35202713	1	82	theme	RELEVANCE	184:192	arg1	disease					229:235	the leading inflammatory disease	204:235	the leading inflammatory disease of the airways with inadequate therapeutic options	204:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	1	82	theme	RELEVANCE	184:192	arg1	Asthma					194:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	7	83	theme	histological	1214:1225	arg1	analysis					1227:1234	histological analysis	1214:1234	histological analysis	1214:1234	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	3	84	theme	mice	596:599	arg1	model					601:605	asthmatic mice model	586:605	asthmatic mice model	586:605	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	9	85	theme	trioxide	1632:1639	arg1	forms					1598:1602	the forms	1594:1602	the forms of mercury sulfide, arsenic trioxide, and their alloy crystals	1594:1665	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	17	86	theme	biochemical	2853:2863	arg1	intermediates					2879:2891	various cellular, biochemical and molecular intermediates	2835:2891	various cellular, biochemical and molecular intermediates	2835:2891	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	15	87	theme	infiltration	2557:2568	arg1	decreases					2436:2444	decreases	2436:2444	decreases in peribronchial collagen deposition	2436:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	15	87	theme	infiltration	2557:2568	arg1	airways					2427:2433	airways	2427:2433	airways	2427:2433	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	15	87	theme	infiltration	2557:2568	arg1	thickening					2508:2517	bronchial smooth muscle thickening	2484:2517	bronchial smooth muscle thickening	2484:2517	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	15	87	theme	infiltration	2557:2568	arg1	attenuation					2524:2534	attenuation	2524:2534	attenuation of inflammatory cell infiltration	2524:2568	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	1	88	theme	leading	208:214	arg1	disease					229:235	the leading inflammatory disease	204:235	the leading inflammatory disease of the airways with inadequate therapeutic options	204:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	1	88	theme	leading	208:214	arg1	Asthma					194:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	8	89	theme	size	1437:1440	arg1	distribution					1442:1453	a particle size distribution	1426:1453	a particle size distribution between 100 nm-1 μm	1426:1473	RESULTS SEM image analysis showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm.
35202713	9	90	dep	arsenic	1535:1541	arg1	As					1544:1545	As	1544:1545	As	1544:1545	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	3	91	theme	LPS	614:616	arg1	THP-1 cells					645:655	LPS stimulated human monocytic THP-1 cells	614:655	LPS stimulated human monocytic THP-1 cells	614:655	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	15	92	theme	inflammatory	2539:2550	arg1	infiltration					2557:2568	inflammatory cell infiltration	2539:2568	inflammatory cell infiltration	2539:2568	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	9	93	theme	Elemental	1476:1484	arg1	composition					1486:1496	Elemental composition	1476:1496	Elemental composition	1476:1496	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	12	94	theme	fluid	1913:1917	arg1	analysis					1919:1926	BAL fluid analysis	1909:1926	BAL fluid analysis	1909:1926	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	9	95	theme	alloy	1652:1656	arg1	crystals					1658:1665	their alloy crystals	1646:1665	their alloy crystals	1646:1665	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	16	96	theme	TNF-α	2679:2683	arg1	secretion					2651:2659	the LPS induced secretion	2635:2659	the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells	2635:2700	In addition, under in-vitro conditions, MS-treatment attenuated the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells.
35202713	10	97	theme	pathological	1738:1749	arg1	features					1751:1758	overt pathological features	1732:1758	overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition	1732:1837	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	10	97	theme	pathological	1738:1749	arg1	thickening					1804:1813	smooth muscle thickening	1790:1813	smooth muscle thickening	1790:1813	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	10	97	theme	pathological	1738:1749	arg1	deposition					1828:1837	collagen deposition	1819:1837	collagen deposition	1819:1837	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	15	98	from	airways	2427:2433	arg1	deposition					2472:2481	peribronchial collagen deposition	2449:2481	peribronchial collagen deposition	2449:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	13	99	theme	stimulated	2054:2063	arg1	levels					2065:2070	the stimulated levels	2050:2070	the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines	2050:2118	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	10	100	theme	smooth	1790:1795	arg1	thickening					1804:1813	smooth muscle thickening	1790:1813	smooth muscle thickening	1790:1813	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	5	101	theme	Balb/c	930:935	arg1	mice					937:940	Balb/c mice	930:940	Balb/c mice	930:940	Allergic asthma was induced in Balb/c mice through intraperitoneal ovalbumin (OVA) injection.
35202713	6	102	theme	disease	1038:1044	arg1	control					1046:1052	disease control	1038:1052	disease control	1038:1052	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	4	103	theme	Scanning	757:764	arg1	SEM					787:789	SEM	787:789	SEM	787:789	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	103	theme	Scanning	757:764	arg1	Microscope					775:784	Scanning Electron Microscope	757:784	Scanning Electron Microscope (SEM)	757:790	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	103	theme	Scanning	757:764	arg1	MATERIALS					735:743	MATERIALS	735:743	MATERIALS	735:743	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	104	theme	MS	885:886	arg1	particles					888:896	MS particles	885:896	MS particles	885:896	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	17	105	theme	anti-inflammatory	2796:2812	arg1	MS					2753:2754	MS	2753:2754	MS	2753:2754	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	17	105	theme	anti-inflammatory	2796:2812	arg1	agent					2814:2818	an effective anti-asthmatic and anti-inflammatory agent	2764:2818	an effective anti-asthmatic and anti-inflammatory agent	2764:2818	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	3	106	theme	human	629:633	arg1	THP-1 cells					645:655	LPS stimulated human monocytic THP-1 cells	614:655	LPS stimulated human monocytic THP-1 cells	614:655	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	9	107	theme	mercury	1521:1527	arg1	presence					1509:1516	the presence	1505:1516	the presence of mercury (Hg), arsenic (As), and sulphur (S)	1505:1563	Elemental composition showed the presence of mercury (Hg), arsenic (As), and sulphur (S) along with other elements in the forms of mercury sulfide, arsenic trioxide, and their alloy crystals.
35202713	15	108	theme	OVA-stimulated	2357:2370	arg1	mice					2372:2375	OVA-stimulated mice	2357:2375	OVA-stimulated mice	2357:2375	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	0	109	dep	cytokines	124:132	arg1	status					134:139	status	134:139	status	134:139	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	0	109	dep	cytokines	124:132	arg1	stress					155:160	oxidative stress	145:160	oxidative stress	145:160	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	0	109	dep	cytokines	124:132	arg1	cytokines					124:132	cytokines	124:132	cytokines status and oxidative stress	124:160	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	14	110	theme	expression	2241:2250	arg1	levels					2252:2257	stimulated mRNA expression levels	2225:2257	stimulated mRNA expression levels	2225:2257	At the molecular level, MS-treatment reduced stimulated mRNA expression levels for IL-4, -5, -10, -13, -33, and IFN-γ cytokines.
35202713	14	110	theme	expression	2241:2250	arg1	cytokines					2298:2306	-5, -10, -13, -33, and IFN-γ cytokines	2269:2306	-5, -10, -13, -33, and IFN-γ cytokines	2269:2306	At the molecular level, MS-treatment reduced stimulated mRNA expression levels for IL-4, -5, -10, -13, -33, and IFN-γ cytokines.
35202713	0	111	theme	allergic	94:101	arg1	asthma					103:108	allergic asthma	94:108	allergic asthma	94:108	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	15	112	theme	MS-treatment	2341:2352	arg1	lungs					2377:2381	MS-treatment of OVA-stimulated mice lungs	2341:2381	MS-treatment of OVA-stimulated mice lungs	2341:2381	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	12	113	theme	BAL	1909:1911	arg1	analysis					1919:1926	BAL fluid analysis	1909:1926	BAL fluid analysis	1909:1926	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	4	114	dep	MATERIALS	735:743	arg1	XRD					849:851	XRD	849:851	XRD	849:851	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	114	dep	MATERIALS	735:743	arg1	X-ray					813:817	Electron Dispersive X-ray	793:817	Electron Dispersive X-ray (EDX)	793:823	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	114	dep	MATERIALS	735:743	arg1	Microscope					775:784	Scanning Electron Microscope	757:784	Scanning Electron Microscope (SEM)	757:790	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	114	dep	MATERIALS	735:743	arg1	MATERIALS					735:743	MATERIALS	735:743	MATERIALS	735:743	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	114	dep	MATERIALS	735:743	arg1	Diffraction					836:846	X-Ray Diffraction	830:846	X-Ray Diffraction (XRD)	830:852	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	114	dep	MATERIALS	735:743	arg1	SEM					787:789	SEM	787:789	SEM	787:789	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	114	dep	MATERIALS	735:743	arg1	EDX					820:822	EDX	820:822	EDX	820:822	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	7	115	theme	anti-oxidant	1188:1199	arg1	enzymes					1201:1207	anti-oxidant enzymes	1188:1207	anti-oxidant enzymes	1188:1207	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	14	116	theme	stimulated	2225:2234	arg1	levels					2252:2257	stimulated mRNA expression levels	2225:2257	stimulated mRNA expression levels	2225:2257	At the molecular level, MS-treatment reduced stimulated mRNA expression levels for IL-4, -5, -10, -13, -33, and IFN-γ cytokines.
35202713	14	116	theme	stimulated	2225:2234	arg1	cytokines					2298:2306	-5, -10, -13, -33, and IFN-γ cytokines	2269:2306	-5, -10, -13, -33, and IFN-γ cytokines	2269:2306	At the molecular level, MS-treatment reduced stimulated mRNA expression levels for IL-4, -5, -10, -13, -33, and IFN-γ cytokines.
35202713	8	117	dep	RESULTS	1352:1358	arg1	showed					1379:1384	showed	1379:1384	showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm	1379:1473	RESULTS SEM image analysis showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm.
35202713	17	118	theme	effective	2767:2775	arg1	MS					2753:2754	MS	2753:2754	MS	2753:2754	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	17	118	theme	effective	2767:2775	arg1	agent					2814:2818	an effective anti-asthmatic and anti-inflammatory agent	2764:2818	an effective anti-asthmatic and anti-inflammatory agent	2764:2818	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	13	119	theme	TNF-α	2104:2108	arg1	cytokines					2110:2118	TNF-α cytokines	2104:2118	TNF-α cytokines	2104:2118	Further, the stimulated levels of interleukin (IL)-1β, -6, and TNF-α cytokines and antioxidant levels were also reduced upon MS-treatment.
35202713	4	120	theme	Dispersive	802:811	arg1	MATERIALS					735:743	MATERIALS	735:743	MATERIALS	735:743	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	120	theme	Dispersive	802:811	arg1	EDX					820:822	EDX	820:822	EDX	820:822	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	4	120	theme	Dispersive	802:811	arg1	X-ray					813:817	Electron Dispersive X-ray	793:817	Electron Dispersive X-ray (EDX)	793:823	MATERIALS AND METHODS Scanning Electron Microscope (SEM), Electron Dispersive X-ray (EDX), and X-Ray Diffraction (XRD) were performed to characterize MS particles.
35202713	2	121	theme	traditional	394:404	arg1	system					416:421	the ancient traditional medicinal system	382:421	the ancient traditional medicinal system for treating chronic inflammations	382:456	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	0	122	theme	ethnomedicine	15:27	arg1	Sindoor					36:42	Herbo-metallic ethnomedicine 'Malla Sindoor'	0:43	Herbo-metallic ethnomedicine 'Malla Sindoor'	0:43	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	16	123	theme	IL-1β	2664:2668	arg1	secretion					2651:2659	the LPS induced secretion	2635:2659	the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells	2635:2700	In addition, under in-vitro conditions, MS-treatment attenuated the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells.
35202713	8	124	theme	image	1364:1368	arg1	analysis					1370:1377	SEM image analysis	1360:1377	SEM image analysis	1360:1377	RESULTS SEM image analysis showed the MS to be heterogenous in shape with a particle size distribution between 100 nm-1 μm.
35202713	0	125	theme	Malla	30:34	arg1	Sindoor					36:42	Herbo-metallic ethnomedicine 'Malla Sindoor'	0:43	Herbo-metallic ethnomedicine 'Malla Sindoor'	0:43	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	1	126	with	disease	229:235	arg1	options					280:286	inadequate therapeutic options	257:286	inadequate therapeutic options	257:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	15	127	theme	peribronchial	2449:2461	arg1	deposition					2472:2481	peribronchial collagen deposition	2449:2481	peribronchial collagen deposition	2449:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	5	128	theme	ovalbumin	966:974	arg1	injection					982:990	intraperitoneal ovalbumin (OVA) injection	950:990	intraperitoneal ovalbumin (OVA) injection	950:990	Allergic asthma was induced in Balb/c mice through intraperitoneal ovalbumin (OVA) injection.
35202713	7	129	theme	monocytic	1297:1305	arg1	THP-1 cells					1307:1317	human monocytic THP-1 cells	1291:1317	human monocytic THP-1 cells for determining the MS efficacy	1291:1349	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	10	130	theme	collagen	1819:1826	arg1	deposition					1828:1837	collagen deposition	1819:1837	collagen deposition	1819:1837	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	15	131	from	attenuation	2524:2534	arg1	deposition					2472:2481	peribronchial collagen deposition	2449:2481	peribronchial collagen deposition	2449:2481	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	17	132	theme	cellular	2843:2850	arg1	intermediates					2879:2891	various cellular, biochemical and molecular intermediates	2835:2891	various cellular, biochemical and molecular intermediates	2835:2891	CONCLUSION Collectively, the results suggest that MS acts as an effective anti-asthmatic and anti-inflammatory agent, by regulating various cellular, biochemical and molecular intermediates.
35202713	3	133	theme	STUDY	470:474	arg1	AIM					459:461	AIM	459:461	AIM OF THE STUDY	459:474	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	0	134	theme	lung	57:60	arg1	inflammation					62:73	lung inflammation	57:73	lung inflammation in murine model of allergic asthma	57:108	Herbo-metallic ethnomedicine 'Malla Sindoor' ameliorates lung inflammation in murine model of allergic asthma by modulating cytokines status and oxidative stress.
35202713	12	135	from	decrease	1937:1944	arg1	neutrophils					1987:1997	neutrophils	1987:1997	neutrophils	1987:1997	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	135	from	decrease	1937:1944	arg1	count					1964:1968	the total cell count	1949:1968	the total cell count	1949:1968	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	135	from	decrease	1937:1944	arg1	lymphocytes					2011:2021	lymphocytes	2011:2021	lymphocytes	2011:2021	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	135	from	decrease	1937:1944	arg1	eosinophils					2028:2038	eosinophils	2028:2038	eosinophils	2028:2038	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	12	135	from	decrease	1937:1944	arg1	monocytes					2000:2008	monocytes	2000:2008	monocytes	2000:2008	BAL fluid analysis showed a decrease in the total cell count and decreases in neutrophils, monocytes, lymphocytes, and eosinophils.
35202713	16	136	theme	in-vitro	2590:2597	arg1	conditions					2599:2608	in-vitro conditions	2590:2608	in-vitro conditions	2590:2608	In addition, under in-vitro conditions, MS-treatment attenuated the LPS induced secretion of IL-1β, -6, and TNF-α from THP-1 cells.
35202713	3	137	theme	biochemical	694:704	arg1	intermediates					720:732	the relevant cellular, biochemical and molecular intermediates	671:732	the relevant cellular, biochemical and molecular intermediates	671:732	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	6	138	dep	groups	1100:1105	arg1	groups					1100:1105	three MS treated groups	1083:1105	three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg	1083:1140	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	138	dep	groups	1100:1105	arg1	13 mg/kg					1119:1126	13 mg/kg	1119:1126	13 mg/kg	1119:1126	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	138	dep	groups	1100:1105	arg1	39 mg/kg					1133:1140	39 mg/kg	1133:1140	39 mg/kg	1133:1140	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	6	138	dep	groups	1100:1105	arg1	4.3 mg/kg					1108:1116	4.3 mg/kg	1108:1116	4.3 mg/kg	1108:1116	Experimental groups include, normal control, disease control, Dexamethasone (2 mg/kg) and three MS treated groups: 4.3 mg/kg, 13 mg/kg, and 39 mg/kg.
35202713	3	139	theme	anti-inflammatory	499:515	arg1	properties					536:545	the anti-inflammatory and anti-asthmatic properties	495:545	the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS	495:581	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	10	140	theme	features	1751:1758	arg1	development					1717:1727	the development	1713:1727	the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition	1713:1837	OVA-challenge of the Balb/c mice resulted in the development of overt pathological features for allergic asthma including smooth muscle thickening and collagen deposition.
35202713	7	141	theme	treated	1259:1265	arg1	mice					1267:1270	the treated mice	1255:1270	the treated mice	1255:1270	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	3	142	theme	cellular	684:691	arg1	intermediates					720:732	the relevant cellular, biochemical and molecular intermediates	671:732	the relevant cellular, biochemical and molecular intermediates	671:732	AIM OF THE STUDY Here, we validated the anti-inflammatory and anti-asthmatic properties of traditional metallic medicine MS in asthmatic mice model and in LPS stimulated human monocytic THP-1 cells, by examining the relevant cellular, biochemical and molecular intermediates.
35202713	2	143	theme	metal-based	315:325	arg1	formulation					342:352	a metal-based ethnomedicinal formulation	313:352	a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations	313:456	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	2	143	theme	metal-based	315:325	arg1	Sindoor					296:302	'Malla Sindoor'	289:303	'Malla Sindoor' (MS)	289:308	'Malla Sindoor' (MS) is a metal-based ethnomedicinal formulation that has been prescribed in the ancient traditional medicinal system for treating chronic inflammations.
35202713	1	144	theme	airways	244:250	arg1	disease					229:235	the leading inflammatory disease	204:235	the leading inflammatory disease of the airways with inadequate therapeutic options	204:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	1	144	theme	airways	244:250	arg1	Asthma					194:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma	163:199	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
35202713	11	145	theme	allergic	1883:1890	arg1	features					1899:1906	allergic asthma features	1883:1906	allergic asthma features	1883:1906	Mice receiving MS-exhibited alleviation of allergic asthma features.
35202713	7	146	theme	inflammatory	1161:1172	arg1	cytokines					1174:1182	inflammatory cytokines	1161:1182	inflammatory cytokines	1161:1182	Quantitative PCR, inflammatory cytokines and anti-oxidant enzymes, and histological analysis were performed, in the treated mice and LPS stimulated human monocytic THP-1 cells for determining the MS efficacy.
35202713	15	147	theme	smooth	2494:2499	arg1	thickening					2508:2517	bronchial smooth muscle thickening	2484:2517	bronchial smooth muscle thickening	2484:2517	Histological analysis following MS-treatment of OVA-stimulated mice lungs showed a reduction in mucus accumulation in airways, decreases in peribronchial collagen deposition, bronchial smooth muscle thickening, and attenuation of inflammatory cell infiltration.
35202713	1	148	theme	inadequate	257:266	arg1	options					280:286	inadequate therapeutic options	257:286	inadequate therapeutic options	257:286	ETHNOPHARMACOLOGICAL RELEVANCE Asthma is the leading inflammatory disease of the airways with inadequate therapeutic options.
37400005	12	0	from	reduction	1782:1790	arg1	levels					1811:1816	TNF-α and IL-1β levels	1795:1816	TNF-α and IL-1β levels	1795:1816	In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed.
37400005	12	0	from	reduction	1782:1790	arg1	migration					1832:1840	leukocyte migration	1822:1840	leukocyte migration	1822:1840	In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed.
37400005	15	1	dep	CONCLUSION	2006:2015	arg1	support					2047:2053	support	2047:2053	not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications	2038:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	1	dep	CONCLUSION	2006:2015	arg1	demonstrate					2152:2162	demonstrate	2152:2162	not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications	2038:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	14	2	from	standard	1981:1988	arg1	models					1998:2003	both models	1993:2003	both models	1993:2003	The reduction in paw edema was statistically superior to the standard in both models.
37400005	9	3	theme	acute	1434:1438	arg1	toxicity					1440:1447	No acute toxicity	1431:1447	No acute toxicity	1431:1447	RESULTS No acute toxicity was observed at doses up to 2000 mg/kg; p.o.
37400005	7	4	theme	antinociceptive	1049:1063	arg1	effect					1065:1070	The antinociceptive effect	1045:1070	The antinociceptive effect	1045:1070	The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception.
37400005	14	5	from	reduction	1924:1932	arg1	edema					1941:1945	paw edema	1937:1945	paw edema	1937:1945	The reduction in paw edema was statistically superior to the standard in both models.
37400005	1	6	theme	Brazil	166:171	arg1	region					143:148	the semi-arid region	129:148	the semi-arid region of Northeastern Brazil	129:171	from the semi-arid region of Northeastern Brazil.
37400005	5	7	theme	acute	830:834	arg1	toxicity					841:848	acute oral toxicity	830:848	acute oral toxicity	830:848	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	8	8	theme	anti-inflammatory	1250:1266	arg1	effect					1268:1273	The acute anti-inflammatory effect	1240:1273	The acute anti-inflammatory effect	1240:1273	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	2	9	theme	medicinal	250:258	arg1	RELEVANCE					195:203	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith	174:243	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith	174:243	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	2	9	theme	medicinal	250:258	arg1	plant					260:264	a medicinal plant	248:264	a medicinal plant	248:264	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	15	10	theme	phytocomponents	2194:2208	arg1	source					2184:2189	a rich source	2177:2189	a rich source	2177:2189	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	10	theme	phytocomponents	2194:2208	arg1	agent					2289:2293	a natural and sustainable therapeutic agent	2251:2293	a natural and sustainable therapeutic agent with industrial applications	2251:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	10	theme	phytocomponents	2194:2208	arg1	this					2169:2172	this	2169:2172	this	2169:2172	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	9	11	dep	RESULTS	1423:1429	arg1	observed					1453:1460	observed	1453:1460	was observed at doses up to 2000 mg/kg	1449:1486	RESULTS No acute toxicity was observed at doses up to 2000 mg/kg; p.o.
37400005	7	12	theme	possible	1181:1188	arg1	mechanisms					1190:1199	the possible mechanisms	1177:1199	the possible mechanisms of action involved in antinociception	1177:1237	The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception.
37400005	0	13	theme	anti-inflammatory	20:36	arg1	activities					38:47	Antinociceptive and anti-inflammatory activities	0:47	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão)	0:110	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	1	14	theme	semi-arid	133:141	arg1	region					143:148	the semi-arid region	129:148	the semi-arid region of Northeastern Brazil	129:171	from the semi-arid region of Northeastern Brazil.
37400005	15	15	theme	sustainable	2265:2275	arg1	source					2184:2189	a rich source	2177:2189	a rich source	2177:2189	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	15	theme	sustainable	2265:2275	arg1	agent					2289:2293	a natural and sustainable therapeutic agent	2251:2293	a natural and sustainable therapeutic agent with industrial applications	2251:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	4	16	theme	properties	651:660	arg1	none					618:621	none	618:621	none of the ethnopharmacological properties	618:660	However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil).
37400005	15	17	theme	rich	2179:2182	arg1	source					2184:2189	a rich source	2177:2189	a rich source	2177:2189	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	17	theme	rich	2179:2182	arg1	agent					2289:2293	a natural and sustainable therapeutic agent	2251:2293	a natural and sustainable therapeutic agent with industrial applications	2251:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	17	theme	rich	2179:2182	arg1	this					2169:2172	this	2169:2172	this	2169:2172	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	2	18	theme	ETHNOPHARMACOLOGICAL	174:193	arg1	RELEVANCE					195:203	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith	174:243	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith	174:243	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	2	18	theme	ETHNOPHARMACOLOGICAL	174:193	arg1	plant					260:264	a medicinal plant	248:264	a medicinal plant	248:264	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	8	19	theme	peritonitis	1330:1340	arg1	models					1300:1305	models	1300:1305	models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation	1300:1420	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	0	20	dep	the	69:71	arg1	leaves					73:78	leaves	73:78	leaves	73:78	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	8	21	theme	yeast-induced	1343:1355	arg1	pyrexia					1357:1363	yeast-induced pyrexia	1343:1363	yeast-induced pyrexia	1343:1363	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	2	22	theme	cheiro	361:366	arg1	"					367:367	"amburana de cheiro"	348:367	"amburana de cheiro"	348:367	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	7	23	theme	acetic	1145:1150	arg1	acid					1152:1155	acetic acid	1145:1155	acetic acid	1145:1155	The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception.
37400005	15	24	theme	traditional	2059:2069	arg1	use					2071:2073	the traditional use	2055:2073	the traditional use of the species in inflammatory conditions and pain in folk medicine	2055:2141	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	4	25	theme	volatile	702:709	arg1	compounds					711:719	volatile compounds	702:719	volatile compounds obtained from its leaves (essential oil)	702:760	However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil).
37400005	2	26	theme	amburana	349:356	arg1	"					367:367	"amburana de cheiro"	348:367	"amburana de cheiro"	348:367	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	0	27	theme	Antinociceptive	0:14	arg1	activities					38:47	Antinociceptive and anti-inflammatory activities	0:47	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão)	0:110	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	14	28	from	models	1998:2003	arg1	standard					1981:1988	standard	1981:1988	standard	1981:1988	The reduction in paw edema was statistically superior to the standard in both models.
37400005	6	29	theme	oil	1015:1017	arg1	METHODS					971:977	METHODS	971:977	METHODS	971:977	MATERIAL AND METHODS The acute toxicity of the essential oil was investigated in mice.
37400005	6	29	theme	oil	1015:1017	arg1	MATERIAL					958:965	MATERIAL	958:965	MATERIAL	958:965	MATERIAL AND METHODS The acute toxicity of the essential oil was investigated in mice.
37400005	3	30	contain	have	499:502	arg2	use					520:522	their practical use	504:522	their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain	504:606	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	3	30	contain	have	499:502	arg1	infusions					436:444	infusions	436:444	infusions	436:444	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	3	30	contain	have	499:502	arg1	decoctions					456:465	decoctions	456:465	decoctions	456:465	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	3	30	contain	have	499:502	arg1	teas					447:450	teas	447:450	teas	447:450	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	10	31	theme	antinociceptive	1498:1512	arg1	effect					1514:1519	The antinociceptive effect	1494:1519	The antinociceptive effect	1494:1519	The antinociceptive effect was statistically equal to morphine.
37400005	10	31	theme	antinociceptive	1498:1512	arg1	equal					1539:1543	equal	1539:1543	equal	1539:1543	The antinociceptive effect was statistically equal to morphine.
37400005	8	32	theme	carrageenan-	1370:1381	arg1	inflammation					1409:1420	carrageenan- and histamine-induced paw inflammation	1370:1420	carrageenan- and histamine-induced paw inflammation	1370:1420	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	7	33	theme	abdominal	1115:1123	arg1	writhing					1125:1132	abdominal writhing	1115:1132	abdominal writhing	1115:1132	The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception.
37400005	3	34	theme	leaves	470:475	arg1	infusions					436:444	infusions	436:444	infusions	436:444	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	3	34	theme	leaves	470:475	arg1	decoctions					456:465	decoctions	456:465	decoctions	456:465	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	3	34	theme	leaves	470:475	arg1	teas					447:450	teas	447:450	teas	447:450	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	5	35	theme	oil	921:923	arg1	composition					817:827	the chemical composition	804:827	the chemical composition	804:827	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	5	35	theme	oil	921:923	arg1	toxicity					841:848	acute oral toxicity	830:848	acute oral toxicity	830:848	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	5	35	theme	oil	921:923	arg1	activities					893:902	antinociceptive and anti-inflammatory activities	855:902	antinociceptive and anti-inflammatory activities	855:902	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	15	36	used	used	2243:2246	arg2	this					2169:2172	this	2169:2172	this	2169:2172	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	36	used	used	2243:2246	arg2	agent					2289:2293	a natural and sustainable therapeutic agent	2251:2293	a natural and sustainable therapeutic agent with industrial applications	2251:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	36	used	used	2243:2246	arg2	source					2184:2189	a rich source	2177:2189	a rich source	2177:2189	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	8	37	theme	histamine-induced	1387:1403	arg1	inflammation					1409:1420	carrageenan- and histamine-induced paw inflammation	1370:1420	carrageenan- and histamine-induced paw inflammation	1370:1420	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	3	38	theme	semi-arid	395:403	arg1	region					405:410	the semi-arid region	391:410	the semi-arid region of Northeastern Brazil	391:433	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	12	39	theme	IL-1β	1805:1809	arg1	levels					1811:1816	TNF-α and IL-1β levels	1795:1816	TNF-α and IL-1β levels	1795:1816	In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed.
37400005	0	40	theme	the	69:71	arg1	oil					62:64	essential oil	52:64	essential oil of the leaves of Amburana cearensis (Allemão)	52:110	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	8	41	theme	inflammation	1409:1420	arg1	models					1300:1305	models	1300:1305	models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation	1300:1420	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	11	42	theme	analgesic	1596:1604	arg1	activity					1606:1613	analgesic activity	1596:1613	analgesic activity	1596:1613	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	7	43	theme	formalin	1096:1103	arg1	test					1105:1108	the formalin test	1092:1108	the formalin test	1092:1108	The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception.
37400005	11	44	dep	mechanisms	1668:1677	arg1	system					1710:1715	the cholinergic, adenosinergic system	1679:1715	system	1710:1715	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	12	45	theme	leukocyte	1822:1830	arg1	migration					1832:1840	leukocyte migration	1822:1840	leukocyte migration	1822:1840	In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed.
37400005	11	46	theme	potassium	1736:1744	arg1	K-ATP					1756:1760	K-ATP	1756:1760	K-ATP	1756:1760	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	11	46	theme	potassium	1736:1744	arg1	channels					1746:1753	ATP-sensitive potassium channels	1722:1753	ATP-sensitive potassium channels (K-ATP)	1722:1761	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	5	47	dep	investigated	791:802	arg1	AIM					763:765	AIM	763:765	AIM OF THE STUDY	763:778	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	15	48	with	agent	2289:2293	arg1	applications					2311:2322	industrial applications	2300:2322	industrial applications	2300:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	3	49	theme	Brazil	428:433	arg1	region					405:410	the semi-arid region	391:410	the semi-arid region of Northeastern Brazil	391:433	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	5	50	theme	STUDY	774:778	arg1	AIM					763:765	AIM	763:765	AIM OF THE STUDY	763:778	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	2	51	dep	RELEVANCE	195:203	arg1	Allemão					225:231	Allemão	225:231	Allemão	225:231	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	11	52	theme	neurogenic	1622:1631	arg1	phases					1650:1655	the neurogenic and inflammatory phases	1618:1655	the neurogenic and inflammatory phases	1618:1655	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	15	53	theme	species	2082:2088	arg1	use					2071:2073	the traditional use	2055:2073	the traditional use of the species in inflammatory conditions and pain in folk medicine	2055:2141	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	12	54	theme	TNF-α	1795:1799	arg1	levels					1811:1816	TNF-α and IL-1β levels	1795:1816	TNF-α and IL-1β levels	1795:1816	In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed.
37400005	2	55	theme	cumaru	337:342	arg1	"					343:343	"cumaru"	336:343	"cumaru"	336:343	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	15	56	theme	therapeutic	2277:2287	arg1	source					2184:2189	a rich source	2177:2189	a rich source	2177:2189	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	56	theme	therapeutic	2277:2287	arg1	agent					2289:2293	a natural and sustainable therapeutic agent	2251:2293	a natural and sustainable therapeutic agent with industrial applications	2251:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	57	from	use	2071:2073	arg1	pain					2121:2124	pain	2121:2124	pain in folk medicine	2121:2141	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	57	from	use	2071:2073	arg1	conditions					2106:2115	inflammatory conditions	2093:2115	inflammatory conditions	2093:2115	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	11	58	theme	inflammatory	1637:1648	arg1	phases					1650:1655	the neurogenic and inflammatory phases	1618:1655	the neurogenic and inflammatory phases	1618:1655	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	3	59	theme	Amburana	480:487	arg1	cearensis					489:497	Amburana cearensis	480:497	Amburana cearensis	480:497	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	5	60	from	the	930:932	arg1	composition					817:827	the chemical composition	804:827	the chemical composition	804:827	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	5	60	from	the	930:932	arg1	oil					921:923	the essential oil	907:923	the essential oil from the leaves of A. cearensis	907:955	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	5	60	from	the	930:932	arg1	toxicity					841:848	acute oral toxicity	830:848	acute oral toxicity	830:848	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	5	60	from	the	930:932	arg1	activities					893:902	antinociceptive and anti-inflammatory activities	855:902	antinociceptive and anti-inflammatory activities	855:902	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	9	61	located	observed	1453:1460	arg2	toxicity					1440:1447	No acute toxicity	1431:1447	No acute toxicity	1431:1447	RESULTS No acute toxicity was observed at doses up to 2000 mg/kg; p.o.
37400005	9	61	located	observed	1453:1460	arg2	p.o.					1489:1492	p.o.	1489:1492	p.o.	1489:1492	RESULTS No acute toxicity was observed at doses up to 2000 mg/kg; p.o.
37400005	9	61	located	observed	1453:1460	arg1	doses					1465:1469	doses	1465:1469	doses up to 2000 mg/kg	1465:1486	RESULTS No acute toxicity was observed at doses up to 2000 mg/kg; p.o.
37400005	4	62	dep	its	735:737	arg1	leaves					739:744	leaves	739:744	leaves	739:744	However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil).
37400005	2	63	theme	wide	271:274	arg1	distribution					276:287	wide distribution	271:287	wide distribution in South America	271:304	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	5	64	theme	chemical	808:815	arg1	composition					817:827	the chemical composition	804:827	the chemical composition	804:827	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	0	65	theme	oil	62:64	arg1	activities					38:47	Antinociceptive and anti-inflammatory activities	0:47	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão)	0:110	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	3	66	theme	practical	510:518	arg1	use					520:522	their practical use	504:522	their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain	504:606	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	11	67	theme	adenosinergic	1696:1708	arg1	system					1710:1715	the cholinergic, adenosinergic system	1679:1715	system	1710:1715	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	15	68	from	conditions	2106:2115	arg1	medicine					2134:2141	folk medicine	2129:2141	folk medicine	2129:2141	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	7	69	dep	evaluated	1076:1084	arg1	induced					1134:1140	induced	1134:1140	induced	1134:1140	The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception.
37400005	11	70	theme	formalin	1565:1572	arg1	assay					1574:1578	the formalin assay	1561:1578	the formalin assay	1561:1578	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	12	71	located	observed	1847:1854	arg1	peritonitis					1767:1777	peritonitis	1767:1777	peritonitis	1767:1777	In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed.
37400005	12	71	located	observed	1847:1854	arg2	reduction					1782:1790	a reduction	1780:1790	a reduction in TNF-α and IL-1β levels and leukocyte migration	1780:1840	In peritonitis, a reduction in TNF-α and IL-1β levels and leukocyte migration were observed.
37400005	15	72	from	pain	2121:2124	arg1	medicine					2134:2141	folk medicine	2129:2141	folk medicine	2129:2141	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	2	73	dep	Allemão	225:231	arg1	Smith					239:243	Smith	239:243	Smith	239:243	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	15	74	theme	folk	2129:2132	arg1	medicine					2134:2141	folk medicine	2129:2141	folk medicine	2129:2141	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	5	75	theme	antinociceptive	855:869	arg1	activities					893:902	antinociceptive and anti-inflammatory activities	855:902	antinociceptive and anti-inflammatory activities	855:902	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	2	76	with	plant	260:264	arg1	distribution					276:287	wide distribution	271:287	wide distribution in South America	271:304	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	8	77	theme	acute	1244:1248	arg1	effect					1268:1273	The acute anti-inflammatory effect	1240:1273	The acute anti-inflammatory effect	1240:1273	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	5	78	theme	anti-inflammatory	875:891	arg1	activities					893:902	antinociceptive and anti-inflammatory activities	855:902	antinociceptive and anti-inflammatory activities	855:902	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	6	79	theme	acute	983:987	arg1	toxicity					989:996	The acute toxicity	979:996	The acute toxicity	979:996	MATERIAL AND METHODS The acute toxicity of the essential oil was investigated in mice.
37400005	5	80	dep	the	930:932	arg1	leaves					934:939	leaves	934:939	leaves	934:939	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	3	81	theme	gastrointestinal	544:559	arg1	disorders					561:569	gastrointestinal disorders	544:569	gastrointestinal disorders	544:569	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	5	82	theme	oral	836:839	arg1	toxicity					841:848	acute oral toxicity	830:848	acute oral toxicity	830:848	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	2	83	from	distribution	276:287	arg1	America					298:304	America	298:304	America	298:304	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	0	84	theme	Amburana	83:90	arg1	Allemão					103:109	Allemão	103:109	Allemão	103:109	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	0	84	theme	Amburana	83:90	arg1	cearensis					92:100	Amburana cearensis	83:100	Amburana cearensis (Allemão)	83:110	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	5	85	from	composition	817:827	arg1	the					930:932	the	930:932	the	930:932	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	0	86	dep	Smith	117:121	arg1	activities					38:47	Antinociceptive and anti-inflammatory activities	0:47	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão)	0:110	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	6	87	dep	MATERIAL	958:965	arg1	toxicity					989:996	The acute toxicity	979:996	The acute toxicity	979:996	MATERIAL AND METHODS The acute toxicity of the essential oil was investigated in mice.
37400005	4	88	theme	ethnopharmacological	630:649	arg1	properties					651:660	the ethnopharmacological properties	626:660	the ethnopharmacological properties	626:660	However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil).
37400005	9	89	dep	2000 mg/kg	1477:1486	arg1	up					1471:1472	up	1471:1472	up	1471:1472	RESULTS No acute toxicity was observed at doses up to 2000 mg/kg; p.o.
37400005	8	90	theme	carrageenan-induced	1310:1328	arg1	peritonitis					1330:1340	carrageenan-induced peritonitis	1310:1340	carrageenan-induced peritonitis	1310:1340	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	5	91	from	toxicity	841:848	arg1	the					930:932	the	930:932	the	930:932	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	15	92	theme	inflammatory	2093:2104	arg1	conditions					2106:2115	inflammatory conditions	2093:2115	inflammatory conditions	2093:2115	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	13	93	theme	antipyretic	1861:1871	arg1	superior					1898:1905	superior	1898:1905	superior	1898:1905	The antipyretic effect was statistically superior to dipyrone.
37400005	13	93	theme	antipyretic	1861:1871	arg1	effect					1873:1878	The antipyretic effect	1857:1878	The antipyretic effect	1857:1878	The antipyretic effect was statistically superior to dipyrone.
37400005	2	94	theme	de	358:359	arg1	"					367:367	"amburana de cheiro"	348:367	"amburana de cheiro"	348:367	ETHNOPHARMACOLOGICAL RELEVANCE Amburana cearensis (Allemão) A.C. Smith is a medicinal plant with wide distribution in South America, popularly known in Brazil as "cumaru" or "amburana de cheiro".
37400005	5	95	from	activities	893:902	arg1	the					930:932	the	930:932	the	930:932	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	8	96	theme	pyrexia	1357:1363	arg1	models					1300:1305	models	1300:1305	models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation	1300:1420	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	15	97	theme	natural	2253:2259	arg1	source					2184:2189	a rich source	2177:2189	a rich source	2177:2189	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	15	97	theme	natural	2253:2259	arg1	agent					2289:2293	a natural and sustainable therapeutic agent	2251:2293	a natural and sustainable therapeutic agent with industrial applications	2251:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
37400005	5	98	theme	essential	911:919	arg1	oil					921:923	the essential oil	907:923	the essential oil from the leaves of A. cearensis	907:955	AIM OF THE STUDY This study investigated the chemical composition, acute oral toxicity, and antinociceptive and anti-inflammatory activities of the essential oil from the leaves of A. cearensis.
37400005	6	99	theme	essential	1005:1013	arg1	oil					1015:1017	the essential oil	1001:1017	the essential oil	1001:1017	MATERIAL AND METHODS The acute toxicity of the essential oil was investigated in mice.
37400005	3	100	theme	inflammation	590:601	arg1	pain					603:606	inflammation pain	590:606	inflammation pain	590:606	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	0	101	theme	essential	52:60	arg1	oil					62:64	essential oil	52:64	essential oil of the leaves of Amburana cearensis (Allemão)	52:110	Antinociceptive and anti-inflammatory activities of essential oil of the leaves of Amburana cearensis (Allemão) A.C. Smith.
37400005	3	102	theme	folk	373:376	arg1	medicine					378:385	folk medicine	373:385	folk medicine	373:385	In folk medicine, in the semi-arid region of Northeastern Brazil, infusions, teas and decoctions of leaves of Amburana cearensis have their practical use for treating fever, gastrointestinal disorders, inflammation, and inflammation pain.
37400005	11	103	theme	cholinergic	1683:1693	arg1	system					1710:1715	the cholinergic, adenosinergic system	1679:1715	system	1710:1715	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	11	104	theme	ATP-sensitive	1722:1734	arg1	K-ATP					1756:1760	K-ATP	1756:1760	K-ATP	1756:1760	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	11	104	theme	ATP-sensitive	1722:1734	arg1	channels					1746:1753	ATP-sensitive potassium channels	1722:1753	ATP-sensitive potassium channels (K-ATP)	1722:1761	In the formalin assay, the oil showed analgesic activity in the neurogenic and inflammatory phases, having as mechanisms the cholinergic, adenosinergic system, and ATP-sensitive potassium channels (K-ATP).
37400005	8	105	theme	paw	1405:1407	arg1	inflammation					1409:1420	carrageenan- and histamine-induced paw inflammation	1370:1420	carrageenan- and histamine-induced paw inflammation	1370:1420	The acute anti-inflammatory effect was investigated through models of carrageenan-induced peritonitis, yeast-induced pyrexia, and carrageenan- and histamine-induced paw inflammation.
37400005	4	106	theme	essential	747:755	arg1	its					735:737	its	735:737	its	735:737	However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil).
37400005	4	106	theme	essential	747:755	arg1	oil					757:759	essential oil	747:759	essential oil	747:759	However, none of the ethnopharmacological properties has been scientifically evaluated using volatile compounds obtained from its leaves (essential oil).
37400005	14	107	theme	paw	1937:1939	arg1	edema					1941:1945	paw edema	1937:1945	paw edema	1937:1945	The reduction in paw edema was statistically superior to the standard in both models.
37400005	7	108	theme	action	1204:1209	arg1	mechanisms					1190:1199	the possible mechanisms	1177:1199	the possible mechanisms of action involved in antinociception	1177:1237	The antinociceptive effect was evaluated using the formalin test and, abdominal writhing induced by acetic acid, being investigated the possible mechanisms of action involved in antinociception.
37400005	15	109	theme	industrial	2300:2309	arg1	applications					2311:2322	industrial applications	2300:2322	industrial applications	2300:2322	CONCLUSION The results obtained not only support the traditional use of the species in inflammatory conditions and pain in folk medicine but also demonstrate that this is a rich source of phytocomponents such as germacrone, which can be used as a natural and sustainable therapeutic agent with industrial applications.
35931768	6	0	theme	methane	993:999	arg1	p &lt					1013:1017	p &lt; 0.05	1013:1023	p &lt; 0.05	1013:1023	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	6	0	theme	methane	993:999	arg1	production					1001:1010	methane production	993:1010	methane production (p &lt; 0.05)	993:1024	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	5	1	theme	fiber-rich	707:716	arg1	diets					733:737	either fiber-rich or starch-rich diets	700:737	either fiber-rich or starch-rich diets	700:737	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	6	2	theme	amylolytic	891:900	arg1	bacteria					902:909	amylolytic bacteria	891:909	amylolytic bacteria	891:909	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	9	3	theme	in	1488:1489	arg1	experiments					1497:1507	Parallel in vitro experiments	1479:1507	Parallel in vitro experiments	1479:1507	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	2	4	theme	fatty	260:264	arg1	acids					266:270	volatile fatty acids	251:270	volatile fatty acids	251:270	Rumen microorganisms break down complex carbohydrates to produce volatile fatty acids and molecular hydrogen.
35931768	9	5	dep	production	1622:1631	arg1	p &lt					1634:1638	p &lt	1634:1638	p &lt; 0.05	1634:1644	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	5	6	theme	starch-rich	721:731	arg1	diets					733:737	either fiber-rich or starch-rich diets	700:737	either fiber-rich or starch-rich diets	700:737	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	10	7	theme	strategies	1878:1887	arg1	mitigation					1929:1938	methane mitigation	1921:1938	methane mitigation in ruminants	1921:1951	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	10	7	theme	strategies	1878:1887	arg1	maintenance					1904:1914	healthy rumen maintenance	1890:1914	healthy rumen maintenance	1890:1914	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	10	7	theme	strategies	1878:1887	arg1	understanding					1843:1855	understanding	1843:1855	understanding of energy harvesting strategies	1843:1887	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	6	8	theme	healthy	964:970	arg1	rumen					972:976	a healthy rumen	962:976	a healthy rumen	962:976	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	6	9	theme	starch-rich	861:871	arg1	diet					873:876	the starch-rich diet	857:876	the starch-rich diet	857:876	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	0	10	theme	Dietary	0:6	arg1	selection					8:16	Dietary selection	0:16	Dietary selection of metabolically distinct microorganisms	0:57	Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants.
35931768	7	11	theme	methanogenic	1179:1190	arg1	-hydrogenases					1208:1220	methanogenic [NiFe]- and [Fe]-hydrogenases	1179:1220	methanogenic [NiFe]- and [Fe]-hydrogenases	1179:1220	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	9	12	theme	fiber-rich	1525:1534	arg1	microbiome					1545:1554	the fiber-rich selected microbiome	1521:1554	the fiber-rich selected microbiome	1521:1554	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	10	13	from	understanding	1843:1855	arg1	ruminants					1943:1951	ruminants	1943:1951	ruminants	1943:1951	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	10	14	theme	healthy	1890:1896	arg1	maintenance					1904:1914	healthy rumen maintenance	1890:1914	healthy rumen maintenance	1890:1914	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	5	15	from	pathways	626:633	arg1	cattle					682:687	24 beef cattle	674:687	24 beef cattle adapted to either fiber-rich or starch-rich diets	674:737	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	1	16	theme	food	135:138	arg1	security					140:147	global food security	128:147	global food security	128:147	Ruminants are important for global food security but emit the greenhouse gas methane.
35931768	7	17	theme	[FeFe	1158:1162	arg1	-hydrogenases					1164:1176	increased electron-bifurcating [FeFe]-hydrogenases	1127:1176	increased electron-bifurcating [FeFe]-hydrogenases	1127:1176	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	9	18	theme	acetate	1565:1571	arg1	production					1586:1595	acetate and butyrate production	1565:1595	acetate and butyrate production	1565:1595	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	0	19	from	metabolism	75:84	arg1	ruminants					89:97	ruminants	89:97	ruminants	89:97	Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants.
35931768	7	20	theme	increased	1127:1135	arg1	-hydrogenases					1164:1176	increased electron-bifurcating [FeFe]-hydrogenases	1127:1176	increased electron-bifurcating [FeFe]-hydrogenases	1127:1176	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	2	21	theme	volatile	251:258	arg1	acids					266:270	volatile fatty acids	251:270	volatile fatty acids	251:270	Rumen microorganisms break down complex carbohydrates to produce volatile fatty acids and molecular hydrogen.
35931768	5	22	theme	metabolic	616:624	arg1	pathways					626:633	metabolic pathways	616:633	metabolic pathways	616:633	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	9	23	theme	butyrate	1577:1584	arg1	production					1586:1595	acetate and butyrate production	1565:1595	acetate and butyrate production	1565:1595	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	9	24	dep	p &lt	1634:1638	arg1	 0.05					1640:1644	 0.05	1640:1644	 0.05	1640:1644	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	9	25	dep	in	1488:1489	arg1	vitro					1491:1495	vitro	1491:1495	vitro	1491:1495	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	8	26	theme	greater	1378:1384	arg1	-hydrogenases					1419:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	2	27	theme	molecular	276:284	arg1	hydrogen					286:293	molecular hydrogen	276:293	molecular hydrogen	276:293	Rumen microorganisms break down complex carbohydrates to produce volatile fatty acids and molecular hydrogen.
35931768	9	28	theme	methane	1614:1620	arg1	production					1622:1631	methane production	1614:1631	methane production (p &lt; 0.05)	1614:1645	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	4	29	theme	hydrogen	547:554	arg1	metabolism					556:565	hydrogen metabolism	547:565	hydrogen metabolism	547:565	A better mechanistic understanding is needed on how dietary carbohydrates influence hydrogen metabolism and methanogenesis.
35931768	8	30	theme	group	1405:1409	arg1	-hydrogenases					1419:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	5	31	theme	rumen	654:658	arg1	microbiota					660:669	rumen microbiota	654:669	rumen microbiota	654:669	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	7	32	dep	lower	1252:1256	arg1	hydrogen					1268:1275	dissolved hydrogen	1258:1275	lower dissolved hydrogen (42%, p &lt; 0.001)	1252:1295	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	8	33	theme	[FeFe	1413:1417	arg1	-hydrogenases					1419:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	0	34	theme	microorganisms	44:57	arg1	selection					8:16	Dietary selection	0:16	Dietary selection of metabolically distinct microorganisms	0:57	Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants.
35931768	3	35	theme	respiratory	411:421	arg1	bacteria					423:430	hydrogenotrophic acetogenic and respiratory bacteria	379:430	hydrogenotrophic acetogenic and respiratory bacteria	379:430	This hydrogen is mainly converted into methane by archaea, but can also be used by hydrogenotrophic acetogenic and respiratory bacteria to produce useful metabolites.
35931768	8	36	theme	respiratory	1437:1447	arg1	-hydrogenases					1464:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	9	37	used	used	1752:1755	arg2	hydrogen					1719:1726	some hydrogen	1714:1726	some hydrogen that would otherwise be used by methanogenesis	1714:1773	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	7	38	theme	dissolved	1258:1266	arg1	hydrogen					1268:1275	dissolved hydrogen	1258:1275	lower dissolved hydrogen (42%, p &lt; 0.001)	1252:1295	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	4	39	theme	better	465:470	arg1	understanding					484:496	A better mechanistic understanding	463:496	A better mechanistic understanding	463:496	A better mechanistic understanding is needed on how dietary carbohydrates influence hydrogen metabolism and methanogenesis.
35931768	5	40	theme	beef	677:680	arg1	cattle					682:687	24 beef cattle	674:687	24 beef cattle adapted to either fiber-rich or starch-rich diets	674:737	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	3	41	theme	acetogenic	396:405	arg1	bacteria					423:430	hydrogenotrophic acetogenic and respiratory bacteria	379:430	hydrogenotrophic acetogenic and respiratory bacteria	379:430	This hydrogen is mainly converted into methane by archaea, but can also be used by hydrogenotrophic acetogenic and respiratory bacteria to produce useful metabolites.
35931768	8	42	theme	1d	1455:1456	arg1	-hydrogenases					1464:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	3	43	theme	useful	443:448	arg1	metabolites					450:460	useful metabolites	443:460	useful metabolites	443:460	This hydrogen is mainly converted into methane by archaea, but can also be used by hydrogenotrophic acetogenic and respiratory bacteria to produce useful metabolites.
35931768	0	44	theme	distinct	35:42	arg1	microorganisms					44:57	metabolically distinct microorganisms	21:57	metabolically distinct microorganisms	21:57	Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants.
35931768	9	45	theme	hydrogenotrophic	1677:1692	arg1	acetogens					1694:1702	the enriched hydrogenotrophic acetogens	1664:1702	the enriched hydrogenotrophic acetogens	1664:1702	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	7	46	dep	hydrogen	1268:1275	arg1	p &lt					1283:1287	p &lt	1283:1287	p &lt	1283:1287	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	7	46	dep	hydrogen	1268:1275	arg1	%					1280:1280	42%	1278:1280	42%	1278:1280	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	2	47	theme	Rumen	186:190	arg1	microorganisms					192:205	Rumen microorganisms	186:205	Rumen microorganisms	186:205	Rumen microorganisms break down complex carbohydrates to produce volatile fatty acids and molecular hydrogen.
35931768	6	48	theme	fiber	832:836	arg1	utilization					838:848	increased fiber utilization	822:848	increased fiber utilization	822:848	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	1	49	theme	global	128:133	arg1	security					140:147	global food security	128:147	global food security	128:147	Ruminants are important for global food security but emit the greenhouse gas methane.
35931768	10	50	theme	hydrogen	1796:1803	arg1	metabolism					1805:1814	hydrogen metabolism	1796:1814	hydrogen metabolism	1796:1814	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	10	51	theme	energy	1860:1865	arg1	strategies					1878:1887	energy harvesting strategies	1860:1887	energy harvesting strategies	1860:1887	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	1	52	theme	greenhouse	162:171	arg1	gas methane					173:183	emit the greenhouse gas methane	153:183	emit the greenhouse gas methane	153:183	Ruminants are important for global food security but emit the greenhouse gas methane.
35931768	2	53	theme	complex	218:224	arg1	carbohydrates					226:238	complex carbohydrates	218:238	complex carbohydrates	218:238	Rumen microorganisms break down complex carbohydrates to produce volatile fatty acids and molecular hydrogen.
35931768	7	54	theme	[Fe	1204:1206	arg1	-hydrogenases					1208:1220	methanogenic [NiFe]- and [Fe]-hydrogenases	1179:1220	methanogenic [NiFe]- and [Fe]-hydrogenases	1179:1220	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	9	55	theme	Parallel	1479:1486	arg1	experiments					1497:1507	Parallel in vitro experiments	1479:1507	Parallel in vitro experiments	1479:1507	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	6	56	theme	rumen	972:976	arg1	maintenance					947:957	the maintenance	943:957	the maintenance of a healthy rumen	943:976	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	7	57	theme	[NiFe	1192:1196	arg1	-hydrogenases					1208:1220	methanogenic [NiFe]- and [Fe]-hydrogenases	1179:1220	methanogenic [NiFe]- and [Fe]-hydrogenases	1179:1220	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	10	58	theme	harvesting	1867:1876	arg1	strategies					1878:1887	energy harvesting strategies	1860:1887	energy harvesting strategies	1860:1887	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	5	59	dep	composition	603:613	arg1	the					599:601	the	599:601	the	599:601	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	10	60	theme	rumen	1898:1902	arg1	maintenance					1904:1914	healthy rumen maintenance	1890:1914	healthy rumen maintenance	1890:1914	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	9	61	theme	selected	1536:1543	arg1	microbiome					1545:1554	the fiber-rich selected microbiome	1521:1554	the fiber-rich selected microbiome	1521:1554	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	7	62	theme	electron-bifurcating	1137:1156	arg1	-hydrogenases					1164:1176	increased electron-bifurcating [FeFe]-hydrogenases	1127:1176	increased electron-bifurcating [FeFe]-hydrogenases	1127:1176	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	5	63	from	composition	603:613	arg1	cattle					682:687	24 beef cattle	674:687	24 beef cattle adapted to either fiber-rich or starch-rich diets	674:737	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	8	64	theme	starch-rich	1315:1325	arg1	diet					1327:1330	the starch-rich diet	1311:1330	the starch-rich diet	1311:1330	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	10	65	theme	methane	1921:1927	arg1	mitigation					1929:1938	methane mitigation	1921:1938	methane mitigation in ruminants	1921:1951	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	5	66	from	activities	640:649	arg1	cattle					682:687	24 beef cattle	674:687	24 beef cattle adapted to either fiber-rich or starch-rich diets	674:737	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	7	67	theme	hydrogenotrophic	1073:1088	arg1	methanogens					1090:1100	hydrogenotrophic methanogens	1073:1100	hydrogenotrophic methanogens	1073:1100	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	4	68	theme	mechanistic	472:482	arg1	understanding					484:496	A better mechanistic understanding	463:496	A better mechanistic understanding	463:496	A better mechanistic understanding is needed on how dietary carbohydrates influence hydrogen metabolism and methanogenesis.
35931768	8	69	theme	respiratory	1345:1355	arg1	hydrogenotrophs					1357:1371	respiratory hydrogenotrophs	1345:1371	respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases	1345:1476	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	1	70	theme	emit the	153:160	arg1	gas methane					173:183	emit the greenhouse gas methane	153:183	emit the greenhouse gas methane	153:183	Ruminants are important for global food security but emit the greenhouse gas methane.
35931768	7	71	theme	fiber-rich	1044:1053	arg1	diet					1055:1058	the fiber-rich diet	1040:1058	the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase	1040:1244	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	8	72	theme	hydrogen-producing	1386:1403	arg1	-hydrogenases					1419:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	5	73	theme	microbiota	660:669	arg1	activities					640:649	activities	640:649	activities	640:649	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	5	73	theme	microbiota	660:669	arg1	composition					603:613	composition	603:613	composition	603:613	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	5	73	theme	microbiota	660:669	arg1	pathways					626:633	metabolic pathways	616:633	metabolic pathways	616:633	We profiled the composition, metabolic pathways, and activities of rumen microbiota in 24 beef cattle adapted to either fiber-rich or starch-rich diets.
35931768	10	74	from	maintenance	1904:1914	arg1	ruminants					1943:1951	ruminants	1943:1951	ruminants	1943:1951	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	3	75	theme	hydrogenotrophic	379:394	arg1	bacteria					423:430	hydrogenotrophic acetogenic and respiratory bacteria	379:430	hydrogenotrophic acetogenic and respiratory bacteria	379:430	This hydrogen is mainly converted into methane by archaea, but can also be used by hydrogenotrophic acetogenic and respiratory bacteria to produce useful metabolites.
35931768	4	76	theme	dietary	515:521	arg1	carbohydrates					523:535	dietary carbohydrates	515:535	dietary carbohydrates	515:535	A better mechanistic understanding is needed on how dietary carbohydrates influence hydrogen metabolism and methanogenesis.
35931768	8	77	theme	B	1411:1411	arg1	-hydrogenases					1419:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	6	78	theme	fibrolytic	773:782	arg1	bacteria					784:791	fibrolytic bacteria	773:791	fibrolytic bacteria	773:791	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	3	79	used	used	371:374	arg2	hydrogen					301:308	This hydrogen	296:308	This hydrogen	296:308	This hydrogen is mainly converted into methane by archaea, but can also be used by hydrogenotrophic acetogenic and respiratory bacteria to produce useful metabolites.
35931768	10	80	from	mitigation	1929:1938	arg1	ruminants					1943:1951	ruminants	1943:1951	ruminants	1943:1951	These insights into hydrogen metabolism and methanogenesis improve understanding of energy harvesting strategies, healthy rumen maintenance, and methane mitigation in ruminants.
35931768	6	81	theme	fiber-rich	744:753	arg1	diet					755:758	The fiber-rich diet	740:758	The fiber-rich diet	740:758	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	8	82	with	hydrogenotrophs	1357:1371	arg1	-hydrogenases					1464:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	8	82	with	hydrogenotrophs	1357:1371	arg1	-hydrogenases					1419:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	greater hydrogen-producing group B [FeFe]-hydrogenases	1378:1431	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	8	83	theme	group	1449:1453	arg1	-hydrogenases					1464:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	7	84	dep	diet	1055:1058	arg1	lower					1252:1256	lower	1252:1256	lower	1252:1256	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	7	85	theme	acetyl-CoA	1226:1235	arg1	synthase					1237:1244	acetyl-CoA synthase	1226:1244	acetyl-CoA synthase	1226:1244	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
35931768	9	86	theme	enriched	1668:1675	arg1	acetogens					1694:1702	the enriched hydrogenotrophic acetogens	1664:1702	the enriched hydrogenotrophic acetogens	1664:1702	Parallel in vitro experiments showed that the fiber-rich selected microbiome enhanced acetate and butyrate production while decreasing methane production (p &lt; 0.05), suggesting that the enriched hydrogenotrophic acetogens converted some hydrogen that would otherwise be used by methanogenesis.
35931768	8	87	theme	[NiFe	1458:1462	arg1	-hydrogenases					1464:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	respiratory group 1d [NiFe]-hydrogenases	1437:1476	In contrast, the starch-rich diet enriched for respiratory hydrogenotrophs with greater hydrogen-producing group B [FeFe]-hydrogenases and respiratory group 1d [NiFe]-hydrogenases.
35931768	0	88	theme	hydrogen	66:73	arg1	metabolism					75:84	hydrogen metabolism	66:84	hydrogen metabolism in ruminants	66:97	Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants.
35931768	6	89	theme	increased	822:830	arg1	utilization					838:848	increased fiber utilization	822:848	increased fiber utilization	822:848	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	6	90	theme	lactate	915:921	arg1	utilizers					923:931	lactate utilizers	915:931	lactate utilizers	915:931	The fiber-rich diet selected for fibrolytic bacteria and methanogens resulting in increased fiber utilization, while the starch-rich diet selected for amylolytic bacteria and lactate utilizers, allowing the maintenance of a healthy rumen and decreasing methane production (p &lt; 0.05).
35931768	7	91	dep	%	1280:1280	arg1	 0.001					1289:1294	 0.001	1289:1294	 0.001	1289:1294	Furthermore, the fiber-rich diet enriched for hydrogenotrophic methanogens and acetogens leading to increased electron-bifurcating [FeFe]-hydrogenases, methanogenic [NiFe]- and [Fe]-hydrogenases and acetyl-CoA synthase, with lower dissolved hydrogen (42%, p &lt; 0.001).
36531694	9	0	from	addition	791:798	arg1	weight					815:820	the molecular weight	801:820	the molecular weight	801:820	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	9	0	from	addition	791:798	arg1	distinct					899:906	distinct	899:906	distinct	899:906	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	11	1	used	used	1078:1081	arg2	microorganisms					1056:1069	these microorganisms	1050:1069	these microorganisms	1050:1069	Since these microorganisms may be used not only in known fields but also in new fields, the results of this research are expected to greatly expand the uses of hemicellulose.
36531694	2	2	from	particular	183:192	arg1	difficult					201:209	difficult	201:209	difficult	201:209	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	4	3	theme	various	498:504	arg1	regions					506:512	various regions	498:512	various regions of Okinawa Prefecture	498:534	The following four strains were selected from samples collected from various regions of Okinawa Prefecture: Kosakonia sp.
36531694	1	4	theme	biomass	102:108	arg1	utilization					110:120	biomass utilization	102:120	biomass utilization	102:120	In recent years, the importance of biomass utilization has increased, but it has not been effectively exploited.
36531694	3	5	theme	hemicellulose	331:343	arg1	utilization					316:326	the utilization	312:326	the utilization of hemicellulose	312:343	Therefore, in order to promote the utilization of hemicellulose, we screened for microorganisms capable of producing polysaccharides from D-xylose.
36531694	1	6	theme	recent	70:75	arg1	years					77:81	recent years	70:81	recent years	70:81	In recent years, the importance of biomass utilization has increased, but it has not been effectively exploited.
36531694	2	7	from	difficult	201:209	arg1	particular					183:192	particular	183:192	particular	183:192	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	11	8	dep	the	1192:1194	arg1	uses					1196:1199	uses	1196:1199	uses	1196:1199	Since these microorganisms may be used not only in known fields but also in new fields, the results of this research are expected to greatly expand the uses of hemicellulose.
36531694	6	9	dep	sp	642:643	arg1	Williamsia					631:640	Williamsia	631:640	Williamsia	631:640	(SO_006), and Williamsia sp.
36531694	2	10	theme	abundant	249:256	arg1	biopolymer					258:267	the second most abundant biopolymer	233:267	the second most abundant biopolymer of biomass	233:278	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	2	10	theme	abundant	249:256	arg1	it					195:196	it	195:196	it	195:196	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	5	11	theme	Pseudarthrobacter	595:611	arg1	sp					613:614	Pseudarthrobacter sp	595:614	Pseudarthrobacter sp	595:614	(SO_001), Papiliotrema terrestris (SO_005), Pseudarthrobacter sp.
36531694	10	12	dep	species	961:967	arg1	known					969:973	known	969:973	known to promote plant growth	969:997	The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens.
36531694	10	12	dep	species	961:967	arg1	known					1003:1007	known	1003:1007	known to suppress postharvest pathogens	1003:1041	The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens.
36531694	0	13	theme	Capable	15:21	arg1	Microorganisms					0:13	Microorganisms	0:13	Microorganisms Capable of Producing Polysaccharides from D-Xylose	0:64	Microorganisms Capable of Producing Polysaccharides from D-Xylose.
36531694	10	14	theme	selected	913:920	arg1	microorganisms					922:935	The selected microorganisms	909:935	The selected microorganisms	909:935	The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens.
36531694	9	15	theme	molecular	805:813	arg1	weight					815:820	the molecular weight	801:820	the molecular weight	801:820	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	9	15	theme	molecular	805:813	arg1	distinct					899:906	distinct	899:906	distinct	899:906	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	8	16	with	polysaccharides	749:763	arg1	shapes					780:785	different shapes	770:785	different shapes	770:785	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	4	17	theme	Kosakonia	537:545	arg1	sp					547:548	Kosakonia sp	537:548	Kosakonia sp	537:548	The following four strains were selected from samples collected from various regions of Okinawa Prefecture: Kosakonia sp.
36531694	5	18	theme	Papiliotrema	561:572	arg1	SO_001					552:557	(SO_001)	551:558	(SO_001)	551:558	(SO_001), Papiliotrema terrestris (SO_005), Pseudarthrobacter sp.
36531694	5	18	theme	Papiliotrema	561:572	arg1	terrestris					574:583	Papiliotrema terrestris (SO_005)	561:592	Papiliotrema terrestris (SO_005)	561:592	(SO_001), Papiliotrema terrestris (SO_005), Pseudarthrobacter sp.
36531694	11	19	theme	known	1095:1099	arg1	fields					1101:1106	known fields	1095:1106	known fields	1095:1106	Since these microorganisms may be used not only in known fields but also in new fields, the results of this research are expected to greatly expand the uses of hemicellulose.
36531694	2	20	dep	difficult	201:209	arg1	biopolymer					258:267	the second most abundant biopolymer	233:267	the second most abundant biopolymer of biomass	233:278	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	2	20	dep	difficult	201:209	arg1	it					195:196	it	195:196	it	195:196	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	8	21	theme	scanning	675:682	arg1	microscope					693:702	a scanning electron microscope	673:702	a scanning electron microscope (SEM)	673:708	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	8	21	theme	scanning	675:682	arg1	SEM					705:707	SEM	705:707	SEM	705:707	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	4	22	theme	following	433:441	arg1	strains					448:454	The following four strains	429:454	The following four strains	429:454	The following four strains were selected from samples collected from various regions of Okinawa Prefecture: Kosakonia sp.
36531694	2	23	theme	biomass	272:278	arg1	biopolymer					258:267	the second most abundant biopolymer	233:267	the second most abundant biopolymer of biomass	233:278	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	2	23	theme	biomass	272:278	arg1	it					195:196	it	195:196	it	195:196	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	11	24	theme	research	1152:1159	arg1	results					1136:1142	the results	1132:1142	the results of this research	1132:1159	Since these microorganisms may be used not only in known fields but also in new fields, the results of this research are expected to greatly expand the uses of hemicellulose.
36531694	5	25	dep	sp	613:614	arg1	SO_001					552:557	(SO_001)	551:558	(SO_001)	551:558	(SO_001), Papiliotrema terrestris (SO_005), Pseudarthrobacter sp.
36531694	5	25	dep	sp	613:614	arg1	terrestris					574:583	Papiliotrema terrestris (SO_005)	561:592	Papiliotrema terrestris (SO_005)	561:592	(SO_001), Papiliotrema terrestris (SO_005), Pseudarthrobacter sp.
36531694	9	26	theme	sugar	826:830	arg1	composition					832:842	sugar composition	826:842	sugar composition	826:842	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	10	27	theme	related	953:959	arg1	species					961:967	closely related species	945:967	closely related species known to promote plant growth and known to suppress postharvest pathogens	945:1041	The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens.
36531694	9	28	from	distinct	899:906	arg1	addition					791:798	addition	791:798	addition	791:798	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	8	29	theme	electron	684:691	arg1	microscope					693:702	a scanning electron microscope	673:702	a scanning electron microscope (SEM)	673:708	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	8	29	theme	electron	684:691	arg1	SEM					705:707	SEM	705:707	SEM	705:707	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	10	30	theme	plant	986:990	arg1	growth					992:997	plant growth	986:997	plant growth	986:997	The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens.
36531694	1	31	theme	utilization	110:120	arg1	importance					88:97	the importance	84:97	the importance of biomass utilization	84:120	In recent years, the importance of biomass utilization has increased, but it has not been effectively exploited.
36531694	3	32	theme	capable	377:383	arg1	microorganisms					362:375	microorganisms	362:375	microorganisms capable of producing polysaccharides from D-xylose	362:426	Therefore, in order to promote the utilization of hemicellulose, we screened for microorganisms capable of producing polysaccharides from D-xylose.
36531694	8	33	with	Observation	656:666	arg1	microscope					693:702	a scanning electron microscope	673:702	a scanning electron microscope (SEM)	673:708	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	8	33	with	Observation	656:666	arg1	SEM					705:707	SEM	705:707	SEM	705:707	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	4	34	theme	Prefecture	525:534	arg1	regions					506:512	various regions	498:512	various regions of Okinawa Prefecture	498:534	The following four strains were selected from samples collected from various regions of Okinawa Prefecture: Kosakonia sp.
36531694	9	35	theme	polysaccharides	851:865	arg1	weight					815:820	the molecular weight	801:820	the molecular weight	801:820	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	9	35	theme	polysaccharides	851:865	arg1	composition					832:842	sugar composition	826:842	sugar composition	826:842	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	9	35	theme	polysaccharides	851:865	arg1	distinct					899:906	distinct	899:906	distinct	899:906	In addition, the molecular weight and sugar composition of the polysaccharides produced by each bacterium were distinct.
36531694	10	36	theme	postharvest	1021:1031	arg1	pathogens					1033:1041	postharvest pathogens	1021:1041	postharvest pathogens	1021:1041	The selected microorganisms include closely related species known to promote plant growth and known to suppress postharvest pathogens.
36531694	2	37	theme	second	237:242	arg1	biopolymer					258:267	the second most abundant biopolymer	233:267	the second most abundant biopolymer of biomass	233:278	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	2	37	theme	second	237:242	arg1	it					195:196	it	195:196	it	195:196	In particular, it is difficult to use hemicellulose, the second most abundant biopolymer of biomass.
36531694	8	38	theme	different	770:778	arg1	shapes					780:785	different shapes	770:785	different shapes	770:785	Observation with a scanning electron microscope (SEM) confirmed that each bacterium produced polysaccharides with different shapes.
36531694	11	39	theme	new	1120:1122	arg1	fields					1124:1129	new fields	1120:1129	new fields	1120:1129	Since these microorganisms may be used not only in known fields but also in new fields, the results of this research are expected to greatly expand the uses of hemicellulose.
36531694	3	40	from	D-xylose	419:426	arg1	polysaccharides					398:412	polysaccharides	398:412	polysaccharides from D-xylose	398:426	Therefore, in order to promote the utilization of hemicellulose, we screened for microorganisms capable of producing polysaccharides from D-xylose.
36472021	0	0	theme	quality	78:84	arg1	markers					86:92	its quality markers	74:92	its quality markers	74:92	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	3	1	from	study	358:362	arg1	abroad					405:410	abroad	405:410	abroad	405:410	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	3	1	from	study	358:362	arg1	China					395:399	China	395:399	China	395:399	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	5	2	theme	quality	718:724	arg1	Q-markers					734:742	Q-markers	734:742	Q-markers	734:742	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	5	2	theme	quality	718:724	arg1	markers					726:732	quality markers	718:732	quality markers(Q-markers)	718:743	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	5	3	theme	core	702:705	arg1	concept					707:713	the core concept	698:713	the core concept of quality markers(Q-markers)	698:743	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	5	4	theme	markers	726:732	arg1	concept					707:713	the core concept	698:713	the core concept of quality markers(Q-markers)	698:743	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	0	5	from	progress	10:17	arg1	markers					86:92	its quality markers	74:92	its quality markers	74:92	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	5	6	dep	Angelicae	763:771	arg1	Radix					782:786	Angelicae Sinensis Radix	763:786	Angelicae Sinensis Radix	763:786	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	1	7	theme	edible	141:146	arg1	herb					166:169	a medicinal and edible Chinese medicinal herb	125:169	herb	166:169	Angelicae Sinensis Radix, as a medicinal and edible Chinese medicinal herb, is widely used in clinical practice.
36472021	4	8	theme	chemical	545:552	arg1	composition					554:564	chemical composition	545:564	chemical composition	545:564	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	2	9	theme	Tanchang	245:252	arg1	counties					277:284	Minxian, Tanchang, Zhangxian and Weiyuan counties	236:284	Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province	236:302	It is mainly cultivated in Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province.
36472021	3	10	theme	much	510:513	arg1	attention					515:523	much attention	510:523	much attention	510:523	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	1	11	theme	Chinese	148:154	arg1	herb					166:169	a medicinal and edible Chinese medicinal herb	125:169	herb	166:169	Angelicae Sinensis Radix, as a medicinal and edible Chinese medicinal herb, is widely used in clinical practice.
36472021	3	12	theme	recent	308:313	arg1	years					315:319	recent years	308:319	recent years	308:319	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	5	13	theme	traceability	841:852	arg1	aspects					812:818	the aspects	808:818	the aspects	808:818	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	1	14	theme	medicinal	156:164	arg1	herb					166:169	a medicinal and edible Chinese medicinal herb	125:169	herb	166:169	Angelicae Sinensis Radix, as a medicinal and edible Chinese medicinal herb, is widely used in clinical practice.
36472021	3	15	theme	comprehensive	331:343	arg1	study					358:362	the comprehensive and in-depth study	327:362	the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad	327:410	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	3	15	theme	comprehensive	331:343	arg1	composition					426:436	its chemical composition	413:436	its chemical composition	413:436	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	0	16	theme	[Research	0:8	arg1	progress					10:17	[Research progress	0:17	[Research progress of Angelicae Sinensis Radix	0:45	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	3	17	dep	Angelicae	367:375	arg1	Radix					386:390	Angelicae Sinensis Radix	367:390	Angelicae Sinensis Radix	367:390	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	2	18	theme	Weiyuan	269:275	arg1	counties					277:284	Minxian, Tanchang, Zhangxian and Weiyuan counties	236:284	Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province	236:302	It is mainly cultivated in Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province.
36472021	4	19	theme	Angelicae	627:635	arg1	effects					616:622	modern pharmacological effects	593:622	modern pharmacological effects	593:622	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	4	19	theme	Angelicae	627:635	arg1	composition					554:564	chemical composition	545:564	chemical composition	545:564	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	4	19	theme	Angelicae	627:635	arg1	efficacy					579:586	traditional efficacy	567:586	traditional efficacy	567:586	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	0	20	theme	Angelicae	22:30	arg1	analysis					62:69	predictive analysis	51:69	predictive analysis on its quality markers	51:92	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	0	20	theme	Angelicae	22:30	arg1	progress					10:17	[Research progress	0:17	[Research progress of Angelicae Sinensis Radix	0:45	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	5	21	theme	Angelicae	763:771	arg1	Q-markers					750:758	the Q-markers	746:758	the Q-markers of Angelicae Sinensis Radix	746:786	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	2	22	theme	Zhangxian	255:263	arg1	counties					277:284	Minxian, Tanchang, Zhangxian and Weiyuan counties	236:284	Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province	236:302	It is mainly cultivated in Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province.
36472021	3	23	theme	pharmacological	439:453	arg1	effects					455:461	pharmacological effects	439:461	pharmacological effects	439:461	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	5	24	theme	quality	965:971	arg1	evaluation					973:982	the quality evaluation	961:982	the quality evaluation of Angelicae Sinensis Radix	961:1010	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	4	25	theme	pharmacological	600:614	arg1	effects					616:622	modern pharmacological effects	593:622	modern pharmacological effects	593:622	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	3	26	theme	Angelicae	367:375	arg1	study					358:362	the comprehensive and in-depth study	327:362	the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad	327:410	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	3	26	theme	Angelicae	367:375	arg1	composition					426:436	its chemical composition	413:436	its chemical composition	413:436	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	5	27	theme	chemical	858:865	arg1	composition					867:877	chemical composition	858:877	chemical composition	858:877	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	4	28	theme	modern	593:598	arg1	effects					616:622	modern pharmacological effects	593:622	modern pharmacological effects	593:622	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	3	29	theme	in-depth	349:356	arg1	study					358:362	the comprehensive and in-depth study	327:362	the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad	327:410	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	3	29	theme	in-depth	349:356	arg1	composition					426:436	its chemical composition	413:436	its chemical composition	413:436	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	0	30	from	analysis	62:69	arg1	markers					86:92	its quality markers	74:92	its quality markers	74:92	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	5	31	dep	traceability	841:852	arg1	specificity					879:889	specificity	879:889	specificity	879:889	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	5	32	theme	Angelicae	987:995	arg1	evaluation					973:982	the quality evaluation	961:982	the quality evaluation of Angelicae Sinensis Radix	961:1010	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	2	33	theme	province	295:302	arg1	counties					277:284	Minxian, Tanchang, Zhangxian and Weiyuan counties	236:284	Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province	236:302	It is mainly cultivated in Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province.
36472021	5	34	theme	scientific	940:949	arg1	basis					951:955	scientific basis	940:955	scientific basis for the quality evaluation of Angelicae Sinensis Radix	940:1010	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	0	35	dep	Angelicae	22:30	arg1	Radix					41:45	Angelicae Sinensis Radix	22:45	Angelicae Sinensis Radix	22:45	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	3	36	theme	chemical	417:424	arg1	study					358:362	the comprehensive and in-depth study	327:362	the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad	327:410	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	3	36	theme	chemical	417:424	arg1	composition					426:436	its chemical composition	413:436	its chemical composition	413:436	In recent years, with the comprehensive and in-depth study of Angelicae Sinensis Radix in China and abroad, its chemical composition, pharmacological effects and application and development have attracted much attention.
36472021	1	37	theme	clinical	190:197	arg1	practice					199:206	clinical practice	190:206	clinical practice	190:206	Angelicae Sinensis Radix, as a medicinal and edible Chinese medicinal herb, is widely used in clinical practice.
36472021	4	38	dep	Angelicae	627:635	arg1	Radix					646:650	Angelicae Sinensis Radix	627:650	Angelicae Sinensis Radix	627:650	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	2	39	theme	Gansu	289:293	arg1	province					295:302	Gansu province	289:302	Gansu province	289:302	It is mainly cultivated in Minxian, Tanchang, Zhangxian and Weiyuan counties of Gansu province.
36472021	4	40	dep	composition	554:564	arg1	the					541:543	the	541:543	the	541:543	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
36472021	5	41	theme	mass	823:826	arg1	transfer					828:835	mass transfer	823:835	mass transfer	823:835	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	5	42	dep	Angelicae	987:995	arg1	Radix					1006:1010	Angelicae Sinensis Radix	987:1010	Angelicae Sinensis Radix	987:1010	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	0	43	theme	predictive	51:60	arg1	analysis					62:69	predictive analysis	51:69	predictive analysis on its quality markers	51:92	[Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers].
36472021	5	44	theme	transfer	828:835	arg1	aspects					812:818	the aspects	808:818	the aspects	808:818	On this basis, combined with the core concept of quality markers(Q-markers), the Q-markers of Angelicae Sinensis Radix were discussed from the aspects of mass transfer and traceability and chemical composition specificity, availability, and measurability, which provided scientific basis for the quality evaluation of Angelicae Sinensis Radix.
36472021	4	45	theme	traditional	567:577	arg1	efficacy					579:586	traditional efficacy	567:586	traditional efficacy	567:586	In this study, the chemical composition, traditional efficacy, and modern pharmacological effects of Angelicae Sinensis Radix were summarized.
37180713	4	0	theme	total	866:870	arg1	capacity					884:891	total antioxidant capacity	866:891	total antioxidant capacity	866:891	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	10	1	from	expression	2487:2496	arg1	cells					2508:2512	immune cells	2501:2512	immune cells	2501:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	8	2	theme	IL-2	1792:1795	arg1	secretion					1779:1787	secretion	1779:1787	secretion	1779:1787	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	2	theme	IL-2	1792:1795	arg1	expression					1764:1773	the expression	1760:1773	the expression	1760:1773	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	2	3	theme	immune-enhancing	341:356	arg1	activity					358:365	immune-enhancing activity	341:365	immune-enhancing activity	341:365	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	1	4	theme	great	245:249	arg1	potential					251:259	great potential	245:259	great potential for clinical application in immune enhancement	245:306	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	4	5	theme	capacity	884:891	arg1	activity					832:839	the scavenging activity	817:839	the scavenging activity of hydroxyl radicals and total antioxidant capacity	817:891	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	6	6	theme	immunocompromised	1175:1191	arg1	mice					1193:1196	immunocompromised mice	1175:1196	immunocompromised mice	1175:1196	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	8	7	theme	IL-6	1798:1801	arg1	secretion					1779:1787	secretion	1779:1787	secretion	1779:1787	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	7	theme	IL-6	1798:1801	arg1	expression					1764:1773	the expression	1760:1773	the expression	1760:1773	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	7	8	theme	microbiota	1388:1397	arg1	species					1350:1356	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	8	theme	microbiota	1388:1397	arg1	abundance					1371:1379	abundance	1371:1379	abundance of gut microbiota	1371:1397	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	8	theme	microbiota	1388:1397	arg1	composition					1358:1368	composition	1358:1368	composition	1358:1368	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	8	theme	microbiota	1388:1397	arg1	content					1415:1421	the altered content	1403:1421	the altered content of short-chain fatty acids in the feces	1403:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	5	9	dep	proliferation	1024:1036	arg1	the					1020:1022	the	1020:1022	the	1020:1022	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	5	9	dep	proliferation	1024:1036	arg1	ability					1055:1061	ability	1055:1061	ability	1055:1061	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	9	10	theme	intestinal	2070:2079	arg1	SCFAs					2106:2110	SCFAs	2106:2110	SCFAs	2106:2110	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	10	theme	intestinal	2070:2079	arg1	acids					2099:2103	intestinal short-chain fatty acids	2070:2103	intestinal short-chain fatty acids (SCFAs) in mice	2070:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	8	11	theme	expression	1916:1925	arg1	levels					1927:1932	IL-2, IL-6, and IFN- γ expression levels	1893:1932	IL-2, IL-6, and IFN- γ expression levels in mice	1893:1940	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	7	12	from	abundance	1371:1379	arg1	feces					1457:1461	the feces	1453:1461	the feces	1453:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	4	13	theme	radicals	853:860	arg1	activity					832:839	the scavenging activity	817:839	the scavenging activity of hydroxyl radicals and total antioxidant capacity	817:891	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	9	14	theme	fatty	2093:2097	arg1	SCFAs					2106:2110	SCFAs	2106:2110	SCFAs	2106:2110	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	14	theme	fatty	2093:2097	arg1	acids					2099:2103	intestinal short-chain fatty acids	2070:2103	intestinal short-chain fatty acids (SCFAs) in mice	2070:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	1	15	from	application	274:284	arg1	enhancement					296:306	immune enhancement	289:306	immune enhancement	289:306	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	3	16	theme	Wild	576:579	arg1	YASH1					594:598	Wild P. igniarius YASH1	576:598	Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region	576:638	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	1	17	theme	traditional	178:188	arg1	medicine					198:205	a widely used traditional Chinese medicine	164:205	a widely used traditional Chinese medicine fungus	164:212	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	7	18	theme	amplifiers	1551:1560	arg1	sequencing					1562:1571	ribosomal RNA (rRNA) amplifiers sequencing	1530:1571	ribosomal RNA (rRNA) amplifiers sequencing	1530:1571	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	2	19	theme	drugs	560:564	arg1	development					539:549	the development	535:549	the development of novel drugs	535:564	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	6	20	theme	immune	1139:1144	arg1	cells					1146:1150	immune cells	1139:1150	immune cells	1139:1150	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	6	21	theme	factor	1282:1287	arg1	expression					1203:1212	the expression	1199:1212	the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1199:1295	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	5	22	theme	cells	1073:1077	arg1	phagocytosis					1042:1053	phagocytosis	1042:1053	phagocytosis	1042:1053	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	5	22	theme	cells	1073:1077	arg1	proliferation					1024:1036	proliferation	1024:1036	proliferation	1024:1036	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	10	23	contain	have	2326:2329	arg1	Polysaccharides					2247:2261	Polysaccharides	2247:2261	Polysaccharides	2247:2261	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	23	contain	have	2326:2329	arg2	activity					2352:2359	in vitro antioxidant activity	2331:2359	in vitro antioxidant activity	2331:2359	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	23	contain	have	2326:2329	arg1	flavonoids					2267:2276	flavonoids	2267:2276	flavonoids	2267:2276	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	1	24	theme	medicine	198:205	arg1	fungus					207:212	a widely used traditional Chinese medicine fungus	164:212	a widely used traditional Chinese medicine fungus	164:212	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	1	24	theme	medicine	198:205	arg1	Introduction					100:111	Introduction	100:111	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.	100:307	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	7	25	dep	species	1350:1356	arg1	species					1350:1356	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	25	dep	species	1350:1356	arg1	abundance					1371:1379	abundance	1371:1379	abundance of gut microbiota	1371:1397	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	25	dep	species	1350:1356	arg1	composition					1358:1368	composition	1358:1368	composition	1358:1368	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	25	dep	species	1350:1356	arg1	content					1415:1421	the altered content	1403:1421	the altered content of short-chain fatty acids in the feces	1403:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	9	26	dep	composition	2180:2190	arg1	the					2168:2170	the	2168:2170	the	2168:2170	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	6	27	theme	tumor	1267:1271	arg1	TNF					1290:1292	TNF	1290:1292	TNF	1290:1292	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	6	27	theme	tumor	1267:1271	arg1	factor					1282:1287	tumor necrosis factor	1267:1287	tumor necrosis factor (TNF)	1267:1293	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	9	28	from	response	2058:2065	arg1	mice					2116:2119	mice	2116:2119	mice	2116:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	7	29	theme	RNA	1540:1542	arg1	sequencing					1562:1571	ribosomal RNA (rRNA) amplifiers sequencing	1530:1571	ribosomal RNA (rRNA) amplifiers sequencing	1530:1571	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	8	30	from	secretion	1779:1787	arg1	cells					1824:1828	immune cells	1817:1828	immune cells	1817:1828	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	6	31	theme	interleukin	1217:1227	arg1	expression					1203:1212	the expression	1199:1212	the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1199:1295	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	9	32	from	abundance	2196:2204	arg1	mice					2229:2232	mice	2229:2232	mice	2229:2232	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	5	33	theme	trypan	918:923	arg1	kit					940:942	trypan blue detection kit	918:942	trypan blue detection kit	918:942	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	10	34	from	mycelium	2302:2309	arg1	Polysaccharides					2247:2261	Polysaccharides	2247:2261	Polysaccharides	2247:2261	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	34	from	mycelium	2302:2309	arg1	flavonoids					2267:2276	flavonoids	2267:2276	flavonoids	2267:2276	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	9	35	from	composition	2180:2190	arg1	mice					2229:2232	mice	2229:2232	mice	2229:2232	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	5	36	theme	detection	930:938	arg1	kit					940:942	trypan blue detection kit	918:942	trypan blue detection kit	918:942	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	7	37	theme	drugs	1517:1521	arg1	mechanisms					1503:1512	the possible mechanisms	1490:1512	the possible mechanisms of drugs	1490:1521	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	10	38	theme	YASH1	2296:2300	arg1	mycelium					2302:2309	P. igniarius YASH1 mycelium	2283:2309	P. igniarius YASH1 mycelium	2283:2309	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	39	from	sporophore	2315:2324	arg1	Polysaccharides					2247:2261	Polysaccharides	2247:2261	Polysaccharides	2247:2261	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	39	from	sporophore	2315:2324	arg1	flavonoids					2267:2276	flavonoids	2267:2276	flavonoids	2267:2276	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	6	40	dep	interleukin	1217:1227	arg1	-α					1294:1295	-α	1294:1295	-α	1294:1295	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	10	41	theme	secretion	2452:2460	arg1	stimulation					2415:2425	stimulation	2415:2425	stimulation of IL-2, IL-6, and IFN-γ secretion	2415:2460	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	41	theme	secretion	2452:2460	arg1	promotion					2382:2390	the promotion	2378:2390	the promotion of cell proliferation	2378:2412	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	41	theme	secretion	2452:2460	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of TNF-α expression in immune cells	2467:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	0	42	theme	Phellinus	73:81	arg1	YASH1					93:97	Phellinus igniarius YASH1	73:97	Phellinus igniarius YASH1	73:97	Immune-enhancing activity of polysaccharides and flavonoids derived from Phellinus igniarius YASH1.
37180713	3	43	theme	Loess	609:613	arg1	Plateau					615:621	the Loess Plateau	605:621	the Loess Plateau in Yan'an region	605:638	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	5	44	theme	Cell	894:897	arg1	Counting					899:906	Cell Counting Kit-8	894:912	Cell Counting Kit-8	894:912	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	10	45	theme	P.	2283:2284	arg1	mycelium					2302:2309	P. igniarius YASH1 mycelium	2283:2309	P. igniarius YASH1 mycelium	2283:2309	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	46	from	stimulation	2415:2425	arg1	cells					2508:2512	immune cells	2501:2512	immune cells	2501:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	47	theme	IFN-γ	2446:2450	arg1	secretion					2452:2460	IFN-γ secretion	2446:2460	IFN-γ secretion	2446:2460	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	9	48	from	sporophore	2005:2014	arg1	flavonoids					1976:1985	flavonoids	1976:1985	flavonoids	1976:1985	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	48	from	sporophore	2005:2014	arg1	polysaccharides					1956:1970	polysaccharides	1956:1970	polysaccharides	1956:1970	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	7	49	from	composition	1358:1368	arg1	feces					1457:1461	the feces	1453:1461	the feces	1453:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	2	50	dep	activity	358:365	arg1	the					337:339	the	337:339	the	337:339	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	9	51	from	mycelium	1992:1999	arg1	flavonoids					1976:1985	flavonoids	1976:1985	flavonoids	1976:1985	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	51	from	mycelium	1992:1999	arg1	polysaccharides					1956:1970	polysaccharides	1956:1970	polysaccharides	1956:1970	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	11	52	from	YASH1	2564:2568	arg1	flavonoids					2535:2544	flavonoids	2535:2544	flavonoids	2535:2544	Polysaccharides and flavonoids from P. igniarius YASH1 may enhance immunity in immunocompromised mice and remarkably affect the intestinal flora and content of SCFAs.
37180713	11	52	from	YASH1	2564:2568	arg1	Polysaccharides					2515:2529	Polysaccharides	2515:2529	Polysaccharides	2515:2529	Polysaccharides and flavonoids from P. igniarius YASH1 may enhance immunity in immunocompromised mice and remarkably affect the intestinal flora and content of SCFAs.
37180713	8	53	theme	IL-2	1893:1896	arg1	levels					1927:1932	IL-2, IL-6, and IFN- γ expression levels	1893:1932	IL-2, IL-6, and IFN- γ expression levels in mice	1893:1940	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	4	54	theme	In	766:767	arg1	activity					787:794	In vitro antioxidant activity	766:794	In vitro antioxidant activity	766:794	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	2	55	theme	polysaccharides	400:414	arg1	activity					358:365	immune-enhancing activity	341:365	immune-enhancing activity	341:365	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	2	55	theme	polysaccharides	400:414	arg1	mechanisms					382:391	underlying mechanisms	371:391	underlying mechanisms	371:391	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	8	56	theme	IFN-	1909:1912	arg1	γ					1914:1914	IFN- γ	1909:1914	IFN- γ	1909:1914	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	10	57	dep	in	2331:2332	arg1	vitro					2334:2338	vitro	2334:2338	vitro	2334:2338	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	7	58	theme	mass	1606:1609	arg1	spectrometry					1611:1622	liquid chromatography-tandem mass spectrometry	1577:1622	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1577:1633	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	58	theme	mass	1606:1609	arg1	LC-MS/MS					1625:1632	LC-MS/MS	1625:1632	LC-MS/MS	1625:1632	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	8	59	contain	had	1717:1719	arg2	activity					1733:1740	antioxidant activity	1721:1740	antioxidant activity	1721:1740	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	59	contain	had	1717:1719	arg1	polysaccharides					1650:1664	polysaccharides	1650:1664	polysaccharides	1650:1664	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	59	contain	had	1717:1719	arg1	flavonoids					1670:1679	flavonoids	1670:1679	flavonoids	1670:1679	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	0	60	theme	igniarius	83:91	arg1	YASH1					93:97	Phellinus igniarius YASH1	73:97	Phellinus igniarius YASH1	73:97	Immune-enhancing activity of polysaccharides and flavonoids derived from Phellinus igniarius YASH1.
37180713	0	61	theme	Immune-enhancing	0:15	arg1	activity					17:24	Immune-enhancing activity	0:24	Immune-enhancing activity of polysaccharides and flavonoids	0:58	Immune-enhancing activity of polysaccharides and flavonoids derived from Phellinus igniarius YASH1.
37180713	1	62	theme	clinical	265:272	arg1	application					274:284	clinical application	265:284	clinical application in immune enhancement	265:306	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	8	63	theme	IFN-γ	1808:1812	arg1	secretion					1779:1787	secretion	1779:1787	secretion	1779:1787	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	63	theme	IFN-γ	1808:1812	arg1	expression					1764:1773	the expression	1760:1773	the expression	1760:1773	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	7	64	theme	liquid	1577:1582	arg1	spectrometry					1611:1622	liquid chromatography-tandem mass spectrometry	1577:1622	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1577:1633	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	64	theme	liquid	1577:1582	arg1	LC-MS/MS					1625:1632	LC-MS/MS	1625:1632	LC-MS/MS	1625:1632	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	10	65	theme	antioxidant	2340:2350	arg1	activity					2352:2359	in vitro antioxidant activity	2331:2359	in vitro antioxidant activity	2331:2359	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	2	66	attach	derived	431:437	arg2	polysaccharides					400:414	polysaccharides	400:414	polysaccharides	400:414	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	2	66	attach	derived	431:437	arg1	igniarius					454:462	Phellinus igniarius	444:462	Phellinus igniarius (P. igniarius)	444:477	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	9	67	theme	intestinal	2209:2218	arg1	flora					2220:2224	intestinal flora	2209:2224	intestinal flora	2209:2224	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	2	68	theme	flavonoids	420:429	arg1	activity					358:365	immune-enhancing activity	341:365	immune-enhancing activity	341:365	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	2	68	theme	flavonoids	420:429	arg1	mechanisms					382:391	underlying mechanisms	371:391	underlying mechanisms	371:391	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	10	69	theme	cell	2395:2398	arg1	proliferation					2400:2412	cell proliferation	2395:2412	cell proliferation	2395:2412	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	4	70	theme	scavenging	821:830	arg1	activity					832:839	the scavenging activity	817:839	the scavenging activity of hydroxyl radicals and total antioxidant capacity	817:891	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	7	71	theme	fatty	1438:1442	arg1	acids					1444:1448	short-chain fatty acids	1426:1448	short-chain fatty acids	1426:1448	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	6	72	theme	cytokine	1117:1124	arg1	secretion					1126:1134	cytokine secretion	1117:1134	cytokine secretion by immune cells	1117:1150	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	10	73	from	inhibition	2467:2476	arg1	cells					2508:2512	immune cells	2501:2512	immune cells	2501:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	1	74	contain	have	240:243	arg1	products					231:238	its natural products	219:238	its natural products	219:238	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	1	74	contain	have	240:243	arg2	potential					251:259	great potential	245:259	great potential for clinical application in immune enhancement	245:306	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	4	75	theme	antioxidant	775:785	arg1	activity					787:794	In vitro antioxidant activity	766:794	In vitro antioxidant activity	766:794	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	3	76	dep	Yan	626:628	arg1	region					633:638	an region	630:638	Yan'an region	626:638	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	5	77	theme	polysaccharides	986:1000	arg1	effect					968:973	the effect	964:973	the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells	964:1077	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	6	78	theme	drugs	1108:1112	arg1	effect					1094:1099	the effect	1090:1099	the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice	1090:1196	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	9	79	theme	different	2023:2031	arg1	effects					2033:2039	different effects	2023:2039	different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice	2023:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	2	80	dep	polysaccharides	400:414	arg1	the					396:398	the	396:398	the	396:398	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	2	81	theme	underlying	371:380	arg1	mechanisms					382:391	underlying mechanisms	371:391	underlying mechanisms	371:391	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	1	82	theme	natural	223:229	arg1	products					231:238	its natural products	219:238	its natural products	219:238	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	9	83	from	effects	2033:2039	arg1	response					2058:2065	the metabolic response	2044:2065	the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice	2044:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	7	84	theme	altered	1407:1413	arg1	species					1350:1356	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	84	theme	altered	1407:1413	arg1	content					1415:1421	the altered content	1403:1421	the altered content of short-chain fatty acids in the feces	1403:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	5	85	theme	flavonoids	1006:1015	arg1	effect					968:973	the effect	964:973	the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells	964:1077	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	9	86	from	mice	2116:2119	arg1	response					2058:2065	the metabolic response	2044:2065	the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice	2044:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	10	87	from	cells	2508:2512	arg1	stimulation					2415:2425	stimulation	2415:2425	stimulation of IL-2, IL-6, and IFN-γ secretion	2415:2460	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	87	from	cells	2508:2512	arg1	promotion					2382:2390	the promotion	2378:2390	the promotion of cell proliferation	2378:2412	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	87	from	cells	2508:2512	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of TNF-α expression in immune cells	2467:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	9	88	theme	metabolic	2048:2056	arg1	response					2058:2065	the metabolic response	2044:2065	the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice	2044:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	6	89	theme	interferon	1243:1252	arg1	expression					1203:1212	the expression	1199:1212	the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1199:1295	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	7	90	theme	gut	1384:1386	arg1	microbiota					1388:1397	gut microbiota	1384:1397	gut microbiota	1384:1397	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	4	91	theme	antioxidant	872:882	arg1	capacity					884:891	total antioxidant capacity	866:891	total antioxidant capacity	866:891	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	11	92	theme	immunocompromised	2594:2610	arg1	mice					2612:2615	immunocompromised mice	2594:2615	immunocompromised mice	2594:2615	Polysaccharides and flavonoids from P. igniarius YASH1 may enhance immunity in immunocompromised mice and remarkably affect the intestinal flora and content of SCFAs.
37180713	6	93	theme	IL-6	1237:1240	arg1	expression					1203:1212	the expression	1199:1212	the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α	1199:1295	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	3	94	dep	P.	581:582	arg1	igniarius					584:592	igniarius	584:592	igniarius	584:592	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	6	95	theme	immune	1156:1161	arg1	recovery					1163:1170	immune recovery	1156:1170	immune recovery in immunocompromised mice	1156:1196	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	9	96	theme	short-chain	2081:2091	arg1	SCFAs					2106:2110	SCFAs	2106:2110	SCFAs	2106:2110	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	96	theme	short-chain	2081:2091	arg1	acids					2099:2103	intestinal short-chain fatty acids	2070:2103	intestinal short-chain fatty acids (SCFAs) in mice	2070:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	8	97	theme	immune	1817:1822	arg1	cells					1824:1828	immune cells	1817:1828	immune cells	1817:1828	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	1	98	theme	used	173:176	arg1	medicine					198:205	a widely used traditional Chinese medicine	164:205	a widely used traditional Chinese medicine fungus	164:212	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	4	99	theme	hydroxyl	844:851	arg1	radicals					853:860	hydroxyl radicals	844:860	hydroxyl radicals	844:860	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	11	100	from	immunity	2582:2589	arg1	mice					2612:2615	immunocompromised mice	2594:2615	immunocompromised mice	2594:2615	Polysaccharides and flavonoids from P. igniarius YASH1 may enhance immunity in immunocompromised mice and remarkably affect the intestinal flora and content of SCFAs.
37180713	9	101	theme	acids	2099:2103	arg1	response					2058:2065	the metabolic response	2044:2065	the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice	2044:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	1	102	theme	Chinese	190:196	arg1	medicine					198:205	a widely used traditional Chinese medicine	164:205	a widely used traditional Chinese medicine fungus	164:212	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	2	103	theme	novel	554:558	arg1	drugs					560:564	novel drugs	554:564	novel drugs	554:564	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	11	104	theme	SCFAs	2675:2679	arg1	content					2664:2670	content	2664:2670	content of SCFAs	2664:2679	Polysaccharides and flavonoids from P. igniarius YASH1 may enhance immunity in immunocompromised mice and remarkably affect the intestinal flora and content of SCFAs.
37180713	11	104	theme	SCFAs	2675:2679	arg1	flora					2654:2658	the intestinal flora	2639:2658	the intestinal flora	2639:2658	Polysaccharides and flavonoids from P. igniarius YASH1 may enhance immunity in immunocompromised mice and remarkably affect the intestinal flora and content of SCFAs.
37180713	6	105	theme	necrosis	1273:1280	arg1	TNF					1290:1292	TNF	1290:1292	TNF	1290:1292	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	6	105	theme	necrosis	1273:1280	arg1	factor					1282:1287	tumor necrosis factor	1267:1287	tumor necrosis factor (TNF)	1267:1293	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	7	106	theme	ribosomal	1530:1538	arg1	rRNA					1545:1548	rRNA	1545:1548	rRNA	1545:1548	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	106	theme	ribosomal	1530:1538	arg1	RNA					1540:1542	ribosomal RNA	1530:1542	ribosomal RNA (rRNA) amplifiers sequencing	1530:1571	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	10	107	from	promotion	2382:2390	arg1	cells					2508:2512	immune cells	2501:2512	immune cells	2501:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	108	theme	proliferation	2400:2412	arg1	stimulation					2415:2425	stimulation	2415:2425	stimulation of IL-2, IL-6, and IFN-γ secretion	2415:2460	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	108	theme	proliferation	2400:2412	arg1	promotion					2382:2390	the promotion	2378:2390	the promotion of cell proliferation	2378:2412	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	108	theme	proliferation	2400:2412	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of TNF-α expression in immune cells	2467:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	9	109	from	use	2130:2132	arg1	response					2058:2065	the metabolic response	2044:2065	the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice	2044:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	3	110	theme	P.	581:582	arg1	YASH1					594:598	Wild P. igniarius YASH1	576:598	Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region	576:638	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	7	111	from	content	1415:1421	arg1	feces					1457:1461	the feces	1453:1461	the feces	1453:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	10	112	theme	IL-6	2436:2439	arg1	stimulation					2415:2425	stimulation	2415:2425	stimulation of IL-2, IL-6, and IFN-γ secretion	2415:2460	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	112	theme	IL-6	2436:2439	arg1	promotion					2382:2390	the promotion	2378:2390	the promotion of cell proliferation	2378:2412	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	112	theme	IL-6	2436:2439	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of TNF-α expression in immune cells	2467:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	5	113	theme	blue	925:928	arg1	kit					940:942	trypan blue detection kit	918:942	trypan blue detection kit	918:942	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	10	114	theme	TNF-α	2481:2485	arg1	expression					2487:2496	TNF-α expression	2481:2496	TNF-α expression in immune cells	2481:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	115	theme	IL-2	2430:2433	arg1	stimulation					2415:2425	stimulation	2415:2425	stimulation of IL-2, IL-6, and IFN-γ secretion	2415:2460	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	115	theme	IL-2	2430:2433	arg1	promotion					2382:2390	the promotion	2378:2390	the promotion of cell proliferation	2378:2412	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	115	theme	IL-2	2430:2433	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of TNF-α expression in immune cells	2467:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	6	116	theme	cellular	1318:1325	arg1	levels					1338:1343	the cellular and animal levels	1314:1343	the cellular and animal levels	1314:1343	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	5	117	theme	immune	1066:1071	arg1	cells					1073:1077	immune cells	1066:1077	immune cells	1066:1077	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	4	118	dep	In	766:767	arg1	vitro					769:773	vitro	769:773	vitro	769:773	In vitro antioxidant activity was detected through the scavenging activity of hydroxyl radicals and total antioxidant capacity.
37180713	7	119	theme	possible	1494:1501	arg1	mechanisms					1503:1512	the possible mechanisms	1490:1512	the possible mechanisms of drugs	1490:1521	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	2	120	theme	theoretical	496:506	arg1	basis					525:529	a theoretical and experimental basis	494:529	a theoretical and experimental basis for the development of novel drugs	494:564	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	1	121	dep	igniarius	124:132	arg1	igniarius					138:146	P. igniarius	135:146	P. igniarius	135:146	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	8	122	theme	IL-6	1899:1902	arg1	levels					1927:1932	IL-2, IL-6, and IFN- γ expression levels	1893:1932	IL-2, IL-6, and IFN- γ expression levels in mice	1893:1940	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	123	theme	antioxidant	1721:1731	arg1	activity					1733:1740	antioxidant activity	1721:1740	antioxidant activity	1721:1740	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	9	124	dep	showed	2016:2021	arg1	changed					2160:2166	changed	2160:2166	showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice	2016:2232	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	8	125	from	levels	1927:1932	arg1	mice					1937:1940	mice	1937:1940	mice	1937:1940	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	126	from	expression	1764:1773	arg1	cells					1824:1828	immune cells	1817:1828	immune cells	1817:1828	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	9	127	theme	drugs	2143:2147	arg1	use					2130:2132	the use	2126:2132	the use of these drugs	2126:2147	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	127	theme	drugs	2143:2147	arg1	effects					2033:2039	different effects	2023:2039	different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice	2023:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	128	from	acids	2099:2103	arg1	mice					2116:2119	mice	2116:2119	mice	2116:2119	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	11	129	theme	intestinal	2643:2652	arg1	flora					2654:2658	the intestinal flora	2639:2658	the intestinal flora	2639:2658	Polysaccharides and flavonoids from P. igniarius YASH1 may enhance immunity in immunocompromised mice and remarkably affect the intestinal flora and content of SCFAs.
37180713	6	130	from	recovery	1163:1170	arg1	mice					1193:1196	immunocompromised mice	1175:1196	immunocompromised mice	1175:1196	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	8	131	theme	γ	1914:1914	arg1	levels					1927:1932	IL-2, IL-6, and IFN- γ expression levels	1893:1932	IL-2, IL-6, and IFN- γ expression levels in mice	1893:1940	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	2	132	dep	igniarius	454:462	arg1	igniarius					468:476	P. igniarius	465:476	P. igniarius	465:476	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	2	133	theme	experimental	512:523	arg1	basis					525:529	a theoretical and experimental basis	494:529	a theoretical and experimental basis for the development of novel drugs	494:564	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	3	134	from	Plateau	615:621	arg1	YASH1					594:598	Wild P. igniarius YASH1	576:598	Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region	576:638	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	3	134	from	Plateau	615:621	arg1	Yan					626:628	Yan	626:628	Yan	626:628	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	5	135	used	used	949:952	arg2	kit					940:942	trypan blue detection kit	918:942	trypan blue detection kit	918:942	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	5	135	used	used	949:952	arg2	Counting					899:906	Cell Counting Kit-8	894:912	Cell Counting Kit-8	894:912	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	8	136	theme	TNF-α	1847:1851	arg1	expression					1853:1862	TNF-α expression	1847:1862	TNF-α expression	1847:1862	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	137	attach	derived	1681:1687	arg1	mycelium					1694:1701	mycelium	1694:1701	mycelium	1694:1701	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	137	attach	derived	1681:1687	arg2	polysaccharides					1650:1664	polysaccharides	1650:1664	polysaccharides	1650:1664	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	8	137	attach	derived	1681:1687	arg1	sporophore					1706:1715	sporophore	1706:1715	sporophore	1706:1715	Results: Both polysaccharides and flavonoids derived from mycelium or sporophore had antioxidant activity and may stimulate the expression and secretion of IL-2, IL-6, and IFN-γ in immune cells while inhibiting TNF-α expression and secretion and increasing IL-2, IL-6, and IFN- γ expression levels in mice.
37180713	10	138	theme	immune	2501:2506	arg1	cells					2508:2512	immune cells	2501:2512	immune cells	2501:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	0	139	theme	polysaccharides	29:43	arg1	activity					17:24	Immune-enhancing activity	0:24	Immune-enhancing activity of polysaccharides and flavonoids	0:58	Immune-enhancing activity of polysaccharides and flavonoids derived from Phellinus igniarius YASH1.
37180713	1	140	theme	Phellinus	114:122	arg1	Sanghuang					150:158	Sanghuang	150:158	Sanghuang	150:158	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	1	140	theme	Phellinus	114:122	arg1	igniarius					124:132	Phellinus igniarius	114:132	Phellinus igniarius (P. igniarius) (Sanghuang)	114:159	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	7	141	theme	chromatography-tandem	1584:1604	arg1	spectrometry					1611:1622	liquid chromatography-tandem mass spectrometry	1577:1622	liquid chromatography-tandem mass spectrometry (LC-MS/MS)	1577:1633	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	141	theme	chromatography-tandem	1584:1604	arg1	LC-MS/MS					1625:1632	LC-MS/MS	1625:1632	LC-MS/MS	1625:1632	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	2	142	theme	Phellinus	444:452	arg1	igniarius					454:462	Phellinus igniarius	444:462	Phellinus igniarius (P. igniarius)	444:477	This study aimed to explore the immune-enhancing activity and underlying mechanisms of the polysaccharides and flavonoids derived from Phellinus igniarius (P. igniarius) and to provide a theoretical and experimental basis for the development of novel drugs.
37180713	10	143	theme	expression	2487:2496	arg1	stimulation					2415:2425	stimulation	2415:2425	stimulation of IL-2, IL-6, and IFN-γ secretion	2415:2460	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	143	theme	expression	2487:2496	arg1	promotion					2382:2390	the promotion	2378:2390	the promotion of cell proliferation	2378:2412	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	10	143	theme	expression	2487:2496	arg1	inhibition					2467:2476	inhibition	2467:2476	inhibition of TNF-α expression in immune cells	2467:2512	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	0	144	theme	flavonoids	49:58	arg1	activity					17:24	Immune-enhancing activity	0:24	Immune-enhancing activity of polysaccharides and flavonoids	0:58	Immune-enhancing activity of polysaccharides and flavonoids derived from Phellinus igniarius YASH1.
37180713	1	145	theme	immune	289:294	arg1	enhancement					296:306	immune enhancement	289:306	immune enhancement	289:306	Introduction: Phellinus igniarius (P. igniarius) (Sanghuang) is a widely used traditional Chinese medicine fungus, and its natural products have great potential for clinical application in immune enhancement.
37180713	9	146	theme	flora	2220:2224	arg1	composition					2180:2190	species composition	2172:2190	species composition	2172:2190	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	9	146	theme	flora	2220:2224	arg1	abundance					2196:2204	abundance	2196:2204	abundance	2196:2204	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	6	147	theme	animal	1331:1336	arg1	levels					1338:1343	the cellular and animal levels	1314:1343	the cellular and animal levels	1314:1343	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	10	148	dep	P.	2283:2284	arg1	igniarius					2286:2294	igniarius	2286:2294	igniarius	2286:2294	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	6	149	from	secretion	1126:1134	arg1	mice					1193:1196	immunocompromised mice	1175:1196	immunocompromised mice	1175:1196	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	7	150	theme	acids	1444:1448	arg1	species					1350:1356	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces	1346:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	150	theme	acids	1444:1448	arg1	abundance					1371:1379	abundance	1371:1379	abundance of gut microbiota	1371:1397	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	150	theme	acids	1444:1448	arg1	composition					1358:1368	composition	1358:1368	composition	1358:1368	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	7	150	theme	acids	1444:1448	arg1	content					1415:1421	the altered content	1403:1421	the altered content of short-chain fatty acids in the feces	1403:1461	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	5	151	theme	extract	978:984	arg1	polysaccharides					986:1000	extract polysaccharides	978:1000	extract polysaccharides	978:1000	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	6	152	from	effect	1094:1099	arg1	recovery					1163:1170	immune recovery	1156:1170	immune recovery in immunocompromised mice	1156:1196	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	6	152	from	effect	1094:1099	arg1	secretion					1126:1134	cytokine secretion	1117:1134	cytokine secretion by immune cells	1117:1150	To assess the effect of the drugs on cytokine secretion by immune cells and immune recovery in immunocompromised mice, the expression of interleukin (IL)-2, IL-6, interferon (IFN)-γ, and tumor necrosis factor (TNF)-α were examined at the cellular and animal levels.
37180713	7	153	theme	short-chain	1426:1436	arg1	acids					1444:1448	short-chain fatty acids	1426:1448	short-chain fatty acids	1426:1448	The species composition, abundance of gut microbiota and the altered content of short-chain fatty acids in the feces were analyzed to elucidate the possible mechanisms of drugs by 16S ribosomal RNA (rRNA) amplifiers sequencing and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
37180713	10	154	theme	in	2331:2332	arg1	activity					2352:2359	in vitro antioxidant activity	2331:2359	in vitro antioxidant activity	2331:2359	Discussion: Polysaccharides and flavonoids from P. igniarius YASH1 mycelium and sporophore have in vitro antioxidant activity, and they affect the promotion of cell proliferation, stimulation of IL-2, IL-6, and IFN-γ secretion, and inhibition of TNF-α expression in immune cells.
37180713	9	155	theme	species	2172:2178	arg1	composition					2180:2190	species composition	2172:2190	species composition	2172:2190	Furthermore, polysaccharides and flavonoids from mycelium and sporophore showed different effects on the metabolic response of intestinal short-chain fatty acids (SCFAs) in mice, and the use of these drugs remarkably changed the species composition and abundance of intestinal flora in mice.
37180713	3	156	theme	total	679:683	arg1	flavonoids					685:694	total flavonoids	679:694	total flavonoids	679:694	Methods: Wild P. igniarius YASH1 from the Loess Plateau in Yan'an region was collected, and polysaccharides and total flavonoids were extracted, isolated and identified from mycelium and sporophore.
37180713	5	157	from	effect	968:973	arg1	phagocytosis					1042:1053	phagocytosis	1042:1053	phagocytosis	1042:1053	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
37180713	5	157	from	effect	968:973	arg1	proliferation					1024:1036	proliferation	1024:1036	proliferation	1024:1036	Cell Counting Kit-8 and trypan blue detection kit were used to detect the effect of extract polysaccharides and flavonoids on the proliferation and phagocytosis ability of immune cells.
35312878	2	0	from	rhizome	177:183	arg1	Polysaccharides					152:166	Polysaccharides	152:166	Polysaccharides from the rhizome of Polygonatum sibiricum	152:208	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	0	1	from	the	21:23	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from the	0:23	Polysaccharides from the leaves of Polygonatum sibiricum Red.
35312878	4	2	theme	6.6:15.4:4.5:8.8:40.7:24	809:832	arg1	ratios					799:804	molar ratios	793:804	molar ratios of 6.6:15.4:4.5:8.8:40.7:24	793:832	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	3	3	from	the	442:444	arg1	polysaccharides					421:435	crude polysaccharides	415:435	crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs)	415:626	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	8	4	theme	PsPs	1259:1262	arg1	group					1264:1268	the PsPs group	1255:1268	the PsPs group	1255:1268	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	3	5	from	effects	522:528	arg1	microbes					583:590	intestinal microbes	572:590	intestinal microbes	572:590	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	3	5	from	effects	522:528	arg1	SCFAs					621:625	SCFAs	621:625	SCFAs	621:625	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	3	5	from	effects	522:528	arg1	acids					614:618	short-chain fatty acids	596:618	short-chain fatty acids (SCFAs)	596:626	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	5	6	from	effects	853:859	arg1	microflora					883:892	intestinal microflora	872:892	intestinal microflora in mice	872:900	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	8	7	theme	SCFAs	1246:1250	arg1	Levels					1236:1241	Levels	1236:1241	Levels of SCFAs in the PsPs group	1236:1268	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	2	8	from	the	334:336	arg1	polysaccharides					313:327	the polysaccharides	309:327	the polysaccharides from the leaves of P. sibiricum	309:359	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	8	9	theme	control	1313:1319	arg1	acid					1378:1381	butyric acid	1370:1381	butyric acid	1370:1381	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	8	9	theme	control	1313:1319	arg1	acid					1344:1347	acetic acid	1337:1347	acetic acid	1337:1347	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	8	9	theme	control	1313:1319	arg1	acid					1360:1363	propionic acid	1350:1363	propionic acid	1350:1363	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	8	9	theme	control	1313:1319	arg1	mice					1321:1324	control mice	1313:1324	control mice	1313:1324	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	8	10	theme	propionic	1350:1358	arg1	acid					1360:1363	propionic acid	1350:1363	propionic acid	1350:1363	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	2	11	theme	microbiota	293:302	arg1	regulation					268:277	the regulation	264:277	the regulation of intestinal microbiota	264:302	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	6	12	theme	Lactobacillus	1079:1091	arg1	abundance					1066:1074	The abundance	1062:1074	The abundance of Lactobacillus	1062:1091	The abundance of Lactobacillus increased, while those of Lachnospiraceae and Bacteroides reduced (at the genus level) by PsPs treatment.
35312878	2	13	theme	intestinal	282:291	arg1	microbiota					293:302	intestinal microbiota	282:302	intestinal microbiota	282:302	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	3	14	theme	P.	533:534	arg1	polysaccharides					546:560	P. sibiricum polysaccharides	533:560	P. sibiricum polysaccharides (PsPs)	533:567	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	3	14	theme	P.	533:534	arg1	PsPs					563:566	PsPs	563:566	PsPs	563:566	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	5	15	theme	PsPs	864:867	arg1	effects					853:859	The effects	849:859	The effects of PsPs on intestinal microflora in mice	849:900	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	4	16	theme	galacturonic	737:748	arg1	acid					750:753	galacturonic acid	737:753	galacturonic acid	737:753	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	5	17	from	decrease	1015:1022	arg1	abundance					985:993	the relative abundance	972:993	the relative abundance of Firmicutes	972:1007	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	5	18	theme	phylum	1048:1053	arg1	level					1055:1059	the phylum level	1044:1059	the phylum level	1044:1059	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	1	19	theme	gut	75:77	arg1	microbiota					79:88	the gut microbiota	71:88	the gut microbiota	71:88	regulate the gut microbiota and affect the production of short-chain fatty acids in mice.
35312878	6	20	theme	PsPs	1183:1186	arg1	treatment					1188:1196	PsPs treatment	1183:1196	PsPs treatment	1183:1196	The abundance of Lactobacillus increased, while those of Lachnospiraceae and Bacteroides reduced (at the genus level) by PsPs treatment.
35312878	5	21	from	microflora	883:892	arg1	mice					897:900	mice	897:900	mice	897:900	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	2	22	dep	the	334:336	arg1	leaves					338:343	leaves	338:343	leaves	338:343	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	3	23	theme	crude	415:419	arg1	polysaccharides					421:435	crude polysaccharides	415:435	crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs)	415:626	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	4	24	theme	molar	793:797	arg1	ratios					799:804	molar ratios	793:804	molar ratios of 6.6:15.4:4.5:8.8:40.7:24	793:832	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	1	25	from	production	105:114	arg1	mice					146:149	mice	146:149	mice	146:149	regulate the gut microbiota and affect the production of short-chain fatty acids in mice.
35312878	4	26	theme	monosaccharide	680:693	arg1	composition					695:705	a monosaccharide composition	678:705	a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose	678:785	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	5	27	theme	relative	976:983	arg1	abundance					985:993	the relative abundance	972:993	the relative abundance of Firmicutes	972:1007	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	3	28	theme	sibiricum	536:544	arg1	polysaccharides					546:560	P. sibiricum polysaccharides	533:560	P. sibiricum polysaccharides (PsPs)	533:567	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	3	28	theme	sibiricum	536:544	arg1	PsPs					563:566	PsPs	563:566	PsPs	563:566	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	3	29	theme	intestinal	572:581	arg1	microbes					583:590	intestinal microbes	572:590	intestinal microbes	572:590	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	8	30	theme	butyric	1370:1376	arg1	acid					1378:1381	butyric acid	1370:1381	butyric acid	1370:1381	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	3	31	theme	polysaccharides	546:560	arg1	effects					522:528	the effects	518:528	the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs)	518:626	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	5	32	from	increase	960:967	arg1	abundance					985:993	the relative abundance	972:993	the relative abundance of Firmicutes	972:1007	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	4	33	theme	sugar	650:654	arg1	content					656:662	a total sugar content	642:662	a total sugar content of 97.48%	642:672	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	2	34	theme	biological	231:240	arg1	regulation					268:277	the regulation	264:277	the regulation of intestinal microbiota	264:302	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	2	34	theme	biological	231:240	arg1	activities					242:251	biological activities	231:251	biological activities	231:251	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	8	35	theme	acetic	1337:1342	arg1	acid					1344:1347	acetic acid	1337:1347	acetic acid	1337:1347	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	6	36	dep	reduced	1151:1157	arg1	level					1173:1177	the genus level	1163:1177	the genus level	1163:1177	The abundance of Lactobacillus increased, while those of Lachnospiraceae and Bacteroides reduced (at the genus level) by PsPs treatment.
35312878	6	37	theme	genus	1167:1171	arg1	level					1173:1177	the genus level	1163:1177	the genus level	1163:1177	The abundance of Lactobacillus increased, while those of Lachnospiraceae and Bacteroides reduced (at the genus level) by PsPs treatment.
35312878	4	38	theme	%	672:672	arg1	content					656:662	a total sugar content	642:662	a total sugar content of 97.48%	642:672	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	4	38	theme	%	672:672	arg1	composition					695:705	a monosaccharide composition	678:705	a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose	678:785	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	4	39	contain	had	638:640	arg1	PsPs					633:636	The PsPs	629:636	The PsPs	629:636	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	4	39	contain	had	638:640	arg2	composition					695:705	a monosaccharide composition	678:705	a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose	678:785	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	4	39	contain	had	638:640	arg2	content					656:662	a total sugar content	642:662	a total sugar content of 97.48%	642:672	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
35312878	3	40	dep	the	442:444	arg1	leaves					446:451	leaves	446:451	leaves	446:451	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	7	41	theme	microbes	1218:1225	arg1	composition					1203:1213	The composition	1199:1213	The composition of microbes	1199:1225	The composition of microbes changed.
35312878	2	42	theme	activities	242:251	arg1	regulation					268:277	the regulation	264:277	the regulation of intestinal microbiota	264:302	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	2	42	theme	activities	242:251	arg1	variety					220:226	a variety	218:226	a variety of biological activities, including the regulation of intestinal microbiota,	218:303	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	2	42	theme	activities	242:251	arg1	activities					242:251	biological activities	231:251	biological activities	231:251	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	2	43	theme	sibiricum	200:208	arg1	rhizome					177:183	the rhizome	173:183	the rhizome of Polygonatum sibiricum	173:208	Polysaccharides from the rhizome of Polygonatum sibiricum display a variety of biological activities, including the regulation of intestinal microbiota, but the polysaccharides from the leaves of P. sibiricum have not been studied extensively.
35312878	3	44	theme	fatty	608:612	arg1	SCFAs					621:625	SCFAs	621:625	SCFAs	621:625	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	3	44	theme	fatty	608:612	arg1	acids					614:618	short-chain fatty acids	596:618	short-chain fatty acids (SCFAs)	596:626	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	8	45	from	Levels	1236:1241	arg1	group					1264:1268	the PsPs group	1255:1268	the PsPs group	1255:1268	Levels of SCFAs in the PsPs group were significantly increased compared with control mice, including acetic acid, propionic acid, and butyric acid.
35312878	1	46	theme	short-chain	119:129	arg1	acids					137:141	short-chain fatty acids	119:141	short-chain fatty acids	119:141	regulate the gut microbiota and affect the production of short-chain fatty acids in mice.
35312878	5	47	theme	16S	926:928	arg1	results					941:947	16S sequencing results	926:947	16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level	926:1059	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	5	48	theme	Firmicutes	998:1007	arg1	abundance					985:993	the relative abundance	972:993	the relative abundance of Firmicutes	972:1007	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	9	49	theme	intestinal	1454:1463	arg1	probiotics					1471:1480	the intestinal tract probiotics	1450:1480	the intestinal tract probiotics	1450:1480	These results suggest that PsPs can act as prebiotics, regulating the intestinal tract probiotics.
35312878	1	50	theme	fatty	131:135	arg1	acids					137:141	short-chain fatty acids	119:141	short-chain fatty acids	119:141	regulate the gut microbiota and affect the production of short-chain fatty acids in mice.
35312878	5	51	theme	intestinal	872:881	arg1	microflora					883:892	intestinal microflora	872:892	intestinal microflora in mice	872:900	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	1	52	theme	acids	137:141	arg1	production					105:114	the production	101:114	the production of short-chain fatty acids in mice	101:149	regulate the gut microbiota and affect the production of short-chain fatty acids in mice.
35312878	9	53	theme	tract	1465:1469	arg1	probiotics					1471:1480	the intestinal tract probiotics	1450:1480	the intestinal tract probiotics	1450:1480	These results suggest that PsPs can act as prebiotics, regulating the intestinal tract probiotics.
35312878	5	54	theme	sequencing	930:939	arg1	results					941:947	16S sequencing results	926:947	16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level	926:1059	The effects of PsPs on intestinal microflora in mice were also studied, with 16S sequencing results showing an increase in the relative abundance of Firmicutes and a decrease in Bacteroidetes at the phylum level.
35312878	3	55	theme	short-chain	596:606	arg1	SCFAs					621:625	SCFAs	621:625	SCFAs	621:625	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	3	55	theme	short-chain	596:606	arg1	acids					614:618	short-chain fatty acids	596:618	short-chain fatty acids (SCFAs)	596:626	Here, we extracted crude polysaccharides from the leaves of P. sibiricum and further separated and purified them to study the effects of P. sibiricum polysaccharides (PsPs) on intestinal microbes and short-chain fatty acids (SCFAs).
35312878	4	56	theme	total	644:648	arg1	content					656:662	a total sugar content	642:662	a total sugar content of 97.48%	642:672	The PsPs had a total sugar content of 97.48% and a monosaccharide composition comprising mannose, rhamnose, galacturonic acid, glucose, xylose, and arabinose, with molar ratios of 6.6:15.4:4.5:8.8:40.7:24, respectively.
37097162	15	0	with	bacteria	2494:2501	arg1	genes					2508:2512	genes	2508:2512	genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy	2508:2676	It points to keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy.
37097162	6	1	theme	carbohydrate	1063:1074	arg1	decomposition					1076:1088	carbohydrate decomposition	1063:1088	carbohydrate decomposition	1063:1088	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	2	2	theme	bacterial	347:355	arg1	community					357:365	the gut bacterial community	339:365	the gut bacterial community	339:365	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	8	3	from	bacteria	1335:1342	arg1	intestine					1363:1371	intestine	1363:1371	intestine	1363:1371	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	8	3	from	bacteria	1335:1342	arg1	stomach					1351:1357	stomach	1351:1357	stomach	1351:1357	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	2	4	from	arrangement	324:334	arg1	community					357:365	the gut bacterial community	339:365	the gut bacterial community	339:365	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	3	5	theme	genes	716:720	arg1	prediction					685:694	the prediction	681:694	the prediction of their functional genes	681:720	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	14	6	theme	sea	2333:2335	arg1	urchin					2337:2342	the sea urchin	2329:2342	the sea urchin's gut microbiome ecology	2329:2367	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	3	7	theme	bacterial	544:552	arg1	communities					554:564	the bacterial communities	540:564	the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins	540:629	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	15	8	theme	complex	2560:2566	arg1	polysaccharides					2577:2591	the complex sulfated polysaccharides	2556:2591	the complex sulfated polysaccharides	2556:2591	It points to keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy.
37097162	1	9	theme	fiber-rich	265:274	arg1	seaweed					276:282	fiber-rich seaweed	265:282	fiber-rich seaweed	265:282	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	9	10	theme	algae	1630:1634	arg1	degradation					1590:1600	the degradation	1586:1600	the degradation of polysaccharides of other algae and plants	1586:1645	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	6	11	theme	various	1147:1153	arg1	ways					1155:1158	various ways	1147:1158	various ways of N and S metabolism	1147:1180	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	12	12	theme	alga	2076:2079	arg1	digestion					2081:2089	alga digestion	2076:2089	alga digestion	2076:2089	This research was driven by the need to expand the knowledge of bacteria that may aid this host in alga digestion and their phylogeny, roles, and activity niches.
37097162	6	13	from	stomach	1014:1020	arg1	common					1000:1005	common	1000:1005	common	1000:1005	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	14	14	theme	distinct	2394:2401	arg1	communities					2413:2423	distinct bacterial communities	2394:2423	distinct bacterial communities	2394:2423	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	9	15	theme	plants	1640:1645	arg1	degradation					1590:1600	the degradation	1586:1600	the degradation of polysaccharides of other algae and plants	1586:1645	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	7	16	from	phyla	1235:1239	arg1	gut					1248:1250	the gut	1244:1250	the gut	1244:1250	Bacteroidetes and Firmicutes were found as the main phyla in the gut and are presumably also necessary in food digestion.
37097162	6	17	from	intestine	1026:1034	arg1	common					1000:1005	common	1000:1005	common	1000:1005	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	10	18	theme	microbial	1730:1738	arg1	communities					1740:1750	microbial communities	1730:1750	microbial communities	1730:1750	We conclude that the succession of microbial communities along the gut obtained supports the hypothesis on bacterial contribution to food digestion.
37097162	6	19	theme	fatty	1130:1134	arg1	acids					1136:1140	short-chain fatty acids	1118:1140	short-chain fatty acids	1118:1140	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	8	20	from	genera	1382:1387	arg1	bacteria					1335:1342	The abundant sulfate-reducing bacteria	1305:1342	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea	1305:1435	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	13	21	theme	potential	2254:2262	arg1	contribution					2264:2275	the potential contribution	2250:2275	the potential contribution to food digestion	2250:2293	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	1	22	theme	Dietary	110:116	arg1	influence					118:126	Dietary influence	110:126	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis	110:209	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	15	23	theme	keynote	2486:2492	arg1	bacteria					2494:2501	keynote bacteria	2486:2501	keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy	2486:2676	It points to keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy.
37097162	11	24	theme	nutrient	1930:1937	arg1	recycling					1939:1947	nutrient recycling	1930:1947	nutrient recycling	1930:1947	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37097162	9	25	theme	urchin	1672:1677	arg1	microbiome					1683:1692	this sea urchin gut microbiome	1663:1692	this sea urchin gut microbiome	1663:1692	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	16	26	theme	genomic	2698:2704	arg1	arsenal					2706:2712	The gut bacteria's genomic arsenal	2679:2712	The gut bacteria's genomic arsenal	2679:2712	The gut bacteria's genomic arsenal may also help to gain energy from diets of other algae and plants.
37097162	9	27	located	present	1652:1658	arg1	microbiome					1683:1692	this sea urchin gut microbiome	1663:1692	this sea urchin gut microbiome	1663:1692	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	9	27	located	present	1652:1658	arg2	genes					1576:1580	genes	1576:1580	genes for the degradation of polysaccharides of other algae and plants	1576:1645	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	11	28	theme	constructing	1953:1964	arg1	reefs					1970:1974	constructing new reefs	1953:1974	constructing new reefs	1953:1974	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37097162	6	29	theme	metabolism	1171:1180	arg1	decomposition					1076:1088	carbohydrate decomposition	1063:1088	carbohydrate decomposition	1063:1088	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	29	theme	metabolism	1171:1180	arg1	ways					1155:1158	various ways	1147:1158	various ways of N and S metabolism	1147:1180	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	29	theme	metabolism	1171:1180	arg1	synthesis					1105:1113	synthesis	1105:1113	synthesis of short-chain fatty acids	1105:1140	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	29	theme	metabolism	1171:1180	arg1	fermentation					1091:1102	fermentation	1091:1102	fermentation	1091:1102	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	13	30	with	alterations	2156:2166	arg1	contribution					2264:2275	the potential contribution	2250:2275	the potential contribution to food digestion	2250:2293	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	3	31	theme	sea	619:621	arg1	urchins					623:629	Ulva-fed sea urchins	610:629	Ulva-fed sea urchins	610:629	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	15	32	theme	released	2625:2632	arg1	sulfates					2634:2641	the released sulfates	2621:2641	the released sulfates	2621:2641	It points to keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy.
37097162	15	33	theme	polysaccharides	2577:2591	arg1	digestion					2543:2551	the digestion	2539:2551	the digestion of the complex sulfated polysaccharides in dietary Ulva	2539:2607	It points to keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy.
37097162	5	34	theme	food	878:881	arg1	digestive					883:891	food digestive	878:891	food digestive	878:891	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	6	35	theme	Ulva	990:993	arg1	digestion					965:973	the digestion	961:973	the digestion of the dietary Ulva	961:993	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	14	36	from	distant	2434:2440	arg1	organ					2466:2470	this organ	2461:2470	this organ	2461:2470	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	5	37	from	digestive	883:891	arg1	community					835:843	The esophageal community	820:843	The esophageal community	820:843	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	5	37	from	digestive	883:891	arg1	diverse					854:860	diverse	854:860	diverse	854:860	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	5	37	from	digestive	883:891	arg1	poor					870:873	poor	870:873	poor	870:873	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	1	38	theme	algivorous	149:158	arg1	urchins					164:170	algivorous sea urchins	149:170	algivorous sea urchins such as Tripneustes gratilla elatensis	149:209	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	1	38	theme	algivorous	149:158	arg1	Tripneustes					180:190	Tripneustes	180:190	Tripneustes	180:190	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	10	39	theme	food	1828:1831	arg1	digestion					1833:1841	food digestion	1828:1841	food digestion	1828:1841	We conclude that the succession of microbial communities along the gut obtained supports the hypothesis on bacterial contribution to food digestion.
37097162	11	40	theme	new	1966:1968	arg1	reefs					1970:1974	constructing new reefs	1953:1974	constructing new reefs	1953:1974	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37097162	6	41	dep	stomach	1014:1020	arg1	the					1010:1012	the	1010:1012	the	1010:1012	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	7	42	from	digestion	1294:1302	arg1	necessary					1276:1284	necessary	1276:1284	necessary	1276:1284	Bacteroidetes and Firmicutes were found as the main phyla in the gut and are presumably also necessary in food digestion.
37097162	8	43	theme	abundant	1309:1316	arg1	bacteria					1335:1342	The abundant sulfate-reducing bacteria	1305:1342	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea	1305:1435	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	2	44	theme	host-microbe	397:408	arg1	relations					410:418	host-microbe relations	397:418	host-microbe relations	397:418	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	0	45	theme	Sea	98:100	arg1	Urchin					102:107	an Algivorous Sea Urchin	84:107	an Algivorous Sea Urchin	84:107	Spatial Succession Underlies Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin.
37097162	1	46	from	influence	118:126	arg1	microbiome					135:144	the microbiome	131:144	the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis	131:209	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	7	47	theme	food	1289:1292	arg1	digestion					1294:1302	food digestion	1289:1302	food digestion	1289:1302	Bacteroidetes and Firmicutes were found as the main phyla in the gut and are presumably also necessary in food digestion.
37097162	2	48	theme	metabolic	456:464	arg1	activity					496:503	activity	496:503	activity	496:503	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	2	48	theme	metabolic	456:464	arg1	repertoire					466:475	their metabolic repertoire	450:475	their metabolic repertoire	450:475	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	0	49	from	Contribution	39:50	arg1	Gut					77:79	the Gut	73:79	the Gut of an Algivorous Sea Urchin	73:107	Spatial Succession Underlies Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin.
37097162	0	50	theme	Spatial	0:6	arg1	Succession					8:17	Spatial Succession	0:17	Spatial Succession	0:17	Spatial Succession Underlies Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin.
37097162	1	51	theme	seaweed	276:282	arg1	digestion					252:260	the digestion	248:260	the digestion of fiber-rich seaweed	248:282	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	9	52	theme	sea	1544:1546	arg1	urchins					1548:1554	these sea urchins	1538:1554	these sea urchins	1538:1554	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	8	53	theme	algal	1506:1510	arg1	polysaccharides					1512:1526	the algal polysaccharides	1502:1526	the algal polysaccharides	1502:1526	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	2	54	theme	involved	435:442	arg1	taxa					444:447	the involved taxa	431:447	the involved taxa	431:447	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	13	55	theme	bacterial	2175:2183	arg1	structure					2199:2207	the bacterial compositional structure	2171:2207	the bacterial compositional structure along the gut	2171:2221	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	3	56	dep	esophagus	573:581	arg1	the					569:571	the	569:571	the	569:571	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	3	57	theme	rRNA	643:646	arg1	sequencing					657:666	16S rRNA amplicon sequencing	639:666	16S rRNA amplicon sequencing	639:666	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	1	58	theme	sea	160:162	arg1	urchins					164:170	algivorous sea urchins	149:170	algivorous sea urchins such as Tripneustes gratilla elatensis	149:209	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	1	58	theme	sea	160:162	arg1	Tripneustes					180:190	Tripneustes	180:190	Tripneustes	180:190	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	10	59	theme	bacterial	1802:1810	arg1	contribution					1812:1823	bacterial contribution	1802:1823	bacterial contribution to food digestion	1802:1841	We conclude that the succession of microbial communities along the gut obtained supports the hypothesis on bacterial contribution to food digestion.
37097162	2	60	theme	gut	343:345	arg1	community					357:365	the gut bacterial community	339:365	the gut bacterial community	339:365	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	0	61	theme	Food	55:58	arg1	Digestion					60:68	Food Digestion	55:68	Food Digestion	55:68	Spatial Succession Underlies Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin.
37097162	14	62	from	organ	2466:2470	arg1	distant					2434:2440	distant	2434:2440	distant	2434:2440	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	13	63	from	association	2233:2243	arg1	structure					2199:2207	the bacterial compositional structure	2171:2207	the bacterial compositional structure along the gut	2171:2221	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	11	64	dep	Tripneustes	1886:1896	arg1	elatensis					1907:1915	Tripneustes gratilla elatensis	1886:1915	the sea urchin Tripneustes gratilla elatensis	1871:1915	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37097162	1	65	dep	Tripneustes	180:190	arg1	elatensis					201:209	Tripneustes gratilla elatensis	180:209	Tripneustes gratilla elatensis	180:209	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	11	66	theme	IMPORTANCE	1844:1853	arg1	grazing					1860:1866	IMPORTANCE Alga grazing	1844:1866	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis	1844:1915	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37097162	14	67	theme	gut	2346:2348	arg1	ecology					2361:2367	the sea urchin's gut microbiome ecology	2329:2367	the sea urchin's gut microbiome ecology	2329:2367	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	9	68	theme	polysaccharides	1605:1619	arg1	degradation					1590:1600	the degradation	1586:1600	the degradation of polysaccharides of other algae and plants	1586:1645	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	2	69	theme	ecological	288:297	arg1	insight					299:305	An ecological insight	285:305	An ecological insight into the spatial arrangement in the gut bacterial community	285:365	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	16	70	from	diets	2748:2752	arg1	energy					2736:2741	energy	2736:2741	energy from diets of other algae and plants	2736:2778	The gut bacteria's genomic arsenal may also help to gain energy from diets of other algae and plants.
37097162	10	71	theme	communities	1740:1750	arg1	succession					1716:1725	the succession	1712:1725	the succession of microbial communities along the gut obtained	1712:1773	We conclude that the succession of microbial communities along the gut obtained supports the hypothesis on bacterial contribution to food digestion.
37097162	9	72	theme	other	1624:1628	arg1	algae					1630:1634	other algae	1624:1634	other algae	1624:1634	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	15	73	theme	sulfated	2568:2575	arg1	polysaccharides					2577:2591	the complex sulfated polysaccharides	2556:2591	the complex sulfated polysaccharides	2556:2591	It points to keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy.
37097162	4	74	dep	esophagus	795:803	arg1	the					791:793	the	791:793	the	791:793	We revealed communities with distinct features, especially those in the esophagus and intestine.
37097162	14	75	theme	bacterial	2403:2411	arg1	communities					2413:2423	distinct bacterial communities	2394:2423	distinct bacterial communities	2394:2423	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	3	76	theme	functional	705:714	arg1	genes					716:720	their functional genes	699:720	their functional genes	699:720	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	2	77	theme	spatial	316:322	arg1	arrangement					324:334	the spatial arrangement	312:334	the spatial arrangement in the gut bacterial community	312:365	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	14	78	from	other	2452:2456	arg1	distant					2434:2440	distant	2434:2440	distant	2434:2440	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	6	79	theme	acids	1136:1140	arg1	decomposition					1076:1088	carbohydrate decomposition	1063:1088	carbohydrate decomposition	1063:1088	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	79	theme	acids	1136:1140	arg1	ways					1155:1158	various ways	1147:1158	various ways of N and S metabolism	1147:1180	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	79	theme	acids	1136:1140	arg1	synthesis					1105:1113	synthesis	1105:1113	synthesis of short-chain fatty acids	1105:1140	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	79	theme	acids	1136:1140	arg1	fermentation					1091:1102	fermentation	1091:1102	fermentation	1091:1102	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	14	80	theme	spatial	2308:2314	arg1	insight					2316:2322	The current spatial insight	2296:2322	The current spatial insight into the sea urchin's gut microbiome ecology	2296:2367	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	14	80	theme	spatial	2308:2314	arg1	novel					2372:2376	novel	2372:2376	novel	2372:2376	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	16	81	theme	plants	2773:2778	arg1	diets					2748:2752	diets	2748:2752	diets of other algae and plants	2748:2778	The gut bacteria's genomic arsenal may also help to gain energy from diets of other algae and plants.
37097162	14	82	theme	current	2300:2306	arg1	insight					2316:2322	The current spatial insight	2296:2322	The current spatial insight into the sea urchin's gut microbiome ecology	2296:2367	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	14	82	theme	current	2300:2306	arg1	novel					2372:2376	novel	2372:2376	novel	2372:2376	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	5	83	theme	esophageal	824:833	arg1	community					835:843	The esophageal community	820:843	The esophageal community	820:843	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	5	83	theme	esophageal	824:833	arg1	diverse					854:860	diverse	854:860	diverse	854:860	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	5	83	theme	esophageal	824:833	arg1	poor					870:873	poor	870:873	poor	870:873	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	6	84	theme	short-chain	1118:1128	arg1	acids					1136:1140	short-chain fatty acids	1118:1140	short-chain fatty acids	1118:1140	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	9	85	theme	sea	1668:1670	arg1	microbiome					1683:1692	this sea urchin gut microbiome	1663:1692	this sea urchin gut microbiome	1663:1692	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	16	86	theme	gut	2683:2685	arg1	arsenal					2706:2712	The gut bacteria's genomic arsenal	2679:2712	The gut bacteria's genomic arsenal	2679:2712	The gut bacteria's genomic arsenal may also help to gain energy from diets of other algae and plants.
37097162	8	87	dep	stomach	1351:1357	arg1	the					1347:1349	the	1347:1349	the	1347:1349	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	4	88	with	communities	735:745	arg1	features					761:768	distinct features	752:768	distinct features	752:768	We revealed communities with distinct features, especially those in the esophagus and intestine.
37097162	4	88	with	communities	735:745	arg1	those					782:786	those	782:786	those	782:786	We revealed communities with distinct features, especially those in the esophagus and intestine.
37097162	5	89	theme	fermentation	896:907	arg1	genes					909:913	fermentation genes	896:913	fermentation genes	896:913	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	9	90	theme	gut	1679:1681	arg1	microbiome					1683:1692	this sea urchin gut microbiome	1663:1692	this sea urchin gut microbiome	1663:1692	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	3	91	from	communities	554:564	arg1	stomach					584:590	stomach	584:590	stomach	584:590	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	3	91	from	communities	554:564	arg1	intestine					597:605	intestine	597:605	intestine	597:605	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	3	91	from	communities	554:564	arg1	esophagus					573:581	esophagus	573:581	esophagus	573:581	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	3	92	theme	Ulva-fed	610:617	arg1	urchins					623:629	Ulva-fed sea urchins	610:629	Ulva-fed sea urchins	610:629	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	8	93	dep	genera	1382:1387	arg1	Desulfitispora					1403:1416	Desulfitispora	1403:1416	Desulfitispora	1403:1416	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	8	93	dep	genera	1382:1387	arg1	Defluviitalea					1423:1435	Defluviitalea	1423:1435	Defluviitalea	1423:1435	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	8	93	dep	genera	1382:1387	arg1	Desulfotalea					1389:1400	Desulfotalea	1389:1400	Desulfotalea	1389:1400	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	16	94	theme	algae	2763:2767	arg1	diets					2748:2752	diets	2748:2752	diets of other algae and plants	2748:2778	The gut bacteria's genomic arsenal may also help to gain energy from diets of other algae and plants.
37097162	9	95	from	microbiome	1683:1692	arg1	present					1652:1658	present	1652:1658	present	1652:1658	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	6	96	theme	S	1169:1169	arg1	metabolism					1171:1180	S metabolism	1169:1180	S metabolism	1169:1180	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	12	97	theme	bacteria	2041:2048	arg1	knowledge					2028:2036	the knowledge	2024:2036	the knowledge of bacteria that may aid this host in alga digestion and their phylogeny, roles, and activity niches	2024:2137	This research was driven by the need to expand the knowledge of bacteria that may aid this host in alga digestion and their phylogeny, roles, and activity niches.
37097162	5	98	from	poor	870:873	arg1	digestive					883:891	food digestive	878:891	food digestive	878:891	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	5	98	from	poor	870:873	arg1	genes					909:913	fermentation genes	896:913	fermentation genes	896:913	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	2	99	theme	activity	496:503	arg1	activity					496:503	activity	496:503	activity	496:503	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	2	99	theme	activity	496:503	arg1	taxa					444:447	the involved taxa	431:447	the involved taxa	431:447	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	2	99	theme	activity	496:503	arg1	niches					486:491	the niches	482:491	the niches of activity	482:503	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	2	99	theme	activity	496:503	arg1	repertoire					466:475	their metabolic repertoire	450:475	their metabolic repertoire	450:475	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	8	100	theme	excess	1461:1466	arg1	sulfate					1468:1474	the excess sulfate	1457:1474	the excess sulfate from the decomposition of the algal polysaccharides	1457:1526	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	6	101	theme	N	1163:1163	arg1	decomposition					1076:1088	carbohydrate decomposition	1063:1088	carbohydrate decomposition	1063:1088	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	101	theme	N	1163:1163	arg1	ways					1155:1158	various ways	1147:1158	various ways of N and S metabolism	1147:1180	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	101	theme	N	1163:1163	arg1	synthesis					1105:1113	synthesis	1105:1113	synthesis of short-chain fatty acids	1105:1140	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	101	theme	N	1163:1163	arg1	fermentation					1091:1102	fermentation	1091:1102	fermentation	1091:1102	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	13	102	from	alterations	2156:2166	arg1	structure					2199:2207	the bacterial compositional structure	2171:2207	the bacterial compositional structure along the gut	2171:2221	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	0	103	theme	Algivorous	87:96	arg1	Urchin					102:107	an Algivorous Sea Urchin	84:107	an Algivorous Sea Urchin	84:107	Spatial Succession Underlies Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin.
37097162	16	104	theme	other	2757:2761	arg1	algae					2763:2767	other algae	2757:2767	other algae	2757:2767	The gut bacteria's genomic arsenal may also help to gain energy from diets of other algae and plants.
37097162	9	105	from	present	1652:1658	arg1	microbiome					1683:1692	this sea urchin gut microbiome	1663:1692	this sea urchin gut microbiome	1663:1692	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	6	106	from	common	1000:1005	arg1	contrast					919:926	contrast	919:926	contrast	919:926	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	106	from	common	1000:1005	arg1	intestine					1026:1034	intestine	1026:1034	intestine	1026:1034	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	6	106	from	common	1000:1005	arg1	stomach					1014:1020	stomach	1014:1020	stomach	1014:1020	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	4	107	theme	distinct	752:759	arg1	features					761:768	distinct features	752:768	distinct features	752:768	We revealed communities with distinct features, especially those in the esophagus and intestine.
37097162	4	107	theme	distinct	752:759	arg1	those					782:786	those	782:786	those	782:786	We revealed communities with distinct features, especially those in the esophagus and intestine.
37097162	0	108	theme	Urchin	102:107	arg1	Gut					77:79	the Gut	73:79	the Gut of an Algivorous Sea Urchin	73:107	Spatial Succession Underlies Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin.
37097162	13	109	with	association	2233:2243	arg1	contribution					2264:2275	the potential contribution	2250:2275	the potential contribution to food digestion	2250:2293	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	1	110	from	microbiome	135:144	arg1	urchins					164:170	algivorous sea urchins	149:170	algivorous sea urchins such as Tripneustes gratilla elatensis	149:209	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	1	110	from	microbiome	135:144	arg1	Tripneustes					180:190	Tripneustes	180:190	Tripneustes	180:190	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	13	111	theme	food	2280:2283	arg1	digestion					2285:2293	food digestion	2280:2293	food digestion	2280:2293	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	15	112	from	digestion	2543:2551	arg1	Ulva					2604:2607	dietary Ulva	2596:2607	dietary Ulva	2596:2607	It points to keynote bacteria with genes that may aid the host in the digestion of the complex sulfated polysaccharides in dietary Ulva by removing the released sulfates and fermentation to provide energy.
37097162	11	113	theme	sea	1875:1877	arg1	urchin					1879:1884	the sea urchin Tripneustes gratilla elatensis	1871:1915	the sea urchin Tripneustes gratilla elatensis	1871:1915	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37097162	8	114	theme	polysaccharides	1512:1526	arg1	decomposition					1485:1497	the decomposition	1481:1497	the decomposition of the algal polysaccharides	1481:1526	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	8	115	theme	sulfate-reducing	1318:1333	arg1	bacteria					1335:1342	The abundant sulfate-reducing bacteria	1305:1342	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea	1305:1435	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	0	116	theme	Microbial	29:37	arg1	Contribution					39:50	Microbial Contribution	29:50	Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin	29:107	Spatial Succession Underlies Microbial Contribution to Food Digestion in the Gut of an Algivorous Sea Urchin.
37097162	5	117	from	genes	909:913	arg1	community					835:843	The esophageal community	820:843	The esophageal community	820:843	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	5	117	from	genes	909:913	arg1	diverse					854:860	diverse	854:860	diverse	854:860	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	5	117	from	genes	909:913	arg1	poor					870:873	poor	870:873	poor	870:873	The esophageal community was less diverse and was poor in food digestive or fermentation genes.
37097162	3	118	theme	16S	639:641	arg1	sequencing					657:666	16S rRNA amplicon sequencing	639:666	16S rRNA amplicon sequencing	639:666	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	13	119	theme	compositional	2185:2197	arg1	structure					2199:2207	the bacterial compositional structure	2171:2207	the bacterial compositional structure along the gut	2171:2221	We hypothesized alterations in the bacterial compositional structure along the gut and their association with the potential contribution to food digestion.
37097162	10	120	from	hypothesis	1788:1797	arg1	contribution					1812:1823	bacterial contribution	1802:1823	bacterial contribution to food digestion	1802:1841	We conclude that the succession of microbial communities along the gut obtained supports the hypothesis on bacterial contribution to food digestion.
37097162	3	121	theme	urchins	623:629	arg1	communities					554:564	the bacterial communities	540:564	the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins	540:629	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	2	122	theme	relations	410:418	arg1	knowledge					384:392	our knowledge	380:392	our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity	380:503	An ecological insight into the spatial arrangement in the gut bacterial community will improve our knowledge of host-microbe relations concerning the involved taxa, their metabolic repertoire, and the niches of activity.
37097162	8	123	from	decomposition	1485:1497	arg1	sulfate					1468:1474	the excess sulfate	1457:1474	the excess sulfate from the decomposition of the algal polysaccharides	1457:1526	The abundant sulfate-reducing bacteria in the stomach and intestine from the genera Desulfotalea, Desulfitispora, and Defluviitalea may aid in removing the excess sulfate from the decomposition of the algal polysaccharides.
37097162	6	124	from	contrast	919:926	arg1	common					1000:1005	common	1000:1005	common	1000:1005	In contrast, bacteria that can contribute to the digestion of the dietary Ulva were common in the stomach and intestine and consisted of genes for carbohydrate decomposition, fermentation, synthesis of short-chain fatty acids, and various ways of N and S metabolism.
37097162	12	125	theme	activity	2123:2130	arg1	niches					2132:2137	activity niches	2123:2137	activity niches	2123:2137	This research was driven by the need to expand the knowledge of bacteria that may aid this host in alga digestion and their phylogeny, roles, and activity niches.
37097162	9	126	attach	present	1652:1658	arg1	microbiome					1683:1692	this sea urchin gut microbiome	1663:1692	this sea urchin gut microbiome	1663:1692	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	9	126	attach	present	1652:1658	arg2	genes					1576:1580	genes	1576:1580	genes for the degradation of polysaccharides of other algae and plants	1576:1645	Although these sea urchins were fed with Ulva, genes for the degradation of polysaccharides of other algae and plants were present in this sea urchin gut microbiome.
37097162	11	127	dep	urchin	1879:1884	arg1	Tripneustes					1886:1896	Tripneustes	1886:1896	Tripneustes	1886:1896	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37097162	7	128	theme	main	1230:1233	arg1	phyla					1235:1239	the main phyla	1226:1239	the main phyla in the gut	1226:1250	Bacteroidetes and Firmicutes were found as the main phyla in the gut and are presumably also necessary in food digestion.
37097162	7	128	theme	main	1230:1233	arg1	Firmicutes					1201:1210	Firmicutes	1201:1210	Firmicutes	1201:1210	Bacteroidetes and Firmicutes were found as the main phyla in the gut and are presumably also necessary in food digestion.
37097162	7	128	theme	main	1230:1233	arg1	Bacteroidetes					1183:1195	Bacteroidetes	1183:1195	Bacteroidetes	1183:1195	Bacteroidetes and Firmicutes were found as the main phyla in the gut and are presumably also necessary in food digestion.
37097162	3	129	theme	amplicon	648:655	arg1	sequencing					657:666	16S rRNA amplicon sequencing	639:666	16S rRNA amplicon sequencing	639:666	Toward this goal, we investigated the bacterial communities in the esophagus, stomach, and intestine of Ulva-fed sea urchins through 16S rRNA amplicon sequencing, followed by the prediction of their functional genes.
37097162	1	130	theme	bacterial	222:230	arg1	contribution					232:243	a bacterial contribution	220:243	a bacterial contribution to the digestion of fiber-rich seaweed	220:282	Dietary influence on the microbiome in algivorous sea urchins such as Tripneustes gratilla elatensis suggests a bacterial contribution to the digestion of fiber-rich seaweed.
37097162	7	131	from	necessary	1276:1284	arg1	digestion					1294:1302	food digestion	1289:1302	food digestion	1289:1302	Bacteroidetes and Firmicutes were found as the main phyla in the gut and are presumably also necessary in food digestion.
37097162	14	132	theme	microbiome	2350:2359	arg1	ecology					2361:2367	the sea urchin's gut microbiome ecology	2329:2367	the sea urchin's gut microbiome ecology	2329:2367	The current spatial insight into the sea urchin's gut microbiome ecology is novel and reveals how distinct bacterial communities are when distant from each other in this organ.
37097162	11	133	theme	Alga	1855:1858	arg1	grazing					1860:1866	IMPORTANCE Alga grazing	1844:1866	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis	1844:1915	IMPORTANCE Alga grazing by the sea urchin Tripneustes gratilla elatensis is vital for nutrient recycling and constructing new reefs.
37201665	4	0	theme	amarus	552:557	arg1	parts					540:544	fresh aerial parts	527:544	fresh aerial parts of P. amarus	527:557	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	7	1	theme	extract	1136:1142	arg1	composition					1111:1121	the chemical composition	1098:1121	the chemical composition of the crude extract	1098:1142	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	9	2	theme	glucose	1438:1444	arg1	uptake					1446:1451	glucose uptake	1438:1451	glucose uptake similar to metformin	1438:1472	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	2	3	theme	several	282:288	arg1	countries					290:298	several countries	282:298	several countries	282:298	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus have been extensively used in several countries to cure diabetes.
37201665	9	4	theme	similar	1453:1459	arg1	uptake					1446:1451	glucose uptake	1438:1451	glucose uptake similar to metformin	1438:1472	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	6	5	theme	digestion	879:887	arg1	influence					840:848	The influence	836:848	The influence of in vitro gastrointestinal digestion	836:887	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	10	6	theme	enriched	1575:1582	arg1	fraction					1600:1607	the enriched polysaccharides fraction	1571:1607	the enriched polysaccharides fraction	1571:1607	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	7	7	theme	composition	1111:1121	arg1	Analysis					1086:1093	RESULTS Analysis	1078:1093	RESULTS Analysis of the chemical composition of the crude extract	1078:1142	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	7	8	theme	families	1197:1204	arg1	presence					1157:1164	the presence	1153:1164	the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans	1153:1275	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	5	9	theme	phase	817:821	arg1	UPLC-DAD-MS					823:833	reverse phase UPLC-DAD-MS	809:833	reverse phase UPLC-DAD-MS	809:833	MATERIALS AND METHODS An aqueous extract was obtained by an infusion method and its polyphenolic composition was analysed by reverse phase UPLC-DAD-MS.
37201665	4	10	theme	antidiabetic	616:627	arg1	effects					629:635	antidiabetic effects	616:635	antidiabetic effects occurring at the level of glucose homeostasis	616:681	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	3	11	from	impact	346:351	arg1	activity					428:435	their antidiabetic activity	409:435	their antidiabetic activity	409:435	No data is available on the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity.
37201665	9	12	theme	%	1506:1506	arg1	increase					1482:1489	an increase	1479:1489	an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively	1479:1537	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	4	13	theme	STUDY	449:453	arg1	AIM					438:440	AIM	438:440	AIM OF THE STUDY	438:453	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	1	14	dep	1827	61:64	arg1	activity					88:95	diabetes-related activity	71:95	diabetes-related activity	71:95	(1827) and diabetes-related activity of an aqueous extract as affected by in vitro gastrointestinal digestion.
37201665	10	15	theme	strong	1647:1652	arg1	activity					1673:1680	strong anti-hyperglycemic activity	1647:1680	strong anti-hyperglycemic activity	1647:1680	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	2	16	theme	aerial	218:223	arg1	part					225:228	the aerial part	214:228	the aerial part of Phyllanthus amarus	214:250	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus have been extensively used in several countries to cure diabetes.
37201665	1	17	theme	aqueous	103:109	arg1	extract					111:117	an aqueous extract	100:117	an aqueous extract	100:117	(1827) and diabetes-related activity of an aqueous extract as affected by in vitro gastrointestinal digestion.
37201665	6	18	theme	chemical	915:922	arg1	composition					924:934	the chemical composition	911:934	the chemical composition	911:934	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	13	19	theme	strong	1995:2000	arg1	inhibition					2024:2033	strong glucose-6-phosphatase inhibition	1995:2033	strong glucose-6-phosphatase inhibition	1995:2033	The dialyzed fraction showed strong glucose-6-phosphatase inhibition.
37201665	1	20	theme	extract	111:117	arg1	1827					61:64	1827	61:64	1827	61:64	(1827) and diabetes-related activity of an aqueous extract as affected by in vitro gastrointestinal digestion.
37201665	8	21	theme	polyphenols	1326:1336	arg1	content					1315:1321	the total content	1305:1321	the total content of polyphenols	1305:1336	After simulated digestion, the total content of polyphenols decreased by about 95%.
37201665	11	22	theme	lignan	1805:1810	arg1	isomers					1770:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	11	22	theme	lignan	1805:1810	arg1	demethyleneniranthin					1812:1831	lignan demethyleneniranthin	1805:1831	lignan demethyleneniranthin	1805:1831	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	2	23	theme	amarus	245:250	arg1	part					225:228	the aerial part	214:228	the aerial part of Phyllanthus amarus	214:250	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus have been extensively used in several countries to cure diabetes.
37201665	4	24	theme	homeostasis	671:681	arg1	level					654:658	the level	650:658	the level of glucose homeostasis	650:681	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	11	25	theme	first	1855:1859	arg1	time					1861:1864	the first time	1851:1864	the first time in the species	1851:1879	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	5	26	theme	infusion	744:751	arg1	method					753:758	an infusion method	741:758	an infusion method	741:758	MATERIALS AND METHODS An aqueous extract was obtained by an infusion method and its polyphenolic composition was analysed by reverse phase UPLC-DAD-MS.
37201665	6	27	theme	infusion	980:987	arg1	extract					989:995	P. amarus infusion extract	970:995	P. amarus infusion extract	970:995	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	5	28	theme	aqueous	709:715	arg1	extract					717:723	An aqueous extract	706:723	An aqueous extract	706:723	MATERIALS AND METHODS An aqueous extract was obtained by an infusion method and its polyphenolic composition was analysed by reverse phase UPLC-DAD-MS.
37201665	6	29	theme	P.	970:971	arg1	extract					989:995	P. amarus infusion extract	970:995	P. amarus infusion extract	970:995	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	7	30	theme	polyphenols	1209:1219	arg1	presence					1157:1164	the presence	1153:1164	the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans	1153:1275	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	6	31	theme	enzyme	1025:1030	arg1	inhibition					1032:1041	glucose-6-phosphatase enzyme inhibition	1003:1041	glucose-6-phosphatase enzyme inhibition	1003:1041	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	1	32	dep	in	134:135	arg1	vitro					137:141	vitro	137:141	vitro	137:141	(1827) and diabetes-related activity of an aqueous extract as affected by in vitro gastrointestinal digestion.
37201665	3	33	theme	antidiabetic	415:426	arg1	activity					428:435	their antidiabetic activity	409:435	their antidiabetic activity	409:435	No data is available on the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity.
37201665	0	34	theme	Polyphenolic	0:11	arg1	compounds					13:21	Polyphenolic compounds	0:21	Polyphenolic compounds of Phyllanthus amarus	0:43	Polyphenolic compounds of Phyllanthus amarus Schum & Thonn.
37201665	1	35	theme	diabetes-related	71:86	arg1	activity					88:95	diabetes-related activity	71:95	diabetes-related activity	71:95	(1827) and diabetes-related activity of an aqueous extract as affected by in vitro gastrointestinal digestion.
37201665	6	36	theme	uptake	1070:1075	arg1	stimulation					1047:1057	stimulation	1047:1057	stimulation of glucose uptake	1047:1075	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	6	36	theme	uptake	1070:1075	arg1	inhibition					1032:1041	glucose-6-phosphatase enzyme inhibition	1003:1041	glucose-6-phosphatase enzyme inhibition	1003:1041	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	3	37	theme	gastrointestinal	356:371	arg1	digestion					373:381	gastrointestinal digestion	356:381	gastrointestinal digestion of such crude extracts	356:404	No data is available on the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity.
37201665	4	38	theme	fresh	527:531	arg1	parts					540:544	fresh aerial parts	527:544	fresh aerial parts of P. amarus	527:557	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	6	39	theme	in	853:854	arg1	digestion					879:887	in vitro gastrointestinal digestion	853:887	in vitro gastrointestinal digestion	853:887	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	4	40	theme	parts	540:544	arg1	compounds					514:522	compounds	514:522	compounds of fresh aerial parts of P. amarus	514:557	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	10	41	theme	inhibition	1725:1734	arg1	%					1698:1698	about 39-62%	1687:1698	about 39-62% of glucose-6-phosphatase inhibition	1687:1734	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	10	41	theme	inhibition	1725:1734	arg1	inhibition					1725:1734	glucose-6-phosphatase inhibition	1703:1734	glucose-6-phosphatase inhibition	1703:1734	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	0	42	theme	amarus	38:43	arg1	compounds					13:21	Polyphenolic compounds	0:21	Polyphenolic compounds of Phyllanthus amarus	0:43	Polyphenolic compounds of Phyllanthus amarus Schum & Thonn.
37201665	9	43	theme	Caffeoylglucaric	1362:1377	arg1	derivates					1384:1392	Caffeoylglucaric acid derivates	1362:1392	Caffeoylglucaric acid derivates	1362:1392	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	12	44	theme	extract	1949:1955	arg1	composition					1930:1940	the composition	1926:1940	the composition of the extract	1926:1955	After in vitro gastroinstestinal digestion, the composition of the extract changed.
37201665	12	45	theme	in	1888:1889	arg1	digestion					1915:1923	in vitro gastroinstestinal digestion	1888:1923	in vitro gastroinstestinal digestion	1888:1923	After in vitro gastroinstestinal digestion, the composition of the extract changed.
37201665	3	46	theme	crude	391:395	arg1	extracts					397:404	such crude extracts	386:404	such crude extracts	386:404	No data is available on the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity.
37201665	11	47	theme	acid	1765:1768	arg1	isomers					1770:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	11	47	theme	acid	1765:1768	arg1	M1					1798:1799	tannin acalyphidin M1	1779:1799	tannin acalyphidin M1	1779:1799	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	11	47	theme	acid	1765:1768	arg1	demethyleneniranthin					1812:1831	lignan demethyleneniranthin	1805:1831	lignan demethyleneniranthin	1805:1831	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	5	48	theme	polyphenolic	768:779	arg1	composition					781:791	its polyphenolic composition	764:791	its polyphenolic composition	764:791	MATERIALS AND METHODS An aqueous extract was obtained by an infusion method and its polyphenolic composition was analysed by reverse phase UPLC-DAD-MS.
37201665	12	49	theme	gastroinstestinal	1897:1913	arg1	digestion					1915:1923	in vitro gastroinstestinal digestion	1888:1923	in vitro gastroinstestinal digestion	1888:1923	After in vitro gastroinstestinal digestion, the composition of the extract changed.
37201665	2	50	theme	RELEVANCE	192:200	arg1	Extracts					202:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts	171:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus	171:250	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus have been extensively used in several countries to cure diabetes.
37201665	9	51	theme	strong	1416:1421	arg1	stimulation					1423:1433	strong stimulation	1416:1433	strong stimulation of glucose uptake similar to metformin	1416:1472	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	2	52	used	used	274:277	arg2	Extracts					202:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts	171:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus	171:250	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus have been extensively used in several countries to cure diabetes.
37201665	7	53	theme	crude	1130:1134	arg1	extract					1136:1142	the crude extract	1126:1142	the crude extract	1126:1142	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	9	54	theme	uptake	1446:1451	arg1	stimulation					1423:1433	strong stimulation	1416:1433	strong stimulation of glucose uptake similar to metformin	1416:1472	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	10	55	theme	polysaccharides	1584:1598	arg1	fraction					1600:1607	the enriched polysaccharides fraction	1571:1607	the enriched polysaccharides fraction	1571:1607	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	7	56	theme	chemical	1102:1109	arg1	composition					1111:1121	the chemical composition	1098:1121	the chemical composition of the crude extract	1098:1142	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	1	57	theme	gastrointestinal	143:158	arg1	digestion					160:168	in vitro gastrointestinal digestion	134:168	in vitro gastrointestinal digestion	134:168	(1827) and diabetes-related activity of an aqueous extract as affected by in vitro gastrointestinal digestion.
37201665	6	58	theme	gastrointestinal	862:877	arg1	digestion					879:887	in vitro gastrointestinal digestion	853:887	in vitro gastrointestinal digestion	853:887	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	10	59	theme	bioaccessible	1617:1629	arg1	fraction					1631:1638	the bioaccessible fraction	1613:1638	the bioaccessible fraction	1613:1638	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	7	60	theme	RESULTS	1078:1084	arg1	Analysis					1086:1093	RESULTS Analysis	1078:1093	RESULTS Analysis of the chemical composition of the crude extract	1078:1142	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	5	61	theme	reverse	809:815	arg1	UPLC-DAD-MS					823:833	reverse phase UPLC-DAD-MS	809:833	reverse phase UPLC-DAD-MS	809:833	MATERIALS AND METHODS An aqueous extract was obtained by an infusion method and its polyphenolic composition was analysed by reverse phase UPLC-DAD-MS.
37201665	6	62	theme	antidiabetic	947:958	arg1	effect					960:965	the antidiabetic effect	943:965	the antidiabetic effect of P. amarus infusion extract	943:995	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	11	63	from	time	1861:1864	arg1	species					1873:1879	the species	1869:1879	the species	1869:1879	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	8	64	theme	simulated	1284:1292	arg1	digestion					1294:1302	simulated digestion	1284:1302	simulated digestion	1284:1302	After simulated digestion, the total content of polyphenols decreased by about 95%.
37201665	10	65	theme	anti-hyperglycemic	1654:1671	arg1	activity					1673:1680	strong anti-hyperglycemic activity	1647:1680	strong anti-hyperglycemic activity	1647:1680	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	2	66	theme	part	225:228	arg1	Extracts					202:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts	171:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus	171:250	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus have been extensively used in several countries to cure diabetes.
37201665	9	67	theme	%	1524:1524	arg1	increase					1482:1489	an increase	1479:1489	an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively	1479:1537	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	8	68	theme	total	1309:1313	arg1	content					1315:1321	the total content	1305:1321	the total content of polyphenols	1305:1336	After simulated digestion, the total content of polyphenols decreased by about 95%.
37201665	13	69	theme	glucose-6-phosphatase	2002:2022	arg1	inhibition					2024:2033	strong glucose-6-phosphatase inhibition	1995:2033	strong glucose-6-phosphatase inhibition	1995:2033	The dialyzed fraction showed strong glucose-6-phosphatase inhibition.
37201665	6	70	theme	glucose-6-phosphatase	1003:1023	arg1	inhibition					1032:1041	glucose-6-phosphatase enzyme inhibition	1003:1041	glucose-6-phosphatase enzyme inhibition	1003:1041	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	7	71	theme	phenolic	1229:1236	arg1	acids					1238:1242	phenolic acids	1229:1242	phenolic acids	1229:1242	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	1	72	theme	in	134:135	arg1	digestion					160:168	in vitro gastrointestinal digestion	134:168	in vitro gastrointestinal digestion	134:168	(1827) and diabetes-related activity of an aqueous extract as affected by in vitro gastrointestinal digestion.
37201665	6	73	theme	extract	989:995	arg1	effect					960:965	the antidiabetic effect	943:965	the antidiabetic effect of P. amarus infusion extract	943:995	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	5	74	dep	MATERIALS	684:692	arg1	obtained					729:736	obtained	729:736	was obtained by an infusion method and its polyphenolic composition	725:791	MATERIALS AND METHODS An aqueous extract was obtained by an infusion method and its polyphenolic composition was analysed by reverse phase UPLC-DAD-MS.
37201665	4	75	theme	active	493:498	arg1	fractions					500:508	active fractions	493:508	active fractions	493:508	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	4	76	theme	glucose	663:669	arg1	homeostasis					671:681	glucose homeostasis	663:681	glucose homeostasis	663:681	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	13	77	theme	dialyzed	1970:1977	arg1	fraction					1979:1986	The dialyzed fraction	1966:1986	The dialyzed fraction	1966:1986	The dialyzed fraction showed strong glucose-6-phosphatase inhibition.
37201665	6	78	theme	amarus	973:978	arg1	extract					989:995	P. amarus infusion extract	970:995	P. amarus infusion extract	970:995	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	4	79	theme	study	471:475	arg1	aim					459:461	The aim	455:461	The aim of this study	455:475	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	7	80	theme	various	1189:1195	arg1	families					1197:1204	various families	1189:1204	various families	1189:1204	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	3	81	theme	such	386:389	arg1	extracts					397:404	such crude extracts	386:404	such crude extracts	386:404	No data is available on the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity.
37201665	4	82	theme	aerial	533:538	arg1	parts					540:544	fresh aerial parts	527:544	fresh aerial parts of P. amarus	527:557	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	11	83	theme	acalyphidin	1786:1796	arg1	isomers					1770:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	11	83	theme	acalyphidin	1786:1796	arg1	M1					1798:1799	tannin acalyphidin M1	1779:1799	tannin acalyphidin M1	1779:1799	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	7	84	theme	polysaccharides	1169:1183	arg1	presence					1157:1164	the presence	1153:1164	the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans	1153:1275	RESULTS Analysis of the chemical composition of the crude extract revealed the presence of polysaccharides and various families of polyphenols such as phenolic acids, tannins, flavonoids and lignans.
37201665	6	85	theme	glucose	1062:1068	arg1	uptake					1070:1075	glucose uptake	1062:1075	glucose uptake	1062:1075	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	3	86	theme	digestion	373:381	arg1	impact					346:351	the impact	342:351	the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity	342:435	No data is available on the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity.
37201665	2	87	theme	ETHNOPHARMACOLOGICAL	171:190	arg1	Extracts					202:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts	171:209	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus	171:250	ETHNOPHARMACOLOGICAL RELEVANCE Extracts of the aerial part of Phyllanthus amarus have been extensively used in several countries to cure diabetes.
37201665	12	88	dep	in	1888:1889	arg1	vitro					1891:1895	vitro	1891:1895	vitro	1891:1895	After in vitro gastroinstestinal digestion, the composition of the extract changed.
37201665	0	89	dep	Thonn	53:57	arg1	compounds					13:21	Polyphenolic compounds	0:21	Polyphenolic compounds of Phyllanthus amarus	0:43	Polyphenolic compounds of Phyllanthus amarus Schum & Thonn.
37201665	11	90	theme	Caffeoylglucaric	1748:1763	arg1	isomers					1770:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	11	90	theme	Caffeoylglucaric	1748:1763	arg1	M1					1798:1799	tannin acalyphidin M1	1779:1799	tannin acalyphidin M1	1779:1799	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	11	90	theme	Caffeoylglucaric	1748:1763	arg1	demethyleneniranthin					1812:1831	lignan demethyleneniranthin	1805:1831	lignan demethyleneniranthin	1805:1831	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	10	91	theme	glucose-6-phosphatase	1703:1723	arg1	inhibition					1725:1734	glucose-6-phosphatase inhibition	1703:1734	glucose-6-phosphatase inhibition	1703:1734	Moreover, corilagin, geraniin, the enriched polysaccharides fraction and the bioaccessible fraction showed strong anti-hyperglycemic activity with about 39-62% of glucose-6-phosphatase inhibition.
37201665	9	92	theme	acid	1379:1382	arg1	derivates					1384:1392	Caffeoylglucaric acid derivates	1362:1392	Caffeoylglucaric acid derivates	1362:1392	Caffeoylglucaric acid derivates and lignans exhibited strong stimulation of glucose uptake similar to metformin with an increase of 35.62 ± 6.14% and 34.74 ± 5.33% respectively.
37201665	3	93	theme	extracts	397:404	arg1	digestion					373:381	gastrointestinal digestion	356:381	gastrointestinal digestion of such crude extracts	356:404	No data is available on the impact of gastrointestinal digestion of such crude extracts on their antidiabetic activity.
37201665	6	94	dep	in	853:854	arg1	vitro					856:860	vitro	856:860	vitro	856:860	The influence of in vitro gastrointestinal digestion was evaluated both on the chemical composition and on the antidiabetic effect of P. amarus infusion extract using glucose-6-phosphatase enzyme inhibition and stimulation of glucose uptake.
37201665	4	95	dep	was	477:479	arg1	AIM					438:440	AIM	438:440	AIM OF THE STUDY	438:453	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	4	96	theme	infusion	575:582	arg1	method					584:589	an infusion method	572:589	an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis	572:681	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	4	96	theme	infusion	575:582	arg1	responsible					600:610	responsible	600:610	responsible	600:610	AIM OF THE STUDY The aim of this study was to identify active fractions and compounds of fresh aerial parts of P. amarus extracted by an infusion method that are responsible for antidiabetic effects occurring at the level of glucose homeostasis.
37201665	11	97	theme	tannin	1779:1784	arg1	isomers					1770:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers	1737:1776	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
37201665	11	97	theme	tannin	1779:1784	arg1	M1					1798:1799	tannin acalyphidin M1	1779:1799	tannin acalyphidin M1	1779:1799	CONCLUSION Caffeoylglucaric acid isomers, tannin acalyphidin M1 and lignan demethyleneniranthin were reported for the first time in the species.
36119371	2	0	theme	gut	524:526	arg1	composition					539:549	the gut microbiota composition	520:549	the gut microbiota composition of diabetic mice	520:566	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	3	1	theme	B	821:821	arg1	expression					751:760	the mRNA expression	742:760	the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice	742:848	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	4	2	theme	signaling	1230:1238	arg1	pathways					1240:1247	PI3K/Akt-mediated signaling pathways	1212:1247	PI3K/Akt-mediated signaling pathways in T2DM mice	1212:1260	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	3	3	theme	beneficial	885:894	arg1	Lactobacillus					906:918	Lactobacillus	906:918	Lactobacillus	906:918	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	3	3	theme	beneficial	885:894	arg1	bacteria					896:903	beneficial bacteria	885:903	beneficial bacteria (Lactobacillus)	885:919	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	3	4	from	expression	751:760	arg1	liver					836:840	the liver	832:840	the liver of mice	832:848	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	0	5	from	potential	13:21	arg1	schreberi					56:64	schreberi	56:64	schreberi	56:64	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	3	6	theme	muscle	712:717	arg1	glycogen					719:726	muscle glycogen	712:726	muscle glycogen	712:726	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	3	7	theme	bacteria	896:903	arg1	abundance					872:880	the abundance	868:880	the abundance of beneficial bacteria (Lactobacillus)	868:919	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	3	8	theme	phosphatidylinositol-3-kinase	765:793	arg1	expression					751:760	the mRNA expression	742:760	the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice	742:848	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	0	9	theme	gut	107:109	arg1	microbiota					111:120	gut microbiota	107:120	gut microbiota	107:120	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	3	10	theme	protein	806:812	arg1	Akt					824:826	Akt	824:826	Akt	824:826	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	3	10	theme	protein	806:812	arg1	B					821:821	protein kinase B	806:821	protein kinase B (Akt)	806:827	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	4	11	theme	Lactobacillus	1186:1198	arg1	abundance					1173:1181	the abundance	1169:1181	the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice	1169:1260	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	2	12	theme	diabetic	554:561	arg1	mice					563:566	diabetic mice	554:566	diabetic mice	554:566	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	0	13	from	schreberi	56:64	arg1	polysaccharides					26:40	polysaccharides	26:40	polysaccharides from Brasenia schreberi	26:64	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	0	13	from	schreberi	56:64	arg1	potential					13:21	Antidiabetic potential	0:21	Antidiabetic potential of polysaccharides from Brasenia schreberi	0:64	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	2	14	theme	metabolic	458:466	arg1	inflammation					488:499	metabolic endotoxemia-related inflammation	458:499	metabolic endotoxemia-related inflammation	458:499	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	2	15	theme	endotoxemia-related	468:486	arg1	inflammation					488:499	metabolic endotoxemia-related inflammation	458:499	metabolic endotoxemia-related inflammation	458:499	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	3	16	theme	kinase	814:819	arg1	Akt					824:826	Akt	824:826	Akt	824:826	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	3	16	theme	kinase	814:819	arg1	B					821:821	protein kinase B	806:821	protein kinase B (Akt)	806:827	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	1	17	theme	hypoglycemic	183:194	arg1	activities					196:205	hypoglycemic activities	183:205	hypoglycemic activities	183:205	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	0	18	theme	Antidiabetic	0:11	arg1	potential					13:21	Antidiabetic potential	0:21	Antidiabetic potential of polysaccharides from Brasenia schreberi	0:64	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	4	19	theme	gut	1062:1064	arg1	dysbiosis					1077:1085	gut microbiota dysbiosis	1062:1085	gut microbiota dysbiosis	1062:1085	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	0	20	theme	polysaccharides	26:40	arg1	potential					13:21	Antidiabetic potential	0:21	Antidiabetic potential of polysaccharides from Brasenia schreberi	0:64	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	3	21	theme	mice	845:848	arg1	liver					836:840	the liver	832:840	the liver of mice	832:848	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	0	22	theme	diabetic	132:139	arg1	mice					141:144	type 2 diabetic mice	125:144	type 2 diabetic mice	125:144	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	2	23	theme	hyperglycemia	431:443	arg1	symptoms					419:426	the symptoms	415:426	the symptoms of hyperglycemia	415:443	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	1	24	theme	diabetic	295:302	arg1	mice					304:307	diabetic mice	295:307	diabetic mice induced by high-fat diet and streptozotocin	295:351	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	2	25	theme	Firmicutes/Bacteroidetes	597:620	arg1	ratios					622:627	significantly decreased Firmicutes/Bacteroidetes ratios	573:627	significantly decreased Firmicutes/Bacteroidetes ratios	573:627	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	1	26	theme	gut	211:213	arg1	effects					236:242	gut microbial regulation effects	211:242	gut microbial regulation effects	211:242	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	2	27	theme	decreased	587:595	arg1	ratios					622:627	significantly decreased Firmicutes/Bacteroidetes ratios	573:627	significantly decreased Firmicutes/Bacteroidetes ratios	573:627	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	0	28	from	pathway	95:101	arg1	mice					141:144	type 2 diabetic mice	125:144	type 2 diabetic mice	125:144	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	3	29	theme	microbiota	660:669	arg1	components					671:680	altered gut microbiota components	648:680	altered gut microbiota components	648:680	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	1	30	theme	microbial	215:223	arg1	effects					236:242	gut microbial regulation effects	211:242	gut microbial regulation effects	211:242	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	4	31	theme	probiotic	1032:1040	arg1	agent					1042:1046	a beneficial probiotic agent	1019:1046	a beneficial probiotic agent that reverses gut microbiota dysbiosis	1019:1085	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	31	theme	probiotic	1032:1040	arg1	polysaccharides					988:1002	BS polysaccharides	985:1002	BS polysaccharides	985:1002	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	1	32	from	schreberi	277:285	arg1	polysaccharides					247:261	polysaccharides	247:261	polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin	247:351	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	1	32	from	schreberi	277:285	arg1	effects					236:242	gut microbial regulation effects	211:242	gut microbial regulation effects	211:242	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	1	32	from	schreberi	277:285	arg1	activities					196:205	hypoglycemic activities	183:205	hypoglycemic activities	183:205	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	4	33	used	used	1011:1014	arg2	agent					1042:1046	a beneficial probiotic agent	1019:1046	a beneficial probiotic agent that reverses gut microbiota dysbiosis	1019:1085	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	33	used	used	1011:1014	arg2	polysaccharides					988:1002	BS polysaccharides	985:1002	BS polysaccharides	985:1002	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	1	34	dep	activities	196:205	arg1	the					179:181	the	179:181	the	179:181	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	4	35	theme	T2DM	1252:1255	arg1	mice					1257:1260	T2DM mice	1252:1260	T2DM mice	1252:1260	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	1	36	theme	regulation	225:234	arg1	effects					236:242	gut microbial regulation effects	211:242	gut microbial regulation effects	211:242	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	2	37	theme	microbiota	528:537	arg1	composition					539:549	the gut microbiota composition	520:549	the gut microbiota composition of diabetic mice	520:566	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	3	38	theme	altered	648:654	arg1	components					671:680	altered gut microbiota components	648:680	altered gut microbiota components	648:680	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	4	39	theme	first	956:960	arg1	time					962:965	the first time	952:965	the first time	952:965	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	1	40	theme	high-fat	320:327	arg1	diet					329:332	high-fat diet	320:332	high-fat diet	320:332	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	3	41	theme	mRNA	746:749	arg1	expression					751:760	the mRNA expression	742:760	the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice	742:848	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	3	42	theme	gut	656:658	arg1	components					671:680	altered gut microbiota components	648:680	altered gut microbiota components	648:680	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	4	43	theme	polysaccharides	1125:1139	arg1	mechanisms					1108:1117	the hypoglycemic mechanisms	1091:1117	the hypoglycemic mechanisms of BS polysaccharides	1091:1139	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	43	theme	polysaccharides	1125:1139	arg1	related					1148:1154	related	1148:1154	related	1148:1154	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	1	44	from	effects	236:242	arg1	schreberi					277:285	schreberi	277:285	schreberi	277:285	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	1	44	from	effects	236:242	arg1	BS					288:289	BS	288:289	BS	288:289	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	4	45	from	pathways	1240:1247	arg1	mice					1257:1260	T2DM mice	1252:1260	T2DM mice	1252:1260	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	3	46	theme	liver	693:697	arg1	glycogen					699:706	liver glycogen	693:706	liver glycogen	693:706	More importantly, altered gut microbiota components may affect liver glycogen and muscle glycogen by increasing the mRNA expression of phosphatidylinositol-3-kinase (PI3K) and protein kinase B (Akt) in the liver of mice through modulated the abundance of beneficial bacteria (Lactobacillus).
36119371	4	47	theme	BS	1122:1123	arg1	polysaccharides					1125:1139	BS polysaccharides	1122:1139	BS polysaccharides	1122:1139	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	0	48	from	microbiota	111:120	arg1	mice					141:144	type 2 diabetic mice	125:144	type 2 diabetic mice	125:144	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	4	49	theme	PI3K/Akt-mediated	1212:1228	arg1	pathways					1240:1247	PI3K/Akt-mediated signaling pathways	1212:1247	PI3K/Akt-mediated signaling pathways in T2DM mice	1212:1260	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	50	theme	BS	985:986	arg1	polysaccharides					988:1002	BS polysaccharides	985:1002	BS polysaccharides	985:1002	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	50	theme	BS	985:986	arg1	agent					1042:1046	a beneficial probiotic agent	1019:1046	a beneficial probiotic agent that reverses gut microbiota dysbiosis	1019:1085	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	0	51	theme	signaling	85:93	arg1	pathway					95:101	insulin signaling pathway	77:101	insulin signaling pathway	77:101	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	1	52	from	activities	196:205	arg1	schreberi					277:285	schreberi	277:285	schreberi	277:285	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	1	52	from	activities	196:205	arg1	BS					288:289	BS	288:289	BS	288:289	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	2	53	theme	BS	378:379	arg1	polysaccharides					381:395	BS polysaccharides	378:395	BS polysaccharides	378:395	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
36119371	1	54	theme	polysaccharides	247:261	arg1	effects					236:242	gut microbial regulation effects	211:242	gut microbial regulation effects	211:242	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	1	54	theme	polysaccharides	247:261	arg1	activities					196:205	hypoglycemic activities	183:205	hypoglycemic activities	183:205	This study aimed to investigate the hypoglycemic activities and gut microbial regulation effects of polysaccharides from Brasenia schreberi (BS) in diabetic mice induced by high-fat diet and streptozotocin.
36119371	0	55	theme	insulin	77:83	arg1	pathway					95:101	insulin signaling pathway	77:101	insulin signaling pathway	77:101	Antidiabetic potential of polysaccharides from Brasenia schreberi regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice.
36119371	4	56	theme	beneficial	1021:1030	arg1	agent					1042:1046	a beneficial probiotic agent	1019:1046	a beneficial probiotic agent that reverses gut microbiota dysbiosis	1019:1085	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	56	theme	beneficial	1021:1030	arg1	polysaccharides					988:1002	BS polysaccharides	985:1002	BS polysaccharides	985:1002	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	57	theme	microbiota	1066:1075	arg1	dysbiosis					1077:1085	gut microbiota dysbiosis	1062:1085	gut microbiota dysbiosis	1062:1085	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	58	theme	hypoglycemic	1095:1106	arg1	mechanisms					1108:1117	the hypoglycemic mechanisms	1091:1117	the hypoglycemic mechanisms of BS polysaccharides	1091:1139	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	4	58	theme	hypoglycemic	1095:1106	arg1	related					1148:1154	related	1148:1154	related	1148:1154	Altogether, our findings, for the first time, demonstrate that BS polysaccharides may be used as a beneficial probiotic agent that reverses gut microbiota dysbiosis and the hypoglycemic mechanisms of BS polysaccharides may be related to enhancing the abundance of Lactobacillus to activate PI3K/Akt-mediated signaling pathways in T2DM mice.
36119371	2	59	theme	mice	563:566	arg1	composition					539:549	the gut microbiota composition	520:549	the gut microbiota composition of diabetic mice	520:566	Our data indicated that BS polysaccharides not only improved the symptoms of hyperglycemia and relieved metabolic endotoxemia-related inflammation but also optimized the gut microbiota composition of diabetic mice with significantly decreased Firmicutes/Bacteroidetes ratios.
37298396	9	0	theme	significant	1734:1744	arg1	relationships					1755:1767	significant negative relationships	1734:1767	significant negative relationships	1734:1767	Desulfovibrio had significant negative relationships with alanine aminotransferase (ALT) and LPS levels.
37298396	8	1	theme	protein	1696:1702	arg1	expression					1704:1713	pathway protein expression	1688:1713	pathway protein expression	1688:1713	Spearman correlation analysis indicated that Lactobacillus and Desulfovibrio were positively correlated with ZO-1 or occludin protein expression and negatively correlated with pathway protein expression.
37298396	3	2	theme	C57BL/6J	458:465	arg1	mice					467:470	C57BL/6J mice	458:470	C57BL/6J mice	458:470	C57BL/6J mice were given three levels of Lc intragastrically for 2 h before administering isoniazid and rifampicin for 8 weeks.
37298396	2	3	theme	Lactobacillus	274:286	arg1	Lc					295:296	Lc	295:296	Lc	295:296	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	2	3	theme	Lactobacillus	274:286	arg1	casei					288:292	Lactobacillus casei	274:292	Lactobacillus casei (Lc)	274:297	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	5	4	theme	hepatocyte	1031:1040	arg1	necrosis					1042:1049	reduced hepatocyte necrosis	1023:1049	reduced hepatocyte necrosis	1023:1049	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	4	5	theme	polymerase	755:764	arg1	qRT-PCR					782:788	qRT-PCR	782:788	qRT-PCR	782:788	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	4	5	theme	polymerase	755:764	arg1	reaction					772:779	quantitative real time polymerase chain reaction	732:779	quantitative real time polymerase chain reaction (qRT-PCR)	732:789	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	9	6	theme	alanine	1774:1780	arg1	aminotransferase					1782:1797	alanine aminotransferase	1774:1797	alanine aminotransferase (ALT)	1774:1803	Desulfovibrio had significant negative relationships with alanine aminotransferase (ALT) and LPS levels.
37298396	9	6	theme	alanine	1774:1780	arg1	ALT					1800:1802	ALT	1800:1802	ALT	1800:1802	Desulfovibrio had significant negative relationships with alanine aminotransferase (ALT) and LPS levels.
37298396	8	7	theme	correlation	1521:1531	arg1	analysis					1533:1540	Spearman correlation analysis	1512:1540	Spearman correlation analysis	1512:1540	Spearman correlation analysis indicated that Lactobacillus and Desulfovibrio were positively correlated with ZO-1 or occludin protein expression and negatively correlated with pathway protein expression.
37298396	10	8	theme	pathway	1964:1970	arg1	proteins					1972:1979	pathway proteins	1964:1979	pathway proteins	1964:1979	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	4	9	theme	colon	604:608	arg1	tissues					610:616	colon tissues	604:616	colon tissues	604:616	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	6	10	theme	Lactobacillus	1158:1170	arg1	abundance					1145:1153	the abundance	1141:1153	the abundance of Lactobacillus and Desulfovibrio	1141:1188	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	1	11	theme	drug-induced	205:216	arg1	ATDILI					232:237	ATDILI	232:237	ATDILI	232:237	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	1	11	theme	drug-induced	205:216	arg1	injury					224:229	anti-tuberculosis drug-induced liver injury	187:229	anti-tuberculosis drug-induced liver injury (ATDILI)	187:238	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	7	12	theme	protein	1444:1450	arg1	expression					1452:1461	MyD88 protein expression	1438:1461	MyD88 protein expression (p < 0.05)	1438:1472	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	7	12	theme	protein	1444:1450	arg1	<					1466:1466	p < 0.05	1464:1471	p < 0.05	1464:1471	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	9	13	theme	LPS	1809:1811	arg1	levels					1813:1818	LPS levels	1809:1818	LPS levels	1809:1818	Desulfovibrio had significant negative relationships with alanine aminotransferase (ALT) and LPS levels.
37298396	10	14	theme	negative	1848:1855	arg1	associations					1857:1868	negative associations	1848:1868	negative associations with ZO-1, occludin, and claudin-1 protein expressions	1848:1923	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	4	15	theme	16S	796:798	arg1	analyses					805:812	16S rRNA analyses	796:812	16S rRNA analyses	796:812	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	4	16	theme	cecal	630:634	arg1	contents					636:643	cecal contents	630:643	cecal contents	630:643	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	10	17	with	correlations	1938:1949	arg1	ZO-1					1875:1878	ZO-1	1875:1878	ZO-1	1875:1878	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	17	with	correlations	1938:1949	arg1	LPS					1956:1958	LPS	1956:1958	LPS	1956:1958	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	17	with	correlations	1938:1949	arg1	proteins					1972:1979	pathway proteins	1964:1979	pathway proteins	1964:1979	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	17	with	correlations	1938:1949	arg1	occludin					1881:1888	occludin	1881:1888	occludin	1881:1888	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	17	with	correlations	1938:1949	arg1	claudin-1					1895:1903	claudin-1	1895:1903	claudin-1	1895:1903	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	5	18	theme	Lc	815:816	arg1	intervention					818:829	Lc intervention	815:829	Lc intervention	815:829	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	1	19	theme	gut-liver	129:137	arg1	axis					139:142	The gut-liver axis	125:142	The gut-liver axis	125:142	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	10	20	dep	ZO-1	1875:1878	arg1	expressions					1913:1923	protein expressions	1905:1923	protein expressions	1905:1923	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	2	21	theme	MyD88	442:446	arg1	pathway					449:455	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	8	22	theme	protein	1638:1644	arg1	expression					1646:1655	protein expression	1638:1655	protein expression	1638:1655	Spearman correlation analysis indicated that Lactobacillus and Desulfovibrio were positively correlated with ZO-1 or occludin protein expression and negatively correlated with pathway protein expression.
37298396	7	23	theme	lipopolysaccharide	1379:1396	arg1	level					1404:1408	the lipopolysaccharide (LPS) level	1375:1408	the lipopolysaccharide (LPS) level	1375:1408	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	12	24	theme	TLR4-NF-κB-MyD88	2157:2172	arg1	activation					2182:2191	TLR4-NF-κB-MyD88 pathway activation	2157:2191	TLR4-NF-κB-MyD88 pathway activation	2157:2191	Moreover, Lactobacillus casei may also inhibit TLR4-NF-κB-MyD88 pathway activation and alleviate ATDILI.
37298396	4	25	theme	histological	681:692	arg1	examination					694:704	biochemical and histological examination	665:704	biochemical and histological examination	665:704	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	5	26	theme	anti-tuberculosis	1088:1104	arg1	drugs					1106:1110	anti-tuberculosis drugs	1088:1110	anti-tuberculosis drugs	1088:1110	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	27	theme	necrosis	949:956	arg1	factor					958:963	tumor necrosis factor	943:963	tumor necrosis factor	943:963	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	11	28	theme	gut	2094:2096	arg1	microflora					2098:2107	the gut microflora	2090:2107	the gut microflora	2090:2107	The results prove that Lactobacillus casei can enhance the intestinal barrier and change the composition of the gut microflora.
37298396	6	29	theme	Desulfovibrio	1176:1188	arg1	abundance					1145:1153	the abundance	1141:1153	the abundance of Lactobacillus and Desulfovibrio	1141:1188	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	0	30	theme	Lactobacillus	32:44	arg1	casei					46:50	Lactobacillus casei	32:50	Lactobacillus casei	32:50	Regulation of Gut Microflora by Lactobacillus casei Zhang Attenuates Liver Injury in Mice Caused by Anti-Tuberculosis Drugs.
37298396	8	31	theme	pathway	1688:1694	arg1	expression					1704:1713	pathway protein expression	1688:1713	pathway protein expression	1688:1713	Spearman correlation analysis indicated that Lactobacillus and Desulfovibrio were positively correlated with ZO-1 or occludin protein expression and negatively correlated with pathway protein expression.
37298396	2	32	theme	-nuclear	385:392	arg1	MyD88					442:446	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88)	368:447	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	4	33	theme	biochemical	665:675	arg1	examination					694:704	biochemical and histological examination	665:704	biochemical and histological examination	665:704	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	2	34	theme	TLR4	380:383	arg1	MyD88					442:446	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88)	368:447	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	0	35	theme	Gut	14:16	arg1	Microflora					18:27	Gut Microflora	14:27	Gut Microflora	14:27	Regulation of Gut Microflora by Lactobacillus casei Zhang Attenuates Liver Injury in Mice Caused by Anti-Tuberculosis Drugs.
37298396	5	36	theme	hepatic	1002:1008	arg1	lobules					1010:1016	recovered hepatic lobules	992:1016	recovered hepatic lobules	992:1016	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	7	37	theme	p	1464:1464	arg1	expression					1452:1461	MyD88 protein expression	1438:1461	MyD88 protein expression (p < 0.05)	1438:1472	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	7	37	theme	p	1464:1464	arg1	<					1466:1466	p < 0.05	1464:1471	p < 0.05	1464:1471	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	5	38	dep	-α	970:971	arg1	malondialdehyde					916:930	malondialdehyde	916:930	malondialdehyde (MDA)	916:936	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	MDA					933:935	MDA	933:935	MDA	933:935	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	factor					958:963	tumor necrosis factor	943:963	tumor necrosis factor	943:963	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	glutathione					897:907	glutathione	897:907	glutathione (GSH)	897:913	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	dismutase					880:888	superoxide dismutase	869:888	superoxide dismutase (SOD)	869:894	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	GSH					910:912	GSH	910:912	GSH	910:912	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	phosphatase					850:860	alkaline phosphatase	841:860	alkaline phosphatase (ALP)	841:866	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	ALP					863:865	ALP	863:865	ALP	863:865	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	38	dep	-α	970:971	arg1	SOD					891:893	SOD	891:893	SOD	891:893	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	39	theme	p	981:981	arg1	levels					973:978	alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels	841:978	alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05)	841:989	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	39	theme	p	981:981	arg1	<					983:983	p < 0.05	981:988	p < 0.05	981:988	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	11	40	theme	intestinal	2041:2050	arg1	barrier					2052:2058	the intestinal barrier	2037:2058	the intestinal barrier	2037:2058	The results prove that Lactobacillus casei can enhance the intestinal barrier and change the composition of the gut microflora.
37298396	4	41	theme	real	745:748	arg1	qRT-PCR					782:788	qRT-PCR	782:788	qRT-PCR	782:788	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	4	41	theme	real	745:748	arg1	reaction					772:779	quantitative real time polymerase chain reaction	732:779	quantitative real time polymerase chain reaction (qRT-PCR)	732:789	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	2	42	theme	gut	330:332	arg1	GM					346:347	GM	346:347	GM	346:347	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	2	42	theme	gut	330:332	arg1	microflora					334:343	gut microflora	330:343	gut microflora (GM)	330:348	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	6	43	theme	zona	1241:1244	arg1	ZO					1257:1258	zona occludens (ZO)-1	1241:1261	zona occludens (ZO)-1	1241:1261	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	11	44	theme	Lactobacillus	2005:2017	arg1	casei					2019:2023	Lactobacillus casei	2005:2023	Lactobacillus casei	2005:2023	The results prove that Lactobacillus casei can enhance the intestinal barrier and change the composition of the gut microflora.
37298396	10	45	theme	positive	1929:1936	arg1	correlations					1938:1949	positive correlations	1929:1949	positive correlations with LPS and pathway proteins	1929:1979	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	9	46	contain	had	1730:1732	arg2	relationships					1755:1767	significant negative relationships	1734:1767	significant negative relationships	1734:1767	Desulfovibrio had significant negative relationships with alanine aminotransferase (ALT) and LPS levels.
37298396	9	46	contain	had	1730:1732	arg1	Desulfovibrio					1716:1728	Desulfovibrio	1716:1728	Desulfovibrio	1716:1728	Desulfovibrio had significant negative relationships with alanine aminotransferase (ALT) and LPS levels.
37298396	4	47	theme	Western	718:724	arg1	blot					726:729	Western blot	718:729	Western blot	718:729	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	9	48	theme	negative	1746:1753	arg1	relationships					1755:1767	significant negative relationships	1734:1767	significant negative relationships	1734:1767	Desulfovibrio had significant negative relationships with alanine aminotransferase (ALT) and LPS levels.
37298396	2	49	theme	casei	288:292	arg1	effect					264:269	the protective effect	249:269	the protective effect of Lactobacillus casei (Lc)	249:297	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	6	50	theme	protein	1277:1283	arg1	expression					1285:1294	zona occludens (ZO)-1 and claudin-1 protein expression	1241:1294	zona occludens (ZO)-1 and claudin-1 protein expression	1241:1294	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	5	51	theme	reduced	1023:1029	arg1	necrosis					1042:1049	reduced hepatocyte necrosis	1023:1049	reduced hepatocyte necrosis	1023:1049	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	10	52	with	associations	1857:1868	arg1	ZO-1					1875:1878	ZO-1	1875:1878	ZO-1	1875:1878	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	52	with	associations	1857:1868	arg1	LPS					1956:1958	LPS	1956:1958	LPS	1956:1958	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	52	with	associations	1857:1868	arg1	proteins					1972:1979	pathway proteins	1964:1979	pathway proteins	1964:1979	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	52	with	associations	1857:1868	arg1	occludin					1881:1888	occludin	1881:1888	occludin	1881:1888	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	52	with	associations	1857:1868	arg1	claudin-1					1895:1903	claudin-1	1895:1903	claudin-1	1895:1903	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	1	53	theme	new	158:160	arg1	perspective					162:172	a new perspective	156:172	a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI)	156:238	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	4	54	theme	time	750:753	arg1	qRT-PCR					782:788	qRT-PCR	782:788	qRT-PCR	782:788	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	4	54	theme	time	750:753	arg1	reaction					772:779	quantitative real time polymerase chain reaction	732:779	quantitative real time polymerase chain reaction (qRT-PCR)	732:789	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	8	55	theme	Spearman	1512:1519	arg1	analysis					1533:1540	Spearman correlation analysis	1512:1540	Spearman correlation analysis	1512:1540	Spearman correlation analysis indicated that Lactobacillus and Desulfovibrio were positively correlated with ZO-1 or occludin protein expression and negatively correlated with pathway protein expression.
37298396	4	56	theme	chain	766:770	arg1	qRT-PCR					782:788	qRT-PCR	782:788	qRT-PCR	782:788	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	4	56	theme	chain	766:770	arg1	reaction					772:779	quantitative real time polymerase chain reaction	732:779	quantitative real time polymerase chain reaction (qRT-PCR)	732:789	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	3	57	theme	Lc	499:500	arg1	levels					489:494	three levels	483:494	three levels of Lc	483:500	C57BL/6J mice were given three levels of Lc intragastrically for 2 h before administering isoniazid and rifampicin for 8 weeks.
37298396	1	58	theme	anti-tuberculosis	187:203	arg1	ATDILI					232:237	ATDILI	232:237	ATDILI	232:237	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	1	58	theme	anti-tuberculosis	187:203	arg1	injury					224:229	anti-tuberculosis drug-induced liver injury	187:229	anti-tuberculosis drug-induced liver injury (ATDILI)	187:238	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	7	59	theme	MyD88	1438:1442	arg1	expression					1452:1461	MyD88 protein expression	1438:1461	MyD88 protein expression (p < 0.05)	1438:1472	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	7	59	theme	MyD88	1438:1442	arg1	<					1466:1466	p < 0.05	1464:1471	p < 0.05	1464:1471	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	6	60	theme	p	1327:1327	arg1	group					1320:1324	the model group	1310:1324	the model group (p < 0.05)	1310:1335	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	6	60	theme	p	1327:1327	arg1	<					1329:1329	p < 0.05	1327:1334	p < 0.05	1327:1334	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	1	61	theme	liver	218:222	arg1	ATDILI					232:237	ATDILI	232:237	ATDILI	232:237	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	1	61	theme	liver	218:222	arg1	injury					224:229	anti-tuberculosis drug-induced liver injury	187:229	anti-tuberculosis drug-induced liver injury (ATDILI)	187:238	The gut-liver axis may provide a new perspective for treating anti-tuberculosis drug-induced liver injury (ATDILI).
37298396	5	62	theme	tumor	943:947	arg1	factor					958:963	tumor necrosis factor	943:963	tumor necrosis factor	943:963	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	2	63	theme	protective	253:262	arg1	effect					264:269	the protective effect	249:269	the protective effect of Lactobacillus casei (Lc)	249:297	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	4	64	theme	rRNA	800:803	arg1	analyses					805:812	16S rRNA analyses	796:812	16S rRNA analyses	796:812	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	0	65	theme	Liver	69:73	arg1	Injury					75:80	Liver Injury	69:80	Liver Injury	69:80	Regulation of Gut Microflora by Lactobacillus casei Zhang Attenuates Liver Injury in Mice Caused by Anti-Tuberculosis Drugs.
37298396	10	66	contain	had	1844:1846	arg1	Bilophila					1834:1842	Bilophila	1834:1842	Bilophila	1834:1842	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	66	contain	had	1844:1846	arg2	correlations					1938:1949	positive correlations	1929:1949	positive correlations with LPS and pathway proteins	1929:1979	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	10	66	contain	had	1844:1846	arg2	associations					1857:1868	negative associations	1848:1868	negative associations with ZO-1, occludin, and claudin-1 protein expressions	1848:1923	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	5	67	theme	-α	970:971	arg1	levels					973:978	alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels	841:978	alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05)	841:989	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	67	theme	-α	970:971	arg1	<					983:983	p < 0.05	981:988	p < 0.05	981:988	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	12	68	theme	pathway	2174:2180	arg1	activation					2182:2191	TLR4-NF-κB-MyD88 pathway activation	2157:2191	TLR4-NF-κB-MyD88 pathway activation	2157:2191	Moreover, Lactobacillus casei may also inhibit TLR4-NF-κB-MyD88 pathway activation and alleviate ATDILI.
37298396	6	69	theme	Bilophila	1204:1212	arg1	abundance					1214:1222	Bilophila abundance	1204:1222	Bilophila abundance	1204:1222	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	12	70	theme	Lactobacillus	2120:2132	arg1	casei					2134:2138	Lactobacillus casei	2120:2138	Lactobacillus casei	2120:2138	Moreover, Lactobacillus casei may also inhibit TLR4-NF-κB-MyD88 pathway activation and alleviate ATDILI.
37298396	0	71	theme	Anti-Tuberculosis	100:116	arg1	Drugs					118:122	Anti-Tuberculosis Drugs	100:122	Anti-Tuberculosis Drugs	100:122	Regulation of Gut Microflora by Lactobacillus casei Zhang Attenuates Liver Injury in Mice Caused by Anti-Tuberculosis Drugs.
37298396	5	72	theme	superoxide	869:878	arg1	SOD					891:893	SOD	891:893	SOD	891:893	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	72	theme	superoxide	869:878	arg1	dismutase					880:888	superoxide dismutase	869:888	superoxide dismutase (SOD)	869:894	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	7	73	theme	pathway	1492:1498	arg1	activation					1500:1509	pathway activation	1492:1509	pathway activation	1492:1509	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	11	74	theme	microflora	2098:2107	arg1	composition					2075:2085	the composition	2071:2085	the composition of the gut microflora	2071:2107	The results prove that Lactobacillus casei can enhance the intestinal barrier and change the composition of the gut microflora.
37298396	6	75	theme	claudin-1	1267:1275	arg1	expression					1285:1294	zona occludens (ZO)-1 and claudin-1 protein expression	1241:1294	zona occludens (ZO)-1 and claudin-1 protein expression	1241:1294	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	5	76	theme	alkaline	841:848	arg1	ALP					863:865	ALP	863:865	ALP	863:865	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	5	76	theme	alkaline	841:848	arg1	phosphatase					850:860	alkaline phosphatase	841:860	alkaline phosphatase (ALP)	841:866	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	2	77	theme	factor	394:399	arg1	MyD88					442:446	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88)	368:447	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	0	78	theme	Microflora	18:27	arg1	Regulation					0:9	Regulation	0:9	Regulation of Gut Microflora by Lactobacillus casei Zhang	0:56	Regulation of Gut Microflora by Lactobacillus casei Zhang Attenuates Liver Injury in Mice Caused by Anti-Tuberculosis Drugs.
37298396	0	78	theme	Microflora	18:27	arg1	Zhang					52:56	Zhang	52:56	Zhang	52:56	Regulation of Gut Microflora by Lactobacillus casei Zhang Attenuates Liver Injury in Mice Caused by Anti-Tuberculosis Drugs.
37298396	5	79	theme	recovered	992:1000	arg1	lobules					1010:1016	recovered hepatic lobules	992:1016	recovered hepatic lobules	992:1016	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
37298396	6	80	theme	ZO	1257:1258	arg1	expression					1285:1294	zona occludens (ZO)-1 and claudin-1 protein expression	1241:1294	zona occludens (ZO)-1 and claudin-1 protein expression	1241:1294	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	7	81	theme	Lc	1351:1352	arg1	pretreatment					1354:1365	Lc pretreatment	1351:1365	Lc pretreatment	1351:1365	Furthermore, Lc pretreatment reduced the lipopolysaccharide (LPS) level and downregulated NF-κB and MyD88 protein expression (p < 0.05), thus restraining pathway activation.
37298396	2	82	theme	-κB-myeloiddifferentiationfactor	405:436	arg1	MyD88					442:446	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88)	368:447	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	2	83	theme	receptor	368:375	arg1	MyD88					442:446	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88)	368:447	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	6	84	theme	occludens	1246:1254	arg1	ZO					1257:1258	zona occludens (ZO)-1	1241:1261	zona occludens (ZO)-1	1241:1261	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	2	85	theme	NF	402:403	arg1	MyD88					442:446	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88)	368:447	receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway	368:455	Herein, the protective effect of Lactobacillus casei (Lc) was investigated by modulating gut microflora (GM) and the toll like receptor 4 (TLR4)-nuclear factor (NF)-κB-myeloiddifferentiationfactor 88 (MyD88) pathway.
37298396	4	86	theme	quantitative	732:743	arg1	qRT-PCR					782:788	qRT-PCR	782:788	qRT-PCR	782:788	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	4	86	theme	quantitative	732:743	arg1	reaction					772:779	quantitative real time polymerase chain reaction	732:779	quantitative real time polymerase chain reaction (qRT-PCR)	732:789	Blood, liver, and colon tissues, as well as cecal contents, were collected for biochemical and histological examination, as well as Western blot, quantitative real time polymerase chain reaction (qRT-PCR), and 16S rRNA analyses.
37298396	8	87	dep	ZO-1	1621:1624	arg1	expression					1646:1655	protein expression	1638:1655	protein expression	1638:1655	Spearman correlation analysis indicated that Lactobacillus and Desulfovibrio were positively correlated with ZO-1 or occludin protein expression and negatively correlated with pathway protein expression.
37298396	6	88	theme	model	1314:1318	arg1	group					1320:1324	the model group	1310:1324	the model group (p < 0.05)	1310:1335	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	6	88	theme	model	1314:1318	arg1	<					1329:1329	p < 0.05	1327:1334	p < 0.05	1327:1334	Moreover, Lc also increased the abundance of Lactobacillus and Desulfovibrio and decreased Bilophila abundance, while enhancing zona occludens (ZO)-1 and claudin-1 protein expression compared with the model group (p < 0.05).
37298396	10	89	theme	protein	1905:1911	arg1	expressions					1913:1923	protein expressions	1905:1923	protein expressions	1905:1923	In contrast, Bilophila had negative associations with ZO-1, occludin, and claudin-1 protein expressions and positive correlations with LPS and pathway proteins.
37298396	5	90	theme	liver	1064:1068	arg1	injury					1070:1075	liver injury	1064:1075	liver injury induced by anti-tuberculosis drugs	1064:1110	Lc intervention decreased alkaline phosphatase (ALP), superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and tumor necrosis factor (TNF)-α levels (p < 0.05), recovered hepatic lobules, and reduced hepatocyte necrosis to alleviate liver injury induced by anti-tuberculosis drugs.
36772046	1	0	theme	composition	294:304	arg1	terms					270:274	terms	270:274	terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin	270:469	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	1	theme	cocoa	124:128	arg1	industry					130:137	the cocoa industry	120:137	the cocoa industry	120:137	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	4	2	theme	CBS	928:930	arg1	polysaccharides					942:956	CBS cell wall polysaccharides	928:956	CBS cell wall polysaccharides	928:956	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	1	3	theme	dietary	381:387	arg1	carbohydrates					353:365	carbohydrates	353:365	carbohydrates	353:365	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	3	theme	dietary	381:387	arg1	fiber					389:393	dietary fiber	381:393	dietary fiber	381:393	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	4	theme	industry	130:137	arg1	shells					90:95	Cocoa bean shells	79:95	Cocoa bean shells (CBS)	79:101	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	4	theme	industry	130:137	arg1	by-product					106:115	a by-product	104:115	a by-product of the cocoa industry	104:137	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	4	5	theme	wall	937:940	arg1	polysaccharides					942:956	CBS cell wall polysaccharides	928:956	CBS cell wall polysaccharides	928:956	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	2	6	theme	alkaline	515:522	arg1	extraction					524:533	alkaline extraction	515:533	alkaline extraction (0.5 M or 4 M KOH)	515:552	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	3	7	theme	diverse	801:807	arg1	profile					809:815	a diverse profile	799:815	a diverse profile of neutral sugars and uronic acids	799:850	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	5	8	theme	high	1119:1122	arg1	amounts					1124:1130	high amounts	1119:1130	high amounts of lactic acid	1119:1145	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	5	8	theme	high	1119:1122	arg1	acid					1142:1145	lactic acid	1135:1145	lactic acid	1135:1145	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	2	9	theme	M	547:547	arg1	KOH					549:551	4 M KOH	545:551	4 M KOH	545:551	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	4	10	theme	growth	890:895	arg1	promotion					873:881	the promotion	869:881	the promotion of the growth of selected probiotic strains	869:925	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	2	11	theme	4	545:545	arg1	M					547:547	M	547:547	M	547:547	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	1	12	theme	Cocoa	79:83	arg1	shells					90:95	Cocoa bean shells	79:95	Cocoa bean shells (CBS)	79:101	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	12	theme	Cocoa	79:83	arg1	by-product					106:115	a by-product	104:115	a by-product of the cocoa industry	104:137	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	12	theme	Cocoa	79:83	arg1	CBS					98:100	CBS	98:100	CBS	98:100	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	4	13	theme	prebiotic	1034:1042	arg1	scores					1053:1058	the prebiotic activity scores	1030:1058	the prebiotic activity scores	1030:1058	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	4	14	theme	probiotic	909:917	arg1	strains					919:925	selected probiotic strains	900:925	selected probiotic strains	900:925	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	2	15	dep	extraction	524:533	arg1	KOH					549:551	4 M KOH	545:551	4 M KOH	545:551	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	15	dep	extraction	524:533	arg1	M					540:540	0.5 M	536:540	0.5 M	536:540	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	1	16	theme	bean	85:88	arg1	shells					90:95	Cocoa bean shells	79:95	Cocoa bean shells (CBS)	79:101	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	16	theme	bean	85:88	arg1	by-product					106:115	a by-product	104:115	a by-product of the cocoa industry	104:137	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	16	theme	bean	85:88	arg1	CBS					98:100	CBS	98:100	CBS	98:100	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	4	17	theme	selected	900:907	arg1	strains					919:925	selected probiotic strains	900:925	selected probiotic strains	900:925	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	5	18	theme	short-chain	1065:1075	arg1	profiles					1088:1095	The short-chain fatty acid profiles	1061:1095	The short-chain fatty acid profiles	1061:1095	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	5	19	dep	characterized	1102:1114	arg1	followed					1148:1155	followed	1148:1155	followed by acetic and propionic acid	1148:1184	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	2	20	theme	hemi-	688:692	arg1	polysaccharides					705:719	hemi- cellulosic polysaccharides	688:719	pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%)	597:732	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	20	theme	hemi-	688:692	arg1	%					731:731	13.9-19.4%	722:731	13.9-19.4%	722:731	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	1	21	theme	cacao	149:153	arg1	varieties					155:163	two cacao varieties	145:163	two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting)	145:246	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	5	22	theme	fatty	1077:1081	arg1	profiles					1088:1095	The short-chain fatty acid profiles	1061:1095	The short-chain fatty acid profiles	1061:1095	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	2	23	theme	wall	477:480	arg1	polysaccharides					482:496	Cell wall polysaccharides	472:496	Cell wall polysaccharides	472:496	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	3	24	theme	uronic	839:844	arg1	acids					846:850	uronic acids	839:850	uronic acids	839:850	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	4	25	theme	strains	919:925	arg1	growth					890:895	the growth	886:895	the growth of selected probiotic strains	886:925	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	5	26	theme	acid	1083:1086	arg1	profiles					1088:1095	The short-chain fatty acid profiles	1061:1095	The short-chain fatty acid profiles	1061:1095	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	2	27	theme	Cell	472:475	arg1	polysaccharides					482:496	Cell wall polysaccharides	472:496	Cell wall polysaccharides	472:496	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	1	28	dep	conditions	204:213	arg1	roasting					238:245	roasting	238:245	roasting	238:245	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	28	dep	conditions	204:213	arg1	drying					230:235	drying	230:235	drying	230:235	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	28	dep	conditions	204:213	arg1	fermentation					216:227	fermentation	216:227	fermentation	216:227	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	3	29	theme	neutral	820:826	arg1	sugars					828:833	neutral sugars	820:833	neutral sugars	820:833	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	2	30	dep	polysaccharides	604:618	arg1	arabinogalactan					661:675	arabinogalactan	661:675	arabinogalactan	661:675	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	30	dep	polysaccharides	604:618	arg1	polysaccharides					604:618	pectic polysaccharides	597:618	pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%)	597:732	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	30	dep	polysaccharides	604:618	arg1	rhamnogalacturonan					638:655	rhamnogalacturonan	638:655	rhamnogalacturonan	638:655	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	3	31	theme	sugars	828:833	arg1	profile					809:815	a diverse profile	799:815	a diverse profile of neutral sugars and uronic acids	799:850	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	0	32	theme	Bean	41:44	arg1	Polysaccharides					62:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Extraction and Characterization of Cocoa Bean Shell Cell Wall Polysaccharides.
36772046	3	33	theme	0.5	744:746	arg1	M					748:748	M	748:748	M	748:748	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	0	34	theme	Cocoa	35:39	arg1	Polysaccharides					62:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Extraction and Characterization of Cocoa Bean Shell Cell Wall Polysaccharides.
36772046	1	35	theme	great	337:341	arg1	source					343:348	a great source	335:348	a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin	335:469	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	35	theme	great	337:341	arg1	they					313:316	they	313:316	they	313:316	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	0	36	theme	Cell	52:55	arg1	Polysaccharides					62:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Extraction and Characterization of Cocoa Bean Shell Cell Wall Polysaccharides.
36772046	2	37	theme	pectic	597:602	arg1	polysaccharides					604:618	pectic polysaccharides	597:618	pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%)	597:732	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	37	theme	pectic	597:602	arg1	%					628:628	80.6-86%	621:628	80.6-86%	621:628	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	37	theme	pectic	597:602	arg1	rhamnogalacturonan					638:655	rhamnogalacturonan	638:655	rhamnogalacturonan	638:655	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	37	theme	pectic	597:602	arg1	arabinogalactan					661:675	arabinogalactan	661:675	arabinogalactan	661:675	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	0	38	theme	Shell	46:50	arg1	Polysaccharides					62:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Extraction and Characterization of Cocoa Bean Shell Cell Wall Polysaccharides.
36772046	1	39	from	varieties	155:163	arg1	shells					90:95	Cocoa bean shells	79:95	Cocoa bean shells (CBS)	79:101	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	39	from	varieties	155:163	arg1	by-product					106:115	a by-product	104:115	a by-product of the cocoa industry	104:137	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	39	from	varieties	155:163	arg1	CBS					98:100	CBS	98:100	CBS	98:100	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	3	40	theme	M	748:748	arg1	polysaccharides					754:768	0.5 M KOH polysaccharides	744:768	0.5 M KOH polysaccharides	744:768	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	1	41	theme	carbohydrates	353:365	arg1	source					343:348	a great source	335:348	a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin	335:469	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	1	41	theme	carbohydrates	353:365	arg1	they					313:316	they	313:316	they	313:316	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	3	42	theme	acids	846:850	arg1	profile					809:815	a diverse profile	799:815	a diverse profile of neutral sugars and uronic acids	799:850	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	3	43	theme	KOH	750:752	arg1	polysaccharides					754:768	0.5 M KOH polysaccharides	744:768	0.5 M KOH polysaccharides	744:768	Overall, 0.5 M KOH polysaccharides were favored having provided a diverse profile of neutral sugars and uronic acids.
36772046	1	44	theme	selected	184:191	arg1	conditions					204:213	selected processing conditions	184:213	selected processing conditions (fermentation, drying, roasting)	184:246	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	5	45	theme	propionic	1171:1179	arg1	acid					1181:1184	acetic and propionic acid	1160:1184	acetic and propionic acid	1160:1184	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	1	46	theme	processing	193:202	arg1	conditions					204:213	selected processing conditions	184:213	selected processing conditions (fermentation, drying, roasting)	184:246	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	0	47	theme	Polysaccharides	62:76	arg1	Characterization					15:30	Characterization	15:30	Characterization	15:30	Extraction and Characterization of Cocoa Bean Shell Cell Wall Polysaccharides.
36772046	0	47	theme	Polysaccharides	62:76	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction and Characterization of Cocoa Bean Shell Cell Wall Polysaccharides.
36772046	5	48	theme	acetic	1160:1165	arg1	acid					1181:1184	acetic and propionic acid	1160:1184	acetic and propionic acid	1160:1184	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	1	49	dep	varieties	155:163	arg1	obtained					169:176	obtained	169:176	obtained after selected processing conditions (fermentation, drying, roasting)	169:246	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	4	50	theme	activity	1044:1051	arg1	scores					1053:1058	the prebiotic activity scores	1030:1058	the prebiotic activity scores	1030:1058	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	2	51	theme	cellulosic	694:703	arg1	polysaccharides					705:719	hemi- cellulosic polysaccharides	688:719	pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%)	597:732	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	2	51	theme	cellulosic	694:703	arg1	%					731:731	13.9-19.4%	722:731	13.9-19.4%	722:731	Cell wall polysaccharides were isolated by alkaline extraction (0.5 M or 4 M KOH) and were found to be enriched primarily in pectic polysaccharides (80.6-86%) namely rhamnogalacturonan and arabinogalactan as well as hemi- cellulosic polysaccharides (13.9-19.4%).
36772046	5	52	theme	lactic	1135:1140	arg1	acid					1142:1145	lactic acid	1135:1145	lactic acid	1135:1145	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	0	53	theme	Wall	57:60	arg1	Polysaccharides					62:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Cocoa Bean Shell Cell Wall Polysaccharides	35:76	Extraction and Characterization of Cocoa Bean Shell Cell Wall Polysaccharides.
36772046	1	54	theme	chemical	285:292	arg1	composition					294:304	their chemical composition	279:304	their chemical composition	279:304	Cocoa bean shells (CBS), a by-product of the cocoa industry, from two cacao varieties and obtained after selected processing conditions (fermentation, drying, roasting) were characterized in terms of their chemical composition, where they were found to be a great source of carbohydrates, specifically dietary fiber, protein, ash, and polyphenols, namely quercetin, epicatechin, and catechin.
36772046	4	55	theme	cell	932:935	arg1	polysaccharides					942:956	CBS cell wall polysaccharides	928:956	CBS cell wall polysaccharides	928:956	When tested for the promotion of the growth of selected probiotic strains, CBS cell wall polysaccharides performed similarly or more than inulin and rhamnogalacturonan based on the prebiotic activity scores.
36772046	5	56	theme	acid	1142:1145	arg1	amounts					1124:1130	high amounts	1119:1130	high amounts of lactic acid	1119:1145	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36772046	5	56	theme	acid	1142:1145	arg1	acid					1142:1145	lactic acid	1135:1145	lactic acid	1135:1145	The short-chain fatty acid profiles were characterized by high amounts of lactic acid, followed by acetic and propionic acid.
36905858	1	0	theme	ultrasonic-assisted	356:374	arg1	extraction					376:385	ultrasonic-assisted extraction	356:385	ultrasonic-assisted extraction (UAE)	356:391	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	0	theme	ultrasonic-assisted	356:374	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	0	theme	ultrasonic-assisted	356:374	arg1	UAE					388:390	UAE	388:390	UAE	388:390	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	2	1	theme	damage	625:630	arg1	capacity					693:700	better comprehensive antioxidant capacity	660:700	better comprehensive antioxidant capacity	660:700	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	2	1	theme	damage	625:630	arg1	degree					615:620	greater degree	607:620	greater degree of damage to the cell wall of DPs	607:654	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	4	2	theme	DPs	1093:1095	arg1	avoidance					1080:1088	degradation avoidance	1068:1088	degradation avoidance	1068:1088	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	2	theme	DPs	1093:1095	arg1	stretching					1053:1062	conformational stretching	1038:1062	conformational stretching	1038:1062	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	3	3	contain	had	732:734	arg1	methods					724:730	Different extraction methods	703:730	Different extraction methods	703:730	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	3	contain	had	732:734	arg2	effect					747:752	no obvious effect	736:752	no obvious effect	736:752	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	1	4	theme	hot	282:284	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	4	theme	hot	282:284	arg1	HWE					313:315	HWE	313:315	HWE	313:315	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	4	theme	hot	282:284	arg1	extraction					301:310	hot water-assisted extraction	282:310	hot water-assisted extraction (HWE)	282:316	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	4	5	theme	polysaccharides	990:1004	arg1	related					1023:1029	related	1023:1029	related	1023:1029	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	5	theme	polysaccharides	990:1004	arg1	yield					1006:1010	the highest polysaccharides yield	978:1010	the highest polysaccharides yield	978:1010	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	3	6	theme	Different	703:711	arg1	methods					724:730	Different extraction methods	703:730	Different extraction methods	703:730	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	1	7	theme	water-assisted	286:299	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	7	theme	water-assisted	286:299	arg1	HWE					313:315	HWE	313:315	HWE	313:315	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	7	theme	water-assisted	286:299	arg1	extraction					301:310	hot water-assisted extraction	282:310	hot water-assisted extraction (HWE)	282:316	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	4	8	theme	UMAE	962:965	arg1	method					967:972	UMAE method	962:972	UMAE method	962:972	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	3	9	theme	extraction	713:722	arg1	methods					724:730	Different extraction methods	703:730	Different extraction methods	703:730	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	4	10	theme	ultrasonic	1186:1195	arg1	action					1162:1167	the simultaneous action	1145:1167	the simultaneous action of microwave and ultrasonic	1145:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	0	11	theme	difference	122:131	arg1	mechanisms					133:142	The difference mechanisms	118:142	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.	0:191	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	2	12	theme	greater	607:613	arg1	degree					615:620	greater degree	607:620	greater degree of damage to the cell wall of DPs	607:654	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	2	13	theme	DPs	652:654	arg1	wall					644:647	the cell wall	635:647	the cell wall of DPs	635:654	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	4	14	theme	simultaneous	1149:1160	arg1	action					1162:1167	the simultaneous action	1145:1167	the simultaneous action of microwave and ultrasonic	1145:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	3	15	theme	monosaccharide	837:850	arg1	composition					852:862	monosaccharide composition	837:862	monosaccharide composition	837:862	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	15	theme	monosaccharide	837:850	arg1	types					761:765	the types	757:765	the types of glycosidic bonds and sugar rings	757:801	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	0	16	theme	single	152:157	arg1	extraction					181:190	single and combined assisted extraction	152:190	single and combined assisted extraction	152:190	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	4	17	contain	had	974:976	arg2	yield					1006:1010	the highest polysaccharides yield	978:1010	the highest polysaccharides yield	978:1010	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	17	contain	had	974:976	arg2	related					1023:1029	related	1023:1029	related	1023:1029	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	17	contain	had	974:976	arg1	DPs					954:956	DPs	954:956	DPs for UMAE method	954:972	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	18	dep	stretching	1053:1062	arg1	the					1034:1036	the	1034:1036	the	1034:1036	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	1	19	theme	chemical	462:469	arg1	structures					471:480	chemical structures	462:480	chemical structures	462:480	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	5	20	theme	functional	1312:1321	arg1	industry					1328:1335	the functional food industry	1308:1335	the functional food industry	1308:1335	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	2	21	theme	cell	639:642	arg1	wall					644:647	the cell wall	635:647	the cell wall of DPs	635:654	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	5	22	theme	good	1250:1253	arg1	potential					1255:1263	good potential	1250:1263	good potential for modification and application of DPs	1250:1303	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	1	23	theme	study	213:217	arg1	purpose					197:203	The purpose	193:203	The purpose of this study	193:217	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	3	24	theme	obvious	739:745	arg1	effect					747:752	no obvious effect	736:752	no obvious effect	736:752	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	4	25	theme	highest	982:988	arg1	related					1023:1029	related	1023:1029	related	1023:1029	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	25	theme	highest	982:988	arg1	yield					1006:1010	the highest polysaccharides yield	978:1010	the highest polysaccharides yield	978:1010	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	3	26	theme	molecular	915:923	arg1	conformation					925:936	molecular conformation	915:936	molecular conformation	915:936	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	0	27	theme	Ultrasonic-microwave-assisted	0:28	arg1	extraction					30:39	Ultrasonic-microwave-assisted extraction	0:39	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.	0:191	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	4	28	from	stretching	1053:1062	arg1	components					1128:1137	the higher molecular weight components	1100:1137	the higher molecular weight components under the simultaneous action of microwave and ultrasonic	1100:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	1	29	theme	antioxidant	486:496	arg1	activity					498:505	antioxidant activity	486:505	antioxidant activity	486:505	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	5	30	contain	has	1246:1248	arg1	technology					1235:1244	the UMAE technology	1226:1244	the UMAE technology	1226:1244	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	5	30	contain	has	1246:1248	arg2	potential					1255:1263	good potential	1250:1263	good potential for modification and application of DPs	1250:1303	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	4	31	theme	weight	1121:1126	arg1	components					1128:1137	the higher molecular weight components	1100:1137	the higher molecular weight components under the simultaneous action of microwave and ultrasonic	1100:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	2	32	theme	antioxidant	681:691	arg1	capacity					693:700	better comprehensive antioxidant capacity	660:700	better comprehensive antioxidant capacity	660:700	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	0	33	theme	combined	163:170	arg1	extraction					181:190	single and combined assisted extraction	152:190	single and combined assisted extraction	152:190	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	2	34	theme	comprehensive	667:679	arg1	capacity					693:700	better comprehensive antioxidant capacity	660:700	better comprehensive antioxidant capacity	660:700	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	1	35	dep	methods	273:279	arg1	extraction					429:438	ultrasonic-microwave- assisted extraction	398:438	ultrasonic-microwave- assisted extraction (UAME)	398:445	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	MAE					350:352	MAE	350:352	MAE	350:352	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	UAE					388:390	UAE	388:390	UAE	388:390	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	HWE					313:315	HWE	313:315	HWE	313:315	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	UAME					441:444	UAME	441:444	UAME	441:444	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	extraction					376:385	ultrasonic-assisted extraction	356:385	ultrasonic-assisted extraction (UAE)	356:391	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	extraction					301:310	hot water-assisted extraction	282:310	hot water-assisted extraction (HWE)	282:316	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	35	dep	methods	273:279	arg1	extraction					338:347	microwave-assisted extraction	319:347	microwave-assisted extraction (MAE)	319:353	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	4	36	theme	molecular	1111:1119	arg1	components					1128:1137	the higher molecular weight components	1100:1137	the higher molecular weight components under the simultaneous action of microwave and ultrasonic	1100:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	37	theme	conformational	1038:1051	arg1	stretching					1053:1062	conformational stretching	1038:1062	conformational stretching	1038:1062	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	1	38	theme	ultrasonic-microwave-	398:418	arg1	extraction					429:438	ultrasonic-microwave- assisted extraction	398:438	ultrasonic-microwave- assisted extraction (UAME)	398:445	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	38	theme	ultrasonic-microwave-	398:418	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	38	theme	ultrasonic-microwave-	398:418	arg1	UAME					441:444	UAME	441:444	UAME	441:444	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	39	theme	microwave-assisted	319:336	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	39	theme	microwave-assisted	319:336	arg1	MAE					350:352	MAE	350:352	MAE	350:352	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	39	theme	microwave-assisted	319:336	arg1	extraction					338:347	microwave-assisted extraction	319:347	microwave-assisted extraction (MAE)	319:353	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	2	40	theme	research	559:566	arg1	results					568:574	The research results	555:574	The research results	555:574	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	1	41	theme	assisted	420:427	arg1	extraction					429:438	ultrasonic-microwave- assisted extraction	398:438	ultrasonic-microwave- assisted extraction (UAME)	398:445	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	41	theme	assisted	420:427	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	41	theme	assisted	420:427	arg1	UAME					441:444	UAME	441:444	UAME	441:444	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	2	42	contain	had	603:605	arg2	capacity					693:700	better comprehensive antioxidant capacity	660:700	better comprehensive antioxidant capacity	660:700	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	2	42	contain	had	603:605	arg1	treatment					593:601	UMAE treatment	588:601	UMAE treatment	588:601	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	2	42	contain	had	603:605	arg2	degree					615:620	greater degree	607:620	greater degree of damage to the cell wall of DPs	607:654	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	5	43	theme	UMAE	1230:1233	arg1	technology					1235:1244	the UMAE technology	1226:1244	the UMAE technology	1226:1244	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	0	44	theme	antioxidant	55:65	arg1	activity					67:74	antioxidant activity	55:74	antioxidant activity of Dictyophora indusiata polysaccharides	55:115	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	3	45	theme	sugar	791:795	arg1	rings					797:801	sugar rings	791:801	sugar rings	791:801	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	0	46	theme	assisted	172:179	arg1	extraction					181:190	single and combined assisted extraction	152:190	single and combined assisted extraction	152:190	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	1	47	from	effects	241:247	arg1	yield					455:459	the yield	451:459	the yield	451:459	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	47	from	effects	241:247	arg1	structures					471:480	chemical structures	462:480	chemical structures	462:480	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	47	from	effects	241:247	arg1	activity					498:505	antioxidant activity	486:505	antioxidant activity	486:505	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	0	48	theme	Dictyophora	79:89	arg1	polysaccharides					101:115	Dictyophora indusiata polysaccharides	79:115	Dictyophora indusiata polysaccharides	79:115	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	3	49	theme	rings	797:801	arg1	composition					821:831	similar chemical composition	804:831	similar chemical composition	804:831	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	49	theme	rings	797:801	arg1	composition					852:862	monosaccharide composition	837:862	monosaccharide composition	837:862	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	49	theme	rings	797:801	arg1	types					761:765	the types	757:765	the types of glycosidic bonds and sugar rings	757:801	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	50	theme	glycosidic	770:779	arg1	bonds					781:785	glycosidic bonds	770:785	glycosidic bonds	770:785	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	1	51	theme	Dictyophora	510:520	arg1	DPs					549:551	DPs	549:551	DPs	549:551	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	51	theme	Dictyophora	510:520	arg1	polysaccharides					532:546	Dictyophora indusiata polysaccharides	510:546	Dictyophora indusiata polysaccharides (DPs)	510:552	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	5	52	theme	food	1323:1326	arg1	industry					1328:1335	the functional food industry	1308:1335	the functional food industry	1308:1335	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	3	53	theme	bonds	781:785	arg1	composition					821:831	similar chemical composition	804:831	similar chemical composition	804:831	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	53	theme	bonds	781:785	arg1	composition					852:862	monosaccharide composition	837:862	monosaccharide composition	837:862	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	53	theme	bonds	781:785	arg1	types					761:765	the types	757:765	the types of glycosidic bonds and sugar rings	757:801	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	1	54	theme	indusiata	522:530	arg1	DPs					549:551	DPs	549:551	DPs	549:551	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	54	theme	indusiata	522:530	arg1	polysaccharides					532:546	Dictyophora indusiata polysaccharides	510:546	Dictyophora indusiata polysaccharides (DPs)	510:552	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	3	55	theme	absolute	880:887	arg1	Mw					907:908	Mw	907:908	Mw	907:908	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	55	theme	absolute	880:887	arg1	weight					899:904	different absolute molecular weight	870:904	different absolute molecular weight (Mw)	870:909	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	2	56	theme	UMAE	588:591	arg1	treatment					593:601	UMAE treatment	588:601	UMAE treatment	588:601	The research results showed that UMAE treatment had greater degree of damage to the cell wall of DPs and better comprehensive antioxidant capacity.
36905858	1	57	theme	polysaccharides	532:546	arg1	yield					455:459	the yield	451:459	the yield	451:459	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	57	theme	polysaccharides	532:546	arg1	structures					471:480	chemical structures	462:480	chemical structures	462:480	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	57	theme	polysaccharides	532:546	arg1	activity					498:505	antioxidant activity	486:505	antioxidant activity	486:505	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	3	58	theme	molecular	889:897	arg1	Mw					907:908	Mw	907:908	Mw	907:908	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	58	theme	molecular	889:897	arg1	weight					899:904	different absolute molecular weight	870:904	different absolute molecular weight (Mw)	870:909	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	1	59	theme	different	252:260	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	59	theme	different	252:260	arg1	extraction					429:438	ultrasonic-microwave- assisted extraction	398:438	ultrasonic-microwave- assisted extraction (UAME)	398:445	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	59	theme	different	252:260	arg1	extraction					301:310	hot water-assisted extraction	282:310	hot water-assisted extraction (HWE)	282:316	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	59	theme	different	252:260	arg1	extraction					338:347	microwave-assisted extraction	319:347	microwave-assisted extraction (MAE)	319:353	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	59	theme	different	252:260	arg1	extraction					376:385	ultrasonic-assisted extraction	356:385	ultrasonic-assisted extraction (UAE)	356:391	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	4	60	from	avoidance	1080:1088	arg1	components					1128:1137	the higher molecular weight components	1100:1137	the higher molecular weight components under the simultaneous action of microwave and ultrasonic	1100:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	4	61	theme	higher	1104:1109	arg1	components					1128:1137	the higher molecular weight components	1100:1137	the higher molecular weight components under the simultaneous action of microwave and ultrasonic	1100:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	1	62	theme	extraction	262:271	arg1	methods					273:279	different extraction methods	252:279	different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME))	252:446	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	62	theme	extraction	262:271	arg1	extraction					429:438	ultrasonic-microwave- assisted extraction	398:438	ultrasonic-microwave- assisted extraction (UAME)	398:445	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	62	theme	extraction	262:271	arg1	extraction					301:310	hot water-assisted extraction	282:310	hot water-assisted extraction (HWE)	282:316	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	62	theme	extraction	262:271	arg1	extraction					338:347	microwave-assisted extraction	319:347	microwave-assisted extraction (MAE)	319:353	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	1	62	theme	extraction	262:271	arg1	extraction					376:385	ultrasonic-assisted extraction	356:385	ultrasonic-assisted extraction (UAE)	356:391	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	0	63	theme	polysaccharides	101:115	arg1	activity					67:74	antioxidant activity	55:74	antioxidant activity of Dictyophora indusiata polysaccharides	55:115	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	5	64	theme	DPs	1301:1303	arg1	application					1286:1296	application	1286:1296	application	1286:1296	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	5	64	theme	DPs	1301:1303	arg1	modification					1269:1280	modification	1269:1280	modification	1269:1280	These findings suggest that the UMAE technology has good potential for modification and application of DPs in the functional food industry.
36905858	0	65	dep	extraction	30:39	arg1	mechanisms					133:142	The difference mechanisms	118:142	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.	0:191	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	4	66	theme	microwave	1172:1180	arg1	action					1162:1167	the simultaneous action	1145:1167	the simultaneous action of microwave and ultrasonic	1145:1195	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	3	67	theme	different	870:878	arg1	Mw					907:908	Mw	907:908	Mw	907:908	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	67	theme	different	870:878	arg1	weight					899:904	different absolute molecular weight	870:904	different absolute molecular weight (Mw)	870:909	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	1	68	theme	methods	273:279	arg1	effects					241:247	the effects	237:247	the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs)	237:552	The purpose of this study is to investigate the effects of different extraction methods (hot water-assisted extraction (HWE), microwave-assisted extraction (MAE), ultrasonic-assisted extraction (UAE), and ultrasonic-microwave- assisted extraction (UAME)) on the yield, chemical structures and antioxidant activity of Dictyophora indusiata polysaccharides (DPs).
36905858	0	69	theme	indusiata	91:99	arg1	polysaccharides					101:115	Dictyophora indusiata polysaccharides	79:115	Dictyophora indusiata polysaccharides	79:115	Ultrasonic-microwave-assisted extraction for enhancing antioxidant activity of Dictyophora indusiata polysaccharides: The difference mechanisms between single and combined assisted extraction.
36905858	3	70	theme	chemical	812:819	arg1	composition					821:831	similar chemical composition	804:831	similar chemical composition	804:831	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	70	theme	chemical	812:819	arg1	types					761:765	the types	757:765	the types of glycosidic bonds and sugar rings	757:801	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	4	71	theme	degradation	1068:1078	arg1	avoidance					1080:1088	degradation avoidance	1068:1088	degradation avoidance	1068:1088	In particular, DPs for UMAE method had the highest polysaccharides yield, which was related to the conformational stretching and degradation avoidance of DPs in the higher molecular weight components under the simultaneous action of microwave and ultrasonic.
36905858	3	72	theme	similar	804:810	arg1	composition					821:831	similar chemical composition	804:831	similar chemical composition	804:831	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36905858	3	72	theme	similar	804:810	arg1	types					761:765	the types	757:765	the types of glycosidic bonds and sugar rings	757:801	Different extraction methods had no obvious effect on the types of glycosidic bonds and sugar rings, similar chemical composition and monosaccharide composition, with different absolute molecular weight (Mw) and molecular conformation.
36091780	9	0	theme	effective	1568:1576	arg1	therapy					1578:1584	a novel effective therapy	1560:1584	a novel effective therapy for AD	1560:1591	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	6	1	theme	topical	1061:1067	arg1	application					1069:1079	the topical application	1057:1079	the topical application	1057:1079	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	6	2	theme	TFPS	888:891	arg1	administration					870:883	oral administration	865:883	oral administration of TFPS	865:891	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	6	2	theme	TFPS	888:891	arg1	application					849:859	topical application	841:859	topical application	841:859	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	7	3	theme	TFPS	1086:1089	arg1	treatment					1091:1099	The TFPS treatment	1082:1099	The TFPS treatment	1082:1099	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	3	4	theme	antioxidant	531:541	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	1	5	theme	incidence	247:255	arg1	worldwide					257:265	a high incidence worldwide	240:265	a high incidence worldwide	240:265	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	3	6	theme	anti-tumor	519:528	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	1	7	theme	severe	171:176	arg1	pruritus					178:185	severe pruritus	171:185	severe pruritus	171:185	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	9	8	theme	underlying	1599:1608	arg1	mechanisms					1610:1619	underlying mechanisms	1599:1619	underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota	1599:1750	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	3	9	theme	neuroprotective	563:577	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	5	10	from	effects	732:738	arg1	AD					785:786	AD	785:786	AD	785:786	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	3	11	theme	anti-inflammatory	544:560	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	4	12	from	effects	635:641	arg1	treatment					649:657	AD treatment	646:657	AD treatment	646:657	However, their effects on AD treatment have never been investigated.
36091780	5	13	theme	-induced	818:825	arg1	mice					830:833	dinitrofluorobenzene (DNFB)-induced AD mice	791:833	dinitrofluorobenzene (DNFB)-induced AD mice	791:833	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	4	14	theme	AD	646:647	arg1	treatment					649:657	AD treatment	646:657	AD treatment	646:657	However, their effects on AD treatment have never been investigated.
36091780	3	15	theme	hypolipidemic	597:609	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	6	16	theme	water	927:931	arg1	loss					933:936	transdermal water loss	915:936	transdermal water loss	915:936	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	3	17	theme	hypoglycemic	580:591	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	1	18	theme	Atopic	130:135	arg1	dermatitis					137:146	Atopic dermatitis	130:146	Atopic dermatitis (AD)	130:151	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	1	18	theme	Atopic	130:135	arg1	AD					149:150	AD	149:150	AD	149:150	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	9	19	theme	response	1662:1669	arg1	regulation					1641:1650	the regulation	1637:1650	the regulation of immune response	1637:1669	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	9	19	theme	response	1662:1669	arg1	improvement					1676:1686	improvement	1676:1686	improvement of both metabolism and the composition of intestinal microbiota	1676:1750	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	8	20	theme	metabolomics	1233:1244	arg1	amplicons					1273:1281	the non-targeted metabolomics and sequencing of 16S rDNA amplicons	1216:1281	the non-targeted metabolomics and sequencing of 16S rDNA amplicons	1216:1281	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	8	21	theme	sequencing	1250:1259	arg1	amplicons					1273:1281	the non-targeted metabolomics and sequencing of 16S rDNA amplicons	1216:1281	the non-targeted metabolomics and sequencing of 16S rDNA amplicons	1216:1281	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	7	22	theme	regulatory	1156:1165	arg1	cells					1169:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	1	23	contain	has	236:238	arg1	dermatitis					137:146	Atopic dermatitis	130:146	Atopic dermatitis (AD)	130:151	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	1	23	contain	has	236:238	arg2	worldwide					257:265	a high incidence worldwide	240:265	a high incidence worldwide	240:265	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	1	23	contain	has	236:238	arg1	AD					149:150	AD	149:150	AD	149:150	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	6	24	theme	AD	978:979	arg1	mice					981:984	AD mice	978:984	AD mice	978:984	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	7	25	from	proportion	1115:1124	arg1	nodes					1195:1199	mesenteric lymph nodes	1178:1199	mesenteric lymph nodes	1178:1199	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	8	26	theme	16S	1264:1266	arg1	rDNA					1268:1271	16S rDNA	1264:1271	16S rDNA	1264:1271	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	5	27	theme	administration	759:772	arg1	effects					732:738	the therapeutic effects	716:738	the therapeutic effects of topical or oral administration of TFPS on AD	716:786	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	9	28	theme	metabolism	1696:1705	arg1	regulation					1641:1650	the regulation	1637:1650	the regulation of immune response	1637:1669	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	9	28	theme	metabolism	1696:1705	arg1	improvement					1676:1686	improvement	1676:1686	improvement of both metabolism and the composition of intestinal microbiota	1676:1750	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	5	29	theme	therapeutic	720:730	arg1	effects					732:738	the therapeutic effects	716:738	the therapeutic effects of topical or oral administration of TFPS on AD	716:786	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	2	30	theme	Recent	268:273	arg1	evidence					275:282	Recent evidence	268:282	Recent evidence	268:282	Recent evidence has shown that the modulation of gut microbiota is crucial for alleviating clinical symptoms of AD.
36091780	0	31	from	dermatitis	61:70	arg1	mice					75:78	mice	75:78	mice	75:78	Tremella fuciformis polysaccharides alleviate induced atopic dermatitis in mice by regulating immune response and gut microbiota.
36091780	2	32	theme	AD	380:381	arg1	symptoms					368:375	clinical symptoms	359:375	clinical symptoms of AD	359:381	Recent evidence has shown that the modulation of gut microbiota is crucial for alleviating clinical symptoms of AD.
36091780	0	33	theme	gut	114:116	arg1	microbiota					118:127	gut microbiota	114:127	gut microbiota	114:127	Tremella fuciformis polysaccharides alleviate induced atopic dermatitis in mice by regulating immune response and gut microbiota.
36091780	0	34	theme	Tremella	0:7	arg1	polysaccharides					20:34	Tremella fuciformis polysaccharides	0:34	Tremella fuciformis polysaccharides	0:34	Tremella fuciformis polysaccharides alleviate induced atopic dermatitis in mice by regulating immune response and gut microbiota.
36091780	9	35	theme	microbiota	1741:1750	arg1	metabolism					1696:1705	metabolism	1696:1705	metabolism	1696:1705	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	9	35	theme	microbiota	1741:1750	arg1	composition					1715:1725	the composition	1711:1725	the composition of intestinal microbiota	1711:1750	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	3	36	theme	fuciformis	393:402	arg1	TFPS					421:424	TFPS	421:424	TFPS	421:424	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	36	theme	fuciformis	393:402	arg1	polysaccharides					404:418	Tremella fuciformis polysaccharides	384:418	Tremella fuciformis polysaccharides (TFPS)	384:425	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	7	37	theme	CD25	1137:1140	arg1	cells					1169:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	0	38	theme	induced	46:52	arg1	dermatitis					61:70	induced atopic dermatitis	46:70	induced atopic dermatitis in mice	46:78	Tremella fuciformis polysaccharides alleviate induced atopic dermatitis in mice by regulating immune response and gut microbiota.
36091780	5	39	theme	TFPS	777:780	arg1	administration					759:772	topical or oral administration	743:772	administration	759:772	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	2	40	theme	microbiota	321:330	arg1	crucial					335:341	crucial	335:341	crucial	335:341	Recent evidence has shown that the modulation of gut microbiota is crucial for alleviating clinical symptoms of AD.
36091780	2	40	theme	microbiota	321:330	arg1	modulation					303:312	the modulation	299:312	the modulation of gut microbiota	299:330	Recent evidence has shown that the modulation of gut microbiota is crucial for alleviating clinical symptoms of AD.
36091780	9	41	theme	TFPS	1540:1543	arg1	administration					1522:1535	the oral administration	1513:1535	the oral administration of TFPS	1513:1543	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	1	42	theme	skin	210:213	arg1	dysfunction					223:233	skin barrier dysfunction	210:233	skin barrier dysfunction	210:233	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	8	43	theme	microbiota	1371:1380	arg1	composition					1352:1362	composition	1352:1362	composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS	1352:1472	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	9	44	theme	novel	1562:1566	arg1	therapy					1578:1584	a novel effective therapy	1560:1584	a novel effective therapy for AD	1560:1591	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	7	45	theme	mesenteric	1178:1187	arg1	nodes					1195:1199	mesenteric lymph nodes	1178:1199	mesenteric lymph nodes	1178:1199	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	6	46	from	improvement	900:910	arg1	edema					969:973	ear edema	965:973	ear edema	965:973	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	6	46	from	improvement	900:910	arg1	mice					981:984	AD mice	978:984	AD mice	978:984	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	6	46	from	improvement	900:910	arg1	loss					933:936	transdermal water loss	915:936	transdermal water loss	915:936	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	6	46	from	improvement	900:910	arg1	thickening					949:958	epidermal thickening	939:958	epidermal thickening	939:958	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	5	47	theme	oral	754:757	arg1	administration					759:772	topical or oral administration	743:772	administration	759:772	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	3	48	theme	activities	482:491	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	48	theme	activities	482:491	arg1	variety					460:466	a variety	458:466	a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	458:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	48	theme	activities	482:491	arg1	activities					482:491	biological activities	471:491	biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	471:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	7	49	theme	cells	1169:1173	arg1	proportion					1115:1124	the proportion	1111:1124	the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes	1111:1199	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	1	50	theme	high	242:245	arg1	worldwide					257:265	a high incidence worldwide	240:265	a high incidence worldwide	240:265	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	6	51	theme	oral	865:868	arg1	administration					870:883	oral administration	865:883	oral administration of TFPS	865:891	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	7	52	theme	CD4	1129:1131	arg1	cells					1169:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	1	53	theme	immune	188:193	arg1	imbalance					195:203	immune imbalance	188:203	immune imbalance	188:203	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	6	54	theme	epidermal	939:947	arg1	thickening					949:958	epidermal thickening	939:958	epidermal thickening	939:958	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	2	55	theme	gut	317:319	arg1	microbiota					321:330	gut microbiota	317:330	gut microbiota	317:330	Recent evidence has shown that the modulation of gut microbiota is crucial for alleviating clinical symptoms of AD.
36091780	8	56	from	composition	1352:1362	arg1	mice					1385:1388	mice	1385:1388	mice	1385:1388	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	5	57	theme	AD	827:828	arg1	mice					830:833	dinitrofluorobenzene (DNFB)-induced AD mice	791:833	dinitrofluorobenzene (DNFB)-induced AD mice	791:833	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	3	58	contain	have	453:456	arg1	TFPS					421:424	TFPS	421:424	TFPS	421:424	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	58	contain	have	453:456	arg2	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	58	contain	have	453:456	arg2	activities					482:491	biological activities	471:491	biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	471:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	58	contain	have	453:456	arg2	variety					460:466	a variety	458:466	a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	458:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	58	contain	have	453:456	arg1	polysaccharides					404:418	Tremella fuciformis polysaccharides	384:418	Tremella fuciformis polysaccharides (TFPS)	384:425	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	6	59	theme	transdermal	915:925	arg1	loss					933:936	transdermal water loss	915:936	transdermal water loss	915:936	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	9	60	theme	immune	1655:1660	arg1	response					1662:1669	immune response	1655:1669	immune response	1655:1669	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	8	61	theme	AD-like	1414:1420	arg1	disorder					1422:1429	AD-like disorder	1414:1429	AD-like disorder	1414:1429	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	8	62	theme	non-targeted	1220:1231	arg1	metabolomics					1233:1244	the non-targeted metabolomics	1216:1244	the non-targeted metabolomics	1216:1244	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	5	63	theme	topical	743:749	arg1	administration					759:772	topical or oral administration	743:772	administration	759:772	In this study, we compared the therapeutic effects of topical or oral administration of TFPS on AD in dinitrofluorobenzene (DNFB)-induced AD mice.
36091780	7	64	theme	Foxp3	1146:1150	arg1	cells					1169:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	0	65	theme	immune	94:99	arg1	response					101:108	immune response	94:108	immune response	94:108	Tremella fuciformis polysaccharides alleviate induced atopic dermatitis in mice by regulating immune response and gut microbiota.
36091780	6	66	theme	ear	965:967	arg1	edema					969:973	ear edema	965:973	ear edema	965:973	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	8	67	theme	rDNA	1268:1271	arg1	sequencing					1250:1259	sequencing	1250:1259	sequencing of 16S rDNA	1250:1271	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	8	67	theme	rDNA	1268:1271	arg1	metabolomics					1233:1244	the non-targeted metabolomics	1216:1244	the non-targeted metabolomics	1216:1244	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	9	68	theme	composition	1715:1725	arg1	regulation					1641:1650	the regulation	1637:1650	the regulation of immune response	1637:1669	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	9	68	theme	composition	1715:1725	arg1	improvement					1676:1686	improvement	1676:1686	improvement of both metabolism and the composition of intestinal microbiota	1676:1750	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	8	69	theme	TFPS	1469:1472	arg1	administration					1451:1464	oral administration	1446:1464	oral administration of TFPS	1446:1472	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	0	70	theme	fuciformis	9:18	arg1	polysaccharides					20:34	Tremella fuciformis polysaccharides	0:34	Tremella fuciformis polysaccharides	0:34	Tremella fuciformis polysaccharides alleviate induced atopic dermatitis in mice by regulating immune response and gut microbiota.
36091780	8	71	theme	oral	1446:1449	arg1	administration					1451:1464	oral administration	1446:1464	oral administration of TFPS	1446:1472	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	6	72	theme	better	1036:1041	arg1	efficacy					1043:1050	significantly better efficacy	1022:1050	significantly better efficacy than the topical application	1022:1079	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	9	73	theme	intestinal	1730:1739	arg1	microbiota					1741:1750	intestinal microbiota	1730:1750	intestinal microbiota	1730:1750	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	2	74	theme	clinical	359:366	arg1	symptoms					368:375	clinical symptoms	359:375	clinical symptoms of AD	359:381	Recent evidence has shown that the modulation of gut microbiota is crucial for alleviating clinical symptoms of AD.
36091780	8	75	theme	metabolite	1309:1318	arg1	modulation					1320:1329	metabolite modulation	1309:1329	metabolite modulation	1309:1329	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	0	76	theme	atopic	54:59	arg1	dermatitis					61:70	induced atopic dermatitis	46:70	induced atopic dermatitis in mice	46:78	Tremella fuciformis polysaccharides alleviate induced atopic dermatitis in mice by regulating immune response and gut microbiota.
36091780	6	77	theme	topical	841:847	arg1	application					849:859	topical application	841:859	topical application	841:859	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	9	78	theme	oral	1517:1520	arg1	administration					1522:1535	the oral administration	1513:1535	the oral administration of TFPS	1513:1543	Collectively, these data suggest that the oral administration of TFPS may constitute a novel effective therapy for AD, with underlying mechanisms associated with the regulation of immune response, and improvement of both metabolism and the composition of intestinal microbiota.
36091780	6	79	theme	oral	995:998	arg1	administration					1000:1013	the oral administration	991:1013	the oral administration	991:1013	Both topical application and oral administration of TFPS led to improvement on transdermal water loss, epidermal thickening, and ear edema in AD mice, but the oral administration showed significantly better efficacy than the topical application.
36091780	3	80	theme	Tremella	384:391	arg1	TFPS					421:424	TFPS	421:424	TFPS	421:424	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	80	theme	Tremella	384:391	arg1	polysaccharides					404:418	Tremella fuciformis polysaccharides	384:418	Tremella fuciformis polysaccharides (TFPS)	384:425	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	81	theme	biological	471:480	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	3	81	theme	biological	471:480	arg1	activities					482:491	biological activities	471:491	biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	471:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	7	82	theme	lymph	1189:1193	arg1	nodes					1195:1199	mesenteric lymph nodes	1178:1199	mesenteric lymph nodes	1178:1199	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
36091780	1	83	theme	barrier	215:221	arg1	dysfunction					223:233	skin barrier dysfunction	210:233	skin barrier dysfunction	210:233	Atopic dermatitis (AD), characterized by severe pruritus, immune imbalance, and skin barrier dysfunction, has a high incidence worldwide.
36091780	8	84	theme	gut	1367:1369	arg1	microbiota					1371:1380	gut microbiota	1367:1380	gut microbiota	1367:1380	Additionally, the non-targeted metabolomics and sequencing of 16S rDNA amplicons were performed, revealing metabolite modulation in feces and changed composition of gut microbiota in mice, which were induced for AD-like disorder and treated by oral administration of TFPS.
36091780	3	85	theme	immunomodulatory	501:516	arg1	effects					611:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects	501:617	Tremella fuciformis polysaccharides (TFPS) have been demonstrated to have a variety of biological activities such as immunomodulatory, anti-tumor, antioxidant, anti-inflammatory, neuroprotective, hypoglycemic and hypolipidemic effects.
36091780	7	86	theme	T	1167:1167	arg1	cells					1169:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells	1129:1173	The TFPS treatment increased the proportion of CD4 (+) CD25 (+) Foxp3 (+) regulatory T cells in mesenteric lymph nodes.
35499019	0	0	theme	composition	92:102	arg1	regulation					43:52	Dendrobium officinale leaf polysaccharides regulation	0:52	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.	0:136	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	6	1	from	composition	867:877	arg1	feces					882:886	feces	882:886	feces	882:886	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	0	2	theme	microbiota	81:90	arg1	composition					92:102	gut microbiota composition	77:102	gut microbiota composition	77:102	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	2	3	theme	colon	357:361	arg1	progression					391:401	the colon and liver histopathological progression	353:401	the colon and liver histopathological progression	353:401	Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining.
35499019	0	4	theme	cyclophosphamide-treated	107:130	arg1	mice					132:135	cyclophosphamide-treated mice	107:135	cyclophosphamide-treated mice	107:135	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	7	5	theme	gut	959:961	arg1	microbiota					963:972	gut microbiota	959:972	gut microbiota	959:972	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	2	6	theme	spleen	297:302	arg1	indices					304:310	Thymus and spleen indices	286:310	Thymus and spleen indices	286:310	Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining.
35499019	3	7	dep	gut	505:507	arg1	atrophy					521:527	atrophy	521:527	atrophy	521:527	The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs.
35499019	3	7	dep	gut	505:507	arg1	barrier					509:515	barrier	509:515	barrier	509:515	The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs.
35499019	7	8	theme	total	1014:1018	arg1	Bacteroides					1052:1062	Bacteroides	1052:1062	Bacteroides	1052:1062	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	7	8	theme	total	1014:1018	arg1	bacteria					1020:1027	total bacteria	1014:1027	total bacteria	1014:1027	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	7	8	theme	total	1014:1018	arg1	Lachnospiraceae					1083:1097	Lachnospiraceae	1083:1097	Lachnospiraceae	1083:1097	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	7	8	theme	total	1014:1018	arg1	Lactobacillus					1065:1077	Lactobacillus	1065:1077	Lactobacillus	1065:1077	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	8	9	theme	diseases	1173:1180	arg1	treatment					1145:1153	the treatment	1141:1153	the treatment of chronic immune diseases	1141:1180	Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
35499019	2	10	theme	Thymus	286:291	arg1	indices					304:310	Thymus and spleen indices	286:310	Thymus and spleen indices	286:310	Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining.
35499019	1	11	theme	Dendrobium	188:197	arg1	DOLP					216:219	Dendrobium officinale leaf (DOLP)	188:220	Dendrobium officinale leaf (DOLP)	188:220	In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity.
35499019	5	12	theme	cytokines	795:803	arg1	expression					763:772	the expression	759:772	the expression of anti-inflammatory cytokines (IL-10)	759:811	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	6	13	theme	microbial	857:865	arg1	composition					867:877	microbial composition	857:877	microbial composition	857:877	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	1	14	theme	officinale	199:208	arg1	DOLP					216:219	Dendrobium officinale leaf (DOLP)	188:220	Dendrobium officinale leaf (DOLP)	188:220	In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity.
35499019	0	15	theme	officinale	11:20	arg1	regulation					43:52	Dendrobium officinale leaf polysaccharides regulation	0:52	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.	0:136	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	0	16	from	regulation	43:52	arg1	mice					132:135	cyclophosphamide-treated mice	107:135	cyclophosphamide-treated mice	107:135	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	1	17	theme	leaf	210:213	arg1	DOLP					216:219	Dendrobium officinale leaf (DOLP)	188:220	Dendrobium officinale leaf (DOLP)	188:220	In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity.
35499019	0	18	theme	Dendrobium	0:9	arg1	regulation					43:52	Dendrobium officinale leaf polysaccharides regulation	0:52	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.	0:136	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	6	19	theme	Short	814:818	arg1	SCFAs					839:843	SCFAs	839:843	SCFAs	839:843	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	6	19	theme	Short	814:818	arg1	acids					832:836	Short chain fatty acids	814:836	Short chain fatty acids (SCFAs) levels	814:851	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	2	20	theme	progression	391:401	arg1	alleviation					338:348	the alleviation	334:348	the alleviation of the colon and liver histopathological progression	334:401	Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining.
35499019	0	21	theme	polysaccharides	27:41	arg1	regulation					43:52	Dendrobium officinale leaf polysaccharides regulation	0:52	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.	0:136	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	8	22	theme	immune	1166:1171	arg1	diseases					1173:1180	chronic immune diseases	1158:1180	chronic immune diseases	1158:1180	Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
35499019	0	23	theme	leaf	22:25	arg1	regulation					43:52	Dendrobium officinale leaf polysaccharides regulation	0:52	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.	0:136	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	4	24	theme	inflammation	577:588	arg1	marker					567:572	marker	567:572	marker of inflammation	567:588	Cytokines levels as marker of inflammation were determined using ELISA in serum and colon.
35499019	3	25	theme	organs	539:544	arg1	gut					505:507	the gut barrier and atrophy	501:527	the gut barrier and atrophy of immune organs	501:544	The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs.
35499019	5	26	theme	TGF-	724:727	arg1	TNF-α					717:721	TNF-α	717:721	TNF-α	717:721	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	5	26	theme	TGF-	724:727	arg1	β1					729:730	TGF- β1	724:730	TGF- β1	724:730	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	1	27	used	used	226:229	arg2	polysaccharides					157:171	the polysaccharides	153:171	the polysaccharides extracted from Dendrobium officinale leaf (DOLP)	153:220	In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity.
35499019	8	28	theme	potential	1120:1128	arg1	effect					1130:1135	potential effect	1120:1135	potential effect	1120:1135	Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
35499019	6	29	from	levels	846:851	arg1	feces					882:886	feces	882:886	feces	882:886	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	2	30	theme	H&E	420:422	arg1	staining					424:431	H&E staining	420:431	H&E staining	420:431	Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining.
35499019	0	31	theme	response	64:71	arg1	regulation					43:52	Dendrobium officinale leaf polysaccharides regulation	0:52	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.	0:136	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	5	32	dep	cytokines	706:714	arg1	IL-1β					739:743	IL-1β	739:743	IL-1β	739:743	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	5	32	dep	cytokines	706:714	arg1	IL-6					733:736	IL-6	733:736	IL-6	733:736	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	5	32	dep	cytokines	706:714	arg1	TNF-α					717:721	TNF-α	717:721	TNF-α	717:721	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	5	32	dep	cytokines	706:714	arg1	β1					729:730	TGF- β1	724:730	TGF- β1	724:730	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	7	33	theme	probiotics	1033:1042	arg1	abundance					1001:1009	the relative abundance	988:1009	the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae	988:1097	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	7	34	theme	bacteria	1020:1027	arg1	abundance					1001:1009	the relative abundance	988:1009	the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae	988:1097	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	8	35	contain	has	1116:1118	arg1	DOLP					1111:1114	DOLP	1111:1114	DOLP	1111:1114	Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
35499019	8	35	contain	has	1116:1118	arg2	effect					1130:1135	potential effect	1120:1135	potential effect	1120:1135	Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
35499019	0	36	theme	immune	57:62	arg1	response					64:71	immune response	57:71	immune response	57:71	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	5	37	theme	pro-inflammatory	689:704	arg1	cytokines					706:714	pro-inflammatory cytokines	689:714	pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β)	689:744	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	6	38	theme	acids	832:836	arg1	levels					846:851	Short chain fatty acids (SCFAs) levels	814:851	Short chain fatty acids (SCFAs) levels	814:851	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	7	39	theme	relative	992:999	arg1	abundance					1001:1009	the relative abundance	988:1009	the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae	988:1097	DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae.
35499019	4	40	theme	Cytokines	547:555	arg1	levels					557:562	Cytokines levels	547:562	Cytokines levels as marker of inflammation	547:588	Cytokines levels as marker of inflammation were determined using ELISA in serum and colon.
35499019	5	41	theme	cytokines	706:714	arg1	expression					675:684	the expression	671:684	the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β)	671:744	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	6	42	theme	fatty	826:830	arg1	SCFAs					839:843	SCFAs	839:843	SCFAs	839:843	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	6	42	theme	fatty	826:830	arg1	acids					832:836	Short chain fatty acids	814:836	Short chain fatty acids (SCFAs) levels	814:851	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	8	43	theme	chronic	1158:1164	arg1	diseases					1173:1180	chronic immune diseases	1158:1180	chronic immune diseases	1158:1180	Therefore, DOLP has potential effect for the treatment of chronic immune diseases.
35499019	6	44	theme	chain	820:824	arg1	SCFAs					839:843	SCFAs	839:843	SCFAs	839:843	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	6	44	theme	chain	820:824	arg1	acids					832:836	Short chain fatty acids	814:836	Short chain fatty acids (SCFAs) levels	814:851	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
35499019	3	45	theme	immune	532:537	arg1	organs					539:544	immune organs	532:544	immune organs	532:544	The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs.
35499019	1	46	theme	immune	234:239	arg1	mice					252:255	immune deficiency mice	234:255	immune deficiency mice	234:255	In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity.
35499019	0	47	theme	gut	77:79	arg1	composition					92:102	gut microbiota composition	77:102	gut microbiota composition	77:102	Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.
35499019	3	48	theme	immunity	472:479	arg1	status					481:486	immunity status	472:486	immunity status	472:486	The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs.
35499019	2	49	theme	histopathological	373:389	arg1	progression					391:401	the colon and liver histopathological progression	353:401	the colon and liver histopathological progression	353:401	Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining.
35499019	1	50	theme	deficiency	241:250	arg1	mice					252:255	immune deficiency mice	234:255	immune deficiency mice	234:255	In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity.
35499019	5	51	theme	anti-inflammatory	777:793	arg1	IL-10					806:810	IL-10	806:810	IL-10	806:810	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	5	51	theme	anti-inflammatory	777:793	arg1	cytokines					795:803	anti-inflammatory cytokines	777:803	anti-inflammatory cytokines (IL-10)	777:811	Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10).
35499019	2	52	theme	liver	367:371	arg1	progression					391:401	the colon and liver histopathological progression	353:401	the colon and liver histopathological progression	353:401	Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining.
35499019	6	53	theme	high-throughput	917:931	arg1	sequencing					933:942	high-throughput sequencing	917:942	high-throughput sequencing	917:942	Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing.
37177147	9	0	theme	homogeneous	1416:1426	arg1	surface					1433:1439	the homogeneous film surface	1412:1439	the homogeneous film surface ensured by cross-linking	1412:1464	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	10	1	theme	highest	1615:1621	arg1	capacity					1632:1639	the highest sorption capacity	1611:1639	the highest sorption capacity	1611:1639	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	4	2	dep	acids	482:486	arg1	oxalic					507:512	oxalic	507:512	oxalic	507:512	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	4	2	dep	acids	482:486	arg1	adipic					519:524	adipic	519:524	adipic	519:524	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	4	2	dep	acids	482:486	arg1	succinic					497:504	succinic	497:504	succinic	497:504	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	4	2	dep	acids	482:486	arg1	citric					489:494	citric	489:494	citric	489:494	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	0	3	theme	Composite	101:109	arg1	Films					111:115	Composite Films	101:115	Composite Films	101:115	Preparation and Characterization of di- and Tricarboxylic Acids-Modified Arabinogalactan Plasticized Composite Films.
37177147	10	4	theme	AG	1558:1559	arg1	succinate					1561:1569	AG succinate	1558:1569	AG succinate (82.52%) obtained by lyophilization	1558:1605	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	10	4	theme	AG	1558:1559	arg1	%					1577:1577	82.52%	1572:1577	82.52%	1572:1577	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	7	5	theme	bonds	1065:1069	arg1	formation					1046:1054	The formation	1042:1054	The formation of ester bonds	1042:1069	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	5	6	theme	gel	659:661	arg1	GPC					690:692	GPC	690:692	GPC	690:692	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	6	theme	gel	659:661	arg1	chromatography					674:687	gel permeation chromatography	659:687	gel permeation chromatography (GPC)	659:693	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	10	7	dep	has	1607:1609	arg1	makes					1699:1703	makes	1699:1703	makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents	1699:1821	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	6	8	theme	inter-	955:960	arg1	bonds					981:985	additional inter- and intramolecular bonds	944:985	additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules	944:1039	It is shown that the heat treatment results in the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules.
37177147	7	9	theme	cm-1	1172:1175	arg1	range					1153:1157	the range	1149:1157	the range of 1750-1690 cm-1	1149:1175	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	5	10	theme	Fourier-transform	696:712	arg1	FTIR					737:740	FTIR	737:740	FTIR	737:740	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	10	theme	Fourier-transform	696:712	arg1	spectroscopy					723:734	Fourier-transform infrared spectroscopy	696:734	Fourier-transform infrared spectroscopy (FTIR)	696:741	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	11	theme	permeation	663:672	arg1	GPC					690:692	GPC	690:692	GPC	690:692	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	11	theme	permeation	663:672	arg1	chromatography					674:687	gel permeation chromatography	659:687	gel permeation chromatography (GPC)	659:693	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	6	12	theme	additional	944:953	arg1	bonds					981:985	additional inter- and intramolecular bonds	944:985	additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules	944:1039	It is shown that the heat treatment results in the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules.
37177147	7	13	from	spectra	1138:1144	arg1	range					1153:1157	the range	1149:1157	the range of 1750-1690 cm-1	1149:1175	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	8	14	theme	TGA	1207:1209	arg1	study					1211:1215	the TGA study	1203:1215	the TGA study	1203:1215	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	9	15	theme	films	1365:1369	arg1	study					1337:1341	The SEM study	1329:1341	The SEM study of the synthesized AG films	1329:1369	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	9	16	theme	synthesized	1350:1360	arg1	films					1365:1369	the synthesized AG films	1346:1369	the synthesized AG films	1346:1369	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	7	17	theme	absorption	1111:1120	arg1	bands					1122:1126	absorption bands	1111:1126	absorption bands	1111:1126	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	10	18	theme	water	1800:1804	arg1	agents					1816:1821	water treatment agents	1800:1821	water treatment agents	1800:1821	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	6	19	theme	intramolecular	966:979	arg1	bonds					981:985	additional inter- and intramolecular bonds	944:985	additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules	944:1039	It is shown that the heat treatment results in the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules.
37177147	4	20	theme	new	365:367	arg1	method					394:399	a new environmentally friendly method	363:399	a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials	363:555	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	10	21	theme	sorption	1510:1517	arg1	properties					1519:1528	the sorption properties	1506:1528	the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents	1506:1821	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	7	22	from	appearance	1097:1106	arg1	spectra					1138:1144	the IR spectra	1131:1144	the IR spectra in the range of 1750-1690 cm-1	1131:1175	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	5	23	theme	physicochemical	624:638	arg1	diffractometry					784:797	X-ray diffractometry	778:797	X-ray diffractometry (XRD)	778:803	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	23	theme	physicochemical	624:638	arg1	spectroscopy					723:734	Fourier-transform infrared spectroscopy	696:734	Fourier-transform infrared spectroscopy (FTIR)	696:741	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	23	theme	physicochemical	624:638	arg1	microscopy					824:833	scanning electron microscopy	806:833	scanning electron microscopy (SEM)	806:839	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	23	theme	physicochemical	624:638	arg1	analysis					762:769	thermogravimetric analysis	744:769	thermogravimetric analysis (TGA)	744:775	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	23	theme	physicochemical	624:638	arg1	chromatography					674:687	gel permeation chromatography	659:687	gel permeation chromatography (GPC)	659:693	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	23	theme	physicochemical	624:638	arg1	methods					640:646	physicochemical methods	624:646	physicochemical methods	624:646	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	23	theme	physicochemical	624:638	arg1	investigations					864:877	sorption capacity investigations	846:877	sorption capacity investigations	846:877	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	24	theme	AG	574:575	arg1	derivatives					577:587	The synthesized AG derivatives	558:587	The synthesized AG derivatives	558:587	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	10	25	theme	treatment	1806:1814	arg1	agents					1816:1821	water treatment agents	1800:1821	water treatment agents	1800:1821	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	1	26	theme	toxic	181:185	arg1	pollutants					187:196	toxic pollutants	181:196	toxic pollutants	181:196	To ensure the high quality of water, it is necessary to remove toxic pollutants.
37177147	9	27	contain	have	1407:1410	arg1	samples					1399:1405	the modified samples	1386:1405	the modified samples	1386:1405	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	9	27	contain	have	1407:1410	arg2	surface					1433:1439	the homogeneous film surface	1412:1439	the homogeneous film surface ensured by cross-linking	1412:1464	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	5	28	theme	methods	640:646	arg1	complex					613:619	a complex	611:619	a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations	611:877	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	4	29	theme	friendly	385:392	arg1	method					394:399	a new environmentally friendly method	363:399	a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials	363:555	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	8	30	theme	arabinogalactan	1274:1288	arg1	sample					1264:1269	the most thermally stable (up to 190 °C) sample	1223:1269	the most thermally stable (up to 190 °C) sample	1223:1269	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	8	30	theme	arabinogalactan	1274:1288	arg1	oxalate					1290:1296	arabinogalactan oxalate	1274:1296	arabinogalactan oxalate obtained under heat treatment	1274:1326	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	10	31	theme	eco-friendly	1737:1748	arg1	materials					1750:1758	promising eco-friendly materials	1727:1758	promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents	1727:1821	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	7	32	theme	IR	1135:1136	arg1	spectra					1138:1144	the IR spectra	1131:1144	the IR spectra in the range of 1750-1690 cm-1	1131:1175	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	4	33	theme	composite	537:545	arg1	materials					547:555	composite materials	537:555	composite materials	537:555	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	3	34	theme	efficient	276:284	arg1	materials					295:303	The efficient sorption materials	272:303	The efficient sorption materials	272:303	The efficient sorption materials are modified polysaccharides.
37177147	3	34	theme	efficient	276:284	arg1	polysaccharides					318:332	modified polysaccharides	309:332	modified polysaccharides	309:332	The efficient sorption materials are modified polysaccharides.
37177147	4	35	theme	environmentally	369:383	arg1	method					394:399	a new environmentally friendly method	363:399	a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials	363:555	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	5	36	theme	X-ray	778:782	arg1	XRD					800:802	XRD	800:802	XRD	800:802	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	36	theme	X-ray	778:782	arg1	diffractometry					784:797	X-ray diffractometry	778:797	X-ray diffractometry (XRD)	778:803	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	37	theme	scanning	806:813	arg1	microscopy					824:833	scanning electron microscopy	806:833	scanning electron microscopy (SEM)	806:839	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	37	theme	scanning	806:813	arg1	SEM					836:838	SEM	836:838	SEM	836:838	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	3	38	theme	sorption	286:293	arg1	materials					295:303	The efficient sorption materials	272:303	The efficient sorption materials	272:303	The efficient sorption materials are modified polysaccharides.
37177147	3	38	theme	sorption	286:293	arg1	polysaccharides					318:332	modified polysaccharides	309:332	modified polysaccharides	309:332	The efficient sorption materials are modified polysaccharides.
37177147	4	39	theme	polybasic	461:469	arg1	acids					482:486	polybasic carboxylic acids	461:486	larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic)	415:525	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	10	40	theme	developed	1653:1661	arg1	surface					1674:1680	the developed mesoporous surface	1649:1680	the developed mesoporous surface	1649:1680	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	5	41	theme	electron	815:822	arg1	microscopy					824:833	scanning electron microscopy	806:833	scanning electron microscopy (SEM)	806:839	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	41	theme	electron	815:822	arg1	SEM					836:838	SEM	836:838	SEM	836:838	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	10	42	theme	derivatives	1540:1550	arg1	properties					1519:1528	the sorption properties	1506:1528	the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents	1506:1821	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	5	43	theme	infrared	714:721	arg1	FTIR					737:740	FTIR	737:740	FTIR	737:740	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	43	theme	infrared	714:721	arg1	spectroscopy					723:734	Fourier-transform infrared spectroscopy	696:734	Fourier-transform infrared spectroscopy (FTIR)	696:741	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	4	44	theme	-with	455:459	arg1	acids					482:486	polybasic carboxylic acids	461:486	larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic)	415:525	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	8	45	dep	190	1256:1258	arg1	to					1253:1254	to	1253:1254	to	1253:1254	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	6	46	theme	polysaccharide	1016:1029	arg1	molecules					1031:1039	polysaccharide molecules	1016:1039	polysaccharide molecules	1016:1039	It is shown that the heat treatment results in the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules.
37177147	0	47	theme	di-	36:38	arg1	Arabinogalactan					73:87	di- and Tricarboxylic Acids-Modified Arabinogalactan	36:87	di- and Tricarboxylic Acids-Modified Arabinogalactan	36:87	Preparation and Characterization of di- and Tricarboxylic Acids-Modified Arabinogalactan Plasticized Composite Films.
37177147	10	48	contain	has	1607:1609	arg2	derivatives					1540:1550	the AG derivatives	1533:1550	the AG derivatives	1533:1550	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	10	48	contain	has	1607:1609	arg2	capacity					1632:1639	the highest sorption capacity	1611:1639	the highest sorption capacity	1611:1639	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	10	48	contain	has	1607:1609	arg1	succinate					1561:1569	AG succinate	1558:1569	AG succinate (82.52%) obtained by lyophilization	1558:1605	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	10	48	contain	has	1607:1609	arg1	%					1577:1577	82.52%	1572:1577	82.52%	1572:1577	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	10	49	theme	promising	1727:1735	arg1	materials					1750:1758	promising eco-friendly materials	1727:1758	promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents	1727:1821	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	2	50	theme	water	227:231	arg1	purification					211:222	purification	211:222	purification of water	211:231	At present, purification of water is implemented using various sorbents.
37177147	9	51	theme	SEM	1333:1335	arg1	study					1337:1341	The SEM study	1329:1341	The SEM study of the synthesized AG films	1329:1369	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	9	52	theme	modified	1390:1397	arg1	samples					1399:1405	the modified samples	1386:1405	the modified samples	1386:1405	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	0	53	theme	Acids-Modified	58:71	arg1	Arabinogalactan					73:87	di- and Tricarboxylic Acids-Modified Arabinogalactan	36:87	di- and Tricarboxylic Acids-Modified Arabinogalactan	36:87	Preparation and Characterization of di- and Tricarboxylic Acids-Modified Arabinogalactan Plasticized Composite Films.
37177147	10	54	theme	sorption	1623:1630	arg1	capacity					1632:1639	the highest sorption capacity	1611:1639	the highest sorption capacity	1611:1639	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	3	55	theme	modified	309:316	arg1	materials					295:303	The efficient sorption materials	272:303	The efficient sorption materials	272:303	The efficient sorption materials are modified polysaccharides.
37177147	3	55	theme	modified	309:316	arg1	polysaccharides					318:332	modified polysaccharides	309:332	modified polysaccharides	309:332	The efficient sorption materials are modified polysaccharides.
37177147	4	56	theme	carboxylic	471:480	arg1	acids					482:486	polybasic carboxylic acids	461:486	larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic)	415:525	In this study, we report on a new environmentally friendly method for modifying larch hemicellulose-arabinogalactan (AG)-with polybasic carboxylic acids (citric, succinic, oxalic, and adipic) to obtain composite materials.
37177147	1	57	theme	high	132:135	arg1	quality					137:143	the high quality	128:143	the high quality of water	128:152	To ensure the high quality of water, it is necessary to remove toxic pollutants.
37177147	0	58	theme	Tricarboxylic	44:56	arg1	Arabinogalactan					73:87	di- and Tricarboxylic Acids-Modified Arabinogalactan	36:87	di- and Tricarboxylic Acids-Modified Arabinogalactan	36:87	Preparation and Characterization of di- and Tricarboxylic Acids-Modified Arabinogalactan Plasticized Composite Films.
37177147	6	59	theme	heat	901:904	arg1	treatment					906:914	the heat treatment	897:914	the heat treatment	897:914	It is shown that the heat treatment results in the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules.
37177147	10	60	theme	synthesized	1709:1719	arg1	films					1721:1725	the synthesized films	1705:1725	the synthesized films	1705:1725	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	10	61	theme	drug	1771:1774	arg1	carriers					1776:1783	drug carriers	1771:1783	drug carriers	1771:1783	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	8	62	dep	stable	1242:1247	arg1	°C					1260:1261	up to 190 °C	1250:1261	up to 190 °C	1250:1261	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	5	63	theme	synthesized	562:572	arg1	derivatives					577:587	The synthesized AG derivatives	558:587	The synthesized AG derivatives	558:587	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	7	64	theme	bands	1122:1126	arg1	appearance					1097:1106	the appearance	1093:1106	the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1	1093:1175	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	8	65	theme	heat	1313:1316	arg1	treatment					1318:1326	heat treatment	1313:1326	heat treatment	1313:1326	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	8	66	theme	stable	1242:1247	arg1	sample					1264:1269	the most thermally stable (up to 190 °C) sample	1223:1269	the most thermally stable (up to 190 °C) sample	1223:1269	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	8	66	theme	stable	1242:1247	arg1	oxalate					1290:1296	arabinogalactan oxalate	1274:1296	arabinogalactan oxalate obtained under heat treatment	1274:1326	It has been found, using the TGA study, that the most thermally stable (up to 190 °C) sample is arabinogalactan oxalate obtained under heat treatment.
37177147	10	67	theme	mesoporous	1663:1672	arg1	surface					1674:1680	the developed mesoporous surface	1649:1680	the developed mesoporous surface	1649:1680	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	7	68	theme	ester	1059:1063	arg1	bonds					1065:1069	ester bonds	1059:1069	ester bonds	1059:1069	The formation of ester bonds has been confirmed by the appearance of absorption bands in the IR spectra in the range of 1750-1690 cm-1.
37177147	2	69	theme	various	254:260	arg1	sorbents					262:269	various sorbents	254:269	various sorbents	254:269	At present, purification of water is implemented using various sorbents.
37177147	1	70	theme	water	148:152	arg1	quality					137:143	the high quality	128:143	the high quality of water	128:152	To ensure the high quality of water, it is necessary to remove toxic pollutants.
37177147	6	71	theme	carboxylic	995:1004	arg1	acids					1006:1010	carboxylic acids	995:1010	carboxylic acids	995:1010	It is shown that the heat treatment results in the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules.
37177147	5	72	theme	thermogravimetric	744:760	arg1	analysis					762:769	thermogravimetric analysis	744:769	thermogravimetric analysis (TGA)	744:775	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	5	72	theme	thermogravimetric	744:760	arg1	TGA					772:774	TGA	772:774	TGA	772:774	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	9	73	theme	film	1428:1431	arg1	surface					1433:1439	the homogeneous film surface	1412:1439	the homogeneous film surface ensured by cross-linking	1412:1464	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	5	74	theme	sorption	846:853	arg1	investigations					864:877	sorption capacity investigations	846:877	sorption capacity investigations	846:877	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	9	75	theme	AG	1362:1363	arg1	films					1365:1369	the synthesized AG films	1346:1369	the synthesized AG films	1346:1369	The SEM study of the synthesized AG films has shown that the modified samples have the homogeneous film surface ensured by cross-linking.
37177147	0	76	theme	Arabinogalactan	73:87	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of di- and Tricarboxylic Acids-Modified Arabinogalactan Plasticized Composite Films.
37177147	0	76	theme	Arabinogalactan	73:87	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of di- and Tricarboxylic Acids-Modified Arabinogalactan Plasticized Composite Films.
37177147	5	77	theme	capacity	855:862	arg1	investigations					864:877	sorption capacity investigations	846:877	sorption capacity investigations	846:877	The synthesized AG derivatives have been explored by a complex of physicochemical methods, including gel permeation chromatography (GPC), Fourier-transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), X-ray diffractometry (XRD), scanning electron microscopy (SEM), and sorption capacity investigations.
37177147	10	78	theme	AG	1537:1538	arg1	derivatives					1540:1550	the AG derivatives	1533:1550	the AG derivatives	1533:1550	It has been established, when studying the sorption properties of the AG derivatives, that AG succinate (82.52%) obtained by lyophilization has the highest sorption capacity, due to the developed mesoporous surface, which, in turn, makes the synthesized films promising eco-friendly materials for use as drug carriers, sorbents, and water treatment agents.
37177147	6	79	theme	bonds	981:985	arg1	formation					931:939	the formation	927:939	the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules	927:1039	It is shown that the heat treatment results in the formation of additional inter- and intramolecular bonds between carboxylic acids and polysaccharide molecules.
36496556	6	0	theme	mitochondrial	1378:1390	arg1	potential					1401:1409	mitochondrial membrane potential	1378:1409	mitochondrial membrane potential	1378:1409	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	5	1	theme	monosaccharide	1128:1141	arg1	composition					1143:1153	monosaccharide composition	1128:1153	monosaccharide composition	1128:1153	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	8	2	from	effects	2237:2243	arg1	colon					2252:2256	the colon	2248:2256	the colon	2248:2256	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	4	3	theme	performed	975:983	arg1	selenylation					994:1005	the performed chemical selenylation	971:1005	the performed chemical selenylation of PPS	971:1012	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	8	4	theme	inorganic	2091:2099	arg1	Se					2101:2102	inorganic Se	2091:2102	inorganic Se	2091:2102	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	7	5	theme	selenylation	1927:1938	arg1	degrees					1916:1922	higher degrees	1909:1922	higher degrees of selenylation	1909:1938	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	4	6	theme	higher	789:794	arg1	contents					796:803	higher contents	789:803	higher contents	789:803	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	3	7	theme	selenylated	535:545	arg1	products					547:554	two selenylated products	531:554	two selenylated products	531:554	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	3	7	theme	selenylated	535:545	arg1	SePPS2					576:581	SePPS2	576:581	SePPS2	576:581	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	3	7	theme	selenylated	535:545	arg1	SePPS1					565:570	SePPS1	565:570	SePPS1	565:570	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	8	8	theme	final	2113:2117	arg1	products					2123:2130	the final PPS products	2109:2130	the final PPS products	2109:2130	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	1	9	theme	research	210:217	arg1	focus					201:205	a focus	199:205	a focus of research	199:217	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	1	9	theme	research	210:217	arg1	effects					150:156	The anti-cancer effects	134:156	The anti-cancer effects of selenylated plant polysaccharides	134:193	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	2	10	with	plant	233:237	arg1	effects					265:271	extensive biological effects	244:271	extensive biological effects	244:271	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	4	11	theme	extracted	602:610	arg1	PPS					612:614	the extracted PPS	598:614	the extracted PPS	598:614	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	6	12	theme	morphology	1352:1361	arg1	alteration					1333:1342	the alteration	1329:1342	the alteration of cell morphology	1329:1361	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	12	theme	morphology	1352:1361	arg1	induction					1472:1480	the induction	1468:1480	the induction of cell apoptosis	1468:1498	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	12	theme	morphology	1352:1361	arg1	upregulation					1505:1516	upregulation	1505:1516	upregulation	1505:1516	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	12	theme	morphology	1352:1361	arg1	inhibition					1317:1326	cell growth inhibition	1305:1326	cell growth inhibition	1305:1326	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	12	theme	morphology	1352:1361	arg1	disruption					1364:1373	disruption	1364:1373	disruption of mitochondrial membrane potential	1364:1409	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	12	theme	morphology	1352:1361	arg1	downregulation					1521:1534	downregulation	1521:1534	downregulation	1521:1534	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	12	theme	morphology	1352:1361	arg1	generation					1456:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	4	13	from	fucose	766:771	arg1	fructose					856:863	fructose	856:863	fructose	856:863	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	13	from	fucose	766:771	arg1	acid					898:901	galacturonic acid	885:901	galacturonic acid	885:901	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	13	from	fucose	766:771	arg1	mannose					866:872	mannose	866:872	mannose	866:872	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	13	from	fucose	766:771	arg1	galactose					836:844	galactose	836:844	galactose	836:844	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	13	from	fucose	766:771	arg1	glucose					847:853	glucose	847:853	glucose	847:853	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	13	from	fucose	766:771	arg1	rhamnose					875:882	rhamnose	875:882	rhamnose	875:882	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	13	from	fucose	766:771	arg1	acid					919:922	glucuronic acid	908:922	glucuronic acid	908:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	13	from	fucose	766:771	arg1	saccharides					814:824	seven saccharides	808:824	seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid	808:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	fructose					856:863	fructose	856:863	fructose	856:863	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	acid					898:901	galacturonic acid	885:901	galacturonic acid	885:901	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	mannose					866:872	mannose	866:872	mannose	866:872	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	galactose					836:844	galactose	836:844	galactose	836:844	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	glucose					847:853	glucose	847:853	glucose	847:853	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	rhamnose					875:882	rhamnose	875:882	rhamnose	875:882	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	acid					919:922	glucuronic acid	908:922	glucuronic acid	908:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	14	from	saccharides-arabinose	743:763	arg1	saccharides					814:824	seven saccharides	808:824	seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid	808:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	8	15	theme	monosaccharide	2148:2161	arg1	composition					2163:2173	their monosaccharide composition	2142:2173	their monosaccharide composition	2142:2173	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	3	16	theme	HNO3-Na2SeO3	501:512	arg1	method					514:519	the HNO3-Na2SeO3 method	497:519	the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2	497:581	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	1	17	theme	anti-cancer	138:148	arg1	focus					201:205	a focus	199:205	a focus of research	199:217	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	1	17	theme	anti-cancer	138:148	arg1	effects					150:156	The anti-cancer effects	134:156	The anti-cancer effects of selenylated plant polysaccharides	134:193	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	7	18	from	effects	1799:1805	arg1	cells					1823:1827	the targeted cells	1810:1827	the targeted cells	1810:1827	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	6	19	theme	growth	1310:1315	arg1	inhibition					1317:1326	cell growth inhibition	1305:1326	cell growth inhibition	1305:1326	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	2	20	theme	extensive	244:252	arg1	effects					265:271	extensive biological effects	244:271	extensive biological effects	244:271	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	6	21	theme	cell	1485:1488	arg1	apoptosis					1490:1498	cell apoptosis	1485:1498	cell apoptosis	1485:1498	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	4	22	theme	glucuronic	908:917	arg1	acid					919:922	glucuronic acid	908:922	glucuronic acid	908:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	23	theme	higher	644:649	arg1	contents					654:661	much higher Se contents	639:661	much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg)	639:698	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	23	theme	higher	644:649	arg1	mg/kg					693:697	840.3 and 1770.5 versus 66.0 mg/kg	664:697	840.3 and 1770.5 versus 66.0 mg/kg	664:697	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	0	24	theme	Purslane	69:76	arg1	Polysaccharides					78:92	Purslane Polysaccharides	69:92	Purslane Polysaccharides	69:92	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	1	25	theme	plant	173:177	arg1	polysaccharides					179:193	selenylated plant polysaccharides	161:193	selenylated plant polysaccharides	161:193	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	6	26	theme	cell	1645:1648	arg1	apoptosis					1650:1658	cell apoptosis	1645:1658	cell apoptosis	1645:1658	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	5	27	theme	colon	1206:1210	arg1	cells					1227:1231	human colon cancer HCT-116 cells	1200:1231	human colon cancer HCT-116 cells	1200:1231	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	6	28	theme	proteins	1572:1579	arg1	PPS					1285:1287	PPS	1285:1287	PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway	1285:1724	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	4	29	theme	galacturonic	885:896	arg1	acid					898:901	galacturonic acid	885:901	galacturonic acid	885:901	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	0	30	theme	Covalent	102:109	arg1	Selenylation					120:131	a Covalent Chemical Selenylation	100:131	a Covalent Chemical Selenylation	100:131	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	7	31	theme	chemical	1860:1867	arg1	selenylation					1869:1880	the performed chemical selenylation	1846:1880	the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached)	1846:1951	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	6	32	theme	genes	1562:1566	arg1	PPS					1285:1287	PPS	1285:1287	PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway	1285:1724	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	4	33	theme	xylose	938:943	arg1	content					945:951	a stable xylose content	929:951	a stable xylose content	929:951	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	0	34	theme	Monosaccharide	0:13	arg1	Composition					15:25	Monosaccharide Composition	0:25	Monosaccharide Composition	0:25	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	5	35	theme	similar	1095:1101	arg1	features					1103:1110	similar features	1095:1110	similar features	1095:1110	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	6	36	theme	cell	1347:1350	arg1	morphology					1352:1361	cell morphology	1347:1361	cell morphology	1347:1361	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	3	37	theme	soluble	375:381	arg1	PPS					412:414	PPS	412:414	PPS	412:414	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	3	37	theme	soluble	375:381	arg1	polysaccharides					395:409	soluble P. oleracea polysaccharides	375:409	soluble P. oleracea polysaccharides (PPS)	375:415	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	6	38	theme	mitochondrial	1704:1716	arg1	pathway					1718:1724	the ROS-mediated mitochondrial pathway	1687:1724	the ROS-mediated mitochondrial pathway	1687:1724	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	8	39	theme	enhanced	2198:2205	arg1	effects					2237:2243	enhanced nutraceutical and anti-cancer effects	2198:2243	enhanced nutraceutical and anti-cancer effects in the colon	2198:2256	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	3	40	theme	P.	447:448	arg1	oleracea					450:457	the dried P. oleracea	437:457	the dried P. oleracea	437:457	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	4	41	theme	lower	719:723	arg1	contents					725:732	lower contents	719:732	lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid	719:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	6	42	theme	reactive	1426:1433	arg1	ROS					1451:1453	ROS	1451:1453	ROS	1451:1453	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	42	theme	reactive	1426:1433	arg1	species					1442:1448	intracellular reactive oxygen species	1412:1448	intracellular reactive oxygen species (ROS) generation	1412:1465	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	43	theme	cell	1305:1308	arg1	inhibition					1317:1326	cell growth inhibition	1305:1326	cell growth inhibition	1305:1326	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	44	theme	membrane	1392:1399	arg1	potential					1401:1409	mitochondrial membrane potential	1378:1409	mitochondrial membrane potential	1378:1409	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	8	45	theme	PPS	2063:2065	arg1	able					2071:2074	able	2071:2074	able	2071:2074	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	8	45	theme	PPS	2063:2065	arg1	selenylation					2047:2058	the performed selenylation	2033:2058	the performed selenylation of PPS	2033:2065	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	4	46	theme	chemical	985:992	arg1	selenylation					994:1005	the performed chemical selenylation	971:1005	the performed chemical selenylation of PPS	971:1012	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	47	contain	had	635:637	arg2	contents					654:661	much higher Se contents	639:661	much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg)	639:698	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	47	contain	had	635:637	arg1	SePPS1					617:622	SePPS1	617:622	SePPS1	617:622	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	47	contain	had	635:637	arg1	SePPS2					628:633	SePPS2	628:633	SePPS2	628:633	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	47	contain	had	635:637	arg2	mg/kg					693:697	840.3 and 1770.5 versus 66.0 mg/kg	664:697	840.3 and 1770.5 versus 66.0 mg/kg	664:697	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	2	48	theme	few	290:292	arg1	studies					294:300	few studies	290:300	few studies related to edible purslane (Portulaca oleracea L.)	290:351	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	5	49	contain	possessed	1159:1167	arg1	SePPS1					1070:1075	SePPS1	1070:1075	SePPS1	1070:1075	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	5	49	contain	possessed	1159:1167	arg2	bioactivity					1176:1186	higher bioactivity	1169:1186	higher bioactivity than PPS in human colon cancer HCT-116 cells	1169:1231	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	1	50	theme	polysaccharides	179:193	arg1	focus					201:205	a focus	199:205	a focus of research	199:217	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	1	50	theme	polysaccharides	179:193	arg1	effects					150:156	The anti-cancer effects	134:156	The anti-cancer effects of selenylated plant polysaccharides	134:193	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	2	51	theme	edible	313:318	arg1	Portulaca					330:338	Portulaca	330:338	Portulaca	330:338	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	2	51	theme	edible	313:318	arg1	purslane					320:327	edible purslane	313:327	edible purslane (Portulaca oleracea L.)	313:351	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	2	52	theme	related	302:308	arg1	studies					294:300	few studies	290:300	few studies related to edible purslane (Portulaca oleracea L.)	290:351	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	6	53	theme	cytochrome	1620:1629	arg1	C					1631:1631	cytochrome C	1620:1631	cytochrome C	1620:1631	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	4	54	theme	monosaccharide	1032:1045	arg1	composition					1047:1057	monosaccharide composition	1032:1057	monosaccharide composition	1032:1057	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	6	55	theme	species	1442:1448	arg1	generation					1456:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	4	56	from	changes	1021:1027	arg1	composition					1047:1057	monosaccharide composition	1032:1057	monosaccharide composition	1032:1057	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	7	57	theme	higher	1909:1914	arg1	degrees					1916:1922	higher degrees	1909:1922	higher degrees of selenylation	1909:1938	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	7	58	from	selenylation	1869:1880	arg1	particular					1893:1902	particular	1893:1902	particular	1893:1902	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	8	59	theme	PPS	2119:2121	arg1	products					2123:2130	the final PPS products	2109:2130	the final PPS products	2109:2130	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	5	60	from	bioactivity	1176:1186	arg1	cells					1227:1231	human colon cancer HCT-116 cells	1200:1231	human colon cancer HCT-116 cells	1200:1231	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	2	61	theme	natural	225:231	arg1	plant					233:237	a natural plant	223:237	a natural plant with extensive biological effects	223:271	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	8	62	theme	nutraceutical	2207:2219	arg1	effects					2237:2243	enhanced nutraceutical and anti-cancer effects	2198:2243	enhanced nutraceutical and anti-cancer effects in the colon	2198:2256	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	4	63	theme	Se	651:652	arg1	contents					654:661	much higher Se contents	639:661	much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg)	639:698	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	63	theme	Se	651:652	arg1	mg/kg					693:697	840.3 and 1770.5 versus 66.0 mg/kg	664:697	840.3 and 1770.5 versus 66.0 mg/kg	664:697	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	5	64	theme	cancer	1212:1217	arg1	cells					1227:1231	human colon cancer HCT-116 cells	1200:1231	human colon cancer HCT-116 cells	1200:1231	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	2	65	theme	biological	254:263	arg1	effects					265:271	extensive biological effects	244:271	extensive biological effects	244:271	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	6	66	theme	apoptosis	1490:1498	arg1	alteration					1333:1342	the alteration	1329:1342	the alteration of cell morphology	1329:1361	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	66	theme	apoptosis	1490:1498	arg1	induction					1472:1480	the induction	1468:1480	the induction of cell apoptosis	1468:1498	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	66	theme	apoptosis	1490:1498	arg1	upregulation					1505:1516	upregulation	1505:1516	upregulation	1505:1516	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	66	theme	apoptosis	1490:1498	arg1	inhibition					1317:1326	cell growth inhibition	1305:1326	cell growth inhibition	1305:1326	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	66	theme	apoptosis	1490:1498	arg1	disruption					1364:1373	disruption	1364:1373	disruption of mitochondrial membrane potential	1364:1409	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	66	theme	apoptosis	1490:1498	arg1	downregulation					1521:1534	downregulation	1521:1534	downregulation	1521:1534	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	66	theme	apoptosis	1490:1498	arg1	generation					1456:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	67	theme	growth	1664:1669	arg1	suppression					1671:1681	growth suppression	1664:1681	growth suppression	1664:1681	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	0	68	theme	Polysaccharides	78:92	arg1	Cells					60:64	HCT-116 Cells	52:64	HCT-116 Cells of Purslane Polysaccharides	52:92	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	7	69	theme	PPS	1885:1887	arg1	selenylation					1869:1880	the performed chemical selenylation	1846:1880	the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached)	1846:1951	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	1	70	theme	selenylated	161:171	arg1	polysaccharides					179:193	selenylated plant polysaccharides	161:193	selenylated plant polysaccharides	161:193	The anti-cancer effects of selenylated plant polysaccharides are a focus of research.
36496556	5	71	theme	human	1200:1204	arg1	cells					1227:1231	human colon cancer HCT-116 cells	1200:1231	human colon cancer HCT-116 cells	1200:1231	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	4	72	from	contents	725:732	arg1	ribose-and					778:787	ribose-and	778:787	ribose-and	778:787	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	72	from	contents	725:732	arg1	saccharides-arabinose					743:763	three saccharides-arabinose	737:763	three saccharides-arabinose	737:763	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	72	from	contents	725:732	arg1	fucose					766:771	fucose	766:771	fucose	766:771	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	0	73	theme	Chemical	111:118	arg1	Selenylation					120:131	a Covalent Chemical Selenylation	100:131	a Covalent Chemical Selenylation	100:131	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	4	74	from	ribose-and	778:787	arg1	fructose					856:863	fructose	856:863	fructose	856:863	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	74	from	ribose-and	778:787	arg1	acid					898:901	galacturonic acid	885:901	galacturonic acid	885:901	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	74	from	ribose-and	778:787	arg1	mannose					866:872	mannose	866:872	mannose	866:872	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	74	from	ribose-and	778:787	arg1	galactose					836:844	galactose	836:844	galactose	836:844	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	74	from	ribose-and	778:787	arg1	glucose					847:853	glucose	847:853	glucose	847:853	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	74	from	ribose-and	778:787	arg1	rhamnose					875:882	rhamnose	875:882	rhamnose	875:882	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	74	from	ribose-and	778:787	arg1	acid					919:922	glucuronic acid	908:922	glucuronic acid	908:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	4	74	from	ribose-and	778:787	arg1	saccharides					814:824	seven saccharides	808:824	seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid	808:922	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	7	75	theme	performed	1850:1858	arg1	selenylation					1869:1880	the performed chemical selenylation	1846:1880	the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached)	1846:1951	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	6	76	dep	inhibition	1317:1326	arg1	respect					1294:1300	respect	1294:1300	respect	1294:1300	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	77	theme	apoptosis-related	1544:1560	arg1	Bcl-2					1594:1598	Bcl-2	1594:1598	Bcl-2	1594:1598	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	77	theme	apoptosis-related	1544:1560	arg1	C					1631:1631	cytochrome C	1620:1631	cytochrome C	1620:1631	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	77	theme	apoptosis-related	1544:1560	arg1	genes					1562:1566	five apoptosis-related genes	1539:1566	five apoptosis-related genes	1539:1566	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	77	theme	apoptosis-related	1544:1560	arg1	Bax					1589:1591	Bax	1589:1591	Bax	1589:1591	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	77	theme	apoptosis-related	1544:1560	arg1	caspases-3/-9					1601:1613	caspases-3/-9	1601:1613	caspases-3/-9	1601:1613	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	4	78	theme	stable	931:936	arg1	content					945:951	a stable xylose content	929:951	a stable xylose content	929:951	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	3	79	dep	P.	383:384	arg1	oleracea					386:393	oleracea	386:393	oleracea	386:393	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	0	80	theme	In	31:32	arg1	Activity					40:47	In Vitro Activity	31:47	In Vitro Activity	31:47	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	8	81	theme	anti-cancer	2225:2235	arg1	effects					2237:2243	enhanced nutraceutical and anti-cancer effects	2198:2243	enhanced nutraceutical and anti-cancer effects in the colon	2198:2256	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	2	82	dep	Portulaca	330:338	arg1	oleracea					340:347	Portulaca oleracea L.	330:350	Portulaca oleracea L.	330:350	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	2	82	dep	Portulaca	330:338	arg1	L					349:349	L	349:349	Portulaca oleracea L.	330:350	As a natural plant with extensive biological effects, there have been few studies related to edible purslane (Portulaca oleracea L.).
36496556	8	83	dep	incorporate	2079:2089	arg1	endowing					2179:2186	endowing	2179:2186	endowing them with enhanced nutraceutical and anti-cancer effects in the colon	2179:2256	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	8	83	dep	incorporate	2079:2089	arg1	changing					2133:2140	changing	2133:2140	changing their monosaccharide composition	2133:2173	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	7	84	theme	targeted	1814:1821	arg1	cells					1823:1827	the targeted cells	1810:1827	the targeted cells	1810:1827	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	4	85	dep	saccharides-arabinose	743:763	arg1	contents					796:803	higher contents	789:803	higher contents	789:803	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	3	86	theme	P.	383:384	arg1	PPS					412:414	PPS	412:414	PPS	412:414	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	3	86	theme	P.	383:384	arg1	polysaccharides					395:409	soluble P. oleracea polysaccharides	375:409	soluble P. oleracea polysaccharides (PPS)	375:415	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	6	87	theme	ROS-mediated	1691:1702	arg1	pathway					1718:1724	the ROS-mediated mitochondrial pathway	1687:1724	the ROS-mediated mitochondrial pathway	1687:1724	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	0	88	theme	HCT-116	52:58	arg1	Cells					60:64	HCT-116 Cells	52:64	HCT-116 Cells of Purslane Polysaccharides	52:92	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	6	89	theme	oxygen	1435:1440	arg1	ROS					1451:1453	ROS	1451:1453	ROS	1451:1453	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	89	theme	oxygen	1435:1440	arg1	species					1442:1448	intracellular reactive oxygen species	1412:1448	intracellular reactive oxygen species (ROS) generation	1412:1465	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	7	90	theme	observed	1790:1797	arg1	effects					1799:1805	these observed effects	1784:1805	these observed effects on the targeted cells	1784:1827	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	7	91	from	increase	1968:1975	arg1	activity					1980:1987	activity	1980:1987	activity in the cells	1980:2000	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	0	92	dep	In	31:32	arg1	Vitro					34:38	Vitro	34:38	Vitro	34:38	Monosaccharide Composition and In Vitro Activity to HCT-116 Cells of Purslane Polysaccharides after a Covalent Chemical Selenylation.
36496556	8	93	theme	performed	2037:2045	arg1	able					2071:2074	able	2071:2074	able	2071:2074	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	8	93	theme	performed	2037:2045	arg1	selenylation					2047:2058	the performed selenylation	2033:2058	the performed selenylation of PPS	2033:2065	It can thus be concluded that the performed selenylation of PPS was able to incorporate inorganic Se into the final PPS products, changing their monosaccharide composition and endowing them with enhanced nutraceutical and anti-cancer effects in the colon.
36496556	3	94	theme	dried	441:445	arg1	oleracea					450:457	the dried P. oleracea	437:457	the dried P. oleracea	437:457	Thus, in this study, soluble P. oleracea polysaccharides (PPS) were extracted from the dried P. oleracea and then selenylated chemically using the HNO3-Na2SeO3 method to obtain two selenylated products, namely, SePPS1 and SePPS2.
36496556	6	95	theme	intracellular	1412:1424	arg1	ROS					1451:1453	ROS	1451:1453	ROS	1451:1453	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	95	theme	intracellular	1412:1424	arg1	species					1442:1448	intracellular reactive oxygen species	1412:1448	intracellular reactive oxygen species (ROS) generation	1412:1465	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	5	96	theme	higher	1169:1174	arg1	bioactivity					1176:1186	higher bioactivity	1169:1186	higher bioactivity than PPS in human colon cancer HCT-116 cells	1169:1231	Moreover, SePPS1 and SePPS2 shared similar features with respect to monosaccharide composition and possessed higher bioactivity than PPS in human colon cancer HCT-116 cells.
36496556	7	97	from	activity	1980:1987	arg1	cells					1996:2000	the cells	1992:2000	the cells	1992:2000	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	7	98	theme	highest	1758:1764	arg1	capacity					1766:1773	the highest capacity	1754:1773	the highest capacity to exert these observed effects on the targeted cells	1754:1827	SePPS2 consistently showed the highest capacity to exert these observed effects on the targeted cells, suggesting that the performed chemical selenylation of PPS (in particular when higher degrees of selenylation are reached) resulted in an increase in activity in the cells.
36496556	4	99	theme	PPS	1010:1012	arg1	selenylation					994:1005	the performed chemical selenylation	971:1005	the performed chemical selenylation of PPS	971:1012	Compared with the extracted PPS, SePPS1 and SePPS2 had much higher Se contents (840.3 and 1770.5 versus 66.0 mg/kg) while also showing lower contents in three saccharides-arabinose, fucose, and ribose-and higher contents in seven saccharides including galactose, glucose, fructose, mannose, rhamnose, galacturonic acid, and glucuronic acid, but a stable xylose content demonstrated that the performed chemical selenylation of PPS led to changes in monosaccharide composition.
36496556	6	100	theme	potential	1401:1409	arg1	alteration					1333:1342	the alteration	1329:1342	the alteration of cell morphology	1329:1361	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	100	theme	potential	1401:1409	arg1	induction					1472:1480	the induction	1468:1480	the induction of cell apoptosis	1468:1498	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	100	theme	potential	1401:1409	arg1	upregulation					1505:1516	upregulation	1505:1516	upregulation	1505:1516	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	100	theme	potential	1401:1409	arg1	inhibition					1317:1326	cell growth inhibition	1305:1326	cell growth inhibition	1305:1326	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	100	theme	potential	1401:1409	arg1	disruption					1364:1373	disruption	1364:1373	disruption of mitochondrial membrane potential	1364:1409	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	100	theme	potential	1401:1409	arg1	downregulation					1521:1534	downregulation	1521:1534	downregulation	1521:1534	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
36496556	6	100	theme	potential	1401:1409	arg1	generation					1456:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	intracellular reactive oxygen species (ROS) generation	1412:1465	Generally, SePPS1 and SePPS2 were more active than PPS with respect to cell growth inhibition, the alteration of cell morphology, disruption of mitochondrial membrane potential, intracellular reactive oxygen species (ROS) generation, the induction of cell apoptosis, and upregulation or downregulation of five apoptosis-related genes and proteins such as Bax, Bcl-2, caspases-3/-9, and cytochrome C, that cause cell apoptosis and growth suppression via the ROS-mediated mitochondrial pathway.
35718051	11	0	from	fibrosis	1886:1893	arg1	tissues					1917:1923	the colon and lung tissues	1898:1923	the colon and lung tissues	1898:1923	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	12	1	theme	plasma	2186:2191	arg1	levels					2197:2202	plasma LPS levels	2186:2202	plasma LPS levels	2186:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	2	from	restoration	2209:2219	arg1	colon					2166:2170	the colon	2162:2170	the colon	2162:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	2	from	restoration	2209:2219	arg1	levels					2197:2202	plasma LPS levels	2186:2202	plasma LPS levels	2186:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	13	3	theme	healthy	2647:2653	arg1	rats					2655:2658	healthy rats	2647:2658	healthy rats	2647:2658	MFE40 had no significant influence on the inflammatory factors in healthy rats.
35718051	1	4	theme	inflammation-related	261:280	arg1	diseases					282:289	other inflammation-related diseases	255:289	other inflammation-related diseases	255:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	9	5	theme	rRNA	1572:1575	arg1	sequencing					1582:1591	16S rRNA gene sequencing	1568:1591	16S rRNA gene sequencing	1568:1591	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	13	6	from	factors	2636:2642	arg1	rats					2655:2658	healthy rats	2647:2658	healthy rats	2647:2658	MFE40 had no significant influence on the inflammatory factors in healthy rats.
35718051	8	7	theme	IL-17	1180:1184	arg1	levels					1252:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels	1153:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma	1153:1292	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	12	8	from	colon	2166:2170	arg1	restoration					2209:2219	restoration	2209:2219	restoration	2209:2219	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	8	from	colon	2166:2170	arg1	expression					2127:2136	increased expression	2117:2136	increased expression of ZO-1 and occludin in the colon	2117:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	8	from	colon	2166:2170	arg1	reduction					2173:2181	reduction	2173:2181	reduction in plasma LPS levels	2173:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	7	9	theme	ultrahigh-performance	1018:1038	arg1	chromatography					1047:1060	ultrahigh-performance liquid chromatography	1018:1060	ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry	1018:1150	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	10	10	theme	ethanol	1651:1657	arg1	fraction					1659:1666	The 40% ethanol fraction	1643:1666	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters,	1635:1773	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	1	11	theme	traditional	173:183	arg1	Fructus					144:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus	108:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF)	108:155	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	11	theme	traditional	173:183	arg1	medicine					193:200	a well-known traditional Chinese medicine	160:200	a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases	160:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	8	12	theme	Interleukin	1153:1163	arg1	IL					1166:1167	Interleukin (IL)-1β	1153:1171	Interleukin (IL)-1β	1153:1171	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	11	13	from	inflammation	1869:1880	arg1	tissues					1917:1923	the colon and lung tissues	1898:1923	the colon and lung tissues	1898:1923	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	8	14	theme	transforming	1187:1198	arg1	factor					1207:1212	transforming growth factor	1187:1212	transforming growth factor (TGF)-β	1187:1220	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	8	14	theme	transforming	1187:1198	arg1	TGF					1215:1217	TGF	1215:1217	TGF	1215:1217	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	2	15	theme	anti-colitis	320:331	arg1	effect					333:338	the anti-colitis effect	316:338	the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	316:448	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	8	16	theme	IL	1166:1167	arg1	levels					1252:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels	1153:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma	1153:1292	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	9	17	theme	gas	1597:1599	arg1	spectrometry					1621:1632	gas chromatography/mass spectrometry	1597:1632	gas chromatography/mass spectrometry	1597:1632	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	14	18	theme	MFE40	2752:2756	arg1	esters					2709:2714	hydroxycinnamate esters	2692:2714	hydroxycinnamate esters	2692:2714	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	18	theme	MFE40	2752:2756	arg1	CONCLUSIONS					2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters	2661:2714	CONCLUSIONS	2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	18	theme	MFE40	2752:2756	arg1	constituents					2736:2747	the main active constituents	2720:2747	the main active constituents of MFE40	2720:2756	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	8	19	theme	factor	1207:1212	arg1	levels					1252:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels	1153:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma	1153:1292	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	4	20	dep	basis	572:576	arg1	the					559:561	the	559:561	the	559:561	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	5	21	theme	aqueous	782:788	arg1	solution					790:797	80% ethanol aqueous solution	770:797	80% ethanol aqueous solution	770:797	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	1	22	theme	Mume	139:142	arg1	Fructus					144:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus	108:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF)	108:155	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	22	theme	Mume	139:142	arg1	medicine					193:200	a well-known traditional Chinese medicine	160:200	a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases	160:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	22	theme	Mume	139:142	arg1	MF					153:154	MF	153:154	MF	153:154	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	12	23	theme	Clostridium_sensu_stricto_1	2288:2314	arg1	abundance					2260:2268	the relative abundance	2247:2268	the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum	2247:2450	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	7	24	theme	time-of-flight	1112:1125	arg1	spectrometry					1139:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	1	25	theme	chronic	216:222	arg1	cough					224:228	chronic cough	216:228	chronic cough	216:228	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	8	26	theme	IL-6	1174:1177	arg1	levels					1252:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels	1153:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma	1153:1292	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	14	27	theme	main	2724:2727	arg1	esters					2709:2714	hydroxycinnamate esters	2692:2714	hydroxycinnamate esters	2692:2714	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	27	theme	main	2724:2727	arg1	CONCLUSIONS					2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters	2661:2714	CONCLUSIONS	2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	27	theme	main	2724:2727	arg1	constituents					2736:2747	the main active constituents	2720:2747	the main active constituents of MFE40	2720:2756	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	8	28	theme	-β	1219:1220	arg1	factor					1207:1212	transforming growth factor	1187:1212	transforming growth factor (TGF)-β	1187:1220	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	8	28	theme	-β	1219:1220	arg1	TGF					1215:1217	TGF	1215:1217	TGF	1215:1217	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	12	29	from	reduction	2234:2242	arg1	abundance					2260:2268	the relative abundance	2247:2268	the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum	2247:2450	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	29	from	reduction	2234:2242	arg1	abundance					2481:2489	the relative abundance	2468:2489	the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus	2468:2578	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	5	30	theme	ethanol	834:840	arg1	solutions					850:858	0, 40, and 100% ethanol aqueous solutions	818:858	0, 40, and 100% ethanol aqueous solutions	818:858	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	12	31	theme	ZO-1	2141:2144	arg1	restoration					2209:2219	restoration	2209:2219	restoration	2209:2219	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	31	theme	ZO-1	2141:2144	arg1	expression					2127:2136	increased expression	2117:2136	increased expression of ZO-1 and occludin in the colon	2117:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	31	theme	ZO-1	2141:2144	arg1	reduction					2173:2181	reduction	2173:2181	reduction in plasma LPS levels	2173:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	32	theme	Butyricicoccus	2565:2578	arg1	abundance					2481:2489	the relative abundance	2468:2489	the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus	2468:2578	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	10	33	theme	ethyl	1722:1726	arg1	citrate					1728:1734	ethyl citrate	1722:1734	ethyl citrate	1722:1734	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	5	34	theme	aqueous	842:848	arg1	solutions					850:858	0, 40, and 100% ethanol aqueous solutions	818:858	0, 40, and 100% ethanol aqueous solutions	818:858	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	11	35	theme	CD	2059:2060	arg1	rats					2062:2065	CD rats	2059:2065	CD rats	2059:2065	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	12	36	theme	occludin	2150:2157	arg1	restoration					2209:2219	restoration	2209:2219	restoration	2209:2219	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	36	theme	occludin	2150:2157	arg1	expression					2127:2136	increased expression	2117:2136	increased expression of ZO-1 and occludin in the colon	2117:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	36	theme	occludin	2150:2157	arg1	reduction					2173:2181	reduction	2173:2181	reduction in plasma LPS levels	2173:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	14	37	theme	hydroxycinnamate	2692:2707	arg1	esters					2709:2714	hydroxycinnamate esters	2692:2714	hydroxycinnamate esters	2692:2714	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	37	theme	hydroxycinnamate	2692:2707	arg1	CONCLUSIONS					2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters	2661:2714	CONCLUSIONS	2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	37	theme	hydroxycinnamate	2692:2707	arg1	constituents					2736:2747	the main active constituents	2720:2747	the main active constituents of MFE40	2720:2756	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	12	38	from	expression	2127:2136	arg1	colon					2166:2170	the colon	2162:2170	the colon	2162:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	38	from	expression	2127:2136	arg1	levels					2197:2202	plasma LPS levels	2186:2202	plasma LPS levels	2186:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	5	39	theme	METHODS	740:746	arg1	MF					748:749	MATERIALS AND METHODS MF	726:749	MATERIALS AND METHODS MF	726:749	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	5	40	theme	MATERIALS	726:734	arg1	MF					748:749	MATERIALS AND METHODS MF	726:749	MATERIALS AND METHODS MF	726:749	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	10	41	from	CD	1834:1835	arg1	rats					1840:1843	rats	1840:1843	rats	1840:1843	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	2	42	from	effect	333:338	arg1	model					444:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	6	43	theme	CD	915:916	arg1	model					922:926	a TNBS-induced CD rat model	900:926	a TNBS-induced CD rat model	900:926	MFE and its fractions were screened in a TNBS-induced CD rat model.
35718051	10	44	dep	RESULTS	1635:1641	arg1	fraction					1659:1666	The 40% ethanol fraction	1643:1666	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters,	1635:1773	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	1	45	theme	ETHNOPHARMACOLOGICAL	108:127	arg1	Fructus					144:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus	108:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF)	108:155	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	45	theme	ETHNOPHARMACOLOGICAL	108:127	arg1	medicine					193:200	a well-known traditional Chinese medicine	160:200	a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases	160:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	45	theme	ETHNOPHARMACOLOGICAL	108:127	arg1	MF					153:154	MF	153:154	MF	153:154	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	9	46	theme	immunohistochemical	1447:1465	arg1	staining					1467:1474	immunohistochemical staining	1447:1474	immunohistochemical staining	1447:1474	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	8	47	link	enzyme-linked	1314:1326	arg1	assays					1342:1347	enzyme-linked immunosorbent assays	1314:1347	enzyme-linked immunosorbent assays	1314:1347	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	12	48	theme	Escherichia	2494:2504	arg1	shigella					2506:2513	Escherichia shigella	2494:2513	Escherichia shigella	2494:2513	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	5	49	dep	%	832:832	arg1	40					821:822	40	821:822	40	821:822	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	5	49	dep	%	832:832	arg1	100					829:831	100	829:831	100	829:831	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	12	50	theme	LPS	2193:2195	arg1	levels					2197:2202	plasma LPS levels	2186:2202	plasma LPS levels	2186:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	14	51	theme	Citrate	2673:2679	arg1	esters					2681:2686	Citrate esters	2673:2686	Citrate esters	2673:2686	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	2	52	theme	rat	440:442	arg1	model					444:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	4	53	theme	lung	686:689	arg1	injury					691:696	lung injury	686:696	lung injury	686:696	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	0	54	theme	MFE40-the	0:8	arg1	fraction					17:24	MFE40-the active fraction	0:24	MFE40-the active fraction of Mume Fructus alcohol	0:48	MFE40-the active fraction of Mume Fructus alcohol extract-alleviates Crohn's disease and its complications.
35718051	10	55	contain	contained	1696:1704	arg2	citrate					1728:1734	ethyl citrate	1722:1734	ethyl citrate	1722:1734	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	10	55	contain	contained	1696:1704	arg2	esters					1767:1772	caffeoylquinic acid ethyl esters	1741:1772	caffeoylquinic acid ethyl esters	1741:1772	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	10	55	contain	contained	1696:1704	arg1	MF					1671:1672	MF (MFE40)	1671:1680	MF (MFE40)	1671:1680	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	10	55	contain	contained	1696:1704	arg2	citrate					1713:1719	methyl citrate	1706:1719	methyl citrate	1706:1719	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	15	56	theme	lung	2846:2849	arg1	injury					2851:2856	lung injury	2846:2856	lung injury	2846:2856	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	2	57	theme	disease	427:433	arg1	model					444:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	5	58	theme	%	772:772	arg1	solution					790:797	80% ethanol aqueous solution	770:797	80% ethanol aqueous solution	770:797	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	9	59	theme	microbiota	1496:1505	arg1	levels					1541:1546	the intestinal microbiota and short-chain fatty acid (SCFA) levels	1481:1546	the intestinal microbiota and short-chain fatty acid (SCFA) levels	1481:1546	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	15	60	theme	homeostasis	2941:2951	arg1	regulation					2892:2901	regulation	2892:2901	regulation of the intestinal microbiota-gut-lung homeostasis	2892:2951	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	15	60	theme	homeostasis	2941:2951	arg1	effects					2880:2886	anti-inflammatory effects	2862:2886	anti-inflammatory effects	2862:2886	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	4	61	theme	STUDY	542:546	arg1	AIM					531:533	AIM	531:533	AIM OF THE STUDY	531:546	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	15	62	from	effect	2787:2792	arg1	rats					2800:2803	CD rats	2797:2803	CD rats	2797:2803	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	10	63	theme	caffeoylquinic	1741:1754	arg1	esters					1767:1772	caffeoylquinic acid ethyl esters	1741:1772	caffeoylquinic acid ethyl esters	1741:1772	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	7	64	theme	quadrupole	1101:1110	arg1	spectrometry					1139:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	0	65	theme	Mume	29:32	arg1	alcohol					42:48	Mume Fructus alcohol	29:48	Mume Fructus alcohol	29:48	MFE40-the active fraction of Mume Fructus alcohol extract-alleviates Crohn's disease and its complications.
35718051	9	66	theme	short-chain	1511:1521	arg1	SCFA					1535:1538	SCFA	1535:1538	SCFA	1535:1538	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	9	66	theme	short-chain	1511:1521	arg1	acid					1529:1532	short-chain fatty acid	1511:1532	short-chain fatty acid (SCFA)	1511:1539	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	15	67	theme	intestinal	2910:2919	arg1	homeostasis					2941:2951	the intestinal microbiota-gut-lung homeostasis	2906:2951	the intestinal microbiota-gut-lung homeostasis	2906:2951	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	11	68	theme	intestinal	2017:2026	arg1	obstruction					2028:2038	intestinal obstruction	2017:2038	intestinal obstruction	2017:2038	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	9	69	theme	expression	1354:1363	arg1	levels					1365:1370	The expression levels	1350:1370	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon	1350:1425	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	2	70	theme	extract	355:361	arg1	effect					333:338	the anti-colitis effect	316:338	the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	316:448	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	0	71	theme	alcohol	42:48	arg1	fraction					17:24	MFE40-the active fraction	0:24	MFE40-the active fraction of Mume Fructus alcohol	0:48	MFE40-the active fraction of Mume Fructus alcohol extract-alleviates Crohn's disease and its complications.
35718051	9	72	theme	acid	1529:1532	arg1	levels					1541:1546	the intestinal microbiota and short-chain fatty acid (SCFA) levels	1481:1546	the intestinal microbiota and short-chain fatty acid (SCFA) levels	1481:1546	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	8	73	theme	immunosorbent	1328:1340	arg1	assays					1342:1347	enzyme-linked immunosorbent assays	1314:1347	enzyme-linked immunosorbent assays	1314:1347	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	12	74	theme	Faecalibaculum	2341:2354	arg1	abundance					2260:2268	the relative abundance	2247:2268	the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum	2247:2450	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	9	75	from	levels	1365:1370	arg1	colon					1421:1425	the colon	1417:1425	the colon	1417:1425	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	12	76	from	reduction	2173:2181	arg1	colon					2166:2170	the colon	2162:2170	the colon	2162:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	76	from	reduction	2173:2181	arg1	levels					2197:2202	plasma LPS levels	2186:2202	plasma LPS levels	2186:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	10	77	theme	ethyl	1761:1765	arg1	esters					1767:1772	caffeoylquinic acid ethyl esters	1741:1772	caffeoylquinic acid ethyl esters	1741:1772	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	2	78	theme	sulfonic	390:397	arg1	acid					399:402	2,4,6-trinitrobenzene sulfonic acid	368:402	2,4,6-trinitrobenzene sulfonic acid	368:402	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	8	79	from	levels	1252:1257	arg1	lungs					1273:1277	lungs	1273:1277	lungs	1273:1277	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	8	79	from	levels	1252:1257	arg1	plasma					1287:1292	plasma	1287:1292	plasma	1287:1292	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	8	79	from	levels	1252:1257	arg1	colon					1266:1270	the colon	1262:1270	the colon	1262:1270	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	1	80	theme	prolonged	231:239	arg1	diarrhea					241:248	prolonged diarrhea	231:248	prolonged diarrhea	231:248	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	4	81	theme	ethanol	609:615	arg1	extract					617:623	the ethanol extract	605:623	the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction	605:723	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	12	82	theme	SCFAs	2224:2228	arg1	restoration					2209:2219	restoration	2209:2219	restoration	2209:2219	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	82	theme	SCFAs	2224:2228	arg1	expression					2127:2136	increased expression	2117:2136	increased expression of ZO-1 and occludin in the colon	2117:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	82	theme	SCFAs	2224:2228	arg1	reduction					2173:2181	reduction	2173:2181	reduction in plasma LPS levels	2173:2202	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	11	83	from	obstruction	2028:2038	arg1	rats					2062:2065	CD rats	2059:2065	CD rats	2059:2065	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	3	84	theme	underlying	487:496	arg1	mechanisms					498:507	underlying mechanisms	487:507	underlying mechanisms	487:507	However, the active ingredients and underlying mechanisms of MF remain unknown.
35718051	12	85	theme	Coriobacteriaceae_UGG_002	2409:2433	arg1	abundance					2260:2268	the relative abundance	2247:2268	the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum	2247:2450	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	7	86	theme	liquid	1040:1045	arg1	chromatography					1047:1060	ultrahigh-performance liquid chromatography	1018:1060	ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry	1018:1150	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	10	87	theme	MF	1671:1672	arg1	fraction					1659:1666	The 40% ethanol fraction	1643:1666	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters,	1635:1773	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	1	88	theme	other	255:259	arg1	diseases					282:289	other inflammation-related diseases	255:289	other inflammation-related diseases	255:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	9	89	theme	16S	1568:1570	arg1	sequencing					1582:1591	16S rRNA gene sequencing	1568:1591	16S rRNA gene sequencing	1568:1591	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	4	90	theme	material	563:570	arg1	basis					572:576	material basis	563:576	material basis	563:576	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	7	91	theme	tandem	1127:1132	arg1	spectrometry					1139:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	1	92	theme	well-known	162:171	arg1	Fructus					144:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus	108:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF)	108:155	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	92	theme	well-known	162:171	arg1	medicine					193:200	a well-known traditional Chinese medicine	160:200	a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases	160:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	12	93	from	ZO-1	2141:2144	arg1	colon					2166:2170	the colon	2162:2170	the colon	2162:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	9	94	theme	gene	1577:1580	arg1	sequencing					1582:1591	16S rRNA gene sequencing	1568:1591	16S rRNA gene sequencing	1568:1591	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	15	95	theme	intestinal	2819:2828	arg1	obstruction					2830:2840	intestinal obstruction	2819:2840	intestinal obstruction	2819:2840	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	4	96	theme	MF	628:629	arg1	extract					617:623	the ethanol extract	605:623	the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction	605:723	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	11	97	theme	lung	1912:1915	arg1	tissues					1917:1923	the colon and lung tissues	1898:1923	the colon and lung tissues	1898:1923	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	13	98	theme	inflammatory	2623:2634	arg1	factors					2636:2642	the inflammatory factors	2619:2642	the inflammatory factors in healthy rats	2619:2658	MFE40 had no significant influence on the inflammatory factors in healthy rats.
35718051	1	99	theme	Chinese	185:191	arg1	Fructus					144:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus	108:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF)	108:155	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	99	theme	Chinese	185:191	arg1	medicine					193:200	a well-known traditional Chinese medicine	160:200	a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases	160:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	15	100	theme	remission	2777:2785	arg1	effect					2787:2792	a remission effect	2775:2792	a remission effect on CD rats	2775:2803	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	11	101	theme	colon	1902:1906	arg1	tissues					1917:1923	the colon and lung tissues	1898:1923	the colon and lung tissues	1898:1923	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	5	102	theme	ethanol	774:780	arg1	solution					790:797	80% ethanol aqueous solution	770:797	80% ethanol aqueous solution	770:797	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	9	103	theme	chromatography/mass	1601:1619	arg1	spectrometry					1621:1632	gas chromatography/mass spectrometry	1597:1632	gas chromatography/mass spectrometry	1597:1632	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	15	104	theme	CD	2797:2798	arg1	rats					2800:2803	CD rats	2797:2803	CD rats	2797:2803	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	8	105	theme	growth	1200:1205	arg1	factor					1207:1212	transforming growth factor	1187:1212	transforming growth factor (TGF)-β	1187:1220	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	8	105	theme	growth	1200:1205	arg1	TGF					1215:1217	TGF	1215:1217	TGF	1215:1217	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	7	106	theme	chemical	961:968	arg1	composition					970:980	the chemical composition	957:980	the chemical composition	957:980	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	14	107	dep	CONCLUSIONS	2661:2671	arg1	esters					2681:2686	Citrate esters	2673:2686	Citrate esters	2673:2686	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	10	108	theme	methyl	1706:1711	arg1	citrate					1713:1719	methyl citrate	1706:1719	methyl citrate	1706:1719	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	13	109	theme	significant	2594:2604	arg1	influence					2606:2614	no significant influence	2591:2614	no significant influence	2591:2614	MFE40 had no significant influence on the inflammatory factors in healthy rats.
35718051	14	110	theme	active	2729:2734	arg1	esters					2709:2714	hydroxycinnamate esters	2692:2714	hydroxycinnamate esters	2692:2714	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	110	theme	active	2729:2734	arg1	CONCLUSIONS					2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters	2661:2714	CONCLUSIONS	2661:2671	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	14	110	theme	active	2729:2734	arg1	constituents					2736:2747	the main active constituents	2720:2747	the main active constituents of MFE40	2720:2756	CONCLUSIONS Citrate esters and hydroxycinnamate esters are the main active constituents of MFE40.
35718051	4	111	theme	extract	617:623	arg1	basis					572:576	material basis	563:576	material basis	563:576	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	4	111	theme	extract	617:623	arg1	mechanism					592:600	potential mechanism	582:600	potential mechanism	582:600	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	1	112	theme	RELEVANCE	129:137	arg1	Fructus					144:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus	108:150	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF)	108:155	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	112	theme	RELEVANCE	129:137	arg1	medicine					193:200	a well-known traditional Chinese medicine	160:200	a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases	160:289	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	1	112	theme	RELEVANCE	129:137	arg1	MF					153:154	MF	153:154	MF	153:154	ETHNOPHARMACOLOGICAL RELEVANCE Mume Fructus (MF) is a well-known traditional Chinese medicine used to treat chronic cough, prolonged diarrhea, and other inflammation-related diseases.
35718051	10	113	theme	40	1647:1648	arg1	%					1649:1649	%	1649:1649	%	1649:1649	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	7	114	theme	bioactive	937:945	arg1	fraction					947:954	the bioactive fraction	933:954	the bioactive fraction	933:954	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	9	115	theme	occludin	1405:1412	arg1	levels					1365:1370	The expression levels	1350:1370	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon	1350:1425	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	11	116	theme	cytokines	1956:1964	arg1	secretion					1943:1951	the secretion	1939:1951	the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β,	1939:2004	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	15	117	theme	anti-inflammatory	2862:2878	arg1	effects					2880:2886	anti-inflammatory effects	2862:2886	anti-inflammatory effects	2862:2886	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	9	118	theme	zonula	1375:1380	arg1	occludens-1					1382:1392	zonula occludens-1	1375:1392	zonula occludens-1 (ZO-1)	1375:1399	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	9	118	theme	zonula	1375:1380	arg1	ZO-1					1395:1398	ZO-1	1395:1398	ZO-1	1395:1398	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	11	119	dep	colon	1902:1906	arg1	the					1898:1900	the	1898:1900	the	1898:1900	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	12	120	theme	increased	2117:2125	arg1	expression					2127:2136	increased expression	2117:2136	increased expression of ZO-1 and occludin in the colon	2117:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	121	theme	Acetivibrio_ethanolgignens	2533:2558	arg1	abundance					2481:2489	the relative abundance	2468:2489	the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus	2468:2578	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	8	122	theme	lipopolysaccharide	1227:1244	arg1	levels					1252:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels	1153:1257	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma	1153:1292	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	6	123	theme	rat	918:920	arg1	model					922:926	a TNBS-induced CD rat model	900:926	a TNBS-induced CD rat model	900:926	MFE and its fractions were screened in a TNBS-induced CD rat model.
35718051	6	124	theme	TNBS-induced	902:913	arg1	model					922:926	a TNBS-induced CD rat model	900:926	a TNBS-induced CD rat model	900:926	MFE and its fractions were screened in a TNBS-induced CD rat model.
35718051	12	125	theme	MFE40	2095:2099	arg1	related					2106:2112	related	2106:2112	related	2106:2112	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	125	theme	MFE40	2095:2099	arg1	mechanisms					2081:2090	The possible mechanisms	2068:2090	The possible mechanisms of MFE40	2068:2099	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	4	126	theme	intestinal	702:711	arg1	obstruction					713:723	intestinal obstruction	702:723	intestinal obstruction	702:723	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	12	127	theme	shigella	2506:2513	arg1	abundance					2481:2489	the relative abundance	2468:2489	the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus	2468:2578	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	5	128	dep	solutions	850:858	arg1	%					832:832	%	832:832	%	832:832	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	3	129	theme	active	464:469	arg1	ingredients					471:481	active ingredients	464:481	active ingredients	464:481	However, the active ingredients and underlying mechanisms of MF remain unknown.
35718051	9	130	theme	occludens-1	1382:1392	arg1	levels					1365:1370	The expression levels	1350:1370	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon	1350:1425	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	3	131	dep	ingredients	471:481	arg1	the					460:462	the	460:462	the	460:462	However, the active ingredients and underlying mechanisms of MF remain unknown.
35718051	12	132	theme	possible	2072:2079	arg1	related					2106:2112	related	2106:2112	related	2106:2112	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	132	theme	possible	2072:2079	arg1	mechanisms					2081:2090	The possible mechanisms	2068:2090	The possible mechanisms of MFE40	2068:2099	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	0	133	theme	active	10:15	arg1	fraction					17:24	MFE40-the active fraction	0:24	MFE40-the active fraction of Mume Fructus alcohol	0:48	MFE40-the active fraction of Mume Fructus alcohol extract-alleviates Crohn's disease and its complications.
35718051	12	134	theme	relative	2251:2258	arg1	abundance					2260:2268	the relative abundance	2247:2268	the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum	2247:2450	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	135	theme	relative	2472:2479	arg1	abundance					2481:2489	the relative abundance	2468:2489	the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus	2468:2578	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	5	136	theme	80	770:771	arg1	%					772:772	%	772:772	%	772:772	MATERIALS AND METHODS MF was extracted with 80% ethanol aqueous solution and separated with 0, 40, and 100% ethanol aqueous solutions.
35718051	9	137	theme	intestinal	1485:1494	arg1	microbiota					1496:1505	intestinal microbiota	1485:1505	intestinal microbiota	1485:1505	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	15	138	theme	microbiota-gut-lung	2921:2939	arg1	homeostasis					2941:2951	the intestinal microbiota-gut-lung homeostasis	2906:2951	the intestinal microbiota-gut-lung homeostasis	2906:2951	MFE40 exhibited a remission effect on CD rats and inhibited intestinal obstruction and lung injury via anti-inflammatory effects and regulation of the intestinal microbiota-gut-lung homeostasis.
35718051	10	139	theme	acid	1756:1759	arg1	esters					1767:1772	caffeoylquinic acid ethyl esters	1741:1772	caffeoylquinic acid ethyl esters	1741:1772	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	10	140	theme	%	1649:1649	arg1	fraction					1659:1666	The 40% ethanol fraction	1643:1666	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters,	1635:1773	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	0	141	theme	Fructus	34:40	arg1	alcohol					42:48	Mume Fructus alcohol	29:48	Mume Fructus alcohol	29:48	MFE40-the active fraction of Mume Fructus alcohol extract-alleviates Crohn's disease and its complications.
35718051	11	142	theme	lung	2044:2047	arg1	injury					2049:2054	lung injury	2044:2054	lung injury	2044:2054	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	2	143	dep	-induced	410:417	arg1	acid					399:402	2,4,6-trinitrobenzene sulfonic acid	368:402	2,4,6-trinitrobenzene sulfonic acid	368:402	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	2	144	theme	-induced	410:417	arg1	model					444:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model	366:448	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	7	145	theme	diode-array	1075:1085	arg1	detection					1087:1095	diode-array detection	1075:1095	diode-array detection	1075:1095	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35718051	9	146	theme	fatty	1523:1527	arg1	SCFA					1535:1538	SCFA	1535:1538	SCFA	1535:1538	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	9	146	theme	fatty	1523:1527	arg1	acid					1529:1532	short-chain fatty acid	1511:1532	short-chain fatty acid (SCFA)	1511:1539	The expression levels of zonula occludens-1 (ZO-1) and occludin in the colon were measured using immunohistochemical staining, and the intestinal microbiota and short-chain fatty acid (SCFA) levels were analyzed using 16S rRNA gene sequencing and gas chromatography/mass spectrometry.
35718051	2	147	theme	2,4,6-trinitrobenzene	368:388	arg1	acid					399:402	2,4,6-trinitrobenzene sulfonic acid	368:402	2,4,6-trinitrobenzene sulfonic acid	368:402	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	11	148	from	injury	2049:2054	arg1	rats					2062:2065	CD rats	2059:2065	CD rats	2059:2065	MFE40 could ameliorate inflammation and fibrosis in the colon and lung tissues by inhibiting the secretion of cytokines, such as IL-1β, IL-6, IL-17, and TGF-β, along with intestinal obstruction and lung injury in CD rats.
35718051	2	149	theme	ethanol	347:353	arg1	extract					355:361	its ethanol extract	343:361	its ethanol extract	343:361	We previously confirmed the anti-colitis effect of its ethanol extract on a 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced Crohn's disease (CD) rat model.
35718051	4	150	theme	potential	582:590	arg1	mechanism					592:600	potential mechanism	582:600	potential mechanism	582:600	AIM OF THE STUDY To clarify the material basis and potential mechanism of the ethanol extract of MF (MFE) in alleviating CD and its complications, such as lung injury and intestinal obstruction.
35718051	13	151	contain	had	2587:2589	arg1	MFE40					2581:2585	MFE40	2581:2585	MFE40	2581:2585	MFE40 had no significant influence on the inflammatory factors in healthy rats.
35718051	13	151	contain	had	2587:2589	arg2	influence					2606:2614	no significant influence	2591:2614	no significant influence	2591:2614	MFE40 had no significant influence on the inflammatory factors in healthy rats.
35718051	8	152	theme	enzyme-linked	1314:1326	arg1	assays					1342:1347	enzyme-linked immunosorbent assays	1314:1347	enzyme-linked immunosorbent assays	1314:1347	Interleukin (IL)-1β, IL-6, IL-17, transforming growth factor (TGF)-β, and lipopolysaccharide (LPS) levels in the colon, lungs, and/or plasma were detected using enzyme-linked immunosorbent assays.
35718051	3	153	theme	MF	512:513	arg1	ingredients					471:481	active ingredients	464:481	active ingredients	464:481	However, the active ingredients and underlying mechanisms of MF remain unknown.
35718051	3	153	theme	MF	512:513	arg1	mechanisms					498:507	underlying mechanisms	487:507	underlying mechanisms	487:507	However, the active ingredients and underlying mechanisms of MF remain unknown.
35718051	12	154	from	occludin	2150:2157	arg1	colon					2166:2170	the colon	2162:2170	the colon	2162:2170	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	155	from	increase	2456:2463	arg1	abundance					2260:2268	the relative abundance	2247:2268	the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum	2247:2450	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	155	from	increase	2456:2463	arg1	abundance					2481:2489	the relative abundance	2468:2489	the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus	2468:2578	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	12	156	theme	Allobaculum	2440:2450	arg1	abundance					2260:2268	the relative abundance	2247:2268	the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum	2247:2450	The possible mechanisms of MFE40 were related to increased expression of ZO-1 and occludin in the colon, reduction in plasma LPS levels, and restoration of SCFAs via reduction in the relative abundance of Adlercreutzia, Clostridium_sensu_stricto_1, Erysipelatoclostridium, Faecalibaculum, norank_f_Erysipelotrichaceae, Phascolarctobacterium Coriobacteriaceae_UGG_002, and Allobaculum and increase in the relative abundance of Escherichia shigella, Christensenella, Acetivibrio_ethanolgignens, and Butyricicoccus.
35718051	10	157	theme	active	1797:1802	arg1	RESULTS					1635:1641	RESULTS	1635:1641	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters,	1635:1773	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	10	157	theme	active	1797:1802	arg1	fraction					1804:1811	the active fraction	1793:1811	the active fraction that could alleviate CD in rats	1793:1843	RESULTS The 40% ethanol fraction of MF (MFE40), which mainly contained methyl citrate, ethyl citrate, and caffeoylquinic acid ethyl esters, was identified as the active fraction that could alleviate CD in rats.
35718051	7	158	theme	mass	1134:1137	arg1	spectrometry					1139:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	quadrupole time-of-flight tandem mass spectrometry	1101:1150	For the bioactive fraction, the chemical composition was identified and quantified using ultrahigh-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight tandem mass spectrometry.
35651034	6	0	dep	stems	1092:1096	arg1	S50MW					1126:1130	S50MW	1126:1130	S50MW	1126:1130	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	7	1	theme	basic	1247:1251	arg1	chemistry					1253:1261	basic chemistry	1247:1261	basic chemistry	1247:1261	Wines were analyzed for basic chemistry, phenolic composition and color parameters, polysaccharides, and aroma profiles.
35651034	8	2	theme	lower	1380:1384	arg1	acidity					1395:1401	lower volatile acidity	1380:1401	lower volatile acidity	1380:1401	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	3	3	theme	aroma	460:464	arg1	contribution					466:477	phenolic and aroma contribution	447:477	phenolic and aroma contribution without additional cost	447:501	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	4	4	theme	combined	548:555	arg1	effect					557:562	the combined effect	544:562	the combined effect of MW application with stem additions	544:600	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	1	5	theme	low	239:241	arg1	wines					251:255	low quality wines	239:255	low quality wines	239:255	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	9	6	from	MW	1578:1579	arg1	season					1601:1606	the 2018 season	1592:1606	the 2018 season	1592:1606	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	9	7	theme	2018	1596:1599	arg1	season					1601:1606	the 2018 season	1592:1606	the 2018 season	1592:1606	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	0	8	theme	Microwave-assisted	0:17	arg1	maceration					19:28	Microwave-assisted maceration	0:28	Microwave-assisted maceration	0:28	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	6	9	dep	S50MW	1126:1130	arg1	60 °C					1151:1155	60 °C	1151:1155	60 °C	1151:1155	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	11	10	theme	polysaccharides	1916:1930	arg1	extraction					1932:1941	polysaccharides extraction	1916:1941	polysaccharides extraction	1916:1941	Likewise, combined strategies increased polysaccharides extraction and modified the volatile profile of wines.
35651034	12	11	theme	strategies	2115:2124	arg1	impact					2083:2088	the impact	2079:2088	the impact of the proposed combined strategies on the chemical composition of red wines	2079:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	4	12	theme	stem	587:590	arg1	additions					592:600	stem additions	587:600	stem additions	587:600	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	1	13	theme	high	185:188	arg1	yields					190:195	high yields	185:195	high yields per hectare generating, in several cases, low quality wines	185:255	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	12	14	theme	reported	1991:1998	arg1	results					2000:2006	The reported results	1987:2006	The reported results	1987:2006	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	12	14	theme	reported	1991:1998	arg1	promising					2012:2020	promising	2012:2020	promising	2012:2020	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	10	15	theme	marked	1811:1816	arg1	effect					1818:1823	a more marked effect	1804:1823	a more marked effect of MW on wine color (C*ab and polymeric pigments)	1804:1873	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	9	16	theme	polymeric	1655:1663	arg1	pigments					1665:1672	polymeric pigments	1655:1672	polymeric pigments	1655:1672	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	4	17	with	effect	557:562	arg1	additions					592:600	stem additions	587:600	stem additions	587:600	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	4	18	theme	Bonarda	679:685	arg1	wines					687:691	Bonarda wines	679:691	Bonarda wines	679:691	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	12	19	from	knowledge	2066:2074	arg1	composition					2142:2152	the chemical composition	2129:2152	the chemical composition of red wines	2129:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	1	20	from	variety	154:160	arg1	Argentina					165:173	Argentina	165:173	Argentina	165:173	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	1	21	theme	second	137:142	arg1	Bonarda					124:130	Bonarda	124:130	Bonarda	124:130	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	1	21	theme	second	137:142	arg1	variety					154:160	the second red grape variety	133:160	the second red grape variety in Argentina	133:173	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	6	22	theme	grape	929:933	arg1	[control					1033:1040	grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control	929:1040	grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control	929:1040	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	9	23	theme	tannin	1504:1509	arg1	content					1511:1517	tannin content	1504:1517	tannin content	1504:1517	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	10	24	theme	C	1846:1846	arg1	ab					1848:1849	C*ab	1846:1849	C*ab	1846:1849	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	6	25	theme	maceration	828:837	arg1	Factor					851:856	Factor 1	851:858	Factor 1	851:858	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	6	25	theme	maceration	828:837	arg1	strategies					839:848	Two maceration strategies	824:848	Two maceration strategies (Factor 1)	824:859	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	1	26	theme	grape	148:152	arg1	Bonarda					124:130	Bonarda	124:130	Bonarda	124:130	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	1	26	theme	grape	148:152	arg1	variety					154:160	the second red grape variety	133:160	the second red grape variety in Argentina	133:173	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	7	27	theme	aroma	1328:1332	arg1	profiles					1334:1341	aroma profiles	1328:1341	aroma profiles	1328:1341	Wines were analyzed for basic chemistry, phenolic composition and color parameters, polysaccharides, and aroma profiles.
35651034	11	28	theme	combined	1886:1893	arg1	strategies					1895:1904	combined strategies	1886:1904	combined strategies	1886:1904	Likewise, combined strategies increased polysaccharides extraction and modified the volatile profile of wines.
35651034	9	29	theme	greater	1707:1713	arg1	saturation					1715:1724	greater saturation	1707:1724	greater saturation	1707:1724	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	9	29	theme	greater	1707:1713	arg1	color					1700:1704	wine color	1695:1704	wine color (greater saturation)	1695:1725	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	0	30	theme	chemical	84:91	arg1	composition					93:103	wine chemical composition	79:103	wine chemical composition over two vintages	79:121	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	10	31	theme	polymeric	1855:1863	arg1	pigments					1865:1872	polymeric pigments	1855:1872	polymeric pigments	1855:1872	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	2	32	theme	other	359:363	arg1	foods					365:369	other foods	359:369	other foods	359:369	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	12	33	from	composition	2142:2152	arg1	knowledge					2066:2074	the knowledge	2062:2074	the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines	2062:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	0	34	from	addition	40:47	arg1	grapes					60:65	Bonarda grapes	52:65	Bonarda grapes	52:65	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	5	35	theme	experimental	747:758	arg1	design					760:765	the experimental design	743:765	the experimental design	743:765	During two consecutive vintages (2018 and 2019), the experimental design consisted of ten treatments (two factors) by triplicate.
35651034	1	36	theme	quality	243:249	arg1	wines					251:255	low quality wines	239:255	low quality wines	239:255	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	12	37	theme	chemical	2133:2140	arg1	composition					2142:2152	the chemical composition	2129:2152	the chemical composition of red wines	2129:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	8	38	dep	matrices	1450:1457	arg1	grapes					1460:1465	grapes	1460:1465	grapes	1460:1465	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	8	38	dep	matrices	1450:1457	arg1	stems					1471:1475	stems	1471:1475	stems	1471:1475	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	8	39	from	application	1430:1440	arg1	matrices					1450:1457	both matrices	1445:1457	both matrices (grapes and stems)	1445:1476	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	3	40	theme	Stems	372:376	arg1	technology					432:441	a sustainable technology	418:441	a sustainable technology for phenolic and aroma contribution without additional cost	418:501	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	3	40	theme	Stems	372:376	arg1	S					388:388	S	388:388	S	388:388	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	3	40	theme	Stems	372:376	arg1	addition					378:385	Stems addition	372:385	Stems addition (S) during vinification	372:409	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	12	41	theme	proposed	2097:2104	arg1	strategies					2115:2124	the proposed combined strategies	2093:2124	the proposed combined strategies	2093:2124	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	2	42	theme	extraction	304:313	arg1	extraction					277:286	Microwave-assisted extraction	258:286	Microwave-assisted extraction (MW)	258:291	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	2	42	theme	extraction	304:313	arg1	technique					315:323	a novel extraction technique	296:323	a novel extraction technique	296:323	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	0	43	theme	Bonarda	52:58	arg1	grapes					60:65	Bonarda grapes	52:65	Bonarda grapes	52:65	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	7	44	theme	phenolic	1264:1271	arg1	composition					1273:1283	phenolic composition	1264:1283	phenolic composition	1264:1283	Wines were analyzed for basic chemistry, phenolic composition and color parameters, polysaccharides, and aroma profiles.
35651034	9	45	theme	other	1554:1558	arg1	treatments					1560:1569	the other treatments	1550:1569	the other treatments	1550:1569	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	10	46	from	effect	1818:1823	arg1	color					1839:1843	wine color	1834:1843	wine color (C*ab and polymeric pigments)	1834:1873	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	3	47	theme	sustainable	420:430	arg1	technology					432:441	a sustainable technology	418:441	a sustainable technology for phenolic and aroma contribution without additional cost	418:501	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	3	47	theme	sustainable	420:430	arg1	addition					378:385	Stems addition	372:385	Stems addition (S) during vinification	372:409	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	9	48	theme	wine	1695:1698	arg1	saturation					1715:1724	greater saturation	1707:1724	greater saturation	1707:1724	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	9	48	theme	wine	1695:1698	arg1	color					1700:1704	wine color	1695:1704	wine color (greater saturation)	1695:1725	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	6	49	theme	stem-contact	998:1009	arg1	conditions					1011:1020	five stem-contact conditions	993:1020	five stem-contact conditions	993:1020	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	8	50	theme	higher	1366:1371	arg1	pH					1373:1374	higher pH	1366:1374	higher pH	1366:1374	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	6	51	theme	stems	1119:1123	arg1	addition + MW					1098:1110	addition + MW	1098:1110	addition + MW of the stems	1098:1123	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	8	52	theme	MW	1427:1428	arg1	application					1430:1440	MW application	1427:1440	MW application in both matrices (grapes and stems)	1427:1476	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	1	53	theme	several	224:230	arg1	cases					232:236	several cases	224:236	several cases	224:236	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	2	54	theme	Microwave-assisted	258:275	arg1	MW					289:290	MW	289:290	MW	289:290	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	2	54	theme	Microwave-assisted	258:275	arg1	technique					315:323	a novel extraction technique	296:323	a novel extraction technique	296:323	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	2	54	theme	Microwave-assisted	258:275	arg1	extraction					277:286	Microwave-assisted extraction	258:286	Microwave-assisted extraction (MW)	258:291	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	6	55	with	combined	979:986	arg1	conditions					1011:1020	five stem-contact conditions	993:1020	five stem-contact conditions	993:1020	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	12	56	theme	red	2157:2159	arg1	wines					2161:2165	red wines	2157:2165	red wines	2157:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	8	57	theme	volatile	1386:1393	arg1	acidity					1395:1401	lower volatile acidity	1380:1401	lower volatile acidity	1380:1401	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	3	58	theme	additional	487:496	arg1	cost					498:501	additional cost	487:501	additional cost	487:501	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	12	59	theme	wines	2161:2165	arg1	composition					2142:2152	the chemical composition	2129:2152	the chemical composition of red wines	2129:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	4	60	theme	different	605:613	arg1	conditions					615:624	different conditions	605:624	different conditions	605:624	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	10	61	theme	MW	1828:1829	arg1	effect					1818:1823	a more marked effect	1804:1823	a more marked effect of MW on wine color (C*ab and polymeric pigments)	1804:1873	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	9	62	theme	phenolic	1631:1638	arg1	extraction					1640:1649	phenolic extraction	1631:1649	phenolic extraction	1631:1649	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	11	63	theme	volatile	1960:1967	arg1	profile					1969:1975	the volatile profile	1956:1975	the volatile profile of wines	1956:1984	Likewise, combined strategies increased polysaccharides extraction and modified the volatile profile of wines.
35651034	2	64	theme	novel	298:302	arg1	extraction					277:286	Microwave-assisted extraction	258:286	Microwave-assisted extraction (MW)	258:291	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	2	64	theme	novel	298:302	arg1	technique					315:323	a novel extraction technique	296:323	a novel extraction technique	296:323	Microwave-assisted extraction (MW) is a novel extraction technique for winemaking, widely applied in other foods.
35651034	5	65	dep	vintages	717:724	arg1	2019					736:739	2019	736:739	2019	736:739	During two consecutive vintages (2018 and 2019), the experimental design consisted of ten treatments (two factors) by triplicate.
35651034	5	65	dep	vintages	717:724	arg1	2018					727:730	2018	727:730	2018	727:730	During two consecutive vintages (2018 and 2019), the experimental design consisted of ten treatments (two factors) by triplicate.
35651034	0	66	from	Effects	68:74	arg1	composition					93:103	wine chemical composition	79:103	wine chemical composition over two vintages	79:121	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	12	67	from	impact	2083:2088	arg1	composition					2142:2152	the chemical composition	2129:2152	the chemical composition of red wines	2129:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	6	68	dep	C	884:884	arg1	[control					874:881	[control	874:881	[control	874:881	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	10	69	theme	wine	1834:1837	arg1	color					1839:1843	wine color	1834:1843	wine color (C*ab and polymeric pigments)	1834:1873	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	4	70	theme	chemical	655:662	arg1	composition					664:674	the chemical composition	651:674	the chemical composition of Bonarda wines	651:691	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	10	71	with	similar	1790:1796	arg1	effect					1818:1823	a more marked effect	1804:1823	a more marked effect of MW on wine color (C*ab and polymeric pigments)	1804:1873	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	9	72	theme	Stem	1479:1482	arg1	additions					1484:1492	Stem additions	1479:1492	Stem additions	1479:1492	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	5	73	theme	consecutive	705:715	arg1	vintages					717:724	two consecutive vintages	701:724	two consecutive vintages (2018 and 2019)	701:740	During two consecutive vintages (2018 and 2019), the experimental design consisted of ten treatments (two factors) by triplicate.
35651034	12	74	dep	first	2045:2049	arg1	advance					2051:2057	advance	2051:2057	advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines	2051:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	9	75	dep	extraction	1640:1649	arg1	formation					1674:1682	formation	1674:1682	formation	1674:1682	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	6	76	theme	crushing	935:942	arg1	[control					1033:1040	grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control	929:1040	grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control	929:1040	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	10	77	theme	*	1847:1847	arg1	ab					1848:1849	C*ab	1846:1849	C*ab	1846:1849	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	0	78	theme	wine	79:82	arg1	composition					93:103	wine chemical composition	79:103	wine chemical composition over two vintages	79:121	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	1	79	theme	red	144:146	arg1	Bonarda					124:130	Bonarda	124:130	Bonarda	124:130	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	1	79	theme	red	144:146	arg1	variety					154:160	the second red grape variety	133:160	the second red grape variety in Argentina	133:173	Bonarda, the second red grape variety in Argentina, produces high yields per hectare generating, in several cases, low quality wines.
35651034	0	80	from	maceration	19:28	arg1	grapes					60:65	Bonarda grapes	52:65	Bonarda grapes	52:65	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	6	81	theme	microwaved-assisted	892:910	arg1	extraction					912:921	microwaved-assisted extraction	892:921	microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50%	892:1064	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	6	82	dep	crushing	935:942	arg1	combined					979:986	combined	979:986	combined	979:986	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	6	82	dep	crushing	935:942	arg1	MW					945:946	MW; 2450 MHz, 7600 W, 45-50 °C)	945:975	MW	945:946	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	8	83	from	additions	1413:1421	arg1	matrices					1450:1457	both matrices	1445:1457	both matrices (grapes and stems)	1445:1476	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	9	84	theme	violet	1744:1749	arg1	hue					1751:1753	violet hue	1744:1753	violet hue	1744:1753	Stem additions increased tannin content by >55% (S100) and by >25% for the other treatments; while MW, mostly in the 2018 season, consistently improved phenolic extraction and polymeric pigments formation, enhancing wine color (greater saturation) and intensifying violet hue.
35651034	4	85	theme	wines	687:691	arg1	composition					664:674	the chemical composition	651:674	the chemical composition of Bonarda wines	651:691	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	0	86	theme	stems	34:38	arg1	addition					40:47	stems addition	34:47	stems addition	34:47	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	12	87	theme	combined	2106:2113	arg1	strategies					2115:2124	the proposed combined strategies	2093:2124	the proposed combined strategies	2093:2124	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	10	88	dep	color	1839:1843	arg1	ab					1848:1849	C*ab	1846:1849	C*ab	1846:1849	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	10	88	dep	color	1839:1843	arg1	pigments					1865:1872	polymeric pigments	1855:1872	polymeric pigments	1855:1872	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	7	89	theme	color	1289:1293	arg1	parameters					1295:1304	color parameters	1289:1304	color parameters	1289:1304	Wines were analyzed for basic chemistry, phenolic composition and color parameters, polysaccharides, and aroma profiles.
35651034	6	90	theme	Factor	1023:1028	arg1	[control					1033:1040	grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control	929:1040	grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control	929:1040	Two maceration strategies (Factor 1) were applied [control (C), and microwaved-assisted extraction after grape crushing (MW; 2450 MHz, 7600 W, 45-50 °C)], combined with five stem-contact conditions (Factor 2) [control without stems (WS), 50% stems addition (S50), 50% stems addition + MW of the stems (S50MW; 2450 MHz, 7600 W, 60 °C), 100% stems addition (S100), 100% stems addition + MW (S100MW)].
35651034	12	91	theme	impact	2083:2088	arg1	knowledge					2066:2074	the knowledge	2062:2074	the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines	2062:2165	The reported results are promising and are considered the first advance in the knowledge of the impact of the proposed combined strategies on the chemical composition of red wines.
35651034	3	92	theme	phenolic	447:454	arg1	contribution					466:477	phenolic and aroma contribution	447:477	phenolic and aroma contribution without additional cost	447:501	Stems addition (S) during vinification can be a sustainable technology for phenolic and aroma contribution without additional cost.
35651034	8	93	theme	stem	1408:1411	arg1	additions					1413:1421	stem additions	1408:1421	stem additions	1408:1421	The 2018 wines showed higher pH and lower volatile acidity with stem additions and MW application in both matrices (grapes and stems).
35651034	10	94	located	observed	1769:1776	arg2	similar					1790:1796	similar	1790:1796	similar	1790:1796	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	10	94	located	observed	1769:1776	arg1	2019					1781:1784	2019	1781:1784	2019	1781:1784	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	10	94	located	observed	1769:1776	arg2	behavior					1760:1767	The behavior	1756:1767	The behavior observed in 2019	1756:1784	The behavior observed in 2019 was similar, with a more marked effect of MW on wine color (C*ab and polymeric pigments).
35651034	0	95	dep	maceration	19:28	arg1	Effects					68:74	Effects	68:74	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.	0:122	Microwave-assisted maceration and stems addition in Bonarda grapes: Effects on wine chemical composition over two vintages.
35651034	11	96	theme	wines	1980:1984	arg1	profile					1969:1975	the volatile profile	1956:1975	the volatile profile of wines	1956:1984	Likewise, combined strategies increased polysaccharides extraction and modified the volatile profile of wines.
35651034	4	97	theme	application	570:580	arg1	effect					557:562	the combined effect	544:562	the combined effect of MW application with stem additions	544:600	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
35651034	4	98	theme	MW	567:568	arg1	application					570:580	MW application	567:580	MW application	567:580	Therefore, this study aimed to evaluate the combined effect of MW application with stem additions in different conditions, before fermentation, on the chemical composition of Bonarda wines.
36840676	0	0	theme	Musts	86:90	arg1	Composition					56:66	Polysaccharide Composition	41:66	Polysaccharide Composition of White and Rosé Musts and Wines	41:100	Effect of Pre-fermentative Treatments on Polysaccharide Composition of White and Rosé Musts and Wines.
36840676	1	1	theme	pre-fermentative	149:164	arg1	techniques					166:175	conventional pre-fermentative techniques	136:175	conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM")	136:223	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	3	2	theme	polysaccharide	710:723	arg1	families					725:732	total soluble polysaccharide families	696:732	total soluble polysaccharide families	696:732	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	3	theme	white	366:370	arg1	wines					381:385	white and rosé wines	366:385	white and rosé wines	366:385	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	3	theme	white	366:370	arg1	musts					359:363	the musts	355:363	the musts	355:363	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	4	theme	techniques	166:175	arg1	effect					126:131	the effect	122:131	the effect	122:131	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	4	5	theme	wine	980:983	arg1	properties					995:1004	the wine colloidal properties	976:1004	the wine colloidal properties	976:1004	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	0	6	theme	Wines	96:100	arg1	Composition					56:66	Polysaccharide Composition	41:66	Polysaccharide Composition of White and Rosé Musts and Wines	41:100	Effect of Pre-fermentative Treatments on Polysaccharide Composition of White and Rosé Musts and Wines.
36840676	0	7	from	Effect	0:5	arg1	Composition					56:66	Polysaccharide Composition	41:66	Polysaccharide Composition of White and Rosé Musts and Wines	41:100	Effect of Pre-fermentative Treatments on Polysaccharide Composition of White and Rosé Musts and Wines.
36840676	2	8	theme	CM	511:512	arg1	technique					514:522	the CM technique	507:522	the CM technique	507:522	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	5	9	theme	pretreatments	1059:1071	arg1	effect					1049:1054	a clear effect	1041:1054	a clear effect of pretreatments on the evolution of polysaccharides	1041:1107	During bottle aging, there wasn't a clear effect of pretreatments on the evolution of polysaccharides.
36840676	1	10	theme	Viogner	292:298	arg1	grapes					315:320	Viogner and Monastrell grapes	292:320	Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging	292:423	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	11	theme	rosé	376:379	arg1	wines					381:385	white and rosé wines	366:385	white and rosé wines	366:385	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	11	theme	rosé	376:379	arg1	musts					359:363	the musts	355:363	the musts	355:363	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	3	12	theme	cell	816:819	arg1	polysaccharides					826:840	the grape berry cell wall polysaccharides	800:840	the grape berry cell wall polysaccharides	800:840	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	13	theme	innovate	232:239	arg1	technique					241:249	an innovate technique	229:249	an innovate technique (high power ultrasounds "S")	229:278	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	13	theme	innovate	232:239	arg1	"					277:277	high power ultrasounds "S"	252:277	high power ultrasounds "S"	252:277	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	3	14	theme	CP	641:642	arg1	treatment					644:652	CP treatment	641:652	CP treatment	641:652	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	2	15	theme	ultrasonic	546:555	arg1	maceration					557:566	the short ultrasonic maceration	536:566	the short ultrasonic maceration	536:566	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	16	dep	the	631:633	arg1	must					635:638	must	635:638	must	635:638	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	1	17	theme	Monastrell	304:313	arg1	grapes					315:320	Viogner and Monastrell grapes	292:320	Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging	292:423	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	2	18	theme	short	540:544	arg1	maceration					557:566	the short ultrasonic maceration	536:566	the short ultrasonic maceration	536:566	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	19	theme	polysaccharides	597:611	arg1	extraction					583:592	the extraction	579:592	the extraction of polysaccharides from the grape to the must	579:638	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	4	20	theme	polysaccharides	929:943	arg1	solubilization					911:924	the solubilization	907:924	the solubilization of polysaccharides in wines	907:952	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	3	21	theme	grape	804:808	arg1	polysaccharides					826:840	the grape berry cell wall polysaccharides	800:840	the grape berry cell wall polysaccharides	800:840	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	22	theme	direct	178:183	arg1	"					197:197	direct pressing "CP"	178:197	direct pressing "CP"	178:197	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	5	23	theme	polysaccharides	1093:1107	arg1	evolution					1080:1088	the evolution	1076:1088	the evolution of polysaccharides	1076:1107	During bottle aging, there wasn't a clear effect of pretreatments on the evolution of polysaccharides.
36840676	3	24	theme	berry	810:814	arg1	polysaccharides					826:840	the grape berry cell wall polysaccharides	800:840	the grape berry cell wall polysaccharides	800:840	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	25	theme	pressing	185:192	arg1	"					197:197	direct pressing "CP"	178:197	direct pressing "CP"	178:197	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	26	from	grapes	315:320	arg1	content					344:350	the polysaccharide content	325:350	the polysaccharide content of the musts, white and rosé wines,	325:386	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	27	theme	technique	241:249	arg1	effect					126:131	the effect	122:131	the effect	122:131	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	3	28	with	wines	663:667	arg1	content					685:691	the lowest content	674:691	the lowest content of total soluble polysaccharide families	674:732	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	29	dep	techniques	166:175	arg1	"					197:197	direct pressing "CP"	178:197	direct pressing "CP"	178:197	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	29	dep	techniques	166:175	arg1	"					222:222	cold maceration "CM"	203:222	cold maceration "CM"	203:222	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	2	30	from	grape	622:626	arg1	extraction					583:592	the extraction	579:592	the extraction of polysaccharides from the grape to the must	579:638	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	30	from	grape	622:626	arg1	polysaccharides					597:611	polysaccharides	597:611	polysaccharides from the grape to the must	597:638	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	0	31	theme	Treatments	27:36	arg1	Effect					0:5	Effect	0:5	Effect of Pre-fermentative Treatments on Polysaccharide Composition of White and Rosé Musts and Wines.	0:101	Effect of Pre-fermentative Treatments on Polysaccharide Composition of White and Rosé Musts and Wines.
36840676	3	32	theme	total	696:700	arg1	families					725:732	total soluble polysaccharide families	696:732	total soluble polysaccharide families	696:732	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	2	33	theme	pre-fermentation	461:476	arg1	key					572:574	key	572:574	key	572:574	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	33	theme	pre-fermentation	461:476	arg1	time					489:492	the longer pre-fermentation maceration time	450:492	the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration	450:566	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	1	34	theme	CP	195:196	arg1	"					197:197	direct pressing "CP"	178:197	direct pressing "CP"	178:197	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	0	35	theme	Pre-fermentative	10:25	arg1	Treatments					27:36	Pre-fermentative Treatments	10:36	Pre-fermentative Treatments	10:36	Effect of Pre-fermentative Treatments on Polysaccharide Composition of White and Rosé Musts and Wines.
36840676	1	36	theme	high	252:255	arg1	technique					241:249	an innovate technique	229:249	an innovate technique (high power ultrasounds "S")	229:278	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	36	theme	high	252:255	arg1	"					277:277	high power ultrasounds "S"	252:277	high power ultrasounds "S"	252:277	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	2	37	theme	longer	454:459	arg1	key					572:574	key	572:574	key	572:574	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	37	theme	longer	454:459	arg1	time					489:492	the longer pre-fermentation maceration time	450:492	the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration	450:566	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	3	38	theme	polysaccharides	826:840	arg1	disruption					786:795	the disruption	782:795	the disruption of the grape berry cell wall polysaccharides	782:840	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	0	39	theme	Polysaccharide	41:54	arg1	Composition					56:66	Polysaccharide Composition	41:66	Polysaccharide Composition of White and Rosé Musts and Wines	41:100	Effect of Pre-fermentative Treatments on Polysaccharide Composition of White and Rosé Musts and Wines.
36840676	1	40	theme	power	257:261	arg1	technique					241:249	an innovate technique	229:249	an innovate technique (high power ultrasounds "S")	229:278	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	40	theme	power	257:261	arg1	"					277:277	high power ultrasounds "S"	252:277	high power ultrasounds "S"	252:277	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	41	theme	ultrasounds	263:273	arg1	technique					241:249	an innovate technique	229:249	an innovate technique (high power ultrasounds "S")	229:278	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	41	theme	ultrasounds	263:273	arg1	"					277:277	high power ultrasounds "S"	252:277	high power ultrasounds "S"	252:277	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	4	42	theme	Ultrasonic	843:852	arg1	tool					890:893	a new tool	884:893	a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties	884:1004	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	4	42	theme	Ultrasonic	843:852	arg1	pretreatment					854:865	Ultrasonic pretreatment	843:865	Ultrasonic pretreatment	843:865	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	1	43	theme	polysaccharide	329:342	arg1	content					344:350	the polysaccharide content	325:350	the polysaccharide content of the musts, white and rosé wines,	325:386	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	3	44	theme	wall	821:824	arg1	polysaccharides					826:840	the grape berry cell wall polysaccharides	800:840	the grape berry cell wall polysaccharides	800:840	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	45	theme	cold	203:206	arg1	"					222:222	cold maceration "CM"	203:222	cold maceration "CM"	203:222	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	4	46	from	solubilization	911:924	arg1	wines					948:952	wines	948:952	wines	948:952	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	5	47	theme	bottle	1014:1019	arg1	aging					1021:1025	bottle aging	1014:1025	bottle aging	1014:1025	During bottle aging, there wasn't a clear effect of pretreatments on the evolution of polysaccharides.
36840676	5	48	theme	clear	1043:1047	arg1	effect					1049:1054	a clear effect	1041:1054	a clear effect of pretreatments on the evolution of polysaccharides	1041:1107	During bottle aging, there wasn't a clear effect of pretreatments on the evolution of polysaccharides.
36840676	1	49	theme	S	276:276	arg1	technique					241:249	an innovate technique	229:249	an innovate technique (high power ultrasounds "S")	229:278	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	49	theme	S	276:276	arg1	"					277:277	high power ultrasounds "S"	252:277	high power ultrasounds "S"	252:277	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	4	50	used	used	876:879	arg2	pretreatment					854:865	Ultrasonic pretreatment	843:865	Ultrasonic pretreatment	843:865	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	4	50	used	used	876:879	arg2	tool					890:893	a new tool	884:893	a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties	884:1004	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	1	51	theme	bottle	412:417	arg1	aging					419:423	bottle aging	412:423	bottle aging	412:423	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	5	52	from	effect	1049:1054	arg1	evolution					1080:1088	the evolution	1076:1088	the evolution of polysaccharides	1076:1107	During bottle aging, there wasn't a clear effect of pretreatments on the evolution of polysaccharides.
36840676	3	53	theme	lowest	678:683	arg1	content					685:691	the lowest content	674:691	the lowest content of total soluble polysaccharide families	674:732	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	54	theme	aging	419:423	arg1	months					402:407	six months	398:407	six months of bottle aging	398:423	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	4	55	theme	colloidal	985:993	arg1	properties					995:1004	the wine colloidal properties	976:1004	the wine colloidal properties	976:1004	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	1	56	theme	maceration	208:217	arg1	"					222:222	cold maceration "CM"	203:222	cold maceration "CM"	203:222	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	2	57	theme	maceration	478:487	arg1	key					572:574	key	572:574	key	572:574	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	57	theme	maceration	478:487	arg1	time					489:492	the longer pre-fermentation maceration time	450:492	the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration	450:566	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	1	58	theme	musts	359:363	arg1	content					344:350	the polysaccharide content	325:350	the polysaccharide content of the musts, white and rosé wines,	325:386	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	2	59	from	key	572:574	arg1	extraction					583:592	the extraction	579:592	the extraction of polysaccharides from the grape to the must	579:638	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	3	60	theme	families	725:732	arg1	content					685:691	the lowest content	674:691	the lowest content of total soluble polysaccharide families	674:732	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	4	61	theme	new	886:888	arg1	tool					890:893	a new tool	884:893	a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties	884:1004	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	4	61	theme	new	886:888	arg1	pretreatment					854:865	Ultrasonic pretreatment	843:865	Ultrasonic pretreatment	843:865	Ultrasonic pretreatment could be used as a new tool to increase the solubilization of polysaccharides in wines, positively affecting the wine colloidal properties.
36840676	2	62	from	extraction	583:592	arg1	grape					622:626	grape	622:626	grape	622:626	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	62	from	extraction	583:592	arg1	key					572:574	key	572:574	key	572:574	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	2	62	from	extraction	583:592	arg1	time					489:492	the longer pre-fermentation maceration time	450:492	the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration	450:566	The results showed that the longer pre-fermentation maceration time applied with the CM technique compared to the short ultrasonic maceration was key in the extraction of polysaccharides from the grape to the must.
36840676	3	63	theme	intense	757:763	arg1	pretreatment					765:776	the least intense pretreatment	747:776	the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides	747:840	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	3	63	theme	intense	757:763	arg1	it					740:741	it	740:741	it	740:741	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
36840676	1	64	theme	CM	220:221	arg1	"					222:222	cold maceration "CM"	203:222	cold maceration "CM"	203:222	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	1	65	theme	conventional	136:147	arg1	techniques					166:175	conventional pre-fermentative techniques	136:175	conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM")	136:223	This paper studied the effect of conventional pre-fermentative techniques (direct pressing "CP" and cold maceration "CM") and an innovate technique (high power ultrasounds "S"), applied to Viogner and Monastrell grapes on the polysaccharide content of the musts, white and rosé wines, and after six months of bottle aging.
36840676	3	66	theme	soluble	702:708	arg1	families					725:732	total soluble polysaccharide families	696:732	total soluble polysaccharide families	696:732	CP treatment produced wines with the lowest content of total soluble polysaccharide families since it was the least intense pretreatment for the disruption of the grape berry cell wall polysaccharides.
35799594	8	0	theme	basic	953:957	arg1	data					959:962	some useful basic data	941:962	some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries	941:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	0	1	from	Structure	20:28	arg1	Hull					91:94	Sesame Seed Hull	79:94	Sesame Seed Hull	79:94	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	8	2	from	polysaccharides	1013:1027	arg1	industries					1060:1069	the food and pharmaceutical industries	1032:1069	the food and pharmaceutical industries	1032:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	1	3	theme	seed	148:151	arg1	processing					153:162	sesame seed processing	141:162	sesame seed processing	141:162	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	0	4	theme	Seed	86:89	arg1	Hull					91:94	Sesame Seed Hull	79:94	Sesame Seed Hull	79:94	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	1	5	theme	processing	153:162	arg1	by-product					127:136	the major by-product	117:136	the major by-product of sesame seed processing	117:162	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	1	5	theme	processing	153:162	arg1	hull					109:112	Sesame seed hull	97:112	Sesame seed hull	97:112	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	7	6	theme	-linked	747:753	arg1	α-L-Xylp-					841:849	the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-	728:849	α-L-Xylp-	841:849	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	7	7	link	-linked	747:753	arg1	α-L-Xylp-					841:849	the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-	728:849	α-L-Xylp-	841:849	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	2	8	theme	%	328:328	arg1	yield					315:319	a yield	313:319	a yield of 6.49%	313:328	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	2	9	theme	ultrasound-assisted	262:280	arg1	methods					300:306	ultrasound-assisted alkali extraction methods	262:306	ultrasound-assisted alkali extraction methods	262:306	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	6	10	theme	galacturonic	599:610	arg1	acid					612:615	galacturonic acid	599:615	galacturonic acid (51.3%)	599:623	It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others.
35799594	6	10	theme	galacturonic	599:610	arg1	%					622:622	51.3%	618:622	51.3%	618:622	It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others.
35799594	5	11	with	SHP-2	551:555	arg1	yield					564:568	a yield	562:568	a yield of 3.78%	562:577	The dominant fraction was SHP-2 with a yield of 3.78%.
35799594	3	12	theme	purified	337:344	arg1	fractions					361:369	Three purified polysaccharide fractions	331:369	Three purified polysaccharide fractions	331:369	Three purified polysaccharide fractions were obtained after decolorization, deproteinization, and column chromatography.
35799594	7	13	dep	-linked	771:777	arg1	β-T-D-Rhap-linked					780:796	β-T-D-Rhap-linked	780:796	β-T-D-Rhap-linked	780:796	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	7	13	dep	-linked	771:777	arg1	-linked					813:819	-linked	813:819	-linked	813:819	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	7	13	dep	-linked	771:777	arg1	β-T-D-Galp-linked					822:838	β-T-D-Galp-linked	822:838	β-T-D-Galp-linked	822:838	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	7	14	theme	1,4	743:745	arg1	α-L-Xylp-					841:849	the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-	728:849	α-L-Xylp-	841:849	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	4	15	theme	main	464:467	arg1	composition					469:479	main composition	464:479	main composition	464:479	Then, their main composition and antioxidant activity were investigated.
35799594	3	16	theme	column	429:434	arg1	chromatography					436:449	column chromatography	429:449	column chromatography	429:449	Three purified polysaccharide fractions were obtained after decolorization, deproteinization, and column chromatography.
35799594	8	17	theme	developing	968:977	arg1	applications					979:990	developing applications	968:990	developing applications	968:990	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	0	18	from	Activity	46:53	arg1	Hull					91:94	Sesame Seed Hull	79:94	Sesame Seed Hull	79:94	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	7	19	theme	α-D-GalpA-	732:741	arg1	α-L-Xylp-					841:849	the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-	728:849	α-L-Xylp-	841:849	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	8	20	theme	sesame	996:1001	arg1	polysaccharides					1013:1027	sesame seed hull polysaccharides	996:1027	sesame seed hull polysaccharides in the food and pharmaceutical industries	996:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	8	21	theme	useful	946:951	arg1	data					959:962	some useful basic data	941:962	some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries	941:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	7	22	theme	linkage	700:706	arg1	types					708:712	The linkage types	696:712	The linkage types of SHP-2	696:721	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	8	23	theme	pharmaceutical	1045:1058	arg1	industries					1060:1069	the food and pharmaceutical industries	1032:1069	the food and pharmaceutical industries	1032:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	5	24	theme	%	577:577	arg1	yield					564:568	a yield	562:568	a yield of 3.78%	562:577	The dominant fraction was SHP-2 with a yield of 3.78%.
35799594	1	25	theme	Sesame	97:102	arg1	by-product					127:136	the major by-product	117:136	the major by-product of sesame seed processing	117:162	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	1	25	theme	Sesame	97:102	arg1	hull					109:112	Sesame seed hull	97:112	Sesame seed hull	97:112	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	3	26	theme	polysaccharide	346:359	arg1	fractions					361:369	Three purified polysaccharide fractions	331:369	Three purified polysaccharide fractions	331:369	Three purified polysaccharide fractions were obtained after decolorization, deproteinization, and column chromatography.
35799594	8	27	theme	hull	1008:1011	arg1	polysaccharides					1013:1027	sesame seed hull polysaccharides	996:1027	sesame seed hull polysaccharides in the food and pharmaceutical industries	996:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	2	28	theme	extraction	289:298	arg1	methods					300:306	ultrasound-assisted alkali extraction methods	262:306	ultrasound-assisted alkali extraction methods	262:306	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	0	29	theme	Structure	20:28	arg1	Characterization					0:15	Characterization	0:15	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.	0:95	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	0	30	from	Hull	91:94	arg1	Structure					20:28	Structure	20:28	Structure	20:28	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	0	30	from	Hull	91:94	arg1	Activity					46:53	Antioxidant Activity	34:53	Antioxidant Activity	34:53	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	0	30	from	Hull	91:94	arg1	Polysaccharides					58:72	Polysaccharides	58:72	Polysaccharides From Sesame Seed Hull	58:94	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	2	31	theme	alkali	282:287	arg1	methods					300:306	ultrasound-assisted alkali extraction methods	262:306	ultrasound-assisted alkali extraction methods	262:306	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	7	32	dep	α-L-Xylp-	841:849	arg1	1,4					851:853	1,4	851:853	1,4	851:853	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	0	33	theme	Activity	46:53	arg1	Characterization					0:15	Characterization	0:15	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.	0:95	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	2	34	theme	hull	217:220	arg1	SHP					239:241	SHP	239:241	SHP	239:241	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	2	34	theme	hull	217:220	arg1	polysaccharides					222:236	sesame hull polysaccharides	210:236	sesame hull polysaccharides (SHP)	210:242	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	7	35	theme	SHP-2	717:721	arg1	types					708:712	The linkage types	696:712	The linkage types of SHP-2	696:721	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	0	36	theme	Antioxidant	34:44	arg1	Activity					46:53	Antioxidant Activity	34:53	Antioxidant Activity	34:53	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	2	37	theme	sesame	210:215	arg1	SHP					239:241	SHP	239:241	SHP	239:241	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	2	37	theme	sesame	210:215	arg1	polysaccharides					222:236	sesame hull polysaccharides	210:236	sesame hull polysaccharides (SHP)	210:242	In this work, sesame hull polysaccharides (SHP) were extracted by ultrasound-assisted alkali extraction methods with a yield of 6.49%.
35799594	0	38	theme	Polysaccharides	58:72	arg1	Structure					20:28	Structure	20:28	Structure	20:28	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	0	38	theme	Polysaccharides	58:72	arg1	Activity					46:53	Antioxidant Activity	34:53	Antioxidant Activity	34:53	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	4	39	theme	antioxidant	485:495	arg1	activity					497:504	antioxidant activity	485:504	antioxidant activity	485:504	Then, their main composition and antioxidant activity were investigated.
35799594	1	40	from	polysaccharides	179:193	arg1	rich					171:174	rich	171:174	rich	171:174	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	6	41	theme	glucuronic	626:635	arg1	acid					637:640	glucuronic acid	626:640	glucuronic acid (13.8%)	626:648	It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others.
35799594	6	41	theme	glucuronic	626:635	arg1	%					647:647	13.8%	643:647	13.8%	643:647	It was composed of galacturonic acid (51.3%), glucuronic acid (13.8%), rhamnose (8.9%), glucose (8.4%), and others.
35799594	1	42	theme	major	121:125	arg1	by-product					127:136	the major by-product	117:136	the major by-product of sesame seed processing	117:162	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	1	42	theme	major	121:125	arg1	hull					109:112	Sesame seed hull	97:112	Sesame seed hull	97:112	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	1	43	theme	seed	104:107	arg1	by-product					127:136	the major by-product	117:136	the major by-product of sesame seed processing	117:162	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	1	43	theme	seed	104:107	arg1	hull					109:112	Sesame seed hull	97:112	Sesame seed hull	97:112	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	7	44	link	-linked	771:777	arg1	α-L-Xylp-					841:849	the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-	728:849	α-L-Xylp-	841:849	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	1	45	from	rich	171:174	arg1	polysaccharides					179:193	polysaccharides	179:193	polysaccharides	179:193	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35799594	8	46	theme	food	1036:1039	arg1	industries					1060:1069	the food and pharmaceutical industries	1032:1069	the food and pharmaceutical industries	1032:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	0	47	theme	Sesame	79:84	arg1	Hull					91:94	Sesame Seed Hull	79:94	Sesame Seed Hull	79:94	Characterization of Structure and Antioxidant Activity of Polysaccharides From Sesame Seed Hull.
35799594	5	48	theme	dominant	529:536	arg1	fraction					538:545	The dominant fraction	525:545	The dominant fraction	525:545	The dominant fraction was SHP-2 with a yield of 3.78%.
35799594	5	48	theme	dominant	529:536	arg1	SHP-2					551:555	SHP-2	551:555	SHP-2	551:555	The dominant fraction was SHP-2 with a yield of 3.78%.
35799594	7	49	theme	-linked	771:777	arg1	α-L-Xylp-					841:849	the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-	728:849	α-L-Xylp-	841:849	The linkage types of SHP-2 have the α-D-GalpA-(1,4)-linked, α-D-GlcpA-(1,2)-linked, β-T-D-Rhap-linked, β-D-Glcp-(1,6)-linked, β-T-D-Galp-linked, α-L-Xylp-(1,4)-linked, α-L-Araf-(1,3,5)-linked, and β-D-Manp-(1,4)-linked.
35799594	8	50	theme	seed	1003:1006	arg1	polysaccharides					1013:1027	sesame seed hull polysaccharides	996:1027	sesame seed hull polysaccharides in the food and pharmaceutical industries	996:1069	This study might provide some useful basic data for developing applications for sesame seed hull polysaccharides in the food and pharmaceutical industries.
35799594	1	51	theme	sesame	141:146	arg1	processing					153:162	sesame seed processing	141:162	sesame seed processing	141:162	Sesame seed hull is the major by-product of sesame seed processing and is rich in polysaccharides.
35308364	2	0	theme	Paraprevotella	481:494	arg1	abundance					436:444	the relative abundance	423:444	the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella	423:494	POP significantly upregulated the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella and significantly downregulated Escherichia_Shigella, Bacteroides, and Eubacterium nodatum groups.
35308364	1	1	theme	short-chain	322:332	arg1	acids					340:344	short-chain fatty acids	322:344	short-chain fatty acids in fermentation broth	322:366	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	4	2	from	conclusion	810:819	arg1	beneficial					829:838	beneficial	829:838	beneficial	829:838	In conclusion, POP is beneficial to aged rats because it can regulate intestinal flora, promote the growth of probiotics, and inhibit the reproduction of pathogenic bacteria.
35308364	3	3	theme	POP-treated	666:676	arg1	group					678:682	the POP-treated group	662:682	the POP-treated group	662:682	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	4	4	theme	pathogenic	961:970	arg1	bacteria					972:979	pathogenic bacteria	961:979	pathogenic bacteria	961:979	In conclusion, POP is beneficial to aged rats because it can regulate intestinal flora, promote the growth of probiotics, and inhibit the reproduction of pathogenic bacteria.
35308364	1	5	theme	fatty	334:338	arg1	acids					340:344	short-chain fatty acids	322:344	short-chain fatty acids in fermentation broth	322:366	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	4	6	from	beneficial	829:838	arg1	conclusion					810:819	conclusion	810:819	conclusion	810:819	In conclusion, POP is beneficial to aged rats because it can regulate intestinal flora, promote the growth of probiotics, and inhibit the reproduction of pathogenic bacteria.
35308364	1	7	theme	acids	340:344	arg1	level					313:317	the level	309:317	the level of short-chain fatty acids in fermentation broth	309:366	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	3	8	theme	acid-base	758:766	arg1	environment					768:778	the acid-base environment	754:778	the acid-base environment of the fermentation broth	754:804	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	4	9	theme	bacteria	972:979	arg1	reproduction					945:956	the reproduction	941:956	the reproduction of pathogenic bacteria	941:979	In conclusion, POP is beneficial to aged rats because it can regulate intestinal flora, promote the growth of probiotics, and inhibit the reproduction of pathogenic bacteria.
35308364	0	10	from	Microflora	55:64	arg1	Rats					74:77	Aged Rats	69:77	Aged Rats	69:77	Portulaca oleracea Polysaccharides Modulate Intestinal Microflora in Aged Rats in vitro.
35308364	0	11	theme	Portulaca	0:8	arg1	Polysaccharides					19:33	Portulaca oleracea Polysaccharides	0:33	Portulaca oleracea Polysaccharides	0:33	Portulaca oleracea Polysaccharides Modulate Intestinal Microflora in Aged Rats in vitro.
35308364	1	12	theme	intestinal	169:178	arg1	microflora					180:189	intestinal microflora	169:189	intestinal microflora in aged rats	169:202	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	0	13	theme	oleracea	10:17	arg1	Polysaccharides					19:33	Portulaca oleracea Polysaccharides	0:33	Portulaca oleracea Polysaccharides	0:33	Portulaca oleracea Polysaccharides Modulate Intestinal Microflora in Aged Rats in vitro.
35308364	4	14	theme	intestinal	877:886	arg1	flora					888:892	intestinal flora	877:892	intestinal flora	877:892	In conclusion, POP is beneficial to aged rats because it can regulate intestinal flora, promote the growth of probiotics, and inhibit the reproduction of pathogenic bacteria.
35308364	3	15	theme	fatty	717:721	arg1	acid					723:726	a more short-chain fatty acid	698:726	a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth	698:804	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	1	16	from	broth	362:366	arg1	level					313:317	the level	309:317	the level of short-chain fatty acids in fermentation broth	309:366	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	17	theme	16	267:268	arg1	S					270:270	S	270:270	S	270:270	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	3	18	theme	pH	599:600	arg1	value					602:606	The pH value	595:606	The pH value	595:606	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	1	19	from	acids	340:344	arg1	broth					362:366	fermentation broth	349:366	fermentation broth	349:366	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	20	theme	S	270:270	arg1	sequencing					293:302	16 S rDNA high-throughput sequencing	267:302	16 S rDNA high-throughput sequencing	267:302	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	2	21	dep	Escherichia_Shigella	528:547	arg1	groups					587:592	groups	587:592	groups	587:592	POP significantly upregulated the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella and significantly downregulated Escherichia_Shigella, Bacteroides, and Eubacterium nodatum groups.
35308364	4	22	theme	aged	843:846	arg1	rats					848:851	aged rats	843:851	aged rats	843:851	In conclusion, POP is beneficial to aged rats because it can regulate intestinal flora, promote the growth of probiotics, and inhibit the reproduction of pathogenic bacteria.
35308364	1	23	theme	aged	194:197	arg1	rats					199:202	aged rats	194:202	aged rats	194:202	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	24	theme	rDNA	272:275	arg1	sequencing					293:302	16 S rDNA high-throughput sequencing	267:302	16 S rDNA high-throughput sequencing	267:302	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	25	theme	high-throughput	277:291	arg1	sequencing					293:302	16 S rDNA high-throughput sequencing	267:302	16 S rDNA high-throughput sequencing	267:302	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	3	26	theme	ammonia	612:618	arg1	level					629:633	ammonia nitrogen level	612:633	ammonia nitrogen level	612:633	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	0	27	theme	Intestinal	44:53	arg1	Microflora					55:64	Intestinal Microflora	44:64	Intestinal Microflora in Aged Rats	44:77	Portulaca oleracea Polysaccharides Modulate Intestinal Microflora in Aged Rats in vitro.
35308364	2	28	theme	Eggerthella	464:474	arg1	abundance					436:444	the relative abundance	423:444	the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella	423:494	POP significantly upregulated the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella and significantly downregulated Escherichia_Shigella, Bacteroides, and Eubacterium nodatum groups.
35308364	2	29	theme	relative	427:434	arg1	abundance					436:444	the relative abundance	423:444	the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella	423:494	POP significantly upregulated the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella and significantly downregulated Escherichia_Shigella, Bacteroides, and Eubacterium nodatum groups.
35308364	0	30	theme	Aged	69:72	arg1	Rats					74:77	Aged Rats	69:77	Aged Rats	69:77	Portulaca oleracea Polysaccharides Modulate Intestinal Microflora in Aged Rats in vitro.
35308364	3	31	theme	fermentation	787:798	arg1	broth					800:804	the fermentation broth	783:804	the fermentation broth	783:804	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	1	32	theme	fermentation	349:360	arg1	broth					362:366	fermentation broth	349:366	fermentation broth	349:366	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	2	33	theme	Lactobacillus	449:461	arg1	abundance					436:444	the relative abundance	423:444	the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella	423:494	POP significantly upregulated the relative abundance of Lactobacillus, Eggerthella, and Paraprevotella and significantly downregulated Escherichia_Shigella, Bacteroides, and Eubacterium nodatum groups.
35308364	3	34	theme	broth	800:804	arg1	environment					768:778	the acid-base environment	754:778	the acid-base environment of the fermentation broth	754:804	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	1	35	theme	Portulaca	114:122	arg1	POP					150:152	POP	150:152	POP	150:152	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	35	theme	Portulaca	114:122	arg1	polysaccharides					133:147	Portulaca oleracea polysaccharides	114:147	Portulaca oleracea polysaccharides (POP)	114:153	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	3	36	theme	short-chain	705:715	arg1	acid					723:726	a more short-chain fatty acid	698:726	a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth	698:804	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	3	37	theme	nitrogen	620:627	arg1	level					629:633	ammonia nitrogen level	612:633	ammonia nitrogen level	612:633	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	1	38	theme	oleracea	124:131	arg1	POP					150:152	POP	150:152	POP	150:152	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	38	theme	oleracea	124:131	arg1	polysaccharides					133:147	Portulaca oleracea polysaccharides	114:147	Portulaca oleracea polysaccharides (POP)	114:153	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	4	39	theme	probiotics	917:926	arg1	growth					907:912	the growth	903:912	the growth of probiotics	903:926	In conclusion, POP is beneficial to aged rats because it can regulate intestinal flora, promote the growth of probiotics, and inhibit the reproduction of pathogenic bacteria.
35308364	1	40	from	microflora	180:189	arg1	rats					199:202	aged rats	194:202	aged rats	194:202	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	41	theme	polysaccharides	133:147	arg1	effect					104:109	the effect	100:109	the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro	100:211	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	42	theme	intestinal	218:227	arg1	composition					239:249	its intestinal microbial composition	214:249	its intestinal microbial composition	214:249	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	1	43	from	level	313:317	arg1	broth					362:366	fermentation broth	349:366	fermentation broth	349:366	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
35308364	3	44	theme	acid	723:726	arg1	consumption					728:738	a more short-chain fatty acid consumption	698:738	a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth	698:804	The pH value and ammonia nitrogen level decreased significantly in the POP-treated group, resulting in a more short-chain fatty acid consumption which changed the acid-base environment of the fermentation broth.
35308364	1	45	theme	microbial	229:237	arg1	composition					239:249	its intestinal microbial composition	214:249	its intestinal microbial composition	214:249	To explore the effect of Portulaca oleracea polysaccharides (POP) in regulating intestinal microflora in aged rats in vitro, its intestinal microbial composition was analyzed by 16 S rDNA high-throughput sequencing, and the level of short-chain fatty acids in fermentation broth was determined by LC-MS.
36365633	0	0	theme	Cordyceps	98:106	arg1	militaris					108:116	Cordyceps militaris	98:116	Cordyceps militaris	98:116	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	5	1	theme	C5	1044:1045	arg1	components					1078:1087	C2, C5, C9, factor B, factor B, and P components	1040:1087	C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system	1040:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	5	2	theme	overactivation	1096:1109	arg1	cascade					1111:1117	the overactivation cascade	1092:1117	the overactivation cascade of the complement system	1092:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	2	3	theme	cellulose	380:388	arg1	column					390:395	a DEAE-52 cellulose column	370:395	a DEAE-52 cellulose column	370:395	The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column.
36365633	4	4	theme	classical	918:926	arg1	pathway					928:934	the classical pathway	914:934	the classical pathway	914:934	The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway.
36365633	3	5	contain	had	737:739	arg2	conformation					756:767	a triple-helix conformation	741:767	a triple-helix conformation	741:767	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	5	contain	had	737:739	arg1	all					733:735	all	733:735	all	733:735	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	6	theme	homogenous	590:599	arg1	polysaccharides					569:583	the three polysaccharides	559:583	the three polysaccharides	559:583	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	6	theme	homogenous	590:599	arg1	polysaccharides					601:615	homogenous polysaccharides	590:615	homogenous polysaccharides with different molecular weights	590:648	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	5	7	theme	P	1076:1076	arg1	components					1078:1087	C2, C5, C9, factor B, factor B, and P components	1040:1087	C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system	1040:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	5	8	theme	C9	1048:1049	arg1	components					1078:1087	C2, C5, C9, factor B, factor B, and P components	1040:1087	C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system	1040:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	6	9	theme	polysaccharides	1341:1355	arg1	activity					1329:1336	the anti-complementary activity	1306:1336	the anti-complementary activity of polysaccharides	1306:1355	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	3	10	with	monosaccharides	684:698	arg1	ratios					721:726	different molar ratios	705:726	different molar ratios	705:726	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	5	11	theme	mechanism	973:981	arg1	studies					983:989	Preliminary mechanism studies	961:989	Preliminary mechanism studies	961:989	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	3	12	theme	triple-helix	743:754	arg1	conformation					756:767	a triple-helix conformation	741:767	a triple-helix conformation	741:767	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	13	used	used	449:452	arg2	HPLC					402:405	The HPLC	398:405	The HPLC	398:405	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	13	used	used	449:452	arg2	analyses					435:442	Congo red analyses	425:442	Congo red analyses	425:442	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	13	used	used	449:452	arg2	FT-IR					415:419	FT-IR	415:419	FT-IR	415:419	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	13	used	used	449:452	arg2	HPGPC					408:412	HPGPC	408:412	HPGPC	408:412	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	5	14	theme	Preliminary	961:971	arg1	studies					983:989	Preliminary mechanism studies	961:989	Preliminary mechanism studies	961:989	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	6	15	theme	anti-complementary	1310:1327	arg1	activity					1329:1336	the anti-complementary activity	1306:1336	the anti-complementary activity of polysaccharides	1306:1355	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	2	16	dep	militaris	315:323	arg1	CMP-3					343:347	CMP-3	343:347	CMP-3	343:347	The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column.
36365633	2	16	dep	militaris	315:323	arg1	CMP-2					333:337	CMP-2	333:337	CMP-2	333:337	The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column.
36365633	2	16	dep	militaris	315:323	arg1	CMP-1					326:330	CMP-1	326:330	CMP-1	326:330	The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column.
36365633	6	17	theme	galacturonic	1244:1255	arg1	composition					1262:1272	galacturonic acid composition	1244:1272	galacturonic acid composition	1244:1272	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	3	18	theme	molar	715:719	arg1	ratios					721:726	different molar ratios	705:726	different molar ratios	705:726	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	5	19	theme	C2	1040:1041	arg1	components					1078:1087	C2, C5, C9, factor B, factor B, and P components	1040:1087	C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system	1040:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	3	20	with	polysaccharides	601:615	arg1	weights					642:648	different molecular weights	622:648	different molecular weights	622:648	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	5	21	theme	factor	1062:1067	arg1	B					1069:1069	factor B	1062:1069	factor B	1062:1069	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	4	22	theme	activity	807:814	arg1	evaluation					774:783	The evaluation	770:783	The evaluation of anti-complementary activity	770:814	The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway.
36365633	5	23	from	components	1078:1087	arg1	cascade					1111:1117	the overactivation cascade	1092:1117	the overactivation cascade of the complement system	1092:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	1	24	theme	Cordyceps	186:194	arg1	militaris					196:204	Cordyceps militaris	186:204	Cordyceps militaris	186:204	This investigation focuses on the three novel polysaccharides from Cordyceps militaris and then discusses their characterization and anti-complementary activity.
36365633	2	25	from	militaris	315:323	arg1	polysaccharides					291:305	The three polysaccharides	281:305	The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3)	281:348	The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column.
36365633	6	26	theme	correlation	1173:1183	arg1	analysis					1149:1156	The analysis	1145:1156	The analysis of the Pearson correlation and network	1145:1195	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	0	27	theme	Anti-Complementary	34:51	arg1	Activity					53:60	Anti-Complementary Activity	34:60	Anti-Complementary Activity	34:60	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	3	28	theme	red	431:433	arg1	analyses					435:442	Congo red analyses	425:442	Congo red analyses	425:442	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	5	29	theme	complement	1126:1135	arg1	system					1137:1142	the complement system	1122:1142	the complement system	1122:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	1	30	theme	anti-complementary	252:269	arg1	activity					271:278	anti-complementary activity	252:278	anti-complementary activity	252:278	This investigation focuses on the three novel polysaccharides from Cordyceps militaris and then discusses their characterization and anti-complementary activity.
36365633	5	31	theme	system	1137:1142	arg1	cascade					1111:1117	the overactivation cascade	1092:1117	the overactivation cascade of the complement system	1092:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	3	32	theme	different	622:630	arg1	weights					642:648	different molecular weights	622:648	different molecular weights	622:648	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	4	33	theme	alternative	940:950	arg1	pathway					952:958	alternative pathway	940:958	alternative pathway	940:958	The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway.
36365633	2	34	theme	DEAE-52	372:378	arg1	column					390:395	a DEAE-52 cellulose column	370:395	a DEAE-52 cellulose column	370:395	The three polysaccharides from C. militaris (CMP-1, CMP-2 and CMP-3) were prepared using a DEAE-52 cellulose column.
36365633	6	35	theme	glucuronic	1224:1233	arg1	acid					1235:1238	glucuronic acid	1224:1238	glucuronic acid	1224:1238	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	3	36	theme	molecular	632:640	arg1	weights					642:648	different molecular weights	622:648	different molecular weights	622:648	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	37	theme	stereo	514:519	arg1	monosaccharides					476:490	their monosaccharides	470:490	their monosaccharides	470:490	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	37	theme	stereo	514:519	arg1	conformation					521:532	stereo conformation	514:532	stereo conformation	514:532	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	7	38	theme	anti-complementary	1448:1465	arg1	drugs					1467:1471	anti-complementary drugs	1448:1471	anti-complementary drugs	1448:1471	These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.
36365633	5	39	theme	B	1059:1059	arg1	components					1078:1087	C2, C5, C9, factor B, factor B, and P components	1040:1087	C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system	1040:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	3	40	theme	molecular	493:501	arg1	monosaccharides					476:490	their monosaccharides	470:490	their monosaccharides	470:490	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	3	40	theme	molecular	493:501	arg1	weight					503:508	molecular weight	493:508	molecular weight	493:508	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	5	41	theme	factor	1052:1057	arg1	B					1059:1059	factor B	1052:1059	factor B	1052:1059	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	3	42	theme	different	705:713	arg1	ratios					721:726	different molar ratios	705:726	different molar ratios	705:726	The HPLC, HPGPC, FT-IR and Congo red analyses were used to characterize their monosaccharides, molecular weight and stereo conformation, which demonstrated that the three polysaccharides were homogenous polysaccharides with different molecular weights and were composed of at least ten monosaccharides with different molar ratios, and all had a triple-helix conformation.
36365633	6	43	theme	acid	1257:1260	arg1	composition					1262:1272	galacturonic acid composition	1244:1272	galacturonic acid composition	1244:1272	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	4	44	theme	anti-complementary	788:805	arg1	activity					807:814	anti-complementary activity	788:814	anti-complementary activity	788:814	The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway.
36365633	7	45	theme	novel	1397:1401	arg1	polysaccharides					1403:1417	the three novel polysaccharides	1387:1417	the three novel polysaccharides	1387:1417	These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.
36365633	7	45	theme	novel	1397:1401	arg1	candidates					1433:1442	potential candidates	1423:1442	potential candidates for anti-complementary drugs	1423:1471	These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.
36365633	6	46	theme	Pearson	1165:1171	arg1	correlation					1173:1183	the Pearson correlation	1161:1183	the Pearson correlation	1161:1183	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	0	47	theme	Polysaccharides	77:91	arg1	Characterization					13:28	Characterization	13:28	Characterization	13:28	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	0	47	theme	Polysaccharides	77:91	arg1	Activity					53:60	Anti-Complementary Activity	34:60	Anti-Complementary Activity	34:60	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	0	47	theme	Polysaccharides	77:91	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	0	48	from	militaris	108:116	arg1	Characterization					13:28	Characterization	13:28	Characterization	13:28	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	0	48	from	militaris	108:116	arg1	Activity					53:60	Anti-Complementary Activity	34:60	Anti-Complementary Activity	34:60	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	0	48	from	militaris	108:116	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	4	49	theme	complement	884:893	arg1	activation					895:904	complement activation	884:904	complement activation	884:904	The evaluation of anti-complementary activity demonstrated that the three polysaccharides significantly inhibited complement activation through the classical pathway and alternative pathway.
36365633	1	50	theme	novel	159:163	arg1	polysaccharides					165:179	the three novel polysaccharides	149:179	the three novel polysaccharides from Cordyceps militaris	149:204	This investigation focuses on the three novel polysaccharides from Cordyceps militaris and then discusses their characterization and anti-complementary activity.
36365633	7	51	theme	potential	1423:1431	arg1	polysaccharides					1403:1417	the three novel polysaccharides	1387:1417	the three novel polysaccharides	1387:1417	These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.
36365633	7	51	theme	potential	1423:1431	arg1	candidates					1433:1442	potential candidates	1423:1442	potential candidates for anti-complementary drugs	1423:1471	These results suggested that the three novel polysaccharides are potential candidates for anti-complementary drugs.
36365633	0	52	theme	Novel	71:75	arg1	Polysaccharides					77:91	Three Novel Polysaccharides	65:91	Three Novel Polysaccharides	65:91	Preparation, Characterization and Anti-Complementary Activity of Three Novel Polysaccharides from Cordyceps militaris.
36365633	6	53	theme	network	1189:1195	arg1	analysis					1149:1156	The analysis	1145:1156	The analysis of the Pearson correlation and network	1145:1195	The analysis of the Pearson correlation and network confirmed that the ribose, glucuronic acid and galacturonic acid composition were negatively correlated with the anti-complementary activity of polysaccharides.
36365633	5	54	theme	B	1069:1069	arg1	components					1078:1087	C2, C5, C9, factor B, factor B, and P components	1040:1087	C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system	1040:1142	Preliminary mechanism studies indicated that CMP-1, CMP-2 and CMP-3 acted with C2, C5, C9, factor B, factor B, and P components in the overactivation cascade of the complement system.
36365633	1	55	from	militaris	196:204	arg1	polysaccharides					165:179	the three novel polysaccharides	149:179	the three novel polysaccharides from Cordyceps militaris	149:204	This investigation focuses on the three novel polysaccharides from Cordyceps militaris and then discusses their characterization and anti-complementary activity.
35387090	7	0	theme	glycan	1696:1701	arg1	profile					1685:1691	similar metabolic profile	1667:1691	similar metabolic profile of glycan	1667:1701	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	5	1	from	strains	1085:1091	arg1	STs					1070:1072	7 STs	1068:1072	7 STs from 31 Li strains, and no homologous recombination event of genes	1068:1139	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	1	from	strains	1085:1091	arg1	STs					1029:1031	20 STs	1026:1031	20 STs derived from 33 Uighur strains	1026:1062	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	3	2	theme	present	511:517	arg1	study					519:523	the present study	507:523	the present study	507:523	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	5	3	theme	genes	1135:1139	arg1	strains					1085:1091	31 Li strains	1079:1091	31 Li strains	1079:1091	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	3	theme	genes	1135:1139	arg1	event					1126:1130	no homologous recombination event	1098:1130	no homologous recombination event of genes	1098:1139	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	7	4	theme	similar	1667:1673	arg1	profile					1685:1691	similar metabolic profile	1667:1691	similar metabolic profile of glycan	1667:1701	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	3	5	theme	groups	677:682	arg1	pairs					657:661	31 healthy mother-infant pairs	632:661	31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively	632:727	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	5	6	theme	recombination	1112:1124	arg1	event					1126:1130	no homologous recombination event	1098:1130	no homologous recombination event of genes	1098:1139	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	0	7	theme	Ethnic	0:5	arg1	Specificity					7:17	Ethnic Specificity	0:17	Ethnic Specificity of Species	0:28	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	4	8	dep	composition	757:767	arg1	the					753:755	the	753:755	the	753:755	The result showed that the composition and number of Lactobacillus species between the two ethnic groups varied.
35387090	4	9	theme	ethnic	821:826	arg1	groups					828:833	the two ethnic groups	813:833	the two ethnic groups	813:833	The result showed that the composition and number of Lactobacillus species between the two ethnic groups varied.
35387090	6	10	theme	vertical	1423:1430	arg1	transfer					1432:1439	vertical transfer	1423:1439	vertical transfer of Lactobacillus	1423:1456	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	6	11	theme	go-EBURST	1200:1208	arg1	analysis					1210:1217	A go-EBURST analysis	1198:1217	A go-EBURST analysis	1198:1217	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	6	12	theme	mother-infant	1250:1262	arg1	pairs					1264:1268	a few mother-infant pairs	1244:1268	a few mother-infant pairs in which more than one STs were detected	1244:1309	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	3	13	theme	Lactobacillus	547:559	arg1	species					561:567	16 former Lactobacillus species	537:567	16 former Lactobacillus species (365 strains)	537:581	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	3	13	theme	Lactobacillus	547:559	arg1	strains					574:580	365 strains	570:580	365 strains	570:580	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	7	14	theme	ethnic	1529:1534	arg1	group					1536:1540	the same ethnic group	1520:1540	the same ethnic group	1520:1540	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	5	15	from	event	1126:1130	arg1	STs					1070:1072	7 STs	1068:1072	7 STs from 31 Li strains, and no homologous recombination event of genes	1068:1139	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	15	from	event	1126:1130	arg1	STs					1029:1031	20 STs	1026:1031	20 STs derived from 33 Uighur strains	1026:1062	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	1	16	theme	potential	207:215	arg1	bacteria					227:234	potential probiotic bacteria	207:234	potential probiotic bacteria	207:234	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	5	17	dep	types	959:963	arg1	ST					966:967	ST	966:967	ST	966:967	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	18	theme	representative	903:916	arg1	strains					918:924	64 representative strains	900:924	64 representative strains	900:924	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	7	19	theme	phylogenetic	1620:1631	arg1	clade					1633:1637	a specific phylogenetic clade	1609:1637	a specific phylogenetic clade	1609:1637	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	7	20	theme	L.	1493:1494	arg1	isolates					1506:1513	L. paracasei isolates	1493:1513	L. paracasei isolates from the same ethnic group	1493:1540	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	0	21	from	Pairs	101:105	arg1	Composition					41:51	Strain Composition	34:51	Strain Composition	34:51	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	0	21	from	Pairs	101:105	arg1	Specificity					7:17	Ethnic Specificity	0:17	Ethnic Specificity of Species	0:28	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	0	21	from	Pairs	101:105	arg1	Populations					70:80	Lactobacillus Populations	56:80	Lactobacillus Populations From Mother-Infant Pairs	56:105	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	0	22	theme	Lactobacillus	56:68	arg1	Populations					70:80	Lactobacillus Populations	56:80	Lactobacillus Populations From Mother-Infant Pairs	56:105	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	3	23	theme	species	590:596	arg1	total					528:532	a total	526:532	a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains)	526:610	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	6	24	theme	strain	1465:1470	arg1	level					1472:1476	the strain level	1461:1476	the strain level	1461:1476	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	5	25	theme	Lacticaseibacillus	853:870	arg1	strains					882:888	106 Lacticaseibacillus paracasei strains	849:888	106 Lacticaseibacillus paracasei strains isolated	849:897	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	26	theme	different	1173:1181	arg1	groups					1190:1195	different ethnic groups	1173:1195	different ethnic groups	1173:1195	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	8	27	from	perspective	1904:1914	arg1	populations					1883:1893	very homogenous localized populations	1857:1893	very homogenous localized populations from the perspective of maternal and child health	1857:1943	Our study provides evidence for the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health.
35387090	5	28	theme	groups	1190:1195	arg1	strains					1162:1168	strains	1162:1168	strains of different ethnic groups	1162:1195	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	8	29	theme	localized	1873:1881	arg1	populations					1883:1893	very homogenous localized populations	1857:1893	very homogenous localized populations from the perspective of maternal and child health	1857:1943	Our study provides evidence for the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health.
35387090	5	30	theme	sequence	950:957	arg1	types					959:963	27 sequence types	947:963	27 sequence types (ST)	947:968	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	0	31	from	Composition	41:51	arg1	Pairs					101:105	Mother-Infant Pairs	87:105	Mother-Infant Pairs	87:105	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	8	32	theme	health	1938:1943	arg1	perspective					1904:1914	the perspective	1900:1914	the perspective of maternal and child health	1900:1943	Our study provides evidence for the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health.
35387090	2	33	theme	species	447:453	arg1	terms					438:442	terms	438:442	terms of species and strain composition	438:476	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	6	34	theme	same	1343:1346	arg1	pair					1362:1365	the same mother-infant pair	1339:1365	the same mother-infant pair	1339:1365	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	1	35	theme	lactation	265:273	arg1	stage					275:279	the lactation stage	261:279	the lactation stage	261:279	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	6	36	from	cases	1405:1409	arg1	monophyletic					1384:1395	monophyletic	1384:1395	monophyletic	1384:1395	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	8	37	theme	maternal	1919:1926	arg1	health					1938:1943	maternal and child health	1919:1943	health	1938:1943	Our study provides evidence for the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health.
35387090	6	38	theme	paracasei	1315:1323	arg1	isolates					1325:1332	L. paracasei isolates	1312:1332	L. paracasei isolates from the same mother-infant pair	1312:1365	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	8	39	theme	child	1932:1936	arg1	health					1938:1943	maternal and child health	1919:1943	health	1938:1943	Our study provides evidence for the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health.
35387090	5	40	theme	multilocus	982:991	arg1	MLST					1010:1013	MLST	1010:1013	MLST	1010:1013	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	40	theme	multilocus	982:991	arg1	typing					1002:1007	multilocus sequence typing	982:1007	multilocus sequence typing (MLST)	982:1014	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	2	41	theme	strain	459:464	arg1	composition					466:476	strain composition	459:476	strain composition	459:476	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	4	42	theme	species	797:803	arg1	composition					757:767	composition	757:767	composition	757:767	The result showed that the composition and number of Lactobacillus species between the two ethnic groups varied.
35387090	4	42	theme	species	797:803	arg1	number					773:778	number	773:778	number	773:778	The result showed that the composition and number of Lactobacillus species between the two ethnic groups varied.
35387090	5	43	theme	Li	1082:1083	arg1	strains					1085:1091	31 Li strains	1079:1091	31 Li strains	1079:1091	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	7	44	theme	large	1757:1761	arg1	degree					1763:1768	a large degree	1755:1768	a large degree	1755:1768	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	3	45	theme	mother-infant	643:655	arg1	pairs					657:661	31 healthy mother-infant pairs	632:661	31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively	632:727	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	2	46	theme	symbiont	318:325	arg1	lactobacilli					327:338	facultative symbiont lactobacilli	306:338	facultative symbiont lactobacilli	306:338	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	1	47	theme	probiotic	217:225	arg1	bacteria					227:234	potential probiotic bacteria	207:234	potential probiotic bacteria	207:234	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	6	48	from	pair	1362:1365	arg1	isolates					1325:1332	L. paracasei isolates	1312:1332	L. paracasei isolates from the same mother-infant pair	1312:1365	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	1	49	theme	maternal	153:160	arg1	source					197:202	the principal source	183:202	the principal source of potential probiotic bacteria in the infant gut	183:252	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	1	49	theme	maternal	153:160	arg1	gut					162:164	The maternal gut	149:164	The maternal gut	149:164	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	2	50	from	specificity	423:433	arg1	terms					438:442	terms	438:442	terms of species and strain composition	438:476	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	2	50	from	specificity	423:433	arg1	cohorts					495:501	mother-infant cohorts	481:501	mother-infant cohorts	481:501	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	5	51	attach	derived	1033:1039	arg2	STs					1029:1031	20 STs	1026:1031	20 STs derived from 33 Uighur strains	1026:1062	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	51	attach	derived	1033:1039	arg1	strains					1056:1062	33 Uighur strains	1046:1062	33 Uighur strains	1046:1062	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	3	52	theme	ethnic	670:675	arg1	groups					677:682	two ethnic groups	666:682	two ethnic groups	666:682	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	2	53	theme	vertical	356:363	arg1	transmission					365:376	vertical transmission	356:376	vertical transmission from mother to infant	356:398	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	1	54	theme	infant	243:248	arg1	gut					250:252	the infant gut	239:252	the infant gut	239:252	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	7	55	theme	metabolic	1675:1683	arg1	profile					1685:1691	similar metabolic profile	1667:1691	similar metabolic profile of glycan	1667:1701	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	2	56	from	mother	383:388	arg1	transmission					365:376	vertical transmission	356:376	vertical transmission from mother to infant	356:398	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	6	57	theme	Lactobacillus	1444:1456	arg1	transfer					1432:1439	vertical transfer	1423:1439	vertical transfer of Lactobacillus	1423:1456	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	3	58	theme	species	561:567	arg1	total					528:532	a total	526:532	a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains)	526:610	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	0	59	theme	Sequence	132:139	arg1	Typing					141:146	Multilocus Sequence Typing	121:146	Multilocus Sequence Typing	121:146	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	3	60	theme	former	540:545	arg1	species					561:567	16 former Lactobacillus species	537:567	16 former Lactobacillus species (365 strains)	537:581	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	3	60	theme	former	540:545	arg1	strains					574:580	365 strains	570:580	365 strains	570:580	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	1	61	theme	principal	187:195	arg1	source					197:202	the principal source	183:202	the principal source of potential probiotic bacteria in the infant gut	183:252	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	1	61	theme	principal	187:195	arg1	gut					162:164	The maternal gut	149:164	The maternal gut	149:164	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	7	62	theme	same	1524:1527	arg1	group					1536:1540	the same ethnic group	1520:1540	the same ethnic group	1520:1540	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	6	63	theme	few	1246:1248	arg1	pairs					1264:1268	a few mother-infant pairs	1244:1268	a few mother-infant pairs in which more than one STs were detected	1244:1309	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	7	64	theme	paracasei	1496:1504	arg1	isolates					1506:1513	L. paracasei isolates	1493:1513	L. paracasei isolates from the same ethnic group	1493:1540	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	8	65	theme	probiotic	1835:1843	arg1	development					1807:1817	the development	1803:1817	the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health	1803:1943	Our study provides evidence for the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health.
35387090	1	66	from	source	197:202	arg1	gut					250:252	the infant gut	239:252	the infant gut	239:252	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	7	67	theme	clonal	1642:1647	arg1	CC					1658:1659	CC	1658:1659	CC	1658:1659	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	7	67	theme	clonal	1642:1647	arg1	complex					1649:1655	clonal complex	1642:1655	clonal complex (CC) with similar metabolic profile of glycan	1642:1701	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	7	68	from	another	1577:1583	arg1	strains					1564:1570	strains	1564:1570	strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan	1564:1701	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	0	69	theme	Populations	70:80	arg1	Composition					41:51	Strain Composition	34:51	Strain Composition	34:51	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	0	69	theme	Populations	70:80	arg1	Specificity					7:17	Ethnic Specificity	0:17	Ethnic Specificity of Species	0:28	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	0	70	theme	Mother-Infant	87:99	arg1	Pairs					101:105	Mother-Infant Pairs	87:105	Mother-Infant Pairs	87:105	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	5	71	theme	paracasei	872:880	arg1	strains					882:888	106 Lacticaseibacillus paracasei strains	849:888	106 Lacticaseibacillus paracasei strains isolated	849:897	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	7	72	theme	specific	1611:1618	arg1	clade					1633:1637	a specific phylogenetic clade	1609:1637	a specific phylogenetic clade	1609:1637	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	2	73	theme	mother-infant	481:493	arg1	cohorts					495:501	mother-infant cohorts	481:501	mother-infant cohorts	481:501	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	0	74	from	Specificity	7:17	arg1	Pairs					101:105	Mother-Infant Pairs	87:105	Mother-Infant Pairs	87:105	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	5	75	theme	ethnic	1183:1188	arg1	groups					1190:1195	different ethnic groups	1173:1195	different ethnic groups	1173:1195	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	6	76	theme	mother-infant	1348:1360	arg1	pair					1362:1365	the same mother-infant pair	1339:1365	the same mother-infant pair	1339:1365	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	5	77	theme	Uighur	1049:1054	arg1	strains					1056:1062	33 Uighur strains	1046:1062	33 Uighur strains	1046:1062	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	7	78	from	group	1536:1540	arg1	isolates					1506:1513	L. paracasei isolates	1493:1513	L. paracasei isolates from the same ethnic group	1493:1540	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	7	79	with	clade	1633:1637	arg1	profile					1685:1691	similar metabolic profile	1667:1691	similar metabolic profile of glycan	1667:1701	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	7	80	with	complex	1649:1655	arg1	profile					1685:1691	similar metabolic profile	1667:1691	similar metabolic profile of glycan	1667:1701	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	0	81	theme	Species	22:28	arg1	Composition					41:51	Strain Composition	34:51	Strain Composition	34:51	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	0	81	theme	Species	22:28	arg1	Specificity					7:17	Ethnic Specificity	0:17	Ethnic Specificity of Species	0:28	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	2	82	theme	ethnic	416:421	arg1	specificity					423:433	the ethnic specificity	412:433	the ethnic specificity in terms of species and strain composition in mother-infant cohorts	412:501	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	0	83	theme	Strain	34:39	arg1	Composition					41:51	Strain Composition	34:51	Strain Composition	34:51	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35387090	5	84	theme	homologous	1101:1110	arg1	event					1126:1130	no homologous recombination event	1098:1130	no homologous recombination event of genes	1098:1139	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	6	85	from	monophyletic	1384:1395	arg1	cases					1405:1409	most cases	1400:1409	most cases	1400:1409	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	2	86	theme	composition	466:476	arg1	terms					438:442	terms	438:442	terms of species and strain composition	438:476	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	8	87	theme	homogenous	1862:1871	arg1	populations					1883:1893	very homogenous localized populations	1857:1893	very homogenous localized populations from the perspective of maternal and child health	1857:1943	Our study provides evidence for the development of personalized probiotic tailored to very homogenous localized populations from the perspective of maternal and child health.
35387090	6	88	theme	L.	1312:1313	arg1	isolates					1325:1332	L. paracasei isolates	1312:1332	L. paracasei isolates from the same mother-infant pair	1312:1365	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	5	89	theme	sequence	993:1000	arg1	MLST					1010:1013	MLST	1010:1013	MLST	1010:1013	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	5	89	theme	sequence	993:1000	arg1	typing					1002:1007	multilocus sequence typing	982:1007	multilocus sequence typing (MLST)	982:1014	Among 106 Lacticaseibacillus paracasei strains isolated, 64 representative strains were classified into 27 sequence types (ST) by means of multilocus sequence typing (MLST), of which 20 STs derived from 33 Uighur strains and 7 STs from 31 Li strains, and no homologous recombination event of genes was detected between strains of different ethnic groups.
35387090	7	90	theme	ethnic	1733:1738	arg1	specificity					1740:1750	ethnic specificity	1733:1750	ethnic specificity	1733:1750	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	3	91	theme	healthy	635:641	arg1	pairs					657:661	31 healthy mother-infant pairs	632:661	31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively	632:727	In the present study, a total of 16 former Lactobacillus species (365 strains) and 11 species (280 strains) were retrieved from 31 healthy mother-infant pairs of two ethnic groups, which have never intermarried, respectively.
35387090	4	92	theme	Lactobacillus	783:795	arg1	species					797:803	Lactobacillus species	783:803	Lactobacillus species	783:803	The result showed that the composition and number of Lactobacillus species between the two ethnic groups varied.
35387090	6	93	theme	most	1400:1403	arg1	cases					1405:1409	most cases	1400:1409	most cases	1400:1409	A go-EBURST analysis revealed that except for a few mother-infant pairs in which more than one STs were detected, L. paracasei isolates from the same mother-infant pair were found to be monophyletic in most cases, confirming vertical transfer of Lactobacillus at the strain level.
35387090	7	94	theme	specificity	1740:1750	arg1	hypothesis					1719:1728	the hypothesis	1715:1728	the hypothesis of ethnic specificity to a large degree	1715:1768	More notably, L. paracasei isolates from the same ethnic group were more likely than strains from another to be incorporated into a specific phylogenetic clade or clonal complex (CC) with similar metabolic profile of glycan, supporting the hypothesis of ethnic specificity to a large degree.
35387090	2	95	theme	facultative	306:316	arg1	lactobacilli					327:338	facultative symbiont lactobacilli	306:338	facultative symbiont lactobacilli	306:338	It is not clear whether facultative symbiont lactobacilli strictly follow vertical transmission from mother to infant and display the ethnic specificity in terms of species and strain composition in mother-infant cohorts.
35387090	1	96	theme	bacteria	227:234	arg1	source					197:202	the principal source	183:202	the principal source of potential probiotic bacteria in the infant gut	183:252	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	1	96	theme	bacteria	227:234	arg1	gut					162:164	The maternal gut	149:164	The maternal gut	149:164	The maternal gut is thought to be the principal source of potential probiotic bacteria in the infant gut during the lactation stage.
35387090	0	97	theme	Multilocus	121:130	arg1	Typing					141:146	Multilocus Sequence Typing	121:146	Multilocus Sequence Typing	121:146	Ethnic Specificity of Species and Strain Composition of Lactobacillus Populations From Mother-Infant Pairs, Uncovered by Multilocus Sequence Typing.
35163046	6	0	theme	activity	883:890	arg1	post-surgery					892:903	brain neuronal activity post-surgery	868:903	brain neuronal activity post-surgery	868:903	Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery.
35163046	6	1	theme	brain	868:872	arg1	post-surgery					892:903	brain neuronal activity post-surgery	868:903	brain neuronal activity post-surgery	868:903	Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery.
35163046	10	2	theme	action	1537:1542	arg1	mode					1529:1532	the surgery mode	1517:1532	the surgery mode of action	1517:1542	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	7	3	theme	gut	1085:1087	arg1	signaling					1089:1097	gut signaling	1085:1097	gut signaling	1085:1097	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	6	4	theme	inflammatory	794:805	arg1	markers					807:813	inflammatory markers	794:813	inflammatory markers	794:813	Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery.
35163046	8	5	theme	serine	1136:1141	arg1	profiles					1182:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles	1100:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats	1100:1202	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	8	6	from	profiles	1182:1189	arg1	rats					1199:1202	RYGB rats	1194:1202	RYGB rats	1194:1202	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	8	7	theme	glycine	1144:1150	arg1	profiles					1182:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles	1100:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats	1100:1202	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	7	8	from	Bacteroidota	965:976	arg1	animals					1014:1020	RYGB animals	1009:1020	RYGB animals	1009:1020	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	5	9	theme	RYGB	635:638	arg1	groups					680:685	RYGB, sham pair-fed, and sham ad libitum-fed groups	635:685	RYGB, sham pair-fed, and sham ad libitum-fed groups	635:685	RYGB, sham pair-fed, and sham ad libitum-fed groups further continued on the diet after recovery.
35163046	10	10	theme	multi-modal	1362:1372	arg1	approach					1374:1381	Our multi-modal approach	1358:1381	Our multi-modal approach	1358:1381	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	8	11	theme	reverted	1223:1230	arg1	risk					1240:1243	reverted obesity risk	1223:1243	reverted obesity risk	1223:1243	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	7	12	theme	nervous	1060:1066	arg1	ENS					1076:1078	ENS	1076:1078	ENS	1076:1078	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	7	12	theme	nervous	1060:1066	arg1	system					1068:1073	the altered enteric nervous system	1040:1073	the altered enteric nervous system (ENS)	1040:1079	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	8	13	theme	plasma	1175:1180	arg1	profiles					1182:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles	1100:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats	1100:1202	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	8	14	theme	risk	1240:1243	arg1	suggestive					1209:1218	suggestive	1209:1218	suggestive	1209:1218	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	7	15	theme	altered	1044:1050	arg1	ENS					1076:1078	ENS	1076:1078	ENS	1076:1078	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	7	15	theme	altered	1044:1050	arg1	system					1068:1073	the altered enteric nervous system	1040:1073	the altered enteric nervous system (ENS)	1040:1079	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	1	16	theme	weight	198:203	arg1	loss					205:208	weight loss	198:208	weight loss	198:208	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	7	17	theme	Enterococcus	982:993	arg1	abundances					995:1004	Enterococcus abundances	982:1004	Enterococcus abundances	982:1004	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	5	18	theme	libitum-fed	668:678	arg1	groups					680:685	RYGB, sham pair-fed, and sham ad libitum-fed groups	635:685	RYGB, sham pair-fed, and sham ad libitum-fed groups	635:685	RYGB, sham pair-fed, and sham ad libitum-fed groups further continued on the diet after recovery.
35163046	1	19	theme	Roux-en-Y	128:136	arg1	surgery					160:166	Roux-en-Y gastric bypass (RYGB) surgery	128:166	Roux-en-Y gastric bypass (RYGB) surgery	128:166	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	0	20	theme	High	47:50	arg1	Diet					67:70	High Liquid Sucrose Diet	47:70	High Liquid Sucrose Diet	47:70	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	1	21	theme	bypass	146:151	arg1	surgery					160:166	Roux-en-Y gastric bypass (RYGB) surgery	128:166	Roux-en-Y gastric bypass (RYGB) surgery	128:166	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	2	22	from	malabsorption	299:311	arg1	patients					347:354	patients	347:354	patients	347:354	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	2	22	from	malabsorption	299:311	arg1	%					342:342	up to 50%	334:342	up to 50% of patients	334:354	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	0	23	theme	Sucrose	59:65	arg1	Diet					67:70	High Liquid Sucrose Diet	47:70	High Liquid Sucrose Diet	47:70	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	9	24	from	changes	1336:1342	arg1	liver					1351:1355	the liver	1347:1355	the liver	1347:1355	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	10	25	theme	systemic	1399:1406	arg1	changes					1408:1414	complex systemic changes	1391:1414	complex systemic changes after RYGB surgery	1391:1433	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	9	26	theme	liquid	1260:1265	arg1	diet					1275:1278	liquid sucrose diet	1260:1278	liquid sucrose diet	1260:1278	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	2	27	theme	additional	379:388	arg1	approaches					402:411	additional therapeutic approaches	379:411	additional therapeutic approaches	379:411	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	0	28	theme	Gut	81:83	arg1	Alterations					115:125	Gut Microbiota-Related Systematic Alterations	81:125	Gut Microbiota-Related Systematic Alterations	81:125	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	9	29	theme	diet	1275:1278	arg1	impact					1250:1255	The impact	1246:1255	The impact of liquid sucrose diet and caloric restriction	1246:1302	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	7	30	theme	fecal	913:917	arg1	GABA					936:939	GABA	936:939	GABA	936:939	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	7	30	theme	fecal	913:917	arg1	4-aminobutyrate					919:933	Higher fecal 4-aminobutyrate	906:933	Higher fecal 4-aminobutyrate (GABA)	906:940	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	0	31	theme	Roux-En-Y	0:8	arg1	Surgery					32:38	Roux-En-Y Gastric Bypass (RYGB) Surgery	0:38	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet	0:70	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	9	32	theme	caloric	1284:1290	arg1	restriction					1292:1302	caloric restriction	1284:1302	caloric restriction	1284:1302	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	4	33	theme	high	553:556	arg1	sucrose					565:571	high liquid sucrose	553:571	high liquid sucrose	553:571	After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery.
35163046	2	34	dep	50	340:341	arg1	to					337:338	to	337:338	to	337:338	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	0	35	theme	Bypass	18:23	arg1	Surgery					32:38	Roux-En-Y Gastric Bypass (RYGB) Surgery	0:38	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet	0:70	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	10	36	theme	potential	1454:1462	arg1	targets					1476:1482	potential therapeutic targets	1454:1482	potential therapeutic targets in the gut-brain system to mimic the surgery mode of action	1454:1542	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	6	37	theme	plasma	834:839	arg1	metabolites					841:851	plasma metabolites	834:851	plasma metabolites	834:851	Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery.
35163046	3	38	theme	rat	493:495	arg1	model					505:509	a liquid sucrose diet-induced rat obesity model	463:509	a liquid sucrose diet-induced rat obesity model	463:509	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	9	39	theme	acid	1331:1334	arg1	changes					1336:1342	fatty acid changes	1325:1342	fatty acid changes in the liver	1325:1355	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	3	40	theme	sucrose	472:478	arg1	model					505:509	a liquid sucrose diet-induced rat obesity model	463:509	a liquid sucrose diet-induced rat obesity model	463:509	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	0	41	dep	Bypass	18:23	arg1	RYGB					26:29	RYGB	26:29	RYGB	26:29	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	10	42	theme	surgery	1521:1527	arg1	mode					1529:1532	the surgery mode	1517:1532	the surgery mode of action	1517:1542	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	8	43	theme	Favorable	1100:1108	arg1	protein					1121:1127	Favorable C-reactive protein	1100:1127	Favorable C-reactive protein (CRP)	1100:1133	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	8	43	theme	Favorable	1100:1108	arg1	CRP					1130:1132	CRP	1130:1132	CRP	1130:1132	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	1	44	theme	co-morbidities	229:242	arg1	improvement					214:224	improvement	214:224	improvement	214:224	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	1	44	theme	co-morbidities	229:242	arg1	loss					205:208	weight loss	198:208	weight loss	198:208	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	8	45	theme	protein	1121:1127	arg1	profiles					1182:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles	1100:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats	1100:1202	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	6	46	theme	neuronal	874:881	arg1	post-surgery					892:903	brain neuronal activity post-surgery	868:903	brain neuronal activity post-surgery	868:903	Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery.
35163046	5	47	theme	pair-fed	646:653	arg1	RYGB					635:638	RYGB	635:638	RYGB	635:638	RYGB, sham pair-fed, and sham ad libitum-fed groups further continued on the diet after recovery.
35163046	2	48	theme	Chronic	269:275	arg1	complications					277:289	Chronic complications	269:289	Chronic complications such as malabsorption of micronutrients in up to 50% of patients	269:354	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	2	48	theme	Chronic	269:275	arg1	malabsorption					299:311	malabsorption	299:311	malabsorption of micronutrients in up to 50% of patients	299:354	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	8	49	theme	RYGB	1194:1197	arg1	rats					1199:1202	RYGB rats	1194:1202	RYGB rats	1194:1202	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	6	50	theme	microbiota	770:779	arg1	composition					781:791	microbiota composition	770:791	microbiota composition	770:791	Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery.
35163046	2	51	theme	micronutrients	316:329	arg1	malabsorption					299:311	malabsorption	299:311	malabsorption of micronutrients in up to 50% of patients	299:354	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	7	52	theme	enteric	1052:1058	arg1	ENS					1076:1078	ENS	1076:1078	ENS	1076:1078	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	7	52	theme	enteric	1052:1058	arg1	system					1068:1073	the altered enteric nervous system	1040:1073	the altered enteric nervous system (ENS)	1040:1079	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	4	53	theme	sham	621:624	arg1	surgery					626:632	RYGB or control sham surgery	605:632	surgery	626:632	After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery.
35163046	8	54	theme	3-hydroxybutyrate	1157:1173	arg1	profiles					1182:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles	1100:1189	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats	1100:1202	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	8	55	theme	obesity	1232:1238	arg1	risk					1240:1243	reverted obesity risk	1223:1243	reverted obesity risk	1223:1243	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	1	56	from	improvement	214:224	arg1	successful					184:193	successful	184:193	successful	184:193	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	7	57	theme	RYGB	1009:1012	arg1	animals					1014:1020	RYGB animals	1009:1020	RYGB animals	1009:1020	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	1	58	from	loss	205:208	arg1	successful					184:193	successful	184:193	successful	184:193	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	1	59	from	successful	184:193	arg1	improvement					214:224	improvement	214:224	improvement	214:224	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	1	59	from	successful	184:193	arg1	loss					205:208	weight loss	198:208	weight loss	198:208	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	0	60	theme	Liquid	52:57	arg1	Diet					67:70	High Liquid Sucrose Diet	47:70	High Liquid Sucrose Diet	47:70	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	1	61	theme	gastric	138:144	arg1	surgery					160:166	Roux-en-Y gastric bypass (RYGB) surgery	128:166	Roux-en-Y gastric bypass (RYGB) surgery	128:166	Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity.
35163046	10	62	from	targets	1476:1482	arg1	system					1501:1506	the gut-brain system to mimic the surgery mode of action	1487:1542	the gut-brain system to mimic the surgery mode of action	1487:1542	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	7	63	theme	higher	958:963	arg1	Bacteroidota					965:976	higher Bacteroidota	958:976	higher Bacteroidota	958:976	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	0	64	theme	Systematic	104:113	arg1	Alterations					115:125	Gut Microbiota-Related Systematic Alterations	81:125	Gut Microbiota-Related Systematic Alterations	81:125	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	10	65	dep	potential	1454:1462	arg1	therapeutic					1464:1474	therapeutic	1464:1474	therapeutic	1464:1474	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	10	66	theme	complex	1391:1397	arg1	changes					1408:1414	complex systemic changes	1391:1414	complex systemic changes after RYGB surgery	1391:1433	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	0	67	theme	Microbiota-Related	85:102	arg1	Alterations					115:125	Gut Microbiota-Related Systematic Alterations	81:125	Gut Microbiota-Related Systematic Alterations	81:125	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	9	68	theme	sucrose	1267:1273	arg1	diet					1275:1278	liquid sucrose diet	1260:1278	liquid sucrose diet	1260:1278	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	7	69	from	abundances	995:1004	arg1	animals					1014:1020	RYGB animals	1009:1020	RYGB animals	1009:1020	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	3	70	theme	surgery	444:450	arg1	effects					452:458	systemic RYGB surgery effects	430:458	systemic RYGB surgery effects	430:458	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	0	71	theme	Gastric	10:16	arg1	Surgery					32:38	Roux-En-Y Gastric Bypass (RYGB) Surgery	0:38	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet	0:70	Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations.
35163046	4	72	theme	liquid	558:563	arg1	sucrose					565:571	high liquid sucrose	553:571	high liquid sucrose	553:571	After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery.
35163046	10	73	theme	RYGB	1422:1425	arg1	surgery					1427:1433	RYGB surgery	1422:1433	RYGB surgery	1422:1433	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	3	74	theme	systemic	430:437	arg1	effects					452:458	systemic RYGB surgery effects	430:458	systemic RYGB surgery effects	430:458	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	9	75	theme	restriction	1292:1302	arg1	impact					1250:1255	The impact	1246:1255	The impact of liquid sucrose diet and caloric restriction	1246:1302	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	7	76	theme	Higher	906:911	arg1	GABA					936:939	GABA	936:939	GABA	936:939	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	7	76	theme	Higher	906:911	arg1	4-aminobutyrate					919:933	Higher fecal 4-aminobutyrate	906:933	Higher fecal 4-aminobutyrate (GABA)	906:940	Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and Enterococcus abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling.
35163046	3	77	theme	liquid	465:470	arg1	model					505:509	a liquid sucrose diet-induced rat obesity model	463:509	a liquid sucrose diet-induced rat obesity model	463:509	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	3	78	theme	RYGB	439:442	arg1	effects					452:458	systemic RYGB surgery effects	430:458	systemic RYGB surgery effects	430:458	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	2	79	theme	therapeutic	390:400	arg1	approaches					402:411	additional therapeutic approaches	379:411	additional therapeutic approaches	379:411	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	9	80	theme	fatty	1325:1329	arg1	changes					1336:1342	fatty acid changes	1325:1342	fatty acid changes in the liver	1325:1355	The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver.
35163046	10	81	theme	gut-brain	1491:1499	arg1	system					1501:1506	the gut-brain system to mimic the surgery mode of action	1487:1542	the gut-brain system to mimic the surgery mode of action	1487:1542	Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.
35163046	6	82	theme	Notable	733:739	arg1	alterations					741:751	Notable alterations	733:751	Notable alterations	733:751	Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery.
35163046	3	83	theme	obesity	497:503	arg1	model					505:509	a liquid sucrose diet-induced rat obesity model	463:509	a liquid sucrose diet-induced rat obesity model	463:509	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	4	84	theme	RYGB	605:608	arg1	surgery					626:632	RYGB or control sham surgery	605:632	surgery	626:632	After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery.
35163046	3	85	theme	diet-induced	480:491	arg1	model					505:509	a liquid sucrose diet-induced rat obesity model	463:509	a liquid sucrose diet-induced rat obesity model	463:509	We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model.
35163046	8	86	theme	C-reactive	1110:1119	arg1	protein					1121:1127	Favorable C-reactive protein	1100:1127	Favorable C-reactive protein (CRP)	1100:1133	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	8	86	theme	C-reactive	1110:1119	arg1	CRP					1130:1132	CRP	1130:1132	CRP	1130:1132	Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk.
35163046	4	87	theme	control	613:619	arg1	surgery					626:632	RYGB or control sham surgery	605:632	surgery	626:632	After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery.
35163046	2	88	theme	patients	347:354	arg1	patients					347:354	patients	347:354	patients	347:354	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35163046	2	88	theme	patients	347:354	arg1	%					342:342	up to 50%	334:342	up to 50% of patients	334:354	Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches.
35243299	5	0	dep	composition	697:707	arg1	e.g.					691:694	e.g.	691:694	e.g.	691:694	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	8	1	theme	ECM	1080:1082	arg1	research					1084:1091	ECM research	1080:1091	ECM research	1080:1091	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	10	2	theme	disease-specific	1348:1363	arg1	structures					1369:1378	disease-specific ECM structures	1348:1378	disease-specific ECM structures	1348:1378	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	1	3	theme	thousand	215:222	arg1	proteins					224:231	a thousand proteins	213:231	a thousand proteins	213:231	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	10	4	theme	ECM	1365:1367	arg1	structures					1369:1378	disease-specific ECM structures	1348:1378	disease-specific ECM structures	1348:1378	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	7	5	theme	proteins	953:960	arg1	arrangement					930:940	the spatiotemporal arrangement	911:940	the spatiotemporal arrangement of all ECM proteins	911:960	A detailed atlas deciphering the spatiotemporal arrangement of all ECM proteins is lacking.
35243299	7	6	theme	ECM	949:951	arg1	proteins					953:960	all ECM proteins	945:960	all ECM proteins	945:960	A detailed atlas deciphering the spatiotemporal arrangement of all ECM proteins is lacking.
35243299	3	7	theme	aberrant	521:528	arg1	behavior					535:542	aberrant cell behavior	521:542	aberrant cell behavior	521:542	The homeostatic balance of cell-ECM interactions can be lost, due to ageing, irritants or diseases, which results in aberrant cell behavior.
35243299	5	8	theme	topographical	746:758	arg1	cues					778:781	biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues	678:781	biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues	678:781	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	10	9	theme	disease-related	1432:1446	arg1	patterns					1452:1459	disease-related ECM patterns	1432:1459	disease-related ECM patterns reverting to a normal pattern	1432:1489	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	2	10	with	relationship	275:286	arg1	ECM					297:299	the ECM	293:299	the ECM	293:299	Cells engage in a reciprocal relationship with the ECM; they build, inhabit, maintain, and remodel the ECM, while, in turn, the ECM regulates their behavior.
35243299	10	11	theme	ECM	1448:1450	arg1	patterns					1452:1459	disease-related ECM patterns	1432:1459	disease-related ECM patterns reverting to a normal pattern	1432:1489	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	5	12	dep	biochemical	678:688	arg1	composition					697:707	composition	697:707	composition	697:707	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	5	13	theme	biophysical	711:721	arg1	cues					778:781	biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues	678:781	biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues	678:781	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	6	14	theme	ECM	866:868	arg1	topography					870:879	ECM topography	866:879	ECM topography	866:879	While advances have been made in many areas, we only have a very limited grasp of ECM topography.
35243299	8	15	theme	such	987:990	arg1	goal					1071:1074	a priority goal	1060:1074	a priority goal for ECM research	1060:1091	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	8	15	theme	such	987:990	arg1	atlas					1044:1048	such an extracellular matrix architecture (matritecture) atlas	987:1048	such an extracellular matrix architecture (matritecture) atlas	987:1048	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	9	16	theme	therapeutic	1225:1235	arg1	targets					1237:1243	therapeutic targets	1225:1243	therapeutic targets	1225:1243	In this commentary, we will discuss the need to resolve the spatiotemporal matritecture to identify potential disease triggers and therapeutic targets and present strategies to address this goal.
35243299	5	17	dep	structure	767:775	arg1	e.g.					761:764	e.g.	761:764	e.g.	761:764	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	10	18	dep	will	1325:1328	arg1	only					1334:1337	only	1334:1337	only	1334:1337	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	8	19	theme	matrix	1009:1014	arg1	architecture					1016:1027	an extracellular matrix architecture	992:1027	such an extracellular matrix architecture (matritecture) atlas	987:1048	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	8	19	theme	matrix	1009:1014	arg1	matritecture					1030:1041	matritecture	1030:1041	matritecture	1030:1041	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	10	20	theme	future	1399:1404	arg1	strategies					1406:1415	future strategies	1399:1415	future strategies	1399:1415	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	8	21	theme	extracellular	995:1007	arg1	architecture					1016:1027	an extracellular matrix architecture	992:1027	such an extracellular matrix architecture (matritecture) atlas	987:1048	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	8	21	theme	extracellular	995:1007	arg1	matritecture					1030:1041	matritecture	1030:1041	matritecture	1030:1041	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	6	22	theme	many	817:820	arg1	areas					822:826	many areas	817:826	many areas	817:826	While advances have been made in many areas, we only have a very limited grasp of ECM topography.
35243299	7	23	theme	spatiotemporal	915:928	arg1	arrangement					930:940	the spatiotemporal arrangement	911:940	the spatiotemporal arrangement of all ECM proteins	911:960	A detailed atlas deciphering the spatiotemporal arrangement of all ECM proteins is lacking.
35243299	8	24	theme	priority	1062:1069	arg1	goal					1071:1074	a priority goal	1060:1074	a priority goal for ECM research	1060:1091	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	8	24	theme	priority	1062:1069	arg1	atlas					1044:1048	such an extracellular matrix architecture (matritecture) atlas	987:1048	such an extracellular matrix architecture (matritecture) atlas	987:1048	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	7	25	theme	detailed	884:891	arg1	atlas					893:897	A detailed atlas	882:897	A detailed atlas deciphering the spatiotemporal arrangement of all ECM proteins	882:960	A detailed atlas deciphering the spatiotemporal arrangement of all ECM proteins is lacking.
35243299	5	26	dep	stiffness	730:738	arg1	e.g.					724:727	e.g.	724:727	e.g.	724:727	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	2	27	theme	reciprocal	264:273	arg1	relationship					275:286	a reciprocal relationship	262:286	a reciprocal relationship with the ECM	262:299	Cells engage in a reciprocal relationship with the ECM; they build, inhabit, maintain, and remodel the ECM, while, in turn, the ECM regulates their behavior.
35243299	1	28	theme	acellular	173:181	arg1	edifice					183:189	an acellular edifice	170:189	an acellular edifice built up by more than a thousand proteins and glycans	170:243	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	1	28	theme	acellular	173:181	arg1	matrix					156:161	the extracellular matrix	138:161	the extracellular matrix (ECM)	138:167	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	8	29	theme	architecture	1016:1027	arg1	goal					1071:1074	a priority goal	1060:1074	a priority goal for ECM research	1060:1091	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	8	29	theme	architecture	1016:1027	arg1	atlas					1044:1048	such an extracellular matrix architecture (matritecture) atlas	987:1048	such an extracellular matrix architecture (matritecture) atlas	987:1048	We feel that such an extracellular matrix architecture (matritecture) atlas should be a priority goal for ECM research.
35243299	1	30	theme	multicellular	100:112	arg1	organisms					114:122	multicellular organisms	100:122	multicellular organisms	100:122	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	3	31	theme	cell-ECM	431:438	arg1	interactions					440:451	cell-ECM interactions	431:451	cell-ECM interactions	431:451	The homeostatic balance of cell-ECM interactions can be lost, due to ageing, irritants or diseases, which results in aberrant cell behavior.
35243299	0	32	theme	matrix	40:45	arg1	architecture					47:58	the extracellular matrix architecture	22:58	the extracellular matrix architecture during health and disease	22:84	Matritecture: Mapping the extracellular matrix architecture during health and disease.
35243299	10	33	theme	matritecture	1306:1317	arg1	atlas					1319:1323	Such a detailed matritecture atlas	1290:1323	Such a detailed matritecture atlas	1290:1323	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	10	34	theme	normal	1476:1481	arg1	pattern					1483:1489	a normal pattern	1474:1489	a normal pattern	1474:1489	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	0	35	theme	extracellular	26:38	arg1	architecture					47:58	the extracellular matrix architecture	22:58	the extracellular matrix architecture during health and disease	22:84	Matritecture: Mapping the extracellular matrix architecture during health and disease.
35243299	9	36	theme	spatiotemporal	1154:1167	arg1	matritecture					1169:1180	the spatiotemporal matritecture	1150:1180	the spatiotemporal matritecture to identify potential disease triggers and therapeutic targets	1150:1243	In this commentary, we will discuss the need to resolve the spatiotemporal matritecture to identify potential disease triggers and therapeutic targets and present strategies to address this goal.
35243299	10	37	theme	detailed	1297:1304	arg1	atlas					1319:1323	Such a detailed matritecture atlas	1290:1323	Such a detailed matritecture atlas	1290:1323	Such a detailed matritecture atlas will not only identify disease-specific ECM structures but may also guide future strategies to restructure disease-related ECM patterns reverting to a normal pattern.
35243299	3	38	theme	cell	530:533	arg1	behavior					535:542	aberrant cell behavior	521:542	aberrant cell behavior	521:542	The homeostatic balance of cell-ECM interactions can be lost, due to ageing, irritants or diseases, which results in aberrant cell behavior.
35243299	5	39	theme	biochemical	678:688	arg1	cues					778:781	biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues	678:781	biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues	678:781	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	6	40	contain	have	837:840	arg1	we					829:830	we	829:830	we	829:830	While advances have been made in many areas, we only have a very limited grasp of ECM topography.
35243299	6	40	contain	have	837:840	arg2	grasp					857:861	a very limited grasp	842:861	a very limited grasp of ECM topography	842:879	While advances have been made in many areas, we only have a very limited grasp of ECM topography.
35243299	1	41	theme	extracellular	142:154	arg1	edifice					183:189	an acellular edifice	170:189	an acellular edifice built up by more than a thousand proteins and glycans	170:243	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	1	41	theme	extracellular	142:154	arg1	ECM					164:166	ECM	164:166	ECM	164:166	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	1	41	theme	extracellular	142:154	arg1	matrix					156:161	the extracellular matrix	138:161	the extracellular matrix (ECM)	138:167	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	3	42	theme	homeostatic	408:418	arg1	balance					420:426	The homeostatic balance	404:426	The homeostatic balance of cell-ECM interactions	404:451	The homeostatic balance of cell-ECM interactions can be lost, due to ageing, irritants or diseases, which results in aberrant cell behavior.
35243299	9	43	theme	potential	1194:1202	arg1	triggers					1212:1219	potential disease triggers	1194:1219	potential disease triggers	1194:1219	In this commentary, we will discuss the need to resolve the spatiotemporal matritecture to identify potential disease triggers and therapeutic targets and present strategies to address this goal.
35243299	6	44	theme	limited	849:855	arg1	grasp					857:861	a very limited grasp	842:861	a very limited grasp of ECM topography	842:879	While advances have been made in many areas, we only have a very limited grasp of ECM topography.
35243299	0	45	dep	Mapping	14:20	arg1	Matritecture					0:11	Matritecture	0:11	Matritecture	0:11	Matritecture: Mapping the extracellular matrix architecture during health and disease.
35243299	5	46	theme	cues	778:781	arg1	form					670:673	the form	666:673	the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues	666:781	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	9	47	theme	disease	1204:1210	arg1	triggers					1212:1219	potential disease triggers	1194:1219	potential disease triggers	1194:1219	In this commentary, we will discuss the need to resolve the spatiotemporal matritecture to identify potential disease triggers and therapeutic targets and present strategies to address this goal.
35243299	6	48	theme	topography	870:879	arg1	grasp					857:861	a very limited grasp	842:861	a very limited grasp of ECM topography	842:879	While advances have been made in many areas, we only have a very limited grasp of ECM topography.
35243299	4	49	theme	disease	577:583	arg1	progression					585:595	disease progression	577:595	disease progression	577:595	The ECM can suppress or promote disease progression, depending on the information relayed to cells.
35243299	1	50	from	cells	91:95	arg1	organisms					114:122	multicellular organisms	100:122	multicellular organisms	100:122	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
35243299	5	51	dep	biophysical	711:721	arg1	stiffness					730:738	stiffness	730:738	stiffness	730:738	Instructions come in the form of biochemical (e.g., composition), biophysical (e.g., stiffness), and topographical (e.g., structure) cues.
35243299	3	52	theme	interactions	440:451	arg1	balance					420:426	The homeostatic balance	404:426	The homeostatic balance of cell-ECM interactions	404:451	The homeostatic balance of cell-ECM interactions can be lost, due to ageing, irritants or diseases, which results in aberrant cell behavior.
35243299	1	53	theme	a	213:213	arg1	proteins					224:231	a thousand proteins	213:231	a thousand proteins	213:231	All cells in multicellular organisms are housed in the extracellular matrix (ECM), an acellular edifice built up by more than a thousand proteins and glycans.
36558996	0	0	from	Structure	12:20	arg1	continentalis					78:90	Aralia continentalis	71:90	Aralia continentalis	71:90	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	5	1	theme	analytical	765:774	arg1	results					776:782	The structural analytical results	750:782	The structural analytical results	750:782	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	1	2	theme	Kitigawa	240:247	arg1	stems					198:202	stems	198:202	stems	198:202	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	1	2	theme	Kitigawa	240:247	arg1	roots					191:195	roots	191:195	roots	191:195	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	2	3	dep	the	443:445	arg1	leaves					447:452	leaves	447:452	leaves	447:452	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	1	4	theme	acidic	159:164	arg1	polysaccharides					166:180	three neutral and three acidic polysaccharides	135:180	three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa	135:247	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	6	5	theme	antioxidants	1061:1072	arg1	screening					1019:1027	the screening	1015:1027	the screening of natural polysaccharide-based antioxidants	1015:1072	Our findings offer insights into the screening of natural polysaccharide-based antioxidants and provide a theoretical basis for the application of A. continentalis.
36558996	6	6	theme	continentalis	1132:1144	arg1	application					1114:1124	the application	1110:1124	the application of A. continentalis	1110:1144	Our findings offer insights into the screening of natural polysaccharide-based antioxidants and provide a theoretical basis for the application of A. continentalis.
36558996	3	7	theme	stronger	537:544	arg1	activity					558:565	stronger antioxidant activity	537:565	stronger antioxidant activity	537:565	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	3	8	theme	antioxidant	546:556	arg1	activity					558:565	stronger antioxidant activity	537:565	stronger antioxidant activity	537:565	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	2	9	dep	roots	352:356	arg1	the					348:350	the	348:350	the	348:350	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	6	10	theme	A.	1129:1130	arg1	continentalis					1132:1144	A. continentalis	1129:1144	A. continentalis	1129:1144	Our findings offer insights into the screening of natural polysaccharide-based antioxidants and provide a theoretical basis for the application of A. continentalis.
36558996	2	11	theme	analysis	269:276	arg1	results					254:260	The results	250:260	The results of the analysis of monosaccharide composition	250:306	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	2	12	from	the	443:445	arg1	polysaccharides					422:436	the polysaccharides	418:436	the polysaccharides from the leaves	418:452	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	2	12	from	the	443:445	arg1	they					405:408	they	405:408	they	405:408	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	5	13	theme	WACP	799:802	arg1	-A-c					806:809	WACP(R)-A-c	799:809	WACP(R)-A-c	799:809	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	0	14	from	Activity	38:45	arg1	continentalis					78:90	Aralia continentalis	71:90	Aralia continentalis	71:90	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	5	15	contain	contained	920:928	arg1	-A-d					915:918	WACP(L)-A-d	908:918	WACP(L)-A-d	908:918	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	15	contain	contained	920:928	arg2	RG-II					934:938	RG-II	934:938	RG-II	934:938	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	15	contain	contained	920:928	arg2	domains					973:979	rhamnogalacturonan I (RG-I) domains	945:979	rhamnogalacturonan I (RG-I) domains	945:979	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	15	contain	contained	920:928	arg2	HG					930:931	HG	930:931	HG	930:931	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	16	theme	rhamnogalacturonan	945:962	arg1	domains					973:979	rhamnogalacturonan I (RG-I) domains	945:979	rhamnogalacturonan I (RG-I) domains	945:979	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	2	17	from	roots	352:356	arg1	polysaccharides					327:341	the polysaccharides	323:341	the polysaccharides from the roots and stems	323:366	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	2	17	from	roots	352:356	arg1	similar					378:384	similar	378:384	similar	378:384	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	6	18	theme	theoretical	1088:1098	arg1	basis					1100:1104	a theoretical basis	1086:1104	a theoretical basis for the application of A. continentalis	1086:1144	Our findings offer insights into the screening of natural polysaccharide-based antioxidants and provide a theoretical basis for the application of A. continentalis.
36558996	5	19	contain	contained	856:864	arg2	rhamnogalacturonan					873:890	rhamnogalacturonan II	873:893	rhamnogalacturonan II (RG-II)	873:901	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	19	contain	contained	856:864	arg2	HG					866:867	HG	866:867	HG	866:867	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	19	contain	contained	856:864	arg1	-A-c					851:854	WACP(S)-A-c	844:854	WACP(S)-A-c	844:854	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	19	contain	contained	856:864	arg2	RG-II					896:900	RG-II	896:900	RG-II	896:900	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	20	theme	R	804:804	arg1	-A-c					806:809	WACP(R)-A-c	799:809	WACP(R)-A-c	799:809	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	3	21	theme	neutral	576:582	arg1	fractions					584:592	the neutral fractions	572:592	the neutral fractions	572:592	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	6	22	theme	polysaccharide-based	1040:1059	arg1	antioxidants					1061:1072	natural polysaccharide-based antioxidants	1032:1072	natural polysaccharide-based antioxidants	1032:1072	Our findings offer insights into the screening of natural polysaccharide-based antioxidants and provide a theoretical basis for the application of A. continentalis.
36558996	2	23	from	stems	362:366	arg1	polysaccharides					327:341	the polysaccharides	323:341	the polysaccharides from the roots and stems	323:366	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	2	23	from	stems	362:366	arg1	similar					378:384	similar	378:384	similar	378:384	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	2	24	theme	composition	296:306	arg1	analysis					269:276	the analysis	265:276	the analysis of monosaccharide composition	265:306	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	4	25	theme	acidic	643:648	arg1	fractions					665:673	the primary purified acidic polysaccharide fractions	622:673	the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d)	622:717	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	0	26	theme	Structure	12:20	arg1	Analysis					0:7	Analysis	0:7	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.	0:91	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	5	27	theme	S	849:849	arg1	-A-c					851:854	WACP(S)-A-c	844:854	WACP(S)-A-c	844:854	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	2	28	theme	monosaccharide	281:294	arg1	composition					296:306	monosaccharide composition	281:306	monosaccharide composition	281:306	The results of the analysis of monosaccharide composition indicated that the polysaccharides from the roots and stems were more similar to each other than they were to the polysaccharides from the leaves.
36558996	4	29	theme	purified	634:641	arg1	fractions					665:673	the primary purified acidic polysaccharide fractions	622:673	the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d)	622:717	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	0	30	theme	Activity	38:45	arg1	Analysis					0:7	Analysis	0:7	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.	0:91	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	0	31	from	continentalis	78:90	arg1	Structure					12:20	Structure	12:20	Structure	12:20	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	0	31	from	continentalis	78:90	arg1	Activity					38:45	Antioxidant Activity	26:45	Antioxidant Activity	26:45	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	0	31	from	continentalis	78:90	arg1	Polysaccharides					50:64	Polysaccharides	50:64	Polysaccharides from Aralia continentalis	50:90	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	4	32	dep	fractions	665:673	arg1	-A-d					713:716	-A-d	713:716	-A-d	713:716	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	4	32	dep	fractions	665:673	arg1	-A-c					683:686	-A-c	683:686	-A-c	683:686	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	4	32	dep	fractions	665:673	arg1	-A-c					696:699	-A-c	696:699	-A-c	696:699	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	0	33	theme	Antioxidant	26:36	arg1	Activity					38:45	Antioxidant Activity	26:45	Antioxidant Activity	26:45	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	3	34	contain	had	533:535	arg1	polysaccharides					517:531	the acidic polysaccharides	506:531	the acidic polysaccharides	506:531	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	3	34	contain	had	533:535	arg2	activity					558:565	stronger antioxidant activity	537:565	stronger antioxidant activity	537:565	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	1	35	dep	roots	191:195	arg1	the					187:189	the	187:189	the	187:189	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	4	36	theme	polysaccharide	650:663	arg1	fractions					665:673	the primary purified acidic polysaccharide fractions	622:673	the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d)	622:717	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	0	37	theme	Polysaccharides	50:64	arg1	Structure					12:20	Structure	12:20	Structure	12:20	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	0	37	theme	Polysaccharides	50:64	arg1	Activity					38:45	Antioxidant Activity	26:45	Antioxidant Activity	26:45	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	5	38	theme	WACP	908:911	arg1	-A-d					915:918	WACP(L)-A-d	908:918	WACP(L)-A-d	908:918	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	39	theme	I	964:964	arg1	domains					973:979	rhamnogalacturonan I (RG-I) domains	945:979	rhamnogalacturonan I (RG-I) domains	945:979	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	3	40	theme	antioxidant	468:478	arg1	results					480:486	The in vitro antioxidant results	455:486	The in vitro antioxidant results	455:486	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	5	41	theme	L	913:913	arg1	-A-d					915:918	WACP(L)-A-d	908:918	WACP(L)-A-d	908:918	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	6	42	theme	natural	1032:1038	arg1	antioxidants					1061:1072	natural polysaccharide-based antioxidants	1032:1072	natural polysaccharide-based antioxidants	1032:1072	Our findings offer insights into the screening of natural polysaccharide-based antioxidants and provide a theoretical basis for the application of A. continentalis.
36558996	1	43	from	leaves	209:214	arg1	polysaccharides					166:180	three neutral and three acidic polysaccharides	135:180	three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa	135:247	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	1	44	from	stems	198:202	arg1	polysaccharides					166:180	three neutral and three acidic polysaccharides	135:180	three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa	135:247	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	3	45	theme	in	459:460	arg1	results					480:486	The in vitro antioxidant results	455:486	The in vitro antioxidant results	455:486	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	3	46	dep	in	459:460	arg1	vitro					462:466	vitro	462:466	vitro	462:466	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
36558996	5	47	theme	WACP	844:847	arg1	-A-c					851:854	WACP(S)-A-c	844:854	WACP(S)-A-c	844:854	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	0	48	theme	Aralia	71:76	arg1	continentalis					78:90	Aralia continentalis	71:90	Aralia continentalis	71:90	Analysis of Structure and Antioxidant Activity of Polysaccharides from Aralia continentalis.
36558996	4	49	theme	enzymatic	730:738	arg1	analysis					740:747	enzymatic analysis	730:747	enzymatic analysis	730:747	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	1	50	from	roots	191:195	arg1	polysaccharides					166:180	three neutral and three acidic polysaccharides	135:180	three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa	135:247	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	1	51	theme	neutral	141:147	arg1	polysaccharides					166:180	three neutral and three acidic polysaccharides	135:180	three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa	135:247	We extracted, purified, and characterized three neutral and three acidic polysaccharides from the roots, stems, and leaves of Aralia continentalis Kitigawa.
36558996	4	52	theme	primary	626:632	arg1	fractions					665:673	the primary purified acidic polysaccharide fractions	622:673	the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d)	622:717	Therefore, we investigated the primary purified acidic polysaccharide fractions (WACP(R)-A-c, WACP(S)-A-c, and WACP(L)-A-d) by NMR and enzymatic analysis.
36558996	5	53	theme	RG-I	967:970	arg1	domains					973:979	rhamnogalacturonan I (RG-I) domains	945:979	rhamnogalacturonan I (RG-I) domains	945:979	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	54	theme	structural	754:763	arg1	results					776:782	The structural analytical results	750:782	The structural analytical results	750:782	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	55	contain	contained	811:819	arg1	-A-c					806:809	WACP(R)-A-c	799:809	WACP(R)-A-c	799:809	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	55	contain	contained	811:819	arg2	HG					839:840	HG	839:840	HG	839:840	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	5	55	contain	contained	811:819	arg2	homogalacturonan					821:836	homogalacturonan	821:836	homogalacturonan (HG)	821:841	The structural analytical results indicated that WACP(R)-A-c contained homogalacturonan (HG); WACP(S)-A-c contained HG and rhamnogalacturonan II (RG-II), and WACP(L)-A-d contained HG, RG-II, and rhamnogalacturonan I (RG-I) domains.
36558996	3	56	theme	acidic	510:515	arg1	polysaccharides					517:531	the acidic polysaccharides	506:531	the acidic polysaccharides	506:531	The in vitro antioxidant results demonstrated that the acidic polysaccharides had stronger antioxidant activity than the neutral fractions.
35505382	13	0	from	variation	2029:2037	arg1	composition					2052:2062	microbial composition	2042:2062	microbial composition	2042:2062	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	13	0	from	variation	2029:2037	arg1	ceca					2071:2074	the ceca	2067:2074	the ceca	2067:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	2	1	theme	broiler	428:434	arg1	growth					436:441	broiler growth	428:441	broiler growth	428:441	Recently, we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility, arabinoxylan fermentability and broiler growth.
35505382	13	2	from	ceca	2071:2074	arg1	%					2014:2014	47%	2012:2014	47% of the total variation in microbial composition in the ceca	2012:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	13	2	from	ceca	2071:2074	arg1	variation					2029:2037	the total variation	2019:2037	the total variation in microbial composition in the ceca	2019:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	5	3	from	enzymes	793:799	arg1	broilers					834:841	broilers	834:841	broilers	834:841	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	1	4	theme	enzyme	140:145	arg1	supplementation					147:161	Dietary fiber-degrading enzyme supplementation	116:161	Dietary fiber-degrading enzyme supplementation in broilers	116:173	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	2	5	theme	xylanase/glucanase	315:332	arg1	addition					334:341	xylanase/glucanase addition	315:341	xylanase/glucanase addition in wheat-based diet	315:361	Recently, we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility, arabinoxylan fermentability and broiler growth.
35505382	9	6	theme	digestive	1438:1446	arg1	tract					1448:1452	the digestive tract	1434:1452	the digestive tract	1434:1452	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	12	7	theme	cecal	1941:1945	arg1	communities					1947:1957	cecal communities	1941:1957	cecal communities	1941:1957	In contrast, both cereal type and enzyme supplementation were found to influence cecal communities.
35505382	14	8	theme	cereal	2114:2119	arg1	type					2121:2124	cereal type	2114:2124	cereal type	2114:2124	Further stratifying the analysis per cereal type revealed differences in microbiota composition between WC and WE, but not between MC and ME.
35505382	2	9	theme	nutrient	372:379	arg1	digestibility					381:393	nutrient digestibility	372:393	nutrient digestibility	372:393	Recently, we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility, arabinoxylan fermentability and broiler growth.
35505382	1	10	theme	non-starch	224:233	arg1	polysaccharides					235:249	non-starch polysaccharides	224:249	non-starch polysaccharides	224:249	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	10	11	theme	similar	1581:1587	arg1	diversity					1610:1618	A similar phylogenetic (alpha) diversity	1579:1618	A similar phylogenetic (alpha) diversity	1579:1618	A similar phylogenetic (alpha) diversity was observed among the four treatments, both in the ileal and the cecal samples.
35505382	11	12	theme	beta	1760:1763	arg1	ileum					1753:1757	the ileum	1749:1757	the ileum (beta diversity)	1749:1774	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	11	12	theme	beta	1760:1763	arg1	diversity					1765:1773	beta diversity	1760:1773	beta diversity	1760:1773	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	5	13	theme	microbial	923:931	arg1	communities					933:943	the microbial communities	919:943	the microbial communities in the ileum and ceca of broilers	919:977	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	0	14	from	composition	91:101	arg1	broilers					106:113	broilers	106:113	broilers	106:113	Cereal type and combined xylanase/glucanase supplementation influence the cecal microbiota composition in broilers.
35505382	5	15	theme	carbohydrate	805:816	arg1	fermentation					818:829	carbohydrate fermentation	805:829	carbohydrate fermentation in broilers	805:841	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	5	16	from	fermentation	818:829	arg1	broilers					834:841	broilers	834:841	broilers	834:841	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	4	17	from	present	662:668	arg1	diet					677:680	the diet	673:680	the diet	673:680	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	5	18	from	communities	933:943	arg1	ileum					952:956	ileum	952:956	ileum	952:956	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	5	18	from	communities	933:943	arg1	ceca					962:965	ceca	962:965	ceca	962:965	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	8	19	theme	finisher	1278:1285	arg1	diet					1287:1290	the wheat-based or maize-based finisher diet	1247:1290	the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME)	1247:1409	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	15	20	from	prevalence	2236:2245	arg1	ceca					2314:2317	the ceca	2310:2317	the ceca of broilers fed wheat-based diets	2310:2351	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	13	21	from	%	2014:2014	arg1	composition					2052:2062	microbial composition	2042:2062	microbial composition	2042:2062	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	13	21	from	%	2014:2014	arg1	ceca					2071:2074	the ceca	2067:2074	the ceca	2067:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	13	22	theme	total	2023:2027	arg1	variation					2029:2037	the total variation	2019:2037	the total variation in microbial composition in the ceca	2019:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	13	23	theme	variation	2029:2037	arg1	%					2014:2014	47%	2012:2014	47% of the total variation in microbial composition in the ceca	2012:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	13	23	theme	variation	2029:2037	arg1	variation					2029:2037	the total variation	2019:2037	the total variation in microbial composition in the ceca	2019:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	2	24	from	addition	334:341	arg1	diet					358:361	wheat-based diet	346:361	wheat-based diet	346:361	Recently, we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility, arabinoxylan fermentability and broiler growth.
35505382	6	25	theme	one-day-old	1001:1011	arg1	broilers					1018:1025	96 one-day-old male broilers	998:1025	96 one-day-old male broilers	998:1025	For that purpose, 96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.
35505382	11	26	theme	microbial	1724:1732	arg1	composition					1734:1744	a similar microbial composition	1714:1744	a similar microbial composition in the ileum (beta diversity)	1714:1774	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	9	27	theme	microbiota	1494:1503	arg1	composition					1505:1515	the ileal and cecal microbiota composition	1474:1515	the ileal and cecal microbiota composition	1474:1515	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	6	28	theme	maize-based	1095:1105	arg1	diets					1126:1130	either wheat-based or maize-based starter and grower diets	1073:1130	either wheat-based or maize-based starter and grower diets	1073:1130	For that purpose, 96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.
35505382	0	29	theme	cecal	74:78	arg1	composition					91:101	the cecal microbiota composition	70:101	the cecal microbiota composition in broilers	70:113	Cereal type and combined xylanase/glucanase supplementation influence the cecal microbiota composition in broilers.
35505382	5	30	theme	broilers	970:977	arg1	ileum					952:956	ileum	952:956	ileum	952:956	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	5	30	theme	broilers	970:977	arg1	ceca					962:965	ceca	962:965	ceca	962:965	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	6	31	theme	wheat-based	1080:1090	arg1	diets					1126:1130	either wheat-based or maize-based starter and grower diets	1073:1130	either wheat-based or maize-based starter and grower diets	1073:1130	For that purpose, 96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.
35505382	4	32	theme	digestion	594:602	arg1	improvement					570:580	enzyme-mediated improvement	554:580	enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation	554:632	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	8	33	theme	xylanase/glucanase	1352:1369	arg1	combination					1371:1381	a xylanase/glucanase combination	1350:1381	a xylanase/glucanase combination	1350:1381	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	12	34	theme	cereal	1878:1883	arg1	type					1885:1888	cereal type	1878:1888	cereal type	1878:1888	In contrast, both cereal type and enzyme supplementation were found to influence cecal communities.
35505382	15	35	dep	such	2269:2272	arg1	as					2274:2275	as	2274:2275	as	2274:2275	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	4	36	theme	carbohydrate	608:619	arg1	fermentation					621:632	carbohydrate fermentation	608:632	carbohydrate fermentation	608:632	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	1	37	theme	fiber-degrading	124:138	arg1	supplementation					147:161	Dietary fiber-degrading enzyme supplementation	116:161	Dietary fiber-degrading enzyme supplementation in broilers	116:173	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	9	38	theme	ribosomal	1539:1547	arg1	sequencing					1567:1576	ribosomal RNA gene amplicon sequencing	1539:1576	ribosomal RNA gene amplicon sequencing	1539:1576	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	16	39	theme	bacterial	2542:2550	arg1	metabolism					2552:2561	bacterial metabolism	2542:2561	bacterial metabolism	2542:2561	These findings indicated that fermentable arabinoxylan and arabinoxylo-oligosaccharides released by dietary xylanase may play an important role in bacterial metabolism.
35505382	4	40	theme	enzyme-mediated	554:568	arg1	improvement					570:580	enzyme-mediated improvement	554:580	enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation	554:632	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	9	41	theme	gene	1553:1556	arg1	sequencing					1567:1576	ribosomal RNA gene amplicon sequencing	1539:1576	ribosomal RNA gene amplicon sequencing	1539:1576	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	0	42	theme	Cereal	0:5	arg1	type					7:10	Cereal type	0:10	Cereal type	0:10	Cereal type and combined xylanase/glucanase supplementation influence the cecal microbiota composition in broilers.
35505382	8	43	dep	such	1295:1298	arg1	treatments					1309:1318	Control treatments	1301:1318	Control treatments	1301:1318	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	4	44	theme	type	657:660	arg1	present					662:668	the cereal type present	646:668	the cereal type present in the diet	646:680	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	1	45	dep	aims	175:178	arg1	off-setting					183:193	off-setting	183:193	off-setting the anti-nutritive effect of non-starch polysaccharides	183:249	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	1	45	dep	aims	175:178	arg1	promoting					258:266	promoting	258:266	promoting broiler health	258:281	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	8	46	dep	diet	1287:1290	arg1	such					1295:1298	such	1295:1298	such	1295:1298	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	8	46	dep	diet	1287:1290	arg1	supplemented					1332:1343	supplemented	1332:1343	supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME)	1332:1409	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	0	47	theme	xylanase/glucanase	25:42	arg1	supplementation					44:58	combined xylanase/glucanase supplementation	16:58	combined xylanase/glucanase supplementation	16:58	Cereal type and combined xylanase/glucanase supplementation influence the cecal microbiota composition in broilers.
35505382	5	48	theme	enzyme	870:875	arg1	supplementation					877:891	enzyme supplementation	870:891	enzyme supplementation	870:891	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	15	49	theme	Faecalibacterium	2277:2292	arg1	prevalence					2236:2245	the prevalence	2232:2245	the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets	2232:2351	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	11	50	theme	predominant	1818:1828	arg1	community					1830:1838	the predominant community	1814:1838	the predominant community for all treatments	1814:1857	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	11	50	theme	predominant	1818:1828	arg1	lactobacilli					1795:1806	lactobacilli	1795:1806	lactobacilli	1795:1806	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	8	51	dep	supplemented	1332:1343	arg1	treatments					1391:1400	Enzyme treatments	1384:1400	Enzyme treatments	1384:1400	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	8	51	dep	supplemented	1332:1343	arg1	ME					1407:1408	ME	1407:1408	ME	1407:1408	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	2	52	theme	arabinoxylan	396:407	arg1	fermentability					409:422	arabinoxylan fermentability	396:422	arabinoxylan fermentability	396:422	Recently, we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility, arabinoxylan fermentability and broiler growth.
35505382	1	53	theme	broiler	268:274	arg1	health					276:281	broiler health	268:281	broiler health	268:281	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	9	54	from	tract	1448:1452	arg1	samples					1421:1427	samples	1421:1427	samples from the digestive tract	1421:1452	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	10	55	dep	treatments	1648:1657	arg1	both					1660:1663	both	1660:1663	both	1660:1663	A similar phylogenetic (alpha) diversity was observed among the four treatments, both in the ileal and the cecal samples.
35505382	13	56	theme	cereal	1972:1977	arg1	type					1964:1967	The type	1960:1967	The type of cereal (i.e., wheat or maize)	1960:2000	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	14	57	theme	microbiota	2150:2159	arg1	composition					2161:2171	microbiota composition	2150:2171	microbiota composition	2150:2171	Further stratifying the analysis per cereal type revealed differences in microbiota composition between WC and WE, but not between MC and ME.
35505382	2	58	theme	wheat-based	346:356	arg1	diet					358:361	wheat-based diet	346:361	wheat-based diet	346:361	Recently, we demonstrated that xylanase/glucanase addition in wheat-based diet improved nutrient digestibility, arabinoxylan fermentability and broiler growth.
35505382	13	59	from	composition	2052:2062	arg1	%					2014:2014	47%	2012:2014	47% of the total variation in microbial composition in the ceca	2012:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	13	59	from	composition	2052:2062	arg1	variation					2029:2037	the total variation	2019:2037	the total variation in microbial composition in the ceca	2019:2074	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	1	60	theme	anti-nutritive	199:212	arg1	effect					214:219	the anti-nutritive effect	195:219	the anti-nutritive effect of non-starch polysaccharides	195:249	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	10	61	theme	alpha	1603:1607	arg1	diversity					1610:1618	A similar phylogenetic (alpha) diversity	1579:1618	A similar phylogenetic (alpha) diversity	1579:1618	A similar phylogenetic (alpha) diversity was observed among the four treatments, both in the ileal and the cecal samples.
35505382	10	62	theme	phylogenetic	1589:1600	arg1	diversity					1610:1618	A similar phylogenetic (alpha) diversity	1579:1618	A similar phylogenetic (alpha) diversity	1579:1618	A similar phylogenetic (alpha) diversity was observed among the four treatments, both in the ileal and the cecal samples.
35505382	5	63	theme	dietary	785:791	arg1	enzymes					793:799	dietary enzymes	785:799	dietary enzymes	785:799	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	1	64	theme	polysaccharides	235:249	arg1	effect					214:219	the anti-nutritive effect	195:219	the anti-nutritive effect of non-starch polysaccharides	195:249	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	8	65	theme	Control	1301:1307	arg1	treatments					1309:1318	Control treatments	1301:1318	Control treatments	1301:1318	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	8	66	theme	wheat-based	1251:1261	arg1	diet					1287:1290	the wheat-based or maize-based finisher diet	1247:1290	the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME)	1247:1409	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	9	67	theme	ileal	1478:1482	arg1	composition					1505:1515	the ileal and cecal microbiota composition	1474:1515	the ileal and cecal microbiota composition	1474:1515	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	8	68	theme	maize-based	1266:1276	arg1	diet					1287:1290	the wheat-based or maize-based finisher diet	1247:1290	the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME)	1247:1409	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	6	69	theme	male	1013:1016	arg1	broilers					1018:1025	96 one-day-old male broilers	998:1025	96 one-day-old male broilers	998:1025	For that purpose, 96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.
35505382	5	70	from	impact	860:865	arg1	diets					909:913	cereal-based diets	896:913	cereal-based diets	896:913	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	9	71	theme	cecal	1488:1492	arg1	composition					1505:1515	the ileal and cecal microbiota composition	1474:1515	the ileal and cecal microbiota composition	1474:1515	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	6	72	theme	starter	1107:1113	arg1	diets					1126:1130	either wheat-based or maize-based starter and grower diets	1073:1130	either wheat-based or maize-based starter and grower diets	1073:1130	For that purpose, 96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.
35505382	11	73	from	composition	1734:1744	arg1	ileum					1753:1757	the ileum	1749:1757	the ileum (beta diversity)	1749:1774	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	11	73	from	composition	1734:1744	arg1	diversity					1765:1773	beta diversity	1760:1773	beta diversity	1760:1773	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	0	74	theme	microbiota	80:89	arg1	composition					91:101	the cecal microbiota composition	70:101	the cecal microbiota composition in broilers	70:113	Cereal type and combined xylanase/glucanase supplementation influence the cecal microbiota composition in broilers.
35505382	13	75	theme	microbial	2042:2050	arg1	composition					2052:2062	microbial composition	2042:2062	microbial composition	2042:2062	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	8	76	dep	treatments	1391:1400	arg1	WE					1403:1404	WE	1403:1404	WE	1403:1404	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	16	77	theme	important	2524:2532	arg1	role					2534:2537	an important role	2521:2537	an important role	2521:2537	These findings indicated that fermentable arabinoxylan and arabinoxylo-oligosaccharides released by dietary xylanase may play an important role in bacterial metabolism.
35505382	15	78	theme	beneficial	2250:2259	arg1	genera					2261:2266	beneficial genera	2250:2266	beneficial genera	2250:2266	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	11	79	theme	similar	1716:1722	arg1	composition					1734:1744	a similar microbial composition	1714:1744	a similar microbial composition in the ileum (beta diversity)	1714:1774	Furthermore, a similar microbial composition in the ileum (beta diversity) was observed, with lactobacilli being the predominant community for all treatments.
35505382	15	80	theme	arabinoxylan	2368:2379	arg1	accumulation					2381:2392	arabinoxylan accumulation	2368:2392	arabinoxylan accumulation	2368:2392	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	14	81	from	differences	2135:2145	arg1	composition					2161:2171	microbiota composition	2150:2171	microbiota composition	2150:2171	Further stratifying the analysis per cereal type revealed differences in microbiota composition between WC and WE, but not between MC and ME.
35505382	4	82	theme	fermentation	621:632	arg1	improvement					570:580	enzyme-mediated improvement	554:580	enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation	554:632	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	15	83	theme	wheat-based	2335:2345	arg1	diets					2347:2351	wheat-based diets	2335:2351	wheat-based diets	2335:2351	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	12	84	theme	enzyme	1894:1899	arg1	supplementation					1901:1915	enzyme supplementation	1894:1915	enzyme supplementation	1894:1915	In contrast, both cereal type and enzyme supplementation were found to influence cecal communities.
35505382	15	85	theme	broilers	2322:2329	arg1	ceca					2314:2317	the ceca	2310:2317	the ceca of broilers fed wheat-based diets	2310:2351	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	8	86	dep	treatments	1309:1318	arg1	MC					1325:1326	MC	1325:1326	MC	1325:1326	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	8	86	dep	treatments	1309:1318	arg1	WC					1321:1322	WC	1321:1322	WC	1321:1322	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	13	87	dep	wheat	1986:1990	arg1	i.e.					1980:1983	i.e.	1980:1983	i.e.	1980:1983	The type of cereal (i.e., wheat or maize) explained 47% of the total variation in microbial composition in the ceca.
35505382	4	88	theme	nutrient	585:592	arg1	digestion					594:602	nutrient digestion	585:602	nutrient digestion	585:602	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	8	89	theme	Enzyme	1384:1389	arg1	treatments					1391:1400	Enzyme treatments	1384:1400	Enzyme treatments	1384:1400	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	8	89	theme	Enzyme	1384:1389	arg1	ME					1407:1408	ME	1407:1408	ME	1407:1408	The broilers received for 8 d the wheat-based or maize-based finisher diet as such (Control treatments; WC, MC) or supplemented with a xylanase/glucanase combination (Enzyme treatments; WE, ME).
35505382	5	90	theme	cereal-based	896:907	arg1	diets					909:913	cereal-based diets	896:913	cereal-based diets	896:913	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	9	91	theme	amplicon	1558:1565	arg1	sequencing					1567:1576	ribosomal RNA gene amplicon sequencing	1539:1576	ribosomal RNA gene amplicon sequencing	1539:1576	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	10	92	dep	ileal	1672:1676	arg1	samples					1692:1698	samples	1692:1698	samples	1692:1698	A similar phylogenetic (alpha) diversity was observed among the four treatments, both in the ileal and the cecal samples.
35505382	3	93	theme	xylanase	507:514	arg1	action					516:521	xylanase action	507:521	xylanase action	507:521	Conversely, maize arabinoxylan was found to be recalcitrant to xylanase action.
35505382	0	94	theme	combined	16:23	arg1	supplementation					44:58	combined xylanase/glucanase supplementation	16:58	combined xylanase/glucanase supplementation	16:58	Cereal type and combined xylanase/glucanase supplementation influence the cecal microbiota composition in broilers.
35505382	12	95	located	found	1922:1926	arg2	supplementation					1901:1915	enzyme supplementation	1894:1915	enzyme supplementation	1894:1915	In contrast, both cereal type and enzyme supplementation were found to influence cecal communities.
35505382	12	95	located	found	1922:1926	arg2	type					1885:1888	cereal type	1878:1888	cereal type	1878:1888	In contrast, both cereal type and enzyme supplementation were found to influence cecal communities.
35505382	12	95	located	found	1922:1926	arg1	contrast					1863:1870	contrast	1863:1870	contrast	1863:1870	In contrast, both cereal type and enzyme supplementation were found to influence cecal communities.
35505382	1	96	from	supplementation	147:161	arg1	broilers					166:173	broilers	166:173	broilers	166:173	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	1	97	theme	Dietary	116:122	arg1	supplementation					147:161	Dietary fiber-degrading enzyme supplementation	116:161	Dietary fiber-degrading enzyme supplementation in broilers	116:173	Dietary fiber-degrading enzyme supplementation in broilers aims at off-setting the anti-nutritive effect of non-starch polysaccharides and at promoting broiler health.
35505382	3	98	theme	maize	456:460	arg1	arabinoxylan					462:473	maize arabinoxylan	456:473	maize arabinoxylan	456:473	Conversely, maize arabinoxylan was found to be recalcitrant to xylanase action.
35505382	9	99	theme	RNA	1549:1551	arg1	sequencing					1567:1576	ribosomal RNA gene amplicon sequencing	1539:1576	ribosomal RNA gene amplicon sequencing	1539:1576	At d 28, samples from the digestive tract were collected, and the ileal and cecal microbiota composition was determined by 16S ribosomal RNA gene amplicon sequencing.
35505382	7	100	theme	dietary	1197:1203	arg1	treatments					1205:1214	one out of four dietary treatments	1181:1214	one out of four dietary treatments	1181:1214	At d 20, the broilers were randomly assigned to one out of four dietary treatments.
35505382	16	101	theme	fermentable	2425:2435	arg1	arabinoxylan					2437:2448	fermentable arabinoxylan	2425:2448	fermentable arabinoxylan	2425:2448	These findings indicated that fermentable arabinoxylan and arabinoxylo-oligosaccharides released by dietary xylanase may play an important role in bacterial metabolism.
35505382	6	102	theme	grower	1119:1124	arg1	diets					1126:1130	either wheat-based or maize-based starter and grower diets	1073:1130	either wheat-based or maize-based starter and grower diets	1073:1130	For that purpose, 96 one-day-old male broilers were randomly reared in two pens and received either wheat-based or maize-based starter and grower diets.
35505382	5	103	theme	supplementation	877:891	arg1	impact					860:865	the impact	856:865	the impact of enzyme supplementation in cereal-based diets	856:913	Hence, we aimed at further investigating the link between dietary enzymes and carbohydrate fermentation in broilers, by studying the impact of enzyme supplementation in cereal-based diets, to the microbial communities in the ileum and ceca of broilers.
35505382	4	104	theme	cereal	650:655	arg1	present					662:668	the cereal type present	646:668	the cereal type present in the diet	646:680	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
35505382	15	105	theme	genera	2261:2266	arg1	prevalence					2236:2245	the prevalence	2232:2245	the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets	2232:2351	Furthermore, the prevalence of beneficial genera, such as Faecalibacterium and Blautia, in the ceca of broilers fed wheat-based diets coincided with arabinoxylan accumulation.
35505382	16	106	theme	dietary	2495:2501	arg1	xylanase					2503:2510	dietary xylanase	2495:2510	dietary xylanase	2495:2510	These findings indicated that fermentable arabinoxylan and arabinoxylo-oligosaccharides released by dietary xylanase may play an important role in bacterial metabolism.
35505382	4	107	theme	broiler	711:717	arg1	growth					719:724	broiler growth	711:724	broiler growth	711:724	These findings suggested that enzyme-mediated improvement of nutrient digestion and carbohydrate fermentation depended on the cereal type present in the diet, and may have contributed to broiler growth.
36816663	1	0	used	used	184:187	arg2	they					172:175	they	172:175	they	172:175	Natural polysaccharides are biocompatible and biodegradable; therefore, they can be used as feedstock for biodegradable structural materials and biomaterials.
36816663	1	0	used	used	184:187	arg2	feedstock					192:200	feedstock	192:200	feedstock	192:200	Natural polysaccharides are biocompatible and biodegradable; therefore, they can be used as feedstock for biodegradable structural materials and biomaterials.
36816663	7	1	used	used	1299:1302	arg2	films					1286:1290	these films	1280:1290	these films	1280:1290	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	7	1	used	used	1299:1302	arg2	materials					1320:1328	shape-memory materials	1307:1328	shape-memory materials	1307:1328	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	2	2	theme	chondroitin	331:341	arg1	C					351:351	chondroitin sulfate C	331:351	chondroitin sulfate C (CS)	331:356	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	2	2	theme	chondroitin	331:341	arg1	CS					354:355	CS	354:355	CS	354:355	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	4	3	theme	films	730:734	arg1	formability					711:721	the formability	707:721	the formability of the films	707:734	The roll-press conditions were optimized, and it was observed that the molecular weight of CHI did not significantly affect the formability of the films, whereas the roll temperature and rolling speed were important.
36816663	4	4	theme	roll-press	587:596	arg1	conditions					598:607	The roll-press conditions	583:607	The roll-press conditions	583:607	The roll-press conditions were optimized, and it was observed that the molecular weight of CHI did not significantly affect the formability of the films, whereas the roll temperature and rolling speed were important.
36816663	6	5	theme	films	1110:1114	arg1	shrank					1116:1121	the films shrank	1106:1121	the films shrank in the MD	1106:1131	Additionally, the films shrank in the MD and expanded in the TD after immersion in aqueous solutions, followed by drying.
36816663	3	6	theme	composite	533:541	arg1	films					543:547	the composite films	529:547	the composite films prepared by hot-press techniques	529:580	The obtained films (CS/CHI films) were thin and transparent, similar to the composite films prepared by hot-press techniques.
36816663	5	7	theme	films	838:842	arg1	tests					812:816	The tensile tests	800:816	The tensile tests of the roll-pressed films	800:842	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	8	theme	mechanical	1056:1065	arg1	anisotropy					1067:1076	mechanical anisotropy	1056:1076	mechanical anisotropy	1056:1076	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	9	from	strength	873:880	arg1	MD					924:925	MD	924:925	MD	924:925	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	9	from	strength	873:880	arg1	direction					913:921	the mechanical direction	898:921	the mechanical direction (MD)	898:926	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	10	theme	5	946:946	arg1	strength					873:880	the mechanical strength	858:880	the mechanical strength of the films in the mechanical direction (MD)	858:926	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	10	theme	5	946:946	arg1	times					948:952	times	948:952	times	948:952	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	2	11	theme	complex	412:418	arg1	gels					426:429	their polyion complex (PIC) gels	398:429	their polyion complex (PIC) gels	398:429	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	1	12	theme	Natural	100:106	arg1	polysaccharides					108:122	Natural polysaccharides	100:122	Natural polysaccharides	100:122	Natural polysaccharides are biocompatible and biodegradable; therefore, they can be used as feedstock for biodegradable structural materials and biomaterials.
36816663	2	13	theme	polyion	404:410	arg1	PIC					421:423	PIC	421:423	PIC	421:423	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	2	13	theme	polyion	404:410	arg1	complex					412:418	their polyion complex	398:418	their polyion complex (PIC) gels	398:429	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	3	14	theme	hot-press	561:569	arg1	techniques					571:580	hot-press techniques	561:580	hot-press techniques	561:580	The obtained films (CS/CHI films) were thin and transparent, similar to the composite films prepared by hot-press techniques.
36816663	1	15	theme	biodegradable	206:218	arg1	materials					231:239	biodegradable structural materials	206:239	biodegradable structural materials	206:239	Natural polysaccharides are biocompatible and biodegradable; therefore, they can be used as feedstock for biodegradable structural materials and biomaterials.
36816663	2	16	theme	roll-press	434:443	arg1	techniques					445:454	roll-press techniques	434:454	roll-press techniques	434:454	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	1	17	theme	structural	220:229	arg1	materials					231:239	biodegradable structural materials	206:239	biodegradable structural materials	206:239	Natural polysaccharides are biocompatible and biodegradable; therefore, they can be used as feedstock for biodegradable structural materials and biomaterials.
36816663	0	18	theme	Mechanically	15:26	arg1	Films					65:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Preparation of Mechanically Anisotropic Polysaccharide Composite Films Using Roll-Press Techniques.
36816663	2	19	theme	sulfate	343:349	arg1	C					351:351	chondroitin sulfate C	331:351	chondroitin sulfate C (CS)	331:356	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	2	19	theme	sulfate	343:349	arg1	CS					354:355	CS	354:355	CS	354:355	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	6	20	from	shrank	1116:1121	arg1	MD					1130:1131	the MD	1126:1131	the MD	1126:1131	Additionally, the films shrank in the MD and expanded in the TD after immersion in aqueous solutions, followed by drying.
36816663	5	21	theme	mechanical	862:871	arg1	strength					873:880	the mechanical strength	858:880	the mechanical strength of the films in the mechanical direction (MD)	858:926	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	21	theme	mechanical	862:871	arg1	times					948:952	times	948:952	times	948:952	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	0	22	theme	Composite	55:63	arg1	Films					65:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Preparation of Mechanically Anisotropic Polysaccharide Composite Films Using Roll-Press Techniques.
36816663	7	23	theme	anisotropic	1219:1229	arg1	properties					1255:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	6	24	theme	aqueous	1175:1181	arg1	solutions					1183:1191	aqueous solutions	1175:1191	aqueous solutions	1175:1191	Additionally, the films shrank in the MD and expanded in the TD after immersion in aqueous solutions, followed by drying.
36816663	4	25	theme	molecular	654:662	arg1	weight					664:669	the molecular weight	650:669	the molecular weight of CHI	650:676	The roll-press conditions were optimized, and it was observed that the molecular weight of CHI did not significantly affect the formability of the films, whereas the roll temperature and rolling speed were important.
36816663	7	26	theme	shrinking	1231:1239	arg1	properties					1255:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	0	27	theme	Polysaccharide	40:53	arg1	Films					65:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Preparation of Mechanically Anisotropic Polysaccharide Composite Films Using Roll-Press Techniques.
36816663	5	28	theme	roll-press	1025:1034	arg1	techniques					1036:1045	the roll-press techniques	1021:1045	the roll-press techniques	1021:1045	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	0	29	theme	Anisotropic	28:38	arg1	Films					65:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Mechanically Anisotropic Polysaccharide Composite Films	15:69	Preparation of Mechanically Anisotropic Polysaccharide Composite Films Using Roll-Press Techniques.
36816663	3	30	theme	obtained	461:468	arg1	films					470:474	The obtained films	457:474	The obtained films (CS/CHI films)	457:489	The obtained films (CS/CHI films) were thin and transparent, similar to the composite films prepared by hot-press techniques.
36816663	3	30	theme	obtained	461:468	arg1	films					484:488	CS/CHI films	477:488	CS/CHI films	477:488	The obtained films (CS/CHI films) were thin and transparent, similar to the composite films prepared by hot-press techniques.
36816663	3	30	theme	obtained	461:468	arg1	thin					496:499	thin	496:499	thin	496:499	The obtained films (CS/CHI films) were thin and transparent, similar to the composite films prepared by hot-press techniques.
36816663	7	31	theme	shape-memory	1307:1318	arg1	materials					1320:1328	shape-memory materials	1307:1328	shape-memory materials	1307:1328	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	7	31	theme	shape-memory	1307:1318	arg1	films					1286:1290	these films	1280:1290	these films	1280:1290	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	5	32	theme	films	889:893	arg1	strength					873:880	the mechanical strength	858:880	the mechanical strength of the films in the mechanical direction (MD)	858:926	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	32	theme	films	889:893	arg1	times					948:952	times	948:952	times	948:952	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	0	33	theme	Films	65:69	arg1	Preparation					0:10	Preparation	0:10	Preparation of Mechanically Anisotropic Polysaccharide Composite Films Using Roll-Press Techniques.	0:98	Preparation of Mechanically Anisotropic Polysaccharide Composite Films Using Roll-Press Techniques.
36816663	7	34	theme	Such	1214:1217	arg1	properties					1255:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	5	35	dep	times	948:952	arg1	higher					954:959	higher	954:959	higher	954:959	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	6	36	from	immersion	1162:1170	arg1	solutions					1183:1191	aqueous solutions	1175:1191	aqueous solutions	1175:1191	Additionally, the films shrank in the MD and expanded in the TD after immersion in aqueous solutions, followed by drying.
36816663	4	37	theme	rolling	770:776	arg1	speed					778:782	rolling speed	770:782	rolling speed	770:782	The roll-press conditions were optimized, and it was observed that the molecular weight of CHI did not significantly affect the formability of the films, whereas the roll temperature and rolling speed were important.
36816663	2	38	theme	composite	301:309	arg1	films					311:315	anisotropic polysaccharide composite films	274:315	anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI)	274:375	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	5	39	theme	transverse	978:987	arg1	TD					1000:1001	TD	1000:1001	TD	1000:1001	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	39	theme	transverse	978:987	arg1	direction					989:997	the transverse direction	974:997	the transverse direction (TD)	974:1002	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	3	40	theme	CS/CHI	477:482	arg1	films					470:474	The obtained films	457:474	The obtained films (CS/CHI films)	457:489	The obtained films (CS/CHI films) were thin and transparent, similar to the composite films prepared by hot-press techniques.
36816663	3	40	theme	CS/CHI	477:482	arg1	films					484:488	CS/CHI films	477:488	CS/CHI films	477:488	The obtained films (CS/CHI films) were thin and transparent, similar to the composite films prepared by hot-press techniques.
36816663	4	41	theme	roll	749:752	arg1	important					789:797	important	789:797	important	789:797	The roll-press conditions were optimized, and it was observed that the molecular weight of CHI did not significantly affect the formability of the films, whereas the roll temperature and rolling speed were important.
36816663	4	41	theme	roll	749:752	arg1	temperature					754:764	the roll temperature	745:764	the roll temperature	745:764	The roll-press conditions were optimized, and it was observed that the molecular weight of CHI did not significantly affect the formability of the films, whereas the roll temperature and rolling speed were important.
36816663	2	42	theme	polysaccharide	286:299	arg1	films					311:315	anisotropic polysaccharide composite films	274:315	anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI)	274:375	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	5	43	theme	mechanical	902:911	arg1	MD					924:925	MD	924:925	MD	924:925	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	43	theme	mechanical	902:911	arg1	direction					913:921	the mechanical direction	898:921	the mechanical direction (MD)	898:926	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	5	44	theme	roll-pressed	825:836	arg1	films					838:842	the roll-pressed films	821:842	the roll-pressed films	821:842	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36816663	2	45	theme	anisotropic	274:284	arg1	films					311:315	anisotropic polysaccharide composite films	274:315	anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI)	274:375	In this study, anisotropic polysaccharide composite films consisting of chondroitin sulfate C (CS) and chitosan (CHI) were fabricated from their polyion complex (PIC) gels by roll-press techniques.
36816663	7	46	theme	expanding	1245:1253	arg1	properties					1255:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties	1214:1264	Such anisotropic shrinking and expanding properties indicate that these films can be used as shape-memory materials.
36816663	4	47	theme	CHI	674:676	arg1	weight					664:669	the molecular weight	650:669	the molecular weight of CHI	650:676	The roll-press conditions were optimized, and it was observed that the molecular weight of CHI did not significantly affect the formability of the films, whereas the roll temperature and rolling speed were important.
36816663	0	48	theme	Roll-Press	77:86	arg1	Techniques					88:97	Roll-Press Techniques	77:97	Roll-Press Techniques	77:97	Preparation of Mechanically Anisotropic Polysaccharide Composite Films Using Roll-Press Techniques.
36816663	5	49	theme	tensile	804:810	arg1	tests					812:816	The tensile tests	800:816	The tensile tests of the roll-pressed films	800:842	The tensile tests of the roll-pressed films revealed that the mechanical strength of the films in the mechanical direction (MD) was approximately 5 times higher than that in the transverse direction (TD), indicating that the roll-press techniques imparted mechanical anisotropy to the films.
36076885	6	0	theme	AAP	1422:1424	arg1	related					1433:1439	related	1433:1439	related	1433:1439	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	6	0	theme	AAP	1422:1424	arg1	bioactivities					1405:1417	The bioactivities	1401:1417	The bioactivities of AAP	1401:1424	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	5	1	theme	cibaria	1192:1198	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	2	2	theme	nutritional	587:597	arg1	food					599:602	nutritional food	587:602	nutritional food with Auricularia auricula polysaccharides (AAP)	587:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	3	3	theme	metabolomic	704:714	arg1	results					716:722	LC-MS/MS metabolomic results	695:722	LC-MS/MS metabolomic results	695:722	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	0	4	from	Microbiota	55:64	arg1	Mice					93:96	Mice	93:96	Mice	93:96	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	5	5	theme	relative	1128:1135	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	3	6	theme	sphingolipid	987:998	arg1	pathway					1011:1017	the sphingolipid metabolism pathway	983:1017	the sphingolipid metabolism pathway	983:1017	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	5	7	theme	uniformis	1284:1292	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	5	8	theme	cowanii	1211:1217	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	0	9	from	Effects	0:6	arg1	Phenotype					80:88	Metabolic Phenotype	70:88	Metabolic Phenotype	70:88	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	0	9	from	Effects	0:6	arg1	Microbiota					55:64	Gut Microbiota	51:64	Gut Microbiota	51:64	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	3	10	theme	metabolism	1000:1009	arg1	pathway					1011:1017	the sphingolipid metabolism pathway	983:1017	the sphingolipid metabolism pathway	983:1017	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	4	11	theme	AAP	1052:1054	arg1	supplementation					1033:1047	supplementation	1033:1047	supplementation of AAP	1033:1054	Furthermore, supplementation of AAP significantly changed the composition of the mice intestinal flora.
36076885	3	12	theme	following	871:879	arg1	glycine					916:922	glycine	916:922	glycine	916:922	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	12	theme	following	871:879	arg1	glycerophospholipid					951:969	glycerophospholipid	951:969	glycerophospholipid	951:969	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	12	theme	following	871:879	arg1	metabolisms					881:891	the following metabolisms	867:891	the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid	867:969	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	12	theme	following	871:879	arg1	arginine					894:901	arginine	894:901	arginine	894:901	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	12	theme	following	871:879	arg1	serine					925:930	serine	925:930	serine	925:930	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	12	theme	following	871:879	arg1	threonine					936:944	threonine	936:944	threonine	936:944	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	12	theme	following	871:879	arg1	proline					907:913	proline	907:913	proline	907:913	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	1	13	theme	overall	332:338	arg1	metabolism					340:349	overall metabolism	332:349	overall metabolism	332:349	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	5	14	theme	Firmicutes	1354:1363	arg1	M10-2					1375:1379	Firmicutes bacterium M10-2	1354:1379	Firmicutes bacterium M10-2	1354:1379	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	1	15	from	microbiota	359:368	arg1	mice					389:392	normal C57BL/6J mice	373:392	normal C57BL/6J mice	373:392	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	2	16	theme	auricula	621:628	arg1	AAP					647:649	AAP	647:649	AAP	647:649	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	16	theme	auricula	621:628	arg1	polysaccharides					630:644	Auricularia auricula polysaccharides	609:644	Auricularia auricula polysaccharides (AAP)	609:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	3	17	theme	biosynthesis	798:809	arg1	pathway					811:817	the arginine biosynthesis pathway	785:817	the arginine biosynthesis pathway	785:817	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	5	18	theme	bacterium	1365:1373	arg1	M10-2					1375:1379	Firmicutes bacterium M10-2	1354:1379	Firmicutes bacterium M10-2	1354:1379	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	2	19	dep	Auricularia	526:536	arg1	auricula					538:545	auricula	538:545	auricula	538:545	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	3	20	theme	LC-MS/MS	695:702	arg1	results					716:722	LC-MS/MS metabolomic results	695:722	LC-MS/MS metabolomic results	695:722	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	1	21	from	effects	262:268	arg1	metabolism					340:349	overall metabolism	332:349	overall metabolism	332:349	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	1	21	from	effects	262:268	arg1	microbiota					359:368	gut microbiota	355:368	gut microbiota	355:368	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	2	22	theme	functional	664:673	arg1	component					675:683	the main functional component	655:683	the main functional component	655:683	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	22	theme	functional	664:673	arg1	basis					485:489	a scientific basis	472:489	a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP)	472:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	23	from	use	519:521	arg1	Mountains					570:578	Mountains	570:578	Mountains	570:578	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	23	from	use	519:521	arg1	food					599:602	nutritional food	587:602	nutritional food with Auricularia auricula polysaccharides (AAP)	587:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	5	24	theme	M10-2	1375:1379	arg1	abundance					1341:1349	the relative abundance	1328:1349	the relative abundance of Firmicutes bacterium M10-2	1328:1379	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	2	25	theme	further	495:501	arg1	development					503:513	further development	495:513	further development	495:513	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	26	theme	metabolomic	425:435	arg1	methods					453:459	metabolomic and microbiomic methods	425:459	metabolomic and microbiomic methods	425:459	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	1	27	theme	gut	355:357	arg1	microbiota					359:368	gut microbiota	355:368	gut microbiota	355:368	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	6	28	theme	microbiota	1500:1509	arg1	composition					1511:1521	gut microbiota composition	1496:1521	gut microbiota composition	1496:1521	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	2	29	theme	main	659:662	arg1	component					675:683	the main functional component	655:683	the main functional component	655:683	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	29	theme	main	659:662	arg1	basis					485:489	a scientific basis	472:489	a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP)	472:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	3	30	dep	found	759:763	arg1	enriched					773:780	enriched	773:780	enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway	773:1017	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	6	31	theme	endogenous	1470:1479	arg1	metabolism					1481:1490	endogenous metabolism	1470:1490	endogenous metabolism	1470:1490	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	6	32	theme	gut	1496:1498	arg1	composition					1511:1521	gut microbiota composition	1496:1521	gut microbiota composition	1496:1521	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	4	33	theme	mice	1101:1104	arg1	flora					1117:1121	the mice intestinal flora	1097:1121	the mice intestinal flora	1097:1121	Furthermore, supplementation of AAP significantly changed the composition of the mice intestinal flora.
36076885	0	34	theme	auricula	23:30	arg1	Polysaccharides					32:46	Auricularia auricula Polysaccharides	11:46	Auricularia auricula Polysaccharides	11:46	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	1	35	theme	Personalized	99:110	arg1	diets					112:116	Personalized diets	99:116	Personalized diets	99:116	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	2	36	with	food	599:602	arg1	AAP					647:649	AAP	647:649	AAP	647:649	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	36	with	food	599:602	arg1	polysaccharides					630:644	Auricularia auricula polysaccharides	609:644	Auricularia auricula polysaccharides (AAP)	609:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	1	37	from	metabolism	340:349	arg1	mice					389:392	normal C57BL/6J mice	373:392	normal C57BL/6J mice	373:392	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	1	38	theme	regulatory	251:260	arg1	effects					262:268	the regulatory effects	247:268	the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice	247:392	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	2	39	theme	Auricularia	526:536	arg1	resources					547:555	Auricularia auricula resources	526:555	Auricularia auricula resources	526:555	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	4	40	theme	intestinal	1106:1115	arg1	flora					1117:1121	the mice intestinal flora	1097:1121	the mice intestinal flora	1097:1121	Furthermore, supplementation of AAP significantly changed the composition of the mice intestinal flora.
36076885	4	41	theme	flora	1117:1121	arg1	composition					1082:1092	the composition	1078:1092	the composition of the mice intestinal flora	1078:1121	Furthermore, supplementation of AAP significantly changed the composition of the mice intestinal flora.
36076885	6	42	theme	regulatory	1448:1457	arg1	effects					1459:1465	the regulatory effects	1444:1465	the regulatory effects of endogenous metabolism and gut microbiota composition	1444:1521	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	3	43	theme	AAP-regulated	728:740	arg1	metabolites					742:752	51 AAP-regulated metabolites	725:752	51 AAP-regulated metabolites	725:752	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	0	44	from	Phenotype	80:88	arg1	Mice					93:96	Mice	93:96	Mice	93:96	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	6	45	theme	metabolism	1481:1490	arg1	effects					1459:1465	the regulatory effects	1444:1465	the regulatory effects of endogenous metabolism and gut microbiota composition	1444:1521	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	3	46	dep	along	972:976	arg1	with					978:981	with	978:981	with	978:981	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	0	47	theme	Polysaccharides	32:46	arg1	Effects					0:6	Effects	0:6	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.	0:97	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	5	48	theme	faecalis	1233:1240	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	1	49	theme	normal	373:378	arg1	mice					389:392	normal C57BL/6J mice	373:392	normal C57BL/6J mice	373:392	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	3	50	dep	metabolisms	881:891	arg1	arginine					894:901	arginine	894:901	arginine	894:901	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	50	dep	metabolisms	881:891	arg1	glycerophospholipid					951:969	glycerophospholipid	951:969	glycerophospholipid	951:969	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	50	dep	metabolisms	881:891	arg1	metabolisms					881:891	the following metabolisms	867:891	the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid	867:969	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	50	dep	metabolisms	881:891	arg1	glycine					916:922	glycine	916:922	glycine	916:922	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	50	dep	metabolisms	881:891	arg1	proline					907:913	proline	907:913	proline	907:913	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	5	51	theme	johnsonii	1171:1179	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	2	52	theme	microbiomic	441:451	arg1	methods					453:459	metabolomic and microbiomic methods	425:459	metabolomic and microbiomic methods	425:459	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	1	53	theme	polysaccharides	273:287	arg1	effects					262:268	the regulatory effects	247:268	the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice	247:392	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	1	54	theme	C57BL/6J	380:387	arg1	mice					389:392	normal C57BL/6J mice	373:392	normal C57BL/6J mice	373:392	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	3	55	contain	had	826:828	arg1	pathway					1011:1017	the sphingolipid metabolism pathway	983:1017	the sphingolipid metabolism pathway	983:1017	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	55	contain	had	826:828	arg1	pathway					811:817	the arginine biosynthesis pathway	785:817	the arginine biosynthesis pathway	785:817	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	3	55	contain	had	826:828	arg2	correlation					842:852	the highest correlation	830:852	the highest correlation	830:852	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	0	56	theme	Gut	51:53	arg1	Microbiota					55:64	Gut Microbiota	51:64	Gut Microbiota	51:64	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	2	57	theme	Auricularia	609:619	arg1	AAP					647:649	AAP	647:649	AAP	647:649	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	57	theme	Auricularia	609:619	arg1	polysaccharides					630:644	Auricularia auricula polysaccharides	609:644	Auricularia auricula polysaccharides (AAP)	609:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	1	58	dep	metabolism	340:349	arg1	the					328:330	the	328:330	the	328:330	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	1	59	theme	internal	129:136	arg1	metabolism					138:147	the internal metabolism	125:147	the internal metabolism	125:147	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	3	60	theme	arginine	789:796	arg1	pathway					811:817	the arginine biosynthesis pathway	785:817	the arginine biosynthesis pathway	785:817	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	2	61	theme	resources	547:555	arg1	use					519:521	use	519:521	use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP)	519:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	61	theme	resources	547:555	arg1	development					503:513	further development	495:513	further development	495:513	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	5	62	theme	relative	1332:1339	arg1	abundance					1341:1349	the relative abundance	1328:1349	the relative abundance of Firmicutes bacterium M10-2	1328:1379	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	1	63	theme	body	205:208	arg1	health					191:196	the health	187:196	the health of the body	187:208	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	0	64	theme	Metabolic	70:78	arg1	Phenotype					80:88	Metabolic Phenotype	70:88	Metabolic Phenotype	70:88	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	2	65	from	development	503:513	arg1	Mountains					570:578	Mountains	570:578	Mountains	570:578	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	65	from	development	503:513	arg1	food					599:602	nutritional food	587:602	nutritional food with Auricularia auricula polysaccharides (AAP)	587:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	5	66	theme	abundance	1137:1145	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36076885	3	67	theme	highest	834:840	arg1	correlation					842:852	the highest correlation	830:852	the highest correlation	830:852	Based on LC-MS/MS metabolomic results, 51 AAP-regulated metabolites were found, mainly enriched in the arginine biosynthesis pathway, which had the highest correlation, followed by the following metabolisms: arginine and proline; glycine, serine and threonine; and glycerophospholipid, along with the sphingolipid metabolism pathway.
36076885	2	68	theme	scientific	474:483	arg1	component					675:683	the main functional component	655:683	the main functional component	655:683	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	2	68	theme	scientific	474:483	arg1	basis					485:489	a scientific basis	472:489	a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP)	472:650	The study was conducted using metabolomic and microbiomic methods to provide a scientific basis for further development and use of Auricularia auricula resources in the Qinba Mountains and in nutritional food with Auricularia auricula polysaccharides (AAP) as the main functional component.
36076885	0	69	theme	Auricularia	11:21	arg1	Polysaccharides					32:46	Auricularia auricula Polysaccharides	11:46	Auricularia auricula Polysaccharides	11:46	Effects of Auricularia auricula Polysaccharides on Gut Microbiota and Metabolic Phenotype in Mice.
36076885	1	70	theme	organisms	152:160	arg1	metabolism					138:147	the internal metabolism	125:147	the internal metabolism	125:147	Personalized diets change the internal metabolism of organisms, which, in turn, affects the health of the body; this study was performed to explore the regulatory effects of polysaccharides extracted from Auricularia auricula on the overall metabolism and gut microbiota in normal C57BL/6J mice.
36076885	6	71	theme	composition	1511:1521	arg1	effects					1459:1465	the regulatory effects	1444:1465	the regulatory effects of endogenous metabolism and gut microbiota composition	1444:1521	The bioactivities of AAP may be related to the regulatory effects of endogenous metabolism and gut microbiota composition.
36076885	5	72	theme	animalis	1259:1266	arg1	levels					1147:1152	The relative abundance levels	1124:1152	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis	1124:1292	The relative abundance levels of Lactobacillus johnsonii, Weissella cibaria, Kosakonia cowanii, Enterococcus faecalis, Bifidobacterium animalis and Bacteroides uniformis were markedly up-regulated, while the relative abundance of Firmicutes bacterium M10-2 was down-regulated.
36433011	0	0	theme	Okra	93:96	arg1	Waste					104:108	Okra Plant Waste	93:108	Okra Plant Waste for Food Packaging Application	93:139	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	8	1	theme	plant	1616:1620	arg1	residues					1622:1629	okra plant residues	1611:1629	okra plant residues for edible food packaging	1611:1655	This study successfully applied value-added polysaccharides derived from okra plant residues for edible food packaging.
36433011	4	2	contain	containing	937:946	arg1	films					931:935	OLP/CMC films	923:935	OLP/CMC films containing up to 10% OLP	923:960	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	4	2	contain	containing	937:946	arg2	OLP					958:960	up to 10% OLP	948:960	up to 10% OLP	948:960	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	4	3	theme	oxygen	864:869	arg1	permeability					899:910	and oxygen (39%) and water vapor (32%) permeability	860:910	and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP	860:960	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	3	4	from	differences	581:591	arg1	behavior					608:615	rheological behavior	596:615	rheological behavior (n, 0.478-0.743)	596:632	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	6	5	theme	high	1207:1210	arg1	loss					1219:1222	high weight loss	1207:1222	high weight loss (22%)	1207:1228	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	6	5	theme	high	1207:1210	arg1	%					1227:1227	22%	1225:1227	22%	1225:1227	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	6	6	theme	unusable/unmarketable	1331:1351	arg1	limits					1353:1358	unusable/unmarketable limits	1331:1358	unusable/unmarketable limits based on their visual quality score	1331:1394	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	6	7	theme	uncoated	1297:1304	arg1	tomatoes					1306:1313	uncoated tomatoes	1297:1313	uncoated tomatoes	1297:1313	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	4	8	dep	%	956:956	arg1	10					954:955	10	954:955	10	954:955	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	6	9	theme	quality	1382:1388	arg1	score					1390:1394	their visual quality score	1369:1394	their visual quality score	1369:1394	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	2	10	theme	rheological	307:317	arg1	properties					319:328	The rheological properties	303:328	The rheological properties of biocomposite OLP/CMC film-forming solutions	303:375	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	1	11	theme	okra	280:283	arg1	wastes					295:300	okra leafstalk wastes	280:300	okra leafstalk wastes	280:300	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	0	12	theme	Plant	98:102	arg1	Waste					104:108	Okra Plant Waste	93:108	Okra Plant Waste for Food Packaging Application	93:139	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	5	13	theme	thermal	1035:1041	arg1	stability					1043:1051	thermal stability	1035:1051	thermal stability	1035:1051	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	4	14	dep	10	954:955	arg1	to					951:952	to	951:952	to	951:952	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	8	15	theme	okra	1611:1614	arg1	residues					1622:1629	okra plant residues	1611:1629	okra plant residues for edible food packaging	1611:1655	This study successfully applied value-added polysaccharides derived from okra plant residues for edible food packaging.
36433011	1	16	theme	Polysaccharide-based	142:161	arg1	films					173:177	Polysaccharide-based composite films	142:177	Polysaccharide-based composite films	142:177	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	0	17	theme	Food	114:117	arg1	Application					129:139	Food Packaging Application	114:139	Food Packaging Application	114:139	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	7	18	theme	coated	1460:1465	arg1	tomatoes					1467:1474	coated tomatoes	1460:1474	coated tomatoes	1460:1474	In contrast, OLP/CMC effectively minimized quality losses, and coated tomatoes exceeded the limit of marketability after 14 days of storage.
36433011	3	19	theme	fluid	555:559	arg1	characteristics					561:575	pseudo-plastic fluid characteristics	540:575	pseudo-plastic fluid characteristics	540:575	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	20	dep	%	713:713	arg1	w/w					715:717	w/w	715:717	5 to 30 % w/w of CMC	705:724	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	21	dep	consistency	644:654	arg1	K					657:657	K	657:657	K	657:657	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	1	22	theme	leafstalk	285:293	arg1	wastes					295:300	okra leafstalk wastes	280:300	okra leafstalk wastes	280:300	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	6	23	dep	discoloration	1259:1271	arg1	21.26					1278:1282	21.26	1278:1282	21.26	1278:1282	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	1	24	theme	mucilage	200:207	arg1	polysaccharides					209:223	mucilage polysaccharides	200:223	mucilage polysaccharides (OLP)	200:229	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	1	24	theme	mucilage	200:207	arg1	OLP					226:228	OLP	226:228	OLP	226:228	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	5	25	theme	OLP/CMC	1056:1062	arg1	films					1064:1068	OLP/CMC films	1056:1068	OLP/CMC films	1056:1068	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	2	26	theme	OLP/CMC	346:352	arg1	solutions					367:375	biocomposite OLP/CMC film-forming solutions	333:375	biocomposite OLP/CMC film-forming solutions	333:375	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	8	27	attach	derived	1598:1604	arg2	polysaccharides					1582:1596	value-added polysaccharides	1570:1596	value-added polysaccharides derived from okra plant residues for edible food packaging	1570:1655	This study successfully applied value-added polysaccharides derived from okra plant residues for edible food packaging.
36433011	8	27	attach	derived	1598:1604	arg1	residues					1622:1629	okra plant residues	1611:1629	okra plant residues for edible food packaging	1611:1655	This study successfully applied value-added polysaccharides derived from okra plant residues for edible food packaging.
36433011	2	28	theme	packaging	488:496	arg1	applications					498:509	their potential food packaging applications	467:509	their potential food packaging applications	467:509	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	6	29	theme	Quality	1168:1174	arg1	deterioration					1176:1188	30 °C. Quality deterioration	1161:1188	30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26)	1161:1283	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	3	30	from	characteristics	561:575	arg1	behavior					608:615	rheological behavior	596:615	rheological behavior (n, 0.478-0.743)	596:632	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	2	31	theme	biocomposite	333:344	arg1	solutions					367:375	biocomposite OLP/CMC film-forming solutions	333:375	biocomposite OLP/CMC film-forming solutions	333:375	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	8	32	theme	edible	1635:1640	arg1	packaging					1647:1655	edible food packaging	1635:1655	edible food packaging	1635:1655	This study successfully applied value-added polysaccharides derived from okra plant residues for edible food packaging.
36433011	4	33	dep	OLP	958:960	arg1	%					956:956	%	956:956	%	956:956	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	2	34	theme	food	483:486	arg1	applications					498:509	their potential food packaging applications	467:509	their potential food packaging applications	467:509	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	7	35	theme	storage	1529:1535	arg1	days					1521:1524	14 days	1518:1524	14 days of storage	1518:1535	In contrast, OLP/CMC effectively minimized quality losses, and coated tomatoes exceeded the limit of marketability after 14 days of storage.
36433011	2	36	theme	potential	473:481	arg1	applications					498:509	their potential food packaging applications	467:509	their potential food packaging applications	467:509	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	0	37	theme	Packaging	119:127	arg1	Application					129:139	Food Packaging Application	114:139	Food Packaging Application	114:139	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	3	38	theme	OLP	700:702	arg1	content					689:695	content	689:695	content of OLP (5 to 30 % w/w of CMC)	689:725	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	39	dep	behavior	608:615	arg1	0.478-0.743					621:631	0.478-0.743	621:631	0.478-0.743	621:631	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	39	dep	behavior	608:615	arg1	n					618:618	n	618:618	n	618:618	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	40	theme	rheological	596:606	arg1	behavior					608:615	rheological behavior	596:615	rheological behavior (n, 0.478-0.743)	596:632	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	0	41	theme	Films	49:53	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	0	41	theme	Films	49:53	arg1	Development					0:10	Development	0:10	Development	0:10	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	5	42	theme	antioxidant	1010:1020	arg1	activity					1022:1029	antioxidant activity	1010:1029	antioxidant activity	1010:1029	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	6	43	theme	°C.	1164:1166	arg1	deterioration					1176:1188	30 °C. Quality deterioration	1161:1188	30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26)	1161:1283	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	8	44	theme	food	1642:1645	arg1	packaging					1647:1655	edible food packaging	1635:1655	edible food packaging	1635:1655	This study successfully applied value-added polysaccharides derived from okra plant residues for edible food packaging.
36433011	3	45	theme	flow	639:642	arg1	consistency					644:654	flow consistency	639:654	flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC)	639:725	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	0	46	theme	Biocomposite	36:47	arg1	Films					49:53	Biocomposite Films	36:53	Biocomposite Films	36:53	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	7	47	theme	marketability	1498:1510	arg1	limit					1489:1493	the limit	1485:1493	the limit of marketability	1485:1510	In contrast, OLP/CMC effectively minimized quality losses, and coated tomatoes exceeded the limit of marketability after 14 days of storage.
36433011	6	48	theme	firmness	1231:1238	arg1	loss					1240:1243	firmness loss	1231:1243	firmness loss (74.62%)	1231:1252	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	6	48	theme	firmness	1231:1238	arg1	%					1251:1251	74.62%	1246:1251	74.62%	1246:1251	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	4	49	theme	Surface	821:827	arg1	hydrophobicity					829:842	Surface hydrophobicity	821:842	Surface hydrophobicity (24%)	821:848	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	4	49	theme	Surface	821:827	arg1	%					847:847	24%	845:847	24%	845:847	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	1	50	theme	carboxymethyl	235:247	arg1	CMC					260:262	CMC	260:262	CMC	260:262	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	1	50	theme	carboxymethyl	235:247	arg1	cellulose					249:257	carboxymethyl cellulose	235:257	carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes	235:300	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	3	51	theme	barrier	792:798	arg1	properties					800:809	the physical, mechanical, and barrier properties	762:809	properties	800:809	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	0	52	attach	Derived	80:86	arg2	Polysaccharides					64:78	Polysaccharides	64:78	Polysaccharides Derived from Okra Plant Waste for Food Packaging Application	64:139	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	0	52	attach	Derived	80:86	arg1	Waste					104:108	Okra Plant Waste	93:108	Okra Plant Waste for Food Packaging Application	93:139	Development and Characterization of Biocomposite Films Based on Polysaccharides Derived from Okra Plant Waste for Food Packaging Application.
36433011	7	53	theme	quality	1440:1446	arg1	losses					1448:1453	quality losses	1440:1453	quality losses	1440:1453	In contrast, OLP/CMC effectively minimized quality losses, and coated tomatoes exceeded the limit of marketability after 14 days of storage.
36433011	5	54	theme	films	1064:1068	arg1	activity					1022:1029	antioxidant activity	1010:1029	antioxidant activity	1010:1029	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	5	54	theme	films	1064:1068	arg1	stability					1043:1051	thermal stability	1035:1051	thermal stability	1035:1051	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	5	55	theme	higher	975:980	arg1	content					982:988	a higher content	973:988	a higher content of OLP	973:995	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	5	56	dep	activity	1022:1029	arg1	the					1006:1008	the	1006:1008	the	1006:1008	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	4	57	theme	OLP/CMC	923:929	arg1	films					931:935	OLP/CMC films	923:935	OLP/CMC films containing up to 10% OLP	923:960	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	6	58	theme	cherry	1130:1135	arg1	tomatoes					1137:1144	cherry tomatoes	1130:1144	cherry tomatoes	1130:1144	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	4	59	theme	vapor	887:891	arg1	permeability					899:910	and oxygen (39%) and water vapor (32%) permeability	860:910	and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP	860:960	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	2	60	theme	solutions	367:375	arg1	properties					319:328	The rheological properties	303:328	The rheological properties of biocomposite OLP/CMC film-forming solutions	303:375	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	6	61	theme	visual	1375:1380	arg1	score					1390:1394	their visual quality score	1369:1394	their visual quality score	1369:1394	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	3	62	theme	films	814:818	arg1	mechanical					776:785	mechanical	776:785	mechanical	776:785	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	4	63	theme	water	881:885	arg1	vapor					887:891	water vapor	881:891	water vapor (32%)	881:897	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	4	63	theme	water	881:885	arg1	%					896:896	32%	894:896	32%	894:896	Surface hydrophobicity (24%) increased and oxygen (39%) and water vapor (32%) permeability reduced in OLP/CMC films containing up to 10% OLP.
36433011	5	64	theme	OLP	993:995	arg1	content					982:988	a higher content	973:988	a higher content of OLP	973:995	Moreover, a higher content of OLP enhanced the antioxidant activity and thermal stability of OLP/CMC films.
36433011	2	65	theme	film-forming	354:365	arg1	solutions					367:375	biocomposite OLP/CMC film-forming solutions	333:375	biocomposite OLP/CMC film-forming solutions	333:375	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	3	66	theme	pseudo-plastic	540:553	arg1	characteristics					561:575	pseudo-plastic fluid characteristics	540:575	pseudo-plastic fluid characteristics	540:575	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	67	theme	OLP/CMC	512:518	arg1	solutions					520:528	OLP/CMC solutions	512:528	OLP/CMC solutions	512:528	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	68	theme	1.731-9.154	660:670	arg1	K					657:657	K	657:657	K	657:657	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	8	69	theme	value-added	1570:1580	arg1	polysaccharides					1582:1596	value-added polysaccharides	1570:1596	value-added polysaccharides derived from okra plant residues for edible food packaging	1570:1655	This study successfully applied value-added polysaccharides derived from okra plant residues for edible food packaging.
36433011	1	70	theme	composite	163:171	arg1	films					173:177	Polysaccharide-based composite films	142:177	Polysaccharide-based composite films	142:177	Polysaccharide-based composite films were developed using mucilage polysaccharides (OLP) and carboxymethyl cellulose (CMC) extracted from okra leafstalk wastes.
36433011	2	71	theme	fabricated	427:436	arg1	films					438:442	fabricated films	427:442	fabricated films	427:442	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	3	72	dep	OLP	700:702	arg1	CMC					722:724	CMC	722:724	CMC	722:724	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	72	dep	OLP	700:702	arg1	%					713:713	5 to 30 %	705:713	5 to 30 % w/w of CMC	705:724	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	73	dep	30	710:711	arg1	to					707:708	to	707:708	to	707:708	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	74	theme	CMC	722:724	arg1	CMC					722:724	CMC	722:724	CMC	722:724	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	74	theme	CMC	722:724	arg1	%					713:713	5 to 30 %	705:713	5 to 30 % w/w of CMC	705:724	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	75	from	variations	748:757	arg1	properties					800:809	the physical, mechanical, and barrier properties	762:809	properties	800:809	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	3	75	from	variations	748:757	arg1	mechanical					776:785	mechanical	776:785	mechanical	776:785	OLP/CMC solutions exhibited pseudo-plastic fluid characteristics and differences in rheological behavior (n, 0.478-0.743), and flow consistency (K, 1.731-9.154) with increasing content of OLP (5 to 30 % w/w of CMC) were associated with variations in the physical, mechanical, and barrier properties of films.
36433011	2	76	theme	Power-law	406:414	arg1	model					416:420	the Power-law model	402:420	the Power-law model	402:420	The rheological properties of biocomposite OLP/CMC film-forming solutions were characterized using the Power-law model, and fabricated films were characterized for their potential food packaging applications.
36433011	6	77	theme	weight	1212:1217	arg1	loss					1219:1222	high weight loss	1207:1222	high weight loss (22%)	1207:1228	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
36433011	6	77	theme	weight	1212:1217	arg1	%					1227:1227	22%	1225:1227	22%	1225:1227	Subsequently, OLP/CMC was applied as a coating to preserve cherry tomatoes for 14 days at 30 °C. Quality deterioration characterized by high weight loss (22%), firmness loss (74.62%), and discoloration (∆E, 21.26) occurred in uncoated tomatoes and were within unusable/unmarketable limits based on their visual quality score.
35784754	8	0	used	used	1318:1321	arg2	Glc					1296:1298	Glc	1296:1298	Glc	1296:1298	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	8	0	used	used	1318:1321	arg2	markers					1339:1345	differential markers	1326:1345	differential markers	1326:1345	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	8	0	used	used	1318:1321	arg2	GalA					1304:1307	GalA	1304:1307	GalA	1304:1307	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	11	1	theme	processed	1695:1703	arg1	PM					1705:1706	processed PM	1695:1706	processed PM	1695:1706	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	4	2	from	PM	595:596	arg1	polysaccharides					559:573	the polysaccharides	555:573	the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs)	555:634	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	9	3	theme	mRNA	1461:1464	arg1	expression					1466:1475	mRNA expression	1461:1475	mRNA expression	1461:1475	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	9	4	from	expression	1466:1475	arg1	cells					1503:1507	RAW 264.7 cells	1493:1507	RAW 264.7 cells	1493:1507	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	5	5	contain	had	804:806	arg1	PPMPs					798:802	PPMPs	798:802	PPMPs	798:802	Results showed that RPMPs and PPMPs had significant differences in physicochemical properties.
35784754	5	5	contain	had	804:806	arg2	differences					820:830	significant differences	808:830	significant differences in physicochemical properties	808:860	Results showed that RPMPs and PPMPs had significant differences in physicochemical properties.
35784754	5	5	contain	had	804:806	arg1	RPMPs					788:792	RPMPs	788:792	RPMPs	788:792	Results showed that RPMPs and PPMPs had significant differences in physicochemical properties.
35784754	0	6	theme	multiflorum	118:128	arg1	Thunb					130:134	Polygonum multiflorum Thunb	108:134	Polygonum multiflorum Thunb	108:134	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	7	7	used	used	1141:1144	arg2	differences					1032:1042	significant differences	1020:1042	significant differences	1020:1042	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	4	8	theme	samples	744:750	arg1	properties					676:685	the physicochemical properties	656:685	the physicochemical properties	656:685	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	4	8	theme	samples	744:750	arg1	activity					708:715	immunomodulatory activity	691:715	immunomodulatory activity in vitro	691:724	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	4	9	theme	raw	591:593	arg1	RPMPs					599:603	RPMPs	599:603	RPMPs	599:603	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	4	9	theme	raw	591:593	arg1	PM					595:596	thirty-one raw PM	580:596	thirty-one raw PM (RPMPs)	580:604	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	0	10	theme	Polygonum	108:116	arg1	Thunb					130:134	Polygonum multiflorum Thunb	108:134	Polygonum multiflorum Thunb	108:134	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	4	11	theme	polysaccharide	729:742	arg1	samples					744:750	polysaccharide samples	729:750	polysaccharide samples	729:750	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	10	12	theme	lower	1567:1571	arg1	Mw					1573:1574	lower Mw	1567:1574	lower Mw	1567:1574	In addition, the immunomodulatory activity of PPMPs with lower Mw was significantly better than that of RPMPs.
35784754	1	13	dep	Polygonum	150:158	arg1	multiflorum					160:170	multiflorum	160:170	multiflorum	160:170	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	4	14	theme	thirty-one	580:589	arg1	RPMPs					599:603	RPMPs	599:603	RPMPs	599:603	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	4	14	theme	thirty-one	580:589	arg1	PM					595:596	thirty-one raw PM	580:596	thirty-one raw PM (RPMPs)	580:604	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	7	15	theme	molecular	1061:1069	arg1	Mw					1079:1080	Mw	1079:1080	Mw	1079:1080	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	15	theme	molecular	1061:1069	arg1	weight					1071:1076	molecular weight	1061:1076	molecular weight (Mw)	1061:1081	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	2	16	used	used	361:364	arg2	PM					281:282	PM	281:282	PM in raw or processed form	281:307	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	9	17	theme	cytokines	1480:1488	arg1	phagocytosis					1444:1455	phagocytosis	1444:1455	phagocytosis	1444:1455	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	9	17	theme	cytokines	1480:1488	arg1	expression					1466:1475	mRNA expression	1461:1475	mRNA expression	1461:1475	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	9	18	theme	immunomodulatory	1352:1367	arg1	assays					1378:1383	The immunomodulatory activity assays	1348:1383	The immunomodulatory activity assays	1348:1383	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	0	19	theme	Thunb	130:134	arg1	Processing					94:103	Processing	94:103	Processing of Polygonum multiflorum Thunb	94:134	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	7	20	theme	molar	1092:1096	arg1	<					1119:1119	p < 0.05	1117:1124	p < 0.05	1117:1124	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	20	theme	molar	1092:1096	arg1	ratio					1098:1102	the molar ratio	1088:1102	the molar ratio of Glc/GalA (p < 0.05)	1088:1125	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	21	contain	had	1016:1018	arg1	PPMPs					1010:1014	PPMPs	1010:1014	PPMPs	1010:1014	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	21	contain	had	1016:1018	arg1	RPMPs					1000:1004	RPMPs	1000:1004	RPMPs	1000:1004	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	21	contain	had	1016:1018	arg2	differences					1032:1042	significant differences	1020:1042	significant differences	1020:1042	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	2	22	theme	different	375:383	arg1	diseases					385:392	different diseases	375:392	different diseases	375:392	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	6	23	theme	galacturonic	937:948	arg1	acid					950:953	galacturonic acid	937:953	galacturonic acid	937:953	RPMPs and PPMPs were both composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
35784754	11	24	theme	standard	1759:1766	arg1	quality					1751:1757	the quality standard	1747:1766	the quality standard of PM	1747:1772	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	9	25	theme	RAW	1493:1495	arg1	cells					1503:1507	RAW 264.7 cells	1493:1507	RAW 264.7 cells	1493:1507	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	3	26	theme	main	419:422	arg1	Polysaccharides					395:409	Polysaccharides	395:409	Polysaccharides	395:409	Polysaccharides are the main component of PM, and it is unclear whether their physicochemical properties and activities change after processing.
35784754	3	26	theme	main	419:422	arg1	component					424:432	the main component	415:432	the main component of PM	415:438	Polysaccharides are the main component of PM, and it is unclear whether their physicochemical properties and activities change after processing.
35784754	7	27	theme	p	1117:1117	arg1	<					1119:1119	p < 0.05	1117:1124	p < 0.05	1117:1124	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	27	theme	p	1117:1117	arg1	ratio					1098:1102	the molar ratio	1088:1102	the molar ratio of Glc/GalA (p < 0.05)	1088:1125	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	10	28	theme	immunomodulatory	1527:1542	arg1	activity					1544:1551	the immunomodulatory activity	1523:1551	the immunomodulatory activity of PPMPs with lower Mw	1523:1574	In addition, the immunomodulatory activity of PPMPs with lower Mw was significantly better than that of RPMPs.
35784754	10	28	theme	immunomodulatory	1527:1542	arg1	better					1594:1599	better	1594:1599	better	1594:1599	In addition, the immunomodulatory activity of PPMPs with lower Mw was significantly better than that of RPMPs.
35784754	2	29	theme	processed	294:302	arg1	form					304:307	processed form	294:307	processed form	294:307	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	4	30	from	PM	625:626	arg1	polysaccharides					559:573	the polysaccharides	555:573	the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs)	555:634	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	1	31	theme	long	190:193	arg1	history					195:201	a long history	188:201	a long history of usage in traditional Chinese medicine	188:242	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	0	32	theme	Properties	27:36	arg1	Changes					0:6	Changes	0:6	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.	0:135	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	8	33	theme	composition	1217:1227	arg1	fingerprint					1187:1197	The fingerprint	1183:1197	The fingerprint of monosaccharide composition	1183:1227	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	8	34	theme	monosaccharide	1202:1215	arg1	composition					1217:1227	monosaccharide composition	1202:1227	monosaccharide composition	1202:1227	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	9	35	from	phagocytosis	1444:1455	arg1	cells					1503:1507	RAW 264.7 cells	1493:1507	RAW 264.7 cells	1493:1507	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	11	36	from	PM	1705:1706	arg1	polysaccharides					1666:1680	the polysaccharides	1662:1680	the polysaccharides from raw and processed PM	1662:1706	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	11	36	from	PM	1705:1706	arg1	understanding					1645:1657	the understanding	1641:1657	the understanding of the polysaccharides from raw and processed PM	1641:1706	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	5	37	theme	significant	808:818	arg1	differences					820:830	significant differences	808:830	significant differences in physicochemical properties	808:860	Results showed that RPMPs and PPMPs had significant differences in physicochemical properties.
35784754	0	38	theme	Physicochemical	11:25	arg1	Properties					27:36	Physicochemical Properties	11:36	Physicochemical Properties	11:36	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	1	39	used	utilized	265:272	arg2	roots					141:145	The roots	137:145	The roots of Polygonum multiflorum Thunb (PM)	137:181	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	11	40	from	understanding	1645:1657	arg1	raw					1687:1689	raw	1687:1689	raw	1687:1689	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	11	40	from	understanding	1645:1657	arg1	PM					1705:1706	processed PM	1695:1706	processed PM	1695:1706	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	0	41	theme	Immunomodulatory	42:57	arg1	Activity					59:66	Immunomodulatory Activity	42:66	Immunomodulatory Activity	42:66	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	3	42	theme	physicochemical	473:487	arg1	properties					489:498	their physicochemical properties	467:498	their physicochemical properties	467:498	Polysaccharides are the main component of PM, and it is unclear whether their physicochemical properties and activities change after processing.
35784754	7	43	theme	significant	1020:1030	arg1	differences					1032:1042	significant differences	1020:1042	significant differences	1020:1042	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	9	44	theme	activity	1369:1376	arg1	assays					1378:1383	The immunomodulatory activity assays	1348:1383	The immunomodulatory activity assays	1348:1383	The immunomodulatory activity assays indicated that RPMPs and PPMPs could significantly enhance phagocytosis and mRNA expression of cytokines in RAW 264.7 cells.
35784754	1	45	theme	usage	206:210	arg1	history					195:201	a long history	188:201	a long history of usage in traditional Chinese medicine	188:242	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	7	46	from	differences	1032:1042	arg1	Mw					1079:1080	Mw	1079:1080	Mw	1079:1080	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	46	from	differences	1032:1042	arg1	yields					1053:1058	their yields	1047:1058	their yields	1047:1058	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	46	from	differences	1032:1042	arg1	ratio					1098:1102	the molar ratio	1088:1102	the molar ratio of Glc/GalA (p < 0.05)	1088:1125	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	46	from	differences	1032:1042	arg1	<					1119:1119	p < 0.05	1117:1124	p < 0.05	1117:1124	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	46	from	differences	1032:1042	arg1	weight					1071:1076	molecular weight	1061:1076	molecular weight (Mw)	1061:1081	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	1	47	contain	have	183:186	arg1	roots					141:145	The roots	137:145	The roots of Polygonum multiflorum Thunb (PM)	137:181	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	1	47	contain	have	183:186	arg2	history					195:201	a long history	188:201	a long history of usage in traditional Chinese medicine	188:242	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	11	48	theme	polysaccharides	1666:1680	arg1	understanding					1645:1657	the understanding	1641:1657	the understanding of the polysaccharides from raw and processed PM	1641:1706	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	4	49	theme	processed	615:623	arg1	PPMPs					629:633	PPMPs	629:633	PPMPs	629:633	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	4	49	theme	processed	615:623	arg1	PM					625:626	PM	625:626	nine processed PM (PPMPs)	610:634	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	11	50	theme	PM	1771:1772	arg1	quality					1751:1757	the quality standard	1747:1766	the quality standard of PM	1747:1772	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	0	51	theme	Activity	59:66	arg1	Changes					0:6	Changes	0:6	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.	0:135	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	6	52	theme	glucuronic	920:929	arg1	acid					931:934	glucuronic acid	920:934	glucuronic acid	920:934	RPMPs and PPMPs were both composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, and arabinose.
35784754	11	53	from	raw	1687:1689	arg1	polysaccharides					1666:1680	the polysaccharides	1662:1680	the polysaccharides from raw and processed PM	1662:1706	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	11	53	from	raw	1687:1689	arg1	understanding					1645:1657	the understanding	1641:1657	the understanding of the polysaccharides from raw and processed PM	1641:1706	This study furthers the understanding of the polysaccharides from raw and processed PM and provides a reference for improving the quality standard of PM.
35784754	1	54	from	history	195:201	arg1	medicine					235:242	traditional Chinese medicine	215:242	traditional Chinese medicine	215:242	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	2	55	theme	biological	323:332	arg1	activities					334:343	different biological activities	313:343	different biological activities	313:343	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	8	56	theme	differential	1326:1337	arg1	Glc					1296:1298	Glc	1296:1298	Glc	1296:1298	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	8	56	theme	differential	1326:1337	arg1	GalA					1304:1307	GalA	1304:1307	GalA	1304:1307	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	8	56	theme	differential	1326:1337	arg1	markers					1339:1345	differential markers	1326:1345	differential markers	1326:1345	The fingerprint of monosaccharide composition was analyzed by chemometrics, and it was further demonstrated that Glc and GalA could be used as differential markers.
35784754	10	57	theme	PPMPs	1556:1560	arg1	activity					1544:1551	the immunomodulatory activity	1523:1551	the immunomodulatory activity of PPMPs with lower Mw	1523:1574	In addition, the immunomodulatory activity of PPMPs with lower Mw was significantly better than that of RPMPs.
35784754	10	57	theme	PPMPs	1556:1560	arg1	better					1594:1599	better	1594:1599	better	1594:1599	In addition, the immunomodulatory activity of PPMPs with lower Mw was significantly better than that of RPMPs.
35784754	5	58	from	differences	820:830	arg1	properties					851:860	physicochemical properties	835:860	physicochemical properties	835:860	Results showed that RPMPs and PPMPs had significant differences in physicochemical properties.
35784754	2	59	theme	different	313:321	arg1	activities					334:343	different biological activities	313:343	different biological activities	313:343	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	1	60	theme	traditional	215:225	arg1	medicine					235:242	traditional Chinese medicine	215:242	traditional Chinese medicine	215:242	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	4	61	theme	immunomodulatory	691:706	arg1	activity					708:715	immunomodulatory activity	691:715	immunomodulatory activity in vitro	691:724	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35784754	10	62	with	activity	1544:1551	arg1	Mw					1573:1574	lower Mw	1567:1574	lower Mw	1567:1574	In addition, the immunomodulatory activity of PPMPs with lower Mw was significantly better than that of RPMPs.
35784754	1	63	theme	Polygonum	150:158	arg1	Thunb					172:176	Polygonum multiflorum Thunb	150:176	Polygonum multiflorum Thunb (PM)	150:181	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	1	63	theme	Polygonum	150:158	arg1	PM					179:180	PM	179:180	PM	179:180	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	1	64	theme	Chinese	227:233	arg1	medicine					235:242	traditional Chinese medicine	215:242	traditional Chinese medicine	215:242	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	3	65	theme	PM	437:438	arg1	Polysaccharides					395:409	Polysaccharides	395:409	Polysaccharides	395:409	Polysaccharides are the main component of PM, and it is unclear whether their physicochemical properties and activities change after processing.
35784754	3	65	theme	PM	437:438	arg1	component					424:432	the main component	415:432	the main component of PM	415:438	Polysaccharides are the main component of PM, and it is unclear whether their physicochemical properties and activities change after processing.
35784754	5	66	theme	physicochemical	835:849	arg1	properties					851:860	physicochemical properties	835:860	physicochemical properties	835:860	Results showed that RPMPs and PPMPs had significant differences in physicochemical properties.
35784754	2	67	from	PM	281:282	arg1	raw					287:289	raw	287:289	raw	287:289	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	2	67	from	PM	281:282	arg1	form					304:307	processed form	294:307	processed form	294:307	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	0	68	theme	Polysaccharides	71:85	arg1	Properties					27:36	Physicochemical Properties	11:36	Physicochemical Properties	11:36	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	0	68	theme	Polysaccharides	71:85	arg1	Activity					59:66	Immunomodulatory Activity	42:66	Immunomodulatory Activity	42:66	Changes of Physicochemical Properties and Immunomodulatory Activity of Polysaccharides During Processing of Polygonum multiflorum Thunb.
35784754	2	69	contain	has	309:311	arg1	PM					281:282	PM	281:282	PM in raw or processed form	281:307	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	2	69	contain	has	309:311	arg2	activities					334:343	different biological activities	313:343	different biological activities	313:343	PM in raw or processed form has different biological activities and is commonly used to treat different diseases.
35784754	7	70	theme	Glc/GalA	1107:1114	arg1	ratio					1098:1102	the molar ratio	1088:1102	the molar ratio of Glc/GalA (p < 0.05)	1088:1125	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	70	theme	Glc/GalA	1107:1114	arg1	Mw					1079:1080	Mw	1079:1080	Mw	1079:1080	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	70	theme	Glc/GalA	1107:1114	arg1	yields					1053:1058	their yields	1047:1058	their yields	1047:1058	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	70	theme	Glc/GalA	1107:1114	arg1	weight					1071:1076	molecular weight	1061:1076	molecular weight (Mw)	1061:1081	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	70	theme	Glc/GalA	1107:1114	arg1	<					1119:1119	p < 0.05	1117:1124	p < 0.05	1117:1124	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	7	71	theme	processed	1169:1177	arg1	PM					1179:1180	processed PM	1169:1180	processed PM	1169:1180	However, RPMPs and PPMPs had significant differences in their yields, molecular weight (Mw), and the molar ratio of Glc/GalA (p < 0.05), which can be used to distinguish raw and processed PM.
35784754	1	72	theme	Thunb	172:176	arg1	roots					141:145	The roots	137:145	The roots of Polygonum multiflorum Thunb (PM)	137:181	The roots of Polygonum multiflorum Thunb (PM) have a long history of usage in traditional Chinese medicine and are still widely utilized today.
35784754	4	73	theme	physicochemical	660:674	arg1	properties					676:685	the physicochemical properties	656:685	the physicochemical properties	656:685	In this study, the polysaccharides from thirty-one raw PM (RPMPs) and nine processed PM (PPMPs) were extracted, and the physicochemical properties and immunomodulatory activity in vitro of polysaccharide samples were evaluated.
35696081	8	0	theme	N-	1145:1146	arg1	residues					1128:1135	the neighboring residues	1112:1135	the neighboring residues of each N- and O-linked glycosylation site	1112:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	0	theme	N-	1145:1146	arg1	N-					1145:1146	N-	1145:1146	N-	1145:1146	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	0	theme	N-	1145:1146	arg1	glycosylation					1161:1173	O-linked glycosylation	1152:1173	O-linked glycosylation	1152:1173	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	1	dep	sequence-based	1039:1052	arg1	predicted					1055:1063	predicted	1055:1063	predicted	1055:1063	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	5	2	theme	large	625:629	arg1	dataset					631:637	a large dataset	623:637	a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources	623:746	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	4	3	contain	containing	484:493	arg2	dataset					507:513	the largest dataset	495:513	the largest dataset	495:513	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	4	3	contain	containing	484:493	arg1	framework					474:482	the SPRINT-Gly framework	459:482	the SPRINT-Gly framework containing the largest dataset	459:513	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	4	4	theme	given	567:571	arg1	sequence					581:588	a given protein sequence	565:588	a given protein sequence	565:588	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	8	5	theme	structural	1065:1074	arg1	information					1097:1107	sequence-based, predicted structural, and physicochemical information	1039:1107	sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site	1039:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	6	6	theme	putative	800:807	arg1	N-					809:810	putative N-	800:810	putative N-	800:810	We then introduce the SPRINT-Gly method to predict putative N- and O-linked sites.
35696081	1	7	theme	modifications	161:173	arg1	PTM					176:178	PTM	176:178	PTM	176:178	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	1	7	theme	modifications	161:173	arg1	one					119:121	one	119:121	one	119:121	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	1	7	theme	modifications	161:173	arg1	modifications					161:173	the most complex posttranslational modifications	126:173	the most complex posttranslational modifications (PTM) that play a fundamental role in protein function	126:228	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	7	8	theme	machine	848:854	arg1	approach					871:878	a machine learning-based approach	846:878	a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately	846:999	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	7	8	theme	machine	848:854	arg1	SPRINT-Gly					832:841	SPRINT-Gly	832:841	SPRINT-Gly	832:841	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	10	9	from	https	1521:1525	arg1	predictor					1508:1516	an online and stand-alone predictor	1482:1516	an online and stand-alone predictor at https://sparks-lab.org/server/sprint-gly/	1482:1561	SPRINT-Gly is publicly available as an online and stand-alone predictor at https://sparks-lab.org/server/sprint-gly/ .
35696081	5	10	theme	proteins	705:712	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	10	theme	proteins	705:712	arg1	sites					680:684	N- and O-linked glycosylation sites	650:684	N- and O-linked glycosylation sites	650:684	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	4	11	theme	largest	499:505	arg1	dataset					507:513	the largest dataset	495:513	the largest dataset	495:513	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	2	12	theme	experimental	282:293	arg1	approaches					295:304	experimental approaches	282:304	experimental approaches	282:304	Identification and annotation of these sites using experimental approaches are challenging and time consuming.
35696081	7	13	theme	learning-based	856:869	arg1	approach					871:878	a machine learning-based approach	846:878	a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately	846:999	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	7	13	theme	learning-based	856:869	arg1	SPRINT-Gly					832:841	SPRINT-Gly	832:841	SPRINT-Gly	832:841	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	8	14	dep	built	1016:1020	arg1	training					1187:1194	training	1187:1194	training deep learning neural network and support vector machine as classifiers	1187:1265	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	14	dep	built	1016:1020	arg1	incorporating					1025:1037	incorporating	1025:1037	incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site	1025:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	5	15	theme	N-	650:651	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	15	theme	N-	650:651	arg1	sites					680:684	N- and O-linked glycosylation sites	650:684	N- and O-linked glycosylation sites	650:684	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	7	16	theme	glycosylation	936:948	arg1	sites					950:954	glycosylation sites	936:954	glycosylation sites in both human and mouse proteins	936:987	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	1	17	theme	posttranslational	143:159	arg1	PTM					176:178	PTM	176:178	PTM	176:178	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	1	17	theme	posttranslational	143:159	arg1	modifications					161:173	the most complex posttranslational modifications	126:173	the most complex posttranslational modifications (PTM) that play a fundamental role in protein function	126:228	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	4	18	theme	protein	573:579	arg1	sequence					581:588	a given protein sequence	565:588	a given protein sequence	565:588	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	8	19	theme	sequence-based	1039:1052	arg1	information					1097:1107	sequence-based, predicted structural, and physicochemical information	1039:1107	sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site	1039:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	2	20	theme	sites	270:274	arg1	annotation					250:259	annotation	250:259	annotation	250:259	Identification and annotation of these sites using experimental approaches are challenging and time consuming.
35696081	2	20	theme	sites	270:274	arg1	Identification					231:244	Identification	231:244	Identification	231:244	Identification and annotation of these sites using experimental approaches are challenging and time consuming.
35696081	8	21	theme	vector	1237:1242	arg1	machine					1244:1250	support vector machine	1229:1250	support vector machine	1229:1250	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	21	theme	vector	1237:1242	arg1	network					1217:1223	deep learning neural network	1196:1223	deep learning neural network	1196:1223	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	21	theme	vector	1237:1242	arg1	classifiers					1255:1265	classifiers	1255:1265	classifiers	1255:1265	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	0	22	theme	Glycosylation	44:56	arg1	Prediction					14:23	Computational Prediction	0:23	Computational Prediction of N- and O-Linked Glycosylation	0:56	Computational Prediction of N- and O-Linked Glycosylation Sites for Human and Mouse Proteins.
35696081	0	23	theme	O-Linked	35:42	arg1	Glycosylation					44:56	O-Linked Glycosylation	35:56	O-Linked Glycosylation	35:56	Computational Prediction of N- and O-Linked Glycosylation Sites for Human and Mouse Proteins.
35696081	6	24	theme	SPRINT-Gly	771:780	arg1	method					782:787	the SPRINT-Gly method	767:787	the SPRINT-Gly method to predict putative N- and O-linked sites	767:829	We then introduce the SPRINT-Gly method to predict putative N- and O-linked sites.
35696081	0	25	theme	Computational	0:12	arg1	Prediction					14:23	Computational Prediction	0:23	Computational Prediction of N- and O-Linked Glycosylation	0:56	Computational Prediction of N- and O-Linked Glycosylation Sites for Human and Mouse Proteins.
35696081	8	26	theme	support	1229:1235	arg1	machine					1244:1250	support vector machine	1229:1250	support vector machine	1229:1250	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	26	theme	support	1229:1235	arg1	network					1217:1223	deep learning neural network	1196:1223	deep learning neural network	1196:1223	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	26	theme	support	1229:1235	arg1	classifiers					1255:1265	classifiers	1255:1265	classifiers	1255:1265	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	4	27	theme	SPRINT-Gly	463:472	arg1	framework					474:482	the SPRINT-Gly framework	459:482	the SPRINT-Gly framework containing the largest dataset	459:513	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	6	28	link	O-linked	816:823	arg1	sites					825:829	O-linked sites	816:829	O-linked sites	816:829	We then introduce the SPRINT-Gly method to predict putative N- and O-linked sites.
35696081	3	29	theme	fast	376:379	arg1	methods					409:415	fast and efficient computational methods	376:415	fast and efficient computational methods	376:415	Hence, there is a demand to build fast and efficient computational methods to address this problem.
35696081	4	30	theme	glycosylation	541:553	arg1	sites					555:559	glycosylation sites	541:559	glycosylation sites for a given protein sequence	541:588	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	7	31	theme	trained	906:912	arg1	models					925:930	trained predictive models	906:930	trained predictive models for glycosylation sites in both human and mouse proteins	906:987	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	8	32	dep	N-	1145:1146	arg1	each					1140:1143	each	1140:1143	each	1140:1143	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	32	dep	N-	1145:1146	arg1	site					1175:1178	site	1175:1178	site	1175:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	7	33	dep	human	964:968	arg1	proteins					980:987	proteins	980:987	proteins	980:987	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	0	34	theme	N-	28:29	arg1	Prediction					14:23	Computational Prediction	0:23	Computational Prediction of N- and O-Linked Glycosylation	0:56	Computational Prediction of N- and O-Linked Glycosylation Sites for Human and Mouse Proteins.
35696081	8	35	theme	glycosylation	1161:1173	arg1	residues					1128:1135	the neighboring residues	1112:1135	the neighboring residues of each N- and O-linked glycosylation site	1112:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	35	theme	glycosylation	1161:1173	arg1	N-					1145:1146	N-	1145:1146	N-	1145:1146	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	35	theme	glycosylation	1161:1173	arg1	glycosylation					1161:1173	O-linked glycosylation	1152:1173	O-linked glycosylation	1152:1173	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	7	36	theme	predictive	914:923	arg1	models					925:930	trained predictive models	906:930	trained predictive models for glycosylation sites in both human and mouse proteins	906:987	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	0	37	dep	Human	68:72	arg1	Proteins					84:91	Proteins	84:91	Proteins	84:91	Computational Prediction of N- and O-Linked Glycosylation Sites for Human and Mouse Proteins.
35696081	10	38	theme	online	1485:1490	arg1	predictor					1508:1516	an online and stand-alone predictor	1482:1516	an online and stand-alone predictor at https://sparks-lab.org/server/sprint-gly/	1482:1561	SPRINT-Gly is publicly available as an online and stand-alone predictor at https://sparks-lab.org/server/sprint-gly/ .
35696081	9	39	theme	higher	1340:1345	arg1	coefficient					1369:1379	higher Matthew's correlation coefficient	1340:1379	50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction	1336:1429	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	5	40	theme	human	689:693	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	41	gly	glycosylation	666:678	arg2	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	41	gly	glycosylation	666:678	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	41	gly	glycosylation	666:678	arg2	sites					680:684	N- and O-linked glycosylation sites	650:684	N- and O-linked glycosylation sites	650:684	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	9	42	dep	%	1338:1338	arg1	coefficient					1369:1379	higher Matthew's correlation coefficient	1340:1379	50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction	1336:1429	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	3	43	theme	computational	395:407	arg1	methods					409:415	fast and efficient computational methods	376:415	fast and efficient computational methods	376:415	Hence, there is a demand to build fast and efficient computational methods to address this problem.
35696081	4	44	theme	sites	555:559	arg1	model					532:536	a prediction model	519:536	a prediction model of glycosylation sites for a given protein sequence	519:588	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	4	44	theme	sites	555:559	arg1	framework					474:482	the SPRINT-Gly framework	459:482	the SPRINT-Gly framework containing the largest dataset	459:513	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	8	45	theme	physicochemical	1081:1095	arg1	information					1097:1107	sequence-based, predicted structural, and physicochemical information	1039:1107	sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site	1039:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	10	46	theme	stand-alone	1496:1506	arg1	predictor					1508:1516	an online and stand-alone predictor	1482:1516	an online and stand-alone predictor at https://sparks-lab.org/server/sprint-gly/	1482:1561	SPRINT-Gly is publicly available as an online and stand-alone predictor at https://sparks-lab.org/server/sprint-gly/ .
35696081	1	47	theme	fundamental	193:203	arg1	role					205:208	a fundamental role	191:208	a fundamental role	191:208	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	8	48	theme	learning	1201:1208	arg1	machine					1244:1250	support vector machine	1229:1250	support vector machine	1229:1250	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	48	theme	learning	1201:1208	arg1	network					1217:1223	deep learning neural network	1196:1223	deep learning neural network	1196:1223	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	48	theme	learning	1201:1208	arg1	classifiers					1255:1265	classifiers	1255:1265	classifiers	1255:1265	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	5	49	theme	mouse	699:703	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	9	50	dep	N-	1385:1386	arg1	prediction					1420:1429	site prediction	1415:1429	site prediction	1415:1429	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	8	51	theme	deep	1196:1199	arg1	learning					1201:1208	deep learning	1196:1208	deep learning neural network	1196:1223	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	9	52	link	O-linked	1392:1399	arg1	glycosylation					1401:1413	O-linked glycosylation	1392:1413	O-linked glycosylation	1392:1413	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	7	53	from	sites	950:954	arg1	mouse					974:978	mouse	974:978	mouse	974:978	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	7	53	from	sites	950:954	arg1	human					964:968	human	964:968	human	964:968	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	8	54	theme	O-linked	1152:1159	arg1	glycosylation					1161:1173	O-linked glycosylation	1152:1173	O-linked glycosylation	1152:1173	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	55	theme	residues	1128:1135	arg1	information					1097:1107	sequence-based, predicted structural, and physicochemical information	1039:1107	sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site	1039:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	4	56	gly	glycosylation	541:553	arg2	sites					555:559	glycosylation sites	541:559	glycosylation sites for a given protein sequence	541:588	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	3	57	theme	efficient	385:393	arg1	methods					409:415	fast and efficient computational methods	376:415	fast and efficient computational methods	376:415	Hence, there is a demand to build fast and efficient computational methods to address this problem.
35696081	5	58	theme	O-linked	657:664	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	58	theme	O-linked	657:664	arg1	sites					680:684	N- and O-linked glycosylation sites	650:684	N- and O-linked glycosylation sites	650:684	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	7	59	theme	models	925:930	arg1	number					896:901	a number	894:901	a number of trained predictive models for glycosylation sites in both human and mouse proteins	894:987	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	9	60	theme	existing	1298:1305	arg1	methods					1307:1313	other existing methods	1292:1313	other existing methods	1292:1313	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	8	61	theme	neural	1210:1215	arg1	machine					1244:1250	support vector machine	1229:1250	support vector machine	1229:1250	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	61	theme	neural	1210:1215	arg1	network					1217:1223	deep learning neural network	1196:1223	deep learning neural network	1196:1223	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	61	theme	neural	1210:1215	arg1	classifiers					1255:1265	classifiers	1255:1265	classifiers	1255:1265	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	4	62	theme	prediction	521:530	arg1	model					532:536	a prediction model	519:536	a prediction model of glycosylation sites for a given protein sequence	519:588	Here, we present the SPRINT-Gly framework containing the largest dataset and a prediction model of glycosylation sites for a given protein sequence.
35696081	5	63	theme	glycosylation	666:678	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	63	theme	glycosylation	666:678	arg1	sites					680:684	N- and O-linked glycosylation sites	650:684	N- and O-linked glycosylation sites	650:684	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	6	64	theme	O-linked	816:823	arg1	sites					825:829	O-linked sites	816:829	O-linked sites	816:829	We then introduce the SPRINT-Gly method to predict putative N- and O-linked sites.
35696081	1	65	theme	Protein	94:100	arg1	glycosylation					102:114	Protein glycosylation	94:114	Protein glycosylation	94:114	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	5	66	contain	containing	639:648	arg1	dataset					631:637	a large dataset	623:637	a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources	623:746	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	66	contain	containing	639:648	arg2	sites					680:684	N- and O-linked glycosylation sites	650:684	N- and O-linked glycosylation sites	650:684	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	66	contain	containing	639:648	arg2	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	8	67	theme	neighboring	1116:1126	arg1	residues					1128:1135	the neighboring residues	1112:1135	the neighboring residues of each N- and O-linked glycosylation site	1112:1178	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	67	theme	neighboring	1116:1126	arg1	N-					1145:1146	N-	1145:1146	N-	1145:1146	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	8	67	theme	neighboring	1116:1126	arg1	glycosylation					1161:1173	O-linked glycosylation	1152:1173	O-linked glycosylation	1152:1173	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	5	68	link	O-linked	657:664	arg1	proteins					705:712	human and mouse proteins	689:712	human and mouse proteins	689:712	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	5	68	link	O-linked	657:664	arg1	sites					680:684	N- and O-linked glycosylation sites	650:684	N- and O-linked glycosylation sites	650:684	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	1	69	theme	protein	213:219	arg1	function					221:228	protein function	213:228	protein function	213:228	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	5	70	theme	different	730:738	arg1	sources					740:746	different sources	730:746	different sources	730:746	In this framework, we construct a large dataset containing N- and O-linked glycosylation sites of human and mouse proteins, collected from different sources.
35696081	9	71	theme	site	1415:1418	arg1	prediction					1420:1429	site prediction	1415:1429	site prediction	1415:1429	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	1	72	theme	most	130:133	arg1	PTM					176:178	PTM	176:178	PTM	176:178	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	1	72	theme	most	130:133	arg1	modifications					161:173	the most complex posttranslational modifications	126:173	the most complex posttranslational modifications (PTM) that play a fundamental role in protein function	126:228	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	9	73	theme	O-linked	1392:1399	arg1	glycosylation					1401:1413	O-linked glycosylation	1392:1413	O-linked glycosylation	1392:1413	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	7	74	gly	glycosylation	936:948	arg2	sites					950:954	glycosylation sites	936:954	glycosylation sites in both human and mouse proteins	936:987	SPRINT-Gly is a machine learning-based approach consisting of a number of trained predictive models for glycosylation sites in both human and mouse proteins, separately.
35696081	9	75	theme	other	1292:1296	arg1	methods					1307:1313	other existing methods	1292:1313	other existing methods	1292:1313	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
35696081	8	76	link	O-linked	1152:1159	arg1	glycosylation					1161:1173	O-linked glycosylation	1152:1173	O-linked glycosylation	1152:1173	The method is built by incorporating sequence-based, predicted structural, and physicochemical information of the neighboring residues of each N- and O-linked glycosylation site and by training deep learning neural network and support vector machine as classifiers.
35696081	1	77	theme	complex	135:141	arg1	PTM					176:178	PTM	176:178	PTM	176:178	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	1	77	theme	complex	135:141	arg1	modifications					161:173	the most complex posttranslational modifications	126:173	the most complex posttranslational modifications (PTM) that play a fundamental role in protein function	126:228	Protein glycosylation is one of the most complex posttranslational modifications (PTM) that play a fundamental role in protein function.
35696081	9	78	theme	correlation	1357:1367	arg1	coefficient					1369:1379	higher Matthew's correlation coefficient	1340:1379	50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction	1336:1429	SPRINT-Gly outperformed other existing methods by achieving 18% and 50% higher Matthew's correlation coefficient for N- and O-linked glycosylation site prediction, respectively.
36052065	14	0	theme	gut	2531:2533	arg1	dysbiosis					2535:2543	obesity-associated gut dysbiosis and metabolic inflammation	2512:2570	dysbiosis	2535:2543	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	9	1	theme	obese	1708:1712	arg1	mice					1714:1717	obese mice	1708:1717	obese mice	1708:1717	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	14	2	theme	key	2416:2418	arg1	markers					2428:2434	key mucosal markers	2416:2434	key mucosal markers involved in the repair of epithelial barrier integrity	2416:2489	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	10	3	from	expression	1923:1932	arg1	mice					1968:1971	obese mice	1962:1971	obese mice	1962:1971	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	7	4	theme	barrier	1524:1530	arg1	function					1532:1539	improved intestinal barrier function	1504:1539	improved intestinal barrier function	1504:1539	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	2	5	theme	gut	569:571	arg1	composition					584:594	gut microbiota composition	569:594	gut microbiota composition	569:594	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	7	6	theme	improved	1504:1511	arg1	function					1532:1539	improved intestinal barrier function	1504:1539	improved intestinal barrier function	1504:1539	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	12	7	theme	intestinal	2158:2167	arg1	system					2176:2181	The intestinal immune system	2154:2181	The intestinal immune system	2154:2181	The intestinal immune system was also shaped by dietary factor recognition.
36052065	10	8	theme	obese	1962:1966	arg1	mice					1968:1971	obese mice	1962:1971	obese mice	1962:1971	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	14	9	theme	microbiota	2380:2389	arg1	composition					2391:2401	gut microbiota composition	2376:2401	gut microbiota composition	2376:2401	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	14	10	theme	barrier	2473:2479	arg1	integrity					2481:2489	epithelial barrier integrity	2462:2489	epithelial barrier integrity	2462:2489	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	9	11	theme	pro-inflammatory	1745:1760	arg1	response					1769:1776	the pro-inflammatory immune response	1741:1776	the pro-inflammatory immune response	1741:1776	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	2	12	link	agave-derived	512:524	arg1	neo-fructans					526:537	a highly branched agave-derived neo-fructans	494:537	a highly branched agave-derived neo-fructans	494:537	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	14	13	theme	obesity-associated	2512:2529	arg1	dysbiosis					2535:2543	obesity-associated gut dysbiosis and metabolic inflammation	2512:2570	dysbiosis	2535:2543	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	6	14	theme	increased	1262:1270	arg1	level					1272:1276	a significantly increased level	1246:1276	a significantly increased level of butyrate in obese mice receiving AG	1246:1315	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	2	15	theme	neo-fructans	526:537	arg1	effect					418:423	the effect	414:423	the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	414:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	7	16	theme	plasma	1424:1429	arg1	LBP					1431:1433	plasma LBP	1424:1433	plasma LBP	1424:1433	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	5	17	dep	either	989:994	arg1	alone					996:1000	alone	996:1000	alone	996:1000	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	13	18	theme	receptors	2322:2330	arg1	expression					2291:2300	the expression	2287:2300	the expression of aryl hydrocarbon receptors (Ahr)	2287:2336	Indeed, the combination of CP+AG significantly increased the expression of aryl hydrocarbon receptors (Ahr).
36052065	9	19	theme	different	1786:1794	arg1	pathways					1806:1813	different signaling pathways	1786:1813	different signaling pathways	1786:1813	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	5	20	dep	AG	985:986	arg1	either					989:994	either	989:994	either	989:994	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	5	20	dep	AG	985:986	arg1	combined					1005:1012	combined	1005:1012	combined	1005:1012	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	10	21	theme	TLR	1876:1878	arg1	expression					1890:1899	toll-like receptor (TLR)-2 (Tlr2) expression	1856:1899	toll-like receptor (TLR)-2 (Tlr2) expression	1856:1899	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	2	22	theme	high-fat	687:694	arg1	diet					720:723	an obesogenic high-fat and high-sucrose (HFHS) diet	673:723	an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	673:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	11	23	contain	containing	2119:2128	arg1	domain					2112:2117	NOD-like receptor family pyrin domain	2081:2117	NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome	2081:2151	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	11	23	contain	containing	2119:2128	arg2	inflammasome					2140:2151	6 (Nlrp6) inflammasome	2130:2151	6 (Nlrp6) inflammasome	2130:2151	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	10	24	theme	receptor	1866:1873	arg1	TLR					1876:1878	toll-like receptor (TLR)-2	1856:1881	toll-like receptor (TLR)-2 (Tlr2) expression	1856:1899	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	10	24	theme	receptor	1866:1873	arg1	Tlr2					1884:1887	Tlr2	1884:1887	Tlr2	1884:1887	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	6	25	theme	obese	1293:1297	arg1	mice					1299:1302	obese mice	1293:1302	obese mice receiving AG	1293:1315	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	11	26	theme	domain	2112:2117	arg1	expression					2067:2076	the expression	2063:2076	the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome	2063:2151	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	4	27	with	supplementation	807:821	arg1	CP					828:829	CP	828:829	CP	828:829	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	11	28	theme	marker	2030:2035	arg1	Foxp3					2037:2041	the anti-inflammatory marker Foxp3	2008:2041	the anti-inflammatory marker Foxp3	2008:2041	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	6	29	with	consistent	1230:1239	arg1	level					1272:1276	a significantly increased level	1246:1276	a significantly increased level of butyrate in obese mice receiving AG	1246:1315	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	4	30	theme	metabolic	967:975	arg1	health					977:982	metabolic health	967:982	metabolic health	967:982	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	7	31	theme	CP+AG-supplemented	1357:1374	arg1	mice					1385:1388	CP+AG-supplemented HFHS-fed mice	1357:1388	CP+AG-supplemented HFHS-fed mice	1357:1388	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	5	32	theme	Faecalibaculum	1104:1117	arg1	rodentium					1119:1127	Faecalibaculum rodentium	1104:1127	Faecalibaculum rodentium	1104:1127	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	8	33	theme	Gut	1542:1544	arg1	microbiota					1546:1555	Gut microbiota	1542:1555	Gut microbiota	1542:1555	Gut microbiota and derived metabolites interact with the immunological factors to improve intestinal epithelium barrier function.
36052065	2	34	theme	high-sucrose	700:711	arg1	diet					720:723	an obesogenic high-fat and high-sucrose (HFHS) diet	673:723	an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	673:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	0	35	theme	microbiota	71:80	arg1	composition					82:92	microbiota composition	71:92	microbiota composition	71:92	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	13	36	theme	aryl	2305:2308	arg1	receptors					2322:2330	aryl hydrocarbon receptors	2305:2330	aryl hydrocarbon receptors (Ahr)	2305:2336	Indeed, the combination of CP+AG significantly increased the expression of aryl hydrocarbon receptors (Ahr).
36052065	13	36	theme	aryl	2305:2308	arg1	Ahr					2333:2335	Ahr	2333:2335	Ahr	2333:2335	Indeed, the combination of CP+AG significantly increased the expression of aryl hydrocarbon receptors (Ahr).
36052065	4	37	theme	beneficial	931:940	arg1	bacteria					942:949	a beneficial bacteria	929:949	a beneficial bacteria associated with metabolic health	929:982	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	4	37	theme	beneficial	931:940	arg1	muciniphila					916:926	Akkermansia muciniphila	904:926	Akkermansia muciniphila	904:926	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	0	38	theme	gut	110:112	arg1	function					122:129	gut barrier function	110:129	gut barrier function	110:129	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	11	39	theme	NOD-like	2081:2088	arg1	domain					2112:2117	NOD-like receptor family pyrin domain	2081:2117	NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome	2081:2151	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	2	40	theme	mice	664:667	arg1	immunomodulation					632:647	mucosal immunomodulation	624:647	mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	624:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	40	theme	mice	664:667	arg1	composition					584:594	gut microbiota composition	569:594	gut microbiota composition	569:594	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	40	theme	mice	664:667	arg1	endotoxemia					607:617	metabolic endotoxemia	597:617	metabolic endotoxemia	597:617	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	40	theme	mice	664:667	arg1	response					559:566	cardiometabolic response	543:566	cardiometabolic response	543:566	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	0	41	theme	Cranberry	0:8	arg1	polyphenols					10:20	Cranberry polyphenols	0:20	Cranberry polyphenols	0:20	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	8	42	theme	immunological	1599:1611	arg1	factors					1613:1619	the immunological factors	1595:1619	the immunological factors	1595:1619	Gut microbiota and derived metabolites interact with the immunological factors to improve intestinal epithelium barrier function.
36052065	2	43	theme	C57BL6	652:657	arg1	mice					664:667	C57BL6 male mice	652:667	C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	652:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	44	theme	cranberry	428:436	arg1	CP					451:452	CP	451:452	CP	451:452	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	44	theme	cranberry	428:436	arg1	polyphenols					438:448	cranberry polyphenols	428:448	cranberry polyphenols (CP)	428:453	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	12	45	theme	factor	2210:2215	arg1	recognition					2217:2227	dietary factor recognition	2202:2227	dietary factor recognition	2202:2227	The intestinal immune system was also shaped by dietary factor recognition.
36052065	1	46	theme	innate	304:309	arg1	responses					331:339	the innate and adaptive immune responses	300:339	the innate and adaptive immune responses associated with metabolic disorders	300:375	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36052065	14	47	theme	glucose	2586:2592	arg1	homeostasis					2594:2604	glucose homeostasis	2586:2604	glucose homeostasis	2586:2604	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	9	48	theme	Oral	1672:1675	arg1	administration					1677:1690	Oral administration	1672:1690	Oral administration of CP and AG to obese mice	1672:1717	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	2	49	theme	rich	456:459	arg1	CP					451:452	CP	451:452	CP	451:452	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	49	theme	rich	456:459	arg1	polyphenols					438:448	cranberry polyphenols	428:448	cranberry polyphenols (CP)	428:453	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	8	50	theme	intestinal	1632:1641	arg1	function					1662:1669	intestinal epithelium barrier function	1632:1669	intestinal epithelium barrier function	1632:1669	Gut microbiota and derived metabolites interact with the immunological factors to improve intestinal epithelium barrier function.
36052065	14	51	theme	integrity	2481:2489	arg1	repair					2452:2457	the repair	2448:2457	the repair of epithelial barrier integrity	2448:2489	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	0	52	theme	gut	47:49	arg1	response					58:65	gut immune response	47:65	gut immune response	47:65	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	1	53	theme	adaptive	315:322	arg1	responses					331:339	the innate and adaptive immune responses	300:339	the innate and adaptive immune responses associated with metabolic disorders	300:375	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36052065	8	54	theme	barrier	1654:1660	arg1	function					1662:1669	intestinal epithelium barrier function	1632:1669	intestinal epithelium barrier function	1632:1669	Gut microbiota and derived metabolites interact with the immunological factors to improve intestinal epithelium barrier function.
36052065	12	55	theme	dietary	2202:2208	arg1	recognition					2217:2227	dietary factor recognition	2202:2227	dietary factor recognition	2202:2227	The intestinal immune system was also shaped by dietary factor recognition.
36052065	14	56	theme	metabolic	2549:2557	arg1	inflammation					2559:2570	obesity-associated gut dysbiosis and metabolic inflammation	2512:2570	inflammation	2559:2570	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	2	57	from	effect	418:423	arg1	immunomodulation					632:647	mucosal immunomodulation	624:647	mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	624:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	57	from	effect	418:423	arg1	composition					584:594	gut microbiota composition	569:594	gut microbiota composition	569:594	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	57	from	effect	418:423	arg1	endotoxemia					607:617	metabolic endotoxemia	597:617	metabolic endotoxemia	597:617	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	57	from	effect	418:423	arg1	response					559:566	cardiometabolic response	543:566	cardiometabolic response	543:566	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	58	theme	present	382:388	arg1	study					390:394	The present study	378:394	The present study	378:394	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	7	59	contain	had	1390:1392	arg1	mice					1385:1388	CP+AG-supplemented HFHS-fed mice	1357:1388	CP+AG-supplemented HFHS-fed mice	1357:1388	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	7	59	contain	had	1390:1392	arg2	levels					1414:1419	significantly lower levels	1394:1419	significantly lower levels of plasma LBP	1394:1433	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	14	60	theme	mucosal	2420:2426	arg1	markers					2428:2434	key mucosal markers	2416:2434	key mucosal markers involved in the repair of epithelial barrier integrity	2416:2489	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	6	61	from	level	1272:1276	arg1	mice					1299:1302	obese mice	1293:1302	obese mice receiving AG	1293:1315	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	7	62	theme	intestinal	1513:1522	arg1	function					1532:1539	improved intestinal barrier function	1504:1539	improved intestinal barrier function	1504:1539	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	2	63	theme	microbiota	573:582	arg1	composition					584:594	gut microbiota composition	569:594	gut microbiota composition	569:594	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	10	64	theme	1ß	1949:1950	arg1	expression					1923:1932	the expression	1919:1932	the expression of interleukin 1ß (ILß1) in obese mice	1919:1971	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	0	65	theme	obesogenic	184:193	arg1	diet					195:198	an obesogenic diet	181:198	an obesogenic diet	181:198	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	1	66	theme	gut	266:268	arg1	microbiota					270:279	the gut microbiota	262:279	the gut microbiota	262:279	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36052065	7	67	theme	blunted	1470:1476	arg1	endotoxemia					1488:1498	blunted metabolic endotoxemia	1470:1498	blunted metabolic endotoxemia	1470:1498	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	6	68	theme	butyrate	1281:1288	arg1	level					1272:1276	a significantly increased level	1246:1276	a significantly increased level of butyrate in obese mice receiving AG	1246:1315	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	7	69	theme	metabolic	1478:1486	arg1	endotoxemia					1488:1498	blunted metabolic endotoxemia	1470:1498	blunted metabolic endotoxemia	1470:1498	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	14	70	theme	gut	2376:2378	arg1	composition					2391:2401	gut microbiota composition	2376:2401	gut microbiota composition	2376:2401	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	10	71	theme	toll-like	1856:1864	arg1	TLR					1876:1878	toll-like receptor (TLR)-2	1856:1881	toll-like receptor (TLR)-2 (Tlr2) expression	1856:1899	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	10	71	theme	toll-like	1856:1864	arg1	Tlr2					1884:1887	Tlr2	1884:1887	Tlr2	1884:1887	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	6	72	theme	glycan-degrading	1200:1215	arg1	bacteria					1217:1224	glycan-degrading bacteria	1200:1224	glycan-degrading bacteria	1200:1224	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	9	73	theme	immune	1762:1767	arg1	response					1769:1776	the pro-inflammatory immune response	1741:1776	the pro-inflammatory immune response	1741:1776	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	7	74	theme	HFHS-fed	1440:1447	arg1	controls					1449:1456	HFHS-fed controls	1440:1456	HFHS-fed controls	1440:1456	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	12	75	theme	immune	2169:2174	arg1	system					2176:2181	The intestinal immune system	2154:2181	The intestinal immune system	2154:2181	The intestinal immune system was also shaped by dietary factor recognition.
36052065	2	76	theme	agave-derived	512:524	arg1	neo-fructans					526:537	a highly branched agave-derived neo-fructans	494:537	a highly branched agave-derived neo-fructans	494:537	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	11	77	theme	receptor	2090:2097	arg1	domain					2112:2117	NOD-like receptor family pyrin domain	2081:2117	NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome	2081:2151	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	7	78	theme	LBP	1431:1433	arg1	levels					1414:1419	significantly lower levels	1394:1419	significantly lower levels of plasma LBP	1394:1433	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	6	79	theme	untreated	1333:1341	arg1	counterparts					1343:1354	untreated counterparts	1333:1354	untreated counterparts	1333:1354	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	9	80	theme	signaling	1796:1804	arg1	pathways					1806:1813	different signaling pathways	1786:1813	different signaling pathways	1786:1813	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	3	81	theme	CP+AG-fed	753:761	arg1	mice					763:766	CP+AG-fed mice	753:766	CP+AG-fed mice	753:766	Interestingly, CP+AG-fed mice had improved glucose homeostasis.
36052065	0	82	theme	glucose	150:156	arg1	metabolism					158:167	glucose metabolism	150:167	glucose metabolism	150:167	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	5	83	theme	glycan-degrading	1053:1068	arg1	bacteria					1070:1077	the glycan-degrading bacteria	1049:1077	the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens	1049:1180	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	13	84	theme	hydrocarbon	2310:2320	arg1	receptors					2322:2330	aryl hydrocarbon receptors	2305:2330	aryl hydrocarbon receptors (Ahr)	2305:2336	Indeed, the combination of CP+AG significantly increased the expression of aryl hydrocarbon receptors (Ahr).
36052065	13	84	theme	hydrocarbon	2310:2320	arg1	Ahr					2333:2335	Ahr	2333:2335	Ahr	2333:2335	Indeed, the combination of CP+AG significantly increased the expression of aryl hydrocarbon receptors (Ahr).
36052065	2	85	theme	cardiometabolic	543:557	arg1	response					559:566	cardiometabolic response	543:566	cardiometabolic response	543:566	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	7	86	theme	lower	1408:1412	arg1	levels					1414:1419	significantly lower levels	1394:1419	significantly lower levels of plasma LBP	1394:1433	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	2	87	theme	obesogenic	676:685	arg1	high-fat					687:694	an obesogenic high-fat	673:694	an obesogenic high-fat	673:694	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	87	theme	obesogenic	676:685	arg1	HFHS					714:717	HFHS	714:717	HFHS	714:717	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	11	88	theme	anti-inflammatory	2012:2028	arg1	Foxp3					2037:2041	the anti-inflammatory marker Foxp3	2008:2041	the anti-inflammatory marker Foxp3	2008:2041	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	4	89	theme	relative	882:889	arg1	abundance					891:899	the relative abundance	878:899	the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health	878:982	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	6	90	theme	bacteria	1217:1224	arg1	increase					1188:1195	This increase	1183:1195	This increase of glycan-degrading bacteria	1183:1224	This increase of glycan-degrading bacteria was consistent with a significantly increased level of butyrate in obese mice receiving AG, as compared to untreated counterparts.
36052065	3	91	theme	glucose	781:787	arg1	homeostasis					789:799	glucose homeostasis	781:799	glucose homeostasis	781:799	Interestingly, CP+AG-fed mice had improved glucose homeostasis.
36052065	11	92	theme	family	2099:2104	arg1	domain					2112:2117	NOD-like receptor family pyrin domain	2081:2117	NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome	2081:2151	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	2	93	theme	agavins	480:486	arg1	effect					418:423	the effect	414:423	the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	414:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	7	94	theme	HFHS-fed	1376:1383	arg1	mice					1385:1388	CP+AG-supplemented HFHS-fed mice	1357:1388	CP+AG-supplemented HFHS-fed mice	1357:1388	CP+AG-supplemented HFHS-fed mice had significantly lower levels of plasma LBP than HFHS-fed controls, suggesting blunted metabolic endotoxemia and improved intestinal barrier function.
36052065	11	95	theme	pyrin	2106:2110	arg1	domain					2112:2117	NOD-like receptor family pyrin domain	2081:2117	NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome	2081:2151	Moreover, AG selectively promoted the anti-inflammatory marker Foxp3, while CP increased the expression of NOD-like receptor family pyrin domain containing 6 (Nlrp6) inflammasome.
36052065	8	96	theme	derived	1561:1567	arg1	metabolites					1569:1579	derived metabolites	1561:1579	derived metabolites	1561:1579	Gut microbiota and derived metabolites interact with the immunological factors to improve intestinal epithelium barrier function.
36052065	1	97	theme	metabolic	357:365	arg1	disorders					367:375	metabolic disorders	357:375	metabolic disorders	357:375	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36052065	2	98	from	rich	456:459	arg1	flavonoids					464:473	flavonoids	464:473	flavonoids	464:473	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	99	theme	mucosal	624:630	arg1	immunomodulation					632:647	mucosal immunomodulation	624:647	mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	624:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	0	100	theme	barrier	114:120	arg1	function					122:129	gut barrier function	110:129	gut barrier function	110:129	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	2	101	from	flavonoids	464:473	arg1	rich					456:459	rich	456:459	rich	456:459	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	4	102	dep	and	843:845	arg1	five-fold					857:865	five-fold	857:865	five-fold	857:865	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	2	103	theme	branched	503:510	arg1	neo-fructans					526:537	a highly branched agave-derived neo-fructans	494:537	a highly branched agave-derived neo-fructans	494:537	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	2	104	theme	male	659:662	arg1	mice					664:667	C57BL6 male mice	652:667	C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	652:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	0	105	theme	agave	26:30	arg1	agavins					32:38	agave agavins	26:38	agave agavins	26:38	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	13	106	theme	CP+AG	2257:2261	arg1	combination					2242:2252	the combination	2238:2252	the combination of CP+AG	2238:2261	Indeed, the combination of CP+AG significantly increased the expression of aryl hydrocarbon receptors (Ahr).
36052065	2	107	theme	polyphenols	438:448	arg1	effect					418:423	the effect	414:423	the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks	414:735	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	8	108	theme	epithelium	1643:1652	arg1	function					1662:1669	intestinal epithelium barrier function	1632:1669	intestinal epithelium barrier function	1632:1669	Gut microbiota and derived metabolites interact with the immunological factors to improve intestinal epithelium barrier function.
36052065	2	109	theme	metabolic	597:605	arg1	endotoxemia					607:617	metabolic endotoxemia	597:617	metabolic endotoxemia	597:617	The present study aimed to evaluate the effect of cranberry polyphenols (CP), rich in flavonoids, and agavins (AG), a highly branched agave-derived neo-fructans, on cardiometabolic response, gut microbiota composition, metabolic endotoxemia, and mucosal immunomodulation of C57BL6 male mice fed an obesogenic high-fat and high-sucrose (HFHS) diet for 9 weeks.
36052065	1	110	theme	bioactive	232:240	arg1	compounds					242:250	plant-based bioactive compounds	220:250	plant-based bioactive compounds	220:250	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36052065	14	111	theme	epithelial	2462:2471	arg1	integrity					2481:2489	epithelial barrier integrity	2462:2489	epithelial barrier integrity	2462:2489	Altogether, both CP and AG can shape gut microbiota composition and regulate key mucosal markers involved in the repair of epithelial barrier integrity, thereby attenuating obesity-associated gut dysbiosis and metabolic inflammation and improving glucose homeostasis.
36052065	0	112	theme	immune	51:56	arg1	response					58:65	gut immune response	47:65	gut immune response	47:65	Cranberry polyphenols and agave agavins impact gut immune response and microbiota composition while improving gut barrier function, inflammation, and glucose metabolism in mice fed an obesogenic diet.
36052065	8	113	link	derived	1561:1567	arg1	metabolites					1569:1579	derived metabolites	1561:1579	derived metabolites	1561:1579	Gut microbiota and derived metabolites interact with the immunological factors to improve intestinal epithelium barrier function.
36052065	4	114	theme	muciniphila	916:926	arg1	abundance					891:899	the relative abundance	878:899	the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health	878:982	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	5	115	dep	bacteria	1070:1077	arg1	intestinale					1091:1101	Muribaculum intestinale	1079:1101	Muribaculum intestinale	1079:1101	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	5	115	dep	bacteria	1070:1077	arg1	rodentium					1119:1127	Faecalibaculum rodentium	1104:1127	Faecalibaculum rodentium	1104:1127	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	5	115	dep	bacteria	1070:1077	arg1	uniformis					1142:1150	Bacteroides uniformis	1130:1150	Bacteroides uniformis	1130:1150	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	5	115	dep	bacteria	1070:1077	arg1	acidifaciens					1169:1180	Bacteroides acidifaciens	1157:1180	Bacteroides acidifaciens	1157:1180	AG, either alone or combined with CP (CP+AG), mainly stimulated the glycan-degrading bacteria Muribaculum intestinale, Faecalibaculum rodentium, Bacteroides uniformis, and Bacteroides acidifaciens.
36052065	10	116	theme	interleukin	1937:1947	arg1	ILß1					1953:1956	ILß1	1953:1956	ILß1	1953:1956	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	10	116	theme	interleukin	1937:1947	arg1	1ß					1949:1950	interleukin 1ß	1937:1950	interleukin 1ß (ILß1)	1937:1957	CP and AG, alone or combined, increased toll-like receptor (TLR)-2 (Tlr2) expression, while decreasing the expression of interleukin 1ß (ILß1) in obese mice.
36052065	4	117	theme	Oral	802:805	arg1	supplementation					807:821	Oral supplementation	802:821	Oral supplementation with CP	802:829	Oral supplementation with CP selectively and robustly (five-fold) increases the relative abundance of Akkermansia muciniphila, a beneficial bacteria associated with metabolic health.
36052065	1	118	theme	immune	324:329	arg1	responses					331:339	the innate and adaptive immune responses	300:339	the innate and adaptive immune responses associated with metabolic disorders	300:375	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36052065	9	119	theme	CP	1695:1696	arg1	administration					1677:1690	Oral administration	1672:1690	Oral administration of CP and AG to obese mice	1672:1717	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	1	120	theme	plant-based	220:230	arg1	compounds					242:250	plant-based bioactive compounds	220:250	plant-based bioactive compounds	220:250	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36052065	9	121	theme	AG	1702:1703	arg1	administration					1677:1690	Oral administration	1672:1690	Oral administration of CP and AG to obese mice	1672:1717	Oral administration of CP and AG to obese mice contributed to dampen the pro-inflammatory immune response through different signaling pathways.
36052065	1	122	theme	compounds	242:250	arg1	consumption					205:215	The consumption	201:215	The consumption of plant-based bioactive compounds	201:250	The consumption of plant-based bioactive compounds modulates the gut microbiota and interacts with the innate and adaptive immune responses associated with metabolic disorders.
36606411	11	0	dep	CONCLUSIONS	1595:1605	arg1	indicates					1610:1618	indicates	1610:1618	indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone	1610:1790	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	3	1	with	salts	498:502	arg1	derivatives					530:540	various polysiloxane derivatives	509:540	various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	509:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	11	2	theme	anti-scar	1709:1717	arg1	activity					1719:1726	anti-scar activity	1709:1726	anti-scar activity	1709:1726	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	5	3	theme	phase	871:875	arg1	dispersion					877:886	aqueous phase dispersion	863:886	aqueous phase dispersion	863:886	The method of preparation comprises mixing of aqueous phase dispersion and polysiloxanes blend under stirring at room temperature.
36606411	8	4	theme	formulation	1204:1214	arg1	system					1216:1221	the developed formulation system	1190:1221	the developed formulation system	1190:1221	It was found that the developed formulation system consists of desirable attributes for skin applications.
36606411	7	5	theme	physicochemical	1084:1098	arg1	rheology					1112:1119	rheology	1112:1119	rheology	1112:1119	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	7	5	theme	physicochemical	1084:1098	arg1	properties					1100:1109	physicochemical properties	1084:1109	physicochemical properties	1084:1109	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	7	5	theme	physicochemical	1084:1098	arg1	activity					1147:1154	anti-scar activity	1137:1154	anti-scar activity	1137:1154	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	7	5	theme	physicochemical	1084:1098	arg1	stability					1122:1130	stability	1122:1130	stability	1122:1130	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	2	6	theme	silicone	223:230	arg1	gel					232:234	silicone gel	223:234	silicone gel	223:234	It shows that silicone gel is effective in preventing and improving scars formed due to a wound formation after injury.
36606411	7	7	theme	anti-scar	1137:1145	arg1	activity					1147:1154	anti-scar activity	1137:1154	anti-scar activity	1137:1154	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	7	7	theme	anti-scar	1137:1145	arg1	properties					1100:1109	physicochemical properties	1084:1109	physicochemical properties	1084:1109	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	6	8	dep	RESULTS	948:954	arg1	results					959:965	results	959:965	results in the formation of a homogenous smooth gel formulation	959:1021	RESULTS It results in the formation of a homogenous smooth gel formulation.
36606411	3	9	theme	viscosity	586:594	arg1	range					596:600	desired viscosity range	578:600	desired viscosity range	578:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	7	10	theme	topical	1038:1044	arg1	formulation					1050:1060	The developed topical gel formulation	1024:1060	The developed topical gel formulation	1024:1060	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	5	11	theme	room	930:933	arg1	temperature					935:945	room temperature	930:945	room temperature	930:945	The method of preparation comprises mixing of aqueous phase dispersion and polysiloxanes blend under stirring at room temperature.
36606411	0	12	theme	skin	61:64	arg1	design					84:89	skin scar: Formulation design	61:89	skin scar: Formulation design	61:89	Topical gel containing Polysiloxanes and hyaluronic acid for skin scar: Formulation design, characterization, and In vivo activity.
36606411	3	13	dep	proportion	556:565	arg1	achieve					570:576	achieve	570:576	to achieve desired viscosity range	567:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	6	14	theme	gel	1007:1009	arg1	formulation					1011:1021	a homogenous smooth gel formulation	987:1021	a homogenous smooth gel formulation	987:1021	RESULTS It results in the formation of a homogenous smooth gel formulation.
36606411	10	15	theme	healed	1481:1486	arg1	tissues					1493:1499	healed skin tissues	1481:1499	healed skin tissues	1481:1499	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	3	16	from	derivatives	530:540	arg1	proportion					556:565	a specific proportion	545:565	a specific proportion to achieve desired viscosity range	545:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	9	17	theme	polysiloxane	1314:1325	arg1	formulation					1331:1341	developed polysiloxane gel formulation	1304:1341	developed polysiloxane gel formulation for anti-scar activity	1304:1364	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	11	18	contain	containing	1761:1770	arg1	product					1753:1759	the marketed product	1740:1759	the marketed product containing polysiloxanes alone	1740:1790	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	11	18	contain	containing	1761:1770	arg2	polysiloxanes					1772:1784	polysiloxanes	1772:1784	polysiloxanes alone	1772:1790	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	6	19	theme	homogenous	989:998	arg1	formulation					1011:1021	a homogenous smooth gel formulation	987:1021	a homogenous smooth gel formulation	987:1021	RESULTS It results in the formation of a homogenous smooth gel formulation.
36606411	9	20	theme	formulation	1331:1341	arg1	investigation					1287:1299	In vivo investigation	1279:1299	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity	1279:1364	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	4	21	theme	hyaluronate	769:779	arg1	combination					734:744	a combination	732:744	a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives	732:814	METHODS We have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives.
36606411	9	22	theme	anti-scar	1347:1355	arg1	activity					1357:1364	anti-scar activity	1347:1364	anti-scar activity	1347:1364	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	0	23	theme	hyaluronic	41:50	arg1	acid					52:55	hyaluronic acid	41:55	hyaluronic acid	41:55	Topical gel containing Polysiloxanes and hyaluronic acid for skin scar: Formulation design, characterization, and In vivo activity.
36606411	3	24	theme	silicone	374:381	arg1	composition					387:397	a silicone gel composition	372:397	a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	372:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	10	25	theme	tissues	1493:1499	arg1	study					1472:1476	a histopathology study	1455:1476	a histopathology study of healed skin tissues	1455:1499	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	3	26	theme	novel	410:414	arg1	concept					416:422	a novel concept	408:422	a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	408:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	0	27	theme	Topical	0:6	arg1	gel					8:10	Topical gel	0:10	Topical gel	0:10	Topical gel containing Polysiloxanes and hyaluronic acid for skin scar: Formulation design, characterization, and In vivo activity.
36606411	10	28	theme	microscopic	1531:1541	arg1	structures					1548:1557	all microscopic skin structures	1527:1557	all microscopic skin structures	1527:1557	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	3	29	theme	active	451:456	arg1	material					458:465	a biologically active material	436:465	a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	436:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	4	30	theme	derivatives	804:814	arg1	combination					734:744	a combination	732:744	a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives	732:814	METHODS We have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives.
36606411	11	31	from	improvement	1694:1704	arg1	activity					1719:1726	anti-scar activity	1709:1726	anti-scar activity	1709:1726	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	3	32	dep	OBJECTIVES	329:338	arg1	investigates					351:362	investigates	351:362	investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury	351:679	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	3	33	theme	beneficial	637:646	arg1	effect					648:653	a synergistic beneficial effect	623:653	a synergistic beneficial effect	623:653	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	3	34	theme	hyaluronic	475:484	arg1	acid					486:489	hyaluronic acid	475:489	hyaluronic acid	475:489	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	10	35	theme	structures	1548:1557	arg1	formation					1514:1522	the formation	1510:1522	the formation of all microscopic skin structures	1510:1557	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	4	36	theme	polysiloxane	782:793	arg1	combination					734:744	a combination	732:744	a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives	732:814	METHODS We have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives.
36606411	9	37	theme	Kelo-cote	1421:1429	arg1	gel					1436:1438	Kelo-cote scar gel	1421:1438	Kelo-cote scar gel	1421:1438	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	9	37	theme	Kelo-cote	1421:1429	arg1	product					1412:1418	marketed product	1403:1418	marketed product (Kelo-cote scar gel)	1403:1439	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	1	38	theme	BACKGROUND	132:141	arg1	formation					148:156	BACKGROUND Scar formation	132:156	BACKGROUND Scar formation	132:156	BACKGROUND Scar formation is undesirable both cosmetically and functionally.
36606411	11	39	theme	hyaluronate	1669:1679	arg1	combination					1629:1639	the combination	1625:1639	the combination of polysiloxanes and sodium hyaluronate	1625:1679	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	3	40	contain	has	619:621	arg1	action					612:617	their action	606:617	their action	606:617	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	3	40	contain	has	619:621	arg1	composition					387:397	a silicone gel composition	372:397	a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	372:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	3	40	contain	has	619:621	arg2	effect					648:653	a synergistic beneficial effect	623:653	a synergistic beneficial effect	623:653	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	10	41	theme	Kelo-cote	1575:1583	arg1	gel					1590:1592	the Kelo-cote scar gel	1571:1592	the Kelo-cote scar gel	1571:1592	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	11	42	theme	marketed	1744:1751	arg1	product					1753:1759	the marketed product	1740:1759	the marketed product containing polysiloxanes alone	1740:1790	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	1	43	theme	Scar	143:146	arg1	formation					148:156	BACKGROUND Scar formation	132:156	BACKGROUND Scar formation	132:156	BACKGROUND Scar formation is undesirable both cosmetically and functionally.
36606411	9	44	dep	In	1279:1280	arg1	vivo					1282:1285	vivo	1282:1285	vivo	1282:1285	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	3	45	theme	polysiloxane	517:528	arg1	derivatives					530:540	various polysiloxane derivatives	509:540	various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	509:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	2	46	theme	wound	299:303	arg1	formation					305:313	a wound formation	297:313	a wound formation after injury	297:326	It shows that silicone gel is effective in preventing and improving scars formed due to a wound formation after injury.
36606411	5	47	theme	aqueous	863:869	arg1	dispersion					877:886	aqueous phase dispersion	863:886	aqueous phase dispersion	863:886	The method of preparation comprises mixing of aqueous phase dispersion and polysiloxanes blend under stirring at room temperature.
36606411	8	48	theme	skin	1260:1263	arg1	applications					1265:1276	skin applications	1260:1276	skin applications	1260:1276	It was found that the developed formulation system consists of desirable attributes for skin applications.
36606411	0	49	theme	In	114:115	arg1	activity					122:129	In vivo activity	114:129	In vivo activity	114:129	Topical gel containing Polysiloxanes and hyaluronic acid for skin scar: Formulation design, characterization, and In vivo activity.
36606411	3	50	theme	specific	547:554	arg1	proportion					556:565	a specific proportion	545:565	a specific proportion to achieve desired viscosity range	545:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	8	51	theme	developed	1194:1202	arg1	system					1216:1221	the developed formulation system	1190:1221	the developed formulation system	1190:1221	It was found that the developed formulation system consists of desirable attributes for skin applications.
36606411	5	52	theme	preparation	831:841	arg1	method					821:826	The method	817:826	The method of preparation	817:841	The method of preparation comprises mixing of aqueous phase dispersion and polysiloxanes blend under stirring at room temperature.
36606411	7	53	theme	gel	1046:1048	arg1	formulation					1050:1060	The developed topical gel formulation	1024:1060	The developed topical gel formulation	1024:1060	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	3	54	theme	desired	578:584	arg1	range					596:600	desired viscosity range	578:600	desired viscosity range	578:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	7	55	theme	developed	1028:1036	arg1	formulation					1050:1060	The developed topical gel formulation	1024:1060	The developed topical gel formulation	1024:1060	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	9	56	theme	In	1279:1280	arg1	investigation					1287:1299	In vivo investigation	1279:1299	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity	1279:1364	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	4	57	theme	topical	710:716	arg1	gel					718:720	a topical gel	708:720	a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives	708:814	METHODS We have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives.
36606411	0	58	theme	scar	66:69	arg1	design					84:89	skin scar: Formulation design	61:89	skin scar: Formulation design	61:89	Topical gel containing Polysiloxanes and hyaluronic acid for skin scar: Formulation design, characterization, and In vivo activity.
36606411	5	59	theme	dispersion	877:886	arg1	mixing					853:858	mixing	853:858	mixing of aqueous phase dispersion	853:886	The method of preparation comprises mixing of aqueous phase dispersion and polysiloxanes blend under stirring at room temperature.
36606411	6	60	theme	formulation	1011:1021	arg1	formation					974:982	the formation	970:982	the formation of a homogenous smooth gel formulation	970:1021	RESULTS It results in the formation of a homogenous smooth gel formulation.
36606411	7	61	from	activity	1147:1154	arg1	rats					1166:1169	Wistar rats	1159:1169	Wistar rats	1159:1169	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	0	62	theme	Formulation	72:82	arg1	design					84:89	skin scar: Formulation design	61:89	skin scar: Formulation design	61:89	Topical gel containing Polysiloxanes and hyaluronic acid for skin scar: Formulation design, characterization, and In vivo activity.
36606411	9	63	theme	developed	1304:1312	arg1	formulation					1331:1341	developed polysiloxane gel formulation	1304:1341	developed polysiloxane gel formulation for anti-scar activity	1304:1364	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	0	64	dep	In	114:115	arg1	vivo					117:120	vivo	117:120	vivo	117:120	Topical gel containing Polysiloxanes and hyaluronic acid for skin scar: Formulation design, characterization, and In vivo activity.
36606411	6	65	theme	smooth	1000:1005	arg1	formulation					1011:1021	a homogenous smooth gel formulation	987:1021	a homogenous smooth gel formulation	987:1021	RESULTS It results in the formation of a homogenous smooth gel formulation.
36606411	9	66	theme	gel	1327:1329	arg1	formulation					1331:1341	developed polysiloxane gel formulation	1304:1341	developed polysiloxane gel formulation for anti-scar activity	1304:1364	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	10	67	theme	histopathology	1457:1470	arg1	study					1472:1476	a histopathology study	1455:1476	a histopathology study of healed skin tissues	1455:1499	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	4	68	theme	emulsifiers	749:759	arg1	combination					734:744	a combination	732:744	a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives	732:814	METHODS We have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives.
36606411	4	69	dep	METHODS	682:688	arg1	developed					698:706	developed	698:706	have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives	693:814	METHODS We have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives.
36606411	4	70	theme	sodium	762:767	arg1	hyaluronate					769:779	sodium hyaluronate	762:779	sodium hyaluronate	762:779	METHODS We have developed a topical gel utilizing a combination of emulsifiers, sodium hyaluronate, polysiloxane, and its derivatives.
36606411	3	71	theme	gel	383:385	arg1	composition					387:397	a silicone gel composition	372:397	a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	372:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	10	72	theme	skin	1488:1491	arg1	tissues					1493:1499	healed skin tissues	1481:1499	healed skin tissues	1481:1499	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	9	73	theme	promising	1372:1380	arg1	outcomes					1382:1389	promising outcomes	1372:1389	promising outcomes	1372:1389	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	7	74	from	rheology	1112:1119	arg1	rats					1166:1169	Wistar rats	1159:1169	Wistar rats	1159:1169	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	7	75	theme	Wistar	1159:1164	arg1	rats					1166:1169	Wistar rats	1159:1169	Wistar rats	1159:1169	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	7	76	from	stability	1122:1130	arg1	rats					1166:1169	Wistar rats	1159:1169	Wistar rats	1159:1169	The developed topical gel formulation was characterized for physicochemical properties, rheology, stability, and anti-scar activity in Wistar rats.
36606411	3	77	theme	synergistic	625:635	arg1	effect					648:653	a synergistic beneficial effect	623:653	a synergistic beneficial effect	623:653	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	9	78	theme	marketed	1403:1410	arg1	gel					1436:1438	Kelo-cote scar gel	1421:1438	Kelo-cote scar gel	1421:1438	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	9	78	theme	marketed	1403:1410	arg1	product					1412:1418	marketed product	1403:1418	marketed product (Kelo-cote scar gel)	1403:1439	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	10	79	theme	skin	1543:1546	arg1	structures					1548:1557	all microscopic skin structures	1527:1557	all microscopic skin structures	1527:1557	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	3	80	theme	skin	658:661	arg1	scar					663:666	skin scar	658:666	skin scar after injury	658:679	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36606411	11	81	theme	sodium	1662:1667	arg1	hyaluronate					1669:1679	sodium hyaluronate	1662:1679	sodium hyaluronate	1662:1679	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	8	82	theme	desirable	1235:1243	arg1	attributes					1245:1254	desirable attributes	1235:1254	desirable attributes for skin applications	1235:1276	It was found that the developed formulation system consists of desirable attributes for skin applications.
36606411	9	83	theme	scar	1431:1434	arg1	gel					1436:1438	Kelo-cote scar gel	1421:1438	Kelo-cote scar gel	1421:1438	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	9	83	theme	scar	1431:1434	arg1	product					1412:1418	marketed product	1403:1418	marketed product (Kelo-cote scar gel)	1403:1439	In vivo investigation of developed polysiloxane gel formulation for anti-scar activity shown promising outcomes compared to marketed product (Kelo-cote scar gel).
36606411	10	84	theme	scar	1585:1588	arg1	gel					1590:1592	the Kelo-cote scar gel	1571:1592	the Kelo-cote scar gel	1571:1592	Furthermore, a histopathology study of healed skin tissues observed the formation of all microscopic skin structures compared to the Kelo-cote scar gel.
36606411	11	85	theme	polysiloxanes	1644:1656	arg1	combination					1629:1639	the combination	1625:1639	the combination of polysiloxanes and sodium hyaluronate	1625:1679	CONCLUSIONS It indicates that the combination of polysiloxanes and sodium hyaluronate resulting an improvement in anti-scar activity compared to the marketed product containing polysiloxanes alone.
36606411	3	86	theme	various	509:515	arg1	derivatives					530:540	various polysiloxane derivatives	509:540	various polysiloxane derivatives in a specific proportion to achieve desired viscosity range	509:600	OBJECTIVES This study investigates whether a silicone gel composition based on a novel concept of infusing a biologically active material such as hyaluronic acid and/or salts with various polysiloxane derivatives in a specific proportion to achieve desired viscosity range and their action has a synergistic beneficial effect on skin scar after injury.
36365725	0	0	theme	Kinetic	80:86	arg1	Assessment					88:97	A Kinetic Assessment	78:97	A Kinetic Assessment	78:97	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	3	1	theme	Arrhenius-type	756:769	arg1	dependence					783:792	an Arrhenius-type temperature dependence	753:792	an Arrhenius-type temperature dependence	753:792	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	5	2	theme	key	988:990	arg1	information					992:1002	key information	988:1002	key information	988:1002	The kinetic equations allowed a quantitative assessment of the experimental results, providing key information for process simulation and evaluation.
36365725	2	3	from	treatments	315:324	arg1	phases					303:308	The liquid phases	292:308	The liquid phases from treatments	292:324	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	0	4	from	Effects	0:6	arg1	Polysaccharides					61:75	Miscanthus × giganteus Polysaccharides	38:75	Miscanthus × giganteus Polysaccharides	38:75	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	3	5	theme	acetic	570:575	arg1	acid					577:580	acetic acid	570:580	acetic acid	570:580	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	2	6	theme	liquid	296:301	arg1	phases					303:308	The liquid phases	292:308	The liquid phases from treatments	292:324	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	5	7	theme	quantitative	925:936	arg1	assessment					938:947	a quantitative assessment	923:947	a quantitative assessment of the experimental results	923:975	The kinetic equations allowed a quantitative assessment of the experimental results, providing key information for process simulation and evaluation.
36365725	3	8	theme	target	515:520	arg1	compounds					522:530	the target compounds	511:530	the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products)	511:614	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	5	9	theme	experimental	956:967	arg1	results					969:975	the experimental results	952:975	the experimental results	952:975	The kinetic equations allowed a quantitative assessment of the experimental results, providing key information for process simulation and evaluation.
36365725	3	10	theme	temperature	771:781	arg1	dependence					783:792	an Arrhenius-type temperature dependence	753:792	an Arrhenius-type temperature dependence	753:792	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	5	11	dep	process	1008:1014	arg1	simulation					1016:1025	simulation	1016:1025	simulation	1016:1025	The kinetic equations allowed a quantitative assessment of the experimental results, providing key information for process simulation and evaluation.
36365725	0	12	dep	Assessment	88:97	arg1	Effects					0:6	Effects	0:6	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides	0:75	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	1	13	theme	hot	183:185	arg1	water					198:202	hot compressed water	183:202	hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C	183:289	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	4	14	theme	activation	813:822	arg1	energies					824:831	The corresponding activation energies	795:831	The corresponding activation energies	795:831	The corresponding activation energies were calculated and compared with data from the literature.
36365725	1	15	theme	compressed	187:196	arg1	water					198:202	hot compressed water	183:202	hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C	183:289	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	3	16	theme	sugar-decomposition	586:604	arg1	products					606:613	sugar-decomposition products	586:613	sugar-decomposition products	586:613	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	0	17	theme	Processing	24:33	arg1	Effects					0:6	Effects	0:6	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides	0:75	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	4	18	theme	corresponding	799:811	arg1	energies					824:831	The corresponding activation energies	795:831	The corresponding activation energies	795:831	The corresponding activation energies were calculated and compared with data from the literature.
36365725	2	19	theme	polysaccharides	379:393	arg1	breakdown					354:362	the breakdown	350:362	the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products	350:453	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	2	20	theme	products	446:453	arg1	scope					402:406	a scope	400:406	a scope of soluble intermediates and reaction products	400:453	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	1	21	theme	°C	288:289	arg1	range					271:275	the range	267:275	the range of 190-240 °C	267:289	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	1	22	theme	Miscanthus	100:109	arg1	samples					123:129	Miscanthus × giganteus samples	100:129	Miscanthus × giganteus samples	100:129	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	2	23	theme	reaction	437:444	arg1	products					446:453	reaction products	437:453	reaction products	437:453	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	1	24	theme	×	111:111	arg1	samples					123:129	Miscanthus × giganteus samples	100:129	Miscanthus × giganteus samples	100:129	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	2	25	theme	intermediates	419:431	arg1	scope					402:406	a scope	400:406	a scope of soluble intermediates and reaction products	400:453	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	0	26	theme	Miscanthus	38:47	arg1	Polysaccharides					61:75	Miscanthus × giganteus Polysaccharides	38:75	Miscanthus × giganteus Polysaccharides	38:75	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	3	27	theme	experimental	460:471	arg1	profiles					487:494	The experimental concentration profiles	456:494	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products)	456:614	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	5	28	theme	results	969:975	arg1	assessment					938:947	a quantitative assessment	923:947	a quantitative assessment of the experimental results	923:975	The kinetic equations allowed a quantitative assessment of the experimental results, providing key information for process simulation and evaluation.
36365725	2	29	theme	soluble	411:417	arg1	intermediates					419:431	soluble intermediates	411:431	soluble intermediates	411:431	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	1	30	dep	water	198:202	arg1	hydrothermal					205:216	hydrothermal	205:216	hydrothermal	205:216	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	1	30	dep	water	198:202	arg1	treatments					236:245	autohydrolysis treatments	221:245	autohydrolysis treatments	221:245	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	1	31	theme	autohydrolysis	221:234	arg1	treatments					236:245	autohydrolysis treatments	221:245	autohydrolysis treatments	221:245	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	0	32	theme	giganteus	51:59	arg1	Polysaccharides					61:75	Miscanthus × giganteus Polysaccharides	38:75	Miscanthus × giganteus Polysaccharides	38:75	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	1	33	theme	giganteus	113:121	arg1	samples					123:129	Miscanthus × giganteus samples	100:129	Miscanthus × giganteus samples	100:129	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	0	34	theme	×	49:49	arg1	Polysaccharides					61:75	Miscanthus × giganteus Polysaccharides	38:75	Miscanthus × giganteus Polysaccharides	38:75	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	1	35	from	temperatures	251:262	arg1	water					198:202	hot compressed water	183:202	hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C	183:289	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	1	35	from	temperatures	251:262	arg1	range					271:275	the range	267:275	the range of 190-240 °C	267:289	Miscanthus × giganteus samples were characterized for composition and treated with hot compressed water (hydrothermal or autohydrolysis treatments) at temperatures in the range of 190-240 °C.
36365725	5	36	theme	kinetic	897:903	arg1	equations					905:913	The kinetic equations	893:913	The kinetic equations	893:913	The kinetic equations allowed a quantitative assessment of the experimental results, providing key information for process simulation and evaluation.
36365725	3	37	theme	kinetic	659:665	arg1	mechanism					667:675	a pseudohomogeneous kinetic mechanism	639:675	a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence	639:792	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	3	38	theme	kinetic	724:730	arg1	coefficients					732:743	kinetic coefficients	724:743	kinetic coefficients showing an Arrhenius-type temperature dependence	724:792	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	3	39	dep	compounds	522:530	arg1	monosaccharides					533:547	monosaccharides	533:547	monosaccharides	533:547	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	3	39	dep	compounds	522:530	arg1	saccharides					557:567	higher saccharides	550:567	higher saccharides	550:567	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	3	39	dep	compounds	522:530	arg1	acid					577:580	acetic acid	570:580	acetic acid	570:580	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	3	39	dep	compounds	522:530	arg1	products					606:613	sugar-decomposition products	586:613	sugar-decomposition products	586:613	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	4	40	from	literature	881:890	arg1	data					867:870	data	867:870	data from the literature	867:890	The corresponding activation energies were calculated and compared with data from the literature.
36365725	3	41	theme	concentration	473:485	arg1	profiles					487:494	The experimental concentration profiles	456:494	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products)	456:614	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	3	42	theme	pseudohomogeneous	641:657	arg1	mechanism					667:675	a pseudohomogeneous kinetic mechanism	639:675	a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence	639:792	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
36365725	2	43	theme	susceptible	367:377	arg1	polysaccharides					379:393	susceptible polysaccharides	367:393	susceptible polysaccharides	367:393	The liquid phases from treatments were analyzed to assess the breakdown of susceptible polysaccharides into a scope of soluble intermediates and reaction products.
36365725	0	44	theme	Hydrothermal	11:22	arg1	Processing					24:33	Hydrothermal Processing	11:33	Hydrothermal Processing	11:33	Effects of Hydrothermal Processing on Miscanthus × giganteus Polysaccharides: A Kinetic Assessment.
36365725	3	45	theme	higher	550:555	arg1	saccharides					557:567	higher saccharides	550:567	higher saccharides	550:567	The experimental concentration profiles determined for the target compounds (monosaccharides, higher saccharides, acetic acid and sugar-decomposition products) were interpreted using a pseudohomogeneous kinetic mechanism involving 27 reactions, which were governed by kinetic coefficients showing an Arrhenius-type temperature dependence.
35342881	3	0	theme	fecal	723:727	arg1	fermentation					729:740	the fecal fermentation	719:740	the fecal fermentation	719:740	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	1	1	theme	dynamic	159:165	arg1	variations					167:176	dynamic variations	159:176	dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	159:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	4	2	theme	intestinal	921:930	arg1	microbes					932:939	profitable intestinal microbes	910:939	profitable intestinal microbes	910:939	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	4	2	theme	intestinal	921:930	arg1	Lactococcus					963:973	Lactococcus	963:973	Lactococcus	963:973	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	4	2	theme	intestinal	921:930	arg1	Bifidobacterium					980:994	Bifidobacterium	980:994	Bifidobacterium	980:994	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	4	2	theme	intestinal	921:930	arg1	Bacteroides					950:960	Bacteroides	950:960	Bacteroides	950:960	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	0	3	theme	simulated	91:99	arg1	digestion					101:109	simulated digestion	91:109	simulated digestion	91:109	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	0	4	from	variations	8:17	arg1	characteristics					38:52	physicochemical characteristics	22:52	physicochemical characteristics of oolong tea polysaccharides	22:82	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	0	5	theme	fecal	115:119	arg1	fermentation					121:132	fecal fermentation	115:132	fecal fermentation	115:132	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	1	6	from	variations	167:176	arg1	characteristics					197:211	physicochemical characteristics	181:211	physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	181:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	3	7	theme	indigestible	558:569	arg1	WYP					571:573	the indigestible WYP	554:573	the indigestible WYP	554:573	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	1	8	from	stages	315:320	arg1	tea					249:251	'Wuyi rock' tea	237:251	'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	237:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	8	from	stages	315:320	arg1	WYP					254:256	WYP	254:256	WYP	254:256	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	3	9	theme	WYP	571:573	arg1	composition					611:621	chemical composition	602:621	chemical composition	602:621	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	9	theme	WYP	571:573	arg1	weight					662:667	molecular weight	652:667	molecular weight	652:667	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	9	theme	WYP	571:573	arg1	characteristics					535:549	physicochemical characteristics	519:549	physicochemical characteristics	519:549	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	9	theme	WYP	571:573	arg1	sugar					595:599	reducing sugar	586:599	reducing sugar	586:599	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	9	theme	WYP	571:573	arg1	monosaccharide					636:649	constituent monosaccharide	624:649	constituent monosaccharide	624:649	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	9	theme	WYP	571:573	arg1	spectrum					680:687	FT-IR spectrum	674:687	FT-IR spectrum	674:687	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	1	10	theme	different	262:270	arg1	digestion					282:290	different simulated digestion and fecal fermentation stages	262:320	digestion	282:290	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	4	11	theme	microbial	853:861	arg1	composition					863:873	the microbial composition	849:873	the microbial composition	849:873	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	1	12	theme	physicochemical	181:195	arg1	characteristics					197:211	physicochemical characteristics	181:211	physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	181:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	6	13	theme	intestinal	1263:1272	arg1	health					1274:1279	intestinal health	1263:1279	intestinal health	1263:1279	The results showed that WYP was slightly digested in the gastrointestinal tract in vitro, but could be obviously utilized by intestinal microbiota, and might possess the potential to improve intestinal health.
35342881	6	14	contain	possess	1230:1236	arg2	potential					1242:1250	the potential to improve intestinal health	1238:1279	the potential to improve intestinal health	1238:1279	The results showed that WYP was slightly digested in the gastrointestinal tract in vitro, but could be obviously utilized by intestinal microbiota, and might possess the potential to improve intestinal health.
35342881	6	14	contain	possess	1230:1236	arg1	WYP					1096:1098	WYP	1096:1098	WYP	1096:1098	The results showed that WYP was slightly digested in the gastrointestinal tract in vitro, but could be obviously utilized by intestinal microbiota, and might possess the potential to improve intestinal health.
35342881	3	15	theme	reducing	586:593	arg1	sugar					595:599	reducing sugar	586:599	reducing sugar	586:599	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	2	16	theme	WYP	402:404	arg1	characteristics					383:397	physicochemical characteristics	367:397	physicochemical characteristics of WYP	367:404	Results revealed that physicochemical characteristics of WYP were slightly altered after the simulated digestion in vitro, and its digestibility was about 8.38%.
35342881	0	17	theme	Dynamic	0:6	arg1	variations					8:17	Dynamic variations	0:17	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.	0:142	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	1	18	theme	simulated	272:280	arg1	digestion					282:290	different simulated digestion and fecal fermentation stages	262:320	digestion	282:290	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	19	from	digestion	282:290	arg1	tea					249:251	'Wuyi rock' tea	237:251	'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	237:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	19	from	digestion	282:290	arg1	WYP					254:256	WYP	254:256	WYP	254:256	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	0	20	theme	physicochemical	22:36	arg1	characteristics					38:52	physicochemical characteristics	22:52	physicochemical characteristics of oolong tea polysaccharides	22:82	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	4	21	theme	indigestible	806:817	arg1	WYP					819:821	the indigestible WYP	802:821	the indigestible WYP	802:821	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	5	22	theme	fatty	1059:1063	arg1	acids					1065:1069	short-chain fatty acids	1047:1069	short-chain fatty acids	1047:1069	Moreover, it could also enhance the generation of short-chain fatty acids.
35342881	6	23	used	utilized	1185:1192	arg2	WYP					1096:1098	WYP	1096:1098	WYP	1096:1098	The results showed that WYP was slightly digested in the gastrointestinal tract in vitro, but could be obviously utilized by intestinal microbiota, and might possess the potential to improve intestinal health.
35342881	1	24	theme	polysaccharides	216:230	arg1	characteristics					197:211	physicochemical characteristics	181:211	physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	181:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	6	25	theme	intestinal	1197:1206	arg1	microbiota					1208:1217	intestinal microbiota	1197:1217	intestinal microbiota	1197:1217	The results showed that WYP was slightly digested in the gastrointestinal tract in vitro, but could be obviously utilized by intestinal microbiota, and might possess the potential to improve intestinal health.
35342881	0	26	from	digestion	101:109	arg1	vitro					137:141	vitro	137:141	vitro	137:141	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	1	27	theme	fecal	296:300	arg1	stages					315:320	different simulated digestion and fecal fermentation stages	262:320	stages	315:320	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	3	28	theme	FT-IR	674:678	arg1	spectrum					680:687	FT-IR spectrum	674:687	FT-IR spectrum	674:687	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	1	29	theme	fermentation	302:313	arg1	stages					315:320	different simulated digestion and fecal fermentation stages	262:320	stages	315:320	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	30	theme	Wuyi	238:241	arg1	tea					249:251	'Wuyi rock' tea	237:251	'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	237:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	30	theme	Wuyi	238:241	arg1	WYP					254:256	WYP	254:256	WYP	254:256	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	0	31	theme	tea	64:66	arg1	polysaccharides					68:82	oolong tea polysaccharides	57:82	oolong tea polysaccharides	57:82	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	1	32	from	tea	249:251	arg1	characteristics					197:211	physicochemical characteristics	181:211	physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	181:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	32	from	tea	249:251	arg1	polysaccharides					216:230	polysaccharides	216:230	polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	216:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	5	33	theme	short-chain	1047:1057	arg1	acids					1065:1069	short-chain fatty acids	1047:1069	short-chain fatty acids	1047:1069	Moreover, it could also enhance the generation of short-chain fatty acids.
35342881	0	34	theme	oolong	57:62	arg1	polysaccharides					68:82	oolong tea polysaccharides	57:82	oolong tea polysaccharides	57:82	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	4	35	theme	profitable	910:919	arg1	microbes					932:939	profitable intestinal microbes	910:939	profitable intestinal microbes	910:939	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	4	35	theme	profitable	910:919	arg1	Lactococcus					963:973	Lactococcus	963:973	Lactococcus	963:973	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	4	35	theme	profitable	910:919	arg1	Bifidobacterium					980:994	Bifidobacterium	980:994	Bifidobacterium	980:994	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	4	35	theme	profitable	910:919	arg1	Bacteroides					950:960	Bacteroides	950:960	Bacteroides	950:960	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	5	36	theme	acids	1065:1069	arg1	generation					1033:1042	the generation	1029:1042	the generation of short-chain fatty acids	1029:1069	Moreover, it could also enhance the generation of short-chain fatty acids.
35342881	3	37	theme	chemical	602:609	arg1	composition					611:621	chemical composition	602:621	chemical composition	602:621	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	0	38	from	fermentation	121:132	arg1	vitro					137:141	vitro	137:141	vitro	137:141	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	1	39	theme	rock	243:246	arg1	tea					249:251	'Wuyi rock' tea	237:251	'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	237:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	39	theme	rock	243:246	arg1	WYP					254:256	WYP	254:256	WYP	254:256	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	4	40	theme	microbes	932:939	arg1	proliferation					893:905	the proliferation	889:905	the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium	889:994	Notably, the indigestible WYP could remarkably modulate the microbial composition via promoting the proliferation of profitable intestinal microbes, such as Bacteroides, Lactococcus, and Bifidobacterium.
35342881	3	41	theme	molecular	652:660	arg1	weight					662:667	molecular weight	652:667	molecular weight	652:667	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	1	42	from	characteristics	197:211	arg1	tea					249:251	'Wuyi rock' tea	237:251	'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro	237:329	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	1	42	from	characteristics	197:211	arg1	WYP					254:256	WYP	254:256	WYP	254:256	In this study, dynamic variations in physicochemical characteristics of polysaccharides from 'Wuyi rock' tea (WYP) at different simulated digestion and fecal fermentation stages in vitro were studied.
35342881	6	43	theme	gastrointestinal	1129:1144	arg1	tract					1146:1150	the gastrointestinal tract	1125:1150	the gastrointestinal tract	1125:1150	The results showed that WYP was slightly digested in the gastrointestinal tract in vitro, but could be obviously utilized by intestinal microbiota, and might possess the potential to improve intestinal health.
35342881	3	44	theme	constituent	624:634	arg1	monosaccharide					636:649	constituent monosaccharide	624:649	constituent monosaccharide	624:649	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	2	45	theme	simulated	438:446	arg1	digestion					448:456	the simulated digestion	434:456	the simulated digestion	434:456	Results revealed that physicochemical characteristics of WYP were slightly altered after the simulated digestion in vitro, and its digestibility was about 8.38%.
35342881	3	46	theme	physicochemical	519:533	arg1	composition					611:621	chemical composition	602:621	chemical composition	602:621	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	46	theme	physicochemical	519:533	arg1	weight					662:667	molecular weight	652:667	molecular weight	652:667	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	46	theme	physicochemical	519:533	arg1	characteristics					535:549	physicochemical characteristics	519:549	physicochemical characteristics	519:549	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	46	theme	physicochemical	519:533	arg1	sugar					595:599	reducing sugar	586:599	reducing sugar	586:599	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	46	theme	physicochemical	519:533	arg1	monosaccharide					636:649	constituent monosaccharide	624:649	constituent monosaccharide	624:649	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	3	46	theme	physicochemical	519:533	arg1	spectrum					680:687	FT-IR spectrum	674:687	FT-IR spectrum	674:687	Conversely, physicochemical characteristics of the indigestible WYP, including reducing sugar, chemical composition, constituent monosaccharide, molecular weight, and FT-IR spectrum, were obviously altered after the fecal fermentation in vitro, and its fermentability was about 42.18%.
35342881	0	47	theme	polysaccharides	68:82	arg1	characteristics					38:52	physicochemical characteristics	22:52	physicochemical characteristics of oolong tea polysaccharides	22:82	Dynamic variations in physicochemical characteristics of oolong tea polysaccharides during simulated digestion and fecal fermentation in vitro.
35342881	2	48	theme	physicochemical	367:381	arg1	characteristics					383:397	physicochemical characteristics	367:397	physicochemical characteristics of WYP	367:404	Results revealed that physicochemical characteristics of WYP were slightly altered after the simulated digestion in vitro, and its digestibility was about 8.38%.
35586333	7	0	theme	fibrous	998:1004	arg1	roots					1006:1010	fibrous roots	998:1010	fibrous roots	998:1010	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	1	1	theme	HPLC-FLD	204:211	arg1	method					214:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	5	2	theme	chromatogram	774:785	arg1	peaks					765:769	the peaks	761:769	the peaks of chromatogram	761:785	Ethyl acetate extraction reduced the peaks of chromatogram and improved the detection sensitivity than other agents.
35586333	8	3	from	sibiricum	1240:1248	arg1	determination					1185:1197	determination	1185:1197	determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum	1185:1273	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	0	4	with	HPLC-FLD	98:105	arg1	derivatization					123:136	pre-column derivatization	112:136	pre-column derivatization	112:136	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	3	5	theme	250	588:590	arg1	mm					592:593	mm	592:593	mm	592:593	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	6	6	theme	high	872:875	arg1	sensitivity					877:887	high sensitivity	872:887	high sensitivity	872:887	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	0	7	theme	pre-column	112:121	arg1	derivatization					123:136	pre-column derivatization	112:136	pre-column derivatization	112:136	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	0	8	from	Determination	0:12	arg1	sibiricum					60:68	Polygonatum sibiricum	48:68	Polygonatum sibiricum	48:68	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	0	8	from	Determination	0:12	arg1	odoratum					86:93	Polygonatum odoratum	74:93	Polygonatum odoratum	74:93	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	8	9	theme	monosaccharide	1202:1215	arg1	content					1217:1223	monosaccharide content	1202:1223	monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum	1202:1273	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	5	10	theme	acetate	734:740	arg1	extraction					742:751	Ethyl acetate extraction	728:751	Ethyl acetate extraction	728:751	Ethyl acetate extraction reduced the peaks of chromatogram and improved the detection sensitivity than other agents.
35586333	6	11	theme	good	910:913	arg1	relationship					922:933	good linear relationship	910:933	good linear relationship	910:933	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	9	12	from	findings	1417:1424	arg1	polysaccharides					1429:1443	polysaccharides	1429:1443	polysaccharides from Polygonatum sibiricum and Polygonatum odoratum	1429:1495	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	7	13	contain	contained	1062:1070	arg1	roots					1006:1010	fibrous roots	998:1010	fibrous roots	998:1010	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	7	13	contain	contained	1062:1070	arg2	monosaccharides					1084:1098	these seven monosaccharides	1072:1098	these seven monosaccharides	1072:1098	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	7	13	contain	contained	1062:1070	arg1	roots					988:992	roots	988:992	roots	988:992	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	3	14	theme	C18	560:562	arg1	column					580:585	C18 chromatographic column	560:585	C18 chromatographic column (250 mm × 4.6 mm, 5 μm)	560:609	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	14	theme	C18	560:562	arg1	Inertsil					544:551	Inertsil ODS-3	544:557	Inertsil ODS-3	544:557	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	0	15	from	sibiricum	60:68	arg1	Determination					0:12	Determination	0:12	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.	0:137	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	2	16	theme	labeled	469:475	arg1	compounds					492:500	fluorescently labeled monosaccharide compounds	455:500	fluorescently labeled monosaccharide compounds	455:500	The polysaccharides were de-esterified, extracted, hydrolyzed and derivatized with p-aminobenzoic acid (PABA) to obtain fluorescently labeled monosaccharide compounds, which were finally detected by HPLC-FLD.
35586333	8	17	theme	content	1217:1223	arg1	determination					1185:1197	determination	1185:1197	determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum	1185:1273	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	9	18	theme	analytical	1359:1368	arg1	tool					1370:1373	a feasible analytical tool	1348:1373	a feasible analytical tool for the study of polysaccharides	1348:1406	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	3	19	dep	column	580:585	arg1	mm					601:602	250 mm × 4.6 mm	588:602	250 mm × 4.6 mm	588:602	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	19	dep	column	580:585	arg1	μm					607:608	5 μm	605:608	5 μm	605:608	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	20	theme	chromatographic	564:578	arg1	column					580:585	C18 chromatographic column	560:585	C18 chromatographic column (250 mm × 4.6 mm, 5 μm)	560:609	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	20	theme	chromatographic	564:578	arg1	Inertsil					544:551	Inertsil ODS-3	544:557	Inertsil ODS-3	544:557	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	6	21	theme	recovery	939:946	arg1	efficiency					948:957	recovery efficiency	939:957	recovery efficiency	939:957	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	6	22	theme	strong	890:895	arg1	specificity					897:907	strong specificity	890:907	strong specificity	890:907	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	7	23	theme	highest	1109:1115	arg1	content					1132:1138	the highest monosaccharide content	1105:1138	the highest monosaccharide content	1105:1138	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	7	23	theme	highest	1109:1115	arg1	mannose					1144:1150	mannose	1144:1150	mannose	1144:1150	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	4	24	theme	emission	691:698	arg1	Em					712:713	Em	712:713	Em	712:713	The excitation wavelength (Ex) was 313 nm, and the emission wavelength (Em) was 358 nm.
35586333	4	24	theme	emission	691:698	arg1	wavelength					700:709	the emission wavelength	687:709	the emission wavelength (Em)	687:714	The excitation wavelength (Ex) was 313 nm, and the emission wavelength (Em) was 358 nm.
35586333	4	24	theme	emission	691:698	arg1	nm					724:725	358 nm	720:725	358 nm	720:725	The excitation wavelength (Ex) was 313 nm, and the emission wavelength (Em) was 358 nm.
35586333	9	25	theme	feasible	1350:1357	arg1	tool					1370:1373	a feasible analytical tool	1348:1373	a feasible analytical tool for the study of polysaccharides	1348:1406	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	7	26	theme	monosaccharide	1117:1130	arg1	content					1132:1138	the highest monosaccharide content	1105:1138	the highest monosaccharide content	1105:1138	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	7	26	theme	monosaccharide	1117:1130	arg1	mannose					1144:1150	mannose	1144:1150	mannose	1144:1150	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	9	27	theme	natural	1526:1532	arg1	industry					1543:1550	the natural medicine industry	1522:1550	the natural medicine industry	1522:1550	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	0	28	theme	composition	33:43	arg1	Determination					0:12	Determination	0:12	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.	0:137	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	9	29	theme	polysaccharides	1392:1406	arg1	study					1383:1387	the study	1379:1387	the study of polysaccharides	1379:1406	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	1	30	from	odoratum	325:332	arg1	determination					245:257	the determination	241:257	the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum	241:332	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	9	31	theme	medicine	1534:1541	arg1	industry					1543:1550	the natural medicine industry	1522:1550	the natural medicine industry	1522:1550	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	0	32	theme	polysaccharides	17:31	arg1	composition					33:43	polysaccharides composition	17:43	polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum	17:93	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	8	33	from	determination	1185:1197	arg1	sibiricum					1240:1248	Polygonatum sibiricum	1228:1248	Polygonatum sibiricum	1228:1248	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	8	33	from	determination	1185:1197	arg1	odoratum					1266:1273	Polygonatum odoratum	1254:1273	Polygonatum odoratum	1254:1273	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	3	34	theme	mm	592:593	arg1	mm					601:602	250 mm × 4.6 mm	588:602	250 mm × 4.6 mm	588:602	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	34	theme	mm	592:593	arg1	μm					607:608	5 μm	605:608	5 μm	605:608	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	35	used	used	615:618	arg2	Inertsil					544:551	Inertsil ODS-3	544:557	Inertsil ODS-3	544:557	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	35	used	used	615:618	arg2	column					580:585	C18 chromatographic column	560:585	C18 chromatographic column (250 mm × 4.6 mm, 5 μm)	560:609	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	9	36	from	odoratum	1488:1495	arg1	polysaccharides					1429:1443	polysaccharides	1429:1443	polysaccharides from Polygonatum sibiricum and Polygonatum odoratum	1429:1495	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	0	37	from	odoratum	86:93	arg1	Determination					0:12	Determination	0:12	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.	0:137	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	5	38	theme	other	831:835	arg1	agents					837:842	other agents	831:842	other agents	831:842	Ethyl acetate extraction reduced the peaks of chromatogram and improved the detection sensitivity than other agents.
35586333	2	39	theme	monosaccharide	477:490	arg1	compounds					492:500	fluorescently labeled monosaccharide compounds	455:500	fluorescently labeled monosaccharide compounds	455:500	The polysaccharides were de-esterified, extracted, hydrolyzed and derivatized with p-aminobenzoic acid (PABA) to obtain fluorescently labeled monosaccharide compounds, which were finally detected by HPLC-FLD.
35586333	5	40	theme	Ethyl	728:732	arg1	extraction					742:751	Ethyl acetate extraction	728:751	Ethyl acetate extraction	728:751	Ethyl acetate extraction reduced the peaks of chromatogram and improved the detection sensitivity than other agents.
35586333	9	41	from	sibiricum	1462:1470	arg1	polysaccharides					1429:1443	polysaccharides	1429:1443	polysaccharides from Polygonatum sibiricum and Polygonatum odoratum	1429:1495	The method established in this work provides a feasible analytical tool for the study of polysaccharides, and the findings on polysaccharides from Polygonatum sibiricum and Polygonatum odoratum can provide guidance for the natural medicine industry.
35586333	7	42	theme	odoratum	1053:1060	arg1	roots					1006:1010	fibrous roots	998:1010	fibrous roots	998:1010	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	7	42	theme	odoratum	1053:1060	arg1	roots					988:992	roots	988:992	roots	988:992	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	2	43	theme	p-aminobenzoic	418:431	arg1	PABA					439:442	PABA	439:442	PABA	439:442	The polysaccharides were de-esterified, extracted, hydrolyzed and derivatized with p-aminobenzoic acid (PABA) to obtain fluorescently labeled monosaccharide compounds, which were finally detected by HPLC-FLD.
35586333	2	43	theme	p-aminobenzoic	418:431	arg1	acid					433:436	p-aminobenzoic acid	418:436	p-aminobenzoic acid (PABA) to obtain fluorescently labeled monosaccharide compounds	418:500	The polysaccharides were de-esterified, extracted, hydrolyzed and derivatized with p-aminobenzoic acid (PABA) to obtain fluorescently labeled monosaccharide compounds, which were finally detected by HPLC-FLD.
35586333	5	44	theme	detection	804:812	arg1	sensitivity					814:824	the detection sensitivity	800:824	the detection sensitivity than other agents	800:842	Ethyl acetate extraction reduced the peaks of chromatogram and improved the detection sensitivity than other agents.
35586333	1	45	from	sibiricum	299:307	arg1	determination					245:257	the determination	241:257	the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum	241:332	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	8	46	from	odoratum	1266:1273	arg1	determination					1185:1197	determination	1185:1197	determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum	1185:1273	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	8	47	from	content	1217:1223	arg1	sibiricum					1240:1248	Polygonatum sibiricum	1228:1248	Polygonatum sibiricum	1228:1248	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	8	47	from	content	1217:1223	arg1	odoratum					1266:1273	Polygonatum odoratum	1254:1273	Polygonatum odoratum	1254:1273	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	1	48	theme	high-performance	141:156	arg1	method					214:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	1	49	from	monosaccharides	268:282	arg1	sibiricum					299:307	Polygonatum sibiricum	287:307	Polygonatum sibiricum	287:307	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	1	49	from	monosaccharides	268:282	arg1	odoratum					325:332	Polygonatum odoratum	313:332	Polygonatum odoratum	313:332	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	6	50	theme	established	849:859	arg1	method					861:866	The established method	845:866	The established method	845:866	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	1	51	from	determination	245:257	arg1	sibiricum					299:307	Polygonatum sibiricum	287:307	Polygonatum sibiricum	287:307	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	1	51	from	determination	245:257	arg1	odoratum					325:332	Polygonatum odoratum	313:332	Polygonatum odoratum	313:332	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	1	52	theme	liquid	158:163	arg1	method					214:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	1	53	theme	monosaccharides	268:282	arg1	determination					245:257	the determination	241:257	the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum	241:332	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	7	54	theme	sibiricum	1027:1035	arg1	roots					1006:1010	fibrous roots	998:1010	fibrous roots	998:1010	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	7	54	theme	sibiricum	1027:1035	arg1	roots					988:992	roots	988:992	roots	988:992	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	4	55	dep	wavelength	655:664	arg1	Ex					667:668	Ex	667:668	Ex	667:668	The excitation wavelength (Ex) was 313 nm, and the emission wavelength (Em) was 358 nm.
35586333	1	56	theme	chromatography-fluorescence	165:191	arg1	method					214:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	4	57	theme	excitation	644:653	arg1	wavelength					655:664	The excitation wavelength	640:664	The excitation wavelength (Ex)	640:669	The excitation wavelength (Ex) was 313 nm, and the emission wavelength (Em) was 358 nm.
35586333	4	57	theme	excitation	644:653	arg1	nm					679:680	313 nm	675:680	313 nm	675:680	The excitation wavelength (Ex) was 313 nm, and the emission wavelength (Em) was 358 nm.
35586333	8	58	theme	PABA-HPLC-FLD	1167:1179	arg1	sensitive					1279:1287	sensitive	1279:1287	sensitive	1279:1287	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	8	58	theme	PABA-HPLC-FLD	1167:1179	arg1	method					1157:1162	The method	1153:1162	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum	1153:1273	The method of PABA-HPLC-FLD for determination of monosaccharide content in Polygonatum sibiricum and Polygonatum odoratum was sensitive and accurate.
35586333	1	59	theme	detection	193:201	arg1	method					214:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method	139:219	A high-performance liquid chromatography-fluorescence detection (HPLC-FLD) method was established for the determination of seven monosaccharides in Polygonatum sibiricum and Polygonatum odoratum.
35586333	7	60	dep	roots	988:992	arg1	the					984:986	the	984:986	the	984:986	The results showed that the roots and fibrous roots of Polygonatum sibiricum and Polygonatum odoratum contained these seven monosaccharides, and the highest monosaccharide content was mannose.
35586333	6	61	theme	linear	915:920	arg1	relationship					922:933	good linear relationship	910:933	good linear relationship	910:933	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	6	62	contain	had	868:870	arg2	efficiency					948:957	recovery efficiency	939:957	recovery efficiency	939:957	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	6	62	contain	had	868:870	arg2	relationship					922:933	good linear relationship	910:933	good linear relationship	910:933	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	6	62	contain	had	868:870	arg1	method					861:866	The established method	845:866	The established method	845:866	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	6	62	contain	had	868:870	arg2	sensitivity					877:887	high sensitivity	872:887	high sensitivity	872:887	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	6	62	contain	had	868:870	arg2	specificity					897:907	strong specificity	890:907	strong specificity	890:907	The established method had high sensitivity, strong specificity, good linear relationship and recovery efficiency.
35586333	0	63	from	composition	33:43	arg1	sibiricum					60:68	Polygonatum sibiricum	48:68	Polygonatum sibiricum	48:68	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	0	63	from	composition	33:43	arg1	odoratum					86:93	Polygonatum odoratum	74:93	Polygonatum odoratum	74:93	Determination of polysaccharides composition in Polygonatum sibiricum and Polygonatum odoratum by HPLC-FLD with pre-column derivatization.
35586333	3	64	theme	×	595:595	arg1	mm					601:602	250 mm × 4.6 mm	588:602	250 mm × 4.6 mm	588:602	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35586333	3	64	theme	×	595:595	arg1	μm					607:608	5 μm	605:608	5 μm	605:608	Inertsil ODS-3, C18 chromatographic column (250 mm × 4.6 mm, 5 μm) was used for chromatography.
35631922	11	0	theme	structural	1755:1764	arg1	characteristics					1782:1796	The above structural and rheological characteristics	1745:1796	The above structural and rheological characteristics	1745:1796	The above structural and rheological characteristics should be taken into account when considering effective applications for this material.
35631922	11	1	theme	above	1749:1753	arg1	characteristics					1782:1796	The above structural and rheological characteristics	1745:1796	The above structural and rheological characteristics	1745:1796	The above structural and rheological characteristics should be taken into account when considering effective applications for this material.
35631922	0	2	theme	Yellow-	77:83	arg1	Cultivars					132:140	Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	69:140	Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	69:140	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	7	3	theme	FSM	1211:1213	arg1	parameters					1197:1206	the rheological parameters	1181:1206	the rheological parameters	1181:1206	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	6	4	theme	significant	929:939	arg1	variability					941:951	significant variability	929:951	significant variability in composition and rheological properties	929:993	The samples showed significant variability in composition and rheological properties depending on the cultivar and storage time.
35631922	0	5	theme	Flax	102:105	arg1	Cultivars					132:140	Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	69:140	Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	69:140	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	9	6	theme	arabinoxylan	1611:1622	arg1	content					1624:1630	the arabinoxylan content	1607:1630	the arabinoxylan content	1607:1630	In contrast, the shear-thinning and especially the dynamic viscoelastic properties depended on the arabinoxylan content.
35631922	10	7	theme	FSM	1724:1726	arg1	component					1711:1719	the elastic component	1699:1719	the elastic component of FSM in the solution	1699:1742	Proteins also affected the viscoelastic properties and maintained the elastic component of FSM in the solution.
35631922	6	8	dep	cultivar	1012:1019	arg1	the					1008:1010	the	1008:1010	the	1008:1010	The samples showed significant variability in composition and rheological properties depending on the cultivar and storage time.
35631922	5	9	theme	statistical	889:899	arg1	methods					901:907	statistical methods	889:907	statistical methods	889:907	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	9	10	theme	dynamic	1563:1569	arg1	properties					1584:1593	the dynamic viscoelastic properties	1559:1593	the dynamic viscoelastic properties	1559:1593	In contrast, the shear-thinning and especially the dynamic viscoelastic properties depended on the arabinoxylan content.
35631922	3	11	contain	contains	544:551	arg2	minerals					568:575	minerals	568:575	minerals	568:575	FSM consists mainly of two polysaccharides, namely, arabinoxylan and rhamnogalacturonan I, and it also contains some proteins, minerals, and phenolic compounds.
35631922	3	11	contain	contains	544:551	arg2	proteins					558:565	some proteins	553:565	some proteins	553:565	FSM consists mainly of two polysaccharides, namely, arabinoxylan and rhamnogalacturonan I, and it also contains some proteins, minerals, and phenolic compounds.
35631922	3	11	contain	contains	544:551	arg2	compounds					591:599	phenolic compounds	582:599	phenolic compounds	582:599	FSM consists mainly of two polysaccharides, namely, arabinoxylan and rhamnogalacturonan I, and it also contains some proteins, minerals, and phenolic compounds.
35631922	3	11	contain	contains	544:551	arg1	it					536:537	it	536:537	it	536:537	FSM consists mainly of two polysaccharides, namely, arabinoxylan and rhamnogalacturonan I, and it also contains some proteins, minerals, and phenolic compounds.
35631922	0	12	theme	Brown-Seeded	89:100	arg1	Flax					102:105	Brown-Seeded Flax	89:105	Brown-Seeded Flax	89:105	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	4	13	theme	functional	693:702	arg1	properties					704:713	functional properties	693:713	functional properties	693:713	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	7	14	theme	shear-thinning	1235:1248	arg1	behavior					1289:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior	1231:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1231:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	15	theme	proteins	1138:1145	arg1	contribution					1107:1118	the contribution	1103:1118	the contribution of less-prevalent proteins	1103:1145	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	15	theme	proteins	1138:1145	arg1	factors					1161:1167	important factors	1151:1167	important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1151:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	15	theme	proteins	1138:1145	arg1	ratio					1061:1065	the ratio	1057:1065	the ratio of two polysaccharide fractions	1057:1097	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	16	theme	viscoelastic	1276:1287	arg1	behavior					1289:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior	1231:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1231:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	4	17	theme	FSM	718:720	arg1	composition					677:687	composition	677:687	composition	677:687	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	4	17	theme	FSM	718:720	arg1	properties					704:713	functional properties	693:713	functional properties	693:713	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	7	18	theme	polysaccharide	1074:1087	arg1	fractions					1089:1097	two polysaccharide fractions	1070:1097	two polysaccharide fractions	1070:1097	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	19	theme	fractions	1089:1097	arg1	contribution					1107:1118	the contribution	1103:1118	the contribution of less-prevalent proteins	1103:1145	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	19	theme	fractions	1089:1097	arg1	factors					1161:1167	important factors	1151:1167	important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1151:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	19	theme	fractions	1089:1097	arg1	ratio					1061:1065	the ratio	1057:1065	the ratio of two polysaccharide fractions	1057:1097	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	5	20	theme	FSM	737:739	arg1	samples					741:747	FSM samples	737:747	FSM samples	737:747	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	5	21	theme	different	782:790	arg1	cultivars					792:800	different cultivars	782:800	different cultivars	782:800	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	2	22	theme	due	397:399	arg1	food					349:352	food	349:352	food	349:352	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	6	23	from	variability	941:951	arg1	composition					956:966	composition	956:966	composition	956:966	The samples showed significant variability in composition and rheological properties depending on the cultivar and storage time.
35631922	6	23	from	variability	941:951	arg1	properties					984:993	rheological properties	972:993	rheological properties	972:993	The samples showed significant variability in composition and rheological properties depending on the cultivar and storage time.
35631922	7	24	theme	rheological	1185:1195	arg1	parameters					1197:1206	the rheological parameters	1181:1206	the rheological parameters	1181:1206	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	8	25	theme	yield	1341:1345	arg1	strength					1347:1354	The yield strength	1337:1354	The yield strength	1337:1354	The yield strength and the hysteresis loop were found to be associated with the contribution of the pectin fraction, which included homogalacturonan and rhamnogalacturonan I.
35631922	5	26	theme	cultivars	792:800	arg1	seeds					773:777	flax seeds	768:777	flax seeds of different cultivars and harvest years	768:818	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	0	27	theme	Chemical	0:7	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	0	28	dep	Yellow-	77:83	arg1	L.					128:129	Linum usitatissimum L.	108:129	Linum usitatissimum L.	108:129	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	0	29	theme	Rheological	25:35	arg1	Properties					37:46	Rheological Properties	25:46	Rheological Properties	25:46	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	10	30	from	component	1711:1719	arg1	solution					1735:1742	the solution	1731:1742	the solution	1731:1742	Proteins also affected the viscoelastic properties and maintained the elastic component of FSM in the solution.
35631922	9	31	theme	viscoelastic	1571:1582	arg1	properties					1584:1593	the dynamic viscoelastic properties	1559:1593	the dynamic viscoelastic properties	1559:1593	In contrast, the shear-thinning and especially the dynamic viscoelastic properties depended on the arabinoxylan content.
35631922	7	32	theme	dynamic	1268:1274	arg1	behavior					1289:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior	1231:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1231:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	5	33	theme	structural	831:840	arg1	properties					858:867	their structural and rheological properties	825:867	their structural and rheological properties	825:867	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	3	34	theme	phenolic	582:589	arg1	compounds					591:599	phenolic compounds	582:599	phenolic compounds	582:599	FSM consists mainly of two polysaccharides, namely, arabinoxylan and rhamnogalacturonan I, and it also contains some proteins, minerals, and phenolic compounds.
35631922	2	35	theme	Flaxseed	270:277	arg1	mucilage					279:286	Flaxseed mucilage	270:286	Flaxseed mucilage (FSM)	270:292	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	2	35	theme	Flaxseed	270:277	arg1	example					300:306	an example	297:306	an example of such a material	297:325	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	2	35	theme	Flaxseed	270:277	arg1	FSM					289:291	FSM	289:291	FSM	289:291	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	5	36	theme	harvest	806:812	arg1	years					814:818	harvest years	806:818	harvest years	806:818	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	2	37	theme	rheological	417:427	arg1	properties					429:438	its suitable rheological properties	404:438	its suitable rheological properties	404:438	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	4	38	dep	composition	677:687	arg1	the					673:675	the	673:675	the	673:675	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	0	39	theme	Cultivars	132:140	arg1	Mucilages					56:64	Seed Mucilages	51:64	Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	51:140	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	7	40	theme	less-prevalent	1123:1136	arg1	proteins					1138:1145	less-prevalent proteins	1123:1145	less-prevalent proteins	1123:1145	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	8	41	theme	hysteresis	1364:1373	arg1	loop					1375:1378	the hysteresis loop	1360:1378	the hysteresis loop	1360:1378	The yield strength and the hysteresis loop were found to be associated with the contribution of the pectin fraction, which included homogalacturonan and rhamnogalacturonan I.
35631922	5	42	theme	flax	768:771	arg1	seeds					773:777	flax seeds	768:777	flax seeds of different cultivars and harvest years	768:818	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	2	43	theme	suitable	408:415	arg1	properties					429:438	its suitable rheological properties	404:438	its suitable rheological properties	404:438	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	11	44	theme	effective	1844:1852	arg1	applications					1854:1865	effective applications	1844:1865	effective applications for this material	1844:1883	The above structural and rheological characteristics should be taken into account when considering effective applications for this material.
35631922	2	45	theme	pharmaceutical	369:382	arg1	applications					384:395	pharmaceutical applications	369:395	pharmaceutical applications	369:395	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	10	46	theme	viscoelastic	1660:1671	arg1	properties					1673:1682	the viscoelastic properties	1656:1682	the viscoelastic properties	1656:1682	Proteins also affected the viscoelastic properties and maintained the elastic component of FSM in the solution.
35631922	0	47	theme	Mucilages	56:64	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition	0:19	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	0	47	theme	Mucilages	56:64	arg1	Properties					37:46	Rheological Properties	25:46	Rheological Properties	25:46	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	7	48	theme	important	1151:1159	arg1	contribution					1107:1118	the contribution	1103:1118	the contribution of less-prevalent proteins	1103:1145	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	48	theme	important	1151:1159	arg1	factors					1161:1167	important factors	1151:1167	important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1151:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	7	48	theme	important	1151:1159	arg1	ratio					1061:1065	the ratio	1057:1065	the ratio of two polysaccharide fractions	1057:1097	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	4	49	theme	harvest	640:646	arg1	genotype					606:613	genotype	606:613	genotype	606:613	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	4	49	theme	harvest	640:646	arg1	year					623:626	the year	619:626	the year	619:626	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	0	50	theme	Seed	51:54	arg1	Mucilages					56:64	Seed Mucilages	51:64	Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	51:140	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	11	51	theme	rheological	1770:1780	arg1	characteristics					1782:1796	The above structural and rheological characteristics	1745:1796	The above structural and rheological characteristics	1745:1796	The above structural and rheological characteristics should be taken into account when considering effective applications for this material.
35631922	8	52	theme	pectin	1437:1442	arg1	fraction					1444:1451	the pectin fraction	1433:1451	the pectin fraction	1433:1451	The yield strength and the hysteresis loop were found to be associated with the contribution of the pectin fraction, which included homogalacturonan and rhamnogalacturonan I.
35631922	7	53	theme	material	1306:1313	arg1	behavior					1289:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior	1231:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1231:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	4	54	theme	flax	635:638	arg1	harvest					640:646	the flax harvest	631:646	the flax harvest	631:646	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	7	55	theme	aqueous	1318:1324	arg1	solutions					1326:1334	aqueous solutions	1318:1334	aqueous solutions	1318:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	5	56	attach	isolated	754:761	arg1	seeds					773:777	flax seeds	768:777	flax seeds of different cultivars and harvest years	768:818	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	5	56	attach	isolated	754:761	arg2	samples					741:747	FSM samples	737:747	FSM samples	737:747	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	10	57	theme	elastic	1703:1709	arg1	component					1711:1719	the elastic component	1699:1719	the elastic component of FSM in the solution	1699:1742	Proteins also affected the viscoelastic properties and maintained the elastic component of FSM in the solution.
35631922	6	58	theme	rheological	972:982	arg1	properties					984:993	rheological properties	972:993	rheological properties	972:993	The samples showed significant variability in composition and rheological properties depending on the cultivar and storage time.
35631922	4	59	dep	genotype	606:613	arg1	The					602:604	The	602:604	The	602:604	The genotype and the year of the flax harvest can significantly affect the composition and functional properties of FSM.
35631922	7	60	from	behavior	1289:1296	arg1	solutions					1326:1334	aqueous solutions	1318:1334	aqueous solutions	1318:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	5	61	theme	rheological	846:856	arg1	properties					858:867	their structural and rheological properties	825:867	their structural and rheological properties	825:867	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
35631922	1	62	theme	viscous	203:209	arg1	matter					211:216	viscous matter	203:216	viscous matter that can retain water and thus support germination	203:267	When seeds sown in the soil become wet, their hulls secrete viscous matter that can retain water and thus support germination.
35631922	0	63	theme	Various	69:75	arg1	Cultivars					132:140	Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	69:140	Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars	69:140	Chemical Composition and Rheological Properties of Seed Mucilages of Various Yellow- and Brown-Seeded Flax (Linum usitatissimum L.) Cultivars.
35631922	8	64	theme	fraction	1444:1451	arg1	contribution					1417:1428	the contribution	1413:1428	the contribution of the pectin fraction, which included homogalacturonan and rhamnogalacturonan I	1413:1509	The yield strength and the hysteresis loop were found to be associated with the contribution of the pectin fraction, which included homogalacturonan and rhamnogalacturonan I.
35631922	2	65	theme	material	318:325	arg1	mucilage					279:286	Flaxseed mucilage	270:286	Flaxseed mucilage (FSM)	270:292	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	2	65	theme	material	318:325	arg1	example					300:306	an example	297:306	an example of such a material	297:325	Flaxseed mucilage (FSM) is an example of such a material and is attractive for food, cosmetic, and pharmaceutical applications due to its suitable rheological properties.
35631922	6	66	theme	storage	1025:1031	arg1	time					1033:1036	storage time	1025:1036	storage time	1025:1036	The samples showed significant variability in composition and rheological properties depending on the cultivar and storage time.
35631922	7	67	theme	thixotropic	1251:1261	arg1	behavior					1289:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior	1231:1296	the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions	1231:1334	It was found that the ratio of two polysaccharide fractions and the contribution of less-prevalent proteins are important factors determining the rheological parameters of FSM, characterizing the shear-thinning, thixotropic, and dynamic viscoelastic behavior of this material in aqueous solutions.
35631922	5	68	theme	years	814:818	arg1	seeds					773:777	flax seeds	768:777	flax seeds of different cultivars and harvest years	768:818	In this work, FSM samples were isolated from flax seeds of different cultivars and harvest years, and their structural and rheological properties were compared using statistical methods.
36824734	10	0	theme	elicited	1532:1539	arg1	depletion					1550:1558	effector-cell elicited platelet depletion	1518:1558	effector-cell elicited platelet depletion	1518:1558	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	2	1	theme	distinct	325:332	arg1	responses					343:351	distinct effector responses	325:351	distinct effector responses	325:351	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	3	2	theme	Fc	552:553	arg1	subclasses					555:564	certain Fc subclasses	544:564	certain Fc subclasses	544:564	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	9	3	theme	refined	1419:1425	arg1	estimates					1427:1435	refined estimates	1419:1435	refined estimates of these affinities	1419:1455	We found that the affinities of these interactions are different than previously reported, and that the binding model could be used to provide refined estimates of these affinities.
36824734	8	4	theme	low-affinity	1227:1238	arg1	interactions					1240:1251	several low-affinity interactions	1219:1251	several low-affinity interactions mostly involving IgG2	1219:1273	Binding quantitatively matched a mechanistic binding model, except for several low-affinity interactions mostly involving IgG2.
36824734	10	5	theme	humanized	1563:1571	arg1	mice					1573:1576	humanized mice	1563:1576	humanized mice	1563:1576	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	11	6	theme	previous	1660:1667	arg1	view					1669:1672	the previous view in which IgG2 poorly engages with effector populations	1656:1727	the previous view in which IgG2 poorly engages with effector populations	1656:1727	Contrary to the previous view in which IgG2 poorly engages with effector populations, we observe appreciable binding through avidity, but insufficient amounts to observe immune effector responses.
36824734	13	7	theme	cell	2408:2411	arg1	clearance					2413:2421	effector-elicited cell clearance	2390:2421	effector-elicited cell clearance	2390:2421	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	8	theme	low-affinity	2258:2269	arg1	interactions					2288:2299	low-affinity IgG-Fcγ receptor interactions	2258:2299	low-affinity IgG-Fcγ receptor interactions	2258:2299	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	7	9	theme	complexes	1068:1076	arg1	properties					1025:1034	the binding properties	1013:1034	the binding properties of the mixed-composition immune complexes	1013:1076	We found that the binding properties of the mixed-composition immune complexes fell along a continuum between those of the corresponding pure cases.
36824734	9	10	theme	affinities	1446:1455	arg1	estimates					1427:1435	refined estimates	1419:1435	refined estimates of these affinities	1419:1455	We found that the affinities of these interactions are different than previously reported, and that the binding model could be used to provide refined estimates of these affinities.
36824734	8	11	theme	binding	1193:1199	arg1	model					1201:1205	a mechanistic binding model	1179:1205	a mechanistic binding model	1179:1205	Binding quantitatively matched a mechanistic binding model, except for several low-affinity interactions mostly involving IgG2.
36824734	7	12	theme	mixed-composition	1043:1059	arg1	complexes					1068:1076	the mixed-composition immune complexes	1039:1076	the mixed-composition immune complexes	1039:1076	We found that the binding properties of the mixed-composition immune complexes fell along a continuum between those of the corresponding pure cases.
36824734	2	13	theme	Fc	285:286	arg1	domain					288:293	The Fc domain	281:293	The Fc domain of IgG	281:300	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	2	13	theme	Fc	285:286	arg1	IgG					298:300	IgG	298:300	IgG	298:300	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	1	14	theme	target	193:198	arg1	antigens					200:207	target antigens	193:207	target antigens	193:207	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36824734	12	15	theme	mixed	1967:1971	arg1	composition					1976:1986	mixed Fc composition	1967:1986	mixed Fc composition	1967:1986	Overall, this work demonstrates a quantitative framework for reasoning about effector response regulation arising from IgG of mixed Fc composition.
36824734	6	16	theme	receptor	930:937	arg1	binding					939:945	Fcγ receptor binding	926:945	Fcγ receptor binding to immune complexes of mixed Fc domain composition	926:996	Here, we measured Fcγ receptor binding to immune complexes of mixed Fc domain composition.
36824734	10	17	theme	cell	1626:1629	arg1	populations					1631:1641	the relevant effector cell populations	1604:1641	the relevant effector cell populations	1604:1641	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	13	18	theme	binding	2152:2158	arg1	model					2160:2164	multivalent binding model	2140:2164	multivalent binding model	2140:2164	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	12	19	theme	composition	1976:1986	arg1	IgG					1960:1962	IgG	1960:1962	IgG of mixed Fc composition	1960:1986	Overall, this work demonstrates a quantitative framework for reasoning about effector response regulation arising from IgG of mixed Fc composition.
36824734	10	20	theme	relevant	1608:1615	arg1	populations					1631:1641	the relevant effector cell populations	1604:1641	the relevant effector cell populations	1604:1641	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	11	21	theme	effector	1821:1828	arg1	responses					1830:1838	immune effector responses	1814:1838	immune effector responses	1814:1838	Contrary to the previous view in which IgG2 poorly engages with effector populations, we observe appreciable binding through avidity, but insufficient amounts to observe immune effector responses.
36824734	7	22	theme	binding	1017:1023	arg1	properties					1025:1034	the binding properties	1013:1034	the binding properties of the mixed-composition immune complexes	1013:1076	We found that the binding properties of the mixed-composition immune complexes fell along a continuum between those of the corresponding pure cases.
36824734	13	23	theme	constituent	2100:2110	arg1	equilibrium					2127:2137	each constituent IgG species.An equilibrium	2095:2137	each constituent IgG species.An equilibrium	2095:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	3	24	theme	distinct	588:595	arg1	responses					604:612	distinct immune responses	588:612	distinct immune responses	588:612	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	13	25	dep	points	1997:2002	arg1	blend					2059:2063	a blend	2057:2063	a blend of the binding properties for each constituent IgG species.An equilibrium	2057:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	25	dep	points	1997:2002	arg1	behavior					2016:2023	The binding behavior	2004:2023	The binding behavior of mixed Fc immune complexes	2004:2052	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	4	26	theme	antigen-IgG	709:719	arg1	complexes					728:736	antigen-IgG immune complexes	709:736	antigen-IgG immune complexes	709:736	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	13	27	theme	species.An	2116:2125	arg1	equilibrium					2127:2137	each constituent IgG species.An equilibrium	2095:2137	each constituent IgG species.An equilibrium	2095:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	1	28	theme	G	117:117	arg1	antibodies					119:128	Immunoglobulin (Ig)G antibodies	98:128	Immunoglobulin (Ig)G antibodies	98:128	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36824734	13	29	theme	binding	2072:2078	arg1	properties					2080:2089	the binding properties	2068:2089	the binding properties for each constituent IgG species.An equilibrium	2068:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	30	theme	immune	2302:2307	arg1	complexes					2309:2317	immune complexes	2302:2317	immune complexes	2302:2317	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	6	31	theme	domain	979:984	arg1	composition					986:996	mixed Fc domain composition	970:996	mixed Fc domain composition	970:996	Here, we measured Fcγ receptor binding to immune complexes of mixed Fc domain composition.
36824734	4	32	theme	domain	687:692	arg1	properties					694:703	Fc domain properties	684:703	Fc domain properties	684:703	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	1	33	theme	effector	148:155	arg1	responses					157:165	immune effector responses	141:165	immune effector responses	141:165	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36824734	6	34	theme	mixed	970:974	arg1	composition					986:996	mixed Fc domain composition	970:996	mixed Fc domain composition	970:996	Here, we measured Fcγ receptor binding to immune complexes of mixed Fc domain composition.
36824734	0	35	theme	affinity	78:85	arg1	estimates					87:95	FcR affinity estimates	74:95	FcR affinity estimates	74:95	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	2	36	theme	cell	385:388	arg1	types					390:394	several different immune cell types	360:394	several different immune cell types	360:394	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	9	37	theme	interactions	1314:1325	arg1	different					1331:1339	different	1331:1339	different	1331:1339	We found that the affinities of these interactions are different than previously reported, and that the binding model could be used to provide refined estimates of these affinities.
36824734	9	37	theme	interactions	1314:1325	arg1	affinities					1294:1303	the affinities	1290:1303	the affinities of these interactions	1290:1325	We found that the affinities of these interactions are different than previously reported, and that the binding model could be used to provide refined estimates of these affinities.
36824734	2	38	theme	Fc	436:437	arg1	glycosylation					446:458	Fc domain glycosylation	436:458	Fc domain glycosylation	436:458	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	2	39	theme	different	368:376	arg1	types					390:394	several different immune cell types	360:394	several different immune cell types	360:394	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	0	40	theme	Mixed	0:4	arg1	complexes					20:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	1	41	theme	Fcγ	266:268	arg1	receptors					270:278	the Fcγ receptors	262:278	the Fcγ receptors	262:278	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36824734	12	42	theme	effector	1918:1925	arg1	regulation					1936:1945	effector response regulation	1918:1945	effector response regulation arising from IgG of mixed Fc composition	1918:1986	Overall, this work demonstrates a quantitative framework for reasoning about effector response regulation arising from IgG of mixed Fc composition.
36824734	10	43	theme	binding	1492:1498	arg1	model					1500:1504	the binding model	1488:1504	the binding model	1488:1504	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	0	44	theme	Fc	10:11	arg1	complexes					20:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	13	45	theme	receptor	2279:2286	arg1	interactions					2288:2299	low-affinity IgG-Fcγ receptor interactions	2258:2299	low-affinity IgG-Fcγ receptor interactions	2258:2299	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	1	46	theme	Immunoglobulin	98:111	arg1	antibodies					119:128	Immunoglobulin (Ig)G antibodies	98:128	Immunoglobulin (Ig)G antibodies	98:128	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36824734	4	47	theme	Fc	799:800	arg1	forms					802:806	Fc forms	799:806	Fc forms	799:806	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	7	48	theme	pure	1136:1139	arg1	cases					1141:1145	the corresponding pure cases	1118:1145	the corresponding pure cases	1118:1145	We found that the binding properties of the mixed-composition immune complexes fell along a continuum between those of the corresponding pure cases.
36824734	13	49	theme	mixed	2220:2224	arg1	composition.Particularly					2229:2252	mixed Fc composition.Particularly	2220:2252	mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions	2220:2299	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	10	50	theme	effector-cell	1518:1530	arg1	depletion					1550:1558	effector-cell elicited platelet depletion	1518:1558	effector-cell elicited platelet depletion	1518:1558	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	13	51	theme	immune	2037:2042	arg1	complexes					2044:2052	mixed Fc immune complexes	2028:2052	mixed Fc immune complexes	2028:2052	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	0	52	theme	blended	38:44	arg1	profiles					54:61	blended binding profiles	38:61	blended binding profiles	38:61	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	13	53	theme	composition.Particularly	2229:2252	arg1	complexes					2207:2215	immune complexes	2200:2215	immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions	2200:2299	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	9	54	theme	binding	1380:1386	arg1	model					1388:1392	the binding model	1376:1392	the binding model	1376:1392	We found that the affinities of these interactions are different than previously reported, and that the binding model could be used to provide refined estimates of these affinities.
36824734	5	55	dep	Whether	809:815	arg1	influences					848:857	influences	848:857	influences immune effector responses	848:883	Whether and how this mixed composition influences immune effector responses has not been examined.
36824734	10	56	theme	platelet	1541:1548	arg1	depletion					1550:1558	effector-cell elicited platelet depletion	1518:1558	effector-cell elicited platelet depletion	1518:1558	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	13	57	theme	mixed	2028:2032	arg1	complexes					2044:2052	mixed Fc immune complexes	2028:2052	mixed Fc immune complexes	2028:2052	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	2	58	theme	effector	334:341	arg1	responses					343:351	distinct effector responses	325:351	distinct effector responses	325:351	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	1	59	theme	effector	243:250	arg1	cells					252:256	various effector cells	235:256	various effector cells	235:256	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36824734	9	60	used	used	1403:1406	arg2	model					1388:1392	the binding model	1376:1392	the binding model	1376:1392	We found that the affinities of these interactions are different than previously reported, and that the binding model could be used to provide refined estimates of these affinities.
36824734	13	61	theme	Summary	1989:1995	arg1	points					1997:2002	Summary points	1989:2002	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.	1989:2440	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	3	62	theme	certain	544:550	arg1	subclasses					555:564	certain Fc subclasses	544:564	certain Fc subclasses	544:564	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	3	63	theme	glycans	569:575	arg1	inclusion					531:539	the inclusion	527:539	the inclusion of certain Fc subclasses or glycans	527:575	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	5	64	theme	mixed	830:834	arg1	composition					836:846	this mixed composition	825:846	this mixed composition	825:846	Whether and how this mixed composition influences immune effector responses has not been examined.
36824734	8	65	theme	several	1219:1225	arg1	interactions					1240:1251	several low-affinity interactions	1219:1251	several low-affinity interactions mostly involving IgG2	1219:1273	Binding quantitatively matched a mechanistic binding model, except for several low-affinity interactions mostly involving IgG2.
36824734	13	66	theme	effector-elicited	2390:2406	arg1	clearance					2413:2421	effector-elicited cell clearance	2390:2421	effector-elicited cell clearance	2390:2421	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	7	67	theme	immune	1061:1066	arg1	complexes					1068:1076	the mixed-composition immune complexes	1039:1076	the mixed-composition immune complexes	1039:1076	We found that the binding properties of the mixed-composition immune complexes fell along a continuum between those of the corresponding pure cases.
36824734	2	68	theme	IgG	298:300	arg1	domain					288:293	The Fc domain	281:293	The Fc domain of IgG	281:300	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	2	68	theme	IgG	298:300	arg1	IgG					298:300	IgG	298:300	IgG	298:300	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	6	69	theme	immune	950:955	arg1	complexes					957:965	immune complexes	950:965	immune complexes of mixed Fc domain composition	950:996	Here, we measured Fcγ receptor binding to immune complexes of mixed Fc domain composition.
36824734	11	70	theme	immune	1814:1819	arg1	responses					1830:1838	immune effector responses	1814:1838	immune effector responses	1814:1838	Contrary to the previous view in which IgG2 poorly engages with effector populations, we observe appreciable binding through avidity, but insufficient amounts to observe immune effector responses.
36824734	3	71	theme	subclasses	555:564	arg1	inclusion					531:539	the inclusion	527:539	the inclusion of certain Fc subclasses or glycans	527:575	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	13	72	theme	FcγR	2362:2365	arg1	estimates					2334:2342	better estimates	2327:2342	better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice	2327:2439	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	8	73	theme	mechanistic	1181:1191	arg1	model					1201:1205	a mechanistic binding model	1179:1205	a mechanistic binding model	1179:1205	Binding quantitatively matched a mechanistic binding model, except for several low-affinity interactions mostly involving IgG2.
36824734	13	74	theme	multivalent	2140:2150	arg1	model					2160:2164	multivalent binding model	2140:2164	multivalent binding model	2140:2164	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	3	75	theme	immune	597:602	arg1	responses					604:612	distinct immune responses	588:612	distinct immune responses	588:612	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	5	76	theme	immune	859:864	arg1	responses					875:883	immune effector responses	859:883	immune effector responses	859:883	Whether and how this mixed composition influences immune effector responses has not been examined.
36824734	6	77	theme	Fcγ	926:928	arg1	binding					939:945	Fcγ receptor binding	926:945	Fcγ receptor binding to immune complexes of mixed Fc domain composition	926:996	Here, we measured Fcγ receptor binding to immune complexes of mixed Fc domain composition.
36824734	10	78	theme	effector	1617:1624	arg1	populations					1631:1641	the relevant effector cell populations	1604:1641	the relevant effector cell populations	1604:1641	Finally, we demonstrated that the binding model can predict effector-cell elicited platelet depletion in humanized mice, with the model inferring the relevant effector cell populations.
36824734	13	79	theme	better	2327:2332	arg1	estimates					2334:2342	better estimates	2327:2342	better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice	2327:2439	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	12	80	theme	Fc	1973:1974	arg1	composition					1976:1986	mixed Fc composition	1967:1986	mixed Fc composition	1967:1986	Overall, this work demonstrates a quantitative framework for reasoning about effector response regulation arising from IgG of mixed Fc composition.
36824734	4	81	theme	properties	694:703	arg1	mixtures					672:679	mixtures	672:679	mixtures of Fc domain properties	672:703	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	13	82	theme	IgG	2112:2114	arg1	equilibrium					2127:2137	each constituent IgG species.An equilibrium	2095:2137	each constituent IgG species.An equilibrium	2095:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	5	83	theme	effector	866:873	arg1	responses					875:883	immune effector responses	859:883	immune effector responses	859:883	Whether and how this mixed composition influences immune effector responses has not been examined.
36824734	6	84	theme	composition	986:996	arg1	complexes					957:965	immune complexes	950:965	immune complexes of mixed Fc domain composition	950:996	Here, we measured Fcγ receptor binding to immune complexes of mixed Fc domain composition.
36824734	11	85	theme	appreciable	1741:1751	arg1	binding					1753:1759	appreciable binding	1741:1759	appreciable binding	1741:1759	Contrary to the previous view in which IgG2 poorly engages with effector populations, we observe appreciable binding through avidity, but insufficient amounts to observe immune effector responses.
36824734	0	86	theme	FcR	74:76	arg1	estimates					87:95	FcR affinity estimates	74:95	FcR affinity estimates	74:95	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	13	87	theme	properties	2080:2089	arg1	blend					2059:2063	a blend	2057:2063	a blend of the binding properties for each constituent IgG species.An equilibrium	2057:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	87	theme	properties	2080:2089	arg1	behavior					2016:2023	The binding behavior	2004:2023	The binding behavior of mixed Fc immune complexes	2004:2052	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	1	88	theme	immune	141:146	arg1	responses					157:165	immune effector responses	141:165	immune effector responses	141:165	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36824734	6	89	theme	Fc	976:977	arg1	composition					986:996	mixed Fc domain composition	970:996	mixed Fc domain composition	970:996	Here, we measured Fcγ receptor binding to immune complexes of mixed Fc domain composition.
36824734	4	90	theme	Fc	684:685	arg1	properties					694:703	Fc domain properties	684:703	Fc domain properties	684:703	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	11	91	theme	insufficient	1782:1793	arg1	amounts					1795:1801	insufficient amounts	1782:1801	insufficient amounts	1782:1801	Contrary to the previous view in which IgG2 poorly engages with effector populations, we observe appreciable binding through avidity, but insufficient amounts to observe immune effector responses.
36824734	13	92	theme	binding	2367:2373	arg1	model					2375:2379	binding model	2367:2379	binding model	2367:2379	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	93	dep	estimates	2334:2342	arg1	predicts					2381:2388	predicts	2381:2388	predicts effector-elicited cell clearance in humanized mice	2381:2439	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	2	94	theme	immune	378:383	arg1	types					390:394	several different immune cell types	360:394	several different immune cell types	360:394	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	0	95	theme	IgG	6:8	arg1	complexes					20:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	12	96	theme	response	1927:1934	arg1	regulation					1936:1945	effector response regulation	1918:1945	effector response regulation arising from IgG of mixed Fc composition	1918:1986	Overall, this work demonstrates a quantitative framework for reasoning about effector response regulation arising from IgG of mixed Fc composition.
36824734	4	97	theme	immune	721:726	arg1	complexes					728:736	antigen-IgG immune complexes	709:736	antigen-IgG immune complexes	709:736	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	2	98	theme	several	360:366	arg1	types					390:394	several different immune cell types	360:394	several different immune cell types	360:394	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	0	99	theme	immune	13:18	arg1	complexes					20:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes	0:28	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	13	100	theme	IgG-Fcγ	2271:2277	arg1	interactions					2288:2299	low-affinity IgG-Fcγ receptor interactions	2258:2299	low-affinity IgG-Fcγ receptor interactions	2258:2299	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	12	101	theme	quantitative	1875:1886	arg1	framework					1888:1896	a quantitative framework	1873:1896	a quantitative framework for reasoning	1873:1910	Overall, this work demonstrates a quantitative framework for reasoning about effector response regulation arising from IgG of mixed Fc composition.
36824734	3	102	theme	Extensive	461:469	arg1	characterization					471:486	Extensive characterization	461:486	Extensive characterization of these interactions	461:508	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	7	103	theme	corresponding	1122:1134	arg1	cases					1141:1145	the corresponding pure cases	1118:1145	the corresponding pure cases	1118:1145	We found that the binding properties of the mixed-composition immune complexes fell along a continuum between those of the corresponding pure cases.
36824734	13	104	theme	Fc	2034:2035	arg1	complexes					2044:2052	mixed Fc immune complexes	2028:2052	mixed Fc immune complexes	2028:2052	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	0	105	theme	binding	46:52	arg1	profiles					54:61	blended binding profiles	38:61	blended binding profiles	38:61	Mixed IgG Fc immune complexes exhibit blended binding profiles and refine FcR affinity estimates.
36824734	13	106	theme	Fc	2226:2227	arg1	composition.Particularly					2229:2252	mixed Fc composition.Particularly	2220:2252	mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions	2220:2299	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	2	107	theme	domain	439:444	arg1	glycosylation					446:458	Fc domain glycosylation	436:458	Fc domain glycosylation	436:458	The Fc domain of IgG can promote or inhibit distinct effector responses across several different immune cell types through variation based on subclass and Fc domain glycosylation.
36824734	4	108	theme	forms	802:806	arg1	combination					784:794	a combination	782:794	a combination of Fc forms	782:806	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	13	109	theme	complexes	2044:2052	arg1	behavior					2016:2023	The binding behavior	2004:2023	The binding behavior of mixed Fc immune complexes	2004:2052	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	109	theme	complexes	2044:2052	arg1	blend					2059:2063	a blend	2057:2063	a blend of the binding properties for each constituent IgG species.An equilibrium	2057:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	3	110	theme	interactions	497:508	arg1	characterization					471:486	Extensive characterization	461:486	Extensive characterization of these interactions	461:508	Extensive characterization of these interactions has revealed how the inclusion of certain Fc subclasses or glycans results in distinct immune responses.
36824734	13	111	theme	humanized	2426:2434	arg1	mice					2436:2439	humanized mice	2426:2439	humanized mice	2426:2439	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	11	112	theme	effector	1708:1715	arg1	populations					1717:1727	effector populations	1708:1727	effector populations	1708:1727	Contrary to the previous view in which IgG2 poorly engages with effector populations, we observe appreciable binding through avidity, but insufficient amounts to observe immune effector responses.
36824734	13	113	theme	binding	2008:2014	arg1	behavior					2016:2023	The binding behavior	2004:2023	The binding behavior of mixed Fc immune complexes	2004:2052	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	113	theme	binding	2008:2014	arg1	blend					2059:2063	a blend	2057:2063	a blend of the binding properties for each constituent IgG species.An equilibrium	2057:2137	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	13	114	theme	immune	2200:2205	arg1	complexes					2207:2215	immune complexes	2200:2215	immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions	2200:2299	Summary points The binding behavior of mixed Fc immune complexes is a blend of the binding properties for each constituent IgG species.An equilibrium, multivalent binding model can be generalized to incorporate immune complexes of mixed Fc composition.Particularly for low-affinity IgG-Fcγ receptor interactions, immune complexes provide better estimates of affinities.The FcγR binding model predicts effector-elicited cell clearance in humanized mice.
36824734	4	115	theme	immune	625:630	arg1	response					632:639	an immune response	622:639	an immune response	622:639	During an immune response, however, IgG is produced with mixtures of Fc domain properties, so antigen-IgG immune complexes are likely to almost always be comprised of a combination of Fc forms.
36824734	1	116	theme	various	235:241	arg1	cells					252:256	various effector cells	235:256	various effector cells	235:256	Immunoglobulin (Ig)G antibodies coordinate immune effector responses by selectively binding to target antigens and then interacting with various effector cells via the Fcγ receptors.
36214541	4	0	theme	structure-activity	356:373	arg1	relationship					375:386	the structure-activity relationship	352:386	the structure-activity relationship of DOP	352:393	However, studies on the structure-activity relationship of DOP are rarely reported.
36214541	0	1	theme	catenatum	111:119	arg1	Lindl					121:125	Dendrobium catenatum Lindl	100:125	Dendrobium catenatum Lindl	100:125	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	0	2	from	characterisation	11:26	arg1	Lindl					121:125	Dendrobium catenatum Lindl	100:125	Dendrobium catenatum Lindl	100:125	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	7	3	theme	-D-Manp	692:698	arg1	linkage					674:680	the linkage	670:680	the linkage of β-(1→4)-D-Manp	670:698	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	7	3	theme	-D-Manp	692:698	arg1	structures					646:655	The main structures	637:655	The main structures of DOPs	637:663	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	7	4	theme	2-O-	720:723	arg1	groups					743:748	some attached 2-O- or 3-O-acetylated groups	706:748	some attached 2-O- or 3-O-acetylated groups	706:748	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	8	5	theme	antioxidant	884:894	arg1	related					921:927	related	921:927	related	921:927	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	5	theme	antioxidant	884:894	arg1	activity					896:903	the higher antioxidant activity	873:903	the higher antioxidant activity	873:903	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	9	6	from	application	1098:1108	arg1	industries					1149:1158	the pharmaceutical and food industries	1121:1158	the pharmaceutical and food industries	1121:1158	Our results provide a more accurate basis for the application of DOPs in the pharmaceutical and food industries.
36214541	8	7	theme	lower	990:994	arg1	degree					996:1001	lower degree	990:1001	lower degree of acetylation	990:1016	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	8	theme	higher	877:882	arg1	related					921:927	related	921:927	related	921:927	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	8	theme	higher	877:882	arg1	activity					896:903	the higher antioxidant activity	873:903	the higher antioxidant activity	873:903	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	9	9	theme	pharmaceutical	1125:1138	arg1	industries					1149:1158	the pharmaceutical and food industries	1121:1158	the pharmaceutical and food industries	1121:1158	Our results provide a more accurate basis for the application of DOPs in the pharmaceutical and food industries.
36214541	8	10	theme	acetylation	1006:1016	arg1	weight					952:957	its lower molecular weight	932:957	its lower molecular weight	932:957	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	10	theme	acetylation	1006:1016	arg1	content					1039:1045	higher phenolic content	1023:1045	higher phenolic content	1023:1045	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	10	theme	acetylation	1006:1016	arg1	proportion					975:984	higher mannose proportion	960:984	higher mannose proportion	960:984	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	10	theme	acetylation	1006:1016	arg1	degree					996:1001	lower degree	990:1001	lower degree of acetylation	990:1016	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	7	11	theme	DOPs	660:663	arg1	linkage					674:680	the linkage	670:680	the linkage of β-(1→4)-D-Manp	670:698	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	7	11	theme	DOPs	660:663	arg1	structures					646:655	The main structures	637:655	The main structures of DOPs	637:663	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	5	12	theme	composition	525:535	arg1	ratio					501:505	the ratio	497:505	the ratio of monosaccharide composition and molecular weight	497:556	Here, two polysaccharides named DOP-1 and DOP-2 were obtained, which differed in the ratio of monosaccharide composition and molecular weight.
36214541	8	13	theme	DOP-1	829:833	arg1	assays					819:824	the DPPH, hydroxyl and superoxide radicals scavenging assays	765:824	the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2	765:843	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	6	14	theme	Structural	559:568	arg1	characteristics					570:584	Structural characteristics	559:584	Structural characteristics	559:584	Structural characteristics were elucidated by spectral and chemical analysis.
36214541	7	15	theme	main	641:644	arg1	linkage					674:680	the linkage	670:680	the linkage of β-(1→4)-D-Manp	670:698	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	7	15	theme	main	641:644	arg1	structures					646:655	The main structures	637:655	The main structures of DOPs	637:663	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	4	16	from	studies	341:347	arg1	relationship					375:386	the structure-activity relationship	352:386	the structure-activity relationship of DOP	352:393	However, studies on the structure-activity relationship of DOP are rarely reported.
36214541	0	17	from	relationship	63:74	arg1	Lindl					121:125	Dendrobium catenatum Lindl	100:125	Dendrobium catenatum Lindl	100:125	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	5	18	theme	molecular	541:549	arg1	weight					551:556	molecular weight	541:556	molecular weight	541:556	Here, two polysaccharides named DOP-1 and DOP-2 were obtained, which differed in the ratio of monosaccharide composition and molecular weight.
36214541	5	19	theme	weight	551:556	arg1	ratio					501:505	the ratio	497:505	the ratio of monosaccharide composition and molecular weight	497:556	Here, two polysaccharides named DOP-1 and DOP-2 were obtained, which differed in the ratio of monosaccharide composition and molecular weight.
36214541	0	20	theme	Structural	0:9	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation	0:26	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	8	21	theme	phenolic	1030:1037	arg1	content					1039:1045	higher phenolic content	1023:1045	higher phenolic content	1023:1045	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	1	22	theme	Dendrobium	128:137	arg1	Lindl					149:153	Dendrobium catenatum Lindl	128:153	Dendrobium catenatum Lindl.	128:154	Dendrobium catenatum Lindl.
36214541	0	23	theme	structure-antioxidant	32:52	arg1	relationship					63:74	structure-antioxidant activity relationship	32:74	structure-antioxidant activity relationship	32:74	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	3	24	theme	extensive	307:315	arg1	bioactivities					317:329	extensive bioactivities	307:329	extensive bioactivities	307:329	(DOP) exhibited extensive bioactivities.
36214541	8	25	theme	higher	1023:1028	arg1	content					1039:1045	higher phenolic content	1023:1045	higher phenolic content	1023:1045	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	1	26	theme	catenatum	139:147	arg1	Lindl					149:153	Dendrobium catenatum Lindl	128:153	Dendrobium catenatum Lindl.	128:154	Dendrobium catenatum Lindl.
36214541	2	27	from	Lindl	284:288	arg1	food					200:203	a functional food	187:203	a functional food	187:203	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	2	27	from	Lindl	284:288	arg1	polysaccharides					242:256	polysaccharides	242:256	polysaccharides from Dendrobium catenatum Lindl	242:288	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	2	27	from	Lindl	284:288	arg1	medicine					229:236	traditional Chinese medicine	209:236	traditional Chinese medicine	209:236	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	0	28	from	Lindl	121:125	arg1	relationship					63:74	structure-antioxidant activity relationship	32:74	structure-antioxidant activity relationship	32:74	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	0	28	from	Lindl	121:125	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation	0:26	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	0	28	from	Lindl	121:125	arg1	polysaccharides					79:93	polysaccharides	79:93	polysaccharides from Dendrobium catenatum Lindl	79:125	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	7	29	theme	3-O-acetylated	728:741	arg1	groups					743:748	some attached 2-O- or 3-O-acetylated groups	706:748	some attached 2-O- or 3-O-acetylated groups	706:748	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	2	30	theme	catenatum	274:282	arg1	Lindl					284:288	Dendrobium catenatum Lindl	263:288	Dendrobium catenatum Lindl	263:288	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	5	31	theme	monosaccharide	510:523	arg1	composition					525:535	monosaccharide composition	510:535	monosaccharide composition	510:535	Here, two polysaccharides named DOP-1 and DOP-2 were obtained, which differed in the ratio of monosaccharide composition and molecular weight.
36214541	0	32	theme	activity	54:61	arg1	relationship					63:74	structure-antioxidant activity relationship	32:74	structure-antioxidant activity relationship	32:74	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	8	33	theme	DPPH	769:772	arg1	assays					819:824	the DPPH, hydroxyl and superoxide radicals scavenging assays	765:824	the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2	765:843	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	2	34	theme	functional	189:198	arg1	food					200:203	a functional food	187:203	a functional food	187:203	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	2	35	theme	Dendrobium	263:272	arg1	Lindl					284:288	Dendrobium catenatum Lindl	263:288	Dendrobium catenatum Lindl	263:288	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	4	36	theme	DOP	391:393	arg1	relationship					375:386	the structure-activity relationship	352:386	the structure-activity relationship of DOP	352:393	However, studies on the structure-activity relationship of DOP are rarely reported.
36214541	0	37	theme	polysaccharides	79:93	arg1	relationship					63:74	structure-antioxidant activity relationship	32:74	structure-antioxidant activity relationship	32:74	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	0	37	theme	polysaccharides	79:93	arg1	characterisation					11:26	Structural characterisation	0:26	Structural characterisation	0:26	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	6	38	theme	spectral	605:612	arg1	analysis					627:634	spectral and chemical analysis	605:634	spectral and chemical analysis	605:634	Structural characteristics were elucidated by spectral and chemical analysis.
36214541	8	39	theme	lower	936:940	arg1	weight					952:957	its lower molecular weight	932:957	its lower molecular weight	932:957	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	6	40	theme	chemical	618:625	arg1	analysis					627:634	spectral and chemical analysis	605:634	spectral and chemical analysis	605:634	Structural characteristics were elucidated by spectral and chemical analysis.
36214541	8	41	theme	hydroxyl	775:782	arg1	assays					819:824	the DPPH, hydroxyl and superoxide radicals scavenging assays	765:824	the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2	765:843	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	9	42	theme	food	1144:1147	arg1	industries					1149:1158	the pharmaceutical and food industries	1121:1158	the pharmaceutical and food industries	1121:1158	Our results provide a more accurate basis for the application of DOPs in the pharmaceutical and food industries.
36214541	7	43	theme	attached	711:718	arg1	groups					743:748	some attached 2-O- or 3-O-acetylated groups	706:748	some attached 2-O- or 3-O-acetylated groups	706:748	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	9	44	theme	accurate	1075:1082	arg1	basis					1084:1088	a more accurate basis	1068:1088	a more accurate basis for the application of DOPs in the pharmaceutical and food industries	1068:1158	Our results provide a more accurate basis for the application of DOPs in the pharmaceutical and food industries.
36214541	8	45	theme	DOP-2	839:843	arg1	assays					819:824	the DPPH, hydroxyl and superoxide radicals scavenging assays	765:824	the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2	765:843	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	7	46	theme	β-	685:686	arg1	-D-Manp					692:698	β-(1→4)-D-Manp	685:698	β-(1→4)-D-Manp	685:698	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	2	47	theme	Chinese	221:227	arg1	medicine					229:236	traditional Chinese medicine	209:236	traditional Chinese medicine	209:236	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	8	48	theme	molecular	942:950	arg1	weight					952:957	its lower molecular weight	932:957	its lower molecular weight	932:957	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	49	theme	mannose	967:973	arg1	proportion					975:984	higher mannose proportion	960:984	higher mannose proportion	960:984	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	9	50	theme	DOPs	1113:1116	arg1	application					1098:1108	the application	1094:1108	the application of DOPs in the pharmaceutical and food industries	1094:1158	Our results provide a more accurate basis for the application of DOPs in the pharmaceutical and food industries.
36214541	7	51	with	linkage	674:680	arg1	groups					743:748	some attached 2-O- or 3-O-acetylated groups	706:748	some attached 2-O- or 3-O-acetylated groups	706:748	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	0	52	theme	Dendrobium	100:109	arg1	Lindl					121:125	Dendrobium catenatum Lindl	100:125	Dendrobium catenatum Lindl	100:125	Structural characterisation and structure-antioxidant activity relationship of polysaccharides from Dendrobium catenatum Lindl.
36214541	2	53	theme	traditional	209:219	arg1	medicine					229:236	traditional Chinese medicine	209:236	traditional Chinese medicine	209:236	has been long used in China as a functional food and traditional Chinese medicine and polysaccharides from Dendrobium catenatum Lindl.
36214541	8	54	theme	higher	960:965	arg1	proportion					975:984	higher mannose proportion	960:984	higher mannose proportion	960:984	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	55	theme	scavenging	808:817	arg1	assays					819:824	the DPPH, hydroxyl and superoxide radicals scavenging assays	765:824	the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2	765:843	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	3	56	dep	exhibited	297:305	arg1	DOP					292:294	DOP	292:294	DOP	292:294	(DOP) exhibited extensive bioactivities.
36214541	7	57	theme	1→4	688:690	arg1	-D-Manp					692:698	β-(1→4)-D-Manp	685:698	β-(1→4)-D-Manp	685:698	The main structures of DOPs were the linkage of β-(1→4)-D-Manp, with some attached 2-O- or 3-O-acetylated groups.
36214541	8	58	theme	superoxide	788:797	arg1	radicals					799:806	superoxide radicals	788:806	superoxide radicals	788:806	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
36214541	8	59	theme	radicals	799:806	arg1	assays					819:824	the DPPH, hydroxyl and superoxide radicals scavenging assays	765:824	the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2	765:843	Additionally, the DPPH, hydroxyl and superoxide radicals scavenging assays of DOP-1 and DOP-2 showed that DOP-2 exhibited the higher antioxidant activity, which might be related to its lower molecular weight, higher mannose proportion and lower degree of acetylation, and higher phenolic content.
35741907	5	0	theme	value-added	947:957	arg1	applications					959:970	new value-added applications	943:970	new value-added applications of coffee cascara	943:988	Their presence can contribute to the design of new value-added applications of coffee cascara.
35741907	6	1	theme	pulp	1071:1074	arg1	samples					1076:1082	the coffee cascara pulp samples	1052:1082	the coffee cascara pulp samples	1052:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	7	2	theme	phytochemical	1319:1331	arg1	composition					1333:1343	the phytochemical composition	1315:1343	the phytochemical composition of coffee cascara	1315:1361	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	0	3	from	Characterization	12:27	arg1	Samples					93:99	Diverse Coffee Cascara Samples	70:99	Diverse Coffee Cascara Samples	70:99	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	5	4	theme	applications	959:970	arg1	design					933:938	the design	929:938	the design of new value-added applications of coffee cascara	929:988	Their presence can contribute to the design of new value-added applications of coffee cascara.
35741907	6	5	theme	cascara	1063:1069	arg1	samples					1076:1082	the coffee cascara pulp samples	1052:1082	the coffee cascara pulp samples	1052:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	7	6	theme	analysis	1229:1236	arg1	results					1238:1244	principal component analysis results	1209:1244	principal component analysis results	1209:1244	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	3	7	from	Phytochemicals	544:557	arg1	samples					590:596	20 different coffee cascara samples	562:596	20 different coffee cascara samples	562:596	Phytochemicals in 20 different coffee cascara samples were ultrasonically extracted and analyzed by HPLC-UV and HPLC-MS/MS.
35741907	3	8	theme	cascara	582:588	arg1	samples					590:596	20 different coffee cascara samples	562:596	20 different coffee cascara samples	562:596	Phytochemicals in 20 different coffee cascara samples were ultrasonically extracted and analyzed by HPLC-UV and HPLC-MS/MS.
35741907	6	9	theme	coffee	1056:1061	arg1	samples					1076:1082	the coffee cascara pulp samples	1052:1082	the coffee cascara pulp samples	1052:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	7	10	theme	principal	1209:1217	arg1	results					1238:1244	principal component analysis results	1209:1244	principal component analysis results	1209:1244	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	4	11	theme	unknown	763:769	arg1	Da					786:787	337 Da	782:787	337 Da	782:787	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	11	theme	unknown	763:769	arg1	tautomers					771:779	two still unknown tautomers	753:779	two still unknown tautomers (337 Da)	753:788	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	7	12	theme	coffee	1261:1266	arg1	cultivar					1268:1275	coffee cultivar	1261:1275	coffee cultivar	1261:1275	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	7	13	theme	coffee	1348:1353	arg1	cascara					1355:1361	coffee cascara	1348:1361	coffee cascara	1348:1361	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	3	14	theme	different	565:573	arg1	samples					590:596	20 different coffee cascara samples	562:596	20 different coffee cascara samples	562:596	Phytochemicals in 20 different coffee cascara samples were ultrasonically extracted and analyzed by HPLC-UV and HPLC-MS/MS.
35741907	4	15	theme	first	711:715	arg1	time					717:720	the first time	707:720	the first time	707:720	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	0	16	from	Polysaccharides	51:65	arg1	Samples					93:99	Diverse Coffee Cascara Samples	70:99	Diverse Coffee Cascara Samples	70:99	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	2	17	theme	better	499:504	arg1	evaluation					506:515	the better evaluation	495:515	the better evaluation of potential applications	495:541	This work aimed to provide an improved understanding of phytochemicals and polysaccharides in coffee cascara in order to offer information for the better evaluation of potential applications.
35741907	3	18	theme	coffee	575:580	arg1	samples					590:596	20 different coffee cascara samples	562:596	20 different coffee cascara samples	562:596	Phytochemicals in 20 different coffee cascara samples were ultrasonically extracted and analyzed by HPLC-UV and HPLC-MS/MS.
35741907	1	19	theme	first	189:193	arg1	by-product					216:225	the first and most significant by-product	185:225	the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts	185:349	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	1	19	theme	first	189:193	arg1	cascara					174:180	Coffee cascara	167:180	Coffee cascara	167:180	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	2	20	theme	applications	530:541	arg1	evaluation					506:515	the better evaluation	495:515	the better evaluation of potential applications	495:541	This work aimed to provide an improved understanding of phytochemicals and polysaccharides in coffee cascara in order to offer information for the better evaluation of potential applications.
35741907	7	21	theme	external	1407:1414	arg1	environment					1416:1426	the external environment	1403:1426	the external environment	1403:1426	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	0	22	theme	Preliminary	0:10	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.	0:165	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	4	23	dep	glycosides	815:824	arg1	glycosides					815:824	two dihydroflavonol glycosides	795:824	two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside)	795:893	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	23	dep	glycosides	815:824	arg1	glycoside					844:852	dihydromyricetin glycoside	827:852	dihydromyricetin glycoside	827:852	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	23	dep	glycosides	815:824	arg1	rhamnosylglycoside					875:892	dihydromyricetin rhamnosylglycoside	858:892	dihydromyricetin rhamnosylglycoside	858:892	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	0	24	theme	Phytochemicals	32:45	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.	0:165	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	1	25	theme	urgent	295:300	arg1	priority					302:309	an urgent priority	292:309	an urgent priority to reduce harmful environmental impacts	292:349	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	8	26	theme	same	1559:1562	arg1	cultivar					1564:1571	the same cultivar	1555:1571	the same cultivar	1555:1571	It is suggested that processing method should be carefully designed when generating coffee cascara from the same cultivar and country/farm.
35741907	1	27	theme	significant	204:214	arg1	by-product					216:225	the first and most significant by-product	185:225	the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts	185:349	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	1	27	theme	significant	204:214	arg1	cascara					174:180	Coffee cascara	167:180	Coffee cascara	167:180	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	6	28	theme	neutral	1180:1186	arg1	sugars					1188:1193	minor neutral sugars	1174:1193	minor neutral sugars	1174:1193	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	4	29	theme	dihydroflavonol	799:813	arg1	glycosides					815:824	two dihydroflavonol glycosides	795:824	two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside)	795:893	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	29	theme	dihydroflavonol	799:813	arg1	glycoside					844:852	dihydromyricetin glycoside	827:852	dihydromyricetin glycoside	827:852	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	29	theme	dihydroflavonol	799:813	arg1	rhamnosylglycoside					875:892	dihydromyricetin rhamnosylglycoside	858:892	dihydromyricetin rhamnosylglycoside	858:892	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	8	30	theme	processing	1472:1481	arg1	method					1483:1488	processing method	1472:1488	processing method	1472:1488	It is suggested that processing method should be carefully designed when generating coffee cascara from the same cultivar and country/farm.
35741907	2	31	theme	coffee	446:451	arg1	cascara					453:459	coffee cascara	446:459	coffee cascara	446:459	This work aimed to provide an improved understanding of phytochemicals and polysaccharides in coffee cascara in order to offer information for the better evaluation of potential applications.
35741907	0	32	theme	Polysaccharides	51:65	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.	0:165	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	6	33	theme	polysaccharides	1024:1038	arg1	characterization					1000:1015	Chemical characterization	991:1015	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples	991:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	0	34	theme	Compounds	156:164	arg1	Discovery					137:145	Discovery	137:145	Discovery	137:145	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	0	34	theme	Compounds	156:164	arg1	Quantification					118:131	Quantification	118:131	Quantification	118:131	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	0	34	theme	Compounds	156:164	arg1	Identification					102:115	Identification	102:115	Identification	102:115	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	0	35	from	Samples	93:99	arg1	Characterization					12:27	Preliminary Characterization	0:27	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.	0:165	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	4	36	theme	dihydromyricetin	858:873	arg1	glycosides					815:824	two dihydroflavonol glycosides	795:824	two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside)	795:893	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	36	theme	dihydromyricetin	858:873	arg1	rhamnosylglycoside					875:892	dihydromyricetin rhamnosylglycoside	858:892	dihydromyricetin rhamnosylglycoside	858:892	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	7	37	theme	component	1219:1227	arg1	results					1238:1244	principal component analysis results	1209:1244	principal component analysis results	1209:1244	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	0	38	theme	Novel	150:154	arg1	Compounds					156:164	Novel Compounds	150:164	Novel Compounds	150:164	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	4	39	theme	novel	673:677	arg1	compounds					679:687	Four novel compounds	668:687	Four novel compounds	668:687	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	2	40	theme	polysaccharides	427:441	arg1	understanding					391:403	an improved understanding	379:403	an improved understanding of phytochemicals and polysaccharides	379:441	This work aimed to provide an improved understanding of phytochemicals and polysaccharides in coffee cascara in order to offer information for the better evaluation of potential applications.
35741907	0	41	theme	Diverse	70:76	arg1	Samples					93:99	Diverse Coffee Cascara Samples	70:99	Diverse Coffee Cascara Samples	70:99	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	1	42	theme	harmful	321:327	arg1	impacts					343:349	harmful environmental impacts	321:349	harmful environmental impacts	321:349	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	6	43	from	characterization	1000:1015	arg1	two					1045:1047	two	1045:1047	two	1045:1047	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	6	43	from	characterization	1000:1015	arg1	samples					1076:1082	the coffee cascara pulp samples	1052:1082	the coffee cascara pulp samples	1052:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	2	44	theme	potential	520:528	arg1	applications					530:541	potential applications	520:541	potential applications	520:541	This work aimed to provide an improved understanding of phytochemicals and polysaccharides in coffee cascara in order to offer information for the better evaluation of potential applications.
35741907	6	45	theme	minor	1174:1178	arg1	sugars					1188:1193	minor neutral sugars	1174:1193	minor neutral sugars	1174:1193	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	6	46	from	two	1045:1047	arg1	characterization					1000:1015	Chemical characterization	991:1015	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples	991:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	6	46	from	two	1045:1047	arg1	polysaccharides					1024:1038	two polysaccharides	1020:1038	two polysaccharides from two of the coffee cascara pulp samples	1020:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	1	47	theme	environmental	329:341	arg1	impacts					343:349	harmful environmental impacts	321:349	harmful environmental impacts	321:349	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	8	48	theme	coffee	1535:1540	arg1	cascara					1542:1548	coffee cascara	1535:1548	coffee cascara	1535:1548	It is suggested that processing method should be carefully designed when generating coffee cascara from the same cultivar and country/farm.
35741907	7	49	theme	processing	1432:1441	arg1	method					1443:1448	processing method	1432:1448	processing method	1432:1448	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	5	50	theme	coffee	975:980	arg1	cascara					982:988	coffee cascara	975:988	coffee cascara	975:988	Their presence can contribute to the design of new value-added applications of coffee cascara.
35741907	6	51	theme	Chemical	991:998	arg1	characterization					1000:1015	Chemical characterization	991:1015	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples	991:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	7	52	theme	cascara	1355:1361	arg1	composition					1333:1343	the phytochemical composition	1315:1343	the phytochemical composition of coffee cascara	1315:1361	In addition, principal component analysis results indicated that coffee cultivar and/or country significantly impacted the phytochemical composition of coffee cascara, although differences may be reduced by the external environment and processing method.
35741907	5	53	theme	cascara	982:988	arg1	applications					959:970	new value-added applications	943:970	new value-added applications of coffee cascara	943:988	Their presence can contribute to the design of new value-added applications of coffee cascara.
35741907	1	54	theme	coffee	234:239	arg1	industry					252:259	the coffee processing industry	230:259	the coffee processing industry	230:259	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	4	55	attach	isolated	694:701	arg2	compounds					679:687	Four novel compounds	668:687	Four novel compounds	668:687	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	55	attach	isolated	694:701	arg1	glycosides					815:824	two dihydroflavonol glycosides	795:824	two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside)	795:893	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	55	attach	isolated	694:701	arg1	cascara					734:740	coffee cascara	727:740	coffee cascara	727:740	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	55	attach	isolated	694:701	arg1	tautomers					771:779	two still unknown tautomers	753:779	two still unknown tautomers (337 Da)	753:788	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	56	theme	coffee	727:732	arg1	glycosides					815:824	two dihydroflavonol glycosides	795:824	two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside)	795:893	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	56	theme	coffee	727:732	arg1	cascara					734:740	coffee cascara	727:740	coffee cascara	727:740	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	56	theme	coffee	727:732	arg1	tautomers					771:779	two still unknown tautomers	753:779	two still unknown tautomers (337 Da)	753:788	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	1	57	theme	processing	241:250	arg1	industry					252:259	the coffee processing industry	230:259	the coffee processing industry	230:259	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	0	58	theme	Cascara	85:91	arg1	Samples					93:99	Diverse Coffee Cascara Samples	70:99	Diverse Coffee Cascara Samples	70:99	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	5	59	theme	new	943:945	arg1	applications					959:970	new value-added applications	943:970	new value-added applications of coffee cascara	943:988	Their presence can contribute to the design of new value-added applications of coffee cascara.
35741907	0	60	dep	Characterization	12:27	arg1	Discovery					137:145	Discovery	137:145	Discovery	137:145	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	0	60	dep	Characterization	12:27	arg1	Quantification					118:131	Quantification	118:131	Quantification	118:131	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	0	60	dep	Characterization	12:27	arg1	Identification					102:115	Identification	102:115	Identification	102:115	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	2	61	theme	improved	382:389	arg1	understanding					391:403	an improved understanding	379:403	an improved understanding of phytochemicals and polysaccharides	379:441	This work aimed to provide an improved understanding of phytochemicals and polysaccharides in coffee cascara in order to offer information for the better evaluation of potential applications.
35741907	2	62	theme	phytochemicals	408:421	arg1	understanding					391:403	an improved understanding	379:403	an improved understanding of phytochemicals and polysaccharides	379:441	This work aimed to provide an improved understanding of phytochemicals and polysaccharides in coffee cascara in order to offer information for the better evaluation of potential applications.
35741907	1	63	theme	industry	252:259	arg1	by-product					216:225	the first and most significant by-product	185:225	the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts	185:349	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	1	63	theme	industry	252:259	arg1	cascara					174:180	Coffee cascara	167:180	Coffee cascara	167:180	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	0	64	theme	Coffee	78:83	arg1	Samples					93:99	Diverse Coffee Cascara Samples	70:99	Diverse Coffee Cascara Samples	70:99	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	0	65	from	Phytochemicals	32:45	arg1	Samples					93:99	Diverse Coffee Cascara Samples	70:99	Diverse Coffee Cascara Samples	70:99	Preliminary Characterization of Phytochemicals and Polysaccharides in Diverse Coffee Cascara Samples: Identification, Quantification and Discovery of Novel Compounds.
35741907	4	66	theme	dihydromyricetin	827:842	arg1	glycosides					815:824	two dihydroflavonol glycosides	795:824	two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside)	795:893	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	4	66	theme	dihydromyricetin	827:842	arg1	glycoside					844:852	dihydromyricetin glycoside	827:852	dihydromyricetin glycoside	827:852	Four novel compounds were isolated for the first time from coffee cascara, including two still unknown tautomers (337 Da), and two dihydroflavonol glycosides (dihydromyricetin glycoside and dihydromyricetin rhamnosylglycoside).
35741907	1	67	theme	Coffee	167:172	arg1	by-product					216:225	the first and most significant by-product	185:225	the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts	185:349	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	1	67	theme	Coffee	167:172	arg1	cascara					174:180	Coffee cascara	167:180	Coffee cascara	167:180	Coffee cascara is the first and most significant by-product of the coffee processing industry, whose valorization has become an urgent priority to reduce harmful environmental impacts.
35741907	6	68	theme	samples	1076:1082	arg1	two					1045:1047	two	1045:1047	two	1045:1047	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
35741907	6	68	theme	samples	1076:1082	arg1	samples					1076:1082	the coffee cascara pulp samples	1052:1082	the coffee cascara pulp samples	1052:1082	Chemical characterization of two polysaccharides from two of the coffee cascara pulp samples showed that they were mainly composed of homogalacturonan, with rhamnose and arabinose as minor neutral sugars.
36742005	6	0	theme	crosslinking	1372:1383	arg1	process					1355:1361	a process	1353:1361	a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA)	1353:1458	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	4	1	theme	best	977:980	arg1	rate					990:993	the best release rate	973:993	the best release rate of about 70%	973:1006	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	12	2	theme	obvious	2276:2282	arg1	sensitivity					2287:2297	obvious pH sensitivity	2276:2297	obvious pH sensitivity to the release of NG	2276:2318	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	9	3	theme	final-release	2036:2048	arg1	degree					2061:2066	the highest final-release completion degree	2024:2066	the highest final-release completion degree in SIF	2024:2073	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	8	4	theme	silica/naringenin	1734:1750	arg1	NG/MSN					1767:1772	NG/MSN	1767:1772	NG/MSN	1767:1772	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	4	theme	silica/naringenin	1734:1750	arg1	nanoparticles					1752:1764	mesoporous silica/naringenin nanoparticles	1723:1764	mesoporous silica/naringenin nanoparticles (NG/MSN)	1723:1773	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	12	5	dep	Conclusion	2226:2235	arg1	showed					2269:2274	showed	2269:2274	showed obvious pH sensitivity to the release of NG	2269:2318	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	12	5	dep	Conclusion	2226:2235	arg1	had					2341:2343	had	2341:2343	had a good sustained release effect on NG	2341:2381	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	4	6	theme	colonic	795:801	arg1	fluid					803:807	Simulated colonic fluid	785:807	Simulated colonic fluid (SCF)	785:813	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	6	theme	colonic	795:801	arg1	SCF					810:812	SCF	810:812	SCF	810:812	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	12	7	theme	release	2362:2368	arg1	effect					2370:2375	a good sustained release effect	2345:2375	a good sustained release effect	2345:2375	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	12	8	theme	sustained	2352:2360	arg1	effect					2370:2375	a good sustained release effect	2345:2375	a good sustained release effect	2345:2375	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	6	9	theme	sodium	1439:1444	arg1	SA					1456:1457	SA	1456:1457	SA	1456:1457	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	6	9	theme	sodium	1439:1444	arg1	alginate					1446:1453	sodium alginate	1439:1453	sodium alginate (SA)	1439:1458	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	2	10	theme	loaded	538:543	arg1	KS/MSN					567:572	KS/MSN	567:572	KS/MSN	567:572	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	2	10	theme	loaded	538:543	arg1	spheres					558:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres	472:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	4	11	theme	%	1006:1006	arg1	rate					990:993	the best release rate	973:993	the best release rate of about 70%	973:1006	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	8	12	theme	gel	1636:1638	arg1	properties					1640:1649	the gel properties	1632:1649	the gel properties of KGM	1632:1656	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	2	13	theme	alginate-mesoporous	511:529	arg1	silica					531:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica	472:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	4	14	theme	SIF	1014:1016	arg1	environment					1018:1028	SIF environment	1014:1028	SIF environment	1014:1028	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	1	15	theme	natural	208:214	arg1	macromolecules					227:240	natural biological macromolecules	208:240	natural biological macromolecules	208:240	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	12	16	theme	KS/NM	2241:2245	arg1	spheres					2261:2267	The KS/NM composite gel spheres	2237:2267	The KS/NM composite gel spheres	2237:2267	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	2	17	theme	gel	554:556	arg1	KS/MSN					567:572	KS/MSN	567:572	KS/MSN	567:572	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	2	17	theme	gel	554:556	arg1	spheres					558:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres	472:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	9	18	theme	highest	2028:2034	arg1	degree					2061:2066	the highest final-release completion degree	2024:2066	the highest final-release completion degree in SIF	2024:2073	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	1	19	theme	active	133:138	arg1	substances					140:149	active substances	133:149	active substances	133:149	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	4	20	theme	simulated	839:847	arg1	SIF					873:875	SIF	873:875	SIF	873:875	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	20	theme	simulated	839:847	arg1	fluid					866:870	simulated small intestinal fluid	839:870	simulated small intestinal fluid (SIF)	839:876	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	21	theme	medium	1857:1862	arg1	environment					1813:1823	the simulated digestive environment	1789:1823	the simulated digestive environment of the gastrointestinal release medium	1789:1862	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	5	22	theme	natural	1203:1209	arg1	KGM					1262:1264	KGM	1262:1264	KGM	1262:1264	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	22	theme	natural	1203:1209	arg1	glucomannan					1249:1259	natural macromolecular polysaccharides konjac glucomannan	1203:1259	natural macromolecular polysaccharides konjac glucomannan (KGM)	1203:1265	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	8	23	theme	KGM	1663:1665	arg1	KGM/SA					1687:1692	KGM/SA	1687:1692	KGM/SA	1687:1692	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	23	theme	KGM	1663:1665	arg1	spheres					1678:1684	KGM and SA gel spheres	1663:1684	KGM and SA gel spheres (KGM/SA)	1663:1693	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	9	24	contain	had	1987:1989	arg2	effect					2013:2018	slow-release effect	2000:2018	slow-release effect	2000:2018	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	9	24	contain	had	1987:1989	arg1	spheres					1979:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	9	24	contain	had	1987:1989	arg2	degree					2061:2066	the highest final-release completion degree	2024:2066	the highest final-release completion degree in SIF	2024:2073	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	5	25	theme	polysaccharides	1226:1240	arg1	KGM					1262:1264	KGM	1262:1264	KGM	1262:1264	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	25	theme	polysaccharides	1226:1240	arg1	glucomannan					1249:1259	natural macromolecular polysaccharides konjac glucomannan	1203:1259	natural macromolecular polysaccharides konjac glucomannan (KGM)	1203:1265	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	0	26	theme	gel	76:78	arg1	spheres					80:86	composite gel spheres	66:86	composite gel spheres	66:86	Construction and sustained release of konjac glucomannan/naringin composite gel spheres.
36742005	8	27	theme	SA	1671:1672	arg1	KGM/SA					1687:1692	KGM/SA	1687:1692	KGM/SA	1687:1692	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	27	theme	SA	1671:1672	arg1	spheres					1678:1684	KGM and SA gel spheres	1663:1684	KGM and SA gel spheres (KGM/SA)	1663:1693	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	4	28	theme	prepared	904:911	arg1	spheres					927:933	the prepared composite gel spheres	900:933	the prepared composite gel spheres	900:933	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	28	theme	prepared	904:911	arg1	pH-sensitive					940:951	pH-sensitive	940:951	pH-sensitive	940:951	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	29	theme	glucomannan/sodium	1883:1900	arg1	silica					1922:1927	Konjac glucomannan/sodium alginate-mesoporous silica	1876:1927	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	9	29	theme	glucomannan/sodium	1883:1900	arg1	KS/NM					1958:1962	KS/NM	1958:1962	KS/NM	1958:1962	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	1	30	theme	human	342:346	arg1	body					348:351	the human body	338:351	the human body	338:351	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	2	31	theme	konjac	485:490	arg1	silica					531:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica	472:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	9	32	theme	silica	1922:1927	arg1	spheres					1979:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	4	33	theme	release	729:735	arg1	rates					737:741	the cumulative release rates	714:741	the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF)	714:813	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	33	theme	release	729:735	arg1	lower					820:824	lower	820:824	lower	820:824	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	34	theme	gastrointestinal	1832:1847	arg1	medium					1857:1862	the gastrointestinal release medium	1828:1862	the gastrointestinal release medium	1828:1862	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	5	35	theme	mesoporous	1174:1183	arg1	MSN					1193:1195	MSN	1193:1195	MSN	1193:1195	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	35	theme	mesoporous	1174:1183	arg1	silica					1185:1190	inorganic nano-materials mesoporous silica	1149:1190	inorganic nano-materials mesoporous silica (MSN)	1149:1196	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	10	36	from	spheres	2119:2125	arg1	release					2080:2086	The release	2076:2086	The release of NG from KS/NM composite gel spheres	2076:2125	The release of NG from KS/NM composite gel spheres showed a slow upward trend.
36742005	9	37	theme	naringin	1936:1943	arg1	spheres					1949:1955	loaded naringin gel spheres	1929:1955	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	10	38	theme	gel	2115:2117	arg1	spheres					2119:2125	KS/NM composite gel spheres	2099:2125	KS/NM composite gel spheres	2099:2125	The release of NG from KS/NM composite gel spheres showed a slow upward trend.
36742005	2	39	theme	sodium	440:445	arg1	AC					457:458	AC	457:458	AC	457:458	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	2	39	theme	sodium	440:445	arg1	alginate					447:454	sodium alginate	440:454	sodium alginate (AC)	440:459	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	12	40	theme	gel	2329:2331	arg1	spheres					2333:2339	the gel spheres	2325:2339	the gel spheres	2325:2339	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	10	41	theme	upward	2141:2146	arg1	trend					2148:2152	a slow upward trend	2134:2152	a slow upward trend	2134:2152	The release of NG from KS/NM composite gel spheres showed a slow upward trend.
36742005	2	42	theme	mesoporous	369:378	arg1	silica					380:385	mesoporous silica	369:385	mesoporous silica (MSN)	369:391	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	2	42	theme	mesoporous	369:378	arg1	MSN					388:390	MSN	388:390	MSN	388:390	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	5	43	theme	gel	1101:1103	arg1	spheres					1105:1111	composite gel spheres	1091:1111	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA)	1031:1120	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	6	44	dep	Results	1311:1317	arg1	showed					1331:1336	showed	1331:1336	showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA)	1331:1458	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	2	45	theme	konjac	411:416	arg1	glucomannan					418:428	konjac glucomannan	411:428	konjac glucomannan (KGM)	411:434	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	2	45	theme	konjac	411:416	arg1	KGM					431:433	KGM	431:433	KGM	431:433	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	0	46	theme	sustained	17:25	arg1	release					27:33	sustained release	17:33	sustained release	17:33	Construction and sustained release of konjac glucomannan/naringin composite gel spheres.
36742005	7	47	theme	good	1540:1543	arg1	compatibility					1545:1557	good compatibility	1540:1557	good compatibility	1540:1557	Naringin (NG) and mesoporous silica (MSN) were successfully compounded and had good compatibility.
36742005	4	48	theme	gel	923:925	arg1	spheres					927:933	the prepared composite gel spheres	900:933	the prepared composite gel spheres	900:933	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	48	theme	gel	923:925	arg1	pH-sensitive					940:951	pH-sensitive	940:951	pH-sensitive	940:951	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	5	49	dep	Methods	1031:1037	arg1	alginate					1082:1089	The pH-sensitive konjac glucomannan/sodium alginate	1039:1089	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA)	1031:1120	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	49	dep	Methods	1031:1037	arg1	spheres					1105:1111	composite gel spheres	1091:1111	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA)	1031:1120	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	9	50	theme	composite	1965:1973	arg1	spheres					1979:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	5	51	theme	konjac	1056:1061	arg1	alginate					1082:1089	The pH-sensitive konjac glucomannan/sodium alginate	1039:1089	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA)	1031:1120	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	1	52	theme	different	312:320	arg1	environments					322:333	different environments	312:333	different environments of the human body	312:351	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	1	53	used	used	246:249	arg2	macromolecules					227:240	natural biological macromolecules	208:240	natural biological macromolecules	208:240	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	12	54	theme	NG	2317:2318	arg1	release					2306:2312	the release	2302:2312	the release of NG	2302:2318	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	4	55	theme	simulated	751:759	arg1	SGF					776:778	SGF	776:778	SGF	776:778	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	55	theme	simulated	751:759	arg1	fluid					769:773	simulated gastric fluid	751:773	simulated gastric fluid (SGF)	751:779	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	10	56	theme	NG	2091:2092	arg1	release					2080:2086	The release	2076:2086	The release of NG from KS/NM composite gel spheres	2076:2125	The release of NG from KS/NM composite gel spheres showed a slow upward trend.
36742005	1	57	theme	biological	216:225	arg1	macromolecules					227:240	natural biological macromolecules	208:240	natural biological macromolecules	208:240	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	12	58	theme	pH	2284:2285	arg1	sensitivity					2287:2297	obvious pH sensitivity	2276:2297	obvious pH sensitivity to the release of NG	2276:2318	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	3	59	theme	release	622:628	arg1	properties					630:639	release properties	622:639	release properties	622:639	On this basis, the structure, morphology, and release properties of the composite gel spheres were characterized.
36742005	4	60	theme	fluid	769:773	arg1	rates					737:741	the cumulative release rates	714:741	the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF)	714:813	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	60	theme	fluid	769:773	arg1	lower					820:824	lower	820:824	lower	820:824	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	61	dep	silica	1922:1927	arg1	spheres					1949:1955	loaded naringin gel spheres	1929:1955	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	5	62	theme	nano-materials	1159:1172	arg1	MSN					1193:1195	MSN	1193:1195	MSN	1193:1195	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	62	theme	nano-materials	1159:1172	arg1	silica					1185:1190	inorganic nano-materials mesoporous silica	1149:1190	inorganic nano-materials mesoporous silica (MSN)	1149:1196	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	9	63	from	effect	2013:2018	arg1	SIF					2071:2073	SIF	2071:2073	SIF	2071:2073	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	3	64	theme	spheres	662:668	arg1	morphology					606:615	morphology	606:615	morphology	606:615	On this basis, the structure, morphology, and release properties of the composite gel spheres were characterized.
36742005	3	64	theme	spheres	662:668	arg1	structure					595:603	structure	595:603	structure	595:603	On this basis, the structure, morphology, and release properties of the composite gel spheres were characterized.
36742005	3	64	theme	spheres	662:668	arg1	properties					630:639	release properties	622:639	release properties	622:639	On this basis, the structure, morphology, and release properties of the composite gel spheres were characterized.
36742005	8	65	theme	mesoporous	1723:1732	arg1	NG/MSN					1767:1772	NG/MSN	1767:1772	NG/MSN	1767:1772	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	65	theme	mesoporous	1723:1732	arg1	nanoparticles					1752:1764	mesoporous silica/naringenin nanoparticles	1723:1764	mesoporous silica/naringenin nanoparticles (NG/MSN)	1723:1773	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	66	theme	gel	1564:1566	arg1	diagram					1583:1589	The gel microstructure diagram	1560:1589	The gel microstructure diagram	1560:1589	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	4	67	theme	release	982:988	arg1	rate					990:993	the best release rate	973:993	the best release rate of about 70%	973:1006	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	12	68	theme	gel	2257:2259	arg1	spheres					2261:2267	The KS/NM composite gel spheres	2237:2267	The KS/NM composite gel spheres	2237:2267	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	3	69	theme	composite	648:656	arg1	spheres					662:668	the composite gel spheres	644:668	the composite gel spheres	644:668	On this basis, the structure, morphology, and release properties of the composite gel spheres were characterized.
36742005	6	70	theme	ionic	1366:1370	arg1	crosslinking					1372:1383	ionic crosslinking	1366:1383	ionic crosslinking	1366:1383	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	1	71	theme	active	259:264	arg1	substances					266:275	active substances	259:275	active substances	259:275	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	9	72	theme	completion	2050:2059	arg1	degree					2061:2066	the highest final-release completion degree	2024:2066	the highest final-release completion degree in SIF	2024:2073	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	1	73	theme	side	176:179	arg1	effects					181:187	the toxic and side effects	162:187	the toxic and side effects on the human body	162:205	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	4	74	theme	Simulated	785:793	arg1	fluid					803:807	Simulated colonic fluid	785:807	Simulated colonic fluid (SCF)	785:813	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	74	theme	Simulated	785:793	arg1	SCF					810:812	SCF	810:812	SCF	810:812	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	75	theme	fluid	803:807	arg1	rates					737:741	the cumulative release rates	714:741	the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF)	714:813	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	75	theme	fluid	803:807	arg1	lower					820:824	lower	820:824	lower	820:824	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	76	theme	simulated	1793:1801	arg1	environment					1813:1823	the simulated digestive environment	1789:1823	the simulated digestive environment of the gastrointestinal release medium	1789:1862	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	10	77	theme	composite	2105:2113	arg1	spheres					2119:2125	KS/NM composite gel spheres	2099:2125	KS/NM composite gel spheres	2099:2125	The release of NG from KS/NM composite gel spheres showed a slow upward trend.
36742005	8	78	theme	MSN	1619:1621	arg1	addition					1607:1614	the addition	1603:1614	the addition of MSN	1603:1621	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	1	79	theme	human	196:200	arg1	body					202:205	the human body	192:205	the human body	192:205	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	9	80	dep	effect	2013:2018	arg1	the					1991:1993	the	1991:1993	the	1991:1993	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	4	81	theme	small	849:853	arg1	SIF					873:875	SIF	873:875	SIF	873:875	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	81	theme	small	849:853	arg1	fluid					866:870	simulated small intestinal fluid	839:870	simulated small intestinal fluid (SIF)	839:876	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	82	theme	environment	1813:1823	arg1	study					1780:1784	The study	1776:1784	The study of the simulated digestive environment of the gastrointestinal release medium	1776:1862	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	11	83	theme	composite	2185:2193	arg1	spheres					2199:2205	KS/NM composite gel spheres	2179:2205	KS/NM composite gel spheres	2179:2205	The results showed that KS/NM composite gel spheres were pH-sensitive.
36742005	2	84	theme	glucomannan/sodium	492:509	arg1	silica					531:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica	472:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	12	85	theme	composite	2247:2255	arg1	spheres					2261:2267	The KS/NM composite gel spheres	2237:2267	The KS/NM composite gel spheres	2237:2267	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	12	86	theme	good	2347:2350	arg1	effect					2370:2375	a good sustained release effect	2345:2375	a good sustained release effect	2345:2375	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	5	87	theme	pH-sensitive	1043:1054	arg1	alginate					1082:1089	The pH-sensitive konjac glucomannan/sodium alginate	1039:1089	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA)	1031:1120	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	4	88	theme	intestinal	855:864	arg1	SIF					873:875	SIF	873:875	SIF	873:875	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	88	theme	intestinal	855:864	arg1	fluid					866:870	simulated small intestinal fluid	839:870	simulated small intestinal fluid (SIF)	839:876	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	89	theme	release	1849:1855	arg1	medium					1857:1862	the gastrointestinal release medium	1828:1862	the gastrointestinal release medium	1828:1862	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	8	90	theme	KGM	1654:1656	arg1	properties					1640:1649	the gel properties	1632:1649	the gel properties of KGM	1632:1656	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	1	91	theme	substances	140:149	arg1	bioavailability					114:128	the bioavailability	110:128	the bioavailability of active substances	110:149	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	7	92	theme	mesoporous	1479:1488	arg1	MSN					1498:1500	MSN	1498:1500	MSN	1498:1500	Naringin (NG) and mesoporous silica (MSN) were successfully compounded and had good compatibility.
36742005	7	92	theme	mesoporous	1479:1488	arg1	silica					1490:1495	mesoporous silica	1479:1495	mesoporous silica (MSN)	1479:1501	Naringin (NG) and mesoporous silica (MSN) were successfully compounded and had good compatibility.
36742005	2	93	theme	naringin	545:552	arg1	KS/MSN					567:572	KS/MSN	567:572	KS/MSN	567:572	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	2	93	theme	naringin	545:552	arg1	spheres					558:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres	472:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	10	94	theme	KS/NM	2099:2103	arg1	spheres					2119:2125	KS/NM composite gel spheres	2099:2125	KS/NM composite gel spheres	2099:2125	The release of NG from KS/NM composite gel spheres showed a slow upward trend.
36742005	9	95	theme	digestive	1803:1811	arg1	environment					1813:1823	the simulated digestive environment	1789:1823	the simulated digestive environment of the gastrointestinal release medium	1789:1862	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	5	96	theme	macromolecular	1211:1224	arg1	KGM					1262:1264	KGM	1262:1264	KGM	1262:1264	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	96	theme	macromolecular	1211:1224	arg1	glucomannan					1249:1259	natural macromolecular polysaccharides konjac glucomannan	1203:1259	natural macromolecular polysaccharides konjac glucomannan (KGM)	1203:1265	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	9	97	theme	Konjac	1876:1881	arg1	silica					1922:1927	Konjac glucomannan/sodium alginate-mesoporous silica	1876:1927	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	9	97	theme	Konjac	1876:1881	arg1	KS/NM					1958:1962	KS/NM	1958:1962	KS/NM	1958:1962	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	3	98	dep	structure	595:603	arg1	the					591:593	the	591:593	the	591:593	On this basis, the structure, morphology, and release properties of the composite gel spheres were characterized.
36742005	1	99	from	effects	181:187	arg1	body					202:205	the human body	192:205	the human body	192:205	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	1	100	theme	toxic	166:170	arg1	effects					181:187	the toxic and side effects	162:187	the toxic and side effects on the human body	162:205	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	2	101	theme	silica	531:536	arg1	KS/MSN					567:572	KS/MSN	567:572	KS/MSN	567:572	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	2	101	theme	silica	531:536	arg1	spheres					558:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres	472:564	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	12	102	contain	had	2341:2343	arg2	effect					2370:2375	a good sustained release effect	2345:2375	a good sustained release effect	2345:2375	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	12	102	contain	had	2341:2343	arg1	spheres					2333:2339	the gel spheres	2325:2339	the gel spheres	2325:2339	Conclusion The KS/NM composite gel spheres showed obvious pH sensitivity to the release of NG, and the gel spheres had a good sustained release effect on NG.
36742005	9	103	theme	alginate-mesoporous	1902:1920	arg1	silica					1922:1927	Konjac glucomannan/sodium alginate-mesoporous silica	1876:1927	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	9	103	theme	alginate-mesoporous	1902:1920	arg1	KS/NM					1958:1962	KS/NM	1958:1962	KS/NM	1958:1962	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	1	104	theme	body	348:351	arg1	environments					322:333	different environments	312:333	different environments of the human body	312:351	Objective To improve the bioavailability of active substances and reduce the toxic and side effects on the human body, natural biological macromolecules are used to load active substances and control their release speed in different environments of the human body.
36742005	8	105	theme	gel	1674:1676	arg1	KGM/SA					1687:1692	KGM/SA	1687:1692	KGM/SA	1687:1692	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	105	theme	gel	1674:1676	arg1	spheres					1678:1684	KGM and SA gel spheres	1663:1684	KGM and SA gel spheres (KGM/SA)	1663:1693	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	2	106	theme	pH-sensitive	472:483	arg1	silica					531:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica	472:536	pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN)	472:573	In this study, mesoporous silica (MSN) was combined with konjac glucomannan (KGM) and sodium alginate (AC) to prepare pH-sensitive konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/MSN).
36742005	9	107	theme	loaded	1929:1934	arg1	spheres					1949:1955	loaded naringin gel spheres	1929:1955	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	4	108	theme	cumulative	718:727	arg1	rates					737:741	the cumulative release rates	714:741	the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF)	714:813	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	108	theme	cumulative	718:727	arg1	lower					820:824	lower	820:824	lower	820:824	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	109	theme	gel	1945:1947	arg1	spheres					1949:1955	loaded naringin gel spheres	1929:1955	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	5	110	theme	sodium	1271:1276	arg1	SA					1288:1289	SA	1288:1289	SA	1288:1289	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	110	theme	sodium	1271:1276	arg1	alginate					1278:1285	sodium alginate	1271:1285	sodium alginate (SA)	1271:1290	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	9	111	from	degree	2061:2066	arg1	SIF					2071:2073	SIF	2071:2073	SIF	2071:2073	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	9	112	theme	slow-release	2000:2011	arg1	effect					2013:2018	slow-release effect	2000:2018	slow-release effect	2000:2018	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	10	113	theme	slow	2136:2139	arg1	trend					2148:2152	a slow upward trend	2134:2152	a slow upward trend	2134:2152	The release of NG from KS/NM composite gel spheres showed a slow upward trend.
36742005	5	114	theme	konjac	1242:1247	arg1	KGM					1262:1264	KGM	1262:1264	KGM	1262:1264	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	114	theme	konjac	1242:1247	arg1	glucomannan					1249:1259	natural macromolecular polysaccharides konjac glucomannan	1203:1259	natural macromolecular polysaccharides konjac glucomannan (KGM)	1203:1265	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	8	115	theme	good	1699:1702	arg1	compatibility					1704:1716	good compatibility	1699:1716	good compatibility	1699:1716	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	11	116	theme	gel	2195:2197	arg1	spheres					2199:2205	KS/NM composite gel spheres	2179:2205	KS/NM composite gel spheres	2179:2205	The results showed that KS/NM composite gel spheres were pH-sensitive.
36742005	11	117	theme	KS/NM	2179:2183	arg1	spheres					2199:2205	KS/NM composite gel spheres	2179:2205	KS/NM composite gel spheres	2179:2205	The results showed that KS/NM composite gel spheres were pH-sensitive.
36742005	0	118	theme	konjac	38:43	arg1	Construction					0:11	Construction	0:11	Construction	0:11	Construction and sustained release of konjac glucomannan/naringin composite gel spheres.
36742005	0	118	theme	konjac	38:43	arg1	release					27:33	sustained release	17:33	sustained release	17:33	Construction and sustained release of konjac glucomannan/naringin composite gel spheres.
36742005	4	119	theme	composite	913:921	arg1	spheres					927:933	the prepared composite gel spheres	900:933	the prepared composite gel spheres	900:933	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	119	theme	composite	913:921	arg1	pH-sensitive					940:951	pH-sensitive	940:951	pH-sensitive	940:951	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	9	120	theme	gel	1975:1977	arg1	spheres					1979:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres	1876:1985	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	0	121	theme	composite	66:74	arg1	spheres					80:86	composite gel spheres	66:86	composite gel spheres	66:86	Construction and sustained release of konjac glucomannan/naringin composite gel spheres.
36742005	5	122	theme	glucomannan/sodium	1063:1080	arg1	alginate					1082:1089	The pH-sensitive konjac glucomannan/sodium alginate	1039:1089	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA)	1031:1120	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	123	theme	composite	1091:1099	arg1	spheres					1105:1111	composite gel spheres	1091:1111	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA)	1031:1120	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	9	124	theme	best	1995:1998	arg1	effect					2013:2018	slow-release effect	2000:2018	slow-release effect	2000:2018	The study of the simulated digestive environment of the gastrointestinal release medium showed that Konjac glucomannan/sodium alginate-mesoporous silica loaded naringin gel spheres (KS/NM) composite gel spheres had the best slow-release effect and the highest final-release completion degree in SIF.
36742005	6	125	theme	konjac	1410:1415	arg1	KGM					1430:1432	KGM	1430:1432	KGM	1430:1432	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	6	125	theme	konjac	1410:1415	arg1	glucomannan					1417:1427	konjac glucomannan	1410:1427	konjac glucomannan (KGM)	1410:1433	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	5	126	theme	inorganic	1149:1157	arg1	MSN					1193:1195	MSN	1193:1195	MSN	1193:1195	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	5	126	theme	inorganic	1149:1157	arg1	silica					1185:1190	inorganic nano-materials mesoporous silica	1149:1190	inorganic nano-materials mesoporous silica (MSN)	1149:1196	Methods The pH-sensitive konjac glucomannan/sodium alginate composite gel spheres (KGM/SA) were prepared by combining inorganic nano-materials mesoporous silica (MSN) with natural macromolecular polysaccharides konjac glucomannan (KGM) and sodium alginate (SA) and characterized.
36742005	6	127	theme	entanglement	1389:1400	arg1	process					1355:1361	a process	1353:1361	a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA)	1353:1458	Results The results showed that there was a process of ionic crosslinking and entanglement between konjac glucomannan (KGM) and sodium alginate (SA).
36742005	3	128	theme	gel	658:660	arg1	spheres					662:668	the composite gel spheres	644:668	the composite gel spheres	644:668	On this basis, the structure, morphology, and release properties of the composite gel spheres were characterized.
36742005	4	129	theme	gastric	761:767	arg1	SGF					776:778	SGF	776:778	SGF	776:778	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	4	129	theme	gastric	761:767	arg1	fluid					769:773	simulated gastric fluid	751:773	simulated gastric fluid (SGF)	751:779	The results showed that the cumulative release rates of both simulated gastric fluid (SGF) and Simulated colonic fluid (SCF) were lower than that of simulated small intestinal fluid (SIF), which indicated that the prepared composite gel spheres were pH-sensitive to SIF and obtained the best release rate of about 70% under SIF environment.
36742005	8	130	theme	microstructure	1568:1581	arg1	diagram					1583:1589	The gel microstructure diagram	1560:1589	The gel microstructure diagram	1560:1589	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	131	contain	had	1695:1697	arg2	compatibility					1704:1716	good compatibility	1699:1716	good compatibility	1699:1716	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	131	contain	had	1695:1697	arg1	KGM/SA					1687:1692	KGM/SA	1687:1692	KGM/SA	1687:1692	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
36742005	8	131	contain	had	1695:1697	arg1	spheres					1678:1684	KGM and SA gel spheres	1663:1684	KGM and SA gel spheres (KGM/SA)	1663:1693	The gel microstructure diagram showed that the addition of MSN improved the gel properties of KGM, and KGM and SA gel spheres (KGM/SA) had good compatibility with mesoporous silica/naringenin nanoparticles (NG/MSN).
35983486	5	0	theme	content	1102:1108	arg1	detection					1077:1085	the detection	1073:1085	the detection of total sugar content	1073:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	3	1	theme	present	498:504	arg1	study					506:510	the present study	494:510	the present study	494:510	Therefore, the present study focused to unveil the proposed problem.
35983486	0	2	theme	content	78:84	arg1	analysis					54:61	quantitative analysis	41:61	quantitative analysis of total sugar content by phenol-sulfuric acid method	41:115	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	5	3	theme	monosaccharides	884:898	arg1	capabilities					858:869	the color-rendering capabilities	838:869	the color-rendering capabilities of 10 common monosaccharides	838:898	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	0	4	theme	acid	105:108	arg1	method					110:115	phenol-sulfuric acid method	89:115	phenol-sulfuric acid method	89:115	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	0	5	from	Effects	0:6	arg1	analysis					54:61	quantitative analysis	41:61	quantitative analysis of total sugar content by phenol-sulfuric acid method	41:115	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	3	6	theme	proposed	534:541	arg1	problem					543:549	the proposed problem	530:549	the proposed problem	530:549	Therefore, the present study focused to unveil the proposed problem.
35983486	4	7	theme	linearity	649:657	arg1	conditions					594:603	colorimetric conditions	581:603	colorimetric conditions	581:603	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	7	theme	linearity	649:657	arg1	range					659:663	linearity range	649:663	linearity range	649:663	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	7	theme	linearity	649:657	arg1	length					627:632	optimal wave length	614:632	optimal wave length of absorption	614:646	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	8	theme	conditions	594:603	arg1	optimization					565:576	the optimization	561:576	the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature	561:700	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	0	9	theme	phenol-sulfuric	89:103	arg1	method					110:115	phenol-sulfuric acid method	89:115	phenol-sulfuric acid method	89:115	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	7	10	theme	convenient	1443:1452	arg1	method					1454:1459	this convenient method	1438:1459	this convenient method	1438:1459	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	5	11	theme	common	877:882	arg1	monosaccharides					884:898	10 common monosaccharides	874:898	10 common monosaccharides	874:898	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	4	12	theme	colorimetric	581:592	arg1	time					681:684	color reaction time	666:684	color reaction time	666:684	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	12	theme	colorimetric	581:592	arg1	conditions					594:603	colorimetric conditions	581:603	colorimetric conditions	581:603	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	12	theme	colorimetric	581:592	arg1	range					659:663	linearity range	649:663	linearity range	649:663	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	12	theme	colorimetric	581:592	arg1	length					627:632	optimal wave length	614:632	optimal wave length of absorption	614:646	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	12	theme	colorimetric	581:592	arg1	temperature					690:700	temperature	690:700	temperature	690:700	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	7	13	theme	method	1454:1459	arg1	application					1423:1433	the application	1419:1433	the application of this convenient method in the polysaccharides study	1419:1488	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	4	14	theme	convenient	759:768	arg1	way					783:785	a convenient and accurate way	757:785	a convenient and accurate way for the total sugar content analysis	757:822	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	14	theme	convenient	759:768	arg1	method					746:751	the phenol-sulfuric acid method	721:751	the phenol-sulfuric acid method	721:751	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	5	15	theme	color-rendering	842:856	arg1	capabilities					858:869	the color-rendering capabilities	838:869	the color-rendering capabilities of 10 common monosaccharides	838:898	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	1	16	theme	common	165:170	arg1	methods					172:178	the most common methods	156:178	the most common methods applied to the analysis of total sugar content during polysaccharides study	156:254	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	7	17	from	application	1423:1433	arg1	study					1484:1488	the polysaccharides study	1464:1488	the polysaccharides study	1464:1488	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	1	18	theme	methods	172:178	arg1	methods					172:178	the most common methods	156:178	the most common methods applied to the analysis of total sugar content during polysaccharides study	156:254	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	1	18	theme	methods	172:178	arg1	one					149:151	one	149:151	one	149:151	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	5	19	theme	total	1090:1094	arg1	content					1102:1108	total sugar content	1090:1108	total sugar content	1090:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	7	20	theme	experimental	1272:1283	arg1	method					1294:1299	a reliable experimental research method	1261:1299	a reliable experimental research method	1261:1299	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	7	21	theme	theoretical	1239:1249	arg1	basis					1251:1255	a scientific theoretical basis	1226:1255	a scientific theoretical basis	1226:1255	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	2	22	theme	real	371:374	arg1	content					388:394	the real total sugar content	367:394	the real total sugar content	367:394	However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples.
35983486	7	23	theme	reliable	1263:1270	arg1	method					1294:1299	a reliable experimental research method	1261:1299	a reliable experimental research method	1261:1299	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	6	24	theme	key	1125:1127	arg1	points					1129:1134	the key points	1121:1134	the key points during the application of phenol-sulfuric acid method	1121:1188	Moreover, the key points during the application of phenol-sulfuric acid method were suggested.
35983486	7	25	theme	accurate	1309:1316	arg1	determination					1318:1330	the accurate determination	1305:1330	the accurate determination of total sugar content	1305:1353	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	0	26	theme	composition	26:36	arg1	Effects					0:6	Effects	0:6	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method	0:115	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	7	27	theme	total	1335:1339	arg1	content					1347:1353	total sugar content	1335:1353	total sugar content	1335:1353	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	7	28	theme	sugar	1341:1345	arg1	content					1347:1353	total sugar content	1335:1353	total sugar content	1335:1353	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	4	29	theme	acid	741:744	arg1	way					783:785	a convenient and accurate way	757:785	a convenient and accurate way for the total sugar content analysis	757:822	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	29	theme	acid	741:744	arg1	method					746:751	the phenol-sulfuric acid method	721:751	the phenol-sulfuric acid method	721:751	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	5	30	theme	correction	950:959	arg1	factor					961:966	a relative correction factor	939:966	a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content	939:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	2	31	theme	acidic	413:418	arg1	monosaccharides					420:434	acidic monosaccharides	413:434	acidic monosaccharides	413:434	However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples.
35983486	4	32	theme	phenol-sulfuric	725:739	arg1	way					783:785	a convenient and accurate way	757:785	a convenient and accurate way for the total sugar content analysis	757:822	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	32	theme	phenol-sulfuric	725:739	arg1	method					746:751	the phenol-sulfuric acid method	721:751	the phenol-sulfuric acid method	721:751	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	33	theme	wave	622:625	arg1	length					627:632	optimal wave length	614:632	optimal wave length of absorption	614:646	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	0	34	theme	quantitative	41:52	arg1	analysis					54:61	quantitative analysis	41:61	quantitative analysis of total sugar content by phenol-sulfuric acid method	41:115	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	2	35	theme	acid	330:333	arg1	method					335:340	the phenol-sulfuric acid method	310:340	the phenol-sulfuric acid method	310:340	However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples.
35983486	7	36	theme	scientific	1228:1237	arg1	basis					1251:1255	a scientific theoretical basis	1226:1255	a scientific theoretical basis	1226:1255	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	4	37	theme	total	795:799	arg1	content					807:813	the total sugar content	791:813	the total sugar content analysis	791:822	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	38	theme	absorption	637:646	arg1	length					627:632	optimal wave length	614:632	optimal wave length of absorption	614:646	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	2	39	theme	polysaccharides	458:472	arg1	samples					474:480	the polysaccharides samples	454:480	the polysaccharides samples	454:480	However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples.
35983486	7	40	theme	phenol-sulfuric	1362:1376	arg1	method					1383:1388	the phenol-sulfuric acid method	1358:1388	the phenol-sulfuric acid method	1358:1388	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	1	41	theme	total	207:211	arg1	content					219:225	total sugar content	207:225	total sugar content during polysaccharides study	207:254	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	5	42	from	deviation	1060:1068	arg1	detection					1077:1085	the detection	1073:1085	the detection of total sugar content	1073:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	7	43	theme	content	1347:1353	arg1	determination					1318:1330	the accurate determination	1305:1330	the accurate determination of total sugar content	1305:1353	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	4	44	theme	content	807:813	arg1	analysis					815:822	the total sugar content analysis	791:822	the total sugar content analysis	791:822	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	1	45	theme	Phenol-sulfuric	118:132	arg1	method					139:144	Phenol-sulfuric acid method	118:144	Phenol-sulfuric acid method	118:144	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	2	46	theme	sugar	382:386	arg1	content					388:394	the real total sugar content	367:394	the real total sugar content	367:394	However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples.
35983486	7	47	theme	polysaccharides	1468:1482	arg1	study					1484:1488	the polysaccharides study	1464:1488	the polysaccharides study	1464:1488	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	4	48	theme	sugar	801:805	arg1	content					807:813	the total sugar content	791:813	the total sugar content analysis	791:822	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	1	49	theme	acid	134:137	arg1	method					139:144	Phenol-sulfuric acid method	118:144	Phenol-sulfuric acid method	118:144	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	6	50	theme	phenol-sulfuric	1162:1176	arg1	method					1183:1188	phenol-sulfuric acid method	1162:1188	phenol-sulfuric acid method	1162:1188	Moreover, the key points during the application of phenol-sulfuric acid method were suggested.
35983486	5	51	theme	relative	992:999	arg1	monosaccharide					977:990	each monosaccharide	972:990	each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content	972:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	4	52	theme	accurate	774:781	arg1	way					783:785	a convenient and accurate way	757:785	a convenient and accurate way for the total sugar content analysis	757:822	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	52	theme	accurate	774:781	arg1	method					746:751	the phenol-sulfuric acid method	721:751	the phenol-sulfuric acid method	721:751	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	2	53	theme	phenol-sulfuric	314:328	arg1	method					335:340	the phenol-sulfuric acid method	310:340	the phenol-sulfuric acid method	310:340	However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples.
35983486	5	54	theme	relative	941:948	arg1	factor					961:966	a relative correction factor	939:966	a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content	939:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	1	55	theme	sugar	213:217	arg1	content					219:225	total sugar content	207:225	total sugar content during polysaccharides study	207:254	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	5	56	theme	main	1040:1043	arg1	glucose					1004:1010	glucose	1004:1010	glucose	1004:1010	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	5	56	theme	main	1040:1043	arg1	reason					1045:1050	the main reason	1036:1050	the main reason for the deviation in the detection of total sugar content	1036:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	6	57	theme	method	1183:1188	arg1	application					1147:1157	the application	1143:1157	the application of phenol-sulfuric acid method	1143:1188	Moreover, the key points during the application of phenol-sulfuric acid method were suggested.
35983486	7	58	theme	acid	1378:1381	arg1	method					1383:1388	the phenol-sulfuric acid method	1358:1388	the phenol-sulfuric acid method	1358:1388	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	1	59	theme	content	219:225	arg1	analysis					195:202	the analysis	191:202	the analysis of total sugar content during polysaccharides study	191:254	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35983486	0	60	theme	sugar	72:76	arg1	content					78:84	total sugar content	66:84	total sugar content	66:84	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	7	61	theme	research	1285:1292	arg1	method					1294:1299	a reliable experimental research method	1261:1299	a reliable experimental research method	1261:1299	This study provides a scientific theoretical basis and a reliable experimental research method for the accurate determination of total sugar content by the phenol-sulfuric acid method, and which will also promote the application of this convenient method in the polysaccharides study.
35983486	6	62	theme	acid	1178:1181	arg1	method					1183:1188	phenol-sulfuric acid method	1162:1188	phenol-sulfuric acid method	1162:1188	Moreover, the key points during the application of phenol-sulfuric acid method were suggested.
35983486	4	63	theme	reaction	672:679	arg1	time					681:684	color reaction time	666:684	color reaction time	666:684	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	63	theme	reaction	672:679	arg1	conditions					594:603	colorimetric conditions	581:603	colorimetric conditions	581:603	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	63	theme	reaction	672:679	arg1	length					627:632	optimal wave length	614:632	optimal wave length of absorption	614:646	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	2	64	theme	total	376:380	arg1	content					388:394	the real total sugar content	367:394	the real total sugar content	367:394	However, it was found that the results obtained from the phenol-sulfuric acid method was generally lower than the real total sugar content, especially when acidic monosaccharides were contained in the polysaccharides samples.
35983486	4	65	theme	optimal	614:620	arg1	length					627:632	optimal wave length	614:632	optimal wave length of absorption	614:646	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	0	66	theme	total	66:70	arg1	content					78:84	total sugar content	66:84	total sugar content	66:84	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	5	67	theme	sugar	1096:1100	arg1	content					1102:1108	total sugar content	1090:1108	total sugar content	1090:1108	In addition, the color-rendering capabilities of 10 common monosaccharides were systematically analyzed to obtain a relative correction factor for each monosaccharide relative to glucose, which was proved to be the main reason for the deviation in the detection of total sugar content.
35983486	0	68	theme	monosaccharide	11:24	arg1	composition					26:36	monosaccharide composition	11:36	monosaccharide composition	11:36	Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method.
35983486	4	69	theme	color	666:670	arg1	time					681:684	color reaction time	666:684	color reaction time	666:684	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	69	theme	color	666:670	arg1	conditions					594:603	colorimetric conditions	581:603	colorimetric conditions	581:603	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	4	69	theme	color	666:670	arg1	length					627:632	optimal wave length	614:632	optimal wave length of absorption	614:646	Based on the optimization of colorimetric conditions, such as optimal wave length of absorption, linearity range, color reaction time and temperature, it indicated that the phenol-sulfuric acid method was a convenient and accurate way for the total sugar content analysis.
35983486	1	70	theme	polysaccharides	234:248	arg1	study					250:254	polysaccharides study	234:254	polysaccharides study	234:254	Phenol-sulfuric acid method is one of the most common methods applied to the analysis of total sugar content during polysaccharides study.
35742014	8	0	theme	related	1676:1682	arg1	metabolites					1664:1674	23 differential metabolites	1648:1674	23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination	1648:1799	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	5	1	from	liver	1126:1130	arg1	prevention					1088:1097	prevention	1088:1097	prevention of pathological changes in liver, kidney and proximal colon tissue	1088:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	1	from	liver	1126:1130	arg1	actions					1183:1189	suppressive actions	1171:1189	suppressive actions against oxidative stress	1171:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	1	from	liver	1126:1130	arg1	accumulation					1011:1022	fat accumulation	1007:1022	fat accumulation	1007:1022	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	1	from	liver	1126:1130	arg1	regulation					1060:1069	regulation	1060:1069	regulation of dyslipidemia	1060:1085	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	1	from	liver	1126:1130	arg1	weight					991:996	body weight	986:996	body weight (BW)	986:1001	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	1	from	liver	1126:1130	arg1	BW					999:1000	BW	999:1000	BW	999:1000	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	1	from	liver	1126:1130	arg1	improvement					1025:1035	improvement	1025:1035	improvement in insulin resistance	1025:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	8	2	theme	alba	1754:1757	arg1	fruit					1762:1766	Morus alba L. fruit	1748:1766	Morus alba L. fruit	1748:1766	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	1	3	theme	health-promoting	242:257	arg1	effects					259:265	potential health-promoting effects	232:265	potential health-promoting effects against metabolic syndrome (MetS)	232:299	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	5	4	from	changes	1115:1121	arg1	kidney					1133:1138	kidney	1133:1138	kidney	1133:1138	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	4	from	changes	1115:1121	arg1	liver					1126:1130	liver	1126:1130	liver	1126:1130	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	4	from	changes	1115:1121	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	5	from	reductions	972:981	arg1	prevention					1088:1097	prevention	1088:1097	prevention of pathological changes in liver, kidney and proximal colon tissue	1088:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	5	from	reductions	972:981	arg1	weight					991:996	body weight	986:996	body weight (BW)	986:1001	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	5	from	reductions	972:981	arg1	accumulation					1011:1022	fat accumulation	1007:1022	fat accumulation	1007:1022	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	5	from	reductions	972:981	arg1	regulation					1060:1069	regulation	1060:1069	regulation of dyslipidemia	1060:1085	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	5	from	reductions	972:981	arg1	actions					1183:1189	suppressive actions	1171:1189	suppressive actions against oxidative stress	1171:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	5	from	reductions	972:981	arg1	BW					999:1000	BW	999:1000	BW	999:1000	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	5	from	reductions	972:981	arg1	improvement					1025:1035	improvement	1025:1035	improvement in insulin resistance	1025:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	7	6	theme	opportunistic	1467:1479	arg1	Fusobacterium					1547:1559	Fusobacterium	1547:1559	Fusobacterium	1547:1559	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	7	6	theme	opportunistic	1467:1479	arg1	Faecalibacterium					1526:1541	Faecalibacterium	1526:1541	Faecalibacterium	1526:1541	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	7	6	theme	opportunistic	1467:1479	arg1	pathogens					1481:1489	opportunistic pathogens	1467:1489	opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium	1467:1559	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	7	6	theme	opportunistic	1467:1479	arg1	Prevotella_2					1499:1510	Prevotella_2	1499:1510	Prevotella_2	1499:1510	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	4	7	theme	metabolite	801:810	arg1	profiles					812:819	metabolite profiles	801:819	metabolite profiles	801:819	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	9	8	theme	microbiota	1965:1974	arg1	composition					1976:1986	the gut microbiota composition	1957:1986	the gut microbiota composition	1957:1986	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	4	9	theme	Morus	649:653	arg1	fruit					663:667	Morus alba L. fruit	649:667	Morus alba L. fruit against MetS	649:680	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	5	10	theme	colon	1153:1157	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	4	11	theme	L.	660:661	arg1	fruit					663:667	Morus alba L. fruit	649:667	Morus alba L. fruit against MetS	649:680	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	5	12	from	kidney	1133:1138	arg1	prevention					1088:1097	prevention	1088:1097	prevention of pathological changes in liver, kidney and proximal colon tissue	1088:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	12	from	kidney	1133:1138	arg1	actions					1183:1189	suppressive actions	1171:1189	suppressive actions against oxidative stress	1171:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	12	from	kidney	1133:1138	arg1	accumulation					1011:1022	fat accumulation	1007:1022	fat accumulation	1007:1022	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	12	from	kidney	1133:1138	arg1	regulation					1060:1069	regulation	1060:1069	regulation of dyslipidemia	1060:1085	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	12	from	kidney	1133:1138	arg1	weight					991:996	body weight	986:996	body weight (BW)	986:1001	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	12	from	kidney	1133:1138	arg1	BW					999:1000	BW	999:1000	BW	999:1000	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	12	from	kidney	1133:1138	arg1	improvement					1025:1035	improvement	1025:1035	improvement in insulin resistance	1025:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	1	13	theme	medicinal	184:192	arg1	fruit					175:179	Morus alba L. fruit	161:179	Morus alba L. fruit	161:179	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	1	13	theme	medicinal	184:192	arg1	fruit					205:209	a medicinal and edible fruit	182:209	a medicinal and edible fruit in East Asia	182:222	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	7	14	theme	genera	1373:1378	arg1	Muribaculum					1380:1390	beneficial bacterial genera Muribaculum	1352:1390	beneficial bacterial genera Muribaculum	1352:1390	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	9	15	theme	metabolite	1998:2007	arg1	profiles					2009:2016	fecal metabolite profiles	1992:2016	fecal metabolite profiles	1992:2016	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	3	16	theme	MetS	544:547	arg1	development					529:539	the development	525:539	the development of MetS	525:547	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	1	17	theme	edible	198:203	arg1	fruit					175:179	Morus alba L. fruit	161:179	Morus alba L. fruit	161:179	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	1	17	theme	edible	198:203	arg1	fruit					205:209	a medicinal and edible fruit	182:209	a medicinal and edible fruit in East Asia	182:222	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	7	18	theme	beneficial	1352:1361	arg1	Muribaculum					1380:1390	beneficial bacterial genera Muribaculum	1352:1390	beneficial bacterial genera Muribaculum	1352:1390	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	5	19	from	tissue	1159:1164	arg1	prevention					1088:1097	prevention	1088:1097	prevention of pathological changes in liver, kidney and proximal colon tissue	1088:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	19	from	tissue	1159:1164	arg1	actions					1183:1189	suppressive actions	1171:1189	suppressive actions against oxidative stress	1171:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	19	from	tissue	1159:1164	arg1	accumulation					1011:1022	fat accumulation	1007:1022	fat accumulation	1007:1022	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	19	from	tissue	1159:1164	arg1	regulation					1060:1069	regulation	1060:1069	regulation of dyslipidemia	1060:1085	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	19	from	tissue	1159:1164	arg1	weight					991:996	body weight	986:996	body weight (BW)	986:1001	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	19	from	tissue	1159:1164	arg1	BW					999:1000	BW	999:1000	BW	999:1000	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	19	from	tissue	1159:1164	arg1	improvement					1025:1035	improvement	1025:1035	improvement in insulin resistance	1025:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	1	20	from	fruit	205:209	arg1	Asia					219:222	East Asia	214:222	East Asia	214:222	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	4	21	theme	diet	694:697	arg1	mice					709:712	high-fat diet (HFD)-fed mice	685:712	high-fat diet (HFD)-fed mice	685:712	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	5	22	theme	Morus	881:885	arg1	fruit					895:899	Morus alba L. fruit	881:899	Morus alba L. fruit	881:899	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	23	from	actions	1183:1189	arg1	kidney					1133:1138	kidney	1133:1138	kidney	1133:1138	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	23	from	actions	1183:1189	arg1	resistance					1048:1057	insulin resistance	1040:1057	insulin resistance	1040:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	23	from	actions	1183:1189	arg1	liver					1126:1130	liver	1126:1130	liver	1126:1130	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	23	from	actions	1183:1189	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	24	theme	fat	1007:1009	arg1	accumulation					1011:1022	fat accumulation	1007:1022	fat accumulation	1007:1022	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	25	theme	L.	892:893	arg1	fruit					895:899	Morus alba L. fruit	881:899	Morus alba L. fruit	881:899	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	26	from	accumulation	1011:1022	arg1	kidney					1133:1138	kidney	1133:1138	kidney	1133:1138	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	26	from	accumulation	1011:1022	arg1	resistance					1048:1057	insulin resistance	1040:1057	insulin resistance	1040:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	26	from	accumulation	1011:1022	arg1	liver					1126:1130	liver	1126:1130	liver	1126:1130	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	26	from	accumulation	1011:1022	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	27	theme	dyslipidemia	1074:1085	arg1	prevention					1088:1097	prevention	1088:1097	prevention of pathological changes in liver, kidney and proximal colon tissue	1088:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	27	theme	dyslipidemia	1074:1085	arg1	actions					1183:1189	suppressive actions	1171:1189	suppressive actions against oxidative stress	1171:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	27	theme	dyslipidemia	1074:1085	arg1	accumulation					1011:1022	fat accumulation	1007:1022	fat accumulation	1007:1022	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	27	theme	dyslipidemia	1074:1085	arg1	regulation					1060:1069	regulation	1060:1069	regulation of dyslipidemia	1060:1085	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	27	theme	dyslipidemia	1074:1085	arg1	weight					991:996	body weight	986:996	body weight (BW)	986:1001	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	27	theme	dyslipidemia	1074:1085	arg1	BW					999:1000	BW	999:1000	BW	999:1000	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	27	theme	dyslipidemia	1074:1085	arg1	improvement					1025:1035	improvement	1025:1035	improvement in insulin resistance	1025:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	1	28	theme	Morus	161:165	arg1	fruit					175:179	Morus alba L. fruit	161:179	Morus alba L. fruit	161:179	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	1	28	theme	Morus	161:165	arg1	fruit					205:209	a medicinal and edible fruit	182:209	a medicinal and edible fruit in East Asia	182:222	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	0	29	theme	Diet-Induced	76:87	arg1	Syndrome					99:106	High-Fat Diet-Induced Metabolic Syndrome	67:106	High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile	67:158	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	1	30	theme	L.	172:173	arg1	fruit					175:179	Morus alba L. fruit	161:179	Morus alba L. fruit	161:179	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	1	30	theme	L.	172:173	arg1	fruit					205:209	a medicinal and edible fruit	182:209	a medicinal and edible fruit in East Asia	182:222	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	2	31	theme	Morus	392:396	arg1	fruit					406:410	Morus alba L. fruit	392:410	Morus alba L. fruit	392:410	However, both the protective effects and mechanisms of different fractions extracted from Morus alba L. fruit against MetS remain unclear.
35742014	5	32	from	improvement	1025:1035	arg1	kidney					1133:1138	kidney	1133:1138	kidney	1133:1138	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	32	from	improvement	1025:1035	arg1	resistance					1048:1057	insulin resistance	1040:1057	insulin resistance	1040:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	32	from	improvement	1025:1035	arg1	liver					1126:1130	liver	1126:1130	liver	1126:1130	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	32	from	improvement	1025:1035	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	0	33	theme	alba	43:46	arg1	Fruit					51:55	Morus alba L. Fruit	37:55	Morus alba L. Fruit	37:55	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	5	34	theme	MetS	956:959	arg1	reductions					972:981	reductions	972:981	reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress	972:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	34	theme	MetS	956:959	arg1	parameters					942:951	the traditional pharmacodynamic parameters	910:951	the traditional pharmacodynamic parameters	910:951	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	4	35	theme	-fed	704:707	arg1	mice					709:712	high-fat diet (HFD)-fed mice	685:712	high-fat diet (HFD)-fed mice	685:712	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	1	36	theme	metabolic	275:283	arg1	MetS					295:298	MetS	295:298	MetS	295:298	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	1	36	theme	metabolic	275:283	arg1	syndrome					285:292	metabolic syndrome	275:292	metabolic syndrome (MetS)	275:299	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	5	37	theme	traditional	914:924	arg1	reductions					972:981	reductions	972:981	reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress	972:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	37	theme	traditional	914:924	arg1	parameters					942:951	the traditional pharmacodynamic parameters	910:951	the traditional pharmacodynamic parameters	910:951	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	9	38	theme	alba	1897:1900	arg1	fruit					1905:1909	Morus alba L. fruit	1891:1909	Morus alba L. fruit	1891:1909	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	2	39	theme	L.	403:404	arg1	fruit					406:410	Morus alba L. fruit	392:410	Morus alba L. fruit	392:410	However, both the protective effects and mechanisms of different fractions extracted from Morus alba L. fruit against MetS remain unclear.
35742014	0	40	theme	Morus	37:41	arg1	Fruit					51:55	Morus alba L. Fruit	37:55	Morus alba L. Fruit	37:55	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	6	41	from	polyphenols	1255:1265	arg1	combination					1290:1300	combination	1290:1300	combination	1290:1300	In particular, the group treated with polyphenols and polysaccharides in combination showed better efficacy.
35742014	5	42	theme	pathological	1102:1113	arg1	changes					1115:1121	pathological changes	1102:1121	pathological changes in liver, kidney and proximal colon tissue	1102:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	4	43	from	role	596:599	arg1	mice					709:712	high-fat diet (HFD)-fed mice	685:712	high-fat diet (HFD)-fed mice	685:712	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	0	44	theme	L.	48:49	arg1	Fruit					51:55	Morus alba L. Fruit	37:55	Morus alba L. Fruit	37:55	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	6	45	from	polysaccharides	1271:1285	arg1	combination					1290:1300	combination	1290:1300	combination	1290:1300	In particular, the group treated with polyphenols and polysaccharides in combination showed better efficacy.
35742014	8	46	theme	fecal	1613:1617	arg1	profiles					1630:1637	fecal metabolite profiles	1613:1637	fecal metabolite profiles	1613:1637	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	5	47	theme	suppressive	1171:1181	arg1	actions					1183:1189	suppressive actions	1171:1189	suppressive actions against oxidative stress	1171:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	9	48	theme	HFD-fed	1930:1936	arg1	mice					1938:1941	HFD-fed mice	1930:1941	HFD-fed mice	1930:1941	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	4	49	attach	derived	636:642	arg2	polyphenols					604:614	polyphenols	604:614	polyphenols	604:614	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	4	49	attach	derived	636:642	arg1	fruit					663:667	Morus alba L. fruit	649:667	Morus alba L. fruit against MetS	649:680	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	4	50	theme	remodeling	760:769	arg1	effects					771:777	remodeling effects	760:777	remodeling effects on gut microbiota and metabolite profiles	760:819	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	2	51	theme	different	357:365	arg1	fractions					367:375	different fractions	357:375	different fractions extracted from Morus alba L. fruit	357:410	However, both the protective effects and mechanisms of different fractions extracted from Morus alba L. fruit against MetS remain unclear.
35742014	1	52	theme	potential	232:240	arg1	effects					259:265	potential health-promoting effects	232:265	potential health-promoting effects against metabolic syndrome (MetS)	232:299	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	8	53	theme	Morus	1748:1752	arg1	fruit					1762:1766	Morus alba L. fruit	1748:1766	Morus alba L. fruit	1748:1766	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	7	54	theme	various	1444:1450	arg1	degrees					1452:1458	various degrees	1444:1458	various degrees	1444:1458	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	9	55	theme	gut	1961:1963	arg1	composition					1976:1986	the gut microbiota composition	1957:1986	the gut microbiota composition	1957:1986	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	8	56	theme	L.	1759:1760	arg1	fruit					1762:1766	Morus alba L. fruit	1748:1766	Morus alba L. fruit	1748:1766	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	5	57	attach	derived	868:874	arg2	polyphenols					836:846	polyphenols	836:846	polyphenols	836:846	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	57	attach	derived	868:874	arg1	fruit					895:899	Morus alba L. fruit	881:899	Morus alba L. fruit	881:899	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	8	58	with	treatments	1687:1696	arg1	polysaccharides					1719:1733	polysaccharides	1719:1733	polysaccharides	1719:1733	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	8	58	with	treatments	1687:1696	arg1	polyphenols					1703:1713	polyphenols	1703:1713	polyphenols	1703:1713	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	7	59	theme	Lachnospiraceae_NK4A136_group	1396:1424	arg1	abundance					1339:1347	The relative abundance	1326:1347	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group	1326:1424	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	9	60	theme	fecal	1992:1996	arg1	profiles					2009:2016	fecal metabolite profiles	1992:2016	fecal metabolite profiles	1992:2016	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	4	61	theme	alba	655:658	arg1	fruit					663:667	Morus alba L. fruit	649:667	Morus alba L. fruit against MetS	649:680	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	6	62	theme	better	1309:1314	arg1	efficacy					1316:1323	better efficacy	1309:1323	better efficacy	1309:1323	In particular, the group treated with polyphenols and polysaccharides in combination showed better efficacy.
35742014	7	63	theme	Muribaculum	1380:1390	arg1	abundance					1339:1347	The relative abundance	1326:1347	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group	1326:1424	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	4	64	theme	polyphenols	604:614	arg1	role					596:599	the potential role	582:599	the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice	582:712	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	9	65	attach	derived	1878:1884	arg2	polyphenols					1846:1856	polyphenols	1846:1856	polyphenols	1846:1856	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	9	65	attach	derived	1878:1884	arg1	fruit					1905:1909	Morus alba L. fruit	1891:1909	Morus alba L. fruit	1891:1909	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	5	66	from	weight	991:996	arg1	kidney					1133:1138	kidney	1133:1138	kidney	1133:1138	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	66	from	weight	991:996	arg1	resistance					1048:1057	insulin resistance	1040:1057	insulin resistance	1040:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	66	from	weight	991:996	arg1	liver					1126:1130	liver	1126:1130	liver	1126:1130	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	66	from	weight	991:996	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	7	67	theme	bacterial	1363:1371	arg1	Muribaculum					1380:1390	beneficial bacterial genera Muribaculum	1352:1390	beneficial bacterial genera Muribaculum	1352:1390	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	4	68	theme	polysaccharides	620:634	arg1	role					596:599	the potential role	582:599	the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice	582:712	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	5	69	theme	insulin	1040:1046	arg1	resistance					1048:1057	insulin resistance	1040:1057	insulin resistance	1040:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	8	70	attach	derived	1735:1741	arg1	fruit					1762:1766	Morus alba L. fruit	1748:1766	Morus alba L. fruit	1748:1766	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	8	70	attach	derived	1735:1741	arg2	polyphenols					1703:1713	polyphenols	1703:1713	polyphenols	1703:1713	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	4	71	theme	high-fat	685:692	arg1	diet					694:697	high-fat diet	685:697	high-fat diet (HFD)-fed mice	685:712	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	4	71	theme	high-fat	685:692	arg1	HFD					700:702	HFD	700:702	HFD	700:702	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	4	72	from	effects	771:777	arg1	profiles					812:819	metabolite profiles	801:819	metabolite profiles	801:819	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	4	72	from	effects	771:777	arg1	microbiota					786:795	gut microbiota	782:795	gut microbiota	782:795	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	5	73	theme	alba	887:890	arg1	fruit					895:899	Morus alba L. fruit	881:899	Morus alba L. fruit	881:899	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	9	74	from	MetS	1922:1925	arg1	mice					1938:1941	HFD-fed mice	1930:1941	HFD-fed mice	1930:1941	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	7	75	theme	relative	1330:1337	arg1	abundance					1339:1347	The relative abundance	1326:1347	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group	1326:1424	The relative abundance of beneficial bacterial genera Muribaculum and Lachnospiraceae_NK4A136_group were increased to various degrees, while opportunistic pathogens such as Prevotella_2, Bacteroides, Faecalibacterium and Fusobacterium were markedly decreased after treatments.
35742014	0	76	theme	High-Fat	67:74	arg1	Syndrome					99:106	High-Fat Diet-Induced Metabolic Syndrome	67:106	High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile	67:158	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	1	77	theme	East	214:217	arg1	Asia					219:222	East Asia	214:222	East Asia	214:222	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	3	78	from	factors	514:520	arg1	development					529:539	the development	525:539	the development of MetS	525:547	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	0	79	theme	Metabolic	89:97	arg1	Syndrome					99:106	High-Fat Diet-Induced Metabolic Syndrome	67:106	High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile	67:158	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	1	80	theme	alba	167:170	arg1	fruit					175:179	Morus alba L. fruit	161:179	Morus alba L. fruit	161:179	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	1	80	theme	alba	167:170	arg1	fruit					205:209	a medicinal and edible fruit	182:209	a medicinal and edible fruit in East Asia	182:222	Morus alba L. fruit, a medicinal and edible fruit in East Asia, showed potential health-promoting effects against metabolic syndrome (MetS).
35742014	5	81	theme	pharmacodynamic	926:940	arg1	reductions					972:981	reductions	972:981	reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress	972:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	81	theme	pharmacodynamic	926:940	arg1	parameters					942:951	the traditional pharmacodynamic parameters	910:951	the traditional pharmacodynamic parameters	910:951	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	0	82	from	Fruit	51:55	arg1	Polysaccharides					16:30	Polysaccharides	16:30	Polysaccharides	16:30	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	0	82	from	Fruit	51:55	arg1	Polyphenols					0:10	Polyphenols	0:10	Polyphenols	0:10	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	0	83	theme	Gut	122:124	arg1	Microbiota					126:135	the Gut Microbiota	118:135	the Gut Microbiota	118:135	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	5	84	from	regulation	1060:1069	arg1	kidney					1133:1138	kidney	1133:1138	kidney	1133:1138	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	84	from	regulation	1060:1069	arg1	resistance					1048:1057	insulin resistance	1040:1057	insulin resistance	1040:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	84	from	regulation	1060:1069	arg1	liver					1126:1130	liver	1126:1130	liver	1126:1130	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	84	from	regulation	1060:1069	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	2	85	theme	fractions	367:375	arg1	mechanisms					343:352	mechanisms	343:352	mechanisms	343:352	However, both the protective effects and mechanisms of different fractions extracted from Morus alba L. fruit against MetS remain unclear.
35742014	2	85	theme	fractions	367:375	arg1	effects					331:337	the protective effects	316:337	the protective effects	316:337	However, both the protective effects and mechanisms of different fractions extracted from Morus alba L. fruit against MetS remain unclear.
35742014	9	86	theme	Morus	1891:1895	arg1	fruit					1905:1909	Morus alba L. fruit	1891:1909	Morus alba L. fruit	1891:1909	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	3	87	theme	gut	459:461	arg1	microbiota					463:472	the gut microbiota	455:472	the gut microbiota	455:472	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	3	87	theme	gut	459:461	arg1	metabolites					482:492	its metabolites	478:492	its metabolites	478:492	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	3	87	theme	gut	459:461	arg1	factors					514:520	key factors	510:520	key factors in the development of MetS	510:547	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	5	88	from	prevention	1088:1097	arg1	kidney					1133:1138	kidney	1133:1138	kidney	1133:1138	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	88	from	prevention	1088:1097	arg1	resistance					1048:1057	insulin resistance	1040:1057	insulin resistance	1040:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	88	from	prevention	1088:1097	arg1	liver					1126:1130	liver	1126:1130	liver	1126:1130	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	88	from	prevention	1088:1097	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	89	theme	proximal	1144:1151	arg1	tissue					1159:1164	proximal colon tissue	1144:1164	proximal colon tissue	1144:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	90	theme	body	986:989	arg1	weight					991:996	body weight	986:996	body weight (BW)	986:1001	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	90	theme	body	986:989	arg1	BW					999:1000	BW	999:1000	BW	999:1000	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	9	91	theme	L.	1902:1903	arg1	fruit					1905:1909	Morus alba L. fruit	1891:1909	Morus alba L. fruit	1891:1909	Altogether, these results demonstrated that polyphenols and polysaccharides derived from Morus alba L. fruit attenuated MetS in HFD-fed mice, and improved the gut microbiota composition and fecal metabolite profiles.
35742014	2	92	theme	alba	398:401	arg1	fruit					406:410	Morus alba L. fruit	392:410	Morus alba L. fruit	392:410	However, both the protective effects and mechanisms of different fractions extracted from Morus alba L. fruit against MetS remain unclear.
35742014	5	93	theme	changes	1115:1121	arg1	prevention					1088:1097	prevention	1088:1097	prevention of pathological changes in liver, kidney and proximal colon tissue	1088:1164	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	93	theme	changes	1115:1121	arg1	actions					1183:1189	suppressive actions	1171:1189	suppressive actions against oxidative stress	1171:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	93	theme	changes	1115:1121	arg1	accumulation					1011:1022	fat accumulation	1007:1022	fat accumulation	1007:1022	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	93	theme	changes	1115:1121	arg1	regulation					1060:1069	regulation	1060:1069	regulation of dyslipidemia	1060:1085	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	93	theme	changes	1115:1121	arg1	weight					991:996	body weight	986:996	body weight (BW)	986:1001	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	93	theme	changes	1115:1121	arg1	BW					999:1000	BW	999:1000	BW	999:1000	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	5	93	theme	changes	1115:1121	arg1	improvement					1025:1035	improvement	1025:1035	improvement in insulin resistance	1025:1057	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
35742014	0	94	theme	Metabolite	141:150	arg1	Profile					152:158	Metabolite Profile	141:158	Metabolite Profile	141:158	Polyphenols and Polysaccharides from Morus alba L. Fruit Attenuate High-Fat Diet-Induced Metabolic Syndrome Modifying the Gut Microbiota and Metabolite Profile.
35742014	8	95	theme	metabolite	1619:1628	arg1	profiles					1630:1637	fecal metabolite profiles	1613:1637	fecal metabolite profiles	1613:1637	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	4	96	theme	gut	782:784	arg1	microbiota					786:795	gut microbiota	782:795	gut microbiota	782:795	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	2	97	theme	protective	320:329	arg1	effects					331:337	the protective effects	316:337	the protective effects	316:337	However, both the protective effects and mechanisms of different fractions extracted from Morus alba L. fruit against MetS remain unclear.
35742014	4	98	theme	potential	586:594	arg1	role					596:599	the potential role	582:599	the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice	582:712	This study aimed to investigate the potential role of polyphenols and polysaccharides derived from Morus alba L. fruit against MetS in high-fat diet (HFD)-fed mice, individually and in combination, focusing on remodeling effects on gut microbiota and metabolite profiles.
35742014	8	99	theme	differential	1651:1662	arg1	metabolites					1664:1674	23 differential metabolites	1648:1674	23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination	1648:1799	Moreover, fecal metabolite profiles revealed 23 differential metabolites related to treatments with polyphenols and polysaccharides derived from Morus alba L. fruit, individually and in combination.
35742014	3	100	theme	key	510:512	arg1	factors					514:520	key factors	510:520	key factors in the development of MetS	510:547	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	3	100	theme	key	510:512	arg1	metabolites					482:492	its metabolites	478:492	its metabolites	478:492	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	3	100	theme	key	510:512	arg1	microbiota					463:472	the gut microbiota	455:472	the gut microbiota	455:472	Additionally, the gut microbiota and its metabolites are regarded as key factors in the development of MetS.
35742014	5	101	theme	oxidative	1199:1207	arg1	stress					1209:1214	oxidative stress	1199:1214	oxidative stress	1199:1214	In the study, polyphenols and polysaccharides derived from Morus alba L. fruit improved the traditional pharmacodynamic parameters of MetS, including reductions in body weight (BW) and fat accumulation, improvement in insulin resistance, regulation of dyslipidemia, prevention of pathological changes in liver, kidney and proximal colon tissue, and suppressive actions against oxidative stress.
37276896	0	0	theme	giant	84:88	arg1	prawn					101:105	giant freshwater prawn	84:105	giant freshwater prawn	84:105	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	5	1	dep	0.20 	655:659	arg1	to					652:653	to	652:653	to	652:653	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	2	2	from	effects	301:307	arg1	response					364:371	innate immune response	350:371	innate immune response	350:371	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	2	2	from	effects	301:307	arg1	axis					388:391	brain-gut axis	378:391	brain-gut axis	378:391	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	2	2	from	effects	301:307	arg1	growth					342:347	growth	342:347	growth	342:347	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	5	3	theme	intestinal	715:724	arg1	tissues					726:732	intestinal tissues	715:732	intestinal tissues	715:732	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	1	4	with	Supplementation	168:182	arg1	polysaccharides					200:214	Astragalus polysaccharides	189:214	Astragalus polysaccharides (APS)	189:220	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	1	4	with	Supplementation	168:182	arg1	APS					217:219	APS	217:219	APS	217:219	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	8	5	theme	protein	1306:1312	arg1	concentration					1314:1326	total protein concentration	1300:1326	total protein concentration	1300:1326	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	8	6	theme	Vibrio	1438:1443	arg1	anguillarum					1445:1455	Vibrio anguillarum	1438:1455	Vibrio anguillarum	1438:1455	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	0	7	theme	prawn	101:105	arg1	growth					47:52	growth	47:52	growth	47:52	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	0	7	theme	prawn	101:105	arg1	response					72:79	innate immune response	58:79	innate immune response	58:79	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	6	8	theme	lower	790:794	arg1	bacteria					829:836	pathogenic intestinal bacteria	807:836	pathogenic intestinal bacteria (Vibrio and Aeromonas)	807:859	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	8	theme	lower	790:794	arg1	amounts					796:802	significantly lower amounts	776:802	significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure	776:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	8	theme	lower	790:794	arg1	structure					906:914	a markedly different microbial community structure	865:914	a markedly different microbial community structure	865:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	8	9	theme	improved	1407:1414	arg1	resistance					1424:1433	improved disease resistance	1407:1433	improved disease resistance to Vibrio anguillarum	1407:1455	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	8	10	theme	phenoloxidase	1329:1341	arg1	activity					1343:1350	phenoloxidase activity	1329:1350	phenoloxidase activity	1329:1350	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	0	11	from	axis	162:165	arg1	Insights					134:141	Insights	134:141	Insights from the brain-gut axis	134:165	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	0	12	theme	freshwater	90:99	arg1	prawn					101:105	giant freshwater prawn	84:105	giant freshwater prawn	84:105	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	7	13	theme	prawns	1076:1081	arg1	brains					1066:1071	the brains	1062:1071	the brains of prawns receiving APS	1062:1095	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	6	14	theme	structure	906:914	arg1	bacteria					829:836	pathogenic intestinal bacteria	807:836	pathogenic intestinal bacteria (Vibrio and Aeromonas)	807:859	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	14	theme	structure	906:914	arg1	amounts					796:802	significantly lower amounts	776:802	significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure	776:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	14	theme	structure	906:914	arg1	structure					906:914	a markedly different microbial community structure	865:914	a markedly different microbial community structure	865:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	9	15	theme	SCFA	1612:1615	arg1	content					1617:1623	the fecal SCFA content	1602:1623	the fecal SCFA content	1602:1623	Thus, dietary APS positively affected the gut-brain axis by altering the microbiota composition, increasing the fecal SCFA content, and enhancing prawn immunity.
37276896	6	16	contain	had	772:774	arg1	Prawns					735:740	Prawns	735:740	Prawns receiving APS supplementation	735:770	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	16	contain	had	772:774	arg2	bacteria					829:836	pathogenic intestinal bacteria	807:836	pathogenic intestinal bacteria (Vibrio and Aeromonas)	807:859	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	16	contain	had	772:774	arg2	structure					906:914	a markedly different microbial community structure	865:914	a markedly different microbial community structure	865:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	16	contain	had	772:774	arg2	amounts					796:802	significantly lower amounts	776:802	significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure	776:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	7	17	dep	acid	986:989	arg1	contents					1040:1047	γ-aminobutyric acid contents	1020:1047	γ-aminobutyric acid contents	1020:1047	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	6	18	theme	APS	752:754	arg1	supplementation					756:770	APS supplementation	752:770	APS supplementation	752:770	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	8	19	dep	function	1260:1267	arg1	such					1270:1273	such	1270:1273	such	1270:1273	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	2	20	theme	brain-gut	378:386	arg1	axis					388:391	brain-gut axis	378:391	brain-gut axis	378:391	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	7	21	theme	Bacillus	1164:1171	arg1	levels					1173:1178	increased Lactobacillus and Bacillus levels	1136:1178	increased Lactobacillus and Bacillus levels	1136:1178	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	9	22	theme	dietary	1500:1506	arg1	APS					1508:1510	dietary APS	1500:1510	dietary APS	1500:1510	Thus, dietary APS positively affected the gut-brain axis by altering the microbiota composition, increasing the fecal SCFA content, and enhancing prawn immunity.
37276896	7	23	theme	Lactobacillus	1146:1158	arg1	levels					1173:1178	increased Lactobacillus and Bacillus levels	1136:1178	increased Lactobacillus and Bacillus levels	1136:1178	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	1	24	theme	Astragalus	189:198	arg1	polysaccharides					200:214	Astragalus polysaccharides	189:214	Astragalus polysaccharides (APS)	189:220	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	1	24	theme	Astragalus	189:198	arg1	APS					217:219	APS	217:219	APS	217:219	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	0	25	theme	Astragalus	8:17	arg1	polysaccharides					19:33	Dietary Astragalus polysaccharides	0:33	Dietary Astragalus polysaccharides	0:33	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	5	26	dep	%	660:660	arg1	0.20 					655:659	0.20 	655:659	0.20 	655:659	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	9	27	theme	prawn	1640:1644	arg1	immunity					1646:1653	prawn immunity	1640:1653	prawn immunity	1640:1653	Thus, dietary APS positively affected the gut-brain axis by altering the microbiota composition, increasing the fecal SCFA content, and enhancing prawn immunity.
37276896	5	28	dep	APS	662:664	arg1	%					660:660	%	660:660	%	660:660	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	2	29	theme	doses	322:326	arg1	effects					301:307	the effects	297:307	the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii	297:420	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	7	30	theme	increased	1136:1144	arg1	levels					1173:1178	increased Lactobacillus and Bacillus levels	1136:1178	increased Lactobacillus and Bacillus levels	1136:1178	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	8	31	theme	enhanced	1244:1251	arg1	function					1260:1267	a significantly enhanced immune function	1228:1267	a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity)	1228:1401	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	0	32	theme	Dietary	0:6	arg1	polysaccharides					19:33	Dietary Astragalus polysaccharides	0:33	Dietary Astragalus polysaccharides	0:33	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	6	33	theme	control	943:949	arg1	group					951:955	the control group	939:955	the control group	939:955	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	5	34	from	activities	701:710	arg1	tissues					726:732	intestinal tissues	715:732	intestinal tissues	715:732	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	2	35	theme	different	312:320	arg1	doses					322:326	different doses	312:326	different doses of APS	312:333	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	5	36	theme	Growth	598:603	arg1	performance					605:615	Growth performance	598:615	Growth performance	598:615	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	7	37	theme	acid	1035:1038	arg1	contents					1040:1047	γ-aminobutyric acid contents	1020:1047	γ-aminobutyric acid contents	1020:1047	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	9	38	theme	microbiota	1567:1576	arg1	composition					1578:1588	the microbiota composition	1563:1588	the microbiota composition	1563:1588	Thus, dietary APS positively affected the gut-brain axis by altering the microbiota composition, increasing the fecal SCFA content, and enhancing prawn immunity.
37276896	2	39	theme	rosenbergii	410:420	arg1	response					364:371	innate immune response	350:371	innate immune response	350:371	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	2	39	theme	rosenbergii	410:420	arg1	axis					388:391	brain-gut axis	378:391	brain-gut axis	378:391	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	2	39	theme	rosenbergii	410:420	arg1	growth					342:347	growth	342:347	growth	342:347	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	7	40	theme	fecal	962:966	arg1	SCFA					992:995	SCFA	992:995	SCFA	992:995	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	7	40	theme	fecal	962:966	arg1	acid					986:989	The fecal short chain fatty acid	958:989	The fecal short chain fatty acid (SCFA)	958:996	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	5	41	from	lipase	681:686	arg1	tissues					726:732	intestinal tissues	715:732	intestinal tissues	715:732	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	6	42	theme	different	876:884	arg1	structure					906:914	a markedly different microbial community structure	865:914	a markedly different microbial community structure	865:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	8	43	theme	immune	1253:1258	arg1	function					1260:1267	a significantly enhanced immune function	1228:1267	a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity)	1228:1401	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	8	44	theme	lysozyme	1384:1391	arg1	activity					1393:1400	lysozyme activity	1384:1400	lysozyme activity	1384:1400	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	4	45	from	%	576:576	arg1	diets					591:595	practical diets	581:595	practical diets	581:595	APS were added at concentration of 0 (control), 0.05, 0.10, 0.15, and 0.20 % in practical diets.
37276896	3	46	theme	molecular	427:435	arg1	weight					437:442	The molecular weight	423:442	The molecular weight	423:442	The molecular weight and the monosaccharide composition of APS were analyzed.
37276896	6	47	dep	bacteria	829:836	arg1	Aeromonas					850:858	Aeromonas	850:858	Aeromonas	850:858	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	47	dep	bacteria	829:836	arg1	Vibrio					839:844	Vibrio	839:844	Vibrio	839:844	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	7	48	theme	γ-aminobutyric	1020:1033	arg1	contents					1040:1047	γ-aminobutyric acid contents	1020:1047	γ-aminobutyric acid contents	1020:1047	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	6	49	theme	bacteria	829:836	arg1	bacteria					829:836	pathogenic intestinal bacteria	807:836	pathogenic intestinal bacteria (Vibrio and Aeromonas)	807:859	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	49	theme	bacteria	829:836	arg1	amounts					796:802	significantly lower amounts	776:802	significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure	776:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	6	49	theme	bacteria	829:836	arg1	structure					906:914	a markedly different microbial community structure	865:914	a markedly different microbial community structure	865:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	7	50	theme	fatty	980:984	arg1	SCFA					992:995	SCFA	992:995	SCFA	992:995	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	7	50	theme	fatty	980:984	arg1	acid					986:989	The fecal short chain fatty acid	958:989	The fecal short chain fatty acid (SCFA)	958:996	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	1	51	theme	beneficial	226:235	arg1	effects					237:243	beneficial effects	226:243	beneficial effects	226:243	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	0	52	theme	innate	58:63	arg1	response					72:79	innate immune response	58:79	innate immune response	58:79	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	8	53	theme	agglutination	1359:1371	arg1	titer					1373:1377	serum agglutination titer	1353:1377	serum agglutination titer	1353:1377	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	6	54	theme	intestinal	818:827	arg1	bacteria					829:836	pathogenic intestinal bacteria	807:836	pathogenic intestinal bacteria (Vibrio and Aeromonas)	807:859	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	8	55	theme	serum	1353:1357	arg1	titer					1373:1377	serum agglutination titer	1353:1377	serum agglutination titer	1353:1377	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	8	56	theme	total	1278:1282	arg1	count					1293:1297	total hemocyte count	1278:1297	total hemocyte count	1278:1297	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	8	57	theme	APS	1198:1200	arg1	supplementation					1202:1216	APS supplementation	1198:1216	APS supplementation	1198:1216	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	4	58	theme	0	536:536	arg1	concentration					519:531	concentration	519:531	concentration of 0 (control), 0.05, 0.10, 0.15, and 0.20 % in practical diets	519:595	APS were added at concentration of 0 (control), 0.05, 0.10, 0.15, and 0.20 % in practical diets.
37276896	0	59	theme	brain-gut	152:160	arg1	axis					162:165	the brain-gut axis	148:165	the brain-gut axis	148:165	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	6	60	theme	pathogenic	807:816	arg1	bacteria					829:836	pathogenic intestinal bacteria	807:836	pathogenic intestinal bacteria (Vibrio and Aeromonas)	807:859	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	9	61	theme	fecal	1606:1610	arg1	content					1617:1623	the fecal SCFA content	1602:1623	the fecal SCFA content	1602:1623	Thus, dietary APS positively affected the gut-brain axis by altering the microbiota composition, increasing the fecal SCFA content, and enhancing prawn immunity.
37276896	7	62	theme	short	968:972	arg1	SCFA					992:995	SCFA	992:995	SCFA	992:995	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	7	62	theme	short	968:972	arg1	acid					986:989	The fecal short chain fatty acid	958:989	The fecal short chain fatty acid (SCFA)	958:996	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	9	63	theme	gut-brain	1536:1544	arg1	axis					1546:1549	the gut-brain axis	1532:1549	the gut-brain axis	1532:1549	Thus, dietary APS positively affected the gut-brain axis by altering the microbiota composition, increasing the fecal SCFA content, and enhancing prawn immunity.
37276896	6	64	theme	community	896:904	arg1	structure					906:914	a markedly different microbial community structure	865:914	a markedly different microbial community structure	865:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	1	65	contain	has	222:224	arg2	effects					237:243	beneficial effects	226:243	beneficial effects	226:243	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	1	65	contain	has	222:224	arg1	Supplementation					168:182	Supplementation	168:182	Supplementation with Astragalus polysaccharides (APS)	168:220	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	0	66	dep	improve	35:41	arg1	Insights					134:141	Insights	134:141	Insights from the brain-gut axis	134:165	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	7	67	theme	chain	974:978	arg1	SCFA					992:995	SCFA	992:995	SCFA	992:995	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	7	67	theme	chain	974:978	arg1	acid					986:989	The fecal short chain fatty acid	958:989	The fecal short chain fatty acid (SCFA)	958:996	The fecal short chain fatty acid (SCFA) and neurotransmitters γ-aminobutyric acid contents increased in the brains of prawns receiving APS, which was potentially associated with increased Lactobacillus and Bacillus levels.
37276896	2	68	theme	APS	331:333	arg1	doses					322:326	different doses	312:326	different doses of APS	312:333	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	8	69	theme	control	1479:1485	arg1	group					1487:1491	the control group	1475:1491	the control group	1475:1491	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	8	70	theme	total	1300:1304	arg1	concentration					1314:1326	total protein concentration	1300:1326	total protein concentration	1300:1326	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	3	71	theme	APS	482:484	arg1	weight					437:442	The molecular weight	423:442	The molecular weight	423:442	The molecular weight and the monosaccharide composition of APS were analyzed.
37276896	3	71	theme	APS	482:484	arg1	composition					467:477	the monosaccharide composition	448:477	the monosaccharide composition of APS	448:484	The molecular weight and the monosaccharide composition of APS were analyzed.
37276896	5	72	theme	protease	692:699	arg1	activities					701:710	protease activities	692:710	protease activities	692:710	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
37276896	4	73	theme	practical	581:589	arg1	diets					591:595	practical diets	581:595	practical diets	581:595	APS were added at concentration of 0 (control), 0.05, 0.10, 0.15, and 0.20 % in practical diets.
37276896	3	74	theme	monosaccharide	452:465	arg1	composition					467:477	the monosaccharide composition	448:477	the monosaccharide composition of APS	448:484	The molecular weight and the monosaccharide composition of APS were analyzed.
37276896	2	75	theme	immune	357:362	arg1	response					364:371	innate immune response	350:371	innate immune response	350:371	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	8	76	theme	hemocyte	1284:1291	arg1	count					1293:1297	total hemocyte count	1278:1297	total hemocyte count	1278:1297	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	1	77	theme	aquatic	248:254	arg1	animals					256:262	aquatic animals	248:262	aquatic animals	248:262	Supplementation with Astragalus polysaccharides (APS) has beneficial effects on aquatic animals.
37276896	0	78	theme	immune	65:70	arg1	response					72:79	innate immune response	58:79	innate immune response	58:79	Dietary Astragalus polysaccharides improve the growth and innate immune response of giant freshwater prawn Macrobrachium rosenbergii: Insights from the brain-gut axis.
37276896	6	79	theme	microbial	886:894	arg1	structure					906:914	a markedly different microbial community structure	865:914	a markedly different microbial community structure	865:914	Prawns receiving APS supplementation had significantly lower amounts of pathogenic intestinal bacteria (Vibrio and Aeromonas) and a markedly different microbial community structure compared with those of the control group.
37276896	8	80	theme	disease	1416:1422	arg1	resistance					1424:1433	improved disease resistance	1407:1433	improved disease resistance to Vibrio anguillarum	1407:1455	Prawns receiving APS supplementation displayed a significantly enhanced immune function (such as total hemocyte count, total protein concentration, phenoloxidase activity, serum agglutination titer, and lysozyme activity) and improved disease resistance to Vibrio anguillarum compared those in the control group.
37276896	2	81	theme	innate	350:355	arg1	response					364:371	innate immune response	350:371	innate immune response	350:371	Herein, we aimed to investigate the effects of different doses of APS on the growth, innate immune response, and brain-gut axis of Macrobrachium rosenbergii.
37276896	5	82	theme	enhanced	672:679	arg1	lipase					681:686	enhanced lipase	672:686	enhanced lipase	672:686	Growth performance increased significantly under 0.05 to 0.20 % APS, with enhanced lipase and protease activities in intestinal tissues.
36300519	0	0	theme	hot	79:81	arg1	water					83:87	hot water	79:87	hot water	79:87	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	0	1	from	composition	10:20	arg1	eggplant					55:62	eggplant	55:62	eggplant	55:62	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	5	2	theme	polysaccharides	1056:1070	arg1	disintegration					1034:1047	the disintegration	1030:1047	the disintegration of the polysaccharides	1030:1070	The results suggest that 1-MCP (5 and 10 μL L-1) and CaCl2 (1, 2, and 3%) could prevent eggplant softening by inhibiting hydrolase enzymes and reducing the disintegration of the polysaccharides.
36300519	4	3	theme	CaCl2	753:757	arg1	treatments					759:768	1-MCP and CaCl2 treatments	743:768	1-MCP and CaCl2 treatments	743:768	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	1	4	theme	postharvest	144:154	arg1	applications					156:167	postharvest applications	144:167	postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%)	144:260	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	5	theme	55°C	201:204	arg1	applications					156:167	postharvest applications	144:167	postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%)	144:260	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	6	6	from	addition	1076:1083	arg1	effective					1112:1120	effective	1112:1120	effective	1112:1120	In addition, 1-MCP and CaCl2 were more effective than hot water treatment in extending postharvest storage life.
36300519	1	7	theme	cell	438:441	arg1	composition					448:458	cell wall composition	438:458	cell wall composition of eggplant fruit	438:476	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	7	8	theme	polyuronide	1246:1256	arg1	content					1258:1264	polyuronide content	1246:1264	polyuronide content	1246:1264	There is a significantly high correlation between firmness, polyuronide content and cell wall enzyme activity.
36300519	1	9	from	effects	133:139	arg1	composition					448:458	cell wall composition	438:458	cell wall composition of eggplant fruit	438:476	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	9	from	effects	133:139	arg1	β-galactosidase					343:357	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	β-galactosidase	343:357	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	9	from	effects	133:139	arg1	firmness					425:432	the fruit firmness	415:432	the fruit firmness	415:432	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	9	from	effects	133:139	arg1	polygalacturonase					265:281	polygalacturonase	265:281	polygalacturonase (PG)	265:286	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	9	from	effects	133:139	arg1	methylesterase					296:309	pectin methylesterase	289:309	pectin methylesterase (PME)	289:315	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	9	from	effects	133:139	arg1	PG					284:285	PG	284:285	PG	284:285	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	9	from	effects	133:139	arg1	PME					312:314	PME	312:314	PME	312:314	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	10	theme	β-Gal	360:364	arg1	β-galactosidase					343:357	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	β-galactosidase	343:357	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	11	theme	applications	156:167	arg1	effects					133:139	The effects	129:139	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit	129:476	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	0	12	from	activities	41:50	arg1	eggplant					55:62	eggplant	55:62	eggplant	55:62	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	1	13	theme	pectin	289:294	arg1	methylesterase					296:309	pectin methylesterase	289:309	pectin methylesterase (PME)	289:315	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	13	theme	pectin	289:294	arg1	PME					312:314	PME	312:314	PME	312:314	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	4	14	theme	water	804:808	arg1	depolymerization					784:799	the depolymerization	780:799	the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides	780:875	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	0	15	theme	wall	5:8	arg1	composition					10:20	Cell wall composition	0:20	Cell wall composition	0:20	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	2	16	theme	eggplants	541:549	arg1	firmness					551:558	the eggplants firmness	537:558	the eggplants firmness	537:558	The results showed that the decrease in the eggplants firmness was delayed by HWT, 1-MCP, and CaCl2 treatments during storage compared with the control.
36300519	1	17	theme	hot	172:174	arg1	HWT					183:185	hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%)	172:260	HWT	183:185	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	0	18	theme	Cell	0:3	arg1	composition					10:20	Cell wall composition	0:20	Cell wall composition	0:20	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	1	19	theme	1-MCP	208:212	arg1	applications					156:167	postharvest applications	144:167	postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%)	144:260	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	4	20	dep	water	804:808	arg1	polyuronides					864:875	soluble polyuronides	856:875	soluble polyuronides	856:875	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	1	21	theme	β-1,4-glucanase	371:385	arg1	activities					399:408	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	activities	399:408	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	22	theme	water	176:180	arg1	HWT					183:185	hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%)	172:260	HWT	183:185	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	0	23	theme	chloride	108:115	arg1	treatments					117:126	calcium chloride treatments	100:126	calcium chloride treatments	100:126	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	4	24	theme	carbonate	840:848	arg1	depolymerization					784:799	the depolymerization	780:799	the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides	780:875	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	2	25	from	decrease	525:532	arg1	firmness					551:558	the eggplants firmness	537:558	the eggplants firmness	537:558	The results showed that the decrease in the eggplants firmness was delayed by HWT, 1-MCP, and CaCl2 treatments during storage compared with the control.
36300519	1	26	theme	eggplant	463:470	arg1	fruit					472:476	eggplant fruit	463:476	eggplant fruit	463:476	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	0	27	theme	calcium	100:106	arg1	treatments					117:126	calcium chloride treatments	100:126	calcium chloride treatments	100:126	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	6	28	from	effective	1112:1120	arg1	addition					1076:1083	addition	1076:1083	addition	1076:1083	In addition, 1-MCP and CaCl2 were more effective than hot water treatment in extending postharvest storage life.
36300519	1	29	theme	β-1,4-Glu	388:396	arg1	activities					399:408	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	activities	399:408	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	30	theme	HWT	183:185	arg1	applications					156:167	postharvest applications	144:167	postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%)	144:260	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	composition					448:458	cell wall composition	438:458	cell wall composition of eggplant fruit	438:476	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	β-galactosidase					343:357	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	β-galactosidase	343:357	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	firmness					425:432	the fruit firmness	415:432	the fruit firmness	415:432	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	polygalacturonase					265:281	polygalacturonase	265:281	polygalacturonase (PG)	265:286	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	methylesterase					296:309	pectin methylesterase	289:309	pectin methylesterase (PME)	289:315	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	activities					399:408	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	activities	399:408	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	PG					284:285	PG	284:285	PG	284:285	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	31	theme	fruit	472:476	arg1	PME					312:314	PME	312:314	PME	312:314	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	4	32	theme	soluble	856:862	arg1	polyuronides					864:875	soluble polyuronides	856:875	soluble polyuronides	856:875	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	6	33	theme	hot	1127:1129	arg1	treatment					1137:1145	hot water treatment	1127:1145	hot water treatment	1127:1145	In addition, 1-MCP and CaCl2 were more effective than hot water treatment in extending postharvest storage life.
36300519	5	34	theme	hydrolase	999:1007	arg1	enzymes					1009:1015	hydrolase enzymes	999:1015	hydrolase enzymes	999:1015	The results suggest that 1-MCP (5 and 10 μL L-1) and CaCl2 (1, 2, and 3%) could prevent eggplant softening by inhibiting hydrolase enzymes and reducing the disintegration of the polysaccharides.
36300519	0	35	theme	enzyme-related	26:39	arg1	activities					41:50	enzyme-related activities	26:50	enzyme-related activities in eggplant	26:62	Cell wall composition and enzyme-related activities in eggplant as affected by hot water, 1-MCP and calcium chloride treatments.
36300519	7	36	theme	high	1211:1214	arg1	correlation					1216:1226	a significantly high correlation	1195:1226	a significantly high correlation between firmness, polyuronide content and cell wall enzyme activity	1195:1294	There is a significantly high correlation between firmness, polyuronide content and cell wall enzyme activity.
36300519	2	37	dep	HWT	575:577	arg1	treatments					597:606	treatments	597:606	treatments	597:606	The results showed that the decrease in the eggplants firmness was delayed by HWT, 1-MCP, and CaCl2 treatments during storage compared with the control.
36300519	6	38	theme	postharvest	1160:1170	arg1	life					1180:1183	postharvest storage life	1160:1183	postharvest storage life	1160:1183	In addition, 1-MCP and CaCl2 were more effective than hot water treatment in extending postharvest storage life.
36300519	1	39	theme	α-galactosidase	318:332	arg1	β-galactosidase					343:357	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	β-galactosidase	343:357	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	3	40	dep	1-MCP	693:697	arg1	the					689:691	the	689:691	the	689:691	However, HWTs were less effective than the 1-MCP and CaCl2 treatments.
36300519	3	40	dep	1-MCP	693:697	arg1	treatments					709:718	treatments	709:718	treatments	709:718	However, HWTs were less effective than the 1-MCP and CaCl2 treatments.
36300519	7	41	theme	enzyme	1280:1285	arg1	activity					1287:1294	enzyme activity	1280:1294	enzyme activity	1280:1294	There is a significantly high correlation between firmness, polyuronide content and cell wall enzyme activity.
36300519	1	42	theme	α-Gal	335:339	arg1	β-galactosidase					343:357	α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities	318:408	β-galactosidase	343:357	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	4	43	theme	sodium	833:838	arg1	SSP					851:853	SSP	851:853	SSP	851:853	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	4	43	theme	sodium	833:838	arg1	carbonate					840:848	sodium carbonate	833:848	sodium carbonate (SSP)	833:854	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	6	44	theme	water	1131:1135	arg1	treatment					1137:1145	hot water treatment	1127:1145	hot water treatment	1127:1145	In addition, 1-MCP and CaCl2 were more effective than hot water treatment in extending postharvest storage life.
36300519	4	45	theme	CDTA	817:820	arg1	depolymerization					784:799	the depolymerization	780:799	the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides	780:875	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	6	46	theme	storage	1172:1178	arg1	life					1180:1183	postharvest storage life	1160:1183	postharvest storage life	1160:1183	In addition, 1-MCP and CaCl2 were more effective than hot water treatment in extending postharvest storage life.
36300519	5	47	theme	eggplant	966:973	arg1	softening					975:983	eggplant softening	966:983	eggplant softening	966:983	The results suggest that 1-MCP (5 and 10 μL L-1) and CaCl2 (1, 2, and 3%) could prevent eggplant softening by inhibiting hydrolase enzymes and reducing the disintegration of the polysaccharides.
36300519	7	48	theme	cell	1270:1273	arg1	wall					1275:1278	cell wall	1270:1278	cell wall	1270:1278	There is a significantly high correlation between firmness, polyuronide content and cell wall enzyme activity.
36300519	4	49	theme	1-MCP	743:747	arg1	treatments					759:768	1-MCP and CaCl2 treatments	743:768	1-MCP and CaCl2 treatments	743:768	The results show that 1-MCP and CaCl2 treatments inhibited the depolymerization of water (WSP), CDTA (CSP), and sodium carbonate (SSP) soluble polyuronides.
36300519	1	50	theme	wall	443:446	arg1	composition					448:458	cell wall composition	438:458	cell wall composition of eggplant fruit	438:476	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	1	51	theme	fruit	419:423	arg1	firmness					425:432	the fruit firmness	415:432	the fruit firmness	415:432	The effects of postharvest applications of hot water (HWT) (45, 50, and 55°C), 1-MCP (1, 5, and 10 μL L-1), and CaCl2 (1, 2, and 3%) on polygalacturonase (PG), pectin methylesterase (PME), α-galactosidase (α-Gal), β-galactosidase (β-Gal) and β-1,4-glucanase (β-1,4-Glu) activities, and the fruit firmness and cell wall composition of eggplant fruit were investigated.
36300519	7	52	dep	firmness	1236:1243	arg1	activity					1287:1294	enzyme activity	1280:1294	enzyme activity	1280:1294	There is a significantly high correlation between firmness, polyuronide content and cell wall enzyme activity.
36845055	10	0	theme	HIF-1	1217:1221	arg1	binding					1352:1358	carbohydrate binding	1339:1358	carbohydrate binding	1339:1358	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	0	theme	HIF-1	1217:1221	arg1	GO					1405:1406	GO	1405:1406	GO	1405:1406	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	0	theme	HIF-1	1217:1221	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	0	theme	HIF-1	1217:1221	arg1	binding					1330:1336	growth factor receptor binding	1307:1336	growth factor receptor binding	1307:1336	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	0	theme	HIF-1	1217:1221	arg1	activity					1386:1393	protein homodimerization activity	1361:1393	protein homodimerization activity	1361:1393	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	0	theme	HIF-1	1217:1221	arg1	metabolism					1252:1261	Galactose metabolism	1242:1261	Galactose metabolism	1242:1261	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	0	theme	HIF-1	1217:1221	arg1	resistance					1295:1304	EGFR tyrosine kinase inhibitor resistance	1264:1304	EGFR tyrosine kinase inhibitor resistance	1264:1304	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	1	1	from	polysaccharides	177:191	arg1	treatment					215:223	treatment	215:223	treatment	215:223	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	1	1	from	polysaccharides	177:191	arg1	prevention					200:209	prevention	200:209	prevention	200:209	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	11	2	theme	seabuckthorn	1548:1559	arg1	polysaccharides					1561:1575	seabuckthorn polysaccharides	1548:1575	seabuckthorn polysaccharides	1548:1575	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	4	3	theme	active	590:595	arg1	ingredients					597:607	active ingredients	590:607	active ingredients	590:607	The correlation score was greater than 5 targets for 2727; 15 intersection targets of active ingredients and disease were obtained by Venn diagram.
36845055	10	4	theme	protein	1361:1367	arg1	activity					1386:1393	protein homodimerization activity	1361:1393	protein homodimerization activity	1361:1393	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	4	theme	protein	1361:1367	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	8	5	theme	molecular	1057:1065	arg1	docking					1067:1073	molecular docking	1057:1073	molecular docking	1057:1073	SailVina and PyMOL software were used for molecular docking to verify binding strength.
36845055	1	6	from	target	154:159	arg1	treatment					215:223	treatment	215:223	treatment	215:223	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	1	6	from	target	154:159	arg1	prevention					200:209	prevention	200:209	prevention	200:209	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	6	7	used	used	828:831	arg2	software					815:822	Cytoscape3.6.0 software	800:822	Cytoscape3.6.0 software	800:822	Cytoscape3.6.0 software was used for visualization and network topology analysis to obtain core targets.
36845055	10	8	theme	Galactose	1242:1250	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	8	theme	Galactose	1242:1250	arg1	metabolism					1252:1261	Galactose metabolism	1242:1261	Galactose metabolism	1242:1261	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	0	9	theme	cancer	101:106	arg1	treatment					79:87	treatment	79:87	treatment	79:87	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	0	9	theme	cancer	101:106	arg1	prevention					64:73	prevention	64:73	prevention	64:73	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	11	10	theme	cervical	1612:1619	arg1	cancer					1621:1626	cervical cancer	1612:1626	cervical cancer	1612:1626	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	8	11	theme	PyMOL	1028:1032	arg1	software					1034:1041	PyMOL software	1028:1041	PyMOL software	1028:1041	SailVina and PyMOL software were used for molecular docking to verify binding strength.
36845055	10	12	theme	kinase	1278:1283	arg1	inhibitor					1285:1293	EGFR tyrosine kinase inhibitor	1264:1293	EGFR tyrosine kinase inhibitor resistance	1264:1304	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	13	theme	homodimerization	1369:1384	arg1	activity					1386:1393	protein homodimerization activity	1361:1393	protein homodimerization activity	1361:1393	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	13	theme	homodimerization	1369:1384	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	0	14	theme	cervical	92:99	arg1	cancer					101:106	cervical cancer	92:106	cervical cancer	92:106	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	6	15	theme	topology	863:870	arg1	analysis					872:879	network topology analysis	855:879	network topology analysis	855:879	Cytoscape3.6.0 software was used for visualization and network topology analysis to obtain core targets.
36845055	11	16	from	effect	1538:1543	arg1	treatment					1599:1607	treatment	1599:1607	treatment	1599:1607	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	11	16	from	effect	1538:1543	arg1	prevention					1584:1593	prevention	1584:1593	prevention	1584:1593	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	1	17	theme	multi-target	264:275	arg1	mechanism					295:303	its multi-target and multi-pathway mechanism	260:303	its multi-target and multi-pathway mechanism	260:303	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	0	18	theme	mechanism	112:120	arg1	study					122:126	mechanism study	112:126	mechanism study	112:126	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	11	19	theme	further	1733:1739	arg1	research					1741:1748	further research	1733:1748	further research on the activity of seabuckthorn polysaccharides	1733:1796	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	4	20	theme	Venn	638:641	arg1	diagram					643:649	Venn diagram	638:649	Venn diagram	638:649	The correlation score was greater than 5 targets for 2727; 15 intersection targets of active ingredients and disease were obtained by Venn diagram.
36845055	7	21	dep	Ontology	961:968	arg1	GO					971:972	GO	971:972	GO	971:972	Kyoto encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) were analyzed using Metascape database.
36845055	3	22	theme	cancer	438:443	arg1	targets					453:459	Cervical cancer related targets	429:459	Cervical cancer related targets	429:459	Cervical cancer related targets were obtained from the GeneCards database.
36845055	1	23	theme	Seabuckthorn	164:175	arg1	polysaccharides					177:191	Seabuckthorn polysaccharides	164:191	Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer	164:242	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	4	24	theme	ingredients	597:607	arg1	targets					579:585	15 intersection targets	563:585	15 intersection targets of active ingredients and disease	563:619	The correlation score was greater than 5 targets for 2727; 15 intersection targets of active ingredients and disease were obtained by Venn diagram.
36845055	4	25	theme	correlation	508:518	arg1	targets					545:551	greater than 5 targets	530:551	greater than 5 targets for 2727	530:560	The correlation score was greater than 5 targets for 2727; 15 intersection targets of active ingredients and disease were obtained by Venn diagram.
36845055	4	25	theme	correlation	508:518	arg1	score					520:524	The correlation score	504:524	The correlation score	504:524	The correlation score was greater than 5 targets for 2727; 15 intersection targets of active ingredients and disease were obtained by Venn diagram.
36845055	8	26	theme	binding	1085:1091	arg1	strength					1093:1100	binding strength	1085:1100	binding strength	1085:1100	SailVina and PyMOL software were used for molecular docking to verify binding strength.
36845055	4	27	theme	disease	613:619	arg1	targets					579:585	15 intersection targets	563:585	15 intersection targets of active ingredients and disease	563:619	The correlation score was greater than 5 targets for 2727; 15 intersection targets of active ingredients and disease were obtained by Venn diagram.
36845055	10	28	theme	receptor	1321:1328	arg1	binding					1330:1336	growth factor receptor binding	1307:1336	growth factor receptor binding	1307:1336	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	28	theme	receptor	1321:1328	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	1	29	theme	polysaccharides	177:191	arg1	target					154:159	the target	150:159	the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer	150:242	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	9	30	dep	Results	1103:1109	arg1	total					1113:1117	A total	1111:1117	Results A total of 15 core targets	1103:1136	Results A total of 15 core targets were obtained for cervical cancer.
36845055	2	31	theme	Swisstarget	324:334	arg1	database					336:343	the Swisstarget database	320:343	the Swisstarget database	320:343	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	2	31	theme	Swisstarget	324:334	arg1	total					348:352	a total	346:352	a total of 61 potential targets of polysaccharide active components	346:412	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	10	32	theme	factor	1314:1319	arg1	receptor					1321:1328	growth factor receptor	1307:1328	growth factor receptor binding	1307:1336	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	2	33	theme	polysaccharide	381:394	arg1	components					403:412	polysaccharide active components	381:412	polysaccharide active components	381:412	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	11	34	theme	seabuckthorn	1769:1780	arg1	polysaccharides					1782:1796	seabuckthorn polysaccharides	1769:1796	seabuckthorn polysaccharides	1769:1796	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	8	35	used	used	1048:1051	arg2	software					1034:1041	PyMOL software	1028:1041	PyMOL software	1028:1041	SailVina and PyMOL software were used for molecular docking to verify binding strength.
36845055	8	35	used	used	1048:1051	arg2	SailVina					1015:1022	SailVina	1015:1022	SailVina	1015:1022	SailVina and PyMOL software were used for molecular docking to verify binding strength.
36845055	3	36	theme	related	445:451	arg1	targets					453:459	Cervical cancer related targets	429:459	Cervical cancer related targets	429:459	Cervical cancer related targets were obtained from the GeneCards database.
36845055	0	37	theme	marker	21:26	arg1	Prediction					0:9	Prediction	0:9	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.	0:127	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	1	38	theme	multi-pathway	281:293	arg1	mechanism					295:303	its multi-target and multi-pathway mechanism	260:303	its multi-target and multi-pathway mechanism	260:303	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	6	39	theme	network	855:861	arg1	analysis					872:879	network topology analysis	855:879	network topology analysis	855:879	Cytoscape3.6.0 software was used for visualization and network topology analysis to obtain core targets.
36845055	7	40	theme	Metascape	995:1003	arg1	database					1005:1012	Metascape database	995:1012	Metascape database	995:1012	Kyoto encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) were analyzed using Metascape database.
36845055	0	41	theme	active	14:19	arg1	marker					21:26	active marker	14:26	active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study	14:126	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	11	42	dep	Conclusion	1523:1532	arg1	characterized					1631:1643	characterized	1631:1643	is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides	1628:1796	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	0	43	theme	study	122:126	arg1	treatment					79:87	treatment	79:87	treatment	79:87	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	0	43	theme	study	122:126	arg1	prevention					64:73	prevention	64:73	prevention	64:73	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	3	44	theme	GeneCards	484:492	arg1	database					494:501	the GeneCards database	480:501	the GeneCards database	480:501	Cervical cancer related targets were obtained from the GeneCards database.
36845055	0	45	theme	seabuckthorn	31:42	arg1	polysaccharides					44:58	seabuckthorn polysaccharides	31:58	seabuckthorn polysaccharides	31:58	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	4	46	theme	intersection	566:577	arg1	targets					579:585	15 intersection targets	563:585	15 intersection targets of active ingredients and disease	563:619	The correlation score was greater than 5 targets for 2727; 15 intersection targets of active ingredients and disease were obtained by Venn diagram.
36845055	5	47	theme	Protein-Protein	756:770	arg1	PPI					794:796	PPI	794:796	PPI	794:796	Cytoscape3.6.0 software was used to construct the Polysaccharide composition-Target-Disease Network and Protein-Protein Interaction Networks (PPI).
36845055	5	47	theme	Protein-Protein	756:770	arg1	Networks					784:791	Protein-Protein Interaction Networks	756:791	Protein-Protein Interaction Networks (PPI)	756:797	Cytoscape3.6.0 software was used to construct the Polysaccharide composition-Target-Disease Network and Protein-Protein Interaction Networks (PPI).
36845055	5	48	theme	Interaction	772:782	arg1	PPI					794:796	PPI	794:796	PPI	794:796	Cytoscape3.6.0 software was used to construct the Polysaccharide composition-Target-Disease Network and Protein-Protein Interaction Networks (PPI).
36845055	5	48	theme	Interaction	772:782	arg1	Networks					784:791	Protein-Protein Interaction Networks	756:791	Protein-Protein Interaction Networks (PPI)	756:797	Cytoscape3.6.0 software was used to construct the Polysaccharide composition-Target-Disease Network and Protein-Protein Interaction Networks (PPI).
36845055	10	49	theme	carbohydrate	1339:1350	arg1	binding					1352:1358	carbohydrate binding	1339:1358	carbohydrate binding	1339:1358	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	49	theme	carbohydrate	1339:1350	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	2	50	theme	components	403:412	arg1	targets					370:376	61 potential targets	357:376	61 potential targets of polysaccharide active components	357:412	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	3	51	theme	Cervical	429:436	arg1	targets					453:459	Cervical cancer related targets	429:459	Cervical cancer related targets	429:459	Cervical cancer related targets were obtained from the GeneCards database.
36845055	10	52	dep	GO	1405:1406	arg1	showed					1444:1449	showed	1444:1449	showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose	1444:1520	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	1	53	from	treatment	215:223	arg1	target					154:159	the target	150:159	the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer	150:242	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	11	54	theme	scientific	1712:1721	arg1	basis					1723:1727	scientific basis	1712:1727	scientific basis for further research on the activity of seabuckthorn polysaccharides	1712:1796	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	11	55	from	research	1741:1748	arg1	activity					1757:1764	the activity	1753:1764	the activity of seabuckthorn polysaccharides	1753:1796	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	9	56	theme	cervical	1156:1163	arg1	cancer					1165:1170	cervical cancer	1156:1170	cervical cancer	1156:1170	Results A total of 15 core targets were obtained for cervical cancer.
36845055	0	57	theme	polysaccharides	44:58	arg1	marker					21:26	active marker	14:26	active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study	14:126	Prediction of active marker of seabuckthorn polysaccharides for prevention and treatment of cervical cancer and mechanism study.
36845055	11	58	dep	prevention	1584:1593	arg1	the					1580:1582	the	1580:1582	the	1580:1582	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	11	59	theme	polysaccharides	1782:1796	arg1	activity					1757:1764	the activity	1753:1764	the activity of seabuckthorn polysaccharides	1753:1796	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	1	60	from	prevention	200:209	arg1	target					154:159	the target	150:159	the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer	150:242	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	7	61	theme	Gene	956:959	arg1	Ontology					961:968	Gene Ontology	956:968	Gene Ontology (GO)	956:973	Kyoto encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) were analyzed using Metascape database.
36845055	5	62	theme	Polysaccharide	702:715	arg1	Network					744:750	the Polysaccharide composition-Target-Disease Network	698:750	the Polysaccharide composition-Target-Disease Network	698:750	Cytoscape3.6.0 software was used to construct the Polysaccharide composition-Target-Disease Network and Protein-Protein Interaction Networks (PPI).
36845055	11	63	theme	polysaccharides	1561:1575	arg1	effect					1538:1543	The effect	1534:1543	The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer	1534:1626	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	9	64	theme	core	1125:1128	arg1	targets					1130:1136	15 core targets	1122:1136	15 core targets	1122:1136	Results A total of 15 core targets were obtained for cervical cancer.
36845055	10	65	theme	Molecular	1426:1434	arg1	docking					1436:1442	Molecular docking	1426:1442	Molecular docking	1426:1442	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	66	theme	tyrosine	1269:1276	arg1	inhibitor					1285:1293	EGFR tyrosine kinase inhibitor	1264:1293	EGFR tyrosine kinase inhibitor resistance	1264:1304	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	1	67	theme	cervical	228:235	arg1	cancer					237:242	cervical cancer	228:242	cervical cancer	228:242	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	5	68	theme	composition-Target-Disease	717:742	arg1	Network					744:750	the Polysaccharide composition-Target-Disease Network	698:750	the Polysaccharide composition-Target-Disease Network	698:750	Cytoscape3.6.0 software was used to construct the Polysaccharide composition-Target-Disease Network and Protein-Protein Interaction Networks (PPI).
36845055	6	69	theme	core	891:894	arg1	targets					896:902	core targets	891:902	core targets	891:902	Cytoscape3.6.0 software was used for visualization and network topology analysis to obtain core targets.
36845055	9	70	theme	targets	1130:1136	arg1	total					1113:1117	A total	1111:1117	Results A total of 15 core targets	1103:1136	Results A total of 15 core targets were obtained for cervical cancer.
36845055	10	71	theme	EGFR	1264:1267	arg1	inhibitor					1285:1293	EGFR tyrosine kinase inhibitor	1264:1293	EGFR tyrosine kinase inhibitor resistance	1264:1304	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	1	72	theme	cancer	237:242	arg1	treatment					215:223	treatment	215:223	treatment	215:223	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	1	72	theme	cancer	237:242	arg1	prevention					200:209	prevention	200:209	prevention	200:209	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	10	73	theme	signaling	1223:1231	arg1	binding					1352:1358	carbohydrate binding	1339:1358	carbohydrate binding	1339:1358	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	73	theme	signaling	1223:1231	arg1	GO					1405:1406	GO	1405:1406	GO	1405:1406	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	73	theme	signaling	1223:1231	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	73	theme	signaling	1223:1231	arg1	binding					1330:1336	growth factor receptor binding	1307:1336	growth factor receptor binding	1307:1336	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	73	theme	signaling	1223:1231	arg1	activity					1386:1393	protein homodimerization activity	1361:1393	protein homodimerization activity	1361:1393	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	73	theme	signaling	1223:1231	arg1	metabolism					1252:1261	Galactose metabolism	1242:1261	Galactose metabolism	1242:1261	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	73	theme	signaling	1223:1231	arg1	resistance					1295:1304	EGFR tyrosine kinase inhibitor resistance	1264:1304	EGFR tyrosine kinase inhibitor resistance	1264:1304	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	74	theme	inhibitor	1285:1293	arg1	resistance					1295:1304	EGFR tyrosine kinase inhibitor resistance	1264:1304	EGFR tyrosine kinase inhibitor resistance	1264:1304	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	10	74	theme	inhibitor	1285:1293	arg1	pathway					1233:1239	HIF-1 signaling pathway	1217:1239	HIF-1 signaling pathway	1217:1239	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	1	75	dep	prevention	200:209	arg1	the					196:198	the	196:198	the	196:198	Objective To predict the target of Seabuckthorn polysaccharides in the prevention and treatment of cervical cancer, and to explore its multi-target and multi-pathway mechanism.
36845055	10	76	theme	KEGG	1412:1415	arg1	entries					1417:1423	KEGG entries	1412:1423	KEGG entries	1412:1423	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	2	77	theme	targets	370:376	arg1	database					336:343	the Swisstarget database	320:343	the Swisstarget database	320:343	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	2	77	theme	targets	370:376	arg1	total					348:352	a total	346:352	a total of 61 potential targets of polysaccharide active components	346:412	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	2	78	theme	active	396:401	arg1	components					403:412	polysaccharide active components	381:412	polysaccharide active components	381:412	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	11	79	theme	cancer	1621:1626	arg1	treatment					1599:1607	treatment	1599:1607	treatment	1599:1607	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	11	79	theme	cancer	1621:1626	arg1	prevention					1584:1593	prevention	1584:1593	prevention	1584:1593	Conclusion The effect of seabuckthorn polysaccharides on the prevention and treatment of cervical cancer is characterized by multi-component, multi-target and multi-pathway, which provides scientific basis for further research on the activity of seabuckthorn polysaccharides.
36845055	2	80	theme	potential	360:368	arg1	targets					370:376	61 potential targets	357:376	61 potential targets of polysaccharide active components	357:412	Methods Using the Swisstarget database, a total of 61 potential targets of polysaccharide active components were obtained.
36845055	10	81	theme	growth	1307:1312	arg1	receptor					1321:1328	growth factor receptor	1307:1328	growth factor receptor binding	1307:1336	These targets are significantly enriched in HIF-1 signaling pathway, Galactose metabolism, EGFR tyrosine kinase inhibitor resistance, growth factor receptor binding, carbohydrate binding, protein homodimerization activity and other GO and KEGG entries; Molecular docking showed that ADA and GLB1 were well bound to Glucose, D-Mannose, and Galactose.
36845055	5	82	used	used	680:683	arg2	software					667:674	Cytoscape3.6.0 software	652:674	Cytoscape3.6.0 software	652:674	Cytoscape3.6.0 software was used to construct the Polysaccharide composition-Target-Disease Network and Protein-Protein Interaction Networks (PPI).
36433110	0	0	from	Synthesis	8:16	arg1	Fiber					55:59	Guar Gum/PVOH Composite Fiber	31:59	Guar Gum/PVOH Composite Fiber	31:59	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	1	1	theme	infections	153:162	arg1	number					112:117	the number	108:117	the number of antibiotic-resistant bacterial infections	108:162	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
36433110	1	2	theme	new	230:232	arg1	treatments					274:283	new alternative bactericidal wound dressing treatments	230:283	new alternative bactericidal wound dressing treatments	230:283	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
36433110	6	3	theme	regrowth	838:845	arg1	step					847:850	a second successful alkaline thermo-chemical regrowth step	793:850	a second successful alkaline thermo-chemical regrowth step	793:850	Thus, a second successful alkaline thermo-chemical regrowth step was added to the process to treat the obtained fibers.
36433110	1	4	theme	alternative	234:244	arg1	treatments					274:283	new alternative bactericidal wound dressing treatments	230:283	new alternative bactericidal wound dressing treatments	230:283	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
36433110	0	5	theme	Bactericidal	80:91	arg1	Release					93:99	Potential Bactericidal Release	70:99	Potential Bactericidal Release	70:99	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	6	6	theme	alkaline	813:820	arg1	step					847:850	a second successful alkaline thermo-chemical regrowth step	793:850	a second successful alkaline thermo-chemical regrowth step	793:850	Thus, a second successful alkaline thermo-chemical regrowth step was added to the process to treat the obtained fibers.
36433110	3	7	theme	zinc	540:543	arg1	nanoparticles					551:563	aluminum-doped zinc oxide nanoparticles	525:563	aluminum-doped zinc oxide nanoparticles	525:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	1	8	theme	bactericidal	246:257	arg1	treatments					274:283	new alternative bactericidal wound dressing treatments	230:283	new alternative bactericidal wound dressing treatments	230:283	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
36433110	0	9	theme	Potential	70:78	arg1	Release					93:99	Potential Bactericidal Release	70:99	Potential Bactericidal Release	70:99	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	3	10	theme	oxide	545:549	arg1	nanoparticles					551:563	aluminum-doped zinc oxide nanoparticles	525:563	aluminum-doped zinc oxide nanoparticles	525:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	2	11	from	use	331:333	arg1	technologies					401:412	innovative biopolymer technologies	379:412	innovative biopolymer technologies	379:412	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	2	11	from	use	331:333	arg1	seeds					370:374	seeds	370:374	seeds	370:374	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	2	11	from	use	331:333	arg1	plants					359:364	plants	359:364	plants	359:364	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	2	12	theme	polysaccharides	338:352	arg1	use					331:333	the use	327:333	the use of polysaccharides from plants and seeds in innovative biopolymer technologies	327:412	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	1	13	theme	wound	259:263	arg1	treatments					274:283	new alternative bactericidal wound dressing treatments	230:283	new alternative bactericidal wound dressing treatments	230:283	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
36433110	6	14	theme	successful	802:811	arg1	step					847:850	a second successful alkaline thermo-chemical regrowth step	793:850	a second successful alkaline thermo-chemical regrowth step	793:850	Thus, a second successful alkaline thermo-chemical regrowth step was added to the process to treat the obtained fibers.
36433110	2	15	theme	innovative	379:388	arg1	technologies					401:412	innovative biopolymer technologies	379:412	innovative biopolymer technologies	379:412	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	4	16	theme	Citric	600:605	arg1	acid					607:610	Citric acid	600:610	Citric acid	600:610	Citric acid was added to the mixture to increase spinnability.
36433110	6	17	theme	obtained	890:897	arg1	fibers					899:904	the obtained fibers	886:904	the obtained fibers	886:904	Thus, a second successful alkaline thermo-chemical regrowth step was added to the process to treat the obtained fibers.
36433110	6	18	theme	second	795:800	arg1	step					847:850	a second successful alkaline thermo-chemical regrowth step	793:850	a second successful alkaline thermo-chemical regrowth step	793:850	Thus, a second successful alkaline thermo-chemical regrowth step was added to the process to treat the obtained fibers.
36433110	3	19	theme	aluminum-doped	525:538	arg1	nanoparticles					551:563	aluminum-doped zinc oxide nanoparticles	525:563	aluminum-doped zinc oxide nanoparticles	525:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	8	20	theme	thermo-chemical	1125:1139	arg1	treatment					1141:1149	the alkaline thermo-chemical treatment	1112:1149	the alkaline thermo-chemical treatment	1112:1149	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	8	21	theme	antibacterial	1045:1057	arg1	results					1065:1071	The Staphylococcus aureus antibacterial assay results	1019:1071	The Staphylococcus aureus antibacterial assay results	1019:1071	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	0	22	theme	In	0:1	arg1	Synthesis					8:16	In Situ Synthesis	0:16	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber	0:59	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	1	23	theme	dressing	265:272	arg1	treatments					274:283	new alternative bactericidal wound dressing treatments	230:283	new alternative bactericidal wound dressing treatments	230:283	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
36433110	8	24	theme	alkaline	1116:1123	arg1	treatment					1141:1149	the alkaline thermo-chemical treatment	1112:1149	the alkaline thermo-chemical treatment	1112:1149	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	2	25	from	plants	359:364	arg1	polysaccharides					338:352	polysaccharides	338:352	polysaccharides from plants and seeds	338:374	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	2	25	from	plants	359:364	arg1	use					331:333	the use	327:333	the use of polysaccharides from plants and seeds in innovative biopolymer technologies	327:412	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	5	26	theme	zinc	693:696	arg1	nanoparticles					704:716	zinc oxide nanoparticles	693:716	zinc oxide nanoparticles	693:716	However, depending on the pH, zinc oxide nanoparticles are partially dissociated, decreasing their bactericidal efficiency.
36433110	7	27	theme	bactericidal	994:1005	arg1	properties					1007:1016	their bactericidal properties	988:1016	their bactericidal properties	988:1016	This alkaline thermo-chemical treatment reconstituted both the nanoparticles and their bactericidal properties.
36433110	2	28	theme	key	420:422	arg1	importance					424:433	key importance	420:433	key importance	420:433	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	5	29	theme	oxide	698:702	arg1	nanoparticles					704:716	zinc oxide nanoparticles	693:716	zinc oxide nanoparticles	693:716	However, depending on the pH, zinc oxide nanoparticles are partially dissociated, decreasing their bactericidal efficiency.
36433110	3	30	theme	bio-nano-composite	450:467	arg1	membranes					503:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes	450:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles	450:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	3	31	theme	PVOH	497:500	arg1	membranes					503:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes	450:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles	450:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	0	32	theme	AZO-Np	21:26	arg1	Synthesis					8:16	In Situ Synthesis	0:16	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber	0:59	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	8	33	theme	aureus	1038:1043	arg1	results					1065:1071	The Staphylococcus aureus antibacterial assay results	1019:1071	The Staphylococcus aureus antibacterial assay results	1019:1071	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	2	34	from	seeds	370:374	arg1	polysaccharides					338:352	polysaccharides	338:352	polysaccharides from plants and seeds	338:374	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	2	34	from	seeds	370:374	arg1	use					331:333	the use	327:333	the use of polysaccharides from plants and seeds in innovative biopolymer technologies	327:412	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	2	35	theme	biopolymer	390:399	arg1	technologies					401:412	innovative biopolymer technologies	379:412	innovative biopolymer technologies	379:412	Within this context, the use of polysaccharides from plants and seeds in innovative biopolymer technologies is of key importance.
36433110	0	36	theme	Gum/PVOH	36:43	arg1	Fiber					55:59	Guar Gum/PVOH Composite Fiber	31:59	Guar Gum/PVOH Composite Fiber	31:59	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	5	37	theme	bactericidal	762:773	arg1	efficiency					775:784	their bactericidal efficiency	756:784	their bactericidal efficiency	756:784	However, depending on the pH, zinc oxide nanoparticles are partially dissociated, decreasing their bactericidal efficiency.
36433110	3	38	theme	alcohol	488:494	arg1	membranes					503:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes	450:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles	450:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	0	39	theme	Guar	31:34	arg1	Fiber					55:59	Guar Gum/PVOH Composite Fiber	31:59	Guar Gum/PVOH Composite Fiber	31:59	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	8	40	theme	%	1165:1165	arg1	increase					1167:1174	a 57% increase	1161:1174	a 57% increase in growth inhibition	1161:1195	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	7	41	theme	thermo-chemical	921:935	arg1	treatment					937:945	This alkaline thermo-chemical treatment	907:945	This alkaline thermo-chemical treatment	907:945	This alkaline thermo-chemical treatment reconstituted both the nanoparticles and their bactericidal properties.
36433110	8	42	theme	growth	1179:1184	arg1	inhibition					1186:1195	growth inhibition	1179:1195	growth inhibition	1179:1195	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	8	43	theme	assay	1059:1063	arg1	results					1065:1071	The Staphylococcus aureus antibacterial assay results	1019:1071	The Staphylococcus aureus antibacterial assay results	1019:1071	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	6	44	theme	thermo-chemical	822:836	arg1	step					847:850	a second successful alkaline thermo-chemical regrowth step	793:850	a second successful alkaline thermo-chemical regrowth step	793:850	Thus, a second successful alkaline thermo-chemical regrowth step was added to the process to treat the obtained fibers.
36433110	3	45	theme	guar	469:472	arg1	membranes					503:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes	450:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles	450:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	8	46	from	increase	1167:1174	arg1	inhibition					1186:1195	growth inhibition	1179:1195	growth inhibition	1179:1195	The Staphylococcus aureus antibacterial assay results show that the membranes obtained after the alkaline thermo-chemical treatment presented a 57% increase in growth inhibition.
36433110	3	47	theme	gum/polyvinyl	474:486	arg1	membranes					503:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes	450:511	bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles	450:563	In this work, bio-nano-composite guar gum/polyvinyl alcohol (PVOH) membranes loaded with aluminum-doped zinc oxide nanoparticles were produced via electrospinning.
36433110	1	48	theme	antibiotic-resistant	122:141	arg1	infections					153:162	antibiotic-resistant bacterial infections	122:162	antibiotic-resistant bacterial infections	122:162	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
36433110	7	49	theme	alkaline	912:919	arg1	treatment					937:945	This alkaline thermo-chemical treatment	907:945	This alkaline thermo-chemical treatment	907:945	This alkaline thermo-chemical treatment reconstituted both the nanoparticles and their bactericidal properties.
36433110	0	50	theme	Composite	45:53	arg1	Fiber					55:59	Guar Gum/PVOH Composite Fiber	31:59	Guar Gum/PVOH Composite Fiber	31:59	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	0	51	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Synthesis of AZO-Np in Guar Gum/PVOH Composite Fiber Mats for Potential Bactericidal Release.
36433110	1	52	theme	bacterial	143:151	arg1	infections					153:162	antibiotic-resistant bacterial infections	122:162	antibiotic-resistant bacterial infections	122:162	Since the number of antibiotic-resistant bacterial infections is growing and cases are getting worse every year, the search for new alternative bactericidal wound dressing treatments is becoming crucial.
34647261	8	0	theme	fixed	943:947	arg1	material					934:941	Plant material	928:941	Plant material fixed with the RNAlater® solution	928:975	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	0	1	from	Mechanisms	18:27	arg1	Plants					54:59	Plants	54:59	Plants	54:59	Understanding the Mechanisms of Gravity Resistance in Plants.
34647261	4	2	from	resistance	525:534	arg1	plants					539:544	plants	539:544	plants	539:544	Here, we describe basic procedures for space-based experiments to study gravity resistance in plants.
34647261	6	3	from	fixatives	741:749	arg1	containers					781:790	appropriate sample storage containers	754:790	appropriate sample storage containers such as the Chemical Fixation Bag	754:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	6	3	from	fixatives	741:749	arg1	Bag					822:824	the Chemical Fixation Bag	800:824	the Chemical Fixation Bag	800:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	8	4	theme	RNA	1059:1061	arg1	properties					1030:1039	the mechanical properties	1015:1039	the mechanical properties of the cell wall	1015:1056	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	4	theme	RNA	1059:1061	arg1	extraction					1063:1072	RNA extraction	1059:1072	RNA extraction (which is necessary for gene-expression analysis)	1059:1122	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	5	theme	mechanical	1019:1028	arg1	properties					1030:1039	the mechanical properties	1015:1039	the mechanical properties of the cell wall	1015:1056	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	5	theme	mechanical	1019:1028	arg1	extraction					1063:1072	RNA extraction	1059:1072	RNA extraction (which is necessary for gene-expression analysis)	1059:1122	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	6	theme	enzyme	1138:1143	arg1	activity					1145:1152	the enzyme activity	1134:1152	the enzyme activity of cell wall proteins	1134:1174	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	4	7	theme	gravity	517:523	arg1	resistance					525:534	gravity resistance	517:534	gravity resistance in plants	517:544	Here, we describe basic procedures for space-based experiments to study gravity resistance in plants.
34647261	9	8	used	used	1278:1281	arg2	material					1257:1264	The plant material	1247:1264	The plant material	1247:1264	The plant material can also be used directly for microscopic observation of cellular components such as cortical microtubules.
34647261	5	9	theme	growing	616:622	arg1	period					624:629	the growing period	612:629	the growing period of the plants	612:643	An appropriate cultivation chamber must be selected according to the growing period of the plants and the purpose of the experiment.
34647261	7	10	theme	methods	875:881	arg1	methods					875:881	methods	875:881	methods	875:881	The material is then analyzed with a variety of methods, depending on the purpose of the experiment.
34647261	7	10	theme	methods	875:881	arg1	variety					864:870	a variety	862:870	a variety of methods	862:881	The material is then analyzed with a variety of methods, depending on the purpose of the experiment.
34647261	3	11	theme	large	362:366	arg1	number					368:373	a large number	360:373	a large number of constraints	360:388	Experiments carried out in space involve a large number of constraints and are quite different from ground-based experiments.
34647261	0	12	from	Plants	54:59	arg1	Mechanisms					18:27	the Mechanisms	14:27	the Mechanisms of Gravity Resistance in Plants	14:59	Understanding the Mechanisms of Gravity Resistance in Plants.
34647261	5	13	theme	experiment	668:677	arg1	period					624:629	the growing period	612:629	the growing period of the plants	612:643	An appropriate cultivation chamber must be selected according to the growing period of the plants and the purpose of the experiment.
34647261	5	13	theme	experiment	668:677	arg1	purpose					653:659	the purpose	649:659	the purpose of the experiment	649:677	An appropriate cultivation chamber must be selected according to the growing period of the plants and the purpose of the experiment.
34647261	6	14	theme	Fixation	813:820	arg1	Bag					822:824	the Chemical Fixation Bag	800:824	the Chemical Fixation Bag	800:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	8	15	theme	polysaccharides	1230:1244	arg1	compositions					1204:1215	compositions	1204:1215	compositions	1204:1215	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	15	theme	polysaccharides	1230:1244	arg1	levels					1193:1198	levels	1193:1198	levels	1193:1198	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	16	theme	cell	1048:1051	arg1	wall					1053:1056	the cell wall	1044:1056	the cell wall	1044:1056	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	6	17	theme	Chemical	804:811	arg1	Bag					822:824	the Chemical Fixation Bag	800:824	the Chemical Fixation Bag	800:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	8	18	theme	cell	1220:1223	arg1	polysaccharides					1230:1244	cell wall polysaccharides	1220:1244	cell wall polysaccharides	1220:1244	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	0	19	from	Resistance	40:49	arg1	Plants					54:59	Plants	54:59	Plants	54:59	Understanding the Mechanisms of Gravity Resistance in Plants.
34647261	5	20	theme	plants	638:643	arg1	period					624:629	the growing period	612:629	the growing period of the plants	612:643	An appropriate cultivation chamber must be selected according to the growing period of the plants and the purpose of the experiment.
34647261	5	20	theme	plants	638:643	arg1	purpose					653:659	the purpose	649:659	the purpose of the experiment	649:677	An appropriate cultivation chamber must be selected according to the growing period of the plants and the purpose of the experiment.
34647261	1	21	theme	gravity	76:82	arg1	resistance					84:93	gravity resistance	76:93	gravity resistance in plants	76:103	To understand gravity resistance in plants, it is necessary to analyze the changes induced when the magnitude of gravity in a growth environment is modified.
34647261	6	22	theme	plant	703:707	arg1	fixed					721:725	fixed	721:725	fixed	721:725	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	6	22	theme	plant	703:707	arg1	material					709:716	the plant material	699:716	the plant material	699:716	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	3	23	theme	constraints	378:388	arg1	number					368:373	a large number	360:373	a large number of constraints	360:388	Experiments carried out in space involve a large number of constraints and are quite different from ground-based experiments.
34647261	4	24	theme	space-based	484:494	arg1	experiments					496:506	space-based experiments	484:506	space-based experiments	484:506	Here, we describe basic procedures for space-based experiments to study gravity resistance in plants.
34647261	3	25	theme	ground-based	419:430	arg1	experiments					432:442	ground-based experiments	419:442	ground-based experiments	419:442	Experiments carried out in space involve a large number of constraints and are quite different from ground-based experiments.
34647261	8	26	theme	cell	1157:1160	arg1	proteins					1167:1174	cell wall proteins	1157:1174	cell wall proteins	1157:1174	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	9	27	theme	microscopic	1296:1306	arg1	observation					1308:1318	microscopic observation	1296:1318	microscopic observation of cellular components such as cortical microtubules	1296:1371	The plant material can also be used directly for microscopic observation of cellular components such as cortical microtubules.
34647261	8	28	theme	wall	1053:1056	arg1	properties					1030:1039	the mechanical properties	1015:1039	the mechanical properties of the cell wall	1015:1056	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	28	theme	wall	1053:1056	arg1	extraction					1063:1072	RNA extraction	1059:1072	RNA extraction (which is necessary for gene-expression analysis)	1059:1122	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	29	dep	levels	1193:1198	arg1	the					1189:1191	the	1189:1191	the	1189:1191	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	7	30	theme	experiment	916:925	arg1	purpose					901:907	the purpose	897:907	the purpose of the experiment	897:925	The material is then analyzed with a variety of methods, depending on the purpose of the experiment.
34647261	2	31	theme	resistance	296:305	arg1	mechanisms					307:316	gravity resistance mechanisms	288:316	gravity resistance mechanisms	288:316	Microgravity in space provides appropriate conditions for analyzing gravity resistance mechanisms.
34647261	2	32	from	Microgravity	220:231	arg1	space					236:240	space	236:240	space	236:240	Microgravity in space provides appropriate conditions for analyzing gravity resistance mechanisms.
34647261	1	33	theme	gravity	175:181	arg1	magnitude					162:170	the magnitude	158:170	the magnitude of gravity in a growth environment	158:205	To understand gravity resistance in plants, it is necessary to analyze the changes induced when the magnitude of gravity in a growth environment is modified.
34647261	6	34	theme	suitable	732:739	arg1	fixatives					741:749	suitable fixatives	732:749	suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag	732:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	8	35	theme	Plant	928:932	arg1	material					934:941	Plant material	928:941	Plant material fixed with the RNAlater® solution	928:975	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	2	36	theme	gravity	288:294	arg1	mechanisms					307:316	gravity resistance mechanisms	288:316	gravity resistance mechanisms	288:316	Microgravity in space provides appropriate conditions for analyzing gravity resistance mechanisms.
34647261	8	37	used	used	984:987	arg2	material					934:941	Plant material	928:941	Plant material fixed with the RNAlater® solution	928:975	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	38	theme	wall	1225:1228	arg1	polysaccharides					1230:1244	cell wall polysaccharides	1220:1244	cell wall polysaccharides	1220:1244	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	0	39	theme	Resistance	40:49	arg1	Mechanisms					18:27	the Mechanisms	14:27	the Mechanisms of Gravity Resistance in Plants	14:59	Understanding the Mechanisms of Gravity Resistance in Plants.
34647261	9	40	theme	cellular	1323:1330	arg1	microtubules					1360:1371	cortical microtubules	1351:1371	cortical microtubules	1351:1371	The plant material can also be used directly for microscopic observation of cellular components such as cortical microtubules.
34647261	9	40	theme	cellular	1323:1330	arg1	components					1332:1341	cellular components	1323:1341	cellular components such as cortical microtubules	1323:1371	The plant material can also be used directly for microscopic observation of cellular components such as cortical microtubules.
34647261	0	41	theme	Gravity	32:38	arg1	Resistance					40:49	Gravity Resistance	32:49	Gravity Resistance in Plants	32:59	Understanding the Mechanisms of Gravity Resistance in Plants.
34647261	9	42	theme	components	1332:1341	arg1	observation					1308:1318	microscopic observation	1296:1318	microscopic observation of cellular components such as cortical microtubules	1296:1371	The plant material can also be used directly for microscopic observation of cellular components such as cortical microtubules.
34647261	4	43	theme	basic	463:467	arg1	procedures					469:478	basic procedures	463:478	basic procedures for space-based experiments	463:506	Here, we describe basic procedures for space-based experiments to study gravity resistance in plants.
34647261	5	44	theme	appropriate	550:560	arg1	chamber					574:580	An appropriate cultivation chamber	547:580	An appropriate cultivation chamber	547:580	An appropriate cultivation chamber must be selected according to the growing period of the plants and the purpose of the experiment.
34647261	1	45	theme	growth	188:193	arg1	environment					195:205	a growth environment	186:205	a growth environment	186:205	To understand gravity resistance in plants, it is necessary to analyze the changes induced when the magnitude of gravity in a growth environment is modified.
34647261	8	46	with	fixed	943:947	arg1	solution					968:975	the RNAlater® solution	954:975	the RNAlater® solution	954:975	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	5	47	theme	cultivation	562:572	arg1	chamber					574:580	An appropriate cultivation chamber	547:580	An appropriate cultivation chamber	547:580	An appropriate cultivation chamber must be selected according to the growing period of the plants and the purpose of the experiment.
34647261	6	48	theme	appropriate	754:764	arg1	containers					781:790	appropriate sample storage containers	754:790	appropriate sample storage containers such as the Chemical Fixation Bag	754:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	6	48	theme	appropriate	754:764	arg1	Bag					822:824	the Chemical Fixation Bag	800:824	the Chemical Fixation Bag	800:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	9	49	theme	cortical	1351:1358	arg1	microtubules					1360:1371	cortical microtubules	1351:1371	cortical microtubules	1351:1371	The plant material can also be used directly for microscopic observation of cellular components such as cortical microtubules.
34647261	8	50	theme	gene-expression	1098:1112	arg1	analysis					1114:1121	gene-expression analysis	1098:1121	gene-expression analysis	1098:1121	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	51	theme	RNAlater®	958:966	arg1	solution					968:975	the RNAlater® solution	954:975	the RNAlater® solution	954:975	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	6	52	theme	sample	766:771	arg1	containers					781:790	appropriate sample storage containers	754:790	appropriate sample storage containers such as the Chemical Fixation Bag	754:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	6	52	theme	sample	766:771	arg1	Bag					822:824	the Chemical Fixation Bag	800:824	the Chemical Fixation Bag	800:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	2	53	theme	appropriate	251:261	arg1	conditions					263:272	appropriate conditions	251:272	appropriate conditions	251:272	Microgravity in space provides appropriate conditions for analyzing gravity resistance mechanisms.
34647261	6	54	theme	storage	773:779	arg1	containers					781:790	appropriate sample storage containers	754:790	appropriate sample storage containers such as the Chemical Fixation Bag	754:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	6	54	theme	storage	773:779	arg1	Bag					822:824	the Chemical Fixation Bag	800:824	the Chemical Fixation Bag	800:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
34647261	1	55	from	magnitude	162:170	arg1	environment					195:205	a growth environment	186:205	a growth environment	186:205	To understand gravity resistance in plants, it is necessary to analyze the changes induced when the magnitude of gravity in a growth environment is modified.
34647261	1	56	from	resistance	84:93	arg1	plants					98:103	plants	98:103	plants	98:103	To understand gravity resistance in plants, it is necessary to analyze the changes induced when the magnitude of gravity in a growth environment is modified.
34647261	3	57	from	experiments	432:442	arg1	different					404:412	different	404:412	different	404:412	Experiments carried out in space involve a large number of constraints and are quite different from ground-based experiments.
34647261	9	58	theme	plant	1251:1255	arg1	material					1257:1264	The plant material	1247:1264	The plant material	1247:1264	The plant material can also be used directly for microscopic observation of cellular components such as cortical microtubules.
34647261	8	59	theme	proteins	1167:1174	arg1	activity					1145:1152	the enzyme activity	1134:1152	the enzyme activity of cell wall proteins	1134:1174	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	8	60	theme	wall	1162:1165	arg1	proteins					1167:1174	cell wall proteins	1157:1174	cell wall proteins	1157:1174	Plant material fixed with the RNAlater® solution can be used sequentially to determine the mechanical properties of the cell wall, RNA extraction (which is necessary for gene-expression analysis), estimate the enzyme activity of cell wall proteins, and measure the levels and compositions of cell wall polysaccharides.
34647261	6	61	with	fixed	721:725	arg1	fixatives					741:749	suitable fixatives	732:749	suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag	732:824	After cultivation, the plant material is fixed with suitable fixatives in appropriate sample storage containers such as the Chemical Fixation Bag.
37111992	0	0	theme	Arabic	83:88	arg1	Composites					90:99	Alginate-Gum Arabic Composites	70:99	Alginate-Gum Arabic Composites	70:99	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	5	1	theme	cryogels	793:800	arg1	character					776:784	the bioactive character	762:784	the bioactive character of the cryogels through the development of the apatite layer	762:845	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	2	from	immersion	883:891	arg1	fluid					911:915	simulated body fluid	896:915	simulated body fluid	896:915	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	2	3	theme	anionic	386:392	arg1	polysaccharides					394:408	the anionic polysaccharides	382:408	the anionic polysaccharides	382:408	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	4	4	theme	SEM	606:608	arg1	investigations					610:623	SEM investigations	606:623	SEM investigations	606:623	In addition, SEM investigations revealed a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering.
37111992	0	5	theme	Alginate-Gum	70:81	arg1	Composites					90:99	Alginate-Gum Arabic Composites	70:99	Alginate-Gum Arabic Composites	70:99	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	6	6	theme	cryogel	1128:1134	arg1	composites					1136:1145	alginate-gum arabic cryogel composites	1108:1145	alginate-gum arabic cryogel composites	1108:1145	Cytotoxicity tests performed on fibroblast cells demonstrated the non-toxic effect of alginate-gum arabic cryogel composites.
37111992	6	7	theme	alginate-gum	1108:1119	arg1	composites					1136:1145	alginate-gum arabic cryogel composites	1108:1145	alginate-gum arabic cryogel composites	1108:1145	Cytotoxicity tests performed on fibroblast cells demonstrated the non-toxic effect of alginate-gum arabic cryogel composites.
37111992	7	8	theme	high	1217:1220	arg1	content					1233:1239	a high gum arabic content	1215:1239	a high gum arabic content	1215:1239	In addition, an increase in flexibility was noted for samples with a high gum arabic content, which determines an appropriate environment to promote tissue regeneration.
37111992	6	9	theme	Cytotoxicity	1022:1033	arg1	tests					1035:1039	Cytotoxicity tests	1022:1039	Cytotoxicity tests performed on fibroblast cells	1022:1069	Cytotoxicity tests performed on fibroblast cells demonstrated the non-toxic effect of alginate-gum arabic cryogel composites.
37111992	2	10	theme	external	315:322	arg1	protocol					344:351	an external ionic cross-linking protocol	312:351	an external ionic cross-linking protocol	312:351	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	5	11	theme	calcium	965:971	arg1	phosphate					973:981	calcium phosphate	965:981	calcium phosphate	965:981	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	0	12	theme	Composites	90:99	arg1	Cryogels					23:30	Anionic Polysaccharide Cryogels	0:30	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.	0:100	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	12	theme	Composites	90:99	arg1	Behavior					58:65	In Vitro Behavior	49:65	In Vitro Behavior of Alginate-Gum Arabic Composites	49:99	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	12	theme	Composites	90:99	arg1	Interaction					33:43	Interaction	33:43	Interaction	33:43	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	6	13	theme	arabic	1121:1126	arg1	composites					1136:1145	alginate-gum arabic cryogel composites	1108:1145	alginate-gum arabic cryogel composites	1108:1145	Cytotoxicity tests performed on fibroblast cells demonstrated the non-toxic effect of alginate-gum arabic cryogel composites.
37111992	4	14	from	scaffold	701:708	arg1	engineering					720:730	tissue engineering	713:730	tissue engineering	713:730	In addition, SEM investigations revealed a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering.
37111992	5	15	theme	bioactive	766:774	arg1	character					776:784	the bioactive character	762:784	the bioactive character of the cryogels through the development of the apatite layer	762:845	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	7	16	theme	tissue	1297:1302	arg1	regeneration					1304:1315	tissue regeneration	1297:1315	tissue regeneration	1297:1315	In addition, an increase in flexibility was noted for samples with a high gum arabic content, which determines an appropriate environment to promote tissue regeneration.
37111992	5	17	theme	apatite	833:839	arg1	layer					841:845	the apatite layer	829:845	the apatite layer	829:845	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	3	18	theme	structural	432:441	arg1	features					443:450	The structural features	428:450	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis	428:505	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	8	19	theme	systems	1495:1501	arg1	regeneration					1420:1431	the regeneration	1416:1431	the regeneration of soft tissues, wound management, or controlled drug release systems	1416:1501	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	4	20	theme	suitable	687:694	arg1	structure					677:685	a porous, interconnected, and well-defined structure	634:685	a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering	634:730	In addition, SEM investigations revealed a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering.
37111992	0	21	theme	Polysaccharide	8:21	arg1	Cryogels					23:30	Anionic Polysaccharide Cryogels	0:30	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.	0:100	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	21	theme	Polysaccharide	8:21	arg1	Behavior					58:65	In Vitro Behavior	49:65	In Vitro Behavior of Alginate-Gum Arabic Composites	49:99	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	21	theme	Polysaccharide	8:21	arg1	Interaction					33:43	Interaction	33:43	Interaction	33:43	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	5	22	dep	in	737:738	arg1	vitro					740:744	vitro	740:744	vitro	740:744	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	23	theme	calcium	1005:1011	arg1	calcium					1005:1011	calcium	1005:1011	calcium	1005:1011	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	23	theme	calcium	1005:1011	arg1	amount					995:1000	a small amount	987:1000	a small amount of calcium	987:1011	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	23	theme	calcium	1005:1011	arg1	phosphate					973:981	calcium phosphate	965:981	calcium phosphate	965:981	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	24	theme	stable	949:954	arg1	phase					956:960	a stable phase	947:960	a stable phase of calcium phosphate and a small amount of calcium oxalate	947:1019	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	6	25	theme	non-toxic	1088:1096	arg1	effect					1098:1103	the non-toxic effect	1084:1103	the non-toxic effect of alginate-gum arabic cryogel composites	1084:1145	Cytotoxicity tests performed on fibroblast cells demonstrated the non-toxic effect of alginate-gum arabic cryogel composites.
37111992	0	26	theme	Anionic	0:6	arg1	Cryogels					23:30	Anionic Polysaccharide Cryogels	0:30	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.	0:100	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	26	theme	Anionic	0:6	arg1	Behavior					58:65	In Vitro Behavior	49:65	In Vitro Behavior of Alginate-Gum Arabic Composites	49:99	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	26	theme	Anionic	0:6	arg1	Interaction					33:43	Interaction	33:43	Interaction	33:43	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	2	27	with	composites	248:257	arg1	ratios					285:290	different gum arabic ratios	264:290	different gum arabic ratios	264:290	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	5	28	theme	phase	956:960	arg1	formation					934:942	the formation	930:942	the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate	930:1019	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	1	29	theme	synthetic	193:201	arg1	matrix					217:222	a synthetic extracellular matrix	191:222	a synthetic extracellular matrix	191:222	In the present study, polysaccharide-based cryogels demonstrate their potential to mimic a synthetic extracellular matrix.
37111992	5	30	theme	layer	841:845	arg1	development					814:824	the development	810:824	the development of the apatite layer	810:845	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	7	31	theme	appropriate	1262:1272	arg1	environment					1274:1284	an appropriate environment	1259:1284	an appropriate environment to promote tissue regeneration	1259:1315	In addition, an increase in flexibility was noted for samples with a high gum arabic content, which determines an appropriate environment to promote tissue regeneration.
37111992	2	32	theme	cross-linking	330:342	arg1	protocol					344:351	an external ionic cross-linking protocol	312:351	an external ionic cross-linking protocol	312:351	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	1	33	theme	extracellular	203:215	arg1	matrix					217:222	a synthetic extracellular matrix	191:222	a synthetic extracellular matrix	191:222	In the present study, polysaccharide-based cryogels demonstrate their potential to mimic a synthetic extracellular matrix.
37111992	5	34	theme	in	737:738	arg1	tests					746:750	The in vitro tests	733:750	The in vitro tests	733:750	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	8	35	theme	controlled	1471:1480	arg1	systems					1495:1501	controlled drug release systems	1471:1501	controlled drug release systems	1471:1501	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	2	36	theme	ionic	324:328	arg1	protocol					344:351	an external ionic cross-linking protocol	312:351	an external ionic cross-linking protocol	312:351	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	5	37	theme	body	906:909	arg1	fluid					911:915	simulated body fluid	896:915	simulated body fluid	896:915	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	38	theme	phosphate	973:981	arg1	phase					956:960	a stable phase	947:960	a stable phase of calcium phosphate and a small amount of calcium oxalate	947:1019	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	6	39	theme	fibroblast	1054:1063	arg1	cells					1065:1069	fibroblast cells	1054:1069	fibroblast cells	1054:1069	Cytotoxicity tests performed on fibroblast cells demonstrated the non-toxic effect of alginate-gum arabic cryogel composites.
37111992	2	40	theme	different	264:272	arg1	ratios					285:290	different gum arabic ratios	264:290	different gum arabic ratios	264:290	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	3	41	theme	Raman	471:475	arg1	analysis					498:505	FT-IR, Raman, and MAS NMR spectra analysis	464:505	FT-IR, Raman, and MAS NMR spectra analysis	464:505	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	3	42	theme	MAS	482:484	arg1	spectra					490:496	FT-IR, Raman, and MAS NMR spectra	464:496	spectra	490:496	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	3	43	theme	FT-IR	464:468	arg1	Raman					471:475	FT-IR, Raman, and MAS NMR spectra	464:496	Raman	471:475	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	8	44	theme	release	1487:1493	arg1	systems					1495:1501	controlled drug release systems	1471:1501	controlled drug release systems	1471:1501	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	0	45	dep	In	49:50	arg1	Vitro					52:56	Vitro	52:56	Vitro	52:56	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	5	46	theme	samples	869:875	arg1	surface					854:860	the surface	850:860	the surface of the samples	850:875	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	3	47	theme	NMR	486:488	arg1	spectra					490:496	FT-IR, Raman, and MAS NMR spectra	464:496	spectra	490:496	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	8	48	theme	drug	1482:1485	arg1	systems					1495:1501	controlled drug release systems	1471:1501	controlled drug release systems	1471:1501	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	8	49	theme	tissues	1441:1447	arg1	regeneration					1420:1431	the regeneration	1416:1431	the regeneration of soft tissues, wound management, or controlled drug release systems	1416:1501	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	6	50	theme	composites	1136:1145	arg1	effect					1098:1103	the non-toxic effect	1084:1103	the non-toxic effect of alginate-gum arabic cryogel composites	1084:1145	Cytotoxicity tests performed on fibroblast cells demonstrated the non-toxic effect of alginate-gum arabic cryogel composites.
37111992	1	51	theme	present	109:115	arg1	study					117:121	the present study	105:121	the present study	105:121	In the present study, polysaccharide-based cryogels demonstrate their potential to mimic a synthetic extracellular matrix.
37111992	0	52	theme	In	49:50	arg1	Cryogels					23:30	Anionic Polysaccharide Cryogels	0:30	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.	0:100	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	52	theme	In	49:50	arg1	Behavior					58:65	In Vitro Behavior	49:65	In Vitro Behavior of Alginate-Gum Arabic Composites	49:99	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	2	53	theme	Alginate-based	225:238	arg1	composites					248:257	Alginate-based cryogel composites	225:257	Alginate-based cryogel composites with different gum arabic ratios	225:290	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	2	54	theme	cryogel	240:246	arg1	composites					248:257	Alginate-based cryogel composites	225:257	Alginate-based cryogel composites with different gum arabic ratios	225:290	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	3	55	theme	chelation	524:532	arg1	process					556:562	the main process	547:562	the main process linking the two biopolymers	547:590	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	3	55	theme	chelation	524:532	arg1	mechanism					534:542	a chelation mechanism	522:542	a chelation mechanism	522:542	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	8	56	theme	management	1456:1465	arg1	regeneration					1420:1431	the regeneration	1416:1431	the regeneration of soft tissues, wound management, or controlled drug release systems	1416:1501	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	8	57	theme	wound	1450:1454	arg1	management					1456:1465	wound management	1450:1465	wound management	1450:1465	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	5	58	theme	amount	995:1000	arg1	phase					956:960	a stable phase	947:960	a stable phase of calcium phosphate and a small amount of calcium oxalate	947:1019	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	7	59	from	increase	1164:1171	arg1	flexibility					1176:1186	flexibility	1176:1186	flexibility	1176:1186	In addition, an increase in flexibility was noted for samples with a high gum arabic content, which determines an appropriate environment to promote tissue regeneration.
37111992	5	60	dep	phosphate	973:981	arg1	oxalate					1013:1019	oxalate	1013:1019	oxalate	1013:1019	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	61	theme	simulated	896:904	arg1	fluid					911:915	simulated body fluid	896:915	simulated body fluid	896:915	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	4	62	theme	porous	636:641	arg1	structure					677:685	a porous, interconnected, and well-defined structure	634:685	a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering	634:730	In addition, SEM investigations revealed a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering.
37111992	5	63	theme	small	989:993	arg1	calcium					1005:1011	calcium	1005:1011	calcium	1005:1011	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	5	63	theme	small	989:993	arg1	amount					995:1000	a small amount	987:1000	a small amount of calcium	987:1011	The in vitro tests confirmed the bioactive character of the cryogels through the development of the apatite layer on the surface of the samples after immersion in simulated body fluid, identifying the formation of a stable phase of calcium phosphate and a small amount of calcium oxalate.
37111992	0	64	dep	Cryogels	23:30	arg1	Cryogels					23:30	Anionic Polysaccharide Cryogels	0:30	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.	0:100	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	64	dep	Cryogels	23:30	arg1	Behavior					58:65	In Vitro Behavior	49:65	In Vitro Behavior of Alginate-Gum Arabic Composites	49:99	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	0	64	dep	Cryogels	23:30	arg1	Interaction					33:43	Interaction	33:43	Interaction	33:43	Anionic Polysaccharide Cryogels: Interaction and In Vitro Behavior of Alginate-Gum Arabic Composites.
37111992	7	65	theme	arabic	1226:1231	arg1	content					1233:1239	a high gum arabic content	1215:1239	a high gum arabic content	1215:1239	In addition, an increase in flexibility was noted for samples with a high gum arabic content, which determines an appropriate environment to promote tissue regeneration.
37111992	1	66	theme	polysaccharide-based	124:143	arg1	cryogels					145:152	polysaccharide-based cryogels	124:152	polysaccharide-based cryogels	124:152	In the present study, polysaccharide-based cryogels demonstrate their potential to mimic a synthetic extracellular matrix.
37111992	8	67	theme	obtained	1328:1335	arg1	biomaterials					1337:1348	The newly obtained biomaterials	1318:1348	The newly obtained biomaterials that exhibit all these properties	1318:1382	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	4	68	theme	well-defined	664:675	arg1	structure					677:685	a porous, interconnected, and well-defined structure	634:685	a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering	634:730	In addition, SEM investigations revealed a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering.
37111992	2	69	theme	arabic	278:283	arg1	ratios					285:290	different gum arabic ratios	264:290	different gum arabic ratios	264:290	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	3	70	theme	spectra	490:496	arg1	analysis					498:505	FT-IR, Raman, and MAS NMR spectra analysis	464:505	FT-IR, Raman, and MAS NMR spectra analysis	464:505	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	4	71	theme	tissue	713:718	arg1	engineering					720:730	tissue engineering	713:730	tissue engineering	713:730	In addition, SEM investigations revealed a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering.
37111992	4	72	theme	interconnected	644:657	arg1	structure					677:685	a porous, interconnected, and well-defined structure	634:685	a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering	634:730	In addition, SEM investigations revealed a porous, interconnected, and well-defined structure suitable as a scaffold in tissue engineering.
37111992	7	73	with	samples	1202:1208	arg1	content					1233:1239	a high gum arabic content	1215:1239	a high gum arabic content	1215:1239	In addition, an increase in flexibility was noted for samples with a high gum arabic content, which determines an appropriate environment to promote tissue regeneration.
37111992	2	74	theme	gum	274:276	arg1	ratios					285:290	different gum arabic ratios	264:290	different gum arabic ratios	264:290	Alginate-based cryogel composites with different gum arabic ratios were synthesized by an external ionic cross-linking protocol, and the interaction between the anionic polysaccharides was investigated.
37111992	3	75	theme	main	551:554	arg1	process					556:562	the main process	547:562	the main process linking the two biopolymers	547:590	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	3	75	theme	main	551:554	arg1	mechanism					534:542	a chelation mechanism	522:542	a chelation mechanism	522:542	The structural features provided by FT-IR, Raman, and MAS NMR spectra analysis indicated that a chelation mechanism is the main process linking the two biopolymers.
37111992	8	76	theme	soft	1436:1439	arg1	tissues					1441:1447	soft tissues	1436:1447	soft tissues	1436:1447	The newly obtained biomaterials that exhibit all these properties can be successfully involved in the regeneration of soft tissues, wound management, or controlled drug release systems.
37111992	7	77	theme	gum	1222:1224	arg1	content					1233:1239	a high gum arabic content	1215:1239	a high gum arabic content	1215:1239	In addition, an increase in flexibility was noted for samples with a high gum arabic content, which determines an appropriate environment to promote tissue regeneration.
36559750	7	0	from	particles	1318:1326	arg1	formation					1249:1257	the formation	1245:1257	the formation of strong composite coatings from polymer(s) and soil/sand particles	1245:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	7	0	from	particles	1318:1326	arg1	coatings					1279:1286	strong composite coatings	1262:1286	strong composite coatings from polymer(s) and soil/sand particles	1262:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	6	1	theme	0.2	1115:1117	arg1	M					1119:1119	M	1119:1119	M	1119:1119	(c) A complete dissociation of polycomplexes to the initial components is achieved in a 0.2 M NaCl solution.
36559750	4	2	with	polycomplexes	774:786	arg1	excess					796:801	an excess	793:801	an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively)	793:885	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	1	3	theme	interpolyelectrolyte	275:294	arg1	complex					296:302	an interpolyelectrolyte complex	272:302	an interpolyelectrolyte complex	272:302	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	7	4	dep	lead	1237:1240	arg1	d					1137:1137	d	1137:1137	d	1137:1137	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	7	5	from	formation	1249:1257	arg1	s					1301:1301	polymer(s)	1293:1302	polymer(s)	1293:1302	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	7	5	from	formation	1249:1257	arg1	particles					1318:1326	soil/sand particles	1308:1326	soil/sand particles	1308:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	7	6	with	treatment	1150:1158	arg1	solutions					1179:1187	1 wt% aqueous solutions	1165:1187	1 wt% aqueous solutions	1165:1187	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	2	7	theme	Aqueous	305:311	arg1	solutions					313:321	Aqueous solutions	305:321	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers	305:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	1	8	theme	quaternized	192:202	arg1	ethoxylate					227:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	1	8	theme	quaternized	192:202	arg1	polysaccharide					176:189	a cationic polysaccharide	165:189	a cationic polysaccharide	165:189	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	4	9	theme	transparent	892:902	arg1	solutions					904:912	transparent solutions	892:912	transparent solutions	892:912	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	10	10	theme	individual	1562:1571	arg1	polysaccharides					1573:1587	The individual polysaccharides	1558:1587	(g) The individual polysaccharides	1554:1587	(g) The individual polysaccharides demonstrate a negligible toxicity to Gram-negative and Gram-positive bacteria and yeast.
36559750	10	10	theme	individual	1562:1571	arg1	g					1555:1555	g	1555:1555	g	1555:1555	(g) The individual polysaccharides demonstrate a negligible toxicity to Gram-negative and Gram-positive bacteria and yeast.
36559750	1	11	theme	cellulose	217:225	arg1	ethoxylate					227:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	1	11	theme	cellulose	217:225	arg1	polysaccharide					176:189	a cationic polysaccharide	165:189	a cationic polysaccharide	165:189	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	11	12	theme	polycomplexes	1777:1789	arg1	degradation					1738:1748	the degradation	1734:1748	the degradation of the polysaccharides and polycomplexes	1734:1789	(h) The addition of Bacillus subtilis culture initiates the degradation of the polysaccharides and polycomplexes.
36559750	2	13	theme	initial	330:336	arg1	polysaccharides					338:352	polysaccharides	338:352	polysaccharides	338:352	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	3	14	with	degradation	702:712	arg1	conclusions					738:748	the following main conclusions	719:748	the following main conclusions	719:748	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	10	15	theme	negligible	1603:1612	arg1	toxicity					1614:1621	a negligible toxicity	1601:1621	a negligible toxicity to Gram-negative and Gram-positive bacteria and yeast	1601:1675	(g) The individual polysaccharides demonstrate a negligible toxicity to Gram-negative and Gram-positive bacteria and yeast.
36559750	1	16	theme	aqueous	242:248	arg1	solution					250:257	aqueous solution	242:257	aqueous solution	242:257	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	2	17	dep	polysaccharides	338:352	arg1	the					326:328	the	326:328	the	326:328	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	3	18	theme	mechanical	636:645	arg1	assessment					656:665	mechanical strength assessment	636:665	mechanical strength assessment	636:665	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	3	19	theme	Physicochemical	495:509	arg1	properties					538:547	Physicochemical, mechanical and biological properties	495:547	Physicochemical, mechanical and biological properties of the polymers and coatings	495:576	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	11	20	theme	culture	1716:1722	arg1	h					1679:1679	h	1679:1679	h	1679:1679	(h) The addition of Bacillus subtilis culture initiates the degradation of the polysaccharides and polycomplexes.
36559750	11	20	theme	culture	1716:1722	arg1	addition					1686:1693	The addition	1682:1693	(h) The addition of Bacillus subtilis culture	1678:1722	(h) The addition of Bacillus subtilis culture initiates the degradation of the polysaccharides and polycomplexes.
36559750	7	21	theme	drying	1230:1235	arg1	treatment					1150:1158	Soil/sand treatment	1140:1158	Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying	1140:1235	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	3	22	with	viscosimetry	622:633	arg1	conclusions					738:748	the following main conclusions	719:748	the following main conclusions	719:748	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	11	23	theme	polysaccharides	1757:1771	arg1	degradation					1738:1748	the degradation	1734:1748	the degradation of the polysaccharides and polycomplexes	1734:1789	(h) The addition of Bacillus subtilis culture initiates the degradation of the polysaccharides and polycomplexes.
36559750	4	24	theme	units	826:830	arg1	excess					796:801	an excess	793:801	an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively)	793:885	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	4	25	theme	cationic	806:813	arg1	units					826:830	cationic or anionic units	806:830	cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively)	806:885	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	9	26	theme	lower	1527:1531	arg1	resistance					1533:1542	a much lower resistance	1520:1542	a much lower resistance to water	1520:1551	(f) The polymer-soil coatings are stable towards re-watering, while the polymer-sand coatings show a much lower resistance to water.
36559750	10	27	theme	Gram-positive	1644:1656	arg1	bacteria					1658:1665	Gram-negative and Gram-positive bacteria	1626:1665	Gram-negative and Gram-positive bacteria	1626:1665	(g) The individual polysaccharides demonstrate a negligible toxicity to Gram-negative and Gram-positive bacteria and yeast.
36559750	3	28	with	assessment	656:665	arg1	conclusions					738:748	the following main conclusions	719:748	the following main conclusions	719:748	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	1	29	theme	linear	84:89	arg1	polysaccharide					99:112	A linear anionic polysaccharide	82:112	A linear anionic polysaccharide	82:112	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	1	29	theme	linear	84:89	arg1	alginate					122:129	sodium alginate	115:129	sodium alginate	115:129	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	2	30	theme	water	480:484	arg1	erosion					486:492	water erosion	480:492	water erosion	480:492	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	10	31	theme	Gram-negative	1626:1638	arg1	bacteria					1658:1665	Gram-negative and Gram-positive bacteria	1626:1665	Gram-negative and Gram-positive bacteria	1626:1665	(g) The individual polysaccharides demonstrate a negligible toxicity to Gram-negative and Gram-positive bacteria and yeast.
36559750	4	32	dep	form	887:890	arg1	a					752:752	a	752:752	a	752:752	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	2	33	theme	sand	467:470	arg1	stabilization					441:453	the stabilization	437:453	the stabilization of soil and sand against water erosion	437:492	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	8	34	theme	stronger	1361:1368	arg1	coatings					1370:1377	stronger coatings	1361:1377	stronger coatings	1361:1377	(e) Cationic polycomplexes form stronger coatings in comparison with anionic polycomplexes.
36559750	2	35	theme	soil	458:461	arg1	stabilization					441:453	the stabilization	437:453	the stabilization of soil and sand against water erosion	437:492	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	7	36	theme	wt	1167:1168	arg1	solutions					1179:1187	1 wt% aqueous solutions	1165:1187	1 wt% aqueous solutions	1165:1187	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	1	37	theme	sodium	115:120	arg1	polysaccharide					99:112	A linear anionic polysaccharide	82:112	A linear anionic polysaccharide	82:112	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	1	37	theme	sodium	115:120	arg1	alginate					122:129	sodium alginate	115:129	sodium alginate	115:129	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	0	38	theme	Sand	76:79	arg1	Stabilization					50:62	Anti-Erosion Stabilization	37:62	Anti-Erosion Stabilization of Soil and Sand	37:79	Biodegradable Interpolycomplexes for Anti-Erosion Stabilization of Soil and Sand.
36559750	3	39	with	analysis	612:619	arg1	conclusions					738:748	the following main conclusions	719:748	the following main conclusions	719:748	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	6	40	theme	polycomplexes	1058:1070	arg1	dissociation					1042:1053	A complete dissociation	1031:1053	A complete dissociation of polycomplexes to the initial components	1031:1096	(c) A complete dissociation of polycomplexes to the initial components is achieved in a 0.2 M NaCl solution.
36559750	3	41	theme	cell-mediated	688:700	arg1	degradation					702:712	cell-mediated degradation	688:712	cell-mediated degradation	688:712	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	3	42	theme	biological	527:536	arg1	properties					538:547	Physicochemical, mechanical and biological properties	495:547	Physicochemical, mechanical and biological properties of the polymers and coatings	495:576	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	0	43	theme	Soil	67:70	arg1	Stabilization					50:62	Anti-Erosion Stabilization	37:62	Anti-Erosion Stabilization of Soil and Sand	37:79	Biodegradable Interpolycomplexes for Anti-Erosion Stabilization of Soil and Sand.
36559750	7	44	theme	coatings	1279:1286	arg1	formation					1249:1257	the formation	1245:1257	the formation of strong composite coatings from polymer(s) and soil/sand particles	1245:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	3	45	theme	cell	668:671	arg1	viability					673:681	cell viability	668:681	cell viability	668:681	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	13	46	theme	protective	2015:2024	arg1	coatings					2026:2033	biodegradable protective coatings	2001:2033	biodegradable protective coatings	2001:2033	The results of the work are of importance for constructing water-resistant, low toxicity and biodegradable protective coatings for soil and sand.
36559750	3	47	theme	mechanical	512:521	arg1	properties					538:547	Physicochemical, mechanical and biological properties	495:547	Physicochemical, mechanical and biological properties of the polymers and coatings	495:576	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	7	48	theme	strong	1262:1267	arg1	coatings					1279:1286	strong composite coatings	1262:1286	strong composite coatings from polymer(s) and soil/sand particles	1262:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	7	49	theme	polymer	1293:1299	arg1	s					1301:1301	polymer(s)	1293:1302	polymer(s)	1293:1302	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	3	50	theme	polymers	556:563	arg1	properties					538:547	Physicochemical, mechanical and biological properties	495:547	Physicochemical, mechanical and biological properties of the polymers and coatings	495:576	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	5	51	from	decrease	979:986	arg1	viscosity					995:1003	the viscosity	991:1003	the viscosity of polymer solutions	991:1024	(b) The complexation results in a decrease in the viscosity of polymer solutions.
36559750	6	52	theme	M	1119:1119	arg1	solution					1126:1133	a 0.2 M NaCl solution	1113:1133	a 0.2 M NaCl solution	1113:1133	(c) A complete dissociation of polycomplexes to the initial components is achieved in a 0.2 M NaCl solution.
36559750	7	53	theme	polycomplexes	1204:1216	arg1	treatment					1150:1158	Soil/sand treatment	1140:1158	Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying	1140:1235	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	13	54	theme	low	1984:1986	arg1	toxicity					1988:1995	low toxicity	1984:1995	low toxicity	1984:1995	The results of the work are of importance for constructing water-resistant, low toxicity and biodegradable protective coatings for soil and sand.
36559750	2	55	theme	cationic	394:401	arg1	polymers					414:421	the cationic or anionic polymers	390:421	the cationic or anionic polymers	390:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	11	56	dep	Bacillus	1698:1705	arg1	subtilis					1707:1714	subtilis	1707:1714	subtilis	1707:1714	(h) The addition of Bacillus subtilis culture initiates the degradation of the polysaccharides and polycomplexes.
36559750	4	57	theme	stable-in-time	917:930	arg1	dispersions					932:942	stable-in-time dispersions	917:942	stable-in-time dispersions	917:942	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	4	58	dep	units	826:830	arg1	"					856:856	"anionic"	848:856	"anionic"	848:856	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	4	58	dep	units	826:830	arg1	"					842:842	"cationic"	833:842	"cationic"	833:842	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	12	59	from	polysaccharides	1807:1821	arg1	Films					1796:1800	Films	1796:1800	(i) Films from polysaccharides and polycomplexes	1792:1839	(i) Films from polysaccharides and polycomplexes decompose down to small fragments after being in soil for 6 weeks.
36559750	3	60	theme	coatings	569:576	arg1	properties					538:547	Physicochemical, mechanical and biological properties	495:547	Physicochemical, mechanical and biological properties of the polymers and coatings	495:576	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	1	61	theme	hydroxyethyl	204:215	arg1	ethoxylate					227:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	1	61	theme	hydroxyethyl	204:215	arg1	polysaccharide					176:189	a cationic polysaccharide	165:189	a cationic polysaccharide	165:189	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	7	62	theme	Soil/sand	1140:1148	arg1	treatment					1150:1158	Soil/sand treatment	1140:1158	Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying	1140:1235	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	2	63	theme	polycomplexes	358:370	arg1	solutions					313:321	Aqueous solutions	305:321	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers	305:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	4	64	dep	"	842:842	arg1	polycomplexes					858:870	polycomplexes	858:870	polycomplexes	858:870	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	11	65	theme	Bacillus	1698:1705	arg1	culture					1716:1722	Bacillus subtilis culture	1698:1722	Bacillus subtilis culture	1698:1722	(h) The addition of Bacillus subtilis culture initiates the degradation of the polysaccharides and polycomplexes.
36559750	8	66	with	comparison	1382:1391	arg1	polycomplexes					1406:1418	anionic polycomplexes	1398:1418	anionic polycomplexes	1398:1418	(e) Cationic polycomplexes form stronger coatings in comparison with anionic polycomplexes.
36559750	13	67	theme	work	1927:1930	arg1	results					1912:1918	The results	1908:1918	The results of the work	1908:1930	The results of the work are of importance for constructing water-resistant, low toxicity and biodegradable protective coatings for soil and sand.
36559750	2	68	theme	polysaccharides	338:352	arg1	solutions					313:321	Aqueous solutions	305:321	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers	305:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	3	69	theme	strength	647:654	arg1	assessment					656:665	mechanical strength assessment	636:665	mechanical strength assessment	636:665	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	9	70	theme	polymer-soil	1429:1440	arg1	f					1422:1422	f	1422:1422	f	1422:1422	(f) The polymer-soil coatings are stable towards re-watering, while the polymer-sand coatings show a much lower resistance to water.
36559750	9	70	theme	polymer-soil	1429:1440	arg1	stable					1455:1460	stable	1455:1460	stable	1455:1460	(f) The polymer-soil coatings are stable towards re-watering, while the polymer-sand coatings show a much lower resistance to water.
36559750	9	70	theme	polymer-soil	1429:1440	arg1	coatings					1442:1449	The polymer-soil coatings	1425:1449	(f) The polymer-soil coatings	1421:1449	(f) The polymer-soil coatings are stable towards re-watering, while the polymer-sand coatings show a much lower resistance to water.
36559750	12	71	from	polycomplexes	1827:1839	arg1	Films					1796:1800	Films	1796:1800	(i) Films from polysaccharides and polycomplexes	1792:1839	(i) Films from polysaccharides and polycomplexes decompose down to small fragments after being in soil for 6 weeks.
36559750	4	72	theme	Non-stoichiometric	755:772	arg1	polycomplexes					774:786	Non-stoichiometric polycomplexes	755:786	Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively)	755:885	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	3	73	theme	gravimetric	600:610	arg1	analysis					612:619	gravimetric analysis	600:619	gravimetric analysis	600:619	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	5	74	dep	results	966:972	arg1	b					946:946	b	946:946	b	946:946	(b) The complexation results in a decrease in the viscosity of polymer solutions.
36559750	7	75	theme	further	1222:1228	arg1	drying					1230:1235	further drying	1222:1235	further drying	1222:1235	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	9	76	theme	polymer-sand	1493:1504	arg1	coatings					1506:1513	the polymer-sand coatings	1489:1513	the polymer-sand coatings	1489:1513	(f) The polymer-soil coatings are stable towards re-watering, while the polymer-sand coatings show a much lower resistance to water.
36559750	6	77	theme	complete	1033:1040	arg1	dissociation					1042:1053	A complete dissociation	1031:1053	A complete dissociation of polycomplexes to the initial components	1031:1096	(c) A complete dissociation of polycomplexes to the initial components is achieved in a 0.2 M NaCl solution.
36559750	2	78	used	used	428:431	arg2	solutions					313:321	Aqueous solutions	305:321	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers	305:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	3	79	with	viability	673:681	arg1	conclusions					738:748	the following main conclusions	719:748	the following main conclusions	719:748	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	1	80	theme	cationic	167:174	arg1	ethoxylate					227:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	quaternized hydroxyethyl cellulose ethoxylate	192:236	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	1	80	theme	cationic	167:174	arg1	polysaccharide					176:189	a cationic polysaccharide	165:189	a cationic polysaccharide	165:189	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	2	81	with	polysaccharides	338:352	arg1	excess					380:385	an excess	377:385	an excess of the cationic or anionic polymers	377:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	7	82	theme	polymers	1192:1199	arg1	treatment					1150:1158	Soil/sand treatment	1140:1158	Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying	1140:1235	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	4	83	theme	cationic	834:841	arg1	"					842:842	"cationic"	833:842	"cationic"	833:842	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	2	84	with	polycomplexes	358:370	arg1	excess					380:385	an excess	377:385	an excess of the cationic or anionic polymers	377:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	4	85	theme	anionic	818:824	arg1	units					826:830	cationic or anionic units	806:830	cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively)	806:885	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	5	86	theme	polymer	1008:1014	arg1	solutions					1016:1024	polymer solutions	1008:1024	polymer solutions	1008:1024	(b) The complexation results in a decrease in the viscosity of polymer solutions.
36559750	7	87	theme	soil/sand	1308:1316	arg1	particles					1318:1326	soil/sand particles	1308:1326	soil/sand particles	1308:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	8	88	theme	e	1330:1330	arg1	polycomplexes					1342:1354	(e) Cationic polycomplexes	1329:1354	(e) Cationic polycomplexes	1329:1354	(e) Cationic polycomplexes form stronger coatings in comparison with anionic polycomplexes.
36559750	7	89	theme	%	1169:1169	arg1	solutions					1179:1187	1 wt% aqueous solutions	1165:1187	1 wt% aqueous solutions	1165:1187	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	1	90	theme	anionic	91:97	arg1	polysaccharide					99:112	A linear anionic polysaccharide	82:112	A linear anionic polysaccharide	82:112	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	1	90	theme	anionic	91:97	arg1	alginate					122:129	sodium alginate	115:129	sodium alginate	115:129	A linear anionic polysaccharide, sodium alginate, electrostatically interacts with a cationic polysaccharide, quaternized hydroxyethyl cellulose ethoxylate, in aqueous solution, thus giving an interpolyelectrolyte complex.
36559750	3	91	theme	following	723:731	arg1	conclusions					738:748	the following main conclusions	719:748	the following main conclusions	719:748	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	8	92	theme	Cationic	1333:1340	arg1	polycomplexes					1342:1354	(e) Cationic polycomplexes	1329:1354	(e) Cationic polycomplexes	1329:1354	(e) Cationic polycomplexes form stronger coatings in comparison with anionic polycomplexes.
36559750	2	93	theme	polymers	414:421	arg1	excess					380:385	an excess	377:385	an excess of the cationic or anionic polymers	377:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	0	94	theme	Anti-Erosion	37:48	arg1	Stabilization					50:62	Anti-Erosion Stabilization	37:62	Anti-Erosion Stabilization of Soil and Sand	37:79	Biodegradable Interpolycomplexes for Anti-Erosion Stabilization of Soil and Sand.
36559750	12	95	theme	small	1859:1863	arg1	fragments					1865:1873	small fragments	1859:1873	small fragments	1859:1873	(i) Films from polysaccharides and polycomplexes decompose down to small fragments after being in soil for 6 weeks.
36559750	6	96	theme	initial	1079:1085	arg1	components					1087:1096	the initial components	1075:1096	the initial components	1075:1096	(c) A complete dissociation of polycomplexes to the initial components is achieved in a 0.2 M NaCl solution.
36559750	6	97	dep	achieved	1101:1108	arg1	c					1028:1028	c	1028:1028	c	1028:1028	(c) A complete dissociation of polycomplexes to the initial components is achieved in a 0.2 M NaCl solution.
36559750	12	98	dep	Films	1796:1800	arg1	i					1793:1793	i	1793:1793	i	1793:1793	(i) Films from polysaccharides and polycomplexes decompose down to small fragments after being in soil for 6 weeks.
36559750	2	99	theme	anionic	406:412	arg1	polymers					414:421	the cationic or anionic polymers	390:421	the cationic or anionic polymers	390:421	Aqueous solutions of the initial polysaccharides and polycomplexes with an excess of the cationic or anionic polymers were used for the stabilization of soil and sand against water erosion.
36559750	4	100	theme	anionic	849:855	arg1	"					856:856	"anionic"	848:856	"anionic"	848:856	(a) Non-stoichiometric polycomplexes with an excess of cationic or anionic units ("cationic" and "anionic" polycomplexes, respectively) form transparent solutions or stable-in-time dispersions.
36559750	7	101	theme	composite	1269:1277	arg1	coatings					1279:1286	strong composite coatings	1262:1286	strong composite coatings from polymer(s) and soil/sand particles	1262:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	3	102	theme	main	733:736	arg1	conclusions					738:748	the following main conclusions	719:748	the following main conclusions	719:748	Physicochemical, mechanical and biological properties of the polymers and coatings were characterized by gravimetric analysis, viscosimetry, mechanical strength assessment, cell viability, and cell-mediated degradation with the following main conclusions.
36559750	13	103	theme	biodegradable	2001:2013	arg1	coatings					2026:2033	biodegradable protective coatings	2001:2033	biodegradable protective coatings	2001:2033	The results of the work are of importance for constructing water-resistant, low toxicity and biodegradable protective coatings for soil and sand.
36559750	7	104	from	s	1301:1301	arg1	formation					1249:1257	the formation	1245:1257	the formation of strong composite coatings from polymer(s) and soil/sand particles	1245:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	7	104	from	s	1301:1301	arg1	coatings					1279:1286	strong composite coatings	1262:1286	strong composite coatings from polymer(s) and soil/sand particles	1262:1326	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
36559750	5	105	theme	solutions	1016:1024	arg1	viscosity					995:1003	the viscosity	991:1003	the viscosity of polymer solutions	991:1024	(b) The complexation results in a decrease in the viscosity of polymer solutions.
36559750	8	106	theme	anionic	1398:1404	arg1	polycomplexes					1406:1418	anionic polycomplexes	1398:1418	anionic polycomplexes	1398:1418	(e) Cationic polycomplexes form stronger coatings in comparison with anionic polycomplexes.
36559750	6	107	theme	NaCl	1121:1124	arg1	solution					1126:1133	a 0.2 M NaCl solution	1113:1133	a 0.2 M NaCl solution	1113:1133	(c) A complete dissociation of polycomplexes to the initial components is achieved in a 0.2 M NaCl solution.
36559750	7	108	theme	aqueous	1171:1177	arg1	solutions					1179:1187	1 wt% aqueous solutions	1165:1187	1 wt% aqueous solutions	1165:1187	(d) Soil/sand treatment with 1 wt% aqueous solutions of polymers or polycomplexes and further drying lead to the formation of strong composite coatings from polymer(s) and soil/sand particles.
34503411	6	0	theme	MALDI-TOF	979:987	arg1	MS					989:990	MALDI-TOF MS	979:990	MALDI-TOF MS	979:990	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	4	1	dep	bacteria	573:580	arg1	functions					599:607	functions	599:607	functions	599:607	Furthermore, macromolecules are utilized to study bacteria's structures and functions.
34503411	4	1	dep	bacteria	573:580	arg1	structures					584:593	structures	584:593	structures	584:593	Furthermore, macromolecules are utilized to study bacteria's structures and functions.
34503411	2	2	dep	are	289:291	arg1	provide					408:414	provide	408:414	provide comprehensive information	408:440	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	2	2	dep	are	289:291	arg1	which					372:376	which	372:376	which	372:376	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	2	2	dep	are	289:291	arg1	methods					396:402	complementary methods	382:402	complementary methods	382:402	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	1	3	theme	essential	245:253	arg1	role					255:258	an essential role	242:258	an essential role	242:258	Bacteria build their structures by implementing several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis.
34503411	5	4	theme	biofilm	826:832	arg1	formation					834:842	biofilm formation	826:842	biofilm formation	826:842	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	0	5	from	Proteomics	10:19	arg1	Studies					57:63	Pathogenesis Studies	44:63	Pathogenesis Studies	44:63	Bacterial Proteomics and its Application in Pathogenesis Studies.
34503411	6	6	theme	valuable	1047:1054	arg1	tools					1056:1060	valuable tools	1047:1060	valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis	1047:1171	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	6	theme	valuable	1047:1054	arg1	electrophoresis					1020:1034	two-dimensional electrophoresis	1004:1034	two-dimensional electrophoresis	1004:1034	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	6	theme	valuable	1047:1054	arg1	devices					963:969	various devices	955:969	various devices	955:969	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	6	theme	valuable	1047:1054	arg1	LC-MS					993:997	LC-MS	993:997	LC-MS	993:997	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	6	theme	valuable	1047:1054	arg1	MS					989:990	MALDI-TOF MS	979:990	MALDI-TOF MS	979:990	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	5	7	theme	external	700:707	arg1	structures					709:718	the external structures	696:718	the external structures	696:718	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	8	theme	comprehensive	645:657	arg1	information					659:669	comprehensive information	645:669	comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment	645:900	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	6	9	theme	pathogenesis	1160:1171	arg1	proteins					1111:1118	different structural and functional proteins	1075:1118	different structural and functional proteins of the bacteria	1075:1134	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	9	theme	pathogenesis	1160:1171	arg1	mechanisms					1146:1155	their mechanisms	1140:1155	their mechanisms of pathogenesis	1140:1171	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	3	10	theme	cell	504:507	arg1	applications					509:520	their cell applications	498:520	their cell applications	498:520	Proteomic approaches are used to identify proteins and their cell applications.
34503411	6	11	theme	infections	1225:1234	arg1	diagnosis					1208:1216	rapid, easy, and accurate diagnosis	1182:1216	rapid, easy, and accurate diagnosis of the infections	1182:1234	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	5	12	theme	bacterial	913:921	arg1	pathogenesis					923:934	bacterial pathogenesis	913:934	bacterial pathogenesis	913:934	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	13	theme	particular	796:805	arg1	formation					834:842	biofilm formation	826:842	biofilm formation	826:842	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	13	theme	particular	796:805	arg1	resistance					856:865	antibiotic resistance	845:865	antibiotic resistance	845:865	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	13	theme	particular	796:805	arg1	adaptation					872:881	adaptation	872:881	adaptation to the environment	872:900	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	13	theme	particular	796:805	arg1	actions					807:813	particular actions	796:813	particular actions	796:813	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	2	14	theme	complementary	382:394	arg1	which					372:376	which	372:376	which	372:376	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	2	14	theme	complementary	382:394	arg1	methods					396:402	complementary methods	382:402	complementary methods	382:402	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	0	15	from	Application	29:39	arg1	Studies					57:63	Pathogenesis Studies	44:63	Pathogenesis Studies	44:63	Bacterial Proteomics and its Application in Pathogenesis Studies.
34503411	2	16	theme	proteomic	309:317	arg1	methods					319:325	two genomic and proteomic methods	293:325	two genomic and proteomic methods	293:325	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	6	17	theme	various	955:961	arg1	tools					1056:1060	valuable tools	1047:1060	valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis	1047:1171	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	17	theme	various	955:961	arg1	electrophoresis					1020:1034	two-dimensional electrophoresis	1004:1034	two-dimensional electrophoresis	1004:1034	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	17	theme	various	955:961	arg1	devices					963:969	various devices	955:969	various devices	955:969	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	17	theme	various	955:961	arg1	LC-MS					993:997	LC-MS	993:997	LC-MS	993:997	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	17	theme	various	955:961	arg1	MS					989:990	MALDI-TOF MS	979:990	MALDI-TOF MS	979:990	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	1	18	theme	nucleic	191:197	arg1	acids					199:203	nucleic acids	191:203	nucleic acids	191:203	Bacteria build their structures by implementing several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis.
34503411	0	19	theme	Bacterial	0:8	arg1	Proteomics					10:19	Bacterial Proteomics	0:19	Bacterial Proteomics	0:19	Bacterial Proteomics and its Application in Pathogenesis Studies.
34503411	4	20	used	utilized	555:562	arg2	macromolecules					536:549	macromolecules	536:549	macromolecules	536:549	Furthermore, macromolecules are utilized to study bacteria's structures and functions.
34503411	3	21	used	used	468:471	arg2	approaches					453:462	Proteomic approaches	443:462	Proteomic approaches	443:462	Proteomic approaches are used to identify proteins and their cell applications.
34503411	3	22	theme	Proteomic	443:451	arg1	approaches					453:462	Proteomic approaches	443:462	Proteomic approaches	443:462	Proteomic approaches are used to identify proteins and their cell applications.
34503411	5	23	theme	proteinbased	616:627	arg1	methods					629:635	These proteinbased methods	610:635	These proteinbased methods	610:635	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	2	24	theme	genomic	297:303	arg1	methods					319:325	two genomic and proteomic methods	293:325	two genomic and proteomic methods	293:325	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	5	25	theme	post-translational	744:761	arg1	modifications					763:775	post-translational modifications	744:775	post-translational modifications	744:775	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	1	26	theme	several	114:120	arg1	polysaccharides					155:169	polysaccharides	155:169	polysaccharides	155:169	Bacteria build their structures by implementing several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis.
34503411	1	26	theme	several	114:120	arg1	macromolecules					122:135	several macromolecules	114:135	several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis	114:280	Bacteria build their structures by implementing several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis.
34503411	1	26	theme	several	114:120	arg1	acids					199:203	nucleic acids	191:203	nucleic acids	191:203	Bacteria build their structures by implementing several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis.
34503411	1	26	theme	several	114:120	arg1	phospholipids					172:184	phospholipids	172:184	phospholipids	172:184	Bacteria build their structures by implementing several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis.
34503411	1	26	theme	several	114:120	arg1	proteins					145:152	proteins	145:152	proteins	145:152	Bacteria build their structures by implementing several macromolecules such as proteins, polysaccharides, phospholipids, and nucleic acids, which preserve their lives and play an essential role in their pathogenesis.
34503411	2	27	theme	comprehensive	416:428	arg1	information					430:440	comprehensive information	416:440	comprehensive information	416:440	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	6	28	theme	two-dimensional	1004:1018	arg1	electrophoresis					1020:1034	two-dimensional electrophoresis	1004:1034	two-dimensional electrophoresis	1004:1034	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	29	theme	bacteria	1127:1134	arg1	proteins					1111:1118	different structural and functional proteins	1075:1118	different structural and functional proteins of the bacteria	1075:1134	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	29	theme	bacteria	1127:1134	arg1	mechanisms					1146:1155	their mechanisms	1140:1155	their mechanisms of pathogenesis	1140:1171	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	30	theme	functional	1100:1109	arg1	proteins					1111:1118	different structural and functional proteins	1075:1118	different structural and functional proteins of the bacteria	1075:1134	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	5	31	theme	antibiotic	845:854	arg1	resistance					856:865	antibiotic resistance	845:865	antibiotic resistance	845:865	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	2	32	theme	various	336:342	arg1	macromolecules					344:357	various macromolecules	336:357	various macromolecules of bacteria	336:369	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
34503411	0	33	theme	Pathogenesis	44:55	arg1	Studies					57:63	Pathogenesis Studies	44:63	Pathogenesis Studies	44:63	Bacterial Proteomics and its Application in Pathogenesis Studies.
34503411	6	34	theme	easy	1189:1192	arg1	diagnosis					1208:1216	rapid, easy, and accurate diagnosis	1182:1216	rapid, easy, and accurate diagnosis of the infections	1182:1234	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	5	35	theme	internal	721:728	arg1	compositions					730:741	internal compositions	721:741	internal compositions	721:741	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	6	36	theme	accurate	1199:1206	arg1	diagnosis					1208:1216	rapid, easy, and accurate diagnosis	1182:1216	rapid, easy, and accurate diagnosis of the infections	1182:1234	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	37	theme	rapid	1182:1186	arg1	diagnosis					1208:1216	rapid, easy, and accurate diagnosis	1182:1216	rapid, easy, and accurate diagnosis of the infections	1182:1234	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	6	38	theme	structural	1085:1094	arg1	proteins					1111:1118	different structural and functional proteins	1075:1118	different structural and functional proteins of the bacteria	1075:1134	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	5	39	theme	actions	807:813	arg1	structures					709:718	the external structures	696:718	the external structures	696:718	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	39	theme	actions	807:813	arg1	compositions					730:741	internal compositions	721:741	internal compositions	721:741	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	39	theme	actions	807:813	arg1	modifications					763:775	post-translational modifications	744:775	post-translational modifications	744:775	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	5	39	theme	actions	807:813	arg1	mechanisms					782:791	mechanisms	782:791	mechanisms	782:791	These proteinbased methods provide comprehensive information about the cells, such as the external structures, internal compositions, post-translational modifications, and mechanisms of particular actions, including biofilm formation, antibiotic resistance, and adaptation to the environment, promoting bacterial pathogenesis.
34503411	6	40	theme	different	1075:1083	arg1	proteins					1111:1118	different structural and functional proteins	1075:1118	different structural and functional proteins of the bacteria	1075:1134	These methods use various devices such as MALDI-TOF MS, LC-MS, and two-dimensional electrophoresis, which are valuable tools for studying different structural and functional proteins of the bacteria and their mechanisms of pathogenesis, causing rapid, easy, and accurate diagnosis of the infections.
34503411	2	41	theme	bacteria	362:369	arg1	macromolecules					344:357	various macromolecules	336:357	various macromolecules of bacteria	336:369	There are two genomic and proteomic methods to study various macromolecules of bacteria, which are complementary methods and provide comprehensive information.
37090772	3	0	theme	fruit	461:465	arg1	polysaccharides					467:481	six mulberry fruit polysaccharides	448:481	six mulberry fruit polysaccharides (MFPs)	448:488	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	3	0	theme	fruit	461:465	arg1	MFPs					484:487	MFPs	484:487	MFPs	484:487	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	7	1	theme	aforementioned	1435:1448	arg1	research					1450:1457	the aforementioned research	1431:1457	the aforementioned research	1431:1457	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	6	2	theme	thermal	1335:1341	arg1	stability					1343:1351	thermal stability	1335:1351	thermal stability	1335:1351	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	0	3	from	characteristics	11:25	arg1	Fructus					97:103	Fructus	97:103	Fructus	97:103	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	2	4	theme	pharmacological	319:333	arg1	components					335:344	important pharmacological components	309:344	important pharmacological components of mulberry	309:356	Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity.
37090772	8	5	theme	ideal	1720:1724	arg1	activity					1737:1744	ideal biological activity	1720:1744	ideal biological activity	1720:1744	This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
37090772	7	6	theme	mulberry	1564:1571	arg1	polysaccharides					1573:1587	mulberry polysaccharides	1564:1587	mulberry polysaccharides	1564:1587	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	6	7	theme	thermogravimetric	1188:1204	arg1	TG					1216:1217	TG	1216:1217	TG	1216:1217	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	7	theme	thermogravimetric	1188:1204	arg1	analysis					1206:1213	thermogravimetric analysis	1188:1213	thermogravimetric analysis (TG)	1188:1218	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	5	8	theme	apparent	939:946	arg1	morphology					948:957	apparent morphology	939:957	apparent morphology	939:957	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	7	9	theme	biological	1541:1550	arg1	activity					1552:1559	biological activity	1541:1559	biological activity	1541:1559	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	8	10	theme	scientific	1621:1630	arg1	basis					1632:1636	a scientific basis	1619:1636	a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity	1619:1744	This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
37090772	7	11	theme	chemical	1516:1523	arg1	composition					1525:1535	chemical composition	1516:1535	chemical composition	1516:1535	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	5	12	theme	liquid	885:890	arg1	HPLC					908:911	HPLC	908:911	HPLC	908:911	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	5	12	theme	liquid	885:890	arg1	chromatography					892:905	high-performance liquid chromatography	868:905	high-performance liquid chromatography (HPLC) analysis	868:921	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	0	13	theme	extraction	124:133	arg1	methods					135:141	different extraction methods	114:141	different extraction methods	114:141	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	0	14	from	activities	57:66	arg1	Fructus					97:103	Fructus	97:103	Fructus	97:103	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	6	15	theme	red	1235:1237	arg1	experiment					1239:1248	the Congo red experiment	1225:1248	the Congo red experiment	1225:1248	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	2	16	theme	study	382:386	arg1	study					382:386	study	382:386	study	382:386	Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity.
37090772	2	16	theme	study	382:386	arg1	lot					375:377	a lot	373:377	a lot of study for their structure and biological activity	373:430	Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity.
37090772	5	17	theme	monosaccharide	963:976	arg1	content					978:984	monosaccharide content	963:984	monosaccharide content	963:984	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	8	18	theme	biological	1726:1735	arg1	activity					1737:1744	ideal biological activity	1720:1744	ideal biological activity	1720:1744	This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
37090772	0	19	theme	different	114:122	arg1	methods					135:141	different extraction methods	114:141	different extraction methods	114:141	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	5	20	theme	chromatography	892:905	arg1	analysis					914:921	high-performance liquid chromatography (HPLC) analysis	868:921	high-performance liquid chromatography (HPLC) analysis	868:921	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	6	21	theme	helix	1314:1318	arg1	structure					1320:1328	triple helix structure	1307:1328	triple helix structure	1307:1328	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	22	theme	magnetic	1162:1169	arg1	resonance					1171:1179	nuclear magnetic resonance	1154:1179	nuclear magnetic resonance (NMR)	1154:1185	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	22	theme	magnetic	1162:1169	arg1	NMR					1182:1184	NMR	1182:1184	NMR	1182:1184	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	23	theme	experiments	1076:1086	arg1	results					1065:1071	The results	1061:1071	The results of experiments	1061:1086	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	2	24	theme	biological	412:421	arg1	activity					423:430	biological activity	412:430	biological activity	412:430	Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity.
37090772	6	25	theme	nuclear	1154:1160	arg1	resonance					1171:1179	nuclear magnetic resonance	1154:1179	nuclear magnetic resonance (NMR)	1154:1185	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	25	theme	nuclear	1154:1160	arg1	NMR					1182:1184	NMR	1182:1184	NMR	1182:1184	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	7	26	theme	polysaccharides	1573:1587	arg1	composition					1525:1535	chemical composition	1516:1535	chemical composition	1516:1535	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	7	26	theme	polysaccharides	1573:1587	arg1	activity					1552:1559	biological activity	1541:1559	biological activity	1541:1559	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	0	27	theme	Structural	0:9	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	4	28	theme	best	709:712	arg1	α-glucosidase					714:726	α-glucosidase	714:726	α-glucosidase	714:726	According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS.
37090772	6	29	theme	triple	1307:1312	arg1	structure					1320:1328	triple helix structure	1307:1328	triple helix structure	1307:1328	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	3	30	theme	different	508:516	arg1	methods					529:535	different extraction methods	508:535	different extraction methods	508:535	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	6	31	theme	extraction	1398:1407	arg1	methods					1409:1415	the extraction methods	1394:1415	the extraction methods	1394:1415	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	0	32	from	Fructus	97:103	arg1	polysaccharides					71:85	polysaccharides	71:85	polysaccharides from Mori Fructus	71:103	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	0	32	from	Fructus	97:103	arg1	antioxidant					28:38	antioxidant	28:38	antioxidant	28:38	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	0	32	from	Fructus	97:103	arg1	activities					57:66	hypoglycemic activities	44:66	hypoglycemic activities	44:66	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	0	32	from	Fructus	97:103	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	3	33	theme	extraction	518:527	arg1	methods					529:535	different extraction methods	508:535	different extraction methods	508:535	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	8	34	theme	mulberry	1690:1697	arg1	polysaccharides					1699:1713	mulberry polysaccharides	1690:1713	mulberry polysaccharides with ideal biological activity	1690:1744	This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
37090772	1	35	theme	many	190:193	arg1	nutrients					195:203	many nutrients	190:203	many nutrients needed by the human body	190:228	The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine.
37090772	0	36	theme	hypoglycemic	44:55	arg1	activities					57:66	hypoglycemic activities	44:66	hypoglycemic activities	44:66	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	8	37	theme	extraction	1661:1670	arg1	methods					1672:1678	suitable extraction methods	1652:1678	suitable extraction methods	1652:1678	This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
37090772	3	38	theme	physicochemical	548:562	arg1	structures					564:573	their physicochemical structures	542:573	their physicochemical structures	542:573	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	5	39	theme	Scanner	830:836	arg1	SEM					859:861	SEM	859:861	SEM	859:861	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	5	39	theme	Scanner	830:836	arg1	microscopy					847:856	Scanner electron microscopy	830:856	Scanner electron microscopy (SEM)	830:862	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	6	40	theme	Congo	1229:1233	arg1	experiment					1239:1248	the Congo red experiment	1225:1248	the Congo red experiment	1225:1248	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	7	41	dep	composition	1525:1535	arg1	the					1512:1514	the	1512:1514	the	1512:1514	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	6	42	theme	glycosidic	1289:1298	arg1	bonds					1300:1304	glycosidic bonds	1289:1304	glycosidic bonds	1289:1304	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	3	43	theme	hypoglycemic	593:604	arg1	activities					617:626	hypoglycemic biological activities	593:626	hypoglycemic biological activities	593:626	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	4	44	contain	had	769:771	arg2	activity					798:805	the strongest scavenging activity	773:805	the strongest scavenging activity	773:805	According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS.
37090772	4	44	contain	had	769:771	arg1	MFP-IV					762:767	MFP-IV	762:767	MFP-IV	762:767	According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS.
37090772	1	45	from	nutrients	195:203	arg1	rich					182:185	rich	182:185	rich	182:185	The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine.
37090772	1	45	from	nutrients	195:203	arg1	mulberry					148:155	The mulberry	144:155	The mulberry (Mori Fructus)	144:170	The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine.
37090772	5	46	theme	electron	838:845	arg1	SEM					859:861	SEM	859:861	SEM	859:861	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	5	46	theme	electron	838:845	arg1	microscopy					847:856	Scanner electron microscopy	830:856	Scanner electron microscopy (SEM)	830:862	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	3	47	theme	biological	606:615	arg1	activities					617:626	hypoglycemic biological activities	593:626	hypoglycemic biological activities	593:626	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	6	48	dep	Fourier	1105:1111	arg1	transform					1113:1121	transform	1113:1121	transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment	1113:1248	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	8	49	with	polysaccharides	1699:1713	arg1	activity					1737:1744	ideal biological activity	1720:1744	ideal biological activity	1720:1744	This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
37090772	2	50	theme	important	309:317	arg1	components					335:344	important pharmacological components	309:344	important pharmacological components of mulberry	309:356	Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity.
37090772	7	51	theme	various	1460:1466	arg1	methods					1479:1485	various extraction methods	1460:1485	various extraction methods	1460:1485	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	7	52	theme	extraction	1468:1477	arg1	methods					1479:1485	various extraction methods	1460:1485	various extraction methods	1460:1485	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	5	53	theme	extraction	1038:1047	arg1	techniques					1049:1058	the different extraction techniques	1024:1058	the different extraction techniques	1024:1058	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	5	54	theme	different	1028:1036	arg1	techniques					1049:1058	the different extraction techniques	1024:1058	the different extraction techniques	1024:1058	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	3	55	theme	mulberry	452:459	arg1	polysaccharides					467:481	six mulberry fruit polysaccharides	448:481	six mulberry fruit polysaccharides (MFPs)	448:488	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	3	55	theme	mulberry	452:459	arg1	MFPs					484:487	MFPs	484:487	MFPs	484:487	In this study, six mulberry fruit polysaccharides (MFPs) were extracted by different extraction methods, and their physicochemical structures, antioxidant, and hypoglycemic biological activities were investigated and compared.
37090772	6	56	theme	functional	1270:1279	arg1	groups					1281:1286	the MFP functional groups	1262:1286	the MFP functional groups	1262:1286	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	56	theme	functional	1270:1279	arg1	different					1376:1384	different	1376:1384	different	1376:1384	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	5	57	dep	morphology	948:957	arg1	the					935:937	the	935:937	the	935:937	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	4	58	theme	α-amylase	732:740	arg1	inhibition					742:751	α-amylase inhibition	732:751	α-amylase inhibition	732:751	According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS.
37090772	6	59	theme	MFP	1266:1268	arg1	groups					1281:1286	the MFP functional groups	1262:1286	the MFP functional groups	1262:1286	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	59	theme	MFP	1266:1268	arg1	different					1376:1384	different	1376:1384	different	1376:1384	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	5	60	theme	MFP	989:991	arg1	morphology					948:957	apparent morphology	939:957	apparent morphology	939:957	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	5	60	theme	MFP	989:991	arg1	content					978:984	monosaccharide content	963:984	monosaccharide content	963:984	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	6	61	theme	infrared	1123:1130	arg1	FT-IR					1146:1150	FT-IR	1146:1150	FT-IR	1146:1150	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	6	61	theme	infrared	1123:1130	arg1	spectroscopy					1132:1143	infrared spectroscopy	1123:1143	infrared spectroscopy (FT-IR)	1123:1151	The results of experiments using Congo red, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), thermogravimetric analysis (TG), and the Congo red experiment showed that the MFP functional groups, glycosidic bonds, triple helix structure, and thermal stability were not significantly different between the extraction methods.
37090772	7	62	theme	different	1491:1499	arg1	effects					1501:1507	different effects	1491:1507	different effects	1491:1507	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	4	63	theme	scavenging	787:796	arg1	activity					798:805	the strongest scavenging activity	773:805	the strongest scavenging activity	773:805	According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS.
37090772	1	64	from	rich	182:185	arg1	nutrients					195:203	many nutrients	190:203	many nutrients needed by the human body	190:228	The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine.
37090772	2	65	theme	mulberry	349:356	arg1	components					335:344	important pharmacological components	309:344	important pharmacological components of mulberry	309:356	Polysaccharides, which are considered to be important pharmacological components of mulberry, have received a lot of study for their structure and biological activity.
37090772	7	66	contain	had	1487:1489	arg2	effects					1501:1507	different effects	1491:1507	different effects	1491:1507	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	7	66	contain	had	1487:1489	arg1	methods					1479:1485	various extraction methods	1460:1485	various extraction methods	1460:1485	According to the aforementioned research, various extraction methods had different effects on the chemical composition and biological activity of mulberry polysaccharides.
37090772	1	67	theme	human	219:223	arg1	body					225:228	the human body	215:228	the human body	215:228	The mulberry (Mori Fructus), which is rich in many nutrients needed by the human body, serves as both food and medicine.
37090772	0	68	theme	polysaccharides	71:85	arg1	antioxidant					28:38	antioxidant	28:38	antioxidant	28:38	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	0	68	theme	polysaccharides	71:85	arg1	activities					57:66	hypoglycemic activities	44:66	hypoglycemic activities	44:66	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	0	68	theme	polysaccharides	71:85	arg1	characteristics					11:25	Structural characteristics	0:25	Structural characteristics	0:25	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	0	69	from	antioxidant	28:38	arg1	Fructus					97:103	Fructus	97:103	Fructus	97:103	Structural characteristics, antioxidant and hypoglycemic activities of polysaccharides from Mori Fructus based on different extraction methods.
37090772	4	70	theme	strongest	777:785	arg1	activity					798:805	the strongest scavenging activity	773:805	the strongest scavenging activity	773:805	According to the findings, MFP-III exhibited the best α-glucosidase and α-amylase inhibition, whereas MFP-IV had the strongest scavenging activity against DPPH and ABTS.
37090772	5	71	theme	high-performance	868:883	arg1	HPLC					908:911	HPLC	908:911	HPLC	908:911	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	5	71	theme	high-performance	868:883	arg1	chromatography					892:905	high-performance liquid chromatography	868:905	high-performance liquid chromatography (HPLC) analysis	868:921	Scanner electron microscopy (SEM) and high-performance liquid chromatography (HPLC) analysis showed that the apparent morphology and monosaccharide content of MFP were significantly impacted by the different extraction techniques.
37090772	8	72	theme	suitable	1652:1659	arg1	methods					1672:1678	suitable extraction methods	1652:1678	suitable extraction methods	1652:1678	This information can provide a scientific basis for selecting suitable extraction methods to obtain mulberry polysaccharides with ideal biological activity.
35519816	0	0	theme	Marine	85:90	arg1	Sediments					92:100	Marine Sediments	85:100	Marine Sediments	85:100	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	0	1	from	Preservation	14:25	arg1	Sediments					92:100	Marine Sediments	85:100	Marine Sediments	85:100	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	3	2	theme	high-throughput	462:476	arg1	profiling					493:501	high-throughput polysaccharide profiling	462:501	high-throughput polysaccharide profiling	462:501	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	1	3	theme	compounds	240:248	arg1	degradation					219:229	degradation	219:229	degradation	219:229	Polysaccharides constitute an important carbon pool in marine systems, but much is still unknown about the fate and degradation of these compounds.
35519816	1	3	theme	compounds	240:248	arg1	fate					210:213	fate	210:213	fate	210:213	Polysaccharides constitute an important carbon pool in marine systems, but much is still unknown about the fate and degradation of these compounds.
35519816	4	4	theme	compounds	871:879	arg1	compounds					744:752	Preserved compounds	734:752	Preserved compounds	734:752	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	4	theme	compounds	871:879	arg1	remains					758:764	remains	758:764	remains	758:764	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	4	theme	compounds	871:879	arg1	composition					846:856	composition	846:856	composition	846:856	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	4	theme	compounds	871:879	arg1	compounds					871:879	different compounds	861:879	different compounds that can provide insight into past environmental conditions	861:939	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	4	theme	compounds	871:879	arg1	amount					835:840	amount	835:840	amount	835:840	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	3	5	theme	polysaccharide	478:491	arg1	profiling					493:501	high-throughput polysaccharide profiling	462:501	high-throughput polysaccharide profiling	462:501	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	3	6	theme	algal	517:521	arg1	compounds					533:541	algal cell-wall compounds	517:541	algal cell-wall compounds	517:541	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	4	7	theme	Preserved	734:742	arg1	composition					846:856	composition	846:856	composition	846:856	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	7	theme	Preserved	734:742	arg1	remains					758:764	remains	758:764	remains	758:764	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	7	theme	Preserved	734:742	arg1	proxies					820:826	paleoenvironmental proxies	801:826	paleoenvironmental proxies	801:826	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	7	theme	Preserved	734:742	arg1	compounds					871:879	different compounds	861:879	different compounds that can provide insight into past environmental conditions	861:939	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	7	theme	Preserved	734:742	arg1	amount					835:840	amount	835:840	amount	835:840	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	7	theme	Preserved	734:742	arg1	compounds					744:752	Preserved compounds	734:752	Preserved compounds	734:752	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	3	8	theme	environmental	711:723	arg1	changes					725:731	temporal environmental changes	702:731	temporal environmental changes	702:731	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	3	9	theme	cell-wall	523:531	arg1	compounds					533:541	algal cell-wall compounds	517:541	algal cell-wall compounds	517:541	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	4	10	theme	environmental	972:984	arg1	changes					986:992	environmental changes	972:992	environmental changes	972:992	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	3	11	from	environment	589:599	arg1	cores					561:565	dated sediment cores	546:565	dated sediment cores from a coastal marine environment	546:599	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	0	12	from	Fluctuations	40:51	arg1	Sediments					92:100	Marine Sediments	85:100	Marine Sediments	85:100	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	0	13	theme	100-Year	5:12	arg1	Preservation					14:25	100-Year Preservation	5:25	100-Year Preservation	5:25	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	3	14	theme	temporal	702:709	arg1	changes					725:731	temporal environmental changes	702:731	temporal environmental changes	702:731	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	4	15	theme	different	861:869	arg1	compounds					871:879	different compounds	861:879	different compounds that can provide insight into past environmental conditions	861:939	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	1	16	from	pool	150:153	arg1	systems					165:171	marine systems	158:171	marine systems	158:171	Polysaccharides constitute an important carbon pool in marine systems, but much is still unknown about the fate and degradation of these compounds.
35519816	3	17	theme	dated	546:550	arg1	cores					561:565	dated sediment cores	546:565	dated sediment cores from a coastal marine environment	546:599	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	3	18	theme	sediment	552:559	arg1	cores					561:565	dated sediment cores	546:565	dated sediment cores from a coastal marine environment	546:599	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	4	19	theme	paleoenvironmental	801:818	arg1	compounds					744:752	Preserved compounds	734:752	Preserved compounds	734:752	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	19	theme	paleoenvironmental	801:818	arg1	remains					758:764	remains	758:764	remains	758:764	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	19	theme	paleoenvironmental	801:818	arg1	proxies					820:826	paleoenvironmental proxies	801:826	paleoenvironmental proxies	801:826	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	20	dep	amount	835:840	arg1	the					831:833	the	831:833	the	831:833	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	2	21	theme	in	291:292	arg1	production					280:289	production	280:289	production in situ	280:297	They are derived partly from production in situ, and in coastal areas, they are partly terrestrially derived, originating from freshwater runoff from land.
35519816	1	22	theme	important	133:141	arg1	pool					150:153	an important carbon pool	130:153	an important carbon pool in marine systems	130:171	Polysaccharides constitute an important carbon pool in marine systems, but much is still unknown about the fate and degradation of these compounds.
35519816	4	23	theme	novel	946:950	arg1	means					952:956	novel means	946:956	novel means for reporting environmental changes	946:992	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	24	used	used	793:796	arg2	amount					835:840	amount	835:840	amount	835:840	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	24	used	used	793:796	arg2	compounds					871:879	different compounds	861:879	different compounds that can provide insight into past environmental conditions	861:939	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	24	used	used	793:796	arg2	compounds					744:752	Preserved compounds	734:752	Preserved compounds	734:752	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	24	used	used	793:796	arg2	remains					758:764	remains	758:764	remains	758:764	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	24	used	used	793:796	arg2	composition					846:856	composition	846:856	composition	846:856	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	4	24	used	used	793:796	arg2	proxies					820:826	paleoenvironmental proxies	801:826	paleoenvironmental proxies	801:826	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	2	25	theme	runoff	389:394	arg1	freshwater					378:387	freshwater runoff	378:394	freshwater runoff	378:394	They are derived partly from production in situ, and in coastal areas, they are partly terrestrially derived, originating from freshwater runoff from land.
35519816	0	26	theme	Temporal	31:38	arg1	Fluctuations					40:51	Temporal Fluctuations	31:51	Temporal Fluctuations	31:51	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	3	27	theme	coastal	574:580	arg1	environment					589:599	a coastal marine environment	572:599	a coastal marine environment	572:599	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	3	28	theme	study	423:427	arg1	aim					411:413	The aim	407:413	The aim of this study	407:427	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	0	29	theme	Cell	56:59	arg1	Polysaccharides					66:80	Cell Wall Polysaccharides	56:80	Cell Wall Polysaccharides	56:80	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	2	30	attach	derived	260:266	arg1	production					280:289	production	280:289	production in situ	280:297	They are derived partly from production in situ, and in coastal areas, they are partly terrestrially derived, originating from freshwater runoff from land.
35519816	2	30	attach	derived	260:266	arg2	They					251:254	They	251:254	They	251:254	They are derived partly from production in situ, and in coastal areas, they are partly terrestrially derived, originating from freshwater runoff from land.
35519816	2	31	dep	in	291:292	arg1	situ					294:297	situ	294:297	situ	294:297	They are derived partly from production in situ, and in coastal areas, they are partly terrestrially derived, originating from freshwater runoff from land.
35519816	4	32	theme	environmental	916:928	arg1	conditions					930:939	past environmental conditions	911:939	past environmental conditions	911:939	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	1	33	theme	carbon	143:148	arg1	pool					150:153	an important carbon pool	130:153	an important carbon pool in marine systems	130:171	Polysaccharides constitute an important carbon pool in marine systems, but much is still unknown about the fate and degradation of these compounds.
35519816	4	34	theme	past	911:914	arg1	conditions					930:939	past environmental conditions	911:939	past environmental conditions	911:939	Preserved compounds and remains of organisms are routinely used as paleoenvironmental proxies as the amount and composition of different compounds that can provide insight into past environmental conditions, and novel means for reporting environmental changes are highly sought.
35519816	3	35	theme	cell-wall	633:641	arg1	polysaccharides					643:657	cell-wall polysaccharides	633:657	cell-wall polysaccharides	633:657	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	1	36	dep	fate	210:213	arg1	the					206:208	the	206:208	the	206:208	Polysaccharides constitute an important carbon pool in marine systems, but much is still unknown about the fate and degradation of these compounds.
35519816	2	37	theme	coastal	307:313	arg1	areas					315:319	coastal areas	307:319	coastal areas	307:319	They are derived partly from production in situ, and in coastal areas, they are partly terrestrially derived, originating from freshwater runoff from land.
35519816	0	38	theme	Polysaccharides	66:80	arg1	Preservation					14:25	100-Year Preservation	5:25	100-Year Preservation	5:25	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	0	38	theme	Polysaccharides	66:80	arg1	Fluctuations					40:51	Temporal Fluctuations	31:51	Temporal Fluctuations	31:51	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	3	39	theme	polysaccharides	643:657	arg1	preservation					617:628	the preservation	613:628	the preservation of cell-wall polysaccharides	613:657	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	3	40	theme	marine	582:587	arg1	environment					589:599	a coastal marine environment	572:599	a coastal marine environment	572:599	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	3	41	theme	profiling	493:501	arg1	applicability					445:457	the applicability	441:457	the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds	441:541	The aim of this study was to test the applicability of high-throughput polysaccharide profiling for plant and algal cell-wall compounds in dated sediment cores from a coastal marine environment, to examine the preservation of cell-wall polysaccharides and explore their potential as proxies for temporal environmental changes.
35519816	0	42	theme	Wall	61:64	arg1	Polysaccharides					66:80	Cell Wall Polysaccharides	56:80	Cell Wall Polysaccharides	56:80	Over 100-Year Preservation and Temporal Fluctuations of Cell Wall Polysaccharides in Marine Sediments.
35519816	1	43	theme	marine	158:163	arg1	systems					165:171	marine systems	158:171	marine systems	158:171	Polysaccharides constitute an important carbon pool in marine systems, but much is still unknown about the fate and degradation of these compounds.
37364300	5	0	theme	particle	1261:1268	arg1	size					1270:1273	the same particle size	1252:1273	the same particle size	1252:1273	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	6	1	theme	scanning	1336:1343	arg1	microscopy					1354:1363	scanning electron microscopy	1336:1363	scanning electron microscopy	1336:1363	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	6	2	theme	electron	1345:1352	arg1	microscopy					1354:1363	scanning electron microscopy	1336:1363	scanning electron microscopy	1336:1363	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	6	3	theme	harmful	1419:1425	arg1	pathways					1427:1434	the main harmful pathways	1410:1434	the main harmful pathways of different microplastics to algal	1410:1470	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	6	3	theme	harmful	1419:1425	arg1	effects					1397:1403	the adsorption effects	1382:1403	the adsorption effects	1382:1403	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	7	4	theme	harmful	1527:1533	arg1	effects					1535:1541	limited harmful effects	1519:1541	limited harmful effects	1519:1541	Our results suggested that microplastics have limited harmful effects on algae, mainly in adsorption and shading.
37364300	6	5	from	colonization	1292:1303	arg1	microplastics					1308:1320	microplastics	1308:1320	microplastics	1308:1320	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	0	6	theme	nutrient	70:77	arg1	composition					79:89	nutrient composition	70:89	nutrient composition	70:89	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	3	7	theme	intracellular	698:710	arg1	polysaccharides					712:726	soluble intracellular polysaccharides	690:726	soluble intracellular polysaccharides	690:726	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	5	8	dep	control	1229:1235	arg1	the					1225:1227	the	1225:1227	the	1225:1227	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	0	9	theme	freshwater	94:103	arg1	Beij					130:133	freshwater algae Chlorella vulgaris Beij	94:133	freshwater algae Chlorella vulgaris Beij	94:133	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	0	10	from	Effects	0:6	arg1	growth					32:37	growth	32:37	growth	32:37	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	0	10	from	Effects	0:6	arg1	efficiency					55:64	photosynthetic efficiency	40:64	photosynthetic efficiency	40:64	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	0	10	from	Effects	0:6	arg1	composition					79:89	nutrient composition	70:89	nutrient composition	70:89	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	3	11	theme	PE-MP	898:902	arg1	exposure					904:911	PE-MP exposure	898:911	PE-MP exposure	898:911	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	4	12	theme	TSP	995:997	arg1	contents					999:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	2	13	theme	algae	544:548	arg1	Beij					569:572	freshwater algae Chlorella vulgaris Beij	533:572	freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days	533:598	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	5	14	theme	photosynthetic	1128:1141	arg1	pigment					1143:1149	photosynthetic pigment	1128:1149	photosynthetic pigment	1128:1149	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	6	15	dep	C.	1280:1281	arg1	vulgaris					1283:1290	vulgaris	1283:1290	vulgaris	1283:1290	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	1	16	theme	microplastics	268:280	arg1	hazards					257:263	the hazards	253:263	the hazards of microplastics with different particle sizes, concentrations and materials	253:340	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	2	17	theme	freshwater	533:542	arg1	Beij					569:572	freshwater algae Chlorella vulgaris Beij	533:572	freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days	533:598	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	18	with	microplastics	420:432	arg1	concentrations					458:471	different concentrations	448:471	different concentrations	448:471	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	19	theme	polyethylene	407:418	arg1	PE-MPs					435:440	PE-MPs	435:440	PE-MPs	435:440	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	19	theme	polyethylene	407:418	arg1	microplastics					420:432	polyethylene microplastics	407:432	polyethylene microplastics (PE-MPs) with different concentrations	407:471	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	0	20	theme	algae	105:109	arg1	Beij					130:133	freshwater algae Chlorella vulgaris Beij	94:133	freshwater algae Chlorella vulgaris Beij	94:133	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	0	21	from	growth	32:37	arg1	Beij					130:133	freshwater algae Chlorella vulgaris Beij	94:133	freshwater algae Chlorella vulgaris Beij	94:133	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	5	22	theme	pigment	1143:1149	arg1	contents					1151:1158	the colony formation and photosynthetic pigment contents	1103:1158	the colony formation and photosynthetic pigment contents	1103:1158	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	1	23	theme	smaller	183:189	arg1	fragments					173:181	fragments	173:181	fragments smaller than 5 mm	173:199	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	4	24	theme	soluble	953:959	arg1	polysaccharides					975:989	soluble intracellular polysaccharides	953:989	soluble intracellular polysaccharides	953:989	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	7	25	theme	limited	1519:1525	arg1	effects					1535:1541	limited harmful effects	1519:1541	limited harmful effects	1519:1541	Our results suggested that microplastics have limited harmful effects on algae, mainly in adsorption and shading.
37364300	5	26	with	PE-MPs	1240:1245	arg1	size					1270:1273	the same particle size	1252:1273	the same particle size	1252:1273	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	3	27	theme	photosynthetic	757:770	arg1	efficiency					772:781	the photosynthetic efficiency	753:781	the photosynthetic efficiency	753:781	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	4	28	theme	particle	1064:1071	arg1	sizes					1073:1077	different particle sizes	1054:1077	different particle sizes	1054:1077	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	4	29	with	PE-MPs	1042:1047	arg1	sizes					1073:1077	different particle sizes	1054:1077	different particle sizes	1054:1077	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	4	29	with	PE-MPs	1042:1047	arg1	PS-MPs					1082:1087	PS-MPs	1082:1087	PS-MPs	1082:1087	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	6	30	theme	adsorption	1386:1395	arg1	pathways					1427:1434	the main harmful pathways	1410:1434	the main harmful pathways of different microplastics to algal	1410:1470	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	6	30	theme	adsorption	1386:1395	arg1	effects					1397:1403	the adsorption effects	1382:1403	the adsorption effects	1382:1403	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	5	31	theme	colony	1107:1112	arg1	formation					1114:1122	colony formation	1107:1122	colony formation	1107:1122	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	2	32	theme	different	448:456	arg1	concentrations					458:471	different concentrations	448:471	different concentrations	448:471	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	1	33	theme	different	287:295	arg1	sizes					306:310	different particle sizes	287:310	different particle sizes	287:310	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	5	34	with	control	1229:1235	arg1	size					1270:1273	the same particle size	1252:1273	the same particle size	1252:1273	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	6	35	theme	C.	1280:1281	arg1	colonization					1292:1303	The C. vulgaris colonization	1276:1303	The C. vulgaris colonization on microplastics	1276:1320	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	0	36	theme	microplastics	11:23	arg1	Effects					0:6	Effects	0:6	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.	0:134	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	1	37	theme	particle	297:304	arg1	sizes					306:310	different particle sizes	287:310	different particle sizes	287:310	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	0	38	theme	vulgaris	121:128	arg1	Beij					130:133	freshwater algae Chlorella vulgaris Beij	94:133	freshwater algae Chlorella vulgaris Beij	94:133	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	0	39	from	composition	79:89	arg1	Beij					130:133	freshwater algae Chlorella vulgaris Beij	94:133	freshwater algae Chlorella vulgaris Beij	94:133	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	2	40	theme	polystyrene	495:505	arg1	microplastics					507:519	polystyrene microplastics	495:519	polystyrene microplastics	495:519	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	7	41	contain	have	1514:1517	arg2	effects					1535:1541	limited harmful effects	1519:1541	limited harmful effects	1519:1541	Our results suggested that microplastics have limited harmful effects on algae, mainly in adsorption and shading.
37364300	7	41	contain	have	1514:1517	arg1	microplastics					1500:1512	microplastics	1500:1512	microplastics	1500:1512	Our results suggested that microplastics have limited harmful effects on algae, mainly in adsorption and shading.
37364300	4	42	theme	intracellular	961:973	arg1	polysaccharides					975:989	soluble intracellular polysaccharides	953:989	soluble intracellular polysaccharides	953:989	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	3	43	theme	photosynthetic	654:667	arg1	contents					677:684	photosynthetic pigment contents	654:684	photosynthetic pigment contents	654:684	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	0	44	theme	Chlorella	111:119	arg1	Beij					130:133	freshwater algae Chlorella vulgaris Beij	94:133	freshwater algae Chlorella vulgaris Beij	94:133	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	3	45	theme	total	791:795	arg1	protein					805:811	the total soluble protein	787:811	the total soluble protein (TSP) contents	787:826	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	3	45	theme	total	791:795	arg1	TSP					814:816	TSP	814:816	TSP	814:816	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	6	46	theme	different	1439:1447	arg1	microplastics					1449:1461	different microplastics	1439:1461	different microplastics	1439:1461	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	2	47	theme	vulgaris	560:567	arg1	Beij					569:572	freshwater algae Chlorella vulgaris Beij	533:572	freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days	533:598	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	3	48	theme	colony	636:641	arg1	formation					643:651	colony formation	636:651	colony formation	636:651	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	1	49	theme	various	219:225	arg1	environments					235:246	various aquatic environments	219:246	various aquatic environments	219:246	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	0	50	theme	photosynthetic	40:53	arg1	efficiency					55:64	photosynthetic efficiency	40:64	photosynthetic efficiency	40:64	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	2	51	theme	Chlorella	550:558	arg1	Beij					569:572	freshwater algae Chlorella vulgaris Beij	533:572	freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days	533:598	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	4	52	theme	growth	918:923	arg1	contents					999:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	0	53	from	efficiency	55:64	arg1	Beij					130:133	freshwater algae Chlorella vulgaris Beij	94:133	freshwater algae Chlorella vulgaris Beij	94:133	Effects of microplastics on the growth, photosynthetic efficiency and nutrient composition in freshwater algae Chlorella vulgaris Beij.
37364300	1	54	with	microplastics	268:280	arg1	concentrations					313:326	concentrations	313:326	concentrations	313:326	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	1	54	with	microplastics	268:280	arg1	sizes					306:310	different particle sizes	287:310	different particle sizes	287:310	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	1	54	with	microplastics	268:280	arg1	materials					332:340	materials	332:340	materials	332:340	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	2	55	dep	Beij	569:572	arg1	vulgaris					578:585	C. vulgaris	575:585	C. vulgaris	575:585	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	56	theme	particle	477:484	arg1	PS-MPs					522:527	PS-MPs	522:527	PS-MPs	522:527	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	56	theme	particle	477:484	arg1	sizes					486:490	particle sizes	477:490	particle sizes	477:490	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	3	57	theme	soluble	797:803	arg1	protein					805:811	the total soluble protein	787:811	the total soluble protein (TSP) contents	787:826	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	3	57	theme	soluble	797:803	arg1	TSP					814:816	TSP	814:816	TSP	814:816	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	5	58	theme	formation	1114:1122	arg1	contents					1151:1158	the colony formation and photosynthetic pigment contents	1103:1158	the colony formation and photosynthetic pigment contents	1103:1158	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	3	59	theme	increased	871:879	arg1	concentration					881:893	the increased concentration	867:893	the increased concentration of PE-MP exposure	867:911	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	3	60	theme	protein	805:811	arg1	contents					819:826	the total soluble protein (TSP) contents	787:826	the total soluble protein (TSP) contents	787:826	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	4	61	theme	photosynthetic	926:939	arg1	efficiency					941:950	photosynthetic efficiency	926:950	photosynthetic efficiency	926:950	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	3	62	theme	soluble	690:696	arg1	polysaccharides					712:726	soluble intracellular polysaccharides	690:726	soluble intracellular polysaccharides	690:726	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	5	63	theme	same	1256:1259	arg1	size					1270:1273	the same particle size	1252:1273	the same particle size	1252:1273	By contrast, the colony formation and photosynthetic pigment contents were remarkably decreased after exposure to PS-MPs compared with the control or PE-MPs with the same particle size.
37364300	1	64	from	ubiquitous	205:214	arg1	environments					235:246	various aquatic environments	219:246	various aquatic environments	219:246	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	1	65	theme	aquatic	227:233	arg1	environments					235:246	various aquatic environments	219:246	various aquatic environments	219:246	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	1	66	from	environments	235:246	arg1	ubiquitous					205:214	ubiquitous	205:214	ubiquitous	205:214	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	3	67	theme	pigment	669:675	arg1	contents					677:684	photosynthetic pigment contents	654:684	photosynthetic pigment contents	654:684	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	6	68	theme	main	1414:1417	arg1	pathways					1427:1434	the main harmful pathways	1410:1434	the main harmful pathways of different microplastics to algal	1410:1470	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	6	68	theme	main	1414:1417	arg1	effects					1397:1403	the adsorption effects	1382:1403	the adsorption effects	1382:1403	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	4	69	theme	efficiency	941:950	arg1	contents					999:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	6	70	theme	microplastics	1449:1461	arg1	pathways					1427:1434	the main harmful pathways	1410:1434	the main harmful pathways of different microplastics to algal	1410:1470	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	6	70	theme	microplastics	1449:1461	arg1	effects					1397:1403	the adsorption effects	1382:1403	the adsorption effects	1382:1403	The C. vulgaris colonization on microplastics was proven by scanning electron microscopy, indicating that the adsorption effects were the main harmful pathways of different microplastics to algal.
37364300	2	71	theme	microplastics	420:432	arg1	PS-MPs					522:527	PS-MPs	522:527	PS-MPs	522:527	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	71	theme	microplastics	420:432	arg1	sizes					486:490	particle sizes	477:490	particle sizes	477:490	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	71	theme	microplastics	420:432	arg1	microplastics					507:519	polystyrene microplastics	495:519	polystyrene microplastics	495:519	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	2	71	theme	microplastics	420:432	arg1	toxicity					395:402	the toxicity	391:402	the toxicity of polyethylene microplastics (PE-MPs) with different concentrations	391:471	This study investigated the toxicity of polyethylene microplastics (PE-MPs) with different concentrations and particle sizes or polystyrene microplastics (PS-MPs) on freshwater algae Chlorella vulgaris Beij (C. vulgaris) for 11 days.
37364300	4	72	theme	different	1054:1062	arg1	sizes					1073:1077	different particle sizes	1054:1077	different particle sizes	1054:1077	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
37364300	1	73	theme	plastic	151:157	arg1	particles					159:167	plastic particles	151:167	plastic particles	151:167	Microplastics, plastic particles and fragments smaller than 5 mm are ubiquitous in various aquatic environments, but the hazards of microplastics with different particle sizes, concentrations and materials are not well understood.
37364300	3	74	theme	exposure	904:911	arg1	concentration					881:893	the increased concentration	867:893	the increased concentration of PE-MP exposure	867:911	Results indicated that the growth, colony formation, photosynthetic pigment contents and soluble intracellular polysaccharides were unaffected, whereas the photosynthetic efficiency and the total soluble protein (TSP) contents were remarkably decreased at 11 d with the increased concentration of PE-MP exposure.
37364300	4	75	theme	polysaccharides	975:989	arg1	contents					999:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents	914:1006	The growth, photosynthetic efficiency, soluble intracellular polysaccharides and TSP contents were unaffected after exposure to PE-MPs with different particle sizes or PS-MPs.
35567041	10	0	theme	physicochemical	1533:1547	arg1	properties					1549:1558	good mechanical and physicochemical properties	1513:1558	good mechanical and physicochemical properties	1513:1558	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	1	1	theme	barrier	241:247	arg1	properties					249:258	low water barrier properties	231:258	low water barrier properties	231:258	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	6	2	theme	CMCy/RF	1041:1047	arg1	films					1059:1063	the CMCy/RF composite films	1037:1063	the CMCy/RF composite films	1037:1063	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	8	3	contain	had	1276:1278	arg1	samples					1268:1274	all film samples	1259:1274	all film samples	1259:1274	Based on SEM micrographs, all film samples had a relatively coarser surface.
35567041	8	3	contain	had	1276:1278	arg2	surface					1301:1307	a relatively coarser surface	1280:1307	a relatively coarser surface	1280:1307	Based on SEM micrographs, all film samples had a relatively coarser surface.
35567041	6	4	theme	p	1114:1114	arg1	<					1116:1116	p < 0.05	1114:1121	p < 0.05	1114:1121	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	1	5	theme	young	185:189	arg1	film					222:225	young Palmyra palm fruit husk (CMCy) film	185:225	young Palmyra palm fruit husk (CMCy) film	185:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	6	6	theme	decreased	1013:1021	arg1	yellowness					1023:1032	a coincidental decreased yellowness	998:1032	a coincidental decreased yellowness of the CMCy/RF composite films	998:1063	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	1	7	theme	palm	199:202	arg1	film					222:225	young Palmyra palm fruit husk (CMCy) film	185:225	young Palmyra palm fruit husk (CMCy) film	185:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	10	8	contain	has	1509:1511	arg2	properties					1549:1558	good mechanical and physicochemical properties	1513:1558	good mechanical and physicochemical properties	1513:1558	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	10	8	contain	has	1509:1511	arg1	it					1506:1507	it	1506:1507	it	1506:1507	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	0	9	theme	Fruit	129:133	arg1	Husk					135:138	Young Palmyra Palm Fruit Husk	110:138	Young Palmyra Palm Fruit Husk	110:138	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	1	10	theme	husk	210:213	arg1	film					222:225	young Palmyra palm fruit husk (CMCy) film	185:225	young Palmyra palm fruit husk (CMCy) film	185:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	1	11	contain	has	227:229	arg2	properties					249:258	low water barrier properties	231:258	low water barrier properties	231:258	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	1	11	contain	has	227:229	arg1	cellulose					170:178	Carboxymethyl cellulose	156:178	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film	156:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	1	12	theme	CMCy	216:219	arg1	film					222:225	young Palmyra palm fruit husk (CMCy) film	185:225	young Palmyra palm fruit husk (CMCy) film	185:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	10	13	theme	CMCy50/RF50	1434:1444	arg1	formulation					1486:1496	the best formulation	1477:1496	the best formulation	1477:1496	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	10	13	theme	CMCy50/RF50	1434:1444	arg1	film					1456:1459	the CMCy50/RF50 composite film	1430:1459	the CMCy50/RF50 composite film	1430:1459	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	8	14	theme	coarser	1293:1299	arg1	surface					1301:1307	a relatively coarser surface	1280:1307	a relatively coarser surface	1280:1307	Based on SEM micrographs, all film samples had a relatively coarser surface.
35567041	1	15	from	film	222:225	arg1	cellulose					170:178	Carboxymethyl cellulose	156:178	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film	156:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	3	16	dep	proportions	478:488	arg1	proportions					478:488	different proportions	468:488	different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100)	468:548	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	3	16	dep	proportions	478:488	arg1	CMCy25/RF75					526:536	CMCy25/RF75	526:536	CMCy25/RF75	526:536	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	3	16	dep	proportions	478:488	arg1	RF100					543:547	RF100	543:547	RF100	543:547	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	6	17	theme	films	1059:1063	arg1	yellowness					1023:1032	a coincidental decreased yellowness	998:1032	a coincidental decreased yellowness of the CMCy/RF composite films	998:1063	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	4	18	theme	film	619:622	arg1	strength					624:631	The film strength	615:631	The film strength (33.36−12.99 MPa)	615:649	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	4	18	theme	film	619:622	arg1	MPa					646:648	33.36−12.99 MPa	634:648	33.36−12.99 MPa	634:648	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	4	18	theme	film	619:622	arg1	%					677:677	9.81−3.95%	668:677	9.81−3.95%	668:677	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	0	19	theme	Carboxymethyl	81:93	arg1	Cellulose					95:103	Carboxymethyl Cellulose	81:103	Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour	81:153	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	5	20	theme	composite	940:948	arg1	films					950:954	the CMCy/RF composite films	928:954	the CMCy/RF composite films	928:954	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	21	theme	9.25−6.18	855:863	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	21	theme	9.25−6.18	855:863	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	4	22	theme	CMCy/RF	687:693	arg1	films					705:709	the CMCy/RF composite films	683:709	the CMCy/RF composite films	683:709	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	0	23	theme	Palmyra	116:122	arg1	Husk					135:138	Young Palmyra Palm Fruit Husk	110:138	Young Palmyra Palm Fruit Husk	110:138	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	5	24	theme	10−8	867:870	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	24	theme	10−8	867:870	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	9	25	dep	CMCy	1361:1364	arg1	films					1381:1385	blended films	1373:1385	blended films	1373:1385	FTIR spectra showed that some interactions between CMCy and RF blended films had occurred.
35567041	0	26	theme	Mechanical	0:9	arg1	Properties					31:40	Mechanical and Physicochemical Properties	0:40	Mechanical and Physicochemical Properties of Composite Biopolymer Films	0:70	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	5	27	theme	water	829:833	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	27	theme	water	829:833	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	27	theme	water	829:833	arg1	%					922:922	82.70−21.64%	911:922	82.70−21.64%	911:922	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	0	28	theme	Physicochemical	15:29	arg1	Properties					31:40	Mechanical and Physicochemical Properties	0:40	Mechanical and Physicochemical Properties of Composite Biopolymer Films	0:70	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	2	29	with	combination	304:314	arg1	flour					365:369	rice flour	360:369	rice flour (RF)	360:374	Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem.
35567041	2	29	with	combination	304:314	arg1	polysaccharides					335:349	other polysaccharides	329:349	other polysaccharides	329:349	Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem.
35567041	3	30	theme	mechanical	572:581	arg1	properties					603:612	their mechanical and physicochemical properties	566:612	their mechanical and physicochemical properties	566:612	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	4	31	theme	p	736:736	arg1	<					738:738	p < 0.05	736:743	p < 0.05	736:743	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	8	32	theme	SEM	1242:1244	arg1	micrographs					1246:1256	SEM micrographs	1242:1256	SEM micrographs	1242:1256	Based on SEM micrographs, all film samples had a relatively coarser surface.
35567041	2	33	theme	CMCy	319:322	arg1	combination					304:314	the combination	300:314	the combination of CMCy with other polysaccharides, such as rice flour (RF),	300:375	Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem.
35567041	0	34	theme	Biopolymer	55:64	arg1	Films					66:70	Composite Biopolymer Films	45:70	Composite Biopolymer Films	45:70	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	5	35	theme	film	894:897	arg1	solubility					899:908	film solubility	894:908	film solubility	894:908	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	7	36	from	addition	1138:1145	arg1	proportions					1166:1176	different proportions	1156:1176	different proportions	1156:1176	However, the addition of RF in different proportions did not influence the film thickness and transparency.
35567041	6	37	theme	increased	973:981	arg1	pronounced					1069:1078	pronounced	1069:1078	pronounced	1069:1078	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	6	37	theme	increased	973:981	arg1	lightness					983:991	an increased lightness	970:991	an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films	970:1063	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	4	38	theme	films	705:709	arg1	strength					624:631	The film strength	615:631	The film strength (33.36−12.99 MPa)	615:649	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	4	38	theme	films	705:709	arg1	MPa					646:648	33.36−12.99 MPa	634:648	33.36−12.99 MPa	634:648	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	4	38	theme	films	705:709	arg1	flexibility					655:665	flexibility	655:665	flexibility	655:665	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	4	38	theme	films	705:709	arg1	%					677:677	9.81−3.95%	668:677	9.81−3.95%	668:677	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	10	39	theme	good	1513:1516	arg1	properties					1549:1558	good mechanical and physicochemical properties	1513:1558	good mechanical and physicochemical properties	1513:1558	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	5	40	theme	m	874:874	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	40	theme	m	874:874	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	3	41	theme	physicochemical	587:601	arg1	properties					603:612	their mechanical and physicochemical properties	566:612	their mechanical and physicochemical properties	566:612	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	8	42	theme	film	1263:1266	arg1	samples					1268:1274	all film samples	1259:1274	all film samples	1259:1274	Based on SEM micrographs, all film samples had a relatively coarser surface.
35567041	3	43	theme	CMCy/RF	441:447	arg1	films					459:463	the CMCy/RF composite films	437:463	the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100)	437:548	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	4	44	dep	decreased	711:719	arg1	<					738:738	p < 0.05	736:743	p < 0.05	736:743	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	6	45	dep	increased	1103:1111	arg1	<					1116:1116	p < 0.05	1114:1121	p < 0.05	1114:1121	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	5	46	theme	s−1	880:882	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	46	theme	s−1	880:882	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	2	47	theme	other	329:333	arg1	flour					365:369	rice flour	360:369	rice flour (RF)	360:374	Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem.
35567041	2	47	theme	other	329:333	arg1	polysaccharides					335:349	other polysaccharides	329:349	other polysaccharides	329:349	Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem.
35567041	1	48	theme	water	235:239	arg1	properties					249:258	low water barrier properties	231:258	low water barrier properties	231:258	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	6	49	with	lightness	983:991	arg1	yellowness					1023:1032	a coincidental decreased yellowness	998:1032	a coincidental decreased yellowness of the CMCy/RF composite films	998:1063	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	3	50	theme	different	468:476	arg1	CMCy25/RF75					526:536	CMCy25/RF75	526:536	CMCy25/RF75	526:536	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	3	50	theme	different	468:476	arg1	proportions					478:488	different proportions	468:488	different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100)	468:548	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	3	50	theme	different	468:476	arg1	RF100					543:547	RF100	543:547	RF100	543:547	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	4	51	theme	RF	770:771	arg1	proportion					773:782	the RF proportion	766:782	the RF proportion	766:782	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	0	52	from	Flour	149:153	arg1	Cellulose					95:103	Carboxymethyl Cellulose	81:103	Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour	81:153	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	7	53	dep	film	1200:1203	arg1	thickness					1205:1213	thickness	1205:1213	thickness	1205:1213	However, the addition of RF in different proportions did not influence the film thickness and transparency.
35567041	1	54	theme	Palmyra	191:197	arg1	film					222:225	young Palmyra palm fruit husk (CMCy) film	185:225	young Palmyra palm fruit husk (CMCy) film	185:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	6	55	theme	coincidental	1000:1011	arg1	yellowness					1023:1032	a coincidental decreased yellowness	998:1032	a coincidental decreased yellowness of the CMCy/RF composite films	998:1063	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	1	56	theme	fruit	204:208	arg1	film					222:225	young Palmyra palm fruit husk (CMCy) film	185:225	young Palmyra palm fruit husk (CMCy) film	185:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	0	57	theme	Rice	144:147	arg1	Flour					149:153	Rice Flour	144:153	Rice Flour	144:153	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	6	58	theme	RF	1089:1090	arg1	proportion					1092:1101	the RF proportion	1085:1101	the RF proportion	1085:1101	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	10	59	theme	composite	1446:1454	arg1	formulation					1486:1496	the best formulation	1477:1496	the best formulation	1477:1496	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	10	59	theme	composite	1446:1454	arg1	film					1456:1459	the CMCy50/RF50 composite film	1430:1459	the CMCy50/RF50 composite film	1430:1459	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	5	60	theme	CMCy/RF	932:938	arg1	films					950:954	the CMCy/RF composite films	928:954	the CMCy/RF composite films	928:954	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	6	61	theme	composite	1049:1057	arg1	films					1059:1063	the CMCy/RF composite films	1037:1063	the CMCy/RF composite films	1037:1063	Furthermore, an increased lightness with a coincidental decreased yellowness of the CMCy/RF composite films was pronounced when the RF proportion increased (p < 0.05).
35567041	3	62	from	films	459:463	arg1	CMCy25/RF75					526:536	CMCy25/RF75	526:536	CMCy25/RF75	526:536	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	3	62	from	films	459:463	arg1	proportions					478:488	different proportions	468:488	different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100)	468:548	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	3	62	from	films	459:463	arg1	RF100					543:547	RF100	543:547	RF100	543:547	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	1	63	theme	Carboxymethyl	156:168	arg1	cellulose					170:178	Carboxymethyl cellulose	156:178	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film	156:225	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35567041	4	64	from	increase	754:761	arg1	proportion					773:782	the RF proportion	766:782	the RF proportion	766:782	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	5	65	with	RF	798:799	arg1	CMCy					806:809	CMCy	806:809	CMCy	806:809	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	0	66	theme	Young	110:114	arg1	Husk					135:138	Young Palmyra Palm Fruit Husk	110:138	Young Palmyra Palm Fruit Husk	110:138	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	9	67	theme	blended	1373:1379	arg1	films					1381:1385	blended films	1373:1385	blended films	1373:1385	FTIR spectra showed that some interactions between CMCy and RF blended films had occurred.
35567041	0	68	theme	Palm	124:127	arg1	Husk					135:138	Young Palmyra Palm Fruit Husk	110:138	Young Palmyra Palm Fruit Husk	110:138	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	5	69	theme	films	950:954	arg1	solubility					899:908	film solubility	894:908	film solubility	894:908	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	69	theme	films	950:954	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	69	theme	films	950:954	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	69	theme	films	950:954	arg1	%					922:922	82.70−21.64%	911:922	82.70−21.64%	911:922	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	70	theme	×	865:865	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	70	theme	×	865:865	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	7	71	theme	different	1156:1164	arg1	proportions					1166:1176	different proportions	1156:1176	different proportions	1156:1176	However, the addition of RF in different proportions did not influence the film thickness and transparency.
35567041	2	72	theme	rice	360:363	arg1	RF					372:373	RF	372:373	RF	372:373	Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem.
35567041	2	72	theme	rice	360:363	arg1	flour					365:369	rice flour	360:369	rice flour (RF)	360:374	Thus, the combination of CMCy with other polysaccharides, such as rice flour (RF), may solve this problem.
35567041	7	73	theme	RF	1150:1151	arg1	addition					1138:1145	the addition	1134:1145	the addition of RF in different proportions	1134:1176	However, the addition of RF in different proportions did not influence the film thickness and transparency.
35567041	5	74	theme	vapor	835:839	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	74	theme	vapor	835:839	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	74	theme	vapor	835:839	arg1	%					922:922	82.70−21.64%	911:922	82.70−21.64%	911:922	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	0	75	theme	Composite	45:53	arg1	Films					66:70	Composite Biopolymer Films	45:70	Composite Biopolymer Films	45:70	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	10	76	theme	best	1481:1484	arg1	formulation					1486:1496	the best formulation	1477:1496	the best formulation	1477:1496	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	10	76	theme	best	1481:1484	arg1	film					1456:1459	the CMCy50/RF50 composite film	1430:1459	the CMCy50/RF50 composite film	1430:1459	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	0	77	theme	Films	66:70	arg1	Properties					31:40	Mechanical and Physicochemical Properties	0:40	Mechanical and Physicochemical Properties of Composite Biopolymer Films	0:70	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	10	78	theme	mechanical	1518:1527	arg1	properties					1549:1558	good mechanical and physicochemical properties	1513:1558	good mechanical and physicochemical properties	1513:1558	According to these findings, the CMCy50/RF50 composite film was found to be the best formulation because it has good mechanical and physicochemical properties.
35567041	5	79	theme	g	872:872	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	79	theme	g	872:872	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	4	80	theme	composite	695:703	arg1	films					705:709	the CMCy/RF composite films	683:709	the CMCy/RF composite films	683:709	The film strength (33.36−12.99 MPa) and flexibility (9.81−3.95%) of the CMCy/RF composite films decreased significantly (p < 0.05) with an increase in the RF proportion.
35567041	3	81	theme	study	417:421	arg1	aim					405:407	The aim	401:407	The aim of this study	401:421	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	9	82	theme	FTIR	1310:1313	arg1	spectra					1315:1321	FTIR spectra	1310:1321	FTIR spectra	1310:1321	FTIR spectra showed that some interactions between CMCy and RF blended films had occurred.
35567041	5	83	theme	m−2	876:878	arg1	permeability					841:852	the water vapor permeability	825:852	the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1)	825:888	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	5	83	theme	m−2	876:878	arg1	Pa−1					884:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	9.25−6.18 × 10−8 g m m−2 s−1 Pa−1	855:887	Blending the RF with CMCy could improve the water vapor permeability (9.25−6.18 × 10−8 g m m−2 s−1 Pa−1) and film solubility (82.70−21.64%) of the CMCy/RF composite films.
35567041	0	84	from	Husk	135:138	arg1	Cellulose					95:103	Carboxymethyl Cellulose	81:103	Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour	81:153	Mechanical and Physicochemical Properties of Composite Biopolymer Films Based on Carboxymethyl Cellulose from Young Palmyra Palm Fruit Husk and Rice Flour.
35567041	3	85	theme	composite	449:457	arg1	films					459:463	the CMCy/RF composite films	437:463	the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100)	437:548	The aim of this study is to prepare the CMCy/RF composite films in different proportions (CMCy100, CMCy75/RF25, CMCy50/RF50, CMCy25/RF75, and RF100) and investigate their mechanical and physicochemical properties.
35567041	1	86	theme	low	231:233	arg1	properties					249:258	low water barrier properties	231:258	low water barrier properties	231:258	Carboxymethyl cellulose from young Palmyra palm fruit husk (CMCy) film has low water barrier properties, which can limit its application.
35182063	14	0	theme	wet	2430:2432	arg1	conditions					2442:2451	wet and dry conditions	2430:2451	conditions	2442:2451	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	6	1	theme	composite	1233:1241	arg1	scaffolds					1243:1251	the prepared composite scaffolds	1220:1251	the prepared composite scaffolds	1220:1251	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	9	2	theme	scaffolds	1586:1594	arg1	properties					1558:1567	The mechanical properties	1543:1567	The mechanical properties of the composite scaffolds	1543:1594	The mechanical properties of the composite scaffolds were investigated under different conditions.
35182063	6	3	theme	mechanical	1172:1181	arg1	property					1183:1190	mechanical property	1172:1190	mechanical property	1172:1190	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	0	4	dep	In	16:17	arg1	Vitro					19:23	Vitro	19:23	Vitro	19:23	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	14	5	theme	PBS	2294:2296	arg1	absorption					2298:2307	PBS absorption	2294:2307	PBS absorption	2294:2307	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	10	6	theme	composite	1683:1691	arg1	specimens					1693:1701	the composite specimens	1679:1701	the composite specimens	1679:1701	The results demonstrated that all of the composite specimens had better strength when compared with the GEL/CS nanofibres.
35182063	5	7	theme	electrospun	853:863	arg1	PCL					865:867	electrospun PCL	853:867	we electrospun PCL	850:867	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	14	8	theme	significant	2374:2384	arg1	improvements					2386:2397	significant improvements	2374:2397	significant improvements in mechanical properties under wet and dry conditions	2374:2451	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	9	9	theme	different	1620:1628	arg1	conditions					1630:1639	different conditions	1620:1639	different conditions	1620:1639	The mechanical properties of the composite scaffolds were investigated under different conditions.
35182063	6	10	theme	phosphate-buffered	1108:1125	arg1	PBS					1135:1137	PBS	1135:1137	PBS	1135:1137	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	6	10	theme	phosphate-buffered	1108:1125	arg1	saline					1127:1132	phosphate-buffered saline	1108:1132	phosphate-buffered saline (PBS) absorption	1108:1149	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	5	11	dep	2:1	1009:1011	arg1	1:2					1022:1024	1:2	1022:1024	1:2	1022:1024	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	5	11	dep	2:1	1009:1011	arg1	1:1					1014:1016	1:1	1014:1016	1:1	1014:1016	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	6	12	theme	in	1196:1197	arg1	bioactivity					1205:1215	in vitro bioactivity	1196:1215	in vitro bioactivity	1196:1215	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	2	13	theme	alongside	320:328	arg1	proteins					330:337	PCL alongside proteins	316:337	PCL alongside proteins	316:337	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	11	14	from	increase	1769:1776	arg1	ratio					1785:1789	PCL ratio	1781:1789	PCL ratio	1781:1789	The increase in PCL ratio led to an increase in tensile strength of the nanofibres.
35182063	12	15	theme	composite	1912:1920	arg1	scaffolds					1922:1930	the fabricated composite scaffolds	1897:1930	the fabricated composite scaffolds	1897:1930	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	5	16	dep	ratios	1001:1006	arg1	2:1					1009:1011	2:1	1009:1011	2:1	1009:1011	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	0	17	theme	Dermal	131:136	arg1	Engineering					145:155	Dermal Tissue Engineering	131:155	Dermal Tissue Engineering	131:155	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	8	18	dep	increase	1515:1522	arg1	addition					1489:1496	addition	1489:1496	addition	1489:1496	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	1	19	theme	considerable	199:210	arg1	properties					238:247	considerable mechanical and biological properties	199:247	considerable mechanical and biological properties that make it a good candidate for tissue engineering applications	199:313	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	13	20	theme	tissue	2176:2181	arg1	applications					2195:2206	skin tissue engineering applications	2171:2206	skin tissue engineering applications	2171:2206	The results showed the bioactivity of these nanofibres and the potential for these scaffolds to be used for skin tissue engineering applications.
35182063	3	21	theme	nanofibrous	600:610	arg1	scaffold					612:619	novel composite nanofibrous scaffold	584:619	novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process	584:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	7	22	theme	beadless	1325:1332	arg1	nanofibres					1334:1343	beadless nanofibres	1325:1343	beadless nanofibres	1325:1343	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	4	23	theme	experimental	729:740	arg1	study					742:746	this experimental study	724:746	this experimental study	724:746	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	3	24	theme	novel	584:588	arg1	scaffold					612:619	novel composite nanofibrous scaffold	584:619	novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process	584:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	5	25	theme	same	907:910	arg1	drum					912:915	the same drum	903:915	the same drum	903:915	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	0	26	from	Use	124:126	arg1	Engineering					145:155	Dermal Tissue Engineering	131:155	Dermal Tissue Engineering	131:155	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	13	27	used	used	2162:2165	arg2	scaffolds					2146:2154	these scaffolds	2140:2154	these scaffolds	2140:2154	The results showed the bioactivity of these nanofibres and the potential for these scaffolds to be used for skin tissue engineering applications.
35182063	12	28	theme	-5-	1978:1980	arg1	analysis					2045:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	14	29	theme	co-electrospun	2235:2248	arg1	scaffolds					2260:2268	The fabricated co-electrospun composite scaffolds	2220:2268	The fabricated co-electrospun composite scaffolds	2220:2268	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	0	30	theme	Sulphate-Polycaprolactone	53:77	arg1	Scaffold					101:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	10	31	contain	had	1703:1705	arg1	all					1672:1674	all	1672:1674	all	1672:1674	The results demonstrated that all of the composite specimens had better strength when compared with the GEL/CS nanofibres.
35182063	10	31	contain	had	1703:1705	arg2	strength					1714:1721	better strength	1707:1721	better strength	1707:1721	The results demonstrated that all of the composite specimens had better strength when compared with the GEL/CS nanofibres.
35182063	10	31	contain	had	1703:1705	arg1	specimens					1693:1701	the composite specimens	1679:1701	the composite specimens	1679:1701	The results demonstrated that all of the composite specimens had better strength when compared with the GEL/CS nanofibres.
35182063	0	32	theme	Nanofibrous	89:99	arg1	Scaffold					101:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	12	33	theme	Human	1849:1853	arg1	HDF					1875:1877	HDF	1875:1877	HDF	1875:1877	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	12	33	theme	Human	1849:1853	arg1	fibroblasts					1862:1872	Human dermal fibroblasts	1849:1872	Human dermal fibroblasts (HDF)	1849:1878	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	2	34	theme	engineering	523:533	arg1	scaffolds					535:543	skin tissue engineering scaffolds	511:543	skin tissue engineering scaffolds	511:543	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	5	35	theme	composite	954:962	arg1	scaffolds					976:984	composite nanofibrous scaffolds	954:984	composite nanofibrous scaffolds	954:984	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	2	36	theme	skin	511:514	arg1	scaffolds					535:543	skin tissue engineering scaffolds	511:543	skin tissue engineering scaffolds	511:543	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	14	37	from	improvements	2386:2397	arg1	properties					2413:2422	mechanical properties	2402:2422	mechanical properties under wet and dry conditions	2402:2451	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	11	38	theme	nanofibres	1837:1846	arg1	strength					1821:1828	tensile strength	1813:1828	tensile strength of the nanofibres	1813:1846	The increase in PCL ratio led to an increase in tensile strength of the nanofibres.
35182063	8	39	theme	PCL	1459:1461	arg1	scaffolds					1475:1483	the PCL nanofibrous scaffolds	1455:1483	the PCL nanofibrous scaffolds	1455:1483	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	2	40	theme	chondroitin	383:393	arg1	sulphate					395:402	chondroitin sulphate	383:402	chondroitin sulphate (CS)	383:407	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	2	40	theme	chondroitin	383:393	arg1	CS					405:406	CS	405:406	CS	405:406	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	5	41	theme	different	926:934	arg1	nozzles					936:942	different nozzles	926:942	different nozzles	926:942	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	7	42	theme	electron	1285:1292	arg1	SEM					1306:1308	SEM	1306:1308	SEM	1306:1308	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	7	42	theme	electron	1285:1292	arg1	microscopy					1294:1303	Scanning electron microscopy	1276:1303	Scanning electron microscopy (SEM) images	1276:1316	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	0	43	theme	In	16:17	arg1	Evaluation					25:34	In Vitro Evaluation	16:34	In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering	16:155	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	1	44	theme	engineering	290:300	arg1	applications					302:313	tissue engineering applications	283:313	tissue engineering applications	283:313	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	3	45	theme	PCL	661:663	arg1	ratios					640:645	various ratios	632:645	various ratios of GEL/CS and PCL using co-electrospinning process	632:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	14	46	theme	GEL/CS	2469:2474	arg1	scaffold					2476:2483	the GEL/CS scaffold	2465:2483	the GEL/CS scaffold	2465:2483	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	3	47	theme	GEL/CS	650:655	arg1	ratios					640:645	various ratios	632:645	various ratios of GEL/CS and PCL using co-electrospinning process	632:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	5	48	dep	we	850:851	arg1	PCL					865:867	electrospun PCL	853:867	we electrospun PCL	850:867	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	14	49	dep	CONCLUSION	2209:2218	arg1	had					2270:2272	had	2270:2272	had	2270:2272	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	8	50	theme	significant	1503:1513	arg1	increase					1515:1522	a significant increase	1501:1522	a significant increase in PBS absorption	1501:1540	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	1	51	theme	biological	227:236	arg1	properties					238:247	considerable mechanical and biological properties	199:247	considerable mechanical and biological properties that make it a good candidate for tissue engineering applications	199:313	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	5	52	theme	different	991:999	arg1	ratios					1001:1006	different ratios	991:1006	different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL	991:1041	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	3	53	theme	co-electrospinning	671:688	arg1	process					690:696	co-electrospinning process	671:696	co-electrospinning process	671:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	8	54	theme	composite	1381:1389	arg1	scaffolds					1391:1399	The composite scaffolds	1377:1399	The composite scaffolds (2:1, 1:1 and 1:2)	1377:1418	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	14	55	theme	nanofibrous	2336:2346	arg1	scaffolds					2348:2356	the PCL nanofibrous scaffolds	2328:2356	the PCL nanofibrous scaffolds	2328:2356	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	11	56	theme	tensile	1813:1819	arg1	strength					1821:1828	tensile strength	1813:1828	tensile strength of the nanofibres	1813:1846	The increase in PCL ratio led to an increase in tensile strength of the nanofibres.
35182063	6	57	dep	in	1196:1197	arg1	vitro					1199:1203	vitro	1199:1203	vitro	1199:1203	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	7	58	dep	RESULTS	1268:1274	arg1	showed					1318:1323	showed	1318:1323	showed beadless nanofibres at all ratios of GEL to CS-PCL	1318:1374	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	6	59	theme	scaffolds	1243:1251	arg1	structure					1161:1169	chemical structure	1152:1169	chemical structure	1152:1169	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	6	59	theme	scaffolds	1243:1251	arg1	Morphology					1086:1095	Morphology	1086:1095	Morphology	1086:1095	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	6	59	theme	scaffolds	1243:1251	arg1	bioactivity					1205:1215	in vitro bioactivity	1196:1215	in vitro bioactivity	1196:1215	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	6	59	theme	scaffolds	1243:1251	arg1	property					1183:1190	mechanical property	1172:1190	mechanical property	1172:1190	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	6	59	theme	scaffolds	1243:1251	arg1	porosity					1098:1105	porosity	1098:1105	porosity	1098:1105	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	6	59	theme	scaffolds	1243:1251	arg1	absorption					1140:1149	phosphate-buffered saline (PBS) absorption	1108:1149	phosphate-buffered saline (PBS) absorption	1108:1149	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	4	60	from	METHODS	713:719	arg1	study					742:746	this experimental study	724:746	this experimental study	724:746	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	12	61	theme	4,5-dimethylthiazol-	1952:1971	arg1	3-					1949:1950	3-	1949:1950	3-	1949:1950	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	12	61	theme	4,5-dimethylthiazol-	1952:1971	arg1	2-yl					1973:1976	4,5-dimethylthiazol- 2-yl	1952:1976	4,5-dimethylthiazol- 2-yl	1952:1976	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	9	62	theme	composite	1576:1584	arg1	scaffolds					1586:1594	the composite scaffolds	1572:1594	the composite scaffolds	1572:1594	The mechanical properties of the composite scaffolds were investigated under different conditions.
35182063	6	63	theme	prepared	1224:1231	arg1	scaffolds					1243:1251	the prepared composite scaffolds	1220:1251	the prepared composite scaffolds	1220:1251	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	4	64	theme	common	828:833	arg1	solvent					835:841	a common solvent	826:841	a common solvent	826:841	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	10	65	theme	better	1707:1712	arg1	strength					1714:1721	better strength	1707:1721	better strength	1707:1721	The results demonstrated that all of the composite specimens had better strength when compared with the GEL/CS nanofibres.
35182063	4	66	from	MATERIALS	699:707	arg1	study					742:746	this experimental study	724:746	this experimental study	724:746	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	1	67	theme	good	264:267	arg1	candidate					269:277	a good candidate	262:277	a good candidate for tissue engineering applications	262:313	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	7	68	theme	GEL	1362:1364	arg1	ratios					1352:1357	all ratios	1348:1357	all ratios of GEL to CS-PCL	1348:1374	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	14	69	theme	mechanical	2402:2411	arg1	properties					2413:2422	mechanical properties	2402:2422	mechanical properties under wet and dry conditions	2402:2451	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	2	70	used	used	417:420	arg2	proteins					330:337	PCL alongside proteins	316:337	PCL alongside proteins	316:337	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	2	70	used	used	417:420	arg2	polysaccharides					343:357	polysaccharides	343:357	polysaccharides	343:357	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	8	71	from	increase	1515:1522	arg1	absorption					1531:1540	PBS absorption	1527:1540	PBS absorption	1527:1540	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	4	72	theme	solvent	835:841	arg1	miscibility					798:808	miscibility	798:808	miscibility	798:808	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	4	72	theme	solvent	835:841	arg1	lack					818:821	the lack	814:821	the lack of a common solvent	814:841	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	6	73	theme	saline	1127:1132	arg1	absorption					1140:1149	phosphate-buffered saline (PBS) absorption	1108:1149	phosphate-buffered saline (PBS) absorption	1108:1149	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	12	74	theme	MTS	2040:2042	arg1	analysis					2045:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	3	75	theme	study	562:566	arg1	aim					550:552	The aim	546:552	The aim of this study	546:566	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	0	76	theme	Tissue	138:143	arg1	Engineering					145:155	Dermal Tissue Engineering	131:155	Dermal Tissue Engineering	131:155	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	14	77	theme	higher	2274:2279	arg1	porosity					2281:2288	higher porosity	2274:2288	higher porosity	2274:2288	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	2	78	theme	PCL	316:318	arg1	proteins					330:337	PCL alongside proteins	316:337	PCL alongside proteins	316:337	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	12	79	theme	fabricated	1901:1910	arg1	scaffolds					1922:1930	the fabricated composite scaffolds	1897:1930	the fabricated composite scaffolds	1897:1930	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	8	80	contain	had	1420:1422	arg1	scaffolds					1391:1399	The composite scaffolds	1377:1399	The composite scaffolds (2:1, 1:1 and 1:2)	1377:1418	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	8	80	contain	had	1420:1422	arg2	porosity					1434:1441	increased porosity	1424:1441	increased porosity	1424:1441	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	12	81	theme	4-sulfophenyl	2009:2021	arg1	analysis					2045:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	3	82	theme	composite	590:598	arg1	scaffold					612:619	novel composite nanofibrous scaffold	584:619	novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process	584:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	1	83	theme	mechanical	212:221	arg1	properties					238:247	considerable mechanical and biological properties	199:247	considerable mechanical and biological properties that make it a good candidate for tissue engineering applications	199:313	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	12	84	theme	-2H-tetrazolium	2023:2037	arg1	analysis					2045:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	11	85	theme	PCL	1781:1783	arg1	ratio					1785:1789	PCL ratio	1781:1789	PCL ratio	1781:1789	The increase in PCL ratio led to an increase in tensile strength of the nanofibres.
35182063	10	86	theme	GEL/CS	1746:1751	arg1	nanofibres					1753:1762	the GEL/CS nanofibres	1742:1762	the GEL/CS nanofibres	1742:1762	The results demonstrated that all of the composite specimens had better strength when compared with the GEL/CS nanofibres.
35182063	13	87	theme	skin	2171:2174	arg1	applications					2195:2206	skin tissue engineering applications	2171:2206	skin tissue engineering applications	2171:2206	The results showed the bioactivity of these nanofibres and the potential for these scaffolds to be used for skin tissue engineering applications.
35182063	3	88	theme	various	632:638	arg1	ratios					640:645	various ratios	632:645	various ratios of GEL/CS and PCL using co-electrospinning process	632:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	13	89	theme	engineering	2183:2193	arg1	applications					2195:2206	skin tissue engineering applications	2171:2206	skin tissue engineering applications	2171:2206	The results showed the bioactivity of these nanofibres and the potential for these scaffolds to be used for skin tissue engineering applications.
35182063	6	90	theme	chemical	1152:1159	arg1	structure					1161:1169	chemical structure	1152:1169	chemical structure	1152:1169	Morphology, porosity, phosphate-buffered saline (PBS) absorption, chemical structure, mechanical property and in vitro bioactivity of the prepared composite scaffolds were analysed.
35182063	12	91	theme	-2-	2005:2007	arg1	analysis					2045:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	14	92	theme	composite	2250:2258	arg1	scaffolds					2260:2268	The fabricated co-electrospun composite scaffolds	2220:2268	The fabricated co-electrospun composite scaffolds	2220:2268	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	0	93	theme	Composite	79:87	arg1	Scaffold					101:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	5	94	theme	GEL	1030:1032	arg1	ratios					1001:1006	different ratios	991:1006	different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL	991:1041	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	1	95	theme	OBJECTIVE	158:166	arg1	PCL					190:192	PCL	190:192	PCL	190:192	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	1	95	theme	OBJECTIVE	158:166	arg1	Poly					168:171	OBJECTIVE Poly	158:171	OBJECTIVE Poly(ε-caprolactone) (PCL)	158:193	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	1	95	theme	OBJECTIVE	158:166	arg1	ε-caprolactone					173:186	ε-caprolactone	173:186	ε-caprolactone	173:186	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	2	96	theme	biological	483:492	arg1	requirements					494:505	mechanical and biological requirements	468:505	mechanical and biological requirements for skin tissue engineering scaffolds	468:543	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	8	97	theme	increased	1424:1432	arg1	porosity					1434:1441	increased porosity	1424:1441	increased porosity	1424:1441	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	3	98	contain	containing	621:630	arg2	ratios					640:645	various ratios	632:645	various ratios of GEL/CS and PCL using co-electrospinning process	632:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	3	98	contain	containing	621:630	arg1	scaffold					612:619	novel composite nanofibrous scaffold	584:619	novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process	584:696	The aim of this study was fabricating novel composite nanofibrous scaffold containing various ratios of GEL/CS and PCL using co-electrospinning process.
35182063	14	99	theme	fabricated	2224:2233	arg1	scaffolds					2260:2268	The fabricated co-electrospun composite scaffolds	2220:2268	The fabricated co-electrospun composite scaffolds	2220:2268	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	0	100	theme	Scaffold	101:108	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	0	100	theme	Scaffold	101:108	arg1	Evaluation					25:34	In Vitro Evaluation	16:34	In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering	16:155	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	12	101	theme	dermal	1855:1860	arg1	HDF					1875:1877	HDF	1875:1877	HDF	1875:1877	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	12	101	theme	dermal	1855:1860	arg1	fibroblasts					1862:1872	Human dermal fibroblasts	1849:1872	Human dermal fibroblasts (HDF)	1849:1878	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	4	102	dep	blend	773:777	arg1	has					779:781	has	779:781	has limitations in miscibility and the lack of a common solvent	779:841	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	2	103	theme	mechanical	468:477	arg1	requirements					494:505	mechanical and biological requirements	468:505	mechanical and biological requirements for skin tissue engineering scaffolds	468:543	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	11	104	from	increase	1801:1808	arg1	strength					1821:1828	tensile strength	1813:1828	tensile strength of the nanofibres	1813:1846	The increase in PCL ratio led to an increase in tensile strength of the nanofibres.
35182063	0	105	theme	Potential	114:122	arg1	Use					124:126	Potential Use	114:126	Potential Use in Dermal Tissue Engineering	114:155	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	9	106	theme	mechanical	1547:1556	arg1	properties					1558:1567	The mechanical properties	1543:1567	The mechanical properties of the composite scaffolds	1543:1594	The mechanical properties of the composite scaffolds were investigated under different conditions.
35182063	2	107	theme	tissue	516:521	arg1	scaffolds					535:543	skin tissue engineering scaffolds	511:543	skin tissue engineering scaffolds	511:543	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	4	108	theme	GEL/CS	766:771	arg1	blend					773:777	a GEL/CS blend	764:777	a GEL/CS blend has limitations in miscibility and the lack of a common solvent	764:841	MATERIALS AND METHODS In this experimental study, PCL mixed with a GEL/CS blend has limitations in miscibility and the lack of a common solvent.
35182063	5	109	theme	nanofibrous	964:974	arg1	scaffolds					976:984	composite nanofibrous scaffolds	954:984	composite nanofibrous scaffolds	954:984	Here, we electrospun PCL and GEL/CS coincide separately on the same drum by using different nozzles to create composite nanofibrous scaffolds with different ratios (2:1, 1:1 and 1:2) of GEL to CSPCL, and we mixed them at the micro/nanoscale.
35182063	7	110	theme	microscopy	1294:1303	arg1	images					1311:1316	Scanning electron microscopy (SEM) images	1276:1316	Scanning electron microscopy (SEM) images	1276:1316	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	8	111	theme	nanofibrous	1463:1473	arg1	scaffolds					1475:1483	the PCL nanofibrous scaffolds	1455:1483	the PCL nanofibrous scaffolds	1455:1483	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	7	112	theme	Scanning	1276:1283	arg1	SEM					1306:1308	SEM	1306:1308	SEM	1306:1308	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	7	112	theme	Scanning	1276:1283	arg1	microscopy					1294:1303	Scanning electron microscopy	1276:1303	Scanning electron microscopy (SEM) images	1276:1316	RESULTS Scanning electron microscopy (SEM) images showed beadless nanofibres at all ratios of GEL to CS-PCL.
35182063	1	113	contain	has	195:197	arg1	PCL					190:192	PCL	190:192	PCL	190:192	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	1	113	contain	has	195:197	arg1	Poly					168:171	OBJECTIVE Poly	158:171	OBJECTIVE Poly(ε-caprolactone) (PCL)	158:193	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	1	113	contain	has	195:197	arg2	properties					238:247	considerable mechanical and biological properties	199:247	considerable mechanical and biological properties that make it a good candidate for tissue engineering applications	199:313	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	1	113	contain	has	195:197	arg1	ε-caprolactone					173:186	ε-caprolactone	173:186	ε-caprolactone	173:186	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	1	114	theme	tissue	283:288	arg1	applications					302:313	tissue engineering applications	283:313	tissue engineering applications	283:313	OBJECTIVE Poly(ε-caprolactone) (PCL) has considerable mechanical and biological properties that make it a good candidate for tissue engineering applications.
35182063	14	115	contain	had	2270:2272	arg1	scaffolds					2260:2268	The fabricated co-electrospun composite scaffolds	2220:2268	The fabricated co-electrospun composite scaffolds	2220:2268	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	14	115	contain	had	2270:2272	arg2	absorption					2298:2307	PBS absorption	2294:2307	PBS absorption	2294:2307	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	14	115	contain	had	2270:2272	arg2	porosity					2281:2288	higher porosity	2274:2288	higher porosity	2274:2288	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	14	116	with	comparison	2312:2321	arg1	scaffolds					2348:2356	the PCL nanofibrous scaffolds	2328:2356	the PCL nanofibrous scaffolds	2328:2356	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	0	117	theme	Chondroitin	41:51	arg1	Scaffold					101:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold	39:108	Fabrication and In Vitro Evaluation of A Chondroitin Sulphate-Polycaprolactone Composite Nanofibrous Scaffold for Potential Use in Dermal Tissue Engineering.
35182063	13	118	theme	nanofibres	2107:2116	arg1	bioactivity					2086:2096	the bioactivity	2082:2096	the bioactivity of these nanofibres	2082:2116	The results showed the bioactivity of these nanofibres and the potential for these scaffolds to be used for skin tissue engineering applications.
35182063	13	118	theme	nanofibres	2107:2116	arg1	potential					2126:2134	the potential	2122:2134	the potential for these scaffolds to be used for skin tissue engineering applications	2122:2206	The results showed the bioactivity of these nanofibres and the potential for these scaffolds to be used for skin tissue engineering applications.
35182063	12	119	theme	3-	1949:1950	arg1	-5-					1978:1980	3-(4,5-dimethylthiazol- 2-yl)-5-	1949:1980	3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis	1949:2052	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	12	119	theme	3-	1949:1950	arg1	carboxymethoxyphenyl					1984:2003	3 carboxymethoxyphenyl	1982:2003	3 carboxymethoxyphenyl	1982:2003	Human dermal fibroblasts (HDF) were cultured on the fabricated composite scaffolds and evaluated by 3-(4,5-dimethylthiazol- 2-yl)-5-(3 carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) analysis and SEM.
35182063	2	120	theme	composite	435:443	arg1	scaffolds					445:453	composite scaffolds	435:453	composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds	435:543	PCL alongside proteins and polysaccharides, like gelatin (GEL) and chondroitin sulphate (CS), can be used to fabricate composite scaffolds that provide mechanical and biological requirements for skin tissue engineering scaffolds.
35182063	8	121	dep	scaffolds	1391:1399	arg1	2:1					1402:1404	2:1	1402:1404	2:1	1402:1404	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	8	121	dep	scaffolds	1391:1399	arg1	1:2					1415:1417	1:2	1415:1417	1:2	1415:1417	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	8	121	dep	scaffolds	1391:1399	arg1	1:1					1407:1409	1:1	1407:1409	1:1	1407:1409	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	8	122	theme	PBS	1527:1529	arg1	absorption					1531:1540	PBS absorption	1527:1540	PBS absorption	1527:1540	The composite scaffolds (2:1, 1:1 and 1:2) had increased porosity compared to the PCL nanofibrous scaffolds, in addition to a significant increase in PBS absorption.
35182063	14	123	theme	dry	2438:2440	arg1	conditions					2442:2451	wet and dry conditions	2430:2451	conditions	2442:2451	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35182063	14	124	theme	PCL	2332:2334	arg1	scaffolds					2348:2356	the PCL nanofibrous scaffolds	2328:2356	the PCL nanofibrous scaffolds	2328:2356	CONCLUSION The fabricated co-electrospun composite scaffolds had higher porosity and PBS absorption in comparison with the PCL nanofibrous scaffolds, in addition to significant improvements in mechanical properties under wet and dry conditions compared to the GEL/CS scaffold.
35947430	5	0	theme	high-molecular-weight	762:782	arg1	fraction					799:806	the high-molecular-weight polysaccharide fraction	758:806	the high-molecular-weight polysaccharide fraction	758:806	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	7	1	theme	same	1207:1210	arg1	system					1225:1230	the same fermentation system	1203:1230	the same fermentation system	1203:1230	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	1	2	theme	pivotal	136:142	arg1	problems					144:151	the pivotal problems	132:151	the pivotal problems affecting beer flavor and production	132:188	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	5	3	theme	polysaccharide	784:797	arg1	fraction					799:806	the high-molecular-weight polysaccharide fraction	758:806	the high-molecular-weight polysaccharide fraction	758:806	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	1	4	theme	problems	144:151	arg1	one					125:127	one	125:127	one	125:127	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	1	4	theme	problems	144:151	arg1	problems					144:151	the pivotal problems	132:151	the pivotal problems affecting beer flavor and production	132:188	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	6	5	theme	zeta	988:991	arg1	potential					993:1001	a more negative zeta potential	972:1001	a more negative zeta potential than the control yeast	972:1024	In addition, the PYF yeast showed a more negative zeta potential than the control yeast during the small-scale brewing process.
35947430	7	6	theme	charged	1100:1106	arg1	polysaccharides					1108:1122	the negatively charged polysaccharides	1085:1122	the negatively charged polysaccharides potentially bonded to the surface of yeast cells	1085:1171	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	2	7	theme	barley	282:287	arg1	husks					289:293	malted barley husks	275:293	malted barley husks	275:293	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	6	8	theme	negative	979:986	arg1	potential					993:1001	a more negative zeta potential	972:1001	a more negative zeta potential than the control yeast	972:1024	In addition, the PYF yeast showed a more negative zeta potential than the control yeast during the small-scale brewing process.
35947430	2	9	theme	malted	275:280	arg1	husks					289:293	malted barley husks	275:293	malted barley husks	275:293	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	2	10	dep	such	343:346	arg1	as					348:349	as	348:349	as	348:349	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	5	11	theme	zeta	869:872	arg1	potential					874:882	the most negative zeta potential	851:882	the most negative zeta potential	851:882	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	7	12	theme	calcium	1185:1191	arg1	cation					1193:1198	the calcium cation	1181:1198	the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation	1181:1285	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	4	13	theme	zeta	716:719	arg1	potential					721:729	zeta potential	716:729	zeta potential	716:729	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	7	14	theme	cells	1167:1171	arg1	surface					1150:1156	the surface	1146:1156	the surface of yeast cells	1146:1171	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	8	15	dep	Fusarium	1344:1351	arg1	graminearum					1353:1363	graminearum	1353:1363	graminearum	1353:1363	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	4	16	theme	molecular	655:663	arg1	distribution					670:681	molecular mass distribution	655:681	molecular mass distribution	655:681	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	5	17	theme	highest	816:822	arg1	content					824:830	the highest content	812:830	the highest content of uronic acid	812:845	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	2	18	dep	Fusarium	351:358	arg1	graminearum					360:370	graminearum	360:370	graminearum	360:370	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	6	19	theme	PYF	955:957	arg1	yeast					959:963	the PYF yeast	951:963	the PYF yeast	951:963	In addition, the PYF yeast showed a more negative zeta potential than the control yeast during the small-scale brewing process.
35947430	1	20	theme	beer	163:166	arg1	flavor					168:173	beer flavor	163:173	beer flavor	163:173	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	3	21	theme	barley	507:512	arg1	husk					514:517	the barley husk	503:517	the barley husk	503:517	In this research, the formation mechanism of PYF was uncovered by investigating the secretome of F. graminearum MH1 inoculated to the barley husk.
35947430	7	22	theme	fermentation	1212:1223	arg1	system					1225:1230	the same fermentation system	1203:1230	the same fermentation system	1203:1230	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	7	23	theme	rapid	1250:1254	arg1	flocculation					1256:1267	rapid flocculation	1250:1267	rapid flocculation	1250:1267	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	1	24	theme	Premature	87:95	arg1	PYF					117:119	PYF	117:119	PYF	117:119	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	1	24	theme	Premature	87:95	arg1	flocculation					103:114	Premature yeast flocculation	87:114	Premature yeast flocculation (PYF)	87:120	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	0	25	theme	Barley	0:5	arg1	Husk					7:10	Barley Husk	0:10	Barley Husk	0:10	Barley Husk Degraded by Fusarium graminearum MH1 Induced Premature Yeast Flocculation.
35947430	1	26	theme	yeast	97:101	arg1	PYF					117:119	PYF	117:119	PYF	117:119	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	1	26	theme	yeast	97:101	arg1	flocculation					103:114	Premature yeast flocculation	87:114	Premature yeast flocculation (PYF)	87:120	Premature yeast flocculation (PYF) is one of the pivotal problems affecting beer flavor and production.
35947430	3	27	theme	PYF	418:420	arg1	mechanism					405:413	the formation mechanism	391:413	the formation mechanism of PYF	391:420	In this research, the formation mechanism of PYF was uncovered by investigating the secretome of F. graminearum MH1 inoculated to the barley husk.
35947430	5	28	theme	uronic	835:840	arg1	acid					842:845	uronic acid	835:845	uronic acid	835:845	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	5	29	theme	severe	915:920	arg1	phenomenon					926:935	the most severe PYF phenomenon	906:935	the most severe PYF phenomenon	906:935	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	8	30	theme	proteins	1317:1324	arg1	%					1304:1304	Approximately 12%	1288:1304	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome	1288:1377	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	8	30	theme	proteins	1317:1324	arg1	proteins					1317:1324	the 214 proteins	1309:1324	the 214 proteins identified in the Fusarium graminearum MH1 secretome	1309:1377	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	4	31	theme	minimum	628:634	arg1	concentration					640:652	the minimum PYF concentration	624:652	the minimum PYF concentration	624:652	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	5	32	theme	PYF	922:924	arg1	phenomenon					926:935	the most severe PYF phenomenon	906:935	the most severe PYF phenomenon	906:935	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	4	33	theme	husk	559:562	arg1	extract					539:545	The polysaccharide extract	520:545	The polysaccharide extract of degraded husk	520:562	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	8	34	theme	PYF	1474:1476	arg1	yeast					1478:1482	PYF yeast	1474:1482	PYF yeast	1474:1482	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	2	35	theme	husks	289:293	arg1	degradation					260:270	the degradation	256:270	the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum	256:370	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	6	36	theme	small-scale	1037:1047	arg1	process					1057:1063	the small-scale brewing process	1033:1063	the small-scale brewing process	1033:1063	In addition, the PYF yeast showed a more negative zeta potential than the control yeast during the small-scale brewing process.
35947430	3	37	theme	formation	395:403	arg1	mechanism					405:413	the formation mechanism	391:413	the formation mechanism of PYF	391:420	In this research, the formation mechanism of PYF was uncovered by investigating the secretome of F. graminearum MH1 inoculated to the barley husk.
35947430	3	38	theme	MH1	485:487	arg1	secretome					457:465	the secretome	453:465	the secretome of F. graminearum MH1 inoculated to the barley husk	453:517	In this research, the formation mechanism of PYF was uncovered by investigating the secretome of F. graminearum MH1 inoculated to the barley husk.
35947430	7	39	from	cation	1193:1198	arg1	system					1225:1230	the same fermentation system	1203:1230	the same fermentation system	1203:1230	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	2	40	theme	non-starch	217:226	arg1	polysaccharides					228:242	certain non-starch polysaccharides	209:242	certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum	209:370	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	4	41	theme	mass	665:668	arg1	distribution					670:681	molecular mass distribution	655:681	molecular mass distribution	655:681	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	0	42	theme	Fusarium	24:31	arg1	Flocculation					73:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Barley Husk Degraded by Fusarium graminearum MH1 Induced Premature Yeast Flocculation.
35947430	2	43	theme	certain	209:215	arg1	polysaccharides					228:242	certain non-starch polysaccharides	209:242	certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum	209:370	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	4	44	theme	degraded	550:557	arg1	husk					559:562	degraded husk	550:562	degraded husk	550:562	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	4	45	theme	PYF	636:638	arg1	concentration					640:652	the minimum PYF concentration	624:652	the minimum PYF concentration	624:652	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	0	46	theme	Induced	49:55	arg1	Flocculation					73:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Barley Husk Degraded by Fusarium graminearum MH1 Induced Premature Yeast Flocculation.
35947430	8	47	theme	MH1	1365:1367	arg1	secretome					1369:1377	the Fusarium graminearum MH1 secretome	1340:1377	the Fusarium graminearum MH1 secretome	1340:1377	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	3	48	theme	graminearum	473:483	arg1	MH1					485:487	F. graminearum MH1	470:487	F. graminearum MH1 inoculated to the barley husk	470:517	In this research, the formation mechanism of PYF was uncovered by investigating the secretome of F. graminearum MH1 inoculated to the barley husk.
35947430	0	49	theme	MH1	45:47	arg1	Flocculation					73:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Barley Husk Degraded by Fusarium graminearum MH1 Induced Premature Yeast Flocculation.
35947430	4	50	theme	polysaccharide	524:537	arg1	extract					539:545	The polysaccharide extract	520:545	The polysaccharide extract of degraded husk	520:562	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	0	51	dep	Fusarium	24:31	arg1	graminearum					33:43	graminearum	33:43	graminearum	33:43	Barley Husk Degraded by Fusarium graminearum MH1 Induced Premature Yeast Flocculation.
35947430	2	52	theme	microorganisms	327:340	arg1	growth					304:309	the growth	300:309	the growth of contaminated microorganisms, such as Fusarium graminearum	300:370	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	7	53	theme	yeast	1161:1165	arg1	cells					1167:1171	yeast cells	1161:1171	yeast cells	1161:1171	This is aligned to the negatively charged polysaccharides potentially bonded to the surface of yeast cells through the calcium cation in the same fermentation system, which results in rapid flocculation and precipitation.
35947430	8	54	theme	beer	1491:1494	arg1	brewing					1496:1502	beer brewing	1491:1502	beer brewing	1491:1502	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	2	55	theme	contaminated	314:325	arg1	microorganisms					327:340	contaminated microorganisms	314:340	contaminated microorganisms	314:340	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
35947430	8	56	theme	Fusarium	1344:1351	arg1	secretome					1369:1377	the Fusarium graminearum MH1 secretome	1340:1377	the Fusarium graminearum MH1 secretome	1340:1377	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	5	57	contain	had	808:810	arg1	fraction					799:806	the high-molecular-weight polysaccharide fraction	758:806	the high-molecular-weight polysaccharide fraction	758:806	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	5	57	contain	had	808:810	arg2	potential					874:882	the most negative zeta potential	851:882	the most negative zeta potential	851:882	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	5	57	contain	had	808:810	arg2	content					824:830	the highest content	812:830	the highest content of uronic acid	812:845	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	3	58	theme	F.	470:471	arg1	MH1					485:487	F. graminearum MH1	470:487	F. graminearum MH1 inoculated to the barley husk	470:517	In this research, the formation mechanism of PYF was uncovered by investigating the secretome of F. graminearum MH1 inoculated to the barley husk.
35947430	6	59	theme	control	1012:1018	arg1	yeast					1020:1024	the control yeast	1008:1024	the control yeast	1008:1024	In addition, the PYF yeast showed a more negative zeta potential than the control yeast during the small-scale brewing process.
35947430	0	60	theme	Yeast	67:71	arg1	Flocculation					73:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Barley Husk Degraded by Fusarium graminearum MH1 Induced Premature Yeast Flocculation.
35947430	5	61	theme	negative	860:867	arg1	potential					874:882	the most negative zeta potential	851:882	the most negative zeta potential	851:882	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	8	62	theme	polysaccharides	1449:1463	arg1	mechanism					1436:1444	the mechanism	1432:1444	the mechanism of polysaccharides	1432:1463	Approximately 12% of the 214 proteins identified in the Fusarium graminearum MH1 secretome were hemicellulases, which substantially interpreted the mechanism of polysaccharides inducing PYF yeast during beer brewing.
35947430	6	63	theme	brewing	1049:1055	arg1	process					1057:1063	the small-scale brewing process	1033:1063	the small-scale brewing process	1033:1063	In addition, the PYF yeast showed a more negative zeta potential than the control yeast during the small-scale brewing process.
35947430	4	64	theme	monosaccharide	684:697	arg1	composition					699:709	monosaccharide composition	684:709	monosaccharide composition	684:709	The polysaccharide extract of degraded husk was ultrafiltrated into four fractions and characterized by the minimum PYF concentration, molecular mass distribution, monosaccharide composition, and zeta potential.
35947430	0	65	theme	Premature	57:65	arg1	Flocculation					73:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Fusarium graminearum MH1 Induced Premature Yeast Flocculation	24:84	Barley Husk Degraded by Fusarium graminearum MH1 Induced Premature Yeast Flocculation.
35947430	5	66	theme	acid	842:845	arg1	potential					874:882	the most negative zeta potential	851:882	the most negative zeta potential	851:882	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	5	66	theme	acid	842:845	arg1	content					824:830	the highest content	812:830	the highest content of uronic acid	812:845	Among the four fractions, the high-molecular-weight polysaccharide fraction had the highest content of uronic acid and the most negative zeta potential, which contributed to the most severe PYF phenomenon.
35947430	2	67	theme	Fusarium	351:358	arg1	microorganisms					327:340	contaminated microorganisms	314:340	contaminated microorganisms	314:340	PYF is induced by certain non-starch polysaccharides produced by the degradation of malted barley husks upon the growth of contaminated microorganisms, such as Fusarium graminearum.
36033281	11	0	with	relationship	1487:1498	arg1	susceptibility					1512:1525	caries susceptibility	1505:1525	caries susceptibility	1505:1525	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	11	1	theme	resistant	1376:1384	arg1	mice					1394:1397	caries resistant C3H/HeN mice	1369:1397	caries resistant C3H/HeN mice	1369:1397	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	6	2	theme	peptide	723:729	arg1	fingerprinting					736:749	peptide mass fingerprinting	723:749	peptide mass fingerprinting	723:749	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	4	3	theme	column	457:462	arg1	chromatography					464:477	HAP column chromatography	453:477	HAP column chromatography	453:477	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	11	4	theme	overall	1439:1445	arg1	adsorption					1462:1471	overall hydroxyapatite adsorption	1439:1471	overall hydroxyapatite adsorption	1439:1471	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	7	5	with	amplification	887:899	arg1	primer					930:935	a CAVI specific primer	914:935	a CAVI specific primer	914:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	4	6	from	quantity	498:505	arg1	C3H/HeN					543:549	C3H/HeN	543:549	C3H/HeN	543:549	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	9	7	theme	circular	1156:1163	arg1	spectra					1175:1181	distinct circular dichroism spectra	1147:1181	distinct circular dichroism spectra of the molecules	1147:1198	CAVI activity was estimated from distinct circular dichroism spectra of the molecules and found higher in C3H/HeN mice.
36033281	7	8	with	RNA	831:833	arg1	primer					930:935	a CAVI specific primer	914:935	a CAVI specific primer	914:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	11	9	theme	higher	1409:1414	arg1	activity					1421:1428	higher CAVI activity	1409:1428	higher CAVI activity	1409:1428	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	6	10	theme	specific	795:802	arg1	antibody					815:822	a specific polyclonal antibody	793:822	a specific polyclonal antibody	793:822	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	9	11	theme	dichroism	1165:1173	arg1	spectra					1175:1181	distinct circular dichroism spectra	1147:1181	distinct circular dichroism spectra of the molecules	1147:1198	CAVI activity was estimated from distinct circular dichroism spectra of the molecules and found higher in C3H/HeN mice.
36033281	2	12	theme	protein	277:283	arg1	composition					285:295	pellicle-forming saliva protein composition	253:295	pellicle-forming saliva protein composition	253:295	One potential reason seems to be a difference in pellicle-forming saliva protein composition.
36033281	7	13	theme	PCR	883:885	arg1	amplification					887:899	PCR amplification	883:899	PCR amplification of cDNA with a CAVI specific primer	883:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	11	14	theme	C3H/HeN	1386:1392	arg1	mice					1394:1397	caries resistant C3H/HeN mice	1369:1397	caries resistant C3H/HeN mice	1369:1397	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	1	15	contain	has	150:152	arg1	BALB/cA					142:148	BALB/cA	142:148	BALB/cA	142:148	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	1	15	contain	has	150:152	arg2	sensitivity					159:169	high sensitivity	154:169	high sensitivity	154:169	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	10	16	theme	amino	1305:1309	arg1	acid					1311:1314	one amino acid	1301:1314	one amino acid	1301:1314	In summary, the CAVI composition of BALB/cA and C3H/HeN differs in one amino acid and a glyco-chain modification.
36033281	7	17	theme	sequence	942:949	arg1	analysis					951:958	sequence analysis	942:958	sequence analysis	942:958	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	2	18	theme	saliva	270:275	arg1	composition					285:295	pellicle-forming saliva protein composition	253:295	pellicle-forming saliva protein composition	253:295	One potential reason seems to be a difference in pellicle-forming saliva protein composition.
36033281	7	19	theme	mouse	868:872	arg1	strains					874:880	both mouse strains	863:880	both mouse strains	863:880	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	4	20	from	proteins	531:538	arg1	C3H/HeN					543:549	C3H/HeN	543:549	C3H/HeN	543:549	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	4	21	theme	HAP	453:455	arg1	chromatography					464:477	HAP column chromatography	453:477	HAP column chromatography	453:477	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	6	22	dep	anhydrase	700:708	arg1	VI					710:711	VI	710:711	VI	710:711	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	2	23	theme	pellicle-forming	253:268	arg1	composition					285:295	pellicle-forming saliva protein composition	253:295	pellicle-forming saliva protein composition	253:295	One potential reason seems to be a difference in pellicle-forming saliva protein composition.
36033281	7	24	theme	strains	874:880	arg1	glands					853:858	the salivary glands	840:858	the salivary glands of both mouse strains	840:880	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	1	25	theme	high	154:157	arg1	sensitivity					159:169	high sensitivity	154:169	high sensitivity	154:169	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	8	26	dep	deviate	1061:1067	arg1	confirmed					1086:1094	confirmed	1086:1094	confirmed by mass analysis	1086:1111	Glyco-chains of CAVI deviate in one N-glycan, confirmed by mass analysis.
36033281	9	27	theme	molecules	1190:1198	arg1	spectra					1175:1181	distinct circular dichroism spectra	1147:1181	distinct circular dichroism spectra of the molecules	1147:1198	CAVI activity was estimated from distinct circular dichroism spectra of the molecules and found higher in C3H/HeN mice.
36033281	6	28	theme	polyclonal	804:813	arg1	antibody					815:822	a specific polyclonal antibody	793:822	a specific polyclonal antibody	793:822	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	1	29	theme	Caries	76:81	arg1	sensitivity					83:93	Caries sensitivity	76:93	Caries sensitivity	76:93	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	10	30	theme	CAVI	1250:1253	arg1	composition					1255:1265	the CAVI composition	1246:1265	the CAVI composition of BALB/cA and C3H/HeN	1246:1288	In summary, the CAVI composition of BALB/cA and C3H/HeN differs in one amino acid and a glyco-chain modification.
36033281	7	31	from	base	983:986	arg1	codon					991:995	codon 96	991:998	codon 96	991:998	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	8	32	theme	CAVI	1056:1059	arg1	Glyco-chains					1040:1051	Glyco-chains	1040:1051	Glyco-chains of CAVI	1040:1059	Glyco-chains of CAVI deviate in one N-glycan, confirmed by mass analysis.
36033281	5	33	theme	One-	572:575	arg1	electrophoresis					597:611	One- and two-dimensional electrophoresis	572:611	One- and two-dimensional electrophoresis	572:611	One- and two-dimensional electrophoresis showed 2 bands/spots with deviating migration.
36033281	9	34	theme	distinct	1147:1154	arg1	spectra					1175:1181	distinct circular dichroism spectra	1147:1181	distinct circular dichroism spectra of the molecules	1147:1198	CAVI activity was estimated from distinct circular dichroism spectra of the molecules and found higher in C3H/HeN mice.
36033281	3	35	theme	saliva	404:409	arg1	proteins					411:418	hydroxyapatite (HAP) adsorbed saliva proteins	374:418	hydroxyapatite (HAP) adsorbed saliva proteins	374:418	Here, we performed a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains.
36033281	0	36	theme	Proteome	0:7	arg1	analysis					9:16	Proteome analysis	0:16	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.	0:74	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.
36033281	7	37	theme	Total	825:829	arg1	RNA					831:833	Total RNA	825:833	Total RNA from the salivary glands of both mouse strains	825:880	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	3	38	dep	adsorbed	395:402	arg1	HAP					390:392	HAP	390:392	HAP	390:392	Here, we performed a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains.
36033281	3	38	dep	adsorbed	395:402	arg1	hydroxyapatite					374:387	hydroxyapatite	374:387	hydroxyapatite (HAP)	374:393	Here, we performed a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains.
36033281	7	39	theme	specific	921:928	arg1	primer					930:935	a CAVI specific primer	914:935	a CAVI specific primer	914:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	7	40	theme	salivary	844:851	arg1	glands					853:858	the salivary glands	840:858	the salivary glands of both mouse strains	840:880	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	3	41	theme	proteins	411:418	arg1	differences					359:369	differences	359:369	differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains	359:450	Here, we performed a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains.
36033281	0	42	theme	high	21:24	arg1	proteins					48:55	high affinity mouse saliva proteins	21:55	high affinity mouse saliva proteins	21:55	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.
36033281	5	43	theme	two-dimensional	581:595	arg1	electrophoresis					597:611	One- and two-dimensional electrophoresis	572:611	One- and two-dimensional electrophoresis	572:611	One- and two-dimensional electrophoresis showed 2 bands/spots with deviating migration.
36033281	10	44	theme	C3H/HeN	1282:1288	arg1	composition					1255:1265	the CAVI composition	1246:1265	the CAVI composition of BALB/cA and C3H/HeN	1246:1288	In summary, the CAVI composition of BALB/cA and C3H/HeN differs in one amino acid and a glyco-chain modification.
36033281	4	45	from	C3H/HeN	543:549	arg1	quantity					498:505	the quantity	494:505	the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA	494:569	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	11	46	theme	caries	1505:1510	arg1	susceptibility					1512:1525	caries susceptibility	1505:1525	caries susceptibility	1505:1525	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	7	47	theme	different	973:981	arg1	base					983:986	one different base	969:986	one different base in codon 96	969:998	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	10	48	theme	BALB/cA	1270:1276	arg1	composition					1255:1265	the CAVI composition	1246:1265	the CAVI composition of BALB/cA and C3H/HeN	1246:1288	In summary, the CAVI composition of BALB/cA and C3H/HeN differs in one amino acid and a glyco-chain modification.
36033281	2	49	from	difference	239:248	arg1	composition					285:295	pellicle-forming saliva protein composition	253:295	pellicle-forming saliva protein composition	253:295	One potential reason seems to be a difference in pellicle-forming saliva protein composition.
36033281	1	50	theme	low	187:189	arg1	sensitivity					191:201	low sensitivity	187:201	low sensitivity	187:201	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	0	51	theme	mouse	35:39	arg1	proteins					48:55	high affinity mouse saliva proteins	21:55	high affinity mouse saliva proteins	21:55	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.
36033281	11	52	theme	CAVI	1416:1419	arg1	activity					1421:1428	higher CAVI activity	1409:1428	higher CAVI activity	1409:1428	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	10	53	theme	glyco-chain	1322:1332	arg1	modification					1334:1345	a glyco-chain modification	1320:1345	a glyco-chain modification	1320:1345	In summary, the CAVI composition of BALB/cA and C3H/HeN differs in one amino acid and a glyco-chain modification.
36033281	6	54	theme	murine	684:689	arg1	anhydrase					700:708	murine carbonic anhydrase VI	684:711	murine carbonic anhydrase VI (CAVI)	684:718	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	6	54	theme	murine	684:689	arg1	CAVI					714:717	CAVI	714:717	CAVI	714:717	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	6	54	theme	murine	684:689	arg1	They					660:663	They	660:663	They	660:663	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	0	55	theme	affinity	26:33	arg1	proteins					48:55	high affinity mouse saliva proteins	21:55	high affinity mouse saliva proteins	21:55	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.
36033281	4	56	theme	saliva	524:529	arg1	proteins					531:538	high-affinity saliva proteins	510:538	high-affinity saliva proteins in C3H/HeN compared to BALB/cA	510:569	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	3	57	theme	adsorbed	395:402	arg1	proteins					411:418	hydroxyapatite (HAP) adsorbed saliva proteins	374:418	hydroxyapatite (HAP) adsorbed saliva proteins	374:418	Here, we performed a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains.
36033281	9	58	theme	C3H/HeN	1220:1226	arg1	mice					1228:1231	C3H/HeN mice	1220:1231	C3H/HeN mice	1220:1231	CAVI activity was estimated from distinct circular dichroism spectra of the molecules and found higher in C3H/HeN mice.
36033281	11	59	theme	caries	1369:1374	arg1	mice					1394:1397	caries resistant C3H/HeN mice	1369:1397	caries resistant C3H/HeN mice	1369:1397	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	3	60	theme	mouse	438:442	arg1	strains					444:450	these two mouse strains	428:450	these two mouse strains	428:450	Here, we performed a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains.
36033281	0	61	theme	proteins	48:55	arg1	analysis					9:16	Proteome analysis	0:16	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.	0:74	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.
36033281	4	62	theme	high-affinity	510:522	arg1	proteins					531:538	high-affinity saliva proteins	510:538	high-affinity saliva proteins in C3H/HeN compared to BALB/cA	510:569	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	7	63	theme	amino	1028:1032	arg1	acid					1034:1037	one different amino acid	1014:1037	one different amino acid	1014:1037	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	2	64	theme	potential	208:216	arg1	reason					218:223	One potential reason	204:223	One potential reason	204:223	One potential reason seems to be a difference in pellicle-forming saliva protein composition.
36033281	2	64	theme	potential	208:216	arg1	difference					239:248	a difference	237:248	a difference in pellicle-forming saliva protein composition	237:295	One potential reason seems to be a difference in pellicle-forming saliva protein composition.
36033281	6	65	theme	western	770:776	arg1	blotting					778:785	western blotting	770:785	western blotting using a specific polyclonal antibody	770:822	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	0	66	theme	saliva	41:46	arg1	proteins					48:55	high affinity mouse saliva proteins	21:55	high affinity mouse saliva proteins	21:55	Proteome analysis of high affinity mouse saliva proteins to hydroxyapatite.
36033281	7	67	from	glands	853:858	arg1	amplification					887:899	PCR amplification	883:899	PCR amplification of cDNA with a CAVI specific primer	883:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	7	67	from	glands	853:858	arg1	analysis					951:958	sequence analysis	942:958	sequence analysis	942:958	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	7	67	from	glands	853:858	arg1	RNA					831:833	Total RNA	825:833	Total RNA from the salivary glands of both mouse strains	825:880	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	9	68	theme	CAVI	1114:1117	arg1	activity					1119:1126	CAVI activity	1114:1126	CAVI activity	1114:1126	CAVI activity was estimated from distinct circular dichroism spectra of the molecules and found higher in C3H/HeN mice.
36033281	4	69	theme	proteins	531:538	arg1	quantity					498:505	the quantity	494:505	the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA	494:569	HAP column chromatography revealed twice the quantity of high-affinity saliva proteins in C3H/HeN compared to BALB/cA.
36033281	8	70	theme	mass	1099:1102	arg1	analysis					1104:1111	mass analysis	1099:1111	mass analysis	1099:1111	Glyco-chains of CAVI deviate in one N-glycan, confirmed by mass analysis.
36033281	11	71	from	mice	1394:1397	arg1	saliva					1357:1362	saliva	1357:1362	saliva from caries resistant C3H/HeN mice	1357:1397	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	7	72	theme	CAVI	916:919	arg1	primer					930:935	a CAVI specific primer	914:935	a CAVI specific primer	914:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	7	73	theme	different	1018:1026	arg1	acid					1034:1037	one different amino acid	1014:1037	one different amino acid	1014:1037	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	1	74	theme	inbred	129:134	arg1	mice					136:139	inbred mice	129:139	inbred mice	129:139	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	7	75	theme	cDNA	904:907	arg1	amplification					887:899	PCR amplification	883:899	PCR amplification of cDNA with a CAVI specific primer	883:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	7	75	theme	cDNA	904:907	arg1	analysis					951:958	sequence analysis	942:958	sequence analysis	942:958	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	7	75	theme	cDNA	904:907	arg1	RNA					831:833	Total RNA	825:833	Total RNA from the salivary glands of both mouse strains	825:880	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
36033281	11	76	theme	hydroxyapatite	1447:1460	arg1	adsorption					1462:1471	overall hydroxyapatite adsorption	1439:1471	overall hydroxyapatite adsorption	1439:1471	Further, saliva from caries resistant C3H/HeN mice displayed higher CAVI activity and also overall hydroxyapatite adsorption, suggesting a relationship with caries susceptibility.
36033281	1	77	contain	has	183:185	arg2	sensitivity					191:201	low sensitivity	187:201	low sensitivity	187:201	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	1	77	contain	has	183:185	arg1	C3H/HeN					175:181	C3H/HeN	175:181	C3H/HeN	175:181	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	6	78	theme	carbonic	691:698	arg1	anhydrase					700:708	murine carbonic anhydrase VI	684:711	murine carbonic anhydrase VI (CAVI)	684:718	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	6	78	theme	carbonic	691:698	arg1	CAVI					714:717	CAVI	714:717	CAVI	714:717	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	6	78	theme	carbonic	691:698	arg1	They					660:663	They	660:663	They	660:663	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	1	79	theme	mice	136:139	arg1	strains					118:124	the two strains	110:124	the two strains of inbred mice	110:139	Caries sensitivity varies between the two strains of inbred mice, BALB/cA has high sensitivity and C3H/HeN has low sensitivity.
36033281	3	80	theme	proteomic	319:327	arg1	analysis					329:336	a proteomic analysis	317:336	a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains	317:450	Here, we performed a proteomic analysis in order to identify differences of hydroxyapatite (HAP) adsorbed saliva proteins between these two mouse strains.
36033281	6	81	theme	mass	731:734	arg1	fingerprinting					736:749	peptide mass fingerprinting	723:749	peptide mass fingerprinting	723:749	They were identified as murine carbonic anhydrase VI (CAVI) by peptide mass fingerprinting and confirmed with western blotting using a specific polyclonal antibody.
36033281	7	82	with	analysis	951:958	arg1	primer					930:935	a CAVI specific primer	914:935	a CAVI specific primer	914:935	Total RNA from the salivary glands of both mouse strains, PCR amplification of cDNA with a CAVI specific primer, and sequence analysis revealed one different base in codon 96, resulting in one different amino acid.
35327312	4	0	theme	dry	504:506	arg1	root					516:519	dry ginseng root	504:519	dry ginseng root	504:519	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	1	1	with	carbohydrates	177:189	arg1	activities					205:214	multiple activities	196:214	multiple activities	196:214	Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities.
35327312	6	2	theme	rats	907:910	arg1	symptoms					859:866	the colitis symptoms	847:866	the colitis symptoms	847:866	Both WGP and WGPA alleviated the colitis symptoms and colon structure changes of colitis rats.
35327312	6	2	theme	rats	907:910	arg1	changes					888:894	colon structure changes	872:894	colon structure changes	872:894	Both WGP and WGPA alleviated the colitis symptoms and colon structure changes of colitis rats.
35327312	4	3	theme	acidic	567:572	arg1	fraction					574:581	acidic fraction	567:581	acidic fraction (WGPA)	567:588	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	4	3	theme	acidic	567:572	arg1	WGPA					584:587	WGPA	584:587	WGPA	584:587	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	7	4	theme	relative	1334:1341	arg1	abundance					1343:1351	the relative abundance	1330:1351	the relative abundance of Ruminococcus;	1330:1368	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	4	theme	relative	1334:1341	arg1	microbiota					1307:1316	gut microbiota	1303:1316	gut microbiota	1303:1316	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	9	5	theme	ginseng	1582:1588	arg1	polysaccharides					1590:1604	ginseng polysaccharides	1582:1604	ginseng polysaccharides	1582:1604	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	5	6	theme	animal	625:630	arg1	model					632:636	An animal model	622:636	An animal model	622:636	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	3	7	theme	effective	370:378	arg1	subfraction					380:390	its effective subfraction	366:390	its effective subfraction	366:390	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	7	8	theme	SCFAs	1397:1401	arg1	production					1383:1392	the production	1379:1392	the production of SCFAs	1379:1401	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	3	9	theme	subfraction	380:390	arg1	effects					327:333	the protective effects	312:333	the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis	312:438	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	7	10	theme	gut	1303:1305	arg1	abundance					1343:1351	the relative abundance	1330:1351	the relative abundance of Ruminococcus;	1330:1368	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	10	theme	gut	1303:1305	arg1	microbiota					1307:1316	gut microbiota	1303:1316	gut microbiota	1303:1316	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	8	11	theme	acidic	1476:1481	arg1	WGPA					1493:1496	WGPA	1493:1496	WGPA	1493:1496	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	8	11	theme	acidic	1476:1481	arg1	fraction					1483:1490	its acidic fraction	1472:1490	its acidic fraction (WGPA)	1472:1497	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	3	12	from	aim	293:295	arg1	study					283:287	this study	278:287	this study	278:287	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	5	13	theme	normal	703:708	arg1	diet					710:713	a normal diet	701:713	a normal diet (con group)	701:725	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	5	13	theme	normal	703:708	arg1	group					720:724	con group	716:724	con group	716:724	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	5	13	theme	normal	703:708	arg1	group					796:800	WGPA group	791:800	WGPA group	791:800	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	7	14	theme	microbiota	1307:1316	arg1	composition					1288:1298	composition	1288:1298	composition	1288:1298	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	14	theme	microbiota	1307:1316	arg1	diversity					1274:1282	the diversity	1270:1282	the diversity	1270:1282	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	9	15	theme	potential	1611:1619	arg1	investigation					1565:1577	investigation	1565:1577	investigation	1565:1577	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	9	15	theme	potential	1611:1619	arg1	strategy					1634:1641	a potential intervention strategy	1609:1641	a potential intervention strategy for the prevention of colitis	1609:1671	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	9	15	theme	potential	1611:1619	arg1	utilization					1549:1559	utilization	1549:1559	utilization	1549:1559	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	5	16	theme	WGP	750:752	arg1	group					754:758	WGP group	750:758	WGP group	750:758	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	5	16	theme	WGP	750:752	arg1	WGP					745:747	WGP	745:747	WGP (WGP group)	745:759	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	7	17	theme	regulated	1260:1268	arg1	diversity					1274:1282	the diversity	1270:1282	the diversity	1270:1282	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	1	18	theme	multiple	196:203	arg1	activities					205:214	multiple activities	196:214	multiple activities	196:214	Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities.
35327312	7	19	theme	intestinal	1034:1043	arg1	barrier					1045:1051	the intestinal barrier	1030:1051	the intestinal barrier	1030:1051	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	6	20	theme	structure	878:886	arg1	changes					888:894	colon structure changes	872:894	colon structure changes	872:894	Both WGP and WGPA alleviated the colitis symptoms and colon structure changes of colitis rats.
35327312	9	21	dep	utilization	1549:1559	arg1	the					1545:1547	the	1545:1547	the	1545:1547	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	0	22	theme	Protective	4:13	arg1	Effects					15:21	The Protective Effects	0:21	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.	0:128	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.
35327312	7	23	theme	activity	940:947	arg1	DAI					956:958	DAI	956:958	DAI	956:958	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	23	theme	activity	940:947	arg1	index					949:953	the disease activity index	928:953	the disease activity index (DAI) scores	928:966	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	1	24	from	ginseng	157:163	arg1	Polysaccharides					130:144	Polysaccharides	130:144	Polysaccharides from Panax ginseng	130:163	Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities.
35327312	1	24	from	ginseng	157:163	arg1	carbohydrates					177:189	natural carbohydrates	169:189	natural carbohydrates with multiple activities	169:214	Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities.
35327312	2	25	from	functions	253:261	arg1	colitis					266:272	colitis	266:272	colitis	266:272	However, little was known about its functions on colitis.
35327312	7	26	theme	reduced	995:1001	arg1	damage					1009:1014	reduced colon damage	995:1014	reduced colon damage	995:1014	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	27	theme	index	949:953	arg1	scores					961:966	the disease activity index (DAI) scores	928:966	the disease activity index (DAI) scores	928:966	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	9	28	theme	polysaccharides	1590:1604	arg1	strategy					1634:1641	a potential intervention strategy	1609:1641	a potential intervention strategy for the prevention of colitis	1609:1671	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	9	28	theme	polysaccharides	1590:1604	arg1	investigation					1565:1577	investigation	1565:1577	investigation	1565:1577	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	9	28	theme	polysaccharides	1590:1604	arg1	utilization					1549:1559	utilization	1549:1559	utilization	1549:1559	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	7	29	theme	Ruminococcus	1356:1367	arg1	abundance					1343:1351	the relative abundance	1330:1351	the relative abundance of Ruminococcus;	1330:1368	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	29	theme	Ruminococcus	1356:1367	arg1	microbiota					1307:1316	gut microbiota	1303:1316	gut microbiota	1303:1316	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	30	dep	proteins	1095:1102	arg1	cytokines					1152:1160	downregulated inflammatory cytokines	1125:1160	the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17)	1068:1191	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	30	dep	proteins	1095:1102	arg1	Occludin					1114:1121	Occludin	1114:1121	Occludin	1114:1121	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	30	dep	proteins	1095:1102	arg1	ZO-1					1105:1108	ZO-1	1105:1108	ZO-1	1105:1108	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	30	dep	proteins	1095:1102	arg1	proteins					1095:1102	the tight-junction-related proteins	1068:1102	the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17)	1068:1191	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	5	31	theme	WGPN	768:771	arg1	WGPN					762:765	WGPN	762:765	WGPN (WGPN group)	762:778	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	5	31	theme	WGPN	768:771	arg1	group					773:777	WGPN group	768:777	WGPN group	768:777	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	7	32	theme	colon	1003:1007	arg1	damage					1009:1014	reduced colon damage	995:1014	reduced colon damage	995:1014	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	4	33	dep	obtained	490:497	arg1	purified					527:534	purified	527:534	purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography	527:619	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	9	34	theme	colitis	1665:1671	arg1	prevention					1651:1660	the prevention	1647:1660	the prevention of colitis	1647:1671	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	7	35	theme	disease	932:938	arg1	DAI					956:958	DAI	956:958	DAI	956:958	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	35	theme	disease	932:938	arg1	index					949:953	the disease activity index	928:953	the disease activity index (DAI) scores	928:966	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	4	36	theme	exchange	597:604	arg1	chromatography					606:619	ion exchange chromatography	593:619	ion exchange chromatography	593:619	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	0	37	theme	Polysaccharides	34:48	arg1	Effects					15:21	The Protective Effects	0:21	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.	0:128	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.
35327312	7	38	theme	inflammatory	1139:1150	arg1	cytokines					1152:1160	downregulated inflammatory cytokines	1125:1160	the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17)	1068:1191	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	0	39	theme	Subfraction	70:80	arg1	Effects					15:21	The Protective Effects	0:21	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.	0:128	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.
35327312	4	40	theme	ginseng	455:461	arg1	polysaccharides					463:477	Water soluble ginseng polysaccharides	441:477	Water soluble ginseng polysaccharides (WGP)	441:483	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	4	40	theme	ginseng	455:461	arg1	WGP					480:482	WGP	480:482	WGP	480:482	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	7	41	theme	TLR4/MyD88/NF-κB-signaling	1211:1236	arg1	pathway					1238:1244	the TLR4/MyD88/NF-κB-signaling pathway	1207:1244	the TLR4/MyD88/NF-κB-signaling pathway in the colon	1207:1257	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	42	from	pathway	1238:1244	arg1	colon					1253:1257	the colon	1249:1257	the colon	1249:1257	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	6	43	theme	colitis	899:905	arg1	rats					907:910	colitis rats	899:910	colitis rats	899:910	Both WGP and WGPA alleviated the colitis symptoms and colon structure changes of colitis rats.
35327312	0	44	theme	Ginseng	26:32	arg1	Polysaccharides					34:48	Ginseng Polysaccharides	26:48	Ginseng Polysaccharides	26:48	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.
35327312	3	45	theme	-induced	423:430	arg1	colitis					432:438	dextran sodium sulfate (DSS)-induced colitis	395:438	dextran sodium sulfate (DSS)-induced colitis	395:438	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	6	46	theme	colitis	851:857	arg1	symptoms					859:866	the colitis symptoms	847:866	the colitis symptoms	847:866	Both WGP and WGPA alleviated the colitis symptoms and colon structure changes of colitis rats.
35327312	6	47	theme	colon	872:876	arg1	changes					888:894	colon structure changes	872:894	colon structure changes	872:894	Both WGP and WGPA alleviated the colitis symptoms and colon structure changes of colitis rats.
35327312	7	48	theme	colon	981:985	arg1	health					987:992	colon health	981:992	colon health	981:992	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	0	49	theme	Sulfate-Induced	105:119	arg1	Colitis					121:127	Dextran Sodium Sulfate-Induced Colitis	90:127	Dextran Sodium Sulfate-Induced Colitis	90:127	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.
35327312	0	50	theme	Effective	60:68	arg1	Subfraction					70:80	Their Effective Subfraction	54:80	Their Effective Subfraction	54:80	The Protective Effects of Ginseng Polysaccharides and Their Effective Subfraction against Dextran Sodium Sulfate-Induced Colitis.
35327312	7	51	theme	downregulated	1125:1137	arg1	cytokines					1152:1160	downregulated inflammatory cytokines	1125:1160	the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17)	1068:1191	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	5	52	theme	DSS	733:735	arg1	DSS					728:730	DSS	728:730	DSS (DSS group)	728:742	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	5	52	theme	DSS	733:735	arg1	group					737:741	DSS group	733:741	DSS group	733:741	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	4	53	theme	neutral	539:545	arg1	WGPN					557:560	WGPN	557:560	WGPN	557:560	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	4	53	theme	neutral	539:545	arg1	fraction					547:554	neutral fraction	539:554	neutral fraction (WGPN)	539:561	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	3	54	theme	protective	316:325	arg1	effects					327:333	the protective effects	312:333	the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis	312:438	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	3	55	from	effects	327:333	arg1	colitis					432:438	dextran sodium sulfate (DSS)-induced colitis	395:438	dextran sodium sulfate (DSS)-induced colitis	395:438	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	8	56	with	effect	1444:1449	arg1	WGPA					1493:1496	WGPA	1493:1496	WGPA	1493:1496	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	8	56	with	effect	1444:1449	arg1	fraction					1483:1490	its acidic fraction	1472:1490	its acidic fraction (WGPA)	1472:1497	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	7	57	theme	tight-junction-related	1072:1093	arg1	Occludin					1114:1121	Occludin	1114:1121	Occludin	1114:1121	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	57	theme	tight-junction-related	1072:1093	arg1	ZO-1					1105:1108	ZO-1	1105:1108	ZO-1	1105:1108	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	57	theme	tight-junction-related	1072:1093	arg1	proteins					1095:1102	the tight-junction-related proteins	1068:1102	the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17)	1068:1191	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	5	58	theme	Wistar	664:669	arg1	rats					671:674	male Wistar rats	659:674	male Wistar rats	659:674	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	7	59	dep	cytokines	1152:1160	arg1	IL-1β					1163:1167	IL-1β	1163:1167	IL-1β	1163:1167	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	5	60	theme	con	716:718	arg1	diet					710:713	a normal diet	701:713	a normal diet (con group)	701:725	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	5	60	theme	con	716:718	arg1	group					720:724	con group	716:724	con group	716:724	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	1	61	theme	Panax	151:155	arg1	ginseng					157:163	Panax ginseng	151:163	Panax ginseng	151:163	Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities.
35327312	4	62	theme	soluble	447:453	arg1	polysaccharides					463:477	Water soluble ginseng polysaccharides	441:477	Water soluble ginseng polysaccharides (WGP)	441:483	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	4	62	theme	soluble	447:453	arg1	WGP					480:482	WGP	480:482	WGP	480:482	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	5	63	theme	WGPA	791:794	arg1	diet					710:713	a normal diet	701:713	a normal diet (con group)	701:725	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	5	63	theme	WGPA	791:794	arg1	group					796:800	WGPA group	791:800	WGPA group	791:800	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	3	64	theme	ginseng	338:344	arg1	polysaccharides					346:360	ginseng polysaccharides	338:360	ginseng polysaccharides	338:360	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	7	65	dep	IL-1β	1163:1167	arg1	IL-6					1176:1179	IL-6	1176:1179	IL-6	1176:1179	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	65	dep	IL-1β	1163:1167	arg1	IL-17					1186:1190	IL-17	1186:1190	IL-17	1186:1190	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	7	65	dep	IL-1β	1163:1167	arg1	IL-2					1170:1173	IL-2	1170:1173	IL-2	1170:1173	They decreased the disease activity index (DAI) scores and improved colon health; reduced colon damage and recovered the intestinal barrier via regulating the tight-junction-related proteins (ZO-1 and Occludin); downregulated inflammatory cytokines (IL-1β, IL-2, IL-6, and IL-17) and inhibited the TLR4/MyD88/NF-κB-signaling pathway in the colon; regulated the diversity and composition of gut microbiota, especially the relative abundance of Ruminococcus; enhanced the production of SCFAs.
35327312	8	66	theme	effective	1505:1513	arg1	fraction					1515:1522	an effective fraction	1502:1522	an effective fraction	1502:1522	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	8	66	theme	effective	1505:1513	arg1	effect					1444:1449	a protective effect	1431:1449	a protective effect against colitis with its acidic fraction (WGPA)	1431:1497	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	4	67	theme	ion	593:595	arg1	chromatography					606:619	ion exchange chromatography	593:619	ion exchange chromatography	593:619	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	3	68	theme	polysaccharides	346:360	arg1	effects					327:333	the protective effects	312:333	the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis	312:438	In this study, we aim to investigate the protective effects of ginseng polysaccharides and its effective subfraction on dextran sodium sulfate (DSS)-induced colitis.
35327312	4	69	theme	ginseng	508:514	arg1	root					516:519	dry ginseng root	504:519	dry ginseng root	504:519	Water soluble ginseng polysaccharides (WGP) were obtained from dry ginseng root, then purified to neutral fraction (WGPN) and acidic fraction (WGPA) by ion exchange chromatography.
35327312	8	70	theme	protective	1433:1442	arg1	fraction					1515:1522	an effective fraction	1502:1522	an effective fraction	1502:1522	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	8	70	theme	protective	1433:1442	arg1	effect					1444:1449	a protective effect	1431:1449	a protective effect against colitis with its acidic fraction (WGPA)	1431:1497	In conclusion, WGP exerted a protective effect against colitis with its acidic fraction (WGPA) as an effective fraction.
35327312	1	71	theme	natural	169:175	arg1	Polysaccharides					130:144	Polysaccharides	130:144	Polysaccharides from Panax ginseng	130:163	Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities.
35327312	1	71	theme	natural	169:175	arg1	carbohydrates					177:189	natural carbohydrates	169:189	natural carbohydrates with multiple activities	169:214	Polysaccharides from Panax ginseng are natural carbohydrates with multiple activities.
35327312	5	72	theme	male	659:662	arg1	rats					671:674	male Wistar rats	659:674	male Wistar rats	659:674	An animal model was constructed with male Wistar rats, which were treated with a normal diet (con group), DSS (DSS group), WGP (WGP group), WGPN (WGPN group), and WGPA (WGPA group), respectively.
35327312	9	73	theme	intervention	1621:1632	arg1	investigation					1565:1577	investigation	1565:1577	investigation	1565:1577	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	9	73	theme	intervention	1621:1632	arg1	strategy					1634:1641	a potential intervention strategy	1609:1641	a potential intervention strategy for the prevention of colitis	1609:1671	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
35327312	9	73	theme	intervention	1621:1632	arg1	utilization					1549:1559	utilization	1549:1559	utilization	1549:1559	The results support the utilization and investigation of ginseng polysaccharides as a potential intervention strategy for the prevention of colitis.
36368924	5	0	from	sheep	1072:1076	arg1	different					1012:1020	different	1012:1020	different	1012:1020	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	5	0	from	sheep	1072:1076	arg1	composition					982:992	the microbial composition	968:992	the microbial composition	968:992	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	3	1	theme	male	486:489	arg1	sheep					491:495	3 wild-type male sheep	474:495	3 wild-type male sheep	474:495	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	10	2	theme	communities	2040:2050	arg1	composition					1984:1994	composition	1984:1994	composition	1984:1994	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	10	2	theme	communities	2040:2050	arg1	function					2010:2017	potential function	2000:2017	potential function	2000:2017	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	8	3	theme	adipocytokines	1608:1621	arg1	pathways					1623:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	3	4	theme	gut	515:517	arg1	microbiome					519:528	the gut microbiome	511:528	the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing	511:660	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	2	5	theme	potential	359:367	arg1	functions					369:377	potential functions	359:377	potential functions	359:377	The goal of this study was to explore how MSTN inactivation influences their gut microbiota composition and potential functions.
36368924	10	6	theme	CONCLUSIONS	1903:1913	arg1	study					1919:1923	CONCLUSIONS Our study	1903:1923	CONCLUSIONS Our study	1903:1923	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	3	7	theme	MSTN-edited	537:547	arg1	sheep					549:553	the MSTN-edited sheep	533:553	the MSTN-edited sheep (8 female and 8 male sheep)	533:581	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	3	8	dep	female	558:563	arg1	sheep					576:580	sheep	576:580	sheep	576:580	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	4	9	theme	meat	836:839	arg1	indexes					849:855	the meat quality indexes	832:855	the meat quality indexes	832:855	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	9	10	theme	MSTN-edited	1873:1883	arg1	P < 0.05					1892:1899	P < 0.05	1892:1899	P < 0.05	1892:1899	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	9	10	theme	MSTN-edited	1873:1883	arg1	sheep					1885:1889	the MSTN-edited sheep	1869:1889	the MSTN-edited sheep (P < 0.05)	1869:1900	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	2	11	theme	study	268:272	arg1	goal					255:258	The goal	251:258	The goal of this study	251:272	The goal of this study was to explore how MSTN inactivation influences their gut microbiota composition and potential functions.
36368924	0	12	from	function	69:76	arg1	sheep					103:107	sheep	103:107	sheep	103:107	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	10	13	theme	growth	2093:2098	arg1	performance					2100:2110	growth performance	2093:2110	growth performance	2093:2110	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	5	14	theme	microbial	972:980	arg1	different					1012:1020	different	1012:1020	different	1012:1020	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	5	14	theme	microbial	972:980	arg1	composition					982:992	the microbial composition	968:992	the microbial composition	968:992	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	4	15	theme	significant	805:815	arg1	differences					817:827	no significant differences	802:827	no significant differences in the meat quality indexes	802:855	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	0	16	from	composition	53:63	arg1	sheep					103:107	sheep	103:107	sheep	103:107	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	10	17	theme	meat	2130:2133	arg1	quality					2135:2141	meat quality	2130:2141	meat quality	2130:2141	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	8	18	theme	functional	1428:1437	arg1	differences					1439:1449	functional differences	1428:1449	functional differences	1428:1449	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	5	19	theme	PCoA	1025:1028	arg1	analysis					1030:1037	PCoA analysis	1025:1037	PCoA analysis	1025:1037	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	4	20	dep	weight	696:701	arg1	the					687:689	the	687:689	the	687:689	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	10	21	theme	potential	2000:2008	arg1	function					2010:2017	potential function	2000:2017	potential function	2000:2017	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	4	22	theme	body	691:694	arg1	weight					696:701	body weight	691:701	body weight	691:701	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	0	23	dep	composition	53:63	arg1	the					49:51	the	49:51	the	49:51	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	8	24	theme	carotenoid	1470:1479	arg1	biosynthesis					1481:1492	the carotenoid biosynthesis	1466:1492	the carotenoid biosynthesis	1466:1492	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	3	25	dep	RESULTS	380:386	arg1	We					388:389	We	388:389	We	388:389	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	0	26	theme	microbiome	89:98	arg1	function					69:76	function	69:76	function	69:76	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	0	26	theme	microbiome	89:98	arg1	composition					53:63	composition	53:63	composition	53:63	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	9	27	dep	GT41	1832:1835	arg1	the					1828:1830	the	1828:1830	the	1828:1830	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	5	28	theme	alpha	886:890	arg1	diversity					892:900	the alpha diversity	882:900	the alpha diversity	882:900	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	5	28	theme	alpha	886:890	arg1	higher					920:925	higher	920:925	higher	920:925	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	9	29	theme	GH13_39	1771:1777	arg1	abundance					1754:1762	certified the abundance	1740:1762	certified the abundance of the GH13_39	1740:1777	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	3	30	theme	slaughter	404:412	arg1	parameters					414:423	the slaughter parameters	400:423	the slaughter parameters	400:423	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	7	31	theme	Subdoligranulum	1247:1261	arg1	abundance					1218:1226	the abundance	1214:1226	the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus	1214:1340	At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05).
36368924	5	32	theme	microbial	865:873	arg1	level					875:879	the microbial level	861:879	the microbial level	861:879	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	3	33	theme	sheep	597:601	arg1	microbiome					519:528	the gut microbiome	511:528	the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing	511:660	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	0	34	theme	gene	25:28	arg1	expression					30:39	the MSTN gene expression	16:39	the MSTN gene expression	16:39	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	5	35	theme	MSTN-edited	1046:1056	arg1	sheep					1072:1076	the MSTN-edited and wild-type sheep	1042:1076	the MSTN-edited and wild-type sheep	1042:1076	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	4	36	theme	MSTN-edited	742:752	arg1	sheep					754:758	MSTN-edited sheep	742:758	MSTN-edited sheep	742:758	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	5	37	theme	wild-type	1062:1070	arg1	sheep					1072:1076	the MSTN-edited and wild-type sheep	1042:1076	the MSTN-edited and wild-type sheep	1042:1076	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	10	38	theme	microbial	2030:2038	arg1	communities					2040:2050	hindgut microbial communities	2022:2050	hindgut microbial communities	2022:2050	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	6	39	theme	Firmicutes	1096:1105	arg1	abundance					1083:1091	The abundance	1079:1091	The abundance of Firmicutes	1079:1105	The abundance of Firmicutes significantly increased and Bacteroidota significantly decreased in the MSTN-edited sheep.
36368924	3	40	theme	male	459:462	arg1	sheep					464:468	3 MSTN-edited male sheep	445:468	3 MSTN-edited male sheep	445:468	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	1	41	theme	deficient	197:205	arg1	sheep					207:211	MSTN deficient sheep	192:211	MSTN deficient sheep	192:211	BACKGROUND Myostatin (MSTN) negatively regulates the muscle growth in animals and MSTN deficient sheep have been widely reported previously.
36368924	7	42	theme	genus	1201:1205	arg1	level					1207:1211	genus level	1201:1211	genus level	1201:1211	At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05).
36368924	4	43	theme	eye	723:725	arg1	area					734:737	eye muscle area	723:737	eye muscle area	723:737	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	3	44	theme	sheep	491:495	arg1	parameters					414:423	the slaughter parameters	400:423	the slaughter parameters	400:423	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	3	44	theme	sheep	491:495	arg1	quality					434:440	meat quality	429:440	meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep	429:495	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	10	45	theme	sheep	2059:2063	arg1	composition					1984:1994	composition	1984:1994	composition	1984:1994	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	10	45	theme	sheep	2059:2063	arg1	function					2010:2017	potential function	2000:2017	potential function	2000:2017	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	8	46	theme	biosynthesis	1575:1586	arg1	pathways					1623:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	2	47	theme	gut	328:330	arg1	composition					343:353	their gut microbiota composition	322:353	their gut microbiota composition	322:353	The goal of this study was to explore how MSTN inactivation influences their gut microbiota composition and potential functions.
36368924	8	48	theme	thermogenesis	1589:1601	arg1	pathways					1623:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	3	49	dep	female	606:611	arg1	sheep					624:628	sheep	624:628	sheep	624:628	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	3	50	dep	sheep	549:553	arg1	female					558:563	8 female	556:563	8 female	556:563	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	3	50	dep	sheep	549:553	arg1	male					571:574	8 male	569:574	8 male	569:574	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	4	51	theme	sheep	754:758	arg1	area					734:737	eye muscle area	723:737	eye muscle area	723:737	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	4	51	theme	sheep	754:758	arg1	weight					696:701	body weight	691:701	body weight	691:701	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	4	51	theme	sheep	754:758	arg1	weight					712:717	carcass weight	704:717	carcass weight	704:717	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	7	52	theme	MSTN-edited	1378:1388	arg1	P < 0.05					1397:1404	P < 0.05	1397:1404	P < 0.05	1397:1404	At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05).
36368924	7	52	theme	MSTN-edited	1378:1388	arg1	sheep					1390:1394	the MSTN-edited sheep	1374:1394	the MSTN-edited sheep (P < 0.05)	1374:1405	At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05).
36368924	3	53	theme	metagenomic	639:649	arg1	sequencing					651:660	metagenomic sequencing	639:660	metagenomic sequencing	639:660	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	6	54	theme	MSTN-edited	1179:1189	arg1	sheep					1191:1195	the MSTN-edited sheep	1175:1195	the MSTN-edited sheep	1175:1195	The abundance of Firmicutes significantly increased and Bacteroidota significantly decreased in the MSTN-edited sheep.
36368924	1	55	theme	muscle	163:168	arg1	growth					170:175	the muscle growth	159:175	the muscle growth in animals	159:186	BACKGROUND Myostatin (MSTN) negatively regulates the muscle growth in animals and MSTN deficient sheep have been widely reported previously.
36368924	10	56	dep	composition	1984:1994	arg1	the					1980:1982	the	1980:1982	the	1980:1982	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	3	57	theme	wild-type	476:484	arg1	sheep					491:495	3 wild-type male sheep	474:495	3 wild-type male sheep	474:495	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	4	58	theme	quality	841:847	arg1	indexes					849:855	the meat quality indexes	832:855	the meat quality indexes	832:855	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	8	59	theme	Further	1408:1414	arg1	analysis					1416:1423	Further analysis	1408:1423	Further analysis of functional differences	1408:1449	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	7	60	theme	Lactobacillus	1328:1340	arg1	abundance					1218:1226	the abundance	1214:1226	the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus	1214:1340	At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05).
36368924	9	61	dep	enzymes	1714:1720	arg1	results					1732:1738	results	1732:1738	results certified the abundance of the GH13_39	1732:1777	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	3	62	theme	sheep	549:553	arg1	microbiome					519:528	the gut microbiome	511:528	the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing	511:660	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	9	63	theme	carbohydrate-active	1694:1712	arg1	GH4					1780:1782	GH4	1780:1782	GH4	1780:1782	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	9	63	theme	carbohydrate-active	1694:1712	arg1	PL17					1801:1804	PL17	1801:1804	PL17	1801:1804	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	9	63	theme	carbohydrate-active	1694:1712	arg1	enzymes					1714:1720	carbohydrate-active enzymes	1694:1720	carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39	1694:1777	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	9	63	theme	carbohydrate-active	1694:1712	arg1	GH137					1785:1789	GH137	1785:1789	GH137	1785:1789	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	9	63	theme	carbohydrate-active	1694:1712	arg1	CAZymes					1723:1729	CAZymes	1723:1729	CAZymes	1723:1729	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	9	63	theme	carbohydrate-active	1694:1712	arg1	GH71					1792:1795	GH71	1792:1795	GH71	1792:1795	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	7	64	theme	Oribacterium	1311:1322	arg1	abundance					1218:1226	the abundance	1214:1226	the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus	1214:1340	At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05).
36368924	4	65	from	differences	817:827	arg1	indexes					849:855	the meat quality indexes	832:855	the meat quality indexes	832:855	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	1	66	theme	BACKGROUND	110:119	arg1	MSTN					132:135	MSTN	132:135	MSTN	132:135	BACKGROUND Myostatin (MSTN) negatively regulates the muscle growth in animals and MSTN deficient sheep have been widely reported previously.
36368924	1	66	theme	BACKGROUND	110:119	arg1	Myostatin					121:129	BACKGROUND Myostatin	110:129	BACKGROUND Myostatin (MSTN)	110:136	BACKGROUND Myostatin (MSTN) negatively regulates the muscle growth in animals and MSTN deficient sheep have been widely reported previously.
36368924	8	67	theme	differences	1439:1449	arg1	analysis					1416:1423	Further analysis	1408:1423	Further analysis of functional differences	1408:1449	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	2	68	theme	MSTN	293:296	arg1	inactivation					298:309	MSTN inactivation	293:309	MSTN inactivation	293:309	The goal of this study was to explore how MSTN inactivation influences their gut microbiota composition and potential functions.
36368924	1	69	from	growth	170:175	arg1	animals					180:186	animals	180:186	animals	180:186	BACKGROUND Myostatin (MSTN) negatively regulates the muscle growth in animals and MSTN deficient sheep have been widely reported previously.
36368924	7	70	theme	Agathobaculum	1282:1294	arg1	abundance					1218:1226	the abundance	1214:1226	the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus	1214:1340	At genus level, the abundance of Flavonifractor, Subdoligranulum, Ruthenibacterium, Agathobaculum, Anaerotignum, Oribacterium and Lactobacillus were significantly increased in the MSTN-edited sheep (P < 0.05).
36368924	10	71	theme	MSTN	1940:1943	arg1	inactivation					1945:1956	MSTN inactivation	1940:1956	MSTN inactivation	1940:1956	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	9	72	theme	certified	1740:1748	arg1	abundance					1754:1762	certified the abundance	1740:1762	certified the abundance of the GH13_39	1740:1777	Moreover, carbohydrate-active enzymes (CAZymes) results certified the abundance of the GH13_39, GH4, GH137, GH71 and PL17 were upregulated, and the GT41 and CBM20 were downregulated in the MSTN-edited sheep (P < 0.05).
36368924	0	73	theme	gut	85:87	arg1	microbiome					89:98	the gut microbiome	81:98	the gut microbiome	81:98	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	3	74	theme	meat	429:432	arg1	quality					434:440	meat quality	429:440	meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep	429:495	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	5	75	from	different	1012:1020	arg1	sheep					1072:1076	the MSTN-edited and wild-type sheep	1042:1076	the MSTN-edited and wild-type sheep	1042:1076	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	0	76	theme	MSTN	20:23	arg1	expression					30:39	the MSTN gene expression	16:39	the MSTN gene expression	16:39	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	8	77	theme	peroxisome	1530:1539	arg1	pathways					1623:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	3	78	theme	wild-type	587:595	arg1	sheep					597:601	wild-type sheep	587:601	wild-type sheep (8 female and 8 male sheep)	587:629	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	3	79	theme	MSTN-edited	447:457	arg1	sheep					464:468	3 MSTN-edited male sheep	445:468	3 MSTN-edited male sheep	445:468	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	0	80	theme	expression	30:39	arg1	Inactivation					0:11	Inactivation	0:11	Inactivation of the MSTN gene expression	0:39	Inactivation of the MSTN gene expression changes the composition and function of the gut microbiome in sheep.
36368924	8	81	theme	apoptosis	1542:1550	arg1	pathways					1623:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	4	82	theme	muscle	727:732	arg1	area					734:737	eye muscle area	723:737	eye muscle area	723:737	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	5	83	theme	MSTN-edited	934:944	arg1	P < 0.05					953:960	P < 0.05	953:960	P < 0.05	953:960	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	5	83	theme	MSTN-edited	934:944	arg1	sheep					946:950	the MSTN-edited sheep	930:950	the MSTN-edited sheep (P < 0.05)	930:961	At the microbial level, the alpha diversity was significantly higher in the MSTN-edited sheep (P < 0.05), and the microbial composition was significantly different by PCoA analysis in the MSTN-edited and wild-type sheep.
36368924	8	84	theme	ferroptosis	1553:1563	arg1	pathways					1623:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways	1526:1630	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	10	85	theme	hindgut	2022:2028	arg1	communities					2040:2050	hindgut microbial communities	2022:2050	hindgut microbial communities	2022:2050	CONCLUSIONS Our study suggested that MSTN inactivation remarkably influenced the composition and potential function of hindgut microbial communities of the sheep, and significantly promoted growth performance without affecting meat quality.
36368924	8	86	theme	N-glycan	1566:1573	arg1	biosynthesis					1575:1586	N-glycan biosynthesis	1566:1586	N-glycan biosynthesis	1566:1586	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	4	87	theme	carcass	704:710	arg1	weight					712:717	carcass weight	704:717	carcass weight	704:717	The results showed that the body weight, carcass weight and eye muscle area of MSTN-edited sheep were significantly higher, but there were no significant differences in the meat quality indexes.
36368924	3	88	theme	sheep	464:468	arg1	parameters					414:423	the slaughter parameters	400:423	the slaughter parameters	400:423	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	3	88	theme	sheep	464:468	arg1	quality					434:440	meat quality	429:440	meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep	429:495	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	2	89	theme	microbiota	332:341	arg1	composition					343:353	their gut microbiota composition	322:353	their gut microbiota composition	322:353	The goal of this study was to explore how MSTN inactivation influences their gut microbiota composition and potential functions.
36368924	8	90	theme	MSTN-edited	1654:1664	arg1	P < 0.05					1673:1680	P < 0.05	1673:1680	P < 0.05	1673:1680	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	8	90	theme	MSTN-edited	1654:1664	arg1	sheep					1666:1670	the MSTN-edited sheep	1650:1670	the MSTN-edited sheep (P < 0.05)	1650:1681	Further analysis of functional differences was found that the carotenoid biosynthesis was significantly increased and the peroxisome, apoptosis, ferroptosis, N-glycan biosynthesis, thermogenesis, and adipocytokines pathways were decreased in the MSTN-edited sheep (P < 0.05).
36368924	3	91	dep	sheep	597:601	arg1	female					606:611	8 female	604:611	8 female	604:611	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
36368924	3	91	dep	sheep	597:601	arg1	male					619:622	8 male	617:622	8 male	617:622	RESULTS We compared the slaughter parameters and meat quality of 3 MSTN-edited male sheep and 3 wild-type male sheep, and analyzed the gut microbiome of the MSTN-edited sheep (8 female and 8 male sheep) and wild-type sheep (8 female and 8 male sheep) through metagenomic sequencing.
35406277	0	0	theme	Ascorbic	88:95	arg1	Acid					97:100	Ascorbic Acid	88:100	Ascorbic Acid	88:100	Structural Characterization of Degraded Lycium barbarum L. Leaves' Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide.
35406277	6	1	theme	degradation	926:936	arg1	effect					938:943	the degradation effect	922:943	the degradation effect	922:943	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	7	2	from	sugar	1332:1336	arg1	rich					1324:1327	rich	1324:1327	rich	1324:1327	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	1	3	theme	chain	173:177	arg1	structure					179:187	chain structure	173:187	chain structure	173:187	Plant-derived polysaccharide's conformation and chain structure play a key role in their various biological activities.
35406277	8	4	from	changes	1587:1593	arg1	structure					1628:1636	the polysaccharides molecular structure	1598:1636	the polysaccharides molecular structure caused by degradation	1598:1658	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	5	5	theme	Ascorbic	804:811	arg1	C					801:801	vitamin C	793:801	vitamin C (Ascorbic acid)	793:817	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	5	theme	Ascorbic	804:811	arg1	acid					813:816	Ascorbic acid	804:816	Ascorbic acid	804:816	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	6	6	theme	mass	1027:1030	arg1	ICP-MS					1046:1051	ICP-MS	1046:1051	ICP-MS	1046:1051	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	6	theme	mass	1027:1030	arg1	spectrometry					1032:1043	inductively coupled plasma mass spectrometry	1000:1043	inductively coupled plasma mass spectrometry (ICP-MS)	1000:1052	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	5	7	mod	modified	779:786	arg3	H2O2					842:845	H2O2	842:845	H2O2	842:845	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	7	mod	modified	779:786	arg1	polysaccharide					692:705	' polysaccharide	690:705	' polysaccharide	690:705	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	7	mod	modified	779:786	arg3	acid					813:816	Ascorbic acid	804:816	Ascorbic acid	804:816	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	7	mod	modified	779:786	arg3	C					801:801	vitamin C	793:801	vitamin C (Ascorbic acid)	793:817	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	7	mod	modified	779:786	arg3	peroxide					832:839	hydrogen peroxide	823:839	hydrogen peroxide (H2O2)	823:846	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	4	8	theme	polysaccharides	524:538	arg1	bioactivities					503:515	the functional bioactivities	488:515	the functional bioactivities of the polysaccharides	488:538	To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation.
35406277	7	9	theme	significant	1361:1371	arg1	effect					1411:1416	no significant major functional group transformation effect	1358:1416	no significant major functional group transformation effect	1358:1416	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	6	10	theme	performance	1188:1198	arg1	chromatography					1215:1228	high performance gel permeation chromatography	1183:1228	high performance gel permeation chromatography (HPGPC)	1183:1236	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	10	theme	performance	1188:1198	arg1	HPGPC					1231:1235	HPGPC	1231:1235	HPGPC	1231:1235	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	11	theme	chromatography-mass	1059:1077	arg1	spectrometry					1079:1090	gas chromatography-mass spectrometry	1055:1090	gas chromatography-mass spectrometry (GC-MS)	1055:1098	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	11	theme	chromatography-mass	1059:1077	arg1	GC-MS					1093:1097	GC-MS	1093:1097	GC-MS	1093:1097	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	5	12	contain	containing	648:657	arg1	extract					640:646	the ethanol extract	628:646	the ethanol extract containing crude Lycium barbarum L.	628:682	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	12	contain	containing	648:657	arg2	L.					681:682	crude Lycium barbarum L.	659:682	crude Lycium barbarum L.	659:682	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	13	dep	extracted	717:725	arg1	modified					779:786	modified	779:786	modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide	779:908	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	6	14	theme	high	1183:1186	arg1	chromatography					1215:1228	high performance gel permeation chromatography	1183:1228	high performance gel permeation chromatography (HPGPC)	1183:1236	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	14	theme	high	1183:1186	arg1	HPGPC					1231:1235	HPGPC	1231:1235	HPGPC	1231:1235	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	15	theme	infrared	1119:1126	arg1	spectroscopy					1128:1139	infrared spectroscopy	1119:1139	infrared spectroscopy (FTIR)	1119:1146	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	15	theme	infrared	1119:1126	arg1	FTIR					1142:1145	FTIR	1142:1145	FTIR	1142:1145	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	16	theme	coupled	1012:1018	arg1	ICP-MS					1046:1051	ICP-MS	1046:1051	ICP-MS	1046:1051	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	16	theme	coupled	1012:1018	arg1	spectrometry					1032:1043	inductively coupled plasma mass spectrometry	1000:1043	inductively coupled plasma mass spectrometry (ICP-MS)	1000:1052	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	8	17	theme	223.5	1523:1527	arg1	kDa					1529:1531	kDa	1529:1531	kDa	1529:1531	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	5	18	theme	ethanol	632:638	arg1	extract					640:646	the ethanol extract	628:646	the ethanol extract containing crude Lycium barbarum L.	628:682	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	7	19	theme	degraded	1425:1432	arg1	composition					1442:1452	the degraded product composition	1421:1452	the degraded product composition	1421:1452	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	7	20	from	degradation	1342:1352	arg1	rich					1324:1327	rich	1324:1327	rich	1324:1327	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	0	21	theme	Hydrogen	106:113	arg1	Peroxide					115:122	Hydrogen Peroxide	106:122	Hydrogen Peroxide	106:122	Structural Characterization of Degraded Lycium barbarum L. Leaves' Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide.
35406277	8	22	theme	64.3	1536:1539	arg1	kDa					1529:1531	kDa	1529:1531	kDa	1529:1531	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	4	23	used	used	564:567	arg2	degradation					541:551	degradation	541:551	degradation	541:551	To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation.
35406277	0	24	theme	Structural	0:9	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization of Degraded Lycium barbarum L.	0:57	Structural Characterization of Degraded Lycium barbarum L. Leaves' Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide.
35406277	3	25	theme	compact	396:402	arg1	structure					413:421	compact globular structure	396:421	compact globular structure	396:421	However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight.
35406277	4	26	theme	functional	492:501	arg1	bioactivities					503:515	the functional bioactivities	488:515	the functional bioactivities of the polysaccharides	488:538	To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation.
35406277	6	27	theme	electron	1252:1259	arg1	microscope					1261:1270	scanning electron microscope	1243:1270	scanning electron microscope (SEM)	1243:1276	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	27	theme	electron	1252:1259	arg1	SEM					1273:1275	SEM	1273:1275	SEM	1273:1275	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	7	28	theme	product	1434:1440	arg1	composition					1442:1452	the degraded product composition	1421:1452	the degraded product composition	1421:1452	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	8	29	theme	molecular	1618:1626	arg1	structure					1628:1636	the polysaccharides molecular structure	1598:1636	the polysaccharides molecular structure caused by degradation	1598:1658	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	1	30	theme	key	196:198	arg1	role					200:203	a key role	194:203	a key role	194:203	Plant-derived polysaccharide's conformation and chain structure play a key role in their various biological activities.
35406277	3	31	theme	globular	404:411	arg1	structure					413:421	compact globular structure	396:421	compact globular structure	396:421	However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight.
35406277	6	32	theme	scanning	1243:1250	arg1	microscope					1261:1270	scanning electron microscope	1243:1270	scanning electron microscope (SEM)	1243:1276	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	32	theme	scanning	1243:1250	arg1	SEM					1273:1275	SEM	1273:1275	SEM	1273:1275	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	0	33	dep	Using	82:86	arg1	Polysaccharide					67:80	Polysaccharide	67:80	Polysaccharide	67:80	Structural Characterization of Degraded Lycium barbarum L. Leaves' Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide.
35406277	7	34	theme	major	1373:1377	arg1	effect					1411:1416	no significant major functional group transformation effect	1358:1416	no significant major functional group transformation effect	1358:1416	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	2	35	theme	health	312:317	arg1	functions					319:327	its health functions	308:327	its health functions	308:327	Lycium barbarum L. leaves' polysaccharide is well renowned for its health functions.
35406277	7	36	theme	functional	1379:1388	arg1	effect					1411:1416	no significant major functional group transformation effect	1358:1416	no significant major functional group transformation effect	1358:1416	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	4	37	theme	polysaccharides	583:597	arg1	conformation					599:610	the polysaccharides conformation	579:610	the polysaccharides conformation	579:610	To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation.
35406277	6	38	theme	magnetic	1157:1164	arg1	NMR					1177:1179	NMR	1177:1179	NMR	1177:1179	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	38	theme	magnetic	1157:1164	arg1	resonance					1166:1174	nuclear magnetic resonance	1149:1174	nuclear magnetic resonance (NMR)	1149:1180	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	3	39	theme	high	427:430	arg1	weight					442:447	high molecular weight	427:447	high molecular weight	427:447	However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight.
35406277	4	40	theme	such	462:465	arg1	issue					467:471	such issue	462:471	such issue	462:471	To overcome such issue and to improve the functional bioactivities of the polysaccharides, degradation is usually used to modify the polysaccharides conformation.
35406277	7	41	theme	transformation	1396:1409	arg1	effect					1411:1416	no significant major functional group transformation effect	1358:1416	no significant major functional group transformation effect	1358:1416	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	1	42	theme	various	214:220	arg1	activities					233:242	their various biological activities	208:242	their various biological activities	208:242	Plant-derived polysaccharide's conformation and chain structure play a key role in their various biological activities.
35406277	0	43	theme	Lycium	40:45	arg1	Characterization					11:26	Structural Characterization	0:26	Structural Characterization of Degraded Lycium barbarum L.	0:57	Structural Characterization of Degraded Lycium barbarum L. Leaves' Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide.
35406277	8	44	theme	significant	1575:1585	arg1	changes					1587:1593	significant changes	1575:1593	significant changes in the polysaccharides molecular structure caused by degradation	1575:1658	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	3	45	theme	molecular	432:440	arg1	weight					442:447	high molecular weight	427:447	high molecular weight	427:447	However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight.
35406277	8	46	theme	molecular	1468:1476	arg1	Mw					1486:1487	Mw	1486:1487	Mw	1486:1487	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	8	46	theme	molecular	1468:1476	arg1	weight					1478:1483	the molecular weight	1464:1483	the molecular weight (Mw)	1464:1488	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	7	47	theme	group	1390:1394	arg1	effect					1411:1416	no significant major functional group transformation effect	1358:1416	no significant major functional group transformation effect	1358:1416	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	1	48	theme	biological	222:231	arg1	activities					233:242	their various biological activities	208:242	their various biological activities	208:242	Plant-derived polysaccharide's conformation and chain structure play a key role in their various biological activities.
35406277	6	49	theme	gas	1055:1057	arg1	spectrometry					1079:1090	gas chromatography-mass spectrometry	1055:1090	gas chromatography-mass spectrometry (GC-MS)	1055:1098	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	49	theme	gas	1055:1057	arg1	GC-MS					1093:1097	GC-MS	1093:1097	GC-MS	1093:1097	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	5	50	theme	vitamin	793:799	arg1	C					801:801	vitamin C	793:801	vitamin C (Ascorbic acid)	793:817	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	50	theme	vitamin	793:799	arg1	acid					813:816	Ascorbic acid	804:816	Ascorbic acid	804:816	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	51	dep	Lycium	868:873	arg1	L.					884:885	Lycium barbarum L.	868:885	Lycium barbarum L.	868:885	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	6	52	theme	nuclear	1149:1155	arg1	NMR					1177:1179	NMR	1177:1179	NMR	1177:1179	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	52	theme	nuclear	1149:1155	arg1	resonance					1166:1174	nuclear magnetic resonance	1149:1174	nuclear magnetic resonance (NMR)	1149:1180	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	0	53	dep	Lycium	40:45	arg1	L.					56:57	Lycium barbarum L.	40:57	Degraded Lycium barbarum L.	31:57	Structural Characterization of Degraded Lycium barbarum L. Leaves' Polysaccharide Using Ascorbic Acid and Hydrogen Peroxide.
35406277	1	54	theme	Plant-derived	125:137	arg1	conformation					156:167	Plant-derived polysaccharide's conformation	125:167	Plant-derived polysaccharide's conformation	125:167	Plant-derived polysaccharide's conformation and chain structure play a key role in their various biological activities.
35406277	5	55	dep	produce	851:857	arg1	degraded					859:866	degraded	859:866	to produce degraded Lycium barbarum L. leaves' polysaccharide	848:908	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	6	56	dep	Fourier	1101:1107	arg1	transform					1109:1117	transform	1109:1117	transform infrared spectroscopy (FTIR)	1109:1146	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	57	theme	plasma	1020:1025	arg1	ICP-MS					1046:1051	ICP-MS	1046:1051	ICP-MS	1046:1051	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	57	theme	plasma	1020:1025	arg1	spectrometry					1032:1043	inductively coupled plasma mass spectrometry	1000:1043	inductively coupled plasma mass spectrometry (ICP-MS)	1000:1052	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	1	58	link	Plant-derived	125:137	arg1	conformation					156:167	Plant-derived polysaccharide's conformation	125:167	Plant-derived polysaccharide's conformation	125:167	Plant-derived polysaccharide's conformation and chain structure play a key role in their various biological activities.
35406277	8	59	theme	polysaccharides	1602:1616	arg1	structure					1628:1636	the polysaccharides molecular structure	1598:1636	the polysaccharides molecular structure caused by degradation	1598:1658	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	2	60	dep	Lycium	245:250	arg1	leaves					264:269	leaves	264:269	leaves	264:269	Lycium barbarum L. leaves' polysaccharide is well renowned for its health functions.
35406277	2	60	dep	Lycium	245:250	arg1	L.					261:262	Lycium barbarum L.	245:262	Lycium barbarum L. leaves' polysaccharide	245:285	Lycium barbarum L. leaves' polysaccharide is well renowned for its health functions.
35406277	6	61	theme	permeation	1204:1213	arg1	chromatography					1215:1228	high performance gel permeation chromatography	1183:1228	high performance gel permeation chromatography (HPGPC)	1183:1236	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	61	theme	permeation	1204:1213	arg1	HPGPC					1231:1235	HPGPC	1231:1235	HPGPC	1231:1235	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	7	62	from	rich	1324:1327	arg1	degradation					1342:1352	degradation	1342:1352	degradation	1342:1352	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	7	62	from	rich	1324:1327	arg1	sugar					1332:1336	sugar	1332:1336	sugar	1332:1336	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	8	63	dep	kDa	1529:1531	arg1	to					1533:1534	to	1533:1534	to	1533:1534	However, the molecular weight (Mw) had decreased significantly from 223.5 kDa to 64.3 kDa after degradation, indicating significant changes in the polysaccharides molecular structure caused by degradation.
35406277	3	64	theme	functional	343:352	arg1	bioactivities					354:366	its functional bioactivities	339:366	its functional bioactivities	339:366	However, its functional bioactivities are greatly hindered by its compact globular structure and high molecular weight.
35406277	6	65	theme	gel	1200:1202	arg1	chromatography					1215:1228	high performance gel permeation chromatography	1183:1228	high performance gel permeation chromatography (HPGPC)	1183:1236	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	6	65	theme	gel	1200:1202	arg1	HPGPC					1231:1235	HPGPC	1231:1235	HPGPC	1231:1235	To explore the degradation effect, both polysaccharides were further characterized using inductively coupled plasma mass spectrometry (ICP-MS), gas chromatography-mass spectrometry (GC-MS), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), high performance gel permeation chromatography (HPGPC), and scanning electron microscope (SEM).
35406277	5	66	theme	hydrogen	823:830	arg1	peroxide					832:839	hydrogen peroxide	823:839	hydrogen peroxide (H2O2)	823:846	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	5	66	theme	hydrogen	823:830	arg1	H2O2					842:845	H2O2	842:845	H2O2	842:845	In this study, the ethanol extract containing crude Lycium barbarum L. leaves' polysaccharide was first extracted, further characterized, and subsequently chemically modified with vitamin C (Ascorbic acid) and hydrogen peroxide (H2O2) to produce degraded Lycium barbarum L. leaves' polysaccharide.
35406277	7	67	contain	had	1354:1356	arg2	effect					1411:1416	no significant major functional group transformation effect	1358:1416	no significant major functional group transformation effect	1358:1416	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
35406277	7	67	contain	had	1354:1356	arg1	Results					1279:1285	Results	1279:1285	Results shown that both polysaccharides were rich in sugar and degradation	1279:1352	Results shown that both polysaccharides were rich in sugar and degradation had no significant major functional group transformation effect on the degraded product composition.
36139146	0	0	theme	Lung	88:91	arg1	Injury					93:98	Acute Lung Injury	82:98	Acute Lung Injury	82:98	Aerosol-Administered Adelmidrol Attenuates Lung Inflammation in a Murine Model of Acute Lung Injury.
36139146	3	1	theme	endogenous	649:658	arg1	defense					672:678	endogenous antioxidant defense	649:678	endogenous antioxidant defense	649:678	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	4	2	theme	intratracheal	783:795	arg1	administration					797:810	intratracheal administration	783:810	intratracheal administration of lipopolysaccharide (LPS)	783:838	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	1	3	theme	common	130:135	arg1	disorder					162:169	a common and devastating clinical disorder	128:169	a common and devastating clinical disorder with a high mortality rate and no specific therapy	128:220	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	3	theme	common	130:135	arg1	injury					112:117	Acute lung injury	101:117	Acute lung injury (ALI)	101:123	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	9	4	from	candidate	1420:1428	arg1	management					1437:1446	the management	1433:1446	the management of ALI	1433:1453	These results suggest that ADM could be a potential candidate in the management of ALI.
36139146	0	5	theme	Acute	82:86	arg1	Injury					93:98	Acute Lung Injury	82:98	Acute Lung Injury	82:98	Aerosol-Administered Adelmidrol Attenuates Lung Inflammation in a Murine Model of Acute Lung Injury.
36139146	7	6	from	increase	1093:1100	arg1	number					1108:1113	MC number	1105:1113	MC number	1105:1113	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	8	7	theme	oxidative	1222:1230	arg1	stress					1232:1237	oxidative stress	1222:1237	oxidative stress	1222:1237	Moreover, ADM reduced oxidative stress by upregulating antioxidant enzymes as well as modulating the Nf-kB pathway and the resulting pro-inflammatory cytokine release.
36139146	4	8	theme	mg/kg	857:861	arg1	dose					847:850	the dose	843:850	the dose of 5 mg/kg	843:861	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	8	9	theme	cytokine	1350:1357	arg1	release					1359:1365	the resulting pro-inflammatory cytokine release	1319:1365	the resulting pro-inflammatory cytokine release	1319:1365	Moreover, ADM reduced oxidative stress by upregulating antioxidant enzymes as well as modulating the Nf-kB pathway and the resulting pro-inflammatory cytokine release.
36139146	2	10	theme	increased	270:278	arg1	permeability					299:310	increased alveolar/capillary permeability	270:310	increased alveolar/capillary permeability	270:310	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	0	11	theme	Injury	93:98	arg1	Model					73:77	a Murine Model	64:77	a Murine Model of Acute Lung Injury	64:98	Aerosol-Administered Adelmidrol Attenuates Lung Inflammation in a Murine Model of Acute Lung Injury.
36139146	0	12	theme	Aerosol-Administered	0:19	arg1	Adelmidrol					21:30	Aerosol-Administered Adelmidrol	0:30	Aerosol-Administered Adelmidrol	0:30	Aerosol-Administered Adelmidrol Attenuates Lung Inflammation in a Murine Model of Acute Lung Injury.
36139146	6	13	theme	lung	989:992	arg1	damage					994:999	lung damage	989:999	lung damage	989:999	In this study, we clearly demonstrated that ADM reduced lung damage and airway infiltration induced by LPS instillation.
36139146	7	14	theme	same	1061:1064	arg1	time					1066:1069	the same time	1057:1069	the same time	1057:1069	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	6	15	theme	airway	1005:1010	arg1	infiltration					1012:1023	airway infiltration	1005:1023	airway infiltration	1005:1023	In this study, we clearly demonstrated that ADM reduced lung damage and airway infiltration induced by LPS instillation.
36139146	4	16	theme	ALI	767:769	arg1	model					758:762	a mice model	751:762	a mice model	751:762	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	3	17	theme	palmitoylethanolamide	487:507	arg1	Adelmidrol					454:463	Adelmidrol	454:463	Adelmidrol (ADM)	454:469	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	3	17	theme	palmitoylethanolamide	487:507	arg1	analogue					475:482	an analogue	472:482	an analogue of palmitoylethanolamide (PEA)	472:513	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	1	18	theme	devastating	141:151	arg1	disorder					162:169	a common and devastating clinical disorder	128:169	a common and devastating clinical disorder with a high mortality rate and no specific therapy	128:220	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	18	theme	devastating	141:151	arg1	injury					112:117	Acute lung injury	101:117	Acute lung injury (ALI)	101:123	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	3	19	theme	antioxidant	660:670	arg1	defense					672:678	endogenous antioxidant defense	649:678	endogenous antioxidant defense	649:678	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	7	20	from	expression	1123:1132	arg1	number					1108:1113	MC number	1105:1113	MC number	1105:1113	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	2	21	theme	ALI	246:248	arg1	pathophysiology					227:241	The pathophysiology	223:241	The pathophysiology of ALI	223:248	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	1	22	theme	clinical	153:160	arg1	disorder					162:169	a common and devastating clinical disorder	128:169	a common and devastating clinical disorder with a high mortality rate and no specific therapy	128:220	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	22	theme	clinical	153:160	arg1	injury					112:117	Acute lung injury	101:117	Acute lung injury (ALI)	101:123	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	6	23	theme	LPS	1036:1038	arg1	instillation					1040:1051	LPS instillation	1036:1051	LPS instillation	1036:1051	In this study, we clearly demonstrated that ADM reduced lung damage and airway infiltration induced by LPS instillation.
36139146	2	24	theme	lung	374:377	arg1	tissues					379:385	lung tissues	374:385	lung tissues	374:385	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	2	25	theme	distress	428:435	arg1	ARDS					447:450	ARDS	447:450	ARDS	447:450	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	2	25	theme	distress	428:435	arg1	syndrome					437:444	acute respiratory distress syndrome	410:444	acute respiratory distress syndrome (ARDS)	410:451	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	8	26	theme	pro-inflammatory	1333:1348	arg1	release					1359:1365	the resulting pro-inflammatory cytokine release	1319:1365	the resulting pro-inflammatory cytokine release	1319:1365	Moreover, ADM reduced oxidative stress by upregulating antioxidant enzymes as well as modulating the Nf-kB pathway and the resulting pro-inflammatory cytokine release.
36139146	8	27	theme	resulting	1323:1331	arg1	release					1359:1365	the resulting pro-inflammatory cytokine release	1319:1365	the resulting pro-inflammatory cytokine release	1319:1365	Moreover, ADM reduced oxidative stress by upregulating antioxidant enzymes as well as modulating the Nf-kB pathway and the resulting pro-inflammatory cytokine release.
36139146	5	28	dep	h	907:907	arg1	instillation					919:930	LPS instillation	915:930	LPS instillation	915:930	ADM 2% was administered by aerosol 1 and 6 h after LPS instillation.
36139146	0	29	theme	Lung	43:46	arg1	Inflammation					48:59	Lung Inflammation	43:59	Lung Inflammation	43:59	Aerosol-Administered Adelmidrol Attenuates Lung Inflammation in a Murine Model of Acute Lung Injury.
36139146	4	30	theme	mice	753:756	arg1	model					758:762	a mice model	751:762	a mice model	751:762	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	1	31	theme	Acute	101:105	arg1	ALI					120:122	ALI	120:122	ALI	120:122	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	31	theme	Acute	101:105	arg1	disorder					162:169	a common and devastating clinical disorder	128:169	a common and devastating clinical disorder with a high mortality rate and no specific therapy	128:220	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	31	theme	Acute	101:105	arg1	injury					112:117	Acute lung injury	101:117	Acute lung injury (ALI)	101:123	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	7	32	theme	activation	1160:1169	arg1	markers					1146:1152	specific markers	1137:1152	specific markers	1137:1152	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	2	33	theme	acute	410:414	arg1	ARDS					447:450	ARDS	447:450	ARDS	447:450	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	2	33	theme	acute	410:414	arg1	syndrome					437:444	acute respiratory distress syndrome	410:444	acute respiratory distress syndrome (ARDS)	410:451	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	9	34	theme	potential	1410:1418	arg1	candidate					1420:1428	a potential candidate	1408:1428	a potential candidate	1408:1428	These results suggest that ADM could be a potential candidate in the management of ALI.
36139146	9	34	theme	potential	1410:1418	arg1	ADM					1395:1397	ADM	1395:1397	ADM	1395:1397	These results suggest that ADM could be a potential candidate in the management of ALI.
36139146	8	35	theme	Nf-kB	1301:1305	arg1	pathway					1307:1313	the Nf-kB pathway	1297:1313	the Nf-kB pathway	1297:1313	Moreover, ADM reduced oxidative stress by upregulating antioxidant enzymes as well as modulating the Nf-kB pathway and the resulting pro-inflammatory cytokine release.
36139146	1	36	theme	lung	107:110	arg1	ALI					120:122	ALI	120:122	ALI	120:122	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	36	theme	lung	107:110	arg1	disorder					162:169	a common and devastating clinical disorder	128:169	a common and devastating clinical disorder with a high mortality rate and no specific therapy	128:220	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	36	theme	lung	107:110	arg1	injury					112:117	Acute lung injury	101:117	Acute lung injury (ALI)	101:123	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	37	theme	high	178:181	arg1	rate					193:196	a high mortality rate	176:196	a high mortality rate	176:196	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	9	38	theme	ALI	1451:1453	arg1	management					1437:1446	the management	1433:1446	the management of ALI	1433:1453	These results suggest that ADM could be a potential candidate in the management of ALI.
36139146	1	39	theme	mortality	183:191	arg1	rate					193:196	a high mortality rate	176:196	a high mortality rate	176:196	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	8	40	theme	antioxidant	1255:1265	arg1	enzymes					1267:1273	antioxidant enzymes	1255:1273	antioxidant enzymes	1255:1273	Moreover, ADM reduced oxidative stress by upregulating antioxidant enzymes as well as modulating the Nf-kB pathway and the resulting pro-inflammatory cytokine release.
36139146	2	41	theme	respiratory	416:426	arg1	ARDS					447:450	ARDS	447:450	ARDS	447:450	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	2	41	theme	respiratory	416:426	arg1	syndrome					437:444	acute respiratory distress syndrome	410:444	acute respiratory distress syndrome (ARDS)	410:451	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	4	42	theme	study	697:701	arg1	aim					685:687	The aim	681:687	The aim of this study	681:701	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	0	43	theme	Murine	66:71	arg1	Model					73:77	a Murine Model	64:77	a Murine Model of Acute Lung Injury	64:98	Aerosol-Administered Adelmidrol Attenuates Lung Inflammation in a Murine Model of Acute Lung Injury.
36139146	1	44	with	disorder	162:169	arg1	therapy					214:220	no specific therapy	202:220	no specific therapy	202:220	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	1	44	with	disorder	162:169	arg1	rate					193:196	a high mortality rate	176:196	a high mortality rate	176:196	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	7	45	dep	chymase	1178:1184	arg1	i.e.					1172:1175	i.e.	1172:1175	i.e.	1172:1175	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	2	46	theme	oxidative	332:340	arg1	stress					342:347	oxidative stress	332:347	oxidative stress	332:347	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	4	47	theme	lipopolysaccharide	815:832	arg1	administration					797:810	intratracheal administration	783:810	intratracheal administration of lipopolysaccharide (LPS)	783:838	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	5	48	theme	LPS	915:917	arg1	instillation					919:930	LPS instillation	915:930	LPS instillation	915:930	ADM 2% was administered by aerosol 1 and 6 h after LPS instillation.
36139146	3	49	theme	anti-inflammatory	533:549	arg1	functions					567:575	its anti-inflammatory and antioxidant functions	529:575	its anti-inflammatory and antioxidant functions	529:575	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	7	50	theme	specific	1137:1144	arg1	markers					1146:1152	specific markers	1137:1152	specific markers	1137:1152	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	7	51	theme	markers	1146:1152	arg1	increase					1093:1100	the increase	1089:1100	the increase in MC number	1089:1113	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	7	51	theme	markers	1146:1152	arg1	expression					1123:1132	the expression	1119:1132	the expression of specific markers of MC activation, i.e., chymase and tryptase	1119:1197	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	3	52	theme	antioxidant	555:565	arg1	functions					567:575	its anti-inflammatory and antioxidant functions	529:575	its anti-inflammatory and antioxidant functions	529:575	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	2	53	theme	alveolar/capillary	280:297	arg1	permeability					299:310	increased alveolar/capillary permeability	270:310	increased alveolar/capillary permeability	270:310	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	3	54	theme	mast	618:621	arg1	cells					623:627	mast cells	618:627	mast cells (MCs)	618:633	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	3	54	theme	mast	618:621	arg1	MCs					630:632	MCs	630:632	MCs	630:632	Adelmidrol (ADM), an analogue of palmitoylethanolamide (PEA), is known for its anti-inflammatory and antioxidant functions, which are mainly due to down-modulating mast cells (MCs) and promoting endogenous antioxidant defense.
36139146	4	55	theme	protective	722:731	arg1	effects					733:739	the protective effects	718:739	the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg	718:861	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	2	56	theme	lung	313:316	arg1	inflammation					318:329	lung inflammation	313:329	lung inflammation	313:329	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	7	57	theme	MC	1157:1158	arg1	activation					1160:1169	MC activation	1157:1169	MC activation	1157:1169	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
36139146	4	58	theme	ADM	744:746	arg1	effects					733:739	the protective effects	718:739	the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg	718:861	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	5	59	theme	ADM	864:866	arg1	%					869:869	ADM 2%	864:869	ADM 2%	864:869	ADM 2% was administered by aerosol 1 and 6 h after LPS instillation.
36139146	1	60	theme	specific	205:212	arg1	therapy					214:220	no specific therapy	202:220	no specific therapy	202:220	Acute lung injury (ALI) is a common and devastating clinical disorder with a high mortality rate and no specific therapy.
36139146	4	61	from	effects	733:739	arg1	model					758:762	a mice model	751:762	a mice model	751:762	The aim of this study is to evaluate the protective effects of ADM in a mice model of ALI, induced by intratracheal administration of lipopolysaccharide (LPS) at the dose of 5 mg/kg.
36139146	2	62	theme	structural	353:362	arg1	damage					364:369	structural damage	353:369	structural damage	353:369	The pathophysiology of ALI is characterized by increased alveolar/capillary permeability, lung inflammation, oxidative stress and structural damage to lung tissues, which can progress to acute respiratory distress syndrome (ARDS).
36139146	7	63	theme	MC	1105:1106	arg1	number					1108:1113	MC number	1105:1113	MC number	1105:1113	At the same time, ADM counteracted the increase in MC number and the expression of specific markers of MC activation, i.e., chymase and tryptase.
37033116	4	0	theme	weight	488:493	arg1	percent					495:501	the weight percent	484:501	the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7	484:538	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	4	1	theme	sugar	371:375	arg1	composition					377:387	Main sugar composition	366:387	Main sugar composition	366:387	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	4	2	from	Man	450:452	arg1	percent					495:501	the weight percent	484:501	the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7	484:538	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	4	3	theme	21.7	535:538	arg1	percent					495:501	the weight percent	484:501	the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7	484:538	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	6	4	theme	native	853:858	arg1	SP					860:861	native SP	853:861	native SP	853:861	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	5	5	theme	trifluoroacetic	603:617	arg1	TFA					625:627	TFA	625:627	TFA	625:627	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	5	5	theme	trifluoroacetic	603:617	arg1	acid					619:622	trifluoroacetic acid	603:622	trifluoroacetic acid (TFA)	603:628	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	4	6	theme	Gal	435:437	arg1	Man					450:452	Gal, Glc, Xyl, Man	435:452	Man	450:452	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	3	7	with	SP	278:279	arg1	IC50					286:289	IC50	286:289	IC50 of 163 µg/mL	286:302	Results showed that SP with IC50 of 163 µg/mL appears to allosterically inhibit the hyaluronidase activity.
37033116	6	8	theme	hyaluronidase	932:944	arg1	inhibition					946:955	hyaluronidase inhibition	932:955	hyaluronidase inhibition	932:955	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	5	9	theme	content	667:673	arg1	effect					649:654	the effect	645:654	the effect of sulfate content and molecular weight on inhibition	645:708	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	6	10	theme	0.1	763:765	arg1	M					767:767	M	767:767	M	767:767	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	2	11	theme	sulfated	201:208	arg1	SP					226:227	SP	226:227	SP	226:227	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	2	11	theme	sulfated	201:208	arg1	polysaccharide					210:223	sulfated polysaccharide	201:223	sulfated polysaccharide (SP) from Caulerpa lentillifera	201:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	5	12	from	effect	649:654	arg1	inhibition					699:708	inhibition	699:708	inhibition	699:708	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	5	13	theme	molecular	679:687	arg1	weight					689:694	molecular weight	679:694	molecular weight	679:694	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	6	14	theme	SP	759:760	arg1	activity					736:743	Hyaluronidase inhibitory activity	711:743	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP	711:813	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	2	15	from	lentillifera	244:255	arg1	SP					226:227	SP	226:227	SP	226:227	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	2	15	from	lentillifera	244:255	arg1	activity					189:196	hyaluronidase inhibitory activity	164:196	hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera	164:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	2	15	from	lentillifera	244:255	arg1	polysaccharide					210:223	sulfated polysaccharide	201:223	sulfated polysaccharide (SP) from Caulerpa lentillifera	201:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	3	16	theme	163	294:296	arg1	µg/mL					298:302	163 µg/mL	294:302	163 µg/mL	294:302	Results showed that SP with IC50 of 163 µg/mL appears to allosterically inhibit the hyaluronidase activity.
37033116	6	17	theme	desulfated	748:757	arg1	SP					759:760	desulfated SP	748:760	desulfated SP	748:760	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	2	18	theme	inhibitory	178:187	arg1	activity					189:196	hyaluronidase inhibitory activity	164:196	hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera	164:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	4	19	from	sulfate	473:479	arg1	percent					495:501	the weight percent	484:501	the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7	484:538	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	4	20	theme	SP	412:413	arg1	composition					377:387	Main sugar composition	366:387	Main sugar composition	366:387	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	4	20	theme	SP	412:413	arg1	content					401:407	sulfate content	393:407	sulfate content of SP	393:413	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	6	21	theme	M	795:795	arg1	SP					812:813	0.5 M TFA-hydrolyzed SP	791:813	0.5 M TFA-hydrolyzed SP	791:813	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	1	22	theme	effective	115:123	arg1	inhibitors					139:148	effective hyaluronidase inhibitors	115:148	effective hyaluronidase inhibitors	115:148	Algal sulfated polysaccharides are known to be effective hyaluronidase inhibitors.
37033116	0	23	theme	Caulerpa	45:52	arg1	lentillifera					54:65	Caulerpa lentillifera	45:65	Caulerpa lentillifera	45:65	Hyaluronidase-inhibiting Polysaccharide from Caulerpa lentillifera.
37033116	6	24	theme	inhibitory	725:734	arg1	activity					736:743	Hyaluronidase inhibitory activity	711:743	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP	711:813	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	1	25	theme	hyaluronidase	125:137	arg1	inhibitors					139:148	effective hyaluronidase inhibitors	115:148	effective hyaluronidase inhibitors	115:148	Algal sulfated polysaccharides are known to be effective hyaluronidase inhibitors.
37033116	6	26	theme	M	767:767	arg1	SP					784:785	0.1 M TFA-hydrolyzed SP	763:785	0.1 M TFA-hydrolyzed SP	763:785	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	6	27	theme	molecular	898:906	arg1	weight					908:913	molecular weight	898:913	molecular weight	898:913	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	4	28	from	acids	462:466	arg1	percent					495:501	the weight percent	484:501	the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7	484:538	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	4	29	theme	uronic	455:460	arg1	acids					462:466	uronic acids	455:466	uronic acids	455:466	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	2	30	from	activity	189:196	arg1	lentillifera					244:255	Caulerpa lentillifera	235:255	Caulerpa lentillifera	235:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	5	31	theme	partial	579:585	arg1	hydrolysis					587:596	partial hydrolysis	579:596	partial hydrolysis	579:596	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	3	32	theme	µg/mL	298:302	arg1	IC50					286:289	IC50	286:289	IC50 of 163 µg/mL	286:302	Results showed that SP with IC50 of 163 µg/mL appears to allosterically inhibit the hyaluronidase activity.
37033116	6	33	theme	SP	812:813	arg1	activity					736:743	Hyaluronidase inhibitory activity	711:743	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP	711:813	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	5	34	theme	sulfate	659:665	arg1	content					667:673	sulfate content	659:673	sulfate content	659:673	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	6	35	theme	TFA-hydrolyzed	797:810	arg1	SP					812:813	0.5 M TFA-hydrolyzed SP	791:813	0.5 M TFA-hydrolyzed SP	791:813	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	4	36	theme	sulfate	393:399	arg1	content					401:407	sulfate content	393:407	sulfate content of SP	393:413	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	5	37	theme	weight	689:694	arg1	effect					649:654	the effect	645:654	the effect of sulfate content and molecular weight on inhibition	645:708	We modified the SP by desulfation and partial hydrolysis with trifluoroacetic acid (TFA) to investigate the effect of sulfate content and molecular weight on inhibition.
37033116	6	38	theme	sulfate	879:885	arg1	content					887:893	sulfate content	879:893	sulfate content	879:893	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	3	39	theme	hyaluronidase	342:354	arg1	activity					356:363	the hyaluronidase activity	338:363	the hyaluronidase activity	338:363	Results showed that SP with IC50 of 163 µg/mL appears to allosterically inhibit the hyaluronidase activity.
37033116	2	40	theme	polysaccharide	210:223	arg1	activity					189:196	hyaluronidase inhibitory activity	164:196	hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera	164:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	6	41	theme	0.5	791:793	arg1	M					795:795	M	795:795	M	795:795	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	0	42	from	lentillifera	54:65	arg1	Polysaccharide					25:38	Polysaccharide	25:38	Polysaccharide	25:38	Hyaluronidase-inhibiting Polysaccharide from Caulerpa lentillifera.
37033116	4	43	dep	Man	450:452	arg1	Xyl					445:447	Gal, Glc, Xyl, Man	435:452	Xyl	445:447	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	4	43	dep	Man	450:452	arg1	Glc					440:442	Gal, Glc, Xyl, Man	435:452	Glc	440:442	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	2	44	theme	Caulerpa	235:242	arg1	lentillifera					244:255	Caulerpa lentillifera	235:255	Caulerpa lentillifera	235:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	6	45	theme	Hyaluronidase	711:723	arg1	activity					736:743	Hyaluronidase inhibitory activity	711:743	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP	711:813	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	4	46	theme	Main	366:369	arg1	composition					377:387	Main sugar composition	366:387	Main sugar composition	366:387	Main sugar composition and sulfate content of SP was estimated to be Gal, Glc, Xyl, Man, uronic acids, and sulfate in the weight percent of 27.7: 28.9: 14.6: 22.5: 3.4: 21.7.
37033116	6	47	theme	TFA-hydrolyzed	769:782	arg1	SP					784:785	0.1 M TFA-hydrolyzed SP	763:785	0.1 M TFA-hydrolyzed SP	763:785	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	1	48	theme	Algal	68:72	arg1	polysaccharides					83:97	Algal sulfated polysaccharides	68:97	Algal sulfated polysaccharides	68:97	Algal sulfated polysaccharides are known to be effective hyaluronidase inhibitors.
37033116	6	49	theme	SP	784:785	arg1	activity					736:743	Hyaluronidase inhibitory activity	711:743	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP	711:813	Hyaluronidase inhibitory activity of desulfated SP, 0.1 M TFA-hydrolyzed SP and 0.5 M TFA-hydrolyzed SP were significantly lower than that of native SP, revealing that sulfate content or molecular weight is important for hyaluronidase inhibition.
37033116	2	50	theme	hyaluronidase	164:176	arg1	activity					189:196	hyaluronidase inhibitory activity	164:196	hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera	164:255	We evaluated hyaluronidase inhibitory activity of sulfated polysaccharide (SP) from Caulerpa lentillifera.
37033116	1	51	theme	sulfated	74:81	arg1	polysaccharides					83:97	Algal sulfated polysaccharides	68:97	Algal sulfated polysaccharides	68:97	Algal sulfated polysaccharides are known to be effective hyaluronidase inhibitors.
37256230	5	0	theme	induced	752:758	arg1	mice					778:781	cyclophosphamide induced immunosuppressive mice	735:781	cyclophosphamide induced immunosuppressive mice	735:781	The immunoregulatory function was further investigated in cyclophosphamide induced immunosuppressive mice, murine splenocytes and macrophages.
37256230	6	1	theme	novel	831:835	arg1	polysaccharide					849:862	A novel homogeneous polysaccharide	829:862	A novel homogeneous polysaccharide (PNPB1)	829:870	Results: A novel homogeneous polysaccharide (PNPB1) was isolated from notoginseng with the molecular weight of 9.3 × 105 Da.
37256230	6	1	theme	novel	831:835	arg1	PNPB1					865:869	PNPB1	865:869	PNPB1	865:869	Results: A novel homogeneous polysaccharide (PNPB1) was isolated from notoginseng with the molecular weight of 9.3 × 105 Da.
37256230	8	2	theme	white	1295:1299	arg1	WBC					1314:1316	WBC	1314:1316	WBC	1314:1316	The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC).
37256230	8	2	theme	white	1295:1299	arg1	cells					1307:1311	the white blood cells	1291:1311	the white blood cells (WBC)	1291:1317	The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC).
37256230	0	3	theme	Panax	89:93	arg1	notoginseng					95:105	Panax notoginseng	89:105	Panax notoginseng	89:105	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	0	4	from	characterization	11:26	arg1	notoginseng					95:105	Panax notoginseng	89:105	Panax notoginseng	89:105	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	5	5	theme	immunosuppressive	760:776	arg1	mice					778:781	cyclophosphamide induced immunosuppressive mice	735:781	cyclophosphamide induced immunosuppressive mice	735:781	The immunoregulatory function was further investigated in cyclophosphamide induced immunosuppressive mice, murine splenocytes and macrophages.
37256230	10	6	theme	notoginseng	1669:1679	arg1	polysaccharides					1681:1695	notoginseng polysaccharides	1669:1695	notoginseng polysaccharides	1669:1695	Discussion: These findings provide a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides.
37256230	4	7	dep	transform	540:548	arg1	infrared					550:557	infrared	550:557	transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy	540:674	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	3	8	attach	isolated	406:413	arg1	notoginseng					420:430	notoginseng	420:430	notoginseng	420:430	Methods: The polysaccharide was isolated from notoginseng by anion exchange and gel permeation chromatography.
37256230	3	8	attach	isolated	406:413	arg2	polysaccharide					387:400	The polysaccharide	383:400	The polysaccharide	383:400	Methods: The polysaccharide was isolated from notoginseng by anion exchange and gel permeation chromatography.
37256230	3	9	theme	gel	454:456	arg1	chromatography					469:482	gel permeation chromatography	454:482	gel permeation chromatography	454:482	Methods: The polysaccharide was isolated from notoginseng by anion exchange and gel permeation chromatography.
37256230	2	10	theme	potential	337:345	arg1	activity					364:371	potential immunomodulatory activity	337:371	potential immunomodulatory activity	337:371	This study aimed to isolate polysaccharides from notoginseng and investigate the structural feature and potential immunomodulatory activity.
37256230	4	11	dep	Fourier	532:538	arg1	transform					540:548	transform	540:548	transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy	540:674	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	4	12	theme	FT-IR	560:564	arg1	spectroscopy					567:578	(FT-IR) spectroscopy	559:578	(FT-IR) spectroscopy	559:578	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	9	13	theme	TLR2	1474:1477	arg1	expression					1479:1488	TLR2 expression	1474:1488	TLR2 expression	1474:1488	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	7	14	dep	α-Glcp	1107:1112	arg1	1→4					1095:1097	1→4	1095:1097	1→4	1095:1097	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	8	15	theme	blood	1301:1305	arg1	WBC					1314:1316	WBC	1314:1316	WBC	1314:1316	The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC).
37256230	8	15	theme	blood	1301:1305	arg1	cells					1307:1311	the white blood cells	1291:1311	the white blood cells (WBC)	1291:1317	The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC).
37256230	9	16	theme	cytokine	1390:1397	arg1	IL-10					1413:1417	NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production	1383:1439	IL-10	1413:1417	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	7	17	theme	-linked	1148:1154	arg1	β-Glcp					1156:1161	(1, 4→6)-linked β-Glcp	1140:1161	β-Glcp	1156:1161	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	7	18	theme	α-Glcp	1107:1112	arg1	backbone					1082:1089	the major backbone	1072:1089	the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp	1072:1161	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	6	19	theme	molecular	911:919	arg1	weight					921:926	the molecular weight	907:926	the molecular weight of 9.3 × 105 Da	907:942	Results: A novel homogeneous polysaccharide (PNPB1) was isolated from notoginseng with the molecular weight of 9.3 × 105 Da.
37256230	8	20	theme	body	1225:1228	arg1	weight					1230:1235	murine body weight	1218:1235	murine body weight	1218:1235	The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC).
37256230	7	21	theme	β-Glcp	1156:1161	arg1	backbone					1082:1089	the major backbone	1072:1089	the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp	1072:1161	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	2	22	theme	structural	314:323	arg1	feature					325:331	the structural feature	310:331	the structural feature	310:331	This study aimed to isolate polysaccharides from notoginseng and investigate the structural feature and potential immunomodulatory activity.
37256230	7	23	theme	Monosaccharide	945:958	arg1	analysis					972:979	Monosaccharide composition analysis	945:979	Monosaccharide composition analysis	945:979	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	7	24	link	-linked	1099:1105	arg1	α-Glcp					1107:1112	(1→4)-linked α-Glcp	1094:1112	(1→4)-linked α-Glcp	1094:1112	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	0	25	from	activity	49:56	arg1	notoginseng					95:105	Panax notoginseng	89:105	Panax notoginseng	89:105	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	9	26	theme	peritoneal	1493:1502	arg1	macrophages					1504:1514	peritoneal macrophages	1493:1514	peritoneal macrophages	1493:1514	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	7	27	theme	composition	960:970	arg1	analysis					972:979	Monosaccharide composition analysis	945:979	Monosaccharide composition analysis	945:979	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	1	28	theme	immunomodulatory	207:222	arg1	ability					224:230	its immunomodulatory ability	203:230	its immunomodulatory ability	203:230	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
37256230	0	29	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	4	30	theme	magnetic	638:645	arg1	NMR					658:660	NMR	658:660	NMR	658:660	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	4	30	theme	magnetic	638:645	arg1	resonance					647:655	nuclear magnetic resonance	630:655	nuclear magnetic resonance (NMR) spectroscopy	630:674	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	5	31	theme	cyclophosphamide	735:750	arg1	mice					778:781	cyclophosphamide induced immunosuppressive mice	735:781	cyclophosphamide induced immunosuppressive mice	735:781	The immunoregulatory function was further investigated in cyclophosphamide induced immunosuppressive mice, murine splenocytes and macrophages.
37256230	7	32	dep	β-Glcp	1128:1133	arg1	1→6					1116:1118	1→6	1116:1118	1→6	1116:1118	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	7	33	theme	-linked	1120:1126	arg1	β-Glcp					1128:1133	(1→6)-linked β-Glcp	1115:1133	(1→6)-linked β-Glcp	1115:1133	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	3	34	theme	anion	435:439	arg1	exchange					441:448	anion exchange	435:448	anion exchange	435:448	Methods: The polysaccharide was isolated from notoginseng by anion exchange and gel permeation chromatography.
37256230	4	35	theme	nuclear	630:636	arg1	NMR					658:660	NMR	658:660	NMR	658:660	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	4	35	theme	nuclear	630:636	arg1	resonance					647:655	nuclear magnetic resonance	630:655	nuclear magnetic resonance (NMR) spectroscopy	630:674	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	2	36	from	notoginseng	282:292	arg1	polysaccharides					261:275	polysaccharides	261:275	polysaccharides from notoginseng	261:292	This study aimed to isolate polysaccharides from notoginseng and investigate the structural feature and potential immunomodulatory activity.
37256230	9	37	theme	macrophages	1504:1514	arg1	expression					1479:1488	TLR2 expression	1474:1488	TLR2 expression	1474:1488	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	9	37	theme	macrophages	1504:1514	arg1	IL-10					1413:1417	NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production	1383:1439	IL-10	1413:1417	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	9	37	theme	macrophages	1504:1514	arg1	proliferation					1368:1380	splenic lymphocyte proliferation	1349:1380	splenic lymphocyte proliferation	1349:1380	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	9	37	theme	macrophages	1504:1514	arg1	production					1430:1439	NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production	1383:1439	production	1430:1439	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	9	37	theme	macrophages	1504:1514	arg1	phagocytosis					1457:1468	phagocytosis	1457:1468	phagocytosis	1457:1468	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	10	38	theme	theoretical	1598:1608	arg1	basis					1610:1614	a theoretical basis	1596:1614	a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides	1596:1695	Discussion: These findings provide a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides.
37256230	0	39	from	notoginseng	95:105	arg1	polysaccharide					69:82	a novel polysaccharide	61:82	a novel polysaccharide from Panax notoginseng	61:105	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	0	39	from	notoginseng	95:105	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	0	39	from	notoginseng	95:105	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	2	40	theme	immunomodulatory	347:362	arg1	activity					364:371	potential immunomodulatory activity	337:371	potential immunomodulatory activity	337:371	This study aimed to isolate polysaccharides from notoginseng and investigate the structural feature and potential immunomodulatory activity.
37256230	4	41	theme	resonance	647:655	arg1	spectroscopy					663:674	nuclear magnetic resonance (NMR) spectroscopy	630:674	nuclear magnetic resonance (NMR) spectroscopy	630:674	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	9	42	dep	phagocytosis	1457:1468	arg1	the					1453:1455	the	1453:1455	the	1453:1455	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	9	43	theme	potent	1528:1533	arg1	effect					1553:1558	potent immunoenhancement effect	1528:1558	potent immunoenhancement effect	1528:1558	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	8	44	theme	murine	1218:1223	arg1	weight					1230:1235	murine body weight	1218:1235	murine body weight	1218:1235	The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC).
37256230	7	45	theme	β-Glcp	1128:1133	arg1	backbone					1082:1089	the major backbone	1072:1089	the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp	1072:1161	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	9	46	theme	NO	1383:1384	arg1	IL-10					1413:1417	NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production	1383:1439	IL-10	1413:1417	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	0	47	theme	immunomodulatory	32:47	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	7	48	theme	trace	1055:1059	arg1	GlcA					1061:1064	trace GlcA	1055:1064	trace GlcA	1055:1064	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	7	49	link	-linked	1120:1126	arg1	β-Glcp					1128:1133	(1→6)-linked β-Glcp	1115:1133	(1→6)-linked β-Glcp	1115:1133	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	3	50	theme	permeation	458:467	arg1	chromatography					469:482	gel permeation chromatography	454:482	gel permeation chromatography	454:482	Methods: The polysaccharide was isolated from notoginseng by anion exchange and gel permeation chromatography.
37256230	5	51	theme	murine	784:789	arg1	splenocytes					791:801	murine splenocytes	784:801	murine splenocytes	784:801	The immunoregulatory function was further investigated in cyclophosphamide induced immunosuppressive mice, murine splenocytes and macrophages.
37256230	0	52	theme	polysaccharide	69:82	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	0	52	theme	polysaccharide	69:82	arg1	activity					49:56	immunomodulatory activity	32:56	immunomodulatory activity	32:56	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	10	53	dep	structure	1636:1644	arg1	the					1632:1634	the	1632:1634	the	1632:1634	Discussion: These findings provide a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides.
37256230	4	54	theme	chromatography-mass	585:603	arg1	GC-MS					619:623	GC-MS	619:623	GC-MS	619:623	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	4	54	theme	chromatography-mass	585:603	arg1	spectrometry					605:616	gas chromatography-mass spectrometry	581:616	gas chromatography-mass spectrometry (GC-MS)	581:624	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	7	55	theme	major	1076:1080	arg1	backbone					1082:1089	the major backbone	1072:1089	the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp	1072:1161	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	6	56	theme	Da	941:942	arg1	weight					921:926	the molecular weight	907:926	the molecular weight of 9.3 × 105 Da	907:942	Results: A novel homogeneous polysaccharide (PNPB1) was isolated from notoginseng with the molecular weight of 9.3 × 105 Da.
37256230	1	57	theme	important	142:150	arg1	components					152:161	important components	142:161	important components of Panax notoginseng that contribute to its immunomodulatory ability	142:230	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
37256230	1	57	theme	important	142:150	arg1	Introduction					108:119	Introduction	108:119	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.	108:231	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
37256230	1	57	theme	important	142:150	arg1	Polysaccharides					122:136	Polysaccharides	122:136	Polysaccharides	122:136	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
37256230	9	58	dep	IL-10	1413:1417	arg1	IL-2					1407:1410	NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production	1383:1439	IL-2	1407:1410	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	4	59	theme	preliminary	489:499	arg1	structure					501:509	Its preliminary structure	485:509	Its preliminary structure	485:509	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	5	60	theme	immunoregulatory	681:696	arg1	function					698:705	The immunoregulatory function	677:705	The immunoregulatory function	677:705	The immunoregulatory function was further investigated in cyclophosphamide induced immunosuppressive mice, murine splenocytes and macrophages.
37256230	10	61	theme	immune	1650:1655	arg1	activity					1657:1664	immune activity	1650:1664	immune activity	1650:1664	Discussion: These findings provide a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides.
37256230	10	62	theme	polysaccharides	1681:1695	arg1	activity					1657:1664	immune activity	1650:1664	immune activity	1650:1664	Discussion: These findings provide a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides.
37256230	10	62	theme	polysaccharides	1681:1695	arg1	structure					1636:1644	structure	1636:1644	structure	1636:1644	Discussion: These findings provide a theoretical basis for elucidating the structure and immune activity of notoginseng polysaccharides.
37256230	9	63	theme	splenic	1349:1355	arg1	proliferation					1368:1380	splenic lymphocyte proliferation	1349:1380	splenic lymphocyte proliferation	1349:1380	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	7	64	link	-linked	1148:1154	arg1	β-Glcp					1156:1161	(1, 4→6)-linked β-Glcp	1140:1161	β-Glcp	1156:1161	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	7	65	theme	-linked	1099:1105	arg1	α-Glcp					1107:1112	(1→4)-linked α-Glcp	1094:1112	(1→4)-linked α-Glcp	1094:1112	Monosaccharide composition analysis indicated that PNPB1 consisted of Glc (88.2%), Gal (9.0%), Ara (2.4%) and trace GlcA, with the major backbone of (1→4)-linked α-Glcp, (1→6)-linked β-Glcp, and (1, 4→6)-linked β-Glcp.
37256230	9	66	theme	IFN-γ	1423:1427	arg1	production					1430:1439	NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production	1383:1439	production	1430:1439	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	6	67	theme	×	935:935	arg1	Da					941:942	9.3 × 105 Da	931:942	9.3 × 105 Da	931:942	Results: A novel homogeneous polysaccharide (PNPB1) was isolated from notoginseng with the molecular weight of 9.3 × 105 Da.
37256230	9	68	theme	immunoenhancement	1535:1551	arg1	effect					1553:1558	potent immunoenhancement effect	1528:1558	potent immunoenhancement effect	1528:1558	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	1	69	theme	Panax	166:170	arg1	notoginseng					172:182	Panax notoginseng	166:182	Panax notoginseng	166:182	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
37256230	9	70	theme	TNF-α	1400:1404	arg1	IL-10					1413:1417	NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production	1383:1439	IL-10	1413:1417	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	9	71	theme	lymphocyte	1357:1366	arg1	proliferation					1368:1380	splenic lymphocyte proliferation	1349:1380	splenic lymphocyte proliferation	1349:1380	PNPB1 significantly enhanced splenic lymphocyte proliferation, NO and cytokine (TNF-α, IL-2, IL-10 and IFN-γ) production, as well as the phagocytosis and TLR2 expression of peritoneal macrophages, indicating potent immunoenhancement effect.
37256230	0	72	theme	novel	63:67	arg1	polysaccharide					69:82	a novel polysaccharide	61:82	a novel polysaccharide from Panax notoginseng	61:105	Structural characterization and immunomodulatory activity of a novel polysaccharide from Panax notoginseng.
37256230	4	73	theme	gas	581:583	arg1	GC-MS					619:623	GC-MS	619:623	GC-MS	619:623	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	4	73	theme	gas	581:583	arg1	spectrometry					605:616	gas chromatography-mass spectrometry	581:616	gas chromatography-mass spectrometry (GC-MS)	581:624	Its preliminary structure was characterized by Fourier transform infrared (FT-IR) spectroscopy, gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) spectroscopy.
37256230	8	74	dep	thymus	1252:1257	arg1	indices					1270:1276	indices	1270:1276	indices	1270:1276	The polysaccharide was found to significantly enhance murine body weight, improve their thymus and spleen indices and increase the white blood cells (WBC).
37256230	6	75	theme	homogeneous	837:847	arg1	polysaccharide					849:862	A novel homogeneous polysaccharide	829:862	A novel homogeneous polysaccharide (PNPB1)	829:870	Results: A novel homogeneous polysaccharide (PNPB1) was isolated from notoginseng with the molecular weight of 9.3 × 105 Da.
37256230	6	75	theme	homogeneous	837:847	arg1	PNPB1					865:869	PNPB1	865:869	PNPB1	865:869	Results: A novel homogeneous polysaccharide (PNPB1) was isolated from notoginseng with the molecular weight of 9.3 × 105 Da.
37256230	1	76	theme	notoginseng	172:182	arg1	components					152:161	important components	142:161	important components of Panax notoginseng that contribute to its immunomodulatory ability	142:230	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
37256230	1	76	theme	notoginseng	172:182	arg1	Introduction					108:119	Introduction	108:119	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.	108:231	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
37256230	1	76	theme	notoginseng	172:182	arg1	Polysaccharides					122:136	Polysaccharides	122:136	Polysaccharides	122:136	Introduction: Polysaccharides are important components of Panax notoginseng that contribute to its immunomodulatory ability.
36009766	0	0	theme	Biomass	81:87	arg1	Production					89:98	Biomass Production	81:98	Biomass Production	81:98	Expression of Exogenous GFP-CesA6 in Tobacco Enhances Cell Wall Biosynthesis and Biomass Production.
36009766	7	1	theme	secondary	1366:1374	arg1	CesAs					1386:1390	all secondary cell wall CesAs	1362:1390	all secondary cell wall CesAs	1362:1390	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	8	2	from	differences	1634:1644	arg1	pectin					1649:1654	pectin	1649:1654	pectin	1649:1654	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	8	2	from	differences	1634:1644	arg1	composition					1683:1693	hemicellulose glycosyl composition	1660:1693	hemicellulose glycosyl composition	1660:1693	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	1	3	theme	economic	171:178	arg1	targets					180:186	important economic targets	161:186	important economic targets for several biotechnological applications including bioenergy and biofuel production	161:271	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	9	4	theme	tobacco	1767:1773	arg1	plants					1775:1780	tobacco plants	1767:1780	tobacco plants	1767:1780	Our results highlight the potentiality to overexpress primary CesAs in tobacco plants to enhance cellulose synthesis and biomass production.
36009766	6	5	dep	polysaccharides	1171:1185	arg1	NtGal10					1227:1233	NtGal10	1227:1233	NtGal10	1227:1233	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	5	dep	polysaccharides	1171:1185	arg1	galacturonans					1204:1216	galacturonans	1204:1216	galacturonans	1204:1216	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	5	dep	polysaccharides	1171:1185	arg1	xyloglucans					1188:1198	xyloglucans	1188:1198	xyloglucans	1188:1198	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	6	theme	primary	1041:1047	arg1	NtCesA6					1078:1084	NtCesA6	1078:1084	NtCesA6	1078:1084	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	6	theme	primary	1041:1047	arg1	NtCesA1					1056:1062	NtCesA1	1056:1062	NtCesA1	1056:1062	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	6	theme	primary	1041:1047	arg1	CesAs					1049:1053	primary CesAs	1041:1053	primary CesAs (NtCesA1, NtCesA3, and NtCesA6)	1041:1085	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	6	theme	primary	1041:1047	arg1	NtCesA3					1065:1071	NtCesA3	1065:1071	NtCesA3	1065:1071	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	2	7	theme	complex	400:406	arg1	proteins					353:360	CesAs proteins	347:360	CesAs proteins	347:360	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	2	7	theme	complex	400:406	arg1	components					363:372	components	363:372	components of the cellulose synthase complex	363:406	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	8	8	theme	cellulose	1544:1552	arg1	polysaccharides					1565:1579	cellulose and matrix polysaccharides	1544:1579	cellulose and matrix polysaccharides	1544:1579	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	0	9	from	Expression	0:9	arg1	Tobacco					37:43	Tobacco	37:43	Tobacco	37:43	Expression of Exogenous GFP-CesA6 in Tobacco Enhances Cell Wall Biosynthesis and Biomass Production.
36009766	8	10	theme	polysaccharides	1565:1579	arg1	polysaccharides					1565:1579	cellulose and matrix polysaccharides	1544:1579	cellulose and matrix polysaccharides	1544:1579	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	8	10	theme	polysaccharides	1565:1579	arg1	amount					1534:1539	The amount	1530:1539	The amount of cellulose and matrix polysaccharides	1530:1579	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	6	11	dep	CesAs	1049:1053	arg1	NtCesA6					1078:1084	NtCesA6	1078:1084	NtCesA6	1078:1084	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	11	dep	CesAs	1049:1053	arg1	NtCesA1					1056:1062	NtCesA1	1056:1062	NtCesA1	1056:1062	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	11	dep	CesAs	1049:1053	arg1	CesAs					1049:1053	primary CesAs	1041:1053	primary CesAs (NtCesA1, NtCesA3, and NtCesA6)	1041:1085	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	11	dep	CesAs	1049:1053	arg1	NtCesA3					1065:1071	NtCesA3	1065:1071	NtCesA3	1065:1071	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	12	dep	the	994:996	arg1	leaves					998:1003	leaves	998:1003	leaves	998:1003	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	1	13	theme	cellulose	110:118	arg1	biosynthesis					120:131	Improved cellulose biosynthesis	101:131	Improved cellulose biosynthesis	101:131	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	8	14	theme	matrix	1558:1563	arg1	polysaccharides					1565:1579	cellulose and matrix polysaccharides	1544:1579	cellulose and matrix polysaccharides	1544:1579	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	5	15	theme	increased	908:916	arg1	flower					918:923	increased flower	908:923	increased flower	908:923	Furthermore, F31 line exhibited increased flower and seed number, and an advance of about 20 days in the anthesis.
36009766	3	16	theme	walls	538:542	arg1	composition					518:528	the chemical composition	505:528	the chemical composition of cell walls	505:542	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	2	17	theme	cellulose	319:327	arg1	biosynthesis					303:314	the biosynthesis	299:314	the biosynthesis of cellulose	299:327	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	9	18	theme	cellulose	1793:1801	arg1	synthesis					1803:1811	cellulose synthesis	1793:1811	cellulose synthesis	1793:1811	Our results highlight the potentiality to overexpress primary CesAs in tobacco plants to enhance cellulose synthesis and biomass production.
36009766	3	19	theme	data	493:496	arg1	Analyses					453:460	Analyses	453:460	Analyses of morphological and molecular data and of the chemical composition of cell walls	453:542	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	4	20	theme	secondary	821:829	arg1	xylem					831:835	lignified secondary xylem	811:835	lignified secondary xylem	811:835	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	1	21	theme	several	192:198	arg1	bioenergy					240:248	bioenergy	240:248	bioenergy	240:248	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	1	21	theme	several	192:198	arg1	production					262:271	biofuel production	254:271	biofuel production	254:271	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	1	21	theme	several	192:198	arg1	applications					217:228	several biotechnological applications	192:228	several biotechnological applications including bioenergy and biofuel production	192:271	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	6	22	theme	non-cellulosic	1156:1169	arg1	polysaccharides					1171:1185	non-cellulosic polysaccharides	1156:1185	non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10)	1156:1234	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	4	23	theme	growth	747:752	arg1	parameters					754:763	all evaluated growth parameters	733:763	all evaluated growth parameters	733:763	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	4	23	theme	growth	747:752	arg1	stem					774:777	stem	774:777	stem	774:777	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	4	23	theme	growth	747:752	arg1	length					788:793	root length	783:793	root length	783:793	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	7	24	theme	expression	1342:1351	arg1	level					1353:1357	the expression level	1338:1357	the expression level of all secondary cell wall CesAs	1338:1390	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	1	25	theme	biotechnological	200:215	arg1	bioenergy					240:248	bioenergy	240:248	bioenergy	240:248	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	1	25	theme	biotechnological	200:215	arg1	production					262:271	biofuel production	254:271	biofuel production	254:271	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	1	25	theme	biotechnological	200:215	arg1	applications					217:228	several biotechnological applications	192:228	several biotechnological applications including bioenergy and biofuel production	192:271	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	3	26	theme	apparent	674:681	arg1	aberrations					703:713	no apparent other morphological aberrations	671:713	no apparent other morphological aberrations	671:713	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	3	27	theme	Arabidopsis	605:615	arg1	CesA6					617:621	the Arabidopsis CesA6	601:621	the Arabidopsis CesA6 fused to GFP	601:634	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	9	28	theme	biomass	1817:1823	arg1	production					1825:1834	biomass production	1817:1834	biomass production	1817:1834	Our results highlight the potentiality to overexpress primary CesAs in tobacco plants to enhance cellulose synthesis and biomass production.
36009766	3	29	theme	molecular	483:491	arg1	data					493:496	morphological and molecular data	465:496	morphological and molecular data	465:496	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	0	30	theme	GFP-CesA6	24:32	arg1	Expression					0:9	Expression	0:9	Expression of Exogenous GFP-CesA6 in Tobacco	0:43	Expression of Exogenous GFP-CesA6 in Tobacco Enhances Cell Wall Biosynthesis and Biomass Production.
36009766	4	31	theme	evaluated	737:745	arg1	parameters					754:763	all evaluated growth parameters	733:763	all evaluated growth parameters	733:763	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	4	31	theme	evaluated	737:745	arg1	stem					774:777	stem	774:777	stem	774:777	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	4	31	theme	evaluated	737:745	arg1	length					788:793	root length	783:793	root length	783:793	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	6	32	theme	remodeling	1247:1256	arg1	biosynthesis					1140:1151	the biosynthesis	1136:1151	the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7)	1136:1319	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	1	33	theme	Improved	101:108	arg1	biosynthesis					120:131	Improved cellulose biosynthesis	101:131	Improved cellulose biosynthesis	101:131	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	3	34	theme	chemical	509:516	arg1	composition					518:528	the chemical composition	505:528	the chemical composition of cell walls	505:542	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	0	35	theme	Exogenous	14:22	arg1	GFP-CesA6					24:32	Exogenous GFP-CesA6	14:32	Exogenous GFP-CesA6	14:32	Expression of Exogenous GFP-CesA6 in Tobacco Enhances Cell Wall Biosynthesis and Biomass Production.
36009766	6	36	dep	remodeling	1247:1256	arg1	XTHs					1271:1274	XTHs	1271:1274	XTHs	1271:1274	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	36	dep	remodeling	1247:1256	arg1	NtExp11					1259:1265	NtExp11	1259:1265	NtExp11	1259:1265	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	37	theme	wall	1242:1245	arg1	remodeling					1247:1256	cell wall remodeling	1237:1256	cell wall remodeling (NtExp11 and XTHs)	1237:1275	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	2	38	theme	CesAs	347:351	arg1	proteins					353:360	CesAs proteins	347:360	CesAs proteins	347:360	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	2	38	theme	CesAs	347:351	arg1	components					363:372	components	363:372	components of the cellulose synthase complex	363:406	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	3	39	with	phenotype	656:664	arg1	aberrations					703:713	no apparent other morphological aberrations	671:713	no apparent other morphological aberrations	671:713	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	3	40	theme	composition	518:528	arg1	Analyses					453:460	Analyses	453:460	Analyses of morphological and molecular data and of the chemical composition of cell walls	453:542	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	4	41	theme	F31	723:725	arg1	line					727:730	the F31 line	719:730	the F31 line	719:730	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	6	42	theme	cell	1237:1240	arg1	remodeling					1247:1256	cell wall remodeling	1237:1256	cell wall remodeling (NtExp11 and XTHs)	1237:1275	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	7	43	dep	leaves	1331:1336	arg1	NtCesA7					1402:1408	NtCesA7	1402:1408	NtCesA7	1402:1408	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	7	43	dep	leaves	1331:1336	arg1	NtCesA8					1415:1421	NtCesA8	1415:1421	NtCesA8	1415:1421	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	7	43	dep	leaves	1331:1336	arg1	NtCesA4					1393:1399	NtCesA4	1393:1399	NtCesA4	1393:1399	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	6	44	theme	F31	1008:1010	arg1	seedlings					1012:1020	F31 seedlings	1008:1020	F31 seedlings	1008:1020	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	3	45	theme	morphological	465:477	arg1	data					493:496	morphological and molecular data	465:496	morphological and molecular data	465:496	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	3	46	theme	giant	649:653	arg1	phenotype					656:664	a "giant" phenotype	646:664	a "giant" phenotype with no apparent other morphological aberrations	646:713	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	2	47	dep	The	274:276	arg1	attempts					278:285	attempts	278:285	attempts	278:285	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	0	48	theme	Cell	54:57	arg1	Biosynthesis					64:75	Cell Wall Biosynthesis	54:75	Cell Wall Biosynthesis	54:75	Expression of Exogenous GFP-CesA6 in Tobacco Enhances Cell Wall Biosynthesis and Biomass Production.
36009766	7	49	theme	secondary	1471:1479	arg1	CesAs					1481:1485	both primary and secondary CesAs	1454:1485	both primary and secondary CesAs	1454:1485	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	3	50	theme	"	654:654	arg1	phenotype					656:664	a "giant" phenotype	646:664	a "giant" phenotype with no apparent other morphological aberrations	646:713	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	4	51	theme	lignified	811:819	arg1	xylem					831:835	lignified secondary xylem	811:835	lignified secondary xylem	811:835	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	2	52	theme	synthase	391:398	arg1	complex					400:406	the cellulose synthase complex	377:406	the cellulose synthase complex	377:406	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	6	53	theme	expansion	1287:1295	arg1	biosynthesis					1140:1151	the biosynthesis	1136:1151	the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7)	1136:1319	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	3	54	theme	other	683:687	arg1	aberrations					703:713	no apparent other morphological aberrations	671:713	no apparent other morphological aberrations	671:713	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	6	55	theme	polysaccharides	1171:1185	arg1	biosynthesis					1140:1151	the biosynthesis	1136:1151	the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7)	1136:1319	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	3	56	theme	tobacco	556:562	arg1	plants					564:569	tobacco plants	556:569	tobacco plants (F31 line)	556:580	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	3	56	theme	tobacco	556:562	arg1	line					576:579	F31 line	572:579	F31 line	572:579	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	2	57	theme	cellulose	381:389	arg1	complex					400:406	the cellulose synthase complex	377:406	the cellulose synthase complex	377:406	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	7	58	theme	primary	1459:1465	arg1	CesAs					1481:1485	both primary and secondary CesAs	1454:1485	both primary and secondary CesAs	1454:1485	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	3	59	theme	morphological	689:701	arg1	aberrations					703:713	no apparent other morphological aberrations	671:713	no apparent other morphological aberrations	671:713	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	7	60	theme	CesAs	1386:1390	arg1	level					1353:1357	the expression level	1338:1357	the expression level of all secondary cell wall CesAs	1338:1390	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	8	61	theme	glycosyl	1674:1681	arg1	composition					1683:1693	hemicellulose glycosyl composition	1660:1693	hemicellulose glycosyl composition	1660:1693	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	1	62	theme	plant	137:141	arg1	biomass					143:149	plant biomass	137:149	plant biomass	137:149	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	9	63	from	CesAs	1758:1762	arg1	plants					1775:1780	tobacco plants	1767:1780	tobacco plants	1767:1780	Our results highlight the potentiality to overexpress primary CesAs in tobacco plants to enhance cellulose synthesis and biomass production.
36009766	5	64	dep	an	946:947	arg1	advance					949:955	advance	949:955	advance	949:955	Furthermore, F31 line exhibited increased flower and seed number, and an advance of about 20 days in the anthesis.
36009766	7	65	theme	cell	1376:1379	arg1	CesAs					1386:1390	all secondary cell wall CesAs	1362:1390	all secondary cell wall CesAs	1362:1390	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36009766	4	66	theme	root	783:786	arg1	length					788:793	root length	783:793	root length	783:793	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	3	67	theme	cell	533:536	arg1	walls					538:542	cell walls	533:542	cell walls	533:542	Analyses of morphological and molecular data and of the chemical composition of cell walls showed that tobacco plants (F31 line), stably expressing the Arabidopsis CesA6 fused to GFP, exhibits a "giant" phenotype with no apparent other morphological aberrations.
36009766	9	68	theme	primary	1750:1756	arg1	CesAs					1758:1762	primary CesAs	1750:1762	primary CesAs in tobacco plants to enhance cellulose synthesis and biomass production	1750:1834	Our results highlight the potentiality to overexpress primary CesAs in tobacco plants to enhance cellulose synthesis and biomass production.
36009766	6	69	theme	cell	1282:1285	arg1	NtPIP1.1					1298:1305	NtPIP1.1	1298:1305	NtPIP1.1	1298:1305	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	69	theme	cell	1282:1285	arg1	expansion					1287:1295	cell expansion	1282:1295	cell expansion (NtPIP1.1 and NtPIP2.7)	1282:1319	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	69	theme	cell	1282:1285	arg1	NtPIP2.7					1311:1318	NtPIP2.7	1311:1318	NtPIP2.7	1311:1318	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	1	70	theme	biofuel	254:260	arg1	production					262:271	biofuel production	254:271	biofuel production	254:271	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	4	71	theme	leaf	796:799	arg1	size					801:804	leaf size	796:804	leaf size	796:804	In the F31 line, all evaluated growth parameters, such as stem and root length, leaf size, and lignified secondary xylem, were significantly higher than in wt.
36009766	6	72	dep	enhanced	1091:1098	arg1	as					1101:1102	as	1101:1102	as	1101:1102	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	6	73	theme	CesAs	1049:1053	arg1	expression					1027:1036	the expression	1023:1036	the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6)	1023:1085	In the leaves of F31 seedlings, the expression of primary CesAs (NtCesA1, NtCesA3, and NtCesA6) was enhanced, as well as of proteins involved in the biosynthesis of non-cellulosic polysaccharides (xyloglucans and galacturonans, NtXyl4, NtGal10), cell wall remodeling (NtExp11 and XTHs), and cell expansion (NtPIP1.1 and NtPIP2.7).
36009766	2	74	theme	consistent	433:442	arg1	results					444:450	consistent results	433:450	consistent results	433:450	The attempts to increase the biosynthesis of cellulose by overexpressing CesAs proteins, components of the cellulose synthase complex, has not always produced consistent results.
36009766	0	75	theme	Wall	59:62	arg1	Biosynthesis					64:75	Cell Wall Biosynthesis	54:75	Cell Wall Biosynthesis	54:75	Expression of Exogenous GFP-CesA6 in Tobacco Enhances Cell Wall Biosynthesis and Biomass Production.
36009766	8	76	theme	hemicellulose	1660:1672	arg1	composition					1683:1693	hemicellulose glycosyl composition	1660:1693	hemicellulose glycosyl composition	1660:1693	The amount of cellulose and matrix polysaccharides significantly increased in the F31 seedlings with no differences in pectin and hemicellulose glycosyl composition.
36009766	1	77	theme	important	161:169	arg1	targets					180:186	important economic targets	161:186	important economic targets for several biotechnological applications including bioenergy and biofuel production	161:271	Improved cellulose biosynthesis and plant biomass represent important economic targets for several biotechnological applications including bioenergy and biofuel production.
36009766	7	78	theme	wall	1381:1384	arg1	CesAs					1386:1390	all secondary cell wall CesAs	1362:1390	all secondary cell wall CesAs	1362:1390	While in leaves the expression level of all secondary cell wall CesAs (NtCesA4, NtCesA7, and NtCesA8) did not change significantly, both primary and secondary CesAs were differentially expressed in the stem.
36968038	0	0	theme	macroporous	73:83	arg1	resin					85:89	S-8 macroporous resin	69:89	S-8 macroporous resin	69:89	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	2	1	theme	macroporous	519:529	arg1	resin					542:546	S-8 macroporous adsorption resin	515:546	S-8 macroporous adsorption resin	515:546	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	4	2	theme	glucuronic	1175:1184	arg1	acid					1186:1189	glucuronic acid	1175:1189	glucuronic acid	1175:1189	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	3	3	theme	resin	824:828	arg1	adsorption					830:839	S-8 macroporous adsorption resin adsorption	797:839	S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS)	797:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	0	4	theme	S-8	69:71	arg1	resin					85:89	S-8 macroporous resin	69:89	S-8 macroporous resin	69:89	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	1	5	theme	antioxidant	358:368	arg1	activity					370:377	antioxidant activity	358:377	antioxidant activity	358:377	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	2	6	with	consistent	726:735	arg1	hypothesis					752:761	the model hypothesis	742:761	the model hypothesis	742:761	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	3	7	theme	alfalfa	865:871	arg1	polysaccharides					873:887	alfalfa polysaccharides	865:887	alfalfa polysaccharides (APS)	865:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	3	7	theme	alfalfa	865:871	arg1	APS					890:892	APS	890:892	APS	890:892	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	2	8	theme	adsorption	596:605	arg1	temperature					607:617	the adsorption temperature	592:617	the adsorption temperature	592:617	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	2	8	theme	adsorption	596:605	arg1	°C.					626:628	58 °C.	623:628	58 °C.	623:628	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	4	9	theme	monosaccharide	1073:1086	arg1	composition					1088:1098	monosaccharide composition	1073:1098	monosaccharide composition	1073:1098	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	3	10	theme	macroporous	801:811	arg1	adsorption					830:839	S-8 macroporous adsorption resin adsorption	797:839	S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS)	797:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	5	11	theme	hydroxyl	1356:1363	arg1	radicals					1365:1372	hydroxyl radicals	1356:1372	hydroxyl radicals	1356:1372	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	3	12	from	polysaccharides	873:887	arg1	adsorption					830:839	S-8 macroporous adsorption resin adsorption	797:839	S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS)	797:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	2	13	theme	model	746:750	arg1	hypothesis					752:761	the model hypothesis	742:761	the model hypothesis	742:761	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	3	14	theme	adsorption	813:822	arg1	adsorption					830:839	S-8 macroporous adsorption resin adsorption	797:839	S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS)	797:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	1	15	theme	decolorized	382:392	arg1	polysaccharides					394:408	decolorized polysaccharides	382:408	decolorized polysaccharides	382:408	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	2	16	theme	optimal	642:648	arg1	conditions					650:659	these optimal conditions	636:659	these optimal conditions	636:659	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	5	17	theme	strong	1306:1311	arg1	activity					1324:1331	strong scavenging activity	1306:1331	strong scavenging activity against ABTS, DPPH and hydroxyl radicals	1306:1372	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	2	18	theme	optimal	433:439	arg1	conditions					456:465	The optimal decolorization conditions	429:465	The optimal decolorization conditions	429:465	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	2	19	theme	S-8	515:517	arg1	resin					542:546	S-8 macroporous adsorption resin	515:546	S-8 macroporous adsorption resin	515:546	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	1	20	theme	polysaccharides	394:408	arg1	properties					343:352	the physicochemical properties	323:352	the physicochemical properties	323:352	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	1	20	theme	polysaccharides	394:408	arg1	activity					370:377	antioxidant activity	358:377	antioxidant activity	358:377	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	2	21	theme	71.43	688:692	arg1	±					694:694	±	694:694	±	694:694	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	3	22	theme	S-8	797:799	arg1	adsorption					830:839	S-8 macroporous adsorption resin adsorption	797:839	S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS)	797:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	5	23	theme	scavenging	1313:1322	arg1	activity					1324:1331	strong scavenging activity	1306:1331	strong scavenging activity against ABTS, DPPH and hydroxyl radicals	1306:1372	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	1	24	theme	decolorization	163:176	arg1	conditions					178:187	the decolorization conditions	159:187	the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin	159:265	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	0	25	theme	green	2:6	arg1	method					8:13	A green method	0:13	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.	0:142	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	0	26	from	decolorization	19:32	arg1	alfalfa					58:64	alfalfa	58:64	alfalfa	58:64	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	4	27	theme	chromatography	1039:1052	arg1	analysis					1061:1068	High-performance liquid chromatography (HPLC) analysis	1015:1068	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition	1015:1098	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	3	28	theme	pseudo-second-order	926:944	arg1	equations					946:954	the Freundlich and pseudo-second-order equations	907:954	the Freundlich and pseudo-second-order equations	907:954	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	1	29	theme	response	294:301	arg1	method					311:316	the response surface method	290:316	the response surface method	290:316	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	0	30	theme	antioxidant	122:132	arg1	activity					134:141	antioxidant activity	122:141	antioxidant activity	122:141	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	2	31	theme	decolorization	664:677	arg1	ratio					679:683	a decolorization ratio	662:683	a decolorization ratio of 71.43 ± 0.23%	662:700	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	3	32	from	molecules	852:860	arg1	polysaccharides					873:887	alfalfa polysaccharides	865:887	alfalfa polysaccharides (APS)	865:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	3	32	from	molecules	852:860	arg1	APS					890:892	APS	890:892	APS	890:892	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	1	33	theme	polysaccharides	192:206	arg1	conditions					178:187	the decolorization conditions	159:187	the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin	159:265	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	1	34	theme	surface	303:309	arg1	method					311:316	the response surface method	290:316	the response surface method	290:316	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	3	35	theme	Freundlich	911:920	arg1	equations					946:954	the Freundlich and pseudo-second-order equations	907:954	the Freundlich and pseudo-second-order equations	907:954	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	4	36	dep	ratio	1202:1206	arg1	 1.22 					1224:1229	 1.22 	1224:1229	 1.22 	1224:1229	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	4	36	dep	ratio	1202:1206	arg1	 8.04 					1217:1222	 8.04 	1217:1222	 8.04 	1217:1222	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	4	36	dep	ratio	1202:1206	arg1	 1					1238:1239	 1	1238:1239	 1	1238:1239	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	4	36	dep	ratio	1202:1206	arg1	 0.92 					1231:1236	 0.92 	1231:1236	 0.92 	1231:1236	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	5	37	theme	studies	1278:1284	arg1	results					1246:1252	The results	1242:1252	The results of antioxidant activity studies	1242:1284	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	2	38	theme	adsorption	563:572	arg1	time					574:577	the adsorption time	559:577	the adsorption time	559:577	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	2	38	theme	adsorption	563:572	arg1	h					585:585	2 h	583:585	2 h	583:585	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	4	39	theme	composition	1088:1098	arg1	analysis					1061:1068	High-performance liquid chromatography (HPLC) analysis	1015:1068	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition	1015:1098	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	5	40	theme	activity	1269:1276	arg1	studies					1278:1284	antioxidant activity studies	1257:1284	antioxidant activity studies	1257:1284	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	6	41	theme	pigment	1482:1488	arg1	molecules					1490:1498	polysaccharide pigment molecules	1467:1498	polysaccharide pigment molecules	1467:1498	This study will help to further understand the adsorption mechanism of macroporous resin on polysaccharide pigment molecules and lay a basis for evaluating their physiological activity.
36968038	4	42	theme	High-performance	1015:1030	arg1	HPLC					1055:1058	HPLC	1055:1058	HPLC	1055:1058	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	4	42	theme	High-performance	1015:1030	arg1	chromatography					1039:1052	High-performance liquid chromatography	1015:1052	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition	1015:1098	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	3	43	from	adsorption	830:839	arg1	polysaccharides					873:887	alfalfa polysaccharides	865:887	alfalfa polysaccharides (APS)	865:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	3	43	from	adsorption	830:839	arg1	APS					890:892	APS	890:892	APS	890:892	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	2	44	theme	decolorization	441:454	arg1	conditions					456:465	The optimal decolorization conditions	429:465	The optimal decolorization conditions	429:465	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	4	45	theme	liquid	1032:1037	arg1	HPLC					1055:1058	HPLC	1055:1058	HPLC	1055:1058	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	4	45	theme	liquid	1032:1037	arg1	chromatography					1039:1052	High-performance liquid chromatography	1015:1052	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition	1015:1098	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	3	46	theme	adsorption	768:777	arg1	curve					779:783	The adsorption curve	764:783	The adsorption curve	764:783	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	0	47	from	alfalfa	58:64	arg1	polysaccharides					37:51	polysaccharides	37:51	polysaccharides from alfalfa	37:64	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	0	47	from	alfalfa	58:64	arg1	decolorization					19:32	decolorization	19:32	decolorization of polysaccharides from alfalfa	19:64	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	2	48	theme	%	700:700	arg1	ratio					679:683	a decolorization ratio	662:683	a decolorization ratio of 71.43 ± 0.23%	662:700	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	0	49	theme	polysaccharides	37:51	arg1	decolorization					19:32	decolorization	19:32	decolorization of polysaccharides from alfalfa	19:64	A green method for decolorization of polysaccharides from alfalfa by S-8 macroporous resin and their characterization and antioxidant activity.
36968038	6	50	theme	resin	1458:1462	arg1	mechanism					1433:1441	the adsorption mechanism	1418:1441	the adsorption mechanism of macroporous resin on polysaccharide pigment molecules	1418:1498	This study will help to further understand the adsorption mechanism of macroporous resin on polysaccharide pigment molecules and lay a basis for evaluating their physiological activity.
36968038	2	51	theme	0.23	696:699	arg1	±					694:694	±	694:694	±	694:694	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	3	52	theme	molecules	852:860	arg1	adsorption					830:839	S-8 macroporous adsorption resin adsorption	797:839	S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS)	797:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	1	53	theme	physicochemical	327:341	arg1	properties					343:352	the physicochemical properties	323:352	the physicochemical properties	323:352	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	4	54	theme	1.18 	1211:1215	arg1	ratio					1202:1206	a molar ratio	1194:1206	a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1	1194:1239	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	6	55	from	mechanism	1433:1441	arg1	molecules					1490:1498	polysaccharide pigment molecules	1467:1498	polysaccharide pigment molecules	1467:1498	This study will help to further understand the adsorption mechanism of macroporous resin on polysaccharide pigment molecules and lay a basis for evaluating their physiological activity.
36968038	4	56	theme	molar	1196:1200	arg1	ratio					1202:1206	a molar ratio	1194:1206	a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1	1194:1239	High-performance liquid chromatography (HPLC) analysis of monosaccharide composition showed that APS was composed of mannose, glucose, galactose, arabinose and glucuronic acid in a molar ratio of 1.18 : 8.04 : 1.22 : 0.92 : 1.
36968038	6	57	theme	polysaccharide	1467:1480	arg1	molecules					1490:1498	polysaccharide pigment molecules	1467:1498	polysaccharide pigment molecules	1467:1498	This study will help to further understand the adsorption mechanism of macroporous resin on polysaccharide pigment molecules and lay a basis for evaluating their physiological activity.
36968038	5	58	contain	had	1302:1304	arg2	activity					1324:1331	strong scavenging activity	1306:1331	strong scavenging activity against ABTS, DPPH and hydroxyl radicals	1306:1372	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	5	58	contain	had	1302:1304	arg1	APS					1298:1300	APS	1298:1300	APS	1298:1300	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	6	59	theme	physiological	1537:1549	arg1	activity					1551:1558	their physiological activity	1531:1558	their physiological activity	1531:1558	This study will help to further understand the adsorption mechanism of macroporous resin on polysaccharide pigment molecules and lay a basis for evaluating their physiological activity.
36968038	3	60	theme	spontaneous	982:992	arg1	process					1006:1012	a spontaneous endothermic process	980:1012	a spontaneous endothermic process	980:1012	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	3	60	theme	spontaneous	982:992	arg1	adsorption					965:974	the adsorption	961:974	the adsorption	961:974	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	5	61	theme	antioxidant	1257:1267	arg1	studies					1278:1284	antioxidant activity studies	1257:1284	antioxidant activity studies	1257:1284	The results of antioxidant activity studies showed that APS had strong scavenging activity against ABTS, DPPH and hydroxyl radicals.
36968038	1	62	theme	S-8	234:236	arg1	resin					261:265	S-8 macroporous adsorption resin	234:265	S-8 macroporous adsorption resin	234:265	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	2	63	theme	resin	542:546	arg1	g					556:556	1.4 g	552:556	1.4 g	552:556	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	2	63	theme	resin	542:546	arg1	resin					542:546	S-8 macroporous adsorption resin	515:546	S-8 macroporous adsorption resin	515:546	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	2	63	theme	resin	542:546	arg1	amount					505:510	the amount	501:510	the amount of S-8 macroporous adsorption resin	501:546	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	6	64	theme	adsorption	1422:1431	arg1	mechanism					1433:1441	the adsorption mechanism	1418:1441	the adsorption mechanism of macroporous resin on polysaccharide pigment molecules	1418:1498	This study will help to further understand the adsorption mechanism of macroporous resin on polysaccharide pigment molecules and lay a basis for evaluating their physiological activity.
36968038	3	65	theme	endothermic	994:1004	arg1	process					1006:1012	a spontaneous endothermic process	980:1012	a spontaneous endothermic process	980:1012	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	3	65	theme	endothermic	994:1004	arg1	adsorption					965:974	the adsorption	961:974	the adsorption	961:974	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	3	66	theme	pigment	844:850	arg1	molecules					852:860	pigment molecules	844:860	pigment molecules in alfalfa polysaccharides (APS)	844:893	The adsorption curve showed that S-8 macroporous adsorption resin adsorption of pigment molecules in alfalfa polysaccharides (APS) agreed with the Freundlich and pseudo-second-order equations, and the adsorption was a spontaneous endothermic process.
36968038	1	67	theme	macroporous	238:248	arg1	resin					261:265	S-8 macroporous adsorption resin	234:265	S-8 macroporous adsorption resin	234:265	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
36968038	2	68	theme	adsorption	531:540	arg1	resin					542:546	S-8 macroporous adsorption resin	515:546	S-8 macroporous adsorption resin	515:546	The optimal decolorization conditions were determined to be as follows: the amount of S-8 macroporous adsorption resin was 1.4 g, the adsorption time was 2 h, and the adsorption temperature was 58 °C. Under these optimal conditions, a decolorization ratio of 71.43 ± 0.23% was achieved, which was consistent with the model hypothesis.
36968038	6	69	theme	macroporous	1446:1456	arg1	resin					1458:1462	macroporous resin	1446:1462	macroporous resin	1446:1462	This study will help to further understand the adsorption mechanism of macroporous resin on polysaccharide pigment molecules and lay a basis for evaluating their physiological activity.
36968038	1	70	theme	adsorption	250:259	arg1	resin					261:265	S-8 macroporous adsorption resin	234:265	S-8 macroporous adsorption resin	234:265	In this study, the decolorization conditions of polysaccharides extracted from alfalfa by S-8 macroporous adsorption resin were optimized through the response surface method, and the physicochemical properties and antioxidant activity of decolorized polysaccharides were investigated.
35450281	10	0	theme	halophilic	2143:2152	arg1	activity					2164:2171	halophilic bacterial activity	2143:2171	halophilic bacterial activity	2143:2171	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	1	1	theme	various	282:288	arg1	factors					304:310	the various hydrochemical factors	278:310	the various hydrochemical factors	278:310	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	7	2	theme	protodolomite	1369:1381	arg1	formation					1383:1391	protodolomite formation	1369:1391	protodolomite formation	1369:1391	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	10	3	theme	protodolomite	2121:2133	arg1	ratios					2093:2098	high Mg/Ca molar ratios	2076:2098	high Mg/Ca molar ratios	2076:2098	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	10	3	theme	protodolomite	2121:2133	arg1	formation					2108:2116	the formation	2104:2116	the formation of protodolomite through halophilic bacterial activity	2104:2171	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	3	4	theme	precipitates	685:696	arg1	structure					668:676	crystal lattice structure	652:676	crystal lattice structure	652:676	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	4	theme	precipitates	685:696	arg1	morphology					636:645	morphology	636:645	morphology	636:645	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	4	theme	precipitates	685:696	arg1	phases					605:610	The mineral phases	593:610	The mineral phases	593:610	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	4	theme	precipitates	685:696	arg1	composition					623:633	elemental composition	613:633	elemental composition	613:633	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	10	5	theme	present	2030:2036	arg1	findings					2038:2045	the present findings	2026:2045	the present findings	2026:2045	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	6	6	theme	Mg/Ca	1215:1219	arg1	ratios					1227:1232	relatively high Mg/Ca molar ratios	1199:1232	relatively high Mg/Ca molar ratios	1199:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	5	7	from	EPS	915:917	arg1	polysaccharides					892:906	polysaccharides	892:906	polysaccharides	892:906	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	5	7	from	EPS	915:917	arg1	amounts					865:871	The amounts	861:871	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios	861:964	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	5	7	from	EPS	915:917	arg1	acids					882:886	amino acids	876:886	amino acids	876:886	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	1	8	theme	protodolomite	185:197	arg1	precipitation					199:211	protodolomite precipitation	185:211	protodolomite precipitation	185:211	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	2	9	theme	200‰	587:590	arg1	salinity					575:582	a salinity	573:582	a salinity of 200‰	573:590	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	2	9	theme	200‰	587:590	arg1	ratios					540:545	various Mg/Ca molar ratios	520:545	various Mg/Ca molar ratios (0, 3, 6, 10, and 12)	520:567	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	9	10	theme	total	1810:1814	arg1	amount					1820:1825	the total EPS amount	1806:1825	the total EPS amount	1806:1825	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	6	11	theme	disordered	1077:1086	arg1	protodolomite					1103:1115	disordered stoichiometric protodolomite	1077:1115	disordered stoichiometric protodolomite	1077:1115	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	10	12	theme	previous	2008:2015	arg1	studies					2017:2023	previous studies	2008:2023	previous studies	2008:2023	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	8	13	theme	Mg/Ca	1642:1646	arg1	ratio					1654:1658	the high Mg/Ca molar ratio	1633:1658	the high Mg/Ca molar ratio	1633:1658	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35450281	1	14	theme	aqueous	225:231	arg1	conditions					233:242	specific aqueous conditions	216:242	specific aqueous conditions	216:242	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	5	15	theme	acid	994:997	arg1	analyzer					999:1006	an amino acid analyzer	985:1006	an amino acid analyzer	985:1006	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	9	16	theme	other	1837:1841	arg1	facilitators					1843:1854	other facilitators	1837:1854	other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS	1837:1932	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	16	theme	other	1837:1841	arg1	polysaccharides					1907:1921	polysaccharides	1907:1921	polysaccharides	1907:1921	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	16	theme	other	1837:1841	arg1	acids					1883:1887	the acidic amino acids	1866:1887	the acidic amino acids (Glu and Asp)	1866:1901	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	4	17	from	weight	762:767	arg1	biominerals					798:808	the biominerals	794:808	the biominerals	794:808	The organic weight and functional groups in the biominerals were identified by TG-DSC, FTIR, and XPS analysis.
35450281	1	18	theme	Bacterial	125:133	arg1	activities					135:144	Bacterial activities	125:144	Bacterial activities	125:144	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	6	19	theme	bacteria	1173:1180	arg1	activities					1159:1168	the activities	1155:1168	the activities of bacteria in a medium with relatively high Mg/Ca molar ratios	1155:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	2	20	theme	molar	534:538	arg1	ratios					540:545	various Mg/Ca molar ratios	520:545	various Mg/Ca molar ratios (0, 3, 6, 10, and 12)	520:567	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	9	21	theme	acidic	1870:1875	arg1	Asp					1898:1900	Asp	1898:1900	Asp	1898:1900	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	21	theme	acidic	1870:1875	arg1	Glu					1890:1892	Glu	1890:1892	Glu	1890:1892	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	21	theme	acidic	1870:1875	arg1	acids					1883:1887	the acidic amino acids	1866:1887	the acidic amino acids (Glu and Asp)	1866:1901	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	6	22	from	medium	1187:1192	arg1	activities					1159:1168	the activities	1155:1168	the activities of bacteria in a medium with relatively high Mg/Ca molar ratios	1155:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	8	23	theme	organic	1691:1697	arg1	components					1699:1708	the organic components	1687:1708	the organic components	1687:1708	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35450281	6	24	with	medium	1187:1192	arg1	ratios					1227:1232	relatively high Mg/Ca molar ratios	1199:1232	relatively high Mg/Ca molar ratios	1199:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	2	25	theme	various	520:526	arg1	ratios					540:545	various Mg/Ca molar ratios	520:545	various Mg/Ca molar ratios (0, 3, 6, 10, and 12)	520:567	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	0	26	theme	Extreme	75:81	arg1	QPL2					119:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	2	27	from	mud	488:490	arg1	bacterium					466:474	a newly isolated extreme halophilic bacterium	430:474	a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2,	430:512	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	10	28	theme	Mg/Ca	2081:2085	arg1	ratios					2093:2098	high Mg/Ca molar ratios	2076:2098	high Mg/Ca molar ratios	2076:2098	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	0	29	theme	Bacterium	94:102	arg1	QPL2					119:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	8	30	theme	bacterial	1717:1725	arg1	EPS					1727:1729	the bacterial EPS	1713:1729	the bacterial EPS	1713:1729	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35450281	5	31	theme	polysaccharides	892:906	arg1	polysaccharides					892:906	polysaccharides	892:906	polysaccharides	892:906	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	5	31	theme	polysaccharides	892:906	arg1	amounts					865:871	The amounts	861:871	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios	861:964	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	5	31	theme	polysaccharides	892:906	arg1	acids					882:886	amino acids	876:886	amino acids	876:886	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	7	32	theme	precipitated	1457:1468	arg1	coating					1531:1537	a mineral coating	1521:1537	a mineral coating around cells	1521:1550	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	7	32	theme	precipitated	1457:1468	arg1	crystals					1481:1488	the precipitated spherulite crystals	1453:1488	the precipitated spherulite crystals	1453:1488	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	7	32	theme	precipitated	1457:1468	arg1	content					1577:1583	high organic matter content	1557:1583	high organic matter content within the crystals	1557:1603	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	7	32	theme	precipitated	1457:1468	arg1	structures					1509:1518	pinhole structures	1501:1518	pinhole structures	1501:1518	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	0	33	theme	harveyi	111:117	arg1	QPL2					119:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	5	34	theme	liquid	1029:1034	arg1	chromatography					1036:1049	high-performance liquid chromatography	1012:1049	high-performance liquid chromatography	1012:1049	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	0	35	theme	High	0:3	arg1	Ratios					17:22	High Mg/Ca Molar Ratios	0:22	High Mg/Ca Molar Ratios	0:22	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	7	36	theme	matter	1570:1575	arg1	content					1577:1583	high organic matter content	1557:1583	high organic matter content within the crystals	1557:1603	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	2	37	theme	salina	481:486	arg1	mud					488:490	salina mud	481:490	salina mud	481:490	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	2	37	theme	salina	481:486	arg1	QPL2					508:511	Vibrio harveyi QPL2	493:511	Vibrio harveyi QPL2	493:511	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	0	38	theme	Molar	11:15	arg1	Ratios					17:22	High Mg/Ca Molar Ratios	0:22	High Mg/Ca Molar Ratios	0:22	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	2	39	dep	ratios	540:545	arg1	10					557:558	10	557:558	10	557:558	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	2	39	dep	ratios	540:545	arg1	12					565:566	12	565:566	12	565:566	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	5	40	theme	acids	882:886	arg1	polysaccharides					892:906	polysaccharides	892:906	polysaccharides	892:906	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	5	40	theme	acids	882:886	arg1	amounts					865:871	The amounts	861:871	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios	861:964	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	5	40	theme	acids	882:886	arg1	acids					882:886	amino acids	876:886	amino acids	876:886	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	7	41	theme	high	1557:1560	arg1	content					1577:1583	high organic matter content	1557:1583	high organic matter content within the crystals	1557:1603	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	3	42	theme	elemental	613:621	arg1	phases					605:610	The mineral phases	593:610	The mineral phases	593:610	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	42	theme	elemental	613:621	arg1	composition					623:633	elemental composition	613:633	elemental composition	613:633	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	2	43	theme	extreme	447:453	arg1	bacterium					466:474	a newly isolated extreme halophilic bacterium	430:474	a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2,	430:512	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	7	44	theme	bacterial	1421:1429	arg1	relicts					1431:1437	the significant bacterial relicts	1405:1437	the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals	1405:1603	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	5	45	theme	various	939:945	arg1	ratios					959:964	various Mg/Ca molar ratios	939:964	various Mg/Ca molar ratios	939:964	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	2	46	theme	harveyi	500:506	arg1	mud					488:490	salina mud	481:490	salina mud	481:490	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	2	46	theme	harveyi	500:506	arg1	QPL2					508:511	Vibrio harveyi QPL2	493:511	Vibrio harveyi QPL2	493:511	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	4	47	theme	functional	773:782	arg1	groups					784:789	functional groups	773:789	functional groups	773:789	The organic weight and functional groups in the biominerals were identified by TG-DSC, FTIR, and XPS analysis.
35450281	5	48	theme	molar	953:957	arg1	ratios					959:964	various Mg/Ca molar ratios	939:964	various Mg/Ca molar ratios	939:964	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	5	49	from	acids	882:886	arg1	EPS					915:917	the EPS	911:917	the EPS of QPL2	911:925	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	9	50	theme	high	1969:1972	arg1	ratios					1986:1991	the high Mg/Ca molar ratios	1965:1991	the high Mg/Ca molar ratios	1965:1991	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	51	theme	molar	1980:1984	arg1	ratios					1986:1991	the high Mg/Ca molar ratios	1965:1991	the high Mg/Ca molar ratios	1965:1991	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	2	52	theme	biomineralization	379:395	arg1	experiments					397:407	biomineralization experiments	379:407	biomineralization experiments	379:407	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	3	53	theme	crystal	652:658	arg1	structure					668:676	crystal lattice structure	652:676	crystal lattice structure	652:676	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	53	theme	crystal	652:658	arg1	phases					605:610	The mineral phases	593:610	The mineral phases	593:610	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	5	54	from	polysaccharides	892:906	arg1	EPS					915:917	the EPS	911:917	the EPS of QPL2	911:925	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	1	55	theme	hydrochemical	290:302	arg1	factors					304:310	the various hydrochemical factors	278:310	the various hydrochemical factors	278:310	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	6	56	theme	molar	1221:1225	arg1	ratios					1227:1232	relatively high Mg/Ca molar ratios	1199:1232	relatively high Mg/Ca molar ratios	1199:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	7	57	theme	bacterial	1334:1342	arg1	activity					1344:1351	That bacterial activity	1329:1351	That bacterial activity	1329:1351	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	6	58	theme	molar	1301:1305	arg1	ratios					1307:1312	lower Mg/Ca molar ratios	1289:1312	lower Mg/Ca molar ratios (0, 3, and 6)	1289:1326	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	6	59	theme	high	1210:1213	arg1	ratios					1227:1232	relatively high Mg/Ca molar ratios	1199:1232	relatively high Mg/Ca molar ratios	1199:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	10	60	theme	bacterial	2154:2162	arg1	activity					2164:2171	halophilic bacterial activity	2143:2171	halophilic bacterial activity	2143:2171	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	6	61	theme	stoichiometric	1088:1101	arg1	protodolomite					1103:1115	disordered stoichiometric protodolomite	1077:1115	disordered stoichiometric protodolomite	1077:1115	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	9	62	theme	EPS	1816:1818	arg1	amount					1820:1825	the total EPS amount	1806:1825	the total EPS amount	1806:1825	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	63	dep	acids	1883:1887	arg1	Asp					1898:1900	Asp	1898:1900	Asp	1898:1900	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	63	dep	acids	1883:1887	arg1	Glu					1890:1892	Glu	1890:1892	Glu	1890:1892	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	63	dep	acids	1883:1887	arg1	acids					1883:1887	the acidic amino acids	1866:1887	the acidic amino acids (Glu and Asp)	1866:1901	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	1	64	theme	specific	216:223	arg1	conditions					233:242	specific aqueous conditions	216:242	specific aqueous conditions	216:242	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	4	65	theme	XPS	847:849	arg1	analysis					851:858	XPS analysis	847:858	XPS analysis	847:858	The organic weight and functional groups in the biominerals were identified by TG-DSC, FTIR, and XPS analysis.
35450281	5	66	theme	amino	988:992	arg1	analyzer					999:1006	an amino acid analyzer	985:1006	an amino acid analyzer	985:1006	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	8	67	theme	high	1637:1640	arg1	ratio					1654:1658	the high Mg/Ca molar ratio	1633:1658	the high Mg/Ca molar ratio	1633:1658	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35450281	4	68	dep	weight	762:767	arg1	The					750:752	The	750:752	The	750:752	The organic weight and functional groups in the biominerals were identified by TG-DSC, FTIR, and XPS analysis.
35450281	7	69	theme	mineral	1523:1529	arg1	coating					1531:1537	a mineral coating	1521:1537	a mineral coating around cells	1521:1550	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	6	70	dep	precipitated	1134:1145	arg1	12					1242:1243	12	1242:1243	12	1242:1243	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	6	70	dep	precipitated	1134:1145	arg1	10					1235:1236	10	1235:1236	10	1235:1236	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	6	71	theme	Mg/Ca	1295:1299	arg1	ratios					1307:1312	lower Mg/Ca molar ratios	1289:1312	lower Mg/Ca molar ratios (0, 3, and 6)	1289:1326	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	8	72	theme	molar	1648:1652	arg1	ratio					1654:1658	the high Mg/Ca molar ratio	1633:1658	the high Mg/Ca molar ratio	1633:1658	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35450281	9	73	from	amount	1820:1825	arg1	EPS					1930:1932	the EPS	1926:1932	the EPS	1926:1932	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	7	74	theme	pinhole	1501:1507	arg1	structures					1509:1518	pinhole structures	1501:1518	pinhole structures	1501:1518	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	6	75	with	cultures	1275:1282	arg1	ratios					1307:1312	lower Mg/Ca molar ratios	1289:1312	lower Mg/Ca molar ratios (0, 3, and 6)	1289:1326	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	2	76	theme	Mg/Ca	528:532	arg1	ratios					540:545	various Mg/Ca molar ratios	520:545	various Mg/Ca molar ratios (0, 3, 6, 10, and 12)	520:567	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	9	77	theme	amino	1877:1881	arg1	Asp					1898:1900	Asp	1898:1900	Asp	1898:1900	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	77	theme	amino	1877:1881	arg1	Glu					1890:1892	Glu	1890:1892	Glu	1890:1892	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	9	77	theme	amino	1877:1881	arg1	acids					1883:1887	the acidic amino acids	1866:1887	the acidic amino acids (Glu and Asp)	1866:1901	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	6	78	from	activities	1159:1168	arg1	medium					1187:1192	a medium	1185:1192	a medium with relatively high Mg/Ca molar ratios	1185:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	6	79	theme	lower	1289:1293	arg1	ratios					1307:1312	lower Mg/Ca molar ratios	1289:1312	lower Mg/Ca molar ratios (0, 3, and 6)	1289:1326	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	0	80	theme	Halophilic	83:92	arg1	QPL2					119:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	7	81	theme	spherulite	1470:1479	arg1	coating					1531:1537	a mineral coating	1521:1537	a mineral coating around cells	1521:1550	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	7	81	theme	spherulite	1470:1479	arg1	crystals					1481:1488	the precipitated spherulite crystals	1453:1488	the precipitated spherulite crystals	1453:1488	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	7	81	theme	spherulite	1470:1479	arg1	content					1577:1583	high organic matter content	1557:1583	high organic matter content within the crystals	1557:1603	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	7	81	theme	spherulite	1470:1479	arg1	structures					1509:1518	pinhole structures	1501:1518	pinhole structures	1501:1518	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	10	82	theme	molar	2087:2091	arg1	ratios					2093:2098	high Mg/Ca molar ratios	2076:2098	high Mg/Ca molar ratios	2076:2098	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	6	83	from	bacteria	1173:1180	arg1	medium					1187:1192	a medium	1185:1192	a medium with relatively high Mg/Ca molar ratios	1185:1232	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	0	84	theme	Vibrio	104:109	arg1	QPL2					119:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	the Extreme Halophilic Bacterium Vibrio harveyi QPL2	71:122	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	8	85	theme	components	1699:1708	arg1	composition					1672:1682	the composition	1668:1682	the composition of the organic components in the bacterial EPS	1668:1729	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35450281	10	86	theme	high	2076:2079	arg1	ratios					2093:2098	high Mg/Ca molar ratios	2076:2098	high Mg/Ca molar ratios	2076:2098	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	5	87	theme	high-performance	1012:1027	arg1	chromatography					1036:1049	high-performance liquid chromatography	1012:1049	high-performance liquid chromatography	1012:1049	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	6	88	dep	ratios	1307:1312	arg1	3					1318:1318	3	1318:1318	3	1318:1318	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	6	88	dep	ratios	1307:1312	arg1	6					1325:1325	6	1325:1325	6	1325:1325	The results confirm that disordered stoichiometric protodolomite was successfully precipitated through the activities of bacteria in a medium with relatively high Mg/Ca molar ratios (10 and 12) but it was not identified in cultures with lower Mg/Ca molar ratios (0, 3, and 6).
35450281	10	89	theme	clear	2057:2061	arg1	link					2063:2066	a clear link	2055:2066	a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity	2055:2171	Combined with previous studies, the present findings suggest a clear link between high Mg/Ca molar ratios and the formation of protodolomite through halophilic bacterial activity.
35450281	0	90	theme	Mg/Ca	5:9	arg1	Ratios					17:22	High Mg/Ca Molar Ratios	0:22	High Mg/Ca Molar Ratios	0:22	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	4	91	from	groups	784:789	arg1	biominerals					798:808	the biominerals	794:808	the biominerals	794:808	The organic weight and functional groups in the biominerals were identified by TG-DSC, FTIR, and XPS analysis.
35450281	1	92	theme	bacterial	316:324	arg1	activity					326:333	bacterial activity	316:333	bacterial activity	316:333	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	5	93	theme	amino	876:880	arg1	acids					882:886	amino acids	876:886	amino acids	876:886	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	9	94	from	facilitators	1843:1854	arg1	EPS					1930:1932	the EPS	1926:1932	the EPS	1926:1932	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	1	95	dep	demonstrated	156:167	arg1	whereas					245:251	whereas	245:251	whereas	245:251	Bacterial activities have been demonstrated as critical for protodolomite precipitation in specific aqueous conditions, whereas the relationship between the various hydrochemical factors and bacterial activity has not been fully explored.
35450281	2	96	theme	halophilic	455:464	arg1	bacterium					466:474	a newly isolated extreme halophilic bacterium	430:474	a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2,	430:512	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	0	97	theme	Protodolomite	32:44	arg1	Precipitation					46:58	Protodolomite Precipitation	32:58	Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2	32:122	High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2.
35450281	7	98	theme	organic	1562:1568	arg1	content					1577:1583	high organic matter content	1557:1583	high organic matter content within the crystals	1557:1603	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	3	99	theme	mineral	597:603	arg1	structure					668:676	crystal lattice structure	652:676	crystal lattice structure	652:676	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	99	theme	mineral	597:603	arg1	morphology					636:645	morphology	636:645	morphology	636:645	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	99	theme	mineral	597:603	arg1	phases					605:610	The mineral phases	593:610	The mineral phases	593:610	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	99	theme	mineral	597:603	arg1	composition					623:633	elemental composition	613:633	elemental composition	613:633	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	4	100	theme	organic	754:760	arg1	weight					762:767	organic weight	754:767	organic weight	754:767	The organic weight and functional groups in the biominerals were identified by TG-DSC, FTIR, and XPS analysis.
35450281	2	101	theme	isolated	438:445	arg1	bacterium					466:474	a newly isolated extreme halophilic bacterium	430:474	a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2,	430:512	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	5	102	theme	Mg/Ca	947:951	arg1	ratios					959:964	various Mg/Ca molar ratios	939:964	various Mg/Ca molar ratios	939:964	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	2	103	theme	Vibrio	493:498	arg1	mud					488:490	salina mud	481:490	salina mud	481:490	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	2	103	theme	Vibrio	493:498	arg1	QPL2					508:511	Vibrio harveyi QPL2	493:511	Vibrio harveyi QPL2	493:511	In this study, biomineralization experiments were conducted using a newly isolated extreme halophilic bacterium from salina mud, Vibrio harveyi QPL2, under various Mg/Ca molar ratios (0, 3, 6, 10, and 12) and a salinity of 200‰.
35450281	9	104	theme	Mg/Ca	1974:1978	arg1	ratios					1986:1991	the high Mg/Ca molar ratios	1965:1991	the high Mg/Ca molar ratios	1965:1991	Specifically, not only the total EPS amount, but also other facilitators including the acidic amino acids (Glu and Asp) and polysaccharides in the EPS, increased significantly under the high Mg/Ca molar ratios.
35450281	7	105	theme	significant	1409:1419	arg1	relicts					1431:1437	the significant bacterial relicts	1405:1437	the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals	1405:1603	That bacterial activity is critical for protodolomite formation as shown by the significant bacterial relicts identified in the precipitated spherulite crystals, including pinhole structures, a mineral coating around cells, and high organic matter content within the crystals.
35450281	5	106	from	amounts	865:871	arg1	EPS					915:917	the EPS	911:917	the EPS of QPL2	911:925	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	8	107	from	composition	1672:1682	arg1	EPS					1727:1729	the bacterial EPS	1713:1729	the bacterial EPS	1713:1729	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35450281	5	108	theme	QPL2	922:925	arg1	EPS					915:917	the EPS	911:917	the EPS of QPL2	911:925	The amounts of amino acids and polysaccharides in the EPS of QPL2 cultured at various Mg/Ca molar ratios were quantified by an amino acid analyzer and high-performance liquid chromatography.
35450281	3	109	theme	lattice	660:666	arg1	structure					668:676	crystal lattice structure	652:676	crystal lattice structure	652:676	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	3	109	theme	lattice	660:666	arg1	phases					605:610	The mineral phases	593:610	The mineral phases	593:610	The mineral phases, elemental composition, morphology, and crystal lattice structure of the precipitates were analyzed by XRD, SEM, and HRTEM, respectively.
35450281	8	110	theme	protodolomite	1768:1780	arg1	precipitation					1747:1759	the precipitation	1743:1759	the precipitation of the protodolomite	1743:1780	It was also confirmed that the high Mg/Ca molar ratio affects the composition of the organic components in the bacterial EPS, leading to the precipitation of the protodolomite.
35433782	2	0	theme	Hericium	561:568	arg1	erinaceus					570:578	Hericium erinaceus	561:578	Hericium erinaceus	561:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	5	1	dep	in	1015:1016	arg1	vitro					1018:1022	vitro	1018:1022	vitro	1018:1022	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	2	2	theme	GM	359:360	arg1	composition					362:372	GM composition	359:372	GM composition	359:372	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	4	3	theme	digestive	851:859	arg1	conditions					861:870	digestive conditions	851:870	digestive conditions simulating saliva, stomach, and small intestine	851:918	Under digestive conditions simulating saliva, stomach, and small intestine, HEPs were not significantly influenced and safely reached the distal intestine.
35433782	5	4	theme	sugars	1144:1149	arg1	rates					1116:1120	the retention rates	1102:1120	the retention rates of total and reducing sugars and pH value	1102:1162	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	2	5	from	effects	348:354	arg1	composition					362:372	GM composition	359:372	GM composition	359:372	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	3	6	from	production	809:818	arg1	fermentations					742:754	human fecal microbiota fermentations	719:754	human fecal microbiota fermentations	719:754	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	3	7	theme	fecal	725:729	arg1	fermentations					742:754	human fecal microbiota fermentations	719:754	human fecal microbiota fermentations	719:754	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	5	8	dep	decreased	1183:1191	arg1	<					1196:1196	p < 0.05	1194:1201	p < 0.05	1194:1201	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	3	9	from	effects	708:714	arg1	fermentations					742:754	human fecal microbiota fermentations	719:754	human fecal microbiota fermentations	719:754	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	0	10	theme	Female	123:128	arg1	Volunteers					130:139	Male and Female Volunteers	114:139	Male and Female Volunteers	114:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	5	11	theme	fermentation	1024:1035	arg1	h					1010:1010	24 h	1007:1010	24 h of in vitro fermentation	1007:1035	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	4	12	theme	small	904:908	arg1	intestine					910:918	small intestine	904:918	small intestine	904:918	Under digestive conditions simulating saliva, stomach, and small intestine, HEPs were not significantly influenced and safely reached the distal intestine.
35433782	3	13	theme	male	759:762	arg1	production					809:818	short-chain fatty acid (SCFA) production	779:818	short-chain fatty acid (SCFA) production in vitro	779:827	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	3	13	theme	male	759:762	arg1	effects					708:714	their effects	702:714	their effects on human fecal microbiota fermentations of male and female	702:773	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	1	14	theme	widespread	228:237	arg1	attention					239:247	widespread attention	228:247	widespread attention	228:247	Hericium erinaceus polysaccharides (HEPs) have attracted widespread attention in regulating gut microbiota (GM).
35433782	6	15	used	utilized	1225:1232	arg2	HEPs					1211:1214	HEPs	1211:1214	HEPs	1211:1214	Thus, HEPs could be utilized by GM, especially HEP-50, and enhanced the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus.
35433782	2	16	theme	hot	531:533	arg1	extracts					549:556	hot water-soluble extracts	531:556	hot water-soluble extracts of Hericium erinaceus	531:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	8	17	theme	extracted	1695:1703	arg1	foods					1753:1757	functional foods	1742:1757	functional foods with great development value	1742:1786	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	8	17	theme	extracted	1695:1703	arg1	polysaccharide					1705:1718	the extracted polysaccharide	1691:1718	the extracted polysaccharide	1691:1718	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	3	18	theme	short-chain	779:789	arg1	SCFA					803:806	SCFA	803:806	SCFA	803:806	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	3	18	theme	short-chain	779:789	arg1	acid					797:800	short-chain fatty acid	779:800	short-chain fatty acid (SCFA) production in vitro	779:827	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	5	19	theme	in	1015:1016	arg1	fermentation					1024:1035	in vitro fermentation	1015:1035	in vitro fermentation	1015:1035	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	2	20	from	digestibility	299:311	arg1	composition					362:372	GM composition	359:372	GM composition	359:372	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	3	21	theme	female	768:773	arg1	production					809:818	short-chain fatty acid (SCFA) production	779:818	short-chain fatty acid (SCFA) production in vitro	779:827	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	3	21	theme	female	768:773	arg1	effects					708:714	their effects	702:714	their effects on human fecal microbiota fermentations of male and female	702:773	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	0	22	theme	Positive	74:81	arg1	Effects					83:89	Their Positive Effects	68:89	Their Positive Effects on Fecal Microbiota of Male and Female Volunteers	68:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	2	23	from	fermentation	317:328	arg1	composition					362:372	GM composition	359:372	GM composition	359:372	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	5	24	theme	p	1087:1087	arg1	<					1089:1089	p < 0.05	1087:1094	p < 0.05	1087:1094	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	8	25	theme	functional	1742:1751	arg1	foods					1753:1757	functional foods	1742:1757	functional foods with great development value	1742:1786	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	8	25	theme	functional	1742:1751	arg1	polysaccharide					1705:1718	the extracted polysaccharide	1691:1718	the extracted polysaccharide	1691:1718	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	1	26	theme	Hericium	171:178	arg1	HEPs					207:210	HEPs	207:210	HEPs	207:210	Hericium erinaceus polysaccharides (HEPs) have attracted widespread attention in regulating gut microbiota (GM).
35433782	1	26	theme	Hericium	171:178	arg1	polysaccharides					190:204	Hericium erinaceus polysaccharides	171:204	Hericium erinaceus polysaccharides (HEPs)	171:211	Hericium erinaceus polysaccharides (HEPs) have attracted widespread attention in regulating gut microbiota (GM).
35433782	2	27	from	HEPs	333:336	arg1	composition					362:372	GM composition	359:372	GM composition	359:372	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	7	28	theme	pathogenic	1476:1485	arg1	bacteria					1487:1494	opportunistic pathogenic bacteria	1462:1494	opportunistic pathogenic bacteria	1462:1494	Furthermore, HEPs reduced the relative abundances of opportunistic pathogenic bacteria, e.g., Escherichia-Shigella, Klebsiella, and Enterobacter.
35433782	5	29	theme	p	1194:1194	arg1	<					1196:1196	p < 0.05	1194:1201	p < 0.05	1194:1201	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	3	30	theme	acid	797:800	arg1	production					809:818	short-chain fatty acid (SCFA) production	779:818	short-chain fatty acid (SCFA) production in vitro	779:827	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	7	31	dep	Escherichia-Shigella	1503:1522	arg1	e.g.					1497:1500	e.g.	1497:1500	e.g.	1497:1500	Furthermore, HEPs reduced the relative abundances of opportunistic pathogenic bacteria, e.g., Escherichia-Shigella, Klebsiella, and Enterobacter.
35433782	0	32	theme	Male	114:117	arg1	Volunteers					130:139	Male and Female Volunteers	114:139	Male and Female Volunteers	114:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	6	33	theme	bacteria	1314:1321	arg1	abundance					1286:1294	the relative abundance	1273:1294	the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus	1273:1406	Thus, HEPs could be utilized by GM, especially HEP-50, and enhanced the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus.
35433782	8	34	theme	development	1770:1780	arg1	value					1782:1786	great development value	1764:1786	great development value	1764:1786	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	0	35	theme	Digestive	0:8	arg1	Characteristics					10:24	Digestive Characteristics	0:24	Digestive Characteristics of Hericium erinaceus Polysaccharides	0:62	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	3	36	theme	prepared	610:617	arg1	HEPs					619:622	prepared HEPs	610:622	prepared HEPs	610:622	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	6	37	theme	SCFA-producing	1299:1312	arg1	bacteria					1314:1321	SCFA-producing bacteria	1299:1321	SCFA-producing bacteria	1299:1321	Thus, HEPs could be utilized by GM, especially HEP-50, and enhanced the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus.
35433782	0	38	theme	erinaceus	38:46	arg1	Polysaccharides					48:62	Hericium erinaceus Polysaccharides	29:62	Hericium erinaceus Polysaccharides	29:62	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	4	39	theme	distal	983:988	arg1	intestine					990:998	the distal intestine	979:998	the distal intestine	979:998	Under digestive conditions simulating saliva, stomach, and small intestine, HEPs were not significantly influenced and safely reached the distal intestine.
35433782	6	40	dep	Blautia	1365:1371	arg1	e.g.					1324:1327	e.g.	1324:1327	e.g.	1324:1327	Thus, HEPs could be utilized by GM, especially HEP-50, and enhanced the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus.
35433782	6	40	dep	Blautia	1365:1371	arg1	Bifidobacterium					1330:1344	Bifidobacterium	1330:1344	Bifidobacterium	1330:1344	Thus, HEPs could be utilized by GM, especially HEP-50, and enhanced the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus.
35433782	8	41	from	erinaceus	1676:1684	arg1	preparation					1631:1641	the preparation	1627:1641	the preparation of polysaccharides from Hericium erinaceus	1627:1684	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	8	41	from	erinaceus	1676:1684	arg1	polysaccharides					1646:1660	polysaccharides	1646:1660	polysaccharides from Hericium erinaceus	1646:1684	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	5	42	theme	value	1158:1162	arg1	rates					1116:1120	the retention rates	1102:1120	the retention rates of total and reducing sugars and pH value	1102:1162	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	8	43	theme	ethanol	1588:1594	arg1	precipitation					1596:1608	gradual ethanol precipitation	1580:1608	gradual ethanol precipitation	1580:1608	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	5	44	theme	total	1125:1129	arg1	sugars					1144:1149	total and reducing sugars	1125:1149	total and reducing sugars	1125:1149	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	3	45	theme	simulated	660:668	arg1	digestion					687:695	simulated gastrointestinal digestion	660:695	simulated gastrointestinal digestion	660:695	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	5	46	theme	reducing	1135:1142	arg1	sugars					1144:1149	total and reducing sugars	1125:1149	total and reducing sugars	1125:1149	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	2	47	theme	water-soluble	535:547	arg1	extracts					549:556	hot water-soluble extracts	531:556	hot water-soluble extracts of Hericium erinaceus	531:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	1	48	theme	erinaceus	180:188	arg1	HEPs					207:210	HEPs	207:210	HEPs	207:210	Hericium erinaceus polysaccharides (HEPs) have attracted widespread attention in regulating gut microbiota (GM).
35433782	1	48	theme	erinaceus	180:188	arg1	polysaccharides					190:204	Hericium erinaceus polysaccharides	171:204	Hericium erinaceus polysaccharides (HEPs)	171:211	Hericium erinaceus polysaccharides (HEPs) have attracted widespread attention in regulating gut microbiota (GM).
35433782	2	49	from	composition	362:372	arg1	fermentation					317:328	fermentation	317:328	fermentation	317:328	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	49	from	composition	362:372	arg1	digestibility					299:311	digestibility	299:311	digestibility	299:311	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	50	theme	HEPs	333:336	arg1	fermentation					317:328	fermentation	317:328	fermentation	317:328	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	50	theme	HEPs	333:336	arg1	digestibility					299:311	digestibility	299:311	digestibility	299:311	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	51	theme	erinaceus	570:578	arg1	extracts					549:556	hot water-soluble extracts	531:556	hot water-soluble extracts of Hericium erinaceus	531:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	3	52	theme	microbiota	731:740	arg1	fermentations					742:754	human fecal microbiota fermentations	719:754	human fecal microbiota fermentations	719:754	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	0	53	theme	Volunteers	130:139	arg1	Microbiota					100:109	Fecal Microbiota	94:109	Fecal Microbiota of Male and Female Volunteers	94:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	8	54	theme	polysaccharides	1646:1660	arg1	preparation					1631:1641	the preparation	1627:1641	the preparation of polysaccharides from Hericium erinaceus	1627:1684	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	3	55	theme	human	719:723	arg1	fermentations					742:754	human fecal microbiota fermentations	719:754	human fecal microbiota fermentations	719:754	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	0	56	theme	in	148:149	arg1	Fermentation					157:168	in vitro Fermentation	148:168	in vitro Fermentation	148:168	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	8	57	theme	Hericium	1667:1674	arg1	erinaceus					1676:1684	Hericium erinaceus	1667:1684	Hericium erinaceus	1667:1684	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	2	58	theme	%	495:495	arg1	concentrations					505:518	70% ethanol concentrations	493:518	70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus	493:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	58	theme	%	495:495	arg1	v/v					521:523	v/v	521:523	v/v	521:523	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	0	59	from	Effects	83:89	arg1	Microbiota					100:109	Fecal Microbiota	94:109	Fecal Microbiota of Male and Female Volunteers	94:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	3	60	theme	fatty	791:795	arg1	SCFA					803:806	SCFA	803:806	SCFA	803:806	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	3	60	theme	fatty	791:795	arg1	acid					797:800	short-chain fatty acid	779:800	short-chain fatty acid (SCFA) production in vitro	779:827	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	6	61	theme	relative	1277:1284	arg1	abundance					1286:1294	the relative abundance	1273:1294	the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus	1273:1406	Thus, HEPs could be utilized by GM, especially HEP-50, and enhanced the relative abundance of SCFA-producing bacteria, e.g., Bifidobacterium, Faecalibacterium, Blautia, Butyricicoccus, and Lactobacillus.
35433782	0	62	dep	in	148:149	arg1	vitro					151:155	vitro	151:155	vitro	151:155	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	7	63	theme	bacteria	1487:1494	arg1	abundances					1448:1457	the relative abundances	1435:1457	the relative abundances of opportunistic pathogenic bacteria	1435:1494	Furthermore, HEPs reduced the relative abundances of opportunistic pathogenic bacteria, e.g., Escherichia-Shigella, Klebsiella, and Enterobacter.
35433782	0	64	theme	Fecal	94:98	arg1	Microbiota					100:109	Fecal Microbiota	94:109	Fecal Microbiota of Male and Female Volunteers	94:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	2	65	with	precipitated	461:472	arg1	concentrations					505:518	70% ethanol concentrations	493:518	70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus	493:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	65	with	precipitated	461:472	arg1	v/v					521:523	v/v	521:523	v/v	521:523	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	65	with	precipitated	461:472	arg1	%					481:481	30%	479:481	30%	479:481	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	65	with	precipitated	461:472	arg1	%					486:486	50%	484:486	50%	484:486	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	7	66	theme	opportunistic	1462:1474	arg1	bacteria					1487:1494	opportunistic pathogenic bacteria	1462:1494	opportunistic pathogenic bacteria	1462:1494	Furthermore, HEPs reduced the relative abundances of opportunistic pathogenic bacteria, e.g., Escherichia-Shigella, Klebsiella, and Enterobacter.
35433782	0	67	from	Characteristics	10:24	arg1	Microbiota					100:109	Fecal Microbiota	94:109	Fecal Microbiota of Male and Female Volunteers	94:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	5	68	theme	SCFAs	1053:1057	arg1	content					1042:1048	the content	1038:1048	the content of SCFAs	1038:1057	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	8	69	theme	great	1764:1768	arg1	value					1782:1786	great development value	1764:1786	great development value	1764:1786	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	2	70	theme	ethanol	497:503	arg1	concentrations					505:518	70% ethanol concentrations	493:518	70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus	493:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	70	theme	ethanol	497:503	arg1	v/v					521:523	v/v	521:523	v/v	521:523	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	71	from	extracts	549:556	arg1	concentrations					505:518	70% ethanol concentrations	493:518	70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus	493:578	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	71	from	extracts	549:556	arg1	v/v					521:523	v/v	521:523	v/v	521:523	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	71	from	extracts	549:556	arg1	%					481:481	30%	479:481	30%	479:481	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	71	from	extracts	549:556	arg1	%					486:486	50%	484:486	50%	484:486	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	72	theme	polysaccharide	381:394	arg1	HEP-70					435:440	HEP-70	435:440	HEP-70	435:440	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	72	theme	polysaccharide	381:394	arg1	HEP-30					415:420	HEP-30	415:420	HEP-30	415:420	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	72	theme	polysaccharide	381:394	arg1	fractions					396:404	three polysaccharide fractions	375:404	three polysaccharide fractions	375:404	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	72	theme	polysaccharide	381:394	arg1	HEP-50					423:428	HEP-50	423:428	HEP-50	423:428	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	0	73	theme	Hericium	29:36	arg1	Polysaccharides					48:62	Hericium erinaceus Polysaccharides	29:62	Hericium erinaceus Polysaccharides	29:62	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	5	74	theme	retention	1106:1114	arg1	rates					1116:1120	the retention rates	1102:1120	the retention rates of total and reducing sugars and pH value	1102:1162	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	5	75	dep	enhanced	1077:1084	arg1	<					1089:1089	p < 0.05	1087:1094	p < 0.05	1087:1094	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	0	76	theme	Polysaccharides	48:62	arg1	Characteristics					10:24	Digestive Characteristics	0:24	Digestive Characteristics of Hericium erinaceus Polysaccharides	0:62	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	0	76	theme	Polysaccharides	48:62	arg1	Effects					83:89	Their Positive Effects	68:89	Their Positive Effects on Fecal Microbiota of Male and Female Volunteers	68:139	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	7	77	theme	relative	1439:1446	arg1	abundances					1448:1457	the relative abundances	1435:1457	the relative abundances of opportunistic pathogenic bacteria	1435:1494	Furthermore, HEPs reduced the relative abundances of opportunistic pathogenic bacteria, e.g., Escherichia-Shigella, Klebsiella, and Enterobacter.
35433782	8	78	from	preparation	1631:1641	arg1	erinaceus					1676:1684	Hericium erinaceus	1667:1684	Hericium erinaceus	1667:1684	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	2	79	theme	effects	348:354	arg1	fermentation					317:328	fermentation	317:328	fermentation	317:328	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	2	79	theme	effects	348:354	arg1	digestibility					299:311	digestibility	299:311	digestibility	299:311	To investigate digestibility and fermentation of HEPs and their effects on GM composition, three polysaccharide fractions, namely, HEP-30, HEP-50, and HEP-70, were fractionally precipitated with 30%, 50%, and 70% ethanol concentrations (v/v) from hot water-soluble extracts of Hericium erinaceus, respectively.
35433782	3	80	theme	HEPs	619:622	arg1	kinds					601:605	Three kinds	595:605	Three kinds of prepared HEPs	595:622	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	8	81	theme	gradual	1580:1586	arg1	precipitation					1596:1608	gradual ethanol precipitation	1580:1608	gradual ethanol precipitation	1580:1608	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	5	82	theme	pH	1155:1156	arg1	value					1158:1162	pH value	1155:1162	pH value	1155:1162	After 24 h of in vitro fermentation, the content of SCFAs was significantly enhanced (p < 0.05), and the retention rates of total and reducing sugars and pH value were significantly decreased (p < 0.05).
35433782	0	83	dep	Characteristics	10:24	arg1	Fermentation					157:168	in vitro Fermentation	148:168	in vitro Fermentation	148:168	Digestive Characteristics of Hericium erinaceus Polysaccharides and Their Positive Effects on Fecal Microbiota of Male and Female Volunteers During in vitro Fermentation.
35433782	8	84	with	foods	1753:1757	arg1	value					1782:1786	great development value	1764:1786	great development value	1764:1786	This study suggests that gradual ethanol precipitation is available for the preparation of polysaccharides from Hericium erinaceus, and the extracted polysaccharide could be developed as functional foods with great development value.
35433782	3	85	theme	gastrointestinal	670:685	arg1	digestion					687:695	simulated gastrointestinal digestion	660:695	simulated gastrointestinal digestion	660:695	Three kinds of prepared HEPs were structurally characterized and simulated gastrointestinal digestion, and their effects on human fecal microbiota fermentations of male and female and short-chain fatty acid (SCFA) production in vitro were clarified.
35433782	1	86	theme	gut	263:265	arg1	GM					279:280	GM	279:280	GM	279:280	Hericium erinaceus polysaccharides (HEPs) have attracted widespread attention in regulating gut microbiota (GM).
35433782	1	86	theme	gut	263:265	arg1	microbiota					267:276	gut microbiota	263:276	gut microbiota (GM)	263:281	Hericium erinaceus polysaccharides (HEPs) have attracted widespread attention in regulating gut microbiota (GM).
35889192	0	0	theme	Dictyosphaerium	87:101	arg1	Algae					81:85	the Green Algae	71:85	the Green Algae Dictyosphaerium chlorelloides	71:115	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	7	1	theme	Pilot-scale	997:1007	arg1	cultivation					1009:1019	Pilot-scale cultivation	997:1019	Pilot-scale cultivation	997:1019	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	11	2	theme	commercial	1520:1529	arg1	production					1531:1540	the commercial production	1516:1540	the commercial production of EPS	1516:1547	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	1	3	theme	freshwater	173:182	arg1	microalgae					190:199	The freshwater green microalgae	169:199	The freshwater green microalgae	169:199	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	3	theme	freshwater	173:182	arg1	chlorelloides					218:230	Dictyosphaerium chlorelloides	202:230	Dictyosphaerium chlorelloides (CCALA 330)	202:242	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	5	4	from	production	785:794	arg1	medium					799:804	medium	799:804	medium containing ammonium nitrate	799:832	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	3	5	theme	EPS	544:546	arg1	overproduction					548:561	EPS overproduction	544:561	EPS overproduction	544:561	Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction.
35889192	9	6	theme	strong	1237:1242	arg1	attachment					1244:1253	the strong attachment	1233:1253	the strong attachment between cells and EPS	1233:1275	Due to the strong attachment between cells and EPS, high-pressure homogenization was carried out before a centrifugation process.
35889192	2	7	theme	optimum	324:330	arg1	growth					332:337	optimum growth	324:337	optimum growth	324:337	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	7	8	theme	working	1096:1102	arg1	volume					1104:1109	a working volume	1094:1109	a working volume of 60 L	1094:1117	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	1	9	theme	extracellular	272:284	arg1	EPS					303:305	EPS	303:305	EPS	303:305	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	9	theme	extracellular	272:284	arg1	polysaccharides					286:300	extracellular polysaccharides	272:300	extracellular polysaccharides (EPS)	272:306	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	7	10	theme	type	1054:1057	arg1	PBR					1084:1086	PBR	1084:1086	PBR	1084:1086	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	7	10	theme	type	1054:1057	arg1	photobioreactor					1067:1081	a column type airlift photobioreactor	1045:1081	a column type airlift photobioreactor (PBR) with a working volume of 60 L	1045:1117	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	8	11	theme	EPS-containing	1196:1209	arg1	broth					1219:1223	the EPS-containing culture broth	1192:1223	the EPS-containing culture broth	1192:1223	A new and efficient methodology was developed for separating cells from the EPS-containing culture broth.
35889192	1	12	theme	green	184:188	arg1	microalgae					190:199	The freshwater green microalgae	169:199	The freshwater green microalgae	169:199	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	12	theme	green	184:188	arg1	chlorelloides					218:230	Dictyosphaerium chlorelloides	202:230	Dictyosphaerium chlorelloides (CCALA 330)	202:242	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	6	13	theme	g/L	958:960	arg1	biomass					944:950	a total biomass	936:950	a total biomass of 14 g/L	936:960	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	6	13	theme	g/L	958:960	arg1	g/L					901:903	10 g/L	898:903	10 g/L (71% of the total biomass)	898:930	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	5	14	theme	EPS	781:783	arg1	production					785:794	EPS production	781:794	EPS production	781:794	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	9	15	theme	centrifugation	1332:1345	arg1	process					1347:1353	a centrifugation process	1330:1353	a centrifugation process	1330:1353	Due to the strong attachment between cells and EPS, high-pressure homogenization was carried out before a centrifugation process.
35889192	2	16	theme	mL	458:459	arg1	volume					444:449	a working volume	434:449	a working volume of 300 mL	434:459	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	0	17	dep	Dictyosphaerium	87:101	arg1	chlorelloides					103:115	chlorelloides	103:115	chlorelloides	103:115	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	7	18	theme	L	1117:1117	arg1	volume					1104:1109	a working volume	1094:1109	a working volume of 60 L	1094:1117	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	5	19	theme	ammonium	817:824	arg1	nitrate					826:832	ammonium nitrate	817:832	ammonium nitrate	817:832	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	4	20	theme	Salinity	564:571	arg1	stress					573:578	Salinity stress	564:578	Salinity stress	564:578	Salinity stress was also applied to the culture, but no significant increase in EPS production was observed.
35889192	7	21	theme	column	1047:1052	arg1	PBR					1084:1086	PBR	1084:1086	PBR	1084:1086	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	7	21	theme	column	1047:1052	arg1	photobioreactor					1067:1081	a column type airlift photobioreactor	1045:1081	a column type airlift photobioreactor (PBR) with a working volume of 60 L	1045:1117	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	1	22	theme	Dictyosphaerium	202:216	arg1	microalgae					190:199	The freshwater green microalgae	169:199	The freshwater green microalgae	169:199	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	22	theme	Dictyosphaerium	202:216	arg1	chlorelloides					218:230	Dictyosphaerium chlorelloides	202:230	Dictyosphaerium chlorelloides (CCALA 330)	202:242	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	22	theme	Dictyosphaerium	202:216	arg1	330					239:241	CCALA 330	233:241	CCALA 330	233:241	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	8	23	theme	efficient	1130:1138	arg1	methodology					1140:1150	A new and efficient methodology	1120:1150	A new and efficient methodology	1120:1150	A new and efficient methodology was developed for separating cells from the EPS-containing culture broth.
35889192	5	24	theme	nitrogen	698:705	arg1	sources					707:713	different nitrogen sources	688:713	different nitrogen sources	688:713	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	11	25	theme	EPS	1545:1547	arg1	production					1531:1540	the commercial production	1516:1540	the commercial production of EPS	1516:1547	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	2	26	theme	working	436:442	arg1	volume					444:449	a working volume	434:449	a working volume of 300 mL	434:459	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	9	27	theme	high-pressure	1278:1290	arg1	homogenization					1292:1305	high-pressure homogenization	1278:1305	high-pressure homogenization	1278:1305	Due to the strong attachment between cells and EPS, high-pressure homogenization was carried out before a centrifugation process.
35889192	5	28	theme	sources	707:713	arg1	effects					677:683	The effects	673:683	The effects of different nitrogen sources	673:713	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	4	29	from	increase	632:639	arg1	production					648:657	EPS production	644:657	EPS production	644:657	Salinity stress was also applied to the culture, but no significant increase in EPS production was observed.
35889192	4	30	theme	significant	620:630	arg1	increase					632:639	no significant increase	617:639	no significant increase in EPS production	617:657	Salinity stress was also applied to the culture, but no significant increase in EPS production was observed.
35889192	0	31	theme	Extracellular	38:50	arg1	Polysaccharides					52:66	Extracellular Polysaccharides	38:66	Extracellular Polysaccharides	38:66	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	6	32	theme	total	938:942	arg1	biomass					944:950	a total biomass	936:950	a total biomass of 14 g/L	936:960	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	4	33	theme	EPS	644:646	arg1	production					648:657	EPS production	644:657	EPS production	644:657	Salinity stress was also applied to the culture, but no significant increase in EPS production was observed.
35889192	8	34	theme	new	1122:1124	arg1	methodology					1140:1150	A new and efficient methodology	1120:1150	A new and efficient methodology	1120:1150	A new and efficient methodology was developed for separating cells from the EPS-containing culture broth.
35889192	2	35	theme	tubular	398:404	arg1	PBR					424:426	PBR	424:426	PBR	424:426	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	2	35	theme	tubular	398:404	arg1	photobioreactors					406:421	laboratory-scale tubular photobioreactors	381:421	laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL	381:459	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	1	36	theme	CCALA	233:237	arg1	chlorelloides					218:230	Dictyosphaerium chlorelloides	202:230	Dictyosphaerium chlorelloides (CCALA 330)	202:242	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	36	theme	CCALA	233:237	arg1	330					239:241	CCALA 330	233:241	CCALA 330	233:241	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	6	37	dep	g/L	901:903	arg1	biomass					923:929	the total biomass	913:929	the total biomass	913:929	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	6	37	dep	g/L	901:903	arg1	%					908:908	71%	906:908	71% of the total biomass	906:929	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	6	38	theme	EPS	872:874	arg1	concentration					876:888	EPS concentration	872:888	EPS concentration	872:888	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	1	39	contain	has	245:247	arg1	microalgae					190:199	The freshwater green microalgae	169:199	The freshwater green microalgae	169:199	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	39	contain	has	245:247	arg1	chlorelloides					218:230	Dictyosphaerium chlorelloides	202:230	Dictyosphaerium chlorelloides (CCALA 330)	202:242	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	1	39	contain	has	245:247	arg2	ability					253:259	the ability to produce extracellular polysaccharides (EPS)	249:306	the ability to produce extracellular polysaccharides (EPS)	249:306	The freshwater green microalgae, Dictyosphaerium chlorelloides (CCALA 330), has the ability to produce extracellular polysaccharides (EPS).
35889192	3	40	theme	Multiple	462:469	arg1	method					533:538	an effective method	520:538	an effective method for EPS overproduction	520:561	Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction.
35889192	3	40	theme	Multiple	462:469	arg1	limitations					471:481	Multiple limitations	462:481	Multiple limitations in nutrient supply	462:500	Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction.
35889192	5	41	contain	containing	806:815	arg1	medium					799:804	medium	799:804	medium containing ammonium nitrate	799:832	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	5	41	contain	containing	806:815	arg2	nitrate					826:832	ammonium nitrate	817:832	ammonium nitrate	817:832	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	7	42	with	photobioreactor	1067:1081	arg1	volume					1104:1109	a working volume	1094:1109	a working volume of 60 L	1094:1117	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	0	43	theme	Polysaccharides	52:66	arg1	Production					24:33	the Production	20:33	the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides	20:115	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	0	43	theme	Polysaccharides	52:66	arg1	Purification					138:149	Purification	138:149	Purification	138:149	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	0	43	theme	Polysaccharides	52:66	arg1	Composition					156:166	Composition	156:166	Composition	156:166	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	0	43	theme	Polysaccharides	52:66	arg1	Isolation					127:135	Their Isolation	121:135	Their Isolation	121:135	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	6	44	theme	total	917:921	arg1	biomass					923:929	the total biomass	913:929	the total biomass	913:929	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	5	45	from	growth	770:775	arg1	medium					799:804	medium	799:804	medium containing ammonium nitrate	799:832	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	7	46	theme	airlift	1059:1065	arg1	PBR					1084:1086	PBR	1084:1086	PBR	1084:1086	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	7	46	theme	airlift	1059:1065	arg1	photobioreactor					1067:1081	a column type airlift photobioreactor	1045:1081	a column type airlift photobioreactor (PBR) with a working volume of 60 L	1045:1117	Pilot-scale cultivation was also carried out in a column type airlift photobioreactor (PBR) with a working volume of 60 L.
35889192	3	47	from	limitations	471:481	arg1	supply					495:500	nutrient supply	486:500	nutrient supply	486:500	Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction.
35889192	2	48	theme	EPS	343:345	arg1	overproduction					347:360	EPS overproduction	343:360	EPS overproduction	343:360	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	11	49	theme	Dictyosphaerium	1453:1467	arg1	chlorelloides					1469:1481	The green microalgae Dictyosphaerium chlorelloides	1432:1481	The green microalgae Dictyosphaerium chlorelloides	1432:1481	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	11	49	theme	Dictyosphaerium	1453:1467	arg1	appropriate					1500:1510	appropriate	1500:1510	appropriate	1500:1510	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	6	50	theme	biomass	923:929	arg1	biomass					923:929	the total biomass	913:929	the total biomass	913:929	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	6	50	theme	biomass	923:929	arg1	%					908:908	71%	906:908	71% of the total biomass	906:929	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	6	51	theme	optimal	852:858	arg1	conditions					860:869	determined optimal conditions	841:869	determined optimal conditions	841:869	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	3	52	theme	nutrient	486:493	arg1	supply					495:500	nutrient supply	486:500	nutrient supply	486:500	Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction.
35889192	10	53	from	EPS	1360:1362	arg1	supernatant					1371:1381	the supernatant	1367:1381	the supernatant	1367:1381	The EPS in the supernatant was subsequently purified using ultrafiltration.
35889192	6	54	theme	cultivation	984:994	arg1	end					969:971	the end	965:971	the end of 17 days cultivation	965:994	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	5	55	theme	different	688:696	arg1	sources					707:713	different nitrogen sources	688:713	different nitrogen sources	688:713	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	6	56	theme	determined	841:850	arg1	conditions					860:869	determined optimal conditions	841:869	determined optimal conditions	841:869	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	11	57	theme	green	1436:1440	arg1	chlorelloides					1469:1481	The green microalgae Dictyosphaerium chlorelloides	1432:1481	The green microalgae Dictyosphaerium chlorelloides	1432:1481	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	11	57	theme	green	1436:1440	arg1	appropriate					1500:1510	appropriate	1500:1510	appropriate	1500:1510	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	6	58	theme	days	979:982	arg1	cultivation					984:994	17 days cultivation	976:994	17 days cultivation	976:994	Under determined optimal conditions, EPS concentration reached 10 g/L (71% of the total biomass) and a total biomass of 14 g/L at the end of 17 days cultivation.
35889192	0	59	theme	Green	75:79	arg1	Algae					81:85	the Green Algae	71:85	the Green Algae Dictyosphaerium chlorelloides	71:115	Factors Influencing the Production of Extracellular Polysaccharides by the Green Algae Dictyosphaerium chlorelloides and Their Isolation, Purification, and Composition.
35889192	8	60	theme	culture	1211:1217	arg1	broth					1219:1223	the EPS-containing culture broth	1192:1223	the EPS-containing culture broth	1192:1223	A new and efficient methodology was developed for separating cells from the EPS-containing culture broth.
35889192	2	61	theme	laboratory-scale	381:396	arg1	PBR					424:426	PBR	424:426	PBR	424:426	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	2	61	theme	laboratory-scale	381:396	arg1	photobioreactors					406:421	laboratory-scale tubular photobioreactors	381:421	laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL	381:459	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	11	62	theme	microalgae	1442:1451	arg1	chlorelloides					1469:1481	The green microalgae Dictyosphaerium chlorelloides	1432:1481	The green microalgae Dictyosphaerium chlorelloides	1432:1481	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	11	62	theme	microalgae	1442:1451	arg1	appropriate					1500:1510	appropriate	1500:1510	appropriate	1500:1510	The green microalgae Dictyosphaerium chlorelloides may therefore be appropriate for the commercial production of EPS.
35889192	5	63	theme	fastest	762:768	arg1	growth					770:775	fastest growth	762:775	fastest growth	762:775	The effects of different nitrogen sources were examined and the microalgae exhibited the fastest growth and EPS production in medium containing ammonium nitrate.
35889192	2	64	with	photobioreactors	406:421	arg1	volume					444:449	a working volume	434:449	a working volume of 300 mL	434:459	Conditions for optimum growth and EPS overproduction were determined in laboratory-scale tubular photobioreactors (PBR) with a working volume of 300 mL.
35889192	3	65	theme	effective	523:531	arg1	method					533:538	an effective method	520:538	an effective method for EPS overproduction	520:561	Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction.
35889192	3	65	theme	effective	523:531	arg1	limitations					471:481	Multiple limitations	462:481	Multiple limitations in nutrient supply	462:500	Multiple limitations in nutrient supply were proven to be an effective method for EPS overproduction.
36305787	3	0	theme	controlled	731:740	arg1	enrichment					747:756	controlled soil enrichment	731:756	controlled soil enrichment	731:756	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	1	1	theme	water	286:290	arg1	production					292:301	clean water production	280:301	clean water production	280:301	Solar-powered interfacial evaporation has emerged as an innovative and sustainable technology for clean water production.
36305787	9	2	from	germination	1520:1530	arg1	stage					1561:1565	a whole growth stage	1546:1565	a whole growth stage	1546:1565	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	5	3	theme	kg	999:1000	arg1	h-1					1006:1008	1.62 kg m-2 h-1	994:1008	1.62 kg m-2 h-1	994:1008	The composite beads can attain a high water evaporation rate of 1.62 kg m-2 h-1 under 1 sun.
36305787	10	4	dep	mass	1598:1601	arg1	the					1594:1596	the	1594:1596	the	1594:1596	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	4	5	theme	ion	914:916	arg1	adsorption					918:927	enhanced ion adsorption	905:927	enhanced ion adsorption	905:927	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	6	6	theme	broad	1103:1107	arg1	ranges					1159:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges	1103:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions	1103:1187	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	1	7	theme	interfacial	196:206	arg1	evaporation					208:218	Solar-powered interfacial evaporation	182:218	Solar-powered interfacial evaporation	182:218	Solar-powered interfacial evaporation has emerged as an innovative and sustainable technology for clean water production.
36305787	6	8	theme	phosphate	1169:1177	arg1	solutions					1179:1187	phosphate solutions	1169:1187	phosphate solutions	1169:1187	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	4	9	with	aerogels	848:855	arg1	adsorption					918:927	enhanced ion adsorption	905:927	enhanced ion adsorption	905:927	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	4	9	with	aerogels	848:855	arg1	processing					889:898	continuous processing	878:898	continuous processing	878:898	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	4	9	with	aerogels	848:855	arg1	gelation					868:875	rapid gelation	862:875	rapid gelation	862:875	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	3	10	dep	hydrophilic	536:546	arg1	i.e.					530:533	i.e.	530:533	i.e.	530:533	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	5	11	theme	m-2	1002:1004	arg1	h-1					1006:1008	1.62 kg m-2 h-1	994:1008	1.62 kg m-2 h-1	994:1008	The composite beads can attain a high water evaporation rate of 1.62 kg m-2 h-1 under 1 sun.
36305787	10	12	theme	water	1765:1769	arg1	remediation					1771:1781	water remediation	1765:1781	water remediation	1765:1781	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	9	13	dep	Macronutrients	1435:1448	arg1	K					1461:1461	K	1461:1461	K	1461:1461	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	9	13	dep	Macronutrients	1435:1448	arg1	N					1451:1451	N	1451:1451	N	1451:1451	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	9	13	dep	Macronutrients	1435:1448	arg1	Macronutrients					1435:1448	Macronutrients	1435:1448	Macronutrients (N, P, and K)	1435:1462	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	9	13	dep	Macronutrients	1435:1448	arg1	P					1454:1454	P	1454:1454	P	1454:1454	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	3	14	theme	threefold	619:627	arg1	evaporation					695:705	efficient solar-powered interfacial evaporation	659:705	efficient solar-powered interfacial evaporation	659:705	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	14	theme	threefold	619:627	arg1	applications					636:647	threefold hybrid applications	619:647	threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment	619:756	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	14	theme	threefold	619:627	arg1	enrichment					747:756	controlled soil enrichment	731:756	controlled soil enrichment	731:756	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	14	theme	threefold	619:627	arg1	remediation					714:724	water remediation	708:724	water remediation	708:724	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	1	15	theme	innovative	238:247	arg1	technology					265:274	an innovative and sustainable technology	235:274	an innovative and sustainable technology for clean water production	235:301	Solar-powered interfacial evaporation has emerged as an innovative and sustainable technology for clean water production.
36305787	0	16	from	Fabrication	64:74	arg1	Remediation					148:158	Water Remediation	142:158	Water Remediation	142:158	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	0	16	from	Fabrication	64:74	arg1	Enrichment					170:179	Soil Enrichment	165:179	Soil Enrichment	165:179	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	0	16	from	Fabrication	64:74	arg1	Evaporation					129:139	Solar-Powered Interfacial Evaporation	103:139	Solar-Powered Interfacial Evaporation	103:139	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	4	17	theme	rational	763:770	arg1	incorporation					772:784	The rational incorporation	759:784	The rational incorporation of selected polysaccharides	759:812	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	10	18	theme	material	1790:1797	arg1	disposal					1799:1806	waste material disposal	1784:1806	waste material disposal	1784:1806	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	3	19	theme	efficient	659:667	arg1	evaporation					695:705	efficient solar-powered interfacial evaporation	659:705	efficient solar-powered interfacial evaporation	659:705	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	7	20	theme	biomaterials	1250:1261	arg1	example					1229:1235	the first example	1219:1235	the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture	1219:1360	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	10	21	theme	value-added	1813:1823	arg1	conversion					1825:1834	value-added conversion	1813:1834	value-added conversion	1813:1834	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	0	22	theme	Hybrid	80:85	arg1	Applications					87:98	Hybrid Applications	80:98	Hybrid Applications	80:98	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	5	23	theme	water	968:972	arg1	rate					986:989	a high water evaporation rate	961:989	a high water evaporation rate of 1.62 kg m-2 h-1	961:1008	The composite beads can attain a high water evaporation rate of 1.62 kg m-2 h-1 under 1 sun.
36305787	6	24	theme	phosphate	1039:1047	arg1	capacity					1060:1067	high phosphate adsorption capacity	1034:1067	high phosphate adsorption capacity of over 120 mg g-1	1034:1086	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	6	24	theme	phosphate	1039:1047	arg1	Meanwhile					1023:1031	Meanwhile	1023:1031	Meanwhile	1023:1031	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	6	25	dep	g-1	1084:1086	arg1	mg					1081:1082	mg	1081:1082	mg	1081:1082	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	7	26	theme	first	1223:1227	arg1	example					1229:1235	the first example	1219:1235	the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture	1219:1360	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	3	27	dep	properties	518:527	arg1	hydrophilic					536:546	hydrophilic	536:546	hydrophilic	536:546	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	27	dep	properties	518:527	arg1	photothermal					557:568	photothermal	557:568	photothermal	557:568	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	27	dep	properties	518:527	arg1	durable					575:581	durable	575:581	durable	575:581	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	27	dep	properties	518:527	arg1	porous					549:554	porous	549:554	porous	549:554	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	10	28	theme	aerogel	1633:1639	arg1	beads					1641:1645	aerogel beads	1633:1645	aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion	1633:1834	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	0	29	theme	Interfacial	117:127	arg1	Evaporation					129:139	Solar-Powered Interfacial Evaporation	103:139	Solar-Powered Interfacial Evaporation	103:139	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	6	30	theme	pH	1109:1110	arg1	ranges					1159:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges	1103:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions	1103:1187	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	4	31	theme	polysaccharides	798:812	arg1	incorporation					772:784	The rational incorporation	759:784	The rational incorporation of selected polysaccharides	759:812	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	0	32	theme	Photothermal	0:11	arg1	Beads					21:25	Photothermal Aerogel Beads	0:25	Photothermal Aerogel Beads	0:25	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	9	33	theme	growth	1554:1559	arg1	stage					1561:1565	a whole growth stage	1546:1565	a whole growth stage	1546:1565	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	10	34	theme	double-network	1656:1669	arg1	biopolymers					1671:1681	double-network biopolymers	1656:1681	double-network biopolymers	1656:1681	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	2	35	theme	hybrid	425:430	arg1	applications					432:443	versatile hybrid applications	415:443	versatile hybrid applications	415:443	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	7	36	theme	soil	1317:1320	arg1	enrichment					1322:1331	soil enrichment	1317:1331	soil enrichment	1317:1331	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	3	37	theme	composite	474:482	arg1	beads					492:496	composite aerogel beads	474:496	composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable)	474:582	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	38	theme	interfacial	683:693	arg1	evaporation					695:705	efficient solar-powered interfacial evaporation	659:705	efficient solar-powered interfacial evaporation	659:705	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	6	39	theme	mg	1151:1152	arg1	pH					1109:1110	pH (2.5-12.4)	1109:1121	pH (2.5-12.4)	1109:1121	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	6	39	theme	mg	1151:1152	arg1	L-1					1154:1156	200-1000 mg L-1	1142:1156	200-1000 mg L-1	1142:1156	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	4	40	theme	rapid	862:866	arg1	gelation					868:875	rapid gelation	862:875	rapid gelation	862:875	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	10	41	dep	beads	1641:1645	arg1	based					1647:1651	based	1647:1651	based on double-network biopolymers	1647:1681	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	10	41	dep	beads	1641:1645	arg1	considering					1753:1763	considering	1753:1763	considering water remediation, waste material disposal, and value-added conversion	1753:1834	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	10	41	dep	beads	1641:1645	arg1	scaling					1695:1701	scaling	1695:1701	scaling up solar-powered interfacial evaporation	1695:1742	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	3	42	theme	self-contained	503:516	arg1	properties					518:527	self-contained properties	503:527	self-contained properties (i.e., hydrophilic, porous, photothermal, and durable)	503:582	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	7	43	theme	nutrient	1299:1306	arg1	release					1308:1314	controlled nutrient release	1288:1314	controlled nutrient release	1288:1314	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	2	44	theme	versatile	415:423	arg1	applications					432:443	versatile hybrid applications	415:443	versatile hybrid applications	415:443	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	2	45	theme	generators	394:403	arg1	fabrication					350:360	the rapid, mass and shape-controlled fabrication	313:360	the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications	313:443	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	5	46	theme	composite	934:942	arg1	beads					944:948	The composite beads	930:948	The composite beads	930:948	The composite beads can attain a high water evaporation rate of 1.62 kg m-2 h-1 under 1 sun.
36305787	1	47	theme	clean	280:284	arg1	production					292:301	clean water production	280:301	clean water production	280:301	Solar-powered interfacial evaporation has emerged as an innovative and sustainable technology for clean water production.
36305787	7	48	theme	interfacial	1268:1278	arg1	SGs					1280:1282	interfacial SGs	1268:1282	interfacial SGs	1268:1282	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	3	49	theme	soil	742:745	arg1	enrichment					747:756	controlled soil enrichment	731:756	controlled soil enrichment	731:756	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	2	50	theme	rapid	317:321	arg1	fabrication					350:360	the rapid, mass and shape-controlled fabrication	313:360	the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications	313:443	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	10	51	theme	waste	1784:1788	arg1	disposal					1799:1806	waste material disposal	1784:1806	waste material disposal	1784:1806	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	7	52	from	SGs	1280:1282	arg1	example					1229:1235	the first example	1219:1235	the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture	1219:1360	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	7	52	from	SGs	1280:1282	arg1	biomaterials					1250:1261	recycling biomaterials	1240:1261	recycling biomaterials from interfacial SGs	1240:1282	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	9	53	theme	plant	1514:1518	arg1	germination					1520:1530	plant germination	1514:1530	plant germination	1514:1530	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	1	54	theme	Solar-powered	182:194	arg1	evaporation					208:218	Solar-powered interfacial evaporation	182:218	Solar-powered interfacial evaporation	182:218	Solar-powered interfacial evaporation has emerged as an innovative and sustainable technology for clean water production.
36305787	4	55	theme	enhanced	905:912	arg1	adsorption					918:927	enhanced ion adsorption	905:927	enhanced ion adsorption	905:927	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	6	56	theme	solutions	1179:1187	arg1	ranges					1159:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges	1103:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions	1103:1187	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	2	57	theme	shape-controlled	333:348	arg1	fabrication					350:360	the rapid, mass and shape-controlled fabrication	313:360	the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications	313:443	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	4	58	theme	selected	789:796	arg1	polysaccharides					798:812	selected polysaccharides	789:812	selected polysaccharides	789:812	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	0	59	theme	Water	142:146	arg1	Remediation					148:158	Water Remediation	142:158	Water Remediation	142:158	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	2	60	theme	mass	324:327	arg1	fabrication					350:360	the rapid, mass and shape-controlled fabrication	313:360	the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications	313:443	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	0	61	from	Applications	87:98	arg1	Remediation					148:158	Water Remediation	142:158	Water Remediation	142:158	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	0	61	from	Applications	87:98	arg1	Enrichment					170:179	Soil Enrichment	165:179	Soil Enrichment	165:179	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	0	61	from	Applications	87:98	arg1	Evaporation					129:139	Solar-Powered Interfacial Evaporation	103:139	Solar-Powered Interfacial Evaporation	103:139	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	3	62	with	beads	492:496	arg1	properties					518:527	self-contained properties	503:527	self-contained properties (i.e., hydrophilic, porous, photothermal, and durable)	503:582	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	0	63	theme	Soil	165:168	arg1	Enrichment					170:179	Soil Enrichment	165:179	Soil Enrichment	165:179	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	5	64	theme	evaporation	974:984	arg1	rate					986:989	a high water evaporation rate	961:989	a high water evaporation rate of 1.62 kg m-2 h-1	961:1008	The composite beads can attain a high water evaporation rate of 1.62 kg m-2 h-1 under 1 sun.
36305787	7	65	from	example	1229:1235	arg1	SGs					1280:1282	interfacial SGs	1268:1282	interfacial SGs	1268:1282	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	7	66	theme	recycling	1240:1248	arg1	biomaterials					1250:1261	recycling biomaterials	1240:1261	recycling biomaterials from interfacial SGs	1240:1282	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	3	67	theme	hybrid	629:634	arg1	evaporation					695:705	efficient solar-powered interfacial evaporation	659:705	efficient solar-powered interfacial evaporation	659:705	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	67	theme	hybrid	629:634	arg1	applications					636:647	threefold hybrid applications	619:647	threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment	619:756	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	67	theme	hybrid	629:634	arg1	enrichment					747:756	controlled soil enrichment	731:756	controlled soil enrichment	731:756	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	3	67	theme	hybrid	629:634	arg1	remediation					714:724	water remediation	708:724	water remediation	708:724	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	5	68	theme	high	963:966	arg1	rate					986:989	a high water evaporation rate	961:989	a high water evaporation rate of 1.62 kg m-2 h-1	961:1008	The composite beads can attain a high water evaporation rate of 1.62 kg m-2 h-1 under 1 sun.
36305787	6	69	theme	adsorption	1049:1058	arg1	capacity					1060:1067	high phosphate adsorption capacity	1034:1067	high phosphate adsorption capacity of over 120 mg g-1	1034:1086	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	6	69	theme	adsorption	1049:1058	arg1	Meanwhile					1023:1031	Meanwhile	1023:1031	Meanwhile	1023:1031	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	0	70	theme	Solar-Powered	103:115	arg1	Evaporation					129:139	Solar-Powered Interfacial Evaporation	103:139	Solar-Powered Interfacial Evaporation	103:139	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	9	71	from	growth	1536:1541	arg1	stage					1561:1565	a whole growth stage	1546:1565	a whole growth stage	1546:1565	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	6	72	theme	high	1034:1037	arg1	capacity					1060:1067	high phosphate adsorption capacity	1034:1067	high phosphate adsorption capacity of over 120 mg g-1	1034:1086	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	6	72	theme	high	1034:1037	arg1	Meanwhile					1023:1031	Meanwhile	1023:1031	Meanwhile	1023:1031	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	8	73	theme	phosphate-saturated	1367:1385	arg1	beads					1387:1391	The phosphate-saturated beads	1363:1391	The phosphate-saturated beads	1363:1391	The phosphate-saturated beads can be gradually broken down in the soil.
36305787	6	74	dep	mg	1081:1082	arg1	120					1077:1079	120	1077:1079	120	1077:1079	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	0	75	from	Polysaccharides	36:50	arg1	Remediation					148:158	Water Remediation	142:158	Water Remediation	142:158	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	0	75	from	Polysaccharides	36:50	arg1	Enrichment					170:179	Soil Enrichment	165:179	Soil Enrichment	165:179	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	0	75	from	Polysaccharides	36:50	arg1	Evaporation					129:139	Solar-Powered Interfacial Evaporation	103:139	Solar-Powered Interfacial Evaporation	103:139	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	4	76	theme	bead-like	838:846	arg1	aerogels					848:855	bead-like aerogels	838:855	bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption	838:927	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	0	77	theme	Aerogel	13:19	arg1	Beads					21:25	Photothermal Aerogel Beads	0:25	Photothermal Aerogel Beads	0:25	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	9	78	theme	whole	1548:1552	arg1	stage					1561:1565	a whole growth stage	1546:1565	a whole growth stage	1546:1565	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	7	79	theme	sustainable	1338:1348	arg1	agriculture					1350:1360	sustainable agriculture	1338:1360	sustainable agriculture	1338:1360	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	9	80	dep	germination	1520:1530	arg1	the					1510:1512	the	1510:1512	the	1510:1512	Macronutrients (N, P, and K) can be slowly released in 50 days, sustaining the plant germination and growth in a whole growth stage.
36305787	10	81	theme	controlled	1607:1616	arg1	fabrication					1618:1628	controlled fabrication	1607:1628	controlled fabrication	1607:1628	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	5	82	theme	h-1	1006:1008	arg1	rate					986:989	a high water evaporation rate	961:989	a high water evaporation rate of 1.62 kg m-2 h-1	961:1008	The composite beads can attain a high water evaporation rate of 1.62 kg m-2 h-1 under 1 sun.
36305787	10	83	theme	beads	1641:1645	arg1	mass					1598:1601	mass	1598:1601	mass	1598:1601	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	10	83	theme	beads	1641:1645	arg1	fabrication					1618:1628	controlled fabrication	1607:1628	controlled fabrication	1607:1628	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	2	84	dep	three-dimensional	365:381	arg1	3D					384:385	3D	384:385	3D	384:385	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	10	85	theme	interfacial	1720:1730	arg1	evaporation					1732:1742	solar-powered interfacial evaporation	1706:1742	solar-powered interfacial evaporation	1706:1742	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	6	86	theme	g-1	1084:1086	arg1	capacity					1060:1067	high phosphate adsorption capacity	1034:1067	high phosphate adsorption capacity of over 120 mg g-1	1034:1086	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	6	86	theme	g-1	1084:1086	arg1	Meanwhile					1023:1031	Meanwhile	1023:1031	Meanwhile	1023:1031	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	3	87	theme	solar-powered	669:681	arg1	evaporation					695:705	efficient solar-powered interfacial evaporation	659:705	efficient solar-powered interfacial evaporation	659:705	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	4	88	theme	continuous	878:887	arg1	processing					889:898	continuous processing	878:898	continuous processing	878:898	The rational incorporation of selected polysaccharides enables us to fabricate bead-like aerogels with rapid gelation, continuous processing, and enhanced ion adsorption.
36305787	3	89	theme	aerogel	484:490	arg1	beads					492:496	composite aerogel beads	474:496	composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable)	474:582	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	0	90	theme	Controlled	53:62	arg1	Fabrication					64:74	Controlled Fabrication	53:74	Controlled Fabrication	53:74	Photothermal Aerogel Beads Based on Polysaccharides: Controlled Fabrication and Hybrid Applications in Solar-Powered Interfacial Evaporation, Water Remediation, and Soil Enrichment.
36305787	2	91	theme	three-dimensional	365:381	arg1	SGs					406:408	SGs	406:408	SGs	406:408	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	2	91	theme	three-dimensional	365:381	arg1	generators					394:403	three-dimensional (3D) steam generators	365:403	three-dimensional (3D) steam generators (SGs)	365:409	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	7	92	theme	controlled	1288:1297	arg1	release					1308:1314	controlled nutrient release	1288:1314	controlled nutrient release	1288:1314	Gratifyingly, we demonstrate the first example of recycling biomaterials from interfacial SGs for controlled nutrient release, soil enrichment, and sustainable agriculture.
36305787	3	93	theme	water	708:712	arg1	remediation					714:724	water remediation	708:724	water remediation	708:724	Herein, composite aerogel beads with self-contained properties (i.e., hydrophilic, porous, photothermal, and durable) are developed and demonstrated for threefold hybrid applications including efficient solar-powered interfacial evaporation, water remediation, and controlled soil enrichment.
36305787	10	94	theme	solar-powered	1706:1718	arg1	evaporation					1732:1742	solar-powered interfacial evaporation	1706:1742	solar-powered interfacial evaporation	1706:1742	This work shines light on the mass and controlled fabrication of aerogel beads based on double-network biopolymers, not merely scaling up solar-powered interfacial evaporation but also considering water remediation, waste material disposal, and value-added conversion.
36305787	1	95	theme	sustainable	253:263	arg1	technology					265:274	an innovative and sustainable technology	235:274	an innovative and sustainable technology for clean water production	235:301	Solar-powered interfacial evaporation has emerged as an innovative and sustainable technology for clean water production.
36305787	6	96	theme	concentration	1127:1139	arg1	ranges					1159:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges	1103:1164	broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions	1103:1187	Meanwhile, high phosphate adsorption capacity of over 120 mg g-1 is achieved in broad pH (2.5-12.4) and concentration (200-1000 mg L-1) ranges of phosphate solutions.
36305787	2	97	theme	steam	388:392	arg1	SGs					406:408	SGs	406:408	SGs	406:408	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36305787	2	97	theme	steam	388:392	arg1	generators					394:403	three-dimensional (3D) steam generators	365:403	three-dimensional (3D) steam generators (SGs)	365:409	However, the rapid, mass and shape-controlled fabrication of three-dimensional (3D) steam generators (SGs) for versatile hybrid applications remains challenging.
36837842	4	0	theme	polysaccharides	690:704	arg1	composition					675:685	monosaccharide composition	660:685	monosaccharide composition of polysaccharides	660:704	The results showed that the molecular weight decreased and monosaccharide composition of polysaccharides changed significantly after fermentation.
36837842	7	1	theme	fatty	1376:1380	arg1	acids					1382:1386	unsaturated fatty acids	1364:1386	unsaturated fatty acids	1364:1386	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	2	2	theme	materials	337:345	arg1	activity					316:323	the functional activity	301:323	the functional activity of food raw materials	301:345	Fermentation has been considered as an effective way to promote the functional activity of food raw materials.
36837842	7	3	theme	acids	1382:1386	arg1	consistent					1398:1407	consistent	1398:1407	consistent	1398:1407	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	7	3	theme	acids	1382:1386	arg1	biosynthesis					1348:1359	the biosynthesis	1344:1359	the biosynthesis	1344:1359	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	8	4	theme	barley	1567:1572	arg1	bran					1574:1577	barley bran	1567:1577	barley bran	1567:1577	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	0	5	theme	Bran	83:86	arg1	Extract					88:94	Fermented Barley Bran Extract	66:94	Fermented Barley Bran Extract	66:94	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	2	6	theme	raw	333:335	arg1	materials					337:345	food raw materials	328:345	food raw materials	328:345	Fermentation has been considered as an effective way to promote the functional activity of food raw materials.
36837842	7	7	with	consistent	1398:1407	arg1	downregulation					1418:1431	the downregulation	1414:1431	the downregulation of scd-1 expression	1414:1451	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	0	8	theme	Lipid	99:103	arg1	Accumulation					105:116	Lipid Accumulation	99:116	Lipid Accumulation in HepG2 Cells	99:131	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	3	9	from	extract	396:402	arg1	polysaccharides					363:377	polysaccharides	363:377	polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS)	363:460	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	3	10	from	effects	485:491	arg1	accumulation					502:513	lipid accumulation	496:513	lipid accumulation	496:513	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	3	10	from	effects	485:491	arg1	stress					529:534	oxidative stress	519:534	oxidative stress	519:534	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	3	10	from	effects	485:491	arg1	cells					554:558	high-fat HepG2 cells	539:558	high-fat HepG2 cells induced by sodium oleate	539:583	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	2	11	theme	effective	276:284	arg1	Fermentation					237:248	Fermentation	237:248	Fermentation	237:248	Fermentation has been considered as an effective way to promote the functional activity of food raw materials.
36837842	2	11	theme	effective	276:284	arg1	way					286:288	an effective way	273:288	an effective way to promote the functional activity of food raw materials	273:345	Fermentation has been considered as an effective way to promote the functional activity of food raw materials.
36837842	5	12	theme	superoxide	956:965	arg1	dismutase					967:975	superoxide dismutase	956:975	superoxide dismutase (SOD)	956:981	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	5	12	theme	superoxide	956:965	arg1	SOD					978:980	SOD	978:980	SOD	978:980	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	5	13	from	content	813:819	arg1	cells					879:883	high-fat HepG2 cells	864:883	high-fat HepG2 cells	864:883	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	6	14	theme	metabolism-related	1117:1134	arg1	ppar-α					1150:1155	ppar-α	1150:1155	ppar-α	1150:1155	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	14	theme	metabolism-related	1117:1134	arg1	nrf2					1213:1216	nrf2	1213:1216	nrf2	1213:1216	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	14	theme	metabolism-related	1117:1134	arg1	cpt-1α					1169:1174	cpt-1α	1169:1174	cpt-1α	1169:1174	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	14	theme	metabolism-related	1117:1134	arg1	acox-1					1158:1163	acox-1	1158:1163	acox-1	1158:1163	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	14	theme	metabolism-related	1117:1134	arg1	genes					1136:1140	the lipid metabolism-related genes	1107:1140	the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α	1107:1174	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	1	15	theme	potential	150:158	arg1	bioactivities					160:172	potential bioactivities	150:172	potential bioactivities	150:172	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	1	16	theme	Barley	134:139	arg1	bran					141:144	Barley bran	134:144	Barley bran	134:144	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	8	17	dep	properties	1505:1514	arg1	the					1501:1503	the	1501:1503	the	1501:1503	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	18	theme	alleviating	1605:1615	arg1	effect					1617:1622	an alleviating effect	1602:1622	an alleviating effect on lipid deposition and oxidative stress in high-fat cells	1602:1681	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	6	19	theme	lipid	1111:1115	arg1	ppar-α					1150:1155	ppar-α	1150:1155	ppar-α	1150:1155	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	19	theme	lipid	1111:1115	arg1	nrf2					1213:1216	nrf2	1213:1216	nrf2	1213:1216	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	19	theme	lipid	1111:1115	arg1	cpt-1α					1169:1174	cpt-1α	1169:1174	cpt-1α	1169:1174	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	19	theme	lipid	1111:1115	arg1	acox-1					1158:1163	acox-1	1158:1163	acox-1	1158:1163	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	19	theme	lipid	1111:1115	arg1	genes					1136:1140	the lipid metabolism-related genes	1107:1140	the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α	1107:1174	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	3	20	theme	Lactiplantibacillus	417:435	arg1	FBBE-PS					453:459	FBBE-PS	453:459	FBBE-PS	453:459	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	3	20	theme	Lactiplantibacillus	417:435	arg1	dy-1					447:450	Lactiplantibacillus plantarum dy-1	417:450	Lactiplantibacillus plantarum dy-1 (FBBE-PS)	417:460	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	3	21	theme	barley	384:389	arg1	extract					396:402	barley bran extract	384:402	barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS)	384:460	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	1	22	contain	has	146:148	arg2	bioactivities					160:172	potential bioactivities	150:172	potential bioactivities	150:172	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	1	22	contain	has	146:148	arg1	bran					141:144	Barley bran	134:144	Barley bran	134:144	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	8	23	from	properties	1505:1514	arg1	bran					1574:1577	barley bran	1567:1577	barley bran	1567:1577	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	24	theme	physiological	1520:1532	arg1	activities					1534:1543	physiological activities	1520:1543	physiological activities	1520:1543	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	3	25	theme	plantarum	437:445	arg1	FBBE-PS					453:459	FBBE-PS	453:459	FBBE-PS	453:459	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	3	25	theme	plantarum	437:445	arg1	dy-1					447:450	Lactiplantibacillus plantarum dy-1	417:450	Lactiplantibacillus plantarum dy-1 (FBBE-PS)	417:460	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	5	26	theme	TG	809:810	arg1	content					813:819	the triglyceride (TG) content	791:819	the triglyceride (TG) content	791:819	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	0	27	from	Effect	35:40	arg1	Extract					88:94	Fermented Barley Bran Extract	66:94	Fermented Barley Bran Extract	66:94	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	0	27	from	Effect	35:40	arg1	Accumulation					105:116	Lipid Accumulation	99:116	Lipid Accumulation in HepG2 Cells	99:131	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	6	28	theme	oxidation-related	1181:1197	arg1	genes					1199:1203	oxidation-related genes	1181:1203	oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc	1181:1245	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	28	theme	oxidation-related	1181:1197	arg1	nrf2					1213:1216	nrf2	1213:1216	nrf2	1213:1216	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	28	theme	oxidation-related	1181:1197	arg1	ppar-α					1150:1155	ppar-α	1150:1155	ppar-α	1150:1155	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	28	theme	oxidation-related	1181:1197	arg1	cpt-1α					1169:1174	cpt-1α	1169:1174	cpt-1α	1169:1174	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	6	28	theme	oxidation-related	1181:1197	arg1	acox-1					1158:1163	acox-1	1158:1163	acox-1	1158:1163	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	3	29	theme	HepG2	548:552	arg1	cells					554:558	high-fat HepG2 cells	539:558	high-fat HepG2 cells induced by sodium oleate	539:583	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	8	30	theme	oxidative	1648:1656	arg1	stress					1658:1663	oxidative stress	1648:1663	oxidative stress	1648:1663	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	31	theme	high-fat	1668:1675	arg1	cells					1677:1681	high-fat cells	1668:1681	high-fat cells	1668:1681	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	1	32	theme	high	185:188	arg1	content					190:196	its high content	181:196	its high content of polyphenols and dietary fiber, etc	181:234	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	0	33	theme	Regulatory	24:33	arg1	Effect					35:40	the Regulatory Effect	20:40	the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells	20:131	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	8	34	from	polysaccharides	1548:1562	arg1	bran					1574:1577	barley bran	1567:1577	barley bran	1567:1577	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	35	from	deposition	1633:1642	arg1	cells					1677:1681	high-fat cells	1668:1681	high-fat cells	1668:1681	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	36	from	effect	1617:1622	arg1	deposition					1633:1642	lipid deposition	1627:1642	lipid deposition	1627:1642	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	36	from	effect	1617:1622	arg1	stress					1658:1663	oxidative stress	1648:1663	oxidative stress	1648:1663	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	37	from	stress	1658:1663	arg1	cells					1677:1681	high-fat cells	1668:1681	high-fat cells	1668:1681	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	5	38	theme	reaction	825:832	arg1	species					841:847	reaction oxygen species	825:847	reaction oxygen species (ROS) level	825:859	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	5	38	theme	reaction	825:832	arg1	ROS					850:852	ROS	850:852	ROS	850:852	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	0	39	theme	HepG2	121:125	arg1	Cells					127:131	HepG2 Cells	121:131	HepG2 Cells	121:131	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	0	40	from	Extract	88:94	arg1	Polysaccharides					45:59	Polysaccharides	45:59	Polysaccharides from Fermented Barley Bran Extract	45:94	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	0	40	from	Extract	88:94	arg1	Effect					35:40	the Regulatory Effect	20:40	the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells	20:131	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	3	41	theme	high-fat	539:546	arg1	cells					554:558	high-fat HepG2 cells	539:558	high-fat HepG2 cells induced by sodium oleate	539:583	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	5	42	theme	oxygen	834:839	arg1	species					841:847	reaction oxygen species	825:847	reaction oxygen species (ROS) level	825:859	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	5	42	theme	oxygen	834:839	arg1	ROS					850:852	ROS	850:852	ROS	850:852	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	3	43	theme	bran	391:394	arg1	extract					396:402	barley bran extract	384:402	barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS)	384:460	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	8	44	from	activities	1534:1543	arg1	bran					1574:1577	barley bran	1567:1577	barley bran	1567:1577	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	5	45	theme	species	841:847	arg1	level					855:859	reaction oxygen species (ROS) level	825:859	reaction oxygen species (ROS) level	825:859	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	3	46	theme	sodium	571:576	arg1	oleate					578:583	sodium oleate	571:583	sodium oleate	571:583	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	7	47	theme	scd-1	1436:1440	arg1	expression					1442:1451	scd-1 expression	1436:1451	scd-1 expression	1436:1451	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	1	48	theme	polyphenols	201:211	arg1	content					190:196	its high content	181:196	its high content of polyphenols and dietary fiber, etc	181:234	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	7	49	theme	expression	1442:1451	arg1	downregulation					1418:1431	the downregulation	1414:1431	the downregulation of scd-1 expression	1414:1451	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	5	50	theme	triglyceride	795:806	arg1	content					813:819	the triglyceride (TG) content	791:819	the triglyceride (TG) content	791:819	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	5	51	theme	high-fat	864:871	arg1	cells					879:883	high-fat HepG2 cells	864:883	high-fat HepG2 cells	864:883	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	5	52	theme	μg/mL	764:768	arg1	FBBE-PS					770:776	50 μg/mL FBBE-PS	761:776	50 μg/mL FBBE-PS	761:776	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	7	53	theme	unsaturated	1364:1374	arg1	acids					1382:1386	unsaturated fatty acids	1364:1386	unsaturated fatty acids	1364:1386	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	2	54	theme	food	328:331	arg1	materials					337:345	food raw materials	328:345	food raw materials	328:345	Fermentation has been considered as an effective way to promote the functional activity of food raw materials.
36837842	0	55	theme	Polysaccharides	45:59	arg1	Effect					35:40	the Regulatory Effect	20:40	the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells	20:131	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	8	56	from	bran	1574:1577	arg1	properties					1505:1514	properties	1505:1514	properties	1505:1514	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	56	from	bran	1574:1577	arg1	activities					1534:1543	physiological activities	1520:1543	physiological activities	1520:1543	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	57	theme	lipid	1627:1631	arg1	deposition					1633:1642	lipid deposition	1627:1642	lipid deposition	1627:1642	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	5	58	theme	catalase	987:994	arg1	activities					942:951	the activities	938:951	the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively	938:1047	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	0	59	from	Accumulation	105:116	arg1	Cells					127:131	HepG2 Cells	121:131	HepG2 Cells	121:131	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	7	60	theme	metabolomics	1252:1263	arg1	analysis					1265:1272	The metabolomics analysis	1248:1272	The metabolomics analysis	1248:1272	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36837842	4	61	theme	monosaccharide	660:673	arg1	composition					675:685	monosaccharide composition	660:685	monosaccharide composition of polysaccharides	660:704	The results showed that the molecular weight decreased and monosaccharide composition of polysaccharides changed significantly after fermentation.
36837842	6	62	theme	RT-qPCR	1050:1056	arg1	analysis					1058:1065	RT-qPCR analysis	1050:1065	RT-qPCR analysis	1050:1065	RT-qPCR analysis revealed that FBBE-PS could up-regulate the lipid metabolism-related genes such as ppar-α, acox-1 and cpt-1α, and oxidation-related genes such as nrf2, ho-1, nqo-1, sod1, cat, etc.
36837842	1	63	theme	dietary	217:223	arg1	etc					232:234	etc	232:234	etc	232:234	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	1	63	theme	dietary	217:223	arg1	fiber					225:229	dietary fiber	217:229	dietary fiber	217:229	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	3	64	theme	oxidative	519:527	arg1	stress					529:534	oxidative stress	519:534	oxidative stress	519:534	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	5	65	from	level	855:859	arg1	cells					879:883	high-fat HepG2 cells	864:883	high-fat HepG2 cells	864:883	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	2	66	theme	functional	305:314	arg1	activity					316:323	the functional activity	301:323	the functional activity of food raw materials	301:345	Fermentation has been considered as an effective way to promote the functional activity of food raw materials.
36837842	1	67	theme	fiber	225:229	arg1	content					190:196	its high content	181:196	its high content of polyphenols and dietary fiber, etc	181:234	Barley bran has potential bioactivities due to its high content of polyphenols and dietary fiber, etc.
36837842	0	68	theme	Barley	76:81	arg1	Extract					88:94	Fermented Barley Bran Extract	66:94	Fermented Barley Bran Extract	66:94	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	5	69	theme	dismutase	967:975	arg1	activities					942:951	the activities	938:951	the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively	938:1047	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	5	70	theme	HepG2	873:877	arg1	cells					879:883	high-fat HepG2 cells	864:883	high-fat HepG2 cells	864:883	In addition, 50 μg/mL FBBE-PS could reduce the triglyceride (TG) content and reaction oxygen species (ROS) level in high-fat HepG2 cells by 21.62% and 30.01%, respectively, while increasing the activities of superoxide dismutase (SOD) and catalase (CAT) represented by 64.87% and 22.93%, respectively.
36837842	4	71	theme	molecular	629:637	arg1	weight					639:644	the molecular weight	625:644	the molecular weight	625:644	The results showed that the molecular weight decreased and monosaccharide composition of polysaccharides changed significantly after fermentation.
36837842	8	72	theme	polysaccharides	1548:1562	arg1	properties					1505:1514	properties	1505:1514	properties	1505:1514	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	8	72	theme	polysaccharides	1548:1562	arg1	activities					1534:1543	physiological activities	1520:1543	physiological activities	1520:1543	It is demonstrated that fermentation can alter the properties and physiological activities of polysaccharides in barley bran, and FBBE-PS exhibited an alleviating effect on lipid deposition and oxidative stress in high-fat cells.
36837842	0	73	theme	Fermented	66:74	arg1	Extract					88:94	Fermented Barley Bran Extract	66:94	Fermented Barley Bran Extract	66:94	Metabolomics Reveal the Regulatory Effect of Polysaccharides from Fermented Barley Bran Extract on Lipid Accumulation in HepG2 Cells.
36837842	3	74	theme	lipid	496:500	arg1	accumulation					502:513	lipid accumulation	496:513	lipid accumulation	496:513	In this study, polysaccharides from barley bran extract fermented by Lactiplantibacillus plantarum dy-1 (FBBE-PS) were analyzed, and its effects on lipid accumulation and oxidative stress in high-fat HepG2 cells induced by sodium oleate were evaluated.
36837842	7	75	theme	lipid	1313:1317	arg1	deposition					1319:1328	lipid deposition	1313:1328	lipid deposition	1313:1328	The metabolomics analysis indicated that FBBE-PS could alleviate lipid deposition by inhibiting the biosynthesis of unsaturated fatty acids, which is consistent with the downregulation of scd-1 expression.
36339535	0	0	theme	acute	104:108	arg1	injury					117:122	cisplatin-induced acute kidney injury	86:122	cisplatin-induced acute kidney injury	86:122	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	5	1	theme	molecular	746:754	arg1	weight					756:761	molecular weight	746:761	molecular weight	746:761	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	7	2	theme	rat	1122:1124	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	6	3	theme	moderate	862:869	arg1	activity					883:890	moderate antioxidant activity	862:890	moderate antioxidant activity	862:890	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	4	4	theme	electron	675:682	arg1	SEM					696:698	SEM	696:698	SEM	696:698	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	4	4	theme	electron	675:682	arg1	microscopy					684:693	scanning electron microscopy	666:693	scanning electron microscopy (SEM) analysis	666:708	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	7	5	theme	cytoprotective	1044:1057	arg1	effects					1059:1065	cytoprotective effects	1044:1065	cytoprotective effects	1044:1065	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	0	6	theme	cisplatin-induced	86:102	arg1	injury					117:122	cisplatin-induced acute kidney injury	86:122	cisplatin-induced acute kidney injury	86:122	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	1	7	theme	lateral	340:346	arg1	polysaccharides					353:367	the lateral root polysaccharides	336:367	the lateral root polysaccharides of Aconitum carmichaelii	336:392	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	7	8	theme	renal	1126:1130	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	4	9	theme	scanning	666:673	arg1	SEM					696:698	SEM	696:698	SEM	696:698	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	4	9	theme	scanning	666:673	arg1	microscopy					684:693	scanning electron microscopy	666:693	scanning electron microscopy (SEM) analysis	666:708	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	1	10	theme	root	348:351	arg1	polysaccharides					353:367	the lateral root polysaccharides	336:367	the lateral root polysaccharides of Aconitum carmichaelii	336:392	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	6	11	theme	radical	1003:1009	arg1	rates					920:924	the scavenging rates	905:924	the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical	905:1029	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	0	12	theme	kidney	110:115	arg1	injury					117:122	cisplatin-induced acute kidney injury	86:122	cisplatin-induced acute kidney injury	86:122	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	1	13	theme	polysaccharides	353:367	arg1	parameters					322:331	the extraction parameters	307:331	the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii	307:392	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	6	14	theme	hydroxyl	994:1001	arg1	radical					1003:1009	hydroxyl radical	994:1009	hydroxyl radical	994:1009	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	6	15	theme	2,2-diphenyl-1-picrylhydrazyl	929:957	arg1	radical					959:965	2,2-diphenyl-1-picrylhydrazyl radical	929:965	2,2-diphenyl-1-picrylhydrazyl radical	929:965	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	9	16	from	Fuzi	1650:1653	arg1	polysaccharides					1629:1643	bioactive polysaccharides	1619:1643	bioactive polysaccharides from Fuzi	1619:1653	In conclusion, this study provides a good strategy for obtaining bioactive polysaccharides from Fuzi.
36339535	1	17	theme	one-way	265:271	arg1	experiments					273:283	one-way experiments	265:283	one-way experiments	265:283	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	18	used	used	290:293	arg2	BBD					251:253	BBD	251:253	BBD	251:253	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	18	used	used	290:293	arg2	RSM					221:223	RSM	221:223	RSM	221:223	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	18	used	used	290:293	arg2	design					243:248	Box- Behnken design	230:248	Box- Behnken design (BBD)	230:254	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	18	used	used	290:293	arg2	methodology					208:218	Response surface methodology	191:218	Response surface methodology (RSM)	191:224	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	7	19	theme	-induced	1099:1106	arg1	injury					1108:1113	hydrogen peroxide (H2O2)-induced injury	1075:1113	hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1075:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	1	20	theme	Response	191:198	arg1	methodology					208:218	Response surface methodology	191:218	Response surface methodology (RSM)	191:224	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	20	theme	Response	191:198	arg1	RSM					221:223	RSM	221:223	RSM	221:223	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	5	21	theme	monosaccharide	715:728	arg1	composition					730:740	monosaccharide composition	715:740	monosaccharide composition	715:740	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	6	22	theme	antioxidant	871:881	arg1	activity					883:890	moderate antioxidant activity	862:890	moderate antioxidant activity	862:890	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	7	23	theme	cell	1151:1154	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	8	24	theme	cisplatin-induced	1286:1302	arg1	injury					1317:1322	cisplatin-induced acute kidney injury	1286:1322	cisplatin-induced acute kidney injury in mice	1286:1330	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	3	25	theme	extraction	542:551	arg1	time					553:556	an extraction time	539:556	an extraction time of 2 h	539:563	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	1	26	theme	surface	200:206	arg1	methodology					208:218	Response surface methodology	191:218	Response surface methodology (RSM)	191:224	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	26	theme	surface	200:206	arg1	RSM					221:223	RSM	221:223	RSM	221:223	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	7	27	theme	tubular	1171:1177	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	1	28	theme	carmichaelii	381:392	arg1	polysaccharides					353:367	the lateral root polysaccharides	336:367	the lateral root polysaccharides of Aconitum carmichaelii	336:392	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	4	29	theme	infrared	635:642	arg1	spectroscopy					644:655	infrared spectroscopy	635:655	infrared spectroscopy (IR)	635:660	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	4	29	theme	infrared	635:642	arg1	IR					658:659	IR	658:659	IR	658:659	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	3	30	dep	ratio	526:530	arg1	to					510:511	to	510:511	to	510:511	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	5	31	theme	high-performance	788:803	arg1	HPLC					828:831	HPLC	828:831	HPLC	828:831	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	5	31	theme	high-performance	788:803	arg1	chromatography					812:825	high-performance liquid chromatography	788:825	high-performance liquid chromatography (HPLC)	788:832	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	7	32	theme	line	1156:1159	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	8	33	theme	4-hydroxynonenal	1466:1481	arg1	levels					1430:1435	the levels	1426:1435	the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE)	1426:1489	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	8	34	theme	glutathione	1359:1369	arg1	levels					1349:1354	the levels	1345:1354	the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4)	1345:1412	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	9	35	theme	bioactive	1619:1627	arg1	polysaccharides					1629:1643	bioactive polysaccharides	1619:1643	bioactive polysaccharides from Fuzi	1619:1653	In conclusion, this study provides a good strategy for obtaining bioactive polysaccharides from Fuzi.
36339535	5	36	theme	liquid	805:810	arg1	HPLC					828:831	HPLC	828:831	HPLC	828:831	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	5	36	theme	liquid	805:810	arg1	chromatography					812:825	high-performance liquid chromatography	788:825	high-performance liquid chromatography (HPLC)	788:832	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	6	37	theme	radical	985:991	arg1	rates					920:924	the scavenging rates	905:924	the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical	905:1029	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	8	38	theme	kidney	1310:1315	arg1	injury					1317:1322	cisplatin-induced acute kidney injury	1286:1322	cisplatin-induced acute kidney injury in mice	1286:1330	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	8	39	from	injury	1317:1322	arg1	mice					1327:1330	mice	1327:1330	mice	1327:1330	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	3	40	theme	2 h	561:563	arg1	time					553:556	an extraction time	539:556	an extraction time of 2 h	539:563	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	3	40	theme	2 h	561:563	arg1	temperature					584:594	an extraction temperature	570:594	an extraction temperature of 90°C	570:602	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	3	40	theme	2 h	561:563	arg1	ratio					526:530	a water to raw material ratio	502:530	a water to raw material ratio of 43	502:536	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	8	41	theme	glutathione	1381:1391	arg1	levels					1349:1354	the levels	1345:1354	the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4)	1345:1412	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	2	42	theme	refined	439:445	arg1	polysaccharides					409:423	The extracted polysaccharides	395:423	The extracted polysaccharides	395:423	The extracted polysaccharides were named as refined fucose polysaccharide.
36339535	2	42	theme	refined	439:445	arg1	polysaccharide					454:467	refined fucose polysaccharide	439:467	refined fucose polysaccharide	439:467	The extracted polysaccharides were named as refined fucose polysaccharide.
36339535	2	43	theme	fucose	447:452	arg1	polysaccharides					409:423	The extracted polysaccharides	395:423	The extracted polysaccharides	395:423	The extracted polysaccharides were named as refined fucose polysaccharide.
36339535	2	43	theme	fucose	447:452	arg1	polysaccharide					454:467	refined fucose polysaccharide	439:467	refined fucose polysaccharide	439:467	The extracted polysaccharides were named as refined fucose polysaccharide.
36339535	6	44	theme	anion	979:983	arg1	radical					985:991	superoxide anion radical	968:991	superoxide anion radical	968:991	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	6	45	theme	scavenging	909:918	arg1	rates					920:924	the scavenging rates	905:924	the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical	905:1029	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	4	46	theme	RFP	618:620	arg1	shape					609:613	The shape	605:613	The shape of RFP	605:620	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	0	47	theme	antioxidant	42:52	arg1	activity					54:61	antioxidant activity	42:61	antioxidant activity	42:61	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	4	48	theme	microscopy	684:693	arg1	analysis					701:708	scanning electron microscopy (SEM) analysis	666:708	scanning electron microscopy (SEM) analysis	666:708	The shape of RFP was shown by infrared spectroscopy (IR) and scanning electron microscopy (SEM) analysis.
36339535	5	49	dep	composition	730:740	arg1	The					711:713	The	711:713	The	711:713	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	7	50	theme	epithelial	1179:1188	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	6	51	theme	superoxide	968:977	arg1	radical					985:991	superoxide anion radical	968:991	superoxide anion radical	968:991	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	7	52	dep	exerted	1036:1042	arg1	NRK-52E					1197:1203	NRK-52E	1197:1203	NRK-52E	1197:1203	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	3	53	theme	optimal	474:480	arg1	conditions					482:491	The optimal conditions	470:491	The optimal conditions	470:491	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	5	54	theme	RFP	766:768	arg1	composition					730:740	monosaccharide composition	715:740	monosaccharide composition	715:740	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	5	54	theme	RFP	766:768	arg1	weight					756:761	molecular weight	746:761	molecular weight	746:761	The monosaccharide composition and molecular weight of RFP was determined by high-performance liquid chromatography (HPLC).
36339535	0	55	theme	lateral	152:158	arg1	root					160:163	the lateral root	148:163	the lateral root of Aconitum carmichaelii	148:188	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	8	56	theme	malondialdehyde	1440:1454	arg1	levels					1430:1435	the levels	1426:1435	the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE)	1426:1489	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	3	57	theme	90°C	599:602	arg1	time					553:556	an extraction time	539:556	an extraction time of 2 h	539:563	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	3	57	theme	90°C	599:602	arg1	temperature					584:594	an extraction temperature	570:594	an extraction temperature of 90°C	570:602	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	3	57	theme	90°C	599:602	arg1	ratio					526:530	a water to raw material ratio	502:530	a water to raw material ratio of 43	502:536	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	1	58	theme	Box-	230:233	arg1	BBD					251:253	BBD	251:253	BBD	251:253	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	58	theme	Box-	230:233	arg1	design					243:248	Box- Behnken design	230:248	Box- Behnken design (BBD)	230:254	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	7	59	theme	tubular	1132:1138	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	6	60	theme	radical	959:965	arg1	rates					920:924	the scavenging rates	905:924	the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical	905:1029	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	0	61	theme	Aconitum	168:175	arg1	carmichaelii					177:188	Aconitum carmichaelii	168:188	Aconitum carmichaelii	168:188	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	3	62	theme	extraction	573:582	arg1	temperature					584:594	an extraction temperature	570:594	an extraction temperature of 90°C	570:602	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	3	63	theme	raw	513:515	arg1	ratio					526:530	a water to raw material ratio	502:530	a water to raw material ratio of 43	502:536	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	7	64	theme	hydrogen	1075:1082	arg1	H2O2					1094:1097	H2O2	1094:1097	H2O2	1094:1097	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	7	64	theme	hydrogen	1075:1082	arg1	peroxide					1084:1091	hydrogen peroxide	1075:1091	hydrogen peroxide (H2O2)	1075:1098	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	1	65	theme	extraction	311:320	arg1	parameters					322:331	the extraction parameters	307:331	the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii	307:392	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	7	66	theme	renal	1165:1169	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	3	67	theme	material	517:524	arg1	ratio					526:530	a water to raw material ratio	502:530	a water to raw material ratio of 43	502:536	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	7	68	theme	rat	1161:1163	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	7	69	theme	epithelial	1140:1149	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	3	70	theme	water	504:508	arg1	ratio					526:530	a water to raw material ratio	502:530	a water to raw material ratio of 43	502:536	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	0	71	from	root	160:163	arg1	polysaccharides					127:141	polysaccharides	127:141	polysaccharides from the lateral root of Aconitum carmichaelii	127:188	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	0	72	theme	carmichaelii	177:188	arg1	root					160:163	the lateral root	148:163	the lateral root of Aconitum carmichaelii	148:188	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	9	73	theme	good	1591:1594	arg1	strategy					1596:1603	a good strategy	1589:1603	a good strategy for obtaining bioactive polysaccharides from Fuzi	1589:1653	In conclusion, this study provides a good strategy for obtaining bioactive polysaccharides from Fuzi.
36339535	7	74	from	injury	1108:1113	arg1	cells					1190:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	the rat renal tubular epithelial cell line rat renal tubular epithelial cells	1118:1194	RFP exerted cytoprotective effects against hydrogen peroxide (H2O2)-induced injury in the rat renal tubular epithelial cell line rat renal tubular epithelial cells (NRK-52E) and inhibited apoptosis.
36339535	8	75	theme	acute	1304:1308	arg1	injury					1317:1322	cisplatin-induced acute kidney injury	1286:1322	cisplatin-induced acute kidney injury in mice	1286:1330	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	6	76	theme	radical	1023:1029	arg1	rates					920:924	the scavenging rates	905:924	the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical	905:1029	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36339535	8	77	theme	lipid	1501:1505	arg1	peroxidation					1507:1518	lipid peroxidation	1501:1518	lipid peroxidation	1501:1518	In addition, researches found that RFP could alleviate cisplatin-induced acute kidney injury in mice by enhancing the levels of glutathione (GSH) and glutathione peroxidase-4 (GPX-4), decreasing the levels of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), reducing lipid peroxidation, and thus inhibiting ferroptosis.
36339535	3	78	theme	43	535:536	arg1	time					553:556	an extraction time	539:556	an extraction time of 2 h	539:563	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	3	78	theme	43	535:536	arg1	temperature					584:594	an extraction temperature	570:594	an extraction temperature of 90°C	570:602	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	3	78	theme	43	535:536	arg1	ratio					526:530	a water to raw material ratio	502:530	a water to raw material ratio of 43	502:536	The optimal conditions included a water to raw material ratio of 43, an extraction time of 2 h, and an extraction temperature of 90°C.
36339535	2	79	theme	extracted	399:407	arg1	polysaccharides					409:423	The extracted polysaccharides	395:423	The extracted polysaccharides	395:423	The extracted polysaccharides were named as refined fucose polysaccharide.
36339535	2	79	theme	extracted	399:407	arg1	polysaccharide					454:467	refined fucose polysaccharide	439:467	refined fucose polysaccharide	439:467	The extracted polysaccharides were named as refined fucose polysaccharide.
36339535	0	80	theme	structural	13:22	arg1	characterization					24:39	structural characterization	13:39	structural characterization	13:39	Preparation, structural characterization, antioxidant activity and protection against cisplatin-induced acute kidney injury by polysaccharides from the lateral root of Aconitum carmichaelii.
36339535	1	81	theme	Behnken	235:241	arg1	BBD					251:253	BBD	251:253	BBD	251:253	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	1	81	theme	Behnken	235:241	arg1	design					243:248	Box- Behnken design	230:248	Box- Behnken design (BBD)	230:254	Response surface methodology (RSM) and Box- Behnken design (BBD) based on one-way experiments were used to optimize the extraction parameters of the lateral root polysaccharides of Aconitum carmichaelii.
36339535	6	82	theme	ABTS	1016:1019	arg1	rates					920:924	the scavenging rates	905:924	the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical	905:1029	Furthermore, RFP exhibited moderate antioxidant activity by analyzing the scavenging rates of 2,2-diphenyl-1-picrylhydrazyl radical, superoxide anion radical, hydroxyl radical, and ABTS + radical.
36836701	9	0	theme	experiment	1517:1526	arg1	end					1498:1500	the end	1494:1500	the end of the feeding experiment	1494:1526	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	1	theme	growth-related	1609:1622	arg1	genes					1624:1628	growth-related genes	1609:1628	growth-related genes	1609:1628	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	1	2	theme	dietary	235:241	arg1	supplementation					243:257	dietary supplementation	235:257	dietary supplementation	235:257	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	7	3	theme	heterotrophic	1345:1357	arg1	bacteria					1359:1366	heterotrophic bacteria	1345:1366	heterotrophic bacteria	1345:1366	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	10	4	theme	survival	2062:2069	arg1	rate					2071:2074	survival rate	2062:2074	survival rate	2062:2074	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	7	5	theme	microbial	1305:1313	arg1	abundance					1315:1323	the microbial abundance	1301:1323	the microbial abundance	1301:1323	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	9	6	theme	stress	1793:1798	arg1	genes					1800:1804	stress genes	1793:1804	stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx)	1793:1865	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	6	theme	stress	1793:1798	arg1	dismutase					1818:1826	Superoxide dismutase	1807:1826	Superoxide dismutase (SOD)	1807:1832	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	6	theme	stress	1793:1798	arg1	peroxidase					1850:1859	Glutathione peroxidase	1838:1859	Glutathione peroxidase (GPx)	1838:1865	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	1	7	theme	genes	457:461	arg1	abundance					403:411	microbial abundance	393:411	microbial abundance	393:411	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	7	theme	genes	457:461	arg1	utilization					354:364	feed utilization	349:364	feed utilization	349:364	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	7	theme	genes	457:461	arg1	expressions					414:424	expressions	414:424	expressions of growth and immunity-related genes	414:461	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	7	theme	genes	457:461	arg1	indices					340:346	growth indices	333:346	growth indices	333:346	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	7	theme	genes	457:461	arg1	compositions					379:390	biochemical compositions	367:390	biochemical compositions	367:390	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	7	theme	genes	457:461	arg1	genes					475:479	stress genes	468:479	stress genes of the Pacific Whiteleg shrimp	468:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	9	8	dep	genes	1800:1804	arg1	SOD					1829:1831	SOD	1829:1831	SOD	1829:1831	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	8	dep	genes	1800:1804	arg1	dismutase					1818:1826	Superoxide dismutase	1807:1826	Superoxide dismutase (SOD)	1807:1832	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	8	dep	genes	1800:1804	arg1	genes					1800:1804	stress genes	1793:1804	stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx)	1793:1865	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	8	dep	genes	1800:1804	arg1	peroxidase					1850:1859	Glutathione peroxidase	1838:1859	Glutathione peroxidase (GPx)	1838:1865	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	8	dep	genes	1800:1804	arg1	GPx					1862:1864	GPx	1862:1864	GPx	1862:1864	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	2	9	theme	±	711:711	arg1	weight					693:698	an initial weight	682:698	an initial weight of (0.0017 ± 0.001 g)	682:720	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	2	9	theme	±	711:711	arg1	g					719:719	0.0017 ± 0.001 g	704:719	0.0017 ± 0.001 g	704:719	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	4	10	theme	SWP	935:937	arg1	levels					940:945	different seaweed polysaccharide (SWP) levels	901:945	different seaweed polysaccharide (SWP) levels	901:945	Three experimental diets were prepared with different seaweed polysaccharide (SWP) levels.
36836701	2	11	dep	aquarium	618:625	arg1	L					631:631	40 L	628:631	40 L of each	628:639	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	9	12	theme	polysaccharide	1560:1573	arg1	levels					1575:1580	polysaccharide levels	1560:1580	polysaccharide levels	1560:1580	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	7	13	theme	biochemical	1273:1283	arg1	composition					1285:1295	Whole-body biochemical composition	1262:1295	Whole-body biochemical composition	1262:1295	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	1	14	from	impact	225:230	arg1	abundance					403:411	microbial abundance	393:411	microbial abundance	393:411	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	14	from	impact	225:230	arg1	utilization					354:364	feed utilization	349:364	feed utilization	349:364	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	14	from	impact	225:230	arg1	expressions					414:424	expressions	414:424	expressions of growth and immunity-related genes	414:461	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	14	from	impact	225:230	arg1	indices					340:346	growth indices	333:346	growth indices	333:346	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	14	from	impact	225:230	arg1	compositions					379:390	biochemical compositions	367:390	biochemical compositions	367:390	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	14	from	impact	225:230	arg1	genes					475:479	stress genes	468:479	stress genes of the Pacific Whiteleg shrimp	468:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	10	15	theme	vannamei	2257:2264	arg1	immunity-					2205:2213	immunity-	2205:2213	immunity-	2205:2213	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	10	15	theme	vannamei	2257:2264	arg1	growth-					2196:2202	the growth-	2192:2202	the growth-	2192:2202	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	10	15	theme	vannamei	2257:2264	arg1	expressions					2239:2249	stress-related gene expressions	2219:2249	stress-related gene expressions	2219:2249	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	2	16	theme	post-larvae	549:559	arg1	total					536:540	A total	534:540	A total of 360 post-larvae of L. vannamei	534:574	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	0	17	theme	Immune-Related	106:119	arg1	Expression					127:136	Immune-Related Genes Expression	106:136	Immune-Related Genes Expression of The Pacific Whiteleg Shrimp	106:167	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	10	18	theme	incorporation	2102:2114	arg1	level					2116:2120	the incorporation level	2098:2120	the incorporation level of 3 g kg-1	2098:2132	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	1	19	theme	microbial	393:401	arg1	abundance					403:411	microbial abundance	393:411	microbial abundance	393:411	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	5	20	contain	contained	1034:1042	arg1	SWP1					1013:1016	SWP1	1013:1016	SWP1	1013:1016	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	20	contain	contained	1034:1042	arg1	SWP2					1019:1022	SWP2	1019:1022	SWP2	1019:1022	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	20	contain	contained	1034:1042	arg2	SWP1					1013:1016	SWP1	1013:1016	SWP1	1013:1016	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	20	contain	contained	1034:1042	arg1	concentrations					1063:1076	concentrations	1063:1076	concentrations of 1, 2, and 3 g kg-1 diet	1063:1103	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	20	contain	contained	1034:1042	arg2	SWP3					1029:1032	SWP3	1029:1032	SWP3	1029:1032	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	20	contain	contained	1034:1042	arg2	SWP2					1019:1022	SWP2	1019:1022	SWP2	1019:1022	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	20	contain	contained	1034:1042	arg2	polysaccharides					1044:1058	polysaccharides	1044:1058	polysaccharides	1044:1058	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	20	contain	contained	1034:1042	arg1	SWP3					1029:1032	SWP3	1029:1032	SWP3	1029:1032	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	2	21	theme	shrimp	670:675	arg1	density					656:662	a stocking density	645:662	a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g)	645:720	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	6	22	theme	significant	1173:1183	arg1	improvements					1185:1196	significant improvements	1173:1196	significant improvements in weight gain and survival rate	1173:1229	Diets supplemented with polysaccharide levels showed significant improvements in weight gain and survival rate, compared to the control diet.
36836701	10	23	theme	g	2127:2127	arg1	kg-1					2129:2132	3 g kg-1	2125:2132	3 g kg-1	2125:2132	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	3	24	theme	experiment	741:750	arg1	trial					752:756	the 90-day experiment trial	730:756	the 90-day experiment trial	730:756	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	0	25	theme	Whiteleg	153:160	arg1	Shrimp					162:167	The Pacific Whiteleg Shrimp	141:167	The Pacific Whiteleg Shrimp	141:167	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	5	26	theme	control	958:964	arg1	diet					966:969	The basal control diet	948:969	The basal control diet	948:969	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	10	27	theme	weight	2046:2051	arg1	gain					2053:2056	weight gain	2046:2056	weight gain	2046:2056	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	9	28	theme	growth	1644:1649	arg1	IGF-I					1660:1664	IGF-I	1660:1664	IGF-I	1660:1664	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	28	theme	growth	1644:1649	arg1	factors					1651:1657	Insulin-like growth factors	1631:1657	Insulin-like growth factors (IGF-I, IGF-II)	1631:1673	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	10	29	theme	polysaccharide	1980:1993	arg1	rate					1960:1963	the inclusion rate	1946:1963	the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration	1946:2030	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	6	30	theme	control	1248:1254	arg1	diet					1256:1259	the control diet	1244:1259	the control diet	1244:1259	Diets supplemented with polysaccharide levels showed significant improvements in weight gain and survival rate, compared to the control diet.
36836701	5	31	contain	had	971:973	arg2	level					993:997	no polysaccharide level	975:997	no polysaccharide level (SWP0)	975:1004	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	31	contain	had	971:973	arg1	diet					966:969	The basal control diet	948:969	The basal control diet	948:969	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	31	contain	had	971:973	arg2	SWP0					1000:1003	SWP0	1000:1003	SWP0	1000:1003	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	3	32	dep	times	844:848	arg1	day					852:854	a day	850:854	three times a day	838:854	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	9	33	dep	genes	1691:1695	arg1	Crustin					1779:1785	Crustin	1779:1785	Crustin	1779:1785	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	33	dep	genes	1691:1695	arg1	Prophenoloxidase					1733:1748	Prophenoloxidase	1733:1748	Prophenoloxidase (ProPO)	1733:1756	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	33	dep	genes	1691:1695	arg1	Lys					1769:1771	Lys	1769:1771	Lys	1769:1771	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	33	dep	genes	1691:1695	arg1	protein					1716:1722	β -Glucan-binding protein	1698:1722	β -Glucan-binding protein (β-Bgp)	1698:1730	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	33	dep	genes	1691:1695	arg1	ProPO					1751:1755	ProPO	1751:1755	ProPO	1751:1755	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	33	dep	genes	1691:1695	arg1	β-Bgp					1725:1729	β-Bgp	1725:1729	β-Bgp	1725:1729	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	33	dep	genes	1691:1695	arg1	genes					1691:1695	immune-related genes	1676:1695	immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin)	1676:1786	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	33	dep	genes	1691:1695	arg1	Lysozyme					1759:1766	Lysozyme	1759:1766	Lysozyme (Lys)	1759:1772	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	10	34	theme	kg-1	1972:1975	arg1	rate					1960:1963	the inclusion rate	1946:1963	the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration	1946:2030	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	2	35	from	density	656:662	arg1	aquarium					618:625	a 12-glass aquarium	607:625	a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g)	607:720	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	3	36	theme	shrimp	763:768	arg1	larvae					770:775	all shrimp larvae	759:775	all shrimp larvae	759:775	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	0	37	theme	Microbial	70:78	arg1	Abundance					80:88	Microbial Abundance	70:88	Microbial Abundance	70:88	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	7	38	dep	composition	1285:1295	arg1	count					1336:1340	the total count	1326:1340	the total count of heterotrophic bacteria	1326:1366	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	7	38	dep	composition	1285:1295	arg1	spp					1379:1381	Vibrio spp	1372:1381	Vibrio spp	1372:1381	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	0	39	from	Impact	0:5	arg1	Growth					62:67	Growth	62:67	Growth	62:67	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	0	39	from	Impact	0:5	arg1	Abundance					80:88	Microbial Abundance	70:88	Microbial Abundance	70:88	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	0	39	from	Impact	0:5	arg1	Growth					95:100	Growth	95:100	Growth	95:100	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	2	40	theme	each	636:639	arg1	L					631:631	40 L	628:631	40 L of each	628:639	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	1	41	theme	feed	349:352	arg1	utilization					354:364	feed utilization	349:364	feed utilization	349:364	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	0	42	dep	Growth	95:100	arg1	vannamei					182:189	Litopenaeus vannamei	170:189	Litopenaeus vannamei	170:189	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	3	43	theme	body	825:828	arg1	weight					830:835	total body weight	819:835	total body weight	819:835	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	3	43	theme	body	825:828	arg1	times					844:848	three times a day	838:854	three times a day	838:854	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	4	44	theme	experimental	863:874	arg1	diets					876:880	Three experimental diets	857:880	Three experimental diets	857:880	Three experimental diets were prepared with different seaweed polysaccharide (SWP) levels.
36836701	6	45	theme	survival	1217:1224	arg1	rate					1226:1229	survival rate	1217:1229	survival rate	1217:1229	Diets supplemented with polysaccharide levels showed significant improvements in weight gain and survival rate, compared to the control diet.
36836701	9	46	theme	-Glucan-binding	1700:1714	arg1	protein					1716:1722	β -Glucan-binding protein	1698:1722	β -Glucan-binding protein (β-Bgp)	1698:1730	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	46	theme	-Glucan-binding	1700:1714	arg1	genes					1691:1695	immune-related genes	1676:1695	immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin)	1676:1786	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	46	theme	-Glucan-binding	1700:1714	arg1	β-Bgp					1725:1729	β-Bgp	1725:1729	β-Bgp	1725:1729	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	47	theme	immune-related	1676:1689	arg1	Prophenoloxidase					1733:1748	Prophenoloxidase	1733:1748	Prophenoloxidase (ProPO)	1733:1756	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	47	theme	immune-related	1676:1689	arg1	protein					1716:1722	β -Glucan-binding protein	1698:1722	β -Glucan-binding protein (β-Bgp)	1698:1730	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	47	theme	immune-related	1676:1689	arg1	Lysozyme					1759:1766	Lysozyme	1759:1766	Lysozyme (Lys)	1759:1772	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	47	theme	immune-related	1676:1689	arg1	genes					1691:1695	immune-related genes	1676:1695	immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin)	1676:1786	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	47	theme	immune-related	1676:1689	arg1	Crustin					1779:1785	Crustin	1779:1785	Crustin	1779:1785	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	2	48	theme	stocking	647:654	arg1	density					656:662	a stocking density	645:662	a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g)	645:720	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	1	49	theme	polysaccharide	262:275	arg1	supplementation					243:257	dietary supplementation	235:257	dietary supplementation	235:257	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	8	50	theme	significant	1407:1417	arg1	differences					1419:1429	significant differences	1407:1429	significant differences among polysaccharide-treated diets	1407:1464	of L. vannamei showed significant differences among polysaccharide-treated diets compared to the control.
36836701	0	51	theme	Dietary	10:16	arg1	Administration					18:31	Dietary Administration	10:31	Dietary Administration of Seaweed Polysaccharide	10:57	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	4	52	theme	seaweed	911:917	arg1	levels					940:945	different seaweed polysaccharide (SWP) levels	901:945	different seaweed polysaccharide (SWP) levels	901:945	Three experimental diets were prepared with different seaweed polysaccharide (SWP) levels.
36836701	10	53	theme	current	1917:1923	arg1	study					1925:1929	the current study	1913:1929	the current study	1913:1929	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	9	54	theme	muscle	1874:1879	arg1	tissue					1881:1886	the muscle tissue	1870:1886	the muscle tissue of L. vannamei	1870:1901	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	1	55	theme	brown	293:297	arg1	seaweeds					299:306	brown seaweeds	293:306	brown seaweeds	293:306	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	10	56	theme	gene	2234:2237	arg1	expressions					2239:2249	stress-related gene expressions	2219:2249	stress-related gene expressions	2219:2249	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	1	57	theme	Pacific	488:494	arg1	shrimp					505:510	the Pacific Whiteleg shrimp	484:510	the Pacific Whiteleg shrimp	484:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	0	58	theme	Polysaccharide	44:57	arg1	Administration					18:31	Dietary Administration	10:31	Dietary Administration of Seaweed Polysaccharide	10:57	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	6	59	from	improvements	1185:1196	arg1	gain					1208:1211	weight gain	1201:1211	weight gain	1201:1211	Diets supplemented with polysaccharide levels showed significant improvements in weight gain and survival rate, compared to the control diet.
36836701	6	59	from	improvements	1185:1196	arg1	rate					1226:1229	survival rate	1217:1229	survival rate	1217:1229	Diets supplemented with polysaccharide levels showed significant improvements in weight gain and survival rate, compared to the control diet.
36836701	9	60	theme	vannamei	1894:1901	arg1	tissue					1881:1886	the muscle tissue	1870:1886	the muscle tissue of L. vannamei	1870:1901	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	61	theme	genes	1624:1628	arg1	expression					1595:1604	the expression	1591:1604	the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei	1591:1901	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	1	62	theme	shrimp	505:510	arg1	abundance					403:411	microbial abundance	393:411	microbial abundance	393:411	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	62	theme	shrimp	505:510	arg1	utilization					354:364	feed utilization	349:364	feed utilization	349:364	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	62	theme	shrimp	505:510	arg1	expressions					414:424	expressions	414:424	expressions of growth and immunity-related genes	414:461	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	62	theme	shrimp	505:510	arg1	indices					340:346	growth indices	333:346	growth indices	333:346	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	62	theme	shrimp	505:510	arg1	compositions					379:390	biochemical compositions	367:390	biochemical compositions	367:390	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	62	theme	shrimp	505:510	arg1	genes					475:479	stress genes	468:479	stress genes of the Pacific Whiteleg shrimp	468:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	7	63	theme	Vibrio	1372:1377	arg1	spp					1379:1381	Vibrio spp	1372:1381	Vibrio spp	1372:1381	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	5	64	theme	g	1093:1093	arg1	diet					1100:1103	1, 2, and 3 g kg-1 diet	1081:1103	1, 2, and 3 g kg-1 diet	1081:1103	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	7	65	theme	bacteria	1359:1366	arg1	count					1336:1340	the total count	1326:1340	the total count of heterotrophic bacteria	1326:1366	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	7	65	theme	bacteria	1359:1366	arg1	spp					1379:1381	Vibrio spp	1372:1381	Vibrio spp	1372:1381	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	9	66	theme	feeding	1509:1515	arg1	experiment					1517:1526	the feeding experiment	1505:1526	the feeding experiment	1505:1526	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	6	67	theme	polysaccharide	1144:1157	arg1	levels					1159:1164	polysaccharide levels	1144:1164	polysaccharide levels	1144:1164	Diets supplemented with polysaccharide levels showed significant improvements in weight gain and survival rate, compared to the control diet.
36836701	10	68	theme	vannamei	2082:2089	arg1	rate					2071:2074	survival rate	2062:2074	survival rate	2062:2074	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	10	68	theme	vannamei	2082:2089	arg1	gain					2053:2056	weight gain	2046:2056	weight gain	2046:2056	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	1	69	theme	immunity-related	440:455	arg1	genes					457:461	immunity-related genes	440:461	immunity-related genes	440:461	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	70	theme	supplementation	243:257	arg1	impact					225:230	the impact	221:230	the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp	221:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	7	71	theme	total	1330:1334	arg1	count					1336:1340	the total count	1326:1340	the total count of heterotrophic bacteria	1326:1366	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	9	72	theme	dietary	1533:1539	arg1	supplementation					1541:1555	the dietary supplementation	1529:1555	the dietary supplementation of polysaccharide levels	1529:1580	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	10	73	theme	stress-related	2219:2232	arg1	expressions					2239:2249	stress-related gene expressions	2219:2249	stress-related gene expressions	2219:2249	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	1	74	theme	stress	468:473	arg1	genes					475:479	stress genes	468:479	stress genes of the Pacific Whiteleg shrimp	468:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	2	75	theme	12-glass	609:616	arg1	aquarium					618:625	a 12-glass aquarium	607:625	a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g)	607:720	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	10	76	theme	inclusion	1950:1958	arg1	rate					1960:1963	the inclusion rate	1946:1963	the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration	1946:2030	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	0	77	theme	Genes	121:125	arg1	Expression					127:136	Immune-Related Genes Expression	106:136	Immune-Related Genes Expression of The Pacific Whiteleg Shrimp	106:167	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	7	78	theme	Whole-body	1262:1271	arg1	composition					1285:1295	Whole-body biochemical composition	1262:1295	Whole-body biochemical composition	1262:1295	Whole-body biochemical composition and the microbial abundance (the total count of heterotrophic bacteria and Vibrio spp.)
36836701	6	79	theme	weight	1201:1206	arg1	gain					1208:1211	weight gain	1201:1211	weight gain	1201:1211	Diets supplemented with polysaccharide levels showed significant improvements in weight gain and survival rate, compared to the control diet.
36836701	9	80	theme	levels	1575:1580	arg1	supplementation					1541:1555	the dietary supplementation	1529:1555	the dietary supplementation of polysaccharide levels	1529:1580	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	10	81	theme	g	1970:1970	arg1	kg-1					1972:1975	2 g kg-1	1968:1975	2 g kg-1	1968:1975	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	10	82	theme	pathogenic	2159:2168	arg1	microbes					2170:2177	pathogenic microbes	2159:2177	pathogenic microbes	2159:2177	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	0	83	theme	Pacific	145:151	arg1	Shrimp					162:167	The Pacific Whiteleg Shrimp	141:167	The Pacific Whiteleg Shrimp	141:167	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	2	84	theme	vannamei	567:574	arg1	post-larvae					549:559	360 post-larvae	545:559	360 post-larvae of L. vannamei	545:574	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	9	85	theme	genes	1800:1804	arg1	expression					1595:1604	the expression	1591:1604	the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei	1591:1901	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	0	86	theme	Shrimp	162:167	arg1	Expression					127:136	Immune-Related Genes Expression	106:136	Immune-Related Genes Expression of The Pacific Whiteleg Shrimp	106:167	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	0	86	theme	Shrimp	162:167	arg1	Growth					62:67	Growth	62:67	Growth	62:67	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	0	86	theme	Shrimp	162:167	arg1	Abundance					80:88	Microbial Abundance	70:88	Microbial Abundance	70:88	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	0	86	theme	Shrimp	162:167	arg1	Growth					95:100	Growth	95:100	Growth	95:100	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	5	87	theme	basal	952:956	arg1	diet					966:969	The basal control diet	948:969	The basal control diet	948:969	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	10	88	theme	additive	2008:2015	arg1	administration					2017:2030	a dietary additive administration	1998:2030	a dietary additive administration	1998:2030	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	3	89	theme	respective	792:801	arg1	diets					803:807	their respective diets	786:807	their respective diets	786:807	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	9	90	theme	Insulin-like	1631:1642	arg1	IGF-I					1660:1664	IGF-I	1660:1664	IGF-I	1660:1664	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	90	theme	Insulin-like	1631:1642	arg1	factors					1651:1657	Insulin-like growth factors	1631:1657	Insulin-like growth factors (IGF-I, IGF-II)	1631:1673	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	91	theme	Superoxide	1807:1816	arg1	genes					1800:1804	stress genes	1793:1804	stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx)	1793:1865	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	91	theme	Superoxide	1807:1816	arg1	dismutase					1818:1826	Superoxide dismutase	1807:1826	Superoxide dismutase (SOD)	1807:1832	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	91	theme	Superoxide	1807:1816	arg1	SOD					1829:1831	SOD	1829:1831	SOD	1829:1831	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	1	92	theme	growth	429:434	arg1	abundance					403:411	microbial abundance	393:411	microbial abundance	393:411	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	92	theme	growth	429:434	arg1	utilization					354:364	feed utilization	349:364	feed utilization	349:364	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	92	theme	growth	429:434	arg1	expressions					414:424	expressions	414:424	expressions of growth and immunity-related genes	414:461	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	92	theme	growth	429:434	arg1	indices					340:346	growth indices	333:346	growth indices	333:346	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	92	theme	growth	429:434	arg1	compositions					379:390	biochemical compositions	367:390	biochemical compositions	367:390	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	1	92	theme	growth	429:434	arg1	genes					475:479	stress genes	468:479	stress genes of the Pacific Whiteleg shrimp	468:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	9	93	theme	factors	1651:1657	arg1	expression					1595:1604	the expression	1591:1604	the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei	1591:1901	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	2	94	theme	initial	685:691	arg1	weight					693:698	an initial weight	682:698	an initial weight of (0.0017 ± 0.001 g)	682:720	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	2	94	theme	initial	685:691	arg1	g					719:719	0.0017 ± 0.001 g	704:719	0.0017 ± 0.001 g	704:719	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	5	95	theme	diet	1100:1103	arg1	concentrations					1063:1076	concentrations	1063:1076	concentrations of 1, 2, and 3 g kg-1 diet	1063:1103	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	3	96	theme	90-day	734:739	arg1	trial					752:756	the 90-day experiment trial	730:756	the 90-day experiment trial	730:756	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	1	97	theme	growth	333:338	arg1	indices					340:346	growth indices	333:346	growth indices	333:346	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	9	98	from	expression	1595:1604	arg1	tissue					1881:1886	the muscle tissue	1870:1886	the muscle tissue of L. vannamei	1870:1901	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	99	dep	IGF-I	1660:1664	arg1	IGF-II					1667:1672	IGF-II	1667:1672	IGF-II	1667:1672	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	10	100	theme	dietary	2000:2006	arg1	administration					2017:2030	a dietary additive administration	1998:2030	a dietary additive administration	1998:2030	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	9	101	theme	Glutathione	1838:1848	arg1	genes					1800:1804	stress genes	1793:1804	stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx)	1793:1865	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	101	theme	Glutathione	1838:1848	arg1	GPx					1862:1864	GPx	1862:1864	GPx	1862:1864	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	101	theme	Glutathione	1838:1848	arg1	peroxidase					1850:1859	Glutathione peroxidase	1838:1859	Glutathione peroxidase (GPx)	1838:1865	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	3	102	theme	weight	830:835	arg1	weight					830:835	total body weight	819:835	total body weight	819:835	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	3	102	theme	weight	830:835	arg1	times					844:848	three times a day	838:854	three times a day	838:854	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	3	102	theme	weight	830:835	arg1	%					814:814	10%	812:814	10% of total body weight, three times a day	812:854	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	10	103	theme	microbes	2170:2177	arg1	abundance					2146:2154	the abundance	2142:2154	the abundance of pathogenic microbes	2142:2177	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	1	104	theme	biochemical	367:377	arg1	compositions					379:390	biochemical compositions	367:390	biochemical compositions	367:390	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	3	105	theme	total	819:823	arg1	weight					830:835	total body weight	819:835	total body weight	819:835	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	3	105	theme	total	819:823	arg1	times					844:848	three times a day	838:854	three times a day	838:854	During the 90-day experiment trial, all shrimp larvae were fed their respective diets at 10% of total body weight, three times a day.
36836701	9	106	theme	genes	1691:1695	arg1	expression					1595:1604	the expression	1591:1604	the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei	1591:1901	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	0	107	theme	Administration	18:31	arg1	Impact					0:5	Impact	0:5	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).	0:191	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	9	108	theme	β	1698:1698	arg1	protein					1716:1722	β -Glucan-binding protein	1698:1722	β -Glucan-binding protein (β-Bgp)	1698:1730	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	108	theme	β	1698:1698	arg1	genes					1691:1695	immune-related genes	1676:1695	immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin)	1676:1786	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	9	108	theme	β	1698:1698	arg1	β-Bgp					1725:1729	β-Bgp	1725:1729	β-Bgp	1725:1729	At the end of the feeding experiment, the dietary supplementation of polysaccharide levels enhanced the expression of growth-related genes (Insulin-like growth factors (IGF-I, IGF-II), immune-related genes (β -Glucan-binding protein (β-Bgp), Prophenoloxidase (ProPO), Lysozyme (Lys), and Crustin), and stress genes (Superoxide dismutase (SOD) and Glutathione peroxidase (GPx) in the muscle tissue of L. vannamei.
36836701	8	109	theme	polysaccharide-treated	1437:1458	arg1	diets					1460:1464	polysaccharide-treated diets	1437:1464	polysaccharide-treated diets	1437:1464	of L. vannamei showed significant differences among polysaccharide-treated diets compared to the control.
36836701	0	110	theme	Seaweed	36:42	arg1	Polysaccharide					44:57	Seaweed Polysaccharide	36:57	Seaweed Polysaccharide	36:57	Impact of Dietary Administration of Seaweed Polysaccharide on Growth, Microbial Abundance, and Growth and Immune-Related Genes Expression of The Pacific Whiteleg Shrimp (Litopenaeus vannamei).
36836701	4	111	theme	different	901:909	arg1	levels					940:945	different seaweed polysaccharide (SWP) levels	901:945	different seaweed polysaccharide (SWP) levels	901:945	Three experimental diets were prepared with different seaweed polysaccharide (SWP) levels.
36836701	10	112	theme	kg-1	2129:2132	arg1	level					2116:2120	the incorporation level	2098:2120	the incorporation level of 3 g kg-1	2098:2132	However, the current study concluded that the inclusion rate of 2 g kg-1 of polysaccharide as a dietary additive administration enhanced both weight gain and survival rate of L. vannamei, while the incorporation level of 3 g kg-1 reduces the abundance of pathogenic microbes and enhances the growth-, immunity- and stress-related gene expressions of L. vannamei.
36836701	4	113	theme	polysaccharide	919:932	arg1	levels					940:945	different seaweed polysaccharide (SWP) levels	901:945	different seaweed polysaccharide (SWP) levels	901:945	Three experimental diets were prepared with different seaweed polysaccharide (SWP) levels.
36836701	2	114	with	shrimp	670:675	arg1	weight					693:698	an initial weight	682:698	an initial weight of (0.0017 ± 0.001 g)	682:720	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	2	114	with	shrimp	670:675	arg1	g					719:719	0.0017 ± 0.001 g	704:719	0.0017 ± 0.001 g	704:719	A total of 360 post-larvae of L. vannamei were randomly distributed into a 12-glass aquarium (40 L of each) at a stocking density of 30 shrimp with an initial weight of (0.0017 ± 0.001 g).
36836701	1	115	theme	Whiteleg	496:503	arg1	shrimp					505:510	the Pacific Whiteleg shrimp	484:510	the Pacific Whiteleg shrimp	484:510	This work aims to determine the impact of dietary supplementation of polysaccharide, extracted from brown seaweeds Sargassum dentifolium on growth indices, feed utilization, biochemical compositions, microbial abundance, expressions of growth and immunity-related genes, and stress genes of the Pacific Whiteleg shrimp Litopenaeus vannamei.
36836701	5	116	theme	polysaccharide	978:991	arg1	SWP0					1000:1003	SWP0	1000:1003	SWP0	1000:1003	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	116	theme	polysaccharide	978:991	arg1	level					993:997	no polysaccharide level	975:997	no polysaccharide level (SWP0)	975:1004	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36836701	5	117	theme	kg-1	1095:1098	arg1	diet					1100:1103	1, 2, and 3 g kg-1 diet	1081:1103	1, 2, and 3 g kg-1 diet	1081:1103	The basal control diet had no polysaccharide level (SWP0), while SWP1, SWP2, and SWP3 contained polysaccharides at concentrations of 1, 2, and 3 g kg-1 diet, respectively.
36523841	3	0	theme	gut	696:698	arg1	species					712:718	multiple gut Bacteroides species	687:718	multiple gut Bacteroides species	687:718	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	6	1	theme	mucosal	1287:1293	arg1	clade					1314:1318	the mucosal glycan utilization clade	1283:1318	the mucosal glycan utilization clade	1283:1318	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	1	2	theme	present	179:185	arg1	Glycosaminoglycans					153:170	Introduction Glycosaminoglycans	140:170	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer	140:206	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	2	theme	present	179:185	arg1	GAGs					173:176	GAGs	173:176	GAGs	173:176	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	2	theme	present	179:185	arg1	nutrients					223:231	nutrients	223:231	nutrients	223:231	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	5	3	from	BT_3328	957:963	arg1	combination					968:978	combination	968:978	combination with surface binding proteins BT_3329 and BT_3330	968:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	0	4	theme	locus	93:97	arg1	architecture					99:110	polysaccharide utilization locus architecture	66:110	polysaccharide utilization locus architecture of gut Bacteroides species	66:137	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	1	5	from	present	179:185	arg1	layer					202:206	the mucosal layer	190:206	the mucosal layer	190:206	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	2	6	theme	inflammatory	358:369	arg1	diseases					377:384	inflammatory bowel diseases	358:384	inflammatory bowel diseases	358:384	GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
36523841	0	7	theme	gut	115:117	arg1	species					131:137	gut Bacteroides species	115:137	gut Bacteroides species	115:137	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	2	8	theme	GAG	306:308	arg1	abundances					310:319	GAG abundances	306:319	GAG abundances	306:319	GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
36523841	2	9	theme	microbiota	522:531	arg1	members					503:509	members	503:509	members of the gut microbiota	503:531	GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
36523841	3	10	theme	growth	550:555	arg1	assays					557:562	growth assays	550:562	growth assays	550:562	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	5	11	with	combination	968:978	arg1	proteins					1001:1008	surface binding proteins BT_3329 and BT_3330	985:1028	surface binding proteins BT_3329 and BT_3330	985:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	11	with	combination	968:978	arg1	BT_3330					1022:1028	BT_3330	1022:1028	BT_3330	1022:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	11	with	combination	968:978	arg1	BT_3329					1010:1016	BT_3329	1010:1016	BT_3329	1010:1016	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	2	12	theme	bowel	371:375	arg1	diseases					377:384	inflammatory bowel diseases	358:384	inflammatory bowel diseases	358:384	GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
36523841	2	13	theme	gut	518:520	arg1	microbiota					522:531	the gut microbiota	514:531	the gut microbiota	514:531	GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
36523841	6	14	theme	compounds	1117:1125	arg1	degradation					1097:1107	degradation	1097:1107	degradation of both compounds	1097:1125	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	4	15	theme	Bacteroides	766:776	arg1	species					778:784	not all Bacteroides species	758:784	not all Bacteroides species able to degrade CS	758:803	Results and discussion We found that not all Bacteroides species able to degrade CS could also degrade HA, despite having lyases which act on both compounds.
36523841	2	16	theme	microbial	404:412	arg1	composition					414:424	microbial composition	404:424	microbial composition	404:424	GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
36523841	0	17	theme	Bacteroides	119:129	arg1	species					131:137	gut Bacteroides species	115:137	gut Bacteroides species	115:137	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	3	18	theme	Bacteroides	700:710	arg1	species					712:718	multiple gut Bacteroides species	687:718	multiple gut Bacteroides species	687:718	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	6	19	theme	other	1153:1157	arg1	Bacteroides					1159:1169	other Bacteroides	1153:1169	other Bacteroides	1153:1169	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	6	19	theme	other	1153:1157	arg1	non-degraders					1182:1194	non-degraders	1182:1194	non-degraders	1182:1194	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	1	20	theme	mucosal	194:200	arg1	layer					202:206	the mucosal layer	190:206	the mucosal layer	190:206	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	0	21	theme	niche	9:13	arg1	specificity					15:25	Nutrient niche specificity	0:25	Nutrient niche specificity for glycosaminoglycans	0:48	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	1	22	attach	present	179:185	arg1	layer					202:206	the mucosal layer	190:206	the mucosal layer	190:206	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	22	attach	present	179:185	arg2	nutrients					223:231	nutrients	223:231	nutrients	223:231	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	22	attach	present	179:185	arg2	GAGs					173:176	GAGs	173:176	GAGs	173:176	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	22	attach	present	179:185	arg2	Glycosaminoglycans					153:170	Introduction Glycosaminoglycans	140:170	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer	140:206	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	5	23	theme	model	902:906	arg1	thetaiotaomicron					929:944	the model organism Bacteroides thetaiotaomicron	898:944	the model organism Bacteroides thetaiotaomicron	898:944	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	0	24	theme	Nutrient	0:7	arg1	specificity					15:25	Nutrient niche specificity	0:25	Nutrient niche specificity for glycosaminoglycans	0:48	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	5	25	theme	organism	908:915	arg1	thetaiotaomicron					929:944	the model organism Bacteroides thetaiotaomicron	898:944	the model organism Bacteroides thetaiotaomicron	898:944	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	3	26	used	used	545:548	arg2	Methods					534:540	Methods	534:540	Methods We	534:543	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	1	27	theme	Bacteroides	293:303	arg1	bacteria					255:262	certain intestinal bacteria	236:262	certain intestinal bacteria	236:262	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	27	theme	Bacteroides	293:303	arg1	members					278:284	members	278:284	members of the Bacteroides	278:303	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	6	28	theme	cooperative	1213:1223	arg1	degradation					1225:1235	cooperative degradation	1213:1235	cooperative degradation as well as cross-feeding	1213:1260	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	5	29	theme	Bacteroides	917:927	arg1	thetaiotaomicron					929:944	the model organism Bacteroides thetaiotaomicron	898:944	the model organism Bacteroides thetaiotaomicron	898:944	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	30	theme	binding	993:999	arg1	proteins					1001:1008	surface binding proteins BT_3329 and BT_3330	985:1028	surface binding proteins BT_3329 and BT_3330	985:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	30	theme	binding	993:999	arg1	BT_3330					1022:1028	BT_3330	1022:1028	BT_3330	1022:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	30	theme	binding	993:999	arg1	BT_3329					1010:1016	BT_3329	1010:1016	BT_3329	1010:1016	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	31	theme	lyase	951:955	arg1	BT_3328					957:963	the lyase BT_3328	947:963	the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330	947:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	6	32	from	widespread	1269:1278	arg1	clade					1314:1318	the mucosal glycan utilization clade	1283:1318	the mucosal glycan utilization clade	1283:1318	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	2	33	theme	GAG	484:486	arg1	utilization					488:498	GAG utilization	484:498	GAG utilization	484:498	GAG abundances are altered in some diseases such as inflammatory bowel diseases, which may affect microbial composition and activity, and it is therefore important to understand GAG utilization by members of the gut microbiota.
36523841	0	34	theme	species	131:137	arg1	architecture					99:110	polysaccharide utilization locus architecture	66:110	polysaccharide utilization locus architecture of gut Bacteroides species	66:137	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	5	35	dep	proteins	1001:1008	arg1	proteins					1001:1008	surface binding proteins BT_3329 and BT_3330	985:1028	surface binding proteins BT_3329 and BT_3330	985:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	35	dep	proteins	1001:1008	arg1	BT_3330					1022:1028	BT_3330	1022:1028	BT_3330	1022:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	35	dep	proteins	1001:1008	arg1	BT_3329					1010:1016	BT_3329	1010:1016	BT_3329	1010:1016	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	3	36	dep	sulfate	631:637	arg1	ability					676:682	degradation ability	664:682	degradation ability	664:682	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	6	37	from	clade	1314:1318	arg1	widespread					1269:1278	widespread	1269:1278	widespread	1269:1278	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	5	38	from	BT_4411	1046:1052	arg1	combination					968:978	combination	968:978	combination with surface binding proteins BT_3329 and BT_3330	968:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	39	theme	HA	1070:1071	arg1	breakdown					1073:1081	HA breakdown	1070:1081	HA breakdown	1070:1081	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	1	40	used	used	215:218	arg2	nutrients					223:231	nutrients	223:231	nutrients	223:231	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	40	used	used	215:218	arg2	GAGs					173:176	GAGs	173:176	GAGs	173:176	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	40	used	used	215:218	arg2	Glycosaminoglycans					153:170	Introduction Glycosaminoglycans	140:170	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer	140:206	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	3	41	dep	Methods	534:540	arg1	We					542:543	We	542:543	We	542:543	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	3	42	theme	comparative	586:596	arg1	genomics					598:605	comparative genomics	586:605	comparative genomics	586:605	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	1	43	theme	Introduction	140:151	arg1	Glycosaminoglycans					153:170	Introduction Glycosaminoglycans	140:170	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer	140:206	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	43	theme	Introduction	140:151	arg1	GAGs					173:176	GAGs	173:176	GAGs	173:176	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	43	theme	Introduction	140:151	arg1	nutrients					223:231	nutrients	223:231	nutrients	223:231	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	5	44	theme	surface	985:991	arg1	proteins					1001:1008	surface binding proteins BT_3329 and BT_3330	985:1028	surface binding proteins BT_3329 and BT_3330	985:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	44	theme	surface	985:991	arg1	BT_3330					1022:1028	BT_3330	1022:1028	BT_3330	1022:1028	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	5	44	theme	surface	985:991	arg1	BT_3329					1010:1016	BT_3329	1010:1016	BT_3329	1010:1016	We propose that in the model organism Bacteroides thetaiotaomicron, the lyase BT_3328 in combination with surface binding proteins BT_3329 and BT_3330 and potentially BT_4411 are involved in HA breakdown.
36523841	6	45	theme	utilization	1302:1312	arg1	clade					1314:1318	the mucosal glycan utilization clade	1283:1318	the mucosal glycan utilization clade	1283:1318	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	3	46	theme	degradation	664:674	arg1	ability					676:682	degradation ability	664:682	degradation ability	664:682	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	4	47	theme	able	786:789	arg1	species					778:784	not all Bacteroides species	758:784	not all Bacteroides species able to degrade CS	758:803	Results and discussion We found that not all Bacteroides species able to degrade CS could also degrade HA, despite having lyases which act on both compounds.
36523841	4	48	dep	discussion	733:742	arg1	We					744:745	We	744:745	We	744:745	Results and discussion We found that not all Bacteroides species able to degrade CS could also degrade HA, despite having lyases which act on both compounds.
36523841	6	49	theme	public	1136:1141	arg1	goods					1143:1147	public goods	1136:1147	public goods for other Bacteroides, including non-degraders	1136:1194	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	6	50	theme	glycan	1295:1300	arg1	clade					1314:1318	the mucosal glycan utilization clade	1283:1318	the mucosal glycan utilization clade	1283:1318	Furthermore, degradation of both compounds provides public goods for other Bacteroides, including non-degraders, suggesting that cooperative degradation as well as cross-feeding may be widespread in the mucosal glycan utilization clade.
36523841	1	51	from	layer	202:206	arg1	present					179:185	present	179:185	present	179:185	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	52	theme	certain	236:242	arg1	bacteria					255:262	certain intestinal bacteria	236:262	certain intestinal bacteria	236:262	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	52	theme	certain	236:242	arg1	members					278:284	members	278:284	members of the Bacteroides	278:303	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	0	53	theme	utilization	81:91	arg1	architecture					99:110	polysaccharide utilization locus architecture	66:110	polysaccharide utilization locus architecture of gut Bacteroides species	66:137	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	1	54	theme	intestinal	244:253	arg1	bacteria					255:262	certain intestinal bacteria	236:262	certain intestinal bacteria	236:262	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	1	54	theme	intestinal	244:253	arg1	members					278:284	members	278:284	members of the Bacteroides	278:303	Introduction Glycosaminoglycans (GAGs) present in the mucosal layer can be used as nutrients by certain intestinal bacteria, particularly members of the Bacteroides.
36523841	0	55	theme	polysaccharide	66:79	arg1	architecture					99:110	polysaccharide utilization locus architecture	66:110	polysaccharide utilization locus architecture of gut Bacteroides species	66:137	Nutrient niche specificity for glycosaminoglycans is reflected in polysaccharide utilization locus architecture of gut Bacteroides species.
36523841	3	56	theme	multiple	687:694	arg1	species					712:718	multiple gut Bacteroides species	687:718	multiple gut Bacteroides species	687:718	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	3	57	theme	chondroitin	619:629	arg1	CS					640:641	CS	640:641	CS	640:641	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
36523841	3	57	theme	chondroitin	619:629	arg1	sulfate					631:637	chondroitin sulfate	619:637	chondroitin sulfate (CS)	619:642	Methods We used growth assays, transcriptomics, and comparative genomics to evaluate chondroitin sulfate (CS) and hyaluronan (HA) degradation ability by multiple gut Bacteroides species.
35495602	4	0	theme	cells	806:810	arg1	pinocytosis					782:792	pinocytosis	782:792	pinocytosis	782:792	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	0	theme	cells	806:810	arg1	proliferation					764:776	the proliferation	760:776	the proliferation	760:776	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	6	1	theme	protein	1324:1330	arg1	kinase					1332:1337	the mitogen-activated protein kinase	1302:1337	the mitogen-activated protein kinase	1302:1337	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	7	2	theme	fraction	1410:1417	arg1	higher					1432:1437	higher	1432:1437	higher	1432:1437	Notably, the immune activity of the F2 fraction was markedly higher than that of the crude polysaccharides.
35495602	7	2	theme	fraction	1410:1417	arg1	activity					1391:1398	the immune activity	1380:1398	the immune activity of the F2 fraction	1380:1417	Notably, the immune activity of the F2 fraction was markedly higher than that of the crude polysaccharides.
35495602	0	3	from	activity	18:25	arg1	macrophages					61:71	macrophages	61:71	macrophages	61:71	Immunostimulatory activity of soybean hull polysaccharide on macrophages.
35495602	4	4	theme	RAW264.7	797:804	arg1	cells					806:810	RAW264.7 cells	797:810	RAW264.7 cells	797:810	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	6	5	theme	mitogen-activated	1306:1322	arg1	kinase					1332:1337	the mitogen-activated protein kinase	1302:1337	the mitogen-activated protein kinase	1302:1337	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	6	6	theme	molecular	1150:1158	arg1	pattern					1160:1166	pathogen-associated molecular pattern	1130:1166	pathogen-associated molecular pattern	1130:1166	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	6	7	theme	western	1062:1068	arg1	blotting					1070:1077	western blotting	1062:1077	western blotting	1062:1077	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	6	8	theme	other	1083:1087	arg1	experiments					1089:1099	other experiments	1083:1099	other experiments	1083:1099	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	8	9	theme	SHP	1519:1521	arg1	fraction					1507:1514	the purified F2 fraction	1491:1514	the purified F2 fraction of SHP	1491:1521	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	8	9	theme	SHP	1519:1521	arg1	supplement					1555:1564	an effective nutritional supplement	1530:1564	an effective nutritional supplement for human disorders associated with low immunity	1530:1613	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	1	10	theme	Water-soluble	74:86	arg1	polysaccharide					88:101	Water-soluble polysaccharide	74:101	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography	74:179	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity.
35495602	8	11	theme	effective	1533:1541	arg1	supplement					1555:1564	an effective nutritional supplement	1530:1564	an effective nutritional supplement for human disorders associated with low immunity	1530:1613	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	8	11	theme	effective	1533:1541	arg1	fraction					1507:1514	the purified F2 fraction	1491:1514	the purified F2 fraction of SHP	1491:1521	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	7	12	theme	F2	1407:1408	arg1	fraction					1410:1417	the F2 fraction	1403:1417	the F2 fraction	1403:1417	Notably, the immune activity of the F2 fraction was markedly higher than that of the crude polysaccharides.
35495602	4	13	theme	tumor	865:869	arg1	factor					880:885	tumor necrosis factor (TNF)-α	865:893	tumor necrosis factor (TNF)-α	865:893	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	13	theme	tumor	865:869	arg1	oxide					853:857	nitric oxide	846:857	nitric oxide (NO)	846:862	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	13	theme	tumor	865:869	arg1	TNF					888:890	TNF	888:890	TNF	888:890	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	14	theme	RPMI	719:722	arg1	medium					724:729	RPMI medium	719:729	RPMI medium	719:729	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	6	15	theme	downstream	1288:1297	arg1	proteins					1279:1286	proteins	1279:1286	proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways	1279:1368	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	1	16	theme	molecular	218:226	arg1	characteristics					228:242	their molecular characteristics	212:242	their molecular characteristics	212:242	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity.
35495602	8	17	theme	nutritional	1543:1553	arg1	supplement					1555:1564	an effective nutritional supplement	1530:1564	an effective nutritional supplement for human disorders associated with low immunity	1530:1613	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	8	17	theme	nutritional	1543:1553	arg1	fraction					1507:1514	the purified F2 fraction	1491:1514	the purified F2 fraction of SHP	1491:1521	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	0	18	theme	Immunostimulatory	0:16	arg1	activity					18:25	Immunostimulatory activity	0:25	Immunostimulatory activity of soybean hull polysaccharide on macrophages	0:71	Immunostimulatory activity of soybean hull polysaccharide on macrophages.
35495602	5	19	theme	cells	1038:1042	arg1	regulation					1015:1024	the immune regulation	1004:1024	the immune regulation of RAW264.7 cells by SHP	1004:1049	Furthermore, flow cytometry demonstrated that CD11b and CD40 were involved in the immune regulation of RAW264.7 cells by SHP.
35495602	1	20	theme	soybean	117:123	arg1	hull					125:128	soybean hull	117:128	soybean hull	117:128	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity.
35495602	8	21	theme	human	1570:1574	arg1	disorders					1576:1584	human disorders	1570:1584	human disorders associated with low immunity	1570:1613	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	6	22	theme	pathogen-associated	1130:1148	arg1	pattern					1160:1166	pathogen-associated molecular pattern	1130:1166	pathogen-associated molecular pattern	1130:1166	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	0	23	theme	soybean	30:36	arg1	polysaccharide					43:56	soybean hull polysaccharide	30:56	soybean hull polysaccharide	30:56	Immunostimulatory activity of soybean hull polysaccharide on macrophages.
35495602	2	24	theme	hull	306:309	arg1	SHP					327:329	SHP	327:329	SHP	327:329	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	24	theme	hull	306:309	arg1	polysaccharide					311:324	soybean hull polysaccharide	298:324	soybean hull polysaccharide (SHP)	298:330	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	4	25	theme	nitric	846:851	arg1	factor					880:885	tumor necrosis factor (TNF)-α	865:893	tumor necrosis factor (TNF)-α	865:893	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	25	theme	nitric	846:851	arg1	NO					860:861	NO	860:861	NO	860:861	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	25	theme	nitric	846:851	arg1	oxide					853:857	nitric oxide	846:857	nitric oxide (NO)	846:862	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	25	theme	nitric	846:851	arg1	IL-6					920:923	IL-6	920:923	IL-6	920:923	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	25	theme	nitric	846:851	arg1	IL					909:910	interleukin (IL)-1β	896:914	interleukin (IL)-1β	896:914	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	2	26	theme	monosaccharide	430:443	arg1	compositions					445:456	their chemical and monosaccharide compositions	411:456	compositions	445:456	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	8	27	theme	purified	1495:1502	arg1	fraction					1507:1514	the purified F2 fraction	1491:1514	the purified F2 fraction of SHP	1491:1521	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	8	27	theme	purified	1495:1502	arg1	supplement					1555:1564	an effective nutritional supplement	1530:1564	an effective nutritional supplement for human disorders associated with low immunity	1530:1613	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	6	28	theme	expression	1258:1267	arg1	levels					1269:1274	the expression levels	1254:1274	the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways	1254:1368	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	2	29	dep	fractions	380:388	arg1	fractions					380:388	three main fractions	369:388	three main fractions (F1, F2 and F3)	369:404	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	29	dep	fractions	380:388	arg1	F3					402:403	F3	402:403	F3	402:403	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	29	dep	fractions	380:388	arg1	F2					395:396	F2	395:396	F2	395:396	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	29	dep	fractions	380:388	arg1	F1					391:392	F1	391:392	F1	391:392	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	0	30	theme	polysaccharide	43:56	arg1	activity					18:25	Immunostimulatory activity	0:25	Immunostimulatory activity of soybean hull polysaccharide on macrophages	0:71	Immunostimulatory activity of soybean hull polysaccharide on macrophages.
35495602	5	31	theme	flow	939:942	arg1	cytometry					944:952	flow cytometry	939:952	flow cytometry	939:952	Furthermore, flow cytometry demonstrated that CD11b and CD40 were involved in the immune regulation of RAW264.7 cells by SHP.
35495602	8	32	from	supplement	1555:1564	arg1	summary					1482:1488	summary	1482:1488	summary	1482:1488	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	6	33	theme	pathways	1361:1368	arg1	downstream					1288:1297	downstream	1288:1297	downstream	1288:1297	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	0	34	theme	hull	38:41	arg1	polysaccharide					43:56	soybean hull polysaccharide	30:56	soybean hull polysaccharide	30:56	Immunostimulatory activity of soybean hull polysaccharide on macrophages.
35495602	5	35	theme	immune	1008:1013	arg1	regulation					1015:1024	the immune regulation	1004:1024	the immune regulation of RAW264.7 cells by SHP	1004:1049	Furthermore, flow cytometry demonstrated that CD11b and CD40 were involved in the immune regulation of RAW264.7 cells by SHP.
35495602	4	36	theme	oxide	853:857	arg1	production					832:841	the production	828:841	the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6	828:923	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	3	37	theme	acid	590:593	arg1	levels					573:578	minor levels	567:578	minor levels of uronic acid (3.2%)	567:600	SHP was mainly composed of carbohydrates (64.3%), proteins (16.2%) and sulfates (12.5%), with minor levels of uronic acid (3.2%), and predominantly contained glucose and mannose as monosaccharides.
35495602	6	38	theme	proteins	1279:1286	arg1	levels					1269:1274	the expression levels	1254:1274	the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways	1254:1368	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	8	39	theme	low	1602:1604	arg1	immunity					1606:1613	low immunity	1602:1613	low immunity	1602:1613	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	4	40	theme	interleukin	896:906	arg1	IL					909:910	interleukin (IL)-1β	896:914	interleukin (IL)-1β	896:914	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	40	theme	interleukin	896:906	arg1	oxide					853:857	nitric oxide	846:857	nitric oxide (NO)	846:862	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	7	41	theme	crude	1456:1460	arg1	polysaccharides					1462:1476	the crude polysaccharides	1452:1476	the crude polysaccharides	1452:1476	Notably, the immune activity of the F2 fraction was markedly higher than that of the crude polysaccharides.
35495602	7	42	theme	immune	1384:1389	arg1	higher					1432:1437	higher	1432:1437	higher	1432:1437	Notably, the immune activity of the F2 fraction was markedly higher than that of the crude polysaccharides.
35495602	7	42	theme	immune	1384:1389	arg1	activity					1391:1398	the immune activity	1380:1398	the immune activity of the F2 fraction	1380:1417	Notably, the immune activity of the F2 fraction was markedly higher than that of the crude polysaccharides.
35495602	2	43	theme	present	283:289	arg1	study					291:295	the present study	279:295	the present study	279:295	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	1	44	dep	polysaccharide	88:101	arg1	fractionated					134:145	fractionated	134:145	fractionated using ion-exchange chromatography	134:179	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity.
35495602	1	44	dep	polysaccharide	88:101	arg1	isolated					103:110	isolated	103:110	isolated from soybean hull	103:128	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity.
35495602	6	45	theme	Toll-like	1204:1212	arg1	receptor					1214:1221	the Toll-like receptor 2	1200:1223	the Toll-like receptor 2	1200:1223	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	6	46	theme	κB	1358:1359	arg1	pathways					1361:1368	nuclear factor κB pathways	1343:1368	nuclear factor κB pathways	1343:1368	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	5	47	theme	RAW264.7	1029:1036	arg1	cells					1038:1042	RAW264.7 cells	1029:1042	RAW264.7 cells	1029:1042	Furthermore, flow cytometry demonstrated that CD11b and CD40 were involved in the immune regulation of RAW264.7 cells by SHP.
35495602	1	48	theme	ion-exchange	153:164	arg1	chromatography					166:179	ion-exchange chromatography	153:179	ion-exchange chromatography	153:179	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity.
35495602	6	49	theme	factor	1351:1356	arg1	pathways					1361:1368	nuclear factor κB pathways	1343:1368	nuclear factor κB pathways	1343:1368	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	6	50	theme	pattern	1160:1166	arg1	SHP					1115:1117	SHP	1115:1117	SHP	1115:1117	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	6	50	theme	pattern	1160:1166	arg1	type					1122:1125	a type	1120:1125	a type of pathogen-associated molecular pattern	1120:1166	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	3	51	theme	uronic	583:588	arg1	acid					590:593	uronic acid	583:593	uronic acid (3.2%)	583:600	SHP was mainly composed of carbohydrates (64.3%), proteins (16.2%) and sulfates (12.5%), with minor levels of uronic acid (3.2%), and predominantly contained glucose and mannose as monosaccharides.
35495602	3	51	theme	uronic	583:588	arg1	%					599:599	3.2%	596:599	3.2%	596:599	SHP was mainly composed of carbohydrates (64.3%), proteins (16.2%) and sulfates (12.5%), with minor levels of uronic acid (3.2%), and predominantly contained glucose and mannose as monosaccharides.
35495602	6	52	theme	nuclear	1343:1349	arg1	pathways					1361:1368	nuclear factor κB pathways	1343:1368	nuclear factor κB pathways	1343:1368	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
35495602	2	53	theme	main	375:378	arg1	fractions					380:388	three main fractions	369:388	three main fractions (F1, F2 and F3)	369:404	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	53	theme	main	375:378	arg1	F3					402:403	F3	402:403	F3	402:403	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	53	theme	main	375:378	arg1	F2					395:396	F2	395:396	F2	395:396	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	53	theme	main	375:378	arg1	F1					391:392	F1	391:392	F1	391:392	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	3	54	theme	minor	567:571	arg1	levels					573:578	minor levels	567:578	minor levels of uronic acid (3.2%)	567:600	SHP was mainly composed of carbohydrates (64.3%), proteins (16.2%) and sulfates (12.5%), with minor levels of uronic acid (3.2%), and predominantly contained glucose and mannose as monosaccharides.
35495602	1	55	theme	immunostimulating	248:264	arg1	activity					266:273	immunostimulating activity	248:273	immunostimulating activity	248:273	Water-soluble polysaccharide isolated from soybean hull and fractionated using ion-exchange chromatography were investigated to determine their molecular characteristics and immunostimulating activity.
35495602	8	56	theme	F2	1504:1505	arg1	fraction					1507:1514	the purified F2 fraction	1491:1514	the purified F2 fraction of SHP	1491:1521	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	8	56	theme	F2	1504:1505	arg1	supplement					1555:1564	an effective nutritional supplement	1530:1564	an effective nutritional supplement for human disorders associated with low immunity	1530:1613	In summary, the purified F2 fraction of SHP may be an effective nutritional supplement for human disorders associated with low immunity.
35495602	4	57	theme	necrosis	871:878	arg1	factor					880:885	tumor necrosis factor (TNF)-α	865:893	tumor necrosis factor (TNF)-α	865:893	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	57	theme	necrosis	871:878	arg1	oxide					853:857	nitric oxide	846:857	nitric oxide (NO)	846:862	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	4	57	theme	necrosis	871:878	arg1	TNF					888:890	TNF	888:890	TNF	888:890	Moreover, when compared with cells treated with RPMI medium, SHP was revealed to promote the proliferation and pinocytosis of RAW264.7 cells, and to enhance the production of nitric oxide (NO), tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6.
35495602	2	58	theme	soybean	298:304	arg1	SHP					327:329	SHP	327:329	SHP	327:329	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	2	58	theme	soybean	298:304	arg1	polysaccharide					311:324	soybean hull polysaccharide	298:324	soybean hull polysaccharide (SHP)	298:330	In the present study, soybean hull polysaccharide (SHP) was separated and purified to obtain three main fractions (F1, F2 and F3), and their chemical and monosaccharide compositions were analyzed.
35495602	6	59	theme	kinase	1332:1337	arg1	downstream					1288:1297	downstream	1288:1297	downstream	1288:1297	Moreover, western blotting and other experiments revealed that SHP, a type of pathogen-associated molecular pattern, was specifically recognized by the Toll-like receptor 2, which, in turn, upregulated the expression levels of proteins downstream of the mitogen-activated protein kinase and nuclear factor κB pathways.
37178244	6	0	theme	water	911:915	arg1	absorption					917:926	the water absorption	907:926	the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG	907:1028	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	9	1	dep	CONCLUSIONS	1410:1420	arg1	increased					1441:1449	increased	1441:1449	increased the water holding capacity of the BBG and gluten composite system	1441:1515	CONCLUSIONS In conclusion, BBG increased the water holding capacity of the BBG and gluten composite system.
37178244	6	2	dep	6.4	949:951	arg1	to					946:947	to	946:947	to	946:947	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	5	3	theme	system	761:766	arg1	structure					717:725	structure	717:725	structure	717:725	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	5	3	theme	system	761:766	arg1	properties					743:752	rheological properties	731:752	rheological properties	731:752	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	7	4	theme	binding	1092:1098	arg1	capacity					1100:1107	the binding capacity	1088:1107	the binding capacity of the system to weakly bound water	1088:1143	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	7	5	theme	thermal	1197:1203	arg1	temperature					1219:1229	the thermal decomposition temperature	1193:1229	the thermal decomposition temperature of the BBG and gluten composite system	1193:1268	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	5	6	theme	dynamic	636:642	arg1	analysis					656:663	dynamic rheological analysis	636:663	dynamic rheological analysis	636:663	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	9	7	theme	BBG	1485:1487	arg1	system					1510:1515	the BBG and gluten composite system	1481:1515	the BBG and gluten composite system	1481:1515	CONCLUSIONS In conclusion, BBG increased the water holding capacity of the BBG and gluten composite system.
37178244	9	8	theme	gluten	1493:1498	arg1	system					1510:1515	the BBG and gluten composite system	1481:1515	the BBG and gluten composite system	1481:1515	CONCLUSIONS In conclusion, BBG increased the water holding capacity of the BBG and gluten composite system.
37178244	4	9	theme	distribution	561:572	arg1	state					574:578	the distribution state	557:578	the distribution state of water	557:587	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	9	10	theme	system	1510:1515	arg1	capacity					1469:1476	the water holding capacity	1451:1476	the water holding capacity of the BBG and gluten composite system	1451:1515	CONCLUSIONS In conclusion, BBG increased the water holding capacity of the BBG and gluten composite system.
37178244	1	11	contain	possess	167:173	arg1	replacers					114:122	BACKGROUND Fat replacers	99:122	BACKGROUND Fat replacers prepared from polysaccharides and proteins	99:165	BACKGROUND Fat replacers prepared from polysaccharides and proteins possess functional properties of both polysaccharides and proteins.
37178244	1	11	contain	possess	167:173	arg2	properties					186:195	functional properties	175:195	functional properties of both polysaccharides and proteins	175:232	BACKGROUND Fat replacers prepared from polysaccharides and proteins possess functional properties of both polysaccharides and proteins.
37178244	5	12	theme	rheological	644:654	arg1	analysis					656:663	dynamic rheological analysis	636:663	dynamic rheological analysis	636:663	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	6	13	dep	RESULTS	769:775	arg1	results					781:787	The results	777:787	RESULTS The results	769:787	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	6	14	theme	weight	966:971	arg1	times					953:957	4.8 to 6.4 times	942:957	4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG	942:1028	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	10	15	theme	composite	1542:1550	arg1	system					1552:1557	the composite system	1538:1557	the composite system	1538:1557	With these changes, the composite system presented great potential for the preparation of polysaccharide-gluten fat replacer.
37178244	7	16	theme	BBG	1238:1240	arg1	temperature					1219:1229	the thermal decomposition temperature	1193:1229	the thermal decomposition temperature of the BBG and gluten composite system	1193:1268	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	10	17	theme	replacer	1634:1641	arg1	preparation					1593:1603	the preparation	1589:1603	the preparation of polysaccharide-gluten fat replacer	1589:1641	With these changes, the composite system presented great potential for the preparation of polysaccharide-gluten fat replacer.
37178244	2	18	theme	β-glucan	278:285	arg1	system					261:266	an aqueous system	250:266	an aqueous system of barley β-glucan (BBG) and gluten	250:302	In this study, an aqueous system of barley β-glucan (BBG) and gluten was prepared.
37178244	1	19	theme	functional	175:184	arg1	properties					186:195	functional properties	175:195	functional properties of both polysaccharides and proteins	175:232	BACKGROUND Fat replacers prepared from polysaccharides and proteins possess functional properties of both polysaccharides and proteins.
37178244	5	20	theme	rheological	731:741	arg1	properties					743:752	rheological properties	731:752	rheological properties	731:752	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	6	21	theme	extrusion	881:889	arg1	treatment					891:899	extrusion treatment	881:899	extrusion treatment	881:899	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	2	22	theme	barley	271:276	arg1	BBG					288:290	BBG	288:290	BBG	288:290	In this study, an aqueous system of barley β-glucan (BBG) and gluten was prepared.
37178244	2	22	theme	barley	271:276	arg1	β-glucan					278:285	barley β-glucan	271:285	barley β-glucan (BBG)	271:291	In this study, an aqueous system of barley β-glucan (BBG) and gluten was prepared.
37178244	3	23	theme	extrusion	372:380	arg1	modification					382:393	with/without extrusion modification	359:393	with/without extrusion modification	359:393	The interactions between BBG and gluten (with/without extrusion modification) were studied.
37178244	3	23	theme	extrusion	372:380	arg1	gluten					351:356	gluten	351:356	gluten (with/without extrusion modification)	351:394	The interactions between BBG and gluten (with/without extrusion modification) were studied.
37178244	6	24	dep	2.5	991:993	arg1	to					988:989	to	988:989	to	988:989	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	5	25	theme	fluorescence	601:612	arg1	analysis					626:633	fluorescence microscopic analysis	601:633	fluorescence microscopic analysis	601:633	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	9	26	theme	composite	1500:1508	arg1	system					1510:1515	the BBG and gluten composite system	1481:1515	the BBG and gluten composite system	1481:1515	CONCLUSIONS In conclusion, BBG increased the water holding capacity of the BBG and gluten composite system.
37178244	8	27	theme	BBG	1326:1328	arg1	solution					1330:1337	the BBG solution	1322:1337	the BBG solution	1322:1337	After the gluten was extruded and homogenized with the BBG solution, the appearance of the composite system was more uniform and delicate.
37178244	5	28	theme	microscopic	614:624	arg1	analysis					626:633	fluorescence microscopic analysis	601:633	fluorescence microscopic analysis	601:633	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	2	29	theme	gluten	297:302	arg1	system					261:266	an aqueous system	250:266	an aqueous system of barley β-glucan (BBG) and gluten	250:302	In this study, an aqueous system of barley β-glucan (BBG) and gluten was prepared.
37178244	0	30	theme	replacer	19:26	arg1	Preparation					0:10	Preparation	0:10	Preparation of fat replacer	0:26	Preparation of fat replacer utilizing gluten and barley β-glucan and the interaction between them.
37178244	4	31	theme	evaporation	525:535	arg1	process					537:543	the freezing-thawing and thermal evaporation process	492:543	the freezing-thawing and thermal evaporation process	492:543	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	10	32	theme	fat	1630:1632	arg1	replacer					1634:1641	polysaccharide-gluten fat replacer	1608:1641	polysaccharide-gluten fat replacer	1608:1641	With these changes, the composite system presented great potential for the preparation of polysaccharide-gluten fat replacer.
37178244	1	33	theme	polysaccharides	205:219	arg1	properties					186:195	functional properties	175:195	functional properties of both polysaccharides and proteins	175:232	BACKGROUND Fat replacers prepared from polysaccharides and proteins possess functional properties of both polysaccharides and proteins.
37178244	4	34	used	utilized	472:479	arg2	TGA					450:452	TGA	450:452	TGA	450:452	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	34	used	utilized	472:479	arg2	DSC					445:447	DSC	445:447	DSC	445:447	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	34	used	utilized	472:479	arg2	LF-NMR					459:464	LF-NMR	459:464	LF-NMR	459:464	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	34	used	utilized	472:479	arg2	methods					426:432	Triple analysis methods	410:432	Triple analysis methods	410:432	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	7	35	theme	triple	1035:1040	arg1	results					1051:1057	The triple analysis results	1031:1057	The triple analysis results	1031:1057	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	1	36	theme	BACKGROUND	99:108	arg1	replacers					114:122	BACKGROUND Fat replacers	99:122	BACKGROUND Fat replacers prepared from polysaccharides and proteins	99:165	BACKGROUND Fat replacers prepared from polysaccharides and proteins possess functional properties of both polysaccharides and proteins.
37178244	9	37	theme	holding	1461:1467	arg1	capacity					1469:1476	the water holding capacity	1451:1476	the water holding capacity of the BBG and gluten composite system	1451:1515	CONCLUSIONS In conclusion, BBG increased the water holding capacity of the BBG and gluten composite system.
37178244	7	38	theme	gluten	1246:1251	arg1	system					1263:1268	the BBG and gluten composite system	1234:1268	system	1263:1268	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	1	39	theme	proteins	225:232	arg1	properties					186:195	functional properties	175:195	functional properties of both polysaccharides and proteins	175:232	BACKGROUND Fat replacers prepared from polysaccharides and proteins possess functional properties of both polysaccharides and proteins.
37178244	0	40	theme	fat	15:17	arg1	replacer					19:26	fat replacer	15:26	fat replacer	15:26	Preparation of fat replacer utilizing gluten and barley β-glucan and the interaction between them.
37178244	4	41	theme	freezing-thawing	496:511	arg1	process					537:543	the freezing-thawing and thermal evaporation process	492:543	the freezing-thawing and thermal evaporation process	492:543	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	7	42	theme	system	1116:1121	arg1	capacity					1100:1107	the binding capacity	1088:1107	the binding capacity of the system to weakly bound water	1088:1143	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	6	43	theme	gluten	859:864	arg1	capacity					847:854	the water-holding capacity	829:854	the water-holding capacity of gluten	829:864	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	7	44	theme	composite	1253:1261	arg1	system					1263:1268	the BBG and gluten composite system	1234:1268	system	1263:1268	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	5	45	used	used	699:702	arg2	Meanwhile					590:598	Meanwhile	590:598	Meanwhile	590:598	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	5	45	used	used	699:702	arg2	analysis					685:692	electrophoresis analysis	669:692	electrophoresis analysis	669:692	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	5	45	used	used	699:702	arg2	analysis					626:633	fluorescence microscopic analysis	601:633	fluorescence microscopic analysis	601:633	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	5	45	used	used	699:702	arg2	analysis					656:663	dynamic rheological analysis	636:663	dynamic rheological analysis	636:663	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	7	46	theme	analysis	1042:1049	arg1	results					1051:1057	The triple analysis results	1031:1057	The triple analysis results	1031:1057	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	0	47	dep	gluten	38:43	arg1	β-glucan					56:63	β-glucan	56:63	β-glucan	56:63	Preparation of fat replacer utilizing gluten and barley β-glucan and the interaction between them.
37178244	1	48	theme	Fat	110:112	arg1	replacers					114:122	BACKGROUND Fat replacers	99:122	BACKGROUND Fat replacers prepared from polysaccharides and proteins	99:165	BACKGROUND Fat replacers prepared from polysaccharides and proteins possess functional properties of both polysaccharides and proteins.
37178244	4	49	theme	thermal	517:523	arg1	process					537:543	the freezing-thawing and thermal evaporation process	492:543	the freezing-thawing and thermal evaporation process	492:543	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	50	theme	analysis	417:424	arg1	methods					426:432	Triple analysis methods	410:432	Triple analysis methods	410:432	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	50	theme	analysis	417:424	arg1	DSC					445:447	DSC	445:447	DSC	445:447	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	50	theme	analysis	417:424	arg1	LF-NMR					459:464	LF-NMR	459:464	LF-NMR	459:464	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	50	theme	analysis	417:424	arg1	TGA					450:452	TGA	450:452	TGA	450:452	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	6	51	theme	water-holding	833:845	arg1	capacity					847:854	the water-holding capacity	829:854	the water-holding capacity of gluten	829:864	RESULTS The results showed that BBG significantly increased the water-holding capacity of gluten, regardless of extrusion treatment, with the water absorption reached about 4.8 to 6.4 times of its weight, which was one to 2.5 times higher than that without BBG.
37178244	5	52	theme	electrophoresis	669:683	arg1	analysis					685:692	electrophoresis analysis	669:692	electrophoresis analysis	669:692	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	4	53	theme	Triple	410:415	arg1	methods					426:432	Triple analysis methods	410:432	Triple analysis methods	410:432	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	53	theme	Triple	410:415	arg1	DSC					445:447	DSC	445:447	DSC	445:447	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	53	theme	Triple	410:415	arg1	LF-NMR					459:464	LF-NMR	459:464	LF-NMR	459:464	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	53	theme	Triple	410:415	arg1	TGA					450:452	TGA	450:452	TGA	450:452	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	8	54	theme	system	1372:1377	arg1	uniform					1388:1394	uniform	1388:1394	uniform	1388:1394	After the gluten was extruded and homogenized with the BBG solution, the appearance of the composite system was more uniform and delicate.
37178244	8	54	theme	system	1372:1377	arg1	appearance					1344:1353	the appearance	1340:1353	the appearance of the composite system	1340:1377	After the gluten was extruded and homogenized with the BBG solution, the appearance of the composite system was more uniform and delicate.
37178244	5	55	dep	structure	717:725	arg1	the					713:715	the	713:715	the	713:715	Meanwhile, fluorescence microscopic analysis, dynamic rheological analysis and electrophoresis analysis were used to study the structure and rheological properties of the system.
37178244	8	56	theme	composite	1362:1370	arg1	system					1372:1377	the composite system	1358:1377	the composite system	1358:1377	After the gluten was extruded and homogenized with the BBG solution, the appearance of the composite system was more uniform and delicate.
37178244	9	57	theme	water	1455:1459	arg1	capacity					1469:1476	the water holding capacity	1451:1476	the water holding capacity of the BBG and gluten composite system	1451:1515	CONCLUSIONS In conclusion, BBG increased the water holding capacity of the BBG and gluten composite system.
37178244	10	58	theme	polysaccharide-gluten	1608:1628	arg1	replacer					1634:1641	polysaccharide-gluten fat replacer	1608:1641	polysaccharide-gluten fat replacer	1608:1641	With these changes, the composite system presented great potential for the preparation of polysaccharide-gluten fat replacer.
37178244	2	59	theme	aqueous	253:259	arg1	system					261:266	an aqueous system	250:266	an aqueous system of barley β-glucan (BBG) and gluten	250:302	In this study, an aqueous system of barley β-glucan (BBG) and gluten was prepared.
37178244	7	60	theme	system	1263:1268	arg1	temperature					1219:1229	the thermal decomposition temperature	1193:1229	the thermal decomposition temperature of the BBG and gluten composite system	1193:1268	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	7	61	theme	decomposition	1205:1217	arg1	temperature					1219:1229	the thermal decomposition temperature	1193:1229	the thermal decomposition temperature of the BBG and gluten composite system	1193:1268	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	7	62	theme	gluten	1174:1179	arg1	aggregation					1159:1169	the aggregation	1155:1169	the aggregation of gluten	1155:1179	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	3	63	theme	with/without	359:370	arg1	modification					382:393	with/without extrusion modification	359:393	with/without extrusion modification	359:393	The interactions between BBG and gluten (with/without extrusion modification) were studied.
37178244	3	63	theme	with/without	359:370	arg1	gluten					351:356	gluten	351:356	gluten (with/without extrusion modification)	351:394	The interactions between BBG and gluten (with/without extrusion modification) were studied.
37178244	7	64	theme	bound	1133:1137	arg1	water					1139:1143	weakly bound water	1126:1143	weakly bound water	1126:1143	The triple analysis results suggested that BBG increased the binding capacity of the system to weakly bound water, hindered the aggregation of gluten and reduced the thermal decomposition temperature of the BBG and gluten composite system.
37178244	10	65	theme	great	1569:1573	arg1	potential					1575:1583	great potential	1569:1583	great potential for the preparation of polysaccharide-gluten fat replacer	1569:1641	With these changes, the composite system presented great potential for the preparation of polysaccharide-gluten fat replacer.
37178244	4	66	theme	water	583:587	arg1	process					537:543	the freezing-thawing and thermal evaporation process	492:543	the freezing-thawing and thermal evaporation process	492:543	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37178244	4	66	theme	water	583:587	arg1	state					574:578	the distribution state	557:578	the distribution state of water	557:587	Triple analysis methods, including DSC, TGA, and LF-NMR, were utilized to analyze the freezing-thawing and thermal evaporation process, as well as the distribution state of water.
37340801	9	0	theme	goji	1508:1511	arg1	berries					1513:1519	goji berries	1508:1519	goji berries from Zhongning, Qinghai, and Gansu in China	1508:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	3	1	theme	Qinghai	462:468	arg1	berries					470:476	Qinghai berries	462:476	Qinghai berries	462:476	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	1	2	theme	cell	216:219	arg1	berries					182:188	goji berries	177:188	goji berries (Lycium barbarum L.)	177:209	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	2	theme	cell	216:219	arg1	properties					226:235	the cell wall properties	212:235	the cell wall properties	212:235	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	7	3	theme	pectate	984:990	arg1	PEL					999:1001	PEL	999:1001	PEL	999:1001	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	7	3	theme	pectate	984:990	arg1	lyase					992:996	pectate lyase (PEL) and pectinesterase (PE) enzymes	984:1034	lyase	992:996	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	7	4	theme	analysis	952:959	arg1	results					961:967	The expression analysis results	937:967	The expression analysis results	937:967	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	9	5	from	Qinghai	1537:1543	arg1	features					1477:1484	structural features	1466:1484	structural features	1466:1484	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	5	from	Qinghai	1537:1543	arg1	analysis					1496:1503	gene analysis	1491:1503	gene analysis	1491:1503	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	5	from	Qinghai	1537:1543	arg1	berries					1513:1519	goji berries	1508:1519	goji berries from Zhongning, Qinghai, and Gansu in China	1508:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	5	from	Qinghai	1537:1543	arg1	composition					1453:1463	polysaccharide composition	1438:1463	polysaccharide composition	1438:1463	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	8	6	theme	wall	1310:1313	arg1	synthesis					1330:1338	cell wall polysaccharide synthesis	1305:1338	cell wall polysaccharide synthesis	1305:1338	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	9	7	from	Zhongning	1526:1534	arg1	features					1477:1484	structural features	1466:1484	structural features	1466:1484	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	7	from	Zhongning	1526:1534	arg1	analysis					1496:1503	gene analysis	1491:1503	gene analysis	1491:1503	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	7	from	Zhongning	1526:1534	arg1	berries					1513:1519	goji berries	1508:1519	goji berries from Zhongning, Qinghai, and Gansu in China	1508:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	7	from	Zhongning	1526:1534	arg1	composition					1453:1463	polysaccharide composition	1438:1463	polysaccharide composition	1438:1463	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	10	8	theme	genes	1638:1642	arg1	function					1616:1623	the molecular function	1602:1623	the molecular function of the major genes in the cell wall polysaccharides of goji berries	1602:1691	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	4	9	theme	principal	622:630	arg1	components					632:641	the principal components	618:641	the principal components of the cell wall polysaccharides in goji berries	618:690	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	9	theme	principal	622:630	arg1	Arabinose					569:577	Arabinose	569:577	Arabinose	569:577	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	9	theme	principal	622:630	arg1	galactose					580:588	galactose	580:588	galactose	580:588	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	9	theme	principal	622:630	arg1	acid					608:611	galacturonic acid	595:611	galacturonic acid	595:611	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	8	10	theme	galactose	1247:1255	arg1	metabolism					1257:1266	galactose metabolism	1247:1266	galactose metabolism	1247:1266	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	1	11	from	BACKGROUND	163:172	arg1	environment					250:260	ripening environment	241:260	ripening environment	241:260	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	11	from	BACKGROUND	163:172	arg1	berries					182:188	goji berries	177:188	goji berries (Lycium barbarum L.)	177:209	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	11	from	BACKGROUND	163:172	arg1	properties					226:235	the cell wall properties	212:235	the cell wall properties	212:235	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	11	from	BACKGROUND	163:172	arg1	Lycium					191:196	Lycium	191:196	Lycium	191:196	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	9	12	from	wall	1430:1433	arg1	features					1477:1484	structural features	1466:1484	structural features	1466:1484	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	12	from	wall	1430:1433	arg1	analysis					1496:1503	gene analysis	1491:1503	gene analysis	1491:1503	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	12	from	wall	1430:1433	arg1	composition					1453:1463	polysaccharide composition	1438:1463	polysaccharide composition	1438:1463	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	8	13	theme	metabolism	1173:1182	arg1	pathway					1184:1190	The starch and sucrose metabolism pathway	1150:1190	The starch and sucrose metabolism pathway	1150:1190	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	7	14	theme	acid	1096:1099	arg1	factors					1051:1057	major factors	1045:1057	major factors of higher galactose and galacturonic acid contents	1045:1108	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	10	15	theme	wall	1656:1659	arg1	polysaccharides					1661:1675	the cell wall polysaccharides	1647:1675	the cell wall polysaccharides of goji berries	1647:1691	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	0	16	dep	Lycium	121:126	arg1	L.					137:138	Lycium barbarum L.	121:138	Lycium barbarum L.	121:138	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	4	17	theme	galacturonic	595:606	arg1	components					632:641	the principal components	618:641	the principal components of the cell wall polysaccharides in goji berries	618:690	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	17	theme	galacturonic	595:606	arg1	Arabinose					569:577	Arabinose	569:577	Arabinose	569:577	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	17	theme	galacturonic	595:606	arg1	galactose					580:588	galactose	580:588	galactose	580:588	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	17	theme	galacturonic	595:606	arg1	acid					608:611	galacturonic acid	595:611	galacturonic acid	595:611	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	18	theme	polysaccharides	660:674	arg1	components					632:641	the principal components	618:641	the principal components of the cell wall polysaccharides in goji berries	618:690	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	18	theme	polysaccharides	660:674	arg1	Arabinose					569:577	Arabinose	569:577	Arabinose	569:577	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	18	theme	polysaccharides	660:674	arg1	galactose					580:588	galactose	580:588	galactose	580:588	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	4	18	theme	polysaccharides	660:674	arg1	acid					608:611	galacturonic acid	595:611	galacturonic acid	595:611	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	0	19	theme	wall	85:88	arg1	polysaccharides					90:104	cell wall polysaccharides	80:104	cell wall polysaccharides	80:104	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	5	20	from	content	709:715	arg1	Zhongning					733:741	Zhongning	733:741	Zhongning	733:741	Among them, the content of galactose in Zhongning was significantly highest (P < 0.05).
37340801	9	21	from	analysis	1496:1503	arg1	Qinghai					1537:1543	Qinghai	1537:1543	Qinghai	1537:1543	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	21	from	analysis	1496:1503	arg1	Gansu					1550:1554	Gansu	1550:1554	Gansu	1550:1554	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	21	from	analysis	1496:1503	arg1	Zhongning					1526:1534	Zhongning	1526:1534	Zhongning	1526:1534	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	21	from	analysis	1496:1503	arg1	berries					1513:1519	goji berries	1508:1519	goji berries from Zhongning, Qinghai, and Gansu in China	1508:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	6	22	theme	expressed	857:865	arg1	EG					882:883	EG	882:883	EG	882:883	Interestingly, we found that highly expressed β-glucosidase (BGL) and lowly expressed endoglucanase (EG) led to cellulose accumulation by RNA-seq analysis.
37340801	6	22	theme	expressed	857:865	arg1	endoglucanase					867:879	lowly expressed endoglucanase	851:879	lowly expressed endoglucanase (EG)	851:884	Interestingly, we found that highly expressed β-glucosidase (BGL) and lowly expressed endoglucanase (EG) led to cellulose accumulation by RNA-seq analysis.
37340801	4	23	theme	goji	679:682	arg1	berries					684:690	goji berries	679:690	goji berries	679:690	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	3	24	dep	higher	452:457	arg1	P					487:487	P < 0.01	487:494	P < 0.01	487:494	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	3	24	dep	higher	452:457	arg1	%					484:484	13.87%	479:484	13.87%	479:484	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	0	25	theme	Transcriptome	0:12	arg1	analysis					14:21	Transcriptome analysis	0:21	Transcriptome analysis	0:21	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	9	26	from	features	1477:1484	arg1	Qinghai					1537:1543	Qinghai	1537:1543	Qinghai	1537:1543	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	26	from	features	1477:1484	arg1	Gansu					1550:1554	Gansu	1550:1554	Gansu	1550:1554	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	26	from	features	1477:1484	arg1	Zhongning					1526:1534	Zhongning	1526:1534	Zhongning	1526:1534	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	26	from	features	1477:1484	arg1	berries					1513:1519	goji berries	1508:1519	goji berries from Zhongning, Qinghai, and Gansu in China	1508:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	7	27	dep	galactose	1069:1077	arg1	contents					1101:1108	contents	1101:1108	contents	1101:1108	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	4	28	theme	cell	650:653	arg1	polysaccharides					660:674	the cell wall polysaccharides	646:674	the cell wall polysaccharides	646:674	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	6	29	theme	RNA-seq	919:925	arg1	analysis					927:934	RNA-seq analysis	919:934	RNA-seq analysis	919:934	Interestingly, we found that highly expressed β-glucosidase (BGL) and lowly expressed endoglucanase (EG) led to cellulose accumulation by RNA-seq analysis.
37340801	0	30	theme	related	37:43	arg1	genes					31:35	genes	31:35	genes related to the synthesis and metabolism of cell wall polysaccharides	31:104	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	8	31	dep	pathway	1184:1190	arg1	pathway					1268:1274	pathway	1268:1274	pathway	1268:1274	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	10	32	theme	berries	1685:1691	arg1	polysaccharides					1661:1675	the cell wall polysaccharides	1647:1675	the cell wall polysaccharides of goji berries	1647:1691	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	7	33	theme	pectinesterase	1008:1021	arg1	enzymes					1028:1034	pectate lyase (PEL) and pectinesterase (PE) enzymes	984:1034	enzymes	1028:1034	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	7	33	theme	pectinesterase	1008:1021	arg1	PE					1024:1025	PE	1024:1025	PE	1024:1025	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	9	34	dep	CONCLUSION	1356:1365	arg1	study					1375:1379	this study	1370:1379	this study	1370:1379	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	35	theme	structural	1466:1475	arg1	features					1477:1484	structural features	1466:1484	structural features	1466:1484	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	3	36	theme	sugar	434:438	arg1	content					440:446	total sugar content	428:446	total sugar content	428:446	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	9	37	theme	gene	1491:1494	arg1	analysis					1496:1503	gene analysis	1491:1503	gene analysis	1491:1503	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	10	38	theme	solid	1707:1711	arg1	foundation					1713:1722	a solid foundation	1705:1722	a solid foundation for further study	1705:1740	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	8	39	theme	cell	1305:1308	arg1	synthesis					1330:1338	cell wall polysaccharide synthesis	1305:1338	cell wall polysaccharide synthesis	1305:1338	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	7	40	theme	galactose	1069:1077	arg1	factors					1051:1057	major factors	1045:1057	major factors of higher galactose and galacturonic acid contents	1045:1108	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	10	41	theme	molecular	1606:1614	arg1	function					1616:1623	the molecular function	1602:1623	the molecular function of the major genes in the cell wall polysaccharides of goji berries	1602:1691	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	1	42	theme	wall	221:224	arg1	berries					182:188	goji berries	177:188	goji berries (Lycium barbarum L.)	177:209	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	42	theme	wall	221:224	arg1	properties					226:235	the cell wall properties	212:235	the cell wall properties	212:235	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	10	43	theme	major	1632:1636	arg1	genes					1638:1642	the major genes	1628:1642	the major genes	1628:1642	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	3	44	dep	peaked	524:529	arg1	P					558:558	P < 0.05	558:565	P < 0.05	558:565	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	3	44	dep	peaked	524:529	arg1	%					555:555	28%	553:555	28%	553:555	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	7	45	theme	expression	941:950	arg1	results					961:967	The expression analysis results	937:967	The expression analysis results	937:967	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	1	46	theme	goji	177:180	arg1	environment					250:260	ripening environment	241:260	ripening environment	241:260	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	46	theme	goji	177:180	arg1	berries					182:188	goji berries	177:188	goji berries (Lycium barbarum L.)	177:209	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	46	theme	goji	177:180	arg1	properties					226:235	the cell wall properties	212:235	the cell wall properties	212:235	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	46	theme	goji	177:180	arg1	Lycium					191:196	Lycium	191:196	Lycium	191:196	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	8	47	theme	polysaccharide	1315:1328	arg1	synthesis					1330:1338	cell wall polysaccharide synthesis	1305:1338	cell wall polysaccharide synthesis	1305:1338	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	10	48	theme	cell	1651:1654	arg1	polysaccharides					1661:1675	the cell wall polysaccharides	1647:1675	the cell wall polysaccharides of goji berries	1647:1691	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	3	49	theme	cellulose	506:514	arg1	content					516:522	cellulose content	506:522	cellulose content	506:522	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	6	50	theme	expressed	817:825	arg1	β-glucosidase					827:839	highly expressed β-glucosidase	810:839	highly expressed β-glucosidase (BGL)	810:845	Interestingly, we found that highly expressed β-glucosidase (BGL) and lowly expressed endoglucanase (EG) led to cellulose accumulation by RNA-seq analysis.
37340801	6	50	theme	expressed	817:825	arg1	BGL					842:844	BGL	842:844	BGL	842:844	Interestingly, we found that highly expressed β-glucosidase (BGL) and lowly expressed endoglucanase (EG) led to cellulose accumulation by RNA-seq analysis.
37340801	0	51	theme	various	146:152	arg1	regions					154:160	various regions	146:160	various regions	146:160	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	10	52	theme	goji	1680:1683	arg1	berries					1685:1691	goji berries	1680:1691	goji berries	1680:1691	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	9	53	from	composition	1453:1463	arg1	Qinghai					1537:1543	Qinghai	1537:1543	Qinghai	1537:1543	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	53	from	composition	1453:1463	arg1	Gansu					1550:1554	Gansu	1550:1554	Gansu	1550:1554	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	53	from	composition	1453:1463	arg1	Zhongning					1526:1534	Zhongning	1526:1534	Zhongning	1526:1534	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	53	from	composition	1453:1463	arg1	berries					1513:1519	goji berries	1508:1519	goji berries from Zhongning, Qinghai, and Gansu in China	1508:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	6	54	theme	cellulose	893:901	arg1	accumulation					903:914	cellulose accumulation	893:914	cellulose accumulation by RNA-seq analysis	893:934	Interestingly, we found that highly expressed β-glucosidase (BGL) and lowly expressed endoglucanase (EG) led to cellulose accumulation by RNA-seq analysis.
37340801	8	55	theme	starch	1154:1159	arg1	pathway					1184:1190	The starch and sucrose metabolism pathway	1150:1190	The starch and sucrose metabolism pathway	1150:1190	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	7	56	theme	galacturonic	1083:1094	arg1	acid					1096:1099	galacturonic acid	1083:1099	galacturonic acid	1083:1099	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	3	57	theme	Zhongning	534:542	arg1	berries					544:550	Zhongning berries	534:550	Zhongning berries	534:550	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	5	58	theme	galactose	720:728	arg1	highest					761:767	highest	761:767	highest	761:767	Among them, the content of galactose in Zhongning was significantly highest (P < 0.05).
37340801	5	58	theme	galactose	720:728	arg1	content					709:715	the content	705:715	the content of galactose in Zhongning	705:741	Among them, the content of galactose in Zhongning was significantly highest (P < 0.05).
37340801	1	59	dep	Lycium	191:196	arg1	L.					207:208	Lycium barbarum L.	191:208	Lycium barbarum L.	191:208	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	8	60	theme	sucrose	1165:1171	arg1	metabolism					1173:1182	sucrose metabolism	1165:1182	sucrose metabolism	1165:1182	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	7	61	theme	higher	1062:1067	arg1	galactose					1069:1077	higher galactose	1062:1077	higher galactose	1062:1077	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	0	62	theme	cell	80:83	arg1	polysaccharides					90:104	cell wall polysaccharides	80:104	cell wall polysaccharides	80:104	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	1	63	theme	economic	297:304	arg1	benefits					306:313	their economic benefits	291:313	their economic benefits	291:313	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	10	64	from	function	1616:1623	arg1	polysaccharides					1661:1675	the cell wall polysaccharides	1647:1675	the cell wall polysaccharides of goji berries	1647:1691	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	0	65	theme	polysaccharides	90:104	arg1	metabolism					66:75	metabolism	66:75	metabolism	66:75	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	0	65	theme	polysaccharides	90:104	arg1	synthesis					52:60	synthesis	52:60	synthesis	52:60	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	8	66	dep	starch	1154:1159	arg1	The					1150:1152	The	1150:1152	The	1150:1152	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	9	67	from	Gansu	1550:1554	arg1	features					1477:1484	structural features	1466:1484	structural features	1466:1484	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	67	from	Gansu	1550:1554	arg1	China					1559:1563	China	1559:1563	China	1559:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	67	from	Gansu	1550:1554	arg1	analysis					1496:1503	gene analysis	1491:1503	gene analysis	1491:1503	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	67	from	Gansu	1550:1554	arg1	berries					1513:1519	goji berries	1508:1519	goji berries from Zhongning, Qinghai, and Gansu in China	1508:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	9	67	from	Gansu	1550:1554	arg1	composition					1453:1463	polysaccharide composition	1438:1463	polysaccharide composition	1438:1463	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	4	68	theme	wall	655:658	arg1	polysaccharides					660:674	the cell wall polysaccharides	646:674	the cell wall polysaccharides	646:674	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	8	69	theme	pentose	1193:1199	arg1	pathway					1234:1240	pentose and glucuronate interconversions pathway	1193:1240	pathway	1234:1240	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	7	70	theme	major	1045:1049	arg1	factors					1051:1057	major factors	1045:1057	major factors of higher galactose and galacturonic acid contents	1045:1108	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	8	71	theme	glucuronate	1205:1215	arg1	pathway					1234:1240	pentose and glucuronate interconversions pathway	1193:1240	pathway	1234:1240	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	2	72	theme	cell	354:357	arg1	wall					359:362	the cell wall	350:362	the cell wall	350:362	However, the mechanism underlying the cell wall remains to be fully elucidated.
37340801	7	73	from	factors	1051:1057	arg1	Zhongning					1113:1121	Zhongning	1113:1121	Zhongning than in Qinghai and Gansu	1113:1147	The expression analysis results suggested that pectate lyase (PEL) and pectinesterase (PE) enzymes could be major factors of higher galactose and galacturonic acid contents in Zhongning than in Qinghai and Gansu.
37340801	1	74	theme	ripening	241:248	arg1	environment					250:260	ripening environment	241:260	ripening environment	241:260	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	1	74	theme	ripening	241:248	arg1	berries					182:188	goji berries	177:188	goji berries (Lycium barbarum L.)	177:209	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	4	75	from	components	632:641	arg1	berries					684:690	goji berries	679:690	goji berries	679:690	Arabinose, galactose, and galacturonic acid were the principal components of the cell wall polysaccharides in goji berries.
37340801	9	76	theme	polysaccharide	1438:1451	arg1	composition					1453:1463	polysaccharide composition	1438:1463	polysaccharide composition	1438:1463	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	8	77	theme	interconversions	1217:1232	arg1	pathway					1234:1240	pentose and glucuronate interconversions pathway	1193:1240	pathway	1234:1240	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
37340801	3	78	theme	total	428:432	arg1	content					440:446	total sugar content	428:446	total sugar content	428:446	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	0	79	dep	synthesis	52:60	arg1	the					48:50	the	48:50	the	48:50	Transcriptome analysis reveals genes related to the synthesis and metabolism of cell wall polysaccharides in goji berry (Lycium barbarum L.) from various regions.
37340801	1	80	theme	fruit	273:277	arg1	quality					279:285	the fruit quality	269:285	the fruit quality	269:285	BACKGROUND In goji berries (Lycium barbarum L.), the cell wall properties and ripening environment affect the fruit quality and their economic benefits.
37340801	3	81	dep	RESULTS	396:402	arg1	showed					416:421	showed	416:421	showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05)	416:566	RESULTS The results showed that total sugar content was higher in Qinghai berries (13.87%, P < 0.01), whereas cellulose content peaked in Zhongning berries (28%, P < 0.05).
37340801	10	82	theme	further	1728:1734	arg1	study					1736:1740	further study	1728:1740	further study	1728:1740	These results might help to clarify the molecular function of the major genes in the cell wall polysaccharides of goji berries and provide a solid foundation for further study.
37340801	5	83	dep	highest	761:767	arg1	P					770:770	P < 0.05	770:777	P < 0.05	770:777	Among them, the content of galactose in Zhongning was significantly highest (P < 0.05).
37340801	9	84	theme	cell	1425:1428	arg1	wall					1430:1433	the cell wall	1421:1433	the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China	1421:1563	CONCLUSION In this study, we aimed to provide some insights into the cell wall on polysaccharide composition, structural features, and gene analysis in goji berries from Zhongning, Qinghai, and Gansu in China.
37340801	8	85	theme	significant	1285:1295	arg1	role					1297:1300	a significant role	1283:1300	a significant role	1283:1300	The starch and sucrose metabolism pathway, pentose and glucuronate interconversions pathway, and galactose metabolism pathway played a significant role in cell wall polysaccharide synthesis and metabolism.
36015428	3	0	theme	spectrometry	501:512	arg1	technique					514:522	a liquid chromatography-mass spectrometry technique	472:522	a liquid chromatography-mass spectrometry technique	472:522	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	1	from	profiling	543:551	arg1	roots					589:593	roots	589:593	roots	589:593	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	1	from	profiling	543:551	arg1	herbs					579:583	herbs	579:583	herbs	579:583	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	6	2	dep	new	1318:1320	arg1	active					1335:1340	active	1335:1340	active	1335:1340	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	1	3	used	used	319:322	arg2	they					310:313	they	310:313	they	310:313	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	3	4	theme	technique	514:522	arg1	application					457:467	the application	453:467	the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides	453:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	5	theme	acid	696:699	arg1	derivatives					701:711	benzoic acid derivatives	688:711	benzoic acid derivatives	688:711	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	4	6	theme	additive	899:906	arg1	complexes					932:940	additive arabinogalactan-protein complexes	899:940	additive arabinogalactan-protein complexes	899:940	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	5	7	theme	extracts	1010:1017	arg1	potential					985:993	The antioxidant potential	969:993	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides	969:1091	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	4	8	theme	Water-soluble	756:768	arg1	pectins					886:892	mostly pectins	879:892	mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds	879:966	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	4	8	theme	Water-soluble	756:768	arg1	polysaccharides					770:784	Water-soluble polysaccharides	756:784	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides	756:842	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	6	9	theme	valuable	1298:1305	arg1	plants					1307:1312	valuable plants	1298:1312	valuable plants for new biologically active products	1298:1349	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	0	10	theme	austriacum	93:102	arg1	Polysaccharides					43:57	Polysaccharides	43:57	Polysaccharides	43:57	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	0	10	theme	austriacum	93:102	arg1	Bioactivity					64:74	Bioactivity	64:74	Bioactivity	64:74	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	0	10	theme	austriacum	93:102	arg1	Compounds					32:40	Phenolic Compounds	23:40	Phenolic Compounds	23:40	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	3	11	theme	benzoic	688:694	arg1	derivatives					701:711	benzoic acid derivatives	688:711	benzoic acid derivatives	688:711	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	12	theme	methanol	556:563	arg1	extracts					565:572	methanol extracts	556:572	methanol extracts from herbs and roots of D. austriacum and D. botryoides	556:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	0	13	theme	botryoides	122:131	arg1	Polysaccharides					43:57	Polysaccharides	43:57	Polysaccharides	43:57	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	0	13	theme	botryoides	122:131	arg1	Bioactivity					64:74	Bioactivity	64:74	Bioactivity	64:74	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	0	13	theme	botryoides	122:131	arg1	Compounds					32:40	Phenolic Compounds	23:40	Phenolic Compounds	23:40	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	7	14	dep	D.	1467:1468	arg1	austriacum					1470:1479	D. austriacum	1467:1479	D. austriacum	1467:1479	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	7	14	dep	D.	1467:1468	arg1	botryoides					1488:1497	botryoides	1488:1497	botryoides	1488:1497	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	7	15	theme	metabolites	1421:1431	arg1	study					1400:1404	the first study	1390:1404	the first study of whole plant metabolites	1390:1431	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	7	15	theme	metabolites	1421:1431	arg1	activity					1455:1462	their antioxidant activity	1437:1462	their antioxidant activity in D. austriacum and D. botryoides	1437:1497	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	7	15	theme	metabolites	1421:1431	arg1	this					1382:1385	this	1382:1385	this	1382:1385	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	3	16	theme	metabolite	532:541	arg1	profiling					543:551	the metabolite profiling	528:551	the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides	528:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	4	17	with	pectins	886:892	arg1	complexes					932:940	additive arabinogalactan-protein complexes	899:940	additive arabinogalactan-protein complexes	899:940	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	4	17	with	pectins	886:892	arg1	compounds					958:966	starch-like compounds	946:966	starch-like compounds	946:966	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	2	18	theme	chemical	385:392	arg1	composition					394:404	the chemical composition	381:404	the chemical composition of these Dracocephalum species	381:435	There are no data on the chemical composition of these Dracocephalum species.
36015428	6	19	theme	new	1318:1320	arg1	products					1342:1349	new biologically active products	1318:1349	new biologically active products	1318:1349	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	4	20	theme	herbs	793:797	arg1	pectins					886:892	mostly pectins	879:892	mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds	879:966	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	4	20	theme	herbs	793:797	arg1	polysaccharides					770:784	Water-soluble polysaccharides	756:784	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides	756:842	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	7	21	from	study	1400:1404	arg1	D.					1467:1468	D.	1467:1468	D.	1467:1468	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	7	21	from	study	1400:1404	arg1	D.					1485:1486	D.	1485:1486	D.	1485:1486	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	4	22	dep	D.	812:813	arg1	austriacum					815:824	D. austriacum	812:824	D. austriacum	812:824	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	4	22	dep	D.	812:813	arg1	botryoides					833:842	botryoides	833:842	botryoides	833:842	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	5	23	theme	phenolics	1049:1057	arg1	polysaccharides					1077:1091	water-soluble polysaccharides	1063:1091	water-soluble polysaccharides	1063:1091	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	5	23	theme	phenolics	1049:1057	arg1	extracts					1010:1017	the studied extracts	998:1017	the studied extracts of Dracocephalum and selected phenolics	998:1057	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	3	24	dep	D.	598:599	arg1	austriacum					601:610	D. austriacum	598:610	D. austriacum	598:610	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	24	dep	D.	598:599	arg1	botryoides					619:628	botryoides	619:628	botryoides	619:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	0	25	theme	Caucasian	0:8	arg1	Dragonheads					10:20	Caucasian Dragonheads	0:20	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.	0:132	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	5	26	theme	polysaccharides	1077:1091	arg1	potential					985:993	The antioxidant potential	969:993	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides	969:1091	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	6	27	theme	D.	1221:1222	arg1	roots					1212:1216	roots	1212:1216	roots	1212:1216	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	27	theme	D.	1221:1222	arg1	herbs					1202:1206	herbs	1202:1206	herbs	1202:1206	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	27	theme	D.	1221:1222	arg1	sources					1262:1268	rich sources	1257:1268	rich sources of metabolites	1257:1283	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	3	28	from	roots	589:593	arg1	profiling					543:551	the metabolite profiling	528:551	the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides	528:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	28	from	roots	589:593	arg1	extracts					565:572	methanol extracts	556:572	methanol extracts from herbs and roots of D. austriacum and D. botryoides	556:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	6	29	dep	D.	1221:1222	arg1	austriacum					1224:1233	D. austriacum	1221:1233	D. austriacum	1221:1233	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	29	dep	D.	1221:1222	arg1	botryoides					1242:1251	botryoides	1242:1251	botryoides	1242:1251	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	0	30	theme	Phenolic	23:30	arg1	Compounds					32:40	Phenolic Compounds	23:40	Phenolic Compounds	23:40	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	7	31	theme	first	1394:1398	arg1	study					1400:1404	the first study	1390:1404	the first study of whole plant metabolites	1390:1431	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	7	31	theme	first	1394:1398	arg1	this					1382:1385	this	1382:1385	this	1382:1385	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	3	32	theme	extracts	565:572	arg1	profiling					543:551	the metabolite profiling	528:551	the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides	528:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	33	theme	D.	598:599	arg1	roots					589:593	roots	589:593	roots	589:593	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	33	theme	D.	598:599	arg1	herbs					579:583	herbs	579:583	herbs	579:583	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	2	34	theme	Dracocephalum	415:427	arg1	species					429:435	these Dracocephalum species	409:435	these Dracocephalum species	409:435	There are no data on the chemical composition of these Dracocephalum species.
36015428	2	35	theme	species	429:435	arg1	composition					394:404	the chemical composition	381:404	the chemical composition of these Dracocephalum species	381:435	There are no data on the chemical composition of these Dracocephalum species.
36015428	5	36	theme	Dracocephalum	1022:1034	arg1	polysaccharides					1077:1091	water-soluble polysaccharides	1063:1091	water-soluble polysaccharides	1063:1091	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	5	36	theme	Dracocephalum	1022:1034	arg1	extracts					1010:1017	the studied extracts	998:1017	the studied extracts of Dracocephalum and selected phenolics	998:1057	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	1	37	dep	Dracocephalum	170:182	arg1	L.					195:196	Dracocephalum austriacum L.	170:196	Dracocephalum austriacum L.	170:196	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	1	38	theme	Dracocephalum	228:240	arg1	family					259:264	the Dracocephalum genus (Lamiaceae family)	224:265	the Dracocephalum genus (Lamiaceae family)	224:265	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	5	39	theme	water-soluble	1063:1075	arg1	polysaccharides					1077:1091	water-soluble polysaccharides	1063:1091	water-soluble polysaccharides	1063:1091	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	1	40	theme	local	345:349	arg1	cuisine					351:357	local cuisine	345:357	local cuisine	345:357	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	6	41	theme	rich	1257:1260	arg1	roots					1212:1216	roots	1212:1216	roots	1212:1216	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	41	theme	rich	1257:1260	arg1	herbs					1202:1206	herbs	1202:1206	herbs	1202:1206	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	41	theme	rich	1257:1260	arg1	sources					1262:1268	rich sources	1257:1268	rich sources of metabolites	1257:1283	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	1	42	theme	genus	242:246	arg1	family					259:264	the Dracocephalum genus (Lamiaceae family)	224:265	the Dracocephalum genus (Lamiaceae family)	224:265	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	1	43	from	distribution	274:285	arg1	Caucasus					294:301	the Caucasus	290:301	the Caucasus	290:301	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	5	44	theme	ion	1151:1153	arg1	assays					1165:1170	radical-scavenging and ferrous (II) ion chelating assays	1115:1170	radical-scavenging and ferrous (II) ion chelating assays	1115:1170	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	4	45	theme	D.	830:831	arg1	roots					803:807	roots	803:807	roots	803:807	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	4	45	theme	D.	830:831	arg1	herbs					793:797	herbs	793:797	herbs	793:797	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	5	46	theme	chelating	1155:1163	arg1	assays					1165:1170	radical-scavenging and ferrous (II) ion chelating assays	1115:1170	radical-scavenging and ferrous (II) ion chelating assays	1115:1170	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	7	47	theme	whole	1409:1413	arg1	metabolites					1421:1431	whole plant metabolites	1409:1431	whole plant metabolites	1409:1431	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	2	48	from	data	373:376	arg1	composition					394:404	the chemical composition	381:404	the chemical composition of these Dracocephalum species	381:435	There are no data on the chemical composition of these Dracocephalum species.
36015428	3	49	theme	liquid	474:479	arg1	technique					514:522	a liquid chromatography-mass spectrometry technique	472:522	a liquid chromatography-mass spectrometry technique	472:522	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	4	50	theme	arabinogalactan-protein	908:930	arg1	complexes					932:940	additive arabinogalactan-protein complexes	899:940	additive arabinogalactan-protein complexes	899:940	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	5	51	theme	radical-scavenging	1115:1132	arg1	assays					1165:1170	radical-scavenging and ferrous (II) ion chelating assays	1115:1170	radical-scavenging and ferrous (II) ion chelating assays	1115:1170	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	7	52	theme	plant	1415:1419	arg1	metabolites					1421:1431	whole plant metabolites	1409:1431	whole plant metabolites	1409:1431	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	3	53	theme	chromatography-mass	481:499	arg1	technique					514:522	a liquid chromatography-mass spectrometry technique	472:522	a liquid chromatography-mass spectrometry technique	472:522	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	4	54	theme	D.	812:813	arg1	roots					803:807	roots	803:807	roots	803:807	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	4	54	theme	D.	812:813	arg1	herbs					793:797	herbs	793:797	herbs	793:797	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	1	55	theme	folk	327:330	arg1	medicine					332:339	folk medicine	327:339	folk medicine	327:339	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	4	56	theme	starch-like	946:956	arg1	compounds					958:966	starch-like compounds	946:966	starch-like compounds	946:966	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	6	57	theme	metabolites	1273:1283	arg1	roots					1212:1216	roots	1212:1216	roots	1212:1216	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	57	theme	metabolites	1273:1283	arg1	plants					1307:1312	valuable plants	1298:1312	valuable plants for new biologically active products	1298:1349	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	57	theme	metabolites	1273:1283	arg1	herbs					1202:1206	herbs	1202:1206	herbs	1202:1206	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	57	theme	metabolites	1273:1283	arg1	sources					1262:1268	rich sources	1257:1268	rich sources of metabolites	1257:1283	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	5	58	theme	selected	1040:1047	arg1	phenolics					1049:1057	selected phenolics	1040:1057	selected phenolics	1040:1057	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	7	59	from	activity	1455:1462	arg1	D.					1467:1468	D.	1467:1468	D.	1467:1468	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	7	59	from	activity	1455:1462	arg1	D.					1485:1486	D.	1485:1486	D.	1485:1486	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
36015428	1	60	theme	Lamiaceae	249:257	arg1	family					259:264	the Dracocephalum genus (Lamiaceae family)	224:265	the Dracocephalum genus (Lamiaceae family)	224:265	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	5	61	theme	ferrous	1138:1144	arg1	ion					1151:1153	ferrous (II) ion	1138:1153	ferrous (II) ion	1138:1153	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	4	62	theme	roots	803:807	arg1	pectins					886:892	mostly pectins	879:892	mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds	879:966	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	4	62	theme	roots	803:807	arg1	polysaccharides					770:784	Water-soluble polysaccharides	756:784	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides	756:842	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	5	63	theme	antioxidant	973:983	arg1	potential					985:993	The antioxidant potential	969:993	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides	969:1091	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	3	64	from	herbs	579:583	arg1	profiling					543:551	the metabolite profiling	528:551	the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides	528:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	64	from	herbs	579:583	arg1	extracts					565:572	methanol extracts	556:572	methanol extracts from herbs and roots of D. austriacum and D. botryoides	556:628	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	1	65	theme	family	259:264	arg1	species					213:219	species	213:219	species of the Dracocephalum genus (Lamiaceae family)	213:265	Dracocephalum botryoides Steven and Dracocephalum austriacum L. are unexplored species of the Dracocephalum genus (Lamiaceae family) with a distribution in the Caucasus, where they are used in folk medicine and local cuisine.
36015428	3	66	theme	compounds	667:675	arg1	identification					646:659	the identification	642:659	the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans	642:753	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	0	67	dep	Dragonheads	10:20	arg1	Polysaccharides					43:57	Polysaccharides	43:57	Polysaccharides	43:57	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	0	67	dep	Dragonheads	10:20	arg1	Bioactivity					64:74	Bioactivity	64:74	Bioactivity	64:74	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	0	67	dep	Dragonheads	10:20	arg1	Compounds					32:40	Phenolic Compounds	23:40	Phenolic Compounds	23:40	Caucasian Dragonheads: Phenolic Compounds, Polysaccharides, and Bioactivity of Dracocephalum austriacum and Dracocephalum botryoides.
36015428	5	68	theme	studied	1002:1008	arg1	extracts					1010:1017	the studied extracts	998:1017	the studied extracts of Dracocephalum and selected phenolics	998:1057	The antioxidant potential of the studied extracts of Dracocephalum and selected phenolics and water-soluble polysaccharides were investigated via radical-scavenging and ferrous (II) ion chelating assays.
36015428	6	69	theme	D.	1239:1240	arg1	roots					1212:1216	roots	1212:1216	roots	1212:1216	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	69	theme	D.	1239:1240	arg1	herbs					1202:1206	herbs	1202:1206	herbs	1202:1206	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	6	69	theme	D.	1239:1240	arg1	sources					1262:1268	rich sources	1257:1268	rich sources of metabolites	1257:1283	This paper demonstrates that herbs and roots of D. austriacum and D. botryoides are rich sources of metabolites and could be valuable plants for new biologically active products.
36015428	3	70	theme	D.	616:617	arg1	roots					589:593	roots	589:593	roots	589:593	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	3	70	theme	D.	616:617	arg1	herbs					579:583	herbs	579:583	herbs	579:583	In this study, the application of a liquid chromatography-mass spectrometry technique for the metabolite profiling of methanol extracts from herbs and roots of D. austriacum and D. botryoides resulted in the identification of 50 compounds, including benzoic acid derivatives, phenylpropanoids, flavonoids and lignans.
36015428	4	71	dep	herbs	793:797	arg1	the					789:791	the	789:791	the	789:791	Water-soluble polysaccharides of the herbs and roots of D. austriacum and D. botryoides were isolated and characterized as mostly pectins with additive arabinogalactan-protein complexes and starch-like compounds.
36015428	7	72	theme	antioxidant	1443:1453	arg1	activity					1455:1462	their antioxidant activity	1437:1462	their antioxidant activity in D. austriacum and D. botryoides	1437:1497	To the best of our knowledge, this is the first study of whole plant metabolites and their antioxidant activity in D. austriacum and D. botryoides.
35206093	7	0	theme	effective	1222:1230	arg1	polysaccharides					1188:1202	tea polysaccharides	1184:1202	tea polysaccharides	1184:1202	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	7	0	theme	effective	1222:1230	arg1	ingredient					1232:1241	an effective ingredient	1219:1241	an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats	1219:1327	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	6	1	theme	Oscillospira	1076:1087	arg1	treatment					1010:1018	treatment	1010:1018	treatment	1010:1018	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	1	theme	Oscillospira	1076:1087	arg1	blooms					1029:1034	blooms	1029:1034	blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira	1029:1087	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	1	theme	Oscillospira	1076:1087	arg1	reduction					1103:1111	a strong reduction	1094:1111	a strong reduction in Desulfovibrio and Lactobacillus	1094:1146	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	2	2	theme	1→	543:544	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	0	3	theme	Rock	74:77	arg1	Tea					79:81	Wuyi Rock Tea	69:81	Wuyi Rock Tea	69:81	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	2	4	theme	1→	485:486	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	4	5	dep	histopathology	774:787	arg1	analysis					806:813	analysis	806:813	analysis	806:813	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	3	6	theme	molecular	604:612	arg1	Da					638:639	3.9285 × 104 Da	625:639	3.9285 × 104 Da	625:639	The molecular weight was 3.9285 × 104 Da.
35206093	3	6	theme	molecular	604:612	arg1	weight					614:619	The molecular weight	600:619	The molecular weight	600:619	The molecular weight was 3.9285 × 104 Da.
35206093	4	7	theme	tea	676:678	arg1	polysaccharides					680:694	acidic tea polysaccharides	669:694	acidic tea polysaccharides	669:694	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	0	8	from	Characterization	0:15	arg1	Yellow					52:57	Yellow	52:57	Yellow	52:57	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	6	9	from	treatment	1010:1018	arg1	Lactobacillus					1134:1146	Lactobacillus	1134:1146	Lactobacillus	1134:1146	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	9	from	treatment	1010:1018	arg1	Desulfovibrio					1116:1128	Desulfovibrio	1116:1128	Desulfovibrio	1116:1128	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	4	10	theme	diabetes	729:736	arg1	mellitus					738:745	type 2 diabetes mellitus	722:745	streptozotocin-induced type 2 diabetes mellitus rats	699:750	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	2	11	theme	1→	447:448	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	12	theme	-α-l-Rhap-	474:483	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	6	13	theme	Dorea	1065:1069	arg1	treatment					1010:1018	treatment	1010:1018	treatment	1010:1018	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	13	theme	Dorea	1065:1069	arg1	blooms					1029:1034	blooms	1029:1034	blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira	1029:1087	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	13	theme	Dorea	1065:1069	arg1	reduction					1103:1111	a strong reduction	1094:1111	a strong reduction in Desulfovibrio and Lactobacillus	1094:1146	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	14	theme	flora	978:982	arg1	composition					948:958	the composition	944:958	the composition of the intestinal flora	944:982	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	15	theme	rRNA	909:912	arg1	sequencing					919:928	16S rRNA gene sequencing	905:928	16S rRNA gene sequencing	905:928	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	4	16	theme	mellitus	738:745	arg1	rats					747:750	streptozotocin-induced type 2 diabetes mellitus rats	699:750	streptozotocin-induced type 2 diabetes mellitus rats	699:750	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	0	17	from	Yellow	52:57	arg1	Characterization					0:15	Characterization	0:15	Characterization of Acidic Tea Polysaccharides from Yellow	0:57	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	0	17	from	Yellow	52:57	arg1	Polysaccharides					31:45	Acidic Tea Polysaccharides	20:45	Acidic Tea Polysaccharides from Yellow	20:57	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	7	18	theme	tea	1184:1186	arg1	polysaccharides					1188:1202	tea polysaccharides	1184:1202	tea polysaccharides	1184:1202	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	7	18	theme	tea	1184:1186	arg1	ingredient					1232:1241	an effective ingredient	1219:1241	an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats	1219:1327	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	2	19	theme	1→	505:506	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	6	20	theme	intestinal	967:976	arg1	flora					978:982	the intestinal flora	963:982	the intestinal flora	963:982	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	1	21	theme	bioactive	158:166	arg1	polysaccharide					179:192	A bioactive acidic tea polysaccharide	156:192	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea	156:228	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	0	22	theme	Hypoglycemic	93:104	arg1	Activity					106:113	Their Hypoglycemic Activity	87:113	Their Hypoglycemic Activity	87:113	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	2	23	theme	-α-d-Glcp-	494:503	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	6	24	theme	16S	905:907	arg1	sequencing					919:928	16S rRNA gene sequencing	905:928	16S rRNA gene sequencing	905:928	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	1	25	theme	acidic	168:173	arg1	polysaccharide					179:192	A bioactive acidic tea polysaccharide	156:192	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea	156:228	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	2	26	theme	-α-d-Glcp-	426:435	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	27	theme	1→	524:525	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	28	theme	1→	557:558	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	1	29	theme	tea	175:177	arg1	polysaccharide					179:192	A bioactive acidic tea polysaccharide	156:192	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea	156:228	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	2	30	theme	-α-l-Araf-	408:417	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	6	31	theme	Bifidobacterium	1039:1053	arg1	treatment					1010:1018	treatment	1010:1018	treatment	1010:1018	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	31	theme	Bifidobacterium	1039:1053	arg1	blooms					1029:1034	blooms	1029:1034	blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira	1029:1087	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	31	theme	Bifidobacterium	1039:1053	arg1	reduction					1103:1111	a strong reduction	1094:1111	a strong reduction in Desulfovibrio and Lactobacillus	1094:1146	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	7	32	from	metabolism	1265:1274	arg1	rats					1324:1327	hyperglycemic rats	1310:1327	hyperglycemic rats	1310:1327	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	2	33	theme	resonance	311:319	arg1	analysis					327:334	Nuclear magnetic resonance (NMR) analysis	294:334	Nuclear magnetic resonance (NMR) analysis	294:334	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	0	34	theme	Tea	27:29	arg1	Polysaccharides					31:45	Acidic Tea Polysaccharides	20:45	Acidic Tea Polysaccharides from Yellow	20:57	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	6	35	from	reduction	1103:1111	arg1	Lactobacillus					1134:1146	Lactobacillus	1134:1146	Lactobacillus	1134:1146	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	35	from	reduction	1103:1111	arg1	Desulfovibrio					1116:1128	Desulfovibrio	1116:1128	Desulfovibrio	1116:1128	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	0	36	theme	Intestinal	119:128	arg1	Flora					130:134	Intestinal Flora	119:134	Intestinal Flora Regulation in Rats	119:153	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	2	37	theme	-α-d-GalAp-	512:522	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	38	theme	magnetic	302:309	arg1	NMR					322:324	NMR	322:324	NMR	322:324	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	38	theme	magnetic	302:309	arg1	resonance					311:319	Nuclear magnetic resonance	294:319	Nuclear magnetic resonance (NMR) analysis	294:334	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	0	39	theme	Acidic	20:25	arg1	Polysaccharides					31:45	Acidic Tea Polysaccharides	20:45	Acidic Tea Polysaccharides from Yellow	20:57	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	7	40	theme	intestinal	1290:1299	arg1	flora					1301:1305	intestinal flora	1290:1305	intestinal flora in hyperglycemic rats	1290:1327	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	2	41	theme	-α-d-Glcp-	454:463	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	42	theme	Nuclear	294:300	arg1	NMR					322:324	NMR	322:324	NMR	322:324	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	42	theme	Nuclear	294:300	arg1	resonance					311:319	Nuclear magnetic resonance	294:319	Nuclear magnetic resonance (NMR) analysis	294:334	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	43	theme	1→	401:402	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	4	44	theme	streptozotocin-induced	699:720	arg1	rats					747:750	streptozotocin-induced type 2 diabetes mellitus rats	699:750	streptozotocin-induced type 2 diabetes mellitus rats	699:750	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	5	45	theme	liver	871:875	arg1	metabolism					883:892	plasma and liver lipid metabolism	860:892	plasma and liver lipid metabolism	860:892	The acidic tea polysaccharide could improve plasma and liver lipid metabolism.
35206093	0	46	theme	Polysaccharides	31:45	arg1	Characterization					0:15	Characterization	0:15	Characterization of Acidic Tea Polysaccharides from Yellow	0:57	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	0	47	theme	Flora	130:134	arg1	Regulation					136:145	Intestinal Flora Regulation	119:145	Intestinal Flora Regulation in Rats	119:153	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	5	48	theme	lipid	877:881	arg1	metabolism					883:892	plasma and liver lipid metabolism	860:892	plasma and liver lipid metabolism	860:892	The acidic tea polysaccharide could improve plasma and liver lipid metabolism.
35206093	7	49	theme	glucose	1257:1263	arg1	metabolism					1265:1274	glucose metabolism disorders and intestinal flora in hyperglycemic rats	1257:1327	metabolism	1265:1274	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	-β-d-Galp-					585:594	-β-d-Galp-	585:594	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→	391:597	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→5					405:406	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→5	405:406	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	α-d-Galp-					547:555	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	α-d-Galp-	547:555	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→4					509:510	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→4	509:510	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→2,4					469:472	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→2,4	469:472	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→4					528:529	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→4	528:529	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	1→					575:576	1→	575:576	1→	575:576	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	1→					596:597	1→	596:597	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→	391:597	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→6					451:452	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→6	451:452	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→3,4					489:492	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→3,4	489:492	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→6					561:562	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→6	561:562	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→4					582:583	→4	582:583	→4	582:583	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	→4					423:424	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	→4	423:424	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	50	dep	-β-d-Galp-	564:573	arg1	Arap-					441:445	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	Arap-	441:445	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	51	theme	α-l-Araf-	391:399	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	7	52	from	flora	1301:1305	arg1	rats					1324:1327	hyperglycemic rats	1310:1327	hyperglycemic rats	1310:1327	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	6	53	theme	strong	1096:1101	arg1	reduction					1103:1111	a strong reduction	1094:1111	a strong reduction in Desulfovibrio and Lactobacillus	1094:1146	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	5	54	theme	acidic	820:825	arg1	polysaccharide					831:844	The acidic tea polysaccharide	816:844	The acidic tea polysaccharide	816:844	The acidic tea polysaccharide could improve plasma and liver lipid metabolism.
35206093	2	55	theme	-α-d-GalAp-	531:541	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	2	56	theme	1→	419:420	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	7	57	dep	metabolism	1265:1274	arg1	disorders					1276:1284	disorders	1276:1284	disorders	1276:1284	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	4	58	theme	polysaccharides	680:694	arg1	effect					659:664	The hypoglycemic effect	642:664	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats	642:750	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	5	59	theme	tea	827:829	arg1	polysaccharide					831:844	The acidic tea polysaccharide	816:844	The acidic tea polysaccharide	816:844	The acidic tea polysaccharide could improve plasma and liver lipid metabolism.
35206093	3	60	theme	3.9285	625:630	arg1	Da					638:639	3.9285 × 104 Da	625:639	3.9285 × 104 Da	625:639	The molecular weight was 3.9285 × 104 Da.
35206093	3	60	theme	3.9285	625:630	arg1	weight					614:619	The molecular weight	600:619	The molecular weight	600:619	The molecular weight was 3.9285 × 104 Da.
35206093	4	61	theme	hypoglycemic	646:657	arg1	effect					659:664	The hypoglycemic effect	642:664	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats	642:750	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	4	62	theme	acidic	669:674	arg1	polysaccharides					680:694	acidic tea polysaccharides	669:694	acidic tea polysaccharides	669:694	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	7	63	theme	hyperglycemic	1310:1322	arg1	rats					1324:1327	hyperglycemic rats	1310:1327	hyperglycemic rats	1310:1327	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	1	64	from	yellow	199:204	arg1	polysaccharide					179:192	A bioactive acidic tea polysaccharide	156:192	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea	156:228	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	6	65	theme	gene	914:917	arg1	sequencing					919:928	16S rRNA gene sequencing	905:928	16S rRNA gene sequencing	905:928	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	3	66	theme	×	632:632	arg1	Da					638:639	3.9285 × 104 Da	625:639	3.9285 × 104 Da	625:639	The molecular weight was 3.9285 × 104 Da.
35206093	3	66	theme	×	632:632	arg1	weight					614:619	The molecular weight	600:619	The molecular weight	600:619	The molecular weight was 3.9285 × 104 Da.
35206093	0	67	from	Regulation	136:145	arg1	Rats					150:153	Rats	150:153	Rats	150:153	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	2	68	theme	1→	465:466	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	4	69	from	effect	659:664	arg1	rats					747:750	streptozotocin-induced type 2 diabetes mellitus rats	699:750	streptozotocin-induced type 2 diabetes mellitus rats	699:750	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35206093	7	70	theme	above	1153:1157	arg1	results					1159:1165	The above results	1149:1165	The above results	1149:1165	The above results illustrated that tea polysaccharides might serve as an effective ingredient to ameliorate glucose metabolism disorders and intestinal flora in hyperglycemic rats.
35206093	2	71	theme	main	352:355	arg1	bonds					368:372	the main glycosidic bonds	348:372	the main glycosidic bonds	348:372	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	1	72	theme	rock	221:224	arg1	tea					226:228	Wuyi rock tea	216:228	Wuyi rock tea	216:228	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	0	73	theme	Wuyi	69:72	arg1	Tea					79:81	Wuyi Rock Tea	69:81	Wuyi Rock Tea	69:81	Characterization of Acidic Tea Polysaccharides from Yellow Leaves of Wuyi Rock Tea and Their Hypoglycemic Activity via Intestinal Flora Regulation in Rats.
35206093	5	74	theme	plasma	860:865	arg1	metabolism					883:892	plasma and liver lipid metabolism	860:892	plasma and liver lipid metabolism	860:892	The acidic tea polysaccharide could improve plasma and liver lipid metabolism.
35206093	2	75	theme	glycosidic	357:366	arg1	bonds					368:372	the main glycosidic bonds	348:372	the main glycosidic bonds	348:372	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	6	76	from	blooms	1029:1034	arg1	Lactobacillus					1134:1146	Lactobacillus	1134:1146	Lactobacillus	1134:1146	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	6	76	from	blooms	1029:1034	arg1	Desulfovibrio					1116:1128	Desulfovibrio	1116:1128	Desulfovibrio	1116:1128	Moreover, 16S rRNA gene sequencing revealed that the composition of the intestinal flora changed drastically after treatment, namely, blooms of Bifidobacterium, Blautia, Dorea, and Oscillospira, and a strong reduction in Desulfovibrio and Lactobacillus.
35206093	1	77	theme	tea	226:228	arg1	yellow					199:204	yellow	199:204	yellow	199:204	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	2	78	theme	1→	437:438	arg1	-β-d-Galp-					564:573	α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-	391:573	-β-d-Galp-	564:573	Nuclear magnetic resonance (NMR) analysis showed that the main glycosidic bonds were composed of α-l-Araf-(1→, →5)-α-l-Araf-(1→, →4)-α-d-Glcp-(1→, Arap-(1→, →6)-α-d-Glcp-(1→, →2,4)-α-l-Rhap-(1→, →3,4)-α-d-Glcp-(1→, →4)-α-d-GalAp-(1→, →4)-α-d-GalAp-(1→, α-d-Galp-(1→, →6)-β-d-Galp-(1→ and →4)-β-d-Galp-(1→.
35206093	1	79	dep	yellow	199:204	arg1	leaves					206:211	leaves	206:211	leaves	206:211	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	1	80	theme	Wuyi	216:219	arg1	tea					226:228	Wuyi rock tea	216:228	Wuyi rock tea	216:228	A bioactive acidic tea polysaccharide from yellow leaves of Wuyi rock tea was successively prepared via DEAE-52 and Superdex-200 columns.
35206093	4	81	theme	type	722:725	arg1	mellitus					738:745	type 2 diabetes mellitus	722:745	streptozotocin-induced type 2 diabetes mellitus rats	699:750	The hypoglycemic effect of acidic tea polysaccharides on streptozotocin-induced type 2 diabetes mellitus rats was evaluated through histopathology and biochemistry analysis.
35432934	0	0	theme	wolf	71:74	arg1	astrigera					91:99	the wolf spider Pardosa astrigera	67:99	the wolf spider Pardosa astrigera	67:99	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
35432934	4	1	theme	male	479:482	arg1	astrigera					492:500	female and male Pardosa astrigera	468:500	female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing	468:544	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	7	2	dep	Actinobacteriota	802:817	arg1	belongs					836:842	belongs	836:842	belongs to Actinobacteriota	836:862	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	1	3	theme	factors	130:136	arg1	one					109:111	one	109:111	one	109:111	Sex is one of the important factors affecting gut microbiota.
35432934	1	3	theme	factors	130:136	arg1	factors					130:136	the important factors	116:136	the important factors affecting gut microbiota	116:161	Sex is one of the important factors affecting gut microbiota.
35432934	9	4	theme	distinct	1526:1533	arg1	differences					1535:1545	the distinct differences	1522:1545	the distinct differences of bacterial composition	1522:1570	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	4	theme	distinct	1526:1533	arg1	due					1583:1585	due	1583:1585	due	1583:1585	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	5	theme	gut	1467:1469	arg1	community					1481:1489	shaping gut bacterial community	1459:1489	shaping gut bacterial community in P. astrigera spiders	1459:1513	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	0	6	theme	Pardosa	83:89	arg1	astrigera					91:99	the wolf spider Pardosa astrigera	67:99	the wolf spider Pardosa astrigera	67:99	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
35432934	0	7	from	Divergence	0:9	arg1	community					28:36	gut bacterial community	14:36	gut bacterial community	14:36	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
35432934	5	8	theme	bacterial	583:591	arg1	microbiota					593:602	bacterial microbiota	583:602	bacterial microbiota	583:602	Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05).
35432934	2	9	theme	nutritional	276:286	arg1	predators					171:179	key predators	167:179	key predators in agroforestry ecosystem	167:205	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	9	theme	nutritional	276:286	arg1	habits					265:270	dramatically different activity habits	233:270	dramatically different activity habits	233:270	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	9	theme	nutritional	276:286	arg1	requirements					288:299	nutritional requirements	276:299	nutritional requirements	276:299	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	9	10	from	community	1481:1489	arg1	spiders					1507:1513	P. astrigera spiders	1494:1513	P. astrigera spiders	1494:1513	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	3	11	theme	gut	353:355	arg1	microbiota					357:366	gut microbiota	353:366	gut microbiota of spiders	353:377	However, how sex affects gut microbiota of spiders remains unclear.
35432934	5	12	theme	male	654:657	arg1	p < .05					668:674	p < .05	668:674	p < .05	668:674	Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05).
35432934	5	12	theme	male	654:657	arg1	spiders					659:665	male spiders	654:665	male spiders (p < .05)	654:675	Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05).
35432934	5	13	theme	microbiota	593:602	arg1	richness					571:578	the richness	567:578	the richness of bacterial microbiota in female spiders	567:620	Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05).
35432934	5	13	theme	microbiota	593:602	arg1	lower					640:644	lower	640:644	lower	640:644	Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05).
35432934	9	14	theme	shaping	1459:1465	arg1	community					1481:1489	shaping gut bacterial community	1459:1489	shaping gut bacterial community in P. astrigera spiders	1459:1513	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	0	15	from	females	46:52	arg1	astrigera					91:99	the wolf spider Pardosa astrigera	67:99	the wolf spider Pardosa astrigera	67:99	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
35432934	5	16	theme	female	607:612	arg1	spiders					614:620	female spiders	607:620	female spiders	607:620	Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05).
35432934	1	17	theme	gut	148:150	arg1	microbiota					152:161	gut microbiota	148:161	gut microbiota	148:161	Sex is one of the important factors affecting gut microbiota.
35432934	2	18	theme	activity	256:263	arg1	predators					171:179	key predators	167:179	key predators in agroforestry ecosystem	167:205	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	18	theme	activity	256:263	arg1	habits					265:270	dramatically different activity habits	233:270	dramatically different activity habits	233:270	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	18	theme	activity	256:263	arg1	requirements					288:299	nutritional requirements	276:299	nutritional requirements	276:299	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	3	19	theme	spiders	371:377	arg1	microbiota					357:366	gut microbiota	353:366	gut microbiota of spiders	353:377	However, how sex affects gut microbiota of spiders remains unclear.
35432934	4	20	theme	female	468:473	arg1	astrigera					492:500	female and male Pardosa astrigera	468:500	female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing	468:544	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	9	21	theme	energy	1622:1627	arg1	requirements					1629:1640	their different nutritional and energy requirements	1590:1640	requirements	1629:1640	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	22	theme	P.	1494:1495	arg1	spiders					1507:1513	P. astrigera spiders	1494:1513	P. astrigera spiders	1494:1513	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	23	theme	astrigera	1497:1505	arg1	spiders					1507:1513	P. astrigera spiders	1494:1513	P. astrigera spiders	1494:1513	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	4	24	theme	bacteria	451:458	arg1	diversity					434:442	diversity	434:442	diversity	434:442	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	4	24	theme	bacteria	451:458	arg1	composition					418:428	composition	418:428	composition	418:428	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	7	25	theme	relative	780:787	arg1	higher					883:888	higher	883:888	higher	883:888	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	25	theme	relative	780:787	arg1	abundance					789:797	The relative abundance	776:797	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota)	776:863	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	26	theme	male	908:911	arg1	p < .05					922:928	p < .05	922:928	p < .05	922:928	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	26	theme	male	908:911	arg1	spiders					913:919	male spiders	908:919	male spiders (p < .05)	908:929	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	8	27	theme	lipid	1199:1203	arg1	metabolisms					1205:1215	lipid metabolisms	1199:1215	lipid metabolisms	1199:1215	The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05).
35432934	0	28	theme	bacterial	18:26	arg1	community					28:36	gut bacterial community	14:36	gut bacterial community	14:36	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
35432934	4	29	theme	gene	530:533	arg1	sequencing					535:544	bacterial 16S rRNA gene sequencing	511:544	bacterial 16S rRNA gene sequencing	511:544	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	7	30	dep	higher	883:888	arg1	whereas					932:938	whereas	932:938	whereas	932:938	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	31	theme	Firmicutes	966:975	arg1	Fusicatenibacter					1044:1059	Fusicatenibacter	1044:1059	Fusicatenibacter	1044:1059	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	31	theme	Firmicutes	966:975	arg1	abundance					953:961	the relative abundance	940:961	the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria)	940:1021	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	31	theme	Firmicutes	966:975	arg1	Ruminococcus					1027:1038	Ruminococcus	1027:1038	Ruminococcus	1027:1038	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	31	theme	Firmicutes	966:975	arg1	higher					1106:1111	higher	1106:1111	higher	1106:1111	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	32	theme	Rhodococcus	823:833	arg1	higher					883:888	higher	883:888	higher	883:888	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	32	theme	Rhodococcus	823:833	arg1	abundance					789:797	The relative abundance	776:797	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota)	776:863	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	9	33	theme	bacterial	1550:1558	arg1	composition					1560:1570	bacterial composition	1550:1570	bacterial composition	1550:1570	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	0	34	theme	gut	14:16	arg1	community					28:36	gut bacterial community	14:36	gut bacterial community	14:36	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
35432934	7	35	dep	Firmicutes	966:975	arg1	belongs					996:1002	belongs	996:1002	belongs to Proteobacteria	996:1020	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	9	36	from	factor	1449:1454	arg1	community					1481:1489	shaping gut bacterial community	1459:1489	shaping gut bacterial community in P. astrigera spiders	1459:1513	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	2	37	theme	spider	213:218	arg1	species					220:226	many spider species	208:226	many spider species	208:226	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	7	38	theme	Actinobacteriota	802:817	arg1	higher					883:888	higher	883:888	higher	883:888	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	38	theme	Actinobacteriota	802:817	arg1	abundance					789:797	The relative abundance	776:797	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota)	776:863	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	4	39	theme	bacterial	511:519	arg1	sequencing					535:544	bacterial 16S rRNA gene sequencing	511:544	bacterial 16S rRNA gene sequencing	511:544	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	4	40	theme	gut	447:449	arg1	bacteria					451:458	gut bacteria	447:458	gut bacteria	447:458	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	2	41	theme	many	208:211	arg1	species					220:226	many spider species	208:226	many spider species	208:226	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	7	42	theme	female	1129:1134	arg1	spiders					1136:1142	female spiders	1129:1142	female spiders (p < .05)	1129:1152	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	42	theme	female	1129:1134	arg1	p < .05					1145:1151	p < .05	1145:1151	p < .05	1145:1151	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	8	43	theme	amino	1184:1188	arg1	acid					1190:1193	amino acid	1184:1193	amino acid	1184:1193	The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05).
35432934	2	44	from	predators	171:179	arg1	ecosystem					197:205	agroforestry ecosystem	184:205	agroforestry ecosystem	184:205	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	0	45	from	males	58:62	arg1	astrigera					91:99	the wolf spider Pardosa astrigera	67:99	the wolf spider Pardosa astrigera	67:99	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
35432934	4	46	theme	rRNA	525:528	arg1	sequencing					535:544	bacterial 16S rRNA gene sequencing	511:544	bacterial 16S rRNA gene sequencing	511:544	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	2	47	theme	agroforestry	184:195	arg1	ecosystem					197:205	agroforestry ecosystem	184:205	agroforestry ecosystem	184:205	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	5	48	from	richness	571:578	arg1	spiders					614:620	female spiders	607:620	female spiders	607:620	Results showed that the richness of bacterial microbiota in female spiders was significantly lower than in male spiders (p < .05).
35432934	4	49	theme	16S	521:523	arg1	sequencing					535:544	bacterial 16S rRNA gene sequencing	511:544	bacterial 16S rRNA gene sequencing	511:544	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	8	50	theme	female	1370:1375	arg1	p < .05					1386:1392	p < .05	1386:1392	p < .05	1386:1392	The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05).
35432934	8	50	theme	female	1370:1375	arg1	spiders					1377:1383	female spiders	1370:1383	female spiders (p < .05)	1370:1393	The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05).
35432934	2	51	theme	different	246:254	arg1	predators					171:179	key predators	167:179	key predators in agroforestry ecosystem	167:205	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	51	theme	different	246:254	arg1	habits					265:270	dramatically different activity habits	233:270	dramatically different activity habits	233:270	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	51	theme	different	246:254	arg1	requirements					288:299	nutritional requirements	276:299	nutritional requirements	276:299	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	7	52	theme	Acinetobacter	981:993	arg1	Fusicatenibacter					1044:1059	Fusicatenibacter	1044:1059	Fusicatenibacter	1044:1059	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	52	theme	Acinetobacter	981:993	arg1	abundance					953:961	the relative abundance	940:961	the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria)	940:1021	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	52	theme	Acinetobacter	981:993	arg1	Ruminococcus					1027:1038	Ruminococcus	1027:1038	Ruminococcus	1027:1038	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	52	theme	Acinetobacter	981:993	arg1	higher					1106:1111	higher	1106:1111	higher	1106:1111	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	8	53	theme	male	1261:1264	arg1	p < .05					1275:1281	p < .05	1275:1281	p < .05	1275:1281	The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05).
35432934	8	53	theme	male	1261:1264	arg1	spiders					1266:1272	male spiders	1261:1272	male spiders (p < .05)	1261:1282	The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05).
35432934	4	54	dep	composition	418:428	arg1	the					414:416	the	414:416	the	414:416	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	8	55	theme	glycan	1293:1298	arg1	biosynthesis					1300:1311	glycan biosynthesis	1293:1311	glycan biosynthesis	1293:1311	The results also showed that amino acid and lipid metabolisms were significantly higher in female than in male spiders (p < .05), whereas glycan biosynthesis and metabolism were significantly higher in male than in female spiders (p < .05).
35432934	9	56	theme	bacterial	1471:1479	arg1	community					1481:1489	shaping gut bacterial community	1459:1489	shaping gut bacterial community in P. astrigera spiders	1459:1513	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	57	theme	composition	1560:1570	arg1	differences					1535:1545	the distinct differences	1522:1545	the distinct differences of bacterial composition	1522:1570	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	57	theme	composition	1560:1570	arg1	due					1583:1585	due	1583:1585	due	1583:1585	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	58	theme	crucial	1441:1447	arg1	variation					1426:1434	sexual variation	1419:1434	sexual variation	1419:1434	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	58	theme	crucial	1441:1447	arg1	factor					1449:1454	a crucial factor	1439:1454	a crucial factor	1439:1454	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	7	59	dep	Ruminococcus	1027:1038	arg1	belong					1066:1071	belong	1066:1071	belong to Firmicutes	1066:1085	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	2	60	theme	key	167:169	arg1	predators					171:179	key predators	167:179	key predators in agroforestry ecosystem	167:205	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	60	theme	key	167:169	arg1	habits					265:270	dramatically different activity habits	233:270	dramatically different activity habits	233:270	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	2	60	theme	key	167:169	arg1	requirements					288:299	nutritional requirements	276:299	nutritional requirements	276:299	As key predators in agroforestry ecosystem, many spider species show dramatically different activity habits and nutritional requirements between females and males.
35432934	6	61	theme	significant	708:718	arg1	difference					720:729	a significant difference	706:729	a significant difference between female and male spiders (p = .0270)	706:773	Besides, β-diversity showed a significant difference between female and male spiders (p = .0270).
35432934	9	62	theme	sexual	1419:1424	arg1	variation					1426:1434	sexual variation	1419:1434	sexual variation	1419:1434	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	9	62	theme	sexual	1419:1424	arg1	factor					1449:1454	a crucial factor	1439:1454	a crucial factor	1439:1454	Our results imply that sexual variation is a crucial factor in shaping gut bacterial community in P. astrigera spiders, while the distinct differences of bacterial composition are mainly due to their different nutritional and energy requirements.
35432934	4	63	theme	Pardosa	484:490	arg1	astrigera					492:500	female and male Pardosa astrigera	468:500	female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing	468:544	Here, we compared the composition and diversity of gut bacteria between female and male Pardosa astrigera based on bacterial 16S rRNA gene sequencing.
35432934	7	64	theme	relative	944:951	arg1	abundance					953:961	the relative abundance	940:961	the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria)	940:1021	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	7	64	theme	relative	944:951	arg1	higher					1106:1111	higher	1106:1111	higher	1106:1111	The relative abundance of Actinobacteriota and Rhodococcus (belongs to Actinobacteriota) was significantly higher in female than in male spiders (p < .05), whereas the relative abundance of Firmicutes and Acinetobacter (belongs to Proteobacteria) and Ruminococcus and Fusicatenibacter (all belong to Firmicutes) was significantly higher in male than in female spiders (p < .05).
35432934	1	65	theme	important	120:128	arg1	factors					130:136	the important factors	116:136	the important factors affecting gut microbiota	116:161	Sex is one of the important factors affecting gut microbiota.
35432934	6	66	dep	female	739:744	arg1	spiders					755:761	spiders	755:761	spiders	755:761	Besides, β-diversity showed a significant difference between female and male spiders (p = .0270).
35432934	0	67	theme	spider	76:81	arg1	astrigera					91:99	the wolf spider Pardosa astrigera	67:99	the wolf spider Pardosa astrigera	67:99	Divergence in gut bacterial community between females and males in the wolf spider Pardosa astrigera.
37299211	5	0	with	spectroscopy	854:865	arg1	Fourier					872:878	Fourier	872:878	Fourier transform	872:888	The chemical structure was studied by infrared spectroscopy with Fourier transform (FTIR).
37299211	4	1	from	decrease	654:661	arg1	pH.					666:668	pH.	666:668	pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM)	666:804	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	3	2	theme	low	486:488	arg1	density					490:496	a low density	484:496	a low density of spongy material	484:515	The compression test showed a low density of spongy material.
37299211	8	3	theme	mechanical	1416:1425	arg1	results					1439:1445	the mechanical and thermal results	1412:1445	the mechanical and thermal results obtained	1412:1454	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	4	4	theme	compressive	540:550	arg1	strength					552:559	the compressive strength	536:559	the compressive strength	536:559	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	0	5	from	Development	0:10	arg1	Brazil					77:82	Brazil	77:82	Brazil	77:82	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	5	from	Development	0:10	arg1	Piauí					70:74	Piauí	70:74	Piauí	70:74	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	5	from	Development	0:10	arg1	Clay					60:63	Clay	60:63	Clay	60:63	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	5	from	Development	0:10	arg1	Polysaccharides					88:102	Polysaccharides	88:102	Polysaccharides	88:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	5	6	theme	infrared	845:852	arg1	spectroscopy					854:865	infrared spectroscopy	845:865	infrared spectroscopy with Fourier transform (FTIR)	845:895	The chemical structure was studied by infrared spectroscopy with Fourier transform (FTIR).
37299211	5	6	theme	infrared	845:852	arg1	FTIR					891:894	FTIR	891:894	FTIR	891:894	The chemical structure was studied by infrared spectroscopy with Fourier transform (FTIR).
37299211	4	7	dep	pH.	666:668	arg1	investigated					727:738	investigated	727:738	were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM)	722:804	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	6	8	contain	had	969:971	arg2	loss					980:983	a mass loss	973:983	a mass loss of 9%	973:989	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	6	8	contain	had	969:971	arg1	clay					964:967	the clay	960:967	the clay	960:967	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	1	9	theme	aerogels	256:263	arg1	properties					238:247	the physical and thermal properties	213:247	the physical and thermal properties of the aerogels	213:263	The incorporation of polymeric components into aerogels based on clay produces a significant improvement in the physical and thermal properties of the aerogels.
37299211	8	10	theme	thermal	1431:1437	arg1	results					1439:1445	the mechanical and thermal results	1412:1445	the mechanical and thermal results obtained	1412:1454	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	4	11	theme	elasticity	588:597	arg1	modulus					577:583	the Young's modulus	565:583	the Young's modulus	565:583	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	4	11	theme	elasticity	588:597	arg1	strength					552:559	the compressive strength	536:559	the compressive strength	536:559	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	3	12	theme	spongy	501:506	arg1	material					508:515	spongy material	501:515	spongy material	501:515	The compression test showed a low density of spongy material.
37299211	2	13	theme	sodium	367:372	arg1	alginate					374:381	sodium alginate	367:381	sodium alginate	367:381	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	6	14	from	atmosphere	934:943	arg1	curves					906:911	The TGA curves	898:911	The TGA curves from a non-oxidizing atmosphere	898:943	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	3	15	theme	material	508:515	arg1	density					490:496	a low density	484:496	a low density of spongy material	484:515	The compression test showed a low density of spongy material.
37299211	2	16	theme	mixing	423:428	arg1	method					430:435	a simple, ecologically acceptable mixing method	389:435	a simple, ecologically acceptable mixing method	389:435	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	8	17	theme	clay	1268:1271	arg1	aerogels					1251:1258	aerogels	1251:1258	aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied,	1251:1349	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	2	18	theme	gum	359:361	arg1	incorporation					335:347	incorporation	335:347	incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying	335:453	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	6	19	theme	mass	975:978	arg1	loss					980:983	a mass loss	973:983	a mass loss of 9%	973:989	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	6	20	theme	non-oxidizing	920:932	arg1	atmosphere					934:943	a non-oxidizing atmosphere	918:943	a non-oxidizing atmosphere	918:943	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	4	21	theme	microstructural	674:688	arg1	characteristics					690:704	The microstructural characteristics	670:704	The microstructural characteristics of the aerogels	670:720	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	5	22	theme	chemical	811:818	arg1	structure					820:828	The chemical structure	807:828	The chemical structure	807:828	The chemical structure was studied by infrared spectroscopy with Fourier transform (FTIR).
37299211	8	23	theme	ball	1263:1266	arg1	clay					1268:1271	ball clay	1263:1271	ball clay	1263:1271	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	2	24	theme	angico	352:357	arg1	gum					359:361	angico gum	352:361	angico gum	352:361	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	0	25	theme	New	17:19	arg1	Composite					40:48	a New Clay-Based Aerogel Composite	15:48	a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides	15:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	7	26	from	displacement	1175:1186	arg1	temperatures					1198:1209	higher temperatures	1191:1209	higher temperatures	1191:1209	The DSC curves of the aerogels demonstrated a displacement in higher temperatures.
37299211	2	27	theme	clay-based	281:290	arg1	aerogels					292:299	clay-based aerogels	281:299	clay-based aerogels	281:299	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	2	28	theme	simple	391:396	arg1	method					430:435	a simple, ecologically acceptable mixing method	389:435	a simple, ecologically acceptable mixing method	389:435	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	5	29	dep	Fourier	872:878	arg1	transform					880:888	transform	880:888	transform	880:888	The chemical structure was studied by infrared spectroscopy with Fourier transform (FTIR).
37299211	0	30	from	Piauí	70:74	arg1	Development					0:10	Development	0:10	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.	0:103	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	30	from	Piauí	70:74	arg1	Composite					40:48	a New Clay-Based Aerogel Composite	15:48	a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides	15:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	4	31	theme	aerogels	713:720	arg1	characteristics					690:704	The microstructural characteristics	670:704	The microstructural characteristics of the aerogels	670:720	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	1	32	theme	significant	186:196	arg1	improvement					198:208	a significant improvement	184:208	a significant improvement in the physical and thermal properties of the aerogels	184:263	The incorporation of polymeric components into aerogels based on clay produces a significant improvement in the physical and thermal properties of the aerogels.
37299211	0	33	theme	Aerogel	32:38	arg1	Composite					40:48	a New Clay-Based Aerogel Composite	15:48	a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides	15:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	7	34	theme	higher	1191:1196	arg1	temperatures					1198:1209	higher temperatures	1191:1209	higher temperatures	1191:1209	The DSC curves of the aerogels demonstrated a displacement in higher temperatures.
37299211	4	35	theme	electron	780:787	arg1	SEM					801:803	SEM	801:803	SEM	801:803	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	4	35	theme	electron	780:787	arg1	microscopy					789:798	scanning electron microscopy	771:798	scanning electron microscopy (SEM)	771:804	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	4	36	theme	aerogels	606:613	arg1	elasticity					588:597	elasticity	588:597	elasticity of the aerogels	588:613	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	0	37	theme	Clay-Based	21:30	arg1	Composite					40:48	a New Clay-Based Aerogel Composite	15:48	a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides	15:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	6	38	theme	TGA	902:904	arg1	curves					906:911	The TGA curves	898:911	The TGA curves from a non-oxidizing atmosphere	898:943	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	8	39	with	aerogels	1251:1258	arg1	incorporation					1282:1294	the incorporation	1278:1294	the incorporation of polysaccharides, which are still minimally studied,	1278:1349	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	0	40	from	Clay	60:63	arg1	Development					0:10	Development	0:10	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.	0:103	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	40	from	Clay	60:63	arg1	Composite					40:48	a New Clay-Based Aerogel Composite	15:48	a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides	15:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	4	41	theme	scanning	771:778	arg1	SEM					801:803	SEM	801:803	SEM	801:803	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	4	41	theme	scanning	771:778	arg1	microscopy					789:798	scanning electron microscopy	771:798	scanning electron microscopy (SEM)	771:804	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	1	42	from	improvement	198:208	arg1	properties					238:247	the physical and thermal properties	213:247	the physical and thermal properties of the aerogels	213:263	The incorporation of polymeric components into aerogels based on clay produces a significant improvement in the physical and thermal properties of the aerogels.
37299211	2	43	theme	alginate	374:381	arg1	incorporation					335:347	incorporation	335:347	incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying	335:453	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	2	44	dep	simple	391:396	arg1	acceptable					412:421	acceptable	412:421	acceptable	412:421	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	2	45	theme	ball	322:325	arg1	clay					327:330	a ball clay	320:330	a ball clay	320:330	In this study, clay-based aerogels were produced from a ball clay by incorporation of angico gum and sodium alginate using a simple, ecologically acceptable mixing method and freeze-drying.
37299211	0	46	theme	Composite	40:48	arg1	Development					0:10	Development	0:10	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.	0:103	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	47	from	Polysaccharides	88:102	arg1	Development					0:10	Development	0:10	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.	0:103	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	47	from	Polysaccharides	88:102	arg1	Composite					40:48	a New Clay-Based Aerogel Composite	15:48	a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides	15:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	8	48	theme	polysaccharides	1299:1313	arg1	incorporation					1282:1294	the incorporation	1278:1294	the incorporation of polysaccharides, which are still minimally studied,	1278:1349	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	6	49	theme	polysaccharides	1036:1050	arg1	presence					1024:1031	the presence	1020:1031	the presence of polysaccharides	1020:1050	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	7	50	theme	aerogels	1151:1158	arg1	curves					1137:1142	The DSC curves	1129:1142	The DSC curves of the aerogels	1129:1158	The DSC curves of the aerogels demonstrated a displacement in higher temperatures.
37299211	6	51	attach	presented	1066:1074	arg1	temperatures					1102:1113	temperatures	1102:1113	temperatures above 260 °C	1102:1126	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	6	51	attach	presented	1066:1074	arg2	aerogels					1057:1064	the aerogels	1053:1064	the aerogels	1053:1064	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	4	52	theme	X-ray	743:747	arg1	XRD					762:764	XRD	762:764	XRD	762:764	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	4	52	theme	X-ray	743:747	arg1	diffraction					749:759	X-ray diffraction	743:759	X-ray diffraction (XRD)	743:765	In addition, both the compressive strength and the Young's modulus of elasticity of the aerogels showed a progression associated to the decrease in pH. The microstructural characteristics of the aerogels were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM).
37299211	0	53	from	Brazil	77:82	arg1	Development					0:10	Development	0:10	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.	0:103	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	0	53	from	Brazil	77:82	arg1	Composite					40:48	a New Clay-Based Aerogel Composite	15:48	a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides	15:102	Development of a New Clay-Based Aerogel Composite from Ball Clay from Piauí, Brazil and Polysaccharides.
37299211	6	54	theme	%	1097:1097	arg1	decomposition					1078:1090	a decomposition	1076:1090	a decomposition of 20%	1076:1097	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	3	55	theme	compression	460:470	arg1	test					472:475	The compression test	456:475	The compression test	456:475	The compression test showed a low density of spongy material.
37299211	1	56	theme	physical	217:224	arg1	properties					238:247	the physical and thermal properties	213:247	the physical and thermal properties of the aerogels	213:263	The incorporation of polymeric components into aerogels based on clay produces a significant improvement in the physical and thermal properties of the aerogels.
37299211	8	57	theme	potential	1356:1364	arg1	application					1366:1376	potential application	1356:1376	potential application	1356:1376	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	8	58	theme	thermal	1381:1387	arg1	insulation					1389:1398	thermal insulation	1381:1398	thermal insulation considering the mechanical and thermal results obtained	1381:1454	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	6	59	theme	%	989:989	arg1	loss					980:983	a mass loss	973:983	a mass loss of 9%	973:989	The TGA curves from a non-oxidizing atmosphere indicated that the clay had a mass loss of 9% above 500 °C and that due to the presence of polysaccharides, the aerogels presented a decomposition of 20% at temperatures above 260 °C.
37299211	1	60	theme	polymeric	126:134	arg1	components					136:145	polymeric components	126:145	polymeric components	126:145	The incorporation of polymeric components into aerogels based on clay produces a significant improvement in the physical and thermal properties of the aerogels.
37299211	7	61	theme	DSC	1133:1135	arg1	curves					1137:1142	The DSC curves	1129:1142	The DSC curves of the aerogels	1129:1158	The DSC curves of the aerogels demonstrated a displacement in higher temperatures.
37299211	1	62	theme	components	136:145	arg1	incorporation					109:121	The incorporation	105:121	The incorporation of polymeric components into aerogels based on clay	105:173	The incorporation of polymeric components into aerogels based on clay produces a significant improvement in the physical and thermal properties of the aerogels.
37299211	8	63	contain	have	1351:1354	arg1	aerogels					1251:1258	aerogels	1251:1258	aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied,	1251:1349	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	8	63	contain	have	1351:1354	arg2	application					1366:1376	potential application	1356:1376	potential application	1356:1376	In conclusion, the results showed that aerogels of ball clay with the incorporation of polysaccharides, which are still minimally studied, have potential application as thermal insulation considering the mechanical and thermal results obtained.
37299211	1	64	theme	thermal	230:236	arg1	properties					238:247	the physical and thermal properties	213:247	the physical and thermal properties of the aerogels	213:263	The incorporation of polymeric components into aerogels based on clay produces a significant improvement in the physical and thermal properties of the aerogels.
36995114	0	0	theme	Glycosylation	99:111	arg1	Depletion					86:94	the Depletion	82:94	the Depletion of Glycosylation	82:111	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	6	1	theme	O-linked	1388:1395	arg1	glycosylation					1397:1409	O-linked glycosylation	1388:1409	O-linked glycosylation	1388:1409	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	6	2	theme	glycosylation	1397:1409	arg1	modulation					1374:1383	the modulation	1370:1383	the modulation of O-linked glycosylation with reductions up to 90%	1370:1435	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	1	3	theme	O-linked	168:175	arg1	glycosylation					185:197	O-linked protein glycosylation	168:197	O-linked protein glycosylation	168:197	The process of O-linked protein glycosylation is highly conserved across the Burkholderia genus and mediated by the oligosaccharyltransferase PglL.
36995114	1	4	theme	oligosaccharyltransferase	269:293	arg1	PglL					295:298	the oligosaccharyltransferase PglL	265:298	the oligosaccharyltransferase PglL	265:298	The process of O-linked protein glycosylation is highly conserved across the Burkholderia genus and mediated by the oligosaccharyltransferase PglL.
36995114	3	5	dep	diffusa	660:666	arg1	E264					744:747	E264	744:747	E264	744:747	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	1	6	theme	protein	177:183	arg1	glycosylation					185:197	O-linked protein glycosylation	168:197	O-linked protein glycosylation	168:197	The process of O-linked protein glycosylation is highly conserved across the Burkholderia genus and mediated by the oligosaccharyltransferase PglL.
36995114	6	7	dep	90	1433:1434	arg1	to					1430:1431	to	1430:1431	to	1430:1431	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	4	8	theme	glycosylation	1044:1056	arg1	inhibition					1030:1039	inhibition	1030:1039	inhibition of glycosylation by nearly 90%	1030:1070	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	6	9	theme	robust	1512:1517	arg1	tolerance					1519:1527	a robust tolerance	1510:1527	a robust tolerance to fluctuations in glycosylation capacity	1510:1569	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	5	10	theme	CRISPRi	1119:1125	arg1	induction					1127:1135	CRISPRi induction	1119:1135	CRISPRi induction with high levels of rhamnose	1119:1164	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	2	11	theme	Burkholderia	328:339	arg1	glycoproteomes					341:354	Burkholderia glycoproteomes	328:354	Burkholderia glycoproteomes	328:354	While our understanding of Burkholderia glycoproteomes has increased in recent years, little is known about how Burkholderia species respond to modulations in glycosylation.
36995114	6	12	theme	glycosylation	1548:1560	arg1	capacity					1562:1569	glycosylation capacity	1548:1569	glycosylation capacity	1548:1569	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	5	13	with	induction	1127:1135	arg1	levels					1147:1152	high levels	1142:1152	high levels of rhamnose	1142:1164	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	4	14	from	alterations	945:955	arg1	motility					960:967	motility	960:967	motility	960:967	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	3	15	theme	silencing	542:550	arg1	impact					532:537	the impact	528:537	the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264	528:747	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	0	16	theme	Modest	128:133	arg1	Impacts					144:150	Modest Proteome Impacts	128:150	Modest Proteome Impacts	128:150	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	6	17	from	fluctuations	1532:1543	arg1	capacity					1562:1569	glycosylation capacity	1548:1569	glycosylation capacity	1548:1569	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	1	18	theme	glycosylation	185:197	arg1	process					157:163	The process	153:163	The process of O-linked protein glycosylation	153:197	The process of O-linked protein glycosylation is highly conserved across the Burkholderia genus and mediated by the oligosaccharyltransferase PglL.
36995114	6	19	dep	%	1435:1435	arg1	90					1433:1434	90	1433:1434	90	1433:1434	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	4	20	from	changes	934:940	arg1	motility					960:967	motility	960:967	motility	960:967	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	4	21	theme	proteome	925:932	arg1	changes					934:940	proteome changes	925:940	proteome changes	925:940	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	6	22	link	O-linked	1388:1395	arg1	glycosylation					1397:1409	O-linked glycosylation	1388:1409	O-linked glycosylation	1388:1409	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	6	23	theme	phenotypic	1442:1451	arg1	levels					1466:1471	a phenotypic and proteome levels	1440:1471	a phenotypic and proteome levels	1440:1471	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	5	24	theme	Burkholderia	1200:1211	arg1	proteomes					1213:1221	the Burkholderia proteomes	1196:1221	the Burkholderia proteomes	1196:1221	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	3	25	theme	Burkholderia	615:626	arg1	K56-2					640:644	Burkholderia; Burkholderia cenocepacia K56-2	601:644	Burkholderia; Burkholderia cenocepacia K56-2	601:644	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	4	26	theme	Proteomic	750:758	arg1	analyses					779:786	Proteomic and glycoproteomic analyses	750:786	Proteomic and glycoproteomic analyses	750:786	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	0	27	theme	CRISPRi-Mediated	0:15	arg1	Silencing					17:25	CRISPRi-Mediated Silencing	0:25	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems	0:72	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	5	28	theme	CRISPRi	1299:1305	arg1	guides					1307:1312	CRISPRi guides	1299:1312	CRISPRi guides	1299:1312	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	3	29	theme	cenocepacia	628:638	arg1	K56-2					640:644	Burkholderia; Burkholderia cenocepacia K56-2	601:644	Burkholderia; Burkholderia cenocepacia K56-2	601:644	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	2	30	theme	Burkholderia	413:424	arg1	species					426:432	Burkholderia species	413:432	Burkholderia species	413:432	While our understanding of Burkholderia glycoproteomes has increased in recent years, little is known about how Burkholderia species respond to modulations in glycosylation.
36995114	0	31	theme	Burkholderia	30:41	arg1	Systems					66:72	Burkholderia O-Linked Glycosylation Systems	30:72	Burkholderia O-Linked Glycosylation Systems	30:72	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	1	32	link	O-linked	168:175	arg1	glycosylation					185:197	O-linked protein glycosylation	168:197	O-linked protein glycosylation	168:197	The process of O-linked protein glycosylation is highly conserved across the Burkholderia genus and mediated by the oligosaccharyltransferase PglL.
36995114	3	33	theme	Burkholderia	601:612	arg1	K56-2					640:644	Burkholderia; Burkholderia cenocepacia K56-2	601:644	Burkholderia; Burkholderia cenocepacia K56-2	601:644	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	4	34	theme	inducible	824:832	arg1	silencing					834:842	inducible silencing	824:842	inducible silencing of PglL	824:850	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	0	35	theme	Proteome	135:142	arg1	Impacts					144:150	Modest Proteome Impacts	128:150	Modest Proteome Impacts	128:150	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	5	36	theme	rhamnose	1157:1164	arg1	levels					1147:1152	high levels	1142:1152	high levels of rhamnose	1142:1164	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	2	37	theme	glycoproteomes	341:354	arg1	understanding					311:323	our understanding	307:323	our understanding of Burkholderia glycoproteomes	307:354	While our understanding of Burkholderia glycoproteomes has increased in recent years, little is known about how Burkholderia species respond to modulations in glycosylation.
36995114	4	38	theme	glycoproteomic	764:777	arg1	analyses					779:786	Proteomic and glycoproteomic analyses	750:786	Proteomic and glycoproteomic analyses	750:786	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	0	39	theme	Glycosylation	52:64	arg1	Systems					66:72	Burkholderia O-Linked Glycosylation Systems	30:72	Burkholderia O-Linked Glycosylation Systems	30:72	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	3	40	link	O-linked	555:562	arg1	glycosylation					564:576	O-linked glycosylation	555:576	O-linked glycosylation	555:576	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	3	41	theme	CRISPR	485:490	arg1	interference					492:503	CRISPR interference	485:503	CRISPR interference (CRISPRi)	485:513	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	3	41	theme	CRISPR	485:490	arg1	CRISPRi					506:512	CRISPRi	506:512	CRISPRi	506:512	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	1	42	theme	Burkholderia	230:241	arg1	genus					243:247	the Burkholderia genus	226:247	the Burkholderia genus	226:247	The process of O-linked protein glycosylation is highly conserved across the Burkholderia genus and mediated by the oligosaccharyltransferase PglL.
36995114	0	43	theme	O-Linked	43:50	arg1	Systems					66:72	Burkholderia O-Linked Glycosylation Systems	30:72	Burkholderia O-Linked Glycosylation Systems	30:72	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	5	44	theme	appropriate	1238:1248	arg1	controls					1250:1257	appropriate controls	1238:1257	appropriate controls	1238:1257	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	5	45	from	impacts	1185:1191	arg1	proteomes					1213:1221	the Burkholderia proteomes	1196:1221	the Burkholderia proteomes	1196:1221	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	6	46	theme	proteome	1457:1464	arg1	levels					1466:1471	a phenotypic and proteome levels	1440:1471	a phenotypic and proteome levels	1440:1471	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	5	47	theme	extensive	1175:1183	arg1	impacts					1185:1191	extensive impacts	1175:1191	extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides	1175:1312	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	3	48	theme	K56-2	640:644	arg1	species					590:596	four species	585:596	four species of Burkholderia; Burkholderia cenocepacia K56-2	585:644	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	3	48	theme	K56-2	640:644	arg1	diffusa					660:666	Burkholderia diffusa	647:666	Burkholderia diffusa MSMB375	647:674	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	3	48	theme	K56-2	640:644	arg1	thailandensis					730:742	Burkholderia thailandensis	717:742	Burkholderia thailandensis	717:742	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	3	48	theme	K56-2	640:644	arg1	multivorans					690:700	Burkholderia multivorans	677:700	Burkholderia multivorans ATCC17616	677:710	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	0	49	theme	Systems	66:72	arg1	Silencing					17:25	CRISPRi-Mediated Silencing	0:25	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems	0:72	CRISPRi-Mediated Silencing of Burkholderia O-Linked Glycosylation Systems Enables the Depletion of Glycosylation Yet Results in Modest Proteome Impacts.
36995114	6	50	with	modulation	1374:1383	arg1	reductions					1416:1425	reductions	1416:1425	reductions up to 90%	1416:1435	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	4	51	theme	null	1008:1011	arg1	strains					1013:1019	glycosylation null strains	994:1019	glycosylation null strains	994:1019	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	5	52	theme	high	1142:1145	arg1	levels					1147:1152	high levels	1142:1152	high levels of rhamnose	1142:1164	Importantly, this work also demonstrated that CRISPRi induction with high levels of rhamnose leads to extensive impacts on the Burkholderia proteomes, which without appropriate controls mask the impacts specifically driven by CRISPRi guides.
36995114	4	53	theme	PglL	847:850	arg1	silencing					834:842	inducible silencing	824:842	inducible silencing of PglL	824:850	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	4	54	theme	glycosylation	994:1006	arg1	strains					1013:1019	glycosylation null strains	994:1019	glycosylation null strains	994:1019	Proteomic and glycoproteomic analyses revealed that while CRISPRi enabled inducible silencing of PglL, this did not abolish glycosylation, nor recapitulate phenotypes such as proteome changes or alterations in motility that are associated with glycosylation null strains, despite inhibition of glycosylation by nearly 90%.
36995114	2	55	theme	recent	373:378	arg1	years					380:384	recent years	373:384	recent years	373:384	While our understanding of Burkholderia glycoproteomes has increased in recent years, little is known about how Burkholderia species respond to modulations in glycosylation.
36995114	2	56	from	modulations	445:455	arg1	glycosylation					460:472	glycosylation	460:472	glycosylation	460:472	While our understanding of Burkholderia glycoproteomes has increased in recent years, little is known about how Burkholderia species respond to modulations in glycosylation.
36995114	3	57	theme	O-linked	555:562	arg1	glycosylation					564:576	O-linked glycosylation	555:576	O-linked glycosylation	555:576	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
36995114	6	58	dep	reductions	1416:1425	arg1	%					1435:1435	%	1435:1435	%	1435:1435	Combined, this work revealed that while CRISPRi allows the modulation of O-linked glycosylation with reductions up to 90% at a phenotypic and proteome levels, Burkholderia appears to demonstrate a robust tolerance to fluctuations in glycosylation capacity.
36995114	3	59	theme	glycosylation	564:576	arg1	silencing					542:550	silencing	542:550	silencing of O-linked glycosylation	542:576	Utilizing CRISPR interference (CRISPRi), we explored the impact of silencing of O-linked glycosylation across four species of Burkholderia; Burkholderia cenocepacia K56-2, Burkholderia diffusa MSMB375, Burkholderia multivorans ATCC17616, and Burkholderia thailandensis E264.
37367617	3	0	theme	glucuronic	486:495	arg1	acid					497:500	glucuronic acid	486:500	glucuronic acid	486:500	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	2	1	from	use	288:290	arg1	heimuer					336:342	Auricularia heimuer	324:342	Auricularia heimuer	324:342	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	5	2	theme	unsaturated	720:730	arg1	acids					738:742	unsaturated fatty acids	720:742	60% unsaturated fatty acids	716:742	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	3	3	theme	dried	349:353	arg1	extract					355:361	The dried extract	345:361	The dried extract	345:361	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	6	4	theme	alkaline	821:828	arg1	conditions					838:847	alkaline (pH 10) conditions	821:847	alkaline (pH 10) conditions	821:847	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	5	5	dep	%	750:750	arg1	acids					768:772	essential amino acids	752:772	35% essential amino acids	748:772	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	2	6	theme	potential	278:286	arg1	use					288:290	potential use	278:290	potential use	278:290	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	6	7	theme	extract	879:885	arg1	thickness					854:862	the thickness	850:862	the thickness of the 5 mg/mL extract	850:885	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	2	8	from	heimuer	336:342	arg1	extract					311:317	the gel-forming extract	295:317	the gel-forming extract from Auricularia heimuer	295:342	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	2	8	from	heimuer	336:342	arg1	properties					263:272	properties	263:272	properties	263:272	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	2	8	from	heimuer	336:342	arg1	use					288:290	potential use	278:290	potential use	278:290	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	2	8	from	heimuer	336:342	arg1	composition					250:260	composition	250:260	composition	250:260	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	3	9	theme	xylose	524:529	arg1	acid					497:500	glucuronic acid	486:500	glucuronic acid	486:500	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	arabinose					564:572	arabinose	564:572	arabinose	564:572	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	galactose					532:540	galactose	532:540	galactose	532:540	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	glucosamine					543:553	glucosamine	543:553	glucosamine	543:553	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	residues					476:483	acetyl residues	469:483	acetyl residues	469:483	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	rhamnose					578:585	rhamnose	578:585	rhamnose	578:585	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	amount					514:519	a small amount	506:519	a small amount of xylose	506:529	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	mannose					448:454	mannose	448:454	mannose	448:454	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	xylose					524:529	xylose	524:529	xylose	524:529	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	fucose					556:561	fucose	556:561	fucose	556:561	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	9	theme	xylose	524:529	arg1	glucose					460:466	glucose	460:466	glucose	460:466	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	10	theme	acetyl	469:474	arg1	residues					476:483	acetyl residues	469:483	acetyl residues	469:483	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	10	theme	acetyl	469:474	arg1	xylose					524:529	xylose	524:529	xylose	524:529	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	5	11	theme	amino	703:707	arg1	acids					709:713	the fatty and amino acids	689:713	acids	709:713	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	7	12	theme	molecular	1187:1195	arg1	weight					1197:1202	the high molecular weight	1178:1202	the high molecular weight sodium hyaluronate	1178:1221	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	3	13	theme	small	508:512	arg1	xylose					524:529	xylose	524:529	xylose	524:529	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	13	theme	small	508:512	arg1	amount					514:519	a small amount	506:519	a small amount of xylose	506:529	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	7	14	theme	weight	1197:1202	arg1	moisturizer					1237:1247	a well-known moisturizer	1224:1247	a well-known moisturizer	1224:1247	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	7	14	theme	weight	1197:1202	arg1	hyaluronate					1211:1221	the high molecular weight sodium hyaluronate	1178:1221	the high molecular weight sodium hyaluronate	1178:1221	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	6	15	theme	mg/mL	873:877	arg1	extract					879:885	the 5 mg/mL extract	867:885	the 5 mg/mL extract	867:885	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	2	16	theme	extract	311:317	arg1	properties					263:272	properties	263:272	properties	263:272	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	2	16	theme	extract	311:317	arg1	use					288:290	potential use	278:290	potential use	278:290	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	2	16	theme	extract	311:317	arg1	composition					250:260	composition	250:260	composition	250:260	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	3	17	theme	%	375:375	arg1	homo-					385:389	50% soluble homo-	373:389	50% soluble homo-	373:389	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	0	18	from	Auricularia	32:42	arg1	Hydrocolloids					0:12	Hydrocolloids	0:12	Hydrocolloids from the Mushroom Auricularia	0:42	Hydrocolloids from the Mushroom Auricularia heimuer: Composition and Properties.
37367617	7	19	theme	storage	1104:1110	arg1	stability					1112:1120	good thermal and storage stability	1087:1120	stability	1112:1120	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	3	20	contain	contained	363:371	arg2	heteropolysaccharides					395:415	heteropolysaccharides	395:415	heteropolysaccharides	395:415	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	20	contain	contained	363:371	arg2	homo-					385:389	50% soluble homo-	373:389	50% soluble homo-	373:389	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	3	20	contain	contained	363:371	arg1	extract					355:361	The dried extract	345:361	The dried extract	345:361	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	2	21	theme	gel-forming	299:309	arg1	extract					311:317	the gel-forming extract	295:317	the gel-forming extract from Auricularia heimuer	295:342	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	7	22	theme	retention	1145:1153	arg1	capacity					1155:1162	a moisture retention capacity	1134:1162	a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer	1134:1247	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	6	23	theme	room	940:943	arg1	temperature					945:955	room temperature	940:955	room temperature	940:955	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	3	24	theme	soluble	377:383	arg1	homo-					385:389	50% soluble homo-	373:389	50% soluble homo-	373:389	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	6	25	theme	5	871:871	arg1	mg/mL					873:877	mg/mL	873:877	mg/mL	873:877	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	6	26	from	°C	934:935	arg1	range					919:923	a temperature range	905:923	a temperature range from -24 °C to room temperature	905:955	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	7	27	theme	sodium	1204:1209	arg1	moisturizer					1237:1247	a well-known moisturizer	1224:1247	a well-known moisturizer	1224:1247	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	7	27	theme	sodium	1204:1209	arg1	hyaluronate					1211:1221	the high molecular weight sodium hyaluronate	1178:1221	the high molecular weight sodium hyaluronate	1178:1221	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	0	28	theme	Mushroom	23:30	arg1	Auricularia					32:42	the Mushroom Auricularia	19:42	the Mushroom Auricularia	19:42	Hydrocolloids from the Mushroom Auricularia heimuer: Composition and Properties.
37367617	5	29	theme	essential	752:760	arg1	acids					768:772	essential amino acids	752:772	35% essential amino acids	748:772	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	5	30	dep	%	718:718	arg1	acids					738:742	unsaturated fatty acids	720:742	60% unsaturated fatty acids	716:742	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	1	31	theme	shell-shaped	93:104	arg1	bodies					115:120	shell-shaped fruiting bodies	93:120	shell-shaped fruiting bodies of the genus Auricularia	93:145	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	6	32	from	temperature	1026:1036	arg1	storage					1006:1012	storage	1006:1012	storage at elevated temperature	1006:1036	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	1	33	theme	traditional	178:188	arg1	remedies					200:207	traditional medicinal remedies	178:207	traditional medicinal remedies	178:207	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	3	34	theme	50	373:374	arg1	%					375:375	%	375:375	%	375:375	The dried extract contained 50% soluble homo- and heteropolysaccharides, which were mainly composed of mannose and glucose, acetyl residues, glucuronic acid and a small amount of xylose, galactose, glucosamine, fucose, arabinose and rhamnose.
37367617	4	35	dep	potassium	652:660	arg1	%					650:650	%	650:650	%	650:650	The minerals observed in the extract included approximately 70% potassium followed by calcium.
37367617	8	36	theme	potential	1354:1362	arg1	application					1342:1352	great application potential	1336:1362	great application potential	1336:1362	Hydrocolloids that can be sustainably produced from Auricularia fruiting bodies offer great application potential in the food and cosmetic industries.
37367617	1	37	theme	fruiting	106:113	arg1	bodies					115:120	shell-shaped fruiting bodies	93:120	shell-shaped fruiting bodies of the genus Auricularia	93:145	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	2	38	from	composition	250:260	arg1	heimuer					336:342	Auricularia heimuer	324:342	Auricularia heimuer	324:342	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	1	39	theme	medicinal	190:198	arg1	remedies					200:207	traditional medicinal remedies	178:207	traditional medicinal remedies	178:207	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	5	40	theme	fatty	732:736	arg1	acids					738:742	unsaturated fatty acids	720:742	60% unsaturated fatty acids	716:742	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	7	41	theme	studied	1058:1064	arg1	extract					1066:1072	the studied extract	1054:1072	the studied extract	1054:1072	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	7	42	theme	neutral	1042:1048	arg1	pH					1050:1051	neutral pH	1042:1051	neutral pH	1042:1051	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	5	43	theme	amino	762:766	arg1	acids					768:772	essential amino acids	752:772	35% essential amino acids	748:772	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	5	44	theme	fatty	693:697	arg1	acids					709:713	the fatty and amino acids	689:713	acids	709:713	Among the fatty and amino acids, 60% unsaturated fatty acids and 35% essential amino acids could be calculated.
37367617	8	45	theme	Auricularia	1302:1312	arg1	bodies					1323:1328	Auricularia fruiting bodies	1302:1328	Auricularia fruiting bodies	1302:1328	Hydrocolloids that can be sustainably produced from Auricularia fruiting bodies offer great application potential in the food and cosmetic industries.
37367617	6	46	theme	elevated	1017:1024	arg1	temperature					1026:1036	elevated temperature	1017:1036	elevated temperature	1017:1036	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	8	47	theme	food	1371:1374	arg1	industries					1389:1398	the food and cosmetic industries	1367:1398	the food and cosmetic industries	1367:1398	Hydrocolloids that can be sustainably produced from Auricularia fruiting bodies offer great application potential in the food and cosmetic industries.
37367617	7	48	theme	comparable	1164:1173	arg1	capacity					1155:1162	a moisture retention capacity	1134:1162	a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer	1134:1247	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	2	49	from	properties	263:272	arg1	heimuer					336:342	Auricularia heimuer	324:342	Auricularia heimuer	324:342	This study was primarily focused on the composition, properties and potential use of the gel-forming extract from Auricularia heimuer.
37367617	4	50	located	observed	601:608	arg2	minerals					592:599	The minerals	588:599	The minerals observed in the extract	588:623	The minerals observed in the extract included approximately 70% potassium followed by calcium.
37367617	4	50	located	observed	601:608	arg1	extract					617:623	the extract	613:623	the extract	613:623	The minerals observed in the extract included approximately 70% potassium followed by calcium.
37367617	7	51	theme	moisture	1136:1143	arg1	capacity					1155:1162	a moisture retention capacity	1134:1162	a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer	1134:1247	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	6	52	theme	temperature	907:917	arg1	range					919:923	a temperature range	905:923	a temperature range from -24 °C to room temperature	905:955	At both acidic (pH 4) and alkaline (pH 10) conditions, the thickness of the 5 mg/mL extract did not change in a temperature range from -24 °C to room temperature, but decreased statistically significantly after storage at elevated temperature.
37367617	4	53	dep	%	650:650	arg1	70					648:649	70	648:649	70	648:649	The minerals observed in the extract included approximately 70% potassium followed by calcium.
37367617	1	54	used	used	158:161	arg2	ear-					85:88	ear-	85:88	ear-	85:88	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	1	54	used	used	158:161	arg2	food					166:169	food	166:169	food	166:169	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	1	55	theme	genus	129:133	arg1	Auricularia					135:145	the genus Auricularia	125:145	the genus Auricularia	125:145	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	8	56	theme	fruiting	1314:1321	arg1	bodies					1323:1328	Auricularia fruiting bodies	1302:1328	Auricularia fruiting bodies	1302:1328	Hydrocolloids that can be sustainably produced from Auricularia fruiting bodies offer great application potential in the food and cosmetic industries.
37367617	8	57	theme	cosmetic	1380:1387	arg1	industries					1389:1398	the food and cosmetic industries	1367:1398	the food and cosmetic industries	1367:1398	Hydrocolloids that can be sustainably produced from Auricularia fruiting bodies offer great application potential in the food and cosmetic industries.
37367617	8	58	theme	great	1336:1340	arg1	application					1342:1352	great application potential	1336:1362	great application potential	1336:1362	Hydrocolloids that can be sustainably produced from Auricularia fruiting bodies offer great application potential in the food and cosmetic industries.
37367617	7	59	theme	high	1182:1185	arg1	weight					1197:1202	the high molecular weight	1178:1202	the high molecular weight sodium hyaluronate	1178:1221	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	1	60	theme	Auricularia	135:145	arg1	bodies					115:120	shell-shaped fruiting bodies	93:120	shell-shaped fruiting bodies of the genus Auricularia	93:145	The ear- to shell-shaped fruiting bodies of the genus Auricularia are widely used as food and in traditional medicinal remedies.
37367617	7	61	theme	well-known	1226:1235	arg1	moisturizer					1237:1247	a well-known moisturizer	1224:1247	a well-known moisturizer	1224:1247	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
37367617	7	61	theme	well-known	1226:1235	arg1	hyaluronate					1211:1221	the high molecular weight sodium hyaluronate	1178:1221	the high molecular weight sodium hyaluronate	1178:1221	At neutral pH, the studied extract demonstrated good thermal and storage stability, as well as a moisture retention capacity comparable to the high molecular weight sodium hyaluronate, a well-known moisturizer.
36739317	6	0	theme	greater	1049:1055	arg1	depths					1042:1047	depths	1042:1047	depths greater than 5500 m. Selfish uptake	1042:1083	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	4	1	theme	selfish	550:556	arg1	bacteria					558:565	selfish bacteria	550:565	selfish bacteria	550:565	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	0	2	theme	ocean	63:67	arg1	column					49:54	the water column	39:54	the water column of the ocean	39:67	Selfish bacteria are active throughout the water column of the ocean.
36739317	8	3	theme	polysaccharides	1487:1501	arg1	supply					1457:1462	the supply	1453:1462	the supply of structurally-intact polysaccharides	1453:1501	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	8	3	theme	polysaccharides	1487:1501	arg1	return					1522:1527	the return	1518:1527	the return on enzymatic investment	1518:1551	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	8	3	theme	polysaccharides	1487:1501	arg1	sufficient					1562:1571	sufficient	1562:1571	sufficient	1562:1571	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	5	4	theme	Atlantic	749:756	arg1	Ocean					758:762	the western North Atlantic Ocean	731:762	the western North Atlantic Ocean	731:762	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	6	5	theme	selfish	959:965	arg1	bacteria					967:974	selfish bacteria	959:974	selfish bacteria	959:974	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	8	6	theme	structurally-intact	1467:1485	arg1	polysaccharides					1487:1501	structurally-intact polysaccharides	1467:1501	structurally-intact polysaccharides	1467:1501	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	1	7	from	energy	135:140	arg1	enzymes					159:165	extracellular enzymes	145:165	extracellular enzymes	145:165	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	7	8	theme	molecular	1381:1389	arg1	weight					1391:1396	low molecular weight	1377:1396	low molecular weight substrates	1377:1407	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	5	9	theme	community	905:913	arg1	composition					915:925	microbial community composition	895:925	microbial community composition	895:925	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	8	10	from	return	1522:1527	arg1	investment					1542:1551	enzymatic investment	1532:1551	enzymatic investment	1532:1551	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	1	11	from	nitrogen	121:128	arg1	enzymes					159:165	extracellular enzymes	145:165	extracellular enzymes	145:165	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	5	12	theme	microbial	895:903	arg1	composition					915:925	microbial community composition	895:925	microbial community composition	895:925	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	6	13	dep	strategy	1090:1097	arg1	appears					1104:1110	appears	1104:1110	appears to be geographically-and phylogenetically-widespread	1104:1163	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	6	14	theme	geographically-and	1118:1135	arg1	phylogenetically-widespread					1137:1163	geographically-and phylogenetically-widespread	1118:1163	geographically-and phylogenetically-widespread	1118:1163	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	7	15	theme	bacteria	1303:1310	arg1	activities					1283:1292	the activities	1279:1292	the activities of these bacteria	1279:1310	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	5	16	theme	physiochemical	812:825	arg1	conditions					827:836	physiochemical conditions	812:836	physiochemical conditions	812:836	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	2	17	from	return	318:323	arg1	investment					333:342	this investment	328:342	this investment	328:342	Since hydrolysis products produced outside of a cell may be lost to diffusion, the return on this investment is uncertain.
36739317	1	18	theme	extracellular	145:157	arg1	enzymes					159:165	extracellular enzymes	145:165	extracellular enzymes	145:165	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	7	19	theme	polysaccharides	1197:1211	arg1	uptake					1187:1192	uptake	1187:1192	uptake	1187:1192	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	7	19	theme	polysaccharides	1197:1211	arg1	processing					1172:1181	processing	1172:1181	processing	1172:1181	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	3	20	dep	change	375:380	arg1	hydrolyzing					428:438	hydrolyzing	428:438	hydrolyzing	428:438	Selfish bacteria change the odds in their favor by binding, partially hydrolyzing, and transporting polysaccharides into the periplasmic space without loss of hydrolysis products.
36739317	3	20	dep	change	375:380	arg1	transporting					445:456	transporting	445:456	transporting polysaccharides into the periplasmic space without loss of hydrolysis products	445:535	Selfish bacteria change the odds in their favor by binding, partially hydrolyzing, and transporting polysaccharides into the periplasmic space without loss of hydrolysis products.
36739317	1	21	theme	Heterotrophic	70:82	arg1	bacteria					84:91	Heterotrophic bacteria	70:91	Heterotrophic bacteria in the ocean	70:104	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	7	22	theme	substrate	1258:1266	arg1	structure					1268:1276	substrate structure	1258:1276	substrate structure	1258:1276	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	4	23	theme	abundant	633:640	arg1	matter					656:661	abundant fresh organic matter	633:661	abundant fresh organic matter	633:661	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	0	24	theme	Selfish	0:6	arg1	bacteria					8:15	Selfish bacteria	0:15	Selfish bacteria	0:15	Selfish bacteria are active throughout the water column of the ocean.
36739317	7	25	theme	substrates	1398:1407	arg1	uptake					1362:1367	uptake	1362:1367	uptake only of low molecular weight substrates	1362:1407	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	6	26	theme	ocean	1022:1026	arg1	column					1008:1013	the water column	998:1013	the water column of the ocean	998:1026	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	3	27	theme	hydrolysis	517:526	arg1	products					528:535	hydrolysis products	517:535	hydrolysis products	517:535	Selfish bacteria change the odds in their favor by binding, partially hydrolyzing, and transporting polysaccharides into the periplasmic space without loss of hydrolysis products.
36739317	4	28	from	ocean	598:602	arg1	common					578:583	common	578:583	common	578:583	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	4	29	theme	upper	592:596	arg1	ocean					598:602	the upper ocean	588:602	the upper ocean	588:602	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	6	30	theme	Selfish	1070:1076	arg1	uptake					1078:1083	5500 m. Selfish uptake	1062:1083	5500 m. Selfish uptake	1062:1083	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	3	31	theme	products	528:535	arg1	loss					509:512	loss	509:512	loss of hydrolysis products	509:535	Selfish bacteria change the odds in their favor by binding, partially hydrolyzing, and transporting polysaccharides into the periplasmic space without loss of hydrolysis products.
36739317	7	32	theme	weight	1391:1396	arg1	substrates					1398:1407	low molecular weight substrates	1377:1407	low molecular weight substrates	1377:1407	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	1	33	theme	large	180:184	arg1	substrates					186:195	large substrates	180:195	large substrates	180:195	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	5	34	theme	western	735:741	arg1	Ocean					758:762	the western North Atlantic Ocean	731:762	the western North Atlantic Ocean	731:762	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	8	35	theme	enzymatic	1532:1540	arg1	investment					1542:1551	enzymatic investment	1532:1551	enzymatic investment	1532:1551	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	5	36	theme	North	743:747	arg1	Ocean					758:762	the western North Atlantic Ocean	731:762	the western North Atlantic Ocean	731:762	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	7	37	theme	low	1377:1379	arg1	weight					1391:1396	low molecular weight	1377:1396	low molecular weight substrates	1377:1407	Since processing and uptake of polysaccharides require enzymes that are highly sensitive to substrate structure, the activities of these bacteria might not be reflected by measurements relying on uptake only of low molecular weight substrates.
36739317	6	38	theme	water	1002:1006	arg1	column					1008:1013	the water column	998:1013	the water column of the ocean	998:1026	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	3	39	theme	Selfish	358:364	arg1	bacteria					366:373	Selfish bacteria	358:373	Selfish bacteria	358:373	Selfish bacteria change the odds in their favor by binding, partially hydrolyzing, and transporting polysaccharides into the periplasmic space without loss of hydrolysis products.
36739317	8	40	from	bottom	1432:1437	arg1	return					1522:1527	the return	1518:1527	the return on enzymatic investment	1518:1551	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	8	40	from	bottom	1432:1437	arg1	supply					1457:1462	the supply	1453:1462	the supply of structurally-intact polysaccharides	1453:1501	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	8	40	from	bottom	1432:1437	arg1	sufficient					1562:1571	sufficient	1562:1571	sufficient	1562:1571	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	6	41	theme	5500 m.	1062:1068	arg1	uptake					1078:1083	5500 m. Selfish uptake	1062:1083	5500 m. Selfish uptake	1062:1083	To our surprise, we found that selfish bacteria are common throughout the water column of the ocean, including at depths greater than 5500 m. Selfish uptake as a strategy thus appears to be geographically-and phylogenetically-widespread.
36739317	4	42	theme	complex	674:680	arg1	polysaccharides					682:696	complex polysaccharides	674:696	complex polysaccharides	674:696	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	0	43	theme	water	43:47	arg1	column					49:54	the water column	39:54	the water column of the ocean	39:67	Selfish bacteria are active throughout the water column of the ocean.
36739317	8	44	theme	ocean	1446:1450	arg1	bottom					1432:1437	the bottom	1428:1437	the bottom of the ocean	1428:1450	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	1	45	from	carbon	113:118	arg1	enzymes					159:165	extracellular enzymes	145:165	extracellular enzymes	145:165	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	2	46	theme	hydrolysis	241:250	arg1	products					252:259	hydrolysis products	241:259	hydrolysis products produced outside of a cell	241:286	Since hydrolysis products produced outside of a cell may be lost to diffusion, the return on this investment is uncertain.
36739317	5	47	from	stations	790:797	arg1	depths					772:777	four depths	767:777	four depths from three stations differing in physiochemical conditions	767:836	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	5	48	dep	stations	845:852	arg1	these					839:843	these	839:843	these	839:843	We, therefore, sampled water in the western North Atlantic Ocean at four depths from three stations differing in physiochemical conditions; these stations and depths also differed considerably in microbial community composition.
36739317	1	49	theme	smaller	200:206	arg1	sizes					208:212	smaller sizes	200:212	smaller sizes suitable for uptake	200:232	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	3	50	from	odds	386:389	arg1	favor					400:404	favor	400:404	favor	400:404	Selfish bacteria change the odds in their favor by binding, partially hydrolyzing, and transporting polysaccharides into the periplasmic space without loss of hydrolysis products.
36739317	4	51	theme	fresh	642:646	arg1	matter					656:661	abundant fresh organic matter	633:661	abundant fresh organic matter	633:661	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	4	52	theme	organic	648:654	arg1	matter					656:661	abundant fresh organic matter	633:661	abundant fresh organic matter	633:661	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	1	53	from	bacteria	84:91	arg1	ocean					100:104	the ocean	96:104	the ocean	96:104	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	1	54	theme	suitable	214:221	arg1	sizes					208:212	smaller sizes	200:212	smaller sizes suitable for uptake	200:232	Heterotrophic bacteria in the ocean invest carbon, nitrogen, and energy in extracellular enzymes to hydrolyze large substrates to smaller sizes suitable for uptake.
36739317	4	55	from	common	578:583	arg1	ocean					598:602	the upper ocean	588:602	the upper ocean	588:602	We expected selfish bacteria to be most common in the upper ocean, where phytoplankton produce abundant fresh organic matter, including complex polysaccharides.
36739317	8	56	from	supply	1457:1462	arg1	investment					1542:1551	enzymatic investment	1532:1551	enzymatic investment	1532:1551	Moreover, even at the bottom of the ocean, the supply of structurally-intact polysaccharides, and therefore the return on enzymatic investment, must be sufficient to maintain these organisms.
36739317	3	57	theme	periplasmic	483:493	arg1	space					495:499	the periplasmic space	479:499	the periplasmic space without loss of hydrolysis products	479:535	Selfish bacteria change the odds in their favor by binding, partially hydrolyzing, and transporting polysaccharides into the periplasmic space without loss of hydrolysis products.
35625898	4	0	from	chemosensitivity	906:921	arg1	cells					970:974	both cells	965:974	both cells	965:974	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	4	1	from	defects	1041:1047	arg1	sublines					1067:1074	chemoresistant sublines	1052:1074	chemoresistant sublines	1052:1074	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	6	2	theme	upregulated	1298:1308	arg1	genes					1310:1314	97 upregulated genes	1295:1314	97 upregulated genes	1295:1314	Transcriptome analysis of these cells revealed 97 upregulated genes, which were enriched in multiple oncogenic pathways.
35625898	3	3	with	models	700:705	arg1	resistance					721:730	acquired resistance	712:730	acquired resistance to gemcitabine and paclitaxel	712:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	1	4	theme	bladder	318:324	arg1	carcinoma					301:309	urothelial carcinoma	290:309	urothelial carcinoma of the bladder (UCB)	290:330	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	4	5	theme	corresponding	930:942	arg1	chemotherapeutics					944:960	the corresponding chemotherapeutics	926:960	the corresponding chemotherapeutics	926:960	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	7	6	theme	growth	1499:1504	arg1	defects					1506:1512	cell growth defects	1494:1512	cell growth defects	1494:1512	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	2	7	theme	diverse	601:607	arg1	phenotypes					621:630	diverse cancer cell phenotypes	601:630	diverse cancer cell phenotypes	601:630	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	0	8	theme	Aggressive	144:153	arg1	Traits					161:166	Aggressive Tumor Traits	144:166	Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder	144:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	5	9	from	effects	1118:1124	arg1	migration/invasion					1156:1173	cell migration/invasion	1151:1173	cell migration/invasion in vitro	1151:1182	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	5	9	from	effects	1118:1124	arg1	growth					1204:1209	xenograft tumor growth	1188:1209	xenograft tumor growth	1188:1209	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	1	10	theme	acquired	238:245	arg1	chemoresistance					247:261	acquired chemoresistance	238:261	acquired chemoresistance	238:261	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	6	11	theme	oncogenic	1349:1357	arg1	pathways					1359:1366	multiple oncogenic pathways	1340:1366	multiple oncogenic pathways	1340:1366	Transcriptome analysis of these cells revealed 97 upregulated genes, which were enriched in multiple oncogenic pathways.
35625898	5	12	theme	xenograft	1188:1196	arg1	growth					1204:1209	xenograft tumor growth	1188:1209	xenograft tumor growth	1188:1209	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	0	13	theme	Chemoresistant	171:184	arg1	Carcinoma					197:205	Chemoresistant Urothelial Carcinoma	171:205	Chemoresistant Urothelial Carcinoma of the Bladder	171:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	2	14	theme	cell	616:619	arg1	phenotypes					621:630	diverse cancer cell phenotypes	601:630	diverse cancer cell phenotypes	601:630	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	8	15	theme	dysregulated	1560:1571	arg1	activity					1577:1584	dysregulated OGT activity	1560:1584	dysregulated OGT activity	1560:1584	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	7	16	theme	PRKCG	1456:1460	arg1	knockdown					1467:1475	the knockdown	1463:1475	the knockdown	1463:1475	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	1	17	from	cycles	280:285	arg1	carcinoma					301:309	urothelial carcinoma	290:309	urothelial carcinoma of the bladder (UCB)	290:330	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	8	18	theme	activity	1577:1584	arg1	role					1552:1555	the vital role	1542:1555	the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB	1542:1685	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	0	19	theme	Promising	71:79	arg1	Strategy					93:100	a Promising Therapeutic Strategy	69:100	a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder	69:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	0	19	theme	Promising	71:79	arg1	Inhibition					9:18	Inhibition	9:18	Inhibition of O-Linked β-N-Acetylglucosamine Transferase	9:64	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	5	20	theme	chemoresistant	1222:1235	arg1	cells					1241:1245	chemoresistant UCB cells	1222:1245	chemoresistant UCB cells	1222:1245	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	3	21	theme	expression	660:669	arg1	levels					671:676	OGT expression levels	656:676	OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel	656:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	8	22	theme	hyper-O-GlcNAcylation	1590:1610	arg1	role					1552:1555	the vital role	1542:1555	the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB	1542:1685	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	4	23	theme	combination	981:991	arg1	treatment					993:1001	combination treatment	981:1001	combination treatment with OGT-KD	981:1013	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	4	24	theme	growth	1034:1039	arg1	defects					1041:1047	more severe growth defects	1022:1047	more severe growth defects in chemoresistant sublines	1022:1074	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	2	25	theme	key	553:555	arg1	signaling					557:565	key signaling and metabolic pathways	553:588	signaling	557:565	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	2	26	theme	Elevated	425:432	arg1	flux					442:445	Elevated glucose flux	425:445	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	425:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	1	27	theme	cancer	390:395	arg1	progression					397:407	cancer progression	390:407	cancer progression	390:407	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	7	28	theme	PDGFRB	1444:1449	arg1	knockdown					1467:1475	the knockdown	1463:1475	the knockdown	1463:1475	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	2	29	theme	O-linked	484:491	arg1	OGT					539:541	OGT	539:541	OGT	539:541	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	2	29	theme	O-linked	484:491	arg1	transferase					526:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase	484:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	484:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	8	30	theme	tumor	1648:1652	arg1	aggression					1654:1663	tumor aggression	1648:1663	tumor aggression	1648:1663	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	4	31	with	treatment	993:1001	arg1	OGT-KD					1008:1013	OGT-KD	1008:1013	OGT-KD	1008:1013	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	7	32	theme	S1PR3	1437:1441	arg1	knockdown					1467:1475	the knockdown	1463:1475	the knockdown	1463:1475	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	7	33	theme	VCAN	1431:1434	arg1	knockdown					1467:1475	the knockdown	1463:1475	the knockdown	1463:1475	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	3	34	theme	cell	695:698	arg1	models					700:705	two human UCB cell models	681:705	two human UCB cell models with acquired resistance to gemcitabine and paclitaxel	681:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	0	35	theme	β-N-Acetylglucosamine	32:52	arg1	Transferase					54:64	O-Linked β-N-Acetylglucosamine Transferase	23:64	O-Linked β-N-Acetylglucosamine Transferase	23:64	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	7	36	theme	substrate	1417:1425	arg1	choice					1379:1384	Our final choice	1369:1384	Our final choice of suspected OGT glycosylation substrate	1369:1425	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	3	37	theme	human	685:689	arg1	models					700:705	two human UCB cell models	681:705	two human UCB cell models with acquired resistance to gemcitabine and paclitaxel	681:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	1	38	theme	death	354:358	arg1	Acquisition					223:233	Acquisition	223:233	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB)	223:330	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	1	38	theme	death	354:358	arg1	cause					345:349	the major cause	335:349	the major cause of death	335:358	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	5	39	theme	monotherapy	1136:1146	arg1	effects					1118:1124	the suppressive effects	1102:1124	the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth	1102:1209	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	7	40	theme	OGT	1399:1401	arg1	substrate					1417:1425	suspected OGT glycosylation substrate	1389:1425	suspected OGT glycosylation substrate	1389:1425	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	4	41	theme	OGT	866:868	arg1	knockdown					870:878	OGT knockdown	866:878	OGT knockdown (KD)	866:883	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	4	41	theme	OGT	866:868	arg1	KD					881:882	KD	881:882	KD	881:882	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	1	42	theme	treatment	270:278	arg1	cycles					280:285	treatment cycles	270:285	treatment cycles in urothelial carcinoma of the bladder (UCB)	270:330	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	0	43	from	Traits	161:166	arg1	Carcinoma					197:205	Chemoresistant Urothelial Carcinoma	171:205	Chemoresistant Urothelial Carcinoma of the Bladder	171:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	5	44	theme	suppressive	1106:1116	arg1	effects					1118:1124	the suppressive effects	1102:1124	the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth	1102:1209	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	7	45	theme	final	1373:1377	arg1	choice					1379:1384	Our final choice	1369:1384	Our final choice of suspected OGT glycosylation substrate	1369:1425	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	0	46	theme	Bladder	214:220	arg1	Carcinoma					197:205	Chemoresistant Urothelial Carcinoma	171:205	Chemoresistant Urothelial Carcinoma of the Bladder	171:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	6	47	theme	cells	1280:1284	arg1	analysis					1262:1269	Transcriptome analysis	1248:1269	Transcriptome analysis of these cells	1248:1284	Transcriptome analysis of these cells revealed 97 upregulated genes, which were enriched in multiple oncogenic pathways.
35625898	3	48	theme	parental	816:823	arg1	cells					825:829	parental cells	816:829	parental cells	816:829	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	0	49	theme	Tumor	155:159	arg1	Traits					161:166	Aggressive Tumor Traits	144:166	Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder	144:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	8	50	theme	vital	1546:1550	arg1	role					1552:1555	the vital role	1542:1555	the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB	1542:1685	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	6	51	theme	Transcriptome	1248:1260	arg1	analysis					1262:1269	Transcriptome analysis	1248:1269	Transcriptome analysis of these cells	1248:1284	Transcriptome analysis of these cells revealed 97 upregulated genes, which were enriched in multiple oncogenic pathways.
35625898	1	52	theme	metastasis	413:422	arg1	risk					382:385	the risk	378:385	the risk of cancer progression and metastasis	378:422	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	1	53	theme	chemoresistance	247:261	arg1	Acquisition					223:233	Acquisition	223:233	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB)	223:330	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	1	53	theme	chemoresistance	247:261	arg1	cause					345:349	the major cause	335:349	the major cause of death	335:358	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	2	54	theme	metabolic	571:579	arg1	pathways					581:588	key signaling and metabolic pathways	553:588	pathways	581:588	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	6	55	theme	multiple	1340:1347	arg1	pathways					1359:1366	multiple oncogenic pathways	1340:1366	multiple oncogenic pathways	1340:1366	Transcriptome analysis of these cells revealed 97 upregulated genes, which were enriched in multiple oncogenic pathways.
35625898	0	56	theme	Urothelial	186:195	arg1	Carcinoma					197:205	Chemoresistant Urothelial Carcinoma	171:205	Chemoresistant Urothelial Carcinoma of the Bladder	171:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	5	57	theme	tumor	1198:1202	arg1	growth					1204:1209	xenograft tumor growth	1188:1209	xenograft tumor growth	1188:1209	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	8	58	theme	OGT	1573:1575	arg1	activity					1577:1584	dysregulated OGT activity	1560:1584	dysregulated OGT activity	1560:1584	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	5	59	theme	cell	1151:1154	arg1	migration/invasion					1156:1173	cell migration/invasion	1151:1173	cell migration/invasion in vitro	1151:1182	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	2	60	theme	cancer	609:614	arg1	phenotypes					621:630	diverse cancer cell phenotypes	601:630	diverse cancer cell phenotypes	601:630	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	0	61	theme	Therapeutic	81:91	arg1	Strategy					93:100	a Promising Therapeutic Strategy	69:100	a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder	69:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	0	61	theme	Therapeutic	81:91	arg1	Inhibition					9:18	Inhibition	9:18	Inhibition of O-Linked β-N-Acetylglucosamine Transferase	9:64	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	3	62	theme	OGT	656:658	arg1	levels					671:676	OGT expression levels	656:676	OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel	656:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	4	63	theme	chemoresistant	1052:1065	arg1	sublines					1067:1074	chemoresistant sublines	1052:1074	chemoresistant sublines	1052:1074	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	5	64	theme	UCB	1237:1239	arg1	cells					1241:1245	chemoresistant UCB cells	1222:1245	chemoresistant UCB cells	1222:1245	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	2	65	theme	transferase	526:536	arg1	upregulation					468:479	the abnormal upregulation	455:479	the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	455:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	8	66	theme	treatment	1626:1634	arg1	failure					1636:1642	treatment failure	1626:1642	treatment failure	1626:1642	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	7	67	theme	cell	1494:1497	arg1	defects					1506:1512	cell growth defects	1494:1512	cell growth defects	1494:1512	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	4	68	theme	severe	1027:1032	arg1	defects					1041:1047	more severe growth defects	1022:1047	more severe growth defects in chemoresistant sublines	1022:1074	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	2	69	link	O-linked	484:491	arg1	OGT					539:541	OGT	539:541	OGT	539:541	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	2	69	link	O-linked	484:491	arg1	transferase					526:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase	484:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	484:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	4	70	theme	Reducing	832:839	arg1	hyper-O-GlcNAcylation					841:861	Reducing hyper-O-GlcNAcylation	832:861	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD)	832:883	Reducing hyper-O-GlcNAcylation by OGT knockdown (KD) markedly facilitated chemosensitivity to the corresponding chemotherapeutics in both cells, and combination treatment with OGT-KD showed more severe growth defects in chemoresistant sublines.
35625898	3	71	from	levels	671:676	arg1	models					700:705	two human UCB cell models	681:705	two human UCB cell models with acquired resistance to gemcitabine and paclitaxel	681:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	1	72	theme	progression	397:407	arg1	risk					382:385	the risk	378:385	the risk of cancer progression and metastasis	378:422	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	8	73	theme	chemoresistant	1668:1681	arg1	UCB					1683:1685	chemoresistant UCB	1668:1685	chemoresistant UCB	1668:1685	These findings demonstrate the vital role of dysregulated OGT activity and hyper-O-GlcNAcylation in modulating treatment failure and tumor aggression in chemoresistant UCB.
35625898	0	74	theme	O-Linked	23:30	arg1	Transferase					54:64	O-Linked β-N-Acetylglucosamine Transferase	23:64	O-Linked β-N-Acetylglucosamine Transferase	23:64	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	2	75	theme	abnormal	459:466	arg1	upregulation					468:479	the abnormal upregulation	455:479	the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	455:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	3	76	theme	UCB	691:693	arg1	models					700:705	two human UCB cell models	681:705	two human UCB cell models with acquired resistance to gemcitabine and paclitaxel	681:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	0	77	theme	Transferase	54:64	arg1	Strategy					93:100	a Promising Therapeutic Strategy	69:100	a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder	69:220	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	0	77	theme	Transferase	54:64	arg1	Inhibition					9:18	Inhibition	9:18	Inhibition of O-Linked β-N-Acetylglucosamine Transferase	9:64	Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder.
35625898	7	78	theme	glycosylation	1403:1415	arg1	substrate					1417:1425	suspected OGT glycosylation substrate	1389:1425	suspected OGT glycosylation substrate	1389:1425	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	2	79	theme	O-GlcNAc	516:523	arg1	OGT					539:541	OGT	539:541	OGT	539:541	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	2	79	theme	O-GlcNAc	516:523	arg1	transferase					526:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase	484:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	484:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	2	80	theme	glucose	434:440	arg1	flux					442:445	Elevated glucose flux	425:445	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	425:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	1	81	theme	major	339:343	arg1	Acquisition					223:233	Acquisition	223:233	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB)	223:330	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	1	81	theme	major	339:343	arg1	cause					345:349	the major cause	335:349	the major cause of death	335:358	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
35625898	5	82	theme	OGT-KD	1129:1134	arg1	monotherapy					1136:1146	OGT-KD monotherapy	1129:1146	OGT-KD monotherapy	1129:1146	We subsequently verified the suppressive effects of OGT-KD monotherapy on cell migration/invasion in vitro and xenograft tumor growth in vivo in chemoresistant UCB cells.
35625898	7	83	theme	suspected	1389:1397	arg1	substrate					1417:1425	suspected OGT glycosylation substrate	1389:1425	suspected OGT glycosylation substrate	1389:1425	Our final choice of suspected OGT glycosylation substrate was VCAN, S1PR3, PDGFRB, and PRKCG, the knockdown of which induced cell growth defects.
35625898	2	84	theme	β-N-acetylglucosamine	493:513	arg1	OGT					539:541	OGT	539:541	OGT	539:541	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	2	84	theme	β-N-acetylglucosamine	493:513	arg1	transferase					526:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase	484:536	O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT)	484:542	Elevated glucose flux through the abnormal upregulation of O-linked β-N-acetylglucosamine (O-GlcNAc) transferase (OGT) controls key signaling and metabolic pathways regulating diverse cancer cell phenotypes.
35625898	3	85	theme	acquired	712:719	arg1	resistance					721:730	acquired resistance	712:730	acquired resistance to gemcitabine and paclitaxel	712:760	This study showed that OGT expression levels in two human UCB cell models with acquired resistance to gemcitabine and paclitaxel were significantly upregulated compared with those in parental cells.
35625898	1	86	theme	urothelial	290:299	arg1	carcinoma					301:309	urothelial carcinoma	290:309	urothelial carcinoma of the bladder (UCB)	290:330	Acquisition of acquired chemoresistance during treatment cycles in urothelial carcinoma of the bladder (UCB) is the major cause of death through enhancing the risk of cancer progression and metastasis.
36699583	7	0	theme	polysaccharides	1209:1223	arg1	proportions					1142:1152	the relative proportions	1129:1152	the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM	1129:1234	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	5	1	theme	spectrometry	647:658	arg1	techniques					691:700	pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques	613:700	pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques	613:700	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	4	2	theme	incubation	562:571	arg1	experiment					573:582	a FTCs incubation experiment	555:582	a FTCs incubation experiment	555:582	Here, a FTCs incubation experiment was conducted.
36699583	10	3	theme	relative	1529:1536	arg1	abundance					1538:1546	the relative abundance	1525:1546	the relative abundance of these taxa	1525:1560	Moreover, the relative abundance of these taxa was positively correlated with the increment of DOC.
36699583	5	4	theme	high-throughput	664:678	arg1	sequencing					680:689	high-throughput sequencing	664:689	high-throughput sequencing	664:689	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	2	5	theme	cycles	276:281	arg1	FTCs					284:287	increased freeze-thaw cycles (FTCs)	254:288	increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption	254:360	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	6	6	theme	organic	932:938	arg1	DOC					948:950	DOC	948:950	DOC	948:950	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	6	6	theme	organic	932:938	arg1	carbon					940:945	organic carbon	932:945	organic carbon (DOC) contents	932:960	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	14	7	theme	molecular	2232:2240	arg1	mechanisms					2242:2251	DOM molecular mechanisms	2228:2251	DOM molecular mechanisms	2228:2251	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	11	8	theme	Co-occurrence	1615:1627	arg1	networks					1629:1636	Co-occurrence networks	1615:1636	Co-occurrence networks	1615:1636	Co-occurrence networks confirmed DOM-bacterial interactions, implying that specific microorganisms degrade specific substrates.
36699583	7	9	theme	aromatic	1095:1102	arg1	compounds					1104:1112	aromatic compounds	1095:1112	aromatic compounds	1095:1112	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	6	10	theme	low-frequency	825:837	arg1	2FTCs					840:844	both low-frequency (2FTCs)	820:845	both low-frequency (2FTCs)	820:845	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	2	11	theme	increased	254:262	arg1	FTCs					284:287	increased freeze-thaw cycles (FTCs)	254:288	increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption	254:360	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	12	12	theme	aromatic	2008:2015	arg1	compounds					2017:2025	aromatic compounds	2008:2025	aromatic compounds in the topsoil	2008:2040	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	10	13	theme	taxa	1557:1560	arg1	abundance					1538:1546	the relative abundance	1525:1546	the relative abundance of these taxa	1525:1560	Moreover, the relative abundance of these taxa was positively correlated with the increment of DOC.
36699583	5	14	theme	gas	623:625	arg1	spectrometry					647:658	pyrolysis gas chromatography-mass spectrometry	613:658	pyrolysis gas chromatography-mass spectrometry	613:658	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	8	15	theme	subsuface	1244:1252	arg1	layer					1259:1263	the subsuface soil layer	1240:1263	the subsuface soil layer	1240:1263	In the subsuface soil layer, only the relative abundance of Me in the 6FTCs treatment increased significantly.
36699583	0	16	theme	freeze-thaw	126:136	arg1	cycles					138:143	freeze-thaw cycles	126:143	freeze-thaw cycles	126:143	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	13	17	theme	C	2169:2169	arg1	sequestration					2171:2183	C sequestration	2169:2183	C sequestration	2169:2183	Collectively, FTCs tended to activate DOM and enhance its biodegradability of DOM, potentially hampering DOC accumulation and C sequestration.
36699583	7	18	theme	alkanes	1157:1163	arg1	proportions					1142:1152	the relative proportions	1129:1152	the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM	1129:1234	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	6	19	dep	surface	969:975	arg1	layers					1018:1023	soil layers	1013:1023	soil layers	1013:1023	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	9	20	theme	relative	1454:1461	arg1	abundance					1463:1471	only the relative abundance	1445:1471	only the relative abundance of Gammaproteobacteria	1445:1494	The response of bacterial communities to FTCs was more sensitive than that of fungi, among which only the relative abundance of Gammaproteobacteria increased by FTCs.
36699583	8	21	theme	relative	1275:1282	arg1	abundance					1284:1292	only the relative abundance	1266:1292	only the relative abundance of Me in the 6FTCs treatment	1266:1321	In the subsuface soil layer, only the relative abundance of Me in the 6FTCs treatment increased significantly.
36699583	0	22	theme	matter	64:69	arg1	composition					31:41	the molecular composition	17:41	the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles	17:143	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	2	23	theme	Global	230:235	arg1	warming					237:243	Global warming	230:243	Global warming	230:243	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	0	24	theme	soil	75:78	arg1	community					90:98	soil microbial community	75:98	soil microbial community	75:98	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	1	25	theme	organic	161:167	arg1	DOM					177:179	DOM	177:179	DOM	177:179	Soil dissolved organic matter (DOM) plays a vital role in biogeochemical processes.
36699583	1	25	theme	organic	161:167	arg1	matter					169:174	organic matter	161:174	organic matter (DOM)	161:180	Soil dissolved organic matter (DOM) plays a vital role in biogeochemical processes.
36699583	0	26	theme	community	90:98	arg1	composition					31:41	the molecular composition	17:41	the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles	17:143	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	8	27	theme	6FTCs	1307:1311	arg1	treatment					1313:1321	the 6FTCs treatment	1303:1321	the 6FTCs treatment	1303:1321	In the subsuface soil layer, only the relative abundance of Me in the 6FTCs treatment increased significantly.
36699583	12	28	dep	correlated	1809:1818	arg1	alkanes					1855:1861	alkanes	1855:1861	alkanes	1855:1861	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	28	dep	correlated	1809:1818	arg1	whereas					1865:1871	whereas	1865:1871	whereas	1865:1871	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	28	dep	correlated	1809:1818	arg1	polysaccharides					1835:1849	polysaccharides	1835:1849	polysaccharides	1835:1849	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	7	29	theme	acid	1181:1184	arg1	Me					1201:1202	Me	1201:1202	Me	1201:1202	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	7	29	theme	acid	1181:1184	arg1	esters					1193:1198	fatty acid methyl esters	1175:1198	fatty acid methyl esters (Me)	1175:1203	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	0	30	from	composition	31:41	arg1	forest					112:117	a boreal forest	103:117	a boreal forest during freeze-thaw cycles	103:143	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	14	31	theme	increased	2323:2331	arg1	FTCs					2333:2336	increased FTCs	2323:2336	increased FTCs	2323:2336	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	14	32	theme	communities	2305:2315	arg1	states					2280:2285	the functional states	2265:2285	the functional states of soil bacterial communities	2265:2315	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	6	33	theme	increased	907:915	arg1	soil					917:920	significantly increased soil	893:920	freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers	866:1023	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	6	34	dep	cycles	878:883	arg1	soil					917:920	significantly increased soil	893:920	freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers	866:1023	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	14	35	theme	soil	2290:2293	arg1	communities					2305:2315	soil bacterial communities	2290:2315	soil bacterial communities	2290:2315	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	3	36	theme	DOM	425:427	arg1	molecules					429:437	DOM molecules	425:437	DOM molecules	425:437	However, the interactions between the chemical composition of DOM molecules and the microbial communities that drive C decomposition in the context of freeze-thaw are poorly understood.
36699583	12	37	theme	bacterial	1879:1887	arg1	Actinobacteria					1905:1918	Actinobacteria	1905:1918	Actinobacteria	1905:1918	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	37	theme	bacterial	1879:1887	arg1	Thermoleophilia					1946:1960	Thermoleophilia	1946:1960	Thermoleophilia	1946:1960	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	37	theme	bacterial	1879:1887	arg1	Alphaproteobacteria					1921:1939	Alphaproteobacteria	1921:1939	Alphaproteobacteria	1921:1939	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	37	theme	bacterial	1879:1887	arg1	classes					1889:1895	other bacterial classes	1873:1895	other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia	1873:1960	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	0	38	theme	dissolved	46:54	arg1	matter					64:69	dissolved organic matter	46:69	dissolved organic matter	46:69	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	11	39	theme	specific	1722:1729	arg1	substrates					1731:1740	specific substrates	1722:1740	specific substrates	1722:1740	Co-occurrence networks confirmed DOM-bacterial interactions, implying that specific microorganisms degrade specific substrates.
36699583	3	40	theme	microbial	447:455	arg1	communities					457:467	the microbial communities	443:467	the microbial communities that drive C decomposition in the context of freeze-thaw	443:524	However, the interactions between the chemical composition of DOM molecules and the microbial communities that drive C decomposition in the context of freeze-thaw are poorly understood.
36699583	6	41	theme	freeze-thaw	866:876	arg1	cycles					878:883	freeze-thaw cycles	866:883	freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers	866:1023	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	6	41	theme	freeze-thaw	866:876	arg1	6FTCs					886:890	6FTCs	886:890	6FTCs	886:890	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	7	42	theme	fatty	1175:1179	arg1	Me					1201:1202	Me	1201:1202	Me	1201:1202	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	7	42	theme	fatty	1175:1179	arg1	esters					1193:1198	fatty acid methyl esters	1175:1198	fatty acid methyl esters (Me)	1175:1203	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	14	43	theme	functional	2269:2278	arg1	states					2280:2285	the functional states	2265:2285	the functional states of soil bacterial communities	2265:2315	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	5	44	theme	chromatography-mass	627:645	arg1	spectrometry					647:658	pyrolysis gas chromatography-mass spectrometry	613:658	pyrolysis gas chromatography-mass spectrometry	613:658	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	3	45	theme	C	480:480	arg1	decomposition					482:494	C decomposition	480:494	C decomposition	480:494	However, the interactions between the chemical composition of DOM molecules and the microbial communities that drive C decomposition in the context of freeze-thaw are poorly understood.
36699583	9	46	theme	communities	1374:1384	arg1	response					1352:1359	The response	1348:1359	The response of bacterial communities to FTCs	1348:1392	The response of bacterial communities to FTCs was more sensitive than that of fungi, among which only the relative abundance of Gammaproteobacteria increased by FTCs.
36699583	9	46	theme	communities	1374:1384	arg1	sensitive					1403:1411	sensitive	1403:1411	sensitive	1403:1411	The response of bacterial communities to FTCs was more sensitive than that of fungi, among which only the relative abundance of Gammaproteobacteria increased by FTCs.
36699583	7	47	theme	relative	1073:1080	arg1	abundance					1082:1090	the relative abundance	1069:1090	the relative abundance of aromatic compounds	1069:1112	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	7	48	theme	compounds	1104:1112	arg1	abundance					1082:1090	the relative abundance	1069:1090	the relative abundance of aromatic compounds	1069:1112	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	3	49	theme	freeze-thaw	514:524	arg1	context					503:509	the context	499:509	the context of freeze-thaw	499:524	However, the interactions between the chemical composition of DOM molecules and the microbial communities that drive C decomposition in the context of freeze-thaw are poorly understood.
36699583	6	50	theme	carbon	940:945	arg1	contents					953:960	organic carbon (DOC) contents	932:960	organic carbon (DOC) contents	932:960	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	4	51	theme	FTCs	557:560	arg1	experiment					573:582	a FTCs incubation experiment	555:582	a FTCs incubation experiment	555:582	Here, a FTCs incubation experiment was conducted.
36699583	14	52	theme	mechanisms	2242:2251	arg1	potential					2215:2223	the potential	2211:2223	the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs	2211:2336	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	11	53	theme	DOM-bacterial	1648:1660	arg1	interactions					1662:1673	DOM-bacterial interactions	1648:1673	DOM-bacterial interactions	1648:1673	Co-occurrence networks confirmed DOM-bacterial interactions, implying that specific microorganisms degrade specific substrates.
36699583	2	54	theme	freeze-thaw	264:274	arg1	FTCs					284:287	increased freeze-thaw cycles (FTCs)	254:288	increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption	254:360	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	7	55	theme	relative	1133:1140	arg1	proportions					1142:1152	the relative proportions	1129:1152	the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM	1129:1234	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	14	56	theme	DOM	2228:2230	arg1	mechanisms					2242:2251	DOM molecular mechanisms	2228:2251	DOM molecular mechanisms	2228:2251	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	5	57	theme	pyrolysis	613:621	arg1	spectrometry					647:658	pyrolysis gas chromatography-mass spectrometry	613:658	pyrolysis gas chromatography-mass spectrometry	613:658	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	2	58	theme	boreal	293:298	arg1	soils					307:311	boreal forest soils	293:311	boreal forest soils	293:311	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	12	59	theme	class	1746:1750	arg1	level					1752:1756	class level	1746:1756	class level	1746:1756	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	5	60	theme	DOM	729:731	arg1	chemodiversity					733:746	DOM chemodiversity	729:746	DOM chemodiversity	729:746	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	1	61	theme	vital	190:194	arg1	role					196:199	a vital role	188:199	a vital role	188:199	Soil dissolved organic matter (DOM) plays a vital role in biogeochemical processes.
36699583	5	62	theme	microbial	752:760	arg1	structure					772:780	microbial community structure	752:780	microbial community structure	752:780	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	5	63	theme	sequencing	680:689	arg1	techniques					691:700	pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques	613:700	pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques	613:700	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	8	64	theme	soil	1254:1257	arg1	layer					1259:1263	the subsuface soil layer	1240:1263	the subsuface soil layer	1240:1263	In the subsuface soil layer, only the relative abundance of Me in the 6FTCs treatment increased significantly.
36699583	13	65	theme	DOC	2148:2150	arg1	accumulation					2152:2163	DOC accumulation	2148:2163	DOC accumulation	2148:2163	Collectively, FTCs tended to activate DOM and enhance its biodegradability of DOM, potentially hampering DOC accumulation and C sequestration.
36699583	6	66	theme	soil	1013:1016	arg1	layers					1018:1023	soil layers	1013:1023	soil layers	1013:1023	Results indicated that both low-frequency (2FTCs) and high-frequency freeze-thaw cycles (6FTCs) significantly increased soil dissolved organic carbon (DOC) contents in the surface (0-10 cm) and subsurface (50-60 cm) soil layers.
36699583	7	67	theme	esters	1193:1198	arg1	proportions					1142:1152	the relative proportions	1129:1152	the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM	1129:1234	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	0	68	theme	microbial	80:88	arg1	community					90:98	soil microbial community	75:98	soil microbial community	75:98	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	9	69	theme	Gammaproteobacteria	1476:1494	arg1	abundance					1463:1471	only the relative abundance	1445:1471	only the relative abundance of Gammaproteobacteria	1445:1494	The response of bacterial communities to FTCs was more sensitive than that of fungi, among which only the relative abundance of Gammaproteobacteria increased by FTCs.
36699583	2	70	from	FTCs	284:287	arg1	soils					307:311	boreal forest soils	293:311	boreal forest soils	293:311	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	7	71	from	proportions	1142:1152	arg1	DOM					1232:1234	the DOM	1228:1234	the DOM	1228:1234	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	7	72	theme	methyl	1186:1191	arg1	Me					1201:1202	Me	1201:1202	Me	1201:1202	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	7	72	theme	methyl	1186:1191	arg1	esters					1193:1198	fatty acid methyl esters	1175:1198	fatty acid methyl esters (Me)	1175:1203	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	13	73	theme	DOM	2121:2123	arg1	biodegradability					2101:2116	its biodegradability	2097:2116	its biodegradability of DOM	2097:2123	Collectively, FTCs tended to activate DOM and enhance its biodegradability of DOM, potentially hampering DOC accumulation and C sequestration.
36699583	8	74	theme	Me	1297:1298	arg1	abundance					1284:1292	only the relative abundance	1266:1292	only the relative abundance of Me in the 6FTCs treatment	1266:1321	In the subsuface soil layer, only the relative abundance of Me in the 6FTCs treatment increased significantly.
36699583	0	75	theme	boreal	105:110	arg1	forest					112:117	a boreal forest	103:117	a boreal forest during freeze-thaw cycles	103:143	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	5	76	theme	community	762:770	arg1	structure					772:780	microbial community structure	752:780	microbial community structure	752:780	Combined with pyrolysis gas chromatography-mass spectrometry and high-throughput sequencing techniques, the relationships between DOM chemodiversity and microbial community structure were assessed.
36699583	7	77	theme	phenols	1166:1172	arg1	proportions					1142:1152	the relative proportions	1129:1152	the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM	1129:1234	In the topsoil, FTCs significantly reduced the relative abundance of aromatic compounds, but increased the relative proportions of alkanes, phenols, fatty acid methyl esters (Me) and polysaccharides in the DOM.
36699583	8	78	from	abundance	1284:1292	arg1	treatment					1313:1321	the 6FTCs treatment	1303:1321	the 6FTCs treatment	1303:1321	In the subsuface soil layer, only the relative abundance of Me in the 6FTCs treatment increased significantly.
36699583	3	79	theme	chemical	401:408	arg1	composition					410:420	the chemical composition	397:420	the chemical composition of DOM molecules	397:437	However, the interactions between the chemical composition of DOM molecules and the microbial communities that drive C decomposition in the context of freeze-thaw are poorly understood.
36699583	0	80	theme	molecular	21:29	arg1	composition					31:41	the molecular composition	17:41	the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles	17:143	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	12	81	theme	other	1873:1877	arg1	Actinobacteria					1905:1918	Actinobacteria	1905:1918	Actinobacteria	1905:1918	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	81	theme	other	1873:1877	arg1	Thermoleophilia					1946:1960	Thermoleophilia	1946:1960	Thermoleophilia	1946:1960	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	81	theme	other	1873:1877	arg1	Alphaproteobacteria					1921:1939	Alphaproteobacteria	1921:1939	Alphaproteobacteria	1921:1939	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	12	81	theme	other	1873:1877	arg1	classes					1889:1895	other bacterial classes	1873:1895	other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia	1873:1960	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	14	82	theme	bacterial	2295:2303	arg1	communities					2305:2315	soil bacterial communities	2290:2315	soil bacterial communities	2290:2315	These findings highlight the potential of DOM molecular mechanisms to regulate the functional states of soil bacterial communities under increased FTCs.
36699583	12	83	from	compounds	2017:2025	arg1	topsoil					2034:2040	the topsoil	2030:2040	the topsoil	2030:2040	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	3	84	theme	molecules	429:437	arg1	composition					410:420	the chemical composition	397:420	the chemical composition of DOM molecules	397:437	However, the interactions between the chemical composition of DOM molecules and the microbial communities that drive C decomposition in the context of freeze-thaw are poorly understood.
36699583	3	84	theme	molecules	429:437	arg1	communities					457:467	the microbial communities	443:467	the microbial communities that drive C decomposition in the context of freeze-thaw	443:524	However, the interactions between the chemical composition of DOM molecules and the microbial communities that drive C decomposition in the context of freeze-thaw are poorly understood.
36699583	0	85	theme	organic	56:62	arg1	matter					64:69	dissolved organic matter	46:69	dissolved organic matter	46:69	Linkages between the molecular composition of dissolved organic matter and soil microbial community in a boreal forest during freeze-thaw cycles.
36699583	12	86	theme	labile	1825:1830	arg1	C					1832:1832	labile C	1825:1832	labile C	1825:1832	At class level, Gammaproteobacteria were significantly positively correlated with labile C (polysaccharides and alkanes), whereas other bacterial classes such as Actinobacteria, Alphaproteobacteria, and Thermoleophilia were significantly positively correlated with aromatic compounds in the topsoil.
36699583	11	87	theme	specific	1690:1697	arg1	microorganisms					1699:1712	specific microorganisms	1690:1712	specific microorganisms	1690:1712	Co-occurrence networks confirmed DOM-bacterial interactions, implying that specific microorganisms degrade specific substrates.
36699583	2	88	theme	forest	300:305	arg1	soils					307:311	boreal forest soils	293:311	boreal forest soils	293:311	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	1	89	theme	biogeochemical	204:217	arg1	processes					219:227	biogeochemical processes	204:227	biogeochemical processes	204:227	Soil dissolved organic matter (DOM) plays a vital role in biogeochemical processes.
36699583	10	90	theme	DOC	1610:1612	arg1	increment					1597:1605	the increment	1593:1605	the increment of DOC	1593:1612	Moreover, the relative abundance of these taxa was positively correlated with the increment of DOC.
36699583	2	91	theme	DOM	331:333	arg1	production					335:344	DOM production	331:344	DOM production	331:344	Global warming leads to increased freeze-thaw cycles (FTCs) in boreal forest soils, which can change DOM production and consumption.
36699583	9	92	theme	bacterial	1364:1372	arg1	communities					1374:1384	bacterial communities	1364:1384	bacterial communities	1364:1384	The response of bacterial communities to FTCs was more sensitive than that of fungi, among which only the relative abundance of Gammaproteobacteria increased by FTCs.
36455587	0	0	theme	Soybean	80:86	arg1	Matrices					88:95	Both Fermented Milk and Soybean Matrices	56:95	Matrices	88:95	Versatile Lactococcus lactis Strains Improve Texture in Both Fermented Milk and Soybean Matrices.
36455587	7	1	theme	gene	1105:1108	arg1	clusters					1110:1117	novel eps gene clusters	1095:1117	novel eps gene clusters	1095:1117	The comparative genomics approach revealed 10 texturing strains with novel eps gene clusters.
36455587	2	2	from	texture	381:387	arg1	milk					392:395	milk	392:395	milk	392:395	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	2	2	from	texture	381:387	arg1	matrices					416:423	liquid soybean matrices	401:423	liquid soybean matrices	401:423	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	3	3	theme	texturing	458:466	arg1	abilities					468:476	texturing abilities	458:476	texturing abilities in fermented milk	458:494	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	1	4	theme	fermented	207:215	arg1	milk					217:220	fermented milk	207:220	fermented milk	207:220	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	2	5	from	potential	268:276	arg1	fermentation					300:311	plant-based fermentation	288:311	plant-based fermentation	288:311	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	1	6	theme	milk	217:220	arg1	texture					196:202	texture	196:202	texture	196:202	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	1	6	theme	milk	217:220	arg1	taste					186:190	taste	186:190	taste	186:190	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	8	7	from	genes	1166:1170	arg1	milk					1199:1202	milk	1199:1202	milk	1199:1202	Currently, the relationship between the novel genes and their functionality in milk and plant matrices is unknown.
36455587	8	7	from	genes	1166:1170	arg1	matrices					1214:1221	plant matrices	1208:1221	plant matrices	1208:1221	Currently, the relationship between the novel genes and their functionality in milk and plant matrices is unknown.
36455587	7	8	theme	novel	1095:1099	arg1	clusters					1110:1117	novel eps gene clusters	1095:1117	novel eps gene clusters	1095:1117	The comparative genomics approach revealed 10 texturing strains with novel eps gene clusters.
36455587	3	9	from	abilities	468:476	arg1	milk					491:494	fermented milk	481:494	fermented milk	481:494	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	1	10	used	used	140:143	arg2	bacteria					110:117	Lactic acid bacteria	98:117	Lactic acid bacteria (LAB)	98:123	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	1	10	used	used	140:143	arg2	LAB					120:122	LAB	120:122	LAB	120:122	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	6	11	theme	strains	965:971	arg1	sequences					942:950	whole genome sequences	929:950	whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters	929:1023	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36455587	7	12	theme	eps	1101:1103	arg1	clusters					1110:1117	novel eps gene clusters	1095:1117	novel eps gene clusters	1095:1117	The comparative genomics approach revealed 10 texturing strains with novel eps gene clusters.
36455587	6	13	theme	texturing	955:963	arg1	strains					965:971	texturing strains	955:971	texturing strains	955:971	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36455587	8	14	theme	novel	1160:1164	arg1	genes					1166:1170	the novel genes	1156:1170	the novel genes	1156:1170	Currently, the relationship between the novel genes and their functionality in milk and plant matrices is unknown.
36455587	5	15	theme	enhanced	885:892	arg1	texture					894:900	the enhanced texture	881:900	the enhanced texture in both matrices	881:917	It appeared as if it was the properties of the polysaccharides rather than their protein interaction partners that were responsible for the enhanced texture in both matrices.
36455587	2	16	theme	lactis	362:367	arg1	strains					369:375	1232 Lactococcus lactis strains	345:375	1232 Lactococcus lactis strains	345:375	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	6	17	theme	polysaccharide	977:990	arg1	clusters					1016:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36455587	0	18	theme	Versatile	0:8	arg1	Strains					29:35	Versatile Lactococcus lactis Strains	0:35	Versatile Lactococcus lactis Strains	0:35	Versatile Lactococcus lactis Strains Improve Texture in Both Fermented Milk and Soybean Matrices.
36455587	2	19	theme	Lactococcus	350:360	arg1	strains					369:375	1232 Lactococcus lactis strains	345:375	1232 Lactococcus lactis strains	345:375	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	0	20	theme	Lactococcus	10:20	arg1	Strains					29:35	Versatile Lactococcus lactis Strains	0:35	Versatile Lactococcus lactis Strains	0:35	Versatile Lactococcus lactis Strains Improve Texture in Both Fermented Milk and Soybean Matrices.
36455587	3	21	theme	fermented	481:489	arg1	milk					491:494	fermented milk	481:494	fermented milk	481:494	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	3	22	with	strains	445:451	arg1	abilities					468:476	texturing abilities	458:476	texturing abilities in fermented milk	458:494	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	3	23	from	differences	579:589	arg1	composition					594:604	composition	594:604	composition of the two matrices	594:624	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	4	24	theme	Exo-cellular	627:638	arg1	production					655:664	Exo-cellular polysaccharide production	627:664	Exo-cellular polysaccharide production	627:664	Exo-cellular polysaccharide production is believed to contribute positively to fermented milk and plant-base texture.
36455587	2	25	theme	liquid	401:406	arg1	matrices					416:423	liquid soybean matrices	401:423	liquid soybean matrices	401:423	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	7	26	theme	texturing	1072:1080	arg1	strains					1082:1088	10 texturing strains	1069:1088	10 texturing strains with novel eps gene clusters	1069:1117	The comparative genomics approach revealed 10 texturing strains with novel eps gene clusters.
36455587	2	27	theme	texturing	258:266	arg1	potential					268:276	the texturing potential	254:276	the texturing potential of LAB in plant-based fermentation	254:311	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	1	28	theme	shelf	159:163	arg1	life					165:168	the shelf life	155:168	the shelf life	155:168	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	4	29	theme	fermented	706:714	arg1	milk					716:719	fermented milk	706:719	fermented milk	706:719	Exo-cellular polysaccharide production is believed to contribute positively to fermented milk and plant-base texture.
36455587	5	30	from	texture	894:900	arg1	matrices					910:917	both matrices	905:917	both matrices	905:917	It appeared as if it was the properties of the polysaccharides rather than their protein interaction partners that were responsible for the enhanced texture in both matrices.
36455587	1	31	theme	Lactic	98:103	arg1	LAB					120:122	LAB	120:122	LAB	120:122	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	1	31	theme	Lactic	98:103	arg1	bacteria					110:117	Lactic acid bacteria	98:117	Lactic acid bacteria (LAB)	98:123	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	5	32	theme	polysaccharides	792:806	arg1	it					763:764	it	763:764	it	763:764	It appeared as if it was the properties of the polysaccharides rather than their protein interaction partners that were responsible for the enhanced texture in both matrices.
36455587	5	32	theme	polysaccharides	792:806	arg1	properties					774:783	the properties	770:783	the properties of the polysaccharides	770:806	It appeared as if it was the properties of the polysaccharides rather than their protein interaction partners that were responsible for the enhanced texture in both matrices.
36455587	0	33	theme	lactis	22:27	arg1	Strains					29:35	Versatile Lactococcus lactis Strains	0:35	Versatile Lactococcus lactis Strains	0:35	Versatile Lactococcus lactis Strains Improve Texture in Both Fermented Milk and Soybean Matrices.
36455587	6	34	theme	genome	935:940	arg1	sequences					942:950	whole genome sequences	929:950	whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters	929:1023	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36455587	1	35	theme	acid	105:108	arg1	LAB					120:122	LAB	120:122	LAB	120:122	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	1	35	theme	acid	105:108	arg1	bacteria					110:117	Lactic acid bacteria	98:117	Lactic acid bacteria (LAB)	98:123	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	5	36	theme	interaction	834:844	arg1	partners					846:853	their protein interaction partners	820:853	their protein interaction partners that were responsible for the enhanced texture in both matrices	820:917	It appeared as if it was the properties of the polysaccharides rather than their protein interaction partners that were responsible for the enhanced texture in both matrices.
36455587	2	37	theme	soybean	408:414	arg1	matrices					416:423	liquid soybean matrices	401:423	liquid soybean matrices	401:423	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	7	38	theme	comparative	1030:1040	arg1	approach					1051:1058	The comparative genomics approach	1026:1058	The comparative genomics approach	1026:1058	The comparative genomics approach revealed 10 texturing strains with novel eps gene clusters.
36455587	6	39	theme	whole	929:933	arg1	sequences					942:950	whole genome sequences	929:950	whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters	929:1023	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36455587	5	40	theme	protein	826:832	arg1	partners					846:853	their protein interaction partners	820:853	their protein interaction partners that were responsible for the enhanced texture in both matrices	820:917	It appeared as if it was the properties of the polysaccharides rather than their protein interaction partners that were responsible for the enhanced texture in both matrices.
36455587	7	41	theme	genomics	1042:1049	arg1	approach					1051:1058	The comparative genomics approach	1026:1058	The comparative genomics approach	1026:1058	The comparative genomics approach revealed 10 texturing strains with novel eps gene clusters.
36455587	1	42	dep	taste	186:190	arg1	the					182:184	the	182:184	the	182:184	Lactic acid bacteria (LAB) have long been used to extend the shelf life and improve the taste and texture of fermented milk.
36455587	2	43	theme	strains	369:375	arg1	screening					332:340	high-throughput screening	316:340	high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices	316:423	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	8	44	theme	plant	1208:1212	arg1	matrices					1214:1221	plant matrices	1208:1221	plant matrices	1208:1221	Currently, the relationship between the novel genes and their functionality in milk and plant matrices is unknown.
36455587	6	45	theme	gene	1011:1014	arg1	clusters					1016:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36455587	2	46	theme	plant-based	288:298	arg1	fermentation					300:311	plant-based fermentation	288:311	plant-based fermentation	288:311	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	3	47	theme	fermented	542:550	arg1	soybean					552:558	fermented soybean	542:558	fermented soybean	542:558	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	3	48	theme	large	573:577	arg1	differences					579:589	the large differences	569:589	the large differences in composition of the two matrices	569:624	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	4	49	theme	polysaccharide	640:653	arg1	production					655:664	Exo-cellular polysaccharide production	627:664	Exo-cellular polysaccharide production	627:664	Exo-cellular polysaccharide production is believed to contribute positively to fermented milk and plant-base texture.
36455587	2	50	theme	LAB	281:283	arg1	potential					268:276	the texturing potential	254:276	the texturing potential of LAB in plant-based fermentation	254:311	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	4	51	theme	plant-base	725:734	arg1	texture					736:742	plant-base texture	725:742	plant-base texture	725:742	Exo-cellular polysaccharide production is believed to contribute positively to fermented milk and plant-base texture.
36455587	3	52	theme	most	440:443	arg1	strains					445:451	most strains	440:451	most strains with texturing abilities in fermented milk	440:494	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	6	53	theme	eps	1006:1008	arg1	clusters					1016:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36455587	3	54	theme	matrices	617:624	arg1	composition					594:604	composition	594:604	composition of the two matrices	594:624	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	3	55	from	texture	531:537	arg1	soybean					552:558	fermented soybean	542:558	fermented soybean	542:558	We found that most strains with texturing abilities in fermented milk were also capable of enhancing the texture in fermented soybean, despite the large differences in composition of the two matrices.
36455587	7	56	with	strains	1082:1088	arg1	clusters					1110:1117	novel eps gene clusters	1095:1117	novel eps gene clusters	1095:1117	The comparative genomics approach revealed 10 texturing strains with novel eps gene clusters.
36455587	0	57	theme	Fermented	61:69	arg1	Milk					71:74	Both Fermented Milk and Soybean Matrices	56:95	Milk	71:74	Versatile Lactococcus lactis Strains Improve Texture in Both Fermented Milk and Soybean Matrices.
36455587	8	58	from	functionality	1182:1194	arg1	milk					1199:1202	milk	1199:1202	milk	1199:1202	Currently, the relationship between the novel genes and their functionality in milk and plant matrices is unknown.
36455587	8	58	from	functionality	1182:1194	arg1	matrices					1214:1221	plant matrices	1208:1221	plant matrices	1208:1221	Currently, the relationship between the novel genes and their functionality in milk and plant matrices is unknown.
36455587	2	59	theme	high-throughput	316:330	arg1	screening					332:340	high-throughput screening	316:340	high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices	316:423	In this study, we investigated the texturing potential of LAB in plant-based fermentation by high-throughput screening of 1232 Lactococcus lactis strains for texture in milk and liquid soybean matrices.
36455587	6	60	theme	biosynthesis	992:1003	arg1	clusters					1016:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	polysaccharide biosynthesis (eps) gene clusters	977:1023	We mined whole genome sequences of texturing strains for polysaccharide biosynthesis (eps) gene clusters.
36408150	6	0	theme	deviation	962:970	arg1	values					972:977	The relative standard deviation values	940:977	The relative standard deviation values for intra-day and inter-day precision	940:1015	The relative standard deviation values for intra-day and inter-day precision were ≤ 5.49%.
36408150	11	1	theme	human	1630:1634	arg1	serum					1636:1640	the human serum	1626:1640	the human serum	1626:1640	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	1	2	theme	Altered	154:160	arg1	levels					169:174	Altered glycan levels	154:174	Altered glycan levels in serum	154:183	Altered glycan levels in serum have been associated with increased risk of cancer.
36408150	6	3	theme	relative	944:951	arg1	values					972:977	The relative standard deviation values	940:977	The relative standard deviation values for intra-day and inter-day precision	940:1015	The relative standard deviation values for intra-day and inter-day precision were ≤ 5.49%.
36408150	0	4	theme	HPLC	74:77	arg1	method					79:84	an improved HPLC method	62:84	an improved HPLC method	62:84	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	10	5	theme	serum	1370:1374	arg1	levels					1391:1396	the serum monosaccharide levels	1366:1396	the serum monosaccharide levels	1366:1396	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	10	5	theme	serum	1370:1374	arg1	higher					1417:1422	higher	1417:1422	higher	1417:1422	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	1	6	theme	glycan	162:167	arg1	levels					169:174	Altered glycan levels	154:174	Altered glycan levels in serum	154:183	Altered glycan levels in serum have been associated with increased risk of cancer.
36408150	0	7	from	Determination	0:12	arg1	serum					53:57	human serum	47:57	human serum	47:57	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	11	8	theme	simple	1512:1517	arg1	method					1562:1567	a simple, reliable, low-cost, and reproducible HPLC method	1510:1567	a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer	1510:1721	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	4	9	theme	solvent	747:753	arg1	A					755:755	solvent A	747:755	solvent A	747:755	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	9	theme	solvent	747:753	arg1	NH4Ac-HAc					728:736	NH4Ac-HAc	728:736	NH4Ac-HAc	728:736	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	11	10	theme	screening	1683:1691	arg1	marker					1693:1698	a screening marker	1681:1698	a screening marker for endometrial cancer	1681:1721	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	4	11	dep	buffer	720:725	arg1	PH=5.5					739:744	PH=5.5	739:744	PH=5.5	739:744	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	11	dep	buffer	720:725	arg1	A					755:755	solvent A	747:755	solvent A	747:755	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	11	dep	buffer	720:725	arg1	NH4Ac-HAc					728:736	NH4Ac-HAc	728:736	NH4Ac-HAc	728:736	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	11	12	theme	HPLC	1557:1560	arg1	method					1562:1567	a simple, reliable, low-cost, and reproducible HPLC method	1510:1567	a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer	1510:1721	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	9	13	with	controls	1320:1327	arg1	cancer					1290:1295	endometrial cancer	1278:1295	endometrial cancer	1278:1295	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	4	14	theme	100	696:698	arg1	mM					700:701	mM	700:701	mM	700:701	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	0	15	from	serum	53:57	arg1	Determination					0:12	Determination	0:12	Determination of monosaccharide composition in human serum by an improved HPLC method	0:84	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	0	15	from	serum	53:57	arg1	application					94:104	its application	90:104	its application as candidate biomarkers for endometrial cancer	90:151	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	6	16	theme	inter-day	997:1005	arg1	precision					1007:1015	inter-day precision	997:1015	inter-day precision	997:1015	The relative standard deviation values for intra-day and inter-day precision were ≤ 5.49%.
36408150	5	17	theme	R2	928:929	arg1	0.995					933:937	R2 > 0.995	928:937	R2 > 0.995	928:937	The calibration standard curves for the eight monosaccharides showed good linearity over the range of 2.5-500μg/mL with R2 > 0.995.
36408150	5	18	theme	calibration	812:822	arg1	curves					833:838	The calibration standard curves	808:838	The calibration standard curves for the eight monosaccharides	808:868	The calibration standard curves for the eight monosaccharides showed good linearity over the range of 2.5-500μg/mL with R2 > 0.995.
36408150	2	19	theme	Glucuronic	382:391	arg1	D-mannose					343:351	D-mannose	343:351	D-mannose	343:351	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	19	theme	Glucuronic	382:391	arg1	acid					393:396	Glucuronic acid	382:396	Glucuronic acid	382:396	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	4	20	with	separation	680:689	arg1	acetonitrile					759:770	acetonitrile	759:770	acetonitrile (ACN, solvent B)	759:787	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	20	with	separation	680:689	arg1	buffer					720:725	100 mM ammonium acetate buffer	696:725	100 mM ammonium acetate buffer	696:725	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	2	21	theme	human	446:450	arg1	serum					452:456	human serum	446:456	human serum	446:456	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	5	22	theme	>	931:931	arg1	0.995					933:937	R2 > 0.995	928:937	R2 > 0.995	928:937	The calibration standard curves for the eight monosaccharides showed good linearity over the range of 2.5-500μg/mL with R2 > 0.995.
36408150	8	23	theme	separate	1134:1141	arg1	monosaccharides					1143:1157	separate monosaccharides	1134:1157	separate monosaccharides from serum	1134:1168	We observed that this column exhibited high specificity and selectivity to separate monosaccharides from serum.
36408150	8	24	from	serum	1164:1168	arg1	monosaccharides					1143:1157	separate monosaccharides	1134:1157	separate monosaccharides from serum	1134:1168	We observed that this column exhibited high specificity and selectivity to separate monosaccharides from serum.
36408150	10	25	theme	healthy	1450:1456	arg1	P					1468:1468	P value< 0.0001	1468:1482	P value< 0.0001	1468:1482	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	10	25	theme	healthy	1450:1456	arg1	controls					1458:1465	healthy controls	1450:1465	healthy controls (P value< 0.0001)	1450:1483	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	11	26	theme	low-cost	1530:1537	arg1	method					1562:1567	a simple, reliable, low-cost, and reproducible HPLC method	1510:1567	a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer	1510:1721	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	3	27	theme	hydrolyzed	539:548	arg1	samples					556:562	hydrolyzed serum samples	539:562	hydrolyzed serum samples	539:562	Monosaccharides obtained from hydrolyzed serum samples were derivatized by 1-Phenyl-3-methyl-5-pyrazolone.
36408150	4	28	dep	acetonitrile	759:770	arg1	ACN					773:775	ACN	773:775	ACN	773:775	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	28	dep	acetonitrile	759:770	arg1	B					786:786	solvent B	778:786	solvent B	778:786	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	10	29	theme	value<	1470:1475	arg1	P					1468:1468	P value< 0.0001	1468:1482	P value< 0.0001	1468:1482	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	10	29	theme	value<	1470:1475	arg1	controls					1458:1465	healthy controls	1450:1465	healthy controls (P value< 0.0001)	1450:1483	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	4	30	theme	mobile	794:799	arg1	phase					801:805	a mobile phase	792:805	a mobile phase	792:805	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	30	theme	mobile	794:799	arg1	column					633:638	A ZORBAX XDB-C18 column	616:638	A ZORBAX XDB-C18 column(150×4.6mm)	616:649	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	3	31	theme	serum	550:554	arg1	samples					556:562	hydrolyzed serum samples	539:562	hydrolyzed serum samples	539:562	Monosaccharides obtained from hydrolyzed serum samples were derivatized by 1-Phenyl-3-methyl-5-pyrazolone.
36408150	5	32	theme	standard	824:831	arg1	curves					833:838	The calibration standard curves	808:838	The calibration standard curves for the eight monosaccharides	808:868	The calibration standard curves for the eight monosaccharides showed good linearity over the range of 2.5-500μg/mL with R2 > 0.995.
36408150	0	33	theme	composition	32:42	arg1	Determination					0:12	Determination	0:12	Determination of monosaccharide composition in human serum by an improved HPLC method	0:84	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	0	33	theme	composition	32:42	arg1	application					94:104	its application	90:104	its application as candidate biomarkers for endometrial cancer	90:151	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	10	34	theme	monosaccharide	1376:1389	arg1	levels					1391:1396	the serum monosaccharide levels	1366:1396	the serum monosaccharide levels	1366:1396	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	10	34	theme	monosaccharide	1376:1389	arg1	higher					1417:1422	higher	1417:1422	higher	1417:1422	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	2	35	dep	composition	330:340	arg1	D-galactose					410:420	D-galactose	410:420	D-galactose	410:420	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	35	dep	composition	330:340	arg1	D-xylose					423:430	D-xylose	423:430	D-xylose	423:430	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	35	dep	composition	330:340	arg1	D-mannose					343:351	D-mannose	343:351	D-mannose	343:351	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	35	dep	composition	330:340	arg1	acid					393:396	Glucuronic acid	382:396	Glucuronic acid	382:396	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	35	dep	composition	330:340	arg1	Glucosamine					354:364	Glucosamine	354:364	Glucosamine	354:364	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	35	dep	composition	330:340	arg1	D-glucose					399:407	D-glucose	399:407	D-glucose	399:407	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	35	dep	composition	330:340	arg1	L-fucose					433:440	L-fucose	433:440	L-fucose	433:440	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	35	dep	composition	330:340	arg1	Galactosamine					367:379	Galactosamine	367:379	Galactosamine	367:379	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	2	36	theme	HPLC-based	286:295	arg1	method					297:302	a HPLC-based method	284:302	a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose	284:473	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	8	37	theme	high	1098:1101	arg1	specificity					1103:1113	high specificity	1098:1113	high specificity	1098:1113	We observed that this column exhibited high specificity and selectivity to separate monosaccharides from serum.
36408150	0	38	theme	candidate	109:117	arg1	biomarkers					119:128	candidate biomarkers	109:128	candidate biomarkers for endometrial cancer	109:151	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	11	39	theme	potential	1653:1661	arg1	value					1663:1667	potential value	1653:1667	potential value	1653:1667	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	11	40	dep	separation	1577:1586	arg1	monosaccharides					1607:1621	monosaccharides	1607:1621	monosaccharides	1607:1621	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	11	40	dep	separation	1577:1586	arg1	the					1573:1575	the	1573:1575	the	1573:1575	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	4	41	theme	XDB-C18	625:631	arg1	phase					801:805	a mobile phase	792:805	a mobile phase	792:805	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	41	theme	XDB-C18	625:631	arg1	150×4.6mm					640:648	150×4.6mm	640:648	150×4.6mm	640:648	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	41	theme	XDB-C18	625:631	arg1	column					633:638	A ZORBAX XDB-C18 column	616:638	A ZORBAX XDB-C18 column(150×4.6mm)	616:649	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	0	42	theme	human	47:51	arg1	serum					53:57	human serum	47:57	human serum	47:57	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	4	43	theme	mM	700:701	arg1	acetonitrile					759:770	acetonitrile	759:770	acetonitrile (ACN, solvent B)	759:787	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	43	theme	mM	700:701	arg1	buffer					720:725	100 mM ammonium acetate buffer	696:725	100 mM ammonium acetate buffer	696:725	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	0	44	theme	endometrial	134:144	arg1	cancer					146:151	endometrial cancer	134:151	endometrial cancer	134:151	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	4	45	theme	solvent	778:784	arg1	ACN					773:775	ACN	773:775	ACN	773:775	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	45	theme	solvent	778:784	arg1	B					786:786	solvent B	778:786	solvent B	778:786	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	11	46	from	separation	1577:1586	arg1	serum					1636:1640	the human serum	1626:1640	the human serum	1626:1640	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	9	47	theme	matched	1304:1310	arg1	controls					1320:1327	30 matched healthy controls	1301:1327	30 matched healthy controls	1301:1327	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	11	48	from	quantification	1592:1605	arg1	serum					1636:1640	the human serum	1626:1640	the human serum	1626:1640	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	4	49	theme	ZORBAX	618:623	arg1	phase					801:805	a mobile phase	792:805	a mobile phase	792:805	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	49	theme	ZORBAX	618:623	arg1	150×4.6mm					640:648	150×4.6mm	640:648	150×4.6mm	640:648	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	49	theme	ZORBAX	618:623	arg1	column					633:638	A ZORBAX XDB-C18 column	616:638	A ZORBAX XDB-C18 column(150×4.6mm)	616:649	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	50	used	used	655:658	arg2	phase					801:805	a mobile phase	792:805	a mobile phase	792:805	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	50	used	used	655:658	arg2	150×4.6mm					640:648	150×4.6mm	640:648	150×4.6mm	640:648	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	50	used	used	655:658	arg2	column					633:638	A ZORBAX XDB-C18 column	616:638	A ZORBAX XDB-C18 column(150×4.6mm)	616:649	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	1	51	theme	increased	211:219	arg1	risk					221:224	increased risk	211:224	increased risk of cancer	211:234	Altered glycan levels in serum have been associated with increased risk of cancer.
36408150	9	52	theme	endometrial	1278:1288	arg1	cancer					1290:1295	endometrial cancer	1278:1295	endometrial cancer	1278:1295	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	4	53	theme	ammonium	703:710	arg1	acetonitrile					759:770	acetonitrile	759:770	acetonitrile (ACN, solvent B)	759:787	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	53	theme	ammonium	703:710	arg1	buffer					720:725	100 mM ammonium acetate buffer	696:725	100 mM ammonium acetate buffer	696:725	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	9	54	from	levels	1251:1256	arg1	patients					1264:1271	30 patients	1261:1271	30 patients with endometrial cancer	1261:1295	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	9	54	from	levels	1251:1256	arg1	controls					1320:1327	30 matched healthy controls	1301:1327	30 matched healthy controls	1301:1327	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	9	55	theme	monosaccharide	1236:1249	arg1	levels					1251:1256	the serum monosaccharide levels	1226:1256	the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls	1226:1327	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	11	56	theme	reproducible	1544:1555	arg1	method					1562:1567	a simple, reliable, low-cost, and reproducible HPLC method	1510:1567	a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer	1510:1721	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	2	57	theme	monosaccharide	315:328	arg1	composition					330:340	monosaccharide composition	315:340	monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose)	315:441	In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard.
36408150	11	58	theme	reliable	1520:1527	arg1	method					1562:1567	a simple, reliable, low-cost, and reproducible HPLC method	1510:1567	a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer	1510:1721	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	0	59	from	application	94:104	arg1	serum					53:57	human serum	47:57	human serum	47:57	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	9	60	theme	healthy	1312:1318	arg1	controls					1320:1327	30 matched healthy controls	1301:1327	30 matched healthy controls	1301:1327	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	5	61	theme	good	877:880	arg1	linearity					882:890	good linearity	877:890	good linearity	877:890	The calibration standard curves for the eight monosaccharides showed good linearity over the range of 2.5-500μg/mL with R2 > 0.995.
36408150	5	62	theme	2.5-500μg/mL	910:921	arg1	range					901:905	the range	897:905	the range of 2.5-500μg/mL	897:921	The calibration standard curves for the eight monosaccharides showed good linearity over the range of 2.5-500μg/mL with R2 > 0.995.
36408150	4	63	theme	acetate	712:718	arg1	acetonitrile					759:770	acetonitrile	759:770	acetonitrile (ACN, solvent B)	759:787	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	4	63	theme	acetate	712:718	arg1	buffer					720:725	100 mM ammonium acetate buffer	696:725	100 mM ammonium acetate buffer	696:725	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	9	64	with	patients	1264:1271	arg1	cancer					1290:1295	endometrial cancer	1278:1295	endometrial cancer	1278:1295	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	11	65	theme	endometrial	1704:1714	arg1	cancer					1716:1721	endometrial cancer	1704:1721	endometrial cancer	1704:1721	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	0	66	theme	improved	65:72	arg1	method					79:84	an improved HPLC method	62:84	an improved HPLC method	62:84	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	9	67	theme	serum	1230:1234	arg1	levels					1251:1256	the serum monosaccharide levels	1226:1256	the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls	1226:1327	This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls.
36408150	10	68	theme	Statistical	1330:1340	arg1	analysis					1342:1349	Statistical analysis	1330:1349	Statistical analysis	1330:1349	Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001).
36408150	1	69	theme	cancer	229:234	arg1	risk					221:224	increased risk	211:224	increased risk of cancer	211:234	Altered glycan levels in serum have been associated with increased risk of cancer.
36408150	0	70	from	composition	32:42	arg1	serum					53:57	human serum	47:57	human serum	47:57	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	0	71	theme	monosaccharide	17:30	arg1	composition					32:42	monosaccharide composition	17:42	monosaccharide composition in human serum by an improved HPLC method	17:84	Determination of monosaccharide composition in human serum by an improved HPLC method and its application as candidate biomarkers for endometrial cancer.
36408150	1	72	from	levels	169:174	arg1	serum					179:183	serum	179:183	serum	179:183	Altered glycan levels in serum have been associated with increased risk of cancer.
36408150	4	73	theme	chromatographic	664:678	arg1	separation					680:689	chromatographic separation	664:689	chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B)	664:787	A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase.
36408150	11	74	contain	has	1649:1651	arg2	value					1663:1667	potential value	1653:1667	potential value	1653:1667	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	11	74	contain	has	1649:1651	arg1	serum					1636:1640	the human serum	1626:1640	the human serum	1626:1640	Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.
36408150	6	75	theme	standard	953:960	arg1	values					972:977	The relative standard deviation values	940:977	The relative standard deviation values for intra-day and inter-day precision	940:1015	The relative standard deviation values for intra-day and inter-day precision were ≤ 5.49%.
35598809	0	0	theme	intensified	79:89	arg1	approach					105:112	An intensified clarification approach	76:112	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.	0:139	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	3	1	theme	disk	502:505	arg1	centrifugation					513:526	disk stack centrifugation	502:526	disk stack centrifugation	502:526	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	7	2	theme	high	1238:1241	arg1	%					1263:1263	> 91%	1259:1263	> 91%	1259:1263	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	2	theme	high	1238:1241	arg1	recoveries					1247:1256	high mAb recoveries	1238:1256	high mAb recoveries (> 91%)	1238:1264	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	4	3	theme	FBC	861:863	arg1	separation					866:875	a mild fluidized bed centrifuge (FBC) separation	828:875	a mild fluidized bed centrifuge (FBC) separation	828:875	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	1	4	theme	improved	194:201	arg1	clarification					203:215	improved clarification	194:215	improved clarification of cell culture fluids (CCF)	194:244	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	1	5	theme	monoclonal	277:286	arg1	mAb					300:302	mAb	300:302	mAb	300:302	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	1	5	theme	monoclonal	277:286	arg1	antibodies					288:297	monoclonal antibodies	277:297	monoclonal antibodies (mAb)	277:303	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	6	6	theme	FBC	1113:1115	arg1	throughput					1117:1126	similar FBC throughput	1105:1126	similar FBC throughput	1105:1126	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	7	7	theme	high	1299:1302	arg1	removals					1308:1315	high DNA removals	1299:1315	high DNA removals (> 91%)	1299:1323	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	7	theme	high	1299:1302	arg1	%					1322:1322	> 91%	1318:1322	> 91%	1318:1322	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	6	8	theme	filter	1189:1194	arg1	times					1218:1222	the maximum filter throughput up to four times	1177:1222	the maximum filter throughput up to four times	1177:1222	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	1	9	theme	antibodies	288:297	arg1	production					263:272	the production	259:272	the production of monoclonal antibodies (mAb)	259:303	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	0	10	theme	clarification	91:103	arg1	approach					105:112	An intensified clarification approach	76:112	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.	0:139	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	9	11	theme	CCF	1500:1502	arg1	pretreatments					1504:1516	both CCF pretreatments	1495:1516	both CCF pretreatments using a FBC clarification approach	1495:1551	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	7	12	theme	>	1259:1259	arg1	%					1263:1263	> 91%	1259:1263	> 91%	1259:1263	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	12	theme	>	1259:1259	arg1	recoveries					1247:1256	high mAb recoveries	1238:1256	high mAb recoveries (> 91%)	1238:1264	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	4	13	theme	high	697:700	arg1	level					702:706	a high level	695:706	a high level of impurities	695:720	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	4	14	theme	impurities	711:720	arg1	level					702:706	a high level	695:706	a high level of impurities	695:720	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	0	15	theme	monoclonal	118:127	arg1	antibodies					129:138	monoclonal antibodies	118:138	monoclonal antibodies	118:138	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	9	16	theme	great	1435:1439	arg1	potential					1441:1449	a great potential	1433:1449	a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach	1433:1551	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	4	17	theme	bed	845:847	arg1	separation					866:875	a mild fluidized bed centrifuge (FBC) separation	828:875	a mild fluidized bed centrifuge (FBC) separation	828:875	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	2	18	dep	damage	370:375	arg1	cells					377:381	cells	377:381	cells	377:381	However, such pretreatments pose the risks to alter the mAb and damage cells.
35598809	3	19	theme	clarification	439:451	arg1	process					453:459	the subsequent clarification process	424:459	the subsequent clarification process	424:459	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	4	20	theme	fluidized	835:843	arg1	separation					866:875	a mild fluidized bed centrifuge (FBC) separation	828:875	a mild fluidized bed centrifuge (FBC) separation	828:875	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	4	21	theme	cationic	772:779	arg1	each					803:806	each	803:806	each	803:806	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	4	21	theme	cationic	772:779	arg1	flocculation					789:800	cationic polymer flocculation	772:800	cationic polymer flocculation	772:800	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	5	22	from	effects	992:998	arg1	composition					1020:1030	CCF composition	1016:1030	CCF composition	1016:1030	Therefore, low-viable CCF´s were pretreated and characterized to investigate the effects of additives on CCF composition and stability.
35598809	5	22	from	effects	992:998	arg1	stability					1036:1044	stability	1036:1044	stability	1036:1044	Therefore, low-viable CCF´s were pretreated and characterized to investigate the effects of additives on CCF composition and stability.
35598809	4	23	theme	cultures	658:665	arg1	clarification					623:635	the clarification	619:635	the clarification particularly of cell cultures with low viability	619:684	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	0	24	theme	bed	10:12	arg1	centrifugation					14:27	Fluidized bed centrifugation	0:27	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.	0:139	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	6	25	theme	maximum	1181:1187	arg1	times					1218:1222	the maximum filter throughput up to four times	1177:1222	the maximum filter throughput up to four times	1177:1222	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	2	26	dep	risks	343:347	arg1	alter					352:356	alter	352:356	to alter the mAb	349:364	However, such pretreatments pose the risks to alter the mAb and damage cells.
35598809	2	26	dep	risks	343:347	arg1	damage					370:375	damage	370:375	damage	370:375	However, such pretreatments pose the risks to alter the mAb and damage cells.
35598809	6	27	theme	similar	1105:1111	arg1	throughput					1117:1126	similar FBC throughput	1105:1126	similar FBC throughput	1105:1126	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	1	28	theme	cell	220:223	arg1	CCF					241:243	CCF	241:243	CCF	241:243	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	1	28	theme	cell	220:223	arg1	fluids					233:238	cell culture fluids	220:238	cell culture fluids (CCF)	220:244	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	0	29	theme	Fluidized	0:8	arg1	centrifugation					14:27	Fluidized bed centrifugation	0:27	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.	0:139	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	4	30	theme	centrifuge	849:858	arg1	separation					866:875	a mild fluidized bed centrifuge (FBC) separation	828:875	a mild fluidized bed centrifuge (FBC) separation	828:875	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	3	31	theme	impurities	565:574	arg1	release					544:550	a release	542:550	a release of host cell impurities	542:574	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	1	32	theme	culture	225:231	arg1	CCF					241:243	CCF	241:243	CCF	241:243	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	1	32	theme	culture	225:231	arg1	fluids					233:238	cell culture fluids	220:238	cell culture fluids (CCF)	220:244	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	5	33	theme	low-viable	922:931	arg1	CCF´s					933:937	low-viable CCF´s	922:937	low-viable CCF´s	922:937	Therefore, low-viable CCF´s were pretreated and characterized to investigate the effects of additives on CCF composition and stability.
35598809	2	34	theme	such	315:318	arg1	pretreatments					320:332	such pretreatments	315:332	such pretreatments	315:332	However, such pretreatments pose the risks to alter the mAb and damage cells.
35598809	8	35	theme	Similar	1340:1346	arg1	profiles					1355:1362	Similar glycan profiles	1340:1362	Similar glycan profiles	1340:1362	Similar glycan profiles and dimer ratios suggest consistent mAb quality.
35598809	1	36	theme	fluids	233:238	arg1	clarification					203:215	improved clarification	194:215	improved clarification of cell culture fluids (CCF)	194:244	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	9	37	theme	FBC	1526:1528	arg1	approach					1544:1551	a FBC clarification approach	1524:1551	a FBC clarification approach	1524:1551	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	4	38	theme	polymer	781:787	arg1	each					803:806	each	803:806	each	803:806	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	4	38	theme	polymer	781:787	arg1	flocculation					789:800	cationic polymer flocculation	772:800	cationic polymer flocculation	772:800	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	8	39	theme	mAb	1400:1402	arg1	quality					1404:1410	consistent mAb quality	1389:1410	consistent mAb quality	1389:1410	Similar glycan profiles and dimer ratios suggest consistent mAb quality.
35598809	3	40	theme	host	555:558	arg1	impurities					565:574	host cell impurities	555:574	host cell impurities	555:574	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	6	41	theme	clarification	1050:1062	arg1	experiments					1064:1074	clarification experiments	1050:1074	clarification experiments	1050:1074	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	6	42	theme	untreated	1143:1151	arg1	reference					1153:1161	an untreated reference	1140:1161	an untreated reference	1140:1161	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	8	43	theme	consistent	1389:1398	arg1	quality					1404:1410	consistent mAb quality	1389:1410	consistent mAb quality	1389:1410	Similar glycan profiles and dimer ratios suggest consistent mAb quality.
35598809	5	44	theme	CCF	1016:1018	arg1	composition					1020:1030	CCF composition	1016:1030	CCF composition	1016:1030	Therefore, low-viable CCF´s were pretreated and characterized to investigate the effects of additives on CCF composition and stability.
35598809	3	45	theme	subsequent	428:437	arg1	process					453:459	the subsequent clarification process	424:459	the subsequent clarification process	424:459	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	4	46	theme	cell	653:656	arg1	cultures					658:665	cell cultures	653:665	cell cultures	653:665	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	0	47	theme	precipitated	32:43	arg1	cultures					66:73	precipitated and flocculated cell cultures	32:73	precipitated and flocculated cell cultures	32:73	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	0	48	theme	cell	61:64	arg1	cultures					66:73	precipitated and flocculated cell cultures	32:73	precipitated and flocculated cell cultures	32:73	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	4	49	theme	subsequent	883:892	arg1	step					905:908	a subsequent filtration step	881:908	a subsequent filtration step	881:908	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	4	50	theme	mild	830:833	arg1	separation					866:875	a mild fluidized bed centrifuge (FBC) separation	828:875	a mild fluidized bed centrifuge (FBC) separation	828:875	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	9	51	contain	have	1428:1431	arg1	findings					1419:1426	These findings	1413:1426	These findings	1413:1426	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	9	51	contain	have	1428:1431	arg2	potential					1441:1449	a great potential	1433:1449	a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach	1433:1551	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	7	52	theme	low	1267:1269	arg1	NTU					1290:1292	< 3.1 NTU	1284:1292	< 3.1 NTU	1284:1292	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	52	theme	low	1267:1269	arg1	turbidities					1271:1281	low turbidities	1267:1281	low turbidities (< 3.1 NTU)	1267:1293	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	0	53	theme	flocculated	49:59	arg1	cultures					66:73	precipitated and flocculated cell cultures	32:73	precipitated and flocculated cell cultures	32:73	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	8	54	theme	glycan	1348:1353	arg1	profiles					1355:1362	Similar glycan profiles	1340:1362	Similar glycan profiles	1340:1362	Similar glycan profiles and dimer ratios suggest consistent mAb quality.
35598809	3	55	theme	stack	507:511	arg1	centrifugation					513:526	disk stack centrifugation	502:526	disk stack centrifugation	502:526	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	4	56	theme	low	672:674	arg1	viability					676:684	low viability	672:684	low viability	672:684	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	3	57	theme	cell	560:563	arg1	impurities					565:574	host cell impurities	555:574	host cell impurities	555:574	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	8	58	theme	dimer	1368:1372	arg1	ratios					1374:1379	dimer ratios	1368:1379	dimer ratios	1368:1379	Similar glycan profiles and dimer ratios suggest consistent mAb quality.
35598809	7	59	theme	>	1318:1318	arg1	removals					1308:1315	high DNA removals	1299:1315	high DNA removals (> 91%)	1299:1323	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	59	theme	>	1318:1318	arg1	%					1322:1322	> 91%	1318:1322	> 91%	1318:1322	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	60	theme	DNA	1304:1306	arg1	removals					1308:1315	high DNA removals	1299:1315	high DNA removals (> 91%)	1299:1323	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	60	theme	DNA	1304:1306	arg1	%					1322:1322	> 91%	1318:1322	> 91%	1318:1322	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	1	61	theme	Precipitation	141:153	arg1	pretreatments					172:184	Precipitation and flocculation pretreatments	141:184	Precipitation and flocculation pretreatments	141:184	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	4	62	theme	low-pH	747:752	arg1	precipitation					754:766	low-pH precipitation	747:766	low-pH precipitation	747:766	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	4	63	theme	filtration	894:903	arg1	step					905:908	a subsequent filtration step	881:908	a subsequent filtration step	881:908	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	0	64	dep	centrifugation	14:27	arg1	approach					105:112	An intensified clarification approach	76:112	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.	0:139	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	3	65	theme	shear	482:486	arg1	forces					488:493	high shear forces	477:493	high shear forces during disk stack centrifugation	477:526	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	3	66	theme	high	477:480	arg1	forces					488:493	high shear forces	477:493	high shear forces during disk stack centrifugation	477:526	This can be additionally exacerbated by the subsequent clarification process, for example by high shear forces during disk stack centrifugation, resulting in a release of host cell impurities.
35598809	7	67	theme	mAb	1243:1245	arg1	%					1263:1263	> 91%	1259:1263	> 91%	1259:1263	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	7	67	theme	mAb	1243:1245	arg1	recoveries					1247:1256	high mAb recoveries	1238:1256	high mAb recoveries (> 91%)	1238:1264	Furthermore, high mAb recoveries (> 91%), low turbidities (< 3.1 NTU) and high DNA removals (> 91%) were achieved.
35598809	4	68	with	clarification	623:635	arg1	viability					676:684	low viability	672:684	low viability	672:684	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	5	69	theme	additives	1003:1011	arg1	effects					992:998	the effects	988:998	the effects of additives on CCF composition and stability	988:1044	Therefore, low-viable CCF´s were pretreated and characterized to investigate the effects of additives on CCF composition and stability.
35598809	9	70	theme	mAb	1464:1466	arg1	processing					1479:1488	mAb downstream processing	1464:1488	mAb downstream processing	1464:1488	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	1	71	theme	flocculation	159:170	arg1	pretreatments					172:184	Precipitation and flocculation pretreatments	141:184	Precipitation and flocculation pretreatments	141:184	Precipitation and flocculation pretreatments promise improved clarification of cell culture fluids (CCF) to intensify the production of monoclonal antibodies (mAb).
35598809	6	72	theme	throughput	1196:1205	arg1	times					1218:1222	the maximum filter throughput up to four times	1177:1222	the maximum filter throughput up to four times	1177:1222	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	6	73	dep	four	1213:1216	arg1	to					1210:1211	to	1210:1211	to	1210:1211	In clarification experiments, both pretreatments achieved similar FBC throughput compared to an untreated reference but increased the maximum filter throughput up to four times.
35598809	0	74	theme	cultures	66:73	arg1	centrifugation					14:27	Fluidized bed centrifugation	0:27	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.	0:139	Fluidized bed centrifugation of precipitated and flocculated cell cultures: An intensified clarification approach for monoclonal antibodies.
35598809	9	75	theme	clarification	1530:1542	arg1	approach					1544:1551	a FBC clarification approach	1524:1551	a FBC clarification approach	1524:1551	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	9	76	theme	downstream	1468:1477	arg1	processing					1479:1488	mAb downstream processing	1464:1488	mAb downstream processing	1464:1488	These findings have a great potential to intensify mAb downstream processing with both CCF pretreatments using a FBC clarification approach.
35598809	4	77	with	combination	811:821	arg1	step					905:908	a subsequent filtration step	881:908	a subsequent filtration step	881:908	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
35598809	4	77	with	combination	811:821	arg1	separation					866:875	a mild fluidized bed centrifuge (FBC) separation	828:875	a mild fluidized bed centrifuge (FBC) separation	828:875	To overcome these limitations and enhance the clarification particularly of cell cultures with low viability and thus a high level of impurities, this study investigated low-pH precipitation and cationic polymer flocculation, each in combination with a mild fluidized bed centrifuge (FBC) separation and a subsequent filtration step.
37100317	10	0	from	different	1615:1623	arg1	freshwater					1628:1637	freshwater	1628:1637	freshwater	1628:1637	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	10	0	from	different	1615:1623	arg1	molluscs					1650:1657	marine molluscs	1643:1657	marine molluscs	1643:1657	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	7	1	theme	key	1126:1128	arg1	roles					1130:1134	key roles	1126:1134	key roles	1126:1134	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	2	2	theme	carbonate	370:378	arg1	deposition					380:389	the calcium carbonate deposition	358:389	the calcium carbonate deposition during shell mineralization	358:417	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	1	3	theme	biomaterial	258:268	arg1	synthesis					270:278	shell-inspired biomaterial synthesis	243:278	shell-inspired biomaterial synthesis	243:278	Uncovering the molecular mechanism of shell formation not only reveals the evolution of molluscs but also lay a foundation for shell-inspired biomaterial synthesis.
37100317	9	4	from	shells	1443:1448	arg1	absent					1422:1427	absent	1422:1427	absent	1422:1427	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	10	5	from	freshwater	1628:1637	arg1	different					1615:1623	different	1615:1623	different	1615:1623	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	9	6	theme	freshwater	1467:1476	arg1	Gastropods					1478:1487	freshwater Gastropods	1467:1487	freshwater Gastropods	1467:1487	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	2	7	theme	calcium	362:368	arg1	deposition					380:389	the calcium carbonate deposition	358:389	the calcium carbonate deposition during shell mineralization	358:417	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	4	8	theme	shell	601:605	arg1	proteins					607:614	shell proteins	601:614	shell proteins	601:614	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	0	9	from	Characterization	0:15	arg1	snails					57:62	two freshwater snails	42:62	two freshwater snails	42:62	Characterization of the shell proteins in two freshwater snails Pomacea canaliculata and Cipangopaludina chinensis.
37100317	2	10	theme	key	304:306	arg1	proteins					287:294	Shell proteins	281:294	Shell proteins	281:294	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	2	10	theme	key	304:306	arg1	macromolecules					308:321	the key macromolecules	300:321	the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied	300:456	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	9	11	theme	snail	1437:1441	arg1	shells					1443:1448	both snail shells	1432:1448	both snail shells	1432:1448	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	9	12	theme	carbonic	1399:1406	arg1	anhydrase					1408:1416	carbonic anhydrase	1399:1416	carbonic anhydrase	1399:1416	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	10	13	from	molluscs	1650:1657	arg1	different					1615:1623	different	1615:1623	different	1615:1623	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	3	14	theme	marine	535:540	arg1	species					542:548	marine species	535:548	marine species	535:548	However, previous studies on shell biomineralization have mainly focused on marine species.
37100317	4	15	from	proteins	607:614	arg1	species					674:680	an alien species	665:680	an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	665:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	15	from	proteins	607:614	arg1	canaliculata					643:654	the apple snail Pomacea canaliculata	619:654	the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	619:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	16	dep	microstructure	582:595	arg1	the					578:580	the	578:580	the	578:580	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	7	17	theme	differential	1160:1171	arg1	components					1201:1210	immune components	1194:1210	immune components	1194:1210	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	17	theme	differential	1160:1171	arg1	proteins					1173:1180	the differential proteins	1156:1180	the differential proteins	1156:1180	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	10	18	theme	more	1696:1699	arg1	attention					1701:1709	more attention	1696:1709	more attention	1696:1709	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	7	19	theme	immune	1194:1199	arg1	components					1201:1210	immune components	1194:1210	immune components	1194:1210	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	19	theme	immune	1194:1199	arg1	proteins					1173:1180	the differential proteins	1156:1180	the differential proteins	1156:1180	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	8	20	from	presence	1217:1224	arg1	matrices					1250:1257	shell matrices	1244:1257	shell matrices	1244:1257	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	8	20	from	presence	1217:1224	arg1	domains					1282:1288	the chitin-binding domains	1263:1288	the chitin-binding domains containing PcSP6/CcSP9	1263:1311	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	9	21	contain	have	1495:1498	arg2	pathways					1507:1514	unique pathways	1500:1514	unique pathways	1500:1514	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	9	21	contain	have	1495:1498	arg1	Gastropods					1478:1487	freshwater Gastropods	1467:1487	freshwater Gastropods	1467:1487	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	10	22	theme	comprehensive	1755:1767	arg1	insight					1769:1775	a more comprehensive insight	1748:1775	a more comprehensive insight into biomineralization	1748:1798	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	3	23	theme	previous	468:475	arg1	studies					477:483	previous studies	468:483	previous studies on shell biomineralization	468:510	However, previous studies on shell biomineralization have mainly focused on marine species.
37100317	5	24	theme	shell	817:821	arg1	microstructures					823:837	the shell microstructures	813:837	the shell microstructures	813:837	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	5	24	theme	shell	817:821	arg1	similar					844:850	similar	844:850	similar	844:850	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	0	25	from	proteins	30:37	arg1	snails					57:62	two freshwater snails	42:62	two freshwater snails	42:62	Characterization of the shell proteins in two freshwater snails Pomacea canaliculata and Cipangopaludina chinensis.
37100317	2	26	theme	organic	330:336	arg1	matrices					338:345	the organic matrices	326:345	the organic matrices	326:345	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	5	27	from	similar	844:850	arg1	snails					865:870	these two snails	855:870	these two snails	855:870	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	5	28	theme	shell	877:881	arg1	matrix					883:888	the shell matrix	873:888	the shell matrix in C. chinensis	873:904	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	4	29	from	microstructure	582:595	arg1	species					674:680	an alien species	665:680	an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	665:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	29	from	microstructure	582:595	arg1	canaliculata					643:654	the apple snail Pomacea canaliculata	619:654	the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	619:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	10	30	theme	marine	1643:1648	arg1	molluscs					1650:1657	marine molluscs	1643:1657	marine molluscs	1643:1657	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	0	31	dep	canaliculata	72:83	arg1	Characterization					0:15	Characterization	0:15	Characterization of the shell proteins in two freshwater snails	0:62	Characterization of the shell proteins in two freshwater snails Pomacea canaliculata and Cipangopaludina chinensis.
37100317	2	32	theme	shell	398:402	arg1	mineralization					404:417	shell mineralization	398:417	shell mineralization	398:417	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	0	33	theme	shell	24:28	arg1	proteins					30:37	the shell proteins	20:37	the shell proteins in two freshwater snails	20:62	Characterization of the shell proteins in two freshwater snails Pomacea canaliculata and Cipangopaludina chinensis.
37100317	4	34	dep	snail	722:726	arg1	chinensis					744:752	chinensis	744:752	chinensis	744:752	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	3	35	theme	shell	488:492	arg1	biomineralization					494:510	shell biomineralization	488:510	shell biomineralization	488:510	However, previous studies on shell biomineralization have mainly focused on marine species.
37100317	5	36	from	matrix	883:888	arg1	chinensis					896:904	chinensis	896:904	chinensis	896:904	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	6	37	theme	proteins	974:981	arg1	compositions					952:963	the compositions	948:963	the compositions of shell proteins	948:981	Moreover, the compositions of shell proteins were quite different.
37100317	6	37	theme	proteins	974:981	arg1	different					994:1002	different	994:1002	different	994:1002	Moreover, the compositions of shell proteins were quite different.
37100317	7	38	theme	proline-rich	1082:1093	arg1	protein					1095:1101	proline-rich protein	1082:1101	proline-rich protein	1082:1101	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	8	39	theme	chitin	1342:1347	arg1	fraction					1360:1367	a major fraction	1352:1367	a major fraction in gastropods	1352:1381	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	8	39	theme	chitin	1342:1347	arg1	relevance					1329:1337	the relevance	1325:1337	the relevance of chitin	1325:1347	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	9	40	theme	calcification	1532:1544	arg1	process					1546:1552	the calcification process	1528:1552	the calcification process	1528:1552	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	4	41	theme	freshwater	711:720	arg1	native					763:768	native	763:768	native	763:768	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	41	theme	freshwater	711:720	arg1	snail					722:726	a freshwater snail	709:726	a freshwater snail Cipangopaludina chinensis which is native to China	709:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	6	42	theme	shell	968:972	arg1	proteins					974:981	shell proteins	968:981	shell proteins	968:981	Moreover, the compositions of shell proteins were quite different.
37100317	8	43	theme	shell	1244:1248	arg1	matrices					1250:1257	shell matrices	1244:1257	shell matrices	1244:1257	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	4	44	theme	alien	668:672	arg1	species					674:680	an alien species	665:680	an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	665:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	44	theme	alien	668:672	arg1	canaliculata					643:654	the apple snail Pomacea canaliculata	619:654	the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	619:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	0	45	theme	proteins	30:37	arg1	Characterization					0:15	Characterization	0:15	Characterization of the shell proteins in two freshwater snails	0:62	Characterization of the shell proteins in two freshwater snails Pomacea canaliculata and Cipangopaludina chinensis.
37100317	3	46	from	studies	477:483	arg1	biomineralization					494:510	shell biomineralization	488:510	shell biomineralization	488:510	However, previous studies on shell biomineralization have mainly focused on marine species.
37100317	8	47	theme	chitin-binding	1267:1280	arg1	domains					1282:1288	the chitin-binding domains	1263:1288	the chitin-binding domains containing PcSP6/CcSP9	1263:1311	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	0	48	theme	freshwater	46:55	arg1	snails					57:62	two freshwater snails	42:62	two freshwater snails	42:62	Characterization of the shell proteins in two freshwater snails Pomacea canaliculata and Cipangopaludina chinensis.
37100317	8	49	theme	major	1354:1358	arg1	fraction					1360:1367	a major fraction	1352:1367	a major fraction in gastropods	1352:1381	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	8	49	theme	major	1354:1358	arg1	relevance					1329:1337	the relevance	1325:1337	the relevance of chitin	1325:1347	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	0	50	from	snails	57:62	arg1	Characterization					0:15	Characterization	0:15	Characterization of the shell proteins in two freshwater snails	0:62	Characterization of the shell proteins in two freshwater snails Pomacea canaliculata and Cipangopaludina chinensis.
37100317	5	51	from	snails	865:870	arg1	microstructures					823:837	the shell microstructures	813:837	the shell microstructures	813:837	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	5	51	from	snails	865:870	arg1	similar					844:850	similar	844:850	similar	844:850	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	5	52	theme	more	916:919	arg1	polysaccharides					921:935	more polysaccharides	916:935	more polysaccharides	916:935	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	7	53	theme	shell	1139:1143	arg1	formation					1145:1153	shell formation	1139:1153	shell formation	1139:1153	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	9	54	from	absent	1422:1427	arg1	shells					1443:1448	both snail shells	1432:1448	both snail shells	1432:1448	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	10	55	theme	freshwater	1718:1727	arg1	species					1729:1735	the freshwater species	1714:1735	the freshwater species	1714:1735	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	7	56	theme	shared	1015:1020	arg1	PcSP6/CcSP9					1051:1061	PcSP6/CcSP9	1051:1061	PcSP6/CcSP9	1051:1061	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	56	theme	shared	1015:1020	arg1	protein					1095:1101	proline-rich protein	1082:1101	proline-rich protein	1082:1101	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	56	theme	shared	1015:1020	arg1	Calmodulin-A					1064:1075	Calmodulin-A	1064:1075	Calmodulin-A	1064:1075	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	56	theme	shared	1015:1020	arg1	proteins					1031:1038	the shared 12 shell proteins	1011:1038	the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein)	1011:1102	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	1	57	theme	molecular	131:139	arg1	mechanism					141:149	the molecular mechanism	127:149	the molecular mechanism of shell formation	127:168	Uncovering the molecular mechanism of shell formation not only reveals the evolution of molluscs but also lay a foundation for shell-inspired biomaterial synthesis.
37100317	8	58	contain	containing	1290:1299	arg2	PcSP6/CcSP9					1301:1311	PcSP6/CcSP9	1301:1311	PcSP6/CcSP9	1301:1311	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	8	58	contain	containing	1290:1299	arg1	domains					1282:1288	the chitin-binding domains	1263:1288	the chitin-binding domains containing PcSP6/CcSP9	1263:1311	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	2	59	theme	Shell	281:285	arg1	proteins					287:294	Shell proteins	281:294	Shell proteins	281:294	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	2	59	theme	Shell	281:285	arg1	macromolecules					308:321	the key macromolecules	300:321	the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied	300:456	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	8	60	attach	presence	1217:1224	arg2	chitin					1229:1234	chitin	1229:1234	chitin	1229:1234	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	8	60	attach	presence	1217:1224	arg1	matrices					1250:1257	shell matrices	1244:1257	shell matrices	1244:1257	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	8	60	attach	presence	1217:1224	arg1	domains					1282:1288	the chitin-binding domains	1263:1288	the chitin-binding domains containing PcSP6/CcSP9	1263:1311	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	8	61	from	fraction	1360:1367	arg1	gastropods					1372:1381	gastropods	1372:1381	gastropods	1372:1381	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	4	62	theme	snail	629:633	arg1	species					674:680	an alien species	665:680	an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	665:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	62	theme	snail	629:633	arg1	canaliculata					643:654	the apple snail Pomacea canaliculata	619:654	the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	619:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	7	63	theme	shell	1025:1029	arg1	PcSP6/CcSP9					1051:1061	PcSP6/CcSP9	1051:1061	PcSP6/CcSP9	1051:1061	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	63	theme	shell	1025:1029	arg1	protein					1095:1101	proline-rich protein	1082:1101	proline-rich protein	1082:1101	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	63	theme	shell	1025:1029	arg1	Calmodulin-A					1064:1075	Calmodulin-A	1064:1075	Calmodulin-A	1064:1075	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	7	63	theme	shell	1025:1029	arg1	proteins					1031:1038	the shared 12 shell proteins	1011:1038	the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein)	1011:1102	While the shared 12 shell proteins (including PcSP6/CcSP9, Calmodulin-A, and proline-rich protein) were supposed to play key roles in shell formation, the differential proteins were mainly immune components.
37100317	5	64	contain	contained	906:914	arg2	polysaccharides					921:935	more polysaccharides	916:935	more polysaccharides	916:935	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	5	64	contain	contained	906:914	arg1	matrix					883:888	the shell matrix	873:888	the shell matrix in C. chinensis	873:904	The results showed that although the shell microstructures were similar in these two snails, the shell matrix in C. chinensis contained more polysaccharides.
37100317	2	65	theme	matrices	338:345	arg1	proteins					287:294	Shell proteins	281:294	Shell proteins	281:294	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	2	65	theme	matrices	338:345	arg1	macromolecules					308:321	the key macromolecules	300:321	the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied	300:456	Shell proteins are the key macromolecules of the organic matrices that guide the calcium carbonate deposition during shell mineralization and have thus been intensively studied.
37100317	4	66	theme	apple	623:627	arg1	species					674:680	an alien species	665:680	an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	665:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	66	theme	apple	623:627	arg1	canaliculata					643:654	the apple snail Pomacea canaliculata	619:654	the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	619:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	10	67	theme	shell	1580:1584	arg1	mineralization					1586:1599	shell mineralization	1580:1599	shell mineralization	1580:1599	Our study suggested that shell mineralization might be very different in freshwater and marine molluscs, and therefore, the field should pay more attention to the freshwater species to achieve a more comprehensive insight into biomineralization.
37100317	9	68	theme	unique	1500:1505	arg1	pathways					1507:1514	unique pathways	1500:1514	unique pathways	1500:1514	Interestingly, carbonic anhydrase was absent in both snail shells, suggesting that freshwater Gastropods might have unique pathways to regulate the calcification process.
37100317	1	69	theme	molluscs	204:211	arg1	evolution					191:199	the evolution	187:199	the evolution of molluscs	187:211	Uncovering the molecular mechanism of shell formation not only reveals the evolution of molluscs but also lay a foundation for shell-inspired biomaterial synthesis.
37100317	1	70	theme	shell	154:158	arg1	formation					160:168	shell formation	154:168	shell formation	154:168	Uncovering the molecular mechanism of shell formation not only reveals the evolution of molluscs but also lay a foundation for shell-inspired biomaterial synthesis.
37100317	8	71	theme	chitin	1229:1234	arg1	presence					1217:1224	The presence	1213:1224	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9	1213:1311	The presence of chitin in both shell matrices and the chitin-binding domains containing PcSP6/CcSP9 underpinned the relevance of chitin as a major fraction in gastropods.
37100317	4	72	theme	Pomacea	635:641	arg1	species					674:680	an alien species	665:680	an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	665:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	4	72	theme	Pomacea	635:641	arg1	canaliculata					643:654	the apple snail Pomacea canaliculata	619:654	the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China	619:777	In this study, we compared the microstructure and shell proteins in the apple snail Pomacea canaliculata which is an alien species that has invaded Asia, and a freshwater snail Cipangopaludina chinensis which is native to China.
37100317	1	73	theme	formation	160:168	arg1	mechanism					141:149	the molecular mechanism	127:149	the molecular mechanism of shell formation	127:168	Uncovering the molecular mechanism of shell formation not only reveals the evolution of molluscs but also lay a foundation for shell-inspired biomaterial synthesis.
37100317	1	74	theme	shell-inspired	243:256	arg1	synthesis					270:278	shell-inspired biomaterial synthesis	243:278	shell-inspired biomaterial synthesis	243:278	Uncovering the molecular mechanism of shell formation not only reveals the evolution of molluscs but also lay a foundation for shell-inspired biomaterial synthesis.
34611872	1	0	theme	O-linked	171:178	arg1	glycosylation					180:192	O-linked glycosylation	171:192	O-linked glycosylation	171:192	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	4	1	theme	knocking	524:531	arg1	effects					513:519	The effects	509:519	The effects of knocking out different sets of enzyme activities	509:571	The effects of knocking out different sets of enzyme activities can be compared.
34611872	1	2	theme	glycosylation	180:192	arg1	enzymes					160:166	the biosynthetic enzymes	143:166	the biosynthetic enzymes of O-linked glycosylation	143:192	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	5	3	from	mucin	714:718	arg1	example					726:732	an example	723:732	an example	723:732	A method is provided for predicting the enzymes required to produce a given substrate, using an O-glycan from human gastric mucin as an example.
34611872	5	3	from	mucin	714:718	arg1	O-glycan					686:693	an O-glycan	683:693	an O-glycan from human gastric mucin	683:718	A method is provided for predicting the enzymes required to produce a given substrate, using an O-glycan from human gastric mucin as an example.
34611872	5	4	theme	given	660:664	arg1	substrate					666:674	a given substrate	658:674	a given substrate	658:674	A method is provided for predicting the enzymes required to produce a given substrate, using an O-glycan from human gastric mucin as an example.
34611872	6	5	theme	enzymes	797:803	arg1	systems					772:778	other systems	766:778	other systems of glycosylation enzymes	766:803	The system has been adapted to other systems of glycosylation enzymes, and an application to ganglioside oligosaccharide synthesis is demonstrated.
34611872	1	6	theme	online	122:127	arg1	simulation					129:138	an online simulation	119:138	an online simulation of the biosynthetic enzymes of O-linked glycosylation	119:192	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	6	7	theme	glycosylation	783:795	arg1	enzymes					797:803	glycosylation enzymes	783:803	glycosylation enzymes	783:803	The system has been adapted to other systems of glycosylation enzymes, and an application to ganglioside oligosaccharide synthesis is demonstrated.
34611872	1	8	theme	web	81:83	arg1	O-Glycologue					97:108	The web application O-Glycologue	77:108	The web application O-Glycologue	77:108	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	3	9	theme	resulting	452:460	arg1	SBML					503:506	SBML	503:506	SBML	503:506	The resulting networks of reactions can be exported as SBML.
34611872	3	9	theme	resulting	452:460	arg1	networks					462:469	The resulting networks	448:469	The resulting networks of reactions	448:482	The resulting networks of reactions can be exported as SBML.
34611872	1	10	theme	application	85:95	arg1	O-Glycologue					97:108	The web application O-Glycologue	77:108	The web application O-Glycologue	77:108	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	6	11	theme	other	766:770	arg1	systems					772:778	other systems	766:778	other systems of glycosylation enzymes	766:803	The system has been adapted to other systems of glycosylation enzymes, and an application to ganglioside oligosaccharide synthesis is demonstrated.
34611872	4	12	theme	activities	562:571	arg1	sets					547:550	different sets	537:550	different sets of enzyme activities	537:571	The effects of knocking out different sets of enzyme activities can be compared.
34611872	4	12	theme	activities	562:571	arg1	activities					562:571	enzyme activities	555:571	enzyme activities	555:571	The effects of knocking out different sets of enzyme activities can be compared.
34611872	1	13	theme	knowledge-based	203:217	arg1	system					219:224	a knowledge-based system	201:224	a knowledge-based system described previously	201:245	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	0	14	theme	Formal-Language-Based	16:36	arg1	Generator					38:46	A Formal-Language-Based Generator	14:46	O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.	0:75	O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.
34611872	4	15	theme	enzyme	555:560	arg1	activities					562:571	enzyme activities	555:571	enzyme activities	555:571	The effects of knocking out different sets of enzyme activities can be compared.
34611872	1	16	link	O-linked	171:178	arg1	glycosylation					180:192	O-linked glycosylation	171:192	O-linked glycosylation	171:192	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	2	17	theme	condensed	318:326	arg1	Glycans					248:254	Glycans	248:254	Glycans	248:254	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	2	17	theme	condensed	318:326	arg1	names					328:332	IUPAC condensed names	312:332	IUPAC condensed names	312:332	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	5	18	theme	human	700:704	arg1	mucin					714:718	human gastric mucin	700:718	human gastric mucin	700:718	A method is provided for predicting the enzymes required to produce a given substrate, using an O-glycan from human gastric mucin as an example.
34611872	2	19	theme	IUPAC	312:316	arg1	Glycans					248:254	Glycans	248:254	Glycans	248:254	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	2	19	theme	IUPAC	312:316	arg1	names					328:332	IUPAC condensed names	312:332	IUPAC condensed names	312:332	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	5	20	theme	gastric	706:712	arg1	mucin					714:718	human gastric mucin	700:718	human gastric mucin	700:718	A method is provided for predicting the enzymes required to produce a given substrate, using an O-glycan from human gastric mucin as an example.
34611872	4	21	theme	different	537:545	arg1	sets					547:550	different sets	537:550	different sets of enzyme activities	537:571	The effects of knocking out different sets of enzyme activities can be compared.
34611872	4	21	theme	different	537:545	arg1	activities					562:571	enzyme activities	555:571	enzyme activities	555:571	The effects of knocking out different sets of enzyme activities can be compared.
34611872	6	22	theme	oligosaccharide	840:854	arg1	synthesis					856:864	ganglioside oligosaccharide synthesis	828:864	ganglioside oligosaccharide synthesis	828:864	The system has been adapted to other systems of glycosylation enzymes, and an application to ganglioside oligosaccharide synthesis is demonstrated.
34611872	2	23	theme	regular-expression-based	397:420	arg1	substitutions					422:434	regular-expression-based substitutions	397:434	regular-expression-based substitutions on strings	397:445	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	2	23	theme	regular-expression-based	397:420	arg1	enzymes					367:373	the enzymes	363:373	the enzymes	363:373	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	0	24	theme	Networks	67:74	arg1	Generator					38:46	A Formal-Language-Based Generator	14:46	O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.	0:75	O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.
34611872	6	25	theme	ganglioside	828:838	arg1	synthesis					856:864	ganglioside oligosaccharide synthesis	828:864	ganglioside oligosaccharide synthesis	828:864	The system has been adapted to other systems of glycosylation enzymes, and an application to ganglioside oligosaccharide synthesis is demonstrated.
34611872	7	26	from	//glycologue.org/o/	918:936	arg1	available					899:907	available	899:907	available	899:907	O-Glycologue is available at https://glycologue.org/o/ .
34611872	0	27	theme	O-Glycosylation	51:65	arg1	Networks					67:74	O-Glycosylation Networks	51:74	O-Glycosylation Networks	51:74	O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.
34611872	7	28	theme	https	912:916	arg1	//glycologue.org/o/					918:936	https://glycologue.org/o/	912:936	https://glycologue.org/o/	912:936	O-Glycologue is available at https://glycologue.org/o/ .
34611872	2	29	theme	condensed	283:291	arg1	format					293:298	GlycoCT condensed format	275:298	GlycoCT condensed format	275:298	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	2	30	from	substitutions	422:434	arg1	strings					439:445	strings	439:445	strings	439:445	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	1	31	theme	biosynthetic	147:158	arg1	enzymes					160:166	the biosynthetic enzymes	143:166	the biosynthetic enzymes of O-linked glycosylation	143:192	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	3	32	theme	reactions	474:482	arg1	SBML					503:506	SBML	503:506	SBML	503:506	The resulting networks of reactions can be exported as SBML.
34611872	3	32	theme	reactions	474:482	arg1	networks					462:469	The resulting networks	448:469	The resulting networks of reactions	448:482	The resulting networks of reactions can be exported as SBML.
34611872	2	33	theme	GlycoCT	275:281	arg1	format					293:298	GlycoCT condensed format	275:298	GlycoCT condensed format	275:298	Glycans can be imported in GlycoCT condensed format, or else as IUPAC condensed names, and passed as substrates to the enzymes, which are modeled as regular-expression-based substitutions on strings.
34611872	1	34	theme	enzymes	160:166	arg1	simulation					129:138	an online simulation	119:138	an online simulation of the biosynthetic enzymes of O-linked glycosylation	119:192	The web application O-Glycologue provides an online simulation of the biosynthetic enzymes of O-linked glycosylation, using a knowledge-based system described previously.
34611872	0	35	dep	O-Glycologue	0:11	arg1	Generator					38:46	A Formal-Language-Based Generator	14:46	O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.	0:75	O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.
35967821	4	0	from	Glc	494:496	arg1	ratio					509:513	a molar ratio	501:513	a molar ratio of 44.6:55.4, and CWP-0.2	501:539	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	4	1	theme	10.6:23.3:5.2:4.9:56	605:624	arg1	ratio					596:600	a molar ratio	588:600	a molar ratio of 10.6:23.3:5.2:4.9:56	588:624	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	4	2	from	Rha	557:559	arg1	ratio					596:600	a molar ratio	588:600	a molar ratio of 10.6:23.3:5.2:4.9:56	588:624	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	0	3	from	characterization	11:26	arg1	Cicer					108:112	Cicer	108:112	Cicer	108:112	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	3	from	characterization	11:26	arg1	seeds					128:132	chickpeas (Cicer arietinum L.) seeds	97:132	chickpeas (Cicer arietinum L.) seeds	97:132	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	6	4	theme	RAW	1073:1075	arg1	macrophages					1083:1093	RAW 264.7 macrophages	1073:1093	RAW 264.7 macrophages	1073:1093	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	5	5	theme	2,4	796:798	arg1	-α-l-Rhap-					800:809	→ 2,4)-α-l-Rhap-	794:809	→ 2,4)-α-l-Rhap-	794:809	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	5	theme	2,4	796:798	arg1	connection					756:765	the main chain connection	741:765	the main chain connection of CWP-0.2	741:776	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	4	6	theme	CWP-0.2	533:539	arg1	ratio					509:513	a molar ratio	501:513	a molar ratio of 44.6:55.4, and CWP-0.2	501:539	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	3	7	theme	chemical	318:325	arg1	structure					327:335	The chemical structure	314:335	The chemical structure of the two polysaccharides	314:362	The chemical structure of the two polysaccharides was characterized by various methods.
35967821	5	8	theme	→	813:813	arg1	-α-d-Galp-					817:826	1 → 3)-α-d-Galp-	811:826	1 → 3)-α-d-Galp-	811:826	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	9	theme	→	794:794	arg1	-α-l-Rhap-					800:809	→ 2,4)-α-l-Rhap-	794:809	→ 2,4)-α-l-Rhap-	794:809	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	9	theme	→	794:794	arg1	connection					756:765	the main chain connection	741:765	the main chain connection of CWP-0.2	741:776	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	6	10	theme	antioxidant	919:929	arg1	activity					952:959	antioxidant and immunoregulatory activity	919:959	antioxidant and immunoregulatory activity	919:959	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	5	11	theme	CWP	707:709	arg1	connection					693:702	the main chain connection	678:702	the main chain connection of CWP	678:709	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	12	theme	main	682:685	arg1	chain					687:691	the main chain	678:691	the main chain connection of CWP	678:709	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	0	13	theme	Structural	0:9	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	4	14	from	Gal	581:583	arg1	ratio					596:600	a molar ratio	588:600	a molar ratio of 10.6:23.3:5.2:4.9:56	588:624	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	2	15	dep	Cicer	287:291	arg1	L.					303:304	Cicer arietinum L.	287:304	Cicer arietinum L.	287:304	37 × 105 Da) and CWP-0.2 (1.58 × 104 Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds.
35967821	0	16	from	evaluation	44:53	arg1	Cicer					108:112	Cicer	108:112	Cicer	108:112	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	16	from	evaluation	44:53	arg1	seeds					128:132	chickpeas (Cicer arietinum L.) seeds	97:132	chickpeas (Cicer arietinum L.) seeds	97:132	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	5	17	theme	chain	687:691	arg1	connection					693:702	the main chain connection	678:702	the main chain connection of CWP	678:709	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	0	18	dep	Cicer	108:112	arg1	L.					124:125	Cicer arietinum L.	108:125	Cicer arietinum L.	108:125	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	5	19	theme	CWP-0.2	770:776	arg1	-α-l-Rhap-					800:809	→ 2,4)-α-l-Rhap-	794:809	→ 2,4)-α-l-Rhap-	794:809	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	19	theme	CWP-0.2	770:776	arg1	connection					756:765	the main chain connection	741:765	the main chain connection of CWP-0.2	741:776	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	3	20	theme	polysaccharides	348:362	arg1	structure					327:335	The chemical structure	314:335	The chemical structure of the two polysaccharides	314:362	The chemical structure of the two polysaccharides was characterized by various methods.
35967821	4	21	theme	44.6:55.4	518:526	arg1	ratio					509:513	a molar ratio	501:513	a molar ratio of 44.6:55.4, and CWP-0.2	501:539	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	6	22	contain	had	915:917	arg2	activity					952:959	antioxidant and immunoregulatory activity	919:959	antioxidant and immunoregulatory activity	919:959	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	6	22	contain	had	915:917	arg1	CWP-0.2					907:913	CWP-0.2	907:913	CWP-0.2	907:913	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	6	22	contain	had	915:917	arg1	CWP					899:901	CWP	899:901	CWP	899:901	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	5	23	dep	-α-d-Galp-	817:826	arg1	o-4					880:882	-α-l-Rhap-(1 → o-4	865:882	-α-l-Rhap-(1 → o-4	865:882	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	23	dep	-α-d-Galp-	817:826	arg1	→					830:830	1 →	828:830	1 → with the branched chain of → 2,4	828:863	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	23	dep	-α-d-Galp-	817:826	arg1	3					815:815	3	815:815	3	815:815	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	7	24	theme	higher	1127:1132	arg1	bioactivity					1134:1144	significantly higher bioactivity	1113:1144	significantly higher bioactivity	1113:1144	CWP-0.2 revealed significantly higher bioactivity than CWP.
35967821	0	25	theme	bioactivity	32:42	arg1	evaluation					44:53	bioactivity evaluation	32:53	bioactivity evaluation	32:53	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	5	26	theme	n	732:732	arg1	→					734:734	→ (2-β-d-Fruf-1) n →	715:734	→ (2-β-d-Fruf-1) n →	715:734	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	4	27	from	Glc	572:574	arg1	ratio					596:600	a molar ratio	588:600	a molar ratio of 10.6:23.3:5.2:4.9:56	588:624	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	5	28	theme	Further	627:633	arg1	characterization					646:661	Further structural characterization	627:661	Further structural characterization	627:661	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	4	29	theme	methylation	433:443	arg1	analysis					445:452	methylation analysis	433:452	methylation analysis	433:452	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	6	30	from	MCP-1	1064:1068	arg1	macrophages					1083:1093	RAW 264.7 macrophages	1073:1093	RAW 264.7 macrophages	1073:1093	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	5	31	with	→	830:830	arg1	chain					850:854	the branched chain	837:854	the branched chain of → 2,4	837:863	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	32	theme	structural	635:644	arg1	characterization					646:661	Further structural characterization	627:661	Further structural characterization	627:661	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	33	theme	→	859:859	arg1	2,4					861:863	→ 2,4	859:863	→ 2,4	859:863	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	4	34	from	Man	567:569	arg1	ratio					596:600	a molar ratio	588:600	a molar ratio of 10.6:23.3:5.2:4.9:56	588:624	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	5	35	theme	main	745:748	arg1	chain					750:754	the main chain	741:754	the main chain connection of CWP-0.2	741:776	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	3	36	theme	various	385:391	arg1	methods					393:399	various methods	385:399	various methods	385:399	The chemical structure of the two polysaccharides was characterized by various methods.
35967821	6	37	from	IL-6	1048:1051	arg1	macrophages					1083:1093	RAW 264.7 macrophages	1073:1093	RAW 264.7 macrophages	1073:1093	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	5	38	theme	2,4	861:863	arg1	chain					850:854	the branched chain	837:854	the branched chain of → 2,4	837:863	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	39	theme	chain	750:754	arg1	-α-l-Rhap-					800:809	→ 2,4)-α-l-Rhap-	794:809	→ 2,4)-α-l-Rhap-	794:809	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	5	39	theme	chain	750:754	arg1	connection					756:765	the main chain connection	741:765	the main chain connection of CWP-0.2	741:776	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	0	40	theme	polysaccharides	76:90	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	40	theme	polysaccharides	76:90	arg1	evaluation					44:53	bioactivity evaluation	32:53	bioactivity evaluation	32:53	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	41	theme	water-extractable	58:74	arg1	polysaccharides					76:90	water-extractable polysaccharides	58:90	water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds	58:132	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	42	from	seeds	128:132	arg1	characterization					11:26	Structural characterization	0:26	Structural characterization	0:26	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	42	from	seeds	128:132	arg1	evaluation					44:53	bioactivity evaluation	32:53	bioactivity evaluation	32:53	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	42	from	seeds	128:132	arg1	polysaccharides					76:90	water-extractable polysaccharides	58:90	water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds	58:132	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	5	43	theme	-α-l-Rhap-	865:874	arg1	o-4					880:882	-α-l-Rhap-(1 → o-4	865:882	-α-l-Rhap-(1 → o-4	865:882	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	6	44	from	TNF-α	1054:1058	arg1	macrophages					1083:1093	RAW 264.7 macrophages	1073:1093	RAW 264.7 macrophages	1073:1093	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	6	45	theme	immunoregulatory	935:950	arg1	activity					952:959	antioxidant and immunoregulatory activity	919:959	antioxidant and immunoregulatory activity	919:959	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	4	46	theme	molar	503:507	arg1	ratio					509:513	a molar ratio	501:513	a molar ratio of 44.6:55.4, and CWP-0.2	501:539	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	5	47	dep	indicated	663:671	arg1	-α-d-Galp-					817:826	1 → 3)-α-d-Galp-	811:826	1 → 3)-α-d-Galp-	811:826	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	6	48	theme	stimulating	1018:1028	arg1	production					1030:1039	stimulating production	1018:1039	stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages	1018:1093	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	6	49	theme	NO	1044:1045	arg1	DPPH·					990:994	scavenging DPPH·	979:994	scavenging DPPH·	979:994	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	6	49	theme	NO	1044:1045	arg1	production					1030:1039	stimulating production	1018:1039	stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages	1018:1093	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	6	49	theme	NO	1044:1045	arg1	ABTS·+					1000:1005	ABTS·+	1000:1005	ABTS·+	1000:1005	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	1	50	theme	water-extractable	139:155	arg1	fractions					172:180	Two water-extractable polysaccharide fractions	135:180	Two water-extractable polysaccharide fractions	135:180	Two water-extractable polysaccharide fractions designated as CWP (7.
35967821	6	51	theme	scavenging	979:988	arg1	DPPH·					990:994	scavenging DPPH·	979:994	scavenging DPPH·	979:994	Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS·+ as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages.
35967821	5	52	theme	branched	841:848	arg1	chain					850:854	the branched chain	837:854	the branched chain of → 2,4	837:863	Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fruf-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rhap-(1 → 3)-α-d-Galp-(1 → with the branched chain of → 2,4)-α-l-Rhap-(1 → o-4.
35967821	1	53	theme	polysaccharide	157:170	arg1	fractions					172:180	Two water-extractable polysaccharide fractions	135:180	Two water-extractable polysaccharide fractions	135:180	Two water-extractable polysaccharide fractions designated as CWP (7.
35967821	2	54	dep	×	207:207	arg1	Da					213:214	105 Da	209:214	105 Da	209:214	37 × 105 Da) and CWP-0.2 (1.58 × 104 Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds.
35967821	0	55	theme	chickpeas	97:105	arg1	Cicer					108:112	Cicer	108:112	Cicer	108:112	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	0	55	theme	chickpeas	97:105	arg1	seeds					128:132	chickpeas (Cicer arietinum L.) seeds	97:132	chickpeas (Cicer arietinum L.) seeds	97:132	Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas (Cicer arietinum L.) seeds.
35967821	4	56	theme	Monosaccharide	402:415	arg1	composition					417:427	Monosaccharide composition	402:427	Monosaccharide composition	402:427	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
35967821	2	57	theme	104	237:239	arg1	Da					241:242	1.58 × 104 Da	230:242	1.58 × 104 Da	230:242	37 × 105 Da) and CWP-0.2 (1.58 × 104 Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds.
35967821	2	57	theme	104	237:239	arg1	CWP-0.2					221:227	CWP-0.2	221:227	CWP-0.2	221:227	37 × 105 Da) and CWP-0.2 (1.58 × 104 Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds.
35967821	2	58	theme	×	235:235	arg1	Da					241:242	1.58 × 104 Da	230:242	1.58 × 104 Da	230:242	37 × 105 Da) and CWP-0.2 (1.58 × 104 Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds.
35967821	2	58	theme	×	235:235	arg1	CWP-0.2					221:227	CWP-0.2	221:227	CWP-0.2	221:227	37 × 105 Da) and CWP-0.2 (1.58 × 104 Da) were isolated and purified from chickpea (Cicer arietinum L.) seeds.
35967821	4	59	theme	molar	590:594	arg1	ratio					596:600	a molar ratio	588:600	a molar ratio of 10.6:23.3:5.2:4.9:56	588:624	Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56.
36860492	8	0	theme	gut	1125:1127	arg1	composition					1139:1149	gut microbial composition	1125:1149	gut microbial composition	1125:1149	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	7	1	theme	fatty	905:909	arg1	acids					911:915	short-chain fatty acids	893:915	short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution	893:997	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	6	2	theme	molecular	721:729	arg1	weight					731:736	the molecular weight	717:736	the molecular weight	717:736	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	7	3	from	decrease	951:958	arg1	pH					967:968	the pH	963:968	the pH of the fermentation solution	963:997	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	7	4	theme	gut	841:843	arg1	microbiota					845:854	human gut microbiota	835:854	human gut microbiota	835:854	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	8	5	from	shifts	1111:1116	arg1	composition					1139:1149	gut microbial composition	1125:1149	gut microbial composition	1125:1149	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	8	5	from	shifts	1111:1116	arg1	diversity					1165:1173	community diversity	1155:1173	community diversity	1155:1173	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	5	6	theme	human	436:440	arg1	Methods					367:373	Methods	367:373	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation	367:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	6	theme	human	436:440	arg1	fermentation					448:459	human fecal fermentation	436:459	human fecal fermentation	436:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	9	7	theme	targeted	1275:1282	arg1	promotion					1284:1292	a targeted promotion	1273:1292	a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides,	1273:1381	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	8	8	dep	composition	1139:1149	arg1	the					1121:1123	the	1121:1123	the	1121:1123	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	6	9	from	change	707:712	arg1	weight					731:736	the molecular weight	717:736	the molecular weight	717:736	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	6	9	from	change	707:712	arg1	content					636:642	the content	632:642	the content of the reducing end of the polysaccharide chain	632:690	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	5	10	theme	fecal	442:446	arg1	Methods					367:373	Methods	367:373	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation	367:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	10	theme	fecal	442:446	arg1	fermentation					448:459	human fecal fermentation	436:459	human fecal fermentation	436:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	11	theme	intestinal	529:538	arg1	microflora					540:549	intestinal microflora	529:549	intestinal microflora	529:549	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	12	theme	present	569:575	arg1	study					577:581	the present study	565:581	the present study	565:581	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	9	13	from	increase	1390:1397	arg1	level					1417:1421	the n-butyrate level	1402:1421	the n-butyrate level	1402:1421	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	5	14	theme	LDSPs	498:502	arg1	effects					487:493	the effects	483:493	the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study	483:581	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	9	15	from	promotion	1284:1292	arg1	level					1417:1421	the n-butyrate level	1402:1421	the n-butyrate level	1402:1421	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	8	16	theme	community	1155:1163	arg1	diversity					1165:1173	community diversity	1155:1173	community diversity	1155:1173	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	2	17	theme	Lyophyllum	147:156	arg1	decastes					158:165	Introduction Lyophyllum decastes	134:165	Introduction Lyophyllum decastes (Fr.)	134:171	Introduction Lyophyllum decastes (Fr.)
36860492	3	18	theme	strong	234:239	arg1	properties					252:261	strong biological properties	234:261	strong biological properties	234:261	Singer polysaccharides (LDSPs) have been verified to possess strong biological properties.
36860492	8	19	theme	microbial	1129:1137	arg1	composition					1139:1149	gut microbial composition	1125:1149	gut microbial composition	1125:1149	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	6	20	dep	in	745:746	arg1	vitro					748:752	vitro	748:752	vitro	748:752	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	5	21	from	regulation	551:560	arg1	study					577:581	the present study	565:581	the present study	565:581	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	0	22	from	decastes	43:50	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from Lyophyllum decastes (Fr.)	0:56	Effects of polysaccharides from Lyophyllum decastes (Fr.)
36860492	3	23	theme	biological	241:250	arg1	properties					252:261	strong biological properties	234:261	strong biological properties	234:261	Singer polysaccharides (LDSPs) have been verified to possess strong biological properties.
36860492	7	24	used	utilized	823:830	arg2	LDSPs					799:803	LDSPs	799:803	LDSPs	799:803	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	10	25	dep	Discussion	1424:1433	arg1	suggest					1450:1456	suggest	1450:1456	suggest that LDSPs might be a potential prebiotic to provide a health benefit	1450:1526	Discussion These findings suggest that LDSPs might be a potential prebiotic to provide a health benefit.
36860492	4	26	theme	LDSPs	288:292	arg1	effects					277:283	the effects	273:283	the effects of LDSPs on intestinal microbes and their metabolites	273:337	However, the effects of LDSPs on intestinal microbes and their metabolites have rarely been addressed.
36860492	9	27	theme	n-butyrate	1406:1415	arg1	level					1417:1421	the n-butyrate level	1402:1421	the n-butyrate level	1402:1421	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	9	28	theme	abundance	1301:1309	arg1	increase					1390:1397	an increase	1387:1397	an increase in the n-butyrate level	1387:1421	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	9	28	theme	abundance	1301:1309	arg1	promotion					1284:1292	a targeted promotion	1273:1292	a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides,	1273:1381	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	1	29	theme	gut	68:70	arg1	microbiota					72:81	gut microbiota	68:81	gut microbiota	68:81	Singer on gut microbiota via in vitro-simulated digestion and fermentation.
36860492	6	30	theme	obvious	699:705	arg1	change					707:712	no obvious change	696:712	no obvious change in the molecular weight	696:736	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	4	31	from	effects	277:283	arg1	microbes					308:315	intestinal microbes	297:315	intestinal microbes	297:315	However, the effects of LDSPs on intestinal microbes and their metabolites have rarely been addressed.
36860492	4	31	from	effects	277:283	arg1	metabolites					327:337	their metabolites	321:337	their metabolites	321:337	However, the effects of LDSPs on intestinal microbes and their metabolites have rarely been addressed.
36860492	9	32	theme	butyrogenic	1314:1324	arg1	bacteria					1326:1333	butyrogenic bacteria	1314:1333	butyrogenic bacteria	1314:1333	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	9	32	theme	butyrogenic	1314:1324	arg1	Blautia					1346:1352	Blautia	1346:1352	Blautia	1346:1352	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	9	32	theme	butyrogenic	1314:1324	arg1	Bacteroides					1370:1380	Bacteroides	1370:1380	Bacteroides	1370:1380	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	9	32	theme	butyrogenic	1314:1324	arg1	Roseburia					1355:1363	Roseburia	1355:1363	Roseburia	1355:1363	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	8	33	theme	overall	1044:1050	arg1	structure					1052:1060	the overall structure	1040:1060	the overall structure of LDSPs	1040:1069	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	3	34	theme	Singer	173:178	arg1	polysaccharides					180:194	Singer polysaccharides	173:194	Singer polysaccharides (LDSPs)	173:202	Singer polysaccharides (LDSPs) have been verified to possess strong biological properties.
36860492	3	34	theme	Singer	173:178	arg1	LDSPs					197:201	LDSPs	197:201	LDSPs	197:201	Singer polysaccharides (LDSPs) have been verified to possess strong biological properties.
36860492	6	35	theme	slight	613:618	arg1	increase					620:627	a slight increase	611:627	a slight increase in the content of the reducing end of the polysaccharide chain	611:690	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	6	36	theme	in	745:746	arg1	digestion					754:762	in vitro digestion	745:762	in vitro digestion	745:762	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	8	37	theme	LDSPs	1065:1069	arg1	structure					1052:1060	the overall structure	1040:1060	the overall structure of LDSPs	1040:1069	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	7	38	theme	human	835:839	arg1	microbiota					845:854	human gut microbiota	835:854	human gut microbiota	835:854	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	5	39	used	used	466:469	arg2	digestion					422:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	39	used	used	466:469	arg2	Methods					367:373	Methods	367:373	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation	367:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	39	used	used	466:469	arg2	fermentation					448:459	human fecal fermentation	436:459	human fecal fermentation	436:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	2	40	dep	decastes	158:165	arg1	Fr					168:169	Fr	168:169	Fr.	168:170	Introduction Lyophyllum decastes (Fr.)
36860492	6	41	dep	Results	584:590	arg1	showed					604:609	showed	604:609	showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion	604:762	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	5	42	dep	vitro-simulated	382:396	arg1	in					379:380	in	379:380	in	379:380	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	6	43	theme	chain	686:690	arg1	end					660:662	the reducing end	647:662	the reducing end of the polysaccharide chain	647:690	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	8	44	theme	16S	1075:1077	arg1	rRNA					1079:1082	16S rRNA	1075:1082	16S rRNA analysis	1075:1091	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	7	45	dep	significant	928:938	arg1	p < 0.05					941:948	p < 0.05	941:948	p < 0.05	941:948	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	5	46	from	effects	487:493	arg1	regulation					551:560	non-digestibility and intestinal microflora regulation	507:560	non-digestibility and intestinal microflora regulation in the present study	507:581	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	6	47	theme	polysaccharide	671:684	arg1	chain					686:690	the polysaccharide chain	667:690	the polysaccharide chain	667:690	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	9	48	theme	bacteria	1326:1333	arg1	abundance					1301:1309	the abundance	1297:1309	the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides,	1297:1381	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	0	49	theme	Lyophyllum	32:41	arg1	decastes					43:50	Lyophyllum decastes	32:50	Lyophyllum decastes (Fr.)	32:56	Effects of polysaccharides from Lyophyllum decastes (Fr.)
36860492	7	50	theme	short-chain	893:903	arg1	acids					911:915	short-chain fatty acids	893:915	short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution	893:997	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	8	51	theme	LDSPs-treated	1182:1194	arg1	cultures					1196:1203	the LDSPs-treated cultures	1178:1203	the LDSPs-treated cultures	1178:1203	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	8	52	theme	cultures	1196:1203	arg1	composition					1139:1149	gut microbial composition	1125:1149	gut microbial composition	1125:1149	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	8	52	theme	cultures	1196:1203	arg1	diversity					1165:1173	community diversity	1155:1173	community diversity	1155:1173	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	6	53	from	increase	620:627	arg1	content					636:642	the content	632:642	the content of the reducing end of the polysaccharide chain	632:690	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	6	53	from	increase	620:627	arg1	weight					731:736	the molecular weight	717:736	the molecular weight	717:736	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	0	54	dep	decastes	43:50	arg1	Fr					53:54	Fr	53:54	Fr.	53:55	Effects of polysaccharides from Lyophyllum decastes (Fr.)
36860492	2	55	theme	Introduction	134:145	arg1	decastes					158:165	Introduction Lyophyllum decastes	134:165	Introduction Lyophyllum decastes (Fr.)	134:171	Introduction Lyophyllum decastes (Fr.)
36860492	1	56	theme	vitro-simulated	90:104	arg1	digestion					106:114	vitro-simulated digestion	90:114	vitro-simulated digestion	90:114	Singer on gut microbiota via in vitro-simulated digestion and fermentation.
36860492	7	57	theme	24 h	771:774	arg1	fermentation					785:796	24 h in vitro fermentation	771:796	24 h in vitro fermentation	771:796	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	8	58	theme	rRNA	1079:1082	arg1	analysis					1084:1091	16S rRNA analysis	1075:1091	16S rRNA analysis	1075:1091	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	7	59	theme	significant	928:938	arg1	decrease					951:958	significant (p < 0.05) decrease	928:958	significant (p < 0.05) decrease in the pH of the fermentation solution	928:997	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	7	60	theme	fermentation	977:988	arg1	solution					990:997	the fermentation solution	973:997	the fermentation solution	973:997	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	3	61	contain	possess	226:232	arg1	polysaccharides					180:194	Singer polysaccharides	173:194	Singer polysaccharides (LDSPs)	173:202	Singer polysaccharides (LDSPs) have been verified to possess strong biological properties.
36860492	3	61	contain	possess	226:232	arg1	LDSPs					197:201	LDSPs	197:201	LDSPs	197:201	Singer polysaccharides (LDSPs) have been verified to possess strong biological properties.
36860492	3	61	contain	possess	226:232	arg2	properties					252:261	strong biological properties	234:261	strong biological properties	234:261	Singer polysaccharides (LDSPs) have been verified to possess strong biological properties.
36860492	7	62	theme	in	776:777	arg1	fermentation					785:796	24 h in vitro fermentation	771:796	24 h in vitro fermentation	771:796	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	8	63	theme	distinct	1102:1109	arg1	shifts					1111:1116	distinct shifts	1102:1116	distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures	1102:1203	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	5	64	dep	Methods	367:373	arg1	digestion					422:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	64	dep	Methods	367:373	arg1	Methods					367:373	Methods	367:373	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation	367:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	64	dep	Methods	367:373	arg1	fermentation					448:459	human fecal fermentation	436:459	human fecal fermentation	436:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	7	65	theme	solution	990:997	arg1	pH					967:968	the pH	963:968	the pH of the fermentation solution	963:997	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	7	66	dep	in	776:777	arg1	vitro					779:783	vitro	779:783	vitro	779:783	After 24 h in vitro fermentation, LDSPs were degraded and utilized by human gut microbiota, and LDSPs could be transformed into short-chain fatty acids leading to significant (p < 0.05) decrease in the pH of the fermentation solution.
36860492	4	67	theme	intestinal	297:306	arg1	microbes					308:315	intestinal microbes	297:315	intestinal microbes	297:315	However, the effects of LDSPs on intestinal microbes and their metabolites have rarely been addressed.
36860492	10	68	theme	potential	1480:1488	arg1	prebiotic					1490:1498	a potential prebiotic	1478:1498	a potential prebiotic to provide a health benefit	1478:1526	Discussion These findings suggest that LDSPs might be a potential prebiotic to provide a health benefit.
36860492	10	68	theme	potential	1480:1488	arg1	LDSPs					1463:1467	LDSPs	1463:1467	LDSPs	1463:1467	Discussion These findings suggest that LDSPs might be a potential prebiotic to provide a health benefit.
36860492	6	69	theme	end	660:662	arg1	content					636:642	the content	632:642	the content of the reducing end of the polysaccharide chain	632:690	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	5	70	theme	non-digestibility	507:523	arg1	regulation					551:560	non-digestibility and intestinal microflora regulation	507:560	non-digestibility and intestinal microflora regulation in the present study	507:581	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	9	71	theme	LDSPs	1252:1256	arg1	group					1258:1262	the LDSPs group	1248:1262	the LDSPs group	1248:1262	Notably, the LDSPs group directed a targeted promotion of the abundance of butyrogenic bacteria, including Blautia, Roseburia, and Bacteroides, and an increase in the n-butyrate level.
36860492	5	72	theme	microflora	540:549	arg1	regulation					551:560	non-digestibility and intestinal microflora regulation	507:560	non-digestibility and intestinal microflora regulation in the present study	507:581	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	6	73	theme	reducing	651:658	arg1	end					660:662	the reducing end	647:662	the reducing end of the polysaccharide chain	647:690	Results The results showed a slight increase in the content of the reducing end of the polysaccharide chain and no obvious change in the molecular weight during in vitro digestion.
36860492	5	74	theme	vitro-simulated	382:396	arg1	digestion					422:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	74	theme	vitro-simulated	382:396	arg1	Methods					367:373	Methods	367:373	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation	367:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	10	75	theme	health	1513:1518	arg1	benefit					1520:1526	a health benefit	1511:1526	a health benefit	1511:1526	Discussion These findings suggest that LDSPs might be a potential prebiotic to provide a health benefit.
36860492	0	76	theme	polysaccharides	11:25	arg1	Effects					0:6	Effects	0:6	Effects of polysaccharides from Lyophyllum decastes (Fr.)	0:56	Effects of polysaccharides from Lyophyllum decastes (Fr.)
36860492	8	77	theme	control	1224:1230	arg1	group					1232:1236	the control group	1220:1236	the control group	1220:1236	The digestion did not remarkably affect the overall structure of LDSPs and 16S rRNA analysis revealed distinct shifts in the gut microbial composition and community diversity of the LDSPs-treated cultures, compared with the control group.
36860492	1	78	from	Singer	58:63	arg1	microbiota					72:81	gut microbiota	68:81	gut microbiota	68:81	Singer on gut microbiota via in vitro-simulated digestion and fermentation.
36860492	1	78	from	Singer	58:63	arg1	fermentation					120:131	fermentation	120:131	fermentation	120:131	Singer on gut microbiota via in vitro-simulated digestion and fermentation.
36860492	1	78	from	Singer	58:63	arg1	digestion					106:114	vitro-simulated digestion	90:114	vitro-simulated digestion	90:114	Singer on gut microbiota via in vitro-simulated digestion and fermentation.
36860492	5	79	theme	saliva-gastrointestinal	398:420	arg1	digestion					422:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	The in vitro-simulated saliva-gastrointestinal digestion	375:430	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36860492	5	79	theme	saliva-gastrointestinal	398:420	arg1	Methods					367:373	Methods	367:373	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation	367:459	Methods The in vitro-simulated saliva-gastrointestinal digestion and human fecal fermentation were used to evaluate the effects of LDSPs on non-digestibility and intestinal microflora regulation in the present study.
36506785	0	0	theme	different	81:89	arg1	methods					91:97	different methods	81:97	different methods	81:97	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	0	1	from	study	13:17	arg1	polysaccharides					22:36	polysaccharides	22:36	polysaccharides extracted from Rosa sterilis S.D.Shi using different methods	22:97	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	1	2	theme	polysaccharides	255:269	arg1	characterizations					212:228	the structural and in vitro fermentation characterizations	171:228	the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM)	171:344	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	8	3	theme	RSPs	1380:1383	arg1	structure					1344:1352	structure	1344:1352	structure	1344:1352	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	8	3	theme	RSPs	1380:1383	arg1	function					1368:1375	probiotic function	1358:1375	probiotic function	1358:1375	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	4	4	from	rich	662:665	arg1	RG-I					670:673	RG-I	670:673	RG-I	670:673	EM-RSP was rich in RG-I and its size of average side chain were the largest.
36506785	0	5	dep	in	115:116	arg1	vitro					118:122	vitro	118:122	vitro	118:122	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	7	6	theme	special	1234:1240	arg1	pathways					1252:1259	the most special metabolic pathways	1225:1259	the most special metabolic pathways	1225:1259	Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways.
36506785	2	7	theme	probiotic	514:522	arg1	effects					524:530	different probiotic effects	504:530	different probiotic effects	504:530	The results indicated that extraction methods exhibited significant effects on the structure of RSPs, thus resulting in different probiotic effects.
36506785	7	8	theme	metabolic	1242:1250	arg1	pathways					1252:1259	the most special metabolic pathways	1225:1259	the most special metabolic pathways	1225:1259	Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways.
36506785	2	9	theme	different	504:512	arg1	effects					524:530	different probiotic effects	504:530	different probiotic effects	504:530	The results indicated that extraction methods exhibited significant effects on the structure of RSPs, thus resulting in different probiotic effects.
36506785	6	10	theme	gut	1141:1143	arg1	microbiota					1145:1154	gut microbiota	1141:1154	gut microbiota	1141:1154	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	5	11	theme	molecular	816:824	arg1	weights					826:832	molecular weights	816:832	molecular weights	816:832	Moreover, HW-RSP and AK-RSP exhibited the smallest (57.55 kDa) and largest (922.20 kDa) molecular weights, respectively.
36506785	8	12	theme	functional	1437:1446	arg1	products					1448:1455	functional products	1437:1455	functional products of Rosa sterilis S.D.Shi	1437:1480	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	2	13	theme	significant	440:450	arg1	effects					452:458	significant effects	440:458	significant effects	440:458	The results indicated that extraction methods exhibited significant effects on the structure of RSPs, thus resulting in different probiotic effects.
36506785	8	14	theme	theoretical	1396:1406	arg1	basis					1408:1412	theoretical basis	1396:1412	theoretical basis for the development of functional products of Rosa sterilis S.D.Shi	1396:1480	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	1	15	theme	first	372:376	arg1	time					378:381	the first time	368:381	the first time	368:381	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	5	16	dep	smallest	770:777	arg1	weights					826:832	molecular weights	816:832	molecular weights	816:832	Moreover, HW-RSP and AK-RSP exhibited the smallest (57.55 kDa) and largest (922.20 kDa) molecular weights, respectively.
36506785	6	17	theme	bacteria	926:933	arg1	production					871:880	the production	867:880	the production of total SCFAs	867:895	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	6	17	theme	bacteria	926:933	arg1	growth					905:910	the growth	901:910	the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees	901:1022	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	0	18	theme	Structural	100:109	arg1	characterizations					137:153	Structural and in vitro fermentation characterizations	100:153	Structural and in vitro fermentation characterizations	100:153	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	4	19	theme	chain	704:708	arg1	largest					719:725	largest	719:725	largest	719:725	EM-RSP was rich in RG-I and its size of average side chain were the largest.
36506785	4	19	theme	chain	704:708	arg1	size					683:686	its size	679:686	its size of average side chain	679:708	EM-RSP was rich in RG-I and its size of average side chain were the largest.
36506785	7	20	contain	had	1221:1223	arg2	pathways					1252:1259	the most special metabolic pathways	1225:1259	the most special metabolic pathways	1225:1259	Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways.
36506785	7	20	contain	had	1221:1223	arg1	EM-RSP					1214:1219	EM-RSP	1214:1219	EM-RSP	1214:1219	Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways.
36506785	6	21	theme	beneficial	915:924	arg1	bacteria					926:933	beneficial bacteria	915:933	beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees	915:1022	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	0	22	theme	comparison	2:11	arg1	study					13:17	A comparison study	0:17	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods	0:97	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	8	23	theme	new	1297:1299	arg1	insights					1301:1308	new insights	1297:1308	new insights into the relationship between the structure and probiotic function of RSPs	1297:1383	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	1	24	theme	structural	175:184	arg1	characterizations					212:228	the structural and in vitro fermentation characterizations	171:228	the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM)	171:344	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	7	25	theme	most	1229:1232	arg1	pathways					1252:1259	the most special metabolic pathways	1225:1259	the most special metabolic pathways	1225:1259	Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways.
36506785	6	26	theme	pathogenic	1053:1062	arg1	bacteria					1064:1071	pathogenic bacteria	1053:1071	pathogenic bacteria such as Escherichia-shigella	1053:1100	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	6	26	theme	pathogenic	1053:1062	arg1	Escherichia-shigella					1081:1100	Escherichia-shigella	1081:1100	Escherichia-shigella	1081:1100	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	2	27	theme	RSPs	480:483	arg1	structure					467:475	the structure	463:475	the structure of RSPs	463:483	The results indicated that extraction methods exhibited significant effects on the structure of RSPs, thus resulting in different probiotic effects.
36506785	6	28	theme	SCFAs	891:895	arg1	production					871:880	the production	867:880	the production of total SCFAs	867:895	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	6	28	theme	SCFAs	891:895	arg1	growth					905:910	the growth	901:910	the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees	901:1022	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	1	29	theme	in	190:191	arg1	characterizations					212:228	the structural and in vitro fermentation characterizations	171:228	the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM)	171:344	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	8	30	theme	products	1448:1455	arg1	development					1422:1432	the development	1418:1432	the development of functional products of Rosa sterilis S.D.Shi	1418:1480	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	0	31	theme	in	115:116	arg1	characterizations					137:153	Structural and in vitro fermentation characterizations	100:153	Structural and in vitro fermentation characterizations	100:153	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	7	32	theme	prediction	1183:1192	arg1	results					1194:1200	the function prediction results	1170:1200	the function prediction results	1170:1200	Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways.
36506785	1	33	theme	hot	291:293	arg1	HW					302:303	HW	302:303	HW	302:303	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	1	33	theme	hot	291:293	arg1	water					295:299	hot water	291:299	hot water (HW)	291:304	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	3	34	theme	Gal	572:574	arg1	contents					560:567	high contents	555:567	high contents of Gal, Glc and GalA	555:588	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	1	35	theme	fermentation	199:210	arg1	characterizations					212:228	the structural and in vitro fermentation characterizations	171:228	the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM)	171:344	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	8	36	dep	structure	1344:1352	arg1	the					1340:1342	the	1340:1342	the	1340:1342	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	0	37	theme	fermentation	124:135	arg1	characterizations					137:153	Structural and in vitro fermentation characterizations	100:153	Structural and in vitro fermentation characterizations	100:153	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	3	38	theme	high	555:558	arg1	contents					560:567	high contents	555:567	high contents of Gal, Glc and GalA	555:588	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	3	39	contain	had	551:553	arg1	AA-RSP					544:549	AA-RSP	544:549	AA-RSP	544:549	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	3	39	contain	had	551:553	arg2	contents					560:567	high contents	555:567	high contents of Gal, Glc and GalA	555:588	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	3	39	contain	had	551:553	arg1	HW-RSP					533:538	HW-RSP	533:538	HW-RSP	533:538	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	6	40	theme	total	885:889	arg1	SCFAs					891:895	total SCFAs	885:895	total SCFAs	885:895	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	4	41	theme	side	699:702	arg1	chain					704:708	average side chain	691:708	average side chain	691:708	EM-RSP was rich in RG-I and its size of average side chain were the largest.
36506785	8	42	theme	S.D.Shi	1474:1480	arg1	products					1448:1455	functional products	1437:1455	functional products of Rosa sterilis S.D.Shi	1437:1480	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	8	43	theme	probiotic	1358:1366	arg1	function					1368:1375	probiotic function	1358:1375	probiotic function	1358:1375	Collectively, our findings provide new insights into the relationship between the structure and probiotic function of RSPs, and offer theoretical basis for the development of functional products of Rosa sterilis S.D.Shi.
36506785	4	44	theme	average	691:697	arg1	chain					704:708	average side chain	691:708	average side chain	691:708	EM-RSP was rich in RG-I and its size of average side chain were the largest.
36506785	6	45	theme	microbiota	1145:1154	arg1	composition					1126:1136	the composition	1122:1136	the composition of gut microbiota	1122:1154	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	3	46	theme	GalA	585:588	arg1	contents					560:567	high contents	555:567	high contents of Gal, Glc and GalA	555:588	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	6	47	dep	Faecalibacterium	970:985	arg1	Bacteroides					957:967	Bacteroides	957:967	Bacteroides	957:967	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	6	48	theme	varying	1008:1014	arg1	degrees					1016:1022	varying degrees	1008:1022	varying degrees	1008:1022	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	6	49	theme	bacteria	1064:1071	arg1	growth					1043:1048	the growth	1039:1048	the growth of pathogenic bacteria such as Escherichia-shigella	1039:1100	All RSPs promoted the production of total SCFAs and the growth of beneficial bacteria like Bifidobacterium, Bacteroides, Faecalibacterium and Paraclostrium to varying degrees, but inhibited the growth of pathogenic bacteria such as Escherichia-shigella, thereby regulating the composition of gut microbiota.
36506785	3	50	contain	contained	622:630	arg2	Ara					632:634	Ara	632:634	Ara	632:634	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	3	50	contain	contained	622:630	arg1	AK-RSP					597:602	AK-RSP	597:602	AK-RSP	597:602	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	3	50	contain	contained	622:630	arg2	GalA					645:648	GalA	645:648	GalA	645:648	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	3	50	contain	contained	622:630	arg2	Gal					637:639	Gal	637:639	Gal	637:639	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	3	50	contain	contained	622:630	arg1	EM-RSP					608:613	EM-RSP	608:613	EM-RSP	608:613	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	1	51	theme	Rosa	233:236	arg1	RSP					272:274	RSP	272:274	RSP	272:274	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	1	51	theme	Rosa	233:236	arg1	polysaccharides					255:269	Rosa sterilis S.D.Shi polysaccharides	233:269	Rosa sterilis S.D.Shi polysaccharides (RSP)	233:275	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	3	52	theme	Glc	577:579	arg1	contents					560:567	high contents	555:567	high contents of Gal, Glc and GalA	555:588	HW-RSP and AA-RSP had high contents of Gal, Glc and GalA, while AK-RSP and EM-RSP mainly contained Ara, Gal and GalA.
36506785	1	53	dep	in	190:191	arg1	vitro					193:197	vitro	193:197	vitro	193:197	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	7	54	theme	function	1174:1181	arg1	results					1194:1200	the function prediction results	1170:1200	the function prediction results	1170:1200	Furthermore, the function prediction results showed that EM-RSP had the most special metabolic pathways.
36506785	0	55	dep	characterizations	137:153	arg1	study					13:17	A comparison study	0:17	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods	0:97	A comparison study on polysaccharides extracted from Rosa sterilis S.D.Shi using different methods: Structural and in vitro fermentation characterizations.
36506785	1	56	theme	sterilis	238:245	arg1	RSP					272:274	RSP	272:274	RSP	272:274	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	1	56	theme	sterilis	238:245	arg1	polysaccharides					255:269	Rosa sterilis S.D.Shi polysaccharides	233:269	Rosa sterilis S.D.Shi polysaccharides (RSP)	233:275	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	2	57	theme	extraction	411:420	arg1	methods					422:428	extraction methods	411:428	extraction methods	411:428	The results indicated that extraction methods exhibited significant effects on the structure of RSPs, thus resulting in different probiotic effects.
36506785	4	58	from	RG-I	670:673	arg1	rich					662:665	rich	662:665	rich	662:665	EM-RSP was rich in RG-I and its size of average side chain were the largest.
36506785	1	59	theme	S.D.Shi	247:253	arg1	RSP					272:274	RSP	272:274	RSP	272:274	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
36506785	1	59	theme	S.D.Shi	247:253	arg1	polysaccharides					255:269	Rosa sterilis S.D.Shi polysaccharides	233:269	Rosa sterilis S.D.Shi polysaccharides (RSP)	233:275	In this study, the structural and in vitro fermentation characterizations of Rosa sterilis S.D.Shi polysaccharides (RSP), extracted by hot water (HW), acid (AA), alkali (AK) and enzyme (EM) were investigated for the first time.
35359717	9	0	theme	HO-1	1201:1204	arg1	abundances					1168:1177	the protein abundances	1156:1177	the protein abundances of p-Nrf2, Keap1, and HO-1 in mice	1156:1212	Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice.
35359717	4	1	theme	present	465:471	arg1	study					473:477	the present study	461:477	the present study	461:477	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	9	2	theme	protein	1160:1166	arg1	abundances					1168:1177	the protein abundances	1156:1177	the protein abundances of p-Nrf2, Keap1, and HO-1 in mice	1156:1212	Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice.
35359717	9	3	theme	SSPs	1142:1145	arg1	administration					1124:1137	the administration	1120:1137	the administration of SSPs	1120:1145	Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice.
35359717	11	4	theme	intestinal	1496:1505	arg1	microbiota					1507:1516	intestinal microbiota	1496:1516	intestinal microbiota	1496:1516	To summarize, SSPs benefit intestinal health in C57BL/6J mice via a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.
35359717	10	5	theme	16S	1230:1232	arg1	rDNA					1234:1237	16S rDNA	1230:1237	16S rDNA	1230:1237	The results of 16S rDNA demonstrated that the microbial community and composition were altered by SSPs administration.
35359717	1	6	theme	edible	163:168	arg1	fungus					170:175	an edible fungus	160:175	an edible fungus	160:175	Scorias spongiosa, as an edible fungus, has multiple health benefits.
35359717	0	7	theme	Intestinal	107:116	arg1	Microbiota					118:127	Intestinal Microbiota	107:127	Intestinal Microbiota in Mice	107:135	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	4	8	dep	groups	530:535	arg1	group					550:554	(1) Control group	538:554	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	8	dep	groups	530:535	arg1	CON					557:559	CON	557:559	CON	557:559	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	7	9	theme	pro-inflammatory	867:882	arg1	cytokines					884:892	the serum pro-inflammatory cytokines	857:892	the serum pro-inflammatory cytokines	857:892	Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines.
35359717	11	10	dep	summarize	1337:1345	arg1	To					1334:1335	To	1334:1335	To	1334:1335	To summarize, SSPs benefit intestinal health in C57BL/6J mice via a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.
35359717	10	11	theme	SSPs	1313:1316	arg1	administration					1318:1331	SSPs administration	1313:1331	SSPs administration	1313:1331	The results of 16S rDNA demonstrated that the microbial community and composition were altered by SSPs administration.
35359717	11	12	theme	intestinal	1361:1370	arg1	health					1372:1377	intestinal health	1361:1377	intestinal health in C57BL/6J mice	1361:1394	To summarize, SSPs benefit intestinal health in C57BL/6J mice via a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.
35359717	9	13	from	abundances	1168:1177	arg1	mice					1209:1212	mice	1209:1212	mice	1209:1212	Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice.
35359717	4	14	theme	Low	567:569	arg1	LSSP					588:591	LSSP	588:591	LSSP	588:591	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	14	theme	Low	567:569	arg1	group					581:585	(2) Low dose SSPs group	563:585	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	8	15	theme	increased	1032:1040	arg1	contents					1042:1049	the increased contents	1028:1049	the increased contents of T-AOC, GSH-Px, and the decreased content of MDA	1028:1100	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	4	16	dep	group	581:585	arg1	2					564:564	2	564:564	2	564:564	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	8	17	theme	content	1087:1093	arg1	contents					1042:1049	the increased contents	1028:1049	the increased contents of T-AOC, GSH-Px, and the decreased content of MDA	1028:1100	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	0	18	theme	spongiosa	8:16	arg1	Polysaccharides					18:32	Scorias spongiosa Polysaccharides	0:32	Scorias spongiosa Polysaccharides	0:32	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	9	19	theme	p-Nrf2	1182:1187	arg1	abundances					1168:1177	the protein abundances	1156:1177	the protein abundances of p-Nrf2, Keap1, and HO-1 in mice	1156:1212	Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice.
35359717	10	20	theme	microbial	1261:1269	arg1	community					1271:1279	the microbial community	1257:1279	the microbial community	1257:1279	The results of 16S rDNA demonstrated that the microbial community and composition were altered by SSPs administration.
35359717	4	21	theme	dose	604:607	arg1	HSSP					621:624	HSSP	621:624	HSSP	621:624	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	21	theme	dose	604:607	arg1	group					614:618	(3) High dose SSPs group	595:618	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	2	22	from	effects	221:227	arg1	health					259:264	intestinal health	248:264	intestinal health	248:264	However, the effects of S. spongiosa on intestinal health are rarely explored.
35359717	4	23	theme	SSPs	576:579	arg1	LSSP					588:591	LSSP	588:591	LSSP	588:591	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	23	theme	SSPs	576:579	arg1	group					581:585	(2) Low dose SSPs group	563:585	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	0	24	theme	Scorias	0:6	arg1	Polysaccharides					18:32	Scorias spongiosa Polysaccharides	0:32	Scorias spongiosa Polysaccharides	0:32	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	4	25	theme	High	599:602	arg1	HSSP					621:624	HSSP	621:624	HSSP	621:624	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	25	theme	High	599:602	arg1	group					614:618	(3) High dose SSPs group	595:618	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	9	26	theme	Keap1	1190:1194	arg1	abundances					1168:1177	the protein abundances	1156:1177	the protein abundances of p-Nrf2, Keap1, and HO-1 in mice	1156:1212	Mechanistically, the administration of SSPs enhanced the protein abundances of p-Nrf2, Keap1, and HO-1 in mice.
35359717	1	27	theme	multiple	182:189	arg1	benefits					198:205	multiple health benefits	182:205	multiple health benefits	182:205	Scorias spongiosa, as an edible fungus, has multiple health benefits.
35359717	3	28	theme	S.	343:344	arg1	SSPs					373:376	S. spongiosa polysaccharides (SSPs)	343:377	S. spongiosa polysaccharides (SSPs)	343:377	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	5	29	theme	jejunum	661:667	arg1	samples					679:685	the jejunum and serum samples	657:685	the jejunum and serum samples	657:685	After 14-day administration, the jejunum and serum samples were collected for detection.
35359717	10	30	theme	rDNA	1234:1237	arg1	results					1219:1225	The results	1215:1225	The results of 16S rDNA	1215:1237	The results of 16S rDNA demonstrated that the microbial community and composition were altered by SSPs administration.
35359717	11	31	theme	C57BL/6J	1382:1389	arg1	mice					1391:1394	C57BL/6J mice	1382:1394	C57BL/6J mice	1382:1394	To summarize, SSPs benefit intestinal health in C57BL/6J mice via a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.
35359717	1	32	theme	health	191:196	arg1	benefits					198:205	multiple health benefits	182:205	multiple health benefits	182:205	Scorias spongiosa, as an edible fungus, has multiple health benefits.
35359717	3	33	theme	spongiosa	346:354	arg1	SSPs					373:376	S. spongiosa polysaccharides (SSPs)	343:377	S. spongiosa polysaccharides (SSPs)	343:377	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	0	34	from	Effect	97:102	arg1	Microbiota					118:127	Intestinal Microbiota	107:127	Intestinal Microbiota in Mice	107:135	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	4	35	theme	SSPs	609:612	arg1	HSSP					621:624	HSSP	621:624	HSSP	621:624	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	35	theme	SSPs	609:612	arg1	group					614:618	(3) High dose SSPs group	595:618	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	3	36	theme	C57BL/6J	443:450	arg1	mice					452:455	C57BL/6J mice	443:455	C57BL/6J mice	443:455	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	0	37	theme	Antioxidant	46:56	arg1	Capacity					80:87	the Antioxidant and Anti-Inflammatory Capacity	42:87	the Antioxidant and Anti-Inflammatory Capacity	42:87	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	7	38	theme	anti-inflammatory	911:927	arg1	cytokines					929:937	the anti-inflammatory cytokines	907:937	elevated the anti-inflammatory cytokines	898:937	Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines.
35359717	1	39	contain	has	178:180	arg2	benefits					198:205	multiple health benefits	182:205	multiple health benefits	182:205	Scorias spongiosa, as an edible fungus, has multiple health benefits.
35359717	1	39	contain	has	178:180	arg1	spongiosa					146:154	Scorias spongiosa	138:154	Scorias spongiosa	138:154	Scorias spongiosa, as an edible fungus, has multiple health benefits.
35359717	4	40	theme	dose	571:574	arg1	LSSP					588:591	LSSP	588:591	LSSP	588:591	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	40	theme	dose	571:574	arg1	group					581:585	(2) Low dose SSPs group	563:585	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	3	41	theme	intestinal	418:427	arg1	microflora					429:438	intestinal microflora	418:438	intestinal microflora in C57BL/6J mice	418:455	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	5	42	theme	14-day	634:639	arg1	administration					641:654	14-day administration	634:654	14-day administration	634:654	After 14-day administration, the jejunum and serum samples were collected for detection.
35359717	6	43	theme	mice	792:795	arg1	performance					777:787	the growth performance	766:787	the growth performance of mice	766:795	The results showed that SSPs exert no effects on the growth performance of mice regardless of doses.
35359717	8	44	theme	antioxidant	954:964	arg1	capacity					966:973	the antioxidant capacity	950:973	the antioxidant capacity	950:973	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	3	45	from	influences	329:338	arg1	microflora					429:438	intestinal microflora	418:438	intestinal microflora in C57BL/6J mice	418:455	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	3	45	from	influences	329:338	arg1	anti-inflammatory					395:411	anti-inflammatory	395:411	anti-inflammatory	395:411	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	3	45	from	influences	329:338	arg1	antioxidant					382:392	antioxidant	382:392	antioxidant	382:392	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	8	46	theme	GSH-Px	1061:1066	arg1	contents					1042:1049	the increased contents	1028:1049	the increased contents of T-AOC, GSH-Px, and the decreased content of MDA	1028:1100	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	7	47	theme	serum	861:865	arg1	cytokines					884:892	the serum pro-inflammatory cytokines	857:892	the serum pro-inflammatory cytokines	857:892	Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines.
35359717	8	48	theme	T-AOC	1054:1058	arg1	contents					1042:1049	the increased contents	1028:1049	the increased contents of T-AOC, GSH-Px, and the decreased content of MDA	1028:1100	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	3	49	theme	SSPs	373:376	arg1	influences					329:338	the influences	325:338	the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice	325:455	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	4	50	theme	male	483:486	arg1	mice					488:491	18 male mice	480:491	18 male mice	480:491	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	7	51	theme	elevated	898:905	arg1	cytokines					929:937	the anti-inflammatory cytokines	907:937	elevated the anti-inflammatory cytokines	898:937	Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines.
35359717	0	52	from	Capacity	80:87	arg1	Microbiota					118:127	Intestinal Microbiota	107:127	Intestinal Microbiota in Mice	107:135	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	8	53	theme	MDA	1098:1100	arg1	content					1087:1093	the decreased content	1073:1093	the decreased content of MDA	1073:1100	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	8	53	theme	MDA	1098:1100	arg1	GSH-Px					1061:1066	GSH-Px	1061:1066	GSH-Px	1061:1066	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	8	53	theme	MDA	1098:1100	arg1	T-AOC					1054:1058	T-AOC	1054:1058	T-AOC	1054:1058	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	3	54	from	microflora	429:438	arg1	mice					452:455	C57BL/6J mice	443:455	C57BL/6J mice	443:455	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
35359717	4	55	dep	group	614:618	arg1	3					596:596	3	596:596	3	596:596	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	6	56	theme	growth	770:775	arg1	performance					777:787	the growth performance	766:787	the growth performance of mice	766:795	The results showed that SSPs exert no effects on the growth performance of mice regardless of doses.
35359717	8	57	theme	SSPs	991:994	arg1	administration					996:1009	SSPs administration	991:1009	SSPs administration	991:1009	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	0	58	from	Microbiota	118:127	arg1	Mice					132:135	Mice	132:135	Mice	132:135	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	2	59	theme	intestinal	248:257	arg1	health					259:264	intestinal health	248:264	intestinal health	248:264	However, the effects of S. spongiosa on intestinal health are rarely explored.
35359717	1	60	theme	Scorias	138:144	arg1	spongiosa					146:154	Scorias spongiosa	138:154	Scorias spongiosa	138:154	Scorias spongiosa, as an edible fungus, has multiple health benefits.
35359717	4	61	dep	group	550:554	arg1	LSSP					588:591	LSSP	588:591	LSSP	588:591	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	61	dep	group	550:554	arg1	HSSP					621:624	HSSP	621:624	HSSP	621:624	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	61	dep	group	550:554	arg1	group					581:585	(2) Low dose SSPs group	563:585	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	61	dep	group	550:554	arg1	group					614:618	(3) High dose SSPs group	595:618	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	5	62	theme	serum	673:677	arg1	samples					679:685	the jejunum and serum samples	657:685	the jejunum and serum samples	657:685	After 14-day administration, the jejunum and serum samples were collected for detection.
35359717	7	63	theme	SSPs	829:832	arg1	Meanwhile					818:826	Meanwhile	818:826	Meanwhile	818:826	Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines.
35359717	7	63	theme	SSPs	829:832	arg1	administration					834:847	SSPs administration	829:847	SSPs administration	829:847	Meanwhile, SSPs administration reduced the serum pro-inflammatory cytokines and elevated the anti-inflammatory cytokines.
35359717	8	64	theme	decreased	1077:1085	arg1	content					1087:1093	the decreased content	1073:1093	the decreased content of MDA	1073:1100	Moreover, the antioxidant capacity was elevated by SSPs administration, as evidenced by the increased contents of T-AOC, GSH-Px, and the decreased content of MDA.
35359717	4	65	theme	Control	542:548	arg1	group					550:554	(1) Control group	538:554	three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP)	524:625	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	4	65	theme	Control	542:548	arg1	CON					557:559	CON	557:559	CON	557:559	In the present study, 18 male mice were randomly distributed into three groups: (1) Control group (CON); (2) Low dose SSPs group (LSSP); (3) High dose SSPs group (HSSP).
35359717	6	66	from	effects	755:761	arg1	performance					777:787	the growth performance	766:787	the growth performance of mice	766:795	The results showed that SSPs exert no effects on the growth performance of mice regardless of doses.
35359717	2	67	theme	spongiosa	235:243	arg1	effects					221:227	the effects	217:227	the effects of S. spongiosa on intestinal health	217:264	However, the effects of S. spongiosa on intestinal health are rarely explored.
35359717	11	68	from	health	1372:1377	arg1	mice					1391:1394	C57BL/6J mice	1382:1394	C57BL/6J mice	1382:1394	To summarize, SSPs benefit intestinal health in C57BL/6J mice via a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.
35359717	0	69	theme	Anti-Inflammatory	62:78	arg1	Capacity					80:87	the Antioxidant and Anti-Inflammatory Capacity	42:87	the Antioxidant and Anti-Inflammatory Capacity	42:87	Scorias spongiosa Polysaccharides Promote the Antioxidant and Anti-Inflammatory Capacity and Its Effect on Intestinal Microbiota in Mice.
35359717	2	70	theme	S.	232:233	arg1	spongiosa					235:243	S. spongiosa	232:243	S. spongiosa	232:243	However, the effects of S. spongiosa on intestinal health are rarely explored.
35359717	11	71	theme	anti-inflammatory	1452:1468	arg1	activities					1470:1479	anti-inflammatory activities	1452:1479	anti-inflammatory activities	1452:1479	To summarize, SSPs benefit intestinal health in C57BL/6J mice via a mechanism that involves elevating antioxidant and anti-inflammatory activities and regulating intestinal microbiota.
35359717	3	72	theme	polysaccharides	356:370	arg1	SSPs					373:376	S. spongiosa polysaccharides (SSPs)	343:377	S. spongiosa polysaccharides (SSPs)	343:377	Hence, our study aims to elaborate on the influences of S. spongiosa polysaccharides (SSPs) on antioxidant, anti-inflammatory, and intestinal microflora in C57BL/6J mice.
36965312	0	0	theme	assisted	88:95	arg1	extraction					97:106	Joint ultrasonic and enzyme assisted extraction	60:106	extraction	97:106	Antidiabetic activity of Armillaria mellea polysaccharides: Joint ultrasonic and enzyme assisted extraction.
36965312	5	1	from	levels	953:958	arg1	mice					972:975	diabetic mice	963:975	diabetic mice	963:975	UE-AMPs, when given by gavage, greatly prevented weight loss, increased water intake, and considerably decreased blood glucose levels in diabetic mice, which were dose-dependent (P < 0.05).
36965312	8	2	from	prospects	1474:1482	arg1	fields					1491:1496	the fields	1487:1496	the fields of natural medicine and functional food	1487:1536	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	0	3	theme	enzyme	81:86	arg1	extraction					97:106	Joint ultrasonic and enzyme assisted extraction	60:106	extraction	97:106	Antidiabetic activity of Armillaria mellea polysaccharides: Joint ultrasonic and enzyme assisted extraction.
36965312	1	4	theme	ultrasonic	167:176	arg1	U					199:199	U	199:199	U	199:199	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	4	theme	ultrasonic	167:176	arg1	extraction					187:196	ultrasonic assisted extraction	167:196	ultrasonic assisted extraction (U)	167:200	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	5	theme	ultrasonic-enzyme	238:254	arg1	UE					277:278	UE	277:278	UE	277:278	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	5	theme	ultrasonic-enzyme	238:254	arg1	extraction					265:274	ultrasonic-enzyme assisted extraction	238:274	ultrasonic-enzyme assisted extraction (UE)	238:279	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	3	6	theme	same	600:603	arg1	composition					620:630	the same monosaccharide composition	596:630	the same monosaccharide composition	596:630	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	2	7	theme	protein	505:511	arg1	content					513:519	the lowest protein content	494:519	the lowest protein content	494:519	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	5	8	theme	blood	939:943	arg1	levels					953:958	blood glucose levels	939:958	blood glucose levels	939:958	UE-AMPs, when given by gavage, greatly prevented weight loss, increased water intake, and considerably decreased blood glucose levels in diabetic mice, which were dose-dependent (P < 0.05).
36965312	5	9	theme	weight	875:880	arg1	loss					882:885	weight loss	875:885	weight loss	875:885	UE-AMPs, when given by gavage, greatly prevented weight loss, increased water intake, and considerably decreased blood glucose levels in diabetic mice, which were dose-dependent (P < 0.05).
36965312	1	10	theme	mellea	120:125	arg1	AMPs					144:147	AMPs	144:147	AMPs	144:147	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	10	theme	mellea	120:125	arg1	polysaccharides					127:141	Armillaria mellea polysaccharides	109:141	Armillaria mellea polysaccharides (AMPs)	109:148	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	11	theme	assisted	256:263	arg1	UE					277:278	UE	277:278	UE	277:278	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	11	theme	assisted	256:263	arg1	extraction					265:274	ultrasonic-enzyme assisted extraction	238:274	ultrasonic-enzyme assisted extraction (UE)	238:279	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	8	12	theme	natural	1501:1507	arg1	medicine					1509:1516	natural medicine	1501:1516	natural medicine	1501:1516	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	3	13	theme	different	639:647	arg1	proportions					649:659	different proportions	639:659	different proportions	639:659	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	2	14	theme	lowest	498:503	arg1	content					513:519	the lowest protein content	494:519	the lowest protein content	494:519	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	3	15	contain	have	682:685	arg1	UE-AMPs					671:677	UE-AMPs	671:677	UE-AMPs	671:677	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	3	15	contain	have	682:685	arg2	activity					715:722	the most potent antioxidant activity	687:722	the most potent antioxidant activity	687:722	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	8	16	theme	functional	1522:1531	arg1	food					1533:1536	functional food	1522:1536	functional food	1522:1536	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	6	17	theme	function	1138:1145	arg1	levels					1090:1095	lipid levels	1084:1095	lipid levels in the blood	1084:1108	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	17	theme	function	1138:1145	arg1	impairment					1147:1156	liver function impairment	1132:1156	liver function impairment	1132:1156	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	3	18	contain	had	592:594	arg1	AMPs					544:547	AMPs	544:547	AMPs obtained from the three extraction methods	544:590	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	3	18	contain	had	592:594	arg2	composition					620:630	the same monosaccharide composition	596:630	the same monosaccharide composition	596:630	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	1	19	theme	assisted	178:185	arg1	U					199:199	U	199:199	U	199:199	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	19	theme	assisted	178:185	arg1	extraction					187:196	ultrasonic assisted extraction	167:196	ultrasonic assisted extraction (U)	167:200	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	6	20	from	levels	1090:1095	arg1	blood					1104:1108	the blood	1100:1108	the blood	1100:1108	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	21	theme	liver	1132:1136	arg1	levels					1090:1095	lipid levels	1084:1095	lipid levels in the blood	1084:1108	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	21	theme	liver	1132:1136	arg1	impairment					1147:1156	liver function impairment	1132:1156	liver function impairment	1132:1156	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	22	from	reduction	1071:1079	arg1	blood					1104:1108	the blood	1100:1108	the blood	1100:1108	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	23	theme	positive	1048:1055	arg1	effect					1057:1062	a positive effect	1046:1062	a positive effect	1046:1062	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	2	24	theme	higher	347:352	arg1	times					341:345	1.64 times	336:345	1.64 times higher than that of U-AMPs (3.86 ± 0.11%)	336:387	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	2	24	theme	higher	347:352	arg1	yield					300:304	The yield	296:304	The yield of UE-AMPs (6.32 ± 0.14%)	296:330	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	0	25	theme	Antidiabetic	0:11	arg1	activity					13:20	Antidiabetic activity	0:20	Antidiabetic activity of Armillaria mellea polysaccharides	0:57	Antidiabetic activity of Armillaria mellea polysaccharides: Joint ultrasonic and enzyme assisted extraction.
36965312	7	26	theme	liver	1303:1307	arg1	complications					1288:1300	diabetic complications	1279:1300	diabetic complications (liver disease and nephropathy)	1279:1332	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	7	26	theme	liver	1303:1307	arg1	disease					1309:1315	liver disease	1303:1315	liver disease	1303:1315	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	0	27	theme	Armillaria	25:34	arg1	polysaccharides					43:57	Armillaria mellea polysaccharides	25:57	Armillaria mellea polysaccharides	25:57	Antidiabetic activity of Armillaria mellea polysaccharides: Joint ultrasonic and enzyme assisted extraction.
36965312	2	28	theme	total	470:474	arg1	content					482:488	the highest total sugar content	458:488	the highest total sugar content	458:488	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	1	29	theme	Armillaria	109:118	arg1	AMPs					144:147	AMPs	144:147	AMPs	144:147	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	29	theme	Armillaria	109:118	arg1	polysaccharides					127:141	Armillaria mellea polysaccharides	109:141	Armillaria mellea polysaccharides (AMPs)	109:148	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	7	30	theme	mice	1269:1272	arg1	organs					1259:1264	the organs	1255:1264	the organs of mice	1255:1272	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	7	31	theme	hematoxylin-eosin	1191:1207	arg1	HE					1219:1220	HE	1219:1220	HE	1219:1220	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	7	31	theme	hematoxylin-eosin	1191:1207	arg1	staining					1209:1216	hematoxylin-eosin staining	1191:1216	hematoxylin-eosin staining (HE)	1191:1221	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	2	32	theme	highest	462:468	arg1	content					482:488	the highest total sugar content	458:488	the highest total sugar content	458:488	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	0	33	dep	ultrasonic	66:75	arg1	activity					13:20	Antidiabetic activity	0:20	Antidiabetic activity of Armillaria mellea polysaccharides	0:57	Antidiabetic activity of Armillaria mellea polysaccharides: Joint ultrasonic and enzyme assisted extraction.
36965312	0	34	theme	polysaccharides	43:57	arg1	activity					13:20	Antidiabetic activity	0:20	Antidiabetic activity of Armillaria mellea polysaccharides	0:57	Antidiabetic activity of Armillaria mellea polysaccharides: Joint ultrasonic and enzyme assisted extraction.
36965312	7	35	theme	pathological	1163:1174	arg1	observation					1176:1186	The pathological observation	1159:1186	The pathological observation by hematoxylin-eosin staining (HE)	1159:1221	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	6	36	theme	oxidative	1111:1119	arg1	levels					1090:1095	lipid levels	1084:1095	lipid levels in the blood	1084:1108	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	36	theme	oxidative	1111:1119	arg1	damage					1121:1126	oxidative damage	1111:1126	oxidative damage	1111:1126	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	1	37	theme	enzyme	203:208	arg1	E					231:231	E	231:231	E	231:231	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	37	theme	enzyme	203:208	arg1	extraction					219:228	enzyme assisted extraction	203:228	enzyme assisted extraction (E)	203:232	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	0	38	theme	mellea	36:41	arg1	polysaccharides					43:57	Armillaria mellea polysaccharides	25:57	Armillaria mellea polysaccharides	25:57	Antidiabetic activity of Armillaria mellea polysaccharides: Joint ultrasonic and enzyme assisted extraction.
36965312	8	39	theme	application	1462:1472	arg1	prospects					1474:1482	great application prospects	1456:1482	great application prospects in the fields of natural medicine and functional food	1456:1536	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	6	40	contain	had	1042:1044	arg2	effect					1057:1062	a positive effect	1046:1062	a positive effect	1046:1062	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	40	contain	had	1042:1044	arg1	UE-AMPs					1029:1035	UE-AMPs	1029:1035	UE-AMPs	1029:1035	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	4	41	theme	antidiabetic	729:740	arg1	activity					742:749	The antidiabetic activity	725:749	The antidiabetic activity of UE-AMPs	725:760	The antidiabetic activity of UE-AMPs was investigated in streptozotocin (STZ)-induced diabetic mice.
36965312	1	42	theme	assisted	210:217	arg1	E					231:231	E	231:231	E	231:231	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	1	42	theme	assisted	210:217	arg1	extraction					219:228	enzyme assisted extraction	203:228	enzyme assisted extraction (E)	203:232	Armillaria mellea polysaccharides (AMPs) were obtained by ultrasonic assisted extraction (U), enzyme assisted extraction (E) and ultrasonic-enzyme assisted extraction (UE), respectively.
36965312	2	43	theme	higher	404:409	arg1	times					398:402	1.21 times	393:402	1.21 times higher than that of E-AMPs (5.21 ± 0.09%)	393:444	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	3	44	theme	potent	696:701	arg1	activity					715:722	the most potent antioxidant activity	687:722	the most potent antioxidant activity	687:722	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	4	45	theme	UE-AMPs	754:760	arg1	activity					742:749	The antidiabetic activity	725:749	The antidiabetic activity of UE-AMPs	725:760	The antidiabetic activity of UE-AMPs was investigated in streptozotocin (STZ)-induced diabetic mice.
36965312	8	46	theme	suitable	1386:1393	arg1	choice					1395:1400	a suitable choice	1384:1400	a suitable choice for improving diabetes and its complications	1384:1445	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	8	46	theme	suitable	1386:1393	arg1	UE-AMPs					1372:1378	UE-AMPs	1372:1378	UE-AMPs	1372:1378	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	4	47	theme	diabetic	811:818	arg1	mice					820:823	streptozotocin (STZ)-induced diabetic mice	782:823	streptozotocin (STZ)-induced diabetic mice	782:823	The antidiabetic activity of UE-AMPs was investigated in streptozotocin (STZ)-induced diabetic mice.
36965312	5	48	theme	glucose	945:951	arg1	levels					953:958	blood glucose levels	939:958	blood glucose levels	939:958	UE-AMPs, when given by gavage, greatly prevented weight loss, increased water intake, and considerably decreased blood glucose levels in diabetic mice, which were dose-dependent (P < 0.05).
36965312	4	49	theme	-induced	802:809	arg1	mice					820:823	streptozotocin (STZ)-induced diabetic mice	782:823	streptozotocin (STZ)-induced diabetic mice	782:823	The antidiabetic activity of UE-AMPs was investigated in streptozotocin (STZ)-induced diabetic mice.
36965312	6	50	from	blood	1104:1108	arg1	reduction					1071:1079	the reduction	1067:1079	the reduction of lipid levels in the blood, oxidative damage and liver function impairment	1067:1156	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	8	51	theme	medicine	1509:1516	arg1	fields					1491:1496	the fields	1487:1496	the fields of natural medicine and functional food	1487:1536	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	5	52	theme	water	898:902	arg1	intake					904:909	water intake	898:909	water intake	898:909	UE-AMPs, when given by gavage, greatly prevented weight loss, increased water intake, and considerably decreased blood glucose levels in diabetic mice, which were dose-dependent (P < 0.05).
36965312	2	53	theme	UE-AMPs	309:315	arg1	times					341:345	1.64 times	336:345	1.64 times higher than that of U-AMPs (3.86 ± 0.11%)	336:387	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	2	53	theme	UE-AMPs	309:315	arg1	yield					300:304	The yield	296:304	The yield of UE-AMPs (6.32 ± 0.14%)	296:330	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	7	54	theme	diabetic	1279:1286	arg1	nephropathy					1321:1331	nephropathy	1321:1331	nephropathy	1321:1331	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	7	54	theme	diabetic	1279:1286	arg1	complications					1288:1300	diabetic complications	1279:1300	diabetic complications (liver disease and nephropathy)	1279:1332	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	7	54	theme	diabetic	1279:1286	arg1	disease					1309:1315	liver disease	1303:1315	liver disease	1303:1315	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	2	55	theme	sugar	476:480	arg1	content					482:488	the highest total sugar content	458:488	the highest total sugar content	458:488	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	5	56	theme	diabetic	963:970	arg1	mice					972:975	diabetic mice	963:975	diabetic mice	963:975	UE-AMPs, when given by gavage, greatly prevented weight loss, increased water intake, and considerably decreased blood glucose levels in diabetic mice, which were dose-dependent (P < 0.05).
36965312	6	57	theme	levels	1090:1095	arg1	reduction					1071:1079	the reduction	1067:1079	the reduction of lipid levels in the blood, oxidative damage and liver function impairment	1067:1156	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	2	58	located	found	526:530	arg2	meanwhile					447:455	meanwhile	447:455	meanwhile	447:455	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	2	58	located	found	526:530	arg2	content					482:488	the highest total sugar content	458:488	the highest total sugar content	458:488	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	2	58	located	found	526:530	arg1	UE-AMPs					535:541	UE-AMPs	535:541	UE-AMPs	535:541	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	2	58	located	found	526:530	arg2	content					513:519	the lowest protein content	494:519	the lowest protein content	494:519	The yield of UE-AMPs (6.32 ± 0.14%) was 1.64 times higher than that of U-AMPs (3.86 ± 0.11%) and 1.21 times higher than that of E-AMPs (5.21 ± 0.09%); meanwhile, the highest total sugar content and the lowest protein content were found in UE-AMPs.
36965312	5	59	dep	dose-dependent	989:1002	arg1	P < 0.05					1005:1012	P < 0.05	1005:1012	P < 0.05	1005:1012	UE-AMPs, when given by gavage, greatly prevented weight loss, increased water intake, and considerably decreased blood glucose levels in diabetic mice, which were dose-dependent (P < 0.05).
36965312	3	60	theme	extraction	573:582	arg1	methods					584:590	the three extraction methods	563:590	the three extraction methods	563:590	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	6	61	theme	lipid	1084:1088	arg1	levels					1090:1095	lipid levels	1084:1095	lipid levels in the blood	1084:1108	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	61	theme	lipid	1084:1088	arg1	damage					1121:1126	oxidative damage	1111:1126	oxidative damage	1111:1126	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	6	61	theme	lipid	1084:1088	arg1	impairment					1147:1156	liver function impairment	1132:1156	liver function impairment	1132:1156	In addition, UE-AMPs also had a positive effect on the reduction of lipid levels in the blood, oxidative damage and liver function impairment.
36965312	8	62	contain	have	1451:1454	arg1	choice					1395:1400	a suitable choice	1384:1400	a suitable choice for improving diabetes and its complications	1384:1445	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	8	62	contain	have	1451:1454	arg2	prospects					1474:1482	great application prospects	1456:1482	great application prospects in the fields of natural medicine and functional food	1456:1536	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	8	62	contain	have	1451:1454	arg1	UE-AMPs					1372:1378	UE-AMPs	1372:1378	UE-AMPs	1372:1378	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	3	63	theme	antioxidant	703:713	arg1	activity					715:722	the most potent antioxidant activity	687:722	the most potent antioxidant activity	687:722	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	8	64	theme	food	1533:1536	arg1	fields					1491:1496	the fields	1487:1496	the fields of natural medicine and functional food	1487:1536	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
36965312	3	65	theme	monosaccharide	605:618	arg1	composition					620:630	the same monosaccharide composition	596:630	the same monosaccharide composition	596:630	AMPs obtained from the three extraction methods had the same monosaccharide composition but in different proportions, allowing UE-AMPs to have the most potent antioxidant activity.
36965312	7	66	dep	complications	1288:1300	arg1	nephropathy					1321:1331	nephropathy	1321:1331	nephropathy	1321:1331	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	7	66	dep	complications	1288:1300	arg1	complications					1288:1300	diabetic complications	1279:1300	diabetic complications (liver disease and nephropathy)	1279:1332	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	7	66	dep	complications	1288:1300	arg1	disease					1309:1315	liver disease	1303:1315	liver disease	1303:1315	The pathological observation by hematoxylin-eosin staining (HE) revealed that UE-AMPs protected the organs of mice from diabetic complications (liver disease and nephropathy).
36965312	8	67	theme	great	1456:1460	arg1	prospects					1474:1482	great application prospects	1456:1482	great application prospects in the fields of natural medicine and functional food	1456:1536	Hence, our findings demonstrate that UE-AMPs are a suitable choice for improving diabetes and its complications and have great application prospects in the fields of natural medicine and functional food.
37025610	7	0	theme	IC50	1603:1606	arg1	values					1608:1613	IC50 values	1603:1613	IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1603:1729	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	2	1	theme	high-performance	785:800	arg1	HPLC					825:828	HPLC	825:828	HPLC	825:828	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	2	1	theme	high-performance	785:800	arg1	chromatography					809:822	high-performance liquid chromatography	785:822	high-performance liquid chromatography (HPLC)	785:829	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	1	2	theme	surface	619:625	arg1	methodology					627:637	response surface methodology	610:637	response surface methodology	610:637	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	0	3	theme	medicinal	175:183	arg1	values					185:190	high nutritional and medicinal values	154:190	high nutritional and medicinal values	154:190	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	1	4	theme	glycol	389:394	arg1	system					359:364	an aqueous two-phase system	338:364	an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)	338:400	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	4	theme	glycol	389:394	arg1	Methods					308:314	Methods	308:314	Methods In the present study	308:335	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	4	theme	glycol	389:394	arg1	ATPS					367:370	ATPS	367:370	ATPS	367:370	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	8	5	dep	Discussion	1732:1741	arg1	showed					1755:1760	showed	1755:1760	showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides	1755:1883	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	3	6	theme	in	903:904	arg1	experiment					924:933	in vitro antioxidant experiment	903:933	in vitro antioxidant experiment	903:933	At the same time, the antioxidant activity of ALPs was investigated by in vitro antioxidant experiment.
37025610	4	7	theme	optimized	948:956	arg1	parameters					969:978	The optimized extraction parameters	944:978	The optimized extraction parameters for extraction ALPs	944:998	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	8	theme	extraction	1136:1145	arg1	temperature					1147:1157	an extraction temperature	1133:1157	an extraction temperature of 80°C.	1133:1166	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	2	9	dep	transform	705:713	arg1	infrared					715:722	infrared	715:722	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC)	705:829	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	1	10	dep	Methods	308:314	arg1	optimized					558:566	optimized	558:566	were optimized by using the single-factor experiment and response surface methodology	553:637	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	4	11	theme	relative	1025:1032	arg1	weight					1044:1049	the PEG relative molecular weight	1017:1049	the PEG relative molecular weight of 6,000	1017:1058	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	1	12	theme	ALPs	548:551	arg1	conditions					528:537	the optimal extraction conditions	505:537	the optimal extraction conditions of crude ALPs	505:551	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	4	13	theme	ALPs	1215:1218	arg1	rate					1207:1210	the extraction rate	1192:1210	the extraction rate of ALPs	1192:1218	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	1	14	theme	NH4	403:405	arg1	2SO4					407:410	(NH4)2SO4	402:410	(NH4)2SO4	402:410	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	3	15	theme	antioxidant	912:922	arg1	experiment					924:933	in vitro antioxidant experiment	903:933	in vitro antioxidant experiment	903:933	At the same time, the antioxidant activity of ALPs was investigated by in vitro antioxidant experiment.
37025610	0	16	attach	derived	85:91	arg1	root					145:148	Arctium lappa L. Introduction Arctium lappa L. root	98:148	Arctium lappa L. Introduction Arctium lappa L. root	98:148	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	16	attach	derived	85:91	arg2	polysaccharides					69:83	polysaccharides	69:83	polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root	69:148	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	1	17	theme	aqueous	341:347	arg1	system					359:364	an aqueous two-phase system	338:364	an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)	338:400	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	17	theme	aqueous	341:347	arg1	Methods					308:314	Methods	308:314	Methods In the present study	308:335	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	17	theme	aqueous	341:347	arg1	ATPS					367:370	ATPS	367:370	ATPS	367:370	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	7	18	theme	scavenging	1710:1719	arg1	abilities					1721:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	0	19	theme	L.	142:143	arg1	root					145:148	Arctium lappa L. Introduction Arctium lappa L. root	98:148	Arctium lappa L. Introduction Arctium lappa L. root	98:148	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	9	20	theme	functional	1945:1954	arg1	food					1956:1959	a functional food	1943:1959	a functional food	1943:1959	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	9	20	theme	functional	1945:1954	arg1	prospects					1930:1938	great prospects	1924:1938	great prospects	1924:1938	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	9	21	contain	had	1920:1922	arg2	food					1956:1959	a functional food	1943:1959	a functional food	1943:1959	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	9	21	contain	had	1920:1922	arg1	ALPs					1915:1918	ALPs	1915:1918	ALPs	1915:1918	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	9	21	contain	had	1920:1922	arg2	prospects					1930:1938	great prospects	1924:1938	great prospects	1924:1938	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	1	22	theme	single-factor	581:593	arg1	experiment					595:604	the single-factor experiment	577:604	the single-factor experiment	577:604	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	5	23	contain	had	1330:1332	arg2	surface					1401:1407	a rough surface	1393:1407	a rough surface	1393:1407	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	5	23	contain	had	1330:1332	arg2	distribution					1355:1366	uneven particle size distribution	1334:1366	uneven particle size distribution	1334:1366	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	5	23	contain	had	1330:1332	arg1	ALPs					1279:1282	ALPs	1279:1282	ALPs	1279:1282	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	5	23	contain	had	1330:1332	arg2	shape					1382:1386	an irregular shape	1369:1386	an irregular shape	1369:1386	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	0	24	theme	nutritional	159:169	arg1	values					185:190	high nutritional and medicinal values	154:190	high nutritional and medicinal values	154:190	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	25	theme	Republic	289:296	arg1	Health					266:271	Health	266:271	Health of the People's Republic of China	266:305	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	26	theme	China	301:305	arg1	Republic					289:296	the People's Republic	276:296	the People's Republic of China	276:305	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	4	27	theme	6,000	1054:1058	arg1	temperature					1147:1157	an extraction temperature	1133:1157	an extraction temperature of 80°C.	1133:1166	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	27	theme	6,000	1054:1058	arg1	fraction					1071:1078	a quality fraction	1061:1078	a quality fraction of PEG 25%	1061:1089	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	27	theme	6,000	1054:1058	arg1	weight					1044:1049	the PEG relative molecular weight	1017:1049	the PEG relative molecular weight of 6,000	1017:1058	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	27	theme	6,000	1054:1058	arg1	fraction					1102:1109	a quality fraction	1092:1109	a quality fraction of (NH4)2SO4 18%	1092:1126	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	1	28	theme	extraction	517:526	arg1	conditions					528:537	the optimal extraction conditions	505:537	the optimal extraction conditions of crude ALPs	505:551	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	0	29	theme	food	229:232	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	29	theme	food	229:232	arg1	material					238:245	a healthy food raw material	219:245	a healthy food raw material	219:245	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	5	30	theme	acidic	1297:1302	arg1	heteropolysaccharides					1304:1324	typical acidic heteropolysaccharides	1289:1324	typical acidic heteropolysaccharides	1289:1324	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	9	31	theme	multiple	1987:1994	arg1	fields					1996:2001	multiple fields	1987:2001	multiple fields	1987:2001	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	1	32	theme	present	323:329	arg1	study					331:335	the present study	319:335	the present study	319:335	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	7	33	theme	anion	1691:1695	arg1	abilities					1721:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	4	34	theme	PEG	1021:1023	arg1	weight					1044:1049	the PEG relative molecular weight	1017:1049	the PEG relative molecular weight of 6,000	1017:1058	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	2	35	dep	structure	644:652	arg1	The					640:642	The	640:642	The	640:642	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	8	36	theme	extract	1799:1805	arg1	polysaccharides					1807:1821	extract polysaccharides	1799:1821	extract polysaccharides	1799:1821	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	6	37	theme	molar	1495:1499	arg1	ratio					1501:1505	a molar ratio	1493:1505	a molar ratio of 70.19:10.95:11.16:6.90	1493:1531	The ALPs were chiefly composed of glucose, rhamnose, arabinose, and galactose with a molar ratio of 70.19:10.95:11.16:6.90.
37025610	4	38	theme	PEG	1083:1085	arg1	%					1089:1089	PEG 25%	1083:1089	PEG 25%	1083:1089	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	5	39	theme	size	1350:1353	arg1	distribution					1355:1366	uneven particle size distribution	1334:1366	uneven particle size distribution	1334:1366	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	4	40	theme	%	1089:1089	arg1	temperature					1147:1157	an extraction temperature	1133:1157	an extraction temperature of 80°C.	1133:1166	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	40	theme	%	1089:1089	arg1	fraction					1071:1078	a quality fraction	1061:1078	a quality fraction of PEG 25%	1061:1089	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	40	theme	%	1089:1089	arg1	weight					1044:1049	the PEG relative molecular weight	1017:1049	the PEG relative molecular weight of 6,000	1017:1058	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	40	theme	%	1089:1089	arg1	fraction					1102:1109	a quality fraction	1092:1109	a quality fraction of (NH4)2SO4 18%	1092:1126	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	0	41	theme	structural	12:21	arg1	characterization					23:38	structural characterization	12:38	structural characterization	12:38	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	8	42	used	used	1836:1839	arg2	method					1789:1794	an efficient method	1776:1794	an efficient method to extract polysaccharides	1776:1821	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	8	42	used	used	1836:1839	arg2	ATPS					1767:1770	ATPS	1767:1770	ATPS	1767:1770	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	2	43	theme	liquid	802:807	arg1	HPLC					825:828	HPLC	825:828	HPLC	825:828	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	2	43	theme	liquid	802:807	arg1	chromatography					809:822	high-performance liquid chromatography	785:822	high-performance liquid chromatography (HPLC)	785:829	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	1	44	theme	lappa	489:493	arg1	roots					498:502	the Arctium lappa L. roots	477:502	the Arctium lappa L. roots	477:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	7	45	theme	radical	1654:1660	arg1	abilities					1721:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	7	46	theme	radical	1626:1632	arg1	abilities					1721:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	1	47	from	Methods	308:314	arg1	study					331:335	the present study	319:335	the present study	319:335	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	3	48	theme	same	839:842	arg1	time					844:847	the same time	835:847	the same time	835:847	At the same time, the antioxidant activity of ALPs was investigated by in vitro antioxidant experiment.
37025610	5	49	theme	uneven	1334:1339	arg1	distribution					1355:1366	uneven particle size distribution	1334:1366	uneven particle size distribution	1334:1366	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	8	50	theme	other	1863:1867	arg1	polysaccharides					1869:1883	other polysaccharides	1863:1883	other polysaccharides	1863:1883	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	1	51	theme	Arctium	432:438	arg1	ALPs					466:469	ALPs	466:469	ALPs	466:469	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	51	theme	Arctium	432:438	arg1	polysaccharides					449:463	extract Arctium lappa L. polysaccharides	424:463	extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots	424:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	3	52	dep	in	903:904	arg1	vitro					906:910	vitro	906:910	vitro	906:910	At the same time, the antioxidant activity of ALPs was investigated by in vitro antioxidant experiment.
37025610	3	53	theme	antioxidant	854:864	arg1	activity					866:873	the antioxidant activity	850:873	the antioxidant activity of ALPs	850:881	At the same time, the antioxidant activity of ALPs was investigated by in vitro antioxidant experiment.
37025610	2	54	theme	scanning	745:752	arg1	SEM					775:777	SEM	775:777	SEM	775:777	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	2	54	theme	scanning	745:752	arg1	microscopy					763:772	scanning electron microscopy	745:772	scanning electron microscopy (SEM)	745:778	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	2	55	theme	electron	754:761	arg1	SEM					775:777	SEM	775:777	SEM	775:777	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	2	55	theme	electron	754:761	arg1	microscopy					763:772	scanning electron microscopy	745:772	scanning electron microscopy (SEM)	745:778	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	1	56	theme	response	610:617	arg1	methodology					627:637	response surface methodology	610:637	response surface methodology	610:637	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	4	57	theme	80°C.	1162:1166	arg1	temperature					1147:1157	an extraction temperature	1133:1157	an extraction temperature of 80°C.	1133:1166	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	57	theme	80°C.	1162:1166	arg1	fraction					1071:1078	a quality fraction	1061:1078	a quality fraction of PEG 25%	1061:1089	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	57	theme	80°C.	1162:1166	arg1	weight					1044:1049	the PEG relative molecular weight	1017:1049	the PEG relative molecular weight of 6,000	1017:1058	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	57	theme	80°C.	1162:1166	arg1	fraction					1102:1109	a quality fraction	1092:1109	a quality fraction of (NH4)2SO4 18%	1092:1126	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	1	58	theme	L.	446:447	arg1	ALPs					466:469	ALPs	466:469	ALPs	466:469	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	58	theme	L.	446:447	arg1	polysaccharides					449:463	extract Arctium lappa L. polysaccharides	424:463	extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots	424:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	4	59	theme	extraction	958:967	arg1	parameters					969:978	The optimized extraction parameters	944:978	The optimized extraction parameters for extraction ALPs	944:998	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	5	60	theme	irregular	1372:1380	arg1	shape					1382:1386	an irregular shape	1369:1386	an irregular shape	1369:1386	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	7	61	theme	intense	1560:1566	arg1	activity					1580:1587	intense antioxidant activity	1560:1587	intense antioxidant activity	1560:1587	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	5	62	theme	SEM	1246:1248	arg1	results					1259:1265	FTIR, SEM and HPLC results	1240:1265	FTIR, SEM and HPLC results	1240:1265	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	7	63	theme	antioxidant	1568:1578	arg1	activity					1580:1587	intense antioxidant activity	1560:1587	intense antioxidant activity	1560:1587	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	2	64	theme	ALPs	673:676	arg1	composition					658:668	composition	658:668	composition	658:668	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	2	64	theme	ALPs	673:676	arg1	structure					644:652	structure	644:652	structure	644:652	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	5	65	theme	HPLC	1254:1257	arg1	results					1259:1265	FTIR, SEM and HPLC results	1240:1265	FTIR, SEM and HPLC results	1240:1265	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	0	66	theme	antioxidant	45:55	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	4	67	theme	molecular	1034:1042	arg1	weight					1044:1049	the PEG relative molecular weight	1017:1049	the PEG relative molecular weight of 6,000	1017:1058	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	1	68	theme	crude	542:546	arg1	ALPs					548:551	crude ALPs	542:551	crude ALPs	542:551	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	5	69	theme	FTIR	1240:1243	arg1	results					1259:1265	FTIR, SEM and HPLC results	1240:1265	FTIR, SEM and HPLC results	1240:1265	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	1	70	used	used	416:419	arg2	2SO4					407:410	(NH4)2SO4	402:410	(NH4)2SO4	402:410	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	2	71	dep	fourier	697:703	arg1	transform					705:713	transform	705:713	transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC)	705:829	The structure and composition of ALPs were determined by fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and high-performance liquid chromatography (HPLC).
37025610	1	72	from	roots	498:502	arg1	ALPs					466:469	ALPs	466:469	ALPs	466:469	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	72	from	roots	498:502	arg1	polysaccharides					449:463	extract Arctium lappa L. polysaccharides	424:463	extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots	424:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	9	73	theme	great	1924:1928	arg1	food					1956:1959	a functional food	1943:1959	a functional food	1943:1959	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	9	73	theme	great	1924:1928	arg1	prospects					1930:1938	great prospects	1924:1938	great prospects	1924:1938	These results indicated that ALPs had great prospects as a functional food and could be exploited in multiple fields.
37025610	1	74	theme	two-phase	349:357	arg1	system					359:364	an aqueous two-phase system	338:364	an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)	338:400	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	74	theme	two-phase	349:357	arg1	Methods					308:314	Methods	308:314	Methods In the present study	308:335	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	74	theme	two-phase	349:357	arg1	ATPS					367:370	ATPS	367:370	ATPS	367:370	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	4	75	theme	quality	1063:1069	arg1	fraction					1071:1078	a quality fraction	1061:1078	a quality fraction of PEG 25%	1061:1089	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	0	76	theme	high	154:157	arg1	values					185:190	high nutritional and medicinal values	154:190	high nutritional and medicinal values	154:190	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	77	theme	Health	266:271	arg1	Ministry					254:261	the Ministry	250:261	the Ministry of Health of the People's Republic of China	250:305	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	1	78	theme	L.	495:496	arg1	roots					498:502	the Arctium lappa L. roots	477:502	the Arctium lappa L. roots	477:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	4	79	theme	extraction	1196:1205	arg1	rate					1207:1210	the extraction rate	1192:1210	the extraction rate of ALPs	1192:1218	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	0	80	theme	polysaccharides	69:83	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	80	theme	polysaccharides	69:83	arg1	characterization					23:38	structural characterization	12:38	structural characterization	12:38	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	80	theme	polysaccharides	69:83	arg1	material					238:245	a healthy food raw material	219:245	a healthy food raw material	219:245	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	80	theme	polysaccharides	69:83	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	1	81	theme	polyethylene	376:387	arg1	PEG					397:399	PEG	397:399	PEG	397:399	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	81	theme	polyethylene	376:387	arg1	glycol					389:394	polyethylene glycol	376:394	polyethylene glycol (PEG)	376:400	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	82	theme	optimal	509:515	arg1	conditions					528:537	the optimal extraction conditions	505:537	the optimal extraction conditions of crude ALPs	505:551	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	5	83	theme	typical	1289:1295	arg1	heteropolysaccharides					1304:1324	typical acidic heteropolysaccharides	1289:1324	typical acidic heteropolysaccharides	1289:1324	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	4	84	theme	quality	1094:1100	arg1	fraction					1102:1109	a quality fraction	1092:1109	a quality fraction of (NH4)2SO4 18%	1092:1126	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	7	85	theme	superoxide	1680:1689	arg1	anion					1691:1695	superoxide anion	1680:1695	superoxide anion (0.15 mg/ml)	1680:1708	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	7	85	theme	superoxide	1680:1689	arg1	mg/ml					1703:1707	0.15 mg/ml	1698:1707	0.15 mg/ml	1698:1707	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	0	86	theme	healthy	221:227	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	86	theme	healthy	221:227	arg1	material					238:245	a healthy food raw material	219:245	a healthy food raw material	219:245	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	6	87	theme	70.19:10.95:11.16:6.90	1510:1531	arg1	ratio					1501:1505	a molar ratio	1493:1505	a molar ratio of 70.19:10.95:11.16:6.90	1493:1531	The ALPs were chiefly composed of glucose, rhamnose, arabinose, and galactose with a molar ratio of 70.19:10.95:11.16:6.90.
37025610	0	88	theme	raw	234:236	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	88	theme	raw	234:236	arg1	material					238:245	a healthy food raw material	219:245	a healthy food raw material	219:245	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	89	contain	has	150:152	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	89	contain	has	150:152	arg1	characterization					23:38	structural characterization	12:38	structural characterization	12:38	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	89	contain	has	150:152	arg1	material					238:245	a healthy food raw material	219:245	a healthy food raw material	219:245	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	89	contain	has	150:152	arg2	values					185:190	high nutritional and medicinal values	154:190	high nutritional and medicinal values	154:190	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	0	89	contain	has	150:152	arg1	activity					57:64	antioxidant activity	45:64	antioxidant activity	45:64	Extraction, structural characterization, and antioxidant activity of polysaccharides derived from Arctium lappa L. Introduction Arctium lappa L. root has high nutritional and medicinal values and has been identified as a healthy food raw material by the Ministry of Health of the People's Republic of China.
37025610	7	90	from	values	1608:1613	arg1	abilities					1721:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities	1618:1729	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	4	91	dep	Results	936:942	arg1	were					1000:1003	were	1000:1003	were as follows	1000:1014	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	91	dep	Results	936:942	arg1	reach					1226:1230	reach	1226:1230	could reach 28.83%	1220:1237	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	7	92	contain	had	1556:1558	arg2	activity					1580:1587	intense antioxidant activity	1560:1587	intense antioxidant activity	1560:1587	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	7	92	contain	had	1556:1558	arg1	ALPs					1551:1554	the ALPs	1547:1554	the ALPs	1547:1554	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	4	93	theme	%	1126:1126	arg1	temperature					1147:1157	an extraction temperature	1133:1157	an extraction temperature of 80°C.	1133:1166	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	93	theme	%	1126:1126	arg1	fraction					1071:1078	a quality fraction	1061:1078	a quality fraction of PEG 25%	1061:1089	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	93	theme	%	1126:1126	arg1	weight					1044:1049	the PEG relative molecular weight	1017:1049	the PEG relative molecular weight of 6,000	1017:1058	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	93	theme	%	1126:1126	arg1	fraction					1102:1109	a quality fraction	1092:1109	a quality fraction of (NH4)2SO4 18%	1092:1126	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	1	94	theme	Arctium	481:487	arg1	roots					498:502	the Arctium lappa L. roots	477:502	the Arctium lappa L. roots	477:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	8	95	theme	efficient	1779:1787	arg1	ATPS					1767:1770	ATPS	1767:1770	ATPS	1767:1770	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	8	95	theme	efficient	1779:1787	arg1	method					1789:1794	an efficient method	1776:1794	an efficient method to extract polysaccharides	1776:1821	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	7	96	theme	DPPH	1649:1652	arg1	radical					1654:1660	DPPH radical	1649:1660	DPPH radical (0.29 mg/ml)	1649:1673	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	7	96	theme	DPPH	1649:1652	arg1	mg/ml					1668:1672	0.29 mg/ml	1663:1672	0.29 mg/ml	1663:1672	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	7	97	theme	·OH	1622:1624	arg1	radical					1626:1632	the ·OH radical	1618:1632	the ·OH radical (1.732 mg/ml)	1618:1646	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	7	97	theme	·OH	1622:1624	arg1	mg/ml					1641:1645	1.732 mg/ml	1635:1645	1.732 mg/ml	1635:1645	In addition, the ALPs had intense antioxidant activity in vitro with IC50 values in the ·OH radical (1.732 mg/ml), DPPH radical (0.29 mg/ml), and superoxide anion (0.15 mg/ml) scavenging abilities.
37025610	8	98	theme	polysaccharides	1869:1883	arg1	extraction					1849:1858	the extraction	1845:1858	the extraction of other polysaccharides	1845:1883	Discussion The results showed that ATPS was an efficient method to extract polysaccharides and could be used for the extraction of other polysaccharides.
37025610	5	99	theme	particle	1341:1348	arg1	distribution					1355:1366	uneven particle size distribution	1334:1366	uneven particle size distribution	1334:1366	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	4	100	theme	2SO4	1119:1122	arg1	%					1126:1126	(NH4)2SO4 18%	1114:1126	(NH4)2SO4 18%	1114:1126	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	4	100	theme	2SO4	1119:1122	arg1	NH4					1115:1117	NH4	1115:1117	NH4	1115:1117	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	1	101	theme	extract	424:430	arg1	ALPs					466:469	ALPs	466:469	ALPs	466:469	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	101	theme	extract	424:430	arg1	polysaccharides					449:463	extract Arctium lappa L. polysaccharides	424:463	extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots	424:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	3	102	theme	ALPs	878:881	arg1	activity					866:873	the antioxidant activity	850:873	the antioxidant activity of ALPs	850:881	At the same time, the antioxidant activity of ALPs was investigated by in vitro antioxidant experiment.
37025610	4	103	theme	extraction	984:993	arg1	ALPs					995:998	extraction ALPs	984:998	extraction ALPs	984:998	Results The optimized extraction parameters for extraction ALPs were as follows: the PEG relative molecular weight of 6,000, a quality fraction of PEG 25%, a quality fraction of (NH4)2SO4 18%, and an extraction temperature of 80°C. Under these conditions, the extraction rate of ALPs could reach 28.83%.
37025610	5	104	theme	rough	1395:1399	arg1	surface					1401:1407	a rough surface	1393:1407	a rough surface	1393:1407	FTIR, SEM and HPLC results showed that ALPs were typical acidic heteropolysaccharides and had uneven particle size distribution, an irregular shape, and a rough surface.
37025610	1	105	theme	lappa	440:444	arg1	ALPs					466:469	ALPs	466:469	ALPs	466:469	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
37025610	1	105	theme	lappa	440:444	arg1	polysaccharides					449:463	extract Arctium lappa L. polysaccharides	424:463	extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots	424:502	Methods In the present study, an aqueous two-phase system (ATPS) of polyethylene glycol (PEG)-(NH4)2SO4 was used to extract Arctium lappa L. polysaccharides (ALPs) from the Arctium lappa L. roots, the optimal extraction conditions of crude ALPs were optimized by using the single-factor experiment and response surface methodology.
35085504	0	0	theme	gut	60:62	arg1	microbiota					64:73	the gut microbiota	56:73	the gut microbiota	56:73	Diet leaves a genetic signature in a keystone member of the gut microbiota.
35085504	6	1	theme	genetic	1111:1117	arg1	diversity					1119:1127	B. thetaiotaomicron genetic diversity	1091:1127	B. thetaiotaomicron genetic diversity	1091:1127	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	6	1	theme	genetic	1111:1117	arg1	biomarker					1140:1148	a biomarker	1138:1148	a biomarker for dietary differences among individuals	1138:1190	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	5	2	theme	diversity	937:945	arg1	maintenance					894:904	the maintenance	890:904	the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens	890:983	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	2	3	from	effects	273:279	arg1	composition					314:324	microbiota composition	303:324	microbiota composition	303:324	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	2	3	from	effects	273:279	arg1	functions					330:338	functions	330:338	functions	330:338	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	3	4	theme	prevalent	495:503	arg1	thetaiotaomicron					475:490	Bacteroides thetaiotaomicron	463:490	Bacteroides thetaiotaomicron	463:490	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	3	4	theme	prevalent	495:503	arg1	member					532:537	a prevalent fiber-degrading microbiota member	493:537	a prevalent fiber-degrading microbiota member	493:537	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	5	5	theme	Periodic	754:761	arg1	changes					771:777	Periodic dietary changes	754:777	Periodic dietary changes	754:777	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	5	6	theme	genetic	929:935	arg1	diversity					937:945	higher intraspecies genetic diversity	909:945	higher intraspecies genetic diversity compared to constant dietary regimens	909:983	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	6	7	theme	genetic	1034:1040	arg1	signature					1042:1050	a genetic signature	1032:1050	a genetic signature	1032:1050	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	0	8	theme	microbiota	64:73	arg1	member					46:51	a keystone member	35:51	a keystone member of the gut microbiota	35:73	Diet leaves a genetic signature in a keystone member of the gut microbiota.
35085504	1	9	from	diet	116:119	arg1	Switching					76:84	Switching	76:84	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet	76:167	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	6	10	theme	dietary	1154:1160	arg1	differences					1162:1172	dietary differences	1154:1172	dietary differences among individuals	1154:1190	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	3	11	theme	Bacteroides	463:473	arg1	thetaiotaomicron					475:490	Bacteroides thetaiotaomicron	463:490	Bacteroides thetaiotaomicron	463:490	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	3	11	theme	Bacteroides	463:473	arg1	member					532:537	a prevalent fiber-degrading microbiota member	493:537	a prevalent fiber-degrading microbiota member	493:537	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	3	12	theme	mutations	450:458	arg1	emergence					437:445	the emergence	433:445	the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut	433:574	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	4	13	link	mucin-derived	731:743	arg1	glycans					745:751	mucin-derived glycans	731:751	mucin-derived glycans	731:751	B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans.
35085504	5	14	theme	dietary	968:974	arg1	regimens					976:983	constant dietary regimens	959:983	constant dietary regimens	959:983	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	1	15	theme	high-fat	140:147	arg1	diet					164:167	a Western-style high-fat and high-sugar diet	124:167	a Western-style high-fat and high-sugar diet	124:167	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	4	16	theme	Western-style	661:673	arg1	diet					675:678	Western-style diet	661:678	Western-style diet	661:678	B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans.
35085504	2	17	theme	changes	292:298	arg1	effects					273:279	the effects	269:279	the effects of dietary changes on microbiota composition and functions	269:338	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	1	18	theme	high-sugar	153:162	arg1	diet					164:167	a Western-style high-fat and high-sugar diet	124:167	a Western-style high-fat and high-sugar diet	124:167	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	4	19	theme	glycans	745:751	arg1	degradation					716:726	degradation	716:726	degradation of mucin-derived glycans	716:751	B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans.
35085504	3	20	from	thetaiotaomicron	475:490	arg1	emergence					437:445	the emergence	433:445	the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut	433:574	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	2	21	theme	dietary	284:290	arg1	changes					292:298	dietary changes	284:298	dietary changes	284:298	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	5	22	theme	higher	909:914	arg1	diversity					937:945	higher intraspecies genetic diversity	909:945	higher intraspecies genetic diversity compared to constant dietary regimens	909:983	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	0	23	theme	genetic	14:20	arg1	signature					22:30	a genetic signature	12:30	a genetic signature	12:30	Diet leaves a genetic signature in a keystone member of the gut microbiota.
35085504	1	24	from	low-fat	93:99	arg1	Switching					76:84	Switching	76:84	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet	76:167	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	6	25	theme	microbiome	1055:1064	arg1	members					1066:1072	microbiome members	1055:1072	microbiome members	1055:1072	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	3	26	theme	gut	572:574	arg1	colonization					545:556	colonization	545:556	colonization of the murine gut	545:574	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	6	27	theme	dietary	1010:1016	arg1	changes					1018:1024	dietary changes	1010:1024	dietary changes	1010:1024	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	1	28	theme	microbiota	176:185	arg1	imbalances					187:196	microbiota imbalances	176:196	microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis)	176:257	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	6	29	theme	B. thetaiotaomicron	1091:1109	arg1	diversity					1119:1127	B. thetaiotaomicron genetic diversity	1091:1127	B. thetaiotaomicron genetic diversity	1091:1127	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	6	29	theme	B. thetaiotaomicron	1091:1109	arg1	biomarker					1140:1148	a biomarker	1138:1148	a biomarker for dietary differences among individuals	1138:1190	These results show that dietary changes leave a genetic signature in microbiome members and suggest that B. thetaiotaomicron genetic diversity could be a biomarker for dietary differences among individuals.
35085504	3	30	theme	dietary	592:598	arg1	regimens					600:607	different dietary regimens	582:607	different dietary regimens	582:607	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	2	31	theme	bacterial	381:389	arg1	evolution					391:399	gut bacterial evolution	377:399	gut bacterial evolution	377:399	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	5	32	theme	such	819:822	arg1	mutations					824:832	such mutations	819:832	such mutations	819:832	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	4	33	dep	gut	653:655	arg1	the					649:651	the	649:651	the	649:651	B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans.
35085504	2	34	theme	gut	377:379	arg1	evolution					391:399	gut bacterial evolution	377:399	gut bacterial evolution	377:399	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	0	35	theme	keystone	37:44	arg1	member					46:51	a keystone member	35:51	a keystone member of the gut microbiota	35:73	Diet leaves a genetic signature in a keystone member of the gut microbiota.
35085504	5	36	theme	intraspecies	916:927	arg1	diversity					937:945	higher intraspecies genetic diversity	909:945	higher intraspecies genetic diversity compared to constant dietary regimens	909:983	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	3	37	from	emergence	437:445	arg1	thetaiotaomicron					475:490	Bacteroides thetaiotaomicron	463:490	Bacteroides thetaiotaomicron	463:490	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	3	37	from	emergence	437:445	arg1	member					532:537	a prevalent fiber-degrading microbiota member	493:537	a prevalent fiber-degrading microbiota member	493:537	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	4	38	theme	mucin-derived	731:743	arg1	glycans					745:751	mucin-derived glycans	731:751	mucin-derived glycans	731:751	B. thetaiotaomicron evolved rapidly in the gut and Western-style diet selected for mutations that promote degradation of mucin-derived glycans.
35085504	3	39	theme	different	582:590	arg1	regimens					600:607	different dietary regimens	582:607	different dietary regimens	582:607	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	5	40	theme	constant	959:966	arg1	regimens					976:983	constant dietary regimens	959:983	constant dietary regimens	959:983	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	3	41	theme	murine	565:570	arg1	gut					572:574	the murine gut	561:574	the murine gut	561:574	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	1	42	dep	dysbiosis	248:256	arg1	i.e.					242:245	i.e.	242:245	i.e.	242:245	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	2	43	from	impact	367:372	arg1	evolution					391:399	gut bacterial evolution	377:399	gut bacterial evolution	377:399	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	2	44	theme	microbiota	303:312	arg1	composition					314:324	microbiota composition	303:324	microbiota composition	303:324	Although the effects of dietary changes on microbiota composition and functions are well documented, their impact in gut bacterial evolution remains unexplored.
35085504	3	45	from	mutations	450:458	arg1	thetaiotaomicron					475:490	Bacteroides thetaiotaomicron	463:490	Bacteroides thetaiotaomicron	463:490	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	3	45	from	mutations	450:458	arg1	member					532:537	a prevalent fiber-degrading microbiota member	493:537	a prevalent fiber-degrading microbiota member	493:537	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	5	46	from	fluctuations	786:797	arg1	frequency					806:814	the frequency	802:814	the frequency of such mutations	802:832	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	1	47	theme	high-fiber	105:114	arg1	diet					116:119	high-fiber diet	105:119	high-fiber diet	105:119	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	1	48	theme	many	212:215	arg1	conditions					230:239	many pathological conditions	212:239	many pathological conditions (i.e., dysbiosis)	212:257	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	1	48	theme	many	212:215	arg1	dysbiosis					248:256	dysbiosis	248:256	dysbiosis	248:256	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	3	49	theme	fiber-degrading	505:519	arg1	thetaiotaomicron					475:490	Bacteroides thetaiotaomicron	463:490	Bacteroides thetaiotaomicron	463:490	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	3	49	theme	fiber-degrading	505:519	arg1	member					532:537	a prevalent fiber-degrading microbiota member	493:537	a prevalent fiber-degrading microbiota member	493:537	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	1	50	theme	pathological	217:228	arg1	conditions					230:239	many pathological conditions	212:239	many pathological conditions (i.e., dysbiosis)	212:257	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	1	50	theme	pathological	217:228	arg1	dysbiosis					248:256	dysbiosis	248:256	dysbiosis	248:256	Switching from a low-fat and high-fiber diet to a Western-style high-fat and high-sugar diet causes microbiota imbalances that underlay many pathological conditions (i.e., dysbiosis).
35085504	5	51	theme	mutations	824:832	arg1	frequency					806:814	the frequency	802:814	the frequency of such mutations	802:832	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	5	52	theme	metabolic	859:867	arg1	shifts					869:874	metabolic shifts	859:874	metabolic shifts	859:874	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35085504	3	53	theme	microbiota	521:530	arg1	thetaiotaomicron					475:490	Bacteroides thetaiotaomicron	463:490	Bacteroides thetaiotaomicron	463:490	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	3	53	theme	microbiota	521:530	arg1	member					532:537	a prevalent fiber-degrading microbiota member	493:537	a prevalent fiber-degrading microbiota member	493:537	We followed the emergence of mutations in Bacteroides thetaiotaomicron, a prevalent fiber-degrading microbiota member, upon colonization of the murine gut under different dietary regimens.
35085504	5	54	theme	dietary	763:769	arg1	changes					771:777	Periodic dietary changes	754:777	Periodic dietary changes	754:777	Periodic dietary changes caused fluctuations in the frequency of such mutations and were associated with metabolic shifts, resulting in the maintenance of higher intraspecies genetic diversity compared to constant dietary regimens.
35575345	7	0	theme	mice	1474:1477	arg1	kidney					1460:1465	the kidney	1456:1465	the kidney of AKI mice	1456:1477	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	9	1	theme	acute	1902:1906	arg1	injury					1915:1920	drug-induced acute kidney injury	1889:1920	drug-induced acute kidney injury	1889:1920	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	0	2	from	pathway	227:233	arg1	mice					238:241	mice	238:241	mice	238:241	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	1	3	theme	curcumin	305:312	arg1	effects					294:300	the effects	290:300	the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI)	290:390	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	2	4	theme	acute	592:596	arg1	injury					605:610	acute kidney injury	592:610	acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	592:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	7	5	theme	MDA	1417:1419	arg1	content					1421:1427	the MDA content	1413:1427	the MDA content	1413:1427	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	8	6	theme	PI3K/Akt/NF-κB	1714:1727	arg1	pathway					1739:1745	the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway	1688:1745	pathway	1739:1745	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35575345	6	7	theme	diester	1222:1228	arg1	treatment					1230:1238	the DHA-acylated Cur diester treatment	1201:1238	the DHA-acylated Cur diester treatment	1201:1238	Notably, we found that the DHA-acylated Cur diester treatment remarkably changed the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism.
35575345	7	8	theme	DHA-acylated	1371:1382	arg1	diesters					1388:1395	dietary DHA-acylated Cur diesters	1363:1395	dietary DHA-acylated Cur diesters	1363:1395	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	5	9	theme	DHA-acylated	1077:1088	arg1	diester					1094:1100	the DHA-acylated Cur diester	1073:1100	the DHA-acylated Cur diester	1073:1100	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	1	10	theme	studies	268:274	arg1	number					258:263	An increasing number	244:263	An increasing number of studies	244:274	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	2	11	theme	mg	694:695	arg1	kg-1					697:700	20 mg kg-1	691:700	20 mg kg-1	691:700	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	11	theme	mg	694:695	arg1	cisplatin					680:688	cisplatin	680:688	cisplatin (20 mg kg-1)	680:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	3	12	theme	Cur	745:747	arg1	diesters					749:756	the DHA-acylated Cur diesters	728:756	the DHA-acylated Cur diesters	728:756	The results showed that the DHA-acylated Cur diesters significantly decreased the abnormally increased blood urea nitrogen, creatinine, lipopolysaccharide (LPS) and trimethylamine-N-oxide (TMAO) in serum caused by AKI.
35575345	0	13	theme	trimethylamine-N-oxide-mediated	170:200	arg1	pathway					227:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	3	14	theme	increased	797:805	arg1	nitrogen					818:825	the abnormally increased blood urea nitrogen	782:825	the abnormally increased blood urea nitrogen	782:825	The results showed that the DHA-acylated Cur diesters significantly decreased the abnormally increased blood urea nitrogen, creatinine, lipopolysaccharide (LPS) and trimethylamine-N-oxide (TMAO) in serum caused by AKI.
35575345	6	15	theme	microbiota	1285:1294	arg1	metabolism					1341:1350	microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism	1285:1350	microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism	1285:1350	Notably, we found that the DHA-acylated Cur diester treatment remarkably changed the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism.
35575345	5	16	theme	protective	1052:1061	arg1	better					1106:1111	better	1106:1111	better	1106:1111	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	5	16	theme	protective	1052:1061	arg1	effect					1063:1068	The renal protective effect	1042:1068	The renal protective effect of the DHA-acylated Cur diester	1042:1100	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	4	17	theme	Cur	977:979	arg1	diesters					981:988	DHA-acylated Cur diesters	964:988	DHA-acylated Cur diesters	964:988	Histopathological results confirmed that DHA-acylated Cur diesters clearly reduced the degree of renal tubular injury.
35575345	0	18	from	effect	114:119	arg1	lipopolysaccharide-					146:164	lipopolysaccharide-	146:164	lipopolysaccharide-	146:164	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	0	18	from	effect	114:119	arg1	pathway					227:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	2	19	theme	comparative	427:437	arg1	investigation					439:451	a comparative investigation	425:451	a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	425:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	0	20	dep	lipopolysaccharide-	146:164	arg1	the					142:144	the	142:144	the	142:144	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	2	21	theme	Cur	501:503	arg1	esters					505:510	dietary DHA-acylated Cur esters	480:510	dietary DHA-acylated Cur esters	480:510	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	1	22	theme	docosahexaenoic	324:338	arg1	DHA					346:348	DHA	346:348	DHA	346:348	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	1	22	theme	docosahexaenoic	324:338	arg1	acid					340:343	docosahexaenoic acid	324:343	docosahexaenoic acid (DHA)	324:349	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	0	23	theme	signaling	217:225	arg1	pathway					227:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	1	24	theme	kidney	372:377	arg1	AKI					387:389	AKI	387:389	AKI	387:389	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	1	24	theme	kidney	372:377	arg1	injury					379:384	acute kidney injury	366:384	acute kidney injury (AKI)	366:390	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	2	25	theme	Cur	534:536	arg1	esters					505:510	dietary DHA-acylated Cur esters	480:510	dietary DHA-acylated Cur esters	480:510	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	25	theme	Cur	534:536	arg1	recombination					543:555	recombination	543:555	recombination of curcumin and DHA	543:575	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	25	theme	Cur	534:536	arg1	derivatives					519:529	ester derivatives	513:529	ester derivatives of Cur	513:536	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	3	26	theme	urea	813:816	arg1	nitrogen					818:825	the abnormally increased blood urea nitrogen	782:825	the abnormally increased blood urea nitrogen	782:825	The results showed that the DHA-acylated Cur diesters significantly decreased the abnormally increased blood urea nitrogen, creatinine, lipopolysaccharide (LPS) and trimethylamine-N-oxide (TMAO) in serum caused by AKI.
35575345	5	27	theme	diester	1094:1100	arg1	better					1106:1111	better	1106:1111	better	1106:1111	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	5	27	theme	diester	1094:1100	arg1	effect					1063:1068	The renal protective effect	1042:1068	The renal protective effect of the DHA-acylated Cur diester	1042:1100	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	2	28	theme	derivatives	519:529	arg1	effect					470:475	the effect	466:475	the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	466:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	0	29	theme	gut	124:126	arg1	microbiota					128:137	gut microbiota	124:137	gut microbiota	124:137	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	4	30	theme	tubular	1026:1032	arg1	injury					1034:1039	renal tubular injury	1020:1039	renal tubular injury	1020:1039	Histopathological results confirmed that DHA-acylated Cur diesters clearly reduced the degree of renal tubular injury.
35575345	9	31	theme	above	1752:1756	arg1	results					1758:1764	The above results	1748:1764	The above results	1748:1764	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	0	32	theme	Docosahexaenoic	0:14	arg1	diester					39:45	Docosahexaenoic acid-acylated curcumin diester	0:45	Docosahexaenoic acid-acylated curcumin diester	0:45	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	5	33	theme	Cur	1165:1167	arg1	recombination					1148:1160	the recombination	1144:1160	the recombination of Cur and DHA	1144:1175	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	5	33	theme	Cur	1165:1167	arg1	monoester					1130:1138	the monoester	1126:1138	the monoester	1126:1138	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	9	34	theme	novel	1824:1828	arg1	candidate					1830:1838	a potentially novel candidate	1810:1838	a potentially novel candidate	1810:1838	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	9	34	theme	novel	1824:1828	arg1	diesters					1797:1804	DHA-acylated Cur diesters	1780:1804	DHA-acylated Cur diesters	1780:1804	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	2	35	theme	intraperitoneal	649:663	arg1	injection					665:673	a single intraperitoneal injection	640:673	a single intraperitoneal injection with cisplatin (20 mg kg-1)	640:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	0	36	theme	curcumin	30:37	arg1	diester					39:45	Docosahexaenoic acid-acylated curcumin diester	0:45	Docosahexaenoic acid-acylated curcumin diester	0:45	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	5	37	theme	DHA	1173:1175	arg1	recombination					1148:1160	the recombination	1144:1160	the recombination of Cur and DHA	1144:1175	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	5	37	theme	DHA	1173:1175	arg1	monoester					1130:1138	the monoester	1126:1138	the monoester	1126:1138	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	7	38	theme	fatty	1503:1507	arg1	composition					1514:1524	the fatty acid composition	1499:1524	the fatty acid composition in the kidney	1499:1538	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	2	39	theme	dietary	480:486	arg1	esters					505:510	dietary DHA-acylated Cur esters	480:510	dietary DHA-acylated Cur esters	480:510	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	9	40	theme	dietary	1852:1858	arg1	pattern					1860:1866	targeted dietary pattern	1843:1866	targeted dietary pattern	1843:1866	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	2	41	theme	esters	505:510	arg1	effect					470:475	the effect	466:475	the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	466:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	0	42	theme	acute	76:80	arg1	injury					89:94	cisplatin-induced acute kidney injury	58:94	cisplatin-induced acute kidney injury	58:94	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	8	43	theme	mechanism	1549:1557	arg1	studies					1559:1565	Further mechanism studies	1541:1565	Further mechanism studies	1541:1565	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35575345	0	44	from	lipopolysaccharide-	146:164	arg1	mice					238:241	mice	238:241	mice	238:241	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	7	45	theme	AKI	1470:1472	arg1	mice					1474:1477	AKI mice	1470:1477	AKI mice	1470:1477	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	9	46	theme	drug-induced	1889:1900	arg1	injury					1915:1920	drug-induced acute kidney injury	1889:1920	drug-induced acute kidney injury	1889:1920	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	8	47	theme	DHA-acylated	1579:1590	arg1	diesters					1596:1603	DHA-acylated Cur diesters	1579:1603	DHA-acylated Cur diesters	1579:1603	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35575345	2	48	theme	kidney	598:603	arg1	injury					605:610	acute kidney injury	592:610	acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	592:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	9	49	theme	kidney	1908:1913	arg1	injury					1915:1920	drug-induced acute kidney injury	1889:1920	drug-induced acute kidney injury	1889:1920	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	6	50	theme	relative	1263:1270	arg1	abundance					1272:1280	the relative abundance	1259:1280	the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism	1259:1350	Notably, we found that the DHA-acylated Cur diester treatment remarkably changed the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism.
35575345	7	51	theme	GSH	1442:1444	arg1	levels					1446:1451	GSH levels	1442:1451	GSH levels in the kidney of AKI mice	1442:1477	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	2	52	theme	mouse	617:621	arg1	model					623:627	a mouse model	615:627	a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	615:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	8	53	theme	TMAO-mediated	1700:1712	arg1	pathway					1739:1745	the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway	1688:1745	pathway	1739:1745	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35575345	7	54	from	levels	1446:1451	arg1	kidney					1460:1465	the kidney	1456:1465	the kidney of AKI mice	1456:1477	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	3	55	theme	DHA-acylated	732:743	arg1	diesters					749:756	the DHA-acylated Cur diesters	728:756	the DHA-acylated Cur diesters	728:756	The results showed that the DHA-acylated Cur diesters significantly decreased the abnormally increased blood urea nitrogen, creatinine, lipopolysaccharide (LPS) and trimethylamine-N-oxide (TMAO) in serum caused by AKI.
35575345	8	56	theme	signaling	1729:1737	arg1	pathway					1739:1745	the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway	1688:1745	pathway	1739:1745	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35575345	1	57	theme	increasing	247:256	arg1	number					258:263	An increasing number	244:263	An increasing number of studies	244:274	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	2	58	theme	recombination	543:555	arg1	effect					470:475	the effect	466:475	the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	466:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	6	59	theme	DHA-acylated	1205:1216	arg1	treatment					1230:1238	the DHA-acylated Cur diester treatment	1201:1238	the DHA-acylated Cur diester treatment	1201:1238	Notably, we found that the DHA-acylated Cur diester treatment remarkably changed the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism.
35575345	9	60	theme	DHA-acylated	1780:1791	arg1	candidate					1830:1838	a potentially novel candidate	1810:1838	a potentially novel candidate	1810:1838	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	9	60	theme	DHA-acylated	1780:1791	arg1	diesters					1797:1804	DHA-acylated Cur diesters	1780:1804	DHA-acylated Cur diesters	1780:1804	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	0	61	theme	PI3K/Akt/NF-κB	202:215	arg1	pathway					227:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway	170:233	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	5	62	theme	Cur	1090:1092	arg1	diester					1094:1100	the DHA-acylated Cur diester	1073:1100	the DHA-acylated Cur diester	1073:1100	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	2	63	theme	DHA	573:575	arg1	esters					505:510	dietary DHA-acylated Cur esters	480:510	dietary DHA-acylated Cur esters	480:510	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	63	theme	DHA	573:575	arg1	recombination					543:555	recombination	543:555	recombination of curcumin and DHA	543:575	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	63	theme	DHA	573:575	arg1	derivatives					519:529	ester derivatives	513:529	ester derivatives of Cur	513:536	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	7	64	theme	dietary	1363:1369	arg1	diesters					1388:1395	dietary DHA-acylated Cur diesters	1363:1395	dietary DHA-acylated Cur diesters	1363:1395	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	6	65	theme	related	1296:1302	arg1	metabolism					1341:1350	microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism	1285:1350	microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism	1285:1350	Notably, we found that the DHA-acylated Cur diester treatment remarkably changed the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism.
35575345	5	66	theme	renal	1046:1050	arg1	better					1106:1111	better	1106:1111	better	1106:1111	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	5	66	theme	renal	1046:1050	arg1	effect					1063:1068	The renal protective effect	1042:1068	The renal protective effect of the DHA-acylated Cur diester	1042:1100	The renal protective effect of the DHA-acylated Cur diester was better than that of the monoester and the recombination of Cur and DHA.
35575345	2	67	theme	curcumin	560:567	arg1	esters					505:510	dietary DHA-acylated Cur esters	480:510	dietary DHA-acylated Cur esters	480:510	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	67	theme	curcumin	560:567	arg1	recombination					543:555	recombination	543:555	recombination of curcumin and DHA	543:575	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	67	theme	curcumin	560:567	arg1	derivatives					519:529	ester derivatives	513:529	ester derivatives of Cur	513:536	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	3	68	theme	blood	807:811	arg1	nitrogen					818:825	the abnormally increased blood urea nitrogen	782:825	the abnormally increased blood urea nitrogen	782:825	The results showed that the DHA-acylated Cur diesters significantly decreased the abnormally increased blood urea nitrogen, creatinine, lipopolysaccharide (LPS) and trimethylamine-N-oxide (TMAO) in serum caused by AKI.
35575345	4	69	theme	DHA-acylated	964:975	arg1	diesters					981:988	DHA-acylated Cur diesters	964:988	DHA-acylated Cur diesters	964:988	Histopathological results confirmed that DHA-acylated Cur diesters clearly reduced the degree of renal tubular injury.
35575345	9	70	theme	Cur	1793:1795	arg1	candidate					1830:1838	a potentially novel candidate	1810:1838	a potentially novel candidate	1810:1838	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	9	70	theme	Cur	1793:1795	arg1	diesters					1797:1804	DHA-acylated Cur diesters	1780:1804	DHA-acylated Cur diesters	1780:1804	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	1	71	theme	acute	366:370	arg1	AKI					387:389	AKI	387:389	AKI	387:389	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	1	71	theme	acute	366:370	arg1	injury					379:384	acute kidney injury	366:384	acute kidney injury (AKI)	366:390	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	2	72	theme	DHA-acylated	488:499	arg1	esters					505:510	dietary DHA-acylated Cur esters	480:510	dietary DHA-acylated Cur esters	480:510	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	73	theme	single	642:647	arg1	injection					665:673	a single intraperitoneal injection	640:673	a single intraperitoneal injection with cisplatin (20 mg kg-1)	640:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	74	with	injection	665:673	arg1	kg-1					697:700	20 mg kg-1	691:700	20 mg kg-1	691:700	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	2	74	with	injection	665:673	arg1	cisplatin					680:688	cisplatin	680:688	cisplatin (20 mg kg-1)	680:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	4	75	theme	Histopathological	923:939	arg1	results					941:947	Histopathological results	923:947	Histopathological results	923:947	Histopathological results confirmed that DHA-acylated Cur diesters clearly reduced the degree of renal tubular injury.
35575345	0	76	theme	microbiota	128:137	arg1	effect					114:119	the effect	110:119	the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice	110:241	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	2	77	theme	ester	513:517	arg1	derivatives					519:529	ester derivatives	513:529	ester derivatives of Cur	513:536	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	6	78	theme	metabolism	1341:1350	arg1	abundance					1272:1280	the relative abundance	1259:1280	the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism	1259:1350	Notably, we found that the DHA-acylated Cur diester treatment remarkably changed the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism.
35575345	0	79	theme	acid-acylated	16:28	arg1	diester					39:45	Docosahexaenoic acid-acylated curcumin diester	0:45	Docosahexaenoic acid-acylated curcumin diester	0:45	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	4	80	theme	renal	1020:1024	arg1	injury					1034:1039	renal tubular injury	1020:1039	renal tubular injury	1020:1039	Histopathological results confirmed that DHA-acylated Cur diesters clearly reduced the degree of renal tubular injury.
35575345	7	81	from	composition	1514:1524	arg1	kidney					1533:1538	the kidney	1529:1538	the kidney	1529:1538	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	6	82	theme	Cur	1218:1220	arg1	treatment					1230:1238	the DHA-acylated Cur diester treatment	1201:1238	the DHA-acylated Cur diester treatment	1201:1238	Notably, we found that the DHA-acylated Cur diester treatment remarkably changed the relative abundance of microbiota related to LPS and TMAO/trimethylamine (TMA) metabolism.
35575345	4	83	theme	injury	1034:1039	arg1	degree					1010:1015	the degree	1006:1015	the degree of renal tubular injury	1006:1039	Histopathological results confirmed that DHA-acylated Cur diesters clearly reduced the degree of renal tubular injury.
35575345	2	84	from	injury	605:610	arg1	model					623:627	a mouse model	615:627	a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1)	615:701	In this work, we have performed a comparative investigation to determine the effect of dietary DHA-acylated Cur esters, ester derivatives of Cur, and recombination of curcumin and DHA on alleviating acute kidney injury in a mouse model induced by a single intraperitoneal injection with cisplatin (20 mg kg-1).
35575345	0	85	theme	cisplatin-induced	58:74	arg1	injury					89:94	cisplatin-induced acute kidney injury	58:94	cisplatin-induced acute kidney injury	58:94	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	1	86	theme	acid	340:343	arg1	effects					294:300	the effects	290:300	the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI)	290:390	An increasing number of studies have reported the effects of curcumin (Cur) and docosahexaenoic acid (DHA) on alleviating acute kidney injury (AKI).
35575345	7	87	theme	acid	1509:1512	arg1	composition					1514:1524	the fatty acid composition	1499:1524	the fatty acid composition in the kidney	1499:1538	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	9	88	theme	targeted	1843:1850	arg1	pattern					1860:1866	targeted dietary pattern	1843:1866	targeted dietary pattern	1843:1866	The above results indicate that DHA-acylated Cur diesters are a potentially novel candidate or targeted dietary pattern to prevent and treat drug-induced acute kidney injury.
35575345	8	89	theme	Further	1541:1547	arg1	studies					1559:1565	Further mechanism studies	1541:1565	Further mechanism studies	1541:1565	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35575345	0	90	theme	kidney	82:87	arg1	injury					89:94	cisplatin-induced acute kidney injury	58:94	cisplatin-induced acute kidney injury	58:94	Docosahexaenoic acid-acylated curcumin diester alleviates cisplatin-induced acute kidney injury by regulating the effect of gut microbiota on the lipopolysaccharide- and trimethylamine-N-oxide-mediated PI3K/Akt/NF-κB signaling pathway in mice.
35575345	7	91	theme	Cur	1384:1386	arg1	diesters					1388:1395	dietary DHA-acylated Cur diesters	1363:1395	dietary DHA-acylated Cur diesters	1363:1395	Moreover, dietary DHA-acylated Cur diesters clearly reduced the MDA content and elevated GSH levels in the kidney of AKI mice, as well as changed the fatty acid composition in the kidney.
35575345	8	92	theme	oxidative	1657:1665	arg1	stress					1667:1672	oxidative stress	1657:1672	oxidative stress	1657:1672	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35575345	8	93	theme	Cur	1592:1594	arg1	diesters					1596:1603	DHA-acylated Cur diesters	1579:1603	DHA-acylated Cur diesters	1579:1603	Further mechanism studies showed that DHA-acylated Cur diesters significantly inhibited inflammation, apoptosis and oxidative stress by preventing the LPS and TMAO-mediated PI3K/Akt/NF-κB signaling pathway.
35816772	5	0	theme	SJZD	1235:1238	arg1	contents					1178:1185	the contents	1174:1185	the contents of oligosaccharides and free monosaccharides of SJZD	1174:1238	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	3	1	theme	chromatographic	761:775	arg1	techniques					777:786	multiple chromatographic techniques	752:786	multiple chromatographic techniques	752:786	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	1	2	theme	traditional	269:279	arg1	Medicine					289:296	a traditional Chinese Medicine	267:296	a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc	267:374	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	6	3	theme	sulfur-fumigated	1290:1305	arg1	content					1307:1313	the sulfur-fumigated content	1286:1313	the sulfur-fumigated content of ginseng	1286:1324	The alterations of SJZD were aggravated with the sulfur-fumigated content of ginseng.
35816772	1	4	theme	Si-Jun-Zi	192:200	arg1	decoction					202:210	Si-Jun-Zi decoction	192:210	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb	192:262	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	1	4	theme	Si-Jun-Zi	192:200	arg1	SJZD					213:216	SJZD	213:216	SJZD	213:216	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	7	5	theme	pH	1570:1571	arg1	value					1573:1577	the pH value	1566:1577	the pH value	1566:1577	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	7	6	theme	SO2	1536:1538	arg1	SO2					1536:1538	SO2	1536:1538	SO2	1536:1538	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	7	6	theme	SO2	1536:1538	arg1	residue					1525:1531	the residue	1521:1531	the residue of SO2	1521:1538	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	9	7	theme	formulas	2056:2063	arg1	application					2034:2044	the application	2030:2044	the application of herbal formulas	2030:2063	In conclusion, sulfur-fumigated ginseng altered the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs.
35816772	8	8	theme	chemical	1871:1878	arg1	profiles					1880:1887	chemical profiles	1871:1887	chemical profiles in SJZD	1871:1895	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	3	9	theme	based	788:792	arg1	strategies					804:813	based glycomics strategies	788:813	based glycomics strategies	788:813	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	2	10	theme	chemical	562:569	arg1	composition					571:581	chemical composition	562:581	chemical composition	562:581	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	5	11	theme	oligosaccharides	1190:1205	arg1	contents					1178:1185	the contents	1174:1185	the contents of oligosaccharides and free monosaccharides of SJZD	1174:1238	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	4	12	from	emergence	884:892	arg1	SJZD					972:975	SJZD	972:975	SJZD	972:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	2	13	theme	medical	601:607	arg1	herbs					609:613	medical herbs	601:613	medical herbs	601:613	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	7	14	dep	sulfur-fumigation	1369:1385	arg1	1					1366:1366	1	1366:1366	1	1366:1366	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	7	15	dep	polysaccharides	1681:1695	arg1	to					1697:1698	to	1697:1698	to	1697:1698	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	7	15	dep	polysaccharides	1681:1695	arg1	oligosaccharides					1700:1715	oligosaccharides	1700:1715	oligosaccharides	1700:1715	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	3	16	theme	sulfur-fumigated	645:660	arg1	ginseng					662:668	sulfur-fumigated ginseng	645:668	sulfur-fumigated ginseng	645:668	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	0	17	dep	metabolomics	153:164	arg1	strategies					180:189	strategies	180:189	strategies	180:189	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	2	18	theme	sulfur-dioxide	529:542	arg1	residue					550:556	sulfur-dioxide (SO2) residue	529:556	sulfur-dioxide (SO2) residue	529:556	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	0	19	theme	decoction	71:79	arg1	quality					50:56	the global quality	39:56	the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription,	39:138	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	1	20	theme	cancer	365:370	arg1	etc					372:374	colorectal cancer etc	354:374	colorectal cancer etc	354:374	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	5	21	theme	saccharide	982:991	arg1	components					993:1002	saccharide components	982:1002	saccharide components	982:1002	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	0	22	from	Effects	0:6	arg1	quality					50:56	the global quality	39:56	the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription,	39:138	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	4	23	theme	non-saccharides	820:834	arg1	components					836:845	non-saccharides components	820:845	non-saccharides components	820:845	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	5	24	theme	free	1211:1214	arg1	monosaccharides					1216:1230	free monosaccharides	1211:1230	free monosaccharides	1211:1230	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	5	25	theme	molecular	1063:1071	arg1	weights					1073:1079	molecular weights	1063:1079	molecular weights	1063:1079	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	2	26	theme	SO2	545:547	arg1	residue					550:556	sulfur-dioxide (SO2) residue	529:556	sulfur-dioxide (SO2) residue	529:556	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	8	27	from	residue	1795:1801	arg1	ginseng					1823:1829	sulfur-fumigated ginseng	1806:1829	sulfur-fumigated ginseng	1806:1829	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	3	28	from	effect	635:640	arg1	quality					680:686	global quality	673:686	global quality of SJZD	673:694	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	0	29	theme	ginseng-containing	96:113	arg1	decoction					71:79	Si-Jun-Zi decoction	61:79	Si-Jun-Zi decoction	61:79	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	0	29	theme	ginseng-containing	96:113	arg1	prescription					126:137	a traditional ginseng-containing multi-herb prescription	82:137	a traditional ginseng-containing multi-herb prescription	82:137	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	9	30	theme	SJZD	1968:1971	arg1	quality					1957:1963	the global quality	1946:1963	the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs	1946:2102	In conclusion, sulfur-fumigated ginseng altered the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs.
35816772	7	31	from	polysaccharides	1681:1695	arg1	SJZD					1743:1746	SJZD	1743:1746	SJZD	1743:1746	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	1	32	theme	Chinese	281:287	arg1	Medicine					289:296	a traditional Chinese Medicine	267:296	a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc	267:374	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	0	33	theme	sulfur-fumigated	11:26	arg1	ginseng					28:34	sulfur-fumigated ginseng	11:34	sulfur-fumigated ginseng	11:34	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	1	34	theme	multi-herb	298:307	arg1	prescription					309:320	a traditional Chinese Medicine multi-herb prescription	267:320	a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc	267:374	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	2	35	theme	methods	500:506	arg1	one					466:468	one	466:468	one	466:468	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	2	35	theme	methods	500:506	arg1	methods					500:506	the postharvest processing methods	473:506	the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs	473:613	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	8	36	from	alterations	1856:1866	arg1	SJZD					1892:1895	SJZD	1892:1895	SJZD	1892:1895	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	2	37	theme	commercial	425:434	arg1	ginseng					436:442	the commercial ginseng	421:442	the commercial ginseng	421:442	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	2	38	theme	postharvest	477:487	arg1	methods					500:506	the postharvest processing methods	473:506	the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs	473:613	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	4	39	theme	sulfur-containing	897:913	arg1	derivatives					915:925	sulfur-containing derivatives	897:925	sulfur-containing derivatives	897:925	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	0	40	theme	global	43:48	arg1	quality					50:56	the global quality	39:56	the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription,	39:138	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	5	41	theme	polysaccharides	1143:1157	arg1	composition					1128:1138	the monosaccharide composition	1109:1138	the monosaccharide composition of polysaccharides	1109:1157	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	7	42	theme	sulfur-containing	1412:1428	arg1	derivatives					1430:1440	sulfur-containing derivatives	1412:1440	sulfur-containing derivatives	1412:1440	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	8	43	theme	SO2	1791:1793	arg1	residue					1795:1801	the SO2 residue	1787:1801	the SO2 residue in sulfur-fumigated ginseng	1787:1829	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	3	44	theme	SJZD	691:694	arg1	quality					680:686	global quality	673:686	global quality of SJZD	673:694	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	1	45	theme	principal	239:247	arg1	herb					259:262	the principal medicinal herb	235:262	the principal medicinal herb	235:262	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	2	46	from	composition	571:581	arg1	herbs					609:613	medical herbs	601:613	medical herbs	601:613	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	2	47	theme	Previous	377:384	arg1	studies					386:392	Previous studies	377:392	Previous studies	377:392	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	4	48	theme	saponins	945:952	arg1	alteration					931:940	alteration	931:940	alteration of saponins and flavonoids in SJZD	931:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	4	48	theme	saponins	945:952	arg1	derivatives					915:925	sulfur-containing derivatives	897:925	sulfur-containing derivatives	897:925	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	3	49	theme	UPLC-QTOF-MS/MS	713:727	arg1	metabolomics					735:746	UPLC-QTOF-MS/MS based metabolomics	713:746	UPLC-QTOF-MS/MS based metabolomics	713:746	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	4	50	theme	flavonoids	958:967	arg1	alteration					931:940	alteration	931:940	alteration of saponins and flavonoids in SJZD	931:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	4	50	theme	flavonoids	958:967	arg1	derivatives					915:925	sulfur-containing derivatives	897:925	sulfur-containing derivatives	897:925	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	7	51	from	degradation	1662:1672	arg1	SJZD					1743:1746	SJZD	1743:1746	SJZD	1743:1746	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	4	52	theme	derivatives	915:925	arg1	emergence					884:892	the emergence	880:892	the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD	880:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	9	53	theme	herbal	2049:2054	arg1	formulas					2056:2063	herbal formulas	2049:2063	herbal formulas	2049:2063	In conclusion, sulfur-fumigated ginseng altered the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs.
35816772	3	54	theme	multiple	752:759	arg1	techniques					777:786	multiple chromatographic techniques	752:786	multiple chromatographic techniques	752:786	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	7	55	dep	caused	1514:1519	arg1	2					1493:1493	2	1493:1493	2	1493:1493	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	4	56	theme	alteration	931:940	arg1	emergence					884:892	the emergence	880:892	the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD	880:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	8	57	from	SJZD	1892:1895	arg1	alterations					1856:1866	the alterations	1852:1866	the alterations of chemical profiles in SJZD	1852:1895	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	6	58	theme	SJZD	1260:1263	arg1	alterations					1245:1255	The alterations	1241:1255	The alterations of SJZD	1241:1263	The alterations of SJZD were aggravated with the sulfur-fumigated content of ginseng.
35816772	9	59	theme	sulfur-fumigated	2081:2096	arg1	herbs					2098:2102	sulfur-fumigated herbs	2081:2102	sulfur-fumigated herbs	2081:2102	In conclusion, sulfur-fumigated ginseng altered the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs.
35816772	3	60	theme	glycomics	794:802	arg1	strategies					804:813	based glycomics strategies	788:813	based glycomics strategies	788:813	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	8	61	theme	profiles	1880:1887	arg1	alterations					1856:1866	the alterations	1852:1866	the alterations of chemical profiles in SJZD	1852:1895	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	5	62	theme	sulfur-fumigated	1005:1020	arg1	ginseng					1022:1028	sulfur-fumigated ginseng	1005:1028	sulfur-fumigated ginseng	1005:1028	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	7	63	theme	monosaccharides	1724:1738	arg1	degradation					1662:1672	the degradation	1658:1672	the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD	1658:1746	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	2	64	from	residue	550:556	arg1	herbs					609:613	medical herbs	601:613	medical herbs	601:613	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	0	65	theme	Si-Jun-Zi	61:69	arg1	decoction					71:79	Si-Jun-Zi decoction	61:79	Si-Jun-Zi decoction	61:79	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	0	65	theme	Si-Jun-Zi	61:69	arg1	prescription					126:137	a traditional ginseng-containing multi-herb prescription	82:137	a traditional ginseng-containing multi-herb prescription	82:137	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	4	66	from	SJZD	972:975	arg1	emergence					884:892	the emergence	880:892	the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD	880:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	6	67	theme	ginseng	1318:1324	arg1	content					1307:1313	the sulfur-fumigated content	1286:1313	the sulfur-fumigated content of ginseng	1286:1324	The alterations of SJZD were aggravated with the sulfur-fumigated content of ginseng.
35816772	5	68	dep	contents	1050:1057	arg1	the					1040:1042	the	1040:1042	the	1040:1042	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	1	69	theme	colorectal	354:363	arg1	cancer					365:370	colorectal cancer	354:370	colorectal cancer etc	354:374	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	7	70	from	SJZD	1743:1746	arg1	degradation					1662:1672	the degradation	1658:1672	the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD	1658:1746	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	7	71	theme	polysaccharides	1681:1695	arg1	degradation					1662:1672	the degradation	1658:1672	the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD	1658:1746	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	0	72	theme	traditional	84:94	arg1	decoction					71:79	Si-Jun-Zi decoction	61:79	Si-Jun-Zi decoction	61:79	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	0	72	theme	traditional	84:94	arg1	prescription					126:137	a traditional ginseng-containing multi-herb prescription	82:137	a traditional ginseng-containing multi-herb prescription	82:137	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	8	73	from	profiles	1880:1887	arg1	SJZD					1892:1895	SJZD	1892:1895	SJZD	1892:1895	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	9	74	theme	sulfur-fumigated	1913:1928	arg1	ginseng					1930:1936	sulfur-fumigated ginseng	1913:1936	sulfur-fumigated ginseng	1913:1936	In conclusion, sulfur-fumigated ginseng altered the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs.
35816772	0	75	theme	multi-herb	115:124	arg1	decoction					71:79	Si-Jun-Zi decoction	61:79	Si-Jun-Zi decoction	61:79	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	0	75	theme	multi-herb	115:124	arg1	prescription					126:137	a traditional ginseng-containing multi-herb prescription	82:137	a traditional ginseng-containing multi-herb prescription	82:137	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	4	76	from	alteration	931:940	arg1	SJZD					972:975	SJZD	972:975	SJZD	972:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	4	77	from	derivatives	915:925	arg1	SJZD					972:975	SJZD	972:975	SJZD	972:975	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	5	78	theme	monosaccharides	1216:1230	arg1	contents					1178:1185	the contents	1174:1185	the contents of oligosaccharides and free monosaccharides of SJZD	1174:1238	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	9	79	theme	global	1950:1955	arg1	quality					1957:1963	the global quality	1946:1963	the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs	1946:2102	In conclusion, sulfur-fumigated ginseng altered the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs.
35816772	0	80	theme	ginseng	28:34	arg1	Effects					0:6	Effects	0:6	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription,	0:138	Effects of sulfur-fumigated ginseng on the global quality of Si-Jun-Zi decoction, a traditional ginseng-containing multi-herb prescription, evaluated by metabolomics and glycomics strategies.
35816772	1	81	theme	Medicine	289:296	arg1	prescription					309:320	a traditional Chinese Medicine multi-herb prescription	267:320	a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc	267:374	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	5	82	theme	total	1044:1048	arg1	contents					1050:1057	total contents	1044:1057	total contents	1044:1057	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	3	83	theme	global	673:678	arg1	quality					680:686	global quality	673:686	global quality of SJZD	673:694	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	2	84	theme	ginseng	436:442	arg1	half					413:416	nearly half	406:416	nearly half of the commercial ginseng	406:442	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	3	85	dep	metabolomics	735:746	arg1	strategies					804:813	based glycomics strategies	788:813	based glycomics strategies	788:813	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	2	86	dep	residue	550:556	arg1	transformation					583:596	transformation	583:596	transformation	583:596	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	5	87	theme	monosaccharide	1113:1126	arg1	composition					1128:1138	the monosaccharide composition	1109:1138	the monosaccharide composition of polysaccharides	1109:1157	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	7	88	theme	derivatives	1430:1440	arg1	generation					1398:1407	the generation	1394:1407	the generation of sulfur-containing derivatives	1394:1440	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	2	89	theme	processing	489:498	arg1	methods					500:506	the postharvest processing methods	473:506	the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs	473:613	Previous studies showed that nearly half of the commercial ginseng was sulfur-fumigated, one of the postharvest processing methods that commonly causes sulfur-dioxide (SO2) residue and chemical composition transformation in medical herbs.
35816772	3	90	theme	ginseng	662:668	arg1	effect					635:640	the effect	631:640	the effect of sulfur-fumigated ginseng on global quality of SJZD	631:694	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	1	91	with	decoction	202:210	arg1	ginseng					224:230	ginseng	224:230	ginseng	224:230	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
35816772	7	92	theme	flavonoids	1631:1640	arg1	dissolution					1603:1613	the dissolution	1599:1613	the dissolution of saponins and flavonoids	1599:1640	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	5	93	theme	polysaccharides	1084:1098	arg1	contents					1050:1057	total contents	1044:1057	total contents	1044:1057	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	5	93	theme	polysaccharides	1084:1098	arg1	weights					1073:1079	molecular weights	1063:1079	molecular weights	1063:1079	For saccharide components, sulfur-fumigated ginseng decreased the total contents and molecular weights of polysaccharides, changed the monosaccharide composition of polysaccharides, and increased the contents of oligosaccharides and free monosaccharides of SJZD.
35816772	9	94	theme	extra	1994:1998	arg1	attention					2000:2008	extra attention	1994:2008	extra attention	1994:2008	In conclusion, sulfur-fumigated ginseng altered the global quality of SJZD, which promoted that extra attention must be paid during the application of herbal formulas that containing sulfur-fumigated herbs.
35816772	7	95	dep	phenomena	1333:1341	arg1	attributed					1352:1361	attributed	1352:1361	might be attributed to 1) sulfur-fumigation	1343:1385	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	4	96	theme	sulfur-fumigated	848:863	arg1	ginseng					865:871	sulfur-fumigated ginseng	848:871	sulfur-fumigated ginseng	848:871	For non-saccharides components, sulfur-fumigated ginseng led to the emergence of sulfur-containing derivatives and alteration of saponins and flavonoids in SJZD.
35816772	8	97	theme	sulfur-fumigated	1806:1821	arg1	ginseng					1823:1829	sulfur-fumigated ginseng	1806:1829	sulfur-fumigated ginseng	1806:1829	Furthermore, although storage reduced the SO2 residue in sulfur-fumigated ginseng, it couldn't recover the alterations of chemical profiles in SJZD.
35816772	7	98	theme	saponins	1618:1625	arg1	dissolution					1603:1613	the dissolution	1599:1613	the dissolution of saponins and flavonoids	1599:1640	Those phenomena might be attributed to 1) sulfur-fumigation caused the generation of sulfur-containing derivatives in ginseng, which further transferred to SJZD, and 2) sulfur-fumigation caused the residue of SO2 in ginseng, which reduced the pH value and further changed the dissolution of saponins and flavonoids and accelerated the degradation of the polysaccharides to oligosaccharides and/or monosaccharides in SJZD.
35816772	3	99	theme	based	729:733	arg1	metabolomics					735:746	UPLC-QTOF-MS/MS based metabolomics	713:746	UPLC-QTOF-MS/MS based metabolomics	713:746	In this study, the effect of sulfur-fumigated ginseng on global quality of SJZD was evaluated by UPLC-QTOF-MS/MS based metabolomics and multiple chromatographic techniques based glycomics strategies.
35816772	1	100	theme	medicinal	249:257	arg1	herb					259:262	the principal medicinal herb	235:262	the principal medicinal herb	235:262	Si-Jun-Zi decoction (SJZD) with ginseng as the principal medicinal herb is a traditional Chinese Medicine multi-herb prescription that commonly employed to treat colorectal cancer etc.
36587075	8	0	theme	short	986:990	arg1	CRISPR					1013:1018	CRISPR	1013:1018	CRISPR	1013:1018	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	8	0	theme	short	986:990	arg1	repeats					1004:1010	short palindromic repeats	986:1010	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	4	1	contain	have	481:484	arg2	variety					493:499	a wide variety	486:499	a wide variety of glycans	486:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	1	contain	have	481:484	arg1	GSLs					476:479	GSLs	476:479	GSLs	476:479	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	1	contain	have	481:484	arg2	groups					520:525	head groups	515:525	head groups	515:525	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	1	contain	have	481:484	arg2	glycans					504:510	glycans	504:510	glycans	504:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	9	2	theme	sphingolipid	1239:1250	arg1	species					1252:1258	sphingolipid species	1239:1258	sphingolipid species	1239:1258	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	4	3	theme	wide	488:491	arg1	glycans					504:510	glycans	504:510	glycans	504:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	3	theme	wide	488:491	arg1	groups					520:525	head groups	515:525	head groups	515:525	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	3	theme	wide	488:491	arg1	variety					493:499	a wide variety	486:499	a wide variety of glycans	486:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	8	4	theme	repeats	1004:1010	arg1	system					1026:1031	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	9	5	theme	useful	1187:1192	arg1	tool					1194:1197	a useful tool	1185:1197	a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis	1185:1296	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	9	5	theme	useful	1187:1192	arg1	panel					1176:1180	This sphingolipid-remodeled cell panel	1143:1180	This sphingolipid-remodeled cell panel	1143:1180	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	8	6	theme	changes	1057:1063	arg1	confirmation					1041:1052	confirmation	1041:1052	confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography	1041:1140	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	8	7	theme	HeLa	933:936	arg1	cells					938:942	various sphingolipid-related gene knockout HeLa cells	890:942	various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	890:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	6	8	theme	gene	689:692	arg1	manipulation					694:705	precise gene manipulation	681:705	precise gene manipulation	681:705	To analyze the cellular function of each sphingolipid species, precise gene manipulation is essential.
36587075	2	9	theme	key	158:160	arg1	diversity					203:211	their diversity	197:211	their diversity of molecular species	197:232	The key characteristic of sphingolipids is their diversity of molecular species.
36587075	2	9	theme	key	158:160	arg1	characteristic					162:175	The key characteristic	154:175	The key characteristic of sphingolipids	154:192	The key characteristic of sphingolipids is their diversity of molecular species.
36587075	8	10	theme	lipid	1074:1078	arg1	composition					1080:1090	their lipid composition	1068:1090	their lipid composition using radioisotopes and thin layer chromatography	1068:1140	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	6	11	theme	precise	681:687	arg1	manipulation					694:705	precise gene manipulation	681:705	precise gene manipulation	681:705	To analyze the cellular function of each sphingolipid species, precise gene manipulation is essential.
36587075	5	12	theme	sphingolipid	596:607	arg1	species					609:615	sphingolipid species	596:615	sphingolipid species	596:615	Various fatty acids in ceramide also contribute to the diversity of sphingolipid species.
36587075	7	13	from	developments	728:739	arg1	technologies					759:770	genome editing technologies	744:770	genome editing technologies	744:770	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	7	14	contain	have	772:775	arg1	developments					728:739	Recent developments	721:739	Recent developments in genome editing technologies	721:770	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	7	14	contain	have	772:775	arg2	disruption					803:812	facilitated complete gene disruption	777:812	facilitated complete gene disruption	777:812	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	8	15	theme	various	890:896	arg1	cells					938:942	various sphingolipid-related gene knockout HeLa cells	890:942	various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	890:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	7	16	theme	editing	751:757	arg1	technologies					759:770	genome editing technologies	744:770	genome editing technologies	744:770	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	8	17	from	confirmation	1041:1052	arg1	composition					1080:1090	their lipid composition	1068:1090	their lipid composition using radioisotopes and thin layer chromatography	1068:1140	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	5	18	theme	species	609:615	arg1	diversity					583:591	the diversity	579:591	the diversity of sphingolipid species	579:615	Various fatty acids in ceramide also contribute to the diversity of sphingolipid species.
36587075	8	19	theme	/Cas9	1020:1024	arg1	system					1026:1031	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	6	20	theme	species	672:678	arg1	function					642:649	the cellular function	629:649	the cellular function of each sphingolipid species	629:678	To analyze the cellular function of each sphingolipid species, precise gene manipulation is essential.
36587075	8	21	theme	layer	1121:1125	arg1	chromatography					1127:1140	thin layer chromatography	1116:1140	thin layer chromatography	1116:1140	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	6	22	theme	sphingolipid	659:670	arg1	species					672:678	each sphingolipid species	654:678	each sphingolipid species	654:678	To analyze the cellular function of each sphingolipid species, precise gene manipulation is essential.
36587075	0	23	theme	Remodeled	29:37	arg1	Cells					39:43	Sphingolipid Remodeled Cells	16:43	Sphingolipid Remodeled Cells	16:43	Construction of Sphingolipid Remodeled Cells by Genome Editing.
36587075	8	24	from	changes	1057:1063	arg1	composition					1080:1090	their lipid composition	1068:1090	their lipid composition using radioisotopes and thin layer chromatography	1068:1140	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	9	25	theme	cellular	1217:1224	arg1	functions					1226:1234	the cellular functions	1213:1234	the cellular functions of sphingolipid species and as a reference for lipid analysis	1213:1296	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	8	26	theme	sphingolipid-related	898:917	arg1	cells					938:942	various sphingolipid-related gene knockout HeLa cells	890:942	various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	890:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	0	27	theme	Sphingolipid	16:27	arg1	Cells					39:43	Sphingolipid Remodeled Cells	16:43	Sphingolipid Remodeled Cells	16:43	Construction of Sphingolipid Remodeled Cells by Genome Editing.
36587075	7	28	theme	complete	789:796	arg1	disruption					803:812	facilitated complete gene disruption	777:812	facilitated complete gene disruption	777:812	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	5	29	from	acids	542:546	arg1	ceramide					551:558	ceramide	551:558	ceramide	551:558	Various fatty acids in ceramide also contribute to the diversity of sphingolipid species.
36587075	9	30	theme	sphingolipid-remodeled	1148:1169	arg1	tool					1194:1197	a useful tool	1185:1197	a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis	1185:1296	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	9	30	theme	sphingolipid-remodeled	1148:1169	arg1	panel					1176:1180	This sphingolipid-remodeled cell panel	1143:1180	This sphingolipid-remodeled cell panel	1143:1180	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	7	31	theme	cultured	817:824	arg1	cells					826:830	cultured cells	817:830	cultured cells	817:830	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	8	32	theme	gene	919:922	arg1	cells					938:942	various sphingolipid-related gene knockout HeLa cells	890:942	various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	890:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	7	33	theme	gene	798:801	arg1	disruption					803:812	facilitated complete gene disruption	777:812	facilitated complete gene disruption	777:812	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	4	34	from	species	442:448	arg1	cells					463:467	mammalian cells	453:467	mammalian cells	453:467	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	35	theme	sphingolipid	429:440	arg1	species					442:448	the most abundant sphingolipid species	411:448	the most abundant sphingolipid species in mammalian cells	411:467	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	35	theme	sphingolipid	429:440	arg1	SM					405:406	SM	405:406	SM	405:406	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	8	36	theme	knockout	924:931	arg1	cells					938:942	various sphingolipid-related gene knockout HeLa cells	890:942	various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	890:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	0	37	theme	Cells	39:43	arg1	Construction					0:11	Construction	0:11	Construction of Sphingolipid Remodeled Cells by Genome Editing.	0:62	Construction of Sphingolipid Remodeled Cells by Genome Editing.
36587075	4	38	theme	glycans	504:510	arg1	glycans					504:510	glycans	504:510	glycans	504:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	38	theme	glycans	504:510	arg1	groups					520:525	head groups	515:525	head groups	515:525	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	38	theme	glycans	504:510	arg1	variety					493:499	a wide variety	486:499	a wide variety of glycans	486:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	8	39	theme	thin	1116:1119	arg1	chromatography					1127:1140	thin layer chromatography	1116:1140	thin layer chromatography	1116:1140	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	4	40	theme	mammalian	453:461	arg1	cells					463:467	mammalian cells	453:467	mammalian cells	453:467	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	9	41	theme	cell	1171:1174	arg1	tool					1194:1197	a useful tool	1185:1197	a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis	1185:1296	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	9	41	theme	cell	1171:1174	arg1	panel					1176:1180	This sphingolipid-remodeled cell panel	1143:1180	This sphingolipid-remodeled cell panel	1143:1180	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	3	42	theme	polar	387:391	arg1	head-group					393:402	a polar head-group	385:402	a polar head-group	385:402	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	7	43	theme	facilitated	777:787	arg1	disruption					803:812	facilitated complete gene disruption	777:812	facilitated complete gene disruption	777:812	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	9	44	theme	species	1252:1258	arg1	functions					1226:1234	the cellular functions	1213:1234	the cellular functions of sphingolipid species and as a reference for lipid analysis	1213:1296	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	9	45	theme	lipid	1283:1287	arg1	analysis					1289:1296	lipid analysis	1283:1296	lipid analysis	1283:1296	This sphingolipid-remodeled cell panel is a useful tool for analyzing the cellular functions of sphingolipid species and as a reference for lipid analysis.
36587075	8	46	theme	cells	938:942	arg1	construction					874:885	the construction	870:885	the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	870:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	0	47	theme	Genome	48:53	arg1	Editing					55:61	Genome Editing	48:61	Genome Editing	48:61	Construction of Sphingolipid Remodeled Cells by Genome Editing.
36587075	4	48	theme	head	515:518	arg1	glycans					504:510	glycans	504:510	glycans	504:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	48	theme	head	515:518	arg1	groups					520:525	head groups	515:525	head groups	515:525	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	48	theme	head	515:518	arg1	variety					493:499	a wide variety	486:499	a wide variety of glycans	486:510	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	3	49	theme	sphingolipids	312:324	arg1	components					298:307	the major components	288:307	the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group	288:402	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	3	49	theme	sphingolipids	312:324	arg1	glycosphingolipids					258:275	glycosphingolipids	258:275	glycosphingolipids (GSLs)	258:282	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	3	49	theme	sphingolipids	312:324	arg1	Sphingomyelin					235:247	Sphingomyelin	235:247	Sphingomyelin (SM)	235:252	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	2	50	theme	species	226:232	arg1	diversity					203:211	their diversity	197:211	their diversity of molecular species	197:232	The key characteristic of sphingolipids is their diversity of molecular species.
36587075	2	50	theme	species	226:232	arg1	characteristic					162:175	The key characteristic	154:175	The key characteristic of sphingolipids	154:192	The key characteristic of sphingolipids is their diversity of molecular species.
36587075	5	51	theme	Various	528:534	arg1	acids					542:546	Various fatty acids	528:546	Various fatty acids in ceramide	528:558	Various fatty acids in ceramide also contribute to the diversity of sphingolipid species.
36587075	1	52	theme	various	128:134	arg1	roles					147:151	various functional roles	128:151	various functional roles	128:151	Sphingolipids are ubiquitously expressed in eukaryotes and play various functional roles.
36587075	6	53	theme	cellular	633:640	arg1	function					642:649	the cellular function	629:649	the cellular function of each sphingolipid species	629:678	To analyze the cellular function of each sphingolipid species, precise gene manipulation is essential.
36587075	3	54	theme	major	292:296	arg1	components					298:307	the major components	288:307	the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group	288:402	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	3	54	theme	major	292:296	arg1	glycosphingolipids					258:275	glycosphingolipids	258:275	glycosphingolipids (GSLs)	258:282	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	3	54	theme	major	292:296	arg1	Sphingomyelin					235:247	Sphingomyelin	235:247	Sphingomyelin (SM)	235:252	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	2	55	theme	molecular	216:224	arg1	species					226:232	molecular species	216:232	molecular species	216:232	The key characteristic of sphingolipids is their diversity of molecular species.
36587075	5	56	theme	fatty	536:540	arg1	acids					542:546	Various fatty acids	528:546	Various fatty acids in ceramide	528:558	Various fatty acids in ceramide also contribute to the diversity of sphingolipid species.
36587075	1	57	theme	functional	136:145	arg1	roles					147:151	various functional roles	128:151	various functional roles	128:151	Sphingolipids are ubiquitously expressed in eukaryotes and play various functional roles.
36587075	4	58	theme	abundant	420:427	arg1	species					442:448	the most abundant sphingolipid species	411:448	the most abundant sphingolipid species in mammalian cells	411:467	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	4	58	theme	abundant	420:427	arg1	SM					405:406	SM	405:406	SM	405:406	SM is the most abundant sphingolipid species in mammalian cells, while GSLs have a wide variety of glycans as head groups.
36587075	8	59	theme	interspaced	974:984	arg1	CRISPR					1013:1018	CRISPR	1013:1018	CRISPR	1013:1018	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	8	59	theme	interspaced	974:984	arg1	repeats					1004:1010	short palindromic repeats	986:1010	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	3	60	from	components	298:307	arg1	membrane					340:347	the plasma membrane	329:347	the plasma membrane	329:347	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	3	61	theme	plasma	333:338	arg1	membrane					340:347	the plasma membrane	329:347	the plasma membrane	329:347	Sphingomyelin (SM) and glycosphingolipids (GSLs) are the major components of sphingolipids in the plasma membrane, which are composed of ceramide and a polar head-group.
36587075	7	62	theme	genome	744:749	arg1	technologies					759:770	genome editing technologies	744:770	genome editing technologies	744:770	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	2	63	theme	sphingolipids	180:192	arg1	diversity					203:211	their diversity	197:211	their diversity of molecular species	197:232	The key characteristic of sphingolipids is their diversity of molecular species.
36587075	2	63	theme	sphingolipids	180:192	arg1	characteristic					162:175	The key characteristic	154:175	The key characteristic of sphingolipids	154:192	The key characteristic of sphingolipids is their diversity of molecular species.
36587075	7	64	theme	Recent	721:726	arg1	developments					728:739	Recent developments	721:739	Recent developments in genome editing technologies	721:770	Recent developments in genome editing technologies have facilitated complete gene disruption in cultured cells.
36587075	8	65	theme	palindromic	992:1002	arg1	CRISPR					1013:1018	CRISPR	1013:1018	CRISPR	1013:1018	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	8	65	theme	palindromic	992:1002	arg1	repeats					1004:1010	short palindromic repeats	986:1010	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	8	66	theme	clustered	954:962	arg1	system					1026:1031	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system	950:1031	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36587075	8	67	from	composition	1080:1090	arg1	confirmation					1041:1052	confirmation	1041:1052	confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography	1041:1140	This chapter describes protocols for the construction of various sphingolipid-related gene knockout HeLa cells using the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system and for confirmation of changes in their lipid composition using radioisotopes and thin layer chromatography.
36262138	0	0	theme	machine	80:86	arg1	learning					88:95	machine learning	80:95	machine learning model	80:101	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	0	1	from	study	14:18	arg1	identification					27:40	the identification	23:40	the identification of N-linked glycosylation sites	23:72	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	6	2	theme	research	738:745	arg1	articles					747:754	Almost seventy research articles	723:754	Almost seventy research articles published in recognized journals of the N-linked glycosylation field	723:823	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	1	3	theme	glycosylation	140:152	arg1	type					132:135	the most common type	116:135	the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development	116:280	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	1	3	theme	glycosylation	140:152	arg1	N-linked					104:111	N-linked	104:111	N-linked	104:111	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	4	4	theme	N-linked	590:597	arg1	places					599:604	N-linked places	590:604	N-linked places using machine learning methods	590:635	This study aims to analyze and synthesize the progress to discover N-linked places using machine learning methods.
36262138	6	5	theme	rigorous	852:859	arg1	process					871:877	the rigorous filtering process	848:877	the rigorous filtering process	848:877	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	2	6	theme	biological	325:334	arg1	functionalities					354:368	their biological and psychological functionalities	319:368	their biological and psychological functionalities	319:368	Most of the proteins cannot perform their biological and psychological functionalities without undergoing such modification.
36262138	7	7	dep	aspects	945:951	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	7	dep	aspects	945:951	arg1	evaluation					1044:1053	performance evaluation	1032:1053	performance evaluation	1032:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	7	dep	aspects	945:951	arg1	method					1000:1005	publication channel, feature set construction method	954:1005	method	1000:1005	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	7	dep	aspects	945:951	arg1	algorithm					1017:1025	training algorithm	1008:1025	training algorithm	1008:1025	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	0	8	gly	glycosylation	54:66	arg2	sites					68:72	N-linked glycosylation sites	45:72	N-linked glycosylation sites	45:72	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	0	9	theme	learning	88:95	arg1	model					97:101	machine learning model	80:101	machine learning model	80:101	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	5	10	theme	available	684:692	arg1	tools					694:698	currently available tools	674:698	currently available tools	674:698	It also explores the performance of currently available tools to predict such sites.
36262138	7	11	theme	set	983:985	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	11	theme	set	983:985	arg1	method					1000:1005	publication channel, feature set construction method	954:1005	method	1000:1005	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	6	12	theme	seventy	730:736	arg1	articles					747:754	Almost seventy research articles	723:754	Almost seventy research articles published in recognized journals of the N-linked glycosylation field	723:823	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	7	13	theme	construction	987:998	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	13	theme	construction	987:998	arg1	method					1000:1005	publication channel, feature set construction method	954:1005	method	1000:1005	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	14	theme	studies	900:906	arg1	findings					884:891	The findings	880:891	The findings of the studies	880:906	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	9	15	link	N-linked	1228:1235	arg1	glycosylation					1237:1249	N-linked glycosylation	1228:1249	N-linked glycosylation	1228:1249	Our study focuses on the performance evaluation criteria, and the importance of N-linked glycosylation motivates us to discover resources that use computational methods instead of the experimental method due to its limitations.
36262138	0	16	link	N-linked	45:52	arg1	sites					68:72	N-linked glycosylation sites	45:72	N-linked glycosylation sites	45:72	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	8	17	link	N-linked	1114:1121	arg1	identification					1132:1145	N-linked sequence identification	1114:1145	N-linked sequence identification	1114:1145	Moreover, a literature survey has developed a taxonomy of N-linked sequence identification.
36262138	2	18	theme	such	389:392	arg1	modification					394:405	such modification	389:405	such modification	389:405	Most of the proteins cannot perform their biological and psychological functionalities without undergoing such modification.
36262138	0	19	theme	analytical	3:12	arg1	study					14:18	An analytical study	0:18	An analytical study on the identification of N-linked glycosylation sites	0:72	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	8	20	theme	literature	1068:1077	arg1	survey					1079:1084	a literature survey	1066:1084	a literature survey	1066:1084	Moreover, a literature survey has developed a taxonomy of N-linked sequence identification.
36262138	6	21	theme	field	819:823	arg1	journals					780:787	recognized journals	769:787	recognized journals of the N-linked glycosylation field	769:823	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	1	22	theme	significant	168:178	arg1	role					180:183	a significant role	166:183	a significant role	166:183	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	6	23	theme	glycosylation	805:817	arg1	field					819:823	the N-linked glycosylation field	792:823	the N-linked glycosylation field	792:823	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	7	24	theme	publication	954:964	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	24	theme	publication	954:964	arg1	method					1000:1005	publication channel, feature set construction method	954:1005	method	1000:1005	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	8	25	theme	N-linked	1114:1121	arg1	identification					1132:1145	N-linked sequence identification	1114:1145	N-linked sequence identification	1114:1145	Moreover, a literature survey has developed a taxonomy of N-linked sequence identification.
36262138	6	26	theme	N-linked	796:803	arg1	field					819:823	the N-linked glycosylation field	792:823	the N-linked glycosylation field	792:823	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	1	27	theme	drug	265:268	arg1	development					270:280	drug development	265:280	drug development	265:280	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	9	28	theme	performance	1173:1183	arg1	criteria					1196:1203	the performance evaluation criteria	1169:1203	the performance evaluation criteria	1169:1203	Our study focuses on the performance evaluation criteria, and the importance of N-linked glycosylation motivates us to discover resources that use computational methods instead of the experimental method due to its limitations.
36262138	9	29	theme	N-linked	1228:1235	arg1	glycosylation					1237:1249	N-linked glycosylation	1228:1249	N-linked glycosylation	1228:1249	Our study focuses on the performance evaluation criteria, and the importance of N-linked glycosylation motivates us to discover resources that use computational methods instead of the experimental method due to its limitations.
36262138	3	30	theme	computational	461:473	arg1	techniques					475:484	computational techniques	461:484	computational techniques	461:484	Therefore, it is essential to identify such sites by computational techniques because of experimental limitations.
36262138	8	31	theme	identification	1132:1145	arg1	taxonomy					1102:1109	a taxonomy	1100:1109	a taxonomy of N-linked sequence identification	1100:1145	Moreover, a literature survey has developed a taxonomy of N-linked sequence identification.
36262138	1	32	theme	various	200:206	arg1	cancer					245:250	cancer	245:250	cancer	245:250	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	1	32	theme	various	200:206	arg1	diabetes					232:239	type I diabetes	225:239	type I diabetes	225:239	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	1	32	theme	various	200:206	arg1	diseases					208:215	various diseases	200:215	various diseases such as type I diabetes and cancer	200:250	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	3	33	theme	such	447:450	arg1	sites					452:456	such sites	447:456	such sites	447:456	Therefore, it is essential to identify such sites by computational techniques because of experimental limitations.
36262138	8	34	theme	sequence	1123:1130	arg1	identification					1132:1145	N-linked sequence identification	1114:1145	N-linked sequence identification	1114:1145	Moreover, a literature survey has developed a taxonomy of N-linked sequence identification.
36262138	9	35	theme	glycosylation	1237:1249	arg1	importance					1214:1223	the importance	1210:1223	the importance of N-linked glycosylation	1210:1249	Our study focuses on the performance evaluation criteria, and the importance of N-linked glycosylation motivates us to discover resources that use computational methods instead of the experimental method due to its limitations.
36262138	0	36	theme	N-linked	45:52	arg1	sites					68:72	N-linked glycosylation sites	45:72	N-linked glycosylation sites	45:72	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	5	37	theme	tools	694:698	arg1	performance					659:669	the performance	655:669	the performance of currently available tools to predict such sites	655:720	It also explores the performance of currently available tools to predict such sites.
36262138	9	38	theme	computational	1295:1307	arg1	methods					1309:1315	computational methods	1295:1315	computational methods	1295:1315	Our study focuses on the performance evaluation criteria, and the importance of N-linked glycosylation motivates us to discover resources that use computational methods instead of the experimental method due to its limitations.
36262138	9	39	theme	evaluation	1185:1194	arg1	criteria					1196:1203	the performance evaluation criteria	1169:1203	the performance evaluation criteria	1169:1203	Our study focuses on the performance evaluation criteria, and the importance of N-linked glycosylation motivates us to discover resources that use computational methods instead of the experimental method due to its limitations.
36262138	7	40	theme	performance	1032:1042	arg1	evaluation					1044:1053	performance evaluation	1032:1053	performance evaluation	1032:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	40	theme	performance	1032:1042	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	3	41	theme	experimental	497:508	arg1	limitations					510:520	experimental limitations	497:520	experimental limitations	497:520	Therefore, it is essential to identify such sites by computational techniques because of experimental limitations.
36262138	7	42	theme	channel	966:972	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	42	theme	channel	966:972	arg1	method					1000:1005	publication channel, feature set construction method	954:1005	method	1000:1005	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	6	43	theme	recognized	769:778	arg1	journals					780:787	recognized journals	769:787	recognized journals of the N-linked glycosylation field	769:823	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	4	44	theme	learning	620:627	arg1	methods					629:635	machine learning methods	612:635	machine learning methods	612:635	This study aims to analyze and synthesize the progress to discover N-linked places using machine learning methods.
36262138	5	45	theme	such	711:714	arg1	sites					716:720	such sites	711:720	such sites	711:720	It also explores the performance of currently available tools to predict such sites.
36262138	4	46	link	N-linked	590:597	arg1	places					599:604	N-linked places	590:604	N-linked places using machine learning methods	590:635	This study aims to analyze and synthesize the progress to discover N-linked places using machine learning methods.
36262138	1	47	theme	common	125:130	arg1	type					132:135	the most common type	116:135	the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development	116:280	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	1	47	theme	common	125:130	arg1	N-linked					104:111	N-linked	104:111	N-linked	104:111	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	7	48	dep	method	1000:1005	arg1	feature					975:981	publication channel, feature set construction method	954:1005	feature	975:981	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	4	49	theme	machine	612:618	arg1	learning					620:627	machine learning	612:627	machine learning methods	612:635	This study aims to analyze and synthesize the progress to discover N-linked places using machine learning methods.
36262138	0	50	theme	sites	68:72	arg1	identification					27:40	the identification	23:40	the identification of N-linked glycosylation sites	23:72	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	6	51	theme	filtering	861:869	arg1	process					871:877	the rigorous filtering process	848:877	the rigorous filtering process	848:877	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36262138	7	52	theme	multiple	936:943	arg1	method					1000:1005	publication channel, feature set construction method	954:1005	method	1000:1005	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	52	theme	multiple	936:943	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	52	theme	multiple	936:943	arg1	algorithm					1017:1025	training algorithm	1008:1025	training algorithm	1008:1025	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	52	theme	multiple	936:943	arg1	evaluation					1044:1053	performance evaluation	1032:1053	performance evaluation	1032:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	1	53	theme	type	225:228	arg1	diabetes					232:239	type I diabetes	225:239	type I diabetes	225:239	N-linked is the most common type of glycosylation which plays a significant role in identifying various diseases such as type I diabetes and cancer and helps in drug development.
36262138	0	54	theme	glycosylation	54:66	arg1	sites					68:72	N-linked glycosylation sites	45:72	N-linked glycosylation sites	45:72	An analytical study on the identification of N-linked glycosylation sites using machine learning model.
36262138	9	55	theme	experimental	1332:1343	arg1	method					1345:1350	the experimental method	1328:1350	the experimental method	1328:1350	Our study focuses on the performance evaluation criteria, and the importance of N-linked glycosylation motivates us to discover resources that use computational methods instead of the experimental method due to its limitations.
36262138	7	56	theme	training	1008:1015	arg1	aspects					945:951	multiple aspects	936:951	multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation	936:1053	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	7	56	theme	training	1008:1015	arg1	algorithm					1017:1025	training algorithm	1008:1025	training algorithm	1008:1025	The findings of the studies have been reported based on multiple aspects: publication channel, feature set construction method, training algorithm, and performance evaluation.
36262138	2	57	theme	psychological	340:352	arg1	functionalities					354:368	their biological and psychological functionalities	319:368	their biological and psychological functionalities	319:368	Most of the proteins cannot perform their biological and psychological functionalities without undergoing such modification.
36262138	6	58	link	N-linked	796:803	arg1	field					819:823	the N-linked glycosylation field	792:823	the N-linked glycosylation field	792:823	Almost seventy research articles published in recognized journals of the N-linked glycosylation field have shortlisted after the rigorous filtering process.
36911039	0	0	theme	growth	94:99	arg1	deficiency					109:118	growth hormone deficiency	94:118	growth hormone deficiency	94:118	Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.
36911039	9	1	theme	components	1539:1548	arg1	abundance					1515:1523	The abundance	1511:1523	The abundance of various GM components	1511:1548	The abundance of various GM components was correlated with endocrine hormone levels.
36911039	10	2	theme	endocrine	1752:1760	arg1	hormones					1762:1769	endocrine hormones	1752:1769	endocrine hormones	1752:1769	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	3	3	theme	Total	501:505	arg1	DNA					507:509	Total DNA	501:509	Total DNA	501:509	Total DNA was extracted and amplified from stool samples obtained from all subjects.
36911039	6	4	theme	Bacteroidetes	838:850	arg1	higher					870:875	higher	870:875	higher	870:875	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	4	theme	Bacteroidetes	838:850	arg1	abundance					814:822	the abundance	810:822	the abundance of the phylum Bacteroidetes	810:850	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	7	5	from	abundance	993:1001	arg1	group					1032:1036	the disease group	1020:1036	the disease group	1020:1036	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	1	6	from	functions	171:179	arg1	children					211:218	children	211:218	children with growth hormone deficiency (GHD) using high-throughput sequencing	211:288	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	2	7	dep	Maternity	361:369	arg1	Hospital					388:395	Hospital	388:395	Hospital	388:395	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	7	8	dep	lower	1161:1165	arg1	%					1173:1173	6.576%	1168:1173	6.576%	1168:1173	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	7	8	dep	lower	1161:1165	arg1	%					1183:1183	1.75%	1179:1183	1.75%	1179:1183	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	4	9	theme	High-throughput	586:600	arg1	sequencing					602:611	High-throughput sequencing	586:611	High-throughput sequencing	586:611	High-throughput sequencing was used to analyze the GM composition and functions.
36911039	0	10	theme	hormone	101:107	arg1	deficiency					109:118	growth hormone deficiency	94:118	growth hormone deficiency	94:118	Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.
36911039	0	11	with	children	80:87	arg1	deficiency					109:118	growth hormone deficiency	94:118	growth hormone deficiency	94:118	Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.
36911039	2	12	with	children	312:319	arg1	GHD					326:328	GHD	326:328	GHD	326:328	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	7	13	theme	disease	1024:1030	arg1	group					1032:1036	the disease group	1020:1036	the disease group	1020:1036	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	1	14	from	composition	155:165	arg1	children					211:218	children	211:218	children with growth hormone deficiency (GHD) using high-throughput sequencing	211:288	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	7	15	dep	%	1173:1173	arg1	%					1207:1207	5.08%	1203:1207	5.08%	1203:1207	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	7	15	dep	%	1173:1173	arg1	%					1217:1217	2.02%	1213:1217	2.02%	1213:1217	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	7	15	dep	%	1173:1173	arg1	%					1190:1190	4.51%	1186:1190	4.51%	1186:1190	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	7	15	dep	%	1173:1173	arg1	%					1200:1200	0.80%	1196:1200	0.80%	1196:1200	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	5	16	from	groups	695:700	arg1	GM					679:680	The GM	675:680	The GM from the two groups of children	675:712	Results The GM from the two groups of children showed significant differences in α-diversity (P < 0.05).
36911039	9	17	theme	hormone	1580:1586	arg1	levels					1588:1593	endocrine hormone levels	1570:1593	endocrine hormone levels	1570:1593	The abundance of various GM components was correlated with endocrine hormone levels.
36911039	1	18	from	changes	140:146	arg1	composition					155:165	composition	155:165	composition	155:165	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	1	18	from	changes	140:146	arg1	functions					171:179	functions	171:179	functions	171:179	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	0	19	theme	Gut	0:2	arg1	composition					15:25	Gut microbiota composition	0:25	Gut microbiota composition	0:25	Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.
36911039	0	20	theme	microbiota	4:13	arg1	composition					15:25	Gut microbiota composition	0:25	Gut microbiota composition	0:25	Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.
36911039	1	21	theme	gut	188:190	arg1	GM					204:205	GM	204:205	GM	204:205	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	1	21	theme	gut	188:190	arg1	microbiota					192:201	the gut microbiota	184:201	the gut microbiota (GM)	184:206	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	2	22	theme	healthy	439:445	arg1	children					447:454	24 healthy children	436:454	24 healthy children of the same age	436:470	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	9	23	theme	endocrine	1570:1578	arg1	levels					1588:1593	endocrine hormone levels	1570:1593	endocrine hormone levels	1570:1593	The abundance of various GM components was correlated with endocrine hormone levels.
36911039	1	24	theme	microbiota	192:201	arg1	composition					155:165	composition	155:165	composition	155:165	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	1	24	theme	microbiota	192:201	arg1	functions					171:179	functions	171:179	functions	171:179	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	7	25	dep	higher	1056:1061	arg1	%					1068:1068	3.16%	1064:1068	3.16%	1064:1068	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	7	25	dep	higher	1056:1061	arg1	%					1079:1079	20.67%	1074:1079	20.67%	1074:1079	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	1	26	theme	high-throughput	263:277	arg1	sequencing					279:288	high-throughput sequencing	263:288	high-throughput sequencing	263:288	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	4	27	used	used	617:620	arg2	sequencing					602:611	High-throughput sequencing	586:611	High-throughput sequencing	586:611	High-throughput sequencing was used to analyze the GM composition and functions.
36911039	10	28	from	alterations	1619:1629	arg1	GM					1638:1639	the GM	1634:1639	the GM	1634:1639	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	2	29	theme	Thirty-three	299:310	arg1	children					312:319	Thirty-three children	299:319	Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital	299:395	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	7	30	theme	Prevotella	1006:1015	arg1	abundance					993:1001	the abundance	989:1001	the abundance of Prevotella in the disease group	989:1036	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	7	30	theme	Prevotella	1006:1015	arg1	higher					1056:1061	higher	1056:1061	higher	1056:1061	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	5	31	from	differences	733:743	arg1	P < 0.05					761:768	P < 0.05	761:768	P < 0.05	761:768	Results The GM from the two groups of children showed significant differences in α-diversity (P < 0.05).
36911039	5	31	from	differences	733:743	arg1	α-diversity					748:758	α-diversity	748:758	α-diversity (P < 0.05)	748:769	Results The GM from the two groups of children showed significant differences in α-diversity (P < 0.05).
36911039	1	32	dep	composition	155:165	arg1	the					151:153	the	151:153	the	151:153	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	2	33	theme	disease	418:424	arg1	age					468:470	the same age	459:470	the same age	459:470	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	2	33	theme	disease	418:424	arg1	group					426:430	the disease group	414:430	the disease group	414:430	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	2	34	theme	age	468:470	arg1	age					468:470	the same age	459:470	the same age	459:470	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	2	34	theme	age	468:470	arg1	children					447:454	24 healthy children	436:454	24 healthy children of the same age	436:470	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	2	34	theme	age	468:470	arg1	group					426:430	the disease group	414:430	the disease group	414:430	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	10	35	theme	hormones	1762:1769	arg1	metabolism					1723:1732	energy metabolism	1716:1732	energy metabolism	1716:1732	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	10	35	theme	hormones	1762:1769	arg1	levels					1742:1747	the levels	1738:1747	the levels of endocrine hormones	1738:1769	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	10	36	theme	energy	1716:1721	arg1	metabolism					1723:1732	energy metabolism	1716:1732	energy metabolism	1716:1732	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	2	37	theme	same	463:466	arg1	age					468:470	the same age	459:470	the same age	459:470	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	5	38	theme	children	705:712	arg1	groups					695:700	the two groups	687:700	the two groups of children	687:712	Results The GM from the two groups of children showed significant differences in α-diversity (P < 0.05).
36911039	5	38	theme	children	705:712	arg1	children					705:712	children	705:712	children	705:712	Results The GM from the two groups of children showed significant differences in α-diversity (P < 0.05).
36911039	5	39	theme	significant	721:731	arg1	differences					733:743	significant differences	721:743	significant differences in α-diversity (P < 0.05)	721:769	Results The GM from the two groups of children showed significant differences in α-diversity (P < 0.05).
36911039	5	40	dep	Results	667:673	arg1	showed					714:719	showed	714:719	showed significant differences in α-diversity (P < 0.05)	714:769	Results The GM from the two groups of children showed significant differences in α-diversity (P < 0.05).
36911039	4	41	theme	GM	637:638	arg1	composition					640:650	the GM composition	633:650	the GM composition	633:650	High-throughput sequencing was used to analyze the GM composition and functions.
36911039	6	42	theme	Firmicutes	907:916	arg1	count					918:922	the Firmicutes count	903:922	the Firmicutes count	903:922	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	42	theme	Firmicutes	907:916	arg1	lower					942:946	lower	942:946	lower	942:946	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	43	theme	control	795:801	arg1	group					803:807	the control group	791:807	the control group	791:807	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	44	theme	phylum	831:836	arg1	Bacteroidetes					838:850	the phylum Bacteroidetes	827:850	the phylum Bacteroidetes	827:850	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	0	45	theme	functional	51:60	arg1	categorization					62:75	functional categorization	51:75	functional categorization	51:75	Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.
36911039	7	46	dep	Lachnospiracea	1094:1107	arg1	sedis					1118:1122	Lachnospiracea incertae sedis	1094:1122	Lachnospiracea incertae sedis	1094:1122	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	10	47	theme	growth	1795:1800	arg1	restriction					1802:1812	growth restriction	1795:1812	growth restriction	1795:1812	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	8	48	theme	GM	1235:1236	arg1	functions					1238:1246	GM functions	1235:1246	GM functions	1235:1246	GM functions, including those involved in membrane_transport, energy_metabolism, poorly_characterized, metabolism_of_cofactors_and_vitamins, glycan_biosynthesis_and_metabolism, transcription, folding,_sorting,_and_degradation, were significantly altered in the disease group.
36911039	8	48	theme	GM	1235:1236	arg1	those					1259:1263	those	1259:1263	those	1259:1263	GM functions, including those involved in membrane_transport, energy_metabolism, poorly_characterized, metabolism_of_cofactors_and_vitamins, glycan_biosynthesis_and_metabolism, transcription, folding,_sorting,_and_degradation, were significantly altered in the disease group.
36911039	2	49	dep	Methods	291:297	arg1	included					402:409	included	402:409	were included in the disease group and 24 healthy children of the same age	397:470	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	10	50	theme	hormone	1674:1680	arg1	deficiency					1682:1691	growth hormone deficiency	1667:1691	growth hormone deficiency	1667:1691	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	9	51	theme	GM	1536:1537	arg1	components					1539:1548	various GM components	1528:1548	various GM components	1528:1548	The abundance of various GM components was correlated with endocrine hormone levels.
36911039	10	52	theme	Significant	1607:1617	arg1	alterations					1619:1629	Conclusion Significant alterations	1596:1629	Conclusion Significant alterations in the GM	1596:1639	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	3	53	theme	stool	544:548	arg1	samples					550:556	stool samples	544:556	stool samples obtained from all subjects	544:583	Total DNA was extracted and amplified from stool samples obtained from all subjects.
36911039	10	54	with	children	1653:1660	arg1	deficiency					1682:1691	growth hormone deficiency	1667:1691	growth hormone deficiency	1667:1691	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	7	55	theme	genus	976:980	arg1	level					982:986	the genus level	972:986	the genus level	972:986	At the genus level, the abundance of Prevotella in the disease group was significantly higher (3.16% vs. 20.67%) and that of Lachnospiracea incertae sedis, Clostridium XlVa, and Megamonas was lower (6.576% vs. 1.75%; 4.51% vs. 0.80%; 5.08% vs. 2.02%, respectively).
36911039	10	56	theme	growth	1667:1672	arg1	deficiency					1682:1691	growth hormone deficiency	1667:1691	growth hormone deficiency	1667:1691	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	10	57	theme	Conclusion	1596:1605	arg1	alterations					1619:1629	Conclusion Significant alterations	1596:1629	Conclusion Significant alterations in the GM	1596:1639	Conclusion Significant alterations in the GM are seen in children with growth hormone deficiency, which may affect both energy metabolism and the levels of endocrine hormones, potentially leading to growth restriction.
36911039	6	58	dep	higher	870:875	arg1	%					883:883	45.96%	878:883	45.96%	878:883	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	58	dep	higher	870:875	arg1	%					894:894	65.71%	889:894	65.71%	889:894	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	59	with	comparison	775:784	arg1	group					803:807	the control group	791:807	the control group	791:807	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	60	dep	lower	942:946	arg1	%					954:954	47.09%	949:954	47.09%	949:954	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	6	60	dep	lower	942:946	arg1	%					965:965	25.20%	960:965	25.20%	960:965	In comparison with the control group, the abundance of the phylum Bacteroidetes was significantly higher (45.96% vs. 65.71%) while the Firmicutes count was significantly lower (47.09% vs. 25.20%).
36911039	9	61	theme	various	1528:1534	arg1	components					1539:1548	various GM components	1528:1548	various GM components	1528:1548	The abundance of various GM components was correlated with endocrine hormone levels.
36911039	2	62	theme	Child	375:379	arg1	Health					381:386	Child Health	375:386	Child Health	375:386	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	1	63	theme	growth	225:230	arg1	GHD					252:254	GHD	252:254	GHD	252:254	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	1	63	theme	growth	225:230	arg1	deficiency					240:249	growth hormone deficiency	225:249	growth hormone deficiency (GHD) using high-throughput sequencing	225:288	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	2	64	theme	control	486:492	arg1	group					494:498	the control group	482:498	the control group	482:498	Methods Thirty-three children with GHD diagnosed in Longgang District Maternity and Child Health Hospital were included in the disease group and 24 healthy children of the same age comprised the control group.
36911039	0	65	from	alteration	27:36	arg1	children					80:87	children	80:87	children with growth hormone deficiency	80:118	Gut microbiota composition alteration analysis and functional categorization in children with growth hormone deficiency.
36911039	8	66	theme	disease	1496:1502	arg1	group					1504:1508	the disease group	1492:1508	the disease group	1492:1508	GM functions, including those involved in membrane_transport, energy_metabolism, poorly_characterized, metabolism_of_cofactors_and_vitamins, glycan_biosynthesis_and_metabolism, transcription, folding,_sorting,_and_degradation, were significantly altered in the disease group.
36911039	1	67	with	children	211:218	arg1	GHD					252:254	GHD	252:254	GHD	252:254	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	1	67	with	children	211:218	arg1	deficiency					240:249	growth hormone deficiency	225:249	growth hormone deficiency (GHD) using high-throughput sequencing	225:288	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	1	68	theme	hormone	232:238	arg1	GHD					252:254	GHD	252:254	GHD	252:254	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
36911039	1	68	theme	hormone	232:238	arg1	deficiency					240:249	growth hormone deficiency	225:249	growth hormone deficiency (GHD) using high-throughput sequencing	225:288	Objective To study changes in the composition and functions of the gut microbiota (GM) in children with growth hormone deficiency (GHD) using high-throughput sequencing.
35140266	0	0	theme	type	74:77	arg1	C1					79:80	Niemann-Pick disease type C1	53:80	Niemann-Pick disease type C1	53:80	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	0	1	from	age	15:17	arg1	C1					79:80	Niemann-Pick disease type C1	53:80	Niemann-Pick disease type C1	53:80	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	4	2	theme	severity	811:818	arg1	age					786:788	age	786:788	age of onset and disease severity	786:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	5	3	theme	neurological	1056:1067	arg1	onset					1077:1081	particular neurological symptom onset	1045:1081	particular neurological symptom onset	1045:1081	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	1	4	theme	type	153:156	arg1	C1					158:159	type C1	153:159	Niemann-Pick disease type C1 (NPC1)	132:166	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	0	5	theme	disease	66:72	arg1	C1					79:80	Niemann-Pick disease type C1	53:80	Niemann-Pick disease type C1	53:80	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	2	6	dep	diagnosis	422:430	arg1	prompt					415:420	prompt	415:420	prompt	415:420	This heterogeneity has made it difficult to obtain prompt diagnosis and to predict disease course.
35140266	4	7	theme	NPC1	647:650	arg1	patients					652:659	41 NPC1 patients	644:659	41 NPC1 patients	644:659	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	1	8	theme	onset	321:325	arg1	severity					255:262	highly variable severity	239:262	highly variable severity	239:262	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	8	theme	onset	321:325	arg1	progression					276:286	disease progression	268:286	disease progression	268:286	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	8	theme	onset	321:325	arg1	age					314:316	a wide-ranging age	299:316	highly variable severity and disease progression as well as a wide-ranging age of onset	239:325	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	5	9	theme	genes	961:965	arg1	set					954:956	a set	952:956	a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset	952:1081	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	5	9	theme	genes	961:965	arg1	genes					961:965	genes	961:965	genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset	961:1081	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	3	10	theme	small	476:480	arg1	sizes					502:506	small NPC1 patient sample sizes	476:506	small NPC1 patient sample sizes	476:506	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	3	10	theme	small	476:480	arg1	factor					529:534	a limiting factor	518:534	a limiting factor	518:534	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	4	11	theme	lysosomal	714:722	arg1	LysoTracker					743:753	the lysosomal quantitative probe LysoTracker	710:753	the lysosomal quantitative probe LysoTracker	710:753	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	4	11	theme	lysosomal	714:722	arg1	predictor					772:780	a predictor	770:780	a predictor for age of onset and disease severity	770:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	4	12	theme	previous	687:694	arg1	findings					696:703	our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity	683:818	our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity	683:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	7	13	theme	disease	1435:1441	arg1	modifiers					1443:1451	disease modifiers	1435:1451	disease modifiers	1435:1451	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	5	14	theme	primary	917:923	arg1	fibroblasts					925:935	primary fibroblasts	917:935	primary fibroblasts	917:935	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	4	15	theme	patients	652:659	arg1	cohort					634:639	an extensive cohort	621:639	an extensive cohort of 41 NPC1 patients	621:659	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	4	16	from	cohort	634:639	arg1	fibroblasts					604:614	primary fibroblasts	596:614	primary fibroblasts from an extensive cohort of 41 NPC1 patients	596:659	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	1	17	theme	variable	246:253	arg1	severity					255:262	highly variable severity	239:262	highly variable severity	239:262	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	4	18	used	used	666:669	arg2	fibroblasts					604:614	primary fibroblasts	596:614	primary fibroblasts from an extensive cohort of 41 NPC1 patients	596:659	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	3	19	theme	patient	487:493	arg1	sizes					502:506	small NPC1 patient sample sizes	476:506	small NPC1 patient sample sizes	476:506	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	3	19	theme	patient	487:493	arg1	factor					529:534	a limiting factor	518:534	a limiting factor	518:534	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	6	20	theme	patient	1177:1183	arg1	subgroups					1185:1193	patient subgroups	1177:1193	patient subgroups	1177:1193	Hierarchical clustering showed that these genes exhibited distinct expression patterns among patient subgroups.
35140266	0	21	theme	lysosomal	87:95	arg1	abnormalities					97:109	lysosomal abnormalities	87:109	lysosomal abnormalities	87:109	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	1	22	theme	perinatal	333:341	arg1	stages					343:348	perinatal stages	333:348	perinatal stages to adulthood	333:361	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	2	23	dep	difficult	395:403	arg1	obtain					408:413	obtain	408:413	to obtain prompt diagnosis	405:430	This heterogeneity has made it difficult to obtain prompt diagnosis and to predict disease course.
35140266	2	23	dep	difficult	395:403	arg1	predict					439:445	predict	439:445	to predict disease course	436:460	This heterogeneity has made it difficult to obtain prompt diagnosis and to predict disease course.
35140266	3	24	theme	sample	495:500	arg1	sizes					502:506	small NPC1 patient sample sizes	476:506	small NPC1 patient sample sizes	476:506	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	3	24	theme	sample	495:500	arg1	factor					529:534	a limiting factor	518:534	a limiting factor	518:534	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	7	25	theme	NPC1	1372:1375	arg1	heterogeneity					1386:1398	NPC1 clinical heterogeneity	1372:1398	NPC1 clinical heterogeneity	1372:1398	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	2	26	theme	disease	447:453	arg1	course					455:460	disease course	447:460	disease course	447:460	This heterogeneity has made it difficult to obtain prompt diagnosis and to predict disease course.
35140266	7	27	theme	therapeutic	1457:1467	arg1	targets					1469:1475	therapeutic targets	1457:1475	therapeutic targets	1457:1475	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	7	28	from	data	1246:1249	arg1	scale					1267:1271	such a large scale	1254:1271	such a large scale	1254:1271	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	3	29	theme	limiting	520:527	arg1	sizes					502:506	small NPC1 patient sample sizes	476:506	small NPC1 patient sample sizes	476:506	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	3	29	theme	limiting	520:527	arg1	factor					529:534	a limiting factor	518:534	a limiting factor	518:534	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	4	30	theme	onset	793:797	arg1	age					786:788	age	786:788	age of onset and disease severity	786:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	5	31	theme	RNA	892:894	arg1	data					907:910	RNA expression data	892:910	RNA expression data	892:910	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	0	32	theme	clinical	32:39	arg1	severity					41:48	clinical severity	32:48	clinical severity	32:48	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	4	33	theme	disease	803:809	arg1	severity					811:818	disease severity	803:818	disease severity	803:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	7	34	theme	clinical	1377:1384	arg1	heterogeneity					1386:1398	NPC1 clinical heterogeneity	1372:1398	NPC1 clinical heterogeneity	1372:1398	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	0	35	theme	gene	115:118	arg1	expression					120:129	gene expression	115:129	gene expression	115:129	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	5	36	theme	expression	896:905	arg1	data					907:910	RNA expression data	892:910	RNA expression data	892:910	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	4	37	used	used	762:765	arg2	LysoTracker					743:753	the lysosomal quantitative probe LysoTracker	710:753	the lysosomal quantitative probe LysoTracker	710:753	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	4	37	used	used	762:765	arg2	predictor					772:780	a predictor	770:780	a predictor for age of onset and disease severity	770:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	0	38	theme	age	15:17	arg1	Correlation					0:10	Correlation	0:10	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.	0:130	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	1	39	theme	disease	268:274	arg1	progression					276:286	disease progression	268:286	disease progression	268:286	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	4	40	theme	probe	737:741	arg1	LysoTracker					743:753	the lysosomal quantitative probe LysoTracker	710:753	the lysosomal quantitative probe LysoTracker	710:753	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	4	40	theme	probe	737:741	arg1	predictor					772:780	a predictor	770:780	a predictor for age of onset and disease severity	770:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	1	41	dep	disease	145:151	arg1	C1					158:159	type C1	153:159	Niemann-Pick disease type C1 (NPC1)	132:166	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	42	theme	rare	173:176	arg1	disease					145:151	Niemann-Pick disease	132:151	Niemann-Pick disease type C1 (NPC1)	132:166	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	42	theme	rare	173:176	arg1	disorder					215:222	a rare, prematurely fatal lysosomal storage disorder	171:222	a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood	171:361	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	7	43	theme	large	1261:1265	arg1	scale					1267:1271	such a large scale	1254:1271	such a large scale	1254:1271	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	3	44	theme	genome-wide	549:559	arg1	data					575:578	genome-wide transcriptome data	549:578	genome-wide transcriptome data	549:578	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	4	45	theme	extensive	624:632	arg1	cohort					634:639	an extensive cohort	621:639	an extensive cohort of 41 NPC1 patients	621:659	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	7	46	theme	potential	1413:1421	arg1	biomarkers					1423:1432	potential biomarkers	1413:1432	potential biomarkers	1413:1432	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	0	47	theme	onset	22:26	arg1	age					15:17	age	15:17	age of onset and clinical severity in Niemann-Pick disease type C1	15:80	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	7	48	theme	clinical	1293:1300	arg1	phenotypes					1315:1324	clinical and cellular phenotypes	1293:1324	clinical and cellular phenotypes	1293:1324	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	3	49	theme	transcriptome	561:573	arg1	data					575:578	genome-wide transcriptome data	549:578	genome-wide transcriptome data	549:578	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	4	50	theme	primary	596:602	arg1	fibroblasts					604:614	primary fibroblasts	596:614	primary fibroblasts from an extensive cohort of 41 NPC1 patients	596:659	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	0	51	theme	severity	41:48	arg1	age					15:17	age	15:17	age of onset and clinical severity in Niemann-Pick disease type C1	15:80	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	7	52	with	correlation	1276:1286	arg1	phenotypes					1315:1324	clinical and cellular phenotypes	1293:1324	clinical and cellular phenotypes	1293:1324	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	5	53	theme	onset	1035:1039	arg1	age					1028:1030	age	1028:1030	age of onset	1028:1039	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	5	53	theme	onset	1035:1039	arg1	defects					1017:1023	lysosomal defects	1007:1023	lysosomal defects	1007:1023	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	5	54	theme	particular	1045:1054	arg1	onset					1077:1081	particular neurological symptom onset	1045:1081	particular neurological symptom onset	1045:1081	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	6	55	theme	Hierarchical	1084:1095	arg1	clustering					1097:1106	Hierarchical clustering	1084:1106	Hierarchical clustering	1084:1106	Hierarchical clustering showed that these genes exhibited distinct expression patterns among patient subgroups.
35140266	0	56	from	Correlation	0:10	arg1	C1					79:80	Niemann-Pick disease type C1	53:80	Niemann-Pick disease type C1	53:80	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	6	57	theme	distinct	1142:1149	arg1	patterns					1162:1169	distinct expression patterns	1142:1169	distinct expression patterns among patient subgroups	1142:1193	Hierarchical clustering showed that these genes exhibited distinct expression patterns among patient subgroups.
35140266	5	58	theme	symptom	1069:1075	arg1	onset					1077:1081	particular neurological symptom onset	1045:1081	particular neurological symptom onset	1045:1081	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	7	59	theme	rich	1339:1342	arg1	resource					1352:1359	a rich genomic resource	1337:1359	a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets	1337:1475	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	0	60	with	Correlation	0:10	arg1	expression					120:129	gene expression	115:129	gene expression	115:129	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	0	60	with	Correlation	0:10	arg1	abnormalities					97:109	lysosomal abnormalities	87:109	lysosomal abnormalities	87:109	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	5	61	theme	clinical	868:875	arg1	parameters					877:886	these clinical parameters	862:886	these clinical parameters	862:886	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	4	62	theme	quantitative	724:735	arg1	LysoTracker					743:753	the lysosomal quantitative probe LysoTracker	710:753	the lysosomal quantitative probe LysoTracker	710:753	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	4	62	theme	quantitative	724:735	arg1	predictor					772:780	a predictor	770:780	a predictor for age of onset and disease severity	770:818	In this study, primary fibroblasts from an extensive cohort of 41 NPC1 patients were used to validate our previous findings that the lysosomal quantitative probe LysoTracker can be used as a predictor for age of onset and disease severity.
35140266	5	63	theme	lysosomal	1007:1015	arg1	defects					1017:1023	lysosomal defects	1007:1023	lysosomal defects	1007:1023	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	6	64	theme	expression	1151:1160	arg1	patterns					1162:1169	distinct expression patterns	1142:1169	distinct expression patterns among patient subgroups	1142:1193	Hierarchical clustering showed that these genes exhibited distinct expression patterns among patient subgroups.
35140266	7	65	theme	genomic	1344:1350	arg1	resource					1352:1359	a rich genomic resource	1337:1359	a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets	1337:1475	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	1	66	theme	lysosomal	197:205	arg1	disease					145:151	Niemann-Pick disease	132:151	Niemann-Pick disease type C1 (NPC1)	132:166	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	66	theme	lysosomal	197:205	arg1	disorder					215:222	a rare, prematurely fatal lysosomal storage disorder	171:222	a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood	171:361	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	0	67	theme	Niemann-Pick	53:64	arg1	C1					79:80	Niemann-Pick disease type C1	53:80	Niemann-Pick disease type C1	53:80	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	0	68	from	C1	79:80	arg1	Correlation					0:10	Correlation	0:10	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.	0:130	Correlation of age of onset and clinical severity in Niemann-Pick disease type C1 with lysosomal abnormalities and gene expression.
35140266	7	69	theme	cellular	1306:1313	arg1	phenotypes					1315:1324	clinical and cellular phenotypes	1293:1324	clinical and cellular phenotypes	1293:1324	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	1	70	theme	Niemann-Pick	132:143	arg1	disease					145:151	Niemann-Pick disease	132:151	Niemann-Pick disease type C1 (NPC1)	132:166	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	70	theme	Niemann-Pick	132:143	arg1	disorder					215:222	a rare, prematurely fatal lysosomal storage disorder	171:222	a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood	171:361	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	70	theme	Niemann-Pick	132:143	arg1	NPC1					162:165	NPC1	162:165	NPC1	162:165	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	3	71	from	factor	529:534	arg1	addition					466:473	addition	466:473	addition	466:473	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	1	72	theme	storage	207:213	arg1	disease					145:151	Niemann-Pick disease	132:151	Niemann-Pick disease type C1 (NPC1)	132:166	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	72	theme	storage	207:213	arg1	disorder					215:222	a rare, prematurely fatal lysosomal storage disorder	171:222	a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood	171:361	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	73	theme	wide-ranging	301:312	arg1	age					314:316	a wide-ranging age	299:316	highly variable severity and disease progression as well as a wide-ranging age of onset	239:325	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	1	74	dep	rare	173:176	arg1	fatal					191:195	fatal	191:195	fatal	191:195	Niemann-Pick disease type C1 (NPC1) is a rare, prematurely fatal lysosomal storage disorder which exhibits highly variable severity and disease progression as well as a wide-ranging age of onset, from perinatal stages to adulthood.
35140266	7	75	theme	transcriptomic	1231:1244	arg1	data					1246:1249	transcriptomic data	1231:1249	transcriptomic data on such a large scale	1231:1271	This study is the first to collect transcriptomic data on such a large scale in correlation with clinical and cellular phenotypes, providing a rich genomic resource to address NPC1 clinical heterogeneity and discover potential biomarkers, disease modifiers, or therapeutic targets.
35140266	3	76	theme	NPC1	482:485	arg1	sizes					502:506	small NPC1 patient sample sizes	476:506	small NPC1 patient sample sizes	476:506	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	3	76	theme	NPC1	482:485	arg1	factor					529:534	a limiting factor	518:534	a limiting factor	518:534	In addition, small NPC1 patient sample sizes have been a limiting factor in acquiring genome-wide transcriptome data.
35140266	5	77	from	fibroblasts	925:935	arg1	data					907:910	RNA expression data	892:910	RNA expression data	892:910	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35140266	5	77	from	fibroblasts	925:935	arg1	parameters					877:886	these clinical parameters	862:886	these clinical parameters	862:886	We also examined the correlation between these clinical parameters and RNA expression data from primary fibroblasts and identified a set of genes that were significantly associated with lysosomal defects or age of onset, in particular neurological symptom onset.
35684266	5	0	theme	G+	601:602	arg1	bacteria					689:696	three G- bacteria	680:696	three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica)	680:767	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	0	theme	G+	601:602	arg1	bacteria					604:611	Three G+ bacteria	595:611	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis)	595:677	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	0	theme	G+	601:602	arg1	organisms					863:871	model organisms	857:871	model organisms	857:871	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	0	theme	G+	601:602	arg1	yeasts					779:784	four yeasts	774:784	four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans)	774:842	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	8	1	theme	selected	1089:1096	arg1	elements					1098:1105	selected elements	1089:1105	selected elements	1089:1105	The content of selected elements was determined in seaweed samples by ICP-OES.
35684266	7	2	theme	seaweeds	986:993	arg1	concentrations					955:968	The minimum inhibitory concentrations	932:968	The minimum inhibitory concentrations of the selected seaweeds	932:993	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	1	3	theme	antioxidant	154:164	arg1	activity					184:191	the antioxidant and antimicrobial activity	150:191	activity	184:191	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	1	4	theme	antimicrobial	170:182	arg1	activity					184:191	the antioxidant and antimicrobial activity	150:191	activity	184:191	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	12	5	theme	present	1459:1465	arg1	substances					1448:1457	the active substances	1437:1457	the active substances present in the algae that are responsible for antioxidant and antimicrobial activity	1437:1542	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	5	6	dep	bacteria	689:696	arg1	enterica					710:717	Salmonella enterica	699:717	Salmonella enterica	699:717	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	6	dep	bacteria	689:696	arg1	aeruginosa					732:741	Pseudomonas aeruginosa	720:741	Pseudomonas aeruginosa	720:741	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	6	dep	bacteria	689:696	arg1	enterocolitica					753:766	Yersinia enterocolitica	744:766	Yersinia enterocolitica	744:766	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	4	7	theme	MIC	583:585	arg1	method					587:592	MIC method	583:592	MIC method	583:592	Antimicrobial activity was analyzed by disk diffusion method and MIC method.
35684266	13	8	theme	antimicrobial	1588:1600	arg1	activity					1618:1625	the antimicrobial and antioxidant activity	1584:1625	the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content	1584:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	7	9	theme	minimum	936:942	arg1	concentrations					955:968	The minimum inhibitory concentrations	932:968	The minimum inhibitory concentrations of the selected seaweeds	932:993	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	1	10	from	determination	194:206	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	13	11	theme	antioxidant	1606:1616	arg1	activity					1618:1625	the antimicrobial and antioxidant activity	1584:1625	the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content	1584:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	12	12	from	algae	1474:1478	arg1	present					1459:1465	present	1459:1465	present	1459:1465	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	1	13	from	activity	184:191	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	12	14	theme	active	1441:1446	arg1	substances					1448:1457	the active substances	1437:1457	the active substances present in the algae that are responsible for antioxidant and antimicrobial activity	1437:1542	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	12	15	theme	seaweeds	1406:1413	arg1	investigation					1389:1401	Further investigation	1381:1401	Further investigation of seaweeds	1381:1413	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	5	16	theme	G-	686:687	arg1	organisms					863:871	model organisms	857:871	model organisms	857:871	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	16	theme	G-	686:687	arg1	bacteria					604:611	Three G+ bacteria	595:611	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis)	595:677	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	16	theme	G-	686:687	arg1	bacteria					689:696	three G- bacteria	680:696	three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica)	680:767	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	16	theme	G-	686:687	arg1	yeasts					779:784	four yeasts	774:784	four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans)	774:842	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	0	17	theme	Antioxidant	17:27	arg1	Elements					71:78	Elements	71:78	Elements	71:78	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	0	17	theme	Antioxidant	17:27	arg1	Activity					44:51	Antimicrobial Activity	30:51	Antimicrobial Activity	30:51	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	0	17	theme	Antioxidant	17:27	arg1	Determination					0:12	Determination	0:12	Determination of Antioxidant	0:27	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	0	17	theme	Antioxidant	17:27	arg1	Metals					60:65	Heavy Metals	54:65	Heavy Metals	54:65	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	6	18	theme	zones	897:901	arg1	size					878:881	The size	874:881	The size of inhibition zones	874:901	The size of inhibition zones ranged from 0.00 to 8.67 mm.
35684266	13	19	theme	due	1762:1764	arg1	risk					1757:1760	the health risk	1746:1760	the health risk due to heavy metals content	1746:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	0	20	theme	Elements	71:78	arg1	Content					80:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content	0:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.	0:107	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	2	21	dep	2641.34	409:415	arg1	to					406:407	to	406:407	to	406:407	Antioxidant activity was determined by DPPH method and the activity ranged from 0.00 to 2641.34 TEAC.
35684266	6	22	dep	8.67	923:926	arg1	to					920:921	to	920:921	to	920:921	The size of inhibition zones ranged from 0.00 to 8.67 mm.
35684266	13	23	theme	heavy	1769:1773	arg1	content					1782:1788	heavy metals content	1769:1788	heavy metals content	1769:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	9	24	theme	chemical	1157:1164	arg1	composition					1166:1176	The chemical composition	1153:1176	The chemical composition of the algae	1153:1189	The chemical composition of the algae showed differences between species and the presence of heavy metals.
35684266	5	25	theme	model	857:861	arg1	bacteria					689:696	three G- bacteria	680:696	three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica)	680:767	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	25	theme	model	857:861	arg1	bacteria					604:611	Three G+ bacteria	595:611	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis)	595:677	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	25	theme	model	857:861	arg1	organisms					863:871	model organisms	857:871	model organisms	857:871	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	25	theme	model	857:861	arg1	yeasts					779:784	four yeasts	774:784	four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans)	774:842	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	26	dep	bacteria	604:611	arg1	aureus					629:634	Staphylococcus aureus	614:634	Staphylococcus aureus	614:634	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	26	dep	bacteria	604:611	arg1	faecalis					650:657	Enterococcus faecalis	637:657	Enterococcus faecalis	637:657	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	26	dep	bacteria	604:611	arg1	subtilis					669:676	Bacillus subtilis	660:676	Bacillus subtilis	660:676	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	27	used	used	849:852	arg2	bacteria					604:611	Three G+ bacteria	595:611	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis)	595:677	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	27	used	used	849:852	arg2	bacteria					689:696	three G- bacteria	680:696	three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica)	680:767	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	27	used	used	849:852	arg2	organisms					863:871	model organisms	857:871	model organisms	857:871	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	27	used	used	849:852	arg2	yeasts					779:784	four yeasts	774:784	four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans)	774:842	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	0	28	theme	Extracts	99:106	arg1	Content					80:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content	0:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.	0:107	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	13	29	from	activity	1618:1625	arg1	seaweeds					1667:1674	five commonly consumed seaweeds	1644:1674	five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content	1644:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	13	30	theme	health	1750:1755	arg1	risk					1757:1760	the health risk	1746:1760	the health risk due to heavy metals content	1746:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	0	31	theme	Determination	0:12	arg1	Content					80:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content	0:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.	0:107	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	9	32	theme	algae	1185:1189	arg1	composition					1166:1176	The chemical composition	1153:1176	The chemical composition of the algae	1153:1189	The chemical composition of the algae showed differences between species and the presence of heavy metals.
35684266	14	33	theme	antioxidant	1866:1876	arg1	activity					1878:1885	antimicrobial and antioxidant activity	1848:1885	antimicrobial and antioxidant activity	1848:1885	Our study contributes to the evidence that seaweeds have antimicrobial and antioxidant activity and seaweed extracts have for pharmacological applications.
35684266	13	34	theme	selected	1705:1712	arg1	microorganisms					1714:1727	selected microorganisms	1705:1727	selected microorganisms	1705:1727	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	7	35	theme	MIC50	1037:1041	arg1	µL/mL					1066:1070	MIC90 5.26 µL/mL	1055:1070	MIC90 5.26 µL/mL	1055:1070	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	7	35	theme	MIC50	1037:1041	arg1	µL/mL					1048:1052	MIC50 3.43 µL/mL	1037:1052	MIC50 3.43 µL/mL (MIC90 5.26 µL/mL)	1037:1071	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	14	36	theme	antimicrobial	1848:1860	arg1	activity					1878:1885	antimicrobial and antioxidant activity	1848:1885	antimicrobial and antioxidant activity	1848:1885	Our study contributes to the evidence that seaweeds have antimicrobial and antioxidant activity and seaweed extracts have for pharmacological applications.
35684266	0	37	theme	Metals	60:65	arg1	Content					80:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content	0:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.	0:107	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	0	38	theme	Antimicrobial	30:42	arg1	Activity					44:51	Antimicrobial Activity	30:51	Antimicrobial Activity	30:51	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	12	39	from	present	1459:1465	arg1	responsible					1489:1499	responsible	1489:1499	responsible	1489:1499	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	12	39	from	present	1459:1465	arg1	algae					1474:1478	the algae	1470:1478	the algae that are responsible for antioxidant and antimicrobial activity	1470:1542	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	14	40	contain	have	1843:1846	arg2	activity					1878:1885	antimicrobial and antioxidant activity	1848:1885	antimicrobial and antioxidant activity	1848:1885	Our study contributes to the evidence that seaweeds have antimicrobial and antioxidant activity and seaweed extracts have for pharmacological applications.
35684266	14	40	contain	have	1843:1846	arg1	seaweeds					1834:1841	seaweeds	1834:1841	seaweeds	1834:1841	Our study contributes to the evidence that seaweeds have antimicrobial and antioxidant activity and seaweed extracts have for pharmacological applications.
35684266	5	41	dep	yeasts	779:784	arg1	krusei					810:815	C. krusei	807:815	C. krusei	807:815	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	41	dep	yeasts	779:784	arg1	tropicalis					795:804	Candida tropicalis	787:804	Candida tropicalis	787:804	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	14	42	theme	pharmacological	1917:1931	arg1	applications					1933:1944	pharmacological applications	1917:1944	pharmacological applications	1917:1944	Our study contributes to the evidence that seaweeds have antimicrobial and antioxidant activity and seaweed extracts have for pharmacological applications.
35684266	3	43	theme	highest	427:433	arg1	activity					447:454	The highest antioxidant activity	423:454	The highest antioxidant activity	423:454	The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC).
35684266	1	44	theme	chemical	211:218	arg1	elements					220:227	chemical elements	211:227	chemical elements	211:227	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	9	45	theme	metals	1252:1257	arg1	species					1218:1224	species	1218:1224	species	1218:1224	The chemical composition of the algae showed differences between species and the presence of heavy metals.
35684266	9	45	theme	metals	1252:1257	arg1	presence					1234:1241	the presence	1230:1241	the presence of heavy metals	1230:1257	The chemical composition of the algae showed differences between species and the presence of heavy metals.
35684266	1	46	theme	seaweed	249:255	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	4	47	theme	Antimicrobial	518:530	arg1	activity					532:539	Antimicrobial activity	518:539	Antimicrobial activity	518:539	Antimicrobial activity was analyzed by disk diffusion method and MIC method.
35684266	13	48	theme	consumed	1658:1665	arg1	seaweeds					1667:1674	five commonly consumed seaweeds	1644:1674	five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content	1644:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	7	49	theme	selected	977:984	arg1	seaweeds					986:993	the selected seaweeds	973:993	the selected seaweeds	973:993	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	12	50	theme	Further	1381:1387	arg1	investigation					1389:1401	Further investigation	1381:1401	Further investigation of seaweeds	1381:1413	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	5	51	theme	Candida	787:793	arg1	krusei					810:815	C. krusei	807:815	C. krusei	807:815	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	5	51	theme	Candida	787:793	arg1	tropicalis					795:804	Candida tropicalis	787:804	Candida tropicalis	787:804	Three G+ bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis), three G- bacteria (Salmonella enterica, Pseudomonas aeruginosa, Yersinia enterocolitica), and four yeasts (Candida tropicalis, C. krusei, C. glabrata, C. albicans) were used as model organisms.
35684266	8	52	theme	elements	1098:1105	arg1	content					1078:1084	The content	1074:1084	The content of selected elements	1074:1105	The content of selected elements was determined in seaweed samples by ICP-OES.
35684266	13	53	theme	extracts	1630:1637	arg1	activity					1618:1625	the antimicrobial and antioxidant activity	1584:1625	the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content	1584:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	7	54	theme	inhibitory	944:953	arg1	concentrations					955:968	The minimum inhibitory concentrations	932:968	The minimum inhibitory concentrations of the selected seaweeds	932:993	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	4	55	theme	diffusion	562:570	arg1	method					572:577	disk diffusion method	557:577	disk diffusion method	557:577	Antimicrobial activity was analyzed by disk diffusion method and MIC method.
35684266	1	56	from	metals	239:244	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	13	57	from	seaweeds	1667:1674	arg1	extracts					1630:1637	extracts	1630:1637	extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content	1630:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	13	57	from	seaweeds	1667:1674	arg1	activity					1618:1625	the antimicrobial and antioxidant activity	1584:1625	the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content	1584:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	8	58	theme	seaweed	1125:1131	arg1	samples					1133:1139	seaweed samples	1125:1139	seaweed samples	1125:1139	The content of selected elements was determined in seaweed samples by ICP-OES.
35684266	13	59	theme	metals	1775:1780	arg1	content					1782:1788	heavy metals content	1769:1788	heavy metals content	1769:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	3	60	located	observed	460:467	arg1	TEAC					511:514	2457.5 TEAC	504:514	2457.5 TEAC	504:514	The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC).
35684266	3	60	located	observed	460:467	arg1	kombu					472:476	kombu	472:476	kombu (2641.34 TEAC)	472:491	The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC).
35684266	3	60	located	observed	460:467	arg1	TEAC					487:490	2641.34 TEAC	479:490	2641.34 TEAC	479:490	The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC).
35684266	3	60	located	observed	460:467	arg1	arame					497:501	arame	497:501	arame (2457.5 TEAC)	497:515	The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC).
35684266	3	60	located	observed	460:467	arg2	activity					447:454	The highest antioxidant activity	423:454	The highest antioxidant activity	423:454	The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC).
35684266	1	61	theme	research	124:131	arg1	aim					113:115	The aim	109:115	The aim of the research	109:131	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	1	62	theme	kombu	302:306	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	1	63	theme	hijiki	313:318	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	6	64	theme	inhibition	886:895	arg1	zones					897:901	inhibition zones	886:901	inhibition zones	886:901	The size of inhibition zones ranged from 0.00 to 8.67 mm.
35684266	0	65	theme	Seaweed	91:97	arg1	Extracts					99:106	Seaweed Extracts	91:106	Seaweed Extracts	91:106	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	14	66	theme	seaweed	1891:1897	arg1	extracts					1899:1906	seaweed extracts	1891:1906	seaweed extracts	1891:1906	Our study contributes to the evidence that seaweeds have antimicrobial and antioxidant activity and seaweed extracts have for pharmacological applications.
35684266	12	67	theme	antioxidant	1505:1515	arg1	activity					1535:1542	antioxidant and antimicrobial activity	1505:1542	activity	1535:1542	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	1	68	theme	elements	220:227	arg1	determination					194:206	determination	194:206	determination of chemical elements	194:227	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	1	68	theme	elements	220:227	arg1	metals					239:244	heavy metals	233:244	heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	233:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	1	68	theme	elements	220:227	arg1	activity					184:191	the antioxidant and antimicrobial activity	150:191	activity	184:191	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	7	69	theme	3.43	1043:1046	arg1	µL/mL					1066:1070	MIC90 5.26 µL/mL	1055:1070	MIC90 5.26 µL/mL	1055:1070	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	7	69	theme	3.43	1043:1046	arg1	µL/mL					1048:1052	MIC50 3.43 µL/mL	1037:1052	MIC50 3.43 µL/mL (MIC90 5.26 µL/mL)	1037:1071	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	12	70	theme	antimicrobial	1521:1533	arg1	activity					1535:1542	antioxidant and antimicrobial activity	1505:1542	activity	1535:1542	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	11	71	dep	76.48	1368:1372	arg1	to					1365:1366	to	1365:1366	to	1365:1366	All seaweed samples contained arsenic ranging from 6.6546 to 76.48 mg/kg.
35684266	11	72	theme	seaweed	1311:1317	arg1	samples					1319:1325	All seaweed samples	1307:1325	All seaweed samples	1307:1325	All seaweed samples contained arsenic ranging from 6.6546 to 76.48 mg/kg.
35684266	1	73	theme	wakame	269:274	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	13	74	dep	ability	1686:1692	arg1	determine					1736:1744	determine	1736:1744	to determine the health risk due to heavy metals content	1733:1788	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	13	74	dep	ability	1686:1692	arg1	inhibit					1697:1703	inhibit	1697:1703	to inhibit selected microorganisms	1694:1727	This study was carried out to evaluate the antimicrobial and antioxidant activity of extracts from five commonly consumed seaweeds for their ability to inhibit selected microorganisms and to determine the health risk due to heavy metals content.
35684266	0	75	theme	Activity	44:51	arg1	Content					80:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content	0:86	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.	0:107	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	1	76	theme	arame	277:281	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	2	77	theme	DPPH	360:363	arg1	method					365:370	DPPH method	360:370	DPPH method	360:370	Antioxidant activity was determined by DPPH method and the activity ranged from 0.00 to 2641.34 TEAC.
35684266	12	78	attach	present	1459:1465	arg1	responsible					1489:1499	responsible	1489:1499	responsible	1489:1499	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	12	78	attach	present	1459:1465	arg2	substances					1448:1457	the active substances	1437:1457	the active substances present in the algae that are responsible for antioxidant and antimicrobial activity	1437:1542	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	12	78	attach	present	1459:1465	arg1	algae					1474:1478	the algae	1470:1478	the algae that are responsible for antioxidant and antimicrobial activity	1470:1542	Further investigation of seaweeds is needed to identify the active substances present in the algae that are responsible for antioxidant and antimicrobial activity.
35684266	7	79	theme	MIC90	1055:1059	arg1	µL/mL					1066:1070	MIC90 5.26 µL/mL	1055:1070	MIC90 5.26 µL/mL	1055:1070	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	7	79	theme	MIC90	1055:1059	arg1	µL/mL					1048:1052	MIC50 3.43 µL/mL	1037:1052	MIC50 3.43 µL/mL (MIC90 5.26 µL/mL)	1037:1071	The minimum inhibitory concentrations of the selected seaweeds ranged from MIC50 98.46 (MIC90 100.25) to MIC50 3.43 µL/mL (MIC90 5.26 µL/mL).
35684266	0	80	theme	Heavy	54:58	arg1	Metals					60:65	Heavy Metals	54:65	Heavy Metals	54:65	Determination of Antioxidant, Antimicrobial Activity, Heavy Metals and Elements Content of Seaweed Extracts.
35684266	1	81	theme	dulse	284:288	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	1	82	theme	laminaria	291:299	arg1	extracts					257:264	seaweed extracts	249:264	seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	249:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
35684266	11	83	contain	contained	1327:1335	arg2	arsenic					1337:1343	arsenic	1337:1343	arsenic ranging from 6.6546 to 76.48 mg/kg	1337:1378	All seaweed samples contained arsenic ranging from 6.6546 to 76.48 mg/kg.
35684266	11	83	contain	contained	1327:1335	arg1	samples					1319:1325	All seaweed samples	1307:1325	All seaweed samples	1307:1325	All seaweed samples contained arsenic ranging from 6.6546 to 76.48 mg/kg.
35684266	3	84	theme	antioxidant	435:445	arg1	activity					447:454	The highest antioxidant activity	423:454	The highest antioxidant activity	423:454	The highest antioxidant activity was observed in kombu (2641.34 TEAC) and arame (2457.5 TEAC).
35684266	9	85	theme	heavy	1246:1250	arg1	metals					1252:1257	heavy metals	1246:1257	heavy metals	1246:1257	The chemical composition of the algae showed differences between species and the presence of heavy metals.
35684266	4	86	theme	disk	557:560	arg1	method					572:577	disk diffusion method	557:577	disk diffusion method	557:577	Antimicrobial activity was analyzed by disk diffusion method and MIC method.
35684266	2	87	theme	Antioxidant	321:331	arg1	activity					333:340	Antioxidant activity	321:340	Antioxidant activity	321:340	Antioxidant activity was determined by DPPH method and the activity ranged from 0.00 to 2641.34 TEAC.
35684266	1	88	theme	heavy	233:237	arg1	metals					239:244	heavy metals	233:244	heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki	233:318	The aim of the research was to determine the antioxidant and antimicrobial activity, determination of chemical elements and heavy metals in seaweed extracts of wakame, arame, dulse, laminaria, kombu, and hijiki.
37317145	13	0	dep	=	1481:1481	arg1	CI					1467:1468	CI): 1.349	1467:1476	CI): 1.349	1467:1476	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	2	1	theme	Preterm	96:102	arg1	birth					104:108	Preterm birth	96:108	Preterm birth	96:108	Preterm birth is a major challenge in pregnancy worldwide.
37317145	2	1	theme	Preterm	96:102	arg1	challenge					121:129	a major challenge	113:129	a major challenge in pregnancy worldwide	113:152	Preterm birth is a major challenge in pregnancy worldwide.
37317145	16	2	theme	glycan	1920:1925	arg1	metabolism					1927:1936	dysregulated glycan metabolism	1907:1936	dysregulated glycan metabolism	1907:1936	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	7	3	theme	RNA	695:697	arg1	gene					699:702	the 16S ribosomal RNA gene	677:702	the 16S ribosomal RNA gene	677:702	Fecal samples were freshly collected at 24-28 weeks of gestation before delivery, and the 16S ribosomal RNA gene was sequenced.
37317145	15	4	theme	Communities	1734:1744	arg1	Investigation					1717:1729	the Investigation	1713:1729	the Investigation of Communities	1713:1744	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	5	5	theme	spontaneous	452:462	arg1	sPTB					479:482	sPTB	479:482	sPTB	479:482	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	5	5	theme	spontaneous	452:462	arg1	birth					472:476	spontaneous preterm birth	452:476	spontaneous preterm birth (sPTB)	452:483	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	7	6	theme	16S	681:683	arg1	gene					699:702	the 16S ribosomal RNA gene	677:702	the 16S ribosomal RNA gene	677:702	Fecal samples were freshly collected at 24-28 weeks of gestation before delivery, and the 16S ribosomal RNA gene was sequenced.
37317145	2	7	theme	major	115:119	arg1	birth					104:108	Preterm birth	96:108	Preterm birth	96:108	Preterm birth is a major challenge in pregnancy worldwide.
37317145	2	7	theme	major	115:119	arg1	challenge					121:129	a major challenge	113:129	a major challenge in pregnancy worldwide	113:152	Preterm birth is a major challenge in pregnancy worldwide.
37317145	6	8	theme	mother-child	564:575	arg1	study					584:588	this mother-child cohort study	559:588	this mother-child cohort study	559:588	Two hundred eleven women carrying singleton pregnancies were enrolled in this mother-child cohort study.
37317145	10	9	theme	BMI	1022:1024	arg1	over-weight					1009:1019	over-weight	1009:1019	over-weight (BMI ≥ 24) before pregnancy	1009:1047	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	10	9	theme	BMI	1022:1024	arg1	≥					1026:1026	BMI ≥ 24	1022:1029	BMI ≥ 24	1022:1029	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	3	10	theme	severe	226:231	arg1	complications					233:245	severe complications	226:245	severe complications	226:245	Prematurity is the leading cause of death in infants and may result in severe complications.
37317145	16	11	theme	Maternal	1805:1812	arg1	microbiota					1818:1827	Maternal gut microbiota	1805:1827	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism	1805:1936	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	9	12	from	questionnaires	935:948	arg1	records					891:897	records	891:897	records from the Medical Birth Registry and questionnaires	891:948	Demographic characteristics were collected using records from the Medical Birth Registry and questionnaires.
37317145	6	13	theme	singleton	520:528	arg1	pregnancies					530:540	singleton pregnancies	520:540	singleton pregnancies	520:540	Two hundred eleven women carrying singleton pregnancies were enrolled in this mother-child cohort study.
37317145	8	14	theme	associated	777:786	arg1	pathways					799:806	associated functional pathways	777:806	associated functional pathways	777:806	Microbial diversity and composition, core microbiome, and associated functional pathways were then statistically analyzed.
37317145	10	15	dep	lower	1054:1058	arg1	diversity					1066:1074	alpha diversity	1060:1074	alpha diversity	1060:1074	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	9	16	from	Registry	922:929	arg1	records					891:897	records	891:897	records from the Medical Birth Registry and questionnaires	891:948	Demographic characteristics were collected using records from the Medical Birth Registry and questionnaires.
37317145	12	17	theme	random	1268:1273	arg1	model					1282:1286	random forest model	1268:1286	random forest model	1268:1286	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	15	18	theme	glycan	1651:1656	arg1	biosynthesis					1658:1669	glycan biosynthesis	1651:1669	glycan biosynthesis	1651:1669	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	3	19	from	cause	182:186	arg1	infants					200:206	infants	200:206	infants	200:206	Prematurity is the leading cause of death in infants and may result in severe complications.
37317145	10	20	theme	alpha	1060:1064	arg1	diversity					1066:1074	alpha diversity	1060:1074	alpha diversity	1060:1074	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	5	21	theme	maternal	368:375	arg1	microbiome					381:390	the maternal gut microbiome	364:390	the maternal gut microbiome	364:390	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	8	22	theme	core	756:759	arg1	microbiome					761:770	core microbiome	756:770	core microbiome	756:770	Microbial diversity and composition, core microbiome, and associated functional pathways were then statistically analyzed.
37317145	9	23	theme	Demographic	842:852	arg1	characteristics					854:868	Demographic characteristics	842:868	Demographic characteristics	842:868	Demographic characteristics were collected using records from the Medical Birth Registry and questionnaires.
37317145	4	24	contain	have	306:309	arg2	causes					324:329	recognizable causes	311:329	recognizable causes	311:329	Nearly half of preterm births are spontaneous, but do not have recognizable causes.
37317145	4	24	contain	have	306:309	arg1	half					255:258	Nearly half	248:258	Nearly half of preterm births	248:276	Nearly half of preterm births are spontaneous, but do not have recognizable causes.
37317145	7	25	theme	Fecal	591:595	arg1	samples					597:603	Fecal samples	591:603	Fecal samples	591:603	Fecal samples were freshly collected at 24-28 weeks of gestation before delivery, and the 16S ribosomal RNA gene was sequenced.
37317145	6	26	contain	carrying	511:518	arg1	women					505:509	Two hundred eleven women	486:509	Two hundred eleven women carrying singleton pregnancies	486:540	Two hundred eleven women carrying singleton pregnancies were enrolled in this mother-child cohort study.
37317145	6	26	contain	carrying	511:518	arg2	pregnancies					530:540	singleton pregnancies	520:540	singleton pregnancies	520:540	Two hundred eleven women carrying singleton pregnancies were enrolled in this mother-child cohort study.
37317145	13	27	theme	3.274	1435:1439	arg1	%					1444:1444	3.274 [95%	1435:1444	3.274 [95% confidence interval	1435:1464	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	13	27	theme	3.274	1435:1439	arg1	ratio					1403:1407	the odds ratio	1394:1407	the odds ratio of premature delivery	1394:1429	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	14	28	theme	Actinomyces	1603:1613	arg1	enrichment					1589:1598	The enrichment	1585:1598	The enrichment of Actinomyces	1585:1613	The enrichment of Actinomyces spp.
37317145	16	29	theme	Actinomyces	1885:1895	arg1	spp.					1897:1900	Actinomyces spp.	1885:1900	Actinomyces spp.	1885:1900	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	4	30	theme	births	271:276	arg1	half					255:258	Nearly half	248:258	Nearly half of preterm births	248:276	Nearly half of preterm births are spontaneous, but do not have recognizable causes.
37317145	13	31	theme	Actinomyces	1568:1578	arg1	spp					1580:1582	Actinomyces spp	1568:1582	Actinomyces spp	1568:1582	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	1	32	theme	Spontaneous	69:79	arg1	Birth					89:93	Spontaneous Preterm Birth	69:93	Spontaneous Preterm Birth	69:93	in the Gut Is Associated with Spontaneous Preterm Birth.
37317145	12	33	from	age	1335:1337	arg1	sPTB					1342:1345	sPTB	1342:1345	sPTB	1342:1345	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	13	34	theme	cutoff	1544:1549	arg1	>					1556:1556	a cutoff Hit% > 0.022	1542:1562	a cutoff Hit% > 0.022 for Actinomyces spp	1542:1582	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	16	35	theme	dysregulated	1907:1918	arg1	metabolism					1927:1936	dysregulated glycan metabolism	1907:1936	dysregulated glycan metabolism	1907:1936	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	13	36	theme	%	1554:1554	arg1	>					1556:1556	a cutoff Hit% > 0.022	1542:1562	a cutoff Hit% > 0.022 for Actinomyces spp	1542:1582	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	15	37	from	Investigation	1717:1729	arg1	prediction					1697:1706	prediction	1697:1706	prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform	1697:1802	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	13	38	theme	delivery	1422:1429	arg1	%					1444:1444	3.274 [95%	1435:1444	3.274 [95% confidence interval	1435:1464	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	13	38	theme	delivery	1422:1429	arg1	ratio					1403:1407	the odds ratio	1394:1407	the odds ratio of premature delivery	1394:1429	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	0	39	theme	Actinomyces	22:32	arg1	Abundance					9:17	A Higher Abundance	0:17	A Higher Abundance of Actinomyces	0:32	A Higher Abundance of Actinomyces spp.
37317145	13	40	with	over-weight	1508:1518	arg1	>					1556:1556	a cutoff Hit% > 0.022	1542:1562	a cutoff Hit% > 0.022 for Actinomyces spp	1542:1582	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	16	41	theme	sPTB	1961:1964	arg1	risk					1966:1969	sPTB risk	1961:1969	sPTB risk	1961:1969	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	2	42	theme	pregnancy	134:142	arg1	worldwide					144:152	pregnancy worldwide	134:152	pregnancy worldwide	134:152	Preterm birth is a major challenge in pregnancy worldwide.
37317145	13	43	with	group	1497:1501	arg1	over-weight					1508:1518	over-weight	1508:1518	over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp	1508:1582	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	4	44	theme	recognizable	311:322	arg1	causes					324:329	recognizable causes	311:329	recognizable causes	311:329	Nearly half of preterm births are spontaneous, but do not have recognizable causes.
37317145	13	45	theme	odds	1398:1401	arg1	%					1444:1444	3.274 [95%	1435:1444	3.274 [95% confidence interval	1435:1464	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	13	45	theme	odds	1398:1401	arg1	ratio					1403:1407	the odds ratio	1394:1407	the odds ratio of premature delivery	1394:1429	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	16	46	theme	alpha	1845:1849	arg1	diversity					1851:1859	a lower alpha diversity	1837:1859	a lower alpha diversity	1837:1859	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	13	47	theme	multivariate	1352:1363	arg1	model					1376:1380	The multivariate regression model	1348:1380	The multivariate regression model	1348:1380	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	10	48	theme	gut	978:980	arg1	microbiome					982:991	the gut microbiome	974:991	the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy	974:1047	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	9	49	theme	Birth	916:920	arg1	Registry					922:929	the Medical Birth Registry	904:929	the Medical Birth Registry	904:929	Demographic characteristics were collected using records from the Medical Birth Registry and questionnaires.
37317145	7	50	theme	ribosomal	685:693	arg1	gene					699:702	the 16S ribosomal RNA gene	677:702	the 16S ribosomal RNA gene	677:702	Fecal samples were freshly collected at 24-28 weeks of gestation before delivery, and the 16S ribosomal RNA gene was sequenced.
37317145	12	51	theme	discriminant	1193:1204	arg1	LDA					1216:1218	LDA	1216:1218	LDA	1216:1218	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	12	51	theme	discriminant	1193:1204	arg1	analysis					1206:1213	the Linear discriminant analysis	1182:1213	the Linear discriminant analysis	1182:1213	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	5	52	theme	associated	396:405	arg1	pathways					418:425	associated functional pathways	396:425	associated functional pathways	396:425	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	3	53	theme	death	191:195	arg1	Prematurity					155:165	Prematurity	155:165	Prematurity	155:165	Prematurity is the leading cause of death in infants and may result in severe complications.
37317145	3	53	theme	death	191:195	arg1	cause					182:186	the leading cause	170:186	the leading cause of death in infants	170:206	Prematurity is the leading cause of death in infants and may result in severe complications.
37317145	5	54	theme	preterm	464:470	arg1	sPTB					479:482	sPTB	479:482	sPTB	479:482	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	5	54	theme	preterm	464:470	arg1	birth					472:476	spontaneous preterm birth	452:476	spontaneous preterm birth (sPTB)	452:483	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	6	55	theme	cohort	577:582	arg1	study					584:588	this mother-child cohort study	559:588	this mother-child cohort study	559:588	Two hundred eleven women carrying singleton pregnancies were enrolled in this mother-child cohort study.
37317145	15	56	theme	platform	1795:1802	arg1	Reconstruction					1749:1762	Reconstruction	1749:1762	Reconstruction of Unobserved States (PICRUSt) platform	1749:1802	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	5	57	theme	key	440:442	arg1	role					444:447	a key role	438:447	a key role	438:447	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	12	58	theme	gestational	1323:1333	arg1	age					1335:1337	gestational age	1323:1337	gestational age in sPTB	1323:1345	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	15	59	from	biosynthesis	1658:1669	arg1	sPTB					1689:1692	sPTB	1689:1692	sPTB	1689:1692	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	16	60	theme	gut	1814:1816	arg1	microbiota					1818:1827	Maternal gut microbiota	1805:1827	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism	1805:1936	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	15	61	theme	States	1778:1783	arg1	platform					1795:1802	Unobserved States (PICRUSt) platform	1767:1802	Unobserved States (PICRUSt) platform	1767:1802	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	15	62	from	metabolism	1675:1684	arg1	sPTB					1689:1692	sPTB	1689:1692	sPTB	1689:1692	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	2	63	from	challenge	121:129	arg1	worldwide					144:152	pregnancy worldwide	134:152	pregnancy worldwide	134:152	Preterm birth is a major challenge in pregnancy worldwide.
37317145	7	64	theme	gestation	646:654	arg1	weeks					637:641	24-28 weeks	631:641	24-28 weeks of gestation	631:654	Fecal samples were freshly collected at 24-28 weeks of gestation before delivery, and the 16S ribosomal RNA gene was sequenced.
37317145	13	65	theme	interval	1457:1464	arg1	%					1444:1444	3.274 [95%	1435:1444	3.274 [95% confidence interval	1435:1464	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	13	65	theme	interval	1457:1464	arg1	ratio					1403:1407	the odds ratio	1394:1407	the odds ratio of premature delivery	1394:1429	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	13	66	from	%	1444:1444	arg1	group					1497:1501	the group	1493:1501	the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp	1493:1582	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	8	67	theme	Microbial	719:727	arg1	diversity					729:737	Microbial diversity	719:737	Microbial diversity	719:737	Microbial diversity and composition, core microbiome, and associated functional pathways were then statistically analyzed.
37317145	8	68	theme	functional	788:797	arg1	pathways					799:806	associated functional pathways	777:806	associated functional pathways	777:806	Microbial diversity and composition, core microbiome, and associated functional pathways were then statistically analyzed.
37317145	12	69	theme	forest	1275:1280	arg1	model					1282:1286	random forest model	1268:1286	random forest model	1268:1286	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	16	70	theme	spp.	1897:1900	arg1	diversity					1851:1859	a lower alpha diversity	1837:1859	a lower alpha diversity	1837:1859	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	16	70	theme	spp.	1897:1900	arg1	abundance					1872:1880	increased abundance	1862:1880	increased abundance of Actinomyces spp.	1862:1900	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	16	70	theme	spp.	1897:1900	arg1	metabolism					1927:1936	dysregulated glycan metabolism	1907:1936	dysregulated glycan metabolism	1907:1936	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	12	71	theme	Spearman	1242:1249	arg1	correlation					1251:1261	Spearman correlation	1242:1261	Spearman correlation	1242:1261	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	3	72	theme	leading	174:180	arg1	Prematurity					155:165	Prematurity	155:165	Prematurity	155:165	Prematurity is the leading cause of death in infants and may result in severe complications.
37317145	3	72	theme	leading	174:180	arg1	cause					182:186	the leading cause	170:186	the leading cause of death in infants	170:206	Prematurity is the leading cause of death in infants and may result in severe complications.
37317145	0	73	theme	Higher	2:7	arg1	Abundance					9:17	A Higher Abundance	0:17	A Higher Abundance of Actinomyces	0:32	A Higher Abundance of Actinomyces spp.
37317145	4	74	theme	preterm	263:269	arg1	births					271:276	preterm births	263:276	preterm births	263:276	Nearly half of preterm births are spontaneous, but do not have recognizable causes.
37317145	10	75	theme	mothers	996:1002	arg1	microbiome					982:991	the gut microbiome	974:991	the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy	974:1047	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	1	76	theme	Preterm	81:87	arg1	Birth					89:93	Spontaneous Preterm Birth	69:93	Spontaneous Preterm Birth	69:93	in the Gut Is Associated with Spontaneous Preterm Birth.
37317145	13	77	theme	Hit	1551:1553	arg1	>					1556:1556	a cutoff Hit% > 0.022	1542:1562	a cutoff Hit% > 0.022 for Actinomyces spp	1542:1582	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	13	78	theme	premature	1412:1420	arg1	delivery					1422:1429	premature delivery	1412:1429	premature delivery	1412:1429	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	10	79	theme	normal	1092:1097	arg1	BMI					1099:1101	normal BMI	1092:1101	normal BMI before pregnancy	1092:1118	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	9	80	theme	Medical	908:914	arg1	Registry					922:929	the Medical Birth Registry	904:929	the Medical Birth Registry	904:929	Demographic characteristics were collected using records from the Medical Birth Registry and questionnaires.
37317145	16	81	theme	lower	1839:1843	arg1	diversity					1851:1859	a lower alpha diversity	1837:1859	a lower alpha diversity	1837:1859	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	10	82	with	mothers	996:1002	arg1	over-weight					1009:1019	over-weight	1009:1019	over-weight (BMI ≥ 24) before pregnancy	1009:1047	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	10	82	with	mothers	996:1002	arg1	≥					1026:1026	BMI ≥ 24	1022:1029	BMI ≥ 24	1022:1029	The result showed that the gut microbiome of mothers with over-weight (BMI ≥ 24) before pregnancy have lower alpha diversity than those with normal BMI before pregnancy.
37317145	5	83	theme	functional	407:416	arg1	pathways					418:425	associated functional pathways	396:425	associated functional pathways	396:425	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
37317145	11	84	theme	higher	1123:1128	arg1	abundance					1130:1138	A higher abundance	1121:1138	A higher abundance of Actinomyces	1121:1153	A higher abundance of Actinomyces spp.
37317145	12	85	theme	effect	1221:1226	arg1	LEfSe					1234:1238	LEfSe	1234:1238	LEfSe	1234:1238	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	12	85	theme	effect	1221:1226	arg1	size					1228:1231	effect size	1221:1231	effect size (LEfSe)	1221:1239	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	11	86	theme	Actinomyces	1143:1153	arg1	abundance					1130:1138	A higher abundance	1121:1138	A higher abundance of Actinomyces	1121:1153	A higher abundance of Actinomyces spp.
37317145	16	87	theme	increased	1862:1870	arg1	abundance					1872:1880	increased abundance	1862:1880	increased abundance of Actinomyces spp.	1862:1900	Maternal gut microbiota showing a lower alpha diversity, increased abundance of Actinomyces spp., and dysregulated glycan metabolism may be associated with sPTB risk.
37317145	12	88	theme	Linear	1186:1191	arg1	LDA					1216:1218	LDA	1216:1218	LDA	1216:1218	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	12	88	theme	Linear	1186:1191	arg1	analysis					1206:1213	the Linear discriminant analysis	1182:1213	the Linear discriminant analysis	1182:1213	was filtered out from the Linear discriminant analysis (LDA) effect size (LEfSe), Spearman correlation, and random forest model, and was inversely correlated with gestational age in sPTB.
37317145	15	89	theme	Unobserved	1767:1776	arg1	PICRUSt					1786:1792	PICRUSt	1786:1792	PICRUSt	1786:1792	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	15	89	theme	Unobserved	1767:1776	arg1	States					1778:1783	Unobserved States	1767:1783	Unobserved States (PICRUSt) platform	1767:1802	was negatively correlated with glycan biosynthesis and metabolism in sPTB by prediction from the Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) platform.
37317145	13	90	theme	regression	1365:1374	arg1	model					1376:1380	The multivariate regression model	1348:1380	The multivariate regression model	1348:1380	The multivariate regression model showed that the odds ratio of premature delivery was 3.274 [95% confidence interval (CI): 1.349; p = 0.010] in the group with over-weight before pregnancy with a cutoff Hit% > 0.022 for Actinomyces spp.
37317145	5	91	theme	gut	377:379	arg1	microbiome					381:390	the maternal gut microbiome	364:390	the maternal gut microbiome	364:390	This study investigated whether the maternal gut microbiome and associated functional pathways might play a key role in spontaneous preterm birth (sPTB).
36740772	2	0	theme	volatile	400:407	arg1	acids					446:450	organic acids	438:450	organic acids	438:450	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	0	theme	volatile	400:407	arg1	substances					409:418	volatile substances	400:418	volatile substances	400:418	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	0	theme	volatile	400:407	arg1	polysaccharides					421:435	polysaccharides	421:435	polysaccharides	421:435	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	7	1	theme	GC × GC-TOF-MS	1515:1528	arg1	analyses					1530:1537	the GC-MS/O and GC × GC-TOF-MS analyses	1499:1537	the GC-MS/O and GC × GC-TOF-MS analyses	1499:1537	By the GC-MS/O and GC × GC-TOF-MS analyses, 196 volatile flavour compounds were identified, 51 odour-active areas discerned and 39 odourants determined.
36740772	8	2	theme	flavour	1686:1692	arg1	compounds					1694:1702	the flavour compounds	1682:1702	the flavour compounds that mainly contributed to the odour attributes of DG herb	1682:1761	It was terpenes and aromatics of the flavour compounds that mainly contributed to the odour attributes of DG herb.
36740772	6	3	theme	component	1316:1324	arg1	analysis					1326:1333	principal component analysis	1306:1333	a principal component analysis (PCA) model	1304:1345	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	6	3	theme	component	1316:1324	arg1	PCA					1336:1338	PCA	1336:1338	PCA	1336:1338	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	8	4	theme	DG	1755:1756	arg1	herb					1758:1761	DG herb	1755:1761	DG herb	1755:1761	It was terpenes and aromatics of the flavour compounds that mainly contributed to the odour attributes of DG herb.
36740772	7	5	theme	GC-MS/O	1503:1509	arg1	analyses					1530:1537	the GC-MS/O and GC × GC-TOF-MS analyses	1499:1537	the GC-MS/O and GC × GC-TOF-MS analyses	1499:1537	By the GC-MS/O and GC × GC-TOF-MS analyses, 196 volatile flavour compounds were identified, 51 odour-active areas discerned and 39 odourants determined.
36740772	0	6	theme	Volatile	0:7	arg1	identification					17:30	Volatile flavour identification	0:30	Volatile flavour identification	0:30	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	5	7	dep	METHODS	795:801	arg1	nose					814:817	Electronic nose	803:817	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	7	dep	METHODS	795:801	arg1	E-nose					820:825	E-nose	820:825	E-nose	820:825	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	8	theme	comprehensive	904:916	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	8	theme	comprehensive	904:916	arg1	spectrometry					973:984	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	spectrometry	973:984	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	6	9	theme	DG	1192:1193	arg1	samples					1195:1201	DG samples	1192:1201	DG samples cultivated in six provinces of China	1192:1238	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	0	10	theme	chromatography-mass	116:134	arg1	spectrometry/olfactometry					136:160	integrated gas chromatography-mass spectrometry/olfactometry	101:160	integrated gas chromatography-mass spectrometry/olfactometry	101:160	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	5	11	theme	chromatography-mass	844:862	arg1	GC × GC-TOF-MS					987:1000	GC × GC-TOF-MS	987:1000	GC × GC-TOF-MS	987:1000	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	11	theme	chromatography-mass	844:862	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	11	theme	chromatography-mass	844:862	arg1	GC-MS/O					891:897	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	GC-MS/O	891:897	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	10	12	theme	sinensis	2115:2122	arg1	compositions					2062:2073	flavour compositions	2054:2073	flavour compositions	2054:2073	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	10	12	theme	sinensis	2115:2122	arg1	complexity					2085:2094	odour complexity	2079:2094	odour complexity	2079:2094	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	9	13	theme	previous	1979:1986	arg1	report					1988:1993	any previous report	1975:1993	any previous report	1975:1993	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	10	14	theme	significant	2017:2027	arg1	step					2029:2032	a significant step	2015:2032	a significant step	2015:2032	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	10	15	dep	compositions	2062:2073	arg1	the					2050:2052	the	2050:2052	the	2050:2052	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	4	16	theme	DG	733:734	arg1	materials					736:744	DG materials	733:744	DG materials	733:744	OBJECTIVES This study was to determine volatile flavour compositions of DG materials and to reveal the odour complexity.
36740772	0	17	theme	two-dimensional	180:194	arg1	spectrometry					235:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	9	18	theme	SPME-GC × GC-TOF-MS	1779:1797	arg1	time					1820:1823	the first time	1810:1823	the first time employed to analyse the volatile flavours of DG materials	1810:1881	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	9	18	theme	SPME-GC × GC-TOF-MS	1779:1797	arg1	method					1799:1804	The SPME-GC × GC-TOF-MS method	1775:1804	The SPME-GC × GC-TOF-MS method	1775:1804	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	0	19	theme	chromatography-time-of-flight-mass	200:233	arg1	spectrometry					235:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	5	20	theme	materials	1112:1120	arg1	complexity					1095:1104	the flavour complexity	1083:1104	the flavour complexity of DG materials	1083:1120	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	3	21	theme	DG	640:641	arg1	quality					652:658	the DG material quality	636:658	the DG material quality	636:658	The fragrance is of importance to assessing the DG material quality.
36740772	11	22	theme	predetermined	2230:2242	arg1	compositions					2244:2255	predetermined compositions	2230:2255	predetermined compositions	2230:2255	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	4	23	theme	volatile	700:707	arg1	compositions					717:728	volatile flavour compositions	700:728	volatile flavour compositions of DG materials	700:744	OBJECTIVES This study was to determine volatile flavour compositions of DG materials and to reveal the odour complexity.
36740772	0	24	theme	Angelicae	62:70	arg1	sinensis					72:79	radix Angelicae sinensis	56:79	radix Angelicae sinensis	56:79	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	11	25	theme	statistical	2181:2191	arg1	model					2197:2201	a statistical PCA model	2179:2201	a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials	2179:2359	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	5	26	theme	gas	934:936	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	26	theme	gas	934:936	arg1	spectrometry					973:984	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	spectrometry	973:984	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	9	27	theme	first	1814:1818	arg1	time					1820:1823	the first time	1810:1823	the first time employed to analyse the volatile flavours of DG materials	1810:1881	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	9	27	theme	first	1814:1818	arg1	method					1799:1804	The SPME-GC × GC-TOF-MS method	1775:1804	The SPME-GC × GC-TOF-MS method	1775:1804	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	10	28	theme	flavour	2054:2060	arg1	compositions					2062:2073	flavour compositions	2054:2073	flavour compositions	2054:2073	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	0	29	theme	integrated	101:110	arg1	spectrometry/olfactometry					136:160	integrated gas chromatography-mass spectrometry/olfactometry	101:160	integrated gas chromatography-mass spectrometry/olfactometry	101:160	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	6	30	from	combination	1407:1417	arg1	sense					1489:1493	a statistical quantitative sense	1462:1493	a statistical quantitative sense	1462:1493	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	9	31	theme	volatile	1849:1856	arg1	flavours					1858:1865	the volatile flavours	1845:1865	the volatile flavours of DG materials	1845:1881	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	2	32	theme	organic	438:444	arg1	acids					446:450	organic acids	438:450	organic acids	438:450	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	32	theme	organic	438:444	arg1	vitamins					477:484	vitamins	477:484	vitamins	477:484	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	32	theme	organic	438:444	arg1	substances					409:418	volatile substances	400:418	volatile substances	400:418	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	32	theme	organic	438:444	arg1	microelements					487:499	microelements	487:499	microelements	487:499	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	32	theme	organic	438:444	arg1	ceramides					453:461	ceramides	453:461	ceramides	453:461	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	32	theme	organic	438:444	arg1	acids					470:474	amino acids	464:474	amino acids	464:474	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	6	33	theme	quantitative	1476:1487	arg1	sense					1489:1493	a statistical quantitative sense	1462:1493	a statistical quantitative sense	1462:1493	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	1	34	theme	typical	324:330	arg1	medicines					352:360	the typical traditional Chinese medicines	320:360	the typical traditional Chinese medicines	320:360	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	4	35	theme	odour	764:768	arg1	complexity					770:779	the odour complexity	760:779	the odour complexity	760:779	OBJECTIVES This study was to determine volatile flavour compositions of DG materials and to reveal the odour complexity.
36740772	5	36	theme	integrated	829:838	arg1	GC × GC-TOF-MS					987:1000	GC × GC-TOF-MS	987:1000	GC × GC-TOF-MS	987:1000	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	36	theme	integrated	829:838	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	36	theme	integrated	829:838	arg1	GC-MS/O					891:897	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	GC-MS/O	891:897	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	1	37	theme	Chinese	344:350	arg1	medicines					352:360	the typical traditional Chinese medicines	320:360	the typical traditional Chinese medicines	320:360	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	9	38	theme	materials	1873:1881	arg1	flavours					1858:1865	the volatile flavours	1845:1865	the volatile flavours of DG materials	1845:1881	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	5	39	theme	Electronic	803:812	arg1	nose					814:817	Electronic nose	803:817	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	39	theme	Electronic	803:812	arg1	E-nose					820:825	E-nose	820:825	E-nose	820:825	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	0	40	theme	odour	36:40	arg1	complexity					42:51	odour complexity	36:51	odour complexity	36:51	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	7	41	theme	flavour	1553:1559	arg1	compounds					1561:1569	196 volatile flavour compounds	1540:1569	196 volatile flavour compounds	1540:1569	By the GC-MS/O and GC × GC-TOF-MS analyses, 196 volatile flavour compounds were identified, 51 odour-active areas discerned and 39 odourants determined.
36740772	8	42	theme	compounds	1694:1702	arg1	terpenes					1656:1663	terpenes	1656:1663	terpenes	1656:1663	It was terpenes and aromatics of the flavour compounds that mainly contributed to the odour attributes of DG herb.
36740772	8	42	theme	compounds	1694:1702	arg1	aromatics					1669:1677	aromatics	1669:1677	aromatics	1669:1677	It was terpenes and aromatics of the flavour compounds that mainly contributed to the odour attributes of DG herb.
36740772	11	43	theme	quality	2326:2332	arg1	evaluation					2334:2343	the quality evaluation	2322:2343	the quality evaluation of DG materials	2322:2359	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	6	44	theme	Q-residual	1441:1450	arg1	values					1452:1457	Q-residual values	1441:1457	Q-residual values in a statistical quantitative sense	1441:1493	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	10	45	theme	E-nose	2127:2132	arg1	techniques					2146:2155	E-nose and GC-MS/O techniques	2127:2155	E-nose and GC-MS/O techniques	2127:2155	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	2	46	theme	substances	409:418	arg1	variety					389:395	a variety	387:395	a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others	387:513	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	46	theme	substances	409:418	arg1	substances					409:418	volatile substances	400:418	volatile substances	400:418	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	46	theme	substances	409:418	arg1	acids					446:450	organic acids	438:450	organic acids	438:450	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	46	theme	substances	409:418	arg1	polysaccharides					421:435	polysaccharides	421:435	polysaccharides	421:435	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	6	47	theme	sensor	1148:1153	arg1	responses					1155:1163	the E-nose sensor responses	1137:1163	the E-nose sensor responses	1137:1163	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	9	48	theme	flavour	1932:1938	arg1	more					1956:1959	more	1956:1959	more	1956:1959	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	9	48	theme	flavour	1932:1938	arg1	compounds					1940:1948	196 flavour compounds	1928:1948	196 flavour compounds	1928:1948	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	6	49	theme	analysis	1326:1333	arg1	model					1341:1345	a principal component analysis (PCA) model	1304:1345	a principal component analysis (PCA) model	1304:1345	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	8	50	theme	odour	1735:1739	arg1	attributes					1741:1750	the odour attributes	1731:1750	the odour attributes of DG herb	1731:1761	It was terpenes and aromatics of the flavour compounds that mainly contributed to the odour attributes of DG herb.
36740772	6	51	theme	principal	1306:1314	arg1	analysis					1326:1333	principal component analysis	1306:1333	a principal component analysis (PCA) model	1304:1345	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	6	51	theme	principal	1306:1314	arg1	PCA					1336:1338	PCA	1336:1338	PCA	1336:1338	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	10	52	theme	GC-MS/O	2138:2144	arg1	techniques					2146:2155	E-nose and GC-MS/O techniques	2127:2155	E-nose and GC-MS/O techniques	2127:2155	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	8	53	theme	herb	1758:1761	arg1	attributes					1741:1750	the odour attributes	1731:1750	the odour attributes of DG herb	1731:1761	It was terpenes and aromatics of the flavour compounds that mainly contributed to the odour attributes of DG herb.
36740772	6	54	theme	samples	1195:1201	arg1	batches					1181:1187	105 batches	1177:1187	105 batches of DG samples cultivated in six provinces of China	1177:1238	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	6	55	theme	sample	1382:1387	arg1	quality					1389:1395	the sample quality	1378:1395	the sample quality	1378:1395	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	2	56	theme	DG	363:364	arg1	material					366:373	DG material	363:373	DG material	363:373	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	5	57	theme	gas	840:842	arg1	GC × GC-TOF-MS					987:1000	GC × GC-TOF-MS	987:1000	GC × GC-TOF-MS	987:1000	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	57	theme	gas	840:842	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	57	theme	gas	840:842	arg1	GC-MS/O					891:897	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	GC-MS/O	891:897	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	11	58	theme	comprehensive	2295:2307	arg1	insight					2309:2315	a comprehensive insight	2293:2315	a comprehensive insight into the quality evaluation of DG materials	2293:2359	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	0	59	theme	comprehensive	166:178	arg1	spectrometry					235:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	5	60	theme	spectrometry/olfactometry	864:888	arg1	GC × GC-TOF-MS					987:1000	GC × GC-TOF-MS	987:1000	GC × GC-TOF-MS	987:1000	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	60	theme	spectrometry/olfactometry	864:888	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	60	theme	spectrometry/olfactometry	864:888	arg1	GC-MS/O					891:897	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	GC-MS/O	891:897	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	2	61	theme	edible	557:562	arg1	purposes					564:571	medicinal and edible purposes	543:571	medicinal and edible purposes in a long history	543:589	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	6	62	theme	odour	1276:1280	arg1	differentiations					1282:1297	their odour differentiations	1270:1297	their odour differentiations	1270:1297	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	11	63	dep	only	2165:2168	arg1	not					2161:2163	not	2161:2163	not	2161:2163	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	0	64	theme	gas	196:198	arg1	spectrometry					235:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	166:246	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	5	65	theme	DG	1109:1110	arg1	materials					1112:1120	DG materials	1109:1120	DG materials	1109:1120	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	2	66	theme	long	578:581	arg1	history					583:589	a long history	576:589	a long history	576:589	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	4	67	theme	materials	736:744	arg1	compositions					717:728	volatile flavour compositions	700:728	volatile flavour compositions of DG materials	700:744	OBJECTIVES This study was to determine volatile flavour compositions of DG materials and to reveal the odour complexity.
36740772	0	68	theme	sinensis	72:79	arg1	identification					17:30	Volatile flavour identification	0:30	Volatile flavour identification	0:30	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	0	68	theme	sinensis	72:79	arg1	complexity					42:51	odour complexity	36:51	odour complexity	36:51	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	6	69	from	values	1452:1457	arg1	sense					1489:1493	a statistical quantitative sense	1462:1493	a statistical quantitative sense	1462:1493	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	5	70	theme	two-dimensional	918:932	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	70	theme	two-dimensional	918:932	arg1	spectrometry					973:984	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	spectrometry	973:984	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	6	71	theme	China	1234:1238	arg1	provinces					1221:1229	six provinces	1217:1229	six provinces of China	1217:1238	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	0	72	theme	electronic	84:93	arg1	nose					95:98	electronic nose	84:98	electronic nose	84:98	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	5	73	theme	chromatography-time-of-flight-mass	938:971	arg1	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	73	theme	chromatography-time-of-flight-mass	938:971	arg1	spectrometry					973:984	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	spectrometry	973:984	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	11	74	theme	materials	2351:2359	arg1	evaluation					2334:2343	the quality evaluation	2322:2343	the quality evaluation of DG materials	2322:2359	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	4	75	theme	flavour	709:715	arg1	compositions					717:728	volatile flavour compositions	700:728	volatile flavour compositions of DG materials	700:744	OBJECTIVES This study was to determine volatile flavour compositions of DG materials and to reveal the odour complexity.
36740772	7	76	theme	odour-active	1591:1602	arg1	areas					1604:1608	51 odour-active areas	1588:1608	51 odour-active areas discerned	1588:1618	By the GC-MS/O and GC × GC-TOF-MS analyses, 196 volatile flavour compounds were identified, 51 odour-active areas discerned and 39 odourants determined.
36740772	2	77	theme	medicinal	543:551	arg1	purposes					564:571	medicinal and edible purposes	543:571	medicinal and edible purposes in a long history	543:589	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	0	78	theme	gas	112:114	arg1	spectrometry/olfactometry					136:160	integrated gas chromatography-mass spectrometry/olfactometry	101:160	integrated gas chromatography-mass spectrometry/olfactometry	101:160	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	6	79	theme	E-nose	1141:1146	arg1	responses					1155:1163	the E-nose sensor responses	1137:1163	the E-nose sensor responses	1137:1163	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	11	80	theme	sensory	2260:2266	arg1	properties					2268:2277	sensory properties	2260:2277	sensory properties	2260:2277	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	9	81	dep	CONCLUSION	1764:1773	arg1	made					1893:1896	made	1893:1896	made a breakthrough in determining 196 flavour compounds, much more than those in any previous report	1893:1993	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	9	81	dep	CONCLUSION	1764:1773	arg1	time					1820:1823	the first time	1810:1823	the first time employed to analyse the volatile flavours of DG materials	1810:1881	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	9	81	dep	CONCLUSION	1764:1773	arg1	method					1799:1804	The SPME-GC × GC-TOF-MS method	1775:1804	The SPME-GC × GC-TOF-MS method	1775:1804	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	0	82	theme	flavour	9:15	arg1	identification					17:30	Volatile flavour identification	0:30	Volatile flavour identification	0:30	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	6	83	theme	statistical	1464:1474	arg1	sense					1489:1493	a statistical quantitative sense	1462:1493	a statistical quantitative sense	1462:1493	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	1	84	theme	traditional	332:342	arg1	medicines					352:360	the typical traditional Chinese medicines	320:360	the typical traditional Chinese medicines	320:360	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	5	85	theme	solid-phase	1018:1028	arg1	SPME					1048:1051	SPME	1048:1051	SPME	1048:1051	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	85	theme	solid-phase	1018:1028	arg1	micro-extraction					1030:1045	solid-phase micro-extraction	1018:1045	solid-phase micro-extraction (SPME)	1018:1052	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	9	86	theme	DG	1870:1871	arg1	materials					1873:1881	DG materials	1870:1881	DG materials	1870:1881	CONCLUSION The SPME-GC × GC-TOF-MS method was the first time employed to analyse the volatile flavours of DG materials, and thus made a breakthrough in determining 196 flavour compounds, much more than those in any previous report.
36740772	2	87	used	used	534:537	arg2	material					366:373	DG material	363:373	DG material	363:373	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	88	theme	amino	464:468	arg1	acids					446:450	organic acids	438:450	organic acids	438:450	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	2	88	theme	amino	464:468	arg1	acids					470:474	amino acids	464:474	amino acids	464:474	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	1	89	theme	medicines	352:360	arg1	one					313:315	one	313:315	one	313:315	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	1	89	theme	medicines	352:360	arg1	medicines					352:360	the typical traditional Chinese medicines	320:360	the typical traditional Chinese medicines	320:360	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	1	89	theme	medicines	352:360	arg1	INTRODUCTION					249:260	INTRODUCTION Radix Angelicae sinensis (Danggui, DG)	249:299	INTRODUCTION Radix Angelicae sinensis (Danggui, DG)	249:299	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	4	90	dep	OBJECTIVES	661:670	arg1	was					683:685	was	683:685	was to determine volatile flavour compositions of DG materials and to reveal the odour complexity	683:779	OBJECTIVES This study was to determine volatile flavour compositions of DG materials and to reveal the odour complexity.
36740772	11	91	dep	compositions	2244:2255	arg1	any					2226:2228	any	2226:2228	any	2226:2228	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	3	92	theme	material	643:650	arg1	quality					652:658	the DG material quality	636:658	the DG material quality	636:658	The fragrance is of importance to assessing the DG material quality.
36740772	0	93	theme	radix	56:60	arg1	sinensis					72:79	radix Angelicae sinensis	56:79	radix Angelicae sinensis	56:79	Volatile flavour identification and odour complexity of radix Angelicae sinensis by electronic nose, integrated gas chromatography-mass spectrometry/olfactometry and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry.
36740772	5	94	theme	flavour	1087:1093	arg1	complexity					1095:1104	the flavour complexity	1083:1104	the flavour complexity of DG materials	1083:1120	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	6	95	theme	batches	1181:1187	arg1	total					1168:1172	a total	1166:1172	a total of 105 batches of DG samples cultivated in six provinces of China	1166:1238	RESULTS Using the E-nose sensor responses, a total of 105 batches of DG samples cultivated in six provinces of China were categorised according to their odour differentiations, and a principal component analysis (PCA) model was established for evaluating the sample quality through a combination of Hotelling's T2 and Q-residual values in a statistical quantitative sense.
36740772	1	96	dep	INTRODUCTION	249:260	arg1	DG					297:298	DG	297:298	DG	297:298	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	1	96	dep	INTRODUCTION	249:260	arg1	sinensis					278:285	Radix Angelicae sinensis	262:285	INTRODUCTION Radix Angelicae sinensis (Danggui, DG)	249:299	INTRODUCTION Radix Angelicae sinensis (Danggui, DG) is known as one of the typical traditional Chinese medicines.
36740772	5	97	used	used	1067:1070	arg2	METHODS					795:801	METHODS Electronic nose (E-nose)	795:826	METHODS Electronic nose (E-nose)	795:826	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	97	used	used	1067:1070	arg2	MATERIAL					782:789	MATERIAL	782:789	MATERIAL	782:789	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	97	used	used	1067:1070	arg2	spectrometry					973:984	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	spectrometry	973:984	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	5	97	used	used	1067:1070	arg2	GC-MS/O					891:897	integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry	829:984	GC-MS/O	891:897	MATERIAL AND METHODS Electronic nose (E-nose), integrated gas chromatography-mass spectrometry/olfactometry (GC-MS/O) and comprehensive two-dimensional gas chromatography-time-of-flight-mass spectrometry (GC × GC-TOF-MS), combined with solid-phase micro-extraction (SPME), were mainly used to address the flavour complexity of DG materials.
36740772	11	98	theme	DG	2348:2349	arg1	materials					2351:2359	DG materials	2348:2359	DG materials	2348:2359	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
36740772	2	99	from	purposes	564:571	arg1	history					583:589	a long history	576:589	a long history	576:589	DG material consists of a variety of volatile substances, polysaccharides, organic acids, ceramides, amino acids, vitamins, microelements, among others, and thus has been used for medicinal and edible purposes in a long history.
36740772	7	100	theme	volatile	1544:1551	arg1	compounds					1561:1569	196 volatile flavour compounds	1540:1569	196 volatile flavour compounds	1540:1569	By the GC-MS/O and GC × GC-TOF-MS analyses, 196 volatile flavour compounds were identified, 51 odour-active areas discerned and 39 odourants determined.
36740772	10	101	theme	odour	2079:2083	arg1	complexity					2085:2094	odour complexity	2079:2094	odour complexity	2079:2094	The work also made a significant step forward to link the flavour compositions and odour complexity of radix Angelicae sinensis by E-nose and GC-MS/O techniques.
36740772	11	102	theme	PCA	2193:2195	arg1	model					2197:2201	a statistical PCA model	2179:2201	a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials	2179:2359	It not only provided a statistical PCA model that did not depend on any predetermined compositions or sensory properties for, but also a comprehensive insight into the quality evaluation of DG materials.
35345496	5	0	theme	species	760:766	arg1	extract					736:742	the extract	732:742	the extract of Enteromorpha species	732:766	It was found that the extract of Enteromorpha species contains the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml).
35345496	4	1	theme	Enteromorpha	488:499	arg1	species					501:507	Enteromorpha species	488:507	Enteromorpha species	488:507	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	4	2	theme	MTT	703:705	arg1	assay					707:711	MTT assay	703:711	MTT assay	703:711	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	4	3	theme	antioxidant	582:592	arg1	activity					594:601	total antioxidant activity	576:601	total antioxidant activity by phosphomolybdenum assay	576:628	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	8	4	theme	prepared	1163:1170	arg1	extract					1184:1190	The prepared green algal extract	1159:1190	The prepared green algal extract	1159:1190	The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.
35345496	2	5	theme	polysaccharides	336:350	arg1	presence					315:322	the presence	311:322	the presence of sulfated polysaccharides (SPs)	311:356	The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs).
35345496	4	6	theme	total	576:580	arg1	activity					594:601	total antioxidant activity	576:601	total antioxidant activity by phosphomolybdenum assay	576:628	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	0	7	theme	in	91:92	arg1	antioxidant					100:110	in vitro antioxidant	91:110	in vitro antioxidant	91:110	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	7	8	theme	antioxidant	1079:1089	arg1	assay					1091:1095	the total antioxidant assay	1069:1095	the total antioxidant assay (400 µl/ml)	1069:1107	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	7	8	theme	antioxidant	1079:1089	arg1	400 µl/ml					1098:1106	400 µl/ml	1098:1106	400 µl/ml	1098:1106	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	2	9	theme	high	277:280	arg1	potential					294:302	a high antioxidant potential	275:302	a high antioxidant potential	275:302	The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs).
35345496	2	10	theme	antioxidant	282:292	arg1	potential					294:302	a high antioxidant potential	275:302	a high antioxidant potential	275:302	The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs).
35345496	8	11	dep	18 mm	1235:1239	arg1	up					1229:1230	up	1229:1230	up	1229:1230	The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.
35345496	6	12	located	found	980:984	arg2	Phytochemicals					918:931	Phytochemicals	918:931	Phytochemicals such as flavonoids and phenolic compounds	918:973	Phytochemicals such as flavonoids and phenolic compounds were found in the extract.
35345496	6	12	located	found	980:984	arg2	flavonoids					941:950	flavonoids	941:950	flavonoids	941:950	Phytochemicals such as flavonoids and phenolic compounds were found in the extract.
35345496	6	12	located	found	980:984	arg1	extract					993:999	the extract	989:999	the extract	989:999	Phytochemicals such as flavonoids and phenolic compounds were found in the extract.
35345496	6	12	located	found	980:984	arg2	compounds					965:973	phenolic compounds	956:973	phenolic compounds	956:973	Phytochemicals such as flavonoids and phenolic compounds were found in the extract.
35345496	6	13	theme	phenolic	956:963	arg1	compounds					965:973	phenolic compounds	956:973	phenolic compounds	956:973	Phytochemicals such as flavonoids and phenolic compounds were found in the extract.
35345496	0	14	theme	antioxidant	100:110	arg1	determination					74:86	the determination	70:86	the determination of in vitro antioxidant and antibacterial activities	70:139	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	9	15	theme	antioxidant	1463:1473	arg1	activities					1494:1503	strong antioxidant and anti-bacterial activities	1456:1503	strong antioxidant and anti-bacterial activities	1456:1503	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	9	16	theme	species	1438:1444	arg1	extract					1414:1420	the extract	1410:1420	the extract of Enteromorpha species	1410:1444	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	0	17	dep	in	91:92	arg1	vitro					94:98	vitro	94:98	vitro	94:98	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	1	18	theme	algae	163:167	arg1	extract					146:152	The extract	142:152	The extract of green algae (Enteromorpha species)	142:190	The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique.
35345496	7	19	theme	total	1073:1077	arg1	assay					1091:1095	the total antioxidant assay	1069:1095	the total antioxidant assay (400 µl/ml)	1069:1107	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	7	19	theme	total	1073:1077	arg1	400 µl/ml					1098:1106	400 µl/ml	1098:1106	400 µl/ml	1098:1106	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	4	20	theme	phosphomolybdenum	606:622	arg1	assay					624:628	phosphomolybdenum assay	606:628	phosphomolybdenum assay	606:628	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	4	21	theme	diffusion	663:671	arg1	method					673:678	agar diffusion method	658:678	agar diffusion method	658:678	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	1	22	theme	Enteromorpha	170:181	arg1	algae					163:167	green algae	157:167	green algae (Enteromorpha species)	157:190	The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique.
35345496	1	22	theme	Enteromorpha	170:181	arg1	species					183:189	Enteromorpha species	170:189	Enteromorpha species	170:189	The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique.
35345496	4	23	theme	scavenging	555:564	arg1	activity					566:573	the DPPH-free radical scavenging activity	533:573	the DPPH-free radical scavenging activity	533:573	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	8	24	theme	highest	1205:1211	arg1	zone					1224:1227	the highest inhibitory zone	1201:1227	the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm	1201:1257	The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.
35345496	7	25	theme	crude	1035:1039	arg1	extract					1041:1047	the crude extract	1031:1047	the crude extract	1031:1047	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	8	26	theme	inhibitory	1213:1222	arg1	zone					1224:1227	the highest inhibitory zone	1201:1227	the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm	1201:1257	The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.
35345496	0	27	theme	species	26:32	arg1	Isolation					0:8	Isolation	0:8	Isolation of Enteromorpha species	0:32	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	4	28	theme	radical	547:553	arg1	activity					566:573	the DPPH-free radical scavenging activity	533:573	the DPPH-free radical scavenging activity	533:573	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	0	29	theme	antibacterial	116:128	arg1	activities					130:139	antibacterial activities	116:139	antibacterial activities	116:139	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	5	30	theme	chemical	789:796	arg1	composition					798:808	the various chemical composition	777:808	the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml)	777:915	It was found that the extract of Enteromorpha species contains the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml).
35345496	3	31	theme	biochemical	448:458	arg1	composition					460:470	significant biochemical composition	436:470	significant biochemical composition	436:470	The extract of Enteromorpha species was analyzed to identify the presence of significant biochemical composition.
35345496	0	32	theme	Enteromorpha	13:24	arg1	species					26:32	Enteromorpha species	13:32	Enteromorpha species	13:32	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	9	33	theme	polysaccharides	1537:1551	arg1	presence					1516:1523	the presence	1512:1523	the presence of sulfated polysaccharides	1512:1551	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	1	34	theme	green	157:161	arg1	algae					163:167	green algae	157:167	green algae (Enteromorpha species)	157:190	The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique.
35345496	1	34	theme	green	157:161	arg1	species					183:189	Enteromorpha species	170:189	Enteromorpha species	170:189	The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique.
35345496	7	35	theme	radical	1123:1129	arg1	assay					1142:1146	DPPH-free radical scavenging assay	1113:1146	DPPH-free radical scavenging assay (5 µl/ml)	1113:1156	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	7	35	theme	radical	1123:1129	arg1	5 µl/ml					1149:1155	5 µl/ml	1149:1155	5 µl/ml	1149:1155	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	3	36	theme	composition	460:470	arg1	presence					424:431	the presence	420:431	the presence of significant biochemical composition	420:470	The extract of Enteromorpha species was analyzed to identify the presence of significant biochemical composition.
35345496	7	37	theme	DPPH-free	1113:1121	arg1	assay					1142:1146	DPPH-free radical scavenging assay	1113:1146	DPPH-free radical scavenging assay (5 µl/ml)	1113:1156	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	7	37	theme	DPPH-free	1113:1121	arg1	5 µl/ml					1149:1155	5 µl/ml	1149:1155	5 µl/ml	1149:1155	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	4	38	theme	agar	658:661	arg1	method					673:678	agar diffusion method	658:678	agar diffusion method	658:678	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	5	39	theme	various	781:787	arg1	composition					798:808	the various chemical composition	777:808	the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml)	777:915	It was found that the extract of Enteromorpha species contains the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml).
35345496	0	40	theme	activities	130:139	arg1	determination					74:86	the determination	70:86	the determination of in vitro antioxidant and antibacterial activities	70:139	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	7	41	theme	scavenging	1131:1140	arg1	assay					1142:1146	DPPH-free radical scavenging assay	1113:1146	DPPH-free radical scavenging assay (5 µl/ml)	1113:1156	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	7	41	theme	scavenging	1131:1140	arg1	5 µl/ml					1149:1155	5 µl/ml	1149:1155	5 µl/ml	1149:1155	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	3	42	theme	significant	436:446	arg1	composition					460:470	significant biochemical composition	436:470	significant biochemical composition	436:470	The extract of Enteromorpha species was analyzed to identify the presence of significant biochemical composition.
35345496	0	43	theme	crude	52:56	arg1	extract					58:64	its crude extract	48:64	its crude extract	48:64	Isolation of Enteromorpha species and analyzing its crude extract for the determination of in vitro antioxidant and antibacterial activities.
35345496	7	44	theme	extract	1041:1047	arg1	potential					1018:1026	The antioxidant potential	1002:1026	The antioxidant potential of the crude extract	1002:1047	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	8	45	theme	green	1172:1176	arg1	extract					1184:1190	The prepared green algal extract	1159:1190	The prepared green algal extract	1159:1190	The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.
35345496	5	46	theme	Enteromorpha	747:758	arg1	species					760:766	Enteromorpha species	747:766	Enteromorpha species	747:766	It was found that the extract of Enteromorpha species contains the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml).
35345496	2	47	theme	sulfated	327:334	arg1	SPs					353:355	SPs	353:355	SPs	353:355	The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs).
35345496	2	47	theme	sulfated	327:334	arg1	polysaccharides					336:350	sulfated polysaccharides	327:350	sulfated polysaccharides (SPs)	327:356	The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs).
35345496	3	48	theme	species	387:393	arg1	extract					363:369	The extract	359:369	The extract of Enteromorpha species	359:393	The extract of Enteromorpha species was analyzed to identify the presence of significant biochemical composition.
35345496	4	49	theme	DPPH-free	537:545	arg1	activity					566:573	the DPPH-free radical scavenging activity	533:573	the DPPH-free radical scavenging activity	533:573	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	5	50	contain	contains	768:775	arg1	extract					736:742	the extract	732:742	the extract of Enteromorpha species	732:766	It was found that the extract of Enteromorpha species contains the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml).
35345496	5	50	contain	contains	768:775	arg2	composition					798:808	the various chemical composition	777:808	the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml)	777:915	It was found that the extract of Enteromorpha species contains the various chemical composition such as carbohydrates (0.13 g/ml), xylose (0.0819 g/ml), sulfate (0.0153 g/ml), and proteins (0.0363 g/ml).
35345496	8	51	theme	algal	1178:1182	arg1	extract					1184:1190	The prepared green algal extract	1159:1190	The prepared green algal extract	1159:1190	The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.
35345496	9	52	theme	sulfated	1528:1535	arg1	polysaccharides					1537:1551	sulfated polysaccharides	1528:1551	sulfated polysaccharides	1528:1551	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	2	53	theme	algal	252:256	arg1	extract					258:264	The prepared algal extract	239:264	The prepared algal extract	239:264	The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs).
35345496	9	54	theme	anti-bacterial	1479:1492	arg1	activities					1494:1503	strong antioxidant and anti-bacterial activities	1456:1503	strong antioxidant and anti-bacterial activities	1456:1503	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	7	55	theme	antioxidant	1006:1016	arg1	potential					1018:1026	The antioxidant potential	1002:1026	The antioxidant potential of the crude extract	1002:1047	The antioxidant potential of the crude extract was investigated by the total antioxidant assay (400 µl/ml) and DPPH-free radical scavenging assay (5 µl/ml).
35345496	9	56	theme	above	1382:1386	arg1	results					1388:1394	The above results	1378:1394	The above results	1378:1394	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	2	57	theme	prepared	243:250	arg1	extract					258:264	The prepared algal extract	239:264	The prepared algal extract	239:264	The prepared algal extract exhibits a high antioxidant potential due to the presence of sulfated polysaccharides (SPs).
35345496	1	58	theme	cold	212:215	arg1	technique					228:236	the cold extraction technique	208:236	the cold extraction technique	208:236	The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique.
35345496	9	59	theme	strong	1456:1461	arg1	activities					1494:1503	strong antioxidant and anti-bacterial activities	1456:1503	strong antioxidant and anti-bacterial activities	1456:1503	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	9	60	theme	Enteromorpha	1425:1436	arg1	species					1438:1444	Enteromorpha species	1425:1444	Enteromorpha species	1425:1444	The above results revealed that the extract of Enteromorpha species exhibited strong antioxidant and anti-bacterial activities due to the presence of sulfated polysaccharides.
35345496	8	61	theme	200 µl/ml	1262:1270	arg1	concentrations					1272:1285	200 µl/ml concentrations	1262:1285	200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli	1262:1361	The prepared green algal extract produced the highest inhibitory zone up to 18 mm, 13 mm, and 18 mm at 200 µl/ml concentrations against Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli, respectively.
35345496	1	62	theme	extraction	217:226	arg1	technique					228:236	the cold extraction technique	208:236	the cold extraction technique	208:236	The extract of green algae (Enteromorpha species) was prepared by the cold extraction technique.
35345496	3	63	theme	Enteromorpha	374:385	arg1	species					387:393	Enteromorpha species	374:393	Enteromorpha species	374:393	The extract of Enteromorpha species was analyzed to identify the presence of significant biochemical composition.
35345496	4	64	theme	species	501:507	arg1	extract					477:483	The extract	473:483	The extract of Enteromorpha species	473:507	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
35345496	4	65	theme	cell	685:688	arg1	viability					690:698	cell viability	685:698	cell viability	685:698	The extract of Enteromorpha species was evaluated to assess the DPPH-free radical scavenging activity, total antioxidant activity by phosphomolybdenum assay, in vitro anti-bacterial by agar diffusion method, and cell viability by MTT assay.
37283513	1	0	theme	due	338:340	arg1	attention					328:336	attention	328:336	attention due to its potential applications toward efficient and safe antibiotics	328:408	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	3	1	theme	double	490:495	arg1	effect					509:514	the double cooperative effect	486:514	the double cooperative effect	486:514	In this work, we report that the double cooperative effect can be partially recapitulated in synthetic lipid systems just by varying the lipid composition between eukaryotic and Escherichia coli membranes.
37283513	3	2	theme	cooperative	497:507	arg1	effect					509:514	the double cooperative effect	486:514	the double cooperative effect	486:514	In this work, we report that the double cooperative effect can be partially recapitulated in synthetic lipid systems just by varying the lipid composition between eukaryotic and Escherichia coli membranes.
37283513	1	3	theme	host	257:260	arg1	damage					262:267	the host damage	253:267	the host damage	253:267	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	0	4	from	Role	0:3	arg1	Effect					74:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.
37283513	4	5	theme	cooperative	861:871	arg1	effect					873:878	the double cooperative effect	850:878	the double cooperative effect	850:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	6	theme	membrane	752:759	arg1	proteins					761:768	membrane proteins	752:768	membrane proteins	752:768	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	3	7	theme	lipid	560:564	arg1	systems					566:572	synthetic lipid systems	550:572	synthetic lipid systems	550:572	In this work, we report that the double cooperative effect can be partially recapitulated in synthetic lipid systems just by varying the lipid composition between eukaryotic and Escherichia coli membranes.
37283513	1	8	theme	potential	349:357	arg1	applications					359:370	its potential applications	345:370	its potential applications toward efficient and safe antibiotics	345:408	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	4	9	theme	double	854:859	arg1	effect					873:878	the double cooperative effect	850:878	the double cooperative effect	850:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	10	theme	main	827:830	arg1	forces					840:845	the main driving forces	823:845	the main driving forces of the double cooperative effect	823:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	1	11	theme	major	160:164	arg1	peptides					180:187	two major antimicrobial peptides	156:187	two major antimicrobial peptides LL-37 and HNP1	156:202	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	11	theme	major	160:164	arg1	HNP1					199:202	HNP1	199:202	HNP1	199:202	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	11	theme	major	160:164	arg1	LL-37					189:193	LL-37	189:193	LL-37	189:193	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	12	theme	antimicrobial	166:178	arg1	peptides					180:187	two major antimicrobial peptides	156:187	two major antimicrobial peptides LL-37 and HNP1	156:202	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	12	theme	antimicrobial	166:178	arg1	HNP1					199:202	HNP1	199:202	HNP1	199:202	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	12	theme	antimicrobial	166:178	arg1	LL-37					189:193	LL-37	189:193	LL-37	189:193	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	13	theme	efficient	379:387	arg1	antibiotics					398:408	efficient and safe antibiotics	379:408	efficient and safe antibiotics	379:408	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	4	14	theme	forces	840:845	arg1	interaction					906:916	a simple lipid-peptide interaction	883:916	a simple lipid-peptide interaction	883:916	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	14	theme	forces	840:845	arg1	forces					840:845	the main driving forces	823:845	the main driving forces of the double cooperative effect	823:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	14	theme	forces	840:845	arg1	one					816:818	one	816:818	one	816:818	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	1	15	theme	peptides	180:187	arg1	mixture					145:151	the mixture	141:151	the mixture of two major antimicrobial peptides LL-37 and HNP1	141:202	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	4	16	theme	driving	832:838	arg1	forces					840:845	the main driving forces	823:845	the main driving forces of the double cooperative effect	823:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	1	17	theme	antimicrobial	86:98	arg1	effect					127:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	4	18	theme	cell	677:680	arg1	membranes					682:690	real cell membranes	672:690	real cell membranes	672:690	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	0	19	theme	Composition	14:24	arg1	Role					0:3	Role	0:3	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.	0:80	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.
37283513	1	20	theme	mammalian	284:292	arg1	lysis					308:312	mammalian cell membrane lysis	284:312	mammalian cell membrane lysis	284:312	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	21	theme	peptide	100:106	arg1	effect					127:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	0	22	theme	Lipid	8:12	arg1	Composition					14:24	Lipid Composition	8:24	Lipid Composition	8:24	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.
37283513	1	23	theme	cell	294:297	arg1	lysis					308:312	mammalian cell membrane lysis	284:312	mammalian cell membrane lysis	284:312	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	4	24	theme	lipid-peptide	892:904	arg1	interaction					906:916	a simple lipid-peptide interaction	883:916	a simple lipid-peptide interaction	883:916	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	24	theme	lipid-peptide	892:904	arg1	forces					840:845	the main driving forces	823:845	the main driving forces of the double cooperative effect	823:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	24	theme	lipid-peptide	892:904	arg1	one					816:818	one	816:818	one	816:818	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	1	25	theme	double	108:113	arg1	effect					127:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	26	theme	membrane	299:306	arg1	lysis					308:312	mammalian cell membrane lysis	284:312	mammalian cell membrane lysis	284:312	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	27	theme	safe	393:396	arg1	antibiotics					398:408	efficient and safe antibiotics	379:408	efficient and safe antibiotics	379:408	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	28	dep	peptides	180:187	arg1	peptides					180:187	two major antimicrobial peptides	156:187	two major antimicrobial peptides LL-37 and HNP1	156:202	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	28	dep	peptides	180:187	arg1	HNP1					199:202	HNP1	199:202	HNP1	199:202	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	1	28	dep	peptides	180:187	arg1	LL-37					189:193	LL-37	189:193	LL-37	189:193	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	4	29	theme	real	672:675	arg1	membranes					682:690	real cell membranes	672:690	real cell membranes	672:690	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	3	30	theme	Escherichia	635:645	arg1	membranes					652:660	Escherichia coli membranes	635:660	Escherichia coli membranes	635:660	In this work, we report that the double cooperative effect can be partially recapitulated in synthetic lipid systems just by varying the lipid composition between eukaryotic and Escherichia coli membranes.
37283513	3	31	theme	lipid	594:598	arg1	composition					600:610	the lipid composition	590:610	the lipid composition between eukaryotic and Escherichia coli membranes	590:660	In this work, we report that the double cooperative effect can be partially recapitulated in synthetic lipid systems just by varying the lipid composition between eukaryotic and Escherichia coli membranes.
37283513	0	32	theme	Peptide	47:53	arg1	Effect					74:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.
37283513	1	33	theme	cooperative	115:125	arg1	effect					127:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect	82:132	The antimicrobial peptide double cooperative effect, where the mixture of two major antimicrobial peptides LL-37 and HNP1 kills bacteria more efficiently while minimizing the host damage by suppressing mammalian cell membrane lysis, has garnered attention due to its potential applications toward efficient and safe antibiotics.
37283513	0	34	theme	Antimicrobial	33:45	arg1	Effect					74:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.
37283513	4	35	theme	effect	873:878	arg1	forces					840:845	the main driving forces	823:845	the main driving forces of the double cooperative effect	823:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	36	theme	simple	885:890	arg1	interaction					906:916	a simple lipid-peptide interaction	883:916	a simple lipid-peptide interaction	883:916	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	36	theme	simple	885:890	arg1	forces					840:845	the main driving forces	823:845	the main driving forces of the double cooperative effect	823:878	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	4	36	theme	simple	885:890	arg1	one					816:818	one	816:818	one	816:818	Although real cell membranes are so much more complex than just lipids, including, e.g., membrane proteins and polysaccharides, our data implicates that one of the main driving forces of the double cooperative effect is a simple lipid-peptide interaction.
37283513	3	37	theme	synthetic	550:558	arg1	systems					566:572	synthetic lipid systems	550:572	synthetic lipid systems	550:572	In this work, we report that the double cooperative effect can be partially recapitulated in synthetic lipid systems just by varying the lipid composition between eukaryotic and Escherichia coli membranes.
37283513	3	38	dep	Escherichia	635:645	arg1	coli					647:650	coli	647:650	coli	647:650	In this work, we report that the double cooperative effect can be partially recapitulated in synthetic lipid systems just by varying the lipid composition between eukaryotic and Escherichia coli membranes.
37283513	0	39	theme	Cooperative	62:72	arg1	Effect					74:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.
37283513	0	40	theme	Double	55:60	arg1	Effect					74:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	the Antimicrobial Peptide Double Cooperative Effect	29:79	Role of Lipid Composition in the Antimicrobial Peptide Double Cooperative Effect.
37374879	3	0	theme	Cell	317:320	arg1	modification					327:338	Cell wall modification	317:338	Cell wall modification	317:338	Cell wall modification is one of the defense strategies that helps bacteria escape from recognition and/or internalization by its predators.
37374879	8	1	theme	significant	1040:1050	arg1	role					1052:1055	a significant role	1038:1055	a significant role	1038:1055	We found that length of O-antigen does not play a significant role in regulating bacterial recognition by A. castellanii.
37374879	1	2	theme	bacterial	149:157	arg1	size					170:173	bacterial population size	149:173	bacterial population size	149:173	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	7	3	dep	E.	931:932	arg1	coli					934:937	coli	934:937	coli	934:937	Here, we investigate how length, structure and composition of LPS affect E. coli recognition and internalization by A. castellanii.
37374879	3	4	theme	wall	322:325	arg1	modification					327:338	Cell wall modification	317:338	Cell wall modification	317:338	Cell wall modification is one of the defense strategies that helps bacteria escape from recognition and/or internalization by its predators.
37374879	4	5	theme	bacterial	523:531	arg1	wall					538:541	Gram-negative bacterial cell wall	509:541	Gram-negative bacterial cell wall	509:541	Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall.
37374879	6	6	dep	E.	684:685	arg1	coli					687:690	coli	687:690	coli	687:690	O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown.
37374879	1	7	theme	population	159:168	arg1	size					170:173	bacterial population size	149:173	bacterial population size	149:173	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	5	8	dep	regions	570:576	arg1	regions					570:576	three regions	564:576	three regions: lipid A, oligosaccharide core and O-specific polysaccharide	564:637	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	5	8	dep	regions	570:576	arg1	A					585:585	lipid A	579:585	lipid A	579:585	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	5	8	dep	regions	570:576	arg1	core					604:607	oligosaccharide core	588:607	oligosaccharide core	588:607	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	5	8	dep	regions	570:576	arg1	polysaccharide					624:637	O-specific polysaccharide	613:637	O-specific polysaccharide	613:637	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	4	9	theme	Gram-negative	509:521	arg1	wall					538:541	Gram-negative bacterial cell wall	509:541	Gram-negative bacterial cell wall	509:541	Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall.
37374879	5	10	theme	oligosaccharide	588:602	arg1	regions					570:576	three regions	564:576	three regions: lipid A, oligosaccharide core and O-specific polysaccharide	564:637	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	5	10	theme	oligosaccharide	588:602	arg1	core					604:607	oligosaccharide core	588:607	oligosaccharide core	588:607	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	3	11	theme	defense	354:360	arg1	strategies					362:371	the defense strategies	350:371	the defense strategies	350:371	Cell wall modification is one of the defense strategies that helps bacteria escape from recognition and/or internalization by its predators.
37374879	7	12	theme	A.	974:975	arg1	castellanii					977:987	A. castellanii	974:987	A. castellanii	974:987	Here, we investigate how length, structure and composition of LPS affect E. coli recognition and internalization by A. castellanii.
37374879	5	13	theme	O-specific	613:622	arg1	regions					570:576	three regions	564:576	three regions: lipid A, oligosaccharide core and O-specific polysaccharide	564:637	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	5	13	theme	O-specific	613:622	arg1	polysaccharide					624:637	O-specific polysaccharide	613:637	O-specific polysaccharide	613:637	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	9	14	theme	castellanii	1222:1232	arg1	predation					1234:1242	A. castellanii predation	1219:1242	A. castellanii predation	1219:1242	However, the composition and structure of O-polysaccharide play important roles in providing resistance to A. castellanii predation.
37374879	9	15	dep	composition	1125:1135	arg1	the					1121:1123	the	1121:1123	the	1121:1123	However, the composition and structure of O-polysaccharide play important roles in providing resistance to A. castellanii predation.
37374879	4	16	theme	major	490:494	arg1	Lipopolysaccharide					458:475	Lipopolysaccharide	458:475	Lipopolysaccharide (LPS)	458:481	Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall.
37374879	4	16	theme	major	490:494	arg1	component					496:504	the major component	486:504	the major component of Gram-negative bacterial cell wall	486:541	Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall.
37374879	2	17	theme	defense	268:274	arg1	strategies					276:285	many defense strategies	263:285	many defense strategies	263:285	To enhance their survival, bacteria evolved many defense strategies to avoid grazing by protists.
37374879	8	18	theme	O-antigen	1014:1022	arg1	length					1004:1009	length	1004:1009	length of O-antigen	1004:1022	We found that length of O-antigen does not play a significant role in regulating bacterial recognition by A. castellanii.
37374879	6	19	dep	contribute	812:821	arg1	remain					842:847	remain	842:847	remain unknown	842:855	O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown.
37374879	5	20	theme	lipid	579:583	arg1	regions					570:576	three regions	564:576	three regions: lipid A, oligosaccharide core and O-specific polysaccharide	564:637	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	5	20	theme	lipid	579:583	arg1	A					585:585	lipid A	579:585	lipid A	579:585	LPS is divided into three regions: lipid A, oligosaccharide core and O-specific polysaccharide.
37374879	8	21	theme	bacterial	1071:1079	arg1	recognition					1081:1091	bacterial recognition	1071:1091	bacterial recognition by A. castellanii	1071:1109	We found that length of O-antigen does not play a significant role in regulating bacterial recognition by A. castellanii.
37374879	6	22	theme	LPS	692:694	arg1	region					674:679	the outermost region	660:679	the outermost region of E. coli LPS	660:694	O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown.
37374879	3	23	theme	strategies	362:371	arg1	strategies					362:371	the defense strategies	350:371	the defense strategies	350:371	Cell wall modification is one of the defense strategies that helps bacteria escape from recognition and/or internalization by its predators.
37374879	3	23	theme	strategies	362:371	arg1	one					343:345	one	343:345	one	343:345	Cell wall modification is one of the defense strategies that helps bacteria escape from recognition and/or internalization by its predators.
37374879	6	24	theme	O-polysaccharide	795:810	arg1	characteristics					776:790	the characteristics	772:790	the characteristics of O-polysaccharide	772:810	O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown.
37374879	0	25	theme	LPS	21:23	arg1	O-Polysaccharides					0:16	O-Polysaccharides	0:16	O-Polysaccharides of LPS	0:23	O-Polysaccharides of LPS Modulate E. coli Uptake by Acanthamoeba castellanii.
37374879	8	26	theme	A.	1096:1097	arg1	castellanii					1099:1109	A. castellanii	1096:1109	A. castellanii	1096:1109	We found that length of O-antigen does not play a significant role in regulating bacterial recognition by A. castellanii.
37374879	1	27	theme	major	101:105	arg1	cause					107:111	a major cause	99:111	a major cause of bacterial mortality	99:134	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	1	27	theme	major	101:105	arg1	grazing					88:94	Protozoan grazing	78:94	Protozoan grazing	78:94	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	6	28	theme	E.	684:685	arg1	LPS					692:694	E. coli LPS	684:694	E. coli LPS	684:694	O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown.
37374879	1	29	theme	natural	198:204	arg1	environment					206:216	the natural environment	194:216	the natural environment	194:216	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	0	30	theme	E.	34:35	arg1	Uptake					42:47	E. coli Uptake	34:47	E. coli Uptake	34:47	O-Polysaccharides of LPS Modulate E. coli Uptake by Acanthamoeba castellanii.
37374879	4	31	theme	wall	538:541	arg1	Lipopolysaccharide					458:475	Lipopolysaccharide	458:475	Lipopolysaccharide (LPS)	458:481	Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall.
37374879	4	31	theme	wall	538:541	arg1	component					496:504	the major component	486:504	the major component of Gram-negative bacterial cell wall	486:541	Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall.
37374879	4	32	theme	cell	533:536	arg1	wall					538:541	Gram-negative bacterial cell wall	509:541	Gram-negative bacterial cell wall	509:541	Lipopolysaccharide (LPS) is the major component of Gram-negative bacterial cell wall.
37374879	9	33	theme	A.	1219:1220	arg1	castellanii					1222:1232	A. castellanii	1219:1232	A. castellanii predation	1219:1242	However, the composition and structure of O-polysaccharide play important roles in providing resistance to A. castellanii predation.
37374879	9	34	theme	important	1176:1184	arg1	roles					1186:1190	important roles	1176:1190	important roles	1176:1190	However, the composition and structure of O-polysaccharide play important roles in providing resistance to A. castellanii predation.
37374879	0	35	dep	E.	34:35	arg1	coli					37:40	coli	37:40	coli	37:40	O-Polysaccharides of LPS Modulate E. coli Uptake by Acanthamoeba castellanii.
37374879	9	36	theme	O-polysaccharide	1154:1169	arg1	structure					1141:1149	structure	1141:1149	structure	1141:1149	However, the composition and structure of O-polysaccharide play important roles in providing resistance to A. castellanii predation.
37374879	9	36	theme	O-polysaccharide	1154:1169	arg1	composition					1125:1135	composition	1125:1135	composition	1125:1135	However, the composition and structure of O-polysaccharide play important roles in providing resistance to A. castellanii predation.
37374879	7	37	theme	E.	931:932	arg1	recognition					939:949	recognition	939:949	recognition	939:949	Here, we investigate how length, structure and composition of LPS affect E. coli recognition and internalization by A. castellanii.
37374879	2	38	theme	many	263:266	arg1	strategies					276:285	many defense strategies	263:285	many defense strategies	263:285	To enhance their survival, bacteria evolved many defense strategies to avoid grazing by protists.
37374879	1	39	theme	bacterial	116:124	arg1	mortality					126:134	bacterial mortality	116:134	bacterial mortality	116:134	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	6	40	theme	Acanthamoeba	737:748	arg1	castellanii					750:760	Acanthamoeba castellanii	737:760	Acanthamoeba castellanii	737:760	O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown.
37374879	1	41	theme	Protozoan	78:86	arg1	cause					107:111	a major cause	99:111	a major cause of bacterial mortality	99:134	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	1	41	theme	Protozoan	78:86	arg1	grazing					88:94	Protozoan grazing	78:94	Protozoan grazing	78:94	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	1	42	from	composition	179:189	arg1	environment					206:216	the natural environment	194:216	the natural environment	194:216	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	1	43	theme	mortality	126:134	arg1	cause					107:111	a major cause	99:111	a major cause of bacterial mortality	99:134	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	1	43	theme	mortality	126:134	arg1	grazing					88:94	Protozoan grazing	78:94	Protozoan grazing	78:94	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37374879	7	44	theme	LPS	920:922	arg1	composition					905:915	composition	905:915	composition	905:915	Here, we investigate how length, structure and composition of LPS affect E. coli recognition and internalization by A. castellanii.
37374879	7	44	theme	LPS	920:922	arg1	structure					891:899	structure	891:899	structure	891:899	Here, we investigate how length, structure and composition of LPS affect E. coli recognition and internalization by A. castellanii.
37374879	7	44	theme	LPS	920:922	arg1	length					883:888	length	883:888	length	883:888	Here, we investigate how length, structure and composition of LPS affect E. coli recognition and internalization by A. castellanii.
37374879	6	45	theme	outermost	664:672	arg1	region					674:679	the outermost region	660:679	the outermost region of E. coli LPS	660:694	O-polysaccharide as the outermost region of E. coli LPS provides protection against predation by Acanthamoeba castellanii; however, the characteristics of O-polysaccharide contribute to this protection remain unknown.
37374879	0	46	theme	Acanthamoeba	52:63	arg1	castellanii					65:75	Acanthamoeba castellanii	52:75	Acanthamoeba castellanii	52:75	O-Polysaccharides of LPS Modulate E. coli Uptake by Acanthamoeba castellanii.
37374879	1	47	from	size	170:173	arg1	environment					206:216	the natural environment	194:216	the natural environment	194:216	Protozoan grazing is a major cause of bacterial mortality and controls bacterial population size and composition in the natural environment.
37377484	14	0	theme	microbiome	993:1002	arg1	diversity					1004:1012	the gut microbiome diversity	985:1012	the gut microbiome diversity	985:1012	Finally, we explored changes in the gut microbiome diversity for mechanistic insights from the gut-liver region.
37377484	5	1	theme	Auricularia	430:440	arg1	var					449:451	that Auricularia cornea var	425:451	that Auricularia cornea var	425:451	Our previous study found that Auricularia cornea var.
37377484	15	2	theme	assessment-insulin	1151:1168	arg1	resistance					1170:1179	homeostasis model assessment-insulin resistance	1133:1179	homeostasis model assessment-insulin resistance (HOMA-IR)	1133:1189	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	15	2	theme	assessment-insulin	1151:1168	arg1	HOMA-IR					1182:1188	HOMA-IR	1182:1188	HOMA-IR	1182:1188	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	2	3	theme	liver	163:167	arg1	disease					169:175	non-alcoholic fatty liver disease	143:175	non-alcoholic fatty liver disease	143:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	3	4	theme	health	245:250	arg1	disease					209:215	Non-alcoholic fatty acid liver disease	178:215	Non-alcoholic fatty acid liver disease (NAFLD)	178:223	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	4	theme	health	245:250	arg1	concern					252:258	a reputed global health concern	228:258	a reputed global health concern	228:258	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	2	5	with	rats	133:136	arg1	disease					169:175	non-alcoholic fatty liver disease	143:175	non-alcoholic fatty liver disease	143:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	2	6	theme	non-alcoholic	143:155	arg1	disease					169:175	non-alcoholic fatty liver disease	143:175	non-alcoholic fatty liver disease	143:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	21	7	dep	findings	2275:2282	arg1	ACP					2296:2298	ACP	2296:2298	ACP	2296:2298	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	21	7	dep	findings	2275:2282	arg1	strategy					2319:2326	a promising strategy	2307:2326	a promising strategy in NAFLD therapy	2307:2343	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	2	8	from	microecology	117:128	arg1	rats					133:136	rats	133:136	rats with non-alcoholic fatty liver disease	133:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	16	9	theme	triglyceride	1305:1316	arg1	levels					1396:1401	triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels	1305:1401	triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD	1305:1439	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	21	10	theme	promising	2309:2317	arg1	ACP					2296:2298	ACP	2296:2298	ACP	2296:2298	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	21	10	theme	promising	2309:2317	arg1	strategy					2319:2326	a promising strategy	2307:2326	a promising strategy in NAFLD therapy	2307:2343	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	14	11	theme	gut	989:991	arg1	diversity					1004:1012	the gut microbiome diversity	985:1012	the gut microbiome diversity	985:1012	Finally, we explored changes in the gut microbiome diversity for mechanistic insights from the gut-liver region.
37377484	15	12	with	supplementation	1086:1100	arg1	ACP					1107:1109	ACP	1107:1109	ACP	1107:1109	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	20	13	theme	intestinal	2108:2117	arg1	microbiota					2119:2128	the intestinal microbiota	2104:2128	the intestinal microbiota	2104:2128	Finally, ACP supplementation regulated the intestinal microbiota to near normal patterns.
37377484	15	14	theme	index	1208:1212	arg1	rates					1214:1218	liver index rates	1202:1218	liver index rates	1202:1218	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	15	15	theme	weight	1224:1229	arg1	gain					1231:1234	weight gain	1224:1234	weight gain (p < 0.05)	1224:1245	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	15	15	theme	weight	1224:1229	arg1	p < 0.05					1237:1244	p < 0.05	1237:1244	p < 0.05	1237:1244	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	20	16	theme	ACP	2074:2076	arg1	supplementation					2078:2092	ACP supplementation	2074:2092	ACP supplementation	2074:2092	Finally, ACP supplementation regulated the intestinal microbiota to near normal patterns.
37377484	17	17	theme	H&E	1574:1576	arg1	technique					1578:1586	H&E technique	1574:1586	H&E technique	1574:1586	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	15	18	theme	body	1192:1195	arg1	fat					1197:1199	body fat	1192:1199	body fat	1192:1199	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	16	19	theme	HDL-C	1275:1279	arg1	levels					1281:1286	HDL-C levels	1275:1286	HDL-C levels	1275:1286	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	3	20	theme	young	284:288	arg1	adults					290:295	young adults	284:295	young adults	284:295	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	21	21	theme	HFD-induced	2176:2186	arg1	NAFLD					2188:2192	HFD-induced NAFLD	2176:2192	HFD-induced NAFLD	2176:2192	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	17	22	theme	lipid	1624:1628	arg1	accumulation					1630:1641	liver lipid accumulation	1618:1641	liver lipid accumulation	1618:1641	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	17	23	theme	liver	1658:1662	arg1	p < 0.05					1679:1686	p < 0.05	1679:1686	p < 0.05	1679:1686	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	17	23	theme	liver	1658:1662	arg1	risks					1672:1676	liver damages risks	1658:1676	liver damages risks (p < 0.05)	1658:1687	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	11	24	theme	high-fat	695:702	arg1	HFD					710:712	HFD	710:712	HFD	710:712	polysaccharides on high-fat diet (HFD)-induced NAFLD and mechanistic actions.
37377484	11	24	theme	high-fat	695:702	arg1	diet					704:707	high-fat diet	695:707	high-fat diet (HFD)	695:713	polysaccharides on high-fat diet (HFD)-induced NAFLD and mechanistic actions.
37377484	16	25	theme	low-density	1352:1362	arg1	cholesterol					1376:1386	low-density lipoprotein cholesterol	1352:1386	low-density lipoprotein cholesterol (LDL-C)	1352:1394	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	16	25	theme	low-density	1352:1362	arg1	LDL-C					1389:1393	LDL-C	1389:1393	LDL-C	1389:1393	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	20	26	theme	normal	2138:2143	arg1	patterns					2145:2152	near normal patterns	2133:2152	near normal patterns	2133:2152	Finally, ACP supplementation regulated the intestinal microbiota to near normal patterns.
37377484	17	27	theme	aspartate	1516:1524	arg1	AST					1544:1546	AST	1544:1546	AST	1544:1546	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	17	27	theme	aspartate	1516:1524	arg1	aminotransferase					1526:1541	aspartate aminotransferase	1516:1541	aspartate aminotransferase (AST)	1516:1547	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	4	28	contain	have	363:366	arg1	polysaccharides					347:361	edible fungi polysaccharides	334:361	edible fungi polysaccharides	334:361	Accumulating evidence suggests that edible fungi polysaccharides have the potential to relieve NAFLD.
37377484	4	28	contain	have	363:366	arg2	potential					372:380	the potential to relieve NAFLD	368:397	the potential to relieve NAFLD	368:397	Accumulating evidence suggests that edible fungi polysaccharides have the potential to relieve NAFLD.
37377484	3	29	theme	fatty	192:196	arg1	NAFLD					218:222	NAFLD	218:222	NAFLD	218:222	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	29	theme	fatty	192:196	arg1	disease					209:215	Non-alcoholic fatty acid liver disease	178:215	Non-alcoholic fatty acid liver disease (NAFLD)	178:223	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	29	theme	fatty	192:196	arg1	concern					252:258	a reputed global health concern	228:258	a reputed global health concern	228:258	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	19	30	theme	ACP	2004:2006	arg1	supplementation					2008:2022	ACP supplementation	2004:2022	ACP supplementation	2004:2022	Proinflammatory markers like IL-6, IL-1β and TNF-α concentrations were decreased by ACP supplementation, accompanied with increased IL-4 levels.
37377484	21	31	theme	liver	2207:2211	arg1	characteristics					2213:2227	liver characteristics	2207:2227	liver characteristics	2207:2227	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	4	32	theme	Accumulating	298:309	arg1	evidence					311:318	Accumulating evidence	298:318	Accumulating evidence	298:318	Accumulating evidence suggests that edible fungi polysaccharides have the potential to relieve NAFLD.
37377484	13	33	theme	ACP	935:937	arg1	potentials					921:930	antioxidant and anti-inflammatory potentials	887:930	antioxidant and anti-inflammatory potentials of ACP	887:937	Then, antioxidant and anti-inflammatory potentials of ACP were studied.
37377484	18	34	theme	antioxidant	1694:1704	arg1	potentials					1706:1715	The antioxidant potentials	1690:1715	The antioxidant potentials of ACP	1690:1722	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	18	35	theme	glutathione	1887:1897	arg1	GSH-PX					1911:1916	GSH-PX	1911:1916	GSH-PX	1911:1916	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	18	35	theme	glutathione	1887:1897	arg1	peroxidase					1899:1908	glutathione peroxidase	1887:1908	glutathione peroxidase (GSH-PX)	1887:1917	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	15	36	theme	homeostasis	1133:1143	arg1	resistance					1170:1179	homeostasis model assessment-insulin resistance	1133:1179	homeostasis model assessment-insulin resistance (HOMA-IR)	1133:1189	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	15	36	theme	homeostasis	1133:1143	arg1	HOMA-IR					1182:1188	HOMA-IR	1182:1188	HOMA-IR	1182:1188	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	17	37	theme	liver	1618:1622	arg1	accumulation					1630:1641	liver lipid accumulation	1618:1641	liver lipid accumulation	1618:1641	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	3	38	theme	liver	203:207	arg1	NAFLD					218:222	NAFLD	218:222	NAFLD	218:222	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	38	theme	liver	203:207	arg1	disease					209:215	Non-alcoholic fatty acid liver disease	178:215	Non-alcoholic fatty acid liver disease (NAFLD)	178:223	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	38	theme	liver	203:207	arg1	concern					252:258	a reputed global health concern	228:258	a reputed global health concern	228:258	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	19	39	dep	IL-6	1949:1952	arg1	concentrations					1971:1984	concentrations	1971:1984	concentrations	1971:1984	Proinflammatory markers like IL-6, IL-1β and TNF-α concentrations were decreased by ACP supplementation, accompanied with increased IL-4 levels.
37377484	0	40	theme	Auricularia	14:24	arg1	var					33:35	Auricularia cornea var	14:35	Auricularia cornea var	14:35	Assessment of Auricularia cornea var.
37377484	12	41	theme	lipid	798:802	arg1	profile					804:810	the histology and hepatic lipid profile	772:810	the histology and hepatic lipid profile of animals	772:821	We first analyzed the histology and hepatic lipid profile of animals to evaluate this variant's ameliorating effects on NAFLD.
37377484	9	42	theme	Auricularia	648:658	arg1	var					667:669	Auricularia cornea var	648:669	Auricularia cornea var	648:669	This study analyzed the protective effects of Auricularia cornea var.
37377484	16	43	theme	cholesterol	1330:1340	arg1	levels					1396:1401	triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels	1305:1401	triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD	1305:1439	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	0	44	theme	var	33:35	arg1	Assessment					0:9	Assessment	0:9	Assessment of Auricularia cornea var.	0:36	Assessment of Auricularia cornea var.
37377484	13	45	theme	anti-inflammatory	903:919	arg1	potentials					921:930	antioxidant and anti-inflammatory potentials	887:930	antioxidant and anti-inflammatory potentials of ACP	887:937	Then, antioxidant and anti-inflammatory potentials of ACP were studied.
37377484	9	46	theme	var	667:669	arg1	effects					637:643	the protective effects	622:643	the protective effects of Auricularia cornea var	622:669	This study analyzed the protective effects of Auricularia cornea var.
37377484	14	47	theme	gut-liver	1048:1056	arg1	region					1058:1063	the gut-liver region	1044:1063	the gut-liver region	1044:1063	Finally, we explored changes in the gut microbiome diversity for mechanistic insights from the gut-liver region.
37377484	19	48	theme	IL-4	2052:2055	arg1	levels					2057:2062	increased IL-4 levels	2042:2062	increased IL-4 levels	2042:2062	Proinflammatory markers like IL-6, IL-1β and TNF-α concentrations were decreased by ACP supplementation, accompanied with increased IL-4 levels.
37377484	19	49	dep	decreased	1991:1999	arg1	accompanied					2025:2035	accompanied	2025:2035	accompanied with increased IL-4 levels	2025:2062	Proinflammatory markers like IL-6, IL-1β and TNF-α concentrations were decreased by ACP supplementation, accompanied with increased IL-4 levels.
37377484	19	50	theme	Proinflammatory	1920:1934	arg1	markers					1936:1942	Proinflammatory markers	1920:1942	Proinflammatory markers like IL-6, IL-1β and TNF-α concentrations	1920:1984	Proinflammatory markers like IL-6, IL-1β and TNF-α concentrations were decreased by ACP supplementation, accompanied with increased IL-4 levels.
37377484	18	51	theme	dismutase	1850:1858	arg1	activities					1825:1834	the activities	1821:1834	the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX)	1821:1917	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	17	52	theme	serum	1475:1479	arg1	ALT					1507:1509	ALT	1507:1509	ALT	1507:1509	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	17	52	theme	serum	1475:1479	arg1	aminotransferase					1489:1504	serum alanine aminotransferase	1475:1504	serum alanine aminotransferase (ALT)	1475:1510	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	12	53	from	effects	863:869	arg1	NAFLD					874:878	NAFLD	874:878	NAFLD	874:878	We first analyzed the histology and hepatic lipid profile of animals to evaluate this variant's ameliorating effects on NAFLD.
37377484	14	54	theme	mechanistic	1018:1028	arg1	insights					1030:1037	mechanistic insights	1018:1037	mechanistic insights from the gut-liver region	1018:1063	Finally, we explored changes in the gut microbiome diversity for mechanistic insights from the gut-liver region.
37377484	19	55	theme	increased	2042:2050	arg1	levels					2057:2062	increased IL-4 levels	2042:2062	increased IL-4 levels	2042:2062	Proinflammatory markers like IL-6, IL-1β and TNF-α concentrations were decreased by ACP supplementation, accompanied with increased IL-4 levels.
37377484	7	56	theme	gut	515:517	arg1	microbiota					519:528	gut microbiota	515:528	gut microbiota	515:528	polysaccharides (ACP) could improve immune by regulating gut microbiota.
37377484	4	57	theme	edible	334:339	arg1	polysaccharides					347:361	edible fungi polysaccharides	334:361	edible fungi polysaccharides	334:361	Accumulating evidence suggests that edible fungi polysaccharides have the potential to relieve NAFLD.
37377484	12	58	theme	ameliorating	850:861	arg1	effects					863:869	this variant's ameliorating effects	835:869	this variant's ameliorating effects on NAFLD	835:878	We first analyzed the histology and hepatic lipid profile of animals to evaluate this variant's ameliorating effects on NAFLD.
37377484	3	59	theme	reputed	230:236	arg1	disease					209:215	Non-alcoholic fatty acid liver disease	178:215	Non-alcoholic fatty acid liver disease (NAFLD)	178:223	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	59	theme	reputed	230:236	arg1	concern					252:258	a reputed global health concern	228:258	a reputed global health concern	228:258	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	2	60	theme	intestinal	106:115	arg1	microecology					117:128	hepatic, antioxidation and intestinal microecology	79:128	hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease	79:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	5	61	theme	cornea	442:447	arg1	var					449:451	that Auricularia cornea var	425:451	that Auricularia cornea var	425:451	Our previous study found that Auricularia cornea var.
37377484	13	62	theme	antioxidant	887:897	arg1	potentials					921:930	antioxidant and anti-inflammatory potentials	887:930	antioxidant and anti-inflammatory potentials of ACP	887:937	Then, antioxidant and anti-inflammatory potentials of ACP were studied.
37377484	2	63	theme	fatty	157:161	arg1	disease					169:175	non-alcoholic fatty liver disease	143:175	non-alcoholic fatty liver disease	143:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	15	64	theme	model	1145:1149	arg1	resistance					1170:1179	homeostasis model assessment-insulin resistance	1133:1179	homeostasis model assessment-insulin resistance (HOMA-IR)	1133:1189	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	15	64	theme	model	1145:1149	arg1	HOMA-IR					1182:1188	HOMA-IR	1182:1188	HOMA-IR	1182:1188	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	18	65	theme	malondialdehyde	1785:1799	arg1	levels					1775:1780	the hepatic levels	1763:1780	the hepatic levels of malondialdehyde (MDA)	1763:1805	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	14	66	from	changes	974:980	arg1	diversity					1004:1012	the gut microbiome diversity	985:1012	the gut microbiome diversity	985:1012	Finally, we explored changes in the gut microbiome diversity for mechanistic insights from the gut-liver region.
37377484	3	67	theme	global	238:243	arg1	disease					209:215	Non-alcoholic fatty acid liver disease	178:215	Non-alcoholic fatty acid liver disease (NAFLD)	178:223	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	67	theme	global	238:243	arg1	concern					252:258	a reputed global health concern	228:258	a reputed global health concern	228:258	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	8	68	theme	NAFLD-alleviating	544:560	arg1	potentials					562:571	its NAFLD-alleviating potentials	540:571	its NAFLD-alleviating potentials	540:571	However, its NAFLD-alleviating potentials have been scarcely reported.
37377484	11	69	from	polysaccharides	676:690	arg1	HFD					710:712	HFD	710:712	HFD	710:712	polysaccharides on high-fat diet (HFD)-induced NAFLD and mechanistic actions.
37377484	11	69	from	polysaccharides	676:690	arg1	diet					704:707	high-fat diet	695:707	high-fat diet (HFD)	695:713	polysaccharides on high-fat diet (HFD)-induced NAFLD and mechanistic actions.
37377484	2	70	theme	polysaccharides	42:56	arg1	potential					58:66	polysaccharides potential	42:66	polysaccharides potential	42:66	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	12	71	theme	animals	815:821	arg1	profile					804:810	the histology and hepatic lipid profile	772:810	the histology and hepatic lipid profile of animals	772:821	We first analyzed the histology and hepatic lipid profile of animals to evaluate this variant's ameliorating effects on NAFLD.
37377484	15	72	theme	liver	1202:1206	arg1	rates					1214:1218	liver index rates	1202:1218	liver index rates	1202:1218	Results showed that supplementation with ACP substantially reduced homeostasis model assessment-insulin resistance (HOMA-IR), body fat, liver index rates and weight gain (p < 0.05).
37377484	16	73	theme	cholesterol	1376:1386	arg1	levels					1396:1401	triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels	1305:1401	triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD	1305:1439	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	2	74	theme	hepatic	79:85	arg1	microecology					117:128	hepatic, antioxidation and intestinal microecology	79:128	hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease	79:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37377484	17	75	theme	aminotransferase	1489:1504	arg1	levels					1549:1554	the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels	1471:1554	the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels	1471:1554	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	20	76	theme	near	2133:2136	arg1	patterns					2145:2152	near normal patterns	2133:2152	near normal patterns	2133:2152	Finally, ACP supplementation regulated the intestinal microbiota to near normal patterns.
37377484	17	77	theme	damages	1664:1670	arg1	p < 0.05					1679:1686	p < 0.05	1679:1686	p < 0.05	1679:1686	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	17	77	theme	damages	1664:1670	arg1	risks					1672:1676	liver damages risks	1658:1676	liver damages risks (p < 0.05)	1658:1687	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	5	78	theme	previous	404:411	arg1	study					413:417	Our previous study	400:417	Our previous study	400:417	Our previous study found that Auricularia cornea var.
37377484	21	79	theme	NAFLD	2331:2335	arg1	therapy					2337:2343	NAFLD therapy	2331:2343	NAFLD therapy	2331:2343	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	3	80	theme	Non-alcoholic	178:190	arg1	NAFLD					218:222	NAFLD	218:222	NAFLD	218:222	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	80	theme	Non-alcoholic	178:190	arg1	disease					209:215	Non-alcoholic fatty acid liver disease	178:215	Non-alcoholic fatty acid liver disease (NAFLD)	178:223	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	80	theme	Non-alcoholic	178:190	arg1	concern					252:258	a reputed global health concern	228:258	a reputed global health concern	228:258	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	11	81	theme	NAFLD	723:727	arg1	actions					745:751	NAFLD and mechanistic actions	723:751	NAFLD and mechanistic actions	723:751	polysaccharides on high-fat diet (HFD)-induced NAFLD and mechanistic actions.
37377484	17	82	theme	aminotransferase	1526:1541	arg1	levels					1549:1554	the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels	1471:1554	the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels	1471:1554	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	16	83	theme	lipoprotein	1364:1374	arg1	cholesterol					1376:1386	low-density lipoprotein cholesterol	1352:1386	low-density lipoprotein cholesterol (LDL-C)	1352:1394	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	16	83	theme	lipoprotein	1364:1374	arg1	LDL-C					1389:1393	LDL-C	1389:1393	LDL-C	1389:1393	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	18	84	theme	hepatic	1767:1773	arg1	levels					1775:1780	the hepatic levels	1763:1780	the hepatic levels of malondialdehyde (MDA)	1763:1805	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	18	85	theme	peroxidase	1899:1908	arg1	activities					1825:1834	the activities	1821:1834	the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX)	1821:1917	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	9	86	theme	protective	626:635	arg1	effects					637:643	the protective effects	622:643	the protective effects of Auricularia cornea var	622:669	This study analyzed the protective effects of Auricularia cornea var.
37377484	3	87	theme	acid	198:201	arg1	NAFLD					218:222	NAFLD	218:222	NAFLD	218:222	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	87	theme	acid	198:201	arg1	disease					209:215	Non-alcoholic fatty acid liver disease	178:215	Non-alcoholic fatty acid liver disease (NAFLD)	178:223	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	3	87	theme	acid	198:201	arg1	concern					252:258	a reputed global health concern	228:258	a reputed global health concern	228:258	Non-alcoholic fatty acid liver disease (NAFLD) is a reputed global health concern, affecting children and young adults.
37377484	0	88	theme	cornea	26:31	arg1	var					33:35	Auricularia cornea var	14:35	Auricularia cornea var	14:35	Assessment of Auricularia cornea var.
37377484	12	89	theme	histology	776:784	arg1	profile					804:810	the histology and hepatic lipid profile	772:810	the histology and hepatic lipid profile of animals	772:821	We first analyzed the histology and hepatic lipid profile of animals to evaluate this variant's ameliorating effects on NAFLD.
37377484	11	90	theme	mechanistic	733:743	arg1	actions					745:751	NAFLD and mechanistic actions	723:751	NAFLD and mechanistic actions	723:751	polysaccharides on high-fat diet (HFD)-induced NAFLD and mechanistic actions.
37377484	14	91	from	region	1058:1063	arg1	insights					1030:1037	mechanistic insights	1018:1037	mechanistic insights from the gut-liver region	1018:1063	Finally, we explored changes in the gut microbiome diversity for mechanistic insights from the gut-liver region.
37377484	12	92	theme	hepatic	790:796	arg1	profile					804:810	the histology and hepatic lipid profile	772:810	the histology and hepatic lipid profile of animals	772:821	We first analyzed the histology and hepatic lipid profile of animals to evaluate this variant's ameliorating effects on NAFLD.
37377484	17	93	theme	ACP	1442:1444	arg1	mediation					1446:1454	ACP mediation	1442:1454	ACP mediation	1442:1454	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	9	94	theme	cornea	660:665	arg1	var					667:669	Auricularia cornea var	648:669	Auricularia cornea var	648:669	This study analyzed the protective effects of Auricularia cornea var.
37377484	16	95	theme	total	1324:1328	arg1	cholesterol					1330:1340	total cholesterol	1324:1340	total cholesterol (TC)	1324:1345	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	16	95	theme	total	1324:1328	arg1	TC					1343:1344	TC	1343:1344	TC	1343:1344	This variant also improved HDL-C levels while decreasing triglyceride (TG), total cholesterol (TC), and low-density lipoprotein cholesterol (LDL-C) levels which were initially triggered by HFD.
37377484	21	96	theme	colonic	2244:2250	arg1	composition					2258:2268	colonic flora composition	2244:2268	colonic flora composition	2244:2268	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	21	96	theme	colonic	2244:2250	arg1	findings					2275:2282	our findings	2271:2282	our findings assert that ACP can be a promising strategy in NAFLD therapy	2271:2343	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	18	97	theme	ACP	1720:1722	arg1	potentials					1706:1715	The antioxidant potentials	1690:1715	The antioxidant potentials of ACP	1690:1722	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	4	98	theme	fungi	341:345	arg1	polysaccharides					347:361	edible fungi polysaccharides	334:361	edible fungi polysaccharides	334:361	Accumulating evidence suggests that edible fungi polysaccharides have the potential to relieve NAFLD.
37377484	18	99	theme	superoxide	1839:1848	arg1	dismutase					1850:1858	superoxide dismutase	1839:1858	superoxide dismutase (SOD)	1839:1864	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	18	99	theme	superoxide	1839:1848	arg1	SOD					1861:1863	SOD	1861:1863	SOD	1861:1863	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	17	100	theme	alanine	1481:1487	arg1	ALT					1507:1509	ALT	1507:1509	ALT	1507:1509	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	17	100	theme	alanine	1481:1487	arg1	aminotransferase					1489:1504	serum alanine aminotransferase	1475:1504	serum alanine aminotransferase (ALT)	1475:1510	ACP mediation also decreased the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels considerably with H&E technique indicating that it can reduce liver lipid accumulation, thus lowering liver damages risks (p < 0.05).
37377484	21	101	from	strategy	2319:2326	arg1	therapy					2337:2343	NAFLD therapy	2331:2343	NAFLD therapy	2331:2343	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	21	102	theme	flora	2252:2256	arg1	composition					2258:2268	colonic flora composition	2244:2268	colonic flora composition	2244:2268	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	21	102	theme	flora	2252:2256	arg1	findings					2275:2282	our findings	2271:2282	our findings assert that ACP can be a promising strategy in NAFLD therapy	2271:2343	In all, ACP protects HFD-induced NAFLD by improving liver characteristics and regulating colonic flora composition, our findings assert that ACP can be a promising strategy in NAFLD therapy.
37377484	18	103	theme	catalase	1867:1874	arg1	activities					1825:1834	the activities	1821:1834	the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX)	1821:1917	The antioxidant potentials of ACP were also demonstrated as it decreased the hepatic levels of malondialdehyde (MDA) and increased the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-PX).
37377484	2	104	theme	antioxidation	88:100	arg1	microecology					117:128	hepatic, antioxidation and intestinal microecology	79:128	hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease	79:175	polysaccharides potential to improve hepatic, antioxidation and intestinal microecology in rats with non-alcoholic fatty liver disease.
37278859	3	0	dep	14.4 kDa	581:588	arg1	to					578:579	to	578:579	to	578:579	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	0	1	theme	polysaccharides	94:108	arg1	activities					58:67	inhibitory activities	47:67	inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights	47:141	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	3	2	with	glucans	519:525	arg1	weights					552:558	different molecular weights	532:558	different molecular weights ranging from 3.12 to 14.4 kDa	532:588	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	3	3	theme	glucans	519:525	arg1	glucans					519:525	glucans	519:525	glucans with different molecular weights ranging from 3.12 to 14.4 kDa	519:588	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	3	3	theme	glucans	519:525	arg1	polysaccharides					487:501	Four polysaccharides	482:501	Four polysaccharides	482:501	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	3	3	theme	glucans	519:525	arg1	group					510:514	a group	508:514	a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa	508:588	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	0	4	theme	molecular	125:133	arg1	weights					135:141	different molecular weights	115:141	different molecular weights	115:141	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	0	5	with	polysaccharides	94:108	arg1	weights					135:141	different molecular weights	115:141	different molecular weights	115:141	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	4	6	theme	concentration-dependent	755:777	arg1	activity					790:797	concentration-dependent inhibitory activity	755:797	concentration-dependent inhibitory activity	755:797	Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase.
37278859	0	7	theme	different	115:123	arg1	weights					135:141	different molecular weights	115:141	different molecular weights	115:141	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	5	8	with	MCPb	817:820	arg1	weights					887:893	moderate molecular weights	868:893	moderate molecular weights	868:893	MCPb (Mw = 10.1 kDa) and MCPc (Mw = 5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.
37278859	5	9	theme	inhibitory	912:921	arg1	activity					923:930	higher inhibitory activity	905:930	higher inhibitory activity	905:930	MCPb (Mw = 10.1 kDa) and MCPc (Mw = 5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.
37278859	3	10	with	linkages	655:662	arg1	branches					669:676	branches	669:676	branches attached to C-3 and C-6	669:700	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	2	11	dep	composition	430:440	arg1	determination					442:454	determination	442:454	determination	442:454	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	12	theme	instrumental	284:295	arg1	sugar					321:325	total sugar	315:325	total sugar	315:325	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	12	theme	instrumental	284:295	arg1	composition					430:440	monosaccharide composition determination and methylation analyses	415:479	composition	430:440	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	12	theme	instrumental	284:295	arg1	UV					375:376	UV	375:376	UV	375:376	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	12	theme	instrumental	284:295	arg1	methods					297:303	chemical and instrumental methods	271:303	chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses	271:479	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	12	theme	instrumental	284:295	arg1	acid					335:338	uronic acid	328:338	uronic acid	328:338	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	12	theme	instrumental	284:295	arg1	IR					379:380	IR	379:380	IR	379:380	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	1	13	dep	polysaccharides	149:163	arg1	MCPb					172:175	MCPb	172:175	MCPb	172:175	Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp.
37278859	1	13	dep	polysaccharides	149:163	arg1	MCPa					166:169	MCPa	166:169	MCPa	166:169	Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp.
37278859	1	13	dep	polysaccharides	149:163	arg1	MCPd					184:187	MCPd	184:187	MCPd	184:187	Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp.
37278859	1	13	dep	polysaccharides	149:163	arg1	MCPc					178:181	MCPc	178:181	MCPc	178:181	Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp.
37278859	2	14	theme	NMR	386:388	arg1	spectroscopy					390:401	NMR spectroscopy	386:401	NMR spectroscopy	386:401	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	3	15	attach	attached	678:685	arg2	branches					669:676	branches	669:676	branches attached to C-3 and C-6	669:700	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	3	15	attach	attached	678:685	arg1	C-6					698:700	C-6	698:700	C-6	698:700	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	3	15	attach	attached	678:685	arg1	C-3					690:692	C-3	690:692	C-3	690:692	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	2	16	theme	uronic	328:333	arg1	acid					335:338	uronic acid	328:338	uronic acid	328:338	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	5	17	theme	molecular	877:885	arg1	weights					887:893	moderate molecular weights	868:893	moderate molecular weights	868:893	MCPb (Mw = 10.1 kDa) and MCPc (Mw = 5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.
37278859	0	18	theme	Structural	0:9	arg1	characterizations					11:27	Structural characterizations	0:27	Structural characterizations	0:27	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	2	19	dep	acid	335:338	arg1	determination					360:372	determination	360:372	determination	360:372	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	20	theme	monosaccharide	415:428	arg1	composition					430:440	monosaccharide composition determination and methylation analyses	415:479	composition	430:440	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	3	21	theme	molecular	542:550	arg1	weights					552:558	different molecular weights	532:558	different molecular weights ranging from 3.12 to 14.4 kDa	532:588	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	2	22	theme	total	315:319	arg1	sugar					321:325	total sugar	315:325	total sugar	315:325	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	5	23	with	MCPc	842:845	arg1	weights					887:893	moderate molecular weights	868:893	moderate molecular weights	868:893	MCPb (Mw = 10.1 kDa) and MCPc (Mw = 5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.
37278859	3	24	theme	-glucose	646:653	arg1	linkages					655:662	(1→4)-glucose linkages	641:662	(1→4)-glucose linkages with branches attached to C-3 and C-6	641:700	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	1	25	theme	Lepidium	209:216	arg1	Walp					226:229	Lepidium meyenii Walp	209:229	Lepidium meyenii Walp	209:229	Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp.
37278859	0	26	theme	inhibitory	47:56	arg1	activities					58:67	inhibitory activities	47:67	inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights	47:141	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	3	27	theme	similar	604:610	arg1	chain					621:625	a similar backbone chain	602:625	a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6	602:700	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	1	28	theme	meyenii	218:224	arg1	Walp					226:229	Lepidium meyenii Walp	209:229	Lepidium meyenii Walp	209:229	Four polysaccharides (MCPa, MCPb, MCPc, MCPd) were obtained from Lepidium meyenii Walp.
37278859	3	29	theme	1→4	642:644	arg1	linkages					655:662	(1→4)-glucose linkages	641:662	(1→4)-glucose linkages with branches attached to C-3 and C-6	641:700	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	2	30	theme	methylation	460:470	arg1	analyses					472:479	methylation analyses	460:479	methylation analyses	460:479	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	4	31	contain	had	751:753	arg2	activity					790:797	concentration-dependent inhibitory activity	755:797	concentration-dependent inhibitory activity	755:797	Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase.
37278859	4	31	contain	had	751:753	arg1	MCPs					746:749	MCPs	746:749	MCPs	746:749	Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase.
37278859	2	32	theme	protein	344:350	arg1	content					352:358	protein content	344:358	protein content	344:358	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	33	theme	chemical	271:278	arg1	sugar					321:325	total sugar	315:325	total sugar	315:325	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	33	theme	chemical	271:278	arg1	composition					430:440	monosaccharide composition determination and methylation analyses	415:479	composition	430:440	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	33	theme	chemical	271:278	arg1	UV					375:376	UV	375:376	UV	375:376	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	33	theme	chemical	271:278	arg1	methods					297:303	chemical and instrumental methods	271:303	chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses	271:479	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	33	theme	chemical	271:278	arg1	acid					335:338	uronic acid	328:338	uronic acid	328:338	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	2	33	theme	chemical	271:278	arg1	IR					379:380	IR	379:380	IR	379:380	Their structures were characterized by chemical and instrumental methods including total sugar, uronic acid and protein content determination, UV, IR and NMR spectroscopy, as well as monosaccharide composition determination and methylation analyses.
37278859	4	34	theme	inhibitory	779:788	arg1	activity					790:797	concentration-dependent inhibitory activity	755:797	concentration-dependent inhibitory activity	755:797	Furthermore, bioactivity assay showed that MCPs had concentration-dependent inhibitory activity on α-glucosidase.
37278859	5	35	theme	higher	905:910	arg1	activity					923:930	higher inhibitory activity	905:930	higher inhibitory activity	905:930	MCPb (Mw = 10.1 kDa) and MCPc (Mw = 5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.
37278859	0	36	theme	meyenii	86:92	arg1	polysaccharides					94:108	four Lepidium meyenii polysaccharides	72:108	four Lepidium meyenii polysaccharides with different molecular weights	72:141	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	3	37	theme	backbone	612:619	arg1	chain					621:625	a similar backbone chain	602:625	a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6	602:700	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
37278859	5	38	theme	moderate	868:875	arg1	weights					887:893	moderate molecular weights	868:893	moderate molecular weights	868:893	MCPb (Mw = 10.1 kDa) and MCPc (Mw = 5.62 kDa) with moderate molecular weights exhibited higher inhibitory activity compared with MCPa and MCPd.
37278859	0	39	theme	Lepidium	77:84	arg1	polysaccharides					94:108	four Lepidium meyenii polysaccharides	72:108	four Lepidium meyenii polysaccharides with different molecular weights	72:141	Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights.
37278859	3	40	theme	different	532:540	arg1	weights					552:558	different molecular weights	532:558	different molecular weights ranging from 3.12 to 14.4 kDa	532:588	Four polysaccharides were a group of glucans with different molecular weights ranging from 3.12 to 14.4 kDa, and shared a similar backbone chain consisting of (1→4)-glucose linkages with branches attached to C-3 and C-6.
35777121	4	0	theme	favorable	845:853	arg1	basis					855:859	the favorable basis	841:859	the favorable basis for the pharmacodynamic material basis of LRH	841:905	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	11	1	with	137	2090:2092	arg1	UC					2123:2124	UC	2123:2124	UC	2123:2124	137 of their common targets with UC were further predicted.
35777121	17	2	theme	PI3K/AKT	3157:3164	arg1	pathway					3166:3172	the PI3K/AKT pathway	3153:3172	the PI3K/AKT pathway	3153:3172	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	13	3	theme	intestinal	2539:2548	arg1	barrier					2566:2572	the intestinal epithelial cell barrier	2535:2572	the intestinal epithelial cell barrier	2535:2572	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	17	4	theme	DSS-induced	2958:2968	arg1	UC					2970:2971	DSS-induced UC	2958:2971	DSS-induced UC	2958:2971	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	7	5	theme	activity	1422:1429	arg1	DAI					1438:1440	DAI	1438:1440	DAI	1438:1440	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	5	theme	activity	1422:1429	arg1	index					1431:1435	disease activity index	1414:1435	disease activity index (DAI)	1414:1441	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	12	6	theme	enrichment	2170:2179	arg1	analysis					2181:2188	KEGG pathway enrichment analysis	2157:2188	KEGG pathway enrichment analysis	2157:2188	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	13	7	theme	cell	2561:2564	arg1	barrier					2566:2572	the intestinal epithelial cell barrier	2535:2572	the intestinal epithelial cell barrier	2535:2572	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	2	8	theme	LRH	283:285	arg1	flavonoids					287:296	And LRH flavonoids	279:296	flavonoids	287:296	And LRH flavonoids have excellent anti-inflammatory and antioxidant pharmacological activities.
35777121	11	9	theme	targets	2110:2116	arg1	137					2090:2092	137	2090:2092	137 of their common targets with UC	2090:2124	137 of their common targets with UC were further predicted.
35777121	4	10	theme	herbal	644:649	arg1	resources					651:659	Tibetan herbal resources	636:659	Tibetan herbal resources	636:659	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	17	11	theme	gut	3098:3100	arg1	microenvironment					3102:3117	the gut microenvironment	3094:3117	the gut microenvironment	3094:3117	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	8	12	theme	region	1689:1694	arg1	sequencing					1665:1674	high-throughput sequencing	1649:1674	high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms	1649:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	1	13	theme	rupicola	197:204	arg1	LRH					223:225	LRH	223:225	LRH	223:225	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	13	theme	rupicola	197:204	arg1	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	13	theme	rupicola	197:204	arg1	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	7	14	theme	colon	1400:1404	arg1	length					1406:1411	colon length	1400:1411	colon length	1400:1411	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	0	15	theme	PI3K/AKT	159:166	arg1	pathway					168:174	PI3K/AKT pathway	159:174	PI3K/AKT pathway	159:174	Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway.
35777121	15	16	theme	gut	2792:2794	arg1	microbiome					2796:2805	gut microbiome	2792:2805	gut microbiome	2792:2805	16S rDNA sequence analysis indicated that LRH flavonoids promoted the recovery of gut microbiome.
35777121	2	17	theme	anti-inflammatory	313:329	arg1	activities					363:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	And LRH flavonoids have excellent anti-inflammatory and antioxidant pharmacological activities.
35777121	8	18	theme	16S	1699:1701	arg1	rDNA					1718:1721	rDNA	1718:1721	rDNA	1718:1721	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	8	18	theme	16S	1699:1701	arg1	DNA					1713:1715	16S ribosomal DNA	1699:1715	16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms	1699:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	14	19	theme	dynamic	2658:2664	arg1	balance					2666:2672	dynamic balance	2658:2672	dynamic balance	2658:2672	The mRNA and proteins expression levels of inflammatory mediators were returned to dynamic balance following LRH flavonoids treatment.
35777121	4	20	theme	LRH	903:905	arg1	basis					894:898	the pharmacodynamic material basis	865:898	the pharmacodynamic material basis of LRH	865:905	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	9	21	theme	underlying	1900:1909	arg1	mechanisms					1921:1930	their underlying molecular mechanisms	1894:1930	their underlying molecular mechanisms	1894:1930	Finally, the key regulatory proteins in the PI3K/AKT pathways were measured to investigate their underlying molecular mechanisms.
35777121	5	22	theme	chromatography	1038:1051	arg1	UPLC-MS/MS					1079:1088	UPLC-MS/MS	1079:1088	UPLC-MS/MS	1079:1088	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	22	theme	chromatography	1038:1051	arg1	spectrometry					1065:1076	ultra-performance liquid chromatography tandem mass spectrometry	1013:1076	ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)	1013:1089	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	8	23	theme	DNA	1713:1715	arg1	region					1689:1694	the V3-V4 region	1679:1694	the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms	1679:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	7	24	theme	body	1382:1385	arg1	loss					1394:1397	body weight loss	1382:1397	body weight loss	1382:1397	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	10	25	theme	flavonoid	1959:1967	arg1	components					1969:1978	37 LRH flavonoid components	1952:1978	37 LRH flavonoid components	1952:1978	RESULTS A total of 37 LRH flavonoid components were identified and detected by UPLC-MS/MS, and 12 potential active components were obtained after screening.
35777121	13	26	theme	oxidative	2503:2511	arg1	stress					2513:2518	oxidative stress	2503:2518	oxidative stress	2503:2518	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	5	27	theme	mass	1060:1063	arg1	UPLC-MS/MS					1079:1088	UPLC-MS/MS	1079:1088	UPLC-MS/MS	1079:1088	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	27	theme	mass	1060:1063	arg1	spectrometry					1065:1076	ultra-performance liquid chromatography tandem mass spectrometry	1013:1076	ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)	1013:1089	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	18	28	theme	great	3231:3235	arg1	potential					3237:3245	great potential	3231:3245	great potential for becoming an excellent drug for the treatment of UC	3231:3300	This finding demonstrated that LRH flavonoids possessed great potential for becoming an excellent drug for the treatment of UC.
35777121	14	29	theme	mediators	2631:2639	arg1	levels					2608:2613	The mRNA and proteins expression levels	2575:2613	The mRNA and proteins expression levels of inflammatory mediators	2575:2639	The mRNA and proteins expression levels of inflammatory mediators were returned to dynamic balance following LRH flavonoids treatment.
35777121	4	30	theme	pharmacodynamic	869:883	arg1	basis					894:898	the pharmacodynamic material basis	865:898	the pharmacodynamic material basis of LRH	865:905	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	31	theme	underlying	695:704	arg1	mechanisms					716:725	the underlying molecular mechanisms	691:725	the underlying molecular mechanisms	691:725	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	6	32	theme	pharmacology	1295:1306	arg1	analysis					1308:1315	network pharmacology analysis	1287:1315	network pharmacology analysis	1287:1315	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	12	33	dep	analysis	2181:2188	arg1	GO					2150:2151	GO	2150:2151	GO	2150:2151	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	7	34	theme	LRH	1360:1362	arg1	efficacy					1348:1355	pharmacological efficacy	1332:1355	pharmacological efficacy of LRH	1332:1362	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	6	35	theme	potential	1186:1194	arg1	Meanwhile					1169:1177	Meanwhile	1169:1177	Meanwhile	1169:1177	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	6	35	theme	potential	1186:1194	arg1	targets					1196:1202	their potential targets	1180:1202	their potential targets	1180:1202	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	13	36	theme	colon	2478:2482	arg1	length					2484:2489	colon length	2478:2489	colon length	2478:2489	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	5	37	theme	ultra-performance	1013:1029	arg1	UPLC-MS/MS					1079:1088	UPLC-MS/MS	1079:1088	UPLC-MS/MS	1079:1088	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	37	theme	ultra-performance	1013:1029	arg1	spectrometry					1065:1076	ultra-performance liquid chromatography tandem mass spectrometry	1013:1076	ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)	1013:1089	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	17	38	theme	barrier	3062:3068	arg1	function					3070:3077	epithelial barrier function	3051:3077	epithelial barrier function	3051:3077	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	8	39	theme	community	1738:1746	arg1	composition					1748:1758	bacterial community composition	1728:1758	bacterial community composition	1728:1758	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	14	40	theme	expression	2597:2606	arg1	levels					2608:2613	The mRNA and proteins expression levels	2575:2613	The mRNA and proteins expression levels of inflammatory mediators	2575:2639	The mRNA and proteins expression levels of inflammatory mediators were returned to dynamic balance following LRH flavonoids treatment.
35777121	4	41	theme	therapeutic	666:676	arg1	efficacy					678:685	the therapeutic efficacy	662:685	the therapeutic efficacy	662:685	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	15	42	theme	sequence	2719:2726	arg1	analysis					2728:2735	16S rDNA sequence analysis	2710:2735	16S rDNA sequence analysis	2710:2735	16S rDNA sequence analysis indicated that LRH flavonoids promoted the recovery of gut microbiome.
35777121	7	43	theme	junction	1550:1557	arg1	proteins					1559:1566	tight junction proteins	1544:1566	tight junction proteins	1544:1566	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	4	44	theme	new	961:963	arg1	drugs					965:969	new drugs	961:969	new drugs for the treatment of UC	961:993	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	0	45	theme	intestinal	94:103	arg1	barrier					105:111	intestinal barrier	94:111	intestinal barrier	94:111	Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway.
35777121	1	46	theme	BACKGROUND	177:186	arg1	LRH					223:225	LRH	223:225	LRH	223:225	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	46	theme	BACKGROUND	177:186	arg1	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	46	theme	BACKGROUND	177:186	arg1	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	4	47	theme	scientific	923:932	arg1	basis					934:938	a scientific basis	921:938	a scientific basis for the discovery of new drugs for the treatment of UC	921:993	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	12	48	theme	docking	2204:2210	arg1	experiments					2212:2222	molecular docking experiments	2194:2222	molecular docking experiments	2194:2222	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	8	49	theme	MiSeq	1786:1790	arg1	platforms					1792:1800	Illumina MiSeq platforms	1777:1800	Illumina MiSeq platforms	1777:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	13	50	theme	weight	2447:2452	arg1	loss					2454:2457	the body weight loss	2438:2457	the body weight loss	2438:2457	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	17	51	theme	colon	3000:3004	arg1	inflammation					3006:3017	colon inflammation	3000:3017	colon inflammation	3000:3017	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	0	52	theme	gut	118:120	arg1	microbiome					122:131	gut microbiome	118:131	gut microbiome	118:131	Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway.
35777121	17	53	theme	multifaceted	2918:2929	arg1	effects					2942:2948	multifaceted protective effects	2918:2948	multifaceted protective effects against DSS-induced UC in mice	2918:2979	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	7	54	theme	pharmacological	1332:1346	arg1	efficacy					1348:1355	pharmacological efficacy	1332:1355	pharmacological efficacy of LRH	1332:1362	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	4	55	theme	UC	992:993	arg1	treatment					979:987	the treatment	975:987	the treatment of UC	975:993	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	3	56	theme	LRH	520:522	arg1	waste					511:515	the waste	507:515	the waste of LRH as a medicinal resource	507:546	However, the specific effects of LRH and its mechanism remain unknown, and there is a deficiency of systematic research, leading to the waste of LRH as a medicinal resource.
35777121	4	57	theme	LRH	730:732	arg1	flavonoids					734:743	LRH flavonoids	730:743	LRH flavonoids	730:743	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	5	58	theme	components	1150:1159	arg1	detection					1123:1131	detection	1123:1131	detection	1123:1131	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	58	theme	components	1150:1159	arg1	identification					1104:1117	identification	1104:1117	identification	1104:1117	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	59	theme	liquid	1031:1036	arg1	UPLC-MS/MS					1079:1088	UPLC-MS/MS	1079:1088	UPLC-MS/MS	1079:1088	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	59	theme	liquid	1031:1036	arg1	spectrometry					1065:1076	ultra-performance liquid chromatography tandem mass spectrometry	1013:1076	ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)	1013:1089	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	60	theme	LRH	1164:1166	arg1	components					1150:1159	the flavonoid components	1136:1159	the flavonoid components of LRH	1136:1166	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	7	61	theme	messenger	1519:1527	arg1	mRNA					1534:1537	mRNA	1534:1537	mRNA	1534:1537	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	61	theme	messenger	1519:1527	arg1	RNA					1529:1531	messenger RNA	1519:1531	messenger RNA (mRNA)	1519:1538	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	15	62	theme	LRH	2752:2754	arg1	flavonoids					2756:2765	LRH flavonoids	2752:2765	LRH flavonoids	2752:2765	16S rDNA sequence analysis indicated that LRH flavonoids promoted the recovery of gut microbiome.
35777121	2	63	contain	have	298:301	arg1	flavonoids					287:296	And LRH flavonoids	279:296	flavonoids	287:296	And LRH flavonoids have excellent anti-inflammatory and antioxidant pharmacological activities.
35777121	2	63	contain	have	298:301	arg2	activities					363:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	And LRH flavonoids have excellent anti-inflammatory and antioxidant pharmacological activities.
35777121	7	64	theme	mediators	1508:1516	arg1	length					1406:1411	colon length	1400:1411	colon length	1400:1411	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	64	theme	mediators	1508:1516	arg1	DAI					1438:1440	DAI	1438:1440	DAI	1438:1440	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	64	theme	mediators	1508:1516	arg1	levels					1485:1490	the expression levels	1470:1490	the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins	1470:1566	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	64	theme	mediators	1508:1516	arg1	index					1431:1435	disease activity index	1414:1435	disease activity index (DAI)	1414:1441	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	64	theme	mediators	1508:1516	arg1	observation					1454:1464	histology observation	1444:1464	histology observation	1444:1464	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	64	theme	mediators	1508:1516	arg1	loss					1394:1397	body weight loss	1382:1397	body weight loss	1382:1397	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	14	65	theme	proteins	2588:2595	arg1	levels					2608:2613	The mRNA and proteins expression levels	2575:2613	The mRNA and proteins expression levels of inflammatory mediators	2575:2639	The mRNA and proteins expression levels of inflammatory mediators were returned to dynamic balance following LRH flavonoids treatment.
35777121	12	66	theme	KEGG	2157:2160	arg1	analysis					2181:2188	KEGG pathway enrichment analysis	2157:2188	KEGG pathway enrichment analysis	2157:2188	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	17	67	theme	LRH	2893:2895	arg1	flavonoids					2897:2906	CONCLUSIONS LRH flavonoids	2881:2906	CONCLUSIONS LRH flavonoids	2881:2906	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	0	68	theme	dysregulated	57:68	arg1	responses					83:91	dysregulated inflammatory responses	57:91	dysregulated inflammatory responses	57:91	Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway.
35777121	10	69	theme	active	2041:2046	arg1	components					2048:2057	12 potential active components	2028:2057	12 potential active components	2028:2057	RESULTS A total of 37 LRH flavonoid components were identified and detected by UPLC-MS/MS, and 12 potential active components were obtained after screening.
35777121	2	70	theme	pharmacological	347:361	arg1	activities					363:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	And LRH flavonoids have excellent anti-inflammatory and antioxidant pharmacological activities.
35777121	7	71	theme	expression	1474:1483	arg1	levels					1485:1490	the expression levels	1470:1490	the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins	1470:1566	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	13	72	theme	LRH	2362:2364	arg1	flavonoids					2366:2375	LRH flavonoids	2362:2375	LRH flavonoids	2362:2375	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	9	73	theme	regulatory	1820:1829	arg1	proteins					1831:1838	the key regulatory proteins	1812:1838	the key regulatory proteins in the PI3K/AKT pathways	1812:1863	Finally, the key regulatory proteins in the PI3K/AKT pathways were measured to investigate their underlying molecular mechanisms.
35777121	4	74	theme	ulcerative	785:794	arg1	UC					805:806	UC	805:806	UC	805:806	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	74	theme	ulcerative	785:794	arg1	colitis					796:802	dextran sulfate sodium (DSS)-induced ulcerative colitis	748:802	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC)	748:807	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	17	75	theme	protective	2931:2940	arg1	effects					2942:2948	multifaceted protective effects	2918:2948	multifaceted protective effects against DSS-induced UC in mice	2918:2979	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	4	76	theme	Tibetan	636:642	arg1	resources					651:659	Tibetan herbal resources	636:659	Tibetan herbal resources	636:659	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	14	77	theme	flavonoids	2688:2697	arg1	treatment					2699:2707	LRH flavonoids treatment	2684:2707	LRH flavonoids treatment	2684:2707	The mRNA and proteins expression levels of inflammatory mediators were returned to dynamic balance following LRH flavonoids treatment.
35777121	4	78	from	efficacy	678:685	arg1	UC					805:806	UC	805:806	UC	805:806	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	78	from	efficacy	678:685	arg1	colitis					796:802	dextran sulfate sodium (DSS)-induced ulcerative colitis	748:802	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC)	748:807	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	1	79	theme	medicinal	251:259	arg1	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	79	theme	medicinal	251:259	arg1	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	7	80	theme	histology	1444:1452	arg1	observation					1454:1464	histology observation	1444:1464	histology observation	1444:1464	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	9	81	theme	PI3K/AKT	1847:1854	arg1	pathways					1856:1863	the PI3K/AKT pathways	1843:1863	the PI3K/AKT pathways	1843:1863	Finally, the key regulatory proteins in the PI3K/AKT pathways were measured to investigate their underlying molecular mechanisms.
35777121	8	82	theme	present	1586:1592	arg1	investigation					1594:1606	the present investigation	1582:1606	the present investigation	1582:1606	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	17	83	theme	pathway	3166:3172	arg1	modulation					3139:3148	modulation	3139:3148	modulation of the PI3K/AKT pathway	3139:3172	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	13	84	theme	epithelial	2550:2559	arg1	barrier					2566:2572	the intestinal epithelial cell barrier	2535:2572	the intestinal epithelial cell barrier	2535:2572	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	3	85	theme	medicinal	529:537	arg1	resource					539:546	a medicinal resource	527:546	a medicinal resource	527:546	However, the specific effects of LRH and its mechanism remain unknown, and there is a deficiency of systematic research, leading to the waste of LRH as a medicinal resource.
35777121	8	86	theme	high-throughput	1649:1663	arg1	sequencing					1665:1674	high-throughput sequencing	1649:1674	high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms	1649:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	1	87	theme	in	266:267	arg1	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	87	theme	in	266:267	arg1	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	15	88	theme	microbiome	2796:2805	arg1	recovery					2780:2787	the recovery	2776:2787	the recovery of gut microbiome	2776:2805	16S rDNA sequence analysis indicated that LRH flavonoids promoted the recovery of gut microbiome.
35777121	13	89	theme	animal	2342:2347	arg1	experiments					2349:2359	the animal experiments	2338:2359	the animal experiments	2338:2359	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	1	90	theme	Lonicera	188:195	arg1	LRH					223:225	LRH	223:225	LRH	223:225	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	90	theme	Lonicera	188:195	arg1	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	90	theme	Lonicera	188:195	arg1	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	7	91	theme	disease	1414:1420	arg1	DAI					1438:1440	DAI	1438:1440	DAI	1438:1440	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	91	theme	disease	1414:1420	arg1	index					1431:1435	disease activity index	1414:1435	disease activity index (DAI)	1414:1441	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	5	92	dep	METHODS	996:1002	arg1	used					1095:1098	used	1095:1098	was used for identification and detection of the flavonoid components of LRH	1091:1166	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	15	93	theme	16S	2710:2712	arg1	analysis					2728:2735	16S rDNA sequence analysis	2710:2735	16S rDNA sequence analysis	2710:2735	16S rDNA sequence analysis indicated that LRH flavonoids promoted the recovery of gut microbiome.
35777121	6	94	theme	signaling	1230:1238	arg1	pathways					1240:1247	signaling pathways	1230:1247	signaling pathways	1230:1247	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	6	94	theme	signaling	1230:1238	arg1	Meanwhile					1169:1177	Meanwhile	1169:1177	Meanwhile	1169:1177	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	8	95	theme	V3-V4	1683:1687	arg1	region					1689:1694	the V3-V4 region	1679:1694	the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms	1679:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	1	96	theme	Hook.f.et	206:214	arg1	LRH					223:225	LRH	223:225	LRH	223:225	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	96	theme	Hook.f.et	206:214	arg1	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	96	theme	Hook.f.et	206:214	arg1	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	11	97	theme	common	2103:2108	arg1	targets					2110:2116	their common targets	2097:2116	their common targets	2097:2116	137 of their common targets with UC were further predicted.
35777121	9	98	from	proteins	1831:1838	arg1	pathways					1856:1863	the PI3K/AKT pathways	1843:1863	the PI3K/AKT pathways	1843:1863	Finally, the key regulatory proteins in the PI3K/AKT pathways were measured to investigate their underlying molecular mechanisms.
35777121	4	99	theme	resources	651:659	arg1	utilization					621:631	utilization	621:631	utilization	621:631	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	99	theme	resources	651:659	arg1	development					605:615	the development	601:615	the development	601:615	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	0	100	theme	ulcerative	136:145	arg1	colitis					147:153	ulcerative colitis	136:153	ulcerative colitis	136:153	Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway.
35777121	2	101	theme	excellent	303:311	arg1	activities					363:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	And LRH flavonoids have excellent anti-inflammatory and antioxidant pharmacological activities.
35777121	16	102	theme	LRH	2865:2867	arg1	flavonoids					2869:2878	LRH flavonoids	2865:2878	LRH flavonoids	2865:2878	And the PI3K/AKT pathway was significantly suppressed by LRH flavonoids.
35777121	9	103	theme	molecular	1911:1919	arg1	mechanisms					1921:1930	their underlying molecular mechanisms	1894:1930	their underlying molecular mechanisms	1894:1930	Finally, the key regulatory proteins in the PI3K/AKT pathways were measured to investigate their underlying molecular mechanisms.
35777121	5	104	theme	tandem	1053:1058	arg1	UPLC-MS/MS					1079:1088	UPLC-MS/MS	1079:1088	UPLC-MS/MS	1079:1088	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	104	theme	tandem	1053:1058	arg1	spectrometry					1065:1076	ultra-performance liquid chromatography tandem mass spectrometry	1013:1076	ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)	1013:1089	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	17	105	theme	oxidative	3023:3031	arg1	stress					3033:3038	oxidative stress	3023:3038	oxidative stress	3023:3038	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	8	106	theme	ribosomal	1703:1711	arg1	rDNA					1718:1721	rDNA	1718:1721	rDNA	1718:1721	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	8	106	theme	ribosomal	1703:1711	arg1	DNA					1713:1715	16S ribosomal DNA	1699:1715	16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms	1699:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	6	107	theme	biological	1205:1214	arg1	Meanwhile					1169:1177	Meanwhile	1169:1177	Meanwhile	1169:1177	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	6	107	theme	biological	1205:1214	arg1	functions					1216:1224	biological functions	1205:1224	biological functions	1205:1224	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	10	108	theme	LRH	1955:1957	arg1	components					1969:1978	37 LRH flavonoid components	1952:1978	37 LRH flavonoid components	1952:1978	RESULTS A total of 37 LRH flavonoid components were identified and detected by UPLC-MS/MS, and 12 potential active components were obtained after screening.
35777121	6	109	theme	analysis	1308:1315	arg1	assistance					1273:1282	the assistance	1269:1282	the assistance of network pharmacology analysis	1269:1315	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	18	110	contain	possessed	3221:3229	arg1	flavonoids					3210:3219	LRH flavonoids	3206:3219	LRH flavonoids	3206:3219	This finding demonstrated that LRH flavonoids possessed great potential for becoming an excellent drug for the treatment of UC.
35777121	18	110	contain	possessed	3221:3229	arg2	potential					3237:3245	great potential	3231:3245	great potential for becoming an excellent drug for the treatment of UC	3231:3300	This finding demonstrated that LRH flavonoids possessed great potential for becoming an excellent drug for the treatment of UC.
35777121	14	111	theme	inflammatory	2618:2629	arg1	mediators					2631:2639	inflammatory mediators	2618:2639	inflammatory mediators	2618:2639	The mRNA and proteins expression levels of inflammatory mediators were returned to dynamic balance following LRH flavonoids treatment.
35777121	1	112	dep	in	266:267	arg1	Tibetans					269:276	Tibetans	269:276	Tibetans	269:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	18	113	theme	LRH	3206:3208	arg1	flavonoids					3210:3219	LRH flavonoids	3206:3219	LRH flavonoids	3206:3219	This finding demonstrated that LRH flavonoids possessed great potential for becoming an excellent drug for the treatment of UC.
35777121	6	114	theme	network	1287:1293	arg1	analysis					1308:1315	network pharmacology analysis	1287:1315	network pharmacology analysis	1287:1315	Meanwhile, their potential targets, biological functions and signaling pathways were predicted with the assistance of network pharmacology analysis.
35777121	17	115	theme	epithelial	3051:3060	arg1	function					3070:3077	epithelial barrier function	3051:3077	epithelial barrier function	3051:3077	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	7	116	theme	proteins	1559:1566	arg1	length					1406:1411	colon length	1400:1411	colon length	1400:1411	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	116	theme	proteins	1559:1566	arg1	DAI					1438:1440	DAI	1438:1440	DAI	1438:1440	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	116	theme	proteins	1559:1566	arg1	levels					1485:1490	the expression levels	1470:1490	the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins	1470:1566	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	116	theme	proteins	1559:1566	arg1	index					1431:1435	disease activity index	1414:1435	disease activity index (DAI)	1414:1441	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	116	theme	proteins	1559:1566	arg1	observation					1454:1464	histology observation	1444:1464	histology observation	1444:1464	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	116	theme	proteins	1559:1566	arg1	loss					1394:1397	body weight loss	1382:1397	body weight loss	1382:1397	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	4	117	theme	material	885:892	arg1	basis					894:898	the pharmacodynamic material basis	865:898	the pharmacodynamic material basis of LRH	865:905	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	118	theme	molecular	706:714	arg1	mechanisms					716:725	the underlying molecular mechanisms	691:725	the underlying molecular mechanisms	691:725	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	3	119	theme	research	486:493	arg1	deficiency					461:470	a deficiency	459:470	a deficiency of systematic research	459:493	However, the specific effects of LRH and its mechanism remain unknown, and there is a deficiency of systematic research, leading to the waste of LRH as a medicinal resource.
35777121	15	120	theme	rDNA	2714:2717	arg1	analysis					2728:2735	16S rDNA sequence analysis	2710:2735	16S rDNA sequence analysis	2710:2735	16S rDNA sequence analysis indicated that LRH flavonoids promoted the recovery of gut microbiome.
35777121	4	121	dep	attempt	578:584	arg1	rationalize					589:599	rationalize	589:599	to rationalize the development and utilization of Tibetan herbal resources	586:659	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	8	122	theme	bacterial	1728:1736	arg1	composition					1748:1758	bacterial community composition	1728:1758	bacterial community composition	1728:1758	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	10	123	theme	potential	2031:2039	arg1	components					2048:2057	12 potential active components	2028:2057	12 potential active components	2028:2057	RESULTS A total of 37 LRH flavonoid components were identified and detected by UPLC-MS/MS, and 12 potential active components were obtained after screening.
35777121	7	124	theme	tight	1544:1548	arg1	proteins					1559:1566	tight junction proteins	1544:1566	tight junction proteins	1544:1566	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	16	125	theme	PI3K/AKT	2816:2823	arg1	pathway					2825:2831	the PI3K/AKT pathway	2812:2831	the PI3K/AKT pathway	2812:2831	And the PI3K/AKT pathway was significantly suppressed by LRH flavonoids.
35777121	10	126	theme	components	1969:1978	arg1	total					1943:1947	A total	1941:1947	RESULTS A total of 37 LRH flavonoid components	1933:1978	RESULTS A total of 37 LRH flavonoid components were identified and detected by UPLC-MS/MS, and 12 potential active components were obtained after screening.
35777121	12	127	theme	LRH	2242:2244	arg1	flavonoids					2246:2255	LRH flavonoids	2242:2255	LRH flavonoids	2242:2255	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	14	128	theme	mRNA	2579:2582	arg1	levels					2608:2613	The mRNA and proteins expression levels	2575:2613	The mRNA and proteins expression levels of inflammatory mediators	2575:2639	The mRNA and proteins expression levels of inflammatory mediators were returned to dynamic balance following LRH flavonoids treatment.
35777121	13	129	theme	body	2442:2445	arg1	loss					2454:2457	the body weight loss	2438:2457	the body weight loss	2438:2457	In the animal experiments, LRH flavonoids could significantly attenuate UC as demonstrated by reducing the body weight loss and DAI, restoring colon length, decreasing oxidative stress, and improving the intestinal epithelial cell barrier.
35777121	3	130	theme	specific	388:395	arg1	effects					397:403	the specific effects	384:403	the specific effects of LRH	384:410	However, the specific effects of LRH and its mechanism remain unknown, and there is a deficiency of systematic research, leading to the waste of LRH as a medicinal resource.
35777121	10	131	dep	RESULTS	1933:1939	arg1	total					1943:1947	A total	1941:1947	RESULTS A total of 37 LRH flavonoid components	1933:1978	RESULTS A total of 37 LRH flavonoid components were identified and detected by UPLC-MS/MS, and 12 potential active components were obtained after screening.
35777121	12	132	theme	molecular	2194:2202	arg1	experiments					2212:2222	molecular docking experiments	2194:2222	molecular docking experiments	2194:2222	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	5	133	used	used	1095:1098	arg2	spectrometry					1065:1076	ultra-performance liquid chromatography tandem mass spectrometry	1013:1076	ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS)	1013:1089	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	5	133	used	used	1095:1098	arg2	UPLC-MS/MS					1079:1088	UPLC-MS/MS	1079:1088	UPLC-MS/MS	1079:1088	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	8	134	theme	Illumina	1777:1784	arg1	platforms					1792:1800	Illumina MiSeq platforms	1777:1800	Illumina MiSeq platforms	1777:1800	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	17	135	theme	CONCLUSIONS	2881:2891	arg1	flavonoids					2897:2906	CONCLUSIONS LRH flavonoids	2881:2906	CONCLUSIONS LRH flavonoids	2881:2906	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	12	136	theme	pathway	2162:2168	arg1	analysis					2181:2188	KEGG pathway enrichment analysis	2157:2188	KEGG pathway enrichment analysis	2157:2188	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	0	137	theme	Thoms	28:32	arg1	flavonoids					34:43	Lonicera rupicola Hook.f.et Thoms flavonoids	0:43	Lonicera rupicola Hook.f.et Thoms flavonoids	0:43	Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway.
35777121	17	138	from	effects	2942:2948	arg1	mice					2976:2979	mice	2976:2979	mice	2976:2979	CONCLUSIONS LRH flavonoids exhibited multifaceted protective effects against DSS-induced UC in mice through mitigating colon inflammation and oxidative stress, restoring epithelial barrier function, and improving the gut microenvironment potentially through modulation of the PI3K/AKT pathway.
35777121	4	139	theme	flavonoids	734:743	arg1	efficacy					678:685	the therapeutic efficacy	662:685	the therapeutic efficacy	662:685	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	139	theme	flavonoids	734:743	arg1	mechanisms					716:725	the underlying molecular mechanisms	691:725	the underlying molecular mechanisms	691:725	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	3	140	theme	systematic	475:484	arg1	research					486:493	systematic research	475:493	systematic research	475:493	However, the specific effects of LRH and its mechanism remain unknown, and there is a deficiency of systematic research, leading to the waste of LRH as a medicinal resource.
35777121	7	141	theme	RNA	1529:1531	arg1	length					1406:1411	colon length	1400:1411	colon length	1400:1411	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	141	theme	RNA	1529:1531	arg1	DAI					1438:1440	DAI	1438:1440	DAI	1438:1440	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	141	theme	RNA	1529:1531	arg1	levels					1485:1490	the expression levels	1470:1490	the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins	1470:1566	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	141	theme	RNA	1529:1531	arg1	index					1431:1435	disease activity index	1414:1435	disease activity index (DAI)	1414:1441	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	141	theme	RNA	1529:1531	arg1	observation					1454:1464	histology observation	1444:1464	histology observation	1444:1464	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	7	141	theme	RNA	1529:1531	arg1	loss					1394:1397	body weight loss	1382:1397	body weight loss	1382:1397	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	4	142	theme	drugs	965:969	arg1	discovery					948:956	the discovery	944:956	the discovery of new drugs for the treatment of UC	944:993	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	0	143	theme	inflammatory	70:81	arg1	responses					83:91	dysregulated inflammatory responses	57:91	dysregulated inflammatory responses	57:91	Lonicera rupicola Hook.f.et Thoms flavonoids ameliorated dysregulated inflammatory responses, intestinal barrier, and gut microbiome in ulcerative colitis via PI3K/AKT pathway.
35777121	18	144	theme	excellent	3263:3271	arg1	drug					3273:3276	an excellent drug	3260:3276	an excellent drug for the treatment of UC	3260:3300	This finding demonstrated that LRH flavonoids possessed great potential for becoming an excellent drug for the treatment of UC.
35777121	4	145	from	mechanisms	716:725	arg1	UC					805:806	UC	805:806	UC	805:806	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	145	from	mechanisms	716:725	arg1	colitis					796:802	dextran sulfate sodium (DSS)-induced ulcerative colitis	748:802	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC)	748:807	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	5	146	theme	flavonoid	1140:1148	arg1	components					1150:1159	the flavonoid components	1136:1159	the flavonoid components of LRH	1136:1166	METHODS Firstly, ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was used for identification and detection of the flavonoid components of LRH.
35777121	7	147	theme	inflammatory	1495:1506	arg1	mediators					1508:1516	inflammatory mediators	1495:1516	inflammatory mediators	1495:1516	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	18	148	theme	UC	3299:3300	arg1	treatment					3286:3294	the treatment	3282:3294	the treatment of UC	3282:3300	This finding demonstrated that LRH flavonoids possessed great potential for becoming an excellent drug for the treatment of UC.
35777121	7	149	theme	weight	1387:1392	arg1	loss					1394:1397	body weight loss	1382:1397	body weight loss	1382:1397	Subsequently, pharmacological efficacy of LRH were evaluated by body weight loss, colon length, disease activity index (DAI), histology observation and the expression levels of inflammatory mediators, messenger RNA (mRNA) and tight junction proteins.
35777121	9	150	theme	key	1816:1818	arg1	proteins					1831:1838	the key regulatory proteins	1812:1838	the key regulatory proteins in the PI3K/AKT pathways	1812:1863	Finally, the key regulatory proteins in the PI3K/AKT pathways were measured to investigate their underlying molecular mechanisms.
35777121	2	151	theme	antioxidant	335:345	arg1	activities					363:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	excellent anti-inflammatory and antioxidant pharmacological activities	303:372	And LRH flavonoids have excellent anti-inflammatory and antioxidant pharmacological activities.
35777121	8	152	theme	gut	1630:1632	arg1	microbiome					1634:1643	the gut microbiome	1626:1643	the gut microbiome	1626:1643	Moreover, in the present investigation, we also profiled the gut microbiome via high-throughput sequencing of the V3-V4 region of 16S ribosomal DNA (rDNA) for bacterial community composition and diversity by Illumina MiSeq platforms.
35777121	1	153	used	used	231:234	arg2	LRH					223:225	LRH	223:225	LRH	223:225	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	153	used	used	231:234	arg2	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	153	used	used	231:234	arg2	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	12	154	theme	multi-component-multi-target-multi-pathway	2290:2331	arg1	"					2332:2332	"multi-component-multi-target-multi-pathway"	2289:2332	"multi-component-multi-target-multi-pathway"	2289:2332	GO and KEGG pathway enrichment analysis and molecular docking experiments demonstrated that LRH flavonoids could interfere with UC through "multi-component-multi-target-multi-pathway".
35777121	4	155	theme	-induced	776:783	arg1	UC					805:806	UC	805:806	UC	805:806	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	4	155	theme	-induced	776:783	arg1	colitis					796:802	dextran sulfate sodium (DSS)-induced ulcerative colitis	748:802	dextran sulfate sodium (DSS)-induced ulcerative colitis (UC)	748:807	PURPOSE In this study, in an attempt to rationalize the development and utilization of Tibetan herbal resources, the therapeutic efficacy and the underlying molecular mechanisms of LRH flavonoids on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) were investigated, establishing the favorable basis for the pharmacodynamic material basis of LRH and providing a scientific basis for the discovery of new drugs for the treatment of UC.
35777121	3	156	theme	LRH	408:410	arg1	mechanism					420:428	its mechanism	416:428	its mechanism	416:428	However, the specific effects of LRH and its mechanism remain unknown, and there is a deficiency of systematic research, leading to the waste of LRH as a medicinal resource.
35777121	3	156	theme	LRH	408:410	arg1	effects					397:403	the specific effects	384:403	the specific effects of LRH	384:410	However, the specific effects of LRH and its mechanism remain unknown, and there is a deficiency of systematic research, leading to the waste of LRH as a medicinal resource.
35777121	1	157	theme	customary	241:249	arg1	herb					261:264	a customary medicinal herb	239:264	a customary medicinal herb in Tibetans	239:276	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
35777121	1	157	theme	customary	241:249	arg1	Thoms					216:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms	177:220	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH)	177:226	BACKGROUND Lonicera rupicola Hook.f.et Thoms (LRH) is used as a customary medicinal herb in Tibetans.
36255673	6	0	dep	specific	698:705	arg1	cell					683:686	cell	683:686	cell	683:686	Their expression is cell or tissue specific and developmentally regulated.
36255673	8	1	theme	enzyme	986:991	arg1	deficits					993:1000	glycosylation enzyme deficits	972:1000	glycosylation enzyme deficits	972:1000	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	7	2	theme	structural	751:760	arg1	diversity					762:770	their structural diversity	745:770	their structural diversity	745:770	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	4	3	theme	acid	525:528	arg1	lipids					486:491	glucosylated lipids	473:491	glucosylated lipids of cholesterol and phosphatidic acid	473:528	Recent careful analyses by MS have identified glucosylated lipids of cholesterol and phosphatidic acid.
36255673	3	4	mod	modified	303:310	arg1	lipid					283:287	Membrane lipid	274:287	Membrane lipid	274:287	Membrane lipid, ceramide, is modified by the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide.
36255673	3	4	mod	modified	303:310	arg3	addition					319:326	the addition	315:326	the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide	315:424	Membrane lipid, ceramide, is modified by the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide.
36255673	3	4	mod	modified	303:310	arg1	ceramide					290:297	ceramide	290:297	ceramide	290:297	Membrane lipid, ceramide, is modified by the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide.
36255673	5	5	theme	plasma	616:621	arg1	membrane					623:630	the plasma membrane	612:630	the plasma membrane	612:630	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	7	6	theme	cell-cell	892:900	arg1	interactions					902:913	cell-cell interactions	892:913	cell-cell interactions	892:913	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	6	theme	cell-cell	892:900	arg1	transport					853:861	membrane transport	844:861	membrane transport	844:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	6	theme	cell-cell	892:900	arg1	processes					826:834	biological processes	815:834	biological processes such as membrane transport	815:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	3	7	theme	galactose	349:357	arg1	addition					319:326	the addition	315:326	the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide	315:424	Membrane lipid, ceramide, is modified by the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide.
36255673	8	8	theme	critical	1074:1081	arg1	roles					1083:1087	critical roles	1074:1087	critical roles	1074:1087	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	5	9	theme	membrane	623:630	arg1	surface					601:607	the outer surface	591:607	the outer surface of the plasma membrane	591:630	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	7	10	theme	stress	874:879	arg1	transport					853:861	membrane transport	844:861	membrane transport	844:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	10	theme	stress	874:879	arg1	processes					826:834	biological processes	815:834	biological processes such as membrane transport	815:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	10	theme	stress	874:879	arg1	responses					881:889	metabolic stress responses	864:889	metabolic stress responses	864:889	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	8	11	theme	lipids	1030:1035	arg1	modification					1014:1025	modification	1014:1025	modification of lipids and proteins with carbohydrates	1014:1067	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	4	12	theme	glucosylated	473:484	arg1	lipids					486:491	glucosylated lipids	473:491	glucosylated lipids of cholesterol and phosphatidic acid	473:528	Recent careful analyses by MS have identified glucosylated lipids of cholesterol and phosphatidic acid.
36255673	8	13	theme	human	1092:1096	arg1	health					1098:1103	human health	1092:1103	human health	1092:1103	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	3	14	theme	glucose	338:344	arg1	addition					319:326	the addition	315:326	the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide	315:424	Membrane lipid, ceramide, is modified by the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide.
36255673	4	15	theme	cholesterol	496:506	arg1	lipids					486:491	glucosylated lipids	473:491	glucosylated lipids of cholesterol and phosphatidic acid	473:528	Recent careful analyses by MS have identified glucosylated lipids of cholesterol and phosphatidic acid.
36255673	1	16	theme	O-linked	84:91	arg1	N-acetylglucosamine					116:134	O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose	84:172	N-acetylglucosamine	116:134	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	5	17	theme	extracellular	642:654	arg1	space					656:660	the extracellular space	638:660	the extracellular space	638:660	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	5	18	theme	outer	595:599	arg1	surface					601:607	the outer surface	591:607	the outer surface of the plasma membrane	591:630	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	1	19	theme	N-acetylgalactosamine	93:113	arg1	N-acetylglucosamine					116:134	O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose	84:172	N-acetylglucosamine	116:134	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	7	20	theme	metabolic	864:872	arg1	transport					853:861	membrane transport	844:861	membrane transport	844:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	20	theme	metabolic	864:872	arg1	processes					826:834	biological processes	815:834	biological processes such as membrane transport	815:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	20	theme	metabolic	864:872	arg1	responses					881:889	metabolic stress responses	864:889	metabolic stress responses	864:889	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	21	theme	biological	815:824	arg1	interactions					902:913	cell-cell interactions	892:913	cell-cell interactions	892:913	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	21	theme	biological	815:824	arg1	transport					853:861	membrane transport	844:861	membrane transport	844:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	21	theme	biological	815:824	arg1	responses					881:889	metabolic stress responses	864:889	metabolic stress responses	864:889	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	21	theme	biological	815:824	arg1	processes					826:834	biological processes	815:834	biological processes such as membrane transport	815:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	0	22	theme	Nervous	45:51	arg1	System					53:58	the Nervous System	41:58	the Nervous System	41:58	Synthesis of O-Linked Glycoconjugates in the Nervous System.
36255673	0	23	theme	Glycoconjugates	22:36	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of O-Linked Glycoconjugates in the Nervous System.	0:59	Synthesis of O-Linked Glycoconjugates in the Nervous System.
36255673	1	24	contain	carrying	75:82	arg2	N-acetylglucosamine					116:134	O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose	84:172	N-acetylglucosamine	116:134	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	1	24	contain	carrying	75:82	arg1	Glycoproteins					61:73	Glycoproteins	61:73	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose	61:172	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	7	25	dep	and	915:917	arg1	so					919:920	so	919:920	so	919:920	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	1	26	theme	nervous	191:197	arg1	system					199:204	the nervous system	187:204	the nervous system	187:204	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	0	27	theme	O-Linked	13:20	arg1	Glycoconjugates					22:36	O-Linked Glycoconjugates	13:36	O-Linked Glycoconjugates	13:36	Synthesis of O-Linked Glycoconjugates in the Nervous System.
36255673	1	28	located	found	178:182	arg1	system					199:204	the nervous system	187:204	the nervous system	187:204	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	1	28	located	found	178:182	arg2	Glycoproteins					61:73	Glycoproteins	61:73	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose	61:172	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	7	29	theme	membrane	844:851	arg1	transport					853:861	membrane transport	844:861	membrane transport	844:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	5	30	link	O-linked	537:544	arg1	residues					559:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	8	31	theme	nervous	1124:1130	arg1	systems					1132:1138	the nervous systems	1120:1138	the nervous systems	1120:1138	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	2	32	theme	distinct	234:241	arg1	enzymes					257:263	distinct glycosylation enzymes	234:263	distinct glycosylation enzymes as well	234:271	Lipids are glycosylated by distinct glycosylation enzymes as well.
36255673	3	33	theme	Membrane	274:281	arg1	lipid					283:287	Membrane lipid	274:287	Membrane lipid	274:287	Membrane lipid, ceramide, is modified by the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide.
36255673	3	33	theme	Membrane	274:281	arg1	ceramide					290:297	ceramide	290:297	ceramide	290:297	Membrane lipid, ceramide, is modified by the addition of either glucose or galactose to form glycosphingolipid, galactosylceramide, or glucosylceramide.
36255673	8	34	theme	human	941:945	arg1	diseases					947:954	human diseases	941:954	human diseases associated with glycosylation enzyme deficits	941:1000	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	7	35	theme	processes	826:834	arg1	on					922:923	on	922:923	on	922:923	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	35	theme	processes	826:834	arg1	transport					853:861	membrane transport	844:861	membrane transport	844:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	35	theme	processes	826:834	arg1	variety					804:810	a variety	802:810	a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on	802:923	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	35	theme	processes	826:834	arg1	interactions					902:913	cell-cell interactions	892:913	cell-cell interactions	892:913	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	35	theme	processes	826:834	arg1	processes					826:834	biological processes	815:834	biological processes such as membrane transport	815:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	35	theme	processes	826:834	arg1	responses					881:889	metabolic stress responses	864:889	metabolic stress responses	864:889	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	2	36	gly	glycosylated	218:229	arg1	Lipids					207:212	Lipids	207:212	Lipids	207:212	Lipids are glycosylated by distinct glycosylation enzymes as well.
36255673	7	37	theme	important	783:791	arg1	roles					793:797	important roles	783:797	important roles	783:797	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	4	38	theme	Recent	427:432	arg1	analyses					442:449	Recent careful analyses	427:449	Recent careful analyses by MS	427:455	Recent careful analyses by MS have identified glucosylated lipids of cholesterol and phosphatidic acid.
36255673	8	39	theme	proteins	1041:1048	arg1	modification					1014:1025	modification	1014:1025	modification of lipids and proteins with carbohydrates	1014:1067	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	8	40	theme	diseases	947:954	arg1	Discoveries					926:936	Discoveries	926:936	Discoveries of human diseases associated with glycosylation enzyme deficits	926:1000	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	5	41	theme	carbohydrate	546:557	arg1	residues					559:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	8	42	theme	glycosylation	972:984	arg1	deficits					993:1000	glycosylation enzyme deficits	972:1000	glycosylation enzyme deficits	972:1000	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	5	43	located	found	572:576	arg1	surface					601:607	the outer surface	591:607	the outer surface of the plasma membrane	591:630	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	5	43	located	found	572:576	arg2	residues					559:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	4	44	theme	careful	434:440	arg1	analyses					442:449	Recent careful analyses	427:449	Recent careful analyses by MS	427:455	Recent careful analyses by MS have identified glucosylated lipids of cholesterol and phosphatidic acid.
36255673	5	45	theme	O-linked	537:544	arg1	residues					559:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues	531:566	These O-linked carbohydrate residues are found primarily on the outer surface of the plasma membrane or in the extracellular space.
36255673	1	46	dep	N-acetylglucosamine	116:134	arg1	fucose					146:151	fucose	146:151	fucose	146:151	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	1	46	dep	N-acetylglucosamine	116:134	arg1	mannose					137:143	mannose	137:143	mannose	137:143	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	1	46	dep	N-acetylglucosamine	116:134	arg1	glucose					154:160	glucose	154:160	glucose	154:160	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	1	46	dep	N-acetylglucosamine	116:134	arg1	xylose					167:172	xylose	167:172	xylose	167:172	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
36255673	7	47	theme	on	922:923	arg1	on					922:923	on	922:923	on	922:923	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	47	theme	on	922:923	arg1	transport					853:861	membrane transport	844:861	membrane transport	844:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	47	theme	on	922:923	arg1	variety					804:810	a variety	802:810	a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on	802:923	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	47	theme	on	922:923	arg1	interactions					902:913	cell-cell interactions	892:913	cell-cell interactions	892:913	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	47	theme	on	922:923	arg1	processes					826:834	biological processes	815:834	biological processes such as membrane transport	815:861	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	7	47	theme	on	922:923	arg1	responses					881:889	metabolic stress responses	864:889	metabolic stress responses	864:889	Due to their structural diversity, they play important roles in a variety of biological processes such as membrane transport, metabolic stress responses, cell-cell interactions and so on.
36255673	8	48	with	modification	1014:1025	arg1	carbohydrates					1055:1067	carbohydrates	1055:1067	carbohydrates	1055:1067	Discoveries of human diseases associated with glycosylation enzyme deficits have proved modification of lipids and proteins with carbohydrates play critical roles in human health and disease in the nervous systems.
36255673	4	49	theme	phosphatidic	512:523	arg1	acid					525:528	phosphatidic acid	512:528	phosphatidic acid	512:528	Recent careful analyses by MS have identified glucosylated lipids of cholesterol and phosphatidic acid.
36255673	2	50	theme	glycosylation	243:255	arg1	enzymes					257:263	distinct glycosylation enzymes	234:263	distinct glycosylation enzymes as well	234:271	Lipids are glycosylated by distinct glycosylation enzymes as well.
36255673	0	51	from	Synthesis	0:8	arg1	System					53:58	the Nervous System	41:58	the Nervous System	41:58	Synthesis of O-Linked Glycoconjugates in the Nervous System.
36255673	1	52	link	O-linked	84:91	arg1	N-acetylglucosamine					116:134	O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose	84:172	N-acetylglucosamine	116:134	Glycoproteins carrying O-linked N-acetylgalactosamine, N-acetylglucosamine, mannose, fucose, glucose, and xylose are found in the nervous system.
35335136	5	0	theme	optimized	725:733	arg1	parameters					751:760	kinetics parameters	742:760	kinetics parameters	742:760	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	5	0	theme	optimized	725:733	arg1	set					735:737	an optimized set	722:737	an optimized set of kinetics parameters for each enzyme	722:776	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	6	1	theme	glycans	866:872	arg1	abundance					838:846	the abundance	834:846	the abundance of more processed glycans	834:872	Using this optimized model we showed that the abundance of more processed glycans could be simulated compared to observed abundance, while predicting the abundance of glycans earlier in the pathway was less accurate.
35335136	6	1	theme	glycans	866:872	arg1	simulated					883:891	simulated	883:891	simulated	883:891	Using this optimized model we showed that the abundance of more processed glycans could be simulated compared to observed abundance, while predicting the abundance of glycans earlier in the pathway was less accurate.
35335136	1	2	theme	first	156:160	arg1	model					175:179	the first mathematical model	152:179	the first mathematical model for N-glycan biosynthesis	152:205	Glycan biosynthesis simulation research has progressed remarkably since 1997, when the first mathematical model for N-glycan biosynthesis was proposed.
35335136	4	3	theme	cell	578:581	arg1	lines					583:587	the CHO transfected cell lines	558:587	the CHO transfected cell lines	558:587	The aim was to develop a model that encapsulated all the enzymes in the CHO transfected cell lines.
35335136	5	4	theme	profiles	682:689	arg1	set					668:670	a smaller set	658:670	a smaller set	658:670	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	5	4	theme	profiles	682:689	arg1	profiles					682:689	glycan profiles	675:689	glycan profiles	675:689	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	0	5	from	Modeling	14:21	arg1	Cells					62:66	CHO Cells	58:66	CHO Cells	58:66	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells.
35335136	7	6	theme	O-linked	1069:1076	arg1	glycosylation					1078:1090	O-linked glycosylation	1069:1090	O-linked glycosylation	1069:1090	The data generated show that for the accurate prediction of O-linked glycosylation, additional factors need to be incorporated into the model to better reflect the experimental conditions.
35335136	1	7	theme	mathematical	162:173	arg1	model					175:179	the first mathematical model	152:179	the first mathematical model for N-glycan biosynthesis	152:205	Glycan biosynthesis simulation research has progressed remarkably since 1997, when the first mathematical model for N-glycan biosynthesis was proposed.
35335136	4	8	theme	transfected	566:576	arg1	lines					583:587	the CHO transfected cell lines	558:587	the CHO transfected cell lines	558:587	The aim was to develop a model that encapsulated all the enzymes in the CHO transfected cell lines.
35335136	7	9	theme	accurate	1046:1053	arg1	prediction					1055:1064	the accurate prediction	1042:1064	the accurate prediction of O-linked glycosylation	1042:1090	The data generated show that for the accurate prediction of O-linked glycosylation, additional factors need to be incorporated into the model to better reflect the experimental conditions.
35335136	5	10	theme	parameters	751:760	arg1	parameters					751:760	kinetics parameters	742:760	kinetics parameters	742:760	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	5	10	theme	parameters	751:760	arg1	set					735:737	an optimized set	722:737	an optimized set of kinetics parameters for each enzyme	722:776	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	2	11	theme	reverse	325:331	arg1	directions					333:342	both forward and reverse directions	308:342	both forward and reverse directions	308:342	An O-glycan model has also been developed to predict O-glycan biosynthesis pathways in both forward and reverse directions.
35335136	5	12	theme	computational	597:609	arg1	restrictions					617:628	computational power restrictions	597:628	computational power restrictions	597:628	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	5	13	theme	power	611:615	arg1	restrictions					617:628	computational power restrictions	597:628	computational power restrictions	597:628	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	1	14	theme	Glycan	69:74	arg1	research					100:107	Glycan biosynthesis simulation research	69:107	Glycan biosynthesis simulation research	69:107	Glycan biosynthesis simulation research has progressed remarkably since 1997, when the first mathematical model for N-glycan biosynthesis was proposed.
35335136	1	15	theme	N-glycan	185:192	arg1	biosynthesis					194:205	N-glycan biosynthesis	185:205	N-glycan biosynthesis	185:205	Glycan biosynthesis simulation research has progressed remarkably since 1997, when the first mathematical model for N-glycan biosynthesis was proposed.
35335136	5	16	theme	kinetics	742:749	arg1	parameters					751:760	kinetics parameters	742:760	kinetics parameters	742:760	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	7	17	theme	glycosylation	1078:1090	arg1	prediction					1055:1064	the accurate prediction	1042:1064	the accurate prediction of O-linked glycosylation	1042:1090	The data generated show that for the accurate prediction of O-linked glycosylation, additional factors need to be incorporated into the model to better reflect the experimental conditions.
35335136	7	18	theme	additional	1093:1102	arg1	factors					1104:1110	additional factors	1093:1110	additional factors	1093:1110	The data generated show that for the accurate prediction of O-linked glycosylation, additional factors need to be incorporated into the model to better reflect the experimental conditions.
35335136	1	19	theme	biosynthesis	76:87	arg1	research					100:107	Glycan biosynthesis simulation research	69:107	Glycan biosynthesis simulation research	69:107	Glycan biosynthesis simulation research has progressed remarkably since 1997, when the first mathematical model for N-glycan biosynthesis was proposed.
35335136	1	20	theme	simulation	89:98	arg1	research					100:107	Glycan biosynthesis simulation research	69:107	Glycan biosynthesis simulation research	69:107	Glycan biosynthesis simulation research has progressed remarkably since 1997, when the first mathematical model for N-glycan biosynthesis was proposed.
35335136	3	21	theme	cells	409:413	arg1	profiles					393:400	O-glycan profiles	384:400	O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases	384:487	In this work, we started with a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases.
35335136	0	22	theme	Computational	0:12	arg1	Modeling					14:21	Computational Modeling	0:21	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells	0:66	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells.
35335136	0	23	theme	O-Linked	26:33	arg1	Biosynthesis					42:53	O-Linked Glycan Biosynthesis	26:53	O-Linked Glycan Biosynthesis	26:53	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells.
35335136	5	24	theme	glycan	675:680	arg1	profiles					682:689	glycan profiles	675:689	glycan profiles	675:689	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	2	25	theme	forward	313:319	arg1	directions					333:342	both forward and reverse directions	308:342	both forward and reverse directions	308:342	An O-glycan model has also been developed to predict O-glycan biosynthesis pathways in both forward and reverse directions.
35335136	4	26	from	enzymes	547:553	arg1	lines					583:587	the CHO transfected cell lines	558:587	the CHO transfected cell lines	558:587	The aim was to develop a model that encapsulated all the enzymes in the CHO transfected cell lines.
35335136	4	27	theme	CHO	562:564	arg1	lines					583:587	the CHO transfected cell lines	558:587	the CHO transfected cell lines	558:587	The aim was to develop a model that encapsulated all the enzymes in the CHO transfected cell lines.
35335136	2	28	theme	biosynthesis	283:294	arg1	pathways					296:303	O-glycan biosynthesis pathways	274:303	O-glycan biosynthesis pathways in both forward and reverse directions	274:342	An O-glycan model has also been developed to predict O-glycan biosynthesis pathways in both forward and reverse directions.
35335136	3	29	theme	glycosyltransferases	468:487	arg1	combinations					452:463	various combinations	444:463	various combinations of glycosyltransferases	444:487	In this work, we started with a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases.
35335136	0	30	theme	Biosynthesis	42:53	arg1	Modeling					14:21	Computational Modeling	0:21	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells	0:66	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells.
35335136	6	31	theme	observed	905:912	arg1	abundance					914:922	observed abundance	905:922	observed abundance	905:922	Using this optimized model we showed that the abundance of more processed glycans could be simulated compared to observed abundance, while predicting the abundance of glycans earlier in the pathway was less accurate.
35335136	3	32	theme	various	444:450	arg1	combinations					452:463	various combinations	444:463	various combinations of glycosyltransferases	444:487	In this work, we started with a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases.
35335136	0	33	theme	Glycan	35:40	arg1	Biosynthesis					42:53	O-Linked Glycan Biosynthesis	26:53	O-Linked Glycan Biosynthesis	26:53	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells.
35335136	3	34	theme	CHO	405:407	arg1	cells					409:413	CHO cells	405:413	CHO cells transiently transfected with various combinations of glycosyltransferases	405:487	In this work, we started with a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases.
35335136	7	35	link	O-linked	1069:1076	arg1	glycosylation					1078:1090	O-linked glycosylation	1069:1090	O-linked glycosylation	1069:1090	The data generated show that for the accurate prediction of O-linked glycosylation, additional factors need to be incorporated into the model to better reflect the experimental conditions.
35335136	3	36	theme	O-glycan	384:391	arg1	profiles					393:400	O-glycan profiles	384:400	O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases	384:487	In this work, we started with a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases.
35335136	6	37	theme	optimized	803:811	arg1	model					813:817	this optimized model	798:817	this optimized model	798:817	Using this optimized model we showed that the abundance of more processed glycans could be simulated compared to observed abundance, while predicting the abundance of glycans earlier in the pathway was less accurate.
35335136	3	38	theme	profiles	393:400	arg1	set					377:379	a set	375:379	a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases	375:487	In this work, we started with a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases.
35335136	3	38	theme	profiles	393:400	arg1	profiles					393:400	O-glycan profiles	384:400	O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases	384:487	In this work, we started with a set of O-glycan profiles of CHO cells transiently transfected with various combinations of glycosyltransferases.
35335136	6	39	dep	showed	822:827	arg1	Using					792:796	Using	792:796	Using this optimized model	792:817	Using this optimized model we showed that the abundance of more processed glycans could be simulated compared to observed abundance, while predicting the abundance of glycans earlier in the pathway was less accurate.
35335136	5	40	theme	smaller	660:666	arg1	set					668:670	a smaller set	658:670	a smaller set	658:670	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	5	40	theme	smaller	660:666	arg1	profiles					682:689	glycan profiles	675:689	glycan profiles	675:689	Due to computational power restrictions, we were forced to focus on a smaller set of glycan profiles, where we were able to propose an optimized set of kinetics parameters for each enzyme in the model.
35335136	6	41	theme	processed	856:864	arg1	glycans					866:872	more processed glycans	851:872	more processed glycans	851:872	Using this optimized model we showed that the abundance of more processed glycans could be simulated compared to observed abundance, while predicting the abundance of glycans earlier in the pathway was less accurate.
35335136	2	42	theme	O-glycan	224:231	arg1	model					233:237	An O-glycan model	221:237	An O-glycan model	221:237	An O-glycan model has also been developed to predict O-glycan biosynthesis pathways in both forward and reverse directions.
35335136	2	43	theme	O-glycan	274:281	arg1	pathways					296:303	O-glycan biosynthesis pathways	274:303	O-glycan biosynthesis pathways in both forward and reverse directions	274:342	An O-glycan model has also been developed to predict O-glycan biosynthesis pathways in both forward and reverse directions.
35335136	7	44	theme	experimental	1173:1184	arg1	conditions					1186:1195	the experimental conditions	1169:1195	the experimental conditions	1169:1195	The data generated show that for the accurate prediction of O-linked glycosylation, additional factors need to be incorporated into the model to better reflect the experimental conditions.
35335136	2	45	from	pathways	296:303	arg1	directions					333:342	both forward and reverse directions	308:342	both forward and reverse directions	308:342	An O-glycan model has also been developed to predict O-glycan biosynthesis pathways in both forward and reverse directions.
35335136	0	46	theme	CHO	58:60	arg1	Cells					62:66	CHO Cells	58:66	CHO Cells	58:66	Computational Modeling of O-Linked Glycan Biosynthesis in CHO Cells.
35335136	6	47	theme	glycans	959:965	arg1	abundance					946:954	the abundance	942:954	the abundance of glycans	942:965	Using this optimized model we showed that the abundance of more processed glycans could be simulated compared to observed abundance, while predicting the abundance of glycans earlier in the pathway was less accurate.
36149391	6	0	contain	has	1066:1068	arg2	conductivity					1086:1097	a lower thermal conductivity	1070:1097	a lower thermal conductivity	1070:1097	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	6	0	contain	has	1066:1068	arg1	it					1063:1064	it	1063:1064	it	1063:1064	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	4	1	theme	carbonized	699:708	arg1	wood					710:713	the carbonized wood	695:713	the carbonized wood	695:713	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	0	2	with	Evaporator	39:48	arg1	Scaffolding					55:65	Scaffolding	55:65	Scaffolding	55:65	Highly Effective Multifunctional Solar Evaporator with Scaffolding Structured Carbonized Wood and Biohydrogel.
36149391	2	3	theme	L.	361:362	arg1	wood					330:333	composite carbonized wood	309:333	composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel	309:384	In this study, a solar evaporator was prepared by assembling composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel.
36149391	6	4	theme	thermal	1078:1084	arg1	conductivity					1086:1097	a lower thermal conductivity	1070:1097	a lower thermal conductivity	1070:1097	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	6	5	theme	konjac	993:998	arg1	glucomannan					1000:1010	konjac glucomannan and sodium alginate polysaccharides	993:1046	glucomannan	1000:1010	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	3	6	theme	wood	466:469	arg1	structure					449:457	the scaffolding structure	433:457	the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force	433:670	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	7	7	theme	sun	1368:1370	arg1	illumination					1372:1383	2 sun illumination	1366:1383	2 sun illumination	1366:1383	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	6	8	with	polysaccharides	1032:1046	arg1	Ca2+					1053:1056	Ca2+	1053:1056	Ca2+	1053:1056	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	5	9	theme	ultraviolet	924:934	arg1	bands					942:946	the infrared and ultraviolet light bands	907:946	bands	942:946	Moreover, the addition of MXene can improve the light absorbance, especially for the infrared and ultraviolet light bands.
36149391	3	10	theme	ordered	526:532	arg1	structure					546:554	the loose and ordered scaffolding structure	512:554	the loose and ordered scaffolding structure of the carbonized wood	512:577	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	3	11	theme	carbonized	489:498	arg1	wood					500:503	composite carbonized wood	479:503	composite carbonized wood	479:503	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	2	12	theme	carbonized	319:328	arg1	wood					330:333	composite carbonized wood	309:333	composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel	309:384	In this study, a solar evaporator was prepared by assembling composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel.
36149391	7	13	contain	has	1276:1278	arg2	rate					1295:1298	an evaporation rate	1280:1298	an evaporation rate of 3.71 kg·m-2·h-1	1280:1317	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	7	13	contain	has	1276:1278	arg2	efficiency					1338:1347	an evaporation efficiency	1323:1347	an evaporation efficiency of 129.64%	1323:1358	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	7	13	contain	has	1276:1278	arg1	evaporator					1265:1274	This solar evaporator	1254:1274	This solar evaporator	1254:1274	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	6	14	theme	lower	1072:1076	arg1	conductivity					1086:1097	a lower thermal conductivity	1070:1097	a lower thermal conductivity	1070:1097	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	2	15	theme	solar	265:269	arg1	evaporator					271:280	a solar evaporator	263:280	a solar evaporator	263:280	In this study, a solar evaporator was prepared by assembling composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel.
36149391	1	16	theme	renewable	176:184	arg1	approach					186:193	a renewable approach	174:193	a renewable approach to deal with energy crisis and fresh water shortage	174:245	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	1	16	theme	renewable	176:184	arg1	evaporator					119:128	A solar evaporator	111:128	A solar evaporator that utilizes solar radiation energy	111:165	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	7	17	theme	mV	1445:1446	arg1	voltage					1430:1436	an open-circuit voltage	1414:1436	an open-circuit voltage of 1.8 mV	1414:1446	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	3	18	theme	loose	516:520	arg1	structure					546:554	the loose and ordered scaffolding structure	512:554	the loose and ordered scaffolding structure of the carbonized wood	512:577	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	0	19	theme	Effective	7:15	arg1	Evaporator					39:48	Highly Effective Multifunctional Solar Evaporator	0:48	Highly Effective Multifunctional Solar Evaporator with Scaffolding	0:65	Highly Effective Multifunctional Solar Evaporator with Scaffolding Structured Carbonized Wood and Biohydrogel.
36149391	4	20	contain	has	715:717	arg2	energy					732:737	high binding energy	719:737	high binding energy with water molecules	719:758	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	4	20	contain	has	715:717	arg1	MXene					677:681	The MXene	673:681	The MXene adsorbed in the carbonized wood	673:713	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	7	21	theme	solar	1259:1263	arg1	evaporator					1265:1274	This solar evaporator	1254:1274	This solar evaporator	1254:1274	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	2	22	theme	composite	309:317	arg1	wood					330:333	composite carbonized wood	309:333	composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel	309:384	In this study, a solar evaporator was prepared by assembling composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel.
36149391	8	23	dep	mechanism	1605:1613	arg1	principles					1626:1635	principles	1626:1635	principles	1626:1635	Also, experiments and numerical simulation were carried out to understand the mechanism and design principles of this solar evaporators.
36149391	8	23	dep	mechanism	1605:1613	arg1	the					1601:1603	the	1601:1603	the	1601:1603	Also, experiments and numerical simulation were carried out to understand the mechanism and design principles of this solar evaporators.
36149391	3	24	theme	multilayer	391:400	arg1	Ti3C2T					409:414	Ti3C2T	409:414	Ti3C2T	409:414	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	3	24	theme	multilayer	391:400	arg1	embedded					421:428	embedded	421:428	embedded	421:428	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	3	24	theme	multilayer	391:400	arg1	MXene					402:406	The multilayer MXene	387:406	The multilayer MXene (Ti3C2T)	387:415	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	4	25	theme	high	719:722	arg1	energy					732:737	high binding energy	719:737	high binding energy with water molecules	719:758	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	5	26	theme	light	936:940	arg1	bands					942:946	the infrared and ultraviolet light bands	907:946	bands	942:946	Moreover, the addition of MXene can improve the light absorbance, especially for the infrared and ultraviolet light bands.
36149391	2	27	theme	hydrogel	377:384	arg1	wood					330:333	composite carbonized wood	309:333	composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel	309:384	In this study, a solar evaporator was prepared by assembling composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel.
36149391	0	28	theme	Solar	33:37	arg1	Evaporator					39:48	Highly Effective Multifunctional Solar Evaporator	0:48	Highly Effective Multifunctional Solar Evaporator with Scaffolding	0:65	Highly Effective Multifunctional Solar Evaporator with Scaffolding Structured Carbonized Wood and Biohydrogel.
36149391	7	29	theme	%	1358:1358	arg1	efficiency					1338:1347	an evaporation efficiency	1323:1347	an evaporation efficiency of 129.64%	1323:1358	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	7	29	theme	%	1358:1358	arg1	rate					1295:1298	an evaporation rate	1280:1298	an evaporation rate of 3.71 kg·m-2·h-1	1280:1317	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	3	30	theme	scaffolding	534:544	arg1	structure					546:554	the loose and ordered scaffolding structure	512:554	the loose and ordered scaffolding structure of the carbonized wood	512:577	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	6	31	theme	evaporator	1242:1251	arg1	surface					1227:1233	the surface	1223:1233	the surface of the evaporator	1223:1251	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	7	32	theme	kg·m-2·h-1	1308:1317	arg1	efficiency					1338:1347	an evaporation efficiency	1323:1347	an evaporation efficiency of 129.64%	1323:1358	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	7	32	theme	kg·m-2·h-1	1308:1317	arg1	rate					1295:1298	an evaporation rate	1280:1298	an evaporation rate of 3.71 kg·m-2·h-1	1280:1317	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	0	33	theme	Multifunctional	17:31	arg1	Evaporator					39:48	Highly Effective Multifunctional Solar Evaporator	0:48	Highly Effective Multifunctional Solar Evaporator with Scaffolding	0:65	Highly Effective Multifunctional Solar Evaporator with Scaffolding Structured Carbonized Wood and Biohydrogel.
36149391	7	34	theme	open-circuit	1417:1428	arg1	voltage					1430:1436	an open-circuit voltage	1414:1436	an open-circuit voltage of 1.8 mV	1414:1446	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	3	35	theme	increased	646:654	arg1	force					666:670	increased capillary force	646:670	increased capillary force	646:670	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	4	36	theme	water	744:748	arg1	molecules					750:758	water molecules	744:758	water molecules	744:758	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	1	37	theme	energy	208:213	arg1	crisis					215:220	energy crisis	208:220	energy crisis	208:220	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	8	38	theme	numerical	1549:1557	arg1	simulation					1559:1568	numerical simulation	1549:1568	numerical simulation	1549:1568	Also, experiments and numerical simulation were carried out to understand the mechanism and design principles of this solar evaporators.
36149391	3	39	theme	capillary	656:664	arg1	force					666:670	increased capillary force	646:670	increased capillary force	646:670	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	4	40	theme	angle	819:823	arg1	reduction					772:780	reduction	772:780	reduction of vaporization enthalpy and contact angle	772:823	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	7	41	theme	evaporation	1326:1336	arg1	efficiency					1338:1347	an evaporation efficiency	1323:1347	an evaporation efficiency of 129.64%	1323:1358	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	3	42	theme	scaffolding	437:447	arg1	structure					449:457	the scaffolding structure	433:457	the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force	433:670	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	5	43	theme	MXene	852:856	arg1	addition					840:847	the addition	836:847	the addition of MXene	836:856	Moreover, the addition of MXene can improve the light absorbance, especially for the infrared and ultraviolet light bands.
36149391	3	44	theme	transportation	626:639	arg1	efficiency					606:615	the efficiency	602:615	the efficiency of water transportation	602:639	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	4	45	theme	contact	811:817	arg1	angle					819:823	contact angle	811:823	contact angle	811:823	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	8	46	theme	evaporators	1651:1661	arg1	mechanism					1605:1613	mechanism	1605:1613	mechanism	1605:1613	Also, experiments and numerical simulation were carried out to understand the mechanism and design principles of this solar evaporators.
36149391	8	46	theme	evaporators	1651:1661	arg1	design					1619:1624	design	1619:1624	design	1619:1624	Also, experiments and numerical simulation were carried out to understand the mechanism and design principles of this solar evaporators.
36149391	5	47	theme	light	874:878	arg1	absorbance					880:889	the light absorbance	870:889	the light absorbance	870:889	Moreover, the addition of MXene can improve the light absorbance, especially for the infrared and ultraviolet light bands.
36149391	3	48	theme	carbonized	563:572	arg1	wood					574:577	the carbonized wood	559:577	the carbonized wood	559:577	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	3	49	from	structure	449:457	arg1	embedded					421:428	embedded	421:428	embedded	421:428	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	3	49	from	structure	449:457	arg1	MXene					402:406	The multilayer MXene	387:406	The multilayer MXene (Ti3C2T)	387:415	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	6	50	theme	evaporation	1127:1137	arg1	efficiency					1139:1148	the evaporation efficiency	1123:1148	the evaporation efficiency	1123:1148	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	7	51	theme	hydrovoltaic	1463:1474	arg1	min					1459:1461	a 20 min hydrovoltaic	1454:1474	a 20 min hydrovoltaic	1454:1474	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	1	52	with	deal	198:201	arg1	shortage					238:245	fresh water shortage	226:245	fresh water shortage	226:245	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	1	52	with	deal	198:201	arg1	crisis					215:220	energy crisis	208:220	energy crisis	208:220	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	3	53	theme	wood	574:577	arg1	structure					546:554	the loose and ordered scaffolding structure	512:554	the loose and ordered scaffolding structure of the carbonized wood	512:577	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	7	54	theme	evaporation	1283:1293	arg1	rate					1295:1298	an evaporation rate	1280:1298	an evaporation rate of 3.71 kg·m-2·h-1	1280:1317	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	3	55	theme	composite	479:487	arg1	wood					500:503	composite carbonized wood	479:503	composite carbonized wood	479:503	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	4	56	theme	binding	724:730	arg1	energy					732:737	high binding energy	719:737	high binding energy with water molecules	719:758	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	4	57	with	energy	732:737	arg1	molecules					750:758	water molecules	744:758	water molecules	744:758	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	4	58	theme	vaporization	785:796	arg1	enthalpy					798:805	vaporization enthalpy	785:805	vaporization enthalpy	785:805	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36149391	6	59	theme	alginate	1023:1030	arg1	polysaccharides					1032:1046	konjac glucomannan and sodium alginate polysaccharides	993:1046	polysaccharides	1032:1046	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	1	60	theme	solar	144:148	arg1	radiation					150:158	solar radiation	144:158	solar radiation energy	144:165	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	1	61	theme	solar	113:117	arg1	approach					186:193	a renewable approach	174:193	a renewable approach to deal with energy crisis and fresh water shortage	174:245	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	1	61	theme	solar	113:117	arg1	evaporator					119:128	A solar evaporator	111:128	A solar evaporator that utilizes solar radiation energy	111:165	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	1	62	theme	fresh	226:230	arg1	shortage					238:245	fresh water shortage	226:245	fresh water shortage	226:245	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	6	63	theme	temperature	1168:1178	arg1	distribution					1180:1191	the temperature distribution	1164:1191	the temperature distribution	1164:1191	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	3	64	theme	water	620:624	arg1	transportation					626:639	water transportation	620:639	water transportation	620:639	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	1	65	theme	radiation	150:158	arg1	energy					160:165	solar radiation energy	144:165	solar radiation energy	144:165	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	1	66	theme	water	232:236	arg1	shortage					238:245	fresh water shortage	226:245	fresh water shortage	226:245	A solar evaporator that utilizes solar radiation energy can be a renewable approach to deal with energy crisis and fresh water shortage.
36149391	7	67	theme	high	1493:1496	arg1	performance					1498:1508	a high performance	1491:1508	a high performance	1491:1508	This solar evaporator has an evaporation rate of 3.71 kg·m-2·h-1 and an evaporation efficiency of 129.64% under 2 sun illumination and is available to generate an open-circuit voltage of 1.8 mV after a 20 min hydrovoltaic, demonstrating a high performance and versatility.
36149391	6	68	with	glucomannan	1000:1010	arg1	Ca2+					1053:1056	Ca2+	1053:1056	Ca2+	1053:1056	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	3	69	from	embedded	421:428	arg1	structure					449:457	the scaffolding structure	433:457	the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force	433:670	The multilayer MXene (Ti3C2T) was embedded in the scaffolding structure of the wood to form composite carbonized wood, where the loose and ordered scaffolding structure of the carbonized wood significantly improves the efficiency of water transportation with increased capillary force.
36149391	2	70	theme	biobased	368:375	arg1	hydrogel					377:384	biobased hydrogel	368:384	biobased hydrogel	368:384	In this study, a solar evaporator was prepared by assembling composite carbonized wood of Melaleuca Leucadendron L. and biobased hydrogel.
36149391	8	71	theme	solar	1645:1649	arg1	evaporators					1651:1661	this solar evaporators	1640:1661	this solar evaporators	1640:1661	Also, experiments and numerical simulation were carried out to understand the mechanism and design principles of this solar evaporators.
36149391	6	72	theme	sodium	1016:1021	arg1	polysaccharides					1032:1046	konjac glucomannan and sodium alginate polysaccharides	993:1046	polysaccharides	1032:1046	The hydrogel was fabricated by crosslinking konjac glucomannan and sodium alginate polysaccharides with Ca2+, and it has a lower thermal conductivity than water and improves the evaporation efficiency by regulating the temperature distribution and concentrating the heat on the surface of the evaporator.
36149391	4	73	theme	enthalpy	798:805	arg1	reduction					772:780	reduction	772:780	reduction of vaporization enthalpy and contact angle	772:823	The MXene adsorbed in the carbonized wood has high binding energy with water molecules, leading to reduction of vaporization enthalpy and contact angle.
36185700	5	0	with	SLMPs-2-1	946:954	arg1	weights					981:987	different molecular weights	961:987	different molecular weights of 1.03 and 1.02 kDa	961:1008	The results showed that SLMPs-1-1 and SLMPs-2-1 with different molecular weights of 1.03 and 1.02 kDa, mainly composed of glucose (46.76 and 46.79%), respectively.
36185700	7	1	theme	factor-α	1358:1365	arg1	levels					1333:1338	the levels	1329:1338	the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2)	1329:1398	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	2	2	theme	major	310:314	arg1	compounds					326:334	the major bioactive compounds	306:334	the major bioactive compounds in the SLM	306:345	Polysaccharides have been identified as one of the major bioactive compounds in the SLM.
36185700	6	3	from	SEM	1139:1141	arg1	results					1076:1082	The results	1072:1082	The results of structural characterization from FT-IR, 1H NMR, and SEM	1072:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	3	from	SEM	1139:1141	arg1	characterization					1098:1113	structural characterization	1087:1113	structural characterization from FT-IR, 1H NMR, and SEM	1087:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	4	theme	β-glycosidic	1254:1265	arg1	bond					1267:1270	β-glycosidic bond	1254:1270	β-glycosidic bond	1254:1270	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	5	from	FT-IR	1120:1124	arg1	results					1076:1082	The results	1072:1082	The results of structural characterization from FT-IR, 1H NMR, and SEM	1072:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	5	from	FT-IR	1120:1124	arg1	characterization					1098:1113	structural characterization	1087:1113	structural characterization from FT-IR, 1H NMR, and SEM	1087:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	7	6	theme	cyclophosphamide	1451:1466	arg1	mice					1481:1484	cyclophosphamide (Cy)-treated mice	1451:1484	cyclophosphamide (Cy)-treated mice	1451:1484	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	0	7	theme	Maxim	96:100	arg1	polysaccharide					102:115	Sibiraea laexigata (L.) Maxim polysaccharide	72:115	Sibiraea laexigata (L.) Maxim polysaccharide	72:115	Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide.
36185700	3	8	theme	DEAE	625:628	arg1	Cellulose-52					630:641	DEAE Cellulose-52 and Sephadex G-100 chromatography	625:675	Cellulose-52	630:641	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	6	9	theme	1H	1127:1128	arg1	NMR					1130:1132	1H NMR	1127:1132	1H NMR	1127:1132	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	8	10	theme	mice	1697:1700	arg1	protection					1672:1681	immunosuppressive protection	1654:1681	immunosuppressive protection of Cy-treated mice	1654:1700	RT-qPCR and Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the levels of NF-κB, TLR4, which revealed that SLMPs-1-1 could participate in immunosuppressive protection of Cy-treated mice.
36185700	4	11	theme	spectra	882:888	arg1	analysis					890:897	nuclear magnetic resonance (NMR) spectra analysis	849:897	nuclear magnetic resonance (NMR) spectra analysis	849:897	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	8	12	dep	up-regulated	1563:1574	arg1	could					1543:1547	could	1543:1547	could	1543:1547	RT-qPCR and Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the levels of NF-κB, TLR4, which revealed that SLMPs-1-1 could participate in immunosuppressive protection of Cy-treated mice.
36185700	7	13	theme	necrosis	1349:1356	arg1	TNF-α					1368:1372	TNF-α	1368:1372	TNF-α	1368:1372	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	7	13	theme	necrosis	1349:1356	arg1	factor-α					1358:1365	tumor necrosis factor-α	1343:1365	tumor necrosis factor-α (TNF-α)	1343:1373	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	7	14	theme	tumor	1343:1347	arg1	TNF-α					1368:1372	TNF-α	1368:1372	TNF-α	1368:1372	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	7	14	theme	tumor	1343:1347	arg1	factor-α					1358:1365	tumor necrosis factor-α	1343:1365	tumor necrosis factor-α (TNF-α)	1343:1373	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	9	15	theme	food	1812:1815	arg1	industries					1836:1845	food and pharmaceutical industries	1812:1845	food and pharmaceutical industries	1812:1845	These findings suggested that the potential of SLMPs-1-1 as an alternative immunostimulator could be used in food and pharmaceutical industries.
36185700	6	16	from	results	1076:1082	arg1	FT-IR					1120:1124	FT-IR	1120:1124	FT-IR	1120:1124	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	16	from	results	1076:1082	arg1	NMR					1130:1132	1H NMR	1127:1132	1H NMR	1127:1132	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	16	from	results	1076:1082	arg1	SEM					1139:1141	SEM	1139:1141	SEM	1139:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	8	17	theme	NF-κB	1590:1594	arg1	levels					1580:1585	the levels	1576:1585	the levels	1576:1585	RT-qPCR and Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the levels of NF-κB, TLR4, which revealed that SLMPs-1-1 could participate in immunosuppressive protection of Cy-treated mice.
36185700	3	18	theme	enzymatic	390:398	arg1	method					418:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	0	19	theme	polysaccharide	102:115	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide.
36185700	0	19	theme	polysaccharide	102:115	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide.
36185700	0	19	theme	polysaccharide	102:115	arg1	activity					60:67	immunological activity	46:67	immunological activity	46:67	Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide.
36185700	3	20	theme	Sephadex	647:654	arg1	chromatography					662:675	DEAE Cellulose-52 and Sephadex G-100 chromatography	625:675	chromatography	662:675	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	21	theme	present	355:361	arg1	paper					363:367	the present paper	351:367	the present paper	351:367	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	1	22	dep	laexigata	127:135	arg1	L.					138:139	L.	138:139	L.	138:139	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	9	23	theme	pharmaceutical	1821:1834	arg1	industries					1836:1845	food and pharmaceutical industries	1812:1845	food and pharmaceutical industries	1812:1845	These findings suggested that the potential of SLMPs-1-1 as an alternative immunostimulator could be used in food and pharmaceutical industries.
36185700	3	24	theme	extraction	400:409	arg1	method					418:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	25	theme	G-100	656:660	arg1	chromatography					662:675	DEAE Cellulose-52 and Sephadex G-100 chromatography	625:675	chromatography	662:675	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	4	26	theme	weight	807:812	arg1	measurement					814:824	molecular weight measurement	797:824	molecular weight measurement	797:824	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	6	27	contain	contained	1181:1189	arg1	SLMPs-2-1					1171:1179	SLMPs-2-1	1171:1179	SLMPs-2-1	1171:1179	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	27	contain	contained	1181:1189	arg2	polysaccharide					1212:1225	the typical pyranoid polysaccharide	1191:1225	the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond	1191:1270	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	27	contain	contained	1181:1189	arg1	SLMPs-1-1					1157:1165	SLMPs-1-1	1157:1165	SLMPs-1-1	1157:1165	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	1	28	theme	long	239:242	arg1	time					244:247	a long time	237:247	a long time in china	237:256	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	1	29	from	time	244:247	arg1	china					252:256	china	252:256	china	252:256	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	2	30	from	compounds	326:334	arg1	SLM					343:345	the SLM	339:345	the SLM	339:345	Polysaccharides have been identified as one of the major bioactive compounds in the SLM.
36185700	3	31	theme	extraction	520:529	arg1	solvent					531:537	extraction solvent	520:537	extraction solvent	520:537	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	32	theme	ultrasonic-assisted	370:388	arg1	method					418:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	33	from	chromatography	662:675	arg1	sequence					680:687	sequence	680:687	sequence	680:687	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	6	34	theme	characterization	1098:1113	arg1	results					1076:1082	The results	1072:1082	The results of structural characterization from FT-IR, 1H NMR, and SEM	1072:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	4	35	theme	magnetic	857:864	arg1	NMR					877:879	NMR	877:879	NMR	877:879	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	4	35	theme	magnetic	857:864	arg1	resonance					866:874	nuclear magnetic resonance	849:874	nuclear magnetic resonance (NMR) spectra analysis	849:897	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	5	36	theme	different	961:969	arg1	weights					981:987	different molecular weights	961:987	different molecular weights of 1.03 and 1.02 kDa	961:1008	The results showed that SLMPs-1-1 and SLMPs-2-1 with different molecular weights of 1.03 and 1.02 kDa, mainly composed of glucose (46.76 and 46.79%), respectively.
36185700	9	37	theme	SLMPs-1-1	1750:1758	arg1	potential					1737:1745	the potential	1733:1745	the potential of SLMPs-1-1 as an alternative immunostimulator	1733:1793	These findings suggested that the potential of SLMPs-1-1 as an alternative immunostimulator could be used in food and pharmaceutical industries.
36185700	0	38	theme	structural	14:23	arg1	characterization					25:40	structural characterization	14:40	structural characterization	14:40	Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide.
36185700	4	39	theme	nuclear	849:855	arg1	NMR					877:879	NMR	877:879	NMR	877:879	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	4	39	theme	nuclear	849:855	arg1	resonance					866:874	nuclear magnetic resonance	849:874	nuclear magnetic resonance (NMR) spectra analysis	849:897	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	7	40	theme	immune	1420:1425	arg1	organs					1427:1432	immune organs	1420:1432	the immune organs tissue damage of cyclophosphamide (Cy)-treated mice	1416:1484	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	6	41	with	polysaccharide	1212:1225	arg1	bond					1245:1248	α-glycosidic bond	1232:1248	α-glycosidic bond	1232:1248	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	41	with	polysaccharide	1212:1225	arg1	bond					1267:1270	β-glycosidic bond	1254:1270	β-glycosidic bond	1254:1270	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	0	42	theme	immunological	46:58	arg1	activity					60:67	immunological activity	46:67	immunological activity	46:67	Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide.
36185700	8	43	theme	Western-Blot	1499:1510	arg1	analysis					1512:1519	RT-qPCR and Western-Blot analysis	1487:1519	analysis	1512:1519	RT-qPCR and Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the levels of NF-κB, TLR4, which revealed that SLMPs-1-1 could participate in immunosuppressive protection of Cy-treated mice.
36185700	6	44	theme	α-glycosidic	1232:1243	arg1	bond					1245:1248	α-glycosidic bond	1232:1248	α-glycosidic bond	1232:1248	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	7	45	theme	organs	1427:1432	arg1	damage					1441:1446	the immune organs tissue damage	1416:1446	the immune organs tissue damage of cyclophosphamide (Cy)-treated mice	1416:1484	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	1	46	theme	herbal	174:179	arg1	laexigata					127:135	Sibiraea laexigata	118:135	Sibiraea laexigata (L.) Maxim (SLM)	118:152	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	1	46	theme	herbal	174:179	arg1	tea					181:183	an herbal tea	171:183	an herbal tea for treating stomach discomfort and indigestion for a long time in china	171:256	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	4	47	theme	resonance	866:874	arg1	analysis					890:897	nuclear magnetic resonance (NMR) spectra analysis	849:897	nuclear magnetic resonance (NMR) spectra analysis	849:897	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	5	48	theme	kDa	1006:1008	arg1	weights					981:987	different molecular weights	961:987	different molecular weights of 1.03 and 1.02 kDa	961:1008	The results showed that SLMPs-1-1 and SLMPs-2-1 with different molecular weights of 1.03 and 1.02 kDa, mainly composed of glucose (46.76 and 46.79%), respectively.
36185700	3	49	theme	SLM	544:546	arg1	SLMPs-1-1					583:591	SLMPs-1-1	583:591	SLMPs-1-1	583:591	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	49	theme	SLM	544:546	arg1	fractions					572:580	two SLM polysaccharides (SLMPs) fractions	540:580	two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1)	540:606	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	49	theme	SLM	544:546	arg1	SLMPs-2-1					597:605	SLMPs-2-1	597:605	SLMPs-2-1	597:605	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	50	theme	UAEE	412:415	arg1	method					418:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	ultrasonic-assisted enzymatic extraction (UAEE) method	370:423	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	51	from	Cellulose-52	630:641	arg1	sequence					680:687	sequence	680:687	sequence	680:687	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	4	52	theme	composition	775:785	arg1	analysis					787:794	chemical composition analysis	766:794	chemical composition analysis	766:794	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	4	53	theme	molecular	797:805	arg1	measurement					814:824	molecular weight measurement	797:824	molecular weight measurement	797:824	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	7	54	theme	tissue	1434:1439	arg1	damage					1441:1446	the immune organs tissue damage	1416:1446	the immune organs tissue damage of cyclophosphamide (Cy)-treated mice	1416:1484	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	9	55	used	used	1804:1807	arg2	potential					1737:1745	the potential	1733:1745	the potential of SLMPs-1-1 as an alternative immunostimulator	1733:1793	These findings suggested that the potential of SLMPs-1-1 as an alternative immunostimulator could be used in food and pharmaceutical industries.
36185700	7	56	theme	mice	1481:1484	arg1	damage					1441:1446	the immune organs tissue damage	1416:1446	the immune organs tissue damage of cyclophosphamide (Cy)-treated mice	1416:1484	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	6	57	theme	pyranoid	1203:1210	arg1	polysaccharide					1212:1225	the typical pyranoid polysaccharide	1191:1225	the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond	1191:1270	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	5	58	theme	molecular	971:979	arg1	weights					981:987	different molecular weights	961:987	different molecular weights of 1.03 and 1.02 kDa	961:1008	The results showed that SLMPs-1-1 and SLMPs-2-1 with different molecular weights of 1.03 and 1.02 kDa, mainly composed of glucose (46.76 and 46.79%), respectively.
36185700	4	59	theme	chemical	766:773	arg1	analysis					787:794	chemical composition analysis	766:794	chemical composition analysis	766:794	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	3	60	attach	derived	468:474	arg2	extraction					457:466	polysaccharides extraction	441:466	polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent	441:537	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	60	attach	derived	468:474	arg1	SLM					481:483	SLM	481:483	SLM	481:483	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	61	theme	polysaccharides	441:455	arg1	extraction					457:466	polysaccharides extraction	441:466	polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent	441:537	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	6	62	theme	structural	1087:1096	arg1	characterization					1098:1113	structural characterization	1087:1113	structural characterization from FT-IR, 1H NMR, and SEM	1087:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	63	theme	typical	1195:1201	arg1	polysaccharide					1212:1225	the typical pyranoid polysaccharide	1191:1225	the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond	1191:1270	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	3	64	theme	SLMPs	565:569	arg1	SLMPs-1-1					583:591	SLMPs-1-1	583:591	SLMPs-1-1	583:591	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	64	theme	SLMPs	565:569	arg1	fractions					572:580	two SLM polysaccharides (SLMPs) fractions	540:580	two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1)	540:606	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	64	theme	SLMPs	565:569	arg1	SLMPs-2-1					597:605	SLMPs-2-1	597:605	SLMPs-2-1	597:605	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	1	65	used	used	163:166	arg2	tea					181:183	an herbal tea	171:183	an herbal tea for treating stomach discomfort and indigestion for a long time in china	171:256	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	1	65	used	used	163:166	arg2	SLM					149:151	SLM	149:151	SLM	149:151	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	1	65	used	used	163:166	arg2	Maxim					142:146	Maxim	142:146	Maxim	142:146	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	1	65	used	used	163:166	arg2	laexigata					127:135	Sibiraea laexigata	118:135	Sibiraea laexigata (L.) Maxim (SLM)	118:152	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	8	66	theme	immunosuppressive	1654:1670	arg1	protection					1672:1681	immunosuppressive protection	1654:1681	immunosuppressive protection of Cy-treated mice	1654:1700	RT-qPCR and Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the levels of NF-κB, TLR4, which revealed that SLMPs-1-1 could participate in immunosuppressive protection of Cy-treated mice.
36185700	3	67	dep	fractions	572:580	arg1	SLMPs-1-1					583:591	SLMPs-1-1	583:591	SLMPs-1-1	583:591	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	67	dep	fractions	572:580	arg1	fractions					572:580	two SLM polysaccharides (SLMPs) fractions	540:580	two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1)	540:606	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	67	dep	fractions	572:580	arg1	SLMPs-2-1					597:605	SLMPs-2-1	597:605	SLMPs-2-1	597:605	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	68	theme	polyethylene	491:502	arg1	glycol					504:509	polyethylene glycol	491:509	polyethylene glycol (PEG)	491:515	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	68	theme	polyethylene	491:502	arg1	PEG					512:514	PEG	512:514	PEG	512:514	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	8	69	theme	Cy-treated	1686:1695	arg1	mice					1697:1700	Cy-treated mice	1686:1700	Cy-treated mice	1686:1700	RT-qPCR and Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the levels of NF-κB, TLR4, which revealed that SLMPs-1-1 could participate in immunosuppressive protection of Cy-treated mice.
36185700	9	70	theme	alternative	1766:1776	arg1	immunostimulator					1778:1793	an alternative immunostimulator	1763:1793	an alternative immunostimulator	1763:1793	These findings suggested that the potential of SLMPs-1-1 as an alternative immunostimulator could be used in food and pharmaceutical industries.
36185700	7	71	theme	-treated	1472:1479	arg1	mice					1481:1484	cyclophosphamide (Cy)-treated mice	1451:1484	cyclophosphamide (Cy)-treated mice	1451:1484	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	0	72	theme	laexigata	81:89	arg1	polysaccharide					102:115	Sibiraea laexigata (L.) Maxim polysaccharide	72:115	Sibiraea laexigata (L.) Maxim polysaccharide	72:115	Purification, structural characterization and immunological activity of Sibiraea laexigata (L.) Maxim polysaccharide.
36185700	3	73	theme	polysaccharides	548:562	arg1	SLMPs-1-1					583:591	SLMPs-1-1	583:591	SLMPs-1-1	583:591	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	73	theme	polysaccharides	548:562	arg1	fractions					572:580	two SLM polysaccharides (SLMPs) fractions	540:580	two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1)	540:606	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	3	73	theme	polysaccharides	548:562	arg1	SLMPs-2-1					597:605	SLMPs-2-1	597:605	SLMPs-2-1	597:605	In the present paper, ultrasonic-assisted enzymatic extraction (UAEE) method was employed in polysaccharides extraction derived from SLM using polyethylene glycol (PEG) as extraction solvent, two SLM polysaccharides (SLMPs) fractions (SLMPs-1-1 and SLMPs-2-1) were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence.
36185700	2	74	theme	compounds	326:334	arg1	one					299:301	one	299:301	one	299:301	Polysaccharides have been identified as one of the major bioactive compounds in the SLM.
36185700	2	74	theme	compounds	326:334	arg1	Polysaccharides					259:273	Polysaccharides	259:273	Polysaccharides	259:273	Polysaccharides have been identified as one of the major bioactive compounds in the SLM.
36185700	2	74	theme	compounds	326:334	arg1	compounds					326:334	the major bioactive compounds	306:334	the major bioactive compounds in the SLM	306:345	Polysaccharides have been identified as one of the major bioactive compounds in the SLM.
36185700	6	75	from	NMR	1130:1132	arg1	results					1076:1082	The results	1072:1082	The results of structural characterization from FT-IR, 1H NMR, and SEM	1072:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	6	75	from	NMR	1130:1132	arg1	characterization					1098:1113	structural characterization	1087:1113	structural characterization from FT-IR, 1H NMR, and SEM	1087:1141	The results of structural characterization from FT-IR, 1H NMR, and SEM revealed that SLMPs-1-1 and SLMPs-2-1 contained the typical pyranoid polysaccharide with α-glycosidic bond and β-glycosidic bond.
36185700	1	76	theme	stomach	198:204	arg1	discomfort					206:215	stomach discomfort	198:215	stomach discomfort	198:215	Sibiraea laexigata (L.) Maxim (SLM) has been used as an herbal tea for treating stomach discomfort and indigestion for a long time in china.
36185700	5	77	with	SLMPs-1-1	932:940	arg1	weights					981:987	different molecular weights	961:987	different molecular weights of 1.03 and 1.02 kDa	961:1008	The results showed that SLMPs-1-1 and SLMPs-2-1 with different molecular weights of 1.03 and 1.02 kDa, mainly composed of glucose (46.76 and 46.79%), respectively.
36185700	7	78	theme	interleukin-2	1379:1391	arg1	levels					1333:1338	the levels	1329:1338	the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2)	1329:1398	Furthermore, it was found that SLMPs-1-1 could increase the levels of tumor necrosis factor-α (TNF-α) and interleukin-2 (IL-2), and alleviated the immune organs tissue damage of cyclophosphamide (Cy)-treated mice.
36185700	4	79	theme	factions	735:742	arg1	structure					714:722	the preliminarily structure	696:722	the preliminarily structure of the two factions	696:742	Then, the preliminarily structure of the two factions were characterized by chemical composition analysis, molecular weight measurement, UVS, HPLC-PMP, FT-IR, nuclear magnetic resonance (NMR) spectra analysis and SEM.
36185700	2	80	theme	bioactive	316:324	arg1	compounds					326:334	the major bioactive compounds	306:334	the major bioactive compounds in the SLM	306:345	Polysaccharides have been identified as one of the major bioactive compounds in the SLM.
36185700	8	81	theme	RT-qPCR	1487:1493	arg1	analysis					1512:1519	RT-qPCR and Western-Blot analysis	1487:1519	analysis	1512:1519	RT-qPCR and Western-Blot analysis showed that SLMPs-1-1 could significantly up-regulated the levels of NF-κB, TLR4, which revealed that SLMPs-1-1 could participate in immunosuppressive protection of Cy-treated mice.
35684352	8	0	theme	anticancer	1819:1828	arg1	effects					1830:1836	antioxidant and anticancer effects	1803:1836	antioxidant and anticancer effects	1803:1836	Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
35684352	4	1	theme	major	838:842	arg1	acid					806:809	Glutamic acid	797:809	Glutamic acid	797:809	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	1	theme	major	838:842	arg1	acid					824:827	aspartic acid	815:827	aspartic acid	815:827	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	1	theme	major	838:842	arg1	AAs					844:846	the major AAs	834:846	the major AAs in all halophyte species	834:871	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	1	2	theme	amino	221:225	arg1	AAs					234:236	AAs	234:236	AAs	234:236	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	2	theme	amino	221:225	arg1	acids					227:231	amino acids	221:231	amino acids (AAs)	221:237	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	2	3	from	rich	491:494	arg1	naringenin					499:508	naringenin	499:508	naringenin with 11.88 mg/g	499:524	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	2	4	with	naringenin	499:508	arg1	mg/g					521:524	11.88 mg/g	515:524	11.88 mg/g	515:524	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	6	5	theme	unsaponifiable	1241:1254	arg1	constituents					1269:1280	the major unsaponifiable matter (USM) constituents	1231:1280	the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii	1231:1313	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	1	6	theme	carbohydrates	163:175	arg1	compositions					147:158	the compositions	143:158	the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	143:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	4	7	theme	total	903:907	arg1	AAs					909:911	the total AAs	899:911	the total AAs	899:911	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	1	8	theme	phenolic	178:185	arg1	compounds					187:195	phenolic compounds	178:195	phenolic compounds	178:195	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	8	9	with	compounds	1788:1796	arg1	effects					1830:1836	antioxidant and anticancer effects	1803:1836	antioxidant and anticancer effects	1803:1836	Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
35684352	3	10	from	glucose	743:749	arg1	aegyptiaca					652:661	S. aegyptiaca	649:661	S. aegyptiaca	649:661	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	10	from	glucose	743:749	arg1	album					790:794	Z. album	787:794	Z. album	787:794	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	10	from	glucose	743:749	arg1	monoica					716:722	S. monoica	713:722	S. monoica	713:722	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	10	from	glucose	743:749	arg1	ehrenbergii					616:626	A. ehrenbergii	613:626	A. ehrenbergii	613:626	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	4	11	theme	10-22	889:893	arg1	%					894:894	10-22%	889:894	10-22%	889:894	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	11	theme	10-22	889:893	arg1	AAs					909:911	the total AAs	899:911	the total AAs	899:911	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	1	12	theme	Zygophyllum	328:338	arg1	album					340:344	Zygophyllum album	328:344	Zygophyllum album	328:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	12	theme	Zygophyllum	328:338	arg1	halophytes					255:264	four Rea Sea halophytes	242:264	four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	242:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	13	theme	acids	204:208	arg1	compositions					147:158	the compositions	143:158	the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	143:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	7	14	theme	IC50	1643:1646	arg1	values					1648:1653	IC50 values	1643:1653	IC50 values	1643:1653	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	2	15	from	acid	420:423	arg1	rich					405:408	rich	405:408	rich	405:408	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	6	16	theme	monoica	1288:1294	arg1	constituents					1269:1280	the major unsaponifiable matter (USM) constituents	1231:1280	the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii	1231:1313	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	3	17	dep	glucose	743:749	arg1	%					767:767	36.67 and 31.52%	752:767	36.67 and 31.52%	752:767	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	7	18	theme	noteworthy	1431:1440	arg1	activity					1454:1461	noteworthy antioxidant activity	1431:1461	noteworthy antioxidant activity	1431:1461	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	3	19	from	arabinose	729:737	arg1	aegyptiaca					652:661	S. aegyptiaca	649:661	S. aegyptiaca	649:661	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	19	from	arabinose	729:737	arg1	monoica					716:722	S. monoica	713:722	S. monoica	713:722	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	19	from	arabinose	729:737	arg1	album					790:794	Z. album	787:794	Z. album	787:794	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	19	from	arabinose	729:737	arg1	ehrenbergii					616:626	A. ehrenbergii	613:626	A. ehrenbergii	613:626	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	5	20	theme	linolenic	1034:1042	arg1	acid					1044:1047	linolenic acid	1034:1047	linolenic acid (15.54-28.63%)	1034:1062	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	20	theme	linolenic	1034:1042	arg1	%					1061:1061	15.54-28.63%	1050:1061	15.54-28.63%	1050:1061	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	2	21	with	rich	405:408	arg1	mg/g					446:449	41.72 and 47.48 mg/g	430:449	41.72 and 47.48 mg/g	430:449	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	7	22	theme	monoica	1410:1416	arg1	fraction					1377:1384	The phenolic fraction	1364:1384	The phenolic fraction of S. aegyptiaca and S. monoica	1364:1416	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	2	23	theme	gallic	413:418	arg1	acid					420:423	gallic acid	413:423	gallic acid	413:423	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	7	24	theme	cell	1627:1630	arg1	lines					1632:1636	Caco-2 cancer cell lines	1613:1636	Caco-2 cancer cell lines	1613:1636	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	5	25	theme	saturated	1151:1159	arg1	acid					1167:1170	the most abundant saturated fatty acid	1133:1170	the most abundant saturated fatty acid (SFA)	1133:1176	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	25	theme	saturated	1151:1159	arg1	SFA					1173:1175	SFA	1173:1175	SFA	1173:1175	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	25	theme	saturated	1151:1159	arg1	acid					1109:1112	palmitic acid	1100:1112	palmitic acid (23.94-49.49%)	1100:1127	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	7	26	theme	Caco-2	1613:1618	arg1	lines					1632:1636	Caco-2 cancer cell lines	1613:1636	Caco-2 cancer cell lines	1613:1636	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	0	27	theme	Phytochemical	0:12	arg1	Compositions					14:25	Phytochemical Compositions	0:25	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.	0:102	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	1	28	dep	Suaeda	289:294	arg1	aegyptiaca					296:305	Suaeda aegyptiaca	289:305	Suaeda aegyptiaca	289:305	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	3	29	from	ribose	676:681	arg1	aegyptiaca					652:661	S. aegyptiaca	649:661	S. aegyptiaca	649:661	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	29	from	ribose	676:681	arg1	monoica					716:722	S. monoica	713:722	S. monoica	713:722	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	29	from	ribose	676:681	arg1	album					790:794	Z. album	787:794	Z. album	787:794	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	29	from	ribose	676:681	arg1	ehrenbergii					616:626	A. ehrenbergii	613:626	A. ehrenbergii	613:626	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	5	30	theme	unsaturated	946:956	arg1	UFA					971:973	UFA	971:973	UFA	971:973	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	30	theme	unsaturated	946:956	arg1	acids					964:968	unsaturated fatty acids	946:968	unsaturated fatty acids (UFA) of the four species	946:994	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	4	31	theme	Glutamic	797:804	arg1	acid					806:809	Glutamic acid	797:809	Glutamic acid	797:809	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	31	theme	Glutamic	797:804	arg1	acid					824:827	aspartic acid	815:827	aspartic acid	815:827	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	31	theme	Glutamic	797:804	arg1	AAs					844:846	the major AAs	834:846	the major AAs in all halophyte species	834:871	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	3	32	theme	species	559:565	arg1	polysaccharides					531:545	The polysaccharides	527:545	The polysaccharides of the four species	527:565	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	0	33	theme	Red	35:37	arg1	Plants					53:58	Some Red Sea Halophyte Plants	30:58	Some Red Sea Halophyte Plants	30:58	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	1	34	theme	Rea	247:249	arg1	album					340:344	Zygophyllum album	328:344	Zygophyllum album	328:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	34	theme	Rea	247:249	arg1	Suaeda					308:313	Suaeda	308:313	Suaeda	308:313	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	34	theme	Rea	247:249	arg1	Anabasis					267:274	Anabasis	267:274	Anabasis	267:274	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	34	theme	Rea	247:249	arg1	Suaeda					289:294	Suaeda	289:294	Suaeda	289:294	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	34	theme	Rea	247:249	arg1	halophytes					255:264	four Rea Sea halophytes	242:264	four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	242:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	0	35	theme	Halophyte	43:51	arg1	Plants					53:58	Some Red Sea Halophyte Plants	30:58	Some Red Sea Halophyte Plants	30:58	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	1	36	theme	halophytes	255:264	arg1	compositions					147:158	the compositions	143:158	the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	143:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	7	37	theme	cytotoxic	1568:1576	arg1	activity					1578:1585	potent cytotoxic activity	1561:1585	potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines	1561:1636	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	7	38	theme	FAs	1526:1528	arg1	fraction					1530:1537	the FAs fraction	1522:1537	the FAs fraction of Z. album	1522:1549	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	3	39	from	glucose	664:670	arg1	aegyptiaca					652:661	S. aegyptiaca	649:661	S. aegyptiaca	649:661	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	39	from	glucose	664:670	arg1	monoica					716:722	S. monoica	713:722	S. monoica	713:722	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	39	from	glucose	664:670	arg1	album					790:794	Z. album	787:794	Z. album	787:794	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	39	from	glucose	664:670	arg1	ehrenbergii					616:626	A. ehrenbergii	613:626	A. ehrenbergii	613:626	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	40	theme	Z.	787:788	arg1	album					790:794	Z. album	787:794	Z. album	787:794	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	8	41	theme	occurring	1778:1786	arg1	compounds					1788:1796	naturally occurring compounds	1768:1796	naturally occurring compounds with antioxidant and anticancer effects	1768:1836	Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
35684352	4	42	theme	50-63	878:882	arg1	%					883:883	50-63%	878:883	50-63%	878:883	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	42	theme	50-63	878:882	arg1	AAs					909:911	the total AAs	899:911	the total AAs	899:911	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	2	43	dep	A.	472:473	arg1	ehrenbergii					475:485	A. ehrenbergii	472:485	A. ehrenbergii	472:485	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	5	44	theme	acids	964:968	arg1	%					1011:1011	42.18-55.33%	1000:1011	42.18-55.33%	1000:1011	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	44	theme	acids	964:968	arg1	proportion					932:941	The proportion	928:941	The proportion of unsaturated fatty acids (UFA) of the four species	928:994	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	7	45	theme	μg/mL	1495:1499	arg1	values					1473:1478	IC50 values	1468:1478	IC50 values	1468:1478	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	1	46	theme	acids	227:231	arg1	compositions					147:158	the compositions	143:158	the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	143:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	5	47	theme	abundant	1142:1149	arg1	acid					1167:1170	the most abundant saturated fatty acid	1133:1170	the most abundant saturated fatty acid (SFA)	1133:1176	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	47	theme	abundant	1142:1149	arg1	SFA					1173:1175	SFA	1173:1175	SFA	1173:1175	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	47	theme	abundant	1142:1149	arg1	acid					1109:1112	palmitic acid	1100:1112	palmitic acid (23.94-49.49%)	1100:1127	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	6	48	theme	matter	1256:1261	arg1	constituents					1269:1280	the major unsaponifiable matter (USM) constituents	1231:1280	the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii	1231:1313	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	7	49	theme	μg/mL	1678:1682	arg1	values					1648:1653	IC50 values	1643:1653	IC50 values	1643:1653	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	4	50	theme	AAs	909:911	arg1	%					883:883	50-63%	878:883	50-63%	878:883	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	50	theme	AAs	909:911	arg1	%					894:894	10-22%	889:894	10-22%	889:894	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	50	theme	AAs	909:911	arg1	AAs					909:911	the total AAs	899:911	the total AAs	899:911	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	6	51	theme	major	1235:1239	arg1	constituents					1269:1280	the major unsaponifiable matter (USM) constituents	1231:1280	the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii	1231:1313	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	7	52	theme	IC50	1468:1471	arg1	values					1473:1478	IC50 values	1468:1478	IC50 values	1468:1478	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	1	53	theme	compounds	187:195	arg1	compositions					147:158	the compositions	143:158	the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	143:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	0	54	with	Compositions	14:25	arg1	Potentials					92:101	Antioxidant and Anticancer Potentials	65:101	Antioxidant and Anticancer Potentials	65:101	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	8	55	theme	future	1865:1870	arg1	applications					1872:1883	future applications	1865:1883	future applications	1865:1883	Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
35684352	3	56	from	galactose	591:599	arg1	aegyptiaca					652:661	S. aegyptiaca	649:661	S. aegyptiaca	649:661	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	56	from	galactose	591:599	arg1	ehrenbergii					616:626	A. ehrenbergii	613:626	A. ehrenbergii	613:626	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	56	from	galactose	591:599	arg1	album					790:794	Z. album	787:794	Z. album	787:794	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	56	from	galactose	591:599	arg1	monoica					716:722	S. monoica	713:722	S. monoica	713:722	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	1	57	theme	fatty	198:202	arg1	FAs					211:213	FAs	211:213	FAs	211:213	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	57	theme	fatty	198:202	arg1	acids					204:208	fatty acids	198:208	fatty acids (FAs)	198:214	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	58	theme	study	120:124	arg1	aim					108:110	The aim	104:110	The aim of this study	104:124	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	0	59	theme	Antioxidant	65:75	arg1	Potentials					92:101	Antioxidant and Anticancer Potentials	65:101	Antioxidant and Anticancer Potentials	65:101	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	7	60	theme	antioxidant	1442:1452	arg1	activity					1454:1461	noteworthy antioxidant activity	1431:1461	noteworthy antioxidant activity	1431:1461	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	0	61	theme	Anticancer	81:90	arg1	Potentials					92:101	Antioxidant and Anticancer Potentials	65:101	Antioxidant and Anticancer Potentials	65:101	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	6	62	theme	USM	1264:1266	arg1	constituents					1269:1280	the major unsaponifiable matter (USM) constituents	1231:1280	the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii	1231:1313	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	4	63	from	AAs	844:846	arg1	species					865:871	all halophyte species	851:871	all halophyte species	851:871	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	7	64	theme	cancer	1620:1625	arg1	lines					1632:1636	Caco-2 cancer cell lines	1613:1636	Caco-2 cancer cell lines	1613:1636	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	1	65	dep	halophytes	255:264	arg1	album					340:344	Zygophyllum album	328:344	Zygophyllum album	328:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	65	dep	halophytes	255:264	arg1	halophytes					255:264	four Rea Sea halophytes	242:264	four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	242:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	65	dep	halophytes	255:264	arg1	Anabasis					267:274	Anabasis	267:274	Anabasis	267:274	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	65	dep	halophytes	255:264	arg1	Suaeda					289:294	Suaeda	289:294	Suaeda	289:294	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	65	dep	halophytes	255:264	arg1	Suaeda					308:313	Suaeda	308:313	Suaeda	308:313	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	7	66	theme	aegyptiaca	1392:1401	arg1	fraction					1377:1384	The phenolic fraction	1364:1384	The phenolic fraction of S. aegyptiaca and S. monoica	1364:1416	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	2	67	from	rich	405:408	arg1	acid					420:423	gallic acid	413:423	gallic acid	413:423	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	5	68	theme	fatty	1161:1165	arg1	acid					1167:1170	the most abundant saturated fatty acid	1133:1170	the most abundant saturated fatty acid (SFA)	1133:1176	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	68	theme	fatty	1161:1165	arg1	SFA					1173:1175	SFA	1173:1175	SFA	1173:1175	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	68	theme	fatty	1161:1165	arg1	acid					1109:1112	palmitic acid	1100:1112	palmitic acid (23.94-49.49%)	1100:1127	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	69	theme	oleic	1068:1072	arg1	%					1090:1090	5.68-22.05%	1080:1090	5.68-22.05%	1080:1090	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	69	theme	oleic	1068:1072	arg1	acid					1074:1077	oleic acid	1068:1077	oleic acid (5.68-22.05%)	1068:1091	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	4	70	with	AAs	844:846	arg1	%					883:883	50-63%	878:883	50-63%	878:883	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	70	with	AAs	844:846	arg1	%					894:894	10-22%	889:894	10-22%	889:894	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	70	with	AAs	844:846	arg1	AAs					909:911	the total AAs	899:911	the total AAs	899:911	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	6	71	dep	proportions	1320:1330	arg1	42.44					1332:1336	42.44	1332:1336	42.44	1332:1336	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	6	71	dep	proportions	1320:1330	arg1	%					1347:1347	44.11%	1342:1347	44.11%	1342:1347	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	4	72	theme	aspartic	815:822	arg1	acid					806:809	Glutamic acid	797:809	Glutamic acid	797:809	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	72	theme	aspartic	815:822	arg1	acid					824:827	aspartic acid	815:827	aspartic acid	815:827	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	4	72	theme	aspartic	815:822	arg1	AAs					844:846	the major AAs	834:846	the major AAs in all halophyte species	834:871	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	2	73	from	naringenin	499:508	arg1	rich					491:494	rich	491:494	rich	491:494	The results showed that S. aegyptiaca and S. monoica were rich in gallic acid with 41.72 and 47.48 mg/g, respectively, while A. ehrenbergii was rich in naringenin with 11.88 mg/g.
35684352	6	74	theme	ehrenbergii	1303:1313	arg1	constituents					1269:1280	the major unsaponifiable matter (USM) constituents	1231:1280	the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii	1231:1313	Phytol and 9,19-cyclolanost-24-en-3β-ol represented the major unsaponifiable matter (USM) constituents of S. monoica and A. ehrenbergii with proportions 42.44 and 44.11%, respectively.
35684352	7	75	theme	phenolic	1368:1375	arg1	fraction					1377:1384	The phenolic fraction	1364:1384	The phenolic fraction of S. aegyptiaca and S. monoica	1364:1416	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	1	76	dep	Anabasis	267:274	arg1	ehrenbergii					276:286	Anabasis ehrenbergii	267:286	Anabasis ehrenbergii	267:286	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	0	77	theme	Sea	39:41	arg1	Plants					53:58	Some Red Sea Halophyte Plants	30:58	Some Red Sea Halophyte Plants	30:58	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	5	78	theme	fatty	958:962	arg1	UFA					971:973	UFA	971:973	UFA	971:973	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	78	theme	fatty	958:962	arg1	acids					964:968	unsaturated fatty acids	946:968	unsaturated fatty acids (UFA) of the four species	946:994	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	1	79	dep	Suaeda	308:313	arg1	monoica					315:321	Suaeda monoica	308:321	Suaeda monoica	308:321	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	0	80	theme	Plants	53:58	arg1	Compositions					14:25	Phytochemical Compositions	0:25	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.	0:102	Phytochemical Compositions of Some Red Sea Halophyte Plants with Antioxidant and Anticancer Potentials.
35684352	1	81	theme	Sea	251:253	arg1	album					340:344	Zygophyllum album	328:344	Zygophyllum album	328:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	81	theme	Sea	251:253	arg1	Suaeda					308:313	Suaeda	308:313	Suaeda	308:313	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	81	theme	Sea	251:253	arg1	Anabasis					267:274	Anabasis	267:274	Anabasis	267:274	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	81	theme	Sea	251:253	arg1	Suaeda					289:294	Suaeda	289:294	Suaeda	289:294	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	1	81	theme	Sea	251:253	arg1	halophytes					255:264	four Rea Sea halophytes	242:264	four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album	242:344	The aim of this study was to determine the compositions of carbohydrates, phenolic compounds, fatty acids (FAs), and amino acids (AAs) of four Rea Sea halophytes: Anabasis ehrenbergii, Suaeda aegyptiaca, Suaeda monoica, and Zygophyllum album.
35684352	7	82	theme	potent	1561:1566	arg1	activity					1578:1585	potent cytotoxic activity	1561:1585	potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines	1561:1636	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	7	83	theme	Z.	1542:1543	arg1	album					1545:1549	Z. album	1542:1549	Z. album	1542:1549	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	3	84	from	mannose	629:635	arg1	aegyptiaca					652:661	S. aegyptiaca	649:661	S. aegyptiaca	649:661	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	84	from	mannose	629:635	arg1	monoica					716:722	S. monoica	713:722	S. monoica	713:722	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	84	from	mannose	629:635	arg1	album					790:794	Z. album	787:794	Z. album	787:794	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	3	84	from	mannose	629:635	arg1	ehrenbergii					616:626	A. ehrenbergii	613:626	A. ehrenbergii	613:626	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	5	85	theme	palmitic	1100:1107	arg1	acid					1167:1170	the most abundant saturated fatty acid	1133:1170	the most abundant saturated fatty acid (SFA)	1133:1176	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	85	theme	palmitic	1100:1107	arg1	%					1126:1126	23.94-49.49%	1115:1126	23.94-49.49%	1115:1126	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	85	theme	palmitic	1100:1107	arg1	acid					1109:1112	palmitic acid	1100:1112	palmitic acid (23.94-49.49%)	1100:1127	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	3	86	dep	glucose	664:670	arg1	%					693:693	33 and 26%	684:693	33 and 26%	684:693	The polysaccharides of the four species were mainly composed of galactose (54.74%) in A. ehrenbergii, mannose (44.15%) in S. aegyptiaca, glucose and ribose (33 and 26%, respectively) in S. monoica, and arabinose and glucose (36.67 and 31.52%, respectively) in Z. album.
35684352	7	87	theme	album	1545:1549	arg1	fraction					1530:1537	the FAs fraction	1522:1537	the FAs fraction of Z. album	1522:1549	The phenolic fraction of S. aegyptiaca and S. monoica demonstrated noteworthy antioxidant activity with IC50 values of 9.0 and 8.0 μg/mL, respectively, while the FAs fraction of Z. album exhibited potent cytotoxic activity against Huh-7, A-549, and Caco-2 cancer cell lines with IC50 values of 7.4, 10.8, and 11.8 μg/mL, respectively.
35684352	8	88	theme	compounds	1788:1796	arg1	suitable					1852:1859	suitable	1852:1859	suitable	1852:1859	Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
35684352	8	88	theme	compounds	1788:1796	arg1	source					1758:1763	a source	1756:1763	a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications	1756:1883	Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
35684352	4	89	theme	halophyte	855:863	arg1	species					865:871	all halophyte species	851:871	all halophyte species	851:871	Glutamic acid and aspartic acid were the major AAs in all halophyte species with 50-63% and 10-22% of the total AAs, respectively.
35684352	8	90	theme	antioxidant	1803:1813	arg1	effects					1830:1836	antioxidant and anticancer effects	1803:1836	antioxidant and anticancer effects	1803:1836	Our results indicate that these plants may be considered a source of naturally occurring compounds with antioxidant and anticancer effects that could be suitable for future applications.
35684352	5	91	theme	species	988:994	arg1	UFA					971:973	UFA	971:973	UFA	971:973	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
35684352	5	91	theme	species	988:994	arg1	acids					964:968	unsaturated fatty acids	946:968	unsaturated fatty acids (UFA) of the four species	946:994	The proportion of unsaturated fatty acids (UFA) of the four species was 42.18-55.33%, comprised mainly of linolenic acid (15.54-28.63%) and oleic acid (5.68-22.05%), while palmitic acid (23.94-49.49%) was the most abundant saturated fatty acid (SFA).
36139844	6	0	dep	Fourier	916:922	arg1	transform					924:932	transform	924:932	transform infrared spectroscopy and nuclear magnetic resonance	924:985	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	8	1	theme	high	1411:1414	arg1	value					1442:1446	the high medicinal and nutritional value	1407:1446	the high medicinal and nutritional value of this mushroom	1407:1463	The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.
36139844	6	2	theme	β	1029:1029	arg1	bonds					1042:1046	α and β glycosidic bonds	1023:1046	α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues	1023:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	5	3	theme	main	799:802	arg1	composition					810:820	The main sugar composition	795:820	The main sugar composition of LECP	795:828	The main sugar composition of LECP includes mannose, galactose, glucose, fucose and glucuronic acid.
36139844	4	4	theme	methyl	701:706	arg1	linoleate					708:716	methyl linoleate	701:716	methyl linoleate	701:716	Likewise, LECP inhibited the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde.
36139844	3	5	theme	antioxidant	348:358	arg1	activity					360:367	The antioxidant activity	344:367	The antioxidant activity of LECP	344:375	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	7	6	theme	initial	1280:1286	arg1	stages					1298:1303	the initial and final stages	1276:1303	the initial and final stages of lipid peroxidation	1276:1325	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	6	7	theme	β-D-Manp-	1115:1123	arg1	residues					1128:1135	β-D-Manp-(1→ residues	1115:1135	β-D-Manp-(1→ residues	1115:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	6	8	theme	α	1023:1023	arg1	bonds					1042:1046	α and β glycosidic bonds	1023:1046	α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues	1023:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	6	9	theme	residues	1128:1135	arg1	backbone					1055:1062	a backbone	1053:1062	a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues	1053:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	6	10	theme	1→	1125:1126	arg1	residues					1128:1135	β-D-Manp-(1→ residues	1115:1135	β-D-Manp-(1→ residues	1115:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	4	11	theme	malondialdehyde	778:792	arg1	formation					730:738	the formation	726:738	the formation of conjugated diene hydroperoxide and malondialdehyde	726:792	Likewise, LECP inhibited the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde.
36139844	6	12	theme	-α-D-Galp-	1097:1106	arg1	1→					1108:1109	α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→	1067:1109	1→	1108:1109	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	8	13	theme	LECP	1368:1371	arg1	activity					1352:1359	The natural antioxidant activity	1328:1359	The natural antioxidant activity of the LECP that was investigated	1328:1393	The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.
36139844	3	14	theme	superoxide	527:536	arg1	1,1'-diphenyl-2-picrylhydrazyl					427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	3	14	theme	superoxide	527:536	arg1	radicals					544:551	hydroxyl and superoxide anion radicals	514:551	radicals	544:551	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	6	15	theme	α-D-Glc	1067:1073	arg1	1→					1108:1109	α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→	1067:1109	1→	1108:1109	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	2	16	theme	hot	265:267	arg1	water					269:273	hot water	265:273	hot water	265:273	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	1	17	theme	active	162:167	arg1	polysaccharides					169:183	biologically active polysaccharides	149:183	biologically active polysaccharides	149:183	The fruiting bodies of edible mushrooms represent an important source of biologically active polysaccharides.
36139844	5	18	theme	LECP	825:828	arg1	composition					810:820	The main sugar composition	795:820	The main sugar composition of LECP	795:828	The main sugar composition of LECP includes mannose, galactose, glucose, fucose and glucuronic acid.
36139844	3	19	theme	EC50	574:577	arg1	values					579:584	EC50 values	574:584	EC50 values	574:584	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	6	20	theme	1→	1090:1091	arg1	1→					1108:1109	α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→	1067:1109	1→	1108:1109	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	1	21	theme	polysaccharides	169:183	arg1	source					139:144	an important source	126:144	an important source of biologically active polysaccharides	126:183	The fruiting bodies of edible mushrooms represent an important source of biologically active polysaccharides.
36139844	7	22	theme	reactive	1184:1191	arg1	species					1193:1199	all reactive species	1180:1199	all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation	1180:1325	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	2	23	theme	antioxidant	286:296	arg1	activities					314:323	their antioxidant and antiradical activities	280:323	their antioxidant and antiradical activities	280:323	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	4	24	theme	diene	754:758	arg1	hydroperoxide					760:772	conjugated diene hydroperoxide	743:772	conjugated diene hydroperoxide	743:772	Likewise, LECP inhibited the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde.
36139844	6	25	dep	1→	1108:1109	arg1	→6					1094:1095	α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→	1067:1109	→6	1094:1095	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	6	25	dep	1→	1108:1109	arg1	→6					1076:1077	α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→	1067:1109	→6	1076:1077	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	1	26	theme	fruiting	80:87	arg1	bodies					89:94	The fruiting bodies	76:94	The fruiting bodies of edible mushrooms	76:114	The fruiting bodies of edible mushrooms represent an important source of biologically active polysaccharides.
36139844	4	27	theme	lipid	671:675	arg1	peroxidation					677:688	the lipid peroxidation	667:688	the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde	667:792	Likewise, LECP inhibited the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde.
36139844	4	28	theme	conjugated	743:752	arg1	hydroperoxide					760:772	conjugated diene hydroperoxide	743:772	conjugated diene hydroperoxide	743:772	Likewise, LECP inhibited the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde.
36139844	7	29	theme	peroxidation	1314:1325	arg1	stages					1298:1303	the initial and final stages	1276:1303	the initial and final stages of lipid peroxidation	1276:1325	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	6	30	theme	-β-D-Glcp-	1079:1088	arg1	1→					1108:1109	α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→	1067:1109	1→	1108:1109	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	3	31	theme	LECP	372:375	arg1	activity					360:367	The antioxidant activity	344:367	The antioxidant activity of LECP	344:375	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	4	32	theme	hydroperoxide	760:772	arg1	formation					730:738	the formation	726:738	the formation of conjugated diene hydroperoxide and malondialdehyde	726:792	Likewise, LECP inhibited the lipid peroxidation induced in methyl linoleate through the formation of conjugated diene hydroperoxide and malondialdehyde.
36139844	2	33	dep	edodes	211:216	arg1	polysaccharides					224:238	crude polysaccharides	218:238	Lentinula edodes crude polysaccharides (LECP)	201:245	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	2	33	dep	edodes	211:216	arg1	LECP					241:244	LECP	241:244	LECP	241:244	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	5	34	theme	sugar	804:808	arg1	composition					810:820	The main sugar composition	795:820	The main sugar composition of LECP	795:828	The main sugar composition of LECP includes mannose, galactose, glucose, fucose and glucuronic acid.
36139844	3	35	theme	anion	538:542	arg1	1,1'-diphenyl-2-picrylhydrazyl					427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	3	35	theme	anion	538:542	arg1	radicals					544:551	hydroxyl and superoxide anion radicals	514:551	radicals	544:551	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	6	36	theme	1→	1108:1109	arg1	backbone					1055:1062	a backbone	1053:1062	a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues	1053:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	6	37	with	bonds	1042:1046	arg1	backbone					1055:1062	a backbone	1053:1062	a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues	1053:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	7	38	theme	final	1292:1296	arg1	stages					1298:1303	the initial and final stages	1276:1303	the initial and final stages of lipid peroxidation	1276:1325	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	0	39	theme	Novel	20:24	arg1	Source					26:31	a Novel Source	18:31	a Novel Source of Polysaccharides with Antioxidant Power	18:73	Lentinula edodes, a Novel Source of Polysaccharides with Antioxidant Power.
36139844	7	40	theme	concentration-dependent	1213:1235	arg1	manner					1237:1242	a concentration-dependent manner	1211:1242	a concentration-dependent manner	1211:1242	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	7	41	from	effect	1266:1271	arg1	stages					1298:1303	the initial and final stages	1276:1303	the initial and final stages of lipid peroxidation	1276:1325	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	3	42	theme	3-ethylbenzothiazoline-6-sulfonic	474:506	arg1	acid					508:511	3-ethylbenzothiazoline-6-sulfonic acid	474:511	3-ethylbenzothiazoline-6-sulfonic acid	474:511	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	0	43	theme	Polysaccharides	36:50	arg1	Source					26:31	a Novel Source	18:31	a Novel Source of Polysaccharides with Antioxidant Power	18:73	Lentinula edodes, a Novel Source of Polysaccharides with Antioxidant Power.
36139844	8	44	theme	antioxidant	1340:1350	arg1	activity					1352:1359	The natural antioxidant activity	1328:1359	The natural antioxidant activity of the LECP that was investigated	1328:1393	The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.
36139844	8	45	theme	nutritional	1430:1440	arg1	value					1442:1446	the high medicinal and nutritional value	1407:1446	the high medicinal and nutritional value of this mushroom	1407:1463	The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.
36139844	2	46	theme	crude	218:222	arg1	polysaccharides					224:238	crude polysaccharides	218:238	Lentinula edodes crude polysaccharides (LECP)	201:245	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	2	46	theme	crude	218:222	arg1	LECP					241:244	LECP	241:244	LECP	241:244	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	8	47	theme	natural	1332:1338	arg1	activity					1352:1359	The natural antioxidant activity	1328:1359	The natural antioxidant activity of the LECP that was investigated	1328:1393	The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.
36139844	1	48	theme	edible	99:104	arg1	mushrooms					106:114	edible mushrooms	99:114	edible mushrooms	99:114	The fruiting bodies of edible mushrooms represent an important source of biologically active polysaccharides.
36139844	3	49	theme	reactive	402:409	arg1	species					411:417	reactive species	402:417	reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals	402:551	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	3	49	theme	reactive	402:409	arg1	1,1'-diphenyl-2-picrylhydrazyl					427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	2	50	theme	Lentinula	201:209	arg1	edodes					211:216	Lentinula edodes	201:216	Lentinula edodes crude polysaccharides (LECP)	201:245	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	3	51	dep	2,2'-azino-bis	459:472	arg1	acid					508:511	3-ethylbenzothiazoline-6-sulfonic acid	474:511	3-ethylbenzothiazoline-6-sulfonic acid	474:511	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	1	52	theme	mushrooms	106:114	arg1	bodies					89:94	The fruiting bodies	76:94	The fruiting bodies of edible mushrooms	76:114	The fruiting bodies of edible mushrooms represent an important source of biologically active polysaccharides.
36139844	8	53	theme	medicinal	1416:1424	arg1	value					1442:1446	the high medicinal and nutritional value	1407:1446	the high medicinal and nutritional value of this mushroom	1407:1463	The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.
36139844	6	54	theme	magnetic	968:975	arg1	resonance					977:985	nuclear magnetic resonance	960:985	nuclear magnetic resonance	960:985	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	3	55	theme	hydroxyl	514:521	arg1	1,1'-diphenyl-2-picrylhydrazyl					427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	1,1'-diphenyl-2-picrylhydrazyl	427:456	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	3	55	theme	hydroxyl	514:521	arg1	radicals					544:551	hydroxyl and superoxide anion radicals	514:551	radicals	544:551	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	7	56	theme	protective	1255:1264	arg1	effect					1266:1271	a protective effect	1253:1271	a protective effect in the initial and final stages of lipid peroxidation	1253:1325	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	0	57	with	Polysaccharides	36:50	arg1	Power					69:73	Antioxidant Power	57:73	Antioxidant Power	57:73	Lentinula edodes, a Novel Source of Polysaccharides with Antioxidant Power.
36139844	2	58	theme	antiradical	302:312	arg1	activities					314:323	their antioxidant and antiradical activities	280:323	their antioxidant and antiradical activities	280:323	In this study, Lentinula edodes crude polysaccharides (LECP) were extracted in hot water, and their antioxidant and antiradical activities were investigated.
36139844	0	59	theme	Antioxidant	57:67	arg1	Power					69:73	Antioxidant Power	57:73	Antioxidant Power	57:73	Lentinula edodes, a Novel Source of Polysaccharides with Antioxidant Power.
36139844	8	60	theme	mushroom	1456:1463	arg1	value					1442:1446	the high medicinal and nutritional value	1407:1446	the high medicinal and nutritional value of this mushroom	1407:1463	The natural antioxidant activity of the LECP that was investigated strengthens the high medicinal and nutritional value of this mushroom.
36139844	7	61	theme	lipid	1308:1312	arg1	peroxidation					1314:1325	lipid peroxidation	1308:1325	lipid peroxidation	1308:1325	The results showed that LECP can scavenge all reactive species tested in a concentration-dependent manner and with a protective effect in the initial and final stages of lipid peroxidation.
36139844	1	62	theme	important	129:137	arg1	source					139:144	an important source	126:144	an important source of biologically active polysaccharides	126:183	The fruiting bodies of edible mushrooms represent an important source of biologically active polysaccharides.
36139844	5	63	theme	glucuronic	879:888	arg1	acid					890:893	glucuronic acid	879:893	glucuronic acid	879:893	The main sugar composition of LECP includes mannose, galactose, glucose, fucose and glucuronic acid.
36139844	6	64	dep	transform	924:932	arg1	infrared					934:941	infrared	934:941	transform infrared spectroscopy and nuclear magnetic resonance	924:985	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	6	65	theme	nuclear	960:966	arg1	resonance					977:985	nuclear magnetic resonance	960:985	nuclear magnetic resonance	960:985	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
36139844	3	66	theme	mg/mL	621:625	arg1	values					579:584	EC50 values	574:584	EC50 values	574:584	The antioxidant activity of LECP was investigated against reactive species such as 1,1'-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, hydroxyl and superoxide anion radicals, reducing power with EC50 values of 0.51, 0.52, 2.19, 3.59 and 1.73 mg/mL, respectively.
36139844	6	67	theme	glycosidic	1031:1040	arg1	bonds					1042:1046	α and β glycosidic bonds	1023:1046	α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues	1023:1135	Characterization by Fourier transform infrared spectroscopy and nuclear magnetic resonance determined that LECP was made up of α and β glycosidic bonds with a backbone of α-D-Glc, →6)-β-D-Glcp-(1→, →6)-α-D-Galp-(1→ and β-D-Manp-(1→ residues.
35733140	12	0	theme	gut	2040:2042	arg1	composition					2054:2064	gut microbial composition	2040:2064	gut microbial composition	2040:2064	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	6	1	theme	monosaccharide	942:955	arg1	composition					957:967	monosaccharide composition	942:967	monosaccharide composition	942:967	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	2	theme	human	1112:1116	arg1	fermentation					1124:1135	human fecal fermentation	1112:1135	human fecal fermentation	1112:1135	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	4	3	theme	RP	627:628	arg1	unknown					634:640	unknown	634:640	unknown	634:640	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	4	3	theme	RP	627:628	arg1	characteristics					608:622	the digestive behavior and fecal fermentation characteristics	562:622	the digestive behavior and fecal fermentation characteristics of RP	562:628	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	8	4	used	utilized	1439:1446	arg2	RPI					1417:1419	RPI	1417:1419	RPI	1417:1419	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	8	4	used	utilized	1439:1446	arg2	RP					1413:1414	the indigestible RP	1396:1414	the indigestible RP (RPI)	1396:1420	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	1	5	theme	diseases	265:272	arg1	treatment					235:243	the treatment	231:243	the treatment of gastrointestinal diseases	231:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	12	6	theme	promoted	2213:2220	arg1	production					2222:2231	the promoted production	2209:2231	the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids	2209:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	2	7	theme	tanguticum	288:297	arg1	RP					316:317	RP	316:317	RP	316:317	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	2	7	theme	tanguticum	288:297	arg1	polysaccharides					299:313	R. tanguticum polysaccharides	285:313	R. tanguticum polysaccharides (RP)	285:318	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	6	8	theme	microbial	1178:1186	arg1	composition					1188:1198	gut microbial composition	1174:1198	gut microbial composition	1174:1198	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	13	9	from	stage	2516:2520	arg1	microbiota					2475:2484	colonic microbiota	2467:2484	colonic microbiota in human feces at the initial stage of fermentation	2467:2536	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	5	10	dep	behavior	803:810	arg1	the					789:791	the	789:791	the	789:791	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	13	11	theme	initial	2508:2514	arg1	stage					2516:2520	the initial stage	2504:2520	the initial stage of fermentation	2504:2536	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	3	12	from	stomach	527:533	arg1	properties					507:516	their metabolic properties	491:516	their metabolic properties from the stomach to the intestine	491:550	Usually, the pharmacological activities of polysaccharides are closely correlated to their metabolic properties from the stomach to the intestine.
35733140	12	13	theme	beneficial	2115:2124	arg1	bacteria					2126:2133	several beneficial bacteria	2107:2133	several beneficial bacteria	2107:2133	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	13	theme	beneficial	2115:2124	arg1	Bifidobacterium					2164:2178	Bifidobacterium	2164:2178	Bifidobacterium	2164:2178	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	13	theme	beneficial	2115:2124	arg1	Megamonas					2185:2193	Megamonas	2185:2193	Megamonas	2185:2193	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	13	theme	beneficial	2115:2124	arg1	Bacteroides					2151:2161	genera Bacteroides	2144:2161	genera Bacteroides	2144:2161	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	5	14	theme	gastrointestinal	725:740	arg1	model					742:746	an in vitro simulated gastrointestinal model	703:746	an in vitro simulated gastrointestinal model	703:746	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	1	15	theme	traditional	198:208	arg1	medicine					218:225	the well known traditional Chinese medicine	183:225	the well known traditional Chinese medicine for the treatment of gastrointestinal diseases	183:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	14	16	theme	health	2614:2619	arg1	improvement					2588:2598	the improvement	2584:2598	the improvement of intestinal health	2584:2619	RP could be used as potential prebiotics for the improvement of intestinal health.
35733140	13	17	theme	human	2489:2493	arg1	feces					2495:2499	human feces	2489:2499	human feces	2489:2499	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	1	18	theme	medicine	218:225	arg1	BACKGROUND					133:142	BACKGROUND	133:142	BACKGROUND Rheum tanguticum	133:159	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	1	18	theme	medicine	218:225	arg1	one					176:178	one	176:178	one	176:178	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	1	18	theme	medicine	218:225	arg1	medicine					218:225	the well known traditional Chinese medicine	183:225	the well known traditional Chinese medicine for the treatment of gastrointestinal diseases	183:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	8	19	theme	human	1473:1477	arg1	feces					1479:1483	human feces	1473:1483	human feces	1473:1483	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	11	20	from	RPI	1935:1937	arg1	faster					1963:1968	faster	1963:1968	faster	1963:1968	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	11	20	from	RPI	1935:1937	arg1	utilization					1893:1903	the utilization	1889:1903	the utilization of arabinose and galactose in RPI by colonic bacteria	1889:1957	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	12	21	theme	relative	2084:2091	arg1	abundances					2093:2102	the relative abundances	2080:2102	the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas	2080:2193	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	6	22	from	changes	871:877	arg1	composition					957:967	monosaccharide composition	942:967	monosaccharide composition	942:967	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	22	from	changes	871:877	arg1	composition					1004:1014	chemical composition	995:1014	chemical composition	995:1014	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	22	from	changes	871:877	arg1	acids					1049:1053	short chain fatty acids	1031:1053	short chain fatty acids	1031:1053	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	22	from	changes	871:877	arg1	sugar					979:983	reducing sugar	970:983	reducing sugar released	970:992	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	22	from	changes	871:877	arg1	properties					898:907	physicochemical properties	882:907	physicochemical properties	882:907	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	22	from	changes	871:877	arg1	value					1020:1024	pH value	1017:1024	pH value	1017:1024	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	22	from	changes	871:877	arg1	weight					934:939	molecular weight	924:939	molecular weight	924:939	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	12	23	theme	butyric	2300:2306	arg1	acids					2308:2312	acetic, propionic, and butyric acids	2277:2312	acetic, propionic, and butyric acids	2277:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	11	24	theme	galacturonic	1983:1994	arg1	acid					1996:1999	galacturonic acid	1983:1999	galacturonic acid	1983:1999	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	1	25	theme	Rheum	144:148	arg1	BACKGROUND					133:142	BACKGROUND	133:142	BACKGROUND Rheum tanguticum	133:159	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	1	25	theme	Rheum	144:148	arg1	one					176:178	one	176:178	one	176:178	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	1	25	theme	Rheum	144:148	arg1	medicine					218:225	the well known traditional Chinese medicine	183:225	the well known traditional Chinese medicine for the treatment of gastrointestinal diseases	183:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	6	26	theme	fatty	1043:1047	arg1	acids					1049:1053	short chain fatty acids	1031:1053	short chain fatty acids	1031:1053	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	8	27	theme	in	1495:1496	arg1	fermentation					1504:1515	the in vitro fermentation	1491:1515	the in vitro fermentation	1491:1515	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	11	28	from	arabinose	1908:1916	arg1	RPI					1935:1937	RPI	1935:1937	RPI	1935:1937	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	6	29	theme	short	1031:1035	arg1	acids					1049:1053	short chain fatty acids	1031:1053	short chain fatty acids	1031:1053	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	0	30	from	characteristics	19:33	arg1	modulation					92:101	the modulation	88:101	the modulation of gut microbial composition	88:130	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	11	31	from	galactose	1922:1930	arg1	RPI					1935:1937	RPI	1935:1937	RPI	1935:1937	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	5	32	theme	digestive	793:801	arg1	behavior					803:810	digestive behavior	793:810	digestive behavior	793:810	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	5	33	dep	in	706:707	arg1	vitro					709:713	vitro	709:713	vitro	709:713	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	12	34	theme	short-chain	2244:2254	arg1	acids					2308:2312	acetic, propionic, and butyric acids	2277:2312	acetic, propionic, and butyric acids	2277:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	34	theme	short-chain	2244:2254	arg1	acids					2262:2266	several short-chain fatty acids	2236:2266	several short-chain fatty acids	2236:2266	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	6	35	theme	composition	1188:1198	arg1	modulation					1160:1169	the modulation	1156:1169	the modulation of gut microbial composition	1156:1198	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	7	36	dep	in	1284:1285	arg1	vitro					1287:1291	vitro	1287:1291	vitro	1287:1291	RESULTS The results revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties.
35733140	6	37	theme	pH	1017:1018	arg1	value					1020:1024	pH value	1017:1024	pH value	1017:1024	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	11	38	from	utilization	1893:1903	arg1	RPI					1935:1937	RPI	1935:1937	RPI	1935:1937	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	12	39	theme	acids	2262:2266	arg1	production					2222:2231	the promoted production	2209:2231	the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids	2209:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	0	40	theme	gut	106:108	arg1	composition					120:130	gut microbial composition	106:130	gut microbial composition	106:130	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	7	41	theme	physicochemical	1356:1370	arg1	properties					1372:1381	physicochemical properties	1356:1381	physicochemical properties	1356:1381	RESULTS The results revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties.
35733140	8	42	theme	initial	1537:1543	arg1	0-6 h					1565:1569	0-6 h	1565:1569	0-6 h	1565:1569	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	8	42	theme	initial	1537:1543	arg1	stage					1558:1562	the initial fermentation stage	1533:1562	the initial fermentation stage (0-6 h)	1533:1570	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	8	43	theme	indigestible	1400:1411	arg1	RPI					1417:1419	RPI	1417:1419	RPI	1417:1419	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	8	43	theme	indigestible	1400:1411	arg1	RP					1413:1414	the indigestible RP	1396:1414	the indigestible RP (RPI)	1396:1420	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	3	44	theme	polysaccharides	449:463	arg1	activities					435:444	the pharmacological activities	415:444	the pharmacological activities of polysaccharides	415:463	Usually, the pharmacological activities of polysaccharides are closely correlated to their metabolic properties from the stomach to the intestine.
35733140	0	45	theme	Fecal	0:4	arg1	characteristics					19:33	Fecal fermentation characteristics	0:33	Fecal fermentation characteristics of Rheum tanguticum polysaccharide	0:68	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	13	46	theme	gastrointestinal	2402:2417	arg1	tract					2419:2423	the human upper gastrointestinal tract	2386:2423	the human upper gastrointestinal tract	2386:2423	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	6	47	theme	in	1079:1080	arg1	digestion					1098:1106	in vitro simulated digestion	1079:1106	in vitro simulated digestion	1079:1106	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	5	48	dep	METHODS	680:686	arg1	carried					752:758	carried	752:758	was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP	748:855	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	10	49	dep	0.778 mg/mL	1719:1729	arg1	0.177					1710:1714	0.177	1710:1714	0.177	1710:1714	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	3	50	theme	metabolic	497:505	arg1	properties					507:516	their metabolic properties	491:516	their metabolic properties from the stomach to the intestine	491:550	Usually, the pharmacological activities of polysaccharides are closely correlated to their metabolic properties from the stomach to the intestine.
35733140	10	51	theme	fermentation	1755:1766	arg1	stage					1746:1750	the initial stage	1734:1750	the initial stage of fermentation	1734:1766	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	6	52	theme	RP	912:913	arg1	composition					957:967	monosaccharide composition	942:967	monosaccharide composition	942:967	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	52	theme	RP	912:913	arg1	composition					1004:1014	chemical composition	995:1014	chemical composition	995:1014	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	52	theme	RP	912:913	arg1	acids					1049:1053	short chain fatty acids	1031:1053	short chain fatty acids	1031:1053	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	52	theme	RP	912:913	arg1	sugar					979:983	reducing sugar	970:983	reducing sugar released	970:992	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	52	theme	RP	912:913	arg1	properties					898:907	physicochemical properties	882:907	physicochemical properties	882:907	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	52	theme	RP	912:913	arg1	value					1020:1024	pH value	1017:1024	pH value	1017:1024	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	52	theme	RP	912:913	arg1	weight					934:939	molecular weight	924:939	molecular weight	924:939	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	9	53	theme	fermentation	1583:1594	arg1	characteristics					1596:1610	The fecal fermentation characteristics	1573:1610	The fecal fermentation characteristics of RPI	1573:1617	The fecal fermentation characteristics of RPI were revealed.
35733140	4	54	theme	fermentation	595:606	arg1	unknown					634:640	unknown	634:640	unknown	634:640	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	4	54	theme	fermentation	595:606	arg1	characteristics					608:622	the digestive behavior and fecal fermentation characteristics	562:622	the digestive behavior and fecal fermentation characteristics of RP	562:628	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	10	55	theme	fermentation	1866:1877	arg1	stage					1857:1861	the end stage	1849:1861	the end stage of fermentation	1849:1877	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	10	56	theme	sugars	1678:1683	arg1	content					1658:1664	the content	1654:1664	the content of reducing sugars	1654:1683	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	0	57	theme	Rheum	38:42	arg1	polysaccharide					55:68	Rheum tanguticum polysaccharide	38:68	Rheum tanguticum polysaccharide	38:68	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	14	58	theme	potential	2559:2567	arg1	RP					2539:2540	RP	2539:2540	RP	2539:2540	RP could be used as potential prebiotics for the improvement of intestinal health.
35733140	14	58	theme	potential	2559:2567	arg1	prebiotics					2569:2578	potential prebiotics	2559:2578	potential prebiotics for the improvement of intestinal health	2559:2619	RP could be used as potential prebiotics for the improvement of intestinal health.
35733140	2	59	theme	due	355:357	arg1	attention					345:353	increasing attention	334:353	increasing attention due to their diversely pharmacological activities	334:403	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	5	60	theme	fermentation	822:833	arg1	characteristics					835:849	fecal fermentation characteristics	816:849	fecal fermentation characteristics	816:849	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	0	61	dep	Rheum	38:42	arg1	tanguticum					44:53	tanguticum	44:53	tanguticum	44:53	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	7	62	theme	simulated	1293:1301	arg1	conditions					1313:1322	the in vitro simulated digestion conditions	1280:1322	the in vitro simulated digestion conditions according to its stabilities in physicochemical properties	1280:1381	RESULTS The results revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties.
35733140	4	63	theme	digestive	566:574	arg1	behavior					576:583	digestive behavior	566:583	digestive behavior	566:583	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	2	64	theme	increasing	334:343	arg1	attention					345:353	increasing attention	334:353	increasing attention due to their diversely pharmacological activities	334:403	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	6	65	theme	possible	862:869	arg1	changes					871:877	The possible changes	858:877	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids,	858:1054	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	7	66	theme	in	1284:1285	arg1	conditions					1313:1322	the in vitro simulated digestion conditions	1280:1322	the in vitro simulated digestion conditions according to its stabilities in physicochemical properties	1280:1381	RESULTS The results revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties.
35733140	8	67	theme	colonic	1451:1457	arg1	microbiota					1459:1468	colonic microbiota	1451:1468	colonic microbiota in human feces after the in vitro fermentation, especially	1451:1527	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	13	68	from	study	2345:2349	arg1	Results					2327:2333	CONCLUSIONS Results	2315:2333	CONCLUSIONS Results from this study	2315:2349	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	2	69	theme	pharmacological	378:392	arg1	activities					394:403	their diversely pharmacological activities	362:403	their diversely pharmacological activities	362:403	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	6	70	theme	fecal	1118:1122	arg1	fermentation					1124:1135	human fecal fermentation	1112:1135	human fecal fermentation	1112:1135	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	1	71	theme	gastrointestinal	248:263	arg1	diseases					265:272	gastrointestinal diseases	248:272	gastrointestinal diseases	248:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	14	72	used	used	2551:2554	arg2	RP					2539:2540	RP	2539:2540	RP	2539:2540	RP could be used as potential prebiotics for the improvement of intestinal health.
35733140	14	72	used	used	2551:2554	arg2	prebiotics					2569:2578	potential prebiotics	2559:2578	potential prebiotics for the improvement of intestinal health	2559:2619	RP could be used as potential prebiotics for the improvement of intestinal health.
35733140	13	73	theme	fermentation	2525:2536	arg1	stage					2516:2520	the initial stage	2504:2520	the initial stage of fermentation	2504:2536	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	6	74	theme	molecular	924:932	arg1	weight					934:939	molecular weight	924:939	molecular weight	924:939	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	1	75	used	utilized	164:171	arg2	medicine					218:225	the well known traditional Chinese medicine	183:225	the well known traditional Chinese medicine for the treatment of gastrointestinal diseases	183:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	1	75	used	utilized	164:171	arg2	one					176:178	one	176:178	one	176:178	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	1	75	used	utilized	164:171	arg2	BACKGROUND					133:142	BACKGROUND	133:142	BACKGROUND Rheum tanguticum	133:159	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	2	76	theme	R.	285:286	arg1	RP					316:317	RP	316:317	RP	316:317	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	2	76	theme	R.	285:286	arg1	polysaccharides					299:313	R. tanguticum polysaccharides	285:313	R. tanguticum polysaccharides (RP)	285:318	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	0	77	theme	polysaccharide	55:68	arg1	characteristics					19:33	Fecal fermentation characteristics	0:33	Fecal fermentation characteristics of Rheum tanguticum polysaccharide	0:68	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	0	77	theme	polysaccharide	55:68	arg1	effect					78:83	its effect	74:83	its effect on the modulation of gut microbial composition	74:130	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	6	78	theme	gut	1174:1176	arg1	composition					1188:1198	gut microbial composition	1174:1198	gut microbial composition	1174:1198	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	10	79	dep	0.828 × 105 Da	1831:1844	arg1	to					1828:1829	to	1828:1829	to	1828:1829	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	8	80	from	microbiota	1459:1468	arg1	feces					1479:1483	human feces	1473:1483	human feces	1473:1483	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	12	81	theme	genera	2144:2149	arg1	Bacteroides					2151:2161	genera Bacteroides	2144:2161	genera Bacteroides	2144:2161	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	5	82	theme	simulated	715:723	arg1	model					742:746	an in vitro simulated gastrointestinal model	703:746	an in vitro simulated gastrointestinal model	703:746	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	13	83	theme	human	2390:2394	arg1	tract					2419:2423	the human upper gastrointestinal tract	2386:2423	the human upper gastrointestinal tract	2386:2423	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	10	84	theme	molecular	1777:1785	arg1	weight					1787:1792	its molecular weight	1773:1792	its molecular weight	1773:1792	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	1	85	theme	known	192:196	arg1	medicine					218:225	the well known traditional Chinese medicine	183:225	the well known traditional Chinese medicine for the treatment of gastrointestinal diseases	183:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	6	86	theme	chemical	995:1002	arg1	composition					1004:1014	chemical composition	995:1014	chemical composition	995:1014	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	12	87	theme	several	2107:2113	arg1	bacteria					2126:2133	several beneficial bacteria	2107:2133	several beneficial bacteria	2107:2133	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	87	theme	several	2107:2113	arg1	Bifidobacterium					2164:2178	Bifidobacterium	2164:2178	Bifidobacterium	2164:2178	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	87	theme	several	2107:2113	arg1	Megamonas					2185:2193	Megamonas	2185:2193	Megamonas	2185:2193	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	87	theme	several	2107:2113	arg1	Bacteroides					2151:2161	genera Bacteroides	2144:2161	genera Bacteroides	2144:2161	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	1	88	theme	Chinese	210:216	arg1	medicine					218:225	the well known traditional Chinese medicine	183:225	the well known traditional Chinese medicine for the treatment of gastrointestinal diseases	183:272	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	14	89	theme	intestinal	2603:2612	arg1	health					2614:2619	intestinal health	2603:2619	intestinal health	2603:2619	RP could be used as potential prebiotics for the improvement of intestinal health.
35733140	0	90	from	effect	78:83	arg1	modulation					92:101	the modulation	88:101	the modulation of gut microbial composition	88:130	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	12	91	theme	bacteria	2126:2133	arg1	abundances					2093:2102	the relative abundances	2080:2102	the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas	2080:2193	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	10	92	theme	initial	1738:1744	arg1	stage					1746:1750	the initial stage	1734:1750	the initial stage of fermentation	1734:1766	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	6	93	theme	reducing	970:977	arg1	sugar					979:983	reducing sugar	970:983	reducing sugar released	970:992	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	13	94	theme	colonic	2467:2473	arg1	microbiota					2475:2484	colonic microbiota	2467:2484	colonic microbiota in human feces at the initial stage of fermentation	2467:2536	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	13	95	from	tract	2419:2423	arg1	indigestible					2370:2381	indigestible	2370:2381	indigestible	2370:2381	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	5	96	theme	in	706:707	arg1	model					742:746	an in vitro simulated gastrointestinal model	703:746	an in vitro simulated gastrointestinal model	703:746	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	10	97	theme	end	1853:1855	arg1	stage					1857:1861	the end stage	1849:1861	the end stage of fermentation	1849:1877	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	13	98	used	utilized	2455:2462	arg2	RP					2363:2364	RP	2363:2364	RP	2363:2364	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	6	99	theme	chain	1037:1041	arg1	acids					1049:1053	short chain fatty acids	1031:1053	short chain fatty acids	1031:1053	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	12	100	theme	propionic	2285:2293	arg1	acids					2308:2312	acetic, propionic, and butyric acids	2277:2312	acetic, propionic, and butyric acids	2277:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	101	theme	microbial	2044:2052	arg1	composition					2054:2064	gut microbial composition	2040:2064	gut microbial composition	2040:2064	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	0	102	theme	composition	120:130	arg1	modulation					92:101	the modulation	88:101	the modulation of gut microbial composition	88:130	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	12	103	theme	several	2236:2242	arg1	acids					2308:2312	acetic, propionic, and butyric acids	2277:2312	acetic, propionic, and butyric acids	2277:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	103	theme	several	2236:2242	arg1	acids					2262:2266	several short-chain fatty acids	2236:2266	several short-chain fatty acids	2236:2266	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	1	104	dep	Rheum	144:148	arg1	tanguticum					150:159	tanguticum	150:159	tanguticum	150:159	BACKGROUND Rheum tanguticum is utilized as one of the well known traditional Chinese medicine for the treatment of gastrointestinal diseases.
35733140	0	105	theme	microbial	110:118	arg1	composition					120:130	gut microbial composition	106:130	gut microbial composition	106:130	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
35733140	13	106	from	microbiota	2475:2484	arg1	feces					2495:2499	human feces	2489:2499	human feces	2489:2499	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	8	107	theme	fermentation	1545:1556	arg1	0-6 h					1565:1569	0-6 h	1565:1569	0-6 h	1565:1569	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	8	107	theme	fermentation	1545:1556	arg1	stage					1558:1562	the initial fermentation stage	1533:1562	the initial fermentation stage (0-6 h)	1533:1570	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	12	108	theme	fatty	2256:2260	arg1	acids					2308:2312	acetic, propionic, and butyric acids	2277:2312	acetic, propionic, and butyric acids	2277:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	12	108	theme	fatty	2256:2260	arg1	acids					2262:2266	several short-chain fatty acids	2236:2266	several short-chain fatty acids	2236:2266	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	11	109	theme	galactose	1922:1930	arg1	faster					1963:1968	faster	1963:1968	faster	1963:1968	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	11	109	theme	galactose	1922:1930	arg1	utilization					1893:1903	the utilization	1889:1903	the utilization of arabinose and galactose in RPI by colonic bacteria	1889:1957	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	5	110	theme	characteristics	835:849	arg1	investigation					772:784	the investigation	768:784	the investigation of the digestive behavior and fecal fermentation characteristics of RP	768:855	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	13	111	theme	upper	2396:2400	arg1	tract					2419:2423	the human upper gastrointestinal tract	2386:2423	the human upper gastrointestinal tract	2386:2423	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	4	112	theme	fecal	589:593	arg1	fermentation					595:606	fecal fermentation	589:606	fecal fermentation	589:606	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	6	113	dep	in	1079:1080	arg1	vitro					1082:1086	vitro	1082:1086	vitro	1082:1086	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	11	114	theme	arabinose	1908:1916	arg1	faster					1963:1968	faster	1963:1968	faster	1963:1968	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	11	114	theme	arabinose	1908:1916	arg1	utilization					1893:1903	the utilization	1889:1903	the utilization of arabinose and galactose in RPI by colonic bacteria	1889:1957	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	5	115	theme	RP	854:855	arg1	behavior					803:810	digestive behavior	793:810	digestive behavior	793:810	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	5	115	theme	RP	854:855	arg1	characteristics					835:849	fecal fermentation characteristics	816:849	fecal fermentation characteristics	816:849	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	8	116	dep	in	1495:1496	arg1	vitro					1498:1502	vitro	1498:1502	vitro	1498:1502	Conversely, the indigestible RP (RPI) could be notably utilized by colonic microbiota in human feces after the in vitro fermentation, especially, at the initial fermentation stage (0-6 h).
35733140	9	117	theme	fecal	1577:1581	arg1	characteristics					1596:1610	The fecal fermentation characteristics	1573:1610	The fecal fermentation characteristics of RPI	1573:1617	The fecal fermentation characteristics of RPI were revealed.
35733140	13	118	from	indigestible	2370:2381	arg1	tract					2419:2423	the human upper gastrointestinal tract	2386:2423	the human upper gastrointestinal tract	2386:2423	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	11	119	theme	colonic	1942:1948	arg1	bacteria					1950:1957	colonic bacteria	1942:1957	colonic bacteria	1942:1957	Notably, the utilization of arabinose and galactose in RPI by colonic bacteria was faster than that of galacturonic acid.
35733140	5	120	theme	behavior	803:810	arg1	investigation					772:784	the investigation	768:784	the investigation of the digestive behavior and fecal fermentation characteristics of RP	768:855	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	7	121	theme	digestion	1303:1311	arg1	conditions					1313:1322	the in vitro simulated digestion conditions	1280:1322	the in vitro simulated digestion conditions according to its stabilities in physicochemical properties	1280:1381	RESULTS The results revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties.
35733140	10	122	theme	reducing	1669:1676	arg1	sugars					1678:1683	reducing sugars	1669:1683	reducing sugars	1669:1683	Results showed that the content of reducing sugars obviously increased from 0.177 to 0.778 mg/mL at the initial stage of fermentation, and its molecular weight notably declined from 2.588 × 105 to 0.828 × 105 Da at the end stage of fermentation.
35733140	5	123	theme	fecal	816:820	arg1	characteristics					835:849	fecal fermentation characteristics	816:849	fecal fermentation characteristics	816:849	METHODS In this study, an in vitro simulated gastrointestinal model was carried out for the investigation of the digestive behavior and fecal fermentation characteristics of RP.
35733140	6	124	theme	physicochemical	882:896	arg1	composition					957:967	monosaccharide composition	942:967	monosaccharide composition	942:967	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	124	theme	physicochemical	882:896	arg1	composition					1004:1014	chemical composition	995:1014	chemical composition	995:1014	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	124	theme	physicochemical	882:896	arg1	acids					1049:1053	short chain fatty acids	1031:1053	short chain fatty acids	1031:1053	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	124	theme	physicochemical	882:896	arg1	sugar					979:983	reducing sugar	970:983	reducing sugar released	970:992	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	124	theme	physicochemical	882:896	arg1	properties					898:907	physicochemical properties	882:907	physicochemical properties	882:907	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	124	theme	physicochemical	882:896	arg1	value					1020:1024	pH value	1017:1024	pH value	1017:1024	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	6	124	theme	physicochemical	882:896	arg1	weight					934:939	molecular weight	924:939	molecular weight	924:939	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	9	125	theme	RPI	1615:1617	arg1	characteristics					1596:1610	The fecal fermentation characteristics	1573:1610	The fecal fermentation characteristics of RPI	1573:1617	The fecal fermentation characteristics of RPI were revealed.
35733140	12	126	theme	acetic	2277:2282	arg1	acids					2308:2312	acetic, propionic, and butyric acids	2277:2312	acetic, propionic, and butyric acids	2277:2312	Besides, RPI could obviously modulate gut microbial composition via promoting the relative abundances of several beneficial bacteria, such as genera Bacteroides, Bifidobacterium, and Megamonas, resulting in the promoted production of several short-chain fatty acids, such as acetic, propionic, and butyric acids.
35733140	3	127	theme	pharmacological	419:433	arg1	activities					435:444	the pharmacological activities	415:444	the pharmacological activities of polysaccharides	415:463	Usually, the pharmacological activities of polysaccharides are closely correlated to their metabolic properties from the stomach to the intestine.
35733140	4	128	theme	behavior	576:583	arg1	unknown					634:640	unknown	634:640	unknown	634:640	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	4	128	theme	behavior	576:583	arg1	characteristics					608:622	the digestive behavior and fecal fermentation characteristics	562:622	the digestive behavior and fecal fermentation characteristics of RP	562:628	However, the digestive behavior and fecal fermentation characteristics of RP are unknown, which need to be fully investigated.
35733140	6	129	from	effect	1146:1151	arg1	modulation					1160:1169	the modulation	1156:1169	the modulation of gut microbial composition	1156:1198	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	7	130	dep	RESULTS	1220:1226	arg1	revealed					1240:1247	revealed	1240:1247	revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties	1240:1381	RESULTS The results revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties.
35733140	2	131	theme	diversely	368:376	arg1	activities					394:403	their diversely pharmacological activities	362:403	their diversely pharmacological activities	362:403	Recently, R. tanguticum polysaccharides (RP) have received increasing attention due to their diversely pharmacological activities.
35733140	13	132	theme	CONCLUSIONS	2315:2325	arg1	Results					2327:2333	CONCLUSIONS Results	2315:2333	CONCLUSIONS Results from this study	2315:2349	CONCLUSIONS Results from this study showed that RP was indigestible in the human upper gastrointestinal tract in vitro, but could be easily utilized by colonic microbiota in human feces at the initial stage of fermentation.
35733140	7	133	from	stabilities	1341:1351	arg1	properties					1372:1381	physicochemical properties	1356:1381	physicochemical properties	1356:1381	RESULTS The results revealed that RP was indigestible under the in vitro simulated digestion conditions according to its stabilities in physicochemical properties.
35733140	6	134	theme	simulated	1088:1096	arg1	digestion					1098:1106	in vitro simulated digestion	1079:1106	in vitro simulated digestion	1079:1106	The possible changes in physicochemical properties of RP, such as molecular weight, monosaccharide composition, reducing sugar released, chemical composition, pH value, and short chain fatty acids, were determined during in vitro simulated digestion and human fecal fermentation, and its effect on the modulation of gut microbial composition was also evaluated.
35733140	0	135	theme	fermentation	6:17	arg1	characteristics					19:33	Fecal fermentation characteristics	0:33	Fecal fermentation characteristics of Rheum tanguticum polysaccharide	0:68	Fecal fermentation characteristics of Rheum tanguticum polysaccharide and its effect on the modulation of gut microbial composition.
36258024	13	0	theme	gene	2047:2050	arg1	expression					2052:2061	gene expression	2047:2061	gene expression in the midgut	2047:2075	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	15	1	theme	tryptophan	2700:2709	arg1	metabolism					2711:2720	tryptophan metabolism	2700:2720	tryptophan metabolism	2700:2720	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	16	2	theme	amino	2987:2991	arg1	metabolites					2974:2984	its metabolites	2970:2984	its metabolites	2970:2984	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	16	2	theme	amino	2987:2991	arg1	acid					2993:2996	amino acid	2987:2996	amino acid	2987:2996	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	10	3	theme	isolates	1686:1693	arg1	mixture					1695:1701	an indigenous isolates mixture	1672:1701	an indigenous isolates mixture	1672:1701	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	1	4	theme	vertebrate	227:236	arg1	heredity					238:245	vertebrate heredity	227:245	vertebrate heredity	227:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	5	5	dep	Bacillus	755:762	arg1	cereus					764:769	cereus	764:769	cereus	764:769	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	16	6	theme	enriched	2873:2880	arg1	pathways					2892:2899	enriched metabolic pathways	2873:2899	enriched metabolic pathways	2873:2899	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	3	7	from	habits	602:607	arg1	number					511:516	the large number	501:516	the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits	501:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	3	8	theme	large	505:509	arg1	number					511:516	the large number	501:516	the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits	501:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	15	9	theme	galactose	2678:2686	arg1	metabolism					2688:2697	galactose metabolism	2678:2697	galactose metabolism	2678:2697	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	17	10	dep	investigation	3092:3104	arg1	results					3106:3112	results	3106:3112	results	3106:3112	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	8	11	theme	diets	1213:1217	arg1	supplementation					1219:1233	diets supplementation	1213:1233	diets supplementation	1213:1233	Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control).
36258024	10	12	theme	digestive	1544:1552	arg1	amylase					1580:1586	amylase	1580:1586	amylase	1580:1586	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	10	12	theme	digestive	1544:1552	arg1	enzymes					1554:1560	digestive enzymes	1544:1560	digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities	1544:1632	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	10	12	theme	digestive	1544:1552	arg1	trypsin					1563:1569	trypsin	1563:1569	trypsin	1563:1569	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	10	12	theme	digestive	1544:1552	arg1	pepsin					1589:1594	pepsin	1589:1594	pepsin	1589:1594	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	10	12	theme	digestive	1544:1552	arg1	cellulose					1600:1608	cellulose	1600:1608	cellulose	1600:1608	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	10	12	theme	digestive	1544:1552	arg1	lipase					1572:1577	lipase	1572:1577	lipase	1572:1577	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	7	13	theme	juvenile	1042:1049	arg1	cobia					1051:1055	juvenile cobia	1042:1055	juvenile cobia	1042:1055	In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL-1 autochthonous strains for ten weeks and a control diet without supplementation.
36258024	3	14	from	studies	437:443	arg1	microbiota					457:466	fish gut microbiota	448:466	fish gut microbiota	448:466	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	9	15	theme	corpuscular	1427:1437	arg1	volume					1439:1444	mean corpuscular volume	1422:1444	mean corpuscular volume	1422:1444	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	15	theme	corpuscular	1427:1437	arg1	concentration					1407:1419	corpuscular haemoglobin concentration	1383:1419	corpuscular haemoglobin concentration	1383:1419	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	16	16	theme	heterocyclic	3019:3030	arg1	compounds					3032:3040	heterocyclic compounds	3019:3040	heterocyclic compounds	3019:3040	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	14	17	theme	supplemented	2361:2372	arg1	diet					2374:2377	supplemented diet	2361:2377	supplemented diet	2361:2377	Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains.
36258024	3	18	theme	varieties	526:534	arg1	number					511:516	the large number	501:516	the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits	501:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	16	19	from	differences	2858:2868	arg1	pathways					2892:2899	enriched metabolic pathways	2873:2899	enriched metabolic pathways	2873:2899	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	8	20	theme	improved	1259:1266	arg1	growth					1268:1273	significantly improved growth	1245:1273	significantly improved growth	1245:1273	Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control).
36258024	13	21	theme	factor	2113:2118	arg1	receptor					2120:2127	Colony-stimulating factor receptor 1	2094:2129	Colony-stimulating factor receptor 1	2094:2129	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	13	21	theme	factor	2113:2118	arg1	Immunoglobulin					2078:2091	Immunoglobulin	2078:2091	Immunoglobulin	2078:2091	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	2	22	theme	several	275:281	arg1	roles					283:287	several roles	275:287	several roles	275:287	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	17	23	theme	juvenile	3243:3250	arg1	cobia					3252:3256	juvenile cobia	3243:3256	juvenile cobia	3243:3256	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	0	24	from	profile	73:79	arg1	cobia					84:88	cobia	84:88	cobia (Rachycentron canadum)	84:111	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	0	24	from	profile	73:79	arg1	canadum					104:110	Rachycentron canadum	91:110	Rachycentron canadum	91:110	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	9	25	theme	haemoglobin	1395:1405	arg1	volume					1439:1444	mean corpuscular volume	1422:1444	mean corpuscular volume	1422:1444	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	25	theme	haemoglobin	1395:1405	arg1	concentration					1407:1419	corpuscular haemoglobin concentration	1383:1419	corpuscular haemoglobin concentration	1383:1419	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	25	theme	haemoglobin	1395:1405	arg1	haemoglobin					1447:1457	haemoglobin	1447:1457	haemoglobin	1447:1457	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	3	26	theme	conditions	563:572	arg1	number					511:516	the large number	501:516	the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits	501:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	5	27	theme	RCS3	771:774	arg1	mixture					776:782	Bacillus cereus RCS3 mixture	755:782	Bacillus cereus RCS3 mixture	755:782	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	16	28	theme	bacterial	2826:2834	arg1	community					2836:2844	bacterial community	2826:2844	bacterial community	2826:2844	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	4	29	theme	supplemented	664:675	arg1	strains					697:703	three autochthonous strains	677:703	supplemented three autochthonous strains	664:703	This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp.
36258024	4	29	theme	supplemented	664:675	arg1	sp					715:716	Bacillus sp	706:716	Bacillus sp	706:716	This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp.
36258024	1	30	from	evolution	214:222	arg1	heredity					238:245	vertebrate heredity	227:245	vertebrate heredity	227:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	3	31	theme	fluctuating	537:547	arg1	conditions					563:572	fluctuating environmental conditions	537:572	fluctuating environmental conditions	537:572	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	5	32	from	diet	784:787	arg1	fish					798:801	cobia fish	792:801	cobia fish (Rachycentron canadum)	792:824	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	5	32	from	diet	784:787	arg1	canadum					817:823	Rachycentron canadum	804:823	Rachycentron canadum	804:823	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	6	33	theme	high	871:874	arg1	sequencing					887:896	high throughput sequencing	871:896	high throughput sequencing	871:896	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	4	34	theme	autochthonous	683:695	arg1	strains					697:703	three autochthonous strains	677:703	supplemented three autochthonous strains	664:703	This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp.
36258024	4	34	theme	autochthonous	683:695	arg1	sp					715:716	Bacillus sp	706:716	Bacillus sp	706:716	This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp.
36258024	17	35	theme	cobia	3252:3256	arg1	growth					3187:3192	growth	3187:3192	growth	3187:3192	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	17	35	theme	cobia	3252:3256	arg1	survival					3195:3202	survival	3195:3202	survival	3195:3202	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	1	36	theme	fish	118:121	arg1	system					130:135	The fish immune system	114:135	The fish immune system	114:135	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	1	36	theme	fish	118:121	arg1	topic					142:146	topic	142:146	topic	142:146	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	10	37	theme	supplemented	1649:1660	arg1	diet					1662:1665	supplemented diet	1649:1665	supplemented diet with an indigenous isolates mixture	1649:1701	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	3	38	from	varieties	526:534	arg1	habits					602:607	feeding habits	594:607	feeding habits	594:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	11	39	theme	biochemical	1710:1720	arg1	albumin					1733:1739	albumin	1733:1739	albumin	1733:1739	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	39	theme	biochemical	1710:1720	arg1	protein					1762:1768	total protein	1756:1768	total protein	1756:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	39	theme	biochemical	1710:1720	arg1	parameters					1722:1731	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	39	theme	biochemical	1710:1720	arg1	globulin					1742:1749	globulin	1742:1749	globulin	1742:1749	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	15	40	theme	ABC	2785:2787	arg1	transporters					2789:2800	ABC transporters	2785:2800	ABC transporters	2785:2800	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	13	41	theme	histocompatibility	2242:2259	arg1	complex					2261:2267	Major histocompatibility complex 2	2236:2269	Major histocompatibility complex 2	2236:2269	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	6	42	theme	gut	961:963	arg1	microbiota					965:974	gut microbiota	961:974	gut microbiota	961:974	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	14	43	theme	bacterial	2309:2317	arg1	composition					2319:2329	Gut bacterial composition	2305:2329	Gut bacterial composition	2305:2329	Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains.
36258024	5	44	theme	Pantoea	725:731	arg1	RCS2					745:748	Pantoea agglomerans RCS2	725:748	Pantoea agglomerans RCS2	725:748	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	15	45	theme	metabolic	2444:2452	arg1	pathways					2454:2461	some metabolic pathways	2439:2461	some metabolic pathways	2439:2461	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	9	46	theme	supplemented	1513:1524	arg1	group					1526:1530	the supplemented group	1509:1530	the supplemented group	1509:1530	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	17	47	theme	current	3084:3090	arg1	investigation					3092:3104	The current investigation results	3080:3112	The current investigation results	3080:3112	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	7	48	theme	control	1150:1156	arg1	diet					1158:1161	a control diet	1148:1161	a control diet without supplementation	1148:1185	In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL-1 autochthonous strains for ten weeks and a control diet without supplementation.
36258024	5	49	theme	agglomerans	733:743	arg1	RCS2					745:748	Pantoea agglomerans RCS2	725:748	Pantoea agglomerans RCS2	725:748	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	2	50	theme	lipid	410:414	arg1	metabolism					416:425	lipid metabolism	410:425	lipid metabolism	410:425	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	0	51	from	mixture	33:39	arg1	profile					73:79	metabolic profile	63:79	metabolic profile	63:79	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	0	51	from	mixture	33:39	arg1	microbiota					48:57	gut microbiota	44:57	gut microbiota	44:57	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	13	52	theme	receptor	2217:2224	arg1	beta					2226:2229	T cell receptor beta	2210:2229	T cell receptor beta	2210:2229	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	15	53	theme	pathway	2526:2532	arg1	analysis					2534:2541	pathway analysis	2526:2541	pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment	2526:2608	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	10	54	with	diet	1662:1665	arg1	mixture					1695:1701	an indigenous isolates mixture	1672:1701	an indigenous isolates mixture	1672:1701	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	12	55	dep	higher	1999:2004	arg1	P < 0.05					1989:1996	P < 0.05	1989:1996	P < 0.05	1989:1996	On the other hand, glucose was significantly (P < 0.05) higher in the group without supplementation.
36258024	2	56	theme	energy	387:392	arg1	absorption					394:403	energy absorption	387:403	energy absorption	387:403	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	0	57	theme	autochthonous	11:23	arg1	strains					25:31	autochthonous strains	11:31	autochthonous strains	11:31	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	13	58	theme	major	2132:2136	arg1	complex					2157:2163	major histocompatibility complex 1	2132:2165	major histocompatibility complex 1	2132:2165	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	13	58	theme	major	2132:2136	arg1	Immunoglobulin					2078:2091	Immunoglobulin	2078:2091	Immunoglobulin	2078:2091	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	13	59	theme	Major	2236:2240	arg1	complex					2261:2267	Major histocompatibility complex 2	2236:2269	Major histocompatibility complex 2	2236:2269	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	6	60	from	composition	923:933	arg1	cobia					988:992	juvenile cobia	979:992	juvenile cobia fed with supplemented diet	979:1019	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	12	61	theme	other	1950:1954	arg1	hand					1956:1959	the other hand	1946:1959	the other hand	1946:1959	On the other hand, glucose was significantly (P < 0.05) higher in the group without supplementation.
36258024	3	62	from	conditions	563:572	arg1	habits					602:607	feeding habits	594:607	feeding habits	594:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	9	63	theme	red	1347:1349	arg1	cells					1357:1361	red blood cells	1347:1361	red blood cells	1347:1361	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	15	64	theme	purine	2762:2767	arg1	metabolism					2769:2778	purine metabolism	2762:2778	purine metabolism	2762:2778	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	1	65	dep	topic	142:146	arg1	a					140:140	a	140:140	a	140:140	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	3	66	theme	fish	448:451	arg1	microbiota					457:466	fish gut microbiota	448:466	fish gut microbiota	448:466	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	0	67	theme	gut	44:46	arg1	microbiota					48:57	gut microbiota	44:57	gut microbiota	44:57	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	13	68	theme	T	2210:2210	arg1	beta					2226:2229	T cell receptor beta	2210:2229	T cell receptor beta	2210:2229	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	11	69	theme	Serum	1704:1708	arg1	albumin					1733:1739	albumin	1733:1739	albumin	1733:1739	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	69	theme	Serum	1704:1708	arg1	protein					1762:1768	total protein	1756:1768	total protein	1756:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	69	theme	Serum	1704:1708	arg1	parameters					1722:1731	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	69	theme	Serum	1704:1708	arg1	globulin					1742:1749	globulin	1742:1749	globulin	1742:1749	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	70	theme	alanine	1817:1823	arg1	aminotransferase					1825:1840	alanine aminotransferase	1817:1840	alanine aminotransferase	1817:1840	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	9	71	theme	white	1364:1368	arg1	cells					1376:1380	white blood cells	1364:1380	white blood cells	1364:1380	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	11	72	theme	significant	1919:1929	arg1	difference					1931:1940	no significant difference	1916:1940	no significant difference	1916:1940	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	3	73	theme	practical	473:481	arg1	challenges					483:492	practical challenges	473:492	practical challenges	473:492	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	5	74	theme	Rachycentron	804:815	arg1	fish					798:801	cobia fish	792:801	cobia fish (Rachycentron canadum)	792:824	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	5	74	theme	Rachycentron	804:815	arg1	canadum					817:823	Rachycentron canadum	804:823	Rachycentron canadum	804:823	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	1	75	theme	system	207:212	arg1	evolution					214:222	defensive system evolution	197:222	defensive system evolution in vertebrate heredity	197:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	9	76	theme	corpuscular	1383:1393	arg1	volume					1439:1444	mean corpuscular volume	1422:1444	mean corpuscular volume	1422:1444	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	76	theme	corpuscular	1383:1393	arg1	concentration					1407:1419	corpuscular haemoglobin concentration	1383:1419	corpuscular haemoglobin concentration	1383:1419	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	76	theme	corpuscular	1383:1393	arg1	haemoglobin					1447:1457	haemoglobin	1447:1457	haemoglobin	1447:1457	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	6	77	theme	mass	849:852	arg1	spectrometry					854:865	mass spectrometry	849:865	mass spectrometry	849:865	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	15	78	theme	Genomes	2584:2590	arg1	enrichment					2599:2608	Genomes (KEGG) enrichment	2584:2608	Genomes (KEGG) enrichment	2584:2608	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	10	79	theme	enzymes	1554:1560	arg1	activities					1623:1632	digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities	1544:1632	digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities	1544:1632	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	10	80	theme	indigenous	1675:1684	arg1	isolates					1686:1693	indigenous isolates	1675:1693	an indigenous isolates mixture	1672:1701	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	14	81	theme	autochthonous	2384:2396	arg1	strains					2398:2404	autochthonous strains	2384:2404	autochthonous strains	2384:2404	Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains.
36258024	2	82	theme	gut	254:256	arg1	microbiota					258:267	gut microbiota	254:267	gut microbiota	254:267	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	9	83	theme	mean	1422:1425	arg1	volume					1439:1444	mean corpuscular volume	1422:1444	mean corpuscular volume	1422:1444	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	83	theme	mean	1422:1425	arg1	concentration					1407:1419	corpuscular haemoglobin concentration	1383:1419	corpuscular haemoglobin concentration	1383:1419	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	7	84	theme	1 × 1012 CFU mL-1	1090:1106	arg1	strains					1122:1128	1 × 1012 CFU mL-1 autochthonous strains	1090:1128	1 × 1012 CFU mL-1 autochthonous strains	1090:1128	In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL-1 autochthonous strains for ten weeks and a control diet without supplementation.
36258024	16	85	theme	metabolic	2882:2890	arg1	pathways					2892:2899	enriched metabolic pathways	2873:2899	enriched metabolic pathways	2873:2899	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	7	86	theme	trial	1029:1033	arg1	group					1035:1039	the trial group	1025:1039	the trial group	1025:1039	In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL-1 autochthonous strains for ten weeks and a control diet without supplementation.
36258024	3	87	theme	fish	521:524	arg1	varieties					526:534	fish varieties	521:534	fish varieties	521:534	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	1	88	from	heredity	238:245	arg1	understanding					180:192	a unique understanding	171:192	a unique understanding of defensive system evolution in vertebrate heredity	171:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	17	89	theme	autochthonous	3126:3138	arg1	mixture					3148:3154	autochthonous strains mixture	3126:3154	autochthonous strains mixture	3126:3154	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	11	90	theme	aspartate	1843:1851	arg1	aminotransferase					1853:1868	aspartate aminotransferase	1843:1868	aspartate aminotransferase	1843:1868	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	10	91	dep	trypsin	1563:1569	arg1	activities					1611:1620	activities	1611:1620	activities	1611:1620	Similarly, digestive enzymes (trypsin, lipase, amylase, pepsin and cellulose, activities) activities were higher in supplemented diet with an indigenous isolates mixture.
36258024	11	92	dep	parameters	1722:1731	arg1	albumin					1733:1739	albumin	1733:1739	albumin	1733:1739	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	92	dep	parameters	1722:1731	arg1	protein					1762:1768	total protein	1756:1768	total protein	1756:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	92	dep	parameters	1722:1731	arg1	parameters					1722:1731	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	92	dep	parameters	1722:1731	arg1	globulin					1742:1749	globulin	1742:1749	globulin	1742:1749	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	3	93	theme	environmental	549:561	arg1	conditions					563:572	fluctuating environmental conditions	537:572	fluctuating environmental conditions	537:572	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	15	94	theme	expressed	2639:2647	arg1	metabolites					2649:2659	differentially expressed metabolites	2624:2659	differentially expressed metabolites	2624:2659	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	5	95	theme	cobia	792:796	arg1	fish					798:801	cobia fish	792:801	cobia fish (Rachycentron canadum)	792:824	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	5	95	theme	cobia	792:796	arg1	canadum					817:823	Rachycentron canadum	804:823	Rachycentron canadum	804:823	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	16	96	theme	Functional	2803:2812	arg1	analysis					2814:2821	Functional analysis	2803:2821	Functional analysis of bacterial community	2803:2844	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	17	97	dep	growth	3187:3192	arg1	immunities					3229:3238	immunities	3229:3238	immunities	3229:3238	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	13	98	from	expression	2052:2061	arg1	midgut					2070:2075	the midgut	2066:2075	the midgut	2066:2075	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	1	99	from	understanding	180:192	arg1	heredity					238:245	vertebrate heredity	227:245	vertebrate heredity	227:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	9	100	theme	corpuscular	1469:1479	arg1	haemoglobin					1481:1491	mean corpuscular haemoglobin	1464:1491	mean corpuscular haemoglobin	1464:1491	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	8	101	theme	Juvenile	1188:1195	arg1	cobia					1197:1201	Juvenile cobia	1188:1201	Juvenile cobia receiving diets supplementation	1188:1233	Juvenile cobia receiving diets supplementation exhibited significantly improved growth than those without additives (control).
36258024	6	102	theme	throughput	876:885	arg1	sequencing					887:896	high throughput sequencing	871:896	high throughput sequencing	871:896	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	3	103	from	number	511:516	arg1	habits					602:607	feeding habits	594:607	feeding habits	594:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	3	104	theme	differences	579:589	arg1	number					511:516	the large number	501:516	the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits	501:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	15	105	theme	supplemented	2507:2518	arg1	diet					2520:2523	supplemented diet	2507:2523	supplemented diet	2507:2523	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	13	106	theme	significant	2281:2291	arg1	difference					2293:2302	no significant difference	2278:2302	no significant difference	2278:2302	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	6	107	theme	juvenile	979:986	arg1	cobia					988:992	juvenile cobia	979:992	juvenile cobia fed with supplemented diet	979:1019	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	16	108	theme	community	2836:2844	arg1	analysis					2814:2821	Functional analysis	2803:2821	Functional analysis of bacterial community	2803:2844	Functional analysis of bacterial community showed that differences in enriched metabolic pathways generally comprised carbohydrate and its metabolites, nucleotide and its metabolites, amino acid and its metabolites, heterocyclic compounds, and tryptamines, cholines, pigments.
36258024	0	109	theme	metabolic	63:71	arg1	profile					73:79	metabolic profile	63:79	metabolic profile	63:79	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	13	110	theme	Colony-stimulating	2094:2111	arg1	receptor					2120:2127	Colony-stimulating factor receptor 1	2094:2129	Colony-stimulating factor receptor 1	2094:2129	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	13	110	theme	Colony-stimulating	2094:2111	arg1	Immunoglobulin					2078:2091	Immunoglobulin	2078:2091	Immunoglobulin	2078:2091	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	1	111	theme	immune	123:128	arg1	system					130:135	The fish immune system	114:135	The fish immune system	114:135	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	1	111	theme	immune	123:128	arg1	topic					142:146	topic	142:146	topic	142:146	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	6	112	theme	microbiota	965:974	arg1	profile					950:956	metabolite profile	939:956	metabolite profile	939:956	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	6	112	theme	microbiota	965:974	arg1	composition					923:933	composition	923:933	composition	923:933	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	15	113	theme	carbohydrate	2723:2734	arg1	digestion					2736:2744	carbohydrate digestion	2723:2744	carbohydrate digestion	2723:2744	Metabolomics also revealed that some metabolic pathways were considerably enriched in fish fed with supplemented diet; pathway analysis based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment revealed that differentially expressed metabolites were involved in galactose metabolism, tryptophan metabolism, carbohydrate digestion and absorption, purine metabolism, and ABC transporters.
36258024	14	114	theme	Gut	2305:2307	arg1	composition					2319:2329	Gut bacterial composition	2305:2329	Gut bacterial composition	2305:2329	Gut bacterial composition was altered in fish receiving supplemented diet with autochthonous strains.
36258024	11	115	theme	total	1756:1760	arg1	protein					1762:1768	total protein	1756:1768	total protein	1756:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	11	115	theme	total	1756:1760	arg1	parameters					1722:1731	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein	1704:1768	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	17	116	theme	strains	3140:3146	arg1	mixture					3148:3154	autochthonous strains mixture	3126:3154	autochthonous strains mixture	3126:3154	The current investigation results showed that autochthonous strains mixture has significantly enhanced the growth, survival, and innate and adaptive immunities of juvenile cobia.
36258024	6	117	theme	metabolite	939:948	arg1	profile					950:956	metabolite profile	939:956	metabolite profile	939:956	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	0	118	from	microbiota	48:57	arg1	cobia					84:88	cobia	84:88	cobia (Rachycentron canadum)	84:111	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	0	118	from	microbiota	48:57	arg1	canadum					104:110	Rachycentron canadum	91:110	Rachycentron canadum	91:110	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	1	119	theme	defensive	197:205	arg1	evolution					214:222	defensive system evolution	197:222	defensive system evolution in vertebrate heredity	197:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	3	120	from	differences	579:589	arg1	habits					602:607	feeding habits	594:607	feeding habits	594:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	2	121	theme	bacterial	353:361	arg1	invasion					363:370	bacterial invasion	353:370	bacterial invasion	353:370	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	5	122	theme	Bacillus	755:762	arg1	mixture					776:782	Bacillus cereus RCS3 mixture	755:782	Bacillus cereus RCS3 mixture	755:782	RCS1, Pantoea agglomerans RCS2, and Bacillus cereus RCS3 mixture diet on cobia fish (Rachycentron canadum).
36258024	9	123	theme	Haematological	1315:1328	arg1	cells					1376:1380	white blood cells	1364:1380	white blood cells	1364:1380	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	123	theme	Haematological	1315:1328	arg1	cells					1357:1361	red blood cells	1347:1361	red blood cells	1347:1361	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	123	theme	Haematological	1315:1328	arg1	indices					1330:1336	Haematological indices	1315:1336	Haematological indices	1315:1336	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	123	theme	Haematological	1315:1328	arg1	concentration					1407:1419	corpuscular haemoglobin concentration	1383:1419	corpuscular haemoglobin concentration	1383:1419	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	123	theme	Haematological	1315:1328	arg1	haemoglobin					1481:1491	mean corpuscular haemoglobin	1464:1491	mean corpuscular haemoglobin	1464:1491	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	6	124	from	profile	950:956	arg1	cobia					988:992	juvenile cobia	979:992	juvenile cobia fed with supplemented diet	979:1019	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	4	125	theme	strains	697:703	arg1	impacts					653:659	the impacts	649:659	the impacts of supplemented three autochthonous strains, Bacillus sp	649:716	This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp.
36258024	0	126	theme	strains	25:31	arg1	Effects					0:6	Effects	0:6	Effects of autochthonous strains	0:31	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	6	127	theme	supplemented	1003:1014	arg1	diet					1016:1019	supplemented diet	1003:1019	supplemented diet	1003:1019	Also, chromatography, mass spectrometry and high throughput sequencing were combined to explore composition and metabolite profile of gut microbiota in juvenile cobia fed with supplemented diet.
36258024	2	128	theme	absorption	394:403	arg1	regulation					373:382	regulation	373:382	regulation of energy absorption	373:403	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	2	128	theme	absorption	394:403	arg1	resistance					339:348	resistance	339:348	resistance to bacterial invasion	339:370	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	2	128	theme	absorption	394:403	arg1	growth					331:336	growth	331:336	growth	331:336	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	2	128	theme	absorption	394:403	arg1	metabolism					416:425	lipid metabolism	410:425	lipid metabolism	410:425	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	2	128	theme	absorption	394:403	arg1	health					320:325	health	320:325	health	320:325	While gut microbiota plays several roles in fish: well-being, promoting health and growth, resistance to bacterial invasion, regulation of energy absorption, and lipid metabolism.
36258024	4	129	theme	Bacillus	706:713	arg1	strains					697:703	three autochthonous strains	677:703	supplemented three autochthonous strains	664:703	This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp.
36258024	4	129	theme	Bacillus	706:713	arg1	sp					715:716	Bacillus sp	706:716	Bacillus sp	706:716	This study was carried out to evaluate the impacts of supplemented three autochthonous strains, Bacillus sp.
36258024	11	130	theme	alkaline	1871:1878	arg1	phosphatase					1880:1890	alkaline phosphatase	1871:1890	alkaline phosphatase	1871:1890	Serum biochemical parameters albumin, globulin, and total protein were significantly higher, while triglyceride, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and cholesterol showed no significant difference.
36258024	7	131	theme	autochthonous	1108:1120	arg1	strains					1122:1128	1 × 1012 CFU mL-1 autochthonous strains	1090:1128	1 × 1012 CFU mL-1 autochthonous strains	1090:1128	In the trial group, juvenile cobia received diets supplemented with 1 × 1012 CFU mL-1 autochthonous strains for ten weeks and a control diet without supplementation.
36258024	1	132	theme	unique	173:178	arg1	understanding					180:192	a unique understanding	171:192	a unique understanding of defensive system evolution in vertebrate heredity	171:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36258024	3	133	theme	feeding	594:600	arg1	habits					602:607	feeding habits	594:607	feeding habits	594:607	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	0	134	theme	Rachycentron	91:102	arg1	cobia					84:88	cobia	84:88	cobia (Rachycentron canadum)	84:111	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	0	134	theme	Rachycentron	91:102	arg1	canadum					104:110	Rachycentron canadum	91:110	Rachycentron canadum	91:110	Effects of autochthonous strains mixture on gut microbiota and metabolic profile in cobia (Rachycentron canadum).
36258024	9	135	theme	blood	1351:1355	arg1	cells					1357:1361	red blood cells	1347:1361	red blood cells	1347:1361	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	3	136	theme	gut	453:455	arg1	microbiota					457:466	fish gut microbiota	448:466	fish gut microbiota	448:466	However, studies on fish gut microbiota face practical challenges due to the large number of fish varieties, fluctuating environmental conditions, and differences in feeding habits.
36258024	13	137	theme	cell	2212:2215	arg1	beta					2226:2229	T cell receptor beta	2210:2229	T cell receptor beta	2210:2229	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	13	138	theme	histocompatibility	2138:2155	arg1	complex					2157:2163	major histocompatibility complex 1	2132:2165	major histocompatibility complex 1	2132:2165	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	13	138	theme	histocompatibility	2138:2155	arg1	Immunoglobulin					2078:2091	Immunoglobulin	2078:2091	Immunoglobulin	2078:2091	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	9	139	theme	blood	1370:1374	arg1	cells					1376:1380	white blood cells	1364:1380	white blood cells	1364:1380	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	9	140	theme	mean	1464:1467	arg1	haemoglobin					1481:1491	mean corpuscular haemoglobin	1464:1491	mean corpuscular haemoglobin	1464:1491	Haematological indices, such as red blood cells, white blood cells, corpuscular haemoglobin concentration, mean corpuscular volume, haemoglobin, and mean corpuscular haemoglobin, were higher in the supplemented group.
36258024	13	141	theme	native	2188:2193	arg1	isolates					2195:2202	native isolates	2188:2202	native isolates	2188:2202	On gene expression in the midgut, Immunoglobulin, Colony-stimulating factor receptor 1, major histocompatibility complex 1 were up-regulated by native isolates while T cell receptor beta, and Major histocompatibility complex 2 showed no significant difference.
36258024	1	142	theme	evolution	214:222	arg1	understanding					180:192	a unique understanding	171:192	a unique understanding of defensive system evolution in vertebrate heredity	171:245	The fish immune system is a topic or subject that offers a unique understanding of defensive system evolution in vertebrate heredity.
36536589	12	0	theme	biochemical	1552:1562	arg1	sp					1617:1618	Desmodesmus sp	1605:1618	Desmodesmus sp	1605:1618	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	12	0	theme	biochemical	1552:1562	arg1	composition					1564:1574	the biochemical composition	1548:1574	the biochemical composition of mixed microalgae species	1548:1602	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	14	1	theme	partitioning	1668:1679	arg1	system					1687:1692	the liquid triphasic partitioning (TPP) system	1647:1692	the liquid triphasic partitioning (TPP) system	1647:1692	using the liquid triphasic partitioning (TPP) system.
36536589	8	2	theme	chlorophyll	1030:1040	arg1	concentration					1042:1054	chlorophyll concentration	1030:1054	chlorophyll concentration	1030:1054	The Green in the RGB index was the most promising way to estimate chlorophyll concentration in microalgae.
36536589	0	3	dep	bloom	135:139	arg1	Desmodesmus					48:58	Desmodesmus	48:58	Desmodesmus for nutrients	48:72	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	0	3	dep	bloom	135:139	arg1	partitioning					10:21	Triphasic partitioning	0:21	Triphasic partitioning of mixed Scenedesmus	0:42	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	15	4	theme	protein	1810:1816	arg1	concentrations					1777:1790	varied concentrations	1770:1790	varied concentrations of carbohydrates, protein, and lipids	1770:1828	The findings from analytical assays revealed that the biomass consisted of varied concentrations of carbohydrates, protein, and lipids.
36536589	14	5	theme	triphasic	1658:1666	arg1	system					1687:1692	the liquid triphasic partitioning (TPP) system	1647:1692	the liquid triphasic partitioning (TPP) system	1647:1692	using the liquid triphasic partitioning (TPP) system.
36536589	4	6	theme	inexpensive	508:518	arg1	method					520:525	an innovative and inexpensive method	490:525	an innovative and inexpensive method based on the red-green-blue (RGB) image analysis	490:574	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	9	7	theme	extractant	1177:1186	arg1	solvents					1188:1195	the four extractant solvents	1168:1195	the four extractant solvents	1168:1195	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	7	8	theme	independent	874:884	arg1	components					860:869	RGB colour components	849:869	RGB colour components	849:869	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	7	8	theme	independent	874:884	arg1	variables					886:894	independent variables	874:894	independent variables to estimate chlorophyll concentration	874:932	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	4	9	theme	image	561:565	arg1	analysis					567:574	the red-green-blue (RGB) image analysis	536:574	the red-green-blue (RGB) image analysis	536:574	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	0	10	theme	composition	102:112	arg1	prediction					114:123	chlorophyll composition prediction	90:123	chlorophyll composition prediction for algae	90:133	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	8	11	theme	RGB	981:983	arg1	index					985:989	the RGB index	977:989	the RGB index	977:989	The Green in the RGB index was the most promising way to estimate chlorophyll concentration in microalgae.
36536589	2	12	theme	chlorophyll	290:300	arg1	pigments					302:309	chlorophyll pigments	290:309	chlorophyll pigments	290:309	Therefore, the quantitation of chlorophyll pigments can be used as an indicator of algae bloom.
36536589	0	13	theme	chlorophyll	90:100	arg1	prediction					114:123	chlorophyll composition prediction	90:123	chlorophyll composition prediction for algae	90:133	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	5	14	theme	digital	655:661	arg1	images					663:668	The digital images	651:668	The digital images	651:668	The digital images were acquired using a smartphone camera.
36536589	15	15	theme	varied	1770:1775	arg1	concentrations					1777:1790	varied concentrations	1770:1790	varied concentrations of carbohydrates, protein, and lipids	1770:1828	The findings from analytical assays revealed that the biomass consisted of varied concentrations of carbohydrates, protein, and lipids.
36536589	2	16	theme	bloom	348:352	arg1	indicator					329:337	an indicator	326:337	an indicator of algae bloom	326:352	Therefore, the quantitation of chlorophyll pigments can be used as an indicator of algae bloom.
36536589	2	16	theme	bloom	348:352	arg1	quantitation					274:285	the quantitation	270:285	the quantitation of chlorophyll pigments	270:309	Therefore, the quantitation of chlorophyll pigments can be used as an indicator of algae bloom.
36536589	11	17	theme	bioactive	1424:1432	arg1	compounds					1434:1442	bioactive compounds	1424:1442	bioactive compounds	1424:1442	Microalgae are shown to produce bioactive compounds with a variety of biological activities that can be applied in various industries.
36536589	9	18	theme	extractant	1115:1124	arg1	acetone					1094:1100	acetone	1094:1100	acetone	1094:1100	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	9	18	theme	extractant	1115:1124	arg1	solvent					1126:1132	the best extractant solvent	1106:1132	the best extractant solvent	1106:1132	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	10	19	theme	regulating	1368:1377	arg1	algae					1379:1383	regulating algae bloom	1368:1389	regulating algae bloom	1368:1389	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	20	theme	biomolecules	1228:1239	arg1	alternative					1353:1363	an alternative	1350:1363	an alternative to regulating algae bloom	1350:1389	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	20	theme	biomolecules	1228:1239	arg1	isolation					1208:1216	the isolation	1204:1216	the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae,	1204:1325	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	11	21	theme	various	1507:1513	arg1	industries					1515:1524	various industries	1507:1524	various industries	1507:1524	Microalgae are shown to produce bioactive compounds with a variety of biological activities that can be applied in various industries.
36536589	6	22	theme	chlorophyll	782:792	arg1	concentration					794:806	chlorophyll concentration	782:806	chlorophyll concentration	782:806	The colour index was then evaluated using software and associated with chlorophyll concentration significantly.
36536589	7	23	theme	RGB	849:851	arg1	components					860:869	RGB colour components	849:869	RGB colour components	849:869	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	7	23	theme	RGB	849:851	arg1	variables					886:894	independent variables	874:894	independent variables to estimate chlorophyll concentration	874:932	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	1	24	theme	harmful	182:188	arg1	blooms					196:201	harmful algae blooms	182:201	harmful algae blooms that can affect the aquatic ecosystem and human health	182:256	Overgrowth of microalgae will result in harmful algae blooms that can affect the aquatic ecosystem and human health.
36536589	14	25	theme	liquid	1651:1656	arg1	system					1687:1692	the liquid triphasic partitioning (TPP) system	1647:1692	the liquid triphasic partitioning (TPP) system	1647:1692	using the liquid triphasic partitioning (TPP) system.
36536589	7	26	dep	variables	886:894	arg1	estimate					899:906	estimate	899:906	to estimate chlorophyll concentration	896:932	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	7	27	theme	colour	853:858	arg1	components					860:869	RGB colour components	849:869	RGB colour components	849:869	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	7	27	theme	colour	853:858	arg1	variables					886:894	independent variables	874:894	independent variables to estimate chlorophyll concentration	874:932	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	1	28	theme	algae	190:194	arg1	blooms					196:201	harmful algae blooms	182:201	harmful algae blooms that can affect the aquatic ecosystem and human health	182:256	Overgrowth of microalgae will result in harmful algae blooms that can affect the aquatic ecosystem and human health.
36536589	0	29	theme	Triphasic	0:8	arg1	partitioning					10:21	Triphasic partitioning	0:21	Triphasic partitioning of mixed Scenedesmus	0:42	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	15	30	from	assays	1724:1729	arg1	findings					1699:1706	The findings	1695:1706	The findings from analytical assays	1695:1729	The findings from analytical assays revealed that the biomass consisted of varied concentrations of carbohydrates, protein, and lipids.
36536589	3	31	theme	temporal	412:419	arg1	distribution					421:432	the geographical and temporal distribution	391:432	the geographical and temporal distribution of chlorophyll in the aquatic environment	391:474	However, it is difficult to monitor the geographical and temporal distribution of chlorophyll in the aquatic environment.
36536589	0	32	theme	mixed	26:30	arg1	Scenedesmus					32:42	mixed Scenedesmus	26:42	mixed Scenedesmus	26:42	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	4	33	theme	red-green-blue	540:553	arg1	analysis					567:574	the red-green-blue (RGB) image analysis	536:574	the red-green-blue (RGB) image analysis	536:574	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	8	34	from	Green	968:972	arg1	index					985:989	the RGB index	977:989	the RGB index	977:989	The Green in the RGB index was the most promising way to estimate chlorophyll concentration in microalgae.
36536589	9	35	theme	best	1110:1113	arg1	acetone					1094:1100	acetone	1094:1100	acetone	1094:1100	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	9	35	theme	best	1110:1113	arg1	solvent					1126:1132	the best extractant solvent	1106:1132	the best extractant solvent	1106:1132	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	15	36	theme	analytical	1713:1722	arg1	assays					1724:1729	analytical assays	1713:1729	analytical assays	1713:1729	The findings from analytical assays revealed that the biomass consisted of varied concentrations of carbohydrates, protein, and lipids.
36536589	5	37	theme	smartphone	692:701	arg1	camera					703:708	a smartphone camera	690:708	a smartphone camera	690:708	The digital images were acquired using a smartphone camera.
36536589	2	38	theme	pigments	302:309	arg1	indicator					329:337	an indicator	326:337	an indicator of algae bloom	326:352	Therefore, the quantitation of chlorophyll pigments can be used as an indicator of algae bloom.
36536589	2	38	theme	pigments	302:309	arg1	quantitation					274:285	the quantitation	270:285	the quantitation of chlorophyll pigments	270:309	Therefore, the quantitation of chlorophyll pigments can be used as an indicator of algae bloom.
36536589	7	39	theme	regression	825:834	arg1	model					836:840	A regression model	823:840	A regression model	823:840	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	3	40	theme	aquatic	456:462	arg1	environment					464:474	the aquatic environment	452:474	the aquatic environment	452:474	However, it is difficult to monitor the geographical and temporal distribution of chlorophyll in the aquatic environment.
36536589	4	41	theme	RGB	556:558	arg1	analysis					567:574	the red-green-blue (RGB) image analysis	536:574	the red-green-blue (RGB) image analysis	536:574	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	4	42	theme	chlorophyll	630:640	arg1	content					642:648	the microalgae chlorophyll content	615:648	the microalgae chlorophyll content	615:648	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	10	43	theme	useful	1221:1226	arg1	polysaccharides					1273:1287	polysaccharides	1273:1287	polysaccharides	1273:1287	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	43	theme	useful	1221:1226	arg1	proteins					1250:1257	proteins	1250:1257	proteins	1250:1257	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	43	theme	useful	1221:1226	arg1	acids					1266:1270	fatty acids	1260:1270	fatty acids	1260:1270	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	43	theme	useful	1221:1226	arg1	biomolecules					1228:1239	useful biomolecules	1221:1239	useful biomolecules	1221:1239	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	43	theme	useful	1221:1226	arg1	antioxidants					1293:1304	antioxidants	1293:1304	antioxidants	1293:1304	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	3	44	theme	chlorophyll	437:447	arg1	distribution					421:432	the geographical and temporal distribution	391:432	the geographical and temporal distribution of chlorophyll in the aquatic environment	391:474	However, it is difficult to monitor the geographical and temporal distribution of chlorophyll in the aquatic environment.
36536589	0	45	theme	Scenedesmus	32:42	arg1	Desmodesmus					48:58	Desmodesmus	48:58	Desmodesmus for nutrients	48:72	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	0	45	theme	Scenedesmus	32:42	arg1	partitioning					10:21	Triphasic partitioning	0:21	Triphasic partitioning of mixed Scenedesmus	0:42	Triphasic partitioning of mixed Scenedesmus and Desmodesmus for nutrients' extraction and chlorophyll composition prediction for algae bloom.
36536589	12	46	theme	microalgae	1585:1594	arg1	species					1596:1602	mixed microalgae species	1579:1602	mixed microalgae species	1579:1602	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	3	47	theme	geographical	395:406	arg1	distribution					421:432	the geographical and temporal distribution	391:432	the geographical and temporal distribution of chlorophyll in the aquatic environment	391:474	However, it is difficult to monitor the geographical and temporal distribution of chlorophyll in the aquatic environment.
36536589	4	48	theme	microalgae	619:628	arg1	content					642:648	the microalgae chlorophyll content	615:648	the microalgae chlorophyll content	615:648	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	12	49	theme	mixed	1579:1583	arg1	species					1596:1602	mixed microalgae species	1579:1602	mixed microalgae species	1579:1602	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	13	50	theme	Scenedesmus	1625:1635	arg1	sp					1637:1638	Scenedesmus sp	1625:1638	Scenedesmus sp	1625:1638	and Scenedesmus sp.
36536589	12	51	theme	Desmodesmus	1605:1615	arg1	sp					1617:1618	Desmodesmus sp	1605:1618	Desmodesmus sp	1605:1618	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	12	51	theme	Desmodesmus	1605:1615	arg1	composition					1564:1574	the biochemical composition	1548:1574	the biochemical composition of mixed microalgae species	1548:1602	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	4	52	used	utilized	580:587	arg2	method					520:525	an innovative and inexpensive method	490:525	an innovative and inexpensive method based on the red-green-blue (RGB) image analysis	490:574	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	9	53	theme	high	1141:1144	arg1	value					1156:1160	a high R-squared value	1139:1160	a high R-squared value among the four extractant solvents	1139:1195	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	15	54	theme	carbohydrates	1795:1807	arg1	concentrations					1777:1790	varied concentrations	1770:1790	varied concentrations of carbohydrates, protein, and lipids	1770:1828	The findings from analytical assays revealed that the biomass consisted of varied concentrations of carbohydrates, protein, and lipids.
36536589	12	55	theme	species	1596:1602	arg1	sp					1617:1618	Desmodesmus sp	1605:1618	Desmodesmus sp	1605:1618	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	12	55	theme	species	1596:1602	arg1	composition					1564:1574	the biochemical composition	1548:1574	the biochemical composition of mixed microalgae species	1548:1602	This study evaluates the biochemical composition of mixed microalgae species, Desmodesmus sp.
36536589	8	56	theme	promising	1004:1012	arg1	Green					968:972	The Green	964:972	The Green in the RGB index	964:989	The Green in the RGB index was the most promising way to estimate chlorophyll concentration in microalgae.
36536589	8	56	theme	promising	1004:1012	arg1	way					1014:1016	the most promising way	995:1016	the most promising way to estimate chlorophyll concentration in microalgae	995:1068	The Green in the RGB index was the most promising way to estimate chlorophyll concentration in microalgae.
36536589	9	57	theme	R-squared	1146:1154	arg1	value					1156:1160	a high R-squared value	1139:1160	a high R-squared value among the four extractant solvents	1139:1195	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	6	58	theme	colour	715:720	arg1	index					722:726	The colour index	711:726	The colour index	711:726	The colour index was then evaluated using software and associated with chlorophyll concentration significantly.
36536589	1	59	theme	aquatic	223:229	arg1	ecosystem					231:239	aquatic ecosystem	223:239	aquatic ecosystem	223:239	Overgrowth of microalgae will result in harmful algae blooms that can affect the aquatic ecosystem and human health.
36536589	16	60	theme	Phenolic	1831:1838	arg1	compounds					1840:1848	Phenolic compounds	1831:1848	Phenolic compounds	1831:1848	Phenolic compounds and antioxidant activity were at 60.22 mg/L and 90.69%, respectively.
36536589	14	61	theme	TPP	1682:1684	arg1	system					1687:1692	the liquid triphasic partitioning (TPP) system	1647:1692	the liquid triphasic partitioning (TPP) system	1647:1692	using the liquid triphasic partitioning (TPP) system.
36536589	9	62	with	solvent	1126:1132	arg1	value					1156:1160	a high R-squared value	1139:1160	a high R-squared value among the four extractant solvents	1139:1195	The result showed that acetone was the best extractant solvent with a high R-squared value among the four extractant solvents.
36536589	11	63	theme	biological	1462:1471	arg1	activities					1473:1482	biological activities	1462:1482	biological activities that can be applied in various industries	1462:1524	Microalgae are shown to produce bioactive compounds with a variety of biological activities that can be applied in various industries.
36536589	7	64	theme	chlorophyll	908:918	arg1	concentration					920:932	chlorophyll concentration	908:932	chlorophyll concentration	908:932	A regression model, using RGB colour components as independent variables to estimate chlorophyll concentration, was developed and validated.
36536589	11	65	theme	activities	1473:1482	arg1	variety					1451:1457	a variety	1449:1457	a variety of biological activities that can be applied in various industries	1449:1524	Microalgae are shown to produce bioactive compounds with a variety of biological activities that can be applied in various industries.
36536589	11	65	theme	activities	1473:1482	arg1	activities					1473:1482	biological activities	1462:1482	biological activities that can be applied in various industries	1462:1524	Microalgae are shown to produce bioactive compounds with a variety of biological activities that can be applied in various industries.
36536589	15	66	theme	lipids	1823:1828	arg1	concentrations					1777:1790	varied concentrations	1770:1790	varied concentrations of carbohydrates, protein, and lipids	1770:1828	The findings from analytical assays revealed that the biomass consisted of varied concentrations of carbohydrates, protein, and lipids.
36536589	10	67	from	microalgae	1315:1324	arg1	polysaccharides					1273:1287	polysaccharides	1273:1287	polysaccharides	1273:1287	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	67	from	microalgae	1315:1324	arg1	proteins					1250:1257	proteins	1250:1257	proteins	1250:1257	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	67	from	microalgae	1315:1324	arg1	acids					1266:1270	fatty acids	1260:1270	fatty acids	1260:1270	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	67	from	microalgae	1315:1324	arg1	antioxidants					1293:1304	antioxidants	1293:1304	antioxidants	1293:1304	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	10	68	dep	algae	1379:1383	arg1	bloom					1385:1389	bloom	1385:1389	bloom	1385:1389	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	16	69	theme	antioxidant	1854:1864	arg1	activity					1866:1873	antioxidant activity	1854:1873	antioxidant activity	1854:1873	Phenolic compounds and antioxidant activity were at 60.22 mg/L and 90.69%, respectively.
36536589	2	70	used	used	318:321	arg2	quantitation					274:285	the quantitation	270:285	the quantitation of chlorophyll pigments	270:309	Therefore, the quantitation of chlorophyll pigments can be used as an indicator of algae bloom.
36536589	2	70	used	used	318:321	arg2	indicator					329:337	an indicator	326:337	an indicator of algae bloom	326:352	Therefore, the quantitation of chlorophyll pigments can be used as an indicator of algae bloom.
36536589	4	71	theme	innovative	493:502	arg1	method					520:525	an innovative and inexpensive method	490:525	an innovative and inexpensive method based on the red-green-blue (RGB) image analysis	490:574	Accordingly, an innovative and inexpensive method based on the red-green-blue (RGB) image analysis was utilized in this study to estimate the microalgae chlorophyll content.
36536589	3	72	from	distribution	421:432	arg1	environment					464:474	the aquatic environment	452:474	the aquatic environment	452:474	However, it is difficult to monitor the geographical and temporal distribution of chlorophyll in the aquatic environment.
36536589	1	73	theme	microalgae	156:165	arg1	Overgrowth					142:151	Overgrowth	142:151	Overgrowth of microalgae	142:165	Overgrowth of microalgae will result in harmful algae blooms that can affect the aquatic ecosystem and human health.
36536589	10	74	theme	fatty	1260:1264	arg1	acids					1266:1270	fatty acids	1260:1270	fatty acids	1260:1270	Next, the isolation of useful biomolecules, such as proteins, fatty acids, polysaccharides and antioxidants from the microalgae, has been recognized as an alternative to regulating algae bloom.
36536589	1	75	theme	human	245:249	arg1	health					251:256	human health	245:256	human health	245:256	Overgrowth of microalgae will result in harmful algae blooms that can affect the aquatic ecosystem and human health.
37360290	0	0	theme	aquaculture	72:82	arg1	radiata					100:106	the novel aquaculture target Ecklonia radiata	62:106	the novel aquaculture target Ecklonia radiata	62:106	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	3	1	from	site	612:615	arg1	year					595:598	a full year	588:598	a full year at a single site (n = 138)	588:625	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	8	2	theme	viable	1602:1607	arg1	radiata					1583:1589	E. radiata	1580:1589	E. radiata	1580:1589	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	8	2	theme	viable	1602:1607	arg1	alternative					1629:1639	a viable southern hemisphere alternative	1600:1639	a viable southern hemisphere alternative for a broad range of commercial applications	1600:1684	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	5	3	theme	biomass	874:880	arg1	composition					882:892	The biomass composition	870:892	The biomass composition of E. radiata	870:906	The biomass composition of E. radiata varied significantly among sites but with no clear patterns among regions, indicating that geographic differences were mostly local rather than regional, possibly due to site-specific environmental conditions.
37360290	4	4	theme	High	628:631	arg1	variation					641:649	High spatial variation	628:649	High spatial variation	628:649	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	0	5	theme	novel	66:70	arg1	radiata					100:106	the novel aquaculture target Ecklonia radiata	62:106	the novel aquaculture target Ecklonia radiata	62:106	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	5	6	theme	E.	897:898	arg1	radiata					900:906	E. radiata	897:906	E. radiata	897:906	The biomass composition of E. radiata varied significantly among sites but with no clear patterns among regions, indicating that geographic differences were mostly local rather than regional, possibly due to site-specific environmental conditions.
37360290	6	7	dep	variation	1139:1147	arg1	measured					1150:1157	measured	1150:1157	measured by positive autocorrelation between months	1150:1200	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	3	8	theme	E.	479:480	arg1	biomass					490:496	E. radiata biomass	479:496	E. radiata biomass	479:496	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	0	9	theme	Ecklonia	91:98	arg1	radiata					100:106	the novel aquaculture target Ecklonia radiata	62:106	the novel aquaculture target Ecklonia radiata	62:106	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	5	10	theme	radiata	900:906	arg1	composition					882:892	The biomass composition	870:892	The biomass composition of E. radiata	870:906	The biomass composition of E. radiata varied significantly among sites but with no clear patterns among regions, indicating that geographic differences were mostly local rather than regional, possibly due to site-specific environmental conditions.
37360290	8	11	theme	hemisphere	1618:1627	arg1	radiata					1583:1589	E. radiata	1580:1589	E. radiata	1580:1589	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	8	11	theme	hemisphere	1618:1627	arg1	alternative					1629:1639	a viable southern hemisphere alternative	1600:1639	a viable southern hemisphere alternative for a broad range of commercial applications	1600:1684	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	2	12	theme	native	275:280	arg1	radiata					296:302	the native kelp Ecklonia radiata	271:302	the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry	271:395	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	6	13	dep	mannuronic	1342:1351	arg1	M					1372:1372	M	1372:1372	M:G	1372:1374	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	0	14	theme	target	84:89	arg1	radiata					100:106	the novel aquaculture target Ecklonia radiata	62:106	the novel aquaculture target Ecklonia radiata	62:106	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	4	15	dep	range	748:752	arg1	1.2					755:757	1.2	755:757	1.2	755:757	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	15	dep	range	748:752	arg1	%					764:764	1.6%	761:764	1.6% DW	761:767	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	15	dep	range	748:752	arg1	n = 12					770:775	n = 12	770:775	n = 12	770:775	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	8	16	theme	southern	1609:1616	arg1	radiata					1583:1589	E. radiata	1580:1589	E. radiata	1580:1589	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	8	16	theme	southern	1609:1616	arg1	alternative					1629:1639	a viable southern hemisphere alternative	1600:1639	a viable southern hemisphere alternative for a broad range of commercial applications	1600:1684	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	7	17	theme	higher	1520:1525	arg1	content					1540:1546	substantially higher phlorotannin content	1506:1546	substantially higher phlorotannin content	1506:1546	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	7	18	theme	comparable	1408:1417	arg1	composition					1427:1437	comparable biomass composition	1408:1437	comparable biomass composition	1408:1437	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	9	19	theme	supplementary	1741:1753	arg1	material					1755:1762	supplementary material	1741:1762	supplementary material available at 10.1007/s10811-023-02969-2	1741:1802	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	5	20	theme	environmental	1092:1104	arg1	conditions					1106:1115	site-specific environmental conditions	1078:1115	site-specific environmental conditions	1078:1115	The biomass composition of E. radiata varied significantly among sites but with no clear patterns among regions, indicating that geographic differences were mostly local rather than regional, possibly due to site-specific environmental conditions.
37360290	4	21	dep	range	838:842	arg1	9.3					845:847	9.3	845:847	9.3	845:847	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	21	dep	range	838:842	arg1	%					855:855	22.6%	851:855	22.6% DW	851:858	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	21	dep	range	838:842	arg1	n = 12					861:866	n = 12	861:866	n = 12	861:866	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	3	22	theme	temporal	438:445	arg1	variation					447:455	spatial and temporal variation	426:455	spatial and temporal variation	426:455	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	2	23	from	variation	208:216	arg1	quality					229:235	biomass quality	221:235	biomass quality	221:235	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	2	24	theme	emerging	359:366	arg1	industry					388:395	the emerging seaweed aquaculture industry	355:395	the emerging seaweed aquaculture industry	355:395	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	0	25	theme	radiata	100:106	arg1	composition					47:57	biomass composition	39:57	biomass composition of the novel aquaculture target Ecklonia radiata	39:106	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	3	26	from	variation	447:455	arg1	composition					464:474	the composition	460:474	the composition of E. radiata biomass	460:496	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	6	27	dep	M	1372:1372	arg1	G					1374:1374	G	1374:1374	M:G	1372:1374	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	9	28	from	10.1007/s10811-023-02969-2	1777:1802	arg1	available					1764:1772	available	1764:1772	available	1764:1772	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	0	29	theme	spatial	5:11	arg1	variation					26:34	High spatial and temporal variation	0:34	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.	0:107	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	0	30	from	variation	26:34	arg1	composition					47:57	biomass composition	39:57	biomass composition of the novel aquaculture target Ecklonia radiata	39:106	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	8	31	theme	broad	1647:1651	arg1	range					1653:1657	a broad range	1645:1657	a broad range of commercial applications	1645:1684	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	8	32	theme	E.	1580:1581	arg1	radiata					1583:1589	E. radiata	1580:1589	E. radiata	1580:1589	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	8	32	theme	E.	1580:1581	arg1	alternative					1629:1639	a viable southern hemisphere alternative	1600:1639	a viable southern hemisphere alternative for a broad range of commercial applications	1600:1684	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	4	33	theme	most	668:671	arg1	components					673:682	most components	668:682	most components	668:682	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	33	theme	most	668:671	arg1	glucose					829:835	glucose	829:835	glucose (range: 9.3 - 22.6% DW, n = 12)	829:867	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	33	theme	most	668:671	arg1	fucoidan					738:745	fucoidan	738:745	fucoidan (range: 1.2 - 1.6% DW, n = 12)	738:776	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	33	theme	most	668:671	arg1	alginate					695:702	alginate	695:702	alginate (range: 16.6 - 22.7% DW, n = 12)	695:735	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	33	theme	most	668:671	arg1	phlorotannins					779:791	phlorotannins	779:791	phlorotannins	779:791	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	0	34	theme	High	0:3	arg1	variation					26:34	High spatial and temporal variation	0:34	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.	0:107	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	3	35	theme	single	605:610	arg1	n = 138					618:624	n = 138	618:624	n = 138	618:624	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	3	35	theme	single	605:610	arg1	site					612:615	a single site	603:615	a single site (n = 138)	603:625	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	3	36	theme	North	534:538	arg1	Island					540:545	the North Island	530:545	the North Island of New Zealand	530:560	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	0	37	theme	temporal	17:24	arg1	variation					26:34	High spatial and temporal variation	0:34	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.	0:107	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	7	38	theme	E.	1393:1394	arg1	radiata					1396:1402	E. radiata	1393:1402	E. radiata	1393:1402	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	6	39	theme	temporal	1130:1137	arg1	variation					1139:1147	Significant temporal variation	1118:1147	Significant temporal variation (measured by positive autocorrelation between months)	1118:1201	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	6	40	theme	guluronic	1356:1364	arg1	acid					1366:1369	guluronic acid	1356:1369	guluronic acid	1356:1369	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	2	41	theme	Ecklonia	287:294	arg1	radiata					296:302	the native kelp Ecklonia radiata	271:302	the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry	271:395	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	4	42	theme	DW	725:726	arg1	%					723:723	22.7%	719:723	22.7% DW	719:726	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	6	43	theme	lipids	1234:1239	arg1	content					1223:1229	the content	1219:1229	the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury	1219:1327	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	6	44	theme	Significant	1118:1128	arg1	variation					1139:1147	Significant temporal variation	1118:1147	Significant temporal variation (measured by positive autocorrelation between months)	1118:1201	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	2	45	theme	kelp	282:285	arg1	radiata					296:302	the native kelp Ecklonia radiata	271:302	the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry	271:395	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	5	46	theme	site-specific	1078:1090	arg1	conditions					1106:1115	site-specific environmental conditions	1078:1115	site-specific environmental conditions	1078:1115	The biomass composition of E. radiata varied significantly among sites but with no clear patterns among regions, indicating that geographic differences were mostly local rather than regional, possibly due to site-specific environmental conditions.
37360290	3	47	theme	radiata	482:488	arg1	biomass					490:496	E. radiata biomass	479:496	E. radiata biomass	479:496	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	5	48	theme	clear	953:957	arg1	patterns					959:966	no clear patterns	950:966	no clear patterns among regions	950:980	The biomass composition of E. radiata varied significantly among sites but with no clear patterns among regions, indicating that geographic differences were mostly local rather than regional, possibly due to site-specific environmental conditions.
37360290	4	49	theme	spatial	633:639	arg1	variation					641:649	High spatial variation	628:649	High spatial variation	628:649	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	50	dep	range	794:798	arg1	4.8					801:803	4.8	801:803	4.8	801:803	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	50	dep	range	794:798	arg1	%					810:810	9.3%	807:810	9.3% DW	807:813	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	50	dep	range	794:798	arg1	n = 72					816:821	n = 72	816:821	n = 72	816:821	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	3	51	theme	New	550:552	arg1	Zealand					554:560	New Zealand	550:560	New Zealand	550:560	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	4	52	dep	range	705:709	arg1	16.6					712:715	16.6	712:715	16.6	712:715	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	52	dep	range	705:709	arg1	%					723:723	22.7%	719:723	22.7% DW	719:726	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	4	52	dep	range	705:709	arg1	n = 12					729:734	n = 12	729:734	n = 12	729:734	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	2	53	theme	aquaculture	376:386	arg1	industry					388:395	the emerging seaweed aquaculture industry	355:395	the emerging seaweed aquaculture industry	355:395	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	7	54	theme	northern	1469:1476	arg1	species					1489:1495	commercially grown northern hemisphere species	1450:1495	commercially grown northern hemisphere species	1450:1495	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	9	55	theme	Supplementary	1687:1699	arg1	Information					1701:1711	Supplementary Information	1687:1711	Supplementary Information The online version	1687:1730	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	8	56	theme	commercial	1662:1671	arg1	applications					1673:1684	commercial applications	1662:1684	commercial applications	1662:1684	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	7	57	theme	phlorotannin	1527:1538	arg1	content					1540:1546	substantially higher phlorotannin content	1506:1546	substantially higher phlorotannin content	1506:1546	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	2	58	theme	biomass	221:227	arg1	quality					229:235	biomass quality	221:235	biomass quality	221:235	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	6	59	located	detected	1207:1214	arg1	content					1223:1229	the content	1219:1229	the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury	1219:1327	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	6	59	located	detected	1207:1214	arg2	variation					1139:1147	Significant temporal variation	1118:1147	Significant temporal variation (measured by positive autocorrelation between months)	1118:1201	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	2	60	theme	seaweed	368:374	arg1	industry					388:395	the emerging seaweed aquaculture industry	355:395	the emerging seaweed aquaculture industry	355:395	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	8	61	theme	applications	1673:1684	arg1	range					1653:1657	a broad range	1645:1657	a broad range of commercial applications	1645:1684	These results demonstrate that E. radiata could be a viable southern hemisphere alternative for a broad range of commercial applications.
37360290	7	62	contain	had	1404:1406	arg2	composition					1427:1437	comparable biomass composition	1408:1437	comparable biomass composition	1408:1437	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	7	62	contain	had	1404:1406	arg1	radiata					1396:1402	E. radiata	1393:1402	E. radiata	1393:1402	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	3	63	theme	full	590:593	arg1	year					595:598	a full year	588:598	a full year at a single site (n = 138)	588:625	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	9	64	contain	contains	1732:1739	arg1	Information					1701:1711	Supplementary Information	1687:1711	Supplementary Information The online version	1687:1730	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	9	64	contain	contains	1732:1739	arg2	material					1755:1762	supplementary material	1741:1762	supplementary material available at 10.1007/s10811-023-02969-2	1741:1802	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	6	65	theme	mannuronic	1342:1351	arg1	ratio					1377:1381	the mannuronic to guluronic acid (M:G) ratio	1338:1381	the mannuronic to guluronic acid (M:G) ratio	1338:1381	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	7	66	theme	biomass	1419:1425	arg1	composition					1427:1437	comparable biomass composition	1408:1437	comparable biomass composition	1408:1437	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	0	67	theme	biomass	39:45	arg1	composition					47:57	biomass composition	39:57	biomass composition of the novel aquaculture target Ecklonia radiata	39:106	High spatial and temporal variation in biomass composition of the novel aquaculture target Ecklonia radiata.
37360290	1	68	theme	biomass	113:119	arg1	composition					121:131	The biomass composition	109:131	The biomass composition of kelp	109:139	The biomass composition of kelp varies within species both spatially and temporally.
37360290	4	69	theme	DW	857:858	arg1	%					855:855	22.6%	851:855	22.6% DW	851:858	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	3	70	theme	Zealand	554:560	arg1	Island					540:545	the North Island	530:545	the North Island of New Zealand	530:560	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	7	71	theme	hemisphere	1478:1487	arg1	species					1489:1495	commercially grown northern hemisphere species	1450:1495	commercially grown northern hemisphere species	1450:1495	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	4	72	theme	DW	766:767	arg1	%					764:764	1.6%	761:764	1.6% DW	761:767	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	9	73	theme	online	1717:1722	arg1	version					1724:1730	The online version	1713:1730	Supplementary Information The online version	1687:1730	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	9	74	dep	Information	1701:1711	arg1	version					1724:1730	The online version	1713:1730	Supplementary Information The online version	1687:1730	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	2	75	theme	New	311:313	arg1	Zealand					315:321	New Zealand	311:321	New Zealand	311:321	However, this variation in biomass quality has not yet been investigated for the native kelp Ecklonia radiata within New Zealand, where the kelp is a target for the emerging seaweed aquaculture industry.
37360290	6	76	theme	guluronic	1261:1269	arg1	acid					1271:1274	guluronic acid	1261:1274	guluronic acid	1261:1274	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	6	76	theme	guluronic	1261:1269	arg1	lipids					1234:1239	lipids	1234:1239	lipids	1234:1239	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	3	77	theme	spatial	426:432	arg1	variation					447:455	spatial and temporal variation	426:455	spatial and temporal variation	426:455	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
37360290	6	78	theme	positive	1162:1169	arg1	autocorrelation					1171:1185	positive autocorrelation	1162:1185	positive autocorrelation between months	1162:1200	Significant temporal variation (measured by positive autocorrelation between months) was detected in the content of lipids, proteins, glucose, guluronic acid, nitrogen, phosphorous, iodine, arsenic, and mercury, and for the mannuronic to guluronic acid (M:G) ratio.
37360290	9	79	theme	available	1764:1772	arg1	material					1755:1762	supplementary material	1741:1762	supplementary material available at 10.1007/s10811-023-02969-2	1741:1802	Supplementary Information The online version contains supplementary material available at 10.1007/s10811-023-02969-2.
37360290	1	80	theme	kelp	136:139	arg1	composition					121:131	The biomass composition	109:131	The biomass composition of kelp	109:139	The biomass composition of kelp varies within species both spatially and temporally.
37360290	7	81	theme	grown	1463:1467	arg1	species					1489:1495	commercially grown northern hemisphere species	1450:1495	commercially grown northern hemisphere species	1450:1495	Overall, E. radiata had comparable biomass composition to that of commercially grown northern hemisphere species but with substantially higher phlorotannin content.
37360290	5	82	theme	geographic	999:1008	arg1	differences					1010:1020	geographic differences	999:1020	geographic differences	999:1020	The biomass composition of E. radiata varied significantly among sites but with no clear patterns among regions, indicating that geographic differences were mostly local rather than regional, possibly due to site-specific environmental conditions.
37360290	4	83	theme	DW	812:813	arg1	%					810:810	9.3%	807:810	9.3% DW	807:813	High spatial variation was detected for most components, including alginate (range: 16.6 - 22.7% DW, n = 12), fucoidan (range: 1.2 - 1.6% DW, n = 12), phlorotannins (range: 4.8 - 9.3% DW, n = 72), and glucose (range: 9.3 - 22.6% DW, n = 12).
37360290	3	84	theme	biomass	490:496	arg1	composition					464:474	the composition	460:474	the composition of E. radiata biomass	460:496	In this study we quantified spatial and temporal variation in the composition of E. radiata biomass, collected from 12 sites around the North Island of New Zealand and from 12 months across a full year at a single site (n = 138).
36076830	0	0	theme	Antibiotic-Associated	78:98	arg1	Diarrhea					100:107	Antibiotic-Associated Diarrhea	78:107	Antibiotic-Associated Diarrhea in Mice	78:115	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	6	1	theme	BSP	867:869	arg1	administration					871:884	BSP administration	867:884	BSP administration	867:884	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	5	2	dep	intestine	741:749	arg1	the					731:733	the	731:733	the	731:733	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	3	3	theme	BSP	437:439	arg1	effects					426:432	The effects	422:432	The effects of BSP on mice with antibiotic-associated diarrhea (AAD)	422:489	The effects of BSP on mice with antibiotic-associated diarrhea (AAD) were investigated.
36076830	1	4	theme	water-soluble	167:179	arg1	BSP					196:198	A water-soluble polysaccharide BSP	165:198	A water-soluble polysaccharide BSP	165:198	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	1	5	theme	main	253:256	arg1	shoot					244:248	bamboo shoot	237:248	bamboo shoot	237:248	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	1	5	theme	main	253:256	arg1	by-product					258:267	a main by-product	251:267	a main by-product of bamboo shoot processing	251:294	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	3	6	with	mice	444:447	arg1	AAD					486:488	AAD	486:488	AAD	486:488	The effects of BSP on mice with antibiotic-associated diarrhea (AAD) were investigated.
36076830	3	6	with	mice	444:447	arg1	diarrhea					476:483	antibiotic-associated diarrhea	454:483	antibiotic-associated diarrhea (AAD)	454:489	The effects of BSP on mice with antibiotic-associated diarrhea (AAD) were investigated.
36076830	7	7	theme	shoot	1146:1150	arg1	by-products					1152:1162	bamboo shoot by-products	1139:1162	bamboo shoot by-products	1139:1162	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	7	8	theme	bamboo	1139:1144	arg1	by-products					1152:1162	bamboo shoot by-products	1139:1162	bamboo shoot by-products	1139:1162	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	5	9	from	intestine	741:749	arg1	mice					772:775	the AAD mice	764:775	the AAD mice	764:775	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	5	10	from	ratio	807:811	arg1	gut					820:822	the gut	816:822	the gut	816:822	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	7	11	theme	natural	1187:1193	arg1	component					1195:1203	an attractive natural component	1173:1203	an attractive natural component for gut health and AAD treatment	1173:1236	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	7	11	theme	natural	1187:1193	arg1	polysaccharides					1118:1132	the polysaccharides	1114:1132	the polysaccharides from bamboo shoot by-products	1114:1162	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	6	12	from	composition	899:909	arg1	mice					958:961	the AAD mice	950:961	the AAD mice	950:961	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	6	13	theme	AAD	954:956	arg1	mice					958:961	the AAD mice	950:961	the AAD mice	950:961	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	3	14	theme	antibiotic-associated	454:474	arg1	AAD					486:488	AAD	486:488	AAD	486:488	The effects of BSP on mice with antibiotic-associated diarrhea (AAD) were investigated.
36076830	3	14	theme	antibiotic-associated	454:474	arg1	diarrhea					476:483	antibiotic-associated diarrhea	454:483	antibiotic-associated diarrhea (AAD)	454:489	The effects of BSP on mice with antibiotic-associated diarrhea (AAD) were investigated.
36076830	4	15	theme	higher	597:602	arg1	concentrations					604:617	higher concentrations	597:617	higher concentrations of SCFAs	597:626	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	1	16	theme	polysaccharide	181:194	arg1	BSP					196:198	A water-soluble polysaccharide BSP	165:198	A water-soluble polysaccharide BSP	165:198	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	5	17	theme	small	735:739	arg1	intestine					741:749	small intestine	735:749	small intestine	735:749	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	4	18	theme	pH	584:585	arg1	value					587:591	lower pH value	578:591	lower pH value	578:591	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	6	19	from	diversity	915:923	arg1	mice					958:961	the AAD mice	950:961	the AAD mice	950:961	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	4	20	theme	lower	578:582	arg1	value					587:591	lower pH value	578:591	lower pH value	578:591	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	2	21	theme	arabinose	382:390	arg1	galactose					371:379	galactose	371:379	galactose	371:379	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	arabinose					382:390	arabinose	382:390	arabinose	382:390	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	xylose					333:338	xylose	333:338	xylose (19.43%)	333:347	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	glucose					316:322	glucose	316:322	glucose (72.8%)	316:330	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	amount					361:366	a small amount	353:366	a small amount of galactose, arabinose, glucuronic acid and mannose	353:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	acid					404:407	glucuronic acid	393:407	glucuronic acid	393:407	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	%					329:329	72.8%	325:329	72.8%	325:329	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	%					346:346	19.43%	341:346	19.43%	341:346	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	21	theme	arabinose	382:390	arg1	mannose					413:419	mannose	413:419	mannose	413:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	1	22	theme	bamboo	272:277	arg1	processing					285:294	bamboo shoot processing	272:294	bamboo shoot processing	272:294	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	3	23	from	effects	426:432	arg1	mice					444:447	mice	444:447	mice with antibiotic-associated diarrhea (AAD)	444:489	The effects of BSP on mice with antibiotic-associated diarrhea (AAD) were investigated.
36076830	7	24	theme	AAD	1224:1226	arg1	treatment					1228:1236	AAD treatment	1224:1236	AAD treatment	1224:1236	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	5	25	theme	Firmicutes/Bacteroidetes	782:805	arg1	ratio					807:811	Firmicutes/Bacteroidetes ratio	782:811	Firmicutes/Bacteroidetes ratio in the gut	782:822	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	0	26	theme	Shoot	28:32	arg1	Byproducts					57:66	Bamboo Shoot (Leleba oldhami Nakal) Byproducts	21:66	Bamboo Shoot (Leleba oldhami Nakal) Byproducts	21:66	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	1	27	theme	shoot	279:283	arg1	processing					285:294	bamboo shoot processing	272:294	bamboo shoot processing	272:294	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	5	28	dep	0.19	851:854	arg1	to					848:849	to	848:849	to	848:849	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	2	29	theme	galactose	371:379	arg1	galactose					371:379	galactose	371:379	galactose	371:379	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	arabinose					382:390	arabinose	382:390	arabinose	382:390	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	xylose					333:338	xylose	333:338	xylose (19.43%)	333:347	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	glucose					316:322	glucose	316:322	glucose (72.8%)	316:330	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	amount					361:366	a small amount	353:366	a small amount of galactose, arabinose, glucuronic acid and mannose	353:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	acid					404:407	glucuronic acid	393:407	glucuronic acid	393:407	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	%					329:329	72.8%	325:329	72.8%	325:329	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	%					346:346	19.43%	341:346	19.43%	341:346	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	29	theme	galactose	371:379	arg1	mannose					413:419	mannose	413:419	mannose	413:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	1	30	theme	processing	285:294	arg1	shoot					244:248	bamboo shoot	237:248	bamboo shoot	237:248	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	1	30	theme	processing	285:294	arg1	by-product					258:267	a main by-product	251:267	a main by-product of bamboo shoot processing	251:294	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	4	31	theme	SCFAs	622:626	arg1	value					587:591	lower pH value	578:591	lower pH value	578:591	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	4	31	theme	SCFAs	622:626	arg1	concentrations					604:617	higher concentrations	597:617	higher concentrations of SCFAs	597:626	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	4	31	theme	SCFAs	622:626	arg1	gain					572:575	significant higher bodyweight gain	542:575	significant higher bodyweight gain	542:575	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	4	32	theme	bodyweight	561:570	arg1	gain					572:575	significant higher bodyweight gain	542:575	significant higher bodyweight gain	542:575	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	2	33	theme	mannose	413:419	arg1	galactose					371:379	galactose	371:379	galactose	371:379	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	arabinose					382:390	arabinose	382:390	arabinose	382:390	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	xylose					333:338	xylose	333:338	xylose (19.43%)	333:347	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	glucose					316:322	glucose	316:322	glucose (72.8%)	316:330	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	amount					361:366	a small amount	353:366	a small amount of galactose, arabinose, glucuronic acid and mannose	353:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	acid					404:407	glucuronic acid	393:407	glucuronic acid	393:407	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	%					329:329	72.8%	325:329	72.8%	325:329	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	%					346:346	19.43%	341:346	19.43%	341:346	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	33	theme	mannose	413:419	arg1	mannose					413:419	mannose	413:419	mannose	413:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	0	34	theme	Gut	149:151	arg1	Microbiota					153:162	Gut Microbiota	149:162	Gut Microbiota	149:162	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	1	35	theme	basal	223:227	arg1	part					229:232	the basal part	219:232	the basal part of bamboo shoot, a main by-product of bamboo shoot processing	219:294	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	2	36	theme	small	355:359	arg1	arabinose					382:390	arabinose	382:390	arabinose	382:390	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	36	theme	small	355:359	arg1	acid					404:407	glucuronic acid	393:407	glucuronic acid	393:407	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	36	theme	small	355:359	arg1	galactose					371:379	galactose	371:379	galactose	371:379	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	36	theme	small	355:359	arg1	amount					361:366	a small amount	353:366	a small amount of galactose, arabinose, glucuronic acid and mannose	353:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	36	theme	small	355:359	arg1	mannose					413:419	mannose	413:419	mannose	413:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	5	37	theme	inflammatory	709:720	arg1	cells					722:726	the inflammatory cells	705:726	the inflammatory cells in the small intestine and colon in the AAD mice	705:775	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	0	38	from	Byproducts	57:66	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts	0:66	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	5	39	theme	AAD	768:770	arg1	mice					772:775	the AAD mice	764:775	the AAD mice	764:775	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	2	40	theme	acid	404:407	arg1	galactose					371:379	galactose	371:379	galactose	371:379	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	arabinose					382:390	arabinose	382:390	arabinose	382:390	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	xylose					333:338	xylose	333:338	xylose (19.43%)	333:347	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	glucose					316:322	glucose	316:322	glucose (72.8%)	316:330	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	amount					361:366	a small amount	353:366	a small amount of galactose, arabinose, glucuronic acid and mannose	353:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	acid					404:407	glucuronic acid	393:407	glucuronic acid	393:407	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	%					329:329	72.8%	325:329	72.8%	325:329	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	%					346:346	19.43%	341:346	19.43%	341:346	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	40	theme	acid	404:407	arg1	mannose					413:419	mannose	413:419	mannose	413:419	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	2	41	theme	glucuronic	393:402	arg1	acid					404:407	glucuronic acid	393:407	glucuronic acid	393:407	BSP is composed of glucose (72.8%), xylose (19.43%) and a small amount of galactose, arabinose, glucuronic acid and mannose.
36076830	6	42	dep	composition	899:909	arg1	the					895:897	the	895:897	the	895:897	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	7	43	theme	attractive	1176:1185	arg1	component					1195:1203	an attractive natural component	1173:1203	an attractive natural component for gut health and AAD treatment	1173:1236	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	7	43	theme	attractive	1176:1185	arg1	polysaccharides					1118:1132	the polysaccharides	1114:1132	the polysaccharides from bamboo shoot by-products	1114:1162	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	7	44	from	by-products	1152:1162	arg1	component					1195:1203	an attractive natural component	1173:1203	an attractive natural component for gut health and AAD treatment	1173:1236	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	7	44	from	by-products	1152:1162	arg1	polysaccharides					1118:1132	the polysaccharides	1114:1132	the polysaccharides from bamboo shoot by-products	1114:1162	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	5	45	from	colon	755:759	arg1	mice					772:775	the AAD mice	764:775	the AAD mice	764:775	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	4	46	dep	significant	542:552	arg1	higher					554:559	higher	554:559	higher	554:559	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
36076830	6	47	theme	gut	932:934	arg1	microbiota					936:945	the gut microbiota	928:945	the gut microbiota	928:945	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	6	48	theme	microbiota	936:945	arg1	diversity					915:923	diversity	915:923	diversity	915:923	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	6	48	theme	microbiota	936:945	arg1	composition					899:909	composition	899:909	composition	899:909	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	7	49	theme	gut	1209:1211	arg1	health					1213:1218	gut health	1209:1218	gut health	1209:1218	Our results suggest that the polysaccharides from bamboo shoot by-products could be an attractive natural component for gut health and AAD treatment.
36076830	0	50	with	Interactions	131:142	arg1	Microbiota					153:162	Gut Microbiota	149:162	Gut Microbiota	149:162	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	1	51	theme	bamboo	237:242	arg1	shoot					244:248	bamboo shoot	237:248	bamboo shoot	237:248	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	1	51	theme	bamboo	237:242	arg1	by-product					258:267	a main by-product	251:267	a main by-product of bamboo shoot processing	251:294	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	0	52	from	Diarrhea	100:107	arg1	Mice					112:115	Mice	112:115	Mice	112:115	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	6	53	theme	bacteria	1010:1017	arg1	improvement					984:994	the improvement	980:994	the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group	980:1086	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	1	54	theme	shoot	244:248	arg1	part					229:232	the basal part	219:232	the basal part of bamboo shoot, a main by-product of bamboo shoot processing	219:294	A water-soluble polysaccharide BSP was extracted from the basal part of bamboo shoot, a main by-product of bamboo shoot processing.
36076830	5	55	theme	BSP	678:680	arg1	administration					682:695	BSP administration	678:695	BSP administration	678:695	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	5	56	from	cells	722:726	arg1	colon					755:759	colon	755:759	colon	755:759	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	5	56	from	cells	722:726	arg1	intestine					741:749	small intestine	735:749	small intestine	735:749	BSP administration reduced the inflammatory cells in the small intestine and colon in the AAD mice, and Firmicutes/Bacteroidetes ratio in the gut was decreased from 0.56 to 0.19.
36076830	0	57	theme	Bamboo	21:26	arg1	Shoot					28:32	Bamboo Shoot	21:32	Bamboo Shoot (Leleba oldhami Nakal) Byproducts	21:66	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	0	57	theme	Bamboo	21:26	arg1	Nakal					50:54	Nakal	50:54	Nakal	50:54	Polysaccharides from Bamboo Shoot (Leleba oldhami Nakal) Byproducts Alleviate Antibiotic-Associated Diarrhea in Mice through Their Interactions with Gut Microbiota.
36076830	6	58	theme	beneficial	999:1008	arg1	Lachnospiraceae_NK4A136_group					1058:1086	Lachnospiraceae_NK4A136_group	1058:1086	Lachnospiraceae_NK4A136_group	1058:1086	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	6	58	theme	beneficial	999:1008	arg1	Lactobacillus					1040:1052	Lactobacillus	1040:1052	Lactobacillus	1040:1052	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	6	58	theme	beneficial	999:1008	arg1	bacteria					1010:1017	beneficial bacteria	999:1017	beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group	999:1086	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	6	58	theme	beneficial	999:1008	arg1	Bacteroides					1027:1037	Bacteroides	1027:1037	Bacteroides	1027:1037	Moreover, BSP administration affected the composition and diversity of the gut microbiota in the AAD mice, particularly on the improvement of beneficial bacteria such as Bacteroides, Lactobacillus and Lachnospiraceae_NK4A136_group.
36076830	4	59	theme	significant	542:552	arg1	gain					572:575	significant higher bodyweight gain	542:575	significant higher bodyweight gain	542:575	The mice fed with BSP exhibited significant higher bodyweight gain, lower pH value and higher concentrations of SCFAs in the feces compared with those fed with saline.
37123066	0	0	theme	polysaccharides	88:102	arg1	properties					61:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties	0:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides	0:102	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes.
37123066	3	1	dep	caused	717:722	arg1	destroyed					765:773	destroyed	765:773	caused LEP color changes and surface topography destroyed	717:773	Moreover, γ-irradiation treatment caused LEP color changes and surface topography destroyed.
37123066	1	2	with	influence	156:164	arg1	dose					198:201	different dose	188:201	different dose (0, 4, 8, and 16 kGy)	188:223	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	5	3	theme	γ-irradiated	883:894	arg1	LEP					896:898	γ-irradiated LEP	883:898	γ-irradiated LEP	883:898	Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum.
37123066	0	4	theme	γ-irradiated	75:86	arg1	polysaccharides					88:102	γ-irradiated polysaccharides	75:102	γ-irradiated polysaccharides	75:102	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes.
37123066	1	5	theme	Lentinula	327:335	arg1	LEP					345:347	LEP	345:347	LEP	345:347	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	5	theme	Lentinula	327:335	arg1	edodes					337:342	Lentinula edodes	327:342	Lentinula edodes (LEP)	327:348	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	6	from	influence	156:164	arg1	bioactivities					279:291	bioactivities	279:291	bioactivities	279:291	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	6	from	influence	156:164	arg1	composition					237:247	chemical composition	228:247	chemical composition	228:247	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	6	from	influence	156:164	arg1	features					266:273	physicochemical features	250:273	physicochemical features	250:273	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	6	7	theme	antioxidant	1100:1110	arg1	properties					1140:1149	its antioxidant, hypoglycemic and prebiotic properties	1096:1149	its antioxidant, hypoglycemic and prebiotic properties	1096:1149	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	4	8	theme	non-irradiated	853:866	arg1	LEP					868:870	non-irradiated LEP	853:870	non-irradiated LEP	853:870	γ-Irradiated LEP showed higher hypoglycemic activities in vitro than that of non-irradiated LEP.
37123066	2	9	theme	LEP	473:475	arg1	ability					462:468	DPPH scavenging ability	446:468	DPPH scavenging ability	446:468	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	9	theme	LEP	473:475	arg1	solubility					422:431	the solubility	418:431	the solubility	418:431	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	9	theme	LEP	473:475	arg1	⋅OH					438:440	the ⋅OH	434:440	the ⋅OH	434:440	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	9	theme	LEP	473:475	arg1	content					386:392	The carbohydrate content	369:392	The carbohydrate content (from 59.47 to 70.96%)	369:415	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	10	from	%	414:414	arg1	ability					462:468	DPPH scavenging ability	446:468	DPPH scavenging ability	446:468	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	10	from	%	414:414	arg1	solubility					422:431	the solubility	418:431	the solubility	418:431	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	10	from	%	414:414	arg1	⋅OH					438:440	the ⋅OH	434:440	the ⋅OH	434:440	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	10	from	%	414:414	arg1	content					386:392	The carbohydrate content	369:392	The carbohydrate content (from 59.47 to 70.96%)	369:415	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	11	theme	protein	536:542	arg1	content					544:550	the protein content	532:550	the protein content	532:550	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	5	12	dep	L.	974:975	arg1	plantarum					977:985	L. plantarum	974:985	L. plantarum	974:985	Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum.
37123066	7	13	from	10.1007/s10068-022-01234-5	1360:1385	arg1	available					1347:1355	available	1347:1355	available	1347:1355	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	6	14	dep	industries	1237:1246	arg1	the					1218:1220	the	1218:1220	the	1218:1220	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	5	15	dep	promoting	925:933	arg1	proliferation					911:923	proliferation	911:923	proliferation	911:923	Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum.
37123066	1	16	theme	chemical	228:235	arg1	composition					237:247	chemical composition	228:247	chemical composition	228:247	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	3	17	theme	γ-irradiation	693:705	arg1	treatment					707:715	γ-irradiation treatment	693:715	γ-irradiation treatment	693:715	Moreover, γ-irradiation treatment caused LEP color changes and surface topography destroyed.
37123066	2	18	theme	number-average	576:589	arg1	weight					601:606	number-average molecular weight	576:606	number-average molecular weight	576:606	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	19	theme	molecular	591:599	arg1	weight					601:606	number-average molecular weight	576:606	number-average molecular weight	576:606	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	6	20	theme	potential	1193:1201	arg1	application					1203:1213	potential application	1193:1213	potential application in the pharmaceutical industries and functional foods	1193:1267	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	1	21	theme	γ-irradiation	169:181	arg1	influence					156:164	the influence	152:164	the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP)	152:348	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	0	22	theme	Lentinula	119:127	arg1	edodes					129:134	Lentinula edodes	119:134	Lentinula edodes	119:134	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes.
37123066	0	23	theme	physicochemical	4:18	arg1	properties					61:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties	0:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides	0:102	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes.
37123066	6	24	dep	changes	1038:1044	arg1	affects					1088:1094	affects	1088:1094	affects	1088:1094	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	7	25	dep	Information	1284:1294	arg1	version					1307:1313	The online version	1296:1313	Supplementary Information The online version	1270:1313	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	6	26	from	application	1203:1213	arg1	industries					1237:1246	pharmaceutical industries	1222:1246	pharmaceutical industries	1222:1246	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	6	26	from	application	1203:1213	arg1	foods					1263:1267	functional foods	1252:1267	functional foods	1252:1267	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	1	27	theme	different	188:196	arg1	dose					198:201	different dose	188:201	different dose (0, 4, 8, and 16 kGy)	188:223	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	3	28	theme	LEP	724:726	arg1	changes					734:740	LEP color changes	724:740	LEP color changes	724:740	Moreover, γ-irradiation treatment caused LEP color changes and surface topography destroyed.
37123066	2	29	dep	70.96	409:413	arg1	to					406:407	to	406:407	to	406:407	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	0	30	theme	antioxidant	21:31	arg1	properties					61:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties	0:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides	0:102	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes.
37123066	3	31	theme	color	728:732	arg1	changes					734:740	LEP color changes	724:740	LEP color changes	724:740	Moreover, γ-irradiation treatment caused LEP color changes and surface topography destroyed.
37123066	2	32	theme	increased	496:504	arg1	dose					520:523	the increased γ-irradiation dose	492:523	the increased γ-irradiation dose	492:523	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	6	33	theme	functional	1252:1261	arg1	foods					1263:1267	functional foods	1252:1267	functional foods	1252:1267	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	2	34	theme	LEP	611:613	arg1	content					544:550	the protein content	532:550	the protein content	532:550	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	2	34	theme	LEP	611:613	arg1	weight					601:606	number-average molecular weight	576:606	number-average molecular weight	576:606	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	6	35	theme	LEP	1078:1080	arg1	features					1066:1073	the physicochemical features	1046:1073	the physicochemical features of LEP	1046:1080	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	6	36	theme	γ-irradiated	1172:1183	arg1	LEP					1185:1187	γ-irradiated LEP	1172:1187	γ-irradiated LEP	1172:1187	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	1	37	theme	physicochemical	250:264	arg1	features					266:273	physicochemical features	250:273	physicochemical features	250:273	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	6	38	dep	affects	1088:1094	arg1	suggests					1158:1165	suggests	1158:1165	suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods	1158:1267	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	7	39	theme	online	1300:1305	arg1	version					1307:1313	The online version	1296:1313	Supplementary Information The online version	1270:1313	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	0	40	theme	hypoglycemic	34:45	arg1	properties					61:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties	0:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides	0:102	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes.
37123066	4	41	theme	higher	800:805	arg1	activities					820:829	higher hypoglycemic activities	800:829	higher hypoglycemic activities	800:829	γ-Irradiated LEP showed higher hypoglycemic activities in vitro than that of non-irradiated LEP.
37123066	2	42	theme	carbohydrate	373:384	arg1	content					386:392	The carbohydrate content	369:392	The carbohydrate content (from 59.47 to 70.96%)	369:415	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	3	43	theme	surface	746:752	arg1	topography					754:763	surface topography	746:763	surface topography	746:763	Moreover, γ-irradiation treatment caused LEP color changes and surface topography destroyed.
37123066	1	44	dep	dose	198:201	arg1	0					204:204	0	204:204	0	204:204	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	44	dep	dose	198:201	arg1	8					210:210	8	210:210	8	210:210	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	44	dep	dose	198:201	arg1	16 kGy					217:222	16 kGy	217:222	16 kGy	217:222	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	44	dep	dose	198:201	arg1	4					207:207	4	207:207	4	207:207	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	7	45	theme	supplementary	1324:1336	arg1	material					1338:1345	supplementary material	1324:1345	supplementary material available at 10.1007/s10068-022-01234-5	1324:1385	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	2	46	theme	scavenging	451:460	arg1	ability					462:468	DPPH scavenging ability	446:468	DPPH scavenging ability	446:468	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	0	47	theme	prebiotic	51:59	arg1	properties					61:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties	0:70	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides	0:102	The physicochemical, antioxidant, hypoglycemic and prebiotic properties of γ-irradiated polysaccharides extracted from Lentinula edodes.
37123066	2	48	theme	γ-irradiation	663:675	arg1	dose					677:680	the increased γ-irradiation dose	649:680	the increased γ-irradiation dose	649:680	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	4	49	theme	hypoglycemic	807:818	arg1	activities					820:829	higher hypoglycemic activities	800:829	higher hypoglycemic activities	800:829	γ-Irradiated LEP showed higher hypoglycemic activities in vitro than that of non-irradiated LEP.
37123066	2	50	theme	increased	653:661	arg1	dose					677:680	the increased γ-irradiation dose	649:680	the increased γ-irradiation dose	649:680	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	7	51	theme	available	1347:1355	arg1	material					1338:1345	supplementary material	1324:1345	supplementary material available at 10.1007/s10068-022-01234-5	1324:1385	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	1	52	theme	polysaccharides	296:310	arg1	bioactivities					279:291	bioactivities	279:291	bioactivities	279:291	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	52	theme	polysaccharides	296:310	arg1	composition					237:247	chemical composition	228:247	chemical composition	228:247	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	1	52	theme	polysaccharides	296:310	arg1	features					266:273	physicochemical features	250:273	physicochemical features	250:273	In this study, the influence of γ-irradiation with different dose (0, 4, 8, and 16 kGy) on chemical composition, physicochemical features and bioactivities of polysaccharides extracted from Lentinula edodes (LEP) were investigated.
37123066	5	53	contain	had	900:902	arg2	effects					935:941	better proliferation promoting effects	904:941	better proliferation promoting effects	904:941	Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum.
37123066	5	53	contain	had	900:902	arg1	LEP					896:898	γ-irradiated LEP	883:898	γ-irradiated LEP	883:898	Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum.
37123066	6	54	theme	prebiotic	1130:1138	arg1	properties					1140:1149	its antioxidant, hypoglycemic and prebiotic properties	1096:1149	its antioxidant, hypoglycemic and prebiotic properties	1096:1149	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	6	55	contain	has	1189:1191	arg2	application					1203:1213	potential application	1193:1213	potential application in the pharmaceutical industries and functional foods	1193:1267	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	6	55	contain	has	1189:1191	arg1	LEP					1185:1187	γ-irradiated LEP	1172:1187	γ-irradiated LEP	1172:1187	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	2	56	theme	γ-irradiation	506:518	arg1	dose					520:523	the increased γ-irradiation dose	492:523	the increased γ-irradiation dose	492:523	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	5	57	theme	promoting	925:933	arg1	effects					935:941	better proliferation promoting effects	904:941	better proliferation promoting effects	904:941	Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum.
37123066	2	58	theme	DPPH	446:449	arg1	ability					462:468	DPPH scavenging ability	446:468	DPPH scavenging ability	446:468	The carbohydrate content (from 59.47 to 70.96%), the solubility, the ⋅OH and DPPH scavenging ability of LEP increased with the increased γ-irradiation dose, while the protein content, the weight-average and number-average molecular weight of LEP were significantly decreased with the increased γ-irradiation dose.
37123066	6	59	theme	physicochemical	1050:1064	arg1	features					1066:1073	the physicochemical features	1046:1073	the physicochemical features of LEP	1046:1080	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	6	60	theme	pharmaceutical	1222:1235	arg1	industries					1237:1246	pharmaceutical industries	1222:1246	pharmaceutical industries	1222:1246	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	5	61	dep	Lactobacillus	946:958	arg1	rhamnosus					960:968	Lactobacillus rhamnosus	946:968	Lactobacillus rhamnosus	946:968	Moreover, γ-irradiated LEP had better proliferation promoting effects on Lactobacillus rhamnosus and L. plantarum.
37123066	7	62	contain	contains	1315:1322	arg1	Information					1284:1294	Supplementary Information	1270:1294	Supplementary Information The online version	1270:1313	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	7	62	contain	contains	1315:1322	arg2	material					1338:1345	supplementary material	1324:1345	supplementary material available at 10.1007/s10068-022-01234-5	1324:1385	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	7	63	theme	Supplementary	1270:1282	arg1	Information					1284:1294	Supplementary Information	1270:1294	Supplementary Information The online version	1270:1313	Supplementary Information The online version contains supplementary material available at 10.1007/s10068-022-01234-5.
37123066	4	64	theme	γ-Irradiated	776:787	arg1	LEP					789:791	γ-Irradiated LEP	776:791	γ-Irradiated LEP	776:791	γ-Irradiated LEP showed higher hypoglycemic activities in vitro than that of non-irradiated LEP.
37123066	6	65	theme	hypoglycemic	1113:1124	arg1	properties					1140:1149	its antioxidant, hypoglycemic and prebiotic properties	1096:1149	its antioxidant, hypoglycemic and prebiotic properties	1096:1149	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
37123066	6	66	theme	γ-irradiation	1014:1026	arg1	treatment					1028:1036	γ-irradiation treatment	1014:1036	γ-irradiation treatment	1014:1036	These results showed that γ-irradiation treatment changes the physicochemical features of LEP, thus affects its antioxidant, hypoglycemic and prebiotic properties, which suggests that γ-irradiated LEP has potential application in the pharmaceutical industries and functional foods.
35893645	7	0	theme	Dazhuhuangbaima	1216:1230	arg1	hemicelluloses					1243:1256	'Dazhuhuangbaima' cell wall hemicelluloses	1215:1256	'Dazhuhuangbaima' cell wall hemicelluloses	1215:1256	Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38-73.10% and 50.05-64.45% Cd accumulating in the 'Zhongzhu 1' and 'Dazhuhuangbaima' cell wall hemicelluloses, respectively.
35893645	9	1	theme	≤	1600:1600	arg1	p					1598:1598	p ≤ 0.05	1598:1605	p ≤ 0.05	1598:1605	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	6	2	theme	ramie	1047:1051	arg1	populations					1053:1063	both ramie populations	1042:1063	both ramie populations	1042:1063	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	5	3	from	biomass	797:803	arg1	organs					831:836	all organs	827:836	all organs	827:836	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	6	4	theme	Cd	963:964	arg1	analysis					979:986	the subcellular Cd distribution analysis	947:986	the subcellular Cd distribution analysis	947:986	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	2	5	theme	components	208:217	arg1	capacity					182:189	the Cd binding capacity	167:189	the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species	167:268	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	5	6	dep	higher	857:862	arg1	≤					867:867	p ≤ 0.05	865:872	p ≤ 0.05	865:872	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	6	7	theme	cell	1029:1032	arg1	wall					1034:1037	the cell wall	1025:1037	the cell wall	1025:1037	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	2	8	theme	cell	198:201	arg1	components					208:217	the cell wall components	194:217	the cell wall components	194:217	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	5	9	from	enrichment	813:822	arg1	organs					831:836	all organs	827:836	all organs	827:836	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	9	10	from	concentration	1530:1542	arg1	populations					1552:1562	both populations	1547:1562	both populations	1547:1562	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	4	11	theme	cell	696:699	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	12	theme	hemicellulose	727:739	arg1	content					741:747	hemicellulose content	727:747	hemicellulose content	727:747	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	2	13	theme	wall	232:235	arg1	compositions					237:248	the cell wall compositions	223:248	the cell wall compositions	223:248	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	9	14	theme	Hemicellulose	1423:1435	arg1	analysis					1445:1452	Hemicellulose content analysis	1423:1452	Hemicellulose content analysis	1423:1452	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	4	15	theme	root	584:587	arg1	length					589:594	their root length	578:594	their root length	578:594	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	5	16	theme	p	865:865	arg1	≤					867:867	p ≤ 0.05	865:872	p ≤ 0.05	865:872	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	4	17	from	distribution	664:675	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	17	from	distribution	664:675	arg1	organs					641:646	the organs	637:646	the organs	637:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	18	from	content	741:747	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	18	from	content	741:747	arg1	organs					641:646	the organs	637:646	the organs	637:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	1	19	theme	cell	92:95	arg1	walls					97:101	Ramie cell walls	86:101	Ramie cell walls	86:101	Ramie cell walls play an important role in cadmium (Cd) detoxification.
35893645	1	20	theme	Ramie	86:90	arg1	walls					97:101	Ramie cell walls	86:101	Ramie cell walls	86:101	Ramie cell walls play an important role in cadmium (Cd) detoxification.
35893645	2	21	theme	binding	174:180	arg1	capacity					182:189	the Cd binding capacity	167:189	the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species	167:268	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	7	22	dep	%	1156:1156	arg1	Cd					1175:1176	Cd	1175:1176	Cd	1175:1176	Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38-73.10% and 50.05-64.45% Cd accumulating in the 'Zhongzhu 1' and 'Dazhuhuangbaima' cell wall hemicelluloses, respectively.
35893645	8	23	dep	higher	1355:1360	arg1	p					1363:1363	p ≤ 0.05	1363:1370	p ≤ 0.05	1363:1370	However, the Cd concentration in the 'Zhongzhu 1' hemicellulose was significantly higher (p ≤ 0.05) than that in the 'Dazhuhuangbaima' hemicellulose.
35893645	9	24	dep	higher	1590:1595	arg1	p					1598:1598	p ≤ 0.05	1598:1605	p ≤ 0.05	1598:1605	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	1	25	theme	important	111:119	arg1	role					121:124	an important role	108:124	an important role	108:124	Ramie cell walls play an important role in cadmium (Cd) detoxification.
35893645	4	26	theme	Cd	620:621	arg1	enrichment					623:632	Cd enrichment	620:632	Cd enrichment in the organs	620:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	12	27	from	enrichment	1911:1920	arg1	ramie					1934:1938	ramie	1934:1938	ramie	1934:1938	It also provides a theoretical basis for Cd enrichment breeding in ramie.
35893645	3	28	theme	enrichment	462:471	arg1	characteristics					473:487	different Cd enrichment characteristics	449:487	different Cd enrichment characteristics	449:487	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	4	29	from	biomass	611:617	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	29	from	biomass	611:617	arg1	organs					641:646	the organs	637:646	the organs	637:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	5	30	theme	Cd	810:811	arg1	enrichment					813:822	Cd enrichment	810:822	Cd enrichment in all organs	810:836	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	9	31	theme	Cd	1527:1528	arg1	concentration					1530:1542	the Cd concentration	1523:1542	the Cd concentration in both populations	1523:1562	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	4	32	from	height	603:608	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	32	from	height	603:608	arg1	organs					641:646	the organs	637:646	the organs	637:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	5	33	theme	plant	783:787	arg1	height					789:794	plant height	783:794	plant height	783:794	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	0	34	dep	Ramie	50:54	arg1	Boehmeria					57:65	Boehmeria	57:65	Boehmeria	57:65	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)
35893645	0	34	dep	Ramie	50:54	arg1	L.					74:75	L.	74:75	L.	74:75	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)
35893645	0	34	dep	Ramie	50:54	arg1	Gaud					78:81	Gaud	78:81	Gaud	78:81	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)
35893645	7	35	theme	cell	1076:1079	arg1	fractions					1086:1094	the cell wall fractions	1072:1094	the cell wall fractions	1072:1094	Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38-73.10% and 50.05-64.45% Cd accumulating in the 'Zhongzhu 1' and 'Dazhuhuangbaima' cell wall hemicelluloses, respectively.
35893645	8	36	theme	Cd	1286:1287	arg1	higher					1355:1360	higher	1355:1360	higher	1355:1360	However, the Cd concentration in the 'Zhongzhu 1' hemicellulose was significantly higher (p ≤ 0.05) than that in the 'Dazhuhuangbaima' hemicellulose.
35893645	8	36	theme	Cd	1286:1287	arg1	concentration					1289:1301	the Cd concentration	1282:1301	the Cd concentration in the 'Zhongzhu 1' hemicellulose	1282:1335	However, the Cd concentration in the 'Zhongzhu 1' hemicellulose was significantly higher (p ≤ 0.05) than that in the 'Dazhuhuangbaima' hemicellulose.
35893645	3	37	theme	ramie	322:326	arg1	populations					328:338	two ramie populations	318:338	two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics	318:487	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	37	theme	ramie	322:326	arg1	Zhongzhu					397:404	Zhongzhu	397:404	Zhongzhu	397:404	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	37	theme	ramie	322:326	arg1	Dazhuhuangbaima					342:356	Dazhuhuangbaima	342:356	Dazhuhuangbaima	342:356	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	6	38	located	found	1016:1020	arg1	wall					1034:1037	the cell wall	1025:1037	the cell wall	1025:1037	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	6	38	located	found	1016:1020	arg2	Cd					1002:1003	Cd	1002:1003	Cd	1002:1003	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	6	38	located	found	1016:1020	arg1	populations					1053:1063	both ramie populations	1042:1063	both ramie populations	1042:1063	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	4	39	theme	Cd	678:679	arg1	content					681:687	Cd content	678:687	Cd content in the cell wall polysaccharides	678:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	7	40	theme	wall	1238:1241	arg1	hemicelluloses					1243:1256	'Dazhuhuangbaima' cell wall hemicelluloses	1215:1256	'Dazhuhuangbaima' cell wall hemicelluloses	1215:1256	Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38-73.10% and 50.05-64.45% Cd accumulating in the 'Zhongzhu 1' and 'Dazhuhuangbaima' cell wall hemicelluloses, respectively.
35893645	2	41	theme	ramie	256:260	arg1	species					262:268	ramie species	256:268	ramie species	256:268	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	8	42	theme	≤	1365:1365	arg1	p					1363:1363	p ≤ 0.05	1363:1370	p ≤ 0.05	1363:1370	However, the Cd concentration in the 'Zhongzhu 1' hemicellulose was significantly higher (p ≤ 0.05) than that in the 'Dazhuhuangbaima' hemicellulose.
35893645	3	43	theme	different	449:457	arg1	characteristics					473:487	different Cd enrichment characteristics	449:487	different Cd enrichment characteristics	449:487	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	10	44	theme	hemicellulose	1728:1740	arg1	content					1742:1748	the hemicellulose content	1724:1748	the hemicellulose content in the cell wall	1724:1765	Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall.
35893645	4	45	theme	subcellular	649:659	arg1	distribution					664:675	subcellular Cd distribution	649:675	subcellular Cd distribution	649:675	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	8	46	from	concentration	1289:1301	arg1	hemicellulose					1323:1335	the 'Zhongzhu 1' hemicellulose	1306:1335	the 'Zhongzhu 1' hemicellulose	1306:1335	However, the Cd concentration in the 'Zhongzhu 1' hemicellulose was significantly higher (p ≤ 0.05) than that in the 'Dazhuhuangbaima' hemicellulose.
35893645	3	47	theme	high-Cd-accumulating	410:429	arg1	population					431:440	high-Cd-accumulating population	410:440	high-Cd-accumulating population	410:440	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	47	theme	high-Cd-accumulating	410:429	arg1	Zhongzhu					397:404	Zhongzhu	397:404	Zhongzhu	397:404	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	4	48	theme	wall	701:704	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	10	49	theme	cell	1757:1760	arg1	wall					1762:1765	the cell wall	1753:1765	the cell wall	1753:1765	Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall.
35893645	4	50	theme	ramie	498:502	arg1	populations					504:514	The two ramie populations	490:514	The two ramie populations	490:514	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	3	51	theme	Cd	459:460	arg1	characteristics					473:487	different Cd enrichment characteristics	449:487	different Cd enrichment characteristics	449:487	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	52	with	populations	328:338	arg1	characteristics					473:487	different Cd enrichment characteristics	449:487	different Cd enrichment characteristics	449:487	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	2	53	theme	wall	203:206	arg1	components					208:217	the cell wall components	194:217	the cell wall components	194:217	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	8	54	theme	Dazhuhuangbaima	1391:1405	arg1	hemicellulose					1408:1420	the 'Dazhuhuangbaima' hemicellulose	1386:1420	the 'Dazhuhuangbaima' hemicellulose	1386:1420	However, the Cd concentration in the 'Zhongzhu 1' hemicellulose was significantly higher (p ≤ 0.05) than that in the 'Dazhuhuangbaima' hemicellulose.
35893645	4	55	theme	kg-1	551:554	arg1	Cd					556:557	0, 25, and 75 mg kg-1 Cd	534:557	0, 25, and 75 mg kg-1 Cd	534:557	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	2	56	theme	compositions	237:248	arg1	capacity					182:189	the Cd binding capacity	167:189	the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species	167:268	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	1	57	theme	cadmium	129:135	arg1	detoxification					142:155	cadmium (Cd) detoxification	129:155	cadmium (Cd) detoxification	129:155	Ramie cell walls play an important role in cadmium (Cd) detoxification.
35893645	2	58	theme	cell	227:230	arg1	compositions					237:248	the cell wall compositions	223:248	the cell wall compositions	223:248	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	2	59	theme	Cd	171:172	arg1	capacity					182:189	the Cd binding capacity	167:189	the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species	167:268	However, the Cd binding capacity of the cell wall components and the cell wall compositions among ramie species remains unclear.
35893645	11	60	from	findings	1772:1779	arg1	study					1789:1793	this study	1784:1793	this study	1784:1793	The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie.
35893645	6	61	theme	subcellular	951:961	arg1	analysis					979:986	the subcellular Cd distribution analysis	947:986	the subcellular Cd distribution analysis	947:986	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	9	62	theme	content	1437:1443	arg1	analysis					1445:1452	Hemicellulose content analysis	1423:1452	Hemicellulose content analysis	1423:1452	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	5	63	theme	root	770:773	arg1	higher					857:862	higher	857:862	higher	857:862	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	5	63	theme	root	770:773	arg1	length					775:780	The root length	766:780	The root length	766:780	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	9	64	theme	Cd	1661:1662	arg1	treatments					1664:1673	all Cd treatments	1657:1673	all Cd treatments	1657:1673	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	4	65	from	length	589:594	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	65	from	length	589:594	arg1	organs					641:646	the organs	637:646	the organs	637:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	6	66	theme	distribution	966:977	arg1	analysis					979:986	the subcellular Cd distribution analysis	947:986	the subcellular Cd distribution analysis	947:986	In addition, the subcellular Cd distribution analysis revealed that Cd was mainly found in the cell wall in both ramie populations.
35893645	9	67	theme	hemicellulose	1480:1492	arg1	concentration					1494:1506	the hemicellulose concentration	1476:1506	the hemicellulose concentration	1476:1506	Hemicellulose content analysis further revealed that the hemicellulose concentration increased with the Cd concentration in both populations, but it was significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' across all Cd treatments.
35893645	0	68	from	Role	4:7	arg1	Ramie					50:54	Ramie	50:54	Ramie	50:54	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)
35893645	0	68	from	Role	4:7	arg1	Tolerance					37:45	Cadmium Tolerance	29:45	Cadmium Tolerance	29:45	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)
35893645	5	69	theme	Cd	923:924	arg1	stress					926:931	Cd stress	923:931	Cd stress	923:931	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	4	70	from	enrichment	623:632	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	70	from	enrichment	623:632	arg1	organs					641:646	the organs	637:646	the organs	637:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	12	71	theme	Cd	1908:1909	arg1	enrichment					1911:1920	Cd enrichment breeding	1908:1929	Cd enrichment breeding in ramie	1908:1938	It also provides a theoretical basis for Cd enrichment breeding in ramie.
35893645	10	72	theme	Cd	1700:1701	arg1	stress					1703:1708	Cd stress	1700:1708	Cd stress	1700:1708	Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall.
35893645	12	73	theme	breeding	1922:1929	arg1	enrichment					1911:1920	Cd enrichment breeding	1908:1929	Cd enrichment breeding in ramie	1908:1938	It also provides a theoretical basis for Cd enrichment breeding in ramie.
35893645	3	74	dep	populations	328:338	arg1	population					431:440	high-Cd-accumulating population	410:440	high-Cd-accumulating population	410:440	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	74	dep	populations	328:338	arg1	Zhongzhu					397:404	Zhongzhu	397:404	Zhongzhu	397:404	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	74	dep	populations	328:338	arg1	Dazhuhuangbaima					342:356	Dazhuhuangbaima	342:356	Dazhuhuangbaima	342:356	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	74	dep	populations	328:338	arg1	population					380:389	low-Cd-accumulating population	360:389	low-Cd-accumulating population	360:389	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	74	dep	populations	328:338	arg1	populations					328:338	two ramie populations	318:338	two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics	318:487	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	10	75	from	content	1742:1748	arg1	wall					1762:1765	the cell wall	1753:1765	the cell wall	1753:1765	Thus, ramie copes under Cd stress by increasing the hemicellulose content in the cell wall.
35893645	3	76	theme	low-Cd-accumulating	360:378	arg1	Dazhuhuangbaima					342:356	Dazhuhuangbaima	342:356	Dazhuhuangbaima	342:356	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	3	76	theme	low-Cd-accumulating	360:378	arg1	population					380:389	low-Cd-accumulating population	360:389	low-Cd-accumulating population	360:389	Therefore, this study compared two ramie populations ('Dazhuhuangbaima' (low-Cd-accumulating population) and 'Zhongzhu 1' (high-Cd-accumulating population)) with different Cd enrichment characteristics.
35893645	4	77	from	content	681:687	arg1	polysaccharides					706:720	the cell wall polysaccharides	692:720	the cell wall polysaccharides	692:720	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	77	from	content	681:687	arg1	organs					641:646	the organs	637:646	the organs	637:646	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	5	78	from	length	775:780	arg1	organs					831:836	all organs	827:836	all organs	827:836	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	7	79	theme	wall	1081:1084	arg1	fractions					1086:1094	the cell wall fractions	1072:1094	the cell wall fractions	1072:1094	Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38-73.10% and 50.05-64.45% Cd accumulating in the 'Zhongzhu 1' and 'Dazhuhuangbaima' cell wall hemicelluloses, respectively.
35893645	11	80	theme	enrichment	1834:1843	arg1	site					1845:1848	the main enrichment site	1825:1848	the main enrichment site for Cd in ramie	1825:1864	The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie.
35893645	11	80	theme	enrichment	1834:1843	arg1	hemicellulose					1808:1820	hemicellulose	1808:1820	hemicellulose	1808:1820	The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie.
35893645	4	81	theme	plant	597:601	arg1	height					603:608	plant height	597:608	plant height	597:608	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	0	82	theme	Hemicellulose	12:24	arg1	Role					4:7	The Role	0:7	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)	0:83	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)
35893645	0	83	theme	Cadmium	29:35	arg1	Tolerance					37:45	Cadmium Tolerance	29:45	Cadmium Tolerance	29:45	The Role of Hemicellulose in Cadmium Tolerance in Ramie (Boehmeria nivea (L.) Gaud.)
35893645	4	84	dep	mg	548:549	arg1	75					545:546	75	545:546	75	545:546	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	84	dep	mg	548:549	arg1	25					537:538	25	537:538	25	537:538	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	4	85	theme	Cd	661:662	arg1	distribution					664:675	subcellular Cd distribution	649:675	subcellular Cd distribution	649:675	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	8	86	theme	Zhongzhu	1311:1318	arg1	hemicellulose					1323:1335	the 'Zhongzhu 1' hemicellulose	1306:1335	the 'Zhongzhu 1' hemicellulose	1306:1335	However, the Cd concentration in the 'Zhongzhu 1' hemicellulose was significantly higher (p ≤ 0.05) than that in the 'Dazhuhuangbaima' hemicellulose.
35893645	5	87	from	height	789:794	arg1	organs					831:836	all organs	827:836	all organs	827:836	The root length, plant height, biomass, and Cd enrichment in all organs were significantly higher (p ≤ 0.05) in 'Zhongzhu 1' than in 'Dazhuhuangbaima' under Cd stress.
35893645	12	88	theme	theoretical	1886:1896	arg1	basis					1898:1902	a theoretical basis	1884:1902	a theoretical basis for Cd enrichment breeding in ramie	1884:1938	It also provides a theoretical basis for Cd enrichment breeding in ramie.
35893645	7	89	theme	cell	1233:1236	arg1	hemicelluloses					1243:1256	'Dazhuhuangbaima' cell wall hemicelluloses	1215:1256	'Dazhuhuangbaima' cell wall hemicelluloses	1215:1256	Among the cell wall fractions, Cd was mainly bound to the hemicelluloses, with 60.38-73.10% and 50.05-64.45% Cd accumulating in the 'Zhongzhu 1' and 'Dazhuhuangbaima' cell wall hemicelluloses, respectively.
35893645	4	90	dep	Cd	556:557	arg1	mg					548:549	mg	548:549	mg	548:549	The two ramie populations were treated with 0, 25, and 75 mg kg-1 Cd for 30 days; then, their root length, plant height, biomass, Cd enrichment in the organs, subcellular Cd distribution, Cd content in the cell wall polysaccharides, and hemicellulose content were determined.
35893645	11	91	from	site	1845:1848	arg1	ramie					1860:1864	ramie	1860:1864	ramie	1860:1864	The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie.
35893645	11	92	theme	main	1829:1832	arg1	site					1845:1848	the main enrichment site	1825:1848	the main enrichment site for Cd in ramie	1825:1864	The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie.
35893645	11	92	theme	main	1829:1832	arg1	hemicellulose					1808:1820	hemicellulose	1808:1820	hemicellulose	1808:1820	The findings in this study confirm that hemicellulose is the main enrichment site for Cd in ramie.
35744381	0	0	theme	Methods	88:94	arg1	Comparison					60:69	Ammonium Alginate-The Comparison	38:69	Ammonium Alginate-The Comparison of Two Synthesis Methods	38:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	3	1	from	studies	436:442	arg1	properties					451:460	the properties	447:460	the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution	447:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	0	2	theme	Synthesis	78:86	arg1	Methods					88:94	Two Synthesis Methods	74:94	Two Synthesis Methods	74:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	2	3	theme	groups	343:348	arg1	substitution					322:333	a full substitution	315:333	a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid	315:407	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	5	4	theme	weight	984:989	arg1	electrolyte					991:1001	a low molecular weight electrolyte	968:1001	a low molecular weight electrolyte	968:1001	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	3	5	with	reaction	516:523	arg1	those					530:534	those	530:534	those	530:534	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	2	6	theme	ATR	284:286	arg1	analysis					301:308	FTIR ATR spectroscopy analysis	279:308	FTIR ATR spectroscopy analysis	279:308	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	2	7	theme	FTIR	279:282	arg1	analysis					301:308	FTIR ATR spectroscopy analysis	279:308	FTIR ATR spectroscopy analysis	279:308	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	2	8	theme	full	317:320	arg1	substitution					322:333	a full substitution	315:333	a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid	315:407	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	3	9	from	properties	647:656	arg1	derivatives					690:700	these two derivatives	680:700	these two derivatives	680:700	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	10	theme	solutions	483:491	arg1	properties					451:460	the properties	447:460	the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution	447:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	11	from	solution	610:617	arg1	method					562:567	the classical method	548:567	the classical method of dissolving alginic acid in an ammonia solution	548:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	12	theme	Comparative	424:434	arg1	studies					436:442	Comparative studies	424:442	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution	424:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	5	13	theme	low	970:972	arg1	weight					984:989	a low molecular weight	968:989	a low molecular weight electrolyte	968:1001	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	0	14	theme	Interphase	4:13	arg1	Synthesis					25:33	The Interphase Gas-Solid Synthesis	0:33	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods	0:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	5	15	from	decrease	1036:1043	arg1	viscosity					1048:1056	viscosity	1048:1056	viscosity	1048:1056	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	4	16	theme	alginate	849:856	arg1	solutions					829:837	solutions	829:837	solutions of sodium alginate	829:856	Moreover, it was shown that aqueous solutions of ammonium alginate are more stable over time than solutions of sodium alginate.
35744381	3	17	theme	solutions	665:673	arg1	properties					647:656	the rheological properties	631:656	the rheological properties of the solutions from these two derivatives	631:700	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	2	18	from	groups	362:367	arg1	surface					376:382	the surface	372:382	the surface of powdered alginic acid	372:407	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	3	19	theme	dissolving	572:581	arg1	acid					591:594	dissolving alginic acid	572:594	dissolving alginic acid in an ammonia solution	572:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	4	20	theme	aqueous	759:765	arg1	solutions					767:775	aqueous solutions	759:775	aqueous solutions of ammonium alginate	759:796	Moreover, it was shown that aqueous solutions of ammonium alginate are more stable over time than solutions of sodium alginate.
35744381	5	21	theme	electrolyte	991:1001	arg1	addition					956:963	the addition	952:963	the addition of a low molecular weight electrolyte to their solutions	952:1020	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	0	22	theme	Molecular	118:126	arg1	Weight					128:133	Low Molecular Weight	114:133	Low Molecular Weight Electrolyte Presence	114:154	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	5	23	theme	typical	923:929	arg1	alginate					890:897	ammonium alginate	881:897	ammonium alginate	881:897	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	5	23	theme	typical	923:929	arg1	alginate					910:917	sodium alginate	903:917	sodium alginate	903:917	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	5	23	theme	typical	923:929	arg1	polyelectrolytes					931:946	typical polyelectrolytes	923:946	typical polyelectrolytes	923:946	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	3	24	theme	classical	552:560	arg1	method					562:567	the classical method	548:567	the classical method of dissolving alginic acid in an ammonia solution	548:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	25	from	acid	591:594	arg1	solution					610:617	an ammonia solution	599:617	an ammonia solution	599:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	0	26	theme	Gas-Solid	15:23	arg1	Synthesis					25:33	The Interphase Gas-Solid Synthesis	0:33	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods	0:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	0	27	theme	Low	114:116	arg1	Weight					128:133	Low Molecular Weight	114:133	Low Molecular Weight Electrolyte Presence	114:154	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	2	28	theme	spectroscopy	288:299	arg1	analysis					301:308	FTIR ATR spectroscopy analysis	279:308	FTIR ATR spectroscopy analysis	279:308	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	2	29	theme	acid	404:407	arg1	surface					376:382	the surface	372:382	the surface of powdered alginic acid	372:407	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	0	30	theme	Ammonium	38:45	arg1	Comparison					60:69	Ammonium Alginate-The Comparison	38:69	Ammonium Alginate-The Comparison of Two Synthesis Methods	38:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	0	31	theme	Electrolyte	135:145	arg1	Presence					147:154	Electrolyte Presence	135:154	Low Molecular Weight Electrolyte Presence	114:154	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	2	32	theme	alginic	396:402	arg1	acid					404:407	powdered alginic acid	387:407	powdered alginic acid	387:407	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	3	33	from	derivatives	690:700	arg1	properties					647:656	the rheological properties	631:656	the rheological properties of the solutions from these two derivatives	631:700	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	33	from	derivatives	690:700	arg1	solutions					665:673	the solutions	661:673	the solutions from these two derivatives	661:700	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	0	34	theme	Weight	128:133	arg1	Synthesis					25:33	The Interphase Gas-Solid Synthesis	0:33	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods	0:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	0	34	theme	Weight	128:133	arg1	Effect					104:109	the Effect	100:109	the Effect of Low Molecular Weight Electrolyte Presence	100:154	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	1	35	theme	ammonium	207:214	arg1	alginate					216:223	ammonium alginate	207:223	ammonium alginate	207:223	This paper presents a method for the synthesis of ammonium alginate by interphase gas-solid reaction.
35744381	0	36	theme	Comparison	60:69	arg1	Synthesis					25:33	The Interphase Gas-Solid Synthesis	0:33	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods	0:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	0	36	theme	Comparison	60:69	arg1	Effect					104:109	the Effect	100:109	the Effect of Low Molecular Weight Electrolyte Presence	100:154	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	3	37	theme	rheological	635:645	arg1	properties					647:656	the rheological properties	631:656	the rheological properties of the solutions from these two derivatives	631:700	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	38	theme	alginic	583:589	arg1	acid					591:594	dissolving alginic acid	572:594	dissolving alginic acid in an ammonia solution	572:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	4	39	theme	sodium	842:847	arg1	alginate					849:856	sodium alginate	842:856	sodium alginate	842:856	Moreover, it was shown that aqueous solutions of ammonium alginate are more stable over time than solutions of sodium alginate.
35744381	2	40	theme	acid	338:341	arg1	groups					343:348	acid groups	338:348	acid groups	338:348	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	0	41	theme	Alginate-The	47:58	arg1	Comparison					60:69	Ammonium Alginate-The Comparison	38:69	Ammonium Alginate-The Comparison of Two Synthesis Methods	38:94	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	3	42	theme	acid	591:594	arg1	method					562:567	the classical method	548:567	the classical method of dissolving alginic acid in an ammonia solution	548:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	43	theme	interphase	505:514	arg1	reaction					516:523	interphase reaction	505:523	interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution	505:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	5	44	theme	molecular	974:982	arg1	weight					984:989	a low molecular weight	968:989	a low molecular weight electrolyte	968:1001	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	4	45	theme	alginate	789:796	arg1	solutions					767:775	aqueous solutions	759:775	aqueous solutions of ammonium alginate	759:796	Moreover, it was shown that aqueous solutions of ammonium alginate are more stable over time than solutions of sodium alginate.
35744381	3	46	theme	ammonium	465:472	arg1	solutions					483:491	ammonium alginate solutions	465:491	ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution	465:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	3	47	from	method	562:567	arg1	solution					610:617	an ammonia solution	599:617	an ammonia solution	599:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	1	48	theme	alginate	216:223	arg1	synthesis					194:202	the synthesis	190:202	the synthesis of ammonium alginate by interphase gas-solid reaction	190:256	This paper presents a method for the synthesis of ammonium alginate by interphase gas-solid reaction.
35744381	5	49	theme	sodium	903:908	arg1	alginate					890:897	ammonium alginate	881:897	ammonium alginate	881:897	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	5	49	theme	sodium	903:908	arg1	alginate					910:917	sodium alginate	903:917	sodium alginate	903:917	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	5	49	theme	sodium	903:908	arg1	polyelectrolytes					931:946	typical polyelectrolytes	923:946	typical polyelectrolytes	923:946	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	4	50	theme	ammonium	780:787	arg1	alginate					789:796	ammonium alginate	780:796	ammonium alginate	780:796	Moreover, it was shown that aqueous solutions of ammonium alginate are more stable over time than solutions of sodium alginate.
35744381	0	51	dep	Weight	128:133	arg1	Presence					147:154	Electrolyte Presence	135:154	Low Molecular Weight Electrolyte Presence	114:154	The Interphase Gas-Solid Synthesis of Ammonium Alginate-The Comparison of Two Synthesis Methods and the Effect of Low Molecular Weight Electrolyte Presence.
35744381	2	52	theme	powdered	387:394	arg1	acid					404:407	powdered alginic acid	387:407	powdered alginic acid	387:407	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	3	53	theme	ammonia	602:608	arg1	solution					610:617	an ammonia solution	599:617	an ammonia solution	599:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	5	54	theme	ammonium	881:888	arg1	alginate					890:897	ammonium alginate	881:897	ammonium alginate	881:897	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	5	54	theme	ammonium	881:888	arg1	alginate					910:917	sodium alginate	903:917	sodium alginate	903:917	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	5	54	theme	ammonium	881:888	arg1	polyelectrolytes					931:946	typical polyelectrolytes	923:946	typical polyelectrolytes	923:946	It was confirmed that ammonium alginate and sodium alginate are typical polyelectrolytes, as the addition of a low molecular weight electrolyte to their solutions resulted in a decrease in viscosity.
35744381	3	55	theme	alginate	474:481	arg1	solutions					483:491	ammonium alginate solutions	465:491	ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution	465:617	Comparative studies on the properties of ammonium alginate solutions obtained by interphase reaction with those prepared by the classical method of dissolving alginic acid in an ammonia solution showed that the rheological properties of the solutions from these two derivatives do not differ significantly.
35744381	1	56	theme	interphase	228:237	arg1	reaction					249:256	interphase gas-solid reaction	228:256	interphase gas-solid reaction	228:256	This paper presents a method for the synthesis of ammonium alginate by interphase gas-solid reaction.
35744381	2	57	theme	ammonium	353:360	arg1	groups					362:367	ammonium groups	353:367	ammonium groups on the surface of powdered alginic acid	353:407	It was confirmed by FTIR ATR spectroscopy analysis that a full substitution of acid groups by ammonium groups on the surface of powdered alginic acid was performed.
35744381	1	58	theme	gas-solid	239:247	arg1	reaction					249:256	interphase gas-solid reaction	228:256	interphase gas-solid reaction	228:256	This paper presents a method for the synthesis of ammonium alginate by interphase gas-solid reaction.
36091763	6	0	from	roots	540:544	arg1	poor					560:563	poor	560:563	poor	560:563	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	0	from	roots	540:544	arg1	activities					480:489	the antioxidant activities	464:489	the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1)	464:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	0	from	roots	540:544	arg1	polysaccharides					498:512	the polysaccharides	494:512	the polysaccharides from rhizomes and fibrous roots (CLFP-1)	494:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	3	1	theme	different	193:201	arg1	parts					209:213	different plant parts	193:213	different plant parts of Codonopsis pilosula var	193:240	In this study, three acidic polysaccharides from different plant parts of Codonopsis pilosula var.
36091763	12	2	theme	cell	1503:1506	arg1	viability					1508:1516	cell viability	1503:1516	cell viability	1503:1516	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	10	3	theme	long	1307:1310	arg1	regions					1329:1335	long homogalacturonan regions	1307:1335	long homogalacturonan regions	1307:1335	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	13	4	theme	reactive	1943:1950	arg1	species					1959:1965	reactive oxygen species	1943:1965	reactive oxygen species	1943:1965	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	3	5	theme	var	238:240	arg1	parts					209:213	different plant parts	193:213	different plant parts of Codonopsis pilosula var	193:240	In this study, three acidic polysaccharides from different plant parts of Codonopsis pilosula var.
36091763	11	6	theme	side	1407:1410	arg1	chains					1412:1417	side chains	1407:1417	side chains	1407:1417	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	11	6	theme	side	1407:1410	arg1	type					1354:1357	Arabinogalactan type I	1338:1359	Arabinogalactan type I	1338:1359	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	11	6	theme	side	1407:1410	arg1	type					1381:1384	arabinogalactan type II	1365:1387	arabinogalactan type II	1365:1387	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	7	7	theme	antioxidant	779:789	arg1	activities					791:800	their antioxidant activities	773:800	their antioxidant activities	773:800	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	9	8	from	GalA	1094:1097	arg1	ratio					1020:1024	a ratio	1018:1024	a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7	1018:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	8	from	GalA	1094:1097	arg1	ratio					1104:1108	a ratio	1102:1108	a ratio of 5.8: 8.9: 8.0: 77.0	1102:1131	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	10	9	with	acid	1297:1300	arg1	regions					1329:1335	long homogalacturonan regions	1307:1335	long homogalacturonan regions	1307:1335	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	13	10	theme	antioxidant	1891:1901	arg1	activities					1911:1920	antioxidant enzymes activities	1891:1920	antioxidant enzymes activities	1891:1920	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	9	11	from	Rha	1084:1086	arg1	ratio					1020:1024	a ratio	1018:1024	a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7	1018:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	11	from	Rha	1084:1086	arg1	ratio					1104:1108	a ratio	1102:1108	a ratio of 5.8: 8.9: 8.0: 77.0	1102:1131	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	6	12	from	activities	480:489	arg1	CLFP-1					547:552	CLFP-1	547:552	CLFP-1	547:552	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	12	from	activities	480:489	arg1	rhizomes					519:526	rhizomes	519:526	rhizomes	519:526	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	12	from	activities	480:489	arg1	roots					540:544	fibrous roots	532:544	fibrous roots (CLFP-1)	532:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	9	13	theme	monosaccharide	933:946	arg1	Ara					974:976	Ara	974:976	Ara	974:976	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	13	theme	monosaccharide	933:946	arg1	composition					948:958	The monosaccharide composition	929:958	The monosaccharide composition of CLRP-1	929:968	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	13	14	theme	transcription	1861:1873	arg1	factor					1875:1880	DAF-16 transcription factor	1854:1880	DAF-16 transcription factor	1854:1880	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	7	15	theme	polysaccharides	639:653	arg1	features					627:634	the structural features	612:634	the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1)	612:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	15	theme	polysaccharides	639:653	arg1	object					710:715	the object	706:715	the object for this study	706:730	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	5	16	theme	ion	288:290	arg1	chromatography					301:314	ion exchange chromatography	288:314	ion exchange chromatography	288:314	L. T. Shen were obtained by ion exchange chromatography and gel filtration chromatography, and the yields of these three polysaccharides were different.
36091763	7	17	theme	structural	616:625	arg1	features					627:634	the structural features	612:634	the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1)	612:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	17	theme	structural	616:625	arg1	object					710:715	the object	706:715	the object for this study	706:730	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	12	18	from	assay	1436:1440	arg1	cells					1453:1457	IPEC-J2 cells	1445:1457	IPEC-J2 cells	1445:1457	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	0	19	theme	different	71:79	arg1	parts					81:85	different parts	71:85	different parts of Codonopsis pilosula var	71:112	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	13	20	theme	nuclear	1830:1836	arg1	localization					1838:1849	the nuclear localization	1826:1849	the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities	1826:1920	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	14	21	used	used	2119:2122	arg2	CLRP-1					2092:2097	CLRP-1	2092:2097	CLRP-1	2092:2097	Thus, these finding suggest that CLRP-1 and CLSP-1 could be used as potential antioxidants.
36091763	14	21	used	used	2119:2122	arg2	antioxidants					2137:2148	potential antioxidants	2127:2148	potential antioxidants	2127:2148	Thus, these finding suggest that CLRP-1 and CLSP-1 could be used as potential antioxidants.
36091763	14	21	used	used	2119:2122	arg2	CLSP-1					2103:2108	CLSP-1	2103:2108	CLSP-1	2103:2108	Thus, these finding suggest that CLRP-1 and CLSP-1 could be used as potential antioxidants.
36091763	12	22	theme	IPEC-J2	1445:1451	arg1	cells					1453:1457	IPEC-J2 cells	1445:1457	IPEC-J2 cells	1445:1457	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	10	23	theme	structural	1163:1172	arg1	elucidation					1174:1184	structural elucidation	1163:1184	structural elucidation	1163:1184	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	9	24	from	Fuc	984:986	arg1	ratio					1020:1024	a ratio	1018:1024	a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7	1018:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	24	from	Fuc	984:986	arg1	ratio					1104:1108	a ratio	1102:1108	a ratio of 5.8: 8.9: 8.0: 77.0	1102:1131	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	25	from	Rha	979:981	arg1	ratio					1020:1024	a ratio	1018:1024	a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7	1018:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	25	from	Rha	979:981	arg1	ratio					1104:1108	a ratio	1102:1108	a ratio of 5.8: 8.9: 8.0: 77.0	1102:1131	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	12	26	theme	dismutase	1644:1652	arg1	activity					1621:1628	the activity	1617:1628	the activity of superoxide dismutase, catalase,	1617:1663	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	12	26	theme	dismutase	1644:1652	arg1	level					1577:1581	the level	1573:1581	the level of total antioxidant capacity	1573:1611	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	0	27	from	activities	36:45	arg1	parts					81:85	different parts	71:85	different parts of Codonopsis pilosula var	71:112	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	9	28	from	Ara	974:976	arg1	ratio					1020:1024	a ratio	1018:1024	a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7	1018:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	28	from	Ara	974:976	arg1	ratio					1104:1108	a ratio	1102:1108	a ratio of 5.8: 8.9: 8.0: 77.0	1102:1131	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	0	29	theme	Structural	0:9	arg1	features					11:18	Structural features	0:18	Structural features	0:18	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	13	30	theme	antioxidant	2012:2022	arg1	ability					2024:2030	the antioxidant ability	2008:2030	the antioxidant ability of Caenorhabditis elegans	2008:2056	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	13	31	dep	powerful	1750:1757	arg1	antioxidant					1759:1769	antioxidant	1759:1769	antioxidant	1759:1769	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	3	32	theme	acidic	165:170	arg1	polysaccharides					172:186	three acidic polysaccharides	159:186	three acidic polysaccharides from different plant parts of Codonopsis pilosula var	159:240	In this study, three acidic polysaccharides from different plant parts of Codonopsis pilosula var.
36091763	6	33	theme	antioxidant	468:478	arg1	poor					560:563	poor	560:563	poor	560:563	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	33	theme	antioxidant	468:478	arg1	activities					480:489	the antioxidant activities	464:489	the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1)	464:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	0	34	theme	polysaccharides	50:64	arg1	features					11:18	Structural features	0:18	Structural features	0:18	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	0	34	theme	polysaccharides	50:64	arg1	activities					36:45	antioxidant activities	24:45	antioxidant activities	24:45	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	3	35	from	parts	209:213	arg1	polysaccharides					172:186	three acidic polysaccharides	159:186	three acidic polysaccharides from different plant parts of Codonopsis pilosula var	159:240	In this study, three acidic polysaccharides from different plant parts of Codonopsis pilosula var.
36091763	12	36	theme	total	1586:1590	arg1	capacity					1604:1611	total antioxidant capacity	1586:1611	total antioxidant capacity	1586:1611	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	8	37	theme	CLRP-1and	870:878	arg1	CLSP-1					880:885	CLRP-1and CLSP-1	870:885	CLRP-1and CLSP-1	870:885	As revealed by the results, the molecular weight of CLRP-1and CLSP-1 were 15.9 kDa and 26.4 kDa, respectively.
36091763	6	38	theme	experimental	442:453	arg1	results					455:461	the preliminary experimental results	426:461	the preliminary experimental results	426:461	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	10	39	link	4-linked	1275:1282	arg1	1					1272:1272	1	1272:1272	1	1272:1272	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	10	39	link	4-linked	1275:1282	arg1	acid					1297:1300	4-linked galacturonic acid	1275:1300	4-linked galacturonic acid with long homogalacturonan regions	1275:1335	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	13	40	theme	oxygen	1952:1957	arg1	species					1959:1965	reactive oxygen species	1943:1965	reactive oxygen species	1943:1965	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	12	41	theme	capacity	1604:1611	arg1	activity					1621:1628	the activity	1617:1628	the activity of superoxide dismutase, catalase,	1617:1663	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	12	41	theme	capacity	1604:1611	arg1	level					1577:1581	the level	1573:1581	the level of total antioxidant capacity	1573:1611	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	10	42	theme	galacturonic	1284:1295	arg1	1					1272:1272	1	1272:1272	1	1272:1272	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	10	42	theme	galacturonic	1284:1295	arg1	acid					1297:1300	4-linked galacturonic acid	1275:1300	4-linked galacturonic acid with long homogalacturonan regions	1275:1335	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	9	43	from	Xyl	989:991	arg1	ratio					1020:1024	a ratio	1018:1024	a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7	1018:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	43	from	Xyl	989:991	arg1	ratio					1104:1108	a ratio	1102:1108	a ratio of 5.8: 8.9: 8.0: 77.0	1102:1131	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	44	theme	3.8	1029:1031	arg1	ratio					1020:1024	a ratio	1018:1024	a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7	1018:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	6	45	theme	fibrous	532:538	arg1	CLFP-1					547:552	CLFP-1	547:552	CLFP-1	547:552	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	45	theme	fibrous	532:538	arg1	roots					540:544	fibrous roots	532:544	fibrous roots (CLFP-1)	532:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	5	46	theme	polysaccharides	381:395	arg1	yields					359:364	the yields	355:364	the yields of these three polysaccharides	355:395	L. T. Shen were obtained by ion exchange chromatography and gel filtration chromatography, and the yields of these three polysaccharides were different.
36091763	5	46	theme	polysaccharides	381:395	arg1	different					402:410	different	402:410	different	402:410	L. T. Shen were obtained by ion exchange chromatography and gel filtration chromatography, and the yields of these three polysaccharides were different.
36091763	7	47	theme	aerial	679:684	arg1	CLSP-1					693:698	CLSP-1	693:698	CLSP-1	693:698	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	47	theme	aerial	679:684	arg1	parts					686:690	aerial parts	679:690	aerial parts (CLSP-1)	679:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	9	48	dep	Ara	974:976	arg1	in					1133:1134	in	1133:1134	in	1133:1134	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	10	49	theme	homogalacturonan	1312:1327	arg1	regions					1329:1335	long homogalacturonan regions	1307:1335	long homogalacturonan regions	1307:1335	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	3	50	theme	plant	203:207	arg1	parts					209:213	different plant parts	193:213	different plant parts of Codonopsis pilosula var	193:240	In this study, three acidic polysaccharides from different plant parts of Codonopsis pilosula var.
36091763	12	51	theme	antioxidant	1522:1532	arg1	activity					1534:1541	antioxidant activity	1522:1541	antioxidant activity	1522:1541	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	6	52	theme	polysaccharides	498:512	arg1	poor					560:563	poor	560:563	poor	560:563	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	52	theme	polysaccharides	498:512	arg1	activities					480:489	the antioxidant activities	464:489	the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1)	464:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	53	from	rhizomes	519:526	arg1	poor					560:563	poor	560:563	poor	560:563	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	53	from	rhizomes	519:526	arg1	activities					480:489	the antioxidant activities	464:489	the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1)	464:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	6	53	from	rhizomes	519:526	arg1	polysaccharides					498:512	the polysaccharides	494:512	the polysaccharides from rhizomes and fibrous roots (CLFP-1)	494:553	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	5	54	theme	exchange	292:299	arg1	chromatography					301:314	ion exchange chromatography	288:314	ion exchange chromatography	288:314	L. T. Shen were obtained by ion exchange chromatography and gel filtration chromatography, and the yields of these three polysaccharides were different.
36091763	13	55	theme	enzymes	1903:1909	arg1	activities					1911:1920	antioxidant enzymes activities	1891:1920	antioxidant enzymes activities	1891:1920	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	7	56	from	parts	686:690	arg1	polysaccharides					639:653	polysaccharides	639:653	polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1)	639:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	56	from	parts	686:690	arg1	features					627:634	the structural features	612:634	the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1)	612:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	56	from	parts	686:690	arg1	object					710:715	the object	706:715	the object for this study	706:730	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	0	57	from	parts	81:85	arg1	polysaccharides					50:64	polysaccharides	50:64	polysaccharides from different parts of Codonopsis pilosula var	50:112	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	0	57	from	parts	81:85	arg1	features					11:18	Structural features	0:18	Structural features	0:18	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	0	57	from	parts	81:85	arg1	activities					36:45	antioxidant activities	24:45	antioxidant activities	24:45	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	12	58	dep	promoted	1494:1501	arg1	decrease					1669:1676	decrease	1669:1676	decrease the content of malondialdehyde	1669:1707	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	12	58	dep	promoted	1494:1501	arg1	increase					1564:1571	increase	1564:1571	increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase,	1564:1663	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	13	59	theme	factor	1875:1880	arg1	localization					1838:1849	the nuclear localization	1826:1849	the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities	1826:1920	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	4	60	dep	Modesta	243:249	arg1	Nannf					252:256	Nannf	252:256	Nannf.	252:257	Modesta (Nannf.)
36091763	13	61	theme	elegans	2050:2056	arg1	ability					2024:2030	the antioxidant ability	2008:2030	the antioxidant ability of Caenorhabditis elegans	2008:2056	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	7	62	from	features	627:634	arg1	CLRP-1					667:672	CLRP-1	667:672	CLRP-1	667:672	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	62	from	features	627:634	arg1	roots					660:664	roots	660:664	roots (CLRP-1)	660:673	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	62	from	features	627:634	arg1	parts					686:690	aerial parts	679:690	aerial parts (CLSP-1)	679:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	62	from	features	627:634	arg1	CLSP-1					693:698	CLSP-1	693:698	CLSP-1	693:698	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	1	63	dep	modesta	115:121	arg1	Nannf					124:128	Nannf	124:128	Nannf.	124:129	modesta (Nannf.)
36091763	12	64	theme	antioxidant	1424:1434	arg1	assay					1436:1440	The antioxidant assay	1420:1440	The antioxidant assay in IPEC-J2 cells	1420:1457	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	5	65	theme	filtration	324:333	arg1	chromatography					335:348	gel filtration chromatography	320:348	gel filtration chromatography	320:348	L. T. Shen were obtained by ion exchange chromatography and gel filtration chromatography, and the yields of these three polysaccharides were different.
36091763	0	66	from	features	11:18	arg1	parts					81:85	different parts	71:85	different parts of Codonopsis pilosula var	71:112	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	10	67	theme	elucidation	1174:1184	arg1	results					1152:1158	The results	1148:1158	The results of structural elucidation	1148:1184	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	3	68	dep	polysaccharides	172:186	arg1	study					152:156	this study	147:156	this study	147:156	In this study, three acidic polysaccharides from different plant parts of Codonopsis pilosula var.
36091763	0	69	theme	var	110:112	arg1	parts					81:85	different parts	71:85	different parts of Codonopsis pilosula var	71:112	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	13	70	theme	DAF-16	1854:1859	arg1	factor					1875:1880	DAF-16 transcription factor	1854:1880	DAF-16 transcription factor	1854:1880	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	7	71	from	roots	660:664	arg1	polysaccharides					639:653	polysaccharides	639:653	polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1)	639:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	71	from	roots	660:664	arg1	features					627:634	the structural features	612:634	the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1)	612:699	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	7	71	from	roots	660:664	arg1	object					710:715	the object	706:715	the object for this study	706:730	Due to this the structural features of polysaccharides from roots (CLRP-1) and aerial parts (CLSP-1) were the object for this study and were structurally characterized, and their antioxidant activities were evaluated.
36091763	12	72	theme	malondialdehyde	1693:1707	arg1	content					1682:1688	the content	1678:1688	the content of malondialdehyde	1678:1707	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	5	73	theme	gel	320:322	arg1	chromatography					335:348	gel filtration chromatography	320:348	gel filtration chromatography	320:348	L. T. Shen were obtained by ion exchange chromatography and gel filtration chromatography, and the yields of these three polysaccharides were different.
36091763	9	74	theme	CLRP-1	963:968	arg1	Ara					974:976	Ara	974:976	Ara	974:976	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	9	74	theme	CLRP-1	963:968	arg1	composition					948:958	The monosaccharide composition	929:958	The monosaccharide composition of CLRP-1	929:968	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	0	75	theme	antioxidant	24:34	arg1	activities					36:45	antioxidant activities	24:45	antioxidant activities	24:45	Structural features and antioxidant activities of polysaccharides from different parts of Codonopsis pilosula var.
36091763	10	76	theme	pectic	1229:1234	arg1	polysaccharides					1236:1250	pectic polysaccharides	1229:1250	pectic polysaccharides	1229:1250	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	13	77	theme	Caenorhabditis	2035:2048	arg1	elegans					2050:2056	Caenorhabditis elegans	2035:2056	Caenorhabditis elegans	2035:2056	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	12	78	theme	superoxide	1633:1642	arg1	catalase					1655:1662	catalase	1655:1662	catalase	1655:1662	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	12	78	theme	superoxide	1633:1642	arg1	dismutase					1644:1652	superoxide dismutase	1633:1652	superoxide dismutase	1633:1652	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	9	79	dep	3.8	1029:1031	arg1	66.7					1069:1072	66.7	1069:1072	66.7	1069:1072	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	11	80	theme	Arabinogalactan	1338:1352	arg1	chains					1412:1417	side chains	1407:1417	side chains	1407:1417	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	11	80	theme	Arabinogalactan	1338:1352	arg1	type					1354:1357	Arabinogalactan type I	1338:1359	Arabinogalactan type I	1338:1359	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	11	80	theme	Arabinogalactan	1338:1352	arg1	type					1381:1384	arabinogalactan type II	1365:1387	arabinogalactan type II	1365:1387	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	12	81	theme	antioxidant	1592:1602	arg1	capacity					1604:1611	total antioxidant capacity	1586:1611	total antioxidant capacity	1586:1611	The antioxidant assay in IPEC-J2 cells showed that both CLRP-1 and CLSP-1 promoted cell viability and antioxidant activity, which significantly increase the level of total antioxidant capacity and the activity of superoxide dismutase, catalase, and decrease the content of malondialdehyde.
36091763	8	82	theme	molecular	850:858	arg1	15.9 kDa					892:899	15.9 kDa	892:899	15.9 kDa	892:899	As revealed by the results, the molecular weight of CLRP-1and CLSP-1 were 15.9 kDa and 26.4 kDa, respectively.
36091763	8	82	theme	molecular	850:858	arg1	weight					860:865	the molecular weight	846:865	the molecular weight of CLRP-1and CLSP-1	846:885	As revealed by the results, the molecular weight of CLRP-1and CLSP-1 were 15.9 kDa and 26.4 kDa, respectively.
36091763	14	83	theme	potential	2127:2135	arg1	CLSP-1					2103:2108	CLSP-1	2103:2108	CLSP-1	2103:2108	Thus, these finding suggest that CLRP-1 and CLSP-1 could be used as potential antioxidants.
36091763	14	83	theme	potential	2127:2135	arg1	antioxidants					2137:2148	potential antioxidants	2127:2148	potential antioxidants	2127:2148	Thus, these finding suggest that CLRP-1 and CLSP-1 could be used as potential antioxidants.
36091763	14	83	theme	potential	2127:2135	arg1	CLRP-1					2092:2097	CLRP-1	2092:2097	CLRP-1	2092:2097	Thus, these finding suggest that CLRP-1 and CLSP-1 could be used as potential antioxidants.
36091763	13	84	theme	Caenorhabditis	1784:1797	arg1	elegans					1799:1805	Caenorhabditis elegans	1784:1805	Caenorhabditis elegans	1784:1805	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	6	85	theme	preliminary	430:440	arg1	results					455:461	the preliminary experimental results	426:461	the preliminary experimental results	426:461	According to the preliminary experimental results, the antioxidant activities of the polysaccharides from rhizomes and fibrous roots (CLFP-1) were poor, and was thus not studied further.
36091763	11	86	theme	arabinogalactan	1365:1379	arg1	chains					1412:1417	side chains	1407:1417	side chains	1407:1417	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	11	86	theme	arabinogalactan	1365:1379	arg1	type					1354:1357	Arabinogalactan type I	1338:1359	Arabinogalactan type I	1338:1359	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	11	86	theme	arabinogalactan	1365:1379	arg1	type					1381:1384	arabinogalactan type II	1365:1387	arabinogalactan type II	1365:1387	Arabinogalactan type I and arabinogalactan type II were presented as side chains.
36091763	9	87	theme	77.0	1128:1131	arg1	ratio					1104:1108	a ratio	1102:1108	a ratio of 5.8: 8.9: 8.0: 77.0	1102:1131	The monosaccharide composition of CLRP-1 was Ara, Rha, Fuc, Xyl, Man, Gal, GlcA, GalA in a ratio of 3.8: 8.4: 1.0: 0.8: 2.4: 7.4: 7.5: 2.0: 66.7, and Ara, Rha, Gal, GalA in a ratio of 5.8: 8.9: 8.0: 77.0 in for CLSP-1.
36091763	8	88	theme	CLSP-1	880:885	arg1	15.9 kDa					892:899	15.9 kDa	892:899	15.9 kDa	892:899	As revealed by the results, the molecular weight of CLRP-1and CLSP-1 were 15.9 kDa and 26.4 kDa, respectively.
36091763	8	88	theme	CLSP-1	880:885	arg1	weight					860:865	the molecular weight	846:865	the molecular weight of CLRP-1and CLSP-1	846:885	As revealed by the results, the molecular weight of CLRP-1and CLSP-1 were 15.9 kDa and 26.4 kDa, respectively.
36091763	10	89	theme	4-linked	1275:1282	arg1	1					1272:1272	1	1272:1272	1	1272:1272	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	10	89	theme	4-linked	1275:1282	arg1	acid					1297:1300	4-linked galacturonic acid	1275:1300	4-linked galacturonic acid with long homogalacturonan regions	1275:1335	The results of structural elucidation indicated that both CLRP-1 and CLSP-1 were pectic polysaccharides, mainly composed of 1, 4-linked galacturonic acid with long homogalacturonan regions.
36091763	13	90	theme	powerful	1750:1757	arg1	abilities					1771:1779	powerful antioxidant abilities	1750:1779	powerful antioxidant abilities	1750:1779	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36091763	13	91	theme	malondialdehyde	1971:1985	arg1	contents					1987:1994	malondialdehyde contents	1971:1994	malondialdehyde contents	1971:1994	Moreover, CLRP-1 and CLSP-1 also showed powerful antioxidant abilities in Caenorhabditis elegans and might regulate the nuclear localization of DAF-16 transcription factor, induced antioxidant enzymes activities, and further reduced reactive oxygen species and malondialdehyde contents to increase the antioxidant ability of Caenorhabditis elegans.
36986542	3	0	from	action	425:430	arg1	toxicity					543:550	H2O2-induced toxicity	530:550	H2O2-induced toxicity in IEC-6 cells	530:565	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	0	from	action	425:430	arg1	alga					495:498	Tunisian red alga	482:498	Tunisian red alga	482:498	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	0	from	action	425:430	arg1	spinosum					510:517	Gelidium spinosum	501:517	Gelidium spinosum (PSGS)	501:524	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	7	1	theme	cell	1058:1061	arg1	morphology					1063:1072	normal cell morphology	1051:1072	normal cell morphology	1051:1072	Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H2O2-callenged cells.
36986542	9	2	theme	structural	1277:1286	arg1	composition					1288:1298	its structural composition	1273:1298	its structural composition	1273:1298	This protection mechanism of PSGS may be associated with its structural composition.
36986542	11	3	from	investment	1611:1620	arg1	diseases					1666:1673	handling intestinal diseases	1646:1673	handling intestinal diseases	1646:1673	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	6	4	theme	normal	952:957	arg1	cells					959:963	normal cells	952:963	normal cells	952:963	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	9	5	theme	PSGS	1245:1248	arg1	mechanism					1232:1240	This protection mechanism	1216:1240	This protection mechanism of PSGS	1216:1248	This protection mechanism of PSGS may be associated with its structural composition.
36986542	1	6	theme	unpredictable	155:167	arg1	injury					132:137	Gut injury	128:137	Gut injury	128:137	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	1	6	theme	unpredictable	155:167	arg1	illness					169:175	a severe and unpredictable illness	142:175	a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs)	142:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	0	7	theme	Epithelial	101:110	arg1	Apoptosis					117:125	Intestinal Epithelial Cell Apoptosis	90:125	Intestinal Epithelial Cell Apoptosis	90:125	The Potential Effect of Polysaccharides Extracted from Red Alga Gelidium spinosum against Intestinal Epithelial Cell Apoptosis.
36986542	6	8	theme	antioxidant	873:883	arg1	defense					885:891	the antioxidant defense	869:891	the antioxidant defense	869:891	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	3	9	theme	IEC-6	555:559	arg1	cells					561:565	IEC-6 cells	555:565	IEC-6 cells	555:565	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	4	10	theme	PSGS	670:673	arg1	concentrations					643:656	convenient concentrations	632:656	convenient concentrations of H2O2 and PSGS	632:673	The cell viability test was initially carried out to screen out convenient concentrations of H2O2 and PSGS.
36986542	0	11	theme	Intestinal	90:99	arg1	Apoptosis					117:125	Intestinal Epithelial Cell Apoptosis	90:125	Intestinal Epithelial Cell Apoptosis	90:125	The Potential Effect of Polysaccharides Extracted from Red Alga Gelidium spinosum against Intestinal Epithelial Cell Apoptosis.
36986542	7	12	theme	normal	1051:1056	arg1	morphology					1063:1072	normal cell morphology	1051:1072	normal cell morphology	1051:1072	Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H2O2-callenged cells.
36986542	11	13	theme	protective	1573:1582	arg1	functions					1584:1592	the protective functions	1569:1592	the protective functions	1569:1592	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	2	14	theme	cell	276:279	arg1	death					281:285	Excessive IEC apoptotic cell death	252:285	Excessive IEC apoptotic cell death during the pathophysiological state	252:321	Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases.
36986542	8	15	theme	dismutase	1138:1146	arg1	activities					1161:1170	superoxide dismutase and catalase activities	1127:1170	superoxide dismutase and catalase activities	1127:1170	PSGS also equally sustained superoxide dismutase and catalase activities and hindered the apoptosis induced by H2O2.
36986542	11	16	theme	handling	1646:1653	arg1	diseases					1666:1673	handling intestinal diseases	1646:1673	handling intestinal diseases	1646:1673	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	4	17	theme	convenient	632:641	arg1	concentrations					643:656	convenient concentrations	632:656	convenient concentrations of H2O2 and PSGS	632:673	The cell viability test was initially carried out to screen out convenient concentrations of H2O2 and PSGS.
36986542	2	18	theme	apoptotic	266:274	arg1	death					281:285	Excessive IEC apoptotic cell death	252:285	Excessive IEC apoptotic cell death during the pathophysiological state	252:321	Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases.
36986542	3	19	theme	polysaccharides	461:475	arg1	action					425:430	cytoprotective action	410:430	cytoprotective action	410:430	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	19	theme	polysaccharides	461:475	arg1	mechanisms					447:456	underlying mechanisms	436:456	underlying mechanisms	436:456	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	0	20	theme	Cell	112:115	arg1	Apoptosis					117:125	Intestinal Epithelial Cell Apoptosis	90:125	Intestinal Epithelial Cell Apoptosis	90:125	The Potential Effect of Polysaccharides Extracted from Red Alga Gelidium spinosum against Intestinal Epithelial Cell Apoptosis.
36986542	11	21	theme	natural	1625:1631	arg1	resources					1633:1641	natural resources	1625:1641	natural resources	1625:1641	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	3	22	dep	action	425:430	arg1	the					406:408	the	406:408	the	406:408	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	2	23	theme	IEC	262:264	arg1	death					281:285	Excessive IEC apoptotic cell death	252:285	Excessive IEC apoptotic cell death during the pathophysiological state	252:321	Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases.
36986542	10	24	theme	visible	1317:1323	arg1	spectrum					1325:1332	The ultraviolet visible spectrum	1301:1332	The ultraviolet visible spectrum	1301:1332	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	1	25	theme	related	177:183	arg1	injury					132:137	Gut injury	128:137	Gut injury	128:137	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	1	25	theme	related	177:183	arg1	illness					169:175	a severe and unpredictable illness	142:175	a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs)	142:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	0	26	theme	Potential	4:12	arg1	Effect					14:19	The Potential Effect	0:19	The Potential Effect of Polysaccharides	0:38	The Potential Effect of Polysaccharides Extracted from Red Alga Gelidium spinosum against Intestinal Epithelial Cell Apoptosis.
36986542	4	27	theme	cell	572:575	arg1	test					587:590	The cell viability test	568:590	The cell viability test	568:590	The cell viability test was initially carried out to screen out convenient concentrations of H2O2 and PSGS.
36986542	10	28	theme	liquid	1419:1424	arg1	HPLC					1442:1445	HPLC	1442:1445	HPLC	1442:1445	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	10	28	theme	liquid	1419:1424	arg1	chromatography					1426:1439	high-performance liquid chromatography	1402:1439	high-performance liquid chromatography (HPLC)	1402:1446	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	7	29	theme	cell	997:1000	arg1	viability					1002:1010	cell viability	997:1010	cell viability	997:1010	Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H2O2-callenged cells.
36986542	6	30	theme	IEC-6	930:934	arg1	cells					936:940	IEC-6 cells	930:940	IEC-6 cells	930:940	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	8	31	theme	superoxide	1127:1136	arg1	dismutase					1138:1146	superoxide dismutase	1127:1146	superoxide dismutase	1127:1146	PSGS also equally sustained superoxide dismutase and catalase activities and hindered the apoptosis induced by H2O2.
36986542	10	32	theme	high-performance	1402:1417	arg1	HPLC					1442:1445	HPLC	1442:1445	HPLC	1442:1445	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	10	32	theme	high-performance	1402:1417	arg1	chromatography					1426:1439	high-performance liquid chromatography	1402:1439	high-performance liquid chromatography (HPLC)	1402:1446	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	10	33	theme	X-ray	1373:1377	arg1	XRD					1392:1394	XRD	1392:1394	XRD	1392:1394	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	10	33	theme	X-ray	1373:1377	arg1	diffraction					1379:1389	X-ray diffraction	1373:1389	X-ray diffraction (XRD)	1373:1395	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	2	34	theme	pathophysiological	298:315	arg1	state					317:321	the pathophysiological state	294:321	the pathophysiological state	294:321	Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases.
36986542	8	35	theme	catalase	1152:1159	arg1	activities					1161:1170	superoxide dismutase and catalase activities	1127:1170	superoxide dismutase and catalase activities	1127:1170	PSGS also equally sustained superoxide dismutase and catalase activities and hindered the apoptosis induced by H2O2.
36986542	11	36	theme	resources	1633:1641	arg1	investment					1611:1620	the investment	1607:1620	the investment of natural resources in handling intestinal diseases	1607:1673	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	7	37	theme	H2O2-callenged	1077:1090	arg1	cells					1092:1096	H2O2-callenged cells	1077:1096	H2O2-callenged cells	1077:1096	Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H2O2-callenged cells.
36986542	1	38	theme	increased	192:200	arg1	death					207:211	the increased cell death	188:211	the increased cell death of intestinal epithelial cells (IECs)	188:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	4	39	theme	viability	577:585	arg1	test					587:590	The cell viability test	568:590	The cell viability test	568:590	The cell viability test was initially carried out to screen out convenient concentrations of H2O2 and PSGS.
36986542	5	40	theme	40	712:713	arg1	µM					715:716	µM	715:716	µM	715:716	Subsequently, cells were exposed to 40 µM H2O2 over 4 h in the presence or absence of PSGS.
36986542	6	41	theme	cell	843:846	arg1	mortality					848:856	over 70% cell mortality	834:856	over 70% cell mortality	834:856	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	6	42	dep	caused	796:801	arg1	disturbed					859:867	disturbed	859:867	disturbed the antioxidant defense	859:891	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	6	42	dep	caused	796:801	arg1	increased					898:906	increased	898:906	increased the apoptotic rate in IEC-6 cells (32% than normal cells)	898:964	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	1	43	theme	cell	202:205	arg1	death					207:211	the increased cell death	188:211	the increased cell death of intestinal epithelial cells (IECs)	188:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	2	44	theme	inflammatory	339:350	arg1	diseases					352:359	chronic inflammatory diseases	331:359	chronic inflammatory diseases	331:359	Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases.
36986542	5	45	theme	µM	715:716	arg1	H2O2					718:721	40 µM H2O2	712:721	40 µM H2O2	712:721	Subsequently, cells were exposed to 40 µM H2O2 over 4 h in the presence or absence of PSGS.
36986542	4	46	theme	H2O2	661:664	arg1	concentrations					643:656	convenient concentrations	632:656	convenient concentrations of H2O2 and PSGS	632:673	The cell viability test was initially carried out to screen out convenient concentrations of H2O2 and PSGS.
36986542	7	47	from	µg/mL	1041:1045	arg1	cells					1092:1096	H2O2-callenged cells	1077:1096	H2O2-callenged cells	1077:1096	Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H2O2-callenged cells.
36986542	6	48	dep	mortality	848:856	arg1	%					841:841	%	841:841	%	841:841	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	3	49	theme	Tunisian	482:489	arg1	alga					495:498	Tunisian red alga	482:498	Tunisian red alga	482:498	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	49	theme	Tunisian	482:489	arg1	spinosum					510:517	Gelidium spinosum	501:517	Gelidium spinosum (PSGS)	501:524	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	0	50	theme	Polysaccharides	24:38	arg1	Effect					14:19	The Potential Effect	0:19	The Potential Effect of Polysaccharides	0:38	The Potential Effect of Polysaccharides Extracted from Red Alga Gelidium spinosum against Intestinal Epithelial Cell Apoptosis.
36986542	3	51	theme	underlying	436:445	arg1	mechanisms					447:456	underlying mechanisms	436:456	underlying mechanisms	436:456	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	6	52	dep	rate	922:925	arg1	%					945:945	32%	943:945	32%	943:945	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	10	53	theme	ultraviolet	1305:1315	arg1	spectrum					1325:1332	The ultraviolet visible spectrum	1301:1332	The ultraviolet visible spectrum	1301:1332	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	3	54	theme	red	491:493	arg1	alga					495:498	Tunisian red alga	482:498	Tunisian red alga	482:498	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	54	theme	red	491:493	arg1	spinosum					510:517	Gelidium spinosum	501:517	Gelidium spinosum (PSGS)	501:524	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	0	55	theme	Red	55:57	arg1	Alga					59:62	Red Alga	55:62	Red Alga	55:62	The Potential Effect of Polysaccharides Extracted from Red Alga Gelidium spinosum against Intestinal Epithelial Cell Apoptosis.
36986542	6	56	theme	apoptotic	912:920	arg1	rate					922:925	the apoptotic rate	908:925	the apoptotic rate in IEC-6 cells (32% than normal cells)	908:964	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	10	57	theme	sulfated	1481:1488	arg1	polysaccharides					1490:1504	sulfated polysaccharides	1481:1504	sulfated polysaccharides	1481:1504	The ultraviolet visible spectrum, Fourier-transformed infrared (FT-IR), X-ray diffraction (XRD), and high-performance liquid chromatography (HPLC) demonstrated that PSGS is mainly sulfated polysaccharides.
36986542	7	58	from	morphology	1063:1072	arg1	cells					1092:1096	H2O2-callenged cells	1077:1096	H2O2-callenged cells	1077:1096	Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H2O2-callenged cells.
36986542	5	59	dep	presence	739:746	arg1	the					735:737	the	735:737	the	735:737	Subsequently, cells were exposed to 40 µM H2O2 over 4 h in the presence or absence of PSGS.
36986542	3	60	theme	Gelidium	501:508	arg1	PSGS					520:523	PSGS	520:523	PSGS	520:523	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	60	theme	Gelidium	501:508	arg1	alga					495:498	Tunisian red alga	482:498	Tunisian red alga	482:498	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	60	theme	Gelidium	501:508	arg1	spinosum					510:517	Gelidium spinosum	501:517	Gelidium spinosum (PSGS)	501:524	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	61	from	alga	495:498	arg1	polysaccharides					461:475	polysaccharides	461:475	polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS),	461:525	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	61	from	alga	495:498	arg1	action					425:430	cytoprotective action	410:430	cytoprotective action	410:430	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	61	from	alga	495:498	arg1	mechanisms					447:456	underlying mechanisms	436:456	underlying mechanisms	436:456	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	1	62	theme	Gut	128:130	arg1	injury					132:137	Gut injury	128:137	Gut injury	128:137	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	1	62	theme	Gut	128:130	arg1	illness					169:175	a severe and unpredictable illness	142:175	a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs)	142:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	6	63	dep	%	841:841	arg1	70					839:840	70	839:840	70	839:840	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	6	64	from	rate	922:925	arg1	cells					936:940	IEC-6 cells	930:940	IEC-6 cells	930:940	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	2	65	theme	Excessive	252:260	arg1	death					281:285	Excessive IEC apoptotic cell death	252:285	Excessive IEC apoptotic cell death during the pathophysiological state	252:321	Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases.
36986542	11	66	theme	deeper	1549:1554	arg1	insight					1556:1562	a deeper insight	1547:1562	a deeper insight into the protective functions	1547:1592	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	2	67	theme	chronic	331:337	arg1	diseases					352:359	chronic inflammatory diseases	331:359	chronic inflammatory diseases	331:359	Excessive IEC apoptotic cell death during the pathophysiological state entails chronic inflammatory diseases.
36986542	11	68	theme	research	1524:1531	arg1	work					1533:1536	this research work	1519:1536	this research work	1519:1536	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	1	69	theme	intestinal	216:225	arg1	cells					238:242	intestinal epithelial cells	216:242	intestinal epithelial cells (IECs)	216:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	1	69	theme	intestinal	216:225	arg1	IECs					245:248	IECs	245:248	IECs	245:248	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	7	70	theme	PSGS	983:986	arg1	Pretreatment					967:978	Pretreatment	967:978	Pretreatment of PSGS	967:986	Pretreatment of PSGS restored cell viability, especially when used at 150 µg/mL and normal cell morphology in H2O2-callenged cells.
36986542	9	71	theme	protection	1221:1230	arg1	mechanism					1232:1240	This protection mechanism	1216:1240	This protection mechanism of PSGS	1216:1248	This protection mechanism of PSGS may be associated with its structural composition.
36986542	3	72	from	mechanisms	447:456	arg1	toxicity					543:550	H2O2-induced toxicity	530:550	H2O2-induced toxicity in IEC-6 cells	530:565	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	72	from	mechanisms	447:456	arg1	alga					495:498	Tunisian red alga	482:498	Tunisian red alga	482:498	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	72	from	mechanisms	447:456	arg1	spinosum					510:517	Gelidium spinosum	501:517	Gelidium spinosum (PSGS)	501:524	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	3	73	theme	H2O2-induced	530:541	arg1	toxicity					543:550	H2O2-induced toxicity	530:550	H2O2-induced toxicity in IEC-6 cells	530:565	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	1	74	theme	epithelial	227:236	arg1	cells					238:242	intestinal epithelial cells	216:242	intestinal epithelial cells (IECs)	216:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	1	74	theme	epithelial	227:236	arg1	IECs					245:248	IECs	245:248	IECs	245:248	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	6	75	theme	oxidative	803:811	arg1	stress					813:818	oxidative stress	803:818	oxidative stress manifested by over 70% cell mortality	803:856	Findings revealed that H2O2 caused oxidative stress manifested by over 70% cell mortality, disturbed the antioxidant defense, and increased the apoptotic rate in IEC-6 cells (32% than normal cells).
36986542	11	76	theme	intestinal	1655:1664	arg1	diseases					1666:1673	handling intestinal diseases	1646:1673	handling intestinal diseases	1646:1673	Eventually, this research work provides a deeper insight into the protective functions and enhances the investment of natural resources in handling intestinal diseases.
36986542	1	77	theme	cells	238:242	arg1	death					207:211	the increased cell death	188:211	the increased cell death of intestinal epithelial cells (IECs)	188:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	3	78	theme	cytoprotective	410:423	arg1	action					425:430	cytoprotective action	410:430	cytoprotective action	410:430	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
36986542	5	79	theme	PSGS	762:765	arg1	presence					739:746	presence	739:746	presence	739:746	Subsequently, cells were exposed to 40 µM H2O2 over 4 h in the presence or absence of PSGS.
36986542	5	79	theme	PSGS	762:765	arg1	absence					751:757	absence	751:757	absence	751:757	Subsequently, cells were exposed to 40 µM H2O2 over 4 h in the presence or absence of PSGS.
36986542	1	80	theme	severe	144:149	arg1	injury					132:137	Gut injury	128:137	Gut injury	128:137	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	1	80	theme	severe	144:149	arg1	illness					169:175	a severe and unpredictable illness	142:175	a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs)	142:249	Gut injury is a severe and unpredictable illness related to the increased cell death of intestinal epithelial cells (IECs).
36986542	3	81	from	toxicity	543:550	arg1	cells					561:565	IEC-6 cells	555:565	IEC-6 cells	555:565	This investigation was undertaken to assess the cytoprotective action and underlying mechanisms of polysaccharides from Tunisian red alga, Gelidium spinosum (PSGS), on H2O2-induced toxicity in IEC-6 cells.
35520274	6	0	theme	arabinose	1274:1282	arg1	ratios					1264:1269	the molar ratios	1254:1269	the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI	1254:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	1	1	theme	increasing	254:263	arg1	attention					265:273	increasing attention	254:273	increasing attention	254:273	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	3	2	from	stages	657:662	arg1	stabilities					612:622	its digestive stabilities	598:622	its digestive stabilities at different simulated digestion stages	598:662	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	3	3	theme	upper	547:551	arg1	tract					570:574	the human upper gastrointestinal tract	537:574	the human upper gastrointestinal tract	537:574	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	7	4	theme	beneficial	1717:1726	arg1	Megamonas					1778:1786	Megamonas	1778:1786	Megamonas	1778:1786	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	4	theme	beneficial	1717:1726	arg1	Collinsella					1793:1803	Collinsella	1793:1803	Collinsella	1793:1803	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	4	theme	beneficial	1717:1726	arg1	Bifidobacterium					1761:1775	Bifidobacterium	1761:1775	Bifidobacterium	1761:1775	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	4	theme	beneficial	1717:1726	arg1	microbes					1728:1735	several beneficial microbes	1709:1735	several beneficial microbes	1709:1735	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	4	theme	beneficial	1717:1726	arg1	Bacteroides					1748:1758	Bacteroides	1748:1758	Bacteroides	1748:1758	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	8	5	theme	potential	2108:2116	arg1	LLP					2087:2089	LLP	2087:2089	LLP	2087:2089	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	8	5	theme	potential	2108:2116	arg1	prebiotic					2118:2126	a potential prebiotic	2106:2126	a potential prebiotic for the improvement of intestinal health	2106:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	0	6	theme	In	0:1	arg1	characteristics					19:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	1	7	theme	abundant	203:210	arg1	components					212:221	the most abundant components	194:221	the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits	194:324	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	0	8	from	lotus	85:89	arg1	polysaccharides					64:78	polysaccharides	64:78	polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota	64:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	8	from	lotus	85:89	arg1	characteristics					19:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	8	from	lotus	85:89	arg1	degradation					49:59	microbial degradation	39:59	microbial degradation	39:59	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	8	from	lotus	85:89	arg1	modulation					125:134	the modulation	121:134	the modulation of intestinal microbiota	121:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	8	9	theme	intestinal	2151:2160	arg1	health					2162:2167	intestinal health	2151:2167	intestinal health	2151:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	4	10	dep	in	944:945	arg1	vitro					947:951	vitro	947:951	vitro	947:951	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	3	11	theme	digestive	602:610	arg1	stabilities					612:622	its digestive stabilities	598:622	its digestive stabilities at different simulated digestion stages	598:662	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	1	12	from	components	212:221	arg1	lotus					226:230	lotus leaves	226:237	lotus leaves	226:237	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	7	13	theme	propionic	1898:1906	arg1	acids					1872:1876	several short-chain fatty acids	1846:1876	several short-chain fatty acids	1846:1876	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	13	theme	propionic	1898:1906	arg1	acids					1921:1925	acetic, propionic, and butyric acids	1890:1925	acetic, propionic, and butyric acids	1890:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	0	14	theme	microbiota	150:159	arg1	modulation					125:134	the modulation	121:134	the modulation of intestinal microbiota	121:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	15	from	effects	110:116	arg1	polysaccharides					64:78	polysaccharides	64:78	polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota	64:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	15	from	effects	110:116	arg1	characteristics					19:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	15	from	effects	110:116	arg1	degradation					49:59	microbial degradation	39:59	microbial degradation	39:59	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	15	from	effects	110:116	arg1	modulation					125:134	the modulation	121:134	the modulation of intestinal microbiota	121:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	6	16	from	LLPI	1494:1497	arg1	faster					1533:1538	faster	1533:1538	faster	1533:1538	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	6	16	from	LLPI	1494:1497	arg1	utilization					1452:1462	the utilization	1448:1462	the utilization of arabinose and galactose in LLPI by intestinal microbiota	1448:1522	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	4	17	theme	degradation	901:911	arg1	characteristics					913:927	the microbial degradation characteristics	887:927	the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum	887:988	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	6	18	theme	initial	1578:1584	arg1	stage					1599:1603	the initial fermentation stage	1574:1603	the initial fermentation stage	1574:1603	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	3	19	from	tract	570:574	arg1	stable					527:532	stable	527:532	stable	527:532	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	5	20	theme	sugars	1053:1058	arg1	content					1033:1039	the content	1029:1039	the content of reducing sugars released from LLPI	1029:1077	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	3	21	theme	simulated	637:645	arg1	stages					657:662	different simulated digestion stages	627:662	different simulated digestion stages	627:662	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	7	22	theme	gut	1651:1653	arg1	composition					1665:1675	gut microbial composition	1651:1675	gut microbial composition	1651:1675	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	4	23	theme	fecal	975:979	arg1	inoculum					981:988	human fecal inoculum	969:988	human fecal inoculum	969:988	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	3	24	from	vitro	579:583	arg1	stable					527:532	stable	527:532	stable	527:532	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	0	25	theme	polysaccharides	64:78	arg1	characteristics					19:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	25	theme	polysaccharides	64:78	arg1	degradation					49:59	microbial degradation	39:59	microbial degradation	39:59	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	8	26	theme	present	1950:1956	arg1	study					1958:1962	the present study	1946:1962	the present study	1946:1962	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	6	27	from	arabinose	1467:1475	arg1	LLPI					1494:1497	LLPI	1494:1497	LLPI	1494:1497	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	5	28	theme	initial	1132:1138	arg1	0-6 h					1160:1164	0-6 h	1160:1164	0-6 h	1160:1164	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	5	28	theme	initial	1132:1138	arg1	stage					1153:1157	the initial fermentation stage	1128:1157	the initial fermentation stage (0-6 h)	1128:1165	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	2	29	dep	in	459:460	arg1	vitro					462:466	vitro	462:466	vitro	462:466	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	4	30	used	utilized	725:732	arg2	LLP					694:696	the indigestible LLP	677:696	the indigestible LLP (LLPI)	677:703	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	4	30	used	utilized	725:732	arg2	LLPI					699:702	LLPI	699:702	LLPI	699:702	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	6	31	from	galactose	1481:1489	arg1	LLPI					1494:1497	LLPI	1494:1497	LLPI	1494:1497	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	6	32	theme	acid	1325:1328	arg1	ratios					1264:1269	the molar ratios	1254:1269	the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI	1254:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	0	33	from	degradation	49:59	arg1	lotus					85:89	lotus leaves	85:96	lotus leaves	85:96	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	33	from	degradation	49:59	arg1	effects					110:116	related effects	102:116	related effects on the modulation of intestinal microbiota	102:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	2	34	theme	polysaccharides	412:426	arg1	characteristics					382:396	the digestive and microbial degradation characteristics	342:396	the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP)	342:432	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	7	35	theme	acids	1872:1876	arg1	generation					1832:1841	the promoted generation	1819:1841	the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids	1819:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	6	36	from	utilization	1452:1462	arg1	LLPI					1494:1497	LLPI	1494:1497	LLPI	1494:1497	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	1	37	theme	health-promoting	300:315	arg1	benefits					317:324	their promising health-promoting benefits	284:324	their promising health-promoting benefits	284:324	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	2	38	theme	lotus	401:405	arg1	LLP					429:431	LLP	429:431	LLP	429:431	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	2	38	theme	lotus	401:405	arg1	polysaccharides					412:426	lotus leaf polysaccharides	401:426	lotus leaf polysaccharides (LLP)	401:432	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	4	39	dep	in	848:849	arg1	vitro					851:855	vitro	851:855	vitro	851:855	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	7	40	theme	short-chain	1854:1864	arg1	acids					1872:1876	several short-chain fatty acids	1846:1876	several short-chain fatty acids	1846:1876	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	40	theme	short-chain	1854:1864	arg1	acids					1921:1925	acetic, propionic, and butyric acids	1890:1925	acetic, propionic, and butyric acids	1890:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	4	41	from	microbiota	748:757	arg1	feces					768:772	human feces	762:772	human feces	762:772	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	0	42	dep	lotus	85:89	arg1	leaves					91:96	leaves	91:96	leaves	91:96	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	8	43	theme	digestive	2007:2015	arg1	characteristics					2043:2057	the digestive and microbial degradation characteristics	2003:2057	the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health	2003:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	4	44	theme	human	762:766	arg1	feces					768:772	human feces	762:772	human feces	762:772	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	0	45	theme	digestive	9:17	arg1	characteristics					19:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics	0:33	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	1	46	theme	promising	290:298	arg1	benefits					317:324	their promising health-promoting benefits	284:324	their promising health-promoting benefits	284:324	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	8	47	theme	microbial	2021:2029	arg1	characteristics					2043:2057	the digestive and microbial degradation characteristics	2003:2057	the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health	2003:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	7	48	theme	promoted	1823:1830	arg1	generation					1832:1841	the promoted generation	1819:1841	the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids	1819:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	6	49	dep	3.72	1387:1390	arg1	to					1384:1385	to	1384:1385	to	1384:1385	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	2	50	theme	digestive	346:354	arg1	characteristics					382:396	the digestive and microbial degradation characteristics	342:396	the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP)	342:432	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	6	51	from	galactose	1291:1299	arg1	LLPI					1340:1343	LLPI	1340:1343	LLPI	1340:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	6	52	dep	1.40	1368:1371	arg1	to					1365:1366	to	1365:1366	to	1365:1366	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	8	53	from	study	1958:1962	arg1	beneficial					1968:1977	beneficial	1968:1977	beneficial	1968:1977	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	8	53	from	study	1958:1962	arg1	findings					1932:1939	The findings	1928:1939	The findings from the present study	1928:1962	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	8	54	theme	LLP	2062:2064	arg1	characteristics					2043:2057	the digestive and microbial degradation characteristics	2003:2057	the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health	2003:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	4	55	dep	in	781:782	arg1	vitro					784:788	vitro	784:788	vitro	784:788	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	0	56	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	6	57	dep	18.71	1411:1415	arg1	to					1408:1409	to	1408:1409	to	1408:1409	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	2	58	theme	microbial	360:368	arg1	characteristics					382:396	the digestive and microbial degradation characteristics	342:396	the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP)	342:432	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	7	59	theme	microbes	1728:1735	arg1	abundances					1695:1704	the abundances	1691:1704	the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella	1691:1803	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	3	60	theme	human	541:545	arg1	tract					570:574	the human upper gastrointestinal tract	537:574	the human upper gastrointestinal tract	537:574	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	4	61	theme	48 h.	873:877	arg1	fermentation					857:868	the in vitro fermentation	844:868	the in vitro fermentation of 48 h.	844:877	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	7	62	theme	several	1709:1715	arg1	Megamonas					1778:1786	Megamonas	1778:1786	Megamonas	1778:1786	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	62	theme	several	1709:1715	arg1	Collinsella					1793:1803	Collinsella	1793:1803	Collinsella	1793:1803	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	62	theme	several	1709:1715	arg1	Bifidobacterium					1761:1775	Bifidobacterium	1761:1775	Bifidobacterium	1761:1775	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	62	theme	several	1709:1715	arg1	microbes					1728:1735	several beneficial microbes	1709:1735	several beneficial microbes	1709:1735	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	62	theme	several	1709:1715	arg1	Bacteroides					1748:1758	Bacteroides	1748:1758	Bacteroides	1748:1758	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	6	63	theme	molar	1258:1262	arg1	ratios					1264:1269	the molar ratios	1254:1269	the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI	1254:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	3	64	from	stable	527:532	arg1	tract					570:574	the human upper gastrointestinal tract	537:574	the human upper gastrointestinal tract	537:574	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	3	64	from	stable	527:532	arg1	vitro					579:583	vitro	579:583	vitro according to its digestive stabilities at different simulated digestion stages	579:662	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	8	65	used	used	2098:2101	arg2	LLP					2087:2089	LLP	2087:2089	LLP	2087:2089	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	8	65	used	used	2098:2101	arg2	prebiotic					2118:2126	a potential prebiotic	2106:2126	a potential prebiotic for the improvement of intestinal health	2106:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	6	66	from	LLPI	1340:1343	arg1	ratios					1264:1269	the molar ratios	1254:1269	the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI	1254:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	4	67	theme	in	848:849	arg1	fermentation					857:868	the in vitro fermentation	844:868	the in vitro fermentation of 48 h.	844:877	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	4	68	theme	indigestible	681:692	arg1	LLPI					699:702	LLPI	699:702	LLPI	699:702	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	4	68	theme	indigestible	681:692	arg1	LLP					694:696	the indigestible LLP	677:696	the indigestible LLP (LLPI)	677:703	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	3	69	theme	gastrointestinal	553:568	arg1	tract					570:574	the human upper gastrointestinal tract	537:574	the human upper gastrointestinal tract	537:574	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	5	70	dep	2.02 × 104 Da	1230:1242	arg1	to					1227:1228	to	1227:1228	to	1227:1228	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	0	71	theme	intestinal	139:148	arg1	microbiota					150:159	intestinal microbiota	139:159	intestinal microbiota	139:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	1	72	theme	components	212:221	arg1	components					212:221	the most abundant components	194:221	the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits	194:324	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	1	72	theme	components	212:221	arg1	one					187:189	one	187:189	one	187:189	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	7	73	theme	butyric	1913:1919	arg1	acids					1872:1876	several short-chain fatty acids	1846:1876	several short-chain fatty acids	1846:1876	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	73	theme	butyric	1913:1919	arg1	acids					1921:1925	acetic, propionic, and butyric acids	1890:1925	acetic, propionic, and butyric acids	1890:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	4	74	theme	LLPI	932:935	arg1	characteristics					913:927	the microbial degradation characteristics	887:927	the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum	887:988	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	1	75	dep	lotus	226:230	arg1	leaves					232:237	leaves	232:237	leaves	232:237	Polysaccharides exist as one of the most abundant components in lotus leaves, which attract increasing attention owing to their promising health-promoting benefits.
35520274	6	76	theme	galactose	1291:1299	arg1	ratios					1264:1269	the molar ratios	1254:1269	the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI	1254:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	6	77	theme	intestinal	1502:1511	arg1	microbiota					1513:1522	intestinal microbiota	1502:1522	intestinal microbiota	1502:1522	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	6	78	theme	fermentation	1586:1597	arg1	stage					1599:1603	the initial fermentation stage	1574:1603	the initial fermentation stage	1574:1603	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	8	79	theme	health	2162:2167	arg1	improvement					2136:2146	the improvement	2132:2146	the improvement of intestinal health	2132:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	5	80	theme	reducing	1044:1051	arg1	sugars					1053:1058	reducing sugars	1044:1058	reducing sugars released from LLPI	1044:1077	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	3	81	theme	different	627:635	arg1	stages					657:662	different simulated digestion stages	627:662	different simulated digestion stages	627:662	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	4	82	theme	microbial	891:899	arg1	characteristics					913:927	the microbial degradation characteristics	887:927	the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum	887:988	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	6	83	theme	galactose	1481:1489	arg1	faster					1533:1538	faster	1533:1538	faster	1533:1538	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	6	83	theme	galactose	1481:1489	arg1	utilization					1452:1462	the utilization	1448:1462	the utilization of arabinose and galactose in LLPI by intestinal microbiota	1448:1522	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	7	84	theme	microbial	1655:1663	arg1	composition					1665:1675	gut microbial composition	1651:1675	gut microbial composition	1651:1675	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	4	85	theme	human	969:973	arg1	inoculum					981:988	human fecal inoculum	969:988	human fecal inoculum	969:988	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	0	86	from	characteristics	19:33	arg1	lotus					85:89	lotus leaves	85:96	lotus leaves	85:96	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	0	86	from	characteristics	19:33	arg1	effects					110:116	related effects	102:116	related effects on the modulation of intestinal microbiota	102:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	7	87	theme	acetic	1890:1895	arg1	acids					1872:1876	several short-chain fatty acids	1846:1876	several short-chain fatty acids	1846:1876	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	87	theme	acetic	1890:1895	arg1	acids					1921:1925	acetic, propionic, and butyric acids	1890:1925	acetic, propionic, and butyric acids	1890:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	6	88	theme	arabinose	1467:1475	arg1	faster					1533:1538	faster	1533:1538	faster	1533:1538	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	6	88	theme	arabinose	1467:1475	arg1	utilization					1452:1462	the utilization	1448:1462	the utilization of arabinose and galactose in LLPI by intestinal microbiota	1448:1522	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	0	89	theme	related	102:108	arg1	effects					110:116	related effects	102:116	related effects on the modulation of intestinal microbiota	102:159	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	5	90	theme	fermentation	1140:1151	arg1	0-6 h					1160:1164	0-6 h	1160:1164	0-6 h	1160:1164	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	5	90	theme	fermentation	1140:1151	arg1	stage					1153:1157	the initial fermentation stage	1128:1157	the initial fermentation stage (0-6 h)	1128:1165	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	6	91	theme	galacturonic	1553:1564	arg1	acid					1566:1569	galacturonic acid	1553:1569	galacturonic acid at the initial fermentation stage	1553:1603	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	4	92	theme	in	944:945	arg1	fermentation					953:964	in vitro fermentation	944:964	in vitro fermentation by human fecal inoculum	944:988	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	6	93	theme	galacturonic	1312:1323	arg1	acid					1325:1328	galacturonic acid	1312:1328	galacturonic acid	1312:1328	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	7	94	theme	fatty	1866:1870	arg1	acids					1872:1876	several short-chain fatty acids	1846:1876	several short-chain fatty acids	1846:1876	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	94	theme	fatty	1866:1870	arg1	acids					1921:1925	acetic, propionic, and butyric acids	1890:1925	acetic, propionic, and butyric acids	1890:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	4	95	theme	in	781:782	arg1	fermentation					790:801	in vitro fermentation	781:801	in vitro fermentation	781:801	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	2	96	theme	leaf	407:410	arg1	LLP					429:431	LLP	429:431	LLP	429:431	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	2	96	theme	leaf	407:410	arg1	polysaccharides					412:426	lotus leaf polysaccharides	401:426	lotus leaf polysaccharides (LLP)	401:432	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	6	97	from	acid	1325:1328	arg1	LLPI					1340:1343	LLPI	1340:1343	LLPI	1340:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	7	98	theme	several	1846:1852	arg1	acids					1872:1876	several short-chain fatty acids	1846:1876	several short-chain fatty acids	1846:1876	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	7	98	theme	several	1846:1852	arg1	acids					1921:1925	acetic, propionic, and butyric acids	1890:1925	acetic, propionic, and butyric acids	1890:1925	Additionally, LLPI could remarkably regulate gut microbial composition by increasing the abundances of several beneficial microbes, including Bacteroides, Bifidobacterium, Megamonas, and Collinsella, resulting in the promoted generation of several short-chain fatty acids, especially acetic, propionic, and butyric acids.
35520274	6	99	from	stage	1599:1603	arg1	acid					1566:1569	galacturonic acid	1553:1569	galacturonic acid at the initial fermentation stage	1553:1603	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	4	100	theme	intestinal	737:746	arg1	microbiota					748:757	intestinal microbiota	737:757	intestinal microbiota in human feces	737:772	Conversely, the indigestible LLP (LLPI) could be remarkably utilized by intestinal microbiota in human feces during in vitro fermentation, and its fermentability was 58.11% after the in vitro fermentation of 48 h. Indeed, the microbial degradation characteristics of LLPI during in vitro fermentation by human fecal inoculum were revealed.
35520274	8	101	theme	degradation	2031:2041	arg1	characteristics					2043:2057	the digestive and microbial degradation characteristics	2003:2057	the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health	2003:2167	The findings from the present study are beneficial to better understanding the digestive and microbial degradation characteristics of LLP, which indicate that LLP can be used as a potential prebiotic for the improvement of intestinal health.
35520274	0	102	theme	microbial	39:47	arg1	degradation					49:59	microbial degradation	39:59	microbial degradation	39:59	In vitro digestive characteristics and microbial degradation of polysaccharides from lotus leaves and related effects on the modulation of intestinal microbiota.
35520274	5	103	theme	molecular	1176:1184	arg1	weight					1186:1191	its molecular weight	1172:1191	its molecular weight	1172:1191	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	5	104	attach	released	1060:1067	arg1	LLPI					1074:1077	LLPI	1074:1077	LLPI	1074:1077	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	5	104	attach	released	1060:1067	arg2	sugars					1053:1058	reducing sugars	1044:1058	reducing sugars released from LLPI	1044:1077	The results showed that the content of reducing sugars released from LLPI obviously increased from 0.498 to 2.176 mg/mL at the initial fermentation stage (0-6 h), and its molecular weight sharply decreased from 4.08 × 104 to 2.02 × 104 Da.
35520274	2	105	theme	gastrointestinal	468:483	arg1	model					485:489	an in vitro gastrointestinal model	456:489	an in vitro gastrointestinal model	456:489	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	3	106	theme	digestion	647:655	arg1	stages					657:662	different simulated digestion stages	627:662	different simulated digestion stages	627:662	The results suggested that LLP was stable in the human upper gastrointestinal tract in vitro according to its digestive stabilities at different simulated digestion stages.
35520274	2	107	theme	in	459:460	arg1	model					485:489	an in vitro gastrointestinal model	456:489	an in vitro gastrointestinal model	456:489	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	6	108	from	ratios	1264:1269	arg1	LLPI					1340:1343	LLPI	1340:1343	LLPI	1340:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35520274	2	109	theme	degradation	370:380	arg1	characteristics					382:396	the digestive and microbial degradation characteristics	342:396	the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP)	342:432	In this study, the digestive and microbial degradation characteristics of lotus leaf polysaccharides (LLP) were studied by using an in vitro gastrointestinal model.
35520274	6	110	from	arabinose	1274:1282	arg1	LLPI					1340:1343	LLPI	1340:1343	LLPI	1340:1343	Notably, the molar ratios of arabinose (Ara), galactose (Gal), and galacturonic acid (GalA) in LLPI decreased from 2.89 to 1.40, from 5.46 to 3.72, and from 21.24 to 18.71, respectively, suggesting that the utilization of arabinose and galactose in LLPI by intestinal microbiota was much faster than that of galacturonic acid at the initial fermentation stage.
35054662	4	0	theme	O-2-β-d-mannosyl-	945:961	arg1	-α-d-galactan					967:979	a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan	934:979	a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan	934:979	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	0	1	from	Basidiocarps	21:32	arg1	Isolation					79:87	Isolation	79:87	Isolation	79:87	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	0	1	from	Basidiocarps	21:32	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.	0:102	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	0	1	from	Basidiocarps	21:32	arg1	Structure					93:101	Structure	93:101	Structure	93:101	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	1	2	from	characterization	162:177	arg1	basidiocarp					205:215	a basidiocarp	203:215	a basidiocarp of polypore fungus Ganoderma resinaceum	203:255	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	6	3	theme	original	1391:1398	arg1	fraction					1400:1407	the original fraction	1387:1407	the original fraction	1387:1407	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	7	4	theme	alkaline	1414:1421	arg1	extract					1423:1429	The alkaline extract	1410:1429	The alkaline extract	1410:1429	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	2	5	theme	hydroxide	436:444	arg1	solution					407:414	a solution	405:414	a solution of 1 mol L-1 sodium hydroxide	405:444	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	5	theme	hydroxide	436:444	arg1	water					372:376	hot water	368:376	hot water under reflux (100 °C)	368:398	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	5	theme	hydroxide	436:444	arg1	water					333:337	cold water	328:337	cold water at room temperature (20 °C)	328:365	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	7	6	theme	terminal	1513:1520	arg1	residues					1535:1542	terminal β-d-glucosyl residues	1513:1542	terminal β-d-glucosyl residues attached to O-6 of the backbone	1513:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	7	7	dep	branched	1480:1487	arg1	1→3					1490:1492	1→3	1490:1492	1→3	1490:1492	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	1	8	from	isolation	137:145	arg1	basidiocarp					205:215	a basidiocarp	203:215	a basidiocarp of polypore fungus Ganoderma resinaceum	203:255	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	3	9	theme	nuclear	817:823	arg1	NMR					845:847	NMR	845:847	NMR	845:847	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	9	theme	nuclear	817:823	arg1	resonance					834:842	correlation nuclear magnetic resonance	805:842	correlation nuclear magnetic resonance (NMR) spectroscopy	805:861	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	4	10	theme	main	899:902	arg1	polysaccharides					904:918	two main polysaccharides	895:918	two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan	895:1028	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	5	11	theme	cold	1065:1068	arg1	extract					1076:1082	the cold water extract	1061:1082	the cold water extract	1061:1082	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	6	12	theme	similar	1340:1346	arg1	structure					1330:1338	a structure	1328:1338	a structure similar to the corresponding polysaccharide of the original fraction	1328:1407	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	7	13	attach	attached	1544:1551	arg1	O-6					1556:1558	O-6	1556:1558	O-6 of the backbone	1556:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	7	13	attach	attached	1544:1551	arg2	residues					1535:1542	terminal β-d-glucosyl residues	1513:1542	terminal β-d-glucosyl residues attached to O-6 of the backbone	1513:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	3	14	theme	magnetic	825:832	arg1	NMR					845:847	NMR	845:847	NMR	845:847	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	14	theme	magnetic	825:832	arg1	resonance					834:842	correlation nuclear magnetic resonance	805:842	correlation nuclear magnetic resonance (NMR) spectroscopy	805:861	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	4	15	theme	branched	936:943	arg1	-α-d-galactan					967:979	a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan	934:979	a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan	934:979	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	6	16	theme	them	1278:1281	arg1	them					1278:1281	them	1278:1281	them	1278:1281	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	6	16	theme	them	1278:1281	arg1	two					1271:1273	two	1271:1273	two	1271:1273	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	6	16	theme	them	1278:1281	arg1	β-d-glucans					1311:1321	branched β-d-glucans	1302:1321	branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction	1302:1407	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	2	17	theme	successive	300:309	arg1	extractions					311:321	successive extractions	300:321	successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide	300:444	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	3	18	dep	Fourier	500:506	arg1	transform					508:516	transform	508:516	transform infrared (FTIR) spectroscopy	508:545	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	4	19	dep	branched	994:1001	arg1	1→3					1004:1006	1→3	1004:1006	1→3	1004:1006	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	4	19	dep	branched	994:1001	arg1	1→4					1009:1011	1→4	1009:1011	1→4	1009:1011	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	4	19	dep	branched	994:1001	arg1	1→6					1014:1016	1→6	1014:1016	1→6	1014:1016	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	5	20	theme	hot	1128:1130	arg1	extract					1138:1144	the hot water extract	1124:1144	the hot water extract	1124:1144	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	8	21	theme	polysaccharide	1638:1651	arg1	complex					1653:1659	a polysaccharide complex	1636:1659	a polysaccharide complex containing chitin and β-d-glucans	1636:1693	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	8	21	theme	polysaccharide	1638:1651	arg1	part					1591:1594	The insoluble part	1577:1594	The insoluble part after all extractions	1577:1616	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	6	22	theme	preparative	1203:1213	arg1	chromatography					1230:1243	preparative anion exchange chromatography	1203:1243	preparative anion exchange chromatography	1203:1243	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	1	23	theme	structural	151:160	arg1	characterization					162:177	structural characterization	151:177	structural characterization	151:177	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	2	24	theme	1	419:419	arg1	mol					421:423	mol	421:423	mol	421:423	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	0	25	theme	Polypore	41:48	arg1	resinaceum					67:76	the Polypore Fungus Ganoderma resinaceum	37:76	the Polypore Fungus Ganoderma resinaceum	37:76	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	7	26	theme	branched	1480:1487	arg1	-β-d-glucan					1494:1504	a weakly branched (1→3)-β-d-glucan	1471:1504	a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone	1471:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	5	27	theme	extract	1138:1144	arg1	product					1113:1119	the main product	1104:1119	the main product of the hot water extract	1104:1144	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	5	27	theme	extract	1138:1144	arg1	β-d-glucan					1089:1098	β-d-glucan	1089:1098	β-d-glucan	1089:1098	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	5	28	theme	water	1132:1136	arg1	extract					1138:1144	the hot water extract	1124:1144	the hot water extract	1124:1144	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	7	29	contain	contained	1431:1439	arg2	-β-d-glucan					1494:1504	a weakly branched (1→3)-β-d-glucan	1471:1504	a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone	1471:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	7	29	contain	contained	1431:1439	arg2	1→3					1451:1453	1→3	1451:1453	1→3	1451:1453	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	7	29	contain	contained	1431:1439	arg2	-α-d-glucan					1455:1465	a linear (1→3)-α-d-glucan	1441:1465	a linear (1→3)-α-d-glucan	1441:1465	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	7	29	contain	contained	1431:1439	arg1	extract					1423:1429	The alkaline extract	1410:1429	The alkaline extract	1410:1429	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	3	30	theme	gas	676:678	arg1	chromatography					680:693	gas chromatography	676:693	gas chromatography	676:693	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	6	31	theme	water	1155:1159	arg1	fraction					1169:1176	The hot water soluble fraction	1147:1176	The hot water soluble fraction	1147:1176	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	1	32	dep	isolation	137:145	arg1	the					133:135	the	133:135	the	133:135	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	6	33	theme	corresponding	1355:1367	arg1	polysaccharide					1369:1382	the corresponding polysaccharide	1351:1382	the corresponding polysaccharide of the original fraction	1351:1407	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	3	34	theme	ionization	715:724	arg1	GC/FID					736:741	GC/FID	736:741	GC/FID	736:741	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	34	theme	ionization	715:724	arg1	detector					726:733	flame ionization detector	709:733	flame ionization detector (GC/FID)	709:742	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	6	35	theme	hot	1151:1153	arg1	fraction					1169:1176	The hot water soluble fraction	1147:1176	The hot water soluble fraction	1147:1176	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	8	36	contain	containing	1661:1670	arg2	chitin					1672:1677	chitin	1672:1677	chitin	1672:1677	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	8	36	contain	containing	1661:1670	arg1	complex					1653:1659	a polysaccharide complex	1636:1659	a polysaccharide complex containing chitin and β-d-glucans	1636:1693	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	8	36	contain	containing	1661:1670	arg1	part					1591:1594	The insoluble part	1577:1594	The insoluble part after all extractions	1577:1616	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	8	36	contain	containing	1661:1670	arg2	β-d-glucans					1683:1693	β-d-glucans	1683:1693	β-d-glucans	1683:1693	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	1	37	theme	polysaccharides	182:196	arg1	isolation					137:145	isolation	137:145	isolation	137:145	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	1	37	theme	polysaccharides	182:196	arg1	characterization					162:177	structural characterization	151:177	structural characterization	151:177	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	4	38	theme	branched	994:1001	arg1	-β-d-glucan					1018:1028	a highly branched (1→3)(1→4)(1→6)-β-d-glucan	985:1028	a highly branched (1→3)(1→4)(1→6)-β-d-glucan	985:1028	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	3	39	theme	mass	748:751	arg1	detector					766:773	mass spectrometry detector	748:773	mass spectrometry detector (GC/MS)	748:781	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	39	theme	mass	748:751	arg1	GC/MS					776:780	GC/MS	776:780	GC/MS	776:780	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	4	40	theme	aqueous	868:874	arg1	extracts					876:883	The aqueous extracts	864:883	The aqueous extracts	864:883	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	2	41	theme	cold	328:331	arg1	water					333:337	cold water	328:337	cold water at room temperature (20 °C)	328:365	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	42	from	temperature	347:357	arg1	solution					407:414	a solution	405:414	a solution of 1 mol L-1 sodium hydroxide	405:444	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	42	from	temperature	347:357	arg1	water					372:376	hot water	368:376	hot water under reflux (100 °C)	368:398	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	42	from	temperature	347:357	arg1	water					333:337	cold water	328:337	cold water at room temperature (20 °C)	328:365	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	3	43	theme	infrared	518:525	arg1	spectroscopy					534:545	infrared (FTIR) spectroscopy	518:545	infrared (FTIR) spectroscopy	518:545	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	7	44	theme	backbone	1567:1574	arg1	O-6					1556:1558	O-6	1556:1558	O-6 of the backbone	1556:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	3	45	theme	fractions	465:473	arg1	purity					451:456	The purity	447:456	The purity of all fractions	447:473	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	2	46	theme	sodium	429:434	arg1	hydroxide					436:444	1 mol L-1 sodium hydroxide	419:444	1 mol L-1 sodium hydroxide	419:444	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	3	47	theme	flame	709:713	arg1	GC/FID					736:741	GC/FID	736:741	GC/FID	736:741	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	47	theme	flame	709:713	arg1	detector					726:733	flame ionization detector	709:733	flame ionization detector (GC/FID)	709:742	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	6	48	theme	branched	1302:1309	arg1	them					1278:1281	them	1278:1281	them	1278:1281	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	6	48	theme	branched	1302:1309	arg1	two					1271:1273	two	1271:1273	two	1271:1273	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	6	48	theme	branched	1302:1309	arg1	β-d-glucans					1311:1321	branched β-d-glucans	1302:1321	branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction	1302:1407	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	3	49	theme	organic	606:612	arg1	analysis					624:631	organic elemental analysis	606:631	organic elemental analysis	606:631	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	2	50	theme	L-1	425:427	arg1	hydroxide					436:444	1 mol L-1 sodium hydroxide	419:444	1 mol L-1 sodium hydroxide	419:444	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	5	51	theme	water	1070:1074	arg1	extract					1076:1082	the cold water extract	1061:1082	the cold water extract	1061:1082	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	2	52	with	extractions	311:321	arg1	solution					407:414	a solution	405:414	a solution of 1 mol L-1 sodium hydroxide	405:444	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	52	with	extractions	311:321	arg1	water					372:376	hot water	368:376	hot water under reflux (100 °C)	368:398	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	52	with	extractions	311:321	arg1	water					333:337	cold water	328:337	cold water at room temperature (20 °C)	328:365	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	1	53	from	basidiocarp	205:215	arg1	polysaccharides					182:196	polysaccharides	182:196	polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum	182:255	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	1	53	from	basidiocarp	205:215	arg1	isolation					137:145	isolation	137:145	isolation	137:145	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	1	53	from	basidiocarp	205:215	arg1	characterization					162:177	structural characterization	151:177	structural characterization	151:177	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	3	54	theme	spectrometry	753:764	arg1	detector					766:773	mass spectrometry detector	748:773	mass spectrometry detector (GC/MS)	748:781	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	54	theme	spectrometry	753:764	arg1	GC/MS					776:780	GC/MS	776:780	GC/MS	776:780	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	0	55	theme	Ganoderma	57:65	arg1	resinaceum					67:76	the Polypore Fungus Ganoderma resinaceum	37:76	the Polypore Fungus Ganoderma resinaceum	37:76	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	4	56	contain	contained	885:893	arg1	extracts					876:883	The aqueous extracts	864:883	The aqueous extracts	864:883	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	4	56	contain	contained	885:893	arg2	polysaccharides					904:918	two main polysaccharides	895:918	two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan	895:1028	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	0	57	theme	Fungus	50:55	arg1	resinaceum					67:76	the Polypore Fungus Ganoderma resinaceum	37:76	the Polypore Fungus Ganoderma resinaceum	37:76	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	5	58	theme	main	1108:1111	arg1	product					1113:1119	the main product	1104:1119	the main product of the hot water extract	1104:1144	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	5	58	theme	main	1108:1111	arg1	β-d-glucan					1089:1098	β-d-glucan	1089:1098	β-d-glucan	1089:1098	Mannogalactan predominated in the cold water extract, and β-d-glucan was the main product of the hot water extract.
35054662	6	59	theme	exchange	1221:1228	arg1	chromatography					1230:1243	preparative anion exchange chromatography	1203:1243	preparative anion exchange chromatography	1203:1243	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	7	60	theme	linear	1443:1448	arg1	-α-d-glucan					1455:1465	a linear (1→3)-α-d-glucan	1441:1465	a linear (1→3)-α-d-glucan	1441:1465	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	7	60	theme	linear	1443:1448	arg1	1→3					1451:1453	1→3	1451:1453	1→3	1451:1453	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	6	61	theme	soluble	1161:1167	arg1	fraction					1169:1176	The hot water soluble fraction	1147:1176	The hot water soluble fraction	1147:1176	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	6	62	with	β-d-glucans	1311:1321	arg1	structure					1330:1338	a structure	1328:1338	a structure similar to the corresponding polysaccharide of the original fraction	1328:1407	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	7	63	theme	β-d-glucosyl	1522:1533	arg1	residues					1535:1542	terminal β-d-glucosyl residues	1513:1542	terminal β-d-glucosyl residues attached to O-6 of the backbone	1513:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	6	64	theme	anion	1215:1219	arg1	chromatography					1230:1243	preparative anion exchange chromatography	1203:1243	preparative anion exchange chromatography	1203:1243	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	3	65	theme	neutral	634:640	arg1	sugar					642:646	neutral sugar and methylation analyses	634:671	sugar	642:646	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	66	theme	correlation	805:815	arg1	NMR					845:847	NMR	845:847	NMR	845:847	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	66	theme	correlation	805:815	arg1	resonance					834:842	correlation nuclear magnetic resonance	805:842	correlation nuclear magnetic resonance (NMR) spectroscopy	805:861	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	1	67	theme	polypore	220:227	arg1	resinaceum					246:255	polypore fungus Ganoderma resinaceum	220:255	polypore fungus Ganoderma resinaceum	220:255	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	4	68	theme	1→6	963:965	arg1	-α-d-galactan					967:979	a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan	934:979	a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan	934:979	The aqueous extracts contained two main polysaccharides identified as a branched O-2-β-d-mannosyl-(1→6)-α-d-galactan and a highly branched (1→3)(1→4)(1→6)-β-d-glucan.
35054662	3	69	theme	elemental	614:622	arg1	analysis					624:631	organic elemental analysis	606:631	organic elemental analysis	606:631	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	2	70	theme	room	342:345	arg1	temperature					347:357	room temperature	342:357	room temperature (20 °C)	342:365	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	2	70	theme	room	342:345	arg1	°C					363:364	20 °C	360:364	20 °C	360:364	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	8	71	theme	insoluble	1581:1589	arg1	complex					1653:1659	a polysaccharide complex	1636:1659	a polysaccharide complex containing chitin and β-d-glucans	1636:1693	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	8	71	theme	insoluble	1581:1589	arg1	part					1591:1594	The insoluble part	1577:1594	The insoluble part after all extractions	1577:1616	The insoluble part after all extractions was identified as a polysaccharide complex containing chitin and β-d-glucans.
35054662	1	72	theme	fungus	229:234	arg1	resinaceum					246:255	polypore fungus Ganoderma resinaceum	220:255	polypore fungus Ganoderma resinaceum	220:255	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	7	73	contain	having	1506:1511	arg1	-β-d-glucan					1494:1504	a weakly branched (1→3)-β-d-glucan	1471:1504	a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone	1471:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	7	73	contain	having	1506:1511	arg2	residues					1535:1542	terminal β-d-glucosyl residues	1513:1542	terminal β-d-glucosyl residues attached to O-6 of the backbone	1513:1574	The alkaline extract contained a linear (1→3)-α-d-glucan and a weakly branched (1→3)-β-d-glucan having terminal β-d-glucosyl residues attached to O-6 of the backbone.
35054662	2	74	theme	Polysaccharide	258:271	arg1	fractions					273:281	Polysaccharide fractions	258:281	Polysaccharide fractions	258:281	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	6	75	theme	fraction	1400:1407	arg1	polysaccharide					1369:1382	the corresponding polysaccharide	1351:1382	the corresponding polysaccharide of the original fraction	1351:1407	The hot water soluble fraction was further separated by preparative anion exchange chromatography into three sub-fractions; two of them were identified as branched β-d-glucans with a structure similar to the corresponding polysaccharide of the original fraction.
35054662	2	76	theme	hot	368:370	arg1	water					372:376	hot water	368:376	hot water under reflux (100 °C)	368:398	Polysaccharide fractions were obtained by successive extractions with cold water at room temperature (20 °C), hot water under reflux (100 °C), and a solution of 1 mol L-1 sodium hydroxide.
35054662	1	77	theme	Ganoderma	236:244	arg1	resinaceum					246:255	polypore fungus Ganoderma resinaceum	220:255	polypore fungus Ganoderma resinaceum	220:255	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
35054662	0	78	theme	resinaceum	67:76	arg1	Basidiocarps					21:32	Basidiocarps	21:32	Basidiocarps of the Polypore Fungus Ganoderma resinaceum	21:76	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	3	79	theme	methylation	652:662	arg1	analyses					664:671	neutral sugar and methylation analyses	634:671	analyses	664:671	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	0	80	dep	Polysaccharides	0:14	arg1	Isolation					79:87	Isolation	79:87	Isolation	79:87	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	0	80	dep	Polysaccharides	0:14	arg1	Polysaccharides					0:14	Polysaccharides	0:14	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.	0:102	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	0	80	dep	Polysaccharides	0:14	arg1	Structure					93:101	Structure	93:101	Structure	93:101	Polysaccharides from Basidiocarps of the Polypore Fungus Ganoderma resinaceum: Isolation and Structure.
35054662	3	81	dep	infrared	518:525	arg1	FTIR					528:531	FTIR	528:531	FTIR	528:531	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	3	82	theme	resonance	834:842	arg1	spectroscopy					850:861	correlation nuclear magnetic resonance (NMR) spectroscopy	805:861	correlation nuclear magnetic resonance (NMR) spectroscopy	805:861	The purity of all fractions was controlled mainly by Fourier transform infrared (FTIR) spectroscopy, and their composition and structure were characterized by organic elemental analysis; neutral sugar and methylation analyses by gas chromatography equipped with flame ionization detector (GC/FID) and mass spectrometry detector (GC/MS), respectively; and by correlation nuclear magnetic resonance (NMR) spectroscopy.
35054662	1	83	theme	resinaceum	246:255	arg1	basidiocarp					205:215	a basidiocarp	203:215	a basidiocarp of polypore fungus Ganoderma resinaceum	203:255	In this study, we focused on the isolation and structural characterization of polysaccharides from a basidiocarp of polypore fungus Ganoderma resinaceum.
36295049	11	0	theme	food	1753:1756	arg1	supplements					1758:1768	biologically active food supplements	1733:1768	biologically active food supplements	1733:1768	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	4	1	theme	extractant	671:680	arg1	type					682:685	extractant type	671:685	extractant type	671:685	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	4	1	theme	extractant	671:680	arg1	parameters					636:645	cultivation parameters	624:645	cultivation parameters (temperature, time, and extractant type)	624:686	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	3	2	from	effect	434:439	arg1	growth					479:484	the growth	475:484	the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae	475:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	2	3	theme	culture	354:360	arg1	medium					362:367	the culture medium	350:367	the culture medium	350:367	Among them, endopolysaccharides (accumulated in cells) and exopolysaccharides (released by cells into the culture medium) are of particular interest.
36295049	6	4	theme	culture	979:985	arg1	medium					987:992	the culture medium	975:992	the culture medium	975:992	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	7	5	from	increase	1150:1157	arg1	punctata					1169:1176	the V. punctata biomass	1162:1184	the V. punctata biomass when cultivated on different nutrient media	1162:1228	It was found that the increase in the V. punctata biomass when cultivated on different nutrient media did not differ significantly.
36295049	3	6	theme	punctata	492:499	arg1	growth					479:484	the growth	475:484	the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae	475:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	6	7	theme	polysaccharides	956:970	arg1	higher					1026:1031	higher	1026:1031	higher	1026:1031	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	6	7	theme	polysaccharides	956:970	arg1	concentration					939:951	the concentration	935:951	the concentration of polysaccharides in the culture medium	935:992	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	0	8	theme	Cultivation	90:100	arg1	Conditions					102:111	Cultivation Conditions	90:111	Cultivation Conditions	90:111	Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions.
36295049	8	9	theme	1.101	1317:1321	arg1	mg/g					1323:1326	mg/g	1323:1326	mg/g	1323:1326	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	4	10	theme	V.	750:751	arg1	punctate					753:760	the microalgae V. punctate	735:760	the microalgae V. punctate	735:760	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	8	11	from	values	1280:1285	arg1	media					1306:1310	BBM 3N media	1299:1310	BBM 3N media	1299:1310	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	8	11	from	values	1280:1285	arg1	Prat					1290:1293	Prat	1290:1293	Prat	1290:1293	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	1	12	contain	has	165:167	arg1	microalga					150:158	a unicellular microalga	136:158	a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties	136:245	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	1	12	contain	has	165:167	arg1	punctata					124:131	Vischeria punctata	114:131	Vischeria punctata	114:131	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	1	12	contain	has	165:167	arg2	potential					180:188	industrial potential	169:188	industrial potential	169:188	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	3	13	theme	polysaccharides	530:544	arg1	punctata					492:499	V. punctata biomass and the synthesis of polysaccharides by microalgae	489:558	V. punctata biomass and the synthesis of polysaccharides by microalgae	489:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	3	13	theme	polysaccharides	530:544	arg1	biomass					501:507	biomass	501:507	biomass	501:507	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	3	13	theme	polysaccharides	530:544	arg1	synthesis					517:525	the synthesis	513:525	the synthesis of polysaccharides by microalgae	513:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	3	14	theme	V.	489:490	arg1	punctata					492:499	V. punctata biomass and the synthesis of polysaccharides by microalgae	489:558	V. punctata biomass and the synthesis of polysaccharides by microalgae	489:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	3	14	theme	V.	489:490	arg1	biomass					501:507	biomass	501:507	biomass	501:507	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	3	14	theme	V.	489:490	arg1	synthesis					517:525	the synthesis	513:525	the synthesis of polysaccharides by microalgae	513:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	5	15	theme	precipitation	849:861	arg1	methods					784:790	The methods	780:790	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation	780:861	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation were used in the study.
36295049	8	16	theme	biomass	1272:1278	arg1	d.w.					1328:1331	d.w.	1328:1331	d.w.	1328:1331	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	8	16	theme	biomass	1272:1278	arg1	values					1280:1285	The maximum biomass values	1260:1285	The maximum biomass values on Prat and BBM 3N media	1260:1310	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	1	17	theme	industrial	169:178	arg1	potential					180:188	industrial potential	169:188	industrial potential	169:188	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	4	18	from	effect	565:570	arg1	yield					695:699	the yield	691:699	the yield of exopolysaccharides produced by the microalgae V. punctate	691:760	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	10	19	theme	V.	1556:1557	arg1	punctata					1559:1566	V. punctata	1556:1566	V. punctata	1556:1566	It follows on from the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata.
36295049	5	20	theme	extraction	825:834	arg1	methods					784:790	The methods	780:790	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation	780:861	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation were used in the study.
36295049	4	21	theme	standard	587:594	arg1	medium					605:610	a standard nutrient medium	585:610	a standard nutrient medium	585:610	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	2	22	theme	particular	377:386	arg1	interest					388:395	particular interest	377:395	particular interest	377:395	Among them, endopolysaccharides (accumulated in cells) and exopolysaccharides (released by cells into the culture medium) are of particular interest.
36295049	10	23	theme	modified	1479:1486	arg1	medium					1494:1499	the modified PratM medium	1475:1499	the modified PratM medium	1475:1499	It follows on from the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata.
36295049	10	23	theme	modified	1479:1486	arg1	efficient					1510:1518	efficient	1510:1518	efficient	1510:1518	It follows on from the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata.
36295049	6	24	theme	BBM	1103:1105	arg1	3N					1107:1108	BBM 3N	1103:1108	BBM 3N (63.0 mg/g d.w.)	1103:1125	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	6	24	theme	BBM	1103:1105	arg1	mg/g					1116:1119	63.0 mg/g d.w.	1111:1124	63.0 mg/g d.w.	1111:1124	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	7	25	theme	biomass	1178:1184	arg1	punctata					1169:1176	the V. punctata biomass	1162:1184	the V. punctata biomass when cultivated on different nutrient media	1162:1228	It was found that the increase in the V. punctata biomass when cultivated on different nutrient media did not differ significantly.
36295049	2	26	dep	endopolysaccharides	260:278	arg1	accumulated					281:291	accumulated	281:291	accumulated in cells	281:300	Among them, endopolysaccharides (accumulated in cells) and exopolysaccharides (released by cells into the culture medium) are of particular interest.
36295049	5	27	theme	spectrophotometry	795:811	arg1	methods					784:790	The methods	780:790	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation	780:861	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation were used in the study.
36295049	11	28	theme	new	1600:1602	arg1	sources					1604:1610	new sources	1600:1610	new sources of valuable chemicals (polysaccharides)	1600:1650	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	3	29	dep	punctata	492:499	arg1	punctata					492:499	V. punctata biomass and the synthesis of polysaccharides by microalgae	489:558	V. punctata biomass and the synthesis of polysaccharides by microalgae	489:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	3	29	dep	punctata	492:499	arg1	biomass					501:507	biomass	501:507	biomass	501:507	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	3	29	dep	punctata	492:499	arg1	synthesis					517:525	the synthesis	513:525	the synthesis of polysaccharides by microalgae	513:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	11	30	theme	microalgae	1586:1595	arg1	potential					1573:1581	The potential	1569:1581	The potential	1569:1581	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	0	31	theme	Polysaccharide	13:26	arg1	Production					28:37	the Polysaccharide Production	9:37	the Polysaccharide Production	9:37	Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions.
36295049	11	32	theme	developing	1698:1707	arg1	foods					1726:1730	developing novel functional foods	1698:1730	developing novel functional foods	1698:1730	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	3	33	theme	medium	453:458	arg1	composition					460:470	nutrient medium composition	444:470	nutrient medium composition	444:470	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	4	34	theme	exopolysaccharides	704:721	arg1	yield					695:699	the yield	691:699	the yield of exopolysaccharides produced by the microalgae V. punctate	691:760	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	6	35	dep	higher	1026:1031	arg1	p					1034:1034	p	1034:1034	p &lt;0.05	1034:1043	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	1	36	with	substances	209:218	arg1	properties					236:245	beneficial properties	225:245	beneficial properties	225:245	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	11	37	used	used	1673:1676	arg2	potential					1573:1581	The potential	1569:1581	The potential	1569:1581	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	9	38	theme	culture	1418:1424	arg1	media					1426:1430	the culture media	1414:1430	the culture media	1414:1430	Neutral sugars and uronic acids were found in the culture media.
36295049	3	39	theme	composition	460:470	arg1	effect					434:439	the effect	430:439	the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae	430:558	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	7	40	theme	different	1205:1213	arg1	media					1224:1228	different nutrient media	1205:1228	different nutrient media	1205:1228	It was found that the increase in the V. punctata biomass when cultivated on different nutrient media did not differ significantly.
36295049	8	41	theme	1.120	1337:1341	arg1	mg/g					1343:1346	mg/g	1343:1346	mg/g	1343:1346	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	8	42	theme	3N	1303:1304	arg1	media					1306:1310	BBM 3N media	1299:1310	BBM 3N media	1299:1310	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	11	43	theme	pharmaceutical	1775:1788	arg1	substances					1790:1799	pharmaceutical substances	1775:1799	pharmaceutical substances	1775:1799	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	6	44	dep	mg/g	1116:1119	arg1	d.w.					1121:1124	d.w.	1121:1124	d.w.	1121:1124	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	0	45	theme	Production	28:37	arg1	Study					0:4	Study	0:4	Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions.	0:112	Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions.
36295049	6	46	theme	cultivation	922:932	arg1	days					914:917	61 days	911:917	61 days of cultivation	911:932	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	6	47	from	concentration	939:951	arg1	medium					987:992	the culture medium	975:992	the culture medium	975:992	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	0	48	from	punctata	66:73	arg1	Relation					78:85	Relation	78:85	Relation to Cultivation Conditions	78:111	Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions.
36295049	4	49	dep	parameters	636:645	arg1	type					682:685	extractant type	671:685	extractant type	671:685	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	4	49	dep	parameters	636:645	arg1	parameters					636:645	cultivation parameters	624:645	cultivation parameters (temperature, time, and extractant type)	624:686	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	4	49	dep	parameters	636:645	arg1	time					661:664	time	661:664	time	661:664	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	4	49	dep	parameters	636:645	arg1	temperature					648:658	temperature	648:658	temperature	648:658	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	3	50	theme	nutrient	444:451	arg1	composition					460:470	nutrient medium composition	444:470	nutrient medium composition	444:470	This study aimed to investigate the effect of nutrient medium composition on the growth of V. punctata biomass and the synthesis of polysaccharides by microalgae.
36295049	0	51	theme	Microalga	46:54	arg1	punctata					66:73	the Microalga Vischeria punctata	42:73	the Microalga Vischeria punctata in Relation to Cultivation Conditions	42:111	Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions.
36295049	10	52	theme	obtained	1456:1463	arg1	data					1465:1468	the obtained data	1452:1468	the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata	1452:1566	It follows on from the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata.
36295049	11	53	theme	novel	1709:1713	arg1	foods					1726:1730	developing novel functional foods	1698:1730	developing novel functional foods	1698:1730	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	4	54	theme	microalgae	739:748	arg1	punctate					753:760	the microalgae V. punctate	735:760	the microalgae V. punctate	735:760	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	9	55	theme	Neutral	1368:1374	arg1	sugars					1376:1381	Neutral sugars	1368:1381	Neutral sugars	1368:1381	Neutral sugars and uronic acids were found in the culture media.
36295049	6	56	theme	nutrient	1064:1071	arg1	medium					1073:1078	a Prat nutrient medium	1057:1078	a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.)	1057:1125	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	6	56	theme	nutrient	1064:1071	arg1	mg/g					1087:1090	984.9 mg/g d.w.	1081:1095	984.9 mg/g d.w.	1081:1095	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	1	57	theme	Vischeria	114:122	arg1	microalga					150:158	a unicellular microalga	136:158	a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties	136:245	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	1	57	theme	Vischeria	114:122	arg1	punctata					124:131	Vischeria punctata	114:131	Vischeria punctata	114:131	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	10	58	theme	PratM	1488:1492	arg1	medium					1494:1499	the modified PratM medium	1475:1499	the modified PratM medium	1475:1499	It follows on from the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata.
36295049	10	58	theme	PratM	1488:1492	arg1	efficient					1510:1518	efficient	1510:1518	efficient	1510:1518	It follows on from the obtained data that the modified PratM medium was more efficient for extracting polysaccharides from V. punctata.
36295049	9	59	located	found	1405:1409	arg2	sugars					1376:1381	Neutral sugars	1368:1381	Neutral sugars	1368:1381	Neutral sugars and uronic acids were found in the culture media.
36295049	9	59	located	found	1405:1409	arg1	media					1426:1430	the culture media	1414:1430	the culture media	1414:1430	Neutral sugars and uronic acids were found in the culture media.
36295049	9	59	located	found	1405:1409	arg2	acids					1394:1398	uronic acids	1387:1398	uronic acids	1387:1398	Neutral sugars and uronic acids were found in the culture media.
36295049	9	60	theme	uronic	1387:1392	arg1	acids					1394:1398	uronic acids	1387:1398	uronic acids	1387:1398	Neutral sugars and uronic acids were found in the culture media.
36295049	7	61	theme	V.	1166:1167	arg1	punctata					1169:1176	the V. punctata biomass	1162:1184	the V. punctata biomass when cultivated on different nutrient media	1162:1228	It was found that the increase in the V. punctata biomass when cultivated on different nutrient media did not differ significantly.
36295049	11	62	theme	active	1746:1751	arg1	supplements					1758:1768	biologically active food supplements	1733:1768	biologically active food supplements	1733:1768	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	8	63	theme	BBM	1299:1301	arg1	media					1306:1310	BBM 3N media	1299:1310	BBM 3N media	1299:1310	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	11	64	theme	functional	1715:1724	arg1	foods					1726:1730	developing novel functional foods	1698:1730	developing novel functional foods	1698:1730	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	4	65	theme	nutrient	596:603	arg1	medium					605:610	a standard nutrient medium	585:610	a standard nutrient medium	585:610	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	8	66	theme	maximum	1264:1270	arg1	d.w.					1328:1331	d.w.	1328:1331	d.w.	1328:1331	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	8	66	theme	maximum	1264:1270	arg1	values					1280:1285	The maximum biomass values	1260:1285	The maximum biomass values on Prat and BBM 3N media	1260:1310	The maximum biomass values on Prat and BBM 3N media were 1.101 mg/g d.w. and 1.120 mg/g d.w., respectively.
36295049	5	67	used	used	868:871	arg2	methods					784:790	The methods	780:790	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation	780:861	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation were used in the study.
36295049	2	68	dep	exopolysaccharides	307:324	arg1	released					327:334	released	327:334	released by cells into the culture medium	327:367	Among them, endopolysaccharides (accumulated in cells) and exopolysaccharides (released by cells into the culture medium) are of particular interest.
36295049	1	69	theme	beneficial	225:234	arg1	properties					236:245	beneficial properties	225:245	beneficial properties	225:245	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	5	70	theme	alcohol	841:847	arg1	precipitation					849:861	alcohol precipitation	841:861	alcohol precipitation	841:861	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation were used in the study.
36295049	6	71	dep	mg/g	1087:1090	arg1	d.w.					1092:1095	d.w.	1092:1095	d.w.	1092:1095	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	4	72	theme	cultivation	624:634	arg1	parameters					636:645	cultivation parameters	624:645	cultivation parameters (temperature, time, and extractant type)	624:686	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	4	72	theme	cultivation	624:634	arg1	type					682:685	extractant type	671:685	extractant type	671:685	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	4	72	theme	cultivation	624:634	arg1	time					661:664	time	661:664	time	661:664	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	4	72	theme	cultivation	624:634	arg1	temperature					648:658	temperature	648:658	temperature	648:658	The effect of modifying a standard nutrient medium and varying cultivation parameters (temperature, time, and extractant type) on the yield of exopolysaccharides produced by the microalgae V. punctate was investigated.
36295049	1	73	theme	unicellular	138:148	arg1	microalga					150:158	a unicellular microalga	136:158	a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties	136:245	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	1	73	theme	unicellular	138:148	arg1	punctata					124:131	Vischeria punctata	114:131	Vischeria punctata	114:131	Vischeria punctata is a unicellular microalga that has industrial potential, as it can produce substances with beneficial properties.
36295049	7	74	theme	nutrient	1215:1222	arg1	media					1224:1228	different nutrient media	1205:1228	different nutrient media	1205:1228	It was found that the increase in the V. punctata biomass when cultivated on different nutrient media did not differ significantly.
36295049	11	75	theme	valuable	1615:1622	arg1	polysaccharides					1635:1649	polysaccharides	1635:1649	polysaccharides	1635:1649	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	11	75	theme	valuable	1615:1622	arg1	chemicals					1624:1632	valuable chemicals	1615:1632	valuable chemicals (polysaccharides)	1615:1650	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
36295049	0	76	theme	Vischeria	56:64	arg1	punctata					66:73	the Microalga Vischeria punctata	42:73	the Microalga Vischeria punctata in Relation to Cultivation Conditions	42:111	Study of the Polysaccharide Production by the Microalga Vischeria punctata in Relation to Cultivation Conditions.
36295049	5	77	theme	ultrasonic	814:823	arg1	extraction					825:834	ultrasonic extraction	814:834	ultrasonic extraction	814:834	The methods of spectrophotometry, ultrasonic extraction, and alcohol precipitation were used in the study.
36295049	6	78	theme	Prat	1059:1062	arg1	medium					1073:1078	a Prat nutrient medium	1057:1078	a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.)	1057:1125	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	6	78	theme	Prat	1059:1062	arg1	mg/g					1087:1090	984.9 mg/g d.w.	1081:1095	984.9 mg/g d.w.	1081:1095	It was found that after 61 days of cultivation, the concentration of polysaccharides in the culture medium was statistically significantly higher (p &lt;0.05) when using a Prat nutrient medium (984.9 mg/g d.w.) than BBM 3N (63.0 mg/g d.w.).
36295049	11	79	theme	chemicals	1624:1632	arg1	sources					1604:1610	new sources	1600:1610	new sources of valuable chemicals (polysaccharides)	1600:1650	The potential of microalgae as new sources of valuable chemicals (polysaccharides), which can be widely used in technologies for developing novel functional foods, biologically active food supplements, and pharmaceutical substances, was studied.
35055106	3	0	theme	loss	673:676	arg1	effect					657:662	the effect	653:662	the effect of PARP7 loss on DSS-induced intestinal inflammation	653:715	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	4	1	theme	genes	843:847	arg1	scores					788:793	lower disease index scores	768:793	lower disease index scores	768:793	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	1	theme	genes	843:847	arg1	loss					762:765	less body weight loss	745:765	less body weight loss	745:765	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	1	theme	genes	843:847	arg1	expression					808:817	reduced expression	800:817	reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2,	800:933	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	7	2	theme	cells	1405:1409	arg1	recruitment					1383:1393	the recruitment	1379:1393	the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling	1379:1509	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	4	3	theme	body	750:753	arg1	loss					762:765	less body weight loss	745:765	less body weight loss	745:765	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	4	theme	motif	884:888	arg1	ligand					900:905	C-x-c motif chemokine ligand 1	878:907	C-x-c motif chemokine ligand 1 (Cxcl1)	878:915	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	4	theme	motif	884:888	arg1	Cxcl1					910:914	Cxcl1	910:914	Cxcl1	910:914	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	5	5	theme	gene	1080:1083	arg1	expression					1051:1060	the colonic expression	1039:1060	the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1039:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	4	6	theme	inflammation	830:841	arg1	genes					843:847	several inflammation genes	822:847	several inflammation genes	822:847	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	6	theme	inflammation	830:841	arg1	ligand					900:905	C-x-c motif chemokine ligand 1	878:907	C-x-c motif chemokine ligand 1 (Cxcl1)	878:915	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	6	theme	inflammation	830:841	arg1	IL-6					872:875	interleukin IL-6	860:875	interleukin IL-6	860:875	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	6	theme	inflammation	830:841	arg1	lipocalin-2					922:932	lipocalin-2	922:932	lipocalin-2	922:932	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	7	7	theme	AHR	1497:1499	arg1	signaling					1501:1509	AHR signaling	1497:1509	IFN-I signaling rather than AHR signaling	1469:1509	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	5	8	theme	colonic	1043:1049	arg1	expression					1051:1060	the colonic expression	1039:1060	the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1039:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	5	9	theme	cytochrome	1085:1094	arg1	Cyp1a1					1106:1111	Cyp1a1	1106:1111	Cyp1a1	1106:1111	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	5	9	theme	cytochrome	1085:1094	arg1	1A1					1101:1103	cytochrome P450 1A1	1085:1103	the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1065:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	5	10	theme	AHR	1069:1071	arg1	gene					1080:1083	the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1065:1112	the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1065:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	4	11	theme	less	745:748	arg1	loss					762:765	less body weight loss	745:765	less body weight loss	745:765	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	3	12	theme	IFN-I	553:557	arg1	signaling					559:567	AHR and IFN-I signaling	545:567	AHR and IFN-I signaling	545:567	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	0	13	theme	-Deficient	111:120	arg1	Mice					122:125	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice	34:125	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice	34:125	Reduced Colonic Mucosal Injury in 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice.
35055106	3	14	theme	intestinal	693:702	arg1	inflammation					704:715	DSS-induced intestinal inflammation	681:715	DSS-induced intestinal inflammation	681:715	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	3	15	theme	AHR	545:547	arg1	signaling					559:567	AHR and IFN-I signaling	545:567	AHR and IFN-I signaling	545:567	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	1	16	theme	poly-ADP-ribose	287:301	arg1	polymerase					303:312	poly-ADP-ribose polymerase 7	287:314	poly-ADP-ribose polymerase 7 (PARP7)	287:322	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	16	theme	poly-ADP-ribose	287:301	arg1	PARP7					317:321	PARP7	317:321	PARP7	317:321	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	2	17	theme	receptor	369:376	arg1	signaling					414:422	aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling	352:422	aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling	352:422	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	7	18	theme	PARP7	1259:1263	arg1	protein					1265:1271	PARP7 protein	1259:1271	PARP7 protein	1259:1271	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	3	19	theme	PARP7	518:522	arg1	expression					524:533	PARP7 expression	518:533	PARP7 expression	518:533	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	7	20	theme	protein	1265:1271	arg1	absence					1248:1254	the absence	1244:1254	the absence of PARP7 protein	1244:1271	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	4	21	theme	wild-type	954:962	arg1	mice					964:967	wild-type mice	954:967	wild-type mice	954:967	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	7	22	theme	immune	1398:1403	arg1	cells					1405:1409	immune cells	1398:1409	immune cells	1398:1409	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	2	23	theme	intestinal	463:472	arg1	homeostasis					474:484	intestinal homeostasis	463:484	intestinal homeostasis	463:484	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	0	24	dep	-Deficient	111:120	arg1	Polymerase					86:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	Reduced Colonic Mucosal Injury in 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice.
35055106	2	25	theme	interferon	395:404	arg1	signaling					414:422	aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling	352:422	aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling	352:422	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	0	26	theme	Colonic	8:14	arg1	Injury					24:29	Colonic Mucosal Injury	8:29	Colonic Mucosal Injury	8:29	Reduced Colonic Mucosal Injury in 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice.
35055106	3	27	theme	DSS-induced	681:691	arg1	inflammation					704:715	DSS-induced intestinal inflammation	681:715	DSS-induced intestinal inflammation	681:715	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	4	28	theme	several	822:828	arg1	genes					843:847	several inflammation genes	822:847	several inflammation genes	822:847	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	28	theme	several	822:828	arg1	ligand					900:905	C-x-c motif chemokine ligand 1	878:907	C-x-c motif chemokine ligand 1 (Cxcl1)	878:915	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	28	theme	several	822:828	arg1	IL-6					872:875	interleukin IL-6	860:875	interleukin IL-6	860:875	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	28	theme	several	822:828	arg1	lipocalin-2					922:932	lipocalin-2	922:932	lipocalin-2	922:932	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	29	theme	DSS-exposed	718:728	arg1	mice					736:739	DSS-exposed Parp7 mice	718:739	DSS-exposed Parp7 mice	718:739	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	1	30	theme	system	203:208	arg1	polymerases					144:154	Poly-ADP-ribose polymerases	128:154	Poly-ADP-ribose polymerases (PARPs)	128:162	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	30	theme	system	203:208	arg1	regulators					178:187	important regulators	168:187	important regulators	168:187	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	30	theme	system	203:208	arg1	polymerase					252:261	TCDD-inducible poly-ADP-ribose polymerase	221:261	TCDD-inducible poly-ADP-ribose polymerase (TIPARP)	221:270	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	7	31	theme	inflammation	1418:1429	arg1	due					1450:1452	due	1450:1452	due	1450:1452	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	7	31	theme	inflammation	1418:1429	arg1	site					1431:1434	the inflammation site	1414:1434	the inflammation site	1414:1434	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	5	32	dep	gene	1080:1083	arg1	Cyp1a1					1106:1111	Cyp1a1	1106:1111	Cyp1a1	1106:1111	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	5	32	dep	gene	1080:1083	arg1	1A1					1101:1103	cytochrome P450 1A1	1085:1103	the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1065:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	3	33	from	effect	657:662	arg1	inflammation					704:715	DSS-induced intestinal inflammation	681:715	DSS-induced intestinal inflammation	681:715	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	2	34	theme	type	388:391	arg1	interferon					395:404	type I interferon	388:404	type I interferon (IFN-I)	388:412	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	2	34	theme	type	388:391	arg1	IFN-I					407:411	IFN-I	407:411	IFN-I	407:411	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	6	35	theme	significant	1128:1138	arg1	differences					1140:1150	no significant differences	1125:1150	no significant differences in microbial composition	1125:1175	Moreover, no significant differences in microbial composition were observed between the genotypes.
35055106	4	36	theme	chemokine	890:898	arg1	ligand					900:905	C-x-c motif chemokine ligand 1	878:907	C-x-c motif chemokine ligand 1 (Cxcl1)	878:915	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	36	theme	chemokine	890:898	arg1	Cxcl1					910:914	Cxcl1	910:914	Cxcl1	910:914	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	6	37	theme	microbial	1155:1163	arg1	composition					1165:1175	microbial composition	1155:1175	microbial composition	1155:1175	Moreover, no significant differences in microbial composition were observed between the genotypes.
35055106	0	38	theme	Mucosal	16:22	arg1	Injury					24:29	Colonic Mucosal Injury	8:29	Colonic Mucosal Injury	8:29	Reduced Colonic Mucosal Injury in 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice.
35055106	5	39	theme	target	1073:1078	arg1	gene					1080:1083	the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1065:1112	the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1065:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	4	40	theme	weight	755:760	arg1	loss					762:765	less body weight loss	745:765	less body weight loss	745:765	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	3	41	theme	-induced	615:622	arg1	model					632:636	a murine dextran sulfate sodium (DSS)-induced colitis model	578:636	a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation	578:715	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	1	42	theme	Poly-ADP-ribose	128:142	arg1	PARPs					157:161	PARPs	157:161	PARPs	157:161	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	42	theme	Poly-ADP-ribose	128:142	arg1	polymerases					144:154	Poly-ADP-ribose polymerases	128:154	Poly-ADP-ribose polymerases (PARPs)	128:162	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	42	theme	Poly-ADP-ribose	128:142	arg1	regulators					178:187	important regulators	168:187	important regulators	168:187	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	42	theme	Poly-ADP-ribose	128:142	arg1	polymerase					252:261	TCDD-inducible poly-ADP-ribose polymerase	221:261	TCDD-inducible poly-ADP-ribose polymerase (TIPARP)	221:270	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	43	theme	TCDD-inducible	221:234	arg1	TIPARP					264:269	TIPARP	264:269	TIPARP	264:269	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	43	theme	TCDD-inducible	221:234	arg1	polymerase					252:261	TCDD-inducible poly-ADP-ribose polymerase	221:261	TCDD-inducible poly-ADP-ribose polymerase (TIPARP)	221:270	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	0	44	theme	2,3,7,8-Tetrachlorodibenzo-p-Dioxin	34:68	arg1	Polymerase					86:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	Reduced Colonic Mucosal Injury in 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice.
35055106	3	45	theme	colitis	624:630	arg1	model					632:636	a murine dextran sulfate sodium (DSS)-induced colitis model	578:636	a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation	578:715	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	1	46	theme	poly-ADP-ribose	236:250	arg1	TIPARP					264:269	TIPARP	264:269	TIPARP	264:269	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	46	theme	poly-ADP-ribose	236:250	arg1	polymerase					252:261	TCDD-inducible poly-ADP-ribose polymerase	221:261	TCDD-inducible poly-ADP-ribose polymerase (TIPARP)	221:270	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	5	47	theme	P450	1096:1099	arg1	Cyp1a1					1106:1111	Cyp1a1	1106:1111	Cyp1a1	1106:1111	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	5	47	theme	P450	1096:1099	arg1	1A1					1101:1103	cytochrome P450 1A1	1085:1103	the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1065:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	3	48	theme	murine	580:585	arg1	model					632:636	a murine dextran sulfate sodium (DSS)-induced colitis model	578:636	a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation	578:715	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	5	49	from	genotypes	1026:1034	arg1	expression					1051:1060	the colonic expression	1039:1060	the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1)	1039:1112	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	0	50	theme	ADP-Ribose	75:84	arg1	Polymerase					86:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	Reduced Colonic Mucosal Injury in 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice.
35055106	7	51	theme	impaired	1287:1294	arg1	response					1303:1310	an impaired immune response	1284:1310	an impaired immune response to colonic inflammation	1284:1334	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	7	52	theme	colonic	1315:1321	arg1	inflammation					1323:1334	colonic inflammation	1315:1334	colonic inflammation	1315:1334	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	3	53	used	used	573:576	arg2	we					570:571	we	570:571	we	570:571	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	0	54	theme	Poly	70:73	arg1	Polymerase					86:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase	34:95	Reduced Colonic Mucosal Injury in 2,3,7,8-Tetrachlorodibenzo-p-Dioxin Poly ADP-Ribose Polymerase (TIPARP/PARP7)-Deficient Mice.
35055106	4	55	contain	had	741:743	arg2	loss					762:765	less body weight loss	745:765	less body weight loss	745:765	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	55	contain	had	741:743	arg2	scores					788:793	lower disease index scores	768:793	lower disease index scores	768:793	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	55	contain	had	741:743	arg1	mice					736:739	DSS-exposed Parp7 mice	718:739	DSS-exposed Parp7 mice	718:739	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	55	contain	had	741:743	arg2	expression					808:817	reduced expression	800:817	reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2,	800:933	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	56	theme	interleukin	860:870	arg1	IL-6					872:875	interleukin IL-6	860:875	interleukin IL-6	860:875	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	5	57	theme	significant	982:992	arg1	difference					994:1003	no significant difference	979:1003	no significant difference	979:1003	However, no significant difference was observed between genotypes in the colonic expression of the AHR target gene cytochrome P450 1A1 (Cyp1a1).
35055106	4	58	theme	reduced	800:806	arg1	expression					808:817	reduced expression	800:817	reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2,	800:933	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	2	59	theme	hydrocarbon	357:367	arg1	AHR					379:381	AHR	379:381	AHR	379:381	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	2	59	theme	hydrocarbon	357:367	arg1	receptor					369:376	aryl hydrocarbon receptor	352:376	aryl hydrocarbon receptor (AHR)	352:382	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	7	60	theme	IFN-I	1469:1473	arg1	signaling					1475:1483	IFN-I signaling	1469:1483	IFN-I signaling rather than AHR signaling	1469:1509	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	7	61	theme	immune	1296:1301	arg1	response					1303:1310	an impaired immune response	1284:1310	an impaired immune response to colonic inflammation	1284:1334	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	4	62	theme	C-x-c	878:882	arg1	ligand					900:905	C-x-c motif chemokine ligand 1	878:907	C-x-c motif chemokine ligand 1 (Cxcl1)	878:915	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	62	theme	C-x-c	878:882	arg1	Cxcl1					910:914	Cxcl1	910:914	Cxcl1	910:914	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	4	63	theme	index	782:786	arg1	scores					788:793	lower disease index scores	768:793	lower disease index scores	768:793	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	2	64	theme	aryl	352:355	arg1	AHR					379:381	AHR	379:381	AHR	379:381	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	2	64	theme	aryl	352:355	arg1	receptor					369:376	aryl hydrocarbon receptor	352:376	aryl hydrocarbon receptor (AHR)	352:382	PARP7 negatively regulates aryl hydrocarbon receptor (AHR) and type I interferon (IFN-I) signaling, both of which have been implicated in intestinal homeostasis and immunity.
35055106	3	65	theme	expression	524:533	arg1	loss					510:513	the loss	506:513	the loss of PARP7 expression	506:533	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	4	66	theme	Parp7	730:734	arg1	mice					736:739	DSS-exposed Parp7 mice	718:739	DSS-exposed Parp7 mice	718:739	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	6	67	from	differences	1140:1150	arg1	composition					1165:1175	microbial composition	1155:1175	microbial composition	1155:1175	Moreover, no significant differences in microbial composition were observed between the genotypes.
35055106	4	68	theme	disease	774:780	arg1	scores					788:793	lower disease index scores	768:793	lower disease index scores	768:793	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	1	69	theme	immune	196:201	arg1	system					203:208	the immune system	192:208	the immune system	192:208	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	3	70	theme	PARP7	667:671	arg1	loss					673:676	PARP7 loss	667:676	PARP7 loss	667:676	Since the loss of PARP7 expression increases AHR and IFN-I signaling, we used a murine dextran sulfate sodium (DSS)-induced colitis model to investigate the effect of PARP7 loss on DSS-induced intestinal inflammation.
35055106	4	71	theme	lower	768:772	arg1	scores					788:793	lower disease index scores	768:793	lower disease index scores	768:793	DSS-exposed Parp7 mice had less body weight loss, lower disease index scores, and reduced expression of several inflammation genes, including interleukin IL-6, C-x-c motif chemokine ligand 1 (Cxcl1), and lipocalin-2, when compared with wild-type mice.
35055106	1	72	theme	important	168:176	arg1	polymerases					144:154	Poly-ADP-ribose polymerases	128:154	Poly-ADP-ribose polymerases (PARPs)	128:162	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	72	theme	important	168:176	arg1	regulators					178:187	important regulators	168:187	important regulators	168:187	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	1	72	theme	important	168:176	arg1	polymerase					252:261	TCDD-inducible poly-ADP-ribose polymerase	221:261	TCDD-inducible poly-ADP-ribose polymerase (TIPARP)	221:270	Poly-ADP-ribose polymerases (PARPs) are important regulators of the immune system, including TCDD-inducible poly-ADP-ribose polymerase (TIPARP), also known as poly-ADP-ribose polymerase 7 (PARP7).
35055106	7	73	from	role	1461:1464	arg1	signaling					1475:1483	IFN-I signaling	1469:1483	IFN-I signaling rather than AHR signaling	1469:1509	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35055106	7	73	from	role	1461:1464	arg1	signaling					1501:1509	AHR signaling	1497:1509	IFN-I signaling rather than AHR signaling	1469:1509	Our findings demonstrate that the absence of PARP7 protein results in an impaired immune response to colonic inflammation and suggests that PARP7 may participate in the recruitment of immune cells to the inflammation site, which may be due to its role in IFN-I signaling rather than AHR signaling.
35327314	0	0	theme	Near-Infrared	78:90	arg1	Spectroscopy					92:103	Near-Infrared Spectroscopy	78:103	Near-Infrared Spectroscopy	78:103	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	3	1	theme	Traditional	303:313	arg1	methods					315:321	Traditional methods	303:321	Traditional methods	303:321	Traditional methods are not able to perform quality evaluation for this fungus in a short time, and a new method is needed for rapid quality assessment.
35327314	7	2	from	%	1267:1267	arg1	ASE					1231:1233	ASE	1231:1233	ASE	1231:1233	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	2	from	%	1267:1267	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	2	from	%	1267:1267	arg1	PSC					1221:1223	PSC	1221:1223	PSC	1221:1223	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	2	from	%	1267:1267	arg1	SFT					1239:1241	SFT	1239:1241	SFT	1239:1241	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	2	from	%	1267:1267	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	2	from	%	1267:1267	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	2	from	%	1267:1267	arg1	WSE					1226:1228	WSE	1226:1228	WSE	1226:1228	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	1	3	theme	important	146:154	arg1	fungus					156:161	an important fungus	143:161	an important fungus with high medicinal and nutritional values	143:204	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	1	3	theme	important	146:154	arg1	cocos					129:133	Poria cocos	123:133	Poria cocos (PC)	123:138	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	8	4	theme	PC	1456:1457	arg1	quality					1459:1465	PC quality	1456:1465	PC quality	1456:1465	Overall, a new approach was developed and validated which is able to distinguish PC production regions, quantify its chemical contents, and effectively evaluate PC quality.
35327314	4	5	theme	chemometric	517:527	arg1	method					529:534	chemometric method	517:534	chemometric method	517:534	Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions.
35327314	7	6	theme	WSE	1226:1228	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	6	theme	WSE	1226:1228	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	6	theme	WSE	1226:1228	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	5	7	theme	cultivation	674:684	arg1	regions					686:692	their cultivation regions	668:692	their cultivation regions	668:692	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	7	8	theme	models	1078:1083	arg1	performance					1057:1067	The performance	1053:1067	The performance of these models	1053:1083	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	9	from	coefficients	1116:1127	arg1	%					1251:1251	4.055%	1246:1251	4.055%	1246:1251	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	9	from	coefficients	1116:1127	arg1	%					1259:1259	3.821%	1254:1259	3.821%	1254:1259	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	9	from	coefficients	1116:1127	arg1	%					1267:1267	4.344%	1262:1267	4.344%	1262:1267	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	9	from	coefficients	1116:1127	arg1	%					1278:1278	3.744%	1273:1278	3.744%	1273:1278	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	2	10	theme	multiple	258:265	arg1	factors					267:273	multiple factors	258:273	multiple factors in the cultivation regions	258:300	However, the quality of PC is heavily dependent on multiple factors in the cultivation regions.
35327314	6	11	theme	quantitative	883:894	arg1	models					896:901	quantitative models	883:901	quantitative models	883:901	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	12	used	used	830:833	arg2	we					827:828	we	827:828	we	827:828	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	3	13	theme	quality	436:442	arg1	assessment					444:453	rapid quality assessment	430:453	rapid quality assessment	430:453	Traditional methods are not able to perform quality evaluation for this fungus in a short time, and a new method is needed for rapid quality assessment.
35327314	6	14	theme	ethanol-soluble	959:973	arg1	ASE					984:986	ASE	984:986	ASE	984:986	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	14	theme	ethanol-soluble	959:973	arg1	extract					975:981	ethanol-soluble extract	959:981	ethanol-soluble extract (ASE)	959:987	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	5	15	theme	high	794:797	arg1	accuracy					782:789	an accuracy	779:789	an accuracy as high as 92.59%	779:807	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	5	16	theme	forest	754:759	arg1	RFM					769:771	RFM	769:771	RFM	769:771	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	5	16	theme	forest	754:759	arg1	method					761:766	random forest method	747:766	random forest method (RFM)	747:772	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	6	17	theme	triterpenoids	1032:1044	arg1	PSC					1007:1009	PSC	1007:1009	PSC	1007:1009	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	17	theme	triterpenoids	1032:1044	arg1	extract					944:950	water-soluble extract	930:950	water-soluble extract (WSE)	930:956	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	17	theme	triterpenoids	1032:1044	arg1	WSE					953:955	WSE	953:955	WSE	953:955	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	17	theme	triterpenoids	1032:1044	arg1	ASE					984:986	ASE	984:986	ASE	984:986	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	17	theme	triterpenoids	1032:1044	arg1	SFT					1047:1049	SFT	1047:1049	SFT	1047:1049	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	17	theme	triterpenoids	1032:1044	arg1	polysaccharides					990:1004	polysaccharides	990:1004	polysaccharides (PSC)	990:1010	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	17	theme	triterpenoids	1032:1044	arg1	sum					1020:1022	the sum	1016:1022	the sum of five triterpenoids (SFT)	1016:1050	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	17	theme	triterpenoids	1032:1044	arg1	extract					975:981	ethanol-soluble extract	959:981	ethanol-soluble extract (ASE)	959:987	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	18	theme	square	849:854	arg1	regression					856:865	partial least square regression	835:865	partial least square regression (PLSR)	835:872	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	18	theme	square	849:854	arg1	PLSR					868:871	PLSR	868:871	PLSR	868:871	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	7	19	from	%	1251:1251	arg1	ASE					1231:1233	ASE	1231:1233	ASE	1231:1233	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	19	from	%	1251:1251	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	19	from	%	1251:1251	arg1	PSC					1221:1223	PSC	1221:1223	PSC	1221:1223	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	19	from	%	1251:1251	arg1	SFT					1239:1241	SFT	1239:1241	SFT	1239:1241	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	19	from	%	1251:1251	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	19	from	%	1251:1251	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	19	from	%	1251:1251	arg1	WSE					1226:1228	WSE	1226:1228	WSE	1226:1228	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	5	20	theme	NIR	726:728	arg1	spectroscopy					730:741	NIR spectroscopy	726:741	NIR spectroscopy	726:741	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	4	21	used	used	465:468	arg2	we					462:463	we	462:463	we	462:463	Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions.
35327314	1	22	theme	high	168:171	arg1	values					199:204	high medicinal and nutritional values	168:204	high medicinal and nutritional values	168:204	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	7	23	from	%	1259:1259	arg1	ASE					1231:1233	ASE	1231:1233	ASE	1231:1233	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	23	from	%	1259:1259	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	23	from	%	1259:1259	arg1	PSC					1221:1223	PSC	1221:1223	PSC	1221:1223	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	23	from	%	1259:1259	arg1	SFT					1239:1241	SFT	1239:1241	SFT	1239:1241	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	23	from	%	1259:1259	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	23	from	%	1259:1259	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	23	from	%	1259:1259	arg1	WSE					1226:1228	WSE	1226:1228	WSE	1226:1228	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	2	24	theme	cultivation	282:292	arg1	regions					294:300	the cultivation regions	278:300	the cultivation regions	278:300	However, the quality of PC is heavily dependent on multiple factors in the cultivation regions.
35327314	1	25	theme	medicinal	173:181	arg1	values					199:204	high medicinal and nutritional values	168:204	high medicinal and nutritional values	168:204	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	3	26	theme	quality	347:353	arg1	evaluation					355:364	quality evaluation	347:364	quality evaluation	347:364	Traditional methods are not able to perform quality evaluation for this fungus in a short time, and a new method is needed for rapid quality assessment.
35327314	7	27	theme	ASE	1231:1233	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	27	theme	ASE	1231:1233	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	27	theme	ASE	1231:1233	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	5	28	theme	samples	641:647	arg1	batches					630:636	138 batches	626:636	138 batches of samples	626:647	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	6	29	theme	sum	1020:1022	arg1	content					919:925	the content	915:925	the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT)	915:1050	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	4	30	theme	PC	586:587	arg1	compositions					598:609	PC chemical compositions	586:609	PC chemical compositions	586:609	Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions.
35327314	0	31	theme	Cultivation	17:27	arg1	Regions					29:35	Cultivation Regions	17:35	Cultivation Regions	17:35	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	7	32	theme	correlation	1104:1114	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	32	theme	correlation	1104:1114	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	32	theme	correlation	1104:1114	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	1	33	theme	nutritional	187:197	arg1	values					199:204	high medicinal and nutritional values	168:204	high medicinal and nutritional values	168:204	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	0	34	theme	Quality	41:47	arg1	Parameters					49:58	Quality Parameters	41:58	Quality Parameters	41:58	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	7	35	theme	quality	1170:1176	arg1	parameters					1178:1187	the four quality parameters	1161:1187	the four quality parameters	1161:1187	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	3	36	theme	new	405:407	arg1	method					409:414	a new method	403:414	a new method	403:414	Traditional methods are not able to perform quality evaluation for this fungus in a short time, and a new method is needed for rapid quality assessment.
35327314	3	37	theme	short	387:391	arg1	time					393:396	a short time	385:396	a short time	385:396	Traditional methods are not able to perform quality evaluation for this fungus in a short time, and a new method is needed for rapid quality assessment.
35327314	4	38	theme	near-infrared	470:482	arg1	spectroscopy					490:501	near-infrared (NIR) spectroscopy	470:501	near-infrared (NIR) spectroscopy combined with chemometric method	470:534	Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions.
35327314	8	39	theme	production	1379:1388	arg1	regions					1390:1396	PC production regions	1376:1396	PC production regions	1376:1396	Overall, a new approach was developed and validated which is able to distinguish PC production regions, quantify its chemical contents, and effectively evaluate PC quality.
35327314	0	40	theme	Regions	29:35	arg1	Determination					0:12	Determination	0:12	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.	0:121	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	4	41	theme	chemical	589:596	arg1	compositions					598:609	PC chemical compositions	586:609	PC chemical compositions	586:609	Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions.
35327314	5	42	theme	random	747:752	arg1	RFM					769:771	RFM	769:771	RFM	769:771	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	5	42	theme	random	747:752	arg1	method					761:766	random forest method	747:766	random forest method (RFM)	747:772	In our study, 138 batches of samples were collected and their cultivation regions were distinguished by combining NIR spectroscopy and random forest method (RFM) with an accuracy as high as 92.59%.
35327314	7	43	from	%	1278:1278	arg1	ASE					1231:1233	ASE	1231:1233	ASE	1231:1233	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	43	from	%	1278:1278	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	43	from	%	1278:1278	arg1	PSC					1221:1223	PSC	1221:1223	PSC	1221:1223	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	43	from	%	1278:1278	arg1	SFT					1239:1241	SFT	1239:1241	SFT	1239:1241	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	43	from	%	1278:1278	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	43	from	%	1278:1278	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	43	from	%	1278:1278	arg1	WSE					1226:1228	WSE	1226:1228	WSE	1226:1228	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	1	44	theme	Poria	123:127	arg1	fungus					156:161	an important fungus	143:161	an important fungus with high medicinal and nutritional values	143:204	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	1	44	theme	Poria	123:127	arg1	PC					136:137	PC	136:137	PC	136:137	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	1	44	theme	Poria	123:127	arg1	cocos					129:133	Poria cocos	123:133	Poria cocos (PC)	123:138	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	0	45	theme	Parameters	49:58	arg1	Determination					0:12	Determination	0:12	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.	0:121	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	4	46	theme	NIR	485:487	arg1	spectroscopy					490:501	near-infrared (NIR) spectroscopy	470:501	near-infrared (NIR) spectroscopy combined with chemometric method	470:534	Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions.
35327314	8	47	theme	chemical	1412:1419	arg1	contents					1421:1428	its chemical contents	1408:1428	its chemical contents	1408:1428	Overall, a new approach was developed and validated which is able to distinguish PC production regions, quantify its chemical contents, and effectively evaluate PC quality.
35327314	6	48	theme	least	843:847	arg1	regression					856:865	partial least square regression	835:865	partial least square regression (PLSR)	835:872	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	48	theme	least	843:847	arg1	PLSR					868:871	PLSR	868:871	PLSR	868:871	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	1	49	with	fungus	156:161	arg1	values					199:204	high medicinal and nutritional values	168:204	high medicinal and nutritional values	168:204	Poria cocos (PC) is an important fungus with high medicinal and nutritional values.
35327314	7	50	theme	PSC	1221:1223	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	50	theme	PSC	1221:1223	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	50	theme	PSC	1221:1223	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	4	51	theme	cultivation	552:562	arg1	regions					564:570	the cultivation regions	548:570	the cultivation regions	548:570	Here, we used near-infrared (NIR) spectroscopy combined with chemometric method to identify the cultivation regions and determine PC chemical compositions.
35327314	7	52	theme	SFT	1239:1241	arg1	coefficients					1116:1127	correlation coefficients	1104:1127	correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively	1104:1292	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	52	theme	SFT	1239:1241	arg1	R2pre					1140:1144	R2pre	1140:1144	R2pre	1140:1144	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	7	52	theme	SFT	1239:1241	arg1	R2cal					1130:1134	R2cal	1130:1134	R2cal	1130:1134	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	3	53	theme	rapid	430:434	arg1	assessment					444:453	rapid quality assessment	430:453	rapid quality assessment	430:453	Traditional methods are not able to perform quality evaluation for this fungus in a short time, and a new method is needed for rapid quality assessment.
35327314	2	54	from	factors	267:273	arg1	regions					294:300	the cultivation regions	278:300	the cultivation regions	278:300	However, the quality of PC is heavily dependent on multiple factors in the cultivation regions.
35327314	0	55	theme	cocos	69:73	arg1	Regions					29:35	Cultivation Regions	17:35	Cultivation Regions	17:35	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	0	55	theme	cocos	69:73	arg1	Parameters					49:58	Quality Parameters	41:58	Quality Parameters	41:58	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	6	56	theme	extract	944:950	arg1	content					919:925	the content	915:925	the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT)	915:1050	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	2	57	theme	PC	231:232	arg1	dependent					245:253	dependent	245:253	dependent	245:253	However, the quality of PC is heavily dependent on multiple factors in the cultivation regions.
35327314	2	57	theme	PC	231:232	arg1	quality					220:226	the quality	216:226	the quality of PC	216:232	However, the quality of PC is heavily dependent on multiple factors in the cultivation regions.
35327314	8	58	theme	new	1306:1308	arg1	approach					1310:1317	a new approach	1304:1317	a new approach	1304:1317	Overall, a new approach was developed and validated which is able to distinguish PC production regions, quantify its chemical contents, and effectively evaluate PC quality.
35327314	6	59	theme	polysaccharides	990:1004	arg1	content					919:925	the content	915:925	the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT)	915:1050	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	60	theme	extract	975:981	arg1	content					919:925	the content	915:925	the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT)	915:1050	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	8	61	theme	PC	1376:1377	arg1	regions					1390:1396	PC production regions	1376:1396	PC production regions	1376:1396	Overall, a new approach was developed and validated which is able to distinguish PC production regions, quantify its chemical contents, and effectively evaluate PC quality.
35327314	0	62	theme	Poria	63:67	arg1	cocos					69:73	Poria cocos	63:73	Poria cocos	63:73	Determination of Cultivation Regions and Quality Parameters of Poria cocos by Near-Infrared Spectroscopy and Chemometrics.
35327314	6	63	theme	partial	835:841	arg1	regression					856:865	partial least square regression	835:865	partial least square regression (PLSR)	835:872	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	63	theme	partial	835:841	arg1	PLSR					868:871	PLSR	868:871	PLSR	868:871	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	7	64	theme	relative	1197:1204	arg1	errors					1206:1211	the relative errors	1193:1211	the relative errors	1193:1211	The performance of these models were verified with correlation coefficients (R2cal and R2pre) above 0.9 for the four quality parameters and the relative errors (RE) of PSC, WSE, ASE and SFT at 4.055%, 3.821%, 4.344% and 3.744%, respectively.
35327314	6	65	theme	water-soluble	930:942	arg1	WSE					953:955	WSE	953:955	WSE	953:955	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35327314	6	65	theme	water-soluble	930:942	arg1	extract					944:950	water-soluble extract	930:950	water-soluble extract (WSE)	930:956	In the meantime, we used partial least square regression (PLSR) to build quantitative models and measure the content of water-soluble extract (WSE), ethanol-soluble extract (ASE), polysaccharides (PSC) and the sum of five triterpenoids (SFT).
35661288	7	0	from	changes	1536:1542	arg1	role					1595:1598	cooperative or antagonist role	1569:1598	cooperative or antagonist role	1569:1598	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	0	from	changes	1536:1542	arg1	expression					1551:1560	expression	1551:1560	expression	1551:1560	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	0	from	changes	1536:1542	arg1	Osteopontin					1625:1635	Osteopontin	1625:1635	Osteopontin	1625:1635	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	0	from	changes	1536:1542	arg1	Osteocalcin					1612:1622	Osteocalcin	1612:1622	Osteocalcin	1612:1622	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	0	from	changes	1536:1542	arg1	Sialoprotein-2					1647:1660	Bone Sialoprotein-2	1642:1660	Bone Sialoprotein-2	1642:1660	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	3	1	theme	further	500:506	arg1	insight					508:514	further insight	500:514	further insight into their expression	500:536	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	8	2	theme	FTIRI	1878:1882	arg1	capability					1898:1907	the FTIRI translational capability	1874:1907	the FTIRI translational capability	1874:1907	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	8	3	theme	translational	1884:1896	arg1	capability					1898:1907	the FTIRI translational capability	1874:1907	the FTIRI translational capability	1874:1907	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	6	4	theme	innovatory	1261:1270	arg1	procedure					1272:1280	This innovatory procedure	1256:1280	This innovatory procedure	1256:1280	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	5	5	from	matrix	1031:1036	arg1	conditions					1056:1065	healthy and OP conditions	1041:1065	healthy and OP conditions	1041:1065	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	1	6	theme	organic	204:210	arg1	components					226:235	organic and inorganic components	204:235	organic and inorganic components of the extracellular matrix (ECM)	204:269	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	7	7	theme	deep	1675:1678	arg1	impact					1680:1685	the deep impact	1671:1685	the deep impact of these NCPs on collagen features of OP bone	1671:1731	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	8	8	theme	traditional	1933:1943	arg1	methods					1956:1962	traditional diagnostic methods	1933:1962	traditional diagnostic methods	1933:1962	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	2	9	theme	balance	301:307	arg1	Alteration					282:291	Alteration	282:291	Alteration of this balance	282:307	Alteration of this balance has consequences on bone mass and architecture, resulting in conditions such as osteoporosis (OP).
35661288	1	10	theme	inorganic	216:224	arg1	components					226:235	organic and inorganic components	204:235	organic and inorganic components of the extracellular matrix (ECM)	204:269	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	4	11	theme	Growth	939:944	arg1	Decorin					854:860	Decorin	854:860	Decorin	854:860	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	4	11	theme	Growth	939:944	arg1	beta					953:956	Transforming Growth Factor beta	926:956	Transforming Growth Factor beta	926:956	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	7	12	from	impact	1680:1685	arg1	features					1713:1720	collagen features	1704:1720	collagen features of OP bone	1704:1731	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	4	13	theme	I	648:648	arg1	Collagen					650:657	Type I Collagen	643:657	Type I Collagen	643:657	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	0	14	theme	correlated	139:148	arg1	analyses					150:157	histomorphometric and Fourier transform infrared imaging correlated analyses	82:157	histomorphometric and Fourier transform infrared imaging correlated analyses	82:157	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	3	15	theme	pathological	596:607	arg1	conditions					609:618	normal and pathological conditions	585:618	normal and pathological conditions	585:618	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	7	16	theme	Bone	1642:1645	arg1	expression					1551:1560	expression	1551:1560	expression	1551:1560	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	16	theme	Bone	1642:1645	arg1	Sialoprotein-2					1647:1660	Bone Sialoprotein-2	1642:1660	Bone Sialoprotein-2	1642:1660	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	5	17	theme	healthy	1041:1047	arg1	conditions					1056:1065	healthy and OP conditions	1041:1065	healthy and OP conditions	1041:1065	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	3	18	theme	normal	585:590	arg1	conditions					609:618	normal and pathological conditions	585:618	normal and pathological conditions	585:618	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	6	19	theme	tissue	1323:1328	arg1	sections					1330:1337	superimposed tissue sections	1310:1337	superimposed tissue sections	1310:1337	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	5	20	theme	OP	1053:1054	arg1	conditions					1056:1065	healthy and OP conditions	1041:1065	healthy and OP conditions	1041:1065	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	0	21	theme	femoral	57:63	arg1	head					65:68	osteoporotic human femoral head	38:68	osteoporotic human femoral head	38:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	5	22	theme	Imaging	1133:1139	arg1	technique					1149:1157	Imaging (FTIRI) technique	1133:1157	Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting	1133:1253	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	8	23	theme	clinical	1810:1817	arg1	strategies					1819:1828	targeted clinical strategies	1801:1828	targeted clinical strategies aimed at bone mass preservation	1801:1860	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	1	24	theme	Bone	160:163	arg1	homeostasis					165:175	Bone homeostasis	160:175	Bone homeostasis	160:175	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	1	24	theme	Bone	160:163	arg1	equilibrium					184:194	the equilibrium	180:194	the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells	180:279	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	4	25	theme	structural	692:701	arg1	properties					703:712	structural properties	692:712	structural properties	692:712	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	5	26	theme	FTIRI	1142:1146	arg1	technique					1149:1157	Imaging (FTIRI) technique	1133:1157	Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting	1133:1253	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	7	27	theme	OP	1725:1726	arg1	bone					1728:1731	OP bone	1725:1731	OP bone	1725:1731	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	6	28	theme	tissue	1384:1389	arg1	architecture					1391:1402	OP bone tissue architecture	1376:1402	OP bone tissue architecture	1376:1402	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	8	29	theme	bone	1839:1842	arg1	preservation					1849:1860	bone mass preservation	1839:1860	bone mass preservation	1839:1860	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	0	30	dep	Fourier	104:110	arg1	transform					112:120	transform	112:120	transform infrared imaging	112:137	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	7	31	theme	antagonist	1584:1593	arg1	role					1595:1598	cooperative or antagonist role	1569:1598	cooperative or antagonist role	1569:1598	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	4	32	theme	matrix	778:783	arg1	composition					785:795	matrix composition	778:795	matrix composition	778:795	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	3	33	theme	mutual	426:431	arg1	regulation					433:442	ECM protein mutual regulation	414:442	ECM protein mutual regulation	414:442	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	6	34	theme	OP	1376:1377	arg1	architecture					1391:1402	OP bone tissue architecture	1376:1402	OP bone tissue architecture	1376:1402	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	5	35	theme	multidisciplinary	980:996	arg1	approach					998:1005	a novel multidisciplinary approach	972:1005	a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting	972:1253	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	0	36	from	networking	24:33	arg1	head					65:68	osteoporotic human femoral head	38:68	osteoporotic human femoral head	38:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	3	37	theme	ECM	414:416	arg1	regulation					433:442	ECM protein mutual regulation	414:442	ECM protein mutual regulation	414:442	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	0	38	theme	Altered	0:6	arg1	networking					24:33	Altered type I collagen networking	0:33	Altered type I collagen networking in osteoporotic human femoral head	0:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	0	39	theme	I	13:13	arg1	collagen					15:22	type I collagen	8:22	Altered type I collagen networking in osteoporotic human femoral head	0:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	5	40	theme	Red	1198:1200	arg1	staining					1202:1209	Sirius Red staining	1191:1209	Sirius Red staining	1191:1209	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	6	41	dep	Collagen	1439:1446	arg1	structure					1448:1456	structure	1448:1456	structure	1448:1456	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	5	42	theme	bone	1026:1029	arg1	matrix					1031:1036	bone matrix	1026:1036	bone matrix in healthy and OP conditions	1026:1065	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	6	43	theme	Type	1432:1435	arg1	Collagen					1439:1446	warped Type I Collagen structure and deposition	1425:1471	Collagen	1439:1446	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	5	44	dep	Fourier	1106:1112	arg1	Transform					1114:1122	Transform	1114:1122	Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting	1114:1253	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	5	45	theme	Western	1238:1244	arg1	Blotting					1246:1253	Western Blotting	1238:1253	Western Blotting	1238:1253	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	0	46	dep	correlated	139:148	arg1	Fourier					104:110	Fourier	104:110	Fourier transform infrared imaging	104:137	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	6	47	theme	total	1501:1505	arg1	amount					1515:1520	the total protein amount	1497:1520	the total protein amount	1497:1520	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	1	48	theme	matrix	258:263	arg1	components					226:235	organic and inorganic components	204:235	organic and inorganic components of the extracellular matrix (ECM)	204:269	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	1	48	theme	matrix	258:263	arg1	cells					275:279	cells	275:279	cells	275:279	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	6	49	theme	I	1437:1437	arg1	Collagen					1439:1446	warped Type I Collagen structure and deposition	1425:1471	Collagen	1439:1446	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	6	50	theme	superimposed	1310:1321	arg1	sections					1330:1337	superimposed tissue sections	1310:1337	superimposed tissue sections	1310:1337	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	3	51	from	regulation	433:442	arg1	bone					465:468	bone structure and mineralization	465:497	bone	465:468	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	3	51	from	regulation	433:442	arg1	mineralization					484:497	mineralization	484:497	mineralization	484:497	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	8	52	theme	upcoming	1912:1919	arg1	support					1921:1927	upcoming support	1912:1927	upcoming support for traditional diagnostic methods	1912:1962	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	0	53	theme	osteoporotic	38:49	arg1	head					65:68	osteoporotic human femoral head	38:68	osteoporotic human femoral head	38:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	3	54	theme	bone	566:569	arg1	biology					571:577	bone biology	566:577	bone biology	566:577	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	8	55	theme	diagnostic	1945:1954	arg1	methods					1956:1962	traditional diagnostic methods	1933:1962	traditional diagnostic methods	1933:1962	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	4	56	theme	Transforming	926:937	arg1	Decorin					854:860	Decorin	854:860	Decorin	854:860	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	4	56	theme	Transforming	926:937	arg1	beta					953:956	Transforming Growth Factor beta	926:956	Transforming Growth Factor beta	926:956	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	4	57	theme	Type	643:646	arg1	Collagen					650:657	Type I Collagen	643:657	Type I Collagen	643:657	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	4	58	theme	Bone	889:892	arg1	Decorin					854:860	Decorin	854:860	Decorin	854:860	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	4	58	theme	Bone	889:892	arg1	Sialoprotein					894:905	Bone Sialoprotein 2	889:907	Bone Sialoprotein 2	889:907	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	6	59	theme	sections	1330:1337	arg1	analysis					1298:1305	the analysis	1294:1305	the analysis of superimposed tissue sections	1294:1337	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	8	60	theme	starting	1772:1779	arg1	point					1781:1785	a starting point	1770:1785	a starting point for designing targeted clinical strategies aimed at bone mass preservation	1770:1860	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	3	61	dep	bone	465:468	arg1	structure					470:478	structure	470:478	structure	470:478	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	6	62	theme	warped	1425:1430	arg1	Collagen					1439:1446	warped Type I Collagen structure and deposition	1425:1471	Collagen	1439:1446	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	7	63	theme	Decorin	1603:1609	arg1	expression					1551:1560	expression	1551:1560	expression	1551:1560	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	63	theme	Decorin	1603:1609	arg1	Osteopontin					1625:1635	Osteopontin	1625:1635	Osteopontin	1625:1635	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	63	theme	Decorin	1603:1609	arg1	Osteocalcin					1612:1622	Osteocalcin	1612:1622	Osteocalcin	1612:1622	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	63	theme	Decorin	1603:1609	arg1	Sialoprotein-2					1647:1660	Bone Sialoprotein-2	1642:1660	Bone Sialoprotein-2	1642:1660	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	0	64	theme	histomorphometric	82:98	arg1	analyses					150:157	histomorphometric and Fourier transform infrared imaging correlated analyses	82:157	histomorphometric and Fourier transform infrared imaging correlated analyses	82:157	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	8	65	theme	targeted	1801:1808	arg1	strategies					1819:1828	targeted clinical strategies	1801:1828	targeted clinical strategies aimed at bone mass preservation	1801:1860	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	0	66	dep	transform	112:120	arg1	infrared					122:129	infrared	122:129	transform infrared imaging	112:137	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	7	67	theme	bone	1728:1731	arg1	features					1713:1720	collagen features	1704:1720	collagen features of OP bone	1704:1731	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	68	theme	or	1581:1582	arg1	role					1595:1598	cooperative or antagonist role	1569:1598	cooperative or antagonist role	1569:1598	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	4	69	theme	Factor	946:951	arg1	Decorin					854:860	Decorin	854:860	Decorin	854:860	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	4	69	theme	Factor	946:951	arg1	beta					953:956	Transforming Growth Factor beta	926:956	Transforming Growth Factor beta	926:956	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	6	70	theme	bone	1379:1382	arg1	architecture					1391:1402	OP bone tissue architecture	1376:1402	OP bone tissue architecture	1376:1402	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	5	71	theme	novel	974:978	arg1	approach					998:1005	a novel multidisciplinary approach	972:1005	a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting	972:1253	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	1	72	theme	extracellular	244:256	arg1	matrix					258:263	the extracellular matrix	240:263	the extracellular matrix (ECM)	240:269	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	1	72	theme	extracellular	244:256	arg1	ECM					266:268	ECM	266:268	ECM	266:268	Bone homeostasis is the equilibrium between organic and inorganic components of the extracellular matrix (ECM) and cells.
35661288	0	73	theme	type	8:11	arg1	collagen					15:22	type I collagen	8:22	Altered type I collagen networking in osteoporotic human femoral head	0:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	8	74	theme	mass	1844:1847	arg1	preservation					1849:1860	bone mass preservation	1839:1860	bone mass preservation	1839:1860	Overall, our strategy may represent a starting point for designing targeted clinical strategies aimed at bone mass preservation and sustain the FTIRI translational capability as upcoming support for traditional diagnostic methods.
35661288	7	75	theme	collagen	1704:1711	arg1	features					1713:1720	collagen features	1704:1720	collagen features of OP bone	1704:1731	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	4	76	theme	bone	736:739	arg1	mineralization					718:731	mineralization	718:731	mineralization of bone	718:739	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	4	76	theme	bone	736:739	arg1	properties					703:712	structural properties	692:712	structural properties	692:712	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	3	77	theme	protein	418:424	arg1	regulation					433:442	ECM protein mutual regulation	414:442	ECM protein mutual regulation	414:442	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	0	78	theme	collagen	15:22	arg1	networking					24:33	Altered type I collagen networking	0:33	Altered type I collagen networking in osteoporotic human femoral head	0:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	5	79	theme	Sirius	1191:1196	arg1	staining					1202:1209	Sirius Red staining	1191:1209	Sirius Red staining	1191:1209	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	7	80	theme	NCPs	1696:1699	arg1	impact					1680:1685	the deep impact	1671:1685	the deep impact of these NCPs on collagen features of OP bone	1671:1731	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	6	81	from	alterations	1361:1371	arg1	architecture					1391:1402	OP bone tissue architecture	1376:1402	OP bone tissue architecture	1376:1402	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	7	82	theme	cooperative	1569:1579	arg1	role					1595:1598	cooperative or antagonist role	1569:1598	cooperative or antagonist role	1569:1598	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	5	83	dep	Transform	1114:1122	arg1	Infrared					1124:1131	Infrared	1124:1131	Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting	1114:1253	We developed a novel multidisciplinary approach in order to assess bone matrix in healthy and OP conditions more comprehensively by exploiting the Fourier Transform Infrared Imaging (FTIRI) technique combined with histomorphometry, Sirius Red staining, immunohistochemistry, and Western Blotting.
35661288	0	84	theme	human	51:55	arg1	head					65:68	osteoporotic human femoral head	38:68	osteoporotic human femoral head	38:68	Altered type I collagen networking in osteoporotic human femoral head revealed by histomorphometric and Fourier transform infrared imaging correlated analyses.
35661288	2	85	contain	has	309:311	arg2	consequences					313:324	consequences	313:324	consequences	313:324	Alteration of this balance has consequences on bone mass and architecture, resulting in conditions such as osteoporosis (OP).
35661288	2	85	contain	has	309:311	arg1	Alteration					282:291	Alteration	282:291	Alteration of this balance	282:307	Alteration of this balance has consequences on bone mass and architecture, resulting in conditions such as osteoporosis (OP).
35661288	4	86	theme	cell-matrix	822:832	arg1	interaction					834:844	cell-matrix interaction	822:844	cell-matrix interaction	822:844	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
35661288	3	87	from	effects	454:460	arg1	bone					465:468	bone structure and mineralization	465:497	bone	465:468	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	3	87	from	effects	454:460	arg1	mineralization					484:497	mineralization	484:497	mineralization	484:497	Given ECM protein mutual regulation and their effects on bone structure and mineralization, further insight into their expression is crucial to understanding bone biology under normal and pathological conditions.
35661288	6	88	from	changes	1486:1492	arg1	amount					1515:1520	the total protein amount	1497:1520	the total protein amount	1497:1520	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	2	89	theme	bone	329:332	arg1	mass					334:337	bone mass	329:337	bone mass	329:337	Alteration of this balance has consequences on bone mass and architecture, resulting in conditions such as osteoporosis (OP).
35661288	6	90	theme	protein	1507:1513	arg1	amount					1515:1520	the total protein amount	1497:1520	the total protein amount	1497:1520	This innovatory procedure allowed for the analysis of superimposed tissue sections and revealed that the alterations in OP bone tissue architecture were associated with warped Type I Collagen structure and deposition but not with changes in the total protein amount.
35661288	7	91	dep	expression	1551:1560	arg1	the					1547:1549	the	1547:1549	the	1547:1549	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	7	92	theme	detected	1527:1534	arg1	changes					1536:1542	The detected changes	1523:1542	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2	1523:1660	The detected changes in the expression and/or cooperative or antagonist role of Decorin, Osteocalcin, Osteopontin, and Bone Sialoprotein-2 indicate the deep impact of these NCPs on collagen features of OP bone.
35661288	4	93	theme	mineral	798:804	arg1	deposition					806:815	mineral deposition	798:815	mineral deposition	798:815	This study focused on Type I Collagen, which is mainly responsible for structural properties and mineralization of bone, and selected proteins implicated in matrix composition, mineral deposition, and cell-matrix interaction such as Decorin, Osteocalcin, Osteopontin, Bone Sialoprotein 2, Osteonectin and Transforming Growth Factor beta.
36673515	10	0	theme	directed	1469:1476	arg1	enrichment					1478:1487	the directed enrichment	1465:1487	the directed enrichment of specific species in Bacteroides	1465:1522	Therefore, WMP might be suitable as a functional foods to prevent or delay aging via the directed enrichment of specific species in Bacteroides.
36673515	3	1	from	composition	405:415	arg1	mice					464:467	D-galactose-induced aging mice	438:467	D-galactose-induced aging mice	438:467	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	3	2	from	effects	376:382	arg1	composition					405:415	composition	405:415	composition	405:415	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	3	2	from	effects	376:382	arg1	diversity					391:399	diversity	391:399	diversity	391:399	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	4	3	theme	locomotor	497:505	arg1	activity					507:514	locomotor activity	497:514	locomotor activity	497:514	WMP significantly improved locomotor activity and the spatial and recognition memory of the aging mice.
36673515	4	4	theme	recognition	536:546	arg1	memory					548:553	the spatial and recognition memory	520:553	memory	548:553	WMP significantly improved locomotor activity and the spatial and recognition memory of the aging mice.
36673515	8	5	theme	PICRUSt	1012:1018	arg1	analysis					1020:1027	A PICRUSt analysis	1010:1027	A PICRUSt analysis	1010:1027	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	7	6	theme	species	876:882	arg1	level					884:888	the species level	872:888	the species level	872:888	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	0	7	theme	Gut	114:116	arg1	Microbiota					118:127	Gut Microbiota	114:127	Gut Microbiota	114:127	Agaricus bisporus Polysaccharides Ameliorates Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota.
36673515	7	8	theme	acidifaciens	940:951	arg1	enrichments					922:932	the enrichments	918:932	the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris	918:996	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	9	9	from	abundance	1287:1295	arg1	levels					1272:1277	SCFA levels	1267:1277	SCFA levels	1267:1277	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	7	10	theme	nbsp	958:961	arg1	enrichments					922:932	the enrichments	918:932	the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris	918:996	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	8	11	from	pathway	1163:1169	arg1	metabolism					1075:1084	the metabolism	1071:1084	the metabolism of carbon	1071:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	9	12	theme	Helicobacter	1366:1377	arg1	changes					1256:1262	the behavioral improvements and changes	1224:1262	changes	1256:1262	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	12	theme	Helicobacter	1366:1377	arg1	abundance					1287:1295	the abundance	1283:1295	the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter	1283:1377	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	12	theme	Helicobacter	1366:1377	arg1	improvements					1239:1250	the behavioral improvements and changes	1224:1262	improvements	1239:1250	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	8	13	theme	amino	1112:1116	arg1	impact					1061:1066	a greater impact	1051:1066	a greater impact on the metabolism of carbon	1051:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	13	theme	amino	1112:1116	arg1	acid					1118:1121	amino acid	1112:1121	amino acid	1112:1121	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	1	14	contain	has	173:175	arg1	WMP					168:170	WMP	168:170	WMP	168:170	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	1	14	contain	has	173:175	arg1	polysaccharide					152:165	White button mushroom polysaccharide	130:165	White button mushroom polysaccharide (WMP)	130:171	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	1	14	contain	has	173:175	arg2	functions					202:210	various health-promoting functions	177:210	various health-promoting functions	177:210	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	3	15	dep	diversity	391:399	arg1	the					387:389	the	387:389	the	387:389	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	5	16	theme	pro-inflammatory	628:643	arg1	levels					654:659	the pro-inflammatory cytokine levels	624:659	the pro-inflammatory cytokine levels in the brain	624:672	It also alleviated oxidative stress and decreased the pro-inflammatory cytokine levels in the brain.
36673515	7	17	from	level	884:888	arg1	effect					847:852	its effect	843:852	its effect on Bacteroides at the species level	843:888	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	10	18	theme	species	1501:1507	arg1	enrichment					1478:1487	the directed enrichment	1465:1487	the directed enrichment of specific species in Bacteroides	1465:1522	Therefore, WMP might be suitable as a functional foods to prevent or delay aging via the directed enrichment of specific species in Bacteroides.
36673515	5	19	theme	cytokine	645:652	arg1	levels					654:659	the pro-inflammatory cytokine levels	624:659	the pro-inflammatory cytokine levels in the brain	624:672	It also alleviated oxidative stress and decreased the pro-inflammatory cytokine levels in the brain.
36673515	6	20	theme	acid	734:737	arg1	level					746:750	the short-chain fatty acid (SCFA) level	712:750	the short-chain fatty acid (SCFA) level	712:750	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	6	20	theme	acid	734:737	arg1	α-diversity					699:709	α-diversity	699:709	α-diversity	699:709	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	1	21	theme	various	177:183	arg1	functions					202:210	various health-promoting functions	177:210	various health-promoting functions	177:210	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	3	22	theme	WMP	364:366	arg1	effects					376:382	its effects	372:382	its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice	372:467	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	3	22	theme	WMP	364:366	arg1	capacity					352:359	the anti-aging capacity	337:359	the anti-aging capacity of WMP	337:366	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	0	23	from	Deficits	58:65	arg1	Mice					96:99	D-Galactose-Induced Aging Mice	70:99	D-Galactose-Induced Aging Mice	70:99	Agaricus bisporus Polysaccharides Ameliorates Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota.
36673515	9	24	theme	Desulfovibrio	1348:1360	arg1	changes					1256:1262	the behavioral improvements and changes	1224:1262	changes	1256:1262	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	24	theme	Desulfovibrio	1348:1360	arg1	abundance					1287:1295	the abundance	1283:1295	the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter	1283:1377	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	24	theme	Desulfovibrio	1348:1360	arg1	improvements					1239:1250	the behavioral improvements and changes	1224:1262	improvements	1239:1250	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	10	25	theme	functional	1418:1427	arg1	foods					1429:1433	a functional foods	1416:1433	a functional foods to prevent or delay aging via the directed enrichment of specific species in Bacteroides	1416:1522	Therefore, WMP might be suitable as a functional foods to prevent or delay aging via the directed enrichment of specific species in Bacteroides.
36673515	8	26	from	impact	1061:1066	arg1	metabolism					1075:1084	the metabolism	1071:1084	the metabolism of carbon	1071:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	6	27	theme	fatty	728:732	arg1	SCFA					740:743	SCFA	740:743	SCFA	740:743	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	6	27	theme	fatty	728:732	arg1	acid					734:737	fatty acid	728:737	the short-chain fatty acid (SCFA) level	712:750	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	1	28	theme	health-promoting	185:200	arg1	functions					202:210	various health-promoting functions	177:210	various health-promoting functions	177:210	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	0	29	theme	bisporus	9:16	arg1	Polysaccharides					18:32	Agaricus bisporus Polysaccharides	0:32	Agaricus bisporus Polysaccharides	0:32	Agaricus bisporus Polysaccharides Ameliorates Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota.
36673515	3	30	from	diversity	391:399	arg1	mice					464:467	D-galactose-induced aging mice	438:467	D-galactose-induced aging mice	438:467	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	5	31	theme	oxidative	593:601	arg1	stress					603:608	oxidative stress	593:608	oxidative stress	593:608	It also alleviated oxidative stress and decreased the pro-inflammatory cytokine levels in the brain.
36673515	6	32	theme	short-chain	716:726	arg1	level					746:750	the short-chain fatty acid (SCFA) level	712:750	the short-chain fatty acid (SCFA) level	712:750	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	6	32	theme	short-chain	716:726	arg1	α-diversity					699:709	α-diversity	699:709	α-diversity	699:709	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	3	33	theme	microbiota	424:433	arg1	composition					405:415	composition	405:415	composition	405:415	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	3	33	theme	microbiota	424:433	arg1	diversity					391:399	diversity	391:399	diversity	391:399	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	0	34	theme	Agaricus	0:7	arg1	Polysaccharides					18:32	Agaricus bisporus Polysaccharides	0:32	Agaricus bisporus Polysaccharides	0:32	Agaricus bisporus Polysaccharides Ameliorates Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota.
36673515	9	35	theme	SCFA	1267:1270	arg1	levels					1272:1277	SCFA levels	1267:1277	SCFA levels	1267:1277	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	36	theme	strong	1197:1202	arg1	correlation					1204:1214	a strong correlation	1195:1214	a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter	1195:1377	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	37	from	improvements	1239:1250	arg1	levels					1272:1277	SCFA levels	1267:1277	SCFA levels	1267:1277	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	4	38	theme	mice	568:571	arg1	activity					507:514	locomotor activity	497:514	locomotor activity	497:514	WMP significantly improved locomotor activity and the spatial and recognition memory of the aging mice.
36673515	4	38	theme	mice	568:571	arg1	memory					548:553	the spatial and recognition memory	520:553	memory	548:553	WMP significantly improved locomotor activity and the spatial and recognition memory of the aging mice.
36673515	8	39	theme	greater	1053:1059	arg1	impact					1061:1066	a greater impact	1051:1066	a greater impact on the metabolism of carbon	1051:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	39	theme	greater	1053:1059	arg1	acid					1103:1106	fatty acid	1097:1106	fatty acid	1097:1106	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	39	theme	greater	1053:1059	arg1	acid					1118:1121	amino acid	1112:1121	amino acid	1112:1121	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	6	40	theme	genera	784:789	arg1	level					746:750	the short-chain fatty acid (SCFA) level	712:750	the short-chain fatty acid (SCFA) level	712:750	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	6	40	theme	genera	784:789	arg1	abundance					760:768	the abundance	756:768	the abundance of beneficial genera, such as Bacteroides and Parabacteroides	756:830	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	6	40	theme	genera	784:789	arg1	α-diversity					699:709	α-diversity	699:709	α-diversity	699:709	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	8	41	theme	fatty	1097:1101	arg1	acid					1103:1106	fatty acid	1097:1106	fatty acid	1097:1106	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	41	theme	fatty	1097:1101	arg1	impact					1061:1066	a greater impact	1051:1066	a greater impact on the metabolism of carbon	1051:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	5	42	from	levels	654:659	arg1	brain					668:672	the brain	664:672	the brain	664:672	It also alleviated oxidative stress and decreased the pro-inflammatory cytokine levels in the brain.
36673515	7	43	theme	&amp	953:956	arg1	enrichments					922:932	the enrichments	918:932	the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris	918:996	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	8	44	theme	PPAR	1148:1151	arg1	pathway					1163:1169	the MAPK and PPAR signaling pathway	1135:1169	pathway	1163:1169	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	3	45	theme	D-galactose-induced	438:456	arg1	mice					464:467	D-galactose-induced aging mice	438:467	D-galactose-induced aging mice	438:467	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	0	46	theme	Behavioural	46:56	arg1	Deficits					58:65	Behavioural Deficits	46:65	Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota	46:127	Agaricus bisporus Polysaccharides Ameliorates Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota.
36673515	6	47	theme	beneficial	773:782	arg1	genera					784:789	beneficial genera	773:789	beneficial genera	773:789	Moreover, WMP increased α-diversity, the short-chain fatty acid (SCFA) level and the abundance of beneficial genera, such as Bacteroides and Parabacteroides.
36673515	10	48	theme	specific	1492:1499	arg1	species					1501:1507	specific species	1492:1507	specific species	1492:1507	Therefore, WMP might be suitable as a functional foods to prevent or delay aging via the directed enrichment of specific species in Bacteroides.
36673515	0	49	theme	D-Galactose-Induced	70:88	arg1	Mice					96:99	D-Galactose-Induced Aging Mice	70:99	D-Galactose-Induced Aging Mice	70:99	Agaricus bisporus Polysaccharides Ameliorates Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota.
36673515	10	50	from	enrichment	1478:1487	arg1	Bacteroides					1512:1522	Bacteroides	1512:1522	Bacteroides	1512:1522	Therefore, WMP might be suitable as a functional foods to prevent or delay aging via the directed enrichment of specific species in Bacteroides.
36673515	1	51	theme	White	130:134	arg1	WMP					168:170	WMP	168:170	WMP	168:170	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	1	51	theme	White	130:134	arg1	polysaccharide					152:165	White button mushroom polysaccharide	130:165	White button mushroom polysaccharide (WMP)	130:171	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	8	52	theme	signaling	1153:1161	arg1	pathway					1163:1169	the MAPK and PPAR signaling pathway	1135:1169	pathway	1163:1169	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	4	53	theme	aging	562:566	arg1	mice					568:571	the aging mice	558:571	the aging mice	558:571	WMP significantly improved locomotor activity and the spatial and recognition memory of the aging mice.
36673515	4	54	theme	spatial	524:530	arg1	memory					548:553	the spatial and recognition memory	520:553	memory	548:553	WMP significantly improved locomotor activity and the spatial and recognition memory of the aging mice.
36673515	7	55	from	effect	847:852	arg1	Bacteroides					857:867	Bacteroides	857:867	Bacteroides	857:867	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	1	56	theme	button	136:141	arg1	WMP					168:170	WMP	168:170	WMP	168:170	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	1	56	theme	button	136:141	arg1	polysaccharide					152:165	White button mushroom polysaccharide	130:165	White button mushroom polysaccharide (WMP)	130:171	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	8	57	theme	carbon	1089:1094	arg1	metabolism					1075:1084	the metabolism	1071:1084	the metabolism of carbon	1071:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	7	58	theme	stercorirosoris	982:996	arg1	enrichments					922:932	the enrichments	918:932	the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris	918:996	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	1	59	theme	mushroom	143:150	arg1	WMP					168:170	WMP	168:170	WMP	168:170	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	1	59	theme	mushroom	143:150	arg1	polysaccharide					152:165	White button mushroom polysaccharide	130:165	White button mushroom polysaccharide (WMP)	130:171	White button mushroom polysaccharide (WMP) has various health-promoting functions.
36673515	8	60	from	MAPK	1139:1142	arg1	metabolism					1075:1084	the metabolism	1071:1084	the metabolism of carbon	1071:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	9	61	theme	behavioral	1228:1237	arg1	improvements					1239:1250	the behavioral improvements and changes	1224:1262	improvements	1239:1250	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	7	62	theme	sartorii	966:973	arg1	enrichments					922:932	the enrichments	918:932	the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris	918:996	Moreover, its effect on Bacteroides at the species level was further determined, and the enrichments of B. acidifaciens,&amp;nbsp;B. sartorii and B. stercorirosoris were found.
36673515	8	63	contain	had	1047:1049	arg2	MAPK					1139:1142	the MAPK and PPAR signaling pathway	1135:1169	MAPK	1139:1142	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	63	contain	had	1047:1049	arg2	acid					1118:1121	amino acid	1112:1121	amino acid	1112:1121	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	63	contain	had	1047:1049	arg2	acid					1103:1106	fatty acid	1097:1106	fatty acid	1097:1106	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	63	contain	had	1047:1049	arg1	WMP					1043:1045	WMP	1043:1045	WMP	1043:1045	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	8	63	contain	had	1047:1049	arg2	impact					1061:1066	a greater impact	1051:1066	a greater impact on the metabolism of carbon	1051:1094	A PICRUSt analysis revealed that WMP had a greater impact on the metabolism of carbon, fatty acid and amino acid, as well as the MAPK and PPAR signaling pathway.
36673515	3	64	theme	anti-aging	341:350	arg1	capacity					352:359	the anti-aging capacity	337:359	the anti-aging capacity of WMP	337:366	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	3	65	from	capacity	352:359	arg1	composition					405:415	composition	405:415	composition	405:415	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	3	65	from	capacity	352:359	arg1	diversity					391:399	diversity	391:399	diversity	391:399	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	3	66	theme	aging	458:462	arg1	mice					464:467	D-galactose-induced aging mice	438:467	D-galactose-induced aging mice	438:467	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	9	67	theme	Mucispirillum	1330:1342	arg1	changes					1256:1262	the behavioral improvements and changes	1224:1262	changes	1256:1262	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	67	theme	Mucispirillum	1330:1342	arg1	abundance					1287:1295	the abundance	1283:1295	the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter	1283:1377	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	67	theme	Mucispirillum	1330:1342	arg1	improvements					1239:1250	the behavioral improvements and changes	1224:1262	improvements	1239:1250	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	0	68	theme	Aging	90:94	arg1	Mice					96:99	D-Galactose-Induced Aging Mice	70:99	D-Galactose-Induced Aging Mice	70:99	Agaricus bisporus Polysaccharides Ameliorates Behavioural Deficits in D-Galactose-Induced Aging Mice: Mediated by Gut Microbiota.
36673515	3	69	theme	gut	420:422	arg1	microbiota					424:433	gut microbiota	420:433	gut microbiota	420:433	Therefore, this study evaluated the anti-aging capacity of WMP and its effects on the diversity and composition of gut microbiota in D-galactose-induced aging mice.
36673515	2	70	theme	gut	262:264	arg1	microbiota					266:275	gut microbiota	262:275	gut microbiota	262:275	However, whether these functions are mediated by gut microbiota has not been well explored.
36673515	9	71	theme	Parabacteroides	1313:1327	arg1	changes					1256:1262	the behavioral improvements and changes	1224:1262	changes	1256:1262	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	71	theme	Parabacteroides	1313:1327	arg1	abundance					1287:1295	the abundance	1283:1295	the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter	1283:1377	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	71	theme	Parabacteroides	1313:1327	arg1	improvements					1239:1250	the behavioral improvements and changes	1224:1262	improvements	1239:1250	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
36673515	9	72	from	changes	1256:1262	arg1	levels					1272:1277	SCFA levels	1267:1277	SCFA levels	1267:1277	In addition, there was a strong correlation between the behavioral improvements and changes in SCFA levels and the abundance of Bacteroides, Parabacteroides, Mucispirillum and Desulfovibrio and Helicobacter.
37358876	10	0	theme	organic	1425:1431	arg1	acids					1433:1437	other organic acids	1419:1437	other organic acids	1419:1437	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	7	1	theme	β-configuration	925:939	arg1	residue					951:957	the corresponding β-configuration galactose residue	907:957	the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a	907:996	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	4	2	dep	RESULTS	458:464	arg1	RESULTS					458:464	RESULTS APS2a and HAPS2a	458:481	RESULTS APS2a and HAPS2a	458:481	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	4	2	dep	RESULTS	458:464	arg1	HAPS2a					476:481	HAPS2a	476:481	HAPS2a	476:481	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	4	2	dep	RESULTS	458:464	arg1	APS2a					466:470	APS2a	466:470	APS2a	466:470	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	5	3	theme	acidic	587:592	arg1	heteropolysaccharides					594:614	highly branched acidic heteropolysaccharides	571:614	highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds	571:678	Both of them were highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds.
37358876	11	4	theme	different	1572:1580	arg1	activities					1592:1601	different probiotic activities	1572:1601	different probiotic activities	1572:1601	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	2	5	theme	dosage	285:290	arg1	form					292:295	a dosage form	283:295	a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product	283:405	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	2	5	theme	dosage	285:290	arg1	Astragalus					269:278	Honey-processed Astragalus	253:278	Honey-processed Astragalus	253:278	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	9	6	from	changes	1342:1348	arg1	composition					1374:1384	their monosaccharide composition	1353:1384	their monosaccharide composition	1353:1384	After degradation, the molecular weights of HAPS2a and APS2a decreased with the changes in their monosaccharide composition.
37358876	7	7	theme	HAPS2a	1143:1148	arg1	chain					1134:1138	the side chain	1125:1138	the side chain of HAPS2a	1125:1148	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	2	8	theme	Radix	310:314	arg1	form					292:295	a dosage form	283:295	a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product	283:405	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	2	8	theme	Radix	310:314	arg1	Astragalus					269:278	Honey-processed Astragalus	253:278	Honey-processed Astragalus	253:278	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	7	9	theme	APS2a	884:888	arg1	backbone					872:879	the backbone	868:879	the backbone of APS2a	868:888	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	12	10	theme	dietary	1779:1785	arg1	supplement					1787:1796	dietary supplement	1779:1796	dietary supplement	1779:1796	Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement.
37358876	5	11	contain	containing	616:625	arg1	heteropolysaccharides					594:614	highly branched acidic heteropolysaccharides	571:614	highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds	571:678	Both of them were highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds.
37358876	5	11	contain	containing	616:625	arg2	bonds					674:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	Both of them were highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds.
37358876	1	12	theme	edible	211:216	arg1	plant					229:233	edible homologous plant	211:233	edible homologous plant	211:233	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	0	13	from	processing	66:75	arg1	microbiota					114:123	human gut microbiota	104:123	human gut microbiota	104:123	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	12	14	from	immunopotentiator	1741:1757	arg1	foods					1770:1774	healthy foods	1762:1774	healthy foods	1762:1774	Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement.
37358876	12	14	from	immunopotentiator	1741:1757	arg1	supplement					1787:1796	dietary supplement	1779:1796	dietary supplement	1779:1796	Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement.
37358876	7	15	theme	neutral	1097:1103	arg1	T-α-Galp					1113:1120	the corresponding neutral residue T-α-Galp	1079:1120	the corresponding neutral residue T-α-Galp in the side chain of HAPS2a	1079:1148	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	2	16	theme	Honey-processed	253:267	arg1	form					292:295	a dosage form	283:295	a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product	283:405	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	2	16	theme	Honey-processed	253:267	arg1	Astragalus					269:278	Honey-processed Astragalus	253:278	Honey-processed Astragalus	253:278	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	12	17	used	used	1730:1733	arg2	immunopotentiator					1741:1757	an immunopotentiator	1738:1757	an immunopotentiator in healthy foods or dietary supplement	1738:1796	Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement.
37358876	12	17	used	used	1730:1733	arg2	them					1707:1710	them	1707:1710	them	1707:1710	Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement.
37358876	12	17	used	used	1730:1733	arg2	Both					1699:1702	Both	1699:1702	Both	1699:1702	Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement.
37358876	12	18	theme	healthy	1762:1768	arg1	foods					1770:1774	healthy foods	1762:1774	healthy foods	1762:1774	Both of them might be possibly used as an immunopotentiator in healthy foods or dietary supplement.
37358876	11	19	theme	high-molecular-weight	1507:1527	arg1	polysaccharides					1529:1543	Two novel high-molecular-weight polysaccharides	1497:1543	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a	1485:1566	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	9	20	theme	HAPS2a	1306:1311	arg1	weights					1295:1301	the molecular weights	1281:1301	the molecular weights of HAPS2a and APS2a	1281:1321	After degradation, the molecular weights of HAPS2a and APS2a decreased with the changes in their monosaccharide composition.
37358876	10	21	theme	APS2a	1472:1476	arg1	group					1478:1482	APS2a group	1472:1482	APS2a group	1472:1482	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	7	22	from	T-α-GalpA	1026:1034	arg1	sidechain					1043:1051	the sidechain	1039:1051	the sidechain of APS2a	1039:1060	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	9	23	theme	APS2a	1317:1321	arg1	weights					1295:1301	the molecular weights	1281:1301	the molecular weights of HAPS2a and APS2a	1281:1321	After degradation, the molecular weights of HAPS2a and APS2a decreased with the changes in their monosaccharide composition.
37358876	5	24	theme	ɑ-configuration	627:641	arg1	bonds					674:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	Both of them were highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds.
37358876	10	25	theme	SCFAs	1409:1413	arg1	contents					1391:1398	The contents	1387:1398	The contents of total SCFAs and other organic acids in HAPS2a group	1387:1453	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	10	25	theme	SCFAs	1409:1413	arg1	higher					1460:1465	higher	1460:1465	higher	1460:1465	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	7	26	theme	acid	1013:1016	arg1	residue					1018:1024	the uronic acid residue	1002:1024	the uronic acid residue T-α-GalpA in the sidechain of APS2a	1002:1060	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	3	27	theme	main	434:437	arg1	components					446:455	their main active components	428:455	their main active components	428:455	Polysaccharides are their main active components.
37358876	3	27	theme	main	434:437	arg1	Polysaccharides					408:422	Polysaccharides	408:422	Polysaccharides	408:422	Polysaccharides are their main active components.
37358876	0	28	theme	gut	110:112	arg1	microbiota					114:123	human gut microbiota	104:123	human gut microbiota	104:123	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	5	29	theme	branched	578:585	arg1	heteropolysaccharides					594:614	highly branched acidic heteropolysaccharides	571:614	highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds	571:678	Both of them were highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds.
37358876	1	30	dep	known	159:163	arg1	also					154:157	also	154:157	also	154:157	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	2	31	theme	Qi	383:384	arg1	efficacy					361:368	better efficacy	354:368	better efficacy of tonifying Qi	354:384	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	0	32	theme	Structural	0:9	arg1	changes					11:17	Structural changes	0:17	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.	0:124	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	7	33	theme	HAPS2a	991:996	arg1	backbone					979:986	the backbone	975:986	the backbone of HAPS2a	975:996	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	11	34	dep	had	1568:1570	arg1	due					1628:1630	due	1628:1630	due	1628:1630	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	6	35	theme	HAPS2a	733:738	arg1	dimension					720:728	the molecular dimension	706:728	the molecular dimension	706:728	The molecular weight and the molecular dimension of HAPS2a decreased and the GalA contained in APS2a was converted to Gal in HAPS2a.
37358876	6	35	theme	HAPS2a	733:738	arg1	weight					695:700	The molecular weight	681:700	The molecular weight	681:700	The molecular weight and the molecular dimension of HAPS2a decreased and the GalA contained in APS2a was converted to Gal in HAPS2a.
37358876	7	36	theme	residue	844:850	arg1	1,3,4-α-Galp					852:863	The α-configuration galactose residue 1,3,4-α-Galp	814:863	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a	814:888	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	7	37	theme	α-configuration	818:832	arg1	1,3,4-α-Galp					852:863	The α-configuration galactose residue 1,3,4-α-Galp	814:863	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a	814:888	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	11	38	contain	had	1568:1570	arg1	CONCLUSIONS					1485:1495	CONCLUSIONS	1485:1495	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a	1485:1566	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	11	38	contain	had	1568:1570	arg2	activities					1592:1601	different probiotic activities	1572:1601	different probiotic activities	1572:1601	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	4	39	theme	honey-processed	525:539	arg1	Astragulus					541:550	honey-processed Astragulus	525:550	honey-processed Astragulus	525:550	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	4	40	attach	isolated	496:503	arg2	APS2a					466:470	APS2a	466:470	APS2a	466:470	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	4	40	attach	isolated	496:503	arg2	RESULTS					458:464	RESULTS APS2a and HAPS2a	458:481	RESULTS APS2a and HAPS2a	458:481	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	4	40	attach	isolated	496:503	arg1	Astragulus					510:519	Astragulus	510:519	Astragulus	510:519	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	4	40	attach	isolated	496:503	arg1	Astragulus					541:550	honey-processed Astragulus	525:550	honey-processed Astragulus	525:550	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	4	40	attach	isolated	496:503	arg2	HAPS2a					476:481	HAPS2a	476:481	HAPS2a	476:481	RESULTS APS2a and HAPS2a were firstly isolated from Astragulus and honey-processed Astragulus.
37358876	1	41	theme	homologous	218:227	arg1	plant					229:233	edible homologous plant	211:233	edible homologous plant	211:233	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	7	42	from	T-α-Galp	1113:1120	arg1	chain					1134:1138	the side chain	1125:1138	the side chain of HAPS2a	1125:1148	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	2	43	theme	better	354:359	arg1	efficacy					361:368	better efficacy	354:368	better efficacy of tonifying Qi	354:384	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	8	44	theme	probiotic	1201:1209	arg1	effects					1211:1217	better probiotic effects	1194:1217	better probiotic effects	1194:1217	Bioactivity results showed that HAPS2a had better probiotic effects on BO, BT, BL, and LGG strains than APS2a.
37358876	11	45	theme	honey	1681:1685	arg1	processing					1687:1696	honey processing	1681:1696	honey processing	1681:1696	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	10	46	theme	other	1419:1423	arg1	acids					1433:1437	other organic acids	1419:1437	other organic acids	1419:1437	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	7	47	theme	galactose	941:949	arg1	residue					951:957	the corresponding β-configuration galactose residue	907:957	the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a	907:996	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	11	48	theme	probiotic	1582:1590	arg1	activities					1592:1601	different probiotic activities	1572:1601	different probiotic activities	1572:1601	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	7	49	theme	corresponding	911:923	arg1	residue					951:957	the corresponding β-configuration galactose residue	907:957	the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a	907:996	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	7	50	from	1,3,4-α-Galp	852:863	arg1	backbone					872:879	the backbone	868:879	the backbone of APS2a	868:888	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	10	51	theme	HAPS2a	1442:1447	arg1	group					1449:1453	HAPS2a group	1442:1453	HAPS2a group	1442:1453	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	7	52	theme	side	1129:1132	arg1	chain					1134:1138	the side chain	1125:1138	the side chain of HAPS2a	1125:1148	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	10	53	theme	acids	1433:1437	arg1	contents					1391:1398	The contents	1387:1398	The contents of total SCFAs and other organic acids in HAPS2a group	1387:1453	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	10	53	theme	acids	1433:1437	arg1	higher					1460:1465	higher	1460:1465	higher	1460:1465	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	8	54	contain	had	1190:1192	arg2	effects					1211:1217	better probiotic effects	1194:1217	better probiotic effects	1194:1217	Bioactivity results showed that HAPS2a had better probiotic effects on BO, BT, BL, and LGG strains than APS2a.
37358876	8	54	contain	had	1190:1192	arg1	HAPS2a					1183:1188	HAPS2a	1183:1188	HAPS2a	1183:1188	Bioactivity results showed that HAPS2a had better probiotic effects on BO, BT, BL, and LGG strains than APS2a.
37358876	1	55	theme	traditional	185:195	arg1	medicinal					197:205	a traditional medicinal	183:205	a traditional medicinal	183:205	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	1	55	theme	traditional	185:195	arg1	Radix					147:151	Radix	147:151	Radix	147:151	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	0	56	from	bioactivities	87:99	arg1	microbiota					114:123	human gut microbiota	104:123	human gut microbiota	104:123	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	8	57	theme	LGG	1238:1240	arg1	strains					1242:1248	LGG strains	1238:1248	LGG strains	1238:1248	Bioactivity results showed that HAPS2a had better probiotic effects on BO, BT, BL, and LGG strains than APS2a.
37358876	7	58	theme	residue	1105:1111	arg1	T-α-Galp					1113:1120	the corresponding neutral residue T-α-Galp	1079:1120	the corresponding neutral residue T-α-Galp in the side chain of HAPS2a	1079:1148	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	6	59	theme	molecular	710:718	arg1	dimension					720:728	the molecular dimension	706:728	the molecular dimension	706:728	The molecular weight and the molecular dimension of HAPS2a decreased and the GalA contained in APS2a was converted to Gal in HAPS2a.
37358876	7	60	theme	corresponding	1083:1095	arg1	T-α-Galp					1113:1120	the corresponding neutral residue T-α-Galp	1079:1120	the corresponding neutral residue T-α-Galp in the side chain of HAPS2a	1079:1148	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	9	61	theme	molecular	1285:1293	arg1	weights					1295:1301	the molecular weights	1281:1301	the molecular weights of HAPS2a and APS2a	1281:1321	After degradation, the molecular weights of HAPS2a and APS2a decreased with the changes in their monosaccharide composition.
37358876	11	62	theme	novel	1501:1505	arg1	polysaccharides					1529:1543	Two novel high-molecular-weight polysaccharides	1497:1543	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a	1485:1566	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	7	63	theme	APS2a	1056:1060	arg1	sidechain					1043:1051	the sidechain	1039:1051	the sidechain of APS2a	1039:1060	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	11	64	dep	CONCLUSIONS	1485:1495	arg1	polysaccharides					1529:1543	Two novel high-molecular-weight polysaccharides	1497:1543	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a	1485:1566	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	6	65	theme	molecular	685:693	arg1	weight					695:700	The molecular weight	681:700	The molecular weight	681:700	The molecular weight and the molecular dimension of HAPS2a decreased and the GalA contained in APS2a was converted to Gal in HAPS2a.
37358876	0	66	from	changes	11:17	arg1	Astragulus					43:52	Astragulus	43:52	Astragulus	43:52	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	0	67	theme	human	104:108	arg1	microbiota					114:123	human gut microbiota	104:123	human gut microbiota	104:123	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	5	68	theme	β-configuration	647:661	arg1	bonds					674:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	Both of them were highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds.
37358876	1	69	dep	Radix	147:151	arg1	known					159:163	known	159:163	known as Astragulus	159:177	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	5	70	theme	glycosidic	663:672	arg1	bonds					674:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	ɑ-configuration and β-configuration glycosidic bonds	627:678	Both of them were highly branched acidic heteropolysaccharides containing ɑ-configuration and β-configuration glycosidic bonds.
37358876	0	71	from	Astragulus	43:52	arg1	polysaccharides					22:36	polysaccharides	22:36	polysaccharides from Astragulus	22:52	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	0	71	from	Astragulus	43:52	arg1	changes					11:17	Structural changes	0:17	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.	0:124	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	7	72	from	1,3,4-β-Galp	959:970	arg1	backbone					979:986	the backbone	975:986	the backbone of HAPS2a	975:996	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	3	73	theme	active	439:444	arg1	components					446:455	their main active components	428:455	their main active components	428:455	Polysaccharides are their main active components.
37358876	3	73	theme	active	439:444	arg1	Polysaccharides					408:422	Polysaccharides	408:422	Polysaccharides	408:422	Polysaccharides are their main active components.
37358876	7	74	theme	uronic	1006:1011	arg1	residue					1018:1024	the uronic acid residue	1002:1024	the uronic acid residue T-α-GalpA in the sidechain of APS2a	1002:1060	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	2	75	theme	tonifying	373:381	arg1	Qi					383:384	tonifying Qi	373:384	tonifying Qi	373:384	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	0	76	theme	polysaccharides	22:36	arg1	changes					11:17	Structural changes	0:17	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.	0:124	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	9	77	theme	monosaccharide	1359:1372	arg1	composition					1374:1384	their monosaccharide composition	1353:1384	their monosaccharide composition	1353:1384	After degradation, the molecular weights of HAPS2a and APS2a decreased with the changes in their monosaccharide composition.
37358876	7	78	theme	residue	1018:1024	arg1	T-α-GalpA					1026:1034	the uronic acid residue T-α-GalpA	1002:1034	the uronic acid residue T-α-GalpA in the sidechain of APS2a	1002:1060	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	10	79	from	contents	1391:1398	arg1	group					1449:1453	HAPS2a group	1442:1453	HAPS2a group	1442:1453	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	8	80	theme	Bioactivity	1151:1161	arg1	results					1163:1169	Bioactivity results	1151:1169	Bioactivity results	1151:1169	Bioactivity results showed that HAPS2a had better probiotic effects on BO, BT, BL, and LGG strains than APS2a.
37358876	2	81	theme	raw	395:397	arg1	product					399:405	the raw product	391:405	the raw product	391:405	Honey-processed Astragalus is a dosage form of Astragali Radix processed with honey, which exhibited better efficacy of tonifying Qi than the raw product.
37358876	0	82	theme	honey	60:64	arg1	processing					66:75	honey processing	60:75	honey processing	60:75	Structural changes of polysaccharides from Astragulus after honey processing and their bioactivities on human gut microbiota.
37358876	7	83	theme	galactose	834:842	arg1	1,3,4-α-Galp					852:863	The α-configuration galactose residue 1,3,4-α-Galp	814:863	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a	814:888	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	1	84	dep	BACKGROUND	126:135	arg1	medicinal					197:205	a traditional medicinal	183:205	a traditional medicinal	183:205	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	1	84	dep	BACKGROUND	126:135	arg1	Radix					147:151	Radix	147:151	Radix	147:151	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	1	84	dep	BACKGROUND	126:135	arg1	plant					229:233	edible homologous plant	211:233	edible homologous plant	211:233	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
37358876	10	85	theme	total	1403:1407	arg1	SCFAs					1409:1413	total SCFAs	1403:1413	total SCFAs	1403:1413	The contents of total SCFAs and other organic acids in HAPS2a group were higher than APS2a group.
37358876	8	86	theme	better	1194:1199	arg1	effects					1211:1217	better probiotic effects	1194:1217	better probiotic effects	1194:1217	Bioactivity results showed that HAPS2a had better probiotic effects on BO, BT, BL, and LGG strains than APS2a.
37358876	11	87	theme	structural	1641:1650	arg1	differences					1652:1662	their structural differences	1635:1662	their structural differences before and after honey processing	1635:1696	CONCLUSIONS Two novel high-molecular-weight polysaccharides named APS2a and HAPS2a had different probiotic activities in vitro, which might be due to their structural differences before and after honey processing.
37358876	7	88	theme	residue	951:957	arg1	1,3,4-β-Galp					959:970	the corresponding β-configuration galactose residue 1,3,4-β-Galp	907:970	the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a	907:996	The α-configuration galactose residue 1,3,4-α-Galp in the backbone of APS2a was converted to the corresponding β-configuration galactose residue 1,3,4-β-Galp in the backbone of HAPS2a and the uronic acid residue T-α-GalpA in the sidechain of APS2a was converted to the corresponding neutral residue T-α-Galp in the side chain of HAPS2a.
37358876	1	89	theme	tonifying	239:247	arg1	Qi					249:250	tonifying Qi	239:250	tonifying Qi	239:250	BACKGROUND Astragali Radix (also known as Astragulus) is a traditional medicinal and edible homologous plant for tonifying Qi.
35315841	8	0	used	occupied	1096:1103	arg2	molecules					1074:1082	lipid-like molecules	1063:1082	lipid-like molecules	1063:1082	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	8	0	used	occupied	1096:1103	arg2	lipid					1053:1057	lipid	1053:1057	lipid	1053:1057	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	9	1	theme	phospholipid	1292:1303	arg1	metabolism					1305:1314	phospholipid metabolism	1292:1314	phospholipid metabolism	1292:1314	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	9	1	theme	phospholipid	1292:1303	arg1	pathways					1271:1278	the metabolic pathways	1257:1278	the metabolic pathways	1257:1278	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	2	2	theme	ppm	440:442	arg1	ε-polylysine					444:455	150 ppm ε-polylysine	436:455	150 ppm ε-polylysine	436:455	A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine.
35315841	8	3	theme	metabolite	1020:1029	arg1	profiling					1031:1039	The metabolite profiling	1016:1039	The metabolite profiling	1016:1039	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	0	4	with	mice	86:89	arg1	ε-polylysine					104:115	dietary ε-polylysine	96:115	dietary ε-polylysine	96:115	Multi-omics reveals host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine.
35315841	7	5	theme	genera	853:858	arg1	presence					841:848	the presence	837:848	the presence of genera and species	837:870	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	7	6	theme	amino	946:950	arg1	acids					952:956	amino acids	946:956	amino acids	946:956	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	0	7	theme	dietary	96:102	arg1	ε-polylysine					104:115	dietary ε-polylysine	96:115	dietary ε-polylysine	96:115	Multi-omics reveals host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine.
35315841	9	8	theme	metabolic	1261:1269	arg1	metabolism					1305:1314	phospholipid metabolism	1292:1314	phospholipid metabolism	1292:1314	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	9	8	theme	metabolic	1261:1269	arg1	pathways					1271:1278	the metabolic pathways	1257:1278	the metabolic pathways	1257:1278	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	10	9	theme	greater	1402:1408	arg1	effect					1410:1415	a greater effect	1400:1415	a greater effect	1400:1415	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	6	10	theme	phyla	789:793	arg1	microbiota					764:773	microbiota	764:773	microbiota	764:773	The richness of the microbiota and number of phyla increased with age.
35315841	6	10	theme	phyla	789:793	arg1	number					779:784	number	779:784	number	779:784	The richness of the microbiota and number of phyla increased with age.
35315841	4	11	theme	metabolomic	564:574	arg1	analyses					590:597	metabolomic and proteomic analyses	564:597	metabolomic and proteomic analyses	564:597	Liver and plasma samples were collected for metabolomic and proteomic analyses.
35315841	5	12	theme	types	708:712	arg1	presence					688:695	the presence	684:695	the presence of certain types of intestinal microorganisms	684:741	The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms.
35315841	5	13	theme	body	651:654	arg1	weight					656:661	the body weight	647:661	the body weight of mice	647:669	The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms.
35315841	10	14	contain	had	1396:1398	arg1	ε-polylysine					1340:1351	dietary ε-polylysine	1332:1351	dietary ε-polylysine	1332:1351	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	10	14	contain	had	1396:1398	arg2	effect					1410:1415	a greater effect	1400:1415	a greater effect	1400:1415	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	8	15	theme	plasma	1129:1134	arg1	metabolites					1146:1156	plasma and liver metabolites	1129:1156	plasma and liver metabolites	1129:1156	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	10	16	theme	nutrients	1437:1445	arg1	anabolism					1424:1432	the anabolism	1420:1432	the anabolism of nutrients in adult mice	1420:1459	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	6	17	theme	number	779:784	arg1	richness					748:755	The richness	744:755	The richness of the microbiota and number of phyla	744:793	The richness of the microbiota and number of phyla increased with age.
35315841	3	18	theme	gut	491:493	arg1	identification					504:517	gut bacteria identification	491:517	gut bacteria identification	491:517	Fecal samples were collected for gut bacteria identification.
35315841	1	19	theme	dietary	162:168	arg1	supplementation					170:184	dietary supplementation	162:184	dietary supplementation of ε-polylysine	162:200	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	5	20	theme	intestinal	717:726	arg1	microorganisms					728:741	intestinal microorganisms	717:741	intestinal microorganisms	717:741	The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms.
35315841	9	21	theme	key	1200:1202	arg1	role					1204:1207	the key role	1196:1207	the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism	1196:1314	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	3	22	theme	bacteria	495:502	arg1	identification					504:517	gut bacteria identification	491:517	gut bacteria identification	491:517	Fecal samples were collected for gut bacteria identification.
35315841	1	23	theme	supplementation	170:184	arg1	influence					149:157	the influence	145:157	the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis	145:314	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	8	24	theme	liver	1140:1144	arg1	metabolites					1146:1156	plasma and liver metabolites	1129:1156	plasma and liver metabolites	1129:1156	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	2	25	with	diet	400:403	arg1	ε-polylysine					444:455	150 ppm ε-polylysine	436:455	150 ppm ε-polylysine	436:455	A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine.
35315841	6	26	theme	microbiota	764:773	arg1	richness					748:755	The richness	744:755	The richness of the microbiota and number of phyla	744:793	The richness of the microbiota and number of phyla increased with age.
35315841	6	27	dep	microbiota	764:773	arg1	the					760:762	the	760:762	the	760:762	The richness of the microbiota and number of phyla increased with age.
35315841	1	28	theme	ε-polylysine	189:200	arg1	supplementation					170:184	dietary supplementation	162:184	dietary supplementation of ε-polylysine	162:200	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	8	29	dep	lipid	1053:1057	arg1	metabolites					1084:1094	metabolites	1084:1094	metabolites	1084:1094	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	4	30	theme	Liver	520:524	arg1	samples					537:543	Liver and plasma samples	520:543	Liver and plasma samples	520:543	Liver and plasma samples were collected for metabolomic and proteomic analyses.
35315841	7	31	theme	vitamins	1006:1013	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	10	32	theme	mice	1386:1389	arg1	immunity					1366:1373	the immunity	1362:1373	the immunity of growing mice	1362:1389	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	1	33	from	influence	149:157	arg1	metabolism					242:251	the gut microbiota and host nutrient metabolism	205:251	metabolism	242:251	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	1	33	from	influence	149:157	arg1	microbiota					213:222	the gut microbiota and host nutrient metabolism	205:251	microbiota	213:222	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	4	34	theme	plasma	530:535	arg1	samples					537:543	Liver and plasma samples	520:543	Liver and plasma samples	520:543	Liver and plasma samples were collected for metabolomic and proteomic analyses.
35315841	0	35	theme	host	20:23	arg1	metabolism					25:34	host metabolism	20:34	host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine	20:115	Multi-omics reveals host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine.
35315841	10	36	theme	growing	1378:1384	arg1	mice					1386:1389	growing mice	1378:1389	growing mice	1378:1389	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	3	37	theme	Fecal	458:462	arg1	samples					464:470	Fecal samples	458:470	Fecal samples	458:470	Fecal samples were collected for gut bacteria identification.
35315841	8	38	theme	percent	1118:1124	arg1	majority					1109:1116	the majority percent	1105:1124	the majority percent of plasma and liver metabolites	1105:1156	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	1	39	theme	multi-omics	295:305	arg1	analysis					307:314	multi-omics analysis	295:314	multi-omics analysis	295:314	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	10	40	theme	adult	1450:1454	arg1	mice					1456:1459	adult mice	1450:1459	adult mice	1450:1459	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	1	41	theme	gut	209:211	arg1	microbiota					213:222	the gut microbiota and host nutrient metabolism	205:251	microbiota	213:222	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	9	42	from	role	1204:1207	arg1	metabolism					1305:1314	phospholipid metabolism	1292:1314	phospholipid metabolism	1292:1314	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	9	42	from	role	1204:1207	arg1	pathways					1271:1278	the metabolic pathways	1257:1278	the metabolic pathways	1257:1278	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	2	43	theme	basal	394:398	arg1	AIN-76A					406:412	AIN-76A	406:412	AIN-76A	406:412	A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine.
35315841	2	43	theme	basal	394:398	arg1	diet					400:403	a basal diet	392:403	a basal diet (AIN-76A)	392:413	A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine.
35315841	8	44	theme	lipid-like	1063:1072	arg1	molecules					1074:1082	lipid-like molecules	1063:1082	lipid-like molecules	1063:1082	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	10	45	from	anabolism	1424:1432	arg1	mice					1456:1459	adult mice	1450:1459	adult mice	1450:1459	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	0	46	from	composition	71:81	arg1	mice					86:89	mice	86:89	mice with dietary ε-polylysine	86:115	Multi-omics reveals host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine.
35315841	0	47	theme	gut	56:58	arg1	composition					71:81	the gut microbiota composition	52:81	the gut microbiota composition in mice with dietary ε-polylysine	52:115	Multi-omics reveals host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine.
35315841	5	48	theme	certain	700:706	arg1	types					708:712	certain types	700:712	certain types of intestinal microorganisms	700:741	The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms.
35315841	7	49	theme	carbohydrates	967:979	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	2	50	theme	basal	420:424	arg1	diet					426:429	a basal diet	418:429	a basal diet with 150 ppm ε-polylysine	418:455	A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine.
35315841	7	51	theme	cofactors	991:999	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	9	52	theme	metabolites	1212:1222	arg1	role					1204:1207	the key role	1196:1207	the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism	1196:1314	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	2	53	theme	mice	331:334	arg1	total					319:323	A total	317:323	A total of 40 mice	317:334	A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine.
35315841	5	54	theme	mice	666:669	arg1	weight					656:661	the body weight	647:661	the body weight of mice	647:669	The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms.
35315841	7	55	theme	lipids	959:964	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	10	56	theme	dietary	1332:1338	arg1	ε-polylysine					1340:1351	dietary ε-polylysine	1332:1351	dietary ε-polylysine	1332:1351	In conclusion, dietary ε-polylysine improved the immunity of growing mice, and had a greater effect on the anabolism of nutrients in adult mice.
35315841	4	57	theme	proteomic	580:588	arg1	analyses					590:597	metabolomic and proteomic analyses	564:597	metabolomic and proteomic analyses	564:597	Liver and plasma samples were collected for metabolomic and proteomic analyses.
35315841	7	58	theme	glycans	982:988	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	9	59	theme	related	1228:1234	arg1	enzymes					1246:1252	related metabolic enzymes	1228:1252	related metabolic enzymes	1228:1252	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	2	60	with	diet	426:429	arg1	ε-polylysine					444:455	150 ppm ε-polylysine	436:455	150 ppm ε-polylysine	436:455	A total of 40 mice were randomly divided into two groups exposed to either a basal diet (AIN-76A) or a basal diet with 150 ppm ε-polylysine.
35315841	1	61	theme	host	228:231	arg1	metabolism					242:251	the gut microbiota and host nutrient metabolism	205:251	metabolism	242:251	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	5	62	theme	microorganisms	728:741	arg1	types					708:712	certain types	700:712	certain types of intestinal microorganisms	700:741	The results showed that ε-polylysine decreased the body weight of mice and affected the presence of certain types of intestinal microorganisms.
35315841	9	63	theme	metabolic	1236:1244	arg1	enzymes					1246:1252	related metabolic enzymes	1228:1252	related metabolic enzymes	1228:1252	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	7	64	theme	acids	952:956	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	7	65	theme	energy	928:933	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	1	66	theme	nutrient	233:240	arg1	metabolism					242:251	the gut microbiota and host nutrient metabolism	205:251	metabolism	242:251	This study aimed to assess the influence of dietary supplementation of ε-polylysine on the gut microbiota and host nutrient metabolism, which is not systematically discussed by multi-omics analysis.
35315841	0	67	theme	microbiota	60:69	arg1	composition					71:81	the gut microbiota composition	52:81	the gut microbiota composition in mice with dietary ε-polylysine	52:115	Multi-omics reveals host metabolism associated with the gut microbiota composition in mice with dietary ε-polylysine.
35315841	9	68	theme	enzymes	1246:1252	arg1	role					1204:1207	the key role	1196:1207	the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism	1196:1314	Additionally, ε-polylysine regulated the key role of metabolites and related metabolic enzymes in the metabolic pathways, especially phospholipid metabolism.
35315841	8	69	theme	metabolites	1146:1156	arg1	majority					1109:1116	the majority percent	1105:1124	the majority percent of plasma and liver metabolites	1105:1156	The metabolite profiling showed that lipid and lipid-like molecules metabolites occupied the majority percent of plasma and liver metabolites.
35315841	7	70	theme	nitrogen	936:943	arg1	metabolism					914:923	the metabolism	910:923	the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins	910:1013	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
35315841	7	71	theme	species	864:870	arg1	presence					841:848	the presence	837:848	the presence of genera and species	837:870	ε-Polylysine affected the presence of genera and species, and either regulated or took part in the metabolism of energy, nitrogen, amino acids, lipids, carbohydrates, glycans, cofactors, and vitamins.
37351508	12	0	theme	citrate	1777:1783	arg1	cycle					1785:1789	the citrate cycle	1773:1789	the citrate cycle	1773:1789	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	0	theme	citrate	1777:1783	arg1	metabolism					1761:1770	aromatic amino acid metabolism	1741:1770	aromatic amino acid metabolism	1741:1770	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	11	1	theme	mouse	1241:1245	arg1	model					1255:1259	the mouse colitis model	1237:1259	the mouse colitis model	1237:1259	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	7	2	theme	C57BL/6J	618:625	arg1	mice					627:630	C57BL/6J mice	618:630	C57BL/6J mice	618:630	Methods: C57BL/6J mice were given DSS (2.5%) in water and were treated with HSH and BHR (200 mg/kg/day) by gavage.
37351508	12	3	theme	aromatic	1741:1748	arg1	cycle					1785:1789	the citrate cycle	1773:1789	the citrate cycle	1773:1789	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	3	theme	aromatic	1741:1748	arg1	metabolism					1799:1808	purine metabolism	1792:1808	purine metabolism	1792:1808	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	3	theme	aromatic	1741:1748	arg1	metabolism					1822:1831	pyrimidine metabolism	1811:1831	pyrimidine metabolism	1811:1831	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	3	theme	aromatic	1741:1748	arg1	etc					1834:1836	etc	1834:1836	etc	1834:1836	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	3	theme	aromatic	1741:1748	arg1	metabolism					1761:1770	aromatic amino acid metabolism	1741:1770	aromatic amino acid metabolism	1741:1770	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	14	4	theme	g_Parabacteroides	2077:2093	arg1	abundances					2025:2034	the abundances	2021:2034	the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter	2021:2112	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	5	5	theme	systemic	419:426	arg1	effects					439:445	their systemic modulation effects	413:445	their systemic modulation effects	413:445	Pilat (BHR) showed noticeable effects on dextran sulfate sodium (DSS)-induced colitis, but their systemic modulation effects have not been fully revealed.
37351508	3	6	from	Dicks	275:279	arg1	Polysaccharides					236:250	Polysaccharides	236:250	Introduction: Polysaccharides from Grifola frondosa (Dicks.)	222:281	Introduction: Polysaccharides from Grifola frondosa (Dicks.)
37351508	12	7	theme	acid	1756:1759	arg1	cycle					1785:1789	the citrate cycle	1773:1789	the citrate cycle	1773:1789	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	7	theme	acid	1756:1759	arg1	metabolism					1799:1808	purine metabolism	1792:1808	purine metabolism	1792:1808	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	7	theme	acid	1756:1759	arg1	metabolism					1822:1831	pyrimidine metabolism	1811:1831	pyrimidine metabolism	1811:1831	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	7	theme	acid	1756:1759	arg1	etc					1834:1836	etc	1834:1836	etc	1834:1836	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	7	theme	acid	1756:1759	arg1	metabolism					1761:1770	aromatic amino acid metabolism	1741:1770	aromatic amino acid metabolism	1741:1770	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	14	8	theme	g_Clostridium	2054:2066	arg1	abundances					2025:2034	the abundances	2021:2034	the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter	2021:2112	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	2	9	theme	sodium-induced	123:136	arg1	colitis					138:144	dextran sulfate sodium-induced colitis	107:144	dextran sulfate sodium-induced colitis in mice	107:152	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	6	10	theme	gut	531:533	arg1	microbiota					535:544	the gut microbiota	527:544	the gut microbiota	527:544	This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.
37351508	9	11	theme	differential	1068:1079	arg1	metabolites					1081:1091	differential metabolites	1068:1091	differential metabolites	1068:1091	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	14	12	from	decrease	2131:2138	arg1	abundance					2147:2155	the abundance	2143:2155	the abundance of g_unclassified_Bacteria induced by DSS	2143:2197	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	9	13	used	used	966:969	arg2	platform					953:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	11	14	theme	activity	1328:1335	arg1	<					1381:1381	p < 0.001)	1379:1388	p < 0.001)	1379:1388	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	14	theme	activity	1328:1335	arg1	score					1343:1347	an increased disease activity index score	1307:1347	an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)]	1307:1389	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	2	15	theme	microbiota	210:219	arg1	modulation					164:173	global modulation	157:173	global modulation of systemic metabolism and the gut microbiota	157:219	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	4	16	dep	obliquus	307:314	arg1	Fr					317:318	Fr	317:318	Fr.	317:319	Gray (HSH) and Inonotus obliquus (Fr.)
37351508	16	17	theme	microbiota	2538:2547	arg1	modulation					2492:2501	global modulation	2485:2501	global modulation of systemic metabolism and the gut microbiota	2485:2547	Discussion: These results suggested that HSH and BHR may ameliorate DSS-induced colitis by global modulation of systemic metabolism and the gut microbiota.
37351508	12	18	theme	pyrimidine	1811:1820	arg1	metabolism					1822:1831	pyrimidine metabolism	1811:1831	pyrimidine metabolism	1811:1831	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	18	theme	pyrimidine	1811:1820	arg1	metabolism					1761:1770	aromatic amino acid metabolism	1741:1770	aromatic amino acid metabolism	1741:1770	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	11	19	dep	index	1549:1553	arg1	±					1561:1561	2.17 ± 0.28 and 1.83 ± 0.29	1556:1582	2.17 ± 0.28 and 1.83 ± 0.29	1556:1582	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	20	theme	disease	1532:1538	arg1	index					1549:1553	the disease activity index	1528:1553	the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively)	1528:1597	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	8	21	theme	Body	724:727	arg1	weight					729:734	Body weight	724:734	Body weight	724:734	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	6	22	theme	metabolism	559:568	arg1	regulation					513:522	the regulation	509:522	the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis	509:606	This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.
37351508	10	23	theme	16S	1117:1119	arg1	sequencing					1131:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing was used to measure the composition of bacterial communities.
37351508	11	24	theme	6.62	1650:1653	arg1	0.35 cm					1657:1663	6.62 ± 0.35 cm	1650:1663	6.62 ± 0.35 cm	1650:1663	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	16	25	theme	DSS-induced	2462:2472	arg1	colitis					2474:2480	DSS-induced colitis	2462:2480	DSS-induced colitis	2462:2480	Discussion: These results suggested that HSH and BHR may ameliorate DSS-induced colitis by global modulation of systemic metabolism and the gut microbiota.
37351508	0	26	from	frondosa	38:45	arg1	polysaccharides					9:23	Mushroom polysaccharides	0:23	Mushroom polysaccharides from Grifola frondosa (Dicks.)	0:54	Mushroom polysaccharides from Grifola frondosa (Dicks.)
37351508	10	27	used	used	1146:1149	arg2	sequencing					1131:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing was used to measure the composition of bacterial communities.
37351508	0	28	theme	Mushroom	0:7	arg1	polysaccharides					9:23	Mushroom polysaccharides	0:23	Mushroom polysaccharides from Grifola frondosa (Dicks.)	0:54	Mushroom polysaccharides from Grifola frondosa (Dicks.)
37351508	11	29	theme	p	1379:1379	arg1	<					1381:1381	p < 0.001)	1379:1388	p < 0.001)	1379:1388	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	29	theme	p	1379:1379	arg1	score					1343:1347	an increased disease activity index score	1307:1347	an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)]	1307:1389	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	30	theme	index	1337:1341	arg1	<					1381:1381	p < 0.001)	1379:1388	p < 0.001)	1379:1388	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	30	theme	index	1337:1341	arg1	score					1343:1347	an increased disease activity index score	1307:1347	an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)]	1307:1389	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	13	31	theme	Shannon	1936:1942	arg1	index					1944:1948	the Shannon index	1932:1948	the Shannon index	1932:1948	HSH and BHR increased the Chao1 index by 64.25% and 60.25%, respectively, and they increased the Shannon index by 13.02% and 10.23%, respectively.
37351508	6	32	theme	DSS-induced	588:598	arg1	colitis					600:606	DSS-induced colitis	588:606	DSS-induced colitis	588:606	This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.
37351508	2	33	theme	global	157:162	arg1	modulation					164:173	global modulation	157:173	global modulation of systemic metabolism and the gut microbiota	157:219	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	11	34	theme	disease	1320:1326	arg1	<					1381:1381	p < 0.001)	1379:1388	p < 0.001)	1379:1388	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	34	theme	disease	1320:1326	arg1	score					1343:1347	an increased disease activity index score	1307:1347	an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)]	1307:1389	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	16	35	theme	systemic	2506:2513	arg1	metabolism					2515:2524	systemic metabolism	2506:2524	systemic metabolism	2506:2524	Discussion: These results suggested that HSH and BHR may ameliorate DSS-induced colitis by global modulation of systemic metabolism and the gut microbiota.
37351508	11	36	theme	±	1424:1424	arg1	0.64 cm					1426:1432	colon length [5.43 ± 0.64 cm	1405:1432	colon length [5.43 ± 0.64 cm	1405:1432	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	7	37	dep	given	637:641	arg1	%					651:651	2.5%	648:651	2.5%	648:651	Methods: C57BL/6J mice were given DSS (2.5%) in water and were treated with HSH and BHR (200 mg/kg/day) by gavage.
37351508	2	38	theme	metabolism	187:196	arg1	modulation					164:173	global modulation	157:173	global modulation of systemic metabolism and the gut microbiota	157:219	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	11	39	theme	length	1411:1416	arg1	0.64 cm					1426:1432	colon length [5.43 ± 0.64 cm	1405:1432	colon length [5.43 ± 0.64 cm	1405:1432	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	14	40	dep	reversed	1996:2003	arg1	both					1991:1994	both	1991:1994	both	1991:1994	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	11	41	theme	colitis	1247:1253	arg1	model					1255:1259	the mouse colitis model	1237:1259	the mouse colitis model	1237:1259	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	42	theme	DSS-induced	1495:1505	arg1	colitis					1507:1513	DSS-induced colitis	1495:1513	DSS-induced colitis	1495:1513	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	43	theme	±	1443:1443	arg1	0.29 cm					1445:1451	7.04 ± 0.29 cm	1438:1451	7.04 ± 0.29 cm (p < 0.001)	1438:1463	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	43	theme	±	1443:1443	arg1	<					1456:1456	p < 0.001	1454:1462	p < 0.001	1454:1462	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	44	theme	6.12	1631:1634	arg1	0.30 cm					1638:1644	6.12 ± 0.30 cm	1631:1644	6.12 ± 0.30 cm	1631:1644	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	5	45	theme	-induced	391:398	arg1	colitis					400:406	dextran sulfate sodium (DSS)-induced colitis	363:406	dextran sulfate sodium (DSS)-induced colitis	363:406	Pilat (BHR) showed noticeable effects on dextran sulfate sodium (DSS)-induced colitis, but their systemic modulation effects have not been fully revealed.
37351508	4	46	theme	Inonotus	298:305	arg1	obliquus					307:314	Inonotus obliquus	298:314	Inonotus obliquus (Fr.)	298:320	Gray (HSH) and Inonotus obliquus (Fr.)
37351508	9	47	theme	gut	1027:1029	arg1	contents					1031:1038	gut contents	1027:1038	gut contents	1027:1038	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	14	48	theme	g_AF12	2069:2074	arg1	abundances					2025:2034	the abundances	2021:2034	the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter	2021:2112	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	14	49	theme	g_unclassified_Bacteria	2160:2182	arg1	abundance					2147:2155	the abundance	2143:2155	the abundance of g_unclassified_Bacteria induced by DSS	2143:2197	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	10	50	theme	gene	1126:1129	arg1	sequencing					1131:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing was used to measure the composition of bacterial communities.
37351508	5	51	theme	modulation	428:437	arg1	effects					439:445	their systemic modulation effects	413:445	their systemic modulation effects	413:445	Pilat (BHR) showed noticeable effects on dextran sulfate sodium (DSS)-induced colitis, but their systemic modulation effects have not been fully revealed.
37351508	12	52	theme	amino	1750:1754	arg1	cycle					1785:1789	the citrate cycle	1773:1789	the citrate cycle	1773:1789	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	52	theme	amino	1750:1754	arg1	metabolism					1799:1808	purine metabolism	1792:1808	purine metabolism	1792:1808	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	52	theme	amino	1750:1754	arg1	metabolism					1822:1831	pyrimidine metabolism	1811:1831	pyrimidine metabolism	1811:1831	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	52	theme	amino	1750:1754	arg1	etc					1834:1836	etc	1834:1836	etc	1834:1836	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	52	theme	amino	1750:1754	arg1	metabolism					1761:1770	aromatic amino acid metabolism	1741:1770	aromatic amino acid metabolism	1741:1770	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	8	53	theme	protective	854:863	arg1	effect					865:870	the protective effect	850:870	the protective effect of the polysaccharides	850:893	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	6	54	theme	microbiota	535:544	arg1	regulation					513:522	the regulation	509:522	the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis	509:606	This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.
37351508	2	55	theme	sulfate	115:121	arg1	colitis					138:144	dextran sulfate sodium-induced colitis	107:144	dextran sulfate sodium-induced colitis in mice	107:152	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	14	56	theme	g_Odoribacter	2039:2051	arg1	abundances					2025:2034	the abundances	2021:2034	the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter	2021:2112	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	3	57	dep	Introduction	222:233	arg1	Polysaccharides					236:250	Polysaccharides	236:250	Introduction: Polysaccharides from Grifola frondosa (Dicks.)	222:281	Introduction: Polysaccharides from Grifola frondosa (Dicks.)
37351508	17	58	theme	systemic	2631:2638	arg1	metabolism					2640:2649	systemic metabolism	2631:2649	systemic metabolism	2631:2649	Targeting the gut microbiota may be a potentially effective strategy to modulate systemic metabolism and treat colitis.
37351508	9	59	theme	metabolic	1097:1105	arg1	pathways					1107:1114	metabolic pathways	1097:1114	metabolic pathways	1097:1114	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	9	60	theme	LC-QTOF/MS-based	913:928	arg1	platform					953:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	6	61	from	HSH	573:575	arg1	colitis					600:606	DSS-induced colitis	588:606	DSS-induced colitis	588:606	This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.
37351508	13	62	theme	Chao1	1865:1869	arg1	index					1871:1875	the Chao1 index	1861:1875	the Chao1 index	1861:1875	HSH and BHR increased the Chao1 index by 64.25% and 60.25%, respectively, and they increased the Shannon index by 13.02% and 10.23%, respectively.
37351508	2	63	theme	gut	206:208	arg1	microbiota					210:219	the gut microbiota	202:219	the gut microbiota	202:219	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	2	64	from	colitis	138:144	arg1	mice					149:152	mice	149:152	mice	149:152	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	6	65	from	BHR	581:583	arg1	colitis					600:606	DSS-induced colitis	588:606	DSS-induced colitis	588:606	This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.
37351508	10	66	theme	communities	1191:1201	arg1	composition					1166:1176	the composition	1162:1176	the composition of bacterial communities	1162:1201	16S rDNA gene sequencing was used to measure the composition of bacterial communities.
37351508	1	67	dep	Gray	56:59	arg1	Fr					84:85	Fr	84:85	Fr.	84:86	Gray and Inonotus obliquus (Fr.)
37351508	1	67	dep	Gray	56:59	arg1	obliquus					74:81	obliquus	74:81	obliquus	74:81	Gray and Inonotus obliquus (Fr.)
37351508	11	68	theme	activity	1540:1547	arg1	index					1549:1553	the disease activity index	1528:1553	the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively)	1528:1597	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	9	69	theme	untargeted	930:939	arg1	platform					953:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	5	70	theme	noticeable	341:350	arg1	effects					352:358	noticeable effects	341:358	noticeable effects on dextran sulfate sodium (DSS)-induced colitis	341:406	Pilat (BHR) showed noticeable effects on dextran sulfate sodium (DSS)-induced colitis, but their systemic modulation effects have not been fully revealed.
37351508	15	71	from	reductions	2231:2240	arg1	g_Ruminococcus					2280:2293	g_Ruminococcus	2280:2293	g_Ruminococcus	2280:2293	Specifically, HSH reversed the reductions in g_unclassified_Lactobacillales and g_Ruminococcus, and BHR reversed the decreases in g_unidentified_Coriobacteriaceae and g_unclassified_Firmicutes.
37351508	15	71	from	reductions	2231:2240	arg1	g_unclassified_Lactobacillales					2245:2274	g_unclassified_Lactobacillales	2245:2274	g_unclassified_Lactobacillales	2245:2274	Specifically, HSH reversed the reductions in g_unclassified_Lactobacillales and g_Ruminococcus, and BHR reversed the decreases in g_unidentified_Coriobacteriaceae and g_unclassified_Firmicutes.
37351508	17	72	theme	effective	2600:2608	arg1	strategy					2610:2617	a potentially effective strategy	2586:2617	a potentially effective strategy to modulate systemic metabolism and treat colitis	2586:2667	Targeting the gut microbiota may be a potentially effective strategy to modulate systemic metabolism and treat colitis.
37351508	8	73	theme	polysaccharides	879:893	arg1	model					840:844	the model	836:844	the model	836:844	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	8	73	theme	polysaccharides	879:893	arg1	effect					865:870	the protective effect	850:870	the protective effect of the polysaccharides	850:893	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	11	74	dep	score	1343:1347	arg1	±					1355:1355	[2.83 ± 0.62 vs. 0.06 ± 0.14	1349:1376	an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)]	1307:1389	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	12	75	theme	purine	1792:1797	arg1	metabolism					1799:1808	purine metabolism	1792:1808	purine metabolism	1792:1808	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	12	75	theme	purine	1792:1797	arg1	metabolism					1761:1770	aromatic amino acid metabolism	1741:1770	aromatic amino acid metabolism	1741:1770	HSH and BHR significantly modulated metabolites involved in aromatic amino acid metabolism, the citrate cycle, purine metabolism, pyrimidine metabolism, etc.
37351508	6	76	theme	systemic	550:557	arg1	metabolism					559:568	systemic metabolism	550:568	systemic metabolism	550:568	This study aimed to investigate the regulation of the gut microbiota and systemic metabolism by HSH and BHR in DSS-induced colitis.
37351508	2	77	theme	dextran	107:113	arg1	sulfate					115:121	dextran sulfate	107:121	dextran sulfate sodium-induced colitis in mice	107:152	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	9	78	theme	colon	1013:1017	arg1	tissue					1019:1024	colon tissue	1013:1024	colon tissue	1013:1024	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	9	79	theme	metabolomic	941:951	arg1	platform					953:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	an LC-QTOF/MS-based untargeted metabolomic platform	910:960	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	8	80	theme	colon	740:744	arg1	length					746:751	colon length	740:751	colon length	740:751	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	11	81	theme	±	1655:1655	arg1	0.35 cm					1657:1663	6.62 ± 0.35 cm	1650:1663	6.62 ± 0.35 cm	1650:1663	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	82	dep	length	1623:1628	arg1	0.30 cm					1638:1644	6.12 ± 0.30 cm	1631:1644	6.12 ± 0.30 cm	1631:1644	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	82	dep	length	1623:1628	arg1	0.35 cm					1657:1663	6.62 ± 0.35 cm	1650:1663	6.62 ± 0.35 cm	1650:1663	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	16	83	theme	global	2485:2490	arg1	modulation					2492:2501	global modulation	2485:2501	global modulation of systemic metabolism and the gut microbiota	2485:2547	Discussion: These results suggested that HSH and BHR may ameliorate DSS-induced colitis by global modulation of systemic metabolism and the gut microbiota.
37351508	10	84	theme	bacterial	1181:1189	arg1	communities					1191:1201	bacterial communities	1181:1201	bacterial communities	1181:1201	16S rDNA gene sequencing was used to measure the composition of bacterial communities.
37351508	14	85	theme	g_Turicibacter	2099:2112	arg1	abundances					2025:2034	the abundances	2021:2034	the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter	2021:2112	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	15	86	from	decreases	2317:2325	arg1	g_unclassified_Firmicutes					2367:2391	g_unclassified_Firmicutes	2367:2391	g_unclassified_Firmicutes	2367:2391	Specifically, HSH reversed the reductions in g_unclassified_Lactobacillales and g_Ruminococcus, and BHR reversed the decreases in g_unidentified_Coriobacteriaceae and g_unclassified_Firmicutes.
37351508	15	86	from	decreases	2317:2325	arg1	g_unidentified_Coriobacteriaceae					2330:2361	g_unidentified_Coriobacteriaceae	2330:2361	g_unidentified_Coriobacteriaceae	2330:2361	Specifically, HSH reversed the reductions in g_unclassified_Lactobacillales and g_Ruminococcus, and BHR reversed the decreases in g_unidentified_Coriobacteriaceae and g_unclassified_Firmicutes.
37351508	11	87	theme	increased	1310:1318	arg1	<					1381:1381	p < 0.001)	1379:1388	p < 0.001)	1379:1388	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	87	theme	increased	1310:1318	arg1	score					1343:1347	an increased disease activity index score	1307:1347	an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)]	1307:1389	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	8	88	theme	H&E	772:774	arg1	staining					787:794	H&E and AB-PAS staining	772:794	staining	787:794	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	16	89	theme	metabolism	2515:2524	arg1	modulation					2492:2501	global modulation	2485:2501	global modulation of systemic metabolism and the gut microbiota	2485:2547	Discussion: These results suggested that HSH and BHR may ameliorate DSS-induced colitis by global modulation of systemic metabolism and the gut microbiota.
37351508	9	90	from	metabolites	987:997	arg1	serum					1006:1010	the serum	1002:1010	the serum	1002:1010	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	9	90	from	metabolites	987:997	arg1	tissue					1019:1024	colon tissue	1013:1024	colon tissue	1013:1024	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	9	90	from	metabolites	987:997	arg1	contents					1031:1038	gut contents	1027:1038	gut contents	1027:1038	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	9	90	from	metabolites	987:997	arg1	faeces					1045:1050	faeces	1045:1050	faeces	1045:1050	Additionally, an LC-QTOF/MS-based untargeted metabolomic platform was used to identify the metabolites in the serum, colon tissue, gut contents, and faeces and investigate differential metabolites and metabolic pathways.
37351508	11	91	theme	[5.43	1418:1422	arg1	0.64 cm					1426:1432	colon length [5.43 ± 0.64 cm	1405:1432	colon length [5.43 ± 0.64 cm	1405:1432	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	8	92	theme	AB-PAS	780:785	arg1	staining					787:794	H&E and AB-PAS staining	772:794	staining	787:794	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	14	93	from	increase	2009:2016	arg1	abundances					2025:2034	the abundances	2021:2034	the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter	2021:2112	They both reversed the increase in the abundances of g_Odoribacter, g_Clostridium, g_AF12, g_Parabacteroides and g_Turicibacter and reversed the decrease in the abundance of g_unclassified_Bacteria induced by DSS.
37351508	17	94	theme	gut	2564:2566	arg1	microbiota					2568:2577	the gut microbiota	2560:2577	the gut microbiota	2560:2577	Targeting the gut microbiota may be a potentially effective strategy to modulate systemic metabolism and treat colitis.
37351508	5	95	from	effects	352:358	arg1	colitis					400:406	dextran sulfate sodium (DSS)-induced colitis	363:406	dextran sulfate sodium (DSS)-induced colitis	363:406	Pilat (BHR) showed noticeable effects on dextran sulfate sodium (DSS)-induced colitis, but their systemic modulation effects have not been fully revealed.
37351508	11	96	theme	colon	1405:1409	arg1	0.64 cm					1426:1432	colon length [5.43 ± 0.64 cm	1405:1432	colon length [5.43 ± 0.64 cm	1405:1432	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	97	theme	colon	1617:1621	arg1	length					1623:1628	the colon length	1613:1628	the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively)	1613:1678	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	10	98	theme	rDNA	1121:1124	arg1	sequencing					1131:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing	1117:1140	16S rDNA gene sequencing was used to measure the composition of bacterial communities.
37351508	2	99	theme	systemic	178:185	arg1	metabolism					187:196	systemic metabolism	178:196	systemic metabolism	178:196	Pilat ameliorated dextran sulfate sodium-induced colitis in mice by global modulation of systemic metabolism and the gut microbiota.
37351508	11	100	theme	7.04	1438:1441	arg1	±					1443:1443	±	1443:1443	±	1443:1443	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	16	101	theme	gut	2534:2536	arg1	microbiota					2538:2547	the gut microbiota	2530:2547	the gut microbiota	2530:2547	Discussion: These results suggested that HSH and BHR may ameliorate DSS-induced colitis by global modulation of systemic metabolism and the gut microbiota.
37351508	8	102	theme	colon	803:807	arg1	staining					787:794	H&E and AB-PAS staining	772:794	staining	787:794	Body weight and colon length were recorded, and H&E and AB-PAS staining of the colon were conducted to evaluate the model and the protective effect of the polysaccharides.
37351508	11	103	theme	p	1454:1454	arg1	0.29 cm					1445:1451	7.04 ± 0.29 cm	1438:1451	7.04 ± 0.29 cm (p < 0.001)	1438:1463	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	103	theme	p	1454:1454	arg1	<					1456:1456	p < 0.001	1454:1462	p < 0.001	1454:1462	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
37351508	11	104	theme	±	1636:1636	arg1	0.30 cm					1638:1644	6.12 ± 0.30 cm	1631:1644	6.12 ± 0.30 cm	1631:1644	Results: The results showed that the mouse colitis model was established successfully, as evidenced by an increased disease activity index score [2.83 ± 0.62 vs. 0.06 ± 0.14 (p < 0.001)] and shortened colon length [5.43 ± 0.64 cm vs. 7.04 ± 0.29 cm (p < 0.001)], and HSH and BHR ameliorated DSS-induced colitis by improving the disease activity index (2.17 ± 0.28 and 1.83 ± 0.29, respectively) and restoring the colon length (6.12 ± 0.30 cm and 6.62 ± 0.35 cm, respectively).
35285714	4	0	theme	surrounding	579:589	arg1	environment					591:601	the surrounding environment	575:601	the surrounding environment	575:601	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	18	1	theme	roles	2889:2893	arg1	understanding					2845:2857	our current understanding	2833:2857	our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems	2833:2967	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	12	2	theme	filtering	2109:2117	arg1	efficiency					2119:2128	high filtering efficiency	2104:2128	high filtering efficiency for particulate organic matter	2104:2159	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	16	3	theme	host	2567:2570	arg1	pressure					2582:2589	host selection pressure	2567:2589	host selection pressure	2567:2589	Such variations were driven mainly by local environments, followed by host selection pressure.
35285714	7	4	dep	Patescibacteria	1161:1175	arg1	Epsilonbacteraeota					1141:1158	Epsilonbacteraeota	1141:1158	Epsilonbacteraeota	1141:1158	Bacteria affiliated with Firmicutes, Spirochaetes, Tenericutes, Bacteroidetes, Epsilonbacteraeota, Patescibacteria, and Fusobacteria were found to be significantly enriched in the clams in comparison to their local environment.
35285714	12	5	theme	population	2079:2088	arg1	densities					2090:2098	its high population densities	2070:2098	its high population densities	2070:2098	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	8	6	theme	core-associated	1318:1332	arg1	OTUs					1344:1347	the core-associated bacterial OTUs	1314:1347	the core-associated bacterial OTUs	1314:1347	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	5	7	theme	bacterial	663:671	arg1	communities					673:683	The associated bacterial communities	648:683	The associated bacterial communities	648:683	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	5	7	theme	bacterial	663:671	arg1	specific					695:702	specific	695:702	specific	695:702	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	16	8	theme	Such	2497:2500	arg1	variations					2502:2511	Such variations	2497:2511	Such variations	2497:2511	Such variations were driven mainly by local environments, followed by host selection pressure.
35285714	1	9	theme	freshwater	190:199	arg1	ecosystems					201:210	freshwater ecosystems	190:210	freshwater ecosystems	190:210	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	8	10	contain	had	1394:1396	arg1	OTUs					1389:1392	the core OTUs	1380:1392	the core OTUs	1380:1392	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	8	10	contain	had	1394:1396	arg1	most					1372:1375	most	1372:1375	most	1372:1375	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	8	10	contain	had	1394:1396	arg2	abundances					1413:1422	lower relative abundances	1398:1422	lower relative abundances	1398:1422	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	8	10	contain	had	1394:1396	arg2	frequencies					1439:1449	occurrence frequencies	1428:1449	occurrence frequencies	1428:1449	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	8	11	theme	OTUs	1344:1347	arg1	analyses					1302:1309	Oligotyping analyses	1290:1309	Oligotyping analyses of the core-associated bacterial OTUs	1290:1347	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	6	12	theme	host	992:995	arg1	pressure					1007:1014	strong host selection pressure	985:1014	strong host selection pressure on bacteria from the surrounding environment	985:1059	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	10	13	dep	taxa	1654:1657	arg1	taxa					1654:1657	the two most abundant taxa	1632:1657	the two most abundant taxa Romboutsia and Paraclostridium	1632:1688	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	10	13	dep	taxa	1654:1657	arg1	Paraclostridium					1674:1688	Paraclostridium	1674:1688	Paraclostridium	1674:1688	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	10	13	dep	taxa	1654:1657	arg1	Romboutsia					1659:1668	Romboutsia	1659:1668	Romboutsia	1659:1668	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	15	14	theme	bacterial	2474:2482	arg1	communities					2484:2494	the associated bacterial communities	2459:2494	the associated bacterial communities	2459:2494	We also observed high intrahabitat variation in the associated bacterial communities.
35285714	5	15	theme	host	839:842	arg1	bacteria					844:851	host bacteria	839:851	host bacteria	839:851	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	8	16	theme	Oligotyping	1290:1300	arg1	analyses					1302:1309	Oligotyping analyses	1290:1309	Oligotyping analyses of the core-associated bacterial OTUs	1290:1347	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	17	17	theme	matter	2779:2784	arg1	digestion					2786:2794	organic matter digestion	2771:2794	organic matter digestion	2771:2794	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	0	18	from	fluminea	76:83	arg1	Taihu					121:125	Taihu	121:125	Taihu	121:125	Intrahabitat Differences in Bacterial Communities Associated with Corbicula fluminea in the Large Shallow Eutrophic Lake Taihu.
35285714	17	19	theme	bacterial	2711:2719	arg1	taxa					2721:2724	bacterial taxa	2711:2724	bacterial taxa with the potential for assisting the host in organic matter digestion	2711:2794	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	10	20	theme	potential	1699:1707	arg1	function					1709:1716	the potential function	1695:1716	the potential function of fermenting polysaccharides for assisting host clams in food digestion	1695:1789	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	9	21	theme	community	1584:1592	arg1	network					1594:1600	the bacterial community network	1570:1600	the bacterial community network	1570:1600	The core bacterial OTUs were found to play an important role in maintaining the stability of the bacterial community network.
35285714	9	22	theme	bacterial	1574:1582	arg1	network					1594:1600	the bacterial community network	1570:1600	the bacterial community network	1570:1600	The core bacterial OTUs were found to play an important role in maintaining the stability of the bacterial community network.
35285714	3	23	theme	surrounding	335:345	arg1	environment					347:357	its surrounding environment	331:357	its surrounding environment	331:357	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	5	24	theme	other	780:784	arg1	sites					786:790	other sites	780:790	other sites	780:790	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	4	25	theme	operational	501:511	arg1	OTUs					530:533	OTUs	530:533	OTUs	530:533	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	25	theme	operational	501:511	arg1	units					523:527	the bacterial operational taxonomic units	487:527	the bacterial operational taxonomic units (OTUs) associated with clams	487:556	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	8	26	theme	lower	1398:1402	arg1	abundances					1413:1422	lower relative abundances	1398:1422	lower relative abundances	1398:1422	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	17	27	with	taxa	2721:2724	arg1	potential					2735:2743	the potential	2731:2743	the potential for assisting the host in organic matter digestion	2731:2794	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	11	28	dep	structured	1906:1915	arg1	both					1917:1920	both	1917:1920	both	1917:1920	Overall, we demonstrate that clam-associated bacteria were spatially dynamic and site specific, which were mainly structured both by local environments and host selection.
35285714	11	28	dep	structured	1906:1915	arg1	selection					1953:1961	host selection	1948:1961	host selection	1948:1961	Overall, we demonstrate that clam-associated bacteria were spatially dynamic and site specific, which were mainly structured both by local environments and host selection.
35285714	12	29	theme	benthic	2025:2031	arg1	clam					2033:2036	an important benthic clam	2012:2036	an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter	2012:2159	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	12	29	theme	benthic	2025:2031	arg1	IMPORTANCE					1964:1973	IMPORTANCE	1964:1973	IMPORTANCE The Asian clam Corbicula fluminea	1964:2007	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	4	30	theme	units	523:527	arg1	two-thirds					473:482	two-thirds	473:482	two-thirds	473:482	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	30	theme	units	523:527	arg1	units					523:527	the bacterial operational taxonomic units	487:527	the bacterial operational taxonomic units (OTUs) associated with clams	487:556	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	30	theme	units	523:527	arg1	OTUs					530:533	OTUs	530:533	OTUs	530:533	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	17	31	theme	bacteria	2659:2666	arg1	source					2629:2634	a source	2627:2634	a source of the clam-associated bacteria	2627:2666	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	17	31	theme	bacteria	2659:2666	arg1	microbes					2608:2615	the local microbes	2598:2615	the local microbes	2598:2615	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	14	32	theme	lake	2396:2399	arg1	bacteria					2401:2408	specific lake bacteria	2387:2408	specific lake bacteria	2387:2408	We found that C. fluminea offers a unique ecological niche for specific lake bacteria.
35285714	1	33	theme	clam	138:141	arg1	fluminea					153:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	1	33	theme	clam	138:141	arg1	zoobenthos					176:185	a keystone zoobenthos	165:185	a keystone zoobenthos in freshwater ecosystems	165:210	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	17	34	theme	host	2669:2672	arg1	selection					2674:2682	host selection	2669:2682	host selection	2669:2682	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	4	35	located	observed	563:570	arg1	environment					591:601	the surrounding environment	575:601	the surrounding environment	575:601	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	35	located	observed	563:570	arg2	two-thirds					473:482	two-thirds	473:482	two-thirds	473:482	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	35	located	observed	563:570	arg2	units					523:527	the bacterial operational taxonomic units	487:527	the bacterial operational taxonomic units (OTUs) associated with clams	487:556	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	35	located	observed	563:570	arg2	OTUs					530:533	OTUs	530:533	OTUs	530:533	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	10	36	theme	host	1762:1765	arg1	clams					1767:1771	host clams	1762:1771	host clams in food digestion	1762:1789	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	5	37	from	influence	826:834	arg1	bacteria					844:851	host bacteria	839:851	host bacteria	839:851	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	7	38	from	clams	1242:1246	arg1	comparison					1251:1260	comparison	1251:1260	comparison to their local environment	1251:1287	Bacteria affiliated with Firmicutes, Spirochaetes, Tenericutes, Bacteroidetes, Epsilonbacteraeota, Patescibacteria, and Fusobacteria were found to be significantly enriched in the clams in comparison to their local environment.
35285714	15	39	from	variation	2446:2454	arg1	communities					2484:2494	the associated bacterial communities	2459:2494	the associated bacterial communities	2459:2494	We also observed high intrahabitat variation in the associated bacterial communities.
35285714	14	40	theme	ecological	2366:2375	arg1	niche					2377:2381	a unique ecological niche	2357:2381	a unique ecological niche for specific lake bacteria	2357:2408	We found that C. fluminea offers a unique ecological niche for specific lake bacteria.
35285714	0	41	theme	Intrahabitat	0:11	arg1	Differences					13:23	Intrahabitat Differences	0:23	Intrahabitat Differences in Bacterial Communities	0:48	Intrahabitat Differences in Bacterial Communities Associated with Corbicula fluminea in the Large Shallow Eutrophic Lake Taihu.
35285714	12	42	theme	The	1975:1977	arg1	fluminea					2000:2007	The Asian clam Corbicula fluminea	1975:2007	IMPORTANCE The Asian clam Corbicula fluminea	1964:2007	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	6	43	theme	bacterial	894:902	arg1	diversities					904:914	bacterial diversities	894:914	bacterial diversities	894:914	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	1	44	theme	Corbicula	143:151	arg1	fluminea					153:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	1	44	theme	Corbicula	143:151	arg1	zoobenthos					176:185	a keystone zoobenthos	165:185	a keystone zoobenthos in freshwater ecosystems	165:210	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	18	45	theme	current	2837:2843	arg1	understanding					2845:2857	our current understanding	2833:2857	our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems	2833:2967	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	3	46	theme	gene	442:445	arg1	sequencing					447:456	rRNA gene sequencing	437:456	rRNA gene sequencing	437:456	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	12	47	theme	clam	1985:1988	arg1	fluminea					2000:2007	The Asian clam Corbicula fluminea	1975:2007	IMPORTANCE The Asian clam Corbicula fluminea	1964:2007	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	10	48	theme	core	1609:1612	arg1	bacteria					1614:1621	These core bacteria	1603:1621	These core bacteria	1603:1621	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	18	49	theme	keynote	2931:2937	arg1	clam					2939:2942	a keynote clam	2929:2942	a keynote clam in freshwater ecosystems	2929:2967	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	5	50	theme	environmental	812:824	arg1	influence					826:834	a local environmental influence	804:834	a local environmental influence on host bacteria	804:851	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	17	51	theme	local	2602:2606	arg1	source					2629:2634	a source	2627:2634	a source of the clam-associated bacteria	2627:2666	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	17	51	theme	local	2602:2606	arg1	microbes					2608:2615	the local microbes	2598:2615	the local microbes	2598:2615	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	11	52	theme	host	1948:1951	arg1	selection					1953:1961	host selection	1948:1961	host selection	1948:1961	Overall, we demonstrate that clam-associated bacteria were spatially dynamic and site specific, which were mainly structured both by local environments and host selection.
35285714	15	53	theme	high	2428:2431	arg1	variation					2446:2454	high intrahabitat variation	2428:2454	high intrahabitat variation in the associated bacterial communities	2428:2494	We also observed high intrahabitat variation in the associated bacterial communities.
35285714	12	54	dep	IMPORTANCE	1964:1973	arg1	fluminea					2000:2007	The Asian clam Corbicula fluminea	1975:2007	IMPORTANCE The Asian clam Corbicula fluminea	1964:2007	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	9	55	theme	core	1481:1484	arg1	OTUs					1496:1499	The core bacterial OTUs	1477:1499	The core bacterial OTUs	1477:1499	The core bacterial OTUs were found to play an important role in maintaining the stability of the bacterial community network.
35285714	0	56	theme	Corbicula	66:74	arg1	fluminea					76:83	Corbicula fluminea	66:83	Corbicula fluminea in the Large Shallow Eutrophic Lake Taihu	66:125	Intrahabitat Differences in Bacterial Communities Associated with Corbicula fluminea in the Large Shallow Eutrophic Lake Taihu.
35285714	4	57	theme	particle-associated	619:637	arg1	samples					639:645	particle-associated samples	619:645	particle-associated samples	619:645	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	12	58	theme	particulate	2134:2144	arg1	matter					2154:2159	particulate organic matter	2134:2159	particulate organic matter	2134:2159	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	16	59	theme	selection	2572:2580	arg1	pressure					2582:2589	host selection pressure	2567:2589	host selection pressure	2567:2589	Such variations were driven mainly by local environments, followed by host selection pressure.
35285714	18	60	theme	ecological	2878:2887	arg1	roles					2889:2893	ecological roles	2878:2893	ecological roles	2878:2893	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	12	61	theme	high	2104:2107	arg1	efficiency					2119:2128	high filtering efficiency	2104:2128	high filtering efficiency for particulate organic matter	2104:2159	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	10	62	theme	abundant	1645:1652	arg1	taxa					1654:1657	the two most abundant taxa	1632:1657	the two most abundant taxa Romboutsia and Paraclostridium	1632:1688	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	10	62	theme	abundant	1645:1652	arg1	Paraclostridium					1674:1688	Paraclostridium	1674:1688	Paraclostridium	1674:1688	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	10	62	theme	abundant	1645:1652	arg1	Romboutsia					1659:1668	Romboutsia	1659:1668	Romboutsia	1659:1668	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	18	63	theme	origins	2866:2872	arg1	understanding					2845:2857	our current understanding	2833:2857	our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems	2833:2967	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	18	64	from	clam	2939:2942	arg1	ecosystems					2958:2967	freshwater ecosystems	2947:2967	freshwater ecosystems	2947:2967	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	1	65	theme	keystone	167:174	arg1	fluminea					153:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	1	65	theme	keystone	167:174	arg1	zoobenthos					176:185	a keystone zoobenthos	165:185	a keystone zoobenthos in freshwater ecosystems	165:210	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	6	66	from	differences	879:889	arg1	compositions					920:931	compositions	920:931	compositions	920:931	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	6	66	from	differences	879:889	arg1	diversities					904:914	bacterial diversities	894:914	bacterial diversities	894:914	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	5	67	theme	associated	652:661	arg1	communities					673:683	The associated bacterial communities	648:683	The associated bacterial communities	648:683	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	5	67	theme	associated	652:661	arg1	specific					695:702	specific	695:702	specific	695:702	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	18	68	theme	microbiota	2902:2911	arg1	origins					2866:2872	origins	2866:2872	origins	2866:2872	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	18	68	theme	microbiota	2902:2911	arg1	roles					2889:2893	ecological roles	2878:2893	ecological roles	2878:2893	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	9	69	theme	important	1523:1531	arg1	role					1533:1536	an important role	1520:1536	an important role	1520:1536	The core bacterial OTUs were found to play an important role in maintaining the stability of the bacterial community network.
35285714	12	70	theme	high	2074:2077	arg1	densities					2090:2098	its high population densities	2070:2098	its high population densities	2070:2098	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	5	71	theme	environmental	724:736	arg1	bacteria					738:745	environmental bacteria	724:745	environmental bacteria from the same site than those at other sites	724:790	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	6	72	theme	selection	997:1005	arg1	pressure					1007:1014	strong host selection pressure	985:1014	strong host selection pressure on bacteria from the surrounding environment	985:1059	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	3	73	theme	bacterial	292:300	arg1	water					383:387	water	383:387	water	383:387	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	3	73	theme	bacterial	292:300	arg1	sediment					370:377	sediment	370:377	sediment	370:377	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	3	73	theme	bacterial	292:300	arg1	communities					302:312	the bacterial communities	288:312	the bacterial communities of this clam	288:325	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	8	74	theme	bacterial	1334:1342	arg1	OTUs					1344:1347	the core-associated bacterial OTUs	1314:1347	the core-associated bacterial OTUs	1314:1347	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	10	75	theme	fermenting	1721:1730	arg1	polysaccharides					1732:1746	fermenting polysaccharides	1721:1746	fermenting polysaccharides	1721:1746	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	6	76	theme	strong	985:990	arg1	pressure					1007:1014	strong host selection pressure	985:1014	strong host selection pressure on bacteria from the surrounding environment	985:1059	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	17	77	theme	organic	2771:2777	arg1	matter					2779:2784	organic matter	2771:2784	organic matter digestion	2771:2794	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	5	78	from	site	761:764	arg1	bacteria					738:745	environmental bacteria	724:745	environmental bacteria from the same site than those at other sites	724:790	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	2	79	theme	associated	226:235	arg1	microbiome					237:246	its associated microbiome	222:246	its associated microbiome	222:246	However, its associated microbiome is not well understood.
35285714	3	80	theme	clam	322:325	arg1	water					383:387	water	383:387	water	383:387	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	3	80	theme	clam	322:325	arg1	environment					347:357	its surrounding environment	331:357	its surrounding environment	331:357	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	3	80	theme	clam	322:325	arg1	sediment					370:377	sediment	370:377	sediment	370:377	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	3	80	theme	clam	322:325	arg1	communities					302:312	the bacterial communities	288:312	the bacterial communities of this clam	288:325	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	17	81	theme	taxa	2721:2724	arg1	enrichments					2696:2706	enrichments	2696:2706	enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion	2696:2794	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	11	82	theme	local	1925:1929	arg1	environments					1931:1942	local environments	1925:1942	local environments	1925:1942	Overall, we demonstrate that clam-associated bacteria were spatially dynamic and site specific, which were mainly structured both by local environments and host selection.
35285714	15	83	theme	associated	2463:2472	arg1	communities					2484:2494	the associated bacterial communities	2459:2494	the associated bacterial communities	2459:2494	We also observed high intrahabitat variation in the associated bacterial communities.
35285714	1	84	theme	Asian	132:136	arg1	fluminea					153:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea	128:160	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	1	84	theme	Asian	132:136	arg1	zoobenthos					176:185	a keystone zoobenthos	165:185	a keystone zoobenthos in freshwater ecosystems	165:210	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	13	85	theme	living	2227:2232	arg1	host					2222:2225	host living	2222:2232	host living	2222:2232	While the associated microbiota is believed to be vital for host living, our knowledge about the compositions, sources, and potential functions is still lacking.
35285714	16	86	dep	driven	2518:2523	arg1	followed					2555:2562	followed	2555:2562	followed by host selection pressure	2555:2589	Such variations were driven mainly by local environments, followed by host selection pressure.
35285714	9	87	theme	network	1594:1600	arg1	stability					1557:1565	the stability	1553:1565	the stability of the bacterial community network	1553:1600	The core bacterial OTUs were found to play an important role in maintaining the stability of the bacterial community network.
35285714	12	88	from	clam	2033:2036	arg1	ecosystems					2052:2061	freshwater ecosystems	2041:2061	freshwater ecosystems	2041:2061	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	4	89	theme	taxonomic	513:521	arg1	OTUs					530:533	OTUs	530:533	OTUs	530:533	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	89	theme	taxonomic	513:521	arg1	units					523:527	the bacterial operational taxonomic units	487:527	the bacterial operational taxonomic units (OTUs) associated with clams	487:556	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	8	90	theme	core	1384:1387	arg1	OTUs					1389:1392	the core OTUs	1380:1392	the core OTUs	1380:1392	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	7	91	theme	local	1271:1275	arg1	environment					1277:1287	their local environment	1265:1287	their local environment	1265:1287	Bacteria affiliated with Firmicutes, Spirochaetes, Tenericutes, Bacteroidetes, Epsilonbacteraeota, Patescibacteria, and Fusobacteria were found to be significantly enriched in the clams in comparison to their local environment.
35285714	1	92	from	zoobenthos	176:185	arg1	ecosystems					201:210	freshwater ecosystems	190:210	freshwater ecosystems	190:210	The Asian clam Corbicula fluminea is a keystone zoobenthos in freshwater ecosystems.
35285714	18	93	dep	origins	2866:2872	arg1	the					2862:2864	the	2862:2864	the	2862:2864	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	6	94	theme	surrounding	1037:1047	arg1	environment					1049:1059	the surrounding environment	1033:1059	the surrounding environment	1033:1059	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	0	95	from	Differences	13:23	arg1	Communities					38:48	Bacterial Communities	28:48	Bacterial Communities	28:48	Intrahabitat Differences in Bacterial Communities Associated with Corbicula fluminea in the Large Shallow Eutrophic Lake Taihu.
35285714	6	96	from	environment	1049:1059	arg1	bacteria					1019:1026	bacteria	1019:1026	bacteria from the surrounding environment	1019:1059	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	17	97	theme	clam-associated	2643:2657	arg1	bacteria					2659:2666	the clam-associated bacteria	2639:2666	the clam-associated bacteria	2639:2666	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	5	98	theme	same	756:759	arg1	site					761:764	the same site	752:764	the same site than those at other sites	752:790	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	11	99	dep	both	1917:1920	arg1	environments					1931:1942	local environments	1925:1942	local environments	1925:1942	Overall, we demonstrate that clam-associated bacteria were spatially dynamic and site specific, which were mainly structured both by local environments and host selection.
35285714	12	100	theme	freshwater	2041:2050	arg1	ecosystems					2052:2061	freshwater ecosystems	2041:2061	freshwater ecosystems	2041:2061	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	8	101	theme	relative	1404:1411	arg1	abundances					1413:1422	lower relative abundances	1398:1422	lower relative abundances	1398:1422	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	14	102	theme	specific	2387:2394	arg1	bacteria					2401:2408	specific lake bacteria	2387:2408	specific lake bacteria	2387:2408	We found that C. fluminea offers a unique ecological niche for specific lake bacteria.
35285714	17	103	from	host	2763:2766	arg1	digestion					2786:2794	organic matter digestion	2771:2794	organic matter digestion	2771:2794	While the local microbes served as a source of the clam-associated bacteria, host selection resulted in enrichments of bacterial taxa with the potential for assisting the host in organic matter digestion.
35285714	4	104	theme	bacterial	491:499	arg1	OTUs					530:533	OTUs	530:533	OTUs	530:533	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	4	104	theme	bacterial	491:499	arg1	units					523:527	the bacterial operational taxonomic units	487:527	the bacterial operational taxonomic units (OTUs) associated with clams	487:556	Approximately two-thirds of the bacterial operational taxonomic units (OTUs) associated with clams were observed in the surrounding environment and mostly from particle-associated samples.
35285714	3	105	theme	rRNA	437:440	arg1	sequencing					447:456	rRNA gene sequencing	437:456	rRNA gene sequencing	437:456	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	12	106	theme	important	2015:2023	arg1	clam					2033:2036	an important benthic clam	2012:2036	an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter	2012:2159	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	12	106	theme	important	2015:2023	arg1	IMPORTANCE					1964:1973	IMPORTANCE	1964:1973	IMPORTANCE The Asian clam Corbicula fluminea	1964:2007	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	14	107	theme	C.	2338:2339	arg1	fluminea					2341:2348	C. fluminea	2338:2348	C. fluminea	2338:2348	We found that C. fluminea offers a unique ecological niche for specific lake bacteria.
35285714	14	108	theme	unique	2359:2364	arg1	niche					2377:2381	a unique ecological niche	2357:2381	a unique ecological niche for specific lake bacteria	2357:2408	We found that C. fluminea offers a unique ecological niche for specific lake bacteria.
35285714	10	109	theme	polysaccharides	1732:1746	arg1	function					1709:1716	the potential function	1695:1716	the potential function of fermenting polysaccharides for assisting host clams in food digestion	1695:1789	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	0	110	theme	Bacterial	28:36	arg1	Communities					38:48	Bacterial Communities	28:48	Bacterial Communities	28:48	Intrahabitat Differences in Bacterial Communities Associated with Corbicula fluminea in the Large Shallow Eutrophic Lake Taihu.
35285714	12	111	theme	Asian	1979:1983	arg1	fluminea					2000:2007	The Asian clam Corbicula fluminea	1975:2007	IMPORTANCE The Asian clam Corbicula fluminea	1964:2007	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	8	112	theme	occurrence	1428:1437	arg1	frequencies					1439:1449	occurrence frequencies	1428:1449	occurrence frequencies	1428:1449	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	8	113	theme	environmental	1454:1466	arg1	samples					1468:1474	environmental samples	1454:1474	environmental samples	1454:1474	Oligotyping analyses of the core-associated bacterial OTUs also demonstrated that most of the core OTUs had lower relative abundances and occurrence frequencies in environmental samples.
35285714	10	114	theme	food	1776:1779	arg1	digestion					1781:1789	food digestion	1776:1789	food digestion	1776:1789	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	12	115	theme	Corbicula	1990:1998	arg1	fluminea					2000:2007	The Asian clam Corbicula fluminea	1975:2007	IMPORTANCE The Asian clam Corbicula fluminea	1964:2007	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	6	116	from	pressure	1007:1014	arg1	bacteria					1019:1026	bacteria	1019:1026	bacteria from the surrounding environment	1019:1059	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	13	117	theme	potential	2286:2294	arg1	functions					2296:2304	potential functions	2286:2304	potential functions	2286:2304	While the associated microbiota is believed to be vital for host living, our knowledge about the compositions, sources, and potential functions is still lacking.
35285714	5	118	theme	local	806:810	arg1	influence					826:834	a local environmental influence	804:834	a local environmental influence on host bacteria	804:851	The associated bacterial communities were site specific and more similar to environmental bacteria from the same site than those at other sites, suggesting a local environmental influence on host bacteria.
35285714	6	119	theme	significant	867:877	arg1	differences					879:889	the significant differences	863:889	the significant differences in bacterial diversities and compositions between the clam and the environment	863:968	However, the significant differences in bacterial diversities and compositions between the clam and the environment also indicated strong host selection pressure on bacteria from the surrounding environment.
35285714	3	120	dep	16S	433:435	arg1	means					424:428	means	424:428	means	424:428	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	13	121	theme	associated	2172:2181	arg1	vital					2212:2216	vital	2212:2216	vital	2212:2216	While the associated microbiota is believed to be vital for host living, our knowledge about the compositions, sources, and potential functions is still lacking.
35285714	13	121	theme	associated	2172:2181	arg1	microbiota					2183:2192	the associated microbiota	2168:2192	the associated microbiota	2168:2192	While the associated microbiota is believed to be vital for host living, our knowledge about the compositions, sources, and potential functions is still lacking.
35285714	3	122	theme	large	410:414	arg1	lake					416:419	a large lake	408:419	a large lake	408:419	We investigated the bacterial communities of this clam and its surrounding environment, including sediment and water simultaneously, in a large lake by means of 16S rRNA gene sequencing.
35285714	11	123	theme	clam-associated	1821:1835	arg1	bacteria					1837:1844	clam-associated bacteria	1821:1844	clam-associated bacteria	1821:1844	Overall, we demonstrate that clam-associated bacteria were spatially dynamic and site specific, which were mainly structured both by local environments and host selection.
35285714	16	124	theme	local	2535:2539	arg1	environments					2541:2552	local environments	2535:2552	local environments	2535:2552	Such variations were driven mainly by local environments, followed by host selection pressure.
35285714	15	125	theme	intrahabitat	2433:2444	arg1	variation					2446:2454	high intrahabitat variation	2428:2454	high intrahabitat variation in the associated bacterial communities	2428:2494	We also observed high intrahabitat variation in the associated bacterial communities.
35285714	12	126	theme	organic	2146:2152	arg1	matter					2154:2159	particulate organic matter	2134:2159	particulate organic matter	2134:2159	IMPORTANCE The Asian clam Corbicula fluminea is an important benthic clam in freshwater ecosystems due to its high population densities and high filtering efficiency for particulate organic matter.
35285714	10	127	from	clams	1767:1771	arg1	digestion					1781:1789	food digestion	1776:1789	food digestion	1776:1789	These core bacteria included the two most abundant taxa Romboutsia and Paraclostridium with the potential function of fermenting polysaccharides for assisting host clams in food digestion.
35285714	18	128	theme	freshwater	2947:2956	arg1	ecosystems					2958:2967	freshwater ecosystems	2947:2967	freshwater ecosystems	2947:2967	These results significantly advance our current understanding of the origins and ecological roles of the microbiota associated with a keynote clam in freshwater ecosystems.
35285714	9	129	theme	bacterial	1486:1494	arg1	OTUs					1496:1499	The core bacterial OTUs	1477:1499	The core bacterial OTUs	1477:1499	The core bacterial OTUs were found to play an important role in maintaining the stability of the bacterial community network.
36095950	5	0	dep	factors	841:847	arg1	aggrecan					865:872	aggrecan	865:872	aggrecan	865:872	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	0	dep	factors	841:847	arg1	factors					841:847	the anabolism factors collagen II and aggrecan	827:872	the anabolism factors collagen II and aggrecan	827:872	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	0	dep	factors	841:847	arg1	collagen					849:856	collagen II	849:859	collagen II	849:859	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	1	1	theme	cartilage	182:190	arg1	degeneration					192:203	cartilage degeneration	182:203	cartilage degeneration	182:203	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	3	2	from	drug	557:560	arg1	future					576:581	future	576:581	future	576:581	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	5	3	theme	low	783:785	arg1	concentrations					791:804	low SAL concentrations	783:804	low SAL concentrations	783:804	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	4	with	metalloproteinase-13	923:942	arg1	ADAMTS5					1004:1010	ADAMTS5	1004:1010	ADAMTS5	1004:1010	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	4	with	metalloproteinase-13	923:942	arg1	motifs-5					994:1001	thrombospondin motifs-5	979:1001	thrombospondin motifs-5 (ADAMTS5)	979:1011	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	5	theme	matrix	916:921	arg1	MMP13					945:949	MMP13	945:949	MMP13	945:949	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	5	theme	matrix	916:921	arg1	metalloproteinase-13					923:942	the catabolic factors matrix metalloproteinase-13	894:942	the catabolic factors matrix metalloproteinase-13 (MMP13)	894:950	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	6	6	theme	surgery-induced	1329:1343	arg1	model					1354:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	6	7	theme	DMM	1324:1326	arg1	model					1354:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	3	8	used	used	479:482	arg2	drug					501:504	an anti-tumor drug	487:504	an anti-tumor drug	487:504	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	8	used	used	479:482	arg2	ionophore					461:469	a natural carboxylic polyether ionophore	430:469	a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL),	430:524	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	8	used	used	479:482	arg2	drug					557:560	a promising therapeutic drug	533:560	a promising therapeutic drug for OA	533:567	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	2	9	theme	available	334:342	arg1	treatments					323:332	no disease-modifying treatments	302:332	no disease-modifying treatments available for OA	302:349	Currently there are no disease-modifying treatments available for OA because its etiology and pathogenesis are largely unknown.
36095950	6	10	theme	meniscus	1314:1321	arg1	model					1354:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	7	11	theme	Lrp6	1476:1479	arg1	phosphorylation					1481:1495	Lrp6 phosphorylation	1476:1495	Lrp6 phosphorylation	1476:1495	Mechanistically, a low SAL concentration induced anabolism and inhibited catabolism in chondrocytes via inhibiting Lrp6 phosphorylation and Wnt/β-catenin signaling.
36095950	3	12	theme	therapeutic	545:555	arg1	ionophore					461:469	a natural carboxylic polyether ionophore	430:469	a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL),	430:524	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	12	theme	therapeutic	545:555	arg1	drug					557:560	a promising therapeutic drug	533:560	a promising therapeutic drug for OA	533:567	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	7	13	theme	SAL	1384:1386	arg1	concentration					1388:1400	a low SAL concentration	1378:1400	a low SAL concentration	1378:1400	Mechanistically, a low SAL concentration induced anabolism and inhibited catabolism in chondrocytes via inhibiting Lrp6 phosphorylation and Wnt/β-catenin signaling.
36095950	4	14	theme	protective	663:672	arg1	effect					674:679	a protective effect	661:679	a protective effect	661:679	We found that SAL showed no cytotoxicity on mouse chondrocytes and displayed a protective effect against interleukin-1β (IL-1β), in cultured mouse chondrocytes and cartilage explants.
36095950	0	15	theme	Wnt/β-catenin	65:77	arg1	signaling					79:87	Wnt/β-catenin signaling	65:87	Wnt/β-catenin signaling	65:87	Salinomycin alleviates osteoarthritis progression via inhibiting Wnt/β-catenin signaling.
36095950	5	16	from	responses	1105:1113	arg1	chondrocytes					1124:1135	mouse chondrocytes	1118:1135	mouse chondrocytes	1118:1135	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	2	17	theme	disease-modifying	305:321	arg1	treatments					323:332	no disease-modifying treatments	302:332	no disease-modifying treatments available for OA	302:349	Currently there are no disease-modifying treatments available for OA because its etiology and pathogenesis are largely unknown.
36095950	1	18	theme	synovial	206:213	arg1	hyperplasia					215:225	synovial hyperplasia	206:225	synovial hyperplasia	206:225	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	8	19	theme	OA	1620:1621	arg1	pathogenesis					1623:1634	OA pathogenesis	1620:1634	OA pathogenesis	1620:1634	Our results suggested that SAL may serve as a potential disease-modifying therapeutic against OA pathogenesis.
36095950	6	20	theme	destabilized	1294:1305	arg1	model					1354:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	3	21	theme	natural	432:438	arg1	drug					501:504	an anti-tumor drug	487:504	an anti-tumor drug	487:504	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	21	theme	natural	432:438	arg1	ionophore					461:469	a natural carboxylic polyether ionophore	430:469	a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL),	430:524	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	21	theme	natural	432:438	arg1	drug					557:560	a promising therapeutic drug	533:560	a promising therapeutic drug for OA	533:567	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	6	22	theme	bone	1248:1251	arg1	sclerosis					1253:1261	subchondral bone sclerosis	1236:1261	subchondral bone sclerosis	1236:1261	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	6	23	theme	changes	1193:1199	arg1	severity					1167:1174	the severity	1163:1174	the severity of OA-associated changes	1163:1199	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	5	24	with	metalloproteinase	956:972	arg1	ADAMTS5					1004:1010	ADAMTS5	1004:1010	ADAMTS5	1004:1010	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	24	with	metalloproteinase	956:972	arg1	motifs-5					994:1001	thrombospondin motifs-5	979:1001	thrombospondin motifs-5 (ADAMTS5)	979:1011	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	25	theme	anabolism	831:839	arg1	aggrecan					865:872	aggrecan	865:872	aggrecan	865:872	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	25	theme	anabolism	831:839	arg1	factors					841:847	the anabolism factors collagen II and aggrecan	827:872	the anabolism factors collagen II and aggrecan	827:872	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	25	theme	anabolism	831:839	arg1	collagen					849:856	collagen II	849:859	collagen II	849:859	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	26	theme	thrombospondin	979:992	arg1	ADAMTS5					1004:1010	ADAMTS5	1004:1010	ADAMTS5	1004:1010	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	26	theme	thrombospondin	979:992	arg1	motifs-5					994:1001	thrombospondin motifs-5	979:1001	thrombospondin motifs-5 (ADAMTS5)	979:1011	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	1	27	theme	osteophyte	228:237	arg1	formation					239:247	osteophyte formation	228:247	osteophyte formation	228:247	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	0	28	theme	osteoarthritis	23:36	arg1	progression					38:48	osteoarthritis progression	23:48	osteoarthritis progression	23:48	Salinomycin alleviates osteoarthritis progression via inhibiting Wnt/β-catenin signaling.
36095950	6	29	theme	cartilage	1213:1221	arg1	destruction					1223:1233	cartilage destruction	1213:1233	cartilage destruction	1213:1233	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	5	30	theme	inflammatory	1092:1103	arg1	responses					1105:1113	inflammatory responses	1092:1113	inflammatory responses in mouse chondrocytes	1092:1135	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	4	31	theme	cartilage	748:756	arg1	explants					758:765	cartilage explants	748:765	cartilage explants	748:765	We found that SAL showed no cytotoxicity on mouse chondrocytes and displayed a protective effect against interleukin-1β (IL-1β), in cultured mouse chondrocytes and cartilage explants.
36095950	7	32	from	catabolism	1434:1443	arg1	chondrocytes					1448:1459	chondrocytes	1448:1459	chondrocytes	1448:1459	Mechanistically, a low SAL concentration induced anabolism and inhibited catabolism in chondrocytes via inhibiting Lrp6 phosphorylation and Wnt/β-catenin signaling.
36095950	4	33	theme	mouse	628:632	arg1	chondrocytes					634:645	mouse chondrocytes	628:645	mouse chondrocytes	628:645	We found that SAL showed no cytotoxicity on mouse chondrocytes and displayed a protective effect against interleukin-1β (IL-1β), in cultured mouse chondrocytes and cartilage explants.
36095950	5	34	theme	SAL	787:789	arg1	concentrations					791:804	low SAL concentrations	783:804	low SAL concentrations	783:804	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	6	35	theme	subchondral	1236:1246	arg1	sclerosis					1253:1261	subchondral bone sclerosis	1236:1261	subchondral bone sclerosis	1236:1261	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	3	36	theme	anti-tumor	490:499	arg1	drug					501:504	an anti-tumor drug	487:504	an anti-tumor drug	487:504	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	36	theme	anti-tumor	490:499	arg1	ionophore					461:469	a natural carboxylic polyether ionophore	430:469	a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL),	430:524	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	36	theme	anti-tumor	490:499	arg1	salinomycin					507:517	salinomycin	507:517	salinomycin (SAL)	507:523	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	1	37	theme	bone	266:269	arg1	sclerosis					271:279	subchondral bone sclerosis	254:279	subchondral bone sclerosis	254:279	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	3	38	theme	carboxylic	440:449	arg1	drug					501:504	an anti-tumor drug	487:504	an anti-tumor drug	487:504	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	38	theme	carboxylic	440:449	arg1	ionophore					461:469	a natural carboxylic polyether ionophore	430:469	a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL),	430:524	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	38	theme	carboxylic	440:449	arg1	drug					557:560	a promising therapeutic drug	533:560	a promising therapeutic drug for OA	533:567	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	5	39	theme	catabolic	898:906	arg1	factors					908:914	catabolic factors	898:914	the catabolic factors matrix metalloproteinase-13 (MMP13)	894:950	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	40	theme	mouse	1118:1122	arg1	chondrocytes					1124:1135	mouse chondrocytes	1118:1135	mouse chondrocytes	1118:1135	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	6	41	theme	osteophyte	1268:1277	arg1	formation					1279:1287	osteophyte formation	1268:1287	osteophyte formation	1268:1287	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	1	42	theme	prevalent	122:130	arg1	disease					157:163	the most prevalent degenerative whole-joint disease	113:163	the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis	113:279	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	1	42	theme	prevalent	122:130	arg1	Osteoarthritis					90:103	Osteoarthritis	90:103	Osteoarthritis (OA)	90:108	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	3	43	theme	polyether	451:459	arg1	drug					501:504	an anti-tumor drug	487:504	an anti-tumor drug	487:504	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	43	theme	polyether	451:459	arg1	ionophore					461:469	a natural carboxylic polyether ionophore	430:469	a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL),	430:524	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	43	theme	polyether	451:459	arg1	drug					557:560	a promising therapeutic drug	533:560	a promising therapeutic drug for OA	533:567	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	6	44	theme	medial	1307:1312	arg1	model					1354:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	1	45	theme	degenerative	132:143	arg1	disease					157:163	the most prevalent degenerative whole-joint disease	113:163	the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis	113:279	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	1	45	theme	degenerative	132:143	arg1	Osteoarthritis					90:103	Osteoarthritis	90:103	Osteoarthritis (OA)	90:108	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	5	46	with	Treatment	768:776	arg1	concentrations					791:804	low SAL concentrations	783:804	low SAL concentrations	783:804	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	6	47	theme	OA	1351:1352	arg1	model					1354:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	1	48	theme	whole-joint	145:155	arg1	disease					157:163	the most prevalent degenerative whole-joint disease	113:163	the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis	113:279	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	1	48	theme	whole-joint	145:155	arg1	Osteoarthritis					90:103	Osteoarthritis	90:103	Osteoarthritis (OA)	90:108	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	6	49	theme	OA-associated	1179:1191	arg1	changes					1193:1199	OA-associated changes	1179:1199	OA-associated changes	1179:1199	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	1	50	theme	subchondral	254:264	arg1	bone					266:269	subchondral bone	254:269	subchondral bone sclerosis	254:279	Osteoarthritis (OA) is the most prevalent degenerative whole-joint disease characterized by cartilage degeneration, synovial hyperplasia, osteophyte formation, and subchondral bone sclerosis.
36095950	6	51	theme	mouse	1345:1349	arg1	model					1354:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	a destabilized medial meniscus (DMM) surgery-induced mouse OA model	1292:1358	Furthermore, SAL reduced the severity of OA-associated changes and delayed cartilage destruction, subchondral bone sclerosis, and osteophyte formation in a destabilized medial meniscus (DMM) surgery-induced mouse OA model.
36095950	7	52	theme	Wnt/β-catenin	1501:1513	arg1	signaling					1515:1523	Wnt/β-catenin signaling	1501:1523	Wnt/β-catenin signaling	1501:1523	Mechanistically, a low SAL concentration induced anabolism and inhibited catabolism in chondrocytes via inhibiting Lrp6 phosphorylation and Wnt/β-catenin signaling.
36095950	4	53	theme	cultured	716:723	arg1	chondrocytes					731:742	cultured mouse chondrocytes	716:742	cultured mouse chondrocytes	716:742	We found that SAL showed no cytotoxicity on mouse chondrocytes and displayed a protective effect against interleukin-1β (IL-1β), in cultured mouse chondrocytes and cartilage explants.
36095950	4	54	theme	mouse	725:729	arg1	chondrocytes					731:742	cultured mouse chondrocytes	716:742	cultured mouse chondrocytes	716:742	We found that SAL showed no cytotoxicity on mouse chondrocytes and displayed a protective effect against interleukin-1β (IL-1β), in cultured mouse chondrocytes and cartilage explants.
36095950	5	55	theme	extracellular	1032:1044	arg1	ECM					1054:1056	ECM	1054:1056	ECM	1054:1056	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	55	theme	extracellular	1032:1044	arg1	matrix					1046:1051	extracellular matrix	1032:1051	extracellular matrix (ECM) degradation	1032:1069	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	56	theme	factors	908:914	arg1	MMP13					945:949	MMP13	945:949	MMP13	945:949	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	5	56	theme	factors	908:914	arg1	metalloproteinase-13					923:942	the catabolic factors matrix metalloproteinase-13	894:942	the catabolic factors matrix metalloproteinase-13 (MMP13)	894:950	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	7	57	theme	low	1380:1382	arg1	concentration					1388:1400	a low SAL concentration	1378:1400	a low SAL concentration	1378:1400	Mechanistically, a low SAL concentration induced anabolism and inhibited catabolism in chondrocytes via inhibiting Lrp6 phosphorylation and Wnt/β-catenin signaling.
36095950	5	58	theme	matrix	1046:1051	arg1	degradation					1059:1069	extracellular matrix (ECM) degradation	1032:1069	extracellular matrix (ECM) degradation	1032:1069	Treatment with low SAL concentrations directly upregulated the anabolism factors collagen II and aggrecan, while it inhibited the catabolic factors matrix metalloproteinase-13 (MMP13) and metalloproteinase with thrombospondin motifs-5 (ADAMTS5) to protect against extracellular matrix (ECM) degradation, and also suppressed inflammatory responses in mouse chondrocytes.
36095950	3	59	theme	promising	535:543	arg1	ionophore					461:469	a natural carboxylic polyether ionophore	430:469	a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL),	430:524	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
36095950	3	59	theme	promising	535:543	arg1	drug					557:560	a promising therapeutic drug	533:560	a promising therapeutic drug for OA	533:567	Here we report that a natural carboxylic polyether ionophore that is used as an anti-tumor drug, salinomycin (SAL), may be a promising therapeutic drug for OA in the future.
37147551	6	0	theme	net	1016:1018	arg1	carbon					1020:1025	net carbon	1016:1025	net carbon	1016:1025	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	9	1	theme	communities	1766:1776	arg1	networks					1744:1751	the networks	1740:1751	the networks of bacterial communities in BSCs	1740:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	9	1	theme	communities	1766:1776	arg1	components					1718:1727	the monosaccharide components	1699:1727	the monosaccharide components of EPS	1699:1734	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	5	2	from	taxa	914:917	arg1	stages					932:937	the later stages	922:937	the later stages	922:937	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	6	3	theme	respiration	1097:1107	arg1	rate					1109:1112	a significantly higher respiration rate	1074:1112	a significantly higher respiration rate	1074:1112	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	0	4	theme	exchange	76:83	arg1	Changes					0:6	Changes	0:6	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.	0:140	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	1	5	theme	global	256:261	arg1	flux					278:281	global biogeochemical flux	256:281	global biogeochemical flux	256:281	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	0	6	theme	successional	95:106	arg1	stages					108:113	the successional stages	91:113	the successional stages of biological soil crusts	91:139	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	7	7	theme	successional	1209:1220	arg1	stages					1222:1227	BSCs' successional stages	1203:1227	BSCs' successional stages	1203:1227	The monosaccharide analysis indicated that the EPSs components also varied depending on BSCs' successional stages.
37147551	9	8	theme	EPS	1732:1734	arg1	networks					1744:1751	the networks	1740:1751	the networks of bacterial communities in BSCs	1740:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	9	8	theme	EPS	1732:1734	arg1	components					1718:1727	the monosaccharide components	1699:1727	the monosaccharide components of EPS	1699:1734	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	3	9	theme	successional	586:597	arg1	stages					599:604	different successional stages	576:604	different successional stages	576:604	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	9	10	theme	monosaccharide	1703:1716	arg1	components					1718:1727	the monosaccharide components	1699:1727	the monosaccharide components of EPS	1699:1734	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	1	11	theme	biogeochemical	263:276	arg1	flux					278:281	global biogeochemical flux	256:281	global biogeochemical flux	256:281	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	8	12	theme	rhamnose	1260:1267	arg1	contents					1248:1255	the contents	1244:1255	the contents of rhamnose and arabinose	1244:1281	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	8	12	theme	rhamnose	1260:1267	arg1	higher					1288:1293	higher	1288:1293	higher	1288:1293	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	5	13	theme	taxa in	795:801	arg1	Cyanobacteria					767:779	Cyanobacteria	767:779	Cyanobacteria	767:779	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	5	13	theme	taxa in	795:801	arg1	stage					813:817	the early stage	803:817	keystone taxa in the early stage	786:817	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	8	14	theme	cyanobacterial-lichen	1430:1450	arg1	crusts					1452:1457	cyanobacterial-lichen crusts	1430:1457	cyanobacterial-lichen crusts	1430:1457	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	1	15	theme	Biological	142:151	arg1	crusts					158:163	Biological soil crusts	142:163	Biological soil crusts (BSCs)	142:170	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	1	15	theme	Biological	142:151	arg1	landscape					198:206	a dominant ecological landscape	176:206	a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux	176:281	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	1	15	theme	Biological	142:151	arg1	BSCs					166:169	BSCs	166:169	BSCs	166:169	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	0	16	theme	of biological	115:127	arg1	crusts					134:139	the successional stages of biological soil crusts	91:139	the successional stages of biological soil crusts	91:139	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	9	17	theme	and this	1604:1611	arg1	work					1613:1616	and this work	1604:1616	and this work	1604:1616	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	5	18	theme	later	926:930	arg1	stages					932:937	the later stages	922:937	the later stages	922:937	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	4	19	theme	dominant	650:657	arg1	species					669:675	dominant bacterial species	650:675	dominant bacterial species	650:675	Our findings demonstrated that besides the dominant bacterial species, the bacterial communities also showed considerable differences between these two stages.
37147551	8	20	theme	and galactose	1460:1472	arg1	highest					1419:1425	highest	1419:1425	highest	1419:1425	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	8	20	theme	and galactose	1460:1472	arg1	content was					1474:1484	and galactose content was	1460:1484	and galactose content was	1460:1484	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	8	21	theme	the cyanobacterial	1298:1315	arg1	types					1335:1339	the cyanobacterial crusts than other types	1298:1339	the cyanobacterial crusts than other types of crusts	1298:1349	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	3	22	theme	physiological	454:466	arg1	characteristics					468:482	physiological characteristics	454:482	physiological characteristics	454:482	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	0	23	theme	stages	108:113	arg1	crusts					134:139	the successional stages of biological soil crusts	91:139	the successional stages of biological soil crusts	91:139	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	9	24	theme	fresh	1628:1632	arg1	viewpoint					1634:1642	a fresh viewpoint	1626:1642	a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs	1626:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	3	25	theme	different	576:584	arg1	stages					599:604	different successional stages	576:604	different successional stages	576:604	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	6	26	theme	of CO2	965:970	arg1	exchange					972:979	of CO2 exchange	965:979	of CO2 exchange	965:979	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	2	27	theme	bacterial	311:319	arg1	characteristics					349:363	bacterial community and physiological characteristics	311:363	bacterial community and physiological characteristics	311:363	However, it is unclear how bacterial community and physiological characteristics vary along the BSCs successional stages.
37147551	9	28	from	networks	1744:1751	arg1	BSCs					1781:1784	BSCs	1781:1784	BSCs	1781:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	5	29	theme	keystone	905:912	arg1	taxa					914:917	keystone taxa	905:917	keystone taxa	905:917	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	1	30	theme	dominant ecological	178:196	arg1	crusts					158:163	Biological soil crusts	142:163	Biological soil crusts (BSCs)	142:170	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	1	30	theme	dominant ecological	178:196	arg1	landscape					198:206	a dominant ecological landscape	176:206	a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux	176:281	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	0	31	theme	community	21:29	arg1	structure					31:39	bacterial community structure	11:39	bacterial community structure	11:39	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	8	32	theme	arabinose	1273:1281	arg1	contents					1248:1255	the contents	1244:1255	the contents of rhamnose and arabinose	1244:1281	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	8	32	theme	arabinose	1273:1281	arg1	higher					1288:1293	higher	1288:1293	higher	1288:1293	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	5	33	theme	keystone	786:793	arg1	Cyanobacteria					767:779	Cyanobacteria	767:779	Cyanobacteria	767:779	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	5	33	theme	keystone	786:793	arg1	stage					813:817	the early stage	803:817	keystone taxa in the early stage	786:817	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	8	34	theme	crusts than	1317:1327	arg1	types					1335:1339	the cyanobacterial crusts than other types	1298:1339	the cyanobacterial crusts than other types of crusts	1298:1349	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	3	35	dep	 were	555:559	arg1	compared					561:568	compared	561:568	 were compared among different successional stages	555:604	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	9	36	theme	heterogeneous	1549:1561	arg1	variation					1563:1571	the heterogeneous variation	1545:1571	the heterogeneous variation of BSCs	1545:1579	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	3	37	theme	monosaccharide	489:502	arg1	composition					504:514	monosaccharide composition	489:514	monosaccharide composition	489:514	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	0	38	theme	bacterial	11:19	arg1	structure					31:39	bacterial community structure	11:39	bacterial community structure	11:39	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	6	39	theme	while moss	1052:1061	arg1	crusts					1063:1068	while moss crusts	1052:1068	while moss crusts	1052:1068	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	0	40	theme	soil	129:132	arg1	crusts					134:139	the successional stages of biological soil crusts	91:139	the successional stages of biological soil crusts	91:139	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	8	41	theme	in the	1494:1499	arg1	crusts					1506:1511	highest in the moss crusts	1486:1511	highest in the moss crusts	1486:1511	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	8	42	theme	highest	1486:1492	arg1	crusts					1506:1511	highest in the moss crusts	1486:1511	highest in the moss crusts	1486:1511	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	2	43	theme	BSCs successional	380:396	arg1	stages					398:403	the BSCs successional stages	376:403	the BSCs successional stages	376:403	However, it is unclear how bacterial community and physiological characteristics vary along the BSCs successional stages.
37147551	3	44	theme	bacterial	421:429	arg1	composition					441:451	bacterial community composition	421:451	bacterial community composition	421:451	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	9	45	theme	interactions	1678:1689	arg1	comprehension					1657:1669	a deeper comprehension	1648:1669	a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs	1648:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	5	46	dep	bacteria	840:847	arg1	Proteobacteria					850:863	Proteobacteria	850:863	Proteobacteria	850:863	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	4	47	theme	considerable	716:727	arg1	differences					729:739	considerable differences	716:739	considerable differences between these two stages	716:764	Our findings demonstrated that besides the dominant bacterial species, the bacterial communities also showed considerable differences between these two stages.
37147551	5	48	theme	early	807:811	arg1	Cyanobacteria					767:779	Cyanobacteria	767:779	Cyanobacteria	767:779	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	5	48	theme	early	807:811	arg1	stage					813:817	the early stage	803:817	keystone taxa in the early stage	786:817	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	9	49	theme	BSCs	1576:1579	arg1	variation					1563:1571	the heterogeneous variation	1545:1571	the heterogeneous variation of BSCs	1545:1579	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	1	50	theme	drylands	211:218	arg1	crusts					158:163	Biological soil crusts	142:163	Biological soil crusts (BSCs)	142:170	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	1	50	theme	drylands	211:218	arg1	landscape					198:206	a dominant ecological landscape	176:206	a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux	176:281	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	0	51	theme	structure	31:39	arg1	Changes					0:6	Changes	0:6	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.	0:140	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	6	52	theme	cyanobacterial	982:995	arg1	crusts					997:1002	cyanobacterial crusts	982:1002	cyanobacterial crusts	982:1002	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	3	53	theme	community	431:439	arg1	composition					441:451	bacterial community composition	421:451	bacterial community composition	421:451	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	6	54	theme	higher	1090:1095	arg1	rate					1109:1112	a significantly higher respiration rate	1074:1112	a significantly higher respiration rate	1074:1112	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	4	55	theme	bacterial	682:690	arg1	communities					692:702	the bacterial communities	678:702	the bacterial communities	678:702	Our findings demonstrated that besides the dominant bacterial species, the bacterial communities also showed considerable differences between these two stages.
37147551	5	56	theme	heterotrophic	826:838	arg1	bacteria					840:847	heterotrophic bacteria	826:847	heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria)	826:898	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	0	57	theme	composition	56:66	arg1	Changes					0:6	Changes	0:6	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.	0:140	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	6	58	theme	moss	1039:1042	arg1	crusts					1044:1049	moss crusts	1039:1049	moss crusts	1039:1049	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	8	59	theme	other	1329:1333	arg1	types					1335:1339	the cyanobacterial crusts than other types	1298:1339	the cyanobacterial crusts than other types of crusts	1298:1349	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	1	60	contain	have	227:230	arg2	impact					246:251	a significant impact	232:251	a significant impact	232:251	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	1	60	contain	have	227:230	arg1	drylands					211:218	drylands	211:218	drylands	211:218	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	7	61	theme	monosaccharide	1119:1132	arg1	analysis					1134:1141	The monosaccharide analysis	1115:1141	The monosaccharide analysis	1115:1141	The monosaccharide analysis indicated that the EPSs components also varied depending on BSCs' successional stages.
37147551	3	62	theme	polysaccharides	533:547	arg1	composition					441:451	bacterial community composition	421:451	bacterial community composition	421:451	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	3	62	theme	polysaccharides	533:547	arg1	characteristics					468:482	physiological characteristics	454:482	physiological characteristics	454:482	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	3	62	theme	polysaccharides	533:547	arg1	composition					504:514	monosaccharide composition	489:514	monosaccharide composition	489:514	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	0	63	theme	monosaccharide	41:54	arg1	composition					56:66	monosaccharide composition	41:66	monosaccharide composition	41:66	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	9	64	from	communities	1766:1776	arg1	BSCs					1781:1784	BSCs	1781:1784	BSCs	1781:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	5	65	dep	Proteobacteria	850:863	arg1	Acidobacteria					885:897	Acidobacteria	885:897	Acidobacteria	885:897	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	5	65	dep	Proteobacteria	850:863	arg1	Actinobacteria					866:879	Actinobacteria	866:879	Actinobacteria	866:879	Cyanobacteria were keystone taxa in the early stage, while heterotrophic bacteria (Proteobacteria, Actinobacteria and Acidobacteria) were keystone taxa in the later stages.
37147551	6	66	contain	had	1070:1072	arg1	crusts					1063:1068	while moss crusts	1052:1068	while moss crusts	1052:1068	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	6	66	contain	had	1070:1072	arg2	rate					1109:1112	a significantly higher respiration rate	1074:1112	a significantly higher respiration rate	1074:1112	According to the results of CO2 exchange, cyanobacterial crusts accumulated net carbon faster than moss crusts, while moss crusts had a significantly higher respiration rate.
37147551	2	67	theme	physiological	335:347	arg1	characteristics					349:363	bacterial community and physiological characteristics	311:363	bacterial community and physiological characteristics	311:363	However, it is unclear how bacterial community and physiological characteristics vary along the BSCs successional stages.
37147551	9	68	theme	bacterial	1756:1764	arg1	communities					1766:1776	bacterial communities	1756:1776	bacterial communities in BSCs	1756:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	8	69	dep	highest	1419:1425	arg1	crusts					1506:1511	highest in the moss crusts	1486:1511	highest in the moss crusts	1486:1511	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	8	70	theme	moss	1501:1504	arg1	crusts					1506:1511	highest in the moss crusts	1486:1511	highest in the moss crusts	1486:1511	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	9	71	theme	deeper	1650:1655	arg1	comprehension					1657:1669	a deeper comprehension	1648:1669	a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs	1648:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	2	72	theme	community	321:329	arg1	characteristics					349:363	bacterial community and physiological characteristics	311:363	bacterial community and physiological characteristics	311:363	However, it is unclear how bacterial community and physiological characteristics vary along the BSCs successional stages.
37147551	8	73	theme	crusts	1344:1349	arg1	types					1335:1339	the cyanobacterial crusts than other types	1298:1339	the cyanobacterial crusts than other types of crusts	1298:1349	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	0	74	theme	CO2	72:74	arg1	exchange					76:83	CO2 exchange	72:83	CO2 exchange	72:83	Changes of bacterial community structure,monosaccharide composition and CO2 exchange along the successional stages of biological soil crusts.
37147551	9	75	from	BSCs	1781:1784	arg1	networks					1744:1751	the networks	1740:1751	the networks of bacterial communities in BSCs	1740:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	9	75	from	BSCs	1781:1784	arg1	components					1718:1727	the monosaccharide components	1699:1727	the monosaccharide components of EPS	1699:1734	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
37147551	8	76	theme	fucose	1374:1379	arg1	contents					1362:1369	the contents	1358:1369	the contents of fucose, xylose, mannose and glucose	1358:1408	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	8	76	theme	fucose	1374:1379	arg1	highest					1419:1425	highest	1419:1425	highest	1419:1425	Specifically, the contents of rhamnose and arabinose were higher in the cyanobacterial crusts than other types of crusts, while the contents of fucose, xylose, mannose and glucose were the highest in cyanobacterial-lichen crusts, and galactose content was highest in the moss crusts.
37147551	1	77	theme	soil	153:156	arg1	crusts					158:163	Biological soil crusts	142:163	Biological soil crusts (BSCs)	142:170	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	1	77	theme	soil	153:156	arg1	landscape					198:206	a dominant ecological landscape	176:206	a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux	176:281	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	1	77	theme	soil	153:156	arg1	BSCs					166:169	BSCs	166:169	BSCs	166:169	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	3	78	theme	extracellular	519:531	arg1	polysaccharides					533:547	extracellular polysaccharides	519:547	extracellular polysaccharides (EPSs)	519:554	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	3	78	theme	extracellular	519:531	arg1	EPSs					550:553	EPSs	550:553	EPSs	550:553	In this study, bacterial community composition, physiological characteristics, and monosaccharide composition of extracellular polysaccharides (EPSs) were compared among different successional stages.
37147551	1	79	theme	a significant	232:244	arg1	impact					246:251	a significant impact	232:251	a significant impact	232:251	Biological soil crusts (BSCs) are a dominant ecological landscape of drylands, which have a significant impact on global biogeochemical flux.
37147551	4	80	theme	bacterial	659:667	arg1	species					669:675	dominant bacterial species	650:675	dominant bacterial species	650:675	Our findings demonstrated that besides the dominant bacterial species, the bacterial communities also showed considerable differences between these two stages.
37147551	7	81	theme	EPSs	1162:1165	arg1	components					1167:1176	the EPSs components	1158:1176	the EPSs components	1158:1176	The monosaccharide analysis indicated that the EPSs components also varied depending on BSCs' successional stages.
37147551	9	82	from	components	1718:1727	arg1	BSCs					1781:1784	BSCs	1781:1784	BSCs	1781:1784	Altogether, our results stress the heterogeneous variation of BSCs along with succession, and this work offered a fresh viewpoint for a deeper comprehension of the interactions between the monosaccharide components of EPS and the networks of bacterial communities in BSCs.
36230221	0	0	theme	Coffee	69:74	arg1	Composition					32:42	Composition	32:42	Composition	32:42	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	0	0	theme	Coffee	69:74	arg1	Analysis					0:7	Analysis	0:7	Analysis of Volatile Compounds	0:29	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	0	0	theme	Coffee	69:74	arg1	Behavior					57:64	Thermal Behavior	49:64	Thermal Behavior of Coffee	49:74	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	3	1	theme	coffee	578:583	arg1	type					585:588	coffee type	578:588	coffee type	578:588	The results showed that volatile compounds are released differently in coffee depending on coffee type and degree of roasting.
36230221	0	2	theme	Volatile	12:19	arg1	Compounds					21:29	Volatile Compounds	12:29	Volatile Compounds	12:29	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	1	3	theme	volatile	216:223	arg1	contents					234:241	volatile compound contents	216:241	volatile compound contents of roasted coffee	216:259	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	2	4	theme	lipids	452:457	arg1	composition					373:383	coffee composition	366:383	coffee composition	366:383	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	4	theme	lipids	452:457	arg1	drying					394:399	drying	394:399	drying	394:399	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	4	theme	lipids	452:457	arg1	loss					408:411	water loss	402:411	water loss	402:411	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	4	theme	lipids	452:457	arg1	decomposition					418:430	decomposition	418:430	decomposition	418:430	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	6	5	theme	Robusta	1036:1042	arg1	variety					1044:1050	The Robusta variety	1032:1050	The Robusta variety	1032:1050	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	4	6	from	present	651:657	arg1	samples					666:672	the samples	662:672	the samples	662:672	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	1	7	theme	compound	225:232	arg1	contents					234:241	volatile compound contents	216:241	volatile compound contents of roasted coffee	216:259	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	1	8	theme	contents	234:241	arg1	beans					261:265	the thermal behavior, composition, and volatile compound contents of roasted coffee beans	177:265	the thermal behavior, composition, and volatile compound contents of roasted coffee beans	177:265	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	2	9	theme	proteins	477:484	arg1	composition					373:383	coffee composition	366:383	coffee composition	366:383	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	9	theme	proteins	477:484	arg1	drying					394:399	drying	394:399	drying	394:399	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	9	theme	proteins	477:484	arg1	loss					408:411	water loss	402:411	water loss	402:411	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	9	theme	proteins	477:484	arg1	decomposition					418:430	decomposition	418:430	decomposition	418:430	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	5	10	dep	g	885:885	arg1	acid/kg					894:900	gallic acid/kg	887:900	13.3 and 18.9 g gallic acid/kg	871:900	The total polyphenol contents ranged between 13.3 and 18.9 g gallic acid/kg, being slightly higher in Robusta than in Arabica varieties and in more intensely roasted beans compared to medium-roasted beans.
36230221	4	11	theme	methyl	713:718	arg1	formate					720:726	methyl formate	713:726	methyl formate	713:726	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	6	12	theme	mineral	1063:1069	arg1	contents					1071:1078	higher mineral contents	1056:1078	higher mineral contents	1056:1078	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	4	13	attach	present	651:657	arg2	compounds					641:649	The most abundant volatile compounds	614:649	The most abundant volatile compounds present in the samples	614:672	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	13	attach	present	651:657	arg1	samples					666:672	the samples	662:672	the samples	662:672	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	13	attach	present	651:657	arg2	2-methylfuran					698:710	2-methylfuran	698:710	2-methylfuran	698:710	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	7	14	theme	polyphenol	1294:1303	arg1	contents					1305:1312	mineral and polyphenol contents	1282:1312	contents	1305:1312	Discrimination between coffee varieties and roasting intensities is possible based on mineral and polyphenol contents.
36230221	6	15	theme	most	1114:1117	arg1	Ca					1132:1133	Ca	1132:1133	Ca	1132:1133	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	Cu					1144:1145	Cu	1144:1145	Cu	1144:1145	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	P					1148:1148	P	1148:1148	P	1148:1148	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	minerals					1119:1126	most minerals	1114:1126	most minerals (K, Ca, Mg, Fe, Cu, P, N, and S)	1114:1159	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	Mg					1136:1137	Mg	1136:1137	Mg	1136:1137	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	Fe					1140:1141	Fe	1140:1141	Fe	1140:1141	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	N					1151:1151	N	1151:1151	N	1151:1151	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	K					1129:1129	K	1129:1129	K	1129:1129	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	15	theme	most	1114:1117	arg1	S					1158:1158	S	1158:1158	S	1158:1158	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	4	16	theme	volatile	632:639	arg1	2-methylfuran					698:710	2-methylfuran	698:710	2-methylfuran	698:710	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	16	theme	volatile	632:639	arg1	compounds					641:649	The most abundant volatile compounds	614:649	The most abundant volatile compounds present in the samples	614:672	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	17	theme	5-methyl	786:793	arg1	furfural					795:802	5-methyl furfural	786:802	5-methyl furfural	786:802	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	5	18	theme	Arabica	944:950	arg1	varieties					952:960	Arabica varieties	944:960	Arabica varieties	944:960	The total polyphenol contents ranged between 13.3 and 18.9 g gallic acid/kg, being slightly higher in Robusta than in Arabica varieties and in more intensely roasted beans compared to medium-roasted beans.
36230221	5	19	theme	gallic	887:892	arg1	acid/kg					894:900	gallic acid/kg	887:900	13.3 and 18.9 g gallic acid/kg	871:900	The total polyphenol contents ranged between 13.3 and 18.9 g gallic acid/kg, being slightly higher in Robusta than in Arabica varieties and in more intensely roasted beans compared to medium-roasted beans.
36230221	2	20	theme	coffee	366:371	arg1	composition					373:383	coffee composition	366:383	coffee composition	366:383	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	1	21	theme	roasted	246:252	arg1	coffee					254:259	roasted coffee	246:259	roasted coffee	246:259	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	2	22	from	transformations	347:361	arg1	composition					373:383	coffee composition	366:383	coffee composition	366:383	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	22	from	transformations	347:361	arg1	drying					394:399	drying	394:399	drying	394:399	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	22	from	transformations	347:361	arg1	loss					408:411	water loss	402:411	water loss	402:411	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	22	from	transformations	347:361	arg1	decomposition					418:430	decomposition	418:430	decomposition	418:430	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	4	23	dep	2-butanone	679:688	arg1	furan					691:695	furan	691:695	furan	691:695	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	24	theme	abundant	623:630	arg1	2-methylfuran					698:710	2-methylfuran	698:710	2-methylfuran	698:710	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	24	theme	abundant	623:630	arg1	compounds					641:649	The most abundant volatile compounds	614:649	The most abundant volatile compounds present in the samples	614:672	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	0	25	theme	Compounds	21:29	arg1	Composition					32:42	Composition	32:42	Composition	32:42	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	0	25	theme	Compounds	21:29	arg1	Analysis					0:7	Analysis	0:7	Analysis of Volatile Compounds	0:29	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	0	25	theme	Compounds	21:29	arg1	Behavior					57:64	Thermal Behavior	49:64	Thermal Behavior of Coffee	49:74	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	1	26	theme	coffee	254:259	arg1	contents					234:241	volatile compound contents	216:241	volatile compound contents of roasted coffee	216:259	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	1	26	theme	coffee	254:259	arg1	composition					199:209	composition	199:209	composition	199:209	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	1	26	theme	coffee	254:259	arg1	behavior					189:196	thermal behavior	181:196	thermal behavior	181:196	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	0	27	theme	Roasting	107:114	arg1	Intensity					116:124	Roasting Intensity	107:124	Roasting Intensity	107:124	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	3	28	theme	volatile	511:518	arg1	compounds					520:528	volatile compounds	511:528	volatile compounds	511:528	The results showed that volatile compounds are released differently in coffee depending on coffee type and degree of roasting.
36230221	2	29	theme	water	402:406	arg1	loss					408:411	water loss	402:411	water loss	402:411	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	3	30	theme	roasting	604:611	arg1	degree					594:599	degree	594:599	degree	594:599	The results showed that volatile compounds are released differently in coffee depending on coffee type and degree of roasting.
36230221	3	30	theme	roasting	604:611	arg1	type					585:588	coffee type	578:588	coffee type	578:588	The results showed that volatile compounds are released differently in coffee depending on coffee type and degree of roasting.
36230221	7	31	theme	mineral	1282:1288	arg1	contents					1305:1312	mineral and polyphenol contents	1282:1312	contents	1305:1312	Discrimination between coffee varieties and roasting intensities is possible based on mineral and polyphenol contents.
36230221	5	32	theme	polyphenol	836:845	arg1	contents					847:854	The total polyphenol contents	826:854	The total polyphenol contents	826:854	The total polyphenol contents ranged between 13.3 and 18.9 g gallic acid/kg, being slightly higher in Robusta than in Arabica varieties and in more intensely roasted beans compared to medium-roasted beans.
36230221	5	33	theme	total	830:834	arg1	contents					847:854	The total polyphenol contents	826:854	The total polyphenol contents	826:854	The total polyphenol contents ranged between 13.3 and 18.9 g gallic acid/kg, being slightly higher in Robusta than in Arabica varieties and in more intensely roasted beans compared to medium-roasted beans.
36230221	1	34	theme	thermal	181:187	arg1	behavior					189:196	thermal behavior	181:196	thermal behavior	181:196	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	2	35	theme	polysaccharides	435:449	arg1	composition					373:383	coffee composition	366:383	coffee composition	366:383	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	35	theme	polysaccharides	435:449	arg1	drying					394:399	drying	394:399	drying	394:399	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	35	theme	polysaccharides	435:449	arg1	loss					408:411	water loss	402:411	water loss	402:411	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	35	theme	polysaccharides	435:449	arg1	decomposition					418:430	decomposition	418:430	decomposition	418:430	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	1	36	theme	behavior	189:196	arg1	beans					261:265	the thermal behavior, composition, and volatile compound contents of roasted coffee beans	177:265	the thermal behavior, composition, and volatile compound contents of roasted coffee beans	177:265	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	5	37	theme	medium-roasted	1010:1023	arg1	beans					1025:1029	medium-roasted beans	1010:1029	medium-roasted beans	1010:1029	The total polyphenol contents ranged between 13.3 and 18.9 g gallic acid/kg, being slightly higher in Robusta than in Arabica varieties and in more intensely roasted beans compared to medium-roasted beans.
36230221	6	38	theme	roasting	1176:1183	arg1	intensity					1185:1193	roasting intensity	1176:1193	roasting intensity	1176:1193	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	0	39	theme	Thermal	49:55	arg1	Behavior					57:64	Thermal Behavior	49:64	Thermal Behavior of Coffee	49:74	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	7	40	theme	coffee	1219:1224	arg1	varieties					1226:1234	coffee varieties	1219:1234	coffee varieties	1219:1234	Discrimination between coffee varieties and roasting intensities is possible based on mineral and polyphenol contents.
36230221	6	41	theme	higher	1056:1061	arg1	contents					1071:1078	higher mineral contents	1056:1078	higher mineral contents	1056:1078	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	2	42	theme	thermal	313:319	arg1	analysis					321:328	The thermal analysis	309:328	The thermal analysis	309:328	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	6	43	theme	minerals	1119:1126	arg1	contents					1102:1109	the contents	1098:1109	the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S)	1098:1159	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	5	44	theme	roasted	984:990	arg1	beans					992:996	more intensely roasted beans	969:996	more intensely roasted beans	969:996	The total polyphenol contents ranged between 13.3 and 18.9 g gallic acid/kg, being slightly higher in Robusta than in Arabica varieties and in more intensely roasted beans compared to medium-roasted beans.
36230221	1	45	theme	roasting	289:296	arg1	intensity					298:306	roasting intensity	289:306	roasting intensity	289:306	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	6	46	contain	has	1052:1054	arg2	contents					1071:1078	higher mineral contents	1056:1078	higher mineral contents	1056:1078	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	46	contain	has	1052:1054	arg1	variety					1044:1050	The Robusta variety	1032:1050	The Robusta variety	1032:1050	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	7	47	theme	roasting	1240:1247	arg1	intensities					1249:1259	roasting intensities	1240:1259	roasting intensities	1240:1259	Discrimination between coffee varieties and roasting intensities is possible based on mineral and polyphenol contents.
36230221	4	48	theme	2-butanone	679:688	arg1	2-methylfuran					698:710	2-methylfuran	698:710	2-methylfuran	698:710	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	48	theme	2-butanone	679:688	arg1	compounds					641:649	The most abundant volatile compounds	614:649	The most abundant volatile compounds present in the samples	614:672	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	1	49	theme	composition	199:209	arg1	beans					261:265	the thermal behavior, composition, and volatile compound contents of roasted coffee beans	177:265	the thermal behavior, composition, and volatile compound contents of roasted coffee beans	177:265	This study aimed to investigate the ways in which the thermal behavior, composition, and volatile compound contents of roasted coffee beans depend on variety and roasting intensity.
36230221	2	50	theme	acids	466:470	arg1	composition					373:383	coffee composition	366:383	coffee composition	366:383	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	50	theme	acids	466:470	arg1	drying					394:399	drying	394:399	drying	394:399	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	50	theme	acids	466:470	arg1	loss					408:411	water loss	402:411	water loss	402:411	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	2	50	theme	acids	466:470	arg1	decomposition					418:430	decomposition	418:430	decomposition	418:430	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	6	51	dep	minerals	1119:1126	arg1	Ca					1132:1133	Ca	1132:1133	Ca	1132:1133	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	Cu					1144:1145	Cu	1144:1145	Cu	1144:1145	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	P					1148:1148	P	1148:1148	P	1148:1148	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	minerals					1119:1126	most minerals	1114:1126	most minerals (K, Ca, Mg, Fe, Cu, P, N, and S)	1114:1159	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	Mg					1136:1137	Mg	1136:1137	Mg	1136:1137	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	Fe					1140:1141	Fe	1140:1141	Fe	1140:1141	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	N					1151:1151	N	1151:1151	N	1151:1151	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	K					1129:1129	K	1129:1129	K	1129:1129	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	6	51	dep	minerals	1119:1126	arg1	S					1158:1158	S	1158:1158	S	1158:1158	The Robusta variety has higher mineral contents than Arabica, and the contents of most minerals (K, Ca, Mg, Fe, Cu, P, N, and S) increased with roasting intensity.
36230221	4	52	theme	acetic	763:768	arg1	acid					770:773	acetic acid	763:773	acetic acid	763:773	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	53	theme	present	651:657	arg1	2-methylfuran					698:710	2-methylfuran	698:710	2-methylfuran	698:710	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	53	theme	present	651:657	arg1	compounds					641:649	The most abundant volatile compounds	614:649	The most abundant volatile compounds present in the samples	614:672	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	4	54	from	samples	666:672	arg1	present					651:657	present	651:657	present	651:657	The most abundant volatile compounds present in the samples were 2-butanone, furan, 2-methylfuran, methyl formate, 2.3-pentanedione, methylpyrazine, acetic acid, furfural, 5-methyl furfural, and 2-furanmethanol.
36230221	2	55	theme	amino	460:464	arg1	acids					466:470	amino acids	460:470	amino acids	460:470	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36230221	0	56	dep	Analysis	0:7	arg1	Beans					76:80	Beans	76:80	Beans	76:80	Analysis of Volatile Compounds, Composition, and Thermal Behavior of Coffee Beans According to Variety and Roasting Intensity.
36230221	2	57	theme	various	339:345	arg1	transformations					347:361	various transformations	339:361	various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins	339:484	The thermal analysis revealed various transformations in coffee composition, namely, drying, water loss, and decomposition of polysaccharides, lipids, amino acids, and proteins.
36832934	14	0	theme	acid	1294:1297	arg1	amounts					1264:1270	the relative amounts	1251:1270	the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+)	1251:1363	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	0	theme	acid	1294:1297	arg1	acid					1294:1297	gamma-aminobutyric acid	1275:1297	gamma-aminobutyric acid (GABA)	1275:1304	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	0	theme	acid	1294:1297	arg1	dinucleotide					1345:1356	nicotinamide and nicotinamide adenine dinucleotide	1307:1356	dinucleotide	1345:1356	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	0	theme	acid	1294:1297	arg1	GABA					1300:1303	GABA	1300:1303	GABA	1300:1303	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	1	theme	relative	1255:1262	arg1	amounts					1264:1270	the relative amounts	1251:1270	the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+)	1251:1363	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	1	theme	relative	1255:1262	arg1	acid					1294:1297	gamma-aminobutyric acid	1275:1297	gamma-aminobutyric acid (GABA)	1275:1304	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	1	theme	relative	1255:1262	arg1	dinucleotide					1345:1356	nicotinamide and nicotinamide adenine dinucleotide	1307:1356	dinucleotide	1345:1356	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	1	theme	relative	1255:1262	arg1	GABA					1300:1303	GABA	1300:1303	GABA	1300:1303	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	13	2	theme	acids	1140:1144	arg1	metabolism					1081:1090	the metabolism	1077:1090	the metabolism	1077:1090	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	1	3	theme	Agaricus	152:159	arg1	ABP					186:188	ABP	186:188	ABP	186:188	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	1	3	theme	Agaricus	152:159	arg1	polysaccharide					170:183	Agaricus bisporus polysaccharide	152:183	Agaricus bisporus polysaccharide (ABP)	152:189	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	1	3	theme	Agaricus	152:159	arg1	component					214:222	an important active component	194:222	an important active component in edible mushrooms	194:242	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	14	4	theme	24	1232:1233	arg1	h					1235:1235	h	1235:1235	h	1235:1235	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	0	5	theme	In	75:76	arg1	Fermentation					84:95	In Vitro Fermentation	75:95	In Vitro Fermentation	75:95	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	3	6	theme	fermentation	655:666	arg1	h					641:641	24 h	638:641	24 h of in vitro fermentation	638:666	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	14	7	theme	15.36-fold	1388:1397	arg1	increases					1399:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	1	8	theme	bisporus	161:168	arg1	ABP					186:188	ABP	186:188	ABP	186:188	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	1	8	theme	bisporus	161:168	arg1	polysaccharide					170:183	Agaricus bisporus polysaccharide	152:183	Agaricus bisporus polysaccharide (ABP)	152:189	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	1	8	theme	bisporus	161:168	arg1	component					214:222	an important active component	194:222	an important active component in edible mushrooms	194:242	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	0	9	from	Effect	0:5	arg1	Microbiota					57:66	Human Gut Microbiota	47:66	Human Gut Microbiota during In Vitro Fermentation	47:95	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	3	10	dep	in	646:647	arg1	vitro					649:653	vitro	649:653	vitro	649:653	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	18	11	theme	dietary	1644:1650	arg1	supplement					1652:1661	a potential prebiotic or dietary supplement	1619:1661	supplement	1652:1661	These results laid the research foundation for exploring ABP as a potential prebiotic or dietary supplement for the targeted regulation of gut microbiota or metabolites.
36832934	13	12	theme	lipids	1123:1128	arg1	metabolism					1081:1090	the metabolism	1077:1090	the metabolism	1077:1090	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	4	13	theme	short-chain	673:683	arg1	acids					691:695	short-chain fatty acids	673:695	The short-chain fatty acids (SCFAs) content	669:711	The short-chain fatty acids (SCFAs) content also increased more than 15-fold, accordingly.
36832934	4	13	theme	short-chain	673:683	arg1	SCFAs					698:702	SCFAs	698:702	SCFAs	698:702	The short-chain fatty acids (SCFAs) content also increased more than 15-fold, accordingly.
36832934	5	14	theme	relative	796:803	arg1	abundance					805:813	the relative abundance	792:813	the relative abundance of Bacteroides (Ba	792:832	Moreover, the effects of ABP on the relative abundance of Bacteroides (Ba.)
36832934	0	15	theme	Integrative	101:111	arg1	Analysis					113:120	An Integrative Analysis	98:120	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.	0:150	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	2	16	dep	in	409:410	arg1	vitro					412:416	vitro	412:416	vitro	412:416	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	3	17	theme	main	442:445	arg1	bacteria					457:464	The main degrading bacteria	438:464	The main degrading bacteria for ABP	438:472	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	3	17	theme	main	442:445	arg1	Streptococcus					492:504	Streptococcus	492:504	Streptococcus	492:504	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	14	18	contain	had	1365:1367	arg1	amounts					1264:1270	the relative amounts	1251:1270	the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+)	1251:1363	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	18	contain	had	1365:1367	arg2	increases					1399:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	18	contain	had	1365:1367	arg1	GABA					1300:1303	GABA	1300:1303	GABA	1300:1303	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	18	contain	had	1365:1367	arg1	dinucleotide					1345:1356	nicotinamide and nicotinamide adenine dinucleotide	1307:1356	dinucleotide	1345:1356	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	18	contain	had	1365:1367	arg1	acid					1294:1297	gamma-aminobutyric acid	1275:1297	gamma-aminobutyric acid (GABA)	1275:1304	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	18	19	theme	research	1578:1585	arg1	foundation					1587:1596	the research foundation	1574:1596	the research foundation for exploring ABP as a potential prebiotic or dietary supplement for the targeted regulation of gut microbiota or metabolites	1574:1722	These results laid the research foundation for exploring ABP as a potential prebiotic or dietary supplement for the targeted regulation of gut microbiota or metabolites.
36832934	13	20	theme	amino	1134:1138	arg1	acids					1140:1144	amino acids	1134:1144	amino acids	1134:1144	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	9	21	dep	Ba	945:946	arg1	thetaiotaomicron					927:942	thetaiotaomicron	927:942	thetaiotaomicron	927:942	thetaiotaomicron, Ba.
36832934	7	22	theme	species	869:875	arg1	level					877:881	the species level	865:881	the species level	865:881	at the species level were further determined.
36832934	14	23	theme	adenine	1337:1343	arg1	acid					1294:1297	gamma-aminobutyric acid	1275:1297	gamma-aminobutyric acid (GABA)	1275:1304	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	23	theme	adenine	1337:1343	arg1	NAD+					1359:1362	NAD+	1359:1362	NAD+	1359:1362	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	23	theme	adenine	1337:1343	arg1	dinucleotide					1345:1356	nicotinamide and nicotinamide adenine dinucleotide	1307:1356	dinucleotide	1345:1356	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	3	24	dep	Megamonas	567:575	arg1	Bifidobacterium					537:551	Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas	507:575	Bifidobacterium	537:551	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	3	24	dep	Megamonas	567:575	arg1	Paraprevotella					521:534	Paraprevotella	521:534	Paraprevotella	521:534	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	3	24	dep	Megamonas	567:575	arg1	Lactococcus					554:564	Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas	507:575	Lactococcus	554:564	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	2	25	theme	batch	418:422	arg1	fermentation					424:435	in vitro batch fermentation	409:435	in vitro batch fermentation	409:435	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	5	26	from	effects	774:780	arg1	abundance					805:813	the relative abundance	792:813	the relative abundance of Bacteroides (Ba	792:832	Moreover, the effects of ABP on the relative abundance of Bacteroides (Ba.)
36832934	2	27	from	effect	333:338	arg1	metabolites					370:380	metabolites	370:380	metabolites	370:380	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	2	27	from	effect	333:338	arg1	composition					354:364	composition	354:364	composition	354:364	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	14	28	theme	nicotinamide	1324:1335	arg1	acid					1294:1297	gamma-aminobutyric acid	1275:1297	gamma-aminobutyric acid (GABA)	1275:1304	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	28	theme	nicotinamide	1324:1335	arg1	NAD+					1359:1362	NAD+	1359:1362	NAD+	1359:1362	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	28	theme	nicotinamide	1324:1335	arg1	dinucleotide					1345:1356	nicotinamide and nicotinamide adenine dinucleotide	1307:1356	dinucleotide	1345:1356	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	29	theme	gamma-aminobutyric	1275:1292	arg1	acid					1294:1297	gamma-aminobutyric acid	1275:1297	gamma-aminobutyric acid (GABA)	1275:1304	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	29	theme	gamma-aminobutyric	1275:1292	arg1	dinucleotide					1345:1356	nicotinamide and nicotinamide adenine dinucleotide	1307:1356	dinucleotide	1345:1356	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	29	theme	gamma-aminobutyric	1275:1292	arg1	GABA					1300:1303	GABA	1300:1303	GABA	1300:1303	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	0	30	dep	In	75:76	arg1	Vitro					78:82	Vitro	78:82	Vitro	78:82	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	5	31	theme	Ba	831:832	arg1	abundance					805:813	the relative abundance	792:813	the relative abundance of Bacteroides (Ba	792:832	Moreover, the effects of ABP on the relative abundance of Bacteroides (Ba.)
36832934	0	32	theme	bisporus	19:26	arg1	Polysaccharides					28:42	Agaricus bisporus Polysaccharides	10:42	Agaricus bisporus Polysaccharides	10:42	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	1	33	theme	gut	270:272	arg1	microbiota					274:283	gut microbiota	270:283	gut microbiota	270:283	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	13	34	theme	ABP	1043:1045	arg1	catabolism					1029:1038	the catabolism	1025:1038	the catabolism of ABP	1025:1045	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	14	35	theme	nicotinamide	1307:1318	arg1	acid					1294:1297	gamma-aminobutyric acid	1275:1297	gamma-aminobutyric acid (GABA)	1275:1304	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	35	theme	nicotinamide	1307:1318	arg1	NAD+					1359:1362	NAD+	1359:1362	NAD+	1359:1362	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	14	35	theme	nicotinamide	1307:1318	arg1	dinucleotide					1345:1356	nicotinamide and nicotinamide adenine dinucleotide	1307:1356	dinucleotide	1345:1356	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	2	36	theme	microbiota	395:404	arg1	metabolites					370:380	metabolites	370:380	metabolites	370:380	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	2	36	theme	microbiota	395:404	arg1	composition					354:364	composition	354:364	composition	354:364	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	0	37	theme	Agaricus	10:17	arg1	Polysaccharides					28:42	Agaricus bisporus Polysaccharides	10:42	Agaricus bisporus Polysaccharides	10:42	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	18	38	theme	targeted	1671:1678	arg1	regulation					1680:1689	the targeted regulation	1667:1689	the targeted regulation of gut microbiota or metabolites	1667:1722	These results laid the research foundation for exploring ABP as a potential prebiotic or dietary supplement for the targeted regulation of gut microbiota or metabolites.
36832934	18	39	theme	gut	1694:1696	arg1	microbiota					1698:1707	gut microbiota	1694:1707	gut microbiota	1694:1707	These results laid the research foundation for exploring ABP as a potential prebiotic or dietary supplement for the targeted regulation of gut microbiota or metabolites.
36832934	2	40	theme	gut	391:393	arg1	microbiota					395:404	human gut microbiota	385:404	human gut microbiota	385:404	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	15	41	dep	Ba	1492:1493	arg1	thetaiotaomicron					1474:1489	thetaiotaomicron	1474:1489	thetaiotaomicron	1474:1489	thetaiotaomicron, Ba.
36832934	2	42	theme	human	385:389	arg1	microbiota					395:404	human gut microbiota	385:404	human gut microbiota	385:404	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	17	43	theme	|||r|||	1542:1544	arg1	longum					1534:1539	longum	1534:1539	|longum (||r|| > 0.98).|	1534:1553	|longum (||r|| > 0.98).|
36832934	17	43	theme	|||r|||	1542:1544	arg1	>					1546:1546	|||r|| > 0.98|	1542:1551	|||r|| > 0.98|	1542:1551	|longum (||r|| > 0.98).|
36832934	1	44	theme	important	197:205	arg1	component					214:222	an important active component	194:222	an important active component in edible mushrooms	194:242	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	1	44	theme	important	197:205	arg1	polysaccharide					170:183	Agaricus bisporus polysaccharide	152:183	Agaricus bisporus polysaccharide (ABP)	152:189	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	0	45	theme	Polysaccharides	28:42	arg1	Effect					0:5	Effect	0:5	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.	0:150	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	0	46	theme	Microbiome	125:134	arg1	Analysis					113:120	An Integrative Analysis	98:120	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.	0:150	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	2	47	theme	in	409:410	arg1	fermentation					424:435	in vitro batch fermentation	409:435	in vitro batch fermentation	409:435	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	13	48	theme	carbohydrates	1095:1107	arg1	metabolism					1081:1090	the metabolism	1077:1090	the metabolism	1077:1090	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	1	49	theme	active	207:212	arg1	component					214:222	an important active component	194:222	an important active component in edible mushrooms	194:242	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	1	49	theme	active	207:212	arg1	polysaccharide					170:183	Agaricus bisporus polysaccharide	152:183	Agaricus bisporus polysaccharide (ABP)	152:189	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	0	50	theme	Gut	53:55	arg1	Microbiota					57:66	Human Gut Microbiota	47:66	Human Gut Microbiota during In Vitro Fermentation	47:95	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	18	51	theme	microbiota	1698:1707	arg1	regulation					1680:1689	the targeted regulation	1667:1689	the targeted regulation of gut microbiota or metabolites	1667:1722	These results laid the research foundation for exploring ABP as a potential prebiotic or dietary supplement for the targeted regulation of gut microbiota or metabolites.
36832934	5	52	theme	ABP	785:787	arg1	effects					774:780	the effects	770:780	the effects of ABP on the relative abundance of Bacteroides (Ba	770:832	Moreover, the effects of ABP on the relative abundance of Bacteroides (Ba.)
36832934	1	53	with	interaction	253:263	arg1	microbiota					274:283	gut microbiota	270:283	gut microbiota	270:283	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	4	54	theme	acids	691:695	arg1	content					705:711	The short-chain fatty acids (SCFAs) content	669:711	The short-chain fatty acids (SCFAs) content	669:711	The short-chain fatty acids (SCFAs) content also increased more than 15-fold, accordingly.
36832934	0	55	theme	Human	47:51	arg1	Microbiota					57:66	Human Gut Microbiota	47:66	Human Gut Microbiota during In Vitro Fermentation	47:95	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	0	56	theme	Metabolome	140:149	arg1	Analysis					113:120	An Integrative Analysis	98:120	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.	0:150	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	3	57	theme	in	646:647	arg1	fermentation					655:666	in vitro fermentation	646:666	in vitro fermentation	646:666	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	13	58	theme	nucleotides	1110:1120	arg1	metabolism					1081:1090	the metabolism	1077:1090	the metabolism	1077:1090	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	4	59	theme	fatty	685:689	arg1	acids					691:695	short-chain fatty acids	673:695	The short-chain fatty acids (SCFAs) content	669:711	The short-chain fatty acids (SCFAs) content also increased more than 15-fold, accordingly.
36832934	4	59	theme	fatty	685:689	arg1	SCFAs					698:702	SCFAs	698:702	SCFAs	698:702	The short-chain fatty acids (SCFAs) content also increased more than 15-fold, accordingly.
36832934	13	60	theme	PICRUSt	994:1000	arg1	analysis					1002:1009	PICRUSt analysis	994:1009	PICRUSt analysis	994:1009	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	1	61	from	component	214:222	arg1	mushrooms					234:242	edible mushrooms	227:242	edible mushrooms	227:242	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	6	62	dep	Bifidobacterium	840:854	arg1	Bi					857:858	Bi	857:858	Bi.	857:859	and Bifidobacterium (Bi.)
36832934	2	63	dep	composition	354:364	arg1	the					350:352	the	350:352	the	350:352	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	3	64	theme	degrading	447:455	arg1	bacteria					457:464	The main degrading bacteria	438:464	The main degrading bacteria for ABP	438:472	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	3	64	theme	degrading	447:455	arg1	Streptococcus					492:504	Streptococcus	492:504	Streptococcus	492:504	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	14	65	theme	h	1235:1235	arg1	fermentation					1237:1248	24 h fermentation	1232:1248	24 h fermentation	1232:1248	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	13	66	theme	metabonomic	1176:1186	arg1	results					1188:1194	metabonomic results	1176:1194	metabonomic results	1176:1194	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	3	67	theme	relative	601:608	arg1	abundances					610:619	relative abundances	601:619	relative abundances	601:619	The main degrading bacteria for ABP were Bacteroides, Streptococcus, Enterococcus, Paraprevotella, Bifidobacterium, Lactococcus, Megamonas, and Eubacterium, whose relative abundances increased during 24 h of in vitro fermentation.
36832934	13	68	from	changes	1066:1072	arg1	metabolism					1081:1090	the metabolism	1077:1090	the metabolism	1077:1090	PICRUSt analysis revealed that the catabolism of ABP was accompanied by changes in the metabolism of carbohydrates, nucleotides, lipids and amino acids, which were also supported by metabonomic results.
36832934	14	69	theme	11.34-	1377:1382	arg1	increases					1399:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	18	70	theme	metabolites	1712:1722	arg1	regulation					1680:1689	the targeted regulation	1667:1689	the targeted regulation of gut microbiota or metabolites	1667:1722	These results laid the research foundation for exploring ABP as a potential prebiotic or dietary supplement for the targeted regulation of gut microbiota or metabolites.
36832934	5	71	theme	Bacteroides	818:828	arg1	Ba					831:832	Bacteroides (Ba	818:832	Bacteroides (Ba	818:832	Moreover, the effects of ABP on the relative abundance of Bacteroides (Ba.)
36832934	1	72	theme	edible	227:232	arg1	mushrooms					234:242	edible mushrooms	227:242	edible mushrooms	227:242	Agaricus bisporus polysaccharide (ABP) is an important active component in edible mushrooms, but its interaction with gut microbiota is unclear.
36832934	10	73	dep	Ba	963:964	arg1	intestinalis					949:960	intestinalis	949:960	intestinalis	949:960	intestinalis, Ba.
36832934	0	74	dep	Effect	0:5	arg1	Analysis					113:120	An Integrative Analysis	98:120	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.	0:150	Effect of Agaricus bisporus Polysaccharides on Human Gut Microbiota during In Vitro Fermentation: An Integrative Analysis of Microbiome and Metabolome.
36832934	14	75	theme	Bacteroides	1457:1467	arg1	Ba					1470:1471	Bacteroides (Ba	1457:1471	Bacteroides (Ba	1457:1471	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
36832934	2	76	theme	ABP	343:345	arg1	effect					333:338	the effect	329:338	the effect of ABP on the composition and metabolites of human gut microbiota	329:404	Therefore, this study evaluated the effect of ABP on the composition and metabolites of human gut microbiota by in vitro batch fermentation.
36832934	14	77	theme	14.43-	1369:1374	arg1	increases					1399:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	14.43-, 11.34- and 15.36-fold increases	1369:1407	It is worth mentioning that, after 24 h fermentation, the relative amounts of gamma-aminobutyric acid (GABA), nicotinamide and nicotinamide adenine dinucleotide (NAD+) had 14.43-, 11.34- and 15.36-fold increases, respectively, which were positively related to Bacteroides (Ba.
35281923	0	0	theme	Active	78:83	arg1	APS-II					101:106	the Active Polysaccharides APS-II	74:106	the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase	74:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	6	1	theme	nuclear	860:866	arg1	resonance					877:885	nuclear magnetic resonance	860:885	nuclear magnetic resonance	860:885	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	5	2	theme	degraded	538:545	arg1	products					547:554	The degraded products	534:554	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)]	534:643	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	2	3	theme	APSs	353:356	arg1	APS-Ⅱ					289:293	APS-Ⅱ	289:293	APS-Ⅱ (10 kDa)	289:302	Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs.
35281923	2	3	theme	APSs	353:356	arg1	component					340:348	the main immunologically active component	308:348	the main immunologically active component of APSs	308:356	Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs.
35281923	8	4	link	α-D-1,4-linked	1080:1093	arg1	glucans					1095:1101	α-D-1,4-linked glucans	1080:1101	α-D-1,4-linked glucans	1080:1101	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	8	4	link	α-D-1,4-linked	1080:1093	arg1	backbone					1044:1051	The backbone	1040:1051	The backbone of P4	1040:1057	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	7	5	theme	products	1030:1037	arg1	fractions					1013:1021	the most immunogenic fractions	992:1021	the most immunogenic fractions of the products	992:1037	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	4	6	theme	endo	510:513	arg1	α-1,4-glucosidase					515:531	endo α-1,4-glucosidase	510:531	endo α-1,4-glucosidase	510:531	In this study, APS-II was degraded by endo α-1,4-glucosidase.
35281923	0	7	from	APS-II	101:106	arg1	Components					58:67	Components	58:67	Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase	58:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	0	7	from	APS-II	101:106	arg1	Structure					25:33	Structure	25:33	Structure	25:33	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	0	7	from	APS-II	101:106	arg1	Activity					46:53	Immune Activity	39:53	Immune Activity	39:53	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	7	8	theme	Specific	919:926	arg1	tests					964:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	1	9	theme	Astragali	167:175	arg1	APSs					200:203	APSs	200:203	APSs	200:203	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	1	9	theme	Astragali	167:175	arg1	polysaccharides					183:197	Astragali Radix polysaccharides	167:197	Astragali Radix polysaccharides (APSs)	167:204	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	8	10	theme	α-D-1,4-linked	1080:1093	arg1	glucans					1095:1101	α-D-1,4-linked glucans	1080:1101	α-D-1,4-linked glucans	1080:1101	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	8	10	theme	α-D-1,4-linked	1080:1093	arg1	backbone					1044:1051	The backbone	1040:1051	The backbone of P4	1040:1057	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	9	11	theme	P4	1213:1214	arg1	better					1220:1225	better	1220:1225	better	1220:1225	Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components.
35281923	9	11	theme	P4	1213:1214	arg1	activity					1201:1208	the activity	1197:1208	the activity of P4	1197:1214	Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components.
35281923	6	12	theme	products	750:757	arg1	features					724:731	The structural features	709:731	The structural features of the different products	709:757	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	7	13	theme	non-specific	939:950	arg1	tests					964:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	5	14	with	products	547:554	arg1	degrees					571:577	different degrees	561:577	different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)]	561:643	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	0	15	theme	Polysaccharides	85:99	arg1	APS-II					101:106	the Active Polysaccharides APS-II	74:106	the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase	74:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	6	16	theme	different	740:748	arg1	products					750:757	the different products	736:757	the different products	736:757	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	10	17	theme	quality	1456:1462	arg1	control					1464:1470	quality control	1456:1470	quality control	1456:1470	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	0	18	from	Activity	46:53	arg1	APS-II					101:106	the Active Polysaccharides APS-II	74:106	the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase	74:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	1	19	theme	Radix	177:181	arg1	APSs					200:203	APSs	200:203	APSs	200:203	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	1	19	theme	Radix	177:181	arg1	polysaccharides					183:197	Astragali Radix polysaccharides	167:197	Astragali Radix polysaccharides (APSs)	167:204	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	5	20	theme	polyacrylamide	667:680	arg1	column					701:706	a polyacrylamide gel chromatography column	665:706	a polyacrylamide gel chromatography column	665:706	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	6	21	theme	monosaccharide	788:801	arg1	HPGPC					781:785	HPGPC	781:785	HPGPC	781:785	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	6	21	theme	monosaccharide	788:801	arg1	composition					803:813	monosaccharide composition	788:813	monosaccharide composition	788:813	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	6	22	theme	magnetic	868:875	arg1	resonance					877:885	nuclear magnetic resonance	860:885	nuclear magnetic resonance	860:885	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	3	23	theme	characteristic	372:385	arg1	structure					387:395	the characteristic structure	368:395	the characteristic structure related to activity of APS-Ⅱ	368:424	However, the characteristic structure related to activity of APS-Ⅱ needs further verification and clarification.
35281923	10	24	theme	immune	1353:1358	arg1	activity					1360:1367	the immune activity	1349:1367	the immune activity from the degradation level of polysaccharides	1349:1413	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	5	25	theme	polymerization	582:595	arg1	[1-3					597:600	polymerization [1-3	582:600	polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)]	582:643	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	5	25	theme	polymerization	582:595	arg1	P1					603:604	P1	603:604	P1	603:604	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	7	26	theme	immunogenic	1001:1011	arg1	fractions					1013:1021	the most immunogenic fractions	992:1021	the most immunogenic fractions of the products	992:1037	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	3	27	theme	APS-Ⅱ	420:424	arg1	activity					408:415	activity	408:415	activity of APS-Ⅱ	408:424	However, the characteristic structure related to activity of APS-Ⅱ needs further verification and clarification.
35281923	8	28	theme	C2	1115:1116	arg1	branches					1143:1150	C2 (25.34%) and C6 (34.54%) branches	1115:1150	C2 (25.34%) and C6 (34.54%) branches	1115:1150	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	5	29	theme	[1-3	597:600	arg1	degrees					571:577	different degrees	561:577	different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)]	561:643	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	10	30	theme	APS-Ⅱ	1339:1343	arg1	structure					1326:1334	the structure	1322:1334	the structure of APS-Ⅱ	1322:1343	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	10	30	theme	APS-Ⅱ	1339:1343	arg1	activity					1360:1367	the immune activity	1349:1367	the immune activity from the degradation level of polysaccharides	1349:1413	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	6	31	theme	structural	713:722	arg1	features					724:731	The structural features	709:731	The structural features of the different products	709:757	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	10	32	theme	product	1476:1482	arg1	development					1484:1494	product development	1476:1494	product development	1476:1494	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	0	33	theme	Radix	121:125	arg1	APS-II					101:106	the Active Polysaccharides APS-II	74:106	the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase	74:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	9	34	theme	Immune	1153:1158	arg1	experiments					1170:1180	Immune screening experiments	1153:1180	Immune screening experiments	1153:1180	Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components.
35281923	0	35	theme	Immune	39:44	arg1	Activity					46:53	Immune Activity	39:53	Immune Activity	39:53	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	10	36	theme	degradation	1378:1388	arg1	level					1390:1394	the degradation level	1374:1394	the degradation level of polysaccharides	1374:1413	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	3	37	theme	further	432:438	arg1	verification					440:451	further verification and clarification	432:469	verification	440:451	However, the characteristic structure related to activity of APS-Ⅱ needs further verification and clarification.
35281923	6	38	theme	UPLC-ESI-QTOF-MS	892:907	arg1	analysis					909:916	UPLC-ESI-QTOF-MS analysis	892:916	UPLC-ESI-QTOF-MS analysis	892:916	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	8	39	theme	C6	1131:1132	arg1	branches					1143:1150	C2 (25.34%) and C6 (34.54%) branches	1115:1150	C2 (25.34%) and C6 (34.54%) branches	1115:1150	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	1	40	theme	wide	213:216	arg1	range					218:222	a wide range	211:222	a wide range of biological activities	211:247	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	3	41	theme	related	397:403	arg1	structure					387:395	the characteristic structure	368:395	the characteristic structure related to activity of APS-Ⅱ	368:424	However, the characteristic structure related to activity of APS-Ⅱ needs further verification and clarification.
35281923	9	42	theme	other	1255:1259	arg1	components					1267:1276	the other three components	1251:1276	the other three components	1251:1276	Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components.
35281923	0	43	theme	α-1,4-Glucanase	150:164	arg1	Enzymolysis					130:140	Enzymolysis	130:140	Enzymolysis of Endo α-1,4-Glucanase	130:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	5	44	theme	chromatography	686:699	arg1	column					701:706	a polyacrylamide gel chromatography column	665:706	a polyacrylamide gel chromatography column	665:706	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	8	45	theme	P4	1056:1057	arg1	backbone					1044:1051	The backbone	1040:1051	The backbone of P4	1040:1057	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	8	45	theme	P4	1056:1057	arg1	glucans					1095:1101	α-D-1,4-linked glucans	1080:1101	α-D-1,4-linked glucans	1080:1101	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	10	46	dep	control	1464:1470	arg1	the					1452:1454	the	1452:1454	the	1452:1454	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	6	47	dep	Fourier	816:822	arg1	transform					824:832	transform	824:832	transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis	824:916	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	0	48	theme	Endo	145:148	arg1	α-1,4-Glucanase					150:164	Endo α-1,4-Glucanase	145:164	Endo α-1,4-Glucanase	145:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	9	49	theme	screening	1160:1168	arg1	experiments					1170:1180	Immune screening experiments	1153:1180	Immune screening experiments	1153:1180	Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components.
35281923	5	50	theme	different	561:569	arg1	degrees					571:577	different degrees	561:577	different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)]	561:643	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	2	51	theme	active	333:338	arg1	APS-Ⅱ					289:293	APS-Ⅱ	289:293	APS-Ⅱ (10 kDa)	289:302	Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs.
35281923	2	51	theme	active	333:338	arg1	component					340:348	the main immunologically active component	308:348	the main immunologically active component of APSs	308:356	Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs.
35281923	0	52	dep	Structure	25:33	arg1	the					21:23	the	21:23	the	21:23	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	7	53	theme	immune	928:933	arg1	tests					964:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	8	54	from	branches	1143:1150	arg1	rich					1107:1110	rich	1107:1110	rich	1107:1110	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	10	55	theme	polysaccharides	1399:1413	arg1	level					1390:1394	the degradation level	1374:1394	the degradation level of polysaccharides	1374:1413	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	1	56	contain	have	206:209	arg2	range					218:222	a wide range	211:222	a wide range of biological activities	211:247	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	1	56	contain	have	206:209	arg1	APSs					200:203	APSs	200:203	APSs	200:203	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	1	56	contain	have	206:209	arg1	polysaccharides					183:197	Astragali Radix polysaccharides	167:197	Astragali Radix polysaccharides (APSs)	167:204	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	2	57	theme	preliminary	254:264	arg1	experiment					266:275	Our preliminary experiment	250:275	Our preliminary experiment	250:275	Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs.
35281923	7	58	theme	immune	952:957	arg1	tests					964:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	2	59	theme	main	312:315	arg1	APS-Ⅱ					289:293	APS-Ⅱ	289:293	APS-Ⅱ (10 kDa)	289:302	Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs.
35281923	2	59	theme	main	312:315	arg1	component					340:348	the main immunologically active component	308:348	the main immunologically active component of APSs	308:356	Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs.
35281923	6	60	dep	transform	824:832	arg1	infrared					834:841	infrared	834:841	transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis	824:916	The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC-MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis.
35281923	5	61	theme	gel	682:684	arg1	column					701:706	a polyacrylamide gel chromatography column	665:706	a polyacrylamide gel chromatography column	665:706	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	8	62	from	rich	1107:1110	arg1	branches					1143:1150	C2 (25.34%) and C6 (34.54%) branches	1115:1150	C2 (25.34%) and C6 (34.54%) branches	1115:1150	The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches.
35281923	7	63	theme	cell	959:962	arg1	tests					964:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	7	64	used	used	975:978	arg2	tests					964:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests	919:968	Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products.
35281923	1	65	theme	biological	227:236	arg1	activities					238:247	biological activities	227:247	biological activities	227:247	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
35281923	0	66	theme	Components	58:67	arg1	Structure					25:33	Structure	25:33	Structure	25:33	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	0	66	theme	Components	58:67	arg1	Activity					46:53	Immune Activity	39:53	Immune Activity	39:53	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	0	67	from	Structure	25:33	arg1	APS-II					101:106	the Active Polysaccharides APS-II	74:106	the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase	74:164	Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase.
35281923	10	68	from	level	1390:1394	arg1	structure					1326:1334	the structure	1322:1334	the structure of APS-Ⅱ	1322:1343	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	10	68	from	level	1390:1394	arg1	activity					1360:1367	the immune activity	1349:1367	the immune activity from the degradation level of polysaccharides	1349:1413	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	10	69	theme	APSs	1499:1502	arg1	control					1464:1470	quality control	1456:1470	quality control	1456:1470	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	10	69	theme	APSs	1499:1502	arg1	development					1484:1494	product development	1476:1494	product development	1476:1494	In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
35281923	5	70	dep	[1-3	597:600	arg1	P2					613:614	P2	613:614	P2	613:614	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	5	70	dep	[1-3	597:600	arg1	10-18					633:637	10-18	633:637	10-18	633:637	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	5	70	dep	[1-3	597:600	arg1	7-14					618:621	7-14	618:621	7-14	618:621	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	5	70	dep	[1-3	597:600	arg1	3-6					608:610	3-6	608:610	3-6	608:610	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	5	70	dep	[1-3	597:600	arg1	P3					624:625	P3	624:625	P3	624:625	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	5	70	dep	[1-3	597:600	arg1	P4					640:641	P4	640:641	P4	640:641	The degraded products with different degrees of polymerization [1-3 (P1), 3-6 (P2), 7-14 (P3), and 10-18 (P4)] were obtained using a polyacrylamide gel chromatography column.
35281923	1	71	theme	activities	238:247	arg1	range					218:222	a wide range	211:222	a wide range of biological activities	211:247	Astragali Radix polysaccharides (APSs) have a wide range of biological activities.
36185644	7	0	theme	inflammatory	1590:1601	arg1	iNOS					1627:1630	iNOS	1627:1630	iNOS	1627:1630	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	0	theme	inflammatory	1590:1601	arg1	mediators					1603:1611	the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2	1586:1641	the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2	1586:1641	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	0	theme	inflammatory	1590:1601	arg1	TNF-α					1620:1624	TNF-α	1620:1624	TNF-α	1620:1624	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	0	theme	inflammatory	1590:1601	arg1	IL-1β					1613:1617	IL-1β	1613:1617	IL-1β	1613:1617	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	0	theme	inflammatory	1590:1601	arg1	COX-2					1637:1641	COX-2	1637:1641	COX-2	1637:1641	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	2	1	theme	sporoderm-removed	410:426	arg1	spores					428:433	the sporoderm-removed spores	406:433	the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely,	406:526	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	3	2	contain	has	635:637	arg1	RSGLP					629:633	RSGLP	629:633	RSGLP	629:633	We found that RSGLP has a higher extraction yield than BSGLP.
36185644	3	2	contain	has	635:637	arg2	yield					659:663	a higher extraction yield	639:663	a higher extraction yield than BSGLP	639:674	We found that RSGLP has a higher extraction yield than BSGLP.
36185644	7	3	dep	mediators	1603:1611	arg1	iNOS					1627:1630	iNOS	1627:1630	iNOS	1627:1630	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	3	dep	mediators	1603:1611	arg1	COX-2					1637:1641	COX-2	1637:1641	COX-2	1637:1641	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	3	dep	mediators	1603:1611	arg1	TNF-α					1620:1624	TNF-α	1620:1624	TNF-α	1620:1624	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	3	dep	mediators	1603:1611	arg1	IL-1β					1613:1617	IL-1β	1613:1617	IL-1β	1613:1617	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	3	dep	mediators	1603:1611	arg1	mediators					1603:1611	the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2	1586:1641	the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2	1586:1641	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	8	4	theme	cell	1797:1800	arg1	lines					1802:1806	multiple cancer cell lines	1781:1806	multiple cancer cell lines	1781:1806	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	7	5	theme	macrophage	1544:1553	arg1	RAW264.7					1555:1562	macrophage RAW264.7	1544:1562	macrophage RAW264.7	1544:1562	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	2	6	theme	sporoderm-broken	568:583	arg1	spores					585:590	the sporoderm-broken spores	564:590	the sporoderm-broken spores of G. lucidum (BSGLP)	564:612	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	2	7	theme	G.	595:596	arg1	BSGLP					607:611	BSGLP	607:611	BSGLP	607:611	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	2	7	theme	G.	595:596	arg1	lucidum					598:604	G. lucidum	595:604	G. lucidum (BSGLP)	595:612	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	0	8	theme	anticancer	90:99	arg1	activity					123:130	the anticancer and immune-regulatory activity	86:130	the anticancer and immune-regulatory activity of the water-soluble polysaccharide	86:166	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	6	9	theme	tumor	1257:1261	arg1	growth					1263:1268	HCT116 and NCI-H460 xenograft tumor growth	1227:1268	HCT116 and NCI-H460 xenograft tumor growth	1227:1268	Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP.
36185644	9	10	theme	promising	1940:1948	arg1	activity					1968:1975	their promising immune-regulatory activity	1934:1975	their promising immune-regulatory activity	1934:1975	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	2	11	theme	chemical	327:334	arg1	property					336:343	the chemical property	323:343	the chemical property	323:343	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	9	12	theme	sporoderm-removed	1839:1855	arg1	spores					1857:1862	the sporoderm-removed spores	1835:1862	the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP	1835:1893	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	9	12	theme	sporoderm-removed	1839:1855	arg1	agents					1923:1928	new anticancer agents	1908:1928	new anticancer agents for their promising immune-regulatory activity	1908:1975	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	5	13	dep	liver	1082:1086	arg1	Huh-7					1099:1103	Huh-7	1099:1103	Huh-7	1099:1103	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	13	dep	liver	1082:1086	arg1	HepG2					1089:1093	HepG2	1089:1093	HepG2	1089:1093	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	9	14	theme	immune-regulatory	1950:1966	arg1	activity					1968:1975	their promising immune-regulatory activity	1934:1975	their promising immune-regulatory activity	1934:1975	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	0	15	from	spores	49:54	arg1	sporoderm					13:21	the sporoderm	9:21	the sporoderm from the sporoderm-broken spores of Ganoderma lucidum	9:75	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	6	16	theme	xenograft	1247:1255	arg1	growth					1263:1268	HCT116 and NCI-H460 xenograft tumor growth	1227:1268	HCT116 and NCI-H460 xenograft tumor growth	1227:1268	Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP.
36185644	5	17	theme	flow	875:878	arg1	cytometry					880:888	flow cytometry	875:888	flow cytometry	875:888	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	4	18	theme	different	809:817	arg1	ratio					819:823	a different ratio	807:823	a different ratio of monosaccharide composition	807:853	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	2	19	theme	medicinal	442:450	arg1	RSGLP					480:484	RSGLP	480:484	RSGLP	480:484	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	2	19	theme	medicinal	442:450	arg1	lucidum					471:477	the medicinal mushroom Ganoderma lucidum	438:477	the medicinal mushroom Ganoderma lucidum (RSGLP)	438:485	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	2	20	theme	Ganoderma	461:469	arg1	RSGLP					480:484	RSGLP	480:484	RSGLP	480:484	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	2	20	theme	Ganoderma	461:469	arg1	lucidum					471:477	the medicinal mushroom Ganoderma lucidum	438:477	the medicinal mushroom Ganoderma lucidum (RSGLP)	438:485	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	0	21	theme	immune-regulatory	105:121	arg1	activity					123:130	the anticancer and immune-regulatory activity	86:130	the anticancer and immune-regulatory activity of the water-soluble polysaccharide	86:166	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	4	22	theme	composition	843:853	arg1	ratio					819:823	a different ratio	807:823	a different ratio of monosaccharide composition	807:853	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	4	22	theme	composition	843:853	arg1	weight					796:801	a higher molecular weight	777:801	a higher molecular weight	777:801	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	1	23	link	Plant-derived	169:181	arg1	polysaccharides					183:197	Plant-derived polysaccharides	169:197	Plant-derived polysaccharides	169:197	Plant-derived polysaccharides have demonstrated promising anti-cancer effects via immune-regulatory activity.
36185644	5	24	theme	cell	1033:1036	arg1	lines					1038:1042	8 cancer cell lines	1024:1042	8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549)	1024:1173	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	1	25	theme	promising	217:225	arg1	effects					239:245	promising anti-cancer effects	217:245	promising anti-cancer effects	217:245	Plant-derived polysaccharides have demonstrated promising anti-cancer effects via immune-regulatory activity.
36185644	5	26	dep	cancers	1147:1153	arg1	A549					1169:1172	A549	1169:1172	A549	1169:1172	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	26	dep	cancers	1147:1153	arg1	NCI-H460					1156:1163	NCI-H460	1156:1163	NCI-H460	1156:1163	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	26	dep	cancers	1147:1153	arg1	cancers					1147:1153	lung cancers	1142:1153	lung cancers (NCI-H460 and A549)	1142:1173	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	8	27	theme	BSGLP	1769:1773	arg1	properties					1701:1710	the chemical properties	1688:1710	the chemical properties	1688:1710	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	8	27	theme	BSGLP	1769:1773	arg1	effects					1748:1754	immune-regulatory effects	1730:1754	immune-regulatory effects	1730:1754	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	9	28	theme	G.	1867:1868	arg1	RSGL					1879:1882	RSGL	1879:1882	RSGL	1879:1882	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	9	28	theme	G.	1867:1868	arg1	lucidum					1870:1876	G. lucidum	1867:1876	G. lucidum (RSGL)	1867:1883	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	1	29	theme	anti-cancer	227:237	arg1	effects					239:245	promising anti-cancer effects	217:245	promising anti-cancer effects	217:245	Plant-derived polysaccharides have demonstrated promising anti-cancer effects via immune-regulatory activity.
36185644	6	30	theme	nude	1326:1329	arg1	mice					1331:1334	nude mice	1326:1334	nude mice	1326:1334	Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP.
36185644	8	31	theme	cancer	1790:1795	arg1	lines					1802:1806	multiple cancer cell lines	1781:1806	multiple cancer cell lines	1781:1806	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	2	32	theme	anticancer	353:362	arg1	effects					364:370	the anticancer effects	349:370	the anticancer effects of polysaccharides	349:389	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	9	33	theme	lucidum	1870:1876	arg1	spores					1857:1862	the sporoderm-removed spores	1835:1862	the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP	1835:1893	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	9	33	theme	lucidum	1870:1876	arg1	agents					1923:1928	new anticancer agents	1908:1928	new anticancer agents for their promising immune-regulatory activity	1908:1975	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	3	34	theme	extraction	648:657	arg1	yield					659:663	a higher extraction yield	639:663	a higher extraction yield than BSGLP	639:674	We found that RSGLP has a higher extraction yield than BSGLP.
36185644	8	35	theme	RSGLP	1759:1763	arg1	properties					1701:1710	the chemical properties	1688:1710	the chemical properties	1688:1710	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	8	35	theme	RSGLP	1759:1763	arg1	effects					1748:1754	immune-regulatory effects	1730:1754	immune-regulatory effects	1730:1754	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	4	36	theme	molecular	786:794	arg1	weight					796:801	a higher molecular weight	777:801	a higher molecular weight	777:801	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	8	37	theme	first	1665:1669	arg1	study					1671:1675	the first study	1661:1675	the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines	1661:1806	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	8	37	theme	first	1665:1669	arg1	This					1653:1656	This	1653:1656	This	1653:1656	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	4	38	theme	HPGPC	677:681	arg1	results					693:699	HPGPC and GC-MS results	677:699	HPGPC and GC-MS results	677:699	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	5	39	theme	MTT	867:869	arg1	results					890:896	MTT and flow cytometry results	867:896	MTT and flow cytometry results	867:896	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	4	40	contain	had	773:775	arg2	ratio					819:823	a different ratio	807:823	a different ratio of monosaccharide composition	807:853	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	4	40	contain	had	773:775	arg2	weight					796:801	a higher molecular weight	777:801	a higher molecular weight	777:801	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	4	40	contain	had	773:775	arg1	RSGLP					767:771	RSGLP	767:771	RSGLP	767:771	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	5	41	theme	cancer	1026:1031	arg1	lines					1038:1042	8 cancer cell lines	1024:1042	8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549)	1024:1173	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	4	42	theme	higher	779:784	arg1	weight					796:801	a higher molecular weight	777:801	a higher molecular weight	777:801	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	1	43	theme	immune-regulatory	251:267	arg1	activity					269:276	immune-regulatory activity	251:276	immune-regulatory activity	251:276	Plant-derived polysaccharides have demonstrated promising anti-cancer effects via immune-regulatory activity.
36185644	0	44	theme	sporoderm-broken	32:47	arg1	spores					49:54	the sporoderm-broken spores	28:54	the sporoderm-broken spores of Ganoderma lucidum	28:75	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	7	45	theme	mediators	1603:1611	arg1	activation					1530:1539	the activation	1526:1539	the activation of macrophage RAW264.7	1526:1562	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	45	theme	mediators	1603:1611	arg1	expression					1572:1581	the expression	1568:1581	the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2	1568:1641	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	0	46	theme	polysaccharide	153:166	arg1	activity					123:130	the anticancer and immune-regulatory activity	86:130	the anticancer and immune-regulatory activity of the water-soluble polysaccharide	86:166	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	5	47	theme	cytometry	880:888	arg1	results					890:896	MTT and flow cytometry results	867:896	MTT and flow cytometry results	867:896	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	48	from	apoptosis	1000:1008	arg1	lines					1038:1042	8 cancer cell lines	1024:1042	8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549)	1024:1173	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	4	49	theme	GC-MS	687:691	arg1	results					693:699	HPGPC and GC-MS results	677:699	HPGPC and GC-MS results	677:699	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	5	50	theme	lung	1142:1145	arg1	A549					1169:1172	A549	1169:1172	A549	1169:1172	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	50	theme	lung	1142:1145	arg1	NCI-H460					1156:1163	NCI-H460	1156:1163	NCI-H460	1156:1163	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	50	theme	lung	1142:1145	arg1	cancers					1147:1153	lung cancers	1142:1153	lung cancers (NCI-H460 and A549)	1142:1173	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	0	51	theme	water-soluble	139:151	arg1	polysaccharide					153:166	the water-soluble polysaccharide	135:166	the water-soluble polysaccharide	135:166	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	7	52	theme	nude	1497:1500	arg1	mice					1502:1505	nude mice	1497:1505	nude mice	1497:1505	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	3	53	theme	higher	641:646	arg1	yield					659:663	a higher extraction yield	639:663	a higher extraction yield than BSGLP	639:674	We found that RSGLP has a higher extraction yield than BSGLP.
36185644	8	54	theme	chemical	1692:1699	arg1	properties					1701:1710	the chemical properties	1688:1710	the chemical properties	1688:1710	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	2	55	theme	lucidum	471:477	arg1	spores					428:433	the sporoderm-removed spores	406:433	the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely,	406:526	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	7	56	from	level	1456:1460	arg1	mice					1502:1505	nude mice	1497:1505	nude mice	1497:1505	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	9	57	theme	RSGLP	1889:1893	arg1	spores					1857:1862	the sporoderm-removed spores	1835:1862	the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP	1835:1893	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	9	57	theme	RSGLP	1889:1893	arg1	agents					1923:1928	new anticancer agents	1908:1928	new anticancer agents for their promising immune-regulatory activity	1908:1975	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	2	58	theme	lucidum	598:604	arg1	spores					585:590	the sporoderm-broken spores	564:590	the sporoderm-broken spores of G. lucidum (BSGLP)	564:612	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	5	59	dep	breast	1107:1112	arg1	MDA-MB-231					1115:1124	MDA-MB-231	1115:1124	MDA-MB-231	1115:1124	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	59	dep	breast	1107:1112	arg1	MCF-7					1130:1134	MCF-7	1130:1134	MCF-7	1130:1134	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	7	60	theme	cytokines	1484:1492	arg1	level					1456:1460	the level	1452:1460	the level of serum inflammatory cytokines in nude mice	1452:1505	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	5	61	theme	cell	972:975	arg1	viability					977:985	cell viability	972:985	cell viability	972:985	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	4	62	theme	monosaccharide	828:841	arg1	composition					843:853	monosaccharide composition	828:853	monosaccharide composition	828:853	HPGPC and GC-MS results revealed that both RSGLP and BSGLP are heteropolysaccharides, but RSGLP had a higher molecular weight and a different ratio of monosaccharide composition than BSGLP.
36185644	7	63	dep	potent	1431:1436	arg1	inhibiting					1515:1524	inhibiting	1515:1524	inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro	1515:1650	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	63	dep	potent	1431:1436	arg1	inhibiting					1441:1450	inhibiting	1441:1450	inhibiting the level of serum inflammatory cytokines in nude mice	1441:1505	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	6	64	from	BSGLP	1317:1321	arg1	mice					1331:1334	nude mice	1326:1334	nude mice	1326:1334	Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP.
36185644	2	65	theme	polysaccharides	375:389	arg1	effects					364:370	the anticancer effects	349:370	the anticancer effects of polysaccharides	349:389	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	9	66	theme	anticancer	1912:1921	arg1	spores					1857:1862	the sporoderm-removed spores	1835:1862	the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP	1835:1893	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	9	66	theme	anticancer	1912:1921	arg1	agents					1923:1928	new anticancer agents	1908:1928	new anticancer agents for their promising immune-regulatory activity	1908:1975	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	7	67	theme	RAW264.7	1555:1562	arg1	activation					1530:1539	the activation	1526:1539	the activation of macrophage RAW264.7	1526:1562	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	7	67	theme	RAW264.7	1555:1562	arg1	expression					1572:1581	the expression	1568:1581	the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2	1568:1641	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	6	68	theme	tumor-induced	1285:1297	arg1	splenomegaly					1299:1310	tumor-induced splenomegaly	1285:1310	tumor-induced splenomegaly	1285:1310	Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP.
36185644	7	69	theme	serum	1465:1469	arg1	cytokines					1484:1492	serum inflammatory cytokines	1465:1492	serum inflammatory cytokines	1465:1492	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	8	70	theme	multiple	1781:1788	arg1	lines					1802:1806	multiple cancer cell lines	1781:1806	multiple cancer cell lines	1781:1806	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	9	71	theme	new	1908:1910	arg1	spores					1857:1862	the sporoderm-removed spores	1835:1862	the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP	1835:1893	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	9	71	theme	new	1908:1910	arg1	agents					1923:1928	new anticancer agents	1908:1928	new anticancer agents for their promising immune-regulatory activity	1908:1975	Our results revealed that the sporoderm-removed spores of G. lucidum (RSGL) and RSGLP may serve as new anticancer agents for their promising immune-regulatory activity.
36185644	5	72	theme	higher	937:942	arg1	dose-efficacy					944:956	much higher dose-efficacy	932:956	much higher dose-efficacy	932:956	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	0	73	theme	lucidum	69:75	arg1	spores					49:54	the sporoderm-broken spores	28:54	the sporoderm-broken spores of Ganoderma lucidum	28:75	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	7	74	theme	inflammatory	1471:1482	arg1	cytokines					1484:1492	serum inflammatory cytokines	1465:1492	serum inflammatory cytokines	1465:1492	Next, we found that RSGLP is more potent in inhibiting the level of serum inflammatory cytokines in nude mice, and in inhibiting the activation of macrophage RAW264.7 and the expression of the inflammatory mediators IL-1β, TNF-α, iNOS, and COX-2 in vitro.
36185644	2	75	theme	study	302:306	arg1	aim					283:285	The aim	279:285	The aim of the current study	279:306	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	5	76	dep	colon	1057:1061	arg1	HT29					1075:1078	HT29	1075:1078	HT29	1075:1078	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	5	76	dep	colon	1057:1061	arg1	HCT116					1064:1069	HCT116	1064:1069	HCT116	1064:1069	MTT and flow cytometry results demonstrated that RSGLP exhibited much higher dose-efficacy in inhibiting cell viability and inducing apoptosis than BSGLP in 8 cancer cell lines representing colon (HCT116 and HT29), liver (HepG2 and Huh-7), breast (MDA-MB-231 and MCF-7), and lung cancers (NCI-H460 and A549).
36185644	6	77	theme	RSGLP	1390:1394	arg1	immunity					1378:1385	immunity	1378:1385	immunity of RSGLP	1378:1394	Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP.
36185644	2	78	theme	mushroom	452:459	arg1	RSGLP					480:484	RSGLP	480:484	RSGLP	480:484	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	2	78	theme	mushroom	452:459	arg1	lucidum					471:477	the medicinal mushroom Ganoderma lucidum	438:477	the medicinal mushroom Ganoderma lucidum (RSGLP)	438:485	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	0	79	theme	Ganoderma	59:67	arg1	lucidum					69:75	Ganoderma lucidum	59:75	Ganoderma lucidum	59:75	Removing the sporoderm from the sporoderm-broken spores of Ganoderma lucidum improves the anticancer and immune-regulatory activity of the water-soluble polysaccharide.
36185644	2	80	theme	current	294:300	arg1	study					302:306	the current study	290:306	the current study	290:306	The aim of the current study was to compare the chemical property and the anticancer effects of polysaccharides extracted from the sporoderm-removed spores of the medicinal mushroom Ganoderma lucidum (RSGLP), which removed the sporoderm completely, with polysaccharides extracted from the sporoderm-broken spores of G. lucidum (BSGLP).
36185644	8	81	theme	immune-regulatory	1730:1746	arg1	effects					1748:1754	immune-regulatory effects	1730:1754	immune-regulatory effects	1730:1754	This is the first study to compare the chemical properties, anti-cancer, and immune-regulatory effects of RSGLP and BSGLP using multiple cancer cell lines.
36185644	1	82	theme	Plant-derived	169:181	arg1	polysaccharides					183:197	Plant-derived polysaccharides	169:197	Plant-derived polysaccharides	169:197	Plant-derived polysaccharides have demonstrated promising anti-cancer effects via immune-regulatory activity.
36185644	6	83	theme	better	1350:1355	arg1	effect					1357:1362	a better effect	1348:1362	a better effect on regulating immunity of RSGLP	1348:1394	Furthermore, RSGLP is more effective in inhibiting HCT116 and NCI-H460 xenograft tumor growth and inhibiting tumor-induced splenomegaly than BSGLP in nude mice, suggesting a better effect on regulating immunity of RSGLP.
37249303	1	0	theme	acid-labile	190:200	arg1	oximes					202:207	acid-labile oximes	190:207	acid-labile oximes	190:207	A highly efficient, palladium-catalyzed glycosylation between 3,4-O-carbonate glycals and acid-labile oximes is disclosed.
37249303	3	1	theme	bioactive	582:590	arg1	molecules					592:600	bioactive molecules	582:600	bioactive molecules	582:600	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	4	2	link	N-O-linked	685:694	arg1	glycosides					696:705	valuable N-O-linked glycosides	676:705	valuable N-O-linked glycosides	676:705	Overall, our strategy provides an efficient toolkit for facile access to valuable N-O-linked glycosides.
37249303	3	3	theme	products	534:541	arg1	transformations					501:515	site-selective transformations	486:515	site-selective transformations of glycosylation products	486:541	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	4	4	theme	facile	659:664	arg1	access					666:671	facile access	659:671	facile access to valuable N-O-linked glycosides	659:705	Overall, our strategy provides an efficient toolkit for facile access to valuable N-O-linked glycosides.
37249303	3	5	theme	method	451:456	arg1	power					437:441	The power	433:441	The power of this method	433:456	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	2	6	theme	easy	306:309	arg1	scalability					311:321	easy scalability	306:321	easy scalability	306:321	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	0	7	theme	Palladium-Catalyzed	15:33	arg1	Glycosylation					42:54	Stereospecific Palladium-Catalyzed Direct Glycosylation	0:54	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.	0:98	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.
37249303	2	8	theme	excellent	354:362	arg1	yields					364:369	excellent yields	354:369	excellent yields with exclusive β-selectivity and retention of Z/E geometries	354:430	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	0	9	theme	Stereospecific	0:13	arg1	Glycosylation					42:54	Stereospecific Palladium-Catalyzed Direct Glycosylation	0:54	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.	0:98	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.
37249303	2	10	theme	geometries	421:430	arg1	retention					404:412	retention	404:412	retention	404:412	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	2	10	theme	geometries	421:430	arg1	β-selectivity					386:398	exclusive β-selectivity	376:398	exclusive β-selectivity	376:398	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	1	11	theme	efficient	109:117	arg1	glycosylation					140:152	A highly efficient, palladium-catalyzed glycosylation	100:152	A highly efficient, palladium-catalyzed glycosylation between 3,4-O-carbonate glycals and acid-labile oximes	100:207	A highly efficient, palladium-catalyzed glycosylation between 3,4-O-carbonate glycals and acid-labile oximes is disclosed.
37249303	3	12	theme	transformations	501:515	arg1	set					479:481	a set	477:481	a set of site-selective transformations of glycosylation products	477:541	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	3	12	theme	transformations	501:515	arg1	transformations					501:515	site-selective transformations	486:515	site-selective transformations of glycosylation products	486:541	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	3	12	theme	transformations	501:515	arg1	glycodiversification					558:577	late-stage glycodiversification	547:577	late-stage glycodiversification of bioactive molecules	547:600	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	3	12	theme	transformations	501:515	arg1	molecules					592:600	bioactive molecules	582:600	bioactive molecules	582:600	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	0	13	theme	Direct	35:40	arg1	Glycosylation					42:54	Stereospecific Palladium-Catalyzed Direct Glycosylation	0:54	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.	0:98	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.
37249303	4	14	theme	efficient	637:645	arg1	toolkit					647:653	an efficient toolkit	634:653	an efficient toolkit for facile access to valuable N-O-linked glycosides	634:705	Overall, our strategy provides an efficient toolkit for facile access to valuable N-O-linked glycosides.
37249303	2	15	theme	glycosyl	335:342	arg1	oximes					344:349	glycosyl oximes	335:349	glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries	335:430	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	1	16	dep	efficient	109:117	arg1	palladium-catalyzed					120:138	palladium-catalyzed	120:138	palladium-catalyzed	120:138	A highly efficient, palladium-catalyzed glycosylation between 3,4-O-carbonate glycals and acid-labile oximes is disclosed.
37249303	0	17	theme	Oximes	59:64	arg1	Glycosylation					42:54	Stereospecific Palladium-Catalyzed Direct Glycosylation	0:54	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.	0:98	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.
37249303	3	18	theme	late-stage	547:556	arg1	glycodiversification					558:577	late-stage glycodiversification	547:577	late-stage glycodiversification of bioactive molecules	547:600	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	2	19	with	yields	364:369	arg1	retention					404:412	retention	404:412	retention	404:412	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	2	19	with	yields	364:369	arg1	β-selectivity					386:398	exclusive β-selectivity	376:398	exclusive β-selectivity	376:398	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	2	20	theme	functional	274:283	arg1	tolerance					291:299	high functional group tolerance	269:299	high functional group tolerance	269:299	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	2	21	theme	high	269:272	arg1	tolerance					291:299	high functional group tolerance	269:299	high functional group tolerance	269:299	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	3	22	theme	site-selective	486:499	arg1	transformations					501:515	site-selective transformations	486:515	site-selective transformations of glycosylation products	486:541	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	2	23	theme	substrate	252:260	arg1	scope					262:266	broad substrate scope	246:266	broad substrate scope	246:266	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	0	24	gly	Glycosylation	42:54	arg1	Oximes					59:64	Oximes	59:64	Oximes	59:64	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.
37249303	2	25	theme	exclusive	376:384	arg1	β-selectivity					386:398	exclusive β-selectivity	376:398	exclusive β-selectivity	376:398	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	2	26	theme	broad	246:250	arg1	scope					262:266	broad substrate scope	246:266	broad substrate scope	246:266	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	0	27	dep	Glycosylation	42:54	arg1	Access					67:72	Access	67:72	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.	0:98	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.
37249303	3	28	theme	glycosylation	520:532	arg1	products					534:541	glycosylation products	520:541	glycosylation products	520:541	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	4	29	theme	valuable	676:683	arg1	glycosides					696:705	valuable N-O-linked glycosides	676:705	valuable N-O-linked glycosides	676:705	Overall, our strategy provides an efficient toolkit for facile access to valuable N-O-linked glycosides.
37249303	1	30	theme	3,4-O-carbonate	162:176	arg1	glycals					178:184	3,4-O-carbonate glycals	162:184	3,4-O-carbonate glycals	162:184	A highly efficient, palladium-catalyzed glycosylation between 3,4-O-carbonate glycals and acid-labile oximes is disclosed.
37249303	0	31	theme	N-O-Linked	77:86	arg1	Glycosides					88:97	N-O-Linked Glycosides	77:97	N-O-Linked Glycosides	77:97	Stereospecific Palladium-Catalyzed Direct Glycosylation of Oximes: Access to N-O-Linked Glycosides.
37249303	2	32	theme	Z/E	417:419	arg1	geometries					421:430	Z/E geometries	417:430	Z/E geometries	417:430	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	2	33	theme	group	285:289	arg1	tolerance					291:299	high functional group tolerance	269:299	high functional group tolerance	269:299	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	3	34	theme	molecules	592:600	arg1	set					479:481	a set	477:481	a set of site-selective transformations of glycosylation products	477:541	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	3	34	theme	molecules	592:600	arg1	transformations					501:515	site-selective transformations	486:515	site-selective transformations of glycosylation products	486:541	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	3	34	theme	molecules	592:600	arg1	glycodiversification					558:577	late-stage glycodiversification	547:577	late-stage glycodiversification of bioactive molecules	547:600	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	3	34	theme	molecules	592:600	arg1	molecules					592:600	bioactive molecules	582:600	bioactive molecules	582:600	The power of this method is demonstrated by a set of site-selective transformations of glycosylation products and late-stage glycodiversification of bioactive molecules.
37249303	2	35	from	oximes	344:349	arg1	yields					364:369	excellent yields	354:369	excellent yields with exclusive β-selectivity and retention of Z/E geometries	354:430	This approach features broad substrate scope, high functional group tolerance, and easy scalability, delivering glycosyl oximes in excellent yields with exclusive β-selectivity and retention of Z/E geometries.
37249303	4	36	theme	N-O-linked	685:694	arg1	glycosides					696:705	valuable N-O-linked glycosides	676:705	valuable N-O-linked glycosides	676:705	Overall, our strategy provides an efficient toolkit for facile access to valuable N-O-linked glycosides.
35366452	0	0	theme	inhibitory	74:83	arg1	effect					85:90	inhibitory effect	74:90	inhibitory effect of phosphate	74:103	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	1	1	theme	lake	293:296	arg1	state					282:286	the trophic state	270:286	the trophic state of a lake	270:296	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	1	2	theme	Humic	148:152	arg1	HA					160:161	HA	160:161	HA	160:161	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	1	2	theme	Humic	148:152	arg1	acid					154:157	Humic acid	148:157	Humic acid (HA)	148:162	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	1	3	theme	biogeochemical	216:229	arg1	cycle					231:235	the biogeochemical cycle	212:235	the biogeochemical cycle of carbon and nutrients	212:259	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	8	4	theme	feedback	1458:1465	arg1	loop					1467:1470	a positive feedback loop	1447:1470	a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication	1447:1542	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	8	5	dep	eutrophic	1309:1317	arg1	algae-dominated					1320:1334	algae-dominated	1320:1334	algae-dominated	1320:1334	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	2	6	theme	adsorption	303:312	arg1	behavior					314:321	The adsorption behavior	299:321	The adsorption behavior of HAs to sediments in the absence and presence of phosphate	299:382	The adsorption behavior of HAs to sediments in the absence and presence of phosphate was investigated in this study.
35366452	3	7	from	sediments	476:484	arg1	HA					549:550	a reference HA	537:550	a reference HA (RHA) with terrestrial sources	537:581	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	7	from	sediments	476:484	arg1	AHA					446:448	AHA	446:448	AHA from algae-dominated lake sediments	446:484	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	7	from	sediments	476:484	arg1	MHA					487:489	MHA	487:489	MHA from macrophyte-dominated lake sediments	487:530	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	7	from	sediments	476:484	arg1	RHA					553:555	RHA	553:555	RHA	553:555	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	6	8	theme	organic	1037:1043	arg1	compounds					1045:1053	organic compounds	1037:1053	organic compounds	1037:1053	Our findings show that the functional groups of organic compounds control not only their fractionation and burial but also their ability to replace phosphate in sediments.
35366452	8	9	theme	algae-derived	1354:1366	arg1	compounds					1376:1384	algae-derived organic compounds	1354:1384	algae-derived organic compounds	1354:1384	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	3	10	with	AHA	446:448	arg1	sources					575:581	terrestrial sources	563:581	terrestrial sources	563:581	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	8	11	theme	organic	1368:1374	arg1	compounds					1376:1384	algae-derived organic compounds	1354:1384	algae-derived organic compounds	1354:1384	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	8	12	theme	lake	1524:1527	arg1	eutrophication					1529:1542	lake eutrophication	1524:1542	lake eutrophication	1524:1542	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	8	13	theme	eutrophic	1309:1317	arg1	state					1336:1340	more eutrophic, algae-dominated state	1304:1340	more eutrophic, algae-dominated state	1304:1340	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	8	14	from	sediments	1428:1436	arg1	release					1402:1408	the release	1398:1408	the release of phosphate from sediments	1398:1436	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	5	15	theme	stronger	838:845	arg1	effect					858:863	a stronger inhibitory effect	836:863	a stronger inhibitory effect on MHA adsorption	836:881	Phosphate showed a stronger inhibitory effect on MHA adsorption than on AHA adsorption, suggesting that AHA can more effectively replace phosphate adsorbed to sediments.
35366452	1	16	theme	carbon	240:245	arg1	cycle					231:235	the biogeochemical cycle	212:235	the biogeochemical cycle of carbon and nutrients	212:259	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	3	17	theme	algae-dominated	455:469	arg1	sediments					476:484	algae-dominated lake sediments	455:484	algae-dominated lake sediments	455:484	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	0	18	theme	phosphate	95:103	arg1	fractionation					59:71	Compositional fractionation	45:71	Compositional fractionation	45:71	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	0	18	theme	phosphate	95:103	arg1	effect					85:90	inhibitory effect	74:90	inhibitory effect of phosphate	74:103	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	0	18	theme	phosphate	95:103	arg1	implications					110:121	implications	110:121	implications for lake eutrophication	110:145	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	5	19	theme	inhibitory	847:856	arg1	effect					858:863	a stronger inhibitory effect	836:863	a stronger inhibitory effect on MHA adsorption	836:881	Phosphate showed a stronger inhibitory effect on MHA adsorption than on AHA adsorption, suggesting that AHA can more effectively replace phosphate adsorbed to sediments.
35366452	3	20	theme	lake	471:474	arg1	sediments					476:484	algae-dominated lake sediments	455:484	algae-dominated lake sediments	455:484	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	6	21	theme	functional	1016:1025	arg1	compounds					1045:1053	organic compounds	1037:1053	organic compounds	1037:1053	Our findings show that the functional groups of organic compounds control not only their fractionation and burial but also their ability to replace phosphate in sediments.
35366452	6	21	theme	functional	1016:1025	arg1	groups					1027:1032	the functional groups	1012:1032	the functional groups of organic compounds	1012:1053	Our findings show that the functional groups of organic compounds control not only their fractionation and burial but also their ability to replace phosphate in sediments.
35366452	3	22	with	MHA	487:489	arg1	sources					575:581	terrestrial sources	563:581	terrestrial sources	563:581	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	23	theme	macrophyte-dominated	496:515	arg1	sediments					522:530	macrophyte-dominated lake sediments	496:530	macrophyte-dominated lake sediments	496:530	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	5	24	from	effect	858:863	arg1	adsorption					872:881	MHA adsorption	868:881	MHA adsorption	868:881	Phosphate showed a stronger inhibitory effect on MHA adsorption than on AHA adsorption, suggesting that AHA can more effectively replace phosphate adsorbed to sediments.
35366452	3	25	theme	lake	517:520	arg1	sediments					522:530	macrophyte-dominated lake sediments	496:530	macrophyte-dominated lake sediments	496:530	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	26	from	sediments	522:530	arg1	HA					549:550	a reference HA	537:550	a reference HA (RHA) with terrestrial sources	537:581	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	26	from	sediments	522:530	arg1	AHA					446:448	AHA	446:448	AHA from algae-dominated lake sediments	446:484	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	26	from	sediments	522:530	arg1	MHA					487:489	MHA	487:489	MHA from macrophyte-dominated lake sediments	487:530	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	26	from	sediments	522:530	arg1	RHA					553:555	RHA	553:555	RHA	553:555	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	4	27	theme	adsorption	588:597	arg1	highest					630:636	highest	630:636	highest	630:636	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	4	27	theme	adsorption	588:597	arg1	capacity					599:606	The adsorption capacity	584:606	The adsorption capacity of lake sediments	584:624	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	0	28	theme	acids	20:24	arg1	Adsorption					0:9	Adsorption	0:9	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.	0:146	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	1	29	theme	nutrients	251:259	arg1	cycle					231:235	the biogeochemical cycle	212:235	the biogeochemical cycle of carbon and nutrients	212:259	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	5	30	theme	AHA	891:893	arg1	adsorption					895:904	AHA adsorption	891:904	AHA adsorption	891:904	Phosphate showed a stronger inhibitory effect on MHA adsorption than on AHA adsorption, suggesting that AHA can more effectively replace phosphate adsorbed to sediments.
35366452	1	31	theme	phosphate	168:176	arg1	interactions					178:189	phosphate interactions	168:189	phosphate interactions	168:189	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	2	32	theme	phosphate	374:382	arg1	presence					362:369	presence	362:369	presence	362:369	The adsorption behavior of HAs to sediments in the absence and presence of phosphate was investigated in this study.
35366452	2	32	theme	phosphate	374:382	arg1	absence					350:356	absence	350:356	absence	350:356	The adsorption behavior of HAs to sediments in the absence and presence of phosphate was investigated in this study.
35366452	3	33	theme	terrestrial	563:573	arg1	sources					575:581	terrestrial sources	563:581	terrestrial sources	563:581	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	4	34	theme	sediments	616:624	arg1	highest					630:636	highest	630:636	highest	630:636	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	4	34	theme	sediments	616:624	arg1	capacity					599:606	The adsorption capacity	584:606	The adsorption capacity of lake sediments	584:624	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	8	35	link	algae-derived	1354:1366	arg1	compounds					1376:1384	algae-derived organic compounds	1354:1384	algae-derived organic compounds	1354:1384	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	4	36	theme	lake	611:614	arg1	sediments					616:624	lake sediments	611:624	lake sediments	611:624	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	0	37	theme	lake	29:32	arg1	sediments					34:42	lake sediments	29:42	lake sediments	29:42	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	8	38	theme	lakes	1255:1259	arg1	shift					1261:1265	lakes shift	1255:1265	lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state	1255:1340	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	0	39	theme	lake	127:130	arg1	eutrophication					132:145	lake eutrophication	127:145	lake eutrophication	127:145	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	7	40	theme	eutrophication	1227:1240	arg1	effect					1212:1217	the legacy effect	1201:1217	the legacy effect of lake eutrophication	1201:1240	We propose a novel mechanism to explain the legacy effect of lake eutrophication.
35366452	0	41	theme	humic	14:18	arg1	acids					20:24	humic acids	14:24	humic acids	14:24	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	2	42	dep	absence	350:356	arg1	the					346:348	the	346:348	the	346:348	The adsorption behavior of HAs to sediments in the absence and presence of phosphate was investigated in this study.
35366452	2	43	from	sediments	333:341	arg1	presence					362:369	presence	362:369	presence	362:369	The adsorption behavior of HAs to sediments in the absence and presence of phosphate was investigated in this study.
35366452	2	43	from	sediments	333:341	arg1	absence					350:356	absence	350:356	absence	350:356	The adsorption behavior of HAs to sediments in the absence and presence of phosphate was investigated in this study.
35366452	1	44	theme	trophic	274:280	arg1	state					282:286	the trophic state	270:286	the trophic state of a lake	270:296	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	3	45	with	HA	549:550	arg1	sources					575:581	terrestrial sources	563:581	terrestrial sources	563:581	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	6	46	dep	control	1055:1061	arg1	only					1067:1070	only	1067:1070	only	1067:1070	Our findings show that the functional groups of organic compounds control not only their fractionation and burial but also their ability to replace phosphate in sediments.
35366452	8	47	theme	internal	1486:1493	arg1	loading					1506:1512	internal phosphorus loading	1486:1512	internal phosphorus loading	1486:1512	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	3	48	theme	HAs	431:433	arg1	types					422:426	Three types	416:426	Three types of HAs	416:433	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	8	49	theme	positive	1449:1456	arg1	loop					1467:1470	a positive feedback loop	1447:1470	a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication	1447:1542	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	7	50	theme	lake	1222:1225	arg1	eutrophication					1227:1240	lake eutrophication	1222:1240	lake eutrophication	1222:1240	We propose a novel mechanism to explain the legacy effect of lake eutrophication.
35366452	8	51	from	state	1295:1299	arg1	shift					1261:1265	lakes shift	1255:1265	lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state	1255:1340	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	8	52	theme	phosphate	1413:1421	arg1	release					1402:1408	the release	1398:1408	the release of phosphate from sediments	1398:1436	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	4	53	theme	carboxyl-containing	697:715	arg1	molecules					717:725	more carboxyl-containing molecules	692:725	more carboxyl-containing molecules	692:725	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	4	54	theme	proteinaceous	728:740	arg1	compounds					742:750	proteinaceous compounds	728:750	proteinaceous compounds	728:750	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	5	55	theme	MHA	868:870	arg1	adsorption					872:881	MHA adsorption	868:881	MHA adsorption	868:881	Phosphate showed a stronger inhibitory effect on MHA adsorption than on AHA adsorption, suggesting that AHA can more effectively replace phosphate adsorbed to sediments.
35366452	1	56	theme	vital	198:202	arg1	role					204:207	a vital role	196:207	a vital role	196:207	Humic acid (HA) and phosphate interactions play a vital role in the biogeochemical cycle of carbon and nutrients and thus the trophic state of a lake.
35366452	8	57	theme	macrophyte-dominated	1274:1293	arg1	state					1295:1299	a macrophyte-dominated state	1272:1299	a macrophyte-dominated state	1272:1299	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	3	58	used	used	440:443	arg2	types					422:426	Three types	416:426	Three types of HAs	416:433	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	7	59	theme	legacy	1205:1210	arg1	effect					1212:1217	the legacy effect	1201:1217	the legacy effect of lake eutrophication	1201:1240	We propose a novel mechanism to explain the legacy effect of lake eutrophication.
35366452	3	60	theme	reference	539:547	arg1	HA					549:550	a reference HA	537:550	a reference HA (RHA) with terrestrial sources	537:581	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	3	60	theme	reference	539:547	arg1	RHA					553:555	RHA	553:555	RHA	553:555	Three types of HAs were used, AHA from algae-dominated lake sediments, MHA from macrophyte-dominated lake sediments, and a reference HA (RHA) with terrestrial sources.
35366452	8	61	theme	phosphorus	1495:1504	arg1	loading					1506:1512	internal phosphorus loading	1486:1512	internal phosphorus loading	1486:1512	That is, as lakes shift from a macrophyte-dominated state to more eutrophic, algae-dominated state, increasing algae-derived organic compounds can promote the release of phosphate from sediments, forming a positive feedback loop that sustains internal phosphorus loading and hence lake eutrophication.
35366452	2	62	theme	HAs	326:328	arg1	behavior					314:321	The adsorption behavior	299:321	The adsorption behavior of HAs to sediments in the absence and presence of phosphate	299:382	The adsorption behavior of HAs to sediments in the absence and presence of phosphate was investigated in this study.
35366452	7	63	theme	novel	1174:1178	arg1	mechanism					1180:1188	a novel mechanism	1172:1188	a novel mechanism to explain the legacy effect of lake eutrophication	1172:1240	We propose a novel mechanism to explain the legacy effect of lake eutrophication.
35366452	0	64	theme	Compositional	45:57	arg1	fractionation					59:71	Compositional fractionation	45:71	Compositional fractionation	45:71	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	0	65	dep	Adsorption	0:9	arg1	implications					110:121	implications	110:121	implications for lake eutrophication	110:145	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	0	65	dep	Adsorption	0:9	arg1	effect					85:90	inhibitory effect	74:90	inhibitory effect of phosphate	74:103	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	0	65	dep	Adsorption	0:9	arg1	fractionation					59:71	Compositional fractionation	45:71	Compositional fractionation	45:71	Adsorption of humic acids to lake sediments: Compositional fractionation, inhibitory effect of phosphate, and implications for lake eutrophication.
35366452	6	66	theme	compounds	1045:1053	arg1	compounds					1045:1053	organic compounds	1037:1053	organic compounds	1037:1053	Our findings show that the functional groups of organic compounds control not only their fractionation and burial but also their ability to replace phosphate in sediments.
35366452	6	66	theme	compounds	1045:1053	arg1	groups					1027:1032	the functional groups	1012:1032	the functional groups of organic compounds	1012:1053	Our findings show that the functional groups of organic compounds control not only their fractionation and burial but also their ability to replace phosphate in sediments.
35366452	4	67	contain	contained	682:690	arg2	polysaccharides					756:770	polysaccharides	756:770	polysaccharides	756:770	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	4	67	contain	contained	682:690	arg2	compounds					742:750	proteinaceous compounds	728:750	proteinaceous compounds	728:750	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	4	67	contain	contained	682:690	arg1	AHA					678:680	AHA	678:680	AHA	678:680	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
35366452	4	67	contain	contained	682:690	arg2	molecules					717:725	more carboxyl-containing molecules	692:725	more carboxyl-containing molecules	692:725	The adsorption capacity of lake sediments was highest for AHA, which can be explained by that AHA contained more carboxyl-containing molecules, proteinaceous compounds and polysaccharides that were preferentially adsorbed by minerals.
36807552	0	0	theme	Decoction	86:94	arg1	composition					63:73	the chemical composition	50:73	the chemical composition of Sijunzi Decoction	50:94	Multiple approaches to characterize and visualize the chemical composition of Sijunzi Decoction comprehensively.
36807552	7	1	theme	substance	1409:1417	arg1	basis					1419:1423	substance basis	1409:1423	substance basis of other Chinese medicine	1409:1449	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	7	2	theme	Chinese	1434:1440	arg1	medicine					1442:1449	other Chinese medicine	1428:1449	other Chinese medicine	1428:1449	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	7	3	theme	medicine	1442:1449	arg1	basis					1419:1423	substance basis	1409:1423	substance basis of other Chinese medicine	1409:1449	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	5	4	dep	%	1030:1030	arg1	flavonoids					1038:1047	total flavonoids	1032:1047	1.177% total flavonoids	1025:1047	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	5	theme	polymerization	925:938	arg1	degree					915:920	degree	915:920	degree of polymerization 1-2	915:942	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	6	theme	inorganic	1089:1097	arg1	elements					1099:1106	inorganic elements	1089:1106	0.483% inorganic elements	1082:1106	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	3	7	from	acids	510:514	arg1	decoction					584:592	the decoction	580:592	the decoction	580:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	7	8	theme	present	1225:1231	arg1	study					1233:1237	The present study	1221:1237	The present study	1221:1237	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	5	9	dep	sugars	907:912	arg1	degree					915:920	degree	915:920	degree of polymerization 1-2	915:942	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	1	10	theme	Chinese	342:348	arg1	medicine					350:357	Chinese medicine	342:357	Chinese medicine	342:357	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	7	11	theme	constituent	1358:1368	arg1	type					1350:1353	each type	1345:1353	each type of constituent	1345:1368	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	3	12	theme	phenolic	539:546	arg1	acids					548:552	phenolic acids	539:552	phenolic acids	539:552	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	3	13	from	acids	548:552	arg1	decoction					584:592	the decoction	580:592	the decoction	580:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	5	14	theme	total	1057:1061	arg1	acids					1072:1076	total phenolic acids	1057:1076	0.546% total phenolic acids	1050:1076	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	6	15	theme	chemical	1178:1185	arg1	composition					1187:1197	the chemical composition	1174:1197	the chemical composition of Sijunzi Decoction	1174:1218	Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction.
36807552	3	16	from	saponins	517:524	arg1	decoction					584:592	the decoction	580:592	the decoction	580:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	4	17	from	ingredients	680:690	arg1	Decoction					703:711	Decoction	703:711	Decoction	703:711	Molecular network also used for visualizing the ingredients in Sijunzi Decoction, and representative components were also quantified.
36807552	5	18	theme	phenolic	1063:1070	arg1	acids					1072:1076	total phenolic acids	1057:1076	0.546% total phenolic acids	1050:1076	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	19	dep	%	974:974	arg1	precipitates					986:997	insoluble precipitates	976:997	2.427% insoluble precipitates	969:997	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	20	theme	crude	876:880	arg1	polysaccharides					882:896	crude polysaccharides	876:896	41.751% crude polysaccharides	868:896	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	2	21	theme	innovative	457:466	arg1	medicines					468:476	innovative medicines	457:476	innovative medicines	457:476	Clarifying the active substances is an effective way to develop Traditional Chinese medicine and innovative medicines.
36807552	7	22	theme	other	1428:1432	arg1	medicine					1442:1449	other Chinese medicine	1428:1449	other Chinese medicine	1428:1449	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	5	23	dep	%	951:951	arg1	saponins					959:966	total saponins	953:966	8.181% total saponins	946:966	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	24	theme	Decoction	827:835	arg1	powder					850:855	the Sijunzi Decoction freeze-dried powder	815:855	the Sijunzi Decoction freeze-dried powder	815:855	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	25	dep	%	874:874	arg1	polysaccharides					882:896	crude polysaccharides	876:896	41.751% crude polysaccharides	868:896	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	3	26	from	proteins	494:501	arg1	decoction					584:592	the decoction	580:592	the decoction	580:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	6	27	theme	Decoction	1210:1218	arg1	composition					1187:1197	the chemical composition	1174:1197	the chemical composition of Sijunzi Decoction	1174:1218	Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction.
36807552	5	28	dep	%	1005:1005	arg1	acids					1018:1022	free amino acids	1007:1022	2.154% free amino acids	1000:1022	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	0	29	theme	Multiple	0:7	arg1	approaches					9:18	Multiple approaches	0:18	Multiple approaches	0:18	Multiple approaches to characterize and visualize the chemical composition of Sijunzi Decoction comprehensively.
36807552	2	30	theme	active	375:380	arg1	substances					382:391	the active substances	371:391	the active substances	371:391	Clarifying the active substances is an effective way to develop Traditional Chinese medicine and innovative medicines.
36807552	2	31	theme	Chinese	436:442	arg1	medicine					444:451	Traditional Chinese medicine	424:451	Traditional Chinese medicine	424:451	Clarifying the active substances is an effective way to develop Traditional Chinese medicine and innovative medicines.
36807552	6	32	theme	Molecular	1109:1117	arg1	network					1119:1125	Molecular network	1109:1125	Molecular network	1109:1125	Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction.
36807552	5	33	theme	total	953:957	arg1	saponins					959:966	total saponins	953:966	8.181% total saponins	946:966	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	2	34	theme	Traditional	424:434	arg1	medicine					444:451	Traditional Chinese medicine	424:451	Traditional Chinese medicine	424:451	Clarifying the active substances is an effective way to develop Traditional Chinese medicine and innovative medicines.
36807552	3	35	theme	inorganic	558:566	arg1	elements					568:575	inorganic elements	558:575	inorganic elements in the decoction	558:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	6	36	used	used	1153:1156	arg2	analysis					1144:1151	quantitative analysis	1131:1151	quantitative analysis	1131:1151	Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction.
36807552	6	36	used	used	1153:1156	arg2	network					1119:1125	Molecular network	1109:1125	Molecular network	1109:1125	Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction.
36807552	4	37	theme	Molecular	632:640	arg1	network					642:648	Molecular network	632:648	Molecular network also used for visualizing the ingredients in Sijunzi Decoction	632:711	Molecular network also used for visualizing the ingredients in Sijunzi Decoction, and representative components were also quantified.
36807552	7	38	theme	Decoction	1296:1304	arg1	constituents					1272:1283	the constituents	1268:1283	the constituents of Sijunzi Decoction	1268:1304	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	1	39	theme	classic	283:289	arg1	it					275:276	it	275:276	it	275:276	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	1	39	theme	classic	283:289	arg1	formula					291:297	a classic formula	281:297	a classic formula for treating spleen deficiency syndrome in Chinese medicine	281:357	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	1	40	dep	Rhizoma	199:205	arg1	Melle					264:268	Melle	264:268	Melle	264:268	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	5	41	theme	17.826	899:904	arg1	%					905:905	%	905:905	%	905:905	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	7	42	theme	composition	1324:1334	arg1	ratio					1336:1340	the composition ratio	1320:1340	the composition ratio of each type of constituent	1320:1368	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	5	43	theme	free	1007:1010	arg1	acids					1018:1022	free amino acids	1007:1022	2.154% free amino acids	1000:1022	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	2	44	theme	effective	399:407	arg1	way					409:411	an effective way	396:411	an effective way to develop Traditional Chinese medicine and innovative medicines	396:476	Clarifying the active substances is an effective way to develop Traditional Chinese medicine and innovative medicines.
36807552	0	45	theme	chemical	54:61	arg1	composition					63:73	the chemical composition	50:73	the chemical composition of Sijunzi Decoction	50:94	Multiple approaches to characterize and visualize the chemical composition of Sijunzi Decoction comprehensively.
36807552	5	46	theme	powder	850:855	arg1	%					810:810	74.544%	804:810	74.544% of the Sijunzi Decoction freeze-dried powder	804:855	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	46	theme	powder	850:855	arg1	powder					850:855	the Sijunzi Decoction freeze-dried powder	815:855	the Sijunzi Decoction freeze-dried powder	815:855	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	47	theme	amino	1012:1016	arg1	acids					1018:1022	free amino acids	1007:1022	2.154% free amino acids	1000:1022	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	4	48	theme	representative	718:731	arg1	components					733:742	representative components	718:742	representative components	718:742	Molecular network also used for visualizing the ingredients in Sijunzi Decoction, and representative components were also quantified.
36807552	1	49	from	syndrome	330:337	arg1	medicine					350:357	Chinese medicine	342:357	Chinese medicine	342:357	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	5	50	dep	%	1087:1087	arg1	elements					1099:1106	inorganic elements	1089:1106	0.483% inorganic elements	1082:1106	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	51	theme	freeze-dried	837:848	arg1	powder					850:855	the Sijunzi Decoction freeze-dried powder	815:855	the Sijunzi Decoction freeze-dried powder	815:855	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	3	52	theme	amino	504:508	arg1	acids					510:514	amino acids	504:514	amino acids	504:514	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	1	53	theme	spleen	312:317	arg1	syndrome					330:337	spleen deficiency syndrome	312:337	spleen deficiency syndrome in Chinese medicine	312:357	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	7	54	theme	type	1350:1353	arg1	ratio					1336:1340	the composition ratio	1320:1340	the composition ratio of each type of constituent	1320:1368	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	5	55	theme	insoluble	976:984	arg1	precipitates					986:997	insoluble precipitates	976:997	2.427% insoluble precipitates	969:997	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	3	56	from	Carbohydrates	479:491	arg1	decoction					584:592	the decoction	580:592	the decoction	580:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	6	57	theme	quantitative	1131:1142	arg1	analysis					1144:1151	quantitative analysis	1131:1151	quantitative analysis	1131:1151	Molecular network and quantitative analysis used to characterize the chemical composition of Sijunzi Decoction.
36807552	1	58	dep	Glycyrrhizae	219:230	arg1	Et					238:239	Glycyrrhizae Radix Et	219:239	Glycyrrhizae Radix Et	219:239	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	3	59	from	elements	568:575	arg1	decoction					584:592	the decoction	580:592	the decoction	580:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	5	60	dep	%	1055:1055	arg1	acids					1072:1076	total phenolic acids	1057:1076	0.546% total phenolic acids	1050:1076	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	61	theme	detected	770:777	arg1	components					779:788	The detected components	766:788	The detected components	766:788	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	62	theme	total	1032:1036	arg1	flavonoids					1038:1047	total flavonoids	1032:1047	1.177% total flavonoids	1025:1047	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	5	63	theme	%	905:905	arg1	sugars					907:912	17.826% sugars	899:912	17.826% sugars (degree of polymerization 1-2)	899:943	The detected components accounted for 74.544% of the Sijunzi Decoction freeze-dried powder, including 41.751% crude polysaccharides, 17.826% sugars (degree of polymerization 1-2), 8.181% total saponins, 2.427% insoluble precipitates, 2.154% free amino acids, 1.177% total flavonoids, 0.546% total phenolic acids and 0.483% inorganic elements.
36807552	7	64	from	study	1400:1404	arg1	basis					1419:1423	substance basis	1409:1423	substance basis of other Chinese medicine	1409:1449	The present study systematically characterized the constituents of Sijunzi Decoction, and revealed the composition ratio of each type of constituent, and provided a reference for study on substance basis of other Chinese medicine.
36807552	1	65	theme	deficiency	319:328	arg1	syndrome					330:337	spleen deficiency syndrome	312:337	spleen deficiency syndrome in Chinese medicine	312:357	Sijunzi Decoction is composed of Ginseng Radix et Rhizoma, Atractylodes Macrocephalae Rhizoma, Poria, and Glycyrrhizae Radix Et Rhizoma Praeparata Cum Melle, and it is a classic formula for treating spleen deficiency syndrome in Chinese medicine.
36807552	3	66	theme	multiple	611:618	arg1	approaches					620:629	multiple approaches	611:629	multiple approaches	611:629	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
36807552	3	67	from	flavonoids	527:536	arg1	decoction					584:592	the decoction	580:592	the decoction	580:592	Carbohydrates, proteins, amino acids, saponins, flavonoids, phenolic acids and inorganic elements in the decoction were analyzed by multiple approaches.
37099108	0	0	theme	process	85:91	arg1	conditions					93:102	process conditions	85:102	process conditions	85:102	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	3	1	theme	DSS	731:733	arg1	Proteins					633:640	Proteins	633:640	Proteins	633:640	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	1	theme	DSS	731:733	arg1	acids					679:683	volatile fatty acids	664:683	volatile fatty acids (VFAs)	664:690	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	1	theme	DSS	731:733	arg1	products					719:726	the main hydrothermal products	697:726	the main hydrothermal products of DSS	697:733	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	1	theme	DSS	731:733	arg1	polysaccharides					643:657	polysaccharides	643:657	polysaccharides	643:657	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	2	2	theme	maximum	371:377	arg1	yield					387:391	The maximum methane yield	367:391	The maximum methane yield	367:391	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	5	3	theme	nonapatite	1023:1032	arg1	P					1044:1044	nonapatite inorganic P	1023:1044	nonapatite inorganic P	1023:1044	Solid-organic P was converted into liquid-P during the hydrothermal process, and nonapatite inorganic P was converted into organic P during AD.
37099108	7	4	theme	DSS	1395:1397	arg1	digestion					1382:1390	the anaerobic digestion	1368:1390	the anaerobic digestion of DSS	1368:1397	Results showed that the HTP improved the anaerobic digestion of DSS.
37099108	2	5	theme	initial	580:586	arg1	conditions					601:610	the initial hydrothermal conditions	576:610	the initial hydrothermal conditions (A1, 140 °C-1 h-5%)	576:630	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	1	6	theme	hydrothermal	158:169	arg1	characteristics					190:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	6	7	theme	degradation	1254:1264	arg1	community					1266:1274	the anaerobic microbial degradation community	1230:1274	the anaerobic microbial degradation community	1230:1274	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	5	8	theme	inorganic	1034:1042	arg1	P					1044:1044	nonapatite inorganic P	1023:1044	nonapatite inorganic P	1023:1044	Solid-organic P was converted into liquid-P during the hydrothermal process, and nonapatite inorganic P was converted into organic P during AD.
37099108	6	9	theme	A4	1158:1159	arg1	balance					1147:1153	the energy balance	1136:1153	the energy balance of A4	1136:1159	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	6	9	theme	A4	1158:1159	arg1	10.50 kJ/g					1165:1174	10.50 kJ/g	1165:1174	10.50 kJ/g	1165:1174	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	1	10	theme	anaerobic	241:249	arg1	AD					262:263	AD	262:263	AD	262:263	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	1	10	theme	anaerobic	241:249	arg1	digestion					251:259	anaerobic digestion	241:259	anaerobic digestion (AD)	241:264	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	0	11	theme	conditions	93:102	arg1	effects					74:80	effects	74:80	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.	0:152	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	4	12	theme	tryptophan	776:785	arg1	proteins					787:794	tryptophan proteins	776:794	tryptophan proteins	776:794	3D-EEM analysis revealed that tyrosine, tryptophan proteins, and fulvic acids decreased after HTP, but the content of humic acid-like substances increased, and this phenomenon was more noticeable after AD.
37099108	1	13	theme	digestion	251:259	arg1	characteristics					190:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	1	13	theme	digestion	251:259	arg1	fate					214:217	the fate	210:217	the fate of phosphorus (P) and anaerobic digestion (AD)	210:264	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	6	14	theme	anaerobic	1234:1242	arg1	community					1266:1274	the anaerobic microbial degradation community	1230:1274	the anaerobic microbial degradation community	1230:1274	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	1	15	theme	pretreatment	171:182	arg1	characteristics					190:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	0	16	theme	methane	107:113	arg1	production					115:124	methane production	107:124	methane production	107:124	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	1	17	theme	different	332:340	arg1	conditions					355:364	different hydrothermal conditions	332:364	different hydrothermal conditions	332:364	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	4	18	theme	3D-EEM	736:741	arg1	analysis					743:750	3D-EEM analysis	736:750	3D-EEM analysis	736:750	3D-EEM analysis revealed that tyrosine, tryptophan proteins, and fulvic acids decreased after HTP, but the content of humic acid-like substances increased, and this phenomenon was more noticeable after AD.
37099108	3	19	theme	hydrothermal	706:717	arg1	Proteins					633:640	Proteins	633:640	Proteins	633:640	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	19	theme	hydrothermal	706:717	arg1	acids					679:683	volatile fatty acids	664:683	volatile fatty acids (VFAs)	664:690	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	19	theme	hydrothermal	706:717	arg1	products					719:726	the main hydrothermal products	697:726	the main hydrothermal products of DSS	697:733	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	19	theme	hydrothermal	706:717	arg1	polysaccharides					643:657	polysaccharides	643:657	polysaccharides	643:657	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	6	20	theme	organic	1298:1304	arg1	composition					1306:1316	the sludge organic composition	1287:1316	the sludge organic composition	1287:1316	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	1	21	theme	hydrothermal	342:353	arg1	conditions					355:364	different hydrothermal conditions	332:364	different hydrothermal conditions	332:364	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	5	22	theme	organic	1065:1071	arg1	P					1073:1073	organic P	1065:1073	organic P during AD	1065:1083	Solid-organic P was converted into liquid-P during the hydrothermal process, and nonapatite inorganic P was converted into organic P during AD.
37099108	1	23	theme	HTP	185:187	arg1	characteristics					190:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	0	24	theme	Anaerobic	0:8	arg1	digestion					10:18	Anaerobic digestion	0:18	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.	0:152	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	3	25	theme	volatile	664:671	arg1	Proteins					633:640	Proteins	633:640	Proteins	633:640	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	25	theme	volatile	664:671	arg1	VFAs					686:689	VFAs	686:689	VFAs	686:689	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	25	theme	volatile	664:671	arg1	products					719:726	the main hydrothermal products	697:726	the main hydrothermal products of DSS	697:733	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	25	theme	volatile	664:671	arg1	acids					679:683	volatile fatty acids	664:683	volatile fatty acids (VFAs)	664:690	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	25	theme	volatile	664:671	arg1	polysaccharides					643:657	polysaccharides	643:657	polysaccharides	643:657	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	1	26	dep	characteristics	190:204	arg1	performance					266:276	performance	266:276	performance	266:276	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	2	27	theme	hydrothermal	588:599	arg1	conditions					601:610	the initial hydrothermal conditions	576:610	the initial hydrothermal conditions (A1, 140 °C-1 h-5%)	576:630	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	6	28	theme	community	1266:1274	arg1	composition					1215:1225	the composition	1211:1225	the composition of the anaerobic microbial degradation community	1211:1274	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	2	29	theme	241 mL	401:406	arg1	COD					414:416	241 mL CH4/g COD	401:416	241 mL CH4/g COD	401:416	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	2	30	theme	CH4/g	408:412	arg1	COD					414:416	241 mL CH4/g COD	401:416	241 mL CH4/g COD	401:416	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	0	31	theme	dewatered	49:57	arg1	sludge					66:71	hydrothermally pretreated dewatered sewage sludge	23:71	hydrothermally pretreated dewatered sewage sludge	23:71	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	0	32	theme	pretreated	38:47	arg1	sludge					66:71	hydrothermally pretreated dewatered sewage sludge	23:71	hydrothermally pretreated dewatered sewage sludge	23:71	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	4	33	theme	substances	870:879	arg1	content					843:849	the content	839:849	the content of humic acid-like substances	839:879	3D-EEM analysis revealed that tyrosine, tryptophan proteins, and fulvic acids decreased after HTP, but the content of humic acid-like substances increased, and this phenomenon was more noticeable after AD.
37099108	0	34	from	effects	74:80	arg1	fate					134:137	the fate	130:137	the fate of phosphorus	130:151	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	0	34	from	effects	74:80	arg1	production					115:124	methane production	107:124	methane production	107:124	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	2	35	theme	methane	379:385	arg1	yield					387:391	The maximum methane yield	367:391	The maximum methane yield	367:391	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	0	36	theme	sludge	66:71	arg1	digestion					10:18	Anaerobic digestion	0:18	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.	0:152	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	1	37	theme	dewatered	281:289	arg1	DSS					306:308	DSS	306:308	DSS	306:308	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	1	37	theme	dewatered	281:289	arg1	sludge					298:303	dewatered sewage sludge	281:303	dewatered sewage sludge (DSS)	281:309	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	3	38	theme	fatty	673:677	arg1	Proteins					633:640	Proteins	633:640	Proteins	633:640	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	38	theme	fatty	673:677	arg1	VFAs					686:689	VFAs	686:689	VFAs	686:689	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	38	theme	fatty	673:677	arg1	products					719:726	the main hydrothermal products	697:726	the main hydrothermal products of DSS	697:733	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	38	theme	fatty	673:677	arg1	acids					679:683	volatile fatty acids	664:683	volatile fatty acids (VFAs)	664:690	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	38	theme	fatty	673:677	arg1	polysaccharides					643:657	polysaccharides	643:657	polysaccharides	643:657	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	6	39	theme	energy	1116:1121	arg1	balance					1123:1129	positive energy balance	1107:1129	positive energy balance	1107:1129	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	5	40	theme	hydrothermal	997:1008	arg1	process					1010:1016	the hydrothermal process	993:1016	the hydrothermal process	993:1016	Solid-organic P was converted into liquid-P during the hydrothermal process, and nonapatite inorganic P was converted into organic P during AD.
37099108	0	41	theme	sewage	59:64	arg1	sludge					66:71	hydrothermally pretreated dewatered sewage sludge	23:71	hydrothermally pretreated dewatered sewage sludge	23:71	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	1	42	theme	sewage	291:296	arg1	DSS					306:308	DSS	306:308	DSS	306:308	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	1	42	theme	sewage	291:296	arg1	sludge					298:303	dewatered sewage sludge	281:303	dewatered sewage sludge (DSS)	281:309	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	0	43	theme	phosphorus	142:151	arg1	fate					134:137	the fate	130:137	the fate of phosphorus	130:151	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	0	43	theme	phosphorus	142:151	arg1	production					115:124	methane production	107:124	methane production	107:124	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	2	44	theme	hydrothermal	427:438	arg1	conditions					440:449	the hydrothermal conditions	423:449	the hydrothermal conditions	423:449	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	2	44	theme	hydrothermal	427:438	arg1	%					469:469	200 °C-2 h-10%	456:469	200 °C-2 h-10% (A4)	456:474	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	6	45	theme	energy	1140:1145	arg1	balance					1147:1153	the energy balance	1136:1153	the energy balance of A4	1136:1159	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	6	45	theme	energy	1140:1145	arg1	10.50 kJ/g					1165:1174	10.50 kJ/g	1165:1174	10.50 kJ/g	1165:1174	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	2	46	dep	conditions	601:610	arg1	%					629:629	140 °C-1 h-5%	617:629	140 °C-1 h-5%	617:629	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	2	46	dep	conditions	601:610	arg1	A1					613:614	A1	613:614	A1	613:614	The maximum methane yield reached 241 mL CH4/g COD when the hydrothermal conditions were 200 °C-2 h-10% (A4), and the yield was 78.28% higher than that without pretreatment (A0) and 29.62% higher than that of the initial hydrothermal conditions (A1, 140 °C-1 h-5%).
37099108	1	47	theme	sludge	298:303	arg1	characteristics					190:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	1	47	theme	sludge	298:303	arg1	fate					214:217	the fate	210:217	the fate of phosphorus (P) and anaerobic digestion (AD)	210:264	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	6	48	theme	Microbial	1180:1188	arg1	analysis					1190:1197	Microbial analysis	1180:1197	Microbial analysis	1180:1197	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	7	49	theme	anaerobic	1372:1380	arg1	digestion					1382:1390	the anaerobic digestion	1368:1390	the anaerobic digestion of DSS	1368:1397	Results showed that the HTP improved the anaerobic digestion of DSS.
37099108	3	50	theme	main	701:704	arg1	Proteins					633:640	Proteins	633:640	Proteins	633:640	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	50	theme	main	701:704	arg1	acids					679:683	volatile fatty acids	664:683	volatile fatty acids (VFAs)	664:690	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	50	theme	main	701:704	arg1	products					719:726	the main hydrothermal products	697:726	the main hydrothermal products of DSS	697:733	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	3	50	theme	main	701:704	arg1	polysaccharides					643:657	polysaccharides	643:657	polysaccharides	643:657	Proteins, polysaccharides, and volatile fatty acids (VFAs) were the main hydrothermal products of DSS.
37099108	4	51	theme	fulvic	801:806	arg1	acids					808:812	fulvic acids	801:812	fulvic acids	801:812	3D-EEM analysis revealed that tyrosine, tryptophan proteins, and fulvic acids decreased after HTP, but the content of humic acid-like substances increased, and this phenomenon was more noticeable after AD.
37099108	5	52	theme	Solid-organic	942:954	arg1	P					956:956	Solid-organic P	942:956	Solid-organic P	942:956	Solid-organic P was converted into liquid-P during the hydrothermal process, and nonapatite inorganic P was converted into organic P during AD.
37099108	1	53	theme	phosphorus	222:231	arg1	characteristics					190:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics	154:204	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	1	53	theme	phosphorus	222:231	arg1	fate					214:217	the fate	210:217	the fate of phosphorus (P) and anaerobic digestion (AD)	210:264	The hydrothermal pretreatment (HTP) characteristics and the fate of phosphorus (P) and anaerobic digestion (AD) performance of dewatered sewage sludge (DSS) were investigated at different hydrothermal conditions.
37099108	4	54	theme	acid-like	860:868	arg1	substances					870:879	humic acid-like substances	854:879	humic acid-like substances	854:879	3D-EEM analysis revealed that tyrosine, tryptophan proteins, and fulvic acids decreased after HTP, but the content of humic acid-like substances increased, and this phenomenon was more noticeable after AD.
37099108	0	55	dep	digestion	10:18	arg1	effects					74:80	effects	74:80	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.	0:152	Anaerobic digestion of hydrothermally pretreated dewatered sewage sludge: effects of process conditions on methane production and the fate of phosphorus.
37099108	6	56	theme	sludge	1291:1296	arg1	composition					1306:1316	the sludge organic composition	1287:1316	the sludge organic composition	1287:1316	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	4	57	theme	humic	854:858	arg1	substances					870:879	humic acid-like substances	854:879	humic acid-like substances	854:879	3D-EEM analysis revealed that tyrosine, tryptophan proteins, and fulvic acids decreased after HTP, but the content of humic acid-like substances increased, and this phenomenon was more noticeable after AD.
37099108	6	58	theme	positive	1107:1114	arg1	balance					1123:1129	positive energy balance	1107:1129	positive energy balance	1107:1129	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
37099108	6	59	theme	microbial	1244:1252	arg1	community					1266:1274	the anaerobic microbial degradation community	1230:1274	the anaerobic microbial degradation community	1230:1274	All samples achieved positive energy balance, and the energy balance of A4 was 10.50 kJ/g VS. Microbial analysis showed that the composition of the anaerobic microbial degradation community changed as the sludge organic composition was altered.
36245541	8	0	theme	weight	926:931	arg1	changes					954:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	6	1	theme	repeat	773:778	arg1	study					799:803	a 14-day repeat dose oral toxicity study	764:803	a 14-day repeat dose oral toxicity study	764:803	The acute toxicity study was carried out by a 14-day repeat dose oral toxicity study.
36245541	0	2	theme	Cyclocarya	66:75	arg1	extracts					54:61	aqueous extracts	46:61	aqueous extracts of Cyclocarya paliurus	46:84	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	8	3	theme	body	921:924	arg1	weight					926:931	body weight	921:931	body weight	921:931	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	6	4	theme	acute	724:728	arg1	study					739:743	The acute toxicity study	720:743	The acute toxicity study	720:743	The acute toxicity study was carried out by a 14-day repeat dose oral toxicity study.
36245541	1	5	used	used	176:179	arg2	ingredients					210:220	ingredients	210:220	ingredients in functional foods	210:240	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	1	5	used	used	176:179	arg2	Cyclocarya					94:103	Cyclocarya	94:103	Cyclocarya	94:103	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	3	6	dep	dose	367:370	arg1	leaves					440:445	leaves	440:445	leaves	440:445	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	6	7	theme	toxicity	790:797	arg1	study					799:803	a 14-day repeat dose oral toxicity study	764:803	a 14-day repeat dose oral toxicity study	764:803	The acute toxicity study was carried out by a 14-day repeat dose oral toxicity study.
36245541	10	8	used	used	1268:1271	arg2	AECPL					1242:1246	AECPL	1242:1246	AECPL	1242:1246	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	3	9	theme	present	331:337	arg1	work					339:342	the present work	327:342	the present work	327:342	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	6	10	theme	oral	785:788	arg1	study					799:803	a 14-day repeat dose oral toxicity study	764:803	a 14-day repeat dose oral toxicity study	764:803	The acute toxicity study was carried out by a 14-day repeat dose oral toxicity study.
36245541	8	11	theme	consumption	942:952	arg1	changes					954:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	3	12	theme	paliurus	431:438	arg1	extracts					416:423	the aqueous extracts	404:423	the aqueous extracts of C. paliurus	404:438	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	5	13	theme	male	698:701	arg1	rats					714:717	both male and female rats	693:717	both male and female rats	693:717	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	5	14	theme	>	672:672	arg1	BW					687:688	> 10,000 mg/kg BW	672:688	> 10,000 mg/kg BW in both male and female rats	672:717	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	5	14	theme	>	672:672	arg1	MTD					638:640	The MTD	634:640	The MTD of AECPL	634:649	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	6	15	theme	dose	780:783	arg1	study					799:803	a 14-day repeat dose oral toxicity study	764:803	a 14-day repeat dose oral toxicity study	764:803	The acute toxicity study was carried out by a 14-day repeat dose oral toxicity study.
36245541	8	16	theme	food	937:940	arg1	consumption					942:952	food consumption	937:952	food consumption	937:952	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	8	17	theme	clinical	905:912	arg1	signs					914:918	abnormal clinical signs	896:918	abnormal clinical signs	896:918	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	4	18	dep	paliurus	617:624	arg1	leaves					626:631	leaves	626:631	leaves	626:631	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	3	19	theme	aqueous	408:414	arg1	extracts					416:423	the aqueous extracts	404:423	the aqueous extracts of C. paliurus	404:438	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	0	20	theme	chemical	4:11	arg1	composition					13:23	chemical composition	4:23	chemical composition	4:23	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	4	21	from	polysaccharides	543:557	arg1	rich					508:511	rich	508:511	rich	508:511	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	8	22	theme	abnormal	896:903	arg1	signs					914:918	abnormal clinical signs	896:918	abnormal clinical signs	896:918	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	10	23	from	BW/day	1223:1228	arg1	study					1153:1157	this study	1148:1157	this study	1148:1157	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	9	24	theme	organ	1095:1099	arg1	hematology					1043:1052	hematology	1043:1052	hematology	1043:1052	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	24	theme	organ	1095:1099	arg1	weights					1101:1107	organ weights	1095:1107	organ weights	1095:1107	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	0	25	dep	Cyclocarya	66:75	arg1	paliurus					77:84	Cyclocarya paliurus	66:84	Cyclocarya paliurus	66:84	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	9	26	from	changes	1028:1034	arg1	finding					1116:1122	gross finding	1110:1122	gross finding	1110:1122	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	26	from	changes	1028:1034	arg1	hematology					1043:1052	hematology	1043:1052	hematology	1043:1052	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	26	from	changes	1028:1034	arg1	parameters					1083:1092	urinalysis parameters	1072:1092	urinalysis parameters	1072:1092	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	26	from	changes	1028:1034	arg1	weights					1101:1107	organ weights	1095:1107	organ weights	1095:1107	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	26	from	changes	1028:1034	arg1	histopathology					1129:1142	histopathology	1129:1142	histopathology	1129:1142	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	26	from	changes	1028:1034	arg1	chemistry					1061:1069	serum chemistry	1055:1069	serum chemistry	1055:1069	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	3	27	theme	maximum	349:355	arg1	dose					367:370	the maximum tolerated dose	345:370	the maximum tolerated dose (MTD)	345:376	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	27	theme	maximum	349:355	arg1	AECPL					448:452	AECPL	448:452	AECPL	448:452	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	27	theme	maximum	349:355	arg1	MTD					373:375	MTD	373:375	MTD	373:375	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	8	28	theme	mortality	885:893	arg1	changes					954:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	8	29	theme	treatment-related	867:883	arg1	changes					954:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	1	30	theme	dietary	184:190	arg1	supplements					192:202	dietary supplements	184:202	dietary supplements	184:202	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	3	31	theme	tolerated	357:365	arg1	dose					367:370	the maximum tolerated dose	345:370	the maximum tolerated dose (MTD)	345:376	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	31	theme	tolerated	357:365	arg1	AECPL					448:452	AECPL	448:452	AECPL	448:452	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	31	theme	tolerated	357:365	arg1	MTD					373:375	MTD	373:375	MTD	373:375	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	10	32	theme	5,000	1211:1215	arg1	mg/kg					1217:1221	mg/kg	1217:1221	mg/kg	1217:1221	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	6	33	theme	14-day	766:771	arg1	study					799:803	a 14-day repeat dose oral toxicity study	764:803	a 14-day repeat dose oral toxicity study	764:803	The acute toxicity study was carried out by a 14-day repeat dose oral toxicity study.
36245541	1	34	theme	various	136:142	arg1	bioactivities					144:156	various bioactivities	136:156	various bioactivities	136:156	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	5	35	theme	AECPL	645:649	arg1	BW					687:688	> 10,000 mg/kg BW	672:688	> 10,000 mg/kg BW in both male and female rats	672:717	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	5	35	theme	AECPL	645:649	arg1	MTD					638:640	The MTD	634:640	The MTD of AECPL	634:649	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	9	36	theme	gross	1110:1114	arg1	finding					1116:1122	gross finding	1110:1122	gross finding	1110:1122	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	36	theme	gross	1110:1114	arg1	hematology					1043:1052	hematology	1043:1052	hematology	1043:1052	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	10	37	theme	non-observed-adverse-effect	1164:1190	arg1	level					1192:1196	the non-observed-adverse-effect level	1160:1196	the non-observed-adverse-effect level of AECPL	1160:1205	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	10	37	theme	non-observed-adverse-effect	1164:1190	arg1	BW/day					1223:1228	5,000 mg/kg BW/day	1211:1228	5,000 mg/kg BW/day	1211:1228	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	3	38	theme	extracts	416:423	arg1	dose					367:370	the maximum tolerated dose	345:370	the maximum tolerated dose (MTD)	345:376	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	38	theme	extracts	416:423	arg1	AECPL					448:452	AECPL	448:452	AECPL	448:452	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	38	theme	extracts	416:423	arg1	toxicity					392:399	potential toxicity	382:399	potential toxicity	382:399	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	38	theme	extracts	416:423	arg1	MTD					373:375	MTD	373:375	MTD	373:375	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	3	39	theme	potential	382:390	arg1	toxicity					392:399	potential toxicity	382:399	potential toxicity	382:399	In the present work, the maximum tolerated dose (MTD) and potential toxicity of the aqueous extracts of C. paliurus leaves (AECPL) were evaluated.
36245541	0	40	theme	toxic	29:33	arg1	effects					35:41	toxic effects	29:41	toxic effects	29:41	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	9	41	theme	serum	1055:1059	arg1	hematology					1043:1052	hematology	1043:1052	hematology	1043:1052	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	41	theme	serum	1055:1059	arg1	chemistry					1061:1069	serum chemistry	1055:1069	serum chemistry	1055:1069	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	4	42	from	rich	508:511	arg1	flavonoids					527:536	flavonoids	527:536	flavonoids	527:536	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	4	42	from	rich	508:511	arg1	polysaccharides					543:557	polysaccharides	543:557	polysaccharides	543:557	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	4	42	from	rich	508:511	arg1	phenolics					516:524	phenolics	516:524	phenolics	516:524	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	0	43	theme	aqueous	46:52	arg1	extracts					54:61	aqueous extracts	46:61	aqueous extracts of Cyclocarya paliurus	46:84	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	5	44	theme	mg/kg	681:685	arg1	BW					687:688	> 10,000 mg/kg BW	672:688	> 10,000 mg/kg BW in both male and female rats	672:717	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	5	44	theme	mg/kg	681:685	arg1	MTD					638:640	The MTD	634:640	The MTD of AECPL	634:649	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	10	45	theme	mg/kg	1217:1221	arg1	level					1192:1196	the non-observed-adverse-effect level	1160:1196	the non-observed-adverse-effect level of AECPL	1160:1205	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	10	45	theme	mg/kg	1217:1221	arg1	BW/day					1223:1228	5,000 mg/kg BW/day	1211:1228	5,000 mg/kg BW/day	1211:1228	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	2	46	dep	toxicity	295:302	arg1	concerns					314:321	concerns	314:321	concerns	314:321	However, limited information is available about the toxicity or safety concerns.
36245541	2	46	dep	toxicity	295:302	arg1	the					291:293	the	291:293	the	291:293	However, limited information is available about the toxicity or safety concerns.
36245541	5	47	theme	female	707:712	arg1	rats					714:717	both male and female rats	693:717	both male and female rats	693:717	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	4	48	from	phenolics	516:524	arg1	rich					508:511	rich	508:511	rich	508:511	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	5	49	from	BW	687:688	arg1	rats					714:717	both male and female rats	693:717	both male and female rats	693:717	The MTD of AECPL was considered to be > 10,000 mg/kg BW in both male and female rats.
36245541	9	50	theme	adverse	1020:1026	arg1	changes					1028:1034	no adverse changes	1017:1034	no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology	1017:1142	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	8	51	theme	signs	914:918	arg1	changes					954:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes	864:960	No treatment-related mortality, abnormal clinical signs, body weight, or food consumption changes were reported during the study.
36245541	1	52	from	ingredients	210:220	arg1	foods					236:240	functional foods	225:240	functional foods	225:240	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	10	53	theme	food	1280:1283	arg1	industry					1285:1292	the food industry	1276:1292	the food industry	1276:1292	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	2	54	theme	limited	252:258	arg1	information					260:270	limited information	252:270	limited information	252:270	However, limited information is available about the toxicity or safety concerns.
36245541	4	55	from	flavonoids	527:536	arg1	rich					508:511	rich	508:511	rich	508:511	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	0	56	dep	composition	13:23	arg1	The					0:2	The	0:2	The	0:2	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	9	57	theme	urinalysis	1072:1081	arg1	hematology					1043:1052	hematology	1043:1052	hematology	1043:1052	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	9	57	theme	urinalysis	1072:1081	arg1	parameters					1083:1092	urinalysis parameters	1072:1092	urinalysis parameters	1072:1092	Moreover, AECPL showed no adverse changes in the hematology, serum chemistry, urinalysis parameters, organ weights, gross finding, and histopathology.
36245541	6	58	theme	toxicity	730:737	arg1	study					739:743	The acute toxicity study	720:743	The acute toxicity study	720:743	The acute toxicity study was carried out by a 14-day repeat dose oral toxicity study.
36245541	10	59	theme	AECPL	1201:1205	arg1	level					1192:1196	the non-observed-adverse-effect level	1160:1196	the non-observed-adverse-effect level of AECPL	1160:1205	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	10	59	theme	AECPL	1201:1205	arg1	BW/day					1223:1228	5,000 mg/kg BW/day	1211:1228	5,000 mg/kg BW/day	1211:1228	In this study, the non-observed-adverse-effect level of AECPL was 5,000 mg/kg BW/day, indicating AECPL was safe and can be used in the food industry.
36245541	1	60	dep	Cyclocarya	94:103	arg1	leaves					114:119	leaves	114:119	leaves	114:119	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	1	60	dep	Cyclocarya	94:103	arg1	paliurus					105:112	Cyclocarya paliurus	94:112	Cyclocarya paliurus leaves	94:119	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	4	61	theme	health	595:600	arg1	benefits					602:609	the health benefits	591:609	the health benefits of C. paliurus leaves	591:631	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36245541	1	62	theme	functional	225:234	arg1	foods					236:240	functional foods	225:240	functional foods	225:240	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	0	63	theme	extracts	54:61	arg1	composition					13:23	chemical composition	4:23	chemical composition	4:23	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	0	63	theme	extracts	54:61	arg1	effects					35:41	toxic effects	29:41	toxic effects	29:41	The chemical composition and toxic effects of aqueous extracts of Cyclocarya paliurus leaves.
36245541	1	64	contain	possess	128:134	arg2	bioactivities					144:156	various bioactivities	136:156	various bioactivities	136:156	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	1	64	contain	possess	128:134	arg1	Cyclocarya					94:103	Cyclocarya	94:103	Cyclocarya	94:103	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	1	64	contain	possess	128:134	arg1	ingredients					210:220	ingredients	210:220	ingredients in functional foods	210:240	Cyclocarya paliurus leaves, which possess various bioactivities, have been widely used in dietary supplements or as ingredients in functional foods.
36245541	4	65	theme	paliurus	617:624	arg1	benefits					602:609	the health benefits	591:609	the health benefits of C. paliurus leaves	591:631	Our results indicated that AECPL was rich in phenolics, flavonoids, and polysaccharides, which might be responsible for the health benefits of C. paliurus leaves.
36832826	8	0	theme	UC	1586:1587	arg1	management					1589:1598	UC management	1586:1598	UC management	1586:1598	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	3	1	theme	×	631:631	arg1	kDa					637:639	7.70 × 103 and 1.76 × 102 kDa	611:639	kDa	637:639	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	1	2	theme	plant	162:166	arg1	humifusa					148:155	Euphorbia humifusa	138:155	Euphorbia humifusa	138:155	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	1	2	theme	plant	162:166	arg1	species					168:174	a plant species	160:174	a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases	160:267	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	0	3	theme	Protective	84:93	arg1	Effect					95:100	Their Protective Effect	78:100	Their Protective Effect against Ulcerative Colitis in Mice	78:135	In Vitro Fecal Fermentation of Euphorbia humifusa-Derived Polysaccharides and Their Protective Effect against Ulcerative Colitis in Mice.
36832826	1	4	with	species	168:174	arg1	characteristics					200:214	medicinal and food characteristics	181:214	medicinal and food characteristics used to treat diarrhea and other intestinal diseases	181:267	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	2	5	theme	human	365:369	arg1	microbiota					379:388	human colonic microbiota	365:388	human colonic microbiota	365:388	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	4	6	dep	identified	666:675	arg1	verified					712:719	verified	712:719	verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers	712:838	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	0	7	theme	Ulcerative	110:119	arg1	Colitis					121:127	Ulcerative Colitis	110:127	Ulcerative Colitis in Mice	110:135	In Vitro Fecal Fermentation of Euphorbia humifusa-Derived Polysaccharides and Their Protective Effect against Ulcerative Colitis in Mice.
36832826	5	8	dep	in	848:849	arg1	vitro					851:855	vitro	851:855	vitro	851:855	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	4	9	theme	cell	824:827	arg1	monolayers					829:838	Caco-2 cell monolayers	817:838	Caco-2 cell monolayers	817:838	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	7	10	theme	-induced	1300:1307	arg1	model					1318:1322	a dextran sulfate sodium (DSS)-induced UC mouse model	1270:1322	a dextran sulfate sodium (DSS)-induced UC mouse model	1270:1322	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	5	11	theme	valeric	918:924	arg1	acids					926:930	acetic, propionic, and valeric acids	895:930	acetic, propionic, and valeric acids	895:930	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	2	12	theme	prebiotic	298:306	arg1	effects					308:314	the prebiotic effects	294:314	the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota	294:388	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	13	theme	colonic	371:377	arg1	microbiota					379:388	human colonic microbiota	365:388	human colonic microbiota	365:388	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	8	14	used	utilized	1512:1519	arg2	strategy					1573:1580	a promising nutritional strategy	1549:1580	a promising nutritional strategy for UC management	1549:1598	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	8	14	used	utilized	1512:1519	arg2	prebiotic					1536:1544	a potential prebiotic	1524:1544	a potential prebiotic	1524:1544	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	8	14	used	utilized	1512:1519	arg2	EHPs					1498:1501	EHPs	1498:1501	EHPs	1498:1501	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	4	15	theme	coefficient	750:760	arg1	cm/s					788:791	Papp < 1.0 × 10-6 cm/s	770:791	Papp < 1.0 × 10-6 cm/s	770:791	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	4	15	theme	coefficient	750:760	arg1	values					762:767	the apparent permeability coefficient values	724:767	the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s)	724:792	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	6	16	theme	microbiota	1077:1086	arg1	composition					1088:1098	the intestinal microbiota composition	1062:1098	the intestinal microbiota composition	1062:1098	Moreover, EHPs could alter the intestinal microbiota composition by increasing the relative abundance of Bifidobacterium and Holdemanella and reducing that of Escherichia-Shigella, Tyzzerella, and Parasutterella at the genus level.
36832826	7	17	theme	colon	1399:1403	arg1	damage					1412:1417	the colon tissue damage	1395:1417	the colon tissue damage	1395:1417	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	7	18	theme	UC	1309:1310	arg1	model					1318:1322	a dextran sulfate sodium (DSS)-induced UC mouse model	1270:1322	a dextran sulfate sodium (DSS)-induced UC mouse model	1270:1322	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	3	19	theme	Structural	447:456	arg1	characterization					458:473	Structural characterization	447:473	Structural characterization	447:473	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	5	20	theme	acetic	895:900	arg1	acids					926:930	acetic, propionic, and valeric acids	895:930	acetic, propionic, and valeric acids	895:930	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	7	21	theme	tissue	1405:1410	arg1	damage					1412:1417	the colon tissue damage	1395:1417	the colon tissue damage	1395:1417	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	6	22	theme	intestinal	1066:1075	arg1	composition					1088:1098	the intestinal microbiota composition	1062:1098	the intestinal microbiota composition	1062:1098	Moreover, EHPs could alter the intestinal microbiota composition by increasing the relative abundance of Bifidobacterium and Holdemanella and reducing that of Escherichia-Shigella, Tyzzerella, and Parasutterella at the genus level.
36832826	1	23	theme	medicinal	181:189	arg1	characteristics					200:214	medicinal and food characteristics	181:214	medicinal and food characteristics used to treat diarrhea and other intestinal diseases	181:267	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	6	24	theme	Holdemanella	1160:1171	arg1	abundance					1127:1135	the relative abundance	1114:1135	the relative abundance of Bifidobacterium and Holdemanella	1114:1171	Moreover, EHPs could alter the intestinal microbiota composition by increasing the relative abundance of Bifidobacterium and Holdemanella and reducing that of Escherichia-Shigella, Tyzzerella, and Parasutterella at the genus level.
36832826	2	25	theme	polysaccharides	339:353	arg1	effects					411:417	their regulatory effects	394:417	their regulatory effects on ulcerative colitis (UC)	394:444	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	25	theme	polysaccharides	339:353	arg1	effects					308:314	the prebiotic effects	294:314	the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota	294:388	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	26	theme	regulatory	400:409	arg1	effects					411:417	their regulatory effects	394:417	their regulatory effects on ulcerative colitis (UC)	394:444	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	0	27	theme	In	0:1	arg1	Fermentation					15:26	In Vitro Fecal Fermentation	0:26	In Vitro Fecal Fermentation of Euphorbia	0:39	In Vitro Fecal Fermentation of Euphorbia humifusa-Derived Polysaccharides and Their Protective Effect against Ulcerative Colitis in Mice.
36832826	6	28	theme	genus	1254:1258	arg1	level					1260:1264	the genus level	1250:1264	the genus level	1250:1264	Moreover, EHPs could alter the intestinal microbiota composition by increasing the relative abundance of Bifidobacterium and Holdemanella and reducing that of Escherichia-Shigella, Tyzzerella, and Parasutterella at the genus level.
36832826	2	29	theme	humifusa-derived	322:337	arg1	EHPs					356:359	EHPs	356:359	EHPs	356:359	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	29	theme	humifusa-derived	322:337	arg1	polysaccharides					339:353	humifusa-derived polysaccharides	322:353	E. humifusa-derived polysaccharides (EHPs)	319:360	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	1	30	theme	food	195:198	arg1	characteristics					200:214	medicinal and food characteristics	181:214	medicinal and food characteristics used to treat diarrhea and other intestinal diseases	181:267	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	3	31	contain	having	583:588	arg1	heteropolysaccharides					561:581	heteropolysaccharides	561:581	heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa	561:639	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	3	31	contain	having	583:588	arg2	weights					600:606	molecular weights	590:606	molecular weights of 7.70 × 103 and 1.76 × 102 kDa	590:639	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	4	32	theme	Caco-2	817:822	arg1	monolayers					829:838	Caco-2 cell monolayers	817:838	Caco-2 cell monolayers	817:838	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	4	33	theme	Papp	770:773	arg1	cm/s					788:791	Papp < 1.0 × 10-6 cm/s	770:791	Papp < 1.0 × 10-6 cm/s	770:791	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	4	33	theme	Papp	770:773	arg1	values					762:767	the apparent permeability coefficient values	724:767	the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s)	724:792	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	0	34	theme	Fecal	9:13	arg1	Fermentation					15:26	In Vitro Fecal Fermentation	0:26	In Vitro Fecal Fermentation of Euphorbia	0:39	In Vitro Fecal Fermentation of Euphorbia humifusa-Derived Polysaccharides and Their Protective Effect against Ulcerative Colitis in Mice.
36832826	5	35	theme	acids	926:930	arg1	contents					883:890	the contents	879:890	the contents of acetic, propionic, and valeric acids	879:930	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	3	36	theme	molecular	590:598	arg1	weights					600:606	molecular weights	590:606	molecular weights of 7.70 × 103 and 1.76 × 102 kDa	590:639	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	5	37	theme	EHP-supplemented	959:974	arg1	samples					976:982	EHP-supplemented samples	959:982	EHP-supplemented samples	959:982	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	0	38	theme	Euphorbia	31:39	arg1	Fermentation					15:26	In Vitro Fecal Fermentation	0:26	In Vitro Fecal Fermentation of Euphorbia	0:39	In Vitro Fecal Fermentation of Euphorbia humifusa-Derived Polysaccharides and Their Protective Effect against Ulcerative Colitis in Mice.
36832826	5	39	theme	propionic	903:911	arg1	acids					926:930	acetic, propionic, and valeric acids	895:930	acetic, propionic, and valeric acids	895:930	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	6	40	theme	relative	1118:1125	arg1	abundance					1127:1135	the relative abundance	1114:1135	the relative abundance of Bifidobacterium and Holdemanella	1114:1171	Moreover, EHPs could alter the intestinal microbiota composition by increasing the relative abundance of Bifidobacterium and Holdemanella and reducing that of Escherichia-Shigella, Tyzzerella, and Parasutterella at the genus level.
36832826	7	41	theme	mouse	1312:1316	arg1	model					1318:1322	a dextran sulfate sodium (DSS)-induced UC mouse model	1270:1322	a dextran sulfate sodium (DSS)-induced UC mouse model	1270:1322	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	8	42	theme	nutritional	1561:1571	arg1	EHPs					1498:1501	EHPs	1498:1501	EHPs	1498:1501	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	8	42	theme	nutritional	1561:1571	arg1	strategy					1573:1580	a promising nutritional strategy	1549:1580	a promising nutritional strategy for UC management	1549:1598	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	7	43	theme	colon	1371:1375	arg1	length					1377:1382	the colon length	1367:1382	the colon length	1367:1382	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	2	44	theme	ulcerative	422:431	arg1	colitis					433:439	ulcerative colitis	422:439	ulcerative colitis (UC)	422:444	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	44	theme	ulcerative	422:431	arg1	UC					442:443	UC	442:443	UC	442:443	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	8	45	theme	promising	1551:1559	arg1	EHPs					1498:1501	EHPs	1498:1501	EHPs	1498:1501	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	8	45	theme	promising	1551:1559	arg1	strategy					1573:1580	a promising nutritional strategy	1549:1580	a promising nutritional strategy for UC management	1549:1598	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	8	46	theme	potential	1526:1534	arg1	EHPs					1498:1501	EHPs	1498:1501	EHPs	1498:1501	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	8	46	theme	potential	1526:1534	arg1	prebiotic					1536:1544	a potential prebiotic	1524:1544	a potential prebiotic	1524:1544	Overall, these results suggest that EHPs could be utilized as a potential prebiotic or a promising nutritional strategy for UC management.
36832826	3	47	theme	7.70	611:614	arg1	×					616:616	7.70 × 103 and 1.76 × 102 kDa	611:639	×	616:616	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	5	48	theme	control	1019:1025	arg1	sample					1027:1032	the control sample	1015:1032	the control sample	1015:1032	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	3	49	theme	×	616:616	arg1	weights					600:606	molecular weights	590:606	molecular weights of 7.70 × 103 and 1.76 × 102 kDa	590:639	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	4	50	theme	permeability	737:748	arg1	cm/s					788:791	Papp < 1.0 × 10-6 cm/s	770:791	Papp < 1.0 × 10-6 cm/s	770:791	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	4	50	theme	permeability	737:748	arg1	values					762:767	the apparent permeability coefficient values	724:767	the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s)	724:792	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	2	51	link	humifusa-derived	322:337	arg1	EHPs					356:359	EHPs	356:359	EHPs	356:359	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	51	link	humifusa-derived	322:337	arg1	polysaccharides					339:353	humifusa-derived polysaccharides	322:353	E. humifusa-derived polysaccharides (EHPs)	319:360	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	0	52	from	Colitis	121:127	arg1	Mice					132:135	Mice	132:135	Mice	132:135	In Vitro Fecal Fermentation of Euphorbia humifusa-Derived Polysaccharides and Their Protective Effect against Ulcerative Colitis in Mice.
36832826	7	53	theme	UC	1341:1342	arg1	symptoms					1344:1351	UC symptoms	1341:1351	UC symptoms	1341:1351	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	2	54	from	effects	411:417	arg1	colitis					433:439	ulcerative colitis	422:439	ulcerative colitis (UC)	422:444	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	54	from	effects	411:417	arg1	microbiota					379:388	human colonic microbiota	365:388	human colonic microbiota	365:388	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	54	from	effects	411:417	arg1	UC					442:443	UC	442:443	UC	442:443	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	1	55	theme	Euphorbia	138:146	arg1	humifusa					148:155	Euphorbia humifusa	138:155	Euphorbia humifusa	138:155	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	1	55	theme	Euphorbia	138:146	arg1	species					168:174	a plant species	160:174	a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases	160:267	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	6	56	theme	Bifidobacterium	1140:1154	arg1	abundance					1127:1135	the relative abundance	1114:1135	the relative abundance of Bifidobacterium and Holdemanella	1114:1171	Moreover, EHPs could alter the intestinal microbiota composition by increasing the relative abundance of Bifidobacterium and Holdemanella and reducing that of Escherichia-Shigella, Tyzzerella, and Parasutterella at the genus level.
36832826	3	57	theme	kDa	637:639	arg1	weights					600:606	molecular weights	590:606	molecular weights of 7.70 × 103 and 1.76 × 102 kDa	590:639	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	4	58	theme	apparent	728:735	arg1	cm/s					788:791	Papp < 1.0 × 10-6 cm/s	770:791	Papp < 1.0 × 10-6 cm/s	770:791	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	4	58	theme	apparent	728:735	arg1	values					762:767	the apparent permeability coefficient values	724:767	the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s)	724:792	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	2	59	from	effects	308:314	arg1	colitis					433:439	ulcerative colitis	422:439	ulcerative colitis (UC)	422:444	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	59	from	effects	308:314	arg1	microbiota					379:388	human colonic microbiota	365:388	human colonic microbiota	365:388	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	59	from	effects	308:314	arg1	UC					442:443	UC	442:443	UC	442:443	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	2	60	dep	polysaccharides	339:353	arg1	E.					319:320	E. humifusa-derived polysaccharides (EHPs)	319:360	E. humifusa-derived polysaccharides (EHPs)	319:360	This study investigated the prebiotic effects of E. humifusa-derived polysaccharides (EHPs) on human colonic microbiota and their regulatory effects on ulcerative colitis (UC).
36832826	4	61	theme	absorbed	687:694	arg1	macromolecules					696:709	poorly absorbed macromolecules	680:709	poorly absorbed macromolecules	680:709	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	5	62	theme	in	848:849	arg1	studies					870:876	in vitro fermentation studies	848:876	in vitro fermentation studies	848:876	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	4	63	theme	cellular	798:805	arg1	uptake					807:812	cellular uptake	798:812	cellular uptake	798:812	EHPs were identified as poorly absorbed macromolecules, verified by the apparent permeability coefficient values (Papp < 1.0 × 10-6 cm/s) and cellular uptake by Caco-2 cell monolayers.
36832826	5	64	theme	fermentation	857:868	arg1	studies					870:876	in vitro fermentation studies	848:876	in vitro fermentation studies	848:876	During in vitro fermentation studies, the contents of acetic, propionic, and valeric acids increased significantly in EHP-supplemented samples after 24 h compared to that in the control sample.
36832826	1	65	theme	other	243:247	arg1	diseases					260:267	other intestinal diseases	243:267	other intestinal diseases	243:267	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
36832826	7	66	theme	pro-inflammatory	1434:1449	arg1	cytokines					1451:1459	pro-inflammatory cytokines	1434:1459	pro-inflammatory cytokines	1434:1459	In a dextran sulfate sodium (DSS)-induced UC mouse model, EHPs alleviated UC symptoms by increasing the colon length, reversing the colon tissue damage and inhibiting pro-inflammatory cytokines.
36832826	0	67	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Fecal Fermentation of Euphorbia humifusa-Derived Polysaccharides and Their Protective Effect against Ulcerative Colitis in Mice.
36832826	3	68	theme	glucuronic	536:545	arg1	acid					547:550	glucuronic acid	536:550	glucuronic acid	536:550	Structural characterization showed that EHPs mainly consisted of galactose, glucose, and glucuronic acid and were heteropolysaccharides having molecular weights of 7.70 × 103 and 1.76 × 102 kDa, respectively.
36832826	1	69	theme	intestinal	249:258	arg1	diseases					260:267	other intestinal diseases	243:267	other intestinal diseases	243:267	Euphorbia humifusa is a plant species with medicinal and food characteristics used to treat diarrhea and other intestinal diseases.
33843779	4	0	theme	agent	450:454	arg1	disposal					425:432	a swift disposal	417:432	a swift disposal of the invading agent by innate immune cells with the least tissue damage	417:506	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	4	0	theme	agent	450:454	arg1	antibody					404:411	a potent neutralizing antibody	382:411	a potent neutralizing antibody	382:411	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	9	1	theme	inflammatory	1574:1585	arg1	syndrome					1596:1603	systemic inflammatory response syndrome	1565:1603	systemic inflammatory response syndrome	1565:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	8	2	theme	different	1269:1277	arg1	mechanisms					1279:1288	the different mechanisms	1265:1288	the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage	1265:1410	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	1	3	from	COVID-19	49:56	arg1	Lessons					36:42	Lessons	36:42	Lessons From COVID-19 for the Rheumatologist	36:79	Lessons From COVID-19 for the Rheumatologist.
33843779	3	4	theme	fight	270:274	arg1	infection					276:284	fight infection	270:284	fight infection	270:284	One side recognizes the microbial antigen, and the other gives a call to arms to fight infection by recruiting immune cells and triggering inflammation.
33843779	4	5	theme	immune	466:471	arg1	cells					473:477	innate immune cells	459:477	innate immune cells with the least tissue damage	459:506	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	6	6	theme	posttranslational	772:788	arg1	modifications					790:802	posttranslational modifications	772:802	posttranslational modifications altering the N-glycan composition in Fc fractions	772:852	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	4	7	theme	immune	353:358	arg1	response					360:367	A balanced immune response	342:367	A balanced immune response	342:367	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	6	8	from	receptors	937:945	arg1	cells					957:961	immune cells	950:961	immune cells	950:961	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	8	9	theme	viral	1315:1319	arg1	infection					1321:1329	viral infection	1315:1329	viral infection	1315:1329	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	4	10	theme	innate	459:464	arg1	cells					473:477	innate immune cells	459:477	innate immune cells with the least tissue damage	459:506	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	9	11	theme	syndrome	1596:1603	arg1	causation					1532:1540	the causation	1528:1540	the causation of cytokine storms and systemic inflammatory response syndrome	1528:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	4	12	theme	balanced	344:351	arg1	response					360:367	A balanced immune response	342:367	A balanced immune response	342:367	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	5	13	dep	longer	522:527	arg1	system					540:545	the immune system	529:545	The longer the immune system	518:545	The longer the immune system takes to control the infection, the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host.
33843779	6	14	theme	Fc	934:935	arg1	receptors					937:945	Fc receptors	934:945	Fc receptors in immune cells	934:961	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	9	15	theme	antibodies	1514:1523	arg1	role					1506:1509	the role	1502:1509	the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome	1502:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	7	16	theme	rules	1052:1056	arg1	understanding					1031:1043	our understanding	1027:1043	our understanding of the rules governing the production of protective and nonprotective antibodies	1027:1124	The COVID-19 pandemic has provided a wealth of data bolstering our understanding of the rules governing the production of protective and nonprotective antibodies.
33843779	6	17	theme	effector	887:894	arg1	side					896:899	the effector side	883:899	the effector side	883:899	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	8	18	theme	mechanisms	1279:1288	arg1	knowledge					1252:1260	our knowledge	1248:1260	our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage	1248:1410	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	2	19	theme	fight	119:123	arg1	infections					125:134	fight infections	119:134	fight infections	119:134	Antibodies are a fundamental tool to fight infections but are intrinsically built as a double-edged sword.
33843779	6	20	theme	antibodies	739:748	arg1	quality					728:734	the quality	724:734	the quality of antibodies	724:748	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	3	21	theme	immune	300:305	arg1	cells					307:311	immune cells	300:311	immune cells	300:311	One side recognizes the microbial antigen, and the other gives a call to arms to fight infection by recruiting immune cells and triggering inflammation.
33843779	4	22	theme	invading	441:448	arg1	agent					450:454	the invading agent	437:454	the invading agent	437:454	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	5	23	with	process	641:647	arg1	consequences					672:683	potentially fatal consequences	654:683	potentially fatal consequences for the host	654:696	The longer the immune system takes to control the infection, the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host.
33843779	9	24	theme	great	1467:1471	arg1	deal					1473:1476	a great deal	1465:1476	a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases	1465:1660	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	6	25	theme	Fc	841:842	arg1	fractions					844:852	Fc fractions	841:852	Fc fractions	841:852	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	9	26	theme	response	1587:1594	arg1	syndrome					1596:1603	systemic inflammatory response syndrome	1565:1603	systemic inflammatory response syndrome	1565:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	9	27	theme	autoimmune	1642:1651	arg1	diseases					1653:1660	systemic autoimmune diseases	1633:1660	systemic autoimmune diseases	1633:1660	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	9	28	with	patients	1619:1626	arg1	diseases					1653:1660	systemic autoimmune diseases	1633:1660	systemic autoimmune diseases	1633:1660	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	3	29	theme	microbial	213:221	arg1	antigen					223:229	the microbial antigen	209:229	the microbial antigen	209:229	One side recognizes the microbial antigen, and the other gives a call to arms to fight infection by recruiting immune cells and triggering inflammation.
33843779	9	30	theme	role	1506:1509	arg1	comprehension					1485:1497	our comprehension	1481:1497	our comprehension	1481:1497	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	9	31	from	causation	1532:1540	arg1	comprehension					1485:1497	our comprehension	1481:1497	our comprehension	1481:1497	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	8	32	theme	viruses	1183:1189	arg1	role					1175:1178	the role	1171:1178	the role of viruses in triggering autoimmunity and inflammation	1171:1233	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	5	33	dep	higher	583:588	arg1	possibility					594:604	the possibility	590:604	the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host	579:696	The longer the immune system takes to control the infection, the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host.
33843779	4	34	theme	tissue	494:499	arg1	damage					501:506	the least tissue damage	484:506	the least tissue damage	484:506	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	5	35	theme	immune	533:538	arg1	system					540:545	the immune system	529:545	The longer the immune system	518:545	The longer the immune system takes to control the infection, the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host.
33843779	6	36	from	composition	826:836	arg1	fractions					844:852	Fc fractions	841:852	Fc fractions	841:852	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	7	37	theme	data	1011:1014	arg1	wealth					1001:1006	a wealth	999:1006	a wealth of data bolstering our understanding of the rules governing the production of protective and nonprotective antibodies	999:1124	The COVID-19 pandemic has provided a wealth of data bolstering our understanding of the rules governing the production of protective and nonprotective antibodies.
33843779	8	38	theme	progressive	1386:1396	arg1	damage					1405:1410	progressive tissue damage	1386:1410	progressive tissue damage	1386:1410	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	4	39	with	cells	473:477	arg1	damage					501:506	the least tissue damage	484:506	the least tissue damage	484:506	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	7	40	theme	protective	1086:1095	arg1	antibodies					1115:1124	protective and nonprotective antibodies	1086:1124	protective and nonprotective antibodies	1086:1124	The COVID-19 pandemic has provided a wealth of data bolstering our understanding of the rules governing the production of protective and nonprotective antibodies.
33843779	9	41	theme	COVID-19	1430:1437	arg1	infection					1439:1447	the COVID-19 infection	1426:1447	the COVID-19 infection	1426:1447	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	5	42	theme	fatal	666:670	arg1	consequences					672:683	potentially fatal consequences	654:683	potentially fatal consequences for the host	654:696	The longer the immune system takes to control the infection, the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host.
33843779	6	43	dep	quantity	714:721	arg1	addition					702:709	addition	702:709	addition	702:709	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	9	44	from	role	1506:1509	arg1	causation					1532:1540	the causation	1528:1540	the causation of cytokine storms and systemic inflammatory response syndrome	1528:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	4	45	theme	swift	419:423	arg1	disposal					425:432	a swift disposal	417:432	a swift disposal of the invading agent by innate immune cells with the least tissue damage	417:506	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	7	46	theme	nonprotective	1101:1113	arg1	antibodies					1115:1124	protective and nonprotective antibodies	1086:1124	protective and nonprotective antibodies	1086:1124	The COVID-19 pandemic has provided a wealth of data bolstering our understanding of the rules governing the production of protective and nonprotective antibodies.
33843779	8	47	theme	tissue	1398:1403	arg1	damage					1405:1410	progressive tissue damage	1386:1410	progressive tissue damage	1386:1410	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	8	48	theme	autoantibody	1343:1354	arg1	production					1356:1365	autoantibody production	1343:1365	autoantibody production	1343:1365	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	7	49	theme	COVID-19	968:975	arg1	pandemic					977:984	The COVID-19 pandemic	964:984	The COVID-19 pandemic	964:984	The COVID-19 pandemic has provided a wealth of data bolstering our understanding of the rules governing the production of protective and nonprotective antibodies.
33843779	4	50	theme	least	488:492	arg1	damage					501:506	the least tissue damage	484:506	the least tissue damage	484:506	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	5	51	theme	self-sustaining	612:626	arg1	process					641:647	a self-sustaining inflammatory process	610:647	a self-sustaining inflammatory process with potentially fatal consequences for the host	610:696	The longer the immune system takes to control the infection, the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host.
33843779	6	52	theme	N-glycan	817:824	arg1	composition					826:836	the N-glycan composition	813:836	the N-glycan composition in Fc fractions	813:852	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	9	53	theme	systemic	1565:1572	arg1	syndrome					1596:1603	systemic inflammatory response syndrome	1565:1603	systemic inflammatory response syndrome	1565:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	5	54	theme	inflammatory	628:639	arg1	process					641:647	a self-sustaining inflammatory process	610:647	a self-sustaining inflammatory process with potentially fatal consequences for the host	610:696	The longer the immune system takes to control the infection, the higher the possibility for a self-sustaining inflammatory process with potentially fatal consequences for the host.
33843779	2	55	theme	double-edged	169:180	arg1	Antibodies					82:91	Antibodies	82:91	Antibodies	82:91	Antibodies are a fundamental tool to fight infections but are intrinsically built as a double-edged sword.
33843779	2	55	theme	double-edged	169:180	arg1	sword					182:186	a double-edged sword	167:186	a double-edged sword	167:186	Antibodies are a fundamental tool to fight infections but are intrinsically built as a double-edged sword.
33843779	9	56	theme	cytokine	1545:1552	arg1	storms					1554:1559	cytokine storms	1545:1559	cytokine storms	1545:1559	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	4	57	theme	neutralizing	391:402	arg1	antibody					404:411	a potent neutralizing antibody	382:411	a potent neutralizing antibody	382:411	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	9	58	theme	storms	1554:1559	arg1	causation					1532:1540	the causation	1528:1540	the causation of cytokine storms and systemic inflammatory response syndrome	1528:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	4	59	theme	potent	384:389	arg1	antibody					404:411	a potent neutralizing antibody	382:411	a potent neutralizing antibody	382:411	A balanced immune response must combine a potent neutralizing antibody and a swift disposal of the invading agent by innate immune cells with the least tissue damage possible.
33843779	9	60	from	comprehension	1485:1497	arg1	causation					1532:1540	the causation	1528:1540	the causation of cytokine storms and systemic inflammatory response syndrome	1528:1603	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	8	61	theme	role	1175:1178	arg1	understanding					1154:1166	our understanding	1150:1166	our understanding of the role of viruses in triggering autoimmunity and inflammation	1150:1233	Also, it has broadened our understanding of the role of viruses in triggering autoimmunity and inflammation, and widened our knowledge of the different mechanisms that can be activated by viral infection and lead to autoantibody production, inflammation, and progressive tissue damage.
33843779	6	62	dep	help	858:861	arg1	tilt					863:866	tilt	863:866	tilt	863:866	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
33843779	2	63	theme	fundamental	99:109	arg1	Antibodies					82:91	Antibodies	82:91	Antibodies	82:91	Antibodies are a fundamental tool to fight infections but are intrinsically built as a double-edged sword.
33843779	2	63	theme	fundamental	99:109	arg1	tool					111:114	a fundamental tool	97:114	a fundamental tool to fight infections	97:134	Antibodies are a fundamental tool to fight infections but are intrinsically built as a double-edged sword.
33843779	7	64	theme	antibodies	1115:1124	arg1	production					1072:1081	the production	1068:1081	the production of protective and nonprotective antibodies	1068:1124	The COVID-19 pandemic has provided a wealth of data bolstering our understanding of the rules governing the production of protective and nonprotective antibodies.
33843779	9	65	theme	systemic	1633:1640	arg1	diseases					1653:1660	systemic autoimmune diseases	1633:1660	systemic autoimmune diseases	1633:1660	In addition, the COVID-19 infection has contributed a great deal to our comprehension of the role of antibodies in the causation of cytokine storms and systemic inflammatory response syndrome, also seen in patients with systemic autoimmune diseases.
33843779	6	66	theme	immune	950:955	arg1	cells					957:961	immune cells	950:961	immune cells	950:961	In addition to quantity, the quality of antibodies also matters, because posttranslational modifications altering the N-glycan composition in Fc fractions may help tilt the balance to the effector side, by modifying their affinity for Fc receptors in immune cells.
35244662	6	0	link	N-linked	1414:1421	arg1	glycopeptides					1423:1435	different N-linked glycopeptides	1404:1435	different N-linked glycopeptides	1404:1435	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	0	1	link	N-linked	96:103	arg1	glycopeptides					105:117	N-linked glycopeptides	96:117	N-linked glycopeptides	96:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	4	2	theme	N-linked	936:943	arg1	glycopeptides					945:957	274 N-linked glycopeptides	932:957	274 N-linked glycopeptides from 101 glycoproteins	932:980	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	3	theme	excellent	477:485	arg1	hydrophilicity					487:500	excellent hydrophilicity	477:500	excellent hydrophilicity	477:500	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	6	4	theme	GO	1348:1349	arg1	probe					1365:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	7	5	theme	diagnostic	1696:1705	arg1	methods					1707:1713	novel clinical diagnostic methods	1681:1713	novel clinical diagnostic methods	1681:1713	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	2	6	theme	MOFs-303-functionalized	285:307	arg1	MOF-303					334:340	GO@Fe3O4@MOF-303	325:340	GO@Fe3O4@MOF-303	325:340	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	6	theme	MOFs-303-functionalized	285:307	arg1	probe					318:322	a hydrophilic MOFs-303-functionalized magnetic probe	271:322	a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303)	271:341	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	5	7	theme	down-regulated	1214:1227	arg1	glycoproteins					1235:1247	4 up-regulated and 19 down-regulated serum glycoproteins	1192:1247	4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients	1192:1263	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	7	8	theme	novel	1681:1685	arg1	methods					1707:1713	novel clinical diagnostic methods	1681:1713	novel clinical diagnostic methods	1681:1713	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	3	9	theme	protein/HRP	661:671	arg1	w/w/w					699:703	w/w/w	699:703	w/w/w	699:703	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	9	theme	protein/HRP	661:671	arg1	protein					673:679	HRP digests/BSA protein/HRP protein	645:679	HRP digests/BSA protein/HRP protein	645:679	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	9	theme	protein/HRP	661:671	arg1	1 					682:683	1 	682:683	1 	682:683	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	5	10	theme	clustering	1285:1294	arg1	heatmap					1296:1302	the hierarchical clustering heatmap	1268:1302	the hierarchical clustering heatmap	1268:1302	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	3	11	theme	porous	515:520	arg1	structure					522:530	suitable porous structure	506:530	suitable porous structure	506:530	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	6	12	theme	N-linked	1414:1421	arg1	glycopeptides					1423:1435	different N-linked glycopeptides	1404:1435	different N-linked glycopeptides	1404:1435	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	2	13	theme	GO	325:326	arg1	MOF-303					334:340	GO@Fe3O4@MOF-303	325:340	GO@Fe3O4@MOF-303	325:340	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	13	theme	GO	325:326	arg1	probe					318:322	a hydrophilic MOFs-303-functionalized magnetic probe	271:322	a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303)	271:341	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	7	14	theme	effective	1525:1533	arg1	capture					1535:1541	the highly effective capture	1514:1541	the highly effective capture of N-linked glycopeptides	1514:1567	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	4	15	gly	glycoproteins	968:980	arg1	glycoproteins					968:980	101 glycoproteins	964:980	101 glycoproteins	964:980	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	16	theme	GO	537:538	arg1	probe					554:558	the GO@Fe3O4@MOF-303 probe	533:558	the GO@Fe3O4@MOF-303 probe	533:558	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	4	17	theme	healthy	1011:1017	arg1	controls					1019:1026	healthy controls	1011:1026	healthy controls	1011:1026	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	5	18	gly	glycoproteins	1235:1247	arg1	glycoproteins					1235:1247	4 up-regulated and 19 down-regulated serum glycoproteins	1192:1247	4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients	1192:1263	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	6	19	gly	glycopeptides	1423:1435	arg2	glycopeptides					1423:1435	different N-linked glycopeptides	1404:1435	different N-linked glycopeptides	1404:1435	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	3	20	theme	excellent	748:756	arg1	reusability					758:768	excellent reusability	748:768	excellent reusability in the capture of standard N-linked glycopeptides	748:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	21	theme	binding	714:720	arg1	capacity					722:729	a high binding capacity	707:729	a high binding capacity (200 mg g-1)	707:742	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	21	theme	binding	714:720	arg1	g-1					739:741	200 mg g-1	732:741	200 mg g-1	732:741	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	4	22	from	glycoproteins	968:980	arg1	glycopeptides					945:957	274 N-linked glycopeptides	932:957	274 N-linked glycopeptides from 101 glycoproteins	932:980	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	23	gly	glycopeptides	806:818	arg2	glycopeptides					806:818	standard N-linked glycopeptides	788:818	standard N-linked glycopeptides	788:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	24	theme	ultralow	572:579	arg1	fmol					602:605	0.1 fmol μL-1	598:610	0.1 fmol μL-1	598:610	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	24	theme	ultralow	572:579	arg1	limit					591:595	an ultralow detection limit	569:595	an ultralow detection limit (0.1 fmol μL-1)	569:611	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	6	25	theme	clinical	1448:1455	arg1	samples					1457:1463	complex clinical samples	1440:1463	complex clinical samples	1440:1463	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	1	26	theme	clinical	165:172	arg1	samples					182:188	clinical complex samples	165:188	clinical complex samples	165:188	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	4	27	theme	N-linked	1050:1057	arg1	glycopeptides					1059:1071	265 N-linked glycopeptides	1046:1071	265 N-linked glycopeptides from 102 glycoproteins	1046:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	28	theme	mg	736:737	arg1	capacity					722:729	a high binding capacity	707:729	a high binding capacity (200 mg g-1)	707:742	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	28	theme	mg	736:737	arg1	g-1					739:741	200 mg g-1	732:741	200 mg g-1	732:741	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	7	29	link	N-linked	1546:1553	arg1	glycopeptides					1555:1567	N-linked glycopeptides	1546:1567	N-linked glycopeptides	1546:1567	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	4	30	theme	@	844:844	arg1	probe					859:863	the GO@Fe3O4@MOF-303 probe	838:863	the GO@Fe3O4@MOF-303 probe	838:863	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	0	31	theme	N-linked	96:103	arg1	glycopeptides					105:117	N-linked glycopeptides	96:117	N-linked glycopeptides	96:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	3	32	theme	glycopeptides	806:818	arg1	capture					777:783	the capture	773:783	the capture of standard N-linked glycopeptides	773:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	1	33	theme	glycoproteomics	146:160	arg1	analysis					134:141	The effective analysis	120:141	The effective analysis of glycoproteomics in clinical complex samples	120:188	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	4	34	theme	@	850:850	arg1	probe					859:863	the GO@Fe3O4@MOF-303 probe	838:863	the GO@Fe3O4@MOF-303 probe	838:863	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	35	link	N-linked	797:804	arg1	glycopeptides					806:818	standard N-linked glycopeptides	788:818	standard N-linked glycopeptides	788:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	36	theme	standard	788:795	arg1	glycopeptides					806:818	standard N-linked glycopeptides	788:818	standard N-linked glycopeptides	788:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	37	from	limit	591:595	arg1	capture					777:783	the capture	773:783	the capture of standard N-linked glycopeptides	773:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	38	theme	strong	418:423	arg1	property					434:441	strong magnetic property	418:441	strong magnetic property	418:441	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	39	dep	effect	637:642	arg1	w/w/w					699:703	w/w/w	699:703	w/w/w	699:703	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	39	dep	effect	637:642	arg1	protein					673:679	HRP digests/BSA protein/HRP protein	645:679	HRP digests/BSA protein/HRP protein	645:679	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	39	dep	effect	637:642	arg1	1 					682:683	1 	682:683	1 	682:683	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	0	40	gly	glycopeptides	105:117	arg2	glycopeptides					105:117	N-linked glycopeptides	96:117	N-linked glycopeptides	96:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	7	41	theme	N-linked	1546:1553	arg1	glycopeptides					1555:1567	N-linked glycopeptides	1546:1567	N-linked glycopeptides	1546:1567	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	5	42	from	glycoproteins	1235:1247	arg1	patients					1256:1263	HCC patients	1252:1263	HCC patients	1252:1263	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	4	43	link	N-linked	1050:1057	arg1	glycopeptides					1059:1071	265 N-linked glycopeptides	1046:1071	265 N-linked glycopeptides from 102 glycoproteins	1046:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	0	44	theme	hydrophilic	8:18	arg1	probe					53:57	A novel hydrophilic MOFs-303-functionalized magnetic probe	0:57	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides	0:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	4	45	gly	glycoproteins	1082:1094	arg1	glycoproteins					1082:1094	102 glycoproteins	1078:1094	102 glycoproteins	1078:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	46	theme	suitable	506:513	arg1	structure					522:530	suitable porous structure	506:530	suitable porous structure	506:530	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	47	theme	large	444:448	arg1	g-1					471:473	845 m2 g-1	464:473	845 m2 g-1	464:473	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	47	theme	large	444:448	arg1	area					458:461	large surface area	444:461	large surface area (845 m2 g-1)	444:474	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	0	48	theme	magnetic	44:51	arg1	probe					53:57	A novel hydrophilic MOFs-303-functionalized magnetic probe	0:57	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides	0:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	6	49	contain	has	1371:1373	arg1	probe					1365:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	6	49	contain	has	1371:1373	arg2	potential					1381:1389	great potential	1375:1389	great potential	1375:1389	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	4	50	from	glycoproteins	1082:1094	arg1	glycopeptides					1059:1071	265 N-linked glycopeptides	1046:1071	265 N-linked glycopeptides from 102 glycoproteins	1046:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	4	50	from	glycoproteins	1082:1094	arg1	total					1037:1041	a total	1035:1041	a total of 265 N-linked glycopeptides from 102 glycoproteins	1035:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	4	51	theme	practical	902:910	arg1	applications					912:923	practical applications	902:923	practical applications	902:923	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	2	52	link	N-linked	381:388	arg1	glycopeptides					390:402	profile N-linked glycopeptides	373:402	profile N-linked glycopeptides	373:402	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	4	53	from	performance	887:897	arg1	applications					912:923	practical applications	902:923	practical applications	902:923	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	4	54	theme	hepatocellular	1133:1146	arg1	HCC					1159:1161	HCC	1159:1161	HCC	1159:1161	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	4	54	theme	hepatocellular	1133:1146	arg1	carcinoma					1148:1156	hepatocellular carcinoma	1133:1156	hepatocellular carcinoma (HCC)	1133:1162	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	1	55	dep	diagnosis	221:229	arg1	the					217:219	the	217:219	the	217:219	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	5	56	theme	hierarchical	1272:1283	arg1	heatmap					1296:1302	the hierarchical clustering heatmap	1268:1302	the hierarchical clustering heatmap	1268:1302	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	2	57	theme	@	333:333	arg1	MOF-303					334:340	GO@Fe3O4@MOF-303	325:340	GO@Fe3O4@MOF-303	325:340	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	57	theme	@	333:333	arg1	probe					318:322	a hydrophilic MOFs-303-functionalized magnetic probe	271:322	a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303)	271:341	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	6	58	theme	@	1356:1356	arg1	probe					1365:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	1	59	theme	diseases	246:253	arg1	therapy					235:241	therapy	235:241	therapy	235:241	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	1	59	theme	diseases	246:253	arg1	diagnosis					221:229	diagnosis	221:229	diagnosis	221:229	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	7	60	gly	glycopeptides	1555:1567	arg2	glycopeptides					1555:1567	N-linked glycopeptides	1546:1567	N-linked glycopeptides	1546:1567	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	7	61	theme	methods	1707:1713	arg1	development					1666:1676	the development	1662:1676	the development of novel clinical diagnostic methods	1662:1713	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	3	62	theme	digests/BSA	649:659	arg1	w/w/w					699:703	w/w/w	699:703	w/w/w	699:703	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	62	theme	digests/BSA	649:659	arg1	protein					673:679	HRP digests/BSA protein/HRP protein	645:679	HRP digests/BSA protein/HRP protein	645:679	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	62	theme	digests/BSA	649:659	arg1	1 					682:683	1 	682:683	1 	682:683	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	5	63	theme	HCC	1252:1254	arg1	patients					1256:1263	HCC patients	1252:1263	HCC patients	1252:1263	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	6	64	theme	@	1350:1350	arg1	probe					1365:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	7	65	theme	clinical	1687:1694	arg1	methods					1707:1713	novel clinical diagnostic methods	1681:1713	novel clinical diagnostic methods	1681:1713	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	2	66	theme	magnetic	309:316	arg1	MOF-303					334:340	GO@Fe3O4@MOF-303	325:340	GO@Fe3O4@MOF-303	325:340	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	66	theme	magnetic	309:316	arg1	probe					318:322	a hydrophilic MOFs-303-functionalized magnetic probe	271:322	a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303)	271:341	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	6	67	theme	reusable	1339:1346	arg1	probe					1365:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	3	68	theme	@	539:539	arg1	probe					554:558	the GO@Fe3O4@MOF-303 probe	533:558	the GO@Fe3O4@MOF-303 probe	533:558	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	2	69	theme	hydrophilic	273:283	arg1	MOF-303					334:340	GO@Fe3O4@MOF-303	325:340	GO@Fe3O4@MOF-303	325:340	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	69	theme	hydrophilic	273:283	arg1	probe					318:322	a hydrophilic MOFs-303-functionalized magnetic probe	271:322	a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303)	271:341	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	4	70	from	total	1037:1041	arg1	glycoproteins					1082:1094	102 glycoproteins	1078:1094	102 glycoproteins	1078:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	2	71	theme	@	327:327	arg1	MOF-303					334:340	GO@Fe3O4@MOF-303	325:340	GO@Fe3O4@MOF-303	325:340	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	71	theme	@	327:327	arg1	probe					318:322	a hydrophilic MOFs-303-functionalized magnetic probe	271:322	a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303)	271:341	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	6	72	theme	different	1404:1412	arg1	glycopeptides					1423:1435	different N-linked glycopeptides	1404:1435	different N-linked glycopeptides	1404:1435	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	1	73	theme	vital	196:200	arg1	importance					202:211	vital importance	196:211	vital importance	196:211	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	4	74	link	N-linked	936:943	arg1	glycopeptides					945:957	274 N-linked glycopeptides	932:957	274 N-linked glycopeptides from 101 glycoproteins	932:980	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	75	theme	Fe3O4	540:544	arg1	probe					554:558	the GO@Fe3O4@MOF-303 probe	533:558	the GO@Fe3O4@MOF-303 probe	533:558	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	76	from	reusability	758:768	arg1	capture					777:783	the capture	773:783	the capture of standard N-linked glycopeptides	773:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	77	from	effect	637:642	arg1	capture					777:783	the capture	773:783	the capture of standard N-linked glycopeptides	773:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	6	78	theme	great	1375:1379	arg1	potential					1381:1389	great potential	1375:1389	great potential	1375:1389	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	4	79	with	serum	1115:1119	arg1	HCC					1159:1161	HCC	1159:1161	HCC	1159:1161	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	4	79	with	serum	1115:1119	arg1	carcinoma					1148:1156	hepatocellular carcinoma	1133:1156	hepatocellular carcinoma (HCC)	1133:1162	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	7	80	theme	novel	1498:1502	arg1	probe					1504:1508	a novel probe	1496:1508	a novel probe for the highly effective capture of N-linked glycopeptides	1496:1567	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	3	81	theme	@	545:545	arg1	probe					554:558	the GO@Fe3O4@MOF-303 probe	533:558	the GO@Fe3O4@MOF-303 probe	533:558	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	1	82	theme	effective	124:132	arg1	analysis					134:141	The effective analysis	120:141	The effective analysis of glycoproteomics in clinical complex samples	120:188	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	3	83	theme	size-exclusion	622:635	arg1	effect					637:642	perfect size-exclusion effect	614:642	perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w)	614:704	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	4	84	gly	glycopeptides	945:957	arg1	glycoproteins					968:980	101 glycoproteins	964:980	101 glycoproteins	964:980	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	4	84	gly	glycopeptides	945:957	arg2	glycopeptides					945:957	274 N-linked glycopeptides	932:957	274 N-linked glycopeptides from 101 glycoproteins	932:980	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	85	theme	detection	581:589	arg1	fmol					602:605	0.1 fmol μL-1	598:610	0.1 fmol μL-1	598:610	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	85	theme	detection	581:589	arg1	limit					591:595	an ultralow detection limit	569:595	an ultralow detection limit (0.1 fmol μL-1)	569:611	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	0	86	theme	efficient	74:82	arg1	analysis					84:91	the highly efficient analysis	63:91	the highly efficient analysis of N-linked glycopeptides	63:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	7	87	theme	HCC	1632:1634	arg1	mechanism					1619:1627	the mechanism	1615:1627	the mechanism of HCC	1615:1634	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	4	88	theme	glycopeptides	1059:1071	arg1	total					1037:1041	a total	1035:1041	a total of 265 N-linked glycopeptides from 102 glycoproteins	1035:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	89	theme	high	709:712	arg1	capacity					722:729	a high binding capacity	707:729	a high binding capacity (200 mg g-1)	707:742	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	89	theme	high	709:712	arg1	g-1					739:741	200 mg g-1	732:741	200 mg g-1	732:741	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	6	90	theme	complex	1440:1446	arg1	samples					1457:1463	complex clinical samples	1440:1463	complex clinical samples	1440:1463	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
35244662	1	91	theme	complex	174:180	arg1	samples					182:188	clinical complex samples	165:188	clinical complex samples	165:188	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	0	92	theme	glycopeptides	105:117	arg1	analysis					84:91	the highly efficient analysis	63:91	the highly efficient analysis of N-linked glycopeptides	63:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	1	93	from	samples	182:188	arg1	analysis					134:141	The effective analysis	120:141	The effective analysis of glycoproteomics in clinical complex samples	120:188	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	4	94	theme	GO	842:843	arg1	probe					859:863	the GO@Fe3O4@MOF-303 probe	838:863	the GO@Fe3O4@MOF-303 probe	838:863	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	5	95	theme	up-regulated	1194:1205	arg1	glycoproteins					1235:1247	4 up-regulated and 19 down-regulated serum glycoproteins	1192:1247	4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients	1192:1263	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	3	96	theme	m2	468:469	arg1	g-1					471:473	845 m2 g-1	464:473	845 m2 g-1	464:473	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	96	theme	m2	468:469	arg1	area					458:461	large surface area	444:461	large surface area (845 m2 g-1)	444:474	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	4	97	theme	Fe3O4	845:849	arg1	probe					859:863	the GO@Fe3O4@MOF-303 probe	838:863	the GO@Fe3O4@MOF-303 probe	838:863	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	2	98	gly	glycopeptides	390:402	arg2	glycopeptides					390:402	profile N-linked glycopeptides	373:402	profile N-linked glycopeptides	373:402	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	99	theme	N-linked	381:388	arg1	glycopeptides					390:402	profile N-linked glycopeptides	373:402	profile N-linked glycopeptides	373:402	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	0	100	theme	novel	2:6	arg1	probe					53:57	A novel hydrophilic MOFs-303-functionalized magnetic probe	0:57	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides	0:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	1	101	from	glycoproteomics	146:160	arg1	samples					182:188	clinical complex samples	165:188	clinical complex samples	165:188	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	3	102	theme	N-linked	797:804	arg1	glycopeptides					806:818	standard N-linked glycopeptides	788:818	standard N-linked glycopeptides	788:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	103	theme	magnetic	425:432	arg1	property					434:441	strong magnetic property	418:441	strong magnetic property	418:441	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	0	104	theme	MOFs-303-functionalized	20:42	arg1	probe					53:57	A novel hydrophilic MOFs-303-functionalized magnetic probe	0:57	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides	0:117	A novel hydrophilic MOFs-303-functionalized magnetic probe for the highly efficient analysis of N-linked glycopeptides.
35244662	1	105	from	analysis	134:141	arg1	samples					182:188	clinical complex samples	165:188	clinical complex samples	165:188	The effective analysis of glycoproteomics in clinical complex samples is of vital importance for the diagnosis and therapy of diseases.
35244662	7	106	theme	glycopeptides	1555:1567	arg1	capture					1535:1541	the highly effective capture	1514:1541	the highly effective capture of N-linked glycopeptides	1514:1567	This study not only developed a novel probe for the highly effective capture of N-linked glycopeptides but also contributed to further understanding the mechanism of HCC and provides guidance for the development of novel clinical diagnostic methods.
35244662	4	107	theme	remarkable	876:885	arg1	performance					887:897	remarkable performance	876:897	remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC)	876:1162	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	108	theme	surface	450:456	arg1	g-1					471:473	845 m2 g-1	464:473	845 m2 g-1	464:473	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	108	theme	surface	450:456	arg1	area					458:461	large surface area	444:461	large surface area (845 m2 g-1)	444:474	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	109	dep	protein	673:679	arg1	 1000					692:696	 1000	692:696	 1000	692:696	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	109	dep	protein	673:679	arg1	 1000 					685:690	 1000 	685:690	 1000 	685:690	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	5	110	dep	down-regulated	1214:1227	arg1	19					1211:1212	19	1211:1212	19	1211:1212	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	3	111	theme	perfect	614:620	arg1	effect					637:642	perfect size-exclusion effect	614:642	perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w)	614:704	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	4	112	gly	glycopeptides	1059:1071	arg1	glycoproteins					1082:1094	102 glycoproteins	1078:1094	102 glycoproteins	1078:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	4	112	gly	glycopeptides	1059:1071	arg2	glycopeptides					1059:1071	265 N-linked glycopeptides	1046:1071	265 N-linked glycopeptides from 102 glycoproteins	1046:1094	More excitingly, the GO@Fe3O4@MOF-303 probe also shows remarkable performance in practical applications, where 274 N-linked glycopeptides from 101 glycoproteins were identified in total for healthy controls, while a total of 265 N-linked glycopeptides from 102 glycoproteins were identified in serum (1 μL) with hepatocellular carcinoma (HCC).
35244662	3	113	from	capacity	722:729	arg1	capture					777:783	the capture	773:783	the capture of standard N-linked glycopeptides	773:818	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	5	114	theme	serum	1229:1233	arg1	glycoproteins					1235:1247	4 up-regulated and 19 down-regulated serum glycoproteins	1192:1247	4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients	1192:1263	In addition, we discovered 4 up-regulated and 19 down-regulated serum glycoproteins in HCC patients by the hierarchical clustering heatmap.
35244662	2	115	theme	Fe3O4	328:332	arg1	MOF-303					334:340	GO@Fe3O4@MOF-303	325:340	GO@Fe3O4@MOF-303	325:340	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	2	115	theme	Fe3O4	328:332	arg1	probe					318:322	a hydrophilic MOFs-303-functionalized magnetic probe	271:322	a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303)	271:341	In this study, a hydrophilic MOFs-303-functionalized magnetic probe (GO@Fe3O4@MOF-303) is designed and fabricated to profile N-linked glycopeptides.
35244662	3	116	theme	HRP	645:647	arg1	w/w/w					699:703	w/w/w	699:703	w/w/w	699:703	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	116	theme	HRP	645:647	arg1	protein					673:679	HRP digests/BSA protein/HRP protein	645:679	HRP digests/BSA protein/HRP protein	645:679	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	3	116	theme	HRP	645:647	arg1	1 					682:683	1 	682:683	1 	682:683	Owing to its strong magnetic property, large surface area (845 m2 g-1), excellent hydrophilicity and suitable porous structure, the GO@Fe3O4@MOF-303 probe exhibits an ultralow detection limit (0.1 fmol μL-1), perfect size-exclusion effect (HRP digests/BSA protein/HRP protein, 1 : 1000 : 1000, w/w/w), a high binding capacity (200 mg g-1) and excellent reusability in the capture of standard N-linked glycopeptides.
35244662	6	117	theme	Fe3O4	1351:1355	arg1	probe					1365:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	the reusable GO@Fe3O4@MOF-303 probe	1335:1369	All results demonstrated that the reusable GO@Fe3O4@MOF-303 probe has great potential in profiling different N-linked glycopeptides in complex clinical samples.
37067908	6	0	theme	percent	1201:1207	arg1	precision					1113:1121	precision	1113:1121	precision	1113:1121	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	0	theme	percent	1201:1207	arg1	specificity					1066:1076	specificity	1066:1076	specificity	1066:1076	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	0	theme	percent	1201:1207	arg1	sensitivity					1053:1063	sensitivity	1053:1063	sensitivity	1053:1063	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	0	theme	percent	1201:1207	arg1	accuracy					1128:1135	accuracy	1128:1135	accuracy	1128:1135	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	0	theme	percent	1201:1207	arg1	Coefficient					1100:1110	Matthews Correlation Coefficient	1079:1110	Matthews Correlation Coefficient	1079:1110	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	1	theme	test	1250:1253	arg1	set					1255:1257	a benchmark independent test set	1226:1257	a benchmark independent test set	1226:1257	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	1	2	theme	important	158:166	arg1	mechanism					187:195	an important post-translational mechanism	155:195	an important post-translational mechanism in Homo sapiens	155:211	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	1	2	theme	important	158:166	arg1	glycosylation					138:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	2	3	from	sequon	301:306	arg1	sequences					322:330	amino acid sequences	311:330	amino acid sequences	311:330	It occurs at the N-X-[S/T] sequon in amino acid sequences, where X can be any amino acid except proline.
37067908	5	4	theme	deep	881:884	arg1	approach					901:908	a deep learning-based approach	879:908	a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM)	879:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	6	5	theme	independent	1238:1248	arg1	set					1255:1257	a benchmark independent test set	1226:1257	a benchmark independent test set	1226:1257	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	1	6	theme	post-translational	168:185	arg1	mechanism					187:195	an important post-translational mechanism	155:195	an important post-translational mechanism in Homo sapiens	155:211	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	1	6	theme	post-translational	168:185	arg1	glycosylation					138:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	0	7	theme	protein	97:103	arg1	model					114:118	a pre-trained protein language model	83:118	a pre-trained protein language model	83:118	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	4	8	from	models	840:845	arg1	information					806:816	the distilled information	792:816	the distilled information from protein language models	792:845	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	6	9	theme	benchmark	1228:1236	arg1	set					1255:1257	a benchmark independent test set	1226:1257	a benchmark independent test set	1226:1257	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	5	10	theme	language	1011:1018	arg1	pLM					1027:1029	pLM	1027:1029	pLM	1027:1029	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	10	theme	language	1011:1018	arg1	model					1020:1024	a pre-trained protein language model	989:1024	a pre-trained protein language model (pLM)	989:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	6	11	theme	percent	1161:1167	arg1	precision					1113:1121	precision	1113:1121	precision	1113:1121	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	11	theme	percent	1161:1167	arg1	specificity					1066:1076	specificity	1066:1076	specificity	1066:1076	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	11	theme	percent	1161:1167	arg1	sensitivity					1053:1063	sensitivity	1053:1063	sensitivity	1053:1063	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	11	theme	percent	1161:1167	arg1	accuracy					1128:1135	accuracy	1128:1135	accuracy	1128:1135	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	11	theme	percent	1161:1167	arg1	Coefficient					1100:1110	Matthews Correlation Coefficient	1079:1110	Matthews Correlation Coefficient	1079:1110	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	4	12	from	problem	650:656	arg1	regard					539:544	that regard	534:544	that regard	534:544	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	6	13	theme	75.74	1195:1199	arg1	percent					1201:1207	75.74 percent	1195:1207	75.74 percent	1195:1207	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	0	14	theme	pre-trained	85:95	arg1	model					114:118	a pre-trained protein language model	83:118	a pre-trained protein language model	83:118	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	3	15	theme	protein	508:514	arg1	glycosylation					516:528	protein glycosylation	508:528	protein glycosylation	508:528	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	4	16	gly	glycosylation	584:596	arg2	sites					598:602	N-linked glycosylation sites	575:602	N-linked glycosylation sites confined to N-X-[S/T] sequons	575:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	2	17	theme	N-X-[S/T	291:298	arg1	sequon					301:306	the N-X-[S/T] sequon	287:306	the N-X-[S/T] sequon in amino acid sequences, where X can be any amino acid except proline	287:376	It occurs at the N-X-[S/T] sequon in amino acid sequences, where X can be any amino acid except proline.
37067908	4	18	theme	computational	547:559	arg1	problem					650:656	an important problem	637:656	an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models	637:845	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	4	18	theme	computational	547:559	arg1	prediction					561:570	computational prediction	547:570	computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons	547:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	5	19	gly	glycosylated	930:941	arg1	sites					943:947	N-linked glycosylated sites	921:947	N-linked glycosylated sites	921:947	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	7	20	theme	glycosylation	1353:1365	arg1	sites					1367:1371	N-linked glycosylation sites	1344:1371	N-linked glycosylation sites confined to the N-X-[S/T] sequon	1344:1404	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	4	21	theme	existing	706:713	arg1	methods					715:721	the existing methods	702:721	the existing methods	702:721	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	3	22	theme	sufficient	481:490	arg1	sequon					451:456	the N-X-[S/T] sequon	437:456	the N-X-[S/T] sequon	437:456	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	3	22	theme	sufficient	481:490	arg1	determinant					492:502	a necessary but not sufficient determinant	461:502	a necessary but not sufficient determinant for protein glycosylation	461:528	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	3	23	theme	N-X-[S/T	396:403	arg1	sequons					406:412	not all N-X-[S/T] sequons	388:412	not all N-X-[S/T] sequons	388:412	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	0	24	theme	language	105:112	arg1	model					114:118	a pre-trained protein language model	83:118	a pre-trained protein language model	83:118	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	7	25	gly	glycosylation	1353:1365	arg2	sites					1367:1371	N-linked glycosylation sites	1344:1371	N-linked glycosylation sites confined to the N-X-[S/T] sequon	1344:1404	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	6	26	theme	percent	1146:1152	arg1	precision					1113:1121	precision	1113:1121	precision	1113:1121	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	26	theme	percent	1146:1152	arg1	specificity					1066:1076	specificity	1066:1076	specificity	1066:1076	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	26	theme	percent	1146:1152	arg1	sensitivity					1053:1063	sensitivity	1053:1063	sensitivity	1053:1063	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	26	theme	percent	1146:1152	arg1	accuracy					1128:1135	accuracy	1128:1135	accuracy	1128:1135	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	26	theme	percent	1146:1152	arg1	Coefficient					1100:1110	Matthews Correlation Coefficient	1079:1110	Matthews Correlation Coefficient	1079:1110	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	7	27	theme	N-linked	1344:1351	arg1	sites					1367:1371	N-linked glycosylation sites	1344:1371	N-linked glycosylation sites confined to the N-X-[S/T] sequon	1344:1404	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	4	28	theme	language	831:838	arg1	models					840:845	protein language models	823:845	protein language models	823:845	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	2	29	theme	acid	317:320	arg1	sequences					322:330	amino acid sequences	311:330	amino acid sequences	311:330	It occurs at the N-X-[S/T] sequon in amino acid sequences, where X can be any amino acid except proline.
37067908	1	30	theme	Homo	200:203	arg1	sapiens					205:211	Homo sapiens	200:211	Homo sapiens	200:211	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	4	31	theme	protein	823:829	arg1	models					840:845	protein language models	823:845	protein language models	823:845	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	0	32	link	N-linked	32:39	arg1	sites					55:59	human N-linked glycosylation sites	26:59	human N-linked glycosylation sites	26:59	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	2	33	theme	amino	311:315	arg1	sequences					322:330	amino acid sequences	311:330	amino acid sequences	311:330	It occurs at the N-X-[S/T] sequon in amino acid sequences, where X can be any amino acid except proline.
37067908	7	34	theme	N-X-[S/T	1389:1396	arg1	sequon					1399:1404	the N-X-[S/T] sequon	1385:1404	the N-X-[S/T] sequon	1385:1404	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	4	35	theme	sets	772:775	arg1	creation					751:758	the creation	747:758	the creation of negative sets	747:775	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	6	36	theme	percent	1182:1188	arg1	precision					1113:1121	precision	1113:1121	precision	1113:1121	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	36	theme	percent	1182:1188	arg1	specificity					1066:1076	specificity	1066:1076	specificity	1066:1076	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	36	theme	percent	1182:1188	arg1	sensitivity					1053:1063	sensitivity	1053:1063	sensitivity	1053:1063	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	36	theme	percent	1182:1188	arg1	accuracy					1128:1135	accuracy	1128:1135	accuracy	1128:1135	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	6	36	theme	percent	1182:1188	arg1	Coefficient					1100:1110	Matthews Correlation Coefficient	1079:1110	Matthews Correlation Coefficient	1079:1110	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	5	37	theme	N-linked	921:928	arg1	sites					943:947	N-linked glycosylated sites	921:947	N-linked glycosylated sites	921:947	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	38	theme	glycosylated	930:941	arg1	sites					943:947	N-linked glycosylated sites	921:947	N-linked glycosylated sites	921:947	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	39	theme	human	952:956	arg1	proteins					958:965	human proteins	952:965	human proteins using embeddings from a pre-trained protein language model (pLM)	952:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	3	40	theme	necessary	463:471	arg1	sequon					451:456	the N-X-[S/T] sequon	437:456	the N-X-[S/T] sequon	437:456	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	3	40	theme	necessary	463:471	arg1	determinant					492:502	a necessary but not sufficient determinant	461:502	a necessary but not sufficient determinant for protein glycosylation	461:528	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	6	41	theme	Correlation	1088:1098	arg1	Coefficient					1100:1110	Matthews Correlation Coefficient	1079:1110	Matthews Correlation Coefficient	1079:1110	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	0	42	theme	N-linked	32:39	arg1	sites					55:59	human N-linked glycosylation sites	26:59	human N-linked glycosylation sites	26:59	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	4	43	theme	important	640:648	arg1	problem					650:656	an important problem	637:656	an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models	637:845	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	4	43	theme	important	640:648	arg1	prediction					561:570	computational prediction	547:570	computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons	547:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	5	44	theme	pre-trained	991:1001	arg1	pLM					1027:1029	pLM	1027:1029	pLM	1027:1029	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	44	theme	pre-trained	991:1001	arg1	model					1020:1024	a pre-trained protein language model	989:1024	a pre-trained protein language model (pLM)	989:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	1	45	theme	essential	222:230	arg1	roles					232:236	essential roles	222:236	essential roles	222:236	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	0	46	theme	human	26:30	arg1	sites					55:59	human N-linked glycosylation sites	26:59	human N-linked glycosylation sites	26:59	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	4	47	link	N-linked	575:582	arg1	sites					598:602	N-linked glycosylation sites	575:602	N-linked glycosylation sites confined to N-X-[S/T] sequons	575:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	4	48	theme	sites	598:602	arg1	problem					650:656	an important problem	637:656	an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models	637:845	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	4	48	theme	sites	598:602	arg1	prediction					561:570	computational prediction	547:570	computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons	547:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	4	49	theme	N-X-[S/T	616:623	arg1	sequons					626:632	N-X-[S/T] sequons	616:632	N-X-[S/T] sequons	616:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	0	50	theme	sites	55:59	arg1	prediction					12:21	prediction	12:21	prediction of human N-linked glycosylation sites	12:59	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	1	51	link	N-linked	129:136	arg1	mechanism					187:195	an important post-translational mechanism	155:195	an important post-translational mechanism in Homo sapiens	155:211	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	1	51	link	N-linked	129:136	arg1	glycosylation					138:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	4	52	theme	distilled	796:804	arg1	information					806:816	the distilled information	792:816	the distilled information from protein language models	792:845	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	0	53	theme	glycosylation	41:53	arg1	sites					55:59	human N-linked glycosylation sites	26:59	human N-linked glycosylation sites	26:59	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	3	54	theme	N-X-[S/T	441:448	arg1	sequon					451:456	the N-X-[S/T] sequon	437:456	the N-X-[S/T] sequon	437:456	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	3	54	theme	N-X-[S/T	441:448	arg1	determinant					492:502	a necessary but not sufficient determinant	461:502	a necessary but not sufficient determinant for protein glycosylation	461:528	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	0	55	from	model	114:118	arg1	embeddings					67:76	embeddings	67:76	embeddings from a pre-trained protein language model	67:118	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	3	56	gly	glycosylated	418:429	arg1	sequons					406:412	not all N-X-[S/T] sequons	388:412	not all N-X-[S/T] sequons	388:412	However, not all N-X-[S/T] sequons are glycosylated, thus the N-X-[S/T] sequon is a necessary but not sufficient determinant for protein glycosylation.
37067908	7	57	theme	robust	1307:1312	arg1	tool					1328:1331	a robust computational tool	1305:1331	a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon	1305:1404	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	7	57	theme	robust	1307:1312	arg1	LMNglyPred					1291:1300	LMNglyPred	1291:1300	LMNglyPred	1291:1300	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	1	58	theme	Protein	121:127	arg1	mechanism					187:195	an important post-translational mechanism	155:195	an important post-translational mechanism in Homo sapiens	155:211	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	1	58	theme	Protein	121:127	arg1	glycosylation					138:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	4	59	theme	negative	763:770	arg1	sets					772:775	negative sets	763:775	negative sets	763:775	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	7	60	theme	computational	1314:1326	arg1	tool					1328:1331	a robust computational tool	1305:1331	a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon	1305:1404	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	7	60	theme	computational	1314:1326	arg1	LMNglyPred					1291:1300	LMNglyPred	1291:1300	LMNglyPred	1291:1300	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	5	61	dep	developed	857:865	arg1	LMNglyPred					867:876	LMNglyPred	867:876	LMNglyPred	867:876	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	62	theme	protein	1003:1009	arg1	pLM					1027:1029	pLM	1027:1029	pLM	1027:1029	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	62	theme	protein	1003:1009	arg1	model					1020:1024	a pre-trained protein language model	989:1024	a pre-trained protein language model (pLM)	989:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	1	63	theme	N-linked	129:136	arg1	mechanism					187:195	an important post-translational mechanism	155:195	an important post-translational mechanism in Homo sapiens	155:211	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	1	63	theme	N-linked	129:136	arg1	glycosylation					138:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation	121:150	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	4	64	theme	N-linked	575:582	arg1	sites					598:602	N-linked glycosylation sites	575:602	N-linked glycosylation sites confined to N-X-[S/T] sequons	575:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	5	65	theme	learning-based	886:899	arg1	approach					901:908	a deep learning-based approach	879:908	a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM)	879:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	66	from	model	1020:1024	arg1	embeddings					973:982	embeddings	973:982	embeddings from a pre-trained protein language model (pLM)	973:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	5	67	dep	approach	901:908	arg1	predict					913:919	predict	913:919	to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM)	910:1030	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	1	68	theme	many	241:244	arg1	processes					263:271	many vital biological processes	241:271	many vital biological processes	241:271	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	5	69	link	N-linked	921:928	arg1	sites					943:947	N-linked glycosylated sites	921:947	N-linked glycosylated sites	921:947	Here, we developed LMNglyPred, a deep learning-based approach to predict N-linked glycosylated sites in human proteins using embeddings from a pre-trained protein language model (pLM).
37067908	0	70	gly	glycosylation	41:53	arg2	sites					55:59	human N-linked glycosylation sites	26:59	human N-linked glycosylation sites	26:59	LMNglyPred: prediction of human N-linked glycosylation sites using embeddings from a pre-trained protein language model.
37067908	2	71	theme	amino	352:356	arg1	acid					358:361	any amino acid	348:361	any amino acid	348:361	It occurs at the N-X-[S/T] sequon in amino acid sequences, where X can be any amino acid except proline.
37067908	1	72	theme	vital	246:250	arg1	processes					263:271	many vital biological processes	241:271	many vital biological processes	241:271	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	7	73	link	N-linked	1344:1351	arg1	sites					1367:1371	N-linked glycosylation sites	1344:1371	N-linked glycosylation sites confined to the N-X-[S/T] sequon	1344:1404	These results demonstrate that LMNglyPred is a robust computational tool to predict N-linked glycosylation sites confined to the N-X-[S/T] sequon.
37067908	4	74	theme	glycosylation	584:596	arg1	sites					598:602	N-linked glycosylation sites	575:602	N-linked glycosylation sites confined to N-X-[S/T] sequons	575:632	In that regard, computational prediction of N-linked glycosylation sites confined to N-X-[S/T] sequons is an important problem which has not been extensively addressed by the existing methods especially in regard to the creation of negative sets and leveraging the distilled information from protein language models.
37067908	1	75	from	mechanism	187:195	arg1	sapiens					205:211	Homo sapiens	200:211	Homo sapiens	200:211	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37067908	6	76	theme	Matthews	1079:1086	arg1	Coefficient					1100:1110	Matthews Correlation Coefficient	1079:1110	Matthews Correlation Coefficient	1079:1110	LMNglyPred produces sensitivity, specificity, Matthews Correlation Coefficient, precision, and accuracy of 76.50 percent, 75.36 percent, 0.49, 60.99 percent, and 75.74 percent, respectively on a benchmark independent test set.
37067908	1	77	theme	biological	252:261	arg1	processes					263:271	many vital biological processes	241:271	many vital biological processes	241:271	Protein N-linked glycosylation is an important post-translational mechanism in Homo sapiens, playing essential roles in many vital biological processes.
37230359	2	0	theme	NaCl	546:549	arg1	100 mM					569:574	100 mM	569:574	100 mM	569:574	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	0	theme	NaCl	546:549	arg1	10					562:563	10	562:563	10	562:563	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	0	theme	NaCl	546:549	arg1	solutions					551:559	NaCl solutions	546:559	NaCl solutions (10 and 100 mM)	546:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	3	1	theme	moisture	615:622	arg1	content					624:630	moisture content	615:630	moisture content	615:630	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	0	2	theme	extracellular	179:191	arg1	substances					203:212	extracellular polymeric substances	179:212	extracellular polymeric substances	179:212	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	2	3	theme	transport/retention	373:391	arg1	behaviors					393:401	The transport/retention behaviors	369:401	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM)	369:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	1	4	theme	mechanisms	261:270	arg1	effects					219:225	The effects	215:225	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents	215:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	3	5	theme	FT	656:657	arg1	treatment					659:667	FT treatment	656:667	FT treatment	656:667	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	0	6	theme	substances	203:212	arg1	roles					157:161	the roles	153:161	the roles of flagella and extracellular polymeric substances	153:212	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	1	7	theme	bacteria	275:282	arg1	transport/retention					284:302	bacteria transport/retention	275:302	bacteria transport/retention in porous media with different moisture contents	275:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	0	8	theme	Freeze-thaw	0:10	arg1	treatment					12:20	Freeze-thaw treatment	0:20	Freeze-thaw treatment of bacteria	0:32	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	4	9	dep	components	1085:1094	arg1	polysaccharides					1110:1124	polysaccharides	1110:1124	polysaccharides	1110:1124	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	9	dep	components	1085:1094	arg1	proteins					1097:1104	proteins	1097:1104	proteins	1097:1104	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	1	10	with	media	314:318	arg1	contents					344:351	different moisture contents	325:351	different moisture contents	325:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	4	11	theme	contribution	839:850	arg1	investigation					818:830	deep investigation	813:830	deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides)	813:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	7	12	theme	FT	1590:1591	arg1	treatment					1593:1601	FT treatment	1590:1601	FT treatment	1590:1601	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	4	13	theme	flagella	855:862	arg1	contribution					839:850	the contribution	835:850	the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides)	835:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	1	14	theme	freeze-thaw	230:240	arg1	treatment					247:255	freeze-thaw (FT) treatment	230:255	freeze-thaw (FT) treatment	230:255	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	4	15	theme	deep	813:816	arg1	investigation					818:830	deep investigation	813:830	deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides)	813:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	0	16	from	behaviors	76:84	arg1	media					127:131	both saturated and unsaturated porous media	89:131	both saturated and unsaturated porous media	89:131	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	7	17	from	deposition	1628:1637	arg1	columns					1647:1653	sand columns	1642:1653	sand columns with different moisture contents	1642:1686	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	3	18	theme	sand	702:705	arg1	columns					707:713	sand columns	702:713	sand columns	702:713	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	3	19	theme	chamber	785:791	arg1	systems					800:806	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	7	20	theme	copresent	1568:1576	arg1	acid					1584:1587	copresent humic acid	1568:1587	copresent humic acid	1568:1587	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	7	21	with	columns	1647:1653	arg1	contents					1679:1686	different moisture contents	1660:1686	different moisture contents	1660:1686	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	1	22	from	effects	219:225	arg1	transport/retention					284:302	bacteria transport/retention	275:302	bacteria transport/retention in porous media with different moisture contents	275:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	6	23	theme	enhanced	1527:1534	arg1	deposition					1546:1555	the enhanced bacterial deposition	1523:1555	the enhanced bacterial deposition	1523:1555	Instead, FT treatment stimulated EPS secretion and increased its hydrophobicity (via increasing hydrophobicity of both proteins and polysaccharides), mainly contributing to the enhanced bacterial deposition.
37230359	3	24	theme	plate	774:778	arg1	chamber					785:791	parallel plate flow chamber	765:791	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	3	24	theme	plate	774:778	arg1	PPFC					794:797	PPFC	794:797	PPFC	794:797	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	4	25	theme	major	1079:1083	arg1	components					1085:1094	two major components	1075:1094	its two major components (proteins and polysaccharides)	1071:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	5	26	theme	flagella	1247:1254	arg1	loss					1256:1259	flagella loss	1247:1259	flagella loss	1247:1259	Although FT treatment induced flagella loss, it was not the major contributor to driving to the enhanced FT-treated cell deposition.
37230359	2	27	with	columns	472:478	arg1	contents					504:511	different moisture contents	485:511	different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM)	485:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	3	28	theme	systems	800:806	arg1	results					736:742	the results	732:742	the results of QCM-D and visible parallel plate flow chamber (PPFC) systems	732:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	2	29	theme	different	485:493	arg1	contents					504:511	different moisture contents	485:511	different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM)	485:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	0	30	theme	flagella	166:173	arg1	roles					157:161	the roles	153:161	the roles of flagella and extracellular polymeric substances	153:212	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	3	31	theme	visible	757:763	arg1	systems					800:806	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	7	32	theme	moisture	1670:1677	arg1	contents					1679:1686	different moisture contents	1660:1686	different moisture contents	1660:1686	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	4	33	theme	treatment	1149:1157	arg1	mechanisms					1132:1141	the mechanisms	1128:1141	the mechanisms of FT treatment controlling bacterial transport/deposition	1128:1200	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	3	34	theme	QCM-D	747:751	arg1	systems					800:806	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	4	35	theme	bacteria	895:902	arg1	strain					904:909	genetic-modified bacteria strain	878:909	genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS)	878:979	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	36	theme	bacterial	1171:1179	arg1	transport/deposition					1181:1200	bacterial transport/deposition	1171:1200	bacterial transport/deposition	1171:1200	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	2	37	theme	treatment	433:441	arg1	cycles					443:448	different FT treatment cycles	420:448	different FT treatment cycles (0, 1, and 3)	420:462	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	0	38	theme	porous	120:125	arg1	media					127:131	both saturated and unsaturated porous media	89:131	both saturated and unsaturated porous media	89:131	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	7	39	theme	sand	1642:1645	arg1	columns					1647:1653	sand columns	1642:1653	sand columns with different moisture contents	1642:1686	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	3	40	from	deposition	688:697	arg1	columns					707:713	sand columns	702:713	sand columns	702:713	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	2	41	theme	different	420:428	arg1	cycles					443:448	different FT treatment cycles	420:448	different FT treatment cycles (0, 1, and 3)	420:462	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	6	42	theme	EPS	1383:1385	arg1	secretion					1387:1395	EPS secretion	1383:1395	EPS secretion	1383:1395	Instead, FT treatment stimulated EPS secretion and increased its hydrophobicity (via increasing hydrophobicity of both proteins and polysaccharides), mainly contributing to the enhanced bacterial deposition.
37230359	1	43	theme	porous	307:312	arg1	media					314:318	porous media	307:318	porous media with different moisture contents	307:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	4	44	theme	polymeric	954:962	arg1	EPS					976:978	EPS	976:978	EPS	976:978	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	44	theme	polymeric	954:962	arg1	substances					964:973	extracellular polymeric substances	940:973	extracellular polymeric substances (EPS)	940:979	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	5	45	theme	FT-treated	1322:1331	arg1	deposition					1338:1347	the enhanced FT-treated cell deposition	1309:1347	the enhanced FT-treated cell deposition	1309:1347	Although FT treatment induced flagella loss, it was not the major contributor to driving to the enhanced FT-treated cell deposition.
37230359	0	46	theme	bacterial	56:64	arg1	behaviors					76:84	bacterial transport behaviors	56:84	bacterial transport behaviors in both saturated and unsaturated porous media	56:131	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	1	47	theme	moisture	335:342	arg1	contents					344:351	different moisture contents	325:351	different moisture contents	325:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	2	48	with	bacteria	406:413	arg1	cycles					443:448	different FT treatment cycles	420:448	different FT treatment cycles (0, 1, and 3)	420:462	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	49	theme	bacteria	406:413	arg1	behaviors					393:401	The transport/retention behaviors	369:401	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM)	369:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	3	50	theme	solution	636:643	arg1	chemistry					645:653	solution chemistry	636:653	solution chemistry	636:653	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	0	51	theme	polymeric	193:201	arg1	substances					203:212	extracellular polymeric substances	179:212	extracellular polymeric substances	179:212	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	4	52	theme	overall	1003:1009	arg1	composition					1021:1031	composition	1021:1031	composition as well as the secondary structure of its two major components (proteins and polysaccharides)	1021:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	52	theme	overall	1003:1009	arg1	structure					1058:1066	the secondary structure	1044:1066	composition as well as the secondary structure of its two major components (proteins and polysaccharides)	1021:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	52	theme	overall	1003:1009	arg1	quantity					1011:1018	its overall quantity	999:1018	its overall quantity	999:1018	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	2	53	from	behaviors	393:401	arg1	columns					472:478	sand columns	467:478	sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM)	467:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	1	54	from	transport/retention	284:302	arg1	media					314:318	porous media	307:318	porous media with different moisture contents	307:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	6	55	theme	polysaccharides	1482:1496	arg1	hydrophobicity					1446:1459	hydrophobicity	1446:1459	hydrophobicity of both proteins and polysaccharides	1446:1496	Instead, FT treatment stimulated EPS secretion and increased its hydrophobicity (via increasing hydrophobicity of both proteins and polysaccharides), mainly contributing to the enhanced bacterial deposition.
37230359	6	56	theme	proteins	1469:1476	arg1	hydrophobicity					1446:1459	hydrophobicity	1446:1459	hydrophobicity of both proteins and polysaccharides	1446:1496	Instead, FT treatment stimulated EPS secretion and increased its hydrophobicity (via increasing hydrophobicity of both proteins and polysaccharides), mainly contributing to the enhanced bacterial deposition.
37230359	4	57	theme	secondary	1048:1056	arg1	quantity					1011:1018	its overall quantity	999:1018	its overall quantity	999:1018	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	57	theme	secondary	1048:1056	arg1	structure					1058:1066	the secondary structure	1044:1066	composition as well as the secondary structure of its two major components (proteins and polysaccharides)	1021:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	3	58	theme	bacteria	679:686	arg1	deposition					688:697	bacteria deposition	679:697	bacteria deposition in sand columns	679:713	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	7	59	theme	humic	1578:1582	arg1	acid					1584:1587	copresent humic acid	1568:1587	copresent humic acid	1568:1587	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	2	60	from	contents	504:511	arg1	100 mM					569:574	100 mM	569:574	100 mM	569:574	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	60	from	contents	504:511	arg1	10					562:563	10	562:563	10	562:563	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	60	from	contents	504:511	arg1	solutions					551:559	NaCl solutions	546:559	NaCl solutions (10 and 100 mM)	546:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	5	61	theme	FT	1226:1227	arg1	treatment					1229:1237	FT treatment	1226:1237	FT treatment	1226:1237	Although FT treatment induced flagella loss, it was not the major contributor to driving to the enhanced FT-treated cell deposition.
37230359	6	62	theme	bacterial	1536:1544	arg1	deposition					1546:1555	the enhanced bacterial deposition	1523:1555	the enhanced bacterial deposition	1523:1555	Instead, FT treatment stimulated EPS secretion and increased its hydrophobicity (via increasing hydrophobicity of both proteins and polysaccharides), mainly contributing to the enhanced bacterial deposition.
37230359	4	63	theme	components	1085:1094	arg1	composition					1021:1031	composition	1021:1031	composition as well as the secondary structure of its two major components (proteins and polysaccharides)	1021:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	63	theme	components	1085:1094	arg1	quantity					1011:1018	its overall quantity	999:1018	its overall quantity	999:1018	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	63	theme	components	1085:1094	arg1	structure					1058:1066	the secondary structure	1044:1066	composition as well as the secondary structure of its two major components (proteins and polysaccharides)	1021:1125	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	3	64	theme	flow	780:783	arg1	chamber					785:791	parallel plate flow chamber	765:791	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	3	64	theme	flow	780:783	arg1	PPFC					794:797	PPFC	794:797	PPFC	794:797	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	2	65	theme	moisture	495:502	arg1	contents					504:511	different moisture contents	485:511	different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM)	485:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	0	66	theme	saturated	94:102	arg1	media					127:131	both saturated and unsaturated porous media	89:131	both saturated and unsaturated porous media	89:131	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	3	67	theme	parallel	765:772	arg1	chamber					785:791	parallel plate flow chamber	765:791	QCM-D and visible parallel plate flow chamber (PPFC) systems	747:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	3	67	theme	parallel	765:772	arg1	PPFC					794:797	PPFC	794:797	PPFC	794:797	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	0	68	theme	unsaturated	108:118	arg1	media					127:131	both saturated and unsaturated porous media	89:131	both saturated and unsaturated porous media	89:131	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	3	69	with	consistent	716:725	arg1	results					736:742	the results	732:742	the results of QCM-D and visible parallel plate flow chamber (PPFC) systems	732:806	Regardless of moisture content and solution chemistry, FT treatment increased bacteria deposition in sand columns, consistent with the results of QCM-D and visible parallel plate flow chamber (PPFC) systems.
37230359	7	70	theme	different	1660:1668	arg1	contents					1679:1686	different moisture contents	1660:1686	different moisture contents	1660:1686	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	4	71	theme	genetic-modified	878:893	arg1	strain					904:909	genetic-modified bacteria strain	878:909	genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS)	878:979	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	5	72	theme	major	1277:1281	arg1	it					1262:1263	it	1262:1263	it	1262:1263	Although FT treatment induced flagella loss, it was not the major contributor to driving to the enhanced FT-treated cell deposition.
37230359	5	72	theme	major	1277:1281	arg1	contributor					1283:1293	the major contributor	1273:1293	the major contributor to driving to the enhanced FT-treated cell deposition	1273:1347	Although FT treatment induced flagella loss, it was not the major contributor to driving to the enhanced FT-treated cell deposition.
37230359	2	73	theme	FT	430:431	arg1	cycles					443:448	different FT treatment cycles	420:448	different FT treatment cycles (0, 1, and 3)	420:462	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	4	74	theme	FT	1146:1147	arg1	treatment					1149:1157	FT treatment	1146:1157	FT treatment	1146:1157	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	0	75	theme	bacteria	25:32	arg1	treatment					12:20	Freeze-thaw treatment	0:20	Freeze-thaw treatment of bacteria	0:32	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	6	76	theme	FT	1359:1360	arg1	treatment					1362:1370	FT treatment	1359:1370	FT treatment	1359:1370	Instead, FT treatment stimulated EPS secretion and increased its hydrophobicity (via increasing hydrophobicity of both proteins and polysaccharides), mainly contributing to the enhanced bacterial deposition.
37230359	7	77	theme	bacterial	1618:1626	arg1	deposition					1628:1637	bacterial deposition	1618:1637	bacterial deposition in sand columns with different moisture contents	1618:1686	Even with copresent humic acid, FT treatment still enhanced bacterial deposition in sand columns with different moisture contents.
37230359	2	78	theme	sand	467:470	arg1	columns					472:478	sand columns	467:478	sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM)	467:575	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	79	dep	cycles	443:448	arg1	1					454:454	1	454:454	1	454:454	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	79	dep	cycles	443:448	arg1	3					461:461	3	461:461	3	461:461	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	5	80	theme	enhanced	1313:1320	arg1	deposition					1338:1347	the enhanced FT-treated cell deposition	1309:1347	the enhanced FT-treated cell deposition	1309:1347	Although FT treatment induced flagella loss, it was not the major contributor to driving to the enhanced FT-treated cell deposition.
37230359	0	81	theme	transport	66:74	arg1	behaviors					76:84	bacterial transport behaviors	56:84	bacterial transport behaviors in both saturated and unsaturated porous media	56:131	Freeze-thaw treatment of bacteria significantly affects bacterial transport behaviors in both saturated and unsaturated porous media: Deep investigation the roles of flagella and extracellular polymeric substances.
37230359	1	82	theme	different	325:333	arg1	contents					344:351	different moisture contents	325:351	different moisture contents	325:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	4	83	theme	extracellular	940:952	arg1	EPS					976:978	EPS	976:978	EPS	976:978	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	4	83	theme	extracellular	940:952	arg1	substances					964:973	extracellular polymeric substances	940:973	extracellular polymeric substances (EPS)	940:979	Via deep investigation of the contribution of flagella through using genetic-modified bacteria strain without flagella and that of extracellular polymeric substances (EPS) through analyzing its overall quantity, composition as well as the secondary structure of its two major components (proteins and polysaccharides), the mechanisms of FT treatment controlling bacterial transport/deposition were revealed.
37230359	5	84	theme	cell	1333:1336	arg1	deposition					1338:1347	the enhanced FT-treated cell deposition	1309:1347	the enhanced FT-treated cell deposition	1309:1347	Although FT treatment induced flagella loss, it was not the major contributor to driving to the enhanced FT-treated cell deposition.
37230359	2	85	dep	contents	504:511	arg1	%					540:540	30 %	537:540	30 %	537:540	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	85	dep	contents	504:511	arg1	%					518:518	100 %	514:518	100 %	514:518	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	85	dep	contents	504:511	arg1	%					524:524	90 %	521:524	90 %	521:524	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	2	85	dep	contents	504:511	arg1	%					530:530	60 %	527:530	60 %	527:530	The transport/retention behaviors of bacteria with different FT treatment cycles (0, 1, and 3) in sand columns with different moisture contents (100 %, 90 %, 60 %, and 30 %) in NaCl solutions (10 and 100 mM) thus were investigated.
37230359	1	86	theme	FT	243:244	arg1	treatment					247:255	freeze-thaw (FT) treatment	230:255	freeze-thaw (FT) treatment	230:255	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
37230359	1	87	theme	treatment	247:255	arg1	effects					219:225	The effects	215:225	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents	215:351	The effects of freeze-thaw (FT) treatment and mechanisms on bacteria transport/retention in porous media with different moisture contents remain unclear.
36711817	0	0	theme	Postmenopausal	85:98	arg1	Women					100:104	Postmenopausal Women	85:104	Postmenopausal Women	85:104	Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
36711817	7	1	theme	urinary	935:941	arg1	GAGs					943:946	urinary GAGs	935:946	urinary GAGs	935:946	We also associated urinary GAGs with urobiome composition and identified bacterial species that significantly associated with urinary GAG concentration.
36711817	8	2	theme	Porphyromonas	1097:1109	arg1	somerae					1111:1117	Porphyromonas somerae	1097:1117	Porphyromonas somerae	1097:1117	Corynebacterium amycolatum, Porphyromonas somerae , and Staphylococcus pasteuri were positively associated with heparin sulfate or hyaluronic acid and bacterial species associated with vaginal dysbiosis were negatively correlated to urinary CS.
36711817	4	3	theme	GAG	565:567	arg1	composition					569:579	the urinary GAG composition	553:579	the urinary GAG composition in postmenopausal women	553:603	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	5	4	theme	postmenopausal	746:759	arg1	women					761:765	postmenopausal women	746:765	postmenopausal women	746:765	To investigate the relationship between GAGs and recurrent UTI (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women.
36711817	4	5	theme	urinary	507:513	arg1	composition					519:529	urinary GAG composition	507:529	urinary GAG composition in mixed cohorts	507:546	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	5	6	from	GAGs	715:718	arg1	cohort					736:741	a controlled cohort	723:741	a controlled cohort of postmenopausal women	723:765	To investigate the relationship between GAGs and recurrent UTI (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women.
36711817	4	7	theme	urinary	557:563	arg1	composition					569:579	the urinary GAG composition	553:579	the urinary GAG composition in postmenopausal women	553:603	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	5	8	theme	urinary	707:713	arg1	GAGs					715:718	urinary GAGs	707:718	urinary GAGs in a controlled cohort of postmenopausal women	707:765	To investigate the relationship between GAGs and recurrent UTI (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women.
36711817	6	9	theme	urinary	865:871	arg1	CS					873:874	urinary CS	865:874	urinary CS	865:874	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	4	10	from	composition	569:579	arg1	women					599:603	postmenopausal women	584:603	postmenopausal women	584:603	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	2	11	theme	epithelium	289:298	arg1	surface					266:272	The luminal surface	254:272	The luminal surface of the bladder epithelium	254:298	The luminal surface of the bladder epithelium is coated with a GAG layer.
36711817	6	12	theme	chondroitin	782:792	arg1	GAG					828:830	the major urinary GAG	810:830	the major urinary GAG in postmenopausal women	810:854	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	6	12	theme	chondroitin	782:792	arg1	CS					803:804	CS	803:804	CS	803:804	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	6	12	theme	chondroitin	782:792	arg1	sulfate					794:800	chondroitin sulfate	782:800	chondroitin sulfate (CS)	782:805	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	1	13	dep	linear	137:142	arg1	charged					156:162	charged	156:162	charged	156:162	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	8	14	theme	heparin	1181:1187	arg1	sulfate					1189:1195	heparin sulfate	1181:1195	heparin sulfate	1181:1195	Corynebacterium amycolatum, Porphyromonas somerae , and Staphylococcus pasteuri were positively associated with heparin sulfate or hyaluronic acid and bacterial species associated with vaginal dysbiosis were negatively correlated to urinary CS.
36711817	0	15	from	UTI	57:59	arg1	Women					100:104	Postmenopausal Women	85:104	Postmenopausal Women	85:104	Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
36711817	2	16	theme	bladder	281:287	arg1	epithelium					289:298	the bladder epithelium	277:298	the bladder epithelium	277:298	The luminal surface of the bladder epithelium is coated with a GAG layer.
36711817	6	17	with	women	892:896	arg1	rUTI					910:913	active rUTI	903:913	active rUTI	903:913	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	8	18	theme	vaginal	1254:1260	arg1	dysbiosis					1262:1270	vaginal dysbiosis	1254:1270	vaginal dysbiosis	1254:1270	Corynebacterium amycolatum, Porphyromonas somerae , and Staphylococcus pasteuri were positively associated with heparin sulfate or hyaluronic acid and bacterial species associated with vaginal dysbiosis were negatively correlated to urinary CS.
36711817	4	19	theme	Previous	476:483	arg1	studies					485:491	Previous studies	476:491	Previous studies	476:491	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	5	20	theme	women	761:765	arg1	cohort					736:741	a controlled cohort	723:741	a controlled cohort of postmenopausal women	723:765	To investigate the relationship between GAGs and recurrent UTI (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women.
36711817	6	21	theme	major	814:818	arg1	sulfate					794:800	chondroitin sulfate	782:800	chondroitin sulfate (CS)	782:805	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	6	21	theme	major	814:818	arg1	GAG					828:830	the major urinary GAG	810:830	the major urinary GAG in postmenopausal women	810:854	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	4	22	theme	postmenopausal	584:597	arg1	women					599:603	postmenopausal women	584:603	postmenopausal women	584:603	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	3	23	theme	protective	375:384	arg1	barrier					386:392	a protective barrier	373:392	a protective barrier	373:392	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	6	24	theme	postmenopausal	835:848	arg1	women					850:854	postmenopausal women	835:854	postmenopausal women	835:854	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	2	25	theme	GAG	317:319	arg1	layer					321:325	a GAG layer	315:325	a GAG layer	315:325	The luminal surface of the bladder epithelium is coated with a GAG layer.
36711817	3	26	theme	potential	409:417	arg1	site					431:434	a potential interaction site	407:434	a potential interaction site with the urinary microbiome (urobiome)	407:473	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	3	26	theme	potential	409:417	arg1	GAGs					345:348	These urothelial GAGs	328:348	These urothelial GAGs	328:348	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	0	27	theme	Urinary	0:6	arg1	Glycosaminoglycans					8:25	Urinary Glycosaminoglycans	0:25	Urinary Glycosaminoglycans	0:25	Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
36711817	3	28	theme	interaction	419:429	arg1	site					431:434	a potential interaction site	407:434	a potential interaction site with the urinary microbiome (urobiome)	407:473	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	3	28	theme	interaction	419:429	arg1	GAGs					345:348	These urothelial GAGs	328:348	These urothelial GAGs	328:348	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	9	29	theme	rUTI	1494:1497	arg1	pathobiology					1499:1510	rUTI pathobiology	1494:1510	rUTI pathobiology	1494:1510	Altogether, this work defines changes in urinary GAG composition associated with rUTI and identifies new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology.
36711817	7	30	theme	urinary	1042:1048	arg1	concentration					1054:1066	urinary GAG concentration	1042:1066	urinary GAG concentration	1042:1066	We also associated urinary GAGs with urobiome composition and identified bacterial species that significantly associated with urinary GAG concentration.
36711817	8	31	theme	bacterial	1220:1228	arg1	species					1230:1236	bacterial species	1220:1236	bacterial species associated with vaginal dysbiosis	1220:1270	Corynebacterium amycolatum, Porphyromonas somerae , and Staphylococcus pasteuri were positively associated with heparin sulfate or hyaluronic acid and bacterial species associated with vaginal dysbiosis were negatively correlated to urinary CS.
36711817	6	32	from	GAG	828:830	arg1	women					850:854	postmenopausal women	835:854	postmenopausal women	835:854	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	9	33	theme	GAG	1363:1365	arg1	composition					1367:1377	urinary GAG composition	1355:1377	urinary GAG composition	1355:1377	Altogether, this work defines changes in urinary GAG composition associated with rUTI and identifies new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology.
36711817	7	34	theme	GAG	1050:1052	arg1	concentration					1054:1066	urinary GAG concentration	1042:1066	urinary GAG concentration	1042:1066	We also associated urinary GAGs with urobiome composition and identified bacterial species that significantly associated with urinary GAG concentration.
36711817	0	35	theme	Recurrent	47:55	arg1	UTI					57:59	Recurrent UTI	47:59	Recurrent UTI	47:59	Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
36711817	8	36	theme	urinary	1302:1308	arg1	CS					1310:1311	urinary CS	1302:1311	urinary CS	1302:1311	Corynebacterium amycolatum, Porphyromonas somerae , and Staphylococcus pasteuri were positively associated with heparin sulfate or hyaluronic acid and bacterial species associated with vaginal dysbiosis were negatively correlated to urinary CS.
36711817	1	37	theme	disaccharide	202:213	arg1	units					215:219	disaccharide units	202:219	disaccharide units of uronic acid and amino sugars	202:251	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	1	38	theme	uronic	224:229	arg1	acid					231:234	uronic acid	224:234	uronic acid	224:234	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	9	39	from	changes	1344:1350	arg1	composition					1367:1377	urinary GAG composition	1355:1377	urinary GAG composition	1355:1377	Altogether, this work defines changes in urinary GAG composition associated with rUTI and identifies new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology.
36711817	6	40	theme	active	903:908	arg1	rUTI					910:913	active rUTI	903:913	active rUTI	903:913	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	0	41	from	Ecology	74:80	arg1	Women					100:104	Postmenopausal Women	85:104	Postmenopausal Women	85:104	Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
36711817	9	42	theme	urinary	1355:1361	arg1	composition					1367:1377	urinary GAG composition	1355:1377	urinary GAG composition	1355:1377	Altogether, this work defines changes in urinary GAG composition associated with rUTI and identifies new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology.
36711817	9	43	theme	urinary	1440:1446	arg1	GAGs					1448:1451	urinary GAGs	1440:1451	urinary GAGs	1440:1451	Altogether, this work defines changes in urinary GAG composition associated with rUTI and identifies new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology.
36711817	1	44	theme	acid	231:234	arg1	units					215:219	disaccharide units	202:219	disaccharide units of uronic acid and amino sugars	202:251	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	4	45	from	composition	519:529	arg1	cohorts					540:546	mixed cohorts	534:546	mixed cohorts	534:546	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	4	46	theme	GAG	515:517	arg1	composition					519:529	urinary GAG composition	507:529	urinary GAG composition in mixed cohorts	507:546	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	5	47	theme	recurrent	673:681	arg1	rUTI					688:691	rUTI	688:691	rUTI	688:691	To investigate the relationship between GAGs and recurrent UTI (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women.
36711817	5	47	theme	recurrent	673:681	arg1	UTI					683:685	recurrent UTI	673:685	recurrent UTI (rUTI)	673:692	To investigate the relationship between GAGs and recurrent UTI (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women.
36711817	0	48	theme	Urobiome	65:72	arg1	Ecology					74:80	Urobiome Ecology	65:80	Urobiome Ecology	65:80	Urinary Glycosaminoglycans are Associated with Recurrent UTI and Urobiome Ecology in Postmenopausal Women.
36711817	7	49	theme	bacterial	989:997	arg1	species					999:1005	bacterial species	989:1005	bacterial species that significantly associated with urinary GAG concentration	989:1066	We also associated urinary GAGs with urobiome composition and identified bacterial species that significantly associated with urinary GAG concentration.
36711817	4	50	theme	mixed	534:538	arg1	cohorts					540:546	mixed cohorts	534:546	mixed cohorts	534:546	Previous studies have profiled urinary GAG composition in mixed cohorts, but the urinary GAG composition in postmenopausal women remains undefined.
36711817	9	51	theme	new	1415:1417	arg1	associations					1419:1430	new associations	1415:1430	new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology	1415:1510	Altogether, this work defines changes in urinary GAG composition associated with rUTI and identifies new associations between urinary GAGs and the urobiome that may play a role in rUTI pathobiology.
36711817	7	52	theme	urobiome	953:960	arg1	composition					962:972	urobiome composition	953:972	urobiome composition	953:972	We also associated urinary GAGs with urobiome composition and identified bacterial species that significantly associated with urinary GAG concentration.
36711817	6	53	theme	urinary	820:826	arg1	sulfate					794:800	chondroitin sulfate	782:800	chondroitin sulfate (CS)	782:805	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	6	53	theme	urinary	820:826	arg1	GAG					828:830	the major urinary GAG	810:830	the major urinary GAG in postmenopausal women	810:854	We found that chondroitin sulfate (CS) is the major urinary GAG in postmenopausal women and that urinary CS was elevated in women with active rUTI.
36711817	3	54	with	site	431:434	arg1	urobiome					465:472	urobiome	465:472	urobiome	465:472	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	3	54	with	site	431:434	arg1	microbiome					453:462	the urinary microbiome	441:462	the urinary microbiome (urobiome)	441:473	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	1	55	theme	linear	137:142	arg1	Glycosaminoglycans					107:124	Glycosaminoglycans	107:124	Glycosaminoglycans (GAGs)	107:131	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	1	55	theme	linear	137:142	arg1	polysaccharides					164:178	linear, negatively charged polysaccharides	137:178	linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars	137:251	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	1	56	theme	amino	240:244	arg1	sugars					246:251	amino sugars	240:251	amino sugars	240:251	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	5	57	theme	controlled	725:734	arg1	cohort					736:741	a controlled cohort	723:741	a controlled cohort of postmenopausal women	723:765	To investigate the relationship between GAGs and recurrent UTI (rUTI), we profiled urinary GAGs in a controlled cohort of postmenopausal women.
36711817	3	58	theme	urinary	445:451	arg1	urobiome					465:472	urobiome	465:472	urobiome	465:472	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	3	58	theme	urinary	445:451	arg1	microbiome					453:462	the urinary microbiome	441:462	the urinary microbiome (urobiome)	441:473	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	2	59	theme	luminal	258:264	arg1	surface					266:272	The luminal surface	254:272	The luminal surface of the bladder epithelium	254:298	The luminal surface of the bladder epithelium is coated with a GAG layer.
36711817	1	60	theme	sugars	246:251	arg1	units					215:219	disaccharide units	202:219	disaccharide units of uronic acid and amino sugars	202:251	Glycosaminoglycans (GAGs) are linear, negatively charged polysaccharides composed of repeating disaccharide units of uronic acid and amino sugars.
36711817	3	61	theme	urothelial	334:343	arg1	site					431:434	a potential interaction site	407:434	a potential interaction site with the urinary microbiome (urobiome)	407:473	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	3	61	theme	urothelial	334:343	arg1	GAGs					345:348	These urothelial GAGs	328:348	These urothelial GAGs	328:348	These urothelial GAGs are thought to provide a protective barrier and serve as a potential interaction site with the urinary microbiome (urobiome).
36711817	8	62	theme	hyaluronic	1200:1209	arg1	acid					1211:1214	hyaluronic acid	1200:1214	hyaluronic acid	1200:1214	Corynebacterium amycolatum, Porphyromonas somerae , and Staphylococcus pasteuri were positively associated with heparin sulfate or hyaluronic acid and bacterial species associated with vaginal dysbiosis were negatively correlated to urinary CS.
36893425	12	0	theme	cancer	1635:1640	arg1	cells					1642:1646	colorectal cancer cells	1624:1646	colorectal cancer cells	1624:1646	Furthermore, the nanoparticles significantly promoted the apoptosis of colorectal cancer cells.
36893425	13	1	theme	important	1775:1783	arg1	nutraceutical					1785:1797	an important nutraceutical	1772:1797	an important nutraceutical	1772:1797	This study suggests that food-grade biopolymer nanoparticles can be used to improve the bioavailability and bioactivity of an important nutraceutical.
36893425	0	2	theme	Curcumin	97:104	arg1	Delivery					106:113	Curcumin Delivery	97:113	Curcumin Delivery	97:113	Lactoferrin-Based Ternary Composite Nanoparticles with Enhanced Dispersibility and Stability for Curcumin Delivery.
36893425	1	3	theme	anti-inflammatory	180:196	arg1	activities					214:223	free radical antioxidant, anti-inflammatory, and anticancer activities	154:223	free radical antioxidant, anti-inflammatory, and anticancer activities	154:223	Curcumin has been reported to exhibit free radical antioxidant, anti-inflammatory, and anticancer activities, which are beneficial for nutraceutical applications.
36893425	4	4	theme	protective	615:624	arg1	polyphenols					674:684	polyphenols	674:684	polyphenols	674:684	These colloidal particles can be assembled from structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols.
36893425	4	4	theme	protective	615:624	arg1	proteins					643:650	proteins	643:650	proteins	643:650	These colloidal particles can be assembled from structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols.
36893425	4	4	theme	protective	615:624	arg1	effects					626:632	protective effects	615:632	protective effects	615:632	These colloidal particles can be assembled from structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols.
36893425	4	4	theme	protective	615:624	arg1	polysaccharides					653:667	polysaccharides	653:667	polysaccharides	653:667	These colloidal particles can be assembled from structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols.
36893425	5	5	theme	-epigallocatechin	723:739	arg1	EGCG					750:753	EGCG	750:753	EGCG	750:753	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	5	5	theme	-epigallocatechin	723:739	arg1	gallate					741:747	(-)-epigallocatechin gallate	720:747	(-)-epigallocatechin gallate (EGCG)	720:754	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	10	6	theme	nanoparticles	1365:1377	arg1	uptake					1311:1316	cellular uptake	1302:1316	cellular uptake	1302:1316	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	10	6	theme	nanoparticles	1365:1377	arg1	effects					1334:1340	anticancer effects	1323:1340	anticancer effects	1323:1340	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	10	6	theme	nanoparticles	1365:1377	arg1	properties					1290:1299	The in vitro digestion properties	1267:1299	The in vitro digestion properties	1267:1299	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	11	7	theme	cellular	1451:1458	arg1	uptake					1460:1465	cellular uptake	1451:1465	cellular uptake	1451:1465	Compared to free curcumin, the bioaccessibility and cellular uptake of the curcumin were significantly improved after encapsulation in the nanoparticles.
36893425	10	8	theme	curcumin-loaded	1349:1363	arg1	nanoparticles					1365:1377	the curcumin-loaded nanoparticles	1345:1377	the curcumin-loaded nanoparticles	1345:1377	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	8	9	theme	light	1124:1128	arg1	stabilities					1143:1153	the thermal, light, and storage stabilities	1111:1153	the thermal, light, and storage stabilities of the curcumin	1111:1169	Encapsulation improved the thermal, light, and storage stabilities of the curcumin.
36893425	7	10	theme	curcumin	1030:1037	arg1	efficiency					982:991	The encapsulation efficiency	964:991	The encapsulation efficiency (86%)	964:997	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	10	theme	curcumin	1030:1037	arg1	%					996:996	86%	994:996	86%	994:996	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	10	theme	curcumin	1030:1037	arg1	high					1082:1085	high	1082:1085	high	1082:1085	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	10	theme	curcumin	1030:1037	arg1	capacity					1011:1018	loading capacity	1003:1018	loading capacity (5.8%)	1003:1025	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	10	theme	curcumin	1030:1037	arg1	%					1024:1024	5.8%	1021:1024	5.8%	1021:1024	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	5	11	theme	simple	837:842	arg1	method					853:858	a simple pH-shift method	835:858	a simple pH-shift method	835:858	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	4	12	theme	colloidal	517:525	arg1	particles					527:535	These colloidal particles	511:535	These colloidal particles	511:535	These colloidal particles can be assembled from structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols.
36893425	0	13	theme	Enhanced	55:62	arg1	Dispersibility					64:77	Dispersibility	64:77	Dispersibility	64:77	Lactoferrin-Based Ternary Composite Nanoparticles with Enhanced Dispersibility and Stability for Curcumin Delivery.
36893425	13	14	theme	nutraceutical	1785:1797	arg1	bioactivity					1757:1767	bioactivity	1757:1767	bioactivity	1757:1767	This study suggests that food-grade biopolymer nanoparticles can be used to improve the bioavailability and bioactivity of an important nutraceutical.
36893425	13	14	theme	nutraceutical	1785:1797	arg1	bioavailability					1737:1751	bioavailability	1737:1751	bioavailability	1737:1751	This study suggests that food-grade biopolymer nanoparticles can be used to improve the bioavailability and bioactivity of an important nutraceutical.
36893425	5	15	theme	pH-shift	844:851	arg1	method					853:858	a simple pH-shift method	835:858	a simple pH-shift method	835:858	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	0	16	theme	Ternary	18:24	arg1	Composite					26:34	Lactoferrin-Based Ternary Composite	0:34	Lactoferrin-Based Ternary Composite	0:34	Lactoferrin-Based Ternary Composite Nanoparticles with Enhanced Dispersibility and Stability for Curcumin Delivery.
36893425	5	17	theme	hyaluronic	761:770	arg1	HA					778:779	HA	778:779	HA	778:779	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	5	17	theme	hyaluronic	761:770	arg1	acid					772:775	hyaluronic acid	761:775	hyaluronic acid (HA)	761:780	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	2	18	theme	poor	339:342	arg1	solubility					350:359	its poor water solubility	335:359	its poor water solubility	335:359	However, its application for this purpose is limited by its poor water solubility, stability, and bioavailability.
36893425	11	19	theme	free	1411:1414	arg1	curcumin					1416:1423	free curcumin	1411:1423	free curcumin	1411:1423	Compared to free curcumin, the bioaccessibility and cellular uptake of the curcumin were significantly improved after encapsulation in the nanoparticles.
36893425	1	20	theme	anticancer	203:212	arg1	activities					214:223	free radical antioxidant, anti-inflammatory, and anticancer activities	154:223	free radical antioxidant, anti-inflammatory, and anticancer activities	154:223	Curcumin has been reported to exhibit free radical antioxidant, anti-inflammatory, and anticancer activities, which are beneficial for nutraceutical applications.
36893425	0	21	theme	Lactoferrin-Based	0:16	arg1	Composite					26:34	Lactoferrin-Based Ternary Composite	0:34	Lactoferrin-Based Ternary Composite	0:34	Lactoferrin-Based Ternary Composite Nanoparticles with Enhanced Dispersibility and Stability for Curcumin Delivery.
36893425	13	22	used	used	1717:1720	arg2	nanoparticles					1696:1708	food-grade biopolymer nanoparticles	1674:1708	food-grade biopolymer nanoparticles	1674:1708	This study suggests that food-grade biopolymer nanoparticles can be used to improve the bioavailability and bioactivity of an important nutraceutical.
36893425	6	23	theme	=	953:953	arg1	nm					959:960	d = 145 nm	951:960	d = 145 nm	951:960	We showed that curcumin could be successfully loaded into these LF-EGCG-HA nanoparticles (d = 145 nm).
36893425	6	23	theme	=	953:953	arg1	nanoparticles					936:948	these LF-EGCG-HA nanoparticles	919:948	these LF-EGCG-HA nanoparticles (d = 145 nm)	919:961	We showed that curcumin could be successfully loaded into these LF-EGCG-HA nanoparticles (d = 145 nm).
36893425	10	24	theme	digestion	1280:1288	arg1	properties					1290:1299	The in vitro digestion properties	1267:1299	The in vitro digestion properties	1267:1299	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	5	25	theme	composite	805:813	arg1	nanoparticles					815:827	composite nanoparticles	805:827	composite nanoparticles using a simple pH-shift method	805:858	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	8	26	theme	storage	1135:1141	arg1	stabilities					1143:1153	the thermal, light, and storage stabilities	1111:1153	the thermal, light, and storage stabilities of the curcumin	1111:1169	Encapsulation improved the thermal, light, and storage stabilities of the curcumin.
36893425	6	27	theme	d	951:951	arg1	nm					959:960	d = 145 nm	951:960	d = 145 nm	951:960	We showed that curcumin could be successfully loaded into these LF-EGCG-HA nanoparticles (d = 145 nm).
36893425	6	27	theme	d	951:951	arg1	nanoparticles					936:948	these LF-EGCG-HA nanoparticles	919:948	these LF-EGCG-HA nanoparticles (d = 145 nm)	919:961	We showed that curcumin could be successfully loaded into these LF-EGCG-HA nanoparticles (d = 145 nm).
36893425	10	28	theme	cellular	1302:1309	arg1	uptake					1311:1316	cellular uptake	1302:1316	cellular uptake	1302:1316	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	3	29	theme	food-grade	431:440	arg1	particles					452:460	food-grade colloidal particles	431:460	food-grade colloidal particles that encapsulate, protect, and deliver curcumin	431:508	These problems can be overcome using food-grade colloidal particles that encapsulate, protect, and deliver curcumin.
36893425	12	30	theme	colorectal	1624:1633	arg1	cells					1642:1646	colorectal cancer cells	1624:1646	colorectal cancer cells	1624:1646	Furthermore, the nanoparticles significantly promoted the apoptosis of colorectal cancer cells.
36893425	13	31	theme	food-grade	1674:1683	arg1	nanoparticles					1696:1708	food-grade biopolymer nanoparticles	1674:1708	food-grade biopolymer nanoparticles	1674:1708	This study suggests that food-grade biopolymer nanoparticles can be used to improve the bioavailability and bioactivity of an important nutraceutical.
36893425	10	32	theme	in	1271:1272	arg1	properties					1290:1299	The in vitro digestion properties	1267:1299	The in vitro digestion properties	1267:1299	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	3	33	theme	colloidal	442:450	arg1	particles					452:460	food-grade colloidal particles	431:460	food-grade colloidal particles that encapsulate, protect, and deliver curcumin	431:508	These problems can be overcome using food-grade colloidal particles that encapsulate, protect, and deliver curcumin.
36893425	4	34	theme	structure-forming	559:575	arg1	components					582:591	structure-forming food components	559:591	structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols	559:684	These colloidal particles can be assembled from structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols.
36893425	13	35	theme	biopolymer	1685:1694	arg1	nanoparticles					1696:1708	food-grade biopolymer nanoparticles	1674:1708	food-grade biopolymer nanoparticles	1674:1708	This study suggests that food-grade biopolymer nanoparticles can be used to improve the bioavailability and bioactivity of an important nutraceutical.
36893425	6	36	theme	LF-EGCG-HA	925:934	arg1	nm					959:960	d = 145 nm	951:960	d = 145 nm	951:960	We showed that curcumin could be successfully loaded into these LF-EGCG-HA nanoparticles (d = 145 nm).
36893425	6	36	theme	LF-EGCG-HA	925:934	arg1	nanoparticles					936:948	these LF-EGCG-HA nanoparticles	919:948	these LF-EGCG-HA nanoparticles (d = 145 nm)	919:961	We showed that curcumin could be successfully loaded into these LF-EGCG-HA nanoparticles (d = 145 nm).
36893425	9	37	theme	curcumin-loaded	1186:1200	arg1	nanoparticles					1202:1214	the curcumin-loaded nanoparticles	1182:1214	the curcumin-loaded nanoparticles	1182:1214	Moreover, the curcumin-loaded nanoparticles exhibited good redispersibility after dehydration.
36893425	10	38	dep	in	1271:1272	arg1	vitro					1274:1278	vitro	1274:1278	vitro	1274:1278	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	8	39	theme	curcumin	1162:1169	arg1	stabilities					1143:1153	the thermal, light, and storage stabilities	1111:1153	the thermal, light, and storage stabilities of the curcumin	1111:1169	Encapsulation improved the thermal, light, and storage stabilities of the curcumin.
36893425	7	40	theme	loading	1003:1009	arg1	capacity					1011:1018	loading capacity	1003:1018	loading capacity (5.8%)	1003:1025	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	40	theme	loading	1003:1009	arg1	%					1024:1024	5.8%	1021:1024	5.8%	1021:1024	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	41	theme	encapsulation	968:980	arg1	efficiency					982:991	The encapsulation efficiency	964:991	The encapsulation efficiency (86%)	964:997	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	41	theme	encapsulation	968:980	arg1	%					996:996	86%	994:996	86%	994:996	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	7	41	theme	encapsulation	968:980	arg1	high					1082:1085	high	1082:1085	high	1082:1085	The encapsulation efficiency (86%) and loading capacity (5.8%) of curcumin within these nanoparticles were relatively high.
36893425	4	42	theme	food	577:580	arg1	components					582:591	structure-forming food components	559:591	structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols	559:684	These colloidal particles can be assembled from structure-forming food components that may also exhibit protective effects, such as proteins, polysaccharides, and polyphenols.
36893425	5	43	used	used	787:790	arg2	lactoferrin					702:712	lactoferrin	702:712	lactoferrin (LF)	702:717	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	5	43	used	used	787:790	arg2	gallate					741:747	(-)-epigallocatechin gallate	720:747	(-)-epigallocatechin gallate (EGCG)	720:754	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	5	43	used	used	787:790	arg2	LF					715:716	LF	715:716	LF	715:716	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	5	43	used	used	787:790	arg2	EGCG					750:753	EGCG	750:753	EGCG	750:753	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	5	43	used	used	787:790	arg2	acid					772:775	hyaluronic acid	761:775	hyaluronic acid (HA)	761:780	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	5	43	used	used	787:790	arg2	HA					778:779	HA	778:779	HA	778:779	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	12	44	theme	cells	1642:1646	arg1	apoptosis					1611:1619	the apoptosis	1607:1619	the apoptosis of colorectal cancer cells	1607:1646	Furthermore, the nanoparticles significantly promoted the apoptosis of colorectal cancer cells.
36893425	11	45	theme	curcumin	1474:1481	arg1	bioaccessibility					1430:1445	bioaccessibility	1430:1445	bioaccessibility	1430:1445	Compared to free curcumin, the bioaccessibility and cellular uptake of the curcumin were significantly improved after encapsulation in the nanoparticles.
36893425	11	45	theme	curcumin	1474:1481	arg1	uptake					1460:1465	cellular uptake	1451:1465	cellular uptake	1451:1465	Compared to free curcumin, the bioaccessibility and cellular uptake of the curcumin were significantly improved after encapsulation in the nanoparticles.
36893425	8	46	theme	thermal	1115:1121	arg1	stabilities					1143:1153	the thermal, light, and storage stabilities	1111:1153	the thermal, light, and storage stabilities of the curcumin	1111:1169	Encapsulation improved the thermal, light, and storage stabilities of the curcumin.
36893425	5	47	dep	gallate	741:747	arg1	-					721:721	-	721:721	-	721:721	In this study, lactoferrin (LF), (-)-epigallocatechin gallate (EGCG), and hyaluronic acid (HA) were used to fabricate composite nanoparticles using a simple pH-shift method.
36893425	9	48	theme	good	1226:1229	arg1	redispersibility					1231:1246	good redispersibility	1226:1246	good redispersibility	1226:1246	Moreover, the curcumin-loaded nanoparticles exhibited good redispersibility after dehydration.
36893425	2	49	theme	water	344:348	arg1	solubility					350:359	its poor water solubility	335:359	its poor water solubility	335:359	However, its application for this purpose is limited by its poor water solubility, stability, and bioavailability.
36893425	1	50	theme	nutraceutical	251:263	arg1	applications					265:276	nutraceutical applications	251:276	nutraceutical applications	251:276	Curcumin has been reported to exhibit free radical antioxidant, anti-inflammatory, and anticancer activities, which are beneficial for nutraceutical applications.
36893425	11	51	dep	bioaccessibility	1430:1445	arg1	the					1426:1428	the	1426:1428	the	1426:1428	Compared to free curcumin, the bioaccessibility and cellular uptake of the curcumin were significantly improved after encapsulation in the nanoparticles.
36893425	10	52	theme	anticancer	1323:1332	arg1	effects					1334:1340	anticancer effects	1323:1340	anticancer effects	1323:1340	The in vitro digestion properties, cellular uptake, and anticancer effects of the curcumin-loaded nanoparticles were then explored.
36893425	1	53	theme	antioxidant	167:177	arg1	activities					214:223	free radical antioxidant, anti-inflammatory, and anticancer activities	154:223	free radical antioxidant, anti-inflammatory, and anticancer activities	154:223	Curcumin has been reported to exhibit free radical antioxidant, anti-inflammatory, and anticancer activities, which are beneficial for nutraceutical applications.
37144381	8	0	theme	increased	1278:1286	arg1	charge					1296:1301	increased anionic charge	1278:1301	increased anionic charge	1278:1301	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	2	1	theme	delivery	398:405	arg1	research					407:414	current drug delivery research	385:414	current drug delivery research	385:414	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	2	2	from	interest	373:380	arg1	research					407:414	current drug delivery research	385:414	current drug delivery research	385:414	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	10	3	theme	cell	1564:1567	arg1	proliferation					1569:1581	cell proliferation	1564:1581	cell proliferation	1564:1581	Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
37144381	9	4	theme	heparin	1333:1339	arg1	component					1341:1349	The heparin component	1329:1349	The heparin component	1329:1349	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	4	5	with	association	616:626	arg1	liposomes					678:686	unilamellar liposomes	666:686	unilamellar liposomes	666:686	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	1	6	theme	therapeutic	210:220	arg1	proteins					222:229	full-length therapeutic proteins	198:229	full-length therapeutic proteins	198:229	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	11	7	theme	GHK-mediated	1760:1771	arg1	delivery					1783:1790	GHK-mediated liposomal delivery	1760:1790	GHK-mediated liposomal delivery	1760:1790	Together, the results support that cell-surface glycosaminoglycans can be involved in GHK-mediated liposomal delivery, which can be further greatly enhanced by association with heparin.
37144381	1	8	with	peptides	104:111	arg1	structure					135:143	defined chemical structure	118:143	defined chemical structure	118:143	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	1	8	with	peptides	104:111	arg1	responses					158:166	cellular responses	149:166	cellular responses	149:166	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	6	9	theme	liposomes	1081:1089	arg1	bio-interactions					1032:1047	bio-interactions	1032:1047	bio-interactions of synthetic peptide-presenting liposomes	1032:1089	The results provide a useful tool for screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique.
37144381	12	10	theme	therapeutic	1969:1979	arg1	applications					1999:2010	therapeutic and cosmeceutical applications	1969:2010	therapeutic and cosmeceutical applications	1969:2010	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	2	11	theme	targets	346:352	arg1	determination					323:335	determination	323:335	determination of their targets	323:352	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	2	11	theme	targets	346:352	arg1	factors					311:317	other bioactive factors	295:317	other bioactive factors	295:317	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	5	12	theme	ITC	930:932	arg1	analysis					934:941	ITC analysis	930:941	ITC analysis of such interactions	930:962	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	6	13	theme	synthetic	1052:1060	arg1	liposomes					1081:1089	synthetic peptide-presenting liposomes	1052:1089	synthetic peptide-presenting liposomes	1052:1089	The results provide a useful tool for screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique.
37144381	4	14	theme	defined	741:747	arg1	properties					749:758	defined properties	741:758	defined properties	741:758	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	5	15	theme	liposomal	804:812	arg1	surface					814:820	the liposomal surface	800:820	the liposomal surface	800:820	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	6	16	theme	bio-interactions	1032:1047	arg1	screening					1019:1027	screening	1019:1027	screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique	1019:1110	The results provide a useful tool for screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique.
37144381	7	17	theme	multi-functional	1150:1165	arg1	GHK-heparin					1177:1187	a multi-functional nanosized GHK-heparin	1148:1187	a multi-functional nanosized GHK-heparin covering for liposomes	1148:1210	They were also employed to produce a multi-functional nanosized GHK-heparin covering for liposomes.
37144381	9	18	theme	liposomes	1407:1415	arg1	accumulation					1378:1389	the accumulation	1374:1389	the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity	1374:1514	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	12	19	theme	cosmeceutical	1985:1997	arg1	applications					1999:2010	therapeutic and cosmeceutical applications	1969:2010	therapeutic and cosmeceutical applications	1969:2010	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	5	20	theme	GHK	783:785	arg1	component					787:795	the GHK component	779:795	the GHK component on the liposomal surface	779:820	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	9	21	theme	3T3	1420:1422	arg1	fibroblasts					1424:1434	3T3 fibroblasts	1420:1434	3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity	1420:1514	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	2	22	theme	bioactive	301:309	arg1	factors					311:317	other bioactive factors	295:317	other bioactive factors	295:317	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	12	23	theme	GHK-based	1943:1951	arg1	liposomes					1874:1882	The composite liposomes	1860:1882	The composite liposomes with GHK-heparin covering	1860:1908	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	12	23	theme	GHK-based	1943:1951	arg1	formulation					1953:1963	an advanced GHK-based formulation	1931:1963	an advanced GHK-based formulation for therapeutic and cosmeceutical applications	1931:2010	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	1	24	theme	promising	172:180	arg1	alternatives					182:193	promising alternatives	172:193	promising alternatives to full-length therapeutic proteins	172:229	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	1	24	theme	promising	172:180	arg1	peptides					104:111	Small biospecific peptides	86:111	Small biospecific peptides with defined chemical structure and cellular responses	86:166	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	0	25	from	Evaluation	0:9	arg1	liposomal					66:74	liposomal	66:74	liposomal	66:74	Evaluation of GHK peptide-heparin interactions in multifunctional liposomal covering.
37144381	10	26	theme	GSH	1625:1627	arg1	depletion					1629:1637	GSH depletion	1625:1637	GSH depletion	1625:1637	Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
37144381	1	27	theme	biospecific	92:102	arg1	alternatives					182:193	promising alternatives	172:193	promising alternatives to full-length therapeutic proteins	172:229	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	1	27	theme	biospecific	92:102	arg1	peptides					104:111	Small biospecific peptides	86:111	Small biospecific peptides with defined chemical structure and cellular responses	86:166	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	10	28	theme	stress	1655:1660	arg1	conditions					1662:1671	oxidative stress conditions	1645:1671	oxidative stress conditions	1645:1671	Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
37144381	3	29	theme	liposomal	463:471	arg1	formulations					473:484	new liposomal formulations	459:484	new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	459:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	5	30	theme	RGD	905:907	arg1	counterpart					909:919	RGD counterpart	905:919	RGD counterpart	905:919	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	9	31	theme	cell-penetrating	1490:1505	arg1	activity					1507:1514	the highest cell-penetrating activity	1478:1514	the highest cell-penetrating activity	1478:1514	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	0	32	theme	GHK	14:16	arg1	interactions					34:45	GHK peptide-heparin interactions	14:45	GHK peptide-heparin interactions	14:45	Evaluation of GHK peptide-heparin interactions in multifunctional liposomal covering.
37144381	1	33	theme	chemical	126:133	arg1	structure					135:143	defined chemical structure	118:143	defined chemical structure	118:143	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	4	34	theme	membranotropic	631:644	arg1	derivative					650:659	membranotropic GHK derivative	631:659	membranotropic GHK derivative	631:659	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	3	35	theme	GHK	501:503	arg1	peptide					505:511	ECM-derived GHK peptide	489:511	ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	489:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	5	36	theme	such	946:949	arg1	interactions					951:962	such interactions	946:962	such interactions	946:962	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	0	37	theme	interactions	34:45	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of GHK peptide-heparin interactions in multifunctional liposomal covering.	0:84	Evaluation of GHK peptide-heparin interactions in multifunctional liposomal covering.
37144381	2	38	theme	drug	393:396	arg1	research					407:414	current drug delivery research	385:414	current drug delivery research	385:414	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	3	39	theme	regeneration-related	536:555	arg1	activities					557:566	its multiple regeneration-related activities	523:566	its multiple regeneration-related activities	523:566	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	8	40	theme	composite	1227:1235	arg1	liposomes					1237:1245	The resulting composite liposomes	1213:1245	The resulting composite liposomes	1213:1245	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	4	41	theme	In	608:609	arg1	association					616:626	In situ association	608:626	In situ association of membranotropic GHK derivative with unilamellar liposomes	608:686	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	10	42	theme	latter	1534:1539	arg1	formulation					1541:1551	the latter formulation	1530:1551	the latter formulation	1530:1551	Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
37144381	3	43	theme	recognized	579:588	arg1	targets					599:605	recognized cellular targets	579:605	ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	489:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	5	44	theme	interactions	951:962	arg1	analysis					934:941	ITC analysis	930:941	ITC analysis of such interactions	930:962	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	8	45	theme	size	1261:1264	arg1	dispersity					1266:1275	low size dispersity	1257:1275	low size dispersity	1257:1275	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	11	46	with	association	1834:1844	arg1	heparin					1851:1857	heparin	1851:1857	heparin	1851:1857	Together, the results support that cell-surface glycosaminoglycans can be involved in GHK-mediated liposomal delivery, which can be further greatly enhanced by association with heparin.
37144381	2	47	with	combination	278:288	arg1	determination					323:335	determination	323:335	determination of their targets	323:352	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	2	47	with	combination	278:288	arg1	factors					311:317	other bioactive factors	295:317	other bioactive factors	295:317	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	6	48	theme	useful	1003:1008	arg1	tool					1010:1013	a useful tool	1001:1013	a useful tool for screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique	1001:1110	The results provide a useful tool for screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique.
37144381	4	49	theme	derivative	650:659	arg1	association					616:626	In situ association	608:626	In situ association of membranotropic GHK derivative with unilamellar liposomes	608:686	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	5	50	from	component	787:795	arg1	surface					814:820	the liposomal surface	800:820	the liposomal surface	800:820	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	8	51	theme	anionic	1288:1294	arg1	charge					1296:1301	increased anionic charge	1278:1301	increased anionic charge	1278:1301	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	4	52	theme	unilamellar	666:676	arg1	liposomes					678:686	unilamellar liposomes	666:686	unilamellar liposomes	666:686	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	8	53	contain	possessed	1247:1255	arg1	liposomes					1237:1245	The resulting composite liposomes	1213:1245	The resulting composite liposomes	1213:1245	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	8	53	contain	possessed	1247:1255	arg2	charge					1296:1301	increased anionic charge	1278:1301	increased anionic charge	1278:1301	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	8	53	contain	possessed	1247:1255	arg2	dispersity					1266:1275	low size dispersity	1257:1275	low size dispersity	1257:1275	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	8	53	contain	possessed	1247:1255	arg2	rigidity					1319:1326	mechanical rigidity	1308:1326	mechanical rigidity	1308:1326	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	3	54	theme	targets	599:605	arg1	formulations					473:484	new liposomal formulations	459:484	new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	459:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	1	55	theme	full-length	198:208	arg1	proteins					222:229	full-length therapeutic proteins	198:229	full-length therapeutic proteins	198:229	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	9	56	from	accumulation	1378:1389	arg1	fibroblasts					1424:1434	3T3 fibroblasts	1420:1434	3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity	1420:1514	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	10	57	theme	ROS	1606:1608	arg1	production					1610:1619	ROS production	1606:1619	ROS production	1606:1619	Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
37144381	2	58	theme	substantial	361:371	arg1	interest					373:380	substantial interest	361:380	substantial interest in current drug delivery research	361:414	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	8	59	theme	mechanical	1308:1317	arg1	rigidity					1319:1326	mechanical rigidity	1308:1326	mechanical rigidity	1308:1326	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	3	60	link	ECM-derived	489:499	arg1	peptide					505:511	ECM-derived GHK peptide	489:511	ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	489:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	11	61	theme	liposomal	1773:1781	arg1	delivery					1783:1790	GHK-mediated liposomal delivery	1760:1790	GHK-mediated liposomal delivery	1760:1790	Together, the results support that cell-surface glycosaminoglycans can be involved in GHK-mediated liposomal delivery, which can be further greatly enhanced by association with heparin.
37144381	6	62	theme	peptide-presenting	1062:1079	arg1	liposomes					1081:1089	synthetic peptide-presenting liposomes	1052:1089	synthetic peptide-presenting liposomes	1052:1089	The results provide a useful tool for screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique.
37144381	4	63	theme	GHK-modified	713:724	arg1	liposomes					726:734	GHK-modified liposomes	713:734	GHK-modified liposomes	713:734	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	7	64	theme	nanosized	1167:1175	arg1	GHK-heparin					1177:1187	a multi-functional nanosized GHK-heparin	1148:1187	a multi-functional nanosized GHK-heparin covering for liposomes	1148:1210	They were also employed to produce a multi-functional nanosized GHK-heparin covering for liposomes.
37144381	9	65	theme	GHK-modified	1394:1405	arg1	liposomes					1407:1415	GHK-modified liposomes	1394:1415	GHK-modified liposomes	1394:1415	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	1	66	theme	cellular	149:156	arg1	responses					158:166	cellular responses	149:166	cellular responses	149:166	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	2	67	theme	other	295:299	arg1	factors					311:317	other bioactive factors	295:317	other bioactive factors	295:317	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	12	68	theme	advanced	1934:1941	arg1	liposomes					1874:1882	The composite liposomes	1860:1882	The composite liposomes with GHK-heparin covering	1860:1908	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	12	68	theme	advanced	1934:1941	arg1	formulation					1953:1963	an advanced GHK-based formulation	1931:1963	an advanced GHK-based formulation for therapeutic and cosmeceutical applications	1931:2010	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	11	69	theme	cell-surface	1709:1720	arg1	glycosaminoglycans					1722:1739	cell-surface glycosaminoglycans	1709:1739	cell-surface glycosaminoglycans	1709:1739	Together, the results support that cell-surface glycosaminoglycans can be involved in GHK-mediated liposomal delivery, which can be further greatly enhanced by association with heparin.
37144381	5	70	theme	specific	851:858	arg1	manner					860:865	a specific manner	849:865	a specific manner	849:865	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	4	71	dep	In	608:609	arg1	situ					611:614	situ	611:614	situ	611:614	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	12	72	theme	covering	1901:1908	arg1	GHK-heparin					1889:1899	GHK-heparin covering	1889:1908	GHK-heparin covering	1889:1908	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	9	73	theme	composite	1448:1456	arg1	liposomes					1458:1466	the composite liposomes	1444:1466	the composite liposomes	1444:1466	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	6	74	theme	DLS	1098:1100	arg1	technique					1102:1110	the DLS technique	1094:1110	the DLS technique	1094:1110	The results provide a useful tool for screening of bio-interactions of synthetic peptide-presenting liposomes by the DLS technique.
37144381	1	75	theme	Small	86:90	arg1	alternatives					182:193	promising alternatives	172:193	promising alternatives to full-length therapeutic proteins	172:229	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	1	75	theme	Small	86:90	arg1	peptides					104:111	Small biospecific peptides	86:111	Small biospecific peptides with defined chemical structure and cellular responses	86:166	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	12	76	theme	composite	1864:1872	arg1	liposomes					1874:1882	The composite liposomes	1860:1882	The composite liposomes with GHK-heparin covering	1860:1908	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	12	76	theme	composite	1864:1872	arg1	formulation					1953:1963	an advanced GHK-based formulation	1931:1963	an advanced GHK-based formulation for therapeutic and cosmeceutical applications	1931:2010	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	3	77	theme	ECM-derived	489:499	arg1	peptide					505:511	ECM-derived GHK peptide	489:511	ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	489:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	9	78	theme	highest	1482:1488	arg1	activity					1507:1514	the highest cell-penetrating activity	1478:1514	the highest cell-penetrating activity	1478:1514	The heparin component significantly promoted the accumulation of GHK-modified liposomes in 3T3 fibroblasts so that the composite liposomes exhibited the highest cell-penetrating activity.
37144381	10	79	theme	oxidative	1645:1653	arg1	conditions					1662:1671	oxidative stress conditions	1645:1671	oxidative stress conditions	1645:1671	Furthermore, the latter formulation stimulated cell proliferation and strongly inhibited ROS production and GSH depletion under oxidative stress conditions.
37144381	3	80	theme	formulations	473:484	arg1	development					444:454	the development	440:454	the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	440:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	0	81	theme	peptide-heparin	18:32	arg1	interactions					34:45	GHK peptide-heparin interactions	14:45	GHK peptide-heparin interactions	14:45	Evaluation of GHK peptide-heparin interactions in multifunctional liposomal covering.
37144381	1	82	theme	defined	118:124	arg1	structure					135:143	defined chemical structure	118:143	defined chemical structure	118:143	Small biospecific peptides with defined chemical structure and cellular responses are promising alternatives to full-length therapeutic proteins.
37144381	4	83	theme	GHK	646:648	arg1	derivative					650:659	membranotropic GHK derivative	631:659	membranotropic GHK derivative	631:659	In situ association of membranotropic GHK derivative with unilamellar liposomes was performed to prepare GHK-modified liposomes with defined properties.
37144381	8	84	theme	resulting	1217:1225	arg1	liposomes					1237:1245	The resulting composite liposomes	1213:1245	The resulting composite liposomes	1213:1245	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	3	85	theme	new	459:461	arg1	formulations					473:484	new liposomal formulations	459:484	new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	459:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	3	86	theme	peptide	505:511	arg1	formulations					473:484	new liposomal formulations	459:484	new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	459:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	5	87	theme	other	879:883	arg1	polysaccharides					885:899	other polysaccharides	879:899	other polysaccharides	879:899	According to DLS, the GHK component on the liposomal surface interacted with heparin in a specific manner compared to other polysaccharides and RGD counterpart, whereas ITC analysis of such interactions was complicated.
37144381	12	88	with	liposomes	1874:1882	arg1	GHK-heparin					1889:1899	GHK-heparin covering	1889:1908	GHK-heparin covering	1889:1908	The composite liposomes with GHK-heparin covering can be considered as an advanced GHK-based formulation for therapeutic and cosmeceutical applications.
37144381	2	89	theme	peptides	256:263	arg1	Identification					232:245	Identification	232:245	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets	232:352	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	2	90	theme	current	385:391	arg1	research					407:414	current drug delivery research	385:414	current drug delivery research	385:414	Identification of these peptides solely or in combination with other bioactive factors and determination of their targets are of substantial interest in current drug delivery research.
37144381	8	91	theme	low	1257:1259	arg1	dispersity					1266:1275	low size dispersity	1257:1275	low size dispersity	1257:1275	The resulting composite liposomes possessed low size dispersity, increased anionic charge, and mechanical rigidity.
37144381	3	92	theme	multiple	527:534	arg1	activities					557:566	its multiple regeneration-related activities	523:566	its multiple regeneration-related activities	523:566	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
37144381	3	93	theme	cellular	590:597	arg1	targets					599:605	recognized cellular targets	579:605	ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets	489:605	This study is aimed at the development of new liposomal formulations of ECM-derived GHK peptide known for its multiple regeneration-related activities but poorly recognized cellular targets.
36447160	6	0	theme	composition	1064:1074	arg1	analysis					1076:1083	Cell wall composition analysis	1054:1083	Cell wall composition analysis	1054:1083	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	10	1	theme	several	1868:1874	arg1	genes					1876:1880	several genes	1868:1880	several genes	1868:1880	Furthermore, several genes positively or negatively regulate trichome development.
36447160	2	2	theme	trichomes	341:349	arg1	function					329:336	function	329:336	function	329:336	Currently, the morphogenesis and function of trichomes in desert plants are not well understood.
36447160	2	2	theme	trichomes	341:349	arg1	morphogenesis					311:323	morphogenesis	311:323	morphogenesis	311:323	Currently, the morphogenesis and function of trichomes in desert plants are not well understood.
36447160	1	3	theme	trichomes	241:249	arg1	evolution					228:236	the evolution	224:236	the evolution of trichomes	224:249	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	2	4	from	morphogenesis	311:323	arg1	plants					361:366	desert plants	354:366	desert plants	354:366	Currently, the morphogenesis and function of trichomes in desert plants are not well understood.
36447160	11	5	from	biogenesis	2005:2014	arg1	species					2077:2083	desert plant species	2064:2083	desert plant species	2064:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	10	6	theme	trichome	1916:1923	arg1	development					1925:1935	trichome development	1916:1935	trichome development	1916:1935	Furthermore, several genes positively or negatively regulate trichome development.
36447160	11	7	theme	biogenesis	2005:2014	arg1	further understanding					1976:1996	our further understanding	1972:1996	our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species	1972:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	2	8	from	function	329:336	arg1	plants					361:366	desert plants	354:366	desert plants	354:366	Currently, the morphogenesis and function of trichomes in desert plants are not well understood.
36447160	11	9	theme	plant	2034:2038	arg1	structures					2050:2059	plant accessory structures	2034:2059	plant accessory structures	2034:2059	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	8	10	theme	of cell	1705:1711	arg1	rounds					1698:1703	two to three rounds	1685:1703	two to three rounds of cell division	1685:1720	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	6	11	theme	methyl-esterified	1141:1157	arg1	pectins					1183:1189	heavily methyl-esterified and fully de-esterified pectins	1133:1189	heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall)	1133:1239	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	2	12	dep	morphogenesis	311:323	arg1	the					307:309	the	307:309	the	307:309	Currently, the morphogenesis and function of trichomes in desert plants are not well understood.
36447160	11	13	theme	adaptation	2020:2029	arg1	further understanding					1976:1996	our further understanding	1972:1996	our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species	1972:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	1	14	theme	various	197:203	arg1	protrusions					283:293	protective epidermal protrusions	262:293	protective epidermal protrusions	262:293	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	1	14	theme	various	197:203	arg1	adaptions					205:213	various adaptions	197:213	various adaptions	197:213	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	1	14	theme	various	197:203	arg1	evolution					228:236	the evolution	224:236	the evolution of trichomes	224:249	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	11	15	theme	accessory	2040:2048	arg1	structures					2050:2059	plant accessory structures	2034:2059	plant accessory structures	2034:2059	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	6	16	theme	primary	1227:1233	arg1	wall					1235:1238	the primary wall	1223:1238	the primary wall	1223:1238	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	4	17	theme	ferganica	682:690	arg1	composition					664:674	cell wall composition	654:674	cell wall composition	654:674	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	4	17	theme	ferganica	682:690	arg1	morphogenesis					636:648	morphogenesis	636:648	morphogenesis	636:648	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	8	18	contain	has	1630:1632	arg2	trichomes					1662:1670	multicellular, non-branched trichomes	1634:1670	multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress	1634:1755	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	8	18	contain	has	1630:1632	arg1	CONCLUSIONS					1605:1615	CONCLUSIONS	1605:1615	CONCLUSIONS S. ferganica	1605:1628	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	8	19	theme	abiotic	1742:1748	arg1	stress					1750:1755	abiotic stress	1742:1755	abiotic stress	1742:1755	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	5	20	theme	epidermal	885:893	arg1	primordium					895:904	the epidermal primordium	881:904	the epidermal primordium	881:904	RESULTS The trichomes of S. ferganica were initiated from the epidermal primordium, followed by two to three rounds of cell division to form a multicellular trichome, while some genes associated with them were positively involved.
36447160	7	21	theme	mesophyll	1568:1576	arg1	cells					1578:1582	the mesophyll cells	1564:1582	the mesophyll cells	1564:1582	Moreover, trichome development was affected by abiotic stress, and might accumulate salt from the mesophyll cells and secrete outside.
36447160	11	22	theme	plant	2071:2075	arg1	species					2077:2083	desert plant species	2064:2083	desert plant species	2064:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	5	23	dep	three	926:930	arg1	to					923:924	to	923:924	to	923:924	RESULTS The trichomes of S. ferganica were initiated from the epidermal primordium, followed by two to three rounds of cell division to form a multicellular trichome, while some genes associated with them were positively involved.
36447160	4	24	from	function	778:785	arg1	accumulation					795:806	salt accumulation	790:806	salt accumulation of the leaves	790:820	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	11	25	dep	biogenesis	2005:2014	arg1	the					2001:2003	the	2001:2003	the	2001:2003	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	4	26	theme	wall	659:662	arg1	composition					664:674	cell wall composition	654:674	cell wall composition	654:674	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	6	27	theme	whole	1349:1353	arg1	period					1369:1374	the whole developmental period	1345:1374	the whole developmental period	1345:1374	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	0	28	theme	cell	18:21	arg1	composition					28:38	cell wall composition	18:38	cell wall composition	18:38	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	7	29	theme	might accumulate	1537:1552	arg1	salt					1554:1557	might accumulate salt	1537:1557	might accumulate salt	1537:1557	Moreover, trichome development was affected by abiotic stress, and might accumulate salt from the mesophyll cells and secrete outside.
36447160	8	30	dep	S.	1617:1618	arg1	ferganica					1620:1628	ferganica	1620:1628	ferganica	1620:1628	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	6	31	from	maturation	1199:1208	arg1	wall					1235:1238	the primary wall	1223:1238	the primary wall	1223:1238	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	4	32	from	expression	750:759	arg1	accumulation					795:806	salt accumulation	790:806	salt accumulation of the leaves	790:820	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	0	33	dep	Salsola	105:111	arg1	Morphogenesis					0:12	Morphogenesis	0:12	Morphogenesis	0:12	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	0	33	dep	Salsola	105:111	arg1	ferganica					113:121	Salsola ferganica	105:121	Salsola ferganica	105:121	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	0	33	dep	Salsola	105:111	arg1	composition					28:38	cell wall composition	18:38	cell wall composition	18:38	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	0	33	dep	Salsola	105:111	arg1	function					63:70	their function	57:70	their function in response to salt	57:90	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	0	34	from	Morphogenesis	0:12	arg1	halophyte					95:103	halophyte	95:103	halophyte	95:103	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	4	35	theme	related	737:743	arg1	expression					750:759	related gene expression	737:759	related gene expression	737:759	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	7	36	theme	abiotic	1517:1523	arg1	stress					1525:1530	abiotic stress	1517:1530	abiotic stress	1517:1530	Moreover, trichome development was affected by abiotic stress, and might accumulate salt from the mesophyll cells and secrete outside.
36447160	4	37	from	cotton	729:734	arg1	accumulation					795:806	salt accumulation	790:806	salt accumulation of the leaves	790:820	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	0	38	from	composition	28:38	arg1	halophyte					95:103	halophyte	95:103	halophyte	95:103	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	5	39	dep	initiated	866:874	arg1	followed					907:914	followed	907:914	followed by two to three rounds	907:937	RESULTS The trichomes of S. ferganica were initiated from the epidermal primordium, followed by two to three rounds of cell division to form a multicellular trichome, while some genes associated with them were positively involved.
36447160	4	40	from	thaliana	716:723	arg1	accumulation					795:806	salt accumulation	790:806	salt accumulation of the leaves	790:820	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	9	41	theme	cell	1777:1780	arg1	composition					1787:1797	a unique cell wall composition	1768:1797	a unique cell wall composition which is different from that of Arabidopsis and cotton	1768:1852	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	9	41	theme	cell	1777:1780	arg1	different					1808:1816	different	1808:1816	different	1808:1816	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	4	42	dep	the	811:813	arg1	leaves					815:820	leaves	815:820	leaves	815:820	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	1	43	theme	epidermal	273:281	arg1	protrusions					283:293	protective epidermal protrusions	262:293	protective epidermal protrusions	262:293	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	1	43	theme	epidermal	273:281	arg1	adaptions					205:213	various adaptions	197:213	various adaptions	197:213	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	1	43	theme	epidermal	273:281	arg1	evolution					228:236	the evolution	224:236	the evolution of trichomes	224:249	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	3	44	dep	true	501:504	arg1	leaves					506:511	leaves	506:511	leaves	506:511	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	4	45	theme	salt	790:793	arg1	accumulation					795:806	salt accumulation	790:806	salt accumulation of the leaves	790:820	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	7	46	theme	trichome	1480:1487	arg1	development					1489:1499	trichome development	1480:1499	trichome development	1480:1499	Moreover, trichome development was affected by abiotic stress, and might accumulate salt from the mesophyll cells and secrete outside.
36447160	3	47	theme	seedling	470:477	arg1	stage					479:483	the seedling stage	466:483	the seedling stage	466:483	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	0	48	theme	trichomes	43:51	arg1	Morphogenesis					0:12	Morphogenesis	0:12	Morphogenesis	0:12	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	0	48	theme	trichomes	43:51	arg1	function					63:70	their function	57:70	their function in response to salt	57:90	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	0	48	theme	trichomes	43:51	arg1	composition					28:38	cell wall composition	18:38	cell wall composition	18:38	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	6	49	theme	Arabidopsis	1446:1456	arg1	trichomes					1433:1441	trichomes	1433:1441	trichomes of Arabidopsis and cotton	1433:1467	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	11	50	from	further understanding	1976:1996	arg1	species					2077:2083	desert plant species	2064:2083	desert plant species	2064:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	6	51	theme	middle	1276:1281	arg1	stages					1283:1288	the mid-early and middle stages	1258:1288	the mid-early and middle stages	1258:1288	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	52	from	xyloglucans	1242:1252	arg1	stages					1283:1288	the mid-early and middle stages	1258:1288	the mid-early and middle stages	1258:1288	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	53	theme	wall	1059:1062	arg1	analysis					1076:1083	Cell wall composition analysis	1054:1083	Cell wall composition analysis	1054:1083	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	54	theme	mid-early	1262:1270	arg1	stages					1283:1288	the mid-early and middle stages	1258:1288	the mid-early and middle stages	1258:1288	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	9	55	contain	have	1763:1766	arg2	different					1808:1816	different	1808:1816	different	1808:1816	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	9	55	contain	have	1763:1766	arg1	They					1758:1761	They	1758:1761	They	1758:1761	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	9	55	contain	have	1763:1766	arg2	composition					1787:1797	a unique cell wall composition	1768:1797	a unique cell wall composition which is different from that of Arabidopsis and cotton	1768:1852	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	4	56	theme	the	811:813	arg1	accumulation					795:806	salt accumulation	790:806	salt accumulation of the leaves	790:820	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	6	57	theme	de-esterified	1169:1181	arg1	pectins					1183:1189	heavily methyl-esterified and fully de-esterified pectins	1133:1189	heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall)	1133:1239	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	3	58	theme	thick	539:543	arg1	trichomes					545:553	long and thick trichomes	530:553	long and thick trichomes	530:553	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	4	59	from	trichomes	623:631	arg1	composition					664:674	cell wall composition	654:674	cell wall composition	654:674	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	4	59	from	trichomes	623:631	arg1	morphogenesis					636:648	morphogenesis	636:648	morphogenesis	636:648	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	1	60	theme	desert	178:183	arg1	plants					185:190	desert plants	178:190	desert plants	178:190	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	3	61	theme	long	530:533	arg1	trichomes					545:553	long and thick trichomes	530:553	long and thick trichomes	530:553	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	11	62	theme	desert	2064:2069	arg1	species					2077:2083	desert plant species	2064:2083	desert plant species	2064:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	1	63	theme	environmental	152:164	arg1	conditions					166:175	harsh environmental conditions	146:175	harsh environmental conditions	146:175	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	6	64	theme	secondary	1307:1315	arg1	wall					1317:1320	the secondary wall	1303:1320	the secondary wall	1303:1320	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	65	theme	cotton	1462:1467	arg1	trichomes					1433:1441	trichomes	1433:1441	trichomes of Arabidopsis and cotton	1433:1467	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	11	66	theme	structures	2050:2059	arg1	adaptation					2020:2029	adaptation	2020:2029	adaptation	2020:2029	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	11	66	theme	structures	2050:2059	arg1	biogenesis					2005:2014	biogenesis	2005:2014	biogenesis	2005:2014	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	0	67	from	function	63:70	arg1	halophyte					95:103	halophyte	95:103	halophyte	95:103	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	8	68	dep	multicellular	1634:1646	arg1	non-branched					1649:1660	non-branched	1649:1660	non-branched	1649:1660	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	11	69	from	species	2077:2083	arg1	further understanding					1976:1996	our further understanding	1972:1996	our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species	1972:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	6	70	theme	developmental	1355:1367	arg1	period					1369:1374	the whole developmental period	1345:1374	the whole developmental period	1345:1374	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	3	71	theme	habitat	575:581	arg1	conditions					583:592	habitat conditions	575:592	habitat conditions	575:592	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	5	72	theme	ferganica	851:859	arg1	trichomes					835:843	The trichomes	831:843	The trichomes of S. ferganica	831:859	RESULTS The trichomes of S. ferganica were initiated from the epidermal primordium, followed by two to three rounds of cell division to form a multicellular trichome, while some genes associated with them were positively involved.
36447160	8	73	theme	S.	1617:1618	arg1	CONCLUSIONS					1605:1615	CONCLUSIONS	1605:1615	CONCLUSIONS S. ferganica	1605:1628	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	4	74	theme	cell	654:657	arg1	composition					664:674	cell wall composition	654:674	cell wall composition	654:674	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	1	75	theme	harsh	146:150	arg1	conditions					166:175	harsh environmental conditions	146:175	harsh environmental conditions	146:175	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	7	76	from	cells	1578:1582	arg1	salt					1554:1557	might accumulate salt	1537:1557	might accumulate salt	1537:1557	Moreover, trichome development was affected by abiotic stress, and might accumulate salt from the mesophyll cells and secrete outside.
36447160	9	77	from	that	1823:1826	arg1	composition					1787:1797	a unique cell wall composition	1768:1797	a unique cell wall composition which is different from that of Arabidopsis and cotton	1768:1852	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	9	77	from	that	1823:1826	arg1	different					1808:1816	different	1808:1816	different	1808:1816	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	8	78	dep	of cell	1705:1711	arg1	division					1713:1720	division	1713:1720	division	1713:1720	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	6	79	from	pectins	1183:1189	arg1	stages					1283:1288	the mid-early and middle stages	1258:1288	the mid-early and middle stages	1258:1288	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	0	80	dep	salt	87:90	arg1	response					75:82	response	75:82	response	75:82	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	6	81	theme	Cell	1054:1057	arg1	analysis					1076:1083	Cell wall composition analysis	1054:1083	Cell wall composition analysis	1054:1083	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	8	82	dep	three	1692:1696	arg1	to					1689:1690	to	1689:1690	to	1689:1690	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	4	83	theme	gene	745:748	arg1	expression					750:759	related gene expression	737:759	related gene expression	737:759	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	6	84	from	extensin	1328:1335	arg1	stages					1283:1288	the mid-early and middle stages	1258:1288	the mid-early and middle stages	1258:1288	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	9	85	theme	unique	1770:1775	arg1	composition					1787:1797	a unique cell wall composition	1768:1797	a unique cell wall composition which is different from that of Arabidopsis and cotton	1768:1852	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	9	85	theme	unique	1770:1775	arg1	different					1808:1816	different	1808:1816	different	1808:1816	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	4	86	theme	Arabidopsis	704:714	arg1	thaliana					716:723	Arabidopsis thaliana	704:723	Arabidopsis thaliana	704:723	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	3	87	theme	annual	417:422	arg1	halophyte					424:432	an annual halophyte	414:432	an annual halophyte distributed in cold deserts	414:460	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	3	87	theme	annual	417:422	arg1	ferganica					401:409	Salsola ferganica	393:409	Salsola ferganica	393:409	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	0	88	theme	wall	23:26	arg1	composition					28:38	cell wall composition	18:38	cell wall composition	18:38	Morphogenesis and cell wall composition of trichomes and their function in response to salt in halophyte Salsola ferganica.
36447160	1	89	theme	protective	262:271	arg1	protrusions					283:293	protective epidermal protrusions	262:293	protective epidermal protrusions	262:293	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	1	89	theme	protective	262:271	arg1	adaptions					205:213	various adaptions	197:213	various adaptions	197:213	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	1	89	theme	protective	262:271	arg1	evolution					228:236	the evolution	224:236	the evolution of trichomes	224:249	BACKGROUND To survive harsh environmental conditions, desert plants show various adaptions, such as the evolution of trichomes, which are protective epidermal protrusions.
36447160	6	90	located	found	1424:1428	arg2	those					1418:1422	those	1418:1422	those	1418:1422	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	90	located	found	1424:1428	arg1	trichomes					1433:1441	trichomes	1433:1441	trichomes of Arabidopsis and cotton	1433:1467	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	9	91	theme	wall	1782:1785	arg1	composition					1787:1797	a unique cell wall composition	1768:1797	a unique cell wall composition which is different from that of Arabidopsis and cotton	1768:1852	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	9	91	theme	wall	1782:1785	arg1	different					1808:1816	different	1808:1816	different	1808:1816	They have a unique cell wall composition which is different from that of Arabidopsis and cotton.
36447160	5	92	theme	of cell	939:945	arg1	division					947:954	of cell division	939:954	of cell division	939:954	RESULTS The trichomes of S. ferganica were initiated from the epidermal primordium, followed by two to three rounds of cell division to form a multicellular trichome, while some genes associated with them were positively involved.
36447160	3	93	theme	cold	449:452	arg1	deserts					454:460	cold deserts	449:460	cold deserts	449:460	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	6	94	from	stages	1283:1288	arg1	wall					1317:1320	the secondary wall	1303:1320	the secondary wall	1303:1320	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	3	95	theme	Salsola	393:399	arg1	halophyte					424:432	an annual halophyte	414:432	an annual halophyte distributed in cold deserts	414:460	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	3	95	theme	Salsola	393:399	arg1	ferganica					401:409	Salsola ferganica	393:409	Salsola ferganica	393:409	Salsola ferganica is an annual halophyte distributed in cold deserts; at the seedling stage, its rod-shaped true leaves are covered with long and thick trichomes and are affected by habitat conditions.
36447160	6	96	from	those	1418:1422	arg1	different					1403:1411	different	1403:1411	different	1403:1411	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	97	theme	different	1097:1105	arg1	extensin					1328:1335	extensin	1328:1335	extensin (during the whole developmental period)	1328:1375	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	97	theme	different	1097:1105	arg1	pectins					1183:1189	heavily methyl-esterified and fully de-esterified pectins	1133:1189	heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall)	1133:1239	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	97	theme	different	1097:1105	arg1	polysaccharides					1107:1121	different polysaccharides	1097:1121	different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period)	1097:1375	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	6	97	theme	different	1097:1105	arg1	xyloglucans					1242:1252	xyloglucans	1242:1252	xyloglucans (in the mid-early and middle stages, probably in the secondary wall)	1242:1321	Cell wall composition analysis showed that different polysaccharides including heavily methyl-esterified and fully de-esterified pectins (before maturation, probably in the primary wall), xyloglucans (in the mid-early and middle stages, probably in the secondary wall), and extensin (during the whole developmental period) were detected, which were different from those found in trichomes of Arabidopsis and cotton.
36447160	11	98	from	adaptation	2020:2029	arg1	species					2077:2083	desert plant species	2064:2083	desert plant species	2064:2083	Our findings should contribute to our further understanding of the biogenesis and adaptation of plant accessory structures in desert plant species.
36447160	5	99	dep	RESULTS	823:829	arg1	initiated					866:874	initiated	866:874	initiated	866:874	RESULTS The trichomes of S. ferganica were initiated from the epidermal primordium, followed by two to three rounds of cell division to form a multicellular trichome, while some genes associated with them were positively involved.
36447160	8	100	theme	multicellular	1634:1646	arg1	trichomes					1662:1670	multicellular, non-branched trichomes	1634:1670	multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress	1634:1755	CONCLUSIONS S. ferganica has multicellular, non-branched trichomes that undergo two to three rounds of cell division and are affected by abiotic stress.
36447160	5	101	theme	a multicellular	964:978	arg1	trichome					980:987	a multicellular trichome	964:987	a multicellular trichome	964:987	RESULTS The trichomes of S. ferganica were initiated from the epidermal primordium, followed by two to three rounds of cell division to form a multicellular trichome, while some genes associated with them were positively involved.
36447160	4	102	theme	preliminary	766:776	arg1	function					778:785	preliminary function	766:785	preliminary function	766:785	Therefore, we evaluated the trichomes on morphogenesis and cell wall composition of S. ferganica compared to Arabidopsis thaliana and cotton, related gene expression, and preliminary function in salt accumulation of the leaves.
36447160	2	103	theme	desert	354:359	arg1	plants					361:366	desert plants	354:366	desert plants	354:366	Currently, the morphogenesis and function of trichomes in desert plants are not well understood.
36157541	4	0	theme	labeling-based	1010:1023	arg1	quantitation					1025:1036	iTRAQ labeling-based quantitation	1004:1036	iTRAQ labeling-based quantitation	1004:1036	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	2	1	theme	N-linked	279:286	arg1	profile					303:309	the N-linked glycoproteomic profile	275:309	the N-linked glycoproteomic profile	275:309	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	6	2	theme	haptoglobin	1388:1398	arg1	up-regulation					1284:1296	the up-regulation	1280:1296	the up-regulation of total procathepsin D and high-mannose procathepsin D	1280:1352	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	2	theme	haptoglobin	1388:1398	arg1	down-regulation					1363:1377	the down-regulation	1359:1377	the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1359:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	3	theme	absolute	639:646	arg1	quantitation					704:715	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	0	4	link	N-linked	0:7	arg1	glycoproteomic					9:22	glycoproteomic	9:22	glycoproteomic	9:22	N-linked glycoproteomic profiling in esophageal squamous cell carcinoma.
36157541	3	5	from	electrophoresis	575:589	arg1	parallel					720:727	parallel	720:727	parallel	720:727	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	6	theme	relative	626:633	arg1	quantitation					704:715	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	7	7	theme	healthy	1683:1689	arg1	controls					1691:1698	healthy controls	1683:1698	healthy controls	1683:1698	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	2	8	theme	complementary	364:376	arg1	approaches					378:387	two complementary approaches	360:387	two complementary approaches	360:387	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	1	9	theme	sequencing	243:252	arg1	scope					226:230	the scope	222:230	the scope of genomic sequencing	222:252	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	3	10	link	N-linked	502:509	arg1	profiling					526:534	N-linked glycoproteomic profiling	502:534	N-linked glycoproteomic profiling in ESCC tissues	502:550	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	6	11	theme	fraction	1459:1466	arg1	up-regulation					1284:1296	the up-regulation	1280:1296	the up-regulation of total procathepsin D and high-mannose procathepsin D	1280:1352	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	11	theme	fraction	1459:1466	arg1	down-regulation					1363:1377	the down-regulation	1359:1377	the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1359:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	7	12	theme	clusterin	1542:1550	arg1	fractions					1529:1537	glycosylated fractions	1516:1537	glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1516:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	2	13	theme	glycoproteomic	288:301	arg1	profile					303:309	the N-linked glycoproteomic profile	275:309	the N-linked glycoproteomic profile	275:309	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	6	14	theme	GlcNAc/sialic	1429:1441	arg1	fraction					1459:1466	GlcNAc/sialic acid-containing fraction	1429:1466	GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1429:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	15	theme	mass	686:689	arg1	spectrometry					691:702	quantification (iTRAQ) labeling-based mass spectrometry	648:702	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	7	16	from	patients	1627:1634	arg1	fractions					1529:1537	glycosylated fractions	1516:1537	glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1516:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	7	17	theme	repeat	1592:1597	arg1	protein					1599:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	2	18	link	N-linked	279:286	arg1	profile					303:309	the N-linked glycoproteomic profile	275:309	the N-linked glycoproteomic profile	275:309	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	2	19	theme	squamous	325:332	arg1	ESCC					350:353	ESCC	350:353	ESCC	350:353	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	2	19	theme	squamous	325:332	arg1	carcinoma					339:347	esophageal squamous cell carcinoma	314:347	esophageal squamous cell carcinoma (ESCC)	314:354	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	1	20	theme	significant	183:193	arg1	insights					206:213	significant biological insights	183:213	significant biological insights	183:213	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	6	21	theme	high-mannose	1401:1412	arg1	clusterin					1414:1422	high-mannose clusterin	1401:1422	high-mannose clusterin	1401:1422	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	7	22	theme	glycosylated	1516:1527	arg1	fractions					1529:1537	glycosylated fractions	1516:1537	glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1516:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	4	23	theme	2-DE-based	815:824	arg1	quantitation					851:862	2-DE-based and iTRAQ labeling-based quantitation	815:862	RESULTS 2-DE-based and iTRAQ labeling-based quantitation	807:862	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	4	24	gly	glycoproteins	920:932	arg1	glycoproteins					920:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	7	25	gly	glycosylated	1516:1527	arg1	fractions					1529:1537	glycosylated fractions	1516:1537	glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1516:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	7	26	theme	protein	1599:1605	arg1	fractions					1529:1537	glycosylated fractions	1516:1537	glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1516:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	4	27	theme	expressed	901:909	arg1	glycoproteins					920:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	4	28	dep	RESULTS	807:813	arg1	quantitation					851:862	2-DE-based and iTRAQ labeling-based quantitation	815:862	RESULTS 2-DE-based and iTRAQ labeling-based quantitation	807:862	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	3	29	theme	multilectin	411:421	arg1	chromatography					432:445	tandem multilectin affinity chromatography	404:445	tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins	404:486	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	8	30	theme	N-linked	1758:1765	arg1	glycoproteome					1767:1779	the aberrant N-linked glycoproteome	1745:1779	the aberrant N-linked glycoproteome associated with ESCC	1745:1800	CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations.
36157541	5	31	theme	categories	1151:1160	arg1	compositions					1124:1135	the distinct compositions	1111:1135	the distinct compositions of functional categories between proteins and glycoproteins	1111:1195	Proteomaps showed the distinct compositions of functional categories between proteins and glycoproteins with differential expression associated with ESCC.
36157541	7	32	theme	proline-arginine-rich	1553:1573	arg1	protein					1599:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	3	33	theme	N-linked	465:472	arg1	glycoproteins					474:486	N-linked glycoproteins	465:486	N-linked glycoproteins	465:486	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	34	theme	glycoproteomic	511:524	arg1	profiling					526:534	N-linked glycoproteomic profiling	502:534	N-linked glycoproteomic profiling in ESCC tissues	502:550	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	6	35	theme	blot	1256:1259	arg1	analysis					1261:1268	Western blot analysis	1248:1268	Western blot analysis	1248:1268	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	7	36	theme	end	1575:1577	arg1	protein					1599:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	3	37	theme	two-dimensional	555:569	arg1	2-DE					592:595	2-DE	592:595	2-DE	592:595	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	37	theme	two-dimensional	555:569	arg1	electrophoresis					575:589	two-dimensional gel electrophoresis	555:589	two-dimensional gel electrophoresis (2-DE)-based	555:602	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	5	38	theme	distinct	1115:1122	arg1	compositions					1124:1135	the distinct compositions	1111:1135	the distinct compositions of functional categories between proteins and glycoproteins	1111:1195	Proteomaps showed the distinct compositions of functional categories between proteins and glycoproteins with differential expression associated with ESCC.
36157541	7	39	from	haptoglobin	1612:1622	arg1	patients					1627:1634	patients	1627:1634	patients with ESCC	1627:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	7	40	theme	fractions	1529:1537	arg1	higher					1662:1667	higher	1662:1667	higher	1662:1667	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	7	40	theme	fractions	1529:1537	arg1	levels					1506:1511	The serum levels	1496:1511	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1496:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	3	41	theme	candidate	756:764	arg1	biomarkers					779:788	candidate glycoprotein biomarkers	756:788	candidate glycoprotein biomarkers	756:788	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	0	42	theme	esophageal	37:46	arg1	carcinoma					62:70	esophageal squamous cell carcinoma	37:70	esophageal squamous cell carcinoma	37:70	N-linked glycoproteomic profiling in esophageal squamous cell carcinoma.
36157541	5	43	theme	differential	1202:1213	arg1	expression					1215:1224	differential expression	1202:1224	differential expression associated with ESCC	1202:1245	Proteomaps showed the distinct compositions of functional categories between proteins and glycoproteins with differential expression associated with ESCC.
36157541	5	44	gly	glycoproteins	1183:1195	arg1	glycoproteins					1183:1195	glycoproteins	1183:1195	glycoproteins	1183:1195	Proteomaps showed the distinct compositions of functional categories between proteins and glycoproteins with differential expression associated with ESCC.
36157541	0	45	theme	cell	57:60	arg1	carcinoma					62:70	esophageal squamous cell carcinoma	37:70	esophageal squamous cell carcinoma	37:70	N-linked glycoproteomic profiling in esophageal squamous cell carcinoma.
36157541	6	46	theme	high-mannose	1326:1337	arg1	D					1352:1352	high-mannose procathepsin D	1326:1352	high-mannose procathepsin D	1326:1352	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	8	47	theme	valuable	1819:1826	arg1	insights					1731:1738	insights	1731:1738	insights	1731:1738	CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations.
36157541	8	47	theme	valuable	1819:1826	arg1	resource					1828:1835	a valuable resource	1817:1835	a valuable resource for future investigations	1817:1861	CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations.
36157541	3	48	theme	Western	793:799	arg1	blot					801:804	Western blot	793:804	Western blot	793:804	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	6	49	theme	D	1320:1320	arg1	up-regulation					1284:1296	the up-regulation	1280:1296	the up-regulation of total procathepsin D and high-mannose procathepsin D	1280:1352	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	49	theme	D	1320:1320	arg1	down-regulation					1363:1377	the down-regulation	1359:1377	the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1359:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	50	theme	biomarkers	779:788	arg1	validation					742:751	validation	742:751	validation of candidate glycoprotein biomarkers	742:788	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	51	from	profiling	526:534	arg1	tissues					544:550	ESCC tissues	539:550	ESCC tissues	539:550	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	52	theme	isobaric	608:615	arg1	tags					617:620	isobaric tags	608:620	isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel	608:727	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	7	53	theme	serum	1500:1504	arg1	higher					1662:1667	higher	1662:1667	higher	1662:1667	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	7	53	theme	serum	1500:1504	arg1	levels					1506:1511	The serum levels	1496:1511	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1496:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	6	54	theme	total	1301:1305	arg1	D					1320:1320	total procathepsin D	1301:1320	total procathepsin D	1301:1320	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	55	gly	glycoprotein	766:777	arg1	glycoprotein					766:777	candidate glycoprotein biomarkers	756:788	candidate glycoprotein biomarkers	756:788	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	56	theme	-based	597:602	arg1	2-DE					592:595	2-DE	592:595	2-DE	592:595	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	56	theme	-based	597:602	arg1	electrophoresis					575:589	two-dimensional gel electrophoresis	555:589	two-dimensional gel electrophoresis (2-DE)-based	555:602	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	4	57	theme	iTRAQ	1004:1008	arg1	quantitation					1025:1036	iTRAQ labeling-based quantitation	1004:1036	iTRAQ labeling-based quantitation	1004:1036	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	8	58	link	N-linked	1758:1765	arg1	glycoproteome					1767:1779	the aberrant N-linked glycoproteome	1745:1779	the aberrant N-linked glycoproteome associated with ESCC	1745:1800	CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations.
36157541	4	59	theme	quantitation	1025:1036	arg1	outperformance					986:999	the outperformance	982:999	the outperformance of iTRAQ labeling-based quantitation	982:1036	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	6	60	theme	total	1382:1386	arg1	haptoglobin					1388:1398	total haptoglobin	1382:1398	total haptoglobin	1382:1398	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	61	theme	ESCC	1482:1485	arg1	tissues					1487:1493	ESCC tissues	1482:1493	ESCC tissues	1482:1493	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	62	from	tags	617:620	arg1	parallel					720:727	parallel	720:727	parallel	720:727	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	6	63	theme	14-3-3ζ	1471:1477	arg1	clusterin					1414:1422	high-mannose clusterin	1401:1422	high-mannose clusterin	1401:1422	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	63	theme	14-3-3ζ	1471:1477	arg1	fraction					1459:1466	GlcNAc/sialic acid-containing fraction	1429:1466	GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1429:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	63	theme	14-3-3ζ	1471:1477	arg1	haptoglobin					1388:1398	total haptoglobin	1382:1398	total haptoglobin	1382:1398	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	64	theme	iTRAQ	664:668	arg1	spectrometry					691:702	quantification (iTRAQ) labeling-based mass spectrometry	648:702	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	1	65	theme	genomic	235:241	arg1	sequencing					243:252	genomic sequencing	235:252	genomic sequencing	235:252	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	3	66	theme	quantification	648:661	arg1	spectrometry					691:702	quantification (iTRAQ) labeling-based mass spectrometry	648:702	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	6	67	theme	D	1352:1352	arg1	up-regulation					1284:1296	the up-regulation	1280:1296	the up-regulation of total procathepsin D and high-mannose procathepsin D	1280:1352	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	67	theme	D	1352:1352	arg1	down-regulation					1363:1377	the down-regulation	1359:1377	the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1359:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	4	68	link	N-linked	911:918	arg1	glycoproteins					920:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	3	69	theme	spectrometry	691:702	arg1	quantitation					704:715	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	4	70	theme	approaches	1081:1090	arg1	complementarity					1052:1066	complementarity	1052:1066	complementarity	1052:1066	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	4	70	theme	approaches	1081:1090	arg1	2-DE					1043:1046	2-DE	1043:1046	2-DE	1043:1046	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	6	71	theme	acid-containing	1443:1457	arg1	fraction					1459:1466	GlcNAc/sialic acid-containing fraction	1429:1466	GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1429:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	72	theme	labeling-based	671:684	arg1	spectrometry					691:702	quantification (iTRAQ) labeling-based mass spectrometry	648:702	relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation	626:715	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	1	73	theme	post-translational	140:157	arg1	modifications					159:171	post-translational modifications	140:171	post-translational modifications providing significant biological insights beyond the scope of genomic sequencing	140:252	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	2	74	theme	cell	334:337	arg1	ESCC					350:353	ESCC	350:353	ESCC	350:353	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	2	74	theme	cell	334:337	arg1	carcinoma					339:347	esophageal squamous cell carcinoma	314:347	esophageal squamous cell carcinoma (ESCC)	314:354	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	4	75	from	15	954:955	arg1	common					960:965	common	960:965	common	960:965	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	6	76	theme	clusterin	1414:1422	arg1	up-regulation					1284:1296	the up-regulation	1280:1296	the up-regulation of total procathepsin D and high-mannose procathepsin D	1280:1352	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	6	76	theme	clusterin	1414:1422	arg1	down-regulation					1363:1377	the down-regulation	1359:1377	the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ	1359:1477	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	4	77	theme	labeling-based	836:849	arg1	quantitation					851:862	2-DE-based and iTRAQ labeling-based quantitation	815:862	RESULTS 2-DE-based and iTRAQ labeling-based quantitation	807:862	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	2	78	theme	esophageal	314:323	arg1	ESCC					350:353	ESCC	350:353	ESCC	350:353	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	2	78	theme	esophageal	314:323	arg1	carcinoma					339:347	esophageal squamous cell carcinoma	314:347	esophageal squamous cell carcinoma (ESCC)	314:354	AIM To characterize the N-linked glycoproteomic profile in esophageal squamous cell carcinoma (ESCC) via two complementary approaches.
36157541	1	79	theme	biological	195:204	arg1	insights					206:213	significant biological insights	183:213	significant biological insights	183:213	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	3	80	theme	tandem	404:409	arg1	chromatography					432:445	tandem multilectin affinity chromatography	404:445	tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins	404:486	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	7	81	theme	leucine-rich	1579:1590	arg1	protein					1599:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	proline-arginine-rich end leucine-rich repeat protein	1553:1605	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	8	82	theme	aberrant	1749:1756	arg1	glycoproteome					1767:1779	the aberrant N-linked glycoproteome	1745:1779	the aberrant N-linked glycoproteome associated with ESCC	1745:1800	CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations.
36157541	1	83	theme	BACKGROUND	73:82	arg1	proteomics					108:117	BACKGROUND Mass spectrometry-based proteomics	73:117	BACKGROUND Mass spectrometry-based proteomics	73:117	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	3	84	gly	glycoproteins	474:486	arg1	glycoproteins					474:486	N-linked glycoproteins	465:486	N-linked glycoproteins	465:486	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	1	85	theme	Mass	84:87	arg1	proteomics					108:117	BACKGROUND Mass spectrometry-based proteomics	73:117	BACKGROUND Mass spectrometry-based proteomics	73:117	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	3	86	theme	affinity	423:430	arg1	chromatography					432:445	tandem multilectin affinity chromatography	404:445	tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins	404:486	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	4	87	theme	N-linked	911:918	arg1	glycoproteins					920:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	24 and 402 differentially expressed N-linked glycoproteins	875:932	RESULTS 2-DE-based and iTRAQ labeling-based quantitation identified 24 and 402 differentially expressed N-linked glycoproteins, respectively, with 15 in common, demonstrating the outperformance of iTRAQ labeling-based quantitation over 2-DE and complementarity of these two approaches.
36157541	3	88	theme	ESCC	539:542	arg1	tissues					544:550	ESCC tissues	539:550	ESCC tissues	539:550	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	7	89	with	patients	1627:1634	arg1	ESCC					1641:1644	ESCC	1641:1644	ESCC	1641:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	7	90	from	protein	1599:1605	arg1	patients					1627:1634	patients	1627:1634	patients with ESCC	1627:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	3	91	theme	glycoproteins	474:486	arg1	enrichment					451:460	enrichment	451:460	enrichment of N-linked glycoproteins	451:486	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	7	92	theme	haptoglobin	1612:1622	arg1	fractions					1529:1537	glycosylated fractions	1516:1537	glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC	1516:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	6	93	theme	Western	1248:1254	arg1	analysis					1261:1268	Western blot analysis	1248:1268	Western blot analysis	1248:1268	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	5	94	theme	functional	1140:1149	arg1	categories					1151:1160	functional categories	1140:1160	functional categories	1140:1160	Proteomaps showed the distinct compositions of functional categories between proteins and glycoproteins with differential expression associated with ESCC.
36157541	3	95	theme	gel	571:573	arg1	2-DE					592:595	2-DE	592:595	2-DE	592:595	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	3	95	theme	gel	571:573	arg1	electrophoresis					575:589	two-dimensional gel electrophoresis	555:589	two-dimensional gel electrophoresis (2-DE)-based	555:602	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	0	96	theme	squamous	48:55	arg1	carcinoma					62:70	esophageal squamous cell carcinoma	37:70	esophageal squamous cell carcinoma	37:70	N-linked glycoproteomic profiling in esophageal squamous cell carcinoma.
36157541	8	97	dep	CONCLUSION	1701:1710	arg1	provides					1722:1729	provides	1722:1729	provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations	1722:1861	CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations.
36157541	3	98	theme	N-linked	502:509	arg1	profiling					526:534	N-linked glycoproteomic profiling	502:534	N-linked glycoproteomic profiling in ESCC tissues	502:550	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	6	99	theme	procathepsin	1339:1350	arg1	D					1352:1352	high-mannose procathepsin D	1326:1352	high-mannose procathepsin D	1326:1352	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	7	100	from	fractions	1529:1537	arg1	patients					1627:1634	patients	1627:1634	patients with ESCC	1627:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	8	101	theme	future	1841:1846	arg1	investigations					1848:1861	future investigations	1841:1861	future investigations	1841:1861	CONCLUSION Our study provides insights into the aberrant N-linked glycoproteome associated with ESCC, which will be a valuable resource for future investigations.
36157541	1	102	theme	spectrometry-based	89:106	arg1	proteomics					108:117	BACKGROUND Mass spectrometry-based proteomics	73:117	BACKGROUND Mass spectrometry-based proteomics	73:117	BACKGROUND Mass spectrometry-based proteomics and glycomics reveal post-translational modifications providing significant biological insights beyond the scope of genomic sequencing.
36157541	3	103	theme	glycoprotein	766:777	arg1	biomarkers					779:788	candidate glycoprotein biomarkers	756:788	candidate glycoprotein biomarkers	756:788	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36157541	7	104	from	clusterin	1542:1550	arg1	patients					1627:1634	patients	1627:1634	patients with ESCC	1627:1644	The serum levels of glycosylated fractions of clusterin, proline-arginine-rich end leucine-rich repeat protein, and haptoglobin in patients with ESCC were remarkably higher than those in healthy controls.
36157541	6	105	theme	procathepsin	1307:1318	arg1	D					1320:1320	total procathepsin D	1301:1320	total procathepsin D	1301:1320	Western blot analysis validated the up-regulation of total procathepsin D and high-mannose procathepsin D, and the down-regulation of total haptoglobin, high-mannose clusterin, and GlcNAc/sialic acid-containing fraction of 14-3-3ζ in ESCC tissues.
36157541	3	106	link	N-linked	465:472	arg1	glycoproteins					474:486	N-linked glycoproteins	465:486	N-linked glycoproteins	465:486	METHODS Using tandem multilectin affinity chromatography for enrichment of N-linked glycoproteins, we performed N-linked glycoproteomic profiling in ESCC tissues by two-dimensional gel electrophoresis (2-DE)-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel, followed by validation of candidate glycoprotein biomarkers by Western blot.
36297925	6	0	theme	polysaccharides	1215:1229	arg1	arrangement					1187:1197	the common supramolecular structural arrangement	1150:1197	the common supramolecular structural arrangement of proteins and polysaccharides	1150:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	7	1	theme	interchain	1559:1568	arg1	bonds					1579:1583	interchain physical bonds	1559:1583	interchain physical bonds	1559:1583	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	6	2	theme	conformational	1385:1398	arg1	rearrangements					1400:1413	the conformational rearrangements	1381:1413	the conformational rearrangements of the studied system	1381:1435	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	2	3	theme	hydrogels	303:311	arg1	morphology					289:298	the morphology	285:298	the morphology of hydrogels	285:311	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	2	3	theme	hydrogels	303:311	arg1	properties					334:343	their mechanical properties	317:343	their mechanical properties	317:343	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	6	4	theme	entanglements	1299:1311	arg1	network					1266:1272	network	1266:1272	network of macromolecular chains entanglements	1266:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	4	5	theme	components	705:714	arg1	structures					680:689	the morphological structures	662:689	the morphological structures of individual components	662:714	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	1	6	theme	κ-carrageenan-gelatin	179:199	arg1	system					201:206	a composite κ-carrageenan-gelatin system	167:206	a composite κ-carrageenan-gelatin system	167:206	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	6	7	theme	macromolecular	1277:1290	arg1	entanglements					1299:1311	macromolecular chains entanglements	1277:1311	macromolecular chains entanglements	1277:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	3	8	from	information	513:523	arg1	structure					532:540	structure	532:540	structure	532:540	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	3	8	from	information	513:523	arg1	performance					557:567	mechanical performance	546:567	mechanical performance	546:567	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	6	9	theme	network	1266:1272	arg1	density					1255:1261	the density	1251:1261	the density of network of macromolecular chains entanglements	1251:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	4	10	theme	morphological	666:678	arg1	structures					680:689	the morphological structures	662:689	the morphological structures of individual components	662:714	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	5	11	theme	chains	934:939	arg1	parking					915:921	the substantially denser parking	890:921	the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts	890:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	6	12	theme	Close	1046:1050	arg1	results					1052:1058	Close results	1046:1058	Close results	1046:1058	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	6	13	theme	energy	1344:1349	arg1	costs					1351:1355	the energy costs	1340:1355	the energy costs	1340:1355	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	5	14	from	parking	915:921	arg1	system					958:963	the composite system	944:963	the composite system due to a significant increase in intermolecular protein-polysaccharide contacts	944:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	1	15	theme	physicochemical	116:130	arg1	methods					132:138	complex physicochemical methods	108:138	complex physicochemical methods	108:138	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	6	16	theme	studied	1422:1428	arg1	system					1430:1435	the studied system	1418:1435	the studied system	1418:1435	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	5	17	theme	protein-polysaccharide	1013:1034	arg1	contacts					1036:1043	intermolecular protein-polysaccharide contacts	998:1043	intermolecular protein-polysaccharide contacts	998:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	7	18	dep	size	1536:1539	arg1	associate					1526:1534	associate	1526:1534	associate	1526:1534	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	4	19	theme	composite	741:749	arg1	hydrogels					774:782	the composite protein-polysaccharide hydrogels	737:782	the composite protein-polysaccharide hydrogels	737:782	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	5	20	theme	gelatin	834:840	arg1	testing					815:821	the mechanical testing	800:821	the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel	800:881	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	5	21	theme	significant	974:984	arg1	increase					986:993	a significant increase	972:993	a significant increase in intermolecular protein-polysaccharide contacts	972:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	6	22	theme	estimations-the	1099:1113	arg1	increase					1239:1246	the increase	1235:1246	the increase in the density of network of macromolecular chains entanglements	1235:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	6	22	theme	estimations-the	1099:1113	arg1	force					1123:1127	the SAXS estimations-the driving force	1090:1127	the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides	1090:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	8	23	theme	gel	1627:1629	arg1	plasticity					1631:1640	the composite gel plasticity	1613:1640	the composite gel plasticity	1613:1640	This led to an increase in the composite gel plasticity, whereas the enlargement of scattering particles made the novel gel system not only more rigid, but also more fragile.
36297925	6	24	from	increase	1328:1335	arg1	costs					1351:1355	the energy costs	1340:1355	the energy costs	1340:1355	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	8	25	from	increase	1601:1608	arg1	plasticity					1631:1640	the composite gel plasticity	1613:1640	the composite gel plasticity	1613:1640	This led to an increase in the composite gel plasticity, whereas the enlargement of scattering particles made the novel gel system not only more rigid, but also more fragile.
36297925	3	26	theme	hydrogel	594:601	arg1	structure					532:540	structure	532:540	structure	532:540	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	3	26	theme	hydrogel	594:601	arg1	performance					557:567	mechanical performance	546:567	mechanical performance	546:567	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	5	27	theme	mechanical	804:813	arg1	testing					815:821	the mechanical testing	800:821	the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel	800:881	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	7	28	theme	macromolecular	1459:1472	arg1	arrangement					1474:1484	the macromolecular arrangement	1455:1484	the macromolecular arrangement	1455:1484	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	0	29	theme	Supramolecular	0:13	arg1	Structure					15:23	Supramolecular Structure	0:23	Supramolecular Structure	0:23	Supramolecular Structure and Mechanical Performance of κ-Carrageenan-Gelatin Gel.
36297925	5	30	from	increase	986:993	arg1	contacts					1036:1043	intermolecular protein-polysaccharide contacts	998:1043	intermolecular protein-polysaccharide contacts	998:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	3	31	dep	structure	532:540	arg1	the					528:530	the	528:530	the	528:530	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	6	32	theme	structural	1176:1185	arg1	arrangement					1187:1197	the common supramolecular structural arrangement	1150:1197	the common supramolecular structural arrangement of proteins and polysaccharides	1150:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	5	33	theme	denser	908:913	arg1	parking					915:921	the substantially denser parking	890:921	the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts	890:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	6	34	theme	common	1154:1159	arg1	arrangement					1187:1197	the common supramolecular structural arrangement	1150:1197	the common supramolecular structural arrangement of proteins and polysaccharides	1150:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	8	35	theme	particles	1681:1689	arg1	enlargement					1655:1665	the enlargement	1651:1665	the enlargement of scattering particles	1651:1689	This led to an increase in the composite gel plasticity, whereas the enlargement of scattering particles made the novel gel system not only more rigid, but also more fragile.
36297925	5	36	theme	engineered	846:855	arg1	gel					879:881	engineered κ-carrageenan-gelatin gel	846:881	engineered κ-carrageenan-gelatin gel	846:881	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	2	37	from	characteristics	414:428	arg1	example					374:380	the example	370:380	the example of changes in their rheological characteristics	370:428	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	6	38	from	increase	1239:1246	arg1	density					1255:1261	the density	1251:1261	the density of network of macromolecular chains entanglements	1251:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	5	39	theme	gel	879:881	arg1	testing					815:821	the mechanical testing	800:821	the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel	800:881	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	8	40	theme	gel	1706:1708	arg1	system					1710:1715	the novel gel system	1696:1715	the novel gel system	1696:1715	This led to an increase in the composite gel plasticity, whereas the enlargement of scattering particles made the novel gel system not only more rigid, but also more fragile.
36297925	1	41	theme	initial	240:246	arg1	gel					256:258	initial protein gel	240:258	initial protein gel	240:258	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	7	42	from	increase	1443:1450	arg1	arrangement					1474:1484	the macromolecular arrangement	1455:1484	the macromolecular arrangement	1455:1484	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	4	43	theme	AFM	638:640	arg1	combination					608:618	The combination	604:618	The combination of PXRD, SAXS and AFM results	604:648	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	5	44	theme	κ-carrageenan-gelatin	857:877	arg1	gel					879:881	engineered κ-carrageenan-gelatin gel	846:881	engineered κ-carrageenan-gelatin gel	846:881	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	2	45	theme	changes	385:391	arg1	example					374:380	the example	370:380	the example of changes in their rheological characteristics	370:428	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	4	46	theme	PXRD	623:626	arg1	combination					608:618	The combination	604:618	The combination of PXRD, SAXS and AFM results	604:648	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	3	47	theme	κ-carrageenan-gelatin	572:592	arg1	hydrogel					594:601	κ-carrageenan-gelatin hydrogel	572:601	κ-carrageenan-gelatin hydrogel	572:601	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	2	48	from	example	374:380	arg1	characteristics					414:428	their rheological characteristics	396:428	their rheological characteristics	396:428	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	7	49	theme	physical	1570:1577	arg1	bonds					1579:1583	interchain physical bonds	1559:1583	interchain physical bonds	1559:1583	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	4	50	theme	SAXS	629:632	arg1	combination					608:618	The combination	604:618	The combination of PXRD, SAXS and AFM results	604:648	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	1	51	theme	system	201:206	arg1	features					155:162	the structural features	140:162	the structural features of a composite κ-carrageenan-gelatin system	140:206	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	1	52	theme	composite	169:177	arg1	system					201:206	a composite κ-carrageenan-gelatin system	167:206	a composite κ-carrageenan-gelatin system	167:206	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	0	53	theme	κ-Carrageenan-Gelatin	55:75	arg1	Gel					77:79	κ-Carrageenan-Gelatin Gel	55:79	κ-Carrageenan-Gelatin Gel	55:79	Supramolecular Structure and Mechanical Performance of κ-Carrageenan-Gelatin Gel.
36297925	7	54	theme	bonds	1579:1583	arg1	growth					1549:1554	the growth	1545:1554	the growth of interchain physical bonds	1545:1583	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	7	54	theme	bonds	1579:1583	arg1	growth					1497:1502	the growth	1493:1502	the growth of the supramolecular associate size	1493:1539	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	2	55	from	changes	385:391	arg1	characteristics					414:428	their rheological characteristics	396:428	their rheological characteristics	396:428	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	6	56	theme	chains	1292:1297	arg1	entanglements					1299:1311	macromolecular chains entanglements	1277:1311	macromolecular chains entanglements	1277:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	4	57	theme	individual	694:703	arg1	components					705:714	individual components	694:714	individual components	694:714	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	5	58	theme	composite	948:956	arg1	system					958:963	the composite system	944:963	the composite system due to a significant increase in intermolecular protein-polysaccharide contacts	944:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	2	59	theme	mechanical	323:332	arg1	properties					334:343	their mechanical properties	317:343	their mechanical properties	317:343	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	7	60	theme	size	1536:1539	arg1	growth					1549:1554	the growth	1545:1554	the growth of interchain physical bonds	1545:1583	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	7	60	theme	size	1536:1539	arg1	growth					1497:1502	the growth	1493:1502	the growth of the supramolecular associate size	1493:1539	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	1	61	theme	structural	144:153	arg1	features					155:162	the structural features	140:162	the structural features of a composite κ-carrageenan-gelatin system	140:206	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	5	62	theme	polymer	926:932	arg1	chains					934:939	polymer chains	926:939	polymer chains	926:939	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	7	63	theme	supramolecular	1511:1524	arg1	size					1536:1539	the supramolecular associate size	1507:1539	the supramolecular associate size	1507:1539	This increase in the macromolecular arrangement led to the growth of the supramolecular associate size and the growth of interchain physical bonds.
36297925	4	64	dep	PXRD	623:626	arg1	results					642:648	results	642:648	results	642:648	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	1	65	theme	complex	108:114	arg1	methods					132:138	complex physicochemical methods	108:138	complex physicochemical methods	108:138	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	4	66	theme	protein-polysaccharide	751:772	arg1	hydrogels					774:782	the composite protein-polysaccharide hydrogels	737:782	the composite protein-polysaccharide hydrogels	737:782	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	6	67	theme	system	1430:1435	arg1	rearrangements					1400:1413	the conformational rearrangements	1381:1413	the conformational rearrangements of the studied system	1381:1435	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	5	68	theme	intermolecular	998:1011	arg1	contacts					1036:1043	intermolecular protein-polysaccharide contacts	998:1043	intermolecular protein-polysaccharide contacts	998:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	5	69	theme	testing	815:821	arg1	results					789:795	The results	785:795	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel	785:881	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	5	70	theme	due	965:967	arg1	system					958:963	the composite system	944:963	the composite system due to a significant increase in intermolecular protein-polysaccharide contacts	944:1043	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	8	71	theme	composite	1617:1625	arg1	plasticity					1631:1640	the composite gel plasticity	1613:1640	the composite gel plasticity	1613:1640	This led to an increase in the composite gel plasticity, whereas the enlargement of scattering particles made the novel gel system not only more rigid, but also more fragile.
36297925	5	72	theme	initial	826:832	arg1	gelatin					834:840	initial gelatin	826:840	initial gelatin	826:840	The results of the mechanical testing of initial gelatin and engineered κ-carrageenan-gelatin gel showed the substantially denser parking of polymer chains in the composite system due to a significant increase in intermolecular protein-polysaccharide contacts.
36297925	6	73	theme	driving	1115:1121	arg1	increase					1239:1246	the increase	1235:1246	the increase in the density of network of macromolecular chains entanglements	1235:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	6	73	theme	driving	1115:1121	arg1	force					1123:1127	the SAXS estimations-the driving force	1090:1127	the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides	1090:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	6	74	theme	SAXS	1094:1097	arg1	increase					1239:1246	the increase	1235:1246	the increase in the density of network of macromolecular chains entanglements	1235:1311	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	6	74	theme	SAXS	1094:1097	arg1	force					1123:1127	the SAXS estimations-the driving force	1090:1127	the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides	1090:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	4	75	located	observed	725:732	arg1	hydrogels					774:782	the composite protein-polysaccharide hydrogels	737:782	the composite protein-polysaccharide hydrogels	737:782	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	4	75	located	observed	725:732	arg2	structures					680:689	the morphological structures	662:689	the morphological structures of individual components	662:714	The combination of PXRD, SAXS and AFM results showed that the morphological structures of individual components were not observed in the composite protein-polysaccharide hydrogels.
36297925	6	76	theme	proteins	1202:1209	arg1	arrangement					1187:1197	the common supramolecular structural arrangement	1150:1197	the common supramolecular structural arrangement of proteins and polysaccharides	1150:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	6	77	theme	arrangement	1187:1197	arg1	formation					1137:1145	the formation	1133:1145	the formation of the common supramolecular structural arrangement of proteins and polysaccharides	1133:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	8	78	theme	scattering	1670:1679	arg1	particles					1681:1689	scattering particles	1670:1689	scattering particles	1670:1689	This led to an increase in the composite gel plasticity, whereas the enlargement of scattering particles made the novel gel system not only more rigid, but also more fragile.
36297925	2	79	theme	rheological	402:412	arg1	characteristics					414:428	their rheological characteristics	396:428	their rheological characteristics	396:428	The correlation between the morphology of hydrogels and their mechanical properties was demonstrated through the example of changes in their rheological characteristics.
36297925	0	80	theme	Mechanical	29:38	arg1	Performance					40:50	Mechanical Performance	29:50	Mechanical Performance	29:50	Supramolecular Structure and Mechanical Performance of κ-Carrageenan-Gelatin Gel.
36297925	1	81	with	comparison	224:233	arg1	gel					256:258	initial protein gel	240:258	initial protein gel	240:258	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	6	82	theme	supramolecular	1161:1174	arg1	arrangement					1187:1197	the common supramolecular structural arrangement	1150:1197	the common supramolecular structural arrangement of proteins and polysaccharides	1150:1229	Close results were indirectly followed from the SAXS estimations-the driving force for the formation of the common supramolecular structural arrangement of proteins and polysaccharides was the increase in the density of network of macromolecular chains entanglements; therefore, an increase in the energy costs was necessary to change the conformational rearrangements of the studied system.
36297925	3	83	dep	PXRD	464:467	arg1	approaches					493:502	approaches	493:502	approaches	493:502	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	8	84	theme	novel	1700:1704	arg1	system					1710:1715	the novel gel system	1696:1715	the novel gel system	1696:1715	This led to an increase in the composite gel plasticity, whereas the enlargement of scattering particles made the novel gel system not only more rigid, but also more fragile.
36297925	0	85	theme	Gel	77:79	arg1	Structure					15:23	Supramolecular Structure	0:23	Supramolecular Structure	0:23	Supramolecular Structure and Mechanical Performance of κ-Carrageenan-Gelatin Gel.
36297925	0	85	theme	Gel	77:79	arg1	Performance					40:50	Mechanical Performance	29:50	Mechanical Performance	29:50	Supramolecular Structure and Mechanical Performance of κ-Carrageenan-Gelatin Gel.
36297925	3	86	theme	new	509:511	arg1	information					513:523	new information	509:523	new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel	509:601	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
36297925	1	87	theme	protein	248:254	arg1	gel					256:258	initial protein gel	240:258	initial protein gel	240:258	In this work, by means of complex physicochemical methods the structural features of a composite κ-carrageenan-gelatin system were studied in comparison with initial protein gel.
36297925	3	88	theme	mechanical	546:555	arg1	performance					557:567	mechanical performance	546:567	mechanical performance	546:567	The experiments carried out with PXRD, SAXS, AFM and rheology approaches gave new information on the structure and mechanical performance of κ-carrageenan-gelatin hydrogel.
37271761	4	0	theme	efficient	1049:1057	arg1	UIO-PBA&FDP					1025:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	0	theme	efficient	1049:1057	arg1	material					1065:1072	an efficient HILIC material	1046:1072	an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples	1046:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	2	1	dep	synthesized	488:498	arg1	characterized					501:513	characterized	501:513	characterized with the desirable excellent hydrophilicity	501:557	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	0	2	link	N-linked	112:119	arg1	glycopeptides					121:133	N-linked glycopeptides	112:133	N-linked glycopeptides	112:133	Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.
37271761	3	3	theme	N-linked	769:776	arg1	glycopeptides					778:790	359 N-linked glycopeptides	765:790	359 N-linked glycopeptides corresponding to 104 glycoproteins	765:825	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	4	4	link	N-linked	1096:1103	arg1	glycopeptides					1105:1117	N-linked glycopeptides	1096:1117	N-linked glycopeptides from complex biological samples	1096:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	0	5	theme	glycopeptides	121:133	arg1	enrichment					98:107	HILIC enrichment	92:107	HILIC enrichment of N-linked glycopeptides	92:133	Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.
37271761	1	6	gly	glycopeptides	212:224	arg2	glycopeptides					212:224	glycopeptides	212:224	glycopeptides in proteomics	212:238	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	2	7	theme	metal-organic	326:338	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	7	theme	metal-organic	326:338	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	7	theme	metal-organic	326:338	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	1	8	from	extraction	198:207	arg1	proteomics					229:238	proteomics	229:238	proteomics	229:238	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	3	9	theme	human	870:874	arg1	serum					876:880	digested human serum	861:880	digested human serum	861:880	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	0	10	theme	N-linked	112:119	arg1	glycopeptides					121:133	N-linked glycopeptides	112:133	N-linked glycopeptides	112:133	Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.
37271761	3	11	gly	glycoproteins	813:825	arg1	glycoproteins					813:825	104 glycoproteins	809:825	104 glycoproteins	809:825	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	2	12	theme	Zr-based	317:324	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	12	theme	Zr-based	317:324	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	12	theme	Zr-based	317:324	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	3	13	theme	serum	876:880	arg1	μL					855:856	only 1 μL	848:856	only 1 μL of digested human serum	848:880	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	1	14	theme	HILIC	140:144	arg1	strategy					181:188	a greatly compatible strategy	160:188	a greatly compatible strategy for the extraction of glycopeptides in proteomics	160:238	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	1	14	theme	HILIC	140:144	arg1	enrichment					146:155	The HILIC enrichment	136:155	The HILIC enrichment	136:155	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	2	15	theme	hydrophilic	692:702	arg1	UIO-PBA&FDP					704:714	the hydrophilic UIO-PBA&FDP	688:714	the hydrophilic UIO-PBA&FDP at a high level of ACN concentration	688:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	16	theme	dual-functionalized	297:315	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	16	theme	dual-functionalized	297:315	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	16	theme	dual-functionalized	297:315	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	4	17	theme	functionalized	1010:1023	arg1	UIO-PBA&FDP					1025:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	17	theme	functionalized	1010:1023	arg1	material					1065:1072	an efficient HILIC material	1046:1072	an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples	1046:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	2	18	theme	ACN	735:737	arg1	concentration					739:751	ACN concentration	735:751	ACN concentration	735:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	3	19	link	N-linked	769:776	arg1	glycopeptides					778:790	359 N-linked glycopeptides	765:790	359 N-linked glycopeptides corresponding to 104 glycoproteins	765:825	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	2	20	theme	excellent	534:542	arg1	hydrophilicity					544:557	the desirable excellent hydrophilicity	520:557	the desirable excellent hydrophilicity	520:557	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	4	21	theme	hydrophilic	998:1008	arg1	UIO-PBA&FDP					1025:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	21	theme	hydrophilic	998:1008	arg1	material					1065:1072	an efficient HILIC material	1046:1072	an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples	1046:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	3	22	theme	glycopeptides	778:790	arg1	total					756:760	A total	754:760	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins	754:825	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	0	23	theme	Boronic	0:6	arg1	acid					8:11	Boronic acid	0:11	Boronic acid	0:11	Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.
37271761	3	24	gly	glycopeptides	778:790	arg2	glycopeptides					778:790	359 N-linked glycopeptides	765:790	359 N-linked glycopeptides corresponding to 104 glycoproteins	765:825	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	3	25	theme	104	809:811	arg1	glycoproteins					813:825	104 glycoproteins	809:825	104 glycoproteins	809:825	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	2	26	theme	UIO-PBA&FDP	359:369	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	26	theme	UIO-PBA&FDP	359:369	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	26	theme	UIO-PBA&FDP	359:369	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	27	link	N-linked	603:610	arg1	glycopeptides					612:624	N-linked glycopeptides	603:624	N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration	603:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	28	gly	glycopeptides	670:682	arg2	glycopeptides					670:682	the glycopeptides	666:682	the glycopeptides	666:682	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	29	theme	phenylboronic	464:476	arg1	acid					478:481	carboxy phenylboronic acid)	456:482	carboxy phenylboronic acid)	456:482	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	4	30	from	samples	1143:1149	arg1	enrichment					1082:1091	the enrichment	1078:1091	the enrichment of N-linked glycopeptides from complex biological samples	1078:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	30	from	samples	1143:1149	arg1	glycopeptides					1105:1117	N-linked glycopeptides	1096:1117	N-linked glycopeptides from complex biological samples	1096:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	2	31	theme	high	721:724	arg1	level					726:730	a high level	719:730	a high level of ACN concentration	719:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	32	theme	material	350:357	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	32	theme	material	350:357	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	32	theme	material	350:357	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	0	33	gly	glycopeptides	121:133	arg2	glycopeptides					121:133	N-linked glycopeptides	112:133	N-linked glycopeptides	112:133	Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.
37271761	2	34	theme	framework	340:348	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	34	theme	framework	340:348	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	34	theme	framework	340:348	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	3	35	theme	identified	963:972	arg1	glycopeptides					974:986	the identified glycopeptides	959:986	the identified glycopeptides	959:986	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	4	36	theme	glycopeptides	1105:1117	arg1	enrichment					1082:1091	the enrichment	1078:1091	the enrichment of N-linked glycopeptides from complex biological samples	1078:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	37	from	enrichment	1082:1091	arg1	samples					1143:1149	complex biological samples	1124:1149	complex biological samples	1124:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	1	38	theme	glycopeptides	212:224	arg1	extraction					198:207	the extraction	194:207	the extraction of glycopeptides in proteomics	194:238	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	4	39	theme	HILIC	1059:1063	arg1	UIO-PBA&FDP					1025:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	39	theme	HILIC	1059:1063	arg1	material					1065:1072	an efficient HILIC material	1046:1072	an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples	1046:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	2	40	theme	boric	251:255	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	40	theme	boric	251:255	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	40	theme	boric	251:255	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	4	41	theme	N-linked	1096:1103	arg1	glycopeptides					1105:1117	N-linked glycopeptides	1096:1117	N-linked glycopeptides from complex biological samples	1096:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	2	42	gly	glycopeptides	612:624	arg2	glycopeptides					612:624	N-linked glycopeptides	603:624	N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration	603:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	0	43	theme	hydrophilic	69:79	arg1	Zr-MOF					81:86	highly hydrophilic Zr-MOF	62:86	highly hydrophilic Zr-MOF	62:86	Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.
37271761	2	44	theme	glycopeptides	612:624	arg1	enrichment					589:598	the enrichment	585:598	the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration	585:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	4	45	gly	glycopeptides	1105:1117	arg2	glycopeptides					1105:1117	N-linked glycopeptides	1096:1117	N-linked glycopeptides from complex biological samples	1096:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	45	gly	glycopeptides	1105:1117	arg1	samples					1143:1149	complex biological samples	1124:1149	complex biological samples	1124:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	3	46	gly	glycopeptides	974:986	arg2	glycopeptides					974:986	the identified glycopeptides	959:986	the identified glycopeptides	959:986	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	2	47	theme	HILIC	640:644	arg1	interaction					646:656	the HILIC interaction	636:656	the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration	636:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	3	48	theme	UIO-PBA&FDP	903:913	arg1	enrichment					889:898	the enrichment	885:898	the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides	885:986	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	2	49	theme	N-linked	603:610	arg1	glycopeptides					612:624	N-linked glycopeptides	603:624	N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration	603:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	3	50	theme	high	942:945	arg1	coverage					947:954	a superiorly high coverage	929:954	a superiorly high coverage of the identified glycopeptides	929:986	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	2	51	theme	FDP	292:294	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	51	theme	FDP	292:294	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	51	theme	FDP	292:294	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	1	52	from	proteomics	229:238	arg1	extraction					198:207	the extraction	194:207	the extraction of glycopeptides in proteomics	194:238	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	2	53	theme	concentration	739:751	arg1	level					726:730	a high level	719:730	a high level of ACN concentration	719:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	3	54	theme	glycopeptides	974:986	arg1	coverage					947:954	a superiorly high coverage	929:954	a superiorly high coverage of the identified glycopeptides	929:986	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	3	55	theme	digested	861:868	arg1	serum					876:880	digested human serum	861:880	digested human serum	861:880	A total of 359 N-linked glycopeptides corresponding to 104 glycoproteins were identified from only 1 μL of digested human serum by the enrichment of UIO-PBA&FDP, which showed a superiorly high coverage of the identified glycopeptides.
37271761	0	56	theme	HILIC	92:96	arg1	enrichment					98:107	HILIC enrichment	92:107	HILIC enrichment of N-linked glycopeptides	92:133	Boronic acid and fructose-1,6-diphosphate dual-functionalized highly hydrophilic Zr-MOF for HILIC enrichment of N-linked glycopeptides.
37271761	4	57	theme	biological	1132:1141	arg1	samples					1143:1149	complex biological samples	1124:1149	complex biological samples	1124:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	2	58	theme	fructose-1,6-diphosphate	266:289	arg1	UIO					372:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO	266:374	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	58	theme	fructose-1,6-diphosphate	266:289	arg1	abbreviation					383:394	the abbreviation	379:394	the abbreviation for the University of Oslo	379:421	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	58	theme	fructose-1,6-diphosphate	266:289	arg1	acid					257:260	a boric acid	249:260	a boric acid	249:260	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	1	59	from	glycopeptides	212:224	arg1	proteomics					229:238	proteomics	229:238	proteomics	229:238	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	2	60	from	level	726:730	arg1	glycopeptides					670:682	the glycopeptides	666:682	the glycopeptides	666:682	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	60	from	level	726:730	arg1	UIO-PBA&FDP					704:714	the hydrophilic UIO-PBA&FDP	688:714	the hydrophilic UIO-PBA&FDP at a high level of ACN concentration	688:751	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	61	theme	desirable	524:532	arg1	hydrophilicity					544:557	the desirable excellent hydrophilicity	520:557	the desirable excellent hydrophilicity	520:557	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	2	62	theme	carboxy	456:462	arg1	acid					478:481	carboxy phenylboronic acid)	456:482	carboxy phenylboronic acid)	456:482	Herein, a boric acid and fructose-1,6-diphosphate (FDP) dual-functionalized Zr-based metal-organic framework material UIO-PBA&FDP (UIO is the abbreviation for the University of Oslo, and PBA is the abbreviation for carboxy phenylboronic acid) was synthesized, characterized with the desirable excellent hydrophilicity and thus was explored for the enrichment of N-linked glycopeptides utilizing the HILIC interaction between the glycopeptides and the hydrophilic UIO-PBA&FDP at a high level of ACN concentration.
37271761	1	63	theme	compatible	170:179	arg1	strategy					181:188	a greatly compatible strategy	160:188	a greatly compatible strategy for the extraction of glycopeptides in proteomics	160:238	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	1	63	theme	compatible	170:179	arg1	enrichment					146:155	The HILIC enrichment	136:155	The HILIC enrichment	136:155	The HILIC enrichment is a greatly compatible strategy for the extraction of glycopeptides in proteomics.
37271761	4	64	theme	complex	1124:1130	arg1	samples					1143:1149	complex biological samples	1124:1149	complex biological samples	1124:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	65	theme	dual	993:996	arg1	UIO-PBA&FDP					1025:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP	989:1035	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
37271761	4	65	theme	dual	993:996	arg1	material					1065:1072	an efficient HILIC material	1046:1072	an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples	1046:1149	The dual hydrophilic functionalized UIO-PBA&FDP could be an efficient HILIC material for the enrichment of N-linked glycopeptides from complex biological samples.
36139350	0	0	theme	STT3A	93:97	arg1	Specificity					78:88	the Protein N-Linked Glycosylation Specificity	43:88	the Protein N-Linked Glycosylation Specificity of STT3A and STT3B	43:107	Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
36139350	4	1	gly	glycoproteins	762:774	arg1	glycoproteins					762:774	glycoproteins	762:774	glycoproteins	762:774	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	3	2	gly	glycopeptides	548:560	arg2	glycopeptides					548:560	4265 unique N-linked intact glycopeptides	520:560	4265 unique N-linked intact glycopeptides	520:560	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	7	3	theme	unfolded	1231:1238	arg1	response					1248:1255	the unfolded protein response	1227:1255	the unfolded protein response	1227:1255	Furthermore, the increased expression of the ATF6 and PERK indicated that the unfolded protein response also happened in STT3A-KO cells.
36139350	6	4	theme	KO	1029:1030	arg1	cells					1032:1036	KO cells	1029:1036	KO cells	1029:1036	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	7	5	theme	PERK	1207:1210	arg1	expression					1180:1189	the increased expression	1166:1189	the increased expression of the ATF6 and PERK	1166:1210	Furthermore, the increased expression of the ATF6 and PERK indicated that the unfolded protein response also happened in STT3A-KO cells.
36139350	2	6	theme	STT3A	372:376	arg1	specificity					357:367	the specificity	353:367	the specificity of STT3A and STT3B	353:386	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	8	7	theme	OST	1363:1365	arg1	subunit					1367:1373	the OST subunit	1359:1373	the OST subunit	1359:1373	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	8	8	gly	glycosylation	1408:1420	arg1	protein					1429:1435	the protein	1425:1435	the protein	1425:1435	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	0	9	theme	STT3B	103:107	arg1	Specificity					78:88	the Protein N-Linked Glycosylation Specificity	43:88	the Protein N-Linked Glycosylation Specificity of STT3A and STT3B	43:107	Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
36139350	3	10	theme	N-linked	532:539	arg1	glycopeptides					548:560	4265 unique N-linked intact glycopeptides	520:560	4265 unique N-linked intact glycopeptides	520:560	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	4	11	theme	STT3B	741:745	arg1	deletion					729:736	deletion	729:736	deletion of STT3B	729:745	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	4	12	theme	greater	683:689	arg1	impact					691:696	a greater impact	681:696	a greater impact	681:696	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	7	13	theme	protein	1240:1246	arg1	response					1248:1255	the unfolded protein response	1227:1255	the unfolded protein response	1227:1255	Furthermore, the increased expression of the ATF6 and PERK indicated that the unfolded protein response also happened in STT3A-KO cells.
36139350	8	14	theme	STT3B	1328:1332	arg1	specificity					1303:1313	the specificity	1299:1313	the specificity of STT3A and STT3B	1299:1332	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	1	15	theme	cotranslational	261:275	arg1	glycosylation					311:323	cotranslational and post-translocational N-linked glycosylation	261:323	cotranslational and post-translocational N-linked glycosylation	261:323	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	3	16	link	N-linked	532:539	arg1	glycopeptides					548:560	4265 unique N-linked intact glycopeptides	520:560	4265 unique N-linked intact glycopeptides	520:560	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	4	17	theme	protein	705:711	arg1	expression					713:722	the protein expression	701:722	the protein expression	701:722	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	5	18	gly	fucosylated	836:846	arg1	N-glycans					848:856	fucosylated N-glycans	836:856	fucosylated N-glycans	836:856	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	5	19	theme	fucosylated	836:846	arg1	N-glycans					848:856	fucosylated N-glycans	836:856	fucosylated N-glycans	836:856	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	5	20	theme	STT3A-KO	876:883	arg1	cells					885:889	STT3A-KO cells	876:889	STT3A-KO cells	876:889	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	4	21	theme	STT3A	666:670	arg1	gene					672:675	the STT3A gene	662:675	the STT3A gene	662:675	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	5	22	theme	total	790:794	arg1	N-glycans					809:817	total mannosylated N-glycans	790:817	total mannosylated N-glycans	790:817	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	6	23	theme	ENPL	1069:1072	arg1	hyperglycosylation					1047:1064	the hyperglycosylation	1043:1064	the hyperglycosylation of ENPL	1043:1072	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	6	24	gly	hyperglycosylated	983:999	arg1	proteins					1001:1008	hyperglycosylated proteins	983:1008	hyperglycosylated proteins	983:1008	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	3	25	theme	unique	525:530	arg1	glycopeptides					548:560	4265 unique N-linked intact glycopeptides	520:560	4265 unique N-linked intact glycopeptides	520:560	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	6	26	theme	reticulum	1104:1112	arg1	stress					1119:1124	the endoplasmic reticulum (ER) stress	1088:1124	the endoplasmic reticulum (ER) stress due to the STT3A deletion	1088:1150	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	1	27	theme	oligosaccharyltransferase	165:189	arg1	complex					191:197	the oligosaccharyltransferase complex	161:197	the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells)	161:234	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	5	28	theme	mannosylated	796:807	arg1	N-glycans					809:817	total mannosylated N-glycans	790:817	total mannosylated N-glycans	790:817	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	6	29	theme	endoplasmic	1092:1102	arg1	ER					1115:1116	ER	1115:1116	ER	1115:1116	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	6	29	theme	endoplasmic	1092:1102	arg1	reticulum					1104:1112	endoplasmic reticulum	1092:1112	the endoplasmic reticulum (ER) stress due to the STT3A deletion	1088:1150	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	3	30	theme	629	566:568	arg1	glycosites					570:579	629 glycosites	566:579	629 glycosites	566:579	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	7	31	theme	increased	1170:1178	arg1	expression					1180:1189	the increased expression	1166:1189	the increased expression of the ATF6 and PERK	1166:1210	Furthermore, the increased expression of the ATF6 and PERK indicated that the unfolded protein response also happened in STT3A-KO cells.
36139350	1	32	theme	complex	191:197	arg1	subunits					149:156	the main catalytic subunits	130:156	the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively	130:337	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	1	32	theme	complex	191:197	arg1	STT3B					120:124	STT3B	120:124	STT3B	120:124	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	1	32	theme	complex	191:197	arg1	STT3A					110:114	STT3A	110:114	STT3A	110:114	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	0	33	theme	Proteome	0:7	arg1	Analyses					27:34	Proteome and Glycoproteome Analyses	0:34	Analyses	27:34	Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
36139350	5	34	theme	glycan-related	944:957	arg1	enzymes					959:965	glycan-related enzymes	944:965	glycan-related enzymes	944:965	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	4	35	theme	gene	672:675	arg1	Deletion					650:657	Deletion	650:657	Deletion of the STT3A gene	650:675	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	1	36	theme	post-translocational	281:300	arg1	glycosylation					311:323	cotranslational and post-translocational N-linked glycosylation	261:323	cotranslational and post-translocational N-linked glycosylation	261:323	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	5	37	theme	enzymes	959:965	arg1	expression					930:939	the differential expression	913:939	the differential expression of glycan-related enzymes	913:965	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	2	38	theme	gene	447:450	arg1	KO					463:464	KO	463:464	KO	463:464	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	2	38	theme	gene	447:450	arg1	knock-out					452:460	the gene knock-out	443:460	the gene knock-out (KO)	443:465	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	1	39	theme	N-linked	302:309	arg1	glycosylation					311:323	cotranslational and post-translocational N-linked glycosylation	261:323	cotranslational and post-translocational N-linked glycosylation	261:323	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	3	40	theme	3961	505:508	arg1	proteins					510:517	3961 proteins	505:517	3961 proteins	505:517	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	8	41	link	N-linked	1399:1406	arg1	glycosylation					1408:1420	N-linked glycosylation	1399:1420	N-linked glycosylation of the protein	1399:1435	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	3	42	gly	glycoproteins	598:610	arg1	glycoproteins					598:610	349 glycoproteins	594:610	349 glycoproteins	594:610	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	2	43	theme	HEK293	481:486	arg1	cells					488:492	wild-type HEK293 cells	471:492	wild-type HEK293 cells	471:492	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	8	44	theme	STT3A	1318:1322	arg1	specificity					1303:1313	the specificity	1299:1313	the specificity of STT3A and STT3B	1299:1332	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	6	45	gly	hyperglycosylation	1047:1064	arg1	ENPL					1069:1072	ENPL	1069:1072	ENPL	1069:1072	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	8	46	theme	protein	1429:1435	arg1	glycosylation					1408:1420	N-linked glycosylation	1399:1420	N-linked glycosylation of the protein	1399:1435	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	1	47	dep	complex	191:197	arg1	OST-B					210:214	OST-B	210:214	OST-B	210:214	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	1	47	dep	complex	191:197	arg1	OST-A					200:204	OST-A	200:204	OST-A	200:204	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	2	48	theme	wild-type	471:479	arg1	cells					488:492	wild-type HEK293 cells	471:492	wild-type HEK293 cells	471:492	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	5	49	gly	mannosylated	796:807	arg1	N-glycans					809:817	total mannosylated N-glycans	790:817	total mannosylated N-glycans	790:817	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	1	50	from	OST-B	210:214	arg1	cells					229:233	mammalian cells	219:233	mammalian cells	219:233	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	2	51	theme	glycoproteomic	416:429	arg1	analyses					431:438	proteomic and glycoproteomic analyses	402:438	proteomic and glycoproteomic analyses	402:438	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	0	52	theme	N-Linked	55:62	arg1	Glycosylation					64:76	Protein N-Linked Glycosylation	47:76	the Protein N-Linked Glycosylation Specificity of STT3A and STT3B	43:107	Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
36139350	7	53	theme	ATF6	1198:1201	arg1	expression					1180:1189	the increased expression	1166:1189	the increased expression of the ATF6 and PERK	1166:1210	Furthermore, the increased expression of the ATF6 and PERK indicated that the unfolded protein response also happened in STT3A-KO cells.
36139350	6	54	theme	hyperglycosylated	983:999	arg1	proteins					1001:1008	hyperglycosylated proteins	983:1008	hyperglycosylated proteins	983:1008	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	6	55	theme	due	1126:1128	arg1	stress					1119:1124	the endoplasmic reticulum (ER) stress	1088:1124	the endoplasmic reticulum (ER) stress due to the STT3A deletion	1088:1150	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	3	56	gly	glycosites	570:579	arg2	glycosites					570:579	629 glycosites	566:579	629 glycosites	566:579	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	0	57	theme	Protein	47:53	arg1	Glycosylation					64:76	Protein N-Linked Glycosylation	47:76	the Protein N-Linked Glycosylation Specificity of STT3A and STT3B	43:107	Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
36139350	8	58	theme	protein	1453:1459	arg1	expression					1461:1470	protein expression	1453:1470	protein expression	1453:1470	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	1	59	from	OST-A	200:204	arg1	cells					229:233	mammalian cells	219:233	mammalian cells	219:233	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	8	60	from	defects	1348:1354	arg1	subunit					1367:1373	the OST subunit	1359:1373	the OST subunit	1359:1373	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	7	61	dep	ATF6	1198:1201	arg1	the					1194:1196	the	1194:1196	the	1194:1196	Furthermore, the increased expression of the ATF6 and PERK indicated that the unfolded protein response also happened in STT3A-KO cells.
36139350	4	62	contain	had	677:679	arg1	Deletion					650:657	Deletion	650:657	Deletion of the STT3A gene	650:675	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	4	62	contain	had	677:679	arg2	impact					691:696	a greater impact	681:696	a greater impact	681:696	Deletion of the STT3A gene had a greater impact on the protein expression than deletion of STT3B, especially on glycoproteins.
36139350	3	63	dep	N-linked	532:539	arg1	intact					541:546	intact	541:546	intact	541:546	In total, 3961 proteins, 4265 unique N-linked intact glycopeptides and 629 glycosites representing 349 glycoproteins were identified from all these cells.
36139350	1	64	theme	mammalian	219:227	arg1	cells					229:233	mammalian cells	219:233	mammalian cells	219:233	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	7	65	theme	STT3A-KO	1274:1281	arg1	cells					1283:1287	STT3A-KO cells	1274:1287	STT3A-KO cells	1274:1287	Furthermore, the increased expression of the ATF6 and PERK indicated that the unfolded protein response also happened in STT3A-KO cells.
36139350	8	66	theme	N-linked	1399:1406	arg1	glycosylation					1408:1420	N-linked glycosylation	1399:1420	N-linked glycosylation of the protein	1399:1435	Overall, the specificity of STT3A and STT3B revealed that defects in the OST subunit not only broadly affect N-linked glycosylation of the protein but also affect protein expression.
36139350	1	67	link	N-linked	302:309	arg1	glycosylation					311:323	cotranslational and post-translocational N-linked glycosylation	261:323	cotranslational and post-translocational N-linked glycosylation	261:323	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	2	68	theme	STT3B	382:386	arg1	specificity					357:367	the specificity	353:367	the specificity of STT3A and STT3B	353:386	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	5	69	theme	differential	917:928	arg1	expression					930:939	the differential expression	913:939	the differential expression of glycan-related enzymes	913:965	In addition, total mannosylated N-glycans were reduced and fucosylated N-glycans were increased in STT3A-KO cells, which were caused by the differential expression of glycan-related enzymes.
36139350	1	70	theme	main	134:137	arg1	subunits					149:156	the main catalytic subunits	130:156	the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively	130:337	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	1	70	theme	main	134:137	arg1	STT3B					120:124	STT3B	120:124	STT3B	120:124	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	1	70	theme	main	134:137	arg1	STT3A					110:114	STT3A	110:114	STT3A	110:114	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	2	71	theme	proteomic	402:410	arg1	analyses					431:438	proteomic and glycoproteomic analyses	402:438	proteomic and glycoproteomic analyses	402:438	To determine the specificity of STT3A and STT3B, we performed proteomic and glycoproteomic analyses in the gene knock-out (KO) and wild-type HEK293 cells.
36139350	0	72	theme	Glycosylation	64:76	arg1	Specificity					78:88	the Protein N-Linked Glycosylation Specificity	43:88	the Protein N-Linked Glycosylation Specificity of STT3A and STT3B	43:107	Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
36139350	6	73	theme	STT3A	1137:1141	arg1	deletion					1143:1150	the STT3A deletion	1133:1150	the STT3A deletion	1133:1150	Interestingly, hyperglycosylated proteins were identified in KO cells, and the hyperglycosylation of ENPL was caused by the endoplasmic reticulum (ER) stress due to the STT3A deletion.
36139350	0	74	theme	Glycoproteome	13:25	arg1	Analyses					27:34	Proteome and Glycoproteome Analyses	0:34	Analyses	27:34	Proteome and Glycoproteome Analyses Reveal the Protein N-Linked Glycosylation Specificity of STT3A and STT3B.
36139350	1	75	theme	catalytic	139:147	arg1	subunits					149:156	the main catalytic subunits	130:156	the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively	130:337	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	1	75	theme	catalytic	139:147	arg1	STT3B					120:124	STT3B	120:124	STT3B	120:124	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
36139350	1	75	theme	catalytic	139:147	arg1	STT3A					110:114	STT3A	110:114	STT3A	110:114	STT3A and STT3B are the main catalytic subunits of the oligosaccharyltransferase complex (OST-A and OST-B in mammalian cells), which primarily mediate cotranslational and post-translocational N-linked glycosylation, respectively.
37050384	3	0	theme	lower	622:626	arg1	g/mol					656:660	17.54 × 106 g/mol	644:660	17.54 × 106 g/mol	644:660	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	0	theme	lower	622:626	arg1	mass					638:641	a lower molecular mass	620:641	a lower molecular mass (17.54 × 106 g/mol)	620:661	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	7	1	theme	L-TPS-stabilized	995:1010	arg1	stable					1030:1035	stable	1030:1035	stable	1030:1035	Commercial TPS emulsions were most stable at pH 6, while the L-TPS-stabilized emulsion was most stable at pH 2.
37050384	7	1	theme	L-TPS-stabilized	995:1010	arg1	emulsion					1012:1019	the L-TPS-stabilized emulsion	991:1019	the L-TPS-stabilized emulsion	991:1019	Commercial TPS emulsions were most stable at pH 6, while the L-TPS-stabilized emulsion was most stable at pH 2.
37050384	8	2	from	effects	1130:1136	arg1	characteristics					1156:1170	emulsion characteristics	1147:1170	emulsion characteristics	1147:1170	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	5	3	theme	commercial	794:803	arg1	emulsions					820:828	commercial TPS-stabilized emulsions	794:828	commercial TPS-stabilized emulsions	794:828	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	2	4	theme	particle	445:452	arg1	size					454:457	particle size	445:457	particle size	445:457	The effects of pH on the properties of TPS emulsions were investigated by analyzing their zeta potential, particle size, apparent viscosity, and stability.
37050384	5	5	theme	TPS-stabilized	805:818	arg1	emulsions					820:828	commercial TPS-stabilized emulsions	794:828	commercial TPS-stabilized emulsions	794:828	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	6	6	theme	apparent	889:896	arg1	viscosity					898:906	the apparent viscosity	885:906	the apparent viscosity	885:906	At pH 2, the apparent viscosity was the lowest for L-TPS.
37050384	6	6	theme	apparent	889:896	arg1	lowest					916:921	lowest	916:921	lowest	916:921	At pH 2, the apparent viscosity was the lowest for L-TPS.
37050384	1	7	theme	commercial	323:332	arg1	TPS					334:336	three commercial TPS	317:336	three commercial TPS	317:336	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	4	8	theme	poor	725:728	arg1	stability					739:747	poor emulsion stability	725:747	poor emulsion stability	725:747	Thus, L-TPS exhibited the best emulsifying activity but gave poor emulsion stability.
37050384	0	9	theme	Different	91:99	arg1	pHs					101:103	Different pHs	91:103	Different pHs	91:103	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	7	10	from	pH	1040:1041	arg1	stable					1030:1035	stable	1030:1035	stable	1030:1035	Commercial TPS emulsions were most stable at pH 6, while the L-TPS-stabilized emulsion was most stable at pH 2.
37050384	7	10	from	pH	1040:1041	arg1	emulsion					1012:1019	the L-TPS-stabilized emulsion	991:1019	the L-TPS-stabilized emulsion	991:1019	Commercial TPS emulsions were most stable at pH 6, while the L-TPS-stabilized emulsion was most stable at pH 2.
37050384	8	11	theme	molecular	1205:1213	arg1	mass					1215:1218	the molecular mass	1201:1218	the molecular mass	1201:1218	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	3	12	theme	17.54	644:648	arg1	g/mol					656:660	17.54 × 106 g/mol	644:660	17.54 × 106 g/mol	644:660	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	12	theme	17.54	644:648	arg1	mass					638:641	a lower molecular mass	620:641	a lower molecular mass (17.54 × 106 g/mol)	620:661	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	2	13	theme	TPS	378:380	arg1	emulsions					382:390	TPS emulsions	378:390	TPS emulsions	378:390	The effects of pH on the properties of TPS emulsions were investigated by analyzing their zeta potential, particle size, apparent viscosity, and stability.
37050384	8	14	theme	macromolecular	1221:1234	arg1	characteristics					1236:1250	macromolecular characteristics	1221:1250	macromolecular characteristics	1221:1250	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	1	15	theme	types	186:190	arg1	composition					119:129	The chemical composition	106:129	The chemical composition	106:129	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	1	15	theme	types	186:190	arg1	characteristics					147:161	macromolecular characteristics	132:161	macromolecular characteristics	132:161	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	1	15	theme	types	186:190	arg1	structure					168:176	structure	168:176	structure	168:176	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	5	16	theme	droplet	754:760	arg1	sizes					762:766	droplet sizes	754:766	droplet sizes	754:766	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	3	17	theme	molecular	628:636	arg1	g/mol					656:660	17.54 × 106 g/mol	644:660	17.54 × 106 g/mol	644:660	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	17	theme	molecular	628:636	arg1	mass					638:641	a lower molecular mass	620:641	a lower molecular mass (17.54 × 106 g/mol)	620:661	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	4	18	theme	emulsion	730:737	arg1	stability					739:747	poor emulsion stability	725:747	poor emulsion stability	725:747	Thus, L-TPS exhibited the best emulsifying activity but gave poor emulsion stability.
37050384	8	19	theme	obtained	1050:1057	arg1	results					1059:1065	The obtained results	1046:1065	The obtained results	1046:1065	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	5	20	theme	emulsions	820:828	arg1	sizes					762:766	droplet sizes	754:766	droplet sizes	754:766	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	5	20	theme	emulsions	820:828	arg1	viscosity					781:789	apparent viscosity	772:789	apparent viscosity	772:789	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	0	21	theme	fuciform	9:16	arg1	Polysaccharides					18:32	Tremella fuciform Polysaccharides	0:32	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.	0:104	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	21	theme	fuciform	9:16	arg1	Physicochemical					47:61	Physicochemical	47:61	Physicochemical	47:61	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	21	theme	fuciform	9:16	arg1	Emulsion					68:75	Emulsion	68:75	Emulsion	68:75	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	21	theme	fuciform	9:16	arg1	Extraction					35:44	Extraction	35:44	Extraction	35:44	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	8	22	from	properties	1097:1106	arg1	characteristics					1156:1170	emulsion characteristics	1147:1170	emulsion characteristics	1147:1170	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	2	23	theme	pH	354:355	arg1	effects					343:349	The effects	339:349	The effects of pH on the properties of TPS emulsions	339:390	The effects of pH on the properties of TPS emulsions were investigated by analyzing their zeta potential, particle size, apparent viscosity, and stability.
37050384	0	24	theme	Tremella	0:7	arg1	Polysaccharides					18:32	Tremella fuciform Polysaccharides	0:32	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.	0:104	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	24	theme	Tremella	0:7	arg1	Physicochemical					47:61	Physicochemical	47:61	Physicochemical	47:61	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	24	theme	Tremella	0:7	arg1	Emulsion					68:75	Emulsion	68:75	Emulsion	68:75	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	24	theme	Tremella	0:7	arg1	Extraction					35:44	Extraction	35:44	Extraction	35:44	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	2	25	from	effects	343:349	arg1	properties					364:373	the properties	360:373	the properties of TPS emulsions	360:390	The effects of pH on the properties of TPS emulsions were investigated by analyzing their zeta potential, particle size, apparent viscosity, and stability.
37050384	5	26	theme	apparent	772:779	arg1	viscosity					781:789	apparent viscosity	772:789	apparent viscosity	772:789	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	4	27	theme	emulsifying	695:705	arg1	activity					707:714	the best emulsifying activity	686:714	the best emulsifying activity	686:714	Thus, L-TPS exhibited the best emulsifying activity but gave poor emulsion stability.
37050384	0	28	dep	Extraction	35:44	arg1	Properties					77:86	Properties	77:86	Properties	77:86	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	5	29	theme	acidic	855:860	arg1	environments					862:873	acidic environments	855:873	acidic environments	855:873	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	2	30	theme	emulsions	382:390	arg1	properties					364:373	the properties	360:373	the properties of TPS emulsions	360:390	The effects of pH on the properties of TPS emulsions were investigated by analyzing their zeta potential, particle size, apparent viscosity, and stability.
37050384	3	31	theme	×	650:650	arg1	g/mol					656:660	17.54 × 106 g/mol	644:660	17.54 × 106 g/mol	644:660	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	31	theme	×	650:650	arg1	mass					638:641	a lower molecular mass	620:641	a lower molecular mass (17.54 × 106 g/mol)	620:661	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	32	theme	commercial	587:596	arg1	TPS					598:600	commercial TPS	587:600	commercial TPS (0.73-0.87%)	587:613	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	32	theme	commercial	587:596	arg1	%					612:612	0.73-0.87%	603:612	0.73-0.87%	603:612	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	1	33	theme	fuciform	204:211	arg1	TPS					230:232	TPS	230:232	TPS	230:232	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	1	33	theme	fuciform	204:211	arg1	polysaccharides					213:227	Tremella fuciform polysaccharides	195:227	Tremella fuciform polysaccharides (TPS)	195:233	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	8	34	theme	emulsifying	1085:1095	arg1	properties					1097:1106	the emulsifying properties	1081:1106	the emulsifying properties of TPS varied	1081:1120	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	9	35	theme	food	1340:1343	arg1	application					1310:1320	the application	1306:1320	the application of TPS	1306:1327	This research is useful for expanding the application of TPS as a novel food ingredient in emulsions.
37050384	9	35	theme	food	1340:1343	arg1	ingredient					1345:1354	a novel food ingredient	1332:1354	a novel food ingredient in emulsions	1332:1367	This research is useful for expanding the application of TPS as a novel food ingredient in emulsions.
37050384	1	36	theme	polysaccharides	213:227	arg1	types					186:190	four types	181:190	four types of Tremella fuciform polysaccharides (TPS)	181:233	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	8	37	theme	pH	1141:1142	arg1	properties					1097:1106	the emulsifying properties	1081:1106	the emulsifying properties of TPS varied	1081:1120	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	8	37	theme	pH	1141:1142	arg1	effects					1130:1136	the effects	1126:1136	the effects of pH on emulsion characteristics	1126:1170	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	2	38	theme	zeta	429:432	arg1	potential					434:442	zeta potential	429:442	zeta potential	429:442	The effects of pH on the properties of TPS emulsions were investigated by analyzing their zeta potential, particle size, apparent viscosity, and stability.
37050384	7	39	theme	Commercial	934:943	arg1	emulsions					949:957	Commercial TPS emulsions	934:957	Commercial TPS emulsions	934:957	Commercial TPS emulsions were most stable at pH 6, while the L-TPS-stabilized emulsion was most stable at pH 2.
37050384	7	40	from	pH	979:980	arg1	stable					969:974	stable	969:974	stable	969:974	Commercial TPS emulsions were most stable at pH 6, while the L-TPS-stabilized emulsion was most stable at pH 2.
37050384	3	41	theme	higher	537:542	arg1	content					555:561	a higher percentage content	535:561	a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%)	535:613	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	9	42	from	ingredient	1345:1354	arg1	emulsions					1359:1367	emulsions	1359:1367	emulsions	1359:1367	This research is useful for expanding the application of TPS as a novel food ingredient in emulsions.
37050384	1	43	theme	chemical	110:117	arg1	composition					119:129	The chemical composition	106:129	The chemical composition	106:129	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	7	44	theme	TPS	945:947	arg1	emulsions					949:957	Commercial TPS emulsions	934:957	Commercial TPS emulsions	934:957	Commercial TPS emulsions were most stable at pH 6, while the L-TPS-stabilized emulsion was most stable at pH 2.
37050384	3	45	theme	percentage	544:553	arg1	content					555:561	a higher percentage content	535:561	a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%)	535:613	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	9	46	theme	novel	1334:1338	arg1	application					1310:1320	the application	1306:1320	the application of TPS	1306:1327	This research is useful for expanding the application of TPS as a novel food ingredient in emulsions.
37050384	9	46	theme	novel	1334:1338	arg1	ingredient					1345:1354	a novel food ingredient	1332:1354	a novel food ingredient in emulsions	1332:1367	This research is useful for expanding the application of TPS as a novel food ingredient in emulsions.
37050384	8	47	theme	emulsion	1147:1154	arg1	characteristics					1156:1170	emulsion characteristics	1147:1170	emulsion characteristics	1147:1170	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	0	48	from	pHs	101:103	arg1	Polysaccharides					18:32	Tremella fuciform Polysaccharides	0:32	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.	0:104	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	48	from	pHs	101:103	arg1	Physicochemical					47:61	Physicochemical	47:61	Physicochemical	47:61	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	48	from	pHs	101:103	arg1	Emulsion					68:75	Emulsion	68:75	Emulsion	68:75	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	48	from	pHs	101:103	arg1	Extraction					35:44	Extraction	35:44	Extraction	35:44	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	49	dep	Polysaccharides	18:32	arg1	Polysaccharides					18:32	Tremella fuciform Polysaccharides	0:32	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.	0:104	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	49	dep	Polysaccharides	18:32	arg1	Physicochemical					47:61	Physicochemical	47:61	Physicochemical	47:61	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	49	dep	Polysaccharides	18:32	arg1	Emulsion					68:75	Emulsion	68:75	Emulsion	68:75	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	0	49	dep	Polysaccharides	18:32	arg1	Extraction					35:44	Extraction	35:44	Extraction	35:44	Tremella fuciform Polysaccharides: Extraction, Physicochemical, and Emulsion Properties at Different pHs.
37050384	1	50	theme	macromolecular	132:145	arg1	characteristics					147:161	macromolecular characteristics	132:161	macromolecular characteristics	132:161	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	2	51	theme	apparent	460:467	arg1	viscosity					469:477	apparent viscosity	460:477	apparent viscosity	460:477	The effects of pH on the properties of TPS emulsions were investigated by analyzing their zeta potential, particle size, apparent viscosity, and stability.
37050384	3	52	theme	protein	566:572	arg1	content					555:561	a higher percentage content	535:561	a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%)	535:613	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	52	theme	protein	566:572	arg1	mass					638:641	a lower molecular mass	620:641	a lower molecular mass (17.54 × 106 g/mol)	620:661	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	3	52	theme	protein	566:572	arg1	g/mol					656:660	17.54 × 106 g/mol	644:660	17.54 × 106 g/mol	644:660	The results showed that L-TPS presented a higher percentage content of protein (2.33%) than commercial TPS (0.73-0.87%), and a lower molecular mass (17.54 × 106 g/mol).
37050384	1	53	theme	Tremella	195:202	arg1	TPS					230:232	TPS	230:232	TPS	230:232	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	1	53	theme	Tremella	195:202	arg1	polysaccharides					213:227	Tremella fuciform polysaccharides	195:227	Tremella fuciform polysaccharides (TPS)	195:233	The chemical composition, macromolecular characteristics, and structure of four types of Tremella fuciform polysaccharides (TPS) were analyzed, including one TPS that was extracted in the laboratory (L-TPS) and three commercial TPS.
37050384	5	54	dep	sizes	762:766	arg1	The					750:752	The	750:752	The	750:752	The droplet sizes and apparent viscosity of commercial TPS-stabilized emulsions were larger or higher in acidic environments.
37050384	8	55	theme	TPS	1111:1113	arg1	properties					1097:1106	the emulsifying properties	1081:1106	the emulsifying properties of TPS varied	1081:1120	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	8	55	theme	TPS	1111:1113	arg1	effects					1130:1136	the effects	1126:1136	the effects of pH on emulsion characteristics	1126:1170	The obtained results revealed that the emulsifying properties of TPS varied and the effects of pH on emulsion characteristics differed, as determined from the molecular mass, macromolecular characteristics, and structure.
37050384	9	56	theme	TPS	1325:1327	arg1	application					1310:1320	the application	1306:1320	the application of TPS	1306:1327	This research is useful for expanding the application of TPS as a novel food ingredient in emulsions.
37050384	9	56	theme	TPS	1325:1327	arg1	ingredient					1345:1354	a novel food ingredient	1332:1354	a novel food ingredient in emulsions	1332:1367	This research is useful for expanding the application of TPS as a novel food ingredient in emulsions.
37050384	4	57	theme	best	690:693	arg1	activity					707:714	the best emulsifying activity	686:714	the best emulsifying activity	686:714	Thus, L-TPS exhibited the best emulsifying activity but gave poor emulsion stability.
36761225	4	0	from	pathways	812:819	arg1	group					780:784	each group	775:784	each group	775:784	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	11	1	dep	alleviate	1942:1950	arg1	used					2027:2030	used	2027:2030	be used as a functional food adjuvant	2024:2060	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	11	2	dep	obesity	1956:1962	arg1	the					1952:1954	the	1952:1954	the	1952:1954	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	3	3	theme	epididymal	571:580	arg1	tissue					590:595	epididymal adipose tissue	571:595	epididymal adipose tissue of mice	571:603	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	1	4	theme	polysaccharides	180:194	arg1	structure					149:157	the structure	145:157	the structure of Pleurotus eryngii polysaccharides (PEPs)	145:201	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	10	5	from	changes	1790:1796	arg1	pathways					1811:1818	metabolic pathways	1801:1818	metabolic pathways such as that involved in the citric cycle (TCA cycle)	1801:1872	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	5	from	changes	1790:1796	arg1	that					1828:1831	that	1828:1831	that	1828:1831	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	4	6	theme	HFX	677:679	arg1	MS					701:702	MS	701:702	MS	701:702	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	6	theme	HFX	677:679	arg1	spectrometry					687:698	(QE) HFX -mass spectrometry	672:698	(QE) HFX -mass spectrometry (MS)	672:703	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	10	7	theme	citric	1849:1854	arg1	cycle					1856:1860	the citric cycle	1845:1860	the citric cycle (TCA cycle)	1845:1872	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	7	theme	citric	1849:1854	arg1	cycle					1867:1871	TCA cycle	1863:1871	TCA cycle	1863:1871	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	6	8	theme	rate	1110:1113	arg1	Tm					1128:1129	Tm	1128:1129	Tm	1128:1129	The PEPs began to degrade at 251°C (T0), while the maximum thermal degradation rate temperature (Tm) appeared at 300°C.
36761225	6	8	theme	rate	1110:1113	arg1	temperature					1115:1125	the maximum thermal degradation rate temperature	1078:1125	the maximum thermal degradation rate temperature (Tm)	1078:1130	The PEPs began to degrade at 251°C (T0), while the maximum thermal degradation rate temperature (Tm) appeared at 300°C.
36761225	3	9	theme	mice	600:603	arg1	tissue					590:595	epididymal adipose tissue	571:595	epididymal adipose tissue of mice	571:603	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	11	10	theme	high-fat	2152:2159	arg1	obesity					2174:2180	high-fat diet-induced obesity	2152:2180	high-fat diet-induced obesity	2152:2180	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	9	11	theme	hyperlipidemia-related	1452:1473	arg1	complications					1475:1487	hyperlipidemia-related complications	1452:1487	hyperlipidemia-related complications	1452:1487	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	3	12	theme	tissue	590:595	arg1	difference					557:566	the morphological difference	539:566	the morphological difference of epididymal adipose tissue of mice in each group	539:617	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	11	13	theme	PEPs	1923:1926	arg1	treatment					1928:1936	PEPs treatment	1923:1936	PEPs treatment	1923:1936	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	6	14	theme	degradation	1098:1108	arg1	rate					1110:1113	the maximum thermal degradation rate	1078:1113	the maximum thermal degradation rate temperature (Tm)	1078:1130	The PEPs began to degrade at 251°C (T0), while the maximum thermal degradation rate temperature (Tm) appeared at 300°C.
36761225	5	15	theme	%	984:984	arg1	acid					998:1001	0.21%galacturonic acid	980:1001	0.21%galacturonic acid	980:1001	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	15	theme	%	984:984	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	9	16	theme	metabolites	1366:1376	arg1	total					1344:1348	A total	1342:1348	A total of 29 different metabolites	1342:1376	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	9	16	theme	metabolites	1366:1376	arg1	therapy					1406:1412	adjunctive therapy	1395:1412	adjunctive therapy to PEPs	1395:1420	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	6	17	theme	maximum	1082:1088	arg1	rate					1110:1113	the maximum thermal degradation rate	1078:1113	the maximum thermal degradation rate temperature (Tm)	1078:1130	The PEPs began to degrade at 251°C (T0), while the maximum thermal degradation rate temperature (Tm) appeared at 300°C.
36761225	5	18	theme	0.30	885:888	arg1	%					889:889	%	889:889	%	889:889	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	7	19	theme	signifificant	1224:1236	arg1	activities					1252:1261	signifificant hypolipidemic activities	1224:1261	signifificant hypolipidemic activities	1224:1261	The results histopathological observation demonstrated that the PEPs had signifificant hypolipidemic activities.
36761225	5	20	theme	%	907:907	arg1	galactose					909:917	17.94% galactose	902:917	17.94% galactose	902:917	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	20	theme	%	907:907	arg1	xylose					942:947	1.24% xylose	936:947	1.24% xylose	936:947	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	20	theme	%	907:907	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	20	theme	%	907:907	arg1	glucose					927:933	53.49% glucose	920:933	53.49% glucose	920:933	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	21	theme	%	970:970	arg1	ribose					972:977	1.30% ribose	966:977	1.30% ribose	966:977	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	21	theme	%	970:970	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	9	22	theme	adjunctive	1395:1404	arg1	total					1344:1348	A total	1342:1348	A total of 29 different metabolites	1342:1376	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	9	22	theme	adjunctive	1395:1404	arg1	therapy					1406:1412	adjunctive therapy	1395:1412	adjunctive therapy to PEPs	1395:1420	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	11	23	dep	Conclusions	1875:1885	arg1	indicate					1909:1916	indicate	1909:1916	indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia	1909:2199	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	0	24	theme	diet-induced	73:84	arg1	mice					92:95	high-fat diet-induced obese mice	64:95	high-fat diet-induced obese mice	64:95	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	5	25	theme	%	1012:1012	arg1	acid					1025:1028	1.17% glucuronic acid	1008:1028	1.17% glucuronic acid	1008:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	25	theme	%	1012:1012	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	10	26	theme	high-fat	1712:1719	arg1	diet					1721:1724	a high-fat diet	1710:1724	a high-fat diet	1710:1724	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	5	27	theme	1.05	871:874	arg1	%					875:875	%	875:875	%	875:875	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	3	28	from	difference	557:566	arg1	group					613:617	each group	608:617	each group	608:617	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	4	29	used	used	709:712	arg2	chromatography					649:662	Ultrahigh performance liquid chromatography	620:662	Ultrahigh performance liquid chromatography (UHPLC)	620:670	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	29	used	used	709:712	arg2	MS					701:702	MS	701:702	MS	701:702	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	29	used	used	709:712	arg2	UHPLC					665:669	UHPLC	665:669	UHPLC	665:669	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	29	used	used	709:712	arg2	spectrometry					687:698	(QE) HFX -mass spectrometry	672:698	(QE) HFX -mass spectrometry (MS)	672:703	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	1	30	theme	Pleurotus	162:170	arg1	PEPs					197:200	PEPs	197:200	PEPs	197:200	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	1	30	theme	Pleurotus	162:170	arg1	polysaccharides					180:194	Pleurotus eryngii polysaccharides	162:194	Pleurotus eryngii polysaccharides (PEPs)	162:201	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	5	31	theme	53.49	920:924	arg1	%					925:925	%	925:925	%	925:925	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	11	32	theme	theoretical	2075:2085	arg1	basis					2087:2091	theoretical basis	2075:2091	theoretical basis	2075:2091	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	2	33	theme	monosaccharide	393:406	arg1	Fourier					421:427	Fourier	421:427	Fourier transform infrared spectroscopy and thermogravimetry	421:480	Methods The structure of PEPs were characterized by monosaccharide composition, Fourier transform infrared spectroscopy and thermogravimetry.
36761225	2	33	theme	monosaccharide	393:406	arg1	composition					408:418	monosaccharide composition	393:418	monosaccharide composition	393:418	Methods The structure of PEPs were characterized by monosaccharide composition, Fourier transform infrared spectroscopy and thermogravimetry.
36761225	9	34	theme	obesity	1440:1446	arg1	treatment					1427:1435	treatment	1427:1435	treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet	1427:1513	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	0	35	theme	Hypolipidemic	0:12	arg1	mechanism					14:22	Hypolipidemic mechanism	0:22	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice	0:95	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	5	36	theme	17.94	902:906	arg1	%					907:907	%	907:907	%	907:907	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	2	37	dep	Methods	341:347	arg1	structure					353:361	The structure	349:361	Methods The structure of PEPs	341:369	Methods The structure of PEPs were characterized by monosaccharide composition, Fourier transform infrared spectroscopy and thermogravimetry.
36761225	3	38	theme	animal	486:491	arg1	experiments					493:503	animal experiments	486:503	animal experiments	486:503	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	7	39	theme	histopathological	1163:1179	arg1	observation					1181:1191	histopathological observation	1163:1191	histopathological observation	1163:1191	The results histopathological observation demonstrated that the PEPs had signifificant hypolipidemic activities.
36761225	0	40	theme	eryngii	37:43	arg1	polysaccharides					45:59	Pleurotus eryngii polysaccharides	27:59	Pleurotus eryngii polysaccharides	27:59	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	7	41	dep	demonstrated	1193:1204	arg1	results					1155:1161	The results	1151:1161	The results	1151:1161	The results histopathological observation demonstrated that the PEPs had signifificant hypolipidemic activities.
36761225	10	42	theme	amino	1740:1744	arg1	metabolism					1751:1760	amino acid metabolism	1740:1760	amino acid metabolism	1740:1760	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	4	43	theme	metabolic	802:810	arg1	pathways					812:819	the related metabolic pathways	790:819	the related metabolic pathways	790:819	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	1	44	theme	metabonomic	319:329	arg1	analysis					331:338	metabonomic analysis	319:338	metabonomic analysis	319:338	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	1	45	dep	characterized	207:219	arg1	Objective					120:128	Objective	120:128	Objective In this study	120:142	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	5	46	dep	Results	822:828	arg1	contained					839:847	contained	839:847	contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	839:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	47	theme	23.32	950:954	arg1	%					955:955	%	955:955	%	955:955	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	10	48	theme	glycerol	1589:1596	arg1	phospholipids					1598:1610	glycerol phospholipids	1589:1610	glycerol phospholipids	1589:1610	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	1	49	from	Objective	120:128	arg1	study					138:142	this study	133:142	this study	133:142	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	7	50	contain	had	1220:1222	arg1	PEPs					1215:1218	the PEPs	1211:1218	the PEPs	1211:1218	The results histopathological observation demonstrated that the PEPs had signifificant hypolipidemic activities.
36761225	7	50	contain	had	1220:1222	arg2	activities					1252:1261	signifificant hypolipidemic activities	1224:1261	signifificant hypolipidemic activities	1224:1261	The results histopathological observation demonstrated that the PEPs had signifificant hypolipidemic activities.
36761225	5	51	theme	1.24	936:939	arg1	%					940:940	%	940:940	%	940:940	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	11	52	theme	diet-induced	2161:2172	arg1	obesity					2174:2180	high-fat diet-induced obesity	2152:2180	high-fat diet-induced obesity	2152:2180	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	4	53	theme	Ultrahigh	620:628	arg1	UHPLC					665:669	UHPLC	665:669	UHPLC	665:669	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	53	theme	Ultrahigh	620:628	arg1	chromatography					649:662	Ultrahigh performance liquid chromatography	620:662	Ultrahigh performance liquid chromatography (UHPLC)	620:670	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	8	54	theme	PEPs	1270:1273	arg1	intervention					1275:1286	PEPs intervention	1270:1286	PEPs intervention	1270:1286	After PEPs intervention, the metabolic profile of mice changed significantly.
36761225	2	55	theme	PEPs	366:369	arg1	structure					353:361	The structure	349:361	Methods The structure of PEPs	341:369	Methods The structure of PEPs were characterized by monosaccharide composition, Fourier transform infrared spectroscopy and thermogravimetry.
36761225	4	56	theme	liquid	642:647	arg1	UHPLC					665:669	UHPLC	665:669	UHPLC	665:669	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	56	theme	liquid	642:647	arg1	chromatography					649:662	Ultrahigh performance liquid chromatography	620:662	Ultrahigh performance liquid chromatography (UHPLC)	620:670	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	10	57	theme	other	1617:1621	arg1	compounds					1634:1642	other endogenous compounds	1617:1642	other endogenous compounds	1617:1642	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	1	58	theme	PEP	243:245	arg1	mechanism					230:238	the mechanism	226:238	the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet	226:300	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	10	59	from	metabolism	1751:1760	arg1	pathways					1811:1818	metabolic pathways	1801:1818	metabolic pathways such as that involved in the citric cycle (TCA cycle)	1801:1872	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	59	from	metabolism	1751:1760	arg1	that					1828:1831	that	1828:1831	that	1828:1831	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	11	60	theme	high-fat	1995:2002	arg1	diet					2004:2007	a high-fat diet	1993:2007	a high-fat diet	1993:2007	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	2	61	dep	transform	429:437	arg1	infrared					439:446	infrared	439:446	transform infrared spectroscopy and thermogravimetry	429:480	Methods The structure of PEPs were characterized by monosaccharide composition, Fourier transform infrared spectroscopy and thermogravimetry.
36761225	3	62	theme	morphological	543:555	arg1	difference					557:566	the morphological difference	539:566	the morphological difference of epididymal adipose tissue of mice in each group	539:617	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	1	63	theme	eryngii	172:178	arg1	PEPs					197:200	PEPs	197:200	PEPs	197:200	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	1	63	theme	eryngii	172:178	arg1	polysaccharides					180:194	Pleurotus eryngii polysaccharides	162:194	Pleurotus eryngii polysaccharides (PEPs)	162:201	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	10	64	from	metabolism	1774:1783	arg1	pathways					1811:1818	metabolic pathways	1801:1818	metabolic pathways such as that involved in the citric cycle (TCA cycle)	1801:1872	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	64	from	metabolism	1774:1783	arg1	that					1828:1831	that	1828:1831	that	1828:1831	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	2	65	dep	Fourier	421:427	arg1	transform					429:437	transform	429:437	transform infrared spectroscopy and thermogravimetry	429:480	Methods The structure of PEPs were characterized by monosaccharide composition, Fourier transform infrared spectroscopy and thermogravimetry.
36761225	3	66	theme	adipose	582:588	arg1	tissue					590:595	epididymal adipose tissue	571:595	epididymal adipose tissue of mice	571:603	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	5	67	theme	0.21	980:983	arg1	%					984:984	%	984:984	%	984:984	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	9	68	theme	different	1356:1364	arg1	metabolites					1366:1376	29 different metabolites	1353:1376	29 different metabolites	1353:1376	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	6	69	theme	thermal	1090:1096	arg1	rate					1110:1113	the maximum thermal degradation rate	1078:1113	the maximum thermal degradation rate temperature (Tm)	1078:1130	The PEPs began to degrade at 251°C (T0), while the maximum thermal degradation rate temperature (Tm) appeared at 300°C.
36761225	10	70	theme	TCA	1863:1865	arg1	cycle					1856:1860	the citric cycle	1845:1860	the citric cycle (TCA cycle)	1845:1872	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	70	theme	TCA	1863:1865	arg1	cycle					1867:1871	TCA cycle	1863:1871	TCA cycle	1863:1871	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	1	71	from	mechanism	230:238	arg1	hyperlipidemia					262:275	hyperlipidemia	262:275	hyperlipidemia	262:275	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	1	71	from	mechanism	230:238	arg1	obesity					250:256	obesity	250:256	obesity	250:256	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	5	72	theme	galacturonic	985:996	arg1	acid					998:1001	0.21%galacturonic acid	980:1001	0.21%galacturonic acid	980:1001	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	72	theme	galacturonic	985:996	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	8	73	theme	metabolic	1293:1301	arg1	profile					1303:1309	the metabolic profile	1289:1309	the metabolic profile of mice	1289:1317	After PEPs intervention, the metabolic profile of mice changed significantly.
36761225	5	74	theme	1.30	966:969	arg1	%					970:970	%	970:970	%	970:970	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	75	theme	%	889:889	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	75	theme	%	889:889	arg1	arabinose					891:899	0.30% arabinose	885:899	0.30% arabinose	885:899	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	11	76	theme	presented	1891:1899	arg1	results					1901:1907	The presented results	1887:1907	The presented results	1887:1907	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	11	77	dep	prevention	2124:2133	arg1	the					2120:2122	the	2120:2122	the	2120:2122	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	7	78	theme	hypolipidemic	1238:1250	arg1	activities					1252:1261	signifificant hypolipidemic activities	1224:1261	signifificant hypolipidemic activities	1224:1261	The results histopathological observation demonstrated that the PEPs had signifificant hypolipidemic activities.
36761225	0	79	theme	obese	86:90	arg1	mice					92:95	high-fat diet-induced obese mice	64:95	high-fat diet-induced obese mice	64:95	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	4	80	theme	-mass	681:685	arg1	MS					701:702	MS	701:702	MS	701:702	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	80	theme	-mass	681:685	arg1	spectrometry					687:698	(QE) HFX -mass spectrometry	672:698	(QE) HFX -mass spectrometry (MS)	672:703	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	5	81	theme	glucuronic	1014:1023	arg1	acid					1025:1028	1.17% glucuronic acid	1008:1028	1.17% glucuronic acid	1008:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	81	theme	glucuronic	1014:1023	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	82	theme	%	875:875	arg1	fucose					877:882	1.05% fucose	871:882	1.05% fucose	871:882	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	82	theme	%	875:875	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	0	83	from	mechanism	14:22	arg1	mice					92:95	high-fat diet-induced obese mice	64:95	high-fat diet-induced obese mice	64:95	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	3	84	used	used	523:526	arg2	staining					510:517	H&E staining	506:517	H&E staining	506:517	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	4	85	from	serum	758:762	arg1	group					780:784	each group	775:784	each group	775:784	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	11	86	theme	technical	2097:2105	arg1	guidance					2107:2114	technical guidance	2097:2114	technical guidance	2097:2114	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	5	87	contain	contained	839:847	arg2	mannose					957:963	23.32% mannose	950:963	23.32% mannose	950:963	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg2	acid					998:1001	0.21%galacturonic acid	980:1001	0.21%galacturonic acid	980:1001	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg2	fucose					877:882	1.05% fucose	871:882	1.05% fucose	871:882	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg2	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg2	galactose					909:917	17.94% galactose	902:917	17.94% galactose	902:917	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg2	arabinose					891:899	0.30% arabinose	885:899	0.30% arabinose	885:899	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg2	acid					1025:1028	1.17% glucuronic acid	1008:1028	1.17% glucuronic acid	1008:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg1	PEPs					834:837	The PEPs	830:837	The PEPs	830:837	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	87	contain	contained	839:847	arg2	ribose					972:977	1.30% ribose	966:977	1.30% ribose	966:977	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	4	88	theme	metabolites	743:753	arg1	difference					729:738	the difference	725:738	the difference of metabolites in serum of mice in each group and the related metabolic pathways	725:819	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	5	89	theme	%	925:925	arg1	galactose					909:917	17.94% galactose	902:917	17.94% galactose	902:917	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	89	theme	%	925:925	arg1	glucose					927:933	53.49% glucose	920:933	53.49% glucose	920:933	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	90	theme	1.17	1008:1011	arg1	%					1012:1012	%	1012:1012	%	1012:1012	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	4	91	from	difference	729:738	arg1	pathways					812:819	the related metabolic pathways	790:819	the related metabolic pathways	790:819	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	91	from	difference	729:738	arg1	serum					758:762	serum	758:762	serum of mice in each group	758:784	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	10	92	theme	fatty	1763:1767	arg1	metabolism					1774:1783	fatty acid metabolism	1763:1783	fatty acid metabolism	1763:1783	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	93	theme	amino	1542:1546	arg1	acids					1548:1552	amino acids	1542:1552	amino acids	1542:1552	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	0	94	theme	Pleurotus	27:35	arg1	polysaccharides					45:59	Pleurotus eryngii polysaccharides	27:59	Pleurotus eryngii polysaccharides	27:59	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	9	95	theme	complications	1475:1487	arg1	treatment					1427:1435	treatment	1427:1435	treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet	1427:1513	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	0	96	theme	polysaccharides	45:59	arg1	mechanism					14:22	Hypolipidemic mechanism	0:22	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice	0:95	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	1	97	theme	high-fat	288:295	arg1	diet					297:300	high-fat diet	288:300	high-fat diet	288:300	Objective In this study, the structure of Pleurotus eryngii polysaccharides (PEPs) was characterized, and the mechanism of PEP on obesity and hyperlipidemia induced by high-fat diet was evaluated by metabonomic analysis.
36761225	10	98	theme	unsaturated	1555:1565	arg1	acids					1573:1577	unsaturated fatty acids	1555:1577	unsaturated fatty acids	1555:1577	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	99	theme	acid	1746:1749	arg1	metabolism					1751:1760	amino acid metabolism	1740:1760	amino acid metabolism	1740:1760	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	5	100	dep	monosaccharides	854:868	arg1	xylose					942:947	1.24% xylose	936:947	1.24% xylose	936:947	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	acid					1025:1028	1.17% glucuronic acid	1008:1028	1.17% glucuronic acid	1008:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	arabinose					891:899	0.30% arabinose	885:899	0.30% arabinose	885:899	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	acid					998:1001	0.21%galacturonic acid	980:1001	0.21%galacturonic acid	980:1001	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	ribose					972:977	1.30% ribose	966:977	1.30% ribose	966:977	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	glucose					927:933	53.49% glucose	920:933	53.49% glucose	920:933	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	fucose					877:882	1.05% fucose	871:882	1.05% fucose	871:882	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	mannose					957:963	23.32% mannose	950:963	23.32% mannose	950:963	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	galactose					909:917	17.94% galactose	902:917	17.94% galactose	902:917	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	100	dep	monosaccharides	854:868	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	10	101	theme	fatty	1567:1571	arg1	acids					1573:1577	unsaturated fatty acids	1555:1577	unsaturated fatty acids	1555:1577	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	0	102	theme	high-fat	64:71	arg1	mice					92:95	high-fat diet-induced obese mice	64:95	high-fat diet-induced obese mice	64:95	Hypolipidemic mechanism of Pleurotus eryngii polysaccharides in high-fat diet-induced obese mice based on metabolomics.
36761225	5	103	theme	%	955:955	arg1	monosaccharides					854:868	nine monosaccharides	849:868	nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid	849:1028	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	103	theme	%	955:955	arg1	mannose					957:963	23.32% mannose	950:963	23.32% mannose	950:963	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	11	104	dep	basis	2087:2091	arg1	a					2073:2073	a	2073:2073	a	2073:2073	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	4	105	theme	related	794:800	arg1	pathways					812:819	the related metabolic pathways	790:819	the related metabolic pathways	790:819	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	9	106	theme	high-fat	1501:1508	arg1	diet					1510:1513	a high-fat diet	1499:1513	a high-fat diet	1499:1513	A total of 29 different metabolites were selected as adjunctive therapy to PEPs, for treatment of obesity and hyperlipidemia-related complications caused by a high-fat diet.
36761225	11	107	theme	hyperlipidemia	2186:2199	arg1	treatment					2139:2147	treatment	2139:2147	treatment	2139:2147	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	11	107	theme	hyperlipidemia	2186:2199	arg1	prevention					2124:2133	prevention	2124:2133	prevention	2124:2133	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	4	108	theme	performance	630:640	arg1	UHPLC					665:669	UHPLC	665:669	UHPLC	665:669	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	108	theme	performance	630:640	arg1	chromatography					649:662	Ultrahigh performance liquid chromatography	620:662	Ultrahigh performance liquid chromatography (UHPLC)	620:670	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	10	109	theme	acid	1769:1772	arg1	metabolism					1774:1783	fatty acid metabolism	1763:1783	fatty acid metabolism	1763:1783	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	4	110	theme	mice	767:770	arg1	pathways					812:819	the related metabolic pathways	790:819	the related metabolic pathways	790:819	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	4	110	theme	mice	767:770	arg1	serum					758:762	serum	758:762	serum of mice in each group	758:784	Ultrahigh performance liquid chromatography (UHPLC)-(QE) HFX -mass spectrometry (MS) was used to analyze the difference of metabolites in serum of mice in each group and the related metabolic pathways.
36761225	3	111	theme	H&E	506:508	arg1	staining					510:517	H&E staining	506:517	H&E staining	506:517	In animal experiments, H&E staining was used to observe the morphological difference of epididymal adipose tissue of mice in each group.
36761225	5	112	theme	%	940:940	arg1	galactose					909:917	17.94% galactose	902:917	17.94% galactose	902:917	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	5	112	theme	%	940:940	arg1	xylose					942:947	1.24% xylose	936:947	1.24% xylose	936:947	Results The PEPs contained nine monosaccharides: 1.05% fucose, 0.30% arabinose, 17.94% galactose, 53.49% glucose, 1.24% xylose, 23.32% mannose, 1.30% ribose, 0.21%galacturonic acid, and 1.17% glucuronic acid.
36761225	11	113	theme	obesity	2174:2180	arg1	treatment					2139:2147	treatment	2139:2147	treatment	2139:2147	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	11	113	theme	obesity	2174:2180	arg1	prevention					2124:2133	prevention	2124:2133	prevention	2124:2133	Conclusions The presented results indicate that PEPs treatment can alleviate the obesity and hyperlipidemia caused by a high-fat diet and, thus, may be used as a functional food adjuvant, providing a theoretical basis and technical guidance for the prevention and treatment of high-fat diet-induced obesity and hyperlipidemia.
36761225	10	114	theme	metabolic	1801:1809	arg1	pathways					1811:1818	metabolic pathways	1801:1818	metabolic pathways such as that involved in the citric cycle (TCA cycle)	1801:1872	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	10	114	theme	metabolic	1801:1809	arg1	that					1828:1831	that	1828:1831	that	1828:1831	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
36761225	8	115	theme	mice	1314:1317	arg1	profile					1303:1309	the metabolic profile	1289:1309	the metabolic profile of mice	1289:1317	After PEPs intervention, the metabolic profile of mice changed significantly.
36761225	10	116	theme	endogenous	1623:1632	arg1	compounds					1634:1642	other endogenous compounds	1617:1642	other endogenous compounds	1617:1642	These metabolites include amino acids, unsaturated fatty acids, choline, glycerol phospholipids, and other endogenous compounds, which can prevent and treat obesity and hyperlipidemia caused by a high-fat diet by regulating amino acid metabolism, fatty acid metabolism, and changes in metabolic pathways such as that involved in the citric cycle (TCA cycle).
34788844	2	0	contain	contain	325:331	arg2	monosaccharides					419:433	monosaccharides	419:433	monosaccharides	419:433	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	0	contain	contain	325:331	arg1	droplets					306:313	extracellular droplets	292:313	extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	292:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	0	contain	contain	325:331	arg2	concentration					340:352	a high concentration	333:352	a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	333:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	4	1	theme	new	684:686	arg1	approach					688:695	a new approach	682:695	a new approach for the microbial generation of oil from waste	682:742	The results raise the possibility that these droplets could be harvested offering a new approach for the microbial generation of oil from waste.
34788844	3	2	dep	concentration	460:472	arg1	The					456:458	The	456:458	The	456:458	The concentration and composition of the fatty acids varied according to the age of the droplet and the feedstock used for growth of the fungi.
34788844	4	3	theme	oil	729:731	arg1	generation					715:724	the microbial generation	701:724	the microbial generation of oil from waste	701:742	The results raise the possibility that these droplets could be harvested offering a new approach for the microbial generation of oil from waste.
34788844	3	4	theme	droplet	544:550	arg1	age					533:535	the age	529:535	the age of the droplet	529:550	The concentration and composition of the fatty acids varied according to the age of the droplet and the feedstock used for growth of the fungi.
34788844	3	4	theme	droplet	544:550	arg1	feedstock					560:568	the feedstock	556:568	the feedstock used for growth of the fungi	556:597	The concentration and composition of the fatty acids varied according to the age of the droplet and the feedstock used for growth of the fungi.
34788844	3	5	theme	fatty	497:501	arg1	acids					503:507	the fatty acids	493:507	the fatty acids	493:507	The concentration and composition of the fatty acids varied according to the age of the droplet and the feedstock used for growth of the fungi.
34788844	4	6	from	waste	738:742	arg1	generation					715:724	the microbial generation	701:724	the microbial generation of oil from waste	701:742	The results raise the possibility that these droplets could be harvested offering a new approach for the microbial generation of oil from waste.
34788844	1	7	theme	fatty	160:164	arg1	acids					166:170	fatty acids	160:170	fatty acids	160:170	Fungi can acquire and store nutrients through decomposing and converting organic matter into fatty acids.
34788844	2	8	theme	unsaturated	384:394	arg1	acids					402:406	unsaturated fatty acids	384:406	unsaturated fatty acids	384:406	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	0	9	theme	acid	6:9	arg1	secretion					11:19	Fatty acid secretion	0:19	Fatty acid secretion by the white-rot fungus, Trametes versicolor.	0:65	Fatty acid secretion by the white-rot fungus, Trametes versicolor.
34788844	0	10	theme	Fatty	0:4	arg1	secretion					11:19	Fatty acid secretion	0:19	Fatty acid secretion by the white-rot fungus, Trametes versicolor.	0:65	Fatty acid secretion by the white-rot fungus, Trametes versicolor.
34788844	2	11	contain	has	265:267	arg2	ability					273:279	the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	269:453	the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	269:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	11	contain	has	265:267	arg1	versicolor					254:263	the white-rot fungus Trametes versicolor	224:263	the white-rot fungus Trametes versicolor	224:263	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	12	theme	acids	374:378	arg1	polysaccharides					439:453	polysaccharides	439:453	polysaccharides	439:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	12	theme	acids	374:378	arg1	monosaccharides					419:433	monosaccharides	419:433	monosaccharides	419:433	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	12	theme	acids	374:378	arg1	concentration					340:352	a high concentration	333:352	a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	333:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	3	13	theme	fungi	593:597	arg1	growth					579:584	growth	579:584	growth of the fungi	579:597	The concentration and composition of the fatty acids varied according to the age of the droplet and the feedstock used for growth of the fungi.
34788844	2	14	theme	extracellular	292:304	arg1	droplets					306:313	extracellular droplets	292:313	extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	292:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	1	15	theme	store	89:93	arg1	nutrients					95:103	and store nutrients	85:103	nutrients	95:103	Fungi can acquire and store nutrients through decomposing and converting organic matter into fatty acids.
34788844	0	16	theme	white-rot	28:36	arg1	fungus					38:43	the white-rot fungus	24:43	the white-rot fungus	24:43	Fatty acid secretion by the white-rot fungus, Trametes versicolor.
34788844	0	16	theme	white-rot	28:36	arg1	versicolor					55:64	Trametes versicolor	46:64	Trametes versicolor	46:64	Fatty acid secretion by the white-rot fungus, Trametes versicolor.
34788844	2	17	theme	fatty	396:400	arg1	acids					402:406	unsaturated fatty acids	384:406	unsaturated fatty acids	384:406	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	18	theme	acids	402:406	arg1	polysaccharides					439:453	polysaccharides	439:453	polysaccharides	439:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	18	theme	acids	402:406	arg1	monosaccharides					419:433	monosaccharides	419:433	monosaccharides	419:433	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	18	theme	acids	402:406	arg1	concentration					340:352	a high concentration	333:352	a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	333:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	19	theme	high	335:338	arg1	concentration					340:352	a high concentration	333:352	a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides	333:453	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	20	theme	first	208:212	arg1	time					214:217	the first time	204:217	the first time	204:217	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	3	21	theme	acids	503:507	arg1	concentration					460:472	concentration	460:472	concentration	460:472	The concentration and composition of the fatty acids varied according to the age of the droplet and the feedstock used for growth of the fungi.
34788844	3	21	theme	acids	503:507	arg1	composition					478:488	composition	478:488	composition	478:488	The concentration and composition of the fatty acids varied according to the age of the droplet and the feedstock used for growth of the fungi.
34788844	4	22	theme	microbial	705:713	arg1	generation					715:724	the microbial generation	701:724	the microbial generation of oil from waste	701:742	The results raise the possibility that these droplets could be harvested offering a new approach for the microbial generation of oil from waste.
34788844	2	23	theme	Trametes	245:252	arg1	versicolor					254:263	the white-rot fungus Trametes versicolor	224:263	the white-rot fungus Trametes versicolor	224:263	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	1	24	theme	organic	140:146	arg1	matter					148:153	organic matter	140:153	organic matter	140:153	Fungi can acquire and store nutrients through decomposing and converting organic matter into fatty acids.
34788844	2	25	theme	fungus	238:243	arg1	versicolor					254:263	the white-rot fungus Trametes versicolor	224:263	the white-rot fungus Trametes versicolor	224:263	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	26	theme	fatty	368:372	arg1	acids					374:378	long-chain fatty acids	357:378	long-chain fatty acids	357:378	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	0	27	theme	Trametes	46:53	arg1	fungus					38:43	the white-rot fungus	24:43	the white-rot fungus	24:43	Fatty acid secretion by the white-rot fungus, Trametes versicolor.
34788844	0	27	theme	Trametes	46:53	arg1	versicolor					55:64	Trametes versicolor	46:64	Trametes versicolor	46:64	Fatty acid secretion by the white-rot fungus, Trametes versicolor.
34788844	2	28	theme	white-rot	228:236	arg1	versicolor					254:263	the white-rot fungus Trametes versicolor	224:263	the white-rot fungus Trametes versicolor	224:263	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
34788844	2	29	theme	long-chain	357:366	arg1	acids					374:378	long-chain fatty acids	357:378	long-chain fatty acids	357:378	This research demonstrates for the first time that the white-rot fungus Trametes versicolor has the ability to secrete extracellular droplets which can contain a high concentration of long-chain fatty acids and unsaturated fatty acids as well as monosaccharides and polysaccharides.
36074808	0	0	theme	Electron	92:99	arg1	Dissociation					109:120	Hot Electron Capture Dissociation	88:120	Hot Electron Capture Dissociation	88:120	Localization of Multiple <i>O</i>-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
36074808	5	1	theme	<i>O</i>-glycopeptides	849:870	arg1	<i>O</i>-glycopeptides					814:835	Sixty-nine <i>O</i>-glycopeptides	803:835	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest	803:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	3	2	gly	glycosylated	452:463	arg1	species					465:471	low abundant glycosylated species	439:471	low abundant glycosylated species	439:471	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	5	3	theme	relative	925:932	arg1	abundance					934:942	a relative abundance	923:942	a relative abundance range	923:948	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	1	4	theme	comprehensive	156:168	arg1	profile					170:176	a comprehensive profile	154:176	a comprehensive profile of multiple glycosylations in glycopeptide isoforms	154:228	We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms.
36074808	0	5	theme	Hot	88:90	arg1	Dissociation					109:120	Hot Electron Capture Dissociation	88:120	Hot Electron Capture Dissociation	88:120	Localization of Multiple <i>O</i>-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
36074808	5	6	theme	tryptic	875:881	arg1	digest					897:902	tryptic bovine fetuin digest	875:902	tryptic bovine fetuin digest	875:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	3	7	theme	dissociation	557:568	arg1	device					570:575	an efficient electron-activated dissociation device	525:575	an efficient electron-activated dissociation device to perform hot ECD	525:594	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	1	8	from	profile	170:176	arg1	isoforms					221:228	glycopeptide isoforms	208:228	glycopeptide isoforms	208:228	We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms.
36074808	2	9	gly	glycopeptides	313:325	arg2	glycopeptides					313:325	isomeric glycopeptides	304:325	isomeric glycopeptides using hot electron capture dissociation (hot ECD)	304:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	10	theme	isomeric	304:311	arg1	glycopeptides					313:325	isomeric glycopeptides	304:325	isomeric glycopeptides using hot electron capture dissociation (hot ECD)	304:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	11	gly	<i>O</i>-glycoforms	281:299	arg2	glycopeptides					313:325	isomeric glycopeptides	304:325	isomeric glycopeptides using hot electron capture dissociation (hot ECD)	304:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	5	12	theme	abundance	934:942	arg1	range					944:948	a relative abundance range	923:948	a relative abundance range	923:948	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	5	13	gly	<i>O</i>-glycopeptides	849:870	arg2	<i>O</i>-glycopeptides					849:870	the long <i>O</i>-glycopeptides	840:870	the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest	840:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	5	14	theme	bovine	883:888	arg1	digest					897:902	tryptic bovine fetuin digest	875:902	tryptic bovine fetuin digest	875:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	5	15	theme	sialylated	983:992	arg1	glycans					994:1000	sialylated glycans	983:1000	sialylated glycans with Neu5Ac and Neu5Gc	983:1023	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	1	16	gly	glycopeptide	208:219	arg2	glycopeptide					208:219	glycopeptide isoforms	208:228	glycopeptide isoforms	208:228	We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms.
36074808	2	17	theme	<i>O</i>-glycoforms	281:299	arg1	abundances					259:268	abundances	259:268	abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD)	259:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	1	18	theme	multiple	181:188	arg1	glycosylations					190:203	multiple glycosylations	181:203	multiple glycosylations	181:203	We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms.
36074808	5	19	with	glycans	994:1000	arg1	Neu5Gc					1018:1023	Neu5Gc	1018:1023	Neu5Gc	1018:1023	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	5	19	with	glycans	994:1000	arg1	Neu5Ac					1007:1012	Neu5Ac	1007:1012	Neu5Ac	1007:1012	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	2	20	theme	capture	346:352	arg1	dissociation					354:365	hot electron capture dissociation	333:365	hot electron capture dissociation (hot ECD)	333:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	20	theme	capture	346:352	arg1	ECD					372:374	hot ECD	368:374	hot ECD	368:374	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	1	21	theme	glycosylations	190:203	arg1	profile					170:176	a comprehensive profile	154:176	a comprehensive profile of multiple glycosylations in glycopeptide isoforms	154:228	We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms.
36074808	3	22	dep	glycosylated	452:463	arg1	abundant					443:450	abundant	443:450	abundant	443:450	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	2	23	theme	electron	337:344	arg1	dissociation					354:365	hot electron capture dissociation	333:365	hot electron capture dissociation (hot ECD)	333:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	23	theme	electron	337:344	arg1	ECD					372:374	hot ECD	368:374	hot ECD	368:374	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	24	from	abundances	259:268	arg1	glycopeptides					313:325	isomeric glycopeptides	304:325	isomeric glycopeptides using hot electron capture dissociation (hot ECD)	304:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	25	theme	mass	409:412	arg1	spectrometry					414:425	liquid chromatography-tandem mass spectrometry	380:425	liquid chromatography-tandem mass spectrometry	380:425	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	0	26	theme	<i>O</i>-Linked	25:39	arg1	Glycans					41:47	Multiple <i>O</i>-Linked Glycans	16:47	Multiple <i>O</i>-Linked Glycans	16:47	Localization of Multiple <i>O</i>-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
36074808	3	27	theme	monitoring	668:677	arg1	workflows					679:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	2	28	theme	hot	333:335	arg1	dissociation					354:365	hot electron capture dissociation	333:365	hot electron capture dissociation (hot ECD)	333:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	28	theme	hot	333:335	arg1	ECD					372:374	hot ECD	368:374	hot ECD	368:374	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	5	29	dep	0.2	962:964	arg1	to					959:960	to	959:960	to	959:960	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	1	30	theme	glycopeptide	208:219	arg1	isoforms					221:228	glycopeptide isoforms	208:228	glycopeptide isoforms	208:228	We describe a method to obtain a comprehensive profile of multiple glycosylations in glycopeptide isoforms.
36074808	0	31	theme	Multiple	16:23	arg1	Glycans					41:47	Multiple <i>O</i>-Linked Glycans	16:47	Multiple <i>O</i>-Linked Glycans	16:47	Localization of Multiple <i>O</i>-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
36074808	5	32	from	<i>O</i>-glycopeptides	814:835	arg1	digest					897:902	tryptic bovine fetuin digest	875:902	tryptic bovine fetuin digest	875:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	5	33	gly	<i>O</i>-glycopeptides	814:835	arg1	<i>O</i>-glycopeptides					849:870	the long <i>O</i>-glycopeptides	840:870	the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest	840:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	5	33	gly	<i>O</i>-glycopeptides	814:835	arg2	<i>O</i>-glycopeptides					814:835	Sixty-nine <i>O</i>-glycopeptides	803:835	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest	803:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	3	34	theme	7600	499:502	arg1	system					504:509	a ZenoTOF 7600 system	489:509	a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD	489:594	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	3	35	theme	electron-activated	538:555	arg1	device					570:575	an efficient electron-activated dissociation device	525:575	an efficient electron-activated dissociation device to perform hot ECD	525:594	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	3	36	theme	efficient	528:536	arg1	device					570:575	an efficient electron-activated dissociation device	525:575	an efficient electron-activated dissociation device to perform hot ECD	525:594	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	3	37	theme	multiple	650:657	arg1	workflows					679:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	4	38	theme	trap	708:711	arg1	pulsing					713:719	Zeno trap pulsing	703:719	Zeno trap pulsing	703:719	In addition, Zeno trap pulsing was activated to enhance the sensitivity of the time-of-flight mass spectrometer.
36074808	0	39	theme	Glycans	41:47	arg1	Localization					0:11	Localization	0:11	Localization of Multiple <i>O</i>-Linked Glycans	0:47	Localization of Multiple <i>O</i>-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
36074808	3	40	theme	glycosylated	452:463	arg1	species					465:471	low abundant glycosylated species	439:471	low abundant glycosylated species	439:471	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	5	41	theme	Sixty-nine	803:812	arg1	<i>O</i>-glycopeptides					814:835	Sixty-nine <i>O</i>-glycopeptides	803:835	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest	803:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	2	42	theme	wide	245:248	arg1	range					250:254	a wide range	243:254	a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD)	243:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	4	43	theme	Zeno	703:706	arg1	pulsing					713:719	Zeno trap pulsing	703:719	Zeno trap pulsing	703:719	In addition, Zeno trap pulsing was activated to enhance the sensitivity of the time-of-flight mass spectrometer.
36074808	0	44	theme	Isomeric	62:69	arg1	Glycopeptides					71:83	Isomeric Glycopeptides	62:83	Isomeric Glycopeptides	62:83	Localization of Multiple <i>O</i>-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
36074808	5	45	theme	long	844:847	arg1	<i>O</i>-glycopeptides					849:870	the long <i>O</i>-glycopeptides	840:870	the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest	840:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	3	46	theme	scheduled	624:632	arg1	workflows					679:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	4	47	theme	time-of-flight	769:782	arg1	spectrometer					789:800	the time-of-flight mass spectrometer	765:800	the time-of-flight mass spectrometer	765:800	In addition, Zeno trap pulsing was activated to enhance the sensitivity of the time-of-flight mass spectrometer.
36074808	3	48	theme	low	439:441	arg1	species					465:471	low abundant glycosylated species	439:471	low abundant glycosylated species	439:471	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	5	49	from	<i>O</i>-glycopeptides	849:870	arg1	digest					897:902	tryptic bovine fetuin digest	875:902	tryptic bovine fetuin digest	875:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	3	50	theme	high-resolution	634:648	arg1	workflows					679:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	5	51	from	digest	897:902	arg1	<i>O</i>-glycopeptides					814:835	Sixty-nine <i>O</i>-glycopeptides	803:835	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest	803:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	2	52	theme	hot	368:370	arg1	dissociation					354:365	hot electron capture dissociation	333:365	hot electron capture dissociation (hot ECD)	333:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	2	52	theme	hot	368:370	arg1	ECD					372:374	hot ECD	368:374	hot ECD	368:374	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	3	53	theme	targeted	612:619	arg1	workflows					679:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	5	54	gly	sialylated	983:992	arg1	glycans					994:1000	sialylated glycans	983:1000	sialylated glycans with Neu5Ac and Neu5Gc	983:1023	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	2	55	theme	various	273:279	arg1	<i>O</i>-glycoforms					281:299	various <i>O</i>-glycoforms	273:299	various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD)	273:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	3	56	theme	reaction	659:666	arg1	workflows					679:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	targeted or scheduled high-resolution multiple reaction monitoring workflows	612:687	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	3	57	theme	system	504:509	arg1	prototype					476:484	a prototype	474:484	a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD	474:594	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	2	58	from	<i>O</i>-glycoforms	281:299	arg1	glycopeptides					313:325	isomeric glycopeptides	304:325	isomeric glycopeptides using hot electron capture dissociation (hot ECD)	304:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	3	59	theme	hot	588:590	arg1	ECD					592:594	hot ECD	588:594	hot ECD	588:594	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	4	60	theme	mass	784:787	arg1	spectrometer					789:800	the time-of-flight mass spectrometer	765:800	the time-of-flight mass spectrometer	765:800	In addition, Zeno trap pulsing was activated to enhance the sensitivity of the time-of-flight mass spectrometer.
36074808	2	61	from	glycopeptides	313:325	arg1	abundances					259:268	abundances	259:268	abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD)	259:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	4	62	theme	spectrometer	789:800	arg1	sensitivity					750:760	the sensitivity	746:760	the sensitivity of the time-of-flight mass spectrometer	746:800	In addition, Zeno trap pulsing was activated to enhance the sensitivity of the time-of-flight mass spectrometer.
36074808	2	63	theme	abundances	259:268	arg1	range					250:254	a wide range	243:254	a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD)	243:375	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	3	64	theme	ZenoTOF	491:497	arg1	system					504:509	a ZenoTOF 7600 system	489:509	a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD	489:594	To capture low abundant glycosylated species, a prototype of a ZenoTOF 7600 system incorporating an efficient electron-activated dissociation device to perform hot ECD was operated in targeted or scheduled high-resolution multiple reaction monitoring workflows.
36074808	2	65	theme	chromatography-tandem	387:407	arg1	spectrometry					414:425	liquid chromatography-tandem mass spectrometry	380:425	liquid chromatography-tandem mass spectrometry	380:425	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	5	66	theme	fetuin	890:895	arg1	digest					897:902	tryptic bovine fetuin digest	875:902	tryptic bovine fetuin digest	875:902	Sixty-nine <i>O</i>-glycopeptides of the long <i>O</i>-glycopeptides in tryptic bovine fetuin digest were obtained with a relative abundance range from 100 to 0.2%, which included sialylated glycans with Neu5Ac and Neu5Gc.
36074808	2	67	theme	liquid	380:385	arg1	spectrometry					414:425	liquid chromatography-tandem mass spectrometry	380:425	liquid chromatography-tandem mass spectrometry	380:425	We detected a wide range of abundances of various <i>O</i>-glycoforms in isomeric glycopeptides using hot electron capture dissociation (hot ECD) in liquid chromatography-tandem mass spectrometry.
36074808	0	68	theme	Capture	101:107	arg1	Dissociation					109:120	Hot Electron Capture Dissociation	88:120	Hot Electron Capture Dissociation	88:120	Localization of Multiple <i>O</i>-Linked Glycans Exhibited in Isomeric Glycopeptides by Hot Electron Capture Dissociation.
36140930	4	0	theme	polyphenol-polysaccharide	929:953	arg1	PPC					967:969	PPC	967:969	PPC	967:969	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	4	0	theme	polyphenol-polysaccharide	929:953	arg1	conjugates					955:964	two polyphenol-polysaccharide conjugates	925:964	two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa	925:1106	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	7	1	theme	fruit	1877:1881	arg1	cake					1889:1892	V. vitis-idaea fruit press cake	1862:1892	V. vitis-idaea fruit press cake	1862:1892	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	2	theme	V.	1862:1863	arg1	cake					1889:1892	V. vitis-idaea fruit press cake	1862:1892	V. vitis-idaea fruit press cake	1862:1892	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	3	3	theme	water-soluble	546:558	arg1	polysaccharides					560:574	water-soluble polysaccharides	546:574	water-soluble polysaccharides of V. vitis-idaea press cake	546:603	In this study, water-soluble polysaccharides of V. vitis-idaea press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography.
36140930	6	4	theme	cholesterol	1592:1602	arg1	weight					1569:1574	body weight	1564:1574	body weight	1564:1574	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	4	theme	cholesterol	1592:1602	arg1	level					1583:1587	serum level	1577:1587	serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde	1577:1680	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	5	5	theme	PPC	1282:1284	arg1	activity					1270:1277	expressed antioxidant activity	1248:1277	expressed antioxidant activity of PPC	1248:1284	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	1	6	theme	Northern	303:310	arg1	Hemisphere					312:321	the Northern Hemisphere	299:321	the Northern Hemisphere	299:321	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	3	7	theme	vitis-idaea	582:592	arg1	cake					600:603	V. vitis-idaea press cake	579:603	V. vitis-idaea press cake	579:603	In this study, water-soluble polysaccharides of V. vitis-idaea press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography.
36140930	1	8	dep	Vaccinium	201:209	arg1	L.					223:224	Vaccinium vitis-idaea L.	201:224	Vaccinium vitis-idaea L.	201:224	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	0	9	theme	Vaccinium	154:162	arg1	Cake					182:185	Vaccinium vitis-idaea Press Cake	154:185	Vaccinium vitis-idaea Press Cake	154:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	6	10	theme	body	1564:1567	arg1	weight					1569:1574	body weight	1564:1574	body weight	1564:1574	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	10	theme	body	1564:1567	arg1	level					1583:1587	serum level	1577:1587	serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde	1577:1680	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	1	11	theme	important	238:246	arg1	fruits					227:232	Lingonberry (Vaccinium vitis-idaea L.) fruits	188:232	Lingonberry (Vaccinium vitis-idaea L.) fruits	188:232	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	1	11	theme	important	238:246	arg1	berries					259:265	important Ericaceous berries	238:265	important Ericaceous berries	238:265	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	4	12	theme	monomeric	1018:1026	arg1	residues					1057:1064	monomeric and dimeric hydroxycinnamate residues	1018:1064	monomeric and dimeric hydroxycinnamate residues	1018:1064	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	6	13	theme	catalase	1772:1779	arg1	activity					1710:1717	the enzymatic activity	1696:1717	the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet	1696:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	0	14	theme	Press	176:180	arg1	Cake					182:185	Vaccinium vitis-idaea Press Cake	154:185	Vaccinium vitis-idaea Press Cake	154:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	6	15	theme	malondialdehyde	1666:1680	arg1	weight					1569:1574	body weight	1564:1574	body weight	1564:1574	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	15	theme	malondialdehyde	1666:1680	arg1	level					1583:1587	serum level	1577:1587	serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde	1577:1680	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	5	16	theme	antioxidant	1258:1268	arg1	activity					1270:1277	expressed antioxidant activity	1248:1277	expressed antioxidant activity of PPC	1248:1284	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	4	17	theme	monosaccharide	733:746	arg1	analysis					748:755	monosaccharide analysis	733:755	monosaccharide analysis	733:755	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	3	18	theme	cake	600:603	arg1	polysaccharides					560:574	water-soluble polysaccharides	546:574	water-soluble polysaccharides of V. vitis-idaea press cake	546:603	In this study, water-soluble polysaccharides of V. vitis-idaea press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography.
36140930	4	19	theme	neutral	975:981	arg1	arabinogalactans					983:998	neutral arabinogalactans	975:998	neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa	975:1106	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	6	20	theme	glutathione	1744:1754	arg1	peroxidase					1756:1765	glutathione peroxidase	1744:1765	glutathione peroxidase	1744:1765	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	21	theme	low/high-density	1620:1635	arg1	lipoprotein-cholesterol					1637:1659	low/high-density lipoprotein-cholesterol	1620:1659	low/high-density lipoprotein-cholesterol	1620:1659	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	4	22	theme	molecular	1071:1079	arg1	weights					1081:1087	molecular weights	1071:1087	molecular weights of 108 and 157 kDa	1071:1106	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	6	23	theme	dismutase	1733:1741	arg1	activity					1710:1717	the enzymatic activity	1696:1717	the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet	1696:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	5	24	theme	chelate	1370:1376	arg1	ions					1386:1389	chelate ferrous ions	1370:1389	chelate ferrous ions	1370:1389	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	0	25	theme	Antioxidant	62:72	arg1	Conjugates					138:147	Polyphenol-Polysaccharide Conjugates	112:147	Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake	112:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	0	25	theme	Antioxidant	62:72	arg1	Characterization					42:57	Characterization	42:57	Characterization of Antioxidant and Hypolipidemic Polysaccharides	42:106	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	5	26	theme	in	1205:1206	arg1	assays					1226:1231	in vitro and in vivo assays	1205:1231	in vitro and in vivo assays	1205:1231	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	6	27	theme	triglycerides	1605:1617	arg1	weight					1569:1574	body weight	1564:1574	body weight	1564:1574	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	27	theme	triglycerides	1605:1617	arg1	level					1583:1587	serum level	1577:1587	serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde	1577:1680	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	1	28	theme	Lingonberry	188:198	arg1	berries					259:265	important Ericaceous berries	238:265	important Ericaceous berries	238:265	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	1	28	theme	Lingonberry	188:198	arg1	fruits					227:232	Lingonberry (Vaccinium vitis-idaea L.) fruits	188:232	Lingonberry (Vaccinium vitis-idaea L.) fruits	188:232	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	1	28	theme	Lingonberry	188:198	arg1	Vaccinium					201:209	Vaccinium	201:209	Vaccinium	201:209	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	6	29	theme	1	1814:1814	arg1	%					1815:1815	%	1815:1815	%	1815:1815	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	4	30	theme	composition	720:730	arg1	results					699:705	The results	695:705	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction	695:896	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	0	31	theme	Hypolipidemic	78:90	arg1	Polysaccharides					92:106	Hypolipidemic Polysaccharides	78:106	Hypolipidemic Polysaccharides	78:106	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	6	32	theme	superoxide	1722:1731	arg1	dismutase					1733:1741	superoxide dismutase	1722:1741	superoxide dismutase	1722:1741	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	7	33	theme	cake	1889:1892	arg1	PPC					1855:1857	PPC	1855:1857	PPC	1855:1857	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	33	theme	cake	1889:1892	arg1	Polysaccharides					1835:1849	Polysaccharides	1835:1849	Polysaccharides	1835:1849	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	33	theme	cake	1889:1892	arg1	antioxidants					1917:1928	new antioxidants	1913:1928	new antioxidants	1913:1928	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	33	theme	cake	1889:1892	arg1	agents					1948:1953	hypolipidemic agents	1934:1953	hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders	1934:2025	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	4	34	theme	dimeric	1032:1038	arg1	residues					1057:1064	monomeric and dimeric hydroxycinnamate residues	1018:1064	monomeric and dimeric hydroxycinnamate residues	1018:1064	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	5	35	theme	hydrogen	1347:1354	arg1	peroxide					1356:1363	hydrogen peroxide	1347:1363	hydrogen peroxide	1347:1363	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	6	36	theme	PPC	1445:1447	arg1	potential					1427:1435	marked hypolipidemic potential	1406:1435	marked hypolipidemic potential of both PPC and acidic polymers	1406:1467	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	7	37	theme	metabolic	2007:2015	arg1	disorders					2017:2025	hyperlipidemic metabolic disorders	1992:2025	hyperlipidemic metabolic disorders	1992:2025	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	4	38	theme	weight	833:838	arg1	determination					840:852	molecular weight determination	823:852	molecular weight determination	823:852	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	2	39	theme	hard-skinned	424:435	arg1	waste					363:367	The waste	359:367	The waste generated by the V. vitis-idaea processing industry	359:419	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	2	39	theme	hard-skinned	424:435	arg1	cake					443:446	hard-skinned press cake	424:446	hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far	424:528	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	2	39	theme	hard-skinned	424:435	arg1	source					472:477	a potential source	460:477	a potential source of dietary fiber	460:494	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	0	40	theme	Polymeric	0:8	arg1	Compounds					10:18	Polymeric Compounds	0:18	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.	0:186	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	5	41	theme	expressed	1248:1256	arg1	activity					1270:1277	expressed antioxidant activity	1248:1277	expressed antioxidant activity of PPC	1248:1284	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	5	42	dep	in	1218:1219	arg1	vivo					1221:1224	vivo	1221:1224	vivo	1221:1224	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	4	43	theme	infrared	800:807	arg1	spectroscopy					809:820	ultraviolet-visible and Fourier-transform infrared spectroscopy	758:820	ultraviolet-visible and Fourier-transform infrared spectroscopy	758:820	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	0	44	theme	Waste	35:39	arg1	Compounds					10:18	Polymeric Compounds	0:18	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.	0:186	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	4	45	theme	kDa	1104:1106	arg1	weights					1081:1087	molecular weights	1071:1087	molecular weights of 108 and 157 kDa	1071:1106	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	4	46	theme	destruction	886:896	arg1	results					699:705	The results	695:705	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction	695:896	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	1	47	theme	bioactive	338:346	arg1	phenolics					348:356	bioactive phenolics	338:356	bioactive phenolics	338:356	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	7	48	theme	hypolipidemic	1934:1946	arg1	PPC					1855:1857	PPC	1855:1857	PPC	1855:1857	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	48	theme	hypolipidemic	1934:1946	arg1	Polysaccharides					1835:1849	Polysaccharides	1835:1849	Polysaccharides	1835:1849	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	48	theme	hypolipidemic	1934:1946	arg1	agents					1948:1953	hypolipidemic agents	1934:1953	hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders	1934:2025	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	6	49	theme	cholesterol	1817:1827	arg1	diet					1829:1832	a 1% cholesterol diet	1812:1832	a 1% cholesterol diet	1812:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	5	50	theme	phenolic-scavenged	1297:1314	arg1	radicals					1321:1328	phenolic-scavenged free radicals	1297:1328	phenolic-scavenged free radicals	1297:1328	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	7	51	theme	press	1883:1887	arg1	cake					1889:1892	V. vitis-idaea fruit press cake	1862:1892	V. vitis-idaea fruit press cake	1862:1892	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	4	52	theme	kDa	1183:1185	arg1	weights					1160:1166	molecular weights	1150:1166	molecular weights of 258 and 318 kDa	1150:1185	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	4	53	theme	analysis	863:870	arg1	results					699:705	The results	695:705	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction	695:896	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	2	54	theme	vitis-idaea	389:399	arg1	industry					412:419	the V. vitis-idaea processing industry	382:419	the V. vitis-idaea processing industry	382:419	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	6	55	theme	in	1541:1542	arg1	study					1550:1554	the in vitro study	1537:1554	the in vitro study	1537:1554	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	2	56	theme	dietary	482:488	arg1	fiber					490:494	dietary fiber	482:494	dietary fiber	482:494	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	7	57	theme	vitis-idaea	1865:1875	arg1	cake					1889:1892	V. vitis-idaea fruit press cake	1862:1892	V. vitis-idaea fruit press cake	1862:1892	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	4	58	theme	molecular	1150:1158	arg1	weights					1160:1166	molecular weights	1150:1166	molecular weights of 258 and 318 kDa	1150:1185	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	6	59	theme	polymers	1460:1467	arg1	potential					1427:1435	marked hypolipidemic potential	1406:1435	marked hypolipidemic potential of both PPC and acidic polymers	1406:1467	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	1	60	theme	healthy	283:289	arg1	diet					291:294	a healthy diet	281:294	a healthy diet of the Northern Hemisphere	281:321	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	4	61	with	galacturonans	1131:1143	arg1	weights					1160:1166	molecular weights	1150:1166	molecular weights of 258 and 318 kDa	1150:1185	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	6	62	theme	pancreatic	1516:1525	arg1	lipase					1527:1532	pancreatic lipase	1516:1532	pancreatic lipase in the in vitro study	1516:1554	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	63	with	hamsters	1798:1805	arg1	diet					1829:1832	a 1% cholesterol diet	1812:1832	a 1% cholesterol diet	1812:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	64	from	activity	1710:1717	arg1	livers					1788:1793	the livers	1784:1793	the livers of hamsters with a 1% cholesterol diet	1784:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	65	theme	acidic	1453:1458	arg1	polymers					1460:1467	acidic polymers	1453:1467	acidic polymers	1453:1467	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	3	66	theme	V.	579:580	arg1	cake					600:603	V. vitis-idaea press cake	579:603	V. vitis-idaea press cake	579:603	In this study, water-soluble polysaccharides of V. vitis-idaea press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography.
36140930	6	67	theme	serum	1577:1581	arg1	weight					1569:1574	body weight	1564:1574	body weight	1564:1574	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	67	theme	serum	1577:1581	arg1	level					1583:1587	serum level	1577:1587	serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde	1577:1680	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	0	68	theme	vitis-idaea	164:174	arg1	Cake					182:185	Vaccinium vitis-idaea Press Cake	154:185	Vaccinium vitis-idaea Press Cake	154:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	6	69	theme	marked	1406:1411	arg1	potential					1427:1435	marked hypolipidemic potential	1406:1435	marked hypolipidemic potential of both PPC and acidic polymers	1406:1467	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	1	70	theme	Ericaceous	248:257	arg1	fruits					227:232	Lingonberry (Vaccinium vitis-idaea L.) fruits	188:232	Lingonberry (Vaccinium vitis-idaea L.) fruits	188:232	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	1	70	theme	Ericaceous	248:257	arg1	berries					259:265	important Ericaceous berries	238:265	important Ericaceous berries	238:265	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	7	71	used	used	1979:1982	arg2	PPC					1855:1857	PPC	1855:1857	PPC	1855:1857	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	71	used	used	1979:1982	arg2	Polysaccharides					1835:1849	Polysaccharides	1835:1849	Polysaccharides	1835:1849	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	71	used	used	1979:1982	arg2	agents					1948:1953	hypolipidemic agents	1934:1953	hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders	1934:2025	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	3	72	theme	press	594:598	arg1	cake					600:603	V. vitis-idaea press cake	579:603	V. vitis-idaea press cake	579:603	In this study, water-soluble polysaccharides of V. vitis-idaea press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography.
36140930	6	73	theme	peroxidase	1756:1765	arg1	activity					1710:1717	the enzymatic activity	1696:1717	the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet	1696:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	74	theme	lipoprotein-cholesterol	1637:1659	arg1	weight					1569:1574	body weight	1564:1574	body weight	1564:1574	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	74	theme	lipoprotein-cholesterol	1637:1659	arg1	level					1583:1587	serum level	1577:1587	serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde	1577:1680	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	4	75	with	conjugates	955:964	arg1	weights					1160:1166	molecular weights	1150:1166	molecular weights of 258 and 318 kDa	1150:1185	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	4	76	theme	analysis	748:755	arg1	results					699:705	The results	695:705	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction	695:896	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	6	77	theme	bile	1474:1477	arg1	acids					1479:1483	bile acids	1474:1483	bile acids	1474:1483	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	6	78	theme	%	1815:1815	arg1	diet					1829:1832	a 1% cholesterol diet	1812:1832	a 1% cholesterol diet	1812:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	5	79	theme	nitrogen	1331:1338	arg1	oxide					1340:1344	nitrogen oxide	1331:1344	nitrogen oxide	1331:1344	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	0	80	theme	Polysaccharides	92:106	arg1	Conjugates					138:147	Polyphenol-Polysaccharide Conjugates	112:147	Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake	112:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	0	80	theme	Polysaccharides	92:106	arg1	Characterization					42:57	Characterization	42:57	Characterization of Antioxidant and Hypolipidemic Polysaccharides	42:106	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	6	81	theme	hypolipidemic	1413:1425	arg1	potential					1427:1435	marked hypolipidemic potential	1406:1435	marked hypolipidemic potential of both PPC and acidic polymers	1406:1467	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	4	82	theme	elemental	710:718	arg1	composition					720:730	elemental composition	710:730	elemental composition	710:730	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	0	83	theme	Polyphenol-Polysaccharide	112:136	arg1	Conjugates					138:147	Polyphenol-Polysaccharide Conjugates	112:147	Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake	112:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	5	84	theme	in	1218:1219	arg1	assays					1226:1231	in vitro and in vivo assays	1205:1231	in vitro and in vivo assays	1205:1231	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	7	85	theme	hyperlipidemic	1992:2005	arg1	disorders					2017:2025	hyperlipidemic metabolic disorders	1992:2025	hyperlipidemic metabolic disorders	1992:2025	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	6	86	theme	hamsters	1798:1805	arg1	livers					1788:1793	the livers	1784:1793	the livers of hamsters with a 1% cholesterol diet	1784:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	4	87	theme	spectroscopy	809:820	arg1	results					699:705	The results	695:705	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction	695:896	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	6	88	theme	enzymatic	1700:1708	arg1	activity					1710:1717	the enzymatic activity	1696:1717	the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet	1696:1832	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	4	89	theme	hydroxycinnamate	1040:1055	arg1	residues					1057:1064	monomeric and dimeric hydroxycinnamate residues	1018:1064	monomeric and dimeric hydroxycinnamate residues	1018:1064	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	4	90	theme	molecular	823:831	arg1	determination					840:852	molecular weight determination	823:852	molecular weight determination	823:852	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	2	91	theme	potential	462:470	arg1	cake					443:446	hard-skinned press cake	424:446	hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far	424:528	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	2	91	theme	potential	462:470	arg1	source					472:477	a potential source	460:477	a potential source of dietary fiber	460:494	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	4	92	theme	ultraviolet-visible	758:776	arg1	spectroscopy					809:820	ultraviolet-visible and Fourier-transform infrared spectroscopy	758:820	ultraviolet-visible and Fourier-transform infrared spectroscopy	758:820	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	5	93	theme	ferrous	1378:1384	arg1	ions					1386:1389	chelate ferrous ions	1370:1389	chelate ferrous ions	1370:1389	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	2	94	theme	processing	401:410	arg1	industry					412:419	the V. vitis-idaea processing industry	382:419	the V. vitis-idaea processing industry	382:419	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	0	95	theme	Lingonberry	23:33	arg1	Waste					35:39	Lingonberry Waste	23:39	Lingonberry Waste	23:39	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	1	96	theme	Hemisphere	312:321	arg1	diet					291:294	a healthy diet	281:294	a healthy diet of the Northern Hemisphere	281:321	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	4	97	theme	non-esterified	1116:1129	arg1	galacturonans					1131:1143	two non-esterified galacturonans	1112:1143	two non-esterified galacturonans with molecular weights of 258 and 318 kDa	1112:1185	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	5	98	dep	in	1205:1206	arg1	vitro					1208:1212	vitro	1208:1212	vitro	1208:1212	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	3	99	theme	ion-exchange	647:658	arg1	chromatography					679:692	ion-exchange and size-exclusion chromatography	647:692	chromatography	679:692	In this study, water-soluble polysaccharides of V. vitis-idaea press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography.
36140930	4	100	theme	Fourier-transform	782:798	arg1	spectroscopy					809:820	ultraviolet-visible and Fourier-transform infrared spectroscopy	758:820	ultraviolet-visible and Fourier-transform infrared spectroscopy	758:820	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	2	101	theme	fiber	490:494	arg1	cake					443:446	hard-skinned press cake	424:446	hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far	424:528	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	2	101	theme	fiber	490:494	arg1	source					472:477	a potential source	460:477	a potential source of dietary fiber	460:494	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	2	102	theme	press	437:441	arg1	waste					363:367	The waste	359:367	The waste generated by the V. vitis-idaea processing industry	359:419	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	2	102	theme	press	437:441	arg1	cake					443:446	hard-skinned press cake	424:446	hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far	424:528	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	2	102	theme	press	437:441	arg1	source					472:477	a potential source	460:477	a potential source of dietary fiber	460:494	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36140930	5	103	theme	assays	1226:1231	arg1	combination					1190:1200	A combination	1188:1200	A combination of in vitro and in vivo assays	1188:1231	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	4	104	theme	alkaline	877:884	arg1	destruction					886:896	alkaline destruction	877:896	alkaline destruction	877:896	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	1	105	theme	phenolics	348:356	arg1	source					328:333	a source	326:333	a source of bioactive phenolics	326:356	Lingonberry (Vaccinium vitis-idaea L.) fruits are important Ericaceous berries to include in a healthy diet of the Northern Hemisphere as a source of bioactive phenolics.
36140930	7	106	theme	new	1913:1915	arg1	PPC					1855:1857	PPC	1855:1857	PPC	1855:1857	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	106	theme	new	1913:1915	arg1	Polysaccharides					1835:1849	Polysaccharides	1835:1849	Polysaccharides	1835:1849	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	7	106	theme	new	1913:1915	arg1	antioxidants					1917:1928	new antioxidants	1913:1928	new antioxidants	1913:1928	Polysaccharides and PPC of V. vitis-idaea fruit press cake can be regarded as new antioxidants and hypolipidemic agents that can be potentially used to cure hyperlipidemic metabolic disorders.
36140930	6	107	from	lipase	1527:1532	arg1	study					1550:1554	the in vitro study	1537:1554	the in vitro study	1537:1554	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	0	108	from	Cake	182:185	arg1	Conjugates					138:147	Polyphenol-Polysaccharide Conjugates	112:147	Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake	112:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	0	108	from	Cake	182:185	arg1	Characterization					42:57	Characterization	42:57	Characterization of Antioxidant and Hypolipidemic Polysaccharides	42:106	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	3	109	theme	size-exclusion	664:677	arg1	chromatography					679:692	ion-exchange and size-exclusion chromatography	647:692	chromatography	679:692	In this study, water-soluble polysaccharides of V. vitis-idaea press cake were isolated, separated, and purified by ion-exchange and size-exclusion chromatography.
36140930	4	110	theme	determination	840:852	arg1	results					699:705	The results	695:705	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction	695:896	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	5	111	theme	free	1316:1319	arg1	radicals					1321:1328	phenolic-scavenged free radicals	1297:1328	phenolic-scavenged free radicals	1297:1328	A combination of in vitro and in vivo assays confirmed that expressed antioxidant activity of PPC was due to phenolic-scavenged free radicals, nitrogen oxide, hydrogen peroxide, and chelate ferrous ions.
36140930	0	112	dep	Compounds	10:18	arg1	Conjugates					138:147	Polyphenol-Polysaccharide Conjugates	112:147	Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake	112:185	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	0	112	dep	Compounds	10:18	arg1	Characterization					42:57	Characterization	42:57	Characterization of Antioxidant and Hypolipidemic Polysaccharides	42:106	Polymeric Compounds of Lingonberry Waste: Characterization of Antioxidant and Hypolipidemic Polysaccharides and Polyphenol-Polysaccharide Conjugates from Vaccinium vitis-idaea Press Cake.
36140930	6	113	dep	in	1541:1542	arg1	vitro					1544:1548	vitro	1544:1548	vitro	1544:1548	Additionally, marked hypolipidemic potential of both PPC and acidic polymers bind bile acids, cholesterol, and fat, inhibit pancreatic lipase in the in vitro study, reduce body weight, serum level of cholesterol, triglycerides, low/high-density lipoprotein-cholesterol, and malondialdehyde, and increase the enzymatic activity of superoxide dismutase, glutathione peroxidase, and catalase in the livers of hamsters with a 1% cholesterol diet.
36140930	4	114	theme	linkage	855:861	arg1	analysis					863:870	linkage analysis	855:870	linkage analysis	855:870	The results of elemental composition, monosaccharide analysis, ultraviolet-visible and Fourier-transform infrared spectroscopy, molecular weight determination, linkage analysis, and alkaline destruction allowed us to characterize two polyphenol-polysaccharide conjugates (PPC) as neutral arabinogalactans cross-linked with monomeric and dimeric hydroxycinnamate residues with molecular weights of 108 and 157 kDa and two non-esterified galacturonans with molecular weights of 258 and 318 kDa.
36140930	2	115	theme	V.	386:387	arg1	industry					412:419	the V. vitis-idaea processing industry	382:419	the V. vitis-idaea processing industry	382:419	The waste generated by the V. vitis-idaea processing industry is hard-skinned press cake that can be a potential source of dietary fiber and has not been studied thus far.
36824172	12	0	theme	UC	1765:1766	arg1	mice					1768:1771	UC mice	1765:1771	UC mice	1765:1771	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	16	1	theme	promising	2348:2356	arg1	candidate					2358:2366	a promising candidate	2346:2366	a promising candidate for the treatment of UC	2346:2390	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	16	1	theme	promising	2348:2356	arg1	inflammation					2330:2341	OXZ-induced intestinal inflammation	2307:2341	OXZ-induced intestinal inflammation	2307:2341	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	14	2	theme	polysaccharide	1916:1929	arg1	composition					1931:1941	The polysaccharide composition	1912:1941	The polysaccharide composition (molar ratio)	1912:1955	The polysaccharide composition (molar ratio) was composed of fucose (0.025), glucosamine hydrochloride (0.004), galactose (0.063), glucose (0.869), and mannose (0.038).
36824172	14	2	theme	polysaccharide	1916:1929	arg1	ratio					1950:1954	molar ratio	1944:1954	molar ratio	1944:1954	The polysaccharide composition (molar ratio) was composed of fucose (0.025), glucosamine hydrochloride (0.004), galactose (0.063), glucose (0.869), and mannose (0.038).
36824172	9	3	used	used	1255:1258	arg2	weight					1242:1247	80, 160, and 320 mg/kg body weight	1214:1247	80, 160, and 320 mg/kg body weight	1214:1247	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	3	used	used	1255:1258	arg2	doses					1200:1204	Different doses	1190:1204	Different doses of GFP (80, 160, and 320 mg/kg body weight)	1190:1248	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	12	4	dep	cytokines	1652:1660	arg1	TNF					1686:1688	TNF	1686:1688	TNF	1686:1688	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	4	dep	cytokines	1652:1660	arg1	cytokines					1652:1660	pro-inflammatory cytokines	1635:1660	pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β]	1635:1717	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	4	dep	cytokines	1652:1660	arg1	IL					1710:1711	interleukin (IL)-1	1697:1714	interleukin (IL)-1	1697:1714	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	4	dep	cytokines	1652:1660	arg1	factor					1678:1683	[tumor necrosis factor (TNF)-α	1662:1691	[tumor necrosis factor (TNF)-α	1662:1691	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	7	5	theme	red	1045:1047	arg1	testing					1049:1055	Congo red testing	1039:1055	Congo red testing	1039:1055	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	8	6	from	colitis	1135:1141	arg1	mice					1151:1154	mice	1151:1154	mice	1151:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	5	7	theme	extraction	513:522	arg1	conditions					494:503	The optimum extraction conditions	471:503	The optimum extraction conditions	471:503	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	5	7	theme	extraction	513:522	arg1	time					524:527	an extraction time	510:527	an extraction time of 71 min, an extraction temperature of 90°C	510:572	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	6	8	dep	transform	767:775	arg1	infrared					777:784	infrared	777:784	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry	767:896	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	11	9	theme	UC	1579:1580	arg1	mice					1582:1585	UC mice	1579:1585	UC mice	1579:1585	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	5	10	theme	ultrasonic	622:631	arg1	power					633:637	an ultrasonic power	619:637	an ultrasonic power of 500 W. GFP	619:651	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	11	11	theme	neutrophils	1564:1574	arg1	decreases					1493:1501	decreases	1493:1501	decreases in colon length	1493:1517	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	11	11	theme	neutrophils	1564:1574	arg1	levels					1527:1532	the levels	1523:1532	the levels of leukocytes, platelets, and neutrophils in UC mice	1523:1585	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	13	12	theme	polysaccharide	1836:1849	arg1	yield					1851:1855	the predicted polysaccharide yield	1822:1855	the predicted polysaccharide yield	1822:1855	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	13	12	theme	polysaccharide	1836:1849	arg1	%					1866:1866	21.72%	1861:1866	21.72%	1861:1866	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	5	13	theme	extraction	483:492	arg1	conditions					494:503	The optimum extraction conditions	471:503	The optimum extraction conditions	471:503	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	5	13	theme	extraction	483:492	arg1	time					524:527	an extraction time	510:527	an extraction time of 71 min, an extraction temperature of 90°C	510:572	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	8	14	theme	ulcerative	1124:1133	arg1	UC					1144:1145	UC	1144:1145	UC	1144:1145	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	8	14	theme	ulcerative	1124:1133	arg1	colitis					1135:1141	oxazolone (OXZ)-induced ulcerative colitis	1100:1141	oxazolone (OXZ)-induced ulcerative colitis (UC) in mice	1100:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	0	15	from	mushrooms	103:111	arg1	properties					11:20	Structural properties	0:20	Structural properties	0:20	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	0	15	from	mushrooms	103:111	arg1	activity					44:51	anti-inflammatory activity	26:51	anti-inflammatory activity	26:51	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	0	15	from	mushrooms	103:111	arg1	polysaccharides					65:79	purified polysaccharides	56:79	purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa)	56:130	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	10	16	theme	blood	1421:1425	arg1	samples					1444:1450	blood and colon tissue samples	1421:1450	blood and colon tissue samples	1421:1450	After treatment, the mice were killed, and blood and colon tissue samples were collected.
36824172	7	17	theme	scanning	1004:1011	arg1	TG-DSC					1026:1031	TG-DSC	1026:1031	TG-DSC	1026:1031	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	7	17	theme	scanning	1004:1011	arg1	calorimetry					1013:1023	thermogravimetric differential scanning calorimetry	973:1023	thermogravimetric differential scanning calorimetry (TG-DSC)	973:1032	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	6	18	theme	visible	879:885	arg1	photometry					887:896	ultraviolet (UV) visible photometry	862:896	ultraviolet (UV) visible photometry	862:896	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	7	19	theme	thermogravimetric	973:989	arg1	TG-DSC					1026:1031	TG-DSC	1026:1031	TG-DSC	1026:1031	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	7	19	theme	thermogravimetric	973:989	arg1	calorimetry					1013:1023	thermogravimetric differential scanning calorimetry	973:1023	thermogravimetric differential scanning calorimetry (TG-DSC)	973:1032	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	7	20	theme	scanning	937:944	arg1	SEM					967:969	SEM	967:969	SEM	967:969	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	7	20	theme	scanning	937:944	arg1	microscopy					955:964	scanning electron microscopy	937:964	scanning electron microscopy (SEM)	937:970	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	9	21	theme	body	1328:1331	arg1	weight					1333:1338	370 mg/kg body weight	1318:1338	370 mg/kg body weight	1318:1338	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	21	theme	body	1328:1331	arg1	control					1309:1315	a positive control	1298:1315	a positive control (370 mg/kg body weight)	1298:1339	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	2	22	theme	main	245:248	arg1	polysaccharides					290:304	polysaccharides	290:304	polysaccharides	290:304	The main active ingredients of this mushroom are polysaccharides.
36824172	2	22	theme	main	245:248	arg1	ingredients					257:267	The main active ingredients	241:267	The main active ingredients of this mushroom	241:284	The main active ingredients of this mushroom are polysaccharides.
36824172	7	23	theme	GFP	917:919	arg1	morphology					903:912	The morphology	899:912	The morphology of GFP	899:919	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	1	24	theme	medicinal	163:171	arg1	mushroom					173:180	an edible medicinal mushroom	153:180	an edible medicinal mushroom that has been proven to have a variety of health benefits	153:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	1	24	theme	medicinal	163:171	arg1	frondosa					141:148	Grifola frondosa	133:148	Grifola frondosa	133:148	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	14	25	theme	glucosamine	1989:1999	arg1	0.004					2016:2020	0.004	2016:2020	0.004	2016:2020	The polysaccharide composition (molar ratio) was composed of fucose (0.025), glucosamine hydrochloride (0.004), galactose (0.063), glucose (0.869), and mannose (0.038).
36824172	14	25	theme	glucosamine	1989:1999	arg1	hydrochloride					2001:2013	glucosamine hydrochloride	1989:2013	glucosamine hydrochloride (0.004)	1989:2021	The polysaccharide composition (molar ratio) was composed of fucose (0.025), glucosamine hydrochloride (0.004), galactose (0.063), glucose (0.869), and mannose (0.038).
36824172	5	26	theme	W.	646:647	arg1	GFP					649:651	500 W. GFP	642:651	500 W. GFP	642:651	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	9	27	theme	GFP	1209:1211	arg1	weight					1242:1247	80, 160, and 320 mg/kg body weight	1214:1247	80, 160, and 320 mg/kg body weight	1214:1247	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	27	theme	GFP	1209:1211	arg1	doses					1200:1204	Different doses	1190:1204	Different doses of GFP (80, 160, and 320 mg/kg body weight)	1190:1248	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	3	28	used	used	357:360	arg2	extraction					342:351	ultrasonic-assisted extraction	322:351	ultrasonic-assisted extraction	322:351	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	5	29	theme	90°C	569:572	arg1	min					535:537	71 min	532:537	71 min	532:537	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	5	29	theme	90°C	569:572	arg1	temperature					554:564	an extraction temperature	540:564	an extraction temperature of 90°C	540:572	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	9	30	theme	UC	1374:1375	arg1	treatment					1349:1357	the treatment	1345:1357	the treatment of OXZ-induced UC	1345:1375	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	0	31	from	activity	44:51	arg1	mushrooms					103:111	Hen-of-the-woods mushrooms	86:111	Hen-of-the-woods mushrooms (Grifola frondosa)	86:130	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	0	31	from	activity	44:51	arg1	frondosa					122:129	frondosa	122:129	frondosa	122:129	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	10	32	theme	tissue	1437:1442	arg1	samples					1444:1450	blood and colon tissue samples	1421:1450	blood and colon tissue samples	1421:1450	After treatment, the mice were killed, and blood and colon tissue samples were collected.
36824172	0	33	theme	Structural	0:9	arg1	properties					11:20	Structural properties	0:20	Structural properties	0:20	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	15	34	theme	absorption	2118:2127	arg1	peak					2129:2132	a typical absorption peak	2108:2132	a typical absorption peak	2108:2132	GFP was also found to have a typical absorption peak, and the GFP extracted using the ultrasound-assisted extraction protocol was mainly β-glucan.
36824172	9	35	used	used	1290:1293	arg2	control					1309:1315	a positive control	1298:1315	a positive control (370 mg/kg body weight)	1298:1339	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	35	used	used	1290:1293	arg2	SASP					1280:1283	SASP	1280:1283	SASP	1280:1283	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	35	used	used	1290:1293	arg2	sulfapyridine					1265:1277	sulfapyridine	1265:1277	sulfapyridine (SASP)	1265:1284	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	3	36	theme	Grifola	378:384	arg1	polysaccharides					395:409	crude Grifola frondosa polysaccharides	372:409	crude Grifola frondosa polysaccharides (GFPs)	372:416	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	3	36	theme	Grifola	378:384	arg1	GFPs					412:415	GFPs	412:415	GFPs	412:415	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	6	37	theme	ion	833:835	arg1	IC					853:854	IC	853:854	IC	853:854	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	6	37	theme	ion	833:835	arg1	chromatography					837:850	ion chromatography	833:850	ion chromatography (IC)	833:855	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	12	38	theme	cytokines	1652:1660	arg1	expression					1621:1630	the expression	1617:1630	the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β]	1617:1717	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	0	39	theme	purified	56:63	arg1	polysaccharides					65:79	purified polysaccharides	56:79	purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa)	56:130	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	9	40	theme	mg/kg	1231:1235	arg1	weight					1242:1247	80, 160, and 320 mg/kg body weight	1214:1247	80, 160, and 320 mg/kg body weight	1214:1247	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	40	theme	mg/kg	1231:1235	arg1	doses					1200:1204	Different doses	1190:1204	Different doses of GFP (80, 160, and 320 mg/kg body weight)	1190:1248	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	5	41	theme	min	535:537	arg1	conditions					494:503	The optimum extraction conditions	471:503	The optimum extraction conditions	471:503	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	5	41	theme	min	535:537	arg1	time					524:527	an extraction time	510:527	an extraction time of 71 min, an extraction temperature of 90°C	510:572	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	1	42	theme	health	224:229	arg1	benefits					231:238	health benefits	224:238	health benefits	224:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	6	43	theme	X-ray	808:812	arg1	diffraction					814:824	X-ray diffraction	808:824	X-ray diffraction (XRD)	808:830	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	6	43	theme	X-ray	808:812	arg1	XRD					827:829	XRD	827:829	XRD	827:829	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	5	44	from	time	524:527	arg1	ratio					595:599	a solid-to-liquid ratio	577:599	a solid-to-liquid ratio of 1:37 g/mL	577:612	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	16	45	theme	OXZ-induced	2307:2317	arg1	candidate					2358:2366	a promising candidate	2346:2366	a promising candidate for the treatment of UC	2346:2390	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	16	45	theme	OXZ-induced	2307:2317	arg1	inflammation					2330:2341	OXZ-induced intestinal inflammation	2307:2341	OXZ-induced intestinal inflammation	2307:2341	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	14	46	theme	molar	1944:1948	arg1	composition					1931:1941	The polysaccharide composition	1912:1941	The polysaccharide composition (molar ratio)	1912:1955	The polysaccharide composition (molar ratio) was composed of fucose (0.025), glucosamine hydrochloride (0.004), galactose (0.063), glucose (0.869), and mannose (0.038).
36824172	14	46	theme	molar	1944:1948	arg1	ratio					1950:1954	molar ratio	1944:1954	molar ratio	1944:1954	The polysaccharide composition (molar ratio) was composed of fucose (0.025), glucosamine hydrochloride (0.004), galactose (0.063), glucose (0.869), and mannose (0.038).
36824172	11	47	theme	colon	1506:1510	arg1	length					1512:1517	colon length	1506:1517	colon length	1506:1517	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	6	48	theme	structural	704:713	arg1	characterization					715:730	The structural characterization	700:730	The structural characterization of GFP	700:737	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	12	49	theme	colon	1749:1753	arg1	injury					1755:1760	colon injury	1749:1760	colon injury in UC mice	1749:1771	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	50	from	injury	1755:1760	arg1	mice					1768:1771	UC mice	1765:1771	UC mice	1765:1771	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	15	51	contain	have	2103:2106	arg1	GFP					2081:2083	GFP	2081:2083	GFP	2081:2083	GFP was also found to have a typical absorption peak, and the GFP extracted using the ultrasound-assisted extraction protocol was mainly β-glucan.
36824172	15	51	contain	have	2103:2106	arg2	peak					2129:2132	a typical absorption peak	2108:2132	a typical absorption peak	2108:2132	GFP was also found to have a typical absorption peak, and the GFP extracted using the ultrasound-assisted extraction protocol was mainly β-glucan.
36824172	7	52	theme	Congo	1039:1043	arg1	testing					1049:1055	Congo red testing	1039:1055	Congo red testing	1039:1055	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	15	53	theme	ultrasound-assisted	2167:2185	arg1	protocol					2198:2205	the ultrasound-assisted extraction protocol	2163:2205	the ultrasound-assisted extraction protocol	2163:2205	GFP was also found to have a typical absorption peak, and the GFP extracted using the ultrasound-assisted extraction protocol was mainly β-glucan.
36824172	6	54	dep	Fourier	759:765	arg1	transform					767:775	transform	767:775	transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry	767:896	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	2	55	theme	mushroom	277:284	arg1	polysaccharides					290:304	polysaccharides	290:304	polysaccharides	290:304	The main active ingredients of this mushroom are polysaccharides.
36824172	2	55	theme	mushroom	277:284	arg1	ingredients					257:267	The main active ingredients	241:267	The main active ingredients of this mushroom	241:284	The main active ingredients of this mushroom are polysaccharides.
36824172	8	56	theme	GFP	1093:1095	arg1	administration					1075:1088	the administration	1071:1088	the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice	1071:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	16	57	theme	intestinal	2460:2469	arg1	diseases					2471:2478	intestinal diseases	2460:2478	intestinal diseases	2460:2478	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	8	58	theme	oxazolone	1100:1108	arg1	UC					1144:1145	UC	1144:1145	UC	1144:1145	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	8	58	theme	oxazolone	1100:1108	arg1	colitis					1135:1141	oxazolone (OXZ)-induced ulcerative colitis	1100:1141	oxazolone (OXZ)-induced ulcerative colitis (UC) in mice	1100:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	12	59	dep	factor	1678:1683	arg1	β					1716:1716	β	1716:1716	β	1716:1716	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	5	60	theme	optimum	475:481	arg1	conditions					494:503	The optimum extraction conditions	471:503	The optimum extraction conditions	471:503	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	5	60	theme	optimum	475:481	arg1	time					524:527	an extraction time	510:527	an extraction time of 71 min, an extraction temperature of 90°C	510:572	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	8	61	theme	OXZ	1111:1113	arg1	UC					1144:1145	UC	1144:1145	UC	1144:1145	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	8	61	theme	OXZ	1111:1113	arg1	colitis					1135:1141	oxazolone (OXZ)-induced ulcerative colitis	1100:1141	oxazolone (OXZ)-induced ulcerative colitis (UC) in mice	1100:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	7	62	theme	differential	991:1002	arg1	TG-DSC					1026:1031	TG-DSC	1026:1031	TG-DSC	1026:1031	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	7	62	theme	differential	991:1002	arg1	calorimetry					1013:1023	thermogravimetric differential scanning calorimetry	973:1023	thermogravimetric differential scanning calorimetry (TG-DSC)	973:1032	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	9	63	theme	positive	1300:1307	arg1	sulfapyridine					1265:1277	sulfapyridine	1265:1277	sulfapyridine (SASP)	1265:1284	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	63	theme	positive	1300:1307	arg1	weight					1333:1338	370 mg/kg body weight	1318:1338	370 mg/kg body weight	1318:1338	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	63	theme	positive	1300:1307	arg1	control					1309:1315	a positive control	1298:1315	a positive control (370 mg/kg body weight)	1298:1339	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	8	64	theme	-induced	1115:1122	arg1	UC					1144:1145	UC	1144:1145	UC	1144:1145	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	8	64	theme	-induced	1115:1122	arg1	colitis					1135:1141	oxazolone (OXZ)-induced ulcerative colitis	1100:1141	oxazolone (OXZ)-induced ulcerative colitis (UC) in mice	1100:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	5	65	theme	GFP	649:651	arg1	power					633:637	an ultrasonic power	619:637	an ultrasonic power of 500 W. GFP	619:651	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	5	66	theme	extraction	543:552	arg1	min					535:537	71 min	532:537	71 min	532:537	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	5	66	theme	extraction	543:552	arg1	temperature					554:564	an extraction temperature	540:564	an extraction temperature of 90°C	540:572	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	1	67	theme	Grifola	133:139	arg1	mushroom					173:180	an edible medicinal mushroom	153:180	an edible medicinal mushroom that has been proven to have a variety of health benefits	153:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	1	67	theme	Grifola	133:139	arg1	frondosa					141:148	Grifola frondosa	133:148	Grifola frondosa	133:148	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	16	68	theme	GFP	2290:2292	arg1	extraction					2276:2285	ultrasound-assisted extraction	2256:2285	ultrasound-assisted extraction of GFP	2256:2292	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	11	69	from	levels	1527:1532	arg1	mice					1582:1585	UC mice	1579:1585	UC mice	1579:1585	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	11	69	from	levels	1527:1532	arg1	length					1512:1517	colon length	1506:1517	colon length	1506:1517	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	4	70	theme	purified	425:432	arg1	GFP					434:436	purified GFP	425:436	purified GFP	425:436	Then, purified GFP was obtained after purification.
36824172	8	71	theme	weight	1177:1182	arg1	loss					1184:1187	weight loss	1177:1187	weight loss	1177:1187	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	7	72	theme	electron	946:953	arg1	SEM					967:969	SEM	967:969	SEM	967:969	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	7	72	theme	electron	946:953	arg1	microscopy					955:964	scanning electron microscopy	937:964	scanning electron microscopy (SEM)	937:970	The morphology of GFP was analyzed by scanning electron microscopy (SEM), thermogravimetric differential scanning calorimetry (TG-DSC), and Congo red testing.
36824172	9	73	theme	mg/kg	1322:1326	arg1	weight					1333:1338	370 mg/kg body weight	1318:1338	370 mg/kg body weight	1318:1338	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	73	theme	mg/kg	1322:1326	arg1	control					1309:1315	a positive control	1298:1315	a positive control (370 mg/kg body weight)	1298:1339	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	11	74	theme	leukocytes	1537:1546	arg1	decreases					1493:1501	decreases	1493:1501	decreases in colon length	1493:1517	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	11	74	theme	leukocytes	1537:1546	arg1	levels					1527:1532	the levels	1523:1532	the levels of leukocytes, platelets, and neutrophils in UC mice	1523:1585	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	0	75	theme	Hen-of-the-woods	86:101	arg1	mushrooms					103:111	Hen-of-the-woods mushrooms	86:111	Hen-of-the-woods mushrooms (Grifola frondosa)	86:130	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	0	75	theme	Hen-of-the-woods	86:101	arg1	frondosa					122:129	frondosa	122:129	frondosa	122:129	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	13	76	theme	predicted	1826:1834	arg1	yield					1851:1855	the predicted polysaccharide yield	1822:1855	the predicted polysaccharide yield	1822:1855	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	13	76	theme	predicted	1826:1834	arg1	%					1866:1866	21.72%	1861:1866	21.72%	1861:1866	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	2	77	theme	active	250:255	arg1	polysaccharides					290:304	polysaccharides	290:304	polysaccharides	290:304	The main active ingredients of this mushroom are polysaccharides.
36824172	2	77	theme	active	250:255	arg1	ingredients					257:267	The main active ingredients	241:267	The main active ingredients of this mushroom	241:284	The main active ingredients of this mushroom are polysaccharides.
36824172	1	78	theme	edible	156:161	arg1	mushroom					173:180	an edible medicinal mushroom	153:180	an edible medicinal mushroom that has been proven to have a variety of health benefits	153:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	1	78	theme	edible	156:161	arg1	frondosa					141:148	Grifola frondosa	133:148	Grifola frondosa	133:148	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	16	79	theme	food	2433:2436	arg1	supplement					2438:2447	a food supplement	2431:2447	a food supplement to improve intestinal diseases	2431:2478	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	13	80	theme	actual	1877:1882	arg1	rate					1895:1898	the actual extraction rate	1873:1898	the actual extraction rate	1873:1898	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	13	80	theme	actual	1877:1882	arg1	%					1909:1909	21.13%	1904:1909	21.13%	1904:1909	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	3	81	theme	ultrasonic-assisted	322:340	arg1	extraction					342:351	ultrasonic-assisted extraction	322:351	ultrasonic-assisted extraction	322:351	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	0	82	from	properties	11:20	arg1	mushrooms					103:111	Hen-of-the-woods mushrooms	86:111	Hen-of-the-woods mushrooms (Grifola frondosa)	86:130	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	0	82	from	properties	11:20	arg1	frondosa					122:129	frondosa	122:129	frondosa	122:129	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	9	83	theme	Different	1190:1198	arg1	weight					1242:1247	80, 160, and 320 mg/kg body weight	1214:1247	80, 160, and 320 mg/kg body weight	1214:1247	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	83	theme	Different	1190:1198	arg1	doses					1200:1204	Different doses	1190:1204	Different doses of GFP (80, 160, and 320 mg/kg body weight)	1190:1248	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	12	84	theme	interleukin	1697:1707	arg1	cytokines					1652:1660	pro-inflammatory cytokines	1635:1660	pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β]	1635:1717	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	84	theme	interleukin	1697:1707	arg1	IL					1710:1711	interleukin (IL)-1	1697:1714	interleukin (IL)-1	1697:1714	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	3	85	theme	crude	372:376	arg1	polysaccharides					395:409	crude Grifola frondosa polysaccharides	372:409	crude Grifola frondosa polysaccharides (GFPs)	372:416	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	3	85	theme	crude	372:376	arg1	GFPs					412:415	GFPs	412:415	GFPs	412:415	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	11	86	theme	platelets	1549:1557	arg1	decreases					1493:1501	decreases	1493:1501	decreases in colon length	1493:1517	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	11	86	theme	platelets	1549:1557	arg1	levels					1527:1532	the levels	1523:1532	the levels of leukocytes, platelets, and neutrophils in UC mice	1523:1585	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	5	87	theme	g/mL	609:612	arg1	ratio					595:599	a solid-to-liquid ratio	577:599	a solid-to-liquid ratio of 1:37 g/mL	577:612	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	9	88	theme	OXZ-induced	1362:1372	arg1	UC					1374:1375	OXZ-induced UC	1362:1375	OXZ-induced UC	1362:1375	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	11	89	from	decreases	1493:1501	arg1	mice					1582:1585	UC mice	1579:1585	UC mice	1579:1585	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	11	89	from	decreases	1493:1501	arg1	length					1512:1517	colon length	1506:1517	colon length	1506:1517	GFP was found to prevent decreases in colon length and the levels of leukocytes, platelets, and neutrophils in UC mice.
36824172	13	90	theme	extraction	1884:1893	arg1	rate					1895:1898	the actual extraction rate	1873:1898	the actual extraction rate	1873:1898	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	13	90	theme	extraction	1884:1893	arg1	%					1909:1909	21.13%	1904:1909	21.13%	1904:1909	The results showed that Under these conditions, the predicted polysaccharide yield was 21.72%, and the actual extraction rate was 21.13%.
36824172	15	91	theme	typical	2110:2116	arg1	peak					2129:2132	a typical absorption peak	2108:2132	a typical absorption peak	2108:2132	GFP was also found to have a typical absorption peak, and the GFP extracted using the ultrasound-assisted extraction protocol was mainly β-glucan.
36824172	5	92	theme	Sephadex	684:691	arg1	G-100					693:697	Sephadex G-100	684:697	Sephadex G-100	684:697	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	1	93	contain	have	206:209	arg2	variety					213:219	a variety	211:219	a variety of health benefits	211:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	1	93	contain	have	206:209	arg1	mushroom					173:180	an edible medicinal mushroom	153:180	an edible medicinal mushroom that has been proven to have a variety of health benefits	153:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	1	93	contain	have	206:209	arg1	frondosa					141:148	Grifola frondosa	133:148	Grifola frondosa	133:148	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	1	93	contain	have	206:209	arg2	benefits					231:238	health benefits	224:238	health benefits	224:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	10	94	theme	colon	1431:1435	arg1	samples					1444:1450	blood and colon tissue samples	1421:1450	blood and colon tissue samples	1421:1450	After treatment, the mice were killed, and blood and colon tissue samples were collected.
36824172	0	95	theme	anti-inflammatory	26:42	arg1	activity					44:51	anti-inflammatory activity	26:51	anti-inflammatory activity	26:51	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	12	96	theme	pro-inflammatory	1635:1650	arg1	cytokines					1652:1660	pro-inflammatory cytokines	1635:1660	pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β]	1635:1717	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	96	theme	pro-inflammatory	1635:1650	arg1	IL					1710:1711	interleukin (IL)-1	1697:1714	interleukin (IL)-1	1697:1714	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	96	theme	pro-inflammatory	1635:1650	arg1	factor					1678:1683	[tumor necrosis factor (TNF)-α	1662:1691	[tumor necrosis factor (TNF)-α	1662:1691	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	5	97	theme	solid-to-liquid	579:593	arg1	ratio					595:599	a solid-to-liquid ratio	577:599	a solid-to-liquid ratio of 1:37 g/mL	577:612	The optimum extraction conditions were an extraction time of 71 min, an extraction temperature of 90°C in a solid-to-liquid ratio of 1:37 g/mL, and an ultrasonic power of 500 W. GFP was purified using DEAE-52 and Sephadex G-100.
36824172	16	98	theme	UC	2389:2390	arg1	treatment					2376:2384	the treatment	2372:2384	the treatment of UC	2372:2390	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	3	99	theme	frondosa	386:393	arg1	polysaccharides					395:409	crude Grifola frondosa polysaccharides	372:409	crude Grifola frondosa polysaccharides (GFPs)	372:416	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	3	99	theme	frondosa	386:393	arg1	GFPs					412:415	GFPs	412:415	GFPs	412:415	In this study, ultrasonic-assisted extraction was used to obtain crude Grifola frondosa polysaccharides (GFPs).
36824172	12	100	theme	[tumor	1662:1667	arg1	TNF					1686:1688	TNF	1686:1688	TNF	1686:1688	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	100	theme	[tumor	1662:1667	arg1	cytokines					1652:1660	pro-inflammatory cytokines	1635:1660	pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β]	1635:1717	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	100	theme	[tumor	1662:1667	arg1	factor					1678:1683	[tumor necrosis factor (TNF)-α	1662:1691	[tumor necrosis factor (TNF)-α	1662:1691	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	0	101	theme	polysaccharides	65:79	arg1	properties					11:20	Structural properties	0:20	Structural properties	0:20	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	0	101	theme	polysaccharides	65:79	arg1	activity					44:51	anti-inflammatory activity	26:51	anti-inflammatory activity	26:51	Structural properties and anti-inflammatory activity of purified polysaccharides from Hen-of-the-woods mushrooms (Grifola frondosa).
36824172	12	102	theme	necrosis	1669:1676	arg1	TNF					1686:1688	TNF	1686:1688	TNF	1686:1688	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	102	theme	necrosis	1669:1676	arg1	cytokines					1652:1660	pro-inflammatory cytokines	1635:1660	pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β]	1635:1717	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	12	102	theme	necrosis	1669:1676	arg1	factor					1678:1683	[tumor necrosis factor (TNF)-α	1662:1691	[tumor necrosis factor (TNF)-α	1662:1691	Moreover, GFP also decreased the expression of pro-inflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-1 β], increased IL-10, and reduced colon injury in UC mice.
36824172	6	103	theme	GFP	735:737	arg1	characterization					715:730	The structural characterization	700:730	The structural characterization of GFP	700:737	The structural characterization of GFP was performed using Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), ion chromatography (IC), and ultraviolet (UV) visible photometry.
36824172	8	104	located	found	1160:1164	arg1	addition					1061:1068	addition	1061:1068	addition	1061:1068	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	8	104	located	found	1160:1164	arg2	administration					1075:1088	the administration	1071:1088	the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice	1071:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	9	105	theme	body	1237:1240	arg1	weight					1242:1247	80, 160, and 320 mg/kg body weight	1214:1247	80, 160, and 320 mg/kg body weight	1214:1247	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	9	105	theme	body	1237:1240	arg1	doses					1200:1204	Different doses	1190:1204	Different doses of GFP (80, 160, and 320 mg/kg body weight)	1190:1248	Different doses of GFP (80, 160, and 320 mg/kg body weight) were used, and sulfapyridine (SASP) was used as a positive control (370 mg/kg body weight) for the treatment of OXZ-induced UC.
36824172	16	106	theme	intestinal	2319:2328	arg1	candidate					2358:2366	a promising candidate	2346:2366	a promising candidate for the treatment of UC	2346:2390	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	16	106	theme	intestinal	2319:2328	arg1	inflammation					2330:2341	OXZ-induced intestinal inflammation	2307:2341	OXZ-induced intestinal inflammation	2307:2341	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36824172	1	107	theme	benefits	231:238	arg1	variety					213:219	a variety	211:219	a variety of health benefits	211:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	1	107	theme	benefits	231:238	arg1	benefits					231:238	health benefits	224:238	health benefits	224:238	Grifola frondosa is an edible medicinal mushroom that has been proven to have a variety of health benefits.
36824172	8	108	from	administration	1075:1088	arg1	UC					1144:1145	UC	1144:1145	UC	1144:1145	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	8	108	from	administration	1075:1088	arg1	colitis					1135:1141	oxazolone (OXZ)-induced ulcerative colitis	1100:1141	oxazolone (OXZ)-induced ulcerative colitis (UC) in mice	1100:1154	In addition, the administration of GFP in oxazolone (OXZ)-induced ulcerative colitis (UC) in mice was found to prevent weight loss.
36824172	15	109	theme	extraction	2187:2196	arg1	protocol					2198:2205	the ultrasound-assisted extraction protocol	2163:2205	the ultrasound-assisted extraction protocol	2163:2205	GFP was also found to have a typical absorption peak, and the GFP extracted using the ultrasound-assisted extraction protocol was mainly β-glucan.
36824172	16	110	theme	ultrasound-assisted	2256:2274	arg1	extraction					2276:2285	ultrasound-assisted extraction	2256:2285	ultrasound-assisted extraction of GFP	2256:2292	These results indicate that ultrasound-assisted extraction of GFP could reduce OXZ-induced intestinal inflammation as a promising candidate for the treatment of UC, with the potential for development as a food supplement to improve intestinal diseases.
36204611	4	0	from	proteins	734:741	arg1	matrix					750:755	the matrix	746:755	the matrix	746:755	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	1	1	theme	human	147:151	arg1	hormones					153:160	human hormones	147:160	human hormones	147:160	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	1	2	theme	hormones	153:160	arg1	impact					137:142	the impact	133:142	the impact of human hormones on commensal microbiota and microbial biofilms	133:207	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	5	3	theme	incubation	922:931	arg1	h					917:917	24 h	914:917	24 h of incubation	914:931	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	0	4	theme	human	103:107	arg1	skin					109:112	human skin	103:112	human skin	103:112	Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin.
36204611	3	5	theme	M	494:494	arg1	×					487:487	concentration 4.9 ×	469:487	concentration 4.9 × 10-9 M which is close to normal blood plasma level	469:538	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	4	6	theme	abundant	725:732	arg1	proteins					734:741	the most abundant proteins	716:741	the most abundant proteins in the matrix	716:755	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	3	7	theme	plasma	527:532	arg1	level					534:538	normal blood plasma level	514:538	normal blood plasma level	514:538	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	7	8	theme	wide	1126:1129	arg1	perspectives					1131:1142	wide perspectives	1126:1142	wide perspectives for the future research	1126:1166	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	3	9	theme	normal	514:519	arg1	level					534:538	normal blood plasma level	514:538	normal blood plasma level	514:538	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	1	10	from	impact	137:142	arg1	microbiota					175:184	commensal microbiota	165:184	commensal microbiota	165:184	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	1	10	from	impact	137:142	arg1	biofilms					200:207	microbial biofilms	190:207	microbial biofilms	190:207	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	2	11	theme	skin	343:346	arg1	luteus					370:375	the skin commensal Micrococcus luteus C01	339:379	the skin commensal Micrococcus luteus C01	339:379	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	4	12	theme	Ef-Tu	692:696	arg1	proteins					734:741	the most abundant proteins	716:741	the most abundant proteins in the matrix	716:755	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	4	12	theme	Ef-Tu	692:696	arg1	one					709:711	one	709:711	one	709:711	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	4	12	theme	Ef-Tu	692:696	arg1	factor					698:703	The Ef-Tu factor	688:703	The Ef-Tu factor	688:703	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	0	13	from	composition	51:61	arg1	luteus					78:83	Micrococcus luteus	66:83	Micrococcus luteus C01 isolated from human skin	66:112	Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin.
36204611	2	14	from	effects	328:334	arg1	luteus					370:375	the skin commensal Micrococcus luteus C01	339:379	the skin commensal Micrococcus luteus C01	339:379	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	2	14	from	effects	328:334	arg1	biofilms					389:396	its biofilms	385:396	its biofilms	385:396	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	5	15	from	absent	860:865	arg1	presence					884:891	presence	884:891	presence of epinephrine	884:906	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	5	15	from	absent	860:865	arg1	matrix					874:879	the matrix	870:879	the matrix	870:879	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	7	16	theme	great	1037:1041	arg1	impact					1043:1048	a great impact	1035:1048	a great impact on the M. luteus biofilms and their matrix composition	1035:1103	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	3	17	from	Epinephrine	454:464	arg1	×					487:487	concentration 4.9 ×	469:487	concentration 4.9 × 10-9 M which is close to normal blood plasma level	469:538	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	4	18	theme	proteins	734:741	arg1	proteins					734:741	the most abundant proteins	716:741	the most abundant proteins in the matrix	716:755	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	4	18	theme	proteins	734:741	arg1	one					709:711	one	709:711	one	709:711	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	4	18	theme	proteins	734:741	arg1	factor					698:703	The Ef-Tu factor	688:703	The Ef-Tu factor	688:703	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	1	19	theme	commensal	165:173	arg1	microbiota					175:184	commensal microbiota	165:184	commensal microbiota	165:184	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	4	20	theme	hormone	805:811	arg1	presence					789:796	the presence	785:796	the presence of the hormone	785:811	The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone.
36204611	2	21	theme	research	304:311	arg1	effects					328:334	the research of epinephrine effects	300:334	the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms	300:396	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	6	22	theme	hormone	1003:1009	arg1	addition					987:994	the addition	983:994	the addition of the hormone	983:1009	The matrix phospholipids were also eradicated by the addition of the hormone.
36204611	2	23	theme	matrix	412:417	arg1	changes					419:425	the matrix changes	408:425	the matrix changes during the biofilm growth	408:451	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	1	24	from	importance	119:128	arg1	microbiota					175:184	commensal microbiota	165:184	commensal microbiota	165:184	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	1	24	from	importance	119:128	arg1	biofilms					200:207	microbial biofilms	190:207	microbial biofilms	190:207	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	0	25	dep	luteus	78:83	arg1	C01					85:87	C01	85:87	Micrococcus luteus C01 isolated from human skin	66:112	Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin.
36204611	1	26	theme	microbial	190:198	arg1	biofilms					200:207	microbial biofilms	190:207	microbial biofilms	190:207	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	3	27	theme	10-9	489:492	arg1	M					494:494	M	494:494	M	494:494	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	1	28	theme	impact	137:142	arg1	importance					119:128	The importance	115:128	The importance of the impact of human hormones on commensal microbiota and microbial biofilms	115:207	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	0	29	theme	matrix	44:49	arg1	composition					51:61	the biofilm matrix composition	32:61	the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin	32:112	Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin.
36204611	0	30	theme	biofilm	36:42	arg1	composition					51:61	the biofilm matrix composition	32:61	the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin	32:112	Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin.
36204611	7	31	from	impact	1043:1048	arg1	composition					1093:1103	their matrix composition	1080:1103	their matrix composition	1080:1103	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	7	31	from	impact	1043:1048	arg1	biofilms					1067:1074	the M. luteus biofilms	1053:1074	the M. luteus biofilms	1053:1074	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	6	32	theme	matrix	938:943	arg1	phospholipids					945:957	The matrix phospholipids	934:957	The matrix phospholipids	934:957	The matrix phospholipids were also eradicated by the addition of the hormone.
36204611	3	33	theme	polysaccharides	564:578	arg1	polysaccharides					564:578	polysaccharides	564:578	polysaccharides	564:578	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	3	33	theme	polysaccharides	564:578	arg1	DNA					598:600	extracellular DNA	584:600	extracellular DNA	584:600	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	3	33	theme	polysaccharides	564:578	arg1	amount					554:559	the amount	550:559	the amount of polysaccharides and extracellular DNA	550:600	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	5	34	from	matrix	874:879	arg1	absent					860:865	absent	860:865	absent	860:865	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	2	35	theme	biofilm	438:444	arg1	growth					446:451	the biofilm growth	434:451	the biofilm growth	434:451	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	5	36	theme	glucose-mannose	825:839	arg1	polysaccharide					841:854	the glucose-mannose polysaccharide	821:854	the glucose-mannose polysaccharide	821:854	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	7	37	theme	future	1152:1157	arg1	research					1159:1166	the future research	1148:1166	the future research	1148:1166	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	3	38	theme	concentration	469:481	arg1	×					487:487	concentration 4.9 ×	469:487	concentration 4.9 × 10-9 M which is close to normal blood plasma level	469:538	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	5	39	theme	epinephrine	896:906	arg1	presence					884:891	presence	884:891	presence of epinephrine	884:906	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	7	40	contain	has	1031:1033	arg1	epinephrine					1019:1029	epinephrine	1019:1029	epinephrine	1019:1029	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	7	40	contain	has	1031:1033	arg2	impact					1043:1048	a great impact	1035:1048	a great impact on the M. luteus biofilms and their matrix composition	1035:1103	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	7	41	theme	matrix	1086:1091	arg1	composition					1093:1103	their matrix composition	1080:1103	their matrix composition	1080:1103	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	3	42	theme	DNA	598:600	arg1	polysaccharides					564:578	polysaccharides	564:578	polysaccharides	564:578	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	3	42	theme	DNA	598:600	arg1	DNA					598:600	extracellular DNA	584:600	extracellular DNA	584:600	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	3	42	theme	DNA	598:600	arg1	amount					554:559	the amount	550:559	the amount of polysaccharides and extracellular DNA	550:600	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	2	43	theme	present	251:257	arg1	investigation					259:271	the present investigation	247:271	the present investigation	247:271	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	1	44	from	biofilms	200:207	arg1	importance					119:128	The importance	115:128	The importance of the impact of human hormones on commensal microbiota and microbial biofilms	115:207	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	2	45	theme	epinephrine	316:326	arg1	effects					328:334	the research of epinephrine effects	300:334	the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms	300:396	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	3	46	theme	blood	521:525	arg1	level					534:538	normal blood plasma level	514:538	normal blood plasma level	514:538	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	0	47	attach	isolated	89:96	arg1	skin					109:112	human skin	103:112	human skin	103:112	Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin.
36204611	0	47	attach	isolated	89:96	arg2	luteus					78:83	Micrococcus luteus	66:83	Micrococcus luteus C01 isolated from human skin	66:112	Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin.
36204611	1	48	from	microbiota	175:184	arg1	importance					119:128	The importance	115:128	The importance of the impact of human hormones on commensal microbiota and microbial biofilms	115:207	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	2	49	theme	of	313:314	arg1	effects					328:334	the research of epinephrine effects	300:334	the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms	300:396	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	5	50	from	presence	884:891	arg1	absent					860:865	absent	860:865	absent	860:865	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	5	51	theme	polysaccharide	841:854	arg1	One					814:816	One	814:816	One	814:816	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	5	51	theme	polysaccharide	841:854	arg1	polysaccharide					841:854	the glucose-mannose polysaccharide	821:854	the glucose-mannose polysaccharide	821:854	One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation.
36204611	2	52	theme	Micrococcus	358:368	arg1	luteus					370:375	the skin commensal Micrococcus luteus C01	339:379	the skin commensal Micrococcus luteus C01	339:379	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	7	53	theme	luteus	1060:1065	arg1	biofilms					1067:1074	the M. luteus biofilms	1053:1074	the M. luteus biofilms	1053:1074	Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.
36204611	3	54	theme	polysaccharide	660:673	arg1	composition					675:685	polysaccharide composition	660:685	polysaccharide composition	660:685	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	3	54	theme	polysaccharide	660:673	arg1	protein					641:647	its protein	637:647	its protein	637:647	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	3	55	theme	extracellular	584:596	arg1	DNA					598:600	extracellular DNA	584:600	extracellular DNA	584:600	Epinephrine in concentration 4.9 × 10-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition.
36204611	2	56	theme	commensal	348:356	arg1	luteus					370:375	the skin commensal Micrococcus luteus C01	339:379	the skin commensal Micrococcus luteus C01	339:379	In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth.
36204611	1	57	theme	studies	235:241	arg1	studies					235:241	studies	235:241	studies	235:241	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36204611	1	57	theme	studies	235:241	arg1	lots					227:230	lots	227:230	lots of studies	227:241	The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies.
36891384	9	0	dep	tpa	2284:2286	arg1	gdh					2294:2296	gdh	2294:2296	gdh	2294:2296	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	0	dep	tpa	2284:2286	arg1	betC					2303:2306	betC	2303:2306	betC	2303:2306	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	0	dep	tpa	2284:2286	arg1	mdh					2289:2291	mdh	2289:2291	mdh	2289:2291	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	5	1	dep	phyla	1068:1072	arg1	phyla					1068:1072	four phyla	1063:1072	four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria)	1063:1139	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	5	1	dep	phyla	1068:1072	arg1	Chloroflexi					1109:1119	Chloroflexi	1109:1119	Chloroflexi	1109:1119	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	5	1	dep	phyla	1068:1072	arg1	Cyanobacteria					1126:1138	Cyanobacteria	1126:1138	Cyanobacteria	1126:1138	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	5	1	dep	phyla	1068:1072	arg1	Gemmatimonadetes					1091:1106	Gemmatimonadetes	1091:1106	Gemmatimonadetes	1091:1106	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	6	2	theme	enriched	1291:1298	arg1	functions					1300:1308	The differentially enriched functions	1272:1308	The differentially enriched functions (KEGG level 2) in 101-14	1272:1333	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	2	theme	enriched	1291:1298	arg1	level					1316:1320	KEGG level 2	1311:1322	KEGG level 2	1311:1322	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	10	3	dep	composition	2492:2502	arg1	the					2488:2490	the	2488:2490	the	2488:2490	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	9	4	dep	transformation	2268:2281	arg1	tpa					2284:2286	tpa	2284:2286	tpa	2284:2286	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	4	5	theme	salt	697:700	arg1	stress					702:707	salt stress	697:707	salt stress	697:707	Results and Discussion Compared to the control (treated with ddH2O), salt stress induced greater changes in the rhizosphere microbiota of 101-14 than in that of 5BB.
36891384	8	6	theme	salt	1934:1937	arg1	stress					1939:1944	salt stress	1934:1944	salt stress	1934:1944	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	3	7	theme	salt	615:618	arg1	stress					620:625	salt stress	615:625	salt stress	615:625	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	10	8	theme	grapevines	2602:2611	arg1	tolerance					2584:2592	the enhanced tolerance	2571:2592	the enhanced tolerance of some grapevines to salt stress	2571:2626	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	9	9	theme	sulfur	2185:2190	arg1	reduction					2192:2200	sulfur reduction	2185:2200	sulfur reduction (fsr)	2185:2206	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	9	theme	sulfur	2185:2190	arg1	reduction					2146:2154	assimilatory sulfate reduction	2125:2154	assimilatory sulfate reduction (cysNC, cysQ, sat, and sir)	2125:2182	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	9	theme	sulfur	2185:2190	arg1	fsr					2203:2205	fsr	2203:2205	fsr	2203:2205	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	8	10	from	mitigation	1990:1999	arg1	grapevines					2019:2028	grapevines	2019:2028	grapevines	2019:2028	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	8	11	theme	vital	1971:1975	arg1	roles					1977:1981	vital roles	1971:1981	vital roles	1971:1981	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	5	12	theme	phyla	1068:1072	arg1	abundances					1049:1058	only the relative abundances	1031:1058	only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria)	1031:1139	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	2	13	theme	plants	249:254	arg1	microbiota					235:244	The rhizosphere microbiota	219:244	The rhizosphere microbiota of plants	219:254	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	1	14	theme	Introduction	144:155	arg1	stress					196:201	a serious abiotic stress	178:201	a serious abiotic stress for grapevines	178:216	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	1	14	theme	Introduction	144:155	arg1	salinization					162:173	Introduction Soil salinization	144:173	Introduction Soil salinization	144:173	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	6	15	theme	translation	1581:1591	arg1	function					1593:1600	only the translation function	1572:1600	only the translation function	1572:1600	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	7	16	theme	related	1750:1756	arg1	pathways					1741:1748	pathways	1741:1748	pathways related to metabolism	1741:1770	Under salt stress, the rhizosphere microbiota functions of 101-14 and 5BB differed greatly, especially pathways related to metabolism.
36891384	10	17	theme	salt	2616:2619	arg1	stress					2621:2626	salt stress	2616:2626	salt stress	2616:2626	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	5	18	dep	increased	984:992	arg1	whereas					1023:1029	whereas	1023:1029	whereas	1023:1029	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	9	19	theme	SOX	2209:2211	arg1	soxB					2222:2225	soxB	2222:2225	soxB	2222:2225	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	19	theme	SOX	2209:2211	arg1	reduction					2146:2154	assimilatory sulfate reduction	2125:2154	assimilatory sulfate reduction (cysNC, cysQ, sat, and sir)	2125:2182	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	19	theme	SOX	2209:2211	arg1	systems					2213:2219	SOX systems	2209:2219	SOX systems (soxB)	2209:2226	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	6	20	theme	degradation	1420:1430	arg1	functions					1432:1440	degradation functions	1420:1440	degradation functions	1420:1440	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	10	21	theme	study	2454:2458	arg1	findings					2460:2467	the study findings	2450:2467	the study findings	2450:2467	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	6	22	theme	cell	1383:1386	arg1	motility					1388:1395	cell motility	1383:1395	cell motility	1383:1395	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	8	23	theme	glutathione	1840:1850	arg1	metabolism					1852:1861	glutathione metabolism	1840:1861	glutathione metabolism	1840:1861	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	1	24	theme	abiotic	188:194	arg1	stress					196:201	a serious abiotic stress	178:201	a serious abiotic stress for grapevines	178:216	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	1	24	theme	abiotic	188:194	arg1	salinization					162:173	Introduction Soil salinization	144:173	Introduction Soil salinization	144:173	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	3	25	dep	Methods	429:435	arg1	employed					448:455	employed	448:455	employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress	448:625	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	6	26	theme	related	1372:1378	arg1	pathways					1363:1370	pathways	1363:1370	pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins	1363:1561	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	3	27	dep	101-14	551:556	arg1	tolerant					564:571	tolerant	564:571	tolerant	564:571	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	7	28	theme	5BB	1708:1710	arg1	functions					1684:1692	the rhizosphere microbiota functions	1657:1692	the rhizosphere microbiota functions of 101-14 and 5BB	1657:1710	Under salt stress, the rhizosphere microbiota functions of 101-14 and 5BB differed greatly, especially pathways related to metabolism.
36891384	9	29	theme	sulfur	2229:2234	arg1	reduction					2146:2154	assimilatory sulfate reduction	2125:2154	assimilatory sulfate reduction (cysNC, cysQ, sat, and sir)	2125:2182	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	29	theme	sulfur	2229:2234	arg1	sqr					2247:2249	sqr	2247:2249	sqr	2247:2249	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	29	theme	sulfur	2229:2234	arg1	oxidation					2236:2244	sulfur oxidation	2229:2244	sulfur oxidation (sqr)	2229:2250	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	0	30	theme	salt	131:134	arg1	stress					136:141	salt stress	131:141	salt stress	131:141	Comparative metagenomic analysis reveals rhizosphere microbial community composition and functions help protect grapevines against salt stress.
36891384	9	31	theme	salt	2421:2424	arg1	effects					2410:2416	the harmful effects	2398:2416	the harmful effects of salt on grapevine	2398:2437	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	7	32	theme	101-14	1697:1702	arg1	functions					1684:1692	the rhizosphere microbiota functions	1657:1692	the rhizosphere microbiota functions of 101-14 and 5BB	1657:1710	Under salt stress, the rhizosphere microbiota functions of 101-14 and 5BB differed greatly, especially pathways related to metabolism.
36891384	9	33	theme	various	2061:2067	arg1	genes					2090:2094	various sulfur cycle-related genes	2061:2094	various sulfur cycle-related genes	2061:2094	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	33	theme	various	2061:2067	arg1	genes					2107:2111	genes	2107:2111	genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC),	2107:2308	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	4	34	theme	101-14	766:771	arg1	microbiota					752:761	the rhizosphere microbiota	736:761	the rhizosphere microbiota of 101-14	736:771	Results and Discussion Compared to the control (treated with ddH2O), salt stress induced greater changes in the rhizosphere microbiota of 101-14 than in that of 5BB.
36891384	0	35	theme	Comparative	0:10	arg1	analysis					24:31	Comparative metagenomic analysis	0:31	Comparative metagenomic analysis	0:31	Comparative metagenomic analysis reveals rhizosphere microbial community composition and functions help protect grapevines against salt stress.
36891384	8	36	from	enriched	1909:1916	arg1	101-14					1921:1926	101-14	1921:1926	101-14	1921:1926	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	9	37	theme	cycle-related	2076:2088	arg1	genes					2090:2094	various sulfur cycle-related genes	2061:2094	various sulfur cycle-related genes	2061:2094	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	37	theme	cycle-related	2076:2088	arg1	genes					2107:2111	genes	2107:2111	genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC),	2107:2308	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	5	38	theme	bacteria	849:856	arg1	abundances					807:816	The relative abundances	794:816	The relative abundances	794:816	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	3	39	dep	5BB	578:580	arg1	sensitive					588:596	sensitive	588:596	sensitive	588:596	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	7	40	theme	rhizosphere	1661:1671	arg1	functions					1684:1692	the rhizosphere microbiota functions	1657:1692	the rhizosphere microbiota functions of 101-14 and 5BB	1657:1710	Under salt stress, the rhizosphere microbiota functions of 101-14 and 5BB differed greatly, especially pathways related to metabolism.
36891384	3	41	theme	metagenomic	457:467	arg1	sequencing					469:478	metagenomic sequencing	457:478	metagenomic sequencing	457:478	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	3	42	theme	microbial	507:515	arg1	community					517:525	the rhizosphere microbial community	491:525	the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress	491:625	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	9	43	theme	organic	2253:2259	arg1	transformation					2268:2281	organic sulfur transformation	2253:2281	organic sulfur transformation (tpa, mdh, gdh, and betC)	2253:2307	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	43	theme	organic	2253:2259	arg1	reduction					2146:2154	assimilatory sulfate reduction	2125:2154	assimilatory sulfate reduction (cysNC, cysQ, sat, and sir)	2125:2182	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	0	44	theme	rhizosphere	41:51	arg1	composition					73:83	rhizosphere microbial community composition	41:83	rhizosphere microbial community composition	41:83	Comparative metagenomic analysis reveals rhizosphere microbial community composition and functions help protect grapevines against salt stress.
36891384	5	45	theme	salt	1169:1172	arg1	stress					1174:1179	salt stress	1169:1179	salt stress	1169:1179	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	5	46	theme	relative	798:805	arg1	abundances					807:816	The relative abundances	794:816	The relative abundances	794:816	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	10	47	theme	microbial	2537:2545	arg1	community					2547:2555	the rhizosphere microbial community	2521:2555	the rhizosphere microbial community	2521:2555	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	2	48	theme	salt-tolerant	369:381	arg1	varieties					402:410	salt-tolerant and salt-sensitive varieties	369:410	salt-tolerant and salt-sensitive varieties	369:410	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	0	49	theme	community	63:71	arg1	composition					73:83	rhizosphere microbial community composition	41:83	rhizosphere microbial community composition	41:83	Comparative metagenomic analysis reveals rhizosphere microbial community composition and functions help protect grapevines against salt stress.
36891384	8	50	from	101-14	1921:1926	arg1	enriched					1909:1916	enriched	1909:1916	enriched	1909:1916	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	5	51	dep	Planctomycetes	869:882	arg1	Bacteroidetes					885:897	Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes	869:976	Bacteroidetes	885:897	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	3	52	theme	rootstocks	540:549	arg1	101-14					551:556	grapevine rootstocks 101-14	530:556	grapevine rootstocks 101-14 (salt tolerant)	530:572	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	6	53	from	functions	1300:1308	arg1	101-14					1328:1333	101-14	1328:1333	101-14	1328:1333	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	9	54	theme	sulfate	2138:2144	arg1	reduction					2192:2200	sulfur reduction	2185:2200	sulfur reduction (fsr)	2185:2206	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	54	theme	sulfate	2138:2144	arg1	reduction					2146:2154	assimilatory sulfate reduction	2125:2154	assimilatory sulfate reduction (cysNC, cysQ, sat, and sir)	2125:2182	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	54	theme	sulfate	2138:2144	arg1	systems					2213:2219	SOX systems	2209:2219	SOX systems (soxB)	2209:2226	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	54	theme	sulfate	2138:2144	arg1	transformation					2268:2281	organic sulfur transformation	2253:2281	organic sulfur transformation (tpa, mdh, gdh, and betC)	2253:2307	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	54	theme	sulfate	2138:2144	arg1	oxidation					2236:2244	sulfur oxidation	2229:2244	sulfur oxidation (sqr)	2229:2250	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	6	55	theme	KEGG	1311:1314	arg1	functions					1300:1308	The differentially enriched functions	1272:1308	The differentially enriched functions (KEGG level 2) in 101-14	1272:1333	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	55	theme	KEGG	1311:1314	arg1	level					1316:1320	KEGG level 2	1311:1322	KEGG level 2	1311:1322	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	2	56	theme	salt-sensitive	387:400	arg1	varieties					402:410	salt-tolerant and salt-sensitive varieties	369:410	salt-tolerant and salt-sensitive varieties	369:410	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	2	57	theme	salt	304:307	arg1	stress					309:314	salt stress	304:314	salt stress	304:314	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	6	58	theme	vitamins	1554:1561	arg1	biosynthesis					1450:1461	glycan biosynthesis	1443:1461	glycan biosynthesis	1443:1461	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	metabolism					1467:1476	metabolism	1467:1476	metabolism	1467:1476	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	motility					1388:1395	cell motility	1383:1395	cell motility	1383:1395	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	folding					1398:1404	folding	1398:1404	folding	1398:1404	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	metabolism					1526:1535	metabolism	1526:1535	metabolism of cofactors and vitamins	1526:1561	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	functions					1432:1440	degradation functions	1420:1440	degradation functions	1420:1440	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	xenobiotics					1479:1489	xenobiotics biodegradation and metabolism	1479:1519	xenobiotics biodegradation and metabolism	1479:1519	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	biodegradation					1491:1504	biodegradation	1491:1504	biodegradation	1491:1504	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	sorting					1407:1413	sorting	1407:1413	sorting	1407:1413	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	58	theme	vitamins	1554:1561	arg1	metabolism					1510:1519	metabolism	1510:1519	metabolism	1510:1519	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	8	59	theme	salt	2004:2007	arg1	stress					2009:2014	salt stress	2004:2014	salt stress	2004:2014	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	6	60	theme	cofactors	1540:1548	arg1	biosynthesis					1450:1461	glycan biosynthesis	1443:1461	glycan biosynthesis	1443:1461	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	metabolism					1467:1476	metabolism	1467:1476	metabolism	1467:1476	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	motility					1388:1395	cell motility	1383:1395	cell motility	1383:1395	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	folding					1398:1404	folding	1398:1404	folding	1398:1404	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	metabolism					1526:1535	metabolism	1526:1535	metabolism of cofactors and vitamins	1526:1561	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	functions					1432:1440	degradation functions	1420:1440	degradation functions	1420:1440	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	xenobiotics					1479:1489	xenobiotics biodegradation and metabolism	1479:1519	xenobiotics biodegradation and metabolism	1479:1519	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	biodegradation					1491:1504	biodegradation	1491:1504	biodegradation	1491:1504	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	sorting					1407:1413	sorting	1407:1413	sorting	1407:1413	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	60	theme	cofactors	1540:1548	arg1	metabolism					1510:1519	metabolism	1510:1519	metabolism	1510:1519	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	9	61	dep	cysNC	2157:2161	arg1	sat					2170:2172	sat	2170:2172	sat	2170:2172	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	61	dep	cysNC	2157:2161	arg1	sir					2179:2181	sir	2179:2181	sir	2179:2181	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	61	dep	cysNC	2157:2161	arg1	cysQ					2164:2167	cysQ	2164:2167	cysQ	2164:2167	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	7	62	theme	salt	1644:1647	arg1	stress					1649:1654	salt stress	1644:1654	salt stress	1644:1654	Under salt stress, the rhizosphere microbiota functions of 101-14 and 5BB differed greatly, especially pathways related to metabolism.
36891384	8	63	theme	Further	1773:1779	arg1	analysis					1781:1788	Further analysis	1773:1788	Further analysis	1773:1788	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	1	64	theme	Soil	157:160	arg1	stress					196:201	a serious abiotic stress	178:201	a serious abiotic stress for grapevines	178:216	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	1	64	theme	Soil	157:160	arg1	salinization					162:173	Introduction Soil salinization	144:173	Introduction Soil salinization	144:173	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	4	65	theme	rhizosphere	740:750	arg1	microbiota					752:761	the rhizosphere microbiota	736:761	the rhizosphere microbiota of 101-14	736:771	Results and Discussion Compared to the control (treated with ddH2O), salt stress induced greater changes in the rhizosphere microbiota of 101-14 than in that of 5BB.
36891384	2	66	theme	negative	277:284	arg1	effects					286:292	the negative effects	273:292	the negative effects caused by salt stress	273:314	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	5	67	dep	phyla	1202:1206	arg1	Verrucomicrobia					1224:1238	Verrucomicrobia	1224:1238	Verrucomicrobia	1224:1238	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	5	67	dep	phyla	1202:1206	arg1	Firmicutes					1245:1254	Firmicutes	1245:1254	Firmicutes	1245:1254	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	5	67	dep	phyla	1202:1206	arg1	phyla					1202:1206	three phyla	1196:1206	three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes)	1196:1255	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	8	68	theme	stress	2009:2014	arg1	mitigation					1990:1999	the mitigation	1986:1999	the mitigation of salt stress on grapevines	1986:2028	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	9	69	dep	reduction	2146:2154	arg1	cysNC					2157:2161	cysNC	2157:2161	cysNC	2157:2161	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	1	70	theme	serious	180:186	arg1	stress					196:201	a serious abiotic stress	178:201	a serious abiotic stress for grapevines	178:216	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	1	70	theme	serious	180:186	arg1	salinization					162:173	Introduction Soil salinization	144:173	Introduction Soil salinization	144:173	Introduction Soil salinization is a serious abiotic stress for grapevines.
36891384	2	71	theme	rhizosphere	223:233	arg1	microbiota					235:244	The rhizosphere microbiota	219:244	The rhizosphere microbiota of plants	219:254	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	4	72	theme	greater	717:723	arg1	changes					725:731	greater changes	717:731	greater changes in the rhizosphere microbiota of 101-14	717:771	Results and Discussion Compared to the control (treated with ddH2O), salt stress induced greater changes in the rhizosphere microbiota of 101-14 than in that of 5BB.
36891384	9	73	theme	harmful	2402:2408	arg1	effects					2410:2416	the harmful effects	2398:2416	the harmful effects of salt on grapevine	2398:2437	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	2	74	theme	rhizosphere	345:355	arg1	microbes					357:364	rhizosphere microbes	345:364	rhizosphere microbes of salt-tolerant and salt-sensitive varieties	345:410	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	5	75	theme	salt	1010:1013	arg1	stress					1015:1020	salt stress	1010:1020	salt stress	1010:1020	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	9	76	with	treatment	2350:2358	arg1	NaCl					2365:2368	NaCl	2365:2368	NaCl	2365:2368	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	3	77	theme	rhizosphere	495:505	arg1	community					517:525	the rhizosphere microbial community	491:525	the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress	491:625	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	0	78	theme	metagenomic	12:22	arg1	analysis					24:31	Comparative metagenomic analysis	0:31	Comparative metagenomic analysis	0:31	Comparative metagenomic analysis reveals rhizosphere microbial community composition and functions help protect grapevines against salt stress.
36891384	9	79	theme	sulfur	2069:2074	arg1	genes					2090:2094	various sulfur cycle-related genes	2061:2094	various sulfur cycle-related genes	2061:2094	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	79	theme	sulfur	2069:2074	arg1	genes					2107:2111	genes	2107:2111	genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC),	2107:2308	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	5	80	theme	growth-promoting	832:847	arg1	bacteria					849:856	more plant growth-promoting bacteria	821:856	more plant growth-promoting bacteria	821:856	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	8	81	theme	bacterial	1874:1882	arg1	chemotaxis					1884:1893	bacterial chemotaxis	1874:1893	bacterial chemotaxis	1874:1893	Further analysis revealed that pathways associated with sulfur and glutathione metabolism as well as bacterial chemotaxis were uniquely enriched in 101-14 under salt stress and therefore might play vital roles in the mitigation of salt stress on grapevines.
36891384	6	82	theme	glycan	1443:1448	arg1	biosynthesis					1450:1461	glycan biosynthesis	1443:1461	glycan biosynthesis	1443:1461	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	9	83	theme	genes	2090:2094	arg1	abundance					2048:2056	the abundance	2044:2056	the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC),	2044:2308	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	3	84	theme	grapevine	530:538	arg1	101-14					551:556	grapevine rootstocks 101-14	530:556	grapevine rootstocks 101-14 (salt tolerant)	530:572	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	0	85	theme	microbial	53:61	arg1	composition					73:83	rhizosphere microbial community composition	41:83	rhizosphere microbial community composition	41:83	Comparative metagenomic analysis reveals rhizosphere microbial community composition and functions help protect grapevines against salt stress.
36891384	5	86	theme	relative	1040:1047	arg1	abundances					1049:1058	only the relative abundances	1031:1058	only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria)	1031:1139	The relative abundances of more plant growth-promoting bacteria, including Planctomycetes, Bacteroidetes, Verrucomicrobia, Cyanobacteria, Gemmatimonadetes, Chloroflexi, and Firmicutes, were increased in 101-14 under salt stress, whereas only the relative abundances of four phyla (Actinobacteria, Gemmatimonadetes, Chloroflexi, and Cyanobacteria) were increased in 5BB under salt stress while those of three phyla (Acidobacteria, Verrucomicrobia, and Firmicutes) were depleted.
36891384	7	87	theme	microbiota	1673:1682	arg1	functions					1684:1692	the rhizosphere microbiota functions	1657:1692	the rhizosphere microbiota functions of 101-14 and 5BB	1657:1710	Under salt stress, the rhizosphere microbiota functions of 101-14 and 5BB differed greatly, especially pathways related to metabolism.
36891384	10	88	theme	community	2547:2555	arg1	functions					2508:2516	functions	2508:2516	functions	2508:2516	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	10	88	theme	community	2547:2555	arg1	composition					2492:2502	composition	2492:2502	composition	2492:2502	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	9	89	theme	sulfur	2261:2266	arg1	transformation					2268:2281	organic sulfur transformation	2253:2281	organic sulfur transformation (tpa, mdh, gdh, and betC)	2253:2307	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	89	theme	sulfur	2261:2266	arg1	reduction					2146:2154	assimilatory sulfate reduction	2125:2154	assimilatory sulfate reduction (cysNC, cysQ, sat, and sir)	2125:2182	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	90	from	effects	2410:2416	arg1	grapevine					2429:2437	grapevine	2429:2437	grapevine	2429:2437	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	2	91	theme	varieties	402:410	arg1	microbes					357:364	rhizosphere microbes	345:364	rhizosphere microbes of salt-tolerant and salt-sensitive varieties	345:410	The rhizosphere microbiota of plants can help counter the negative effects caused by salt stress, but the distinction between rhizosphere microbes of salt-tolerant and salt-sensitive varieties remains unclear.
36891384	10	92	theme	rhizosphere	2525:2535	arg1	community					2547:2555	the rhizosphere microbial community	2521:2555	the rhizosphere microbial community	2521:2555	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	9	93	theme	assimilatory	2125:2136	arg1	reduction					2192:2200	sulfur reduction	2185:2200	sulfur reduction (fsr)	2185:2206	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	93	theme	assimilatory	2125:2136	arg1	reduction					2146:2154	assimilatory sulfate reduction	2125:2154	assimilatory sulfate reduction (cysNC, cysQ, sat, and sir)	2125:2182	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	93	theme	assimilatory	2125:2136	arg1	systems					2213:2219	SOX systems	2209:2219	SOX systems (soxB)	2209:2226	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	93	theme	assimilatory	2125:2136	arg1	transformation					2268:2281	organic sulfur transformation	2253:2281	organic sulfur transformation (tpa, mdh, gdh, and betC)	2253:2307	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	9	93	theme	assimilatory	2125:2136	arg1	oxidation					2236:2244	sulfur oxidation	2229:2244	sulfur oxidation (sqr)	2229:2250	In addition, the abundance of various sulfur cycle-related genes, including genes involved in assimilatory sulfate reduction (cysNC, cysQ, sat, and sir), sulfur reduction (fsr), SOX systems (soxB), sulfur oxidation (sqr), organic sulfur transformation (tpa, mdh, gdh, and betC), increased significantly in 101-14 after treatment with NaCl; these genes might mitigate the harmful effects of salt on grapevine.
36891384	10	94	theme	enhanced	2575:2582	arg1	tolerance					2584:2592	the enhanced tolerance	2571:2592	the enhanced tolerance of some grapevines to salt stress	2571:2626	In short, the study findings indicate that both the composition and functions of the rhizosphere microbial community contribute to the enhanced tolerance of some grapevines to salt stress.
36891384	3	95	theme	101-14	551:556	arg1	community					517:525	the rhizosphere microbial community	491:525	the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress	491:625	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	6	96	dep	xenobiotics	1479:1489	arg1	biodegradation					1491:1504	biodegradation	1491:1504	biodegradation	1491:1504	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	96	dep	xenobiotics	1479:1489	arg1	xenobiotics					1479:1489	xenobiotics biodegradation and metabolism	1479:1519	xenobiotics biodegradation and metabolism	1479:1519	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	6	96	dep	xenobiotics	1479:1489	arg1	metabolism					1510:1519	metabolism	1510:1519	metabolism	1510:1519	The differentially enriched functions (KEGG level 2) in 101-14 were mainly associated with pathways related to cell motility; folding, sorting, and degradation functions; glycan biosynthesis and metabolism; xenobiotics biodegradation and metabolism; and metabolism of cofactors and vitamins, whereas only the translation function was differentially enriched in 5BB.
36891384	3	97	theme	5BB	578:580	arg1	community					517:525	the rhizosphere microbial community	491:525	the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress	491:625	Methods This study employed metagenomic sequencing to explore the rhizosphere microbial community of grapevine rootstocks 101-14 (salt tolerant) and 5BB (salt sensitive) with or without salt stress.
36891384	4	98	from	changes	725:731	arg1	microbiota					752:761	the rhizosphere microbiota	736:761	the rhizosphere microbiota of 101-14	736:771	Results and Discussion Compared to the control (treated with ddH2O), salt stress induced greater changes in the rhizosphere microbiota of 101-14 than in that of 5BB.
35051823	8	0	theme	yield	1835:1839	arg1	minimization					1841:1852	the sludge yield minimization	1824:1852	the sludge yield minimization from MANODOX like systems	1824:1878	The observations confirmed that the sludge yield minimization from MANODOX like systems could be effectively controlled by maintaining aforementioned operational tactics.
35051823	7	1	theme	feed	1777:1780	arg1	influent					1782:1789	feed influent	1777:1789	feed influent	1777:1789	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	6	2	theme	SRT	1255:1257	arg1	range					1246:1250	favoring range	1237:1250	favoring range of SRT (24 days) for MANODOX implementation	1237:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	3	3	theme	influent	722:729	arg1	treatment					696:704	the treatment	692:704	the treatment of real tannery influent	692:729	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	1	4	theme	biological	200:209	arg1	treatment					211:219	advanced biological treatment for industrial wastewater could be considered a poorly explored area	191:288	advanced biological treatment for industrial wastewater could be considered a poorly explored area	191:288	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	8	5	theme	aforementioned	1927:1940	arg1	tactics					1954:1960	aforementioned operational tactics	1927:1960	aforementioned operational tactics	1927:1960	The observations confirmed that the sludge yield minimization from MANODOX like systems could be effectively controlled by maintaining aforementioned operational tactics.
35051823	8	6	theme	sludge	1828:1833	arg1	minimization					1841:1852	the sludge yield minimization	1824:1852	the sludge yield minimization from MANODOX like systems	1824:1878	The observations confirmed that the sludge yield minimization from MANODOX like systems could be effectively controlled by maintaining aforementioned operational tactics.
35051823	6	7	theme	outstanding	1309:1319	arg1	minimization					1342:1353	an outstanding in-situ sludge yield minimization	1306:1353	an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment	1306:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	8	theme	higher	1482:1487	arg1	salinity					1489:1496	higher salinity	1482:1496	higher salinity of the mixed liquid	1482:1516	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	8	theme	higher	1482:1487	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	9	theme	strategies	1216:1225	arg1	maintenance					1181:1191	The strict maintenance	1170:1191	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation	1170:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	5	10	theme	facultative	1049:1059	arg1	microbiota					1061:1070	facultative microbiota	1049:1070	facultative microbiota	1049:1070	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	6	11	theme	run	1577:1579	arg1	treatment					1599:1607	a parallel run identical aerobic treatment	1566:1607	a parallel run identical aerobic treatment	1566:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	1	12	theme	serious	308:314	arg1	attention					316:324	serious attention	308:324	serious attention of the scientific community	308:352	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	6	13	theme	adapted	1196:1202	arg1	range					1246:1250	favoring range	1237:1250	favoring range of SRT (24 days) for MANODOX implementation	1237:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	13	theme	adapted	1196:1202	arg1	strategies					1216:1225	adapted operational strategies	1196:1225	adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation	1196:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	14	theme	MANODOX	1273:1279	arg1	implementation					1281:1294	MANODOX implementation	1273:1294	MANODOX implementation	1273:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	7	15	theme	sludge	1729:1734	arg1	ratio					1768:1772	carbon to nitrogen ratio	1749:1772	carbon to nitrogen ratio of feed influent	1749:1789	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	7	15	theme	sludge	1729:1734	arg1	ratio					1742:1746	sludge PN/PS ratio	1729:1746	sludge PN/PS ratio	1729:1746	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	5	16	theme	volume	1031:1036	arg1	index					1038:1042	sludge volume index	1024:1042	sludge volume index	1024:1042	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	3	17	theme	mechanism	632:640	arg1	elucidation					601:611	the elucidation	597:611	the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent	597:729	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	1	18	from	treatment	211:219	arg1	minimization					173:184	The sludge yield minimization	156:184	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area	156:288	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	2	19	theme	real	511:514	arg1	wastewater					524:533	real tannery wastewater	511:533	real tannery wastewater	511:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	0	20	theme	minimization	65:76	arg1	performance					78:88	in-situ sludge minimization performance	50:88	in-situ sludge minimization performance of upgraded	50:100	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	5	21	theme	hydrodynamic	1099:1110	arg1	SRT					1135:1137	SRT	1135:1137	SRT	1135:1137	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	21	theme	hydrodynamic	1099:1110	arg1	time					1129:1132	hydrodynamic sludge retention time	1099:1132	hydrodynamic sludge retention time (SRT) of 08-40 days	1099:1152	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	3	22	theme	EPS	616:618	arg1	mechanism					632:640	EPS degradation mechanism	616:640	EPS degradation mechanism	616:640	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	6	23	theme	cell-to-cell	1528:1539	arg1	attachment					1541:1550	weakened cell-to-cell attachment	1519:1550	weakened cell-to-cell attachment	1519:1550	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	23	theme	cell-to-cell	1528:1539	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	1	24	theme	yield	167:171	arg1	minimization					173:184	The sludge yield minimization	156:184	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area	156:288	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	2	25	theme	desired	563:569	arg1	manner					571:576	a desired manner	561:576	a desired manner	561:576	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	5	26	theme	retention	1119:1127	arg1	SRT					1135:1137	SRT	1135:1137	SRT	1135:1137	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	26	theme	retention	1119:1127	arg1	time					1129:1132	hydrodynamic sludge retention time	1099:1132	hydrodynamic sludge retention time (SRT) of 08-40 days	1099:1152	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	0	27	from	Effect	0:5	arg1	performance					78:88	in-situ sludge minimization performance	50:88	in-situ sludge minimization performance of upgraded	50:100	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	4	28	theme	extraction	766:775	arg1	method					777:782	a modified heat extraction method	750:782	a modified heat extraction method	750:782	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	6	29	theme	sludge	1329:1334	arg1	minimization					1342:1353	an outstanding in-situ sludge yield minimization	1306:1353	an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment	1306:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	7	30	theme	corresponding	1659:1671	arg1	decline					1673:1679	corresponding decline	1659:1679	corresponding decline in floc aggregation	1659:1699	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	2	31	theme	sludge	470:475	arg1	treatment					477:485	an upgraded activated sludge treatment	448:485	an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater	448:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	2	32	theme	polymeric	402:410	arg1	substances					412:421	extracellular polymeric substances	388:421	the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater	384:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	2	32	theme	polymeric	402:410	arg1	EPS					424:426	EPS	424:426	EPS	424:426	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	6	33	dep	0.39	1369:1372	arg1	to					1366:1367	to	1366:1367	to	1366:1367	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	34	theme	aerobic	1591:1597	arg1	treatment					1599:1607	a parallel run identical aerobic treatment	1566:1607	a parallel run identical aerobic treatment	1566:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	2	35	theme	upgraded	451:458	arg1	treatment					477:485	an upgraded activated sludge treatment	448:485	an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater	448:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	4	36	theme	modified	752:759	arg1	method					777:782	a modified heat extraction method	750:782	a modified heat extraction method	750:782	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	0	37	theme	extracellular	10:22	arg1	compositions					34:45	extracellular polymeric compositions	10:45	extracellular polymeric compositions	10:45	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	2	38	theme	MANODOX	491:497	arg1	system					499:504	MANODOX system	491:504	MANODOX system	491:504	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	4	39	theme	EPS	819:821	arg1	fractions					823:831	EPS fractions	819:831	EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge	819:906	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	0	40	theme	compositions	34:45	arg1	Effect					0:5	Effect	0:5	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded	0:100	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	3	41	theme	floc	646:649	arg1	morphology					651:660	floc morphology	646:660	floc morphology	646:660	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	0	42	theme	in-situ	50:56	arg1	performance					78:88	in-situ sludge minimization performance	50:88	in-situ sludge minimization performance of upgraded	50:100	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	1	43	theme	community	344:352	arg1	attention					316:324	serious attention	308:324	serious attention of the scientific community	308:352	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	6	44	theme	PN/PS	1469:1473	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	44	theme	PN/PS	1469:1473	arg1	ratio					1475:1479	comparably lower PN/PS ratio	1452:1479	comparably lower PN/PS ratio	1452:1479	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	5	45	theme	PN/PS	988:992	arg1	ratio					994:998	PN/PS ratio	988:998	PN/PS ratio	988:998	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	7	46	theme	influent	1782:1789	arg1	ratio					1768:1772	carbon to nitrogen ratio	1749:1772	carbon to nitrogen ratio of feed influent	1749:1789	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	7	46	theme	influent	1782:1789	arg1	ratio					1742:1746	sludge PN/PS ratio	1729:1746	sludge PN/PS ratio	1729:1746	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	3	47	theme	tannery	714:720	arg1	influent					722:729	real tannery influent	709:729	real tannery influent	709:729	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	6	48	theme	PS	1448:1449	arg1	salinity					1489:1496	higher salinity	1482:1496	higher salinity of the mixed liquid	1482:1516	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	48	theme	PS	1448:1449	arg1	attachment					1541:1550	weakened cell-to-cell attachment	1519:1550	weakened cell-to-cell attachment	1519:1550	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	48	theme	PS	1448:1449	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	48	theme	PS	1448:1449	arg1	ratio					1475:1479	comparably lower PN/PS ratio	1452:1479	comparably lower PN/PS ratio	1452:1479	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	49	theme	PN	1441:1442	arg1	salinity					1489:1496	higher salinity	1482:1496	higher salinity of the mixed liquid	1482:1516	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	49	theme	PN	1441:1442	arg1	attachment					1541:1550	weakened cell-to-cell attachment	1519:1550	weakened cell-to-cell attachment	1519:1550	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	49	theme	PN	1441:1442	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	49	theme	PN	1441:1442	arg1	ratio					1475:1479	comparably lower PN/PS ratio	1452:1479	comparably lower PN/PS ratio	1452:1479	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	7	50	theme	nitrogen	1759:1766	arg1	ratio					1768:1772	carbon to nitrogen ratio	1749:1772	carbon to nitrogen ratio of feed influent	1749:1789	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	7	50	theme	nitrogen	1759:1766	arg1	ratio					1742:1746	sludge PN/PS ratio	1729:1746	sludge PN/PS ratio	1729:1746	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	6	51	theme	liquid	1511:1516	arg1	salinity					1489:1496	higher salinity	1482:1496	higher salinity of the mixed liquid	1482:1516	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	51	theme	liquid	1511:1516	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	1	52	theme	advanced	191:198	arg1	treatment					211:219	advanced biological treatment for industrial wastewater could be considered a poorly explored area	191:288	advanced biological treatment for industrial wastewater could be considered a poorly explored area	191:288	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	7	53	theme	carbon	1749:1754	arg1	ratio					1768:1772	carbon to nitrogen ratio	1749:1772	carbon to nitrogen ratio of feed influent	1749:1789	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	7	53	theme	carbon	1749:1754	arg1	ratio					1742:1746	sludge PN/PS ratio	1729:1746	sludge PN/PS ratio	1729:1746	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	6	54	theme	favoring	1237:1244	arg1	range					1246:1250	favoring range	1237:1250	favoring range of SRT (24 days) for MANODOX implementation	1237:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	55	theme	in-situ	1321:1327	arg1	minimization					1342:1353	an outstanding in-situ sludge yield minimization	1306:1353	an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment	1306:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	1	56	theme	industrial	225:234	arg1	wastewater					236:245	industrial wastewater	225:245	industrial wastewater	225:245	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	4	57	from	sludge	901:906	arg1	PN					847:848	PN	847:848	PN	847:848	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	4	57	from	sludge	901:906	arg1	polysaccharides					852:866	polysaccharides	852:866	polysaccharides (PS)	852:871	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	4	57	from	sludge	901:906	arg1	protein					838:844	protein	838:844	protein (PN)	838:849	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	4	57	from	sludge	901:906	arg1	contents					883:890	humic contents	877:890	humic contents from the sludge	877:906	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	4	57	from	sludge	901:906	arg1	PS					869:870	PS	869:870	PS	869:870	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	6	58	theme	operational	1204:1214	arg1	range					1246:1250	favoring range	1237:1250	favoring range of SRT (24 days) for MANODOX implementation	1237:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	58	theme	operational	1204:1214	arg1	strategies					1216:1225	adapted operational strategies	1196:1225	adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation	1196:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	8	59	theme	operational	1942:1952	arg1	tactics					1954:1960	aforementioned operational tactics	1927:1960	aforementioned operational tactics	1927:1960	The observations confirmed that the sludge yield minimization from MANODOX like systems could be effectively controlled by maintaining aforementioned operational tactics.
35051823	7	60	from	decline	1673:1679	arg1	aggregation					1689:1699	floc aggregation	1684:1699	floc aggregation	1684:1699	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	6	61	theme	parallel	1568:1575	arg1	run					1577:1579	a parallel run	1566:1579	a parallel run identical aerobic treatment	1566:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	5	62	theme	sludge	1024:1029	arg1	index					1038:1042	sludge volume index	1024:1042	sludge volume index	1024:1042	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	3	63	theme	degradation	620:630	arg1	mechanism					632:640	EPS degradation mechanism	616:640	EPS degradation mechanism	616:640	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	7	64	theme	PN/PS	1736:1740	arg1	ratio					1768:1772	carbon to nitrogen ratio	1749:1772	carbon to nitrogen ratio of feed influent	1749:1789	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	7	64	theme	PN/PS	1736:1740	arg1	ratio					1742:1746	sludge PN/PS ratio	1729:1746	sludge PN/PS ratio	1729:1746	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	4	65	theme	humic	877:881	arg1	contents					883:890	humic contents	877:890	humic contents from the sludge	877:906	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	6	66	theme	strict	1174:1179	arg1	maintenance					1181:1191	The strict maintenance	1170:1191	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation	1170:1294	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	1	67	theme	sludge	160:165	arg1	minimization					173:184	The sludge yield minimization	156:184	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area	156:288	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	0	68	theme	upgraded	93:100	arg1	performance					78:88	in-situ sludge minimization performance	50:88	in-situ sludge minimization performance of upgraded	50:100	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	5	69	theme	sludge	1112:1117	arg1	SRT					1135:1137	SRT	1135:1137	SRT	1135:1137	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	69	theme	sludge	1112:1117	arg1	time					1129:1132	hydrodynamic sludge retention time	1099:1132	hydrodynamic sludge retention time (SRT) of 08-40 days	1099:1152	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	6	70	theme	weakened	1519:1526	arg1	attachment					1541:1550	weakened cell-to-cell attachment	1519:1550	weakened cell-to-cell attachment	1519:1550	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	70	theme	weakened	1519:1526	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	0	71	theme	industrial	133:142	arg1	wastewater					144:153	industrial wastewater	133:153	industrial wastewater	133:153	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	7	72	from	hydrophobicity	1640:1653	arg1	aggregation					1689:1699	floc aggregation	1684:1699	floc aggregation	1684:1699	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	0	73	theme	sludge	112:117	arg1	treatment					119:127	sludge treatment	112:127	sludge treatment for industrial wastewater	112:153	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	4	74	theme	heat	761:764	arg1	method					777:782	a modified heat extraction method	750:782	a modified heat extraction method	750:782	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	6	75	theme	yield	1336:1340	arg1	minimization					1342:1353	an outstanding in-situ sludge yield minimization	1306:1353	an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment	1306:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	5	76	theme	corresponding	1075:1087	arg1	change					1089:1094	corresponding change	1075:1094	corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days	1075:1152	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	7	77	theme	floc	1684:1687	arg1	aggregation					1689:1699	floc aggregation	1684:1699	floc aggregation	1684:1699	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	2	78	theme	tannery	516:522	arg1	wastewater					524:533	real tannery wastewater	511:533	real tannery wastewater	511:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	2	79	theme	substances	412:421	arg1	profile					429:435	the extracellular polymeric substances (EPS) profile	384:435	the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater	384:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	5	80	from	change	1089:1094	arg1	variation					944:952	the variation	940:952	the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days	940:1152	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	80	from	change	1089:1094	arg1	SRT					1135:1137	SRT	1135:1137	SRT	1135:1137	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	80	from	change	1089:1094	arg1	time					1129:1132	hydrodynamic sludge retention time	1099:1132	hydrodynamic sludge retention time (SRT) of 08-40 days	1099:1152	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	2	81	theme	activated	460:468	arg1	treatment					477:485	an upgraded activated sludge treatment	448:485	an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater	448:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	8	82	from	MANODOX	1859:1865	arg1	minimization					1841:1852	the sludge yield minimization	1824:1852	the sludge yield minimization from MANODOX like systems	1824:1878	The observations confirmed that the sludge yield minimization from MANODOX like systems could be effectively controlled by maintaining aforementioned operational tactics.
35051823	2	83	theme	extracellular	388:400	arg1	substances					412:421	extracellular polymeric substances	388:421	the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater	384:533	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	2	83	theme	extracellular	388:400	arg1	EPS					424:426	EPS	424:426	EPS	424:426	Up to best of the knowledge, the extracellular polymeric substances (EPS) profile underlying an upgraded activated sludge treatment (as MANODOX system) for real tannery wastewater has not been addressed in a desired manner.
35051823	0	84	theme	polymeric	24:32	arg1	compositions					34:45	extracellular polymeric compositions	10:45	extracellular polymeric compositions	10:45	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	5	85	theme	days	1149:1152	arg1	SRT					1135:1137	SRT	1135:1137	SRT	1135:1137	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	85	theme	days	1149:1152	arg1	time					1129:1132	hydrodynamic sludge retention time	1099:1132	hydrodynamic sludge retention time (SRT) of 08-40 days	1099:1152	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	6	86	theme	identical	1581:1589	arg1	treatment					1599:1607	a parallel run identical aerobic treatment	1566:1607	a parallel run identical aerobic treatment	1566:1607	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	5	87	theme	present	917:923	arg1	investigation					925:937	the present investigation	913:937	the present investigation	913:937	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	3	88	theme	morphology	651:660	arg1	elucidation					601:611	the elucidation	597:611	the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent	597:729	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	7	89	theme	improved	1631:1638	arg1	hydrophobicity					1640:1653	improved hydrophobicity	1631:1653	improved hydrophobicity	1631:1653	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	0	90	theme	sludge	58:63	arg1	performance					78:88	in-situ sludge minimization performance	50:88	in-situ sludge minimization performance of upgraded	50:100	Effect of extracellular polymeric compositions on in-situ sludge minimization performance of upgraded activated sludge treatment for industrial wastewater.
35051823	1	91	theme	scientific	333:342	arg1	community					344:352	the scientific community	329:352	the scientific community	329:352	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
35051823	5	92	theme	sludge	1001:1006	arg1	hydrophobicity					1008:1021	sludge hydrophobicity	1001:1021	sludge hydrophobicity	1001:1021	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	4	93	theme	fractions	823:831	arg1	estimation					805:814	the estimation	801:814	the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge	801:906	For this purpose, a modified heat extraction method was followed for the estimation of EPS fractions like protein (PN), polysaccharides (PS) and humic contents from the sludge.
35051823	5	94	theme	floc	957:960	arg1	microbiota					1061:1070	facultative microbiota	1049:1070	facultative microbiota	1049:1070	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	94	theme	floc	957:960	arg1	index					1038:1042	sludge volume index	1024:1042	sludge volume index	1024:1042	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	94	theme	floc	957:960	arg1	characteristics					962:976	floc characteristics	957:976	floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota	957:1070	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	94	theme	floc	957:960	arg1	ratio					994:998	PN/PS ratio	988:998	PN/PS ratio	988:998	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	94	theme	floc	957:960	arg1	hydrophobicity					1008:1021	sludge hydrophobicity	1001:1021	sludge hydrophobicity	1001:1021	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	7	95	dep	ratio	1768:1772	arg1	to					1756:1757	to	1756:1757	to	1756:1757	Here, the reason for improved hydrophobicity and corresponding decline in floc aggregation was attributed to change in sludge PN/PS ratio, carbon to nitrogen ratio of feed influent.
35051823	3	96	theme	MANODOX	673:679	arg1	system					681:686	MANODOX system	673:686	MANODOX system for the treatment of real tannery influent	673:729	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	6	97	theme	lower	1463:1467	arg1	accumulation					1425:1436	accumulation	1425:1436	accumulation of PN and PS	1425:1449	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	6	97	theme	lower	1463:1467	arg1	ratio					1475:1479	comparably lower PN/PS ratio	1452:1479	comparably lower PN/PS ratio	1452:1479	The strict maintenance of adapted operational strategies including favoring range of SRT (24 days) for MANODOX implementation succeeded an outstanding in-situ sludge yield minimization lowered up to 0.39 gMLSS/gTCOD that attributed to three times lowered accumulation of PN and PS, comparably lower PN/PS ratio, higher salinity of the mixed liquid, weakened cell-to-cell attachment compared with a parallel run identical aerobic treatment.
35051823	3	98	theme	real	709:712	arg1	influent					722:729	real tannery influent	709:729	real tannery influent	709:729	This study covers the elucidation of EPS degradation mechanism and floc morphology underlying MANODOX system for the treatment of real tannery influent.
35051823	5	99	from	variation	944:952	arg1	microbiota					1061:1070	facultative microbiota	1049:1070	facultative microbiota	1049:1070	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	99	from	variation	944:952	arg1	index					1038:1042	sludge volume index	1024:1042	sludge volume index	1024:1042	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	99	from	variation	944:952	arg1	characteristics					962:976	floc characteristics	957:976	floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota	957:1070	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	99	from	variation	944:952	arg1	ratio					994:998	PN/PS ratio	988:998	PN/PS ratio	988:998	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	5	99	from	variation	944:952	arg1	hydrophobicity					1008:1021	sludge hydrophobicity	1001:1021	sludge hydrophobicity	1001:1021	For the present investigation, the variation in floc characteristics including PN/PS ratio, sludge hydrophobicity, sludge volume index, and facultative microbiota at corresponding change in hydrodynamic sludge retention time (SRT) of 08-40 days was emphasized.
35051823	1	100	theme	explored	276:283	arg1	area					285:288	a poorly explored area	267:288	a poorly explored area	267:288	The sludge yield minimization from advanced biological treatment for industrial wastewater could be considered a poorly explored area, therefore, seeks serious attention of the scientific community.
37350810	2	0	with	patients	377:384	arg1	AD					391:392	AD	391:392	AD	391:392	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	2	0	with	patients	377:384	arg1	impairment					412:421	mild cognitive impairment	397:421	mild cognitive impairment	397:421	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	11	1	theme	Genes	1383:1387	arg1	KEGG					1402:1405	KEGG	1402:1405	KEGG	1402:1405	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	11	1	theme	Genes	1383:1387	arg1	Genomes					1393:1399	Genomes	1393:1399	Genomes (KEGG)	1393:1406	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	8	2	theme	acid	1070:1073	arg1	levels					1028:1033	the levels	1024:1033	the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET)	1024:1098	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	12	3	theme	Bacteroides	1587:1597	arg1	abundance					1574:1582	The abundance	1570:1582	The abundance of Bacteroides and Faecalibacterium	1570:1618	The abundance of Bacteroides and Faecalibacterium was negatively correlated with the content of ET, and positively correlated with the scores of MMSE and MoCA.
37350810	10	4	contain	had	1294:1296	arg2	levels					1334:1339	higher levels	1327:1339	higher levels of DAO, DA, and ET	1327:1358	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	4	contain	had	1294:1296	arg1	patients					1245:1252	patients	1245:1252	patients with cognitive impairments (AD and MCI)	1245:1292	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	4	contain	had	1294:1296	arg2	abundance					1304:1312	lower abundance	1298:1312	lower abundance of Dorea	1298:1321	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	6	5	theme	rRNA	790:793	arg1	sequencing					811:820	rRNA high-throughput sequencing	790:820	rRNA high-throughput sequencing	790:820	The composition of gut microbiome was examined by 16S rRNA high-throughput sequencing.
37350810	12	6	theme	Faecalibacterium	1603:1618	arg1	abundance					1574:1582	The abundance	1570:1582	The abundance of Bacteroides and Faecalibacterium	1570:1618	The abundance of Bacteroides and Faecalibacterium was negatively correlated with the content of ET, and positively correlated with the scores of MMSE and MoCA.
37350810	1	7	theme	cognitive	170:178	arg1	impairments					180:190	cognitive impairments	170:190	cognitive impairments	170:190	Background Gut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer's disease (AD).
37350810	8	8	theme	endotoxin	1085:1093	arg1	levels					1028:1033	the levels	1024:1033	the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET)	1024:1098	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	2	9	theme	gut	292:294	arg1	composition					306:316	gut microbial composition	292:316	gut microbial composition	292:316	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	4	10	theme	Mini-Mental	574:584	arg1	Examination					592:602	Mini-Mental State Examination	574:602	Mini-Mental State Examination (MMSE)	574:609	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	4	10	theme	Mini-Mental	574:584	arg1	MMSE					605:608	MMSE	605:608	MMSE	605:608	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	9	11	theme	high-sensitivity	1111:1126	arg1	hs-CRP					1148:1153	hs-CRP	1148:1153	hs-CRP	1148:1153	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	9	11	theme	high-sensitivity	1111:1126	arg1	protein					1139:1145	serum high-sensitivity C-reactive protein	1105:1145	The serum high-sensitivity C-reactive protein (hs-CRP) level	1101:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	7	12	theme	functional	934:943	arg1	transfer					945:952	functional transfer	934:952	functional transfer of gut microbiota	934:970	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	15	13	theme	intestinal	1991:2000	arg1	dysfunction					2010:2020	intestinal barrier dysfunction	1991:2020	intestinal barrier dysfunction	1991:2020	Conclusion Cognitive impairments might be associated with gut microbial dysbiosis and intestinal barrier dysfunction.
37350810	4	14	theme	Assessment	635:644	arg1	MoCA					653:656	MoCA	653:656	MoCA	653:656	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	4	14	theme	Assessment	635:644	arg1	Scale					646:650	Montreal Cognitive Assessment Scale	616:650	Montreal Cognitive Assessment Scale (MoCA)	616:657	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	9	15	theme	protein	1139:1145	arg1	level					1156:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level	1101:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level	1101:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	1	16	theme	Gut-brain	123:131	arg1	axis					133:136	Background Gut-brain axis	112:136	Background Gut-brain axis	112:136	Background Gut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer's disease (AD).
37350810	7	17	theme	gut	957:959	arg1	microbiota					961:970	gut microbiota	957:970	gut microbiota	957:970	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	10	18	theme	Dorea	1317:1321	arg1	levels					1334:1339	higher levels	1327:1339	higher levels of DAO, DA, and ET	1327:1358	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	18	theme	Dorea	1317:1321	arg1	abundance					1304:1312	lower abundance	1298:1312	lower abundance of Dorea	1298:1321	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	4	19	theme	Montreal	616:623	arg1	MoCA					653:656	MoCA	653:656	MoCA	653:656	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	4	19	theme	Montreal	616:623	arg1	Scale					646:650	Montreal Cognitive Assessment Scale	616:650	Montreal Cognitive Assessment Scale (MoCA)	616:657	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	2	20	with	cases	454:458	arg1	AD					391:392	AD	391:392	AD	391:392	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	2	20	with	cases	454:458	arg1	impairment					412:421	mild cognitive impairment	397:421	mild cognitive impairment	397:421	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	7	21	used	used	918:921	arg2	Investigation					836:848	Phylogenetic Investigation	823:848	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt)	823:912	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	0	22	dep	study	105:109	arg1	function					38:45	intestinal barrier function	19:45	intestinal barrier function	19:45	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	0	22	dep	study	105:109	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota	0:13	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	5	23	theme	Functional	660:669	arg1	ability					671:677	Functional ability	660:677	Functional ability	660:677	Functional ability was assessed using Activity of Daily Living Scale (ADL).
37350810	8	24	theme	Gut	973:975	arg1	dysfunction					985:995	Gut barrier dysfunction	973:995	Gut barrier dysfunction	973:995	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	3	25	dep	Methods	461:467	arg1	total					471:475	A total	469:475	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC)	461:518	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited.
37350810	10	26	theme	higher	1327:1332	arg1	levels					1334:1339	higher levels	1327:1339	higher levels of DAO, DA, and ET	1327:1358	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	0	27	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota	0:13	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	15	28	theme	microbial	1967:1975	arg1	dysbiosis					1977:1985	gut microbial dysbiosis	1963:1985	gut microbial dysbiosis	1963:1985	Conclusion Cognitive impairments might be associated with gut microbial dysbiosis and intestinal barrier dysfunction.
37350810	2	29	theme	NC	450:451	arg1	cases					454:458	normal control (NC) cases	434:458	normal control (NC) cases	434:458	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	11	30	theme	related	1529:1535	arg1	ones					1524:1527	the ones	1520:1527	the ones related to membrane transport	1520:1557	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	2	31	theme	cognitive	402:410	arg1	impairment					412:421	mild cognitive impairment	397:421	mild cognitive impairment	397:421	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	0	32	theme	barrier	30:36	arg1	function					38:45	intestinal barrier function	19:45	intestinal barrier function	19:45	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	6	33	theme	microbiome	759:768	arg1	composition					740:750	The composition	736:750	The composition of gut microbiome	736:768	The composition of gut microbiome was examined by 16S rRNA high-throughput sequencing.
37350810	14	34	theme	significant	1787:1797	arg1	correlation					1808:1818	A significant negative correlation	1785:1818	A significant negative correlation	1785:1818	A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.
37350810	2	35	theme	control	441:447	arg1	cases					454:458	normal control (NC) cases	434:458	normal control (NC) cases	434:458	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	8	36	dep	endotoxin	1085:1093	arg1	ET					1096:1097	ET	1096:1097	ET	1096:1097	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	5	37	theme	Living	716:721	arg1	ADL					730:732	ADL	730:732	ADL	730:732	Functional ability was assessed using Activity of Daily Living Scale (ADL).
37350810	5	37	theme	Living	716:721	arg1	Scale					723:727	Daily Living Scale	710:727	Daily Living Scale (ADL)	710:733	Functional ability was assessed using Activity of Daily Living Scale (ADL).
37350810	1	38	theme	Background	112:121	arg1	axis					133:136	Background Gut-brain axis	112:136	Background Gut-brain axis	112:136	Background Gut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer's disease (AD).
37350810	15	39	theme	gut	1963:1965	arg1	dysbiosis					1977:1985	gut microbial dysbiosis	1963:1985	gut microbial dysbiosis	1963:1985	Conclusion Cognitive impairments might be associated with gut microbial dysbiosis and intestinal barrier dysfunction.
37350810	11	40	dep	Encyclopedia	1367:1378	arg1	results					1408:1414	results	1408:1414	results	1408:1414	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	10	41	theme	ET	1357:1358	arg1	levels					1334:1339	higher levels	1327:1339	higher levels of DAO, DA, and ET	1327:1358	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	41	theme	ET	1357:1358	arg1	abundance					1304:1312	lower abundance	1298:1312	lower abundance of Dorea	1298:1321	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	2	42	theme	barrier	330:336	arg1	function					338:345	intestinal barrier function	319:345	intestinal barrier function	319:345	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	8	43	theme	diamine	1038:1044	arg1	DAO					1055:1057	DAO	1055:1057	DAO	1055:1057	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	8	43	theme	diamine	1038:1044	arg1	oxidase					1046:1052	diamine oxidase	1038:1052	diamine oxidase (DAO)	1038:1058	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	11	44	theme	membrane	1540:1547	arg1	transport					1549:1557	membrane transport	1540:1557	membrane transport	1540:1557	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	11	45	theme	glycan	1452:1457	arg1	biosynthesis					1459:1470	glycan biosynthesis	1452:1470	glycan biosynthesis	1452:1470	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	4	46	theme	Cognitive	536:544	arg1	function					546:553	Cognitive function	536:553	Cognitive function	536:553	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	11	47	theme	related	1441:1447	arg1	pathways					1432:1439	the pathways	1428:1439	the pathways related to glycan biosynthesis and metabolism	1428:1485	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	2	48	theme	systemic	352:359	arg1	inflammation					361:372	systemic inflammation	352:372	systemic inflammation	352:372	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	14	49	theme	cognitive	1885:1893	arg1	function					1895:1902	cognitive function	1885:1902	cognitive function	1885:1902	A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.
37350810	8	50	theme	D-lactic	1061:1068	arg1	acid					1070:1073	D-lactic acid	1061:1073	D-lactic acid (DA)	1061:1078	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	8	50	theme	D-lactic	1061:1068	arg1	DA					1076:1077	DA	1076:1077	DA	1076:1077	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	6	51	theme	high-throughput	795:809	arg1	sequencing					811:820	rRNA high-throughput sequencing	790:820	rRNA high-throughput sequencing	790:820	The composition of gut microbiome was examined by 16S rRNA high-throughput sequencing.
37350810	10	52	theme	cognitive	1259:1267	arg1	MCI					1289:1291	MCI	1289:1291	MCI	1289:1291	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	52	theme	cognitive	1259:1267	arg1	AD					1282:1283	AD	1282:1283	AD	1282:1283	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	52	theme	cognitive	1259:1267	arg1	impairments					1269:1279	cognitive impairments	1259:1279	cognitive impairments (AD and MCI)	1259:1292	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	3	53	theme	subjects	484:491	arg1	total					471:475	A total	469:475	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC)	461:518	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited.
37350810	4	54	theme	State	586:590	arg1	Examination					592:602	Mini-Mental State Examination	574:602	Mini-Mental State Examination (MMSE)	574:609	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	4	54	theme	State	586:590	arg1	MMSE					605:608	MMSE	605:608	MMSE	605:608	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	11	55	theme	MCI	1500:1502	arg1	patients					1504:1511	MCI patients	1500:1511	MCI patients	1500:1511	Kyoto Encyclopedia of Genes and Genomes (KEGG) results showed that the pathways related to glycan biosynthesis and metabolism increased in MCI patients, while the ones related to membrane transport decreased.
37350810	14	56	theme	function	1895:1902	arg1	severity					1845:1852	the severity	1841:1852	the severity of gut barrier dysfunction and cognitive function	1841:1902	A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.
37350810	2	57	theme	microbial	296:304	arg1	composition					306:316	gut microbial composition	292:316	gut microbial composition	292:316	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	7	58	theme	States	897:902	arg1	Reconstruction					868:881	Reconstruction	868:881	Reconstruction of Unobserved States (PICRUSt)	868:912	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	1	59	theme	various	195:201	arg1	diseases					203:210	various diseases	195:210	various diseases including Alzheimer's disease (AD)	195:245	Background Gut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer's disease (AD).
37350810	1	59	theme	various	195:201	arg1	disease					234:240	Alzheimer's disease	222:240	Alzheimer's disease (AD)	222:245	Background Gut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer's disease (AD).
37350810	9	60	theme	serum	1105:1109	arg1	hs-CRP					1148:1153	hs-CRP	1148:1153	hs-CRP	1148:1153	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	9	60	theme	serum	1105:1109	arg1	protein					1139:1145	serum high-sensitivity C-reactive protein	1105:1145	The serum high-sensitivity C-reactive protein (hs-CRP) level	1101:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	7	61	theme	microbiota	961:970	arg1	transfer					945:952	functional transfer	934:952	functional transfer of gut microbiota	934:970	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	9	62	theme	C-reactive	1128:1137	arg1	hs-CRP					1148:1153	hs-CRP	1148:1153	hs-CRP	1148:1153	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	9	62	theme	C-reactive	1128:1137	arg1	protein					1139:1145	serum high-sensitivity C-reactive protein	1105:1145	The serum high-sensitivity C-reactive protein (hs-CRP) level	1101:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	15	63	theme	Cognitive	1916:1924	arg1	impairments					1926:1936	Conclusion Cognitive impairments	1905:1936	Conclusion Cognitive impairments	1905:1936	Conclusion Cognitive impairments might be associated with gut microbial dysbiosis and intestinal barrier dysfunction.
37350810	14	64	theme	dysfunction	1869:1879	arg1	severity					1845:1852	the severity	1841:1852	the severity of gut barrier dysfunction and cognitive function	1841:1902	A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.
37350810	3	65	dep	subjects	484:491	arg1	45 AD					494:498	45 AD	494:498	45 AD	494:498	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited.
37350810	3	65	dep	subjects	484:491	arg1	MCI					504:506	38 MCI	501:506	38 MCI	501:506	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited.
37350810	3	65	dep	subjects	484:491	arg1	subjects					484:491	118 subjects	480:491	118 subjects (45 AD, 38 MCI, and 35 NC)	480:518	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited.
37350810	3	65	dep	subjects	484:491	arg1	NC					516:517	35 NC	513:517	35 NC	513:517	Methods A total of 118 subjects (45 AD, 38 MCI, and 35 NC) were recruited.
37350810	10	66	with	patients	1245:1252	arg1	MCI					1289:1291	MCI	1289:1291	MCI	1289:1291	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	66	with	patients	1245:1252	arg1	AD					1282:1283	AD	1282:1283	AD	1282:1283	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	66	with	patients	1245:1252	arg1	impairments					1269:1279	cognitive impairments	1259:1279	cognitive impairments (AD and MCI)	1259:1292	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	4	67	theme	Cognitive	625:633	arg1	MoCA					653:656	MoCA	653:656	MoCA	653:656	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	4	67	theme	Cognitive	625:633	arg1	Scale					646:650	Montreal Cognitive Assessment Scale	616:650	Montreal Cognitive Assessment Scale (MoCA)	616:657	Cognitive function was assessed using Mini-Mental State Examination (MMSE), and Montreal Cognitive Assessment Scale (MoCA).
37350810	13	68	theme	hs-CRP	1734:1739	arg1	levels					1741:1746	The hs-CRP levels	1730:1746	The hs-CRP levels	1730:1746	The hs-CRP levels were similar among the three groups.
37350810	13	68	theme	hs-CRP	1734:1739	arg1	similar					1753:1759	similar	1753:1759	similar	1753:1759	The hs-CRP levels were similar among the three groups.
37350810	0	69	theme	cognitive	64:72	arg1	impairments					74:84	cognitive impairments	64:84	cognitive impairments	64:84	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	15	70	theme	barrier	2002:2008	arg1	dysfunction					2010:2020	intestinal barrier dysfunction	1991:2020	intestinal barrier dysfunction	1991:2020	Conclusion Cognitive impairments might be associated with gut microbial dysbiosis and intestinal barrier dysfunction.
37350810	2	71	from	differences	277:287	arg1	MCI					424:426	MCI	424:426	MCI	424:426	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	2	71	from	differences	277:287	arg1	composition					306:316	gut microbial composition	292:316	gut microbial composition	292:316	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	2	71	from	differences	277:287	arg1	inflammation					361:372	systemic inflammation	352:372	systemic inflammation	352:372	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	2	71	from	differences	277:287	arg1	cases					454:458	normal control (NC) cases	434:458	normal control (NC) cases	434:458	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	2	71	from	differences	277:287	arg1	function					338:345	intestinal barrier function	319:345	intestinal barrier function	319:345	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	2	71	from	differences	277:287	arg1	patients					377:384	patients	377:384	patients with AD or mild cognitive impairment (MCI)	377:427	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	1	72	theme	important	152:160	arg1	role					162:165	an important role	149:165	an important role	149:165	Background Gut-brain axis might play an important role in cognitive impairments by various diseases including Alzheimer's disease (AD).
37350810	0	73	from	microbiota	4:13	arg1	subjects					50:57	subjects	50:57	subjects with cognitive impairments	50:84	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	0	74	theme	cross-sectional	89:103	arg1	study					105:109	a cross-sectional study	87:109	a cross-sectional study	87:109	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	14	75	theme	gut	1857:1859	arg1	dysfunction					1869:1879	gut barrier dysfunction	1857:1879	gut barrier dysfunction	1857:1879	A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.
37350810	10	76	theme	lower	1298:1302	arg1	abundance					1304:1312	lower abundance	1298:1312	lower abundance of Dorea	1298:1321	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	9	77	theme	systemic	1183:1190	arg1	inflammation					1192:1203	systemic inflammation	1183:1203	systemic inflammation	1183:1203	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	8	78	theme	barrier	977:983	arg1	dysfunction					985:995	Gut barrier dysfunction	973:995	Gut barrier dysfunction	973:995	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	7	79	theme	Unobserved	886:895	arg1	PICRUSt					905:911	PICRUSt	905:911	PICRUSt	905:911	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	7	79	theme	Unobserved	886:895	arg1	States					897:902	Unobserved States	886:902	Unobserved States (PICRUSt)	886:912	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	0	80	from	function	38:45	arg1	subjects					50:57	subjects	50:57	subjects with cognitive impairments	50:84	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	5	81	theme	Scale	723:727	arg1	Activity					698:705	Activity	698:705	Activity of Daily Living Scale (ADL)	698:733	Functional ability was assessed using Activity of Daily Living Scale (ADL).
37350810	12	82	theme	MoCA	1724:1727	arg1	scores					1705:1710	the scores	1701:1710	the scores of MMSE and MoCA	1701:1727	The abundance of Bacteroides and Faecalibacterium was negatively correlated with the content of ET, and positively correlated with the scores of MMSE and MoCA.
37350810	0	83	theme	intestinal	19:28	arg1	function					38:45	intestinal barrier function	19:45	intestinal barrier function	19:45	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	7	84	theme	Communities	853:863	arg1	Investigation					836:848	Phylogenetic Investigation	823:848	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt)	823:912	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	10	85	theme	DA	1349:1350	arg1	levels					1334:1339	higher levels	1327:1339	higher levels of DAO, DA, and ET	1327:1358	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	85	theme	DA	1349:1350	arg1	abundance					1304:1312	lower abundance	1298:1312	lower abundance of Dorea	1298:1321	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	12	86	theme	MMSE	1715:1718	arg1	scores					1705:1710	the scores	1701:1710	the scores of MMSE and MoCA	1701:1727	The abundance of Bacteroides and Faecalibacterium was negatively correlated with the content of ET, and positively correlated with the scores of MMSE and MoCA.
37350810	14	87	theme	negative	1799:1806	arg1	correlation					1808:1818	A significant negative correlation	1785:1818	A significant negative correlation	1785:1818	A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.
37350810	10	88	theme	DAO	1344:1346	arg1	levels					1334:1339	higher levels	1327:1339	higher levels of DAO, DA, and ET	1327:1358	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	88	theme	DAO	1344:1346	arg1	abundance					1304:1312	lower abundance	1298:1312	lower abundance of Dorea	1298:1321	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	89	theme	normal	1228:1233	arg1	controls					1235:1242	normal controls	1228:1242	normal controls	1228:1242	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	2	90	theme	mild	397:400	arg1	impairment					412:421	mild cognitive impairment	397:421	mild cognitive impairment	397:421	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	5	91	theme	Daily	710:714	arg1	ADL					730:732	ADL	730:732	ADL	730:732	Functional ability was assessed using Activity of Daily Living Scale (ADL).
37350810	5	91	theme	Daily	710:714	arg1	Scale					723:727	Daily Living Scale	710:727	Daily Living Scale (ADL)	710:733	Functional ability was assessed using Activity of Daily Living Scale (ADL).
37350810	6	92	theme	gut	755:757	arg1	microbiome					759:768	gut microbiome	755:768	gut microbiome	755:768	The composition of gut microbiome was examined by 16S rRNA high-throughput sequencing.
37350810	2	93	theme	normal	434:439	arg1	cases					454:458	normal control (NC) cases	434:458	normal control (NC) cases	434:458	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	9	94	used	used	1166:1169	arg2	level					1156:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level	1101:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level	1101:1160	The serum high-sensitivity C-reactive protein (hs-CRP) level was used to indicate systemic inflammation.
37350810	7	95	theme	Phylogenetic	823:834	arg1	Investigation					836:848	Phylogenetic Investigation	823:848	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt)	823:912	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predict functional transfer of gut microbiota.
37350810	8	96	theme	oxidase	1046:1052	arg1	levels					1028:1033	the levels	1024:1033	the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET)	1024:1098	Gut barrier dysfunction was evaluated by measuring the levels of diamine oxidase (DAO), D-lactic acid (DA), and endotoxin (ET).
37350810	12	97	theme	ET	1666:1667	arg1	content					1655:1661	the content	1651:1661	the content of ET	1651:1667	The abundance of Bacteroides and Faecalibacterium was negatively correlated with the content of ET, and positively correlated with the scores of MMSE and MoCA.
37350810	14	98	theme	barrier	1861:1867	arg1	dysfunction					1869:1879	gut barrier dysfunction	1857:1879	gut barrier dysfunction	1857:1879	A significant negative correlation was observed between the severity of gut barrier dysfunction and cognitive function.
37350810	0	99	with	subjects	50:57	arg1	impairments					74:84	cognitive impairments	64:84	cognitive impairments	64:84	Gut microbiota and intestinal barrier function in subjects with cognitive impairments: a cross-sectional study.
37350810	2	100	theme	intestinal	319:328	arg1	function					338:345	intestinal barrier function	319:345	intestinal barrier function	319:345	Objective To investigate the differences in gut microbial composition, intestinal barrier function, and systemic inflammation in patients with AD or mild cognitive impairment (MCI), and normal control (NC) cases.
37350810	10	101	dep	impairments	1269:1279	arg1	MCI					1289:1291	MCI	1289:1291	MCI	1289:1291	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	101	dep	impairments	1269:1279	arg1	AD					1282:1283	AD	1282:1283	AD	1282:1283	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
37350810	10	101	dep	impairments	1269:1279	arg1	impairments					1269:1279	cognitive impairments	1259:1279	cognitive impairments (AD and MCI)	1259:1292	Results Compared with normal controls, patients with cognitive impairments (AD and MCI) had lower abundance of Dorea and higher levels of DAO, DA, and ET.
35681522	0	0	theme	Oxygen	87:92	arg1	Species					94:100	Mitochondrial Reactive Oxygen Species	64:100	Mitochondrial Reactive Oxygen Species Level	64:106	Short O-GlcNAcase Is Targeted to the Mitochondria and Regulates Mitochondrial Reactive Oxygen Species Level.
35681522	5	1	from	cells	829:833	arg1	fractions					785:793	mitochondria-enriched fractions	763:793	mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts	763:873	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	3	2	theme	O-GlcNAcylates	465:478	arg1	proteins					480:487	O-GlcNAcylates proteins	465:487	O-GlcNAcylates proteins	465:487	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	0	3	theme	Reactive	78:85	arg1	Species					94:100	Mitochondrial Reactive Oxygen Species	64:100	Mitochondrial Reactive Oxygen Species Level	64:106	Short O-GlcNAcase Is Targeted to the Mitochondria and Regulates Mitochondrial Reactive Oxygen Species Level.
35681522	5	4	theme	embryonic	853:861	arg1	fibroblasts					863:873	mouse embryonic fibroblasts	847:873	mouse embryonic fibroblasts	847:873	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	2	5	attach	removal	346:352	arg2	O-GlcNAc					357:364	O-GlcNAc	357:364	O-GlcNAc	357:364	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	2	5	attach	removal	346:352	arg3	proteins					369:376	proteins	369:376	proteins	369:376	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	0	6	theme	Species	94:100	arg1	Level					102:106	Mitochondrial Reactive Oxygen Species Level	64:106	Mitochondrial Reactive Oxygen Species Level	64:106	Short O-GlcNAcase Is Targeted to the Mitochondria and Regulates Mitochondrial Reactive Oxygen Species Level.
35681522	2	7	from	attachment	331:340	arg1	proteins					369:376	proteins	369:376	proteins	369:376	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	5	8	theme	mouse	847:851	arg1	fibroblasts					863:873	mouse embryonic fibroblasts	847:873	mouse embryonic fibroblasts	847:873	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	9	9	theme	short-OGA	1472:1480	arg1	isoform					1482:1488	the short-OGA isoform	1468:1488	the short-OGA isoform	1468:1488	Together, our work reveals that the short-OGA isoform is targeted to the mitochondria where it regulates ROS homoeostasis.
35681522	3	10	theme	main	447:450	arg1	isoform					452:458	the main isoform	443:458	the main isoform that O-GlcNAcylates proteins in mitochondria	443:503	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	3	10	theme	main	447:450	arg1	OGT					411:413	a variant OGT	401:413	a variant OGT (mOGT)	401:420	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	5	11	theme	fractionation	682:694	arg1	experiments					696:706	cell fractionation experiments	677:706	cell fractionation experiments	677:706	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	2	12	attach	attachment	331:340	arg2	O-GlcNAc					357:364	O-GlcNAc	357:364	O-GlcNAc	357:364	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	2	12	attach	attachment	331:340	arg3	proteins					369:376	proteins	369:376	proteins	369:376	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	7	13	theme	O-GlcNAcylation	1073:1087	arg1	biosensor					1089:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	8	14	theme	ROS	1380:1382	arg1	levels					1384:1389	mitochondrial ROS levels	1366:1389	mitochondrial ROS levels	1366:1389	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	8	15	theme	short-OGA	1331:1339	arg1	overexpression					1341:1354	short-OGA overexpression	1331:1354	short-OGA overexpression	1331:1354	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	5	16	from	fibroblasts	863:873	arg1	fractions					785:793	mitochondria-enriched fractions	763:793	mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts	763:873	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	8	17	theme	mitochondrial	1366:1378	arg1	levels					1384:1389	mitochondrial ROS levels	1366:1389	mitochondrial ROS levels	1366:1389	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	2	18	from	removal	346:352	arg1	proteins					369:376	proteins	369:376	proteins	369:376	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	2	19	theme	O-GlcNAc	274:281	arg1	OGT					269:271	OGT	269:271	OGT (O-GlcNAc transferase)	269:294	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	2	19	theme	O-GlcNAc	274:281	arg1	transferase					283:293	O-GlcNAc transferase	274:293	O-GlcNAc transferase	274:293	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	4	20	theme	long	610:613	arg1	isoforms					615:622	short and long isoforms	600:622	short and long isoforms	600:622	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	4	20	theme	long	610:613	arg1	variants					583:590	Two splice variants	572:590	Two splice variants of OGA (short and long isoforms)	572:623	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	9	21	theme	ROS	1541:1543	arg1	homoeostasis					1545:1556	ROS homoeostasis	1541:1556	ROS homoeostasis	1541:1556	Together, our work reveals that the short-OGA isoform is targeted to the mitochondria where it regulates ROS homoeostasis.
35681522	7	22	theme	-based	1052:1057	arg1	biosensor					1089:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	7	23	theme	significant	1217:1227	arg1	effect					1229:1234	no significant effect	1214:1234	no significant effect	1214:1234	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	1	24	theme	cytosolic	203:211	arg1	proteins					241:248	cytosolic, nuclear, and mitochondrial proteins	203:248	cytosolic, nuclear, and mitochondrial proteins	203:248	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	0	25	theme	Short	0:4	arg1	O-GlcNAcase					6:16	Short O-GlcNAcase	0:16	Short O-GlcNAcase	0:16	Short O-GlcNAcase Is Targeted to the Mitochondria and Regulates Mitochondrial Reactive Oxygen Species Level.
35681522	7	26	theme	mitochondrial	1059:1071	arg1	biosensor					1089:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	3	27	from	isoform	452:458	arg1	mitochondria					492:503	mitochondria	492:503	mitochondria	492:503	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	5	28	theme	mitochondria-enriched	763:783	arg1	fractions					785:793	mitochondria-enriched fractions	763:793	mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts	763:873	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	3	29	theme	mitochondrial	526:538	arg1	OGA					540:542	a mitochondrial OGA	524:542	a mitochondrial OGA	524:542	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	1	30	theme	nuclear	214:220	arg1	proteins					241:248	cytosolic, nuclear, and mitochondrial proteins	203:248	cytosolic, nuclear, and mitochondrial proteins	203:248	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	7	31	contain	had	1210:1212	arg2	effect					1229:1234	no significant effect	1214:1234	no significant effect	1214:1234	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	7	31	contain	had	1210:1212	arg1	long-OGA					1201:1208	long-OGA	1201:1208	long-OGA	1201:1208	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	6	32	theme	GFP-tagged	932:941	arg1	short-OGA					943:951	GFP-tagged short-OGA	932:951	GFP-tagged short-OGA	932:951	Moreover, fluorescent microscopy imaging confirmed that GFP-tagged short-OGA is addressed to mitochondria.
35681522	8	33	theme	encoded	1264:1270	arg1	probes					1310:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	3	34	theme	OGA	540:542	arg1	identification					506:519	identification	506:519	identification of a mitochondrial OGA	506:542	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	4	35	theme	OGA	595:597	arg1	isoforms					615:622	short and long isoforms	600:622	short and long isoforms	600:622	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	4	35	theme	OGA	595:597	arg1	variants					583:590	Two splice variants	572:590	Two splice variants of OGA (short and long isoforms)	572:623	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	7	36	theme	short-OGA	1132:1140	arg1	co-transfection					1113:1127	co-transfection	1113:1127	co-transfection of short-OGA	1113:1140	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	7	37	theme	Transfer	1037:1044	arg1	biosensor					1089:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	8	38	theme	chemical	1275:1282	arg1	probes					1310:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	3	39	theme	variant	403:409	arg1	mOGT					416:419	mOGT	416:419	mOGT	416:419	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	3	39	theme	variant	403:409	arg1	isoform					452:458	the main isoform	443:458	the main isoform that O-GlcNAcylates proteins in mitochondria	443:503	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	3	39	theme	variant	403:409	arg1	OGT					411:413	a variant OGT	401:413	a variant OGT (mOGT)	401:420	Whereas a variant OGT (mOGT) has been proposed as the main isoform that O-GlcNAcylates proteins in mitochondria, identification of a mitochondrial OGA has not been performed yet.
35681522	5	40	theme	RAW	819:821	arg1	cells					829:833	RAW 264.7 cells	819:833	RAW 264.7 cells	819:833	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	1	41	theme	mitochondrial	227:239	arg1	proteins					241:248	cytosolic, nuclear, and mitochondrial proteins	203:248	cytosolic, nuclear, and mitochondrial proteins	203:248	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	8	42	contain	has	1409:1411	arg2	effect					1428:1433	no significant effect	1413:1433	no significant effect	1413:1433	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	8	42	contain	has	1409:1411	arg1	long-OGA					1400:1407	long-OGA	1400:1407	long-OGA	1400:1407	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	7	43	theme	Resonance	1020:1028	arg1	BRET					1047:1050	BRET	1047:1050	BRET	1047:1050	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	7	43	theme	Resonance	1020:1028	arg1	Transfer					1037:1044	a Bioluminescence Resonance Energy Transfer	1002:1044	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	8	44	theme	mitochondrial	1296:1308	arg1	probes					1310:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	6	45	theme	microscopy	898:907	arg1	imaging					909:915	fluorescent microscopy imaging	886:915	fluorescent microscopy imaging	886:915	Moreover, fluorescent microscopy imaging confirmed that GFP-tagged short-OGA is addressed to mitochondria.
35681522	7	46	theme	Energy	1030:1035	arg1	BRET					1047:1050	BRET	1047:1050	BRET	1047:1050	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	7	46	theme	Energy	1030:1035	arg1	Transfer					1037:1044	a Bioluminescence Resonance Energy Transfer	1002:1044	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	8	47	theme	significant	1416:1426	arg1	effect					1428:1433	no significant effect	1413:1433	no significant effect	1413:1433	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	8	48	theme	fluorescent	1284:1294	arg1	probes					1310:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	genetically encoded or chemical fluorescent mitochondrial probes	1252:1315	Finally, using genetically encoded or chemical fluorescent mitochondrial probes, we show that short-OGA overexpression increases mitochondrial ROS levels, whereas long-OGA has no significant effect.
35681522	6	49	theme	fluorescent	886:896	arg1	imaging					909:915	fluorescent microscopy imaging	886:915	fluorescent microscopy imaging	886:915	Moreover, fluorescent microscopy imaging confirmed that GFP-tagged short-OGA is addressed to mitochondria.
35681522	5	50	from	cells	809:813	arg1	fractions					785:793	mitochondria-enriched fractions	763:793	mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts	763:873	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	1	51	theme	reversible	130:139	arg1	modification					160:171	a reversible post-translational modification	128:171	a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins	128:248	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	1	51	theme	reversible	130:139	arg1	O-GlcNAcylation					109:123	O-GlcNAcylation	109:123	O-GlcNAcylation	109:123	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	2	52	dep	attachment	331:340	arg1	the					327:329	the	327:329	the	327:329	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	1	53	theme	proteins	241:248	arg1	regulation					189:198	the regulation	185:198	the regulation of cytosolic, nuclear, and mitochondrial proteins	185:248	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	7	54	theme	Bioluminescence	1004:1018	arg1	BRET					1047:1050	BRET	1047:1050	BRET	1047:1050	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	7	54	theme	Bioluminescence	1004:1018	arg1	Transfer					1037:1044	a Bioluminescence Resonance Energy Transfer	1002:1044	a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor	1002:1097	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	1	55	theme	post-translational	141:158	arg1	modification					160:171	a reversible post-translational modification	128:171	a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins	128:248	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	1	55	theme	post-translational	141:158	arg1	O-GlcNAcylation					109:123	O-GlcNAcylation	109:123	O-GlcNAcylation	109:123	O-GlcNAcylation is a reversible post-translational modification involved in the regulation of cytosolic, nuclear, and mitochondrial proteins.
35681522	0	56	theme	Mitochondrial	64:76	arg1	Species					94:100	Mitochondrial Reactive Oxygen Species	64:100	Mitochondrial Reactive Oxygen Species Level	64:106	Short O-GlcNAcase Is Targeted to the Mitochondria and Regulates Mitochondrial Reactive Oxygen Species Level.
35681522	4	57	theme	short	600:604	arg1	isoforms					615:622	short and long isoforms	600:622	short and long isoforms	600:622	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	4	57	theme	short	600:604	arg1	variants					583:590	Two splice variants	572:590	Two splice variants of OGA (short and long isoforms)	572:623	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	5	58	theme	HEK-293T	800:807	arg1	cells					809:813	HEK-293T cells	800:813	HEK-293T cells	800:813	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
35681522	2	59	theme	O-GlcNAc	357:364	arg1	removal					346:352	removal	346:352	removal	346:352	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	2	59	theme	O-GlcNAc	357:364	arg1	attachment					331:340	attachment	331:340	attachment	331:340	Only two enzymes, OGT (O-GlcNAc transferase) and OGA (O-GlcNAcase), control the attachment and removal of O-GlcNAc on proteins, respectively.
35681522	7	60	theme	biosensor	1182:1190	arg1	O-GlcNAcylation					1159:1173	O-GlcNAcylation	1159:1173	O-GlcNAcylation of the biosensor	1159:1190	In addition, using a Bioluminescence Resonance Energy Transfer (BRET)-based mitochondrial O-GlcNAcylation biosensor, we show that co-transfection of short-OGA markedly reduced O-GlcNAcylation of the biosensor, whereas long-OGA had no significant effect.
35681522	4	61	theme	splice	576:581	arg1	isoforms					615:622	short and long isoforms	600:622	short and long isoforms	600:622	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	4	61	theme	splice	576:581	arg1	variants					583:590	Two splice variants	572:590	Two splice variants of OGA (short and long isoforms)	572:623	Two splice variants of OGA (short and long isoforms) have been described previously.
35681522	5	62	theme	cell	677:680	arg1	experiments					696:706	cell fractionation experiments	677:706	cell fractionation experiments	677:706	In this work, using cell fractionation experiments, we show that short-OGA is preferentially recovered in mitochondria-enriched fractions from HEK-293T cells and RAW 264.7 cells, as well as mouse embryonic fibroblasts.
36005290	7	0	theme	simulation	1530:1539	arg1	GROMACS					1560:1566	GROMACS	1560:1566	GROMACS	1560:1566	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	0	theme	simulation	1530:1539	arg1	programs					1541:1548	MD simulation programs	1527:1548	MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1527:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	0	theme	simulation	1530:1539	arg1	NAMD					1577:1580	NAMD	1577:1580	NAMD	1577:1580	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	0	theme	simulation	1530:1539	arg1	CHARMM					1587:1592	CHARMM	1587:1592	CHARMM	1587:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	0	theme	simulation	1530:1539	arg1	OPENMM					1569:1574	OPENMM	1569:1574	OPENMM	1569:1574	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	1	1	theme	accurate	250:257	arg1	field					298:302	an accurate and reliable molecular mechanics force field	247:302	an accurate and reliable molecular mechanics force field	247:302	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	2	2	theme	folding	602:608	arg1	cooperativity					610:622	peptide folding cooperativity	594:622	peptide folding cooperativity	594:622	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	7	3	theme	Drude	1476:1480	arg1	field					1500:1504	Drude polarizable force field	1476:1504	Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1476:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	1	4	theme	reliable	263:270	arg1	field					298:302	an accurate and reliable molecular mechanics force field	247:302	an accurate and reliable molecular mechanics force field	247:302	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	7	5	theme	field	1500:1504	arg1	remainder					1463:1471	the remainder	1459:1471	the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1459:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	2	6	theme	hairpin	629:635	arg1	structures					637:646	RNA hairpin structures	625:646	RNA hairpin structures	625:646	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	3	7	theme	important	777:785	arg1	molecules					787:795	many pharmaceutically important molecules	755:795	many pharmaceutically important molecules	755:795	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	6	8	theme	model	1272:1276	arg1	Validation					1254:1263	Validation	1254:1263	Validation of the model	1254:1276	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	2	9	theme	Drude	309:313	arg1	field					333:337	The Drude polarizable force field	305:337	The Drude polarizable force field	305:337	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	1	10	theme	molecular	185:193	arg1	systems					195:201	complex molecular systems	177:201	complex molecular systems	177:201	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	7	11	gly	glycoprotein	1389:1400	arg1	glycoprotein					1389:1400	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	2	12	theme	additive	562:569	arg1	field					577:581	the CHARMM additive force field	551:581	the CHARMM additive force field	551:581	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	3	13	theme	many	755:758	arg1	molecules					787:795	many pharmaceutically important molecules	755:795	many pharmaceutically important molecules	755:795	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	6	14	theme	O-linked	1356:1363	arg1	glycosylations					1365:1378	O-linked glycosylations	1356:1378	O-linked glycosylations	1356:1378	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	5	15	theme	NMR	1200:1202	arg1	data					1224:1227	NMR solution J-coupling data	1200:1227	NMR solution J-coupling data for model glycopeptides	1200:1251	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	4	16	gly	glycoproteins	1053:1065	arg1	glycoproteins					1053:1065	glycoproteins	1053:1065	glycoproteins	1053:1065	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	4	16	gly	glycoproteins	1053:1065	arg1	N-					1083:1084	N-	1083:1084	N-	1083:1084	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	4	16	gly	glycoproteins	1053:1065	arg1	species					1099:1105	O-linked species	1090:1105	O-linked species	1090:1105	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	1	17	theme	Molecular	116:124	arg1	simulations					134:144	Molecular dynamic simulations	116:144	Molecular dynamic simulations	116:144	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	1	17	theme	Molecular	116:124	arg1	tool					163:166	an effective tool	150:166	an effective tool	150:166	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	7	18	theme	Prepper	1632:1638	arg1	module					1640:1645	the Drude Prepper module	1622:1645	the Drude Prepper module in the CHARMM-GUI	1622:1663	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	19	theme	carbohydrate	1402:1413	arg1	field					1421:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	20	used	used	1434:1437	arg2	field					1421:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	21	theme	new	1385:1387	arg1	field					1421:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	3	22	theme	polarizable	814:824	arg1	field					832:836	an accurate polarizable force field	802:836	an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field	802:927	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	22	theme	polarizable	814:824	arg1	essential					932:940	essential	932:940	essential	932:940	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	0	23	theme	Field	58:62	arg1	Extension					0:8	Extension	0:8	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.	0:114	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.
36005290	6	24	gly	glycoproteins	1321:1333	arg1	glycoproteins					1321:1333	four glycoproteins	1316:1333	four glycoproteins	1316:1333	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	3	25	theme	force	917:921	arg1	field					923:927	the force field	913:927	the force field	913:927	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	5	26	theme	solution	1204:1211	arg1	data					1224:1227	NMR solution J-coupling data	1200:1227	NMR solution J-coupling data for model glycopeptides	1200:1251	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	5	27	theme	dihedral	1146:1153	arg1	terms					1155:1159	the dihedral terms	1142:1159	the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides	1142:1251	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	3	28	dep	protein	878:884	arg1	aspect					903:908	aspect	903:908	aspect	903:908	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	28	dep	protein	878:884	arg1	the					874:876	the	874:876	the	874:876	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	2	29	theme	base	657:660	arg1	flipping					662:669	DNA base flipping	653:669	DNA base flipping	653:669	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	0	30	theme	O-Linked	74:81	arg1	Glycopeptides					83:95	Glycopeptides	83:95	Glycopeptides	83:95	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.
36005290	7	31	theme	polarizable	1482:1492	arg1	field					1500:1504	Drude polarizable force field	1476:1504	Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1476:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	2	32	theme	polarization	394:405	arg1	treatment					370:378	the explicit treatment	357:378	the explicit treatment of electronic polarization	357:405	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	4	33	theme	force	1038:1042	arg1	field					1044:1048	the Drude polarizable force field	1016:1048	the Drude polarizable force field	1016:1048	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	5	34	theme	J-coupling	1213:1222	arg1	data					1224:1227	NMR solution J-coupling data	1200:1227	NMR solution J-coupling data for model glycopeptides	1200:1251	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	7	35	with	conjunction	1442:1452	arg1	remainder					1463:1471	the remainder	1459:1471	the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1459:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	1	36	theme	molecular	272:280	arg1	field					298:302	an accurate and reliable molecular mechanics force field	247:302	an accurate and reliable molecular mechanics force field	247:302	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	2	37	theme	efficient	428:436	arg1	fashion					438:444	a computationally efficient fashion	410:444	a computationally efficient fashion	410:444	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	1	38	theme	force	292:296	arg1	field					298:302	an accurate and reliable molecular mechanics force field	247:302	an accurate and reliable molecular mechanics force field	247:302	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	2	39	theme	experimental	475:486	arg1	properties					488:497	experimental properties	475:497	experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping	475:669	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	0	40	theme	Classical	24:32	arg1	Field					58:62	the CHARMM Classical Drude Polarizable Force Field	13:62	the CHARMM Classical Drude Polarizable Force Field	13:62	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.
36005290	4	41	theme	Drude	1020:1024	arg1	field					1044:1048	the Drude polarizable force field	1016:1048	the Drude polarizable force field	1016:1048	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	0	42	theme	Polarizable	40:50	arg1	Field					58:62	the CHARMM Classical Drude Polarizable Force Field	13:62	the CHARMM Classical Drude Polarizable Force Field	13:62	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.
36005290	5	43	theme	reweighting	1169:1179	arg1	protocol					1181:1188	a reweighting protocol	1167:1188	a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides	1167:1251	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	4	44	attach	present	992:998	arg2	we					989:990	we	989:990	we	989:990	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	4	44	attach	present	992:998	arg1	work					983:986	this work	978:986	this work	978:986	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	3	45	theme	essential	690:698	arg1	Glycoproteins					672:684	Glycoproteins	672:684	Glycoproteins	672:684	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	45	theme	essential	690:698	arg1	components					700:709	essential components	690:709	essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules	690:795	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	6	46	with	glycopeptides	1298:1310	arg1	N-					1349:1350	multiple N-	1340:1350	multiple N-	1340:1350	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	6	46	with	glycopeptides	1298:1310	arg1	glycosylations					1365:1378	O-linked glycosylations	1356:1378	O-linked glycosylations	1356:1378	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	7	47	theme	programs	1541:1548	arg1	NAMD					1577:1580	NAMD	1577:1580	NAMD	1577:1580	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	47	theme	programs	1541:1548	arg1	programs					1541:1548	MD simulation programs	1527:1548	MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1527:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	47	theme	programs	1541:1548	arg1	GROMACS					1560:1566	GROMACS	1560:1566	GROMACS	1560:1566	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	47	theme	programs	1541:1548	arg1	CHARMM					1587:1592	CHARMM	1587:1592	CHARMM	1587:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	47	theme	programs	1541:1548	arg1	variety					1516:1522	a variety	1514:1522	a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1514:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	47	theme	programs	1541:1548	arg1	OPENMM					1569:1574	OPENMM	1569:1574	OPENMM	1569:1574	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	2	48	theme	explicit	361:368	arg1	treatment					370:378	the explicit treatment	357:378	the explicit treatment of electronic polarization	357:405	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	7	49	theme	MD	1527:1528	arg1	GROMACS					1560:1566	GROMACS	1560:1566	GROMACS	1560:1566	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	49	theme	MD	1527:1528	arg1	programs					1541:1548	MD simulation programs	1527:1548	MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1527:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	49	theme	MD	1527:1528	arg1	NAMD					1577:1580	NAMD	1577:1580	NAMD	1577:1580	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	49	theme	MD	1527:1528	arg1	CHARMM					1587:1592	CHARMM	1587:1592	CHARMM	1587:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	49	theme	MD	1527:1528	arg1	OPENMM					1569:1574	OPENMM	1569:1574	OPENMM	1569:1574	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	3	50	theme	vaccines	729:736	arg1	Glycoproteins					672:684	Glycoproteins	672:684	Glycoproteins	672:684	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	50	theme	vaccines	729:736	arg1	components					700:709	essential components	690:709	essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules	690:795	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	4	51	link	O-linked	1090:1097	arg1	species					1099:1105	O-linked species	1090:1105	O-linked species	1090:1105	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	5	52	theme	model	1233:1237	arg1	glycopeptides					1239:1251	model glycopeptides	1233:1251	model glycopeptides	1233:1251	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	2	53	theme	peptide	594:600	arg1	cooperativity					610:622	peptide folding cooperativity	594:622	peptide folding cooperativity	594:622	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	6	54	gly	glycopeptides	1298:1310	arg2	glycopeptides					1298:1310	eight model glycopeptides	1286:1310	eight model glycopeptides	1286:1310	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	6	55	theme	model	1292:1296	arg1	glycopeptides					1298:1310	eight model glycopeptides	1286:1310	eight model glycopeptides	1286:1310	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	7	56	theme	force	1494:1498	arg1	field					1500:1504	Drude polarizable force field	1476:1504	Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM	1476:1592	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	3	57	theme	antibodies	739:748	arg1	Glycoproteins					672:684	Glycoproteins	672:684	Glycoproteins	672:684	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	57	theme	antibodies	739:748	arg1	components					700:709	essential components	690:709	essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules	690:795	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	2	58	theme	polarizable	315:325	arg1	field					333:337	The Drude polarizable force field	305:337	The Drude polarizable force field	305:337	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	1	59	theme	complex	177:183	arg1	systems					195:201	complex molecular systems	177:201	complex molecular systems	177:201	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	2	60	theme	RNA	625:627	arg1	structures					637:646	RNA hairpin structures	625:646	RNA hairpin structures	625:646	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	3	61	theme	molecules	787:795	arg1	Glycoproteins					672:684	Glycoproteins	672:684	Glycoproteins	672:684	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	61	theme	molecules	787:795	arg1	components					700:709	essential components	690:709	essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules	690:795	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	2	62	theme	CHARMM	555:560	arg1	field					577:581	the CHARMM additive force field	551:581	the CHARMM additive force field	551:581	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	3	63	theme	accurate	805:812	arg1	field					832:836	an accurate polarizable force field	802:836	an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field	802:927	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	63	theme	accurate	805:812	arg1	essential					932:940	essential	932:940	essential	932:940	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	7	64	theme	Drude	1626:1630	arg1	module					1640:1645	the Drude Prepper module	1622:1645	the Drude Prepper module in the CHARMM-GUI	1622:1663	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	6	65	theme	multiple	1340:1347	arg1	N-					1349:1350	multiple N-	1340:1350	multiple N-	1340:1350	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	1	66	theme	dynamic	126:132	arg1	simulations					134:144	Molecular dynamic simulations	116:144	Molecular dynamic simulations	116:144	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	1	66	theme	dynamic	126:132	arg1	tool					163:166	an effective tool	150:166	an effective tool	150:166	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	2	67	theme	force	571:575	arg1	field					577:581	the CHARMM additive force field	551:581	the CHARMM additive force field	551:581	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	7	68	theme	force	1415:1419	arg1	field					1421:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	7	69	theme	glycoprotein	1389:1400	arg1	field					1421:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field	1381:1425	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	3	70	theme	force	826:830	arg1	field					832:836	an accurate polarizable force field	802:836	an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field	802:927	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	70	theme	force	826:830	arg1	essential					932:940	essential	932:940	essential	932:940	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	1	71	theme	effective	153:161	arg1	simulations					134:144	Molecular dynamic simulations	116:144	Molecular dynamic simulations	116:144	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	1	71	theme	effective	153:161	arg1	tool					163:166	an effective tool	150:166	an effective tool	150:166	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	3	72	theme	field	923:927	arg1	carbohydrate					890:901	carbohydrate	890:901	carbohydrate	890:901	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	3	72	theme	field	923:927	arg1	protein					878:884	protein	878:884	protein	878:884	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	7	73	from	module	1640:1645	arg1	CHARMM-GUI					1654:1663	the CHARMM-GUI	1650:1663	the CHARMM-GUI	1650:1663	The new glycoprotein carbohydrate force field can be used in conjunction with the remainder of Drude polarizable force field through a variety of MD simulation programs including GROMACS, OPENMM, NAMD, and CHARMM and may be accessed through the Drude Prepper module in the CHARMM-GUI.
36005290	2	74	theme	DNA	653:655	arg1	flipping					662:669	DNA base flipping	653:669	DNA base flipping	653:669	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	5	75	theme	Parameter	1108:1116	arg1	optimization					1118:1129	Parameter optimization	1108:1129	Parameter optimization	1108:1129	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	2	76	theme	electronic	383:392	arg1	polarization					394:405	electronic polarization	383:405	electronic polarization	383:405	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	4	77	theme	polarizable	1026:1036	arg1	field					1044:1048	the Drude polarizable force field	1016:1048	the Drude polarizable force field	1016:1048	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	5	78	gly	glycopeptides	1239:1251	arg2	glycopeptides					1239:1251	model glycopeptides	1233:1251	model glycopeptides	1233:1251	Parameter optimization focused on the dihedral terms using a reweighting protocol targeting NMR solution J-coupling data for model glycopeptides.
36005290	0	79	theme	CHARMM	17:22	arg1	Field					58:62	the CHARMM Classical Drude Polarizable Force Field	13:62	the CHARMM Classical Drude Polarizable Force Field	13:62	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.
36005290	1	80	theme	mechanics	282:290	arg1	field					298:302	an accurate and reliable molecular mechanics force field	247:302	an accurate and reliable molecular mechanics force field	247:302	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	3	81	theme	systems	966:972	arg1	types					957:961	these types	951:961	these types of systems	951:972	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36005290	4	82	theme	field	1044:1048	arg1	extension					1003:1011	an extension	1000:1011	an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species	1000:1105	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	0	83	theme	Drude	34:38	arg1	Field					58:62	the CHARMM Classical Drude Polarizable Force Field	13:62	the CHARMM Classical Drude Polarizable Force Field	13:62	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.
36005290	1	84	theme	field	298:302	arg1	availability					231:242	the availability	227:242	the availability of an accurate and reliable molecular mechanics force field	227:302	Molecular dynamic simulations are an effective tool to study complex molecular systems and are contingent upon the availability of an accurate and reliable molecular mechanics force field.
36005290	0	85	theme	Force	52:56	arg1	Field					58:62	the CHARMM Classical Drude Polarizable Force Field	13:62	the CHARMM Classical Drude Polarizable Force Field	13:62	Extension of the CHARMM Classical Drude Polarizable Force Field to N- and O-Linked Glycopeptides and Glycoproteins.
36005290	6	86	link	O-linked	1356:1363	arg1	glycosylations					1365:1378	O-linked glycosylations	1356:1378	O-linked glycosylations	1356:1378	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	2	87	theme	force	327:331	arg1	field					333:337	The Drude polarizable force field	305:337	The Drude polarizable force field	305:337	The Drude polarizable force field, which allows for the explicit treatment of electronic polarization in a computationally efficient fashion, has been shown to reproduce experimental properties that were difficult or impossible to reproduce with the CHARMM additive force field, including peptide folding cooperativity, RNA hairpin structures, and DNA base flipping.
36005290	6	88	with	glycoproteins	1321:1333	arg1	N-					1349:1350	multiple N-	1340:1350	multiple N-	1340:1350	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	6	88	with	glycoproteins	1321:1333	arg1	glycosylations					1365:1378	O-linked glycosylations	1356:1378	O-linked glycosylations	1356:1378	Validation of the model include eight model glycopeptides and four glycoproteins with multiple N- and O-linked glycosylations.
36005290	4	89	theme	O-linked	1090:1097	arg1	species					1099:1105	O-linked species	1090:1105	O-linked species	1090:1105	In this work, we present an extension of the Drude polarizable force field to glycoproteins, including both N- and O-linked species.
36005290	3	90	theme	glycoconjugate	714:727	arg1	vaccines					729:736	glycoconjugate vaccines	714:736	glycoconjugate vaccines	714:736	Glycoproteins are essential components of glycoconjugate vaccines, antibodies, and many pharmaceutically important molecules, and an accurate polarizable force field that includes compatibility between the protein and carbohydrate aspect of the force field is essential to study these types of systems.
36922975	5	0	theme	neutralizing	1205:1216	arg1	antibodies					1218:1227	anti-RVA neutralizing antibodies	1196:1227	anti-RVA neutralizing antibodies	1196:1227	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	6	1	theme	anti-inflammatory	1393:1409	arg1	bacterium					1411:1419	this anti-inflammatory bacterium	1388:1419	this anti-inflammatory bacterium	1388:1419	DP8α Tregs specifically recognize the human symbiont Faecalibacterium prausnitzii, which strongly supports their induction by this anti-inflammatory bacterium.
36922975	4	2	theme	young	801:805	arg1	adults					815:820	young healthy adults	801:820	young healthy adults	801:820	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	8	3	theme	microbiota	1600:1609	arg1	cells					1644:1648	the microbiota and microbiota-induced DP8α Treg cells	1596:1648	the microbiota and microbiota-induced DP8α Treg cells	1596:1648	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	3	theme	microbiota	1600:1609	arg1	gastroenteritis					1579:1593	RVA gastroenteritis	1575:1593	RVA gastroenteritis	1575:1593	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	4	theme	DP8α	1634:1637	arg1	Treg					1639:1642	microbiota-induced DP8α Treg	1615:1642	microbiota-induced DP8α Treg	1615:1642	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	5	theme	FUT2	1544:1547	arg1	genotype					1549:1556	the FUT2 genotype	1540:1556	the FUT2 genotype	1540:1556	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	6	theme	microbiota-induced	1615:1632	arg1	Treg					1639:1642	microbiota-induced DP8α Treg	1615:1642	microbiota-induced DP8α Treg	1615:1642	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	7	theme	lymphocytes	1744:1754	arg1	induction/expansion					1713:1731	an induction/expansion	1710:1731	an induction/expansion of these T lymphocytes	1710:1754	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	5	8	contain	had	1192:1194	arg2	antibodies					1218:1227	anti-RVA neutralizing antibodies	1196:1227	anti-RVA neutralizing antibodies	1196:1227	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	8	contain	had	1192:1194	arg1	individuals					1176:1186	individuals	1176:1186	individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs	1176:1259	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	8	contain	had	1192:1194	arg2	titers					1237:1242	high titers	1232:1242	high titers of anti-RVA IgAs	1232:1259	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	7	9	theme	prausnitzii	1443:1453	arg1	proportion					1426:1435	The proportion	1422:1435	The proportion of F. prausnitzii in feces	1422:1462	The proportion of F. prausnitzii in feces was also associated with the FUT2 wild-type allele.
36922975	6	10	theme	Faecalibacterium	1315:1330	arg1	prausnitzii					1332:1342	the human symbiont Faecalibacterium prausnitzii	1296:1342	the human symbiont Faecalibacterium prausnitzii	1296:1342	DP8α Tregs specifically recognize the human symbiont Faecalibacterium prausnitzii, which strongly supports their induction by this anti-inflammatory bacterium.
36922975	3	11	theme	autoimmune	656:665	arg1	diseases					667:674	several inflammatory or autoimmune diseases	632:674	diseases	667:674	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	4	12	theme	wild-type	842:850	arg1	allele					857:862	the wild-type FUT2 allele	838:862	the wild-type FUT2 allele	838:862	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	1	13	theme	life-threatening	301:316	arg1	gastroenteritis					318:332	life-threatening gastroenteritis	301:332	life-threatening gastroenteritis	301:332	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	6	14	theme	DP8α	1262:1265	arg1	Tregs					1267:1271	DP8α Tregs	1262:1271	DP8α Tregs	1262:1271	DP8α Tregs specifically recognize the human symbiont Faecalibacterium prausnitzii, which strongly supports their induction by this anti-inflammatory bacterium.
36922975	3	15	from	influence	717:725	arg1	composition					741:751	microbiota composition	730:751	microbiota composition	730:751	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	8	16	theme	Treg	1639:1642	arg1	cells					1644:1648	the microbiota and microbiota-induced DP8α Treg cells	1596:1648	the microbiota and microbiota-induced DP8α Treg cells	1596:1648	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	16	theme	Treg	1639:1642	arg1	gastroenteritis					1579:1593	RVA gastroenteritis	1575:1593	RVA gastroenteritis	1575:1593	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	7	17	theme	wild-type	1498:1506	arg1	allele					1508:1513	the FUT2 wild-type allele	1489:1513	the FUT2 wild-type allele	1489:1513	The proportion of F. prausnitzii in feces was also associated with the FUT2 wild-type allele.
36922975	3	18	theme	gastroenteritis	558:572	arg1	susceptibility					574:587	viral gastroenteritis susceptibility	552:587	viral gastroenteritis susceptibility	552:587	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	1	19	theme	attachment	205:214	arg1	factors					216:222	attachment factors	205:222	attachment factors	205:222	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	4	20	theme	antibodies	941:950	arg1	presence					888:895	the presence	884:895	the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA	884:963	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	0	21	theme	regulatory	19:28	arg1	cells					32:36	Microbiota-induced regulatory T cells	0:36	Microbiota-induced regulatory T cells	0:36	Microbiota-induced regulatory T cells associate with FUT2-dependent susceptibility to rotavirus gastroenteritis.
36922975	4	22	theme	IgA	961:963	arg1	presence					888:895	the presence	884:895	the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA	884:963	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	5	23	theme	microbiota-induced	1092:1109	arg1	cells					1124:1128	gut microbiota-induced regulatory T cells	1088:1128	gut microbiota-induced regulatory T cells (Tregs)	1088:1136	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	23	theme	microbiota-induced	1092:1109	arg1	Tregs					1154:1158	so-called DP8α Tregs	1139:1158	so-called DP8α Tregs	1139:1158	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	23	theme	microbiota-induced	1092:1109	arg1	Tregs					1131:1135	Tregs	1131:1135	Tregs	1131:1135	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	3	24	theme	unclear	684:690	arg1	reasons					692:698	yet unclear reasons	680:698	yet unclear reasons	680:698	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	0	25	theme	Microbiota-induced	0:17	arg1	cells					32:36	Microbiota-induced regulatory T cells	0:36	Microbiota-induced regulatory T cells	0:36	Microbiota-induced regulatory T cells associate with FUT2-dependent susceptibility to rotavirus gastroenteritis.
36922975	5	26	theme	IgAs	1256:1259	arg1	antibodies					1218:1227	anti-RVA neutralizing antibodies	1196:1227	anti-RVA neutralizing antibodies	1196:1227	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	26	theme	IgAs	1256:1259	arg1	titers					1237:1242	high titers	1232:1242	high titers of anti-RVA IgAs	1232:1259	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	4	27	theme	serum	955:959	arg1	IgA					961:963	serum IgA	955:963	serum IgA	955:963	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	1	28	theme	FUT2	117:120	arg1	α1,2fucosyltransferase					122:143	The FUT2 α1,2fucosyltransferase	113:143	The FUT2 α1,2fucosyltransferase	113:143	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	1	29	theme	several	227:233	arg1	rotaviruses					272:282	rotaviruses	272:282	rotaviruses	272:282	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	1	29	theme	several	227:233	arg1	pathogens					235:243	several pathogens	227:243	several pathogens	227:243	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	1	29	theme	several	227:233	arg1	noroviruses					256:266	noroviruses	256:266	noroviruses	256:266	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	1	30	theme	young	337:341	arg1	children					343:350	young children	337:350	young children	337:350	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	4	31	theme	gastroenteritis	1014:1028	arg1	risk					1002:1005	the risk	998:1005	the risk of RVA gastroenteritis	998:1028	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	2	32	theme	genetic	358:364	arg1	polymorphisms					366:378	FUT2 genetic polymorphisms	353:378	FUT2 genetic polymorphisms impairing fucosylation	353:401	FUT2 genetic polymorphisms impairing fucosylation are strongly associated with resistance to dominant strains of both noroviruses and rotaviruses.
36922975	7	33	theme	FUT2	1493:1496	arg1	allele					1508:1513	the FUT2 wild-type allele	1489:1513	the FUT2 wild-type allele	1489:1513	The proportion of F. prausnitzii in feces was also associated with the FUT2 wild-type allele.
36922975	3	34	theme	viral	552:556	arg1	susceptibility					574:587	viral gastroenteritis susceptibility	552:587	viral gastroenteritis susceptibility	552:587	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	0	35	theme	T	30:30	arg1	cells					32:36	Microbiota-induced regulatory T cells	0:36	Microbiota-induced regulatory T cells	0:36	Microbiota-induced regulatory T cells associate with FUT2-dependent susceptibility to rotavirus gastroenteritis.
36922975	8	36	theme	immune	1680:1685	arg1	response					1687:1694	the anti-RVA immune response	1667:1694	the anti-RVA immune response	1667:1694	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	4	37	theme	RVA	1010:1012	arg1	gastroenteritis					1014:1028	RVA gastroenteritis	1010:1028	RVA gastroenteritis	1010:1028	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	2	38	theme	FUT2	353:356	arg1	polymorphisms					366:378	FUT2 genetic polymorphisms	353:378	FUT2 genetic polymorphisms impairing fucosylation	353:401	FUT2 genetic polymorphisms impairing fucosylation are strongly associated with resistance to dominant strains of both noroviruses and rotaviruses.
36922975	1	39	theme	fucosylated	177:187	arg1	glycans					189:195	fucosylated glycans	177:195	fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children	177:350	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	8	40	theme	anti-RVA	1671:1678	arg1	response					1687:1694	the anti-RVA immune response	1667:1694	the anti-RVA immune response	1667:1694	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	41	theme	inflammatory	1835:1846	arg1	diseases					1848:1855	inflammatory diseases	1835:1855	inflammatory diseases	1835:1855	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	5	42	theme	T	1122:1122	arg1	cells					1124:1128	gut microbiota-induced regulatory T cells	1088:1128	gut microbiota-induced regulatory T cells (Tregs)	1088:1136	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	42	theme	T	1122:1122	arg1	Tregs					1154:1158	so-called DP8α Tregs	1139:1158	so-called DP8α Tregs	1139:1158	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	42	theme	T	1122:1122	arg1	Tregs					1131:1135	Tregs	1131:1135	Tregs	1131:1135	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	7	43	theme	F.	1440:1441	arg1	prausnitzii					1443:1453	F. prausnitzii	1440:1453	F. prausnitzii	1440:1453	The proportion of F. prausnitzii in feces was also associated with the FUT2 wild-type allele.
36922975	2	44	theme	rotaviruses	487:497	arg1	strains					455:461	dominant strains	446:461	dominant strains of both noroviruses and rotaviruses	446:497	FUT2 genetic polymorphisms impairing fucosylation are strongly associated with resistance to dominant strains of both noroviruses and rotaviruses.
36922975	2	45	theme	noroviruses	471:481	arg1	strains					455:461	dominant strains	446:461	dominant strains of both noroviruses and rotaviruses	446:497	FUT2 genetic polymorphisms impairing fucosylation are strongly associated with resistance to dominant strains of both noroviruses and rotaviruses.
36922975	5	46	theme	high	1232:1235	arg1	titers					1237:1242	high titers	1232:1242	high titers of anti-RVA IgAs	1232:1259	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	3	47	theme	FUT2	712:715	arg1	influence					717:725	a FUT2 influence	710:725	a FUT2 influence on microbiota composition	710:751	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	8	48	theme	immunological	1783:1795	arg1	state					1797:1801	a balanced immunological state	1772:1801	a balanced immunological state that confers protection against inflammatory diseases	1772:1855	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	3	49	theme	wild-type	519:527	arg1	protective					613:622	protective	613:622	protective	613:622	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	3	49	theme	wild-type	519:527	arg1	allele					529:534	the wild-type allele	515:534	the wild-type allele associated with viral gastroenteritis susceptibility inversely	515:597	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	4	50	theme	neutralizing	928:939	arg1	antibodies					941:950	neutralizing antibodies	928:950	neutralizing antibodies	928:950	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	4	51	theme	adults	815:820	arg1	cohort					791:796	a cohort	789:796	a cohort of young healthy adults	789:820	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	0	52	theme	FUT2-dependent	53:66	arg1	susceptibility					68:81	FUT2-dependent susceptibility	53:81	FUT2-dependent susceptibility to rotavirus gastroenteritis	53:110	Microbiota-induced regulatory T cells associate with FUT2-dependent susceptibility to rotavirus gastroenteritis.
36922975	1	53	gly	fucosylated	177:187	arg1	glycans					189:195	fucosylated glycans	177:195	fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children	177:350	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
36922975	8	54	theme	gastroenteritis	1579:1593	arg1	risk					1567:1570	the risk	1563:1570	the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells	1563:1648	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	6	55	theme	symbiont	1306:1313	arg1	prausnitzii					1332:1342	the human symbiont Faecalibacterium prausnitzii	1296:1342	the human symbiont Faecalibacterium prausnitzii	1296:1342	DP8α Tregs specifically recognize the human symbiont Faecalibacterium prausnitzii, which strongly supports their induction by this anti-inflammatory bacterium.
36922975	4	56	theme	healthy	807:813	arg1	adults					815:820	young healthy adults	801:820	young healthy adults	801:820	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	4	57	with	association	981:991	arg1	risk					1002:1005	the risk	998:1005	the risk of RVA gastroenteritis	998:1028	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	5	58	theme	anti-RVA	1247:1254	arg1	IgAs					1256:1259	anti-RVA IgAs	1247:1259	anti-RVA IgAs	1247:1259	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	59	theme	cells	1124:1128	arg1	frequency					1075:1083	the frequency	1071:1083	the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs	1071:1158	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	6	60	theme	human	1300:1304	arg1	prausnitzii					1332:1342	the human symbiont Faecalibacterium prausnitzii	1296:1342	the human symbiont Faecalibacterium prausnitzii	1296:1342	DP8α Tregs specifically recognize the human symbiont Faecalibacterium prausnitzii, which strongly supports their induction by this anti-inflammatory bacterium.
36922975	3	61	theme	microbiota	730:739	arg1	composition					741:751	microbiota composition	730:751	microbiota composition	730:751	Interestingly, the wild-type allele associated with viral gastroenteritis susceptibility inversely appears to be protective against several inflammatory or autoimmune diseases for yet unclear reasons, although a FUT2 influence on microbiota composition has been observed.
36922975	4	62	theme	antibodies	909:918	arg1	presence					888:895	the presence	884:895	the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA	884:963	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	4	63	theme	FUT2	852:855	arg1	allele					857:862	the wild-type FUT2 allele	838:862	the wild-type FUT2 allele	838:862	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	7	64	from	proportion	1426:1435	arg1	feces					1458:1462	feces	1458:1462	feces	1458:1462	The proportion of F. prausnitzii in feces was also associated with the FUT2 wild-type allele.
36922975	5	65	theme	so-called	1139:1147	arg1	cells					1124:1128	gut microbiota-induced regulatory T cells	1088:1128	gut microbiota-induced regulatory T cells (Tregs)	1088:1136	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	65	theme	so-called	1139:1147	arg1	Tregs					1154:1158	so-called DP8α Tregs	1139:1158	so-called DP8α Tregs	1139:1158	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	66	theme	gut	1088:1090	arg1	cells					1124:1128	gut microbiota-induced regulatory T cells	1088:1128	gut microbiota-induced regulatory T cells (Tregs)	1088:1136	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	66	theme	gut	1088:1090	arg1	Tregs					1154:1158	so-called DP8α Tregs	1139:1158	so-called DP8α Tregs	1139:1158	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	66	theme	gut	1088:1090	arg1	Tregs					1131:1135	Tregs	1131:1135	Tregs	1131:1135	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	8	67	theme	RVA	1575:1577	arg1	gastroenteritis					1579:1593	RVA gastroenteritis	1575:1593	RVA gastroenteritis	1575:1593	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	67	theme	RVA	1575:1577	arg1	cells					1644:1648	the microbiota and microbiota-induced DP8α Treg cells	1596:1648	the microbiota and microbiota-induced DP8α Treg cells	1596:1648	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	0	68	theme	rotavirus	86:94	arg1	gastroenteritis					96:110	rotavirus gastroenteritis	86:110	rotavirus gastroenteritis	86:110	Microbiota-induced regulatory T cells associate with FUT2-dependent susceptibility to rotavirus gastroenteritis.
36922975	5	69	theme	DP8α	1149:1152	arg1	cells					1124:1128	gut microbiota-induced regulatory T cells	1088:1128	gut microbiota-induced regulatory T cells (Tregs)	1088:1136	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	69	theme	DP8α	1149:1152	arg1	Tregs					1154:1158	so-called DP8α Tregs	1139:1158	so-called DP8α Tregs	1139:1158	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	4	70	theme	anti-RVA	900:907	arg1	antibodies					909:918	anti-RVA antibodies	900:918	anti-RVA antibodies	900:918	Here, we studied a cohort of young healthy adults and showed that the wild-type FUT2 allele was associated with the presence of anti-RVA antibodies, either neutralizing antibodies or serum IgA, confirming its association with the risk of RVA gastroenteritis.
36922975	2	71	theme	dominant	446:453	arg1	strains					455:461	dominant strains	446:461	dominant strains of both noroviruses and rotaviruses	446:497	FUT2 genetic polymorphisms impairing fucosylation are strongly associated with resistance to dominant strains of both noroviruses and rotaviruses.
36922975	8	72	theme	balanced	1774:1781	arg1	state					1797:1801	a balanced immunological state	1772:1801	a balanced immunological state that confers protection against inflammatory diseases	1772:1855	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	8	73	theme	T	1742:1742	arg1	lymphocytes					1744:1754	these T lymphocytes	1736:1754	these T lymphocytes	1736:1754	These observations link the FUT2 genotype with the risk of RVA gastroenteritis, the microbiota and microbiota-induced DP8α Treg cells, suggesting that the anti-RVA immune response might involve an induction/expansion of these T lymphocytes later providing a balanced immunological state that confers protection against inflammatory diseases.
36922975	5	74	theme	anti-RVA	1196:1203	arg1	antibodies					1218:1227	anti-RVA neutralizing antibodies	1196:1227	anti-RVA neutralizing antibodies	1196:1227	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	75	theme	regulatory	1111:1120	arg1	cells					1124:1128	gut microbiota-induced regulatory T cells	1088:1128	gut microbiota-induced regulatory T cells (Tregs)	1088:1136	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	75	theme	regulatory	1111:1120	arg1	Tregs					1154:1158	so-called DP8α Tregs	1139:1158	so-called DP8α Tregs	1139:1158	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	5	75	theme	regulatory	1111:1120	arg1	Tregs					1131:1135	Tregs	1131:1135	Tregs	1131:1135	Strikingly, it was also associated with the frequency of gut microbiota-induced regulatory T cells (Tregs), so-called DP8α Tregs, albeit only in individuals who had anti-RVA neutralizing antibodies or high titers of anti-RVA IgAs.
36922975	1	76	theme	glycans	189:195	arg1	synthesis					164:172	the synthesis	160:172	the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children	160:350	The FUT2 α1,2fucosyltransferase contributes to the synthesis of fucosylated glycans used as attachment factors by several pathogens, including noroviruses and rotaviruses, that can induce life-threatening gastroenteritis in young children.
35007571	4	0	theme	major	529:533	arg1	impact					544:549	a major negative impact	527:549	a major negative impact on coastal ecosystems and nearshore marine life	527:597	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	2	1	theme	brown	310:314	arg1	algae					316:320	these brown algae	304:320	these brown algae	304:320	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	1	2	theme	North	232:236	arg1	Ocean					247:251	the North Atlantic Ocean	228:251	the North Atlantic Ocean	228:251	Pelagic Sargassum species have been known for centuries in the Sargasso Sea of the North Atlantic Ocean.
35007571	9	3	theme	expensive	1549:1557	arg1	techniques					1575:1584	expensive (freeze) drying techniques	1549:1584	expensive (freeze) drying techniques	1549:1584	We also showed that cheap (sun) compared to expensive (freeze) drying techniques influence the biochemical composition of biomass.
35007571	6	4	theme	different	1185:1193	arg1	times					1195:1199	different times	1185:1199	different times	1185:1199	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	1	5	theme	Ocean	247:251	arg1	Sea					221:223	the Sargasso Sea	208:223	the Sargasso Sea of the North Atlantic Ocean	208:251	Pelagic Sargassum species have been known for centuries in the Sargasso Sea of the North Atlantic Ocean.
35007571	11	6	theme	sample	2023:2028	arg1	locations					2030:2038	the sample locations	2019:2038	the sample locations investigated	2019:2051	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	0	7	theme	Pelagic	0:6	arg1	events					18:23	Pelagic Sargassum events	0:23	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.	0:147	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	7	theme	Pelagic	0:6	arg1	Provenance					37:46	Provenance	37:46	Provenance	37:46	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	7	theme	Pelagic	0:6	arg1	abundance					60:68	morphotype abundance	49:68	morphotype abundance	49:68	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	7	theme	Pelagic	0:6	arg1	influence					75:83	influence	75:83	influence of sample processing on biochemical composition of the biomass	75:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	9	8	theme	drying	1568:1573	arg1	techniques					1575:1584	expensive (freeze) drying techniques	1549:1584	expensive (freeze) drying techniques	1549:1584	We also showed that cheap (sun) compared to expensive (freeze) drying techniques influence the biochemical composition of biomass.
35007571	11	9	theme	fatty	1985:1989	arg1	content					1996:2002	fatty acid content	1985:2002	fatty acid content	1985:2002	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	2	10	theme	area	269:272	arg1	biomass					293:299	a new area concentrating high biomass	263:299	a new area concentrating high biomass of these brown algae	263:320	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	5	11	theme	composition	815:825	arg1	forecasting					751:761	the forecasting	747:761	the forecasting of Sargassum events	747:781	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	11	theme	composition	815:825	arg1	elucidation					788:798	elucidation	788:798	elucidation of the seaweed composition	788:825	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	7	12	theme	mats	1305:1308	arg1	origin					1265:1270	the geographical origin	1248:1270	the geographical origin	1248:1270	No clear difference in the geographical origin, or provenance, of the Sargassum mats was observed.
35007571	7	12	theme	mats	1305:1308	arg1	provenance					1276:1285	provenance	1276:1285	provenance	1276:1285	No clear difference in the geographical origin, or provenance, of the Sargassum mats was observed.
35007571	4	13	theme	marine	587:592	arg1	life					594:597	nearshore marine life	577:597	nearshore marine life	577:597	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	5	14	theme	processing	992:1001	arg1	methods					1003:1009	sample processing methods	985:1009	sample processing methods	985:1009	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	10	15	theme	phenolic	1675:1682	arg1	compounds					1684:1692	phenolic compounds	1675:1692	phenolic compounds	1675:1692	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	9	16	theme	biochemical	1600:1610	arg1	composition					1612:1622	the biochemical composition	1596:1622	the biochemical composition of biomass	1596:1633	We also showed that cheap (sun) compared to expensive (freeze) drying techniques influence the biochemical composition of biomass.
35007571	10	17	theme	deleterious	1705:1715	arg1	impact					1717:1722	a deleterious impact	1703:1722	a deleterious impact on fucoxanthin content and on the quantities of monosaccharides	1703:1786	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	1	18	from	centuries	195:203	arg1	Sea					221:223	the Sargasso Sea	208:223	the Sargasso Sea of the North Atlantic Ocean	208:251	Pelagic Sargassum species have been known for centuries in the Sargasso Sea of the North Atlantic Ocean.
35007571	11	19	located	observed	1955:1962	arg2	variation					1941:1949	limited variation	1933:1949	limited variation	1933:1949	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	19	located	observed	1955:1962	arg1	locations					2030:2038	the sample locations	2019:2038	the sample locations investigated	2019:2051	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	19	located	observed	1955:1962	arg1	ash					1967:1969	ash	1967:1969	ash	1967:1969	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	19	located	observed	1955:1962	arg1	content					1996:2002	fatty acid content	1985:2002	fatty acid content	1985:2002	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	19	located	observed	1955:1962	arg1	most					2011:2014	most	2011:2014	most	2011:2014	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	19	located	observed	1955:1962	arg1	protein					1972:1978	protein	1972:1978	protein	1972:1978	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	20	theme	sulfated	1854:1861	arg1	polysaccharides					1863:1877	sulfated polysaccharides	1854:1877	sulfated polysaccharides	1854:1877	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	21	theme	acid	1991:1994	arg1	content					1996:2002	fatty acid content	1985:2002	fatty acid content	1985:2002	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	5	22	theme	morphotype	866:875	arg1	abundance					877:885	morphotype abundance	866:885	morphotype abundance	866:885	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	0	23	from	influence	75:83	arg1	composition					121:131	biochemical composition	109:131	biochemical composition of the biomass	109:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	24	theme	morphotype	49:58	arg1	events					18:23	Pelagic Sargassum events	0:23	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.	0:147	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	24	theme	morphotype	49:58	arg1	abundance					60:68	morphotype abundance	49:68	morphotype abundance	49:68	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	5	25	from	advances	735:742	arg1	forecasting					751:761	the forecasting	747:761	the forecasting of Sargassum events	747:781	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	25	from	advances	735:742	arg1	elucidation					788:798	elucidation	788:798	elucidation of the seaweed composition	788:825	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	26	theme	many	828:831	arg1	gaps					843:846	many knowledge gaps	828:846	many knowledge gaps	828:846	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	0	27	from	events	18:23	arg1	Jamaica					28:34	Jamaica	28:34	Jamaica	28:34	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	5	28	from	influence	972:980	arg1	months					941:946	the months	937:946	the months prior to stranding	937:965	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	28	from	influence	972:980	arg1	composition					1034:1044	biomass biochemical composition	1014:1044	biomass biochemical composition	1014:1044	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	1	29	theme	Pelagic	149:155	arg1	species					167:173	Pelagic Sargassum species	149:173	Pelagic Sargassum species	149:173	Pelagic Sargassum species have been known for centuries in the Sargasso Sea of the North Atlantic Ocean.
35007571	4	30	theme	maritime	700:707	arg1	transport					709:717	maritime transport	700:717	maritime transport	700:717	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	5	31	theme	biochemical	1022:1032	arg1	composition					1034:1044	biomass biochemical composition	1014:1044	biomass biochemical composition	1014:1044	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	0	32	from	Provenance	37:46	arg1	composition					121:131	biochemical composition	109:131	biochemical composition of the biomass	109:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	6	33	from	summer	1100:1105	arg1	coasts					1079:1084	the coasts	1075:1084	the coasts of Jamaica in summer of 2020	1075:1113	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	2	34	theme	Atlantic	357:364	arg1	Ocean					366:370	the Tropical Atlantic Ocean	344:370	the Tropical Atlantic Ocean	344:370	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	0	35	theme	sample	88:93	arg1	processing					95:104	sample processing	88:104	sample processing	88:104	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	10	36	from	impact	1717:1722	arg1	quantities					1758:1767	the quantities	1754:1767	the quantities of monosaccharides	1754:1786	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	10	36	from	impact	1717:1722	arg1	content					1739:1745	fucoxanthin content	1727:1745	fucoxanthin content	1727:1745	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	7	37	theme	geographical	1252:1263	arg1	origin					1265:1270	the geographical origin	1248:1270	the geographical origin	1248:1270	No clear difference in the geographical origin, or provenance, of the Sargassum mats was observed.
35007571	10	38	contain	had	1699:1701	arg2	impact					1717:1722	a deleterious impact	1703:1722	a deleterious impact on fucoxanthin content and on the quantities of monosaccharides	1703:1786	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	10	38	contain	had	1699:1701	arg1	Sun-drying					1636:1645	Sun-drying	1636:1645	Sun-drying	1636:1645	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	8	39	from	temperatures	1419:1430	arg1	range					1468:1472	the range	1464:1472	the range of 34-36 psu	1464:1485	The majority of Sargassum backtracked from both north and south of Jamaica experienced ambient temperatures of around 27 °C and salinity in the range of 34-36 psu before stranding.
35007571	6	40	from	coasts	1079:1084	arg1	summer					1100:1105	summer	1100:1105	summer of 2020	1100:1113	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	5	41	theme	Sargassum	766:774	arg1	events					776:781	Sargassum events	766:781	Sargassum events	766:781	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	3	42	dep	coast	474:478	arg1	off					466:468	off	466:468	off	466:468	Since then, massive and recurrent Sargassum influxes have been reported in the Caribbean and off the coast of Western Africa.
35007571	7	43	theme	clear	1228:1232	arg1	difference					1234:1243	No clear difference	1225:1243	No clear difference in the geographical origin, or provenance, of the Sargassum mats	1225:1308	No clear difference in the geographical origin, or provenance, of the Sargassum mats was observed.
35007571	6	44	from	Jamaica	1089:1095	arg1	summer					1100:1105	summer	1100:1105	summer of 2020	1100:1113	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	3	45	theme	Sargassum	407:415	arg1	influxes					417:424	massive and recurrent Sargassum influxes	385:424	massive and recurrent Sargassum influxes	385:424	Since then, massive and recurrent Sargassum influxes have been reported in the Caribbean and off the coast of Western Africa.
35007571	5	46	theme	recent	728:733	arg1	advances					735:742	recent advances	728:742	recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition	728:825	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	8	47	theme	34-36 psu	1477:1485	arg1	range					1468:1472	the range	1464:1472	the range of 34-36 psu	1464:1485	The majority of Sargassum backtracked from both north and south of Jamaica experienced ambient temperatures of around 27 °C and salinity in the range of 34-36 psu before stranding.
35007571	5	48	from	events	904:909	arg1	months					941:946	the months	937:946	the months prior to stranding	937:965	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	48	from	events	904:909	arg1	composition					1034:1044	biomass biochemical composition	1014:1044	biomass biochemical composition	1014:1044	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	6	49	from	times	1195:1199	arg1	morphotype					1171:1180	the most abundant morphotype	1153:1180	the most abundant morphotype	1153:1180	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	6	49	from	times	1195:1199	arg1	fluitans					1136:1143	fluitans	1136:1143	fluitans	1136:1143	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	0	50	dep	events	18:23	arg1	events					18:23	Pelagic Sargassum events	0:23	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.	0:147	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	50	dep	events	18:23	arg1	Provenance					37:46	Provenance	37:46	Provenance	37:46	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	50	dep	events	18:23	arg1	abundance					60:68	morphotype abundance	49:68	morphotype abundance	49:68	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	50	dep	events	18:23	arg1	influence					75:83	influence	75:83	influence of sample processing on biochemical composition of the biomass	75:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	7	51	from	difference	1234:1243	arg1	origin					1265:1270	the geographical origin	1248:1270	the geographical origin	1248:1270	No clear difference in the geographical origin, or provenance, of the Sargassum mats was observed.
35007571	7	51	from	difference	1234:1243	arg1	provenance					1276:1285	provenance	1276:1285	provenance	1276:1285	No clear difference in the geographical origin, or provenance, of the Sargassum mats was observed.
35007571	10	52	theme	monosaccharides	1772:1786	arg1	quantities					1758:1767	the quantities	1754:1767	the quantities of monosaccharides	1754:1786	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	8	53	theme	Sargassum	1340:1348	arg1	majority					1328:1335	The majority	1324:1335	The majority of Sargassum backtracked from both north and south of Jamaica	1324:1397	The majority of Sargassum backtracked from both north and south of Jamaica experienced ambient temperatures of around 27 °C and salinity in the range of 34-36 psu before stranding.
35007571	6	54	theme	sampling	1205:1212	arg1	locations					1214:1222	sampling locations	1205:1222	sampling locations	1205:1222	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	2	55	theme	algae	316:320	arg1	biomass					293:299	a new area concentrating high biomass	263:299	a new area concentrating high biomass of these brown algae	263:320	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	3	56	theme	Africa	491:496	arg1	coast					474:478	the coast	470:478	the coast of Western Africa	470:496	Since then, massive and recurrent Sargassum influxes have been reported in the Caribbean and off the coast of Western Africa.
35007571	5	57	from	drift	912:916	arg1	months					941:946	the months	937:946	the months prior to stranding	937:965	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	57	from	drift	912:916	arg1	composition					1034:1044	biomass biochemical composition	1014:1044	biomass biochemical composition	1014:1044	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	1	58	theme	Atlantic	238:245	arg1	Ocean					247:251	the North Atlantic Ocean	228:251	the North Atlantic Ocean	228:251	Pelagic Sargassum species have been known for centuries in the Sargasso Sea of the North Atlantic Ocean.
35007571	6	59	theme	2020	1110:1113	arg1	summer					1100:1105	summer	1100:1105	summer of 2020	1100:1113	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	4	60	theme	negative	535:542	arg1	impact					544:549	a major negative impact	527:549	a major negative impact on coastal ecosystems and nearshore marine life	527:597	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	1	61	theme	Sargassum	157:165	arg1	species					167:173	Pelagic Sargassum species	149:173	Pelagic Sargassum species	149:173	Pelagic Sargassum species have been known for centuries in the Sargasso Sea of the North Atlantic Ocean.
35007571	6	62	theme	abundant	1162:1169	arg1	morphotype					1171:1180	the most abundant morphotype	1153:1180	the most abundant morphotype	1153:1180	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	6	62	theme	abundant	1162:1169	arg1	fluitans					1136:1143	fluitans	1136:1143	fluitans	1136:1143	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	2	63	theme	concentrating	274:286	arg1	biomass					293:299	a new area concentrating high biomass	263:299	a new area concentrating high biomass of these brown algae	263:320	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	5	64	theme	seaweed	807:813	arg1	composition					815:825	the seaweed composition	803:825	the seaweed composition	803:825	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	6	65	theme	Jamaica	1089:1095	arg1	coasts					1079:1084	the coasts	1075:1084	the coasts of Jamaica in summer of 2020	1075:1113	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	4	66	theme	socio-economic	611:624	arg1	coastal					660:666	coastal	660:666	coastal	660:666	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	66	theme	socio-economic	611:624	arg1	transport					709:717	maritime transport	700:717	maritime transport	700:717	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	66	theme	socio-economic	611:624	arg1	tourism					676:682	tourism	676:682	tourism	676:682	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	66	theme	socio-economic	611:624	arg1	health					652:657	public health	645:657	public health	645:657	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	66	theme	socio-economic	611:624	arg1	fisheries					685:693	fisheries	685:693	fisheries	685:693	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	66	theme	socio-economic	611:624	arg1	sectors					626:632	socio-economic sectors	611:632	socio-economic sectors	611:632	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	0	67	theme	biochemical	109:119	arg1	composition					121:131	biochemical composition	109:131	biochemical composition of the biomass	109:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	2	68	theme	new	265:267	arg1	biomass					293:299	a new area concentrating high biomass	263:299	a new area concentrating high biomass of these brown algae	263:320	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	12	69	from	transport	2107:2115	arg1	context					2139:2145	the context	2135:2145	the context of its valorisation	2135:2165	These observations are important for the storage and transport of the biomass in the context of its valorisation.
35007571	5	70	theme	events	776:781	arg1	forecasting					751:761	the forecasting	747:761	the forecasting of Sargassum events	747:781	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	70	theme	events	776:781	arg1	elucidation					788:798	elucidation	788:798	elucidation of the seaweed composition	788:825	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	4	71	contain	have	522:525	arg2	impact					544:549	a major negative impact	527:549	a major negative impact on coastal ecosystems and nearshore marine life	527:597	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	71	contain	have	522:525	arg1	events					515:520	These Sargassum events	499:520	These Sargassum events	499:520	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	72	theme	nearshore	577:585	arg1	life					594:597	nearshore marine life	577:597	nearshore marine life	577:597	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	5	73	theme	methods	1003:1009	arg1	drift					912:916	drift	912:916	drift of the seaweeds in the months prior to stranding	912:965	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	73	theme	methods	1003:1009	arg1	events					904:909	Sargassum events	894:909	Sargassum events	894:909	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	73	theme	methods	1003:1009	arg1	influence					972:980	influence	972:980	influence of sample processing methods on biomass biochemical composition	972:1044	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	12	74	from	storage	2095:2101	arg1	context					2139:2145	the context	2135:2145	the context of its valorisation	2135:2165	These observations are important for the storage and transport of the biomass in the context of its valorisation.
35007571	11	75	contain	containing	1843:1852	arg2	polysaccharides					1863:1877	sulfated polysaccharides	1854:1877	sulfated polysaccharides	1854:1877	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	11	75	contain	containing	1843:1852	arg1	fucose					1836:1841	fucose	1836:1841	fucose containing sulfated polysaccharides	1836:1877	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	0	76	from	abundance	60:68	arg1	composition					121:131	biochemical composition	109:131	biochemical composition of the biomass	109:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	7	77	theme	Sargassum	1295:1303	arg1	mats					1305:1308	the Sargassum mats	1291:1308	the Sargassum mats	1291:1308	No clear difference in the geographical origin, or provenance, of the Sargassum mats was observed.
35007571	0	78	theme	biomass	140:146	arg1	composition					121:131	biochemical composition	109:131	biochemical composition of the biomass	109:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	5	79	theme	biomass	1014:1020	arg1	composition					1034:1044	biomass biochemical composition	1014:1044	biomass biochemical composition	1014:1044	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	9	80	theme	biomass	1627:1633	arg1	composition					1612:1622	the biochemical composition	1596:1622	the biochemical composition of biomass	1596:1633	We also showed that cheap (sun) compared to expensive (freeze) drying techniques influence the biochemical composition of biomass.
35007571	10	81	theme	compounds	1684:1692	arg1	proportion					1661:1670	the proportion	1657:1670	the proportion of phenolic compounds	1657:1692	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	8	82	theme	Jamaica	1391:1397	arg1	south					1382:1386	south	1382:1386	south	1382:1386	The majority of Sargassum backtracked from both north and south of Jamaica experienced ambient temperatures of around 27 °C and salinity in the range of 34-36 psu before stranding.
35007571	8	82	theme	Jamaica	1391:1397	arg1	north					1372:1376	north	1372:1376	north	1372:1376	The majority of Sargassum backtracked from both north and south of Jamaica experienced ambient temperatures of around 27 °C and salinity in the range of 34-36 psu before stranding.
35007571	12	83	theme	biomass	2124:2130	arg1	transport					2107:2115	transport	2107:2115	transport	2107:2115	These observations are important for the storage and transport of the biomass in the context of its valorisation.
35007571	12	83	theme	biomass	2124:2130	arg1	storage					2095:2101	storage	2095:2101	storage	2095:2101	These observations are important for the storage and transport of the biomass in the context of its valorisation.
35007571	5	84	theme	sample	985:990	arg1	methods					1003:1009	sample processing methods	985:1009	sample processing methods	985:1009	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	10	85	theme	fucoxanthin	1727:1737	arg1	content					1739:1745	fucoxanthin content	1727:1745	fucoxanthin content	1727:1745	Sun-drying increased the proportion of phenolic compounds, but had a deleterious impact on fucoxanthin content and on the quantities of monosaccharides, except for mannitol.
35007571	8	86	theme	ambient	1411:1417	arg1	temperatures					1419:1430	ambient temperatures	1411:1430	ambient temperatures of around 27 °C and salinity in the range of 34-36 psu	1411:1485	The majority of Sargassum backtracked from both north and south of Jamaica experienced ambient temperatures of around 27 °C and salinity in the range of 34-36 psu before stranding.
35007571	11	87	from	Effects	1810:1816	arg1	content					1825:1831	the content	1821:1831	the content of fucose containing sulfated polysaccharides	1821:1877	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	5	88	theme	knowledge	833:841	arg1	gaps					843:846	many knowledge gaps	828:846	many knowledge gaps	828:846	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	4	89	theme	public	645:650	arg1	health					652:657	public health	645:657	public health	645:657	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	90	from	impact	544:549	arg1	life					594:597	nearshore marine life	577:597	nearshore marine life	577:597	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	4	90	from	impact	544:549	arg1	ecosystems					562:571	coastal ecosystems	554:571	coastal ecosystems	554:571	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	11	91	theme	limited	1933:1939	arg1	variation					1941:1949	limited variation	1933:1949	limited variation	1933:1949	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	0	92	theme	processing	95:104	arg1	events					18:23	Pelagic Sargassum events	0:23	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.	0:147	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	92	theme	processing	95:104	arg1	Provenance					37:46	Provenance	37:46	Provenance	37:46	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	92	theme	processing	95:104	arg1	abundance					60:68	morphotype abundance	49:68	morphotype abundance	49:68	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	92	theme	processing	95:104	arg1	influence					75:83	influence	75:83	influence of sample processing on biochemical composition of the biomass	75:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	12	93	theme	valorisation	2154:2165	arg1	context					2139:2145	the context	2135:2145	the context of its valorisation	2135:2165	These observations are important for the storage and transport of the biomass in the context of its valorisation.
35007571	2	94	theme	Tropical	348:355	arg1	Ocean					366:370	the Tropical Atlantic Ocean	344:370	the Tropical Atlantic Ocean	344:370	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	0	95	theme	Sargassum	8:16	arg1	events					18:23	Pelagic Sargassum events	0:23	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.	0:147	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	95	theme	Sargassum	8:16	arg1	Provenance					37:46	Provenance	37:46	Provenance	37:46	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	95	theme	Sargassum	8:16	arg1	abundance					60:68	morphotype abundance	49:68	morphotype abundance	49:68	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	0	95	theme	Sargassum	8:16	arg1	influence					75:83	influence	75:83	influence of sample processing on biochemical composition of the biomass	75:146	Pelagic Sargassum events in Jamaica: Provenance, morphotype abundance, and influence of sample processing on biochemical composition of the biomass.
35007571	4	96	theme	Sargassum	505:513	arg1	events					515:520	These Sargassum events	499:520	These Sargassum events	499:520	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	3	97	theme	massive	385:391	arg1	influxes					417:424	massive and recurrent Sargassum influxes	385:424	massive and recurrent Sargassum influxes	385:424	Since then, massive and recurrent Sargassum influxes have been reported in the Caribbean and off the coast of Western Africa.
35007571	5	98	theme	Sargassum	894:902	arg1	events					904:909	Sargassum events	894:909	Sargassum events	894:909	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	11	99	theme	fucose	1836:1841	arg1	content					1825:1831	the content	1821:1831	the content of fucose containing sulfated polysaccharides	1821:1877	Effects on the content of fucose containing sulfated polysaccharides depended on the method used for their extraction, and limited variation was observed in ash, protein, and fatty acid content within most of the sample locations investigated.
35007571	5	100	theme	prior	948:952	arg1	months					941:946	the months	937:946	the months prior to stranding	937:965	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	6	101	from	locations	1214:1222	arg1	morphotype					1171:1180	the most abundant morphotype	1153:1180	the most abundant morphotype	1153:1180	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	6	101	from	locations	1214:1222	arg1	fluitans					1136:1143	fluitans	1136:1143	fluitans	1136:1143	Using seaweeds harvested on the coasts of Jamaica in summer of 2020, we observed that S. fluitans III was the most abundant morphotype at different times and sampling locations.
35007571	5	102	theme	seaweeds	925:932	arg1	drift					912:916	drift	912:916	drift of the seaweeds in the months prior to stranding	912:965	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	102	theme	seaweeds	925:932	arg1	events					904:909	Sargassum events	894:909	Sargassum events	894:909	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	5	102	theme	seaweeds	925:932	arg1	influence					972:980	influence	972:980	influence of sample processing methods on biomass biochemical composition	972:1044	Despite recent advances in the forecasting of Sargassum events, and elucidation of the seaweed composition, many knowledge gaps remain, including morphotype abundance during Sargassum events, drift of the seaweeds in the months prior to stranding, and influence of sample processing methods on biomass biochemical composition.
35007571	4	103	theme	coastal	554:560	arg1	ecosystems					562:571	coastal ecosystems	554:571	coastal ecosystems	554:571	These Sargassum events have a major negative impact on coastal ecosystems and nearshore marine life, and affect socio-economic sectors, including public health, coastal living, tourism, fisheries, and maritime transport.
35007571	2	104	theme	high	288:291	arg1	biomass					293:299	a new area concentrating high biomass	263:299	a new area concentrating high biomass of these brown algae	263:320	In 2011, a new area concentrating high biomass of these brown algae started developing in the Tropical Atlantic Ocean.
35007571	1	105	theme	Sargasso	212:219	arg1	Sea					221:223	the Sargasso Sea	208:223	the Sargasso Sea of the North Atlantic Ocean	208:251	Pelagic Sargassum species have been known for centuries in the Sargasso Sea of the North Atlantic Ocean.
35007571	3	106	theme	recurrent	397:405	arg1	influxes					417:424	massive and recurrent Sargassum influxes	385:424	massive and recurrent Sargassum influxes	385:424	Since then, massive and recurrent Sargassum influxes have been reported in the Caribbean and off the coast of Western Africa.
35007571	9	107	dep	expensive	1549:1557	arg1	freeze					1560:1565	freeze	1560:1565	freeze	1560:1565	We also showed that cheap (sun) compared to expensive (freeze) drying techniques influence the biochemical composition of biomass.
35401429	2	0	theme	liver	554:558	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	6	1	theme	subsequent	994:1003	arg1	analysis					1018:1025	A subsequent metabolomics analysis	992:1025	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based)	992:1065	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	3	2	theme	malonaldehyde	648:660	arg1	levels					630:635	decreased levels	620:635	decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content	620:700	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	3	2	theme	malonaldehyde	648:660	arg1	content					762:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	1	3	theme	polysaccharides	281:295	arg1	regulation					221:230	the regulation	217:230	the regulation of gut microbiota	217:248	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	3	theme	polysaccharides	281:295	arg1	effect					271:276	hepatoprotective effect	254:276	hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs)	254:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	2	4	theme	change	544:549	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	3	5	theme	carbonyl	680:687	arg1	content					694:700	protein carbonyl (PC) content	672:700	protein carbonyl (PC) content	672:700	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	6	6	theme	important	1183:1191	arg1	roles					1193:1197	important roles	1183:1197	important roles	1183:1197	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	1	7	theme	Flammulina	302:311	arg1	FVPs					324:327	FVPs	324:327	FVPs	324:327	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	7	theme	Flammulina	302:311	arg1	velutipes					313:321	Flammulina velutipes	302:321	Flammulina velutipes (FVPs)	302:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	6	8	theme	fatty	1081:1085	arg1	biosynthesis					1092:1103	fatty acid biosynthesis	1081:1103	fatty acid biosynthesis	1081:1103	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	7	9	theme	natural	1326:1332	arg1	products					1334:1341	natural products	1326:1341	natural products	1326:1341	This study provides a potential way to modulate gut microbiota and manage liver diseases using natural products.
35401429	3	10	theme	content	694:700	arg1	levels					630:635	decreased levels	620:635	decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content	620:700	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	3	10	theme	content	694:700	arg1	content					762:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	2	11	theme	hepatoprotective	335:350	arg1	effect					352:357	The hepatoprotective effect	331:357	The hepatoprotective effect of the FVPs	331:369	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	4	12	theme	pro-inflammatory	843:858	arg1	TNF-α					898:902	TNF-α	898:902	TNF-α	898:902	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	4	12	theme	pro-inflammatory	843:858	arg1	IL-1β					887:891	IL-1β	887:891	IL-1β	887:891	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	4	12	theme	pro-inflammatory	843:858	arg1	cytokines					860:868	pro-inflammatory cytokines	843:868	pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α)	843:903	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	4	12	theme	pro-inflammatory	843:858	arg1	IL-6					881:884	IL-6	881:884	IL-6	881:884	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	2	13	from	levels	388:393	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	3	14	theme	PC	690:691	arg1	content					694:700	protein carbonyl (PC) content	672:700	protein carbonyl (PC) content	672:700	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	2	15	theme	bile	515:518	arg1	content					531:537	total bile acid (TBA) content	509:537	total bile acid (TBA) content	509:537	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	6	16	theme	xenobiotics	1147:1157	arg1	metabolism					1117:1126	tryptophan metabolism	1106:1126	tryptophan metabolism	1106:1126	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	6	16	theme	xenobiotics	1147:1157	arg1	metabolism					1133:1142	metabolism	1133:1142	metabolism	1133:1142	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	6	16	theme	xenobiotics	1147:1157	arg1	biosynthesis					1092:1103	fatty acid biosynthesis	1081:1103	fatty acid biosynthesis	1081:1103	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	5	17	theme	microbiota	980:989	arg1	composition					957:967	the composition	953:967	the composition of the gut microbiota	953:989	16S rRNA sequencing shows that the FVPs change the composition of the gut microbiota.
35401429	1	18	theme	gut	235:237	arg1	microbiota					239:248	gut microbiota	235:248	gut microbiota	235:248	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	3	19	theme	increased	706:714	arg1	content					762:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	1	20	from	effect	271:276	arg1	FVPs					324:327	FVPs	324:327	FVPs	324:327	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	20	from	effect	271:276	arg1	velutipes					313:321	Flammulina velutipes	302:321	Flammulina velutipes (FVPs)	302:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	4	21	from	decrease	831:838	arg1	TNF-α					898:902	TNF-α	898:902	TNF-α	898:902	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	4	21	from	decrease	831:838	arg1	IL-1β					887:891	IL-1β	887:891	IL-1β	887:891	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	4	21	from	decrease	831:838	arg1	cytokines					860:868	pro-inflammatory cytokines	843:868	pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α)	843:903	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	4	21	from	decrease	831:838	arg1	IL-6					881:884	IL-6	881:884	IL-6	881:884	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	1	22	theme	carbon	140:145	arg1	injury					181:186	carbon tetrachloride-induced acute liver injury	140:186	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	3	23	theme	superoxide	735:744	arg1	SOD					757:759	SOD	757:759	SOD	757:759	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	3	23	theme	superoxide	735:744	arg1	dismutase					746:754	superoxide dismutase	735:754	superoxide dismutase (SOD)	735:760	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	6	24	theme	hepatoprotective	1206:1221	arg1	effect					1223:1228	the hepatoprotective effect	1202:1228	the hepatoprotective effect	1202:1228	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	3	25	theme	anti-oxidant	582:593	arg1	activity					595:602	Their anti-oxidant activity	576:602	Their anti-oxidant activity	576:602	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	1	26	theme	acute	169:173	arg1	injury					181:186	carbon tetrachloride-induced acute liver injury	140:186	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	2	27	theme	cholesterol	491:501	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	28	from	Injury	122:127	arg1	Mice					132:135	Mice	132:135	Mice	132:135	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	6	29	theme	tryptophan	1106:1115	arg1	metabolism					1117:1126	tryptophan metabolism	1106:1126	tryptophan metabolism	1106:1126	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	0	30	theme	Carbon	75:80	arg1	Injury					122:127	Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury	46:127	Injury	122:127	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	1	31	theme	injury	181:186	arg1	model					194:198	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	2	32	from	aminotransferase	442:457	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	33	theme	Hepatic	104:110	arg1	Injury					122:127	Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury	46:127	Injury	122:127	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	7	34	theme	potential	1253:1261	arg1	way					1263:1265	a potential way	1251:1265	a potential way to modulate gut microbiota and manage liver diseases using natural products	1251:1341	This study provides a potential way to modulate gut microbiota and manage liver diseases using natural products.
35401429	2	35	theme	aspartate	404:412	arg1	transaminase					414:425	serum aspartate transaminase	398:425	serum aspartate transaminase (AST)	398:431	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	35	theme	aspartate	404:412	arg1	AST					428:430	AST	428:430	AST	428:430	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	36	theme	Flammulina	0:9	arg1	Polysaccharides					21:35	Flammulina velutipes Polysaccharides	0:35	Flammulina velutipes Polysaccharides	0:35	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	1	37	used	used	203:206	arg2	model					194:198	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	2	38	theme	alanine	434:440	arg1	ALT					460:462	ALT	460:462	ALT	460:462	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	38	theme	alanine	434:440	arg1	aminotransferase					442:457	alanine aminotransferase	434:457	alanine aminotransferase (ALT)	434:463	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	39	theme	Gut	46:48	arg1	Microbiota					50:59	Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury	46:127	Microbiota	50:59	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	2	40	theme	triglyceride	466:477	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	41	from	histopathology	560:573	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	6	42	theme	gut	1034:1036	arg1	bacteria					1038:1045	the gut bacteria	1030:1045	the gut bacteria (UHPLC-MS/MS-based)	1030:1065	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	3	43	theme	hepatic	640:646	arg1	MDA					663:665	MDA	663:665	MDA	663:665	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	3	43	theme	hepatic	640:646	arg1	malonaldehyde					648:660	hepatic malonaldehyde	640:660	hepatic malonaldehyde (MDA)	640:666	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	1	44	theme	microbiota	239:248	arg1	regulation					221:230	the regulation	217:230	the regulation of gut microbiota	217:248	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	44	theme	microbiota	239:248	arg1	effect					271:276	hepatoprotective effect	254:276	hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs)	254:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	5	45	theme	16S	906:908	arg1	sequencing					915:924	16S rRNA sequencing	906:924	16S rRNA sequencing	906:924	16S rRNA sequencing shows that the FVPs change the composition of the gut microbiota.
35401429	6	46	dep	bacteria	1038:1045	arg1	UHPLC-MS/MS-based					1048:1064	UHPLC-MS/MS-based	1048:1064	UHPLC-MS/MS-based	1048:1064	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	1	47	theme	hepatoprotective	254:269	arg1	effect					271:276	hepatoprotective effect	254:276	hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs)	254:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	6	48	theme	metabolomics	1005:1016	arg1	analysis					1018:1025	A subsequent metabolomics analysis	992:1025	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based)	992:1065	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	2	49	theme	reduced	380:386	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	1	50	from	velutipes	313:321	arg1	regulation					221:230	the regulation	217:230	the regulation of gut microbiota	217:248	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	50	from	velutipes	313:321	arg1	effect					271:276	hepatoprotective effect	254:276	hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs)	254:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	50	from	velutipes	313:321	arg1	polysaccharides					281:295	polysaccharides	281:295	polysaccharides from Flammulina velutipes (FVPs)	281:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	4	51	theme	FVPs	808:811	arg1	ability					793:799	The anti-inflammatory ability	771:799	The anti-inflammatory ability of the FVPs	771:811	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	6	52	theme	acid	1087:1090	arg1	biosynthesis					1092:1103	fatty acid biosynthesis	1081:1103	fatty acid biosynthesis	1081:1103	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	2	53	from	content	531:537	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	3	54	theme	protein	672:678	arg1	content					694:700	protein carbonyl (PC) content	672:700	protein carbonyl (PC) content	672:700	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	6	55	theme	cytochrome	1162:1171	arg1	P450					1173:1176	cytochrome P450	1162:1176	cytochrome P450	1162:1176	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	4	56	theme	anti-inflammatory	775:791	arg1	ability					793:799	The anti-inflammatory ability	771:799	The anti-inflammatory ability of the FVPs	771:811	The anti-inflammatory ability of the FVPs is reflected in a decrease in pro-inflammatory cytokines (including IL-6, IL-1β, and TNF-α).
35401429	2	57	theme	acid	520:523	arg1	content					531:537	total bile acid (TBA) content	509:537	total bile acid (TBA) content	509:537	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	5	58	theme	gut	976:978	arg1	microbiota					980:989	the gut microbiota	972:989	the gut microbiota	972:989	16S rRNA sequencing shows that the FVPs change the composition of the gut microbiota.
35401429	2	59	theme	total	509:513	arg1	content					531:537	total bile acid (TBA) content	509:537	total bile acid (TBA) content	509:537	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	60	from	cholesterol	491:501	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	7	61	theme	liver	1305:1309	arg1	diseases					1311:1318	liver diseases	1305:1318	liver diseases using natural products	1305:1341	This study provides a potential way to modulate gut microbiota and manage liver diseases using natural products.
35401429	2	62	theme	content	531:537	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	3	63	theme	catalase	716:723	arg1	content					762:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	1	64	from	regulation	221:230	arg1	FVPs					324:327	FVPs	324:327	FVPs	324:327	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	64	from	regulation	221:230	arg1	velutipes					313:321	Flammulina velutipes	302:321	Flammulina velutipes (FVPs)	302:328	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	1	65	theme	tetrachloride-induced	147:167	arg1	injury					181:186	carbon tetrachloride-induced acute liver injury	140:186	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	2	66	theme	TBA	526:528	arg1	content					531:537	total bile acid (TBA) content	509:537	total bile acid (TBA) content	509:537	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	7	67	theme	gut	1279:1281	arg1	microbiota					1283:1292	gut microbiota	1279:1292	gut microbiota	1279:1292	This study provides a potential way to modulate gut microbiota and manage liver diseases using natural products.
35401429	3	68	theme	dismutase	746:754	arg1	content					762:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	increased catalase (CAT) and superoxide dismutase (SOD) content	706:768	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	0	69	theme	Tetrachloride-Induced	82:102	arg1	Injury					122:127	Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury	46:127	Injury	122:127	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	1	70	theme	liver	175:179	arg1	injury					181:186	carbon tetrachloride-induced acute liver injury	140:186	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	2	71	theme	total	485:489	arg1	TC					504:505	TC	504:505	TC	504:505	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	71	theme	total	485:489	arg1	cholesterol					491:501	total cholesterol	485:501	total cholesterol (TC)	485:506	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	72	theme	Oxidative	112:120	arg1	Injury					122:127	Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury	46:127	Injury	122:127	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	1	73	theme	mouse	188:192	arg1	model					194:198	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model	138:198	A carbon tetrachloride-induced acute liver injury mouse model is used to study the regulation of gut microbiota and hepatoprotective effect of polysaccharides from Flammulina velutipes (FVPs).
35401429	2	74	from	triglyceride	466:477	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	75	from	Microbiota	50:59	arg1	Mice					132:135	Mice	132:135	Mice	132:135	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	2	76	theme	transaminase	414:425	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	77	theme	velutipes	11:19	arg1	Polysaccharides					21:35	Flammulina velutipes Polysaccharides	0:35	Flammulina velutipes Polysaccharides	0:35	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	2	78	from	change	544:549	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	79	theme	serum	398:402	arg1	transaminase					414:425	serum aspartate transaminase	398:425	serum aspartate transaminase (AST)	398:431	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	79	theme	serum	398:402	arg1	AST					428:430	AST	428:430	AST	428:430	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	80	theme	aminotransferase	442:457	arg1	levels					388:393	reduced levels	380:393	reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology	380:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	0	81	theme	Alleviate	65:73	arg1	Injury					122:127	Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury	46:127	Injury	122:127	Flammulina velutipes Polysaccharides Modulate Gut Microbiota and Alleviate Carbon Tetrachloride-Induced Hepatic Oxidative Injury in Mice.
35401429	6	82	theme	bacteria	1038:1045	arg1	analysis					1018:1025	A subsequent metabolomics analysis	992:1025	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based)	992:1065	A subsequent metabolomics analysis of the gut bacteria (UHPLC-MS/MS-based) revealed that fatty acid biosynthesis, tryptophan metabolism, and metabolism of xenobiotics by cytochrome P450 play important roles in the hepatoprotective effect.
35401429	2	83	theme	FVPs	366:369	arg1	effect					352:357	The hepatoprotective effect	331:357	The hepatoprotective effect of the FVPs	331:369	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	2	84	from	transaminase	414:425	arg1	histopathology					560:573	liver histopathology	554:573	liver histopathology	554:573	The hepatoprotective effect of the FVPs leads to reduced levels of serum aspartate transaminase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), total bile acid (TBA) content, and change in liver histopathology.
35401429	3	85	theme	decreased	620:628	arg1	levels					630:635	decreased levels	620:635	decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content	620:700	Their anti-oxidant activity is exhibited by decreased levels of hepatic malonaldehyde (MDA) and protein carbonyl (PC) content and increased catalase (CAT) and superoxide dismutase (SOD) content.
35401429	5	86	theme	rRNA	910:913	arg1	sequencing					915:924	16S rRNA sequencing	906:924	16S rRNA sequencing	906:924	16S rRNA sequencing shows that the FVPs change the composition of the gut microbiota.
35268116	4	0	theme	tract	796:800	arg1	digestibility					809:821	greater ileal and total tract starch digestibility	772:821	greater ileal and total tract starch digestibility	772:821	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	4	1	theme	insoluble	862:870	arg1	degradability					876:888	insoluble NSP degradability	862:888	insoluble NSP degradability	862:888	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	3	2	theme	medium	689:694	arg1	p					708:708	p	708:708	p &lt; 0.005	708:719	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	3	2	theme	medium	689:694	arg1	level					701:705	the medium sNSP level	685:705	the medium sNSP level (p &lt; 0.005)	685:720	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	4	3	theme	total	790:794	arg1	digestibility					809:821	greater ileal and total tract starch digestibility	772:821	greater ileal and total tract starch digestibility	772:821	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	1	4	dep	d	304:304	arg1	to					308:309	to	308:309	to	308:309	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	3	5	theme	sNSP	696:699	arg1	p					708:708	p	708:708	p &lt; 0.005	708:719	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	3	5	theme	sNSP	696:699	arg1	level					701:705	the medium sNSP level	685:705	the medium sNSP level (p &lt; 0.005)	685:720	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	0	6	theme	Non-Starch	114:123	arg1	Polysaccharides					125:139	Non-Starch Polysaccharides	114:139	Non-Starch Polysaccharides in Broiler Chickens	114:159	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	3	7	theme	low	583:585	arg1	level					592:596	the low sNSP level	579:596	the low sNSP level	579:596	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	1	8	from	effect	187:192	arg1	performance					250:260	performance	250:260	performance	250:260	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	1	8	from	effect	187:192	arg1	utilisation					275:285	nutrient utilisation	266:285	nutrient utilisation	266:285	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	4	9	theme	sNSP	751:754	arg1	level					756:760	the high sNSP level	742:760	the high sNSP level	742:760	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	3	10	from	d	655:655	arg1	intake					645:650	lower feed intake	634:650	lower feed intake at d 14-35	634:661	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	3	10	from	d	655:655	arg1	BWG					616:618	greater BWG	608:618	greater BWG at d 0-14	608:628	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	7	11	theme	bird	1314:1317	arg1	performance					1319:1329	bird performance	1314:1329	bird performance	1314:1329	These results illustrate that dietary sNSP level and composition influences bird performance and nutrient digestibility.
35268116	2	12	theme	sNSP	475:478	arg1	content					480:486	low sNSP content	471:486	low sNSP content	471:486	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	0	13	from	Performance	59:69	arg1	Chickens					152:159	Broiler Chickens	144:159	Broiler Chickens	144:159	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	3	14	from	d	623:623	arg1	intake					645:650	lower feed intake	634:650	lower feed intake at d 14-35	634:661	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	3	14	from	d	623:623	arg1	BWG					616:618	greater BWG	608:618	greater BWG at d 0-14	608:628	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	0	15	theme	Polysaccharides	125:139	arg1	Disappearance					97:109	Disappearance	97:109	Disappearance	97:109	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	0	15	theme	Polysaccharides	125:139	arg1	Performance					59:69	Performance	59:69	Performance	59:69	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	0	15	theme	Polysaccharides	125:139	arg1	Utilisation					81:91	Nutrient Utilisation	72:91	Nutrient Utilisation	72:91	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	4	16	theme	tract	833:837	arg1	degradability					844:856	total tract sNSP degradability	827:856	total tract sNSP degradability	827:856	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	1	17	theme	dietary	197:203	arg1	sNSP					241:244	sNSP	241:244	sNSP	241:244	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	1	17	theme	dietary	197:203	arg1	polysaccharides					224:238	dietary soluble non-starch polysaccharides	197:238	dietary soluble non-starch polysaccharides (sNSP)	197:245	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	0	18	theme	Soluble	8:14	arg1	Level					42:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	3	19	theme	greater	608:614	arg1	BWG					616:618	greater BWG	608:618	greater BWG at d 0-14	608:628	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	1	20	from	d	304:304	arg1	broilers					290:297	broilers	290:297	broilers from d 0 to 35	290:312	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	1	21	theme	soluble	205:211	arg1	sNSP					241:244	sNSP	241:244	sNSP	241:244	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	1	21	theme	soluble	205:211	arg1	polysaccharides					224:238	dietary soluble non-starch polysaccharides	197:238	dietary soluble non-starch polysaccharides (sNSP)	197:245	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	0	22	theme	Dietary	0:6	arg1	Level					42:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	4	23	theme	sNSP	919:922	arg1	level					924:928	the low sNSP level	911:928	the low sNSP level	911:928	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	1	24	theme	non-starch	213:222	arg1	sNSP					241:244	sNSP	241:244	sNSP	241:244	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	1	24	theme	non-starch	213:222	arg1	polysaccharides					224:238	dietary soluble non-starch polysaccharides	197:238	dietary soluble non-starch polysaccharides (sNSP)	197:245	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	0	25	theme	Polysaccharide	27:40	arg1	Level					42:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	3	26	theme	lower	634:638	arg1	intake					645:650	lower feed intake	634:650	lower feed intake at d 14-35	634:661	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	2	27	theme	×	496:496	arg1	arrangement					510:520	a 2 × 3 factorial arrangement	492:520	a 2 × 3 factorial arrangement	492:520	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	2	28	theme	Cobb	315:318	arg1	broilers					324:331	Cobb 500 broilers	315:331	Cobb 500 broilers (n = 480, 80 birds per treatment)	315:365	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	4	29	theme	low	915:917	arg1	level					924:928	the low sNSP level	911:928	the low sNSP level	911:928	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	1	30	theme	polysaccharides	224:238	arg1	effect					187:192	the effect	183:192	the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation	183:285	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35268116	0	31	theme	Non-Starch	16:25	arg1	Level					42:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level	0:46	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	5	32	theme	gross	979:983	arg1	energy					985:990	gross energy	979:990	gross energy	979:990	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	0	33	theme	Broiler	144:150	arg1	Chickens					152:159	Broiler Chickens	144:159	Broiler Chickens	144:159	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	0	34	from	Disappearance	97:109	arg1	Chickens					152:159	Broiler Chickens	144:159	Broiler Chickens	144:159	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	5	35	theme	low	1073:1075	arg1	level					1082:1086	the high or low sNSP level	1061:1086	the high or low sNSP level (p &lt; 0.005)	1061:1101	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	5	35	theme	low	1073:1075	arg1	p					1089:1089	p	1089:1089	p &lt; 0.005	1089:1100	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	2	36	theme	high	454:457	arg1	medium					460:465	medium	460:465	medium	460:465	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	5	37	theme	energy	985:990	arg1	DM					952:953	total tract DM	940:953	total tract DM	940:953	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	5	37	theme	energy	985:990	arg1	metabolisability					959:974	metabolisability	959:974	metabolisability	959:974	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	4	38	theme	high	746:749	arg1	level					756:760	the high sNSP level	742:760	the high sNSP level	742:760	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	6	39	theme	corn-based	1186:1195	arg1	diets					1197:1201	the corn-based diets	1182:1201	the corn-based diets compared to the wheat-based diets	1182:1235	Generally, bird performance and nutrient utilisation was greater in birds fed the corn-based diets compared to the wheat-based diets.
35268116	2	40	theme	wheat-	383:388	arg1	diets					417:421	either wheat- or corn-soybean meal-based diets	376:421	either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35)	376:566	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	4	41	theme	starch	802:807	arg1	digestibility					809:821	greater ileal and total tract starch digestibility	772:821	greater ileal and total tract starch digestibility	772:821	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	5	42	theme	total	940:944	arg1	DM					952:953	total tract DM	940:953	total tract DM	940:953	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	0	43	from	Utilisation	81:91	arg1	Chickens					152:159	Broiler Chickens	144:159	Broiler Chickens	144:159	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	5	44	theme	medium	1021:1026	arg1	level					1033:1037	the medium sNSP level	1017:1037	the medium sNSP level	1017:1037	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	2	45	theme	low	471:473	arg1	content					480:486	low sNSP content	471:486	low sNSP content	471:486	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	4	46	theme	total	827:831	arg1	degradability					844:856	total tract sNSP degradability	827:856	total tract sNSP degradability	827:856	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	5	47	theme	tract	946:950	arg1	DM					952:953	total tract DM	940:953	total tract DM	940:953	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	2	48	dep	medium	460:465	arg1	a					452:452	a	452:452	a	452:452	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	4	49	theme	ileal	780:784	arg1	digestibility					809:821	greater ileal and total tract starch digestibility	772:821	greater ileal and total tract starch digestibility	772:821	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	5	50	theme	sNSP	1028:1031	arg1	level					1033:1037	the medium sNSP level	1017:1037	the medium sNSP level	1017:1037	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	3	51	theme	feed	640:643	arg1	intake					645:650	lower feed intake	634:650	lower feed intake at d 14-35	634:661	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	7	52	theme	dietary	1268:1274	arg1	level					1281:1285	dietary sNSP level	1268:1285	dietary sNSP level	1268:1285	These results illustrate that dietary sNSP level and composition influences bird performance and nutrient digestibility.
35268116	7	53	theme	sNSP	1276:1279	arg1	level					1281:1285	dietary sNSP level	1268:1285	dietary sNSP level	1268:1285	These results illustrate that dietary sNSP level and composition influences bird performance and nutrient digestibility.
35268116	2	54	theme	factorial	500:508	arg1	arrangement					510:520	a 2 × 3 factorial arrangement	492:520	a 2 × 3 factorial arrangement	492:520	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	0	55	from	Polysaccharides	125:139	arg1	Chickens					152:159	Broiler Chickens	144:159	Broiler Chickens	144:159	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	2	56	dep	broilers	324:331	arg1	=					336:336	=	336:336	=	336:336	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	4	57	dep	ileal	780:784	arg1	greater					772:778	greater	772:778	greater	772:778	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	5	58	theme	sNSP	1077:1080	arg1	level					1082:1086	the high or low sNSP level	1061:1086	the high or low sNSP level (p &lt; 0.005)	1061:1101	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	5	58	theme	sNSP	1077:1080	arg1	p					1089:1089	p	1089:1089	p &lt; 0.005	1089:1100	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	5	59	theme	high	1065:1068	arg1	level					1082:1086	the high or low sNSP level	1061:1086	the high or low sNSP level (p &lt; 0.005)	1061:1101	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	5	59	theme	high	1065:1068	arg1	p					1089:1089	p	1089:1089	p &lt; 0.005	1089:1100	At d 35, total tract DM and metabolisability of gross energy was greater in birds fed the medium sNSP level compared to those fed the high or low sNSP level (p &lt; 0.005).
35268116	7	60	theme	nutrient	1335:1342	arg1	digestibility					1344:1356	nutrient digestibility	1335:1356	nutrient digestibility	1335:1356	These results illustrate that dietary sNSP level and composition influences bird performance and nutrient digestibility.
35268116	6	61	theme	nutrient	1136:1143	arg1	utilisation					1145:1155	nutrient utilisation	1136:1155	nutrient utilisation	1136:1155	Generally, bird performance and nutrient utilisation was greater in birds fed the corn-based diets compared to the wheat-based diets.
35268116	6	62	theme	bird	1115:1118	arg1	performance					1120:1130	bird performance	1115:1130	bird performance	1115:1130	Generally, bird performance and nutrient utilisation was greater in birds fed the corn-based diets compared to the wheat-based diets.
35268116	2	63	theme	corn-soybean	393:404	arg1	diets					417:421	either wheat- or corn-soybean meal-based diets	376:421	either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35)	376:566	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	6	64	theme	wheat-based	1219:1229	arg1	diets					1231:1235	the wheat-based diets	1215:1235	the wheat-based diets	1215:1235	Generally, bird performance and nutrient utilisation was greater in birds fed the corn-based diets compared to the wheat-based diets.
35268116	0	65	from	Chickens	152:159	arg1	Disappearance					97:109	Disappearance	97:109	Disappearance	97:109	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	0	65	from	Chickens	152:159	arg1	Performance					59:69	Performance	59:69	Performance	59:69	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	0	65	from	Chickens	152:159	arg1	Utilisation					81:91	Nutrient Utilisation	72:91	Nutrient Utilisation	72:91	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	0	66	theme	Nutrient	72:79	arg1	Utilisation					81:91	Nutrient Utilisation	72:91	Nutrient Utilisation	72:91	Dietary Soluble Non-Starch Polysaccharide Level Influences Performance, Nutrient Utilisation and Disappearance of Non-Starch Polysaccharides in Broiler Chickens.
35268116	3	67	theme	sNSP	587:590	arg1	level					592:596	the low sNSP level	579:596	the low sNSP level	579:596	Birds fed the low sNSP level presented greater BWG at d 0-14 and lower feed intake at d 14-35 compared to birds fed the medium sNSP level (p &lt; 0.005).
35268116	2	68	theme	meal-based	406:415	arg1	diets					417:421	either wheat- or corn-soybean meal-based diets	376:421	either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35)	376:566	Cobb 500 broilers (n = 480, 80 birds per treatment) were fed either wheat- or corn-soybean meal-based diets formulated to contain either a high, medium, or low sNSP content, in a 2 × 3 factorial arrangement, fed as Starter (d 0-14) and Grower (d 14-35).
35268116	4	69	attach	presented	762:770	arg2	birds					732:736	birds	732:736	birds fed the high sNSP level	732:760	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	4	69	attach	presented	762:770	arg1	d					726:726	d 14	726:729	d 14	726:729	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	4	70	theme	sNSP	839:842	arg1	degradability					844:856	total tract sNSP degradability	827:856	total tract sNSP degradability	827:856	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	4	71	theme	NSP	872:874	arg1	degradability					876:888	insoluble NSP degradability	862:888	insoluble NSP degradability	862:888	At d 14, birds fed the high sNSP level presented greater ileal and total tract starch digestibility and total tract sNSP degradability and insoluble NSP degradability, compared to feeding the low sNSP level.
35268116	1	72	theme	nutrient	266:273	arg1	utilisation					275:285	nutrient utilisation	266:285	nutrient utilisation	266:285	This study evaluated the effect of dietary soluble non-starch polysaccharides (sNSP) on performance and nutrient utilisation in broilers from d 0 to 35.
35159542	0	0	theme	Activities	84:93	arg1	Metabolism					43:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	0	0	theme	Activities	84:93	arg1	Changes					58:64	Changes	58:64	Changes	58:64	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	4	1	theme	cell	690:693	arg1	enzymes					710:716	cell wall-degrading enzymes	690:716	cell wall-degrading enzymes	690:716	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	2	theme	enzymes	710:716	arg1	polygalacturonase					727:743	polygalacturonase	727:743	polygalacturonase	727:743	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	2	theme	enzymes	710:716	arg1	cellulase					746:754	cellulase	746:754	cellulase	746:754	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	2	theme	enzymes	710:716	arg1	β-galactosidase					761:775	β-galactosidase	761:775	β-galactosidase	761:775	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	2	theme	enzymes	710:716	arg1	activities					676:685	lower activities	670:685	lower activities	670:685	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	1	3	theme	Zizania	164:170	arg1	wall					187:190	the Zizania latifolia cell wall	160:190	the Zizania latifolia cell wall	160:190	The metabolism of polysaccharides in the Zizania latifolia cell wall helps maintain the postharvest quality during storage.
35159542	0	4	theme	Enzyme	77:82	arg1	Activities					84:93	Related Enzyme Activities	69:93	Related Enzyme Activities	69:93	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	2	5	theme	polysaccharide	331:344	arg1	composition					346:356	cell wall polysaccharide composition	321:356	cell wall polysaccharide composition	321:356	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	0	6	theme	Postharvest	102:112	arg1	Storage					114:120	Postharvest Storage	102:120	Postharvest Storage	102:120	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	4	7	theme	esterification	1040:1053	arg1	degree					1030:1035	the degree	1026:1035	the degree of esterification of water-soluble polysaccharides at 25 °C	1026:1095	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	3	8	from	increase	516:523	arg1	content					549:555	water-soluble pectin content	528:555	water-soluble pectin content during storage	528:570	The results showed that hardness declined concomitantly with an increase in water-soluble pectin content during storage, as well as with a decrease in propectin and cellulose contents.
35159542	2	9	theme	composition	346:356	arg1	activities					440:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	1	10	theme	polysaccharides	141:155	arg1	metabolism					127:136	The metabolism	123:136	The metabolism of polysaccharides in the Zizania latifolia cell wall	123:190	The metabolism of polysaccharides in the Zizania latifolia cell wall helps maintain the postharvest quality during storage.
35159542	4	11	theme	spectroscopy	948:959	arg1	results					928:934	the results	924:934	the results of infrared spectroscopy	924:959	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	12	from	β-galactosidase	761:775	arg1	Z.					780:781	Z.	780:781	Z.	780:781	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	3	13	theme	water-soluble	528:540	arg1	content					549:555	water-soluble pectin content	528:555	water-soluble pectin content during storage	528:570	The results showed that hardness declined concomitantly with an increase in water-soluble pectin content during storage, as well as with a decrease in propectin and cellulose contents.
35159542	4	14	dep	those	887:891	arg1	stored					893:898	stored	893:898	stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C	893:1120	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	1	15	theme	cell	182:185	arg1	wall					187:190	the Zizania latifolia cell wall	160:190	the Zizania latifolia cell wall	160:190	The metabolism of polysaccharides in the Zizania latifolia cell wall helps maintain the postharvest quality during storage.
35159542	4	16	theme	infrared	939:946	arg1	spectroscopy					948:959	infrared spectroscopy	939:959	infrared spectroscopy	939:959	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	17	from	degree	1030:1035	arg1	°C					1094:1095	25 °C	1091:1095	25 °C	1091:1095	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	18	theme	lighter	830:836	arg1	fiberization					838:849	lighter fiberization	830:849	lighter fiberization	830:849	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	0	19	theme	Zizania	0:6	arg1	Metabolism					43:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	0	20	theme	latifolia	8:16	arg1	Metabolism					43:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	4	21	theme	texture	973:979	arg1	softening					981:989	texture softening	973:989	texture softening	973:989	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	22	theme	greater	855:861	arg1	hardness					863:870	greater hardness	855:870	greater hardness	855:870	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	1	23	dep	Zizania	164:170	arg1	latifolia					172:180	latifolia	172:180	latifolia	172:180	The metabolism of polysaccharides in the Zizania latifolia cell wall helps maintain the postharvest quality during storage.
35159542	2	24	theme	structure	369:377	arg1	activities					440:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	2	25	theme	enzyme	433:438	arg1	activities					440:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	4	26	from	cellulase	746:754	arg1	Z.					780:781	Z.	780:781	Z.	780:781	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	2	27	theme	wall	364:367	arg1	structure					369:377	cell wall structure	359:377	cell wall structure	359:377	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	0	28	theme	Wall	23:26	arg1	Metabolism					43:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	1	29	from	metabolism	127:136	arg1	wall					187:190	the Zizania latifolia cell wall	160:190	the Zizania latifolia cell wall	160:190	The metabolism of polysaccharides in the Zizania latifolia cell wall helps maintain the postharvest quality during storage.
35159542	2	30	theme	cell	404:407	arg1	wall					409:412	cell wall	404:412	cell wall	404:412	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	2	31	theme	wall	409:412	arg1	activities					440:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	4	32	from	decrease	1014:1021	arg1	degree					1030:1035	the degree	1026:1035	the degree of esterification of water-soluble polysaccharides at 25 °C	1026:1095	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	33	theme	wall-degrading	695:708	arg1	enzymes					710:716	cell wall-degrading enzymes	690:716	cell wall-degrading enzymes	690:716	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	34	dep	°C.	906:908	arg1	showed					961:966	showed	961:966	showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C	961:1120	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	1	35	theme	postharvest	211:221	arg1	quality					223:229	the postharvest quality	207:229	the postharvest quality	207:229	The metabolism of polysaccharides in the Zizania latifolia cell wall helps maintain the postharvest quality during storage.
35159542	2	36	theme	Z.	253:254	arg1	latifolia					256:264	Fresh Z. latifolia	247:264	Fresh Z. latifolia	247:264	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	3	37	from	decrease	591:598	arg1	propectin					603:611	propectin	603:611	propectin	603:611	The results showed that hardness declined concomitantly with an increase in water-soluble pectin content during storage, as well as with a decrease in propectin and cellulose contents.
35159542	3	37	from	decrease	591:598	arg1	contents					627:634	cellulose contents	617:634	cellulose contents	617:634	The results showed that hardness declined concomitantly with an increase in water-soluble pectin content during storage, as well as with a decrease in propectin and cellulose contents.
35159542	0	38	theme	Polysaccharide	28:41	arg1	Metabolism					43:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	2	39	theme	metabolism-related	414:431	arg1	activities					440:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	4	40	from	°C	1094:1095	arg1	esterification					1040:1053	esterification	1040:1053	esterification of water-soluble polysaccharides at 25 °C	1040:1095	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	40	from	°C	1094:1095	arg1	degree					1030:1035	the degree	1026:1035	the degree of esterification of water-soluble polysaccharides at 25 °C	1026:1095	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	2	41	theme	ingredients	387:397	arg1	activities					440:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	2	42	theme	wall	326:329	arg1	composition					346:356	cell wall polysaccharide composition	321:356	cell wall polysaccharide composition	321:356	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	2	43	theme	active	380:385	arg1	ingredients					387:397	active ingredients	380:397	active ingredients	380:397	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	2	44	theme	cell	321:324	arg1	composition					346:356	cell wall polysaccharide composition	321:356	cell wall polysaccharide composition	321:356	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	4	45	theme	polysaccharides	1072:1086	arg1	esterification					1040:1053	esterification	1040:1053	esterification of water-soluble polysaccharides at 25 °C	1040:1095	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	46	theme	lower	670:674	arg1	polygalacturonase					727:743	polygalacturonase	727:743	polygalacturonase	727:743	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	46	theme	lower	670:674	arg1	cellulase					746:754	cellulase	746:754	cellulase	746:754	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	46	theme	lower	670:674	arg1	β-galactosidase					761:775	β-galactosidase	761:775	β-galactosidase	761:775	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	4	46	theme	lower	670:674	arg1	activities					676:685	lower activities	670:685	lower activities	670:685	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	2	47	theme	Fresh	247:251	arg1	latifolia					256:264	Fresh Z. latifolia	247:264	Fresh Z. latifolia	247:264	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	4	48	theme	water-soluble	1058:1070	arg1	polysaccharides					1072:1086	water-soluble polysaccharides	1058:1086	water-soluble polysaccharides	1058:1086	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	3	49	theme	pectin	542:547	arg1	content					549:555	water-soluble pectin content	528:555	water-soluble pectin content during storage	528:570	The results showed that hardness declined concomitantly with an increase in water-soluble pectin content during storage, as well as with a decrease in propectin and cellulose contents.
35159542	3	50	theme	cellulose	617:625	arg1	contents					627:634	cellulose contents	617:634	cellulose contents	617:634	The results showed that hardness declined concomitantly with an increase in water-soluble pectin content during storage, as well as with a decrease in propectin and cellulose contents.
35159542	2	51	theme	hardness	311:318	arg1	activities					440:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities	307:449	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	4	52	from	polygalacturonase	727:743	arg1	Z.					780:781	Z.	780:781	Z.	780:781	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	0	53	theme	Related	69:75	arg1	Activities					84:93	Related Enzyme Activities	69:93	Related Enzyme Activities	69:93	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	4	54	theme	Correlation	637:647	arg1	analysis					649:656	Correlation analysis	637:656	Correlation analysis	637:656	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
35159542	2	55	theme	cell	359:362	arg1	structure					369:377	cell wall structure	359:377	cell wall structure	359:377	Fresh Z. latifolia was stored at 4 °C and 25 °C to evaluate the hardness, cell wall polysaccharide composition, cell wall structure, active ingredients, and cell wall metabolism-related enzyme activities.
35159542	0	56	theme	Cell	18:21	arg1	Metabolism					43:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism	0:52	Zizania latifolia Cell Wall Polysaccharide Metabolism and Changes of Related Enzyme Activities during Postharvest Storage.
35159542	4	57	dep	Z.	780:781	arg1	latifolia					783:791	Z. latifolia	780:791	Z. latifolia stored at 4 °C	780:806	Correlation analysis showed that lower activities of cell wall-degrading enzymes, such as polygalacturonase, cellulase, and β-galactosidase in Z. latifolia stored at 4 °C, were associated with lighter fiberization and greater hardness, compared with those stored at 25 °C. Additionally, the results of infrared spectroscopy showed that texture softening may be attributed to a decrease in the degree of esterification of water-soluble polysaccharides at 25 °C compared to that at 4 °C.
37259888	0	0	theme	fast	79:82	arg1	sedimentation					84:96	fast sedimentation	79:96	fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942	79:151	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	6	1	from	changes	1074:1080	arg1	polysaccharides					1147:1161	released polysaccharides	1138:1161	released polysaccharides	1138:1161	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	6	1	from	changes	1074:1080	arg1	structures					1098:1107	cell surface structures	1085:1107	cell surface structures	1085:1107	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	5	2	theme	Group	958:962	arg1	F					991:991	the Group 3 alternative sigma factor F	954:991	the Group 3 alternative sigma factor F	954:991	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	4	3	theme	PCC	632:634	arg1	7942-KU					636:642	Synechococcus elongatus PCC 7942-KU	608:642	Synechococcus elongatus PCC 7942-KU	608:642	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	4	3	theme	PCC	632:634	arg1	substrain					666:674	a novel domesticated substrain	645:674	a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942	645:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	5	4	theme	sigma	978:982	arg1	F					991:991	the Group 3 alternative sigma factor F	954:991	the Group 3 alternative sigma factor F	954:991	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	6	5	from	increase	1126:1133	arg1	polysaccharides					1147:1161	released polysaccharides	1138:1161	released polysaccharides	1138:1161	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	6	5	from	increase	1126:1133	arg1	structures					1098:1107	cell surface structures	1085:1107	cell surface structures	1085:1107	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	2	6	theme	microevolutionary	383:399	arg1	events					401:406	multiple microevolutionary events	374:406	multiple microevolutionary events	374:406	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	5	7	theme	higher	778:783	arg1	strengths					791:799	higher ionic strengths	778:799	higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion	778:930	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	0	8	dep	cyanobacterium	105:118	arg1	Synechococcus					120:132	Synechococcus	120:132	Synechococcus	120:132	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	4	9	theme	elongatus	707:715	arg1	7942					721:724	the model cyanobacterium S. elongatus PCC 7942	679:724	the model cyanobacterium S. elongatus PCC 7942	679:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	5	10	theme	ionic	785:789	arg1	strengths					791:799	higher ionic strengths	778:799	higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion	778:930	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	4	11	dep	Synechococcus	608:620	arg1	elongatus					622:630	elongatus	622:630	elongatus	622:630	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	6	12	theme	Further	1033:1039	arg1	analysis					1041:1048	Further analysis	1033:1048	Further analysis	1033:1048	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	7	13	theme	genetic	1315:1321	arg1	targets					1323:1329	genetic targets	1315:1329	genetic targets	1315:1329	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	5	14	theme	cell	1013:1016	arg1	sedimentation					1018:1030	cell sedimentation	1013:1030	cell sedimentation	1013:1030	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	6	15	theme	significant	1062:1072	arg1	changes					1074:1080	significant changes	1062:1080	significant changes in cell surface structures	1062:1107	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	0	16	theme	cyanobacterium	105:118	arg1	7942					148:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	5	17	theme	alternative	966:976	arg1	F					991:991	the Group 3 alternative sigma factor F	954:991	the Group 3 alternative sigma factor F	954:991	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	6	18	theme	released	1138:1145	arg1	polysaccharides					1147:1161	released polysaccharides	1138:1161	released polysaccharides	1138:1161	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	6	19	theme	cell	1085:1088	arg1	structures					1098:1107	cell surface structures	1085:1107	cell surface structures	1085:1107	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	2	20	theme	large	432:436	arg1	number					438:443	a large number	430:443	a large number of 'domesticated' substrains among model cyanobacteria	430:498	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	6	21	theme	surface	1090:1096	arg1	structures					1098:1107	cell surface structures	1085:1107	cell surface structures	1085:1107	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	7	22	theme	cyanobacterial	1401:1414	arg1	harvesting					1416:1425	cost-effective cyanobacterial harvesting	1386:1425	cost-effective cyanobacterial harvesting	1386:1425	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	4	23	theme	S.	704:705	arg1	7942					721:724	the model cyanobacterium S. elongatus PCC 7942	679:724	the model cyanobacterium S. elongatus PCC 7942	679:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	2	24	theme	model	480:484	arg1	cyanobacteria					486:498	model cyanobacteria	480:498	model cyanobacteria	480:498	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	7	25	theme	benthic	1282:1288	arg1	transitions					1290:1300	the planktonic to benthic transitions	1264:1300	the planktonic to benthic transitions	1264:1300	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	7	26	theme	transitions	1290:1300	arg1	determinants					1248:1259	the determinants	1244:1259	the determinants of the planktonic to benthic transitions	1244:1300	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	4	27	theme	7942	721:724	arg1	7942-KU					636:642	Synechococcus elongatus PCC 7942-KU	608:642	Synechococcus elongatus PCC 7942-KU	608:642	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	4	27	theme	7942	721:724	arg1	substrain					666:674	a novel domesticated substrain	645:674	a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942	645:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	0	28	theme	Cell	0:3	arg1	composition					13:23	Cell surface composition	0:23	Cell surface composition	0:23	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	2	29	theme	laboratory	342:351	arg1	culturing					353:361	Prolonged laboratory culturing	332:361	Prolonged laboratory culturing	332:361	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	1	30	theme	ecological	207:216	arg1	Cyanobacteria					154:166	Cyanobacteria	154:166	Cyanobacteria	154:166	Cyanobacteria are photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years.
37259888	1	30	theme	ecological	207:216	arg1	prokaryotes					187:197	photosynthetic prokaryotes	172:197	photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years	172:329	Cyanobacteria are photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years.
37259888	2	31	theme	substrains	463:472	arg1	number					438:443	a large number	430:443	a large number of 'domesticated' substrains among model cyanobacteria	430:498	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	0	32	theme	elongatus	134:142	arg1	7942					148:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	6	33	theme	phenotype	1208:1216	arg1	appearance					1175:1184	the appearance	1171:1184	the appearance of a fast-sedimenting phenotype	1171:1216	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	2	34	theme	Prolonged	332:340	arg1	culturing					353:361	Prolonged laboratory culturing	332:361	Prolonged laboratory culturing	332:361	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	2	35	theme	domesticated	449:460	arg1	substrains					463:472	'domesticated' substrains	448:472	'domesticated' substrains among model cyanobacteria	448:498	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	5	36	theme	whole	890:894	arg1	sequencing					903:912	whole genome sequencing	890:912	whole genome sequencing	890:912	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	4	37	theme	Synechococcus	608:620	arg1	7942-KU					636:642	Synechococcus elongatus PCC 7942-KU	608:642	Synechococcus elongatus PCC 7942-KU	608:642	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	4	37	theme	Synechococcus	608:620	arg1	substrain					666:674	a novel domesticated substrain	645:674	a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942	645:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	1	38	theme	photosynthetic	172:185	arg1	Cyanobacteria					154:166	Cyanobacteria	154:166	Cyanobacteria	154:166	Cyanobacteria are photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years.
37259888	1	38	theme	photosynthetic	172:185	arg1	prokaryotes					187:197	photosynthetic prokaryotes	172:197	photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years	172:329	Cyanobacteria are photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years.
37259888	1	39	theme	biotechnological	222:237	arg1	relevance					239:247	high ecological and biotechnological relevance	202:247	relevance	239:247	Cyanobacteria are photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years.
37259888	4	40	theme	fast-sedimenting	744:759	arg1	phenotype					761:769	a fast-sedimenting phenotype	742:769	a fast-sedimenting phenotype	742:769	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	7	41	dep	transitions	1290:1300	arg1	to					1279:1280	to	1279:1280	to	1279:1280	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	4	42	theme	cyanobacterium	689:702	arg1	7942					721:724	the model cyanobacterium S. elongatus PCC 7942	679:724	the model cyanobacterium S. elongatus PCC 7942	679:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	4	43	theme	model	683:687	arg1	7942					721:724	the model cyanobacterium S. elongatus PCC 7942	679:724	the model cyanobacterium S. elongatus PCC 7942	679:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	4	44	theme	PCC	717:719	arg1	7942					721:724	the model cyanobacterium S. elongatus PCC 7942	679:724	the model cyanobacterium S. elongatus PCC 7942	679:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	1	45	theme	relevance	239:247	arg1	Cyanobacteria					154:166	Cyanobacteria	154:166	Cyanobacteria	154:166	Cyanobacteria are photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years.
37259888	1	45	theme	relevance	239:247	arg1	prokaryotes					187:197	photosynthetic prokaryotes	172:197	photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years	172:329	Cyanobacteria are photosynthetic prokaryotes of high ecological and biotechnological relevance that have been cultivated in laboratories around the world for more than 70 years.
37259888	0	46	theme	released	26:33	arg1	polysaccharides					35:49	released polysaccharides	26:49	released polysaccharides	26:49	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	0	47	theme	PCC	144:146	arg1	7942					148:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	5	48	theme	gene	918:921	arg1	deletion					923:930	gene deletion	918:930	gene deletion	918:930	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	2	49	theme	number	438:443	arg1	events					401:406	multiple microevolutionary events	374:406	multiple microevolutionary events	374:406	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	2	49	theme	number	438:443	arg1	appearance					416:425	the appearance	412:425	the appearance of a large number of 'domesticated' substrains among model cyanobacteria	412:498	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	5	50	dep	strengths	791:799	arg1	increased					824:832	increased	824:832	increased	824:832	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	2	51	theme	multiple	374:381	arg1	events					401:406	multiple microevolutionary events	374:406	multiple microevolutionary events	374:406	Prolonged laboratory culturing has led to multiple microevolutionary events and the appearance of a large number of 'domesticated' substrains among model cyanobacteria.
37259888	7	52	theme	planktonic	1268:1277	arg1	transitions					1290:1300	the planktonic to benthic transitions	1264:1300	the planktonic to benthic transitions	1264:1300	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	3	53	theme	strain	536:541	arg1	domestication					543:555	strain domestication	536:555	strain domestication	536:555	Despite its widespread occurrence, strain domestication is still largely ignored.
37259888	3	54	theme	widespread	513:522	arg1	occurrence					524:533	its widespread occurrence	509:533	its widespread occurrence	509:533	Despite its widespread occurrence, strain domestication is still largely ignored.
37259888	5	55	theme	key	1001:1003	arg1	role					1005:1008	a key role	999:1008	a key role	999:1008	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	4	56	theme	novel	647:651	arg1	7942-KU					636:642	Synechococcus elongatus PCC 7942-KU	608:642	Synechococcus elongatus PCC 7942-KU	608:642	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	4	56	theme	novel	647:651	arg1	substrain					666:674	a novel domesticated substrain	645:674	a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942	645:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	6	57	theme	three-fold	1115:1124	arg1	increase					1126:1133	a three-fold increase	1113:1133	a three-fold increase in released polysaccharides	1113:1161	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	7	58	theme	fast-sedimenting	1343:1358	arg1	strains					1360:1366	fast-sedimenting strains	1343:1366	fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale	1343:1434	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	6	59	theme	fast-sedimenting	1191:1206	arg1	phenotype					1208:1216	a fast-sedimenting phenotype	1189:1216	a fast-sedimenting phenotype	1189:1216	Further analysis showed that significant changes in cell surface structures and a three-fold increase in released polysaccharides lead to the appearance of a fast-sedimenting phenotype.
37259888	5	60	theme	factor	984:989	arg1	F					991:991	the Group 3 alternative sigma factor F	954:991	the Group 3 alternative sigma factor F	954:991	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	5	61	theme	sedimentation	805:817	arg1	rate					819:822	the sedimentation rate	801:822	the sedimentation rate	801:822	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	0	62	from	sedimentation	84:96	arg1	7942					148:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	the cyanobacterium Synechococcus elongatus PCC 7942	101:151	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	5	63	theme	genome	896:901	arg1	sequencing					903:912	whole genome sequencing	890:912	whole genome sequencing	890:912	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	7	64	theme	cost-effective	1386:1399	arg1	harvesting					1416:1425	cost-effective cyanobacterial harvesting	1386:1425	cost-effective cyanobacterial harvesting	1386:1425	This work sheds light on the determinants of the planktonic to benthic transitions and provides genetic targets to generate fast-sedimenting strains that could unlock cost-effective cyanobacterial harvesting at scale.
37259888	0	65	theme	ionic	56:60	arg1	strength					62:69	ionic strength	56:69	ionic strength	56:69	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	5	66	from	sedimentation	854:866	arg1	12 h.					876:880	12 h.	876:880	12 h.	876:880	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	5	67	theme	complete	845:852	arg1	sedimentation					854:866	complete sedimentation	845:866	complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion	845:930	Under higher ionic strengths the sedimentation rate increased leading to complete sedimentation in just 12 h. Through whole genome sequencing and gene deletion, we demonstrated that the Group 3 alternative sigma factor F plays a key role in cell sedimentation.
37259888	0	68	theme	surface	5:11	arg1	composition					13:23	Cell surface composition	0:23	Cell surface composition	0:23	Cell surface composition, released polysaccharides, and ionic strength mediate fast sedimentation in the cyanobacterium Synechococcus elongatus PCC 7942.
37259888	4	69	theme	domesticated	653:664	arg1	7942-KU					636:642	Synechococcus elongatus PCC 7942-KU	608:642	Synechococcus elongatus PCC 7942-KU	608:642	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
37259888	4	69	theme	domesticated	653:664	arg1	substrain					666:674	a novel domesticated substrain	645:674	a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942	645:724	In this work we describe Synechococcus elongatus PCC 7942-KU, a novel domesticated substrain of the model cyanobacterium S. elongatus PCC 7942, which presents a fast-sedimenting phenotype.
35845784	6	0	theme	methylation	884:894	arg1	results					943:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results	852:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results	852:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	3	1	theme	polysaccharides	483:497	arg1	structure					448:456	chemical structure	439:456	chemical structure	439:456	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	3	1	theme	polysaccharides	483:497	arg1	activation					469:478	immune activation	462:478	immune activation	462:478	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	6	2	theme	composition	871:881	arg1	results					943:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results	852:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results	852:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	10	3	from	Tatarinowii	1604:1614	arg1	discovery					1512:1520	the discovery	1508:1520	the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii	1508:1614	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	10	3	from	Tatarinowii	1604:1614	arg1	polysaccharide					1533:1546	natural polysaccharide	1525:1546	natural polysaccharide	1525:1546	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	10	3	from	Tatarinowii	1604:1614	arg1	food					1580:1583	functional food	1569:1583	functional food	1569:1583	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	4	4	theme	G-100	632:636	arg1	chromatography					645:658	DEAE-52 cellulose and Sephadex G-100 column chromatography	601:658	chromatography	645:658	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	10	5	theme	immune	1548:1553	arg1	modulators					1555:1564	immune modulators	1548:1564	immune modulators	1548:1564	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	3	6	from	structure	448:456	arg1	Tatarinowii					518:528	Tatarinowii	518:528	Tatarinowii	518:528	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	0	7	theme	Chemical	0:7	arg1	Structure					9:17	Chemical Structure	0:17	Chemical Structure	0:17	Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii.
35845784	9	8	theme	B	1427:1427	arg1	proliferation					1434:1446	lipopolysaccharide (LPS)-induced B cell proliferation	1394:1446	lipopolysaccharide (LPS)-induced B cell proliferation	1394:1446	RATAPW also enhances ConA-induced T cells and lipopolysaccharide (LPS)-induced B cell proliferation in a dose-dependent effect.
35845784	2	9	theme	essential	295:303	arg1	components					309:318	other essential oil components	289:318	other essential oil components	289:318	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	1	10	theme	heart	179:183	arg1	disease					185:191	coronary heart disease	170:191	coronary heart disease	170:191	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	4	11	theme	DEAE-52	601:607	arg1	cellulose					609:617	DEAE-52 cellulose and Sephadex G-100 column chromatography	601:658	cellulose	609:617	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	10	12	theme	food	1580:1583	arg1	discovery					1512:1520	the discovery	1508:1520	the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii	1508:1614	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	7	13	theme	inhibitor	1058:1066	arg1	assay					1083:1087	inhibitor neutralization assay	1058:1087	inhibitor neutralization assay	1058:1087	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	2	14	theme	large	331:335	arg1	polysaccharides					373:387	polysaccharides	373:387	polysaccharides	373:387	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	2	14	theme	large	331:335	arg1	components					354:363	the large molecular active components	327:363	the large molecular active components such as polysaccharides	327:387	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	8	15	theme	RATAPW	1269:1274	arg1	μg/ml					1260:1264	200 μg/ml	1256:1264	200 μg/ml of RATAPW	1256:1274	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	7	16	theme	macrophage	1158:1167	arg1	production					1135:1144	the TNF-α production	1125:1144	the TNF-α production of RAW264.7 macrophage	1125:1167	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	5	17	theme	RATAPW	825:830	arg1	%					849:849	98.23 ± 0.29%	837:849	98.23 ± 0.29%	837:849	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	5	17	theme	RATAPW	825:830	arg1	contents					813:820	the total carbohydrate contents	790:820	the total carbohydrate contents of RATAPW	790:830	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	6	18	theme	magnetic	909:916	arg1	NMR					929:931	NMR	929:931	NMR	929:931	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	6	18	theme	magnetic	909:916	arg1	resonance					918:926	nuclear magnetic resonance	901:926	nuclear magnetic resonance (NMR) analysis	901:941	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	8	19	theme	%	1292:1292	arg1	rise					1294:1297	a 38.77% rise	1285:1297	a 38.77% rise in the proliferation rate of spleen lymphocytes	1285:1345	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	2	20	theme	small	243:247	arg1	compounds					259:267	small molecular compounds	243:267	small molecular compounds such as asarone and other essential oil components	243:318	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	2	20	theme	small	243:247	arg1	asarone					277:283	asarone	277:283	asarone	277:283	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	2	20	theme	small	243:247	arg1	components					309:318	other essential oil components	289:318	other essential oil components	289:318	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	1	21	theme	Chinese	128:134	arg1	herb					136:139	a traditional Chinese herb	114:139	a traditional Chinese herb used to treat depression and coronary heart disease	114:191	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	1	21	theme	Chinese	128:134	arg1	Tatarinowii					99:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	4	22	theme	Tatarinowii	715:725	arg1	polysaccharide					683:696	alkali extraction polysaccharide	665:696	alkali extraction polysaccharide of Rhizoma Acori Tatarinowii	665:725	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	4	23	theme	alkali	665:670	arg1	polysaccharide					683:696	alkali extraction polysaccharide	665:696	alkali extraction polysaccharide of Rhizoma Acori Tatarinowii	665:725	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	7	24	theme	signaling	1222:1230	arg1	pathway					1232:1238	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	8	25	theme	proliferation	1306:1318	arg1	rate					1320:1323	the proliferation rate	1302:1323	the proliferation rate of spleen lymphocytes	1302:1345	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	3	26	from	Tatarinowii	518:528	arg1	polysaccharides					483:497	polysaccharides	483:497	polysaccharides from Rhizoma Acori Tatarinowii	483:528	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	3	26	from	Tatarinowii	518:528	arg1	structure					448:456	chemical structure	439:456	chemical structure	439:456	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	3	26	from	Tatarinowii	518:528	arg1	activation					469:478	immune activation	462:478	immune activation	462:478	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	8	27	theme	lymphocytes	1335:1345	arg1	rate					1320:1323	the proliferation rate	1302:1323	the proliferation rate of spleen lymphocytes	1302:1345	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	5	28	theme	molecular	740:748	arg1	Da					782:783	2.51 × 104 Da	771:783	2.51 × 104 Da	771:783	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	5	28	theme	molecular	740:748	arg1	weight					750:755	The average molecular weight	728:755	The average molecular weight of RATAPW	728:765	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	7	29	theme	nuclear	1181:1187	arg1	B					1202:1202	the nuclear factor kappa B	1177:1202	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	7	29	theme	nuclear	1181:1187	arg1	NF-κB					1205:1209	NF-κB	1205:1209	NF-κB	1205:1209	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	1	30	theme	Rhizoma	85:91	arg1	herb					136:139	a traditional Chinese herb	114:139	a traditional Chinese herb used to treat depression and coronary heart disease	114:191	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	1	30	theme	Rhizoma	85:91	arg1	Tatarinowii					99:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	5	31	theme	total	794:798	arg1	%					849:849	98.23 ± 0.29%	837:849	98.23 ± 0.29%	837:849	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	5	31	theme	total	794:798	arg1	contents					813:820	the total carbohydrate contents	790:820	the total carbohydrate contents of RATAPW	790:830	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	7	32	theme	RAW264.7	1149:1156	arg1	macrophage					1158:1167	RAW264.7 macrophage	1149:1167	RAW264.7 macrophage	1149:1167	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	6	33	theme	short	1007:1011	arg1	branches					1019:1026	short α-1,6 branches	1007:1026	short α-1,6 branches	1007:1026	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	3	34	theme	immune	462:467	arg1	activation					469:478	immune activation	462:478	immune activation	462:478	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	9	35	theme	ConA-induced	1369:1380	arg1	cells					1384:1388	ConA-induced T cells	1369:1388	ConA-induced T cells	1369:1388	RATAPW also enhances ConA-induced T cells and lipopolysaccharide (LPS)-induced B cell proliferation in a dose-dependent effect.
35845784	10	36	theme	polysaccharide	1533:1546	arg1	discovery					1512:1520	the discovery	1508:1520	the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii	1508:1614	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	5	37	theme	98.23	837:841	arg1	±					843:843	±	843:843	±	843:843	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	0	38	theme	Glucan	46:51	arg1	Structure					9:17	Chemical Structure	0:17	Chemical Structure	0:17	Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii.
35845784	0	38	theme	Glucan	46:51	arg1	Activation					30:39	Immune Activation	23:39	Immune Activation	23:39	Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii.
35845784	8	39	with	Treatment	1241:1249	arg1	μg/ml					1260:1264	200 μg/ml	1256:1264	200 μg/ml of RATAPW	1256:1274	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	2	40	theme	molecular	337:345	arg1	polysaccharides					373:387	polysaccharides	373:387	polysaccharides	373:387	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	2	40	theme	molecular	337:345	arg1	components					354:363	the large molecular active components	327:363	the large molecular active components such as polysaccharides	327:387	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	10	41	theme	functional	1569:1578	arg1	food					1580:1583	functional food	1569:1583	functional food	1569:1583	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	6	42	theme	monosaccharide	856:869	arg1	composition					871:881	monosaccharide composition	856:881	monosaccharide composition	856:881	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	1	43	theme	coronary	170:177	arg1	disease					185:191	coronary heart disease	170:191	coronary heart disease	170:191	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	9	44	theme	-induced	1418:1425	arg1	proliferation					1434:1446	lipopolysaccharide (LPS)-induced B cell proliferation	1394:1446	lipopolysaccharide (LPS)-induced B cell proliferation	1394:1446	RATAPW also enhances ConA-induced T cells and lipopolysaccharide (LPS)-induced B cell proliferation in a dose-dependent effect.
35845784	2	45	from	Studies	194:200	arg1	components					216:225	its active components	205:225	its active components	205:225	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	4	46	theme	Sephadex	623:630	arg1	chromatography					645:658	DEAE-52 cellulose and Sephadex G-100 column chromatography	601:658	chromatography	645:658	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	7	47	theme	neutralization	1068:1081	arg1	assay					1083:1087	inhibitor neutralization assay	1058:1087	inhibitor neutralization assay	1058:1087	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	2	48	theme	oil	305:307	arg1	components					309:318	other essential oil components	289:318	other essential oil components	289:318	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	9	49	theme	cell	1429:1432	arg1	proliferation					1434:1446	lipopolysaccharide (LPS)-induced B cell proliferation	1394:1446	lipopolysaccharide (LPS)-induced B cell proliferation	1394:1446	RATAPW also enhances ConA-induced T cells and lipopolysaccharide (LPS)-induced B cell proliferation in a dose-dependent effect.
35845784	6	50	theme	analysis	934:941	arg1	results					943:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results	852:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results	852:949	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	4	51	theme	column	638:643	arg1	chromatography					645:658	DEAE-52 cellulose and Sephadex G-100 column chromatography	601:658	chromatography	645:658	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	3	52	from	activation	469:478	arg1	Tatarinowii					518:528	Tatarinowii	518:528	Tatarinowii	518:528	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	2	53	theme	other	289:293	arg1	components					309:318	other essential oil components	289:318	other essential oil components	289:318	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	5	54	theme	carbohydrate	800:811	arg1	%					849:849	98.23 ± 0.29%	837:849	98.23 ± 0.29%	837:849	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	5	54	theme	carbohydrate	800:811	arg1	contents					813:820	the total carbohydrate contents	790:820	the total carbohydrate contents of RATAPW	790:830	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	0	55	from	Tatarinowii	72:82	arg1	Structure					9:17	Chemical Structure	0:17	Chemical Structure	0:17	Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii.
35845784	0	55	from	Tatarinowii	72:82	arg1	Activation					30:39	Immune Activation	23:39	Immune Activation	23:39	Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii.
35845784	7	56	theme	Immunofluorescence	1029:1046	arg1	assay					1048:1052	Immunofluorescence assay	1029:1052	Immunofluorescence assay	1029:1052	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	5	57	theme	0.29	845:848	arg1	±					843:843	±	843:843	±	843:843	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	9	58	theme	dose-dependent	1453:1466	arg1	effect					1468:1473	a dose-dependent effect	1451:1473	a dose-dependent effect	1451:1473	RATAPW also enhances ConA-induced T cells and lipopolysaccharide (LPS)-induced B cell proliferation in a dose-dependent effect.
35845784	6	59	theme	resonance	918:926	arg1	analysis					934:941	nuclear magnetic resonance (NMR) analysis	901:941	nuclear magnetic resonance (NMR) analysis	901:941	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	2	60	theme	active	209:214	arg1	components					216:225	its active components	205:225	its active components	205:225	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	6	61	theme	nuclear	901:907	arg1	NMR					929:931	NMR	929:931	NMR	929:931	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	6	61	theme	nuclear	901:907	arg1	resonance					918:926	nuclear magnetic resonance	901:926	nuclear magnetic resonance (NMR) analysis	901:941	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	6	62	with	α-1,4-glucan	989:1000	arg1	branches					1019:1026	short α-1,6 branches	1007:1026	short α-1,6 branches	1007:1026	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	10	63	dep	polysaccharide	1533:1546	arg1	modulators					1555:1564	immune modulators	1548:1564	immune modulators	1548:1564	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	2	64	theme	molecular	249:257	arg1	compounds					259:267	small molecular compounds	243:267	small molecular compounds such as asarone and other essential oil components	243:318	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	2	64	theme	molecular	249:257	arg1	asarone					277:283	asarone	277:283	asarone	277:283	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	2	64	theme	molecular	249:257	arg1	components					309:318	other essential oil components	289:318	other essential oil components	289:318	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	1	65	theme	traditional	116:126	arg1	herb					136:139	a traditional Chinese herb	114:139	a traditional Chinese herb used to treat depression and coronary heart disease	114:191	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	1	65	theme	traditional	116:126	arg1	Tatarinowii					99:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	8	66	theme	38.77	1287:1291	arg1	%					1292:1292	%	1292:1292	%	1292:1292	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	7	67	theme	molecular	1212:1220	arg1	pathway					1232:1238	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	5	68	theme	RATAPW	760:765	arg1	Da					782:783	2.51 × 104 Da	771:783	2.51 × 104 Da	771:783	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	5	68	theme	RATAPW	760:765	arg1	weight					750:755	The average molecular weight	728:755	The average molecular weight of RATAPW	728:765	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	4	69	theme	extraction	672:681	arg1	polysaccharide					683:696	alkali extraction polysaccharide	665:696	alkali extraction polysaccharide of Rhizoma Acori Tatarinowii	665:725	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	7	70	theme	B	1202:1202	arg1	pathway					1232:1238	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	3	71	dep	structure	448:456	arg1	the					435:437	the	435:437	the	435:437	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	5	72	theme	average	732:738	arg1	Da					782:783	2.51 × 104 Da	771:783	2.51 × 104 Da	771:783	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	5	72	theme	average	732:738	arg1	weight					750:755	The average molecular weight	728:755	The average molecular weight of RATAPW	728:765	The average molecular weight of RATAPW was 2.51 × 104 Da, and the total carbohydrate contents of RATAPW were 98.23 ± 0.29%.
35845784	8	73	theme	spleen	1328:1333	arg1	lymphocytes					1335:1345	spleen lymphocytes	1328:1345	spleen lymphocytes	1328:1345	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	7	74	theme	factor	1189:1194	arg1	B					1202:1202	the nuclear factor kappa B	1177:1202	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	7	74	theme	factor	1189:1194	arg1	NF-κB					1205:1209	NF-κB	1205:1209	NF-κB	1205:1209	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	0	75	theme	Immune	23:28	arg1	Activation					30:39	Immune Activation	23:39	Immune Activation	23:39	Chemical Structure and Immune Activation of a Glucan From Rhizoma Acori Tatarinowii.
35845784	8	76	from	rise	1294:1297	arg1	rate					1320:1323	the proliferation rate	1302:1323	the proliferation rate of spleen lymphocytes	1302:1345	Treatment with 200 μg/ml of RATAPW enhanced a 38.77% rise in the proliferation rate of spleen lymphocytes.
35845784	1	77	theme	Acori	93:97	arg1	herb					136:139	a traditional Chinese herb	114:139	a traditional Chinese herb used to treat depression and coronary heart disease	114:191	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	1	77	theme	Acori	93:97	arg1	Tatarinowii					99:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii	85:109	Rhizoma Acori Tatarinowii is a traditional Chinese herb used to treat depression and coronary heart disease.
35845784	3	78	theme	chemical	439:446	arg1	structure					448:456	chemical structure	439:456	chemical structure	439:456	In this study, we aimed to study the chemical structure and immune activation of polysaccharides from Rhizoma Acori Tatarinowii.
35845784	10	79	from	discovery	1512:1520	arg1	Tatarinowii					1604:1614	Tatarinowii	1604:1614	Tatarinowii	1604:1614	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	6	80	theme	α-1,6	1013:1017	arg1	branches					1019:1026	short α-1,6 branches	1007:1026	short α-1,6 branches	1007:1026	The monosaccharide composition, methylation, and nuclear magnetic resonance (NMR) analysis results displayed that the polysaccharide was α-1,4-glucan with short α-1,6 branches.
35845784	7	81	theme	kappa	1196:1200	arg1	B					1202:1202	the nuclear factor kappa B	1177:1202	the nuclear factor kappa B (NF-κB) molecular signaling pathway	1177:1238	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	7	81	theme	kappa	1196:1200	arg1	NF-κB					1205:1209	NF-κB	1205:1209	NF-κB	1205:1209	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	7	82	theme	TNF-α	1129:1133	arg1	production					1135:1144	the TNF-α production	1125:1144	the TNF-α production of RAW264.7 macrophage	1125:1167	Immunofluorescence assay and inhibitor neutralization assay indicated that RATAPW could promote the TNF-α production of RAW264.7 macrophage through the nuclear factor kappa B (NF-κB) molecular signaling pathway.
35845784	9	83	theme	T	1382:1382	arg1	cells					1384:1388	ConA-induced T cells	1369:1388	ConA-induced T cells	1369:1388	RATAPW also enhances ConA-induced T cells and lipopolysaccharide (LPS)-induced B cell proliferation in a dose-dependent effect.
35845784	10	84	theme	natural	1525:1531	arg1	polysaccharide					1533:1546	natural polysaccharide	1525:1546	natural polysaccharide	1525:1546	Our study lays a foundation for the discovery of natural polysaccharide immune modulators or functional food from Rhizoma Acori Tatarinowii.
35845784	2	85	theme	active	347:352	arg1	polysaccharides					373:387	polysaccharides	373:387	polysaccharides	373:387	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	2	85	theme	active	347:352	arg1	components					354:363	the large molecular active components	327:363	the large molecular active components such as polysaccharides	327:387	Studies on its active components mainly focus on small molecular compounds such as asarone and other essential oil components, while the large molecular active components such as polysaccharides are ignored.
35845784	4	86	from	polysaccharide	683:696	arg1	cellulose					609:617	DEAE-52 cellulose and Sephadex G-100 column chromatography	601:658	cellulose	609:617	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35845784	4	86	from	polysaccharide	683:696	arg1	chromatography					645:658	DEAE-52 cellulose and Sephadex G-100 column chromatography	601:658	chromatography	645:658	In this study, a polysaccharide (RATAPW) was isolated and purified by DEAE-52 cellulose and Sephadex G-100 column chromatography from alkali extraction polysaccharide of Rhizoma Acori Tatarinowii.
35928848	0	0	theme	polysaccharide	84:97	arg1	Effect					0:5	Effect	0:5	Effect of ultrasonic degradation on the physicochemical property	0:63	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	0	0	theme	polysaccharide	84:97	arg1	bioactivity					69:79	bioactivity	69:79	bioactivity of polysaccharide	69:97	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	4	1	theme	×	899:899	arg1	GCP					909:911	GCP	909:911	GCP	909:911	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	4	1	theme	×	899:899	arg1	Da					905:906	8.093 × 104 Da	893:906	8.093 × 104 Da (GCP)	893:912	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	5	2	theme	ABTS	1123:1126	arg1	radical					1129:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	1	3	theme	enzymatic	158:166	arg1	process					168:174	the enzymatic process	154:174	the enzymatic process	154:174	Similar to the enzymatic process, there might also be an active fragment in polysaccharides, how to obtain is important for investigating the bioactivity and pharmacological mechanism of polysaccharides.
35928848	5	4	theme	GCP	1248:1250	arg1	GCP-F2					1263:1268	GCP < GCP-F1 < GCP-F2	1248:1268	GCP < GCP-F1 < GCP-F2	1248:1268	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	2	5	theme	ultrasonic	506:515	arg1	treatment					517:525	ultrasonic treatment	506:525	ultrasonic treatment	506:525	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	4	6	theme	8.093	893:897	arg1	GCP					909:911	GCP	909:911	GCP	909:911	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	4	6	theme	8.093	893:897	arg1	Da					905:906	8.093 × 104 Da	893:906	8.093 × 104 Da (GCP)	893:912	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	6	7	theme	antibacterial	1351:1363	arg1	activity					1365:1372	the antibacterial activity	1347:1372	the antibacterial activity of GCP-F1	1347:1382	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	5	8	theme	<	1252:1252	arg1	GCP-F2					1263:1268	GCP < GCP-F1 < GCP-F2	1248:1268	GCP < GCP-F1 < GCP-F2	1248:1268	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	5	9	theme	hydroxyl	1207:1214	arg1	radical					1216:1222	hydroxyl radical	1207:1222	hydroxyl radical	1207:1222	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	2	10	theme	GCP-F1	560:565	arg1	fragments					547:555	two polysaccharide fragments	528:555	two polysaccharide fragments of GCP-F1 and GCP-F2	528:576	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	0	11	from	Effect	0:5	arg1	property					56:63	the physicochemical property	36:63	the physicochemical property	36:63	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	5	12	theme	GCP-F1	1254:1259	arg1	GCP-F2					1263:1268	GCP < GCP-F1 < GCP-F2	1248:1268	GCP < GCP-F1 < GCP-F2	1248:1268	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	4	13	theme	×	942:942	arg1	Da					948:949	1.027 × 104 Da	936:949	1.027 × 104 Da	936:949	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	5	14	theme	3-ethylbenzothiazoline-6-sulfonic	1083:1115	arg1	radical					1129:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	2	15	theme	Gynostemma	360:369	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	15	theme	Gynostemma	360:369	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	0	16	theme	globosum	122:129	arg1	CGMCC					131:135	Chaetomium globosum CGMCC 6882	111:140	Chaetomium globosum CGMCC 6882	111:140	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	5	17	theme	acid	1117:1120	arg1	radical					1129:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	4	18	theme	GCP-F2	871:876	arg1	weights					849:855	weight-average molecular weights	824:855	weight-average molecular weights of GCP-F1 and GCP-F2	824:876	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	6	19	theme	further	1459:1465	arg1	treatment					1478:1486	further ultrasound treatment	1459:1486	further ultrasound treatment	1459:1486	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	2	20	theme	CGMCC	422:426	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	20	theme	CGMCC	422:426	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	0	21	theme	Chaetomium	111:120	arg1	CGMCC					131:135	Chaetomium globosum CGMCC 6882	111:140	Chaetomium globosum CGMCC 6882	111:140	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	7	22	theme	GCP-F1	1548:1553	arg1	activities					1534:1543	the antioxidant and antibacterial activities	1500:1543	the antioxidant and antibacterial activities of GCP-F1 and GCP-F2	1500:1564	Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
35928848	2	23	theme	globosum	413:420	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	23	theme	globosum	413:420	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	6	24	theme	antibacterial	1431:1443	arg1	activity					1445:1452	its antibacterial activity	1427:1452	its antibacterial activity	1427:1452	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	3	25	theme	glucuronic	742:751	arg1	arabinose					690:698	arabinose	690:698	arabinose	690:698	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	3	25	theme	glucuronic	742:751	arg1	acid					753:756	glucuronic acid	742:756	glucuronic acid	742:756	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	5	26	theme	scavenging	975:984	arg1	assays					986:991	In vitro scavenging assays	966:991	In vitro scavenging assays	966:991	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	4	27	theme	1.027	936:940	arg1	Da					948:949	1.027 × 104 Da	936:949	1.027 × 104 Da	936:949	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	2	28	theme	Chaetomium	402:411	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	28	theme	Chaetomium	402:411	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	6	29	theme	ultrasonic	1314:1323	arg1	degradation					1325:1335	ultrasonic degradation	1314:1335	ultrasonic degradation	1314:1335	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	7	30	theme	antioxidant	1504:1514	arg1	activities					1534:1543	the antioxidant and antibacterial activities	1500:1543	the antioxidant and antibacterial activities of GCP-F1 and GCP-F2	1500:1564	Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
35928848	7	31	theme	molecular	1609:1617	arg1	weights					1619:1625	their molecular weights	1603:1625	their molecular weights	1603:1625	Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
35928848	2	32	theme	fungus	395:400	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	32	theme	fungus	395:400	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	0	33	theme	degradation	21:31	arg1	Effect					0:5	Effect	0:5	Effect of ultrasonic degradation on the physicochemical property	0:63	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	0	33	theme	degradation	21:31	arg1	bioactivity					69:79	bioactivity	69:79	bioactivity of polysaccharide	69:97	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	5	34	theme	superoxide	1184:1193	arg1	anions					1195:1200	superoxide anions	1184:1200	superoxide anions	1184:1200	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	4	35	theme	×	923:923	arg1	Da					929:930	3.158 × 104 Da	917:930	3.158 × 104 Da	917:930	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	6	36	theme	GCP-F1	1377:1382	arg1	activity					1365:1372	the antibacterial activity	1347:1372	the antibacterial activity of GCP-F1	1347:1382	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	2	37	theme	Combined	459:466	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	37	theme	Combined	459:466	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	0	38	theme	ultrasonic	10:19	arg1	degradation					21:31	ultrasonic degradation	10:31	ultrasonic degradation	10:31	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	5	39	dep	In	966:967	arg1	vitro					969:973	vitro	969:973	vitro	969:973	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	4	40	theme	3.158	917:921	arg1	Da					929:930	3.158 × 104 Da	917:930	3.158 × 104 Da	917:930	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	3	41	theme	arabinose	690:698	arg1	composition					675:685	the same monosaccharide composition	651:685	the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid	651:756	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	3	42	theme	Physicochemical	593:607	arg1	results					609:615	Physicochemical results	593:615	Physicochemical results	593:615	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	2	43	theme	[Genistein	448:457	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	43	theme	[Genistein	448:457	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	5	44	theme	2,2-diphenyl-1-picrylhydrazyl	1138:1166	arg1	radical					1175:1181	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical	1138:1181	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical	1138:1181	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	1	45	theme	bioactivity	285:295	arg1	mechanism					317:325	the bioactivity and pharmacological mechanism	281:325	the bioactivity and pharmacological mechanism of polysaccharides	281:344	Similar to the enzymatic process, there might also be an active fragment in polysaccharides, how to obtain is important for investigating the bioactivity and pharmacological mechanism of polysaccharides.
35928848	2	46	theme	GCP-F2	571:576	arg1	fragments					547:555	two polysaccharide fragments	528:555	two polysaccharide fragments of GCP-F1 and GCP-F2	528:576	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	1	47	theme	active	200:205	arg1	fragment					207:214	an active fragment	197:214	an active fragment in polysaccharides	197:233	Similar to the enzymatic process, there might also be an active fragment in polysaccharides, how to obtain is important for investigating the bioactivity and pharmacological mechanism of polysaccharides.
35928848	6	48	theme	Meanwhile	1271:1279	arg1	tests					1296:1300	Meanwhile, antibacterial tests	1271:1300	Meanwhile, antibacterial tests	1271:1300	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	2	49	theme	polysaccharide	433:446	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	49	theme	polysaccharide	433:446	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	3	50	theme	molar	801:805	arg1	ratios					807:812	slightly different molar ratios	782:812	slightly different molar ratios	782:812	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	0	51	from	bioactivity	69:79	arg1	property					56:63	the physicochemical property	36:63	the physicochemical property	36:63	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	4	52	theme	molecular	839:847	arg1	weights					849:855	weight-average molecular weights	824:855	weight-average molecular weights of GCP-F1 and GCP-F2	824:876	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	2	53	theme	polysaccharide	532:545	arg1	fragments					547:555	two polysaccharide fragments	528:555	two polysaccharide fragments of GCP-F1 and GCP-F2	528:576	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	5	54	contain	had	1028:1030	arg1	GCP-F2					1021:1026	GCP-F2	1021:1026	GCP-F2	1021:1026	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	5	54	contain	had	1028:1030	arg1	GCP-F1					1010:1015	GCP-F1	1010:1015	GCP-F1	1010:1015	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	5	54	contain	had	1028:1030	arg2	activity					1051:1058	higher antioxidant activity	1032:1058	higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical	1032:1222	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	1	55	theme	pharmacological	301:315	arg1	mechanism					317:325	the bioactivity and pharmacological mechanism	281:325	the bioactivity and pharmacological mechanism of polysaccharides	281:344	Similar to the enzymatic process, there might also be an active fragment in polysaccharides, how to obtain is important for investigating the bioactivity and pharmacological mechanism of polysaccharides.
35928848	4	56	theme	GCP-F1	860:865	arg1	weights					849:855	weight-average molecular weights	824:855	weight-average molecular weights of GCP-F1 and GCP-F2	824:876	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	1	57	from	fragment	207:214	arg1	polysaccharides					219:233	polysaccharides	219:233	polysaccharides	219:233	Similar to the enzymatic process, there might also be an active fragment in polysaccharides, how to obtain is important for investigating the bioactivity and pharmacological mechanism of polysaccharides.
35928848	0	58	theme	physicochemical	40:54	arg1	property					56:63	the physicochemical property	36:63	the physicochemical property	36:63	Effect of ultrasonic degradation on the physicochemical property and bioactivity of polysaccharide produced by Chaetomium globosum CGMCC 6882.
35928848	7	59	theme	antibacterial	1520:1532	arg1	activities					1534:1543	the antioxidant and antibacterial activities	1500:1543	the antioxidant and antibacterial activities of GCP-F1 and GCP-F2	1500:1564	Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
35928848	7	60	from	Changes	1489:1495	arg1	activities					1534:1543	the antioxidant and antibacterial activities	1500:1543	the antioxidant and antibacterial activities of GCP-F1 and GCP-F2	1500:1564	Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
35928848	5	61	theme	2,2'-azinobis-	1068:1081	arg1	radical					1129:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical	1068:1135	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	5	62	theme	<	1261:1261	arg1	GCP-F2					1263:1268	GCP < GCP-F1 < GCP-F2	1248:1268	GCP < GCP-F1 < GCP-F2	1248:1268	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	3	63	theme	different	791:799	arg1	ratios					807:812	slightly different molar ratios	782:812	slightly different molar ratios	782:812	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	5	64	theme	In	966:967	arg1	assays					986:991	In vitro scavenging assays	966:991	In vitro scavenging assays	966:991	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	3	65	theme	same	655:658	arg1	composition					675:685	the same monosaccharide composition	651:685	the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid	651:756	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	5	66	theme	higher	1032:1037	arg1	activity					1051:1058	higher antioxidant activity	1032:1058	higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical	1032:1222	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	3	67	theme	monosaccharide	660:673	arg1	composition					675:685	the same monosaccharide composition	651:685	the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid	651:756	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	6	68	dep	Meanwhile	1271:1279	arg1	antibacterial					1282:1294	antibacterial	1282:1294	antibacterial	1282:1294	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	3	69	contain	had	647:649	arg2	composition					675:685	the same monosaccharide composition	651:685	the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid	651:756	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	3	69	contain	had	647:649	arg1	GCP-F2					640:645	GCP-F2	640:645	GCP-F2	640:645	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	3	69	contain	had	647:649	arg1	GCP-F1					629:634	GCP-F1	629:634	GCP-F1	629:634	Physicochemical results showed that GCP-F1 and GCP-F2 had the same monosaccharide composition of arabinose, galactose, glucose, xylose, mannose, and glucuronic acid as compared to GCP with slightly different molar ratios.
35928848	5	70	theme	antioxidant	1039:1049	arg1	activity					1051:1058	higher antioxidant activity	1032:1058	higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical	1032:1222	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35928848	4	71	theme	weight-average	824:837	arg1	weights					849:855	weight-average molecular weights	824:855	weight-average molecular weights of GCP-F1 and GCP-F2	824:876	However, weight-average molecular weights of GCP-F1 and GCP-F2 decreased from 8.093 × 104 Da (GCP) to 3.158 × 104 Da and 1.027 × 104 Da, respectively.
35928848	7	72	theme	weights	1619:1625	arg1	variation					1590:1598	the variation	1586:1598	the variation of their molecular weights	1586:1625	Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
35928848	2	73	theme	endophytic	384:393	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	73	theme	endophytic	384:393	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	7	74	theme	GCP-F2	1559:1564	arg1	activities					1534:1543	the antioxidant and antibacterial activities	1500:1543	the antioxidant and antibacterial activities of GCP-F1 and GCP-F2	1500:1564	Changes in the antioxidant and antibacterial activities of GCP-F1 and GCP-F2 might be related to the variation of their molecular weights.
35928848	1	75	theme	polysaccharides	330:344	arg1	mechanism					317:325	the bioactivity and pharmacological mechanism	281:325	the bioactivity and pharmacological mechanism of polysaccharides	281:344	Similar to the enzymatic process, there might also be an active fragment in polysaccharides, how to obtain is important for investigating the bioactivity and pharmacological mechanism of polysaccharides.
35928848	2	76	theme	pentaphyllum	371:382	arg1	GCP					484:486	GCP	484:486	GCP	484:486	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	2	76	theme	pentaphyllum	371:382	arg1	Polysaccharide					468:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide	358:481	a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)]	358:488	Presently, a Gynostemma pentaphyllum endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide [Genistein Combined Polysaccharide (GCP)] was degraded by ultrasonic treatment, two polysaccharide fragments of GCP-F1 and GCP-F2 were obtained.
35928848	6	77	theme	ultrasound	1467:1476	arg1	treatment					1478:1486	further ultrasound treatment	1459:1486	further ultrasound treatment	1459:1486	Meanwhile, antibacterial tests showed that ultrasonic degradation increased the antibacterial activity of GCP-F1 as compared to GCP, but GCP-F2 almost lost its antibacterial activity with further ultrasound treatment.
35928848	5	78	theme	DPPH	1169:1172	arg1	radical					1175:1181	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical	1138:1181	2,2-diphenyl-1-picrylhydrazyl (DPPH) radical	1138:1181	In vitro scavenging assays illustrated that GCP-F1 and GCP-F2 had higher antioxidant activity against 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, superoxide anions, and hydroxyl radical than GCP, the order was GCP < GCP-F1 < GCP-F2.
35567323	0	0	theme	extract	81:87	arg1	evaluation					56:65	evaluation	56:65	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.	0:130	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	4	1	theme	flocculation	667:678	arg1	rate					680:683	flocculation rate	667:683	flocculation rate	667:683	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	2	used	used	551:554	arg2	FE					543:544	FE	543:544	FE	543:544	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	2	used	used	551:554	arg2	extract					534:540	the fish waste extract	519:540	the fish waste extract (FE)	519:545	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	3	theme	similar	685:691	arg1	level					658:662	a significant level	644:662	a significant level of flocculation rate similar to the commercial nitrogen sources	644:726	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	6	4	theme	FTIR	975:978	arg1	analysis					980:987	The FTIR analysis	971:987	The FTIR analysis of the bioflocculant	971:1008	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	0	5	theme	waste	75:79	arg1	extract					81:87	fish waste extract	70:87	fish waste extract	70:87	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	4	6	theme	fish	523:526	arg1	FE					543:544	FE	543:544	FE	543:544	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	6	theme	fish	523:526	arg1	extract					534:540	the fish waste extract	519:540	the fish waste extract (FE)	519:545	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	7	theme	rate	680:683	arg1	level					658:662	a significant level	644:662	a significant level of flocculation rate similar to the commercial nitrogen sources	644:726	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	3	8	theme	remarkable	366:375	arg1	rates					390:394	remarkable flocculation rates	366:394	remarkable flocculation rates	366:394	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	8	9	theme	kaolin	1386:1391	arg1	wastewater					1393:1402	the kaolin wastewater	1382:1402	the kaolin wastewater	1382:1402	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	5	10	theme	suitable	925:932	arg1	levels					934:939	the suitable levels	921:939	the suitable levels for bioflocculant production	921:968	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	5	10	theme	suitable	925:932	arg1	glucose					856:862	glucose	856:862	glucose	856:862	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	5	10	theme	suitable	925:932	arg1	L-1					874:876	3.247 g L-1	866:876	3.247 g L-1	866:876	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	6	11	theme	related	1126:1132	arg1	components					1115:1124	the fluorescence components	1098:1124	the fluorescence components related to the proteins and humic acids	1098:1164	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	3	12	theme	flocculation	377:388	arg1	rates					390:394	remarkable flocculation rates	366:394	remarkable flocculation rates	366:394	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	4	13	theme	nitrogen	610:617	arg1	productivity					571:582	the productivity	567:582	the productivity of the bioflocculant	567:603	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	13	theme	nitrogen	610:617	arg1	supplement					619:628	a nitrogen supplement	608:628	a nitrogen supplement	608:628	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	10	14	dep	Cr	1686:1687	arg1	VI					1689:1690	VI	1689:1690	VI	1689:1690	The Cr(VI) removal efficiency of the bioflocculant showed that ∼35% of heavy metal is trapped into flocks during the flocculation.
35567323	10	14	dep	Cr	1686:1687	arg1	efficiency					1701:1710	removal efficiency	1693:1710	The Cr(VI) removal efficiency of the bioflocculant	1682:1731	The Cr(VI) removal efficiency of the bioflocculant showed that ∼35% of heavy metal is trapped into flocks during the flocculation.
35567323	5	15	theme	0.5 g	884:888	arg1	L-1					890:892	0.5 g L-1	884:892	0.5 g L-1	884:892	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	3	16	theme	nitrogen	488:495	arg1	sources					497:503	many commercial nitrogen sources	472:503	many commercial nitrogen sources	472:503	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	0	17	theme	flocculation	106:117	arg1	performance					119:129	flocculation performance	106:129	flocculation performance	106:129	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	8	18	theme	pH	1481:1482	arg1	range					1484:1488	the pH range	1477:1488	the pH range	1477:1488	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	8	18	theme	pH	1481:1482	arg1	broad					1494:1498	broad	1494:1498	broad	1494:1498	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	3	19	theme	many	472:475	arg1	sources					497:503	many commercial nitrogen sources	472:503	many commercial nitrogen sources	472:503	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	6	20	theme	bioflocculant	996:1008	arg1	analysis					980:987	The FTIR analysis	971:987	The FTIR analysis of the bioflocculant	971:1008	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	6	21	theme	functional	1021:1030	arg1	groups					1032:1037	the functional groups	1017:1037	the functional groups related to the polysaccharides	1017:1068	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	5	22	theme	Box-Behnken	733:743	arg1	experiments					745:755	The Box-Behnken experiments	729:755	The Box-Behnken experiments	729:755	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	0	23	from	pneumoniae	44:53	arg1	Production					0:9	Production	0:9	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.	0:130	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	9	24	theme	trivalent	1634:1642	arg1	cations					1644:1650	the trivalent cations	1630:1650	the trivalent cations	1630:1650	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	2	25	theme	Klebsiella	278:287	arg1	pneumoniae					289:298	Klebsiella pneumoniae	278:298	Klebsiella pneumoniae	278:298	The isolated strain was identified as Klebsiella pneumoniae by using 16S rRNA gene sequencing.
35567323	2	25	theme	Klebsiella	278:287	arg1	strain					253:258	The isolated strain	240:258	The isolated strain	240:258	The isolated strain was identified as Klebsiella pneumoniae by using 16S rRNA gene sequencing.
35567323	7	26	theme	bioflocculant	1202:1214	arg1	composition					1183:1193	The biochemical composition	1167:1193	The biochemical composition of the bioflocculant	1167:1214	The biochemical composition of the bioflocculant was identified as polysaccharides (24.36 ± 1.5%) and protein (12.15 ± 0.2%).
35567323	7	26	theme	bioflocculant	1202:1214	arg1	polysaccharides					1234:1248	polysaccharides	1234:1248	polysaccharides (24.36 ± 1.5%)	1234:1263	The biochemical composition of the bioflocculant was identified as polysaccharides (24.36 ± 1.5%) and protein (12.15 ± 0.2%).
35567323	7	26	theme	bioflocculant	1202:1214	arg1	protein					1269:1275	protein	1269:1275	protein (12.15 ± 0.2%)	1269:1290	The biochemical composition of the bioflocculant was identified as polysaccharides (24.36 ± 1.5%) and protein (12.15 ± 0.2%).
35567323	3	27	dep	sources	423:429	arg1	sucrose					447:453	sucrose	447:453	sucrose	447:453	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	3	27	dep	sources	423:429	arg1	glucose					438:444	glucose	438:444	glucose	438:444	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	3	27	dep	sources	423:429	arg1	sources					423:429	the carbon sources namely glucose, sucrose and lactose	412:465	the carbon sources namely glucose, sucrose and lactose	412:465	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	3	27	dep	sources	423:429	arg1	lactose					459:465	lactose	459:465	lactose	459:465	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	3	28	theme	K.	339:340	arg1	UKD24					353:357	The K. pneumoniae UKD24	335:357	The K. pneumoniae UKD24	335:357	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	5	29	theme	1	914:914	arg1	%					915:915	%	915:915	%	915:915	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	5	30	theme	bioflocculant	809:821	arg1	production					823:832	bioflocculant production	809:832	bioflocculant production	809:832	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	9	31	theme	cation	1513:1518	arg1	tests					1531:1535	The cation dependency tests	1509:1535	The cation dependency tests	1509:1535	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	4	32	theme	commercial	700:709	arg1	sources					720:726	the commercial nitrogen sources	696:726	the commercial nitrogen sources	696:726	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	2	33	theme	gene	318:321	arg1	sequencing					323:332	16S rRNA gene sequencing	309:332	16S rRNA gene sequencing	309:332	The isolated strain was identified as Klebsiella pneumoniae by using 16S rRNA gene sequencing.
35567323	8	34	theme	bioflocculant	1330:1342	arg1	conditions					1312:1321	The tested optimum conditions	1293:1321	The tested optimum conditions of the bioflocculant to induce flocculation	1293:1365	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	4	35	theme	significant	646:656	arg1	level					658:662	a significant level	644:662	a significant level of flocculation rate similar to the commercial nitrogen sources	644:726	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	8	36	theme	optimum	1427:1433	arg1	dosage					1435:1440	the optimum dosage	1423:1440	the optimum dosage of the flocculant	1423:1458	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	8	36	theme	optimum	1427:1433	arg1	L-1					1469:1471	5 mg L-1	1464:1471	5 mg L-1	1464:1471	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	2	37	theme	rRNA	313:316	arg1	sequencing					323:332	16S rRNA gene sequencing	309:332	16S rRNA gene sequencing	309:332	The isolated strain was identified as Klebsiella pneumoniae by using 16S rRNA gene sequencing.
35567323	10	38	theme	metal	1759:1763	arg1	%					1748:1748	∼35%	1745:1748	∼35% of heavy metal	1745:1763	The Cr(VI) removal efficiency of the bioflocculant showed that ∼35% of heavy metal is trapped into flocks during the flocculation.
35567323	10	38	theme	metal	1759:1763	arg1	metal					1759:1763	metal	1759:1763	metal	1759:1763	The Cr(VI) removal efficiency of the bioflocculant showed that ∼35% of heavy metal is trapped into flocks during the flocculation.
35567323	0	39	theme	bioflocculant	14:26	arg1	Production					0:9	Production	0:9	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.	0:130	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	5	40	theme	%	915:915	arg1	FE					879:880	FE	879:880	FE - 0.5 g L-1 and inoculum size - 1%	879:915	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	5	40	theme	%	915:915	arg1	L-1					874:876	3.247 g L-1	866:876	3.247 g L-1	866:876	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	2	41	theme	16S	309:311	arg1	sequencing					323:332	16S rRNA gene sequencing	309:332	16S rRNA gene sequencing	309:332	The isolated strain was identified as Klebsiella pneumoniae by using 16S rRNA gene sequencing.
35567323	1	42	theme	domestic	207:214	arg1	sewage					216:221	domestic sewage	207:221	the domestic sewage treatment plant	203:237	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	6	43	theme	fluorescence	1102:1113	arg1	components					1115:1124	the fluorescence components	1098:1124	the fluorescence components related to the proteins and humic acids	1098:1164	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	8	44	theme	flocculant	1449:1458	arg1	dosage					1435:1440	the optimum dosage	1423:1440	the optimum dosage of the flocculant	1423:1458	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	8	44	theme	flocculant	1449:1458	arg1	L-1					1469:1471	5 mg L-1	1464:1471	5 mg L-1	1464:1471	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	2	45	theme	isolated	244:251	arg1	pneumoniae					289:298	Klebsiella pneumoniae	278:298	Klebsiella pneumoniae	278:298	The isolated strain was identified as Klebsiella pneumoniae by using 16S rRNA gene sequencing.
35567323	2	45	theme	isolated	244:251	arg1	strain					253:258	The isolated strain	240:258	The isolated strain	240:258	The isolated strain was identified as Klebsiella pneumoniae by using 16S rRNA gene sequencing.
35567323	6	46	theme	related	1039:1045	arg1	groups					1032:1037	the functional groups	1017:1037	the functional groups related to the polysaccharides	1017:1068	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	1	47	theme	sewage	216:221	arg1	plant					233:237	the domestic sewage treatment plant	203:237	the domestic sewage treatment plant	203:237	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	0	48	theme	Klebsiella	33:42	arg1	pneumoniae					44:53	Klebsiella pneumoniae	33:53	Klebsiella pneumoniae	33:53	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	1	49	theme	treatment	223:231	arg1	plant					233:237	the domestic sewage treatment plant	203:237	the domestic sewage treatment plant	203:237	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	5	50	theme	optimal	786:792	arg1	conditions					794:803	the optimal conditions	782:803	the optimal conditions for bioflocculant production	782:832	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	4	51	theme	waste	528:532	arg1	FE					543:544	FE	543:544	FE	543:544	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	51	theme	waste	528:532	arg1	extract					534:540	the fish waste extract	519:540	the fish waste extract (FE)	519:545	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	1	52	attach	isolated	189:196	arg2	UKD24					179:183	UKD24	179:183	UKD24	179:183	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	1	52	attach	isolated	189:196	arg1	plant					233:237	the domestic sewage treatment plant	203:237	the domestic sewage treatment plant	203:237	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	1	52	attach	isolated	189:196	arg2	strain					170:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	8	53	theme	5 mg	1464:1467	arg1	dosage					1435:1440	the optimum dosage	1423:1440	the optimum dosage of the flocculant	1423:1458	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	8	53	theme	5 mg	1464:1467	arg1	L-1					1469:1471	5 mg L-1	1464:1471	5 mg L-1	1464:1471	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	3	54	theme	carbon	416:421	arg1	sucrose					447:453	sucrose	447:453	sucrose	447:453	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	3	54	theme	carbon	416:421	arg1	glucose					438:444	glucose	438:444	glucose	438:444	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	3	54	theme	carbon	416:421	arg1	sources					423:429	the carbon sources namely glucose, sucrose and lactose	412:465	the carbon sources namely glucose, sucrose and lactose	412:465	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	3	54	theme	carbon	416:421	arg1	lactose					459:465	lactose	459:465	lactose	459:465	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	7	55	theme	biochemical	1171:1181	arg1	composition					1183:1193	The biochemical composition	1167:1193	The biochemical composition of the bioflocculant	1167:1214	The biochemical composition of the bioflocculant was identified as polysaccharides (24.36 ± 1.5%) and protein (12.15 ± 0.2%).
35567323	7	55	theme	biochemical	1171:1181	arg1	polysaccharides					1234:1248	polysaccharides	1234:1248	polysaccharides (24.36 ± 1.5%)	1234:1263	The biochemical composition of the bioflocculant was identified as polysaccharides (24.36 ± 1.5%) and protein (12.15 ± 0.2%).
35567323	7	55	theme	biochemical	1171:1181	arg1	protein					1269:1275	protein	1269:1275	protein (12.15 ± 0.2%)	1269:1290	The biochemical composition of the bioflocculant was identified as polysaccharides (24.36 ± 1.5%) and protein (12.15 ± 0.2%).
35567323	8	56	theme	tested	1297:1302	arg1	conditions					1312:1321	The tested optimum conditions	1293:1321	The tested optimum conditions of the bioflocculant to induce flocculation	1293:1365	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	5	57	theme	inoculum	898:905	arg1	size					907:910	inoculum size	898:910	inoculum size	898:910	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	10	58	theme	bioflocculant	1719:1731	arg1	Cr					1686:1687	The Cr(VI) removal efficiency of the bioflocculant	1682:1731	The Cr(VI) removal efficiency of the bioflocculant	1682:1731	The Cr(VI) removal efficiency of the bioflocculant showed that ∼35% of heavy metal is trapped into flocks during the flocculation.
35567323	9	59	theme	moderate	1659:1666	arg1	flocculation					1668:1679	moderate flocculation	1659:1679	moderate flocculation	1659:1679	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	9	60	theme	monovalent	1555:1564	arg1	cations					1579:1585	the monovalent and divalent cations	1551:1585	the monovalent and divalent cations	1551:1585	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	9	60	theme	monovalent	1555:1564	arg1	suitable					1598:1605	suitable	1598:1605	suitable	1598:1605	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	9	61	theme	divalent	1570:1577	arg1	cations					1579:1585	the monovalent and divalent cations	1551:1585	the monovalent and divalent cations	1551:1585	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	9	61	theme	divalent	1570:1577	arg1	suitable					1598:1605	suitable	1598:1605	suitable	1598:1605	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	3	62	theme	commercial	477:486	arg1	sources					497:503	many commercial nitrogen sources	472:503	many commercial nitrogen sources	472:503	The K. pneumoniae UKD24 showed remarkable flocculation rates when grown with the carbon sources namely glucose, sucrose and lactose, and many commercial nitrogen sources.
35567323	5	63	dep	FE	879:880	arg1	L-1					890:892	0.5 g L-1	884:892	0.5 g L-1	884:892	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	5	63	dep	FE	879:880	arg1	size					907:910	inoculum size	898:910	inoculum size	898:910	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	4	64	theme	bioflocculant	591:603	arg1	productivity					571:582	the productivity	567:582	the productivity of the bioflocculant	567:603	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	4	64	theme	bioflocculant	591:603	arg1	supplement					619:628	a nitrogen supplement	608:628	a nitrogen supplement	608:628	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	5	65	theme	bioflocculant	945:957	arg1	production					959:968	bioflocculant production	945:968	bioflocculant production	945:968	The Box-Behnken experiments were designed to predict the optimal conditions for bioflocculant production and it suggested that glucose - 3.247 g L-1, FE - 0.5 g L-1 and inoculum size - 1% are the suitable levels for bioflocculant production.
35567323	1	66	theme	bioflocculant	136:148	arg1	strain					170:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	8	67	theme	optimum	1304:1310	arg1	conditions					1312:1321	The tested optimum conditions	1293:1321	The tested optimum conditions of the bioflocculant to induce flocculation	1293:1365	The tested optimum conditions of the bioflocculant to induce flocculation were tested in the kaolin wastewater and it showed that the optimum dosage of the flocculant was 5 mg L-1 and the pH range was broad as 5-10.
35567323	4	68	theme	nitrogen	711:718	arg1	sources					720:726	the commercial nitrogen sources	696:726	the commercial nitrogen sources	696:726	Furthermore, the fish waste extract (FE) was used to enhance the productivity of the bioflocculant as a nitrogen supplement and it showed a significant level of flocculation rate similar to the commercial nitrogen sources.
35567323	0	69	theme	fish	70:73	arg1	extract					81:87	fish waste extract	70:87	fish waste extract	70:87	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	6	70	theme	EEM	1078:1080	arg1	analysis					1082:1089	the EEM analysis	1074:1089	the EEM analysis	1074:1089	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	1	71	theme	producing	150:158	arg1	strain					170:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
35567323	6	72	theme	humic	1154:1158	arg1	acids					1160:1164	humic acids	1154:1164	humic acids	1154:1164	The FTIR analysis of the bioflocculant showed the functional groups related to the polysaccharides and the EEM analysis showed the fluorescence components related to the proteins and humic acids.
35567323	10	73	theme	removal	1693:1699	arg1	efficiency					1701:1710	removal efficiency	1693:1710	The Cr(VI) removal efficiency of the bioflocculant	1682:1731	The Cr(VI) removal efficiency of the bioflocculant showed that ∼35% of heavy metal is trapped into flocks during the flocculation.
35567323	0	74	dep	Production	0:9	arg1	evaluation					56:65	evaluation	56:65	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.	0:130	Production of bioflocculant from Klebsiella pneumoniae: evaluation of fish waste extract as substrate and flocculation performance.
35567323	9	75	theme	dependency	1520:1529	arg1	tests					1531:1535	The cation dependency tests	1509:1535	The cation dependency tests	1509:1535	The cation dependency tests revealed that the monovalent and divalent cations are highly suitable for flocculation while the trivalent cations showed moderate flocculation.
35567323	1	76	theme	bacterial	160:168	arg1	strain					170:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain	132:175	The bioflocculant producing bacterial strain - UKD24 was isolated from the domestic sewage treatment plant.
37193733	4	0	theme	engineered	579:588	arg1	Fbs1					620:623	Fbs1	620:623	Fbs1	620:623	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	4	0	theme	engineered	579:588	arg1	protein					611:617	an engineered carbohydrate-binding protein	576:617	an engineered carbohydrate-binding protein	576:617	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	6	1	theme	Fbs1	814:817	arg1	enrichment					819:828	Fbs1 enrichment	814:828	Fbs1 enrichment of N-glycosylated peptides	814:855	Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites.
37193733	8	2	theme	localization	996:1007	arg1	prediction					1009:1018	Gene ontology and cell localization prediction	973:1018	Gene ontology and cell localization prediction of the identified N-glycoproteins	973:1052	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	2	3	theme	host	290:293	arg1	interactions					304:315	host parasite interactions	290:315	host parasite interactions	290:315	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	2	4	located	present	220:226	arg1	surface					231:237	surface	231:237	surface	231:237	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	2	4	located	present	220:226	arg2	glycans					208:214	N-linked glycans	199:214	N-linked glycans	199:214	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	2	4	located	present	220:226	arg1	proteins					261:268	secreted filarial proteins	243:268	secreted filarial proteins	243:268	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	4	5	gly	N-glycosylated	636:649	arg1	peptides					651:658	N-glycosylated peptides	636:658	N-glycosylated peptides	636:658	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	1	6	theme	proteins	189:196	arg1	glycosylation					115:127	N-linked glycosylation	106:127	N-linked glycosylation	106:127	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	1	6	theme	proteins	189:196	arg1	modification					162:173	a critical post translational modification	132:173	a critical post translational modification of eukaryotic proteins	132:196	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	6	7	theme	N-glycosites	888:899	arg1	identification					870:883	the identification	866:883	the identification of N-glycosites	866:899	Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites.
37193733	3	8	link	N-linked	447:454	arg1	glycoproteome					456:468	the N-linked glycoproteome	443:468	the N-linked glycoproteome of this or any other filarial parasite	443:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	8	9	theme	identified	1027:1036	arg1	N-glycoproteins					1038:1052	the identified N-glycoproteins	1023:1052	the identified N-glycoproteins	1023:1052	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	9	10	from	microfilariae	1188:1200	arg1	results					1133:1139	results	1133:1139	results from adult female worms, adult male worms, and microfilariae	1133:1200	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	10	11	gly	N-glycoproteins	1355:1369	arg1	N-glycoproteins					1355:1369	cuticle N-glycoproteins	1347:1369	cuticle N-glycoproteins	1347:1369	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	10	12	theme	parasite	1449:1456	arg1	interface					1458:1466	the host parasite interface	1440:1466	the host parasite interface	1440:1466	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	9	13	gly	N-glycosite	1292:1302	arg2	N-glycosite					1292:1302	the individual N-glycosite level	1277:1308	the individual N-glycosite level	1277:1308	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	5	14	from	stages	746:751	arg1	proteins					721:728	proteins	721:728	proteins from three host stages of the parasite: adult female, adult male and microfilariae	721:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	8	15	gly	N-glycoproteins	1038:1052	arg1	N-glycoproteins					1038:1052	the identified N-glycoproteins	1023:1052	the identified N-glycoproteins	1023:1052	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	2	16	theme	secreted	243:250	arg1	proteins					261:268	secreted filarial proteins	243:268	secreted filarial proteins	243:268	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	5	17	dep	stages	746:751	arg1	stages					746:751	three host stages	735:751	three host stages of the parasite: adult female, adult male and microfilariae	735:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	17	dep	stages	746:751	arg1	microfilariae					799:811	microfilariae	799:811	microfilariae	799:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	17	dep	stages	746:751	arg1	female					776:781	adult female	770:781	adult female	770:781	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	17	dep	stages	746:751	arg1	male					790:793	adult male	784:793	adult male	784:793	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	9	18	theme	individual	1281:1290	arg1	level					1304:1308	the individual N-glycosite level	1277:1308	the individual N-glycosite level	1277:1308	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	1	19	theme	critical	134:141	arg1	glycosylation					115:127	N-linked glycosylation	106:127	N-linked glycosylation	106:127	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	1	19	theme	critical	134:141	arg1	modification					162:173	a critical post translational modification	132:173	a critical post translational modification of eukaryotic proteins	132:196	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	3	20	theme	malayi	350:355	arg1	proteins					357:364	glycosylated Brugia malayi proteins	330:364	glycosylated Brugia malayi proteins	330:364	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	5	21	theme	parasite	760:767	arg1	stages					746:751	three host stages	735:751	three host stages of the parasite: adult female, adult male and microfilariae	735:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	21	theme	parasite	760:767	arg1	microfilariae					799:811	microfilariae	799:811	microfilariae	799:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	21	theme	parasite	760:767	arg1	female					776:781	adult female	770:781	adult female	770:781	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	21	theme	parasite	760:767	arg1	male					790:793	adult male	784:793	adult male	784:793	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	1	22	theme	translational	148:160	arg1	glycosylation					115:127	N-linked glycosylation	106:127	N-linked glycosylation	106:127	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	1	22	theme	translational	148:160	arg1	modification					162:173	a critical post translational modification	132:173	a critical post translational modification of eukaryotic proteins	132:196	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	3	23	theme	glycosylated	330:341	arg1	proteins					357:364	glycosylated Brugia malayi proteins	330:364	glycosylated Brugia malayi proteins	330:364	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	10	24	theme	therapeutic	1506:1516	arg1	targets					1518:1524	potential therapeutic targets	1496:1524	potential therapeutic targets	1496:1524	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	9	25	from	variability	1211:1221	arg1	N-glycosylation					1226:1240	N-glycosylation	1226:1240	N-glycosylation	1226:1240	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	0	26	theme	parasitic	72:80	arg1	malayi					98:103	the human parasitic nematode Brugia malayi	62:103	the human parasitic nematode Brugia malayi	62:103	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	0	27	theme	Brugia	91:96	arg1	malayi					98:103	the human parasitic nematode Brugia malayi	62:103	the human parasitic nematode Brugia malayi	62:103	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	3	28	theme	N-linked	447:454	arg1	glycoproteome					456:468	the N-linked glycoproteome	443:468	the N-linked glycoproteome of this or any other filarial parasite	443:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	9	29	theme	adult	1146:1150	arg1	worms					1159:1163	adult female worms	1146:1163	adult female worms	1146:1163	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	2	30	link	N-linked	199:206	arg1	glycans					208:214	N-linked glycans	199:214	N-linked glycans	199:214	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	4	31	theme	N-glyco	548:554	arg1	protocol					561:568	an enhanced N-glyco FASP protocol	536:568	an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1,	536:624	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	0	32	theme	filarial	13:20	arg1	N-glycoproteome					22:36	the filarial N-glycoproteome	9:36	the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi	9:103	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	1	33	theme	N-linked	106:113	arg1	glycosylation					115:127	N-linked glycosylation	106:127	N-linked glycosylation	106:127	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	1	33	theme	N-linked	106:113	arg1	modification					162:173	a critical post translational modification	132:173	a critical post translational modification of eukaryotic proteins	132:196	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	3	34	theme	systematic	423:432	arg1	study					434:438	a systematic study	421:438	a systematic study of the N-linked glycoproteome of this or any other filarial parasite	421:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	9	35	theme	adult	1166:1170	arg1	worms					1177:1181	adult male worms	1166:1181	adult male worms	1166:1181	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	10	36	theme	restricted	1386:1395	arg1	N-glycoproteins					1397:1411	adult worm restricted N-glycoproteins	1375:1411	adult worm restricted N-glycoproteins	1375:1411	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	6	37	theme	peptides	848:855	arg1	enrichment					819:828	Fbs1 enrichment	814:828	Fbs1 enrichment of N-glycosylated peptides	814:855	Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites.
37193733	10	38	theme	adult	1375:1379	arg1	N-glycoproteins					1397:1411	adult worm restricted N-glycoproteins	1375:1411	adult worm restricted N-glycoproteins	1375:1411	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	3	39	theme	other	485:489	arg1	glycoproteome					456:468	the N-linked glycoproteome	443:468	the N-linked glycoproteome of this or any other filarial parasite	443:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	3	40	gly	glycosylated	330:341	arg1	proteins					357:364	glycosylated Brugia malayi proteins	330:364	glycosylated Brugia malayi proteins	330:364	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	0	41	gly	glycosite	41:49	arg2	glycosite					41:49	glycosite mapping	41:57	glycosite mapping	41:57	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	8	42	theme	ontology	978:985	arg1	prediction					1009:1018	Gene ontology and cell localization prediction	973:1018	Gene ontology and cell localization prediction of the identified N-glycoproteins	973:1052	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	7	43	gly	N-glycosites	959:970	arg2	N-glycosites					959:970	1273 N-glycosites	954:970	1273 N-glycosites	954:970	Our data identified 582 N-linked glycoproteins with 1273 N-glycosites.
37193733	8	44	theme	cell	991:994	arg1	localization					996:1007	cell localization	991:1007	cell localization	991:1007	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	10	45	from	interface	1458:1466	arg1	examples					1416:1423	examples	1416:1423	examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers	1416:1538	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	10	45	from	interface	1458:1466	arg1	variations					1317:1326	These variations	1311:1326	These variations	1311:1326	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	10	45	from	interface	1458:1466	arg1	proteins					1428:1435	proteins	1428:1435	proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers	1428:1538	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	7	46	with	glycoproteins	935:947	arg1	N-glycosites					959:970	1273 N-glycosites	954:970	1273 N-glycosites	954:970	Our data identified 582 N-linked glycoproteins with 1273 N-glycosites.
37193733	10	47	theme	host	1444:1447	arg1	interface					1458:1466	the host parasite interface	1440:1466	the host parasite interface	1440:1466	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	2	48	theme	parasite	295:302	arg1	interactions					304:315	host parasite interactions	290:315	host parasite interactions	290:315	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	5	49	from	N-glycosites	705:716	arg1	proteins					721:728	proteins	721:728	proteins from three host stages of the parasite: adult female, adult male and microfilariae	721:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	4	50	theme	carbohydrate-binding	590:609	arg1	Fbs1					620:623	Fbs1	620:623	Fbs1	620:623	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	4	50	theme	carbohydrate-binding	590:609	arg1	protein					611:617	an engineered carbohydrate-binding protein	576:617	an engineered carbohydrate-binding protein	576:617	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	1	51	theme	eukaryotic	178:187	arg1	proteins					189:196	eukaryotic proteins	178:196	eukaryotic proteins	178:196	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	8	52	theme	N-glycoproteins	1038:1052	arg1	prediction					1009:1018	Gene ontology and cell localization prediction	973:1018	Gene ontology and cell localization prediction of the identified N-glycoproteins	973:1052	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	5	53	theme	adult	770:774	arg1	stages					746:751	three host stages	735:751	three host stages of the parasite: adult female, adult male and microfilariae	735:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	53	theme	adult	770:774	arg1	female					776:781	adult female	770:781	adult female	770:781	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	9	54	theme	protein	1249:1255	arg1	level					1257:1261	the protein level	1245:1261	the protein level	1245:1261	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	2	55	theme	filarial	252:259	arg1	proteins					261:268	secreted filarial proteins	243:268	secreted filarial proteins	243:268	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	9	56	from	worms	1177:1181	arg1	results					1133:1139	results	1133:1139	results from adult female worms, adult male worms, and microfilariae	1133:1200	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	7	57	gly	glycoproteins	935:947	arg1	glycoproteins					935:947	582 N-linked glycoproteins	922:947	582 N-linked glycoproteins with 1273 N-glycosites	922:970	Our data identified 582 N-linked glycoproteins with 1273 N-glycosites.
37193733	5	58	gly	N-glycosites	705:716	arg2	N-glycosites					705:716	the N-glycosites	701:716	the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae	701:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	9	59	theme	N-glycosite	1292:1302	arg1	level					1304:1308	the individual N-glycosite level	1277:1308	the individual N-glycosite level	1277:1308	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	6	60	gly	N-glycosites	888:899	arg2	N-glycosites					888:899	N-glycosites	888:899	N-glycosites	888:899	Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites.
37193733	0	61	theme	human	66:70	arg1	malayi					98:103	the human parasitic nematode Brugia malayi	62:103	the human parasitic nematode Brugia malayi	62:103	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	4	62	theme	N-glycosylated	636:649	arg1	peptides					651:658	N-glycosylated peptides	636:658	N-glycosylated peptides	636:658	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	1	63	theme	post	143:146	arg1	glycosylation					115:127	N-linked glycosylation	106:127	N-linked glycosylation	106:127	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	1	63	theme	post	143:146	arg1	modification					162:173	a critical post translational modification	132:173	a critical post translational modification of eukaryotic proteins	132:196	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	3	64	theme	proteins	357:364	arg1	Examples					318:325	Examples	318:325	Examples of glycosylated Brugia malayi proteins	318:364	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	0	65	from	N-glycoproteome	22:36	arg1	malayi					98:103	the human parasitic nematode Brugia malayi	62:103	the human parasitic nematode Brugia malayi	62:103	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	5	66	theme	host	741:744	arg1	stages					746:751	three host stages	735:751	three host stages of the parasite: adult female, adult male and microfilariae	735:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	66	theme	host	741:744	arg1	microfilariae					799:811	microfilariae	799:811	microfilariae	799:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	66	theme	host	741:744	arg1	female					776:781	adult female	770:781	adult female	770:781	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	66	theme	host	741:744	arg1	male					790:793	adult male	784:793	adult male	784:793	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	0	67	theme	nematode	82:89	arg1	malayi					98:103	the human parasitic nematode Brugia malayi	62:103	the human parasitic nematode Brugia malayi	62:103	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	3	68	theme	Brugia	343:348	arg1	malayi					350:355	Brugia malayi	343:355	glycosylated Brugia malayi proteins	330:364	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	10	69	theme	potential	1496:1504	arg1	targets					1518:1524	potential therapeutic targets	1496:1524	potential therapeutic targets	1496:1524	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	3	70	dep	other	485:489	arg1	parasite					500:507	filarial parasite	491:507	any other filarial parasite	481:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	10	71	gly	N-glycoproteins	1397:1411	arg1	N-glycoproteins					1397:1411	adult worm restricted N-glycoproteins	1375:1411	adult worm restricted N-glycoproteins	1375:1411	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	6	72	gly	N-glycosylated	833:846	arg1	peptides					848:855	N-glycosylated peptides	833:855	N-glycosylated peptides	833:855	Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites.
37193733	8	73	theme	extracellular	1099:1111	arg1	proteins					1113:1120	extracellular proteins	1099:1120	extracellular proteins	1099:1120	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	2	74	attach	present	220:226	arg1	surface					231:237	surface	231:237	surface	231:237	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	2	74	attach	present	220:226	arg2	glycans					208:214	N-linked glycans	199:214	N-linked glycans	199:214	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	2	74	attach	present	220:226	arg1	proteins					261:268	secreted filarial proteins	243:268	secreted filarial proteins	243:268	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	3	75	theme	glycoproteome	456:468	arg1	study					434:438	a systematic study	421:438	a systematic study of the N-linked glycoproteome of this or any other filarial parasite	421:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	4	76	theme	FASP	556:559	arg1	protocol					561:568	an enhanced N-glyco FASP protocol	536:568	an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1,	536:624	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	9	77	theme	female	1152:1157	arg1	worms					1159:1163	adult female worms	1146:1163	adult female worms	1146:1163	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	10	78	from	examples	1416:1423	arg1	interface					1458:1466	the host parasite interface	1440:1466	the host parasite interface	1440:1466	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	2	79	theme	N-linked	199:206	arg1	glycans					208:214	N-linked glycans	199:214	N-linked glycans	199:214	N-linked glycans are present on surface and secreted filarial proteins that play a role in host parasite interactions.
37193733	10	80	theme	cuticle	1347:1353	arg1	N-glycoproteins					1355:1369	cuticle N-glycoproteins	1347:1369	cuticle N-glycoproteins	1347:1369	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	0	81	theme	glycosite	41:49	arg1	mapping					51:57	glycosite mapping	41:57	glycosite mapping	41:57	Defining the filarial N-glycoproteome by glycosite mapping in the human parasitic nematode Brugia malayi.
37193733	9	82	theme	male	1172:1175	arg1	worms					1177:1181	adult male worms	1166:1181	adult male worms	1166:1181	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	3	83	theme	this	473:476	arg1	glycoproteome					456:468	the N-linked glycoproteome	443:468	the N-linked glycoproteome of this or any other filarial parasite	443:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	5	84	theme	adult	784:788	arg1	stages					746:751	three host stages	735:751	three host stages of the parasite: adult female, adult male and microfilariae	735:811	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	5	84	theme	adult	784:788	arg1	male					790:793	adult male	784:793	adult male	784:793	We then mapped the N-glycosites on proteins from three host stages of the parasite: adult female, adult male and microfilariae.
37193733	4	85	theme	enhanced	539:546	arg1	protocol					561:568	an enhanced N-glyco FASP protocol	536:568	an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1,	536:624	In this study, we applied an enhanced N-glyco FASP protocol using an engineered carbohydrate-binding protein, Fbs1, to enrich N-glycosylated peptides for analysis by LC-MS/MS.
37193733	9	86	from	worms	1159:1163	arg1	results					1133:1139	results	1133:1139	results from adult female worms, adult male worms, and microfilariae	1133:1200	Comparing results from adult female worms, adult male worms, and microfilariae, we find variability in N-glycosylation at the protein level as well as at the individual N-glycosite level.
37193733	10	87	theme	worm	1381:1384	arg1	N-glycoproteins					1397:1411	adult worm restricted N-glycoproteins	1375:1411	adult worm restricted N-glycoproteins	1375:1411	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	7	88	theme	N-linked	926:933	arg1	glycoproteins					935:947	582 N-linked glycoproteins	922:947	582 N-linked glycoproteins with 1273 N-glycosites	922:970	Our data identified 582 N-linked glycoproteins with 1273 N-glycosites.
37193733	6	89	theme	N-glycosylated	833:846	arg1	peptides					848:855	N-glycosylated peptides	833:855	N-glycosylated peptides	833:855	Fbs1 enrichment of N-glycosylated peptides enhanced the identification of N-glycosites.
37193733	7	90	link	N-linked	926:933	arg1	glycoproteins					935:947	582 N-linked glycoproteins	922:947	582 N-linked glycoproteins with 1273 N-glycosites	922:970	Our data identified 582 N-linked glycoproteins with 1273 N-glycosites.
37193733	10	91	theme	proteins	1428:1435	arg1	examples					1416:1423	examples	1416:1423	examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers	1416:1538	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	10	91	theme	proteins	1428:1435	arg1	variations					1317:1326	These variations	1311:1326	These variations	1311:1326	These variations are highlighted in cuticle N-glycoproteins and adult worm restricted N-glycoproteins as examples of proteins at the host parasite interface that are well positioned as potential therapeutic targets or biomarkers.
37193733	3	92	theme	filarial	491:498	arg1	parasite					500:507	filarial parasite	491:507	any other filarial parasite	481:507	Examples of glycosylated Brugia malayi proteins have been previously identified but there has not been a systematic study of the N-linked glycoproteome of this or any other filarial parasite.
37193733	8	93	theme	Gene	973:976	arg1	ontology					978:985	Gene ontology	973:985	Gene ontology	973:985	Gene ontology and cell localization prediction of the identified N-glycoproteins indicated that they were mostly membrane and extracellular proteins.
37193733	1	94	link	N-linked	106:113	arg1	glycosylation					115:127	N-linked glycosylation	106:127	N-linked glycosylation	106:127	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
37193733	1	94	link	N-linked	106:113	arg1	modification					162:173	a critical post translational modification	132:173	a critical post translational modification of eukaryotic proteins	132:196	N-linked glycosylation is a critical post translational modification of eukaryotic proteins.
36429327	4	0	theme	1.19	817:820	arg1	g/g					822:824	13.43 ± 1.19 g/g	809:824	13.43 ± 1.19 g/g	809:824	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	0	theme	1.19	817:820	arg1	capacity					799:806	water retention capacity	783:806	water retention capacity (13.43 ± 1.19 g/g)	783:825	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	2	1	theme	microwave	333:341	arg1	method					357:362	the combined microwave and enzymatic method	320:362	the combined microwave and enzymatic method to obtain SDF	320:376	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	9	2	contain	had	1426:1428	arg1	GP-IDF-SDF					1415:1424	GP-IDF-SDF	1415:1424	GP-IDF-SDF	1415:1424	Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.
36429327	9	2	contain	had	1426:1428	arg2	characteristics					1441:1455	functional characteristics	1430:1455	functional characteristics	1430:1455	Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.
36429327	2	3	theme	dietary	279:285	arg1	GP-IDF					294:299	GP-IDF	294:299	GP-IDF	294:299	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	2	3	theme	dietary	279:285	arg1	fiber					287:291	grapefruit peel insoluble dietary fiber	253:291	grapefruit peel insoluble dietary fiber (GP-IDF)	253:300	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	3	4	theme	monosaccharide	652:665	arg1	compositions					667:678	the main monosaccharide compositions	643:678	the main monosaccharide compositions	643:678	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	4	theme	monosaccharide	652:665	arg1	acid					633:636	glucuronic acid	622:636	glucuronic acid	622:636	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	4	theme	monosaccharide	652:665	arg1	acid					602:605	200 °C. Galacturonic acid	581:605	200 °C. Galacturonic acid	581:605	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	4	theme	monosaccharide	652:665	arg1	arabinose					608:616	arabinose	608:616	arabinose	608:616	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	2	5	theme	peel	264:267	arg1	GP-IDF					294:299	GP-IDF	294:299	GP-IDF	294:299	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	2	5	theme	peel	264:267	arg1	fiber					287:291	grapefruit peel insoluble dietary fiber	253:291	grapefruit peel insoluble dietary fiber (GP-IDF)	253:300	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	7	6	theme	jam	1269:1271	arg1	stability					1256:1264	the stability	1252:1264	the stability of jam	1252:1271	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	3	7	theme	type	536:539	arg1	crystal					543:549	a typical cellulose type I crystal	516:549	a typical cellulose type I crystal	516:549	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	4	8	theme	22.10	852:856	arg1	g/g					865:867	22.10 ± 0.85 g/g	852:867	22.10 ± 0.85 g/g	852:867	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	8	theme	22.10	852:856	arg1	capacity					842:849	oil retention capacity	828:849	oil retention capacity (22.10 ± 0.85 g/g)	828:868	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	5	9	theme	commercial	966:975	arg1	addition					984:991	commercial pectin addition	966:991	commercial pectin addition	966:991	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	8	10	with	texture	1312:1318	arg1	GP-IDF-SDF					1350:1359	GP-IDF-SDF	1350:1359	GP-IDF-SDF	1350:1359	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	8	10	with	texture	1312:1318	arg1	pectin					1364:1369	pectin	1364:1369	pectin	1364:1369	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	1	11	theme	dietary	217:223	arg1	SDF					232:234	SDF	232:234	SDF	232:234	Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF).
36429327	1	11	theme	dietary	217:223	arg1	fiber					225:229	soluble dietary fiber	209:229	soluble dietary fiber (SDF)	209:235	Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF).
36429327	4	12	theme	0.85	860:863	arg1	g/g					865:867	22.10 ± 0.85 g/g	852:867	22.10 ± 0.85 g/g	852:867	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	12	theme	0.85	860:863	arg1	capacity					842:849	oil retention capacity	828:849	oil retention capacity (22.10 ± 0.85 g/g)	828:868	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	3	13	theme	principal	724:732	arg1	pectin					697:702	pectin	697:702	pectin	697:702	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	13	theme	principal	724:732	arg1	component					734:742	the principal component	720:742	the principal component	720:742	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	5	14	theme	addition	984:991	arg1	effects					940:946	the effects	936:946	the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam	936:1063	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	4	15	theme	oil	828:830	arg1	g/g					865:867	22.10 ± 0.85 g/g	852:867	22.10 ± 0.85 g/g	852:867	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	15	theme	oil	828:830	arg1	capacity					842:849	oil retention capacity	828:849	oil retention capacity (22.10 ± 0.85 g/g)	828:868	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	16	from	capacity	842:849	arg1	excellent					770:778	excellent	770:778	excellent	770:778	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	17	from	excellent	770:778	arg1	mg/g					917:920	14.49 ± 0.068 mg/g	903:920	14.49 ± 0.068 mg/g	903:920	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	17	from	excellent	770:778	arg1	capacity					799:806	water retention capacity	783:806	water retention capacity (13.43 ± 1.19 g/g)	783:825	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	17	from	excellent	770:778	arg1	capacity					893:900	glucose adsorption capacity	874:900	glucose adsorption capacity (14.49 ± 0.068 mg/g)	874:921	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	17	from	excellent	770:778	arg1	g/g					865:867	22.10 ± 0.85 g/g	852:867	22.10 ± 0.85 g/g	852:867	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	17	from	excellent	770:778	arg1	capacity					842:849	oil retention capacity	828:849	oil retention capacity (22.10 ± 0.85 g/g)	828:868	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	17	from	excellent	770:778	arg1	g/g					822:824	13.43 ± 1.19 g/g	809:824	13.43 ± 1.19 g/g	809:824	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	9	18	theme	food	1563:1566	arg1	ingredients					1568:1578	functional food ingredients	1552:1578	functional food ingredients	1552:1578	Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.
36429327	7	19	theme	jam	1231:1233	arg1	viscosity					1201:1209	viscosity	1201:1209	viscosity	1201:1209	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	7	19	theme	jam	1231:1233	arg1	strength					1219:1226	gel strength	1215:1226	gel strength	1215:1226	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	5	20	theme	GP-IDF-SDF	951:960	arg1	effects					940:946	the effects	936:946	the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam	936:1063	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	6	21	with	color	1108:1112	arg1	GP-IDF-SDF					1126:1135	GP-IDF-SDF	1126:1135	GP-IDF-SDF	1126:1135	The results showed that the color of jam with GP-IDF-SDF was lighter.
36429327	0	22	from	Fiber	105:109	arg1	Jam					140:142	Blueberry Jam	130:142	Blueberry Jam	130:142	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	0	23	theme	Grapefruit	71:80	arg1	Fiber					105:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	3	24	theme	good	560:563	arg1	sheet					485:489	a flat sheet	478:489	a flat sheet	478:489	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	24	theme	good	560:563	arg1	stability					565:573	good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component	560:742	good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component	560:742	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	1	25	theme	modification	157:168	arg1	methods					170:176	Appropriate modification methods	145:176	Appropriate modification methods	145:176	Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF).
36429327	8	26	theme	jam	1341:1343	arg1	color					1305:1309	color	1305:1309	color	1305:1309	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	8	26	theme	jam	1341:1343	arg1	spreadability					1324:1336	spreadability	1324:1336	spreadability	1324:1336	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	8	26	theme	jam	1341:1343	arg1	texture					1312:1318	texture	1312:1318	texture	1312:1318	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	4	27	theme	retention	832:840	arg1	g/g					865:867	22.10 ± 0.85 g/g	852:867	22.10 ± 0.85 g/g	852:867	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	27	theme	retention	832:840	arg1	capacity					842:849	oil retention capacity	828:849	oil retention capacity (22.10 ± 0.85 g/g)	828:868	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	0	28	theme	Insoluble	87:95	arg1	Fiber					105:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	4	29	theme	glucose	874:880	arg1	mg/g					917:920	14.49 ± 0.068 mg/g	903:920	14.49 ± 0.068 mg/g	903:920	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	29	theme	glucose	874:880	arg1	capacity					893:900	glucose adsorption capacity	874:900	glucose adsorption capacity (14.49 ± 0.068 mg/g)	874:921	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	30	from	capacity	799:806	arg1	excellent					770:778	excellent	770:778	excellent	770:778	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	7	31	theme	gel	1215:1217	arg1	strength					1219:1226	gel strength	1215:1226	gel strength	1215:1226	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	0	32	theme	Physicochemical	0:14	arg1	Characteristics					16:30	Physicochemical Characteristics	0:30	Physicochemical Characteristics of Soluble Dietary Fiber	0:55	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	9	33	theme	functional	1430:1439	arg1	characteristics					1441:1455	functional characteristics	1430:1455	functional characteristics	1430:1455	Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.
36429327	3	34	theme	grapefruit	432:441	arg1	GP-IDF-SDF					453:462	GP-IDF-SDF	453:462	GP-IDF-SDF	453:462	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	34	theme	grapefruit	432:441	arg1	IDF					448:450	grapefruit peel IDF	432:450	grapefruit peel IDF (GP-IDF-SDF)	432:463	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	0	35	theme	Dietary	43:49	arg1	Fiber					51:55	Soluble Dietary Fiber	35:55	Soluble Dietary Fiber	35:55	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	7	36	dep	viscosity	1201:1209	arg1	the					1197:1199	the	1197:1199	the	1197:1199	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	7	37	theme	less	1181:1184	arg1	effects					1186:1192	less effects	1181:1192	less effects	1181:1192	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	3	38	theme	°C.	585:587	arg1	compositions					667:678	the main monosaccharide compositions	643:678	the main monosaccharide compositions	643:678	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	38	theme	°C.	585:587	arg1	acid					633:636	glucuronic acid	622:636	glucuronic acid	622:636	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	38	theme	°C.	585:587	arg1	acid					602:605	200 °C. Galacturonic acid	581:605	200 °C. Galacturonic acid	581:605	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	38	theme	°C.	585:587	arg1	arabinose					608:616	arabinose	608:616	arabinose	608:616	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	4	39	theme	water	783:787	arg1	g/g					822:824	13.43 ± 1.19 g/g	809:824	13.43 ± 1.19 g/g	809:824	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	39	theme	water	783:787	arg1	capacity					799:806	water retention capacity	783:806	water retention capacity (13.43 ± 1.19 g/g)	783:825	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	2	40	theme	combined	324:331	arg1	method					357:362	the combined microwave and enzymatic method	320:362	the combined microwave and enzymatic method to obtain SDF	320:376	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	5	41	theme	blueberry	1051:1059	arg1	jam					1061:1063	blueberry jam	1051:1063	blueberry jam	1051:1063	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	7	42	theme	GP-IDF-SDF	1166:1175	arg1	addition					1154:1161	The addition	1150:1161	The addition of GP-IDF-SDF	1150:1175	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	5	43	dep	color	1000:1004	arg1	the					996:998	the	996:998	the	996:998	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	3	44	theme	glucuronic	622:631	arg1	compositions					667:678	the main monosaccharide compositions	643:678	the main monosaccharide compositions	643:678	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	44	theme	glucuronic	622:631	arg1	acid					633:636	glucuronic acid	622:636	glucuronic acid	622:636	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	44	theme	glucuronic	622:631	arg1	acid					602:605	200 °C. Galacturonic acid	581:605	200 °C. Galacturonic acid	581:605	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	44	theme	glucuronic	622:631	arg1	arabinose					608:616	arabinose	608:616	arabinose	608:616	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	45	contain	had	556:558	arg1	SDF					423:425	SDF	423:425	SDF from grapefruit peel IDF (GP-IDF-SDF)	423:463	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	45	contain	had	556:558	arg2	stability					565:573	good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component	560:742	good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component	560:742	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	45	contain	had	556:558	arg2	sheet					485:489	a flat sheet	478:489	a flat sheet	478:489	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	4	46	from	capacity	893:900	arg1	excellent					770:778	excellent	770:778	excellent	770:778	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	3	47	theme	main	647:650	arg1	compositions					667:678	the main monosaccharide compositions	643:678	the main monosaccharide compositions	643:678	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	47	theme	main	647:650	arg1	acid					633:636	glucuronic acid	622:636	glucuronic acid	622:636	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	47	theme	main	647:650	arg1	acid					602:605	200 °C. Galacturonic acid	581:605	200 °C. Galacturonic acid	581:605	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	47	theme	main	647:650	arg1	arabinose					608:616	arabinose	608:616	arabinose	608:616	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	4	48	theme	±	815:815	arg1	g/g					822:824	13.43 ± 1.19 g/g	809:824	13.43 ± 1.19 g/g	809:824	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	48	theme	±	815:815	arg1	capacity					799:806	water retention capacity	783:806	water retention capacity (13.43 ± 1.19 g/g)	783:825	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	2	49	theme	insoluble	269:277	arg1	GP-IDF					294:299	GP-IDF	294:299	GP-IDF	294:299	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	2	49	theme	insoluble	269:277	arg1	fiber					287:291	grapefruit peel insoluble dietary fiber	253:291	grapefruit peel insoluble dietary fiber (GP-IDF)	253:300	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	5	50	from	effects	940:946	arg1	texture					1017:1023	texture	1017:1023	texture	1017:1023	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	5	50	from	effects	940:946	arg1	properties					1037:1046	sensory properties	1029:1046	sensory properties	1029:1046	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	5	50	from	effects	940:946	arg1	rheology					1007:1014	rheology	1007:1014	rheology	1007:1014	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	5	50	from	effects	940:946	arg1	color					1000:1004	color	1000:1004	color	1000:1004	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	4	51	theme	retention	789:797	arg1	g/g					822:824	13.43 ± 1.19 g/g	809:824	13.43 ± 1.19 g/g	809:824	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	51	theme	retention	789:797	arg1	capacity					799:806	water retention capacity	783:806	water retention capacity (13.43 ± 1.19 g/g)	783:825	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	3	52	theme	cellulose	526:534	arg1	crystal					543:549	a typical cellulose type I crystal	516:549	a typical cellulose type I crystal	516:549	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	0	53	theme	Blueberry	130:138	arg1	Jam					140:142	Blueberry Jam	130:142	Blueberry Jam	130:142	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	1	54	theme	soluble	209:215	arg1	SDF					232:234	SDF	232:234	SDF	232:234	Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF).
36429327	1	54	theme	soluble	209:215	arg1	fiber					225:229	soluble dietary fiber	209:229	soluble dietary fiber (SDF)	209:235	Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF).
36429327	3	55	from	IDF	448:450	arg1	SDF					423:425	SDF	423:425	SDF from grapefruit peel IDF (GP-IDF-SDF)	423:463	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	7	56	contain	had	1177:1179	arg1	addition					1154:1161	The addition	1150:1161	The addition of GP-IDF-SDF	1150:1175	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	7	56	contain	had	1177:1179	arg2	effects					1186:1192	less effects	1181:1192	less effects	1181:1192	The addition of GP-IDF-SDF had less effects on the viscosity and gel strength of jam, but it enhanced the stability of jam.
36429327	1	57	theme	fiber	225:229	arg1	proportion					195:204	the proportion	191:204	the proportion of soluble dietary fiber (SDF)	191:235	Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF).
36429327	4	58	theme	±	858:858	arg1	g/g					865:867	22.10 ± 0.85 g/g	852:867	22.10 ± 0.85 g/g	852:867	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	58	theme	±	858:858	arg1	capacity					842:849	oil retention capacity	828:849	oil retention capacity (22.10 ± 0.85 g/g)	828:868	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	6	59	theme	jam	1117:1119	arg1	lighter					1141:1147	lighter	1141:1147	lighter	1141:1147	The results showed that the color of jam with GP-IDF-SDF was lighter.
36429327	6	59	theme	jam	1117:1119	arg1	color					1108:1112	the color	1104:1112	the color of jam with GP-IDF-SDF	1104:1135	The results showed that the color of jam with GP-IDF-SDF was lighter.
36429327	3	60	theme	I	541:541	arg1	crystal					543:549	a typical cellulose type I crystal	516:549	a typical cellulose type I crystal	516:549	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	9	61	theme	functional	1552:1561	arg1	ingredients					1568:1578	functional food ingredients	1552:1578	functional food ingredients	1552:1578	Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.
36429327	0	62	from	Effects	119:125	arg1	Jam					140:142	Blueberry Jam	130:142	Blueberry Jam	130:142	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	2	63	theme	grapefruit	253:262	arg1	GP-IDF					294:299	GP-IDF	294:299	GP-IDF	294:299	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	2	63	theme	grapefruit	253:262	arg1	fiber					287:291	grapefruit peel insoluble dietary fiber	253:291	grapefruit peel insoluble dietary fiber (GP-IDF)	253:300	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	9	64	theme	ingredients	1568:1578	arg1	development					1537:1547	the development	1533:1547	the development of functional food ingredients	1533:1578	Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.
36429327	0	65	theme	Peel	82:85	arg1	Fiber					105:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	1	66	theme	Appropriate	145:155	arg1	methods					170:176	Appropriate modification methods	145:176	Appropriate modification methods	145:176	Appropriate modification methods can increase the proportion of soluble dietary fiber (SDF).
36429327	4	67	theme	adsorption	882:891	arg1	mg/g					917:920	14.49 ± 0.068 mg/g	903:920	14.49 ± 0.068 mg/g	903:920	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	4	67	theme	adsorption	882:891	arg1	capacity					893:900	glucose adsorption capacity	874:900	glucose adsorption capacity (14.49 ± 0.068 mg/g)	874:921	Moreover, GP-IDF-SDF was excellent in water retention capacity (13.43 ± 1.19 g/g), oil retention capacity (22.10 ± 0.85 g/g) and glucose adsorption capacity (14.49 ± 0.068 mg/g).
36429327	0	68	theme	Dietary	97:103	arg1	Fiber					105:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Grapefruit Peel Insoluble Dietary Fiber	71:109	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	5	69	theme	sensory	1029:1035	arg1	properties					1037:1046	sensory properties	1029:1046	sensory properties	1029:1046	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	3	70	theme	typical	518:524	arg1	crystal					543:549	a typical cellulose type I crystal	516:549	a typical cellulose type I crystal	516:549	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	5	71	theme	pectin	977:982	arg1	addition					984:991	commercial pectin addition	966:991	commercial pectin addition	966:991	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	0	72	theme	Soluble	35:41	arg1	Fiber					51:55	Soluble Dietary Fiber	35:55	Soluble Dietary Fiber	35:55	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	3	73	theme	Galacturonic	589:600	arg1	compositions					667:678	the main monosaccharide compositions	643:678	the main monosaccharide compositions	643:678	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	73	theme	Galacturonic	589:600	arg1	acid					633:636	glucuronic acid	622:636	glucuronic acid	622:636	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	73	theme	Galacturonic	589:600	arg1	acid					602:605	200 °C. Galacturonic acid	581:605	200 °C. Galacturonic acid	581:605	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	73	theme	Galacturonic	589:600	arg1	arabinose					608:616	arabinose	608:616	arabinose	608:616	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	0	74	theme	Fiber	51:55	arg1	Characteristics					16:30	Physicochemical Characteristics	0:30	Physicochemical Characteristics of Soluble Dietary Fiber	0:55	Physicochemical Characteristics of Soluble Dietary Fiber Obtained from Grapefruit Peel Insoluble Dietary Fiber and Its Effects on Blueberry Jam.
36429327	3	75	dep	characterization	405:420	arg1	regard					384:389	regard	384:389	regard	384:389	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	76	theme	structural	394:403	arg1	characterization					405:420	structural characterization	394:420	structural characterization	394:420	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	77	theme	200	581:583	arg1	°C.					585:587	°C.	585:587	°C.	585:587	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	78	theme	peel	443:446	arg1	GP-IDF-SDF					453:462	GP-IDF-SDF	453:462	GP-IDF-SDF	453:462	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	78	theme	peel	443:446	arg1	IDF					448:450	grapefruit peel IDF	432:450	grapefruit peel IDF (GP-IDF-SDF)	432:463	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	8	79	dep	color	1305:1309	arg1	the					1301:1303	the	1301:1303	the	1301:1303	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	3	80	theme	flat	480:483	arg1	stability					565:573	good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component	560:742	good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component	560:742	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	3	80	theme	flat	480:483	arg1	sheet					485:489	a flat sheet	478:489	a flat sheet	478:489	With regard to structural characterization, SDF from grapefruit peel IDF (GP-IDF-SDF) presented as a flat sheet with cracks, composed of a typical cellulose type I crystal, and had good stability below 200 °C. Galacturonic acid, arabinose and glucuronic acid were the main monosaccharide compositions, indicating that pectin might have been the principal component.
36429327	8	81	with	color	1305:1309	arg1	GP-IDF-SDF					1350:1359	GP-IDF-SDF	1350:1359	GP-IDF-SDF	1350:1359	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	8	81	with	color	1305:1309	arg1	pectin					1364:1369	pectin	1364:1369	pectin	1364:1369	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	2	82	mod	modified	306:313	arg3	method					357:362	the combined microwave and enzymatic method	320:362	the combined microwave and enzymatic method to obtain SDF	320:376	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	2	82	mod	modified	306:313	arg1	fiber					287:291	grapefruit peel insoluble dietary fiber	253:291	grapefruit peel insoluble dietary fiber (GP-IDF)	253:300	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	2	82	mod	modified	306:313	arg1	GP-IDF					294:299	GP-IDF	294:299	GP-IDF	294:299	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	5	83	theme	jam	1061:1063	arg1	texture					1017:1023	texture	1017:1023	texture	1017:1023	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	5	83	theme	jam	1061:1063	arg1	properties					1037:1046	sensory properties	1029:1046	sensory properties	1029:1046	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	5	83	theme	jam	1061:1063	arg1	rheology					1007:1014	rheology	1007:1014	rheology	1007:1014	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	5	83	theme	jam	1061:1063	arg1	color					1000:1004	color	1000:1004	color	1000:1004	Thereafter, the effects of GP-IDF-SDF and commercial pectin addition on the color, rheology, texture and sensory properties of blueberry jam were compared.
36429327	9	84	theme	positive	1470:1477	arg1	role					1479:1482	a positive role	1468:1482	a positive role	1468:1482	Overall, GP-IDF-SDF had functional characteristics and played a positive role in jam, and it is expected to be a candidate for the development of functional food ingredients.
36429327	2	85	theme	enzymatic	347:355	arg1	method					357:362	the combined microwave and enzymatic method	320:362	the combined microwave and enzymatic method to obtain SDF	320:376	In this study, grapefruit peel insoluble dietary fiber (GP-IDF) was modified with the combined microwave and enzymatic method to obtain SDF.
36429327	8	86	theme	sensory	1287:1293	arg1	data					1295:1298	sensory data	1287:1298	sensory data	1287:1298	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	8	87	with	spreadability	1324:1336	arg1	GP-IDF-SDF					1350:1359	GP-IDF-SDF	1350:1359	GP-IDF-SDF	1350:1359	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36429327	8	87	with	spreadability	1324:1336	arg1	pectin					1364:1369	pectin	1364:1369	pectin	1364:1369	According to sensory data, the color, texture and spreadability of jam with GP-IDF-SDF or pectin were improved and more acceptable.
36604449	7	0	theme	host	1016:1019	arg1	colonisation					1021:1032	host colonisation	1016:1032	host colonisation	1016:1032	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	2	1	theme	poultry	356:362	arg1	industry					364:371	the global poultry industry	345:371	the global poultry industry	345:371	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	2	2	theme	global	349:354	arg1	industry					364:371	the global poultry industry	345:371	the global poultry industry	345:371	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	7	3	contain	possesses	1043:1051	arg2	glycoproteins					1060:1072	unique glycoproteins	1053:1072	unique glycoproteins which may contribute to its ability to survive in challenging host environments	1053:1152	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	7	3	contain	possesses	1043:1051	arg1	hepaticus					932:940	C. hepaticus	929:940	C. hepaticus	929:940	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	0	4	from	Characterisation	0:15	arg1	hepaticus					91:99	the bacterial pathogen Campylobacter hepaticus	54:99	the bacterial pathogen Campylobacter hepaticus	54:99	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
36604449	3	5	dep	pathogen	384:391	arg1	jejuni					407:412	Campylobacter jejuni	393:412	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	0	6	link	N-linked	20:27	arg1	glycosylation					37:49	N-linked protein glycosylation	20:49	N-linked protein glycosylation	20:49	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
36604449	8	7	theme	important	1203:1211	arg1	factor					1223:1228	an important virulence factor	1200:1228	an important virulence factor	1200:1228	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	8	7	theme	important	1203:1211	arg1	N-glycosylation					1168:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	4	8	theme	Campylobacter	635:647	arg1	species					649:655	Campylobacter species	635:655	Campylobacter species	635:655	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	2	9	from	increase	228:235	arg1	production					284:293	egg production	280:293	egg production	280:293	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	2	9	from	increase	228:235	arg1	mortality					240:248	mortality	240:248	mortality	240:248	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	7	10	theme	host	1136:1139	arg1	environments					1141:1152	challenging host environments	1124:1152	challenging host environments	1124:1152	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	1	11	from	Disease	177:183	arg1	chickens					200:207	layer chickens	194:207	layer chickens	194:207	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	2	12	theme	egg	280:282	arg1	production					284:293	egg production	280:293	egg production	280:293	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	3	13	theme	glycosylation	434:446	arg1	pathogen					384:391	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	3	13	theme	glycosylation	434:446	arg1	system					448:453	an N-linked glycosylation system	422:453	an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity	422:521	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	5	14	theme	hepaticus	794:802	arg1	system					820:825	the C. hepaticus N-glycosylation system	787:825	the C. hepaticus N-glycosylation system	787:825	In this study glycoproteomic analysis was used to confirm the functionality of the C. hepaticus N-glycosylation system.
36604449	6	15	theme	heptasaccharide	905:919	arg1	glycan					921:926	an N-linked heptasaccharide glycan	893:926	an N-linked heptasaccharide glycan	893:926	It was shown that C. hepaticus HV10T modifies > 35 proteins with an N-linked heptasaccharide glycan.
36604449	1	16	theme	Campylobacter	102:114	arg1	pathogen					142:149	an important pathogen	129:149	an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens	129:207	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	1	16	theme	Campylobacter	102:114	arg1	hepaticus					116:124	Campylobacter hepaticus	102:124	Campylobacter hepaticus	102:124	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	4	17	gly	glycosylation	539:551	arg1	jejuni					588:593	C. jejuni	585:593	C. jejuni	585:593	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	4	17	gly	glycosylation	539:551	arg2	range					626:630	a range	624:630	a range of Campylobacter species	624:655	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	8	18	theme	better	1285:1290	arg1	understanding					1292:1304	a better understanding	1283:1304	a better understanding	1283:1304	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	6	19	link	N-linked	896:903	arg1	glycan					921:926	an N-linked heptasaccharide glycan	893:926	an N-linked heptasaccharide glycan	893:926	It was shown that C. hepaticus HV10T modifies > 35 proteins with an N-linked heptasaccharide glycan.
36604449	6	20	theme	N-linked	896:903	arg1	glycan					921:926	an N-linked heptasaccharide glycan	893:926	an N-linked heptasaccharide glycan	893:926	It was shown that C. hepaticus HV10T modifies > 35 proteins with an N-linked heptasaccharide glycan.
36604449	7	21	theme	C.	985:986	arg1	jejuni					988:993	C. jejuni	985:993	C. jejuni that are involved in host colonisation	985:1032	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	2	22	theme	important	315:323	arg1	concern					334:340	an important economic concern	312:340	an important economic concern of the global poultry industry	312:371	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	1	23	theme	layer	194:198	arg1	chickens					200:207	layer chickens	194:207	layer chickens	194:207	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	3	24	theme	Campylobacter	393:405	arg1	jejuni					407:412	Campylobacter jejuni	393:412	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	7	25	theme	challenging	1124:1134	arg1	environments					1141:1152	challenging host environments	1124:1152	challenging host environments	1124:1152	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	7	26	theme	conserved	956:964	arg1	glycoproteins					966:978	highly conserved glycoproteins	949:978	highly conserved glycoproteins	949:978	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	0	27	theme	protein	29:35	arg1	glycosylation					37:49	N-linked protein glycosylation	20:49	N-linked protein glycosylation	20:49	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
36604449	4	28	theme	C.	585:586	arg1	jejuni					588:593	C. jejuni	585:593	C. jejuni	585:593	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	5	29	theme	N-glycosylation	804:818	arg1	system					820:825	the C. hepaticus N-glycosylation system	787:825	the C. hepaticus N-glycosylation system	787:825	In this study glycoproteomic analysis was used to confirm the functionality of the C. hepaticus N-glycosylation system.
36604449	4	30	theme	species	649:655	arg1	range					626:630	a range	624:630	a range of Campylobacter species	624:655	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	0	31	theme	N-linked	20:27	arg1	glycosylation					37:49	N-linked protein glycosylation	20:49	N-linked protein glycosylation	20:49	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
36604449	4	32	link	N-linked	530:537	arg1	glycosylation					539:551	N-linked glycosylation	530:551	N-linked glycosylation	530:551	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	1	33	theme	important	132:140	arg1	pathogen					142:149	an important pathogen	129:149	an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens	129:207	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	1	33	theme	important	132:140	arg1	hepaticus					116:124	Campylobacter hepaticus	102:124	Campylobacter hepaticus	102:124	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	2	34	from	decrease	268:275	arg1	production					284:293	egg production	280:293	egg production	280:293	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	2	34	from	decrease	268:275	arg1	mortality					240:248	mortality	240:248	mortality	240:248	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	3	35	theme	host	487:490	arg1	colonization					492:503	host colonization	487:503	host colonization	487:503	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	5	36	theme	C.	791:792	arg1	system					820:825	the C. hepaticus N-glycosylation system	787:825	the C. hepaticus N-glycosylation system	787:825	In this study glycoproteomic analysis was used to confirm the functionality of the C. hepaticus N-glycosylation system.
36604449	4	37	theme	C.	680:681	arg1	glycosylation					693:705	C. hepaticus glycosylation	680:705	C. hepaticus glycosylation	680:705	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	5	38	theme	glycoproteomic	722:735	arg1	analysis					737:744	glycoproteomic analysis	722:744	glycoproteomic analysis	722:744	In this study glycoproteomic analysis was used to confirm the functionality of the C. hepaticus N-glycosylation system.
36604449	8	39	theme	poultry	1327:1333	arg1	infection					1335:1343	poultry infection	1327:1343	poultry infection	1327:1343	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	3	40	theme	fundamental	466:476	arg1	roles					478:482	fundamental roles	466:482	fundamental roles	466:482	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	0	41	theme	glycosylation	37:49	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.	0:100	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
36604449	7	42	gly	glycoproteins	1060:1072	arg1	glycoproteins					1060:1072	unique glycoproteins	1053:1072	unique glycoproteins which may contribute to its ability to survive in challenging host environments	1053:1152	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	3	43	theme	N-linked	425:432	arg1	pathogen					384:391	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	3	43	theme	N-linked	425:432	arg1	system					448:453	an N-linked glycosylation system	422:453	an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity	422:521	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	7	44	theme	unique	1053:1058	arg1	glycoproteins					1060:1072	unique glycoproteins	1053:1072	unique glycoproteins which may contribute to its ability to survive in challenging host environments	1053:1152	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	0	45	theme	bacterial	58:66	arg1	hepaticus					91:99	the bacterial pathogen Campylobacter hepaticus	54:99	the bacterial pathogen Campylobacter hepaticus	54:99	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
36604449	8	46	theme	virulence	1213:1221	arg1	factor					1223:1228	an important virulence factor	1200:1228	an important virulence factor	1200:1228	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	8	46	theme	virulence	1213:1221	arg1	N-glycosylation					1168:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	8	47	theme	C.	1155:1156	arg1	factor					1223:1228	an important virulence factor	1200:1228	an important virulence factor	1200:1228	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	8	47	theme	C.	1155:1156	arg1	N-glycosylation					1168:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	6	48	theme	modifies > 35	865:877	arg1	proteins					879:886	modifies > 35 proteins	865:886	modifies > 35 proteins	865:886	It was shown that C. hepaticus HV10T modifies > 35 proteins with an N-linked heptasaccharide glycan.
36604449	5	49	theme	system	820:825	arg1	functionality					770:782	the functionality	766:782	the functionality of the C. hepaticus N-glycosylation system	766:825	In this study glycoproteomic analysis was used to confirm the functionality of the C. hepaticus N-glycosylation system.
36604449	3	50	link	N-linked	425:432	arg1	pathogen					384:391	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	3	50	link	N-linked	425:432	arg1	system					448:453	an N-linked glycosylation system	422:453	an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity	422:521	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	5	51	used	used	750:753	arg2	analysis					737:744	glycoproteomic analysis	722:744	glycoproteomic analysis	722:744	In this study glycoproteomic analysis was used to confirm the functionality of the C. hepaticus N-glycosylation system.
36604449	4	52	theme	hepaticus	683:691	arg1	glycosylation					693:705	C. hepaticus glycosylation	680:705	C. hepaticus glycosylation	680:705	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	2	53	dep	important	315:323	arg1	economic					325:332	economic	325:332	economic	325:332	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	8	54	theme	hepaticus	1158:1166	arg1	factor					1223:1228	an important virulence factor	1200:1228	an important virulence factor	1200:1228	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	8	54	theme	hepaticus	1158:1166	arg1	N-glycosylation					1168:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation	1155:1182	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	2	55	theme	significant	256:266	arg1	decrease					268:275	a significant decrease	254:275	a significant decrease in egg production	254:293	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
36604449	1	56	theme	Spotty	164:169	arg1	Disease					177:183	Spotty Liver Disease	164:183	Spotty Liver Disease (SLD) in layer chickens	164:207	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	1	56	theme	Spotty	164:169	arg1	SLD					186:188	SLD	186:188	SLD	186:188	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	1	57	theme	Liver	171:175	arg1	Disease					177:183	Spotty Liver Disease	164:183	Spotty Liver Disease (SLD) in layer chickens	164:207	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	1	57	theme	Liver	171:175	arg1	SLD					186:188	SLD	186:188	SLD	186:188	Campylobacter hepaticus is an important pathogen which causes Spotty Liver Disease (SLD) in layer chickens.
36604449	7	58	gly	glycoproteins	966:978	arg1	glycoproteins					966:978	highly conserved glycoproteins	949:978	highly conserved glycoproteins	949:978	C. hepaticus shares highly conserved glycoproteins with C. jejuni that are involved in host colonisation and also possesses unique glycoproteins which may contribute to its ability to survive in challenging host environments.
36604449	0	59	theme	pathogen Campylobacter	68:89	arg1	hepaticus					91:99	the bacterial pathogen Campylobacter hepaticus	54:99	the bacterial pathogen Campylobacter hepaticus	54:99	Characterisation of N-linked protein glycosylation in the bacterial pathogen Campylobacter hepaticus.
36604449	4	60	theme	N-linked	530:537	arg1	glycosylation					539:551	N-linked glycosylation	530:551	N-linked glycosylation	530:551	While N-linked glycosylation has been extensively studied in C. jejuni and is now known to occur in a range of Campylobacter species, little is known about C. hepaticus glycosylation.
36604449	3	61	theme	human	378:382	arg1	pathogen					384:391	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni	374:412	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	3	61	theme	human	378:382	arg1	system					448:453	an N-linked glycosylation system	422:453	an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity	422:521	The human pathogen Campylobacter jejuni encodes an N-linked glycosylation system that plays fundamental roles in host colonization and pathogenicity.
36604449	8	62	from	role	1319:1322	arg1	infection					1335:1343	poultry infection	1327:1343	poultry infection	1327:1343	C. hepaticus N-glycosylation may function as an important virulence factor, providing an opportunity to investigate and develop a better understanding the system's role in poultry infection.
36604449	2	63	theme	industry	364:371	arg1	concern					334:340	an important economic concern	312:340	an important economic concern of the global poultry industry	312:371	SLD results in an increase in mortality and a significant decrease in egg production and therefore is an important economic concern of the global poultry industry.
37350184	7	0	dep	short	1129:1133	arg1	short					1129:1133	short	1129:1133	short	1129:1133	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	0	dep	short	1129:1133	arg1	~30 min					1141:1147	t1/2 ~30 min	1136:1147	t1/2 ~30 min	1136:1147	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	0	dep	short	1129:1133	arg1	turnover					1098:1105	The intracellular turnover	1080:1105	The intracellular turnover of sAC85	1080:1114	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	8	1	theme	N-linked	1290:1297	arg1	isoform					1316:1322	an N-linked glycosylated sAC isoform	1287:1322	an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1287:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	7	2	theme	sACEV	1165:1169	arg1	release					1154:1160	release	1154:1160	release of sACEV in the medium	1154:1183	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	8	3	dep	maturation	1239:1248	arg1	the					1235:1237	the	1235:1237	the	1235:1237	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	6	4	theme	vesicular	953:961	arg1	machinery					963:971	the vesicular machinery	949:971	the vesicular machinery	949:971	Consistently, disrupting the vesicular machinery affects the maturation of intracellular sAC and inhibits the release of sACEV into extracellular vesicles.
37350184	7	5	theme	t1/2	1136:1139	arg1	short					1129:1133	short	1129:1133	short	1129:1133	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	5	theme	t1/2	1136:1139	arg1	~30 min					1141:1147	t1/2 ~30 min	1136:1147	t1/2 ~30 min	1136:1147	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	5	theme	t1/2	1136:1139	arg1	turnover					1098:1105	The intracellular turnover	1080:1105	The intracellular turnover of sAC85	1080:1114	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	6	located	detected	1189:1196	arg1	3 h					1205:1207	3 h	1205:1207	3 h	1205:1207	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	6	located	detected	1189:1196	arg2	release					1154:1160	release	1154:1160	release of sACEV in the medium	1154:1183	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	2	7	theme	75 kD	345:349	arg1	band					372:375	a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band	325:375	band	372:375	We consistently observed a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band under glucose-sufficient or glucose-deprived states, respectively, in H69 cholangiocytes by immunoblotting.
37350184	1	8	theme	sAC	258:260	arg1	levels					270:275	sAC protein levels	258:275	sAC protein levels	258:275	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	5	9	theme	sAC	763:765	arg1	release					730:736	release	730:736	release of N-linked glycosylated sAC (sACEV )	730:774	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	1	10	theme	protein	262:268	arg1	levels					270:275	sAC protein levels	258:275	sAC protein levels	258:275	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	5	11	link	N-linked	741:748	arg1	sACEV					768:772	sACEV	768:772	sACEV	768:772	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	5	11	link	N-linked	741:748	arg1	sAC					763:765	N-linked glycosylated sAC	741:765	N-linked glycosylated sAC (sACEV )	741:774	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	3	12	theme	N-linked	556:563	arg1	proteins					578:585	N-linked glycosylated proteins	556:585	N-linked glycosylated proteins with the same polypeptide backbone	556:620	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	12	theme	N-linked	556:563	arg1	sAC85					546:550	sAC85	546:550	sAC85	546:550	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	12	theme	N-linked	556:563	arg1	sAC75					536:540	sAC75	536:540	sAC75	536:540	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	13	gly	glycosylated	565:576	arg1	proteins					578:585	N-linked glycosylated proteins	556:585	N-linked glycosylated proteins with the same polypeptide backbone	556:620	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	13	gly	glycosylated	565:576	arg1	sAC85					546:550	sAC85	546:550	sAC85	546:550	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	13	gly	glycosylated	565:576	arg1	sAC75					536:540	sAC75	536:540	sAC75	536:540	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	0	14	theme	extracellular	108:120	arg1	vesicles					122:129	extracellular vesicles	108:129	extracellular vesicles	108:129	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	8	15	theme	sAC	1312:1314	arg1	isoform					1316:1322	an N-linked glycosylated sAC isoform	1287:1322	an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1287:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	4	16	theme	sugar	704:708	arg1	chains					710:715	distinct sugar chains	695:715	distinct sugar chains	695:715	Deglycosylation with Endo-H further revealed that sAC75 and sAC85 carry distinct sugar chains.
37350184	1	17	theme	-derived	162:169	arg1	cAMP					171:174	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	0	18	theme	adenylyl	45:52	arg1	cyclase					54:60	soluble adenylyl cyclase	37:60	soluble adenylyl cyclase	37:60	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	8	19	from	trafficking	1254:1264	arg1	cholangiocytes					1269:1282	cholangiocytes	1269:1282	cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1269:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	0	20	gly	glycosylated	79:90	arg1	N-linked					70:77	N-linked	70:77	N-linked	70:77	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	20	gly	glycosylated	79:90	arg1	secreted					96:103	secreted	96:103	secreted in extracellular vesicles	96:129	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	20	gly	glycosylated	79:90	arg1	isoform					26:32	an isoform	23:32	an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles	23:129	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	20	gly	glycosylated	79:90	arg1	glycosylated					79:90	glycosylated	79:90	glycosylated	79:90	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	5	21	theme	glucose-deprived	894:909	arg1	conditions					912:921	sAC75 (glucose-deprived) conditions	887:921	sAC75 (glucose-deprived) conditions	887:921	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	1	22	link	-derived	162:169	arg1	cAMP					171:174	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	2	23	theme	protein	364:370	arg1	band					372:375	a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band	325:375	band	372:375	We consistently observed a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band under glucose-sufficient or glucose-deprived states, respectively, in H69 cholangiocytes by immunoblotting.
37350184	3	24	with	proteins	578:585	arg1	backbone					613:620	the same polypeptide backbone	592:620	the same polypeptide backbone	592:620	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	0	25	dep	N-linked	70:77	arg1	N-linked					70:77	N-linked	70:77	N-linked	70:77	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	25	dep	N-linked	70:77	arg1	secreted					96:103	secreted	96:103	secreted in extracellular vesicles	96:129	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	25	dep	N-linked	70:77	arg1	glycosylated					79:90	glycosylated	79:90	glycosylated	79:90	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	25	dep	N-linked	70:77	arg1	isoform					26:32	an isoform	23:32	an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles	23:129	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	2	26	theme	sAC	360:362	arg1	band					372:375	a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band	325:375	band	372:375	We consistently observed a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band under glucose-sufficient or glucose-deprived states, respectively, in H69 cholangiocytes by immunoblotting.
37350184	8	27	theme	extracellular	1354:1366	arg1	vesicles					1368:1375	extracellular vesicles	1354:1375	extracellular vesicles	1354:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	4	28	with	Deglycosylation	623:637	arg1	Endo-H					644:649	Endo-H	644:649	Endo-H	644:649	Deglycosylation with Endo-H further revealed that sAC75 and sAC85 carry distinct sugar chains.
37350184	3	29	theme	glycosylated	565:576	arg1	proteins					578:585	N-linked glycosylated proteins	556:585	N-linked glycosylated proteins with the same polypeptide backbone	556:620	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	29	theme	glycosylated	565:576	arg1	sAC85					546:550	sAC85	546:550	sAC85	546:550	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	29	theme	glycosylated	565:576	arg1	sAC75					536:540	sAC75	536:540	sAC75	536:540	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	1	30	theme	cellular	194:201	arg1	processes					203:211	various cellular processes	186:211	various cellular processes	186:211	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	2	31	theme	glucose-deprived	405:420	arg1	states					422:427	glucose-sufficient or glucose-deprived states	383:427	glucose-sufficient or glucose-deprived states	383:427	We consistently observed a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band under glucose-sufficient or glucose-deprived states, respectively, in H69 cholangiocytes by immunoblotting.
37350184	0	32	theme	soluble	37:43	arg1	cyclase					54:60	soluble adenylyl cyclase	37:60	soluble adenylyl cyclase	37:60	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	6	33	theme	sAC	1013:1015	arg1	maturation					985:994	the maturation	981:994	the maturation of intracellular sAC	981:1015	Consistently, disrupting the vesicular machinery affects the maturation of intracellular sAC and inhibits the release of sACEV into extracellular vesicles.
37350184	5	34	theme	N-linked	741:748	arg1	sACEV					768:772	sACEV	768:772	sACEV	768:772	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	5	34	theme	N-linked	741:748	arg1	sAC					763:765	N-linked glycosylated sAC	741:765	N-linked glycosylated sAC (sACEV )	741:774	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	8	35	link	N-linked	1290:1297	arg1	isoform					1316:1322	an N-linked glycosylated sAC isoform	1287:1322	an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1287:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	1	36	theme	various	186:192	arg1	processes					203:211	various cellular processes	186:211	various cellular processes	186:211	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	6	37	theme	intracellular	999:1011	arg1	sAC					1013:1015	intracellular sAC	999:1015	intracellular sAC	999:1015	Consistently, disrupting the vesicular machinery affects the maturation of intracellular sAC and inhibits the release of sACEV into extracellular vesicles.
37350184	5	38	theme	glycosylated	750:761	arg1	sACEV					768:772	sACEV	768:772	sACEV	768:772	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	5	38	theme	glycosylated	750:761	arg1	sAC					763:765	N-linked glycosylated sAC	741:765	N-linked glycosylated sAC (sACEV )	741:774	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	6	39	theme	sACEV	1045:1049	arg1	release					1034:1040	the release	1030:1040	the release of sACEV into extracellular vesicles	1030:1077	Consistently, disrupting the vesicular machinery affects the maturation of intracellular sAC and inhibits the release of sACEV into extracellular vesicles.
37350184	5	40	theme	sAC75	887:891	arg1	conditions					912:921	sAC75 (glucose-deprived) conditions	887:921	sAC75 (glucose-deprived) conditions	887:921	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	2	41	theme	glucose-sufficient	383:400	arg1	states					422:427	glucose-sufficient or glucose-deprived states	383:427	glucose-sufficient or glucose-deprived states	383:427	We consistently observed a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band under glucose-sufficient or glucose-deprived states, respectively, in H69 cholangiocytes by immunoblotting.
37350184	0	42	theme	cyclase	54:60	arg1	N-linked					70:77	N-linked	70:77	N-linked	70:77	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	42	theme	cyclase	54:60	arg1	secreted					96:103	secreted	96:103	secreted in extracellular vesicles	96:129	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	42	theme	cyclase	54:60	arg1	isoform					26:32	an isoform	23:32	an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles	23:129	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	0	42	theme	cyclase	54:60	arg1	glycosylated					79:90	glycosylated	79:90	glycosylated	79:90	Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.
37350184	7	43	from	release	1154:1160	arg1	medium					1178:1183	the medium	1174:1183	the medium	1174:1183	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	5	44	theme	intracellular	832:844	arg1	glucose-sufficient					853:870	glucose-sufficient	853:870	glucose-sufficient	853:870	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	5	44	theme	intracellular	832:844	arg1	sAC85					846:850	intracellular sAC85	832:850	intracellular sAC85 (glucose-sufficient)	832:871	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	8	45	gly	glycosylated	1299:1310	arg1	isoform					1316:1322	an N-linked glycosylated sAC isoform	1287:1322	an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1287:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	3	46	theme	same	596:599	arg1	backbone					613:620	the same polypeptide backbone	592:620	the same polypeptide backbone	592:620	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	2	47	theme	H69	447:449	arg1	cholangiocytes					451:464	H69 cholangiocytes	447:464	H69 cholangiocytes	447:464	We consistently observed a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band under glucose-sufficient or glucose-deprived states, respectively, in H69 cholangiocytes by immunoblotting.
37350184	7	48	theme	sAC85	1110:1114	arg1	short					1129:1133	short	1129:1133	short	1129:1133	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	48	theme	sAC85	1110:1114	arg1	turnover					1098:1105	The intracellular turnover	1080:1105	The intracellular turnover of sAC85	1080:1114	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	48	theme	sAC85	1110:1114	arg1	~30 min					1141:1147	t1/2 ~30 min	1136:1147	t1/2 ~30 min	1136:1147	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	3	49	theme	polypeptide	601:611	arg1	backbone					613:620	the same polypeptide backbone	592:620	the same polypeptide backbone	592:620	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	4	50	theme	distinct	695:702	arg1	chains					710:715	distinct sugar chains	695:715	distinct sugar chains	695:715	Deglycosylation with Endo-H further revealed that sAC75 and sAC85 carry distinct sugar chains.
37350184	7	51	theme	intracellular	1084:1096	arg1	short					1129:1133	short	1129:1133	short	1129:1133	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	51	theme	intracellular	1084:1096	arg1	turnover					1098:1105	The intracellular turnover	1080:1105	The intracellular turnover of sAC85	1080:1114	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	7	51	theme	intracellular	1084:1096	arg1	~30 min					1141:1147	t1/2 ~30 min	1136:1147	t1/2 ~30 min	1136:1147	The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h.
37350184	8	52	theme	glycosylated	1299:1310	arg1	isoform					1316:1322	an N-linked glycosylated sAC isoform	1287:1322	an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1287:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	1	53	theme	Soluble	132:138	arg1	sAC					158:160	sAC	158:160	sAC	158:160	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	1	53	theme	Soluble	132:138	arg1	cyclase					149:155	Soluble adenylyl cyclase	132:155	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	3	54	link	N-linked	556:563	arg1	proteins					578:585	N-linked glycosylated proteins	556:585	N-linked glycosylated proteins with the same polypeptide backbone	556:620	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	54	link	N-linked	556:563	arg1	sAC85					546:550	sAC85	546:550	sAC85	546:550	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	3	54	link	N-linked	556:563	arg1	sAC75					536:540	sAC75	536:540	sAC75	536:540	Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone.
37350184	1	55	theme	adenylyl	140:147	arg1	sAC					158:160	sAC	158:160	sAC	158:160	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	1	55	theme	adenylyl	140:147	arg1	cyclase					149:155	Soluble adenylyl cyclase	132:155	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	5	56	gly	glycosylated	750:761	arg1	sACEV					768:772	sACEV	768:772	sACEV	768:772	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	5	56	gly	glycosylated	750:761	arg1	sAC					763:765	N-linked glycosylated sAC	741:765	N-linked glycosylated sAC (sACEV )	741:774	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	4	57	contain	carry	689:693	arg1	sAC75					673:677	sAC75	673:677	sAC75	673:677	Deglycosylation with Endo-H further revealed that sAC75 and sAC85 carry distinct sugar chains.
37350184	4	57	contain	carry	689:693	arg2	chains					710:715	distinct sugar chains	695:715	distinct sugar chains	695:715	Deglycosylation with Endo-H further revealed that sAC75 and sAC85 carry distinct sugar chains.
37350184	4	57	contain	carry	689:693	arg1	sAC85					683:687	sAC85	683:687	sAC85	683:687	Deglycosylation with Endo-H further revealed that sAC75 and sAC85 carry distinct sugar chains.
37350184	8	58	theme	isoform	1316:1322	arg1	cholangiocytes					1269:1282	cholangiocytes	1269:1282	cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1269:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	1	59	theme	cyclase	149:155	arg1	cAMP					171:174	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP	132:174	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	1	60	theme	regulatory	227:236	arg1	landscape					238:246	the regulatory landscape	223:246	the regulatory landscape mediating sAC protein levels	223:275	Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored.
37350184	5	61	theme	extracellular	779:791	arg1	vesicles					793:800	extracellular vesicles	779:800	extracellular vesicles	779:800	We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions.
37350184	8	62	from	maturation	1239:1248	arg1	cholangiocytes					1269:1282	cholangiocytes	1269:1282	cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles	1269:1375	Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.
37350184	6	63	theme	extracellular	1056:1068	arg1	vesicles					1070:1077	extracellular vesicles	1056:1077	extracellular vesicles	1056:1077	Consistently, disrupting the vesicular machinery affects the maturation of intracellular sAC and inhibits the release of sACEV into extracellular vesicles.
36737137	9	0	theme	HILIC	1694:1698	arg1	material					1700:1707	one additional HILIC material	1679:1707	one additional HILIC material for the enrichment of glycopeptides	1679:1743	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	6	1	theme	protein	1209:1215	arg1	digests					1217:1223	serum protein digests	1203:1223	serum protein digests	1203:1223	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	3	2	theme	mixture	585:591	arg1	amount					567:572	the amount	563:572	the amount of porogen mixture	563:591	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	2	theme	mixture	585:591	arg1	mixture					585:591	porogen mixture	577:591	porogen mixture	577:591	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	2	theme	mixture	585:591	arg1	amount					502:507	the amount	498:507	the amount of monomer-to-crosslinker ratio	498:539	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	2	theme	mixture	585:591	arg1	ratio					535:539	monomer-to-crosslinker ratio	512:539	monomer-to-crosslinker ratio	512:539	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	2	theme	mixture	585:591	arg1	composition					546:556	the composition	542:556	the composition	542:556	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	5	3	theme	monomer	912:918	arg1	percent					920:926	a monomer percent	910:926	a monomer percent	910:926	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	9	4	theme	glycopeptides	1731:1743	arg1	enrichment					1717:1726	the enrichment	1713:1726	the enrichment of glycopeptides	1713:1743	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	7	5	theme	77	1431:1432	arg1	%					1433:1433	%	1433:1433	%	1433:1433	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	0	6	theme	protein	115:121	arg1	digests					123:129	serum protein digests	109:129	serum protein digests	109:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	1	7	theme	HILIC	198:202	arg1	material					204:211	a new HILIC material	192:211	a new HILIC material for the enrichment of N-linked glycopeptides	192:256	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	0	8	from	enrichment	69:78	arg1	digests					123:129	serum protein digests	109:129	serum protein digests	109:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	8	9	theme	monomer	1497:1503	arg1	amount					1487:1492	a high amount	1480:1492	a high amount of monomer	1480:1503	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	8	9	theme	monomer	1497:1503	arg1	monomer					1497:1503	monomer	1497:1503	monomer	1497:1503	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	9	10	link	N-linked	1839:1846	arg1	glycopeptides					1848:1860	N-linked glycopeptides	1839:1860	N-linked glycopeptides	1839:1860	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	2	11	dep	monomer	343:349	arg1	dimethacrylate					371:384	dimethacrylate	371:384	dimethacrylate	371:384	The material was prepared using 2-acrylamido-2-methyl-1-propanesulfonic acid as the monomer and ethylene glycol dimethacrylate as the cross-linker.
36737137	3	12	theme	porogen	577:583	arg1	mixture					585:591	porogen mixture	577:591	porogen mixture	577:591	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	8	13	gly	glycopeptides	1551:1563	arg2	glycopeptides					1551:1563	enriched glycopeptides	1542:1563	enriched glycopeptides	1542:1563	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	6	14	theme	new	1046:1048	arg1	family					1058:1063	this new polymer family	1041:1063	this new polymer family	1041:1063	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	7	15	theme	selectivity	1370:1380	arg1	terms					1311:1315	terms	1311:1315	terms of the number of enriched N-linked glycopeptides and selectivity	1311:1380	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	6	16	from	digests	1217:1223	arg1	enrichment					1170:1179	the enrichment	1166:1179	the enrichment of glycopeptides from serum protein digests	1166:1223	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	9	17	theme	hydrogel	1798:1805	arg1	materials					1807:1815	hydrogel materials	1798:1815	hydrogel materials	1798:1815	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	8	18	theme	enriched	1542:1549	arg1	glycopeptides					1551:1563	enriched glycopeptides	1542:1563	enriched glycopeptides	1542:1563	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	7	19	dep	enrichment	1291:1300	arg1	both					1303:1306	both	1303:1306	both	1303:1306	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	0	20	theme	intact	83:88	arg1	glycopeptides					90:102	intact glycopeptides	83:102	intact glycopeptides from serum protein digests	83:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	9	21	gly	glycopeptides	1848:1860	arg2	glycopeptides					1848:1860	N-linked glycopeptides	1839:1860	N-linked glycopeptides	1839:1860	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	5	22	theme	swelling	987:994	arg1	capacities					996:1005	very different water swelling capacities	966:1005	very different water swelling capacities	966:1005	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	7	23	gly	glycopeptides	1352:1364	arg2	glycopeptides					1352:1364	enriched N-linked glycopeptides	1334:1364	enriched N-linked glycopeptides	1334:1364	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	6	24	from	potential	1028:1036	arg1	digests					1075:1081	fetuin digests	1068:1081	fetuin digests	1068:1081	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	0	25	theme	selective	59:67	arg1	enrichment					69:78	the selective enrichment	55:78	the selective enrichment of intact glycopeptides from serum protein digests	55:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	0	26	theme	New	0:2	arg1	material					16:23	New hydrophilic material	0:23	New hydrophilic material	0:23	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	0	27	gly	glycopeptides	90:102	arg2	glycopeptides					90:102	intact glycopeptides	83:102	intact glycopeptides from serum protein digests	83:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	0	27	gly	glycopeptides	90:102	arg1	digests					123:129	serum protein digests	109:129	serum protein digests	109:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	7	28	theme	N-linked	1343:1350	arg1	glycopeptides					1352:1364	enriched N-linked glycopeptides	1334:1364	enriched N-linked glycopeptides	1334:1364	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	9	29	theme	N-linked	1839:1846	arg1	glycopeptides					1848:1860	N-linked glycopeptides	1839:1860	N-linked glycopeptides	1839:1860	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	5	30	theme	important	852:860	arg1	function					862:869	an important function	849:869	an important function	849:869	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	5	31	theme	different	971:979	arg1	capacities					996:1005	very different water swelling capacities	966:1005	very different water swelling capacities	966:1005	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	6	32	theme	fetuin	1068:1073	arg1	digests					1075:1081	fetuin digests	1068:1081	fetuin digests	1068:1081	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	3	33	theme	Box-Behnken	439:449	arg1	design					464:469	a Box-Behnken experimental design	437:469	a Box-Behnken experimental design	437:469	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	5	34	theme	swelling	819:826	arg1	swelling					819:826	swelling	819:826	swelling	819:826	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	5	34	theme	swelling	819:826	arg1	amount					809:814	the amount	805:814	the amount of swelling	805:826	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	3	35	theme	experimental	451:462	arg1	design					464:469	a Box-Behnken experimental design	437:469	a Box-Behnken experimental design	437:469	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	6	36	theme	family	1058:1063	arg1	potential					1028:1036	the potential	1024:1036	the potential of this new polymer family on fetuin digests	1024:1081	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	8	37	theme	hydrogel	1607:1614	arg1	nature					1616:1621	the hydrogel nature	1603:1621	the hydrogel nature of the resulting polymers	1603:1647	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	3	38	theme	monomer-to-crosslinker	512:533	arg1	ratio					535:539	monomer-to-crosslinker ratio	512:539	monomer-to-crosslinker ratio	512:539	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	6	39	theme	experimental	1132:1143	arg1	design					1145:1150	the Box-Behnken experimental design	1116:1150	the Box-Behnken experimental design	1116:1150	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	8	40	theme	resulting	1630:1638	arg1	polymers					1640:1647	the resulting polymers	1626:1647	the resulting polymers	1626:1647	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	4	41	theme	polymer	642:648	arg1	property					616:623	the property	612:623	the property of the resulting polymer	612:648	By this approach, the property of the resulting polymer could be fine-tuned to modulate the hydrophilicity and porosity.
36737137	1	42	theme	glycopeptides	244:256	arg1	enrichment					221:230	the enrichment	217:230	the enrichment of N-linked glycopeptides	217:256	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	9	43	theme	additional	1683:1692	arg1	material					1700:1707	one additional HILIC material	1679:1707	one additional HILIC material for the enrichment of glycopeptides	1679:1743	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	7	44	theme	cotton	1278:1283	arg1	enrichment					1291:1300	cotton HILIC enrichment	1278:1300	cotton HILIC enrichment	1278:1300	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	2	45	theme	2-acrylamido-2-methyl-1-propanesulfonic	291:329	arg1	cross-linker					393:404	the cross-linker	389:404	the cross-linker	389:404	The material was prepared using 2-acrylamido-2-methyl-1-propanesulfonic acid as the monomer and ethylene glycol dimethacrylate as the cross-linker.
36737137	2	45	theme	2-acrylamido-2-methyl-1-propanesulfonic	291:329	arg1	acid					331:334	2-acrylamido-2-methyl-1-propanesulfonic acid	291:334	2-acrylamido-2-methyl-1-propanesulfonic acid	291:334	The material was prepared using 2-acrylamido-2-methyl-1-propanesulfonic acid as the monomer and ethylene glycol dimethacrylate as the cross-linker.
36737137	2	45	theme	2-acrylamido-2-methyl-1-propanesulfonic	291:329	arg1	monomer					343:349	the monomer	339:349	the monomer	339:349	The material was prepared using 2-acrylamido-2-methyl-1-propanesulfonic acid as the monomer and ethylene glycol dimethacrylate as the cross-linker.
36737137	5	46	dep	have	844:847	arg1	considered					899:908	considered	899:908	considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities	899:1005	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	6	47	theme	serum	1203:1207	arg1	digests					1217:1223	serum protein digests	1203:1223	serum protein digests	1203:1223	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	9	48	gly	glycopeptides	1731:1743	arg2	glycopeptides					1731:1743	glycopeptides	1731:1743	glycopeptides	1731:1743	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	7	49	link	N-linked	1343:1350	arg1	glycopeptides					1352:1364	enriched N-linked glycopeptides	1334:1364	enriched N-linked glycopeptides	1334:1364	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	6	50	theme	glycopeptides	1184:1196	arg1	enrichment					1170:1179	the enrichment	1166:1179	the enrichment of glycopeptides from serum protein digests	1166:1223	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	1	51	link	N-linked	235:242	arg1	glycopeptides					244:256	N-linked glycopeptides	235:256	N-linked glycopeptides	235:256	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	1	52	theme	new	194:196	arg1	material					204:211	a new HILIC material	192:211	a new HILIC material for the enrichment of N-linked glycopeptides	192:256	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	8	53	theme	high	1482:1485	arg1	amount					1487:1492	a high amount	1480:1492	a high amount of monomer	1480:1503	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	8	53	theme	high	1482:1485	arg1	monomer					1497:1503	monomer	1497:1503	monomer	1497:1503	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	7	54	theme	%	1433:1433	arg1	selectivity					1435:1445	77% selectivity	1431:1445	77% selectivity	1431:1445	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	9	55	dep	use	1775:1777	arg1	the					1771:1773	the	1771:1773	the	1771:1773	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	1	56	theme	material	204:211	arg1	characterization					172:187	characterization	172:187	characterization	172:187	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	1	56	theme	material	204:211	arg1	preparation					156:166	preparation	156:166	preparation	156:166	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	7	57	gly	glycopeptides	1412:1424	arg2	glycopeptides					1412:1424	762 N-linked glycopeptides	1399:1424	762 N-linked glycopeptides with 77% selectivity	1399:1445	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	5	58	contain	have	844:847	arg2	function					862:869	an important function	849:869	an important function	849:869	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	5	58	contain	have	844:847	arg1	swelling					819:826	swelling	819:826	swelling	819:826	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	5	58	contain	have	844:847	arg1	amount					809:814	the amount	805:814	the amount of swelling	805:826	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	6	59	gly	glycopeptides	1184:1196	arg2	glycopeptides					1184:1196	glycopeptides	1184:1196	glycopeptides	1184:1196	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	5	60	theme	hydrophilic	758:768	arg1	interactions					770:781	hydrophilic interactions	758:781	hydrophilic interactions	758:781	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	5	60	theme	hydrophilic	758:768	arg1	H-bonding					794:802	H-bonding	794:802	H-bonding	794:802	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	7	61	theme	762 N-linked	1399:1410	arg1	glycopeptides					1412:1424	762 N-linked glycopeptides	1399:1424	762 N-linked glycopeptides with 77% selectivity	1399:1445	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	5	62	theme	HILIC	718:722	arg1	enrichment					724:733	HILIC enrichment	718:733	HILIC enrichment	718:733	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	5	63	theme	%	949:949	arg1	range					935:939	the range	931:939	the range	931:939	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	6	64	theme	polymer	1050:1056	arg1	family					1058:1063	this new polymer family	1041:1063	this new polymer family	1041:1063	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	2	65	theme	ethylene	355:362	arg1	glycol					364:369	ethylene glycol	355:369	ethylene glycol	355:369	The material was prepared using 2-acrylamido-2-methyl-1-propanesulfonic acid as the monomer and ethylene glycol dimethacrylate as the cross-linker.
36737137	7	66	theme	superior	1252:1259	arg1	performance					1261:1271	a superior performance	1250:1271	a superior performance	1250:1271	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	4	67	theme	resulting	632:640	arg1	polymer					642:648	the resulting polymer	628:648	the resulting polymer	628:648	By this approach, the property of the resulting polymer could be fine-tuned to modulate the hydrophilicity and porosity.
36737137	6	68	used	used	1157:1160	arg2	materials					1091:1099	the 17 materials	1084:1099	the 17 materials resulting from the Box-Behnken experimental design	1084:1150	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	8	69	theme	glycopeptides	1551:1563	arg1	number					1532:1537	the number	1528:1537	the number	1528:1537	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	0	70	theme	glycopeptides	90:102	arg1	enrichment					69:78	the selective enrichment	55:78	the selective enrichment of intact glycopeptides from serum protein digests	55:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	5	71	theme	water	981:985	arg1	capacities					996:1005	very different water swelling capacities	966:1005	very different water swelling capacities	966:1005	As HILIC enrichment is mostly dependent on hydrophilic interactions, including H-bonding, the amount of swelling was expected to have an important function, therefore the optimization considered a monomer percent in the range of 20-80%, which implied very different water swelling capacities.
36737137	9	72	theme	materials	1807:1815	arg1	development					1783:1793	development	1783:1793	development	1783:1793	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	9	72	theme	materials	1807:1815	arg1	use					1775:1777	use	1775:1777	use	1775:1777	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	0	73	theme	serum	109:113	arg1	digests					123:129	serum protein digests	109:129	serum protein digests	109:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	7	74	theme	glycopeptides	1352:1364	arg1	number					1324:1329	the number	1320:1329	the number of enriched N-linked glycopeptides	1320:1364	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	7	74	theme	glycopeptides	1352:1364	arg1	selectivity					1370:1380	selectivity	1370:1380	selectivity	1370:1380	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	7	75	with	glycopeptides	1412:1424	arg1	selectivity					1435:1445	77% selectivity	1431:1445	77% selectivity	1431:1445	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	0	76	theme	hydrophilic	4:14	arg1	material					16:23	New hydrophilic material	0:23	New hydrophilic material	0:23	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	1	77	gly	glycopeptides	244:256	arg2	glycopeptides					244:256	N-linked glycopeptides	235:256	N-linked glycopeptides	235:256	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	7	78	theme	enriched	1334:1341	arg1	glycopeptides					1352:1364	enriched N-linked glycopeptides	1334:1364	enriched N-linked glycopeptides	1334:1364	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	1	79	dep	preparation	156:166	arg1	the					152:154	the	152:154	the	152:154	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
36737137	7	80	theme	number	1324:1329	arg1	terms					1311:1315	terms	1311:1315	terms of the number of enriched N-linked glycopeptides and selectivity	1311:1380	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	9	81	theme	glycopeptides	1848:1860	arg1	enrichment					1825:1834	the enrichment	1821:1834	the enrichment of N-linked glycopeptides	1821:1860	The results not only provide one additional HILIC material for the enrichment of glycopeptides but also pave the way for the use and development of hydrogel materials for the enrichment of N-linked glycopeptides.
36737137	6	82	theme	Box-Behnken	1120:1130	arg1	design					1145:1150	the Box-Behnken experimental design	1116:1150	the Box-Behnken experimental design	1116:1150	After assessing the potential of this new polymer family on fetuin digests, the 17 materials resulting from the Box-Behnken experimental design were used for the enrichment of glycopeptides from serum protein digests.
36737137	0	83	theme	hydrogel	34:41	arg1	polymer					43:49	hydrogel polymer	34:49	hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests	34:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	7	84	link	762 N-linked	1399:1410	arg1	glycopeptides					1412:1424	762 N-linked glycopeptides	1399:1424	762 N-linked glycopeptides with 77% selectivity	1399:1445	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	8	85	theme	polymers	1640:1647	arg1	nature					1616:1621	the hydrogel nature	1603:1621	the hydrogel nature of the resulting polymers	1603:1647	The optimization indicated that a high amount of monomer significantly affected the number of enriched glycopeptides, which is also closely connected with the hydrogel nature of the resulting polymers.
36737137	3	86	theme	ratio	535:539	arg1	amount					567:572	the amount	563:572	the amount of porogen mixture	563:591	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	86	theme	ratio	535:539	arg1	mixture					585:591	porogen mixture	577:591	porogen mixture	577:591	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	86	theme	ratio	535:539	arg1	amount					502:507	the amount	498:507	the amount of monomer-to-crosslinker ratio	498:539	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	86	theme	ratio	535:539	arg1	ratio					535:539	monomer-to-crosslinker ratio	512:539	monomer-to-crosslinker ratio	512:539	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	3	86	theme	ratio	535:539	arg1	composition					546:556	the composition	542:556	the composition	542:556	The material was developed by a Box-Behnken experimental design, taking into consideration the amount of monomer-to-crosslinker ratio, the composition, and the amount of porogen mixture.
36737137	7	87	theme	HILIC	1285:1289	arg1	enrichment					1291:1300	cotton HILIC enrichment	1278:1300	cotton HILIC enrichment	1278:1300	The materials displayed a superior performance over cotton HILIC enrichment, both in terms of the number of enriched N-linked glycopeptides and selectivity, providing up to 762 N-linked glycopeptides with 77% selectivity.
36737137	0	88	from	digests	123:129	arg1	enrichment					69:78	the selective enrichment	55:78	the selective enrichment of intact glycopeptides from serum protein digests	55:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	0	88	from	digests	123:129	arg1	glycopeptides					90:102	intact glycopeptides	83:102	intact glycopeptides from serum protein digests	83:129	New hydrophilic material based on hydrogel polymer for the selective enrichment of intact glycopeptides from serum protein digests.
36737137	1	89	theme	N-linked	235:242	arg1	glycopeptides					244:256	N-linked glycopeptides	235:256	N-linked glycopeptides	235:256	The paper describes the preparation and characterization of a new HILIC material for the enrichment of N-linked glycopeptides.
35741969	0	0	theme	Chemical	79:86	arg1	Characterization					88:103	the Physical and Chemical Characterization	62:103	the Physical and Chemical Characterization	62:103	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	4	1	from	Zhangii	825:831	arg1	%					857:857	as high as13.63%	842:857	as high as13.63%	842:857	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	4	1	from	Zhangii	825:831	arg1	fucoidan					801:808	crude fucoidan	795:808	crude fucoidan from Sargassum Zhangii (ZF)	795:836	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	4	1	from	Zhangii	825:831	arg1	contents					772:779	the contents	768:779	the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF)	768:836	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	2	2	theme	chemical	329:336	arg1	composition					338:348	chemical composition	329:348	chemical composition	329:348	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	8	3	theme	polysaccharides	1286:1300	arg1	bands					1277:1281	the characteristic absorption bands	1247:1281	the characteristic absorption bands of polysaccharides and sulfate groups	1247:1319	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	8	4	theme	groups	1314:1319	arg1	bands					1277:1281	the characteristic absorption bands	1247:1281	the characteristic absorption bands of polysaccharides and sulfate groups	1247:1319	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	2	5	dep	nutrition	422:430	arg1	the					418:420	the	418:420	the	418:420	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	8	6	from	fucose	1338:1343	arg1	rich					1330:1333	rich	1330:1333	rich	1330:1333	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	8	7	theme	sulfate	1306:1312	arg1	groups					1314:1319	sulfate groups	1306:1319	sulfate groups	1306:1319	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	5	8	theme	bile	883:886	arg1	acid					888:891	bile acid	883:891	bile acid	883:891	Its ability of binding bile acid was better than other five crude fucoidans.
35741969	9	9	theme	bile	1374:1377	arg1	acid					1379:1382	bile acid	1374:1382	bile acid	1374:1382	Three fucoidans can bind to bile acid, and ZF2 showed the best binding capability.
35741969	2	10	dep	composition	338:348	arg1	the					325:327	the	325:327	the	325:327	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	3	11	theme	bile	678:681	arg1	acid					683:686	bile acid	678:686	bile acid	678:686	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	0	12	theme	Fucoidans	181:189	arg1	Acid					119:122	Binding Bile Acid	106:122	Binding Bile Acid	106:122	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	12	theme	Fucoidans	181:189	arg1	Composition					25:35	Nutritional and Chemical Composition	0:35	Nutritional and Chemical Composition of Sargassum zhangii	0:56	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	12	theme	Fucoidans	181:189	arg1	Activity					150:157	Cholesterol-Lowering Activity	129:157	Cholesterol-Lowering Activity	129:157	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	12	theme	Fucoidans	181:189	arg1	Characterization					88:103	the Physical and Chemical Characterization	62:103	the Physical and Chemical Characterization	62:103	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	10	13	dep	In	1429:1430	arg1	vitro					1432:1436	vitro	1432:1436	vitro	1432:1436	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	0	14	theme	Nutritional	0:10	arg1	Composition					25:35	Nutritional and Chemical Composition	0:35	Nutritional and Chemical Composition of Sargassum zhangii	0:56	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	15	from	Activity	150:157	arg1	Cells					168:172	HepG2 Cells	162:172	HepG2 Cells	162:172	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	16	theme	Bile	114:117	arg1	Acid					119:122	Binding Bile Acid	106:122	Binding Bile Acid	106:122	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	10	17	theme	cholesterol	1513:1523	arg1	content					1530:1536	intracellular total cholesterol (TC) content	1493:1536	intracellular total cholesterol (TC) content in HepG2 cells	1493:1551	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	3	18	dep	fractions	570:578	arg1	fractions					570:578	The purified fractions	557:578	The purified fractions (ZF1, ZF2, and ZF3)	557:598	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	3	18	dep	fractions	570:578	arg1	ZF2					586:588	ZF2	586:588	ZF2	586:588	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	3	18	dep	fractions	570:578	arg1	ZF3					595:597	ZF3	595:597	ZF3	595:597	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	3	18	dep	fractions	570:578	arg1	ZF1					581:583	ZF1	581:583	ZF1	581:583	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	6	19	theme	high	988:991	arg1	carbohydrate					993:1004	high carbohydrate	988:1004	high carbohydrate	988:1004	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	0	20	theme	Binding	106:112	arg1	Acid					119:122	Binding Bile Acid	106:122	Binding Bile Acid	106:122	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	8	21	from	rich	1330:1333	arg1	fucose					1338:1343	fucose	1338:1343	fucose	1338:1343	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	4	22	theme	sulfate	784:790	arg1	%					857:857	as high as13.63%	842:857	as high as13.63%	842:857	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	4	22	theme	sulfate	784:790	arg1	contents					772:779	the contents	768:779	the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF)	768:836	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	1	23	contain	has	267:269	arg2	range					278:282	a wide range	271:282	a wide range of biological activities	271:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	23	contain	has	267:269	arg1	polysaccharide					222:235	a marine sulfated polysaccharide	204:235	a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities	204:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	23	contain	has	267:269	arg1	Fucoidan					192:199	Fucoidan	192:199	Fucoidan	192:199	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	23	contain	has	267:269	arg1	rich					245:248	rich	245:248	rich	245:248	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	2	24	theme	binding	364:370	arg1	ability					372:378	bile acid binding ability	354:378	bile acid binding ability	354:378	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	7	25	theme	ZF1	1064:1066	arg1	%					1090:1090	3.29%	1086:1090	3.29%	1086:1090	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	7	25	theme	ZF1	1064:1066	arg1	content					1053:1059	The sulfate content	1041:1059	The sulfate content of ZF1, ZF2, and ZF3	1041:1080	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	3	26	from	lowering	704:711	arg1	cells					722:726	HepG2 cells	716:726	HepG2 cells	716:726	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	10	27	theme	HepG2	1541:1545	arg1	cells					1547:1551	HepG2 cells	1541:1551	HepG2 cells	1541:1551	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	6	28	theme	seaweed	975:981	arg1	kind					961:964	a kind	959:964	a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals	959:1038	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	6	28	theme	seaweed	975:981	arg1	zhangii					947:953	Sargassum zhangii	937:953	Sargassum zhangii	937:953	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	2	29	theme	acid	359:362	arg1	ability					372:378	bile acid binding ability	354:378	bile acid binding ability	354:378	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	1	30	from	rich	245:248	arg1	Sargassum					253:261	Sargassum	253:261	Sargassum	253:261	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	4	31	theme	crude	795:799	arg1	fucoidan					801:808	crude fucoidan	795:808	crude fucoidan from Sargassum Zhangii (ZF)	795:836	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	10	32	from	content	1530:1536	arg1	cells					1547:1551	HepG2 cells	1541:1551	HepG2 cells	1541:1551	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	8	33	theme	absorption	1266:1275	arg1	bands					1277:1281	the characteristic absorption bands	1247:1281	the characteristic absorption bands of polysaccharides and sulfate groups	1247:1319	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	2	34	theme	bile	354:357	arg1	ability					372:378	bile acid binding ability	354:378	bile acid binding ability	354:378	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	1	35	from	Sargassum	253:261	arg1	Fucoidan					192:199	Fucoidan	192:199	Fucoidan	192:199	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	35	from	Sargassum	253:261	arg1	polysaccharide					222:235	a marine sulfated polysaccharide	204:235	a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities	204:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	35	from	Sargassum	253:261	arg1	rich					245:248	rich	245:248	rich	245:248	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	6	36	from	kind	961:964	arg1	minerals					1031:1038	minerals	1031:1038	minerals	1031:1038	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	11	37	theme	best	1603:1606	arg1	ability					1608:1614	the best ability	1599:1614	the best ability to reduce TC	1599:1627	ZF2 showed the best ability to reduce TC.
35741969	6	38	from	minerals	1031:1038	arg1	kind					961:964	a kind	959:964	a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals	959:1038	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	6	38	from	minerals	1031:1038	arg1	rich					1023:1026	rich	1023:1026	rich	1023:1026	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	6	38	from	minerals	1031:1038	arg1	zhangii					947:953	Sargassum zhangii	937:953	Sargassum zhangii	937:953	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	2	39	theme	fucoidans	393:401	arg1	ability					372:378	bile acid binding ability	354:378	bile acid binding ability	354:378	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	2	39	theme	fucoidans	393:401	arg1	composition					338:348	chemical composition	329:348	chemical composition	329:348	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	0	40	theme	Chemical	16:23	arg1	Composition					25:35	Nutritional and Chemical Composition	0:35	Nutritional and Chemical Composition of Sargassum zhangii	0:56	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	1	41	theme	wide	273:276	arg1	range					278:282	a wide range	271:282	a wide range of biological activities	271:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	10	42	theme	intracellular	1493:1505	arg1	content					1530:1536	intracellular total cholesterol (TC) content	1493:1536	intracellular total cholesterol (TC) content in HepG2 cells	1493:1551	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	6	43	with	seaweed	975:981	arg1	carbohydrate					993:1004	high carbohydrate	988:1004	high carbohydrate	988:1004	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	6	43	with	seaweed	975:981	arg1	fat					1015:1017	low fat	1011:1017	low fat	1011:1017	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	7	44	theme	3.368	1190:1194	arg1	×					1196:1196	3.368 × 105	1190:1200	3.368 × 105	1190:1200	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	2	45	theme	crude	387:391	arg1	fucoidans					393:401	six crude fucoidans	383:401	six crude fucoidans	383:401	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	3	46	theme	cholesterol	692:702	arg1	lowering					704:711	cholesterol lowering	692:711	cholesterol lowering	692:711	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	0	47	theme	Cholesterol-Lowering	129:148	arg1	Activity					150:157	Cholesterol-Lowering Activity	129:157	Cholesterol-Lowering Activity	129:157	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	3	48	from	acid	683:686	arg1	cells					722:726	HepG2 cells	716:726	HepG2 cells	716:726	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	2	49	theme	chemical	436:443	arg1	composition					445:455	chemical composition	436:455	chemical composition	436:455	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	7	50	theme	sulfate	1045:1051	arg1	%					1090:1090	3.29%	1086:1090	3.29%	1086:1090	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	7	50	theme	sulfate	1045:1051	arg1	content					1053:1059	The sulfate content	1041:1059	The sulfate content of ZF1, ZF2, and ZF3	1041:1080	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	3	51	theme	HepG2	716:720	arg1	cells					722:726	HepG2 cells	716:726	HepG2 cells	716:726	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	1	52	theme	marine	206:211	arg1	polysaccharide					222:235	a marine sulfated polysaccharide	204:235	a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities	204:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	52	theme	marine	206:211	arg1	Fucoidan					192:199	Fucoidan	192:199	Fucoidan	192:199	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	52	theme	marine	206:211	arg1	rich					245:248	rich	245:248	rich	245:248	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	53	theme	biological	287:296	arg1	activities					298:307	biological activities	287:307	biological activities	287:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	4	54	from	contents	772:779	arg1	ZF					834:835	ZF	834:835	ZF	834:835	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	4	54	from	contents	772:779	arg1	fucoidan					801:808	crude fucoidan	795:808	crude fucoidan from Sargassum Zhangii (ZF)	795:836	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	4	54	from	contents	772:779	arg1	Zhangii					825:831	Sargassum Zhangii	815:831	Sargassum Zhangii (ZF)	815:836	The results showed that the contents of sulfate in crude fucoidan from Sargassum Zhangii (ZF) was as high as13.63%.
35741969	0	55	theme	zhangii	50:56	arg1	Acid					119:122	Binding Bile Acid	106:122	Binding Bile Acid	106:122	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	55	theme	zhangii	50:56	arg1	Composition					25:35	Nutritional and Chemical Composition	0:35	Nutritional and Chemical Composition of Sargassum zhangii	0:56	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	55	theme	zhangii	50:56	arg1	Activity					150:157	Cholesterol-Lowering Activity	129:157	Cholesterol-Lowering Activity	129:157	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	55	theme	zhangii	50:56	arg1	Characterization					88:103	the Physical and Chemical Characterization	62:103	the Physical and Chemical Characterization	62:103	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	1	56	theme	activities	298:307	arg1	range					278:282	a wide range	271:282	a wide range of biological activities	271:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	10	57	theme	total	1507:1511	arg1	TC					1526:1527	TC	1526:1527	TC	1526:1527	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	10	57	theme	total	1507:1511	arg1	cholesterol					1513:1523	total cholesterol	1507:1523	intracellular total cholesterol (TC) content in HepG2 cells	1493:1551	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	3	58	theme	physicochemical	617:631	arg1	characterization					633:648	physicochemical characterization	617:648	physicochemical characterization	617:648	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	3	59	theme	purified	561:568	arg1	fractions					570:578	The purified fractions	557:578	The purified fractions (ZF1, ZF2, and ZF3)	557:598	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	3	59	theme	purified	561:568	arg1	ZF2					586:588	ZF2	586:588	ZF2	586:588	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	3	59	theme	purified	561:568	arg1	ZF3					595:597	ZF3	595:597	ZF3	595:597	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	3	59	theme	purified	561:568	arg1	ZF1					581:583	ZF1	581:583	ZF1	581:583	The purified fractions (ZF1, ZF2, and ZF3) were analyzed by physicochemical characterization, and the ability of binding bile acid and cholesterol lowering in HepG2 cells were evaluated.
35741969	7	60	theme	ZF3	1078:1080	arg1	%					1090:1090	3.29%	1086:1090	3.29%	1086:1090	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	7	60	theme	ZF3	1078:1080	arg1	content					1053:1059	The sulfate content	1041:1059	The sulfate content of ZF1, ZF2, and ZF3	1041:1080	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	6	61	theme	rich	1023:1026	arg1	kind					961:964	a kind	959:964	a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals	959:1038	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	6	61	theme	rich	1023:1026	arg1	zhangii					947:953	Sargassum zhangii	937:953	Sargassum zhangii	937:953	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	5	62	theme	crude	920:924	arg1	fucoidans					926:934	other five crude fucoidans	909:934	other five crude fucoidans	909:934	Its ability of binding bile acid was better than other five crude fucoidans.
35741969	8	63	theme	characteristic	1251:1264	arg1	bands					1277:1281	the characteristic absorption bands	1247:1281	the characteristic absorption bands of polysaccharides and sulfate groups	1247:1319	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	6	64	from	rich	1023:1026	arg1	minerals					1031:1038	minerals	1031:1038	minerals	1031:1038	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	7	65	theme	ZF2	1069:1071	arg1	%					1090:1090	3.29%	1086:1090	3.29%	1086:1090	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	7	65	theme	ZF2	1069:1071	arg1	content					1053:1059	The sulfate content	1041:1059	The sulfate content of ZF1, ZF2, and ZF3	1041:1080	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	1	66	theme	sulfated	213:220	arg1	polysaccharide					222:235	a marine sulfated polysaccharide	204:235	a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities	204:307	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	66	theme	sulfated	213:220	arg1	Fucoidan					192:199	Fucoidan	192:199	Fucoidan	192:199	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	1	66	theme	sulfated	213:220	arg1	rich					245:248	rich	245:248	rich	245:248	Fucoidan is a marine sulfated polysaccharide that is rich in Sargassum and has a wide range of biological activities.
35741969	0	67	theme	HepG2	162:166	arg1	Cells					168:172	HepG2 Cells	162:172	HepG2 Cells	162:172	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	5	68	theme	other	909:913	arg1	fucoidans					926:934	other five crude fucoidans	909:934	other five crude fucoidans	909:934	Its ability of binding bile acid was better than other five crude fucoidans.
35741969	9	69	theme	binding	1409:1415	arg1	capability					1417:1426	the best binding capability	1400:1426	the best binding capability	1400:1426	Three fucoidans can bind to bile acid, and ZF2 showed the best binding capability.
35741969	6	70	theme	Sargassum	937:945	arg1	kind					961:964	a kind	959:964	a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals	959:1038	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	6	70	theme	Sargassum	937:945	arg1	zhangii					947:953	Sargassum zhangii	937:953	Sargassum zhangii	937:953	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	0	71	theme	Physical	66:73	arg1	Characterization					88:103	the Physical and Chemical Characterization	62:103	the Physical and Chemical Characterization	62:103	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	0	72	from	Characterization	88:103	arg1	Cells					168:172	HepG2 Cells	162:172	HepG2 Cells	162:172	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	9	73	theme	best	1404:1407	arg1	capability					1417:1426	the best binding capability	1400:1426	the best binding capability	1400:1426	Three fucoidans can bind to bile acid, and ZF2 showed the best binding capability.
35741969	7	74	theme	molecular	1134:1142	arg1	Mw					1152:1153	Mw	1152:1153	Mw	1152:1153	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	7	74	theme	molecular	1134:1142	arg1	weight					1144:1149	the molecular weight	1130:1149	the molecular weight (Mw)	1130:1154	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	7	74	theme	molecular	1134:1142	arg1	×					1166:1166	4.026 × 105	1160:1170	4.026 × 105	1160:1170	The sulfate content of ZF1, ZF2, and ZF3 was 3.29%, 19.39%, and 18.89% respectively, and the molecular weight (Mw) was 4.026 × 105, 2.893 × 105, and 3.368 × 105, respectively.
35741969	6	75	theme	low	1011:1013	arg1	fat					1015:1017	low fat	1011:1017	low fat	1011:1017	Sargassum zhangii was a kind of brown seaweed with high carbohydrate, and low fat and rich in minerals.
35741969	10	76	theme	In	1429:1430	arg1	experiments					1438:1448	In vitro experiments	1429:1448	In vitro experiments	1429:1448	In vitro experiments showed that ZF1, ZF2, and ZF3 could reduce intracellular total cholesterol (TC) content in HepG2 cells without affecting their viability.
35741969	2	77	theme	zhangii	470:476	arg1	nutrition					422:430	nutrition	422:430	nutrition	422:430	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	2	77	theme	zhangii	470:476	arg1	composition					445:455	chemical composition	436:455	chemical composition	436:455	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	0	78	from	Composition	25:35	arg1	Cells					168:172	HepG2 Cells	162:172	HepG2 Cells	162:172	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
35741969	2	79	from	zhangii	521:527	arg1	fucoidan					497:504	fucoidan	497:504	fucoidan from Sargassum zhangii	497:527	In this study, the chemical composition and bile acid binding ability of six crude fucoidans were compared, the nutrition and chemical composition of Sargassum zhangii were analyzed, and fucoidan from Sargassum zhangii was extracted and purified.
35741969	8	80	contain	contained	1237:1245	arg1	fucoidans					1223:1231	Three fucoidans	1217:1231	Three fucoidans	1217:1231	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	8	80	contain	contained	1237:1245	arg2	bands					1277:1281	the characteristic absorption bands	1247:1281	the characteristic absorption bands of polysaccharides and sulfate groups	1247:1319	Three fucoidans all contained the characteristic absorption bands of polysaccharides and sulfate groups and were rich in fucose.
35741969	0	81	from	Acid	119:122	arg1	Cells					168:172	HepG2 Cells	162:172	HepG2 Cells	162:172	Nutritional and Chemical Composition of Sargassum zhangii and the Physical and Chemical Characterization, Binding Bile Acid, and Cholesterol-Lowering Activity in HepG2 Cells of Its Fucoidans.
37230280	18	0	from	production	2479:2488	arg1	cells					2521:2525	BV2 cells	2517:2525	BV2 cells	2517:2525	In vitro, TSPJ suppressed LPS-induced production of inflammatory factors in BV2 cells via TLR4-MyD88-NF-κB signaling pathway.
37230280	4	1	theme	underlying	790:799	arg1	mechanisms					801:810	its possible underlying mechanisms	777:810	its possible underlying mechanisms	777:810	AIM OF THE STUDY To evaluate the therapeutic effect of TSPJ on EAE and explored its possible underlying mechanisms.
37230280	13	2	from	expression	1835:1844	arg1	cells					1880:1884	cells	1880:1884	cells	1880:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	3	3	from	effect	480:485	arg1	mice					575:578	experimental autoimmune encephalomyelitis (EAE) mice	527:578	experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS)	527:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	3	from	effect	480:485	arg1	TSPJ					518:521	TSPJ	518:521	TSPJ	518:521	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	15	4	theme	inflammatory	2107:2118	arg1	infiltration					2125:2136	inflammatory cell infiltration	2107:2136	inflammatory cell infiltration in the brain and spinal cord of EAE mice	2107:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	17	5	theme	IL-1β	2371:2375	arg1	levels					2344:2349	The levels	2340:2349	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum	2340:2403	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum also decreased post-TSPJ treatment.
37230280	13	6	from	p65	1862:1864	arg1	cells					1880:1884	cells	1880:1884	cells	1880:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	3	7	theme	Multiple	672:679	arg1	MS					692:693	MS	692:693	MS	692:693	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	7	theme	Multiple	672:679	arg1	sclerosis					681:689	Multiple sclerosis	672:689	Multiple sclerosis (MS)	672:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	1	8	from	weakness	315:322	arg1	China					327:331	China	327:331	China	327:331	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	17	9	theme	peripheral	2388:2397	arg1	serum					2399:2403	peripheral serum	2388:2403	peripheral serum	2388:2403	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum also decreased post-TSPJ treatment.
37230280	10	10	from	expression	1510:1519	arg1	CNS					1524:1526	CNS	1524:1526	CNS of the above indices	1524:1547	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	13	11	from	MyD88	1855:1859	arg1	cells					1880:1884	cells	1880:1884	cells	1880:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	10	12	theme	indices	1541:1547	arg1	CNS					1524:1526	CNS	1524:1526	CNS of the above indices	1524:1547	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	11	13	theme	Treg	1587:1590	arg1	Th1					1569:1571	Th1	1569:1571	Th1	1569:1571	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	11	13	theme	Treg	1587:1590	arg1	cells					1592:1596	Th17and Treg cells	1579:1596	Th17and Treg cells	1579:1596	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	3	14	theme	experimental	527:538	arg1	EAE					570:572	EAE	570:572	EAE	570:572	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	14	theme	experimental	527:538	arg1	encephalomyelitis					551:567	experimental autoimmune encephalomyelitis	527:567	experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS)	527:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	1	15	theme	Panax	216:220	arg1	RPJ					233:235	RPJ	233:235	RPJ	233:235	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	1	15	theme	Panax	216:220	arg1	medicine					260:267	a traditional herbal medicine	239:267	a traditional herbal medicine	239:267	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	1	15	theme	Panax	216:220	arg1	japonicus					222:230	Panax japonicus	216:230	Panax japonicus (RPJ)	216:236	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	15	16	theme	cell	2120:2123	arg1	infiltration					2125:2136	inflammatory cell infiltration	2107:2136	inflammatory cell infiltration in the brain and spinal cord of EAE mice	2107:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	8	17	from	levels	1264:1269	arg1	CNS					1297:1299	CNS	1297:1299	CNS	1297:1299	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	8	17	from	levels	1264:1269	arg1	system					1290:1295	central nervous system	1274:1295	central nervous system(CNS)	1274:1300	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	12	18	theme	intestinal	1705:1714	arg1	flora					1716:1720	the intestinal flora	1701:1720	the intestinal flora of mice in each group	1701:1742	Furthermore, 16S rDNA sequencing was used to detect the intestinal flora of mice in each group.
37230280	22	19	theme	anti-neuroinflammation	2953:2974	arg1	property					2976:2983	Its anti-neuroinflammation property	2949:2983	Its anti-neuroinflammation property in EAE	2949:2990	Its anti-neuroinflammation property in EAE was related to modulating gut microbiota and inhibiting TLR4-MyD88-NF-κB signaling pathway.
37230280	8	20	theme	nervous	1282:1288	arg1	CNS					1297:1299	CNS	1297:1299	CNS	1297:1299	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	8	20	theme	nervous	1282:1288	arg1	system					1290:1295	central nervous system	1274:1295	central nervous system(CNS)	1274:1300	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	21	21	theme	therapeutic	2921:2931	arg1	effects					2933:2939	therapeutic effects	2921:2939	therapeutic effects	2921:2939	CONCLUSION Our results demonstrated TSPJ had therapeutic effects on EAE.
37230280	10	22	theme	Quantitative	1437:1448	arg1	qRT-PCR					1477:1483	qRT-PCR	1477:1483	qRT-PCR	1477:1483	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	10	22	theme	Quantitative	1437:1448	arg1	PCR					1472:1474	Quantitative reverse transcription PCR	1437:1474	Quantitative reverse transcription PCR (qRT-PCR)	1437:1484	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	0	23	theme	gut	149:151	arg1	microbiota					153:162	gut microbiota	149:162	gut microbiota in mice	149:170	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	20	24	theme	correlation	2737:2747	arg1	analysis					2749:2756	Spearman's correlation analysis	2726:2756	Spearman's correlation analysis	2726:2756	Furthermore, Spearman's correlation analysis revealed that a relationship existed between statistically significantly altered genera and CNS inflammatory indices.
37230280	0	25	from	Japonicus	51:59	arg1	saponin					32:38	the total saponin	22:38	the total saponin from Panax Japonicus	22:59	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	0	25	from	Japonicus	51:59	arg1	effect					12:17	Therapeutic effect	0:17	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.	0:171	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	21	26	dep	demonstrated	2899:2910	arg1	had					2917:2919	had	2917:2919	demonstrated TSPJ had therapeutic effects on EAE	2899:2946	CONCLUSION Our results demonstrated TSPJ had therapeutic effects on EAE.
37230280	3	27	theme	encephalomyelitis	551:567	arg1	mice					575:578	experimental autoimmune encephalomyelitis (EAE) mice	527:578	experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS)	527:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	16	28	theme	mice	2334:2337	arg1	spleen					2320:2325	the spleen	2316:2325	the spleen of EAE mice	2316:2337	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	16	29	from	Th17/Treg	2279:2287	arg1	spleen					2320:2325	the spleen	2316:2325	the spleen of EAE mice	2316:2337	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	15	30	theme	infiltration	2125:2136	arg1	sheath					2079:2084	myelin sheath	2072:2084	myelin sheath	2072:2084	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	30	theme	infiltration	2125:2136	arg1	reduction					2094:2102	the reduction	2090:2102	the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice	2090:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	9	31	from	levels	1387:1392	arg1	CNS					1407:1409	CNS	1407:1409	CNS	1407:1409	The changes in IL-1β, IL-6, and TNF-α levels in serum and CNS were measured with ELISA.
37230280	9	31	from	levels	1387:1392	arg1	serum					1397:1401	serum	1397:1401	serum	1397:1401	The changes in IL-1β, IL-6, and TNF-α levels in serum and CNS were measured with ELISA.
37230280	15	32	from	sheath	2079:2084	arg1	brain					2145:2149	brain	2145:2149	brain	2145:2149	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	32	from	sheath	2079:2084	arg1	cord					2162:2165	spinal cord	2155:2165	spinal cord	2155:2165	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	6	33	dep	TSPJ	894:897	arg1	mg/kg					905:909	36.5 mg/kg	900:909	36.5 mg/kg	900:909	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	6	33	dep	TSPJ	894:897	arg1	postimmunization					996:1011	orally once daily up to 28 days postimmunization	964:1011	orally once daily up to 28 days postimmunization	964:1011	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	6	33	dep	TSPJ	894:897	arg1	mg/kg					915:919	73 mg/kg	912:919	73 mg/kg	912:919	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	7	34	theme	EAE-induced	1184:1194	arg1	changes					1209:1215	the EAE-induced pathological changes	1180:1215	the EAE-induced pathological changes in the brain and spinal cord	1180:1244	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	15	35	theme	mice	2174:2177	arg1	brain					2145:2149	brain	2145:2149	brain	2145:2149	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	35	theme	mice	2174:2177	arg1	cord					2162:2165	spinal cord	2155:2165	spinal cord	2155:2165	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	20	36	theme	inflammatory	2854:2865	arg1	indices					2867:2873	inflammatory indices	2854:2873	inflammatory indices	2854:2873	Furthermore, Spearman's correlation analysis revealed that a relationship existed between statistically significantly altered genera and CNS inflammatory indices.
37230280	8	37	theme	immunohistochemical	1319:1337	arg1	staining					1339:1346	immunohistochemical staining	1319:1346	immunohistochemical staining	1319:1346	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	16	38	from	ratios	2306:2311	arg1	spleen					2320:2325	the spleen	2316:2325	the spleen of EAE mice	2316:2337	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	0	39	theme	experimental	64:75	arg1	encephalomyelitis					88:104	experimental autoimmune encephalomyelitis	64:104	experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice	64:170	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	1	40	theme	Ming	342:345	arg1	dynasty					347:353	the Ming dynasty	338:353	the Ming dynasty	338:353	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	1	41	theme	ETHNOPHARMACOLOGICAL	173:192	arg1	Rhizomes					204:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes	173:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine,	173:268	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	16	42	theme	Th1/Th2	2293:2299	arg1	ratios					2306:2311	Th1/Th2 cell ratios	2293:2311	Th1/Th2 cell ratios	2293:2311	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	4	43	theme	STUDY	708:712	arg1	AIM					697:699	AIM	697:699	AIM OF THE STUDY	697:712	AIM OF THE STUDY To evaluate the therapeutic effect of TSPJ on EAE and explored its possible underlying mechanisms.
37230280	13	44	theme	BV2	1797:1799	arg1	microglia					1801:1809	-induced BV2 microglia	1788:1809	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	9	45	theme	TNF-α	1381:1385	arg1	levels					1387:1392	IL-1β, IL-6, and TNF-α levels	1364:1392	IL-1β, IL-6, and TNF-α levels in serum and CNS	1364:1409	The changes in IL-1β, IL-6, and TNF-α levels in serum and CNS were measured with ELISA.
37230280	7	46	from	changes	1209:1215	arg1	brain					1224:1228	brain	1224:1228	brain	1224:1228	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	7	46	from	changes	1209:1215	arg1	cord					1241:1244	spinal cord	1234:1244	spinal cord	1234:1244	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	17	47	theme	post-TSPJ	2420:2428	arg1	treatment					2430:2438	post-TSPJ treatment	2420:2438	post-TSPJ treatment	2420:2438	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum also decreased post-TSPJ treatment.
37230280	2	48	theme	main	384:387	arg1	components					399:408	the main bioactive components	380:408	the main bioactive components of RPJ	380:415	Triterpene saponins are the main bioactive components of RPJ.
37230280	2	48	theme	main	384:387	arg1	saponins					367:374	Triterpene saponins	356:374	Triterpene saponins	356:374	Triterpene saponins are the main bioactive components of RPJ.
37230280	0	49	theme	Therapeutic	0:10	arg1	effect					12:17	Therapeutic effect	0:17	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.	0:171	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	15	50	theme	Histological	2003:2014	arg1	examination					2016:2026	Histological examination	2003:2026	Histological examination	2003:2026	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	12	51	theme	rDNA	1666:1669	arg1	sequencing					1671:1680	16S rDNA sequencing	1662:1680	16S rDNA sequencing	1662:1680	Furthermore, 16S rDNA sequencing was used to detect the intestinal flora of mice in each group.
37230280	15	52	from	brain	2145:2149	arg1	sheath					2079:2084	myelin sheath	2072:2084	myelin sheath	2072:2084	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	52	from	brain	2145:2149	arg1	reduction					2094:2102	the reduction	2090:2102	the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice	2090:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	13	53	from	p-p65	1871:1875	arg1	cells					1880:1884	cells	1880:1884	cells	1880:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	1	54	used	used	274:277	arg2	Rhizomes					204:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes	173:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine,	173:268	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	6	55	theme	positive	946:953	arg1	acetate					937:943	prednisone acetate	926:943	prednisone acetate (positive control)	926:962	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	6	55	theme	positive	946:953	arg1	control					955:961	positive control	946:961	positive control	946:961	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	22	56	from	property	2976:2983	arg1	EAE					2988:2990	EAE	2988:2990	EAE	2988:2990	Its anti-neuroinflammation property in EAE was related to modulating gut microbiota and inhibiting TLR4-MyD88-NF-κB signaling pathway.
37230280	14	57	theme	neurological	1964:1975	arg1	impairment					1977:1986	neurological impairment	1964:1986	neurological impairment caused by EAE	1964:2000	RESULTS TSPJ treatment significantly alleviated neurological impairment caused by EAE.
37230280	2	58	theme	RPJ	413:415	arg1	components					399:408	the main bioactive components	380:408	the main bioactive components of RPJ	380:415	Triterpene saponins are the main bioactive components of RPJ.
37230280	2	58	theme	RPJ	413:415	arg1	saponins					367:374	Triterpene saponins	356:374	Triterpene saponins	356:374	Triterpene saponins are the main bioactive components of RPJ.
37230280	0	59	theme	total	26:30	arg1	saponin					32:38	the total saponin	22:38	the total saponin from Panax Japonicus	22:59	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	1	60	theme	physical	306:313	arg1	weakness					315:322	physical weakness	306:322	physical weakness	306:322	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	19	61	theme	microbiota	2635:2644	arg1	composition					2616:2626	the composition	2612:2626	the composition of gut microbiota	2612:2644	More importantly, TSPJ interventions altered the composition of gut microbiota and restored the ratio of Firmicutes to Bacteroidetes in EAE mice.
37230280	13	62	theme	Western	1902:1908	arg1	blot					1910:1913	Western blot	1902:1913	Western blot	1902:1913	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	3	63	theme	oligodendrocyte	598:612	arg1	glycoprotein					614:625	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	63	theme	oligodendrocyte	598:612	arg1	model					663:667	a commonly used animal model	640:667	a commonly used animal model of Multiple sclerosis (MS)	640:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	63	theme	oligodendrocyte	598:612	arg1	MOG					628:630	MOG	628:630	MOG	628:630	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	6	64	dep	days	991:994	arg1	up					982:983	up	982:983	up	982:983	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	13	65	theme	lipopolysaccharides	1763:1781	arg1	cells					1811:1815	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	21	66	dep	CONCLUSION	2876:2885	arg1	demonstrated					2899:2910	demonstrated	2899:2910	demonstrated TSPJ had therapeutic effects on EAE	2899:2946	CONCLUSION Our results demonstrated TSPJ had therapeutic effects on EAE.
37230280	13	67	from	cells	1880:1884	arg1	expression					1835:1844	the expression	1831:1844	the expression of TLR4, MyD88, p65, and p-p65 in cells	1831:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	18	68	theme	LPS-induced	2467:2477	arg1	production					2479:2488	LPS-induced production	2467:2488	LPS-induced production of inflammatory factors in BV2 cells	2467:2525	In vitro, TSPJ suppressed LPS-induced production of inflammatory factors in BV2 cells via TLR4-MyD88-NF-κB signaling pathway.
37230280	10	69	theme	mRNA	1505:1508	arg1	expression					1510:1519	access mRNA expression	1498:1519	access mRNA expression in CNS of the above indices	1498:1547	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	18	70	theme	TLR4-MyD88-NF-κB	2531:2546	arg1	pathway					2558:2564	TLR4-MyD88-NF-κB signaling pathway	2531:2564	TLR4-MyD88-NF-κB signaling pathway	2531:2564	In vitro, TSPJ suppressed LPS-induced production of inflammatory factors in BV2 cells via TLR4-MyD88-NF-κB signaling pathway.
37230280	22	71	theme	signaling	3065:3073	arg1	pathway					3075:3081	TLR4-MyD88-NF-κB signaling pathway	3048:3081	TLR4-MyD88-NF-κB signaling pathway	3048:3081	Its anti-neuroinflammation property in EAE was related to modulating gut microbiota and inhibiting TLR4-MyD88-NF-κB signaling pathway.
37230280	7	72	theme	electron	1125:1132	arg1	TEM					1146:1148	TEM	1146:1148	TEM	1146:1148	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	7	72	theme	electron	1125:1132	arg1	microscopy					1134:1143	transmission electron microscopy	1112:1143	transmission electron microscopy (TEM)	1112:1149	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	8	73	theme	central	1274:1280	arg1	CNS					1297:1299	CNS	1297:1299	CNS	1297:1299	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	8	73	theme	central	1274:1280	arg1	system					1290:1295	central nervous system	1274:1295	central nervous system(CNS)	1274:1300	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	18	74	theme	BV2	2517:2519	arg1	cells					2521:2525	BV2 cells	2517:2525	BV2 cells	2517:2525	In vitro, TSPJ suppressed LPS-induced production of inflammatory factors in BV2 cells via TLR4-MyD88-NF-κB signaling pathway.
37230280	10	75	theme	above	1535:1539	arg1	indices					1541:1547	the above indices	1531:1547	the above indices	1531:1547	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	1	76	theme	traditional	241:251	arg1	japonicus					222:230	Panax japonicus	216:230	Panax japonicus (RPJ)	216:236	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	1	76	theme	traditional	241:251	arg1	medicine					260:267	a traditional herbal medicine	239:267	a traditional herbal medicine	239:267	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	3	77	theme	animal	656:661	arg1	glycoprotein					614:625	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	77	theme	animal	656:661	arg1	model					663:667	a commonly used animal model	640:667	a commonly used animal model of Multiple sclerosis (MS)	640:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	18	78	theme	factors	2506:2512	arg1	production					2479:2488	LPS-induced production	2467:2488	LPS-induced production of inflammatory factors in BV2 cells	2467:2525	In vitro, TSPJ suppressed LPS-induced production of inflammatory factors in BV2 cells via TLR4-MyD88-NF-κB signaling pathway.
37230280	2	79	theme	Triterpene	356:365	arg1	components					399:408	the main bioactive components	380:408	the main bioactive components of RPJ	380:415	Triterpene saponins are the main bioactive components of RPJ.
37230280	2	79	theme	Triterpene	356:365	arg1	saponins					367:374	Triterpene saponins	356:374	Triterpene saponins	356:374	Triterpene saponins are the main bioactive components of RPJ.
37230280	15	80	theme	protective	2042:2051	arg1	effects					2053:2059	the protective effects	2038:2059	the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice	2038:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	18	81	theme	signaling	2548:2556	arg1	pathway					2558:2564	TLR4-MyD88-NF-κB signaling pathway	2531:2564	TLR4-MyD88-NF-κB signaling pathway	2531:2564	In vitro, TSPJ suppressed LPS-induced production of inflammatory factors in BV2 cells via TLR4-MyD88-NF-κB signaling pathway.
37230280	4	82	theme	possible	781:788	arg1	mechanisms					801:810	its possible underlying mechanisms	777:810	its possible underlying mechanisms	777:810	AIM OF THE STUDY To evaluate the therapeutic effect of TSPJ on EAE and explored its possible underlying mechanisms.
37230280	3	83	gly	glycoprotein	614:625	arg1	glycoprotein					614:625	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	83	gly	glycoprotein	614:625	arg1	model					663:667	a commonly used animal model	640:667	a commonly used animal model of Multiple sclerosis (MS)	640:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	83	gly	glycoprotein	614:625	arg1	MOG					628:630	MOG	628:630	MOG	628:630	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	13	84	theme	p-p65	1871:1875	arg1	expression					1835:1844	the expression	1831:1844	the expression of TLR4, MyD88, p65, and p-p65 in cells	1831:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	9	85	from	changes	1353:1359	arg1	levels					1387:1392	IL-1β, IL-6, and TNF-α levels	1364:1392	IL-1β, IL-6, and TNF-α levels in serum and CNS	1364:1409	The changes in IL-1β, IL-6, and TNF-α levels in serum and CNS were measured with ELISA.
37230280	9	86	theme	IL-1β	1364:1368	arg1	levels					1387:1392	IL-1β, IL-6, and TNF-α levels	1364:1392	IL-1β, IL-6, and TNF-α levels in serum and CNS	1364:1409	The changes in IL-1β, IL-6, and TNF-α levels in serum and CNS were measured with ELISA.
37230280	15	87	from	infiltration	2125:2136	arg1	brain					2145:2149	brain	2145:2149	brain	2145:2149	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	87	from	infiltration	2125:2136	arg1	cord					2162:2165	spinal cord	2155:2165	spinal cord	2155:2165	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	3	88	theme	sclerosis	681:689	arg1	glycoprotein					614:625	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	88	theme	sclerosis	681:689	arg1	model					663:667	a commonly used animal model	640:667	a commonly used animal model of Multiple sclerosis (MS)	640:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	1	89	from	arthritis	292:300	arg1	China					327:331	China	327:331	China	327:331	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	3	90	theme	saponin	500:506	arg1	effect					480:485	the therapeutic effect	464:485	the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS)	464:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	1	91	theme	RELEVANCE	194:202	arg1	Rhizomes					204:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes	173:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine,	173:268	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	7	92	theme	Fast	1091:1094	arg1	LFB					1102:1104	LFB	1102:1104	LFB	1102:1104	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	7	92	theme	Fast	1091:1094	arg1	Luxol					1085:1089	Luxol Fast Blue	1085:1099	Luxol Fast Blue (LFB)	1085:1105	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	12	93	used	used	1686:1689	arg2	sequencing					1671:1680	16S rDNA sequencing	1662:1680	16S rDNA sequencing	1662:1680	Furthermore, 16S rDNA sequencing was used to detect the intestinal flora of mice in each group.
37230280	22	94	theme	TLR4-MyD88-NF-κB	3048:3063	arg1	pathway					3075:3081	TLR4-MyD88-NF-κB signaling pathway	3048:3081	TLR4-MyD88-NF-κB signaling pathway	3048:3081	Its anti-neuroinflammation property in EAE was related to modulating gut microbiota and inhibiting TLR4-MyD88-NF-κB signaling pathway.
37230280	12	95	theme	mice	1725:1728	arg1	flora					1716:1720	the intestinal flora	1701:1720	the intestinal flora of mice in each group	1701:1742	Furthermore, 16S rDNA sequencing was used to detect the intestinal flora of mice in each group.
37230280	19	96	theme	TSPJ	2585:2588	arg1	interventions					2590:2602	TSPJ interventions	2585:2602	TSPJ interventions	2585:2602	More importantly, TSPJ interventions altered the composition of gut microbiota and restored the ratio of Firmicutes to Bacteroidetes in EAE mice.
37230280	8	97	theme	IL-17a	1247:1252	arg1	levels					1264:1269	IL-17a and Foxp3 levels	1247:1269	IL-17a and Foxp3 levels in central nervous system(CNS)	1247:1300	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	1	98	theme	japonicus	222:230	arg1	Rhizomes					204:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes	173:211	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine,	173:268	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	6	99	theme	neurological	1024:1035	arg1	deficit					1037:1043	their neurological deficit	1018:1043	their neurological deficit	1018:1043	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	10	100	theme	reverse	1450:1456	arg1	qRT-PCR					1477:1483	qRT-PCR	1477:1483	qRT-PCR	1477:1483	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	10	100	theme	reverse	1450:1456	arg1	PCR					1472:1474	Quantitative reverse transcription PCR	1437:1474	Quantitative reverse transcription PCR (qRT-PCR)	1437:1484	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	11	101	theme	Th17and	1579:1585	arg1	Th1					1569:1571	Th1	1569:1571	Th1	1569:1571	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	11	101	theme	Th17and	1579:1585	arg1	cells					1592:1596	Th17and Treg cells	1579:1596	Th17and Treg cells	1579:1596	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	13	102	theme	MyD88	1855:1859	arg1	expression					1835:1844	the expression	1831:1844	the expression of TLR4, MyD88, p65, and p-p65 in cells	1831:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	13	103	used	used	1822:1825	arg2	cells					1811:1815	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	16	104	theme	EAE	2330:2332	arg1	mice					2334:2337	EAE mice	2330:2337	EAE mice	2330:2337	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	15	105	from	effects	2053:2059	arg1	sheath					2079:2084	myelin sheath	2072:2084	myelin sheath	2072:2084	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	105	from	effects	2053:2059	arg1	reduction					2094:2102	the reduction	2090:2102	the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice	2090:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	7	106	theme	spinal	1234:1239	arg1	cord					1241:1244	spinal cord	1234:1244	spinal cord	1234:1244	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	13	107	theme	p65	1862:1864	arg1	expression					1835:1844	the expression	1831:1844	the expression of TLR4, MyD88, p65, and p-p65 in cells	1831:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	3	108	theme	autoimmune	540:549	arg1	EAE					570:572	EAE	570:572	EAE	570:572	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	108	theme	autoimmune	540:549	arg1	encephalomyelitis					551:567	experimental autoimmune encephalomyelitis	527:567	experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS)	527:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	15	109	dep	brain	2145:2149	arg1	the					2141:2143	the	2141:2143	the	2141:2143	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	17	110	theme	IL-6	2361:2364	arg1	levels					2344:2349	The levels	2340:2349	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum	2340:2403	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum also decreased post-TSPJ treatment.
37230280	16	111	theme	mRNA	2248:2251	arg1	levels					2253:2258	protein and mRNA levels	2236:2258	levels	2253:2258	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	3	112	from	TSPJ	518:521	arg1	saponin					500:506	the total saponin	490:506	the total saponin from RPJ (TSPJ)	490:522	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	112	from	TSPJ	518:521	arg1	effect					480:485	the therapeutic effect	464:485	the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS)	464:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	23	113	theme	MS	3164:3165	arg1	treatment					3151:3159	the treatment	3147:3159	the treatment of MS	3147:3165	Our study indicated that TSPJ may be a potential candidate for the treatment of MS.
37230280	4	114	from	effect	742:747	arg1	EAE					760:762	EAE	760:762	EAE	760:762	AIM OF THE STUDY To evaluate the therapeutic effect of TSPJ on EAE and explored its possible underlying mechanisms.
37230280	6	115	theme	prednisone	926:935	arg1	acetate					937:943	prednisone acetate	926:943	prednisone acetate (positive control)	926:962	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	6	115	theme	prednisone	926:935	arg1	control					955:961	positive control	946:961	positive control	946:961	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	11	116	from	percentages	1554:1564	arg1	spleen					1601:1606	spleen	1601:1606	spleen	1601:1606	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	7	117	theme	Blue	1096:1099	arg1	LFB					1102:1104	LFB	1102:1104	LFB	1102:1104	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	7	117	theme	Blue	1096:1099	arg1	Luxol					1085:1089	Luxol Fast Blue	1085:1099	Luxol Fast Blue (LFB)	1085:1105	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	13	118	theme	TLR4	1849:1852	arg1	expression					1835:1844	the expression	1831:1844	the expression of TLR4, MyD88, p65, and p-p65 in cells	1831:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	5	119	theme	MATERIALS	813:821	arg1	EAE					835:837	MATERIALS AND METHODS EAE	813:837	MATERIALS AND METHODS EAE	813:837	MATERIALS AND METHODS EAE was induced by MOG 35-55.
37230280	7	120	theme	pathological	1196:1207	arg1	changes					1209:1215	the EAE-induced pathological changes	1180:1215	the EAE-induced pathological changes in the brain and spinal cord	1180:1244	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	0	121	theme	autoimmune	77:86	arg1	encephalomyelitis					88:104	experimental autoimmune encephalomyelitis	64:104	experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice	64:170	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	15	122	theme	EAE	2170:2172	arg1	mice					2174:2177	EAE mice	2170:2177	EAE mice	2170:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	5	123	theme	METHODS	827:833	arg1	EAE					835:837	MATERIALS AND METHODS EAE	813:837	MATERIALS AND METHODS EAE	813:837	MATERIALS AND METHODS EAE was induced by MOG 35-55.
37230280	15	124	from	reduction	2094:2102	arg1	brain					2145:2149	brain	2145:2149	brain	2145:2149	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	124	from	reduction	2094:2102	arg1	cord					2162:2165	spinal cord	2155:2165	spinal cord	2155:2165	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	0	125	from	effect	12:17	arg1	Japonicus					51:59	Panax Japonicus	45:59	Panax Japonicus	45:59	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	0	125	from	effect	12:17	arg1	encephalomyelitis					88:104	experimental autoimmune encephalomyelitis	64:104	experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice	64:170	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	16	126	from	CNS	2263:2265	arg1	spleen					2320:2325	the spleen	2316:2325	the spleen of EAE mice	2316:2337	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	23	127	theme	potential	3123:3131	arg1	candidate					3133:3141	a potential candidate	3121:3141	a potential candidate for the treatment of MS	3121:3165	Our study indicated that TSPJ may be a potential candidate for the treatment of MS.
37230280	23	127	theme	potential	3123:3131	arg1	TSPJ					3109:3112	TSPJ	3109:3112	TSPJ	3109:3112	Our study indicated that TSPJ may be a potential candidate for the treatment of MS.
37230280	9	128	theme	IL-6	1371:1374	arg1	levels					1387:1392	IL-1β, IL-6, and TNF-α levels	1364:1392	IL-1β, IL-6, and TNF-α levels in serum and CNS	1364:1409	The changes in IL-1β, IL-6, and TNF-α levels in serum and CNS were measured with ELISA.
37230280	16	129	theme	cell	2301:2304	arg1	ratios					2306:2311	Th1/Th2 cell ratios	2293:2311	Th1/Th2 cell ratios	2293:2311	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	11	130	theme	Flow	1627:1630	arg1	Cytometry					1632:1640	Flow Cytometry	1627:1640	Flow Cytometry (FCM)	1627:1646	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	11	130	theme	Flow	1627:1630	arg1	FCM					1643:1645	FCM	1643:1645	FCM	1643:1645	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	13	131	theme	-induced	1788:1795	arg1	microglia					1801:1809	-induced BV2 microglia	1788:1809	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	12	132	theme	16S	1662:1664	arg1	sequencing					1671:1680	16S rDNA sequencing	1662:1680	16S rDNA sequencing	1662:1680	Furthermore, 16S rDNA sequencing was used to detect the intestinal flora of mice in each group.
37230280	10	133	used	used	1490:1493	arg2	qRT-PCR					1477:1483	qRT-PCR	1477:1483	qRT-PCR	1477:1483	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	10	133	used	used	1490:1493	arg2	PCR					1472:1474	Quantitative reverse transcription PCR	1437:1474	Quantitative reverse transcription PCR (qRT-PCR)	1437:1484	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	6	134	theme	daily	976:980	arg1	postimmunization					996:1011	orally once daily up to 28 days postimmunization	964:1011	orally once daily up to 28 days postimmunization	964:1011	Mice were administrated with TSPJ (36.5 mg/kg, 73 mg/kg) and prednisone acetate (positive control) orally once daily up to 28 days postimmunization, and their neurological deficit was scored.
37230280	11	135	theme	Th1	1569:1571	arg1	percentages					1554:1564	The percentages	1550:1564	The percentages of Th1, Th2, Th17and Treg cells in spleen	1550:1606	The percentages of Th1, Th2, Th17and Treg cells in spleen were determined by Flow Cytometry (FCM).
37230280	19	136	theme	gut	2631:2633	arg1	microbiota					2635:2644	gut microbiota	2631:2644	gut microbiota	2631:2644	More importantly, TSPJ interventions altered the composition of gut microbiota and restored the ratio of Firmicutes to Bacteroidetes in EAE mice.
37230280	20	137	theme	altered	2831:2837	arg1	genera					2839:2844	statistically significantly altered genera	2803:2844	statistically significantly altered genera	2803:2844	Furthermore, Spearman's correlation analysis revealed that a relationship existed between statistically significantly altered genera and CNS inflammatory indices.
37230280	2	138	theme	bioactive	389:397	arg1	components					399:408	the main bioactive components	380:408	the main bioactive components of RPJ	380:415	Triterpene saponins are the main bioactive components of RPJ.
37230280	2	138	theme	bioactive	389:397	arg1	saponins					367:374	Triterpene saponins	356:374	Triterpene saponins	356:374	Triterpene saponins are the main bioactive components of RPJ.
37230280	13	139	theme	microglia	1801:1809	arg1	cells					1811:1815	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	lipopolysaccharides (LPS)-induced BV2 microglia cells	1763:1815	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	19	140	theme	Firmicutes	2672:2681	arg1	ratio					2663:2667	the ratio	2659:2667	the ratio of Firmicutes to Bacteroidetes in EAE mice	2659:2710	More importantly, TSPJ interventions altered the composition of gut microbiota and restored the ratio of Firmicutes to Bacteroidetes in EAE mice.
37230280	10	141	theme	transcription	1458:1470	arg1	qRT-PCR					1477:1483	qRT-PCR	1477:1483	qRT-PCR	1477:1483	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	10	141	theme	transcription	1458:1470	arg1	PCR					1472:1474	Quantitative reverse transcription PCR	1437:1474	Quantitative reverse transcription PCR (qRT-PCR)	1437:1484	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	4	142	theme	therapeutic	730:740	arg1	effect					742:747	the therapeutic effect	726:747	the therapeutic effect of TSPJ on EAE	726:762	AIM OF THE STUDY To evaluate the therapeutic effect of TSPJ on EAE and explored its possible underlying mechanisms.
37230280	22	143	theme	gut	3018:3020	arg1	microbiota					3022:3031	gut microbiota	3018:3031	gut microbiota	3018:3031	Its anti-neuroinflammation property in EAE was related to modulating gut microbiota and inhibiting TLR4-MyD88-NF-κB signaling pathway.
37230280	20	144	dep	genera	2839:2844	arg1	indices					2867:2873	inflammatory indices	2854:2873	inflammatory indices	2854:2873	Furthermore, Spearman's correlation analysis revealed that a relationship existed between statistically significantly altered genera and CNS inflammatory indices.
37230280	0	145	theme	saponin	32:38	arg1	effect					12:17	Therapeutic effect	0:17	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.	0:171	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	19	146	from	Bacteroidetes	2686:2698	arg1	mice					2707:2710	EAE mice	2703:2710	EAE mice	2703:2710	More importantly, TSPJ interventions altered the composition of gut microbiota and restored the ratio of Firmicutes to Bacteroidetes in EAE mice.
37230280	16	147	theme	IL-17a/Foxp3	2220:2231	arg1	ratio					2211:2215	the ratio	2207:2215	the ratio of IL-17a/Foxp3	2207:2231	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	15	148	from	cord	2162:2165	arg1	sheath					2079:2084	myelin sheath	2072:2084	myelin sheath	2072:2084	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	15	148	from	cord	2162:2165	arg1	reduction					2094:2102	the reduction	2090:2102	the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice	2090:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	19	149	theme	EAE	2703:2705	arg1	mice					2707:2710	EAE mice	2703:2710	EAE mice	2703:2710	More importantly, TSPJ interventions altered the composition of gut microbiota and restored the ratio of Firmicutes to Bacteroidetes in EAE mice.
37230280	3	150	theme	myelin	591:596	arg1	glycoprotein					614:625	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	150	theme	myelin	591:596	arg1	model					663:667	a commonly used animal model	640:667	a commonly used animal model of Multiple sclerosis (MS)	640:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	150	theme	myelin	591:596	arg1	MOG					628:630	MOG	628:630	MOG	628:630	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	18	151	theme	inflammatory	2493:2504	arg1	factors					2506:2512	inflammatory factors	2493:2512	inflammatory factors	2493:2512	In vitro, TSPJ suppressed LPS-induced production of inflammatory factors in BV2 cells via TLR4-MyD88-NF-κB signaling pathway.
37230280	3	152	theme	first	440:444	arg1	time					446:449	the first time	436:449	the first time	436:449	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	0	153	theme	Panax	45:49	arg1	Japonicus					51:59	Panax Japonicus	45:59	Panax Japonicus	45:59	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	16	154	theme	protein	2236:2242	arg1	levels					2253:2258	protein and mRNA levels	2236:2258	levels	2253:2258	TSPJ notably downregulated the ratio of IL-17a/Foxp3 at protein and mRNA levels in CNS, as well as Th17/Treg and Th1/Th2 cell ratios in the spleen of EAE mice.
37230280	15	155	theme	spinal	2155:2160	arg1	cord					2162:2165	spinal cord	2155:2165	spinal cord	2155:2165	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	17	156	theme	TNF-α	2354:2358	arg1	levels					2344:2349	The levels	2340:2349	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum	2340:2403	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum also decreased post-TSPJ treatment.
37230280	4	157	theme	TSPJ	752:755	arg1	effect					742:747	the therapeutic effect	726:747	the therapeutic effect of TSPJ on EAE	726:762	AIM OF THE STUDY To evaluate the therapeutic effect of TSPJ on EAE and explored its possible underlying mechanisms.
37230280	3	158	theme	therapeutic	468:478	arg1	effect					480:485	the therapeutic effect	464:485	the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS)	464:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	12	159	from	flora	1716:1720	arg1	group					1738:1742	each group	1733:1742	each group	1733:1742	Furthermore, 16S rDNA sequencing was used to detect the intestinal flora of mice in each group.
37230280	7	160	theme	transmission	1112:1123	arg1	TEM					1146:1148	TEM	1146:1148	TEM	1146:1148	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	7	160	theme	transmission	1112:1123	arg1	microscopy					1134:1143	transmission electron microscopy	1112:1143	transmission electron microscopy (TEM)	1112:1149	Hematoxylin and Eosin (HE), Luxol Fast Blue (LFB), and transmission electron microscopy (TEM) were carried out to evaluate the EAE-induced pathological changes in the brain and spinal cord.
37230280	0	161	from	microbiota	153:162	arg1	mice					167:170	mice	167:170	mice	167:170	Therapeutic effect of the total saponin from Panax Japonicus on experimental autoimmune encephalomyelitis by attenuating inflammation and regulating gut microbiota in mice.
37230280	8	162	theme	Foxp3	1258:1262	arg1	levels					1264:1269	IL-17a and Foxp3 levels	1247:1269	IL-17a and Foxp3 levels in central nervous system(CNS)	1247:1300	IL-17a and Foxp3 levels in central nervous system(CNS)were evaluated by immunohistochemical staining.
37230280	10	163	theme	access	1498:1503	arg1	expression					1510:1519	access mRNA expression	1498:1519	access mRNA expression in CNS of the above indices	1498:1547	Quantitative reverse transcription PCR (qRT-PCR) was used to access mRNA expression in CNS of the above indices.
37230280	15	164	theme	myelin	2072:2077	arg1	sheath					2079:2084	myelin sheath	2072:2084	myelin sheath	2072:2084	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	17	165	from	levels	2344:2349	arg1	CNS					2380:2382	CNS	2380:2382	CNS	2380:2382	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum also decreased post-TSPJ treatment.
37230280	17	165	from	levels	2344:2349	arg1	serum					2399:2403	peripheral serum	2388:2403	peripheral serum	2388:2403	The levels of TNF-α, IL-6, and IL-1β in CNS and peripheral serum also decreased post-TSPJ treatment.
37230280	13	166	from	TLR4	1849:1852	arg1	cells					1880:1884	cells	1880:1884	cells	1880:1884	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	3	167	theme	used	651:654	arg1	glycoprotein					614:625	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	myelin oligodendrocyte glycoprotein (MOG) 35-55	591:637	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	3	167	theme	used	651:654	arg1	model					663:667	a commonly used animal model	640:667	a commonly used animal model of Multiple sclerosis (MS)	640:694	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	15	168	theme	TSPJ	2064:2067	arg1	effects					2053:2059	the protective effects	2038:2059	the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice	2038:2177	Histological examination confirmed the protective effects of TSPJ on myelin sheath and the reduction of inflammatory cell infiltration in the brain and spinal cord of EAE mice.
37230280	13	169	theme	vitro	1748:1752	arg1	studies					1754:1760	vitro studies	1748:1760	vitro studies	1748:1760	In vitro studies, lipopolysaccharides (LPS)-induced BV2 microglia cells were used and the expression of TLR4, MyD88, p65, and p-p65 in cells was detected by Western blot.
37230280	3	170	theme	total	494:498	arg1	saponin					500:506	the total saponin	490:506	the total saponin from RPJ (TSPJ)	490:522	In this work, for the first time, we evaluate the therapeutic effect of the total saponin from RPJ (TSPJ) on experimental autoimmune encephalomyelitis (EAE) mice induced by myelin oligodendrocyte glycoprotein (MOG) 35-55, a commonly used animal model of Multiple sclerosis (MS).
37230280	1	171	theme	herbal	253:258	arg1	japonicus					222:230	Panax japonicus	216:230	Panax japonicus (RPJ)	216:236	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
37230280	1	171	theme	herbal	253:258	arg1	medicine					260:267	a traditional herbal medicine	239:267	a traditional herbal medicine	239:267	ETHNOPHARMACOLOGICAL RELEVANCE Rhizomes of Panax japonicus (RPJ), a traditional herbal medicine, was used for treating arthritis and physical weakness in China from the Ming dynasty.
36015417	2	0	theme	hepatocyte	594:603	arg1	membranes					605:613	the hepatocyte membranes	590:613	the hepatocyte membranes (viz	590:618	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	8	1	located	found	1524:1528	arg1	FME					1541:1543	the FME	1537:1543	the FME	1537:1543	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	1	located	found	1524:1528	arg2	μg/g					1517:1520	536.11 μg/g	1510:1520	536.11 μg/g	1510:1520	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	1	located	found	1524:1528	arg2	acid					1504:1507	quinic acid	1497:1507	quinic acid (536.11 μg/g)	1497:1521	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	1	2	theme	flower	271:276	arg1	analysis					241:248	the chemical composition analysis	216:248	the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	216:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	10	3	theme	clinical	2112:2119	arg1	therapy					2121:2127	clinical therapy	2112:2127	clinical therapy for the prevention of toxin-induced liver diseases	2112:2178	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	3	4	theme	consequent	703:712	arg1	break					728:732	consequent double-strand break	703:732	consequent double-strand break (namely genotoxicity)	703:754	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	1	5	theme	leaf	279:282	arg1	analysis					241:248	the chemical composition analysis	216:248	the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	216:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	10	6	theme	toxin-induced	2151:2163	arg1	diseases					2171:2178	toxin-induced liver diseases	2151:2178	toxin-induced liver diseases	2151:2178	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	9	7	theme	rat	1991:1993	arg1	break					2013:2017	the rat DNA double-strand break	1987:2017	the rat DNA double-strand break	1987:2017	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	5	8	theme	liver	1159:1163	arg1	status					1171:1176	the liver redox status	1155:1176	the liver redox status	1155:1176	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	9	9	theme	double-strand	1999:2011	arg1	break					2013:2017	the rat DNA double-strand break	1987:2017	the rat DNA double-strand break	1987:2017	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	2	10	theme	trichloromethylperoxy	524:544	arg1	radicals					546:553	trichloromethyl and trichloromethylperoxy radicals	504:553	trichloromethyl and trichloromethylperoxy radicals	504:553	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	3	11	theme	break	728:732	arg1	hepatotoxicity					621:634	hepatotoxicity)	621:635	hepatotoxicity)	621:635	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	3	11	theme	break	728:732	arg1	formation					664:672	the subsequent formation	649:672	the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity)	649:754	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	2	12	theme	trichloromethyl	504:518	arg1	radicals					546:553	trichloromethyl and trichloromethylperoxy radicals	504:553	trichloromethyl and trichloromethylperoxy radicals	504:553	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	5	13	theme	redox	1165:1169	arg1	status					1171:1176	the liver redox status	1155:1176	the liver redox status	1155:1176	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	1	14	theme	hepatoprotective	359:374	arg1	features					394:401	their hepatoprotective and antigenotoxic features	353:401	Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	253:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	8	15	theme	chlorogenic	1644:1654	arg1	acid					1656:1659	chlorogenic acid	1644:1659	chlorogenic acid (1408.43 μg/g)	1644:1674	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	15	theme	chlorogenic	1644:1654	arg1	μg/g					1670:1673	1408.43 μg/g	1662:1673	1408.43 μg/g	1662:1673	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	1	16	theme	composition	229:239	arg1	analysis					241:248	the chemical composition analysis	216:248	the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	216:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	2	17	theme	lipid	564:568	arg1	peroxidation					570:581	lipid peroxidation	564:581	lipid peroxidation within the hepatocyte membranes (viz	564:618	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	10	18	from	extracts	2058:2065	arg1	concentrations					2080:2093	specified concentrations	2070:2093	specified concentrations	2070:2093	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	6	19	theme	liver	1269:1273	arg1	markers					1284:1290	liver toxicity markers	1269:1290	liver toxicity markers	1269:1290	Furthermore, the necrosis of hepatocytes was assessed by means of catalytic activities of liver toxicity markers.
36015417	1	20	theme	antigenotoxic	380:392	arg1	features					394:401	their hepatoprotective and antigenotoxic features	353:401	Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	253:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	4	21	theme	pharmacological	873:887	arg1	properties					889:898	the most notable pharmacological properties	856:898	the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight	856:961	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	0	22	theme	Molecular	158:166	arg1	Investigations					177:190	Molecular Modeling Investigations	158:190	In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations	95:190	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	1	23	dep	flower	271:276	arg1	SME					323:325	SME	323:325	SME	323:325	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	1	23	dep	flower	271:276	arg1	LME					318:320	LME	318:320	LME	318:320	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	1	23	dep	flower	271:276	arg1	FME					313:315	FME	313:315	FME	313:315	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	10	24	theme	examined	2038:2045	arg1	extracts					2058:2065	the examined N. cataria extracts	2034:2065	the examined N. cataria extracts in specified concentrations	2034:2093	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	3	25	dep	break	728:732	arg1	genotoxicity					742:753	genotoxicity	742:753	namely genotoxicity	735:753	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	4	26	theme	mg/kg	942:946	arg1	concentrations					907:920	the concentrations	903:920	the concentrations of 200, 100, and 50 mg/kg of body weight	903:961	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	8	27	theme	chlorogenic	1460:1470	arg1	acid					1472:1475	chlorogenic acid	1460:1475	chlorogenic acid (1647.32 μg/g)	1460:1490	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	27	theme	chlorogenic	1460:1470	arg1	μg/g					1486:1489	1647.32 μg/g	1478:1489	1647.32 μg/g	1478:1489	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	4	28	theme	notable	865:871	arg1	properties					889:898	the most notable pharmacological properties	856:898	the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight	856:961	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	2	29	with	intoxication	451:462	arg1	CCl4					469:472	CCl4	469:472	CCl4	469:472	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	10	30	theme	cataria	2050:2056	arg1	extracts					2058:2065	the examined N. cataria extracts	2034:2065	the examined N. cataria extracts in specified concentrations	2034:2093	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	5	31	theme	catalytic	1058:1066	arg1	activities					1068:1077	the catalytic activities	1054:1077	the catalytic activities of enzymes	1054:1088	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	2	32	theme	rat	441:443	arg1	intoxication					451:462	Wistar rat liver intoxication	434:462	Wistar rat liver intoxication with CCl4	434:472	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	9	33	theme	rat	1906:1908	arg1	enzyme					1969:1974	an enzyme	1966:1974	an enzyme catalyzing the rat DNA double-strand break	1966:2017	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	9	33	theme	rat	1906:1908	arg1	catalase					1910:1917	rat catalase	1906:1917	rat catalase	1906:1917	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	10	34	used	used	2104:2107	arg2	extracts					2058:2065	the examined N. cataria extracts	2034:2065	the examined N. cataria extracts in specified concentrations	2034:2093	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	0	35	theme	Chemical	0:7	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition of Various Nepeta cataria Plant Organs	0:58	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	5	36	theme	species	1135:1141	arg1	concentrations					1098:1111	the concentrations	1094:1111	the concentrations of reactive oxidative species, modulating the liver redox status	1094:1176	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	5	36	theme	species	1135:1141	arg1	activities					1068:1077	the catalytic activities	1054:1077	the catalytic activities of enzymes	1054:1088	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	8	37	theme	quinic	1497:1502	arg1	acid					1504:1507	quinic acid	1497:1507	quinic acid (536.11 μg/g)	1497:1521	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	37	theme	quinic	1497:1502	arg1	μg/g					1517:1520	536.11 μg/g	1510:1520	536.11 μg/g	1510:1520	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	1	38	theme	stem	289:292	arg1	extracts					303:310	stem methanol extracts	289:310	stem methanol extracts	289:310	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	4	39	theme	body	951:954	arg1	weight					956:961	body weight	951:961	body weight	951:961	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	0	40	theme	Nepeta	32:37	arg1	Organs					53:58	Various Nepeta cataria Plant Organs	24:58	Various Nepeta cataria Plant Organs	24:58	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	1	41	theme	extracts	303:310	arg1	analysis					241:248	the chemical composition analysis	216:248	the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	216:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	9	42	dep	extracts	1786:1793	arg1	elucidated					1838:1847	elucidated	1838:1847	was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break	1834:2017	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	8	43	theme	pharmacological	1378:1392	arg1	features					1403:1410	Distinct pharmacological property features	1369:1410	Distinct pharmacological property features	1369:1410	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	3	44	theme	adducts	691:697	arg1	hepatotoxicity					621:634	hepatotoxicity)	621:635	hepatotoxicity)	621:635	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	3	44	theme	adducts	691:697	arg1	formation					664:672	the subsequent formation	649:672	the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity)	649:754	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	0	45	theme	Plant	47:51	arg1	Organs					53:58	Various Nepeta cataria Plant Organs	24:58	Various Nepeta cataria Plant Organs	24:58	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	5	46	theme	reactive	1116:1123	arg1	species					1135:1141	reactive oxidative species	1116:1141	reactive oxidative species	1116:1141	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	3	47	theme	subsequent	653:662	arg1	formation					664:672	the subsequent formation	649:672	the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity)	649:754	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	1	48	theme	Nepeta	253:258	arg1	flower					271:276	Nepeta cataria L. flower	253:276	Nepeta cataria L. flower	253:276	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	3	49	theme	rDNA	686:689	arg1	adducts					691:697	aberrant rDNA adducts	677:697	aberrant rDNA adducts	677:697	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	6	50	theme	catalytic	1245:1253	arg1	activities					1255:1264	catalytic activities	1245:1264	catalytic activities of liver toxicity markers	1245:1290	Furthermore, the necrosis of hepatocytes was assessed by means of catalytic activities of liver toxicity markers.
36015417	8	51	theme	chlorogenic	1582:1592	arg1	acid					1594:1597	chlorogenic acid	1582:1597	chlorogenic acid (648.52 μg/g)	1582:1611	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	51	theme	chlorogenic	1582:1592	arg1	μg/g					1607:1610	648.52 μg/g	1600:1610	648.52 μg/g	1600:1610	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	1	52	theme	L.	268:269	arg1	flower					271:276	Nepeta cataria L. flower	253:276	Nepeta cataria L. flower	253:276	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	8	53	theme	rosmarinic	1546:1555	arg1	acid					1557:1560	rosmarinic acid	1546:1560	rosmarinic acid (1056.14 μg/g)	1546:1575	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	53	theme	rosmarinic	1546:1555	arg1	μg/g					1571:1574	1056.14 μg/g	1563:1574	1056.14 μg/g	1563:1574	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	4	54	theme	CCl4	843:846	arg1	injection					830:838	the injection	826:838	the injection of CCl4	826:846	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	3	55	theme	double-strand	714:726	arg1	break					728:732	consequent double-strand break	703:732	consequent double-strand break (namely genotoxicity)	703:754	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	4	56	theme	Wistar	807:812	arg1	rats					814:817	Wistar rats	807:817	Wistar rats	807:817	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	9	57	theme	DNA	1995:1997	arg1	break					2013:2017	the rat DNA double-strand break	1987:2017	the rat DNA double-strand break	1987:2017	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	6	58	theme	hepatocytes	1208:1218	arg1	necrosis					1196:1203	the necrosis	1192:1203	the necrosis of hepatocytes	1192:1218	Furthermore, the necrosis of hepatocytes was assessed by means of catalytic activities of liver toxicity markers.
36015417	2	59	dep	membranes	605:613	arg1	viz					616:618	viz	616:618	viz	616:618	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	0	60	theme	Antigenotoxic	124:136	arg1	Features					138:145	In Vivo Hepatoprotective and Antigenotoxic Features	95:145	In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations	95:190	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	1	61	theme	chemical	220:227	arg1	analysis					241:248	the chemical composition analysis	216:248	the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	216:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	10	62	theme	liver	2165:2169	arg1	diseases					2171:2178	toxin-induced liver diseases	2151:2178	toxin-induced liver diseases	2151:2178	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	7	63	theme	comet	1356:1360	arg1	assay					1362:1366	the comet assay	1352:1366	the comet assay	1352:1366	The extracts' antigenotoxic features were quantified using the comet assay.
36015417	6	64	theme	toxicity	1275:1282	arg1	markers					1284:1290	liver toxicity markers	1269:1290	liver toxicity markers	1269:1290	Furthermore, the necrosis of hepatocytes was assessed by means of catalytic activities of liver toxicity markers.
36015417	4	65	from	properties	889:898	arg1	concentrations					907:920	the concentrations	903:920	the concentrations of 200, 100, and 50 mg/kg of body weight	903:961	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	8	66	from	abundant	1686:1693	arg1	SME					1698:1700	SME	1698:1700	SME	1698:1700	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	0	67	theme	Modeling	168:175	arg1	Investigations					177:190	Molecular Modeling Investigations	158:190	In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations	95:190	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	1	68	theme	features	394:401	arg1	analysis					241:248	the chemical composition analysis	216:248	the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features	216:401	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	2	69	theme	radicals	546:553	arg1	generation					490:499	the generation	486:499	the generation of trichloromethyl and trichloromethylperoxy radicals	486:553	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	10	70	theme	N.	2047:2048	arg1	extracts					2058:2065	the examined N. cataria extracts	2034:2065	the examined N. cataria extracts in specified concentrations	2034:2093	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	0	71	theme	Methanol	61:68	arg1	Extracts					70:77	Methanol Extracts	61:77	Methanol Extracts	61:77	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	5	72	theme	hepatoprotective	998:1013	arg1	features					1015:1022	the extracts' hepatoprotective features	984:1022	the extracts' hepatoprotective features	984:1022	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	7	73	theme	antigenotoxic	1307:1319	arg1	features					1321:1328	The extracts' antigenotoxic features	1293:1328	The extracts' antigenotoxic features	1293:1328	The extracts' antigenotoxic features were quantified using the comet assay.
36015417	5	74	theme	enzymes	1082:1088	arg1	concentrations					1098:1111	the concentrations	1094:1111	the concentrations of reactive oxidative species, modulating the liver redox status	1094:1176	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	5	74	theme	enzymes	1082:1088	arg1	activities					1068:1077	the catalytic activities	1054:1077	the catalytic activities of enzymes	1054:1088	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	9	75	dep	which	1801:1805	arg1	upon					1796:1799	upon	1796:1799	upon	1796:1799	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	2	76	theme	liver	445:449	arg1	intoxication					451:462	Wistar rat liver intoxication	434:462	Wistar rat liver intoxication with CCl4	434:472	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	10	77	theme	specified	2070:2078	arg1	concentrations					2080:2093	specified concentrations	2070:2093	specified concentrations	2070:2093	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	8	78	from	SME	1698:1700	arg1	abundant					1686:1693	abundant	1686:1693	abundant	1686:1693	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	0	79	theme	In	95:96	arg1	Features					138:145	In Vivo Hepatoprotective and Antigenotoxic Features	95:145	In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations	95:190	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	9	80	theme	structure-based	1875:1889	arg1	studies					1891:1897	the structure-based studies	1871:1897	the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break	1871:2017	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	0	81	theme	Hepatoprotective	103:118	arg1	Features					138:145	In Vivo Hepatoprotective and Antigenotoxic Features	95:145	In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations	95:190	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	8	82	theme	abundant	1686:1693	arg1	acid					1504:1507	quinic acid	1497:1507	quinic acid (536.11 μg/g)	1497:1521	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	8	82	theme	abundant	1686:1693	arg1	μg/g					1517:1520	536.11 μg/g	1510:1520	536.11 μg/g	1510:1520	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	0	83	dep	In	95:96	arg1	Vivo					98:101	Vivo	98:101	Vivo	98:101	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	4	84	theme	Examined	757:764	arg1	FME					766:768	FME	766:768	FME	766:768	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	10	85	theme	diseases	2171:2178	arg1	prevention					2137:2146	the prevention	2133:2146	the prevention of toxin-induced liver diseases	2133:2178	Conclusively, the examined N. cataria extracts in specified concentrations could be used in clinical therapy for the prevention of toxin-induced liver diseases.
36015417	0	86	theme	Various	24:30	arg1	Organs					53:58	Various Nepeta cataria Plant Organs	24:58	Various Nepeta cataria Plant Organs	24:58	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	0	87	theme	cataria	39:45	arg1	Organs					53:58	Various Nepeta cataria Plant Organs	24:58	Various Nepeta cataria Plant Organs	24:58	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	1	88	theme	methanol	294:301	arg1	extracts					303:310	stem methanol extracts	289:310	stem methanol extracts	289:310	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
36015417	2	89	theme	Wistar	434:439	arg1	intoxication					451:462	Wistar rat liver intoxication	434:462	Wistar rat liver intoxication with CCl4	434:472	Herein, Wistar rat liver intoxication with CCl4 resulted in the generation of trichloromethyl and trichloromethylperoxy radicals, causing lipid peroxidation within the hepatocyte membranes (viz.
36015417	9	90	theme	redox	1925:1929	arg1	marker					1931:1936	a redox marker	1923:1936	a redox marker	1923:1936	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	0	91	theme	Organs	53:58	arg1	Composition					9:19	Chemical Composition	0:19	Chemical Composition of Various Nepeta cataria Plant Organs	0:58	Chemical Composition of Various Nepeta cataria Plant Organs' Methanol Extracts Associated with In Vivo Hepatoprotective and Antigenotoxic Features as well as Molecular Modeling Investigations.
36015417	4	92	theme	weight	956:961	arg1	mg/kg					942:946	200, 100, and 50 mg/kg	925:946	200, 100, and 50 mg/kg of body weight	925:961	Examined FME, LME, and SME administered orally to Wistar rats before the injection of CCl4 exerted the most notable pharmacological properties in the concentrations of 200, 100, and 50 mg/kg of body weight, respectively.
36015417	8	93	theme	Distinct	1369:1376	arg1	features					1403:1410	Distinct pharmacological property features	1369:1410	Distinct pharmacological property features	1369:1410	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	5	94	theme	oxidative	1125:1133	arg1	species					1135:1141	reactive oxidative species	1116:1141	reactive oxidative species	1116:1141	Thus, the extracts' hepatoprotective features were determined by monitoring the catalytic activities of enzymes and the concentrations of reactive oxidative species, modulating the liver redox status.
36015417	6	95	theme	markers	1284:1290	arg1	activities					1255:1264	catalytic activities	1245:1264	catalytic activities of liver toxicity markers	1245:1290	Furthermore, the necrosis of hepatocytes was assessed by means of catalytic activities of liver toxicity markers.
36015417	9	96	theme	rat	1943:1945	arg1	topoisomerase					1947:1959	rat topoisomerase IIα	1943:1963	rat topoisomerase IIα	1943:1963	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	8	97	theme	property	1394:1401	arg1	features					1403:1410	Distinct pharmacological property features	1369:1410	Distinct pharmacological property features	1369:1410	Distinct pharmacological property features may be attributed to quercitrin (8406.31 μg/g), chlorogenic acid (1647.32 μg/g), and quinic acid (536.11 μg/g), found within the FME, rosmarinic acid (1056.14 μg/g), and chlorogenic acid (648.52 μg/g), occurring within the LME, and chlorogenic acid (1408.43 μg/g), the most abundant in SME.
36015417	9	98	theme	secondary	1722:1730	arg1	metabolites					1732:1742	the plant's secondary metabolites	1710:1742	the plant's secondary metabolites	1710:1742	Hence, the plant's secondary metabolites were individually administered similar to extracts, upon which their pharmacology in vivo was elucidated in silico by means of the structure-based studies within rat catalase, as a redox marker, and rat topoisomerase IIα, an enzyme catalyzing the rat DNA double-strand break.
36015417	3	99	theme	aberrant	677:684	arg1	adducts					691:697	aberrant rDNA adducts	677:697	aberrant rDNA adducts	677:697	hepatotoxicity), as well as the subsequent formation of aberrant rDNA adducts and consequent double-strand break (namely genotoxicity).
36015417	1	100	theme	cataria	260:266	arg1	flower					271:276	Nepeta cataria L. flower	253:276	Nepeta cataria L. flower	253:276	This report summarizes the chemical composition analysis of Nepeta cataria L. flower, leaf, and stem methanol extracts (FME, LME, SME, respectively) as well as their hepatoprotective and antigenotoxic features in vivo and in silico.
37297481	3	0	theme	crushed	644:650	arg1	grapes					652:657	the crushed grapes	640:657	the crushed grapes using industrial-scale equipment	640:690	Wines were elaborated with Monastrell, Syrah and Cabernet Sauvignon grapes, applying a sonication treatment to the crushed grapes using industrial-scale equipment.
37297481	5	1	dep	Syrah	767:771	arg1	grapes					796:801	grapes	796:801	grapes	796:801	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	2	2	theme	biochemical	312:322	arg1	differences					324:334	the biochemical differences	308:334	the biochemical differences that exist between the cell walls of different grape varieties	308:397	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	0	3	theme	Technique	57:65	arg1	Dependent					67:75	Dependent	67:75	Dependent	67:75	High-Power Ultrasound in Enology: Is the Outcome of This Technique Dependent on Grape Variety?
37297481	0	3	theme	Technique	57:65	arg1	Outcome					41:47	the Outcome	37:47	the Outcome of This Technique	37:65	High-Power Ultrasound in Enology: Is the Outcome of This Technique Dependent on Grape Variety?
37297481	0	4	from	Ultrasound	11:20	arg1	Enology					25:31	Enology	25:31	Enology	25:31	High-Power Ultrasound in Enology: Is the Outcome of This Technique Dependent on Grape Variety?
37297481	6	5	theme	biochemical	1261:1271	arg1	characteristics					1273:1287	biochemical characteristics	1261:1287	biochemical characteristics associated with a greater rigidity and firmness of the structures	1261:1353	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	5	6	theme	color	849:853	arg1	intensity					855:863	color intensity	849:863	color intensity	849:863	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	1	7	theme	chromatic	215:223	arg1	improvement					249:259	the chromatic, aromatic and mouthfeel improvement	211:259	the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines	211:299	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	0	8	theme	Grape	80:84	arg1	Variety					86:92	Grape Variety	80:92	Grape Variety	80:92	High-Power Ultrasound in Enology: Is the Outcome of This Technique Dependent on Grape Variety?
37297481	5	9	theme	polysaccharides	1102:1116	arg1	families					1090:1097	different families	1080:1097	different families of polysaccharides	1080:1116	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	4	10	theme	clear	714:718	arg1	effect					729:734	a clear varietal effect	712:734	a clear varietal effect	712:734	The results showed a clear varietal effect.
37297481	5	11	theme	phenolic	886:893	arg1	compounds					895:903	phenolic compounds	886:903	phenolic compounds	886:903	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	3	12	theme	industrial-scale	665:680	arg1	equipment					682:690	industrial-scale equipment	665:690	industrial-scale equipment	665:690	Wines were elaborated with Monastrell, Syrah and Cabernet Sauvignon grapes, applying a sonication treatment to the crushed grapes using industrial-scale equipment.
37297481	1	13	theme	aromatic	226:233	arg1	improvement					249:259	the chromatic, aromatic and mouthfeel improvement	211:259	the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines	211:299	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	5	14	theme	highest	1055:1061	arg1	concentration					1063:1075	the highest concentration	1051:1075	the highest concentration in different families of polysaccharides	1051:1116	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	6	15	theme	Monastrell	1233:1242	arg1	grapes					1244:1249	Monastrell grapes	1233:1249	Monastrell grapes	1233:1249	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	3	16	dep	Monastrell	556:565	arg1	grapes					597:602	grapes	597:602	grapes	597:602	Wines were elaborated with Monastrell, Syrah and Cabernet Sauvignon grapes, applying a sonication treatment to the crushed grapes using industrial-scale equipment.
37297481	2	17	theme	cell	359:362	arg1	walls					364:368	the cell walls	355:368	the cell walls of different grape varieties	355:397	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	2	18	theme	US	465:466	arg1	application					450:460	the application	446:460	the application of US in a winery	446:478	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	2	19	from	effect	436:441	arg1	winery					473:478	a winery	471:478	a winery	471:478	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	5	20	theme	different	1080:1088	arg1	families					1090:1097	different families	1080:1097	different families of polysaccharides	1080:1116	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	5	21	from	concentration	869:881	arg1	values					839:844	the values	835:844	the values of color intensity	835:863	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	4	22	theme	varietal	720:727	arg1	effect					729:734	a clear varietal effect	712:734	a clear varietal effect	712:734	The results showed a clear varietal effect.
37297481	5	23	theme	important	813:821	arg1	increase					823:830	an important increase	810:830	an important increase in the values of color intensity	810:863	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	5	24	from	concentration	1063:1075	arg1	families					1090:1097	different families	1080:1097	different families of polysaccharides	1080:1116	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	1	25	dep	ultrasound	139:148	arg1	US					151:152	US	151:152	US	151:152	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	6	26	from	differences	1153:1163	arg1	structure					1188:1196	structure	1188:1196	structure	1188:1196	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	6	26	from	differences	1153:1163	arg1	composition					1172:1182	composition	1172:1182	composition	1172:1182	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	0	27	theme	High-Power	0:9	arg1	Ultrasound					11:20	High-Power Ultrasound	0:20	High-Power Ultrasound in Enology	0:31	High-Power Ultrasound in Enology: Is the Outcome of This Technique Dependent on Grape Variety?
37297481	5	28	theme	compounds	895:903	arg1	concentration					869:881	concentration	869:881	concentration of phenolic compounds	869:903	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	5	28	theme	compounds	895:903	arg1	increase					823:830	an important increase	810:830	an important increase in the values of color intensity	810:863	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	1	29	theme	grape	158:162	arg1	walls					169:173	grape cell walls	158:173	grape cell walls	158:173	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	6	30	dep	greater	1307:1313	arg1	firmness					1328:1335	firmness	1328:1335	firmness	1328:1335	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	6	30	dep	greater	1307:1313	arg1	rigidity					1315:1322	rigidity	1315:1322	rigidity	1315:1322	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	1	31	theme	mouthfeel	239:247	arg1	improvement					249:259	the chromatic, aromatic and mouthfeel improvement	211:259	the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines	211:299	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	2	32	theme	grape	383:387	arg1	varieties					389:397	different grape varieties	373:397	different grape varieties	373:397	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	2	33	theme	varieties	389:397	arg1	walls					364:368	the cell walls	355:368	the cell walls of different grape varieties	355:397	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	2	34	theme	different	373:381	arg1	varieties					389:397	different grape varieties	373:397	different grape varieties	373:397	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	5	35	theme	Monastrell	1024:1033	arg1	wines					1035:1039	Monastrell wines	1024:1039	Monastrell wines	1024:1039	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	6	36	theme	walls	1212:1216	arg1	structure					1188:1196	structure	1188:1196	structure	1188:1196	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	6	36	theme	walls	1212:1216	arg1	composition					1172:1182	composition	1172:1182	composition	1172:1182	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	6	37	theme	cell	1207:1210	arg1	walls					1212:1216	their cell walls	1201:1216	their cell walls	1201:1216	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	6	38	dep	composition	1172:1182	arg1	the					1168:1170	the	1168:1170	the	1168:1170	These findings correlate with the differences in the composition and structure of their cell walls since those of Monastrell grapes presented biochemical characteristics associated with a greater rigidity and firmness of the structures.
37297481	2	39	from	application	450:460	arg1	winery					473:478	a winery	471:478	a winery	471:478	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	5	40	theme	intensity	855:863	arg1	values					839:844	the values	835:844	the values of color intensity	835:863	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	1	41	theme	red	291:293	arg1	wines					295:299	red wines	291:299	red wines	291:299	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	5	42	theme	Monastrell	989:998	arg1	grapes					1008:1013	Monastrell crushed grapes	989:1013	Monastrell crushed grapes	989:1013	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	5	43	theme	crushed	1000:1006	arg1	grapes					1008:1013	Monastrell crushed grapes	989:1013	Monastrell crushed grapes	989:1013	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	1	44	theme	disruptive	99:108	arg1	effect					110:115	The disruptive effect	95:115	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls	95:173	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	1	44	theme	disruptive	99:108	arg1	reason					197:202	the reason	193:202	the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines	193:299	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	2	45	theme	grape	506:510	arg1	variety					512:518	the grape variety	502:518	the grape variety treated	502:526	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	3	46	theme	sonication	616:625	arg1	treatment					627:635	a sonication treatment	614:635	a sonication treatment	614:635	Wines were elaborated with Monastrell, Syrah and Cabernet Sauvignon grapes, applying a sonication treatment to the crushed grapes using industrial-scale equipment.
37297481	2	47	from	winery	473:478	arg1	effect					436:441	the effect	432:441	the effect of the application of US in a winery	432:478	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	5	48	from	increase	823:830	arg1	values					839:844	the values	835:844	the values of color intensity	835:863	The wines made with sonicated Syrah and Cabernet Sauvignon grapes showed an important increase in the values of color intensity and concentration of phenolic compounds, and these increases were higher than those observed when sonication was applied to Monastrell crushed grapes, whereas Monastrell wines presented the highest concentration in different families of polysaccharides.
37297481	1	49	theme	cell	164:167	arg1	walls					169:173	grape cell walls	158:173	grape cell walls	158:173	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	2	50	theme	application	450:460	arg1	effect					436:441	the effect	432:441	the effect of the application of US in a winery	432:478	Given the biochemical differences that exist between the cell walls of different grape varieties, this paper investigates whether the effect of the application of US in a winery may vary according to the grape variety treated.
37297481	1	51	dep	improvement	249:259	arg1	causes					281:286	causes	281:286	causes in red wines	281:299	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
37297481	1	52	theme	high-power	128:137	arg1	ultrasound					139:148	high-power ultrasound	128:148	high-power ultrasound (US)	128:153	The disruptive effect exerted by high-power ultrasound (US) on grape cell walls is established as the reason behind the chromatic, aromatic and mouthfeel improvement that this treatment causes in red wines.
35579149	1	0	theme	contemporary	276:287	arg1	worldwide					298:306	contemporary medicine worldwide	276:306	contemporary medicine worldwide	276:306	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	11	1	theme	PGPs-NE	1391:1397	arg1	shape					1382:1386	The spherical shape	1368:1386	The spherical shape of PGPs-NE	1368:1397	The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm.
35579149	1	2	theme	BACKGROUND	137:146	arg1	cancer					159:164	BACKGROUND Colorectal cancer	137:164	BACKGROUND Colorectal cancer	137:164	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	9	3	from	202	1201:1203	arg1	transmittance					1226:1238	a transmittance	1224:1238	a transmittance of 80%	1224:1245	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	9	3	from	202	1201:1203	arg1	bands					1192:1196	three optical absorption bands	1167:1196	three optical absorption bands at 202, 204 and 207nm	1167:1218	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	13	4	theme	free	1750:1753	arg1	PGPs					1755:1758	free PGPs	1750:1758	free PGPs	1750:1758	The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
35579149	1	5	theme	gastrointestinal	193:208	arg1	cancers					210:216	the most serious gastrointestinal cancers	176:216	the most serious gastrointestinal cancers in Africa	176:226	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	0	6	theme	anti-inflammatory	80:96	arg1	properties					125:134	anti-inflammatory, antioxidant and antitumor properties	80:134	anti-inflammatory, antioxidant and antitumor properties	80:134	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	13	7	theme	agent	1716:1720	arg1	potency					1722:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
35579149	9	8	theme	%	1245:1245	arg1	transmittance					1226:1238	a transmittance	1224:1238	a transmittance of 80%	1224:1245	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	9	8	theme	%	1245:1245	arg1	bands					1192:1196	three optical absorption bands	1167:1196	three optical absorption bands at 202, 204 and 207nm	1167:1218	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	14	9	theme	novel	1851:1855	arg1	PGPs-NE					1857:1863	novel PGPs-NE	1851:1863	novel PGPs-NE	1851:1863	CONCLUSION These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
35579149	6	10	dep	RESULTS	860:866	arg1	yield					877:881	The PGPs yield	868:881	The PGPs yield	868:881	RESULTS The PGPs yield was 10%.
35579149	6	10	dep	RESULTS	860:866	arg1	%					889:889	10%	887:889	10%	887:889	RESULTS The PGPs yield was 10%.
35579149	4	11	theme	physicochemical	678:692	arg1	characterization					694:709	The physicochemical characterization	674:709	The physicochemical characterization of PGPs-NE	674:720	The physicochemical characterization of PGPs-NE was then analyzed.
35579149	13	12	theme	anti-inflammatory	1683:1699	arg1	potency					1722:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
35579149	3	13	theme	self-emulsification	625:643	arg1	technique					645:653	the self-emulsification technique	621:653	the self-emulsification technique with an oil phase	621:671	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	1	14	from	challenge	263:271	arg1	worldwide					298:306	contemporary medicine worldwide	276:306	contemporary medicine worldwide	276:306	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	1	15	theme	Colorectal	148:157	arg1	cancer					159:164	BACKGROUND Colorectal cancer	137:164	BACKGROUND Colorectal cancer	137:164	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	11	16	theme	average	1466:1472	arg1	size					1474:1477	an average size	1463:1477	an average size of less than 50 nm	1463:1496	The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm.
35579149	3	17	theme	oil	663:665	arg1	phase					667:671	an oil phase	660:671	an oil phase	660:671	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	1	18	from	cancers	210:216	arg1	Africa					221:226	Africa	221:226	Africa	221:226	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	5	19	dep	antioxidant	754:764	arg1	anti-inflammatory					767:783	anti-inflammatory	767:783	anti-inflammatory	767:783	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	9	20	from	207nm	1214:1218	arg1	transmittance					1226:1238	a transmittance	1224:1238	a transmittance of 80%	1224:1245	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	9	20	from	207nm	1214:1218	arg1	bands					1192:1196	three optical absorption bands	1167:1196	three optical absorption bands at 202, 204 and 207nm	1167:1218	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	3	21	theme	PGPs	574:577	arg1	PGPs-NE					593:599	PGPs-NE	593:599	PGPs-NE	593:599	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	3	21	theme	PGPs	574:577	arg1	nanoemulsion					579:590	a PGPs nanoemulsion	572:590	a PGPs nanoemulsion (PGPs-NE)	572:600	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	5	22	dep	in	745:746	arg1	vitro					748:752	vitro	748:752	vitro	748:752	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	9	23	from	204	1206:1208	arg1	transmittance					1226:1238	a transmittance	1224:1238	a transmittance of 80%	1224:1245	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	9	23	from	204	1206:1208	arg1	bands					1192:1196	three optical absorption bands	1167:1196	three optical absorption bands at 202, 204 and 207nm	1167:1218	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	5	24	theme	PGPs	822:825	arg1	potency					811:817	antitumor potency	801:817	antitumor potency of PGPs and PGPs-NE	801:837	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	5	24	theme	PGPs	822:825	arg1	activities					785:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	10	25	with	9.5nm	1291:1295	arg1	potential					1346:1354	a negative zeta potential	1330:1354	a negative zeta potential (-30.6 mV)	1330:1365	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	10	25	with	9.5nm	1291:1295	arg1	mV					1363:1364	-30.6 mV	1357:1364	-30.6 mV	1357:1364	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	10	25	with	9.5nm	1291:1295	arg1	PDI					1305:1307	a PDI	1303:1307	a PDI of less than 0.2	1303:1324	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	14	26	theme	promising	1778:1786	arg1	findings					1796:1803	These promising current findings	1772:1803	These promising current findings	1772:1803	CONCLUSION These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
35579149	13	27	theme	strong	1663:1668	arg1	potency					1722:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
35579149	2	28	dep	using	390:394	arg1	evaluated					466:474	evaluated	466:474	evaluated its biological activities	466:500	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	10	29	theme	hydrodynamic	1260:1271	arg1	size					1282:1285	Its average hydrodynamic particle size	1248:1285	Its average hydrodynamic particle size	1248:1285	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	9	30	theme	formulated	1144:1153	arg1	PGPs-NE					1155:1161	the formulated PGPs-NE	1140:1161	the formulated PGPs-NE	1140:1161	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	13	31	theme	current	1616:1622	arg1	study					1624:1628	The current study	1612:1628	The current study	1612:1628	The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
35579149	2	32	theme	biological	480:489	arg1	activities					491:500	its biological activities	476:500	its biological activities	476:500	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	3	33	with	technique	645:653	arg1	phase					667:671	an oil phase	660:671	an oil phase	660:671	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	10	34	theme	less	1312:1315	arg1	potential					1346:1354	a negative zeta potential	1330:1354	a negative zeta potential (-30.6 mV)	1330:1365	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	10	34	theme	less	1312:1315	arg1	mV					1363:1364	-30.6 mV	1357:1364	-30.6 mV	1357:1364	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	10	34	theme	less	1312:1315	arg1	PDI					1305:1307	a PDI	1303:1307	a PDI of less than 0.2	1303:1324	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	2	35	dep	OBJECTIVE	309:317	arg1	aimed					337:341	aimed	337:341	aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities	337:500	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	11	36	theme	electron	1431:1438	arg1	study					1451:1455	a transmission electron microscope study	1416:1455	a transmission electron microscope study	1416:1455	The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm.
35579149	2	37	theme	cancer	446:451	arg1	remedy					453:458	alternative cancer remedy	434:458	alternative cancer remedy	434:458	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	0	38	theme	pomegranate	23:33	arg1	formulation					63:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	5	39	theme	PGPs-NE	831:837	arg1	potency					811:817	antitumor potency	801:817	antitumor potency of PGPs and PGPs-NE	801:837	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	5	39	theme	PGPs-NE	831:837	arg1	activities					785:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	2	40	theme	delivery	374:381	arg1	system					383:388	a nanoemulsion drug delivery system	354:388	a nanoemulsion drug delivery system	354:388	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	0	41	theme	nanoemulsion	50:61	arg1	formulation					63:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	0	42	with	Development	0:10	arg1	properties					125:134	anti-inflammatory, antioxidant and antitumor properties	80:134	anti-inflammatory, antioxidant and antitumor properties	80:134	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	2	43	theme	nanoemulsion	356:367	arg1	system					383:388	a nanoemulsion drug delivery system	354:388	a nanoemulsion drug delivery system	354:388	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	7	44	theme	PGPs	931:934	arg1	sugar					902:906	The total sugar and protein content	892:926	sugar	902:906	The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83µg/ml, respectively.
35579149	7	44	theme	PGPs	931:934	arg1	content					920:926	The total sugar and protein content	892:926	content	920:926	The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83µg/ml, respectively.
35579149	7	44	theme	PGPs	931:934	arg1	mg/dl					946:950	44.66 mg/dl	940:950	44.66 mg/dl	940:950	The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83µg/ml, respectively.
35579149	1	45	theme	pronounced	252:261	arg1	challenge					263:271	a pronounced challenge	250:271	a pronounced challenge in contemporary medicine worldwide	250:306	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	1	45	theme	pronounced	252:261	arg1	prevention					236:245	its prevention	232:245	its prevention	232:245	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	10	46	theme	zeta	1341:1344	arg1	potential					1346:1354	a negative zeta potential	1330:1354	a negative zeta potential (-30.6 mV)	1330:1365	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	10	46	theme	zeta	1341:1344	arg1	mV					1363:1364	-30.6 mV	1357:1364	-30.6 mV	1357:1364	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	14	47	theme	alternative	1871:1881	arg1	treatment					1883:1891	an alternative treatment	1868:1891	an alternative treatment for CRC	1868:1899	CONCLUSION These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
35579149	2	48	theme	present	323:329	arg1	study					331:335	The present study	319:335	The present study	319:335	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	1	49	theme	medicine	289:296	arg1	worldwide					298:306	contemporary medicine worldwide	276:306	contemporary medicine worldwide	276:306	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	11	50	theme	spherical	1372:1380	arg1	shape					1382:1386	The spherical shape	1368:1386	The spherical shape of PGPs-NE	1368:1397	The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm.
35579149	11	51	theme	nm	1495:1496	arg1	size					1474:1477	an average size	1463:1477	an average size of less than 50 nm	1463:1496	The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm.
35579149	7	52	theme	total	896:900	arg1	sugar					902:906	The total sugar and protein content	892:926	sugar	902:906	The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83µg/ml, respectively.
35579149	7	52	theme	total	896:900	arg1	mg/dl					946:950	44.66 mg/dl	940:950	44.66 mg/dl	940:950	The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83µg/ml, respectively.
35579149	1	53	theme	serious	185:191	arg1	cancers					210:216	the most serious gastrointestinal cancers	176:216	the most serious gastrointestinal cancers in Africa	176:226	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	13	54	theme	antitumor	1706:1714	arg1	potency					1722:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
35579149	1	55	theme	cancers	210:216	arg1	cancers					210:216	the most serious gastrointestinal cancers	176:216	the most serious gastrointestinal cancers in Africa	176:226	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	1	55	theme	cancers	210:216	arg1	one					169:171	one	169:171	one	169:171	BACKGROUND Colorectal cancer is one of the most serious gastrointestinal cancers in Africa and its prevention is a pronounced challenge in contemporary medicine worldwide.
35579149	4	56	theme	PGPs-NE	714:720	arg1	characterization					694:709	The physicochemical characterization	674:709	The physicochemical characterization of PGPs-NE	674:720	The physicochemical characterization of PGPs-NE was then analyzed.
35579149	5	57	theme	in	745:746	arg1	activities					785:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	14	58	theme	PGPs-NE	1857:1863	arg1	efficacy					1839:1846	the possible efficacy	1826:1846	the possible efficacy of novel PGPs-NE as an alternative treatment for CRC	1826:1899	CONCLUSION These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
35579149	12	59	theme	high	1575:1578	arg1	efficiency					1591:1600	a high entrapment efficiency	1573:1600	a high entrapment efficiency (92.82%)	1573:1609	Additionally, the method used to prepare the PGPs-NE formulation provided a high entrapment efficiency (92.82%).
35579149	12	59	theme	high	1575:1578	arg1	%					1608:1608	92.82%	1603:1608	92.82%	1603:1608	Additionally, the method used to prepare the PGPs-NE formulation provided a high entrapment efficiency (92.82%).
35579149	2	60	theme	drug	369:372	arg1	system					383:388	a nanoemulsion drug delivery system	354:388	a nanoemulsion drug delivery system	354:388	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	5	61	theme	antioxidant	754:764	arg1	activities					785:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities	741:794	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	14	62	theme	possible	1830:1837	arg1	efficacy					1839:1846	the possible efficacy	1826:1846	the possible efficacy of novel PGPs-NE as an alternative treatment for CRC	1826:1899	CONCLUSION These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
35579149	12	63	theme	PGPs-NE	1544:1550	arg1	formulation					1552:1562	the PGPs-NE formulation	1540:1562	the PGPs-NE formulation	1540:1562	Additionally, the method used to prepare the PGPs-NE formulation provided a high entrapment efficiency (92.82%).
35579149	10	64	theme	average	1252:1258	arg1	size					1282:1285	Its average hydrodynamic particle size	1248:1285	Its average hydrodynamic particle size	1248:1285	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	14	65	dep	CONCLUSION	1761:1770	arg1	provide					1805:1811	provide	1805:1811	provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC	1805:1899	CONCLUSION These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
35579149	9	66	theme	absorption	1181:1190	arg1	bands					1192:1196	three optical absorption bands	1167:1196	three optical absorption bands at 202, 204 and 207nm	1167:1218	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	3	67	theme	PGPs	515:518	arg1	METHODS					503:509	METHODS	503:509	METHODS The PGPs yield and chemical composition	503:549	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	3	67	theme	PGPs	515:518	arg1	yield					520:524	The PGPs yield	511:524	The PGPs yield	511:524	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	5	68	theme	antitumor	801:809	arg1	potency					811:817	antitumor potency	801:817	antitumor potency of PGPs and PGPs-NE	801:837	The in vitro antioxidant, anti-inflammatory activities, and antitumor potency of PGPs and PGPs-NE were also evaluated.
35579149	0	69	theme	antioxidant	99:109	arg1	properties					125:134	anti-inflammatory, antioxidant and antitumor properties	80:134	anti-inflammatory, antioxidant and antitumor properties	80:134	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	14	70	theme	current	1788:1794	arg1	findings					1796:1803	These promising current findings	1772:1803	These promising current findings	1772:1803	CONCLUSION These promising current findings provide evidence for the possible efficacy of novel PGPs-NE as an alternative treatment for CRC.
35579149	10	71	theme	particle	1273:1280	arg1	size					1282:1285	Its average hydrodynamic particle size	1248:1285	Its average hydrodynamic particle size	1248:1285	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	0	72	theme	antitumor	115:123	arg1	properties					125:134	anti-inflammatory, antioxidant and antitumor properties	80:134	anti-inflammatory, antioxidant and antitumor properties	80:134	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	13	73	theme	antioxidant	1670:1680	arg1	potency					1722:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	strong antioxidant, anti-inflammatory, and antitumor agent potency	1663:1728	The current study disclosed that PGPs-NE exhibited strong antioxidant, anti-inflammatory, and antitumor agent potency compared to that of free PGPs.
35579149	9	74	theme	physiochemical	1107:1120	arg1	characterization					1122:1137	physiochemical characterization	1107:1137	physiochemical characterization	1107:1137	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	11	75	theme	microscope	1440:1449	arg1	study					1451:1455	a transmission electron microscope study	1416:1455	a transmission electron microscope study	1416:1455	The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm.
35579149	0	76	theme	novel	17:21	arg1	formulation					63:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	12	77	theme	entrapment	1580:1589	arg1	efficiency					1591:1600	a high entrapment efficiency	1573:1600	a high entrapment efficiency (92.82%)	1573:1609	Additionally, the method used to prepare the PGPs-NE formulation provided a high entrapment efficiency (92.82%).
35579149	12	77	theme	entrapment	1580:1589	arg1	%					1608:1608	92.82%	1603:1608	92.82%	1603:1608	Additionally, the method used to prepare the PGPs-NE formulation provided a high entrapment efficiency (92.82%).
35579149	11	78	theme	transmission	1418:1429	arg1	study					1451:1455	a transmission electron microscope study	1416:1455	a transmission electron microscope study	1416:1455	The spherical shape of PGPs-NE was confirmed by a transmission electron microscope study, with an average size of less than 50 nm.
35579149	0	79	theme	polysaccharide	35:48	arg1	formulation					63:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	a novel pomegranate polysaccharide nanoemulsion formulation	15:73	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	9	80	contain	had	1163:1165	arg2	transmittance					1226:1238	a transmittance	1224:1238	a transmittance of 80%	1224:1245	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	9	80	contain	had	1163:1165	arg2	bands					1192:1196	three optical absorption bands	1167:1196	three optical absorption bands at 202, 204 and 207nm	1167:1218	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	9	80	contain	had	1163:1165	arg1	PGPs-NE					1155:1161	the formulated PGPs-NE	1140:1161	the formulated PGPs-NE	1140:1161	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	10	81	theme	negative	1332:1339	arg1	potential					1346:1354	a negative zeta potential	1330:1354	a negative zeta potential (-30.6 mV)	1330:1365	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	10	81	theme	negative	1332:1339	arg1	mV					1363:1364	-30.6 mV	1357:1364	-30.6 mV	1357:1364	Its average hydrodynamic particle size was 9.5nm, with a PDI of less than 0.2 and a negative zeta potential (-30.6 mV).
35579149	2	82	theme	alternative	434:444	arg1	remedy					453:458	alternative cancer remedy	434:458	alternative cancer remedy	434:458	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	0	83	theme	formulation	63:73	arg1	Development					0:10	Development	0:10	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.	0:135	Development of a novel pomegranate polysaccharide nanoemulsion formulation with anti-inflammatory, antioxidant and antitumor properties.
35579149	9	84	theme	optical	1173:1179	arg1	bands					1192:1196	three optical absorption bands	1167:1196	three optical absorption bands at 202, 204 and 207nm	1167:1218	Concerning physiochemical characterization, the formulated PGPs-NE had three optical absorption bands at 202, 204 and 207nm and a transmittance of 80%.
35579149	3	85	theme	chemical	530:537	arg1	METHODS					503:509	METHODS	503:509	METHODS The PGPs yield and chemical composition	503:549	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	3	85	theme	chemical	530:537	arg1	composition					539:549	chemical composition	530:549	chemical composition	530:549	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	6	86	theme	PGPs	872:875	arg1	yield					877:881	The PGPs yield	868:881	The PGPs yield	868:881	RESULTS The PGPs yield was 10%.
35579149	6	86	theme	PGPs	872:875	arg1	%					889:889	10%	887:889	10%	887:889	RESULTS The PGPs yield was 10%.
35579149	3	87	dep	METHODS	503:509	arg1	METHODS					503:509	METHODS	503:509	METHODS The PGPs yield and chemical composition	503:549	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	3	87	dep	METHODS	503:509	arg1	yield					520:524	The PGPs yield	511:524	The PGPs yield	511:524	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	3	87	dep	METHODS	503:509	arg1	composition					539:549	chemical composition	530:549	chemical composition	530:549	METHODS The PGPs yield and chemical composition were evaluated, then a PGPs nanoemulsion (PGPs-NE) was prepared using the self-emulsification technique with an oil phase.
35579149	2	88	theme	pomegranate	396:406	arg1	PGPs					425:428	PGPs	425:428	PGPs	425:428	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	2	88	theme	pomegranate	396:406	arg1	polysaccharides					408:422	pomegranate polysaccharides	396:422	pomegranate polysaccharides (PGPs)	396:429	OBJECTIVE The present study aimed to develop a nanoemulsion drug delivery system using pomegranate polysaccharides (PGPs) as alternative cancer remedy, then evaluated its biological activities.
35579149	7	89	theme	protein	912:918	arg1	content					920:926	The total sugar and protein content	892:926	content	920:926	The total sugar and protein content of PGPs was 44.66 mg/dl and 19.83µg/ml, respectively.
35247785	6	0	theme	cysteine	987:994	arg1	%					1058:1058	7.5%	1055:1058	7.5%	1055:1058	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	6	0	theme	cysteine	987:994	arg1	percentage					973:982	The percentage	969:982	The percentage of cysteine at the second position relative to modified asparagine	969:1049	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	2	1	from	proteins	423:430	arg1	total					381:385	a total	379:385	a total of 1188 N-glycosylated sites on 626 proteins	379:430	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	7	2	contain	had	1206:1208	arg1	which					1200:1204	which	1200:1204	which	1200:1204	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	7	2	contain	had	1206:1208	arg2	activity					1257:1264	significantly upregulated metalloendopeptidase activity	1210:1264	significantly upregulated metalloendopeptidase activity	1210:1264	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	7	2	contain	had	1206:1208	arg2	binding					1280:1286	metal ion binding	1270:1286	metal ion binding	1270:1286	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	2	3	theme	N-linked	352:359	arg1	glycoproteome					361:373	the first bovine sperm N-linked glycoproteome	329:373	the first bovine sperm N-linked glycoproteome	329:373	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	9	4	theme	cysteine	1523:1530	arg1	residues					1532:1539	ADAM-containing conserved free cysteine residues	1492:1539	ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs	1492:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	10	5	theme	sperm	1691:1695	arg1	glycoproteins					1697:1709	almost all bovine sperm glycoproteins	1673:1709	almost all bovine sperm glycoproteins	1673:1709	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	3	6	theme	lysosome	573:580	arg1	"					581:581	"lysosome"	572:581	"lysosome"	572:581	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	1	7	theme	recognition	279:289	arg1	process					241:247	the whole process	231:247	the whole process of spermatogenesis, sperm-egg recognition, and fertilization	231:308	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	4	8	theme	canonical	753:761	arg1	motifs					779:784	the canonical N-glycosylation motifs	749:784	the canonical N-glycosylation motifs	749:784	Notably, cysteines were highly enriched in the canonical N-glycosylation motifs N-!
35247785	6	9	link	N-linked	1110:1117	arg1	glycoproteome					1119:1131	the previously reported N-linked glycoproteome	1086:1131	the previously reported N-linked glycoproteome	1086:1131	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	7	10	theme	enriched	1158:1165	arg1	N-glycoproteins					1167:1181	120 cysteine enriched N-glycoproteins	1145:1181	120 cysteine enriched N-glycoproteins	1145:1181	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	1	11	theme	proteins	190:197	arg1	modifications					173:185	the most important post-translational modifications	135:185	the most important post-translational modifications of proteins	135:197	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	11	12	theme	new	1922:1924	arg1	means					1926:1930	a new means	1920:1930	a new means to explore potential biomarkers for improving sperm quality and fertility	1920:2004	The mapping of N-glycosylation on bovine sperm may provide a new means to explore potential biomarkers for improving sperm quality and fertility.
35247785	1	13	theme	irreplaceable	209:221	arg1	role					223:226	an irreplaceable role	206:226	an irreplaceable role	206:226	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	5	14	theme	extraordinary	907:919	arg1	roles					921:925	extraordinary roles	907:925	extraordinary roles	907:925	P-[S/T/C] and the conservative motifs N-C-[S/T] were also significantly enriched, indicating these modifications play extraordinary roles in bovine spermatogenesis and maturation.
35247785	8	15	contain	had	1402:1404	arg2	ADAM					1443:1446	metalloproteinase (ADAM)	1424:1447	metalloproteinase (ADAM)	1424:1447	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	8	15	contain	had	1402:1404	arg2	disintegrin					1408:1418	a disintegrin	1406:1418	a disintegrin	1406:1418	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	8	15	contain	had	1402:1404	arg1	15					1352:1353	15	1352:1353	15	1352:1353	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	8	15	contain	had	1402:1404	arg1	glycoproteins					1388:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	10	16	theme	eventual	1837:1844	arg1	fertilization					1846:1858	eventual fertilization	1837:1858	eventual fertilization	1837:1858	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	10	17	gly	glycoproteins	1697:1709	arg1	glycoproteins					1697:1709	almost all bovine sperm glycoproteins	1673:1709	almost all bovine sperm glycoproteins	1673:1709	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	8	18	theme	glycoproteins	1388:1400	arg1	glycoproteins					1388:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	8	18	theme	glycoproteins	1388:1400	arg1	15					1352:1353	15	1352:1353	15	1352:1353	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	11	19	theme	potential	1943:1951	arg1	biomarkers					1953:1962	potential biomarkers	1943:1962	potential biomarkers for improving sperm quality and fertility	1943:2004	The mapping of N-glycosylation on bovine sperm may provide a new means to explore potential biomarkers for improving sperm quality and fertility.
35247785	3	20	theme	component	608:616	arg1	annotation					618:627	cellular component annotation	599:627	cellular component annotation	599:627	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	10	21	theme	sperm-egg	1810:1818	arg1	recognition					1820:1830	sperm-egg recognition	1810:1830	sperm-egg recognition	1810:1830	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	7	22	theme	sperm	1319:1323	arg1	glycoproteome					1325:1337	the whole bovine sperm glycoproteome	1302:1337	the whole bovine sperm glycoproteome	1302:1337	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	1	23	theme	important	144:152	arg1	modifications					173:185	the most important post-translational modifications	135:185	the most important post-translational modifications of proteins	135:197	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	6	24	theme	higher	1066:1071	arg1	%					1058:1058	7.5%	1055:1058	7.5%	1055:1058	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	6	24	theme	higher	1066:1071	arg1	percentage					973:982	The percentage	969:982	The percentage of cysteine at the second position relative to modified asparagine	969:1049	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	5	25	theme	motifs	820:825	arg1	N-C-[S/T					827:834	the conservative motifs N-C-[S/T	803:834	the conservative motifs N-C-[S/T	803:834	P-[S/T/C] and the conservative motifs N-C-[S/T] were also significantly enriched, indicating these modifications play extraordinary roles in bovine spermatogenesis and maturation.
35247785	1	26	theme	modifications	173:185	arg1	Glycosylation					113:125	Glycosylation	113:125	Glycosylation	113:125	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	1	26	theme	modifications	173:185	arg1	one					128:130	one	128:130	one	128:130	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	1	26	theme	modifications	173:185	arg1	modifications					173:185	the most important post-translational modifications	135:185	the most important post-translational modifications of proteins	135:197	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	0	27	theme	spermatozoa	84:94	arg1	N-Glycoproteome					96:110	bovine spermatozoa N-Glycoproteome	77:110	bovine spermatozoa N-Glycoproteome	77:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	4	28	dep	enriched	737:744	arg1	!					788:788	N-!	786:788	N-!	786:788	Notably, cysteines were highly enriched in the canonical N-glycosylation motifs N-!
35247785	2	29	gly	1188 N-glycosylated	390:408	arg1	sites					410:414	1188 N-glycosylated sites	390:414	1188 N-glycosylated sites on 626 proteins	390:430	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	2	30	theme	sites	410:414	arg1	total					381:385	a total	379:385	a total of 1188 N-glycosylated sites on 626 proteins	379:430	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	11	31	theme	bovine	1895:1900	arg1	sperm					1902:1906	bovine sperm	1895:1906	bovine sperm	1895:1906	The mapping of N-glycosylation on bovine sperm may provide a new means to explore potential biomarkers for improving sperm quality and fertility.
35247785	11	32	theme	sperm	1978:1982	arg1	quality					1984:1990	sperm quality	1978:1990	sperm quality	1978:1990	The mapping of N-glycosylation on bovine sperm may provide a new means to explore potential biomarkers for improving sperm quality and fertility.
35247785	7	33	theme	metal	1270:1274	arg1	binding					1280:1286	metal ion binding	1270:1286	metal ion binding	1270:1286	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	3	34	theme	bovine	487:492	arg1	proteins					515:522	bovine sperm N-glycosylated proteins	487:522	bovine sperm N-glycosylated proteins	487:522	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	1	35	theme	fertilization	296:308	arg1	process					241:247	the whole process	231:247	the whole process of spermatogenesis, sperm-egg recognition, and fertilization	231:308	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	6	36	theme	reported	1101:1108	arg1	glycoproteome					1119:1131	the previously reported N-linked glycoproteome	1086:1131	the previously reported N-linked glycoproteome	1086:1131	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	9	37	contain	have	1610:1613	arg2	roles					1629:1633	extraordinary roles	1615:1633	extraordinary roles	1615:1633	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	9	37	contain	have	1610:1613	arg1	residues					1532:1539	ADAM-containing conserved free cysteine residues	1492:1539	ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs	1492:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	7	38	theme	upregulated	1224:1234	arg1	activity					1257:1264	significantly upregulated metalloendopeptidase activity	1210:1264	significantly upregulated metalloendopeptidase activity	1210:1264	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	0	39	theme	glycosylation	54:66	arg1	motif					68:72	the canonical N-linked glycosylation motif	31:72	the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome	31:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	2	40	theme	sperm	346:350	arg1	glycoproteome					361:373	the first bovine sperm N-linked glycoproteome	329:373	the first bovine sperm N-linked glycoproteome	329:373	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	0	41	theme	canonical	35:43	arg1	motif					68:72	the canonical N-linked glycosylation motif	31:72	the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome	31:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	8	42	dep	disintegrin	1408:1418	arg1	domain					1457:1462	protein domain	1449:1462	protein domain	1449:1462	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	2	43	from	sites	410:414	arg1	proteins					423:430	626 proteins	419:430	626 proteins	419:430	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	3	44	theme	N-glycosylated	500:513	arg1	proteins					515:522	bovine sperm N-glycosylated proteins	487:522	bovine sperm N-glycosylated proteins	487:522	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	9	45	theme	bovine	1638:1643	arg1	spermatozoa					1645:1655	bovine spermatozoa	1638:1655	bovine spermatozoa	1638:1655	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	3	46	theme	extracellular	546:558	arg1	"					566:566	"extracellular region"	545:566	"extracellular region"	545:566	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	9	47	theme	glycoprotein	1553:1564	arg1	motifs					1566:1571	N-linked glycoprotein motifs	1544:1571	N-linked glycoprotein motifs	1544:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	9	48	theme	ADAM-containing	1492:1506	arg1	residues					1532:1539	ADAM-containing conserved free cysteine residues	1492:1539	ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs	1492:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	3	49	with	glycoproteins	647:659	arg1	functions					695:703	proteolytic and reproductive functions	666:703	proteolytic and reproductive functions	666:703	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	4	50	from	enriched	737:744	arg1	motifs					779:784	the canonical N-glycosylation motifs	749:784	the canonical N-glycosylation motifs	749:784	Notably, cysteines were highly enriched in the canonical N-glycosylation motifs N-!
35247785	1	51	theme	spermatogenesis	252:266	arg1	process					241:247	the whole process	231:247	the whole process of spermatogenesis, sperm-egg recognition, and fertilization	231:308	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	7	52	theme	N-glycoproteins	1167:1181	arg1	total					1136:1140	A total	1134:1140	A total of 120 cysteine enriched N-glycoproteins	1134:1181	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	1	53	theme	sperm-egg	269:277	arg1	recognition					279:289	sperm-egg recognition	269:289	sperm-egg recognition	269:289	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	10	54	theme	bovine	1684:1689	arg1	glycoproteins					1697:1709	almost all bovine sperm glycoproteins	1673:1709	almost all bovine sperm glycoproteins	1673:1709	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	2	55	link	N-linked	352:359	arg1	glycoproteome					361:373	the first bovine sperm N-linked glycoproteome	329:373	the first bovine sperm N-linked glycoproteome	329:373	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	7	56	theme	cysteine	1149:1156	arg1	N-glycoproteins					1167:1181	120 cysteine enriched N-glycoproteins	1145:1181	120 cysteine enriched N-glycoproteins	1145:1181	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	3	57	theme	"	566:566	arg1	groups					583:588	"extracellular region" and "lysosome" groups	545:588	"extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions	545:703	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	9	58	theme	N-linked	1544:1551	arg1	motifs					1566:1571	N-linked glycoprotein motifs	1544:1571	N-linked glycoprotein motifs	1544:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	0	59	link	N-linked	45:52	arg1	motif					68:72	the canonical N-linked glycosylation motif	31:72	the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome	31:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	3	60	gly	glycoproteins	647:659	arg1	glycoproteins					647:659	glycoproteins	647:659	glycoproteins with proteolytic and reproductive functions	647:703	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	7	61	theme	whole	1306:1310	arg1	glycoproteome					1325:1337	the whole bovine sperm glycoproteome	1302:1337	the whole bovine sperm glycoproteome	1302:1337	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	5	62	theme	bovine	930:935	arg1	spermatogenesis					937:951	bovine spermatogenesis	930:951	bovine spermatogenesis	930:951	P-[S/T/C] and the conservative motifs N-C-[S/T] were also significantly enriched, indicating these modifications play extraordinary roles in bovine spermatogenesis and maturation.
35247785	10	63	theme	enzyme	1716:1721	arg1	activity					1723:1730	enzyme activity	1716:1730	enzyme activity	1716:1730	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	3	64	theme	"	581:581	arg1	groups					583:588	"extracellular region" and "lysosome" groups	545:588	"extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions	545:703	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	6	65	theme	modified	1031:1038	arg1	asparagine					1040:1049	modified asparagine	1031:1049	modified asparagine	1031:1049	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	9	66	gly	glycoprotein	1553:1564	arg1	glycoprotein					1553:1564	N-linked glycoprotein motifs	1544:1571	N-linked glycoprotein motifs	1544:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	4	67	gly	N-glycosylation	763:777	arg2	motifs					779:784	the canonical N-glycosylation motifs	749:784	the canonical N-glycosylation motifs	749:784	Notably, cysteines were highly enriched in the canonical N-glycosylation motifs N-!
35247785	6	68	theme	relative	1019:1026	arg1	position					1010:1017	the second position	999:1017	the second position relative to modified asparagine	999:1049	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	0	69	from	motif	68:72	arg1	enriched					19:26	enriched	19:26	enriched	19:26	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	7	70	gly	N-glycoproteins	1167:1181	arg1	N-glycoproteins					1167:1181	120 cysteine enriched N-glycoproteins	1145:1181	120 cysteine enriched N-glycoproteins	1145:1181	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	3	71	theme	cellular	599:606	arg1	annotation					618:627	cellular component annotation	599:627	cellular component annotation	599:627	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	6	72	theme	second	1003:1008	arg1	position					1010:1017	the second position	999:1017	the second position relative to modified asparagine	999:1049	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	8	73	theme	motif-containing	1371:1386	arg1	glycoproteins					1388:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	3	74	theme	glycoproteins	647:659	arg1	annotation					618:627	cellular component annotation	599:627	cellular component annotation	599:627	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	3	74	theme	glycoproteins	647:659	arg1	enrichment					633:642	enrichment	633:642	enrichment	633:642	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	9	75	theme	extraordinary	1615:1627	arg1	roles					1629:1633	extraordinary roles	1615:1633	extraordinary roles	1615:1633	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	4	76	theme	N-glycosylation	763:777	arg1	motifs					779:784	the canonical N-glycosylation motifs	749:784	the canonical N-glycosylation motifs	749:784	Notably, cysteines were highly enriched in the canonical N-glycosylation motifs N-!
35247785	2	77	theme	first	333:337	arg1	glycoproteome					361:373	the first bovine sperm N-linked glycoproteome	329:373	the first bovine sperm N-linked glycoproteome	329:373	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	7	78	theme	bovine	1312:1317	arg1	glycoproteome					1325:1337	the whole bovine sperm glycoproteome	1302:1337	the whole bovine sperm glycoproteome	1302:1337	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	0	79	from	enriched	19:26	arg1	motif					68:72	the canonical N-linked glycosylation motif	31:72	the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome	31:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	11	80	theme	N-glycosylation	1876:1890	arg1	mapping					1865:1871	The mapping	1861:1871	The mapping of N-glycosylation on bovine sperm	1861:1906	The mapping of N-glycosylation on bovine sperm may provide a new means to explore potential biomarkers for improving sperm quality and fertility.
35247785	0	81	theme	bovine	77:82	arg1	N-Glycoproteome					96:110	bovine spermatozoa N-Glycoproteome	77:110	bovine spermatozoa N-Glycoproteome	77:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	8	82	gly	glycoproteins	1388:1400	arg1	glycoproteins					1388:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	58 N-C-[S/T] motif-containing glycoproteins	1358:1400	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	5	83	theme	conservative	807:818	arg1	N-C-[S/T					827:834	the conservative motifs N-C-[S/T	803:834	the conservative motifs N-C-[S/T	803:834	P-[S/T/C] and the conservative motifs N-C-[S/T] were also significantly enriched, indicating these modifications play extraordinary roles in bovine spermatogenesis and maturation.
35247785	1	84	theme	post-translational	154:171	arg1	modifications					173:185	the most important post-translational modifications	135:185	the most important post-translational modifications of proteins	135:197	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	11	85	from	mapping	1865:1871	arg1	sperm					1902:1906	bovine sperm	1895:1906	bovine sperm	1895:1906	The mapping of N-glycosylation on bovine sperm may provide a new means to explore potential biomarkers for improving sperm quality and fertility.
35247785	8	86	theme	metalloproteinase	1424:1440	arg1	ADAM					1443:1446	metalloproteinase (ADAM)	1424:1447	metalloproteinase (ADAM)	1424:1447	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	0	87	theme	N-Glycoproteome	96:110	arg1	motif					68:72	the canonical N-linked glycosylation motif	31:72	the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome	31:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	0	88	gly	glycosylation	54:66	arg2	motif					68:72	the canonical N-linked glycosylation motif	31:72	the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome	31:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	7	89	theme	ion	1276:1278	arg1	binding					1280:1286	metal ion binding	1270:1286	metal ion binding	1270:1286	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	9	90	theme	key	1580:1582	arg1	cysteine-switches					1584:1600	key cysteine-switches	1580:1600	key cysteine-switches	1580:1600	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	8	91	theme	protein	1449:1455	arg1	domain					1457:1462	protein domain	1449:1462	protein domain	1449:1462	Strikingly, 15 of 58 N-C-[S/T] motif-containing glycoproteins had a disintegrin and metalloproteinase (ADAM) protein domain.
35247785	2	92	theme	1188 N-glycosylated	390:408	arg1	sites					410:414	1188 N-glycosylated sites	390:414	1188 N-glycosylated sites on 626 proteins	390:430	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	9	93	link	N-linked	1544:1551	arg1	motifs					1566:1571	N-linked glycoprotein motifs	1544:1571	N-linked glycoprotein motifs	1544:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	4	94	theme	N-	786:787	arg1	!					788:788	N-!	786:788	N-!	786:788	Notably, cysteines were highly enriched in the canonical N-glycosylation motifs N-!
35247785	7	95	theme	metalloendopeptidase	1236:1255	arg1	activity					1257:1264	significantly upregulated metalloendopeptidase activity	1210:1264	significantly upregulated metalloendopeptidase activity	1210:1264	A total of 120 cysteine enriched N-glycoproteins were identified, which had significantly upregulated metalloendopeptidase activity and metal ion binding compared with the whole bovine sperm glycoproteome.
35247785	9	96	from	residues	1532:1539	arg1	motifs					1566:1571	N-linked glycoprotein motifs	1544:1571	N-linked glycoprotein motifs	1544:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	6	97	theme	N-linked	1110:1117	arg1	glycoproteome					1119:1131	the previously reported N-linked glycoproteome	1086:1131	the previously reported N-linked glycoproteome	1086:1131	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	3	98	theme	reproductive	682:693	arg1	functions					695:703	proteolytic and reproductive functions	666:703	proteolytic and reproductive functions	666:703	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	3	99	gly	N-glycosylated	500:513	arg1	proteins					515:522	bovine sperm N-glycosylated proteins	487:522	bovine sperm N-glycosylated proteins	487:522	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	3	100	theme	sperm	494:498	arg1	proteins					515:522	bovine sperm N-glycosylated proteins	487:522	bovine sperm N-glycosylated proteins	487:522	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	10	101	contain	have	1711:1714	arg2	activity					1723:1730	enzyme activity	1716:1730	enzyme activity	1716:1730	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	10	101	contain	have	1711:1714	arg1	glycoproteins					1697:1709	almost all bovine sperm glycoproteins	1673:1709	almost all bovine sperm glycoproteins	1673:1709	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	3	102	theme	Bioinformatics	449:462	arg1	analysis					464:471	Bioinformatics analysis	449:471	Bioinformatics analysis	449:471	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	3	103	theme	proteolytic	666:676	arg1	functions					695:703	proteolytic and reproductive functions	666:703	proteolytic and reproductive functions	666:703	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	9	104	theme	free	1518:1521	arg1	residues					1532:1539	ADAM-containing conserved free cysteine residues	1492:1539	ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs	1492:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
35247785	0	105	theme	N-linked	45:52	arg1	motif					68:72	the canonical N-linked glycosylation motif	31:72	the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome	31:110	Cysteine is highly enriched in the canonical N-linked glycosylation motif of bovine spermatozoa N-Glycoproteome.
35247785	2	106	from	total	381:385	arg1	proteins					423:430	626 proteins	419:430	626 proteins	419:430	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	2	107	theme	bovine	339:344	arg1	glycoproteome					361:373	the first bovine sperm N-linked glycoproteome	329:373	the first bovine sperm N-linked glycoproteome	329:373	Herein, we mapped the first bovine sperm N-linked glycoproteome and a total of 1188 N-glycosylated sites on 626 proteins were identified.
35247785	4	108	from	motifs	779:784	arg1	enriched					737:744	enriched	737:744	enriched	737:744	Notably, cysteines were highly enriched in the canonical N-glycosylation motifs N-!
35247785	6	109	from	position	1010:1017	arg1	%					1058:1058	7.5%	1055:1058	7.5%	1055:1058	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	6	109	from	position	1010:1017	arg1	percentage					973:982	The percentage	969:982	The percentage of cysteine at the second position relative to modified asparagine	969:1049	The percentage of cysteine at the second position relative to modified asparagine was 7.5%, much higher than that of the previously reported N-linked glycoproteome.
35247785	1	110	theme	whole	235:239	arg1	process					241:247	the whole process	231:247	the whole process of spermatogenesis, sperm-egg recognition, and fertilization	231:308	Glycosylation, one of the most important post-translational modifications of proteins, plays an irreplaceable role in the whole process of spermatogenesis, sperm-egg recognition, and fertilization.
35247785	10	111	theme	indispensable	1770:1782	arg1	roles					1784:1788	indispensable roles	1770:1788	indispensable roles	1770:1788	In conclusion, almost all bovine sperm glycoproteins have enzyme activity, participate in proteolysis, and play indispensable roles in spermatogenesis, sperm-egg recognition, and eventual fertilization.
35247785	3	112	theme	region	560:565	arg1	"					566:566	"extracellular region"	545:566	"extracellular region"	545:566	Bioinformatics analysis revealed that bovine sperm N-glycosylated proteins were classified into "extracellular region" and "lysosome" groups based on cellular component annotation and enrichment of glycoproteins with proteolytic and reproductive functions.
35247785	9	113	theme	conserved	1508:1516	arg1	residues					1532:1539	ADAM-containing conserved free cysteine residues	1492:1539	ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs	1492:1571	Thus, we hypothesized that ADAM-containing conserved free cysteine residues in N-linked glycoprotein motifs may be key cysteine-switches and may have extraordinary roles in bovine spermatozoa.
37280111	4	0	dep	higher	1423:1428	arg1	<					1433:1433	P < 0.05	1431:1438	P < 0.05	1431:1438	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	0	1	from	performance	34:44	arg1	community					141:149	bacterial community	131:149	bacterial community	131:149	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	0	1	from	performance	34:44	arg1	profiles					167:174	functional profiles	156:174	functional profiles	156:174	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	0	1	from	performance	34:44	arg1	fermentation					117:128	alfalfa silage fermentation	102:128	alfalfa silage fermentation	102:128	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	5	2	theme	gene-predicted	1729:1742	arg1	analyses					1755:1762	gene-predicted functional analyses	1729:1762	the 16S rRNA gene-predicted functional analyses	1716:1762	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	6	3	theme	various	2439:2445	arg1	kinds					2447:2451	various kinds	2439:2451	various kinds of silages	2439:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	6	4	theme	dominant	2069:2076	arg1	species					2082:2088	dominant LAB species	2069:2088	dominant LAB species	2069:2088	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	2	5	theme	colony	771:776	arg1	cfu					793:795	cfu	793:795	cfu	793:795	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	5	theme	colony	771:776	arg1	units					786:790	1.0 × 109 colony forming units	761:790	1.0 × 109 colony forming units (cfu) per kilogram	761:809	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	6	6	theme	d	2255:2255	arg1	ensiling					2257:2264	60 d ensiling	2252:2264	60 d ensiling	2252:2264	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	7	dep	higher	1036:1041	arg1	<					1046:1046	P < 0.05	1044:1051	P < 0.05	1044:1051	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	1	8	theme	animal	254:259	arg1	feed					261:264	an animal feed	251:264	an animal feed	251:264	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	3	9	from	pH	1029:1030	arg1	Lb-					1154:1156	Lb-	1154:1156	Lb-	1154:1156	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	9	from	pH	1029:1030	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	9	from	pH	1029:1030	arg1	LbPp-					1159:1163	LbPp-	1159:1163	LbPp-	1159:1163	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	6	10	from	inoculants	2425:2434	arg1	kinds					2447:2451	various kinds	2439:2451	various kinds of silages	2439:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	2	11	theme	buchneri	697:704	arg1	effects					570:576	the effects	566:576	the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	566:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	3	12	theme	organic	1065:1071	arg1	contents					1078:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	4	13	theme	9.92	1452:1455	arg1	count					1445:1449	higher (P < 0.05) LAB count	1423:1449	higher (P < 0.05) LAB count (9.92 log10 cfu/g)	1423:1468	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	13	theme	9.92	1452:1455	arg1	cfu/g					1463:1467	9.92 log10 cfu/g	1452:1467	9.92 log10 cfu/g	1452:1467	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	2	14	theme	homofermentative	581:596	arg1	Pp					661:662	Pp	661:662	Pp	661:662	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	14	theme	homofermentative	581:596	arg1	LAB					598:600	homofermentative LAB	581:600	homofermentative LAB	581:600	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	6	15	theme	molds	2131:2135	arg1	growth					2109:2114	the growth	2105:2114	the growth of Clostridia, molds, and yeasts	2105:2147	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	6	16	with	combination	2052:2062	arg1	species					2082:2088	dominant LAB species	2069:2088	dominant LAB species	2069:2088	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	4	17	theme	positive	1497:1504	arg1	correlation					1506:1516	a positive correlation	1495:1516	a positive correlation	1495:1516	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	1	18	theme	lactic	432:437	arg1	LAB					454:456	LAB	454:456	LAB	454:456	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	1	18	theme	lactic	432:437	arg1	bacteria					444:451	lactic acid bacteria	432:451	lactic acid bacteria (LAB)	432:457	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	6	19	theme	Clostridia	2119:2128	arg1	growth					2109:2114	the growth	2105:2114	the growth of Clostridia, molds, and yeasts	2105:2147	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	5	20	from	degradation	1901:1911	arg1	alfalfa					1935:1941	alfalfa	1935:1941	alfalfa	1935:1941	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	1	21	theme	high	345:348	arg1	content					356:362	high water content	345:362	high water content	345:362	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	3	22	theme	P	1235:1235	arg1	<					1237:1237	P < 0.05	1235:1242	P < 0.05	1235:1242	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	6	23	theme	further	2296:2302	arg1	studies					2304:2310	further studies	2296:2310	further studies	2296:2310	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	24	theme	g/kg	1363:1366	arg1	DM					1368:1369	10.92 g/kg DM	1357:1369	10.92 g/kg DM	1357:1369	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	24	theme	g/kg	1363:1366	arg1	d					1354:1354	60 d	1351:1354	60 d (10.92 g/kg DM)	1351:1370	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	25	from	contents	1078:1085	arg1	Lb-					1154:1156	Lb-	1154:1156	Lb-	1154:1156	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	25	from	contents	1078:1085	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	25	from	contents	1078:1085	arg1	LbPp-					1159:1163	LbPp-	1159:1163	LbPp-	1159:1163	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	26	theme	higher	1227:1232	arg1	contents					1249:1256	higher (P < 0.05) WSC contents	1227:1256	higher (P < 0.05) WSC contents	1227:1256	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	5	27	theme	further	1893:1899	arg1	degradation					1901:1911	further degradation	1893:1911	further degradation of polysaccharides in alfalfa	1893:1941	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	2	28	theme	alfalfa	915:921	arg1	silage					923:928	alfalfa silage	915:928	alfalfa silage after 7, 14, 30, and 60 ensiling days	915:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	3	29	from	xylose	1088:1093	arg1	Lb-					1154:1156	Lb-	1154:1156	Lb-	1154:1156	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	29	from	xylose	1088:1093	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	29	from	xylose	1088:1093	arg1	LbPp-					1159:1163	LbPp-	1159:1163	LbPp-	1159:1163	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	2	30	theme	metagenomic	524:534	arg1	technology					545:554	high-throughput metagenomic sequence technology	508:554	high-throughput metagenomic sequence technology	508:554	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	3	31	theme	WSC	1245:1247	arg1	contents					1249:1256	higher (P < 0.05) WSC contents	1227:1256	higher (P < 0.05) WSC contents	1227:1256	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	2	32	theme	pentosaceus	648:658	arg1	effects					570:576	the effects	566:576	the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	566:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	3	33	theme	crude	1096:1100	arg1	protein					1102:1108	crude protein	1096:1108	crude protein	1096:1108	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	4	34	with	silages	1591:1597	arg1	properties					1673:1682	fermentation properties	1660:1682	fermentation properties after 30 and 60 d	1660:1700	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	1	35	theme	buffering	378:386	arg1	capacity					388:395	elevated buffering capacity	369:395	elevated buffering capacity	369:395	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	6	36	theme	significant	1993:2003	arg1	performance					2005:2015	the significant performance	1989:2015	the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species	1989:2088	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	37	dep	higher	1227:1232	arg1	<					1237:1237	P < 0.05	1235:1242	P < 0.05	1235:1242	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	2	38	theme	heterofermentative	670:687	arg1	LAB					689:691	heterofermentative LAB	670:691	heterofermentative LAB	670:691	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	4	39	theme	LbLp-inoculated	1567:1581	arg1	silages					1591:1597	LbLp-inoculated alfalfa silages	1567:1597	LbLp-inoculated alfalfa silages	1567:1597	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	39	theme	LbLp-inoculated	1567:1581	arg1	Lactobacillus					1624:1636	Lactobacillus	1624:1636	Lactobacillus	1624:1636	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	39	theme	LbLp-inoculated	1567:1581	arg1	Pediococcus					1642:1652	Pediococcus	1642:1652	Pediococcus	1642:1652	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	6	40	theme	combination	2358:2368	arg1	performance					2335:2345	the diverse performance	2323:2345	the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages	2323:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	0	41	theme	heterofermentative	59:76	arg1	bacteria					90:97	homo- and heterofermentative lactic acid bacteria	49:97	homo- and heterofermentative lactic acid bacteria	49:97	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	5	42	dep	L.	1788:1789	arg1	buchneri					1791:1798	buchneri	1791:1798	buchneri	1791:1798	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	4	43	theme	LbLp-inoculated	1382:1396	arg1	silages					1406:1412	LbLp-inoculated alfalfa silages	1382:1412	LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d.	1382:1480	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	0	44	theme	acid	85:88	arg1	bacteria					90:97	homo- and heterofermentative lactic acid bacteria	49:97	homo- and heterofermentative lactic acid bacteria	49:97	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	1	45	theme	water-soluble	311:323	arg1	WSC					339:341	WSC	339:341	WSC	339:341	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	1	45	theme	water-soluble	311:323	arg1	carbohydrate					325:336	its low water-soluble carbohydrate	303:336	its low water-soluble carbohydrate (WSC)	303:342	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	3	46	from	nitrogen	1119:1126	arg1	Lb-					1154:1156	Lb-	1154:1156	Lb-	1154:1156	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	46	from	nitrogen	1119:1126	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	46	from	nitrogen	1119:1126	arg1	LbPp-					1159:1163	LbPp-	1159:1163	LbPp-	1159:1163	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	2	47	theme	fresh	830:834	arg1	material					836:843	alfalfa biomass fresh material	814:843	alfalfa biomass fresh material	814:843	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	4	48	theme	combined	1540:1547	arg1	inoculants					1553:1562	the combined LAB inoculants	1536:1562	the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d	1536:1700	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	2	49	theme	Lactobacillus	603:615	arg1	effects					570:576	the effects	566:576	the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	566:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	50	theme	ensiling	954:961	arg1	days					963:966	7, 14, 30, and 60 ensiling days	936:966	7, 14, 30, and 60 ensiling days	936:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	0	51	theme	silage	110:115	arg1	fermentation					117:128	alfalfa silage fermentation	102:128	alfalfa silage fermentation	102:128	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	3	52	theme	alfalfa	1291:1297	arg1	silages					1299:1305	LbLp-inoculated alfalfa silages	1275:1305	LbLp-inoculated alfalfa silages	1275:1305	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	6	53	theme	artificial	2414:2423	arg1	inoculants					2425:2434	other natural and artificial inoculants	2396:2434	other natural and artificial inoculants in various kinds of silages	2396:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	0	54	theme	Metagenomics	0:11	arg1	analysis					13:20	Metagenomics analysis	0:20	Metagenomics analysis	0:20	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	5	55	dep	rRNA	1724:1727	arg1	analyses					1755:1762	gene-predicted functional analyses	1729:1762	the 16S rRNA gene-predicted functional analyses	1716:1762	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	6	56	theme	natural	2402:2408	arg1	inoculants					2425:2434	other natural and artificial inoculants	2396:2434	other natural and artificial inoculants in various kinds of silages	2396:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	5	57	theme	carbohydrate	1853:1864	arg1	metabolism					1866:1875	carbohydrate metabolism	1853:1875	carbohydrate metabolism	1853:1875	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	6	58	theme	buchneri	2023:2030	arg1	performance					2005:2015	the significant performance	1989:2015	the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species	1989:2088	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	5	59	theme	plantarum	1813:1821	arg1	combination					1832:1842	L. plantarum YC1-1-4B combination	1810:1842	L. plantarum YC1-1-4B combination	1810:1842	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	4	60	theme	LAB	1441:1443	arg1	count					1445:1449	higher (P < 0.05) LAB count	1423:1449	higher (P < 0.05) LAB count (9.92 log10 cfu/g)	1423:1468	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	60	theme	LAB	1441:1443	arg1	cfu/g					1463:1467	9.92 log10 cfu/g	1452:1467	9.92 log10 cfu/g	1452:1467	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	61	theme	dominant	1603:1610	arg1	genera					1616:1621	dominant LAB genera	1603:1621	dominant LAB genera	1603:1621	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	62	dep	count	1445:1449	arg1	d.					1479:1480	d.	1479:1480	d.	1479:1480	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	2	63	theme	biomass	822:828	arg1	material					836:843	alfalfa biomass fresh material	814:843	alfalfa biomass fresh material	814:843	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	4	64	theme	higher	1423:1428	arg1	count					1445:1449	higher (P < 0.05) LAB count	1423:1449	higher (P < 0.05) LAB count (9.92 log10 cfu/g)	1423:1468	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	64	theme	higher	1423:1428	arg1	cfu/g					1463:1467	9.92 log10 cfu/g	1452:1467	9.92 log10 cfu/g	1452:1467	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	6	65	theme	functional	2200:2209	arg1	metabolism					2224:2233	functional carbohydrate metabolism	2200:2233	functional carbohydrate metabolism	2200:2233	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	66	theme	alfalfa	1186:1192	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	0	67	theme	homo-	49:53	arg1	bacteria					90:97	homo- and heterofermentative lactic acid bacteria	49:97	homo- and heterofermentative lactic acid bacteria	49:97	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	3	68	from	reduction	993:1001	arg1	protein					1102:1108	crude protein	1096:1108	crude protein	1096:1108	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	68	from	reduction	993:1001	arg1	glucose					1017:1023	glucose	1017:1023	glucose	1017:1023	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	68	from	reduction	993:1001	arg1	stability					1141:1149	aerobic stability	1133:1149	aerobic stability	1133:1149	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	68	from	reduction	993:1001	arg1	contents					1078:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	68	from	reduction	993:1001	arg1	xylose					1088:1093	xylose	1088:1093	xylose	1088:1093	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	68	from	reduction	993:1001	arg1	pH					1029:1030	pH	1029:1030	pH	1029:1030	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	68	from	reduction	993:1001	arg1	nitrogen					1119:1126	ammonia nitrogen	1111:1126	ammonia nitrogen	1111:1126	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	69	theme	dry	1330:1332	arg1	d					1316:1316	30 d	1313:1316	30 d (10.84 g/kg dry matter [DM])	1313:1345	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	69	theme	dry	1330:1332	arg1	[DM					1341:1343	10.84 g/kg dry matter [DM	1319:1343	10.84 g/kg dry matter [DM	1319:1343	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	4	70	theme	P	1431:1431	arg1	<					1433:1433	P < 0.05	1431:1438	P < 0.05	1431:1438	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	6	71	with	consortium	2380:2389	arg1	inoculants					2425:2434	other natural and artificial inoculants	2396:2434	other natural and artificial inoculants in various kinds of silages	2396:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	6	72	from	performance	2005:2015	arg1	combination					2052:2062	combination	2052:2062	combination with dominant LAB species	2052:2088	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	2	73	theme	combinations	721:732	arg1	effects					570:576	the effects	566:576	the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	566:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	6	74	theme	silages	2456:2462	arg1	kinds					2447:2451	various kinds	2439:2451	various kinds of silages	2439:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	1	75	theme	roughage	219:226	arg1	kind					211:214	a kind	209:214	a kind of roughage frequently utilized as an animal feed	209:264	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	1	75	theme	roughage	219:226	arg1	Alfalfa					177:183	Alfalfa	177:183	Alfalfa (Medicago sativa L.)	177:204	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	6	76	theme	LAB	2354:2356	arg1	combination					2358:2368	the LAB combination	2350:2368	the LAB combination	2350:2368	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	6	77	theme	LAB	2078:2080	arg1	species					2082:2088	dominant LAB species	2069:2088	dominant LAB species	2069:2088	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	2	78	theme	alfalfa	814:820	arg1	material					836:843	alfalfa biomass fresh material	814:843	alfalfa biomass fresh material	814:843	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	5	79	theme	functional	1744:1753	arg1	analyses					1755:1762	gene-predicted functional analyses	1729:1762	the 16S rRNA gene-predicted functional analyses	1716:1762	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	2	80	theme	forming	778:784	arg1	cfu					793:795	cfu	793:795	cfu	793:795	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	80	theme	forming	778:784	arg1	units					786:790	1.0 × 109 colony forming units	761:790	1.0 × 109 colony forming units (cfu) per kilogram	761:809	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	1	81	dep	Alfalfa	177:183	arg1	L.					202:203	Medicago sativa L.	186:203	Medicago sativa L.	186:203	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	6	82	theme	yeasts	2142:2147	arg1	growth					2109:2114	the growth	2105:2114	the growth of Clostridia, molds, and yeasts	2105:2147	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	5	83	theme	L.	1810:1811	arg1	combination					1832:1842	L. plantarum YC1-1-4B combination	1810:1842	L. plantarum YC1-1-4B combination	1810:1842	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	4	84	theme	log10	1457:1461	arg1	count					1445:1449	higher (P < 0.05) LAB count	1423:1449	higher (P < 0.05) LAB count (9.92 log10 cfu/g)	1423:1468	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	84	theme	log10	1457:1461	arg1	cfu/g					1463:1467	9.92 log10 cfu/g	1452:1467	9.92 log10 cfu/g	1452:1467	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	2	85	theme	LAB	598:600	arg1	effects					570:576	the effects	566:576	the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	566:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	5	86	theme	YC1-1-4B	1823:1830	arg1	combination					1832:1842	L. plantarum YC1-1-4B combination	1810:1842	L. plantarum YC1-1-4B combination	1810:1842	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	3	87	theme	acid	1073:1076	arg1	contents					1078:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	6	88	theme	alfalfa	2238:2244	arg1	characteristics					2180:2194	fermentation characteristics	2167:2194	fermentation characteristics	2167:2194	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	6	88	theme	alfalfa	2238:2244	arg1	metabolism					2224:2233	functional carbohydrate metabolism	2200:2233	functional carbohydrate metabolism	2200:2233	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	89	theme	higher	1036:1041	arg1	contents					1078:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	5	90	theme	L.	1788:1789	arg1	PC-C1					1800:1804	the L. buchneri PC-C1	1784:1804	the L. buchneri PC-C1	1784:1804	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	3	91	theme	beneficial	1054:1063	arg1	contents					1078:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	higher (P < 0.05) beneficial organic acid contents	1036:1085	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	0	92	theme	bacterial	131:139	arg1	community					141:149	bacterial community	131:149	bacterial community	131:149	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	1	93	theme	acid	439:442	arg1	LAB					454:456	LAB	454:456	LAB	454:456	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	1	93	theme	acid	439:442	arg1	bacteria					444:451	lactic acid bacteria	432:451	lactic acid bacteria (LAB)	432:457	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	2	94	theme	functional	892:901	arg1	profiles					903:910	functional profiles	892:910	functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	892:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	1	95	theme	water	350:354	arg1	content					356:362	high water content	345:362	high water content	345:362	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	6	96	with	combination	2358:2368	arg1	inoculants					2425:2434	other natural and artificial inoculants	2396:2434	other natural and artificial inoculants in various kinds of silages	2396:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	0	97	theme	functional	156:165	arg1	profiles					167:174	functional profiles	156:174	functional profiles	156:174	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	3	98	theme	P	1044:1044	arg1	<					1046:1046	P < 0.05	1044:1051	P < 0.05	1044:1051	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	2	99	theme	sequence	536:543	arg1	technology					545:554	high-throughput metagenomic sequence technology	508:554	high-throughput metagenomic sequence technology	508:554	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	100	theme	silage	923:928	arg1	fermentation					853:864	the fermentation	849:864	the fermentation	849:864	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	100	theme	silage	923:928	arg1	community					877:885	microbial community	867:885	microbial community	867:885	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	100	theme	silage	923:928	arg1	profiles					903:910	functional profiles	892:910	functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	892:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	3	101	theme	ammonia	1111:1117	arg1	nitrogen					1119:1126	ammonia nitrogen	1111:1126	ammonia nitrogen	1111:1126	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	1	102	theme	elevated	369:376	arg1	capacity					388:395	elevated buffering capacity	369:395	elevated buffering capacity	369:395	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	2	103	theme	high-throughput	508:522	arg1	technology					545:554	high-throughput metagenomic sequence technology	508:554	high-throughput metagenomic sequence technology	508:554	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	104	theme	LAB	689:691	arg1	effects					570:576	the effects	566:576	the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	566:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	4	105	theme	alfalfa	1583:1589	arg1	silages					1591:1597	LbLp-inoculated alfalfa silages	1567:1597	LbLp-inoculated alfalfa silages	1567:1597	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	105	theme	alfalfa	1583:1589	arg1	Lactobacillus					1624:1636	Lactobacillus	1624:1636	Lactobacillus	1624:1636	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	105	theme	alfalfa	1583:1589	arg1	Pediococcus					1642:1652	Pediococcus	1642:1652	Pediococcus	1642:1652	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	0	106	theme	lactic	78:83	arg1	bacteria					90:97	homo- and heterofermentative lactic acid bacteria	49:97	homo- and heterofermentative lactic acid bacteria	49:97	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	3	107	theme	aerobic	1133:1139	arg1	stability					1141:1149	aerobic stability	1133:1149	aerobic stability	1133:1149	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	5	108	theme	16S	1720:1722	arg1	rRNA					1724:1727	the 16S rRNA gene-predicted functional analyses	1716:1762	the 16S rRNA gene-predicted functional analyses	1716:1762	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	0	109	theme	bacteria	90:97	arg1	performance					34:44	the performance	30:44	the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles	30:174	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	1	110	theme	low	307:309	arg1	WSC					339:341	WSC	339:341	WSC	339:341	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	1	110	theme	low	307:309	arg1	carbohydrate					325:336	its low water-soluble carbohydrate	303:336	its low water-soluble carbohydrate (WSC)	303:342	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	2	111	dep	Lactobacillus	603:615	arg1	plantarum					617:625	Lactobacillus plantarum (Lp)	603:630	Lactobacillus plantarum (Lp)	603:630	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	4	112	theme	LAB	1549:1551	arg1	inoculants					1553:1562	the combined LAB inoculants	1536:1562	the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d	1536:1700	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	0	113	theme	alfalfa	102:108	arg1	fermentation					117:128	alfalfa silage fermentation	102:128	alfalfa silage fermentation	102:128	Metagenomics analysis reveals the performance of homo- and heterofermentative lactic acid bacteria in alfalfa silage fermentation, bacterial community, and functional profiles.
37280111	2	114	theme	microbial	867:875	arg1	community					877:885	microbial community	867:885	microbial community	867:885	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	4	115	theme	alfalfa	1398:1404	arg1	silages					1406:1412	LbLp-inoculated alfalfa silages	1382:1412	LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d.	1382:1480	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	6	116	theme	diverse	2327:2333	arg1	performance					2335:2345	the diverse performance	2323:2345	the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages	2323:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	117	theme	LbLp-inoculated	1275:1289	arg1	silages					1299:1305	LbLp-inoculated alfalfa silages	1275:1305	LbLp-inoculated alfalfa silages	1275:1305	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	2	118	theme	Pediococcus	636:646	arg1	pentosaceus					648:658	Pediococcus pentosaceus	636:658	Pediococcus pentosaceus	636:658	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	3	119	from	protein	1102:1108	arg1	Lb-					1154:1156	Lb-	1154:1156	Lb-	1154:1156	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	119	from	protein	1102:1108	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	119	from	protein	1102:1108	arg1	LbPp-					1159:1163	LbPp-	1159:1163	LbPp-	1159:1163	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	6	120	theme	plantarum	2039:2047	arg1	performance					2005:2015	the significant performance	1989:2015	the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species	1989:2088	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	121	theme	LbLp-inoculated	1170:1184	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	5	122	theme	ensiling	1957:1964	arg1	d					1952:1952	60 d	1949:1952	60 d of ensiling	1949:1964	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	2	123	from	effects	570:576	arg1	fermentation					853:864	the fermentation	849:864	the fermentation	849:864	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	123	from	effects	570:576	arg1	community					877:885	microbial community	867:885	microbial community	867:885	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	123	from	effects	570:576	arg1	profiles					903:910	functional profiles	892:910	functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days	892:966	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	4	124	theme	LAB	1612:1614	arg1	genera					1616:1621	dominant LAB genera	1603:1621	dominant LAB genera	1603:1621	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	6	125	theme	other	2396:2400	arg1	inoculants					2425:2434	other natural and artificial inoculants	2396:2434	other natural and artificial inoculants in various kinds of silages	2396:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	6	126	dep	characteristics	2180:2194	arg1	the					2163:2165	the	2163:2165	the	2163:2165	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	127	dep	d.	1218:1219	arg1	recorded					1263:1270	recorded	1263:1270	were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM)	1258:1370	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	128	theme	matter	1334:1339	arg1	d					1316:1316	30 d	1313:1316	30 d (10.84 g/kg dry matter [DM])	1313:1345	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	128	theme	matter	1334:1339	arg1	[DM					1341:1343	10.84 g/kg dry matter [DM	1319:1343	10.84 g/kg dry matter [DM	1319:1343	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	6	129	theme	carbohydrate	2211:2222	arg1	metabolism					2224:2233	functional carbohydrate metabolism	2200:2233	functional carbohydrate metabolism	2200:2233	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	1	130	theme	bacteria	444:451	arg1	application					417:427	the application	413:427	the application of lactic acid bacteria (LAB) to improve its fermentation	413:485	Alfalfa (Medicago sativa L.) is a kind of roughage frequently utilized as an animal feed but challenging to be ensiled due to its low water-soluble carbohydrate (WSC), high water content, and elevated buffering capacity, thus requiring the application of lactic acid bacteria (LAB) to improve its fermentation.
37280111	6	131	theme	consortium	2380:2389	arg1	performance					2335:2345	the diverse performance	2323:2345	the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages	2323:2462	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	3	132	from	glucose	1017:1023	arg1	Lb-					1154:1156	Lb-	1154:1156	Lb-	1154:1156	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	132	from	glucose	1017:1023	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	132	from	glucose	1017:1023	arg1	LbPp-					1159:1163	LbPp-	1159:1163	LbPp-	1159:1163	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	4	133	with	genera	1616:1621	arg1	properties					1673:1682	fermentation properties	1660:1682	fermentation properties after 30 and 60 d	1660:1700	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	3	134	theme	g/kg	1325:1328	arg1	d					1316:1316	30 d	1313:1316	30 d (10.84 g/kg dry matter [DM])	1313:1345	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	134	theme	g/kg	1325:1328	arg1	[DM					1341:1343	10.84 g/kg dry matter [DM	1319:1343	10.84 g/kg dry matter [DM	1319:1343	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	5	135	theme	polysaccharides	1916:1930	arg1	degradation					1901:1911	further degradation	1893:1911	further degradation of polysaccharides in alfalfa	1893:1941	In addition, the 16S rRNA gene-predicted functional analyses further showed that the L. buchneri PC-C1 and L. plantarum YC1-1-4B combination improved carbohydrate metabolism and facilitated further degradation of polysaccharides in alfalfa after 60 d of ensiling.
37280111	2	136	theme	×	765:765	arg1	cfu					793:795	cfu	793:795	cfu	793:795	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	2	136	theme	×	765:765	arg1	units					786:790	1.0 × 109 colony forming units	761:790	1.0 × 109 colony forming units (cfu) per kilogram	761:809	This study employed high-throughput metagenomic sequence technology to reveal the effects of homofermentative LAB, Lactobacillus plantarum (Lp), or Pediococcus pentosaceus (Pp), and heterofermentative LAB, L. buchneri (Lb), or their combinations (LbLp or LbPp) (applied at 1.0 × 109 colony forming units (cfu) per kilogram of alfalfa biomass fresh material) on the fermentation, microbial community, and functional profiles of alfalfa silage after 7, 14, 30, and 60 ensiling days.
37280111	6	137	theme	fermentation	2167:2178	arg1	characteristics					2180:2194	fermentation characteristics	2167:2194	fermentation characteristics	2167:2194	These findings reveal the significant performance of L. buchneri and L. plantarum in combination with dominant LAB species in suppressing the growth of Clostridia, molds, and yeasts and improving the fermentation characteristics and functional carbohydrate metabolism of alfalfa after 60 d ensiling, thus suggesting the need for further studies to uncover the diverse performance of the LAB combination and their consortium with other natural and artificial inoculants in various kinds of silages.
37280111	4	138	from	inoculants	1553:1562	arg1	silages					1591:1597	LbLp-inoculated alfalfa silages	1567:1597	LbLp-inoculated alfalfa silages	1567:1597	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	138	from	inoculants	1553:1562	arg1	Lactobacillus					1624:1636	Lactobacillus	1624:1636	Lactobacillus	1624:1636	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	138	from	inoculants	1553:1562	arg1	Pediococcus					1642:1652	Pediococcus	1642:1652	Pediococcus	1642:1652	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	4	138	from	inoculants	1553:1562	arg1	genera					1616:1621	dominant LAB genera	1603:1621	dominant LAB genera	1603:1621	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	3	139	theme	P	1004:1004	arg1	reduction					993:1001	a reduction	991:1001	a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages	991:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	139	theme	P	1004:1004	arg1	<					1006:1006	P < 0.05	1004:1011	P < 0.05	1004:1011	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	4	140	theme	fermentation	1660:1671	arg1	properties					1673:1682	fermentation properties	1660:1682	fermentation properties after 30 and 60 d	1660:1700	Besides, LbLp-inoculated alfalfa silages recorded higher (P < 0.05) LAB count (9.92 log10 cfu/g) after 60 d. Furthermore, a positive correlation was found between the combined LAB inoculants in LbLp-inoculated alfalfa silages and dominant LAB genera, Lactobacillus and Pediococcus, with fermentation properties after 30 and 60 d.
37280111	3	141	from	stability	1141:1149	arg1	Lb-					1154:1156	Lb-	1154:1156	Lb-	1154:1156	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	141	from	stability	1141:1149	arg1	silages					1194:1200	LbLp-inoculated alfalfa silages	1170:1200	LbLp-inoculated alfalfa silages	1170:1200	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
37280111	3	141	from	stability	1141:1149	arg1	LbPp-					1159:1163	LbPp-	1159:1163	LbPp-	1159:1163	The results indicated a reduction (P < 0.05) in glucose and pH and higher (P < 0.05) beneficial organic acid contents, xylose, crude protein, ammonia nitrogen, and aerobic stability in Lb-, LbPp-, and LbLp-inoculated alfalfa silages after 30 and 60 d. Also, higher (P < 0.05) WSC contents were recorded in LbLp-inoculated alfalfa silages after 30 d (10.84 g/kg dry matter [DM]) and 60 d (10.92 g/kg DM).
36619394	0	0	theme	pH-controlled	66:78	arg1	kinetics					80:87	a pH-controlled kinetics	64:87	a pH-controlled kinetics	64:87	Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics.
36619394	4	1	theme	cell	832:835	arg1	populations					837:847	the major brain cell populations	816:847	the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes)	816:915	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	1	2	theme	cell	139:142	arg1	models					144:149	cell models	139:149	cell models representative of neural cells	139:180	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	1	3	theme	viscoelastic	219:230	arg1	properties					232:241	the basic viscoelastic properties	209:241	the basic viscoelastic properties of brain tissue	209:257	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	4	4	theme	brain	826:830	arg1	populations					837:847	the major brain cell populations	816:847	the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes)	816:915	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	6	5	theme	multiple	1236:1243	arg1	kinetics					1273:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	8	6	theme	pectin-based	1533:1544	arg1	hydrogels					1546:1554	pectin-based hydrogels	1533:1554	pectin-based hydrogels	1533:1554	Taken together, the results suggest that pectin-based hydrogels could be successfully applied for neural cell culture.
36619394	4	7	theme	populations	837:847	arg1	representative					798:811	representative	798:811	representative	798:811	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	2	8	theme	ink	337:339	arg1	printability					341:352	ink printability	337:352	ink printability	337:352	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	1	9	theme	brain	246:250	arg1	tissue					252:257	brain tissue	246:257	brain tissue	246:257	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	1	10	with	view	111:114	arg1	models					144:149	cell models	139:149	cell models representative of neural cells	139:180	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	5	11	link	crosslinked	1065:1075	arg1	polysaccharides					1077:1091	internally crosslinked polysaccharides	1054:1091	internally crosslinked polysaccharides	1054:1091	Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments.
36619394	1	12	theme	representative	151:164	arg1	models					144:149	cell models	139:149	cell models representative of neural cells	139:180	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	0	13	theme	neural	38:43	arg1	tissue					45:50	neural tissue	38:50	neural tissue	38:50	Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics.
36619394	4	14	theme	representative	798:811	arg1	models					791:796	Different cell models	776:796	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes)	776:915	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	0	15	theme	Pectin-based	0:11	arg1	bioinks					13:19	Pectin-based bioinks	0:19	Pectin-based bioinks for 3D models of neural tissue	0:50	Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics.
36619394	6	16	contain	have	1231:1234	arg1	it					1213:1214	it	1213:1214	it	1213:1214	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	6	16	contain	have	1231:1234	arg2	kinetics					1273:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	2	17	theme	3D-reactive	429:439	arg1	printing					441:448	the previously published 3D-reactive printing	404:448	the previously published 3D-reactive printing	404:448	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	5	18	theme	crosslinked	1065:1075	arg1	polysaccharides					1077:1091	internally crosslinked polysaccharides	1054:1091	internally crosslinked polysaccharides	1054:1091	Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments.
36619394	4	19	theme	major	820:824	arg1	populations					837:847	the major brain cell populations	816:847	the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes)	816:915	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	3	20	theme	cell	726:729	arg1	medium					739:744	cell culture medium	726:744	cell culture medium	726:744	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	1	21	theme	neural	169:174	arg1	cells					176:180	neural cells	169:180	neural cells	169:180	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	2	22	theme	published	419:427	arg1	printing					441:448	the previously published 3D-reactive printing	404:448	the previously published 3D-reactive printing	404:448	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	1	23	theme	tissue	252:257	arg1	properties					232:241	the basic viscoelastic properties	209:241	the basic viscoelastic properties of brain tissue	209:257	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	7	24	theme	hydrogel	1467:1474	arg1	microstructure					1476:1489	hydrogel microstructure	1467:1489	hydrogel microstructure	1467:1489	In addition, the results indicate that not only cells survive and proliferate following 3D-bioprinting, but they can also interact and reorganize hydrogel microstructure.
36619394	1	25	theme	cells	176:180	arg1	representative					151:164	representative	151:164	representative	151:164	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	0	26	theme	3D	25:26	arg1	models					28:33	3D models	25:33	3D models of neural tissue	25:50	Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics.
36619394	3	27	theme	culture	731:737	arg1	medium					739:744	cell culture medium	726:744	cell culture medium	726:744	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	2	28	theme	printing	528:535	arg1	time					537:540	printing time	528:540	printing time	528:540	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	4	29	theme	cell	786:789	arg1	models					791:796	Different cell models	776:796	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes)	776:915	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	3	30	theme	gels	624:627	arg1	properties					569:578	The viscoelastic properties	552:578	The viscoelastic properties	552:578	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	3	30	theme	gels	624:627	arg1	printability					581:592	printability	581:592	printability	581:592	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	3	30	theme	gels	624:627	arg1	microstructure					599:612	microstructure	599:612	microstructure	599:612	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	8	31	theme	cell	1597:1600	arg1	culture					1602:1608	neural cell culture	1590:1608	neural cell culture	1590:1608	Taken together, the results suggest that pectin-based hydrogels could be successfully applied for neural cell culture.
36619394	4	32	theme	Different	776:784	arg1	models					791:796	Different cell models	776:796	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes)	776:915	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	4	33	dep	populations	837:847	arg1	neurons					856:862	neurons	856:862	neurons	856:862	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	4	33	dep	populations	837:847	arg1	oligodendrocytes					899:914	oligodendrocytes	899:914	oligodendrocytes	899:914	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	4	33	dep	populations	837:847	arg1	astrocytes					865:874	astrocytes	865:874	astrocytes	865:874	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	4	33	dep	populations	837:847	arg1	cells					888:892	microglial cells	877:892	microglial cells	877:892	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	3	34	theme	HEPES	747:751	arg1	buffer					753:758	HEPES buffer	747:758	HEPES buffer	747:758	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	4	35	theme	microglial	877:886	arg1	neurons					856:862	neurons	856:862	neurons	856:862	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	4	35	theme	microglial	877:886	arg1	cells					888:892	microglial cells	877:892	microglial cells	877:892	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	2	36	dep	expands	379:385	arg1	approach					450:457	approach	450:457	approach by introducing pH as a key parameter to be controlled, together with printing time	450:540	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	6	37	theme	hydrogel	1299:1306	arg1	composition					1308:1318	hydrogel composition	1299:1318	hydrogel composition	1299:1318	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	0	38	theme	tissue	45:50	arg1	models					28:33	3D models	25:33	3D models of neural tissue	25:50	Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics.
36619394	3	39	dep	optimized	696:704	arg1	i.e.					707:710	i.e.	707:710	i.e.	707:710	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	6	40	theme	crosslinking	1260:1271	arg1	kinetics					1273:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	3	41	theme	pectin	617:622	arg1	gels					624:627	pectin gels	617:627	pectin gels crosslinked with CaCO3	617:650	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	2	42	dep	concept	276:282	arg1	provides					298:305	provides	298:305	provides useful information to predict ink printability	298:352	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	2	43	theme	printing	441:448	arg1	potential					391:399	the potential	387:399	the potential of the previously published 3D-reactive printing	387:448	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	3	44	dep	i.e.	707:710	arg1	including					716:724	including	716:724	including cell culture medium, HEPES buffer, and collagen	716:772	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	5	45	theme	study	979:983	arg1	outcomes					962:969	The outcomes	958:969	The outcomes of this study	958:983	Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments.
36619394	3	46	theme	viscoelastic	556:567	arg1	properties					569:578	The viscoelastic properties	552:578	The viscoelastic properties	552:578	Methods: The viscoelastic properties, printability, and microstructure of pectin gels crosslinked with CaCO3 were investigated and their composition was optimized (i.e., by including cell culture medium, HEPES buffer, and collagen).
36619394	5	47	dep	Results	934:940	arg1	propose					985:991	propose	985:991	propose	985:991	Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments.
36619394	6	48	theme	further	1177:1183	arg1	parameter					1185:1193	a further parameter	1175:1193	a further parameter to be controlled	1175:1210	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	6	48	theme	further	1177:1183	arg1	pH					1169:1170	pH	1169:1170	pH	1169:1170	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	6	49	theme	pH-dependent	1246:1257	arg1	kinetics					1273:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	multiple (pH-dependent) crosslinking kinetics	1236:1280	By introducing pH as a further parameter to be controlled, it is possible to have multiple (pH-dependent) crosslinking kinetics, without varying hydrogel composition.
36619394	2	50	theme	useful	307:312	arg1	information					314:324	useful information	307:324	useful information	307:324	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	2	51	theme	key	482:484	arg1	pH					474:475	pH	474:475	pH	474:475	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	2	51	theme	key	482:484	arg1	parameter					486:494	a key parameter	480:494	a key parameter to be controlled, together with printing time	480:540	Moving from the concept that rheology provides useful information to predict ink printability, this study improves and expands the potential of the previously published 3D-reactive printing approach by introducing pH as a key parameter to be controlled, together with printing time.
36619394	5	52	theme	controllable	1002:1013	arg1	method					1015:1020	a highly controllable method	993:1020	a highly controllable method to optimize the printability of internally crosslinked polysaccharides	993:1091	Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments.
36619394	1	53	theme	3D-bioprinting	119:132	arg1	view					111:114	the view	107:114	the view of 3D-bioprinting with cell models representative of neural cells	107:180	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
36619394	5	54	theme	post-printing	1128:1140	arg1	treatments					1142:1151	post-printing treatments	1128:1151	post-printing treatments	1128:1151	Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments.
36619394	8	55	theme	neural	1590:1595	arg1	culture					1602:1608	neural cell culture	1590:1608	neural cell culture	1590:1608	Taken together, the results suggest that pectin-based hydrogels could be successfully applied for neural cell culture.
36619394	4	56	dep	neurons	856:862	arg1	i.e.					850:853	i.e.	850:853	i.e.	850:853	Different cell models representative of the major brain cell populations (i.e., neurons, astrocytes, microglial cells, and oligodendrocytes) were considered.
36619394	5	57	theme	polysaccharides	1077:1091	arg1	printability					1038:1049	the printability	1034:1049	the printability of internally crosslinked polysaccharides	1034:1091	Results and Discussion: The outcomes of this study propose a highly controllable method to optimize the printability of internally crosslinked polysaccharides, without the need for additives or post-printing treatments.
36619394	1	58	theme	basic	213:217	arg1	properties					232:241	the basic viscoelastic properties	209:241	the basic viscoelastic properties of brain tissue	209:257	Introduction: In the view of 3D-bioprinting with cell models representative of neural cells, we produced inks to mimic the basic viscoelastic properties of brain tissue.
35956439	11	0	from	applications	1772:1783	arg1	pharmaceuticals					1788:1802	pharmaceuticals	1788:1802	pharmaceuticals	1788:1802	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	11	0	from	applications	1772:1783	arg1	industries					1813:1822	food industries	1808:1822	food industries	1808:1822	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	7	1	theme	highest	873:879	arg1	contents					891:898	the highest flavonoid contents	869:898	the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW)	869:924	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	1	theme	highest	873:879	arg1	DW					922:923	20.11 ± 0.85 mg QE/g DW	901:923	20.11 ± 0.85 mg QE/g DW	901:923	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	4	2	dep	extracts	439:446	arg1	ethanol					384:390	ethanol	384:390	ethanol	384:390	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	2	dep	extracts	439:446	arg1	methanol					418:425	methanol	418:425	methanol	418:425	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	2	dep	extracts	439:446	arg1	acetate					399:405	ethyl acetate	393:405	ethyl acetate	393:405	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	2	dep	extracts	439:446	arg1	water					432:436	water	432:436	water	432:436	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	2	dep	extracts	439:446	arg1	n-hexane					408:415	n-hexane	408:415	n-hexane	408:415	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	2	dep	extracts	439:446	arg1	extracts					439:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts	350:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf	350:473	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	8	3	theme	60.30	1093:1097	arg1	%					1105:1105	60.30 ± 0.52%	1093:1105	60.30 ± 0.52%	1093:1105	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	4	4	theme	ethyl	393:397	arg1	acetate					399:405	ethyl acetate	393:405	ethyl acetate	393:405	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	4	theme	ethyl	393:397	arg1	extracts					439:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts	350:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf	350:473	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	10	5	theme	component	1520:1528	arg1	analysis					1530:1537	The principal component analysis	1506:1537	The principal component analysis	1506:1537	The principal component analysis revealed a positive correlation between the constituents, bioactivities, and extracts.
35956439	2	6	theme	Apocynum	161:168	arg1	plant					209:213	a traditional medicinal plant	185:213	a traditional medicinal plant used primarily as tea	185:235	Apocynum hendersonii is a traditional medicinal plant used primarily as tea.
35956439	2	6	theme	Apocynum	161:168	arg1	hendersonii					170:180	Apocynum hendersonii	161:180	Apocynum hendersonii	161:180	Apocynum hendersonii is a traditional medicinal plant used primarily as tea.
35956439	9	7	theme	Gram-negative	1342:1354	arg1	bacteria					1374:1381	Gram-negative and Gram-positive bacteria	1342:1381	Gram-negative and Gram-positive bacteria	1342:1381	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	3	8	theme	potential	247:255	arg1	benefit					264:270	a potential health benefit	245:270	a potential health benefit from its rich bioactive substances	245:305	It has a potential health benefit from its rich bioactive substances.
35956439	5	9	dep	Fourier	544:550	arg1	Transform					552:560	Transform	552:560	Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC)	552:704	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	7	10	theme	Methanol	841:848	arg1	extracts					850:857	Methanol extracts	841:857	Methanol extracts	841:857	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	9	11	theme	Gram-positive	1360:1372	arg1	bacteria					1374:1381	Gram-negative and Gram-positive bacteria	1342:1381	Gram-negative and Gram-positive bacteria	1342:1381	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	4	12	theme	polarities	372:381	arg1	ethanol					384:390	ethanol	384:390	ethanol	384:390	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	12	theme	polarities	372:381	arg1	methanol					418:425	methanol	418:425	methanol	418:425	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	12	theme	polarities	372:381	arg1	acetate					399:405	ethyl acetate	393:405	ethyl acetate	393:405	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	12	theme	polarities	372:381	arg1	water					432:436	water	432:436	water	432:436	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	12	theme	polarities	372:381	arg1	n-hexane					408:415	n-hexane	408:415	n-hexane	408:415	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	12	theme	polarities	372:381	arg1	extracts					439:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts	350:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf	350:473	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	5	13	theme	Higher	658:663	arg1	HPLC					700:703	HPLC	700:703	HPLC	700:703	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	5	13	theme	Higher	658:663	arg1	Chromatography					684:697	Higher Performance Liquid Chromatography	658:697	Higher Performance Liquid Chromatography (HPLC)	658:704	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	10	14	theme	positive	1550:1557	arg1	correlation					1559:1569	a positive correlation	1548:1569	a positive correlation between the constituents, bioactivities, and extracts	1548:1623	The principal component analysis revealed a positive correlation between the constituents, bioactivities, and extracts.
35956439	7	15	theme	GE/g	1033:1036	arg1	DW					1038:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	15	theme	GE/g	1033:1036	arg1	polysaccharide					1000:1013	phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	961:1040	polysaccharide	1000:1013	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	6	16	theme	bioactive	765:773	arg1	constituents					775:786	medicinally important bioactive constituents	743:786	medicinally important bioactive constituents	743:786	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	6	16	theme	bioactive	765:773	arg1	polysaccharides					824:838	polysaccharides	824:838	polysaccharides	824:838	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	6	16	theme	bioactive	765:773	arg1	flavonoids					808:817	flavonoids	808:817	flavonoids	808:817	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	6	16	theme	bioactive	765:773	arg1	phenols					799:805	phenols	799:805	phenols	799:805	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	8	17	theme	±	1116:1116	arg1	Fe2+					1128:1131	4.60 ± 0.02 µmol Fe2+	1111:1131	4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP)	1111:1258	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	7	18	theme	2.65	1025:1028	arg1	DW					1038:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	18	theme	2.65	1025:1028	arg1	polysaccharide					1000:1013	phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	961:1040	polysaccharide	1000:1013	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	9	19	theme	highest	1391:1397	arg1	activity					1410:1417	the highest inhibition activity	1387:1417	the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase	1387:1473	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	8	20	theme	µmol	1123:1126	arg1	Fe2+					1128:1131	4.60 ± 0.02 µmol Fe2+	1111:1131	4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP)	1111:1258	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	7	21	theme	mg	983:984	arg1	DW					992:993	9.25 ± 0.03 mg GAE/g DW	971:993	9.25 ± 0.03 mg GAE/g DW	971:993	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	21	theme	mg	983:984	arg1	phenolic					961:968	phenolic	961:968	phenolic	961:968	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	4	22	theme	extracts	439:446	arg1	reactivity					336:345	the reactivity	332:345	the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf	332:473	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	8	23	theme	radical	1190:1196	arg1	assay					1209:1213	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay	1151:1213	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay	1151:1213	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	7	24	theme	polysaccharide	1000:1013	arg1	terms					952:956	terms	952:956	terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	952:1040	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	25	theme	±	976:976	arg1	DW					992:993	9.25 ± 0.03 mg GAE/g DW	971:993	9.25 ± 0.03 mg GAE/g DW	971:993	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	25	theme	±	976:976	arg1	phenolic					961:968	phenolic	961:968	phenolic	961:968	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	3	26	theme	bioactive	286:294	arg1	substances					296:305	its rich bioactive substances	277:305	its rich bioactive substances	277:305	It has a potential health benefit from its rich bioactive substances.
35956439	5	27	theme	Liquid	677:682	arg1	HPLC					700:703	HPLC	700:703	HPLC	700:703	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	5	27	theme	Liquid	677:682	arg1	Chromatography					684:697	Higher Performance Liquid Chromatography	658:697	Higher Performance Liquid Chromatography (HPLC)	658:704	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	8	28	theme	ferric	1219:1224	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	8	28	theme	ferric	1219:1224	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP)	1219:1258	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	0	29	theme	Enzyme	59:64	arg1	Activities					77:86	Enzyme Inhibitory Activities	59:86	Enzyme Inhibitory Activities	59:86	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	5	30	theme	extracts	513:520	arg1	composition					494:504	The phytochemical composition	476:504	The phytochemical composition of the extracts	476:520	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	8	31	theme	antioxidant	1235:1245	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	8	31	theme	antioxidant	1235:1245	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP)	1219:1258	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	11	32	theme	overall	1630:1636	arg1	result					1638:1643	The overall result	1626:1643	The overall result	1626:1643	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	9	33	dep	enzymes	1431:1437	arg1	enzymes					1431:1437	the enzymes	1427:1437	the enzymes tyrosinase and acetylcholinesterase	1427:1473	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	9	33	dep	enzymes	1431:1437	arg1	acetylcholinesterase					1454:1473	acetylcholinesterase	1454:1473	acetylcholinesterase	1454:1473	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	9	33	dep	enzymes	1431:1437	arg1	tyrosinase					1439:1448	tyrosinase	1439:1448	tyrosinase	1439:1448	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	11	34	theme	bioactive	1725:1733	arg1	compounds					1735:1743	antioxidant bioactive compounds	1713:1743	antioxidant bioactive compounds	1713:1743	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	0	35	theme	Various	91:97	arg1	Extracts					107:114	Various Organic Extracts	91:114	Various Organic Extracts	91:114	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	9	36	theme	methanol	1488:1495	arg1	extract					1497:1503	methanol extract	1488:1503	methanol extract	1488:1503	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	7	37	from	contents	891:898	arg1	terms					952:956	terms	952:956	terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	952:1040	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	0	38	theme	Extracts	107:114	arg1	Activities					77:86	Enzyme Inhibitory Activities	59:86	Enzyme Inhibitory Activities	59:86	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	0	38	theme	Extracts	107:114	arg1	Antibacterial					40:52	Antibacterial	40:52	Antibacterial	40:52	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	0	38	theme	Extracts	107:114	arg1	Antioxidant					27:37	Antioxidant	27:37	Antioxidant	27:37	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	0	38	theme	Extracts	107:114	arg1	Composition					14:24	Phytochemical Composition	0:24	Phytochemical Composition	0:24	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	0	39	theme	Phytochemical	0:12	arg1	Composition					14:24	Phytochemical Composition	0:24	Phytochemical Composition	0:24	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	7	40	theme	QE/g	917:920	arg1	contents					891:898	the highest flavonoid contents	869:898	the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW)	869:924	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	40	theme	QE/g	917:920	arg1	DW					922:923	20.11 ± 0.85 mg QE/g DW	901:923	20.11 ± 0.85 mg QE/g DW	901:923	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	4	41	dep	A.	455:456	arg1	hendersonii					458:468	hendersonii	458:468	hendersonii	458:468	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	42	theme	solvents	350:357	arg1	ethanol					384:390	ethanol	384:390	ethanol	384:390	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	42	theme	solvents	350:357	arg1	methanol					418:425	methanol	418:425	methanol	418:425	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	42	theme	solvents	350:357	arg1	acetate					399:405	ethyl acetate	393:405	ethyl acetate	393:405	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	42	theme	solvents	350:357	arg1	water					432:436	water	432:436	water	432:436	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	42	theme	solvents	350:357	arg1	n-hexane					408:415	n-hexane	408:415	n-hexane	408:415	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	42	theme	solvents	350:357	arg1	extracts					439:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts	350:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf	350:473	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	43	theme	different	362:370	arg1	polarities					372:381	different polarities	362:381	different polarities	362:381	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	11	44	theme	food	1808:1811	arg1	industries					1813:1822	food industries	1808:1822	food industries	1808:1822	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	7	45	theme	mg	914:915	arg1	contents					891:898	the highest flavonoid contents	869:898	the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW)	869:924	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	45	theme	mg	914:915	arg1	DW					922:923	20.11 ± 0.85 mg QE/g DW	901:923	20.11 ± 0.85 mg QE/g DW	901:923	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	5	46	theme	phytochemical	480:492	arg1	composition					494:504	The phytochemical composition	476:504	The phytochemical composition of the extracts	476:520	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	11	47	theme	rich	1672:1675	arg1	hendersonii					1655:1665	A. hendersonii	1652:1665	A. hendersonii	1652:1665	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	11	47	theme	rich	1672:1675	arg1	source					1685:1690	a rich natural source	1670:1690	a rich natural source of antimicrobial and antioxidant bioactive compounds	1670:1743	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	7	48	theme	±	907:907	arg1	contents					891:898	the highest flavonoid contents	869:898	the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW)	869:924	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	48	theme	±	907:907	arg1	DW					922:923	20.11 ± 0.85 mg QE/g DW	901:923	20.11 ± 0.85 mg QE/g DW	901:923	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	0	49	from	Apocynum	121:128	arg1	Activities					77:86	Enzyme Inhibitory Activities	59:86	Enzyme Inhibitory Activities	59:86	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	0	49	from	Apocynum	121:128	arg1	Antibacterial					40:52	Antibacterial	40:52	Antibacterial	40:52	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	0	49	from	Apocynum	121:128	arg1	Antioxidant					27:37	Antioxidant	27:37	Antioxidant	27:37	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	0	49	from	Apocynum	121:128	arg1	Composition					14:24	Phytochemical Composition	0:24	Phytochemical Composition	0:24	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	9	50	theme	antibacterial	1313:1325	arg1	action					1327:1332	the maximum antibacterial action	1301:1332	the maximum antibacterial action against Gram-negative and Gram-positive bacteria	1301:1381	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	8	51	theme	antioxidant	1067:1077	arg1	capacity					1079:1086	the highest antioxidant capacity	1055:1086	the highest antioxidant capacity	1055:1086	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	7	52	theme	flavonoid	881:889	arg1	contents					891:898	the highest flavonoid contents	869:898	the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW)	869:924	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	52	theme	flavonoid	881:889	arg1	DW					922:923	20.11 ± 0.85 mg QE/g DW	901:923	20.11 ± 0.85 mg QE/g DW	901:923	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	10	53	theme	principal	1510:1518	arg1	analysis					1530:1537	The principal component analysis	1506:1537	The principal component analysis	1506:1537	The principal component analysis revealed a positive correlation between the constituents, bioactivities, and extracts.
35956439	5	54	theme	Chromatography-Mass	602:620	arg1	GC-MS					636:640	GC-MS	636:640	GC-MS	636:640	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	5	54	theme	Chromatography-Mass	602:620	arg1	Spectrometry					622:633	Gas Chromatography-Mass Spectrometry	598:633	Gas Chromatography-Mass Spectrometry (GC-MS)	598:641	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	8	55	theme	±	1099:1099	arg1	%					1105:1105	60.30 ± 0.52%	1093:1105	60.30 ± 0.52%	1093:1105	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	3	56	theme	health	257:262	arg1	benefit					264:270	a potential health benefit	245:270	a potential health benefit from its rich bioactive substances	245:305	It has a potential health benefit from its rich bioactive substances.
35956439	8	57	theme	g	1137:1137	arg1	DW					1139:1140	g DW	1137:1140	g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP)	1137:1258	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	7	58	theme	mg	1030:1031	arg1	DW					1038:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	58	theme	mg	1030:1031	arg1	polysaccharide					1000:1013	phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	961:1040	polysaccharide	1000:1013	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	6	59	theme	constituents	775:786	arg1	presence					731:738	the presence	727:738	the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides	727:838	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	11	60	theme	future	1765:1770	arg1	applications					1772:1783	future applications	1765:1783	future applications in pharmaceuticals and food industries	1765:1822	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	7	61	theme	±	1023:1023	arg1	DW					1038:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	119.66 ± 2.65 mg GE/g DW	1016:1039	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	61	theme	±	1023:1023	arg1	polysaccharide					1000:1013	phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	961:1040	polysaccharide	1000:1013	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	6	62	theme	important	755:763	arg1	constituents					775:786	medicinally important bioactive constituents	743:786	medicinally important bioactive constituents	743:786	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	6	62	theme	important	755:763	arg1	polysaccharides					824:838	polysaccharides	824:838	polysaccharides	824:838	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	6	62	theme	important	755:763	arg1	flavonoids					808:817	flavonoids	808:817	flavonoids	808:817	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	6	62	theme	important	755:763	arg1	phenols					799:805	phenols	799:805	phenols	799:805	The result revealed the presence of medicinally important bioactive constituents, including phenols, flavonoids, and polysaccharides.
35956439	3	63	theme	rich	281:284	arg1	substances					296:305	its rich bioactive substances	277:305	its rich bioactive substances	277:305	It has a potential health benefit from its rich bioactive substances.
35956439	9	64	theme	inhibition	1399:1408	arg1	activity					1410:1417	the highest inhibition activity	1387:1417	the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase	1387:1473	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	7	65	theme	GAE/g	986:990	arg1	DW					992:993	9.25 ± 0.03 mg GAE/g DW	971:993	9.25 ± 0.03 mg GAE/g DW	971:993	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	65	theme	GAE/g	986:990	arg1	phenolic					961:968	phenolic	961:968	phenolic	961:968	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	5	66	theme	Performance	665:675	arg1	HPLC					700:703	HPLC	700:703	HPLC	700:703	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	5	66	theme	Performance	665:675	arg1	Chromatography					684:697	Higher Performance Liquid Chromatography	658:697	Higher Performance Liquid Chromatography (HPLC)	658:704	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	3	67	contain	has	241:243	arg2	benefit					264:270	a potential health benefit	245:270	a potential health benefit from its rich bioactive substances	245:305	It has a potential health benefit from its rich bioactive substances.
35956439	3	67	contain	has	241:243	arg1	It					238:239	It	238:239	It	238:239	It has a potential health benefit from its rich bioactive substances.
35956439	8	68	theme	scavenging	1198:1207	arg1	assay					1209:1213	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay	1151:1213	a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay	1151:1213	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	11	69	theme	antioxidant	1713:1723	arg1	compounds					1735:1743	antioxidant bioactive compounds	1713:1743	antioxidant bioactive compounds	1713:1743	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	0	70	theme	Inhibitory	66:75	arg1	Activities					77:86	Enzyme Inhibitory Activities	59:86	Enzyme Inhibitory Activities	59:86	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	7	71	from	second-highest	934:947	arg1	terms					952:956	terms	952:956	terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	952:1040	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	11	72	theme	antimicrobial	1695:1707	arg1	hendersonii					1655:1665	A. hendersonii	1652:1665	A. hendersonii	1652:1665	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	11	72	theme	antimicrobial	1695:1707	arg1	source					1685:1690	a rich natural source	1670:1690	a rich natural source of antimicrobial and antioxidant bioactive compounds	1670:1743	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	11	73	used	used	1756:1759	arg2	result					1638:1643	The overall result	1626:1643	The overall result	1626:1643	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	7	74	theme	phenolic	961:968	arg1	terms					952:956	terms	952:956	terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW)	952:1040	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	8	75	theme	reducing	1226:1233	arg1	FRAP					1254:1257	FRAP	1254:1257	FRAP	1254:1257	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	8	75	theme	reducing	1226:1233	arg1	power					1247:1251	ferric reducing antioxidant power	1219:1251	ferric reducing antioxidant power (FRAP)	1219:1258	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	0	76	theme	Organic	99:105	arg1	Extracts					107:114	Various Organic Extracts	91:114	Various Organic Extracts	91:114	Phytochemical Composition, Antioxidant, Antibacterial, and Enzyme Inhibitory Activities of Various Organic Extracts from Apocynum hendersonii (Hook.f.)
35956439	4	77	theme	A.	455:456	arg1	leaf					470:473	the A. hendersonii leaf	451:473	the A. hendersonii leaf	451:473	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	11	78	theme	compounds	1735:1743	arg1	hendersonii					1655:1665	A. hendersonii	1652:1665	A. hendersonii	1652:1665	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	11	78	theme	compounds	1735:1743	arg1	source					1685:1690	a rich natural source	1670:1690	a rich natural source of antimicrobial and antioxidant bioactive compounds	1670:1743	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	5	79	theme	Gas	598:600	arg1	GC-MS					636:640	GC-MS	636:640	GC-MS	636:640	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	5	79	theme	Gas	598:600	arg1	Spectrometry					622:633	Gas Chromatography-Mass Spectrometry	598:633	Gas Chromatography-Mass Spectrometry (GC-MS)	598:641	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	4	80	theme	leaf	470:473	arg1	ethanol					384:390	ethanol	384:390	ethanol	384:390	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	80	theme	leaf	470:473	arg1	methanol					418:425	methanol	418:425	methanol	418:425	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	80	theme	leaf	470:473	arg1	acetate					399:405	ethyl acetate	393:405	ethyl acetate	393:405	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	80	theme	leaf	470:473	arg1	water					432:436	water	432:436	water	432:436	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	80	theme	leaf	470:473	arg1	n-hexane					408:415	n-hexane	408:415	n-hexane	408:415	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	4	80	theme	leaf	470:473	arg1	extracts					439:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts	350:446	solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf	350:473	This study investigated the reactivity of solvents of different polarities (ethanol, ethyl acetate, n-hexane, methanol, and water) extracts of the A. hendersonii leaf.
35956439	5	81	theme	Infrared	562:569	arg1	spectrophotometer					571:587	Infrared spectrophotometer	562:587	Infrared spectrophotometer (FT-IR)	562:595	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	5	81	theme	Infrared	562:569	arg1	FT-IR					590:594	FT-IR	590:594	FT-IR	590:594	The phytochemical composition of the extracts was evaluated using a Fourier Transform Infrared spectrophotometer (FT-IR), Gas Chromatography-Mass Spectrometry (GC-MS), UHPLC-MS, and Higher Performance Liquid Chromatography (HPLC).
35956439	2	82	theme	medicinal	199:207	arg1	plant					209:213	a traditional medicinal plant	185:213	a traditional medicinal plant used primarily as tea	185:235	Apocynum hendersonii is a traditional medicinal plant used primarily as tea.
35956439	2	82	theme	medicinal	199:207	arg1	hendersonii					170:180	Apocynum hendersonii	161:180	Apocynum hendersonii	161:180	Apocynum hendersonii is a traditional medicinal plant used primarily as tea.
35956439	2	83	theme	traditional	187:197	arg1	plant					209:213	a traditional medicinal plant	185:213	a traditional medicinal plant used primarily as tea	185:235	Apocynum hendersonii is a traditional medicinal plant used primarily as tea.
35956439	2	83	theme	traditional	187:197	arg1	hendersonii					170:180	Apocynum hendersonii	161:180	Apocynum hendersonii	161:180	Apocynum hendersonii is a traditional medicinal plant used primarily as tea.
35956439	11	84	theme	natural	1677:1683	arg1	hendersonii					1655:1665	A. hendersonii	1652:1665	A. hendersonii	1652:1665	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	11	84	theme	natural	1677:1683	arg1	source					1685:1690	a rich natural source	1670:1690	a rich natural source of antimicrobial and antioxidant bioactive compounds	1670:1743	The overall result showed A. hendersonii as a rich natural source of antimicrobial and antioxidant bioactive compounds and may be used for future applications in pharmaceuticals and food industries.
35956439	8	85	contain	had	1051:1053	arg1	It					1043:1044	It	1043:1044	It	1043:1044	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	8	85	contain	had	1051:1053	arg2	capacity					1079:1086	the highest antioxidant capacity	1055:1086	the highest antioxidant capacity	1055:1086	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	7	86	theme	0.85	909:912	arg1	contents					891:898	the highest flavonoid contents	869:898	the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW)	869:924	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	7	86	theme	0.85	909:912	arg1	DW					922:923	20.11 ± 0.85 mg QE/g DW	901:923	20.11 ± 0.85 mg QE/g DW	901:923	Methanol extracts exhibited the highest flavonoid contents (20.11 ± 0.85 mg QE/g DW) and the second-highest in terms of phenolic (9.25 ± 0.03 mg GAE/g DW) and polysaccharide (119.66 ± 2.65 mg GE/g DW).
35956439	9	87	theme	Ethanol	1275:1281	arg1	extract					1283:1289	Ethanol extract	1275:1289	Ethanol extract	1275:1289	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35956439	3	88	from	substances	296:305	arg1	benefit					264:270	a potential health benefit	245:270	a potential health benefit from its rich bioactive substances	245:305	It has a potential health benefit from its rich bioactive substances.
35956439	8	89	theme	highest	1059:1065	arg1	capacity					1079:1086	the highest antioxidant capacity	1055:1086	the highest antioxidant capacity	1055:1086	It also had the highest antioxidant capacity with 60.30 ± 0.52% and 4.60 ± 0.02 µmol Fe2+ per g DW based on a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and ferric reducing antioxidant power (FRAP), respectively.
35956439	9	90	theme	maximum	1305:1311	arg1	action					1327:1332	the maximum antibacterial action	1301:1332	the maximum antibacterial action against Gram-negative and Gram-positive bacteria	1301:1381	Ethanol extract displayed the maximum antibacterial action against Gram-negative and Gram-positive bacteria and the highest inhibition activity against the enzymes tyrosinase and acetylcholinesterase, followed by methanol extract.
35185978	0	0	theme	membranaceus	102:113	arg1	var					115:117	Astragalus membranaceus var	91:117	Astragalus membranaceus var	91:117	Nutritional Characterization and Untargeted Metabolomics of Oyster Mushroom Produced Using Astragalus membranaceus var.
35185978	8	1	theme	oyster	684:689	arg1	mushroom					691:698	oyster mushroom	684:698	oyster mushroom (Pleurotus ostreatus var	684:723	Here, oyster mushroom (Pleurotus ostreatus var.
35185978	9	2	theme	basal	755:759	arg1	substrate					761:769	a basal substrate	753:769	a basal substrate supplemented with AMM stems and leaves (AMM group)	753:820	florida) was cultivated on a basal substrate supplemented with AMM stems and leaves (AMM group).
35185978	6	3	from	compounds	479:487	arg1	rich					464:467	rich	464:467	rich	464:467	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	0	4	theme	Astragalus	91:100	arg1	var					115:117	Astragalus membranaceus var	91:117	Astragalus membranaceus var	91:117	Nutritional Characterization and Untargeted Metabolomics of Oyster Mushroom Produced Using Astragalus membranaceus var.
35185978	13	5	from	metabolomics	1361:1372	arg1	group					1402:1406	AMM group	1398:1406	AMM group	1398:1406	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	8	6	dep	mushroom	691:698	arg1	var					721:723	Pleurotus ostreatus var	701:723	oyster mushroom (Pleurotus ostreatus var	684:723	Here, oyster mushroom (Pleurotus ostreatus var.
35185978	14	7	theme	care	1637:1640	arg1	value					1642:1646	very high nutritional therapy health care value	1600:1646	very high nutritional therapy health care value	1600:1646	In summary, our results imply that oyster mushroom cultured with AMM stems and leaves might have very high nutritional therapy health care value.
35185978	7	8	theme	AMM	604:606	arg1	stems					608:612	AMM stems	604:612	AMM stems	604:612	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	11	9	theme	oyster	1096:1101	arg1	mushroom					1103:1110	oyster mushroom	1096:1110	oyster mushroom	1096:1110	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	12	10	dep	annotated	1159:1167	arg1	based					1169:1173	based	1169:1173	annotated based on the metabolomics data, of which 680 were identified as differentially expressed metabolites	1159:1268	Moreover, 2,156 metabolites were detected and annotated based on the metabolomics data, of which 680 were identified as differentially expressed metabolites.
35185978	14	11	theme	health	1630:1635	arg1	value					1642:1646	very high nutritional therapy health care value	1600:1646	very high nutritional therapy health care value	1600:1646	In summary, our results imply that oyster mushroom cultured with AMM stems and leaves might have very high nutritional therapy health care value.
35185978	7	12	theme	waste	584:588	arg1	products					590:597	waste products	584:597	waste products from AMM stems and leaves	584:623	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	7	12	theme	waste	584:588	arg1	substrates					636:645	useful substrates	629:645	useful substrates for edible fungus cultivation	629:675	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	3	13	theme	medicinal	226:234	arg1	material					236:243	an edible and medicinal material	212:243	an edible and medicinal material	212:243	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	3	13	theme	medicinal	226:234	arg1	mongolicus					192:201	mongolicus	192:201	mongolicus (AMM)	192:207	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	11	14	theme	mushroom	1103:1110	arg1	fat					1024:1026	fat	1024:1026	fat	1024:1026	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	11	14	theme	mushroom	1103:1110	arg1	protein					1029:1035	protein	1029:1035	protein	1029:1035	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	11	14	theme	mushroom	1103:1110	arg1	weight					1006:1011	the fresh weight	996:1011	the fresh weight	996:1011	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	11	14	theme	mushroom	1103:1110	arg1	moisture					1014:1021	moisture	1014:1021	moisture	1014:1021	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	11	14	theme	mushroom	1103:1110	arg1	composition					1081:1091	amino acid composition	1070:1091	amino acid composition	1070:1091	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	11	14	theme	mushroom	1103:1110	arg1	concentrations					1050:1063	element concentrations	1042:1063	element concentrations	1042:1063	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	13	15	theme	AMM	1398:1400	arg1	group					1402:1406	AMM group	1398:1406	AMM group	1398:1406	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	9	16	theme	AMM	789:791	arg1	stems					793:797	AMM stems	789:797	AMM stems	789:797	florida) was cultivated on a basal substrate supplemented with AMM stems and leaves (AMM group).
35185978	13	17	theme	Many	1271:1274	arg1	phytometabolites					1283:1298	Many active phytometabolites	1271:1298	Many active phytometabolites previously identified in AMM herbs	1271:1333	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	3	18	used	used	261:264	arg2	AMM					204:206	AMM	204:206	AMM	204:206	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	3	18	used	used	261:264	arg2	mongolicus					192:201	mongolicus	192:201	mongolicus (AMM)	192:207	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	3	18	used	used	261:264	arg2	material					236:243	an edible and medicinal material	212:243	an edible and medicinal material	212:243	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	2	19	theme	Astragalus	163:172	arg1	membranaceus					174:185	Astragalus membranaceus	163:185	Astragalus membranaceus	163:185	Astragalus membranaceus var.
35185978	10	20	theme	chemical	843:850	arg1	composition					852:862	The nutritional and chemical composition	823:862	The nutritional and chemical composition of the fruiting body	823:883	The nutritional and chemical composition of the fruiting body were analyzed by metabolomics and chemometrics.
35185978	3	21	theme	edible	215:220	arg1	material					236:243	an edible and medicinal material	212:243	an edible and medicinal material	212:243	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	3	21	theme	edible	215:220	arg1	mongolicus					192:201	mongolicus	192:201	mongolicus (AMM)	192:207	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	11	22	theme	fresh	1000:1004	arg1	weight					1006:1011	the fresh weight	996:1011	the fresh weight	996:1011	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	0	23	theme	Nutritional	0:10	arg1	Characterization					12:27	Nutritional Characterization	0:27	Nutritional Characterization	0:27	Nutritional Characterization and Untargeted Metabolomics of Oyster Mushroom Produced Using Astragalus membranaceus var.
35185978	13	24	theme	fatty	1490:1494	arg1	acids					1496:1500	3 fatty acids	1488:1500	3 fatty acids	1488:1500	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	14	25	theme	high	1605:1608	arg1	value					1642:1646	very high nutritional therapy health care value	1600:1646	very high nutritional therapy health care value	1600:1646	In summary, our results imply that oyster mushroom cultured with AMM stems and leaves might have very high nutritional therapy health care value.
35185978	0	26	theme	Untargeted	33:42	arg1	Metabolomics					44:55	Untargeted Metabolomics	33:55	Untargeted Metabolomics	33:55	Nutritional Characterization and Untargeted Metabolomics of Oyster Mushroom Produced Using Astragalus membranaceus var.
35185978	10	27	theme	body	880:883	arg1	composition					852:862	The nutritional and chemical composition	823:862	The nutritional and chemical composition of the fruiting body	823:883	The nutritional and chemical composition of the fruiting body were analyzed by metabolomics and chemometrics.
35185978	14	28	theme	oyster	1538:1543	arg1	mushroom					1545:1552	oyster mushroom	1538:1552	oyster mushroom	1538:1552	In summary, our results imply that oyster mushroom cultured with AMM stems and leaves might have very high nutritional therapy health care value.
35185978	2	29	dep	var	187:189	arg1	membranaceus					174:185	Astragalus membranaceus	163:185	Astragalus membranaceus	163:185	Astragalus membranaceus var.
35185978	13	30	from	group	1402:1406	arg1	acids					1496:1500	3 fatty acids	1488:1500	3 fatty acids	1488:1500	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	30	from	group	1402:1406	arg1	flavonoids					1437:1446	21 flavonoids	1434:1446	21 flavonoids	1434:1446	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	30	from	group	1402:1406	arg1	metabolomics					1361:1372	the metabolomics	1357:1372	the metabolomics	1357:1372	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	30	from	group	1402:1406	arg1	phenylpropanoids					1466:1481	14 phenylpropanoids	1463:1481	14 phenylpropanoids	1463:1481	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	30	from	group	1402:1406	arg1	alkaloids					1452:1460	17 alkaloids	1449:1460	17 alkaloids	1449:1460	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	30	from	group	1402:1406	arg1	mushroom					1384:1391	oyster mushroom	1377:1391	oyster mushroom from AMM group	1377:1406	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	30	from	group	1402:1406	arg1	terpenoids					1422:1431	46 terpenoids	1419:1431	46 terpenoids	1419:1431	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	7	31	theme	fungus	658:663	arg1	cultivation					665:675	edible fungus cultivation	651:675	edible fungus cultivation	651:675	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	10	32	theme	fruiting	871:878	arg1	body					880:883	the fruiting body	867:883	the fruiting body	867:883	The nutritional and chemical composition of the fruiting body were analyzed by metabolomics and chemometrics.
35185978	12	33	theme	metabolomics	1182:1193	arg1	data					1195:1198	the metabolomics data	1178:1198	the metabolomics data	1178:1198	Moreover, 2,156 metabolites were detected and annotated based on the metabolomics data, of which 680 were identified as differentially expressed metabolites.
35185978	6	34	from	rich	464:467	arg1	terpenoids					522:531	terpenoids	522:531	terpenoids	522:531	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	34	from	rich	464:467	arg1	polysaccharides					538:552	polysaccharides	538:552	polysaccharides	538:552	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	34	from	rich	464:467	arg1	flavonoids					510:519	flavonoids	510:519	flavonoids	510:519	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	34	from	rich	464:467	arg1	compounds					479:487	active compounds	472:487	active compounds	472:487	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	34	from	rich	464:467	arg1	saponins					500:507	saponins	500:507	saponins	500:507	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	3	35	theme	East	269:272	arg1	Asia					274:277	East Asia	269:277	East Asia	269:277	mongolicus (AMM) is an edible and medicinal material and is commonly used in East Asia.
35185978	8	36	dep	Pleurotus	701:709	arg1	ostreatus					711:719	ostreatus	711:719	ostreatus	711:719	Here, oyster mushroom (Pleurotus ostreatus var.
35185978	11	37	theme	element	1042:1048	arg1	concentrations					1050:1063	element concentrations	1042:1063	element concentrations	1042:1063	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	12	38	theme	2,156	1123:1127	arg1	metabolites					1129:1139	2,156 metabolites	1123:1139	2,156 metabolites	1123:1139	Moreover, 2,156 metabolites were detected and annotated based on the metabolomics data, of which 680 were identified as differentially expressed metabolites.
35185978	14	39	theme	therapy	1622:1628	arg1	value					1642:1646	very high nutritional therapy health care value	1600:1646	very high nutritional therapy health care value	1600:1646	In summary, our results imply that oyster mushroom cultured with AMM stems and leaves might have very high nutritional therapy health care value.
35185978	6	40	theme	active	472:477	arg1	terpenoids					522:531	terpenoids	522:531	terpenoids	522:531	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	40	theme	active	472:477	arg1	polysaccharides					538:552	polysaccharides	538:552	polysaccharides	538:552	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	40	theme	active	472:477	arg1	flavonoids					510:519	flavonoids	510:519	flavonoids	510:519	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	40	theme	active	472:477	arg1	compounds					479:487	active compounds	472:487	active compounds	472:487	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	6	40	theme	active	472:477	arg1	saponins					500:507	saponins	500:507	saponins	500:507	The stems and leaves are also rich in active compounds, including saponins, flavonoids, terpenoids, and polysaccharides.
35185978	0	41	theme	Mushroom	67:74	arg1	Characterization					12:27	Nutritional Characterization	0:27	Nutritional Characterization	0:27	Nutritional Characterization and Untargeted Metabolomics of Oyster Mushroom Produced Using Astragalus membranaceus var.
35185978	0	41	theme	Mushroom	67:74	arg1	Metabolomics					44:55	Untargeted Metabolomics	33:55	Untargeted Metabolomics	33:55	Nutritional Characterization and Untargeted Metabolomics of Oyster Mushroom Produced Using Astragalus membranaceus var.
35185978	7	42	theme	edible	651:656	arg1	cultivation					665:675	edible fungus cultivation	651:675	edible fungus cultivation	651:675	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	13	43	theme	oyster	1377:1382	arg1	mushroom					1384:1391	oyster mushroom	1377:1391	oyster mushroom from AMM group	1377:1406	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	0	44	theme	Oyster	60:65	arg1	Mushroom					67:74	Oyster Mushroom	60:74	Oyster Mushroom	60:74	Nutritional Characterization and Untargeted Metabolomics of Oyster Mushroom Produced Using Astragalus membranaceus var.
35185978	7	45	theme	useful	629:634	arg1	substrates					636:645	useful substrates	629:645	useful substrates for edible fungus cultivation	629:675	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	7	45	theme	useful	629:634	arg1	products					590:597	waste products	584:597	waste products from AMM stems and leaves	584:623	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	13	46	located	detected	1345:1352	arg1	acids					1496:1500	3 fatty acids	1488:1500	3 fatty acids	1488:1500	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	46	located	detected	1345:1352	arg1	flavonoids					1437:1446	21 flavonoids	1434:1446	21 flavonoids	1434:1446	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	46	located	detected	1345:1352	arg1	metabolomics					1361:1372	the metabolomics	1357:1372	the metabolomics	1357:1372	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	46	located	detected	1345:1352	arg1	phenylpropanoids					1466:1481	14 phenylpropanoids	1463:1481	14 phenylpropanoids	1463:1481	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	46	located	detected	1345:1352	arg1	alkaloids					1452:1460	17 alkaloids	1449:1460	17 alkaloids	1449:1460	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	46	located	detected	1345:1352	arg2	phytometabolites					1283:1298	Many active phytometabolites	1271:1298	Many active phytometabolites previously identified in AMM herbs	1271:1333	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	46	located	detected	1345:1352	arg1	terpenoids					1422:1431	46 terpenoids	1419:1431	46 terpenoids	1419:1431	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	47	theme	mushroom	1384:1391	arg1	acids					1496:1500	3 fatty acids	1488:1500	3 fatty acids	1488:1500	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	47	theme	mushroom	1384:1391	arg1	flavonoids					1437:1446	21 flavonoids	1434:1446	21 flavonoids	1434:1446	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	47	theme	mushroom	1384:1391	arg1	metabolomics					1361:1372	the metabolomics	1357:1372	the metabolomics	1357:1372	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	47	theme	mushroom	1384:1391	arg1	phenylpropanoids					1466:1481	14 phenylpropanoids	1463:1481	14 phenylpropanoids	1463:1481	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	47	theme	mushroom	1384:1391	arg1	alkaloids					1452:1460	17 alkaloids	1449:1460	17 alkaloids	1449:1460	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	13	47	theme	mushroom	1384:1391	arg1	terpenoids					1422:1431	46 terpenoids	1419:1431	46 terpenoids	1419:1431	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	8	48	theme	Pleurotus	701:709	arg1	var					721:723	Pleurotus ostreatus var	701:723	oyster mushroom (Pleurotus ostreatus var	684:723	Here, oyster mushroom (Pleurotus ostreatus var.
35185978	11	49	theme	amino	1070:1074	arg1	composition					1081:1091	amino acid composition	1070:1091	amino acid composition	1070:1091	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	4	50	theme	AMM	338:340	arg1	medicinal					345:353	medicinal	345:353	medicinal	345:353	According to the pharmacopeia of China, the dried root of AMM is medicinal.
35185978	4	50	theme	AMM	338:340	arg1	root					330:333	the dried root	320:333	the dried root of AMM	320:340	According to the pharmacopeia of China, the dried root of AMM is medicinal.
35185978	13	51	theme	AMM	1325:1327	arg1	herbs					1329:1333	AMM herbs	1325:1333	AMM herbs	1325:1333	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	11	52	theme	acid	1076:1079	arg1	composition					1081:1091	amino acid composition	1070:1091	amino acid composition	1070:1091	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	11	53	theme	AMM	957:959	arg1	addition					961:968	AMM addition	957:968	AMM addition to the substrate	957:985	Our results showed that AMM addition to the substrate affected the fresh weight, moisture, fat, protein, and element concentrations, and amino acid composition of oyster mushroom.
35185978	14	54	theme	nutritional	1610:1620	arg1	value					1642:1646	very high nutritional therapy health care value	1600:1646	very high nutritional therapy health care value	1600:1646	In summary, our results imply that oyster mushroom cultured with AMM stems and leaves might have very high nutritional therapy health care value.
35185978	5	55	theme	aerial	369:374	arg1	parts					376:380	the aerial parts	365:380	the aerial parts of AMM	365:387	However, the aerial parts of AMM are always directly discarded after harvest.
35185978	7	56	from	leaves	618:623	arg1	products					590:597	waste products	584:597	waste products from AMM stems and leaves	584:623	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	7	56	from	leaves	618:623	arg1	substrates					636:645	useful substrates	629:645	useful substrates for edible fungus cultivation	629:675	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	4	57	theme	China	313:317	arg1	pharmacopeia					297:308	the pharmacopeia	293:308	the pharmacopeia of China	293:317	According to the pharmacopeia of China, the dried root of AMM is medicinal.
35185978	13	58	theme	active	1276:1281	arg1	phytometabolites					1283:1298	Many active phytometabolites	1271:1298	Many active phytometabolites previously identified in AMM herbs	1271:1333	Many active phytometabolites previously identified in AMM herbs were also detected in the metabolomics of oyster mushroom from AMM group, including 46 terpenoids, 21 flavonoids, 17 alkaloids, 14 phenylpropanoids, and 3 fatty acids.
35185978	7	59	from	stems	608:612	arg1	products					590:597	waste products	584:597	waste products from AMM stems and leaves	584:623	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	7	59	from	stems	608:612	arg1	substrates					636:645	useful substrates	629:645	useful substrates for edible fungus cultivation	629:675	To rationally use resources, waste products from AMM stems and leaves are useful substrates for edible fungus cultivation.
35185978	14	60	theme	AMM	1568:1570	arg1	stems					1572:1576	AMM stems	1568:1576	AMM stems	1568:1576	In summary, our results imply that oyster mushroom cultured with AMM stems and leaves might have very high nutritional therapy health care value.
35185978	12	61	theme	expressed	1248:1256	arg1	680					1210:1212	680	1210:1212	680	1210:1212	Moreover, 2,156 metabolites were detected and annotated based on the metabolomics data, of which 680 were identified as differentially expressed metabolites.
35185978	12	61	theme	expressed	1248:1256	arg1	metabolites					1258:1268	differentially expressed metabolites	1233:1268	differentially expressed metabolites	1233:1268	Moreover, 2,156 metabolites were detected and annotated based on the metabolomics data, of which 680 were identified as differentially expressed metabolites.
35185978	4	62	theme	dried	324:328	arg1	medicinal					345:353	medicinal	345:353	medicinal	345:353	According to the pharmacopeia of China, the dried root of AMM is medicinal.
35185978	4	62	theme	dried	324:328	arg1	root					330:333	the dried root	320:333	the dried root of AMM	320:340	According to the pharmacopeia of China, the dried root of AMM is medicinal.
35185978	10	63	theme	nutritional	827:837	arg1	composition					852:862	The nutritional and chemical composition	823:862	The nutritional and chemical composition of the fruiting body	823:883	The nutritional and chemical composition of the fruiting body were analyzed by metabolomics and chemometrics.
35185978	5	64	theme	AMM	385:387	arg1	parts					376:380	the aerial parts	365:380	the aerial parts of AMM	365:387	However, the aerial parts of AMM are always directly discarded after harvest.